LOCUS       DATCRO010000001         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_843876, whole genome shotgun sequence.
ACCESSION   DATCRO010000001 DATCRO010000000
VERSION     DATCRO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843876"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VIN75_00005"
     CDS             complement(<1..1237)
                     /locus_tag="VIN75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY8972566.1"
                     /translation="MFAGLWLAVGQVFAQASAPPRPEPALVQVAPGQQAIRLAHAGVD
                     VFVEGSLARTVIDLTLSNPNARVLEGELQFPLHAGQTVTGFALDMADGSMRAAVPVPK
                     ARGREVFEAVVRRGVDPALLEQTAGENFRLRVYPLRPGGTRHVRITLTEWLTPDANQR
                     LRLGLPLDFAGSAADQLDLHVRLHATASAQVTLGAALDGASITTQGPDTDIGLHRAAF
                     RPAAGDRHRLDLSWLAAHGDSVLRERAGDEAYFHAELKVPDQPAARARPDELTLIWDA
                     SGSGAQRDHEAELRLLAALFAWQADVKVRLRIVRDAAEPDRMFDVRRGDWQALREVLR
                     HMPYDGASNASLWTAPAGRSPSTSLALLFSDGLGNWGDPTPQAAGAVPAYAVQAGAGG
                     SGVFLRQWAEARGGRLLDLA"
     gene            complement(1337..2083)
                     /gene="rlmB"
                     /locus_tag="VIN75_00010"
     CDS             complement(1337..2083)
                     /gene="rlmB"
                     /locus_tag="VIN75_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY8972567.1"
                     /translation="MSRPHVLFGFHAITVRMKTAPQSIVELHVETQRRDARMRAFAER
                     ARDAGIKIVDSDGERLDKMAGSPRHQGVVARVEAVKMPHSLDEVVENIEGPPLLLVLD
                     GVTDPHNLGACLRVADGSGTHAVVAPKDHAVGINATVAKVASGAAETVPYIMVTNLAR
                     TLTELKEHDIRIIGTSDDAEHTLYDLDLTGPVAFVLGAEGDGMRQLTRKTCDQLVRIP
                     MAGAVESLNVSVASAVCLYEALRQRAKAAV"
     gene            complement(2080..>2367)
                     /locus_tag="VIN75_00015"
     CDS             complement(2080..>2367)
                     /locus_tag="VIN75_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY8972568.1"
                     /translation="VHSPLLDGRLGNWTGLVSRLPVTEDYVPVFPWLGMMLAGYLAAS
                     LLLVRARAAFAGGVPFLVRPLAVLGRWSLSFYMIHQLVLIGALELLRKILP"
BASE COUNT          332 a          831 c          888 g          316 t
ORIGIN      
        1 ctgccagatc gagcagccgc ccgccgcgag cctcggccca ttggcgcagg aagacgcccg
       61 aaccgccggc gcctgcctgg acggcgtagg ccggcaccgc gccggccgct tgcggcgtcg
      121 gatcgcccca gttgcccagg ccgtcggaga acagcagcgc caggctcgtg gatggcgagc
      181 ggccggccgg cgcggtccaa agcgacgcgt tgctcgcgcc gtcgtagggc atgtggcgca
      241 gcacctcgcg caaggcctgc cagtcgccgc ggcgcacgtc gaacatccgg tcgggctcgg
      301 cggcgtcgcg cacgatgcgc agccgcacct tgacgtcggc ctgccacgcg aacagcgcgg
      361 cgagcagccg cagctcggcc tcgtggtcgc gctgcgcgcc ggagcccgac gcgtcccaga
      421 tcagcgtcag ttcgtcgggc cgggcgcgcg ccgccggctg atccggcacc ttcagctcgg
      481 catggaagta cgcctcgtcg ccggcgcgtt cgcggagcac ggagtcgcca tgcgccgcga
      541 gccaggagag gtcgagccga tggcggtcgc cggcggctgg acggaacgcg gcgcggtgca
      601 ggccgatgtc ggtgtccggg ccctgcgtcg tgatgctcgc gccgtccagg gccgctccca
      661 aggtcacctg ggcggacgcc gtggcgtgca ggcgaacgtg caggtccagc tggtcggcgg
      721 cgctgccggc gaagtccagc ggcaggccca ggcgcaggcg ctggttcgcg tcgggcgtca
      781 gccattcggt gagcgtgatg cggacgtggc gcgtgccacc cgggcgcagc ggatagacac
      841 gcagccggaa gttctcgccg gccgtctgtt cgagcagcgc cggatcgacg ccgcggcgca
      901 cgacggcctc gaacacctcg cggccgcgcg ccttgggcac cggcaccgcg gcgcgcatgc
      961 tgccgtcggc catgtccagc gcgaacccgg tgacggtctg gccggcgtgc agcgggaact
     1021 gcaactcgcc ttccaggacg cgggcgttcg ggttggacag cgtcaggtcg atgaccgtgc
     1081 gggccaggct gccctcgacg aacacgtcga cgccggcgtg cgccaggcgg atggcctgct
     1141 ggccgggcgc gacctgcacc agcgcgggct cgggccgcgg cggcgccgag gcctgcgcga
     1201 acacctggcc caccgcgagc cacaggcccg cgaacacgat tgctcttgcg cgttgcatct
     1261 cgttccccgt cgtccaaagg cgatgggggt tgaacggacg ccccgcgcga acggggtcaa
     1321 gcggcgtcga atatcgtcag accgcggcct tggcgcgctg gcgcagcgcc tcgtacaggc
     1381 agacggccga ggccaccgac acgttcaggc tctcgacagc gccggccatc gggatgcgca
     1441 ccagctggtc gcaggtcttg cgcgtcagct ggcgcatgcc gtcgccctcg gcgcccagca
     1501 cgaaggcgac cgggcccgtc aggtccaggt cgtacagcgt atgctcggcg tcgtcgctgg
     1561 tgccgatgat gcggatgtca tgttccttga gctcggtcag cgtgcgcgcg aggttggtga
     1621 ccatgatgta gggcaccgtc tcggccgcgc cgctggccac cttggcgacc gtggcgttga
     1681 tgcccaccgc gtggtccttg ggcgcgacga ccgcgtgcgt gcccgagccg tcggccacgc
     1741 gcaggcaggc gccgaggttg tgcgggtcgg tcacgccgtc cagcaccagc agcagcggcg
     1801 ggccctcgat gttctcgacc acctcgtcca gcgagtgcgg catcttgacg gcctccacgc
     1861 gcgcgaccac gccctggtgg cgcgggctgc cggccatctt gtcgaggcgc tcgccgtcgg
     1921 agtcgacgat cttgatgccc gcgtcacgcg cgcgctccgc gaacgcgcgc atgcgcgcgt
     1981 cgcgccgctg ggtctcgacg tgcagttcga cgatggattg cggggcggtc ttcatgcgca
     2041 cggtgatcgc atggaagccg aagaggacgt gggggcggct catgggagga tcttcctgag
     2101 aagttccagc gcgccgatca ggacgagctg gtggatcatg tagaagctga gcgaccagcg
     2161 gccgaggacg gccagcggtc gaacgaggaa gggaacgccg cccgcgaagg cggcgcgcgc
     2221 gcgcaccagc agcaggctgg ccgcgaggta gccggcgagc atcatgccca gccacgggaa
     2281 gaccggcacg tagtcctccg tgaccggcag gcgcgacacg aggcccgtcc agttgcccag
     2341 gcggccgtcg agcaacgggc tgtgcac
//
LOCUS       DATCRO010000002         7582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_348846, whole genome shotgun sequence.
ACCESSION   DATCRO010000002 DATCRO010000000
VERSION     DATCRO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348846"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VIN75_00020"
     CDS             complement(<1..315)
                     /locus_tag="VIN75_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8972569.1"
                     /translation="MYTAKGQLDANAMLKQYSPLVRRLAHQMIAKLPANVEIDDLIQV
                     GMIGLNDAMSRFDAEQGVQFETFATQRIRGAMLDELRGTDWMSRGGRRQQREIETAVH
                     KLE"
     gene            complement(336..1151)
                     /locus_tag="VIN75_00025"
     CDS             complement(336..1151)
                     /locus_tag="VIN75_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HEY8972570.1"
                     /translation="MTSRRRNPPPTDQADGLRRMFAASQGVLVPVVANPHVEHHGAVL
                     ARLASVFAEQGLNTLVVDAADTAPEPGELAFIDLGTCVEPLAPQLAYLAARGLPMQFV
                     DTRGSCASFVPRMMSVVQDVDVVVMHAEARVLARMLGEQPLRPVLLASGVGESITHAY
                     AALKLLVQRLGLMSFDLVMPSNKGRGPTPAQVSRRVSDCADRFLGAALHDSATIDPDI
                     AFGAASGTDLMRLAAAQLRVPAAASAVAPVASAADPVWTTEQTARALNSFLSA"
     gene            complement(1217..2917)
                     /gene="flhF"
                     /locus_tag="VIN75_00030"
     CDS             complement(1217..2917)
                     /gene="flhF"
                     /locus_tag="VIN75_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEY8972571.1"
                     /translation="MNVKRFAARTSREALALVRQAFGVDAVVLSTKPCAEGIEVLAMA
                     PDSVAGLERLTAEPEAPAAAPRRGPISQRAAAAAAPAPVQNAGLAGAQRELATRMGVP
                     QNEVEADADQLAMSTLSFQDYVRDRMLKRRHAELNNQPVEAAPTRAPETTRSALAQTS
                     AARTAMSRAAERQQQMQAIDANDDHFDAPAPAPQQRRAAPQRVEPTLPPQRAERASPM
                     SFANAPTLHDDYMEEAPAPQAASVTHLADVVSRNNHMDMVSELRSMKGLIEERFGALA
                     FMEKLQRQPRQAMLAQKLLDVGFSPALIRKMVDALPANVADENAWAAGVLERNLLTSE
                     GELAMEDQGGVYALIGSTGVGKTTTTAKIAAGFAARHGAANLGLITLDAYRVGAHEQL
                     RAYGRILGVPVHTAHDRASLEDLLELLAGKKMVLIDTAGMAQRDGRTRELLEMLAHRS
                     IKKVLVVDASSQGETIDDVIVNWKANACAGVILSKIDEAVKLAPALDALIRHKLKNIG
                     VANGQRVPEDWHHLSANALVHRALKGGGSAAYRMDPQDVHLVFAHAGVSQHSAAQLRV
                     "
     gene            complement(2914..5010)
                     /gene="flhA"
                     /locus_tag="VIN75_00035"
     CDS             complement(2914..5010)
                     /gene="flhA"
                     /locus_tag="VIN75_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY8972572.1"
                     /translation="MDQIKNLLASVTGGNAALGKALMAPIFIVMVLSMMVLPLPSFVL
                     DLFFTFNIAMALMVMLVSANLVRPLDFAAFPTVLLITTLLRLSLNVASTRVVLMEGHT
                     GPGAAGHVIESFGHFLIGGNFAVGLIVFAILVVINFIVVTKGAERIAEVGARFTLDAM
                     PGKQMAIDADLNAGLIDEAMAKQRRREVGDEAEFFGSMDGASKFVRGDAMAGILILFI
                     NIVGGFIIGVVQHGLSAGEAADSYILLAVGDALVAQIPGLLISVAAAMVVSRVGKEED
                     SGSQIRAQLFGSPKAMGITAAIVGALALIPGMPHLVFMIIAGAAGWFAHFQTRKAARD
                     AAAAKARADVPPPPKPADAEAGWDDVTPVDVLGLEVGYRLIALVDKSRSGDLLARIKG
                     VRKKFAQDVGFLPPSVHIRDNLELKPSMYRVTLRGAVIGEGEAFPGMFLAINPGGTTL
                     ALPGTKCVDPAFGLPAVWIEERQRESAQMSGYTVVDCPTVVATHLSHLMQVNAARLLG
                     RNEVQMLVEHVTKLAPKLIEDVVPKMIAIAPLQKVLQLLLEEGVHIRDFRSIVESLAE
                     HASTVTDPAELARRIRTHIAPAIVQQIYGPVRELDVIALEPDLERMVTQALTSPHGAV
                     LDPGVADTLTRQASDMARRQEDKGTPACLLVPDALRAPLARLLKRAAPRLKVLAHSEI
                     PETHSIRIGSIIGGTA"
     gene            complement(5032..6204)
                     /gene="flhB"
                     /locus_tag="VIN75_00040"
     CDS             complement(5032..6204)
                     /gene="flhB"
                     /locus_tag="VIN75_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY8972573.1"
                     /translation="MAGQDSADRNLPASAKKIRKARQEGNVPRSRDLGHFVAMGLAAG
                     LLFTVMPSLTGYAQRLLSDGLTFDHRVIADPRLAGEFALGLFTRGLMVVLMVGGLFMA
                     AAVGSNVAFGGWNLTMKAVSPNFGKLNPISGFGRIFNFQQLLNVLKASALAIAVGFAG
                     TTYLRSHFADLGGVLTAELPDGLATVGRTLGGGFLAMLGVLAVWAMVDVPWQKKQFMD
                     KLKMSREEVKQEHKESEGNVEVKAKIKQKMREMAKRRMMKAVPVADIVVMNPTHYAVA
                     LKYDQEAMGAPRVVAKGTDRLAMRIRDLARDAGVPVLEAPPLARALYTHVEIDGEVPA
                     MLFAAVAQVLAWVYQLRAAPNLNLRAPDVSVPPELDPLTGGPKPKRRRPGPRAAAA"
     gene            6431..6844
                     /gene="panD"
                     /locus_tag="VIN75_00045"
     CDS             6431..6844
                     /gene="panD"
                     /locus_tag="VIN75_00045"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY8972574.1"
                     /translation="MFRTLLKSKIHRARVTHCELHYEGSCAIDEDLLDASNIRENEQV
                     HIWDVDNGERLVTYALRAPRGSGIVSLNGSAARRAAVGDLVIIAAFAQVHEDKLDDAE
                     PRLVFVDADNGNRIKPRPPFVGHTEETVAAAKSAK"
     gene            6906..>7582
                     /locus_tag="VIN75_00050"
     CDS             6906..>7582
                     /locus_tag="VIN75_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8972575.1"
                     /translation="MTSTLTGSNDAWRARSLRAVWHPCTQMKAHGAPDGPAADSTPLV
                     AIASAEGPWLVDVEGKRYLDGVSSWWTNLFGHGHPAIRAALVDQLSRLDHTMLAGLTH
                     APVVELSERLAALTGLGHAFYASDGASATEIALKMSAHHWRNAGKPAKNRFVALQGGY
                     HGETAGALGVTDIALFREAYAPLVRLAATVASPDARGAQGGESAADVAHRAARDLAQW
                     LARHHQET"
BASE COUNT         1112 a         2573 c         2744 g         1153 t
ORIGIN      
        1 ctcgagcttg tgcaccgccg tctcgatctc gcgctgctgg cggcggccgc cgcggctcat
       61 ccagtcggtg ccgcgcagct cgtcgagcat cgcgccacgg atgcgctgcg tcgcgaaggt
      121 ctcgaactgc acgccctgct cggcgtcgaa gcgcgacatc gcgtcgttca ggccgatcat
      181 gcccacctgg atcaggtcgt cgatctcgac gttggccggc agcttggcaa tcatctggtg
      241 cgcaagtcgg cgcacgagcg ggctgtattg cttgagcatc gcgttggcgt cgagctggcc
      301 tttggcggtg tacatggcga tctccggatt cttgttcaag cggacaaaaa ggaattcagg
      361 gcgcgggccg tctgctcggt cgtccagaca ggatcggcgg ccgaggcgac gggcgcgacc
      421 gccgaggcgg cggccgggac gcgcagctgc gcggcggcca ggcgcatcag gtcggtaccc
      481 gaggccgcgc cgaaggcgat gtcggggtcg atcgtggccg agtcgtgcaa tgcggcgccc
      541 aggaagcggt cggcgcagtc ggacacgcgg cgcgaaacct gcgccggcgt cgggccgcgg
      601 cccttgttcg aaggcatcac gaggtcgaag ctcatgaggc cgaggcgctg caccagcagc
      661 ttgagcgcgg cgtacgcgtg cgtgatgctc tcgcccacgc ccgaggccag cagcaccgga
      721 cgcagcggct gctcgcccag catgcgggcc agcacgcgcg cctcggcgtg catgacgacg
      781 acgtcgacgt cctgcacgac gctcatcatg cgcggcacga agctcgcgca cgaaccgcgc
      841 gtgtcgacga actgcatcgg caggccgcgc gcggcgaggt aggccagttg cggggccagc
      901 ggctcgacgc aggtgcccag gtcgatgaag gcgagctcgc cgggttcggg cgcggtgtct
      961 gccgcatcca cgaccagcgt gttcagcccc tgctcggcga acacgctcgc gagccgtgcc
     1021 agcacggcgc cgtggtgctc gacgtgcggg ttcgcgacga ccggcaccag cacgccctgg
     1081 ctcgcggcga acatgcggcg caggccgtcg gcctggtcgg tcggaggagg gttgcgacga
     1141 cgggatgtca tgtaagcaac tatgtccgtg cgggcgttga tgcgatcggg cgaaaagcgg
     1201 tgtttcgtcg cggtgttcag acgcgcagct gcgcggccga atgctggctc acgccggcgt
     1261 gcgcgaacac gaggtgcacg tcctgcggat ccatgcggta ggccgcgctg ccgccgccct
     1321 tgagcgcgcg atgcacgagc gcgttggccg acaggtggtg ccagtcttcc ggcacgcgct
     1381 ggccgttggc cacgccgatg ttcttgagct tgtggcggat cagcgcgtcg agcgccggag
     1441 ccagcttcac ggcctcgtcg atcttggaca ggatcacgcc ggcgcaggcg ttcgccttcc
     1501 agttgacgat cacgtcgtcg atggtctcgc cctgcgacga ggcgtcgacc accagcacct
     1561 tcttgatcga gcggtgcgcc agcatctcca gcagctcgcg ggtgcggccg tcgcgctggg
     1621 ccatgccggc cgtgtcgatc agcaccatct tcttgccggc cagcagctcg agcaggtctt
     1681 ccagcgacgc gcggtcgtgc gcggtgtgca cgggcacgcc caggatgcgg ccataggcgc
     1741 gcagctgttc gtgggcgccg acgcggtagg cgtccagcgt gatcaggccg aggttggcgg
     1801 cgccgtggcg cgcggcgaag ccggcggcga tcttggcggt ggtcgtggtc ttgccgacgc
     1861 cggtggagcc gatcagcgcg tacacgccgc cctggtcctc catcgcgagc tcgccttcgc
     1921 tggtgagcag gttgcgttcc agcacgccgg cggcccaggc gttctcgtcg gccacgttgg
     1981 cgggcagcgc gtcgaccatc ttgcggatca gcgccggcga gaagccgacg tccagcagct
     2041 tctgcgcgag catcgcctgg cgcggctggc gctgcagctt ttccatgaag gccagggcgc
     2101 cgaagcgttc ctcgatcagg cccttcatcg agcgcagctc gctcaccatg tccatgtggt
     2161 tgttgcggct gacgacgtcg gccaggtgcg tcaccgacgc ggcctgcggc gcgggcgcct
     2221 cctccatgta gtcgtcgtgc agcgtcggcg cgttggcgaa cgacatcggc gacgcacgct
     2281 cggcgcgctg cggcggcagg gtcggctcga ctcgctgcgg cgcggcgcgg cgttgctgcg
     2341 gggctggcgc gggagcgtcg aagtggtcgt cgttggcgtc gatggcctgc atctgctgct
     2401 ggcgctcggc ggcgcgcgac atcgccgtgc gggcggcgct ggtctgcgcg agcgcgctgc
     2461 gcgtggtctc gggcgcgcgg gtcggggcgg cctcgaccgg ctggttgttg agttcggcgt
     2521 ggcggcgctt gagcatgcgg tcgcgcacgt agtcctggaa cgacagcgtg ctcatcgcga
     2581 gctggtcggc gtcggcctcc acctcgttct gcggcacgcc catgcgggtg gcgagttcgc
     2641 gctgcgcacc ggccaggccg gcgttctgca cgggcgccgg cgcggccgcg gcggcggcgc
     2701 gctgcgagat cgggccacgg cgcggtgcgg cggccggtgc ttccggttcg gcggtgaggc
     2761 gctcgaggcc ggcgacgctg tcgggcgcca tcgcgagcac ttcgatccct tcggcgcacg
     2821 gcttcgtcga gagcaccacg gcatcgacgc cgaaggcctg gcgcaccagc gcgagggcct
     2881 cgcgcgaagt gcgggcagca aagcgtttga cgttcatgcg gtacctccga tgatcgagcc
     2941 gatccggatc gagtgggttt cgggaatttc actgtgagcc agcaccttca ggcgcggggc
     3001 ggctcgcttg agcagtcggg ccagcggggc gcgcagcgcg tccggcacca gcaggcacgc
     3061 cggcgtgccc ttgtcttcct ggcggcgggc catgtcggag gcctggcgcg tcagggtgtc
     3121 ggccacgccc gggtccagca ccgcgccgtg gggcgacgtg agggcctggg tgaccatgcg
     3181 ttcgaggtcg ggttccagcg cgatgacgtc cagctcgcgc accgggccgt agatctgctg
     3241 gacgatcgcc ggggcgatgt gcgtacggat gcggcgggcc agttcggccg gatcggtgac
     3301 ggtggaggcg tgctccgcca gcgattcgac gatcgagcgg aaatcgcgga tgtgcacgcc
     3361 ctcttccagc agcagctgga ggactttttg cagcggtgcg atggcgatca tcttcgggac
     3421 gacgtcttcg atcagtttgg gggccagctt ggtcacgtgc tcgaccagca tctggacttc
     3481 gttgcggccc agcaggcgag ccgcgttcac ttgcatcaag tgtgagagat gggtggccac
     3541 gacggtcggg caatcaacga ccgtataccc actcatttgg gcactttcgc gctggcgctc
     3601 ctcgatccag acggccggca gcccgaaggc gggatccacg cacttggtgc ccggcagcgc
     3661 gagcgtcgtg ccgcccgggt tgatcgccag gaacatgccc gggaaggcct cgccctcgcc
     3721 gatgacggcg ccgcgcagcg tgacgcggta catgctgggc ttgagctcca ggttgtcgcg
     3781 gatgtgcacc gaaggcggca ggaagccgac gtcttgcgcg aacttcttgc gcacgccctt
     3841 gatgcgggcg agcaggtcgc ccgagcggct cttgtcgacc agcgcgatca ggcggtagcc
     3901 cacttccagg ccgaggacgt cgaccggggt gacgtcgtcc cagcccgctt ccgcgtcggc
     3961 cggcttgggc ggcggcggca cgtcggccct ggccttggcg gcggcggcgt cgcgcgcggc
     4021 cttcctggtc tggaagtgcg cgaaccagcc ggcggcgccc gcgatgatca tgaagaccag
     4081 gtgcggcatg cccgggatca gcgccagcgc gccgacgatc gccgcggtga tgcccatcgc
     4141 cttgggcgag ccgaacagct gggcgcggat ctgcgagccc gaatcctctt ccttgccgac
     4201 gcgcgacacg accatggccg cggccaccga gatcagcagg ccggggatct gcgcgaccag
     4261 cgcgtcgcca accgccagca ggatgtacga atcggccgcc tcgccggccg acaggccatg
     4321 ctggacgacg ccgatgatga agccgccgac gatgttgatg aacaggatca ggatgcccgc
     4381 catcgcgtcg ccgcgcacga acttcgaggc accgtccatg gagccgaaga actcggcctc
     4441 gtcgccgact tcgcggcggc gctgcttggc catcgcctcg tcgatcaggc cggcgttcag
     4501 gtcggcgtcg atggccatct gcttgccggg catcgcgtcc agggtgaagc gcgcgccgac
     4561 ttcggcgatg cgctcggcgc ccttggtgac cacgatgaag ttgatcacga ccaggatcgc
     4621 gaacacgatc aggccgacgg cgaagttgcc gccgatcagg aagtggccga acgactcgat
     4681 gacgtggccg gcggcgcctg ggccggtgtg gccttccatc agcaccacgc gggtggaggc
     4741 gacgttgagc gacaggcgca gcagcgtcgt gatcagcagc acggtgggaa acgcggcgaa
     4801 gtccagcggg cgcaccaggt tggccgacac cagcatcacc atcagcgcca tcgcgatgtt
     4861 gaacgtgaag aacaggtcca gcacgaacga cggcagcggc agcaccatca tggacagcac
     4921 catgacgatg aagatcggcg ccatcagggc cttgccgagc gcggcgttgc cgccggtgac
     4981 ggatgcgagg aggttcttga tctggtccat cgaagggctc tggtgcttcg cttacgcggc
     5041 cgcggcgcgc gggccggggc gacgacgctt gggcttgggg ccgccggtga gcggatcgag
     5101 ttcgggcggc acgctgacgt ccggcgcgcg caggttcagg ttcggggcgg cgcgcagctg
     5161 gtacacccag gccagcacct gcgccacggc ggcgaacagc atggccggca cctcgccgtc
     5221 gatctcgacg tgggtgtaga gcgcgcgcgc gagcggcggg gcctcgagca ccggcacgcc
     5281 ggcgtcgcgg gcgaggtcgc ggatgcgcat cgcgagtcgg tcggtgccct tggcgacgac
     5341 gcgcggggcg cccatcgcct cctggtcgta cttgagcgcg accgcgtagt gggtcgggtt
     5401 catcacgacg atgtcggcca cgggaaccgc cttcatcatg cggcgcttgg ccatctcgcg
     5461 catcttctgc ttgatcttgg ccttgacctc gacgttgccc tcggactcct tgtgctcctg
     5521 cttgacttcc tcgcgggaca tcttgagctt gtccatgaac tgcttcttct gccagggcac
     5581 gtcgaccatc gcccacaccg cgagcacgcc cagcatcgcc aggaagccgc cgcccagcgt
     5641 gcggccgacc gtggccaggc catcgggcag ctcggcggtg agcacgccgc ccaggtcggc
     5701 gaagtggctg cgcaggtagg tggtccccgc gaagccgacc gcgatcgcca gcgccgacgc
     5761 cttcaggacg ttgagcagct gctggaagtt gaagatgcgg ccgaagcccg agatcgggtt
     5821 cagcttgccg aagttggggc tgacggcctt catcgtcagg ttccagccgc cgaaggcgac
     5881 gttggagccg acggcggccg ccatgaacag gccgccgacc atcagcacga ccatcaggcc
     5941 gcgcgtgaac aggcccagcg cgaactcgcc ggccaggcga gggtcggcga tcacgcggtg
     6001 gtcgaaggtc agcccgtcgc tcagcaggcg ctgcgcgtag cccgtcagcg acggcatcac
     6061 ggtgaacagc aggcccgcgg ccaggcccat ggcgacgaaa tgccccaggt cccgcgagcg
     6121 cgggacattg ccttcctggc gcgccttgcg aattttcttc gccgaggcgg gcaggttgcg
     6181 gtctgcggag tcttggccgg ccatggaggg ggtggaaacg gagttcgggc agcgttttgg
     6241 tgtgacgaca tgttgccggg ccgccccgga aacttgagcg ggaaaagagc ccgaaatcca
     6301 gcctcatccc ggccgacttc gccgcagcca aagcccttgc cgcacccgga cgaaagcgtc
     6361 cagatggaga agccgggcaa aatcccgtct tttccccgat tgcgccgacg ttccaggcgc
     6421 cccagaaccg atgttccgca ccctgctcaa atccaagatc caccgtgccc gcgtcacgca
     6481 ctgcgagctg cactacgagg gctcgtgcgc catcgacgag gacctgctgg acgcctcgaa
     6541 catccgcgag aacgagcagg tgcacatctg ggacgtggac aacggcgagc gcctggtcac
     6601 ctacgccctg cgcgccccgc gcggcagcgg catcgtctcg ctcaacggct cggccgcccg
     6661 ccgcgcggcc gtcggcgatc tcgtgatcat tgccgcgttc gcccaggtgc acgaggacaa
     6721 gctcgacgac gccgagccca ggctcgtctt cgtcgacgcc gacaacggca accgcatcaa
     6781 gccgcgcccg ccgttcgtcg gccacaccga agagaccgtc gcggccgcca agtccgcgaa
     6841 atgagcgcaa gcacggccgc tccgatgggc acccgcgccg cagcccgcac gggcggaggc
     6901 tcgcgatgac ctcgacgctc acgggctcca acgacgcctg gcgggcgcgc agcctgcgcg
     6961 ccgtctggca tccgtgcacg cagatgaagg cgcacggcgc gccggacggc ccggcggccg
     7021 attcgacgcc gctggtggcc atcgcgtcgg ccgaggggcc ctggctggtc gacgtcgaag
     7081 gaaagcgcta tctcgacggc gtcagctcgt ggtggaccaa cctgttcggc cacggccacc
     7141 cggcgatccg ggcggcgctg gtcgaccagt tgtcgcgcct ggaccacacg atgctggcgg
     7201 gcttgacgca cgcccccgtg gtcgagttgt cggaacgcct ggccgccctc accggcctgg
     7261 gccacgcgtt ctatgccagc gacggcgcct cggccaccga gatcgccctg aagatgagcg
     7321 cgcatcactg gcgcaacgcc ggcaagcccg ccaagaaccg cttcgtcgcg ctgcagggtg
     7381 gctaccacgg cgagaccgcg ggcgcgctcg gcgtgaccga catcgcgctg ttccgcgagg
     7441 cctatgcgcc gctggtgcgg ctcgcggcca cggtcgcctc gcccgacgcg cgcggcgcgc
     7501 agggcggcga gagcgccgcc gacgtcgcgc accgcgccgc ccgcgatctc gcgcagtggc
     7561 tcgcccggca ccaccaggag ac
//
LOCUS       DATCRO010000003         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_191337, whole genome shotgun sequence.
ACCESSION   DATCRO010000003 DATCRO010000000
VERSION     DATCRO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191337"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VIN75_00055"
     CDS             complement(<1..337)
                     /locus_tag="VIN75_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8972576.1"
                     /translation="MSARSTRGAPPPSELALARVVGVHGRHSVIETPDGRRLLAHGRG
                     KKSETVVGDHVRWQVSGDEGVIEAIEPRTSLLKRQDEWRSKNFAANIDLLLVMVATDP
                     PYSESQLARA"
     gene            complement(334..1626)
                     /locus_tag="VIN75_00060"
     CDS             complement(334..1626)
                     /locus_tag="VIN75_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8972577.1"
                     /translation="MNPVVLSLAFSAAILASLLIKFWLATRQMRHVAAHRGAVPVAFA
                     ATVPLAAHQKAADYTLAKLRLGIVGEAFGAAVLIAWTLLGGLAWLNGLVQDLVLARWG
                     GMAYQLLLIGAYVLIGGLIDLPFDLYRTFRLEQRFGFNKTTPALYVTDLVKSTLLGIA
                     IGGPLLAAVLWIMQATGAHWWVWAWVALSAFQLLAMVIYPTVIAPMFNKFEPLADEAL
                     VGRVQALMQRCGFRAKGFFVMDGSRRSAHANAYFTGFGAAKRVVFFDTLLKRLNGDEV
                     EAVLAHELGHFAHRHIAKRVVLMLAFSLAGFALLGWLATQWGFYTGLGVTPHLMASND
                     ALALLLFMMGLPPFMFFVSPVFASLSRRDEYQADAYARSNANAADLVRALLKLYEDNA
                     STLTPDPLYVRFYYSHPPASSRIAALGLADVPLAGVPA"
     gene            1860..>2240
                     /locus_tag="VIN75_00065"
     CDS             1860..>2240
                     /locus_tag="VIN75_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972578.1"
                     /translation="MSNHDIEGRVPPEATTDAANPQSGAPDSLERQLAREREARQLIR
                     DRLRHTEALAAESHALRSKMAEELERVTADRDRLRAQAQIAAAVAVPDPAPTAPTPPG
                     REPVAAASARPPAAPPAADLRERPA"
BASE COUNT          371 a          775 c          818 g          276 t
ORIGIN      
        1 gggcacgcgc cagctgcgat tcgctgtagg gcgggtcggt ggccaccatc accaggagca
       61 ggtcgatgtt ggcggcgaag ttcttgctgc gccattcgtc ctggcgcttc aggaggctgg
      121 tgcgcggttc gatcgcctcg atcacgccct cgtcgccgga cacctgccag cgcacatggt
      181 cgcccaccac cgtctcgctc ttcttgccgc ggccgtgcgc gagcagccgc cggccgtcgg
      241 gcgtctcgat gacggaatgg cgcccatgca cgccgacgac ccgtgccagc gccagctcgg
      301 aaggcggcgg cgcgccgcgc gtcgagcgtg cgctcaagcc ggcactccgg cgagcggcac
      361 gtcggccagg cccagcgccg cgatgcgcga cgaggccggc gggtgcgagt agtagaagcg
      421 cacgtacagc gggtcgggcg tcagcgtcga cgcgttgtcc tcgtacagct tgagcagcgc
      481 gcgcaccagg tcggcggcgt tggcgttgct gcgcgcgtag gcgtcggcct ggtactcgtc
      541 gcggcgcgac aggctggcga acaccggcga cacgaagaac atgaacggcg gcaggcccat
      601 catgaacagc agcagcgcca gcgcgtcgtt gctggccatc aggtggggcg tcacgcccag
      661 ccccgtgtag aagccccatt gcgtggcgag ccagccgagc agcgcgaagc ccgcgaggct
      721 gaacgcgagc atcaacacca cgcgcttggc gatgtggcga tgcgcgaagt ggcccagctc
      781 gtgcgcgagc accgcctcga cctcgtcgcc gttcaggcgc ttgagcagcg tgtcgaagaa
      841 gacgacgcgc ttggccgcgc cgaagcccgt gaagtacgcg ttggcgtggg ccgagcggcg
      901 gctgccgtcc atcacgaaga agcccttggc gcgaaagccg cagcgctgca tcagcgcctg
      961 cacgcggccg accagcgcct cgtcggcgag cggctcgaac ttgttgaaca tcggcgcgat
     1021 gaccgtcggg tagatgacca tcgccagcag ctggaacgcc gacaacgcca cccaggccca
     1081 gacccaccag tgcgcgccgg tggcctgcat gatccacagc acggcggcca gcagcgggcc
     1141 gccgatggcg atgcccagca gcgtcgactt gacgagatcg gtgacgtaca gcgccggcgt
     1201 ggtcttgttg aagccgaagc gctgctcgag gcggaacgtg cggtaaaggt cgaacggcag
     1261 gtcgatcagt ccgccgatga gcacgtaggc tccgatgagc agcaactggt aggccatgcc
     1321 gccccagcgc gccaggacca gatcctggac gaggccgttg agccacgcga ggccgcccag
     1381 cagcgtccat gcgatgagca ccgcggcgcc gaaggcctcg ccgacgatgc ccagacgcag
     1441 cttggcgagc gtgtagtccg ccgccttctg gtgcgccgcg agcggcacgg tggcggcgaa
     1501 cgcgacgggg accgcgccgc gatgggcggc cacgtggcgc atctggcggg tggccagcca
     1561 gaacttgatg agcagcgagg ccaggatcgc ggccgagaac gccagggaca ggacgacggg
     1621 attcatggtt cggaagtgta ggcgcttggc cgccgggcgc ccggcgccgc aagcggggga
     1681 aagaccctgt tcggcgacgg gcaagccgat tgcagatggg gcccgaaccg tcgagcgcaa
     1741 gggtcgcacg gttgacagct tgttacggct gcgcccgcgc tcggcgccgc cgcaatgccg
     1801 tgcgcggtct accatttccg gcaaggagga tgggagccga cacgcgatgg agaagcgctg
     1861 tgagcaacca cgatatcgag ggccgagtcc cgcccgaggc gacgacggac gccgccaacc
     1921 cgcaatccgg cgcaccggat tccctggaac gccagctcgc cagggagcgt gaggcgcgcc
     1981 agctcatccg cgaccggctg cgccataccg aggcgctggc ggccgagtcg cacgcgctgc
     2041 gctcgaagat ggccgaggag ctggaacgcg tgacggccga ccgcgaccgc ctgcgtgcgc
     2101 aagcgcagat cgcggcggcg gtcgccgtgc ccgatccggc tccgaccgcg ccgacgccgc
     2161 ccgggcgcga acccgtggcc gccgcgagcg cccggccgcc ggccgcgcca cccgcggcgg
     2221 acctgcgtga acgtcccgcc
//
LOCUS       DATCRO010000004         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_776412, whole genome shotgun sequence.
ACCESSION   DATCRO010000004 DATCRO010000000
VERSION     DATCRO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776412"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..2306
                     /locus_tag="VIN75_00070"
     CDS             444..2306
                     /locus_tag="VIN75_00070"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY8972579.1"
                     /translation="MKDSPWSPASASEPRLLADIGATYARFCVEDSPGHFNHVVVYPC
                     ADFAGFADVVQAYLRSLEGTPPRHAAIAIANPINGDAVQMTNRDWAFSIERVRQELGL
                     ATLLVVNNFTALAMALPGLQHGQRVQVGGGEAQPDGAIALLGPGTGLGVSGLVPAEDR
                     WSTLATEGGHVSFAPNDERELKVLQHTWKHLSHVSAERLISGPGIELIHEALTSGTAQ
                     GGQRLSTAEIVRRAVARSDAASVEAIDCFCGMLGSFASNLALTLGATGGVYIGGGIVP
                     RLGELFERSQFRARFEAKGRFTSWLAKIPTYVLTADNPAFRGLSLLLAEHVPDTSSHS
                     PLLDRVRSARDTLTPAERRVADVVLGNPRSLLNDAIAVIAAQAEVSQPTVIRFCRSLG
                     FEGLADFKLKLASGLTGTIPVLRSQVRRGEPTADLSAKVLDNTISAIVHFRESLNSAA
                     IDRAINLLRSARRVEIFACGNSAVVALDAQHKLLRFRIPAISQTDTSLQGLAAEMLAP
                     GDVAVFISSSGQTAELVRAAGIAIERGAGVIAITASQSRLARLATVCIGLDHAEDSST
                     FLSMVARILQLLVVDIMSVGLAVQRDPNPGRPDAATEGGNRNAPAPGVRISHLS"
     gene            complement(2415..>3314)
                     /locus_tag="VIN75_00075"
     CDS             complement(2415..>3314)
                     /locus_tag="VIN75_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HEY8972580.1"
                     /translation="ALVEESLKEALDFRRAMRKIDAELEGGWWFKVWGPEKLAEDGVG
                     EAKDWVLEANESWHGFGNLAPGFNMVDPIKSTIITPGLGVNGDFDKRGIPASIVTKYL
                     AEHGIVVEKTGLYSFFIMFTIGITKGRWNTLLATLQQFKDDYDKNAQLWRILPEFSAA
                     HPRYERMGLRDLCQAIHEVYTQGDIARLTTDMYLSDLQPAMKPSEAYARIAHRDTERV
                     AIDALEGRITTSLLTPYPPGIPLLIPGERFNRKILAYLQFVREFNERFPGFDTDVHGL
                     VIEEDNFGRKHYFVDCVRDTPSH"
BASE COUNT          495 a         1198 c         1084 g          537 t
ORIGIN      
        1 cttacccgtt gagtttttca ccagcatgtc tcctaagttc tgcggatgac gcggggtgcg
       61 ccaccgtcga tacgcagtgt ttgtagtcaa acgaccccca cttgcgaaga gccgaaaatc
      121 ggatgtatta aaactagatc accagattct cgactgaagt ttactgatct tttacgtgct
      181 tgcaaggggg ccacctgggt aaaacgccgc gtttttgcat cgatgacacg ggtagaaaca
      241 cgggggtccc ggtccacgaa cctgtccgac tgctggttca ccccaggacg cagatgtagc
      301 ccaattactt atcgttgggg agttttgtag ctgccgtagg gctacgcggg cgccgtgccc
      361 accgtggaac cgtttccgcc cactgcgaaa acttggtccg caggcacgac aatcagtgca
      421 tgaacgcgga aagcacccaa gacgtgaaag acagcccctg gtccccggcc tccgcctcgg
      481 agccgcgact gctcgccgac atcggcgcca cctacgcgcg cttctgcgtc gaggattcgc
      541 ccggccactt caatcacgtg gtggtctacc cgtgcgccga cttcgccggc ttcgccgacg
      601 tggtgcaggc ctacctgcgc agcctcgagg gcacgccgcc gcgccacgcc gccatcgcca
      661 tcgccaaccc gatcaacggc gacgcggtgc agatgaccaa ccgcgactgg gcgttctcga
      721 tcgagcgcgt gcgccaggag ctggggctgg ccaccctgct ggtcgtcaac aacttcaccg
      781 cgctggcgat ggccctgccc ggcctgcagc acggccagcg cgtgcaggtg ggcggcggcg
      841 aggcgcagcc cgacggcgcg atcgcgctgc tgggccccgg cacgggcctg ggcgtctcgg
      901 gcctggtgcc ggcggaggac cgctggtcga cgctggccac cgagggcggc cacgtcagct
      961 tcgcgccgaa cgacgagcgc gagctcaagg tgctgcagca cacctggaag cacctgtcgc
     1021 acgtctcggc cgagcgcctg atctccggcc ccggcatcga gctcatccac gaggcgctca
     1081 cctccggcac cgcccagggc ggccagcgac tgagcaccgc cgagatcgtg cgccgcgcgg
     1141 tcgcccgctc cgacgccgcc agcgtcgagg cgatcgactg cttctgcggc atgctgggct
     1201 cgttcgcgtc caacctcgcg ctgacgctcg gcgccaccgg cggcgtctac atcggcggcg
     1261 gcatcgtgcc gcgcctgggc gagctgttcg agcgctcgca gttccgcgcc cgcttcgagg
     1321 ccaagggccg attcacgtcc tggctggcca agattcccac ctatgtcctg acggccgaca
     1381 acccggcctt ccgcggcctg tcgctgctgc tggccgagca cgtgcccgac accagctccc
     1441 acagcccgct gctcgatcgc gtgcgcagcg cgcgcgacac gctcacgccg gccgagcgcc
     1501 gcgtcgccga cgtcgtgctg ggcaacccgc gctcgctgct gaacgacgcc atcgccgtga
     1561 tcgcggcgca ggccgaggtc agccaaccca ccgtcatccg cttctgccgc tccctgggct
     1621 tcgagggcct ggccgacttc aagctgaagc tcgcctccgg cctgacgggc acgatccccg
     1681 tgctgcgcag ccaggtgcgc cgcggcgagc cgacggccga cctcagcgcc aaggtgctgg
     1741 acaacacgat ctcggcgatc gtgcacttcc gcgagtcgct gaactcggcg gcgatcgacc
     1801 gcgccatcaa cctgctgcgc tcggcgcgcc gggtggagat cttcgcctgc ggcaactccg
     1861 ccgtcgtggc gctcgacgcc cagcacaagc tgctgcgctt ccgcatcccg gccatctcgc
     1921 agaccgacac ctcgctgcag ggcctggccg ccgagatgct ggcgccgggc gacgtggccg
     1981 tcttcatctc gagctcgggc cagacggccg agctcgtgcg cgccgcgggc atcgccatcg
     2041 agcgcggcgc gggcgtcatc gccatcaccg cgagccagtc gcgcctcgcg cgcctggcca
     2101 ccgtgtgcat cggcctggac cacgccgagg acagctccac gttcctgtcg atggtggcgc
     2161 gcatcctgca attgctggtg gtcgacatca tgtcggtggg cctggccgtc cagcgcgacc
     2221 cgaacccggg ccgcccggac gcggccaccg aaggcggcaa ccgcaacgcc ccggcgccgg
     2281 gcgtgcggat ctcgcacctg agctgaggcg gcgggcgccc gcctgcgccc gccaagccgt
     2341 catcctgcgc gaagtcgcag gatccacccc ccgccaaccc ggcggtcgtt cacggccgcc
     2401 cgcccgcgtg cgggtcagtg cgacggcgtg tcccgcacgc aatccacgaa gtagtgcttg
     2461 cgcccgaagt tgtcttcctc gatcaccagc ccatgcacgt cggtgtcgaa gcccgggaag
     2521 cgctcgttga actcgcgcac gaactgcagg taggcgagga tcttgcggtt gaagcgctcg
     2581 cccgggatca gcagcgggat gcccggcggg tacggcgtca gcagcgaggt ggtgatgcgg
     2641 ccttccagcg cgtcgatggc cacgcgctcg gtgtcgcggt gcgcgatgcg ggcgtaggct
     2701 tccgacggct tcatggccgg ctgcaggtcc gacaggtaca tgtcggtcgt caggcgcgcg
     2761 atgtcgccct gcgtgtagac ctcgtggatg gcctggcaca ggtcgcgcag gcccatgcgc
     2821 tcgtatcgcg ggtgcgcggc gctgaactcc ggcaggatgc gccacagctg ggcgttcttg
     2881 tcgtagtcgt ccttgaactg ctgcagcgtg gccagcagcg tgttccagcg gcccttggtg
     2941 atgccgatcg tgaacatgat gaagaacgag tacaggcccg tcttctccac cacgatgccg
     3001 tgctcggcca ggtacttggt gacgatggac gcggggatgc cgcgcttgtc gaagtcgccg
     3061 ttgacgccca ggcccggggt gatgatcgtg ctcttgatcg ggtcgaccat gttgaagccc
     3121 ggcgccaggt tgccgaagcc gtgccacgac tcgttggcct cgagcaccca gtccttggcc
     3181 tcgcccacgc cgtcctcggc cagcttctcg ggcccccaga ccttgaacca ccagccgccc
     3241 tcgagctcgg cgtcgatctt gcgcatggcg cggcggaagt cgagcgcctc cttcaggctc
     3301 tcctcgacca gcgc
//
LOCUS       DATCRO010000005         7053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_461388, whole genome shotgun sequence.
ACCESSION   DATCRO010000005 DATCRO010000000
VERSION     DATCRO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461388"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2875)
                     /gene="acnA"
                     /locus_tag="VIN75_00080"
     CDS             complement(116..2875)
                     /gene="acnA"
                     /locus_tag="VIN75_00080"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8972581.1"
                     /translation="MPLAATAPFAHTLQTFKLPSGTSFRFHSLPALAAELPQVNRLPV
                     SIRIVLEAVLRHCDGRRITPEHVRQLASWAPTGERSEEIPFIVARVVLQDFTGVPLLC
                     DLAAMRTEAANQKKNPKTIEPLVPVDLVVDHSVMIDDYAHKNSLDINMKIEFQRNNER
                     YKFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKHETGDGKPPVYYPDTLVGTD
                     SHTTMINGLGVVGWGVGGIEAEAAMLGQPVYFLTPDVVGFEFTGQLREGVTATDLVLT
                     VTEQLRKAKVVGKFVEFHGAGTASLSVPDRATLANMAPEYGATMGFFAVDDKTVEYLR
                     GTGRTNEEIEAFEAYFRAQGLFGTPASGSIDYSEVLTLDLGTVAPSLAGPKRPQDRIE
                     IGNLSRKFDELLTTPAASNGYNLPPEAVTRRAVTPSGVEIGHGDVLIAAITSCTNTSN
                     PSVMLAAGLLAKKAVAAGLRVKPHVKTSLAPGSRIVTEYLQKAGLLPALEQLGFNVAA
                     YGCTTCIGNAGDLTPEINQAIVENDLVAAAVLSGNRNFEARIHPNLKANFLASPPLVL
                     AYAIAGNVKVDLMTEPVGKGTGGKDVWLGDIWPTSQEVHDLMKFAMDADSFRANYGQV
                     KSNPGALWNDITGVTGQVYDWPRSTYIARPPFFDGFTQVPQAAPTSVKGARIMALFGD
                     SITTDHISPAGSIKSDSPAGRYLQANGVLPADFNSYGSRRGNHEVMMRGTFANVRIKN
                     LMLPARADGGRVEGGYTIYQGPATADLPADSSKPVPIYDAAMAYQAHGIPTVVFAGEE
                     YGTGSSRDWAAKGTQLLGIKAVVARSFERIHRNNLVGMGVLPLQFKAGVTWSSLGITG
                     NETIDIEWPAPITPQSEATLTIHRADGKSQKVPLVLRIDTPIEVDYYAHGGILPYVLR
                     QLLAA"
     gene            complement(3034..3432)
                     /gene="arfB"
                     /locus_tag="VIN75_00085"
     CDS             complement(3034..3432)
                     /gene="arfB"
                     /locus_tag="VIN75_00085"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY8972582.1"
                     /translation="MPIAIATDEVEITAIRAQGAGGQNVNKVSSAVHLRFDVKASSLP
                     EGVKARLLALRDHRITEDGVVVIKAQTHRSQDMNRAEALQRLAELVASVEKPPVKRRP
                     TRPTLGSQRRRLEAKSVRSAVKAGRGKVDG"
     gene            complement(3490..4419)
                     /locus_tag="VIN75_00090"
     CDS             complement(3490..4419)
                     /locus_tag="VIN75_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972583.1"
                     /translation="MNVIKRAALAAALSAVASASFAQTLNPAQVALLRWYPSTGSLAA
                     VPMTNALGIAFDGAHVWVTSFSGNTVSKIRAADGAVLGTYATGGFPTDVAFDGANIWV
                     TNQSASSVSKIRASDGALQGTYATGSAPTGIAYDGNYIWTANYYGASVTKIRVSNGAV
                     VGTYPVGANPLSVAFDGTNVWVVNSASGTVSKLSQATGATIGTYSVGTARGVAYDGTN
                     IWVVGGNVATRLRASDGAVLGTTPIGNGAYTAVFDGTYIWTANGTDKTVTKILASTGV
                     AVGSFPTPGTGPNYLAFDGANVWATVGSGAAKL"
     gene            complement(4539..5603)
                     /locus_tag="VIN75_00095"
     CDS             complement(4539..5603)
                     /locus_tag="VIN75_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8972584.1"
                     /translation="MRILDLRLRLRALGALPEHEARVLRQWVNAQPQDAGKRRLADFM
                     PLAVRDALPALATELAGLATLRSEHPGEDGSARLLVGLCDGQTVESVLLPRDGLCVST
                     QVGCAVGCVFCMTGRDGLLRQVGSAEIVAQVALARSRRLVRKVVFMGMGEPAHNLDNV
                     MEAIDLLGTLGGIGHKNLVFSTVGDPRVFERLPQGPVKPALALSLHSTRDALRRELLP
                     RAPRMSPEELVDAGERYARATGYPIQYQWTLLEGVNDGDDEIEGIVRLLKGKYALMNM
                     IPYNTNEGLGFARPAPEKAAAIARTLHSRGVLTKLRQSAGQDVEGGCGQLRARALDGG
                     ALVRMPVSRGRREAPSARPD"
     gene            complement(5751..6716)
                     /locus_tag="VIN75_00100"
     CDS             complement(5751..6716)
                     /locus_tag="VIN75_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY8972585.1"
                     /translation="MRRVARTWATASAILLLSAGAAHAADTQSGNVEKCSRKLGVMAV
                     AEPHGGWGYLSAYQLGSPEQLLRMMVQQSGCFDVVERGIAMQNIQQERSFAESGELRG
                     ESNVGKGQMQAADFVMTPAVQIAANNTGGVGGALAGGLLNKFGLGGVAGGLKFKEAST
                     SLVIADVRSSIQVASAEGKATKTDFDIGGWGWTGAAFGAANGYTNTPEGKVIASSLLD
                     NWNHIVIAIRDNPSLIKTHSESGDVNAANSTRAGAPAKAGEILMARIANVKVYAGPSK
                     DSKILGTLSKTDELIASGDQKNGFVQVDSSNYSGWVQRSLVGPAH"
BASE COUNT          962 a         2421 c         2520 g         1150 t
ORIGIN      
        1 gcgctcgccg ccgtggccgg ctgaccgaga ccgcagcccc ggcctcggga aggcgaacgg
       61 gccggccgtc ccgcgacgac cggcccgctt cgttcgcgga cgcctcaggg acgcgtcatg
      121 cggcgagcag ctgccgcagc acgtacggca ggatgccgcc gtgcgcgtag tagtccacct
      181 cgatgggcgt gtcgatgcgc agcaccagcg gcaccttctg gctcttgccg tcggcgcggt
      241 ggatcgtgag cgtcgcctcg gattgcggcg tgatcggcgc cggccattcg atgtcgatgg
      301 tctcgttgcc ggtgatgccc aggctcgacc acgtgacgcc cgccttgaac tgcagcggca
      361 gcacgcccat gcccaccagg ttgttgcggt ggatgcgctc gaagctgcgc gcgaccacgg
      421 ccttgatgcc cagcaactgc gtgcccttgg cggcccagtc gcggctggag ccggtgccgt
      481 attcctcgcc ggcgaagacg acggtcggga tgccgtgcgc ctggtaggcc atcgccgcgt
      541 cgtagatcgg caccggcttg gagctgtcgg ccggcaggtc ggccgtcgcc gggccctggt
      601 agatcgtgta gccgccctcg acccggccgc cgtcggcgcg cgccggcagc atcaggttct
      661 tgatgcgcac gttggcgaag gtgccgcgca tcatcacctc gtggttgccg cggcgcgagc
      721 cgtacgagtt gaagtctgcc ggcagcacgc cgttggcctg caggtagcgg ccggcgggcg
      781 agtccgactt gatcgagccg gccggcgaga tgtggtcggt ggtgatcgag tcgccgaaca
      841 gcgccatgat gcgcgcgccc ttgaccgacg tcggcgccgc ctgcggcacc tgcgtgaacc
      901 cgtcgaagaa cggcggacgc gcgatgtagg tgctccgcgg ccagtcgtag acctgcccgg
      961 tgacgcccgt gatgtcgttc cacagcgcgc ccgggttgct cttgacctgg ccgtagttgg
     1021 cgcggaacga gtcggcgtcc atcgcgaact tcatcaggtc gtgcacctcc tgcgaggtcg
     1081 gccagatgtc gcccagccag acgtccttgc cgcccgtgcc cttgcccacc ggctcggtca
     1141 tcaggtcgac cttgacgttg ccggcgatgg cgtaggccag caccagcggc gggctggcca
     1201 ggaagttggc cttcaggttc gggtggatgc gtgcctcgaa gttgcgattg cccgacagca
     1261 cggccgccgc caccaggtcg ttctcgacga tggcctggtt gatctcgggc gtgaggtccc
     1321 cggcgttgcc gatgcaggtg gtgcagccgt aggccgccac gttgaagccc agttgctcga
     1381 gcgccggcag caggccggcc ttctgcaggt actcggtgac gatgcgcgag ccgggcgcca
     1441 gcgaggtctt gacgtgcggc ttgacgcgca gccccgcggc caccgccttc ttggccagca
     1501 ggccggcggc cagcatcacg ctcgggttgc tggtgttggt gcagctggtg atggccgcga
     1561 tcagcacgtc gccgtggccg atctcgacac cgctgggcgt gaccgcgcgc cgcgtgaccg
     1621 cctcgggcgg caggttgtag ccgttgctgg cggcgggcgt ggtcagcagc tcgtcgaact
     1681 tcctggacag gttgccgatc tcgatgcgat cctgcggccg cttcgggccg gccaggctgg
     1741 gcgcgacggt gcccaggtcg agcgtgagca cctcgctgta gtcgatcgag ccgctcgccg
     1801 gcgtgccgaa caggccctgg gcgcggaagt aggcctcgaa ggcctcgatc tcctcgttcg
     1861 tgcggccggt gccacggagg tactcgaccg tcttgtcgtc gaccgcgaag aagcccatcg
     1921 tggcgccgta ctcgggcgcc atgttggcca gcgtcgcgcg atcgggcacc gacagcgagg
     1981 ccgtgcccgc gccgtggaac tcgacgaact tgcccaccac cttggccttg cgcagctgct
     2041 cggtgacggt cagcaccagg tcggtggcgg tgacgccctc gcgcagctgg ccggtgaact
     2101 cgaagcccac gacgtcgggc gtcaggaagt agaccggctg gcccagcatc gcagcctcgg
     2161 cctcgatgcc gccgacgccc cagccgacca cgcccaggcc gttgatcatc gtggtgtggc
     2221 tgtcggtgcc caccagcgtg tcggggtagt agaccggcgg cttgccgtcg cccgtctcgt
     2281 gcttgtgcac gccgcgcgcc aggtactcga ggttgacctg gtggacgatg ccgaagcccg
     2341 ggggcacgac gccgaaggtg tcgaacgcct gcatgcccca cttcatgaac ttgtagcgct
     2401 cgttgttgcg ctggaactcg atcttcatgt tgatgtcgag cgagttcttg tgtgcgtagt
     2461 cgtcgatcat gacgctgtgg tcgacgacca ggtccaccgg caccagcggc tcgatggtct
     2521 tggggttctt cttctggttg gccgcctcgg tgcgcatggc ggccaggtcg cacagcagcg
     2581 gcacgccggt gaagtcctgc agcaccacgc gggcgacgat gaacgggatc tcctcgctgc
     2641 gctcgccggt gggcgcccag ctggcgagct ggcgcacgtg ctcgggggtg atgcgacggc
     2701 cgtcgcagtg gcgcaacacg gcctcgagca cgatgcggat cgacaccggc agccggttga
     2761 cctgcggcag ctcggcggcc agcgccggca gcgagtggaa gcggaacgac gtccccgagg
     2821 gcaacttgaa cgtctggagg gtgtgcgcga acggcgcggt ggcggccagg ggcatgtgtt
     2881 ctcctcgcgg tcggggcgac cggtgaaatg gcgacgggat gctgagccgg acatgttgcc
     2941 ccaaccggca tttccgctcc atgaccccgg gtgagtgtca ggtgtttttg gtcggattcg
     3001 ccgcgcaacg ccaacacgac ggcgggcgcc gtctcaaccg tcgaccttgc cgcgcccggc
     3061 cttgaccgcc gagcgcacgc tcttggcctc caggcggcgc cgctgggagc ccagcgtcgg
     3121 gcgggtcggg cggcgcttga cgggcggctt ctcgaccgag gcgaccagct cggccagccg
     3181 ctgcagcgcc tcggcgcggt tcatgtcctg gctgcggtgc gtctgcgcct tgatgacgac
     3241 cacgccgtcc tcggtgatgc ggtggtcgcg cagcgccagc agccgtgcct tgacgccctc
     3301 gggcagcgag gacgccttca cgtcgaaacg caggtgcacg gccgaggaca ccttgttcac
     3361 gttctgcccg ccggcgccct gggcgcggat cgccgtgatc tcgacttcgt ccgtggcaat
     3421 ggcgatgggc atgcaccgat tgtggcctgg cccaagcgct cgaccgacgc gcaccggccg
     3481 ggcctgcgct cagagcttgg cggctccgct gccgacggtc gcccagacgt tggcgccgtc
     3541 gaacgccagg tagttcgggc cggtgcccgg cgtcgggaaa ctgccgacgg ccacgccggt
     3601 ggacgcgagg atcttggtca ccgtcttgtc cgtgccgttg gccgtccaga tgtaagtccc
     3661 gtcgaagacg gcggtgtagg ccccgttgcc gatcggggtc gtgcccagga cggcgccatc
     3721 ggaagcccgc aggcgcgtgg cgacgttgcc gccgaccacc cagatgttgg tgccgtcgta
     3781 cgcaacgccc cgcgccgtgc cgacgctgta ggtgccgatc gtggcgcccg tggcctgcga
     3841 cagcttcgac acggtgccgg atgccgaatt gaccacccag acgttggtgc cgtcgaaggc
     3901 gacgctcaac ggattggcgc cgacgggata cgtgccgacc accgcaccgt tcgagacgcg
     3961 gatcttcgtg acgctcgcgc cgtagtagtt cgccgtccag atgtagttgc cgtcgtaggc
     4021 gatgcccgtc ggcgccgagc ccgtggcgta ggtgccctgc agcgcgccgt cgctggcgcg
     4081 gatcttggac acggagctgg ctgactggtt ggtcacccag atgttcgcgc cgtcgaaggc
     4141 gacgtccgtc ggaaagccgc ccgtcgcgta ggtgccgagc accgcgccgt ccgcagcacg
     4201 gatcttggag acggtgttgc ccgagaacga ggtgacccag acgtgcgcgc cgtcgaacgc
     4261 gatgcccagc gcgttggtca tcggcactgc ggccagcgac ccggtggacg gataccagcg
     4321 cagcaacgcc acctgcgcgg ggttgagggt ctgggcgaag ctcgccgagg cgacggcgga
     4381 cagggccgcg gcgagcgcgg cacgcttgat gacattcatt ggaaacctct ctgatgtttt
     4441 ggaactccgg cggcgccggt ctcgacgacc gccgtgccct gatttcaacc cgccagaggc
     4501 ccaccagatg agcgaccccg gaccggggtc gcgcgcgctc agtccggacg cgcggacggg
     4561 gcttcgcgac gcccgcggga gacgggcatg cgcaccagcg cgccgccatc gagcgcgcgc
     4621 gcccgcagct ggccgcagcc gccctcgacg tcctgccccg ccgactggcg cagcttggtc
     4681 agcacgccgc gcgaatgcag cgtgcgcgcg atggcggcgg ccttctcggg cgctgggcgc
     4741 gcgaagccca ggccctcgtt ggtgttgtag gggatcatgt tcatcaacgc gtacttgccc
     4801 ttgagcaggc gcacgatgcc ctcgatctcg tcatcgccgt cgttgacgcc ctccagcagc
     4861 gtccattggt actggatggg gtatccggtc gcgcgcgcgt agcgctcgcc cgcgtccacg
     4921 agttcctcgg gcgacatgcg cggcgcgcgc ggcagcagct cgcggcgcag cgcgtccctg
     4981 gtcgaatgca gcgacagggc cagcgccggc ttgacgggcc cctgcggcag gcgctcgaac
     5041 acgcgcggat cgccgacggt ggagaagacg agattcttgt ggccgatgcc gcccagcgtg
     5101 ccgagcaggt cgatcgcctc catcacgttg tccaggttgt gcgcgggctc gcccatgccc
     5161 atgaagacga ccttcctgac caggcgcctg gagcgcgcca gcgccacctg ggccacgatc
     5221 tcggcgctgc cgacctggcg cagcaggccg tcgcggccgg tcatgcagaa cacgcagccg
     5281 acggcgcatc ccacctgcgt ggacacgcac aggccgtcgc gcggcagcag cacgctctcg
     5341 accgtctggc cgtcgcacag gcccaccagc aggcgcgcgg aaccgtcctc gcccggatgc
     5401 tccgagcgca gcgtggccag gccggcgagt tcggtggcga gcgcgggcag cgcgtcgcgc
     5461 acggccagcg gcatgaagtc ggccaggcgc cgcttgccgg cgtcctgcgg ctgcgcgttg
     5521 acccactggc gcagcacgcg cgcctcgtgc tcgggcaagg caccgagagc acgcaggcga
     5581 aggcggaggt cgaggatgcg catggaacgg acggacggcg gaaagccgcc gattttccgt
     5641 caagtgcgcc cgcgcgtccc gcgcgcgtca cgcgcgggcg tgcgcaaagc ccgcatcagt
     5701 gggtcgccgc cgtcgcgccg caagccggcg caagggcaag cgccggcgca tcagtgcgcc
     5761 ggaccgacca gcgagcgctg cacccagccg ctgtagttgg acgaatcgac ctgcacgaag
     5821 ccgttcttct ggtcgccgct ggcgatcagt tcgtcggtct tggacagcgt gcccaggatc
     5881 ttgctgtcct tggacgggcc ggcgtagacc ttcacgttgg cgatgcgtgc catcaggatc
     5941 tcgcccgcct tggcgggagc gcccgcgcgc gtggagttgg cggcgttgac gtcgcccgac
     6001 tcgctgtgcg tcttgatcag cgacgggttg tcgcggatgg cgatgacgat gtggttccag
     6061 ttgtcgagca ggctggacgc gatgaccttg ccctcgggcg tgttggtgta gccgttggcc
     6121 gcgccgaagg cggcgcccgt ccagccccag ccgccgatgt cgaagtcggt cttggtggcc
     6181 ttgccttcgg ccgaggccac ctggatgctg gaacgcacgt cggcgatgac gaggctggtg
     6241 gacgcctcct tgaacttcag gccgccggcg acgccgccga ggccgaactt gttgagcagg
     6301 ccgcccgcga gcgcgccgcc aacgccgccg gtgttgttgg cggcgatctg caccgcgggc
     6361 gtcatcacga agtcggcggc ctgcatctgg cccttgccga cgttggactc gccgcgcagc
     6421 tcgccgcttt ccgcgaacga gcgctcctgc tggatgttct gcatcgcgat gccgcgctcg
     6481 acgacgtcga agcaccccga ttgctgcacc atcatgcgca gcagctgctc gggcgaaccg
     6541 agctggtagg ccgagaggta gccccagccg ccgtgcggct cggccacggc catcacgccc
     6601 agcttgcggc tgcacttctc gacgttgccg ctctgggtgt ccgcggcgtg cgcggcgccg
     6661 gccgacagca gcaggatggc ggacgcggtg gcccaggtcc gggcgactct tctcatggaa
     6721 actctcctcc agtggcgaac gcccgcttgc ggcgtcctga atggtggccg aaattgtgcc
     6781 gcccgccctg cgtggcgaca ctccctctgg agggggattt cgtggggatt gacgaatacg
     6841 gtcgagcaag ggttgcctcg atgggagcag ctacgggcta accccgagtt acatcgctcc
     6901 gccgctcgcc gccgcgtttt tttgtaccct tgcggcttgc ctttcgcggc ccgccgggcc
     6961 gtccgccgtt ccatgcgact ttccctcgcc tccgatcccg accgcgtcaa gcgctggacc
     7021 gtcgccgtgc tgctggcggg cctcgcgctg gcg
//
LOCUS       DATCRO010000006         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_855185, whole genome shotgun sequence.
ACCESSION   DATCRO010000006 DATCRO010000000
VERSION     DATCRO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855185"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..416)
                     /locus_tag="VIN75_00105"
     CDS             complement(18..416)
                     /locus_tag="VIN75_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8972586.1"
                     /translation="MHSLLHHPTLMDCRRLFLRNYEVMINIGVHDFEKKGEQRVLINV
                     DLYVPLAISTPRDDQLREVLDYDFIRSTIAQRMAKGHVHLQETLCDDVLALMLAHPNV
                     RAARVSTEKPDVYPDCDSVGVEVFGLKDAA"
     gene            complement(493..1308)
                     /locus_tag="VIN75_00110"
     CDS             complement(493..1308)
                     /locus_tag="VIN75_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8972587.1"
                     /translation="MATPHHPDTQISRPVALVTGAARRIGRVIALELAAAGFDIALHH
                     RGADARSSDDAEAAARELRDAGARVQLFAADFADPAATAALLPAIAGALGRVDAVVNS
                     ASRFEYDSPERFDSALLASLTRSNVAAPVELAQALAAQVRAQPAHARVTPCVVNLLDQ
                     KLHNPNPDYFSYTLTKAALGHATVLLAQALAPAVRVCGVSPGITLVSGPMDEDEFAKA
                     HKLTALGRSSTPEDIARAVRFLLESPAITGVDLAVDGGQHLVGQPRDVLFLVK"
     gene            1452..>2047
                     /locus_tag="VIN75_00115"
     CDS             1452..>2047
                     /locus_tag="VIN75_00115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8972588.1"
                     /translation="MAFDDFMAEALYAPGLGYYSGGRRIFGLGPGSGSDFVTAPELSP
                     FFGRALAVQVREALAASGTGRVYEFGAGSGALAEQLLEALDADDADDDGMLRVPVDLT
                     IIEVSGPLRERQQARLARFAPRVRWLDAWPAAIEGVVVGNEVLDAMPVKLLHRAGGRW
                     HERGVVDLADPASAARAAVGSNDGKHRFAFADLDAPFVL"
BASE COUNT          267 a          695 c          771 g          314 t
ORIGIN      
        1 cctgggttcc tcgaggctca tgctgcgtcc ttcaggccga acacttccac gcccaccgaa
       61 tcgcagtccg ggtagacgtc gggcttttcc gtcgacaccc gcgccgcccg cacgttcggg
      121 tgcgccagca tcagcgccag cacgtcgtcg cacagcgtct cctgcaggtg cacgtggccc
      181 ttggccatgc gctgggcgat cgtcgagcgg atgaagtcgt agtccagcac ttcgcgcagc
      241 tggtcgtcgc gcggcgtgga gatggccagc ggcacgtaca ggtcgacgtt gatcagcacg
      301 cgctgctcgc ccttcttctc gaagtcgtgc acgccgatgt tgatcatcac ctcgtagttg
      361 cgcaggaaca gccggcggca gtccatcagg gtggggtggt gcaggaggct gtgcatttca
      421 ggtgtctcaa aatggggtcg tcgggaagcg aggatcacat ccgggctccc ctcgggggcg
      481 gcgacggggc ggtcatttca cgaggaacag cacgtcgcgc ggctggccga cgaggtgctg
      541 gccgccgtcg acggccaggt cgacgccggt gatcgctggc gattcgagca ggaagcgcac
      601 ggcgcgcgcg atgtcctcgg gcgtggacga gcggccgagc gcggtgagct tgtgcgcctt
      661 ggcgaactcg tcctcgtcca tcgggccgga gacgagcgtg atgcccggcg agacgccgca
      721 cacgcgcacc gccggcgcca gcgcctgcgc gagcagcacg gtggcgtggc cgagcgcggc
      781 cttggtcagc gtgtacgaga agtagtcggg gttcgggttg tgcagcttct ggtcgaggag
      841 gttgacgacg cacggcgtca cgcgcgcatg cgccggctgc gcgcgcacct gcgccgcgag
      901 cgcctgcgcg agctcgaccg gcgcggcgac gttgctgcgg gtcagcgacg ccagcagtgc
      961 gctgtcgaag cgctcggggc tgtcgtactc gaagcgcgac gcgctgttga cgaccgcgtc
     1021 gacgcggccc agcgcgcccg cgatggccgg cagcagcgcg gcggtggccg ccgggtcggc
     1081 gaagtcggcc gcgaacagct ggacgcgcgc tccggcgtcg cgcagctccc gtgcggcggc
     1141 ctcggcgtcg tccgacgagc gggcgtcggc gccgcggtgg tgcagggcga tgtcgaagcc
     1201 ggcggcggcc agctcgagcg cgatgacgcg gccgatgcgg cgcgcggcgc cggtgacgag
     1261 cgccaccgga cgcgagattt gcgtgtcagg gtggtggggg gtggccatga tccctacaat
     1321 ttcattcgat gcagcacgat tccagtcccg gccttccggg cgccacgaca gattcctcga
     1381 ccgctccgac gacgcccggc gacgcgctgg tcgcccggat cgcgtcccgc atcgccgcag
     1441 ccggcggctg gatggcgttc gacgacttca tggcggaggc gctgtacgcg cccggcctcg
     1501 gctattacag cggcgggcgc cgcatcttcg gcctggggcc cggaagcggc agcgatttcg
     1561 tcaccgcccc cgaattatcg ccgttcttcg gccgcgcgct ggccgtgcag gtgcgcgagg
     1621 cgctggcggc ctcgggcacg gggcgggtct acgagttcgg cgccggctcg ggcgccctgg
     1681 ccgaacaact gctcgaggcg ctggacgccg acgacgccga cgacgacggc atgctgcgcg
     1741 tgccggtgga tctcacgatc atcgaggtct ccgggccgct tcgtgaacgc cagcaggcgc
     1801 gcctggcccg tttcgcgccg cgcgtgcgct ggctcgacgc ctggcctgct gcgatcgagg
     1861 gcgtggtggt cggcaacgag gtgctcgacg cgatgcccgt caagctgctg catcgcgccg
     1921 gcggccgctg gcacgagcgc ggcgtggtcg atctcgcgga tccggcgtcg gctgcccgcg
     1981 ccgcggtcgg atcgaacgac ggcaagcacc gcttcgcgtt cgcggacctg gacgccccgt
     2041 tcgtgct
//
LOCUS       DATCRO010000007        11443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_663910, whole genome shotgun sequence.
ACCESSION   DATCRO010000007 DATCRO010000000
VERSION     DATCRO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663910"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VIN75_00120"
     CDS             <1..698
                     /locus_tag="VIN75_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY8972589.1"
                     /translation="RVERVRHELKRRELTVKAVEALTPHFRRITLAGDSLSDFVSASF
                     DDHVKVFVGDARRDYTPRSFDNAARELVIEFALHGDGPAAEWAARAVPGHTLAIGGPK
                     GSLIVPLDYDWHLLAGDETAFPAVARRLEELPAGAKAIVVLKAADVADRRTFASAADV
                     SMTWVSTDAELLDAVRALALPEGDGYAWCAGEAACMAALRRELVEVKGHPGEATRAAA
                     YWKRGAQGHHENL"
     gene            complement(740..1603)
                     /locus_tag="VIN75_00125"
     CDS             complement(740..1603)
                     /locus_tag="VIN75_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972590.1"
                     /translation="MNDDDLWLDALLDDELDAAASLALQRRLAAEPALQRRLDDRRAL
                     RDVVRAQATRHSAPDELRANLMAMLPSRAAPGADTASSAHVDAPSNPRAALERRGSRS
                     AARRRVVLPWATGLGGALIASVLTLAVVAPGTLGWHRGEAMATLQTAEASEAVSAHTR
                     ALLVDHPIEVASSDQHTVRPWLSARLNFVPPVTDFAPQGFPLVGARRDVLSGETAAAL
                     VYRHGAHVISVFVRPVDPSATRAAEATRVVRGFNVIERTQGGLAFCFVSDANPHELAA
                     LADLVAPLAGN"
     gene            complement(1745..2281)
                     /locus_tag="VIN75_00130"
     CDS             complement(1745..2281)
                     /locus_tag="VIN75_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8972591.1"
                     /translation="MEDGQGRFAASVLPHLDAAYNLARWLVRDAHDAQDVVQDALVRA
                     LRHFDGFRGGDPRPWLLAIVRNAAFAWLGSRRSGEVDFADHEHELDAVLAMDAPPSDP
                     ESLAIRRAERREIDAAIAALPIAFREAVVLRELEELSYRDIARITDVPIGTVMSRLSR
                     ARHLLAAALRPEAARNLA"
     gene            complement(2290..2709)
                     /locus_tag="VIN75_00135"
     CDS             complement(2290..2709)
                     /locus_tag="VIN75_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8972592.1"
                     /translation="MSPHGHPKGEYRGARHGGSLMNMDRRLLLAGFAATLVAAASRAA
                     TDPEPRRIPVVARKFVFIPNEITVRQGESVVLEFTAPEVAMGFFAPELGLRALIVPGE
                     VARVPFTAGKAGRFDFLCDVFCGDGHEGMNGHLVVQA"
     gene            complement(2706..3599)
                     /locus_tag="VIN75_00140"
     CDS             complement(2706..3599)
                     /locus_tag="VIN75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8972593.1"
                     /translation="MPADFDRRDFLRLAGVGAGVVFASGLAGCAARAGAPSTDFHFVQ
                     LSDSHWGFEGPVVNPEARTTLRRAVAEVNALPMQPDFIVFTGDLTHTTDDPQERRKRL
                     REFKEIVADLKVRDIRFLPGEHDASLDKGAAFQEFFGPTHYSFDHRGVHFIALDNVSD
                     PAARVGDEQLAWLAKDLATLDKDAPVVVLTHRPLFDLAPQWDWATRDGDQVVAQLMPH
                     ANVTVFYGHIHQEHHHQTGHIAHHAATSLIFPLPAPGSQPNRTPIKWDASQPFRGLGF
                     RDVERQGAAEYAIAQHGVAGA"
     gene            3838..4227
                     /locus_tag="VIN75_00145"
     CDS             3838..4227
                     /locus_tag="VIN75_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972594.1"
                     /translation="MRFMLELADSELRGVARDGGLVRLRFSAAAVRDEAGERGWMSGV
                     ALEMSAASMQGDAAHLFGRIAEAGLTHDSRLLRRMDLPGTLTAAREGEVVSLALRLAN
                     GTPFIVTGHRLEALLADDARFAPDLSC"
     gene            4259..5032
                     /locus_tag="VIN75_00150"
     CDS             4259..5032
                     /locus_tag="VIN75_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8972595.1"
                     /translation="MTTTPIAGDVDYAAHGQGYGRQRRTDPRIEAWVHAALGDARTVL
                     NVGAGAGSYEPLDRHVLAIEPAAAMRAQRPAHLTPAVDGVAEKLPLDDQSFDAAMALV
                     TVHQWPDVARGLAELRRVTRGPIVLLTFDGDALDRYWMADYAPEMLAVERRRFPSIAS
                     LVEGLGGACEVRAIPIPNDCVDGFSEAFYARPEAFLDPAVRRSQSAWSFVPDDVQARI
                     VARLGSELASGQWDRKHGAWRTRPTFEGSLRGIVSRPPR"
     gene            complement(5040..5261)
                     /locus_tag="VIN75_00155"
     CDS             complement(5040..5261)
                     /locus_tag="VIN75_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972596.1"
                     /translation="MARPHPNPTTDDDRRPASATSQQELYGRRAPTDLDEANIGREPD
                     AIEDERPDPGRGEDASGQPSTARSGANTQ"
     gene            5362..5691
                     /locus_tag="VIN75_00160"
     CDS             5362..5691
                     /locus_tag="VIN75_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8972597.1"
                     /translation="MYSSTFIFAKRQFDDEFHRLDQQIAAAAKDLPGYLGEEAWENAA
                     TGLVSNVYYWSSLDALQQLMSHPAHLQAKAAQANWLAGYRVVISKVMRAYGDRDDLPG
                     VVAFETP"
     gene            complement(5714..6238)
                     /locus_tag="VIN75_00165"
     CDS             complement(5714..6238)
                     /locus_tag="VIN75_00165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8972598.1"
                     /translation="MSPTRPEREEDCSLPGPSPRPPEVPRATAVSGPAEVQARVDLLL
                     AQCRRRRGVLALLCVRVDAVARPGGLQVGAAMEQRVRHEVSNRIGNAVRASDAILRES
                     DRDTCVVLPGADASVAARVGERLRRMVNGDYRVGGELLQVRVRIGAAAHPEHGARAAE
                     LLRRAGDAGEAGDA"
     gene            6474..7028
                     /locus_tag="VIN75_00170"
     CDS             6474..7028
                     /locus_tag="VIN75_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY8972599.1"
                     /translation="MSDITSLLKAAADGDRQAADQAFALLYADLQRLAHSRMSRSGNF
                     TMLDTTALVHESWLRFQRAGNAVFEDQQHFMGYAARVMHSVVVDFVRSRRAARHGGDV
                     EHVTLNTAIGDSVSRQDDEILRVDEAMQQLAVADKRLHDVVELRYFGGLSEAEIAAIL
                     GVTERTVQRDWHKARLFLSSALNP"
     gene            7085..9799
                     /locus_tag="VIN75_00175"
     CDS             7085..9799
                     /locus_tag="VIN75_00175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8972600.1"
                     /translation="MSVGKETWGRLSPLLDELLDLPDDDRSARLAALSAEDPNIAQAV
                     AAMLEHLPAIERGEFMPSAAMPKPGGLAGQAIGPYTLVREIGHGGMGTVWLARRTDGR
                     YEGEVAIKFLRSGLFGHGDAARFQREGSILARLSHPNIARLLDAGVMADGTQPYLVLE
                     YIDGEPIDQYCQRLALSVEARLRLMLDVLAAVAQAHNRLILHRDLKPTNILVNKAGEV
                     KLLDFGIAKLLDDAGADQTALTARAGNAFTPEFAAPEQLQAGDVTTATDVYALGVLMY
                     ILLGGEHPTAAPTGAPLDRMRSVIETVPRRLSEAVLKRGGPTQRWSPESRRLAVEVRG
                     DVETIVAKALKKSPSERYANAGALADDIRRYLAHEPIAARPDSTLYRSLKFVQRHRTG
                     VAATSLAIAALGAGIGVALWQAREAQAQRVQAEGLIEYMIGDLRKKLQPVGRLDVLDG
                     VGLKALDYYASQDLDHLDADSLGRRARALHMIGDLAQQRGKFEEAARDFQQAADTTSK
                     LLKAHPNDPQRIFDQSQSEFYVGYIEWYRGRLHPAEAAFRRYLDMAQRMNKAKPGDHS
                     WQLEDVFAKTTVGTILTDLGRADEALPLQTQARAEIAQIVRSHPEDAVSYGNTIGWEV
                     ITLAALGRPEDAIQADKDKIAAAMGAPNADKDVDAQFLIANAHYEIAVWQRDLGRLAD
                     AKSTASLALDELNALNKREPANVNYITELVSAHGLLGFLLVDNGDRAGAREQLQLASE
                     GLASLLRRPTPKRTWRIGSAGRVAELRAQLATDDSERSAAGAALADYLADVRRYQADG
                     GEIIAQEQPVVARAELERGDLLARSGQGAEARDLWLAAASLMRPSAERLNPTAMTLLG
                     QLDLRLGRAQDARSWADRVLGTTYRHPAFADLQQRLGPTQQAGGAARP"
     gene            9815..10216
                     /locus_tag="VIN75_00180"
     CDS             9815..10216
                     /locus_tag="VIN75_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972601.1"
                     /translation="MSDTNPRVVPVAVNVSIETSGVKITCSPEPVPVAKGLDDVLITF
                     TLGGAAGFSFPVDDAIVADKNSKSDFPYKSWTISNTLAALYDRNNKAAKVKYTVNVVD
                     AQGTPYSLDPEIQNGGSAGTTCGDDDGDDDC"
     gene            complement(10225..>11443)
                     /locus_tag="VIN75_00185"
     CDS             complement(10225..>11443)
                     /locus_tag="VIN75_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8972602.1"
                     /translation="AWYYEAEPAPFLELVADAVWRSRLLEVSYRRWQAPEEVTRVLRP
                     LGLVLKAGIWYLAAQVATSDKGGVRTYRLSNILELEVTDERFERPADFDLGAWWQAST
                     RRFEKELATDTAQLRVTVAGMKALRDLGAAVAQAAEASAGEPDEAGWRTKVDGLTAPE
                     AQAMFLGGLPGPASELGLGEAMASAQLKLMAALPDGWREDARRVSARFHLDPIDWYRG
                     PSATDHLPAIAQAVWSEHRVAMRYESWKGEVSRRVDPLGLVLKAGIWYLAAQVGSGVR
                     TYRLSNILDLEVTDEGFERPADFDLGAWWLASTRRFEKELATDTAQLRVTAAGMKALR
                     DLGAAVAQAAEASAGEPDEAGWRRVTIPIESIPHAAAQVLRLGAQAEVLKPAALRRAV
                     VERLEAVKALYVD"
BASE COUNT         1714 a         4178 c         3989 g         1562 t
ORIGIN      
        1 gccgcgtcga acgcgtgcgc cacgaactca agcgccgcga gctgacggtg aaggccgtcg
       61 aggccctcac cccgcacttt cgtcgcatca cgctcgcggg cgattccctg tcggacttcg
      121 tcagcgcgtc cttcgacgac cacgtcaagg tcttcgtcgg cgacgcccgc cgcgactaca
      181 cgccgcgctc gttcgacaac gcggcgcgcg agctcgtgat cgagttcgcg ctgcacggcg
      241 acgggccggc cgccgaatgg gccgcacgcg cagtgcccgg ccacacgctg gccatcggcg
      301 ggccgaaggg ctcgctgatc gtgccgctgg actacgactg gcacctgctc gccggcgacg
      361 agacggcgtt ccccgcagtc gcgcgccgcc tggaggaact gccggcggga gccaaggcga
      421 tcgtggtgct caaggcggcc gatgtcgccg accgccgcac gttcgcgtcg gctgcggatg
      481 tatccatgac ctgggtctcg acggacgccg agctgctgga cgcggtgcgc gcgctggcgc
      541 tgccggaggg cgacggctac gcctggtgcg ccggcgaggc ggcgtgcatg gcggcgctgc
      601 ggcgcgagct ggtggaggtc aaggggcatc cgggcgaggc cactcgcgcg gcggcgtact
      661 ggaagcgggg cgcgcagggg catcacgaga atctgtgagg cgtgcggagg ggtctgggct
      721 cttccggccc gacgcgatct cagttccccg ccagcggcgc caccaggtcc gccaatgcgg
      781 caagttcgtg cggattcgcg tccgacacga agcagaacgc cagcccgccc tgcgtgcgct
      841 cgatcacgtt gaagccgcgc accacgcgcg tggcctcggc cgcgcgcgtg gccgacgggt
      901 cgacggggcg cacgaacacc gagatgacgt gggcgccgtg gcgatagacg agcgcggcgg
      961 cggtctcgcc ggacagcacg tcgcgccgcg cgccgaccag cggaaagccc tgcggcgcga
     1021 agtcggtcac cggcggcacg aagttcaggc gcgccgacag ccacggccgc acggtgtgct
     1081 ggtccgagct ggcgacctcg atcgggtgat cgaccagcaa cgcccgcgtg tgcgcgctca
     1141 ccgcctccga ggcctcggcc gtctgcagcg tggccatcgc ctcgccgcga tgccagccca
     1201 gggtgccggg cgccaccacc gccagcgtca gcacgctcgc gatcaatgcg ccgcccaggc
     1261 ccgtcgccca gggcagcacc acgcgccgac gggcagccga tcgcgagccg cggcgctcga
     1321 gcgcggcgcg cgggttcgac ggcgcgtcca cgtgcgccga cgaggccgtg tccgcgcccg
     1381 gcgcggcgcg cgatggcagc atcgccatca ggttggcgcg cagttcgtcg ggcgccgagt
     1441 gccgcgtcgc ctgcgcgcgg acgacgtcgc gcagcgcgcg ccggtcgtcc aggcgccgct
     1501 gcagcgccgg ctcggcggcc aggcggcgct gcaacgccag gctcgcggcg gcatcgagct
     1561 cgtcgtcgag cagcgcgtcc agccagagat cgtcgtcgtt catttgaaaa cctccgtccg
     1621 gcggactgcg gtgtgatcgc cggaggcgtg aacgcccttc ggaacggcca tgcgggcgct
     1681 cataaggcaa cctccgccct gcgggctgcg gtgcgagcgc ccttcggaac ggccgtgcgg
     1741 gcgctcatgc cagattcctc gcggcctcgg gccgcagcgc ggccgccagc aggtggcggg
     1801 cgcgcgaaag gcgcgacatc accgtgccga tcggcacgtc cgtgatgcgc gcgatgtcgc
     1861 ggtaggacag ctcctccagc tcgcgcagca cgaccgcctc gcggaacgcg atgggcagcg
     1921 ccgcgatggc cgcatcgatc tcccggcgct ccgcacgccg gatcgcgagc gactccggat
     1981 cgctgggcgg cgcgtccatg gccagcacgg cgtcgagctc gtgctcgtgg tcggcgaaat
     2041 cgacctcgcc cgatcgccga gagcccagcc acgcgaacgc cgcgttgcgc acgatggcca
     2101 gcagccacgg ccgcgggtcg ccgccgcgaa agccgtcgaa atggcgcagc gcgcgcacga
     2161 gcgcgtcctg cacgacgtcc tgcgcgtcgt gcgcgtcccg cacgagccag cgcgcgaggt
     2221 tgtaggccgc atccaggtgc ggcagcacgg acgccgcgaa gcgtccctgg ccgtcttcca
     2281 ttgccgtcct caagcctgga cgacgaggtg accgttcatc ccttcgtgtc cgtcgccgca
     2341 gaatacatcg cacaggaaat cgaaacgccc ggccttgccg gcggtgaacg gcaccctggc
     2401 cacctcgccc ggcacgatca gcgcgcgcag gcccagctcc ggcgcgaaga agcccatcgc
     2461 cacctctggc gcggtgaact cgagcacgac cgactcgccc tgccggaccg tgatctcgtt
     2521 ggggatgaac acgaacttgc gcgccaccac cgggatgcgg cgcggctcgg gatcggtcgc
     2581 ggcgcgcgag gcggccgcca ccagcgtggc cgcgaagccc gcgaggagaa gtcgtcgatc
     2641 catgttcatg agagaacctc cgtgccgcgc accgcggtat tcgcccttcg ggtggccgtg
     2701 cgggctcatg ctcccgcgac gccgtgctgc gcgatcgcgt actcggccgc gccctggcgc
     2761 tcgacgtcgc gaaacccgag gccgcggaac ggctgcgacg cgtcccactt gatcggcgtg
     2821 cggttcggct gcgagccggg cgcgggcagc gggaagatca gcgacgtggc cgcgtggtgc
     2881 gcgatgtggc ccgtctggtg gtggtgctcc tggtggatat ggccgtagaa caccgtcacg
     2941 ttcgcgtgcg gcatcagctg cgccaccacc tggtcgccgt cgcgcgtggc ccagtcccac
     3001 tgcggcgcaa ggtcgaacag cgggcggtgc gtcagcacga cgaccggcgc atccttgtcc
     3061 agcgtggcca ggtccttggc gagccaggcg agctgctcgt caccgacgcg cgcagccggg
     3121 tcggacacgt tgtccagcgc gatgaagtgc acgccgcggt ggtcgaagct gtagtgcgtc
     3181 gggccgaaga attcctggaa cgcggcgccc ttgtccagcg aggcgtcgtg ctcgccgggc
     3241 aggaagcgga tgtcgcgcac cttcaggtcg gccacgatct ccttgaactc gcgcaggcgc
     3301 ttgcggcgct cctgcgggtc gtcggtggtg tgcgtgaggt cgccggtgaa gacgatgaag
     3361 tcgggctgca tcggcagcgc gttgacctcg gccacggcgc gccggagcgt cgtcctggcc
     3421 tccgggttca cgaccgggcc ctcgaagccc cagtggctgt cggacagctg cacgaagtgg
     3481 aagtcggtcg acggcgcgcc ggcgcgcgcg gcgcaaccgg ccagtccgga cgcgaacacc
     3541 acgccggccc ccacgccggc caggcgcagg aagtcgcggc ggtcgaaatc ggcgggcatg
     3601 ggaactcctc gaagtggcgg gtatggctgc aagatcgggc ggggccgcgg tttattcccg
     3661 agggcctgaa gaatctcgtg cgaaggcgtg gccgcgccgg gggcgccgcc cgggccgaag
     3721 gagccggcgt tcctccctgc gtgccacaag ggtgcccatc gaggcggatg acgccttgcg
     3781 ggtgtcgcga cgagatgcaa gcggccgcct tgccgccctc gaggcagaat cgtctgcatg
     3841 cgcttcatgc ttgaactcgc cgattccgaa ctgcgcgggg tcgcgcgcga tggcgggctc
     3901 gttcgcctgc gattctcggc cgccgccgta cgcgacgagg ccggcgaacg cggctggatg
     3961 agcggcgtcg ccctggaaat gagcgccgcc tcgatgcaag gcgacgcggc ccacctgttc
     4021 ggcaggatcg ccgaagccgg gctgacgcac gactcgcgcc tcctgcgtcg catggacttg
     4081 cccggcaccc tgaccgccgc acgcgagggc gaggtcgtat ccctggctct gcgcctggcc
     4141 aacgggacac ccttcatcgt caccggccac cggctcgaag cgctgctcgc cgacgacgcc
     4201 cgcttcgcac ccgacctgtc gtgctgagcg cccgaatcca ccactcccga ccgcagacat
     4261 gaccaccacc cccatcgccg gcgatgtcga ctacgccgcc cacggccagg gctacggccg
     4321 ccagcgccgc accgacccgc gcatcgaggc ctgggtgcat gccgcgctcg gcgacgcccg
     4381 caccgtcctg aacgtcggcg cgggcgccgg ctcctacgaa ccgctggacc gccacgtgct
     4441 cgcgatcgag cccgcggccg cgatgcgcgc gcagcgcccg gcgcatctga cgccggcagt
     4501 ggacggcgtc gccgagaagc tgccgctgga cgaccagtcc ttcgacgccg cgatggcgct
     4561 ggtcaccgtt caccagtggc ccgacgtcgc caggggcctg gccgagctgc gccgcgtcac
     4621 gcgcgggccc atcgtgctgc tgaccttcga cggcgacgcg ctcgatcggt actggatggc
     4681 cgactacgcg ccggagatgc tggcggtcga gcgccgccgc ttccccagca tcgcgtcgct
     4741 ggtcgagggc ctgggcggcg cctgcgaggt gcgcgccatc ccgatcccga acgactgcgt
     4801 cgacggcttc tccgaggcgt tctacgctcg ccccgaggcc ttcctcgacc cggcggtgcg
     4861 gcgctcgcag tcggcgtgga gcttcgtgcc cgacgacgtg caggcgcgca tcgtggcgcg
     4921 cctgggcagc gagctcgcgt ccgggcaatg ggatcgcaag cacggcgcgt ggcgcacccg
     4981 gccgacgttc gaaggctcgc tgcgcggcat cgtcagccga ccgccgcgct gagccgcgct
     5041 cactgcgtgt tggcgccgct gcgcgcggtg ctgggctggc cggaggcgtc ctcgccgcgg
     5101 cccggatccg gccgctcgtc ctcgatcgcg tcgggctcgc ggccgatgtt ggcctcgtcc
     5161 aggtccgtcg gcgcgcggcg gccgtagagc tcctgctggc tggtcgccga ggcggggcgg
     5221 cggtcgtcgt cggtggtggg attgggatgc gggcgggcca tggtgcgctc ctgtcgggtg
     5281 tccgggcccc cggatcgggc aaggcccgcg cccgcgctat cctcggcggc cccaccccac
     5341 cttcttcgca ccgtcaccgt gatctacagc tcgaccttca tcttcgccaa gaggcagttc
     5401 gacgacgagt tccatcgtct cgaccagcag atcgccgccg ccgcgaagga cctgccgggg
     5461 tacctgggcg aggaggcgtg ggagaacgcg gccacgggcc tggtgtccaa cgtctactac
     5521 tggtcgtcgc tggacgcgct gcagcagctg atgtcgcacc cggcccacct gcaggccaag
     5581 gccgcgcagg ccaactggct ggcgggctac cgcgtggtca tctccaaggt gatgcgcgcg
     5641 tacggcgacc gcgacgacct gcccggcgtc gtcgcgttcg agacgcctta ggcagcttgg
     5701 ccgcgccggc cgctcaggcg tccccggcct cgccggcgtc ccccgcgcgc cgcagcagct
     5761 ccgccgcgcg cgcgccgtgc tcgggatgcg ccgcggcgcc gatgcgcacg cgcacctgca
     5821 gcagctcgcc gccgacgcgg tagtcgccgt tgaccatccg ccgcaggcgt tcgcccaccc
     5881 gcgcggcgac gctcgcgtcg gcgccgggca gcacgacgca ggtatcgcgg tcgctctcgc
     5941 gcaggatggc gtcgctcgcg cgcaccgcgt tgccgatccg gttcgatacc tcgtgccgta
     6001 cccgctgctc catggcggcg ccgacctgca ggccgcccgg acgcgccacg gcatcgacgc
     6061 gcacgcacag cagcgccagc acgccgcggc ggcgccggca ctgcgccagc aggagatcca
     6121 cgcgggcctg cacctcggcc ggccccgaga cagccgtcgc gcgcggcacc tcgggcgggc
     6181 gcggcgacgg gccgggcagg ctgcagtctt cctctcgttc ggggcgggtc ggcgacatca
     6241 caggcgttgt cctcgctgcg tggtgtcccg gcgacaactg gctgcgatcc gggacgtggt
     6301 gaggggcggc atcccggaca ggccctccgg cctcccttac ctaccaaaac actgcccctc
     6361 gccaaataga acgagaacag cgccacgctc ccgacacgag cagacgcaaa aatatgtaac
     6421 tacgcggtag agtaggcggt atgcgggtcc cgcggcccga aacgtaaaag cgcatgtccg
     6481 acatcacctc cttgctgaag gcggcggccg acggcgaccg ccaggcggcc gaccaggcgt
     6541 tcgcgctgct ctatgccgac ctgcagcgcc tggcgcacag ccgcatgagc cggtccggca
     6601 acttcacgat gctggacacc acggcgctgg tgcacgaaag ctggctgcgg ttccagcgcg
     6661 cgggcaacgc cgtgttcgag gaccagcagc acttcatggg gtatgcggcc cgggtgatgc
     6721 actcggtggt cgtcgacttc gtgcgctcgc ggcgcgcggc gcgccacggc ggcgacgtcg
     6781 agcacgtcac cctcaacacc gccatcggcg actccgtctc gcgccaggac gacgagatcc
     6841 tgcgcgtcga cgaggcgatg cagcagctcg cggtggcgga caagcggctg cacgacgtgg
     6901 tggaattgcg ctacttcggc ggactgtcgg aggccgagat cgccgcgatc ctcggcgtca
     6961 cggagcgcac ggtgcagcgc gactggcaca aggcgcggct gttcctttcc tcggcgttga
     7021 atccatagcg gcgcccggcc gcccgaagcc gcgagcatcg aagtgcgaag cacggagaca
     7081 tctgatgtct gttggaaaag agacctgggg ccgcctctcg ccgctcctgg acgaattgct
     7141 ggacctgccc gacgacgacc gcagcgcgcg cctggccgcc cttagcgccg aagacccgaa
     7201 catcgcgcag gccgtggcgg cgatgctgga gcacctgcct gcgatcgagc gcggcgagtt
     7261 catgccgtcc gcggccatgc ccaagcccgg cggcctcgcc ggccaggcca tcggcccgta
     7321 cacgctggtg cgcgagatcg gccacggcgg catgggcacc gtgtggctcg cgcggcgcac
     7381 cgacggccgc tacgagggcg aggtcgcgat caagttcctg cgctcgggcc tgttcggcca
     7441 cggcgacgcg gcgcgtttcc agcgcgaggg cagcatcctc gcgcgcctgt cgcacccgaa
     7501 catcgcgcgc ctgctggacg ccggtgtcat ggccgacggc acgcagccct acctggtgct
     7561 cgaatacatc gacggcgaac cgatcgacca gtactgccag cggctcgcgc tgtccgtcga
     7621 ggcgcgcctg cggctgatgc tggacgtgct cgcggccgtg gcgcaggcgc acaaccgcct
     7681 gatcctgcac cgcgacctca agcccaccaa catcctcgtc aacaaggcgg gcgaggtgaa
     7741 gctgctggac ttcggcatcg ccaagctgct ggacgacgcc ggcgccgacc agaccgcgct
     7801 caccgcgcgc gccggcaacg cgttcacgcc ggagttcgcg gcgcccgagc agctgcaggc
     7861 cggcgacgtg accacggcca ccgacgtgta cgcgctgggc gtgctgatgt acatcctgct
     7921 gggcggcgaa cacccgaccg ccgctcccac cggcgcgccg ctcgatcgca tgcgctcggt
     7981 gatcgagacc gtgcccaggc gcctgtccga ggcggtgctc aagcgcggcg gaccgacgca
     8041 gcgctggtcg ccggaatcgc gccggctcgc ggtcgaggtg cgcggcgacg tcgagaccat
     8101 cgtcgccaag gcgctcaaga agtcgccgtc cgaacgctat gccaacgcgg gcgcgctggc
     8161 cgacgacatc cgccgctacc tcgcgcacga gccgatcgcc gcgcggcccg actccacgct
     8221 gtaccgcagc ctgaagttcg tgcagcgcca ccgcaccggc gtggccgcca cgtcgctggc
     8281 catcgcggcg ctgggcgctg gcatcggcgt cgcgctgtgg caggcgcgcg aggcgcaggc
     8341 ccagcgcgtg caggccgagg ggctgatcga gtacatgatc ggcgacctgc gcaagaagct
     8401 gcagcccgtg gggcggctcg acgtgctgga cggcgtcggg ttgaaggcgc tggactacta
     8461 cgcttcgcag gatctcgacc atctcgatgc cgactcgctg gggcgccggg cgcgggcgct
     8521 gcacatgatc ggcgacctgg cgcagcagcg cgggaagttc gaggaggcgg cgcgcgattt
     8581 ccagcaggcg gccgatacca cgagcaagtt gctcaaggcg caccccaatg acccgcagcg
     8641 gatcttcgac cagtcgcaga gcgagttcta cgtcggctac atcgagtggt atcgaggacg
     8701 gctgcacccg gccgaggccg cgttccggcg ctacctcgac atggcgcaga ggatgaacaa
     8761 ggccaagccg ggggatcaca gctggcagtt ggaggacgtg ttcgccaaaa ccaccgtcgg
     8821 caccatcctc accgacctgg gccgcgccga cgaagcgctg ccactgcaga cgcaagcgcg
     8881 tgccgagatt gcgcagatcg tgcgcagcca tccggaagac gcggtcagct atggcaacac
     8941 catcggctgg gaagtgatca ccctggctgc gctgggccga cccgaggatg cgatccaggc
     9001 cgacaaggac aagatcgcag cggcgatggg cgcgcccaac gccgacaagg acgtcgatgc
     9061 gcaattcctc atcgccaacg cacattacga gattgctgtt tggcagcgcg acctaggtcg
     9121 tctggccgat gcaaagtcga ccgccagcct ggcactggac gaactgaacg cactgaacaa
     9181 gcgtgagcca gccaacgtca actacatcac cgagctggtc agtgcgcatg gcctgttggg
     9241 cttcttgctg gtcgacaacg gcgatcgggc cggcgcacgc gaacaactgc agttggccag
     9301 cgaaggccta gcgtcactgc tacggcggcc aaccccgaaa cgcacgtggc gcatcggctc
     9361 cgccggccgg gtcgccgaac tgcgagcgca gctggctacc gacgacagcg aaagaagcgc
     9421 ggccggcgcc gcgctggcgg actacctggc cgatgtgcgc cgttaccagg cagacggcgg
     9481 tgaaatcatc gcgcaggaac aacccgtggt cgcccgtgcc gaactcgagc gcggcgactt
     9541 gctcgctcga tccggccaag gcgcggaggc acgcgatctg tggctggccg cggcgtcgct
     9601 catgcgtccg tccgccgagc gcctgaaccc cacggcaatg actttgctcg gacaactcga
     9661 tcttcgcctg ggccgtgctc aagacgcacg gtcctgggcc gatagagtgc tgggcacgac
     9721 ctatcggcac cccgccttcg ccgatcttca acaacgactc ggcccgacgc agcaggcggg
     9781 gggagcggcc aggccatgat caagggaaac atccatgtct gacaccaacc cccgcgtcgt
     9841 tccggtcgcg gtcaacgttt cgatcgagac ttccggcgtc aagatcacct gcagtcccga
     9901 gccagtccct gtcgccaagg gtcttgacga cgtcctgatc acattcacgc tcgggggcgc
     9961 cgccggcttt agcttcccgg tcgacgacgc catcgtggcg gacaagaatt ccaagagcga
    10021 ctttccgtac aagtcctgga ccatcagcaa cacgctggct gcgctttacg accgcaacaa
    10081 caaggcagcc aaggtgaaat acaccgtgaa cgtggtcgat gcccaaggga ctccctacag
    10141 cttggacccc gagattcaga acggcggcag cgccggtacc acctgcggcg acgacgatgg
    10201 agacgacgac tgctgatcgt cacctcaatc gacgtagagc gccttcaccg cctctaaccg
    10261 ttccaccacc gcccgccgca acgccgcggg cttgagcacc tcggcctgcg cgccgaggcg
    10321 cagcacctgg gcggcggcgt gcgggatcga ttcgatcggg atcgtcacgc gccgccagcc
    10381 cgcctcatcc ggctcgccgg ccgaggcctc cgccgcctgc gccaccgcgg cgccgaggtc
    10441 gcgcaaggcc ttcatgcccg cggcagtcac ccgcagctgc gcggtgtccg tggccagctc
    10501 cttctcgaag cgcctcgtcg aggcgagcca ccacgcaccg aggtcgaagt ccgccgggcg
    10561 ctcgaagccc tcgtcggtga cctccagatc gaggatgttc gacagccggt aggtgcgcac
    10621 cccgctcccc acctgcgcgg ccaggtacca gatgcccgcc ttgagcacga gtcccagcgg
    10681 atcgacgcgg cgcgacacct cgcccttcca gctctcgtag cgcatcgcca cgcggtgctc
    10741 gctccacacg gcctgggcga tcgcgggcag gtggtcggtg gccgacgggc cgcggtacca
    10801 gtcgatcgga tccaggtgga agcgcgcgct gacccggcgc gcgtcctcgc gccagccgtc
    10861 gggcagcgcg gccatcagct tgagctgcgc cgaggccatc gcctcgccca ggcccagctc
    10921 cgatgccggc cccggcaggc cgcccaggaa catcgcctgg gcctcgggtg cggtcaggcc
    10981 gtcgaccttg gtgcgccagc ccgcctcgtc gggctcgccg gccgaggcct ccgccgcctg
    11041 cgccaccgcg gcgccgaggt cgcgcaaggc cttcatgccc gcgaccgtca cccgcagctg
    11101 cgcggtgtcc gtggccagct ccttctcgaa gcgccgcgtc gaggcctgcc accacgcgcc
    11161 caggtcgaag tccgccgggc gctcgaagcg ctcgtccgtg acctcgagct cgaggatgtt
    11221 cgacaagcgg taggtccgca cgccgccctt gtccgaggtc gccacctgcg cggccaggta
    11281 ccagatgccc gccttcagca cgagccccag cggccgcagc acgcgcgtca cttcctccgg
    11341 cgcctgccag cgccggtagg agacctccag caaccgcgag cgccacaccg cgtccgccac
    11401 gagctccagg aacggcgcgg gctccgcctc gtagtaccag gcg
//
LOCUS       DATCRO010000008         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_191368, whole genome shotgun sequence.
ACCESSION   DATCRO010000008 DATCRO010000000
VERSION     DATCRO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191368"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VIN75_00190"
     CDS             <1..774
                     /locus_tag="VIN75_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8972603.1"
                     /translation="HPDAPSLGVAEFVLHRGERIALVGGSGSGKSSLMRVLAGLYPAA
                     RIEIEVDGVVQPRAFDLASLATLIPQEADVFEGSVRENLDFGTGIDDARIAAAVHGSA
                     FGPVLDGLRGGLEFPVAQRGSNLSGGQRQRLCMARGALAAAGSSVVFLDEPTSALDPV
                     TEADVHQRLARTFPGACIVASVHRMSLLSYFDRVVLMDAGHVVDSGDVDALRERQPAF
                     ARMLQGSAHAAPAPAAQDPDVLQLLQALGAETDAETGAA"
     gene            complement(777..2036)
                     /locus_tag="VIN75_00195"
     CDS             complement(777..2036)
                     /locus_tag="VIN75_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8972604.1"
                     /translation="MQQDPLSQTRPTGRIHVLEVIGNAIVGGMETYVERLVEHLPPDR
                     FAITALCPFESSFTERLRARDIEVLVTPMPDDPPWSSIQMTCAMIKAGAIDVLHAHLP
                     NAHALAGIAGRLTGKPVLATLHGHRIGVLDLEIHRNAGTHLSVVCRQSYFHALGLGVS
                     AGQLSCNPNGVDTEAFQPRPRPADSLRAALGIPAAARIVGFLGRLSPEKGPEVFLRAA
                     LLAHARLPDTHFVLVGDGPLAPALRESIARMKLEGRVHLAGLRRDVAAVLNDMDVLVS
                     SSHSEAMPLAVMEAMASGLPVVATRVGGVPDMIAQGESGWLVAPNDFDEMATRLVQIL
                     ATPGELERMGRAARARAVDKMALSDTVDRMAQLLARLAPARTTQREISAVVSDASRRR
                     LPPARASAANGATAADGALPSPASRAD"
     gene            complement(2045..>2679)
                     /locus_tag="VIN75_00200"
     CDS             complement(2045..>2679)
                     /locus_tag="VIN75_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8972605.1"
                     /translation="YPESAVVQPTFAVASELKLPLLFHSGVFIDGRASRFCRPIGFEP
                     LRDHPGARVTLAHLGWPWTDEAIALGLIDRINGVREDDCAFRFDLSFGPPPPYRHEVL
                     DKALDVLGPGLLQFGSDCFLPCPGSAIAERRGWLESLLAEFDLDDAARDRIWHGTAAA
                     WLGLGAPAAPSRARGGGGVADDADHRARLHRVPASRRGWLGARPYSVRCC"
BASE COUNT          364 a          971 c          997 g          347 t
ORIGIN      
        1 cacccggacg cgccgtcgct gggcgtcgcc gagttcgtcc tccaccgcgg cgagcgcatc
       61 gccctcgtgg gcggcagcgg ctcgggcaag agctcgctga tgcgcgtgct cgccggcctg
      121 taccccgccg cgcggatcga gatcgaggtc gacggcgtcg tgcagccgcg cgcgttcgac
      181 ctcgcctcgc tggcgacgct gatcccgcag gaggccgatg tcttcgaggg ttccgtgcgc
      241 gagaacctgg acttcggcac cggcatcgac gacgcgcgca tcgccgcggc ggtgcacggc
      301 agcgcgttcg gccccgtgct ggacggcctg cgcggggggc tggagttccc ggtcgcccag
      361 cgcggcagca acctctcggg cggccagcgc cagcgcctgt gcatggcgcg cggcgcgctc
      421 gcggcggcgg gcagctcggt ggtgttcctc gacgagccca ccagcgcgct cgaccccgtc
      481 accgaggccg acgttcacca gcgactggcg cgcaccttcc ccggcgcgtg catcgtggcg
      541 tcggtccatc gcatgagcct gctgtcgtac ttcgaccgcg tcgtgctgat ggacgcgggc
      601 cacgtcgtcg actcgggcga cgtcgacgcg ttgcgcgaac ggcagcccgc cttcgcgcgc
      661 atgctccagg gcagcgcgca cgcggcgccc gcgcccgctg cgcaggatcc cgatgtgctg
      721 caactcctgc aggcgctcgg cgcggagacc gacgccgaga ccggcgccgc ctgagctcag
      781 tcggcgcgcg acgctgggga cggcagggcg ccgtccgctg ccgtcgcgcc gttggccgcg
      841 gacgcacgcg ccggcggcag gcggcggcgc gaggcgtcgc tgacgacggc gctgatctcg
      901 cgctgcgtcg tgcgcgccgg cgcgagccgc gccagcaact gcgccatgcg gtccaccgtg
      961 tcggacaggg ccatcttgtc gaccgctcgc gcgcgggccg cgcggcccat gcgctccagt
     1021 tcgcccggcg tcgcgaggat ctgcacgagg cgggtggcca tctcgtcgaa gtcgttgggc
     1081 gccaccagcc agccggactc gccctgggcg atcatgtcgg gcaccccgcc cacgcgcgtg
     1141 gccaccacgg gcagtccgct ggccatcgcc tccatcaccg ccagcggcat cgcctccgaa
     1201 tgcgagctcg agacgagcac gtccatgtcg ttgagcaccg ccgccacgtc gcgccgcaag
     1261 ccggccaggt gcacgcgacc ctcgagcttc atgcgcgcga tcgattccct gagcgccggc
     1321 gcgagcggcc cgtcgcccac cagcacgaag tgcgtgtcgg gcaagcgcgc gtgcgcgagc
     1381 aacgccgcgc gcaggaagac ctcggggccc ttctcgggcg acaaccgtcc gaggaagccg
     1441 acgatgcggg ccgccgcggg aatgccgagc gcggcacgca ggctgtccgc gggccgcggg
     1501 cgcggctgga aggcctcggt gtccacgccg ttgggattgc agctcagctg gccggcgctg
     1561 acgcccagtc ccagcgcgtg gaagtagctt tggcggcaga ccacgctcag gtgcgtgccg
     1621 gcgttgcgat ggatctccag atcgagcacg ccgatgcgat ggccgtgcag cgtcgcgagc
     1681 accggcttgc ccgtcaggcg ccccgcgatg ccggccagcg catgcgcgtt gggcagatgc
     1741 gcgtgcagca cgtcgatggc cccggccttg atcatcgcgc aggtcatctg gatcgacgac
     1801 cagggcgggt cgtcgggcat cggggtcacc agcacctcga tgtcgcgggc gcgcagccgc
     1861 tcggtgaacg agctctcgaa ggggcacagc gcggtgatcg cgaagcgatc gggcggcagg
     1921 tgttcgacca gccgctccac gtaggtctcc atcccgccga cgatggcgtt gccgatgact
     1981 tccagcacgt ggatgcggcc ggtcgggcgt gtctgtgaga gagggtcttg ttgcatggac
     2041 gggttcagca gcagcggacg gaatacgggc gcgcgccgag ccagccgcgc cggctcgccg
     2101 ggacgcgatg caaccgcgcg cgatggtcgg cgtcgtccgc gacaccgccg ccaccgcggg
     2161 cacgcgaggg tgcggcgggc gcacccaggc ccagccaggc cgccgcagtg ccgtgccaga
     2221 tgcggtcgcg cgccgcatcg tcgaggtcga attcggcaag cagcgattcg agccagccgc
     2281 gccgctcggc gatcgcactc ccggggcacg gcaggaagca gtcggagccg aactgcagca
     2341 ggccggggcc cagcacgtcg agcgccttgt ccagcacctc atgccggtag ggcggcggcg
     2401 gcccgaagga caggtcgaag cggaacgcgc agtcgtcctc gcgcacgccg ttgatgcggt
     2461 cgatcaggcc gagcgcgatc gcctcgtcgg tccaaggcca gcccaggtgc gcgagcgtca
     2521 cgcgcgcgcc cgggtggtcg cgcagcggct cgaagccgat cggccggcag aagcggctcg
     2581 cgcggccgtc gatgaacacg ccgctgtgga acagcagcgg gagcttcagt tcggaggcga
     2641 ccgcgaacgt gggctggacg acggcgctct cgggatacc
//
LOCUS       DATCRO010000009         7308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_618910, whole genome shotgun sequence.
ACCESSION   DATCRO010000009 DATCRO010000000
VERSION     DATCRO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618910"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..1035
                     /locus_tag="VIN75_00205"
     CDS             424..1035
                     /locus_tag="VIN75_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972606.1"
                     /translation="MAGTDPQRAPRDVELWLLARRAQGAALQLDFELRTAPARQVFRR
                     DFVYVSRQLHALEGSRRVQGLDRTRLNDALATLRQRADTIQAFMQQRAAELRAAVAVR
                     GPAGAHIAFARPARFQATIVSPGAHRFLGLVMQADDTLAVLEMAWLLGLVEPATRAAL
                     VSDCRRALLGFKDLACDLRHAVGLLVQEINAQRRAGHAAPSGD"
     gene            complement(1096..1986)
                     /locus_tag="VIN75_00210"
     CDS             complement(1096..1986)
                     /locus_tag="VIN75_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8972607.1"
                     /translation="MTPLDLDLVPEPPSSPAVMAARQRRALVVGLGGGRQGESGPGKP
                     PFAERRLRASDTVSTRFVDPLPGVDVAAWPRSGPRDGSSSVAPGASGDAPGSVRQGDA
                     AVSAAASAPASVSVSAATPGLASAFGSDGPSGPKDAAPDDGRDPADRRAAAIASAPGT
                     APATSPPSSTVAPPSPSANACGLRLDDALVVPAGALMRGYLRCHALLLVGDVVGNVHC
                     TAGPVVIRSGASLKGRLVATGDVYVCGTVGDSGGGAVITTRGKLTLAPGARVDGDVRS
                     GWLDVYDGAVLNGVGRRCED"
     gene            2875..3621
                     /locus_tag="VIN75_00215"
     CDS             2875..3621
                     /locus_tag="VIN75_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8972608.1"
                     /translation="MFIGTAAWSIPRAVADAFPGDGQHLERYARQFPCAEINSSFWRP
                     HQRATYARWAAMTPPHFRFAVKLPRAITHEARLRAARRPLRTFLDQATGLADRLGVLL
                     VQLPPSLAWDARVARTFFGLLRDHWDGPVACEPRHASWFDGPADRALARLRVTRVAAD
                     PARLAAAALPGGWLGPDGDGAGALQYWRWHGSPRPYWSSYDGDWLARQAAALARWPAQ
                     ATPWVIFDNTASGAAAANALALRSLVATRP"
     gene            complement(3632..4573)
                     /locus_tag="VIN75_00220"
     CDS             complement(3632..4573)
                     /locus_tag="VIN75_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8972609.1"
                     /translation="MTLAALRKTSLSVGACVVIGSALLVPVAASAFQADSSTGRSATE
                     PPVRGATGRQLWRLSDYTTIELVAREPGAPDNQHPYVAEANTLHAWLQQIQVVRGGAA
                     KPLFAIDELNNIIPSLVDALAHARPDQDVAVVSAARHEDNTFFSISAVTARLFAVDGH
                     LNIIVHDARYDFYDTARGSGMAPHFTVGSRTAEGSAPIQSPSATNKRLDWLVLGTAAP
                     APAAPTSPVAVPAPVAPPAPVAPPAPMAVPPVAPPAPIAPAAVPPAAPAPAMAPAAPA
                     AEQDAEVRLTKLKRLYEKGLITKSEYDKKRAEIIKGL"
     gene            complement(4681..5709)
                     /gene="murB"
                     /locus_tag="VIN75_00225"
     CDS             complement(4681..5709)
                     /gene="murB"
                     /locus_tag="VIN75_00225"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8972610.1"
                     /translation="MSSAPWLESDVPLKALNTFGLPATARRLARIRGDADVRRVLDSP
                     EVGRAAKLVLGGGSNLVLTRDVDEVVLKVEVDGLRLVETRADAFIVEAGAGMTWHDVV
                     TQTIARGWPGLENLALIPGTVGAAPVQNIGAYGLELADRFDSLDGVDLVTGLGITLDR
                     RQCLFGYRDSVFKNVLAGKAIITRVRLRLPRPWQPVLGYLELERKVAETGIAAPDARQ
                     VYAWICAIRRAKLPDPAVIGNAGSFFKNPIVTEDQCRDIIGRDPHVVHYPMADGTFKL
                     AAGWLIDACGWKGKSIGRAGVYEKQALVLVNRGNAIGAEVVTLAKAIQESVYGRFGIR
                     LEPEPVVL"
     gene            5767..6261
                     /locus_tag="VIN75_00230"
     CDS             5767..6261
                     /locus_tag="VIN75_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8972611.1"
                     /translation="MPSFDVLLEPEMPAIKNSVENTKKEIGTRFDFKGTSAAVELKEK
                     EKEIQLVGDGDFQIEQISTVLIGKLTKQNVPVTFLDMTAKIEKIGGDKVRQTYKIKAG
                     IDAPLAKKIVTAIKEAKLKVQGSIQGDTVRVTGKNRDDLQVGIALLRKEFPDQPLKYE
                     NFRD"
     gene            6379..>7308
                     /locus_tag="VIN75_00235"
     CDS             6379..>7308
                     /locus_tag="VIN75_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8972612.1"
                     /translation="MDLDFTPAQQAFRADVRAWVAANLPQDIAHKVHNGLRLHREDMQ
                     RWARILGAKGWLGYAWPKEFGGPGWDAIERHLFEEECALAGSPRIVPFGPVMVAPVIM
                     AFGSPEQQKKFLPGIASGDVWWSQGYSEPGSGSDLASVKTRAVRQGDKFIVNGQKTWT
                     TLGQYGEWIFCLVRTSTEGKPQTGISFLLIDMKSPGVTVRPIVLMDGEPEVNEVFFDN
                     VEVPAENLVGEENKGWTYAKYLLAHERTNIADVNRAKRELERLKRLAKQEGVWDDARF
                     RDQIALLEVDIVALEMMVLRVLSAQAGGKKALDI"
BASE COUNT         1071 a         2708 c         2578 g          951 t
ORIGIN      
        1 gccgcacccg ccgccacgcc tgaaacgccg cgtgcgctga tcacgctgcg gccttcgggt
       61 cgcatggccg cgccgcccgt gacggaccgc agcgccggtg atccggaccc gtcgcatcct
      121 ccggccacgg gcgacgctgc acccgccatg tccggccagc accatgatcc ggctggcccg
      181 cccagggacg caggcgcgac tcgcggggtc gtccacgccg gcccgacgga gggacccgac
      241 atcccgctag ccgcggcagg cgtccgggac gccgccgatg cgaaccctct cgccgagacc
      301 ccgagcggcc gcgacctcgc ggatgacggc ggcgccgcac cgcgcaccga tcgcgcaggc
      361 gaggccgtgc aggaaccgca ggccggatcc gcgctacccg acgagccgga ggaatcggcc
      421 gccatggcag gcaccgaccc gcaacgggcg ccgcgcgacg tggagctctg gctgctcgcg
      481 cggcgcgcac agggcgccgc gctgcagctg gacttcgagc tgcgcaccgc gcctgcccgc
      541 caggtgttcc ggcgcgactt cgtctacgtg tcgcgccagc tgcacgcgct cgaaggcagc
      601 cggcgcgtcc aaggcctgga ccgcacgcgc ctgaacgatg cgctcgcgac gctgcgccag
      661 cgcgccgaca cgatccaggc cttcatgcaa caacgcgccg ccgaactccg ggcggccgtg
      721 gccgtccgcg gcccggccgg cgcccacatc gccttcgcgc gcccggcccg cttccaggcg
      781 accatcgtga gccccggcgc gcaccgcttc ctcggcctgg tgatgcaggc cgacgacacg
      841 ctcgccgtcc tcgagatggc ctggttgctg ggcctggtcg aaccggccac ccgcgccgcg
      901 ctggtcagcg attgccgccg cgccctgctc ggtttcaagg acctcgcgtg cgacctgcgc
      961 cacgcggtgg gcctgctggt gcaggagatc aacgcgcagc ggcgggcggg ccatgcggcg
     1021 ccgtccggcg actagcggcg ggcggcgacc agcggcgggc ggcgactagc ggcgggcggc
     1081 ggcacggcgg cccgctcaat cctcgcagcg cctgcccacg ccattcagca cggcgccgtc
     1141 atacacatcc agccacccgc tgcgcacgtc cccgtccacg cgcgcaccgg gcgccagcgt
     1201 cagcttgccg cgcgtggtga tcacggcccc cccgccgctg tcgcccaccg tgccgcagac
     1261 gtagacgtcg ccggtcgcca ccagccgtcc cttcaggctg gcgccggagc ggatcaccac
     1321 ggggccggcg gtgcagtgga cattgccgac gacgtcgccg accagcagca acgcgtggca
     1381 gcgcaggtag cctcgcatca gggcgccggc ggggaccacc agcgcgtcgt ccagacgcag
     1441 tccgcaagca ttggctgagg gtgacggtgg cgcgaccgtg gacgacggcg ggctcgtcgc
     1501 cggcgcggtg cctggcgcgc tcgcgatggc cgcggcgcgc cggtctgcag gatcgcgccc
     1561 gtcgtccggc gccgcatcct tcggccccga aggcccatcg gacccgaatg ccgacgccag
     1621 acccggagtc gcagccgaca cggacaccga cgccggcgcc gacgctgccg ccgaaaccgc
     1681 cgcatctccc tggcgcacgg atcccggcgc atcgcccgac gcgcccggcg ccaccgacga
     1741 cgacccgtca cgcggaccac tccttggcca cgccgcgacg tccacccccg gcagcgggtc
     1801 cacgaagcgc gtggacaccg tgtccgacgc ccgcaaccgc cgctccgcga agggcggctt
     1861 gcccgggccg gactccccct ggcgtccgcc gcccagcccg accaccagcg cccgccgctg
     1921 gcgagcggcc atgaccgcgg gcgacgatgg cggctcggga acgagatcga ggtcgagcgg
     1981 cgtcatcggg aaccgtcctc gcggccgggc cgctccacgc gcggcgccga cgcgggcgcg
     2041 agcaccatcg ccacgcctga agacgccgag cctggcgggc gagcgaccgc ctgccctggc
     2101 gcgatcgtcg acgaaccgga cgacggtccc cgcccggccg gcgttcccca gacgccgccc
     2161 gccgtccccg cagccagccc ggccagtgaa cccgccagcg acacggcgac caggcccgcg
     2221 cggcgacgaa ccaccgaagc ggcgggcgtc gggcgagcgg aagatcgggc gttggccatg
     2281 gtgcgggttg cgtgacgtgt cgggtcgtga cgccatggtg ccccgacccg gcgccggccg
     2341 cccgcgtcct gacccccctc taaccggcca tttggcgcgg ggcgacctcg ccgatcccat
     2401 gccaagctgc ggatcgagcc gtccgcgacg cgaggtgcgc accggtgttg gcgcatcgac
     2461 cccgccttca ggccgtcatg cgcgcaggca ctcaactcct ggacgaatcg gccgatgaac
     2521 tcctccgtgt gcagaccgcg cacggatccg ccggcgtgcc ctgagtcctc cgactccggg
     2581 cacaacgttc gcacgcccgg cggatccacc gcatcgaaga tgccgcgccg gtgccgacga
     2641 cttcctccgt cggcgccgac gccggccctg cacgacgcca tcgcgccccc gccgggccgc
     2701 gatggcacat cgggtgactt ccccggcccc aacgccgggc gtcgcgacgg gtgacgccgt
     2761 caagcacgtc gtccgtcctt gcacgcctcc gaccgcggtc gggggcgttt tctttgggga
     2821 cgcccgggtg cggcgcttgc cgaaacggtg aatcgccgca agctctgcaa caccatgttc
     2881 atcggcaccg cggcctggag catcccgcgc gcggtcgcgg acgcgtttcc gggcgacggc
     2941 cagcatctcg agcgctatgc ccgccagttc ccgtgcgccg agatcaactc gtcgttctgg
     3001 cgcccgcacc agcgggccac ctacgcccgc tgggcggcca tgacgccgcc gcacttccgc
     3061 ttcgcggtga agctgccgcg cgcgatcacg cacgaggcca ggctgcgcgc cgcgcgccgg
     3121 ccgctgcgga ccttcctcga ccaggcgacc ggcctcgccg accggctggg cgtgctgctg
     3181 gtgcaactgc caccgtcgct ggcctgggac gcgcgggtcg cgcgcacctt cttcggcctg
     3241 ctgcgcgacc actgggacgg cccggtcgcc tgcgaaccgc gccatgcgag ctggttcgac
     3301 gggccggccg atcgcgcgct ggcgcgcctg cgcgtcacgc gcgtggcggc cgatccggcg
     3361 cgtcttgcgg cggcggcgct gcccggcggc tggctgggtc cggacggcga cggcgccggg
     3421 gcgctgcagt actggcgctg gcacggctcg ccgcggccct actggtcgag ctacgacggc
     3481 gactggctcg cgcgccaggc ggccgcgctc gcacgctggc ccgcgcaggc gacgccatgg
     3541 gtcatcttcg acaacacggc gtccggggcg gcggccgcca acgcgctggc gctgcggtcg
     3601 ctcgtcgcga cgcgtccgtg acgcgccggc ctcagagccc cttgatgatc tccgcccgct
     3661 tcttgtcgta ctcggacttg gtgatcaggc ccttctcgta caggcgcttg agcttggtca
     3721 ggcgcacttc cgcatcctgc tcggccgccg gggcggcggg cgccatggcg ggcgccggcg
     3781 cggccggcgg gacggccgcg ggtgcgatcg gtgccggcgg cgcgaccggc ggcacggcca
     3841 tcggggccgg cggcgccacg ggcgcgggcg gggcgaccgg cgcagggacg gcgaccggcg
     3901 aagtcggcgc ggccggcgcg ggcgcggccg tgcccagcac cagccagtcc aggcgcttgt
     3961 tcgtggcgct ggggctctgg atcggcgcgg agccctcggc cgtgcgcgag cccaccgtga
     4021 agtggggggc catgccgctg ccgcgggccg tgtcgtagaa gtcgtagcgc gcgtcgtgca
     4081 cgatgatgtt caggtggccg tccaccgcga acagccgcgc cgtcacggcc gagatgctga
     4141 agaacgtgtt gtcctcgtgg cgcgcagcgc tgacgaccgc cacgtcctgg tcggggcgcg
     4201 catgggccag cgcgtcgacc agggacggga tgatgttgtt gagctcgtcg atcgcgaaca
     4261 gcggcttggc ggcgccgccg cgcaccacct ggatctgctg cagccaggca tgcagcgtgt
     4321 tggcttcggc cacgtacggg tgctggttgt cgggcgcgcc gggttcgcgc gcgaccagct
     4381 cgatcgtcgt gtagtccgac aggcgccaca gctggcggcc ggtcgcgccg cgcaccgggg
     4441 gctcggtggc cgagcggccc gtcgacgagt cggcctggaa cgcgcttgcg gccacgggca
     4501 ccagcagcgc ggaaccgatg acgacgcagg cgccgaccga cagggaggtt ttgcgaaggg
     4561 ccgcgagcgt catcgagaga tctccagtgt tgttcgtggc ggcgccgtcc gggccggcat
     4621 gccgcgcacg cactctaaca aaggagaggc cgctgcgctg cacgcggccc ggcgaatcct
     4681 tcagaggacg accggctcgg gctccaggcg gatgccgaag cgcccgtaca cgctctcctg
     4741 gatcgccttg gccagcgtca ccacctcggc gccgatggcg ttgccgcggt tgaccagcac
     4801 cagcgcctgc ttctcgtaga cgcccgcgcg gccgatcgac ttgcccttcc agccgcacgc
     4861 gtcgatcagc cagcccgcgg cgagcttgaa cgtgccgtcg gccatcgggt agtgcaccac
     4921 gtgcgggtcg cgcccgatga tgtcgcggca ctggtcctcc gtgacgatcg ggttcttgaa
     4981 gaagctgccc gcgttgccga tgaccgccgg gtcgggcagt ttggcgcggc ggatggcgca
     5041 gatccaggcg tacacctgcc gggcgtccgg cgcggcgatg cccgtctcgg ccaccttgcg
     5101 ctcgagctcg aggtagccca gcaccggctg ccacggccgc ggcaggcgca agcgcacccg
     5161 cgtgatgatg gccttgccgg ccagcacgtt cttgaagacg ctgtcgcggt agccgaacag
     5221 gcattgccgc cggtcgagcg tgatgcccag gcccgtgacc aggtcgacgc cgtccagcga
     5281 gtcgaagcgg tcggccagct ccaggccgta cgcgccgatg ttctgcaccg gcgcggcgcc
     5341 caccgtgccc ggaatgagcg ccaggttctc cagccccggc cagccgcgcg cgatcgtctg
     5401 cgtgacgacg tcgtgccagg tcatgccggc gccggcttcc acgatgaacg cgtcggcgcg
     5461 cgtctccacc agccgcaggc cgtcgacctc gaccttgagc acgacctcgt cgacgtcgcg
     5521 cgtgaggacg aggttgctgc cgccgcccag cacgagcttg gcggcgcggc ccacctcggg
     5581 cgagtcgagc acgcggcgca cgtcggcatc gccgcggatg cgcgcaaggc gccgcgcggt
     5641 cgcgggcagg ccgaaggtgt tcaaggcctt caacgggaca tcggattcga gccagggggc
     5701 tgaggacatg gcagaatttt cgcaggtccc cgacctgtcc cctcacttcg ctccggattc
     5761 cctccaatgc ccagcttcga cgtcctcctc gaacccgaga tgcccgccat caagaactcg
     5821 gtcgaaaaca ccaagaagga gatcggcacc cgcttcgact tcaagggcac cagcgccgcc
     5881 gtcgagttga aggagaagga aaaggagatc cagctcgtcg gcgacggcga cttccagatc
     5941 gagcagatca gcaccgtgct gatcggcaag ctgaccaagc agaacgtgcc cgtcaccttc
     6001 ctggacatga ccgccaagat cgagaagatc ggcggtgaca aggtgcgcca gacctacaag
     6061 atcaaggccg gcatcgacgc cccgctcgcc aagaagatcg tcacggccat caaggaagcc
     6121 aagctcaagg tgcagggctc gatccagggc gacaccgtgc gcgtcaccgg caagaaccgc
     6181 gatgacctgc aggtgggcat cgcgctgctg cgcaaggaat ttccggatca gccgctcaag
     6241 tacgagaact tcagggattg agtccggcaa gcgggaaagc cccggtcgcc gtcgtcacct
     6301 cgacgacatc gaaattcgaa cgatcgtgct aaaacgcacc ctgcgccacc aaatccgaag
     6361 gcgcaggaga catcgtccat ggatctcgat ttcacaccgg cgcagcaggc cttccgggcc
     6421 gacgtccggg cctgggtggc ggcgaacctg ccgcaggaca tcgcgcacaa ggtgcacaac
     6481 ggcctgcgcc tgcatcgcga ggacatgcag cgctgggcgc gcatcctcgg ggccaagggc
     6541 tggctgggct atgcgtggcc caaggaattc ggcgggccgg ggtgggacgc catcgagcgc
     6601 cacctgttcg aggaggaatg cgcgctggcc gggtcgccgc gcatcgtgcc gttcgggccg
     6661 gtgatggtgg cgccggtgat catggcgttc ggctcgcccg aacagcagaa gaagttcctg
     6721 cccggcatcg cgtcgggcga cgtctggtgg agccagggct acagcgaacc gggttccggc
     6781 tccgacctcg cctcggtcaa gacgcgcgcc gtgcgccagg gcgacaagtt catcgtcaac
     6841 ggccagaaga cgtggacgac cctgggccag tacggcgagt ggatcttctg cctggtgcgc
     6901 acgtccaccg aaggcaagcc gcagacgggc atctcgttcc tgctgatcga catgaagtcg
     6961 ccgggcgtca ccgtgcgtcc catcgttctg atggacggcg agcccgaggt caacgaggtg
     7021 ttcttcgaca acgtcgaggt gcccgcggag aacctggtcg gcgaggagaa caagggctgg
     7081 acgtacgcca agtacctgct cgcgcacgag cgcaccaaca tcgccgacgt caaccgcgcc
     7141 aagcgcgagc tcgaacgcct gaagcgtctc gcgaagcagg aaggcgtgtg ggacgacgcg
     7201 cgcttccgcg accagatcgc gctgctggag gtcgacatcg tcgcgctcga gatgatggtg
     7261 ctgcgcgtgc tgagcgcgca ggccggcggc aagaaggcgc tggacatc
//
LOCUS       DATCRO010000010         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1057726, whole genome shotgun sequence.
ACCESSION   DATCRO010000010 DATCRO010000000
VERSION     DATCRO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057726"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2119)
                     /gene="rpoB"
                     /locus_tag="VIN75_00240"
     CDS             complement(<1..>2119)
                     /gene="rpoB"
                     /locus_tag="VIN75_00240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8972613.1"
                     /translation="YARIDRRRKIPVTSLMFALGLDGEAILSTFYKKILYKRTKEGRR
                     VPFDANRFRGYSTINDLIDADTGKVVLEAGKKLTVRAARQLQEKGLKALRLSDEELVG
                     NYLAEDLVNPKTGEIHAEAGEEITDKSMKALNEHGYKELPLLDIDHVNVGAYIRNTLS
                     ADKNMTREDALFDIYRVMRPGEPPTLDSAQAMFQSLFFDAERYDLSAVGRVKMNMRLD
                     LDAPDTQRTLRKEDILCVIKTLVDLRDGKGEIDDIDHLGNRRVRSVGELMENQYRIGL
                     LRMERAIKERMSSVDIDTVMPQDLINAKPAAAAVREFFGSSQLSQFMDQTNPLSEITH
                     KRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATFARVNKY
                     GFVETPYRKVKDGRVTDEVVYLSAMEEGRYSVAQANVPVDAKGRFTEDLVVCRASGTR
                     DVVPMTPDKVDYMDVSPKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLVRAEAP
                     FVGTGMEGVVARDSGAAIAARRSGVIDQIDATRVVIRATEDLDPTKSGVDIYRLMKYQ
                     RSNQSTCINQRPLVKVGDVVKKGDIIADGPSTDLGELALGRNVLVAFMPWNGYNFEDS
                     ILLSERIVKEDVFTSIHIEEFEVMARDTKLGPEEITRDIPNVSEEALKNLDEAGIVYI
                     GAEVRAGDILVGKITPKGESPMTP"
BASE COUNT          337 a          651 c          714 g          417 t
ORIGIN      
        1 ggcgtcatcg ggctttcgcc cttcggcgtg atcttgccga ccaggatgtc gccggcgcgc
       61 acttccgcgc cgatatagac gatgccggct tcgtcgaggt tcttcagcgc ttcttccgag
      121 acgttcggaa tgtcgcgggt gatttcctca ggcccgagct tggtgtcgcg ggccatcacc
      181 tcgaactcct cgatgtggat cgaggtgaag acgtcttcct tcacgatccg ctcggagagc
      241 aggatcgagt cttcgaagtt gtagccgttc cacggcatga acgcgacgag cacgttccgg
      301 ccgagcgcga gctcgccgag atcggtcgac ggaccgtcag cgatgatgtc gcccttcttg
      361 acgacgtcgc cgaccttcac cagcggacgc tggttgatgc aggtcgactg gttggagcgc
      421 tggtacttca tcaggcggta gatatcgacg cccgacttgg tcggatcgag atcttccgtg
      481 gcgcggatga cgacgcgggt cgcgtcgatc tggtcgatca cgcccgaacg gcgcgccgcg
      541 atcgcagcac ccgagtcgcg cgcaaccacg ccttccatgc cggtgccgac gaacggcgcc
      601 tcggcgcgaa ccagtggcac cgcctggcgc tgcatgttcg agcccatcag cgcgcggttg
      661 gcgtcgtcgt tctcgaggaa cgggatcagg gctgctgcga ccgaaacgag ctgcttcggc
      721 gaaacgtcca tgtagtcgac cttgtcgggc gtcatcggca cgacgtcgcg ggtaccgctg
      781 gcgcggcaga ccacgaggtc ttcggtgaaa cggcccttgg cgtcgacagg cacgttggcc
      841 tgcgcgaccg agtagcggcc ctcttccatc gccgagaggt acacgacctc gtcggtgacg
      901 cggccgtcct tgaccttgcg atacggcgtc tcgacgaagc cgtacttgtt cacgcgcgcg
      961 aaggtggcga gcgagttgat cagaccgatg ttcggacctt ccggcgtctc gatcgggcag
     1021 atacggccgt aatgcgtcgg atgcacgtcg cgcacctcga agccggcgcg ctcgcgggtc
     1081 agaccgcccg gtccaagcgc cgagaggcgg cgcttgtggg tgatttcgct gagcgggttg
     1141 gtctggtcca tgaactgcga cagctgcgag gagccgaaga attcgcgcac ggcggcagcc
     1201 gccggcttgg cgttgatcag gtcctgcggc atgaccgtgt cgatgtcgac cgaggacatg
     1261 cgctccttga tcgcgcgctc catgcgcagc aggccgatgc ggtactggtt ctccatgagc
     1321 tcgccgaccg agcgcacacg gcggttgccg agatggtcga tgtcgtcgat ctcgcccttg
     1381 ccgtcgcgca ggtccaccag cgtcttgatg acgcagagga tgtcttcctt gcgcagcgtg
     1441 cgctgggtat cgggcgcatc gagatcgagg cgcatgttca tcttgacgcg gccgaccgcg
     1501 gagaggtcgt aacgctcggc gtcgaagaac agcgactgga acatcgcctg cgccgaatcc
     1561 agcgtcggcg gctcgccggg gcgcatcacg cggtagatgt cgaacagcgc gtcttcacgc
     1621 gtcatgttct tatccgccga gagcgtgttg cggatgtagg cgccgacatt gacgtggtcg
     1681 atgtcgagca gcggcagttc cttgtagccg tgctcgttga gggccttcat cgacttgtcg
     1741 gtgatttcct cgccggcttc ggcgtggatc tcaccggtct tcgggttgac gaggtcctcg
     1801 gcgaggtagt tgccgacgag ttcctcatcc gacagacgca gcgctttgag ccccttctcc
     1861 tggagctggc gggcggcgcg gacggtgagc ttcttgccgg cctcgagcac gaccttgccg
     1921 gtatcggcgt cgatcaggtc gttgatggtc gagtagccgc ggaaacggtt ggcgtcgaac
     1981 ggaacgcgcc tgccttcctt ggtccgcttg tagagaatct tcttgtagaa cgtggacagg
     2041 atcgcctcgc cgtcgaggcc gagggcgaac atcagcgacg tcaccggaat cttgcggcga
     2101 cggtcgatac gcgcataga
//
LOCUS       DATCRO010000011         8231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_213897, whole genome shotgun sequence.
ACCESSION   DATCRO010000011 DATCRO010000000
VERSION     DATCRO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213897"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VIN75_00245"
     CDS             complement(<1..1014)
                     /locus_tag="VIN75_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972614.1"
                     /translation="MRLLPRPASTSPRAPRRRRAGWVALAVLAALVAVGLAFDANWLR
                     PLIRHHVMARSGRSVQFDDLKLHFDDGLDPTFEFRGLVIQNAPWAASRAPLIRAGRLA
                     ATVSWRTLGSGMTVVRRLELDDAQVDMERQADGLRNWRITRPDDRGPPRVRVLELDAR
                     NSSLHLVHAGIPLVLDARSTPLPAPHVLPAHADLPLTRHLDVAGRYRDRAFAGQADVS
                     DVLTFGATPRVFALRAQARLGALSVQAAGTSNDVHALGDVDVDASLATAGPGDVWPLP
                     ELLARLRPLSAQGHVVHAGERWTGSGVRMRGGRGTSVVVDASFTGGFRSDTPRRALRA
                     TLRD"
     gene            1415..2719
                     /locus_tag="VIN75_00250"
     CDS             1415..2719
                     /locus_tag="VIN75_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY8972615.1"
                     /translation="MRFLFRYFLAALLSPLAASAQDAESWNAHFQSTYIRQFQPAFHS
                     PYAGPRSLRAQPGASYSLTATAALGVRLGDGTEAYLDPEVVQGVPLSGLGGLAAFPNG
                     ELAKTSGRDPTLYLARLFVRHTFELGGDAVSVDADANQLKSTYAARRVVVTAGRLSLL
                     DVFDTSSYAHDPRTQFLNWALMTHGAFDYPADARGYTDGLAIDWDDGPWTLRAGRFAE
                     PKLPNGLELDGDLLRHHGDQVELVHTHAFGALAGAVRVIAFRARARMGAYDDALAAAP
                     VGTAPSLEAVRGADHAKSGFGIDAEQQLAGDLGVFVRALRADGRTETEAFTEADASVS
                     AGLSLKGVRWSRAKDVVGLAAGAQAISAGHRDYLARGGITDFLGDGALRYGHEQFAEL
                     YYAAELNAGFTLTADVQRVANPGYNKDRGPVSFFALRLHWEV"
     gene            2721..4019
                     /locus_tag="VIN75_00255"
     CDS             2721..4019
                     /locus_tag="VIN75_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY8972616.1"
                     /translation="MNVKLNGRIDGFARRLGPGLITGAADDDPSGIATYSQIGSQFRF
                     GLVWTLLLTTPLMIGIQMLSARIGWVTGEGLGANIAKMCPRWLTLALVGLLVAANTIN
                     IAADIGAMGEAVRLLAGGHASVYVAGFGALSIVGQVAFSYERTVRVLKWLTLGLFAYV
                     AVILSVSVPWRQVAVEGLAPWRFVPAGTTMTDYASMVVAVLGTTISPYLFFWQASQEV
                     EDNARRPEAAKLRDNPEYAAEHLSRIKQDTSFGMVFSNVIALCIVIATGVTLNLHGVT
                     NIQTSSQAAEALRPVAGQFAFAIFALGIIGTGLLAVPVLAGSAAYAVSEAFGWKSGLS
                     RGFHEARGFYVIIIAATLIGTLMSTLEVDPIKALVWSAIVNGVISVPIMVVMMWIGQS
                     RRLMGRFTISLRHRIFGWAATAVMGVAVAGLFFTSWRAGQ"
     gene            complement(4021..5115)
                     /locus_tag="VIN75_00260"
     CDS             complement(4021..5115)
                     /locus_tag="VIN75_00260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8972617.1"
                     /translation="MNSKQPQLQAQRVKTTTMTVVSSLLQALLIGLFAWNGTVGWTVA
                     GWFFVLSVGSTGFFLLSVVRGWNLRFRDRWLLWAQLISNYVIQLIFIVAAPRLWMIFL
                     ASSLVSFNYAMLGFTPQQFRWTWLGFGATTALALWIGRAQFAYPELTNLNIALMWLFF
                     FLAVRRLSLIGMQFSTLRAELSQRNKQLTLSLARIQELASHDDLTGAFNRRHFMELLQ
                     DERERSHRTQQPYSVALFDLDHFKTINDRFGHAAGDAVLRDFCTLVQAHMRVTDRFAR
                     WGGEEFVLLMPVTTPVESASLAVERIRSAVAAHAWTAIDALPADFRVTVSAGVATSVQ
                     GEPVEALVARADTALYQAKRAGRNCFVMAP"
     gene            complement(5274..5642)
                     /locus_tag="VIN75_00265"
     CDS             complement(5274..5642)
                     /locus_tag="VIN75_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972618.1"
                     /translation="MQHIVVGTFDQQAAACAAIVALANDRFDPRRVHAIETPVPSTAE
                     AADSAPAAPVADDSVVDHLRNFFAGLLGIGHDVSAYAAAMRRGGAVVRVDVDDQPQAA
                     RAELAFARAGAIDITRRPRH"
     gene            complement(5710..6252)
                     /locus_tag="VIN75_00270"
     CDS             complement(5710..6252)
                     /locus_tag="VIN75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY8972619.1"
                     /translation="MQSKPLTLLSAATVALAAALGAHAADTSAKLSHQDKSFIKDAAE
                     AGNAEVEGSKVAVSQSANGDVKAFAQQMVDDHGKAGSELKALADQKGVKVSDEPSTLK
                     KTEIKMLSGRKGSSFDQHYADSIGVKAHEDTVKLFQKEIDKGTDADVKAWAQKTLPTL
                     QHHLEMARQLKTKTDAEPKS"
     gene            6371..7438
                     /locus_tag="VIN75_00275"
     CDS             6371..7438
                     /locus_tag="VIN75_00275"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8972620.1"
                     /translation="MNTAAARSFTARLGLELPIVQAPLAGFQGVAMAAAVAGAGALGS
                     LPGAAMTPQALRDAVAEIRRGGRLPVNLNFFCHVVPPADPQREAAWREALRPYYDELG
                     VPESAIQNGPGRLPFGEEAAAAIEALRPEVVSFHFGLPPAPLLERVRATGAQVWSSAT
                     TVAEAQWLAAHGVDAVIAQGADAGGHRAVFLDYGPACDLSTQLRTLPLLTSILEVVRV
                     PVIAAGGISTAEGVAAALRLGASAVQLGTAFLLCPEATTSAVHRAALQGEGARDTALT
                     NLFTGRPARGIVNRLMREMGPMSTLPPAFPSAVNALAPLRAAAEQAGRGDFSPLWAGQ
                     DVSGCRAIPAAQLVRELAAKL"
     gene            complement(7435..7875)
                     /locus_tag="VIN75_00280"
     CDS             complement(7435..7875)
                     /locus_tag="VIN75_00280"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY8972621.1"
                     /translation="MLSAAPAPVDEATLADLVARARAVLPNAHAPYSDFRVAAAVLDE
                     QGRVHLGVNVENASYGLTNCAERVAIGVAVAAGAKRILAVGVTAAKLHPITPCGACRQ
                     VIREFAGADAPVASDAADGSLVITTAGALLPGAFSFETDPDGPG"
     gene            complement(7869..8057)
                     /locus_tag="VIN75_00285"
     CDS             complement(7869..8057)
                     /locus_tag="VIN75_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972622.1"
                     /translation="MTIDLPATHPAATEHHEEIVQFVFRSAASSGLLEHARALLGAAP
                     KELAESFEQAVRTSGLAS"
     gene            complement(8047..>8231)
                     /locus_tag="VIN75_00290"
     CDS             complement(8047..>8231)
                     /locus_tag="VIN75_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972623.1"
                     /translation="TKARDIVGVLNVDGLDSIPPLLQTPGDPTLKTLVFTLWASTEKI
                     RESLALADTGEPLHDD"
BASE COUNT         1099 a         2962 c         2990 g         1180 t
ORIGIN      
        1 gtcgcgcagc gtggcccgca gcgcgcggcg cggcgtatcg ctcctgaagc cgccggtgaa
       61 gctcgcgtcg acgaccacgc tcgtgccgcg gcccccgcgc atccgcacgc ccgaccccgt
      121 ccagcgctcc cccgcgtgca ccacgtggcc ctgcgccgac agcggccgca gccgcgccag
      181 cagctcgggc aggggccaga cgtcgcccgg gccggccgtg gcgagggacg cgtcgacgtc
      241 gacgtcgccc agcgcatgca cgtcgttgct ggtgcccgcg gcctgcacgg acagcgcgcc
      301 caggcgggcc tgcgcgcgca gcgcgaacac gcgcggcgtg gcgccgaagg tcagcacgtc
      361 gctgacgtcg gcctggcccg cgaacgcgcg atcgcggtag cggccggcga cgtcgaggtg
      421 gcgcgtgagc ggcaggtcgg catgcgcggg cagcacgtgc ggtgcgggca gcggcgtcga
      481 gcgcgcgtcc agcaccagcg ggatgccggc gtgcaccagg tgcagcgagc tgttgcgggc
      541 atcgagctcg agcacgcgga cgcggggcgg gccgcggtcg tcgggccgcg tgatgcgcca
      601 gttgcgcagg ccgtcggcct ggcgctccat gtcgacctgc gcgtcgtcga gctccaggcg
      661 gcgcacgacc gtcatgcccg agccgagcgt gcgccacgac acggtcgccg cgaggcgccc
      721 cgcgcggatc agcggcgccc tggacgccgc ccagggcgcg ttctggatca cgaggccgcg
      781 gaactcgaac gtgggatcga gcccatcgtc gaaatgcagc ttcaggtcgt cgaactgcac
      841 gctgcggccc gagcgcgcca tcacgtggtg ccggatcagc ggtcgcagcc agttcgcgtc
      901 gaacgcgaga ccgaccgcga cgagcgccgc gagcaccgcg agcgccaccc agcccgcgcg
      961 ccggcggcgc ggcgcgcgcg gggacgtcga tgcgggacgg ggcagcaggc gcatcccgcg
     1021 gcagagcatg cggcgtgccc tgtaggcgaa ccggagattc aggcgcggcg ctgtccgacg
     1081 ccggccgtcg cctgaacgcc cgcttgcgct ggccgtcgcg tgcatgaacc ggcttgaccc
     1141 gacggcgacg gcctgccgat aatcgccgct tctcccacga cacgcaccga ccatggactg
     1201 ccatcccagt cgcgtgcctt cgtcgtccgc ggcgcgcgcc tgatcacccg ggctcgcgac
     1261 gggcggcaga aggcacccac gccctgaccg cccggcaagc aagccacctc cgcgacgcgc
     1321 ccgcgtccgc cgcgcttcct tccggttctc cccgatccga cgcccaccgg cgtccatccg
     1381 ccagcgcctc gaacggcgcg cggaggagtt ccgcatgcga ttcctgttcc gatacttcct
     1441 cgcggccctg ctgtcgcccc ttgccgcgtc cgcccaggac gcggagtcct ggaacgccca
     1501 tttccagtcg acctacatcc gccagttcca gcccgccttc cattcgccgt atgctggccc
     1561 gcgcagcctg cgcgcgcaac cgggcgcgag ctattcgctg accgccaccg cggcgctggg
     1621 cgtgcgcctg ggcgacggca ccgaggccta cctcgatccc gaggtcgtgc agggcgtgcc
     1681 gctgtcgggc ctcggcggcc tggccgcgtt ccccaacggc gagctcgcca agacctcggg
     1741 ccgcgacccg acgctctacc tcgcgcgcct gttcgttcgc cacaccttcg agctgggagg
     1801 cgatgcggtg tccgtcgacg ccgacgccaa ccagctcaag tcgacctacg cggcgcggcg
     1861 tgtcgtcgtc accgcggggc gcctgtccct gctggacgtg ttcgacacca gctcgtatgc
     1921 ccacgacccg cgcacgcagt tcctgaactg ggcgctgatg acgcacggcg ccttcgacta
     1981 cccggccgac gcgcgcggct acaccgacgg cctcgcgatc gactgggacg acggcccgtg
     2041 gacgctgcgc gccggccgct tcgccgagcc gaagctgccc aacgggctcg aactggatgg
     2101 cgacctgctg cgccaccacg gcgaccaggt ggagctcgtg cacacgcatg cgttcggcgc
     2161 gctcgccggg gcggtgcgcg tgatcgcgtt ccgcgcccgc gcgcgcatgg gcgcctacga
     2221 cgatgcgctg gccgctgcgc ccgtcggcac cgcgccgagc ctggaggccg tgcgcggcgc
     2281 cgatcacgcc aagtccggct tcggcatcga cgccgaacag cagctggccg gcgacctggg
     2341 cgtgttcgtg cgggcgctgc gcgccgacgg ccgcaccgag accgaggcgt tcacggaggc
     2401 cgatgcgtcg gtcagcgccg gcctgtcgct caagggcgtg cgctggtcgc gcgcgaagga
     2461 cgtcgtcggg ctggcggccg gcgcgcaggc gatttccgcc gggcaccgcg actacctcgc
     2521 ccgcggcggc atcacggatt tcctcggcga cggcgcgctg cgctacggac acgagcagtt
     2581 cgccgagctg tactacgcgg cggaactgaa cgccggcttc acgctcacag ccgatgtgca
     2641 gcgggtcgcc aatccgggct acaacaagga tcgcgggccg gtgtcgttct tcgcgctgcg
     2701 cctgcactgg gaggtgtgac atgaacgtca agctcaacgg ccgcatcgac ggcttcgcca
     2761 ggcggctcgg ccccggcctc atcacgggcg ccgcggacga cgatccgagc ggcatcgcca
     2821 cctattcgca gatcggctcg cagttccgct tcggcctcgt gtggacgctg ctgctgacca
     2881 cgccgctgat gatcggcatc cagatgctgt cggcacgcat cggctgggtc accggcgagg
     2941 gcctgggcgc caacatcgcc aagatgtgcc cgcgctggct gacgctggcg ctcgtggggc
     3001 tgctggtggc ggccaacacc atcaacatcg ccgccgacat cggcgcgatg ggcgaggccg
     3061 tgcgcctgct cgcgggcggc cacgcgagcg tctacgtggc gggcttcggc gcgctgtcga
     3121 tcgtcggcca ggtggcgttc tcgtacgagc gcacggtgcg cgtgctcaag tggctcacgc
     3181 tggggctgtt cgcctacgtg gcggtgatcc tgtcggtgtc ggtgccgtgg cgccaggtgg
     3241 ccgtcgaggg cctggcgccg tggcgcttcg tgcccgcggg cacgacgatg accgactacg
     3301 cgtccatggt ggtcgccgtg ctcggcacca cgatcagccc ctacctgttc ttctggcagg
     3361 cctcgcagga ggtggaggac aacgcgcgcc ggcccgaggc cgcgaagttg cgcgacaacc
     3421 cggaatacgc cgcggagcac ctgtcgcgca tcaagcagga cacgtcgttc ggcatggtgt
     3481 tctccaacgt gatcgcgttg tgcatcgtca tcgccacggg cgtcacgctg aacctgcacg
     3541 gggtcaccaa catccagacc tcgtcgcagg ccgccgaggc gctgcggccg gtggccgggc
     3601 agttcgcgtt cgcgatcttc gcgctgggca tcatcggcac cgggctgctg gccgtgccgg
     3661 tgctggccgg ctcggcggcc tacgcggtga gcgaggcgtt cggctggaag tcgggcctgt
     3721 cgcgcggctt ccacgaggcg cgcggcttct acgtgatcat catcgcggcc acgctgatcg
     3781 gcacgctcat gagcacgctg gaggtcgacc cgatcaaggc gctggtgtgg agcgccatcg
     3841 tcaacggcgt catctcggtg cccatcatgg tggtgatgat gtggatcgga cagtcccggc
     3901 gcctcatggg ccgcttcacc atcagcctgc gccaccgcat cttcggctgg gccgccacgg
     3961 cggtgatggg cgtggccgtc gcgggcctgt tcttcacgag ctggcgcgcg ggccagtagg
     4021 tcagggcgcc atcacgaagc agttgcggcc cgcacgcttg gcctggtaga gcgcggtgtc
     4081 ggcgcgtgcc accagcgcct ccacgggctc gccctgcacg ctggtggcga cgccggccga
     4141 caccgtcacg cggaagtcgg ccggcagcgc gtcgatggcg gtccacgcat gcgcggccac
     4201 cgcgctgcgg atgcgctcga cggcgagcga cgcgctctcg accggcgtgg tgacgggcat
     4261 cagcagcacg aactcctcgc cgccccagcg cgcgaagcgg tcggtcacgc gcatgtgggc
     4321 ctgcaccagc gtgcagaagt cgcgcaggac ggcgtcgccg gcggcgtggc cgaagcggtc
     4381 gttgatcgtc ttgaagtggt cgaggtcgaa cagcgccacc gagtagggct gctgcgtgcg
     4441 gtgcgagcgc tcgcgctcgt cctgcagcag ctccatgaag tggcggcggt tgaacgcgcc
     4501 ggtcaggtcg tcatggctgg cgagctcctg gatgcgcgcc agcgacagcg tcagctgctt
     4561 gttgcgctgc gacagctcgg cgcgcagcgt gctgaactgc atgccgatca gcgacagccg
     4621 gcgcaccgcc aggaagaaga acagccacat cagcgcgatg ttgaggttcg tcagttccgg
     4681 gtaggcgaac tgcgcgcggc cgatccacag cgccagcgcg gtggtcgcgc cgaagcccag
     4741 ccatgtccag cggaactgct gcggcgtgaa gcccagcatc gcgtagttga acgacaccag
     4801 gctggaggcc aggaagatca tccacagccg cggcgccgcg acgatgaaga tcagctggat
     4861 cacgtagttc gagatcagct gcgcccacag cagccagcga tccctgaacc gcaggttcca
     4921 gccgcgcacg accgacagca ggaagaagcc cgtgctgccg acgctcagca cgaagaacca
     4981 gcccgccacc gtccacccga cggtgccgtt ccacgcgaac aggccgatca gcaaggcctg
     5041 cagcaacgaa ctgaccaccg tcatggtggt cgtcttgacg cgctgcgcct ggagctgcgg
     5101 ttgcttggag ttcattggga gcgggcgact cccggtctgc ggaaggttcc ggcgcgagca
     5161 tacgggatgc gccggcgctt gcctgtgctc ggaaacgcca cgtggcgacc gggcgacaac
     5221 gcgcaggcgg cccgcgtcag gcgcctgacg cgaccggcgg ccgcgtcagg gcatcagtgc
     5281 cgcggacggc gggtgatgtc gatcgcgccc gccctggcaa acgccagttc ggctcgcgcc
     5341 gcctggggct ggtcgtcgac gtccacccgc accaccgcgc cgccgcggcg catggcggcg
     5401 gcgtaggcgc tgacatcgtg gccgatgccc agcagcccgg cgaagaagtt gcgcaggtgg
     5461 tcgaccacgc tgtcgtcggc gaccggcgcg gccggagcgc tgtcggcggc ctcggcggtc
     5521 gacggcacgg gggtctcgat ggcgtggacg cggcgcgggt cgaagcggtc gttggcgagc
     5581 gcgacgatgg ccgcgcacgc ggccgcctgc tggtcgaacg ttccgacgac gatgtgctgc
     5641 atggtcgatt cctgtgaaag ggacgagggc gctcgcgccg cgagcgacgg cgcggcgccc
     5701 gggccggcct caggacttcg gctcggcgtc cgtcttcgtc ttgagctggc gtgccatctc
     5761 gaggtggtgc tgcagcgtgg gcagcgtctt ctgcgcccag gccttgacgt cggcgtcggt
     5821 gcccttgtcg atctccttct ggaacagctt gaccgtgtcc tcgtgcgcct tcacgccgat
     5881 cgagtcggcg tagtgctggt cgaagctgga gcccttgcgg cccgacagca tcttgatctc
     5941 ggtcttcttc aaggtcgacg gctcgtcgga caccttcacg cccttctggt cggccagcgc
     6001 cttgagttcg ctgccggcct tgccgtggtc gtcgaccatc tgctgggcga aggccttcac
     6061 gtcgccgttg gccgactgcg agacggcgac cttgctgccc tcgacctcgg cattgccggc
     6121 ctcggcggca tccttgatga aggacttgtc ctgatgagac aacttggcgg acgtgtccgc
     6181 ggcgtgggcg cccagcgcgg cggcgagcgc gacggtggcg gcggacagca gggtcaacgg
     6241 cttggattgc atgagagctc catggttggt gaacgggccc gccgcgtcgg cggcggaggg
     6301 tgcgcccggt gcggcaaggt ccatgccccg acaacagcac gggacacgtc cgcggtgcca
     6361 acatgcgtcc atgaacaccg ccgccgcccg ctcgttcacc gctcgcctgg gcctcgagct
     6421 cccgatcgtc caggccccgc tggccggctt ccagggcgtg gcgatggccg cggccgtggc
     6481 gggcgccggc gcgctcggtt cgctgcccgg cgccgcgatg acgccgcagg cgctgcgcga
     6541 cgcggtggcc gagatccgcc gcggcggccg cctgcccgtc aacctcaatt tcttctgcca
     6601 tgtcgtgccg cccgccgacc cgcagcgcga ggccgcctgg cgcgaggcgc tgcggccgta
     6661 ctacgacgag ctgggcgtgc cggagtcggc gatccagaac gggccgggcc ggctgccgtt
     6721 cggcgaggag gccgccgccg cgatcgaggc gctcaggccc gaggtcgtca gcttccactt
     6781 cggcctgccg ccggcgccgc tgctcgagcg cgtgcgggcc accggcgcgc aggtgtggtc
     6841 ctcggcgacg accgtggccg aggcgcagtg gctcgcggcg cacggcgtcg acgccgtcat
     6901 cgcgcagggc gccgacgccg gcggccatcg cgccgtcttc ctggactacg ggccggcctg
     6961 cgacctgtcg acgcagctgc gcacgttgcc gttgctcacg tccatcctcg aggtggtgcg
     7021 cgtgccggtg atcgcggcgg gcggcatctc cacggccgag ggcgtggccg cggcgctgcg
     7081 cctgggggcc agcgccgtcc agctgggcac ggccttcctc ctgtgtccgg aggcgaccac
     7141 cagcgcggtg caccgcgccg cgctgcaggg cgagggggcg cgcgacacgg cgctcaccaa
     7201 cctcttcacc ggacgtcccg cgcgcggcat cgtcaaccgg ctgatgcgcg agatgggccc
     7261 gatgagcacg ctgccgccgg ccttcccgtc ggcggtcaac gcgcttgctc cgctgcgcgc
     7321 cgcggcggag caggcggggc gcggcgactt ctcgccgctg tgggcgggcc aggacgtgag
     7381 cggctgccgc gcgatcccgg cggcgcaact ggtccgggaa ctcgcggcca agctctagcc
     7441 tgggccgtcc gggtcggtct cgaagctgaa cgcccccggc agcagcgcac ccgccgtggt
     7501 gatcaccagc gacccgtcgg ccgcgtcgct ggccaccggc gcatcggcgc cggcgaactc
     7561 gcggatgacc tggcggcagg cgccgcaggg cgtgatcggg tgcagcttcg cggccgtgac
     7621 gcccacggcc aggatacgct tggccccggc ggcgacggcc acgccgatcg ccacgcgctc
     7681 ggcgcagttg gtcagcccgt acgacgcgtt ctcgacgttg acgcccaggt gcacgcgccc
     7741 ctgctcgtcc agcaccgccg cggcgacgcg gaagtcggag tagggcgcat gcgcgttggg
     7801 cagcacggcg cgggcgcgcg ccaccagatc ggccagcgtg gcctcgtcga cgggggcggg
     7861 cgcggcgctc aggatgcgag gcccgaagtg cgcaccgcct gctcgaagga ctcggccagc
     7921 tccttcggcg ccgcgcccag cagcgcgcgg gcgtgctcca gcaggcccga cgatgcggcc
     7981 gagcggaaca cgaactgcac gatctcctcg tgatgctccg tggcggcggg atgggtcgcg
     8041 ggcaggtcaa tcgtcatgca gcggttctcc ggtgtcggcc agggccagcg attcacggat
     8101 tttctccgtc gacgcccaca gcgtgaacac gagggtcttg agcgtcgggt cgccgggagt
     8161 ctgcagcaag ggcgggatgc tgtcgagtcc gtcgacgttg agcacgccga cgatgtcgcg
     8221 cgccttcgtg a
//
LOCUS       DATCRO010000012         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_45154, whole genome shotgun sequence.
ACCESSION   DATCRO010000012 DATCRO010000000
VERSION     DATCRO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45154"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..478)
                     /locus_tag="VIN75_00295"
     CDS             complement(131..478)
                     /locus_tag="VIN75_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY8972624.1"
                     /translation="MSTDLDSLRDRLRAFALERDWGQFHSPKNLACALSVEAAELLEH
                     FQWMTDEQSRTPADAQREQIAHEAADVLLYLIQLADQLGIDLVAAADRKMALNAARYP
                     VETARGSSRKAGA"
     gene            610..3162
                     /locus_tag="VIN75_00300"
     CDS             610..3162
                     /locus_tag="VIN75_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8972625.1"
                     /translation="MPHVLARHLVPGLAITLVAGAALAAAPAQPASAPHGYDQPPKAI
                     LDVLHAPAPPTVYTSPTRQSLLLVSWQEFPPMSRVAAPFLKLAGSRIEIANHSKHDTP
                     GGYGITPCAHSFGLVAVPGGAATPVQLPAGACAGRPHWSADGKRFAFANTVKDAVELW
                     IGDAATGALHKVPGVRLNPMMDNDLQWMPDHRSLLVKLVASKGAPPPEPVVPPGPSIQ
                     ETGQGKGESSTYETRDTLKSPHDEDLFDWYAASQVALVDAATLAVTPVGPAGNVAELD
                     PAPDGQHLLVAVIHKPYSYVTTYERFPREVEVWTLGRKGAVTRHTIASIPLTDRVPVA
                     GVPTGPRDFAWRSDDPATLVWPEALDGGDWKAKVPQRDKVMLQKAPFTAPPVEIARTE
                     QRFVGFEWTEKRDVALLREYDENRHWTKTSLMDVDHPQADRRVLWDLSSDEKYADPGS
                     PVYRVQPDGQYLIRMQGDAIFLAGNGASPDGDRPFLDRLDLVTRKSERLFRSERDSLE
                     RFIAFTGPDDHAFLTWHQSTTSPPNAYLRTLAPTRDASASAPAGEAAFASNRVPVTHI
                     PDPTPAVRAISKRLVTYKRADGVDLSFTLYTPPGYVEGTRVPTILYAYPLDYADASKA
                     GQVSGSQYTFTRLRQYQLLLLAGYAIIDRASFPVIGDPKKAYDDYLPQLVADAKAAVD
                     EAVRLGVADPERIGVTGHSHGALMTVNLIAHSELFRAGVATSGSYNKTLTPFGFQNER
                     RSVWEAPEVYEKVSPFFSADKIKLPLLIMHGEDDANPGTTPLQSQKLFEAIRGNGGTA
                     RLVMLPHEPHWYSAMESQEQQIYEMVRWFDMYVKNAAPRAAAAAPAASQAAR"
BASE COUNT          496 a         1174 c         1144 g          432 t
ORIGIN      
        1 gtcctggtgc gtcgcccccg ccgcgtcccg gaagcgagca gcaaggcttc ggggtggcgg
       61 gacgcgcagg cgccccgggt cgtatcggac gcgcagcgaa cgaccgtgcg gaaaagagac
      121 agtccgcggc tcaggcgccg gccttgcggc tgctgccgcg cgcggtctcg accggatagc
      181 gcgccgcgtt gagcgccatc ttgcgatcgg cggcggcgac caggtcgatg cccagctggt
      241 cggcgagctg gatcaggtac agcagcacgt ccgcggcctc gtgcgcgatc tgctcgcgct
      301 gcgcgtcggc gggggtgcgg ctctgctcgt cggtcatcca ctggaagtgc tccagcaact
      361 cggcggcttc gaccgagagc gcgcaggcga ggttcttggg cgagtggaac tggccccagt
      421 cgcgctcgag cgcgaaggcg cgcaggcggt cgcgcaggga gtcgaggtcg gtggacatga
      481 ccggcagcat agccgccggc ggtcgcatgg tgcgccatgc catagtcggg cgctgtatcg
      541 gccgcgaccg gcgcccacga ggcgcgcggg ccacggcctc cacaccctcg atccgaagga
      601 acatcgccca tgccgcacgt cctcgcccgc cacctggtgc cgggcctcgc catcaccttg
      661 gtcgccggag ccgcgctggc ggccgccccg gcgcagccgg cctccgcccc gcacggctat
      721 gaccagccgc ccaaggccat cctcgacgtg ctgcacgcgc cggcgccgcc cacggtctac
      781 acgagcccga cgcgccagtc gctgctgctg gtctcgtggc aggagttccc gccgatgtcg
      841 cgcgtggccg cgccgttcct gaagctggcg ggcagccgca tcgagatcgc caaccacagc
      901 aagcacgaca ccccgggcgg ctacggcatc acgccgtgcg cgcactcgtt cggcctggtg
      961 gccgtgccgg gcggcgcggc cacgccggtg cagttgccgg cgggcgcctg cgcgggccgg
     1021 ccgcactggt cggcggacgg caagcgcttc gcgttcgcca acaccgtcaa ggacgccgtc
     1081 gagctgtgga tcggcgacgc cgccaccggc gcgctgcaca aggtgccggg cgtgcgcctg
     1141 aacccgatga tggacaacga tctgcaatgg atgcccgatc accggtcgct gctggtcaag
     1201 ctggtcgcca gcaagggcgc gccgccgccg gaaccggtcg ttccgcccgg ccccagcatc
     1261 caggagaccg gccagggcaa gggcgagagc agcacctacg agacgcgcga cacgctcaag
     1321 agcccgcacg acgaggacct gttcgactgg tacgcggcct cgcaggtcgc gctcgtggac
     1381 gcggcgacgc tcgcggtcac gccggtcggg ccggccggca acgtcgccga gctcgacccg
     1441 gcgccggacg gccagcacct gctggtcgcg gtgatccaca agccgtactc gtacgtcacc
     1501 acgtatgagc gcttcccgcg cgaggtcgag gtctggactc ttggcaggaa gggcgcggtg
     1561 acccgccaca cgatcgcctc gatcccgctg accgaccggg tgccggtggc cggcgtgccc
     1621 accggcccgc gcgatttcgc gtggcgctcg gacgacccgg ccacgctcgt ctggcccgag
     1681 gcgctggacg gcggcgattg gaaggccaag gtgccgcagc gcgacaaggt gatgctgcag
     1741 aaggcgccgt tcaccgcgcc gccggtggag atcgcgcgca ccgagcagcg cttcgtcggc
     1801 ttcgagtgga cggagaaacg cgacgtcgcg ctgctgcgcg agtacgacga gaaccgccac
     1861 tggacgaaga cctcgctgat ggacgtcgac cacccgcagg ctgaccggcg cgtactgtgg
     1921 gatctgtcca gcgacgagaa gtacgccgac ccgggcagcc ccgtctaccg cgtgcagccc
     1981 gacggccagt acctgatccg catgcagggc gacgcgatct tcctggccgg caacggcgcc
     2041 tcgccggacg gcgaccggcc gttcctggat cggctggacc tggtcacccg caagagcgag
     2101 cgcctgttcc gcagcgagcg cgactcgctc gagcgcttca tcgccttcac gggaccggac
     2161 gaccacgcgt tcctgacctg gcaccagtcg accaccagcc cgcccaacgc atacctgcgc
     2221 acgctcgcac ccacgcgtga cgcgagcgcg agcgcccccg cgggcgaggc agcgttcgcg
     2281 tcgaaccgcg tgcccgtcac gcacattccc gacccgacgc ccgcggtgcg tgcgatcagc
     2341 aagcgcctgg tgacctacaa gcgcgccgac ggtgtcgacc tgtcgttcac gctgtacacg
     2401 ccgccgggct acgtcgaggg cacgcgcgtg ccgacgatcc tgtacgcgta tccgctggac
     2461 tacgcggatg cgtccaaggc agggcaggtc tcgggctcgc agtacacctt cacgcgcctg
     2521 cgccagtacc agctgctgct gctggccggc tacgcgatca tcgaccgcgc gtcgttcccg
     2581 gtgatcggcg acccgaagaa ggcctacgac gactacctgc cgcaactggt cgccgacgcg
     2641 aaggcggcgg tcgacgaggc ggtgcggctg ggcgtggccg accccgagcg catcggcgtc
     2701 accggccaca gccacggcgc gctgatgacg gtcaacctga tcgcgcactc cgagctgttc
     2761 cgcgccggcg tggccacgag cggctcgtac aacaagacgc tgacgccgtt cggcttccag
     2821 aacgagcggc gctcggtgtg ggaggcgccg gaggtctacg agaaggtctc gccgttcttc
     2881 tcggccgaca agatcaagct gccgctgctg atcatgcacg gcgaggacga cgccaacccg
     2941 ggcacgacgc cgctgcagtc gcagaagctg ttcgaggcga tccgcggcaa cggcggcacc
     3001 gcgcgcctgg tcatgctgcc gcacgagccg cactggtact ccgcgatgga gtcgcaggag
     3061 cagcagatct acgagatggt gcgctggttc gacatgtacg tgaagaacgc ggcgccgcgc
     3121 gcggccgcgg cggcgccggc ggcctcgcag gcagccaggt gaacgaaggg cgtcgcgaga
     3181 cgcccatttt ttttctggtc cggccacccg tagggcgggt tagccgcagg cgtaacccgc
     3241 cgtttg
//
LOCUS       DATCRO010000013         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1068999, whole genome shotgun sequence.
ACCESSION   DATCRO010000013 DATCRO010000000
VERSION     DATCRO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068999"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VIN75_00305"
     CDS             complement(<1..716)
                     /locus_tag="VIN75_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8972626.1"
                     /translation="MSFSLEGAGVVHPNGQRALAGVTLSARDGERIAVIGPSGAGKTT
                     LLRVLGASLRPGEGEVQALGARPWQLPAAALKKLRARIGTVHQSPPIAPRLRVVTAVL
                     AGRLGEWSMLRALGSLLHPSDIAGAHEVLSRLALEDRLFERCDRLSGGQLQRVGIARV
                     LYQRPALLLADEPVSALDPTLADAAIGQLVAQSESTGATLMASLHAVDLALRWFPRVV
                     GMRAGTVMFDLPASQVTPEML"
     gene            complement(727..1584)
                     /locus_tag="VIN75_00310"
     CDS             complement(727..1584)
                     /locus_tag="VIN75_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8972627.1"
                     /translation="MKKTLTRLALALSFCAAGAGAFAQGVLRVSAIPDEAPTELQRKF
                     TPLGDYLKKETGMDVQFTPVTDYAAVVEGLATNKIDLAWLGGFTYVQARIRTNGGAVP
                     IVQRAEDEVFTSKFIVPVDSTAKTLADLKGKTFAFGAPSSTSGSLMPRYFLLQAGINP
                     EKDFKTVAFSGAHDATVAFVAASRAEAGVLNASVWDKLVEAKNPNAAKVRVLATTPTY
                     YDYNWTVRPGLDPAVTKKLTDAFLKLDPANPAHKEIMALQRASKFIPTKSSNYDGIET
                     AGKSAGLIK"
     gene            1659..2726
                     /gene="selD"
                     /locus_tag="VIN75_00315"
     CDS             1659..2726
                     /gene="selD"
                     /locus_tag="VIN75_00315"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8972628.1"
                     /translation="MNPTLMNPLAPLRLTSFSHGGGCGCKIAPGVLSQILKNQAGGLI
                     PPELMVGIETADDAAVYRLNDQQALIATTDFFMPIVDDPYEFGRIAATNAISDVYAMG
                     GRPIMALALVAMPINQLPVEVIAEIVRGGQDVCRAAGIPIAGGHTIDSVEPIYGLVAM
                     GLVHPERVRRNADAKAGDVLVLGKPLGVGVLSAALKKEQLSETGYRQLIENTTKLNTP
                     GIALSALDGVHALTDVTGFGLAGHALELARGAKLRAVIEWSRVPLLENVVVMAAEGMV
                     TGASGRNWAGYGADVKLVPGLHATTQNLLSDPQTSGGLLVSCTPDAVDAVLKVFRDEG
                     FARAAVIGRVEAGEPSLHVVP"
     gene            complement(2769..2861)
                     /locus_tag="VIN75_00320"
     tRNA            complement(2769..2861)
                     /locus_tag="VIN75_00320"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2825..2827),aa:Sec,seq:tca)
BASE COUNT          446 a          986 c         1009 g          444 t
ORIGIN      
        1 agcatctctg gcgtgacctg cgatgccggc aggtcgaaca tcaccgtccc ggcgcgcatg
       61 ccgacgacgc gcggaaacca gcgcagtgcg aggtccaccg catgcagcga tgccatcagc
      121 gtcgcgccgg tcgattcgct ctgcgcaacg agctggccga tggccgcgtc ggccagcgtg
      181 gggtcgagtg cggacaccgg ctcgtcggcc agcagcaggg cggggcgctg gtagagcacg
      241 cgtgcgatgc ccacgcgctg cagctggccg ccggagagcc ggtcgcagcg ctcgaacagg
      301 cggtcttcca gcgcaaggcg cgagagcacc tcgtgcgcac cggcgatgtc cgagggatgc
      361 agcagcgagc ccagcgcgcg cagcatcgac cattcgccga gccgccctgc aagcacggca
      421 gtgaccacgc gcagcctcgg cgcgatgggc ggcgactggt gcacggtgcc gatgcgggcg
      481 cgcagcttct tcagcgccgc cgcgggcagc tgccacggcc tggcgccgag cgcctgcacc
      541 tcgccttcgc ctggccgcag cgacgcgccg agcacgcgca gcagcgtggt cttgcctgcc
      601 cccgaagggc cgatgacggc gatgcgttcg ccatcgcgcg cggacagcgt cacgccggcc
      661 agcgcgcgct ggccgttggg atggacgacg cccgcgcctt ccagcgaaaa actcatccgc
      721 gcaggctcac ttgatcaggc cagccgactt gcccgccgtc tcgatcccgt cgtagttcga
      781 cgacttggtc gggatgaact tgctcgcgcg ctgcagcgcc atgatttcct tgtgcgccgg
      841 gttggccggg tcgagcttga ggaaggcgtc ggtcagcttc ttcgtgaccg cgggatcgag
      901 gccggggcgc acggtccagt tgtagtcgta gtaggtcggc gtggtggcca gcacgcgcac
      961 cttggcggcg ttggggttct ttgcctccac cagcttgtcc cacactgacg cgttgagcac
     1021 gccggcttcg gcgcgcgagg cggccacgaa ggccacggtg gcgtcgtgcg cgcccgagaa
     1081 cgcgacggtc ttgaagtcct tctcggggtt gatgcccgcc tgcagaagga agtagcgcgg
     1141 catcaggctg cccgaggtgg aggagggcgc gccgaacgcg aaggtcttgc ccttcaggtc
     1201 ggccagcgtc ttggcggtgc tgtccaccgg cacgatgaac ttgctggtga acacctcgtc
     1261 ctccgcgcgc tgcacgatgg gcaccgcgcc gccgttggtg cggatgcgcg cctgcacgta
     1321 ggtgaagccg ccgagccagg ccaggtcgat cttgttggtg gccaggccct cgaccacggc
     1381 ggcgtagtcg gtcacgggcg tgaactgcac gtccatgccg gtttccttct tgagatagtc
     1441 gcccagcggc gtgaacttgc gctgaagctc ggtgggcgcc tcgtcgggaa tggccgagac
     1501 gcgcagcacg ccctgcgcga aggcgccggc gcctgcggcg cagaagctca gggcaagggc
     1561 cagtcgggtg agtgtctttt tcatggatgc gcctcggagg cttgcggttt tggaaaggac
     1621 gggattgtag aaagcgggcc cgattcgata atcgcgccat gaacccgact ctcatgaacc
     1681 cgctcgcgcc cctgcgcctc acgagctttt cgcatggtgg cggctgcggc tgcaagatcg
     1741 cgccaggcgt gctgtcgcag atcctgaaga accaggccgg cgggctgatc ccgcccgagc
     1801 tcatggtggg catcgagacc gccgacgacg cagccgtcta ccggctcaac gaccagcagg
     1861 cgctgatcgc gaccaccgac ttcttcatgc ccatcgtcga cgacccgtac gagttcggcc
     1921 gcatcgccgc gaccaacgcg atcagcgacg tgtacgcgat gggcgggcgc ccgatcatgg
     1981 cgctggcgct cgtcgccatg cccatcaacc agctgcccgt ggaggtcatc gccgagatcg
     2041 tgcgcggcgg acaggacgtg tgccgcgcgg ccggcatccc cattgcgggc ggccatacca
     2101 tcgattcggt cgagcccatc tacggcctcg tcgccatggg cctggtgcac cccgagcgcg
     2161 tacgccgcaa cgccgacgcg aaggccggcg acgtgctcgt gctgggcaag ccgctgggcg
     2221 tgggcgtgct ctccgccgcg ctcaagaagg aacagctctc cgagaccggc tatcgtcagc
     2281 tcatcgagaa cacgacgaag ctcaacaccc ccggcatcgc gctgtcggct ctcgacggcg
     2341 tgcatgcgct cacggacgtc accggcttcg gcctggccgg ccacgcgctc gagctggcgc
     2401 gcggcgcgaa gctcagggcc gtcatcgagt ggtcgagggt gccgctgctc gagaacgtgg
     2461 tggtgatggc cgccgaaggc atggtcaccg gcgcatccgg ccgcaactgg gccggatacg
     2521 gcgccgacgt gaaactcgtc cccggcctgc acgccaccac gcagaacctg ctgagcgatc
     2581 cgcagacctc gggcggcctg ctcgtgagct gcacgcccga tgcggtcgat gcggtgctga
     2641 aggtcttccg cgacgagggc ttcgcgcgcg ccgcagtcat cggccgcgtg gaagccggcg
     2701 agccgtcgct gcacgtggtg ccctgaagaa gcagttctac gccgactcgt cgaatgcccg
     2761 ctcgacggcg gagggaggaa ggagtcgaac cttcccggcg acggctggcg ccaccaaacg
     2821 ggtttgaagc ccggccgccc caccaggggc gatctccctc catgatccga ttcgtcgttc
     2881 aaggc
//
LOCUS       DATCRO010000014         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_382705, whole genome shotgun sequence.
ACCESSION   DATCRO010000014 DATCRO010000000
VERSION     DATCRO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382705"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIN75_00325"
     CDS             complement(<1..554)
                     /locus_tag="VIN75_00325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8972629.1"
                     /translation="MQAQNLASGSPADHSILLDNWRGWAIIFVVAGHFWHVPAIDSGR
                     VGVELFFVLSGRLMADLLFVKHTPLPTFFQRRFTRVLPLSLMFVFTAWIAFPPGPLHL
                     PDKAALASLGMVVNYTQLVGISAPVTDHYWSLCVEEHSYVALALLALALRRSGRDRGA
                     TAGWICLALAALMMLNGVRLWLLT"
     gene            834..1637
                     /locus_tag="VIN75_00330"
     CDS             834..1637
                     /locus_tag="VIN75_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8972630.1"
                     /translation="MQGKALATIAAAIVSLAALPAAASTATASLTDFHVQLIDLNPND
                     GIAPSLTFQNLQGGSFIAAESGVPGNVLTDTHQGGAPLGAGSSSSTRGAASALAQFMG
                     DPLGQGASALASAALGARGQYGASTVQLGDGAIYAPFTLSADTRLVITADASASALST
                     FADPQTDTWASVFLKLTDASGTGSVSTDTVSAEQFAMIGGTPTATVDDHVMTITFENL
                     TGGDLGGIFFGSIDAYAADLVPAVPEPGTLPLTFAGLGLLAWRLRRRAR"
     gene            complement(1764..3308)
                     /gene="amt"
                     /locus_tag="VIN75_00335"
     CDS             complement(1764..3308)
                     /gene="amt"
                     /locus_tag="VIN75_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8972631.1"
                     /translation="MRKLLASLALGLAVLGFSATPSLAADAAASAPAASAPAAAASAP
                     AADASAPAAAAAPASAAASTPTIVETVNKGDVSWMLVSTALVIMMSIPALALFYGGLV
                     RSKNMLSVLMQVFVTFSLIVVLWAIYGYSFTFTQGSGWLSPFFGGASRVFLSGTFDAA
                     KGTFSNAATFSKGVVLPELVYVVFQATFAAITCCLIVGSFAERMKFSAVLAFMVLWFT
                     FSYVPIAHMVWFWQGPDAYTGKDVVDAQNATAGLIWQWGALDFAGGTVVHINAAVAGL
                     VGAFMVGKRIGYGKEAFTPHSTTLTMVGASLLWVGWFGFNAGSALEANGSAALAFVNT
                     FTATAAAVLSWSLGEGLMKGKASMLGAASGAVAGLVAITPAAGNVGLMGAIVLGLIAG
                     FAGLWGVHGLKKLLGADDSLDVFGVHGVCGIVGALLTGILNNQALGGPGMVTDWVTGA
                     VGSNGMWAQFLIQAKGVGVTIVWSGVVSIIAYKLVDLTIGLRVTEDEEREGLDITSHG
                     ETAYAK"
     gene            complement(3375..3713)
                     /gene="glnK"
                     /locus_tag="VIN75_00340"
     CDS             complement(3375..3713)
                     /gene="glnK"
                     /locus_tag="VIN75_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8972632.1"
                     /translation="MKMVTAVVKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVDDALVDQVIEAIERSARTGKIGDGKIFVSPLEQVVRI
                     RTGETGKDAL"
     gene            complement(3799..>4290)
                     /locus_tag="VIN75_00345"
     CDS             complement(3799..>4290)
                     /locus_tag="VIN75_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY8972633.1"
                     /translation="DLYGGSKGDIIKDTLTYDVGVLAYVYLGQKFHEIGMANPDTVEV
                     YGALTYGVFTGKVSISTTPLFGVPKSTGSQYYDLSANFDLGAGFTLTPDVAYQSITGD
                     VSKAAGLSYGAYSLTLGKDFGKGLSASLQVIATDAKKEDYQTSNGKFTGRTGVVAGVK
                     YSF"
BASE COUNT          701 a         1460 c         1515 g          614 t
ORIGIN      
        1 tgcgtcagca gccacagccg cacgccgttg agcatcatca gggcggccag cgccaggcag
       61 atccagccgg ccgtcgcccc gcggtcgcgg ccgctgcggc gcagggcgag cgccagcagc
      121 gcgagcgcga cgtagctgtg ctcctcgacg cacaacgacc agtagtggtc ggtcaccggc
      181 gcgctgatgc cgaccagttg cgtgtagttg accaccatgc ccagcgacgc cagcgcggcc
      241 ttgtcgggca ggtgcagcgg gccgggcggg aacgcgatcc aggccgtgaa cacgaacatc
      301 agcgacagcg gcagcacgcg ggtgaaccgg cgctggaaga aggtgggcag cggcgtgtgc
      361 ttgacgaaca gcaggtcggc catcaggcgg cccgacagca cgaagaacag ctcgacgccg
      421 acgcgccccg agtcgatcgc gggcacgtgc cagaaatggc cggcgacgac gaagatgatc
      481 gcccagccgc gccagttgtc gagcaggatg gaatgatcgg cgggcgagcc ggaggcgagg
      541 ttctgggcct gcacgatgtg gtccttcctt gatgcgccgc gcgcgcaagg cgcgatgccc
      601 cagggggtac cgcgacgagg ggatgccagg gccttgcgct cgacagggcg gtgcccgaca
      661 gttcgagggc cgggcgggca atccgaatgc cgaaatggcg ccagcaagcg atttgctgac
      721 gacaaataca cccggttgcg acgtgcccga gcagggggct gggcggcgtc ctagactggt
      781 gcatgcgcgc ggccgcagag ggcggcacgc gaggagacaa cagggaaccc aagatgcaag
      841 gaaaagcgct cgcgaccatc gccgcggcca tcgtgtcgct ggctgcgctg ccggcggcgg
      901 ccagcacggc cacggcgtcg ctgacggact tccacgtcca gctgatcgac ctgaatccca
      961 atgacggcat cgcgccgtcg ctcaccttcc agaacctgca gggcggttcg ttcatcgcgg
     1021 ccgagagcgg cgtgccgggc aacgtcctca ccgacaccca ccagggcggc gcgccgctgg
     1081 gcgccggttc gtcgtcgagc acgcgcggcg ccgcctcggc actggcgcag ttcatgggcg
     1141 acccgctcgg gcagggggcc agcgccctcg cgtcggctgc gctcggcgcc cgtggccagt
     1201 acggcgccag caccgtgcag ctgggcgacg gggccatcta cgcgccgttc acgctgtcgg
     1261 ccgacacgcg gctggtgatc acggccgacg cgagcgcgtc cgcgctgtcg acctttgccg
     1321 acccgcagac cgacacctgg gcctcggtgt tcctgaagct gaccgacgcc agcggcacgg
     1381 gcagcgtgag caccgacacg gtcagcgccg agcagttcgc gatgatcggc ggcacgccca
     1441 ctgcgacggt cgacgaccac gtcatgacga tcacgttcga gaacctgacc ggcggcgacc
     1501 tcggcggcat cttcttcggc tcgatcgacg cctacgcggc cgacctcgtg ccggcggtgc
     1561 ccgagcccgg cacgctgccg ctgacgttcg ccggcctggg cctgctcgcc tggcgcctgc
     1621 gccgccgcgc gcgctgacgc gtcccgcgcc atgacgaagg ccccgcgagt cggggccttt
     1681 tcgttcctgc catgaaaaga gggctgccct ggcggacagc ccttcgcatt cgcgagcccg
     1741 cgcggcgggc tcgttgaagc ggcttacttg gcgtaggccg tctcgccgtg ggacgtgatg
     1801 tccaggcctt cgcgctcttc gtcttccgtc acgcgcaggc cgatggtcag gtcgaccagc
     1861 ttgtaggcga tgatggagac gacgcccgac cagacgatcg tcacgccgac gcccttggcc
     1921 tggatcagga actgcgccca catgccgttg gagccgaccg cgccggtgac ccagtcggtg
     1981 accatgcccg gcccgccgag cgcctggttg ttcaggatgc cggtcagcag cgcgccgacg
     2041 atgccgcaga caccgtgcac gccgaacacg tccagggagt cgtccgcgcc cagcagcttc
     2101 ttgaggccat gcacgcccca caggcccgcg aagccggcga tcaggccgag gacgatcgcg
     2161 cccatcaggc cgacgttgcc ggcggccggg gtgatcgcca cgaggccggc gaccgcgccc
     2221 gaggcggcgc ccagcatcga ggccttgccc ttcatcaggc cttcgcccag cgaccacgac
     2281 agcacggcgg cggcggtggc ggtgaacgtg ttgacgaagg ccagcgcagc cgagccgttg
     2341 gcttccagcg cggagcccgc gttgaagccg aaccagccca cccacagcag cgaggcgccc
     2401 accatggtca gcgtggtcga gtgcggcgtg aacgcttcct tgccgtagcc gatgcgcttg
     2461 cccaccatga acgcgccgac caggccggcg acggcggcgt tgatgtgcac cacggtgccg
     2521 ccggcgaagt ccagcgcgcc ccactgccag atcaggccgg cggtggcatt ctgggcgtcg
     2581 accacgtcct tgccggtgta ggcgtccggg ccttgccaga accagaccat gtgcgcgatg
     2641 ggcacgtagc tgaacgtgaa ccacagcacc atgaaggcca gcacggcgga gaacttcatg
     2701 cgttccgcga acgagccgac gatcaggcag cacgtgatgg ccgcgaacgt cgcctggaac
     2761 acgacgtaga ccagctccgg cagcacgacc cccttgctga acgtggctgc gttggagaac
     2821 gtgcccttgg ccgcgtcgaa ggtgccgctc aggaacacgc gcgaggcgcc gccgaagaac
     2881 ggcgacagcc acccgctgcc ctgcgtgaac gtgaagctgt agccatagat ggcccacagc
     2941 accacgatca gcgagaacgt gacgaacacc tgcatcagca cggacagcat gttcttgctg
     3001 cgcaccaggc caccgtagaa cagcgccagc gcggggatcg acatcatgat gacgagcgcg
     3061 gtggacacga gcatccagga cacgtcgccc ttgttgaccg tctcgacgat cgtcggcgtc
     3121 gaagcggcgg cggacgcggg ggcggctgcg gcagccgggg cggaggcgtc ggcggcgggc
     3181 gcgcttgcgg cggcggccgg ggcggacgcg gcgggcgccg aggccgcggc gtcggccgcg
     3241 agcgacggcg tcgcggagaa cccgaggacc gcgaggccca gggcgaggga agcgagaagc
     3301 tttctcatgg tggcagagct ttcaggagtt ggaagcgtgt ggtgtcgaaa acgtgcggga
     3361 agtcgtgcgg cgcctcagag ggcgtccttg ccggtctcgc cggtgcggat gcgcaccacc
     3421 tgctcgagcg gcgagacgaa gatctttccg tcgccgatct ttccggtgcg cgcggagcgc
     3481 tcgatggcct cgatgacctg gtcgacgagg gcatcgtcga cggcggcttc gatcttcacc
     3541 ttgggcagga agtccacgac gtactcggcg ccgcggtaca gctccgtgtg gcccttctgg
     3601 cggccgaagc ccttcacttc ggtcaccgtg atgccctgca cgccgatggc gctcagggcc
     3661 tcacgcactt cgtcgagctt gaagggcttc acgacggcag tcaccatctt catggtctta
     3721 tctccaggct ggcggacgcc ctcgcgtccg gttcttgtga aacggggtgc ggcccggttc
     3781 gcgccgggcc gcgggacgtc agaacgagta cttcacgccg gcgacgacgc cggtgcggcc
     3841 ggtgaacttg ccgttggagg tctggtagtc ctccttcttc gcgtcggtgg cgatcacctg
     3901 cagcgaggcg gacaggccct tgccgaagtc cttgcccagc gtcagcgagt acgcgccgta
     3961 cgacaggccg gcggccttgg acacgtcgcc cgtgatgctc tggtaggcga cgtccggcgt
     4021 cagcgtgaag cccgcgccga ggtcgaagtt ggcgctgagg tcgtagtact ggctgcccgt
     4081 gctcttgggc acgccgaaca gcggcgtcgt gctgatcgag accttgccgg tgaacacgcc
     4141 gtaggtcagc gcgccgtaga cctcgaccgt gtccgggttg gccatgccga tctcgtggaa
     4201 cttctggccc aggtacacat aggccagcac gccgacgtcg tacgtcaacg tgtccttgat
     4261 gatgtcgccc ttggagccgc cgtacaggtc
//
LOCUS       DATCRO010000015         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_787778, whole genome shotgun sequence.
ACCESSION   DATCRO010000015 DATCRO010000000
VERSION     DATCRO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787778"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VIN75_00350"
     CDS             <1..622
                     /locus_tag="VIN75_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8972634.1"
                     /translation="AQKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRK
                     LAERSQVAAEEIANVASSSVELAEKAGQLLDTIVPNIRKTSDLVQEITAASEEQSAGV
                     GQINSAVTQLSQTTQQNASSSEELAATAEEMSSQAEQLQQTMSFFKLSGATHVRAASA
                     TIASARRSSARARPSVARAAAPSAGRMAGVVAADADGVDESHFARF"
     gene            633..1148
                     /locus_tag="VIN75_00355"
     CDS             633..1148
                     /locus_tag="VIN75_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8972635.1"
                     /translation="MNAKVSIAAGTEPAQYLTFMLAQEQFAVGILGIKEIIEYQGVTE
                     VPMMPACVRGVINLRGAVVPVVDLLARFGRASSAVTKRTCVVIVEAACGTDERHVMGL
                     LVDAVNEVLDIAPADIEPPPAFGARLRSDFIQGVGKVRGKFVLLLDVDRVLSVDEIAG
                     YAAPVDEPVAA"
     gene            1196..>2169
                     /locus_tag="VIN75_00360"
     CDS             1196..>2169
                     /locus_tag="VIN75_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEY8972636.1"
                     /translation="MFGNLKVGTRIALGFGAVVVLMLVISITAVWRLGTLNQATTSIT
                     EDIYPEVAYTADVVRVALEDGRHLREMLLARSDADIEQAWDAAAAARARGDELMKKLD
                     HEIDTDKGRELFKAAVDRQAAIEARYARLHELARSDREKGAEYLKNDFSPANQAYRAA
                     LDELQKYEVSRMEAASRNSAEVYASTRLVVIGLAAVAMLMAVAVALLITRDLLKVLGG
                     EPSYAAEVMRKVAVGDFTVDVSVRKGDTSSLLHTTREMVRSAGSSVDDVVRVMGAMAG
                     GDLTQTITAQYAGSFGKMKDYVNETVEKLSQVVTEVNGGAEALAGASE"
BASE COUNT          352 a          738 c          769 g          310 t
ORIGIN      
        1 cgcgcagaag atcggcatca tcgacgacat cgcctaccag acgaacctgc tggcgctgaa
       61 cgcggccatc gaggcggcgc gggcgggcga gcacggcaag ggcttcgcgg tcgtcgcggc
      121 cgaggtgcgc aagctggccg agcgcagcca ggtcgccgcc gaggagatcg ccaacgtcgc
      181 cagctccagc gtggagctcg ccgagaaggc cggccagctg ctggacacga tcgttcccaa
      241 catccgcaag acgtcggatc tggtgcagga gatcaccgcc gcgtcggagg agcagtcggc
      301 gggcgtcggc cagatcaatt ccgccgtcac gcagctgagc cagacgacgc agcagaacgc
      361 gtcgagttcg gaggagctcg ccgcgacggc cgaggagatg agttcgcagg ccgagcagct
      421 gcagcagacc atgtcgttct tcaagctgtc gggcgcgacg catgtgcgcg ccgcgagcgc
      481 gacgatcgcg tcggcccgac gctcgtccgc gcgtgcgcgc ccgtccgtgg cgcgcgcggc
      541 cgccccgtcc gcgggccgca tggccggcgt ggtcgcggcc gacgccgacg gcgtcgatga
      601 atcccatttc gcccgcttct gaggacacgg ccatgaatgc caaggtttcc atcgccgcgg
      661 gcaccgagcc cgcgcaatac ctcaccttca tgctggcgca ggagcagttc gccgtcggca
      721 tcctgggcat caaggagatc atcgagtacc agggcgtcac cgaggtgccg atgatgccgg
      781 cctgcgtgcg cggcgtgatc aacctgcgcg gcgccgtcgt tccggtggtc gacctgctgg
      841 cgcgcttcgg ccgcgcctcg tccgcggtca ccaagcgcac ctgcgtcgtc atcgtcgagg
      901 ccgcgtgcgg caccgacgaa cgccacgtga tgggcctgct ggtcgacgcg gtcaacgagg
      961 tgctggacat cgcgccggcc gacatcgagc cgccgcccgc gttcggtgcg cgcctgcgca
     1021 gcgatttcat ccagggcgtc ggcaaggtgc gcggcaagtt cgtgctgctg ctcgacgtcg
     1081 atcgcgtgct gtccgtcgac gagatcgccg gatacgccgc gcctgtcgac gagcccgtgg
     1141 cggcctgacc gggcgaccgc acgttttcca tcttctgaaa gccaaggaat ctcccatgtt
     1201 cggcaacctc aaggtcggta cgcgcatcgc gctgggtttc ggcgccgtcg tcgtgctgat
     1261 gctcgtgatc tccatcaccg cggtatggcg gctgggcacg ctcaaccagg ccaccacctc
     1321 gatcaccgag gacatctacc ctgaagtcgc gtacaccgcg gacgtcgtgc gcgtggcgct
     1381 cgaggacggg cgccacctgc gcgagatgct gctggcgcgc tccgacgccg acatcgagca
     1441 ggcctgggat gcggccgccg ccgcccgcgc ccgcggcgac gagctgatga agaagctcga
     1501 ccacgagatc gacaccgaca agggccgcga gctgttcaag gccgccgtcg accgccaggc
     1561 ggcgatcgag gccaggtacg cccgcctgca cgagctcgcg cgcagcgacc gcgagaaggg
     1621 cgcggaatac ctgaagaacg acttttcgcc ggccaaccag gcctatcgcg ccgcgctgga
     1681 cgaactgcag aagtacgagg tgtcgcgcat ggaggccgcc tcgcgcaaca gcgccgaggt
     1741 ctacgcctcg acgcgcctgg tcgtgatcgg cctggccgcg gtcgcgatgc tgatggcggt
     1801 ggccgtggcg ctcttgatca cgcgcgacct gctcaaggtg ctgggcggcg agccgtcgta
     1861 cgcggccgag gtgatgcgca aggtcgctgt cggcgacttc acggtggacg tgtcggtgcg
     1921 caagggcgat acgagcagcc tgctgcacac gacccgcgag atggtgcgct cggccggcag
     1981 cagcgtcgac gacgtcgtgc gcgtgatggg cgcgatggcc ggcggcgacc tgacgcagac
     2041 catcaccgca cagtacgccg gctcgttcgg caagatgaag gactacgtca atgagacggt
     2101 cgagaagctg tcgcaggtcg tgaccgaggt caacggcggc gccgaggcgc tggccggcgc
     2161 gtccgagga
//
LOCUS       DATCRO010000016         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_720260, whole genome shotgun sequence.
ACCESSION   DATCRO010000016 DATCRO010000000
VERSION     DATCRO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720260"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="VIN75_00365"
     CDS             <1..2001
                     /locus_tag="VIN75_00365"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEY8972637.1"
                     /translation="PPLRSAAGRVRLPGSKSISNRVLLLAGLAAGTTAVHDLLDSDDT
                     RVMMAALEALGCQLRREGAVLHVTGIGGQLTTRQAKLFLGNAGTAMRPLSAALAVLAT
                     LQGGEFDLTGVPRMHERPIGDLVDALRQLGCPVQYLGHAGYPPLRVGNDRPGPLSTHE
                     PVRVRGDVSSQFLTALLLALPLATGERAITIAVEGELISKPYIDITLNLLARFGIEVQ
                     RDPANPYAAFTLPQGSQYRSPGAIHVEADASSASYFVALGALAGIEAPVRIEGVGLDS
                     IQGDIRFVEAARAMGAQVEGGAGWLEVRRGAWPLKAITLDCNHIPDAAMTLAVMALYA
                     DGSTRLTNIASWRVKETDRIAAMAAELRKMGGSVVEGPDFIEVTPPASPSHWLAGSIR
                     TYDDHRMAMCLSLAAFNALAGARPPRPVRILDPQCVAKTFPDYFETLFGLARADVADI
                     PVITIDGPTASGKGTLASEVARRLGYELLDSGALYRATALAVQQAGADPSDAAAVAAL
                     AARLDLRFEADRAFLDGRDVTDALRLESSGVMASQISVHPAVRQALHGLQLAFRRLPG
                     LVADGRDMGTVVFPAAPLKVFLTASVEQRAARRHNQLISRGIPANIDSLLADLRARDE
                     RDTNRAVAPLKPAPDAVLLDNSGMSIDEGVDAVLEAWNKRHV"
     gene            2217..3935
                     /gene="rpsA"
                     /locus_tag="VIN75_00370"
     CDS             2217..3935
                     /gene="rpsA"
                     /locus_tag="VIN75_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8972638.1"
                     /translation="MPKTQAITDFAPGSFAAMFEESLKSSEMRSGEVITAEVVRIDHS
                     FVVVNAGLKSEAYVPIDEFKNDQGEVEVQVGDFVSVAIDAVENGYGDTILSRDKAKRL
                     ASWLSLETALESGDFVTGTVSGKVKGGLTVLVNGIRAFLPGSLLDTRPVKDMSPFEGK
                     TMEFKVIKLDRKRNNVVLSRRAVVEASMGEERAKLLETLSEGAIVNGVVKNITEYGAF
                     VDLGGIDGLLHITDMAWRRVRHPSEVVTVGQELTAKVLKFDAEKNRVSLGLKQLGDDP
                     WMGVARRYPTGTRLFGKVTNIADYGAFVEIEPGIEGLVHVSEMDWTNKNVAPSKTVTL
                     GEEVEVMVLEIDEDKRRISLGMKQCRANPWEEFAANVHRGDKVHGPIKSITDFGVFVG
                     LAQGIDGLVHLSDLSWHEPGEQAVRNYKKGQEVEAVVLGIDVERERISLGIKQLDGDP
                     FANYTSVNDRGALVTGKVKSVDPRGAEIQLTEDVTGYLRASELTRDRVEDARTLLKEG
                     DEVNAMIINVDRKTRSIQLSIKAKDNADQQEAMQRISQSNERENAGTTSLGALLRAKL
                     DGGSKE"
     gene            3942..4244
                     /locus_tag="VIN75_00375"
     CDS             3942..4244
                     /locus_tag="VIN75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY8972639.1"
                     /translation="MTRSDLVAQLAERFTQITVRDTEFAVKTVLDAMSDALARGHRIE
                     IRGFGSFSISRRPPRVGRNPRSGEQVTIPEKLVPHFKPGKALREAVDKELPAGNKS"
     gene            complement(4450..5016)
                     /locus_tag="VIN75_00380"
     CDS             complement(4450..5016)
                     /locus_tag="VIN75_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8972640.1"
                     /translation="MSPAPVELLFIQGASAGAHEADRVLSDALSRALGPAFRVLFPHM
                     PHEDAPDNDVWKRRISTTLRQTRATFLVAHSAGAALVADLLAQGPPDELAALQGVILL
                     APPFVGRGGWQLDGFHLDAAVDPERLRGLTLHLYFGLADTTVPPSHADLYGVLFPDAT
                     IRRLPGCGHQFEGFMAEVARDVRALAQA"
     gene            5141..5566
                     /locus_tag="VIN75_00385"
     CDS             5141..5566
                     /locus_tag="VIN75_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8972641.1"
                     /translation="MWTHSTSIATTATPQALWRLFEDVAGWTNWNAGIERIALNGPFA
                     AGSTFDMKAPGMDTFRSTLLDVRPNASFVDETVLEGTRFLVSHELQRLPSGGTRVTYA
                     ITVEGPEAATLGPLVTADFPEVLAALKALAERAPAGATQ"
BASE COUNT          901 a         2039 c         1862 g          788 t
ORIGIN      
        1 ccgcccctgc gatcggccgc cggccgcgtg cgcctgcccg gctccaagag catctccaac
       61 cgcgtgctgc tgctggccgg cctggccgcc ggcaccacgg cggtgcacga cctgctcgac
      121 tccgacgaca cccgcgtgat gatggccgcg ctcgaggcgc tcggctgcca gctgcgacgc
      181 gaaggcgcgg tgctgcacgt caccggcatc ggcggccagc tcaccaccag gcaggccaag
      241 ctgttcctgg gcaacgccgg cacggcgatg cgcccgctca gcgccgcgct ggccgtgctc
      301 gccacgctgc agggcggcga gttcgacctc acgggcgttc cgcgcatgca cgagcgcccc
      361 atcggcgacc tcgtcgacgc gctgcgccag ctcggctgcc cggtgcagta cctcggccac
      421 gccggctacc cgccgctgcg cgtgggcaac gaccgcccgg gcccgctgtc cacgcacgag
      481 ccggtgcgcg tgcgcggcga cgtctccagc cagttcctca ccgccctgct gctggcgctg
      541 ccgctggcca ccggcgagcg cgcgatcacg atcgccgtcg agggcgagct gatctccaag
      601 ccctacatcg acatcacgct gaacctgctc gcgcgcttcg gcatcgaggt gcagcgcgac
      661 ccggcgaacc cctacgccgc gttcacgctg ccgcagggct cgcagtaccg gtcgccgggc
      721 gccatccacg tcgaggcgga cgcctcgtcg gcgtcgtact tcgtggcgct gggcgcgctc
      781 gccggcatcg aggcgccggt gcgcatcgag ggcgtgggcc tcgattcgat ccagggcgac
      841 atccgcttcg tcgaggcggc gcgcgcgatg ggcgcccagg tcgaaggcgg cgccggctgg
      901 ctcgaggtgc gccgcggcgc ctggccgctc aaggccatca cgctcgactg caaccacatc
      961 cccgacgccg cgatgacgct ggccgtgatg gcgctgtacg ccgacggcag cacgcgcctg
     1021 accaacatcg ccagctggcg cgtcaaggag accgaccgca tcgccgcgat ggccgccgag
     1081 ctgcgcaaga tgggcggcag cgtcgtcgag gggcccgact tcatcgaggt cacgccgccc
     1141 gcgtcgccgt cgcactggct ggccggctcg atccgcacct acgacgatca ccggatggcg
     1201 atgtgcctgt cgctggccgc gttcaacgcg ctggccggcg cgcggccgcc gcgcccggtg
     1261 cgcatcctcg acccgcagtg cgtcgccaag accttccccg actacttcga gacgctgttc
     1321 ggcctggccc gcgccgacgt cgccgacatc ccggtgatca cgatcgacgg ccccaccgcc
     1381 tcgggcaagg gcacgctggc gtccgaggtg gcgcgccgcc tcggctacga gctgctcgac
     1441 tcgggcgcgc tgtaccgcgc cacggcgctg gccgtgcagc aggccggcgc cgacccgtcg
     1501 gacgccgccg ccgtcgccgc gctcgccgcg cggctggacc tgcgcttcga ggccgaccgc
     1561 gccttcctcg acggccgcga cgtcaccgac gccctgcgcc tggaatccag cggcgtgatg
     1621 gcctcgcaga tctccgtgca cccggcggtg cgccaggcgc tgcacggcct gcagctggca
     1681 ttccgccggc tgccgggcct ggtggccgac ggccgcgaca tgggcaccgt ggtgttcccg
     1741 gccgccccgc tgaaggtctt cctgaccgcc agcgtcgagc aacgcgcggc ccgccggcat
     1801 aaccaattga tttcccgggg tattcctgct aatatcgaca gtctcctcgc cgacctccgg
     1861 gcgcgggacg agcgcgacac gaatcgcgcc gtcgcgcccc tgaagccggc gccggacgcg
     1921 gtgctgctcg acaactccgg catgtccatc gacgaaggtg tcgatgccgt gctcgaagcc
     1981 tggaacaagc gccacgtcta gcgtcaagga gatggccgcc gcgaggtggc caagcaggcc
     2041 cgccgcctgt gtcccgcgcg ccctcgcagg cgccgccgga tgcgtcgaac acggactccc
     2101 cgtccggttc ggggcggtgg tcgttccacc ctaaccctgc accctccccg ggttgcaaac
     2161 cgccggcagc gctcgttccc tatcgcggtc gcaccacgcc ggtcacagga aatttcatgc
     2221 ccaagaccca agccatcact gatttcgccc ccggttcctt cgcggcgatg ttcgaggaat
     2281 cgctcaagag cagcgaaatg cgctccggcg aagtcatcac tgccgaagtg gtgcgcatcg
     2341 atcacagctt cgtcgtcgtc aacgccggcc tcaagtccga agcctacgtg cccatcgacg
     2401 aattcaagaa cgatcaaggc gaagtcgaag tccaggtcgg tgacttcgtt tccgtcgcga
     2461 tcgacgccgt cgagaacggc tacggcgaca ccatcctgtc gcgcgacaag gccaagcgcc
     2521 tcgcctcgtg gctgtcgctc gagaccgccc tggaatccgg cgacttcgtc accggcaccg
     2581 tcagcggcaa ggtcaagggc ggcctgaccg tcctggtcaa cggcatccgc gccttcctgc
     2641 cgggctccct gctggacacg cgtccggtca aggacatgag cccgttcgag ggcaagacca
     2701 tggaattcaa ggtcatcaag ctcgaccgca agcgcaacaa cgtcgtgctg tcgcgccgtg
     2761 ccgtggtcga ggcctcgatg ggtgaagagc gcgccaagct gctcgagacc ctgtccgaag
     2821 gcgccatcgt caacggcgtg gtcaagaaca tcaccgaata cggtgcgttc gtcgacctcg
     2881 gcggcatcga cggcctgctg cacatcaccg acatggcctg gcgccgcgtc cgtcacccga
     2941 gcgaagtggt gacggtcggc caggaactga cggccaaggt cctgaagttc gacgccgaga
     3001 agaaccgtgt ctcgctgggc ctgaagcagc tgggcgacga tccgtggatg ggcgtggccc
     3061 gccgctaccc gacgggcacg cgcctgttcg gcaaggtcac gaacatcgcc gactacggcg
     3121 cgttcgtcga gatcgagccg ggcatcgaag gcctggtgca cgtctcggaa atggactgga
     3181 cgaacaagaa cgtcgccccg tccaagaccg tcacgctggg cgaggaagtc gaagtcatgg
     3241 tcctcgagat cgacgaagac aagcgccgca tctcgctggg catgaagcag tgccgcgcca
     3301 acccgtggga agagttcgcc gccaacgtgc atcgtggcga caaggtccac ggcccgatca
     3361 agtcgatcac cgacttcggc gtgttcgtgg gcctggccca gggcatcgac ggcctggtgc
     3421 acctgtccga cctgtcctgg cacgagccgg gcgagcaggc cgtgcgcaac tacaagaagg
     3481 gccaggaagt cgaggccgtg gtcctgggca tcgacgtcga gcgcgagcgc atctcgctgg
     3541 gcatcaagca gctggacggc gacccgttcg ccaactacac ctcggtcaac gaccgtggcg
     3601 cgctggtgac cggcaaggtc aagtcggtcg acccgcgcgg cgccgagatc cagctgacgg
     3661 aagacgtcac cggctacctg cgtgcctcgg agctgacgcg cgaccgcgtg gaagacgccc
     3721 gcaccctcct caaggaaggc gacgaagtca acgccatgat catcaacgtc gaccgcaaga
     3781 cgcgttcgat ccagctgtcg atcaaggcca aggacaacgc cgaccagcag gaagccatgc
     3841 agcgcatctc gcagtcgaac gagcgcgaga acgccggcac gacgagcctg ggcgccctgc
     3901 tgcgcgccaa gctcgacggc ggttccaagg aataaggcgc gatgacgcgg tccgacctcg
     3961 tcgctcagct cgctgagcgg ttcacgcaga tcaccgtgcg tgacaccgag ttcgcggtca
     4021 agaccgtgct cgatgcgatg tccgacgcgc tcgcgcgcgg gcaccgcatc gagatccgcg
     4081 gcttcggcag tttctccatc agccggcgcc cgccgcgcgt gggccgcaac ccgcgctccg
     4141 gcgagcaggt caccatccct gaaaagctgg tgccccactt caagccgggc aaggccctgc
     4201 gcgaagcggt cgacaaggaa ctgccggccg gcaacaagtc gtgatccctc cagggcgcct
     4261 tcgggcgcct tgttcattcc aggggcgcct ccgggcgccc ttgttcattc caggaatgcc
     4321 ttgcggcgcc cgggtcgaat cctgcggctc cgcttgtctg cgcgcaggat gacgacctgg
     4381 cgtctgccac gatgtccagt cgccgcatcc gcgtcgcggc cgtcgcgcat gcgcccgcgc
     4441 cccggcgcgt cacgcctgcg cgagcgcccg cacgtcgcgc gcgacctcgg ccatgaagcc
     4501 ttcgaactga tgcccgcagc cgggcaggcg gcggatggtc gcgtcgggga acagcactcc
     4561 gtaaagatcg gcatgcgacg gcggcaccgt cgtgtcggcc aggccgaagt acaggtgcag
     4621 cgtcaggcct cgcaggcgct cggggtcgac cgcggcgtcc aggtggaagc cgtccagctg
     4681 ccagccgccg cggcccacga atggcggcgc cagcaggatc acgccctgca gcgcggccag
     4741 ctcgtcgggc gggccctgtg ccagcaggtc cgccaccagc gcggcgcctg ccgagtgggc
     4801 gacgagaaaa gtcgcgcgcg tctgtcgcag cgttgtcgaa atgcggcgct tccagacgtc
     4861 gttgtcagga gcgtcctcgt gcggcatgtg cgggaacagg acccggaagg ccgggcccag
     4921 cgcccggctc aacgcatcgg acaggacgcg gtccgcctcg tgggcgccgg cgctggcgcc
     4981 ctggatgaac agcagttcga caggagctgg agacatgacg acgttttcgg gaaacacggg
     5041 tcgtgcaacg tcagcaagac cggcgcctgc tgacagaacg ttgtcagcag ccctcgcgtc
     5101 caatgcttcc aacgacaaca agcccccagg agagaacccc atgtggaccc acagcacgag
     5161 catcgccacc accgccactc cgcaggccct gtggcgcctg ttcgaggatg tcgccggctg
     5221 gacgaactgg aacgccggca tcgagcgcat cgcactgaac ggcccgttcg ccgccggttc
     5281 gaccttcgac atgaaggcgc ccggcatgga cacgttccgc agcacgctgc tcgatgtgcg
     5341 gcccaacgcc agcttcgtcg acgagacggt gctcgagggc acgcgcttcc tcgtctcgca
     5401 cgagctgcag cggctgccgt ccggcggcac gcgcgtcacc tatgcgatca cggtcgaggg
     5461 cccggaggcc gccacgctgg ggccgctggt gacggccgac ttcccggaag tgctggccgc
     5521 gctgaaggcg ctggccgagc gcgcgccggc gggggcgacc caatgacgcg caccttcgct
     5581 ccgccaggcc
//
LOCUS       DATCRO010000017         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_922821, whole genome shotgun sequence.
ACCESSION   DATCRO010000017 DATCRO010000000
VERSION     DATCRO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922821"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VIN75_00390"
     CDS             <1..763
                     /locus_tag="VIN75_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972642.1"
                     /translation="HVAGISWSPGRASIGMGMGQSMYAWMKDAFAQGASRADGTLATA
                     DVNFRQRSLLSFEDALLTAITVPRLDAASKESGAFDIEFAPARVRLAKGDGSDIRHAP
                     GGKQQPWVCSNFRFALGTLPCARVASIESFTWRCAAAPDAMGVVSPPAQHPLAAVVPD
                     LRISISLADYDAWSNAARAWFIDGRHLEGDEMDGVITLLTPDLQTPLGEIALGNVGFK
                     RFSQLPAADGSDALYRFTVDLYVERMSLRLVQYEG"
     gene            913..1338
                     /locus_tag="VIN75_00395"
     CDS             913..1338
                     /locus_tag="VIN75_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972643.1"
                     /translation="MRHLNKKLALAATATALIGAIGASVAQSADPNETDSTNTAQKVE
                     VNGSGQQIYFFRDQGNSPETDPAAHLMLIKHESEAVPVAETTTTTTVAQNTTTNNDAV
                     TTTMPAASDTSNTETSTDTTTTAAPVDNQPALAPKADRN"
     gene            complement(1505..1945)
                     /locus_tag="VIN75_00400"
     CDS             complement(1505..1945)
                     /locus_tag="VIN75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8972644.1"
                     /translation="MSPAARYDLVVLLHVTADIVFVAGLLAAALVLAALGTHDAAQLA
                     RERRLIGAMRGWHRKVTTPSLLLAWGLGVWLAVQAGWFHSGWLHVKLALVLALSALHG
                     VASGALRRASADAAAPPARAWRVLPLLALAAIAGVVWLALLKPF"
     gene            complement(1942..>3394)
                     /locus_tag="VIN75_00405"
     CDS             complement(1942..>3394)
                     /locus_tag="VIN75_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8972645.1"
                     /translation="GGPPDELIGWESLSLKGVKFAMTPSAKPRLDIGQVELDDLFARV
                     LVTEQGRLNLQDIGGNDATAAPPPGAASAPATAASAPLPIDIAIGGIKLVDARVDYAD
                     HFVKPNYSAALTELNGSLGAFSTLSRDMAPLQLHGRAEGTAILDISGQVNPLARPLAL
                     DVQAKATDLELAPLTPYAGKYAGYAIERGKLSMDVAYKIDAEGHLEARNQVILNQLTF
                     GDPIASPDATKLPVRLAVALLKDRNGVIDINLPISGSLNDPQFSVGGIVLKLIGNLLL
                     KAVTSPFALLTGGGGGGPDLSAIEFRPGTAVMTDASAASLDKVAKALADRPALQTTIT
                     GSADPQAEHDAWQREQVEAQLRTMARDEALRAGVPASAAASGAAALPAATRSTLLKTL
                     YKQTRIPDKPRNLVGMAKDVPDAEMEALLSRNVPVNDEAMRQQALARAIAVRDALVAR
                     GISTDRLFLAAPSLHAPGGDAWTPRASLSVAMP"
BASE COUNT          504 a         1213 c         1207 g          470 t
ORIGIN      
        1 gcacgtcgca ggcatctcct ggtcgccggg ccgcgcgagc atcggcatgg gcatggggca
       61 gtccatgtac gcgtggatga aggacgcctt cgcgcagggc gcatcgcgcg cggacggcac
      121 gctggccacc gccgacgtca acttccggca gcgctcgctg ctgagcttcg aggacgcgct
      181 gctgaccgcc atcacggtgc cgcggctgga cgcggccagc aaggagtcgg gcgcgttcga
      241 catcgagttc gcgccggcac gcgtgcgcct ggccaagggc gacggctcgg acatccgcca
      301 tgcgcctggc ggcaagcagc agccctgggt ctgctcgaac ttccggttcg cgctcggcac
      361 gttgccgtgc gcgcgcgtgg ccagcatcga gtccttcacg tggcgctgcg cggccgcccc
      421 ggacgcgatg ggcgtcgtgt cgccgccggc gcagcacccg ctggccgccg tcgtgcccga
      481 cctgcgcatc tcgatctcgc tggccgacta cgacgcgtgg tcgaacgccg cgcgcgcctg
      541 gttcatcgac ggccgccacc tcgaaggcga cgagatggac ggcgtgatca cgctgctgac
      601 gcccgacctg cagacgccgc tgggcgagat cgcgctgggc aatgtcgggt tcaagcgctt
      661 ctcgcagctg cccgcggccg acggcagcga cgcgctgtac cgcttcacgg tcgacctgta
      721 cgtggagcgg atgtcgctgc ggctcgtgca gtacgagggg tgaacgcagg ttgctcgaac
      781 gtcaagcgcg cgttgacgag gctttgtccg acaacgatcg tgctcgacag ggcattggtg
      841 ttgccgactc ggggttgcta ccgagcttcg actccacgaa gcaagggacc cccaccccaa
      901 ggagtacaca ccatgcgtca cctgaacaag aagctcgcgc tcgccgcgac cgccacggcg
      961 ctgatcggcg cgatcggcgc ctccgtcgcg cagagcgccg accccaatga gaccgactcg
     1021 acgaacacgg cccagaaggt cgaggtcaac ggctcgggcc agcagatcta cttcttccgc
     1081 gaccagggca actcgcccga gacggatccc gcggcgcacc tgatgctgat caagcacgag
     1141 tccgaagcgg tgccggtggc cgagaccacc acgaccacca ccgtggcgca gaacacgacg
     1201 acgaacaacg acgccgtgac gaccacgatg ccggccgcca gcgacaccag caacacggag
     1261 acgagcaccg acacgacgac gaccgcggcc ccggtcgaca accagccggc gctggcgccc
     1321 aaggccgacc gcaactgatc ggcttgattg gcggctcggc tcgtgcgatg ccgccggcac
     1381 ttccgatatt cggtagggcg ggtcggccgc agggatagcc cgccaagccg gtacatcgca
     1441 acgccggcga acggcgggtt acgcctgcgg ctaacccgcc ctacccggtt tgccgggcat
     1501 ggagtcagaa cggcttgagc agcgccagcc agacgacgcc tgcgatcgcg gccagcgcaa
     1561 gcagcggcag cacgcgccac gcgcgcgcgg gcggcgcggc cgcgtccgcg ctcgcgcgac
     1621 gcaacgcgcc cgacgccacg ccgtgcagcg cgctcagcgc cagcaccagc gcgagcttga
     1681 cgtgcagcca cccgctgtgg aaccagccgg cctgcacggc cagccacacg cccaagcccc
     1741 aggcgagcag cagcgacggc gtggtgacct tgcggtgcca tccacgcatc gcgccaatga
     1801 gccggcgctc gcgcgccagc tgcgccgcgt catgcgtgcc cagggccgcc agcaccagcg
     1861 ccgccgcgag caggccggcg acgaacacga tgtccgccgt cacgtgcagc aggacgacga
     1921 ggtcgtagcg cgcggccggg ctcatggcat cgcgaccgac agcgacgcgc gcggcgtcca
     1981 cgcgtcgccg ccgggcgcgt gcagcgacgg cgcggccagg aacaggcggt cggtggagat
     2041 gccccgcgcc accagcgcat cacgcaccgc gatcgcgcgc gcgagcgctt gctgccgcat
     2101 cgcctcgtcg ttgacgggca cgttcctgct cagcaacgcc tccatctcgg cgtcgggcac
     2161 gtccttggcc atgcccacga ggttgcgcgg cttgtcgggg atgcgcgtct gcttgtacag
     2221 ggtcttgagc agcgtcgagc gcgttgcggc cggcaacgcc gcggcgccgc tcgcggccgc
     2281 gctggccggc acgcccgcgc gcagcgcctc gtcgcgcgcc atcgtgcgca gctgcgcctc
     2341 gacctgctcg cgttgccagg cgtcgtgctc ggcctgcggg tcggcgctgc cggtgatcgt
     2401 cgtctgcagc gcggggcgat cggccagcgc cttggcgacc ttgtccagcg atgcggcgct
     2461 cgcgtcggtc atgacggccg tgcctggcct gaactcgatc gcgctcaggt cggggccgcc
     2521 gcctccaccg ccggtcagca gcgcgaacgg cgaggtcacg gccttcagca acaggttgcc
     2581 gatcagcttg agcacgatgc cgccgacgct gaactgcggg tcgttgaggc tgccgctgat
     2641 gggcaggttg atgtcgatga cgccgttgcg gtccttcagc agcgccaccg cgaggcgcac
     2701 cggcagcttg gtcgcgtcgg gcgaggcgat cgggtcgccg aaggtcagct ggttcaggat
     2761 gacctggttg cgtgcctcga ggtggccttc ggcgtcgatc ttgtaggcga cgtccatgct
     2821 cagcttgccg cgctcgatcg cgtagccggc gtacttgccg gcgtagggcg tgagcggcgc
     2881 gagctcgagg tcggtggcct tggcctggac gtcgagcgcc agcgggcggg cgagcgggtt
     2941 cacctggccg gagatgtcca ggatcgcggt gccctccgcg cggccgtgca gctgcagcgg
     3001 cgccatgtcg cgcgagagcg tgctgaacgc gccgagcgag ccgttgagct ccgtcagcgc
     3061 cgcactgtag ttgggcttga cgaagtgatc ggcgtagtcg acgcgcgcgt cgacgagctt
     3121 gatgccaccg atggcgatgt cgatcggcag cggggcggac gccgccgtcg ccggtgcgga
     3181 cgccgcgccg ggcggcggcg ccgcggtggc gtcgttgccg ccgatgtcct gcaggttcag
     3241 ccggccctgc tcggtgacca gcacgcgcgc gaacaggtcg tcgagttcca cctggccgat
     3301 gtccagccgg ggcttggcgg acggcgtcat ggcgaacttc acgcccttga gcgacagcga
     3361 ctcccagccg atcaattcat ccggcggccc gccg
//
LOCUS       DATCRO010000018         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_258987, whole genome shotgun sequence.
ACCESSION   DATCRO010000018 DATCRO010000000
VERSION     DATCRO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258987"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VIN75_00410"
     CDS             <1..1187
                     /locus_tag="VIN75_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY8972646.1"
                     /translation="LKLPTTIGVMATSLVLSLALVGLDAAGLAGGLHANEERLVRSID
                     FSQLLMQGMLSLLLFAGALHVDLRALRDYRWQVGVLAFVGTALSTVLVGLALWRAMGA
                     LGLELPLHACLLFGALISPTDPIAVIGMLKSAGAPRNLEVVIAGESLFNDGVGVVLFV
                     LLLEMAQDGGAPTLSHGAALLAREAGGGLLFGGALGFVLCRLLRGVQAHAVAVLLTLA
                     AVIGGYTLANDLHVSGPLAMVVVGLMVGHAGREGRLHEETQHHLGVFWELIDDVLNAV
                     LFVLVGMEVVTIRFPVGPGAAIGAGLAAIAIALAARWLTVGLPVALARRASRLPAGAG
                     GLLTWGGLRGGLSLALALALPAGPWRDTLVALTYAVVAFSILVQGLTFGRLVRARLRS
                     DA"
     gene            1293..1679
                     /locus_tag="VIN75_00415"
     CDS             1293..1679
                     /locus_tag="VIN75_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972647.1"
                     /translation="MDLKVPITISDELGDDIIESTGLLDLASGEIQRVEYEDWDLAAR
                     GLPADSEDYEFTVGTLSNNGKDVEFKVDVNKVTGQYSVSASELLEIKVRAAALFAGIS
                     GKDMLRNADAKAATPPAGGKGRKHLH"
     gene            complement(1775..1951)
                     /locus_tag="VIN75_00420"
     CDS             complement(1775..1951)
                     /locus_tag="VIN75_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972648.1"
                     /translation="MNSKHKLVRWGALALLVTLMSGCCVAPWGGRYYGGGYYHGGGYG
                     GGGGYEHGGGGWRH"
     gene            2249..2488
                     /locus_tag="VIN75_00425"
     CDS             2249..2488
                     /locus_tag="VIN75_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972649.1"
                     /translation="MKNGLAIAIAGLALASMSGCIVAPARPAYYGEGGYVAPAGVVYV
                     APTYAAPGPEWRWEYHATYGWGWHHPRYGWHRGWH"
     gene            complement(2660..3085)
                     /locus_tag="VIN75_00430"
     CDS             complement(2660..3085)
                     /locus_tag="VIN75_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972650.1"
                     /translation="MTLRHSFLAGTILALACAGAQAQGHDHDRDHGGPPPAHGAPGKP
                     SPATGRTGPGWSGAHARGYVVVAAPPGADASATAPPPPPPAPDPIFYPRNGQGAEQTE
                     ADRRDCNSWATTQPNAMADAKVFQRATEACMDGRGYTMR"
     gene            complement(3204..>3913)
                     /locus_tag="VIN75_00435"
     CDS             complement(3204..>3913)
                     /locus_tag="VIN75_00435"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEY8972651.1"
                     /translation="PQDTGGGKAAPLAPVVTQPFVDDGSNGDAQAGDGTFTTRLDPTA
                     EGFGNFAGLIRTELVVQSGSQQGYVAFDVVYTPQSPATWTGGVHDAMRDGSLAFLLGV
                     DVIQPGRYVITGRVDDATGKPVALVTFNDELGAGARQVPLQVYGRLVRDLKPALPLTL
                     HDVEGFLLKPDAYPDRALMPSLDGPVATSRKYAAAQFSDAPFSSDETRRYLAEYGKDV
                     TQAQQQVAQLQGPPAKP"
BASE COUNT          509 a         1336 c         1457 g          611 t
ORIGIN      
        1 ggctcaagct gccgaccacc atcggcgtga tggccacgtc gctcgtcctg tcgctcgcgc
       61 tggtgggcct ggacgcggcc ggcctggccg ggggcctgca cgcgaacgag gagcggcttg
      121 tccgctcgat cgatttttcc cagctgctga tgcagggcat gttgtcgctg ctgctgttcg
      181 ccggcgcgct gcacgtcgac ctgagggcgt tgcgggacta ccgctggcag gtgggcgtgc
      241 tggcgttcgt cggcaccgcg ctgtcgacgg tgctggtcgg actcgcgctg tggcgggcca
      301 tgggtgcgct cggcctggag ctgccactgc acgcatgcct gcttttcggc gcgctgatct
      361 cgcccacgga cccgatcgcc gtcatcggca tgttgaagtc ggccggggcg ccgcgcaacc
      421 tcgaagtcgt gatcgcaggc gaaagcctgt tcaacgacgg cgtgggcgtg gtgctgttcg
      481 tgctgctcct ggagatggcg caggatggcg gcgcgccgac cctgtcccat ggggccgcgc
      541 tgctcgcgcg ggaggccggg ggcggcctgt tgttcggagg ggcactgggc ttcgtgctgt
      601 gccggctcct gcgcggcgtg caggcccatg ccgtggccgt gctgctgacg ctggcggccg
      661 tgatcggcgg ctacacgctc gccaacgacc tccatgtgtc ggggccgctc gccatggtgg
      721 tcgtcggctt gatggtgggt cacgccggcc gggagggtcg cctgcacgaa gagacgcagc
      781 accacctggg cgtgttctgg gagctgatcg acgacgtcct caatgcggtg cttttcgtgc
      841 tggtgggcat ggaggtcgtc acgatccgat tccccgtcgg accgggtgcc gcgatcgggg
      901 ccgggctggc ggcgatcgcc attgccctgg ccgcgcgctg gctgacggtg ggcctgcccg
      961 tcgcgctcgc gcgtcgcgcg tcacgcctgc ccgcgggcgc gggcgggctg ctgacctggg
     1021 gcggcctgcg cggcggcctg tcgctcgcgc tggcgctggc cttgcccgcc gggccgtggc
     1081 gcgacacgct cgtggcgctg acctacgccg tcgtcgcgtt ctcgatcctg gtccagggcc
     1141 tgaccttcgg ccggctggtg cgcgcccggc tgcgaagcga cgcctgaacg ccacatgcgg
     1201 cggcgcagca cggcgcgcgg gcatgccgtt cgctgtcgat ccagttcggg cgtgggacaa
     1261 tctcgtcctc gacaacagca caaggccaag ccgtggacct gaaggtaccg atcacgattt
     1321 ccgatgaact gggcgacgac atcatcgagt cgaccggtct gctcgacctg gcgagcgggg
     1381 agatccagcg ggtcgagtac gaggactggg acctcgccgc ccgcggcctg cccgccgaca
     1441 gcgaggacta cgagttcacg gtcggcacgc tgtcgaacaa cggcaaggac gtcgagttca
     1501 aggtcgacgt caacaaggtc acggggcagt actcggtgag cgcgagcgag ctgctcgaga
     1561 tcaaggtgcg cgcggccgcg ctgttcgccg gcatcagcgg caaggacatg ctgcgcaacg
     1621 ccgacgccaa ggcggccacg ccgccggccg gcggcaaggg ccgaaagcac ctgcactgaa
     1681 gcggcgcgag cccgtttcca tggacgaaag gccgcgatcg cggccttcgt cgcttccggg
     1741 agcgatcgtt ccgcgccgga gcgcggacga gcgatcagtg gcgccagccg ccgccgccgt
     1801 gctcgtagcc gccgccacca ccgtagccgc cgccatggta gtagccgcca ccgtagtagc
     1861 gaccgcccca cggggccacg cagcagccgc tcatcagcgt cacgagcagg gccagtgcgc
     1921 cccagcgcac gagcttgtgt ttcgagttca tccggttctc ctgcaaaagg ctgctccagt
     1981 gccgccttgt gctattaaac gccgcttgtc atcgatcgat gacaagctcc gcgtttccgt
     2041 ttgcaaagcc gcgtaaaccg tcgagcagcg cttcatgggt gcggcgcgta aagatggccg
     2101 cgctgctcga ctgtcggccg gtgtcagggt caacgcatgt gtcgaaacgg ccgttgtcca
     2161 ggcacagggc gcgcgacttg ccgtggtgac cccggagggg tcgacgaagc ggtcgcggac
     2221 accccgcgaa cccgaaaagg aagcaatcat gaagaacggt cttgccattg ccatcgccgg
     2281 cctcgcgctg gcgtccatgt ccggctgcat cgtcgcgccg gcacgacccg cctactacgg
     2341 cgagggcggc tacgtcgcgc cggccggcgt cgtgtacgtg gcgccgacct acgccgcgcc
     2401 cggcccggaa tggcgctggg aataccacgc cacctacggc tggggctggc accacccgcg
     2461 atacggctgg catcgcggct ggcattgaac cggccgcacg gcgcacggct cgcccgggca
     2521 ggcgagcccg cgggcgggcc gcggtgcccg cgcgtcacgc gcgcgtgacc gccggcgcgg
     2581 cgccagggac tcgcggcgcc ggccgcacca acgacaacgg gctccgatcg gagcccgttt
     2641 cgcttggcgc atcgcccgtt cagcgcatcg tgtagccgcg cccgtccatg caggcttcgg
     2701 tggcgcgctg gaagaccttg gcgtccgcca tcgcgttggg ctgcgtcgtg gcccagctgt
     2761 tgcaatcgcg gcgatcggcc tccgtctgtt cggcgccctg gccgttgcgc ggatagaaga
     2821 tcgggtccgg cgcgggcggc ggtggcggcg gggcggtggc ggacgcgtcg gcgcccggcg
     2881 gcgccgcgac cacgacgtag ccgcgcgcgt gggcgccgct ccagcccggg cccgtgcgac
     2941 ccgttgcagg gctgggcttg cccggcgcgc cgtgcgcggg cggcggtccg ccgtggtcgc
     3001 gatcatggtc gtggccttgc gcctgggcgc ccgcgcacgc gagcgccagg atcgtgccgg
     3061 cgaggaagga atggcggagg gtcatggcgg tgcggtgggt cgcggcgcat gccgccggcc
     3121 cattgtcgag aagccgtgtc agctgccgat tgcatcatgt caacggcgtg tgaagcctgg
     3181 tcgcagccaa ggccgacacg tcctcagggc ttggcgggcg ggccctgcag ttgcgccacc
     3241 tgctgctgcg cctgcgtcac gtccttgccg tattcggcca ggtagcgccg cgtctcgtcg
     3301 ctggagaacg gcgcgtcgct gaactgggcc gccgcgtact tgcgcgaggt cgcgacgggg
     3361 ccgtcgagcg agggcatcag cgcgcgatcg gggtaggcgt cgggcttgag caggaagccc
     3421 tcgacgtcgt gcagtgtcag cggcagcgcc ggcttcaggt cgcgcaccag gcggccgtag
     3481 acctgcaacg gcacctggcg cgcgcccgcg cccagttcgt cgttgaaggt cacgagcgcc
     3541 accggcttgc cggtcgcgtc gtcgacgcgg cccgtgatga cgtagcggcc gggctggatc
     3601 acgtcgacgc ccagcaggaa ggccagcgag ccgtcgcgca tcgcgtcatg cacgccgccg
     3661 gtccaggttg ccggggattg cggcgtgtag accacgtcga acgccacgta gccctgctgg
     3721 ctgccggact gcaccaccag ctcggtgcgg atcagcccgg caaagttgcc gaagccttcg
     3781 gccgtggggt ccagccgcgt ggtgaaggtg ccgtcgccgg cctgggcatc gccgttggag
     3841 ccgtcgtcga cgaagggctg cgtgacgacg ggcgcgagcg gggccgcctt gccgccgccg
     3901 gtgtcctgcg ggc
//
LOCUS       DATCRO010000019         9872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_315240, whole genome shotgun sequence.
ACCESSION   DATCRO010000019 DATCRO010000000
VERSION     DATCRO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315240"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..998
                     /locus_tag="VIN75_00440"
     CDS             180..998
                     /locus_tag="VIN75_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY8972652.1"
                     /translation="MAQAHDNEFSRIALACVAVAALALAGCGQKKTELGEGGSVVSGS
                     AGPAGAQNASHELVRCDAPVATMALVENPNGYTMAGGYNLPSSPLPLVRLIAQQSGCF
                     RIVDRAAGLHATVQEQQLKDAGILGDAVKVRKGQGYAAQYTITPSLTFSERDAGREVG
                     GVLAHIPVLNKFVGLADQVKLKEAQVALLLTDNETTEQLAAATGSVRVTDLGMAGLML
                     GRAGGAAGAGWSNTNEGKVIAAAFLDAHNQLVTQLRMLQAKDLPPPVPTKKRAG"
     gene            1007..1339
                     /locus_tag="VIN75_00445"
     CDS             1007..1339
                     /locus_tag="VIN75_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972653.1"
                     /translation="MARASARATALLLAELRGSMRVQALTLRAGTVLLLCFPLSAAIG
                     SLLHGLGFYPGWLQALVEHRDALLFLAMLFSQVAFCDSIVRHRTQWQYLGEIAVAIAL
                     GYLTSGLA"
     gene            1451..3079
                     /locus_tag="VIN75_00450"
     CDS             1451..3079
                     /locus_tag="VIN75_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY8972654.1"
                     /translation="MSAELATQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGS
                     VKARKAARHATSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSEDTYRVL
                     TAVDAALMVIDAANGVEPQTRRLLQVCRARNTPILTFVNKMDREVQEPLALMDEIERE
                     LGMSVVPFTWPVGMGKLFGGVVDLRKKQMRVFAPGEDRAGGDEEVITDLDNPGNADRF
                     GMGWGQAQGEIELLGAAAPEFDEEAFLEGRQTPMFFGSAINNFGVQEVLDALVNLAPA
                     PGARPAMQREVQPDEGKFTGVVFKIQANMDPSHRDRIAFLRVASGHFERGMRLKVVRS
                     GKELRPNTVVSFLSQRRELLDEAFAGDIIGIPNHGVLQLGDTLTEGEQLQFTGLPFFA
                     PELFRSVEVADPLKTKQLRAGLTQLGEEGAIQVFRPVLGTVLLLGAVGQLQFEVVAHR
                     LEHEYGVRARITPARYALARWVTCEPDDGGDKELQRFIDANAHRIAYDAVDAPAILVE
                     YAPELRAVEANWPKIKFHALREHAGLIFQKRLDD"
     gene            3131..3757
                     /gene="dsbG"
                     /locus_tag="VIN75_00455"
     CDS             3131..3757
                     /gene="dsbG"
                     /locus_tag="VIN75_00455"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEY8972655.1"
                     /translation="MTRQFPLICLAGAALLALACAAAPAASTWSAATEHRNALPGEWE
                     QLEHGRWIADGRDDAPRKVYVFLDANCKYCTKLWSDARPWVNAGKVQLRHIMVGVIAP
                     SSPGKAATLLADPDPSRRLAAFEQAHAFGVVRMMSGGPHQALEDPNLAPMDPVPEALA
                     RTLSDNEDLMHGFGLMGTPGIVFRSRDGHVVAKPGLSPEELPQVLGPL"
     gene            3786..4694
                     /gene="ubiA"
                     /locus_tag="VIN75_00460"
     CDS             3786..4694
                     /gene="ubiA"
                     /locus_tag="VIN75_00460"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY8972656.1"
                     /translation="MASRVSLYLDLIRWNRPAGWLLLLWPTLAALWIACDGFPGWHLL
                     AVFTLGTILMRSAGCCINDVADRNFDKHVKRTAQRPVTSGALSVKAALTFGAILALLA
                     FCLVLTTNPPTILLSFAALAITLVYPYAKRFVSMPQAVLGVAFSFGIPMAFCAATGAR
                     DWSLSAIPAAVPALAWWLLLGNLFWVLAYDTEYAMVDRDDDLKIGIRTSAITLGRLDV
                     AGVMAFYAVYLLSWLYLGLKLGLGPTYAVGIVVAGLQAGWHFTLIWGRTREGCFRAFR
                     ANHWLGAAVFAGVVLSFAVNRHGAPG"
     gene            4691..5185
                     /locus_tag="VIN75_00465"
     CDS             4691..5185
                     /locus_tag="VIN75_00465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8972657.1"
                     /translation="MSAVTLRAVERAQWPVVENLLQFYHYELSAWYPIAFAADGRYAI
                     PSKDDYLAQPGTQAWLILVDDELAGFAVIDDELVDTRRDLNLGYLFVGRRFRGRGVGA
                     AAFSGLLARYPGQWELYYLARNAAAAAFWPKAFARAGLAQVDVTDEHLFGEDCVMNRF
                     VAAP"
     gene            complement(5190..5573)
                     /locus_tag="VIN75_00470"
     CDS             complement(5190..5573)
                     /locus_tag="VIN75_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972658.1"
                     /translation="MSDEKLEFKWEWPAGRPIATKVLVHVDAIDKVSSGLFGLGKSPS
                     VEGYLPDARTLRASVVAGPQELAGRRLTLNLPGPEASKLVVGGNAGVGLIAGRDKGAG
                     IAVCVAPAPEGSSTQWLSSWNCPTN"
     gene            complement(5675..6400)
                     /locus_tag="VIN75_00475"
     CDS             complement(5675..6400)
                     /locus_tag="VIN75_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972659.1"
                     /translation="MTRQARALQFRGWPSITAMARLRAVFSSLVRSMATTNYNFGGDR
                     VALIVRGKHSADHEPSVMAQHADCALSKGAPVGFFGEAGGGGSSGRAPSSIGASGRGS
                     TGKSNSIGLGMAGVVYDFDKLRQNRPFYVELAQAKGYNLVSTVLFVQVTASEAKAFDD
                     AWAGLTKSPGSFQLLGWNCSTHASQAFRASGILSGGIPGLDTPDNLYKQLCKERAGKV
                     VSVSGYVGFARSGSGFMMTVDTP"
     gene            complement(6397..7263)
                     /gene="proC"
                     /locus_tag="VIN75_00480"
     CDS             complement(6397..7263)
                     /gene="proC"
                     /locus_tag="VIN75_00480"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8972660.1"
                     /translation="MMNPTPPTAPSQGPCAASPAPLLAFVGGGNMASAIVGGLVAAGH
                     PPGRVVVVEPYAPQADIVREKFGVRVEAAGGAALAGADIVVWAVKPQVFVEAAAPCAA
                     HVARALHLSVMAGIRSDAIARATGSERVVRAMPNTPALIGEGIAGLFARAAVTAGDRA
                     AIERVLAPTGETLWVARESDLDAVTALSGSGPAYVFFFIEAMMQAAADMGLSPEQGRR
                     LAQATFKGATSLARASDEAPETLRARVTSKGGTTHAAITAMEAAGVKPAIVAALRAAQ
                     ARAKELGDEFGG"
     gene            7500..8771
                     /locus_tag="VIN75_00485"
     CDS             7500..8771
                     /locus_tag="VIN75_00485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8972661.1"
                     /translation="MSNLSYVAPTDDSPWGTYLSQVDRVVPYLGNLGRWAETLKRPKR
                     ALIVDVPIELDNGTVAHFEGFRVQHNLSRGPGKGGVRYHPDVTLEEVMALSAWMTVKC
                     AAVNLPFGGAKGGIRVDPKKLSQPELERMTRRYTSEIGLIIGPQKDIPAPDVNTNGQI
                     MAWMMDTYSMNTGSTSTGVVTGKPIHLGGSAGRVKATGRGVFVTGREAARRIGLDLNG
                     ARVAVQGFGNVGGSAAELFGQAGAKIVAAQDHTGTIFNANGFDLSELMPHVARTGGVA
                     GFKGADTMKAEEFWDVDCEIMIPAALEGQISESRAKRIKAKMVLEGANGPTTPAADDI
                     LAERKILVVPDVICNAGGVTVSYFEWVQDFSSFFWSEDEINVRLDKIMVDALKKIWET
                     ADRHKITLRTATFAVACERILIAREERGLYP"
     gene            complement(8840..>9872)
                     /locus_tag="VIN75_00490"
     CDS             complement(8840..>9872)
                     /locus_tag="VIN75_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972662.1"
                     /translation="QEEPELLQQALKRELIVIWNLRSSRLVMGTGADKHMRLGARRNL
                     PSYIPTAHFATAFIDFVARGPAGPGDAPDTAPPGALTIELLRRQAAQLPDHLARTVIA
                     GIDYANGDIAKVRQAVEQWFDGAMDRASGWYKRRTQALLFTIGLLTAVVLNVDALHIL
                     HRLTHDKTFRDVVVSRAAAAKPPASAGAGDEHAAMTLARAELDAVTMPIGWESPTTPG
                     AESQLWPTQFCSASADDTGALSETCTFGRNPVYAVVRMGLGWLVTALAVMLGAPFWFD
                     VLNRIMVIRSTVKPHEKSPEEPSQDGGSDRVQRVVVSAEPSPPPPTPPAAPDDGTPDA
                     TDAAPRPAG"
BASE COUNT         1448 a         3502 c         3457 g         1465 t
ORIGIN      
        1 agaaggacgc gtgggtcgac cacgcgctca aggtcgtcgg ggacgcgctg ggcgacgccg
       61 cgacggcctg atttgtttca gggtggcaac aagcgtgggg ggcgcgcctg cccgcgggca
      121 acgaggcccg tggcgctggc atgatcccgc ctttcccgat gagtggagag gttctggaca
      181 tggcgcaagc ccacgacaac gagttttcgc gcatcgcgct ggcctgcgtc gccgtcgcgg
      241 cgctggccct ggccggctgc ggccagaaga agacggagct gggcgaaggc ggctcggtcg
      301 tgagcggctc ggccggcccg gcgggtgcgc agaacgcgag ccacgagctg gtcaggtgcg
      361 acgcccccgt cgcgacgatg gcgctggtcg agaacccgaa cggctacacc atggccggcg
      421 gctacaacct gccgtcgtcg ccgctgccgc tcgtgcgcct gatcgcccag cagagcgggt
      481 gcttccgcat cgtcgaccgc gccgcggggc tgcacgcgac ggtgcaggag cagcagctca
      541 aggacgccgg catcctgggc gacgccgtca aggtgcgcaa gggccagggc tatgccgcgc
      601 agtacacgat cacgcccagc ctcacgttca gcgagcgcga cgcgggccgc gaggtgggcg
      661 gcgtgctcgc gcacatcccg gtgctcaaca agttcgtcgg cctggccgac caggtcaagc
      721 tcaaggaggc gcaggtcgcg cttttgctga ccgacaacga gaccaccgag cagctggccg
      781 ccgccaccgg ctcggtgcgc gtgaccgacc tcggcatggc cggcctgatg ctgggccgcg
      841 cgggcggcgc cgccggcgcg ggctggagca acacgaacga aggcaaggtg atcgccgcgg
      901 ccttcctcga tgcgcacaac cagctcgtga cgcagctgcg gatgctgcag gccaaggacc
      961 tgccgccgcc ggtgcccacg aagaagcgcg cgggctgagg ccacccgtgg cgcgcgcatc
     1021 ggccagggcc acggcactgc tcctggccga gctgcgcggc tcgatgcgcg tgcaggcgct
     1081 gacgctgcgc gccggcacgg tgctgctgct gtgcttcccg ctgtcggcgg cgatcggctc
     1141 gctgctgcac ggcctgggct tctaccccgg ctggctgcag gcgctggtcg agcatcgcga
     1201 cgcgctgctg ttcctggcca tgctgttctc gcaggtggcg ttctgcgact cgatcgtccg
     1261 gcaccggacg caatggcagt acctgggcga gatcgcggtg gcgatcgcgc tcggatacct
     1321 gacgagcggg ctggcctgaa agccgccgcg ccaggccccg gcggcgtggg agcggccgac
     1381 cggcgataat cgcggactcc ccctttcgca tgaagcgtcc gcccgtcccg ccgggccggc
     1441 gcacacgacc atgtccgccg aactcgccac ccaggtgcgc cgccgccgca ccttcgccat
     1501 catttcccac cccgacgcgg gcaagaccac gctcacggaa aagctgctgc tgttctcggg
     1561 cgcgatccag atcgccggtt cggtcaaggc gcgcaaggcg gcgcggcatg cgacgtccga
     1621 ctggatggag atcgagaagc agcgcggcat ctcggtggcg tcgtcggtga tgcagatgga
     1681 gtaccgcgac tgcgtcatca acctgctcga caccccgggc caccaggact tctcggaaga
     1741 cacctaccgc gtgctgaccg ccgtcgacgc ggcgctgatg gtgatcgacg cggccaacgg
     1801 cgtcgagccg cagacgcgcc gcctgctgca ggtctgccgc gcgcgcaaca cgcccatcct
     1861 cacgttcgtc aacaagatgg accgcgaggt gcaggagccg ctggcgctga tggacgagat
     1921 cgagcgcgag ctgggcatga gcgtcgtgcc gttcacctgg ccggtgggca tgggcaagct
     1981 gttcggcggc gtcgtcgacc tgcgcaagaa gcagatgcgc gtgttcgcgc cgggcgagga
     2041 ccgcgccggc ggcgacgagg aggtcatcac cgacctggac aaccccggca acgccgaccg
     2101 cttcggcatg ggctggggcc aggcgcaggg cgagatcgag ctgctgggcg ccgccgcgcc
     2161 cgagttcgac gaggaggcct tcctcgaggg caggcagacg ccgatgttct tcggctcggc
     2221 catcaacaac ttcggcgtgc aggaagtgct ggacgccctc gtgaacctgg cgcccgcgcc
     2281 cggcgcgcgc cccgcgatgc agcgtgaggt ccagcccgac gagggcaagt tcaccggcgt
     2341 cgtgttcaag atccaggcca acatggaccc gtcgcaccgc gaccgcatcg cgttcctgcg
     2401 cgtggcctcg ggccacttcg agcgtggcat gcggctgaag gtggtgcgct ccggcaagga
     2461 gctgcggccg aacaccgtcg tgtccttcct gtcgcagcgc cgcgaactcc tggacgaggc
     2521 cttcgccggc gacatcatcg gcatccccaa ccacggcgtg ctgcagctgg gggacacgct
     2581 caccgagggc gagcaactgc agttcaccgg gcttcccttc ttcgcgccgg agctgttccg
     2641 cagcgttgaa gtggccgatc cgctcaagac caagcagctg cgcgcgggcc tgacgcagct
     2701 gggcgaagaa ggcgcgatcc aggtgttccg gccggtactg ggcacggtat tgctgttggg
     2761 cgccgtcggc cagctgcagt tcgaagtggt ggcgcaccgc ctcgagcacg agtacggcgt
     2821 gcgcgcccgc atcacgcccg cgcgctacgc gctcgcgcgc tgggtcacct gcgagccgga
     2881 cgacggcggc gacaaggagc tgcagcgctt catcgacgcc aacgcgcacc gcatcgccta
     2941 cgacgcggtc gacgccccgg ccatcctggt cgaatacgcg cccgagctgc gcgccgtcga
     3001 ggccaactgg cccaagatca agttccatgc gctgcgcgag cacgcgggcc tgatcttcca
     3061 gaagcgcctg gacgactgaa cctttccggg cgggccgcgt ctgacggtgt ccctccactg
     3121 ccgacgcacc atgacccgcc agttcccgct gatctgcctc gccggcgccg ccctgctggc
     3181 gctggcctgc gccgcggcgc ccgccgcctc gacatggagc gccgccaccg agcaccgcaa
     3241 cgccttgccc ggcgagtggg agcagctcga gcacggccgc tggatcgccg acggccgcga
     3301 cgacgcgccg cgcaaggtct acgtcttcct cgacgcgaac tgcaagtact gcacgaagtt
     3361 gtggtccgat gcgcgcccct gggtgaacgc cggaaaggtg caactgcgcc acatcatggt
     3421 gggcgtcatc gcccccagca gcccgggcaa ggcggccacg ctgctggccg atcccgatcc
     3481 gtccaggcgt ctggccgcct tcgagcaggc gcatgcgttc ggcgtcgtgc gcatgatgtc
     3541 cggcggcccg caccaggcgc tggaggatcc gaacctcgcg ccgatggatc ccgtccccga
     3601 ggcgctggcg cgcacgctgt ccgacaacga ggacctgatg cacggcttcg gcctgatggg
     3661 cacgccgggc atcgtcttcc gctccaggga cggccacgtc gtggccaagc cgggcctgtc
     3721 gccggaggag ctgccgcagg tgctggggcc gctgtaggcg tcgcggcctg ggcgagaatc
     3781 gcgccatggc ctcgcgcgtc tccctctatc tcgacctgat ccgctggaac cgccctgccg
     3841 gctggctgct gctgctgtgg cccacgctcg ccgcgctgtg gatcgcctgc gacggctttc
     3901 ccggctggca cctgctggcg gtgttcacgc tcggcacgat cctgatgcgc agcgcaggct
     3961 gctgcatcaa cgacgtcgcc gaccgcaact tcgacaagca cgtcaagcgc accgcgcagc
     4021 ggcccgtcac cagcggcgcg ctgtcggtga aggcggcgct gaccttcggc gcgatcctcg
     4081 cgctgctcgc gttctgcctc gtgctcacca cgaacccgcc gacgatcctg ctgtcgttcg
     4141 cggcgctcgc gatcacgctc gtctatccgt acgcgaagcg cttcgtgtcg atgccccagg
     4201 cggtgctggg cgtcgcgttc agcttcggca tcccgatggc gttctgcgcg gcgaccggcg
     4261 cacgcgactg gtcgctgtcg gccattcccg ctgcggtgcc ggcgctcgcc tggtggctgc
     4321 tgctgggcaa cctgttctgg gtgctcgcct acgacaccga gtacgcgatg gtcgatcgcg
     4381 acgacgatct gaagatcggc atccgcacct ccgcgatcac gctgggccgc ctcgacgtcg
     4441 ccggcgtgat ggcgttctac gccgtctacc tgctgtcctg gctgtacctg ggcctgaagc
     4501 tgggcctggg gccgacgtac gctgtgggca tcgtggtcgc gggcctgcag gcgggctggc
     4561 acttcacgct gatctggggc cgcacgcgcg agggctgctt ccgcgccttc cgcgccaacc
     4621 actggctggg cgcggcggtg ttcgcgggcg tggtgctgtc gttcgcggtc aaccggcacg
     4681 gagcgccggg atgagcgccg tcacgctgcg tgccgtcgaa cgcgcgcagt ggcccgtggt
     4741 cgagaacctc ctgcagttct accactacga gctgagcgcc tggtacccga tcgccttcgc
     4801 agcggacggc cgctacgcga tcccttccaa ggacgactac ctcgcccagc ccggcaccca
     4861 ggcgtggctg atcctggtgg acgacgagct cgccggcttc gcggtgatcg acgacgaact
     4921 ggtcgacacg cggcgcgacc tcaacctggg ctacttgttc gtcggccggc gcttccgcgg
     4981 ccgcggcgtc ggcgcggcgg cgttcagcgg gctgctggcg cgctatccgg gacagtggga
     5041 gctgtactac ctggcccgca acgcggccgc cgcggccttc tggcccaagg cgttcgcgcg
     5101 cgccggcctg gcgcaggtcg atgtgaccga cgagcacctg ttcggcgagg actgcgtgat
     5161 gaaccggttt gtcgccgcgc cttgacggct cagttggtcg gacagttcca gctggacagc
     5221 cattgggtgc tgctgccctc cggcgcgggc gccacgcaga cggcgatccc cgcgcccttg
     5281 tcccgcccgg cgatcagccc cacgccggca ttgccgccga cgacgagctt gctcgcctcg
     5341 gggccgggca ggttcagcgt cagccgccgc ccggccagct cctgcggccc ggcgacgacc
     5401 gacgcgcgca gcgtgcgcgc atcgggcagg tagccctcga cggacggcga cttgcccagg
     5461 ccgaacaggc cgctcgacac cttgtcgatc gcgtcgacgt gcacgagcac cttcgtcgcg
     5521 atcggccggc cggccggcca ttcccatttg aactcgagct tctcgtcgga catggcgttg
     5581 catcccaggt gtgcggtgag gacgccggca acgaggccgg gcgtcgcaag acgggtgaat
     5641 cggcggcgat tgaggggcat cgggtctggc gaagtcacgg ggtgtccacg gtcatcataa
     5701 aaccgctgcc ggaacgagcg aacccgacgt agccgctgac gctgacgacc ttgccggcgc
     5761 gttccttgca cagctgcttg tagaggttgt cgggcgtatc caggcccgga atgccgccgg
     5821 acaggatgcc cgacgcgcga aacgcctgcg aagcgtgcgt cgaacagttc cagccgagga
     5881 gctggaacga tccgggcgac ttggtcaggc ctgcccaggc gtcgtcgaac gccttcgcct
     5941 cgctggccgt cacttgcacg aacagcaccg tggaaacgag gttgtagccc ttggcctgcg
     6001 cgagttcgac gtagaacggg cggttctggc gcagcttgtc gaagtcgtag acgaccccgg
     6061 ccatgcccag gccgatgctg ttggacttgc cggtgctgcc gcggccgctg gccccgatgc
     6121 tcgatggcgc gcgtccgctg gacccaccgc caccggcttc gccgaagaag cccacgggcg
     6181 cgcccttgga caacgcgcag tcggcgtgtt gcgccatcac cgacggctcg tggtcggcgg
     6241 aatgcttgcc gcggacgatc agggcaacgc ggtcaccgcc gaagttgtag ttggttgttg
     6301 ccatggagcg aacgaggctg ctgaagacgg cgcgcagtct cgccatggcc gtgatcgatg
     6361 gccatcccct gaactgaagt gcgcgcgcct gcctcgtcaa ccgccgaatt cgtcgcccag
     6421 ttccttcgcc cgcgcctgcg ccgcgcgcag ggccgcgacg atcgccggct tcacgccggc
     6481 cgcctccatc gcggtgatcg cggcgtgggt ggtgcccccc ttggacgtga cacgtgcgcg
     6541 cagcgtctcc ggcgcctcgt cgctggcccg cgccagcgag gtcgcgccct tgaacgtcgc
     6601 ctgcgccagg cggcggccct gctcggggct caggcccatg tcggcggcag cctgcatcat
     6661 ggcctcgatg aagaagaaga cgtaggccgg gccggagccc gacagcgcgg tgacggcgtc
     6721 caggtcgctc tcccgcgcca cccacagcgt ctcgccggtg ggcgcgagca cgcgctcgat
     6781 cgcggcgcgg tcgccggccg tgacggcggc gcgtgcgaac aggcccgcga tgccttcgcc
     6841 gatcagcgcc ggcgtgttgg gcatggcgcg cacgacgcgc tcggatcccg tggcgcgcgc
     6901 gatcgcgtcg ctgcggatgc cggccatcac cgacaggtgc agcgcgcggg cgacatgcgc
     6961 ggcgcacggc gccgcggcct cgacgaacac ctgcggcttg acggcccaca cgacgatgtc
     7021 cgcgccggcc agcgccgcgc cgccggcggc ctccacgcgc acgccaaatt tctcacgaac
     7081 gatgtcggct tgtggcgcgt acggctcgac cacgacgacc cgccccggcg ggtgcccggc
     7141 ggcgaccagg ccgcccacga tggcgctggc catgttgccg ccgccgacga aggcgagcag
     7201 cggcgcgggc gaggcggcgc aaggcccctg ggatggcgcg gtcgggggtg tcgggttcat
     7261 catggcgcga gtttaggttg cgacccgtcc gtaaccggca tgaaactgaa ccgcaggggt
     7321 catgcaaaag ttgcatgatg ctcggtgact aattcggcgg ggtccggcct acagtgcgca
     7381 ccactcccgg cagcggtgca tctccgatcg agaccgcctg cgcccaagga tcttgcgggt
     7441 cgccgctcac gaggccgacg cgacttccgc gaacctcaat cagtcacaag gaatccgtca
     7501 tgagcaatct gtcgtacgtc gcaccgaccg atgacagccc ctggggcacc tacctgtccc
     7561 aggtcgaccg cgtggtgccc tacctgggca acctgggccg ctgggccgag accctcaagc
     7621 gccccaagcg cgcgctgatc gtcgacgtgc ccatcgagct ggacaacggc acggtcgccc
     7681 acttcgaagg cttccgcgtg cagcacaacc tgtcgcgcgg cccgggcaag ggcggcgtgc
     7741 gctaccaccc cgacgtcacg ctggaagaag tgatggccct gtcggcctgg atgaccgtca
     7801 agtgcgcggc ggtgaacctg ccgttcggcg gcgccaaggg cggcatccgc gtcgatccca
     7861 agaagctgtc gcagccggaa ctcgagcgca tgacgcgccg ctacaccagc gagatcggcc
     7921 tgatcatcgg cccgcagaag gacatcccgg ccccggacgt gaacaccaac ggccagatca
     7981 tggcctggat gatggacacg tactcgatga acaccggctc cacgtccacc ggcgtggtca
     8041 cgggcaagcc gatccatctc ggcggctcgg ccggccgcgt caaggccacc ggccgcggcg
     8101 tgttcgtcac cggcagggaa gcggcgcgcc gcatcggcct ggacctcaac ggcgcgcgcg
     8161 tcgccgtcca gggcttcggc aacgtgggcg gctcggccgc cgagctcttc ggccaggctg
     8221 gcgcgaagat cgtcgcggcg caggaccaca ccggcaccat cttcaacgcc aacggctttg
     8281 acctgtccga gctgatgccg cacgtcgccc gcaccggcgg ggtggcgggc ttcaagggcg
     8341 ccgacacgat gaaggccgag gagttctggg acgtcgactg cgagatcatg atcccggccg
     8401 ccctcgaagg ccagatcagc gagtcgcgcg ccaagcgcat caaggccaag atggtcctcg
     8461 aaggcgccaa cggcccgacg acgccggccg ccgacgacat cctcgccgag cgcaagatcc
     8521 tggtcgtgcc cgacgtcatc tgcaacgccg gcggcgtgac ggtgtcgtac ttcgaatggg
     8581 tgcaggactt ctccagcttc ttctggagcg aggacgagat caacgtccgc ctggacaaga
     8641 tcatggtcga cgcgctcaag aagatctggg agacggccga ccgccacaag atcacgctgc
     8701 gcaccgccac gttcgcggtc gcctgcgagc ggatcctgat cgcgcgcgaa gagcgcggcc
     8761 tgtatccctg atccgacgcg tcgcgtcatc gaacgaaggg gccccgcggg gcccttttcc
     8821 attggcgcgc cggtgcgcgt caaccggccg ggcgcggcgc ggcgtccgtc gcatcgggtg
     8881 tgccgtcgtc cggcgccgca ggcggcgtgg gtggcggcgg cgacggctcg gcgctgacga
     8941 ccacccgctg caccctgtcc gacccgccgt cctgcgacgg ctcctccggg ctcttctcgt
     9001 gcggcttgac ggtggagcgg atcaccatga tgcgattgag cacgtcgaac cagaacggcg
     9061 cgcccagcat cacggcgagc gcggtcacca gccagcccag gcccatgcgc acgacggcgt
     9121 agaccgggtt gcggccgaac gtgcaggtct ccgacagcgc gcccgtgtcg tcggcgctcg
     9181 cgctgcagaa ctgcgtgggc cacagctgcg actcggcgcc gggcgtcgtc ggcgactccc
     9241 agccgatggg catcgtgacg gcgtcgagct cggcgcgtgc gagcgtcatc gcggcgtgct
     9301 cgtcgcccgc gcccgccgac gccggcggct tggccgcggc ggcgcggctc acgacgacgt
     9361 cgcggaacgt cttgtcgtgc gtcaggcggt gcaggatgtg cagcgcgtcg acgttgagca
     9421 ccacggccgt cagcaagccg atcgtgaaca gcagcgcctg cgtgcgccgc ttgtaccagc
     9481 ccgaggcccg atccatcgcg ccgtcgaacc actgctccac ggcctggcgc accttggcga
     9541 tgtcgccgtt ggcgtagtcg atgccggcga tcacggtgcg agccaggtgg tcgggcagct
     9601 gcgcggcctg cctgcgcagc agctcgatgg tcagggcccc gggcggcgcg gtgtcgggcg
     9661 cgtcgcccgg gccggcgggg ccgcgggcga cgaagtcgat gaatgccgtg gcgaagtgcg
     9721 cggtcgggat gtaggacggc aggttgcgac gtgcgcccag ccgcatgtgc ttgtcggcgc
     9781 cggtgcccat caccagccgg ctgctgcgca ggttccaaat cacgatcaat tcccttttca
     9841 aagcctgctg cagcagctcg ggctcttcct gc
//
LOCUS       DATCRO010000020        10703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_56453, whole genome shotgun sequence.
ACCESSION   DATCRO010000020 DATCRO010000000
VERSION     DATCRO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56453"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /locus_tag="VIN75_00495"
     CDS             complement(<1..1849)
                     /locus_tag="VIN75_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY8972663.1"
                     /translation="MSPFAKSDPTRAPHANVRLTATALAAASLFPLAAFAQTTAPAAT
                     ASAPAAAASATDATQTIYVTATRRREPAREVPMQVDRLSADDLDKGGAKNLSDYLGSQ
                     AGVDVKTSGGTGMGSVSIRGVTTGDQTIPTVGTYIDDVAYGSSSAFAAGSQTALDMSL
                     LDLNHIEVLRGPQGTLYGAGAMGGVVKYVTNEPDTSEFSGKVAIGASATKGGGAGNTV
                     NGVANIPLKTDVAALRLAAFHDHDGGWVDVLGPAAGKNANKGDTNGGRVALLVEPSAH
                     FHVRATALTQEIQRDNSDYTDIDPATGKPVAGWNQRTQVLREPHTVRTSMGSVDLEYD
                     FGGARLNSITSTQRSKMSLRYDLTGVYAPLITQFFGYTPDTVGEDEHTSVRKTTQEFR
                     LTSSAGGAVEWLAGLYWDRETGDLAQHVFDDGGGAGNIPVLADLDLPSRYEEIAGYGD
                     ITWNATSDLALTGGVRVAQNKQDFTQNASGPLVGGSLPTAHSKETPKTWLATARYALT
                     RTSNVYVRAASGYRPGGPNAVLNDPQTGLPTAPTTFQHDSLWSYEAGYKGDLLDNTLS
                     IETAVYDIRWNNIQQAYSVNGNGVLVNAGKAEIQGFELGATWHPSSNWLF"
     gene            2017..2718
                     /locus_tag="VIN75_00500"
     CDS             2017..2718
                     /locus_tag="VIN75_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8972664.1"
                     /translation="MKAPNPGPGGTIRAMRKRAGLTLAELSGRTGLAVSTLSKLEMGQ
                     VSLSYDKLMTISKGLGLDMAELLDSTPHPGGAPTHGGRRVVHRAGDGQHVETRSYSQS
                     YLATELLNKRMTPIYVELHARTLAEFKAEFGDLIRHPGEEFTYVLEGEVEFHTELYAP
                     LTLHAGDSIYFDSEMGHAYLKASDAPCRTITTCSPRGRDESMIDTFVNASAKHAEERA
                     QPRRKPVVKRAKAAK"
     gene            2862..3737
                     /locus_tag="VIN75_00505"
     CDS             2862..3737
                     /locus_tag="VIN75_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY8972665.1"
                     /translation="MDNATLTFNRLRPRLQGIAYRMLGSLAEAEEVVQDVWLRWHEAE
                     RDALDNPDGWLVTVTTRLAIDRYRAVKVQREHYVGQWLPEPHLTDSPRSPEQILERAD
                     ELSLAFLMVLERLSPEARAAFVMREVFDADYADVARIIDKSEAACRQLVSRAKTQLKE
                     ARPRYEVAPATHERLLRRFADAITGQDFSVIRDLLDDEAELIGDGGGQVPAFAHLAGG
                     QRIAQLFFASSLRHRGAMRVELARLNGQWGILRFIDGALESAQAIETDGERVVRIHTQ
                     RNPAKLARIAAALAR"
     gene            4298..4777
                     /locus_tag="VIN75_00510"
     CDS             4298..4777
                     /locus_tag="VIN75_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8972666.1"
                     /translation="MTARLDYSKQSPTLFKKFLDFSMAMHDSAVETPIRTLVEIRASQ
                     MNGCAFCLDMHVKQARIQGERDLRVHHVAIWRESPLFSPRERAALAWTEALTRIPEHG
                     VPDELYESVRKEFSDKEISDLTFEIMAINGWNRANAAFRNAPGAMDKAYGLDKAGLA"
     gene            complement(4781..5701)
                     /locus_tag="VIN75_00515"
     CDS             complement(4781..5701)
                     /locus_tag="VIN75_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972667.1"
                     /translation="MDLPDLNLLATLDVLLAEGNVARAAERLRLSPSAMSRQLARLRL
                     ATGDPLLVRAGRGLVPTPRAIELRERVGALVQDARAALGRAEALDLARLQRTFTLRTR
                     DGFVENFGAALVARVAAEAPGARLRFVPKPDKDNAPLRDGSVDLETGVVGQRTGPEMR
                     AQALLRDRFVGVVRAGHALARGRMSAARYAKAGHIVVSRRGDERTGIDDALDAMGLAR
                     RAVVIVDGFAPALALARDTDLVASVPERHTGRLRAGMHTFDLPVALPPFTVSLLWHPR
                     LHADAAHRWLRECVREVCAGEGNARRRRRA"
     gene            5814..7238
                     /locus_tag="VIN75_00520"
     CDS             5814..7238
                     /locus_tag="VIN75_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8972668.1"
                     /translation="MTATAHPAPATARQRWILASLCLTMLMPSLDTSIANAGLPVLAH
                     ALGAGFAHVQWIVLAYLLAITSLIVGAGRLGDLFGRRRLLVAGIGLFAAASLACGLAG
                     SLRALVAARALQGVGAAAMLALAVALVGETIPKERTGRAMGLLGTMSAIGTTLGPSLG
                     GALMTAFGWRSIFLVNVPLGLLNLWLARRWLPAPPRNAAAPRPRLDIAGTAVLGLTLA
                     AYALAMTARLPQFLAVAAVGAMAFVAVEVRAAAPLVRPAMFRDPRLASGLAASTLVAT
                     VIMTTLVVGPFYLSRALGLPPATVGLVLSAGPLASALAGAPAGRLVDRVGAPRVMLGG
                     LVAMLAGAIALALVPAAAGVPGYLGALLVMTLGYASFQPANNTALMADADAGRRGVVS
                     GLLNLSRNLGLITGASAMGAVFAAVAGDATTAGAAAVARGMHASFGVGAALLVVALGV
                     ALRGRALARGVASGAGASPGLSSG"
     gene            7287..7793
                     /locus_tag="VIN75_00525"
     CDS             7287..7793
                     /locus_tag="VIN75_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8972669.1"
                     /translation="MHIDFVAANQHDALISLMHEMCVFYSDAAPVSMDDVRSNLVDNL
                     LAPNSPVRLVVASDDGGDVVGFAAIAMFHSMVDPTPGRRGQMLLKELYVRQACQGRGI
                     GKALMAWVARHAIERGCARMDWHVSASNKPALAFYRALNAQHVAGRLNYRLGGEYLAR
                     LAGEDDSD"
     gene            7995..8363
                     /locus_tag="VIN75_00530"
     CDS             7995..8363
                     /locus_tag="VIN75_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8972670.1"
                     /translation="MKILVVDDHPDSVDSLSRLLEAHGHETARAYDGRQALDVGRQFE
                     PDVIVLDIDMPVLDGYGAARAIRLAPRARIPVLVALTALSGHETRRLAFEAGFDAHMT
                     KPARIDALLALIDKLVRERA"
     gene            complement(8565..9470)
                     /locus_tag="VIN75_00535"
     CDS             complement(8565..9470)
                     /locus_tag="VIN75_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972671.1"
                     /translation="MTTQLQDMDLFVRAVAAGSLSAAGRELGLSPAVASKRLARLEGR
                     LGARLLQRSSRRLSLTDNGALYLERCQAILAEVAEAEDLLGGDDAQVRGTLRVSATSG
                     LGRRWVGPVVAAFAARHPQVAVQLSLTDRVVDLVEAGLDCAVRVGPLADDRLIARKLA
                     DNRRVVCAAPAYLRTYGVPNTPADLARHACLVLATGNAMHADWRFRPPHGAATHVRVR
                     GRLASDNGQQVHDWMLAGHGLARRSFWDVADDLASGRLVEVLRDWSDEDAPISVIYAS
                     RRHLPRRTRLFIDALAAHFAQASTG"
     gene            9580..10302
                     /locus_tag="VIN75_00540"
     CDS             9580..10302
                     /locus_tag="VIN75_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8972672.1"
                     /translation="MTSSLANQTIAVFGASSGIGLAVARGAHALGARVIAIGRDRARL
                     AAAAGGLPLQLAEADIADEAQVAALLRDIGALDHVVVTAGSVVGPSPIASLDFDLARD
                     AIATKLQGSLLVAKHAAPRLRAGGSIGLTTGILGRRIAVNTVVKTVINAGLEAAVRQL
                     ARELAPLRVYGVSPGPVDTPNWGALGDDDRRARAAKFAATLPVGFMASADDTAQAYLF
                     AMQARALTGTVIDLDSGAMIAA"
     gene            10370..10597
                     /locus_tag="VIN75_00545"
     CDS             10370..10597
                     /locus_tag="VIN75_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972673.1"
                     /translation="MKRVVAGSHVHAETKTSFRYEATWHVEGQLLAWKAKVSLPGRRW
                     ALAGGTPEWTGGNEAKAVREDIARSIDGLEA"
BASE COUNT         1473 a         3849 c         3809 g         1572 t
ORIGIN      
        1 cgaacagcca gttcgagctc ggatgccagg tcgcacccag ctcgaagccc tggatctccg
       61 ccttgcccgc gttcaccagc acgccgttgc cgttgaccga gtaggcctgc tggatgttgt
      121 tccagcggat gtcgtagacg gccgtctcga tcgacagcgt gttgtccaac aggtcgccct
      181 tgtagccggc ctcgtagctc cagagcgagt cgtgctggaa cgtcgtgggc gcggtcggca
      241 ggccggtctg cgggtcgttc agcaccgcgt tcgggccgcc ggggcggtag ccgctggccg
      301 cgcggacgta gacgttgctg gtcctggtga gcgcgtagcg cgcggtcgcc agccaggtct
      361 tgggcgtctc cttggaatgc gccgtcggca gcgagccgcc caccagcggg ccgctggcgt
      421 tctgcgtgaa gtcctgcttg ttctgcgcga cgcgcacgcc gccggtcagc gcgagatcgg
      481 acgtcgcgtt ccacgtgatg tcgccatagc cggcgatctc ctcgtagcgg ctgggcaggt
      541 cgaggtcggc cagcaccggg atgttgccgg cgccaccgcc gtcgtcgaac acgtgttgtg
      601 ccaggtcgcc cgtctcgcga tcccagtaca ggccggccag ccattcgacc gcgccgcccg
      661 ccgacgaggt caggcggaac tcctgcgtcg tcttgcgcac ggaggtgtgc tcgtcctcgc
      721 ccacggtgtc gggcgtgtag ccgaagaact gcgtgatcag cggcgcgtag acgcccgtca
      781 ggtcgtagcg cagcgacatc ttcgagcgct gggtcgaggt gatcgagttc aggcgcgcgc
      841 cgccgaagtc gtactccagg tcgaccgagc ccatgctggt gcgcaccgtg tgcggctcgc
      901 gcagcacctg cgtgcgctgg ttccagccgg cgaccggctt gccggtggcc ggatcgatgt
      961 ccgtgtagtc cgagttgtcg cgctggatct cctgcgtcag cgcggtggcc cggacgtgga
     1021 agtgggcgct cggctcgacc agcagcgcca cgcggccgcc gttggtgtcg cccttgttcg
     1081 cgttcttgcc ggccgccggg ccgagcacgt cgacccagcc gccgtcgtgg tcgtggaacg
     1141 cggccaggcg cagcgcggcg acgtcggtct tgagcgggat gttggccacg ccgttgacgg
     1201 tgttgccggc gccgccgccc ttggtggcgc tggcgccgat ggcgaccttg ccggagaact
     1261 ccgaggtgtc gggctcgttg gtgacgtact tgaccacgcc gcccatcgcg ccggcgccat
     1321 acagcgtgcc ctgcgggccg cgcagcacct cgatgtggtt caggtcgagc agcgacatgt
     1381 ccagcgccgt ctgcgaacct gccgcgaacg cgctgctgga gccgtaggcc acgtcgtcga
     1441 tgtaggtgcc gacggtgggg atcgtctggt cgccggtggt cacgccgcgg atgctcaccg
     1501 agcccatgcc ggtgccgccg ctggtcttga cgtcgacgcc cgcctggctg ccgaggtagt
     1561 cgctcaggtt cttggcgccg cccttgtcca ggtcgtcggc cgacagccgg tcgacctgca
     1621 tcggcacctc gcgcgccggc tcgcggcggc gggtggccgt cacgtagatc gtctgcgtcg
     1681 cgtcggtggc gctggccgcg gcggcaggag cgctcgcggt ggcggcaggg gccgtcgtct
     1741 gggcgaaggc ggccagtggg aacagcgacg cggcggccag ggccgtcgct gtcaggcgga
     1801 cattcgcatg cggcgcccgg gtggggtctg acttggcgaa ggggctcatg gaaatcctcg
     1861 ttggagatga accggtcgtc ccgcctggat gtcgcgacgg ggccattttc aaatcctatc
     1921 cgcgattttc ttcttttcca agatttttcc gtttcggaaa ctttatcgtt ttcccgggga
     1981 tgtcttttcc gccagcccgg ctgatagagt cgcggcatga aagcgcccaa tcccggaccc
     2041 ggcggcacga tccgcgcgat gcgcaagcgc gccgggctca cgctggccga gctctcgggc
     2101 cgcacgggcc tggcggtgtc cacgctgtcc aagctggaga tgggccaggt ctcgctgtcc
     2161 tacgacaagc tcatgaccat cagcaagggc ttgggcctgg acatggccga actgctcgat
     2221 tcgacgccgc acccgggcgg cgcgcccacg catggcgggc gccgcgtggt gcaccgcgcc
     2281 ggcgacggcc agcacgtgga gacgcgcagc tacagccaga gctacctcgc gaccgagctc
     2341 ctgaacaagc gcatgacgcc catctacgtg gaactgcacg cgcgcacgct ggccgaattc
     2401 aaggcggagt tcggcgacct catccgccac cccggcgagg aattcaccta cgtgctggaa
     2461 ggcgaggtcg agttccacac cgagctctac gcgccgctga cgctgcacgc cggcgactcg
     2521 atctacttcg acagcgagat ggggcacgcc tacctgaagg cctccgacgc gccgtgccgc
     2581 accatcacca cctgctcgcc gcgcggccgc gacgagtcga tgatcgacac cttcgtcaac
     2641 gcgtcggcca agcacgccga ggagcgcgcg cagccgcggc gcaagccggt ggtcaagcgc
     2701 gcgaaggcgg cgaagtagcc cggctcgcgc tcttgcggtg ccacttgcgg tgccaggtct
     2761 tgcctgtcaa ggcgaaaggc aagacctgac accggagatt tcgccattcc ttgcgcgcac
     2821 ggccgtcaca agtcgcccgc ctcgaacgtc atggtggaac catggacaac gccaccctca
     2881 ccttcaaccg cctgcggccc cggctgcagg gcatcgccta ccgcatgctg ggctcgctcg
     2941 ccgaggccga ggaggtcgtg caggacgtct ggctgcgctg gcacgaggcc gagcgcgacg
     3001 cgctcgacaa ccccgacggc tggctggtga ccgtcaccac ccggctggcg atcgaccgct
     3061 accgcgccgt caaggtgcag cgcgagcact acgtcggcca gtggctgccc gagccgcacc
     3121 tgaccgactc gccgcgctcg cccgagcaga tcctggagcg cgccgacgag ctgtcgctcg
     3181 ccttcctgat ggtcctcgag cgcctgtcgc ccgaggcgcg cgccgcgttc gtcatgcgcg
     3241 aggtgttcga cgccgactat gccgacgtgg cgcgcatcat cgacaagtcc gaggcggcct
     3301 gccggcagct ggtcagccgt gcgaagacgc agctcaagga ggcgcgcccg cgctacgagg
     3361 tcgcgcccgc gacgcacgag cgcctgctgc ggcgcttcgc cgacgcgatc accgggcagg
     3421 atttctcggt catccgcgac ctgctcgacg acgaggccga gctcatcggc gacggcggcg
     3481 gccaggtgcc ggccttcgcc cacctggcgg gcggccagcg catcgcgcag ctgttcttcg
     3541 cctccagcct gcgccaccgt ggcgcgatgc gcgtggagct cgcgcggctc aacggccaat
     3601 gggggatcct gcgcttcatc gacggcgcgc tggagtccgc ccaggcgatc gagaccgacg
     3661 gcgaacgcgt ggtgcgcatc cacacgcagc gcaacccggc caagctggcg cgcatcgcgg
     3721 ccgcgctggc ccgctgagca aagagcgacg gggggcagcc ggaggggcct ggcccctcct
     3781 cgagatcgcg ccacggcttt cccgccagcc gatgcgcagc tgcgatctcg aggccagggc
     3841 ctgacccccg atcatcggcc ggggttcggg gtcaggcctt cgcctcgtgc cgcctgaagc
     3901 agcctcgcgc atggtcgcat cccggcggcg tcatgaggag aggccagacc cctcgggctg
     3961 cctcgctctc acaaaattcc atacgggggc cagccggagg ggactggccc ctgctcgaga
     4021 tcgcgccacg gcgttcccgc cagccgatgc gcagctgcga tctcgaggcc agggcctgac
     4081 ccccgatcat cggccggggt tcggggtcag gccttcgcct catgccgccc gaagcagcct
     4141 cgcgcatggt cgcatcccgg cggcgtcatg aggagaggcc agacccctcg ggctgccgcg
     4201 ctctcacaaa aaaattccat gcgagtgtca caaccgcctt cgccgcttcg tcatggaggt
     4261 tgtggacacc ccactcaccc caacccagga gcacgccatg accgcccgcc tcgactattc
     4321 caagcaatcg cccaccctgt tcaagaagtt cctcgacttc agcatggcca tgcacgacag
     4381 cgcggtcgag acgccgatcc gcacgctcgt cgagattcgc gcctcgcaga tgaacggctg
     4441 cgccttctgc ctggacatgc acgtcaagca ggccaggatc cagggcgagc gcgacctgcg
     4501 cgtgcaccac gtcgccatct ggcgcgagtc gccgctgttc tcgccgcgcg agcgcgccgc
     4561 actcgcctgg accgaggcgc tgacgcgcat ccccgagcac ggcgtgcccg acgagctcta
     4621 cgagagcgtg cgcaaggagt tttccgacaa ggagatctcc gacctcacgt tcgagatcat
     4681 ggccatcaat ggctggaacc gcgccaacgc cgccttccgc aacgcgccgg gcgcgatgga
     4741 caaggcctac ggcctggaca aggccggcct ggcctgagca tcacgcccgg cggcggcggc
     4801 gcgcgttgcc ctcgccggcg cacacctcgc gcacgcactc gcgcagccag cggtgcgccg
     4861 catcggcgtg caggcgcggg tgccacagca gcgagaccgt gaacggcggc agcgcgaccg
     4921 gcaggtcgaa ggtgtgcatg cccgcgcgca gccggccggt gtgccgctcg gggacgctcg
     4981 cgacgagatc ggtgtcgcgc gcgagcgcca gcgcgggcgc gaagccgtcg acgatgacga
     5041 cggcgcgccg agccaggccc atcgcgtcga gcgcatcgtc gatgccggtg cgctcgtcgc
     5101 cgcggcgcga gacgacgatg tgcccggcct tcgcgtagcg cgccgcgctc atgcgcccgc
     5161 gcgcgagcgc gtggcccgcg cgcacgacgc cgacgaagcg gtcgcgcagc agcgcctgcg
     5221 cgcgcatctc gggaccggtg cgctggccga cgacgccggt ctccaggtcg acgctgccgt
     5281 cgcgcagcgg cgcgttgtcc ttgtccggct tgggcacgaa gcgcagccgc gcgcccggcg
     5341 cctctgcggc cacgcgggcg accagcgcgg cgccgaagtt ctcgacgaag ccgtcgcgcg
     5401 tgcgcagcgt gaacgtgcgc tgcagccgtg cgaggtccag cgcctcggcc cggccgagcg
     5461 cggcgcgggc gtcctgcacc agcgcgccga cgcgttcgcg cagttcgatg gcgcgcggcg
     5521 tcggcaccag gccgcggccg gcacgcacca gcagcggatc gccggtggcc aggcgcaggc
     5581 gcgcgagctg gcgactcatc gccgacgggc tgagccgcag gcgctcggcg gcgcgggcga
     5641 cgttgccctc ggcgagcagc acgtccagcg tcgcgagcag gttcaggtcg ggcaggtcca
     5701 tcgccgcatc ctgccatgac atggcgttgc gtgcactgtt cgaatgcaat cagtgcgtct
     5761 tccgccatgg ctccgatcgt cctacgctgg ccttccggac acgaaggacg acgatgaccg
     5821 ccaccgccca ccccgctccc gccaccgcgc gccagcgctg gatcctcgcc agcctttgcc
     5881 tgacgatgct gatgccctcg ctggacacca gcatcgccaa cgccgggctg ccggtgctcg
     5941 cgcacgcgct gggcgccggc ttcgcgcacg tgcagtggat cgtgctggcg tacctgctgg
     6001 ccatcacgtc gctgatcgtc ggcgccggcc ggctcggcga cctgttcggg cggcgccgcc
     6061 tgctggtcgc gggcatcggg ctgttcgccg cggcgtcgct cgcatgcggg ctggccggct
     6121 cgctgcgcgc gctggtcgcg gcgcgcgcgt tgcagggcgt gggcgcggcg gcgatgctcg
     6181 cgctggcggt ggcgctggtc ggcgagacga tccccaagga gcgcacgggc cgcgcgatgg
     6241 gtctgctcgg gacgatgtcg gcgatcggca cgacgctggg accgtcgctg ggcggcgcgc
     6301 tgatgacggc cttcggctgg cgcagcatct tcctggtcaa cgtgccgctc ggcctgctga
     6361 acctgtggct ggcgcggcgc tggctgcccg cgccgccgcg caatgccgcg gccccgcggc
     6421 cgcgcttgga catcgccggc accgccgtcc tcggcctcac cctggcggcc tacgcgctcg
     6481 cgatgacggc gcgcctgccg cagttcctgg ccgtggccgc cgtgggggcg atggccttcg
     6541 tcgcggtcga ggtgcgggcg gcggcgccgc tggtgcggcc ggcgatgttc cgcgatccgc
     6601 gcctggccag cggcctggcc gccagcacgc tggtggccac ggtgatcatg acgacactgg
     6661 tcgtcgggcc gttctatctg tcgcgcgcgc tgggcctgcc gccggccacc gtgggcctgg
     6721 tgctgtcggc cgggccgctg gcgtcggcgc tcgcgggagc gcccgcgggg cgcctcgtcg
     6781 accgggtggg cgcgccccgg gtgatgctgg gcggcctggt ggcgatgctg gccggcgcca
     6841 tcgcgctcgc gctcgtgccg gcggcggccg gcgtaccggg ctacctcggc gcgctgctcg
     6901 tgatgaccct cggctatgcg agcttccagc cggcgaacaa cacggcgctg atggcggacg
     6961 ccgatgcggg acggcgcggc gtcgtgtccg gcctgctgaa cctgtcgcgc aacctcgggc
     7021 tgatcaccgg cgcgtcggcg atgggcgcgg tgttcgccgc ggtggccggg gacgccacga
     7081 ccgccggcgc cgccgcggtg gcgcgcggga tgcatgccag cttcggcgtg ggcgcggcgc
     7141 tgctcgtcgt cgcgctgggc gtggcgttgc ggggtcgcgc cctggcccgt ggcgtcgcgt
     7201 caggtgcggg ggcgagtccc gggttaagct cgggttgaga aaggcggcca gccggtcgcc
     7261 tgcgcaccgc tgcccggggg aggcgcatgc acatcgactt cgtggccgcg aaccagcacg
     7321 atgccctgat ttccctgatg cacgagatgt gcgtcttcta cagcgacgcc gcgcccgtct
     7381 ccatggacga cgtgcgctcc aatctcgtgg acaacctgct ggcgccgaac tcgcccgtgc
     7441 gcctggtggt cgcgtccgac gacggcggcg acgtggtggg cttcgcggcg atcgcgatgt
     7501 tccactcgat ggtcgacccg acacccggcc ggcgcggcca gatgctgctc aaggaactct
     7561 acgtgcgcca ggcctgccag ggacgcggca tcggcaaggc cttgatggcc tgggtcgcgc
     7621 gccatgcgat cgagcgcggc tgcgcgcgca tggactggca tgtcagcgcc agcaacaagc
     7681 ccgcgctggc gttctaccgt gcgctgaacg cgcagcacgt cgcgggccgg ctgaactacc
     7741 gcctcggcgg cgagtacctg gcgcggctgg cgggcgagga cgactcggac tgaagcgggg
     7801 ccggcgtcgg cggccgcgca aacgtttccg tcgtcgcagg tttcaacgcg tcacactttc
     7861 cccgggtcct cgtgcggtag ggcggccaca acgccgggtg aacccggcca tggccgcgcg
     7921 gcgatcctcg ctaggatggt ccgcaaacac caagaccgtc acgggaggct tccttcgaga
     7981 gaggcccggt tccaatgaag atcctcgtgg tggacgacca ccccgacagc gtcgactcgc
     8041 tgagccggct gctggaagcg catgggcacg agaccgcccg cgcctacgac ggccgccagg
     8101 cgctcgacgt cggccgccag ttcgagccgg acgtcatcgt gctggacatc gacatgcccg
     8161 tgctcgatgg ctacggcgcc gcccgcgcca tccgcctcgc gccccgcgcg cgcatccccg
     8221 tgctggtcgc gctcaccgcg ctcagcggcc atgagacccg ccgcctcgcg ttcgaggccg
     8281 gcttcgacgc gcacatgacc aagcccgcac gcatcgatgc gttgctggcg ctgatcgaca
     8341 agctggtgcg ggagcgggcc tagtgtcgtg attgggaagt tcgttgaatg ggtcgcgagc
     8401 gcagaggaaa cgttgccgaa tcctctcgca aacgtctgtg gactaggcgg cgcgcggagc
     8461 gaggctcaat gcctcgcgag cacggcaacg acggccacgg gcgttttcga ggggattctt
     8521 caacgaactt ctcaatcacg acactagcgc gccggcgacg ccgctcagcc cgttgacgcc
     8581 tgcgcgaaat gcgccgccag cgcatcgatg aacagccgtg tgcgccgcgg caggtggcgg
     8641 cgggacgcgt agatcaccga gatcggcgcg tcctcgtcgc tccagtcgcg caacacctcc
     8701 acgaggcgac cgctcgcgag gtcatcggcg acatcccaga acgagcggcg cgcgaggccg
     8761 tggccggcga gcatccagtc gtgcacctgc tggccgttgt cggacgcgag ccggccgcgc
     8821 acgcgcacgt gcgtggccgc gccgtgcggc gggcggaagc gccagtcggc gtgcatcgcg
     8881 ttgccggtgg ccagcaccag gcacgcgtgg cgcgcgagat cggccggagt gttcggcacg
     8941 ccatacgtcc tgaggtatgc gggcgcggcg cacaccacgc ggcggttgtc ggccagcttg
     9001 cgcgcgatca ggcgatcgtc ggccagcggc ccgacgcgca ccgcgcagtc caggccagcc
     9061 tcgaccaggt cgacgacgcg gtcggtcagc gacagttgca ccgcgacctg cggatgccgc
     9121 gccgcgaacg ccgcgacgac gggcccgacc cagcgccggc ccagtcccga ggtggccgac
     9181 acccgcagcg tgccgcgcac ctgcgcgtcg tcgccgccca gcaggtcctc ggcctcggcc
     9241 acctcggcca ggatcgcctg gcagcgctcg aggtacagcg cgccgttgtc ggtcaggctc
     9301 aggcggcgcg agctgcgctg cagcagccgc gcgcccagcc gcccctccag ccgtgcgagg
     9361 cgcttgctcg ccacggccgg cgaaaggccg agttcgcgcc ccgcggccga caggctcccc
     9421 gccgcgacgg cgcgcacgaa cagatccatg tcctgcaact gggtcgtcat gtctggactt
     9481 caactttctg gaaaagctgg ttatcgattt tgacggattc cgttccaccg tggaacagat
     9541 caaagtgctg ccatcgcaac ccgtctcacg gagaacacca tgacctcgtc cctcgccaac
     9601 cagaccatcg ccgtgttcgg cgccagctcc ggcatcggcc tcgccgtcgc gcgcggcgcg
     9661 cacgcgctcg gcgcgcgcgt gatcgcgatc gggcgcgacc gcgcgcgcct ggccgcggcc
     9721 gccggcggcc tgccgctcca gctcgccgag gccgacatcg ccgacgaggc gcaggtggcg
     9781 gcgctgctgc gcgacatcgg cgcgctcgac cacgtggtcg tcacggcagg cagcgtggtc
     9841 ggcccgtcgc cgatcgcgtc gctcgacttc gacctcgcgc gcgacgccat cgccaccaag
     9901 ctgcagggca gcctgctcgt ggccaagcac gccgcgccac gcctgcgcgc gggcggctcc
     9961 atcggcctga ccaccggcat cctgggccgc aggatcgcgg tcaacaccgt cgtcaagacc
    10021 gtcatcaacg ccggcctcga ggccgccgtg cggcagctcg cgcgcgagct cgcgccgctg
    10081 cgcgtctacg gcgtcagccc gggccctgtg gacacgccca actggggcgc cctcggcgac
    10141 gacgaccgcc gggcccgcgc cgcgaagttc gcggccacgc tgccggtggg cttcatggcc
    10201 agcgccgacg acaccgcgca ggcctacctg ttcgcgatgc aggcgcgtgc gctcacgggc
    10261 accgtcatcg acctcgacag tggtgcgatg atcgccgcct gaagcgcggt attgacgcgg
    10321 ccatgccggg ccgcgaggat ggccggctca tccaggggag gccagggact tgaagcgtgt
    10381 cgtcgccggc tcgcacgtgc acgcggagac caagacgtcg ttccgctatg aagccacctg
    10441 gcacgtggaa gggcagctgc tcgcctggaa ggccaaggtc tcgctgcccg gccggcgctg
    10501 ggcgctggcc ggcggcacgc ccgagtggac cggcggcaac gaggccaagg ccgtgcgcga
    10561 ggacatcgcg cgcagcatcg acggcctcga ggcctgacgc gcagggtgcg acacgggggg
    10621 tcaggtcttg aaagttgcgc gatcagccat gcgaccatgg ggtcaggtct tgaaagttgc
    10681 gcggcaaggt ttcggcaatg gat
//
LOCUS       DATCRO010000021         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_315247, whole genome shotgun sequence.
ACCESSION   DATCRO010000021 DATCRO010000000
VERSION     DATCRO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315247"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1294
                     /locus_tag="VIN75_00550"
     CDS             491..1294
                     /locus_tag="VIN75_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8972674.1"
                     /translation="MDLQLKGKRALVTGGSKGIGKAVARALLLEGADVALLARHEQTL
                     LDAVSELALQTGGEVIAVVADTTSDEQVRQAVATAARQLGGSIDILVNAAAEVAGYAN
                     PPRLEEIAAEAFHREMDIKVMGYVRCAREVVPGMRAAGWGRIVNVSGLAARQSGSAVG
                     SMRNIAVAALTKNMADELGPSGIQATVVHPGTTRTERTAALVAQKAAATGRSVADVER
                     EMAQGNSIHHLVDAREVADVVAFLCSPRSRAINGDAIAVGGGAPRAIHY"
     gene            1310..2452
                     /locus_tag="VIN75_00555"
     CDS             1310..2452
                     /locus_tag="VIN75_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8972675.1"
                     /translation="MKLKKFDTLFARIFLLLLVAFSVSSWLAFEVFRATAPQPPWRDT
                     PPIRQSMHPLPGSTAPPPALPPGKGWPDRPHPRQPPWLSIGLDAVIKIITVALAAWVA
                     ARALSQPMRRLADAARRLGQSVSPTAGMGIAVAPPQLDEERGTREVRETARVFNDLSR
                     QLKSDFDTQRLMLATVSHDLRTPLTRLRMRLSELEAHPAARSAIDDVRQMDELIEGLL
                     ALFRPPAPSAPGGAGRIDAFALVQAIVDDLPPGGPEVALSGTSATTPCPPAAFRRMVE
                     NLIENAIRHGGGQGAVEVEVTTGPANVCVTVMDRGPGLSAEQLASAGTPFLRWSGNES
                     DSGRRAGLGLGLYITRALAERHGGRLELAARQGGGLCATLHLPLSS"
BASE COUNT          360 a          967 c          937 g          325 t
ORIGIN      
        1 agcgggctgt cggcggcggc cggcagcgca tggccgggcg tcttctgcag gtccgccagc
       61 gacgcgcagc cctgcagcgc cagcgccagc atggccaggg ccgccgcgcg cgcccacgcc
      121 ctcacgcgcg gccccgcgcg cgggaggcgg acgaacgacg gcaggcaagg cacatggcga
      181 tcggcgaagt cgacagcaac acggggatca cgttgcccgg caatgatagg cggccgggct
      241 cgcgcccgcg tcgccctgcc cgccgattgc ccttcgtcgc cgaaaggctg tcatcctgcg
      301 cgcagtcgca ggatccacct tgcctggcca cgatgccgcc cggtacgccg acgtggatcc
      361 tgcgacttcg cgcaggatga cggggttggc atgcctgcag gacgacgggg ttggcgtgcc
      421 tgcgcgatgg cgacggactg cacgatccgc ccgcgtcaga atcacctatc cacacccacg
      481 aaagttcgac atggatctgc agctcaaagg caagcgcgcc ctggtcaccg gcggcagcaa
      541 ggggatcggc aaggccgtcg cgcgggcgct gctgctcgag ggcgccgacg tcgcgctgct
      601 cgcgcgccac gagcagacgc tgctggacgc cgtctccgaa ctggcgttgc agacgggcgg
      661 cgaggtcatc gccgtcgtcg ccgacaccac cagcgacgag caggtgcgcc aggccgtggc
      721 cacggccgcc aggcaactgg gcggctcgat cgacatcctc gtgaacgccg ccgccgaggt
      781 ggccggctac gcgaaccctc ccaggctcga ggagatcgcc gccgaggcct tccaccgcga
      841 gatggacatc aaggtgatgg gctacgtgcg ctgcgcgcgg gaggtcgtgc cgggcatgcg
      901 cgccgccggc tggggccgca tcgtcaacgt cagcggcctg gccgcgcgcc agagcggcag
      961 cgcggtgggc tcgatgcgca acatcgcggt ggccgcgctc accaagaaca tggccgacga
     1021 gctgggtccg tccggcatcc aggcgaccgt ggtgcacccg ggcacgaccc gcaccgagcg
     1081 cacggcggcg ctggtcgcgc agaaggccgc cgcgacggga cgcagcgtcg cggacgtcga
     1141 gcgcgagatg gcgcagggca acagcatcca ccatctcgtc gacgcccgcg aggtggccga
     1201 cgtcgtcgcc ttcctgtgct cgccgcgctc gcgcgcgatc aacggcgacg ccatcgccgt
     1261 gggcggcggc gcgccgcgcg ccatccacta ttgaccccgc tggccggccg tgaagctcaa
     1321 gaagttcgac acgctgttcg cacgcatctt cctgctgctg ctcgtggcgt tctcggtcag
     1381 ctcgtggctg gcgttcgagg tgttccgcgc gaccgcgccg cagccgccgt ggcgcgacac
     1441 gccgccgatc cgccagtcga tgcatcccct gccgggcagc accgcgccgc cgccggcgct
     1501 gccgcccggc aagggctggc ccgaccggcc gcacccgcgc cagccgccgt ggctgtcgat
     1561 cggcctggac gccgtcatca agatcatcac cgtggcgctg gccgcctggg tcgcggcgcg
     1621 cgcgctgtcg cagccgatgc ggcgcctcgc cgacgccgcg cggcggctcg ggcagtcggt
     1681 gtcgccgacc gcgggcatgg gcatcgccgt cgcgccgccg cagctcgacg aggaacgcgg
     1741 cacgcgcgag gtgcgcgaga cggcgcgcgt gttcaacgac ctgtcacgcc agctcaagag
     1801 cgacttcgac acccagcgcc tgatgctggc caccgtctcg cacgacctgc gcacgccttt
     1861 gacgcgcctg cgcatgcggc tgtccgaact cgaggcgcac ccggcggcgc gcagcgcgat
     1921 cgacgacgtg cggcagatgg acgagctgat cgagggcctg ctcgcgctgt tccgcccgcc
     1981 cgcgcccagc gcgcccggcg gcgcgggacg catcgacgcc ttcgcgctgg tgcaggccat
     2041 cgtcgacgac ctgccgcccg gcggccccga ggtcgcgctc tccggcacgt cggcgacgac
     2101 gccgtgcccg ccggccgcgt tccgccgcat ggtggagaac ctgatcgaga acgcgatccg
     2161 ccatggcggc ggccagggcg cggtggaggt ggaggtgacg accggcccgg ccaacgtctg
     2221 cgtgaccgtg atggaccgcg ggccggggct cagcgccgag cagctcgcca gcgcgggcac
     2281 gccgttcctg cgctggtccg gcaacgaatc ggacagcggc cgccgcgccg gcctgggcct
     2341 cgggctctac atcacgcgcg cgctcgccga acgccacggc ggacggctgg agctggccgc
     2401 acgccagggg ggcgggctct gcgcgacgct gcacctgccg ctgtcgagct aggagtctgc
     2461 gcggcagaag gcgcctgctg cgaacggcga taagcggggc cttttcagcg gcggctcctc
     2521 tcgcgtagcg cccactacga gttcgtcgcc gccgctgaaa agtccatgct tctcgccgct
     2581 ctcgcgacg
//
LOCUS       DATCRO010000022         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_495264, whole genome shotgun sequence.
ACCESSION   DATCRO010000022 DATCRO010000000
VERSION     DATCRO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495264"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VIN75_00560"
     CDS             complement(<1..158)
                     /locus_tag="VIN75_00560"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345974.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate,
                     phenylalanine sensitive; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8972676.1"
                     /translation="MSNAKPTPEARHTLSLSERTSDTDDQRIRDVTPLPPPEHLIRFF
                     PIAGTPVET"
     gene            complement(487..1950)
                     /gene="tldD"
                     /locus_tag="VIN75_00565"
     CDS             complement(487..1950)
                     /gene="tldD"
                     /locus_tag="VIN75_00565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY8972677.1"
                     /translation="MITREPTIERLAIAEGLLLAPFGLTETNLAATLAAMGEHRIDDA
                     DLYFQTTRHEGWSLEEGIVKSGSFSIDQGVGVRAVAGEKTAFAYSDDISASSLMDAAR
                     TVRAIAAAGQSKRVKLQGKTKISGSRLLYGTGDPIATLDSTQKVALLERIEKMARARD
                     PRIVQVMAGLAAEHDVVLIARADGTRAADVRPLVRLSITVIAEQNGRRETGSGGGGGR
                     FGLGYFTEQILSRYVDQAVNAALTNLESRPAPAGQMTVVLGPGWPGVLLHEAVGHGLE
                     GDFNRKGSSTFAGRIGKRVAAKGVTVLDDGTIADRRGSLNVDDEGNPSQRNVLIEDGI
                     LRGYIQDSLNARLMGVKPTGNGRRESYAHTPMPRMTNTYMLGGDTPREEIVASIKRGL
                     YATNFGGGQVDITSGKFVFSASEAYWVENGKIQYPVKGATLIGNGPDALTRISMIGND
                     MELDSGVGVCGKEGQSVPVGVGQPTLRIDGLVVGGTA"
     gene            2122..>2463
                     /locus_tag="VIN75_00570"
     CDS             2122..>2463
                     /locus_tag="VIN75_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972678.1"
                     /translation="MSNRHLLVIAGMALASSAAFAQGTYDQPRYDDQQQYDQQRHDAN
                     GYSRAAWREQQRSAEQEQRIREGQANGSITPREAAYLLEQQRNIRHVEWRANRDGVLT
                     PDERRQIRYMQD"
BASE COUNT          362 a          833 c          858 g          410 t
ORIGIN      
        1 gtctccaccg gcgtgcccgc gatcgggaag aagcggatca ggtgctcggg cgggggcagc
       61 ggggtgacgt cgcggatgcg ctggtcgtcg gtgtcgctgg tgcgctcgct cagcgacagc
      121 gtgtggcgcg cttcgggcgt cggcttggca ttgctcatca tctgctccgg gtcttctcgt
      181 gagtctggcg attgcagggt gccggcaccg gcgaaaaaaa accgccgggt gggccggcgg
      241 tctttcgaag gtccttcgct gcttacttgc gcgcgatcgc ttctcgagcc acctgacggg
      301 gcgagaacca aaagtacgaa aagaagaagc gcgagacgtt catggagatt cgaatgtagc
      361 acggcgggcg agccacgtgg cttcgcgcgg tggcaagggg cgcttcagcc ggggtggatc
      421 ctgcgacttt gcgcaggatg acaacaccct ctgagtcatc ctgcgcgaag tcgcaggatc
      481 cacgcctcat gcggtgccgc ccaccaccag gccgtcgatg cgcagcgtgg gctggcccac
      541 gcccaccggc acgctctggc cttccttgcc gcagacgccc acgccggagt cgagctccat
      601 gtcgttgccg atcatcgaga tgcgcgtcag cgcgtccggg ccgttgccga tcagcgtggc
      661 gcccttgacc gggtactgga tcttgccgtt ctcgacccag taggcctcgc tcgccgagaa
      721 cacgaacttg ccgctggtga tgtcgacctg gccgccgccg aagttggtgg cgtacaggcc
      781 gcgcttgatc gacgcgacga tctcctcgcg cggcgtgtcg ccgcccagca tgtaggtgtt
      841 ggtcatgcgc ggcatcggcg tgtgcgcgta gctttcgcgg cggccgttgc cagtgggctt
      901 gacgcccatc aggcgcgcgt tgagcgagtc ctggatgtag ccgcgcagga tgccgtcctc
      961 gatcagcacg ttgcgctggc tcgggttgcc ctcgtcgtcg acgttgagcg agccgcggcg
     1021 gtcggcgatg gtgccgtcgt ccagcaccgt gacgcccttg gccgccacgc gcttgccgat
     1081 gcggccggcg aacgtgctgg agcccttgcg gttgaagtcg ccttcgaggc catggcccac
     1141 cgcctcgtgc agcagcacgc cgggccagcc cgggcccagc accacggtca tctggccggc
     1201 cggcgccggc cgcgactcga ggttcgtcag cgccgcgttg acggcctggt cgacgtacct
     1261 ggacaggatc tgctcggtga agtagccgag gccgaagcgg ccgccgccgc cgcccgagcc
     1321 cgtctcgcgg cgcccgttct gttcggcgat gacggtgatc gacaggcgca ccagcggccg
     1381 cacgtcggcg gcgcgcgtgc cgtcggcgcg cgcgatcagc acgacgtcat gctcggcggc
     1441 caggccggcc atcacctgca cgatgcgcgg gtcgcgggcg cgcgccatct tctcgatgcg
     1501 ctccagcagc gccaccttct gcgtggagtc gagcgtggcg atcgggtcgc ccgtgccgta
     1561 cagcaggcgg ctgccggaga tcttggtctt gccctgcagc ttgacgcgct tgctctggcc
     1621 cgcggcggcg atggcgcgca cggtgcgcgc ggcgtccatc agcgagctcg cggagatgtc
     1681 gtcggagtac gcgaacgccg tcttctcgcc ggcgacggcg cgcacgccca cgccctggtc
     1741 gatcgagaag ctgccgctct tgacgatgcc ttcctccagg ctccagccct cgtggcgggt
     1801 ggtctggaaa tagaggtcgg cgtcgtcgat gcggtgttcg cccatcgcgg ccagggtcgc
     1861 cgcgaggttc gtctcggtca ggccgaacgg cgccagcagc aggccctcgg caatggccag
     1921 tcgttcgatc gtcggttcgc gtgtgatcac ggcgggctcc tggtttgctg gttcagggac
     1981 aagccggcac gttccggcga atccagggaa aggtggctcg attgtgcagg ttgtacgccg
     2041 gctggcggcg cctgcgcgag cgtcgaagga aaagttccat cagcggtcga gctgccagcc
     2101 ctcttgaata aggagtctga catgtccaac cgccatctgc tcgtcatcgc cggcatggcc
     2161 ctcgcctcga gcgcggcctt cgcccagggc acctacgacc agccgcgcta cgacgatcag
     2221 cagcagtacg accagcagcg ccacgacgcc aacggctatt cccgcgccgc ctggcgcgag
     2281 cagcagcgca gcgccgagca ggaacagcgc atccgcgaag gccaggccaa cggcagcatc
     2341 acaccccgtg aagccgctta cctgctcgag cagcagcgca acatccgcca tgtggaatgg
     2401 cgggccaatc gtgacggcgt gctgacgccc gacgagcgcc ggcagatccg ctacatgcag
     2461 gac
//
LOCUS       DATCRO010000023         7675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_619054, whole genome shotgun sequence.
ACCESSION   DATCRO010000023 DATCRO010000000
VERSION     DATCRO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619054"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIN75_00575"
     CDS             complement(<1..214)
                     /locus_tag="VIN75_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8972679.1"
                     /translation="MNSITAPSQGQPGQVDAVADAVYRKVAWRLIPLLFLCYIAAYLD
                     RVNVGFAKLQMQQALQFSDSVYGFGAG"
     gene            complement(433..1512)
                     /locus_tag="VIN75_00580"
     CDS             complement(433..1512)
                     /locus_tag="VIN75_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8972680.1"
                     /translation="MTRHQSRVLDALAAAAALACAGHAFAQSNETYNPNAYILLNGSA
                     VKPDSKYPEDKTGWGGGLKVGIPLNPSWDLQVGGNYAASTDHGANFHQTLAGVDALYL
                     FTPGGFRPFVLFGAGAARDHFGDGVNGTFKKTAPYVDAGLGFQYKFTPTLGMQADIRE
                     VGTFYSSRVQPFDMKHTANTYANVGLTWAFGGPPAPEPVPVATTTRTVETTTVTTPPP
                     PPPPPAPLPPPPPVMKKYTLSASELFGFDSAKLGPNQPKLDEVAATMNANADIASVTI
                     VGYTDRLGSKQYNQQLSEKRANAVKAYLEGKGVAGNRLTAVGKGEADPVVECKDEKKK
                     AALIKCLEPNRRVEIEPVTFTKQVN"
     gene            1919..2494
                     /locus_tag="VIN75_00585"
     CDS             1919..2494
                     /locus_tag="VIN75_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972681.1"
                     /translation="MRLPHSCAALAAATFLCSAPARGAEIVLEQSAVQKLVVESLFKD
                     NGRFYIQRGACSAYLDNAAVALTGGRVVIRSHLSARVGMDLGDSCAGVDLASWATVSG
                     VPSAQGTTVRLSDIRIEDVGDANMRIVLNSGLVPTLPAAFELDVLKAVRSMLQGADSP
                     LQVDVQALRIDSVRVADNRLSVRFDFRVAGR"
     gene            complement(2522..3781)
                     /locus_tag="VIN75_00590"
     CDS             complement(2522..3781)
                     /locus_tag="VIN75_00590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8972682.1"
                     /translation="MTTRSCTARLLPPALAAAILLAAAGSATAATHAKPKAKPHAAAR
                     KAAESTEPAMTTDANGRSVPVLASKAWVLMDFDSGQILASSNADEPLPPASLTKMMTS
                     YLVEQGLKSGKLKPTEMVTMSLSAWCKGGKEGDSCMFVPLNSQASLMDMLRGVVIDSG
                     NDASKALAEHIGGSESGFVAMMNAEAAKLGMTHTHFENSAGLPDPNHKASARDLAVLA
                     QAIIRNSAEYYPIYAEHEFTFNGIKQGNRNALLYTDPTVDGLKTGHTEEAGYCLTASA
                     KRNGLRLISVIMNADSAQARADQTRVLFNWGYANFEQATPVKADATLATAKVTYGMVP
                     TVAVGVPKAWTLLVPKGQAGAVQTAVTLNPTLEAPIAKGAVVGKVVATANGKPLGETP
                     VVALAAVERAGFFQRLGQRITGLFSRQ"
     gene            complement(3951..5558)
                     /locus_tag="VIN75_00595"
     CDS             complement(3951..5558)
                     /locus_tag="VIN75_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8972683.1"
                     /translation="MTETSRRAFLGAALAAPAILTGCAKPEDWRTRVEGRWVGDNSAL
                     GHRVRDRARDAAAHPPAGARERRCDVLIVGSGIAGLSAARTLQRAGITDFAVLELEDE
                     PGGNSRGHAMQGLRCPIGAHYLPLPGPEAGDIQDWLVGIGLAKSTAAGLKWDERHLCH
                     SPQERLYINGRWQDGMLPLVPRDSATQAQYRLLAKVIADMGRLGFALPTARGPWTPTH
                     AALDAAPAARWLREQGLHDPLLLTYLDYCCRDDYGAGIATVSAWALVHYFASRHGFSA
                     PGDDSNDRDPVLTWPDGNAPLARALRQPYADRVHTGRAVFRVDEGLGGVEAWAFAGDE
                     VEHWSARHLVMATPIFIAAHVLRAPPAALVEAAKAQRRAPWMVANLRLDALPLDRVGA
                     PLSWDNAIHGSPSLGYVDAQHQSLARVVGPRLLTAYWAFPDTDRKRLLQMSWQDATQL
                     VVDDLRAVHPDLSDRLLQVDAVRHGHAMAIPTPGLRSSPALAAVPASAARVSFAHADL
                     SAYSVFEEAFTRGEDAGQRVARALRGR"
     gene            complement(5551..7128)
                     /locus_tag="VIN75_00600"
     CDS             complement(5551..7128)
                     /locus_tag="VIN75_00600"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY8972684.1"
                     /translation="MRLRSLKADGGAAPRVGAGELALLASVFVVAACGLVYELCAGAL
                     ASYLLGDSVLQFSTVIGTYLFAMGLGSWASRHVRENLAGMFVRVELIVGVIGGLMPAA
                     LFAAHATLPAASMVSFKVLLYGLVTIVGIGVGLEIPIVMRLLDRHLKGRDTLSTLVSQ
                     VLTFDYLGSLVVSVAFPLLLVPHLGIVRSGVFFGLLNTLVAIWSMWLFRAEIPRWKWL
                     GAQCGAIFALLVAAMAAGDLLTTWSEDQFYGPGVVYHESTPYQRIVVTSQSGTLRLFL
                     NGNLQFNSKDEYRYHEALVHPAMAGYGAPRRVLVLGGGDGLAIREILKYPSVEQVTQI
                     ELDPAMTRLFTHDPTLAALNQHSLTSPKVRIVNADAFAWLEQHDDQFDVIVVDFPDPS
                     NYALGKLYSTSFYELVDQHLAAAGYAVVQTTSPLIARRSFWTVATTLETVGLHVVPYH
                     VHVPSFGEWGFLIASHRPWVLPRALPTGMRFLTLPGMQALLDFPPDMSRVPTEPNRLS
                     NQTLVRTFEEEWGKVHD"
     gene            complement(7128..7352)
                     /locus_tag="VIN75_00605"
     CDS             complement(7128..7352)
                     /locus_tag="VIN75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY8972685.1"
                     /translation="MNTPEWLHPEVIGGTVLYAVIGVVVLWLAFIIIDWITPYKLWEQ
                     IVEEKNLALAVMVGSMFIALGLIIAAAIHG"
BASE COUNT         1124 a         2606 c         2762 g         1183 t
ORIGIN      
        1 tgcccgcgcc gaagccgtag accgagtcgc tgaactgcag cgcctgctgc atctgcagct
       61 tcgcgaagcc cacgttgacg cgatcgagat aggccgcgat gtagcacagg aacagcagcg
      121 ggatgaggcg ccaggcgacc ttgcggtaga ccgcgtcggc gaccgcgtcg acctggcccg
      181 gctgcccctg cgagggcgcg gtgatgctgt tcatcgagcg tctcctgtct tgtgacgccc
      241 gcgatcctgc ggccggccgc gcgccgtggg cagcggggaa acacccattc cagccggtgt
      301 caggtcttga atcgtgcact cgactgcacg attcaagacc tgacaccgca actttcaaga
      361 cctgacaccg gcagatcgaa aaagcccgcc ggcatcgctg cgggcgggct tcgctttcag
      421 gacgacgcgg ggtcagttca cctgcttggt gaaggtgacc ggctcgatct cgacgcggcg
      481 gttcggttcc aggcacttga tcagcgcggc cttcttcttc tcgtccttgc actcgacgac
      541 cggatcggcc tcgcccttgc cgaccgcggt caggcggttg ccggcgacgc ccttgccctc
      601 gaggtaggcc ttgaccgcgt tggcgcgctt ctcggacagt tgctggttgt actgcttgct
      661 gcccaggcgg tcggtgtagc cgacgatggt gacgctggcg atgtcggcgt tggcgttcat
      721 cgtggcggcc acttcgtcga gcttgggctg gttcgggccc agcttggcgc tgtcgaagcc
      781 gaacagctcg ctggccgaca gcgtgtactt cttcatgacc ggcggcggcg ggggcagcgg
      841 agccggcggc ggcggcgggg gcggcggcgt ggtgacggtg gtggtctcga cggtgcgcgt
      901 ggtggtcgcc accggcaccg gctcgggcgc cggcgggccg ccgaaggccc aggtcaggcc
      961 cacgttggcg tacgtgttgg ccgtgtgctt catgtcgaac ggctgaacgc ggctggagta
     1021 gaacgtgccg acctcgcgga tgtcggcctg catgcccagc gtcggggtga acttgtactg
     1081 gaagcctagg ccggcgtcga cgtagggcgc cgtcttcttg aacgtgccgt tgacgccgtc
     1141 gccgaagtgg tcgcgcgcgg cgcccgcgcc gaacagcacg aacggacgga agccgcccgg
     1201 cgtgaacagg tacagcgcat cgacgcccgc gagcgtctgg tggaagttgg cgccgtggtc
     1261 ggtcgacgcg gcgtagttgc cgccgacctg caggtcccag ctcgggttca gcgggatgcc
     1321 caccttcagg ccgccgcccc agccggtctt gtcctccgga tacttggaat cgggcttgac
     1381 cgcgctgcca ttgagcagga tgtaggcgtt cgggttgtag gtctcgttgc tctgcgcgaa
     1441 agcatggccg gcgcaggcca gcgcggccgc cgccgcgagc gcgtcgagca cgcgcgattg
     1501 atgtcgagtc atggactcac tccttcttga agtcgtttga agaggtcacg gggagggccc
     1561 gccgggcgcc cgtgtgcgaa gcggaatcgg cggtgcccgt gaccctgcgt ctgccggagc
     1621 gcgcatcgtt gaagcattgc tggtccttct cggatgagtg gttccgaccg gggcagcgcc
     1681 acaacgtcga gccgggctcg acgtgggatt tggcaatcca cgttcccatt tcctttctgc
     1741 acaccgagtc acgacatgtg tcgacgcgtc gatgcgtcgt gcctcgtcga agggagaacg
     1801 tcggccgcag gctgtttctt ccgggcgccg atgtaacgac tcgttaggtc gagccggcca
     1861 tgagctcgac acggcctgca gcaatccttg cgcagcgggc cggtgctagg ctggcgcgat
     1921 gcgactccca cactcctgcg cagccctggc ggccgcgacg ttcctttgca gcgcgcccgc
     1981 ccgcggcgcc gagatcgtcc tcgaacagtc tgccgtccag aagctggtcg tcgaatcgct
     2041 gttcaaggac aacggccgct tctacatcca gcgcggcgcc tgcagcgcgt acctcgacaa
     2101 cgccgccgtc gcgctgacgg gcggccgcgt cgtcatccgc tcgcacctga gcgcgcgcgt
     2161 cggcatggac ctgggcgaca gctgcgccgg cgtcgacctc gcatcgtggg cgacggtctc
     2221 cggcgtgccg agcgcgcagg gcacgaccgt gcgcctgtcg gacatccgca tcgaggacgt
     2281 cggcgacgcg aacatgcgca tcgtgctgaa ctccgggctg gtgccgacgc tgccggcggc
     2341 cttcgagctg gacgtcctca aggccgtccg ctcgatgctc cagggcgcgg acagcccgct
     2401 ccaggtcgac gtgcaggcgc tgcgcatcga ctcggtgcgc gtcgccgaca accggctctc
     2461 ggtacgcttc gacttccgcg tcgccggccg ctgaacgcga ggccggcgat gcccgttcga
     2521 ctcattgcct cgagaacagg cccgtgatgc gctggcccag gcgctggaag aagccggcgc
     2581 gttccaccgc ggccagcgcg acgaccggcg tctcgcccag cggcttgccg ttggccgtgg
     2641 ccacgacctt gccgaccacg gcgcccttgg cgatcggcgc ctcgagcgtc gggttgagcg
     2701 tgaccgccgt ctgcacggcg ccggcctggc ccttgggcac cagcagcgtc caggccttcg
     2761 ggacgccgac ggccacggtg ggcaccatgc catacgtcac cttggcggtg gccagcgtcg
     2821 cgtcggcctt gacgggcgtc gcctgctcga aattggcgta gccccagttg aacagcacgc
     2881 gcgtctggtc ggcgcgcgcc tgggcgctgt cggcgttcat gatcaccgag atcaggcgca
     2941 agccgttgcg cttggccgag gccgtcaggc agtagccggc ttcctcggta tggccggtct
     3001 tgaggccatc gacggtcgga tccgtgtaca gcagcgcatt gcggttgccc tgcttgatgc
     3061 cgttgaacgt gaactcgtgc tccgcgtaga tcgggtagta ctcggcgctg ttgcggatga
     3121 tcgcctgcgc cagcacggca aggtcgcgtg ccgacgcctt gtggttcggg tcgggcaggc
     3181 cggccgaatt ctcgaagtgg gtatgcgtca tgcccagctt ggcggcttcg gcgttcatca
     3241 tggccacgaa gcccgattcg gagccgccga tgtgctcggc cagcgccttg gaggcgtcgt
     3301 tgccgctgtc gatgacgacg ccgcgcagca tgtccatcag cgaggcctgc gagttcagcg
     3361 gcacgaacat gcagctgtcg ccttccttgc cgcccttgca ccaggcgctc aggctcatcg
     3421 tgaccatctc ggtgggcttg agcttgccgc tcttgaggcc ctgctccacg aggtagcttg
     3481 tcatcatctt cgtgagcgac gcgggcggca gcggctcgtc ggcgttggac gacgccagga
     3541 tctgccccga gtcgaagtcc atcaggaccc acgccttgct ggccagcacc ggcaccgagc
     3601 gcccgttggc gtcggtcgtc atcgcgggtt cggtggactc ggccgccttg cgggccgcgg
     3661 cgtgcggctt ggccttgggc ttcgcatgcg tggcggcggt ggcggagccg gccgcggcga
     3721 ggaggatcgc ggcggcgagg gcgggaggaa gcaggcgggc ggtgcaggag cgggtggtca
     3781 tcggggaatt ctttgtcggg aagccgaagc gaggctccgc cagcgcggag ccgcgggaaa
     3841 cggcggggtg tccaggggcg tgctcgacag tgcgcgcgcg cggatcacgg cgtattgtga
     3901 cggacatgcg atcccgcccg cgcgggcaag ttcccgcggc aagcgcgtgt tcagcggccg
     3961 cgaagggcgc gcgccacgcg ctgcccggcg tcctcgccgc gcgtgaacgc ctcctcgaac
     4021 accgaatagg ccgacaggtc ggcatgcgcg aagctcacgc gcgcggcgct cgctggcacc
     4081 gcggcaagcg ccgggctcga gcgcaggccc ggcgtgggga tggccatggc gtggccatgg
     4141 cgcacggcgt ccacctgcag caggcgatcg gacaggtccg gatgcacggc gcgcaggtcg
     4201 tcgacgacca actgggtcgc gtcctgccac gacatctgca gcaggcgctt gcggtcggtg
     4261 tcggggaagg cccagtaggc ggtcagcagg cgcggcccga cgacgcgcgc gaggctctgg
     4321 tgctgcgcat cgacgtaacc cagcgacggg ctgccgtgga tcgcgttgtc ccaggacagc
     4381 ggcgcgccga cgcgatccag cggcagcgcg tccagccgca ggttggccac catccacggg
     4441 gcgcggcgct gggccttcgc agcttcgacg agcgccgccg gcggcgcgcg cagcacgtgc
     4501 gcggcgatga agatcggcgt cgccatgacc aggtgcctgg cgctccagtg ctcgacctcg
     4561 tcgcccgcga acgcccaggc ctcgacgccg cccagcccct cgtcgacgcg gaacaccgcg
     4621 cggcccgtgt ggacgcggtc ggcgtacggc tggcgcagcg cccgcgcgag cggtgcgttg
     4681 ccgtcgggcc aggtgagcac cgggtcgcgg tcgttcgagt cgtcgccggg cgcgctgaag
     4741 ccgtggcggc tggcgaagta gtgcaccagc gcccaggccg agacggtggc gatgccggcg
     4801 ccgtagtcgt cgcggcagca atagtcgagg taggtgagca gcagcgggtc gtgcaggccc
     4861 tgttcgcgca gccatcgcgc ggcgggcgcc gcgtcgagcg cggcatgggt gggcgtccac
     4921 gggccgcggg cggttggcaa cgcgaagccg aggcggccca tgtcggcgat gaccttggcg
     4981 agaaggcggt actgcgcctg cgtcgccgag tcgcgcggca ccagcggcaa catgccgtcc
     5041 tgccagcggc cgttgatgta gaggcgctcc tgcgggctgt ggcacaggtg gcgctcgtcc
     5101 cacttcaggc cggcggcggt ggacttcgcg agcccgatcc cgaccagcca gtcctggatg
     5161 tcgccggcct cgggcccggg cagcggcagg tagtgcgcac cgatcgggca gcgcaggcct
     5221 tgcatcgcgt ggccgcggct gttgccgcct ggctcgtcct cgagctccag caccgcgaag
     5281 tcggtgatgc ccgcgcgctg caacgtgcgc gccgccgaca gccccgcgat gcccgagccc
     5341 actatgagca cgtcgcagcg ccgctcgcgc gcgcccgcgg gcgggtgcgc ggccgcgtcc
     5401 cgtgcgcgat cgcgcacgcg atggcccagc gcgctgttgt cgccgaccca gcggccctcg
     5461 acgcgcgtgc gccagtcctc gggcttggcg cagccggtga ggatggccgg ggcggccagc
     5521 gcggcgccca ggaaggcgcg ccgcgaggtc tcagtcatgg accttgcccc actcttcttc
     5581 gaaggtgcgc acgagcgtct ggttcgagag gcggttcggc tcggtcggca cgcgcgacat
     5641 gtccggcggg aaatcgagca gcgcctgcat gcccggcagc gtgaggaagc gcatgcccgt
     5701 cggcagcgcg cggggcagca cccacggccg gtggctggcg atgaggaagc cccattcgcc
     5761 gaagctgggc acgtgcacgt gatagggcac cacgtgcagg cccaccgtct ccagcgtggt
     5821 ggccaccgtc cagaagctgc gccgcgcgat cagcggcgag gtcgtctgga cgaccgcgta
     5881 gccggccgcg gccaggtgct ggtcgaccag ctcgtagaag ctggtggagt agagcttgcc
     5941 gagcgcgtag ttgctggggt cggggaagtc gaccacgatc acgtcgaact ggtcgtcgtg
     6001 ctgctccagc catgcgaacg cgtcggcgtt gacgatgcgc accttgggcg aggtcagcga
     6061 gtgctggttc agcgccgcca gcgtcgggtc gtgcgtgaac agcctggtca tcgccgggtc
     6121 gagctcgatc tgcgtgacct gttcgaccga cgggtacttg aggatctcgc ggatcgccag
     6181 tccgtcgccg ccgcccagca ccagcacgcg ccgcggcgcg ccgtagccgg ccatcgccgg
     6241 gtgcaccagc gcctcgtggt accggtactc gtccttggag ttgaactgca ggttgccgtt
     6301 gaggaacagc cgcagcgtgc ccgactgcga ggtgacgacg atgcgctggt agggcgtgga
     6361 ctcgtgatag accacgcccg ggccgtagaa ctggtcctcc gaccacgtgg tcagcaggtc
     6421 gcccgcggcc atcgcggcca ccagcagcgc gaagatcgcg ccgcactgcg cacccagcca
     6481 cttccagcgc gggatctcgg cgcggaacag ccacatcgac cagatcgcca ccagcgtgtt
     6541 gagcagcccg aagaacacgc ccgagcgcac gatccccagg tgcggcacca gcagcagcgg
     6601 gaacgccacc gagaccacca gcgagccgag gtagtcgaag gtgagcacct gcgagaccag
     6661 cgtggacagc gtgtcgcggc ccttcaggtg gcggtcgagc agccgcatga cgatggggat
     6721 ctcgagcccc acgccgatgc ccacgatcgt caccagcccg tacagcagca ccttgaacga
     6781 gaccatgctg gccgccggca gcgtggcgtg cgccgcgaac agcgcggccg gcatgaggcc
     6841 gccgatgacg cccacgatca gctcgacgcg cacgaacatg ccggccaggt tctcgcgcac
     6901 atggcgcgag gcccaggagc ccagccccat cgcgaacagg taggtgccga tcacggtgga
     6961 gaactgcagc accgagtcgc cgagcaggta gctcgcgagc gcgccggcgc acagctcgta
     7021 cacgaggccg cacgcggcca cgacgaagac ggaggcgagc agcgcgagct cgcccgcgcc
     7081 gacgcgcggc gcggcgccgc cgtcggcctt cagggaacgc aggcgcatca accgtggatc
     7141 gcggcggcga tgatcaggcc cagcgcgatg aacatcgagc cgaccatgac ggccagcgcc
     7201 aggttcttct cctcgacgat ctgctcccac agcttgtagg gcgtgatcca gtcgatgatg
     7261 atgaaggcca gccacagcac gacgacgccg atcacggcgt acaggaccgt gccgccgatc
     7321 acctcggggt gaagccattc gggtgtgttc atctcgttct ccttgcttcg tctgtcacgt
     7381 cattcaaggc gtgctgacga ggtcagccgc ccaatcaagc tctcgggaag cgaggatcgc
     7441 atccgagccg aaccgagctc cccctcgggg ggcagcgacg ggaggagcgt gcgggctcat
     7501 ttgtgcccgc cgccaccgga cgaatacccg ccccaggcgc cgccggtgcc gtaatacgcg
     7561 cccgttcccg cggccgaacg cgccttgcat tgccggtatt ccgcgctgta cgagccgtag
     7621 gcgcgcttga cgtcgcggca cgagtcctgg ctgcaccacg ccagcagcat cagca
//
LOCUS       DATCRO010000024         6354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_213972, whole genome shotgun sequence.
ACCESSION   DATCRO010000024 DATCRO010000000
VERSION     DATCRO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213972"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1788)
                     /locus_tag="VIN75_00610"
     CDS             complement(286..1788)
                     /locus_tag="VIN75_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY8972686.1"
                     /translation="MSSLSPTAVGPLAAIDLGSNSFRLEIGQVQDGRYRRHLYLKETV
                     RLGAGLDVDGMLTEEATERGLKCLATFAQTLNASAPMQVRAVATQTLREARNRDKFLV
                     RAEEALGHPIEVISGREEARLIYAGVAFLQPSAKPRLVIDIGGRSTEMILGDGRAPRV
                     AESFQVGCVSLSMRYFPDGRYTRAAFRAAQVAAGAELEEALVPFARRHWREALGSSGT
                     AGAVADVLKASGKTDGRITADGLRWLIERCIELGHIDKLDIPGMKPERRAVIAGGLAI
                     LYTICAQFGIDELAPAKGALRQGVIVDLSERLAAGAQASGHDLRDTTIADLQRRFHVD
                     VAQAERVARTAQGFFDSTWPRTDAEMRQELRWAAALHEVGLMVSHHDHHRHSAYLVSH
                     VDAPGFSQSQQRRLGELVLGQRGGLRKIEAGLAREPTAWQVLCLRLAVIVCHARGEVD
                     ARGLKLARVGSGARLAFSPQWAEEHPRTAYLLREEADLWARQGVLLLTLG"
     gene            complement(1843..2766)
                     /locus_tag="VIN75_00615"
     CDS             complement(1843..2766)
                     /locus_tag="VIN75_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEY8972687.1"
                     /translation="MIGADPSHVSTVREFSRFYTRRLGTLDEGLLDTPWTLTEARVVF
                     ELAQAPSTDMAALRTTLAIDSGYLSRLLARFDSAGLIKRTASANDARRQSLSLTAAGR
                     KLFRTLDERSNRQVAQLLEPLGEAARESLVAGMRTVMRGLDEHPRPAVVELRGLRPGD
                     LGWVVQRHGETYAREHGWTMAFEALVARIMADYLEQHRPGRENAWIAEVDGQRAGCVL
                     CVRKDDETAQLRVLLVEAWARGHGLGARLVDECVRFARGCGYRKLVLWTNDVLGSARR
                     IYQAAGFVLVSEERHHSFGKDLVGQYWELRL"
     gene            complement(2825..3259)
                     /locus_tag="VIN75_00620"
     CDS             complement(2825..3259)
                     /locus_tag="VIN75_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8972688.1"
                     /translation="MYRSILIPTDGTDITAKAVDTGVALAKALGARLSTISVKEPFPY
                     SAISEMQPIPPQEFYDAQERIALNRVNEVVASAQAAGMECQAFTVEATHPWEAIVDHA
                     KTQSCDLIVMASHGRRGVSALLLGSETTRVLVHCDVPVLVVK"
     gene            complement(3345..4199)
                     /locus_tag="VIN75_00625"
     CDS             complement(3345..4199)
                     /locus_tag="VIN75_00625"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8972689.1"
                     /translation="MTTAALPTPLVSPEQLIAALAAGAAPLVIDCSFDLADLAAGERA
                     YAQGHLPGAIHAHLDRDLSTTKTGRNGRHPLPPRADWAATLARLGVTPARAVVVYDAQ
                     GGMYAARAWWMLLWAGHRDVRVLDGGLQAWRAAGGAVEAGVVAPVAAPVPYPLGDSLV
                     GSIDADTLQKSLGRVTVLDARAGERYRGEVEPLDARAGHIPGARSRFFKDNLDASGRF
                     RPAAELRAAFEAFGAAPAAVVHQCGSGVTACHNLLAMEAAGLSGSVLYPGSWSEWSSD
                     PARPIAVG"
     gene            complement(4201..5103)
                     /locus_tag="VIN75_00630"
     CDS             complement(4201..5103)
                     /locus_tag="VIN75_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8972690.1"
                     /translation="MSAPLLMICSSFLFATMGVCVKLASDTYGPGEIVMYRGLVGMIG
                     IALLARARGVPLRTSVPMMHAWRSVVGVAALTLWFYAIGHMPLATAVTLNYMSSVWMA
                     LFLIGGAVVVGAQRVDPRLVATVLVGFLGVALVLQPSVSRDQAVPGMMGLASGMLSAL
                     AYLQVTTLGRVGEPELRVVFYFSIGGFLTGLVMSLLQGWHGHHTPGGFLLLLATGVTA
                     TTAQVAMTRAYAIGRPLSNASLQYLGIAFSFVYGIVLFHDPLTWAAIGGMVLIVGAGL
                     AASRLRATTLAATAPDTIQPPSDT"
     gene            complement(5163..6278)
                     /locus_tag="VIN75_00635"
     CDS             complement(5163..6278)
                     /locus_tag="VIN75_00635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8972691.1"
                     /translation="MSDLSITLEALERARSQLSVSSYFDEDLFRREQELIFQHGPRYL
                     AHELAIPEIGDYYALPQEGEGRVLVRAADGGVELLSNVCRHRQAVMLKGRGQTASNIV
                     CPLHRWTYGLDGRLLGAPHFAQDPCLHLNRYRTRSWNGLVFEDNGRDVAADLARLGPR
                     AELDFAGYVLDKVHVHECDYNWKTFIEVYLEDYHVGPFHPGLGSFVTCDDLKWEFGAN
                     YSVQTVGVSNGLARPGSATYRKWHDALLRFNEGRPPRHGAIWLTYFPGIMVEWYPHVL
                     VVSTLYPKGPQQTTNVVEFYYPEEIAAFERDLVEAEQAAYLETCVEDDEIAQRMDSGR
                     KALFERGDDEAGPYQSPMEDGMRHFHEWYRHALKRGR"
BASE COUNT          957 a         2227 c         2262 g          908 t
ORIGIN      
        1 gcgccgatga tagtgcgggt tcccgtgtga aagtaggaca ttgtcaggct aatatcaaag
       61 caaccgcccg agctagtttt ctagctcggg cgttttgctt ttgggggcac gccgtcgcgt
      121 cacggtgtag ggcgggttag ccgcaggcgt aacccgccat gccggcatgt cgcgacgcca
      181 ccgaacggcg ggttacgcct gcggctaacc cgccctacgg aacaggggac gtgccaagga
      241 aaacgcccgg catgccgggc gttctgcatc aggccgtcaa tgcgctcaac cgagggtcag
      301 cagcagtacc ccttggcgtg cccacagatc cgcttcctca cgaagcaggt acgccgtccg
      361 cggatgctcc tcggcccact gcggcgagaa cgccaggcgc gcgcccgagc ccacgcgcgc
      421 gagcttgagg cctcgcgcat cgacctcgcc ccgggcgtgg cacacgatca ccgccagccg
      481 cagacacagc acctgccacg cggtcggttc gcgcgcgagc cccgcctcga tcttgcgcag
      541 gccgccccgc tggccgagca ccagttcgcc gagccggcgc tgctggctct gcgagaagcc
      601 gggcgcgtcg acgtgcgata cgaggtaggc gctgtggcgg tggtggtcgt ggtgcgacac
      661 catcaggccg acctcgtgca gtgcggccgc ccagcgcagt tcctggcgca tctcggcgtc
      721 cgtgcgcggc caggtgctgt cgaagaatcc ttgtgccgtc cttgccacgc gttcggcctg
      781 ggcgacatcg acatggaagc ggcgctgcag gtcggcgatc gtcgtgtcgc gcaggtcgtg
      841 gccgctcgcc tgcgcgcccg cggccaggcg ctcggacagg tcgacgatga cgccctggcg
      901 cagcgcgccc ttggcgggcg ccagttcgtc gatgccgaac tgcgcgcaga tcgtgtacag
      961 gatggccagg ccgcccgcga tcactgcgcg ccgctcgggc ttcatgccgg ggatgtcgag
     1021 cttgtcgatg tggcccagct cgatgcagcg ctcgatcagc cagcgcaggc cgtcggccgt
     1081 gatgcggccg tcggtcttgc cgctcgcctt caggacgtcg gcgacggcgc cggccgtgcc
     1141 cgacgagccc agcgcctcgc gccagtggcg gcgcgcgaac ggcaccaggg cctcctcgag
     1201 ctcggcgccc gccgccacct gggccgcgcg gaacgcggcg cgcgtgtagc ggccgtcggg
     1261 gaaatagcgc atcgacaggc tcacgcagcc gacctggaac gactcggcca cgcggggcgc
     1321 gcggccgtcg cccaggatca tctcggtgga gcgtccgccg atgtcgatca ccagccgtgg
     1381 cttggcagag ggctgcagga aggccacgcc cgcgtagatc aggcgcgcct cttcgcggcc
     1441 ggagatcacc tcgatcggat gcccgagcgc ctcctcggcg cgcacgagga acttgtcgcg
     1501 attgcgcgcc tcgcgcagcg tctgcgtcgc gactgcgcgc acctgcatcg gcgccgaggc
     1561 gttgagcgtc tgcgcgaagg tcgccaggca cttgaggccg cgttcggtgg cttcctcggt
     1621 gagcatgccg tcgacgtcga gtccggcgcc caggcgcacc gtctccttca ggtagagatg
     1681 gcgacggtag cgtccgtcct gcacctgccc gatctcgagg cggaagctgt tggagcccag
     1741 gtcgatggcg gccagcggcc cgacagcagt aggagagagg gaagacatgc gcgcgattgt
     1801 gccttccctg cgcggacgag cagatgtcag ccggcgcggt gcctacagcc gaagctccca
     1861 gtactggccg acgaggtcct tgccgaagct gtgatgtcgc tcctcgctga ccagcacgaa
     1921 gcccgccgcc tggtagatcc ggcgtgccga cccgagcacg tcgttcgtcc acagcaccag
     1981 cttgcggtag ccgcagccgc gcgcgaagcg gacgcattcg tcgaccaagc gcgctccgag
     2041 cccgtggccg cgcgcccagg cctcgacgag caggacacgc agctgggcgg tctcgtcatc
     2101 cttgcgcacg cacaggacgc agcccgcacg ctgcccatcc acctcggcga tccaggcgtt
     2161 ctcccggccg ggacgatgct gctcgaggta gtcggccatg atgcgcgcga ccagcgcctc
     2221 gaaggccatc gtccagccat gctcgcgggc gtaggtctct ccgtggcgct gcaccaccca
     2281 gccgagatcg cccgggcgca ggccgcgcag ctcgacgacc gcggggcgcg ggtgctcgtc
     2341 gaggccgcgc atcaccgtgc gcatgccggc caccagcgac tcccgcgccg cctcccccag
     2401 cggctccagc aactgcgcca cctgtcggtt ggagcgctcg tcgagcgtgc ggaagagctt
     2461 gcggcccgcc gccgtgagcg acagggactg gcgacgcgcg tcgttggccg acgccgtgcg
     2521 cttgatcagg cctgcggagt cgaacctggc cagcaaccgg ctgaggtagc ccgagtcgat
     2581 ggccagcgtc gtgcgcagcg cggccatgtc ggtggacggg gcctgcgcga gctcgaagac
     2641 gactcgcgcc tcggtgaggg tccagggcgt gtcgagaagg ccctcgtcca gcgtgccgag
     2701 ccggcgcgtg tagaagcgcg agaactcgcg cacggtcgag acgtgggaag ggtcggcgcc
     2761 gatcatctga ctgagtccgc tagattgctg actcggtcag tttaatgcga ccggaggctg
     2821 ggtgctactt cacgaccagc acgggcacgt cgcaatgcac cagcacgcgc gtggtctcgc
     2881 tgcccagcag gagcgcactg acgccgcggc ggccgtgcga ggccatcacg atcaggtcgc
     2941 aggactgcgt cttggcatgg tcgacgatcg cctcccacgg gtgcgtcgcc tcgacggtga
     3001 aggcctggca ctccatgccc gcggcctgtg ccgacgccac cacctcgttg acccggttga
     3061 gcgcgatgcg ctcctgcgcg tcgtagaact cctgcggcgg gatgggctgc atctcggaga
     3121 tcgcgctgta cgggaacggc tccttcacgc tgatcgtgct gagccgggcg ccgagcgcct
     3181 tggcgagggc gacgcccgtg tcgacggcct tggccgtgat gtcggtgccg tcggtcggaa
     3241 tgaggatgct gcggtacatg ccggtctcct gcagtggcga ccaagccagt gtgcgccttt
     3301 cgcgcgcggc ccgcagcccc ggcttcaccc gggtttgacc gcgctcaacc gaccgcgatg
     3361 gggcgcgccg gatcggacga ccattcgctc cacgagcccg ggtacagcac cgagccggac
     3421 agcccggcgg cctccatcgc gagcaggttg tggcatgccg tgacccccga gccgcactgg
     3481 tgcacgaccg cggccggcgc cgcgccgaag gcctcgaagg cggcgcgcag ttcggcggcc
     3541 gggcggaagc gaccgctcgc atcgaggttg tccttgaaga agcgcgagcg ggcgccgggg
     3601 atgtggccgg cgcgcgcgtc cagcggctcg acctcgccgc gatagcgctc gcccgcgcgg
     3661 gcgtcgagca ccgtcacgcg gcccagcgac ttctgcaggg tgtcggcgtc gatgctgccc
     3721 accaggctgt cgcccagcgg gtaaggcacc ggtgcggcga cgggggcgac gacgccggcc
     3781 tcgaccgcgc cgccggccgc ccgccacgcc tgcaggccgc catcgagcac gcgcacgtcg
     3841 cgatggcccg cccacagcag catccaccag gcgcgcgctg cgtacatgcc cccctgggcg
     3901 tcgtagacga ccaccgcgcg cgcgggcgtg acgcccaggc gcgcgagcgt cgcggcccag
     3961 tcggcgcgcg ggggcagcgg atgacggccg ttgcggcccg tcttcgtcgt ggacaggtcg
     4021 cggtcgaggt gcgcgtggat cgcgcccggc aggtggccct gcgcgtaggc gcgctcgccg
     4081 gcggcgaggt ccgccaggtc gaagctgcag tcgatcacca gcggcgcggc gccggccgcg
     4141 agcgcggcga tcagttgctc gggcgagacc aggggcgtgg gcagggcggc agtcgtcatg
     4201 tcaggtgtcc gagggcggtt ggatggtgtc gggggcggtg gcggccagcg tcgtggcgcg
     4261 caggcgcgag gccgcgaggc cggcgccgac gatcagcacc atgccgccga tggccgccca
     4321 cgtgagcgga tcatggaaca gcacgatgcc gtagacgaac gagaacgcga tgcccaggta
     4381 ctgcaggctc gcgttggaca gcggccgccc gatcgcgtac gcgcgggtca tggccacctg
     4441 cgcggtggtc gcggtgacgc cggtggccag cagcagcagg aagccgccgg gcgtgtgatg
     4501 cccatgccag ccttgcagca gcgacatcac caggccggtc aggaagccgc cgatcgagaa
     4561 gtagaagacg acgcgcagct cgggctcgcc gacccggccc agcgtcgtca cctgcaggta
     4621 ggcgagcgcg gacagcatgc ccgacgcgag tcccatcatc ccgggcaccg cctggtcgcg
     4681 gctgacggac ggctgcagca ccagggccac gcccaggaag ccgaccagca ccgtggccac
     4741 cagccgcggg tccacgcgct gcgcgcccac cacgacggcg ccgccgatca ggaacagcgc
     4801 catccagacc gacgacatgt agttgagcgt gaccgccgtg gccagcggca tgtggccgat
     4861 cgcgtagaac cacagcgtga gcgcggcgac gccgacgacg ctgcgccacg cgtgcatcat
     4921 cggaacgctc gtcctgagcg ggacgccgcg cgcgcgtgcc agcagcgcga tgccgatcat
     4981 gcccaccagg ccgcggtaca tcacgatctc gcccggcccg taggtgtccg acgcgagctt
     5041 cacgcacacg cccatcgtcg cgaacaggaa cgacgagcag atcatcagca agggagcgga
     5101 catcggccca ttctccctcg cgaccgcgtc gtcccgctcg gcgaggggca ctcgacgccc
     5161 gttcagcgcc cccgcttgag cgcgtgccgg taccactcgt ggaagtggcg catgccgtct
     5221 tccatcgggc tctggtacgg gccggcctcg tcgtcgccgc gttcgaacag cgccttgcgg
     5281 ccggagtcca tgcgctgcgc gatctcgtcg tcctcgacgc aggtctccag gtaggcggcc
     5341 tgctcggctt ccacgaggtc gcgctcgaac gccgcgatct cctcggggta gtagaactcg
     5401 accacgttgg tcgtctgctg cgggcccttc gggtacagcg tggagacgac cagcacgtgc
     5461 gggtaccact ccaccatgat gcccgggaag taggtgagcc agatggcgcc gtggcgcggc
     5521 ggccggcctt cgttgaagcg cagcagcgcg tcgtgccact tgcggtaggt ggcgctgccc
     5581 ggccgcgcga ggccgttgga cacgcccacc gtctgcaccg agtagttggc gccgaactcc
     5641 cacttcaggt cgtcgcaggt gacgaagctg cccaggcccg gatggaacgg gccgacgtgg
     5701 tagtcctcga gatagacctc gatgaaggtc ttccagttgt agtcgcactc gtgcacgtgc
     5761 accttgtcga gcacgtagcc ggcgaagtcg agctcggcgc gcggcccgag gcgggcgagg
     5821 tcggcggcga cgtcgcggcc gttgtcctcg aacacgaggc cgttccagct gcgcgtccgg
     5881 tagcggttca ggtgcaggca aggatcctgc gcgaagtgcg gcgcgcccag gagccggccg
     5941 tcgagcccgt acgtccagcg gtgcagcggg cagacgatgt tggacgccgt ctgcccgcga
     6001 cccttgagca tgaccgcctg ccggtgccgg cacacgttgg acagcagctc gacgccgcca
     6061 tcggccgcgc ggacgagcac gcgcccttcg ccttcctgcg ggagggcgta gtagtcaccg
     6121 atttcgggta tcgccagctc gtgcgccaga tagcgcggtc cgtgctggaa gatgagttcc
     6181 tgttcgcggc ggaacaggtc ctcgtcgaag taggaagaaa cggaaagttg agagcgcgcg
     6241 cgctccagag cttctagcgt gatgctcagg tccgacatga tccctctgga tcccaaaaaa
     6301 ctgtcacaac cccccacccg gtggccccgc gggtggatct gtacgaatcg tcgt
//
LOCUS       DATCRO010000025         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_754038, whole genome shotgun sequence.
ACCESSION   DATCRO010000025 DATCRO010000000
VERSION     DATCRO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754038"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VIN75_00640"
     CDS             complement(<1..1032)
                     /locus_tag="VIN75_00640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY8972692.1"
                     /translation="MTARTLYDKLWDDHVLHTEDDGTSLLYIDRHLLHEVTSPQAFEG
                     LEMADRKVWRLSANLAVSDHNVPTTDRSQGIVDPTSRLQVDTLDRNCDRQGITQFKMN
                     DKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLL
                     AKKAKNMLVRVEGTLARGCSAKDIVLAIIGRIGTAGGTGYTIEFGGSAIRALSMEGRM
                     TVCNMAIEAGARAGLVAVDDTTLAYVKGRPFSPQGVEWDQAVAYWRTLHSDAGAQFDK
                     VVELRAEEIRPQVTWGTSPEMVVSIEDRVPDPEKEKDSNKRNAMERALEYMNLQPNTP
                     MESINIDKVF"
     gene            complement(1154..2098)
                     /locus_tag="VIN75_00645"
     CDS             complement(1154..2098)
                     /locus_tag="VIN75_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972693.1"
                     /translation="MRFDLVTLNLVLAIAETRSITRGAEREHLALAAASKRLSDLEAR
                     LGVGLFDRRARGVDLTEAGRALVRHIRTLNASLHALESEVVEFSRGIKGHLRVVANAG
                     AISECLPADLAAFARAHPGIRVSLEDQTSADVMQAVAEGRADVGIFVGPSTDARLAAR
                     HYNTGTLAAIVPADHILARRDAVPFDALLDFDIVGLHVGASAHELMLKSALERGRTLN
                     ARLQVRGFDAIAQLVAAGLGVAVLPADTAARFSKLFDIKVLALEEAWARRDYLLATRV
                     SDSLPAAVQRFVDALCPPSAATPASAPAAPRAREAAAT"
BASE COUNT          369 a          866 c          948 g          403 t
ORIGIN      
        1 gaacaccttg tcgatgttga tcgactccat cggcgtgttc ggctgcaggt tcatgtattc
       61 cagcgcgcgc tccatggcgt tgcgcttgtt cgagtccttt tccttctcgg gatcgggcac
      121 gcggtcctcg atgctgacca ccatctcggg cgaggtgccc caggtgacct gcggacggat
      181 ctcctcggca cgcagctcca ccaccttgtc gaattgcgcg ccggcgtccg aatgcagggt
      241 gcgccagtag gccacggcct ggtcccattc cacgccctgg ggcgagaagg ggcggccctt
      301 gacgtaggcc agggtggtgt cgtcgacggc gaccaggccg gcgcgcgcgc ccgcctcgat
      361 ggccatgttg cacaccgtca tgcgcccttc catcgacagc gcgcggatcg ccgagccgcc
      421 gaactcgatc gtgtagccgg tgccgccggc ggtgccgatg cggccgatga tggccaggac
      481 gatgtccttg gcgctgcagc cgcgcgcgag cgtgccctcg acgcgcacca gcatgttctt
      541 cgccttcttc gccagcagcg tctgcgtcgc gagcacgtgc tcgacctcgc tggtgccgat
      601 gccgtgcgcc agcgcgccga acgcgccgtg cgtggaggtg tggctgtcgc cgcagaccac
      661 ggtcatgccc ggcagcgtgg cgccctgctc cggcccgatc acgtggacga tgccctggcg
      721 cttgtcgttc atcttgaact gcgtgatgcc ttgccggtcg cagttcctgt ccagcgtgtc
      781 gacctgcagg cgcgacgtcg ggtcgacgat gccctggctg cggtcggtgg tgggcacgtt
      841 gtggtcgctg accgcgaggt tggccgacag gcgccacacc ttgcggtcgg ccatctccag
      901 gccttcgaag gcctgcgggc tggtgacctc gtgcagcagg tggcggtcga tgtacagcaa
      961 cgaggtgccg tcgtcctcgg tgtgcaggac gtggtcgtcc cagagcttgt cgtaaagggt
     1021 gcgggcggtc attggagccc ctcccgcgac gaggtcgtcg ctccgtcccc cgaggggact
     1081 gcgggccttc cgggggcggc ccggcgcacg gcccgcaacg aagtttgcac tgcgtgattc
     1141 atgagaaacc tcttcaggtg gcggcggcct cgcgcgcgcg cggcgcggcg ggcgcgcttg
     1201 cgggcgtggc ggccgatggc gggcacagcg cgtcgacgaa gcgctgcacg gcggccggca
     1261 gcgaatcgct cacgcgcgtg gcgagcaggt agtcgcggcg ggcccacgct tcctcgaggg
     1321 ccagcacctt gatgtcgaac agcttggaga aacgggcggc ggtatcggcc ggcagcaccg
     1381 cgacgcccag gccggcggcc acgagctgcg cgatggcgtc gaagccgcgc acctgcagcc
     1441 gcgcgttgag cgtgcggccg cgctcgagcg cggacttgag catcagctcg tgcgccgacg
     1501 cgcccacgtg caggccgacg atgtcgaagt ccagcagcgc gtcgaacggc accgcgtcgc
     1561 ggcgcgcgag gatgtggtcg gccggcacga tggcggccag cgtgccggtg ttgtagtggc
     1621 gcgcggccag gcgggcgtcg gtgctggggc cgacgaagat gcccacgtcg gcgcgccctt
     1681 cggccacggc ctgcatcacg tcggcgctgg tctggtcctc caggctcacg cggatgcccg
     1741 ggtgggcgcg cgcgaaggcc gcgaggtcgg ccggcaggca ttcggagatc gcgcccgcgt
     1801 tggcgaccac ccgcaggtgg cccttgatgc cgcgcgagaa ctcgacgacc tcgctctcga
     1861 gcgcgtgcag cgacgcgttg agcgtgcgga tgtggcgcac cagcgcgcgc cccgcctccg
     1921 tgaggtcgac gccgcgggca cggcggtcga acaggccgac accgaggcgc gcctcgaggt
     1981 cggacagccg cttgctggcg gccgccagcg ccaggtgctc gcgttcggcc ccccgcgtga
     2041 tgctgcgtgt ctcggcgatg gcgagaacga gattgagcgt gacgagatcg aagcgcatgg
     2101 gaccggactc tggctgactg gtgggaactg cgaagagtca gctgtgactg acgcggaacg
     2161 gcccgatcgg cggacctcac accggtaaac gtggatccgc catcggtccg gaagcgccgg
     2221 ataggtgctc cgatcgccga accgctgtcg caatgatgcg cgaagggtcg gcgaagcgcg
     2281 cgcggctgtg atgaattcag ccttcttcgc aaacgaagtc ttatcgtacc gctgccggag
     2341 gtcccatgtc gaggactttg catgtgtggt tggcaggtcg cgccggcccc tgctgaggcc
     2401 aagccggcac gaaacacccc tcgcacgcat gcgtgcgcgg acgcccccgc ggaccgcaag
     2461 gacgaaaaaa agcctcgcag cagcaagcgc gaggctgtga acggagcagg aagcgccgcg
     2521 aagggcgcgc caggctctgg aggttcaggc gctgcggcgg gccgtcacga ccacgcgctg
     2581 cacggc
//
LOCUS       DATCRO010000026         8083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_540326, whole genome shotgun sequence.
ACCESSION   DATCRO010000026 DATCRO010000000
VERSION     DATCRO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540326"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VIN75_00650"
     CDS             complement(<1..515)
                     /locus_tag="VIN75_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972694.1"
                     /translation="MNESAAAWAYTGLSRTLRDAALTVLAMAGAVAGSQAVAHGPAAG
                     VLGAVLALSLSRSHLVAGRHGWLEAAVLLPVVSLASHGVGALLLHVPWLGAAAFVVAG
                     SVPAWARRLGTTGRRLGRLLTLPLVTILVVPGLPLRPAWGLPPAFAAVAPAVIALIAL
                     ACVMLVQGLGKA"
     gene            760..1992
                     /locus_tag="VIN75_00655"
     CDS             760..1992
                     /locus_tag="VIN75_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8972695.1"
                     /translation="MTLAIRLSAVALACMAATAFAAEPIKIGVDGPFTGGSSSMGVSM
                     RDGVRLAADEINKAGGVLGRQIQLVERDDEAKNERGAQIAQELINKEHVVAVVGYINT
                     GVGLASERFFQDAKIPVMNNVATGSVLTHQFDDQPENYVFRNSANDSIQAPMIVEEAI
                     TRRGFKKVAILADSTNYGQLGRADLEKALALKGVKPVAVEKFNIKDVDMTAQLLKAKE
                     AGAEVVLTYGIGPELAQIANGMTKLGWKVPMIGSWTLSMANYIDNAGPGGEGARMPQT
                     FIQEPTTPKRQSFIISYLKTFAPKNNRMDSPVSAAQGYDSVYLLAAAIKQAGSTDGPK
                     IREALENLKTPVEGVVTTYNKPFSKGDHEAITANIPVFGEVKGNRVVYAYADDFKKAS
                     EVRVKDVNAKGALPTVKK"
     gene            2075..2950
                     /locus_tag="VIN75_00660"
     CDS             2075..2950
                     /locus_tag="VIN75_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8972696.1"
                     /translation="MEILTQLIFSGIALGMIYAVIAFGYQLTFATSDTLNFGQGDALM
                     LGALVGLTLVGMGVNYWLMIPIVCVFGAIQGAVVERIGVRPAIRIKSEFGWIMSTIAL
                     GIIFKNVAENVWGHDDLKFPSPLPESPLRLLGANVLPMEILVVVGAVLMMAAVELFNR
                     KTIWGKAVVATFNDREAARLMGINTGLVITFSYALSSMSAALAGVMIAPLTLTGATMG
                     AVLGLKAFAVAIIGGLTSGMGIVVGGIILGIAEKLTGFYLSTGYQDVPGLVLLLIVLA
                     VRPAGLFGKTAIKKV"
     gene            2960..4888
                     /locus_tag="VIN75_00665"
     CDS             2960..4888
                     /locus_tag="VIN75_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY8972697.1"
                     /translation="MKSRPLLLAAVGLALLVGFPFVVANPYYIHLAQTIAIYAILLFG
                     LDIVVGYTGQVSLGHAGLFGIGAYTAGVLYMHFHAPLLLTIVCGIGATAAFGAILALP
                     ALRVTGPYLAMVTLAFGTIIQILINEVTDVTNGPMGITFSKPVLLGMVLDERAYYWVC
                     SILLVASLIVVHRLLRSHLGRAFEALRGSPIASDCMGVSVYRYKVYAFVISAGFAGLA
                     GSLYAYSERYISPNTYNFELTVLFLLAIIMGGRKTRSGALLGATIIVMLPKLLDDLAL
                     FRYVSVTLAVLVTAGAGLLVARGKSTLARSAIPVAGSVALAVLSFILTSMTDWRLSIF
                     GLITLLVVYYLQDGIVGYVRQTLGWRGHGADDAAQAAALAAPASAVSTATAAGTGELV
                     AADKVLMQFGGLKALNEVTLHVARGSIHGLIGPNGSGKSTMMNVLTGIYVPTAGAIAF
                     KGQSIVGRTSARIALAGIARTFQNVQLFGEMTALQNVLVGLHHSYRSNLLDVALHLPR
                     FRREEREGTARAYALLKFVGLESLAGEEARNLPYGKQRLLEIARALALNPELLLLDEP
                     AAGLTAPDIKELVVIIRKIREHGITLILIEHHMDVVMSVCDVVTVLDFGQKIAEGTPA
                     AVQSDPQVIKAYLGSEAH"
     gene            4890..5612
                     /locus_tag="VIN75_00670"
     CDS             4890..5612
                     /locus_tag="VIN75_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8972698.1"
                     /translation="MLTLTNLHAGYGKVEVLHGISLEVPKGQVVTLIGSNGAGKTTTM
                     RAISGMIAPREGKVMLGDVDITGRDSHRIARLGLAHSPEGRRVFATMSVNDNLLLGAF
                     PRYTGARAKGDIAADLERAMEYFPRLKERRTQLAGTLSGGEQQMLAMARAMMMNPDVV
                     LLDEPSMGLAPILVDEVFRIISRLRAEGITMLLVEQFAAAALNVADYGYVLENGRIAV
                     HGPAEKLRNDPAVQAAYLGGGH"
     gene            complement(5632..>8083)
                     /locus_tag="VIN75_00675"
     CDS             complement(5632..>8083)
                     /locus_tag="VIN75_00675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8972699.1"
                     /translation="SGGADGGAVPVDSLYEDAHGQIWIGTNLHGVLVLDPASGRVSRL
                     QPAGASDPGLLTDSATSFEENGNGEIWIGTSTRGLLIADTDSLQARRVIRDRSVPSNL
                     QANWIWGLHRDRGGEMWVASTTGLSRTRPQQDAILSLFSSDGSAWTDPYVFSVDVDSR
                     GVVTLGQKNGIEVIDPIEQKMTALLGGSEDDPAHPPAISNATAVVHTADGTRWMASRD
                     GLFRLAPGARKLETIAMPERGAVKGVIALEVDGSTLWVGGKGDGIWQLDLDSPTHAMR
                     QPVPTSALTDPEVNAIVHGPDGAMWIATNNGLNLLRPGQPVQRIMPSANDPTGLSPAV
                     INTLLTDRQGRLWVGNNAGVQILEKFDAGGHAVFHHIGKDDGLTSENIDTMLMDAIGR
                     VWISTDDGVAVVDPSTYALRVLHPADGAAISTYWVNSGAVGPTGELFFGGLGGLTVIR
                     PERLTHRVYSAPLVVTDVEMGGKPAALADASGRKQLTVPPEAGGFSVEFSLLDFTSPE
                     RNRYAYWLEGYDKTWREVDTTHRSAGYTNLSPGRYVLHMKAASRDGAWTERPLEVDVE
                     ILPAWYQTWWFDLLKIIAVLLVIAAVAAVRVRYVQQRSKELARLVEQRTQELTKLQHQ
                     LEELAYSDALTGLPNRRMFGDYCRQMIAAAERQDSGFALLLIDLDHFKQVNDTLGHDA
                     GDALLVETARRLRGIARKADVVARLGGDEFAILLADTGVPSAVDIVCHRICEAFDAPI
                     PHAGHDMRTSPSIGVALFPSDGTTQDELYKSADVALYEAKREGRHTWRFAQRKARATL
                     TRLGGAASAGTGTDGPAD"
BASE COUNT         1225 a         2831 c         2802 g         1225 t
ORIGIN      
        1 gcctttccca ggccttggac cagcatgacg caggccagcg cgatcaatgc gatcaccgca
       61 ggcgcgaccg ccgcgaaagc gggcggcagg ccccaggcgg gccgcagcgg caggcccggc
      121 acgacgagga tcgtgacgag cggcagcgtc agcaggcggc ccaggcgccg gcccgtcgtg
      181 cccaagcgcc gcgcccacgc gggcaccgac ccggcgacga cgaacgccgc cgcgccgagc
      241 cagggcacat gcagcagcag cgcacccacg ccgtggctcg cgaggctgac gaccggcagc
      301 agcacggcag cctccagcca gccgtggcgt ccggcgacga gatggctgcg cgaaagcgaa
      361 agcgccagca ccgcgcccag cacgccggcc gccggcccgt gcgcgaccgc ctgcgagccg
      421 gccacggcac cggccatggc gagcacggtc agcgcggcgt cgcgcagcgt gcgcgacaac
      481 ccggtgtatg cccaagcggc ggcggactcg ttcaaatggt gaacctcgcg agctggcgac
      541 gggccgattc tcgccccgaa ctgacgcggg tcttacacgg gcgccgagcc cgcgcggcga
      601 gcgtggcctc cgcaccgaaa tggctctcgg ggtttgcacg aatctgcggg gattacgtag
      661 gcgcaaacgg tgaccggagc accacaatcg ccttcacatc gactcgcccc gagtcacaac
      721 gcgaggcaac agcagcttgc cccaatggag acacgaccca tgactctcgc catccgcctt
      781 tccgccgtcg ccctggcctg catggccgcc accgccttcg ccgccgagcc gatcaagatc
      841 ggtgtcgacg gaccgttcac ggggggctct tcctcgatgg gcgtgagcat gcgcgacggc
      901 gtgcgcctgg ccgccgacga gatcaacaag gccggcggcg tcctgggccg ccagatccag
      961 ctcgtcgagc gcgacgacga ggccaagaac gagcgcggcg cgcagatcgc ccaggagctg
     1021 atcaacaagg agcatgtcgt cgccgtggtg ggctacatca acaccggcgt gggcctggcc
     1081 tccgagcgct tcttccagga cgcgaagatc ccggtcatga acaacgtggc caccggctcg
     1141 gtgctcaccc accagttcga cgaccagccc gagaactacg tcttccgcaa ctcggccaac
     1201 gactcgatcc aggcgccgat gatcgtcgag gaggcgatca cccgccgcgg cttcaagaag
     1261 gtggccatcc tcgccgactc gaccaactac ggccagctcg gtcgcgccga cctcgagaag
     1321 gcgctcgcgc tgaagggcgt caagccggtg gcggtcgaga agttcaacat caaggacgtc
     1381 gacatgacgg cccagctgct caaggccaag gaagccggcg cggaagtcgt gctcacctac
     1441 ggcatcggcc ccgagctggc gcagatcgcc aacggcatga ccaagctggg ctggaaggtg
     1501 ccgatgatcg gcagctggac gctgtcgatg gccaactaca tcgacaacgc cggccccggc
     1561 ggcgaaggcg cgcgcatgcc gcagaccttc atccaggagc cgacgacgcc caagcgccag
     1621 tccttcatca tctcgtacct gaagaccttc gcgccgaaga acaatcgcat ggactcgccg
     1681 gtgtcggcgg cccagggcta cgattcggtc tacctgctgg ccgccgccat caagcaggcc
     1741 ggctccaccg acggcccgaa gatcagggaa gcgctcgaga acctgaagac gccggtcgaa
     1801 ggcgtggtca ccacgtacaa caagccgttc tccaagggcg accacgaggc catcaccgcc
     1861 aacatcccgg tgttcggcga ggtcaagggc aatcgcgtcg tctacgcgta cgcggacgac
     1921 ttcaagaagg cctccgaggt gcgcgtgaag gacgtcaacg ccaagggtgc gctgcccacg
     1981 gtgaagaagt aagctcctcc tcgcgcatgc atgacgcgcc gccccgacgc accgtcgcgg
     2041 cggcgcttct cttgaatcac cggcgggccc gcccatggaa atcctcaccc aactgatctt
     2101 cagcggcatc gcgctgggca tgatctacgc ggtcatcgcg ttcggctacc agctcacctt
     2161 cgccacgtcg gacaccctca acttcggcca gggcgacgcg ctgatgctgg gcgcgctggt
     2221 gggcctgacg ctggtgggca tgggggtcaa ctactggctg atgatcccca tcgtgtgcgt
     2281 gttcggagcg atccagggcg cggtcgtcga gcgcatcggc gtgcggccgg ccatccggat
     2341 caagagcgag ttcggctgga tcatgtcgac catcgcgctg ggcatcatct tcaagaacgt
     2401 ggccgagaac gtgtggggcc acgacgacct gaagttcccg tcgccgctgc ccgagtcgcc
     2461 gctgcggctg ctgggcgcga acgtcctgcc gatggagatc ctcgtggtgg tcggcgccgt
     2521 cctgatgatg gcggcggtgg agctgttcaa ccgcaagacg atctggggca aggccgtggt
     2581 ggccaccttc aacgaccgcg aggccgcgcg cctcatgggc atcaacacgg ggctggtgat
     2641 cacgttctcg tacgcgctgt cgtcgatgag cgcggcgctc gccggcgtga tgatcgcacc
     2701 gctgacgctc accggcgcca ccatgggcgc ggtgctgggg ctgaaggcct tcgccgtggc
     2761 catcatcggg ggcctgacca gcggcatggg catcgtcgtg ggcggcatca tcctgggcat
     2821 cgcggagaag ctcaccggct tctacctgtc caccggctac caggacgtgc ccggcctggt
     2881 gctgctgctg atcgtgctgg ccgtgcggcc ggcgggcctg ttcggcaaga cggcgatcaa
     2941 gaaggtctga gagtccgaga tgaaatcgcg ccccctgctg ctcgccgccg tcggcctcgc
     3001 cctgctcgtc ggcttcccgt tcgtcgtcgc caacccgtac tacatccacc tcgcgcagac
     3061 catcgccatc tacgcgatcc tgctgttcgg gctcgacatc gtcgtcggct acacggggca
     3121 ggtgtcgctg ggccatgccg ggctgttcgg catcggcgcc tacacggccg gcgtgctcta
     3181 catgcacttc cacgcgccgc tgctgctgac gatcgtctgc ggcatcggcg cgacggccgc
     3241 cttcggcgcg atcctcgcgc tgccggcgct gcgcgtcacg ggcccctacc tcgcgatggt
     3301 gacgctggcc ttcggcacca tcatccagat cctgatcaac gaggtcaccg atgtcaccaa
     3361 cggcccgatg ggcatcacct tctccaagcc cgtgctgctg gggatggtgc tggacgagcg
     3421 cgcctactac tgggtctgct cgatcctgct ggtggcctcg ctgatcgtgg tgcaccggct
     3481 gctgcgctcg cacctgggac gcgccttcga ggccctgcgc ggcagcccga tcgcgtcgga
     3541 ctgcatgggc gtctcggtct accgctacaa ggtctacgcg ttcgtcatca gcgccggctt
     3601 cgccgggctc gccggctcgc tgtacgcgta ctcggagcgc tacatctcgc ccaacacgta
     3661 caacttcgag ctgacggtgc tgttcctgct ggccatcatc atgggcggcc gcaagacgcg
     3721 ctccggcgcg ctgctgggcg caaccatcat cgtgatgctg cccaagctgc tcgacgacct
     3781 cgcgctgttc cgctacgtgt cggtcacgct ggccgtgctg gtgacggcag gcgcggggct
     3841 gctggtggcg cgcgggaagt cgacgctcgc gcgctccgcc attcccgtgg ccggcagcgt
     3901 cgcgctggcc gtgctgtcgt tcatcctgac ttcgatgacc gactggcgcc tgtccatctt
     3961 cggcctgatc acgctgctgg tcgtctacta cctgcaggac ggcatcgtcg gctacgtgcg
     4021 ccagacgctc ggctggcgcg gccatggtgc cgacgatgcc gcccaggccg ccgcgcttgc
     4081 cgcgccggcc agcgccgtgt cgaccgcgac ggcggcgggc acgggcgaac tggtcgcggc
     4141 cgacaaggtg ctgatgcagt tcggcggcct gaaggccctc aacgaggtca cgctgcatgt
     4201 ggcgcgcggc agcatccacg ggctgatcgg ccccaacggt tcgggcaaga gcacgatgat
     4261 gaacgtgctc accggcatct acgtgcccac ggcgggcgcg atcgccttca agggccagtc
     4321 catcgtcggc cgcacgtcgg cgcgcatcgc gctggcgggc atcgcgcgca ccttccagaa
     4381 cgtgcagctg ttcggcgaga tgacggcgct gcagaacgtg ctcgtgggcc tgcaccactc
     4441 ctaccgcagc aacctgctgg acgtggcgct gcacctgccg cgcttccggc gcgaggagcg
     4501 cgagggcacg gcgcgcgcct acgcgctgct gaagttcgtg ggcctcgaat cgctggccgg
     4561 cgaggaggcg cgcaacctgc cctacggcaa gcagcgcctg ctggagatcg cgcgcgcgct
     4621 ggcgctcaac ccggagctgc tgctgctgga cgagcccgcc gccggcctga cggcgcccga
     4681 catcaaggag ctggtcgtca tcatccgcaa gatccgcgag cacggcatca cgctgatcct
     4741 catcgagcac cacatggacg tggtgatgtc ggtgtgcgac gtcgtcaccg tgctggactt
     4801 cggccagaag atcgccgaag gcacgccggc cgcggtgcag tccgatcccc aggtcatcaa
     4861 ggcctatctt ggttcggagg cccactgaca tgctcacgct cacgaacctg cacgccggct
     4921 acggcaaggt cgaggtcctg cacggcatct cgctggaggt gcccaagggg caggtcgtca
     4981 cgctgatcgg cagcaacggc gccggcaaga cgaccacgat gcgcgccatc tccggcatga
     5041 tcgcgccgcg cgagggcaag gtcatgctcg gcgacgtgga catcaccggc cgcgactcgc
     5101 atcgcatcgc gcgcctgggc ctggcgcact cgcccgaggg acggcgcgtg ttcgccacga
     5161 tgtcggtcaa cgacaacctg ctgctgggcg cattcccgcg ctacaccggc gcgcgcgcca
     5221 agggcgacat cgccgccgac ctcgagcgcg cgatggagta cttcccgcgc ctgaaggagc
     5281 gccgcacgca gctggcgggg acgctgtcgg gcggcgagca gcagatgctc gcgatggcgc
     5341 gcgcgatgat gatgaacccc gacgtggtgc tgctggacga gccgtcgatg ggcctcgcgc
     5401 cgatcctggt cgacgaggtg ttccgcatca tctcgcggct gcgcgccgag ggcatcacga
     5461 tgctgctcgt cgagcagttc gccgccgccg cgctcaacgt ggccgactac ggctacgtgc
     5521 tggagaacgg ccgcatcgcg gtgcacgggc ccgcggagaa gctcaggaac gacccggcgg
     5581 tgcaggcggc ctacctgggc ggcggccact gaaggcgccg ccgccgcggc tttagtccgc
     5641 gggaccgtcc gtgccggtgc ccgcgcttgc cgcgcctccc agccgcgtga gcgtggcccg
     5701 ggccttgcgc tgcgcgaagc gccaggtgtg gcgaccttcg cgcttggcct cgtacaacgc
     5761 gacgtcggcg gacttgtaga gctcgtcctg cgtcgtgccg tccgacggga acagcgccac
     5821 gccgatgctg gggctcgtgc gcatgtcgtg gccggcgtgc gggatcggcg cgtcgaaggc
     5881 ctcgcagatg cggtggcaga cgatgtcgac ggcgctgggc acgccggtgt cggccagcag
     5941 gatcgcgaac tcgtcgccgc ccaggcgcgc gaccacgtcg gccttgcgcg cgatgccgcg
     6001 caggcggcgg gcggtctcca ccagcagcgc gtcgccggcg tcgtggccca gcgtgtcgtt
     6061 gacctgcttg aagtggtcca ggtcgatcag cagcagcgcg aagcccgagt cctggcgctc
     6121 ggccgcggcg atcatctggc ggcagtagtc gccgaacatg cggcggttcg gcaggccggt
     6181 gagcgcgtcg ctgtaggcca gttcctcgag ctggtgctgc agcttggtga gctcctgcgt
     6241 gcgctgctcc accagccgcg ccagctcctt gctgcgctgc tgcacgtagc gcacgcgcac
     6301 ggcggcgacc gcggcgatca ccagcagcac ggcgatgatc ttgagcaggt cgaaccacca
     6361 cgtctggtac caggccggca ggatctcgac gtcgacctcc agcgggcgct ccgtccacgc
     6421 gccgtcgcgg ctggcggcct tcatgtgcag cacgtagcgg ccgggcgaca ggttggtgta
     6481 gccggccgag cggtgcgtgg tgtcgacctc gcgccaggtc ttgtcgtagc cctcgagcca
     6541 gtaggcgtag cggttgcgct cgggcgacgt gaagtccagc agcgagaact ccaccgagaa
     6601 gccgcccgct tccggcggga cggtcagctg cttgcggccg ctcgcgtcgg cgagcgcggc
     6661 cggcttgccg cccatctcga cgtcggtcac caccagcggc gccgagtaca cgcggtgcgt
     6721 caggcgctcg gggcggatga cggtcagtcc gcccaggccg ccgaagaaca gctcgccggt
     6781 ggggccgacg gcgccggagt tgacccagta cgtggagatc gccgcgccgt cggccggatg
     6841 cagcacgcgc agcgcgtagg tggacgggtc gaccacggcc acgccgtcgt cggtgctgat
     6901 ccagacgcgg ccgatcgcgt ccatcagcat cgtgtcgatg ttctcgctgg tcaggccgtc
     6961 gtccttgccg atgtggtgga acaccgcatg gccgccggcg tcgaacttct ccaggatctg
     7021 cacgcccgcg ttgttgccca cccagagccg gccttggcgg tcggtcagca gcgtgttgat
     7081 gacggccggt gacaggccgg tcgggtcgtt ggccgacggc atgatgcgct gcaccggctg
     7141 gccgggccgc agcaggttca ggccgttgtt cgtggcgatc cacatcgcgc cgtcggggcc
     7201 gtggacgatc gcgttgacct cggggtcggt caacgcgctc gtgggcacgg gctggcgcat
     7261 cgcgtgcgtg ggcgagtcca ggtccagctg ccagatgccg tcgcccttgc cgcccaccca
     7321 cagggtcgag ccgtccacct ccagcgcgat gacgcccttg acggcgccgc gctcgggcat
     7381 ggcgatggtc tcgagcttgc gggcgccggg cgccagccgg aacaggccgt cgcgcgacgc
     7441 catccagcgc gtgccgtcgg cggtgtgcac cacggccgtg gcgttgctga tcgcgggcgg
     7501 atgcgccggg tcgtcctcgc tgccgcccag cagcgcggtc atcttctgct cgatcgggtc
     7561 gatcacctcg atgccgttct tctggcccag cgtgacgacg ccgcgcgagt cgacgtccac
     7621 cgagaaaacg tacgggtcgg tccaggcgga gccgtcggac gagaacagcg acaggatcgc
     7681 gtcctgctgc gggcgcgtgc ggctcaggcc ggtggtggag gccacccaca tctcgccgcc
     7741 gcggtcgcgg tgcaggcccc agatccagtt cgcctgcagg ttgctcggca cgctgcggtc
     7801 gcggatgacg cggcgggcct gcagcgaatc ggtgtcggcg atcagcaggc cgcgcgtgga
     7861 cgtgccgatc cagatctcgc cgttgccgtt ctcctcgaac gacgtggcgc tgtcggtcag
     7921 caggccaggg tcggacgcgc cggccggctg caggcgcgag acgcggccgc tggccgggtc
     7981 gagcacgagc acgccgtgca ggttggtgcc gatccagatc tggccgtggg cgtcctcgta
     8041 gagcgagtcg accggcacgg cgccgccgtc ggcaccgccg gac
//
LOCUS       DATCRO010000027         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_664084, whole genome shotgun sequence.
ACCESSION   DATCRO010000027 DATCRO010000000
VERSION     DATCRO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664084"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..310)
                     /locus_tag="VIN75_00680"
     CDS             complement(92..310)
                     /locus_tag="VIN75_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972700.1"
                     /translation="MQASTRSNPFVMMTDPETILQAVERSERLNRLSRKVYRPLDRPL
                     IPKVGAAKELLDFDSAIDSAIEDDSLEA"
     gene            598..792
                     /locus_tag="VIN75_00685"
                     /pseudo
     CDS             598..792
                     /locus_tag="VIN75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            905..1156
                     /locus_tag="VIN75_00690"
     CDS             905..1156
                     /locus_tag="VIN75_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8972701.1"
                     /translation="MRRDRHGRGYASEAAFAMGDWAFDTFKPDELLAVCEPANTASSN
                     VMKRLGMHDRGLQRWYGKEMATFGIAADEWRANAAARKA"
     gene            complement(1171..1821)
                     /locus_tag="VIN75_00695"
     CDS             complement(1171..1821)
                     /locus_tag="VIN75_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8972702.1"
                     /translation="MSSRLRATAFAVAAFLSAGPAPAASYDSVDFFRAVNVDNADAVA
                     RMLAAGLDPNQVDPRGQPALILALQGESLKVAKVLWDAKGVDIDVRNRVGETPLMMAA
                     LKAEVDAASALVAHGAAVQKDGWSPLHYAATGGSAAIVRMLLAKGAVLEARSPNGTTP
                     LMMAARYGNEEAVDALLAAGADRTLKNDLGMDASAFAASAGREKLAARLKPATASK"
     gene            complement(1867..2667)
                     /locus_tag="VIN75_00700"
     CDS             complement(1867..2667)
                     /locus_tag="VIN75_00700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8972703.1"
                     /translation="MYVDSHCHLAFPELRERLGAILEDMRAADVGGALCICTSMDEFE
                     SVHALALAHDNLWASVGVHPDTEAMAEPDVEWLCERAARPRVIAIGETGLDYYRLEGR
                     SIADMEWQRERFRVHIRAARKLDLPLVIHTRSASDDTLAVLHAESEGAGPAPRGVFHC
                     FTESMDVARAALDLGFHISFSGILTFKTAADLREVARYVPLDRCLIETDSPYLAPVPH
                     RGKTNTPAWVPHVAHLIAELKGVPCEQVADATRANFEQLFRVNMAAPA"
     gene            complement(2720..3082)
                     /locus_tag="VIN75_00705"
     CDS             complement(2720..3082)
                     /locus_tag="VIN75_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8972704.1"
                     /translation="MGSPPPATARPSVIQLVFREKGALYAAYMPIFAEGGLFVPTTRD
                     YQLGDDIYLLLTLPGDAQRYPVAGKVGWITPANAPGGRTQGVGVRFPVDEKTRMLKVR
                     IEEVLGTSLSSSKPTQTI"
     gene            complement(3291..>4034)
                     /locus_tag="VIN75_00710"
     CDS             complement(3291..>4034)
                     /locus_tag="VIN75_00710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8972705.1"
                     /translation="DRAWVVDFCVKQSQSGMDHEADYRALGAAGQYVWIRDVVHVQRN
                     EAGEPLALIGFMFDISERKKTEEKLLVMRKQLEDYSFRDGLTGVSNRRKFDAVLEEEW
                     TLARRNTQPLSLILIDIDFFKQYNDHHGHVQGDECLKAVAAALGTVANRPRDLLARYG
                     GEEFVLVLPATDESAAQRVSERVRQALFKAQIPHAASSVGQLLTVSQGVGTIIPSSTD
                     TLVQFLDTVDRRLYRAKQAGRDRSVAGAA"
BASE COUNT          582 a         1399 c         1438 g          615 t
ORIGIN      
        1 ggagaaatca ggtcgccgcg cggtgctcat cgatcgatcg aggagcggcg gcgacgccgt
       61 ccttcgaagg ccgcgacggc cttcggcccg ctcaggcttc gagggaatcg tcctcgatgg
      121 ccgagtcgat ggcgctgtcg aagtccagca gttccttggc ggcgccgacc ttggggatca
      181 gcgggcgatc cagcggacgg tagaccttgc ggctcaggcg attcaggcgc tccgagcgct
      241 cgacggcctg caggatggtc tccggatcgg tcatcatcac gaacgggttc gaacgggtcg
      301 aggcttgcat gaaaagtctc ctggtgttgc gtggagatgg ccgcgaagca gccgtctgtc
      361 catggagcga actgtagaac ccgcctccgc gaatctgtag tcgtctctgc gccagaccgc
      421 gtgtctccga acttcctaca aaatgagggg aacactgggt aaagcgcgac gcttttcacg
      481 ctttcgcgcg aggggacctg cgtcaggaga tattgatgcc gcggcctgcc gccccgcgcc
      541 ggcggtccgc gccgcgcggt cgatacttcc ccactcgccc gaaccacgac gtccacgatg
      601 accatcctca cgaccgcccg cctgcgcctc gacccctgcg cgccggcgca cctggacggc
      661 ctgcacgcgc tcaacgccga ccccgaggtc atgcgctaca tctccggcaa gcccgagacg
      721 cgcgaggaga cgcaggccat gatcgcccgc gtccaggcgc gctgggccga gttcggctac
      781 tcgtggtgga cgttcatcga gcgcgacggc ggccagatcg tcggcgcggg cgccctgcag
      841 aacctgcgcc gcgagatgac gccccacgcc gacccggcct gcccggctgg aactgggctg
      901 gcgcctgcgc cgcgaccgcc acggccgcgg ctacgccagc gaggctgcct tcgcgatggg
      961 cgactgggcg ttcgacacct tcaagcccga cgaactgctg gccgtctgcg agccggccaa
     1021 cacggcgtcg tccaacgtga tgaagcgcct gggcatgcac gaccgcggtc tgcagcgctg
     1081 gtatggcaag gagatggcga cgttcgggat cgcggccgac gaatggcggg cgaacgccgc
     1141 ggcgcgcaag gcgtagggcg gaacggccgg ctacttcgaa gccgtcgccg gcttcagtcg
     1201 cgccgccagc ttctcgcgcc ccgcgctcgc cgcgaacgcc gacgcatcca tgcccaggtc
     1261 gttcttgagc gtgcggtccg cgcccgccgc cagcagcgcg tcgaccgcct cctcgttgcc
     1321 gtagcgcgcg gccatcatca gcggcgtggt gccgttgggc gagcgcgcct cgagcaccgc
     1381 acccttggcc agcagcatcc tgacgatcgc cgcgctgccg ccggtggcgg cgtagtgcag
     1441 cggcgaccag ccgtccttct gcaccgcggc gccgtgggcg acgagcgcgc tggcggcgtc
     1501 gacctcggcc ttgagcgcgg ccatcatcag cggcgtctcg ccgacgcggt tgcgcacgtc
     1561 gatgtcgacg cccttggcgt cccacagcac cttggccacc ttcagcgatt cgccctgcag
     1621 cgcgaggatc agcgcgggct ggccgcgcgg gtcgacctgg ttcggatcca gccccgcggc
     1681 gagcatgcgc gcgacggcgt cggcgttgtc gacgttgacg gcacggaaga agtcgacgct
     1741 gtcgtagctg gcggccggcg ccgggccggc ggacaggaag gcggcgacgg cgaacgccgt
     1801 ggcgcgaagg cgggaagaca tggtgcggac tcttgcaggt gacgtgcgcg acgaaaccgg
     1861 actggatcag gctggcgccg ccatgttcac acggaaaagt tgctcgaagt ttgcgcgcgt
     1921 ggcgtcggcc acctgctcgc acgggacacc cttgagctcg gcgatcaggt gggcgacgtg
     1981 cggcacccag gccggggtgt tggtcttgcc gcggtgcggc accggcgcca ggtaggggct
     2041 gtccgtctcg atcaggcagc ggtccagcgg cacgtagcgc gcgacctcgc gcaggtcggc
     2101 ggccgtcttg aaggtgagga tgcccgagaa cgagatgtgg aagccgaggt cgagcgccgc
     2161 gcgggcgacg tccatcgact cggtgaagca gtggaacacg ccgcgcggcg cggggccggc
     2221 gccttcggat tcggcgtgca gcacggccag cgtgtcgtcg ctggccgagc gcgtgtggat
     2281 caccagcggc aggtcgagct tgcgagcggc acggatgtgg acgcggaagc gctcgcgctg
     2341 ccactccatg tcggcgatcg aacggccctc gaggcggtag tagtccagcc ccgtctcgcc
     2401 gatcgcgatg acgcgcggcc gggcggcgcg ctcgcacagc cactcgacgt cgggctcggc
     2461 catcgcctcg gtgtccggat gcaccccgac gctggcccac aggttgtcgt gggccagggc
     2521 cagcgcgtgc acggactcga actcgtccat gctggtgcag atgcacagcg cgccgccgac
     2581 gtcggcggcc cgcatgtcct ccaggatcgc gccaaggcgc tcgcgcagtt cggggaacgc
     2641 gaggtggcag tgggagtcga cgtacatggg aagtcagggt cgcgccgggc gcgcgtgacg
     2701 cgtccgcgtg cgcaaccgat cagatcgttt gagtcggctt ggacgacgac agcgaggtgc
     2761 ccagcacctc ctcgatgcgc accttgagca tgcgcgtctt ctcatccacc gggaagcgga
     2821 cgccgacgcc ctgcgtgcgg ccgcccggcg cgttggcggg cgtgatccag ccgaccttgc
     2881 cggccaccgg atagcgctgc gcgtcgccag gcagcgtcag cagcaggtag atgtcgtcgc
     2941 ccagctggta gtcgcgcgtg gtcggcacga acaggccgcc ctcggcgaag atgggcatgt
     3001 aggcggcgta cagcgcgccc ttctcgcgaa agacgagctg gatgacgctc ggccgcgccg
     3061 tcgccggcgg cggggagccc atcacggaat tcggttcatc tgccatcgtc ccgccagtgt
     3121 agcgagcttt cccgcccctc gatggctcgg cagcgatcgg ttaccggtgg cttattgcac
     3181 gttcggcgtc gcgttcggcg cgtccggcca gggcgctcgc tgcgctggcc ggccggcgcg
     3241 gacggggtgc cgcgcctcgg cggcctggtc cgtcccgcct gcgatgcctg tcaggccgcg
     3301 cccgcgacgc tgcggtcgcg ccccgcctgc ttggcgcggt acaggcggcg gtcgacggtg
     3361 tcgaggaact gcaccagcgt gtcggtggac gacgggatga tggtgcccac gccctggctg
     3421 acggtcagca gctggcccac cgacgacgcc gcgtgcggga tctgcgcctt gaacagcgcc
     3481 tggcgcacgc gttcgctgac gcgctgcgcg gcgctctcgt cggtggcggg cagcaccagc
     3541 acgaactcct cgccgccgta gcgcgccagc aggtcgcgcg ggcggttggc caccgtgccg
     3601 agcgccgccg ccacggcctt gaggcactcg tcgccctgca cgtggccgtg gtggtcgttg
     3661 tactgcttga agaaatcgat gtcgatcagg atcagcgaca gcggctgcgt gttgcggcgc
     3721 gccagcgtcc attcctcctc gagcaccgcg tcgaacttgc ggcgattgga cacgcccgtc
     3781 agcccgtcgc ggaacgagta gtcctcgagc tgctttcgca tcaccaacag cttttcctcg
     3841 gtcttcttgc gctcgctgat gtcgaacatg aagccgatca gcgccagcgg ctcgccggcc
     3901 tcgttgcgct gcacgtgcac gacgtcgcgg atccacacgt attgccccgc ggcgccgagc
     3961 gcgcggtagt cggcctcgtg gtccatgccg gactgcgact gcttcacgca gaagtccacc
     4021 acccaggcgc gatc
//
LOCUS       DATCRO010000028         8116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_585289, whole genome shotgun sequence.
ACCESSION   DATCRO010000028 DATCRO010000000
VERSION     DATCRO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585289"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..314
                     /locus_tag="VIN75_00715"
     CDS             39..314
                     /locus_tag="VIN75_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY8972706.1"
                     /translation="MPRYLVERTFPEGLHIPVNNQGADVCMTVVASNARSGVTWVHSY
                     VSDDQKKTFCIYDAPNPEAIRETARSNNLPIDSITAVSVLDPYFYRG"
     gene            complement(302..1486)
                     /locus_tag="VIN75_00720"
     CDS             complement(302..1486)
                     /locus_tag="VIN75_00720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8972707.1"
                     /translation="MTIVDRWKRWLAKGEPQPATALLPSHSFEDDVTGLVNRRVMHDT
                     LVAALQDDVPGAVVTFDLDNFKYFNDRHGHVVGDDLLRLVAARLREALPGAGCLARVG
                     GDQFMAFLPERDLAGALRAAQACVDRMREPVILSASAEIVTLSAGVATLDAQSARAVD
                     AVWRACDAALHAAKARGRDRVVAFDDDTRRIVASRRDLLSTVAELQERNRALREEART
                     DALTGLRNRLALDELLHAVAGDGDRRVQRAAVAFIDVDHFGNFNHTQGDAAGDEALRT
                     VAHAIRAVAREADLVFRKGGEEFVVVLPDADGHAASAAAERIRGAVEALALAHTASPV
                     AGVVTVTIGVASGPSGVTLRQLLEAAAERTMAAKVGELRNRVHAVRLPDVEGPVEASQ
                     PR"
     regulatory      complement(1684..1766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1862..2329
                     /locus_tag="VIN75_00725"
     CDS             1862..2329
                     /locus_tag="VIN75_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY8972708.1"
                     /translation="MTSCRPATRFLAAALLALAADLAGAAGPAPAAPAAAAAPADGKE
                     HAIFAVSDDDTKRWNLTLGNIGNAIDGLGAESADIELVVYGPGIAMLRKDSPVADRIA
                     AALKRGVHVYACQNSMRGYHLEQADLAPGVGTVPSGVVELIRRQHAGYAYVRS"
     gene            complement(2336..2698)
                     /locus_tag="VIN75_00730"
     CDS             complement(2336..2698)
                     /locus_tag="VIN75_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY8972709.1"
                     /translation="MRIVLLTSMLALATAAGAAPLRDPAQPPSAVRQAAQAQNRMRPA
                     SVGATLDAEAESHLRASFDAADVAHRGTLTREEARAGGFGWIANHFDAIDTAHAGRVS
                     FDDVRRYLKARDANAATR"
     gene            complement(2698..4260)
                     /locus_tag="VIN75_00735"
     CDS             complement(2698..4260)
                     /locus_tag="VIN75_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY8972710.1"
                     /translation="MALFQTVLRTARSAAPAALLAVVAACGGGGGGDNIGGSGANGSQ
                     YIDANHYSAAAGASLSTPSEATGVTHGSLTLGAGTIAYTTTAGHLSALDANQTPEASI
                     FYVAYTADGAAAATRPVTFFFNGGPGSASIWLHLGSFAPRRLSTGVPATTASVPFPYV
                     DNAESLIDASDLVFVDAVGTGLSEAIAPRTNQDFWGVDKDAAVLRDFIQRWLAANGRT
                     ASPLFLFGESYGTTRVPVLARLLETAGQRVHGVVELSSIVDYNSNCSVAGGTPTVNCA
                     GFLPSYAAVGDWWHVAPGGTSDLDADIAAVRSYADSTYRPDADAWLASGTLPPADHIS
                     ALVSFTGLGADIWQQRFDMDFDTFRHHLIANTTLGVYDGRMSALVGSPLDANDDPSNT
                     FVEPGFESEIRTLLPALGYTNPSTYVLSSNAIATWDFSHAGRALPDVVPDLSAALALD
                     PQMKVLFLGGEHDLITPFHQTELDVARLGAGAPVTLHFHPGGHMTYLDDGSRPLMKAE
                     LAAFYASATGAQ"
     gene            4473..5162
                     /locus_tag="VIN75_00740"
     CDS             4473..5162
                     /locus_tag="VIN75_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972711.1"
                     /translation="MTHPTARPRLAALALSTLALAAARPAAAVEAYAATGFPYALAGV
                     AQPINGSFAVRADFGGIAHHAYSGTTSDNDFKGNIDYNRFALLGDWFVASGGFRLTAG
                     ATVNTAKATMKASSHNGTITIGGTQYNAPSSTYYVTSELSFPKTTPYVGLGWGHHDSA
                     PGLSFSFDVGASIGTAKATPLQASPALASELALNQQGAADLAQENRDFQDEVHKFKAI
                     PQLTVGLGYRF"
     gene            complement(5150..5539)
                     /locus_tag="VIN75_00745"
     CDS             complement(5150..5539)
                     /locus_tag="VIN75_00745"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEY8972712.1"
                     /translation="MHIALIAHDAKKDLMVALARDFAPFLSRCHVMATGTTGSRLIVE
                     AGIEVERLLSGPLGGDLQIGARLAEGLVDAVIFLRDPMTPQPHEPDINALVRACDVHD
                     VVCATNESTARHVLRALSEELDAAQKR"
     gene            complement(5745..6032)
                     /locus_tag="VIN75_00750"
     CDS             complement(5745..6032)
                     /locus_tag="VIN75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY8972713.1"
                     /translation="MTSQHDQSGREKQQSQAGATGQQGGQTSGGRGFAGMDRERQREI
                     ASQGGRAAHASGNAHQFTSEEARAAGSKRHANAAMRAAQAAQANKGDSSVN"
     gene            complement(6302..7177)
                     /locus_tag="VIN75_00755"
     CDS             complement(6302..7177)
                     /locus_tag="VIN75_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8972714.1"
                     /translation="MTPPASSAPADPARRRLLAAGALLALPGAAALAQPREGVQVGPR
                     SGVESLVPAAQLEAEAARGYHEMIAQARAAHRLAPPEHVQLRRLVMISSRLRPFAGMW
                     NPRAAGWQWEVNLINSPQVNAFCMPGGKIAFFWGILAKLQLTDDEVAMVMGHEMTHAL
                     REHGRSQIGKQVATQGAIALLAGLFGWSNAGRQAANVGGHLLSLKYSRDDETEADLIG
                     LELAARAGYDPRSALSLWHKMEQASRGAPPAFLSDHPSNPDRLRTIAANIPRVQGLFE
                     RAPKPDQRFGPPLAG"
     gene            complement(7231..>8116)
                     /locus_tag="VIN75_00760"
     CDS             complement(7231..>8116)
                     /locus_tag="VIN75_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8972715.1"
                     /translation="VERVDRPPQQLQQDQRGHAHRHGDQVPKGSLQAKWIDLLALLLG
                     LAATLPLAGFAARRAKFQTLLAGLDSYFSQPRAWSFLAFIVFYKLTDAFQLSLLTPFL
                     LKGMAFGPAEVGVVNKLIGLLLTVLGALLGGLVMLRLRLVWSLLVFGLLQMSSSLAFW
                     WLAVHGKGVLPGWTIPAFDFGFVKLLHPTQVDGGLLMAVGAENLASGMGTAAFLAFLM
                     SLTNSRFSATQFALLSAFQSVGRVWIGPLAGVLAEAIGWPSFFLVAVAMGVPSLILLK
                     LLWRPIDALDARGARPAP"
BASE COUNT         1198 a         2894 c         2915 g         1109 t
ORIGIN      
        1 ggtcgaacgc atcgactgca accaaccagg agtccgccat gccccgctat ctcgtcgaac
       61 gcacgttccc cgaaggcctg cacatccccg tgaacaacca gggcgccgac gtctgcatga
      121 cggtcgtcgc gagcaacgcc aggagcggcg tgacctgggt gcactcctac gtcagcgacg
      181 accagaagaa gacgttctgc atctacgacg ccccgaaccc cgaggccatc cgcgagaccg
      241 cgcgcagcaa caacctgccg atcgactcga tcaccgcggt gagcgtgctg gatccgtact
      301 tctatcgcgg ctgagaggcc tcgaccggcc cctcgacgtc gggcagccgc accgcgtgca
      361 cgcggttgcg cagctcgccc accttggccg ccatcgtccg ttcggcggcg gcctccagga
      421 gctggcgcag ggtgacgccg gacgggccgc tggccacgcc gatggtcacc gtgacgacgc
      481 cggccacggg cgaggccgtg tgcgcgagcg cgagcgcctc gacggcgccg cggatgcgct
      541 ccgcggcggc cgacgcggcg tggccgtcgg cgtcgggcag caccaccacg aactcctcgc
      601 cgcccttgcg gaacaccagg tcggcctcgc gcgccacggc gcggatggcg tgcgccaccg
      661 tgcgcagcgc ttcgtcgccg gcggcgtcgc cctgggtgtg gttgaagttg ccgaagtggt
      721 cgacgtcgat gaaggcgacc gcggcgcgct ggacgcggcg atcgccgtcg ccggccaccg
      781 catgcagcag ctcgtcgagc gccagacggt tgcgcaggcc ggtgagcgcg tcggtgcgcg
      841 cctcctcgcg cagcgcgcgg ttgcgctcct gcagctcggc caccgtggac agcaggtcgc
      901 gccgcgacgc gacgatgcgg cgcgtgtcgt cgtcgaacgc gacgacgcgg tcgcgcccgc
      961 gcgccttggc cgcgtgcagc gcggcgtcgc aggcgcgcca gacggcgtcc acggcgcgcg
     1021 cggattgcgc gtcgagcgtg gccacgccgg ccgacaaggt gacgatctcg gcgctggccg
     1081 acaggatgac gggctcgcgc atccgatcga cgcaggcctg cgccgcgcgc agcgcgccgg
     1141 ccaggtcgcg ctcgggcagg aacgccatga actggtcgcc gcccacgcgc gccaggcagc
     1201 cggcgccggg cagcgcctcg cgcaggcgcg cggccaccag gcgcagcagg tcgtcgccca
     1261 cgacgtggcc gtggcggtcg ttgaagtact tgaagttgtc gaggtcgaac gtgacgaccg
     1321 cgccgggcac gtcgtcctgc agcgcggcca ccagcgtatc gtgcatgacg cgccggttca
     1381 ccaggcccgt gacgtcgtcc tcgaacgaat gcgacggcag caaggccgtc gccggctgcg
     1441 gctcgccctt ggccagccag cgcttccagc gatcgacgat cgtcatggcc gccccccgcg
     1501 ccggcgggca cgcgcccgtg cccgcacgct cacgcgcgcg acccgtccga ttggcatgtt
     1561 gtgcatggcc gccatctccc tgtcactcac gcggacacgc gcatggttcg ggatcgcgac
     1621 cggcgagcct ccgcactccg gcggttcggt ggctcacgac gtccgggcgc gacgaccgcg
     1681 cccggtggca gccccgctgc cctccgctct tggagggtcg gtggctttgc gaccccggct
     1741 ctcgcacggg tgtgcccttg actgaacgat attacagcga agttaggggg aagtgagaag
     1801 tcctgtcttg cgaagacggc aaactgaggg tatgtccgac cgatttccca ccggagagcc
     1861 catgacgtcc tgccgccccg ccacccgttt cctggccgcc gccctgctcg cgctggccgc
     1921 cgacctcgcc ggcgccgccg gccccgcgcc cgccgcgccg gccgccgccg cggcccccgc
     1981 cgacggcaag gagcacgcga tcttcgcggt cagcgacgac gacacgaaga gatggaacct
     2041 gacgctgggc aacatcggca atgccatcga cggcctgggc gccgagtcgg ccgacatcga
     2101 gctggtggtc tacggcccgg gcatcgcgat gctcaggaag gactcgccgg tggccgacag
     2161 gatcgcggcg gcgctcaaga ggggcgtgca cgtctatgcg tgccagaact cgatgcgcgg
     2221 ctaccacctg gagcaggccg acctcgctcc gggcgtgggc accgtgccgt ccggcgtggt
     2281 cgaactgatc cggcgccagc acgccggcta cgcctacgtg cggtcctgag acgggtcagc
     2341 gcgtggcggc gttcgcgtcg cgcgccttca ggtaacgccg gacgtcgtcg aagctgacgc
     2401 ggccggcgtg ggcggtgtcg atggcatcga agtggttggc gatccagccg aagccgccgg
     2461 cgcgggcctc ctcgcgcgtg agcgtgccgc ggtgggcgac atcggccgca tcgaagctgg
     2521 cgcgcaggtg cgattccgcc tcggcgtcca gcgtggcgcc gaccgaggcc gggcgcatgc
     2581 ggttctgggc ctgggcggcc tggcgcacgg ccgacggcgg ctgggcgggg tcgcgcaggg
     2641 gggcggcgcc cgcggcggtg gccagcgcca gcatcgaggt gagcaggacg atgcgcatca
     2701 ttgggctccc gtcgccgagg cgtagaacgc ggccagttcg gccttcatca gcgggcgcga
     2761 gccgtcgtcg aggtaggtca tgtggccgcc cggatggaag tgcagcgtca ccggggcgcc
     2821 cgcgcccagg cgcgcgacgt ccagctccgt ctggtggaac ggcgtgatca ggtcgtgctc
     2881 gccgccgagg aacagcacct tcatctgcgg atcgagggcc agcgcggcgc tgaggtcggg
     2941 cacgacgtcg ggcagggcgc ggcccgcgtg cgagaagtcc caggtggcga tcgcgttgct
     3001 ggacagcacg taggtgctcg ggttcgtgta cccgagcgcg ggcagcagcg tgcggatctc
     3061 cgactcgaag cccggctcga cgaaggtgtt ggacgggtcg tcgttcgcgt ccagcggcga
     3121 gccgaccagc gcggacatgc ggccgtcgta cacgcccagc gtcgtgttgg cgatcaggtg
     3181 gtggcggaac gtgtcgaagt ccatgtcgaa gcgctgctgc cagatgtccg cgcccaggcc
     3241 ggtgaaggac accagcgcgc tgatgtggtc ggccggcggc agcgtgccgc tggccagcca
     3301 cgcatcggcg tcgggccggt aggtggaatc ggcgtagctg cgcacggcgg cgatgtcggc
     3361 gtcgaggtcg gacgtgccgc ccggggccac gtgccaccag tcgcccaccg cggcgtagct
     3421 cgggaggaag ccggcgcagt tgacggtcgg cgtgccgccc gcgacagagc agttgctgtt
     3481 gtagtcgacg atcgacgaca gctccacgac gccgtgcacg cgctgcccgg cggtctcgag
     3541 caggcgcgcg agcacgggca cgcgcgtggt gccataggac tcgccgaaca agaacagcgg
     3601 cgaggcggtg cggccgttgg cggcgagcca gcgctggatg aagtcgcgca gcacggcggc
     3661 gtccttgtcg acgccccaga agtcctggtt ggtcctgggc gcgatcgcct cggacaggcc
     3721 cgtccccacg gcgtcgacga agacgaggtc gctggcgtcg atcaggctct cggcgttgtc
     3781 gacgtacggg aacggcacgc tcgcggtcgt ggccgggacg cccgtggaca ggcgccgcgg
     3841 cgcgaacgag cccaggtgca gccagatgct ggccgagccc gggccgccgt tgaagaagaa
     3901 cgtgacgggc cgggtcgccg ccgcggcgcc gtcggccgtg taggcgacgt agaagatcga
     3961 cgcctcgggc gtctggttcg catcgagcgc gctcaggtgg cccgcggtgg tggtgtaggc
     4021 gatcgtgccg gcgcccagcg tcagcgagcc gtgcgtgacg ccggtggcct cgctgggcgt
     4081 ggaaagcgac gctccggccg ccgcggagta gtggttcgcg tcgatgtact gagagccgtt
     4141 ggcgccgctg ccgccgatgt tgtcgccgcc tccaccgccg ccgcaggccg ccacgacggc
     4201 cagcagggcc gctggcgcgg cggaccgcgc ggtgcgcagg acggtctgga acagggccat
     4261 cgattcctcc cggctgatgc cgatgtacgc ccggacactg cggttgccat gcgaaaccgg
     4321 cgggcaggcg gtttcgctgc gacgattgtg gcccaagctc cggggccgct cccatcagcc
     4381 cggaaaggcg acgtccggcc cggcttttca ggcgtccggc gcggcccgat ccgccgtacg
     4441 ctggcggctt ccctccaacg acaaggttcc cgatgaccca ccccaccgcc cgaccccgcc
     4501 tcgccgccct ggcgctctcg accctggccc tggccgccgc gcgacccgcc gccgcggtcg
     4561 aggcgtatgc cgccaccggc ttcccgtatg cgctggcggg cgtcgcccag ccgatcaacg
     4621 gctcgttcgc ggtgcgcgcc gacttcggcg gcatcgccca ccacgcctac agcggcacga
     4681 cgtccgacaa cgacttcaag ggcaacatcg actacaaccg cttcgcgctg ctgggcgact
     4741 ggttcgtggc cagcggcggc ttccgcctca ccgccggcgc cacggtcaac acggccaagg
     4801 ccacgatgaa ggcgtcgtcc cacaacggca cgatcaccat cggcggcacg cagtacaacg
     4861 cgccttcgtc gacctactac gtgacgtccg agctctcgtt ccccaagacc acgccctacg
     4921 tgggcctggg ctggggccac cacgacagcg cgcccggtct gtcgttcagc ttcgatgtcg
     4981 gcgcgtccat cggcaccgcc aaggccacgc cgctgcaggc gtcgccggcg ctcgccagcg
     5041 aactcgcgct caaccagcag ggcgccgccg acctcgcgca ggagaaccgc gacttccagg
     5101 acgaggtcca caagttcaag gcgatcccgc agctgacggt gggcctgggc taccgcttct
     5161 gagcggcgtc gagttcttcc gaaagtgcgc gcagcacatg gcgcgcggtc gactcgttgg
     5221 tggcgcagac gacgtcatgc acgtcgcagg cgcgcaccag cgcgttgatg tccggctcgt
     5281 gcggctgcgg ggtcatcggg tcgcgcagga agatcaccgc gtcgacgagt ccctcggcca
     5341 ggcgcgcgcc gatctgcagg tcgccgccca gcgggccgct gaggaggcgt tcgacctcga
     5401 tgcccgcctc gacgatcagg cgcgagccgg tggtgccggt ggccatcacg tggcagcggg
     5461 agaggaacgg ggcgaagtcg cgcgcgaggg cgaccatcag gtccttcttg gcgtcgtgcg
     5521 cgatcagggc gatgtgcatc acccgatcat aagcacggtc ggttacgacg tccgatcaat
     5581 cggcaggccc caggatgggg catcggcttt acgaaacttc gcagagccgc ggcggccagg
     5641 cgccccccca aaaaagcaaa gcgcgggacg agcccacgct ttgctgtagc gtttgcagca
     5701 aaccgggtcg gccggcccgc cctggaagcg cgccgacgaa ccagtcagtt gaccgacgaa
     5761 tcgcccttgt tcgcctgggc ggcttgcgcg gcgcgcatcg cggcgttcgc gtggcgcttg
     5821 gagccggcgg cccgcgcttc ttccgacgtg aactggtggg cgttgccgct ggcgtgcgcc
     5881 gcacggccac cctggctggc gatctcgcgc tggcgctcgc gatccatgcc ggcaaaaccg
     5941 cgtccgccgc tggtctggcc gccctgctgg ccggtggcgc cagcttggct ttgctgcttc
     6001 tcgcggccgg attggtcatg ttgggaagtc attggtgcta ctccgaaagg tttggtccgc
     6061 cgccccatgc gacaggattt atcggacccg cgagcgccag agcacccagg tcacggacga
     6121 catgggcaac gctcatacct cccccggcac ctccccccta atgccgagca ggaaacgtgg
     6181 cgaaacacgg cgtgcgcgcc gcttgggtgc acgcgtcgag acgatgcccg cccccccgtc
     6241 aagtccccca ccttcgatgc ggcgatccgc aatccctgcc ggaaaaatcg gcagtccatg
     6301 atcatccggc cagtggcgga ccgaaacgct ggtcaggctt cggggctcgt tcgaacaggc
     6361 cctgcacgcg cgggatgttg gccgcgatgg tgcgcaggcg atccgggttg gacggatggt
     6421 ccgacaggaa cgccggcggc gcgccccggc tcgcctgctc catcttgtgc catagcgaca
     6481 gtgccgatcg cgggtcgtag cctgcgcgcg cggcgagctc caggccgatc aggtcggcct
     6541 cggtctcgtc gtcgcggctg tacttgagcg acagcaggtg gcccccgacg ttggccgcct
     6601 ggcggccggc gttgctccag ccgaacaagc ccgcgagcag cgcgatcgcg ccctgcgtgg
     6661 cgacctgctt gccgatctgc gagcggccat gctcgcgcag cgcgtgggtc atctcgtggc
     6721 ccatgaccat cgccacctcg tcgtcggtga gctgcagctt ggcgaggatg ccccaaaaga
     6781 aggcgatctt gccccccggc atgcagaacg cgttgacctg cggcgagttg atcaggttga
     6841 cttcccactg ccagccggcg gcgcgcgggt tccacatgcc cgcgaacggc cgcaggcggc
     6901 tcgagatcat caccagccgg cgcagttgca cgtgctcggg cggcgccagg cgatgggcgg
     6961 cgcgggcctg cgcgatcatc tcgtggtacc cgcgcgcggc ctccgcctcg agctgcgcgg
     7021 cgggcaccag ggactcgacg ccggaacggg gcccgacctg cacgccctcg cgcggttgcg
     7081 ccagcgccgc ggcgccgggc agcgccagca gggcgccggc ggccagcagg cggcggcgcg
     7141 ccggatcggc gggggcggag gaggcaggcg gggtcacggg cgtcgtccgg ggaaatggcg
     7201 gatctgcact tgcatccgat ccaccgggtt tcaaggggcc gggcgcgccc cgcgcgcgtc
     7261 gagcgcgtcg atcggccgcc acagcagctt cagcaggatc agcgagggca cgcccatcgc
     7321 cacggcgacc aggaagaagc tcggccagcc gatggcctcg gccagcacgc ccgccagcgg
     7381 gccgatccac acccggccca ccgactggaa cgccgacagc agcgcgaact gcgtggcgct
     7441 gaagcggctg ttggtcaggc tcatgaggaa cgcgaggaag gccgcggtgc ccatgccgct
     7501 ggccaggttc tctgcgccca ccgccatcag caggcccccg tcgacctgcg tcggatgcag
     7561 cagcttgacg aagccgaagt cgaacgcggg gatcgtccag cccggcagca cgcccttgcc
     7621 gtggacggcc agccaccaga aggcgaggct gctcgacatc tgcagcagcc cgaacaccag
     7681 cagcgaccat acgagccgca gccgcagcat cacgagcccg cccagcagcg cgcccaacac
     7741 cgtcagcagc aggccgatca gcttgttgac gacgcccacc tccgccgggc cgaacgccat
     7801 gcccttgagc aggaagggcg tcagcagcga cagctggaac gcgtcggtga gcttgtagaa
     7861 gacgatgaac gcgaggaagc tccacgcgcg cggctggctg aagtacgagt ccaggcccgc
     7921 cagcagcgtc tggaatttcg cgcgccgcgc cgcgaagccg gccagcggca gcgtcgccgc
     7981 caggcccagc agcagcgcga gcaggtcgat ccacttggcc tgcagcgagc ccttgggcac
     8041 ctggtcgccg tggcgatggg cgtgccctcg ctgatcctgc tgaagctgct gtggcggccg
     8101 atcgacgcgc tcgacg
//
LOCUS       DATCRO010000029         7992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_438984, whole genome shotgun sequence.
ACCESSION   DATCRO010000029 DATCRO010000000
VERSION     DATCRO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438984"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VIN75_00765"
     CDS             complement(<1..273)
                     /locus_tag="VIN75_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972716.1"
                     /translation="MSDPNFPPSDDPDATMMIPKPGGRRAATPAPSAGQAMTLDTSAV
                     LSDVEDGGAPVGMNPLVANASVLLNTVPTIRRTMQHPDPAGLREYLL"
     gene            complement(290..1627)
                     /gene="tssK"
                     /locus_tag="VIN75_00770"
     CDS             complement(290..1627)
                     /gene="tssK"
                     /locus_tag="VIN75_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY8972717.1"
                     /translation="MSRKRVIWSEGLFLRPQHFQQLERSVEMLVESRATGVFAPGWGL
                     ANLRVDDEALKIGRLSIKSARGVMPDGTPFAIPEEYAAPLALEIGTETKDTVVHLALP
                     LVRAGMPEFAVDAGPATALARFVGQDLRVEDNVQGVGDPADMKLGQLNLRLMLDDEPR
                     SGFCSIAVARIIERRSTGQLVLDESFIPPALDCRGFARLQDYLVEARNLVRHRADTLA
                     ARLNQSQQKGVGEIGDFMMLQVANRYDPWLTHLSEREALHPEALYAALVQAAGEFATF
                     SDRRKRRARVQPIYRHEDLKNTFEPLMFDIRELLTEVINPNAVPIPLQERAAGLYTGM
                     VPDGDLLRNAQFVLAVNADKPGEFVRSRFPSFVKIGPPDKIRDLVRLHLPAIAVEPLP
                     MAPPRMPYYAGYTYFQLDRSGDLWKQLDVTKVIAMHIAGDFPGLQLELWALKP"
     gene            complement(1905..3338)
                     /locus_tag="VIN75_00775"
     CDS             complement(1905..3338)
                     /locus_tag="VIN75_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY8972718.1"
                     /translation="MPSSIELNIGRVPSPGRRDRAGDDAAGPFRIVVLGDFSGRPRDE
                     RAPLGERLPLRIDIDNFDAQLARVAPRAEIAGLQDKQGRPLRIEVETMDEFSADGVIA
                     RLPADALARAGTPPSPAPATSTGGDDAADLMKRLLGGDLPAGAPALAAAPASTSNRPD
                     AVVDRLIRQLIGDQASAPAPAPAAAAVTDAAAPMLRQVLRDPAWRRLECAWRAVDRFV
                     RTLDMDDGSVRLELFDCRADELLADLSACAGDPARSGLAPALKGDCRGCDVLVSLEEF
                     GPSVPELSLLGGLANVAAAWGAVLLAGAAPALAAVATSQDAQVLASNEARVWRALRES
                     AIAPHTGLTFPRLLARLPYGPRHDPVAAFAFDELADSTGASLHDRLAWRSAALDAALL
                     LAQGHTRGGVETEVQMEDLPAFIDRSGDEPRLQAVAEAYLGEGEAGVLQAAGLITLQS
                     DRRVPNARVTAWRSIAATGGELAGSWR"
     gene            complement(3364..3882)
                     /locus_tag="VIN75_00780"
     CDS             complement(3364..3882)
                     /locus_tag="VIN75_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEY8972719.1"
                     /translation="MPAAVQARLWQEAGDMPAYVILDGAQNPGLLDALYADHAPPWRC
                     LFGGQLEPDMATVAPYLVELEHSAAFTRRLFAEGWGQNWGVFLTSQLALPALWRHVRQ
                     QVHVYGPDMESLFFRFYDPRVMRNYLPTCPVAQLGEFFGPVDFYIAEGEVPTRAHAWS
                     VADGKLVAQSIG"
     gene            complement(3913..4302)
                     /locus_tag="VIN75_00785"
     CDS             complement(3913..4302)
                     /locus_tag="VIN75_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HEY8972720.1"
                     /translation="MPNHVCNGALLKCTMGMAPSSLVVLPLNMQNTSNMPAANIQDHI
                     PMVNIMPFGMCQSPSNPMVAAATAAAMGVLTPMPCIPVTPAPWVAGSPTVTLANQPAL
                     NKSSMLMCNWAGQISIQMEGQMTHTIP"
     gene            complement(4359..6425)
                     /gene="tssI"
                     /locus_tag="VIN75_00790"
     CDS             complement(4359..6425)
                     /gene="tssI"
                     /locus_tag="VIN75_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY8972721.1"
                     /translation="MKRMLEISTKVGADVFVVQRFSGREELGRLYEYQLDLVSERGDI
                     TSDQMLGSNATVALELMDGQASRYFNGYVTSFSVLGSVSTPAFKSNTGFLYKVTLHPG
                     VWFRTRSSNSQIFSTITIGDLIAQQLNVDTLMTIDNQVGSTEQRDFVVQYRETDFAFV
                     SRLAEHAGIYYYFTHDNGSHKMVLLNDAGSHKTTPGLSTVSFHGPADTDGTVTGFTLI
                     SQVQSGAFATGDYNYVTPNTQIAAVDSHPKSHDNAGFEMFDYPIDTGTAADATSYASI
                     RAQELACRHSVAHGSGTERNIQVGFKTTMKDHSVAGLNIEYLVIGHSFVANNNLGQSG
                     SGMPGSFDCEFEAIPASVQFRSRREVPRPFIPGTQSALVVADFSSDSDASTGGNMGRV
                     RVQFFWDRYGKQSCWARVSTPWAGKGYGFQNMPRVGEEVLVQFIEGDPDRPVVVGRVH
                     NAENMPPFKLPASAPFTGIKTLSIDSSGKSVAGKWNELRFDDSDGKEQIYFQAQKDFD
                     RRVLNDDKTWVGNESHLFIKADAFAKYGADHHVNTVGDHNEKIGGKQSTDVTSDIHVK
                     SGTMFVVDGGQEVHLKASSKIVLESPMMVSLVCGGNFVTLSPSGVDIKGIMVNVNGAG
                     SKGSGSAISPQPPKDAKEAMTSDGGTATAKPAAPPKPTTFSPKASSFKVAAATGAPFV
                     SSSCPG"
     gene            6701..7699
                     /locus_tag="VIN75_00795"
     CDS             6701..7699
                     /locus_tag="VIN75_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEY8972722.1"
                     /translation="MAHKRSPHPTGAHAAPAPAAATPAGSRARTVSEVPLAPGENTSG
                     QRKYKYAGGYDFDKIASGMAVKFKHNDHYDERSLPNMQVLLGMIEADALITDIRWTAY
                     MLATAMWETSHIESFQVPRTGKDGKVMTGKDGKVITRTRKVWINMKPVHETGEGAGRR
                     YHNPVKVAAQPTGELKITEWNGNVFTLKTNGEYTSAVHGRVPGTNPPARPVEAYTKAA
                     GDEHSYFGRGYVQLTWWYNYASAGVLLDKGLTLVTDPENAMIAQTAYDLMSLCMRTGK
                     GFANKHKFDDYFSGTQRDYEGARAMVNAADTESIAKVANIARLFEDVLWDARKSAS"
     gene            7725..>7992
                     /locus_tag="VIN75_00800"
     CDS             7725..>7992
                     /locus_tag="VIN75_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972723.1"
                     /translation="MLRGAAAIAGLLHAVAATAATGAPAAFAGDWIVTQVAVDQADPQ
                     NQVFKAQDDRLLNRVMTLDAGGRLSFNLTTEACDHVTWVRQPRAR"
BASE COUNT         1247 a         2675 c         2856 g         1214 t
ORIGIN      
        1 cagcaggtac tcgcgcaagc cggccgggtc gggatgctgc atcgtccggc ggatggtggg
       61 cacggtgttc agcagcaccg acgcgttggc caccagcggg ttcatgccca ccggcgcccc
      121 gccgtcctcg acgtcggaca gcacggccga ggtgtcgagc gtcatggcct ggcccgccga
      181 cggcgcgggc gtcgccgcgc ggcggccgcc cggcttgggg atcatcatcg tcgcgtcggg
      241 atcgtccgac ggcgggaaat tcggatcgct catgatggaa acgtgtcagt cagggcttga
      301 gcgcccagag ctcgagctgc aggcccggga aatcgccggc gatgtgcatc gcgatgacct
      361 tggtgacgtc gagctgcttc cacaggtcgc cgctgcgatc gagctggaaa taggtgtagc
      421 cggcgtagta cggcatgcgc ggcggggcca tcggcagcgg ttcgaccgcg atggcaggca
      481 ggtgcagtcg cacgaggtcg cggatcttgt cgggcgggcc gatcttgacg aacgacggga
      541 agcggctgcg cacgaactcg ccgggcttgt cggcgttgac ggccaggacg aactgcgcat
      601 tgcgcagcag gtcgccgtcc ggcaccatgc ccgtgtacag gcccgcggcg cgctcctgca
      661 gcgggatggg gaccgcgttc gggttgatca cctcggtgag cagttcgcgg atgtcgaaca
      721 tcagcggctc gaaggtgttc ttcaggtcct cgtggcgata gatgggctgc acgcgggcgc
      781 gacgcttgcg gcggtcgctg aaggtggcga actcgccggc cgcctgcacc agcgctgcgt
      841 acagcgcctc gggatgcagc gcctcgcgct cggacaggtg cgtgagccac gggtcgtagc
      901 ggttggcgac ctgcagcatc atgaagtcgc cgatctcgcc gacgcccttc tgctgcgact
      961 ggttcaggcg cgcggccagc gtgtcggcac gatggcgcac caggttgcgc gcctcgacca
     1021 ggtagtcctg aaggcgcgcg aagccgcggc aatcgagcgc cggcgggatg aagctctcgt
     1081 cgagcacgag ctggcccgtg ctgcggcgct cgatgatgcg tgccacggcg atgctgcaga
     1141 agcccgagcg cggctcgtcg tccagcatca ggcgcaggtt cagctggccc agcttcatgt
     1201 cggccggatc gccgacgccc tgcacgttgt cctcgacgcg caggtcctgg ccgacgaagc
     1261 gcgcgagcgc cgtggcgggc ccggcgtcga ccgcgaactc gggcatgccg gcacgcacca
     1321 gcggcaacgc gaggtggacg acggtgtcct tggtctcggt gccgatctcg agcgccagcg
     1381 gcgccgcgta ttcctcgggg atggcgaagg gcgtgccatc gggcatcacg ccgcgcgcgg
     1441 acttgatcga cagccgccct atcttcaggg cctcgtcatc gacgcgcagg ttggccagac
     1501 cccagccggg tgcgaacacg ccggtcgcgc gggactcgac gagcatctcg accgagcgtt
     1561 cgagttgctg gaaatgctgc ggccgcagga acaatccctc cgaccagatg acgcgctttc
     1621 ggctcatgct cttgttgtcg tgagaaccga cgccaacgac gtgcgcgggt tcaagttgac
     1681 gacccgggac ttggcggggc cgcagcaaat gtacacgcaa tcaccggggc cgccgagggt
     1741 atgaaacccc actttccccc ctcgtggaca ccgggctaag gattgacttc cggtgtcgat
     1801 ggcgccggct ggatcggcga tgacggaacg gacgtttcac aggaccgaac caagccgcgg
     1861 ccctcccgac gaccgcggtg cgacgccctc gccggcccag cggatcagcg ccagctgccg
     1921 gccagttcgc ctccggtggc cgcgatcgac cgccaggcgg tgacgcgcgc gttcggcacg
     1981 cgccggtcgc tttgcagcgt gatcaggccg gccgcctgca gcacgcccgc ctcgccctcg
     2041 ccgaggtagg cctcggccac ggcctgcagg cggggctcgt cgccgcttcg gtcgatgaag
     2101 gccggcaggt cctccatctg cacctcggtc tcgacaccgc cccgggtatg cccctgcgcc
     2161 agcagcaggg ctgcgtcgag ggcggcgctg cgccaggcca ggcggtcgtg cagcgaagca
     2221 ccggtcgagt cggcgagctc gtcgaacgcg aaggccgcca ccggatcgtg gcgcgggccg
     2281 tacggcagtc gcgcgagcag gcgcggaaac gtcaggccgg tgtgcggcgc gatcgcgctc
     2341 tcgcgcaacg cccgccacac gcgcgcttcg ttggacgcca gcacctgggc atcctggctg
     2401 gtggccaccg cggcgagcgc cggggcggcg ccggccagca gcacggcgcc ccaggcggcg
     2461 gcgacattgg ccaggccacc cagcagcgac agctcgggca cgctgggccc gaattcctcc
     2521 agcgagacga gcacgtcgca gccgcggcag tcgcccttga gcgccggggc gaggccgctg
     2581 cgggcggggt cgccggcgca ggccgacagg tcggccagca gttcgtcggc gcggcagtcg
     2641 aacagctcca ggcgcaccga gccgtcgtcc atgtccagcg tgcgcacgaa gcggtcgacc
     2701 gcgcgccacg cgcattcgag acggcgccat gccggatcgc gcagcacctg gcgcagcatc
     2761 ggcgcggcgg catcggtgac cgccgcggcg gccggcgccg gcgcgggcgc gctggcctgg
     2821 tcgccgatca gctggcggat gagccggtcg acgacggcat cgggccggtt cgatgtcgac
     2881 gcgggcgccg ccgccagcgc gggtgctccg gccggcaggt cgccgccgag cagacgcttc
     2941 atcaggtcgg ccgcgtcatc gccgccggtg gacgtcgcgg gcgccggcga tggcggcgtg
     3001 ccggcgcgcg cgagcgcgtc cgcgggcagg cgcgcgatga cgccgtcggc cgagaactcg
     3061 tccatcgtct cgacctcgat gcgcagcggc cggccctgct tgtcctgcag gcccgcgatc
     3121 tcggcgcgcg gcgcgacgcg ggccagctgg gcgtcgaagt tgtcgatgtc gatccgcagc
     3181 ggcaggcgct cgcccagcgg cgcgcgctcg tcgcgcgggc ggccggagaa gtcgccgagg
     3241 acgacgatgc ggaacggccc ggcggcgtcg tcgccggcgc ggtcacgacg gcccggggac
     3301 ggcacgcggc cgatgttcag ttcgatcgaa gacggcatgg cgggattcct cgcggggacg
     3361 gcgtcagccg atgctctggg cgacgagctt gccgtcggcc accgaccagg cgtgcgcgcg
     3421 cgtgggcacc tcgccctcgg cgatgtagaa gtcgaccggc ccgaagaact cgcccagctg
     3481 ggccaccggg cacgtgggca ggtagttgcg catcacccgc gggtcgtaga agcggaagaa
     3541 cagcgactcc atgtcggggc cgtagacgtg cacctgctgg cgcacgtgac gccacagcgc
     3601 cggcagcgcg agctggctcg tgaggaacac gccccagttc tggccccagc cctcggcgaa
     3661 cagccggcgc gtgaacgcgg cgctgtgctc gagctcgacc aggtagggcg cgacggtggc
     3721 catgtcgggc tcgagctggc cgccgaacag gcagcgccac ggcggcgcat ggtcggcata
     3781 gagcgcgtcc agcagcccgg ggttctgggc gccatcgagg atcacgtagg ccggcatgtc
     3841 gccggcttct tgccagaggc gggcctggac ggccgccggc atcggaaggg agcttgcgga
     3901 catgggttcc gatcagggaa tcgtgtgcgt catctgacct tccatctgga tggagatctg
     3961 tccggcccaa ttacacatca gcatcgagct cttgttcaat gcgggttggt tcgcgagcgt
     4021 gacggtgggc gagcccgcca cccacggcgc gggcgtcacg gggatgcagg gcatcggcgt
     4081 gagcacgccc atcgccgccg ccgtggccgc ggccaccatc ggattggagg gcgactggca
     4141 catcccgaac ggcatgatgt tgaccatcgg gatgtggtcc tggatgttgg ccgcgggcat
     4201 gttggacgtg ttctgcatgt tcagcggcaa cacgaccagg ctgctggggg ccatgcccat
     4261 ggtgcatttc agcagggcgc cgttacagac gtgattgggc atttccgatt ctcctcagga
     4321 acgggcgccg cgcggacgcg ccgcgcgggc cgcgggcgtc aacccgggca ggacgacgag
     4381 acgaacggcg cgccggtggc cgcggccacc ttgaagctgc tggccttggg cgagaacgtg
     4441 gtcggcttgg gcggcgccgc cggcttggcc gtggcggtgc cgccgtcgct ggtcatcgcc
     4501 tccttggcgt ccttgggcgg ctgcgggctg atcgcgctgc ccgagccctt gctgccggct
     4561 ccgttgacgt tgaccatgat gcccttgatg tcgacgcccg acgggctgag cgtgacgaag
     4621 ttgccgccgc acaccagcga caccatcatc ggcgactcca gcacgatctt ggagctggcc
     4681 ttcaggtgca cttcctggcc gccgtcgacg acgaacatcg tgccgctctt gacgtggatg
     4741 tccgaggtca cgtccgtgga ctgcttgccg ccgatcttct cgttgtggtc gccgacggtg
     4801 ttgacgtggt ggtcggcgcc gtacttggcg aacgcatcgg ccttgatgaa caggtgcgat
     4861 tcgttgccga cccaggtctt gtcgtcgttg agaacgcggc ggtcgaagtc cttctgcgcc
     4921 tggaagtaga tctgctcctt gccgtcggaa tcgtcgaagc gcagctcgtt ccacttgccg
     4981 gccacgctct tgccgctgga gtcgatgctc agcgtcttga tgccggtgaa cggcgccgag
     5041 gcgggcagct tgaacggggg catgttctcg gcgttgtgca cgcggccgac gaccaccggc
     5101 cgatccgggt cgccttcgat gaactgcacc agcacctctt cgccgacgcg gggcatgttc
     5161 tggaagccgt agcccttgcc ggcccacggc gtggagacgc gggcccagca ggactgcttg
     5221 ccgtagcggt cccagaagaa ctgcacgcgc acgcgtccca tgttgccgcc ggtgctggcg
     5281 tcgctgtcgc tgctgaaatc ggcgacgacg agcgccgatt gcgtgccggg gatgaacggg
     5341 cgcggcacct cgcgccggga gcggaactgc acgctggcgg ggatcgcctc gaactcgcag
     5401 tcgaagctgc cgggcatgcc gctgccggac tggcccaggt tgttgttggc gacgaacgag
     5461 tgcccgatca ccaggtattc gatgttgagc ccggcgaccg agtggtcctt catcgtcgtc
     5521 ttgaagccga cctggatgtt gcgctcggtg cccgagccgt gggcgacgct gtggcggcac
     5581 gccagctcct gggcccggat cgacgcatag ctggtggcat cggccgcggt gccggtgtcg
     5641 atcggatagt cgaacatctc gaagccggcg ttgtcgtgcg acttcgggtg cgagtcgact
     5701 gccgcgatct gggtgttggg cgtgacgtag ttgtagtcgc cggtggcaaa ggcgccggac
     5761 tgcacctggg agatcagcgt gaagcccgtc accgtgccgt ccgtgtcggc cgggccgtgg
     5821 aagctgacgg tggacagtcc cggcgtggtc ttgtggctgc cggcgtcgtt gagcagcacc
     5881 atcttgtggc tgccgttgtc gtgcgtgaag tagtagtaga tgcctgcgtg ctcggccagc
     5941 cggctgacga aggcgaaatc ggtctcgcgg tactgcacga cgaaatcgcg ctgctcggtc
     6001 gagccgacct ggttgtcgat cgtcatcagc gtgtcgacgt tgagctgctg cgcaatcagg
     6061 tcgccgatgg tgatcgtcga gaagatctgg ctgttggacg agcgcgtgcg gaaccacacg
     6121 cccggatgca gcgtcacctt atacaggaac ccggtattgc tcttgaacgc cggcgtgctg
     6181 acggagccga gcaccgagaa gctcgtgacg tagccgttga agtagcgcga tgcctggccg
     6241 tccatcaact cgagcgccac cgtggcattc gagcccagca tctggtccga ggtgatgtcg
     6301 cctcgctcgg agacgagatc gagctggtac tcgtagaggc gccccagttc ctcgcgtccg
     6361 ctgaagcgct ggacgacgaa aacgtccgcg ccaaccttgg tcgagatttc gagcatgcgt
     6421 ttcatggaaa gggctcctgc gagaattcgt cgcgacggcc gggtcatcgg gcacgcgcgg
     6481 ccgacgaatc catttcaaca cggccaacgc ctgcacggca gccgccaaag tcgcgatttc
     6541 cacctagatg gcaccggcaa tgcgccgcgt gccggcggga cgcggggatg gtacgccaac
     6601 gcgagccgcg cctcgaaccc cagagagggg gattgaccct gcgagagggc gcgccgctat
     6661 agttgacgcg atcgaaggga ccgcggggtc gcccgtcacc atggctcaca aacgctcgcc
     6721 tcatcccacc ggcgcccacg ccgctcctgc ccccgccgcc gccacgcccg ccggttcgcg
     6781 cgcgcgcacc gtctccgaag ttccgctggc acccggcgag aacaccagcg gccagcgcaa
     6841 gtacaagtac gcgggcggct acgacttcga caagatcgcg agcggcatgg cggtcaagtt
     6901 caagcacaac gaccactacg acgagcgctc gttgccgaac atgcaggtgc tgctcgggat
     6961 gatcgaggcc gatgcgctca tcaccgacat ccgctggacg gcctacatgc tggccacggc
     7021 catgtgggag accagtcaca tcgagtcgtt ccaggtcccg cgcaccggca aggacggcaa
     7081 ggtgatgacc ggcaaggacg gcaaggtgat cacgcggacc cgcaaggtct ggatcaacat
     7141 gaagccggtt cacgagaccg gcgagggcgc gggccggcga tatcacaacc cggtcaaggt
     7201 ggcggcgcag cccaccggcg agctgaagat caccgaatgg aacggcaacg tcttcacgct
     7261 caagaccaat ggcgagtaca cgtcggccgt gcacggccgg gtgcccggca ccaatccgcc
     7321 ggcccggccg gtcgaggcct acaccaaggc ggccggcgac gaacacagct acttcgggcg
     7381 cggctacgtc cagctgacct ggtggtacaa ctacgcgagc gccggcgtgc tcctcgacaa
     7441 gggcctgacc ctggtgaccg atcccgagaa cgcgatgatc gcccagaccg cctacgacct
     7501 gatgtcgctg tgcatgcgca ccggcaaggg attcgccaac aagcacaagt tcgatgacta
     7561 cttctcgggc acacaacgcg actacgaagg cgctcgcgcc atggtcaacg cggccgacac
     7621 cgagagcatc gccaaggtcg ccaacatcgc gcggctgttc gaggacgtgc tctgggatgc
     7681 gcgcaagtcc gcgagctgag ccgccgaagc gctgcctgcg gacgatgctc cgaggcgccg
     7741 ccgccatcgc cggcctgctg cacgcggtgg ccgccaccgc ggcgaccggc gcgcccgccg
     7801 cgtttgcggg cgactggatc gtcacgcagg tggccgtcga ccaggccgac ccgcagaacc
     7861 aggtcttcaa ggcgcaggac gaccggctgc tcaaccgcgt gatgacgctg gacgccggcg
     7921 gccgcctgtc gttcaacctg accaccgaag cctgcgacca cgtgacctgg gtccgccagc
     7981 cgcgcgcgcg gc
//
LOCUS       DATCRO010000030         5929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_754097, whole genome shotgun sequence.
ACCESSION   DATCRO010000030 DATCRO010000000
VERSION     DATCRO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754097"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VIN75_00805"
     CDS             <1..379
                     /locus_tag="VIN75_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972724.1"
                     /translation="ADSVARSSAGAPAANAAAEPGAAREAAEADVARASALPESRMVA
                     PQPTERLGTGHGERESSVARMTTFERATPQPEQRVEIRYDSYENLAAAGIVPPPRAAW
                     TPPPHAFPADPPRGYVPDPPMVR"
     gene            445..1137
                     /locus_tag="VIN75_00810"
     CDS             445..1137
                     /locus_tag="VIN75_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8972725.1"
                     /translation="MQAHPDSVPPLAFAREADERPWEQAPVATAAPAVDSDEFLMLAF
                     AGGDTAAFTRLYDRHERATWRFIRHRLGPQHEGAADDVLQETWISVARAASRYVATAR
                     FTTWLFTVARNRVIDHLRAQSNAMQSIDAPLAGAGSQDDGERWADRVAADASEGPQAR
                     IESREQAQAFLDALAQLPEAQREAFVLQAEGGLSVEEVASATGVGTETAKTRLRYAKA
                     RLRTLLAGWRTP"
     gene            1134..2546
                     /locus_tag="VIN75_00815"
     CDS             1134..2546
                     /locus_tag="VIN75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8972726.1"
                     /translation="MSEPTPGRPMDDDLDQAYARSHALAGDDRGPSASVRANVLAAAA
                     RIAAGEAAEAAPRAPLAPVAPPVEAVDGKRGPAVNLSSWRVRSGAALCALLLVCAGAW
                     RFDQNGRLGGGVQVALAELRLAEPRTAPAPAATQELPVPAAAAASYPYAAPPAVVVDP
                     FDGGAGTHGPTARHAERERDVVVAQLDEQRAAAERQPAPVDKASLSARAVQPGLARSP
                     AAADAAAPPRPVALASNGPRGEGSPTEQESATVTITAAAPAAPPTIMTSPARPPSVLP
                     RRVMLVPRPASAPSPRSAATAGDTVVASADTGASARDGRFAEISPAPPPMAKAAVAPP
                     AAPVLAAAAPPSPMVLDKPEGRVSSDADAIRRLKTAAPGIPRASAAPDSLQAAADRGD
                     VDALKALLAGPAARVDATDADGRTALLHAVLAQRPAAVRLLLAAGADPGRADHAGLTP
                     RQAAQVGGNAEIAALLGATR"
     gene            2691..3611
                     /locus_tag="VIN75_00820"
     CDS             2691..3611
                     /locus_tag="VIN75_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8972727.1"
                     /translation="MLSIASLTHVYPNGTRALDSVSLDIPPGLYGLLGPNGAGKSTLM
                     RCLATLQVPTSGQIRFGDIDVLAEPQKLRARLGYLPQDFGVYPRVSAWEMLDHLAVLK
                     GLTDAGARRETIEALLQQVNLWDVRKKAIAGFSGGMRQRFGIAQAMIGNPALMIVDEP
                     TAGLDPEERNRFNNLLAEIGTTKVVILSTHIVEDVADLCSRMAILVDGRIVSAGTPGA
                     LIAGLEGKVWRRAVDAEELPGLRKQFALISTRLRGGRTLIHVLSDTAPDPRFEKAEVD
                     LEEVYFSTLYAHRRSNGQSGNEASQGAAAR"
     gene            3627..>5929
                     /locus_tag="VIN75_00825"
     CDS             3627..>5929
                     /locus_tag="VIN75_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972728.1"
                     /translation="MFGHIVKFEWRYQLRSPVFWVGCLIFFLLAFGATASDNVQIGSL
                     GNVHKNAPYAIVQALALLGVFSVFITVAMVANVVVRDDDTGFAPIIRTTSIDKASYLG
                     GRFVGASAAAFVVLAMVPLGIWVGTLMPWQDVEKIGPFVAGHYLVGLFAFELPTLLIT
                     AALFFAVAIATRSLVWSNVCAVAFLILFFVARGATRNDPAWERVAALLDPFGPSPLNY
                     VTKYWTTSERNTQLPPFTGVLLANRLLWTAVAGAVFAIAYRLFRFETRQDAPAADTAE
                     DAPAKPRKLTRAQKAALRHQAELAVLGEANPAGVREMLAAEARRNVPALPDIPPATRG
                     TARLQLWELAKVDMAFVFRSPVYYVLVAIGLLLAGSNLVFGGEILGSPSWPVTRLMVE
                     ALGSGYSLLPIILAIFYAGELVWRDRDRRMHEIIDATGAPDWTHLVPKVIAIAAVLVS
                     SALLAVLAAIAFQALNGYFRFEIGAYLAWFVWPMAVGAILIAVLTVFVQVLVSNKYIG
                     WAVMLVYFVASVMLGNVGWEHNLYNYGGSPQIPLSDMNGLGRFWIGQAWFQLYWAAFA
                     TMLLVLSHALWRRGTTVALRPRLAQIGRHLRGTPAAVLGGAALVWIASGAWIFYNTNV
                     LNLYTTQPERDRRAADFEKTLLPFEKVAQPRVADVTLRVEIWPRDARAVTHGSFALVN
                     RSSEAIPVLHLQWAENLRLDSITFPGATVQTDYPRFHYRIYRLATPMQPGERRVLDFT
                     TTLEQRGFRNDRPMTAIVPNGSFFDNYA"
BASE COUNT          762 a         2244 c         2114 g          809 t
ORIGIN      
        1 ggcggacagc gtcgccaggt cctccgccgg cgcgcctgcc gccaatgcgg ccgccgagcc
       61 gggcgcggcg cgcgaagccg ccgaggccga cgtcgcccgc gcgtccgcac tgcccgagtc
      121 ccgcatggtc gcgccgcagc cgaccgagcg cctgggcacc ggccacggcg agcgcgaatc
      181 ctcggtcgcg cgcatgacca cgttcgagcg ggcgacgccg cagcccgagc agcgcgtgga
      241 gatccgctac gacagctatg agaacctggc cgccgccggc atcgtcccac cgccccgcgc
      301 ggcctggacg ccaccgccgc acgccttccc cgccgatccg ccgcgcggct acgtccccga
      361 cccgccgatg gtgcgctgac tcgcttggac ccgtcgccga tccggtggcc tgaaccacaa
      421 ccgcacgggt tagcatcgtg gcccgtgcag gcccaccccg actccgttcc gccgctcgcg
      481 ttcgcgcgcg aggcggacga acgcccgtgg gaacaggcgc cggtggccac cgccgccccc
      541 gccgtcgaca gcgacgaatt cctgatgctt gcctttgccg gcggcgacac cgccgccttc
      601 acgcgcctgt acgaccgcca cgagcgggcg acctggcgct tcatccgcca ccggctcggg
      661 ccgcagcacg agggcgcggc cgacgacgtc ctgcaggaga cgtggatcag cgtcgcccgt
      721 gccgcgtcgc gctacgtggc caccgcgcgc ttcaccacct ggctgttcac cgtggcccgc
      781 aaccgcgtca tcgaccacct gcgcgcgcag tccaacgcga tgcagtccat cgacgccccg
      841 ctggccggcg ccgggtcgca ggacgacggc gagcgctggg ccgaccgcgt ggcggccgac
      901 gcgtccgagg ggccgcaggc gcgcatcgag tcgcgcgagc aggcgcaggc cttcctcgac
      961 gcgctggcgc agctgcccga ggcgcagcgc gaggccttcg tgctgcaggc cgagggcggc
     1021 ttgagcgtcg aggaggtggc cagcgccacg ggcgtcggca cggagacggc caagacgcgg
     1081 ctgcgctacg ccaaggccag gctgcgcacg ctgctggccg gatggaggac gccatgagcg
     1141 agccgacgcc gggacgcccg atggacgacg acctcgacca ggcctacgcg cgttcgcacg
     1201 cgctcgccgg cgacgatcgt ggcccgtccg cgtcggtacg cgccaacgtg ctggccgccg
     1261 ccgcgcgcat cgccgcgggc gaggcggccg aggccgcgcc gcgggcgccg ctggcgccgg
     1321 tcgccccgcc cgtcgaggcg gtcgacggca agcgcgggcc ggcggtcaac ctgtcgtcgt
     1381 ggcgcgtgcg ttcgggcgcg gccctgtgcg cgttgctgct ggtgtgcgcc ggcgcctggc
     1441 gcttcgacca gaacggccgc ctgggcggcg gcgtgcaggt ggcgctggcc gagctgcggc
     1501 tcgccgagcc gcgcacggcc ccggcgccgg cggccacgca ggagctgccg gtgccggctg
     1561 ccgcggctgc gtcctatccg tatgcagcgc cgccagcggt ggtcgtcgac ccgttcgacg
     1621 gcggcgccgg cacgcatggt ccgaccgcca ggcacgcgga gcgcgagcgc gacgtggtcg
     1681 tcgcgcagct ggacgagcag cgcgccgccg cggagcgcca gccggcgccg gtcgacaagg
     1741 cttcgctgtc ggcccgcgcc gtgcagccgg gcctggcgcg ctcgcccgcc gcggcggacg
     1801 cggccgcgcc gccgcggccg gtggcgctcg cctcgaacgg gccgcgcggc gagggcagtc
     1861 cgaccgagca ggagtcggcc accgtgacga tcaccgccgc ggcgccggcc gcgccgccga
     1921 ccatcatgac ctcgccggcg cggccgccgt cggtgctgcc gcggcgcgtg atgctcgtcc
     1981 cgcgcccggc gtcggcgccg tccccgcgtt ccgccgcgac tgcgggcgac acggtggtcg
     2041 cctcggcgga cacgggcgca tccgcacgcg acggccggtt cgccgagatc tcgccggccc
     2101 cgccgcccat ggccaaggcc gccgtcgcgc cgcccgccgc gcccgtcctg gccgccgcgg
     2161 cgccgccgtc gcccatggtg ctggacaagc cggagggccg cgtctcgtcc gatgccgacg
     2221 cgatcaggcg cctgaagacc gccgcgccgg gcatcccgcg ggcgtccgct gcgcccgact
     2281 ccctgcaggc ggccgccgat cgcggcgacg tcgacgcgtt gaaggcgctg ctggccggcc
     2341 cggccgcccg cgtcgacgca accgacgccg acggccgtac cgcgctgctg cacgcggtcc
     2401 tggcgcagcg cccggcggcc gtgcggctgc tgctggccgc gggcgccgac ccgggccgcg
     2461 ccgaccacgc cggcctgacg ccgcgccagg cggcgcaggt cgggggcaac gccgagatcg
     2521 cggcgctgct gggcgcgacg cgctgatcca ccttgccgcg gcagggcacg gtcgccggac
     2581 ggcctgtccc agggaagcgc cggttcgtcg cacggaggtg gggccggcgg cgggctgccg
     2641 tcagaatgcc aacctggaaa gtccaagaat ccgtccccgg agccgcccgc atgctgtcca
     2701 tcgcctcgct gacccacgtc tacccgaacg gaacgcgggc gctcgacagc gtgtcgctgg
     2761 acatcccgcc cgggctgtac ggcctgctgg ggcccaatgg cgcaggcaag tcgacgctga
     2821 tgcgctgcct ggccacgctg caggtgccca cctccggcca gatccgcttc ggcgacatcg
     2881 acgtgctggc cgagccgcag aagctgcgcg ccaggctggg ctacctgccg caggacttcg
     2941 gcgtctatcc gcgcgtgtcg gcctgggaga tgctggacca cctggccgtg ctcaagggcc
     3001 tgaccgacgc cggtgcgcgc cgcgagacga tcgaggcgct gctgcagcag gtcaacctct
     3061 gggacgtgcg caagaaggcc atcgccggct tctcgggcgg catgcgccag cgcttcggca
     3121 tcgcgcaggc catgatcggc aacccggcgt tgatgatcgt cgacgagccg accgccggcc
     3181 tcgaccccga ggaacgcaac cgcttcaaca acctgctggc cgagatcggc acgaccaagg
     3241 tggtgatcct gtcgacacac atcgtcgagg acgtcgccga cctgtgctcg cgcatggcca
     3301 tcctcgtcga cggccgcatc gtcagcgccg gcacgccggg ggcgctgatc gccgggctcg
     3361 agggcaaggt ctggcgccgc gccgtcgatg ccgaggagct gcccgggctg cgcaagcagt
     3421 tcgcgctgat ctccacgcgc ctgcgcggcg gccgcacgct catccacgtg ctgtccgaca
     3481 ccgcgcccga cccgcggttc gagaaggccg aggtggatct cgaggaggtc tacttctcga
     3541 cgctgtacgc gcaccgccgc agcaacgggc aatcgggcaa cgaggcgtcg caaggcgccg
     3601 cggcgcgctg acggacgggt gcccccatgt tcggtcacat cgtcaagttc gaatggcgct
     3661 accagctccg cagcccggtg ttctgggtcg ggtgcctgat cttcttcctg ctcgcgttcg
     3721 gcgccacggc cagcgacaac gtgcagatcg gcagcctggg caacgtgcac aagaacgcgc
     3781 cgtacgcgat cgtgcaggcg ctcgcgctgc tgggcgtgtt ctcggtcttc atcaccgtgg
     3841 ccatggtggc caacgtcgtc gtgcgcgacg acgacaccgg ctttgccccg atcatccgca
     3901 ccacgtcgat cgacaaggcg agctacctgg gcggccgctt cgtcggcgcg agcgcggcgg
     3961 ccttcgtcgt gctggcgatg gtcccgctgg gcatctgggt gggcaccctg atgccctggc
     4021 aggacgtcga gaagatcggg ccgttcgtgg ccggccacta cctcgtgggc ctgttcgcgt
     4081 tcgagctgcc gacgctgctg atcaccgccg cgctgttctt cgcggtggcc atcgccacgc
     4141 gctcgctggt gtggtccaac gtctgcgcgg tcgccttcct gatcctgttc ttcgtggcgc
     4201 gcggcgcgac gcgcaacgac ccggcgtggg aacgcgtcgc ggcgctgctg gacccgttcg
     4261 gcccgtcgcc gctgaactac gtcaccaagt actggacgac ctccgagcgc aacacgcagc
     4321 tgccgccgtt caccggcgtg ctgctggcca accggctgtt gtggacggcg gtcgcgggcg
     4381 cggtgttcgc gatcgcctac cggctgttcc gcttcgagac gcggcaggac gctcccgcgg
     4441 ccgacaccgc cgaggacgcg cctgccaagc cgcgcaagct gacacgcgcc cagaaggccg
     4501 cgctgcgcca ccaggccgaa ctggccgtgc tgggcgaggc caaccccgcc ggcgtgcgcg
     4561 agatgctggc cgccgaggcg cgccgcaacg tgcccgcgct gccggacatc ccgcccgcca
     4621 cgcgcggcac cgcccgcctg cagttgtggg agctggccaa ggtcgacatg gccttcgtgt
     4681 tccgcagccc tgtctactac gtgctggtcg ccatcggcct gctgctggcc ggctccaacc
     4741 tggtgttcgg cggcgagatc ctcggcagcc cgtcctggcc ggtcacgcgc ctgatggtgg
     4801 aggcactggg cagcgggtat tcgctgctgc cgatcatcct ggcgatcttc tacgcggggg
     4861 aactggtgtg gcgcgaccgc gatcgccgca tgcacgagat catcgatgcc acgggcgcgc
     4921 ccgactggac gcacctcgtg cccaaggtca tcgcgatcgc ggcggtgctg gtctcgagcg
     4981 cactgctggc ggtgctcgcg gccatcgcgt tccaggcgct gaacggctac ttccgcttcg
     5041 agatcggcgc ctacctggca tggttcgtgt ggccgatggc ggtcggcgcg atcctcatcg
     5101 ccgtcctgac ggtgttcgtg caggtgctcg tgtccaacaa gtacatcggt tgggcggtga
     5161 tgctcgtgta cttcgtggcc tcggtgatgc tcggcaacgt cggctgggag cacaacctct
     5221 acaactacgg cggcagcccg cagatccccc tgtcggacat gaacggcctg ggccgcttct
     5281 ggatcggcca ggcctggttc cagctgtact gggcggcgtt cgcgacgatg ctgctggtgc
     5341 tgtcgcatgc gctgtggcgc cgcggcacga ccgtcgcgct gcgcccgcgc ctcgcgcaga
     5401 tcggccggca cctgcgcggc acgccggcgg ccgtgctggg cggcgcggcg ctcgtctgga
     5461 tcgcctcggg cgcctggatc ttctacaaca ccaacgtgct gaacctctac acgacgcagc
     5521 ccgagcgcga ccgccgcgcc gccgacttcg agaagacgct gctgccgttc gagaaggtgg
     5581 cgcagccgcg cgtggccgac gtcacgctgc gcgtggagat ctggccgcga gacgcccgcg
     5641 ccgtcacgca tggcagcttc gcgctggtca accgcagcag cgaggccatc cccgtgctgc
     5701 acctgcaatg ggccgagaac ctgcggctgg actcgatcac gttccccggc gccacggtgc
     5761 agaccgacta cccgcgcttc cactaccgca tctacaggct ggccacgccg atgcagccgg
     5821 gcgagcgccg agtgctggac ttcaccacca cgctggagca gcgtggcttt cgcaacgaca
     5881 ggccgatgac ggcgatcgtg cccaacggca gcttcttcga caactacgc
//
LOCUS       DATCRO010000031         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_754098, whole genome shotgun sequence.
ACCESSION   DATCRO010000031 DATCRO010000000
VERSION     DATCRO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754098"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..635
                     /locus_tag="VIN75_00830"
     CDS             138..635
                     /locus_tag="VIN75_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8972729.1"
                     /translation="MSTTASRAPTAQDALELLSDEQDQILELFDRYDALVAEAAEADG
                     RRELAQQICSLLLVHVQAKREILYPAARAALDDETLVDETIESHAALEESIADVQAGD
                     ATEPRYDAGVRVLQEQFVDAMEQERVSLFPALRASSIDLEELGGELGALEERLLDADD
                     ELGSM"
     gene            complement(818..3058)
                     /locus_tag="VIN75_00835"
     CDS             complement(818..3058)
                     /locus_tag="VIN75_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8972730.1"
                     /translation="MHPLMHAMREKMLEELQARVPTAPAGVTRRSVLASGAAGGLVVA
                     VGWLPGTAQAVAGPGAEGSAAKAALVPNQFVSVGTDNLVTIVCKHHEMGEGNTTGLAT
                     LVADELDADWSLVRTEYAPADAKRYANLAFGTMQGTGGSTAIANSYMQYREAGATARA
                     MLVAAAADAWKVPAGEIQARASVLTHPSGKRATYGEMAAAAARQAPPPKPALKSPAQF
                     TLMGHDRATPRVDSPSKCTGTAIYTGDVRLPGLLTAVIAFPPSFGARMVSFDATAARK
                     VKGVTDVVAVPEGVAVVAKNTWAAMQGRRALKVTWDESAGASLDSEALLAHYRELASQ
                     PGTPFAKPATPPAAAARTVEAIYEFPFLAHAPMEPQGCVAWLHDGQLETWAGHQFPTF
                     DHMLAAKAAGLPMDKVKLHSLVSGGSFGRRANVWSDFTVAAVNVAVAIKGRAPVRLQY
                     SREDDMSAGLYRPMMLHSLKVGLDGAGKVASWQHVVVGQSIVAGTPMAAMMVKDGVDP
                     TSIEGVHPTSYDLPMMDGQVHDVALPVRPLWWRSVGNTHTAYVMETMMDRLARAANQD
                     PLAFRLALLQKNERATRTLRLVADKAGWGKAMPPGSAQGIALHESFGTAVAQVAEVTL
                     KDGKVRVDKVWCAVDCGVAVNPDVIRAQMEGGIGFALGALYYSEIELKAGRPQQRNFD
                     QYRSLRIYEMPAIEVVIADSSAPPTGVGEPGVPPLAPAVANAVVRAGGPDVLRLPFTR
                     AGLVHA"
     gene            complement(3070..3534)
                     /locus_tag="VIN75_00840"
     CDS             complement(3070..3534)
                     /locus_tag="VIN75_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8972731.1"
                     /translation="MTTVSLNVNGHAHKVQADADTPLLWVLRDDIGLTGTKFGCGVAQ
                     CGACTVHLDGQPARSCVTPATAVGARKVTTIEGLQSRAAKAVQKAWIEHDVVQCGYCQ
                     SGQIMSATALLEQNPRPSDEDIDHAMAGNICRCATYVRIRAAIHAAAGQLSA"
     gene            3606..>4016
                     /locus_tag="VIN75_00845"
     CDS             3606..>4016
                     /locus_tag="VIN75_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HEY8972732.1"
                     /translation="MRPTTLVGFLLAAGLAAPAVHAQDLKSPADFAGIADPAERSRAL
                     FAEAGKVIQSPRCQNCHPVGDRPTQGDDMHPHLPLVVRGKDDQGAIAMRCATCHQVAN
                     FQPAGVPGAPKWHIAPVEMAWQGKSLGAICEQIKD"
BASE COUNT          521 a         1446 c         1460 g          589 t
ORIGIN      
        1 ccgggggttg ccgcgcttct ttgccccgag gccggccgcg gtgcgggttg caggggcatg
       61 cgcgttgccg tacaacacgt tgcggcgtgc gcggccagtc cgtgcggcgc cggccccgtg
      121 gccatcaagg agtccgcatg agcaccaccg catcgcgcgc gcccaccgcc caggacgccc
      181 tggagctgct gtccgacgag caggaccaga tcctcgaact gttcgatcgc tacgacgcgc
      241 tcgtggccga ggccgccgag gccgacggcc ggcgcgaact ggcccagcag atctgctcgc
      301 tgctgctcgt gcacgtgcag gccaagcgcg agatcctcta cccggccgcg cgcgccgcgc
      361 tggacgacga gacgctggtc gacgagacga tcgaatcgca tgccgcgctg gaggaatcga
      421 tcgccgacgt gcaggcgggc gacgccaccg agccgcgcta cgacgccggc gtgcgcgtgc
      481 tgcaggagca gtttgtcgac gcgatggagc aggagcgcgt cagcctgttc ccggcgctgc
      541 gcgcgagctc gatcgacctc gaggagctcg gcggcgagct gggcgcgctc gaggagcggc
      601 tgctggacgc ggacgacgag ctcggctcga tgtgatggcg ccgcgcgccg gtcgcgcgct
      661 cgcgcgacgc ggtgcgcggg ccggccttgc cgggtcgccg gcgatgcttc tgcgaacgac
      721 gaggccggcg cttcgcgcga cgccccgttg acgcatcgcg cccacgtggt gtcatcctgc
      781 gcgcagccgc aggatgcgcg cgtgcccgat gcgcccctca ggcgtgcacc agccccgccc
      841 gcgtgaacgg cagccgcagc acgtccggcc cgccggcgcg cacgactgcg ttggccacgg
      901 cgggagccag cggcgggacg cccggctcgc cgacgcccgt gggcggcgcg ctgctgtcgg
      961 cgatgaccac ctcgatggca ggcatctcgt agatgcgcag cgagcggtac tggtcgaagt
     1021 tgcgctgctg cggccggccg gccttcagct cgatctcgct gtagtagagc gcgccgagcg
     1081 cgaagccgat gccgccttcc atctgggcgc ggatcacgtc ggggttgacg gccacgccgc
     1141 agtcgaccgc gcaccacacc ttgtcgacgc gcaccttgcc gtccttgagc gtgacctcgg
     1201 ccacctgcgc gacggcggtg ccgaagctct cgtgcagcgc gatgccctgt gccgatcccg
     1261 gcggcatcgc cttgccccag ccggccttgt cggccaccag cctgagcgtg cgcgtggcgc
     1321 gctcgttctt ctgcagcagc gccagccgga acgcgagcgg atcctggttc gcggcgcgcg
     1381 cgagccggtc catcatcgtc tccatcacgt aggccgtgtg ggtgttgccg accgagcgcc
     1441 accacagcgg gcgcaccggc agcgcgacgt cgtgcacctg gccgtccatc atcggcaggt
     1501 cgtaggacgt ggggtgcacg ccctcgatcg aggtcgggtc gacgccgtcc ttgaccatca
     1561 tcgccgccat cggcgtgccc gcgacgatgg actggccgac caccacgtgc tgccacgacg
     1621 ccaccttgcc ggcgccgtcc aggcccacct tcagcgagtg cagcatcatc ggccggtaca
     1681 ggccggcgct catgtcgtcc tcgcggctgt actgcaggcg caccggcgcg cggcccttga
     1741 tcgccaccgc cacgttgacg gcggccacgg tgaagtcgct ccacacgttg gcgcggcggc
     1801 cgaagctgcc gcccgagacc agcgagtgca gcttgacctt gtccatcggc aggcctgccg
     1861 ccttggccgc gagcatgtgg tcgaaggtgg ggaactggtg gccggcccac gtctccagct
     1921 ggccgtcgtg cagccacgcg acgcagccct gcggctccat cggcgcgtgc gcgaggaacg
     1981 ggaattcgta gatcgcctcc accgtgcgcg cggcggccgc cggcggtgtc gcgggcttcg
     2041 cgaacggcgt gccgggctgg ctcgcgagct cgcggtagtg cgccagcagc gcctcggagt
     2101 cgagcgaggc gccggccgat tcgtcccagg tcaccttcag cgcgcgccgg ccctgcatcg
     2161 cggcccacgt gttcttcgcg acgaccgcca cgccttcggg cacggcgacg acgtcggtga
     2221 cgcccttgac cttcctggca gcggtggcat cgaacgacac catcctggcg ccgaaggacg
     2281 gcgggaacgc gatcaccgcg gtgagcaggc ccggcagcct gacgtcgccg gtgtagatgg
     2341 cggtgcccgt gcacttggac ggcgagtcca cgcgcggcgt ggcgcggtcg tggcccatca
     2401 gcgtgaactg cgccgggctc ttgagcgccg gcttgggcgg cggcgcctgc ctggcggcgg
     2461 ccgccgccat ctcgccatag gtggcgcgct tgcccgacgg gtgcgtcagc acgctggcgc
     2521 gcgcctggat ctcgccggcc ggcaccttcc aggcgtcggc ggcggccgcg accagcatcg
     2581 cgcgcgccgt ggcgccggcc tcgcggtact gcatgtagga attggcgatg gccgtcgatc
     2641 cgcccgtgcc ctgcatggtg ccgaacgcga ggttggcgta gcgcttcgcg tcggccgggg
     2701 cgtattcggt gcgcaccagc gaccagtcgg cgtcgagctc gtcggccacc agcgtggcca
     2761 ggcccgtggt gttgccttcg cccatctcgt ggtgcttgca gacgatggtg acgaggttgt
     2821 cggtgccgac gctgacgaac tggttgggca ccagggcggc cttggccgcg ctgccctcgg
     2881 cgcccgggcc cgcgacggcc tgcgcggttc cgggcagcca gccgacggcc acgacgaggc
     2941 cgccggccgc gccggacgcg agcacgctgc gtcgggtgac gcccgcgggc gcagtgggca
     3001 cccgggcctg gagttcctcg agcatcttct cgcgcatggc gtgcatcaac gggtgcatgg
     3061 cggactcctt caggccgaga gctggccggc cgcggcgtgg atcgcggcgc ggatgcggac
     3121 gtaggtggcg cagcggcaga tgttgccggc catcgcgtgg tcgatgtcct cgtcgctggg
     3181 ccgggggttc tgttccagca gcgccgtggc ggacatgatc tggccggact ggcagtagcc
     3241 gcactgcacg acgtcgtgct cgatccaggc cttctgcacc gccttggcgg ccctggactg
     3301 cagcccctcg atggtggtga ccttgcgggc gccgaccgcc gtggccggcg tgacgcagga
     3361 gcgcgcgggc tggccgtcca ggtgcaccgt gcaggcgccg cattgcgcga cgccgcagcc
     3421 gaacttggtg ccggtcaggc cgatgtcgtc gcggagcacc cacagcagcg gggtgtccgc
     3481 atcggcctgc accttgtgcg catggccatt gacgttgagc gaaacggtcg tcatcacgcg
     3541 gctcccgtga aggaattcgg aggccgcagt atgctcgccg cttggaattt ttccaggggg
     3601 ttcgaatgcg accgacaacg ctggttggat ttctcctcgc ggccggcctg gctgcgcccg
     3661 ccgtccatgc gcaggacctg aagtcacccg ccgacttcgc cggcatcgcc gaccccgccg
     3721 aacgctcgcg cgcgctgttc gccgaggccg gcaaggtgat ccagagcccg cgctgccaga
     3781 attgccatcc ggtgggcgac cgcccgaccc agggcgacga catgcacccg cacctgccgc
     3841 tggtggtgcg cggcaaggac gaccagggcg ccatcgcgat gcgctgcgcc acctgccacc
     3901 aggtcgccaa cttccagccc gccggcgtgc ccggcgcgcc caagtggcac atcgcgccgg
     3961 tggagatggc ctggcagggc aagtccctcg gcgcgatctg cgagcagatc aaggac
//
LOCUS       DATCRO010000032        15368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_416554, whole genome shotgun sequence.
ACCESSION   DATCRO010000032 DATCRO010000000
VERSION     DATCRO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416554"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2611
                     /gene="ppsA"
                     /locus_tag="VIN75_00850"
     CDS             212..2611
                     /gene="ppsA"
                     /locus_tag="VIN75_00850"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY8972733.1"
                     /translation="MSNPALATRLVIPFEELRNTDVDIVGGKNASLGEMISQLATTGV
                     RVPGGFATTAHAFRQFLEFGGLTQRIAARLKSLDTDDVRALAEAGSQIRQWIEDAPFP
                     PELEKGVREEFARLSASDPNASFAVRSSATAEDMPDASFAGQQETFLNVVGIDAVLQK
                     MREVFASLYNDRAISYRVHKGYADAEVALSAGVQRMVRSDLGAAGVMFTLDTESGFTD
                     VVFITASYGLGETVVQGAVNPDEFYVHKPALTAGKFPIIRRTLGSKLIRMEFASAEEK
                     ARDGKLVRTVDTPPEQRNRYSLSDADVVELAKYAMIIEKHYGRPMDIEWGKDGGDGLI
                     YILQARPETVKSNAASQAEHRYKLKGSGTVLVEGRAIGQKIGTGPVRVCRNVSEMDQV
                     QPGDVLVADMTDPNWEPVMKRAGAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDVL
                     HDGALVTVVCSEGDTGYVYDGLLETEVSEVVRGEMPYSAIKIMMNVGNPQLAFNFSQL
                     PNSGVGLARLEFIINNNIGVHPKAILDYPNIDVDLKKAVESVARGHASPRAFYVDKLA
                     EGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYISGEF
                     SDAFAMECEALRRVRHDMGLTNVEIMVPFVRTVRQAERVVTMLAERGLKRGVDGLRVI
                     MMCEVPSNAILADQFLEHFDGMSIGSNDLTQLTLGLDRDSGLEQLAEDFDERDPAVKA
                     MISRAINACRATGKYVGICGQGPSDHPDFAAWLADEGIVSISLNPDTVVDTWTALAAR
                     K"
     gene            2997..3365
                     /gene="rpsF"
                     /locus_tag="VIN75_00855"
     CDS             2997..3365
                     /gene="rpsF"
                     /locus_tag="VIN75_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8972734.1"
                     /translation="MRHYEIVLLIHPDQSEQVPAMLERYKALVTNSGGKVVRLEDWGR
                     RQLAYTIQKLAKAHYICLNIECGKEALAELETGFRFNDAVLRHLTVLRDKADTAPSVM
                     MKAVEKEEARKSASSQEATA"
     gene            3398..3682
                     /gene="priB"
                     /locus_tag="VIN75_00860"
     CDS             3398..3682
                     /gene="priB"
                     /locus_tag="VIN75_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY8972735.1"
                     /translation="MNRLILSAELVERGSTRYTPAGLPALDLSLKHESEVLEDKQPRK
                     VVLTIRAVAIGEITRKVATLPLGQVAGYAGFLAPSRNAKGLVFHVTDVDA"
     gene            3737..4021
                     /gene="rpsR"
                     /locus_tag="VIN75_00865"
     CDS             3737..4021
                     /gene="rpsR"
                     /locus_tag="VIN75_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8972736.1"
                     /translation="MPPPKSRFSKDKRPKRNQQSLLFRRKRFCRFTVAKVDHIDYKDV
                     DTLRDFVGENGKITPARLTGTRAFYQRQLTVAIKRARFLALLPYSDQHKV"
     gene            4035..4487
                     /gene="rplI"
                     /locus_tag="VIN75_00870"
     CDS             4035..4487
                     /gene="rplI"
                     /locus_tag="VIN75_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8972737.1"
                     /translation="MQIILLEKVANLGNLGDVVKVKDGYARNFLIPTRMARRATATAI
                     KEFEDRRAELEKAAAEKLATAQAFGEKLAGKTVRISQKAGVDGRLFGSVTNADIAEAL
                     TKNGYEVAKSQVRLPNGPLKTVGEHAVSIAAHTDVVVDVTVQVVAEQD"
     gene            4652..6067
                     /gene="dnaB"
                     /locus_tag="VIN75_00875"
     CDS             4652..6067
                     /gene="dnaB"
                     /locus_tag="VIN75_00875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8972738.1"
                     /translation="MTTVFDSAPPNRGQARDDEIAKLRVPPQSIEAEQSVLGGLLLDN
                     GAWDRAADIITADDFYRFEHKLIFGSIGALINASKPADVITVYEHLQMAGKAEDSGGL
                     TYLNQLAQSVPSAANMRRYAEIVRERAILRKLIAASDEIATNAFNPQGRAVTQILDEA
                     EGKIFKIGEEGNRNKQGFHSMDQLAVQLIDRVTELAENGAEDVTGVRTGYYDFDRNTA
                     GLQPGDLIILAARPSMGKTAFALNIAENVSVNEGLPVVVFSMEMGASQLALRMVGSQG
                     RIDQQHLRTGRLDNDEWGRLTEAVERLSKSSIFIDETPGLTGPELRARARRQARQCGQ
                     LGLIVIDYLQLMTGSGNGENRATEISEISRGLKALAKELHCPVIALSQLNRSVESRPD
                     KRPMMSDLRESGAIEQDADIIMFIYRDEYYNKESKEPGVAEIIIAKQRNGPVGTTKLA
                     FLRQWTKFENLAPDYQGPGEY"
     gene            complement(6135..7541)
                     /locus_tag="VIN75_00880"
     CDS             complement(6135..7541)
                     /locus_tag="VIN75_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8972739.1"
                     /translation="MSSTQPVEFHGRVVILGFGAIGQAILPLVLRDVRMPPSRIEVVE
                     PTGDAAQILRGSGVAHAQLALDPQNYSAELGRRLSQGDILLNLSVDVASVALMRWCQQ
                     QGVLYLDACTEPWPGVHDDPARPLAERTNYRRREEVLAIQRAETSRTTALVTLGANPG
                     LVSFFVKRALLELAGPGSPTPGTRQEWAALARGLDVRAIHVSERDSQVADRRKQAGEF
                     VNTWSIEGFAGEGLQPAELGWGTHERHFPADGHRHVHGSQAAIYLGRPGLGTRVRSWA
                     PIAGPFTGYLVTHAESISIADHLTVGDPAAPDYRPTVHYAYHPCDDAVLSIRELEGRH
                     WRLQEQHRMLRDDILSGVDELGVLLMSGTHGAYWYGSQLSIDQARALAPMNNATTLQV
                     AAPLVAGLRWAIEHPQEGVLEPDDLPHAAMLDVAMPYLGDVVGVRTDWTPLQDAPRLF
                     DEHLDRSDPWQFANVRIP"
     gene            8109..8945
                     /locus_tag="VIN75_00885"
     CDS             8109..8945
                     /locus_tag="VIN75_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8972740.1"
                     /translation="MNDLLAPTHAPVADDEPLAPPLVPAVVRALALLERVAQQRQPMS
                     ASGLAADLGLPKSSVHGLCNTLLHHGYLKRSGESGGFSIGARVMGLAEAFIAGTSVAH
                     EFGAMWDAESHSADESVILSVLNGTDAVYVAVRNSRRPLGLAFNVGVRLPAWLSGSGK
                     AMLAYQPDTVLERLFPPGPLSTLTGRGPADTRALFDELAAIRRRGYSIDDEGVRAGVL
                     SFGAPAFDAGGHVVAGVAMCINKSTLDAEALERHRAALLDTARRLTRRIGGRPPRGYP
                     IQ"
     gene            9001..9615
                     /locus_tag="VIN75_00890"
     CDS             9001..9615
                     /locus_tag="VIN75_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8972741.1"
                     /translation="MTLSPDAGANRSPELTLDYRSAFELSPMACALTRDRIIVDCNLK
                     FLAIFRTQREAVVGRSFEILFPTPEEFERTGRRIATAVSSSETGGYADERVMRRPGDG
                     SLFWCHVWGHALDRSRPHAAAIWSLEDISATRPIAVDLTPREREIGALLVEGLTSKLV
                     ARRLGLSHRTVEIYRSRLLRKHGVDTATGLVRKLIADTPSNLAA"
     gene            9830..11026
                     /locus_tag="VIN75_00895"
     CDS             9830..11026
                     /locus_tag="VIN75_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8972742.1"
                     /translation="MTAKDLRAPRATARARWAACLRRWTRAAGVLLATTLAAALPPAR
                     AADIVVGQVAPLTGVLATTGQQMVLGGRVCFDWVNDHGGINGARIVTVVADDGYDVAR
                     TEQATRALVARPEVVALFGLAGTANVARLLDDRVLADAGVALVAPYTGGEALRTPFNP
                     WIFHVRASYAREAEHMVQQLDTLGIRRIALLYQNDGFGQSGKAGIEAALARRGQSLAA
                     AAPYERNTGDVDAAVRTIIAAHAQAVIMVAVNKPAAAFIKAYRQGGGGGQLFNVSVVD
                     PAELVRLAGIDNARGLGISQVVPYPYRDTLPVVREFQAALHRYAPGQAVNYTNFEEYL
                     GAKVLVEALRRAGPNPTRARVLAALESLHNFDLGGMTVSFGPHERVGSTFVEVTVIDG
                     DGRLLK"
     gene            complement(11049..11861)
                     /locus_tag="VIN75_00900"
     CDS             complement(11049..11861)
                     /locus_tag="VIN75_00900"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY8972743.1"
                     /translation="MTSLFLAQRTALVTGAAQGIGLAIAARLAAAGARVVLHDLPGSP
                     HADEALAALREAGATDACFIGADLRASDRIDALMDEALRRHGGIDILVNNAGIQATAP
                     IVDMAPQRWDDIVAVNLSAAFHTMRRALPGMLARGYGRVVNIASVHGLVASREKAPYV
                     AAKFGLVGLTRVAALECATAGTRASGGITANCIAPGWTETSLIEPQILQRARAVGGDR
                     EAGVRELLREKQPSQRMSLPSEIAELACMLCQPWAHNINGTTLPVDGGWTAQ"
     gene            12220..13713
                     /locus_tag="VIN75_00905"
     CDS             12220..13713
                     /locus_tag="VIN75_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY8972744.1"
                     /translation="MSSFVHWAASSLAAAAFAAQAASPLPSLNVDKTQISVSGLSSGG
                     FMANQLGYAYSSTFMGVGVFAGGPYMCAGHNNYTACMYNATISASQLSTMQADLTNWS
                     GTNDDALSNVARQKVFLWVGSSDTTVGPNPMAAVKTQYTNNGVTAANLAWNQQSGANH
                     TFPTDFAGSGDNACGTAASPYISNCSFDGAGTMLKQFYGTLNARNDSPAGANYIQFDQ
                     TPYTAGNAGMAATGWIYVPASCASGASCRLHVALHGCLQNYAQIGADFIQNTGYTRWA
                     DTNHIIVLFPQTQVDNGTHATADSGSLANPNGCWDWIGWYGTTFAQKAGVQMKAIKAM
                     VDAVSAGSTGGGSGGGALPPPTGVGTSNATANSMTIAWNAVSGASGYNVYRNGAKVNS
                     AAVTGTSYVDTGLAPATSYSWTVTDLDSGGVESAQSSAASGTTTGTAATCYTASNYAH
                     VQAGRAHQSGGYAYANGSNQNMGLWNLYVTTTLKQTGTNYYVIGTCP"
     gene            13882..15006
                     /locus_tag="VIN75_00910"
     CDS             13882..15006
                     /locus_tag="VIN75_00910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8972745.1"
                     /translation="MTESRQARLQRHRLQMTLLSVVNSLLQSVVVGLFAASGATTWPL
                     ATAFAVASLATTGLFAVAVARGWVLRLGDARLLLAQLAVNFVVQIAFLFAMPTLWIVF
                     LASTLITCSYAMVALTPRQFTWLWLAYGLTTGLALLAGRARFGSPAPTTFNIGILWLF
                     FFMGVRRLAVTGAQFSRLREQLSEKNKQLTMLLERNQELATHDELTGAFNRRHLMGVI
                     VDERERARRSDVAYSVAILDLDWFKAINDGHGHAGGDAVLRAFCDLARAELRASDLFA
                     RYGGEEFVLLMPATPTPEAARSVVERIRHAVAAHDWRVVLGEQAPAVTVSAGIATWRR
                     DESAEVVLARADAALYDAKHLGRNRSVIAGGVIDDELVPA"
     gene            15118..15249
                     /locus_tag="VIN75_00915"
     CDS             15118..15249
                     /locus_tag="VIN75_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972746.1"
                     /translation="MKSHLLDTLRLLRTDRDLQDALFCCACWLGFASVAIGSVILPA"
BASE COUNT         2478 a         5406 c         5235 g         2249 t
ORIGIN      
        1 ttgtcaaggt tgccttggcg gcgttagggc gtctgtccta cccgatgata ggcagatcct
       61 ccgtagaatc ccggtcaact gaaccataaa tcttccggac gtcctccaaa gccatggccc
      121 gcctcgccgc acaactcgaa caacacgcag gggcgcgtcc atggattgct gacggccgga
      181 gcccggtttt ccatcatcaa ggagtttttt catgtccaac cccgcactgg ccacccgact
      241 ggtcattcca ttcgaggagc tgaggaacac cgacgtcgac atcgtcggcg gcaagaacgc
      301 cagcctcggc gagatgatca gccagctggc caccacgggc gtccgcgtcc ccggcggctt
      361 cgccaccacg gcgcacgcgt tccgccagtt cctggagttc ggcggcctga cgcagcgcat
      421 cgccgcgcgc ctgaagtcgc tcgacaccga tgacgtgcgc gcgctggccg aggccggcag
      481 ccagatccgc cagtggatcg aggacgcgcc gttcccgccg gagctcgaga agggcgtgcg
      541 cgaggaattc gcgcgcctgt ccgccagcga cccgaacgcc tcgttcgcgg tgcgctcgtc
      601 ggccacggcc gaggacatgc ccgacgcgtc cttcgccggc cagcaggaga ccttcctgaa
      661 cgtcgtcggc atcgacgccg tgctgcagaa gatgcgcgag gtcttcgcct cgctgtacaa
      721 cgaccgcgcg atcagctacc gcgtgcacaa gggctacgcc gacgccgaag tcgcgctgtc
      781 ggccggcgtg cagcgcatgg tgcgctccga cctcggcgcc gcgggcgtca tgttcacgct
      841 ggacaccgag agcggcttca ccgacgtcgt cttcatcacg gccagctacg gcctgggcga
      901 gacggtggtg cagggcgccg tcaatcccga cgagttctac gtccacaagc cggcgctgac
      961 cgctggcaag ttcccgatca tccgccgcac gctgggctcc aagctgatcc gcatggagtt
     1021 cgcctcggcc gaggagaagg cccgggacgg caagctggtg cgcaccgtcg acaccccgcc
     1081 ggagcagcgc aaccgctact cgctgagcga cgccgacgtg gtcgagctcg cgaagtacgc
     1141 gatgatcatc gagaagcact acggccgccc gatggacatc gagtggggca aggacggcgg
     1201 cgacggcctg atctacatcc tccaggcgcg tccggagacg gtcaagagca acgccgcgag
     1261 ccaggccgag catcgctaca agctcaaggg ctcgggcacc gtgctggtgg aaggccgcgc
     1321 gatcggccag aagatcggca ccggccccgt gcgcgtgtgc cgcaacgtct cggagatgga
     1381 ccaggtgcag cccggcgacg tgctcgtggc cgacatgacg gaccccaact gggagccggt
     1441 gatgaagcgc gccggcgcca tcgtcaccaa ccgcggcggc cgcacctgcc acgcggccat
     1501 catcgcgcgc gagctgggca tcccggccgt ggtcggctgc ggcgacgcga ccgacgtgct
     1561 gcacgacggc gcgctggtca ccgtggtctg ctcggaaggc gacaccggct acgtctacga
     1621 cggcctgctc gagaccgaag tcagcgaggt ggtgcgcggc gagatgccct acagcgccat
     1681 caagatcatg atgaacgtgg gcaacccgca gctggcattc aacttctcgc agctgcccaa
     1741 ctccggcgtc ggcctggcgc gcctggagtt catcatcaac aacaacatcg gcgtccaccc
     1801 gaaggcgatc ctcgactacc ccaacatcga cgtcgacctg aagaaggccg tcgagtcggt
     1861 ggcgcgcggc cacgcgtcgc cgcgcgcgtt ctacgtcgac aagctcgccg agggcatcgc
     1921 caccatcgcc gcggccttct ggcccaagcc ggtcatcgtg cgcctgtcgg acttcaagtc
     1981 caacgagtac aagaagctga tcggcggctc gcgctacgag cccgacgagg agaacccgat
     2041 gctgggcttc cgcggcgcgt cgcgctacat cagcggcgag ttcagcgacg cctttgcgat
     2101 ggagtgcgag gcgctgcgcc gcgtgcgcca cgacatgggc ctgaccaacg tcgagatcat
     2161 ggtgccgttc gtgcgcaccg tgcgccaggc cgagcgcgtc gtgacgatgc tcgccgagcg
     2221 cggcctcaag cgcggcgtcg acggcctgcg cgtcatcatg atgtgcgagg tgcccagcaa
     2281 cgccatcctg gccgaccagt tcctggagca cttcgacggc atgtccatcg gctccaacga
     2341 cctgacgcag ctgaccctgg gcctggaccg cgactccggc ctggagcagc tcgccgagga
     2401 cttcgacgag cgcgacccgg ccgtcaaggc gatgatctcg cgcgccatca acgcctgccg
     2461 cgccaccggc aagtacgtcg gcatctgcgg ccagggcccc agcgaccacc cggacttcgc
     2521 ggcctggctg gccgacgagg gcatcgtgtc gatctcgctg aaccccgaca ccgtcgtcga
     2581 cacctggacg gcgctggcgg ccaggaagta aagcgtcgcc gaggggactg gcctctcctc
     2641 acgtcacgct gagccgcaga tcgaagccgc ggtggcagtc gtgacgggag gcgaaggcct
     2701 gaccccgaac gccagccagg atcgaggtca ggccttggcc tcgacatcgc gggtgccgaa
     2761 cagcttgcgg cgccttcgtg gcgcgatgac gaggagaggc cagacccctc cggccgcggg
     2821 ccgccctgag ctgtctccca gcgatcgatg cctccatgcg atggcgttgt gctagaatct
     2881 ttggctttcc ttgccgcacc cgtggctgaa gcgtcatttc cgacgcggcg gccttctagt
     2941 tgacgctgtc ggggcggctt cattccagcc tggctggaaa tccacaagga gtgttcatgc
     3001 gtcactatga aatcgttctg ctgatccacc cggatcaaag cgaacaggtt ccggccatgc
     3061 tggaacgcta caaggccctc gtcaccaact ccggtggcaa ggtggtccga ctggaagact
     3121 ggggccgccg ccagctggcc tacacgatcc agaagctcgc caaggctcac tacatctgcc
     3181 tgaacatcga gtgcggcaag gaagccctgg ccgaactgga aaccggtttc cgcttcaacg
     3241 atgccgtcct gcgccacctc acggtgctgc gcgacaaggc cgacaccgcc ccgtccgtca
     3301 tgatgaaggc cgtggagaag gaagaagccc gcaagtcggc atcgtcgcag gaggcgacgg
     3361 cctgaaggca aaccccgcgc accgccgctg agcagcgatg aatcggctga ttctctctgc
     3421 ggagctggtg gaacgcggtt cgacccggta caccccggcc ggacttccag cgctcgacct
     3481 gagcctgaag cacgaatccg aggtgctcga agacaagcag ccgcgcaagg tcgtcctgac
     3541 gatccgtgcg gtggcgatcg gcgagatcac gcgcaaggtc gcgacacttc ccctgggaca
     3601 ggtcgccggc tacgccggat tcctcgcccc ctcgcgaaac gcgaagggcc ttgtcttcca
     3661 tgtcaccgac gtcgacgcct gacagcacgt cccccacgcc attcattcgg aacccatttc
     3721 tcgagaggtc atcaccatgc ccccgccgaa gtctcgtttt tccaaggaca agcgtccgaa
     3781 gcgcaaccag caatcgctgc tgttccgccg caagcgcttc tgccgcttca ccgtcgccaa
     3841 ggtcgatcac atcgattaca aggatgtcga caccctgcgc gatttcgtgg gtgagaacgg
     3901 caagatcacg ccggcccgcc tgaccggcac gcgcgcgttc taccagcgcc agctgacggt
     3961 cgccatcaag cgcgcccgct tcctggccct gctgccgtac agcgatcaac acaaggtctg
     4021 aggagaaacg acccatgcaa atcatcctgc tggaaaaggt cgccaacctc ggtaacctgg
     4081 gcgacgtcgt caaggtcaag gacggttacg cgcgcaactt cctgatcccg acccgcatgg
     4141 cgcgccgcgc caccgcgacc gcgatcaagg aattcgaaga ccgccgcgcc gagctggaaa
     4201 aggctgccgc cgagaagctg gccaccgcgc aagcgttcgg cgagaagctg gccggcaaga
     4261 ccgtgcgcat ttcgcagaag gccggcgtcg acggccgcct gttcggctcg gtcaccaacg
     4321 ccgacatcgc cgaagcgctg accaagaacg gctacgaagt cgccaagtcg caagtgcgcc
     4381 tgcccaacgg tccgctcaag accgtcggcg agcacgccgt ctcgatcgcc gcccacaccg
     4441 acgtcgtcgt cgacgtgacg gtgcaggtcg tcgccgagca agactgagcg tccagcgggc
     4501 ctcgtgcccg cttgcagcct cgaaagggcc gactcgcgtc ggcccttttt cattggcgac
     4561 acgaaaaagc ggcttgtccc accaagctcc ccaggctgtc cacaagcttg tccacagcgc
     4621 ttgtccgaga cgcctttaag atcgacgaca catgacgaca gtctttgact ccgccccgcc
     4681 caaccgcggc caggcccgcg acgacgagat cgccaagctg cgggtcccgc cgcaatccat
     4741 cgaggccgag cagagcgtgc tcggtggcct gctgctggac aacggcgcct gggaccgcgc
     4801 ggccgacatc atcacggccg atgacttcta ccgcttcgag cacaagctga tcttcggctc
     4861 catcggcgca ctgatcaacg ccagcaagcc cgccgacgtc atcaccgtct acgagcacct
     4921 gcagatggct ggcaaggccg aggactcggg cggcctgacc tacctgaacc agctcgcgca
     4981 gagcgtgccg agcgcggcca acatgcgccg ctacgccgag atcgtccgcg agcgcgcgat
     5041 cctgcgcaag ctgatcgccg cctccgacga gatcgccacc aacgcgttca atccgcaggg
     5101 ccgcgcggtc acgcagatcc tggacgaggc cgagggcaag atcttcaaga tcggcgagga
     5161 aggcaaccgc aacaagcagg gcttccacag catggaccag ctggcggtgc agctgatcga
     5221 ccgcgtgacc gaactggccg agaacggcgc cgaggacgtc accggcgtgc gcaccggcta
     5281 ctacgacttc gaccgcaaca ccgcgggcct gcagccgggc gacctgatca tcctggcggc
     5341 gcggccctcg atgggcaaga cggctttcgc tctgaacatc gccgagaacg tctcggtcaa
     5401 cgagggcctg ccggtcgtcg tgttctcgat ggaaatgggc gcgtcccagc tcgcgctgcg
     5461 gatggtcggc tcgcagggcc gcatcgacca gcagcacctg cgcaccggcc gcctcgacaa
     5521 cgacgagtgg ggccggctga cggaagccgt ggagcgcctg tccaagtcgt cgatcttcat
     5581 cgacgagacg ccgggcctga ccgggccgga gctgcgcgcc cgcgcgcgcc gccaggctcg
     5641 ccagtgcggc cagctgggcc tgatcgtgat cgactacctg cagctgatga cgggctcggg
     5701 caacggcgag aaccgcgcca ccgagatctc ggagatctcg cggggcctga aggcgctggc
     5761 caaggaactg cactgcccgg tgatcgcgct gtcgcagctg aaccgaagcg tcgagtcgcg
     5821 gcccgacaag cgccccatga tgtccgacct gcgcgaatcc ggcgccatcg agcaggacgc
     5881 cgacatcatc atgttcatct accgcgacga gtactacaac aaggaatcca aggagccggg
     5941 cgtcgccgag atcatcatcg ccaagcagcg taacggcccg gtcggcacga ccaagctggc
     6001 gttcctgcgg cagtggacga agttcgagaa cctcgcgccg gactaccaag ggcccggaga
     6061 gtactgagcg tctttcctgg catttgctcc aaccctgggc agaacgtcct gacacatccc
     6121 gccgacacgc ggcatcaagg gatgcgaacg ttggcaaact gccagggatc gcttcgatcc
     6181 agatgctcgt cgaagagccg cggggcgtcc tgcagaggcg tccagtcggt gcgaacgccg
     6241 acgacgtcgc cgaggtaggg catggcgaca tcgagcatgg ccgcgtgcgg gagatcgtcc
     6301 ggttcgagga cgccctcctg cgggtgctcg attgcccagc gcaggccggc cacgagtggc
     6361 gcggcgacct gcagggtcgt cgcgttgttc attggagcga gcgcgcgcgc ctggtcgatc
     6421 gacaactgcg acccatacca gtacgcgccg tgcgttccgc tcatcagcag cacgcccagc
     6481 tcgtcgacgc cggagaggat gtcgtcccgc agcattcgat gctgttcctg cagccgccaa
     6541 tggcgaccct cgagttcgcg gatcgagagg acggcatcgt cgcaggggtg gtaggcgtag
     6601 tgcacggtcg gacggtagtc gggcgccgcc gggtcgccaa ccgtcaggtg gtcggcgatg
     6661 gagatcgatt cggcgtgggt gaccagatag ccggtgaacg ggccggcaat gggcgcccaa
     6721 ctgcgcacgc gcgtcccgag accaggtctt cccaggtaga tggccgcttg cgatccgtgg
     6781 acgtgtcggt ggccgtcggc ggggaagtgg cgctcgtgcg ttccccatcc cagctcggcc
     6841 ggttgcaggc cctcgccggc gaagccttcg atcgaccagg tgttgacgaa ctcgcccgct
     6901 tgcttgcggc gatccgccac ttggctgtcg cgctcggaaa catggatggc gcgcacgtcc
     6961 agcccgcgcg cgagggcggc ccattcctgg cgcgtcccgg gcgtcggcga tcccgggcct
     7021 gccagctcca gcaaggccct cttcacgaag aacgagacaa gcccgggatt ggcgcccagc
     7081 gtgaccaagg cggtcgtgcg ggacgtttcc gcgcgctgga ttgccaggac ttcctcgcgc
     7141 cgccggtaat tcgtgcgttc ggcgagcggt cgagccggat cgtcgtgcac gccgggccaa
     7201 ggctccgtgc aggcatccag gtagagcacg ccctgctgtt gacaccagcg catgagggcg
     7261 acgctggcga cgtcgaccga caggttgagc aggatgtccc cttgcgacag ccggcgcccg
     7321 agctcggcgc tgtagttctg cgggtcgagc gcgagctggg catgcgccac gcccgatcct
     7381 cggaggatct gcgccgcgtc gccggtgggc tcgaccacct cgatgcgcga ggggggcatg
     7441 cgcacgtcgc gcaacacgag cgggaggatg gcctggccga tggcgccgaa cccgaggatg
     7501 acgacacggc cgtggaattc gacgggttgc gtgctgctca tgcgcatgtg aagcaaagac
     7561 ggtacccggg tccgcgccgt ccattgcagc cgctcgtgcc gcggcgcttg cggcgaggtg
     7621 agatcacggc cacgggcgag ccttgcgatc gatccgcggt cgttgattcg cctgcgtgcc
     7681 agcgctcgtg acctccgcgc gcctttgccc gtcgaacaga tcgaagcggc ccggacactt
     7741 cgcgagaggg cgcagcagct gctccgcgaa gccctgaacg agcaggcgac ggttgcccag
     7801 cgctgcggcg actccgggtg aagccggaca cgtgtcgatt gcgaaccggc gtggaaccag
     7861 gcggcccgcg ttgccggcag cgggcgtgag tccgatggca gcgtgatcgc gacaggcgcg
     7921 cgaaccttcg cgcccgactg gcagtgaccc gccgatcccg ctgcgcggtc gccttcgggc
     7981 gctgcgcacc acccccgtac tttcccgcga acggccgcac gaatgcccca ttcgggtgcc
     8041 gcgccgcgta gactgtctat cctgctggac gacgttcagc gggctgaaca accacatcga
     8101 ggaagcgcgt gaacgacctg ctggcgccca cccatgcccc tgttgccgat gacgagcccc
     8161 tggcgccgcc gctggtgccg gccgtcgtgc gcgcgttggc gctgctggag cgcgtggcgc
     8221 agcagcgcca gccgatgtcg gccagcgggc tggctgccga cctcggcctg cccaagagct
     8281 cggtgcacgg gttgtgcaac acgctgctgc accacggcta cctcaagcgc agcggcgaga
     8341 gcggcggctt ctcgatcggc gcgcgcgtga tggggctggc cgaggccttc atcgccggca
     8401 ccagcgtcgc ccacgagttc ggcgcgatgt gggatgccga gagccattcg gccgacgagt
     8461 ccgtcatcct gtccgtgctc aacggcaccg atgccgtcta cgtggcggtg cgcaacagcc
     8521 ggcggccgct ggggctcgcg ttcaacgtgg gcgtgcgctt gccggcctgg ctgtcgggca
     8581 gcggcaaggc gatgctcgcg taccagcccg acacggtgct cgagcggctg ttcccgccgg
     8641 gcccgctgtc cacgctcacg ggccgcggcc ctgccgatac gcgcgcgctg ttcgacgagc
     8701 tggcggcgat ccggcgacgc gggtacagca tcgacgacga aggcgtgcgc gcgggcgtgc
     8761 tgtcgttcgg tgctccggcc ttcgacgccg gcggccacgt ggtggcgggc gtggcgatgt
     8821 gcatcaacaa gtccacgctc gacgccgagg ccctcgaacg ccatcgcgcg gcgctgctgg
     8881 acaccgcgcg ccgcctgacg cggcgcatcg gcggccgccc gccgcgcggc tacccgatcc
     8941 aatgaatcga caaggcggtg ccggaatgtg ccggacgccg ctcgcatttg gaggcaaagc
     9001 atgaccctgt cccctgacgc cggcgcgaac cgttcccccg agctcacgct cgactaccgc
     9061 agcgccttcg agctctcgcc gatggcctgc gcgcttacgc gcgaccgcat catcgtcgac
     9121 tgcaacctga agttcctcgc catcttccgg acgcagcgcg aggcggtggt ggggcgctcg
     9181 ttcgagatcc tgtttccgac gcccgaggag ttcgagcgca ccggccgccg catcgccacg
     9241 gccgtcagca gctcggagac gggcggctac gccgacgagc gcgtcatgcg gcgccctggc
     9301 gacggcagcc tgttctggtg ccatgtctgg ggccacgcgc tcgatcgctc gcgtccgcac
     9361 gcggcggcga tctggtcgct ggaggacatc tcggccacgc ggccgatcgc ggtcgacctg
     9421 acgccgcgcg agcgcgagat cggcgcgctg ctggtcgagg gcctgaccag caagctcgtc
     9481 gcgcgccgcc tgggcctgag ccaccgcacg gtggagatct accgcagccg cctgctgcgc
     9541 aagcacggcg tcgacaccgc caccggcctg gtgcgcaagc tgatcgccga cacgccctcg
     9601 aacctcgcgg cctgagcgcg cccgcggcgt gccggtcagc gattaccccg atgtagtcgc
     9661 acgactgtcg ccggcgtccg tccggttcca gactgcgggt gcggtcgccg ggtgatccag
     9721 gacggactcc tcgtcgaatc gttgggacgc gcaaggcggc cgtcgagcgg cctgcgcgcg
     9781 agaaggacga tgccggagcc gcgagcgcgc gcccggcgag gagacatcga tgacggcgaa
     9841 agatcttcgc gcgccccggg ccacggcccg ggcgcgatgg gccgcatgcc tgcggcgctg
     9901 gacacgcgcg gccggggtgc tgctggcaac gacgcttgct gcagcgctac cgcctgcgcg
     9961 cgccgccgac atcgtcgtcg gccaggtcgc gccgctgacg ggcgtgctgg cgacgaccgg
    10021 gcagcagatg gtgctgggcg ggcgcgtgtg cttcgactgg gtcaacgacc atggcggcat
    10081 caacggcgcg cgcatcgtca cggtggtggc ggacgacggc tacgacgtcg cgcgcaccga
    10141 gcaggcgacg cgcgcgctcg tcgcacggcc cgaggtcgtc gcgctgttcg gcctcgccgg
    10201 caccgccaac gtcgcgcgcc tgctcgacga ccgcgtgctc gcggacgccg gcgtcgcgct
    10261 ggtggcgccg tacaccggcg gcgaggcgct gcgcacgccg ttcaacccgt ggatcttcca
    10321 cgtccgcgcg agctatgcgc gcgaggccga gcacatggtg cagcagctgg acacgctggg
    10381 catccgccgc atcgcgctgc tgtaccagaa cgacggcttc gggcagtccg gcaaggcggg
    10441 catcgaggcc gcgctggcgc ggcgcgggca gtcgctggcg gccgcggcgc cttacgagcg
    10501 caacaccggc gacgtggacg cggcggtgcg cacgatcatc gcggcgcacg cgcaggccgt
    10561 catcatggtg gcggtcaaca agccggccgc ggccttcatc aaggcctatc gccagggcgg
    10621 cgggggcggg cagctgttca acgtgtcggt ggtcgatccg gccgagctcg tgcggctcgc
    10681 gggcatcgac aacgcgcgcg gcctgggcat cagccaggtc gtgccttatc cgtatcgcga
    10741 cacgttgccc gtcgtgcgcg agttccaggc ggcgctgcac cgctacgcgc cgggacaggc
    10801 ggtcaactac acgaacttcg aggagtacct cggcgccaag gtgctggtgg aggcgttgcg
    10861 ccgcgccggc ccgaacccga cccgcgcgcg cgtgctcgcg gcgctggagt cgctgcacaa
    10921 cttcgacctg ggcggcatga ccgtcagctt cgggccgcac gagcgcgtgg gctcgacctt
    10981 cgtcgaggtg accgtgatcg acggcgacgg gcgcctgctc aagtgaggcg ggcggcgcgc
    11041 gccgcgcctc actgcgccgt ccagccgccg tccacgggca gcgtggtgcc gttgatgttg
    11101 tgcgcccagg gctggcacag catgcaggcc agctcggcga tctcggacgg cagtgacatg
    11161 cgctggctcg gctgcttctc gcgcagcagc tcgcgcacgc cggcctcgcg gtcgccgccg
    11221 accgcccgcg cgcgctgcag gatctgcggc tcgatcaggc tcgtctcggt ccagcccggc
    11281 gcgatgcagt tggcggtgat gccgccgctc gcgcgcgtgc ccgcggtcgc gcactcgagc
    11341 gcggccacgc gcgtgaggcc gaccaggccg aacttggcgg ccacgtaggg cgccttctcg
    11401 cgcgaggcca cgaggccgtg caccgaggcg atgttgacga cgcgtccgta gccgcgcgcg
    11461 agcatgccgg gcagggcgcg ccgcatcgtg tggaaggccg ccgacaggtt gacggcgacg
    11521 atgtcgtccc agcgctgcgg cgccatgtcg acgatgggcg cggtggcctg gatgccggcg
    11581 ttgttgacca ggatgtcgat gccgccgtgc cggcgcagcg cctcgtccat cagcgcgtcg
    11641 atgcgatcgc tcgcgcgcag gtcggcgccg atgaagcacg cgtcggtcgc gcctgcctcg
    11701 cgcaacgccg cgagcgcttc gtccgcgtgc ggcgagccgg gcaggtcgtg cagcacgacg
    11761 cgcgcgccgg cggcggccag gcgcgcggcg atcgcgaggc cgatgccttg cgcggcgccg
    11821 gtcacgagcg cggtgcgctg ggcaaggaag agggatgtca tggggtgtga tccttcgtgg
    11881 aatgcggggg gagtcgcggg tgcgcgacgc gaacccggcc agcgtaattc gcgcccacga
    11941 cggcccgcaa gccgcgtgat cccgtcagtg caaacccccg cacggcgacg gctccgaagg
    12001 cgcgggttca gtgggctgaa ccgcggcgct cggttcgttc aacgggctga atttcggcgc
    12061 acaaatcgcg ttgtcgaccg ggattccccg gtgtgcggcg catcgcaacc tgcgttcaat
    12121 gggctccccg cgtcggaccc agcgcaggac gcgatgcggc acgcatgccg gcaccgatgc
    12181 cggcaacctt cccttggcac gacaacatgg agaccatcga tgtcctcgtt cgttcactgg
    12241 gccgcatcct ccctcgcggc cgccgcgttc gccgcgcagg cggcctcgcc cctgccgtcg
    12301 ctcaacgtcg acaagacgca gatcagcgtg tccggcctgt cctccggcgg gttcatggcc
    12361 aaccagctcg gctacgcgta ctcgtccacc ttcatggggg tgggcgtgtt cgccggcggc
    12421 ccgtacatgt gcgcgggcca caacaactac accgcgtgca tgtacaacgc gaccatcagc
    12481 gcgtcgcagc tgagcacgat gcaggctgac ctgaccaact ggagcggcac gaacgacgat
    12541 gcgctgtcca acgtcgcgcg ccagaaggtc ttcctgtggg tcggcagctc cgacaccacc
    12601 gtcggcccga acccgatggc cgccgtgaag acccagtaca ccaacaacgg cgtcaccgcg
    12661 gccaacctcg cgtggaacca gcagtccggc gccaaccaca cgttccccac cgacttcgcg
    12721 ggctcgggcg acaacgcctg cggcacggcc gcctcgcctt acatcagcaa ctgcagcttc
    12781 gacggcgcgg gcacgatgct caagcagttc tatggcacgc tcaacgcgcg caacgactcg
    12841 ccggccggcg ccaactacat ccagttcgac cagacgccct acaccgcggg caacgcgggc
    12901 atggcggcaa ccggctggat ctacgtgccc gcgtcgtgcg ccagcggcgc cagctgccgc
    12961 ctgcacgtcg cgctgcacgg ctgcctgcag aactacgcgc agatcggcgc ggacttcatc
    13021 cagaacaccg gctacacgcg ctgggcggac accaaccaca tcatcgtgct gttcccgcag
    13081 acccaggtgg acaacggcac gcatgcgacc gcggacagcg gcagcctggc caacccgaac
    13141 ggctgctggg actggatcgg ctggtacggc acgacgttcg cgcagaaggc cggcgtgcag
    13201 atgaaggcga tcaaggcgat ggtcgacgcc gtctcggcgg gcagcaccgg cggcggcagt
    13261 ggcggcgggg cgctcccgcc gccgacgggc gtgggcacgt ccaacgccac ggcgaactcc
    13321 atgacgatcg cctggaacgc ggtgagcggc gcgagcggct acaacgtgta tcgcaacggc
    13381 gccaaggtga actcggccgc cgtcacgggc acgtcgtacg tcgataccgg cctggccccg
    13441 gcgacgagct attcgtggac ggtcaccgac ctggactcgg gcggcgtcga gagcgcccag
    13501 tcgtccgcgg ccagcggcac caccacgggc acggccgcga cctgctacac agccagcaac
    13561 tacgcccacg tgcaggccgg ccgcgcgcac cagtccggcg gctacgcgta cgcgaacggc
    13621 tccaaccaga acatgggcct gtggaacctg tacgtcacga ccacgctcaa gcagaccggc
    13681 acgaactact acgtcatcgg cacctgtccg tgagcggagc cgcgcatggg gcggtggacg
    13741 aacgttcagg agtagggaat tcgcgacccg gcctcaagtg gccgcgcggc cttccgaaag
    13801 aagcgccatg accttgcttc ccaccgccga gcgccgcgct gcctgcgcga cagtcgccct
    13861 gcgtcgtgcc cgggagcgcg cgtgaccgag tccaggcagg cccgcctgca acgccaccgg
    13921 ctgcagatga cgctgctgtc ggtggtgaac tcgctgctgc aatcggtcgt ggtcggcctg
    13981 ttcgcggcct cgggcgcgac gacgtggccg ctggccaccg cattcgcggt cgcgagcctc
    14041 gcgaccaccg gcctgttcgc agtcgcggtg gcgcgcggct gggtgctgcg gctgggcgac
    14101 gcgcggctgc tgctcgcgca gctggccgtc aacttcgtcg tccagatcgc gttcctgttc
    14161 gccatgccga cgctgtggat cgtgttcctg gcctcgacgt tgatcacctg cagctacgcg
    14221 atggtcgcgc tcacgccgcg ccagttcacc tggctgtggc tggcctatgg cctgaccacg
    14281 gggctcgcgt tgctcgcggg ccgcgcgcgc ttcggcagcc cggccccgac gacgttcaac
    14341 atcggcatcc tgtggctgtt cttcttcatg ggcgtgcggc gtctggccgt cacgggcgcg
    14401 cagttcagcc gcctgcgcga gcagctgtcg gagaagaaca agcagctgac catgctgctc
    14461 gagcgcaacc aggaactggc cacgcacgac gagctgaccg gggcgttcaa ccggcgccac
    14521 ctgatgggcg tgatcgtcga cgaacgcgag cgcgcgcgcc gttcggacgt cgcgtactcc
    14581 gtggcgatct tggacctcga ctggttcaag gcgatcaacg acggccacgg ccatgccggc
    14641 ggcgacgcgg tgctcagggc gttctgcgac ctcgcgcgcg ccgagctgcg cgcgagcgac
    14701 ctgttcgccc gctatggcgg cgaggagttc gtgctgctga tgccggcgac gccgaccccc
    14761 gaggccgcac gcagcgtcgt cgagcgcatc cgccacgcgg tcgccgcgca cgactggcgg
    14821 gtcgtgctgg gcgagcaggc gccggccgtg acggtgtccg cgggcatcgc cacctggcgc
    14881 cgcgacgagt cggccgaggt cgtgctggcg cgcgccgacg cggccctcta cgacgccaag
    14941 cacctgggcc gcaaccgcag cgtcatcgcc ggcggcgtga tcgacgacga gctcgttccg
    15001 gcctgaagtc cgctgtccgc ggcggcgtgg ggcgacttgc gtgaactggt tcacgcctgc
    15061 gcgcacggag cccgcgttgt cacggaatcg tcgcgcacgg tgcccaaact ggcgtccatg
    15121 aaatcgcatc tcctggacac gctgcgcctg ctgcgcaccg atcgcgacct gcaggacgcc
    15181 ctgttctgct gcgcctgctg gctcgggttc gcgagcgtgg ccatcggctc ggtgatcctg
    15241 cccgcctgac gcaccgcgcg ccggcgcgcg gcgtggccgt cagtggccgg cgcggcgcct
    15301 gatggcctcg atggccagcg cgcgcgtctg gtacgggtgc atgcagcgga agatgcagcg
    15361 gccgtggg
//
LOCUS       DATCRO010000033         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_67785, whole genome shotgun sequence.
ACCESSION   DATCRO010000033 DATCRO010000000
VERSION     DATCRO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67785"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1124
                     /locus_tag="VIN75_00920"
     CDS             279..1124
                     /locus_tag="VIN75_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY8972747.1"
                     /translation="MSALPAAAPARMPFDVEGWARWFQQREIPVLATTAAALEDLREI
                     EDDVDARRIADVVGHDPLMTLKVLIHASTQGSNRRLTDAETVVEALVLMGITPFFRAF
                     GPQPTIEDHLAGHAEALAGLARVMRRADRAARFAASFAIHRGDHDAVVLHEAAQLHDF
                     TEMLLWLHAPELALELQRRQLADPDLRSATVQRDVLHAELNDVQHVLMQVWHLPEMLV
                     RITDDRHAENSQVRNVLLAIRVARHSTEGWDNPALPDDVRDIAALLQLGLEPARQLLR
                     DIDGD"
     gene            complement(1284..2198)
                     /locus_tag="VIN75_00925"
     CDS             complement(1284..2198)
                     /locus_tag="VIN75_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8972748.1"
                     /translation="MIARVARILFLAWFALLGSLLALARADGWTPGLWVALYVLVLGH
                     PTLLAVEMLLARAVAARAGVTRARWRDLVRAVLREWLESTAVFGWRMPWRANAIEDHL
                     PKTSRGLRGVVFIHGYICNRGLWNPWMRRLLKLDRAFVAVSLEPVFASLDEYVPQIER
                     AVRRMEAATGLAPVIVAHSMGGLVARAWLRSRATHKRSDGTGEAARLITIGTPHRGTW
                     LAGLARTINARQMRVSSPWMTSLVEQEPATLQGQITSWWSECDQIVYPPPTAVYPGSE
                     AKQLRGVGHIALTAREEIWNEIRRRIDY"
     gene            complement(2308..3054)
                     /locus_tag="VIN75_00930"
     CDS             complement(2308..3054)
                     /locus_tag="VIN75_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8972749.1"
                     /translation="MNRLQGKRALITGGTTGIGLETARQFIAEGARVAVTGTNAETLE
                     QARIALGPDALVLRVDAAGVDAQAGLARTVGEAFGGIDAVFVNAGVADLRPLEAWDAA
                     GFDRSFDINLRGPFFLIQALLPWLAKPASIVLNTSINAHIGMPTSSVYAATKAGLLSL
                     ARTLSGELVGRGIRVNAVSPGPVTTPLYGKLGLSAGDLDKTAQAILSQVPAGRFGDPA
                     EIAKAVVFLASDEASFTVGAELIIDGGMGL"
     gene            3182..4084
                     /locus_tag="VIN75_00935"
     CDS             3182..4084
                     /locus_tag="VIN75_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972750.1"
                     /translation="MDRLRAFEVFVAVVSRQGFARAAEALDTSPANVTRYVAELEAHL
                     GTRLLNRHSRKLSLTESGEALYERARAILADVDEAVAVSSASTLAPQGRLRINAPQTF
                     GLLHLASRWPRFARRYPQVELDVSLIDRVVDVVEEGFDLAIRISRSGSTAHAARKLAT
                     SRNVVCASPDYLREHGTPSTPADLARHACIVYEYVGGEWSLLDADGRAQSVRVRAAMR
                     TNNGDTAAAAARAGMGIVWQPTFLVGPDLRAGRLVPLLPGWRVPDIDILAVYPSRRHL
                     SAKVRAMIDFLAEEFAGTPPWDRG"
     gene            4098..4829
                     /locus_tag="VIN75_00940"
     CDS             4098..4829
                     /locus_tag="VIN75_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972751.1"
                     /translation="MSDIATRILQSLSAVDAERRRRAAEPTLAASVAAVKAYQQARFE
                     RTHADLFDHPRYGRAARFFVDELYGPQDFAQRDAQFSRIVPALVRLFPDDIVETVEAL
                     AAVHALSERLDSTMAGHLHGAAPSRASYVRAWQATGEPPSRLRQLELVMRVGRSLDRH
                     TRSLVLRTSLKAMRGPARAAGLGALQSFLEAGFDAFGAMRGAREFLAAIHARETALVE
                     RLFEPAAVAAATGSPASDDPLAQLP"
     gene            4879..>5168
                     /locus_tag="VIN75_00945"
     CDS             4879..>5168
                     /locus_tag="VIN75_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8972752.1"
                     /translation="MNDVLASAATTSSRPWLTQYPPGVPGTVAVDTYASLVPLLEESW
                     TRHARRDAAACMGVRFSYGDIDERSRALGAWLQSKGVRRGDRVAIMMPNVPQ"
BASE COUNT          711 a         1883 c         1830 g          744 t
ORIGIN      
        1 ggatgtcgcg ctgccggaac catcgcaccc agccctcgac gtcgtgcggc gtgcgggcaa
       61 ggggggcggc gggcagggcg ctcatggcga gtctcggggt ccggtgacgg cgccccggcg
      121 gttcgggacg atcagttaca cggcatatcg gcatgcgccg ggcccgactt gagggcctgc
      181 cgatgtcccg cgcgacgcgg cgcgcgggcg tccgatatac cgagtaactg accgtcccgg
      241 accaacggga cgtggtcatc ggacttcgag atcccgccat gagcgccctg cccgccgccg
      301 cccccgcccg catgccattc gacgtcgagg ggtgggcacg ctggttccag cagcgcgaga
      361 tccccgtgct ggccaccacc gccgcggcgc tggaggacct gcgcgagatc gaggacgacg
      421 tcgacgcccg ccgcatcgcc gatgtggtcg ggcacgatcc gctgatgacc ctcaaggtgc
      481 tgatccacgc gtccacgcag gggtcgaacc ggcgcctgac cgatgccgag acggtggtcg
      541 aggcgctggt gctgatgggc atcacgccgt tcttccgtgc gttcggcccg cagccgacga
      601 tcgaggatca cctggccggc cacgccgagg cgctcgccgg actggcccgc gtgatgcgcc
      661 gcgccgatcg cgcggcccgc ttcgccgcca gcttcgcgat ccaccgcggc gaccacgacg
      721 cggtcgtgct gcacgaggcc gcgcagctgc acgacttcac cgagatgctg ctgtggctgc
      781 acgcccccga actcgcgctg gagcttcagc gccgccagct cgccgacccc gacctgcgct
      841 cggccaccgt gcagcgcgac gtgctgcacg ccgagctgaa cgacgtccag cacgtgctga
      901 tgcaggtatg gcacctcccc gagatgctcg tgcgcatcac cgacgaccgc cacgccgaga
      961 actcgcaggt ccgcaacgtc ctgctcgcga tccgcgtggc gcgccactcg acggaaggct
     1021 gggacaaccc ggcgttgccc gacgacgtgc gcgacatcgc cgcgctgctg caactgggac
     1081 tggagccggc gcggcagttg ctgcgcgaca tcgacgggga ctaggagtcg gcgcggcaga
     1141 agccgcctgc tgcgaacggc gataagcggg gccttttcag cggcggctcc tctcgcgtag
     1201 cgcccactac gagttcgtcg ccgccgctga aaagtccatg cttctcgccg ctctcgcgac
     1261 ggcgcctcct gccgcgcaca ctcctagtag tcgatcctgc gccggatctc gttccagatc
     1321 tcctcgcgcg cggtcagcgc gatgtggccg acgccgcgca gctgcttggc ctcgctgccc
     1381 gggtacaccg cggtcggcgg cgggtagacg atctggtcgc actcgctcca ccagctggtg
     1441 atctggcctt gcagcgtcgc cggctcctgc tcgacgagcg acgtcatcca cgggctgctg
     1501 acgcgcatct ggcgcgcgtt gatcgtgcgc gccaggcccg ccagccaggt gccgcgatgc
     1561 ggggtcccga tcgtgatgag gcgcgcggcc tcgccggtgc cgtcgctgcg cttgtgcgtc
     1621 gcgcgcgagc gcagccacgc gcgcgcgacg agcccgccca tgctgtgcgc cacgatcacc
     1681 ggcgcgaggc ccgtggccgc ctccatgcgg cgcaccgcgc gctcgatctg cggcacgtac
     1741 tcgtccaggc tcgcgaacac cggctccagg ctcaccgcga cgaacgcgcg gtcgagcttg
     1801 agcagccgcc gcatccacgg gttccacagg ccgcgattgc agatgtagcc atggatgaac
     1861 acgacgccgc gcagcccgcg cgaggtcttg ggcagatggt cctcgatggc gttcgcgcgc
     1921 cagggcatgc gccagccgaa cacggccgtc gactcgagcc attcgcgcag gacggcgcgc
     1981 accaggtcgc gccagcgcgc gcgcgtgacg ccagcgcgcg cggccacggc gcgggccagc
     2041 agcatctcga ccgccagcag cgtgggatgg cccagcacga gcacgtacag cgcgacccac
     2101 agtcccggcg tccacccgtc ggcccgggcc agagcgagca ggctgcccaa cagtgcgaac
     2161 caggccagga acaggatgcg ggcgacgcgc gcgatcatgc agcgagtatc acgcaggccc
     2221 gagggccctg cgcagccgga aagccttgag tgacgcggcg tcgcggagcc cgatgcactg
     2281 ccggggtatc cgccgccccg ccgccgctca gagccccatg ccgccgtcga tgatcagctc
     2341 ggcaccgacg gtgaaggacg cctcgtcgga ggccaggaac accaccgcct tggcgatctc
     2401 ggccgggtcg ccgaagcggc cggcgggcac ctgcgacagg atggcctgcg cggtcttgtc
     2461 gaggtcgccc gcgctcaggc ccagcttgcc gtacagcggc gtcgtcaccg ggcccgggct
     2521 gacagcgttg acgcggatgc cgcgtcccac gagctcgccc gacagcgtgc gcgccagcga
     2581 cagcagcccc gccttcgtcg ccgcgtacac gctgctggtg ggcatgccga tgtgtgcgtt
     2641 gatcgacgtg ttcaacacga tggacgccgg cttggcgagc cacggcagca aggcctggat
     2701 caggaagaac gggccgcgca ggttgatgtc gaagctgcgg tcgaagcctg cggcgtccca
     2761 cgcctcgagg ggccgcaggt cggcgacgcc cgcgttgacg aagacggcgt cgatgccgcc
     2821 gaaggcctcg ccgacggttc gcgcgagccc ggcctgggcg tcgacgccgg cggcgtccac
     2881 gcgcagcacg agggcgtccg ggccgagggc gatgcgagcc tgctcgagcg tctcggcgtt
     2941 ggtgccggtg acggccacgc gggcgccttc ggcgatgaac tggcgggccg tctccaggcc
     3001 gatgcccgtg gtgccgccgg tgatgagggc gcgcttgcct tgcagtctgt tcatgatgtg
     3061 ctccgttgcg ttgagggggg tcggccgagg ccggtgactg cactttgatc ttcatgaagc
     3121 cgtcaatcca ggcactgaaa gtgcacaatg cgttcagctt ttatgaacta tcggcggcgg
     3181 catggatcgg ttgcgtgcgt tcgaggtgtt cgtcgcggtg gtgtcgcgcc agggcttcgc
     3241 cagggccgcc gaggcgctgg acacctcgcc cgcgaacgtc acgcgttacg tcgcggagct
     3301 cgaggcccac ctgggcacgc gactgctgaa ccggcactcg cgcaagttgt cgctgaccga
     3361 gagcggcgag gcgctgtacg agcgggcgcg ggcgatcctg gccgacgtcg acgaggcggt
     3421 ggccgtcagc agcgcgtcga cgctggcacc gcagggccgg ttgcgcatca acgcgccgca
     3481 gaccttcggg ctgctgcacc tggcctcgcg ctggccgcgc tttgcccgcc gctacccgca
     3541 ggtcgagctg gacgtgtcgc tgatcgaccg cgtcgtggac gtcgtggagg agggcttcga
     3601 cctcgcgatc cgcatctctc gttcgggctc gacggcgcat gcggcgcgca agctggcgac
     3661 ctcgcgcaac gtggtctgcg catcgcccga ctacctgcgc gagcacggca cgccctctac
     3721 accggccgat ctcgcgcgcc atgcctgcat cgtctacgag tacgtcggcg gcgagtggtc
     3781 tttgctggac gccgatgggc gcgcgcagtc cgtgcgcgtg cgcgccgcga tgcggacgaa
     3841 caacggcgac accgccgccg ccgcggcgcg cgcgggcatg ggcatcgtgt ggcagccgac
     3901 cttcctcgtc ggccccgacc tgcgcgccgg ccggctggtg cccctgctgc ccggctggcg
     3961 cgtccccgac atcgacatcc tggccgtcta cccgagccgc cgtcacctca gcgccaaggt
     4021 gcgcgcgatg atcgacttcc tggccgagga gttcgccggc acgcccccgt gggaccgcgg
     4081 ctgataacct cgacgccatg agcgacatcg ccacccgcat cctgcagtcg ctgtccgcgg
     4141 tcgacgccga gcgacgccgg cgcgccgccg agcccacgtt ggccgccagc gtcgcggccg
     4201 tcaaggcgta ccagcaggcg cgcttcgagc gcacgcacgc cgacctgttc gaccacccgc
     4261 gctacggccg cgccgcgcgg ttcttcgtcg acgagctgta cggcccgcag gacttcgcgc
     4321 agcgcgacgc gcagttcagc cgcatcgtgc ccgccctcgt gcggctgttc cccgacgaca
     4381 tcgtcgagac agtggaggcg ctggccgcgg tgcacgcatt gtcggagcgc ctggactcga
     4441 ccatggccgg ccacctgcac ggcgcggcgc cgtcgcgtgc gagctacgtg cgcgcctggc
     4501 aggcgacggg tgaaccgccg agccgcctgc gccaactgga gctcgtgatg cgcgtgggcc
     4561 ggtcgctcga tcgccatacc cgcagcctgg tgctgcgcac cagcctgaag gcgatgcgcg
     4621 gcccggcgcg cgcggctggc ctcggggcgc tgcagtcgtt cctggaggcc ggcttcgacg
     4681 cgttcggcgc gatgcgcggc gcgcgcgaat tcctggccgc gatccacgcg cgcgagacgg
     4741 cgctcgtcga gcgcctgttc gagcctgcgg ctgtcgcggc cgcgacaggc tcgccggcat
     4801 cggacgaccc gttggcccag ttgccatagc cggtttcccg gggtgcggcg gtgccccggt
     4861 ggatgaaaat cggcggtcat gaacgacgtt ctcgcatccg ctgccaccac ttcgtcgcgt
     4921 ccctggctga cgcagtaccc gcccggcgtt cccggcaccg tcgcggtcga cacctatgcg
     4981 tcgctggtgc cgctgctcga ggagtcgtgg accaggcatg ccaggcgcga cgcggccgcg
     5041 tgcatgggcg ttcgcttcag ctacggcgac atcgacgagc gctcgcgcgc gctgggcgcc
     5101 tggctgcagt ccaagggcgt ccggcgcggc gaccgcgtgg ccatcatgat gcccaacgtg
     5161 ccgcagta
//
LOCUS       DATCRO010000034        10683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_877906, whole genome shotgun sequence.
ACCESSION   DATCRO010000034 DATCRO010000000
VERSION     DATCRO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877906"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..2404)
                     /locus_tag="VIN75_00950"
     CDS             complement(56..2404)
                     /locus_tag="VIN75_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8972753.1"
                     /translation="MDVSPDTGAGGWPSPATRLARAIAPVLPFLVVAVLVLGLIWGVT
                     LRLLASEHEAADRAAFVSSREQFETYQAQVVRALREVDETLKVVKYDYEMRGGRTDLA
                     QLRDRNLLPPDRFFEVSVTDSRGAVITRTGHSDSAIAFDARTLQFLHQGDTLVVSPPR
                     RNPATGRWRMQFARRLDGAGEQFAGVIAVEVDSDYFVSGYDPVTLGDHGVLGLVGVDG
                     VACAMRSGETVTAGQSVDFAALVAPEPPGNGADAVTVSPWDGIPRYEKARQLYDFPLA
                     VVVGLSADEQLAASRRDARTYLWRASAASVVLILVVAILATMSRRLALTRERAAEAQV
                     EHAARIEYLAYHDGLTGLPNRSLFSKLLAQSIHEAHRFDHCLAVLFLDLDRFKQINDT
                     LGHEGGDQLLQEVARRLRACLRDSDAVARLGGDEFVVLLPQIEDGQYAAAVGQKILSA
                     VSHPFVLTGQEFRVSVSVGISTYPQDGRDDMTLMKNADVAMYQAKQTGKNNFQFYSDE
                     LNADSLERMNLEAGLRRALAHDEFELEYQAKHDIRGGGITGVEALLRWRHPDLGTVAP
                     MQFIPIAEETGLIVSIGKWVFDTACRQYAAWLAQGLQPVTIAINLTARQFTDARLVGD
                     LAHALEAAGMPAERLELEISESLLTRDVAGSRAILAQLKALGVRIAVDDFGLGYLSLA
                     ALRQLPLDAIKIDRSFIRGAVVTADDQALTEAIIALGHNLSVTVVAQGVETREQADFL
                     RQNACDQFQGFFFDRPRPAAQVEALLRPRVLDNDGKRPGSDA"
     gene            2761..4620
                     /locus_tag="VIN75_00955"
     CDS             2761..4620
                     /locus_tag="VIN75_00955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8972754.1"
                     /translation="MNSLPSDLNPAPVLDGTQAPARLLEQADRLLYIDIRGAGALGQR
                     VLDATRDDPTLALRGEAHFHVAFSLLRTGQADRGVEHNELARASFAAHDDARGLLMCD
                     QFDALYLQIQGRLQEALELHLRILARGADVARRPVDLYICHNSRAITRKLLGQHDYML
                     LDFYEALAAAKSCDSPGPHINSLTNLGGSHTDLWNLGEAQKLSEQALDLAEAAGAWSS
                     FAVAVFNLAQIYDGLGLHERCVQMLARIERNKDRILPGVFARNTPLMAIAHLCAGDIE
                     GAGAWLDPGLESTAQVEPAIRTDHARAHATYLLATGRAAQAHDVVTAHLEAASKIELK
                     DPPYSRMRLLHVATDVCEALGDPACALRHLREAQRLYETLVDRSARAGFIATQVAHET
                     AIARDDRDRAREAQERAEVDRRRLATLNLALEERMSESQRLNAALQHKIAEAEALQEQ
                     LREQAVRDPLTGLYNRRFLDETSLARIQLARRQGSSIAIVLIDIDHFKQINDLYGHGR
                     GDEVLQAFARLLHERMRRSDIVCRFGGEEFLLLVDNAEPAALLELLDKALRQFRALRF
                     GVGDDLTDDCTFSAGVAWLGADGEDFESLVRVADLRMYRAKASGRARICHADG"
     gene            complement(4635..5633)
                     /locus_tag="VIN75_00960"
     CDS             complement(4635..5633)
                     /locus_tag="VIN75_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY8972755.1"
                     /translation="MSIEVRNVTKKYGDLAVCDNLSLDVPAGELVALLGPSGSGKTTL
                     LRIIAGLEEPDAGSVLFHGEDATRTGVRDRNVGFVFQHYALFGHMTIAENIAFGLRVR
                     PKATRPSDREIRARVADLLKLVQLDWLADRHPHQLSGGQRQRVALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDEMHVTSVFVTHDQEEAMEVADRVVVMNAGRIEQQ
                     GAPDHVYDHPATPFVLQFLGDVNLFARGAETDYVRPHEIDIVDAPAPGTWSVTVGQML
                     TVGPNTRIEFRRDDDGSFVDVELPRQDFTAARDRLGLMPGARVYLKPRRVTRFAAA"
     gene            complement(5630..6556)
                     /gene="cysW"
                     /locus_tag="VIN75_00965"
     CDS             complement(5630..6556)
                     /gene="cysW"
                     /locus_tag="VIN75_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY8972756.1"
                     /translation="MSAVIDSGERLAVADAPGPAAVRPVARARAHDAVSEPAWVRRTL
                     IGVALAFLTLFLFVPLVSVFAEALAKGVDVYWAALVEADAVSALKLTLIAAGVSVPLN
                     LVFGVAAAWAIAKFDFRGKSLLLTLIDLPFSVSPVIAGLIYMLVFGLQGWCGEWLRDH
                     DLKIVFAVPGIVLATVFVTFPFIARELIPLMQAQGQEQEEAARVLGASGWQILWRVTL
                     PNVRWALLYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHIEILYNDYQFAAAFAV
                     ASLLAGLALVTLVLKYIVERRAHAAFDLEKPE"
     gene            complement(6558..7391)
                     /gene="cysT"
                     /locus_tag="VIN75_00970"
     CDS             complement(6558..7391)
                     /gene="cysT"
                     /locus_tag="VIN75_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY8972757.1"
                     /translation="MSTRALLLRRHSVLPGFNLSLGFALLYLCFIVLVPLSAVFLKTF
                     TVSWGTFVATVTSPRVVASYRLTFGASLLAALVNGAFGLLVAWVLVRYRFAGKRIVDA
                     LVDLPFALPTAVAGIALTTLYASNGWIGRWLPFKVAYTPLGVFVALTFIGLPFVVRTV
                     QPVLEDLQRELEEAAATLGASRLQTFAYVIFPALMPALLTGFALAFARALGEYGSVIF
                     IAGNMPMVSEITSLLIITKLEQYDYTGATAIAVVMLVAAFALLLLINLLQAWARKRQG
                     R"
     gene            complement(7634..7924)
                     /locus_tag="VIN75_00975"
     CDS             complement(7634..7924)
                     /locus_tag="VIN75_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEY8972758.1"
                     /translation="MSVLVDEQSRSEMLNRLKRAEGQLRGIQRMIESGQPGIDVASQL
                     VAVRRALDSTYVHMTVSLVEQELGSRLGGDSRGRKQIGEVLGELTALLAKVR"
     gene            complement(8182..8775)
                     /locus_tag="VIN75_00980"
     CDS             complement(8182..8775)
                     /locus_tag="VIN75_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8972759.1"
                     /translation="MVHLVDDEAPVRDALTFLFQSHGLAVRAYASGPEFLDAADEAPL
                     AGCIVLDVRMEPMSGLQVHDALVARGSGVPVLFLTGHGDIPMAVEALKKGAFDFVEKP
                     SGDVALVDKVTRALAVDAARQAADSKGEETAARLASLSDREREVMQRVAAGKLNKVIA
                     DELCISVRTVEVHRARVFGKLGVRSAAEVATLLARGR"
     gene            complement(8811..>10683)
                     /locus_tag="VIN75_00985"
     CDS             complement(8811..>10683)
                     /locus_tag="VIN75_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8972760.1"
                     /translation="LELSDRADLESRQRQLITDSLSLESQITERVGEEQVQLNELARS
                     FDVAQPPDALGRQEAVADGLARLWQSVSWIDVDTRLVTVVPEGVSIVERPGISAHMSA
                     PLHDALGRPAGRLVVRYPPSMLLRQTVPWWLAHEYDVRLVDGYGQVVADPLKAGDREA
                     PPEQSHRHSLEPAMPDTYLELALHEPPRPWWLRLPIVMMAVFLMMIAVATALLRWQMN
                     EVLRTQTALRTEAAWRQAMEDSLTVGLRARDVEGRLVYVNRAFCDLVGFGPEELIGRA
                     TPMPYWPPDAIADSLSRHLRNMAGGAPRDGYEARWVRKDGQPIDVMLFEAPLVDASGR
                     HIGWMGSILDITERKRLEERERHQTDTMAHQARLTMLGEVASALAHQLNQPLTSITGY
                     AAGVKRLLERAGQPDARILDAMTRLGDQAAEAGRIVKRIREFLTRRSPQREAMDLAEA
                     AHRALALLGRELRRLQLHVEWVIPTGLPQVDADPVLIEQVVLNLVRNACDEMAGVPAS
                     QRRLRVSIARAGSGAPAGGDEGFLRLDVDDAGPGLRGRSVEQLVTPFYSTKPDGMGMG
                     LAICRSIVEAHHGAFDATASAFGGARFSFTLPLAAGGTPSELQQLGAQPDEQPEDLP"
BASE COUNT         1628 a         3832 c         3731 g         1492 t
ORIGIN      
        1 gcccgccagc gcgcgcaggc aggcgccccc accgcgcgcg gcatgcccgc ttgcatcagg
       61 catcggagcc gggccgcttg ccatcgttgt ccaggacacg cggccgcaac aatgcctcga
      121 cctgcgccgc gggccgcggc ctgtcgaaga agaagccctg gaactggtcg caggcgttct
      181 ggcgcaggaa atccgcctgt tcgcgggtct cgacgccctg ggcgacgacc gtcacgctca
      241 ggttgtgccc cagcgcgatg atggcctccg tcagcgcctg gtcgtcggcg gtgacgacgg
      301 cgccgcggat gaacgagcgg tcgatcttga tcgcgtccag cgggagctgc cgcaacgccg
      361 ccagggacag gtagccgagg ccgaagtcgt cgacggcgat gcgcacgccc agcgccttca
      421 actgggccag gatcgcccgg ctgccggcca cgtcacgcgt caacaggctc tccgagatct
      481 ccagttcgag ccgttccgcg ggcatgcccg cggcttccag cgcatgcgcc aggtccccca
      541 cgaggcgggc gtcggtgaac tgccgcgccg tcaggttgat ggcgatggtc acgggctgga
      601 gaccctgtgc cagccatgcc gcgtattgcc ggcaggccgt gtcgaacacc catttgccga
      661 tggacacgat caggccggtc tcctcggcga tcgggatgaa ctgcatcggg gcgaccgttc
      721 ccaggtcagg gtgccgccac cgcagcaggg cttccacgcc ggtgatgcca ccgccgcgga
      781 tgtcgtgctt ggcctggtat tcgagctcga actcgtcatg ggcgagcgcg cgccgcaggc
      841 cggcctccag attcattcgt tccagcgaat cggcattgag ctcgtccgaa tagaactgga
      901 agttgttctt gccggtctgc ttggcctggt acatcgcgac atcggcattc ttcatcaacg
      961 tcatgtcgtc gcggccgtcc tgcggatagg tgctgatgcc gacgctgacg ctgacgcgga
     1021 attcctggcc cgtcaacacg aaggggtgcg agacggcgga caggatcttc tggccgaccg
     1081 cggcggcgta ctgcccgtcc tcgatctgcg gcagcaggac gacgaactcg tcgccgccca
     1141 gccgggccac cgcatcgctg tcccgcaggc aggcccgcag gcggcgcgcg acctcctgca
     1201 gcagctggtc gcccccctcg tggccgagcg tgtcgttgat ctgcttgaaa cgatcgaggt
     1261 cgaggaacag cacggccagg cagtggtcga agcgatgcgc ctcgtggatc gactgcgcca
     1321 gcagcttgct gaacaggctg cggttgggca ggccggtcag gccgtcgtga taggcgaggt
     1381 actcgatgcg ggcggcgtgc tcgacctggg cctccgcggc gcgctcgcgg gtcagggcca
     1441 ggcgccggct catggtcgcc aggatggcca ccacgaggat caggaccacg ctggccgcgc
     1501 tcgcgcgcca gagataggtg cgcgcgtcgc gccgcgatgc ggccagctgt tcgtccgccg
     1561 acaggccgac gaccactgcc agcgggaagt cgtagagctg gcgcgccttc tcgtagcgcg
     1621 ggatgccgtc ccagggcgag acggtcacgg catcggcccc gttgccgggc ggctccggcg
     1681 ccacgagggc ggcgaagtcg accgactgcc ccgcagtgac cgtctcgccg ctgcgcatcg
     1741 cgcacgccac gccgtcgacc cccacgagcc cgagcacgcc gtggtcgccg agggtcaccg
     1801 ggtcgtagcc gctcacgaag tagtcggagt cgacctcgac cgcgatcacg ccggcgaact
     1861 gttcaccggc gccatcgagc cggcgggcga actgcatgcg ccatcggccg gtggccgggt
     1921 tgcggcgcgg cggactgacc acgagggtgt cgccctgatg caggaactgc aacgtgcggg
     1981 cgtcgaaggc gatcgccgag tcggaatgac cggtgcgcgt gatcacggcg ccccggctgt
     2041 cggtgacgct gacctcgaag aaccgatccg gcggcagcag gttccggtcc cgcagctggg
     2101 cgagatccgt ccggccgcct cgcatctcgt agtcgtactt gaccaccttc agggtctcgt
     2161 cgacttcacg caaggcccgc acgacctggg cctggtaggt ctcgaactgc tcgcggctgg
     2221 agacgaaggc ggctcgatcg gcggcttcat gctcggaggc caggagccgg agggtcacgc
     2281 cccagatgag gccgagcacc aggacggcga cgaccaggaa cggcaggacg ggcgcgatcg
     2341 cgcgcgcgag ccgggtggca ggtgacggcc acccgccggc gccggtgtcg gggctcacgt
     2401 ccatgcgggg cgccggggaa cggccgcgaa ggcgctgcga acgacccgcg gccggcccgg
     2461 ggcggggccg ggcggacgga tggccgcggt cgcaggcctc cagacaacca ggtgcggttt
     2521 ggcactcatc gaattgttga ccggttctct ctgcccggag attaccgcag agtgaagcgc
     2581 gattcatggg attcgccgtc ggccccaagc gacccggccg atcgcttccc ggcaaagcga
     2641 gaactatccg acggaatcag gatcaccctg ccgtcgattc ctcaagccgt ggctgcacca
     2701 tccgattcag gtactgatgc gccctcctcc cggcaggcgc cttcattgcc cgatgatccg
     2761 gtgaactcgc tgccgtccga cctcaacccc gcccccgtgc tcgacggcac gcaggccccg
     2821 gcgcgcctgc tcgagcaggc cgaccggctg ctctacatcg acatccgcgg cgccggcgcg
     2881 ctcggccagc gcgtgctgga tgccacccgg gacgacccga cgctcgcgct gcgcggcgag
     2941 gcgcatttcc acgtcgcgtt ctcgctgttg cgcaccggcc aggccgatcg cggcgtggag
     3001 cacaacgaac tcgcccgcgc ctccttcgcc gcgcacgacg atgcgcgcgg cctgctgatg
     3061 tgcgaccagt tcgacgcgct gtacctgcag atccagggcc ggctgcagga ggcgctcgag
     3121 ctgcacctgc gcatcctggc gcgcggcgcg gacgtcgcgc gccggccggt cgacctctac
     3181 atctgccaca actcgcgcgc catcacgcgc aagctgctgg gccagcacga ctacatgctg
     3241 ctggacttct acgaggcgct cgccgcggcc aagtcctgcg attcgcccgg cccgcacatc
     3301 aactcgctga ccaacctggg cggcagccac accgacctgt ggaacctggg cgaggcccag
     3361 aagctgtcgg agcaggcgct ggacctggcc gaggccgcgg gcgcatggtc gtcgttcgcg
     3421 gtggccgtgt tcaacctcgc gcagatctac gacggcctgg ggctgcacga acgctgcgtc
     3481 cagatgctgg cgcgcatcga gcgcaacaag gatcgcatcc tgcccggcgt gttcgcccgc
     3541 aacacgccgc tgatggcgat cgcccacctg tgcgcgggcg acatcgaggg cgccggtgcg
     3601 tggctggacc cgggcctcga gtcgaccgcg caggtcgagc ccgccatccg caccgaccac
     3661 gcccgcgccc acgccacgta cctgctggcc accggccgcg cggcgcaggc gcacgacgtg
     3721 gtgacggcgc atctggaggc cgcttccaag atcgagctga aggacccgcc ctactcgcgc
     3781 atgcgcctgc tgcacgtggc caccgacgtc tgcgaggcgc tgggcgaccc cgcctgcgcg
     3841 ctgcgccacc tgcgcgaggc acagcggctc tacgagacgc tggtcgaccg cagcgcgcgc
     3901 gccggcttca tcgccacgca ggtcgcgcac gagacggcca tcgcgcgcga cgaccgcgac
     3961 cgcgcccgcg aggcacagga gcgcgcggag gtggaccgcc gccggctggc gacgctgaac
     4021 ctcgcgctgg aggagcgcat gagcgagtcg cagcgcctga atgcggcgct gcagcacaag
     4081 atcgccgagg ccgaggcgct gcaggagcag ctgcgcgagc aggcggtgcg cgaccccttg
     4141 accggcctgt acaaccggcg cttcctcgac gagaccagcc tcgcgcgcat ccagctcgcg
     4201 cgccgccagg gctcgtcgat cgccatcgtg ctgatcgaca tcgaccactt caagcagatc
     4261 aacgacctgt acggccacgg ccgcggcgac gaggtgctgc aggcgttcgc ccggttgctg
     4321 cacgagcgca tgcgccgcag cgacatcgtc tgtcgcttcg gcggcgagga gttcctgctg
     4381 ctggtggaca acgccgaacc tgccgccctg ctcgagctgc tggacaaggc cctgcgccag
     4441 ttccgcgcgc tgcgcttcgg cgtcggcgac gacctcaccg acgactgcac cttctcggcc
     4501 ggcgtcgcct ggctgggcgc cgacggcgag gacttcgaat cgctggtgcg cgtggccgac
     4561 ctgcgcatgt accgcgccaa ggcctcgggc cgcgcgcgca tctgccacgc ggacggctga
     4621 ggcctgccgc tctttcaggc cgccgcgaag cgcgtcactc gccgcggctt gaggtacacg
     4681 cgtgccccgg gcatcaggcc caggcggtcg cgtgcggccg tgaagtcctg gcgcggcagc
     4741 tccacgtcga cgaagctgcc atcgtcgtcg cgcctgaact cgatgcgcgt gttcgggccc
     4801 accgtgagca tctggcccac cgtgacggac cacgtgcccg gcgcgggcgc gtcgacgatg
     4861 tcgatctcgt gcgggcgcac gtagtccgtt tccgcaccgc gcgcgaacag gttcacgtcg
     4921 ccgaggaact gcagcacgaa cggcgtggcc gggtggtcgt agacgtggtc cggcgcccct
     4981 tgctgctcga tcctgccggc attcatgacg accacgcggt cggccacctc catcgcctct
     5041 tcctggtcgt gcgtgacgaa cacgctggtg acgtgcatct cgtcgtgcag gcggcgcagc
     5101 cagcggcgca gctccttgcg caccttggcg tccagcgcgc cgaagggctc gtccagcagc
     5161 agcaccttgg gctcgaccgc cagcgcgcgg gcgagcgcga cgcgctgacg ctggccgccc
     5221 gagagctggt gcgggtggcg gtcggcgagc cagtccagct gcaccagctt gagcaggtcg
     5281 gcgacgcggg cgcggatctc gcggtcggac ggccgcgtcg ccttgggccg cacgcgcagg
     5341 ccgaacgcga tgttctcggc gatggtcatg tggccgaaca gcgcgtagtg ctggaagacg
     5401 aagcccacgt tgcgatcgcg cacgccggtg cgggtggcgt cctcgccatg gaacagcacg
     5461 ctgcccgcgt cgggctcctc gaggcccgcg atgatgcgca gcagcgtggt cttgcccgag
     5521 cccgacgggc ccagcagcgc gacgagctcg cccgcgggca cgtccaggct caggttgtcg
     5581 cagacggcga ggtcgccgta tttcttcgtg acgttgcgga cttcgatgct cattccggtt
     5641 tctccaggtc gaacgccgca tgcgcgcgcc gctcgacgat gtacttgagc accagcgtga
     5701 ccagcgccag gccggccagc agcgacgcga cggcgaaggc cgcggcgaac tggtagtcgt
     5761 tgtagaggat ctcgatgtgc agcggcagcg tgttggtctg gccgcggatg tggcccgaga
     5821 ccacgctgac cgcgccgaac tcgcccatcg cccgggcgtt gcacaggatc acgccgtaca
     5881 gcagcgccca gcggacgttg ggcagcgtga cgcgccacag gatctgccag cccgaggcgc
     5941 ccagcacgcg cgcggcctcc tcctgctcct ggccctgcgc ctgcatcagc gggatcagct
     6001 cgcgcgcgat gaacgggaac gtgacgaaca cggtggccag cacgatgccg ggcaccgcga
     6061 acacgatctt gaggtcgtgg tcgcgcagcc actcgccgca ccagccctgc agcccgaaca
     6121 ccagcatgta gatcaggccc gcgatcaccg gcgacaccga gaacggcagg tcgatcagcg
     6181 tcagcagcag gctcttgccg cggaagtcga acttcgcgat ggcccacgcg gcggccacgc
     6241 cgaacacgag gttcagcggc acggacacgc ccgcggcgat cagcgtgagc ttgagcgcgg
     6301 agacggcgtc ggcctcgacc agcgcggccc agtagacgtc cacgcccttg gccagcgctt
     6361 ccgcgaagac cgataccagc gggacgaaca ggaacagcgt gagaaaggcc agcgcgacgc
     6421 cgatcagcgt gcggcgcacc caggccggct cgctgacggc gtcatgcgcg cgcgcgcgcg
     6481 cgaccgggcg cacggccgcg ggcccgggcg cgtccgcgac ggccaggcgc tcgcccgaat
     6541 cgatcacggc gctcatctca gcgcccctgg cgcttgcgcg cccaggcctg cagcaggttg
     6601 atcagcagca gcagcgcgaa ggccgccacc agcatgacga ccgcgatcgc ggtggcgccc
     6661 gtgtagtcgt actgctcgag cttcgtgatg atcagcagcg acgtgatctc cgagaccatg
     6721 ggcatgttgc ccgcgatgaa gatcacggag ccgtactcgc cgagcgcgcg cgcgaacgcc
     6781 agcgcgaagc cggtcagcag cgccggcatc agcgccggga agatcacgta cgcgaacgtc
     6841 tgcagccgcg aggcgcccag cgtggcggcg gcctcttcca gctcgcgctg caggtcctcc
     6901 agcaccggct gcaccgtgcg cacgacgaac ggcaggccga tgaaggtcag cgcgacgaac
     6961 acgcccagcg gcgtgtacgc caccttgaac ggcagccacc tcccgatcca gccgttgctc
     7021 gcgtacagcg tggtcagcgc gatgcccgcc accgcggtcg gcagcgcgaa cggcaggtcg
     7081 acgagcgcgt cgacgatgcg cttgcccgcg aagcggtagc gcaccagcac ccaggccacc
     7141 agcagcccga aagcgccgtt gaccagcgcg gccagcaggg acgcgccgaa cgtgaggcga
     7201 tacgacgcga cgacgcgcgg cgacgtcacc gtcgcgacga acgtgcccca gctgaccgtg
     7261 aaggtcttga ggaacaccgc cgacagcggg acgaggacga tgaagcagag gtacagcaac
     7321 gcgaagccga gggacaggtt gaaccccggc aacacgctgt gccgccgcag cagcagggcg
     7381 cgcgtggaca tggtggaggc agcaggcgac gagaggcgat gagccgcgcc acgacccgga
     7441 cgggggccat ggtggcgcgt tcatccactc taggacgcac ggcggcaacc gccaacgaat
     7501 cgaagcgcgt ttccttattc gcgattcgtc ggacccatat tcgccaaacg gtgcagccgc
     7561 ttcgcgcacg gcaaagcgga cgttccgcga tggcgaagcg tcgacgcggc cccgacggcc
     7621 ggcggcctcg ccgtcagcgc accttggcca gcaatgcggt cagctcgccc agcacttcac
     7681 cgatctgctt gcgacctcgc gagtcgccgc ccagccggct gccgagctcc tgctcgacca
     7741 ggctgacggt catgtgcaca taggtgctgt ccagggcgcg gcggaccgcg accagctggc
     7801 tcgcgacgtc gatgccgggc tggccggact cgatcattcg ctggatacct cgcagctggc
     7861 cttcggcacg cttgaggcgg ttgagcattt cgctgcggct ttgttcatcg acaagcacgg
     7921 acattttttg atccctccaa cggcagcgct cctgacgggc cgccgcactg ccattgtggg
     7981 cttttcacga actttgcata ggtggtgcgg tacccggatg ccacgaaaac ctcgtaaata
     8041 tcgggggggc cgtcgggggg gagcgggttt gcacgacgcc ggcgccgggc cgccatccag
     8101 ggaaaacgcg gggaaaggca tcgataccac cgaccgtatc gagagatgct atcgaaccgg
     8161 gttgcgcctg tcagcgcgct gtcagcgtcc gcgcgccagc agcgtcgcga cctcggccgc
     8221 cgaacgaacg cccagcttgc cgaacacgcg cgcgcggtgc acttccaccg tgcgcacgga
     8281 gatgcacagt tcatcggcga tgaccttgtt cagcttgccc gcggccacgc gctgcatcac
     8341 ctcgcgctcg cggtccgaca ggctggcgag ccgcgccgcg gtctcctcgc ccttggagtc
     8401 ggcggcctgg cgcgccgcgt cgacggccag cgcgcgcgtg accttgtcga ccagcgcgac
     8461 gtcgcccgag ggcttctcga cgaagtcgaa cgcgcctttc ttgagcgcct ccacggccat
     8521 ggggatgtcg ccgtggcccg tgaggaacag caccggcacg ccgctgccgc gcgccaccag
     8581 cgcgtcatgc acctgcagac ccgacatcgg ctccatgcgc acgtccagca cgatgcagcc
     8641 ggccagcggc gcctcgtcgg ccgcatcgag gaactcgggg ccgctcgcgt acgcgcgcac
     8701 cgccaggccg tgcgactgga acaggaaggt cagcgcatcg cgcaccggcg cctcgtcgtc
     8761 gacgaggtgc accatcggtt cgggcgggcg ggcttggagc gtcgtggcgt tcatggcaga
     8821 tcctcgggtt gctcgtcggg ctgcgcgccg agctgctgca gctccgacgg cgtcccgccg
     8881 gcggccagcg gcagggtgaa ggagaaccgc gcgccgccga aggcgctggc ggtggcgtcg
     8941 aaggcgccgt ggtgcgcctc gacgatggag cggcagatcg ccaggcccat gcccatgccg
     9001 tcgggcttgg tcgaatagaa cggcgtgacc agctgctcga cgctgcgccc gcgcaggccg
     9061 ggcccggcgt cgtcgacgtc cagccgcagg aagccctcgt cgcccccggc cggcgcgccc
     9121 gagccggcgc gcgcgatgct cacgcgcagc cggcgttgcg aggccggcac gccggccatc
     9181 tcgtcgcacg cgttgcgcac gaggttgagc acgacctgct cgatcagcac cggatccgca
     9241 tcgacctgcg gcaggcccgt cgggatgacc cattcgacgt gcagctgcag ccgccgcagc
     9301 tcgcgtccga gcagcgcgag cgcgcgatgc gcggcctcgg ccaggtccat cgcctcgcgc
     9361 tgcgggctgc ggcgcgtcag gaactcgcgg atgcgcttga cgatgcggcc cgcctcggcg
     9421 gcctggtcgc ccaggcgcgt catcgcatcg aggatgcgcg cgtcgggctg gcccgcgcgt
     9481 tcgagcagcc gcttgacgcc cgcggcgtag ccggtgatgg aggtcagcgg ctggttcagc
     9541 tggtgcgcga gcgcggacgc cacctcgccc agcatcgtca gccgcgcctg gtgcgccatc
     9601 gtgtcggtct ggtgccgctc gcgctcctcg aggcgcttgc gctcggtgat gtccaggatc
     9661 gagcccatcc agccgatgtg gcggccgctc gcgtccacca gcggcgcctc gaacagcatg
     9721 acgtcgatcg gctggccgtc cttgcgcacc cagcgcgcct cgtagccgtc gcgcggcgcg
     9781 ccgccggcca tgttgcgcag gtggcgcgac aggctgtcgg cgatggcgtc gggcggccag
     9841 tagggcatcg gcgtcgcgcg gccgatcagt tcctccggac cgaagcccac caggtcgcag
     9901 aacgcgcggt tgacgtacac gagccggccc tcgacgtcgc gcgcgcgcag gcccacggtc
     9961 agcgagtcct ccatcgcctg ccgccaggcg gcctcggtgc gcaacgccgt ctgcgtgcgc
    10021 agcacctcgt tcatctgcca gcgcagcaat gccgtggcca cggcgatcat catcaggaac
    10081 acggccatca tcacgatggg caggcgcagc caccacggcc gcggcggctc gtgcagcgcg
    10141 agctccaggt aggtgtcggg catcgccggc tccaggctgt ggcggtgcga ctgctcgggg
    10201 ggagcctcgc ggtcgccggc cttgagcgga tcggccacca cctgcccgta gccgtcgacc
    10261 aggcgcacgt cgtactcgtg cgcgagccac cacggcacgg tctggcgcag cagcatcgag
    10321 ggcggatagc gcacgacgag cctgcccgcg ggccggccga gggcgtcgtg cagcggtgcg
    10381 ctcatgtgcg cgctgatgcc gggccgctcg acgatgctca cgccctcggg cacgacggtc
    10441 accaggcgcg tgtcgacgtc gatccagctc acgctctgcc acagccgcgc gaggccgtcg
    10501 gccaccgctt cctggcggcc cagcgcatcg ggcggctgcg ccacgtcgaa gctgcgcgcg
    10561 agctcgttga gctgcacctg ctcctcgccg acgcgctcgg tgatctggct ctcgagcgac
    10621 agcgagtccg tgatgagctg gcgctggcgc gactcgaggt ccgcgcggtc cgacagctcc
    10681 agc
//
LOCUS       DATCRO010000035         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_405377, whole genome shotgun sequence.
ACCESSION   DATCRO010000035 DATCRO010000000
VERSION     DATCRO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405377"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VIN75_00990"
     CDS             complement(<1..750)
                     /locus_tag="VIN75_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEY8972761.1"
                     /translation="MHSTRSSRRPVLLAVAVAAWLAVLLGGCTKQADPNARAGDLPVD
                     AEFAKKLQGQLLDARPGSVIEIPAGRWHLDRGLSLRVNGVTVRGAGMEQTILSFRNQV
                     AGPEGLLAQASDFTVEGLTIEDTKGDALKINSGDNITIRNVRVRWTDGPKTSNGAYGL
                     YPVKTNNVLIEGSQSFGAADAGIYVGQSRNVIVRNCRAEQNVAGIEIENTIDADVHDN
                     VATGNTGGILVFNMPNLSQPGHSTRVFHNKVD"
     gene            930..1613
                     /locus_tag="VIN75_00995"
     CDS             930..1613
                     /locus_tag="VIN75_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8972762.1"
                     /translation="MPRKPQASVDAPANGAAGVELDRKRDPTQTRALETCERILDAAA
                     NLLGEVGIERLSTNLICERAGLSPPALYRYYPNKYAVLAELGVRLMMSQNALLESWAT
                     PATMKLPAGKFEDRICRLFLDTLEMTRRATAGEWITRALRAVPSLAPVRLESHAHVTG
                     LLVAAFAVAWPAVPQSRVRLVSRLAVEVMYSAHELMFDDPALDAEEVGRVMSHMVTAQ
                     VMQLRRETR"
     gene            complement(1662..>2831)
                     /locus_tag="VIN75_01000"
     CDS             complement(1662..>2831)
                     /locus_tag="VIN75_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8972763.1"
                     /translation="PASLAAALRKAEAGKPLPWFHAATGVDDDQRLAVAQRSEATGYW
                     VVGEAAGAEALRGQWRTLAPFLALFGAAAMALVAGLYVLLRRWVGRPLAAVTSALDHV
                     AAGDLSQPVPEGGPDEIGAMLRSVEAMRIRFIDMLGDVRAAAGAVATASAQVAAGNLD
                     LSHRTEQAAARLERANATMDSVHGALRESAAESKDASGLASNAADAAHRGQAAMDDLA
                     ARMNHLSAGSARMSEIVGVIDGLAFQTNLLALNAAVEAARAGESGRGFGVVAGEVRNL
                     AQRCASAAKEIDGLLTASVAEIHGSHELTHVVGQRMAVIVGGVQAVHASLENIAHRIE
                     RQTSGLGDLANSVNLIDRMTQQNAALVEQSMAAAESLNSQAGRLVEIVGVFRLEA"
BASE COUNT          385 a         1022 c         1003 g          421 t
ORIGIN      
        1 gtcgaccttg ttgtggaaca cgcgcgtgct gtgccccggc tgcgacaggt tgggcatgtt
       61 gaacacgagg atgccgccgg tgttgccggt ggccacgttg tcgtgcacgt cggcgtcgat
      121 ggtgttctcg atctcgatgc cggccacgtt ctgctcggcg cggcagttgc gcacgatgac
      181 gttgcgcgac tgcccgacgt agatgccggc gtcggccgcg ccgaacgact gcgagccctc
      241 gatcagcacg ttgttggtct tgaccgggta caggccatag gcgccgttgg acgtcttggg
      301 gccgtcggtc cagcgcacgc gcacgttgcg gatcgtgatg ttgtcgccgc tgttgatctt
      361 gagcgcgtcg cccttggtgt cctcgatggt caggccttcg accgtgaagt cgctcgcctg
      421 cgccagcagg ccctcggggc cggccacctg gttgcggaac gacaggatcg tctgctccat
      481 gccggcgccg cgcacggtca cgccgttgac gcgcagcgac agcccgcggt cgaggtgcca
      541 gcgcccggcc gggatctcga tgaccgagcc gggcctggcg tccagcagct ggccctgcag
      601 cttcttggcg aactcggcgt cgaccggcag gtcgccggcg cgcgcgttcg ggtcggcctg
      661 cttcgtgcag ccgccgagca ggacggccag ccaggccgcg acggcaacag caaggagaac
      721 cgggcgacgg gaagaacggg tcgaatgcat ggcgattcct cgcgaggcgc gacagtacaa
      781 aagatagcaa tggctaccgt tgatgggctc ggtgctttcc ctgtgatgcg aaagcggaac
      841 cggggacacc atagccatgg ctactgtatt gaccttcctc gagccgcctt cacggtggat
      901 acactccccc ccgacacccc acccatcgca tgccgcgcaa accccaggct tccgtcgacg
      961 cccccgccaa cggcgccgcc ggcgtggagc tcgatcgcaa gcgcgacccg acgcagaccc
     1021 gcgcgctcga gacctgcgag cgcatcctcg acgccgccgc caacctgctg ggcgaggtgg
     1081 gcatcgagcg gctgtcgacc aacctgatct gcgagcgcgc cggcctgtcg ccgcccgcgc
     1141 tctaccgcta ctaccccaac aagtacgccg tgctggccga actgggcgtg cggctgatga
     1201 tgagccagaa cgcgctgctg gagagctggg ccacgccggc caccatgaag ctgccggcag
     1261 gcaagttcga ggaccgcatc tgccggctgt tcctcgacac gctggagatg acgcgccggg
     1321 ccaccgccgg cgaatggatc acccgcgcgc tgcgcgccgt cccgtcgctg gcgcccgtgc
     1381 gcctcgagtc gcatgcgcac gtcaccggcc tgctggtggc cgcgttcgcg gtggcctggc
     1441 cggccgtgcc gcagtcgcgt gtccgcctcg tctcgcggct ggccgtggaa gtgatgtact
     1501 cggcccacga gctgatgttc gacgaccccg ccctcgacgc cgaggaagtg ggccgcgtga
     1561 tgtcgcacat ggtcacggcg caggtgatgc agctgcggcg cgagacgcgc tgacgatggc
     1621 cttgcgcgcg cgaggcgtgc gccacgcgcg ccgggagcgc ctcaagcctc cagccggaac
     1681 acgccgacga tctcgaccag ccggccggcc tgcgagttga ggctctccgc ggccgccatc
     1741 gattgctcga cgagcgcggc gttctgctgc gtcatgcggt cgatcaggtt cacggaattg
     1801 gcgaggtcgc ccaggcccga ggtctgccgc tcgatccggt gcgcgatgtt ctcgaggctc
     1861 gcgtgcacgg cctgcacgcc gccgacgatg acggccatgc gctggccgac cacgtgggtc
     1921 agctcgtggc tgccatggat ctcggcgacg ctggccgtga gcagcccgtc gatctccttg
     1981 gccgccgagg cgcagcgctg ggccaggttg cgcacctcgc cggcgaccac gccgaagccg
     2041 cgtccgctct cgcccgcgcg ggccgcctcg accgccgcgt tgagcgccag caggttggtc
     2101 tggaacgcca ggccgtcgat cacgccgacg atctccgaca tccgcgcgct gccggcggac
     2161 aggtggttca tgcgcgccgc gaggtcgtcc atcgccgcct ggccgcggtg cgcggcgtcg
     2221 gcggcgttgg aggcgaggcc gctggcgtcc ttggactccg cggccgattc gcgcagcgcg
     2281 ccgtgcaccg aatccatcgt cgcgttcgcc cgctccaggc gcgcggccgc ctgctcggtg
     2341 cgatggctga ggtcgagatt gcccgccgcc acctgggccg atgcggtcgc cacggcgccg
     2401 gcggccgcgc gcacgtcgcc cagcatgtcg atgaagcgga tgcgcatcgc ctccacggag
     2461 cgcagcatgg cgccgatctc gtcggggccg ccctcgggca cgggctgcga gaggtcgccg
     2521 gcggccacgt ggtccagcgc ggaggtcacc gcggccagcg gccgtccgac ccatcgccgc
     2581 agcagcacgt aaaggccggc gacgagcgcc atcgccgccg cgccgaacag cgccaggaac
     2641 ggcgcgagcg tgcgccactg gccgcgcagg gcctcggcgc cggccgcctc gccgacgacc
     2701 cagtagcccg tcgcctcgct gcgttgcgcg acggccaggc gctggtcgtc gtcgacaccc
     2761 gtggcggcat ggaaccatgg cagcggcttg ccggcctcgg ccttgcgcag cgccgcggcc
     2821 agcgaggcgg g
//
LOCUS       DATCRO010000036         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_22812, whole genome shotgun sequence.
ACCESSION   DATCRO010000036 DATCRO010000000
VERSION     DATCRO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22812"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..328)
                     /gene="grxC"
                     /locus_tag="VIN75_01005"
     CDS             complement(71..328)
                     /gene="grxC"
                     /locus_tag="VIN75_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY8972764.1"
                     /translation="MNPVRMFTTQVCPYCIRAKQLLKQRGVDSIEEIRVDLDPSQRAT
                     MIAETGRRTVPQIFIGDTWVGGCDDLIALDQKGGLVPLLAG"
     gene            complement(455..877)
                     /locus_tag="VIN75_01010"
     CDS             complement(455..877)
                     /locus_tag="VIN75_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8972765.1"
                     /translation="MTFFTDPSVWITIVAAVVSGGLLLWPAITGARSGGVSPAEAVRL
                     MNREKASVIDVSEPGEYAAGHIVGSRNVPMGRLEVAPELPKNKTLPVVLVCASGKRAA
                     AASKALQAKGYAKAVALAGGMTAWKAANLPVEVAATAA"
     gene            1054..1800
                     /gene="gpmA"
                     /locus_tag="VIN75_01015"
     CDS             1054..1800
                     /gene="gpmA"
                     /locus_tag="VIN75_01015"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY8972766.1"
                     /translation="MYKLVLIRHGESTWNLENRFTGWTDVELTATGVAQARQAGELLK
                     EAGYEFDVAYTSVLKRAIWTLWHCLDTMDRTWLPVKQDWRLNERHYGGLQGLNKADMA
                     RQYGDEQVLIWRRSYDTPPPALEADDPRGQRHDLRYAKLSPEQIPLTECLKDTIARVL
                     PCWNEVLAPAIRSGQRIVVAAHGNSIRALVKYLDNIGDADIVGVNIPNGIPLVYELDA
                     DLKPIKSYYLGDAEAAAKAAAAVAAQGKKA"
     gene            1824..2255
                     /locus_tag="VIN75_01020"
     CDS             1824..2255
                     /locus_tag="VIN75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF039954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HEY8972767.1"
                     /translation="MDTLRLRLTYDGAGPAIWTPAPGAFGLQDKDGLLHVGTPDASGR
                     SVFAFALQARPHKSGDVILTGPFAHGTPEVRFLYLGWRRADGAFAQRLKVPLSGITWD
                     AADEAMRRDRPLAATFVDHHPRATKTGANIGGTRSVDWRLD"
     gene            complement(2300..3202)
                     /locus_tag="VIN75_01025"
     CDS             complement(2300..3202)
                     /locus_tag="VIN75_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8972768.1"
                     /translation="MELRHLRYFVAVAEELHFTRAAERLHIAQPPLSQQIRALEDELG
                     VRLFERTRRSVALTDAGRALLGRAHELLAATQALPGELQRIARGEVGQLRIGFSSTLP
                     LTKVLRDVVADWRRTHPDVALHLREMHSALQFEALRAGDLDVGLVRYNERAPDGIRLT
                     LLRRDPLRLVVPAGHRFARRRSVPIADCRDEAFIGFPGDAGTGTGPLLKRLCAQAGFE
                     PRIAQEAREATTQIGLVAAGLGIAVLPAPLEAVRIEGVHYVPLEDEGALLVMSAATRA
                     DEASERVLAFVQRVADLAQVDASA"
     gene            3277..4572
                     /locus_tag="VIN75_01030"
     CDS             3277..4572
                     /locus_tag="VIN75_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8972769.1"
                     /translation="MSATPASLSIPAEGPSRAGAQPLPPPIAPGSAAHRAARWALFVG
                     GFATFGMFYGPQPLLPLFGSAFGLDAAQASEVLSATAGAMALGLIPAGFLAQRYGPKP
                     VMLASIVLGALCSLLCAVAPGYGALVALRALLGLSLAGLPAVSSAWLAEEVDPRALGE
                     SVGLIIAGNAAGGMSSRLVCGLLADHTGWRGAFAGLGVLGVLAAFEFWRSLPPSRRHR
                     RRPLHPRHALADLRAVAGERGLLPLFGLALLLMGSFVSFYNYLGFRLVEAPFALSHAT
                     IGAVFLLYVVGMFSSPWAGRQADRRGSPRVLAAMLAMCALGLLLTLSNALPPIIAGVA
                     LFTFGFFAAHSVASAWVGRLARHSKSLASAAYLTTYYLGASSMGWLGGHAWQAGAWPG
                     VLAFLGVLWLGCVALAWRMARLAPGGVPRTGAREIGRAG"
BASE COUNT          607 a         1699 c         1636 g          676 t
ORIGIN      
        1 aaccgggacg gtgcgggctg catcgcgcat tatgtcgcag gccgaatcac gcgctcgtga
       61 gggatgatcc tcagccggca agcagcggca cgaggccgcc cttctggtcg agcgcgatca
      121 ggtcgtcgca gccgcccacc cacgtatcgc cgatgaagat ctgcggcacc gtgcggcgtc
      181 ccgtctcggc gatcattgtc gcccgctgcg acgggtcgag gtcgacgcgg atctcctcga
      241 tggaatcgac cccgcgctgc ttgagcaact gcttggcgcg gatgcagtag gggcagacct
      301 gggtggtgaa catgcgaacc ggattcatgg gggcgtgctc ctcgctggcg ggccgaggcg
      361 ctggaactgc tgaggtgctc gattttcgcg cggcgccgcc gaccttgcat tccaaggtgg
      421 caaatcgcgg gccgcgccgc gtggcgtcgt tcgctcaggc cgcggtggcc gcgacctcga
      481 cgggcaggtt ggcggccttc caggccgtca tgccgccagc cagcgccacg gccttggcgt
      541 agcccttggc ctgcagcgcc ttggaggcgg cggccgcgcg cttgcccgag gcgcacacca
      601 gcacgaccgg cagcgtcttg ttcttcggca gctccggcgc gacctcgagg cggcccatcg
      661 gcacgttgcg cgagcccacg atgtggcccg ccgcgtactc gcccggctcg ctgacgtcga
      721 tcacgctggc cttctcgcgg ttcatcaggc gcacggcctc ggccggcgag acgccgccgc
      781 tgcgcgcgcc ggtgatggcc ggccacagca gcaggccgcc ggaaacgacg gcggccacga
      841 tggtgatcca gacggagggg tcggtgaaga aggtcaaggc aggcgttcgg aatcgggtgg
      901 aaccggatta taggaacccg cggcgagccg ggccgcgctc cttgcggcgg caaccccttc
      961 ttgccgcatc gcgtcgtccc gcccgatgag cgacgcatag aattcccgct tccccgaccg
     1021 atccaaggcg ccgcacggcg cgaggccccg accatgtaca agctcgtcct gatccgccac
     1081 ggcgaatcca cctggaacct cgagaaccgc ttcaccggct ggaccgacgt cgagctgacc
     1141 gccaccggcg tcgcgcaggc gcgccaggcc ggcgagctgc tgaaggaggc cggctacgag
     1201 ttcgacgtcg cctacacgtc ggtgctcaag cgcgcgatct ggacgctgtg gcactgcctg
     1261 gacacgatgg atcgcacgtg gctgccggtc aagcaggact ggcgcctcaa cgagcgccac
     1321 tacggcggcc tgcagggcct gaacaaggcc gacatggcca ggcagtacgg cgacgagcag
     1381 gtgctgatct ggcgccgcag ctacgacacg ccgccgccgg cgctcgaggc cgacgacccg
     1441 cgcggccagc gccacgacct gcgctacgcc aagctgtcgc ccgagcagat cccgctgacc
     1501 gagtgcctga aggacaccat cgcgcgcgtg ctgccgtgct ggaacgaggt gctcgccccg
     1561 gcgatcaggt cgggccagcg catcgtcgtc gccgcgcacg gcaactcgat ccgcgcgctg
     1621 gtgaagtacc tggacaacat cggcgacgcc gacatcgtgg gcgtcaacat ccccaacggc
     1681 atcccgctcg tctacgagct cgacgccgac ctgaagccga tcaagagcta ctacctgggt
     1741 gacgccgagg ccgccgccaa ggccgccgcg gcggtggcgg cgcagggcaa gaaggcctga
     1801 cctgccgacc ggccgggcgc gcgatggaca cgctgcgcct gcgcctgacc tacgacggcg
     1861 ccggccccgc gatctggacg cccgcgcccg gcgcgttcgg cctgcaggac aaggacggcc
     1921 tgctgcacgt cggcacgccg gacgcgtccg gccgctccgt cttcgcgttc gcgctgcagg
     1981 ccaggccgca caagtcgggc gacgtcatcc tcacgggccc gttcgcgcac ggcacgccgg
     2041 aagtccgctt cctgtacctg ggctggcgcc gcgccgacgg cgcgttcgcg cagcgcctga
     2101 aagtgccgct gtcgggaatc acctgggacg ctgccgacga ggcgatgcgc cgtgaccgcc
     2161 cgctggccgc cacgttcgtc gaccatcacc cgcgggcgac gaagaccggc gccaacatcg
     2221 gcggaacgcg gtccgtcgac tggcgcctgg actgagcttc gcacgtcatg cgcgcggcgg
     2281 tgccgatgcg agcgacccat caggcgctcg cgtcgacctg cgccaggtcg gcgacgcgct
     2341 gcacgaacgc cagcacccgc tccgatgcct cgtctgcccg cgtcgcggcc gacatcacca
     2401 gcagcgcgcc ctcgtcttcc agcggcacgt agtgcacgcc ctcgatgcgc acggcctcca
     2461 gcggcgccgg cagcaccgct atgccgaggc ctgcggcgac caggccgatc tgcgtggtgg
     2521 cctcgcgcgc ctcctgggcg atgcgcggct cgaagcccgc ctgcgcgcac aggcgcttga
     2581 gcagcgggcc cgtgccggtg ccggcgtcgc ccgggaagcc gatgaacgcc tcgtcgcggc
     2641 agtcggcgat gggcaccgaa cgacggcgcg cgaaccggtg gcccgccgga accaccaggc
     2701 gcaacgggtc gcggcgcagc agcgtcaggc ggatgccatc gggcgcgcgc tcgttgtagc
     2761 gcaccagtcc gacgtcgagg tcgcccgcgc gcagcgcctc gaattgcagc gcggagtgca
     2821 tctcgcgcag gtgcagcgcg acgtcgggat gcgtgcggcg ccagtccgcg acgacgtcgc
     2881 gcagcacctt ggtcagtggc agcgtcgacg agaacccgat gcgcagttgc ccgacctcgc
     2941 cgcgcgcgat gcgctgcagc tcgccaggca gcgcctgcgt cgcggcgagc agttcgtgcg
     3001 cgcgcccgag cagcgcccgg cccgcgtcgg tcaacgccac cgagcggcgg gtgcgctcga
     3061 acagccgcac gcccagttcg tcctccagcg cgcggatctg ctggctcagc ggcggctggg
     3121 cgatgtgcag gcgttcggcg gctcgcgtga agtgcagctc ttcggccacg gcgacgaagt
     3181 agcgcaggtg tcgaagctcc attgagtctt ccaacgtctc aatatatcga caatcatata
     3241 ttggactgac taagggtgtt tccctaggat gcgctcatgt ccgccacgcc cgccagcctg
     3301 tcgatccccg ccgagggccc gtcgcgggcc ggcgcccaac cgctgccccc gccgatcgcg
     3361 ccgggcagcg ccgcccaccg cgccgcgcgc tgggccctgt tcgtcggcgg cttcgccacc
     3421 ttcggcatgt tctatgggcc gcagcctctg ctgccgctgt tcggcagcgc gttcgggctg
     3481 gacgcggcgc aggccagcga agtgctttcg gccacggccg gcgccatggc gctgggcctg
     3541 atccccgccg gcttcctcgc ccagcgctac ggccccaagc cggtgatgct cgcgtccatc
     3601 gtgctgggcg cgctgtgcag cctgctgtgc gcggtggcac ccggctacgg cgcgctggtc
     3661 gcgctgcgcg cgctgctggg gctgagcctg gccgggctgc cggcagtctc gtcggcatgg
     3721 ctggccgaag aggtcgaccc gcgtgcgctc ggggagtcgg tcggactgat catcgccggc
     3781 aacgcggcgg gtggcatgtc cagccgcctc gtctgcggcc tgctggccga ccacaccggc
     3841 tggcgcggcg cgttcgcggg cctgggcgtc ctcggcgtgc tcgcggcgtt cgagttctgg
     3901 cgcagcctgc cgccgtcgcg ccgtcatcgc cggcgcccgc tgcatccgcg tcatgcgctg
     3961 gccgacctgc gtgccgtcgc gggcgagcgc ggcctcctgc cgctgttcgg cctggcgctg
     4021 ctgctgatgg gcagcttcgt gagcttctac aactacctgg gtttccggct cgtcgaggcc
     4081 ccgttcgcgc tgtcgcatgc caccatcggc gcggtgttcc tgctgtacgt cgtgggcatg
     4141 ttctcgtcgc cctgggccgg caggcaggcc gaccgccgcg ggtcgccgcg cgtgctggcc
     4201 gcgatgctgg cgatgtgcgc gctcggcctg ctgctgacgc tgtcgaacgc gctgccgccg
     4261 atcatcgccg gcgtcgcgct gttcacgttc ggcttcttcg cggcgcactc ggtggccagc
     4321 gcctgggtcg gccggctcgc gaggcactcg aagtcgctcg cctccgcggc ctacctcacc
     4381 acgtactacc tcggcgccag cagcatgggc tggctcggcg gccatgcctg gcaggccggc
     4441 gcgtggcccg gcgtgctcgc gttcctcggc gtgctgtggc tgggctgcgt ggcgctcgcg
     4501 tggcgcatgg cgcgcctcgc gcccggcggc gtgccgcgca cgggagcgcg ggagatcggc
     4561 cgggcgggtt agccgctggc gccacccgcg ccagccacat gttgacgaac ccgcatcc
//
LOCUS       DATCRO010000037         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_225298, whole genome shotgun sequence.
ACCESSION   DATCRO010000037 DATCRO010000000
VERSION     DATCRO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225298"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VIN75_01035"
     CDS             <1..296
                     /locus_tag="VIN75_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972770.1"
                     /translation="RQAKVAELRKLDLKADPVNSPVALAPGLSLGQFTSQADATRRLD
                     ELTRRGVHTARVAVLDAPIVLRHLQVHATDTAWRRAAVGQRFDSCPADTASNT"
     gene            complement(556..2610)
                     /locus_tag="VIN75_01040"
     CDS             complement(556..2610)
                     /locus_tag="VIN75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8972771.1"
                     /translation="MARLSRLLGAVSLGIVVTTAVGASVVAVESNPVRKAPSQLAEPA
                     GNGRVIVKFKERSSILSATASSTGAATGPQKAAVLGSRLGLALTDGRILGPRTQVLMT
                     TGMNSTALAAAVASDPDVEWAEVDHRRFIQSAPVNDPLYPDNLSAAAAPNGPVAGQWY
                     LRAPTSTVLASIDVEPAWIITKGSTAVVIGDIDTGITSHPDLNSKILPGYDFIADVPT
                     ANDGNGRDSDPSDPGDWVTATENSTKNGAFFGCNDNGTGAAVAENSSWHGTQTAGILG
                     AATNNSTGMAGTAYNVPVIPARALGKCGGYDSDIIAAANWLGGIAVSGVPTNTHPVRV
                     INMSLGSQGACTNGYQDVLTALRNKGVVVVAAAGNDEGLAVGTPANCQPASTDSDKTP
                     IVIAVAGLRHAGTKVGFSDIGPEVTIAAPGGNCVNSTGACLYPILTTSNTGLTSPGTA
                     TYSDGMSHPSLGTSFATPLVAGTVALMLSANPSLSNADVVRILKSTARTFPTTSDTGT
                     VPVCTTPVSGGAAQDECICTTSTCGAGMLDAGAAVNKVWTEAGGAVIAPVAAISASSA
                     TVQASSSITLDGTGSTTSSTGAAITSYAWSIDNTAVATLSATSGPSVTLTGVAAGTAT
                     VTLTITDADNRTATTTHAITVTAASSGGGGGGGGGGGAANPAWLLALAVAGLLLRPRK
                     RA"
BASE COUNT          346 a          936 c         1001 g          423 t
ORIGIN      
        1 cccgccaggc caaggtcgcc gaactgcgca agctcgacct gaaggccgac ccggtgaact
       61 cgccggtggc gctcgcgccg gggctgtcgc tgggccagtt caccagccag gccgacgcga
      121 cccggaggct cgacgagctg accaggcggg gcgtgcacac cgcgcgcgtc gcggtgctcg
      181 acgcccccat cgtcctgcgc cacctgcagg tgcacgcgac cgacaccgcg tggcgccgcg
      241 cggccgtggg tcagcgcttc gacagctgtc cggcggacac ggcgtccaat acctgacctg
      301 cacacgtagg gcaggtcagg cgcagccgta acccgccgtt cggttgcgcg ccgatccgcc
      361 gacgctatcg atacgaaggc ccctttccaa ctcgcaggcg aacggcgggt tacgcctgcg
      421 gctaacccgc cctgcgagga catcggcgct ccggcgacgc cggttccttc cgtgcaccac
      481 gcatgaaaaa aagccgctgc atcgctgcag cggccttcgt cgttcatgca cgcgcgccgg
      541 cttggcgcgc gggcgtcacg cgcgcttgcg cgggcgcagg agcaggccgg cgacagccag
      601 ggccagcagc caggccgggt tcgcggcgcc gccgccgccg ccgccgccgc cgccaccgcc
      661 cgaggaggcc gccgtcacgg tgatcgcgtg ggtggtggtc gcggtgcggt tgtccgcgtc
      721 ggtgatcgtg agcgtgacgg tggccgtccc cgccgcgacg ccggtcagcg tcacgctggg
      781 gccggaggtg gcgctgagcg tcgcgaccgc cgtgttgtcg atcgaccagg cgtagctggt
      841 gatcgccgcg ccggtgctgc tggtggtgct gccggtgccg tcgagcgtga tcgagctgga
      901 cgcctgcacc gttgcggacg aggcggagat cgccgccacc ggcgcgatga ccgcgccgcc
      961 ggcttccgtc cacaccttgt tgaccgccgc gccggcatcg agcatgcccg cgccgcaggt
     1021 gctggtggtg cagatgcact cgtcctgcgc cgcgccgccg gacacgggcg tcgtgcagac
     1081 cggcacggtg cccgtgtcgc tggtggtggg gaacgtgcgc gcggtcgact tcaggatgcg
     1141 cacgacgtcc gcgttgctca gcgacgggtt ggccgacagc atcagcgcga cggtgccggc
     1201 cacgagcggc gtcgcgaagc tggtgcccag gctcgggtgg ctcatgccgt ccgagtaggt
     1261 cgcagtgccc ggcgacgtga gccccgtgtt gctcgtggtc aggatcgggt acaggcaggc
     1321 gcccgtcgaa ttgacgcagt tgccgcccgg cgcggcgatc gtgacctcgg ggccgatgtc
     1381 ggagaagccg accttggtgc ccgcgtggcg caggccggcg acggcgatca cgatcggcgt
     1441 cttgtcggag tcggtcgatg ccggttggca gttggcgggc gtgcccacgg ccaggccttc
     1501 gtcgttgccg gcggcggcga cgaccaccac gcccttgttg cgcagcgcgg tcagcacgtc
     1561 ctggtagccg ttcgtgcagg cgccctgcga gcccaggctc atgttgatga cgcgcacggg
     1621 gtgcgtattc gtgggcacgc cgctgaccgc gatgccaccg agccaattgg cggccgcgat
     1681 gatgtcggag tcgtagccgc cgcacttgcc gagcgcgcgc gccgggatca ccggcacgtt
     1741 gtacgccgtg ccggccatgc cggtcgaatt gttggtggcc gcgcccagga tgccggccgt
     1801 ctgcgtgccg tgccagctgc tgttctcggc cacggcggcg ccggtgccgt tgtcgttgca
     1861 gccgaagaac gcgccgttct tggtcgagtt ctcggtcgcg gtcacccagt cgcccgggtc
     1921 ggacggatcg gaatcgcggc cgttgccgtc gttggcggtg ggcacgtcgg cgatgaagtc
     1981 gtagcccggc aggatcttgc tgttcaggtc ggggtgcgac gtgatgccgg tgtcgatgtc
     2041 gccgatgacg acggccgtcg agcccttggt gatgatccag gccggttcga cgtcgatgct
     2101 ggccagcacc gtcgatgtcg gcgcgcgcag gtaccactgg ccggccaccg ggccgttcgg
     2161 cgccgccgcg gcgctcaggt tgtccggata cagcgggtcg ttgaccggcg cggactggat
     2221 gaagcggcga tggtcgacct cggcccactc gacatcggga tcgctggcga cggccgcagc
     2281 cagcgccgtc gagttcatgc ccgtggtcat gagcacctgc gtgcgcgggc ccaggatgcg
     2341 gccgtcggtc agggccaggc ccagtcgcga gccgagcacg gccgccttct gcgggcccgt
     2401 ggcggcgccg gtggacgagg cggtggccga caggatcgac gagcgctcct tgaacttgac
     2461 gatgacgcgc ccgttgccgg ccggttcggc cagctggctc ggcgccttgc gcaccggatt
     2521 gctctcgacg gcgacgaccg acgcccccac ggccgtcgtc accacgatcc ccaggctgac
     2581 tgcgcccagc agtcgactca agcgtgccat cgcactctcc gaaaggttgg ttccgccccg
     2641 ccacgcgagg ccttgttatc gatcagagtc tagtgggatc cgggttgcag actcgttgcg
     2701 cgggtg
//
LOCUS       DATCRO010000038         6183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1012829, whole genome shotgun sequence.
ACCESSION   DATCRO010000038 DATCRO010000000
VERSION     DATCRO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012829"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VIN75_01045"
     CDS             complement(<1..941)
                     /locus_tag="VIN75_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY8972772.1"
                     /translation="MVTTHNLGFPRIGARRELKFALEAYWKGESSRDALVSIGAQLRQ
                     RHWIHQANLDLAPVGDFAFYDQMLDMSFTLGNLPERVRGFHGDALDNYFRVARGRSAH
                     TADHAQCCGGGVAAGEMTKWFDTNYHYIVPEFTADTTFTLDASRLLDQLAEARAQNVK
                     AKPVIIGPVTYLALGKAKDGSNRLDLLPRLVPVYCELLDALARAGVEWVQIDEPILVT
                     ELDADWQHALRTAYHDLKSARVKLLLATYFGQLMENQHLAANLPVAGLHVDAVRGRDD
                     VIPLLGLLSPVKVLSLGVIDGRNIWKTDLNAVLDWLEP"
     gene            1052..1963
                     /locus_tag="VIN75_01050"
     CDS             1052..1963
                     /locus_tag="VIN75_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972773.1"
                     /translation="MLERIHLAIVRQVDRLGSMTAAAESLHLTQSAVSHSMRKLEEQL
                     GADIWLREGRSLRLTQAGQYLLQVANRVLPQLELAETRIAQYAEGERGTLRIGMECHP
                     CYQWLLKIVSPYLARWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLHKPGLRFVP
                     VFDYEQVLVVHQGHQLAGVAYVRPEQLSDQVLITYPVPVDRLDVYTQFLTPAGITPRR
                     QKAIETTDIMLQMVASGRGVAALPRWLAEEYAARMPVVPVRLGRHGIPKQIFLGAREA
                     DVDVDYLKAFVDYARRPARTAARRRAA"
     gene            1960..2586
                     /locus_tag="VIN75_01055"
     CDS             1960..2586
                     /locus_tag="VIN75_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEY8972774.1"
                     /translation="MTSGPTPRAWIRLPSGAALDLINPSPHAWTDEDLALRLARTYRW
                     GGESTWTWPLSVAQHSLLVLALRREWSDTPLTLAGQRAELLHDAEEGFLGFDCISPLK
                     RVLGQPFADVADRLMHAVSQRYALPDWTPASHREHKHADSVAAASEAVHCTGWSRDEV
                     RDVLGIAYPVLDVDPLQAVYGGTPWEPWPSDVAAERFLDELRRLARDV"
     gene            complement(2835..3695)
                     /locus_tag="VIN75_01060"
     CDS             complement(2835..3695)
                     /locus_tag="VIN75_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY8972775.1"
                     /translation="MSTRKTLKTLVNARRGAIVPGAFNALSARVIEDLGYEAIYVTGA
                     GVTNMNLGLPDQGFMGLAEIAEATSRIRDAVGLPLLVDMDTGFGNALNTYHSVRVLER
                     AGADCVQLEDQVAPKRCGHFSGKEVISAEEAVSKIKAAVDARTDPDFLIMARTDAAAT
                     HGFEAAVERAQRFAEAGADILFVEAVTTADEIRALPRRLGPPQLMNMVIGGKTPIFGA
                     EELGRLGYGIVLYANAALQGALAGMQRALTVLRDTKRVDEDPDLVAPFKERQRLVGKP
                     FWDELEKKYT"
     gene            complement(3712..4905)
                     /gene="prpF"
                     /locus_tag="VIN75_01065"
     CDS             complement(3712..4905)
                     /gene="prpF"
                     /locus_tag="VIN75_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEY8972776.1"
                     /translation="MSHVPQIKIPATYMRGGTSKGVFFRLQDLPLSAQQPGAARDKLL
                     MRVIGSPDPYGKQIDGMGGATSSTSKTVIVSRSTRPDHDVDYLFGQVAIDQAFVDWSG
                     NCGNLSAAVGPFAVAGGLIDAARIPQNGTVVVRIWQANIGKTIVAHVPVTHGQVQETG
                     DFELDGVTFPAAEVALEFMDPAADEGEGGGSMFPTGHVVDELDVPGIGKLQATLINAG
                     IPTIFVDAQAIGYTGTELQGAINEDAKALAMFETLRAHGALRMGLIQQLVEARTRQHT
                     PKIAFVARPADYVASSGRRVAARDIDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGT
                     LVNLAAGGGEARTAVRFGHPSGTLRVGAEARLVDGQWQVAKALMSRSARVLMEGNVRV
                     PGDSF"
     gene            complement(4914..>6183)
                     /locus_tag="VIN75_01070"
     CDS             complement(4914..>6183)
                     /locus_tag="VIN75_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8972777.1"
                     /translation="SKAVQLYLEEAGLLPELEKLGFGIVAFACTTCNGMSGALDPQIQ
                     QEIIERDLYATAVLSGNRNFDGRIHPYARQAFLASPPLVIAYAIAGTIRFDIEKDALG
                     TDANGQPVTLKDIWPTDAEIDAIVASSVKPEHFRKVYDPMFKLQKDTGEKISPLYAWR
                     PQSTYIRRPPYWEGALAGERTLRGLRPLAVLPDNITTDHLSPSNAILPDSAAGEYLAK
                     MGLPEEDFNSYATHRGDHLTAQRATFANPQLVNEMAVVDGKVKKGSLTRVEPDGNVVR
                     MWEGIETYMARKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLV
                     GMGVLPLEFKPGTNRLTLAIDGTEVFDVVGERKPRADLTLVIHRKDGERVEVAVTCRL
                     DTAEEVSIYEAGGVLQRFAQDFLASSAEAA"
BASE COUNT          881 a         2134 c         2158 g         1010 t
ORIGIN      
        1 ggctcgagcc agtccagcac cgcgttcagg tcggtcttcc agatgttgcg gccgtcgatc
       61 acgcccagcg acagcacctt gaccggcgac agcaggccca gcagcggaat cacgtcgtcg
      121 cggccgcgga cggcatcgac gtgcagcccg gccaccggca ggttggcggc caggtgctgg
      181 ttctccatca actggccgaa gtacgtggcc agcagcagct tgacccgggc gctcttgagg
      241 tcgtggtatg ccgtgcgcag cgcgtgctgc cagtcggcgt cgagctcggt cacgaggatg
      301 ggctcgtcga tctgcaccca ctcgacgccc gcccgcgcca gcgcgtccag cagctcgcag
      361 tagaccggca ccaggcgcgg cagcaggtcg agccggttcg agccatcctt ggccttaccc
      421 agcgccaggt aggtgaccgg gccgatgatc accggcttgg ccttgacgtt ctgcgcgcgg
      481 gcctcggcca gctggtcgag caggcgcgag gcgtcgagcg tgaacgtggt gtcggcggtg
      541 aactcgggga cgatgtagtg gtagttggtg tcgaaccact tggtcatctc gcccgcggcc
      601 acgccgccgc cgcagcactg cgcgtggtcg gcggtgtgcg cggagcggcc gcgcgcgacc
      661 cggaagtagt tgtccagcgc gtcgccgtgg aagccgcgca cgcgctcggg caggttgccg
      721 agcgtgaagc tcatgtccag catctggtcg tagaacgcga agtcgccgac gggcgccagg
      781 tcgagattgg cctggtggat ccagtggcgc tggcgcagct gcgcgccgat ggagacgagc
      841 gcgtcgcgcg aggactcgcc cttccagtac gcctccagcg cgaacttcaa ttcgcgcctg
      901 gcgccgatgc gcgggaagcc caggttgtgc gtggtgacca tgtcgaactc ttcttcgtct
      961 tgaactggga agccgccatc ctaggaagat cggctcatga cgtaaaatgg tcttatttca
     1021 ggaatcgatc gattccactc atggattgtc cgtgctcgaa cgcatccacc tcgcgatcgt
     1081 ccggcaggtc gacaggctcg gctcgatgac ggccgcggcc gagtcgctgc acctgacgca
     1141 gtcggccgtc agccactcga tgcgcaagct cgaggagcag ctgggcgccg acatctggct
     1201 gcgcgagggc cgcagcctgc gcctcacgca ggccggtcag tacctgctgc aggtggccaa
     1261 tcgcgtgctg ccgcagctcg agctggccga gacgcgcatc gcgcagtacg ccgagggcga
     1321 gcgcggcacg ctgcgcatcg gcatggaatg ccatccgtgc taccagtggc tgctcaagat
     1381 cgtctcgccc tacctcgcgc gctggcccga cgtcgacgtc gacgtgaagc agaagttcca
     1441 gttcggcggc atcggcgcgc tgttcggcta cgagatcgac ctgctggtca cgcccgaccc
     1501 gctgcacaag cccggcctgc gcttcgtgcc ggtgttcgac tacgagcagg tgctcgtcgt
     1561 gcaccagggc caccagctgg cgggcgtcgc gtacgtcagg cccgagcagc tgtccgacca
     1621 ggtgctgatc acctaccccg tgcccgtcga ccgcctcgac gtctacacgc agttcctgac
     1681 gcccgcgggc atcacgccgc ggcgccagaa ggcgatcgag accaccgaca tcatgctgca
     1741 gatggtggcc agcggccgcg gcgtcgccgc gctgccgcgc tggctggccg aggagtacgc
     1801 cgcgcgcatg cccgtcgtgc cggtgcgcct gggccgccac ggcatcccca agcagatctt
     1861 cctgggcgcg cgcgaggccg atgtcgacgt cgactacctc aaggccttcg tcgactacgc
     1921 gcgccggccg gcgcgcaccg ccgcaagaag gcgcgcggca tgacgagcgg tccgactccc
     1981 cgcgcctgga tccggctgcc ctcgggcgcg gcgctcgatc tcatcaaccc gtcgccccac
     2041 gcgtggacgg acgaggatct cgcgctgcgc ctcgcgcgca cctaccgctg gggcggcgag
     2101 tcgacgtgga cgtggccgct gtcggtggcg cagcactcgc tgctggtgct ggcgctgcgg
     2161 cgcgaatggt cggacacgcc gctgacgctc gccgggcagc gcgccgaact gctgcacgac
     2221 gccgaggaag gcttcctcgg cttcgactgc atctcgccgc tcaagcgcgt gctgggccag
     2281 cccttcgccg acgtcgccga ccggctgatg cacgcggtgt cgcagcgcta cgccctgccg
     2341 gactggacgc ccgcgtcgca ccgcgagcac aagcatgccg actcggtggc cgcggcctcc
     2401 gaggccgtgc actgcaccgg ctggtcgcgc gacgaggtgc gcgacgtgct ggggatcgcg
     2461 tatccggtgc tggatgtgga tccgctgcag gcggtctacg gcggcacgcc gtgggagccg
     2521 tggccgtcgg atgtggcggc cgagcgcttt ctcgacgagt tgaggcgcct cgctcgcgac
     2581 gtctgaacgg cgccggaggg gtctggcctc tccttcatcc gcggagaagc gtcgatcgca
     2641 gtgactcgcc gcatcgcgga tgaaggcaaa ggcctgaccc cgatcacccg ggcagcggtc
     2701 gatcgaggtc aggccttcgc ctcggcatcg cactcgatgc ttccgctcac gaccgcaatg
     2761 ccgcgcgatg ccgaggagag gccggacccc tccggcttcg cggcgccagt catgccatgc
     2821 cgcaaccggg aacctcaggt gtacttcttc tccagctcgt cccagaacgg cttgcccacg
     2881 aggcgctgcc gctccttgaa cggggcgacg aggtccggat cctcgtcgac ccgcttcgtg
     2941 tcgcgcagca ccgtcaacgc gcgctgcatg ccggcgagcg cgccctgcag cgcggcgttg
     3001 gcgtacagca cgatgccgta gcccagccgg cccagctcct cggcaccgaa gatgggcgtc
     3061 ttgccgccga tgaccatgtt catcagctgc ggcggtccca ggcgcctggg cagcgcgcgg
     3121 atctcgtcgg cggtggtcac ggcctcgacg aacaggatgt ccgcgccggc ttccgcgaag
     3181 cgctgcgcgc gctcgacggc ggcctcgaag ccatgggtgg cggcggcgtc ggtgcgggcc
     3241 atgatcagga aatcgggatc ggtacgcgcg tcgacggccg ccttgatctt gctgaccgcc
     3301 tcctcggcgg agatcacttc cttgcccgag aaatggccgc agcgcttggg cgcgacctgg
     3361 tcttccagct gcacgcagtc ggcgccggcg cgttccagca cgcgcaccga gtggtacgtg
     3421 ttcaacgcgt tgccgaagcc ggtgtccatg tcgaccagca gcggcaggcc gaccgcgtcg
     3481 cggatgcggc tggtggcctc ggcgatctct gccaggccca tgaagccctg gtccggcagg
     3541 cccaggttca tgttggtgac gccggcgccg gtgacataga tcgcctcgta gccgaggtcc
     3601 tcgatcacgc gcgccgacag cgcgttgaac gcgcccggca cgatcgcgcc gcggcgggcg
     3661 ttcacgaggg tcttgagggt cttgcgagtg gacatgtgcg ggatctcgaa atcagaaact
     3721 gtcgcccggg acgcggacgt tgccttccat cagcacgcgc gcgctgcggc tcatcaaggc
     3781 cttggcgacc tgccactggc catcgaccag ccgcgcctcg gcgccgacgc gcagcgtgcc
     3841 cgacgggtgc ccgaagcgca ccgcggtgcg cgcctcgccg ccgccggccg cgaggttgac
     3901 cagcgtgccg gggatggccg ccgcggtgcc gatcgccacc gccgccgtgc ccatcatcgc
     3961 gtggtgcagc ttgcccatcg acatcgcgcg caccagcagg tcgatgtccc tggctgcgac
     4021 gcgcctgccg ctggatgcga catagtcggc gggcctggcc acgaacgcga tcttgggcgt
     4081 gtgctggcgc gtgcgcgcct cgaccagctg ctggatcagg cccatgcgca gcgcaccgtg
     4141 cgcgcgcagc gtctcgaaca tcgccagcgc cttggcatcc tcgttgatcg cgccctgcag
     4201 ctcggtgccc gtgtagccga tggcctgcgc gtcgacgaag atcgtgggga tgccggcgtt
     4261 gatcagcgtg gcctgcagct tgccgatgcc cggcacgtcc agctcgtcga cgacatggcc
     4321 ggtggggaac atcgagccgc cgccctcgcc ttcgtcggcc gcgggatcca tgaactcgag
     4381 cgcgacctcc gcggccggga acgtgacgcc gtccagctcg aagtcgcccg tctcctgcac
     4441 ttgcccgtgc gtgacgggca cgtgcgcgac gatggtcttg ccgatgttgg cctgccagat
     4501 gcgcacgacc accgtgccat tctgcgggat gcgcgcggca tcgatcaggc cgccggcgac
     4561 cgcgaacggg ccgactgccg ccgacaggtt gccgcagttg ccgctccagt cgacgaaggc
     4621 ctggtcgatc gcgacctggc cgaacaggta gtcgacgtcg tggtcggggc gcgtgctgcg
     4681 cgagacgatc accgtcttgc tggtggacga cgtcgcgccg cccatgccgt cgatctgctt
     4741 gccgtacggg tcgggactgc cgatgacgcg catcagcagc ttgtcgcgcg cggcgccggg
     4801 ctgctgggcg ctcagcggca ggtcttgcaa gcggaagaac acacccttgc tcgttccgcc
     4861 acgcatgtag gtggcgggga tcttgatctg gggaacgtgg gacatggtgt ctttcaagct
     4921 gcctcggccg acgaggccag gaaatcctgc gcgaagcgct gcagcacgcc gccggcctcg
     4981 tagatcgaca cttcctcggc ggtgtcgagt cggcaggtga cggccacctc gacgcgctcg
     5041 ccgtccttgc gatggatgac cagcgtcaga tcggcgcgcg gcttgcgctc gccgacgacg
     5101 tcgaacacct ccgtgccgtc gatggccagc gtcaggcggt tcgtgcccgg cttgaactcc
     5161 agcggcagca cgcccatgcc cacgaggttg gtgcggtgga tgcgctcgaa gccttcggcg
     5221 acgatggcct cgacgcccgc cagccgcacg cccttggccg cccaatcgcg cgacgagccc
     5281 tggccgtagt cggcgccggc gatgatgatc agcggctgct tgcgcgccat gtaggtctcg
     5341 atgccttccc acatgcgcac gacgttgccg tcgggttcca cccgcgtgag cgagcccttc
     5401 ttcaccttgc cgtcgacgac cgccatctcg ttgaccagct gcgggttcgc gaacgtggcg
     5461 cgctgcgcgg tgaggtggtc gccgcggtgc gtcgcgtagg agttgaagtc ctcctcgggc
     5521 aagcccatct tggccaggta ctcgccggcc gcgctgtcgg gcaggatcgc gttggacggc
     5581 gacaggtggt cggtggtgat gttgtccggc agcaccgcca gcgggcgcag gccgcgcagc
     5641 gtgcgctcgc cggccagcgc gccctcccag tacggcgggc ggcggatgta ggtgctctgc
     5701 ggacgccacg cgtacagcgg gctgatcttc tcgcccgtgt ccttctgcag cttgaacatc
     5761 gggtcgtaga ccttgcggaa gtgttccggc ttgacgctgc tcgcgacgat cgcgtcgatc
     5821 tcggcgtcgg tcggccagat gtccttcagg gtcaccggct ggccgttggc gtcggtgccc
     5881 agcgcatcct tctcgatgtc gaagcggatg gtgccggcga tggcgtaggc gatgaccagc
     5941 ggcggcgacg cgagaaaggc ctgcctggcg tagggatgga tgcggccgtc gaagttgcgg
     6001 ttgcccgaga gcacggcggt cgcgtacagg tcgcgctcga tgatctcctg ctggatctgc
     6061 ggatccagcg caccgctcat gccgttgcag gtcgtgcagg cgaacgcgac gatgccgaag
     6121 ccgagttttt cgagctcggg cagcaggccg gcttcttcga gatacagctg cacggccttg
     6181 gat
//
LOCUS       DATCRO010000039         9507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_967938, whole genome shotgun sequence.
ACCESSION   DATCRO010000039 DATCRO010000000
VERSION     DATCRO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967938"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VIN75_01075"
     CDS             <1..924
                     /locus_tag="VIN75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF036801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8 and S53 subtilisin kexin sedolisin"
                     /protein_id="HEY8972778.1"
                     /translation="VYTAAASVTGMTTTVTPSTLSLAPGQSASFTVTMAPAASTPKFT
                     WQYGSLTWNDGAGHNVRSPIQVALGQSISAPSDLSATTVSGSRMFTISTGFAGHASAN
                     KGMRDVTMGPATTLTPNSNVDAAAVCKATGPTANVAVYNFTVPAGAIVARFALRQADV
                     SGATDDNDVAVVLPSGAVAASEGGTSHETVELLNPAAGNYKVCVASYAAASGSSTHQL
                     SSWIVSPGDTAGGAFNVAMPSTVYSGGTSTVGAVWSGLSAGHRYIGGIQYLDASSAVA
                     AATALSIDTTPGTPLDMPTPIANDKASVTKK"
     gene            complement(1015..1470)
                     /locus_tag="VIN75_01080"
     CDS             complement(1015..1470)
                     /locus_tag="VIN75_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972779.1"
                     /translation="MNFIIRRPVRRATLVVASLLAIAGCASAVDPAAPAAAAAAPPKS
                     VALHARVVELIGDAACDGQAQCHAVGLGAKPCGGAETWLPWSSKITDAHALQDAVQAL
                     AQARVEENKASGLASDCMRRPDPSVVCRPRASDGRKSCQLGQGGTDSAI"
     gene            complement(1533..2828)
                     /locus_tag="VIN75_01085"
     CDS             complement(1533..2828)
                     /locus_tag="VIN75_01085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8972780.1"
                     /translation="MFRSSPDYVDSYYAATSRHLQTSRPSLQENLDVDVCVVGAGFFG
                     LYSAMELAKAGKRVAIVEASRVGWGATGRNGGQMIIGFPCGMQRLKGVMGHERAKRMF
                     DASREAVTDIRGIMAQHQIDCDLVQGHLEVAVLKRRVADLTDWIEECVNEWGYDKLQL
                     VEKKDLPKYLNSARFQAGIIDPEGCHIHPLKYVLGLARAVEGLGVRIFEQSKVDGYVE
                     NQGDRIQVKVGPHVVRCDQLVLATNAYVDKLDPKLAARTLPVGTFIGVTAPLGEAVAR
                     SLIPHNHAVYDNQFILEYFRMTADHRLLFGGKCTYLGGTPSNLKDAMRVNIARAFPQL
                     ANVQMDYAWGGHIDITMRRMPDWGQRDGRVFWAQGFCGHGLVPTRVAAKVVTDKMLAR
                     SDELDWFAPIVNPPFPGGEAIGGLMQAVGMSYYRVRDFV"
     gene            complement(2873..4393)
                     /locus_tag="VIN75_01090"
     CDS             complement(2873..4393)
                     /locus_tag="VIN75_01090"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8972781.1"
                     /translation="MTASSDFHSATALAAPHRAVVQAALDSLSAELGVPVTAIECAVG
                     DFTSVARGKSVGHQDFVGMAGCRFPSVVFGLTLTAGEPEAVFGPLLPASYLDISLVPD
                     LATLRAQPGRAGTAAVICEPAGPLPAGRYDRAVCASEYSPRAALRRVLARLEQAGLAA
                     TVAPELELFLVDRREEGGQVSLHAPASRAGAPARERACEAYSLERTGHFADYFDALFA
                     ACQLQGIPVCGHAHEAAYSQYEVNFMPGEPLAQADAVFRFKRLARQVAAQFGFLCTFA
                     PKPFLDQPGTGMHWHFSIHDSPARSAPRPQGAHAGSLRSGRALAGEGTDVPGAHTNAF
                     AHPDGRDTPVLGAFIGGLQRHADAAMAFFAPHDMAFDRIRLSNASPSSASWGHDDRAV
                     AFRIPQASAQNRRIENRLPGGDANPYLVVAATLGMGLAGIEQGLRPAGESAGVALPRS
                     LPEACDALEASALMRELFGAPLVDLFVALKRHESAERHAAADPRHHWDLVHLVELA"
     gene            4483..6027
                     /locus_tag="VIN75_01095"
     CDS             4483..6027
                     /locus_tag="VIN75_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8972782.1"
                     /translation="MASNAPTHLAGDALVDWLQHAWAHPAADEAALPIGARLTQQMRR
                     AILERVVPPAHRLPSTRTLAQALRIARNTVVPVYEQLRAEGFVVAGQGSGTYVCRIAP
                     EALPAAAKATEPAEAPRAASPLAFSRRGRRYHSHPLHEFWTRQPFCPGQFDFELFPHR
                     LWNRLQQRQLRRADPIQLEQGEPGGAPELRQAIAEHVRATRGVRCAPDQVIITDGTGE
                     SIDLVARLMCDPGDTVLLEDPGYWAATQVLSDHGLRLRPMPVDAHGLPVPMLRAGERA
                     PRVVYLTPSNQFPTGGAMPLARRLEWLRFAARHGTLLLEDDYDSEYRYEGAPFPSLQG
                     QDAAGRVIYLGTFSKTVYPGIRVAFLVVPQGLQRVFADASADFYRDGDQIVQQVLADF
                     MREGHYAAHVRTLRREYALRREAMLRALAEALPREFATQRLSVVSGARGVHLTLALPD
                     DVDDRAVARAAIAHGVTAIPLSVYAMHVGWRGLVLSFAATPVPRIAPLVGTLAPVLRN
                     ALAHRP"
     gene            6192..7406
                     /locus_tag="VIN75_01100"
     CDS             6192..7406
                     /locus_tag="VIN75_01100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8972783.1"
                     /translation="MTIDGRSLLAVVAALCIVFSIVVLALERRIRQTMPGLRLWAIAN
                     LFLGAAAAAHMLQGHVPVWVPAILGNWTMLVGRSTSVAALRQFDRQPVPIAWLVGATA
                     LFMLGIAGTLVWPEPRVRAMIMVGGMAVLALWATYSLAVGASPSVSRVLTMVGMGGWG
                     LANLWRLWTTAHLPEGAQVLNLSDPTVAAYLCALVIMDVFCNIGFVLLITDRMHEWVL
                     QLARTDHLTGALSRGALYTQATRDLQLARRQRTYTAAFIVDIDRFKTINDSHGHAAGD
                     AILRHVAQHGQAVLRCTDLFCRYGGDEFVAILPETDLPTAAAIAERLRVAVGEPVPGA
                     APLASGLCGVTVSIGVAAVAPGCEAIDELIAQADEALYDAKHAGRNRTRVADEEPATP
                     SKPPLRVIQGRN"
     gene            complement(7469..>9507)
                     /locus_tag="VIN75_01105"
     CDS             complement(7469..>9507)
                     /locus_tag="VIN75_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972784.1"
                     /translation="RASSSIHVTENRMRLAPGTIHFLTLGVASLAMLSACGGGSDAAP
                     TPPAPTPSATHLTGSAAVGAPMTQGTLRVIDATGAVVAHDIPINADGTYDAGVLTGTG
                     PWRIEACGYTGANYGCIYSVAQAAGTANVTPLTSAMITLATGQKADAVMSAASAPGAS
                     ALDAAQDQLRSGLAGTLSDAGVPSDIDFTTGALQAGSRSGYDRVLDAVDVTTGTDNGA
                     FVQVTPRMGDGNLYMTAGSTTGTITADAGAATLPLDGLETLFKKMTAAVASAPACADP
                     TTGLASVVASDAHISMGDGTLTGGAQVSTGLCQFFASGADTGGGAIWGSQLVSPVLGR
                     CDLTGADPLCAISFAIQMPDGTIQNVGDGIGVVYRDKHWQFYGDLLPMAINANAAVQR
                     SVRVDDPGTPVQYQRALQFDIARTDGVACAVLSARNDAHTTIAVYKVFSPTVPRMSLW
                     TTDGMSNVASTDPAAGALRSGDDTWLSLPQGTAGDDVIRTFYRAGRTIDVAIYSDAAC
                     TAPATVGGRSSFAVDVAGMPPVDSAMASLPWGTLTDGTVAALEAFTVDGGHTSSFSST
                     WTFADGVTGFEQSVFCTAGSCGDGEPARLGDTRIRPGALSAVTPLKAPATHVLAGDFK
                     EYILGGRDATGMNIESEFFSCTSTPAGQMCGQGPAPIGSGGTTTGPTASHKRTH"
BASE COUNT         1281 a         3404 c         3509 g         1313 t
ORIGIN      
        1 gtctacaccg cggcggcgtc ggtcaccggc atgacgacga ccgtgacccc gtcgacgctg
       61 tcgctggccc cgggccagag cgccagcttc acggtgacga tggccccggc cgccagcacg
      121 cccaagttca cgtggcagta cggctcgctg acgtggaacg acggcgccgg ccacaacgtg
      181 cgcagcccga tccaggtggc gctcggccag tcgatctcgg cgccttccga cctcagcgcg
      241 accacggtct cgggcagccg catgttcacg atcagcaccg gctttgccgg ccacgcgtcg
      301 gcgaacaagg gcatgcgtga cgtgacgatg ggcccggcga cgacgctgac gcccaacagc
      361 aacgtcgacg ccgccgcggt ctgcaaggcc acgggtccca ccgccaacgt cgcggtctac
      421 aacttcaccg tgcccgccgg cgcgatcgtg gcgcgcttcg cgctgcgcca ggccgacgtc
      481 agcggtgcga ccgacgacaa cgacgtggcc gtggtgctgc ccagcggcgc ggtggcggcc
      541 tccgaaggcg gcacgtcgca cgagacggtg gaactgctga acccggcggc cggcaactac
      601 aaggtctgcg tggcctcgta cgccgcggcc agcgggtcgt cgacgcacca gctctcgagc
      661 tggatcgtca gtcccggcga caccgccggc ggcgcgttca acgtcgcgat gccgtccacc
      721 gtgtactcgg gcggcacgtc gacggtcggc gccgtgtggt cgggcctgtc ggccggccac
      781 cgctacatcg gcggcatcca gtacctcgac gcgtcgagcg cggtcgccgc agccacggcc
      841 ctgtccatcg acacgacgcc gggcacgccg ctggacatgc ccacgccgat cgccaacgac
      901 aaggccagcg tcaccaagaa gtaagccgga cgagcgccgc ggcgctcctg ccgcctgcca
      961 gggccgcgcg tcgcgagacg cgcggccttt ctcattgcgt gcccgtggcc gcgctcagat
     1021 cgccgagtcg gtgccgccct gccccagctg gcagctcttc ctgccatcgc tcgcgcgcgg
     1081 gcggcacacg acgctcgggt cgggccggcg catgcaatcg gaggcgaggc cgctggcctt
     1141 gttctcctcg acccgggcct gcgccagcgc ctgcacggca tcctgcaacg cgtgcgcatc
     1201 ggtgatcttg ctggaccacg gcagccacgt ctcggcgccg ccgcagggct tggcgccgag
     1261 tccgacggcg tggcattgcg cctggccgtc gcacgcggcg tcgccgatca gctcgacgac
     1321 gcgggcgtgc agcgcgacgg acttcggcgg ggccgcggcc gcggccgcgg gcgccgcggg
     1381 atcgaccgcg gaggcgcagc cggcgatggc caggagcgag gcgacgacga gcgtcgctcg
     1441 gcgaacagga cggcggatga tgaagttcat cggggcatgc tcgcccatcg caaggacgcc
     1501 tgcaattcgg tgaacctcgc ggcccgtcgc cttcagacga agtcgcgcac ccggtagtag
     1561 gacatcccga cggcctgcat cagcccgccg atggcctcgc cgcccgggaa cggcgggttg
     1621 acgatggggg cgaaccagtc gagctcgtcg gacctggcga gcatcttgtc ggtgaccacc
     1681 ttggccgcca cgcgcgtggg caccaggccg tggccgcaga agccctgcgc ccagaagacg
     1741 cggccgtcgc gctggcccca gtcgggcatg cggcgcatcg tgatgtcgat gtggccgccc
     1801 caggcgtagt ccatctgcac gttggccagt tgcgggaagg cgcgcgcgat gttcacgcgc
     1861 atcgcgtcct tgaggttgga cggcgtgccg ccgaggtagg tgcacttgcc gccgaacagc
     1921 agccggtggt cggccgtcat gcggaagtac tcgaggatga actggttgtc gtagaccgcg
     1981 tggttgtgcg ggatcaggct gcgcgcgacg gcctcgccca gcggcgcggt gacgccgatg
     2041 aaggtgccca cgggcagcgt gcgcgcggcc agcttcgggt cgagcttgtc gacgtaggcg
     2101 ttggtggcca gcacgagctg gtcgcagcgc acgacgtgcg ggccgacctt gacctggatg
     2161 cggtcgccct ggttctcgac gtaaccgtcc accttgctct gctcgaagat gcgcacgccc
     2221 aggccttcca ccgcgcgggc caggcccagc acgtacttga gcgggtggat gtggcagccc
     2281 tccgggtcga tgatgccggc ctggaagcgc gccgagttca ggtacttggg caggtccttc
     2341 ttctcgacca gctgcagctt gtcgtagccc cactcgttga cgcactcctc gatccagtcg
     2401 gtgaggtcgg cgacgcggcg cttgagcacg gcgacctcga ggtggccctg caccaggtcg
     2461 cagtcgatct ggtgctgcgc catgatgccg cggatgtcgg tgacggcctc gcgcgaggcg
     2521 tcgaacatgc gcttggcgcg ttcgtggccc atcacgccct tcagccgctg catgccgcac
     2581 gggaagccga tgatcatctg cccgccgttg cggcccgtcg cgccccagcc cacgcggctg
     2641 gcctcgacga tggccacgcg cttgcccgcc ttggccagct ccatcgcgga atacaggccg
     2701 aagaagccgg caccgacgac gcagacgtcg acgtccaggt tctcctgcag cgacgggcgg
     2761 ctggtctgca ggtggcgcga cgtggcggcg tagtacgagt cgacgtagtc gggcgaggaa
     2821 cggaacatgg aactctctct cgggaaggcg tggaaacgaa aaggcggccg gatcaggcga
     2881 gctcgaccag gtggacgagg tcccagtggt ggcgcgggtc ggcggcggcg tggcgctcgg
     2941 cgctctcgtg gcgcttgagc gcgacgaaca ggtcgaccag cggggcgccg aacagctcgc
     3001 gcatcagcgc gctcgcctcg agcgcgtcgc aggcctcggg cagcgagcgc ggcagcgcga
     3061 cgccggcgga ctcgcccgcg ggccgcaggc cctgctcgat gccggccagg cccatgccca
     3121 gcgtggcggc cacgaccagg taggggttcg catcgccgcc gggcaggcgg ttctcgatgc
     3181 gccggttctg cgcgctcgcc tgcgggatcc ggaacgcgac ggcgcggtcg tcgtggcccc
     3241 agctggccga cgacggcgag gcattggaca gccggatgcg gtcgaacgcc atgtcgtgcg
     3301 gcgcgaagaa cgccatcgcc gcgtcggcgt ggcgctgcag gccgccgatg aaggcgccga
     3361 gcaccggcgt gtcgcgacca tccggatgcg cgaaggcgtt cgtgtgcgcg ccggggacat
     3421 cggtgccctc gccggcgagc gctcggccgc tccggagcga gccggcgtga gccccctggg
     3481 ggcggggagc ggagcgagcc gggctgtcat gaatgctgaa gtgccagtgc atgccggtgc
     3541 cgggctggtc gaggaagggc ttgggcgcga acgtgcacag gaagccgaac tgcgcggcca
     3601 cctggcgcgc gaggcgcttg aagcggaaca ccgcgtcggc ctgggccagc ggctcgccgg
     3661 gcatgaagtt gacctcgtac tgcgagtacg cggcctcgtg cgcgtggccg cacaccggga
     3721 tgccctgcag ctggcaggcc gcgaacagcg cgtcgaagta gtcggcgaag tggccggtgc
     3781 gctccagcga gtaggcctcg caggcgcgct cgcgcgcggg agcgcctgcg cgtgacgcgg
     3841 gcgcgtgcag gctcacctgg ccgccctcct cgcgccggtc gacgaggaac agctccagct
     3901 ccggcgcgac ggtggccgcg aggccggcct gctcgaggcg cgccagcacg cggcgcaggg
     3961 cggcgcgggg cgagtattcg ctggcgcaca cggcgcggtc gtagcggccg gcgggcagcg
     4021 ggccggcggg ctcgcagatc accgccgcgg tgccggcgcg tcccggctgg gcgcgcaggg
     4081 tcgcgaggtc ggggacgagg ctgatgtcga ggtaggacgc cggcagcagc gggccgaaca
     4141 cggcctcggg ctcgccggcc gtcagcgtca ggccgaacac caccgatggg aaacggcagc
     4201 cggccatgcc cacgaagtcc tggtggccga cggacttgcc gcgcgccacc gacgtgaagt
     4261 cgccgaccgc gcattcgatg gcggtcaccg gcacgcccag ctcggcggac aggctgtcca
     4321 gcgcggcctg taccacggcg cggtggggcg cggcgagcgc agtggcggaa tggaaatcgg
     4381 acgatgcggt catgcgcgga gcttacgcag cgattggccc agtgggaagg gccacttcgc
     4441 ggcaagcggg ctgggccact tggcgtacga tcgcgcttgc ccatggcctc gaacgccccc
     4501 acccacctcg caggcgacgc gctcgtcgac tggctgcagc acgcctgggc ccaccccgcg
     4561 gcggacgagg ccgcgctgcc gatcggcgcg cgcctgaccc agcagatgcg gcgcgcgatc
     4621 ctcgagcgcg tcgtgccgcc ggcgcaccgg ctgccgtcca cccggacgct ggcgcaggcg
     4681 ctgcgcatcg cgcgcaacac ggtcgtgccg gtgtacgagc agctgcgcgc cgagggcttc
     4741 gtcgtggcag gacagggctc gggcacctac gtctgccgca tcgcgcccga ggccctgccg
     4801 gccgcggcca aggcgacgga gccggccgag gcgccgcgcg cggccagccc gctcgcgttc
     4861 tcgcgccgcg gccggcgcta ccactcgcat ccgctgcacg agttctggac gcgccagccc
     4921 ttctgcccag gccagttcga tttcgagctg ttcccgcacc ggctgtggaa ccggctgcag
     4981 caacgccagc tgcggcgcgc cgacccgatc cagctcgaac agggcgagcc cggcggcgcg
     5041 cccgagctgc gccaggcgat cgccgagcat gtgcgcgcca cgcgcggcgt gcgctgcgcg
     5101 cccgatcagg tcatcatcac cgacggcacc ggcgagtcga tcgacctcgt cgcgcgcctg
     5161 atgtgcgacc ccggcgacac cgtgctgctg gaagacccgg gctactgggc cgccacgcag
     5221 gtgctgtccg accacggcct gcggctgcgg ccgatgccgg tcgacgcgca cggcctgccc
     5281 gtgccgatgc tgcgcgccgg cgagcgcgca ccgcgcgtgg tctacctcac gccgtccaac
     5341 cagttcccca ccggcggcgc gatgccgctc gcgcgccggc tggagtggct tcgctttgcc
     5401 gcgcgccacg gcacgctgct gctggaggac gactacgaca gcgagtaccg ctatgaaggc
     5461 gcgccgttcc cgtcgctgca gggccaggac gccgcgggcc gcgtcatcta cctgggcacc
     5521 ttcagcaaga cggtgtaccc gggcatccgc gtcgccttcc tggtggtgcc gcaaggcctg
     5581 caacgcgtgt tcgccgatgc gtcggccgac ttctaccgcg acggcgacca gatcgtgcag
     5641 caggtgctgg ccgacttcat gcgcgagggc cactacgccg cccacgtgcg cacgctgcgc
     5701 cgcgaatacg cgctgcgccg cgaggcgatg ctgcgcgcgc tcgccgaggc cttgccgcgc
     5761 gagttcgcca cgcagcgcct gtccgtggtg tccggcgcgc gcggcgtgca cctgacgctc
     5821 gcgctgcccg acgacgtgga cgaccgcgcc gtcgcgcgcg ctgccatcgc gcacggcgtg
     5881 acggcgatcc cgctgtcggt ctacgcgatg cacgtgggct ggcgcggcct ggtgctgtcg
     5941 ttcgcggcca cgcccgtccc gcggatcgcg ccgctcgtcg gtacgctggc gcccgtgttg
     6001 cgcaacgcgc tcgcgcacag gccctgacta tcgagccaca cggcccgatg cacagcggcg
     6061 cactgtggcg ttgcggcccg gccatatccc cacaatgagc cacccggcgg caaagcgcgc
     6121 ggcctagaat ttttggcctg gaggtcagga aaaatgcaca ggcgcaggaa gtcgcccgcg
     6181 gagatcgccg catgacgatc gacggccgct cgctgctcgc ggtggtcgcc gcgctgtgca
     6241 tcgtcttcag catcgtcgtg ctggcgctcg agcgccgcat ccgccagacc atgccgggcc
     6301 tgcgcctgtg ggccatcgcc aacctgtttc tcggcgccgc cgcggccgcg cacatgctgc
     6361 agggccatgt gcccgtgtgg gtgccggcca tcctgggcaa ctggacgatg ctggtgggcc
     6421 gcagcacgtc cgtcgcggcg ctgcgccagt tcgaccgcca gcccgtgccc atcgcctggc
     6481 tcgtcggcgc cacggccctg ttcatgctgg gcatcgccgg cacgctggtc tggcccgagc
     6541 cgcgcgtgcg cgccatgatc atggtgggcg gcatggcggt gctggcgctg tgggcgacat
     6601 actcgctcgc ggtcggcgcg tcgcccagcg tcagccgcgt gctcacgatg gtgggcatgg
     6661 gcggctgggg cctggccaac ctctggcgcc tgtggacgac ggcgcacctg cccgagggcg
     6721 cccaggtgct caacctgagc gacccgaccg tcgccgcgta cctgtgcgcg ctggtcatca
     6781 tggacgtgtt ctgcaacatc ggcttcgtgc tgctgatcac cgaccgcatg cacgagtggg
     6841 tgctgcagct ggcgcgcacc gaccacctga ccggcgcgct gtcgcgcggc gccctctaca
     6901 cgcaggccac gcgcgacctg cagctggcgc gccgccagcg cacgtacacg gcggccttca
     6961 tcgtcgacat cgaccgcttc aagacgatca acgacagcca cggccatgcc gccggcgacg
     7021 cgatcctgcg ccacgtcgcg cagcacggcc aggcggtgct gcgctgcacc gacctgttct
     7081 gccgctatgg cggtgacgag ttcgtcgcga tcctgcccga gaccgacctg cccaccgccg
     7141 cggcgatcgc cgagcggctg cgcgtggcgg tcggcgaacc ggtgccgggc gcggcgccgc
     7201 tggccagcgg cctgtgcggc gtgacggtga gcatcggcgt ggcggcggtc gcgccgggct
     7261 gcgaggccat cgacgagttg attgcgcagg ccgacgaagc gctctacgac gccaagcacg
     7321 cgggccgcaa ccgcacgcgc gtcgccgacg aagagccggc gaccccgagc aagccgccgc
     7381 tgcgcgtgat ccagggccgc aactgaaacg gcaaaggccc ggacggtgcc gggccttgct
     7441 cagggcgggc cgccgcggcc cgcgccgttc aatgcgtgcg cttgtgcgac gcggtcgggc
     7501 cggtggtcgt gccgccgctg ccgatcgggg cggggccctg gccgcacatc tggccggccg
     7561 gcgtcgaggt gcagctgaag aactcgctct cgatgttcat gccggtcgcg tcgcggccgc
     7621 ccaggatgta ctccttgaag tcgcccgcca gcacgtgggt ggccggcgcc ttgagcggcg
     7681 tcaccgcgct cagcgcgccc ggacggatgc gcgtgtcgcc caggcgcgcg ggctcgccgt
     7741 cgccgcacga gccggcggtg cagaacaccg attgctcgaa gccggtgacg ccgtcggcga
     7801 acgtccaggt gctgctgaag ctgctggtgt ggccgccgtc gacggtgaac gcctccagcg
     7861 cggcgacggt gccgtccgtc agcgtgcccc acggcaggct tgccatcgcg gaatcgaccg
     7921 gcggcatgcc ggcgacgtcg accgcgaagc tgctcctgcc gccgaccgtg gccggcgccg
     7981 tgcacgcggc gtccgagtag atcgccacgt cgatcgtgcg cccggcgcgg tagaacgtgc
     8041 ggatgacgtc gtcgcccgcg gtgccctgcg gcagcgacag ccaggtgtcg tcgccgctgc
     8101 gcagagcgcc ggcggccggg tcggtgctcg ccacgttgct catgccgtcg gtcgtccaca
     8161 gcgacatgcg cggcacggtc ggcgagaaga ccttgtagac cgcgatcgtc gtgtgcgcgt
     8221 cgttgcgcgc gctcagcacc gcgcaggcca cgccgtcggt gcgcgcgatg tcgaactgca
     8281 gcgcgcgctg gtactgcacc ggcgtgcccg gatcgtcgac gcgcacgctg cgctggacgg
     8341 ccgcgttggc gttgatggcc atcggcagca ggtcgccgta gaactgccag tgcttgtcgc
     8401 ggtagacgac gccgatgccg tcgccgacgt tctggatcgt gccgtcgggc atctggatcg
     8461 cgaagctgat cgcgcacagc ggatcggcgc cggtgaggtc gcagcggccc agcacggggc
     8521 tgaccagttg cgagccccag atcgcgcccc cgcccgtgtc cgcgcccgag gcgaagaact
     8581 ggcacaggcc ggtggacacc tgcgcgccgc ccgtcaacgt gccgtcgccc atgctgatgt
     8641 gcgcgtcgct ggcgacgacc gaggccaggc ccgtcgtcgg gtcggcgcac gccggcgcac
     8701 tcgcgacggc ggcggtcatc ttcttgaaca gcgtctccag gccgtccagc ggcagcgtgg
     8761 ccgcgccggc gtcggcggtg atcgtgccgg tcgtgctgcc ggcggtcatg tagaggttgc
     8821 cgtcgcccat gcgcggggtg acttgcacga aggcgccgtt gtcggtgccg gtcgtcacgt
     8881 ccacggcgtc gagcacgcgg tcgtagcccg agcgcgaacc ggcctgcagc gcaccggtgg
     8941 tgaagtcgat gtctgaaggc acgccggcgt cgctgagcgt gccggccagg ccgctgcgca
     9001 gctggtcctg cgcggcgtcg agcgccgacg cgccgggcgc gctcgcggcc gacatcacgg
     9061 cgtccgcctt ctgaccggtg gccagcgtga tcatggcgct cgtcagcggc gtgacgttgg
     9121 ccgtgcccgc cgcctgcgcg acggagtaga tgcagccgta gttggcgccc gtgtagccgc
     9181 aggcctcgat gcgccagggg ccggtgccgg tcagcacgcc ggcgtcgtag gtgccgtccg
     9241 cgttgatggg gatgtcgtgc gccacgacgg cgcccgtggc gtcgatcacg cgcaacgtgc
     9301 cttgggtcat cggggcgccg accgccgcgg aaccggtcag gtgcgtcgcg ctgggcgtgg
     9361 gcgccggcgg cgtcggcgcg gcgtcggagc cgccgccgca cgcggacagc atggcgagcg
     9421 aggcgacgcc cagggtgagg aaatggatgg tgccgggggc caggcgcatg cggttctccg
     9481 tgacgtgaat tgaagacgat gcacgcc
//
LOCUS       DATCRO010000040         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_225321, whole genome shotgun sequence.
ACCESSION   DATCRO010000040 DATCRO010000000
VERSION     DATCRO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225321"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..508
                     /locus_tag="VIN75_01110"
     CDS             92..508
                     /locus_tag="VIN75_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8972785.1"
                     /translation="MQAQHLEGGCFCRALRYRLSDTPVASMVCHCRTCRRLFAAPVVG
                     WLSVPAARFAFTQGTPCTFSTTPPVTRWFCGRCGTHVAYVHADDPGWVEVATCSLDDP
                     SATPPTHHSWLSHDLPWVKFGDGLKTFPRSRHDGDA"
     gene            complement(522..1850)
                     /locus_tag="VIN75_01115"
     CDS             complement(522..1850)
                     /locus_tag="VIN75_01115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8972786.1"
                     /translation="MSFESLGMAAPLLAAAKQRGYLGPTPIQSAAIPPALAGRDVQGA
                     ARTGSGKTQAFGWPVLQRLAEAGVPPEGRKRPVRALVLAPTRELAAQIGESLREDAQH
                     LPAALKIAIAYGGVSINPQMMRLRAGADIVVATPGRLLDLVDHNAVSLSAVQTLVLDE
                     ADRLLDLGFADELQRVLALLPPRRQTLLFSATFPGAVTTLADALLHDPVRIALAGAAD
                     ASAAATEARASATAHIHQRAIAVDTASRTQLLLKLVRDHGWKRVLVFVATQYAASHVA
                     SKLHIGGLKAEAFHGDLSQGRRTQLLDDFKASRLQVLVATDLAARGIDIEQLPVVVNY
                     DLPRSADDYVHRIGRTGRAGQAGHAVSFVPAASEAHFRLIEKRQGQRVPREVIAGFEP
                     KQAAPAKGAAEAPHGTGGVKGKRPSKKDKLRAAEAARAAERAARAAKRKK"
     gene            complement(1879..2190)
                     /locus_tag="VIN75_01120"
     CDS             complement(1879..2190)
                     /locus_tag="VIN75_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972787.1"
                     /translation="MRIVAHRPSAWFLAEDDGDLLLDVNCNSGPAGFSVLARLTPAER
                     AEHAARGDAALDALAGRIQDAGPPAFAQRDVGREIGERFHACVMAWLAARRAAGDPPA
                     P"
     gene            complement(2206..3162)
                     /locus_tag="VIN75_01125"
     CDS             complement(2206..3162)
                     /locus_tag="VIN75_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8972788.1"
                     /translation="MNWLGQGVDRAGKIAWIVALAFLVVAPFVGVYPIFIAQALCFAI
                     FAIAFNLLLGYTGLLSFGHAAFFGGAAYVTAWLLQEKHLTPELAIIGGMLAAAAAGLV
                     IGAIAIRRQGIYFAMVTLALAQMLYFFFLQAPFTGGENGLQGIERGKLLGLIPLEGDL
                     ALYFFVLAVSVAVFLLVLRIVHSPYGQVLKAIRENEPRAISLGYNVDRYKLLAFVLSA
                     SLAGLGGSLKVLVLHLATLQDVRWEQSGEVILMTLLGGLGTFAGPVVGAFTVIGLQNY
                     LSDRAGSWTNVIIGCIFALCVVAFRKGVVGELLAWQQRRAKK"
BASE COUNT          474 a         1146 c         1179 g          445 t
ORIGIN      
        1 gcatttacct cgccttcacg taacagcccg catgccctgc cggacagccg atccgtacac
       61 tcccgcttcc gatcatcgac aggacgtcgc catgcaagcc cagcacctgg aaggcggctg
      121 cttctgccgc gccctccgct accgcctgtc cgacacgccg gtcgcgagca tggtctgtca
      181 ctgccgcacc tgccggcggt tgttcgcggc gccggtggtg gggtggctgt cggtgccggc
      241 cgcccggttc gcgttcacgc agggcacgcc gtgcacgttt tccaccacgc cgccggtcac
      301 gcgctggttc tgcggccgct gcggaacgca tgtcgcgtac gtgcacgccg acgacccggg
      361 ctgggtggag gtggccacct gcagcctgga cgatccgtcc gcgacgccgc cgacgcacca
      421 ttcctggctg agccacgacc tcccgtgggt gaagttcggc gacgggctga agacgttccc
      481 gcgctcgcgc cacgacggcg acgcctgaag gcgcggccgc gctacttctt gcgcttggcg
      541 gcccgcgccg cgcgctcggc ggcgcgggcg gcctcagcgg cgcgcagctt gtccttcttg
      601 ctcgggcgct tgcccttgac gccgcccgtg ccgtgcggcg cctcggccgc gcccttggcc
      661 ggcgcggctt gcttcggctc gaagccggcg atgacctcgc gcggcacgcg ctggccctgg
      721 cgcttctcga tcaggcggaa gtgcgcctcg ctggcggccg gcacgaagct caccgcgtgc
      781 ccggcctgac ccgcgcggcc cgtgcggccg atgcggtgga cgtagtcgtc ggcggagcgc
      841 ggcaggtcgt agttgacgac caccggcagc tgctcgatgt cgatgccgcg tgcggccagg
      901 tcggtggcca ccagcacctg caggcgcgag gccttgaagt cgtccagcaa ctgcgtgcgc
      961 cggccctggc tcaggtcgcc gtggaaggcc tccgccttga ggccgccgat gtgcagcttg
     1021 gacgcgacgt ggctggccgc gtactgcgtc gccacgaaca cgagcacgcg tttccagccg
     1081 tggtcgcgca ccagcttgag cagcagctgg gtgcgggaag ccgtgtcgac ggcgatcgcg
     1141 cgttggtgga tgtgcgcggt ggccgaggcc cgcgcctcgg tcgcggcggc gctcgcgtcg
     1201 gcggcgccgg ccagcgcgat gcgcacgggg tcatgcagca gcgcgtcggc cagcgtggtg
     1261 acggcgcccg ggaaggtggc cgagaacagc agcgtctggc ggcgcggcgg cagcagggcg
     1321 agcacccgct gaagctcgtc ggcgaagccg agatcgagaa ggcgatcggc ctcgtccagg
     1381 accagcgtct gcacggccga cagcgacacg gcgttgtggt cgacgagatc gagcaggcgg
     1441 ccgggcgtgg cgacgacgat gtcggcgcct gcgcgcagcc gcatcatctg cgggttgatc
     1501 gacaccccgc cgtacgcgat cgcgatcttc agcgcggcgg gcaggtgctg cgcgtcctcg
     1561 cgcagcgact cgccgatctg cgccgcgagt tcgcgcgtgg gcgcgagcac gagcgcgcgc
     1621 acggggcgct tgcggccttc gggcggcacg ccggcctcgg ccaggcgctg cagcaccggc
     1681 cagccgaaag cctgcgtctt gcccgagccg gtgcgggcgg cgccttgcac gtcgcgcccc
     1741 gcgagcgcgg gcgggatggc ggcagactgg atgggcgtgg ggccgagata gccgcgctgc
     1801 ttcgcggcgg cgagcagggg ggcggccatg cccagggatt cgaaactcat gcccgtattg
     1861 gacacgagtc gggcgacgtc agggcgccgg ggggtcgcca gcggcgcgcc gggccgccag
     1921 ccaggccatc acgcaggcgt ggaagcgctc gccgatctcg cggcccacgt cgcgctgtgc
     1981 gaatgcgggc ggccccgcgt cctggatccg cccggccagc gcgtcgagcg ccgcgtcgcc
     2041 gcgggcggcg tgctcggcgc gttcggccgg tgtcaggcgc gccagtacgc tgaagccggc
     2101 ggggccgctg ttgcagttga cgtccagcag caggtcgccg tcgtcttccg cgaggaacca
     2161 tgccgacggg cgatgggcga cgatgcgcat cgcctcaggc tgccgtcact tcttcgcgcg
     2221 gcgctgctgc cacgccagca actctccgac cacgcccttg cggaacgcca ccacgcacag
     2281 cgcgaagatg cagccgatga tcacgttcgt ccacgagccg gcgcggtcgg acaggtagtt
     2341 ctgcaggccg atcaccgtga aggcgccgac gaccgggccg gcgaaggtgc ccaggccgcc
     2401 cagcagcgtc atcaggatga cctcgccgga ttgttcccag cgcacgtcct gcagcgtggc
     2461 caggtgcagc accagcacct tgagcgagcc gcccaggccg gccagcgagg ccgacagcac
     2521 gaacgcgagc aatttgtagc ggtcgacgtt gtagcccagc gagatggcgc gcggctcgtt
     2581 ctcgcggatt gccttgagca cctggccgta gggcgagtgc acgatgcgca gcaccagcag
     2641 gaacacggcc acggacaccg ccagcacgaa gaagtacagc gccaggtcgc cctcgagcgg
     2701 gatcaggccc agcagcttgc cgcgctcgat gccctgcagg ccattctcgc ctccggtgaa
     2761 cggcgcctgc aggaagaaga agtacagcat ctgcgccagc gcgagcgtga ccatcgcgaa
     2821 gtagatgccc tggcgccgga tggcgatcgc gccgatcacg aggcccgcgg ccgcggcggc
     2881 gagcatgccg ccgatgatcg ccagctcggg cgtcaggtgc ttttcctgca gcagccaggc
     2941 cgtgacgtag gcggcgccgc cgaagaaggc cgcatgcccg aacgacagca ggcccgtgta
     3001 gccgagcagc aggttgaacg cgatggcgaa gatggcgaag cacagcgcct gcgcgatgaa
     3061 gatggggtag acgcccacga acggggcgac caccaggaac gccagcgcca cgatccaggc
     3121 gatcttgccg gcgcgatcca cgccttggcc gagccagttc atcacttctc cttcccgaac
     3181 agcccggcgg gccggatcat gagcacgatg gccatgatga agaacacgac gacgttcgac
     3241 gcct
//
LOCUS       DATCRO010000041        11447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_34108, whole genome shotgun sequence.
ACCESSION   DATCRO010000041 DATCRO010000000
VERSION     DATCRO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34108"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VIN75_01130"
     CDS             <1..594
                     /locus_tag="VIN75_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972789.1"
                     /translation="RRYRRRAVLRAIAAASALWAGVLALLALLSGCAGLLGSHDVDIS
                     ESQLTLLLARQFPMERKVLEVVDLSITNPQLTLIPQGNRVGTELDVAAVDRLFGNTAN
                     AHIKLDYGLRFQPSDHTIRMTQVRVRELTLASGSSNLHGAAQRIGGLVAEGALENLVI
                     YRMKPAQADEMDRLNLVASPITVTPQGLHMTVSPRTN"
     gene            695..1534
                     /locus_tag="VIN75_01135"
     CDS             695..1534
                     /locus_tag="VIN75_01135"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY8972790.1"
                     /translation="MDTLLLIKAAIMGIVEGLTEFLPVSSTGHLILTEALLKPGVDEN
                     VMHAFEVAIQPGAILAILIVYWQLITRTLGTLGSSPVSRRLVSNILIGFLPAAVIGFA
                     IHKQITRYLFNGPVVAGAFIVGGLIILLVESRKGREPRVTDINQMTAMDALKVGFCQC
                     FGMIPGTSRSGSTIIGGLLVGLSRPVATEFSFFLAIPTLIGASVLEVFKVRHELASNA
                     HEYLPMFAVGFIVSFLAAWVCVRWLLRYVATHTFIPFAWYRIAFGIVVLATWMTHMVT
                     WES"
     gene            complement(1686..2309)
                     /gene="trmB"
                     /locus_tag="VIN75_01140"
     CDS             complement(1686..2309)
                     /gene="trmB"
                     /locus_tag="VIN75_01140"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY8972791.1"
                     /translation="MGSGQMKALETLGPLYVLPWQPAPLDFDRAFGRHAPVILEIGFG
                     MGGATAEIAAARPQDDFIGIEVHTPGVGALLKRIEERGLTNLRLVQHDAVEVLRDMIP
                     PASLAGIHVYFPDPWHKKKHNKRRLIQAPFVKELSARLAPGGYLHCATDWQPYAEQML
                     EVLGAEPTLANTADGYAERPDWRPLTKFENRGIKLGHGVWDLVFTKR"
     gene            2589..4073
                     /gene="dacB"
                     /locus_tag="VIN75_01145"
     CDS             2589..4073
                     /gene="dacB"
                     /locus_tag="VIN75_01145"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY8972792.1"
                     /translation="MRRLLGAGAVLASPLAWAIQSPGVASHGSGPRRAVTAEQTGEAL
                     LRASGLPLASFGAYVRPVDAVSPLVAVNAEASYAMASTTKVVTSLAALDLLGVNFRWR
                     TFAFLQGALVDGTLLGDLLIVGGGDARLTSAELREWFAQLHAKGLKRIAGNIVLDRYA
                     FDLTEGDFANTPPPSTGRAYHVRPDAFAIDEGLLHVGIDPGSPGRAGKAAVSLEPPLA
                     GVRIVNKVGMRGGCTAGARLVEDAAGSHVDVFGSWGNTCGHRELELVPEAPALFTTRA
                     LAGLWAEAGGSLSGTVIERPRQVGASLLPNGPDGKPQLPWAVHLSQPLPVVLHDMNKS
                     SNNVTARHLMLALARGFPLRAATLPEAQARMHEWLLRQGLAAGDVQVENGSGLSRGER
                     AKPRALAQLLANAWHAPRLREFLLSLPVAGMDGTLQHRMQGTAAEGHAFLKTGTLLDT
                     RALAGYVQAASGRMYAVAMMVNHPDAARATPALDRVIEWIAKSC"
     gene            complement(4193..5209)
                     /locus_tag="VIN75_01150"
     CDS             complement(4193..5209)
                     /locus_tag="VIN75_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8972793.1"
                     /translation="MRASRPLRRSLLLAALASAALATQAAPVTLLNVSYDVAREFYKD
                     YDAAFVAHWKQAGGEAIVVNQSHGGSSKQARSVLDGLEADVVTMNQATDIDVLADRGQ
                     LLPANWAARLPDNAAPTTSVPVILVKKGNPKGLHDWADLARPGISVVIPNPKTSGNGR
                     YTYLAAWGSVLKAGGTPAQAREQVAKMFANVPVFDGGGRAATTTFAQRGIGDALVTFE
                     SEVELIKAEFGDNFDAVYPKRTILAENPVSVVDKVVDRKHTRKEAEAYLQWLYSGEGQ
                     EIAARHHLRPRDAKVLAAHAKDFPKVDTFTVDELFGGWKTAQKTHFDDGGTYDQILAA
                     TRRQ"
     gene            complement(5333..6550)
                     /locus_tag="VIN75_01155"
     CDS             complement(5333..6550)
                     /locus_tag="VIN75_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8972794.1"
                     /translation="MSSPAATLPRPLQANRTSFRVLGAISTAHMINDMMQSLIVAIYP
                     VLKGEFALSFTQVGLITLTYQLTASLFQPLVGLYTDRRPVPYSLPVGMTFTLSGLLML
                     AWSPDFAVVLLAAALVGTGSSIFHPESSRVARMASGGQHGLAQSLFQVGGNTGSALGP
                     LVAAAVIVPRGKGSVAWFGLAALLGIVLLLQVSRWYGAHRKAAAKGALRAHTATFGRG
                     RVAFVVAVLLVLIFSKYFYIASISSFYTFYLIEKFGLSVQSAQMHLFLFLAASAAGTL
                     VGGPVGDRIGRKPVIWGSILGVAPFALALPHVGLAWTTTFTILIGLILSSAFSAILVY
                     AQELIPGKVGAVSGLFFGFAFGMGGLGAAALGLLADRTSIDYVYQVCAFLPLLGVVAI
                     LLPDVRKPSRARA"
     gene            6671..7459
                     /locus_tag="VIN75_01160"
     CDS             6671..7459
                     /locus_tag="VIN75_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8972795.1"
                     /translation="MTAPADSVYRPVAPDERHVDALPVVTKATDYPAGHVIELHHHAR
                     GQLVYADHGVMMVAGDGGQWIVPPTRAIWMPPGVSHRIRCIGVVHMRSIYVRPDAAVH
                     LPGKAQAVGVSGLLAELIRAAMQVPLPYQADSRDGRLMRLLLDELHALPVLPLYLPQP
                     ADERLRRICERIADQPDDTSTLADWARTLEIDVKTIQRLFARETGMTFGQWRQQARLL
                     HGLERLAVGDKVVDVALALGYDSPSAFAAMFKRQFGEPPSAFYR"
     gene            complement(7566..8075)
                     /locus_tag="VIN75_01165"
     CDS             complement(7566..8075)
                     /locus_tag="VIN75_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972796.1"
                     /translation="MKASTTLCLAALLALAACDRHESDAPPPPEPAASDNSAFLEGGH
                     PAEQAAPESAQASAAAPHVDRGPPPEVPAIVRDGVRYAQGDQGRDPGLPQASGMLVAS
                     DDFSGKRLWTLAVYSEGADARRTPDARWVFFKSMAFDPDGRLRIVDEDDKAYLVDVRR
                     RTSTPVAAR"
     gene            8233..8973
                     /locus_tag="VIN75_01170"
     CDS             8233..8973
                     /locus_tag="VIN75_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972797.1"
                     /translation="MPLSRLLVSASLAALAALGGCAQSYIVPGAKADLQAFAPVSIQE
                     GFAAKPSAPFPAAIALVRVQGPTYANFHLGPSAGPAGSRYSVVTTREVEDDAQFDRLG
                     KLPQVDGVTGLNRMLLPEHPGSEKDLREAAARVHADLMLVYTFDTAFFNEDQATPLTA
                     VSLGFLPTRRIAVSTTASALLVDTRTGYVYSTYESTKRSDTRASVWGSQDAADAARRD
                     NERAAFGELVESVAGSWPKLLDRYARKA"
     gene            complement(9023..9096)
                     /locus_tag="VIN75_01175"
     tRNA            complement(9023..9096)
                     /locus_tag="VIN75_01175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9062..9064),aa:Gly,seq:ccc)
     gene            complement(9163..10131)
                     /locus_tag="VIN75_01180"
     CDS             complement(9163..10131)
                     /locus_tag="VIN75_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY8972798.1"
                     /translation="MLGKRITESEFRAALRQAEQAPRWLDIDADAMRGHYNRTGFQVR
                     HRLAEHPLFTADALFKLARRMPVSAVKYRFGSVPGNTHFDSSIERFRGDLTLDDALDH
                     LVEKRAYVALYNPEKDPEYGPVIEGLLAEIGLAARPYERLFNWYSTYVFISAHDAVTP
                     YHMDREMNFLLQIAGRKTVQLWDPRDDAVMRPEQRDHLFSFDADARPAWHEGLPARAR
                     VFELEPGLGVHHPFIAPHLVTTGQNFSISLAITYRTPHSDVMSDAHAFNDRMRPYGLG
                     RIPVGSHPVVDVAKAYAMRGVRAARQALHRGERRDKDSGEPATGRS"
     gene            10413..11306
                     /locus_tag="VIN75_01185"
     CDS             10413..11306
                     /locus_tag="VIN75_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8972799.1"
                     /translation="MGNPYQVICLSAVAPDLFTSSYGPFVVNPCKDLGGVADALHDSA
                     CDALLIEVRTPADYDRLLAWPGLSHAVMDVAVVVVAPEPTPAQCIKLLQMGVQDVLAK
                     RHASDDSLGRVLRVAIERKRMDASARRAFSTDLTTGLPTHAQLLEHMTHLLALREREP
                     AAMALIVLRLEGFRAAELSLGAEAANVLRRKAAVRLRAALRASDVVASLGSDMYGVLL
                     AWIDAEADAEGVARKLLASLKQPFSVAGQDLPVMGTVGIGQYPAHGKDATSLMRRAVG
                     QASNGNVSEQGHVVAGAANDD"
BASE COUNT         1625 a         4029 c         4076 g         1717 t
ORIGIN      
        1 cggcgatacc ggcggcgcgc cgtgctgcgc gcgatcgcgg cggcgagcgc gctgtgggcc
       61 ggcgtgctgg cgctgcttgc gctgctgtcg ggctgcgccg gcctgctcgg ctcgcacgac
      121 gtggacatca gcgaatcgca gctgacgctg ctgctggcgc gccagttccc gatggagcgc
      181 aaggtgctgg aggtggtcga cctgtccatc accaatccgc agctcacgct gatcccgcag
      241 ggcaaccgcg tcggcacgga gctcgacgtg gccgcggtcg accggctgtt cggcaacacc
      301 gccaacgcgc acatcaagct cgactacggc ctgcgcttcc agccgtccga ccacacgatc
      361 cgcatgaccc aggtgcgcgt gcgcgagctg acgctggcca gcggctcgag caacctgcat
      421 ggcgccgcgc agcgcatcgg cgggctggtg gccgaaggcg cgctggagaa cctggtgatc
      481 tatcgcatga agcccgcgca ggccgacgag atggaccggc tgaacctcgt ggcctcgccg
      541 atcaccgtga cgccgcaggg cctgcacatg acggtctcgc cgcgcacgaa ctgacgggtt
      601 cgcgcaagca ggacaggccc gcgtcacccg ggccgcgtat catcgcgcgc ttctagcaag
      661 cacgggccgc gggcggcctc acggagcgtc gagcgtggat acgttgttgt tgatcaaggc
      721 tgccatcatg ggcatcgtcg agggactcac cgagttcctg ccggtgtcgt ccacgggcca
      781 cctgatcctg accgaggcgc tgctcaagcc cggcgtcgac gagaacgtga tgcacgcgtt
      841 cgaggtcgcc atccagcccg gcgcgatcct ggccatcctg atcgtctact ggcagctcat
      901 cacccgcacg ctgggcacgc tcggctccag ccccgtctcg cggcgcctgg tgtccaacat
      961 cctgatcggc ttcctgccgg ctgcggtgat cggcttcgcg atccacaagc agatcacccg
     1021 gtacctgttc aacgggcctg tcgtggccgg cgcgttcatc gtcggcggcc tgatcatcct
     1081 gctggtggag agccgcaagg gccgcgagcc gcgcgtcacc gacatcaacc agatgaccgc
     1141 catggatgcg ctcaaggtcg gcttctgcca gtgcttcggc atgatccccg gcaccagccg
     1201 ttcgggctcg acgatcatcg gcggcctgct ggtgggcctg tcgcgcccgg tggccaccga
     1261 gttcagcttc ttcctggcga tcccgacgct gatcggcgcg agcgtgctgg aagtgttcaa
     1321 ggtgcgccac gagctggcct ccaacgcgca cgagtacctg ccgatgttcg cggtcggctt
     1381 catcgtgtcg ttcctcgcgg cgtgggtctg cgtgcgctgg ctgctgcgct acgtcgccac
     1441 gcacaccttc atcccgttcg cctggtaccg catcgccttc ggcatcgttg tgctcgcgac
     1501 gtggatgacc cacatggtga cctgggaaag ttgagccccc acggtcgctc gcgctccctg
     1561 ccccccgagg gggccggccg cgagcggccc ggcggagccg gttccgccgc tcccttgaac
     1621 tttcggtcgg cgggtgagcg gccgcgccgg caagagctgg gaaccaggcc gtcctgccgc
     1681 gcgattcagc gcttggtgaa caccaggtcc cacaccccgt ggcccagctt gatgccgcgg
     1741 ttctcgaact tcgtcagcgg ccgccagtcg gggcgctcgg cgtagccgtc ggcggtgttg
     1801 gccagggtgg gttccgcgcc gagcacttcc agcatctgtt cggcgtaggg ctgccagtcg
     1861 gtggcgcagt gcaggtagcc gccgggtgcc aggcgcgcac tcagctcctt gacgaacggc
     1921 gcctggatca gccggcgctt gttgtgcttc ttcttgtgcc acgggtcggg gaagtacacg
     1981 tggatgccgg ccagcgaggc gggcgggatc atgtcgcgca ggacctcgac ggcgtcgtgc
     2041 tggaccagcc gcaggttggt caggccgcgc tcctcgatgc gcttgagcag cgcgcccacg
     2101 cccggggtgt gcacctcgat gccgatgaag tcgtcctgcg gccgtgccgc ggcgatctcc
     2161 gccgtcgcgc cgcccatgcc gaagccgatc tccaggatca ccggcgcgtg gcggccgaag
     2221 gcgcggtcga agtccagcgg cgcgggctgc cagggcagca cgtacagcgg cccgagcgtc
     2281 tccagcgcct tcatctggcc gctgcccatg cggccggcgc gcagcacgaa gctgcggatg
     2341 gcgcggcgcg gatggccgga ggacgtttcc gatgcggcgg cgctgccgtc ttgcgaaggc
     2401 ggcgtgcccg gggcgtgggg atctggggtc atgctggaca ggaagtggcg aaggccacga
     2461 gtagggtagc tccccgggcg ttatttggta tttctcggcc gggtgcccgc ggctagtctc
     2521 gcgggatgcg gcattttgcc cgaaccccct gcgacctgga tgcgccggac ggccgtcgcc
     2581 gtgcggcgtt gcggcgtctg ctgggcgcgg gcgccgtgct ggcgtcgccg ctcgcctggg
     2641 cgatccagtc gccgggcgtg gcatcgcacg gttcgggacc acgccgcgcc gtcacggccg
     2701 agcagacggg cgaagcgctg ctgcgcgcaa gcggcctgcc gctggccagc ttcggcgcgt
     2761 acgtgcggcc ggtcgacgcg gtctcgccgc tggtggcggt caatgccgag gccagctacg
     2821 cgatggcgtc gacgaccaag gtggtcactt cgctggccgc actcgatctc ctgggcgtca
     2881 acttccgctg gcgcaccttc gccttcctgc agggggcgct ggtcgacggc acgctgctgg
     2941 gcgacctgct gatcgtcggc ggcggcgacg cacgcctgac ctcggccgaa ctgcgcgagt
     3001 ggttcgccca gctgcacgcc aagggcctga agcgcatcgc cggcaacatc gtgctcgacc
     3061 gctacgcctt cgacctgacc gagggcgact tcgccaacac gccgccgccc agcacgggcc
     3121 gcgcttacca cgtgcgaccc gacgcgttcg cgatcgacga aggcctgctg cacgtgggca
     3181 tcgacccggg cagcccgggc cgggccggca aggcggccgt ctcgctcgag ccgccgctgg
     3241 cgggcgtgcg catcgtcaac aaggtgggca tgcgcggcgg ctgcacggcg ggcgcgcggc
     3301 tcgtcgagga cgccgcgggc tcgcacgtcg acgtcttcgg ctcctggggc aacacctgcg
     3361 gccatcgcga gctggagctg gtgcccgagg cgccggcgtt gttcaccacg cgcgcgctgg
     3421 ccggcctgtg ggccgaagcc ggcggctcgc tgagcggcac cgtcatcgag cggccgcgcc
     3481 aggtcggcgc gtccctgctg cccaacggcc ccgacggcaa gccgcagctg ccgtgggccg
     3541 tgcacctgtc gcagccgctg ccggtggtgc tgcacgacat gaacaagtcc agcaacaacg
     3601 tcaccgcgcg ccatctgatg ctggcgctcg cgcgcggctt cccgctgcgc gcggccacgc
     3661 tgcccgaggc gcaggcgcgc atgcacgagt ggctgttgcg ccagggcctg gccgcgggcg
     3721 acgtgcaggt cgagaacggc tcgggcctgt cgcgcggcga gcgcgccaag ccgcgcgcgc
     3781 tggcgcagct gctggccaac gcctggcatg cgccgcgctt gcgcgagttc ctgctgtcgc
     3841 tgcccgtggc cggcatggac ggcacgctgc agcaccgcat gcagggcacc gccgccgagg
     3901 gccacgcgtt cctgaagacg ggcacgctgc tcgacacccg cgcgctggcc ggctacgtgc
     3961 aggcggccag cggccggatg tacgcggtcg cgatgatggt caaccacccc gacgccgccc
     4021 gcgcgacgcc ggcgctcgat cgcgtcatcg agtggatcgc gaagtcctgc tgaccgccag
     4081 gggtggggtc aggtcttgaa tcgctgtggg gtcaggtctt gaatcgtgca gtcctcgggc
     4141 gtccgcgccg gatccgggtg cacgattcaa gacctgaccc cttcggagag gcctactggc
     4201 gcctggtcgc cgccaggatc tggtcgtagg tgccgccgtc gtcgaagtgc gtcttctgcg
     4261 ccgtcttcca gccgccgaac agctcgtcga cggtaaaggt gtcgaccttg ggaaagtcct
     4321 tggcatgcgc cgccagcacc ttggcgtcgc gcgggcgcag gtggtgcctg gcggcgatct
     4381 cctggccttc gccggaatac agccactgca ggtaggcttc ggcctccttg cgcgtgtgct
     4441 tgcggtcgac gaccttgtcg accaccgaga ccgggttctc cgcgaggatc gtgcgcttgg
     4501 ggtacacggc gtcgaagttg tcgccgaact cggccttgat cagctcgacc tcgctctcga
     4561 acgtgaccag cgcgtcgccg atgccgcgct gcgcgaaggt ggtggtggcc gcgcggccgc
     4621 cgccgtcgaa caccggcacg ttggcgaaca tcttcgcgac ctgctcgcgc gcctgcgcgg
     4681 gcgtgccgcc cgccttgagc acgctgcccc aggccgcgag ataggtgtag cggccgttgc
     4741 ccgaggtctt cgggttcggg atgacgacgc tgatgccggg cctggccagg tccgcccagt
     4801 catgcaggcc cttggggttg cccttcttca cgaggatcac cggcaccgat gtcgtcggcg
     4861 ccgcattgtc cggcaggcgc gcggcccagt tcgccggcag cagctgcccg cggtcggcca
     4921 gcacgtcgat gtcggtggcc tggttcatcg tgacgacgtc ggcctcgagc ccgtcgagca
     4981 cgctgcgcgc ctgcttgctg gagccgccgt gcgattgatt cacgacgatc gcctcgccgc
     5041 cggcctgctt ccaatgcgcg acgaacgcgg cgtcgtagtc cttgtagaac tcgcgcgcca
     5101 cgtcgtagct cacgttgagc aaggtgaccg gcgcggcctg cgtggccaag gcggcggacg
     5161 cgagcgccgc gaggagcagg gaacggcgga ggggacggga cgcgcgcatg caaggcactt
     5221 tcgaaggctg gggggacagc ttcgatcgta cgggcccacg cctcacctca tcggcggcga
     5281 gcgcgcatat ccatgctcgg aattcgcatg acgcatcgcg cctgcccgcg catcacgcgc
     5341 gagcgcgcga aggcttgcgg acgtccggca gcaggatcgc gacgacaccc agcagcggca
     5401 ggaacgcgca cacttggtag acgtagtcga tgctcgtgcg gtctgccagc aggcccagtg
     5461 ccgcggcgcc gaggccgccc atgccgaacg cgaagccgaa gaacaggccc gagaccgcgc
     5521 ccaccttgcc ggggatcagc tcctgtgcgt acaccaggat ggccgagaac gccgacgaca
     5581 ggatcaggcc gatcaggatc gtgaacgtcg tcgtccaggc gagccccacg tgcggcagcg
     5641 ccagcgcgaa cggcgccacg cccaggatcg agccccagat cacgggcttg cggccgatgc
     5701 ggtcgcccac cgggccgccg accagcgtcc ccgcggccga tgcggccagg aacaggaaca
     5761 ggtgcatctg cgccgactgc accgacaggc cgaacttctc gatcaggtag aacgtgtaga
     5821 agctcgagat gctcgcgatg tagaagtact tcgagaagat cagcaccagc agcaccgcta
     5881 ccacgaaggc cacgcgtcca cgtccgaacg tggcggtgtg cgcgcgcagc gcacccttgg
     5941 cggccgcctt gcggtgggcg ccgtaccacc ggctgacctg cagcagcagc acgatgccca
     6001 gcagcgcggc gagcccgaac catgcgacgc tgcccttgcc gcgcggcacg atcacggcgg
     6061 cggccaccag cgggccgagc gcgctgcccg tgttgccgcc gacctggaac aatgattgcg
     6121 ccaggccgtg ctggccgccc gaggccatgc gcgccacgcg ggaggactcg ggatggaaga
     6181 tcgacgagcc cgtgcccacc agcgcggcgg ccagcagcac gacggcgaag tcgggcgacc
     6241 acgccagcat caggagaccg gacagcgtga acgtcatgcc caccggcagc gagtacggaa
     6301 ccggccggcg atcggtgtac aggcccacca gcggctggaa cagcgacgcc gtcagctggt
     6361 aggtgagcgt gatcaggccg acctgcgtga agctcagcgc gaattcgccc ttgagcaccg
     6421 ggtagatcgc gacgatcagc gactgcatca tgtcgttgat catgtgagcc gtgctgatcg
     6481 cgcccagcac gcggaacgag gtccggttgg cctggagcgg tcggggcagg gtggcggctg
     6541 ggctggacat ggcttgatgg gggtcagtgg gtgtgcgaag actcgatgct aggaataaca
     6601 tcgatgcctg tctcgcgccg ttcgggcttc gatccgcgcg aaaaggacat cactcttcga
     6661 tcccccgccc atgaccgccc cggccgattc cgtctaccgc cccgtcgcgc ccgacgagcg
     6721 ccatgtcgac gcgctgcccg tcgtcaccaa ggcgaccgac tacccggccg gccacgtcat
     6781 cgagctgcac caccacgccc ggggccagct cgtctacgcc gaccacggcg tgatgatggt
     6841 ggccggcgac ggcggccagt ggatcgtgcc gccgacgcgc gcgatctgga tgcccccggg
     6901 cgtatcgcac cgcatccgct gcatcggcgt cgtgcacatg cgcagcatct acgtgcggcc
     6961 ggacgcggcg gtgcacctgc ccggaaaggc gcaggcggtg ggcgtctcgg gcctgctggc
     7021 cgagctgatc cgcgccgcca tgcaggtgcc gctgccctac caggccgact cgcgcgacgg
     7081 ccgcctcatg cgcctgctgc tggacgaact gcacgccttg ccggtacttc cgctgtacct
     7141 gccgcagccc gccgacgaac gcctgcgccg catctgcgag cgcatcgccg accagcccga
     7201 cgacacctcc acgctggccg actgggccag gacgttggag atcgacgtca agaccatcca
     7261 gcgcctgttc gcgcgcgaga cgggcatgac cttcggccaa tggcgccagc aggcgcggct
     7321 gctgcacggg ctggagcggc tggccgtcgg cgacaaggtg gtcgacgtcg cgctggcgct
     7381 cgggtacgac agcccgagcg cgttcgccgc catgttcaag cggcagttcg gggagccgcc
     7441 cagcgcgttc tatcgctgac cgacggcggc cccgagcggc cgcggccttc atatgaccgt
     7501 ggtcgctccg cccacgctcg gctgcgcggc caacacctgc caggccgtca ccccggccaa
     7561 cggagctacc tggcggcgac cggcgtcgac gtgcgccgcc gaacgtcgac caggtaggcc
     7621 ttgtcgtcct cgtcgacgat gcgcagccgg ccgtccggat cgaacgccat cgacttgaag
     7681 aagacccagc gcgcgtcggg cgtgcggcgt gcgtccgcgc cttcggagta gaccgccagc
     7741 gtccacaggc gcttgccgct gaagtcgtcc gacgcgacca gcatgccgct ggcctgcggc
     7801 agtccggggt cgcgcccttg gtcgccctgg gcgtagcgca cgccgtcgcg cacgatggcc
     7861 ggcacctccg gcggcggccc gcggtcgaca tgcggcgcgg ccgccgacgc ttgcgcggac
     7921 tccggcgccg cctgttccgc gggatggccg ccctcgagga aggcgctgtt gtccgacgcg
     7981 gccggctcgg gcgggggcgg ggcgtccgac tcgtggcggt cgcaggcggc cagcgccagc
     8041 agggcggcga ggcacagggt cgtcgaggct ttcatgcgcg ggaatcgtga ggggacggcg
     8101 tgggtcgacg cgcatggtac cgggcgatcc cgctcgacag gacgggcgtt tgtccgacat
     8161 cgccgccgcc cgcgtcgccc taagctcctg gcggtcggca agccgccgtc gccgtcccgt
     8221 ggaatcgtcg ccatgccgct gtcccgcctg ctcgtgtccg ccagtctcgc cgccctggcc
     8281 gccctcgggg gctgcgccca gagctacatc gtgcccggcg ccaaggccga cctgcaggcg
     8341 ttcgcgccgg tgtcgatcca ggagggcttc gcggccaagc cgagtgcgcc gttcccggcc
     8401 gccatcgcgc tcgtgcgcgt gcaggggccc acgtacgcca acttccacct cggcccctcg
     8461 gccggccccg caggcagccg ctacagcgtg gtgaccacgc gcgaggtgga ggacgacgcg
     8521 cagttcgacc gccttggcaa gctgccgcag gtcgacggcg tcacgggcct gaaccgcatg
     8581 ctgctgcccg agcatcccgg cagcgagaag gacctgcgcg aggccgcggc gcgcgtgcac
     8641 gccgacctga tgctggtcta taccttcgac accgcgttct tcaacgagga ccaggccacg
     8701 ccgctgacgg ccgtctcgct gggcttcctg ccgacgcgca ggatcgcggt cagcacgacg
     8761 gcgtcggcct tgctcgtcga cacccgcacg gggtatgtct actcgaccta cgaatcgacc
     8821 aagcgcagcg acacgcgcgc cagcgtctgg ggcagccagg acgcggccga cgcggcgcgc
     8881 cgcgacaacg aacgcgcggc gttcggcgaa ctcgtggagt cggtggccgg gtcgtggccg
     8941 aagctgctgg atcgctacgc gcgcaaggcc tgaggcctcg caccccgcct ggcggcgcac
     9001 tgcggccgcg cgatcaggga tgtggagcgg gtgatgggaa tcgaacccac gtatgaagct
     9061 tgggaagctg ccgttctacc attgaactac acccgcagtt cgcgacggat tctagccgag
     9121 ccgcgaaggg cccgccgcgg gtgtcggcgg ctgcgccgcg gttcaggagc ggccggtcgc
     9181 gggctcgccg ctgtccttgt cgcgccgctc gccgcgatgc agcgcctgcc tggcggcgcg
     9241 cacgccgcgc atggcgtacg ccttggcgac gtcgaccacc gggtggctgc ccacggggat
     9301 gcggcccagg ccgtaggggc gcatgcgatc gttgaaggcg tgggcgtcgc tcatgacgtc
     9361 cgaatgcggc gtgcggtacg tgatggccag cgagatcgag aaattctggc ccgtggtgac
     9421 caggtgcggt gcgatgaacg ggtgatgcac gcccaggccc ggctccagct cgaagacgcg
     9481 ggcccgcgcg ggcaggccct cgtgccaggc ggggcgcgcg tcggcgtcga acgagaacag
     9541 gtggtcgcgc tgctcgggcc gcatgaccgc gtcgtcgcgc gggtcccaca gctggaccgt
     9601 cttgcggccg gcgatctgca gcaggaagtt catctcgcgg tccatgtggt acggcgtgac
     9661 ggcgtcgtgc gccgagatga acacgtaggt cgagtaccag ttgaacaagc gctcgtaggg
     9721 ccgtgcggcg aggccgatct cggccaggag gccctcgatc acgggcccgt actcggggtc
     9781 cttctccggg ttgtagaggg cgacgtacgc gcgcttctcg acgaggtggt cgagcgcatc
     9841 gtccagcgtc aggtcgccgc ggaagcgttc gatggacgaa tcgaaatgcg tgttgccggg
     9901 cacgctgccg aagcggtact tgacggcgga gaccggcatc cggcgcgcga gcttgaacag
     9961 cgcgtcagcg gtgaacagcg ggtgttcggc caggcggtgc cgcacctgga agccggtgcg
    10021 gttgtagtgg ccgcgcatcg cgtcggcgtc gatgtcgagc cagcgcggcg cctgttcggc
    10081 ctgccgcagc gcggcgcgga attcggattc ggtgattcgt ttgcccagca cgatgcggcc
    10141 cccctcgtcg tctccggccc cccctgctga tgccattctc gcgacgctcg tcgccattgt
    10201 ttccgctggg ccgcagcggt gtcaccggtg tacccggaac tagggggagg cggctgtcgg
    10261 cagactgaac gcgtagccgg tccgaggcgt ttgcaaaggg gctatcccgg cgtgccggcc
    10321 gaaagccttc ccatggcccg attcagtgtt tccatgtctg tgcactgcag caaatccggg
    10381 gcatcatgca agccaatcca acgttggcgc ccatgggaaa tccgtatcag gtcatctgtc
    10441 tcagtgccgt cgcgccggat ctgttcacct cgtcctacgg gccgttcgtg gtcaatccgt
    10501 gcaaggacct gggcggcgtc gccgatgcct tgcacgacag cgcctgcgac gccttgctca
    10561 tcgaggtcag gacgccggcg gactacgaca ggctgctggc ctggcccggc ctgtcgcacg
    10621 cggtgatgga cgtcgcggtg gtcgtcgtcg cgcccgagcc cacgcccgcc cagtgcatca
    10681 agctgctgca gatgggcgtg caggacgtgc tggccaaacg ccacgccagc gacgattcgc
    10741 tcggccgcgt gctgcgcgtg gccatcgagc gcaagcgcat ggacgccagc gccaggcgcg
    10801 cgttctccac cgacctgacc accggcctgc ccacgcacgc gcagttgctg gaacacatga
    10861 cccacctgct ggcgctgcgc gagcgcgagc ccgcggcgat ggcgctgatc gtgctgcggc
    10921 tggaaggttt ccgcgcggcc gaactgtcgc tcggcgccga ggccgccaac gtgctgcgcc
    10981 gcaaggccgc cgtgcgcctg cgcgcggccc tgcgcgcgag cgacgtcgtg gcgtcgctgg
    11041 gctcggacat gtacggcgtg ctgctggcct ggatcgacgc cgaggccgac gccgagggcg
    11101 tcgcgcgcaa gctgctggcc tcgctcaagc agccgttcag cgtcgccggg caggatctcc
    11161 cggtgatggg caccgtgggc atcggccagt atccggcgca cggcaaggac gcgacgtcgc
    11221 tgatgcgccg cgcggtgggc caggcgtcca acggcaacgt gtccgagcag ggccacgtcg
    11281 tggccggcgc ggccaacgac gactgacccg cgcagggcgg gtcagcgccc gcggttcgaa
    11341 cgacccgccc gacggcctcg cgcgcgcatg acgaccgccg cggcgatcgc cgcgagcagc
    11401 accccggccg cgacgatcca tgcccacggc aggccctcgt cctgcgg
//
LOCUS       DATCRO010000042         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_146669, whole genome shotgun sequence.
ACCESSION   DATCRO010000042 DATCRO010000000
VERSION     DATCRO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146669"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2082
                     /locus_tag="VIN75_01190"
     CDS             <1..>2082
                     /locus_tag="VIN75_01190"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY8972800.1"
                     /translation="MQIIGKRHFGKKAAPPGGGGGQFPTRELFDTLLLWRPAVVLDAK
                     GEAVVGVPLNDSLTSFRIVAVVDVVQGAQSALFGTGQATIAATQDLQIVSGVPPLVRE
                     GDHYKAMFTLRNTSKAAMSASLAASAGAQPLPAQTVTIAAGAAADVSWDVDVPFNVDS
                     LAWTVAADAGNAHDRMKFAQKVAQAVPVTVQQATLMQLDKTMRVPVAPPVNALPDGTG
                     RLRGGIEVALKPKLGDGLPGVRDYFQWYPWDCFEQRASEAIGLRDTARWHALIGQISL
                     YQDEDGLLTYFPNGRARDGSDSLTAYVLAISDEATRLGYDFALPDDAKASLERGLIGF
                     VEGHVNRDFWHPAFLTKGDLDVRKLAAIEALSRYGKAQPRMLDSLQILPNQWPTGAVI
                     DWLQILDRMPAIKDHDKRVEEAQQILRARLNVQGTRLAFSTERDDSWWWLMSNGDVNS
                     VRMTLAVLDRKDWAEDVPRIVTGTLQRQQRGRWSTTVANAWGSVAMEAFSKKFETVPV
                     RGTTKAGFGVPGATSALDWSRMPAGGALPLGWPQGFGVGGNKADANLQVDHDGTGKPW
                     VTVTSKAAVPVTVPFGSGYRIAKTITPVEQKVKGVWSRGDVVRVHLDIDVQADATWVV
                     VDDPIPGGAAMLGTGLGNDDRISTGSEKDDSRGWLAYQARSFEAFRSYYRYLPKGAFS
                     LEYTFRLNNPG"
BASE COUNT          328 a          715 c          758 g          281 t
ORIGIN      
        1 gatgcagatc atcggcaagc gccacttcgg caagaaggcc gcgcccccgg gcggcggcgg
       61 cggccagttc cccacgcgcg agctgttcga caccctgctg ctgtggcgcc cggccgtggt
      121 gctcgatgcg aagggcgagg ccgtcgtcgg cgtgccgctc aacgactcgc tcacgagctt
      181 ccgcatcgtc gcggtggtcg acgtggtgca gggcgcgcag tccgccttgt tcggcaccgg
      241 ccaggcgacc atcgccgcca cgcaggacct gcagatcgtc tcgggcgtgc cgccgctggt
      301 gcgcgagggc gaccactaca aggcgatgtt cacgctgcgc aacacgtcga aggcggcgat
      361 gagcgcgtcg ctggcggcgt cggccggcgc gcagccgctg ccggcgcaga cggtcacgat
      421 cgcggccggc gcggcggcgg acgtgtcctg ggacgtggac gtgccgttca acgtcgacag
      481 cctggcgtgg acggttgcgg ccgacgcggg caatgcgcac gaccgcatga aattcgcgca
      541 gaaggtggcg caggccgtgc cggtgacggt gcagcaggcc acactgatgc agctggacaa
      601 gacgatgcgc gtgcccgtcg cgccgccggt caacgccttg ccggacggca cgggcaggct
      661 gcgcggcggc atcgaggtgg cgctcaagcc gaagctgggc gacggcctgc ccggcgtgcg
      721 cgactacttc cagtggtatc cgtgggactg cttcgagcag cgtgcctcgg aggccatcgg
      781 cctgcgcgac accgcgcgct ggcacgcgct gatcggccag atctcgctgt accaggacga
      841 ggacgggttg ctgacctact tcccgaacgg ccgcgcgcgt gacggcagcg acagcctgac
      901 cgcctacgtg ctggccatca gcgacgaggc cacgcgtctg ggctacgact tcgcgctgcc
      961 cgacgacgcc aaggccagcc tggagcgcgg cctgatcggc ttcgtcgagg gccacgtcaa
     1021 ccgcgatttc tggcatccgg ccttcctcac gaagggcgac ctggacgtgc gcaagctcgc
     1081 tgcgatcgag gcgctgagcc gctacggcaa ggcgcagccg cgcatgctgg actcgctgca
     1141 gatcctgccg aaccagtggc ccaccggcgc ggtcatcgac tggctgcaga tcctggaccg
     1201 catgccggcg atcaaggatc acgacaagcg cgtcgaggaa gcgcagcaga tcctgcgcgc
     1261 ccgcctgaac gtgcaaggca cgcggctcgc cttctccacc gagcgcgacg acagctggtg
     1321 gtggctcatg agcaatggcg acgtcaacag cgtgcgcatg acgctggcgg tgctcgatcg
     1381 caaggactgg gccgaggacg tgccgcgcat cgtcaccggc acgctgcaac gccagcagcg
     1441 cggccgctgg tcgaccaccg tggccaacgc gtgggggagc gtcgcgatgg aggcgttctc
     1501 gaagaagttc gagaccgtgc cggtgcgcgg cacgacgaag gcgggcttcg gcgtgccggg
     1561 cgcgaccagc gcgctggact ggtcgcggat gcccgccggc ggcgcgctgc cgctcggttg
     1621 gccccagggc ttcggcgtcg gcggcaacaa ggccgacgcc aacctgcagg tcgaccacga
     1681 cggcaccggc aagccctggg tcaccgtcac gtccaaggcc gcggtgccgg tgacggtgcc
     1741 gttcggcagc ggctaccgca tcgccaagac gatcacgccg gtcgaacaga aggtcaaggg
     1801 cgtgtggtcg cgcggcgacg tggtgcgggt gcacctggac atcgacgtgc aggccgatgc
     1861 gacctgggtc gtcgtcgacg acccgatccc gggcggcgcg gccatgctgg gcacgggcct
     1921 gggcaacgac gaccggatca gcaccggcag cgagaaggac gactcgcggg gctggctcgc
     1981 gtaccaggcg cgcagcttcg aggccttccg ctcgtactac cgctacctgc ccaagggcgc
     2041 gttcagtctc gagtacacgt tccgcctgaa caacccgggc ca
//
LOCUS       DATCRO010000043         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_292939, whole genome shotgun sequence.
ACCESSION   DATCRO010000043 DATCRO010000000
VERSION     DATCRO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292939"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIN75_01195"
     CDS             complement(<1..194)
                     /locus_tag="VIN75_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972801.1"
                     /translation="MSWSERQRRMLAAMGVRLFAPTAPAAAAPSPAAVAAAAEAVEPV
                     VPADTAVIERPPEAMAPATAA"
     gene            complement(191..694)
                     /gene="rimI"
                     /locus_tag="VIN75_01200"
     CDS             complement(191..694)
                     /gene="rimI"
                     /locus_tag="VIN75_01200"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8972802.1"
                     /translation="MSAVRSEFAAPRREPVLRVMTVPSLDAVLAIEVQAYTFPWTRGN
                     FIDSLAAGYLARLLLAEDGELIGYFVAMQGFEEMHLLNLTVAAHHQGRGHARRLLAEL
                     YTLSASCSATAVWLEVRESNERARALYLREGFVEAGRRRDYYPAPQGRREDALLMTRG
                     VRREGSA"
     gene            complement(691..1461)
                     /gene="tsaB"
                     /locus_tag="VIN75_01205"
     CDS             complement(691..1461)
                     /gene="tsaB"
                     /locus_tag="VIN75_01205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8972803.1"
                     /translation="MKLLAIDSATERLALAAIDDGRAPGGPLLRAACRVEPLDLDGGA
                     AASAQALPMLIALLQRAGWAFSDLDAVAFGRGPGGFTGLRTACAVAQGLAFGQDVPVV
                     PVDSLAIVAEDAGRRLGLAAGAVVWVAMDARMDEIYAGCYRDDGDAGWRAVVEPALTA
                     PDALHALWRDAPPAVVAGSALDAFGERLQTGAAQRVPVEHGRAEALGWLAAAGFGRGD
                     AVPADQALPIYLRDKVAQTTAEREAAKAARDAAAGAPR"
     gene            1623..3074
                     /gene="dacB"
                     /locus_tag="VIN75_01210"
     CDS             1623..3074
                     /gene="dacB"
                     /locus_tag="VIN75_01210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY8972804.1"
                     /translation="MSVRLRLATLAFALAAIAAPPTVSAAPAWALPPTIVAALDKAHV
                     PPDALALWVQPVDASGPSWGWNPDFGVNPASVFKLVTTSAALDTFGPAWTWKTGVHVT
                     GPVRNGVLDGSVVIVGSGDPSLVMERVWLLLRQLRDAGVRDIRGDIVLDHTAFAPSAR
                     SPADFDNAPSEPYNVLPDALLMNFRSVTLGFRPQPGGRVARVTSEPPLAGLAVAPTVP
                     LSKAACGDWRAGLRLSADDPARWRFAGSYPAACGDRNWPLAYPDPATYENRLVAALWR
                     ELGGKLAGKVHAGTLPEGATHAFDFESPQLAQIVRDINKFSNNTMAEQLFLTLASNAS
                     ATASVAQPEQARAVLSQWLHERLGQAADGVVVANGSGLARETRISARTLGALLQSDWR
                     SPAMPELMSSLPIVGVDGTLRRSKAEAGRAHLKTGSMRDVVAVAGYVLADSGRRYVLV
                     AVVNDPNAGAAHPVLDAAIDWVAHDPAQAPRTP"
     gene            complement(3156..3830)
                     /locus_tag="VIN75_01215"
     CDS             complement(3156..3830)
                     /locus_tag="VIN75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY8972805.1"
                     /translation="MKKHSIASALVATAALCGAGAAQAQVAGQVMVKLGWNKIMPKVR
                     STDLSAPALPGSTIDIKSASALFFTATYMITDDISVEALGGLPYKHDIIGTGSVQGVG
                     KIGSIHQISPTVLLQYRFMPADAPLRPYVGAGPTFAKFYGSKGSAALTAVTNPGGPPT
                     TIGGDTEWGATTEVGLNYKIDKHWFVDAAVLKTFINTKAQLSTGQTTKARLNPVAINA
                     SIGYAF"
     gene            complement(3864..5012)
                     /locus_tag="VIN75_01220"
     CDS             complement(3864..5012)
                     /locus_tag="VIN75_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8972806.1"
                     /translation="MKKFHVLTTALAAAVFLAGCGGNEAGDQKPRVAFTSMVSFGDSL
                     SDVGSYAVGAVALQGGGHYSINGIGATGLLWTNWTEFLSATLDLTAPCAAETGLNTIQ
                     AVVGVPPVPATFHDASAGGSCTNYAQGGARVTNPVGPGNALLFNAADPSTYSNAIGQL
                     TVPVQTQIANYLAHNGGKFTSDQLVTVLAGGNDIFINVAVFQATVAAGGDPTAAATAA
                     VTAMAQAGTEEAAYVNTQILANGATHVVVVNLPDVSLTPFAVGSGAQGQALVKSMTQA
                     FNSALQAGLTASANLVVVDAFSASEDQATNMAQYGLTDVSHPTCTTSSSLVCTAATLI
                     PAVAPAASGAAATAANTYEYADSVHPTPYGYRLLAELVSEQLAIKGWL"
BASE COUNT          719 a         1899 c         2013 g          767 t
ORIGIN      
        1 gcggcggttg ccggcgccat cgcctccggc gggcgctcga tcacggccgt gtcggcgggc
       61 acgaccggct cgacggcctc ggccgccgcg gccacggcgg ccggggacgg cgcggcagcc
      121 gcgggcgcgg tcggcgcgaa caggcgcacg cccatcgcgg ccagcatcct gcgttggcgc
      181 tcgctccagc tcatgcgctg ccctcgcgcc ggacgccgcg ggtcatgagc agcgcatcct
      241 cgcggcggcc ctgcggcgcc gggtagtagt cgcgccgccg gcccgcttcc acgaagcctt
      301 cgcgcaggta cagcgcgcgc gcccgttcat tgctctcgcg cacctcgagc cagaccgcgg
      361 tggccgagca gctcgcgctg agcgtgtaga gctcggccag caggcggcgc gcgtggcccc
      421 ggccctggtg gtgcgcggcc acggtcaggt tcagcaggtg catctcctcg aagccctgca
      481 tcgcgacgaa gtagccgatc agctccccgt cctcggccag cagcaagcgc gccaggtagc
      541 cggccgcgag cgagtcgatg aagttgccgc gcgtccatgg gaaggtgtag gcctgcacct
      601 cgatcgcgag caccgcgtcc agcgagggca cggtcatcac gcgcagcacc ggctcgcggc
      661 gcggcgcggc gaactcggac cgcacggcgc tcatcgcggc gctccggctg ccgcgtcgcg
      721 cgccgccttg gcggcctcgc gctcggcggt ggtctgggcg accttgtcgc gcaggtagat
      781 cggcagcgcc tggtcggccg ggacggcgtc gccgcgtccg aaaccggctg cggcgagcca
      841 gcccagcgcc tcggcgcggc cgtgttcgac gggcacgcgc tgcgcggcgc ccgtctgcag
      901 ccgctcgccg aaggcgtcca gcgcggagcc ggcgaccacc gcgggcggcg catcgcgcca
      961 gagcgcgtgc aacgcgtcgg gcgcggtgag cgcgggctcg accacggcgc gccaaccggc
     1021 gtcgccgtcg tcgcggtagc agcccgcgta gatctcgtcc atgcgcgcgt ccatggccac
     1081 ccagacgacc gcgccggcgg ccagcccgag ccggcgcccg gcgtcctcgg cgacgatggc
     1141 caggctgtcg acgggcacca cgggcacgtc ctgcccgaag gccaggccct gggcgaccgc
     1201 gcaggcggtg cgcaggccgg tgaagccgcc ggggccgcgg ccgaaggcga cggcgtcgag
     1261 gtcggagaat gcccagccgg cgcgctgcag cagcgcgatg agcatcggca gcgcctgcgc
     1321 cgacgcggcg gcgccgccgt ccaggtccag cggctcgacg cggcaggccg cccgcagcaa
     1381 cgggccgccc ggcgcgcggc cgtcgtcgat ggcggcgagc gccagccgtt cggtggcgct
     1441 gtcgatggcc agcagtttca cgagcgcgtc cctgcccggc cggaaacgag cggaatcgac
     1501 gacgcgcagg cgcgttcggg tcgggaatgg cggggcagcc ggcgcatggg aaagagtgta
     1561 gccgtgcgat catggaatcg cgccggtcgc cccgggcgcg cacgacactc ctgcctcttc
     1621 ccatgtccgt ccgcctccgg ctcgccaccc tcgccttcgc ccttgccgcc atcgcggccc
     1681 cgcccaccgt ctcggccgcc ccggcctggg ccctgccacc gaccatcgtc gccgcgctgg
     1741 acaaggcgca cgtgccgccg gacgcgctgg cgctgtgggt gcagccggtg gacgcgagcg
     1801 ggccgtcctg gggctggaac cccgacttcg gcgtcaaccc ggcctccgtg ttcaagctgg
     1861 tgaccaccag cgcggccctg gacaccttcg gcccggcctg gacgtggaag accggcgtcc
     1921 acgtcaccgg cccggtccgc aacggcgtgc tcgacggctc ggtcgtcatc gtcggctccg
     1981 gcgaccccag cctcgtgatg gagcgcgtgt ggctgctgct gcgccagctg cgcgacgcgg
     2041 gcgtacgcga catccgcggc gacatcgtcc tcgaccatac cgccttcgcg ccgagcgcgc
     2101 gctcgccggc cgacttcgac aacgcgccgt cggagcccta caacgtgctg cccgacgcgc
     2161 tgctgatgaa cttccgttcg gtcacgctcg gcttccgtcc gcagcccggc ggccgcgtgg
     2221 cgcgcgtcac gtccgagccg ccgctggccg ggctggcggt ggcaccgacg gtgccgctgt
     2281 ccaaggccgc gtgcggtgac tggcgtgccg gcctgcggct gtcggccgac gacccggcgc
     2341 gctggcgctt cgcaggcagc tacccggccg cctgcggcga tcgcaactgg ccgctcgcct
     2401 acccggatcc ggccacctac gagaaccggc tcgtggccgc gctgtggcgc gagctgggcg
     2461 gcaagctcgc gggcaaggtg cacgccggca cgctgcccga gggcgccacg cacgcgttcg
     2521 acttcgagtc gccgcagctc gcgcagatcg tgcgcgacat caacaagttc agcaacaaca
     2581 cgatggccga gcagctgttc ctgacgctcg catcgaacgc gtcggccacg gcctccgtcg
     2641 cgcagcccga acaggcgcgc gcggtgctgt cgcagtggct gcacgaacgg ctgggccagg
     2701 ctgccgacgg cgtcgtcgtc gccaacggct cgggcctcgc gcgcgagacg cgcatcagcg
     2761 cacgcacgct gggcgcgctg ctgcagtcgg actggcgcag ccccgcgatg cccgagctga
     2821 tgagctcgct gccgatcgtg ggcgtcgatg gcacgctccg ccgcagcaag gccgaggcgg
     2881 gtcgcgcgca cctgaagacg ggctcgatgc gcgacgtggt ggccgtcgcc ggctacgtgc
     2941 tggccgacag cgggcgccgc tacgtgctcg tggccgtggt caacgacccg aacgccggcg
     3001 ccgcgcaccc ggtgctcgat gcggcgatcg actgggtcgc gcacgatccg gcgcaggcgc
     3061 ccaggacgcc ctgaaaggac gaatcccgcg ggcatcgctg cgcgcgggat ctgtgggtgc
     3121 cggcgccgag gcgccggcga gccgccgatg cggcctcaga acgcgtagcc gatggacgcg
     3181 ttgatcgcga ccgggttcag gcgcgccttg gtggtctggc cggtggacag ctgggccttc
     3241 gtgttgatga aggtcttgag cacggcggcg tcgacgaacc agtgcttgtc gatcttgtag
     3301 ttcaggccga cctcggtggt cgcgccccac tccgtgtcgc cgccgatggt ggtcggcggg
     3361 ccgccggggt tcgtgaccgc ggtcagggcg gccgagccct tgctgccgta gaacttggcg
     3421 aaggtggggc cggcgccgac gtagggacgc agcggcgcat cggcgggcat gaagcggtac
     3481 tgcagcagca ccgtcggcga gatctggtgg atgctgccga tcttgccgac gccctgaacg
     3541 gagcccgtgc cgatgatgtc gtgcttgtac ggcaggccgc ccagcgcctc gacggagatg
     3601 tcgtccgtga tcatgtacgt cgccgtgaag aacagcgcgc tggccgactt gatgtcgatc
     3661 gtcgagccgg gcagcgccgg ggccgacagg tcggtgctgc gcaccttggg catgatcttg
     3721 ttccagccca gcttgaccat gacctggccc gcgacctgcg cctgcgcggc accggcgccg
     3781 caaagcgcgg cggtggcaac gagggcggat gcaatggagt gcttcttcat gaatgtgtcc
     3841 ttgcgtgtgt ctcggtcgcc ggcttacagc cagcccttga tggccagttg ctcgctgacg
     3901 agctcggcca gaaggcggta gccgtacggg gtcgggtgca cgctgtcggc gtactcgtag
     3961 gtgttggcgg cggtggcggc ggcacccgag gcggccggcg cgacggccgg gatcagcgtg
     4021 gctgccgtgc agaccagcga gctgctggtg gtgcaggtgg gatgcgacac gtccgtcagg
     4081 ccgtactgcg ccatgttggt ggcctggtcc tcgctggccg agaacgcatc gacgaccacc
     4141 aggttggcgc tcgccgtcag gccggcctgc agcgcgctgt tgaaggcctg ggtcatgctc
     4201 ttgaccagcg cctggccctg cgcgccggat ccgacggcaa acggcgtcag actgacatcg
     4261 ggcaggttga ccacgacgac gtgcgtggcg ccgttggcca ggatctgcgt gttcacgtag
     4321 gccgcctctt cggtgccggc ctgcgccatc gccgtgaccg cggccgtggc ggcggccgtc
     4381 gggtcgccgc ccgcggccac ggtggcctgg aagaccgcga cgttgatgaa gatgtcgttg
     4441 ccgccggcga gcaccgtgac gagctggtcg ctggtgaact tgccgccatt gtgggcgagg
     4501 tagttggcga tctgcgtctg caccggcacc gtcagctggc cgatggcgtt gctgtacgtg
     4561 ctgggatccg cggcgttgaa cagaagcgcg ttgcctgggc cgacggggtt ggtcacgcgg
     4621 gcgccgccct gcgcgtagtt cgtgcagctg ccgcccgcgc tggcgtcgtg gaacgtggcg
     4681 ggcaccggcg ggacgccgac cacggcctgg atggtgttca gtcccgtctc ggcggcacag
     4741 ggcgcggtca ggtcgagcgt ggcggagagg aactcggtcc agttcgtcca cagcaggccg
     4801 gtggcgccga tgccgttgat cgaatagtgt ccaccgccct ggagcgcgac cgccccgacg
     4861 gcgtagctgc cgacgtcgct gaggctgtcg ccgaagctga ccatggacgt gaaggcgacg
     4921 cgcggcttct ggtcgccggc ctcgttgccg ccgcagccag ccaggaaaac cgcggccgca
     4981 agcgccgtgg tcaggacgtg gaatttcttc atgtgcacct cgccggaggg aaagtcaaaa
     5041 ggtcaatcga acggtcgttc gatttggaaa aaactatacg cacccggggt gtacgccatg
     5101 atgggggact tccctgtcga catcatgcaa acaacacacg cgtgcgcact ggacgccgac
     5161 gttatgacga ggtccgcgcg gtgtgaagac gagaaggccc tcgcgcggca tggacgtcgc
     5221 gggagggcca catggtgcag cgcagcaccg gcgcggagga ttgcgccgcg gatcagaacg
     5281 ggttggagtg ggcgcgctgc gcggcggcga cgccgatctg gttctgcatc gtcgcgccca
     5341 ggtgcgtcgc gtccgcccac aggaagtggg ccgacgcgac ctggccggtg gtcgggtc
//
LOCUS       DATCRO010000044         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_326773, whole genome shotgun sequence.
ACCESSION   DATCRO010000044 DATCRO010000000
VERSION     DATCRO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326773"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VIN75_01225"
     CDS             <1..1216
                     /locus_tag="VIN75_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972807.1"
                     /translation="YVMTADHGPISFHWDAATGALSAPVNAAPAIHGLGIAFHGASMY
                     LSAFDGTIHKLNDTNGNGVWGEPGELDVAIVTGLPQGDHDTDQMQVIGNALYVGIGRR
                     TINGHSGAWTGQVIDDFGGKGFALGGLGRTWGDSAYNGTIAWIPDLRAVVDTPASANA
                     WNTTPPTLTQALVQHDSGPFTLRGAGRLVVHSAGTRNPFGLCVDAAHALYFTNNFNRT
                     ATLGNGEAGFGLRGDKLDSDVSRDVQDQFFKASPGADYGYTDVNWRGHNPMLTPTADG
                     YHRVRSLTFDNLFNRGPYVLHDPAHPDGLGPSSSADGCAFWTATGLPATLRGNAFIAR
                     YNGKITEAPGGAGASLTYADLVAVDAATGRVRQVAAGFSHPLAVLADPAGRLLVADYG
                     SHQIYAISPVAP"
     gene            1748..>2027
                     /locus_tag="VIN75_01230"
     CDS             1748..>2027
                     /locus_tag="VIN75_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEY8972808.1"
                     /translation="MSNEAQQKRLKLLAALSAAVAASVPAAAMAANPPNCSAWNSTIA
                     YTAGAVVVDQGKTYTANWWTQGQEPVNNNGGAGSGQPWTLSSGCSTTPP"
BASE COUNT          360 a          761 c          619 g          287 t
ORIGIN      
        1 gtacgtgatg accgccgatc acggccccat cagcttccac tgggacgccg cgaccggggc
       61 gctgtccgcg cccgtcaacg cggccccggc catccacggc ctgggcatcg ccttccatgg
      121 cgcgagcatg tacctcagcg cgttcgacgg cacgatccac aagctcaacg acacgaacgg
      181 caatggcgtg tggggcgagc ccggcgagct ggacgtggcc atcgtcaccg gcctgccgca
      241 gggcgaccac gacaccgacc agatgcaggt catcggcaac gcgctgtacg tgggcatcgg
      301 ccggcgcacg atcaacggcc attccggcgc gtggacgggc caggtgatcg acgacttcgg
      361 cggcaagggc ttcgcgctcg gcggcctcgg ccgcacctgg ggcgattcgg cctacaacgg
      421 caccatcgcc tggatccccg acctgcgcgc cgtcgtcgac accccggctt cggccaacgc
      481 gtggaacacg acgccgccga ccctcacgca ggcactggtg cagcacgaca gcgggccgtt
      541 cacgctgcgc ggcgcgggcc ggctggtcgt gcactcggcg ggcacgcgca acccgttcgg
      601 cttgtgcgtc gacgccgcgc acgcgctgta cttcaccaac aacttcaacc gcacggcgac
      661 gctgggcaac ggcgaggcgg ggttcggcct gcgcggcgac aagctggaca gcgacgtcag
      721 ccgcgacgtg caggaccagt tcttcaaggc ctcgcccggc gccgactacg gctacacgga
      781 cgtcaactgg cgcggccaca acccgatgct cacgcccacc gcggacggat accaccgcgt
      841 gcgcagcctc accttcgaca acctgttcaa ccgcgggccg tacgtgctgc acgacccggc
      901 gcacccggac ggcctcggac ccagttcatc ggccgacggc tgcgcgttct ggacagccac
      961 cggcctgccc gcgacgctgc ggggcaacgc gttcatcgcc cgctacaacg gcaagatcac
     1021 cgaggccccc ggcggcgcgg gcgcatcgct gacctacgcc gacctggtgg cggtcgatgc
     1081 ggcgaccggc cgcgtgcgcc aggtcgccgc cggcttcagc catccgctgg cagtgctcgc
     1141 cgacccggcc gggcggctgc tggtggccga ctacggcagc caccagatct acgcgatctc
     1201 gccggtcgcg ccctgaccga acgcgcgcag gccggactcg caacgcgaat ccggcctttt
     1261 gtcacggccc tccccaaagt ggttaaggcg ataccctatt cccggtattg cccgctattt
     1321 gctagcgcaa cttcatactc gacgacataa ccagtatgag cactggacag caccagccgg
     1381 ccgaccgtcc agcgccagag cggacccaca gttctgccac agcgcacccg tgaaatcctc
     1441 gccttcgccc ctgtgcgccg gcggattcgg aggaatcttc agaaccggcg cacaccgccg
     1501 ggcaccctcc gcatccggca ccgcgaaagt atgtacatac tgatttatta tttgaccggc
     1561 atactggtct tgtagtggtt atccatcatg accgatttgt gaccaagcct tacgctgacc
     1621 cacgatccga acgattcatg gcgccagcgc tcccgaattc aagataacga cgcggcagcc
     1681 ttctcggatc gaatccggcg tcccctcccc gagacgctcc ctagcccgga aagcacggag
     1741 aagaagcatg tccaacgaag cccagcaaaa gagactgaag ttgctggccg ccctctcggc
     1801 ggctgtcgca gcgtcggttc ccgcggccgc catggccgcc aacccgccca actgttccgc
     1861 ctggaactcg acgatcgcct acaccgcagg cgccgtcgtc gtcgaccagg gcaagaccta
     1921 caccgccaac tggtggacgc agggccagga accggtgaac aacaacggcg gcgccggctc
     1981 cggccagccc tggacgctgt ccagcggctg ctcgaccacc ccgccgc
//
LOCUS       DATCRO010000045         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_833115, whole genome shotgun sequence.
ACCESSION   DATCRO010000045 DATCRO010000000
VERSION     DATCRO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833115"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..842)
                     /locus_tag="VIN75_01235"
     CDS             complement(3..842)
                     /locus_tag="VIN75_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8972809.1"
                     /translation="MTTPTRQLPWSLDDIPYAAIEAARIRDDDALFLLVCSASFVESG
                     SDTYTGNLVAHFADDDEVGTWLREHWEPEELQHGRALKAYVRHVWPEFDWDAAYADFF
                     AEYQRVCTIEELEPTRGQEMAARCIVEMGTTTYYQAIHAATTEPVLRGLAWRIRSDEV
                     RHYKNFHAFFQKYQRAEHLGRRHVLAALVRRAVELRNEDAAIALRHVAAWRRRTRLDD
                     GAPGEVPSDSELKHRAYGVVSRHLPMDLAVRMALKPLRLSPGVQRWIAKPLATLGAGI
                     VRP"
     gene            complement(925..1437)
                     /locus_tag="VIN75_01240"
     CDS             complement(925..1437)
                     /locus_tag="VIN75_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972810.1"
                     /translation="MRPETLFQIHLLAGYAAWLLCFGAFIRPWLKSLERDTALRAIAM
                     LHGFRFFGLVFILPGFVGSGLPSGFATPAAWGDFATGVLALLAVASFRVRPLFRLLVV
                     AFNVIGFADLLLAYAHAVQLGLPEVAGQLGAAYAIPIIYVPLLAITHVAAFGLLARPA
                     RAYAASAGAA"
     gene            1521..2501
                     /locus_tag="VIN75_01245"
     CDS             1521..2501
                     /locus_tag="VIN75_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972811.1"
                     /translation="MKFNYTVERHALDGLEAFLRVAARGSFRQAAADLAVTPSAVSQA
                     IRAIEARLGVALFTRTTRSVGLTEAGQRFLEHVRPAFDAIVAATEEAKGQSRRPSGLL
                     RLAVPPSVVPLVLQPVLASFAEAHPDVVVEIASSEELVDLARDGFDAGIRLGEFIAAD
                     MVAVRLTPPLRFAIVASPAYLARHGRPQALADLTAHACIRLRRAGGAIAPWRVEDEGR
                     AIELAVTGPVVVNDYATMLGAALGGVGLAQLPEPVAASHVAEGRLEEVLPALAPSTPG
                     VFLYHPGRLQVLPKLRAFIDHVRAHAAAPVRLAPGPAPRPGARKGRGVAG"
     gene            2530..>2862
                     /locus_tag="VIN75_01250"
     CDS             2530..>2862
                     /locus_tag="VIN75_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8972812.1"
                     /translation="MQVSSYLKVSALVAVATIALKAGAWYVTGSVSLLSDAIEGLVNV
                     VGAVFALLMVTWAAQPPDEGHPYGHHKAEYFSSGFEGVLIVAAAIAIVGAAGERLFHP
                     APVQQIGLG"
BASE COUNT          389 a         1023 c         1059 g          391 t
ORIGIN      
        1 gctcagggcc gcacgatccc cgcgccgagc gtggccagcg gcttggcgat ccagcgctgc
       61 acgcccgggc tgaggcgcag cggcttgagc gccatgcgca cggccagatc catcggcagg
      121 tgccggctca cgacgccgta ggcccggtgc ttcagctcgg aatccgacgg cacctcgccc
      181 ggcgcgccat cgtccaggcg cgtgcggcgg cgccaggccg cgacgtgccg cagcgcgatc
      241 gcggcgtcct cgttgcgcag ttcgaccgca cgccgcacca gtgcggccag cacgtggcgg
      301 cggcccaggt gttccgcgcg ctggtacttc tggaagaacg cgtggaagtt cttgtagtgg
      361 cgcacctcgt cgctgcggat gcgccacgcg agtccccgca gcaccggctc ggtcgtggcg
      421 gcgtggatcg cctggtagta ggtggtcgtg cccatctcca cgatgcagcg cgccgccatc
      481 tcctggccgc gcgtgggctc gagctcctcg atcgtgcaca cgcgttggta ctcggcgaag
      541 aagtcggcgt acgcggcgtc ccagtcgaac tcgggccaca cgtggcgcac gtaggccttc
      601 aacgcgcggc cgtgctgcag ctcctcgggc tcccagtgct cgcgcagcca ggtgccgacc
      661 tcgtcgtcgt cggcgaagtg cgcgaccagg ttgccggtgt aggtgtccga gccgctctcg
      721 acgaacgagg cgctgcacac gagcaggaac agcgcgtcgt cgtcacggat gcgcgcggcc
      781 tcgatggccg cgtaggggat gtcgtccagc gaccacggca gttgtcgggt gggggtcgtc
      841 atccgccttg cgtggcaatc ggcgtacgcg cgggccgtcc tcgacctgaa cgcggcccgg
      901 acgcgctcgc gggacggtcg ggcttcacgc agcgccggcc gacgccgcgt acgcccgcgc
      961 gggacgcgcg agcagcccga acgccgcgac gtgcgtgatc gccagcagcg gcacgtagat
     1021 gatcgggatc gcataggccg cgccgagctg gcccgcgact tcgggcaacc ccagctgcac
     1081 cgcgtgcgca taggcgagca gcaggtctgc gaagccgatg acgttgaacg ccaccacgag
     1141 cagccggaag agcggccgca cgcggaacga cgccacggcc agcagcgcca gcacgcctgt
     1201 ggcgaagtca ccccaggccg cgggcgtcgc gaagccggac ggcaggccgc tgccgacgaa
     1261 gcccggcagg atgaacacga ggccgaagaa gcggaagccg tggagcatgg cgatcgcgcg
     1321 cagcgcggtg tcgcgctcga gcgacttcag ccacggccgg atgaacgcgc cgaagcaaag
     1381 cagccacgcg gcgtagccgg cgagcaggtg gatctggaac agggtctccg ggcgcatggc
     1441 gggctccttt cgatgaggtc gtcgagacga tcgtagggac gggacattct ccgcgccagt
     1501 ctccataatc tgcatgaacc atgaagttca actacacagt cgagcgccac gccctcgacg
     1561 gcctcgaagc gttcctgcgc gtggccgccc gcgggagctt ccgccaggcc gcggccgacc
     1621 tggcggtgac gccgtcggcc gtgagccagg cgatccgggc gatcgaggcc cggctgggtg
     1681 tcgcgctgtt cacgcgcacc acgcgcagcg tcgggctgac cgaggccggc cagcgcttcc
     1741 tcgaacacgt gcggccggcg ttcgacgcga tcgtcgcggc gaccgaggaa gccaaggggc
     1801 agtcccggcg gccgtccggg ctgctgcggc tggcggtgcc gccgtcggtg gtgccgctcg
     1861 tgctgcagcc ggtgctggcc tcgttcgcgg aggcgcatcc ggacgtggtg gtggagatcg
     1921 cctccagcga ggagctcgtc gatctcgcgc gcgacggctt cgacgccggc atccgcctgg
     1981 gcgagttcat cgcggccgac atggtggccg tgcgcctgac gccgccgctg cgcttcgcga
     2041 tcgtcgccag cccggcctac ctcgcgcggc acggacgccc gcaggcgctc gccgacctca
     2101 ccgcccatgc ctgcatccgc ctgcggcgcg cgggcggcgc gatcgcgccg tggcgcgtcg
     2161 aggacgaagg ccgcgcgatc gagctcgcgg tcacggggcc ggtggtggtc aacgactacg
     2221 ccacgatgct gggcgccgcg ctcggcggcg tcggcctcgc gcagttgccc gagccggtcg
     2281 ccgcgtcgca tgtcgccgaa ggccggctgg aggaggtgct gccggcgctc gcgccgtcga
     2341 cgccgggcgt gttcctctac cacccgggcc gcctgcaggt gctgcccaag ctgcgcgcgt
     2401 tcatcgacca cgtgcgcgcg cacgcggccg cgcccgtgcg cctcgcgccc gggccggcgc
     2461 cgcgccccgg cgcgcgcaag gggcggggcg tggccggatg agtccggcgg cgcggcgaca
     2521 atacgggcca tgcaagtcag ttcgtacctc aaggtctcgg ccctggtcgc cgtcgcgacg
     2581 atcgcattga aggccggcgc ctggtacgtc acgggttcgg tgagcctgct gtccgatgcc
     2641 atcgagggcc tggtcaacgt ggtcggcgcc gtcttcgcgc tgctgatggt cacctgggcc
     2701 gcgcagccgc ccgacgaggg ccacccgtac ggccaccaca aggccgagta cttctccagc
     2761 ggcttcgagg gcgtgctgat cgtcgcggcg gccatcgcca tcgtcggcgc cgcgggcgag
     2821 cggctgttcc atccggcgcc ggtgcagcag atcgggctgg gc
//
LOCUS       DATCRO010000046         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_304258, whole genome shotgun sequence.
ACCESSION   DATCRO010000046 DATCRO010000000
VERSION     DATCRO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304258"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2384)
                     /locus_tag="VIN75_01255"
     CDS             complement(189..2384)
                     /locus_tag="VIN75_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8972813.1"
                     /translation="MAPDDLSTTPNRIARSAPIAVRLALAFGLLLALLAGVVALVLKQ
                     AELVGESSRVVAESSLRQVLLARQAEKAAQAGAQHLHTLFLLEKREERVPVYALIDTE
                     FADQKAALSQLLADAHAVEDAVVVERLISTRGRFEAAFQKTVESVELDLNVARPIMIK
                     ETLPALNDMLYALDALVAVKSAQANATISSIRMQQAKSREYVLELSGLAVVVALFCAV
                     MITRSITRPLALTVALAREIADGKLDSPMPEAGRDEVGRLITALGEMRDSLAQREARI
                     VDLAFRDPLTGLANRTLFNDRLDQAVGTATRTGHAMSVLLIDLDRFKNVNDVLGHPVG
                     DELLNQVAGRLTRELRRTTDTVARIGGDEFAVLLPAQGREGAALLARQLLTALDTPVT
                     IEGQTVDVSGSIGIATFPEDGSNPTELMAHADSAMYVAKQTSSGYAAFTQKMTRRTDH
                     GLSLLSDLRRAIDENQFYLQYQPKISLADRSCLSAEVLLRWRHPTRGFVPPDQFIPFA
                     EQTGIIKSITRWVLARGVAQLAAWRASGLEMSLNINVSTRDLVQQDLPAIVRAQLLAE
                     GVAARHLCLEVTESAIMEDPAHALASLQALHDMGVQLSIDDFGTGYSSLAYLKKLPVQ
                     ELKIDRSFVMNLDADDNDLNIVRSTIGMAHHMGLKVVAEGVETESVAAKLRMLGCDGA
                     QGYLFSRPLNADDFQAWAVAAHAANPPLRPLPPGETPSSDLFVPLPAPI"
BASE COUNT          344 a          847 c          910 g          367 t
ORIGIN      
        1 gtcctggacg agccggcgcc ccggcgaggg cgcatgggcg gcgagccggc cgtcagatcg
       61 gcgccggcag cggcacgaac aagtcggagg agggcgcctc gccgtgggcc aaaagagccc
      121 gtcatcctgc gcggagtcgc aggatccacc tcgccccgcc caggccgttc cggtcccggg
      181 gacctgtctc agatcggcgc cggcagcggc acgaacaggt ccgacgacgg cgtctcgccg
      241 ggcggcaagg ggcgcagcgg cgggttggcg gcgtgcgccg cgaccgccca ggcctggaag
      301 tcgtcggcgt tgagcggccg gctgaacagg tagccctgcg cgccgtcgca gcccagcatc
      361 cgcagcttgg cggccaccga ctccgtctcg acgccctcgg cgaccacctt caggcccatg
      421 tggtgggcca tgccgatcgt cgagcgcacg atgttcaggt cgttgtcgtc cgcgtccagg
      481 ttcatgacga acgagcggtc gatcttgagt tcctgcaccg gcagcttctt gaggtaggcg
      541 agcgacgagt agcccgtgcc gaagtcgtcg atcgacaact gcacgcccat gtcgtgcagc
      601 gcctgcagcg aggccagcgc gtgcgccgga tcctccatga tcgcgctctc ggtcacctcc
      661 aggcacaggt ggcgcgcggc cacgccctcg gccagcagct gggcacgcac gatggcgggc
      721 aggtcctgct gcacgaggtc gcgcgtggac acgttgatgt tcagtgacat ctccagcccg
      781 ctcgcgcgcc acgcggccag ctgcgccacg ccgcgggcca gcacccagcg cgtgatcgac
      841 ttgatgatgc cggtctgctc ggcgaacggg atgaactggt cgggcggcac gaagccgcgc
      901 gtcgggtggc gccagcgcag cagcacctcg gcggacaggc agctgcggtc ggccagcgag
      961 atcttgggct ggtactgcag gtagaactgg ttctcgtcga tggcgcggcg caggtccgac
     1021 agcaggctca ggccgtggtc ggtgcggcgc gtcatcttct gcgtgaaggc ggcgtagccg
     1081 gagctcgtct gcttggcgac gtacatcgcc gagtcggcgt gcgccatcag ctcggtcggg
     1141 ttgctgccgt cctcggggaa ggtggcgatg ccgatcgagc cgctgacgtc caccgtctgg
     1201 ccctcgatcg tcaccggcgt gtccagcgcc gtcagcagct ggcgcgccag cagcgccgcg
     1261 ccctcgcggc cctgcgcggg cagcagcacg gcgaactcgt cgccgccgat gcgcgcgacg
     1321 gtgtcggtgg tgcggcgcag ctcgcgcgtg aggcggccgg ccacctggtt cagcagctcg
     1381 tcgccgacgg ggtggcccag cacgtcgttg acgttcttga agcggtccag gtcgatcagc
     1441 agcaccgaca tcgcatgccc cgtgcgcgtg gcggtgccca cggcctggtc gaggcggtcg
     1501 ttgaacagcg tgcggttggc caggcccgtg agcgggtcgc ggaacgcgag gtcgacgatg
     1561 cgcgcctcgc gctgcgccag gctgtcgcgc atctcgccga gcgcggtgat caggcggccc
     1621 acctcgtcgc gcccggcctc gggcatcggc gaatcgagct tgccgtcggc gatctcgcgc
     1681 gccagcgcca cggtcagcgc cagcggccgc gtgatcgagc gcgtgatcat caccgcgcag
     1741 aacagcgcga cgacgacggc caggcccgac agctcgagca cgtactcgcg gctcttggcc
     1801 tgctgcatgc ggatgctgga gatcgtcgcg ttggcctgcg ccgacttcac ggccaccagc
     1861 gcgtccagcg cgtagagcat gtcgttgagc gccggcagcg tctccttgat catgatcggc
     1921 cgcgcgacgt tgaggtccag ctcgaccgat tccaccgtct tctggaacgc ggcctcgaag
     1981 cggccgcgcg tggagatcag gcgctcgacg acgacggcgt cctcgaccgc gtgggcatcg
     2041 gccagcagtt gcgacagcgc ggccttctgg tcggcgaact ccgtgtcgat cagcgcgtag
     2101 accggtaccc gttcctcgcg cttctccagc aggaacagcg tgtgcaggtg ctgggcgccg
     2161 gcctgggcgg ccttctcggc ctggcgcgcg agcagcacct ggcgcaggct ggattcggcg
     2221 acgacgcggc tggactcgcc caccagctcg gcctgcttga gcaccagcgc caccaccccg
     2281 gccagcagcg ccagcagcag gccgaacgcg agcgccagcc gcaccgcgat gggggcgctg
     2341 cgggcgatcc ggttcggcgt ggtcgacagg tcgtcggggg ccatggtggg ggtcggcaat
     2401 cggcttcggg caggggaagt ctggtggtta tcgacgggtt tgccggggaa attgaggccg
     2461 tggcgggg
//
LOCUS       DATCRO010000047         5907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_113005, whole genome shotgun sequence.
ACCESSION   DATCRO010000047 DATCRO010000000
VERSION     DATCRO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113005"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VIN75_01260"
     CDS             complement(<1..631)
                     /locus_tag="VIN75_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972814.1"
                     /translation="MKSSLSILASATLLATLAACGGGGGDSPSQPGGDKVTITSANQN
                     QVVRAGVNAGMTVSMAQNRAGGASPAGVADRSHALGTVMQRAMSAAQASRKGIASATA
                     HPSVSVSDTEPCGVSGTLTVSVDDRDGNNALSSGDVISVSFAQCKDTASSSINGAIAI
                     SLTGTPTATQFTASANFQNVAVVEGGVTSTIAGSVTVAENDTDLDTVTTL"
     gene            complement(789..1616)
                     /locus_tag="VIN75_01265"
     CDS             complement(789..1616)
                     /locus_tag="VIN75_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8972815.1"
                     /translation="MWRVIGPSRRNLRAWQLVLLALLFVFWHVMTTPGLVPPFLFSDD
                     NQAAFFFGQPLKIFDRIGHWFFVDADIYKHLVVTLWETVLAFGIGTVLGLGMGLWLAL
                     SPLAAALLDPYIKALNAMPRVILAPIFAVWFGLGVASKVALGVTLVFFIVFFNVYQGV
                     KEVSPVILANARMLGAGRRQLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSA
                     QGVGYLILQAEGSFDIDTVMAGILVLTAFALVLDALVGRVEKALMKWQPQAAESEKV"
     gene            complement(1622..2437)
                     /locus_tag="VIN75_01270"
     CDS             complement(1622..2437)
                     /locus_tag="VIN75_01270"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8972816.1"
                     /translation="MSSPALSLEGVSCTFASRDGGTRGFFTAVRDVTLAVAAGEFVSV
                     VGPTGCGKSTLLNMAAGLLAPGMGAVRVFGEPLAGLNRRAGYMFQAESLMPWRTALGN
                     VMAGLSFRGASEADARALATQWLQRVGLAAFGDRYPHQLSGGMRKRVSLAQTLVLDPD
                     IILMDEPFSALDIQTRQLMENEVLELWGAKKKAVLFITHDLDEAIAMSDRVVILSAGP
                     GSRVVGEFRIDLARPHDVAEVRADARFVELHKAIWGVLREEVLAGYQQQLAKA"
     gene            complement(2434..3453)
                     /locus_tag="VIN75_01275"
     CDS             complement(2434..3453)
                     /locus_tag="VIN75_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8972817.1"
                     /translation="MQRRSVVLLAAGALAAPALLRAQPLEKPKLTIAVGGKNLLYYLP
                     LSIAESQGYFKAEGLDVTIVDFAGGSKALQAVVGGSADVVSGAFEHTLLMQPKGIKLR
                     AFALQGRAPQIVLGINPKTMPGYKSVADLKGRKIGVTAPGSSTNVLANFVLAKAGLKP
                     GDVSIIGVGAGAGAVAAMRAGQIDAISNLDPVITLLQRSGDLKIVSDTRIVAEADKAF
                     GGPMPAGCMYAPQAFVDKNPATVQAIADAIVRADKWIQGAGPADIVKAVPESYLLGDR
                     AVYIDAFLASKGALSPDGLFPAEGAATALRALQSVDESMKTARIDLAAAWTNDFVKKA
                     NARKA"
     gene            complement(3602..5488)
                     /gene="dxs"
                     /locus_tag="VIN75_01280"
     CDS             complement(3602..5488)
                     /gene="dxs"
                     /locus_tag="VIN75_01280"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8972818.1"
                     /translation="MSNNLLATIASPADLRRLSRSELRALCDELRQYILHSVSKTGGH
                     LSSNLGTVELTVALHYVFNTPYDRLVWDVGHQTYPHKVLTGRREQMSTLRMYGGLAGF
                     PKRDESEYDAFGTAHSSTSISAVLGMAHAAKLKGENRKAVAIIGDGAMTGGMAFEAMN
                     NAGVSMADMLVILNDNDMSISPPVGALNRYFARLMSGKFYARAREGAKSMLKNTPLYE
                     FARRFEEHTKGMVVPGTIFEEFGLNYVGPIDGHDLDSLIPTLENLRDAKGPQFLHVVT
                     KKGMGYKLAEADPVNYHGPGKFDVAVGLQKPATPAKQTFADVFGSWLCDMAAADKRLI
                     GITPAMCGGSGMTEFERRFPERFHDVGIAEQHAVTFAAGLACEGLKPVVAIYSTFLQR
                     GYDQLIHDVALQNLPVVFALDRAGIVGADGPTHCGAYDIAFIRCVPGMSLLTPADERE
                     TRRALSTAFAQEHPVGVRYPRGAGAGVKPEADLDVLEWGKAERRREGRRIALLAFGPL
                     LYPALEAGEKLDATVVNMRFVKPLDVEMVLELARTHEALVTLEEGSIMGGAGSAVLEA
                     LAAAGLQVPVLQLGLPDRFVEHGDPVRLMHECGLDAEGIEQSVRRRFGPSLAVVRSTT
                     VANG"
     gene            complement(5550..>5907)
                     /locus_tag="VIN75_01285"
     CDS             complement(5550..>5907)
                     /locus_tag="VIN75_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8972819.1"
                     /translation="RRKTGVLLQASVMMGAACGPADATALAALSEYGAAIGLAFQVVD
                     DVLDVTQESHVLGKTAGKDVDQNKPTFVSMLGLEAAQKYAHELRDQAHAALARSGLGQ
                     AAYLACLADKIVERDN"
BASE COUNT          872 a         2060 c         2072 g          903 t
ORIGIN      
        1 tcagcgtggt caccgtgtcg aggtcggtgt cgttctcggc gaccgtgacg ctgccggcga
       61 tcgtcgacgt cacgccgccc tcgaccaccg cgacgttctg gaagttggcg ctggcggtga
      121 attgcgtggc ggtgggcgtg ccggtgagcg aaatggcgat cgccccgttg atcgaggagc
      181 tcgccgtgtc cttgcactgc gcgaagctca ccgagatgac gtcgccgctg ctgagcgcgt
      241 tgttgccgtc gcggtcgtcg accgagacgg tgagcgtgcc gctgacgccg cagggctcgg
      301 tgtcgctgac cgagaccgac gggtgcgccg tggcgctcgc gatgcccttg cgcgacgcct
      361 gggccgcgga catcgcacgc tgcatcacgg tgccgagcgc gtgcgagcga tccgcgacgc
      421 ctgcgggcga cgcgccgccg gcccggttct gcgccatcga caccgtcatg cccgcgttga
      481 cgcccgcgcg cacgacctgg ttctggttcg cggacgtgat cgtgaccttg tcgccacccg
      541 gctgcgacgg cgaatcgccg cccccgccgc cgcaggcggc cagggtcgcc aacagcgtgg
      601 cggaggcaag gatggaaaga ctggacttca tgaagaggca ctccctgtat cggattcgtt
      661 ggatcgccgg ctgcaccggt cgcagccgag acactaacac cgacgcaccg cccttcggcg
      721 ccgcgccgcg gagcgaacgt gaagtcgtcg atgcaggcac cgccggtatc gcgtggtgcg
      781 cccagcccct agaccttctc gctctccgcg gcctgcggct gccacttcat caatgccttc
      841 tcgacgcgcc cgaccagcgc gtccagcacc agcgcgaacg cggtcagcac gaggatgccg
      901 gccatcacgg tgtcgatgtc gaagctgccc tcggcctgca ggatcaggta gcccacgcct
      961 tgcgccgagc ccaggtactc gcccaccacc gccccgacga acgcgaggcc gaccgaggtg
     1021 tgcagcgagc tgaacaccca gctggtggcg ctgggcaggt agacgtggcg cagcagctgc
     1081 cgccggcccg cgcccagcat gcgcgcattg gccaggatca ccgggctcac ctccttgacg
     1141 ccctggtaga cgttgaagaa cacgatgaag aagaccaagg tgactccgag cgccaccttg
     1201 ctcgcgacgc cgagcccgaa ccacaccgcg aagatcggcg ccaggatcac gcgcggcatc
     1261 gcgttcagcg ccttgatgta cgggtcgagc agcgccgccg ccagcgggct cagcgcgagc
     1321 cacagcccca tgcccaggcc caggaccgtg ccgatgccga acgccagcac cgtctcccac
     1381 agcgtgacga ccaggtgctt gtagatgtcg gcgtcgacga agaaccagtg gccgatgcga
     1441 tcgaagatct tcagcggctg gccgaagaag aacgcggcct ggttgtcgtc ggagaacagg
     1501 aagggcggca cgagccccgg cgtggtcatc acgtgccaga acacgaacag cagcgccaag
     1561 agcacgagct gccacgcccg caggttgcgg cggctgggcc cgatgacacg ccacatcgcc
     1621 atcacgcctt ggccagttgc tgctggtagc cggccagcac ctcttcgcgc agcacgcccc
     1681 agatcgcctt gtgcagctcg acgaagcgcg cgtcggcgcg cacctcggcg acgtcgtgcg
     1741 ggcgcgcgag gtcgatgcgg aactcgccga cgacgcgcga gcccggcccc gcggacagga
     1801 tgaccacgcg atcgctcatc gcgatggcct cgtcgaggtc gtgcgtgatg aacagcaccg
     1861 cctttttctt cgccccccag agctccagga cttcgttctc catcagctgc cgcgtctgga
     1921 tgtccagcgc cgagaacggt tcgtccatca ggatgatgtc ggggtcgagc accagcgtct
     1981 gcgcgaggct cacgcgcttg cgcatgccgc ccgagagctg gtgcggatag cggtcgccga
     2041 acgcggccag gccgacgcgc tgcagccatt gcgtggccag ggcgcgcgcg tcggcctcgc
     2101 tcgcgccgcg gaacgacagg cccgccatca cgttgccaag cgccgtgcgc cagggcatca
     2161 ggctctcggc ctggaacatg tagcccgcgc gccggttcag tcccgcgagc ggctcgccga
     2221 acacgcgcac cgcgcccatg cccggcgcga gcaggccggc ggccatgttc agcagcgtgc
     2281 tcttgccgca gccggtgggg ccgacgaccg agacgaactc gcccgccgcc acggccagcg
     2341 tcacgtcgcg caccgccgtg aagaagccgc gcgtgccgcc gtcgcgagac gcgaacgtgc
     2401 agctgacgcc ttccaggctg agcgccggcg agctcatgcc ttccgggcgt tcgccttctt
     2461 gacgaagtcg ttcgtccacg ccgcggcgag gtcgatgcgc gcggtcttca tcgattcgtc
     2521 gacgctctgg agcgcgcgca gcgcggtggc cgcgccctcg gccgggaaca ggccgtcggg
     2581 cgacagcgcg cccttgctgg cgaggaaggc gtcgatgtag acggcgcggt cgcccagcag
     2641 gtagctctcg ggcacggcct tgacgatgtc ggccgggccg gcgccctgga tccacttgtc
     2701 ggcgcgcacg atggcatcgg cgatcgcctg cacggtggcc gggttcttgt cgacgaaagc
     2761 ctgcggcgcg tacatgcacc ccgcgggcat cgggccgccg aaggccttgt cggcctcggc
     2821 cacgatgcgc gtgtcggaga cgatcttcag gtcgccgctg cgttgcagca gcgtgatcac
     2881 cgggtcgagg ttgctgatcg cgtcgatctg gccggcgcgc atcgcggcca ccgcgccggc
     2941 gcccgcgccc acgccgatga tggacacgtc gccgggcttg agccccgcct tggccagcac
     3001 gaagttggcc agcacgttgg tggacgagcc cggcgccgtc acgccgatct tgcggccctt
     3061 caggtcggcc accgacttgt agcccggcat cgtcttggga ttgatgccca gcacgatctg
     3121 cggtgcgcgc ccctgcagcg cgaacgcccg cagcttgatg cccttgggct gcatcagcag
     3181 cgtgtgctcg aaggcgcccg acaccacgtc cgcgctgccg cccaccacgg cctgcagcgc
     3241 cttggagccg ccggcgaaat cgacgatggt gacatccagc ccttcggcct tgaagtagcc
     3301 ctggctctcc gcgatggaca gcggcaggta gtacagcagg ttcttgccgc cgacggcgat
     3361 cgtcagcttg ggcttttcca gcggctgcgc gcgcagcagc gcgggcgcgg cgagcgcacc
     3421 ggcggcgagg aggacaacgg aacgtcgttg catgaagcgg gtctccgtca aatcgttcga
     3481 cccattgtgg cgatgcatga cgtcgctgcg cagcgggcaa gcccgaggcc ccgcggtgca
     3541 gtcgggttcg cgcgctcgcg gggacgtgcg cgtgacgcga gcgcgcccgc gcgggggtac
     3601 gtcagccgtt ggcgacggtg gtggaacgca cgacggccag cgacgggccg aaacggcgcc
     3661 gcaccgactg ctcgatgcct tcggcgtcca ggccgcattc gtgcatcagg cgtaccgggt
     3721 cgccatgctc gacgaagcga tcgggcaggc ccagttggag caccggcacc tgcaggccgg
     3781 cggcggccag cgcctccagc accgcgctgc ccgcgccgcc catgatgctg ccctcttcga
     3841 gcgtcaccag cgcctcgtgc gtgcgcgcga gttcgagcac catctcgacg tccagcggct
     3901 tgacgaagcg catgttgacg acggtggcgt cgagcttctc gcccgcttcg agcgcggggt
     3961 acagcaacgg gccgaaggcc agcagcgcga tgcggcggcc ctcgcggcgc ctctcggcct
     4021 tgccccattc gaggacgtcg aggtcggcct cgggcttgac gccggcgccg gcaccgcgcg
     4081 ggtagcgcac gcccaccgga tgctcctgcg cgaacgcggt gctgagcgcg cggcgcgtct
     4141 cgcgctcgtc ggccggcgtg agcaggctca tgccgggcac gcagcggatg aaggcgatgt
     4201 cgtaggcgcc gcagtgcgtc gggccgtcgg cgccgacgat gccggcgcgg tcgagcgcga
     4261 acaccaccgg caggttctgc agcgccacgt cgtggatcag ctggtcgtag ccgcgctgca
     4321 ggaacgtgga gtagatcgcc acgaccggct tcaggccctc gcaggccagg ccggcggcga
     4381 aggtgacggc gtgctgctcg gcgatgccca cgtcgtggaa gcgctcgggg aagcgccgct
     4441 cgaactcggt catgcccgag ccgccgcaca tcgccggcgt gatgccgatc aggcgcttgt
     4501 cggcggcggc catgtcgcac agccagctgc cgaacacgtc ggcgaacgtc tgcttggcgg
     4561 gcgtggcggg cttctgcagg ccgacggcga cgtcgaactt gccggggccg tggtagttca
     4621 ccgggtcggc ctcggccagc ttgtagccca tgcccttctt ggtgaccacg tgcaggaact
     4681 gcgggccctt ggcgtcgcgc aggttctcca gcgtcgggat cagcgagtcg agatcgtggc
     4741 cgtcgatcgg gccgacgtag ttcaggccga actcctcgaa gatcgtgccg ggcacgacca
     4801 tgcccttggt gtgttcctcg aagcggcgcg cgaactcgta cagcggcgtg ttcttgagca
     4861 tcgacttggc gccctcgcgg gcccgcgcat agaacttgcc gctcatcagg cgcgcgaagt
     4921 agcggttcag ggcgcccacc ggcgggctga tcgacatgtc gttgtcgttg aggatgacca
     4981 gcatgtcggc catcgacacg cccgcgttgt tcatcgcctc gaaggccatg ccaccggtca
     5041 tcgcgccgtc gccgatgatg gccacggcct tgcggttctc gcccttgagc ttggccgcat
     5101 gggccatgcc cagcacggcc gagatggacg tcgacgaatg cgcggtgccg aacgcgtcgt
     5161 actcgctctc gtcgcgcttg ggaaagcccg cgaggccgcc gtacatgcgc agggtgctca
     5221 tctgctcgcg gcggcccgtg agcaccttgt gcgggtaggt ctggtggccc acgtcccaca
     5281 cgaggcggtc gtacggcgtg ttgaagacgt agtgcagcgc caccgtgagc tccacggtgc
     5341 ccaggttgct cgagaggtgc ccgcccgtct tgctcacgct gtgcaggatg tactgccgca
     5401 gctcgtcgca cagggcgcgc aactcggacc gcgacaggcg tcgcaggtcg gcggggctgg
     5461 caatggtggc gaggaggttg ttgctcatgt ggcgttcgag gtcgggagca cgccgcgctc
     5521 cgcaggcgtc tgcggggcgc ggtcgtgcat caattgtcgc gctcgacgat cttgtccgcg
     5581 aggcaggcga ggtaggcagc ctgccctaat ccgctgcgcg ccagcgccgc gtgcgcctgg
     5641 tcgcggagtt cgtgcgcata cttctgcgcg gcctccaggc cgagcatgga cacgaacgtg
     5701 ggcttgttct ggtcgacatc cttgccggcc gtcttcccga gcacgtggga ttcctgggtg
     5761 acgtccagca cgtcgtcgac gacctggaac gcgaggccga tggccgcgcc gtattcgctc
     5821 agcgcggcca gcgccgtggc atcggccggc ccgcaggcgg cgcccatcat gacgctggcc
     5881 tgcaacagca cgccggtctt gcggcgg
//
LOCUS       DATCRO010000048         6880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_619398, whole genome shotgun sequence.
ACCESSION   DATCRO010000048 DATCRO010000000
VERSION     DATCRO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619398"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1140)
                     /gene="cysK"
                     /locus_tag="VIN75_01290"
     CDS             complement(55..1140)
                     /gene="cysK"
                     /locus_tag="VIN75_01290"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY8972820.1"
                     /translation="MTSNPSRTTEGRGRLYDSVVDTIGDTPCIRLNRIAPQHVRLYVK
                     AEFFNPAASVKDRLAISIIEEAERRGELKPGQTIVEATSGNTGIGLAMVCAAKGYPLV
                     VTMADSFSVERRRLMRFLGARVVLTPRAAKGFGMYQKARELAEANGWFLARQFETPDN
                     ARIHENTTARELLADFDGERLDWWISGYGTGGTVTGVARVLRKERPETRIVLSEPEIA
                     QLIGSGAAQARGADNAPAASHPAFTPHPIQGWTPDFIPYVLQEAIDRRYYDEVIPVSG
                     PDAIAWSRRLAAEEGIFAGISAGATLAVAIKVAERAPAGSVLCCMLPDTGERYLSTPL
                     FEGVASEMTAEEEALSRSTPGYQMPAA"
     gene            complement(1236..1628)
                     /locus_tag="VIN75_01295"
     CDS             complement(1236..1628)
                     /locus_tag="VIN75_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8972821.1"
                     /translation="MPSDTAAPERTYQSINFADKLGKIHDHWKPRVMAEMNDYQFKIV
                     KILGDFLWHDHPDTDETFIVLDGVLRIDMRDGHVLVRAGEMFVVPRGIEHKPYAEREV
                     KMMLIEPRGVVNTGTVDSERRAENDVWI"
     gene            complement(1648..3615)
                     /locus_tag="VIN75_01300"
     CDS             complement(1648..3615)
                     /locus_tag="VIN75_01300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8972822.1"
                     /translation="MRARGAMRAAGAAIALALALAAAAAPAADLAQAIDRLVVRGFED
                     PQGANEGLRALQASTPTTPDNTRALLVGFGMVAADNHLAKETAEVAKALRELAGTVGP
                     VAEADAHLVNADLEFEGMQEENGNVEARAAVAAYSPYCETRDPALAAHCDRFNWFYAL
                     LFAAYGAQGERNPAAAAIYMHSALDAAQQSGNRALEIKATAILASLAQGDNDAELAER
                     LLGRAQALAREEDDPALRAYVQSFASDVLAEREQYEASRDAIVTAIRISRDAGLKRRE
                     AEYELSLSGAELRLEHPAEALAALARAQAFLAAHNEPGLQRQRLHDQAIALLALGRTA
                     EAKDKLRETLARYDRETGPNARIGVLRDLGPALARAGDRAGALELYTREQQLLQARND
                     GRYERDMQQVQRLIHDEQDRLQARRNAQWGAAAVACVMLALAIAYIARRQMQRNQLLT
                     SRNRVLRIQAEHDPLTGLPNRAHLRRRLGLRAARFEGALFLIDVDHFKDINDRHGHAA
                     GDAVLVAIARRLEGVLRGRDLVARWGGEEFLVMVDAMPAAEAVALAHRLMKSFTASPV
                     VVEGQPIAVTASIGFAMFPLEGKAAVTFDGAFALVDAAMYHSKAKGRACATRIASVDP
                     QLPLEQAALATGLADRAILEVHRPAEELATA"
     gene            complement(3830..4888)
                     /gene="selD"
                     /locus_tag="VIN75_01305"
     CDS             complement(3830..4888)
                     /gene="selD"
                     /locus_tag="VIN75_01305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8972823.1"
                     /translation="MTVAAPAPLRLTSFSHGGGCGCKIAPGVLSDILRTSAPGLIPPQ
                     LMVGIETSDDAAVWKLNDSQALIATTDFFMPIVDDPYDFGRIAATNAISDVYAMGGTP
                     IMALALVAMPIAQLPVEVIGRVLAGGQAVCADAGIPIAGGHTIDSVEPIYGLVVMGLV
                     HPDRVRRNADARDGDVLVLGKPLGVGVLSAALKKGRLDDAGYARLISTTTRLNKPGSR
                     LAEIDGVHAITDVTGFGLAGHALEMARGAGLRAQIDWKAVPVIEGVEALARDGFVTGA
                     SGRNWAGYGVDVRLDAALPSVARDLATDPQTSGGLLVSCRPDTVDAVLACFRDGGFPE
                     AAVVGRMVAGSPGLSITA"
     gene            4984..5889
                     /locus_tag="VIN75_01310"
     CDS             4984..5889
                     /locus_tag="VIN75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8972824.1"
                     /translation="MNLQTASSFARRRRAIVQSLAALSLGAAFLAGAPAAHAEGGVLR
                     VSAIPDEAPSELQRKFKPLGEYLKARTGMDVQFTPVTDYAAVVEGLAAKKIDLAWLGG
                     FTFVQAKLRTDGQVVPIVQRAEDEKFTSKFIVPLDSPIKSVADIKGHTFVFGAPSSTS
                     GSLMPRYFLQQQGINPERDFKTVAFSGAHDATVAFVASGRAEAGVLNSSVMDKLVESK
                     NPNAQKVRVLATTPTFFDYNWTVRPGMDPALQKKLTDAFLALDPARPADKEILDLQRA
                     SRFVPTKASNYDGIEAAGKSAGLIK"
     gene            5904..6710
                     /locus_tag="VIN75_01315"
     CDS             5904..6710
                     /locus_tag="VIN75_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8972825.1"
                     /translation="MSLRLERVGVVHANGRRALAAVDLAVAAGERVALIGPSGAGKTT
                     LLRVAAASLRPTDGHASVLEAEPWTLTARALKALRSRVGFVHQAPPIPPRQRVVTAVL
                     AGRLGQWSVGRALRSWVAPADAAGARDALARVGLAERVFERCDQLSGGQLQRVAVART
                     LYQSPRLLLADEPVSALDAATADTVLRELVAYSESAGATFIASLHAVDLALRWFPRIV
                     GVRDGAVMFDLPAAQVTPGRLAALYAGEVAAERERPVQSLPHVTLPLGSA"
BASE COUNT          860 a         2465 c         2541 g         1014 t
ORIGIN      
        1 gcactgagcg gcgccggaga cgaccagggc cgcgcgatgc ggcccctttt catttcacgc
       61 ggccggcatc tggtagcccg gcgtggagcg cgacagcgcc tcctcttccg ccgtcatctc
      121 gctcgcgacg ccctcgaaca gcggcgtcga caggtagcgc tcgccggtgt cgggcagcat
      181 gcagcagagg acggatccgg ccggggcgcg ctcggccacc ttgatggcca ccgccagcgt
      241 ggcgccggcg gagatgcccg cgaagatgcc ttcctcggcg gccaggcggc gcgaccacgc
      301 gatcgcgtcc gggccgctga cggggatcac ctcgtcgtag tagcgcctgt cgatcgcctc
      361 ctgcaggacg tagggaatga agtcgggcgt ccagccctgg atcgggtgcg gcgtgaaggc
      421 cggatggctc gcggcggggg cgttgtccgc ccctcgggcc tgcgcagccc cgctgccgat
      481 caactgggcg atctcgggct cgctgagcac gatccgcgtc tcgggccgct ccttgcgcag
      541 cacccgcgcg acgcccgtga cggtgccgcc ggtgccgtag ccgctgatcc accagtccag
      601 ccgctcgccg tcgaagtcgg ccagcagctc gcgggcggtc gtgttctcgt ggatgcgggc
      661 gttgtcgggc gtctcgaact gccgcgcaag gaaccagccg ttggcctcgg ccagctccct
      721 ggccttctgg tacatgccga agcccttggc cgcgcgcggc gtgaggacga cgcgggcgcc
      781 caggaagcgc atcaggcgcc ggcgctcgac ggagaacgag tcggccatgg tgaccaccag
      841 cgggtagccc ttggccgcgc agaccatggc cagcccgatg cccgtgttgc cgctcgtggc
      901 ctcgacgatc gtctgcccgg gcttgagttc gccgcgccgc tcggcctcct cgatgatgct
      961 gatggccagg cgatccttga ccgaggccgc cgggttgaag aactcggcct tgacgtacag
     1021 ccgcacgtgc tggggcgcga tccggttcag gcggatgcag ggcgtgtcgc cgatggtgtc
     1081 gaccacgctg tcgtacaggc gaccgcgtcc ttcggtcgtg cgggatgggt tcgatgtcat
     1141 gggctgctcc ggcgcagtgg tgcccggcga cgtgccgggt gcggcccagc ctatcgggcc
     1201 gcccgcggat gtcaatcctg cgaccgtgtc gcggctcaga tccagacgtc gttctcggcg
     1261 cgccgctcgg agtcgacggt gcccgtgttg accacaccgc gcggctcgat cagcatcatc
     1321 ttgacctcgc gttcggcata cggcttgtgc tcgatgccgc gcggcaccac gaacatctcg
     1381 ccggcgcgca ccagcacgtg gccgtcgcgc atgtcgatgc gcagcacccc gtccaggacg
     1441 atgaaggtct cgtcggtgtc ggggtggtcg tgccagagga agtcgcccag gatcttgacg
     1501 atcttgaact ggtagtcgtt catctccgcc atcacgcgcg gcttccagtg gtcgtggatc
     1561 ttgccgagct tgtcggcgaa gttgatggac tggtacgtgc gctcgggcgc ggcggtgtcg
     1621 ctgggcatgg gttctccgcg gtttcgctca ggccgtcgcg agttcctcgg ccggccggtg
     1681 gacctcaagt atggcgcgat cggccaaccc ggtcgccagc gcggcctgct ccagcggcag
     1741 ttgcgggtcg acggacgcga tgcgcgtggc gcaggcgcgg cccttggcct tggagtggta
     1801 catcgcggcg tccaccagcg cgaacgcgcc gtcgaaggtg acggccgcct tgccctccag
     1861 cgggaacatg gcaaagccga tcgaggccgt gacggcgatc ggctggccct cgacgacgac
     1921 ggggctggcc gtgaacgact tcatcagccg gtgcgcgagc gccacggcct cggcggccgg
     1981 catcgcgtcg accatcacca ggaactcctc gccgccccag cgcgcgacca ggtcgcggcc
     2041 gcgcagcacg ccctcgagcc ggcgcgcgat cgccaccagc accgcgtcgc cggcggcgtg
     2101 gccgtggcgg tcgttgatgt ccttgaagtg gtcgacgtcg atcaggaaca gcgcgccctc
     2161 gaagcgcgcc gcgcgcagcc cgagacgcct gcgcaggtgg gcgcggttgg gcaggcccgt
     2221 cagcggatcg tgctcggcct ggatgcgcag cacgcggttg cggctggtga gcagctggtt
     2281 gcgctgcatc tggcgccgcg cgatgtaggc gatcgccagg gcgagcatga cgcaggcgac
     2341 ggcggcggcg ccccactgcg cgttgcggcg cgcctgcagg cgatcctgct cgtcgtggat
     2401 gagccgctgc acctgctgca tgtcgcgctc gtagcggccg tcgttgcggg cctgcagcag
     2461 ctgctgctcg cgcgtgtaga gctcgagcgc ccccgcgcgg tcgccggcgc gcgcgagcgc
     2521 ggggccgagg tcgcgcagga cgccgatgcg cgcgttggga cccgtctcgc ggtcatagcg
     2581 cgccagcgtc tcgcgcagct tgtccttggc ctcggcggtg cggcccagcg ccagcagggc
     2641 gatcgcctgg tcgtgcaggc gctggcgctg cagtccgggc tcgttgtggg cggccaggaa
     2701 ggcctgggcg cgcgccaggg cggccagcgc ctcggcggga tgctccaggc gcagttccgc
     2761 gccgctgagg ctcagctcgt actcggcctc gcgccgcttc aggccggcgt cgcgcgagat
     2821 gcggatcgcg gtcacgatcg catcgcggct ggcctcgtac tgctcgcgct cggccagcac
     2881 gtcgctggcg aacgactgca cgtaggcgcg cagcgccgga tcgtcctcct cgcgggcgag
     2941 cgcctgggcg cgcccgagca ggcgctcggc gagctcggcg tcgttgtccc cctgcgccag
     3001 cgatgcgagg atggcggtgg ccttgatctc gagcgcgcgg ttgcccgact gctgcgcggc
     3061 gtcgagcgcc gagtgcatgt agatggccgc ggccgcgggg ttgcgctcgc cctgggcgcc
     3121 gtaggccgcg aacagcagcg cgtagaacca gttgaagcgg tcgcagtgcg cggccagcgc
     3181 cgggtcgcgc gtctcgcagt acggcgagta ggccgccacc gcggcacgcg cctcgacgtt
     3241 gccgttctcc tcctgcatgc cctcgaactc gaggtccgcg ttgacgaggt gcgcgtcggc
     3301 ctcggcgacg gggccgaccg tgcccgcgag ctcgcgcaag gccttggcga cctcggccgt
     3361 ctccttggcc agatggttgt cggcggcgac catgccgaag cccaccagca gggcgcgcgt
     3421 gttgtcgggc gtcgtgggcg tgctggcctg cagggcgcgc aggccttcgt tggcgccctg
     3481 cggatcctcg aagccgcgca cgaccaggcg gtcgatggcc tgcgcgagat cggcggccgg
     3541 cgcagcggcg gcggcgagcg cgagcgcgag cgcgattgcg gcgccggctg cgcgcatcgc
     3601 gccgcgggcg cgcacgggcg ggcgggcgcc gcgtgcgggg cggcggcggg aactatccct
     3661 ggacatggtt ctcgtggcgt gcggaaacgg cgggccgggg cgcggcggcc tcgacacgat
     3721 cccttcggca cgcgagcccc tcgattccag cgccgcggcg gtggtttcgc acggtccgtc
     3781 cccctttccg tgccaatgct ggcacagcgg ggcggcgaat gcacgcgcgt caggcggtga
     3841 tcgagagccc cggcgacccg gccaccatgc gcccgacgac cgccgcctcg ggaaagcccc
     3901 cgtcgcggaa gcacgcgagc accgcgtcga cggtgtcggg ccggcacgac acgagcaggc
     3961 cgccggaggt ctgcgggtcg gtcgccaggt cgcgcgccac ggacggcagc gcggcgtcca
     4021 ggcgcacgtc gacgccatag cccgcccagt tgcggcccga ggcccccgtg acgaagccgt
     4081 cgcgcgcgag cgcctcgacg ccctcgatga cgggcacggc cttccagtcg atctgcgcgc
     4141 gcagcccggc gccgcgcgcc atctccagcg cgtgtcccgc caggccgaag ccggtgacgt
     4201 cggtgatggc gtgcacgcca tcgatctcgg ccaggcggct gcccggcttg ttcaggcgcg
     4261 tcgtcgtcga gatcaggcgc gcgtagccgg cgtcgtcgag ccggcccttc ttcagcgcgg
     4321 ccgacagcac gcccacgccc agcggcttgc ccagcaccag cacgtcgccg tcgcgcgcgt
     4381 cggcgttgcg ccgcacgcgg tcggggtgca cgaggcccat cacgacgagg ccgtagatcg
     4441 gctcgaccga gtcgatggtg tggccgcccg cgatcgggat gcccgcgtcg gcgcacaccg
     4501 cctggccgcc ggccagcacg cggccgatga cctcgacggg cagttgcgcg atcggcatcg
     4561 ccaccagcgc cagcgccatg atcggcgtgc cgcccatcgc gtagacatcg ctgatcgcgt
     4621 tggtggcggc gatgcgcccg aagtcgtacg ggtcgtcgac gatgggcatg aagaagtcgg
     4681 tcgtcgcgat cagcgcctgc gaatcgttga gcttccacac cgcggcgtcg tcggaggtct
     4741 cgatgcccac catcagctgc ggcgggatga ggcccggggc gctggtccgg aggatgtcgg
     4801 acagcacgcc cggcgcgatc ttgcagccgc agcctccgcc gtgggagaag ctggtgaggc
     4861 ggagcggggc gggcgcggcg acggtcatgg ggcggttctc gggttttttc cgttccagcg
     4921 ggcagaatgg cgggttgcac cggcggcgca tcgcggccgg caccacattc tccccgcatc
     4981 gccatgaacc tgcagaccgc ctcctcgttt gcccgtcgcc gccgcgcgat cgtccagtcg
     5041 ctggccgcgc tgtcgctggg cgccgccttc ctggcgggcg ccccggccgc ccatgccgag
     5101 ggcggcgtgc tgcgcgtctc ggccatcccg gacgaggccc ccagcgagct gcagcgcaag
     5161 ttcaagccgc tgggcgagta cctgaaggcc aggacgggca tggacgtgca gttcacgccg
     5221 gtgaccgact acgccgcggt ggtcgagggc ctggccgcga agaagatcga cctcgcctgg
     5281 ctcggcggct tcaccttcgt gcaggccaag ctgcgcaccg acggccaggt ggtgcccatc
     5341 gtgcagcgcg ccgaggacga gaagttcacg agcaagttca tcgtgccgct ggacagcccg
     5401 atcaagtccg tcgcggacat caagggccac accttcgtgt tcggcgcgcc gtcgtccacc
     5461 tcgggcagcc tgatgccgcg ctacttcctg cagcagcagg gcatcaatcc cgagcgcgac
     5521 ttcaagacgg tcgcgttctc gggcgcgcac gacgccacgg tcgccttcgt ggcctccggc
     5581 cgcgccgagg ccggcgtgct gaactcgtcg gtgatggaca agctggtcga gagcaagaat
     5641 ccgaacgcgc agaaggtgcg cgtgctggcc acgaccccga ccttcttcga ctacaactgg
     5701 acggtgcgcc ctggcatgga tccggccttg cagaagaagc tcaccgacgc gttcctcgcg
     5761 ctcgatccgg ccaggccggc cgacaaggag atcctcgacc tgcagcgcgc gtccaggttc
     5821 gtcccgacca aggcgtcgaa ctacgacggc atcgaggccg ccggcaagtc ggccggcctg
     5881 atcaagtaag gcgcgccgcg tcgatgagcc tgcgcctcga gcgagtcggc gtcgtccatg
     5941 cgaacggccg ccgcgcgctg gccgcggtcg acctcgcggt cgccgcgggc gagcgcgtcg
     6001 cgctgatcgg gccttcgggc gccggcaaga cgacgctgct gcgcgtggcc gcggcctcgc
     6061 tgcggcccac ggacggccac gccagcgtgc tcgaggccga accgtggaca ctgactgcgc
     6121 gcgcgctcaa ggcgctgcgc tcgcgcgttg gattcgtcca ccaggcgccg ccgatcccgc
     6181 cgcgccagcg cgtggtcacc gcggtgctgg ccgggcgtct tggccagtgg agcgtcggcc
     6241 gcgcactgcg ctcgtgggtc gcccccgcgg acgcggccgg cgcgcgtgac gcgctcgcgc
     6301 gcgtgggcct ggccgagcgc gtgttcgagc gctgcgacca gctctcgggc ggccagctgc
     6361 agcgcgtggc cgtggcgcgc acgttgtacc agtcgccgcg gctgctgctg gccgacgaac
     6421 ccgtctcggc cctcgacgcc gccaccgcgg acaccgtgct gcgcgagctc gtcgcctaca
     6481 gcgagtcggc gggcgccacc ttcatcgcgt cgctgcacgc ggtggacctc gcgctgcgct
     6541 ggttcccgcg catcgtcggc gtgcgcgacg gcgcggtgat gttcgacctg ccggccgcgc
     6601 aggtcacgcc cgggcgcctg gccgcgctgt acgcgggcga ggtcgcggcc gagcgcgagc
     6661 ggccggtgca atcgctgccg cacgtcacgc tgccgctggg ttccgcatga ccgccgtcgc
     6721 cgcggtgcgc ccgtcgctgc gcgatccggc gtccagccgg cgcctggcgt gggcgatcac
     6781 cgcgctggtg ctgctgtggc cgctcggacg gctggccgat ttccatcccg cgacgctgct
     6841 ggcgccgtcc aacgtcaagg tgctcggcac gttcctggcc
//
LOCUS       DATCRO010000049         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_821825, whole genome shotgun sequence.
ACCESSION   DATCRO010000049 DATCRO010000000
VERSION     DATCRO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821825"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..715
                     /gene="rdgB"
                     /locus_tag="VIN75_01320"
     CDS             80..715
                     /gene="rdgB"
                     /locus_tag="VIN75_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY8972826.1"
                     /translation="MKLVLASNNAKKLAELQALFEGLRIELVRQGELGVSEAEEPHMT
                     FIENALAKARHASAATGLPAIADDSGLCVDALGGMPGVISANYATVAASAGATLEREV
                     VRQSQDEANNALLLEKLRDVDDRRANFACTLVAIRAADDPSPLVAVGRWNGEILRERE
                     GSGGFGYDPLMFIPAFGATVASLAKEVKNAHSHRAIAAAQMRTLMRQAWHL"
     gene            816..2027
                     /gene="hemW"
                     /locus_tag="VIN75_01325"
     CDS             816..2027
                     /gene="hemW"
                     /locus_tag="VIN75_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY8972827.1"
                     /translation="MRDPQWYHRPGTLSLPAPPPLSVYVHLPWCLKKCPYCDFNSHEF
                     HTVGATPEDLPEQRYLDAVRADLERALPLVWGRTIHTVFIGGGTPSLFSPDGIARLVS
                     DLRMLLPLAADCEITLEANPGTFEKERFRAFAQAGVNRLSIGVQTFNDAHLRALGRVH
                     DAAQARAAAEEAARSFETFNLDLMYALPGQTLADLDADLDLAFALGAPHLSLYHLTLE
                     PNTVFAQRPPANLPDDDTAYDMLDRVSERTASGGFERYEVSAYAKDGHRCRHNVNYWQ
                     FGDYLGLGAGAHGKLSFAHRVTRQVRWREPNMYMERTLAGQAVSNESDVARAQLPFEF
                     MLNALRLRDGFALSLFTERCGLPSSAIAKPLDEAERKGLVERSGAPGAEWIRPTERGF
                     DFLNDLQELFL"
BASE COUNT          356 a          885 c          814 g          327 t
ORIGIN      
        1 ccgtgcgccg ggccgcccca aggaggcccg cggtcccctc gggggacgga gcgccatgcc
       61 cggcgcggga ggggtcgtca tgaaactcgt cctcgcctcc aacaacgcca agaagctcgc
      121 cgaactgcag gcgctgttcg agggcctgcg gatcgagctc gtgcgccagg gcgagctggg
      181 cgtgagcgag gccgaggagc cgcacatgac gttcatcgag aacgcgctgg ccaaggcacg
      241 ccatgcgtcg gcggcgacgg gcctgcccgc gatcgcggac gactcggggc tgtgcgtcga
      301 cgcgctgggc ggcatgccgg gcgtgatctc ggccaactac gccaccgtcg ccgcgagcgc
      361 gggcgccacg ctcgaacgcg aggtcgtgcg ccagtcgcag gacgaggcca acaacgcgct
      421 gctgctcgag aagctgcgcg acgtcgacga tcgccgcgcc aacttcgcct gcacgctggt
      481 ggcgatccgc gccgccgacg acccgtcgcc gctggtggcc gtgggccgct ggaacggcga
      541 gatcctgcgc gagcgcgaag gcagcggcgg cttcggctac gacccgctga tgttcatccc
      601 cgccttcggc gcgacggtgg catcgctcgc caaggaggtg aagaacgcgc acagccatcg
      661 ggcgatcgcc gccgcgcaga tgcgcacgtt gatgcgacag gcgtggcatc tctgagccgg
      721 gttccgtgag catcgtccgc ctccatcccg tcaacgccgc cggcggcgag tccggcgagg
      781 gctcgacgcc cgaatcgtcg ctggccggtg cgccgatccg cgatccgcag tggtaccacc
      841 gccccggcac gctgagcctg ccggcgccgc cgccgctgtc ggtgtacgtg cacctgccct
      901 ggtgcctgaa gaagtgcccg tactgcgact tcaactcgca tgaattccac acggtgggcg
      961 ccacgcccga ggacctgccc gagcagcgct acctggacgc cgtgcgcgcc gacctcgagc
     1021 gcgcgctgcc gctcgtctgg ggccgcacga tccacacggt cttcatcggc ggcggcacgc
     1081 cgtcgctgtt ctcgccggac ggcatcgcgc ggctggtgtc cgacctgcgc atgctgctgc
     1141 cgctggccgc ggactgcgag atcacgctgg aggccaaccc cggcaccttc gagaaggagc
     1201 gcttccgcgc cttcgcgcag gccggcgtga accggctctc gatcggcgtg cagacgttca
     1261 acgacgccca cctgcgcgcg ctcgggcgcg tgcacgacgc ggcgcaggcg cgcgcggcgg
     1321 ctgaagaagc ggcgcgcagc ttcgagacct tcaacctcga cctgatgtac gcgctgcctg
     1381 gccagacgct ggccgacctc gacgccgacc tcgacctcgc gttcgcgctc ggcgcgccgc
     1441 acctgtcgct gtaccacctc acgctggagc ccaacaccgt cttcgcgcag cgcccgccgg
     1501 ccaacctgcc cgacgacgac acggcctacg acatgctcga ccgcgtcagc gagcgcaccg
     1561 cgtccggcgg cttcgagcgc tacgaggtct cggcctacgc gaaggacggc caccgctgcc
     1621 gccacaacgt caactactgg cagttcggcg actacctggg cctgggcgcc ggtgcgcacg
     1681 gcaagctgtc gttcgcccac cgcgtcacgc gccaggtgcg ctggcgcgag ccgaacatgt
     1741 acatggagcg gacactcgcc ggccaggccg tgtccaacga gtccgacgtc gcgcgcgccc
     1801 agctgccctt cgagttcatg ctcaacgcgc tgcgcctgcg cgacggattc gcgctgtcgc
     1861 tgttcaccga gcgctgcggg ctgccgtcct cggccatcgc caagccgctg gacgaggcgg
     1921 agcgcaaggg cctggtcgag cgcagcgggg cgcccggcgc ggagtggatc cggccgacgg
     1981 agcgtgggtt cgacttcctc aacgatctgc aggaactgtt cctgtagagg cgcccaggcg
     2041 cgtccggtgt gcaccgggaa gccgcgccac gggcaggcac gccgattgcc cattggcccg
     2101 aatggtcgag taggcgccgc gctgcggcgg caggggcaaa ccccgcaggg cggggcccgg
     2161 actccaccta tgatcgcgct cgcccgaacg acccgccgcc aggacaccca tgcccgaaaa
     2221 cgcagccatc gacgccgaac agttcaaccg gatcgcgcgc cgcaacgtca ccttgccgct
     2281 ggtcacgggc atcgtcagcg ccgtcgcctt cgtgctgttc ttctggtact tcatcaccgt
     2341 cacgagctgg gtcgaccaca cgcacctggt gatcggcaag ct
//
LOCUS       DATCRO010000050         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_135443, whole genome shotgun sequence.
ACCESSION   DATCRO010000050 DATCRO010000000
VERSION     DATCRO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135443"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VIN75_01330"
     CDS             <1..1135
                     /locus_tag="VIN75_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8972828.1"
                     /translation="PPVAANDLRFEPAVNAAADNLFSQARARSPMLAISGKLARHGLV
                     IDPALESRTGQQTAMTMRMDELIRARVASGSPEFELLPFGEATLPRAQYLLASTATRV
                     DAAPGAPASSARWRLNLSLVELKGGIVLAQSTATALDEGIDTSPTAFYRDSPVLVRDG
                     AIKGYIRTSETAAGQPADRDYLAGVGTAAKLQEATEAYNKAQYADALKQYEVAQAMAN
                     GRQVRTYDGIYLSSTRLDRQAEAEAAFGKLVAAGIADKSLSVKLLFNPGSTTFWSDPQ
                     ISGPYPMWLRQVGAVAGASTVCMDLVGHTSLTGSAAFNDKLSQQRAGVIRERLESGTP
                     SLRGRLVAHGKGSAEPLVGTGTDDARDALDRRVEFRIVDCQPG"
     gene            complement(1107..2036)
                     /locus_tag="VIN75_01335"
     CDS             complement(1107..2036)
                     /locus_tag="VIN75_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8972829.1"
                     /translation="MTLLLYALTVAIWGTTWIALKMQLGIVPIPWSIAYRFALAAVVL
                     LAWLAMRGTLRLPRREVRWMVAAQGLCLFCVNFICFLNASRTIASGLVAVVFSTATLW
                     NALGARLVFKRALTPAILGGGALGLAGLLLMFWPELARHGASAGTVQGLAFALGGTLC
                     FSAGNLLSARMQALGETPALTNAWGMAVGTAVLAIGCAVAGVAPAFDPSTRYVGAWLY
                     LAIPGSVVGFTAYLLLVGRLGPERAAYCTVLFPLVALAISSVVEDYRWTPPALAGLAL
                     VMTGNVLVFRKPKAGATPRAARGALNPAGSPRS"
     gene            2141..3010
                     /locus_tag="VIN75_01340"
     CDS             2141..3010
                     /locus_tag="VIN75_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8972830.1"
                     /translation="MSSNAPSDVFDVLSRSRARLHRRAALGDGLAVAQWSNSFDATRY
                     LPGHHTLSLYLRGGHGTYREDAPSQRGGPGKLCLMPAGHEVNWVVGTGQTFVHLYFAP
                     EQLAPLALRLLDREPRELRLPELNFADDPELALRMASFVALDWTDTADRIAANELGHA
                     VLVDVLARHAGRAPRGGIRGGLAPALRRRLADWLQARMAEPVTVGEMAAFCALSEAHF
                     AHAFRASFGVAPHEWIVARRIERAAQLLRERRALPLDEVARATGHASASHLVRRFKSA
                     WGMTPGQYRAAAA"
     gene            complement(3011..3523)
                     /locus_tag="VIN75_01345"
     CDS             complement(3011..3523)
                     /locus_tag="VIN75_01345"
                     /inference="COORDINATES: protein motif:HMM:NF022152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HEY8972831.1"
                     /translation="MSNELLIHATGLAALALNVVALTCSCERSLRLRSGVSGMIWALN
                     NFLMGANVAAALSVVSAGRTATSAVTLGERRSRRIGFALFAALTLAVGIMTWAGWASL
                     LMVGASLMSTYAVFHLTGRSLRWVMLAVSALWMFNAWSYGSWEQIAANVISAAASLYG
                     ACRVVRPVSA"
     gene            complement(3967..4776)
                     /locus_tag="VIN75_01350"
     CDS             complement(3967..4776)
                     /locus_tag="VIN75_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY8972832.1"
                     /translation="MPDFAVLWPIAVRGLAFSAGLALLTWIASIARRDASLVDRAWSS
                     MIAGPALVYAAQAGWAGPRATALLVLVLAWGLRLCAFITARNWGHGEDRRYQAIRARN
                     EPHFALKSLVLVFALQAVLAWIVSLPTLAALVGERPFGPLDVAGIVVALAGFLFEAIG
                     DAQMSLFKADERNKGRVMDRGLWRYTRHPNYFGEACVWWGVWLVACAAGGVAALWTAV
                     SPLLMTTLLLKVSGVALLEKDIGERRPAYRDYIARTNAFIPGPARRSKHDA"
BASE COUNT          772 a         1832 c         1773 g          605 t
ORIGIN      
        1 gccgcccgtc gccgccaacg acctgcgctt cgagcccgcc gtcaacgcgg cggccgacaa
       61 cctgttctct caggcgcgcg cccggtcgcc catgctcgcg atcagcggca agctcgcgcg
      121 acacgggctc gtcatcgacc cggcgctcga gtcgcgcacg gggcagcaga ccgcgatgac
      181 gatgcgcatg gacgagctga tccgcgcgcg cgtcgcgtcc ggatcgcccg agttcgagct
      241 gctgcccttc ggcgaggcca cgctgcccag ggcgcagtac ctgctggcgt cgacggccac
      301 gcgcgtcgac gccgcgcccg gcgcgccggc gtccagcgcg cgctggcggc tcaacctctc
      361 gctggtggaa ctcaagggcg gcatcgtgct cgcccagagc acggccaccg cgctcgacga
      421 gggcatcgac acgagcccga ccgccttcta ccgcgacagc ccggtgctcg tgcgcgacgg
      481 cgccatcaag ggctacatcc gcaccagcga gaccgcggcc ggccagcccg ccgaccgcga
      541 ctacctggcc ggcgtgggca ccgcggccaa gctgcaggag gcgaccgagg cctacaacaa
      601 ggcgcaatac gccgacgccc tgaagcagta cgaggtcgcc caggccatgg ccaacggcag
      661 gcaggtgcgc acctacgacg gcatctacct gagcagcacg cgcctggatc gccaggccga
      721 ggccgaggcg gccttcggca agctcgtggc cgcgggcatc gcggacaaga gcctgagcgt
      781 caagctgctg ttcaacccgg gcagcacgac gttctggtcc gatccgcaga tcagcggccc
      841 ctatccgatg tggctgcgcc aggtgggcgc ggtggcgggc gcgtcgaccg tctgcatgga
      901 cctggtgggc cacacgagcc tgaccggcag cgccgccttc aacgacaagc tctcgcagca
      961 gcgcgccggc gtcatcaggg agcgcctcga aagcgggacg ccgtcgctgc ggggccgcct
     1021 cgtcgcgcac ggcaagggtt cggcagaacc gctggtgggc accgggacgg acgacgcccg
     1081 cgacgcgctg gaccggcgcg tggagttcag gatcgtggac tgccagccgg gttgagcgcg
     1141 ccccgcgccg cccgcggcgt cgcccccgcc ttcggcttgc ggaacaccag cacgttgccc
     1201 gtcatcacca gcgccaggcc ggcgagcgcg ggcggcgtcc agcggtagtc ctcgacgacc
     1261 gacgagatgg ccagcgccac cagcgggaac agcaccgtgc agtaggccgc gcgctcgggg
     1321 ccgaggcggc cgaccagcag caggtaggcc gtgaagccga ccaccgagcc gggaatcgcc
     1381 aggtacagcc acgcgccgac gtagcgcgtc gacggatcga acgccggcgc gacgccggcc
     1441 acggcgcagc cgatcgccag caccgcggtg cccaccgcca tgccccatgc attggtgagc
     1501 gcgggcgtct cgccgagcgc ctgcatgcgg gccgacagca ggttgccggc cgagaagcac
     1561 agtgtgccgc cgagcgcgaa cgcgaggccc tgcacggtgc cggcggacgc gccgtggcgc
     1621 gccagctcgg gccagaacat cagcagcagg ccggccaggc ccagcgcgcc gccgccgagg
     1681 atggccggcg tcagcgcgcg cttgaacacg agccgcgcgc cgagcgcgtt ccatagcgtg
     1741 gccgtcgaga acaccaccgc cacgagcccg ctggcgatcg tgcggctcgc gttgagaaag
     1801 cagatgaagt tgacgcagaa caggcacaag ccctgcgccg cgaccatcca gcgcacctcg
     1861 cgccgcggca gccgcagcgt gccgcgcatc gccagccagg caagcagcac gacggccgcc
     1921 agcgcgaagc ggtacgcgat cgaccagggg atcggcacga tgcccagctg catcttgagt
     1981 gcgatccagg tcgtgcccca gatggcgacg gtcagcgcgt agagaagcaa ggtcatggcg
     2041 cgagtctgcg cgcgccggcg gcgcgccgat tgcacgttcc tgcgcttcgt cgccgcgcat
     2101 gcgcgcgcgg gcgcgtcacg cgcgctacag tcccatcgcc atgtccagca acgccccgtc
     2161 cgacgtcttc gacgtgctct cgcgctcgcg cgcgcgcctg caccggcgcg cggccctcgg
     2221 cgacgggctg gccgtggcgc aatggtccaa cagcttcgac gccacgcgct acctgcccgg
     2281 gcaccacacg ctgtcgctgt acctgcgcgg cggccacggg acctatcgag aggacgcgcc
     2341 cagccagcgc ggcggccccg gcaagctgtg cctgatgccg gcgggccacg aggtcaactg
     2401 ggtggtgggc accgggcaga ccttcgtgca cctgtacttc gcgcccgagc agctggcgcc
     2461 gctggcgctg cggctgctcg atcgcgagcc gcgcgagctg cggctgcccg agctgaactt
     2521 cgccgacgac cccgagctcg ccctgcgcat ggccagcttc gtcgcgctgg actggacgga
     2581 caccgccgac cgcatcgctg ccaacgagct cggccacgcg gtgctggtcg acgtgctggc
     2641 gcgccacgcg ggacgcgcgc cgcgcggggg catccgcggc ggcctggcgc cggcgctgcg
     2701 gcggcgcctg gccgactggc tgcaggcgcg catggccgag ccggtgacgg tgggcgagat
     2761 ggccgcgttc tgcgcgctgt ccgaagcgca tttcgcgcat gcgttccgcg cgtcgttcgg
     2821 cgtcgcgcca cacgagtgga tcgtcgcgcg ccgcatcgag cgcgccgcgc agctgctgcg
     2881 cgagcgccgc gcgctgccgc tggacgaggt cgcccgcgcg acgggccacg cgagcgccag
     2941 ccacctggtg cggcgcttca agtcggcctg gggcatgacg cccgggcagt accgggcggc
     3001 ggcggcctga tcaggcgctc accggccgca cgacgcggca ggcgccgtaa agcgacgcgg
     3061 cggcactgat cacgttggcc gcgatctgct cccacgagcc gtagctccac gcgttgaaca
     3121 tccacaacgc ggacacggcg agcatcaccc agcgcagcga gcggccggtg aggtggaaga
     3181 cggcgtaggt cgacatcagc gaggccccga ccatcagcag cgacgcccag ccggcccacg
     3241 tcatgatgcc gacggccagc gtcagcgcgg cgaacagcgc aaagccgatg cgacgcgacc
     3301 ggcgctcgcc cagcgtgacg gccgacgtcg cggttcggcc ggcgctcacg acgctcagcg
     3361 cggcggcgac gttggcgccc atcaggaaat tgttgagcgc ccagatcatt cccgagacgc
     3421 ctgaccgcaa gcgcagcgac cgctcgcagg agcaggtcag cgcgacgacg ttcagggcca
     3481 gcgcggcgag gccggtggca tggatgagca gctcgttgga catggcgggt tgtccatgga
     3541 gtctaggaat cgcgtcggat cgcgatgggc ggatcagcgg ggcgtccctg gcgctaaacg
     3601 cagttggtta cgaggctgca cggctgttgc gcaactgttc ctggcctgtt ggtcgagtgt
     3661 tcctgggagg caatgcccgc ggccaggcgc cttgcctgcc agcggacatg acccgaacat
     3721 cgggccggca cgcaggcgct tccttctcgt gatcgacgaa ggcgcgtctt cgcggatggc
     3781 gctgcgtagg gcgggttagc cgcaggcgta acccgccgtt cgccggcgtg cagatcggtt
     3841 atcgcggaaa tgccgggccg tgcgccacgt agcggcgggt tacgccagcg gcgaacccgc
     3901 cctacacggc tgctcggtgc gcagattggt gttcccaggt ggcgaacgcc attgcgacga
     3961 gcgacttcac gcgtcgtgct tcgagcgccg cgccggccct ggaatgaacg cgttcgtccg
     4021 cgcgatgtag tcgcgatacg ccggccgccg ctcgccgatg tccttctcca gcagcgcgac
     4081 gcccgacacc ttcagcagca acgtcgtcat cagcagcggc gacaccgccg tccacagcgc
     4141 cgcgacgccg cccgccgcgc aggcgaccag ccagacgccc caccagacgc acgcctcgcc
     4201 gaagtagttc ggatgccggg tgtagcgcca caggccgcga tccatcaccc gccccttgtt
     4261 gcgctcgtcg gccttgaaga gcgacatctg cgcgtcgccg atcgcctcga acaggaatcc
     4321 cgcgagcgcg acaacgatgc cggcgacgtc cagcggcccg aacggccgct cgccgacgag
     4381 cgcggccagc gtcggcagcg agacgatcca tgcgagcacc gcctgcagcg cgaacacgag
     4441 cacgaggctc ttgagcgcga agtgcggctc gttgcgcgcg cggatcgcct ggtagcgacg
     4501 atcctcgccg tgcccccagt tgcgcgccgt gatgaaggcg cacaagcgca gtccccaggc
     4561 cagcaccagc acgagcagcg cggtcgcgcg cggccccgcc catccggcct gcgcggcgta
     4621 gacgagcgcc gggccggcga tcatcgacga ccaggcgcgg tcgaccaggc tcgcgtcgcg
     4681 ccgcgcgatg ctcgcgatcc acgtgagcag cgccaggcct gcgctgaagg ccaggccgcg
     4741 gacggcgatg ggccacagga cggcgaaatc aggcatggtc gttccggttg gtgacagcgt
     4801 ccaggcgctc gggcaccggc gtcagcagcg gcatcaggac cgcccacgtc gcggcgaggt
     4861 tggcgagcat gcgagcgcgc gagggcccgc ctgcgtcacg cgcgtcgcgc tctcgcatcc
     4921 gcggcggcgc gtgggcggcg ttcacgcgcg cttctcgaac agatagtggc tcacccgcca
     4981 tt
//
LOCUS       DATCRO010000051         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_158141, whole genome shotgun sequence.
ACCESSION   DATCRO010000051 DATCRO010000000
VERSION     DATCRO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158141"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VIN75_01355"
     CDS             <1..766
                     /locus_tag="VIN75_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY8972833.1"
                     /translation="SAIAGPTRPPFAWPPATRLTYTLTGERGVGVHIYGNAVVEWRHE
                     GSHYQVQFDVHISPMIDQHMYSEGEITDDGLSPKHYDESFSFLFSTPRNRRVEFGDTD
                     VRLNNGERVYKLKQTQDGASQFVQFVWMFYNNPEWLKPGVIVHIPLALPNNLRRWNYQ
                     VVGTERLDMAFGPIDAVHLKPLLDGPRRPNEYPFDIWTAPGLQYLPVQILVKVDEHTW
                     ALLALDDLPLQAAGERPPPPPPRTNPFPPPPPFGER"
     gene            822..1610
                     /locus_tag="VIN75_01360"
     CDS             822..1610
                     /locus_tag="VIN75_01360"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY8972834.1"
                     /translation="MTYECILTETVGNGPLKTGVIRLNRPKQMNALNDQLMDELGAAL
                     LAFDADDGIGAIVLTGNERAFAAGADISVMAKYSYMDAFMKGHISRNWETMRQVRKPV
                     IAAVAGFALGGGCEVAMMCDMMIAADTAKFGQPEIKLGIVPGAGGTQRLPRAIGKAKA
                     MDMVLTGRLMDAAEAERSGLVSRVVPAVQLMTEALAVAEQLCGLGMPSVMLAKDAVNR
                     SFETGLTDGIAYERRLFHSLFGTQDQQEGMAAFLEKRKPEFKNR"
     gene            complement(1597..2964)
                     /locus_tag="VIN75_01365"
     CDS             complement(1597..2964)
                     /locus_tag="VIN75_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972835.1"
                     /translation="MPDLRPSRWPARALSTLALTAILAGCTTPPAPVAQAPKPVHQIP
                     AGARQALAQRFPGRRIVADSHGPLLAADVDAVAVVLDGDAPGAFVVAVLAPPAAAGAD
                     WRVIGTSRPIVPGCAKCSVSADLAPHGLYVRVIRAEGAGFENFTYQFAGTEGGDPLRL
                     VGVTAYIPSRADDPSSHSFSASVDLLTGQRTDIVEESGSDGATTHRERQGSVPLRAPI
                     AFDDFAFTAEALDAETRRSPPVAFDPAGTLPAAAVDALRERFPRMTVQSQSSGALRGE
                     GRDIVAVLVPADRAARAGAAADAVVAVLLAQPDGSLHLADVSGAMAHACPTCDVQVQV
                     ARHTLSVETTEVTPTGSVSTDWQFAFRPKDAPLRLVGLRTETTVRGDDGTGRRSTVTS
                     TNLVNGDRVEVTDSLVNGRRRRNEQKSRLQVRAPVLLSDFAFDPSVLGDDIAAGTDVA
                     RLSGS"
     gene            complement(3006..4346)
                     /locus_tag="VIN75_01370"
     CDS             complement(3006..4346)
                     /locus_tag="VIN75_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972836.1"
                     /translation="MRFPDPDRLRLAAPAAFLAALLAGCATHAPAPAPAPEPRRVEQP
                     TDTGLPAGAARALAQRFPALHPVGHSAGHLQVDDADDLAVVLAPPGQSSEPIVAVLVT
                     GAAGEYRIATASKPVAPGCDTCTTTIDIAHKLLSVHVMRPSDPDFERVTWQFGYTDDA
                     LRLVNVTAAQPAGDDPIAHGYAISTNLLTGAKVDTLDPTQADPSRRRELKSSVPLRRG
                     IAFDAFDFTPQALGPELRRLPASAFEPQDALPASAVALLRKSFPGATVTGRSTGPLRP
                     DGPRDLAVVLDPAGNGDADTTLALLLAQPDGGFRLGATSGPLTRNCRGCDVQVQIAHH
                     ALVVQTASVDGGTTRIVGWQFMAVPKDKAGALRLVGVRTVLASRSGADSHRYVNTANL
                     LTGDKLDVVEDVVRGHRKRAEHPSKVAVRPPIPFAGFAFDASKLDAETRRDFTP"
     gene            complement(4649..5233)
                     /locus_tag="VIN75_01375"
     CDS             complement(4649..5233)
                     /locus_tag="VIN75_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8972837.1"
                     /translation="MTTRLQELDEIEASVWSELERCVAARPRSERGEDAQEAPHEWRV
                     AVLATVDDGQADARSLVLREVDAAERRLIFYTDARSPKVRQIEKSRQGMLIFYSRELG
                     WQLRMQVRLNVETTGLAVSSRWAKLQTSAGARDYLSVAAPGRPLDAPLSELGVRGHFA
                     MIEAMAETVDWLEVHPRGHRRAVLGGDRSHWVQP"
BASE COUNT          787 a         1961 c         2070 g          750 t
ORIGIN      
        1 gtcggcgatc gccggcccga cgcgcccgcc gttcgcctgg ccgccggcca cgcgcctgac
       61 ctacacgctg actggcgaac gcggcgtcgg cgtgcacatc tacggcaacg cggtggtcga
      121 gtggcgccac gagggctcgc actaccaggt gcagttcgac gtgcacatct cgccgatgat
      181 cgaccagcac atgtacagcg agggcgagat caccgacgac ggcctcagcc cgaagcacta
      241 cgacgagtcc ttcagcttcc tgttctcgac gccgcgcaat cgccgggtcg agttcggcga
      301 caccgacgtg cggctcaaca acggcgagcg cgtctacaag ctcaagcaga cgcaggacgg
      361 cgccagccag ttcgtgcagt tcgtctggat gttctacaac aaccccgagt ggctcaagcc
      421 cggcgtcatc gtgcacatcc cgctggcgct gccgaacaac ctgcggcgct ggaactacca
      481 ggtggtgggc accgagcggc tggacatggc gttcggcccc atcgatgccg tgcacctgaa
      541 acccctgctg gacggcccgc gccggccgaa cgaatatcct ttcgacatct ggacggcgcc
      601 cggcctgcaa tacctgccgg tgcagatcct cgtgaaggtc gacgagcaca cctgggcgct
      661 gctggcgctg gacgacctgc cgctgcaggc cgccggcgag cgcccgccgc cgccaccgcc
      721 caggacgaac ccgttcccgc cgccgccgcc gttcggcgaa cgctgagccg gcccacgaag
      781 ccgcgccatc gcaccgattg cccaccttca aggagacccc catgacctac gaatgcatcc
      841 tgaccgagac cgtcggcaac ggtccgctca agacgggcgt gatccggctg aaccggccca
      901 agcagatgaa cgcgctcaac gaccagctga tggacgagct cggcgccgcg ctgctggcct
      961 tcgacgccga tgacggcatc ggcgcgatcg tgctgaccgg caacgagcgc gcgttcgccg
     1021 ccggcgccga catctcggtc atggccaagt acagctacat ggacgcgttc atgaagggcc
     1081 acatctcgcg caactgggag acgatgcgcc aggtgcgcaa gcccgtcatc gccgcggtgg
     1141 ccgggttcgc gctgggcggc ggctgcgagg tggcgatgat gtgcgacatg atgatcgccg
     1201 ccgacaccgc caagttcggc cagcccgaga tcaagctggg catcgtgccc ggcgccggcg
     1261 ggacgcaacg cctgccgcgc gcgatcggca aggccaaggc gatggacatg gtgctgaccg
     1321 gccgcctgat ggacgccgcc gaggccgagc gctccggcct ggtctcgcgc gtggtgccgg
     1381 ccgtccagct gatgaccgag gcgctggccg tcgccgagca gctgtgcggc ctgggcatgc
     1441 ccagcgtgat gctcgccaag gacgcggtca accgctcgtt cgagacgggc ctgaccgacg
     1501 gcatcgccta cgagcgccgc ctgttccact cgctgttcgg cacgcaggac cagcaggaag
     1561 gcatggccgc cttcctcgag aagcgcaagc cggaattcaa gaaccgctga gcctggcgac
     1621 gtccgtcccg gcggcgatgt cgtcgccgag gacggacggg tcgaacgcga aatccgacag
     1681 cagcacgggc gcgcgcacct gcagccgcga cttctgctcg ttgcggcgcc ggcggccgtt
     1741 gacgaggctg tccgtcacct cgacgcggtc gccgttgacc aggttggtgc tggtcaccgt
     1801 gctgcggcgt cccgtgccgt cgtcgccgcg caccgtcgtc tcggtgcgca ggcccaccag
     1861 gcgcagcggc gcatccttcg ggcggaacgc gaactgccag tcggtcgaca cgctgcccgt
     1921 gggcgtgacc tcggtcgtct cgacgctgag cgtgtggcgc gcgacctgca cctgcacgtc
     1981 gcaggtcggg caggcgtgcg ccatcgcacc cgacacgtcg gccaggtgca ggctgccgtc
     2041 gggctgcgca aggaggaccg cgacgacggc gtcggccgcg gcaccggcgc gcgcggcgcg
     2101 gtcggcggga acgagcacgg ccacgatgtc gcggccctcg ccgcgcagcg cgccgctcga
     2161 ttgcgactgc accgtcatgc gcgggaagcg ctcgcgcagc gcgtcgaccg ccgccgcggg
     2221 cagcgtgccg gcggggtcga aggcgacggg cgggctgcgg cgcgtctcgg cgtcgagcgc
     2281 ctcggcggtg aacgcgaagt cgtcgaacgc aatgggcgcg cgcagcggca cgctgccctg
     2341 gcgctcgcgg tgcgtggtgg cgccgtcgct gccggactcc tcgacgatgt cggtgcgctg
     2401 gcccgtgagc aggtcgacgc tggccgagaa gctgtgcgac gacgggtcgt cggcgcgcga
     2461 cgggatgtac gcggtcacgc ccaccaggcg cagcggatcg ccgccctcgg tgccggcgaa
     2521 ctggtaggtg aagttctcga agccggcgcc ctcggcgcgg atcacgcgca cgtacaggcc
     2581 gtgcggcgcc aggtcggcgc tgacgctgca cttggcgcag ccgggcacga tgggccggga
     2641 cgttccgatc acgcgccagt cggcgccggc ggccgcgggc ggggccagca ccgcgaccac
     2701 gaacgcccct ggcgcgtcgc cgtccagcac gacggcgacg gcgtcgacat cggcggccag
     2761 cagcgggccg tggctgtcgg cgacgatgcg gcggcccggg aaacgctgcg ccaacgcctg
     2821 cctggcgccg gcggggatct ggtggacggg cttgggggcc tgggcgacgg gcgccggcgg
     2881 cgtcgtgcag ccggcaagaa ttgccgtcag ggccagcgtg gagagggcgc gcgcgggcca
     2941 gcgcgagggt cggagatccg gcatgggcgc ggccgggcag gcgtcatgcc cagcccggcc
     3001 ctggatcagg gggtgaagtc gcgccgcgtc tcggcgtcga gcttgctcgc gtcgaacgcg
     3061 aagcccgcga acgggatcgg cggtcggacg gccaccttgc tcggatgctc ggcgcgcttg
     3121 cggtggccgc gcaccacgtc ctcgacgacg tcgagcttgt cgccggtgag caggttggcg
     3181 gtgttgacgt agcggtggct gtcggcgccg ctgcggctgg ccagcacggt gcgcacgccg
     3241 accaggcgca gcgcgccggc cttgtccttg ggcacggcca tgaactgcca gccgacgatg
     3301 cgcgtggtgc cgccgtcgac gctcgccgtc tgcacgacca gcgcgtggtg cgcgatctgc
     3361 acctgcacgt cgcagccgcg gcagttgcgc gtcagcgggc cgctggtggc gccgagacgg
     3421 aagccgccgt cgggctgcgc gagcagcagc gccagcgtcg tgtcggcgtc gccgttgccg
     3481 gcggggtcga gcacgacggc caggtcgcgc gggccgtccg gccgcagcgg gccggtgctg
     3541 cggcccgtca cggtcgcgcc ggggaagctc ttgcgcagca gcgcgacggc cgaggccggc
     3601 agcgcgtcct ggggctcgaa ggccgacgcc ggcaggcgcc gcagttcggg gccgagggcc
     3661 tgcggcgtga agtcgaacgc gtcgaaggcg atgccgcggc gcagcggcac gctggacttg
     3721 agctcgcgcc gccgcgacgg atcggcctgc gtcgggtcga gcgtgtcgac cttggcgccg
     3781 gtgagcaggt tcgtcgagat cgcgtagccg tgcgcgatcg ggtcgtcgcc cgcgggctgc
     3841 gcggccgtga cgttgaccag gcgcagcgcg tcgtcggtgt agccgaactg ccaggtgacg
     3901 cgctcgaagt ccgggtcgga cggccgcatc acgtggacgg acagcagctt gtgggcgatg
     3961 tctatggtcg tggtgcaggt gtcgcagccg ggcgcgaccg gcttgctggc cgtggcgatg
     4021 cggtactcgc cggcggcgcc cgtcaccagc acggcgacga tgggctcgct ggactggccc
     4081 ggcggcgcga ggacgacggc caggtcgtcg gcatcgtcca cctgcaggtg gcccgcactg
     4141 tggccgaccg gatgcagggc agggaagcgc tgggccagcg cgcgtgctgc gcccgcgggc
     4201 aggccggtgt cggtcggctg ctcgacccgg cgtggttcgg gcgccggtgc gggcgcgggg
     4261 gcgtgggtgg cgcagcctgc gagcagggcg gcgaggaagg ccgccggcgc ggccagcctg
     4321 agtcgatcgg gatccggaaa tcgcatgccc ggatcatgcc ctttcgcgcg acgcggatcg
     4381 cgtaacgggg cgtatccgtc ctggggatga gagccacgcc gttcaccccg gcgccaccgt
     4441 ggatcctgcg actacgcgca ggatgacgac ttgactggcg tgtccgcgag ccgcgccgtc
     4501 attccgcgcg cagtcgcgga gtccacggtg gcgacgaggt gcggcaagcg gtgcagcgcg
     4561 agtcgccggg cgggcccgga agtgcgtcga tcggcgctgt tggcgaaacc cgcggcgctc
     4621 gccaacgcgc cagcgccgcc gccacccgtc agggctgcac ccaatgcgac cggtcgccgc
     4681 ccagcaccgc gcggcgatgc ccgcgcggat gcacctcgag ccagtccacc gtctcggcca
     4741 tggcctcgat catcgcgaag tggccgcgca cgcccagttc cgacagcggc gcgtcgagcg
     4801 gacggcccgg ggcggcgacg gacaggtagt cgcgtgcgcc ggcggacgtc tgcagcttgg
     4861 cccagcgcga ggacacggcc aggccggtcg tctccacgtt gagccgcacc tgcatccgca
     4921 gttgccagcc gagctcgcgg ctgtagaaga tcagcatgcc ctgccgcgac ttctcgatct
     4981 ggcgcacctt gggcgagcgc gcgtcggtgt agaagatcag ccgccgctcg gccgcgtcca
     5041 cctcgcgcag caccaggctg cgggcgtcgg cctggccgtc gtcgacggtg gccagcacgg
     5101 cgacgcgcca ctcgtgcggc gcctcctggg catcctcgcc gcgctcgctg cgcggccgcg
     5161 ccgccacgca tcgttcgagc tcgctccaga cgctcgcctc gatctcgtcg agttcctgca
     5221 atcgtgttgt cacggcagac ctccgggcgg gaaggggaga cccaacgcta acgctcgcca
     5281 ccgcgccggt caatcgcccg cgcgtaaatt ttctcgatcg attttcgatc gatcgctcgc
     5341 gccggcgata cgcaagaaag ctcgcgcgaa tgcggcgtta aggtgacgtc aacgcggcgc
     5401 ggcgatggcg cactgcaaca cgcgagttgc ccggagcgag atacggcatc aggtatacct
     5461 ccggagcgct tgcccgctgc cgcgtcagcg cttcgacgcc gccggcgctg ccgctcgaat
     5521 gtcgccatcg agattcgagg gagcgacgga tccggcttcg ccgggccg
//
LOCUS       DATCRO010000052         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_551865, whole genome shotgun sequence.
ACCESSION   DATCRO010000052 DATCRO010000000
VERSION     DATCRO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551865"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VIN75_01380"
     CDS             <1..1020
                     /locus_tag="VIN75_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8972838.1"
                     /translation="IQDTESMRERLATTIGTVRNSVDSIGTASTEIATGNLDLSQRTE
                     QTASNLQQTASSLSQLTANVRQSAEAASQANQLASSASSVASRGGEVVAQVVSTMDEI
                     NASSKKIADIIGTIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRSAE
                     AAKEIKSLIGTSVDKVETGSKLVQDAGSTMDEIVASVQRVTDIIGEISAAALEQSQGI
                     GQVNEAVTRLDQMTQQNAALVEESAAAAESLKEQAHRMTTVVATYRLESGMDIGFAAA
                     PRHQAAATKAIAKAAAPAARPVAAAKPVAPKAAPAPVAAAPAVVPAPVAPHAASGASD
                     DWESF"
     gene            1194..1709
                     /locus_tag="VIN75_01385"
     CDS             1194..1709
                     /locus_tag="VIN75_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8972839.1"
                     /translation="MDMTKEATHIARNDAARHGQVAGTKGEFLTFRLGGEEYGIDILR
                     VQEIRSYEQPTRIANTPEFLRGVVNLRGVIVPIVDLRLKLGCTAEFNAFTVVIVLNVK
                     GRVIGAVVDSVSDVCELGGDMIKPAPLMNSGVDMSFITGIANVGERMLILMDIEGLMS
                     ASDMGLIEAAA"
BASE COUNT          385 a          809 c          711 g          270 t
ORIGIN      
        1 atccaggaca ccgagagcat gcgcgagcgc ctggccacga cgattggcac ggtccgcaac
       61 tcggtcgaca gcataggcac cgcgagcacc gagatcgcca ccggcaacct cgacctcagc
      121 cagcgcaccg agcagaccgc cagcaacctg cagcagacgg ccagctcgct gtcgcagctg
      181 accgccaacg tgcgccagag cgccgaggcc gcctcgcagg ccaaccagct cgcgagctcc
      241 gcctcgtcgg tcgccagccg cggcggcgaa gtcgtggccc aggtcgtctc gaccatggac
      301 gagatcaacg ccagctcgaa gaagatcgcc gacatcatcg gcacgatcga cggcatcgcc
      361 ttccagacca acatcctcgc gctgaacgcg gccgtcgaag cggcgcgcgc cggcgaacag
      421 ggccgcggct tcgcggtcgt ggccggcgag gtgcgctcgc tggcgcagcg ctcggccgag
      481 gccgccaagg aaatcaagag cctgatcggc acctcggtcg acaaggtcga gaccggttcg
      541 aagctggtgc aggacgccgg ctccacgatg gacgagatcg tcgcgagcgt gcagcgcgtg
      601 accgacatca tcggcgagat cagcgccgcc gccctcgagc agtcgcaggg catcggccag
      661 gtcaacgagg ccgtcacgcg gctcgaccag atgacgcagc agaacgccgc gctggtggag
      721 gaatccgccg ccgccgccga gagcctgaag gaacaggcgc accgcatgac cacggtcgtg
      781 gccacctacc gcctcgagag cggcatggac atcggctttg cggccgcgcc ccgacaccag
      841 gccgccgcca ccaaggcgat cgccaaggcc gccgcaccgg ccgccaggcc cgtcgccgcc
      901 gccaagccgg tcgcgcccaa ggccgcgccg gcgccggtcg ccgccgcccc cgccgtcgtc
      961 ccggctcccg tcgccccgca cgccgcctcc ggcgccagcg acgactggga atcgttctga
     1021 tcccgcactg aacggagagg gcgcgtcacg gcgccctttt tttcgcccga tcccaccggc
     1081 gcgccccgga aacggcgcaa aaagtcactt cattccgcgc ggccgtggcc gataacggaa
     1141 acaagaagcc ctccgcctcc ccaccgactt caagaaaccc gcaaggatcc gccatggaca
     1201 tgaccaagga agccacccac atcgcgcgca acgacgcagc gcgccacggc caagtcgccg
     1261 gcaccaaggg cgaattcctg accttccgcc tgggcggcga ggagtacggc atcgacatcc
     1321 tgcgcgtgca ggagatccga tcctacgagc agccgacccg catcgccaac acgcccgagt
     1381 tcctgcgcgg cgtcgtcaac ctgcgcggcg tgatcgtgcc gatcgtcgac ctgcgcctga
     1441 agctgggctg caccgccgaa ttcaacgcgt tcacggtcgt catcgtcctg aacgtcaagg
     1501 gtcgcgtgat cggcgccgtc gtcgactccg tgtcggacgt ctgcgaactg ggcggcgaca
     1561 tgatcaagcc ggccccgctg atgaactccg gcgtcgacat gtccttcatc acgggcatcg
     1621 ccaacgtcgg cgagcgcatg ctgatcctga tggacatcga ggggctcatg agcgcgtccg
     1681 acatggggct gatcgaagcg gccgcctgag cgtcccgctt ccgatgcgaa cagccgcctc
     1741 cgggcggctt tgtcgtttcg gcatggggat gcctcgaggg gtctggcctt tccgcgctcc
     1801 gcgcaatcaa cgccgaccag cgcgacgagg ccctccgcgc agagtggaca ggcctgaccc
     1861 cgaacgtcag cgaagcgggg tcgggcctgt cctcgaatcg cggccggcgc cagcgcttcg
     1921 acggcattcc ggcgcgattc gaggaaaggc cagacccctt cgacggccga gcgtggcacg
     1981 cgcgccatga ggacgccgcg gggcacgcag ctcaggcgaa gatgcgtgcc gccggccgcg
     2041 ccgtgccatc cgccacgacg cgcacgaccg cgcgggcgat cttcgcgccg ccgacgacgg
     2101 acggctcgat cgggttggcg tagtcctgcg gcaggtcgca gacggcccgc aggtcgatca
     2161 cgtccagccc gtggg
//
LOCUS       DATCRO010000053        12902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_461921, whole genome shotgun sequence.
ACCESSION   DATCRO010000053 DATCRO010000000
VERSION     DATCRO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461921"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /gene="rpoD"
                     /locus_tag="VIN75_01390"
     CDS             <1..1698
                     /gene="rpoD"
                     /locus_tag="VIN75_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8972840.1"
                     /translation="SMLNDMGIAVYEQAPDAATLLISGQTQSGATEEEAEEAAEAALS
                     TVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEGGLQAMMQAISASPTTIAE
                     ILLVAERIGKGEMQISEAVDGFVSNDEADDYVAEEDFDEFDEEDDDDGAGGSKALTKK
                     LEELKAQALTKFGELSAHFDKMRKAFEKDGYRSPSYDKAQEALTGDLMKIRFTVKTIE
                     RLCSILRGQVDDVRRYEREIRKIVVDKCGMPQEHFIKTFPANSLNLHWAEKEIAGNRA
                     YSVVMSRNLPAIQELQQKLIDLQARAVVPIEHLKDINRKMNEGEKASRDAKKEMIEAN
                     LRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITR
                     SIADQARTIRIPVHMIETINKMNRISRQHLQEFGFEPDAPTLAEKMEMPEDKIRKIMK
                     IAKEPISMETPIGDDDDSHLGDFIEDTNNLAPVEAAMHAGLRDVVKDILDSLTPREAK
                     VLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKAIRKLKHPSRSDKLRTYLDNL"
     gene            1880..2863
                     /locus_tag="VIN75_01395"
     CDS             1880..2863
                     /locus_tag="VIN75_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8972841.1"
                     /translation="MSAIQRRAVVFADLRGSTALYELLGNTDASQIVTETVDAISRRV
                     PATGGVVVKTLGDGLMAVFPTAAQAVEAADQMHEEIDIVMRRRDALTGFTEARLQLQV
                     CIALGETVEVSGDYFGDAVNVAARLLDHAGDNETLVTADVLRALPLDSRDMFRSLDLV
                     RLRGRVEPVEVHLLSRRGMDTAPTLVGGGPLLGMAGPDAVILTWGNKVRRFTATDMPV
                     VIGRAAASDMQVDDARVSRSHCRIDLIGATLQLSDLSINGTFVRFTSEDEVLSLRRGS
                     CTLHGSGEVGLSGSPEDPGVAAMQFEVLHAPLRRDDEYDLLSPIIAPPSRR"
     gene            2860..3945
                     /gene="hemH"
                     /locus_tag="VIN75_01400"
     CDS             2860..3945
                     /gene="hemH"
                     /locus_tag="VIN75_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8972842.1"
                     /translation="MSAAPRAHAHDGGDKVGVLLVNLGTPEAPTTPALRRYLAQFLSD
                     PRVVEIPRALWWLILHAVILRVRPRSSAAKYRKIWLREGSPLAVWTARQANMLRSYVG
                     ERGALVEVRWAMRYGQPAIAAELDALCAAGCTRILVLPAYPQYSAATTASALDDVGAW
                     MRGTRRLPELRFVNHYHDDPGYIEALAAQVRKHWQREGRGRKLVMSFHGMPARTLQLG
                     DPYHCECLKTARLLAEKLQLDDDAWRVTFQSRFGRAEWLQPYTEPTLRALAKAGAEGV
                     DVICPGFAADCLETLEEIAIEAKAAFLAAGGKTFHYIPCLNNSPEGMKAMAGVALRHL
                     QGWDTTPMSTEALQQRRARAIASGASE"
     gene            3949..4392
                     /locus_tag="VIN75_01405"
     CDS             3949..4392
                     /locus_tag="VIN75_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY8972843.1"
                     /translation="MSRHTAPSEAPAAAADMRLDKWLWVARFYKTRGLAAEEVEKGRV
                     QVNGQAAKPARSVRVNDRIEIRQPGTVRELLVLGLGAMRGPATVARTLYQETEASIRA
                     AQQAAEARKFGVEPAHAQETGRPTKRDRRDLVDWQRWSASVDDDR"
     gene            4508..5047
                     /gene="grpE"
                     /locus_tag="VIN75_01410"
     CDS             4508..5047
                     /gene="grpE"
                     /locus_tag="VIN75_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8972844.1"
                     /translation="MNTEDQTPPSQQQPPADDGAAHLDIGLGGDAARIAELEAKVAEL
                     TDASLRAQAEAQNARRRADEEVAKARKFAVEGFADSLLPVKDSLEAAIATPGAAVEQV
                     LEGVHATLRQLSAALERNKVAQINPPAGTKFDPHQHQAISMVPAEQEANTVVTVLQKG
                     YLIADRVLRPALVTVSAPK"
     gene            5187..7136
                     /gene="dnaK"
                     /locus_tag="VIN75_01415"
     CDS             5187..7136
                     /gene="dnaK"
                     /locus_tag="VIN75_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8972845.1"
                     /translation="MGRIIGIDLGTTNSCVSIMEGNSTKVIENSEGARTTPSIIAYQE
                     DGEVLVGASAKRQAVTNPKNTLYAVKRLIGRKFTEKEVQKDIDLMPYKITAADNGDAW
                     VEVRGKKLAPQQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQGKGDRKIAVYDLGGGTFDISIIEIADVDGEMQFE
                     VLSTNGDTFLGGEDFDQRIIDFIVSEFKKEQGVDLTKDVLALQRLKEAAEKAKIELSN
                     QTQTDVNLPYITADASGPKHLNIKLTRAKLESLVEDLIERTIAPCRTAINDAGVKTGD
                     INDVILVGGMTRMPKVQDKVKEFFGKDPRKDVNPDEAVAVGAAIQGQVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMIKKNTTIPTKFSQVFSTADDNQPAVTIKVYQGERELAS
                     GNKSLGEFNLEGIPPAPRGTPQIEVTFDIDANGILHVGAKDKATGKENKITIKANSGL
                     SEDEIQKMVRDAEINAAEDAKKVELVSARNQGDAMVHSVRKSLGEHGDKLEASEKQAI
                     ETALKDVEEAIKGDDKAAIEAKTEALMTASQKLGEKVYADQQAAQAAAGAAAGGGAQA
                     QAEPQAAHAADDNVVDADFKEVKKS"
     gene            7298..8431
                     /gene="dnaJ"
                     /locus_tag="VIN75_01420"
     CDS             7298..8431
                     /gene="dnaJ"
                     /locus_tag="VIN75_01420"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8972846.1"
                     /translation="MSKRDYYEVLGLTKGASDDDIKKAYRKLAMKHHPDRNQGDGAKA
                     AEEKFKEAKEAYEMLSDPQKKAAYDQYGHAGVDPNMGHGAGEGFGGFAEAFGDIFGDI
                     FGGGRRPGGGGGKQVYRGNDLSYAMEITLEEAAAGKDTQIRVPSWENCETCKGSGAKP
                     GTSPKTCPTCSGQGTVHMRQGFFSIQQTCPNCHGTGKIIPEPCPTCSGQGRIKKTKTL
                     EVKIPAGINEGMRIRSSGNGEPGPNGGPPGDLYIEIRIKQHDIFERDGDDLHCTVPVP
                     MTQAALGGTIEVPTLTGKVEIELPEGTQHGKTFRLRGKGIKGIRSSYPGDLYCHVTIE
                     TPIKLTEHQRKLLKELDESLRKGGDKHSPNSKSWTDRVKDLFK"
     gene            8615..10390
                     /locus_tag="VIN75_01425"
     CDS             8615..10390
                     /locus_tag="VIN75_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8972847.1"
                     /translation="MKPGLPAALGAGAGGFDSGLVNCKALVIDSNPTARSIQAAQLRD
                     LGVGTIVQCGRVQDARRQLEAREFDIVLCEQDFHGGNYSGQDLLNDLRRAQLLPFSTV
                     FVMVTSEASYAAVAEAAESALDSYLLKPHTATALAERIQQARHRKRILKDIFIAIDEG
                     QFDLAAQLCVRRFEVKGAYWLYAARIGAELLLRVNKHDEARVLYEAVIATQALPWARL
                     GVARAQIESGAAPAGRRTLESLIADQPGFADAYDVMGRVQVDQGQFAEALATYRTATQ
                     LTPGSISRLQRQGMLAFYLGENDEATKALERATLMGISSKMFDFQTIVLLAVTRFTAR
                     DSKGLQRCADNMKHALEKAAKSVRLQRFMGVITIFSLMLQRQVAQVVADIRAMAQDIT
                     QGDFDFEAASNMVAMLSYLTAAELQLDAAEGWIDTLALRFCTTRSLSELLARAGQAHE
                     PFANRIREQHNAITALAEQAVSHSLAGEPRVAAKSLLSAGARTMNAKLIDMARMVLAR
                     HADKIADAADLAGMADELRKRYGGSAAVAPLAADNGRQSGGLVLRARDKDSPGDVGAP
                     GTPDAPDAGVSAPGAEGADASGSAA"
     gene            10550..11647
                     /locus_tag="VIN75_01430"
     CDS             10550..11647
                     /locus_tag="VIN75_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY8972848.1"
                     /translation="MRSSETKRYIEIVPARGRGALSNRESRFMAAGREVDLAYLESIR
                     GELPETSIRTETTFVQARSIISRNDSPDIGFDRSINPYLGCEHGCVYCYARPTHAYLG
                     LSPGLDFETKLTAKANAAALLRKELSSRSYTPELIALGANTDPYQPIERDTRITRSVL
                     EVLSEFNHPVAITTKSAMVVRDIDILGPMAANGLARVAMSICTTDAEIARAMEPRANT
                     PARRFQAIEKLTRAGIPTSVIVAPIVPALTDAHLEQVIERAAEAGADSAHYVLLRLPL
                     EVRDLFVEWLETHFPLRAGHVMSLVNQMRGGKDYDSDFRTRMKGTGLLAELIAKRFEL
                     ACARHHLNRVRATLTTDLFAVPDASGQGTLF"
     gene            complement(11857..12258)
                     /locus_tag="VIN75_01435"
     CDS             complement(11857..12258)
                     /locus_tag="VIN75_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY8972849.1"
                     /translation="MNHTPSSPPAQVVATDATLALIHELQGRHGPLMFHQSGGCCDGS
                     APMCFAQGDFQLGDNDVHLGDIGGVPFYISESQYEYWCHTQLIIDVVPGRGGMFSLEG
                     PTGRRFLTRSRFYEDAEWRALVESGKARDVI"
     gene            complement(12354..>12902)
                     /gene="adh"
                     /locus_tag="VIN75_01440"
     CDS             complement(12354..>12902)
                     /gene="adh"
                     /locus_tag="VIN75_01440"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8972850.1"
                     /translation="VAAIKQGNPLDKSTMIGAQASSEQLEKILSYLDLGKQEGAKCLI
                     GGERNRLGEDLEGGFYVKPTVFAGHNKMRIFQEEIFGPVVSVTRFKDEEEALAIANDT
                     LYGLGAGVWSRDMNTAFRMGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMM
                     LDHYQQTKNLLVSYSDKALGFF"
BASE COUNT         2383 a         4508 c         4250 g         1761 t
ORIGIN      
        1 tcgatgctca acgacatggg catcgccgtc tacgagcagg cgcccgacgc cgccacgctg
       61 ctgatctcgg gccagaccca gtccggcgcg acggaagagg aagcggaaga agcggccgag
      121 gccgcgctgt cgaccgtcga ctccgaattc ggccgcacca ccgaccccgt gcgcatgtac
      181 atgcgcgaga tgggctcggt cgagctgctc acgcgcgaag gcgagatcga gatcgccaag
      241 cgcatcgaag gcggcctgca ggccatgatg caggcgatct ccgcgtcgcc cacgacgatc
      301 gccgagatcc tgctggtggc cgagcgcatc ggcaagggcg agatgcagat ctccgaagcc
      361 gtcgacggct tcgtctcgaa cgacgaggcg gacgactacg tcgccgagga agacttcgac
      421 gagttcgacg aggaagacga cgacgacggc gccggcggct ccaaggcgct gaccaagaag
      481 ctcgaggaac tcaaggccca ggccctgacc aagttcggcg agctcagcgc gcacttcgac
      541 aagatgcgca aggccttcga gaaggacggc taccgctcgc cgtcgtacga caaggcccag
      601 gaggccctga cgggcgacct gatgaagatc cgcttcacgg tcaagaccat cgagcgcctg
      661 tgctcgatcc tgcgcggcca ggtggacgac gtgcgccgct acgagcgcga gatccgcaag
      721 atcgtcgtcg acaagtgcgg catgccgcag gagcacttca tcaagacgtt cccggcgaac
      781 tcgctcaacc tgcactgggc cgagaaggag atcgccggca accgcgcgta cagcgtggtg
      841 atgagccgca acctgccggc catccaggaa ctgcagcaga agctgatcga cctgcaggcg
      901 cgcgccgtgg tgcccatcga gcacctgaag gacatcaacc gcaagatgaa cgagggcgag
      961 aaggcctcgc gcgacgccaa gaaggagatg atcgaggcca atctgcgcct cgtgatctcc
     1021 atcgccaaga agtacaccaa ccgcggcctg cagttcctcg acctgatcca ggaaggcaac
     1081 atcggcctga tgaaggcggt ggacaagttc gaatatcgcc gcggctacaa gttctccacg
     1141 tacgccacgt ggtggatccg ccaggccatc acgcgctcga tcgccgacca ggcccgcacg
     1201 atccgcatcc cggtgcacat gatcgagacg atcaacaaga tgaaccgcat ctcgcgccag
     1261 cacctgcagg agttcggctt cgagccggac gcgcccacgc tggccgagaa gatggagatg
     1321 cccgaggaca agatccgcaa gatcatgaag atcgccaagg agccgatctc catggagacg
     1381 ccgatcggcg acgacgacga ctcccacctg ggcgacttca tcgaggacac caacaacctc
     1441 gcgccggtgg aagccgcgat gcacgccggc ctgcgcgacg tggtcaagga catcctcgac
     1501 agcctgacgc cgcgcgaggc caaggtgctg cgcatgcgct tcggcatcga gatgtccacc
     1561 gaccacacgc tcgaggaagt gggcaagcag ttcgacgtca cgcgcgaacg catccgccag
     1621 atcgaagcca aggcgatccg caagctcaag cacccgagcc gctcggacaa gctgcggacc
     1681 tacctggaca acctctgaag agatgactgc ccccaggtcg tttcactccc ggcccccgag
     1741 gggcgctcag gccgcttcgg gcggccgggc gccggcctgg gaaatgactg cccccaggtc
     1801 gtttcactcc cggcccccga ggggcgctca ggccgcttcg ggcggccggg cgccggcctg
     1861 agataggagc taggcccgca tgtcagccat ccagcgtcgt gccgtcgtct tcgccgatct
     1921 tcgcggcagc accgcgctgt acgagttgct cggcaacacc gacgcgtccc agatcgtcac
     1981 cgagacggtg gacgcgatct cgcggcgcgt gcccgccacc ggcggcgtcg tcgtcaagac
     2041 gctcggcgac ggcctgatgg ccgtgttccc gacggccgcg caggccgtcg aggcggccga
     2101 ccagatgcac gaggagatcg acatcgtgat gcggcggcgc gacgcgctga ccggcttcac
     2161 ggaggcgcgt ctccagttgc aggtctgcat cgcgctgggc gagacggtgg aggtcagcgg
     2221 cgactacttc ggcgacgccg tcaacgtggc ggcgcgcctg ctcgaccacg ccggcgacaa
     2281 cgagacgctg gtcaccgccg acgtgctgcg cgccctgccc ctggactcgc gcgacatgtt
     2341 ccgcagcctc gacctcgtgc gcctgcgcgg gcgcgtcgag cccgtcgagg tgcacctgct
     2401 ttcgcgccgc ggcatggaca cggccccgac gctcgtgggc ggcggcccgc tgctgggcat
     2461 ggccggcccc gatgccgtca tcctcacctg gggcaacaag gtgcgccgct tcacggccac
     2521 cgacatgccc gtggtcatcg gccgcgccgc ggcttcggac atgcaggtgg acgatgcgcg
     2581 cgtctcgcgc tcgcactgcc gcatcgacct gatcggcgcc acgctgcagc tgtccgacct
     2641 cagcatcaac ggcaccttcg tgcgcttcac cagcgaggac gaggtgctga gcctgcgccg
     2701 cggcagctgc acgctgcatg gcagcggcga agtgggcctg agcggctcgc cggaggatcc
     2761 cggcgtcgcc gcgatgcagt tcgaggtcct gcacgcgccg ctgcggcgcg acgacgagta
     2821 cgacctcctg tcgcccatca tcgcgccgcc ctcgcgcaga tgagcgccgc tccgcgcgcg
     2881 cacgcgcacg acggcggcga caaggtcggc gtcctgctcg tcaacctcgg cacgcccgag
     2941 gcgccgacca cgccggccct gcgccgctac ctcgcgcaat tcctgtccga cccgcgcgtg
     3001 gtggagatcc cgcgcgcgct ctggtggctg atcctgcacg ccgtcatcct gcgcgtgcgg
     3061 ccgcgcagct cggccgccaa gtaccgcaag atctggctgc gcgaaggctc gccgctggcg
     3121 gtatggaccg cgcgccaggc caacatgctg cgcagctacg tcggcgagcg cggcgcgctg
     3181 gtcgaggtgc gctgggcgat gcgctacggc cagccggcga tcgccgccga gctcgacgcg
     3241 ctgtgcgccg ccggctgcac gcgcatcctc gtgctgccgg cctatccgca gtattcggcc
     3301 gccaccacgg ccagcgcgct cgacgacgtc ggcgcgtgga tgcggggcac gcgccgcctg
     3361 cccgagctgc gcttcgtcaa ccactaccac gacgaccccg gctacatcga ggcgctcgcc
     3421 gcccaggtgc gcaagcactg gcagcgcgag ggccgcggcc gcaagctggt gatgagcttc
     3481 cacggcatgc ccgcgcgcac gctgcagctg ggcgacccgt accactgcga atgcctgaag
     3541 acggcgcgcc tgctggccga gaagctccag ctggacgacg acgcctggcg cgtcaccttc
     3601 cagagccgct tcggccgcgc cgaatggctg cagccctaca ccgagccgac gctgcgcgcg
     3661 ctcgccaagg caggcgccga gggcgtggac gtgatctgcc ccggtttcgc ggccgactgt
     3721 ctcgagacgc tggaggagat cgcgatcgag gccaaggcgg ccttcctggc cgccggcggc
     3781 aagaccttcc actacatccc ctgcctgaac aacagccccg agggcatgaa ggcgatggcc
     3841 ggcgtggcgc tgcgccactt gcagggatgg gacaccaccc ccatgtcgac ggaagccctc
     3901 cagcaacgcc gcgcccgcgc gatcgcaagc ggcgcttccg aatagccgat gtcccgccat
     3961 accgcccctt ccgaagcccc tgccgccgcc gccgacatgc ggctcgacaa gtggctctgg
     4021 gtcgcgcgct tctacaagac gcgcggcctg gccgccgagg aagtcgagaa gggccgcgtg
     4081 caggtcaacg gccaggccgc caagccggcg cggtcggtgc gggtcaacga ccgcatcgag
     4141 atccgccagc ccggcacggt ccgtgaattg ctggtcctgg gcctgggcgc gatgcggggg
     4201 ccggcgacgg tggcccgcac gctgtaccag gagaccgaag ccagcatccg ggccgcgcag
     4261 caggccgcgg aggcgcgcaa gttcggcgtc gaaccagcgc atgcgcagga aaccggacgc
     4321 ccgaccaagc gcgaccgccg cgacctcgtc gactggcagc gctggagcgc ctcggtcgac
     4381 gacgaccggt agctgcgacg ccgcccggcc ggtgtccgcg gcaaaaaaag tttcgaacgg
     4441 cctcttgaaa ccctggaagc cgtctccatg tcaccgcgca acgcgaccgt aaaaccctga
     4501 cgacaacatg aatacggaag accagacccc cccaagccag cagcaaccgc cggcggacga
     4561 cggcgccgcc cacctcgaca tcggccttgg cggcgacgcc gcgcgcatcg ccgaactcga
     4621 agccaaggtg gccgaactga ccgacgccag tctgcgcgcg caggccgagg cgcagaatgc
     4681 ccggcgccgc gccgacgagg aagtggccaa ggcgcgcaag ttcgccgtcg agggcttcgc
     4741 cgacagcctg ctgcccgtca aggacagcct ggaggcggcc atcgccaccc ccggcgccgc
     4801 ggtcgagcag gtgctcgagg gcgtgcacgc cacgctgcgc cagctgtcgg ccgcgctgga
     4861 gcgcaacaag gtcgcgcaga tcaacccgcc cgcgggcact aagttcgacc cgcaccagca
     4921 ccaggccatc tcgatggtgc cggccgagca ggaagccaac acggtcgtga cggtgctgca
     4981 gaagggctac ctgatcgccg accgcgtgct gcgtccggcg ctggtcaccg tctcggcgcc
     5041 caagtgacgc ccggcgcggc ccgcgatgtc cctttcgagc tcgaaacgcc cttgaacgcg
     5101 catttcgccc ttgaataaga ccgcctcagc cgcatctcga aaacaacacg aatttcagct
     5161 tcaaccgcat cgcaggagaa accgacatgg gacgaatcat cggcattgac ctgggcacca
     5221 cgaactcgtg cgtgtccatc atggaaggca actcgaccaa ggtgatcgag aactcggaag
     5281 gcgcccgcac gacgccgtcc atcatcgcct accaggaaga cggcgaggtg ctggtcggcg
     5341 cgtccgccaa gcgccaggcc gtcaccaatc cgaagaacac cctgtacgcg gtcaagcgcc
     5401 tgatcggtcg caagttcacc gagaaggaag tccagaagga catcgacctg atgccctaca
     5461 agatcaccgc ggccgacaac ggcgacgcgt gggtcgaggt gcgcggcaag aagctcgcgc
     5521 cgcagcaggt cagcgccgaa gtgctgcgca agatgaagaa gacggccgag gattacctcg
     5581 gcgaggaagt gaccgaagcc gtcatcacgg tgccggccta cttcaatgac agccagcgcc
     5641 aggccaccaa ggacgccggc cgcatcgcgg gcctggacgt caagcgcatc atcaacgagc
     5701 cgaccgccgc ggcgctcgcc ttcggcctgg acaagcaggg caagggcgac cgcaagatcg
     5761 cggtgtacga cctgggcggc ggcacgttcg acatctcgat catcgagatc gccgatgtcg
     5821 acggcgagat gcagttcgag gtgctgtcca ccaacggcga caccttcctg ggcggcgagg
     5881 acttcgacca gcgcatcatc gacttcatcg tctcggagtt caagaaggaa cagggcgtcg
     5941 acctgaccaa ggacgtgctg gcgctgcagc gcctgaagga agccgcggag aaggccaaga
     6001 tcgagctgtc gaaccagacg cagaccgacg tcaacctgcc ctacatcacg gccgacgcct
     6061 cgggcccgaa gcacctgaac atcaagctga cgcgcgccaa gctcgagtcg ctggtggagg
     6121 atctgatcga gcgcacgatc gcgccgtgcc gcaccgccat caacgacgcc ggcgtgaaga
     6181 cgggcgacat caacgacgtc atcctggtcg gcggcatgac ccgcatgccc aaggtgcagg
     6241 acaaggtcaa ggagttcttc ggcaaggatc cgcgcaagga cgtcaacccg gacgaggccg
     6301 tggccgtcgg cgccgccatc cagggccagg tgctctcggg cgaccgcaag gacgtgctgc
     6361 tgctggacgt caccccgctg tcgctcggca tcgagacgct cggcggcgtg atgaccaaga
     6421 tgatcaagaa gaacaccacg atcccgacca agttcagcca ggtgttctcg accgccgacg
     6481 acaaccagcc ggccgtcacg atcaaggtct accagggcga gcgcgagctg gcctcgggca
     6541 acaagagcct gggcgagttc aacctcgagg gcatcccgcc ggcgccccgc ggcacgccgc
     6601 agatcgaggt cacgttcgac atcgacgcca acggcatcct gcacgtgggc gccaaggaca
     6661 aggccaccgg caaggaaaac aagatcacca tcaaggcgaa ctcgggcctg agcgaggacg
     6721 agatccagaa gatggtgcgc gacgcggaaa tcaacgccgc cgaggacgcc aagaaggtcg
     6781 agctcgtgtc ggcgcgcaac cagggcgacg ccatggtgca ctcggtgcgc aagagcctgg
     6841 gcgagcacgg cgacaagctc gaagcctccg agaagcaagc catcgagacc gcgctgaagg
     6901 acgtcgagga agccatcaag ggcgacgaca aggccgcgat cgaagccaag acggaagccc
     6961 tgatgaccgc cagccagaag ctgggcgaga aggtctacgc cgaccagcag gccgcccagg
     7021 cggcagcggg cgcggcggcg ggcggcggcg cccaggcgca ggccgaaccg caggcggcgc
     7081 acgcggccga cgacaacgtg gtcgacgccg acttcaagga agtcaagaag agctgaggcg
     7141 tcgcgcctgg ctccggccgg tgacttgcgg ggactcgacg gtcccgcggg ccgccggcct
     7201 ttctcgctga tagacagacg cgatccggat gcgatcctgc acgcatccgg cgctgcccct
     7261 gcgcggattc ccggcgcgct gaagagacaa ttgccccatg agcaaacgcg attactacga
     7321 agtccttggc ttgacgaagg gtgcgtcgga cgacgacatc aagaaggcct atcgcaagct
     7381 cgcgatgaag caccacccgg accgcaacca gggtgacggc gccaaggcgg ccgaggagaa
     7441 gttcaaggag gccaaggagg cctacgagat gctctccgac ccgcagaaga aggccgccta
     7501 cgaccagtac ggccatgcgg gcgtggaccc gaacatgggc cacggcgccg gcgagggctt
     7561 cggcggcttt gccgaggcgt tcggcgacat cttcggcgac atcttcggcg gcggccgtcg
     7621 cccgggcggc ggtggcggca agcaggtcta ccgcggcaac gacctcagct acgcgatgga
     7681 gatcacgctg gaggaggccg cggccggcaa ggacacgcag atccgcgtgc ccagctggga
     7741 gaactgcgag acctgcaagg gctcgggcgc caagcccggc acctcgccca agacctgccc
     7801 cacctgctcg ggccagggca cggtgcacat gcgccagggc ttcttctcga tccagcagac
     7861 ctgccccaac tgccacggca cgggcaagat cattcccgag ccctgcccca cctgctcggg
     7921 ccagggccgc atcaagaaga ccaagacgct ggaagtcaag atcccggcgg gcatcaacga
     7981 aggcatgcgc atccgctcgt cgggcaacgg cgagccgggc cccaacggcg gcccgccggg
     8041 cgatctgtac atcgagatcc gcatcaagca gcacgacatc ttcgagcgcg acggcgacga
     8101 cctgcactgc acggtgccgg tgcccatgac gcaggccgcg ctcggcggca cgatcgaggt
     8161 tcccacgctc accggcaagg tcgagatcga gctgcccgaa ggcacgcagc acggcaagac
     8221 cttccgcctg cgcggcaagg gcatcaaggg catccgctcg agctaccccg gcgacctgta
     8281 ctgccacgtc accatcgaga cgccgatcaa gctcaccgag caccagcgca agctgctcaa
     8341 ggagctcgac gagtccctgc gcaagggcgg cgacaagcat tcgcccaatt ccaagagctg
     8401 gacggaccgg gtcaaggacc tgttcaagta agccagccga cgcgcgaaac gcgcaaatcg
     8461 aaagccgccc ctcgaggcgg cttttcttcg tccgggtttg tcccgcggcg cgccccatca
     8521 agttctcccg acacccggcc gataccccat ggacggacgt gcatccgccc gtccgggcga
     8581 gggtgttcgc gtcatgaaga gtgtgcagtc ggagatgaaa ccaggcctgc cggccgccct
     8641 gggcgccggt gcgggtggct tcgactcggg cctcgtcaac tgcaaggcgc tggtgatcga
     8701 ctccaacccg accgcccgca gcatccaggc cgcgcagctg cgcgacctcg gcgtgggcac
     8761 catcgtgcag tgcggccgcg tgcaggacgc ccgccgccag ctcgaggcgc gcgagttcga
     8821 catcgtgctg tgcgagcagg acttccacgg cggcaactac tccggccagg acctgctcaa
     8881 cgacctgcgc cgcgcgcagt tgctgccgtt ctccaccgtc ttcgtgatgg tgacctcgga
     8941 ggcgagctac gccgccgtcg cggaggccgc ggaatcggcg ctggacagct acctgctcaa
     9001 gccgcacacc gcgacggcgc tggccgagcg catccagcag gcgcgccatc gcaagaggat
     9061 cctcaaggac atcttcatcg ccatcgacga gggccagttc gacctcgccg cgcagctgtg
     9121 cgtgcgccgc ttcgaggtca agggcgccta ctggctgtac gccgcgcgca tcggcgccga
     9181 gctgctgctg cgcgtgaaca agcacgacga ggcacgcgtg ctgtacgagg ccgtcatcgc
     9241 cacgcaggcg ctgccgtggg cgcgactggg cgtggcgcgg gcgcagatcg aatcgggcgc
     9301 ggccccggcc ggtcgccgca ccctcgagag cctgatcgcc gaccagcccg gcttcgccga
     9361 cgcctacgac gtgatgggcc gcgtgcaggt cgaccagggc cagttcgccg aggcgctggc
     9421 cacgtaccgc acggccacgc agctcacgcc gggctcgatc agccgcctgc agcggcaagg
     9481 catgctggcg ttctacctcg gcgagaacga cgaggcgacc aaggcgctcg aacgcgcgac
     9541 gctgatgggc atcagctcga agatgttcga cttccagacc atcgtgctgc tggccgtcac
     9601 ccgattcacc gcgcgcgatt ccaagggcct gcagcgctgc gcggacaaca tgaagcacgc
     9661 gctcgagaag gcggcaaaga gcgtgcgcct gcagcgcttc atgggcgtca tcaccatctt
     9721 cagcctgatg ctgcagcgcc aggtggccca ggtggtggcc gacatccgcg ccatggcgca
     9781 ggacatcacg caaggcgact tcgacttcga agccgcgtcc aacatggtgg cgatgctgtc
     9841 gtacctcacg gccgcggagc tgcagctgga cgccgccgaa ggctggatcg acacgctggc
     9901 gctgcgcttc tgcacgacgc gcagcctgtc ggagctgctg gcgcgcgcgg gccaggcgca
     9961 cgagccgttc gccaaccgca tccgcgagca gcacaacgcc atcaccgcgc tggccgagca
    10021 ggccgtctcg cacagcctgg ccggcgagcc gcgcgtggcc gcgaaatcgc tgctcagcgc
    10081 cggcgcgcgc acgatgaacg ccaagttgat cgacatggcg cgcatggtgc tggcgcgcca
    10141 cgccgacaag atcgcggacg ccgccgacct ggcgggcatg gccgacgagc tgaggaagcg
    10201 ctatggcggc agcgccgccg tggccccgct ggcggccgac aacggacggc aatccggcgg
    10261 cctggtgctg cgggcgcgcg acaaggactc ccccggcgac gttggcgcac caggcacccc
    10321 ggacgccccg gacgccggcg tctccgcacc gggcgcagaa ggcgcggacg cgtctggcag
    10381 cgccgcatag aacggccagg ggcggcgcac gacggccgcg ggccggactt cgcaggattg
    10441 attcaagaca ggcctttctc cacgcaagcc gcggcgggcc catggacgac attccggggg
    10501 caggcacgcg cgaccttgac tgtatgttta tacagtatag actgagccga tgcgatcttc
    10561 cgagaccaag cgctacatcg agattgttcc ggcccgggga cggggcgccc tgtcgaacag
    10621 ggaatcgcgc ttcatggccg ccggccgcga ggtggatctc gcctatctcg agtcgatccg
    10681 tggagaactt ccagagactt ccattcgcac cgagacgaca ttcgttcaag ccaggtcgat
    10741 catcagcagg aacgactcgc cggacatcgg gttcgatcgc tcgatcaatc cttacctggg
    10801 ctgcgagcac ggatgtgtct actgctacgc gcgtccgacc catgcgtacc tgggcctgtc
    10861 gccgggcctc gatttcgaga ccaagctcac ggccaaggcg aacgcagccg ccctcctccg
    10921 caaggaactg tcttcccgtt cctatacgcc ggaactcatc gccctcgggg ccaacacgga
    10981 cccttaccag cccatcgaaa gggacactcg gatcacgcgc agcgtcctcg aggtcctgtc
    11041 ggagttcaat cacccggtcg ccatcaccac gaaatcagcg atggtcgtgc gcgacatcga
    11101 cattctcggc cccatggccg ccaacggcct cgcacgcgtc gccatgtcga tctgcacgac
    11161 cgacgcggag atcgcacgcg cgatggaacc cagggcgaac acccctgccc ggcggttcca
    11221 ggcgatcgag aaactcactc gcgccggcat cccgacgtca gtgatcgtgg cgcccattgt
    11281 tcccgcgttg acggacgcgc atctggaaca agtcatcgag cgtgcagccg aggccggcgc
    11341 cgattcggcc cactacgtgt tgctcaggct tccactcgag gtccgcgacc tgttcgtcga
    11401 atggctcgag acgcactttc cgctccgcgc cggacatgtg atgagcctgg tcaaccagat
    11461 gcgcggaggg aaggactacg actcggactt ccgcacgcgc atgaaaggca ccggcctcct
    11521 tgccgaattg atcgcgaagc gctttgaact cgcctgtgcc aggcaccacc tgaaccgcgt
    11581 tcgcgcgacg ctcacgacgg acttgttcgc ggttccggac gccagcgggc aaggcactct
    11641 gttctagccg gctatccgcc catccttgag cggagtggac cgatcgggac tctcgcatgt
    11701 ttcggtccga agcctctgcg cgatcgcgca gccgctttgc agcaacagcc ggcctgcgcg
    11761 tcgacgggag ttccgtccgc ttgcggccgc tcggctgacg tcaggtgacg accaatggca
    11821 tccgccgccg gcgcgacgcg ttcgcaccag cgccgctcag atgacatctc tggcctttcc
    11881 gctctcgacg agcgcacgcc attccgcgtc ttcgtagaag cgggagcggg tcaggaagcg
    11941 ccgtcccgtc gggccttcca gagagaacat gccgccgcgt cccgggacga cgtcgatgat
    12001 cagctgggtg tgacaccagt actcgtattg cgactcgctg atgtagaacg gcacgccgcc
    12061 gatgtcgccc aggtggacgt cgttgtcgcc cagctggaag tcgccctgcg cgaagcacat
    12121 cggggcgctg ccgtcgcagc agccgccgga ctggtggaac atcagcgggc cgtggcgacc
    12181 ctgcaattcg tggatcagcg ccagcgtcgc atcggtcgcg acgacctggg ccggaggact
    12241 cgacggggtg tggttcatcg cgcgcgcctt ggatgggaga catcccgcgg accacgcacg
    12301 gcctcgtcca cgggatgtcg attccggcgg gagcgccggc gggaacggac ggctcagaag
    12361 aagccgagcg ccttgtcgct gtagctcacg agcaggttct tggtctgctg gtagtggtcg
    12421 agcatcatct tgtggttctc gcggccgatg cccgactgct tgtagccgcc gaacgccgca
    12481 tgcgccgggt aggcgtggta gcagttcgtc cacacgcggc cggcctggat gccgcggccc
    12541 atgcggaagg cggtgttcat gtcgcgcgac cagacaccgg cgcccagccc gtacagcgtg
    12601 tcgttggcga tggccagcgc ctcttcctcg tccttgaacc ttgtcaccga caccacgggg
    12661 ccgaagatct cctcctggaa gatgcgcatc ttgttgtggc ccgcgaagac ggtgggcttg
    12721 acgtagaagc cgccctccag gtcctcgccc agccggttgc gctcgccgcc gatcaggcac
    12781 ttggcgcctt cctgcttgcc caggtcgagg tagctcagga tcttctcgag ctgctcgctg
    12841 gatgcctgcg cgccgatcat ggtcgacttg tccagcgggt tgccctgctt gatggcggcc
    12901 ac
//
LOCUS       DATCRO010000054         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_585594, whole genome shotgun sequence.
ACCESSION   DATCRO010000054 DATCRO010000000
VERSION     DATCRO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585594"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VIN75_01445"
     CDS             complement(<1..725)
                     /locus_tag="VIN75_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8972851.1"
                     /translation="MTTSVSFASASDTREQKPRLQELGPGAYGYISDFDPNCGFVVGD
                     EHVVLIDTRPTPRMARDFLAAIRTVTDKPIRTIVLTHYHAVRVMGASAFAEVRDIVAS
                     SGTLYWIRTRGQADFDSEVGRFPRLFAGVEEIPGLTMPTKVFDREMTLPLGGGRELRL
                     MSLGRGHSGGDAVAWLADCGVLFSGDVVENRCGVYAGDAYIGDWAGTLDAVAALKPRV
                     LVPGRGAVLQDEAACAEAISLTKA"
     gene            complement(722..934)
                     /locus_tag="VIN75_01450"
     CDS             complement(722..934)
                     /locus_tag="VIN75_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEY8972852.1"
                     /translation="MITEPRIPDPDGFYAALLAAHEGRSEAQSADLNARLVLLLANQC
                     ADQDVLLACIRAAAEEEPTTTDHDTP"
     gene            complement(931..2583)
                     /locus_tag="VIN75_01455"
     CDS             complement(931..2583)
                     /locus_tag="VIN75_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8972853.1"
                     /translation="MEKFAFAPAYELPVWPFTPPPELGSTKPVRHPIVIVGAGPSGLT
                     LACDLAQRGVRAVLLDEDDTVGVRGASSRGICYAQKSLEIFERLGIYERIAAKGITWS
                     FGRTFSGEQEVYNFNLQANSVSRQPPFINLQQFYVEWFLVDRILELGLTDLRWKNRVT
                     RVERLADGVRLEVETPAGSYTIEADRLIDATGANSAIRTQMGIDAHSSRSTDRWCISD
                     VRFRKPLPTERWTWVDAPFNEGRGVWQHLMADGVWRIDYQMPEDCDTAYISKPEVAGA
                     RLREQLGPDVEFEFVWIGPYGYRDHLLDSFRHGRVFFIGDAAHVVSPFGARGGNSGIQ
                     DAANLGWKLALVAQGRAGEALLDSYDAERQPAAKQNLQVTSRSARFLAPRSPAEHTLR
                     RAVVALAARHPFARALVNTGRMSVANDYPPAPLLPEGARSVQNLPLRLADGRETTLMQ
                     LLAEGTECIGLWFAPTREQSRAALDAIASLPLRLLAVGGDSGLPTLQADEKLASHLGA
                     GDAGSFVLVRPDAYRAAVLPNATPEAIARAVRTALAANELDP"
     gene            complement(2597..>2907)
                     /locus_tag="VIN75_01460"
     CDS             complement(2597..>2907)
                     /locus_tag="VIN75_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8972854.1"
                     /translation="RQVNYSANAMGRTTSLTAFLSQWSSRSQADGPTNLARTTVPKLL
                     LTYTGDQSTFPSTRDAWMAAGGPRIRNVDIVGGNHYLAGQPELIPKAADAIAGFAHAL
                     "
BASE COUNT          396 a          980 c         1092 g          439 t
ORIGIN      
        1 gccttggtga ggctgatggc ctcggcgcag gcggcctcgt cctgcagcac cgcgccgcgg
       61 ccaggcacca gcacgcgcgg cttcagcgcg gccacggcgt cgagcgtgcc ggcccagtcg
      121 ccgatgtagg cgtcgcccgc gtacacgccg cagcggttct cgaccacgtc gcccgagaag
      181 agcacgccgc agtcggcgag ccacgcgacc gcgtcgccgc ccgaatggcc gcggcccagc
      241 gacatcagcc gcagttcgcg gccgccgccc agcggcagcg tcatctcgcg gtcgaagacc
      301 ttcgtgggca tggtcaggcc cggtatctcc tcgacgccgg cgaacagccg cggaaagcgg
      361 ccgacctccg agtcgaagtc ggcctggccg cgcgtgcgga tccagtagag cgtgccgctg
      421 ctggcgacga tgtcgcgcac ctcggcgaag gcgctggcgc ccatcacccg caccgcgtgg
      481 tagtgcgtga gcacgatggt gcggatcggc ttgtcggtca ccgtgcggat ggcagcgagg
      541 aagtcgcgcg ccatgcgcgg cgtggggcgg gtgtcgatca gcaccacgtg ctcgtcgccg
      601 acgacgaagc cgcagttggg atcgaagtcg ctgatgtagc cgtaggcgcc ggggcccagc
      661 tcctgcagcc ggggcttctg ctcgcgggtg tcggaggcgg atgcgaagga aacgctcgtg
      721 gtcatggggt gtcgtggtcc gtcgtcgtgg gttcttcttc ggccgccgcg cggatgcacg
      781 cgagcagcac gtcctggtcg gcgcactggt tggccagcag cagcacgagg cgcgcgttga
      841 ggtcggcgct ctgcgcctcg ctgcggcctt cgtgggcggc gagcagcgcg gcgtagaagc
      901 cgtcgggatc ggggatgcgg ggctcggtga tcatgggtcg agttcgttgg cggccagtgc
      961 ggtgcgcacg gcgcgggcga tggcttcggg cgtggcgttc ggcagcacgg cggcgcggta
     1021 ggcgtcgggc cgcacgagga cgaagctgcc tgcgtcgccc gcgccgagat ggctcgcgag
     1081 cttttcgtcg gcctgcagcg tgggcaggcc gctgtcgccg ccaacggcca gcaggcgcaa
     1141 cggcagcgac gcgatggcat cgagcgccgc tctcgactgc tcgcgcgtcg gcgcgaacca
     1201 caggccgatg cactcggtgc cttcggccag cagctgcatc agcgtcgtct cgcggccatc
     1261 ggccaggcgc agcggaaggt tctgcaccga gcgcgcgccc tcgggcagca gcggcgcggg
     1321 cggatagtcg ttggccaccg acatccggcc ggtgttgacc agcgcccgcg cgaagggatg
     1381 gcgcgccgcc agcgccacca ccgcacggcg cagcgtgtgc tcggcgggcg agcgcggcgc
     1441 gaggaagcgt gccgatcggc tcgtcacctg caggttctgc ttcgcggcgg gctggcgctc
     1501 ggcgtcgtag ctgtcgagca gcgcctcgcc ggccctgccc tgcgcgacga gcgcgagctt
     1561 ccagccgagg ttggccgcgt cctggatgcc gctgttgccg ccgcgtgcgc cgaaggggct
     1621 caccacgtgc gccgcgtcgc cgatgaagaa cacgcggccg tggcggaagc tgtcgagcag
     1681 gtgatcgcgg tagccatagg ggccgatcca gacgaactcg aactccacgt ccggcccgag
     1741 ctgctcgcgc agccgcgcgc ccgccacctc gggcttgctg atgtaggcgg tgtcgcagtc
     1801 ctcgggcatc tggtagtcga tgcgccacac gccatcggcc atcaggtgct gccacacgcc
     1861 gcggccttcg ttgaagggcg cgtccaccca ggtccagcgc tcggtgggca gcggcttcct
     1921 gaagcgcacg tcgctgatgc accagcggtc ggtgctgcgc gaggagtgcg cgtcgatgcc
     1981 catctgcgtg cggatcgcgc tgttggcgcc ggtggcgtcg atcagccggt cggcctcgat
     2041 ggtgtagctg ccggcgggcg tctcgacttc caggcgcacg ccgtcggcga gccgctcgac
     2101 gcgtgtgacg cggttcttcc agcgcaggtc ggtcagcccc agctcgagga tgcgatcgac
     2161 caggaaccac tcgacgtaga actgctgcag gttgatgaaa ggcggctgcc gcgagacgct
     2221 gttggcctgc aggttgaagt tgtagacctc ctgctcgccc gagaaggtgc gcccgaacga
     2281 ccaggtgatg cccttggccg cgatgcgctc gtagatgccc aggcgctcga agatctccag
     2341 gctcttctgc gcatagcaga tgccgcgcga cgatgcgccg cgcacgccga cggtgtcgtc
     2401 ctcgtccagc agcacggcgc gcacgccgcg ttgcgcgagg tcgcaggcca gcgtgaggcc
     2461 cgaggggccg gcgccgacga tgacgatggg atgccgcacg ggcttcgtgc tgcccagctc
     2521 gggcggcggc gtgaagggcc agaccggcag ctcgtaggcc ggtgcgaaag cgaatttttc
     2581 catggtggct ctgcggctac agcgcgtgcg cgaacccggc gatggcgtcc gccgccttcg
     2641 ggatcagctc cggctgcccc gcgaggtagt ggttgccgcc gacgatgtcg acgttgcgga
     2701 tgcgcggccc gcccgccgcc atccaggcat ctcgcgtgct cgggaaggtc gactggtcgc
     2761 ccgtgtaggt cagcagaagt ttcggcaccg tggtgcgcgc gaggttggtc ggaccgtcgg
     2821 cctgcgagcg cgacgaccac tgcgagagaa aggccgtgag cgacgtggtg cgtcccatcg
     2881 cgttggccga gtagttcacc tgccgcg
//
LOCUS       DATCRO010000055         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_911983, whole genome shotgun sequence.
ACCESSION   DATCRO010000055 DATCRO010000000
VERSION     DATCRO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911983"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VIN75_01465"
     CDS             complement(<1..889)
                     /locus_tag="VIN75_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY8972855.1"
                     /translation="MQTALNPQSTPRAIPSTPSQPQRDTAGSPTGQNLAHYQIIRRNG
                     AVVPFEPNKIAIAMMKAFLAVHGTQGAASASVRETVDGLTQAVVRGLVRSRPGGGTFH
                     IEDVQDQVELGLMRGGHHEIARAYVLYRERRTQERSKQGEQTAPATPALHVLDKGELV
                     ALDVNELKSLIESACEGLGESITAAPIVAETMRNLYDGVPLDEVYKASILAARTLIEK
                     DPDYTFATARLLLHTIFKEIIGREVLPAERATAYADYFPQFIKKGVDNDLLDEKLLQY
                     DLPRLGAALKPERDNQFDYL"
     gene            complement(1240..1857)
                     /gene="ampD"
                     /locus_tag="VIN75_01470"
     CDS             complement(1240..1857)
                     /gene="ampD"
                     /locus_tag="VIN75_01470"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY8972856.1"
                     /translation="MPTTEEEARAAESGDALWQAGWYRFAKALPSPNFGPRPQGAQID
                     LIVLHSISLPPGEYGGDAVQRLFTNRLDWDAHPYFQSIRGLEVSSHFYVRRNGELWQF
                     VSCDDRAWHAGVSTWRGRGNCNDDSIGIELEGLEGQHFEEPQYETLASLCAAIAQHFP
                     VAYIAGHEHIAPGRKQDPGAGFDWRLLREQLGWKPQMFPEQVVGR"
     gene            complement(1844..>2641)
                     /locus_tag="VIN75_01475"
     CDS             complement(1844..>2641)
                     /locus_tag="VIN75_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8972857.1"
                     /translation="ARGGTLFLDEIGDLPLAMQSKLLRAIQERSVRSIGSTQEDAVDV
                     RIVSATHKDLHAEVQAGRFRQDLFYRLNVIEIAVPALRDRREDLPALCNALLSRIAQD
                     GGLPVPHLSDDLLRRLAQHPLNGNVRELENLLHRAVALNDGDELHLDLMGGFTAAAEA
                     AASSAPAPLSPLGGDAAAAPAPAPAAPVAASTRPAPPPLPSDLQAYLDQQEREILVRA
                     LHESGFNRTAAAARLGMSLRQIRYRIARLGITTPNGDDGASSSHADD"
BASE COUNT          375 a          839 c          969 g          458 t
ORIGIN      
        1 cgaggtagtc gaactggttg tcgcgctcgg gcttcagggc cgcgcccagg cggggcaggt
       61 cgtactgcag cagcttctcg tcgagcaggt cgttgtccac gcccttcttg atgaactgcg
      121 ggaagtagtc ggcgtaggcg gttgcgcgtt cggcgggcag cacttcgcgg ccgatgatct
      181 ccttgaagat cgtgtgcagc agcaggcgcg cggtggcgaa ggtgtagtcg gggtccttct
      241 cgatcagcgt gcgggccgcc aggatcgatg ccttgtagac ctcgtcgagc ggcacgccgt
      301 cgtacaggtt gcgcatcgtc tcggcgacga tcggcgcggc ggtgatgctc tcgcccaggc
      361 cttcgcaggc cgattcgatc aggctcttga gctcgttcac gtcgagcgcg accagctcgc
      421 ccttgtccag cacgtgcagc gcgggcgtgg ccggcgcggt ctgctcgccc tgcttggagc
      481 gttcctgcgt gcggcgctcg cggtacagca cgtaggcgcg ggcgatctca tggtggccgc
      541 cgcgcatcag gccgagctcg acctggtcct gcacgtcctc gatgtggaag gtgccgccgc
      601 ccgggcgcga gcgcaccagg ccgcgcacga cggcctgcgt gaggccgtcg accgtctcgc
      661 gcacgctggc cgacgcggcg ccctgcgtgc cgtgcacggc gaggaaggcc ttcatcatcg
      721 cgatggcgat cttgttcggc tcgaagggca ccaccgcgcc gttgcggcgg atgatctggt
      781 agtgggccag gttctggccg gtgggggagc cggcagtgtc tcgctgcggc tgcgacggcg
      841 tggaggggat tgcacgcggg gtcgattggg gattcagggc tgtttgcatt cgcttcctct
      901 cggttggcaa ttcttgttgg atggcgacgc ctggcccggc gccgcaccgg tgtacgggac
      961 cggacggaag acactatatc tagggtgcag ggggcataca acccactaga tgtagcgttt
     1021 tcgaaaggat acaaggattt ccggtactac ttccgacata ggacacctgc gaagatgccc
     1081 catgaatact ggcctatggt cccgcaaccc gcatgcgctg tggcttgcag gccgatttgg
     1141 gcttgcaggg cgcatggttg ctgcattgcg cccttcggat tttctcttcg ctgcagcgcg
     1201 gtggcggtgc atcgcgaggc gccgcgcgat tgaaaaaaat tagcgcccga cgacctgctc
     1261 aggaaacatc tgtggcttcc agccgagttg ttcgcgcaga aggcgccagt cgaaaccggc
     1321 gcccgggtcc tgcttgcggc ccggcgcgat gtgctcgtgg ccggcgatgt acgccaccgg
     1381 aaaatgctgc gcgatggcgg cgcacaggct cgcgagcgtc tcgtattgcg gctcctcgaa
     1441 atgctggcct tcgaggccct cgagttcgat gccgatggag tcgtcattgc agttgccgcg
     1501 gccgcgccac gtcgacacgc ccgcatgcca ggcgcggtcg tcgcagctca cgaactgcca
     1561 gagctcgccg ttgcgccgca cgtagaagtg cgaggagacc tccagcccgc ggatcgactg
     1621 gaagtagggg tgtgcgtccc agtcgagccg gttggtgaag agccgctgca ccgcgtcgcc
     1681 gccgtactcg ccgggcggca ggctgatcga gtgcagcacg atgaggtcga tctgcgcgcc
     1741 ctgtggcctg ggcccgaaat tgggcgaggg cagcgccttc gcgaagcggt accagccggc
     1801 ctgccagagt gcgtcgccgg actcggccgc gcgcgcttct tcttcagtcg tcggcatggc
     1861 tgctgctcgc gccgtcgtcg ccgttgggcg tggtgatgcc cagccgcgcg atgcgatagc
     1921 ggatctggcg caggctcatg cccagccggg cggcggcggc ggtgcggttg aagccgctct
     1981 cgtgcagcgc gcgcacgagg atctcgcgct cctgctggtc gaggtaggcc tgaagatcgg
     2041 agggcagcgg cggaggcgcg ggccgggtag aggccgccac cggcgcggcc ggcgccgggg
     2101 ccggagcggc cgccgcgtcg ccgcccaggg gcgaaagcgg cgcgggcgcg gaagatgccg
     2161 ccgcttcggc cgcggccgtg aagccgccca tcaggtcgag gtgcagttcg tcgccgtcgt
     2221 tcagcgcgac cgcgcggtgc agcaggttct cgagctcgcg cacgttgccg ttcagcggat
     2281 gctgtgcaag gcggcgcagc agatcgtcgg acaagtgagg caccggcagc ccgccgtcct
     2341 gcgcgatgcg cgagagcagc gcgttgcaca gcgcgggcag gtcctcgcgg cggtcgcgca
     2401 gcgcgggcac cgcgatctcg atcacgttga ggcggtagaa aaggtcttgc cggaagcggc
     2461 cggcctgcac ttcggcgtgc aggtctttat gagtggcgct cacgatgcgc acgtcgacgg
     2521 catcttcctg cgtcgagccg atcgagcgca cgctgcgctc ctggatcgcg cgcagcagct
     2581 tcgactgcat ggccagcggc aggtcgccga tctcgtcgag gaacagcgtg ccgccgcgcg
     2641 c
//
LOCUS       DATCRO010000056         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_315657, whole genome shotgun sequence.
ACCESSION   DATCRO010000056 DATCRO010000000
VERSION     DATCRO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315657"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VIN75_01480"
     CDS             <1..539
                     /locus_tag="VIN75_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858072.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HEY8972858.1"
                     /translation="FTAIAQARVPLGGGAAISVGALIALATFGAALYVAHGTAFGRNV
                     YAIGGSEHSAVLMGLPVGRTVVGVYALSGLCAALGGVLFTFYMLSGYGLHATGLELDA
                     IASVVIGGTLLAGGVGLVAGTLCGVLMLGLIQTLITFDGSLSAWWTKIVIGALLLAFC
                     LLQRLTQRGQPGAEPALA"
     gene            complement(542..970)
                     /locus_tag="VIN75_01485"
     CDS             complement(542..970)
                     /locus_tag="VIN75_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8972859.1"
                     /translation="MNYPLPRNHFIVQWGGTRIGFSEVGGLSIDAQAPAFRDGSSLAN
                     SNSTMPGLLRYPNLVLKRAVQKGDNEFYAWINTVHNSAVERRDITVSLLDADNQPVVI
                     WTFKNAFPARLVYSSLESENAGPMMEVLEIAHEGMRVQNA"
     gene            complement(1168..2226)
                     /locus_tag="VIN75_01490"
     CDS             complement(1168..2226)
                     /locus_tag="VIN75_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY8972860.1"
                     /translation="MNEEFQRRMEEAARLVRGGSLMDATAAIQQALGARPPAPRGGMA
                     VADEDVIDVQARVIDEPAVPPRTMPASRPESFCAGHFRNDCGTRDYKLFEPARPDPRP
                     LPLIVMLHGCTQDPDDFARGTRMNALARARGFLVLYPAQSQRANAQRCWNWFKHNHQA
                     RGKGEPGILAGMVRQVVVSHDIDPARVYVAGLSAGGAMAAILGDAYPDLFAAVGVHSG
                     LPAGSATDVQTAFAAMGGGPTAPRRSPKAGATPPTIVFHGDADATVNVVNGERVLAAA
                     GVSASDDVRHGVSAAGVPWTRRTWVGGQGAERAEQWTIHGAGHAWSGGDLSGTYTDPR
                     GPDASAEMLRFFLAHPRN"
     gene            complement(2317..>2826)
                     /locus_tag="VIN75_01495"
     CDS             complement(2317..>2826)
                     /locus_tag="VIN75_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8972861.1"
                     /translation="VSKKPRPAPQALVDAFRGFESGESMVDVRVGGWSELGAEAQAIR
                     TAVFVEEQKVPLALERDAADVSAVHALARNRLGLAVGTGRLLAADPAGHPARIGRMAV
                     NRAVRGANVGRAMLEALMAAAGQRGDPEVALHAQITAFGFYRRAGFRPHGERFEEAGI
                     VHQEMRRAP"
BASE COUNT          346 a         1032 c         1038 g          410 t
ORIGIN      
        1 ccttcacggc catcgcccag gcgcgcgtgc cgctgggcgg cggcgcggcg atctcggtcg
       61 gggccctgat cgcgctcgcc accttcggcg cggcactgta cgtcgcgcac ggcaccgcgt
      121 tcgggcgcaa cgtctacgcg atcggcggca gcgagcactc ggccgtgctg atgggcctgc
      181 ccgtcgggcg cacggtggtg ggcgtctacg cgctcagcgg gctgtgcgcc gcgctcggcg
      241 gggtgctgtt cacgttctac atgctctcgg gctacggcct gcatgcgacc ggcctggagc
      301 tcgatgccat cgcctcggtg gtgatcggcg gcacgctgct cgcgggcggc gtgggcctgg
      361 tggccggcac gctgtgcggc gtgctgatgc tggggctgat ccagacgctg atcaccttcg
      421 acggctcact gagcgcctgg tggacgaaga tcgtcatcgg cgccctgctg ctggcgttct
      481 gcctgctgca gcggttgacg cagcgcgggc agccgggggc ggagccggcg ctggcctgac
      541 gtcaggcgtt ctgcaccctc atgccctcgt gggcgatctc tagcacttcc atcatcgggc
      601 ccgcgttctc cgattcgagc gacgagtaca cgagccgggc gggaaacgcg ttcttgaacg
      661 tccagatcac gaccggctgg ttgtccgcat cgagcagcga cacggtgatg tcgcggcgct
      721 cgaccgcgct gttgtggacc gtgttgatcc acgcgtagaa ctcgttgtcg cctttctgca
      781 ccgcgcgctt gagcaccagg ttcggatagc gcagcagccc cggcatcgtg gagttgctgt
      841 tggccagcga cgagccgtcg cggaacgccg gcgcctgggc gtcgatggac aggccgccca
      901 cctcgctgaa gccgatgcgc gtgccgcccc actggacgat gaagtgattc ctgggcaacg
      961 gatagttcat gggcggctcc tgcggcaggg gcgcgaggac ggcgtgatgc ggcgcgccgg
     1021 ggcctcgcac cccggcgcct gaaattctgc agccgatggc gcgggtcgac acggcgatgg
     1081 cgccgcagcc cccccaattc gacgagcact tctgcgcagg gggcggccgg caggcatgtg
     1141 gcgccgccag gtggacgccg gcagccgtca gttccgggga tgcgccagga agaaccgcag
     1201 catctccgcg ctcgcgtccg gcccgcgcgg gtcggtgtac gtgccggaca ggtcgccgcc
     1261 cgaccaggcg tggccggcgc cgtggatcgt ccattgctcg gcgcgctcgg cgccctgccc
     1321 gccgacccag gtgcggcgtg tccagggcac gcccgcggcg ctcacgccgt gccgcacgtc
     1381 gtcgctcgcg ctgacgcccg ccgccgccag cacgcgttcg ccgttgacga cgttcacggt
     1441 ggcgtcggcg tcgccgtgga agacgatggt gggcggcgtg gcgccggcct tgggcgagcg
     1501 gcggggcgcc gtcgggccgc cccccatcgc cgcgaaggcg gtctggacgt cggtggccga
     1561 acccgcgggc aggcccgagt gcacgccgac cgcggcgaac aggtcgggat aggcgtcgcc
     1621 caggatcgcg gccatcgcgc cgccggccga cagtcccgcc acgtacacgc gcgccgggtc
     1681 gatgtcgtgc gagacgacga cctggcgcac catgcccgcg aggatgcccg gctcgccctt
     1741 gccgcgcgcc tggtggttgt gcttgaacca gttccagcag cgctgcgcgt tcgcccgctg
     1801 cgactgcgcc ggatacagca cgaggaagcc ccgggcccgc gcgagcgcgt tcatgcgcgt
     1861 gccgcgcgcg aagtcgtcgg gatcctgcgt gcagccgtgc agcatcacga tgagcggcag
     1921 cggcctcggg tccggccgcg ccggctcgaa cagcttgtag tcgcgcgtgc cgcagtcgtt
     1981 gcggaaatgt ccggcgcaga acgactccgg ccggctcgcc ggcatcgtgc ggggcgggac
     2041 cgccggctcg tcgatgaccc gcgcctgcac gtcgatgacg tcctcgtccg cgaccgccat
     2101 gcccccgcgc ggcgcgggtg gccgggcgcc gagcgcctgc tggatggccg cggtcgcgtc
     2161 catcaggctg ccgccgcgca ccaggcgcgc ggcctcctcc atccgtcgct ggaattcctc
     2221 gttcatgacc tcttccgatc ggccgacgcg gccttcacgg ccacatgggc acgccacgcg
     2281 cccggcgcgg gcagcaggtt cgagccacgc gatcgatcag ggcgcacgcc gcatctcctg
     2341 gtggacgata cccgcttcct cgaagcgctc gccgtgcggc cggaagcccg cgcgacggta
     2401 gaagccgaac gccgtgatct gggcgtgcaa cgccacttcc gggtccccgc gctgcccggc
     2461 ggcggccatc agggcctcca gcatcgcgcg gccgacgttg gcgccgcgca cggcccggtt
     2521 gacggccatg cggccgatgc gcgccgggtg gccggcaggg tcggcggcca gcaggcggcc
     2581 ggtgcccacg gccaggccga gccggttgcg cgccagcgcg tggacggccg acacgtcggc
     2641 ggcgtcgcgc tcgagcgcca gcggcacctt ctgctcctcg acgaacaccg ccgtgcggat
     2701 cgcctgcgcc tcggcgccga gctcgctcca gccgccgacc ctgacgtcga ccatggactc
     2761 gccggattcg aacccgcgga acgcgtcgac cagcgcctgc ggggccggcc tgggcttctt
     2821 cgagac
//
LOCUS       DATCRO010000057         9014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_855765, whole genome shotgun sequence.
ACCESSION   DATCRO010000057 DATCRO010000000
VERSION     DATCRO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855765"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VIN75_01500"
     CDS             <1..403
                     /locus_tag="VIN75_01500"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY8972862.1"
                     /translation="RRSLRADYRYYWDTWNVRGHTLELAYNRNLAPGWLLESFVRYYR
                     QNHALFYSDNAPTQTLYVSRNRELATMHDFGPGVTVSYTLRSIPGKYDVKLSGTYQFI
                     DFHYADYTIPDTGKPYSYHASVFNTVLSATF"
     gene            425..1108
                     /locus_tag="VIN75_01505"
     CDS             425..1108
                     /locus_tag="VIN75_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8972863.1"
                     /translation="MLKLTRTALIAFGLCAAAALSPVLAADAPAAAASAPLPAAVVDA
                     MPAVAPATAASAAAPMAAASAPDAAASAAASEPAPTGSLDDRVQQTKADVIRLNRDLL
                     VLEEELLFPANTQVAVFVSMDVGLLFDLDSVQVKLDDKVVTTYLYTPLEVQALHRGGV
                     QRVYLGDLKAGPHEIVAFFTGKGPHDRDYKRATTIKFDKSTEPKYIELQIKDVQQKLQ
                     PEFDVKVWQ"
     gene            1114..3042
                     /locus_tag="VIN75_01510"
     CDS             1114..3042
                     /locus_tag="VIN75_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972864.1"
                     /translation="MRLRLRHLPSVRLVAALVAGVVATAGVRAADDPNKVPADPPKHP
                     VVDPHYGDVLFEFYQGRYFPAITKLEVSQHFDRMQHHADEAEILRGGLLLSYGLHKQA
                     AEVFEALLARGAPPSVRDRAWYFLAKIRYQRGLMSEASAALGRVEHVLPAPLEDDRKL
                     LQANVAMALGDNDAAGKILTPIAGPRGTDLYARYNLGVAMIRSGDVAHGTNVLDDLGR
                     QTMPDEESRALRDKANVALGFAAMKDGKPMLARDYLQRVRLQGIQANAALLGFGWAAD
                     AVGNPKQALVPWLELAGRDPSDPSVLEAQLAVPYAYAELHADAQALQRYENAIANFEN
                     EDANIDATIAAIREGKLVDGLMARNPGDDMGWFQTFRDLPLMPHASLLTPVIAGNEFQ
                     EAFKNYRDLQFLTHNLTEWHDNLGVFGDMLATRRQAFAERLPRIREQQQARGLGLDQL
                     QKRSDANAADLKSAEDAADGVAFADASERELQQRLARVTDTLAKLPADADPGLADARE
                     RLRRVAGALAWQLAHEYPARAWSAKKGQKQTEDALADARQHDADLARAQQEEPVRFED
                     FARRIAELDARLNVLLPRVAALSREQQGALQEIAVTQLKAQKERLAAYTAQARYAVAQ
                     LYDRATEPKEEGSHADKP"
     gene            3026..5956
                     /locus_tag="VIN75_01515"
     CDS             3026..5956
                     /locus_tag="VIN75_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8972865.1"
                     /translation="MPTSPDRLRPLLRLAPLPRALALVVLTGSLGACGVMGGAKKPGA
                     TPDNAPTIKSLLAKEVVIDQDPGIPADEDKAIAAYRNFLAVTPDAKQRADALRRLGDL
                     SMASADNANAAHPTTTGVPDYSAAIAQYRDYLKNYPNDPNNDRVLYQLARAQEQGGAL
                     EESLKTLDELVARYPKTHYYDEVEFRRGEILFTLRQYAKAEAAYTLVLKGDYDNPYRE
                     RSLYMQGWSKFKQGNLEEALSSFFGVLDAKIVGIRDDDLSQSDKLTRADKELVDDTLR
                     VIGISLANLKGAESIPPYMTDAGRRGYEFRIYQQLGQLYLKQERPKDAADAYAGFARL
                     NPLDAQAPVMQAKVIAIDQDAGFDQLALSAKKDYVAQYGLDGEFRKVNPEGWEKAQPL
                     VKTALAELAQRYHAQAQKTKAKDDYVEAAKWYRAWLAEFGSDPDAAENNFLLAEVLYE
                     SGQYSASAIEYEKTAYEYPAHPHGADAGYSALLAHAAEQKGVTGDALLPLQRAGVDSE
                     LRFAAAFPNDSRTPAVLTNAADTLFKLHDNDRAAGVAEQVLALDPPATPAQRRVAWTL
                     VAHTSFEAGKFDKAEKAYGEVLALVPEKDPARGELTERLAASIYKQGEQARAAGDNKA
                     AAENFARVAVAAPGSAIRVNAEYDAAASLIALKDWPAAAKALEAFRQRYPNHALQGDV
                     TANLALAYSEQKQWAPAAAQYERIAQAPATTPDAQNRARIALWQAATLYERAAGSDPD
                     SVKAGKPKPADAGTNRAAAAKDYERYLKQYPTPLEPAVEAHYRLAQLAHIDGNAAVEN
                     AHMHAIFVADQNGGSARTDRTKTLGAMAALAAAQPSYEAYQKVALVEPLAKNLKLKKA
                     KMEDALKAYAVASDYGVAEVTTAATYHVANVYQDFAKALTSSQRPKKLSKLELEQYNE
                     MLEEQADPYVLKAIELHGINASRTTQGIYDDWVKKSFEALKTLQPARYNKSEHSEATV
                     DAIR"
     gene            5943..6746
                     /locus_tag="VIN75_01520"
     CDS             5943..6746
                     /locus_tag="VIN75_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8972866.1"
                     /translation="MRSAERLRLALTAAAAAALLAGCGSVKFMSRGTEPDASTAASAA
                     AQAASAAASVPKAAASAVAGPAPASTGPIAPATQRAYDDALALMRAGHVADAEKGLRA
                     LTQSNPELAGPHANLGVIARNAGRLPEAVSELEKATTLAPGHAVAWDQLGLTYRQAGE
                     FTKARDAYEHALAINPDYATAVLNLGVLEDLYLAEPAKALELYTRYLALVPGGDPVVT
                     KWQADVRRRVPGAATAAASAASAPAAAPAPAPAPAPAARSANASKEKKS"
     gene            6743..7063
                     /locus_tag="VIN75_01525"
     CDS             6743..7063
                     /locus_tag="VIN75_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972867.1"
                     /translation="MTARRPLACLLTIGCLVAPAFGQDRAEIDPTKIIGNRELPKVLY
                     IVPWKKPLPGDLAGRPPVSVVDEALAPVDRDVFRRQVRYDKLEQEHAAAVAPPAAATP
                     PAAH"
     gene            7120..7770
                     /locus_tag="VIN75_01530"
     CDS             7120..7770
                     /locus_tag="VIN75_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8972868.1"
                     /translation="MDTLNSIVKFFQDCGWFIFPSLAIMATGLAIAIERFVFLQKARV
                     ANRQDWARVLPLLQGGQWKEVLGITAASDSAIGKIVHNGLSRLQSTRRRDDIDAAMEE
                     GMMEIVPRLEKRTHYIAVFANVITLVGLLGTITGLIHAFTAVAQVNPAEKAALLSASI
                     SIAMNNTAFALMVAIPFLLIHSFLQARTADIVDGLEGAKISFLNLVQRLAPEHKAA"
     gene            7813..8307
                     /locus_tag="VIN75_01535"
     CDS             7813..8307
                     /locus_tag="VIN75_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8972869.1"
                     /translation="MPVRRLRKKSEALEVSAFINLIVVLVPFLLSTAVFSRMSVIDLT
                     LPAKSANYEGLKENDLKLEVVIRHDAIEVGDKIGGLIQRIPNTTDNHVDTTTLNGVMF
                     VVKNKFPTVTAASVLPEPNIPYETVVQVMDAMRTGKTANGGTTVVDVDLFPAISIGDA
                     PVRH"
     gene            8321..8809
                     /locus_tag="VIN75_01540"
     CDS             8321..8809
                     /locus_tag="VIN75_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8972870.1"
                     /translation="MKLTPVQKRAARNARHGNSLDMNLVSLIDVFTILIFFLLSNSGV
                     ETLPAASVHLPESTAKSDPKQTLVVAVNANEIVVEGRKVADVAPLMAANATDDVIPGL
                     KAELDAIAARPVAQAENAATAHSVTIMGDKDIPYQLLRKVMYTAARADFTNVSFAVNR
                     KD"
     gene            8814..>9014
                     /locus_tag="VIN75_01545"
     CDS             8814..>9014
                     /locus_tag="VIN75_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8972871.1"
                     /translation="MTAISFRQSTLPWALAHDDEERFRKLFLALMGFVIVFGIALRFI
                     TLPEPERAPVATLPPIAAKLLLD"
BASE COUNT         1559 a         3342 c         2950 g         1163 t
ORIGIN      
        1 ccgccgctcg ctgcgcgccg actaccgcta ctactgggac acgtggaacg tccgcggcca
       61 cacgctggag ctcgcgtaca accgcaacct cgcgcccggc tggctgctcg agagcttcgt
      121 gcgctactac cgccagaacc acgcgctgtt ctacagcgac aacgcgccca cgcagacgct
      181 gtacgtgtcg cgcaaccgcg agctcgcgac gatgcacgac ttcggccccg gcgtgaccgt
      241 gtcgtacacg ctgcgcagca tcccgggcaa gtacgacgtc aagctgagcg gcacgtacca
      301 gttcatcgac ttccactacg ccgactacac gatccccgac accggcaagc cgtactcgta
      361 ccacgcgagc gtgttcaaca cggtgctgtc ggccacgttc taagaaaaga caagagagat
      421 cagcatgctc aaactgactc gcacggcgtt gatcgccttc ggactgtgcg ccgccgcggc
      481 gctgtcgccc gtgctggccg ccgatgctcc ggccgccgcg gcttccgccc cgctgccggc
      541 cgccgtcgtc gacgcgatgc ccgccgtcgc acccgccacc gccgcttcgg ccgccgcgcc
      601 catggccgcc gcgtccgcgc ccgatgccgc ggccagcgcc gccgcgagcg agccggcgcc
      661 caccggcagc ctggacgacc gcgtgcagca gaccaaggcc gacgtgatcc gcctgaaccg
      721 cgacctgctg gtgctcgagg aggaactgct gttcccggcc aacacgcagg tggcggtgtt
      781 cgtgtcgatg gacgtcggcc tcctgttcga cctcgactcg gtgcaggtca agctcgacga
      841 caaggtcgtc accacctacc tgtacacgcc gctggaggtg caggcgctgc atcgcggcgg
      901 cgtccagcgc gtctacctcg gcgacctgaa ggccggcccg cacgagatcg tggcgttctt
      961 caccggcaag ggcccgcacg accgcgacta caagcgcgcc accacgatca agttcgacaa
     1021 gagcaccgag cccaagtaca tcgagctgca gatcaaggac gtgcagcaga agctgcagcc
     1081 ggaattcgac gtcaaagtct ggcagtgacg acgatgcgct tgcgcctgcg ccaccttcct
     1141 tccgttcgcc tggtcgccgc gctggtcgcg ggcgtcgtcg cgacggcggg cgtgcgcgcc
     1201 gccgacgacc cgaacaaggt gccggccgat ccgcccaagc acccggtggt ggatccgcac
     1261 tacggcgacg tgctgttcga gttctaccag ggccgctact tcccggcgat caccaagctc
     1321 gaggtgtcgc aacacttcga ccgcatgcag caccacgccg acgaggccga gatcctgcgc
     1381 ggcggcctgc tgctgtccta cggcctgcac aagcaggcgg ccgaggtgtt cgaggcgctg
     1441 ctggcgcgcg gcgcgccgcc gtcggtgcgc gatcgcgcgt ggtacttcct ggccaagatc
     1501 cgctaccagc gcggcctgat gtccgaggca tccgcggcgc tgggccgcgt cgagcacgtg
     1561 ctgcccgcgc cgctggagga cgaccgcaag ctgctgcagg ccaacgtggc gatggcgctc
     1621 ggcgacaacg acgccgccgg caagatcctg acgccgatcg ccggcccccg cggcaccgac
     1681 ctgtacgcgc gctacaacct gggcgtggcg atgatccgca gcggcgacgt cgcgcacggc
     1741 acgaacgtgc tggacgacct cggccgccag accatgcccg acgaggaaag ccgcgcgctg
     1801 cgcgacaagg ccaacgtcgc cctcggcttc gccgcgatga aggacggcaa gcccatgctc
     1861 gcgcgcgact acctgcagcg cgtgcgcctg cagggcatcc aggccaacgc cgccctgctg
     1921 ggcttcggct gggcggcgga cgccgtcggc aaccccaagc aggcgctggt gccctggctg
     1981 gagctggccg gccgcgaccc gagcgacccg tcggtgctcg aggcgcagct ggccgtgccc
     2041 tacgcgtacg ccgagctgca cgccgacgcg caggcactcc agcgctacga gaacgcgatc
     2101 gcgaatttcg agaacgagga cgccaacatc gacgcgacca tcgccgcgat ccgcgaaggc
     2161 aagctcgtcg acggcctgat ggcccgcaac cccggcgacg acatgggctg gttccagacc
     2221 ttccgcgacc tgccgctgat gccgcacgcg agcctgctca cgccggtgat cgccggcaac
     2281 gagttccagg aagcgttcaa gaactatcgc gacctgcagt tcctcacgca caacctgacc
     2341 gagtggcacg acaacctggg cgtgttcggc gacatgctcg ccacgcgccg ccaggcattc
     2401 gccgagcggc tgcccaggat ccgcgaacag cagcaggcgc gcggcctggg cctcgatcag
     2461 ctgcagaagc gcagcgacgc caacgccgcc gacctcaagt ccgccgagga cgccgccgac
     2521 ggcgtcgcct tcgccgacgc aagcgagcgc gagctgcagc agcggctggc acgcgtcacc
     2581 gacacgctgg ccaagctgcc cgccgatgcc gaccccggcc tcgcggacgc gcgcgagcgc
     2641 ctgcgccgcg tggcgggcgc gctcgcgtgg cagctggcgc acgaataccc ggcccgtgca
     2701 tggagcgcga agaagggcca gaagcagacc gaggacgcgc tcgcggacgc gcgccagcac
     2761 gacgccgacc tcgccagggc gcagcaggag gagcccgtgc gcttcgagga cttcgcgcgc
     2821 cgcatcgccg agctcgacgc ccgcctgaac gtgctgctgc cgcgcgtggc cgcgctgtcc
     2881 agggaacagc agggcgcgtt gcaggagatc gccgtcaccc aactgaaggc gcagaaggag
     2941 cgcctggccg cctacaccgc gcaggcccgc tacgccgtcg cgcagctgta tgaccgcgcc
     3001 accgaaccga aggaggaggg cagccatgcc gacaagccct gaccgccttc gaccgctgct
     3061 gcgcctggcg ccgctgccgc gcgcgctcgc gctcgtcgtg ctcaccggct cgctcggtgc
     3121 gtgcggagtg atgggcggcg ccaagaagcc gggtgccacg ccggacaacg cgccgaccat
     3181 caagtcgctg ctggccaagg aagtggtcat cgaccaggat ccgggcatcc cggccgacga
     3241 ggacaaggcg atcgccgcct accgcaactt cctggccgtc acgcccgacg cgaagcagcg
     3301 cgccgacgcg ctgcgccgcc tgggcgacct gtcgatggcc agcgccgaca acgccaacgc
     3361 cgcgcacccg acgaccaccg gcgtgcccga ctactccgcg gccatcgcgc agtaccgcga
     3421 ctacctgaag aactacccga acgacccgaa caacgaccgc gtgctgtacc agctcgcgcg
     3481 ggcgcaggag cagggcggcg cgctcgagga gtcgctcaag acgctggacg agctcgtcgc
     3541 caggtacccg aagacgcact actacgacga ggtcgagttc cgccgcggcg agatcctgtt
     3601 cacgctgcgc cagtacgcca aggccgaggc cgcctacacg ctggtgctca agggcgacta
     3661 cgacaacccg taccgcgagc gctcgctgta catgcagggc tggtccaagt tcaagcaggg
     3721 caacctcgag gaggcgctca gctcgttctt cggcgtgctg gacgccaaga tcgtgggcat
     3781 ccgcgacgac gacctcagcc agtccgacaa gctcacccgc gccgacaagg aactggtgga
     3841 cgacacgctg cgcgtgatcg gcatctcgct ggccaacctc aagggcgccg agtcgattcc
     3901 gccctacatg accgacgccg gccggcgcgg ctacgagttc cgcatctacc agcagctggg
     3961 ccagctgtac ctgaagcagg agcgcccgaa ggacgcggcc gacgcctatg ccggcttcgc
     4021 gcgcctgaat ccgctcgacg cccaggcgcc ggtcatgcag gccaaggtca tcgccatcga
     4081 ccaggacgcc ggcttcgacc agctggcgct gtcggccaag aaggactacg tcgcgcagta
     4141 cggcctggac ggcgagttcc gcaaggtcaa ccccgagggc tgggagaagg cgcagccgct
     4201 ggtcaagaca gcgctggccg aactggccca gcgctatcac gcgcaggcgc agaagaccaa
     4261 ggccaaggac gactacgtcg aggccgcgaa gtggtaccgc gcgtggctgg ccgagttcgg
     4321 cagcgacccg gacgccgcgg agaacaactt cctgctggcc gaggtgctgt acgagagcgg
     4381 ccagtattcg gcctctgcga tcgagtacga gaagacggcc tacgagtacc cggcgcatcc
     4441 gcacggcgcc gacgcgggct actccgcgct gctcgcgcat gccgccgaac agaagggcgt
     4501 caccggcgac gcgctgttgc cgctgcagcg cgcgggcgtc gacagcgagc tgcgcttcgc
     4561 ggccgcattc cccaacgact cgcgcacgcc ggcggtgctc accaacgccg ccgacacgct
     4621 gttcaagctg cacgacaacg accgcgccgc gggcgtcgcc gagcaggtgc tcgcgctcga
     4681 cccccccgcc acgccggcgc agcgccgcgt ggcctggaca ctggtggcgc acacgtcgtt
     4741 cgaggccggc aagttcgaca aggccgagaa ggcctacggc gaggtgctgg cgctcgtgcc
     4801 cgagaaggat ccggcacggg gcgagctgac cgagcgcctg gcggcgtcga tctacaagca
     4861 gggcgagcag gcccgcgccg cgggcgacaa caaggccgcg gccgagaact tcgcgcgtgt
     4921 cgcggtggcc gcgcccggct cggcgatccg cgtgaacgcc gagtacgacg cggcggcctc
     4981 gctgatcgcg ctgaaggact ggcccgccgc ggccaaggcg ctggaggcgt tccgccagcg
     5041 ctatccgaac cacgcgctgc aaggcgacgt cacggccaac ctggcgctgg cctacagcga
     5101 gcagaagcag tgggcgcccg cggccgcgca gtacgagcgc atcgcgcagg ccccggccac
     5161 gacgccggac gcgcagaacc gcgcccgcat cgcgctgtgg caggcggcga cgctgtacga
     5221 gcgcgccgcc ggcagcgacc cggacagcgt caaggccggc aagcccaagc ccgccgatgc
     5281 cgggaccaac cgcgccgccg ccgccaagga ctacgagcgc tacctcaagc agtaccccac
     5341 gccgctggaa cccgcggtcg aggcgcacta caggctcgcg cagctggcgc acatcgacgg
     5401 caacgccgcg gtggagaacg cgcacatgca cgccatcttc gtggccgacc agaacggcgg
     5461 ctcggcgcgc accgaccgca cgaagacgct cggcgcgatg gccgcgctgg ccgcggcgca
     5521 gccgtcgtac gaggcctacc agaaggtcgc gctcgtggaa ccgctggcca agaacctgaa
     5581 gttgaagaag gcgaagatgg aggacgcgct caaggcgtac gcggtggcca gcgactacgg
     5641 cgtcgccgag gtcaccacgg ccgcgacgta ccacgtggcc aacgtctacc aggacttcgc
     5701 caaggcgctc acgtcgtcgc agcgtcccaa gaagctgtcc aagctggaac tcgagcagta
     5761 caacgagatg ctcgaggagc aggccgatcc gtacgttctc aaggcgatcg agctgcatgg
     5821 catcaacgcg agccgcacca cgcagggcat ctacgacgac tgggtcaaga agagcttcga
     5881 ggcgctcaag acgctgcagc cggcgcgcta caacaagtcc gaacacagcg aggcgaccgt
     5941 cgatgcgatc cgctgaacgc ctacgcctgg cgctgacggc cgccgcggcc gccgcgctgc
     6001 tcgccggctg cggctcggtg aaattcatgt cgcgcggcac ggagccggac gcctccaccg
     6061 cggcgtcggc cgccgcccag gccgccagcg ccgcggcgtc cgtgcccaag gccgccgcca
     6121 gcgcggtcgc cggaccggcg ccggcgtcca ccggtccgat cgcgcccgcc acgcagcgcg
     6181 cgtacgacga cgcgctggcc ctgatgcgcg ccggccacgt cgccgacgcc gagaagggct
     6241 tgcgcgcgct gacgcaatcg aaccccgagc tcgccggccc gcacgccaac ctgggcgtga
     6301 tcgcccgcaa cgccggccgc ctgcccgaag ccgtcagcga actcgagaag gccacgacgc
     6361 tggcccccgg ccatgccgtc gcctgggacc agctggggct gacctatcgc caggccggcg
     6421 agttcacgaa ggcgcgcgac gcctacgagc acgcgctggc catcaacccc gactacgcca
     6481 ccgcggtgct gaacctgggc gtgctggaag acctgtacct ggccgagccg gccaaggcgc
     6541 tggagctgta cacgcgctac ctcgcgctgg tgcccggtgg cgatccggtc gtcaccaagt
     6601 ggcaggccga cgtgcggcgc cgcgtgccgg gcgccgcgac ggcggccgcc tccgccgcat
     6661 ccgcgcccgc cgccgcgccg gcgcccgcac cggccccggc gccggctgca aggtcggcca
     6721 acgcgtccaa ggagaagaag tcatgaccgc acgacgcccg ctcgcctgcc tgcttacgat
     6781 cggttgcctt gtcgcaccgg cgttcggcca ggatcgcgca gaaatcgatc ccacgaagat
     6841 catcggcaac cgagaattgc cgaaggtgct ctacatcgtg ccgtggaaga agccgctgcc
     6901 cggcgacctc gccggccggc cgccggtgag cgtggtcgac gaggcgctgg cgcccgtcga
     6961 tcgcgacgtc ttcaggcgcc aggtgcgcta cgacaagctc gaacaggagc acgccgccgc
     7021 ggtggcgcct ccggccgcgg cgaccccgcc cgcggcgcac tgaatccgca tcgtcccctt
     7081 tcgtttcacg ttcccgactc tgttcccgga gatttccaga tggataccct caactcgatc
     7141 gtcaagttct tccaggactg cggctggttc atcttcccga gcctcgcgat catggccacg
     7201 ggcctcgcga tcgccatcga gcgcttcgtc ttcctgcaga aggcccgcgt ggccaaccgc
     7261 caggactggg cgcgcgtgct gccgctgttg cagggcggcc agtggaagga agtgctgggc
     7321 atcaccgcgg cctccgattc ggccatcggc aagatcgtgc acaacggcct gtcgcgcctg
     7381 cagtcgacgc gccgccgcga cgacatcgac gccgcgatgg aggaaggcat gatggagatc
     7441 gtgccgcgcc tggaaaagcg cacgcactac atcgccgtgt tcgccaacgt catcacgctg
     7501 gtgggcctgc tgggcaccat cacgggcctg atccacgcgt tcaccgccgt ggcccaggtc
     7561 aacccggccg agaaggcggc gctgctgtcg gcgtccatct cgatcgccat gaacaacacg
     7621 gccttcgcgc tgatggtcgc gatcccgttc ctgctgatcc actcgttcct gcaggcccgc
     7681 accgccgaca tcgtcgacgg actggaaggc gccaagatca gcttcctgaa cctcgtccag
     7741 cgcctggccc cggaacacaa ggcggcttga tcatgcgccg ttccacccac ccgcaaaccc
     7801 gggggtgccg ccatgcctgt tagacgcctg cgcaagaagt ccgaggcgct ggaggtcagc
     7861 gcgttcatca acctgatcgt cgtgctggtg ccgttcctgc tgtcgacggc ggtgttctcg
     7921 cgcatgtcgg tgatcgacct gacgttgccc gccaagtcgg ccaactacga aggcctcaag
     7981 gagaacgacc tcaagctcga ggtcgtgatc cgccacgacg ccatcgaggt cggcgacaag
     8041 atcggcggcc tgatccagcg catcccgaac acgaccgaca accacgtcga caccaccacg
     8101 ctcaatggcg tgatgttcgt cgtcaagaac aagttcccga ccgtcaccgc ggcgtccgtg
     8161 ctgcccgagc ccaacatccc gtacgagacc gtcgtccagg tgatggacgc gatgcgcacg
     8221 ggcaagacgg ccaacggcgg caccacggtg gtcgacgtcg acctgttccc ggcgatctcc
     8281 atcggcgacg cgccggtgcg gcattgaaag ggggcccgcg atgaagctca cccccgtcca
     8341 gaagcgcgcc gcgcgcaatg cccgccacgg caactcgctc gacatgaacc tcgtgtcgct
     8401 gatcgacgtc ttcaccatcc tgatcttctt cctgctgtcg aactccggcg tcgagacgct
     8461 gcccgccgcc tccgtgcacc tgcccgagtc caccgccaag tccgatccga agcagacgct
     8521 ggtggtagcg gtcaacgcca acgagatcgt cgtcgagggc cgcaaggtcg ccgacgtcgc
     8581 gccgctgatg gcagccaacg ccaccgacga cgtcatcccg ggcctgaagg ccgagctcga
     8641 cgccatcgcg gcccgcccgg tggcccaggc cgagaacgcc gccacggcgc actcggtgac
     8701 gatcatgggc gacaaggaca tcccgtacca gttgctgcgc aaggtgatgt acaccgccgc
     8761 gcgcgccgac ttcacgaatg tctcgttcgc cgtcaaccgg aaggactgac ggcatgaccg
     8821 caatctcctt ccgccaatcg acgctcccct gggcgctcgc gcacgacgac gaggaacgct
     8881 tccgcaagct gttcctcgcg ctgatgggct tcgtgatcgt gttcggcatc gcgctgcgct
     8941 tcatcacgct gcccgagccc gagcgcgcgc ccgtggccac gctgccaccc atcgctgcca
     9001 agctgctgct ggac
//
LOCUS       DATCRO010000058        10612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_720774, whole genome shotgun sequence.
ACCESSION   DATCRO010000058 DATCRO010000000
VERSION     DATCRO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720774"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VIN75_01550"
     CDS             <1..981
                     /locus_tag="VIN75_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972872.1"
                     /translation="EPAPQEAAHDDLPLVDEGDARGVPIVDHSATRPLDRHALAAAVA
                     PAGTPRELSVEELLDLEQQADFFIALGQEDAAVDLLMSHLRSAGGQSPLPYTKLLEIY
                     RRQGDRAAYERTRARFNRRFNAYAPDWDTGPSAGRSLEDYPETIAQLERAWHSPLDAM
                     AILESLLFKRDDTSELFDLPAYRDVLVLYSLARDLWQHGGGHGGTQIDVLLPLDDGVA
                     HAASVTSYPSAREVNERALPEEDAGLDLESVDVEGDTRHGMLTSFEMTGFDSGPSGSD
                     LDLELPPSGGDVHLTLDELSLSPAAPARGKPDPRNWRDGDGPDSEPPTKR"
     gene            complement(985..2148)
                     /locus_tag="VIN75_01555"
     CDS             complement(985..2148)
                     /locus_tag="VIN75_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8972873.1"
                     /translation="MTSDDLVGASRLPSVGTTIFTVMSALAARTGAINLGQGFPDFDC
                     DPALVDAVAAAMRAGHNQYPPMTGIPALRQAVSDKIALTTGHRYDADAEITITAGATQ
                     GILSTVLALVHPGDDVIVLEPCYDSYGPSITLAGGNVIRVPLVPGTWRPDFDALAKAL
                     TPRTRLVIVNTPHNPSGTVWTSAERARLAALLAPTRALVLSDEVYEHMVFDGVEHASM
                     SGLPELAARSVVVSSFGKTFHVTGWKVGYVAAPAALMREFRKVHQFNVFTVNTPMQHG
                     IAAFLADASHWRTLAPFYQAKRDRFRAGLAATPLKLLPCEGTYFQCVDYSALSDKDDL
                     AFCEWLATDVGVAAIPMSAFHDQAPDLRVIRFCFAKKEATLDEALRRLQALRA"
     gene            complement(2138..3085)
                     /locus_tag="VIN75_01560"
     CDS             complement(2138..3085)
                     /locus_tag="VIN75_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEY8972874.1"
                     /translation="MNLHQFRFVQEAVRRNLNLTETAKALFTSQPGISKAILELEEEL
                     GIDLFVRHGKRLRRVTEPGQEVLKAIDVILREVANMKRIGEEYSKQDAGTLSIAATHT
                     QARYVLPEPVAQLRRRFPKVQVSLHQGSPSEVVQMLLDGTADIGLATESLAEPEDLVS
                     LPAYEWEHVIVMRADDPLAMVDKISLEQLASETLISYHPSFTGRRRIDAAFAQRRLKP
                     NIALEAIDSDVIKTYVKSGLGVGIVAEMAVRDDPAGGELVSRPAGHLFGQNVTRVAFR
                     RGAYLRGYVLTFAELLSDRLTRPLLLRALGGDGEETYDL"
     gene            complement(3082..3495)
                     /locus_tag="VIN75_01565"
     CDS             complement(3082..3495)
                     /locus_tag="VIN75_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY8972875.1"
                     /translation="MNQAVILLAHGARDPAWATPFEAVVARVRERAPETLVTLAFLEL
                     MAPSLEEAAADLLARGCTQLAVVPAFLGMGGHVRRDVPAQLERLRRAHPGVSFTLHPA
                     LGETPHVIAALADATIDLAGLGFASAHSPLDGIGT"
     gene            complement(3572..4693)
                     /gene="lptG"
                     /locus_tag="VIN75_01570"
     CDS             complement(3572..4693)
                     /gene="lptG"
                     /locus_tag="VIN75_01570"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY8972876.1"
                     /translation="MKTVRQLLYRDVLWSVFFVTIFFIALFFFIDFVDEMSRVGKNGY
                     TMGAAALAVLLEQPGHVYELFPIAVLIGTIYSMARLASSSEFTILRTAGLGPGRALSL
                     LGVIGLLFAVLTFSLGEYVTPHSEQAAGFLKARFGLGLAVGRTGAWLKEKRAEPDGER
                     SITVNVRGVGKDGELTGVRIFEMDATGREVRSLQAEHAVVPRGRTIKEWQLQDVTETR
                     WIPRVDAAEPRVETRRLAQLAWPTTLTSAVATASILPVQSMGALDLWRYSAHLAEQEQ
                     STQTYRIQFWKRVLYPFACLVMVALALPFAYLHARAGGVSVKVFGGIMLGISFVLLNN
                     VAGHLGMLHEWTPWIAASMPSVIYLLMSMSAFAWLVRYR"
     gene            complement(4690..5793)
                     /gene="lptF"
                     /locus_tag="VIN75_01575"
     CDS             complement(4690..5793)
                     /gene="lptF"
                     /locus_tag="VIN75_01575"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY8972877.1"
                     /translation="MLFDSTVRKELARSFAATLMVILTIFMTNLVIRTLGLAANGAVA
                     PQDVVFIFTLLGLQTLPTILSLSLFIGVILTLGRMYRESEMAIWFSSGIGLARFIRPV
                     VSTSWPIIVLIGILMLFVWPWSYSQYNELRQRFEQRADVLRASPGQFQSSGDGHRVFF
                     VEKAASNAAGGGRNVFIMNDAGTRESVAVSQSGKIENIGDERFLVLDSGHMEQNDKAS
                     GEHTHVAFRNYRVHAGTSSTPSNNAPSPRAVDTVDLMLTGGPAYMGELTWRLGLILGA
                     TNLLFMGISLSAANPRRASNWGLLFAALAFVIYYNLLNLTQAWVGSGRLNIVVALVGL
                     HGAAFLLGLGLIWWREHATVIHPAKLLLRRSAA"
     gene            5830..7281
                     /locus_tag="VIN75_01580"
     CDS             5830..7281
                     /locus_tag="VIN75_01580"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8972878.1"
                     /translation="MDFQTLALPEAGVAGVNADALIVVLPATGDLPKFGAAIDAALAD
                     AVKGGDLERKAGKSVYLPRVAGVKAARVVLAVARDTSPKAFKAAVASALGAVKAGPAR
                     HVAVVGGESTAAHAEALAIATGDATYHYTKTKPSAAPAPALAKVTYVVPKSAVATAKA
                     GLAVGGAIVSGMNLARECANLPANFATPTFLGEQAQAMVKTHGLEVEVLDRKACEKLG
                     MGSFLSVARGSDEPPKFIVMQYKGGAKKDAPVVLVGKGITFDTGGISLKPGPEMDEMK
                     FDMCGAASVLGTVRAVAELKLKVNLVGLIPTCDNMPSGKATKPGDVVTSMSGQTIEIL
                     NTDAEGRLILCDALTYAERFKPQAVVDIATLTGAIVIALGNHNSGLFSPDDALAADLL
                     AASAAGLDPAWRMPLDDEYDELLKSNFADMANIGGRAGGSITAAKFLQRYTSKFPWAH
                     LDIAGTAHRSGAAKGATGRPVPLLTHFVMSRAK"
     gene            7373..7819
                     /locus_tag="VIN75_01585"
     CDS             7373..7819
                     /locus_tag="VIN75_01585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY8972879.1"
                     /translation="MTEIAFHTGIANPLDYACRLLRKAYRSGARVAVHGEPALLDRLD
                     QALWTFEQLEFVPHIVLPRDAGDARRVAMTPIVLVKGAATAPAGCRIGVSLAGHPIGD
                     VAAYDRLIAIVGLDPGHREAARLRWREYERAGHAVSHVAKPGGDPA"
     gene            complement(7953..8117)
                     /locus_tag="VIN75_01590"
     CDS             complement(7953..8117)
                     /locus_tag="VIN75_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972880.1"
                     /translation="MTKLLAALIVSTFAVGAFAADAASAPASAPAKKAAKAEHKAAKK
                     AEAASAAASK"
     gene            8285..9538
                     /gene="icd"
                     /locus_tag="VIN75_01595"
     CDS             8285..9538
                     /gene="icd"
                     /locus_tag="VIN75_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY8972881.1"
                     /translation="MSEHVKVPAGGQKIKVNPDFSLDVPDQPIIPYIEGDGTGLDITP
                     VMLKVVDAAVEKSYGGKRRIHWMEIYAGEKATQVYGPDVWLPDETLEIVRDYIVSIKG
                     PLTTPVGGGIRSLNVALRQQLDLYVCLRPVQYFKGVPSPVKHPEKVDMVIFRENSEDI
                     YAGIEYEAESDKARKLIKFLQDEMGVKKIRFPETSGIGIKPVSREGTERLVRKALQYA
                     IDNDKPSVTLVHKGNIMKFTEGGFRDWGYALAQKEFGAQLIDGGPWVKFKNPKTGRDI
                     TVKDSIADAFLQQIILRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANMSDSVA
                     MFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIIASMERAILSKKVTY
                     DFARLMDGATQVSCSGFGQVMIDHM"
     gene            9679..10107
                     /locus_tag="VIN75_01600"
     CDS             9679..10107
                     /locus_tag="VIN75_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8972882.1"
                     /translation="MTPSSPDFQQFMRRRADIALAYVNGDAAPLGEVVTRREPAHFFG
                     PNGGHADGVDAVDRDYRAGAAHFEAGGESRVEVLQAQDSGDLAYWVGLQHARVRVKGK
                     PDPVPMDLRVTEIYRREEGGWKLVHRHADTLAEPQHQLAD"
     gene            10433..>10612
                     /locus_tag="VIN75_01605"
     CDS             10433..>10612
                     /locus_tag="VIN75_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication/partition related protein"
                     /protein_id="HEY8972883.1"
                     /translation="MNIVVREDLKSYIEPLTPDEHDALERSLLAEGCRDALVLWGDVL
                     VDGHNRYQICQQHGIP"
BASE COUNT         1712 a         3643 c         3711 g         1546 t
ORIGIN      
        1 gagcccgcgc cgcaggaagc cgcccacgac gacctgccgc tcgtcgacga gggcgacgcc
       61 cgtggcgtgc cgatcgtcga ccactcggcc acccgtccgc tcgatcgcca tgcgctcgcg
      121 gccgcggtgg ccccggcggg cacgccgcgc gagctgtcgg tcgaggagct gttggacctc
      181 gagcagcagg ccgacttctt catcgcgctc ggccaggagg acgcggccgt cgacctgttg
      241 atgtcgcacc tgcgcagcgc cggcggccag agcccgctgc cctacaccaa gctgctggag
      301 atctaccgtc gccagggcga ccgcgctgcc tacgagcgca cgcgggcccg cttcaaccgc
      361 cgcttcaacg cctacgcccc ggactgggac accggcccga gcgccggccg ttcgctcgag
      421 gactaccccg agaccatcgc gcagctcgag cgcgcatggc actcgccgct ggacgcgatg
      481 gcgatcctgg aatcgctgct gttcaagcgt gatgacacca gcgagctgtt cgacctgccg
      541 gcctaccgcg acgtgctcgt gctgtactcg ctggcgcgcg acctgtggca gcacggcggc
      601 ggccacggcg gcacgcagat cgacgtgctg ctgcccctgg acgacggcgt cgcccacgcc
      661 gccagcgtga cctcctaccc gagcgcccgc gaggtcaacg agcgcgcgct gcccgaggaa
      721 gatgccggcc tcgacctgga gtcggtcgac gtcgagggcg atacccgcca cggcatgctc
      781 accagcttcg agatgaccgg cttcgactcc ggtccgtcgg gcagcgacct cgacctcgag
      841 ctgccgccgt cgggcggcga cgtgcacctg acgctcgacg agctgagcct gtcgccggcc
      901 gcgcccgcac gcggcaagcc cgatccgcgc aactggcgcg acggcgacgg ccccgattcc
      961 gagccgccga ccaagcgctg agcgtcaggc ccggagcgcc tgcaggcggc gcagcgcctc
     1021 gtccagcgtc gcttccttct tggcaaagca gaagcggatg acgcgcaggt cgggcgcctg
     1081 gtcgtggaag gccgacatcg ggatcgcggc cacgcccacg tcggtggcca gccattcgca
     1141 gaacgcgaga tcgtccttgt ccgacagcgc cgaatagtcg acgcactgga agtacgtgcc
     1201 ctcgcagggc agcagcttca ggggcgtggc ggccagcccg gcgcggaaac ggtcgcgctt
     1261 ggcctggtag aacggcgcga gcgtgcgcca gtgcgacgca tcggccagga aggcggcgat
     1321 cccgtgctgc atcggcgtgt tgacggtgaa cacgttgaac tggtgcacct tgcggaattc
     1381 gcgcatcagc gccgcgggcg ccgccacgta gccgaccttc cagccggtga cgtggaaggt
     1441 cttgccgaag ctcgacacca cgacgctgcg cgcggccagt tcgggcaggc ccgacatgct
     1501 cgcgtgctcg acgccgtcga acaccatgtg ctcgtagacc tcgtcggaca gcaccagcgc
     1561 gcgggtcggg gcgagcaggg cggccaggcg cgcgcgctcg gcggacgtcc ataccgtgcc
     1621 gctcgggttg tgcggcgtgt tgacgatgac cagccgcgtg cgcggcgtca gcgccttggc
     1681 gagcgcgtcg aagtcgggcc gccacgtgcc cggcaccagc ggcacgcgga tcacgttgcc
     1741 gccggccagc gtgatcgacg ggccgtagct gtcgtagcac ggctcgagca cgatcacgtc
     1801 gtcgcccgga tgcaccagcg cgagcaccgt cgacaggatg ccctgcgtgg cgccagcggt
     1861 gatggtgatc tcggcgtcgg cgtcgtagcg atggccggtg gtcagcgcga tcttgtccga
     1921 cacggcctgg cgcagcgcgg ggatgcccgt catcggcggg tactggttgt ggccggcgcg
     1981 catggccgcg gcgaccgcgt cgacgagcgc gggatcgcag tcgaagtcgg ggaagccctg
     2041 gccgaggttg atcgcgcccg tcctggcggc cagcgccgac atcacggtga agatggtggt
     2101 gcccacgctg ggcagccggc tcgcgccgac gaggtcgtca gaggtcatag gtctcttctc
     2161 cgtcgccgcc gagggcgcgc agcagcaagg ggcgcgtgag gcggtcggac agcagctcgg
     2221 cgaacgtgag gacgtagccg cgcaggtacg cgccgcggcg gaacgccacg cgcgtgacgt
     2281 tctggccgaa caggtgcccg gcaggacggc tgacgagctc gccgccggcc gggtcgtcgc
     2341 gcactgccat ctcggcgacg atgccgacgc ccagccccga cttcacgtag gtcttgatga
     2401 cgtcggagtc gatggcctcg agcgcgatgt tgggcttgag gcggcgctgc gcgaaggcgg
     2461 cgtcgatgcg gcggcggccg gtgaacgacg ggtggtagga gatcagcgtc tcgctggcga
     2521 gctgttccag cgagatcttg tcgaccatgg ccagcgggtc gtcggcgcgc atgacgatca
     2581 cgtgctccca ctcgtaggcg ggcagcgaga cgagatcctc gggctcggcc agcgactcgg
     2641 tggccaggcc gatgtcggcc gtgccatcga gcagcatctg cacgacctcg ctgggcgagc
     2701 cctggtgcag gctgacctgc accttgggaa agcgccggcg cagctgcgcc accggctcgg
     2761 gcaggacgta gcgcgcctgc gtgtgggtgg cggcgatcga cagcgtgccg gcgtcctgct
     2821 tcgaatactc ctcgccgatg cgcttcatgt tggcgacctc gcgcaggatc acgtcgatcg
     2881 ccttgagcac ctcctggccc ggctccgtga cgcggcgcag acgcttgccg tggcgcacga
     2941 acaggtcgat gcccagctcc tcctcgagct cgaggatggc cttggagatg ccgggctgcg
     3001 aggtgaacag ggccttggcc gtctcggtca ggttcaggtt gcggcgcacg gcctcctgca
     3061 cgaatcggaa ctggtgcagg ttcatgtgcc gatcccgtcg agcgggctgt gggcggacgc
     3121 gaagccgagt ccggccaggt cgatggtggc gtcggcgagc gcggcgatca cgtgcggcgt
     3181 ctcgccgaga gccggatgca gcgtgaacga cacgcccgga tgcgcccggc gcaggcgctc
     3241 cagctgcgcc ggcacgtcgc gccgcacgtg gccgcccatg ccgaggaagg ccggcacgac
     3301 ggccagctgc gtgcagccgc gcgcgagcag gtcggcggcg gcctcttcca gcgagggcgc
     3361 catcagctcg aggaacgcga gcgtcaccag ggtctcgggc gcgcgctcgc gcacgcgggc
     3421 gacgacggcc tcgaacggcg tggcccaggc cgggtcgcgc gcgccgtggg ccaggaggat
     3481 gacggcctga ttcatgtgtg cacccctggc gctgcgcgcc ttcccgccag gtgcgggtca
     3541 tcgcccttcg gacagccgtg cgggcgcggg ctcatcggta cctcaccagc cacgcgaacg
     3601 ccgacatcga catcagcagg tagatcacgc tgggcatcga cgcggcgatc cacggtgtcc
     3661 attcgtgcag catgcccagg tggcccgcca cgttgttcag cagcacgaag ctgatgccca
     3721 gcatgatgcc gccgaacacc ttcacgctga cgccgccggc gcgcgcgtgc aggtaggcga
     3781 acggcagcgc gagcgccacc atcaccaggc acgcgaacgg gtagagcacg cgcttccaga
     3841 actggatgcg gtaggtctgc gtcgattgct cctgttcggc caggtgcgcg ctgtagcgcc
     3901 acaggtccag cgcgcccatg ctctgcaccg gcaggatgga ggcggtggcg acggccgagg
     3961 tgagggtggt gggccacgcc agctgcgcga ggcgcctggt ctcgacgcgc ggctcggcgg
     4021 cgtccacgcg cgggatccag cgcgtctcgg tcacgtcctg cagctgccat tccttgatcg
     4081 tgcggccgcg cggcaccacg gcgtgctcgg cctgcaggct gcgcacctcg cggccggtgg
     4141 cgtccatctc gaagatgcgc acgccggtga gctcgccgtc cttgccgacg ccgcgcacgt
     4201 tgacggtgat ggagcgttcg ccgtcgggct cggcgcgctt ttccttcagc catgcgccgg
     4261 tgcgtcccac cgccaggccc aggccgaacc gcgccttcag gaagcccgcg gcctgctcgc
     4321 tgtgcggcgt gacgtactcg cccaggctga acgtgaggac cgcgaacagc aggccgatca
     4381 cgcccagcag gctcagcgcg cgcccgggcc cgaggccggc ggtgcgcagg atggtgaact
     4441 cggacgacga cgccagccgc gccatcgagt agatcgtgcc gatcagcacc gcgatgggga
     4501 acagctcgta gacgtggccc ggctgctcca gcagcaccgc gagcgcggcg gcccccatgg
     4561 tgtagccgtt cttgccgacg cggctcatct cgtcgacgaa gtcgatgaag aagaacagcg
     4621 cgatgaagaa gatggtcacg aagaacacgg accacaggac gtcgcggtac agcagctggc
     4681 ggacggtctt catgcggcgc tccgtcgcag cagcagcttg gccgggtgga tgacggtcgc
     4741 gtgctcgcgc caccagatca gtcccagccc gagcaggaac gcggcgccgt gcaggcccac
     4801 cagcgcgacc acgatgttga gcctgcccga gccgacccag gcctgggtca ggttgagcag
     4861 gttgtagtag atgacgaagg ccagcgctgc gaacagcagg ccccagttgc tggcgcggcg
     4921 cgggttggcg gccgacaggc tgatgcccat gaacagcagg ttggtcgcgc ccaggatcag
     4981 gccgagccgc caggtgagct cgcccatgta ggccgggccg ccggtcagca tcaggtcgac
     5041 ggtgtcgacg gcgcgcgggg agggcgcgtt gttggacggc gtgctgctgg tgccggcgtg
     5101 cacgcggtag ttgcggaacg cgacgtgcgt gtgctcgccc gaggccttgt cgttctgctc
     5161 catgtggccc gagtcgagca cgaggaagcg ttcgtcgccg atgttctcga tcttgcccga
     5221 ctgcgacacc gccaccgatt cgcgcgtgcc cgcgtcgttc atgatgaaga cgttgcgccc
     5281 gccgccggcc gcgttgctgg ccgccttctc gacgaagaac acgcggtggc cgtcgcccga
     5341 cgactggaac tggccgggcg acgcgcgcag cacgtcggcg cgctgctcga agcgctggcg
     5401 cagctcgttg tactggctgt agctccacgg ccagacgaac agcatcagga tgccgatgag
     5461 cacgatgatg ggccagctgg tggacaccac cggccggatg aagcgcgcca ggccgatgcc
     5521 gctggagaac cagatggcca tctcgctctc gcggtacatg cgtccgagcg tgaggatcac
     5581 gccgatgaac agcgacagcg acaggatggt gggcagcgtc tgcaggccca gcagcgtgaa
     5641 gatgaagacg acgtcctgcg gcgccaccgc gccgttggcc gccaggccca gcgtgcggat
     5701 gaccaggttg gtcatgaaga tggtcaggat caccatcagc gtcgcggcaa agctgcgcgc
     5761 caactccttg cggaccgtcg aatcgaataa catcgcgcga ttatctccaa tcgactgcga
     5821 atccttacca tggacttcca aaccctggcc ctgcccgaag cgggcgtcgc cggcgtcaat
     5881 gccgacgcac tcatcgtcgt cctgcctgcc accggcgacc tgcccaagtt cggggccgcc
     5941 atcgacgcgg cgctggccga cgccgtcaag ggcggcgacc tcgagcgcaa ggccggcaag
     6001 tcggtgtact tgccgcgcgt ggccggcgtg aaggcggccc gcgtggtgct ggccgtcgcg
     6061 cgcgacacct cgcccaaggc cttcaaggcg gccgtcgcgt cggcgctggg cgccgtcaag
     6121 gcgggcccgg ccaggcacgt ggccgtcgtc ggcggcgaat ccaccgcggc ccacgccgag
     6181 gcgctggcca tcgccacggg cgacgccacg taccactaca cgaagaccaa gcccagcgcc
     6241 gcgccggcgc cggcgctggc caaggtgacc tacgtcgtgc ccaagtcggc cgtcgccacc
     6301 gccaaggcgg gcctggccgt gggcggcgcg atcgtctcgg gcatgaacct cgcgcgcgag
     6361 tgcgcgaacc tgccggccaa cttcgccacg cccaccttcc tgggcgagca ggcgcaggcc
     6421 atggtcaaga cgcacggcct ggaggtcgag gtgctcgatc gcaaggcgtg cgagaagctg
     6481 ggcatgggct cgttcctgtc cgtcgcacgc ggttccgacg aaccgccgaa gttcatcgtc
     6541 atgcagtaca agggcggcgc caagaaggac gcgccggtcg tgctggtggg caagggcatc
     6601 acgttcgaca ccggcggcat ctcgctcaag ccgggccccg agatggacga gatgaagttc
     6661 gacatgtgcg gcgccgccag cgtgctgggc accgtgcgcg cggtggccga gctcaagctc
     6721 aaggtgaacc tggtcggcct gatcccgacc tgcgacaaca tgccctcggg caaggccacc
     6781 aagccgggcg acgtcgtcac cagcatgtcg ggccagacca tcgagatcct gaacaccgac
     6841 gccgaagggc gcctcatcct gtgcgacgcg ctgacctacg ccgagcgctt caagccgcag
     6901 gccgtcgtcg acatcgccac gctcaccggc gccatcgtca tcgcgctggg caaccacaac
     6961 agcggcctgt tctcgccgga cgacgcgctc gccgccgacc tgctggccgc ctcggccgcc
     7021 ggcctcgacc cggcctggcg catgccgctg gacgacgagt acgacgagct cctcaagagc
     7081 aacttcgccg acatggccaa catcggcggc cgcgccggcg gctcgatcac cgcggccaag
     7141 ttcctgcagc gctatacgtc caagttcccg tgggcccacc tggacatcgc cggcacggcc
     7201 catcgttcgg gcgcggccaa gggggcgacc ggccgtccgg tgccgctgct gacccacttc
     7261 gtcatgagcc gcgcgaagtg attgtcggcc gcctggccgt tcccgtcgcc gtccccgagg
     7321 aggcgcgcgg atcggtccgg gtggtgacgc tcgcttcccg cgaccgatcc ccttgacgga
     7381 aattgctttc cacaccggca tcgcgaatcc actggattac gcgtgccggc tgctgcgcaa
     7441 ggcctatcgg tcgggcgcgc gcgtggccgt ccacggcgag ccggcgctgc tcgatcgcct
     7501 ggaccaggcc ctgtggacgt tcgagcagct ggagttcgtg ccgcacatcg tgctcccgcg
     7561 cgacgccggc gacgcgcgcc gggtggcgat gacgcccatc gtgctggtca agggcgcggc
     7621 cacggcgccg gcgggatgcc gcatcggcgt gtcgctggcg ggacacccca tcggcgacgt
     7681 cgcggcctac gaccgcctga tcgcgatcgt gggactcgat cccgggcatc gggaagcggc
     7741 gcgcctgcgc tggcgcgaat acgagcgcgc aggccatgcc gtgagccatg tcgccaagcc
     7801 gggcggcgac ccggcttgat ccgcctcgac gggtggttcg gagcgcggat cgggaatcgg
     7861 ccccgccaat gaaaacgccc ggcgcgaggc cgggcgttcc ctcttttcga agccgctccg
     7921 gcaagccggc gcgacccaga aagattcgaa gattacttcg aagcagcggc cgaagcggct
     7981 tccgccttct tcgcagcctt gtgctcggcc ttggcggcct tcttggcggg ggccgaagcc
     8041 ggagccgaag cggcgtcagc ggcgaaggcg ccaacggcga aggtggaaac gatcagggcg
     8101 gccaggagct tggtcatttt aaggtacctt aatgaagttg aaattgcttt cccgtcaggg
     8161 cgtagcagcc cttgaacggt tccccctgtc gagcggttga cagttttttt cgttctagaa
     8221 tttatttttt tccgagggtc ttcacggagg ccccgtcacc cagaacaacg cggagcaaga
     8281 gtcgatgagc gaacacgtca aggtcccggc gggcgggcag aagatcaagg tcaacccgga
     8341 cttctcgctg gacgtgcccg accagcccat catcccgtac atcgagggtg acggcacggg
     8401 cctggacatc acgccggtga tgctcaaggt ggtggacgcg gcggtcgaga agtcctatgg
     8461 cggcaagcgc aggatccact ggatggagat ctacgccggc gagaaggcca cgcaggtcta
     8521 cggccccgac gtctggctgc cggacgagac tctcgagatc gtccgcgact acatcgtctc
     8581 catcaagggt ccgctgacga cgccggtggg cggcggcatc cgctcgctga acgtggcgct
     8641 gcgccagcag ctcgacctgt acgtctgcct gcgcccggtg cagtacttca agggcgtgcc
     8701 ctcgccggtc aagcatccgg agaaggtgga catggtgatc ttccgcgaga actcggagga
     8761 catctacgcc ggcatcgagt acgaggccga aagcgacaag gcgaggaagc tgatcaagtt
     8821 cctgcaggac gagatgggcg tcaagaagat ccgcttcccc gagacctcgg gcatcggcat
     8881 caagccggtc tcgcgcgagg gcaccgagcg cctggtgcgc aaggcgctgc agtacgcgat
     8941 cgacaacgac aagccctcgg tcacgctggt gcacaagggc aacatcatga agttcacgga
     9001 aggcggcttc cgcgactggg gctacgcgct ggcgcagaag gaattcggcg cccagctgat
     9061 cgacggcggc ccgtgggtga agttcaagaa cccgaagacg ggccgcgaca tcaccgtcaa
     9121 ggactcgatc gccgacgcct tcctgcagca gatcatcctt cgcccggccg aatactcggt
     9181 catcgcgacg ctcaacctca acggcgacta catctccgac gcgctggccg cccaggtcgg
     9241 cggcatcggc atcgccccgg gtgccaacat gtccgactcg gtcgcgatgt tcgaggccac
     9301 gcacggcacc gcgcccaagt acgccggcaa ggactatgtc aatccgggct ccgagatcct
     9361 gtcggccgag atgatgctgc gccacatggg ctggaccgag gcggccgacc tgatcatcgc
     9421 ctcgatggag cgcgcgatcc tcagcaagaa ggtcacctac gacttcgccc gcctgatgga
     9481 tggcgccacg caggtctcgt gctccggctt cggccaggtg atgatcgacc acatgtgacc
     9541 cgtggcggtc gcccgaccgc cggcgatggc ttccaggacc ccgggccgac ctgcgtcggc
     9601 cggggtcttt ttcatgggcc gcccgcgtgc cgccggggcg cggggattgc tgccatggca
     9661 ctttcaacgg agcaagccat gacgccatca tctcccgatt tccagcagtt catgaggcgg
     9721 cgcgcggaca tcgcgctggc ctatgtcaac ggcgatgccg caccgctggg cgaggtcgtc
     9781 acccggcgcg agccggcgca cttcttcggc ccgaacggcg gccatgccga cggcgtcgac
     9841 gcggtggatc gcgactaccg cgccggcgcc gcgcacttcg aggccggtgg ggagagccgg
     9901 gtcgaggtgt tgcaggccca ggacagcggc gacctggcct actgggtcgg actgcagcat
     9961 gcgcgcgtcc gcgtgaaggg caagcccgat ccggtgccga tggacctgcg cgtcaccgag
    10021 atctaccggc gcgaggaggg cggctggaaa ctggtgcacc ggcacgcgga cacgttggcc
    10081 gagccgcagc accagctcgc ggactgagcc gttccgggcg ccccggagac ttatttcgga
    10141 gagaagcgac tcgaagaggc gcgttcaagc cattgatcgg ctgccgccgc ggcgccgggc
    10201 cgggttgctt cctgtgcgcc tcggaaaatg aaagtgtcac gcgaaaaatc gagcgcaagc
    10261 cattgttaaa caacaaaaat ctctgatcac ttcgcctggg ccgacagcgc cggctggccg
    10321 gcgcggtgcc ggtggagcgc tgacgcccgt agaatggcac ccattccctg acgaaggccc
    10381 gcctgcgcgc gggccttcgc gtctctgcag cccctgaacc ccgcaccgcc ccgtgaacat
    10441 cgtcgtccga gaagacctca agagctacat cgaaccgctg acccccgacg agcacgacgc
    10501 gctcgagcgc agtctgctgg ccgaaggctg ccgcgacgcg ctggtgctgt ggggcgacgt
    10561 gctggtcgat ggccataacc gttaccagat ctgccagcag cacggcatcc cg
//
LOCUS       DATCRO010000059         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_563272, whole genome shotgun sequence.
ACCESSION   DATCRO010000059 DATCRO010000000
VERSION     DATCRO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563272"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VIN75_01610"
     CDS             complement(<1..1988)
                     /locus_tag="VIN75_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY8972884.1"
                     /translation="MKQRNPSSRAPARRLQLRPIALSLICAGAAPAMAQVLPTGFTPL
                     AGNVTMSQAANVMTINQGLARGIVNWTSFSIGVGGVVNIVQPGASSVLLNRVTGNELS
                     TIAGQLNANGRIVLVNPAGVLFSQGSTVNVGGIIASTLAMTTSEADFLNPATTKFSFE
                     RADASTASVINNGRITATGGGPVVLMGATVANARIGSDDDSGRITADGGTVALASGRK
                     ITLDLAGDGLTNIVIATDAMATHASVSNSGTLQADGGRVALVGRSATVGELVVNQRGT
                     VRARSVGTRNGEIFLGGGEDNEVQLTGGTLDATGAAAGERGGDIRVLGGKVTVQGLAD
                     GTKGVADASGQAGGGSVTVQGYDVALSPNAVLRADGTGAGAGGTIGVGTATGAAFAGD
                     AANPLPERESHVQVFGELTARGASSAAGGSITTSGDFVRIDPARVDAAGGAQGGANGR
                     WTVSTGNNLTVDADADMPAYDGAYPSLSFSDVSARAVGATLGRGTDVTLSSTRTGDSA
                     GVTFGNDAQVVKNEGGSATLRVDSAGGIAMNSGSAIDARVGALNVDFNADSTGLVAKN
                     AAQVNVGGGNPSFELDYGASIIMSGATIDANGGNIRFFGQGDANNGRAVGGSLSHSEL
                     TWSDGIFLSNSTLATSGAGQVSLRGQGRTRVDSQDSDT"
     gene            complement(2244..>3851)
                     /locus_tag="VIN75_01615"
     CDS             complement(2244..>3851)
                     /locus_tag="VIN75_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY8972885.1"
                     /translation="LPTKDPCGSCDRPIAQAPTGAVAPQSLPAPPRNTGGAAGATFKL
                     NDLRLNGVKALSNEELQAITGPYIGRDVTLSDLEELAKAITARYKERGYFLAQAVVPV
                     QTVRDGIVEISVIEGRLGKVDVTVAPDAPIAESRVRGFLAPLQPGAAVSAPDYERAML
                     LLSDQPGIKVSSGLQEGTQPGTTDLSVEVAAAPRWAFTAEGDNHGTKESGRYRVGGTA
                     RWLSPFGIGDNLDMRLMVSNSNALQFGRIAYEAPIGTSGLRAGVGLSRVSYELGGQFA
                     DLDARGRANVLDFSLSYPLIRQRQQNLFLRLGVDVKDLTDELRAADFNSKKRVNGLSL
                     GWTWERRDELLGGGYWASSGTLYHGNLSIRDPESRDFDRSTTGHNTAGGFTKLSFQFS
                     RLQAIVPRHSLYLSVGGQWASKNLDASEKLSLGGARAVRAYPSGELLVDQGVIGTVEW
                     RWSFNEELTPFLFYDAAHGKIVRNPTPYDGVNSHSLRGYGVGLSWSRPGNFSINATLA
                     WRAGTPPAQTDGGGRNPRLFVQLIKAF"
BASE COUNT          517 a         1338 c         1380 g          616 t
ORIGIN      
        1 ttggtgtcgc tgtcctgcga gtcgacccgc gtgcgtccct ggccgcgcag cgagacctgc
       61 ccggcgccgc tggtcgcgag cgtactgttg ctcaggaaga tgccgtcgct ccacgtcagt
      121 tcgctgtgcg acagagaacc gccgaccgcg cgcccgttgt tcgcgtcgcc ctgaccgaag
      181 aaacggatgt tgccgccgtt ggcgtcgatg gtcgcgccgc tcatgatgat cgatgcaccg
      241 tagtcgagtt cgaagctcgg gttgccgccg ccgacgttca cctgcgcggc attcttcgcc
      301 accagcccgg tcgagtcggc gttgaagtcg acgttgagtg cacccacgcg cgcgtcgatg
      361 gccgaaccgc tgttcatcgc gatgccgccg gccgagtcga cgcgcagcgt ggcgctgccg
      421 ccctcgttct tgaccacctg cgcatcattg ccgaaggtca cgcccgcgct gtcgccggta
      481 cgggtgctcg acagcgtcac gtcggtgccg cgcccgagcg tggcgcccac ggcgcgcgcg
      541 ctcacgtcgc tgaaagacag ggagggatag gcgccgtcgt aggccggcat gtcggcatcc
      601 gcgtccacgg tgaggttgtt gccagtcgac accgtccatc gcccgttggc cccgccctgc
      661 gcgccgcctg cggcgtcgac acgcgccggg tcgatgcgca cgaagtcgcc gctcgtggtg
      721 atgctgcccc cggcagcgct gctggcgccc cgcgcggtga gttcgccgaa gacctgcaca
      781 tgcgactcgc gctccggcag cgggttggcg gcatcgcctg cgaaggcagc gccggtggcg
      841 gtgcccacgc cgatcgtgcc gccggcgcct gcgcccgtgc cgtcggcgcg cagcaccgcg
      901 ttcggcgaaa gtgccacgtc gtagccctgc accgtgaccg agccgccacc ggcctggccg
      961 ctggcatcgg ccacgccctt ggtgccgtcg gccagcccct gcacggtcac cttgccgccc
     1021 agcacgcgga tgtcgccgcc gcgttcgcct gcggcggcgc cggtggcgtc gagcgtgccg
     1081 ccggtgagct gcacttcgtt gtcctcgcca ccgcccagga agatctcgcc gttgcgcgta
     1141 cccacgctgc gcgcgcgcac cgtgcctcgc tggttcacca ccagctcgcc gaccgtggcc
     1201 gagcggccga cgagcgccac gcgcccgcca tcggcctgga gggtgccgct gttgctcacg
     1261 ctggcgtgcg tggccatcgc atcggtcgcg atgacgatgt tggtcaggcc gtctccggcc
     1321 aggtcgagcg tgatcttgcg gcccgaggcc agcgcgacgg tgccgccgtc ggccgtgatg
     1381 cggccgctgt cgtcatcgct gccgatgcgg gcattggcca ccgtggcgcc catcaacacc
     1441 accgggccgc cgcccgtcgc agtgatgcgg ccgttgttga tgaccgatgc cgtgctggca
     1501 tccgcacgct cgaaactgaa cttcgtcgtc gccgggttga ggaaatcggc ttcgctggtc
     1561 gtcatggcca gcgtggacgc gatgatgcct cccacgttga ccgtggagcc ctggctgaac
     1621 agcaccccgg cggggttgac cagcacgatg cgcccgttgg cgttgagctg gcccgcgatg
     1681 gtggaaagct cgttgcccgt cacacggttg agcagcacgc tggaagcgcc cggctgcacg
     1741 atgttcacca caccgcccac gccgatggag aagctcgtcc agttgacgat gccgcgcgcc
     1801 aggccctggt tgatggtcat cacgttcgcc gcctgcgaca tggtgacgtt gccggccagc
     1861 ggcgtgaaac ccgtgggcag cacctgcgcc atggcgggcg ccgcacccgc gcagatcagc
     1921 gacagcgcga tgggccgcag ctgcaggcgc cgcgcgggag ctcgcgaaga tggattgcgt
     1981 tgtttcattt cagtgttcca cgacgttctc gcttgagcgc aatgcatgaa accggcgtgc
     2041 gcaattcgcg ggacaccgcg gaaccggctc tgccgggccg ctggtgtcgc cccggcgagg
     2101 gggttggcga agcgacacga agtgcgcgaa gcctggggga gaaccgggtc tccgcggaac
     2161 cggctctgcc ggccgctggt gtcgccccct ggaggggagt ggcgaagcga cacgaagtgc
     2221 gcgaagcctg ggggagtgcc atgtcagaac gccttgatga gctgcacgaa cagccgcggg
     2281 ttgcggcccc cgccatcggt ctgcgcgggc ggcgtgccgg cgcgccaggc cagcgtcgcg
     2341 ttgatcgaga aattgcccgg gcgcgaccag ctcaggccca cgccgtagcc gcgcaggctg
     2401 tggctgttga cgccgtcgta gggcgtgggg ttgcgcacga tctttccgtg cgcggcgtcg
     2461 tagaacagga agggcgtgag ctcctcgttg aacgaccagc gccattcgac ggtgccgatc
     2521 acgccctggt ccaccagcag ttcgcccgag gggtaggcgc gcaccgcgcg cgcgccgccc
     2581 agcgagagct tctccgacgc gtcgaggttc ttgctggccc actgcccgcc caccgacagg
     2641 tacagcgaat ggcgtggcac gatggcctgc aggcgcgaga actggaagct cagcttggtg
     2701 aagccgcccg cggtgttgtg gcccgtggtg ctgcggtcga agtcgcggct ctcggggtcg
     2761 cggatggaga ggttgccgtg gtacagcgtg ccggagctgg cccagtagcc gccgccgagc
     2821 agttcgtcgc gccgctccca cgtccagccc aggctcaggc cgttgacgcg cttcttcgaa
     2881 ttgaagtcgg ccgcgcgcag ctcgtcggtg aggtccttca cgtccacgcc caggcgcagg
     2941 aagaggttct gctggcgctg gcggatcagc ggatagctca gcgagaagtc gagcacgttg
     3001 gcgcgcccgc gcgcgtcgag gtcggcgaac tgcccgccca gctcgtagct cacgcgcgag
     3061 aggccgacgc ccgcgcgcag cccgctggtg ccgatgggcg cctcgtaggc gatgcggccg
     3121 aactgcagcg cgttgctgtt ggacaccatc agccgcatgt cgaggttgtc gccgatgccg
     3181 aagggactga gccagcgcgc ggtgccgccc acgcggtagc ggcccgattc cttggtgccg
     3241 tggttgtcgc cctcggcggt gaaggcccag cgcggcgcgg cggccacctc gaccgagaga
     3301 tcggtggtgc cgggctgcgt gccttcctgc aggcccgacg acaccttgat gcccggctgg
     3361 tccgacagca gcagcatcgc gcgttcatag tccggcgcgc tcaccgccgc gccgggctgc
     3421 agcggcgcga ggaagccgcg cacgcgcgac tcggcaatgg gcgcatcggg agcgacggtc
     3481 acgtcgacct tgcccaggcg gccctcgatc acgctgatct cgacgatccc gtcgcgcacg
     3541 gtctgcaccg gcaccacggc ctgcgcgagg aagtagccgc gctccttgta gcgcgcggtg
     3601 atcgccttgg cgagctcctc caggtcggag agcgtgacgt cgcggccgat gtaggggccg
     3661 gtgatggcct gcagttcttc gttgctcagc gccttcacgc cgttgaggcg caggtcgttg
     3721 agcttgaagg tcgcgccggc ggcgccgccc gtgttgcgcg gcggcgcggg caggctctgc
     3781 ggggcgacgg cccccgtggg tgcctgcgcg atggggcggt cacagcttcc gcaagggtcc
     3841 ttggtcggca g
//
LOCUS       DATCRO010000060         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_675792, whole genome shotgun sequence.
ACCESSION   DATCRO010000060 DATCRO010000000
VERSION     DATCRO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675792"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VIN75_01620"
     CDS             <1..215
                     /locus_tag="VIN75_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972886.1"
                     /translation="DEWGPAYAYGVAHYNRNVATNPGATFEELEPELERGWEEARGKS
                     SMEWTRARLAAYDAWQNARAADDRFN"
     gene            complement(243..428)
                     /locus_tag="VIN75_01625"
     CDS             complement(243..428)
                     /locus_tag="VIN75_01625"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEY8972887.1"
                     /translation="MPILRLEMHPGRTPEQKRDFVREATRVAVEILKCPPESVDILID
                     EIPRDQWAKGGKLVSEL"
     gene            546..1433
                     /locus_tag="VIN75_01630"
     CDS             546..1433
                     /locus_tag="VIN75_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972888.1"
                     /translation="MDKLAGMEAFVRVVEGGSFAAAAAQVGVSPTMVAKQVQAIENRL
                     GARLLHRTTRRHQLTEIGQLYLERCRAALAGVALAEASALELQRTPRGRLRMVAPVGF
                     GSRTLAPALAAWLAEHPDVSVDLTLDDRPEALMRAGHELGVVIGEVGDPSLVARPLRL
                     YRRLLAAAPSYLAEHGKPRHPRELAQHACLGNTYWKHSDRWHLQGPGGQTCVVPVSAR
                     FTANEGRALRRAAAAGAGIVLQPEDALAGDIAAGRLAPVLPAWSFQPTPMHLVYPQDR
                     KPTAKLRSAIDFLVARLGA"
     gene            complement(1438..>2986)
                     /locus_tag="VIN75_01635"
     CDS             complement(1438..>2986)
                     /locus_tag="VIN75_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEY8972889.1"
                     /translation="YGMGLITSRITGVEFEIGVFLGLGGILVCSFLGGMRAVTWTQVA
                     QYIVLLVAYLVPVAWLSIKQHASPVPQATYGYQLQKVIASEKRLQADPAEQRVIAIFQ
                     ARADADQARLSDIPAALAAERSVATRRIAELKAANAPQGDIQAAERVLARVPRDADAA
                     REQWTRERDADRERAQPLAGMPPHAQQFAGDPHGDAAARRAFDDSRRNFIALVFCLMV
                     GTASLPHVLTRFYTTPSVGEARASVGWSLLFIVLLYLTAPALAVLVKVEVFDALVGTR
                     FDRLPAWINNWAHVDGSLLQVADVNGDGILQLGEIKIGGDIVVLATPEIAGLPYVISG
                     LVAAGGLAAALSTADGLLLTIANALSHDLWFRIVDPKAPATRRVTVSKVLLLMVALGA
                     AYVTAQKPGDILLLVSAAFSIAASAFFPALVMGVFWKRANRWGAAAAMVGGLGLTLYY
                     ALVNWPGVREVFGITGPIRLWGGIQPIAAGLFGVPVGFALLVTVSLLTPPPSRAAREM
                     VDEVRLP"
BASE COUNT          448 a         1083 c         1074 g          381 t
ORIGIN      
        1 acgacgaatg gggcccggcc tacgcgtacg gcgtcgccca ctacaaccgc aacgtcgcga
       61 ccaacccggg cgcaaccttc gaggagctcg agcccgagct cgagcgcggc tgggaagaag
      121 cgcgcggcaa gtccagcatg gaatggacac gcgcccgcct ggccgcctac gacgcgtggc
      181 agaacgcgcg ggcggcggac gatcggttca actgagcccg ccggcgcggg ccggcaccgg
      241 tcctacagct ccgagaccag cttgccgccc ttggcccact ggtcgcgcgg gatctcgtcg
      301 atcaggatgt ccacgctctc gggcgggcac ttgaggatct ccaccgccac gcgcgtggcc
      361 tcgcgcacga aatcgcgctt ctgctccggc gtgcggccgg ggtgcatctc gagtcgaaga
      421 atgggcatgt tcagctccgt gtccgatccg gcgggttgcc ggggttcgac gccagtctcg
      481 gccaacgcgc cgccgcgaaa aacatgcgag gatccaggac attcccaacc tggaggttct
      541 gaatcgtgga caagctcgcg ggcatggagg cgttcgtgcg ggtcgtcgaa ggcggcagct
      601 tcgcggcggc cgcggcgcag gtgggcgtgt ccccgacgat ggtggccaag caggtgcagg
      661 cgatcgagaa tcgactgggc gcgcggctgc tccatcgcac cacccggcgc caccagctca
      721 ccgagatcgg gcagctgtac ctcgagcgtt gccgcgccgc gctggcgggc gtcgcgctcg
      781 cggaggccag cgcgctcgag ctgcagcgaa cgccgcgcgg acgattgcgc atggtcgctc
      841 ccgtgggttt cggcagccgg acgctcgcgc ccgcgctcgc cgcctggctc gcggagcatc
      901 cggacgtgtc ggtcgacctg acgctggacg accgccccga ggccctcatg cgcgccggcc
      961 acgagctggg cgtggtgatc ggcgaggtcg gcgacccctc gctggtggca cgcccgctgc
     1021 gcctgtatcg gcgcctgctc gcggcggcgc cgtcgtacct ggccgagcat ggcaagcccc
     1081 gtcacccgcg cgagctcgcg cagcatgcct gcctgggcaa cacgtactgg aagcactcgg
     1141 accgctggca cctgcagggg cccggcggcc agacctgcgt cgtccccgtg tccgcccgct
     1201 tcaccgcgaa cgaaggccgc gcgctgcgac gcgccgccgc ggcgggggcg ggcatcgtat
     1261 tgcagcccga ggatgcgctg gccggcgaca tcgcggccgg ccggctcgcg cccgtccttc
     1321 ccgcctggtc gttccagccg acgccgatgc acctggtcta tccgcaggac cgcaagccca
     1381 cggccaagct gcgcagcgcg atcgacttcc tcgtcgcgcg cctgggcgcc taggagccta
     1441 gggcagccgc acttcgtcca ccatctcgcg cgccgcgcgg ctggggggtg gcgtcagcag
     1501 gctcaccgtc accagcagcg cgaagccgac cggcacgccg aacaggccgg cggcgatggg
     1561 ctggatgccg ccccagagcc ggatggggcc ggtgatgccg aagacctcgc gcacgcccgg
     1621 ccagttcacg agcgcgtagt acagcgtcag gcccaggccg cccaccatgg cggctgcggc
     1681 gccccaacgg ttggcgcgct tccagaacac gcccatcacg agcgccggga agaaggccga
     1741 cgcggcgatc gagaacgccg ccgacaccag cagcaggatg tcgcccggct tctgcgcggt
     1801 gacgtaagcg gcgcccaggg ccaccatcag caggagcacc ttggacaccg tcacgcgccg
     1861 cgtggccggg gccttcgggt cgacgatgcg gaaccacagg tcgtgcgaca gcgcgttggc
     1921 gatcgtcagc agcaggccgt cggccgtgga cagcgccgcc gccaggccgc ccgcggcgac
     1981 caggcccgag atgacatagg gcaggcccgc gatctcgggc gtggcgagca ccacgatgtc
     2041 gccgccgatc ttgatctcgc ccagctgcag gatgccgtcg ccgttgacgt cggccacctg
     2101 cagcagcgag ccgtcgacgt gcgcccagtt gttgatccag gcgggcagcc ggtcgaaccg
     2161 ggtgcccacc agcgcatcga acacctcgac cttgaccagc accgccagcg ccggcgccgt
     2221 caggtacagc agcacgatga acagcagcga ccagccgacc gacgcgcgcg cctcccccac
     2281 ggaaggcgtc gtgtagaagc gcgtgagcac gtggggcagc gacgccgtgc ccaccatcag
     2341 gcagaacacg agcgcgatga agttgcggcg cgagtcgtcg aacgcgcggc gcgcggcggc
     2401 gtcgccatgc gggtcgcccg cgaactgctg ggcgtgcggc ggcatgccag cgagcggctg
     2461 cgcgcgctcg cggtcggcat cgcgctcgcg cgtccactgc tcgcgcgcgg cgtccgcgtc
     2521 gcgcgggacg cgcgcgagca cgcgctcggc ggcctggatg tcgccttgcg gcgcattggc
     2581 ggccttgagc tcggcgatgc gtcgcgtggc cacgctgcgc tcggcggcga gcgcggcggg
     2641 gatgtcggac aggcgcgcct ggtcggcgtc ggcgcgcgcc tggaagatgg cgatcacgcg
     2701 ctgctcggca gggtcggcct gcaggcgctt ctcggacgcg atgaccttct gcaactggta
     2761 gccgtaggtc gcctggggca ccgggctggc gtgctgcttg atcgacagcc acgccaccgg
     2821 caccaggtag gccaccagca gcacgatgta ctgcgccacc tgcgtccagg tgacggcgcg
     2881 catgccgcca aggaaggagc acaccaggat gccgcccagg ccgaggaaca ccccgatctc
     2941 gaactcgacg ccggtgatgc ggctggtgat cagccccatg ccgtag
//
LOCUS       DATCRO010000061        11150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_372052, whole genome shotgun sequence.
ACCESSION   DATCRO010000061 DATCRO010000000
VERSION     DATCRO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372052"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VIN75_01640"
     CDS             complement(<1..161)
                     /locus_tag="VIN75_01640"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HEY8972890.1"
                     /translation="MLKKIRVDQVRLGMHLHALEGAWMDHPFWKTRFVIRDEEQLKKL
                     QDSVILEVW"
     gene            complement(330..968)
                     /locus_tag="VIN75_01645"
     CDS             complement(330..968)
                     /locus_tag="VIN75_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8972891.1"
                     /translation="MTRTLTLLLALAAFFPTAHAQDVQRGSTKVQMCIGCHGIVGYQA
                     DFPQVYKVPKIAGQDAKYIASALTAYRGGDRKFPTMRSIAASLSDQDIADIAAYYAQL
                     GKPEETVPATLATPVPDKLRTALATCVSCHGPNFSTPTDGTIPRLAGQYSDYLEVALK
                     SYKVEGNPHFGRANAVMGAMAKPLKDDTIAQIADYLASLPGEVRTVPQAKFK"
     gene            1117..1959
                     /locus_tag="VIN75_01650"
     CDS             1117..1959
                     /locus_tag="VIN75_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8972892.1"
                     /translation="MKFEGSSNYVATPDLMLAVNAAMTLRRPLLVKGEPGTGKTMLAE
                     EAAAALGLPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVKDIHNYIVKGVLWQ
                     AFTSATPCALLIDEIDKADIEFPNDLLRELDRMEFHVYETKETIRAVQRPIVFITSNN
                     EKELPDAFLRRCFFHYIKFPEAATMQQIVDVHFPGIKQELVAAALKSFYDVRNLPGLK
                     KKPSTSELLDWLKLLLAEDIPVTALQSKDEKVSIPPLIGALLKNEQDVTLFEKLIFMQ
                     RNNR"
     gene            2090..2677
                     /locus_tag="VIN75_01655"
     CDS             2090..2677
                     /locus_tag="VIN75_01655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8972893.1"
                     /translation="MLNVQPVTLTDGRIRLQPMTLEHVDALKAAAADGELWNLRVTSV
                     PAPDDTRAYVERALSMTDRLPFVVVDLENQNRIVGSTSFHDIVPAIDRVEIGWTWYAR
                     STQRSHVNTCAKLLLLVHAFETLGAKVVGWRTDNYNFASQQAIERLGARKDGVIRHHA
                     LRRDGTVRDTVMYSVTAGEWPEIKAHLRYQQNKPR"
     gene            2743..3549
                     /locus_tag="VIN75_01660"
     CDS             2743..3549
                     /locus_tag="VIN75_01660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8972894.1"
                     /translation="MDEDKNPFPSVLQAALLMLALFLLRAVVGAALYDARDTLGLTEP
                     QCWALETILSNGLMFATVMHALKLTYHELFHPARTSIRGTALALVPRVLLLVPAMVLA
                     VAVVEVVVTRAFPLSEGERALFERMAGNDLATLIVTCLVAPAVEEMFFRGIVLRGFLR
                     RYTRAQAIWCSAALFGLAHLNLYQFVAATLIGALSGWLYTRSRSLVPCIALHASYNTA
                     LAVLATLQPADGAAATLPGILWLASVPLAAVGVWLLRRQFDRQDDSARQA"
     gene            3842..4540
                     /locus_tag="VIN75_01665"
     CDS             3842..4540
                     /locus_tag="VIN75_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972895.1"
                     /translation="MIRSRRPALSALALVTSLLLGGLATNASAVALNNGFSGQSFLDT
                     PLAGTTVAARPELAGTVLADTDTPFTLDGVTGYVQNRVVRETGTGTLDFYWRVVVDPG
                     TNGGTGIDALRLGNFGYGYMTDADWRIDGLGTVGATTGRLFNPAIDPQGDINFLFASD
                     VDAGESSSFFFLHTSATNFALTGMYDVLDADGRFTGIANTYAPAVPEPTPAMLLALGV
                     LTLGVLRSRRARQD"
     gene            4736..5500
                     /locus_tag="VIN75_01670"
     CDS             4736..5500
                     /locus_tag="VIN75_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8972896.1"
                     /translation="MRRLHPCPAERPASWPARPAPLHARIREELREHILSGAWRPDDA
                     LPSERELMAQYGVSRITVRQALGDLQKESLIFKLAGKGAYVSHPRPFQELGRLQGFAE
                     AMEARGHETWNRVLAVEHEPASDLVASRLGLPPRAPVTQVRRVRHLDRQPVSLDITWV
                     PRELGQRLAREDLARRDIFLILENDCATPLGHADLVIDAGLADAALADALEISPGAPM
                     LRIERLTHDTAGRPVDYEHLYCRADNFQYRLRIERR"
     gene            5518..7269
                     /locus_tag="VIN75_01675"
     CDS             5518..7269
                     /locus_tag="VIN75_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY8972897.1"
                     /translation="MDTFTHEVDVLVIGGGTAGPMAAVKARQADPSLRVLLLEKANVK
                     RSGAISMGMDGLNNAVVPGFATREQYVREITAANDGIVNQKTLMAYAQGSFDMIEELD
                     RWGVKFEKDETGDYAMRKVHHLGTYVLPMPEGHDIKKVLYRQLKRARVEITNRLVATR
                     LLTDDQGRIAGAMAFDCRTADFHVIRAKSVVLCTGAAGRLGLPASGYLFGTYENPTNA
                     GDGYSMAYHAGAELSGIECFQINPLIKDYNGPACAYVTGPFGGYTTNARGDRFIECDY
                     WSGQMMWEFQQELQGGRGPVFLKLDHLAEETIAQIETILHTNERPSRGRFHAGRGTDY
                     RERMVEMHISEIGLCSGHSASGVWVDEHARTTVPGLHAAGDLACVPHNYMLGAFVYGR
                     LAGESSAAFCAAHDFAPVDAGQVERERARVWAPLRRERGLPPNQVEYKLRRMVNDYLQ
                     PPKVTRKMEIGLERFEQIREDLGHLHARDPHQLMRAMEVHFIRDCAEMAARASLFRTE
                     SRWGLYHARVDFPARDDAQWFCHAQIRKGDDGAMTSFKRPLEPYIVPMAETERASYQR
                     LRIPAARADSDAAPALA"
     gene            7292..7537
                     /locus_tag="VIN75_01680"
     CDS             7292..7537
                     /locus_tag="VIN75_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8972898.1"
                     /translation="MHSFAPIALHPASVPVSVDPDKCIAHKGCTVCVDVCPLDVLAID
                     LVKGSAYMKYDECWYCMPCEKDCPTGAVHVDIPYLVR"
     gene            7575..8987
                     /locus_tag="VIN75_01685"
     CDS             7575..8987
                     /locus_tag="VIN75_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8972899.1"
                     /translation="MAGALLATALAFAPAAFAGETIRVAIGTQDTTINCATGGLLVRE
                     LHLLEKYLPHDGRYKDVQYDIEWHNFTSGAPLTNEMVADKLDFGAMADFPGSLNGAAF
                     LKAGRKSLFLSVLSGSTIGSGNGIVVPVKSPVQSLADLKGKQISVPFASTSHGMLLRA
                     IKAQGWDPERDVTIVTQAPEVAGSALQAGKIDAHADFVPFADLFPYKGFARKIYDGAQ
                     ADAPTFHGTLVSADYAKKYPEIVVAYLRAVIEANRLFDADPLKYSELVAKVTGIDAEV
                     AYLFGGPLGLQTRDLSWKPEYRQALATAIDTLRLLKRHDSALTADTFIDDSYLRAAFK
                     AEGLDYDAQLKNYARLPLKSSDALTGKPIADDTRAAQVWVAGEPRVRSYASPENAFAD
                     ARKVEAAGGKVRVVYAQDRRLGIKLLADQAWFAADDKGRISAFLLKDDATRWARDNHA
                     RVLDFAAARGFAATHVASAR"
     gene            9001..9846
                     /locus_tag="VIN75_01690"
     CDS             9001..9846
                     /locus_tag="VIN75_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8972900.1"
                     /translation="MSAVLSPDVDAPPARPPAAAALRRRGAGWLAQGAAIVAFVALWH
                     ALAATHAHLGFVDFRNVPTPVAALDAGRDLAHSGKLARHLGYSLWRVGAGFASAAVLG
                     IALGLAIGRFAVVRRALLPPLELLRPIPAVAWIPLAILMFPSAELSMVFITFIGALFP
                     VLLNTLHGVESVDRRLVASARSLGTGRWSLFAQVILPAAAPSIATGLAIGMGTCWFCL
                     VSAEMIAGQFGIGYYTWESYTLQNYGGIIVGMLVIGLLGMGCSALIRWTARLAMPWRR
                     PEDAR"
     gene            9843..10676
                     /locus_tag="VIN75_01695"
     CDS             9843..10676
                     /locus_tag="VIN75_01695"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8972901.1"
                     /translation="MNATVGRIDIEHVHMEVGDGPARFEALHDVSLAIEPGEFVCLLG
                     PSGCGKSTLLGALAGHLAPARGTLRVDGARVDGPHPDRGLVFQQHTLFPWLRVIDNVA
                     YGLKMKGVARAARHARAHALLASVGLAGFEQRWPSQLSGGMQQRVEIARVLINEPRVL
                     LMDEPFGALDALTRSRMQQLLLDVWTRFRTTVVFVTHDIDEALFLADRLLVMSARPGR
                     IVDDLRLQFPRPREPAILTSPDFAGLKRHCLELLHRGESDAPLPRITPLGPPLAASTN
                     P"
     gene            10685..>11150
                     /locus_tag="VIN75_01700"
     CDS             10685..>11150
                     /locus_tag="VIN75_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY8972902.1"
                     /translation="MSFEPVNDPRLASDDPGVRRIAVLDIADAEDDADLPALITALLA
                     DQAAQVRAEAARVLGGWGHPLAVDALAQALLDADAGVRANAATALAQLKDAAAGECLL
                     PWALHDDPAVRAAALRGLKELRIPAAAAPARAALGHRDAGVRREAVGVLGWLK"
BASE COUNT         1695 a         4072 c         3744 g         1639 t
ORIGIN      
        1 atccagactt ccaggatgac gctgtcctgc aacttcttca gctgctcctc gtcgcggatc
       61 acgaagcggg tcttccagaa cggatgatcc atccatgcac cttccagtgc gtggaggtgc
      121 atgcccaggc ggacttggtc gacgcggatc ttcttgagca tggagcgttc gggcaggcga
      181 gacgggagcc ggcccggggc cggcactgcc tggcttttcg gccggatgcg gcgcgactgt
      241 aggggcttct cgacgaatcg caaaaacaaa gccgcccgaa ggcggcttgg cgtgaaggat
      301 tcctgcgcgg cgcgcgggcc gccggcgcct tacttgaact tcgcctgcgg cacggtgcgc
      361 acctcgcccg gcagcgaggc gaggtagtcg gcgatctggg cgatggtgtc gtccttcagc
      421 ggcttggcca tcgcgcccat cacggcattg gcacggccga agtgcggatt gccctcgacc
      481 ttgtaggact tcagcgccac ttcgaggtag tccgaatact ggcccgccag gcgcgggatg
      541 gtgccgtcgg tgggcgtcga gaagttgggg ccgtggcacg acacgcaggt ggcgagcgcg
      601 gtgcgcagct tgtcgggcac cggggtcgcg agcgtggcgg gcaccgtctc ctcgggcttg
      661 ccgagctgcg cgtaataggc ggcgatgtcg gcgatgtcct ggtcggacag cgatgccgcg
      721 atgctgcgca tggtggggaa cttgcggtcg ccgccgcgat aggcggtcag cgccgacgcg
      781 atgtacttcg cgtcctggcc cgcgatcttg ggcaccttgt agacctgcgg gaagtcggcc
      841 tggtagccca cgatgccgtg gcagccgatg cacatctgaa ccttggtcga accgcgctgg
      901 acgtcttgcg cgtgggcggt ggggaagaaa gccgcgaggg ccaggagcaa ggtcagggtt
      961 cttgtcatcg tctcgtcttg gttgatccga acagaggcag tacttccagg caacgggcgc
     1021 atggcccggc tatccaatcg attataggaa cgtcccttta tactggaacg acccactccg
     1081 tcaggcaaaa cccgcccctg ggcgcgcgat cccaccatga agttcgaagg ttccagcaac
     1141 tacgtcgcca cgcccgacct gatgctcgcg gtcaacgccg cgatgacgct gcgccggccg
     1201 ctgctggtca agggcgagcc gggcaccggc aagacgatgc tcgccgagga agccgccgcg
     1261 gcgctgggcc tgccgctgct cgaatggcac atcaagagca cgaccaaggc gcagcagggc
     1321 ctgtacgagt acgacgcggt gagccgcctg cgcgacagcc agctgggcga cccgcgcgtc
     1381 aaggacatcc acaactacat cgtcaagggc gtgctgtggc aggccttcac cagcgcgacg
     1441 ccgtgcgcgc tgctgatcga cgagatcgac aaggccgaca tcgagttccc gaacgacctg
     1501 ctgcgcgagc tcgatcgcat ggagttccat gtgtacgaga ccaaggagac gatccgcgcc
     1561 gtccagcgcc ccatcgtctt catcaccagc aacaacgaga aggagctgcc cgacgcgttc
     1621 ctgcgccgct gcttcttcca ctacatcaag ttccccgagg ccgcgacgat gcagcagatc
     1681 gtcgacgtgc actttcccgg catcaagcag gaactcgtgg ccgcggcgct caagagcttc
     1741 tacgacgtgc gcaacctgcc aggcctgaag aagaagccgt cgacgtccga gctgctggac
     1801 tggctcaagc tgctgctggc cgaggacatc cccgtcacgg cgctgcaatc gaaggacgag
     1861 aaggtctcga tcccgccgct gatcggcgcg ctgctgaaga acgagcagga cgtgacgctg
     1921 ttcgagaagc tgatcttcat gcagcggaac aatcgttgac ccccatgctc ccgctggtcg
     1981 ctgccccccg agggggccgg ccgcgacggg cccggcagag ccggttcccg cgctccctcg
     2041 gtcgggcctg ccgccggcca acgcccttcg aaccgaactg caccctccca tgttgaacgt
     2101 ccaacccgtc accctcaccg acggccgcat ccgcctgcag ccgatgaccc tcgaacacgt
     2161 cgacgcgctc aaggccgcgg cggccgacgg ggagctgtgg aacctgcgcg tcacctcggt
     2221 gcccgcgccc gacgacacgc gggcctacgt cgagcgcgcg ctgtcgatga cggaccgcct
     2281 gcccttcgtg gtggtcgacc tcgagaacca gaaccgcatc gtcggctcga cgagcttcca
     2341 cgacatcgtg cccgcgatcg accgcgtcga gatcggctgg acgtggtacg ccaggagcac
     2401 gcagcgcagc cacgtcaaca cctgcgccaa gctgctgctg ctggtgcatg cgttcgagac
     2461 gctgggcgcc aaggtcgtgg gctggcgcac cgacaactac aacttcgcga gccagcaggc
     2521 catcgagcgc ctgggtgccc ggaaggacgg cgtgatccgc caccacgcgc tgcgccgcga
     2581 cggcacggtg cgcgacacgg tgatgtacag cgtcacggcg ggcgagtggc cggagatcaa
     2641 ggcgcacctg cgctaccagc agaacaagcc gcgctgaggc ggcgcggcaa gcggggcggg
     2701 gcggccgcgc gccaaggcgc gggcgcggcg gcgacatcga gcatggacga ggacaagaac
     2761 ccattcccat cggtcttgca ggcggcgctg ctgatgctgg cgctgttcct gctgcgcgcg
     2821 gtcgtcgggg ccgcgctgta cgacgcgcgc gacacgctgg gcctgaccga gccgcaatgc
     2881 tgggcactcg agaccatcct cagcaacggc ctcatgttcg ccaccgtgat gcacgcgctc
     2941 aagctgacgt accacgagct gttccatccg gcgcgcacgt cgatccgcgg caccgcgctc
     3001 gcgctcgtgc ctcgcgtgct gctgctggtg ccggccatgg tgctggcggt ggcggtcgtc
     3061 gaggtcgtcg tgacgcgcgc gttcccgttg tcagagggcg aacgcgcgtt gttcgagcgc
     3121 atggccggca acgacttggc caccctgatc gtcacgtgcc tggtcgcgcc ggcggtcgag
     3181 gagatgttct tccgcggcat cgtcctgcgc ggcttcctgc gccgctacac ccgggcgcag
     3241 gcgatctggt gctccgcggc cctgttcggc ctggcgcacc tgaacctgta ccagttcgtc
     3301 gcggccaccc tgatcggcgc gctgagcggg tggctctaca cgcgctcgcg ctccctggtg
     3361 ccctgcatcg cgctgcatgc gagctacaac accgcgctgg ccgtgctggc cacgctgcag
     3421 ccggccgacg gcgccgcggc cacgttgcca ggcatcctgt ggctcgccag cgtcccgctc
     3481 gcggccgtcg gcgtgtggct gctgcgacga cagttcgacc ggcaggacga ctcggcacgt
     3541 caagcgtgac ccctgcgcac gcggcgcagg atcgccggca acctggtgtc gtgattggga
     3601 agttcgttga acgggtcccg agcgcagagg agacgttgcc gaatcctctc gcaaacgtcc
     3661 gtgggccagg cggcgcgcgg agcgaggctc gatgcctcgc gacggccacg ggcgttttcg
     3721 aggggattct tcaacgaact tctcaatcac gacactaggg ggctcccctc ccaagcggga
     3781 tggacgcgtg ttcaggcccc gccttaccgt gcaggctccg acacaccacc acggagttgc
     3841 catgatccga tcgcgccgcc cggccctgtc cgccctcgcc ctcgtgacgt ccctgttgct
     3901 tggcggcctc gccacgaacg ccagtgccgt cgccctgaac aacggcttct cgggccagag
     3961 cttcctcgac acgccgctcg ccggcaccac cgtcgccgca cgccccgaac tggcgggcac
     4021 cgtgctcgcc gacaccgaca cgccgttcac gctcgatggc gtcaccggct acgtgcagaa
     4081 ccgcgtcgtg cgcgagaccg gcaccggcac gctggacttc tactggcgcg tcgtggtcga
     4141 cccgggcacc aacggcggca ccggcatcga cgccctgcgc ctgggcaact tcggctacgg
     4201 ctacatgacc gacgccgact ggcgcatcga cggcctgggc acggtgggcg ccaccaccgg
     4261 ccgcctgttc aacccggcca tcgatccgca gggcgacatc aacttcctgt tcgcctcgga
     4321 cgtcgacgcc ggcgagagct cgtcgttctt cttcctgcac accagcgcga ccaacttcgc
     4381 gctgacgggc atgtacgacg tcctcgacgc cgacggcagg ttcaccggca tcgcgaacac
     4441 ctacgcgccg gccgtgccgg agccgacgcc cgcgatgctg ctggcgctcg gcgtgctgac
     4501 gctgggcgtg ctgcgcagcc gccgcgccag gcaggactga agcggccgga gccctctcca
     4561 atgggaccgc ttcgacggcg cggcgctgcc tggaaccgtg cttcgtcgcg ccacgcctga
     4621 actcctgcgg tttccgactt aggcggatcg cgtacgtcca cgcgcgattc gcttcgttcg
     4681 caggcgcccc cgcgcctcct atcgtctcac ctgtcatgac cagctatgac catccgtgag
     4741 acgactgcac ccttgcccgg ctgaacggcc ggcgtcctgg cccgcgcggc ccgcgccgct
     4801 gcacgcccgc atccgcgagg aactgcgcga gcacatcctc agcggcgcct ggcggcccga
     4861 cgatgcgctg ccctccgaac gcgaactgat ggcgcagtac ggcgtcagcc gcatcaccgt
     4921 gcgccaggcg ctcggcgacc tgcagaagga gagcctgatc ttcaagctcg ccggcaaggg
     4981 cgcctatgtg tcgcacccgc gcccgttcca ggagctcggc cggctgcagg gcttcgccga
     5041 ggccatggag gcgcgcggcc acgagacctg gaaccgcgtg ctcgcggtgg aacacgagcc
     5101 ggcgagcgac ctcgtcgcct cccggctggg cctgccgccc cgcgctcccg tgacgcaggt
     5161 gcgccgcgta cgccatctcg accgccagcc cgtgtcgctc gacatcacct gggtgccgcg
     5221 cgagctcggc cagcgcctgg cccgcgagga tctggccagg cgcgacatct tcctgatcct
     5281 cgagaacgac tgcgcgacgc cgctcggcca cgccgacctc gtgatcgacg ccggcctggc
     5341 cgacgcggcg ctcgccgacg ccctcgagat cagtcccggc gccccgatgc tgcgcatcga
     5401 gcgcctcacc cacgacacgg ccggccggcc cgtcgactac gaacacctct actgccgcgc
     5461 ggacaacttc cagtaccgcc tgcgcatcga gcggcgctga ggccgcaagg gaccccgatg
     5521 gacaccttca cccacgaagt cgacgtgctg gtcatcggcg gcggcaccgc cggcccgatg
     5581 gcggccgtca aggccaggca ggccgacccg tcgctgcgcg tgctgctgct cgagaaagcc
     5641 aacgtcaagc gcagcggcgc gatctcgatg ggcatggacg gcttgaacaa cgccgtcgtg
     5701 cctgggttcg ccacgcggga gcaatacgtg cgcgagatca ccgccgccaa cgacggcatc
     5761 gtcaaccaga agacgctgat ggcctacgcg cagggcagct tcgacatgat cgaggagctc
     5821 gatcgctggg gcgtcaagtt cgagaaggac gagaccggcg actatgcgat gcgcaaggtg
     5881 caccacctgg gcacctacgt gctgccgatg cccgaggggc acgacatcaa gaaggtgctg
     5941 taccgccagc tcaagcgcgc gcgggtggag atcaccaacc gcctcgtggc gacgcgcctg
     6001 ctcacggacg accagggccg catcgccggc gcgatggcct tcgattgccg cacggccgac
     6061 ttccacgtga tccgcgcgaa gtccgtcgtg ctgtgcacag gcgcggcggg ccgcctgggc
     6121 ctgccggcct ccggctacct gttcggcacc tacgagaacc cgaccaacgc gggcgacggc
     6181 tacagcatgg cgtaccacgc cggcgccgag ctctcgggca tcgagtgctt ccagatcaac
     6241 ccgctgatca aggactacaa cggtccggcc tgcgcctacg tcaccggccc gttcggcggc
     6301 tacacgacca acgcgcgcgg cgatcgcttc atcgagtgcg actactggtc gggccagatg
     6361 atgtgggagt tccagcagga actgcagggc ggcaggggcc cggtgttcct caagctggat
     6421 cacctggccg aggagacgat cgcgcagatc gagaccatcc tgcacaccaa cgagcgcccg
     6481 agccgcggcc gtttccacgc gggacgcggc acggactacc gcgagcgcat ggtggagatg
     6541 cacatctcgg agatcggcct gtgcagcggc cattccgcct cgggcgtgtg ggtcgacgag
     6601 cacgcacgca cgacggtgcc cggcctgcac gccgcgggcg acctcgcctg cgtgccgcac
     6661 aactacatgc tgggcgcttt cgtctacggc cggctcgccg gcgagagcag cgcggcgttc
     6721 tgcgcggcgc acgacttcgc gccggtcgac gccggacagg tcgagcgcga gcgcgcgcgc
     6781 gtgtgggcgc ccctgcggcg cgagcgcggg ctgccgccca accaggtcga atacaagctg
     6841 cgccgcatgg tcaacgacta cctgcagccg cccaaggtga cgcgcaagat ggagatcggc
     6901 ctggagcgct tcgagcagat ccgcgaggac ctcgggcacc tgcacgcgcg cgacccgcac
     6961 cagctgatgc gcgcgatgga ggtgcacttc atccgagact gcgcggagat ggcggcgcgc
     7021 gcgtcgctgt tccgcaccga gagccgctgg ggcctgtacc acgcccgtgt cgacttcccc
     7081 gcacgcgacg atgcgcagtg gttctgccac gcccagatcc gcaagggcga cgacggcgcg
     7141 atgaccagct tcaagcgccc cctcgaaccc tacatcgtgc ccatggccga gaccgagcgc
     7201 gcgagctacc agcgcctgcg catcccggcc gcgcgcgccg acagcgacgc cgcgcccgcc
     7261 ctcgcctgac cctcccgccg aaagaacctt catgcactcc ttcgctccga tcgcgctgca
     7321 cccggccagc gtccccgtca gcgtcgaccc cgacaagtgc atcgcccaca agggctgcac
     7381 cgtgtgcgtg gacgtgtgtc cgctcgacgt gctcgccatc gacctcgtca agggcagcgc
     7441 ctacatgaag tacgacgagt gctggtactg catgccctgc gagaaggact gccccaccgg
     7501 cgccgtccac gtcgacatcc cctacctggt tcgctgacat ccaccggaga cccttcgtga
     7561 agatcccatc cttcctggcc ggcgcgctgc tggccaccgc cctcgccttc gcgcccgcgg
     7621 ccttcgccgg cgagaccatc cgcgtggcca tcggcacgca ggacacgacg atcaactgcg
     7681 ccaccggcgg cctgctggtg cgcgagctgc acctgctcga gaagtacctg ccgcacgacg
     7741 gccgctacaa ggacgtgcag tacgacatcg agtggcacaa cttcacctcg ggcgcgccgc
     7801 tgaccaacga gatggtggcc gacaagctcg acttcggcgc gatggccgac ttccccggct
     7861 cgctcaacgg cgccgcgttc ctcaaggcgg gcaggaagag cctgttcctg tcggtgctct
     7921 cgggctcgac gatcggcagc ggcaacggca tcgtggtgcc cgtgaaatcg ccggtgcagt
     7981 cgctcgccga cctgaagggc aagcagattt ccgtgccgtt cgcgtccacg tcgcatggca
     8041 tgctgctgcg cgcgatcaag gcgcagggct gggatccgga gcgcgacgtg acgatcgtca
     8101 cgcaggcgcc cgaggtcgcc ggctcggcgc tgcaggccgg caagatcgac gcgcacgccg
     8161 acttcgtccc gttcgccgac ctgttcccgt acaaaggctt cgcgcgcaag atctacgacg
     8221 gcgcgcaggc cgacgcaccc accttccacg gcacgctggt gagcgcggac tatgcgaaga
     8281 agtatcccga gatcgtcgtc gcctacctgc gcgcggtgat cgaggcgaac cggctgttcg
     8341 acgccgatcc gctcaaatac agcgagctcg tcgccaaggt cacgggcatc gacgccgagg
     8401 tcgcgtacct gttcggcggc ccgctgggcc tgcagacgcg cgacctgagc tggaagcccg
     8461 agtaccgcca ggcgctggcc acggccatcg acacgctgcg cctgctcaag cgccacgact
     8521 ccgcgctgac cgccgacacc ttcatcgacg attcgtacct gcgcgccgcg ttcaaggccg
     8581 aggggctgga ttacgacgcg cagttgaaga actacgcgcg cctgccgctg aaatcgagcg
     8641 acgcgctgac cggcaagccc atcgccgacg acacgcgcgc cgcgcaggtc tgggtggcgg
     8701 gcgagccgcg cgtgcgcagc tacgcctcgc cggagaacgc gttcgccgac gcccggaagg
     8761 tcgaggccgc cggcggcaag gtgcgcgtcg tctacgcgca ggatcgcagg ctgggcatca
     8821 agctgctggc cgaccaggcc tggttcgccg ccgacgacaa gggccgcatc agcgccttcc
     8881 tgctcaagga cgacgcgacg cgttgggcgc gcgacaacca cgcccgggtg ctggacttcg
     8941 ccgccgcgcg cggcttcgcg gcgacgcacg tcgcgtccgc gcgctgacgc cgggacggcc
     9001 atgagcgcgg tgctcagccc cgacgtcgac gcgccgccgg cgcgcccgcc cgccgccgcg
     9061 gcgctgcgcc ggcgcggcgc gggctggctc gcgcagggcg cggccatcgt cgccttcgtg
     9121 gcgctgtggc acgcgctggc cgccacgcat gcgcacctgg gattcgtcga cttccgcaac
     9181 gtgccgaccc ccgtggccgc gctcgacgcc ggacgcgacc tcgcccacag cggcaagctc
     9241 gcgcgccacc tcggctacag cctctggcgc gtcggcgcgg gcttcgcctc ggcggccgtg
     9301 ctgggcatcg cgctggggct ggcgatcggc cgcttcgcgg tcgtgcggcg cgcgctgctg
     9361 ccgccgctgg agctgctgcg tccgatcccc gccgtcgcgt ggatcccgct ggccatcctg
     9421 atgttcccgt cggccgagct ctcgatggtg ttcatcacgt tcatcggcgc actgttcccg
     9481 gtgctgctca acaccctgca cggggtcgag tccgtcgacc ggcgcctggt cgcctccgcg
     9541 cgcagcctcg gcacgggacg ctggtcgctg ttcgcgcagg tgatccttcc ggccgcggcg
     9601 cccagcatcg ccacgggcct ggcgatcggc atgggcacct gctggttctg cctcgtgtcg
     9661 gccgagatga tcgcgggcca gttcggcatc ggctactaca cgtgggagtc ctacacgctg
     9721 cagaactacg gcggcatcat cgtgggcatg ctcgtgatcg gcctgctggg catgggctgc
     9781 agcgcactca tccgctggac ggcgcgactc gcgatgccgt ggcggcgacc ggaggacgcg
     9841 cgatgaacgc caccgtcggc cgcatcgaca tcgagcacgt gcacatggag gtcggcgacg
     9901 gcccggcccg cttcgaggcg ctgcacgacg tgagcctcgc catcgagccc ggcgagttcg
     9961 tctgcctgct cgggccctcg ggctgcggca agtccacgct gctgggtgcg ctcgcgggcc
    10021 acctggcccc cgcgcgcggc acgctgcgcg tggacggcgc gcgcgtcgac ggcccgcatc
    10081 ccgaccgcgg cctcgtgttc cagcagcaca ccctgtttcc gtggctgcgc gtcatcgaca
    10141 acgtggccta cggcctgaag atgaaaggcg tcgcgcgcgc ggcgcgtcat gcgcgggcgc
    10201 acgcgctgct ggcgagcgtg ggcctggcgg gcttcgagca gcgctggccg tcgcagctgt
    10261 cgggcggcat gcagcagcgc gtggagatcg cgcgcgtgct gatcaacgag ccgcgcgtgc
    10321 tgctgatgga cgagccgttc ggcgcgctcg acgcgctcac gcgttcgcgc atgcagcagc
    10381 tgctgctgga cgtctggacc cggttccgca ccaccgtcgt cttcgtcacg cacgacatcg
    10441 acgaggcgct gttcctcgcc gaccgcctgc tggtgatgag cgcgcggccc ggccgcatcg
    10501 tcgacgacct gcgccttcaa ttcccgcgcc cgcgcgagcc cgcgatcctg acctcgcccg
    10561 acttcgccgg gctcaagcgc cactgcctcg agctgctgca tcgcggcgag tccgacgcgc
    10621 cgctcccgcg catcaccccg ctcggcccgc cgctggccgc ctccacgaac ccgtgacccg
    10681 atccatgagc ttcgaacccg tgaacgaccc gcgcctcgcc agcgacgacc ccggcgtgcg
    10741 ccgcatcgcc gtcctggaca tcgccgacgc cgaggacgac gccgacctgc cggccctgat
    10801 caccgcgctg ctcgcggacc aggccgcgca ggtacgtgcc gaggccgcgc gcgtcctggg
    10861 cggctggggc catccgctgg ccgtcgacgc gctcgcgcag gccctgctcg acgccgacgc
    10921 cggcgtgcgc gcgaacgccg ccaccgccct cgcgcaactg aaggacgcgg ccgccggcga
    10981 gtgcctgctg ccctgggccc tgcacgacga cccggccgtg cgcgccgcgg cgctgcgcgg
    11041 actcaaggag ctgcgcatcc ccgcggcggc ggcgccggcg cgcgccgcac tcggccaccg
    11101 cgacgcgggt gtgcggcgcg aggccgtggg cgtgctgggc tggctgaagt
//
LOCUS       DATCRO010000062         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_484575, whole genome shotgun sequence.
ACCESSION   DATCRO010000062 DATCRO010000000
VERSION     DATCRO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484575"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VIN75_01705"
     CDS             complement(<1..1298)
                     /locus_tag="VIN75_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8972903.1"
                     /translation="MDFAYSPRSLALQEQLRAFMAAHVVPAEPRFHAEIEANTAAGRR
                     WTPLQLIEELKAKARAEGLWNLFLPDSATAQSARPLRSDAASPTGAASGASGANMYGA
                     GLSNQDYAPLAEIMGRTPWASEVFNCSAPDTGNMEVLVRYGTTEHQQRWLEPLLDGRI
                     RSAFAMTEPAVASSDATNIESRIVRDGDDYVLSGRKWWTSGAGDPRCAVYIFMGKTDP
                     EAPPHSQQSMILVPADAPGLKVLRPLTVFGYDDAPHGHMEVAFDDVRVPAANMLLGEG
                     RGFEIAQGRLGPGRIHHCMRLIGLAERALELMCERAAGRVAFGKPIAAQTVTQERIAE
                     ARCQIDMARLLTLKAAWMMDVAGNKTAKAEIAMIKVVAPNMACRVIDWAMQAFGAMGM
                     SEDAPLAYFFTVARTLRFADGPDEVHRNAIAKIELGKLRKA"
     gene            complement(1300..2394)
                     /locus_tag="VIN75_01710"
     CDS             complement(1300..2394)
                     /locus_tag="VIN75_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8972904.1"
                     /translation="MNQTEQNTGTRPVAESHRFDIAALESFLSKNLAGFAGPLSVEQF
                     KGGQSNPTYKLVTPSRSYVMRSKPGPVAKLLPSAHAVEREYRVMDALARTDVPVAKMH
                     LLCEDESVIGRAFYVMEYVEGRVLWDQGLPGMTQAGRGAIYDEMNRVIAAMHNVDVDA
                     IGLADYGKPGNFFARQIGRWTKQYQASITEPIEAMDRLIEWLPGHMPASAMDESQVSI
                     VHGDFRMDNLIFHPTEPRVLAVLDWELSTLGHPLGDFAYHCMAWHIPPGTFRGIAGLD
                     HAALGIPSERDYVRRYCERTGRTDPQALLRDWNFYLAYNLFRLAAILQGIARRVVDGT
                     ASSAQARATGAGARPLAELAWSFAQKSQEA"
     gene            complement(2462..3166)
                     /locus_tag="VIN75_01715"
     CDS             complement(2462..3166)
                     /locus_tag="VIN75_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8972905.1"
                     /translation="MNNPILTIEEQNNIDAGPWFSRLSDELRAAILSRAVVRRLPDGA
                     PLASRGSPAEDWCGVAKGAVRVSSVSLSGKQVSLTYVEPGTWFGDIALFDGLPRTHDA
                     DAHGATTLLSVRKADFKELLQQHVELYDALLRLNCRRLRIMFNLVEDLNTRPLSARLA
                     KNILMLAKSYGINQGDEVRIGLQLAQEDLAQLVGASRQRVNQELKELEREGALRIEPT
                     RLVVLSREKLVQAAEH"
     gene            3243..>3387
                     /locus_tag="VIN75_01720"
     CDS             3243..>3387
                     /locus_tag="VIN75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8972906.1"
                     /translation="MSPARRRLYLMRHGSVDYFKADGTPVPPHTVPLNEAGRAQADAA
                     GALF"
BASE COUNT          473 a         1181 c         1173 g          560 t
ORIGIN      
        1 gccttgcgca gcttgcccag ctcgatcttg gcgatcgcgt tgcggtgcac ttcgtcgggg
       61 ccgtccgcga agcgcagggt acgggccacg gtaaagaagt acgcgagcgg cgcgtcctcg
      121 gacatgccca tcgcgccgaa ggcctgcatg gcccagtcga tcacgcggca ggccatgttc
      181 ggcgccacca ccttgatcat cgcgatctcg gccttggccg tcttgttgcc ggccacgtcc
      241 atcatccacg cggccttgag cgtgagcagg cgcgccatgt cgatctggca gcgcgcctcg
      301 gcgatgcgct cctgcgtcac cgtctgcgcg gcgatcggct tgccgaacgc gacacgaccg
      361 gccgcgcgct cgcacatcag ctccagcgcg cgctcggcca ggccgatcag gcgcatgcag
      421 tggtggatgc ggcccgggcc gaggcggccc tgggcgatct cgaagccgcg gccttcgccc
      481 agcagcatgt tggccgcggg gacgcgcacg tcgtcgaacg cgacctccat gtggccgtgc
      541 ggcgcatcgt cgtagccgaa caccgtcagc ggccgcagca ccttcagccc cggcgcgtcg
      601 gcgggcacga ggatcatcga ctgctgcgaa tgcggcggcg cctcggggtc cgtcttgccc
      661 atgaagatgt agacggcgca gcgcggatcg ccggcgcccg acgtccacca cttgcggccg
      721 ctcagcacgt agtcgtcgcc gtcgcgcacg atgcgcgact cgatgttggt ggcgtccgac
      781 gaggccaccg ccggctcggt catcgcgaaa gcgctgcgga tgcggccgtc cagcagcggc
      841 tccagccagc gctgctggtg ctccgtcgtg ccgtagcgca ccagcacctc catgttgccg
      901 gtgtcgggcg ccgagcagtt gaagacctcg ctggcccagg gcgtgcggcc catgatctcg
      961 gccagcggcg cgtagtcctg gttcgacagg ccggcgccat acatgttggc cccgctcgct
     1021 ccgctcgccg cccccgtggg gctcgccgca tcgctccgga gcggccgcgc gctctgcgcc
     1081 gtggcggagt cgggcaggaa caggttccac aggccctcgg cgcgcgcctt ggccttgagt
     1141 tcctcgatca gctgcagcgg cgtccagcgc ctgccggcgg cggtgttggc ctcgatctcc
     1201 gcgtggaagc gcggctcggc gggcacgaca tgggccgcca tgaaggcgcg cagttgttcc
     1261 tgcagcgcca ggctgcgggg cgagtaggcg aagtccatgt caggcctctt gggatttttg
     1321 agcgaacgac caggcgagct ccgcgagcgg gcgcgcgccg gcgcccgtcg cgcgcgcctg
     1381 cgcgctcgag gccgtgccgt cgacgacgcg gcgcgcgatg ccctgcagga tggccgcgag
     1441 gcggaacagg ttgtaggcga ggtagaagtt ccagtcgcgc agcagcgctt gcgggtcggt
     1501 gcggccggtg cgctcgcagt agcggcgcac gtagtcgcgc tccgacggga tgcccagcgc
     1561 cgcatggtcg aggccggcga tacccctgaa cgtacccggc gggatgtgcc aggccatgca
     1621 gtggtaggcg aagtcgccca gcggatggcc gagggtcgac agctcccaat cgagcaccgc
     1681 gagcacgcgc ggctcggtcg ggtggaagat gaggttgtcc atgcggaagt cgccgtgcac
     1741 gatggacacc tgcgactcgt ccatcgcgct ggcgggcatg tgcccgggca gccactcgat
     1801 gagccggtcc atcgcctcga tgggttcggt gatcgaggcc tggtactgct tggtccagcg
     1861 gccgatctgg cgcgcgaaga agttgccggg cttgccgtag tcggccaggc cgatggcgtc
     1921 gacgtcgacg ttgtgcatcg ccgcgatcac gcggttcatc tcgtcgtaga tcgcgccgcg
     1981 cccggcctgc gtcatgcctg gcaggccctg gtcccacagc acgcggcctt cgacgtactc
     2041 catcacgtag aacgcgcggc cgatgacgga ctcgtcctcg cacagcaggt gcatcttcgc
     2101 gacgggcacg tccgtcctgg cgagcgcgtc catcacgcgg tactcgcgct ccaccgcatg
     2161 cgccgagggc agcagcttcg cgacgggccc gggcttggag cgcatcacgt acgagcgcga
     2221 cggcgtgacg agcttgtagg tcgggttgga ttggccgccc ttgaactgct cgacgctcag
     2281 cggccccgcg aagcccgcga ggttcttcga gaggaaggat tcgagcgccg cgatgtcgaa
     2341 gcggtggctc tcggcgaccg gccgggtgcc ggtgttctgt tcggtctggt tcatcggact
     2401 cgtctcgttg cctcaatgtc gatcatctcg ttcgacgcat cggcgacgtg tccgatgcat
     2461 cctagtgttc ggcggcctgc accagcttct cgcgcgacag caccacgagg cgtgtcggct
     2521 cgatgcgcag cgcgccttcg cgctccagct ccttgagctc ctggttgacg cgctggcgcg
     2581 acgcgcccac cagctgcgcc aggtcttcct gcgccagctg caggccgatg cgcacctcgt
     2641 cgccctggtt gatgccgtag ctcttggcca gcatcaggat gttcttggcc agtcgcgcgg
     2701 acagcgggcg cgtgttcagg tcctcgacga ggttgaacat gatgcgcagc cggcggcagt
     2761 tcagccgcag cagcgcgtcg tacagctcca cgtgctgctg cagcagttcc ttgaagtcgg
     2821 ccttgcgcac cgacagcagc gtcgtcgcgc catgggcgtc cgcgtcgtgc gttcgcggca
     2881 ggccgtcgaa cagcgcgatg tcgccgaacc aggtgcccgg ctcgacgtag gtcagcgaca
     2941 cctgcttgcc cgacagcgac accgagctca cgcgcaccgc gcccttggcc acgccgcacc
     3001 agtcttccgc gggcgatccg cgcgacgcga gcggcgcgcc atccgggagc cggcgcacca
     3061 cggcgcgcga caggatcgcg gcccgcagtt cgtcggaaag tctggagaac catgggcccg
     3121 cgtcgatgtt gttctgctct tcgattgtaa gaatcgggtt gttcattcct tgtccctatc
     3181 gcgacagccg gcaggctttt gatctctcgc cgattgtcgt ctcaaacagg actcacacag
     3241 gaatgtcgcc cgcgcgccgc cgcctctacc tgatgcgcca cggctcggtc gactacttca
     3301 aggccgacgg cacgcccgtg ccgccgcaca cggtgccgct caacgaggcc gggcgcgccc
     3361 aggccgacgc cgccggcgcg ctgttcg
//
LOCUS       DATCRO010000063        10159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_990801, whole genome shotgun sequence.
ACCESSION   DATCRO010000063 DATCRO010000000
VERSION     DATCRO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990801"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VIN75_01725"
     CDS             complement(<1..320)
                     /locus_tag="VIN75_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEY8972907.1"
                     /translation="MSLIHPTAIVDPAAELDSTVSVGPYAVIGPHVRIGAGTKVGPHC
                     VIEGRTTIGRDNTFYQFGSIGAMPQDMSHKGELTELRIGDRNTIREFCTLNTGTHKEE
                     GVTRV"
     gene            complement(341..787)
                     /gene="fabZ"
                     /locus_tag="VIN75_01730"
     CDS             complement(341..787)
                     /gene="fabZ"
                     /locus_tag="VIN75_01730"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY8972908.1"
                     /translation="MMDIHQIIKKLPHRYPFLLVDRVVEFEKDARIKALKNVTINEPF
                     FNGHFPNRPVMPGVLMLEALAQASALLSFASEGETSDGSRLYYFAGIDGARFKRVVEP
                     GDQLILESTITRKKGAIYKYATRALVDGELAVEAELMCTVRAVEAV"
     gene            complement(798..1832)
                     /gene="lpxD"
                     /locus_tag="VIN75_01735"
     CDS             complement(798..1832)
                     /gene="lpxD"
                     /locus_tag="VIN75_01735"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY8972909.1"
                     /translation="MNSVRLGEIVAALGGELRGDPAHLIARIAPLETAGGDAITFVAQ
                     ARLQKALEASAAGAIVVAPSLVAAAPTGRSLVVTDDPYLYFARLTQWWARRLRPAAAA
                     GVHPTAWVAPDAVVAASATIGPHAVVEAGAVIGERVQLGAHCVVGAGSKVGADSLMHP
                     RVTIAAGCTVGERCVFQSGAVIGADGFGIAPTQGRWERIEQLGGVRIGNDVDIGANTC
                     VDRGALEDTVLEDGVKLDNQIQIGHNCHIGAHTVMAACVGIAGSTKVGRNVMIGGAAM
                     IQGHIEIADGVTVSASTMVMHSLRKKGVYTAIFPVDEHASWEKNAVTLRNLYALRERV
                     RALEKASKKD"
     gene            complement(1852..2355)
                     /locus_tag="VIN75_01740"
     CDS             complement(1852..2355)
                     /locus_tag="VIN75_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY8972910.1"
                     /translation="MKLIYALLLASFSSVAVSASAQELKIGYVNSERVVRESDIAKRS
                     QAKLEAEFSKREKDLNDQNSALRAQAEKLDRDGPTLSETEKTRRQRELVELDRDLQRK
                     HREFQEDLTQRKNEELSSIAERANRTIKQIADAEHYDLIVQDPVIYASPRVDLTKKVI
                     DALNAGK"
     gene            complement(2370..4652)
                     /gene="bamA"
                     /locus_tag="VIN75_01745"
     CDS             complement(2370..4652)
                     /gene="bamA"
                     /locus_tag="VIN75_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY8972911.1"
                     /translation="MRPVAACVILAGATLPFAALAIEPFQIRDIRVEGLQRTDPGSVF
                     SVLPFRVGDSYSDEKAAAALRALFATGLFKDARIDVEGDVVVVIVDERATIASVNFLG
                     TKEFDKDTLTKALKDAGIGEGLPFDKAIVDRAEQEIKRQYLGHSLYAAEVVTTVTPLE
                     RNRVNVTFKVSEGDVAHIRDIRIIGAKAFSESKLIDQMDLTTGGWLTWYTKSDRYSRT
                     KLNADLEKLRAFYLNKGYLEFDITGTQVTISPDKQTISVTITISEGQPYIVTGVRLEG
                     NYLGREAQFKNLINVKPGANYHGDDVTTTVKAFTDLFGTYGYAFAHVEPQPQIDRKTG
                     QVVLVLAADPGRRAYVRRVNLSGNTITRDEVIRREFRQFESAWYDGSKIKLSRDRVDR
                     LGFFSSVDVDTNDVPGAPDQVDLTVNVVEKPTGNLTLAAGYSQADKISLTGSIKKENV
                     FGTGNYISLDVSTAHTNKNLSISETNPYFTDDGISRTFDIYYRTSRPLNSSGDAYAVS
                     TPGADIRFGLPYSEFDTVYVGVGAEQTHIGTLQGVPISYYRFVGEFGSFANSFPITAG
                     WARDERDSGVSPTSGRYMRLNSDFSLFGDVQYARLNGQVTQYVDLGNHFSLGINAEVG
                     YGTGLNGRSYPVFKNFYGGGLGSVRVFEQGSLGQGTVDATGAFIGGSKKFNFNNELYI
                     PVPGADKDKSLRLFAFFDAGNVWTEHNHMDFGSLRASAGLGLSWTSPVGPFKLSYGLP
                     VRAEPNDRIQRFQFQVGTAF"
     gene            complement(4684..6051)
                     /gene="rseP"
                     /locus_tag="VIN75_01750"
     CDS             complement(4684..6051)
                     /gene="rseP"
                     /locus_tag="VIN75_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY8972912.1"
                     /translation="MATLQTLFWFLVTLAILIPVHEYGHYRVAKACGVKVLRFSIGFG
                     NVIWSRRFGPDQTEFALSMIPLGGYVKMLDEREGAVDPAELPRCFNQRPLYQRALVVA
                     AGPFANLALAIALYSFVQWYGVDMPTPVVGAPAPGSVMADAGLQRDDRIIATAQGADA
                     GDDDFRAVRSWDDIIRAVNGALVEHEPLQLRVVQEGHANVRTLTAAVDGIDPFSAPRP
                     GSKALGLAPVARIGTVLPDGAAASSGLRAGDLVLRIDGKPVDDAVAVRLAIEASGATG
                     TPRTMRWDVDRAGQTLTIPVTARMEATDGTPVARAGIGFQHAVVRAPAVEALGLGAEQ
                     TWREAKSSWRMFGLILTGKVSTKAIGGAPTIAVAANAAAHQGFAQFVGFLAMVSLALA
                     VLNLLPIPLLDGGMLLYYLFEGATGRPVSELWQLWLQRGGALILLLLMTLALSNDAVR
                     FLGLQ"
     gene            complement(6051..7256)
                     /gene="ispC"
                     /locus_tag="VIN75_01755"
     CDS             complement(6051..7256)
                     /gene="ispC"
                     /locus_tag="VIN75_01755"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8972913.1"
                     /translation="MTSTSRRQRVCILGSTGSIGTSTLDVIALHPDTFEVFALTGQRR
                     TDDLFAQIVRWRPRFAALPDAAAANELGARVKAAGIATEVLAGAKALEELASHPDVDQ
                     VMAAIVGAAGLPACLAAARAGKRLLLANKEALVVGGQLFVDAVRQGGATLIPIDSEHS
                     AIFQCLPTDQADWDAAVDHIVLTASGGPFRTRDPATLEAVTPDQACAHPNWVMGRKIS
                     VDSATMMNKALEVIEARWLFDLAPERIKVVIHPQSIIHSMVVARDGAVLAQLGTPDMK
                     GPIALGMSWPRRMTSGCDALDFTKMAALTFEEADERRFPGLHLSWAALRAAPGSTTVL
                     NAANEIAVEAFLAGRLGFTGIHRVNAQALEQVLPAAGDAHALDALLALDARARDAARA
                     HVARLGKGA"
     gene            complement(7265..8101)
                     /locus_tag="VIN75_01760"
     CDS             complement(7265..8101)
                     /locus_tag="VIN75_01760"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8972914.1"
                     /translation="MLRERVITALVLLALLIPAVMARSPLPFALLTIVLIAAGGWEWG
                     RLCALPKWASIASGVVLGLACVAIAPLGSGIGHVAIPAELWAAVTALWVLGGALALRA
                     GPEGFRKVSRGLRFLVGWILIYAAWAALMASWERGLAYLATVCALVWAADIFAYFGGR
                     ALGKRKLAPTISPGKSWEGAISGLVSVIVLALVVVAVTVHPHGNLFWLLRDRLGYPGL
                     VAALVALVALAIVGDLVESLAKRAAGIKDSSQLLPGHGGVLDRIDALLPVFPAALALV
                     TL"
     gene            complement(8091..8861)
                     /gene="uppS"
                     /locus_tag="VIN75_01765"
     CDS             complement(8091..8861)
                     /gene="uppS"
                     /locus_tag="VIN75_01765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8972915.1"
                     /translation="MTPPASTFGASTRDPNVPRHVAIVMDGNGRWARKRFMPRIFGHK
                     HGVDALVATINACANRGVEHLTVFAFSSENWKRPEEEVSGLMGLVLSAMTRYLTKIAG
                     DGVRVKIIGDRAAVSQTVRNAWERAESTTAHNTRITVWVAFNYGGRWDIVQACRRAIA
                     DGVPADQLTEERLSRYMAQHEAPDPDLFIRTGGEVRISNFLLWQVAYSELYFSDCLWP
                     DFDDAELDRAFKVYASRDRRFGGVKPEAAALAGSPDAA"
     gene            complement(8870..9430)
                     /gene="frr"
                     /locus_tag="VIN75_01770"
     CDS             complement(8870..9430)
                     /gene="frr"
                     /locus_tag="VIN75_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8972916.1"
                     /translation="MSTADIKNTAQTKMGRSIEAFKAELQKIRTGRAHPGMLDQIHVE
                     YYGSPVPLSQVAQLSLLDARTIGVQPWEKPMISKVEKAIRESDLGLNPSTTGDIIRVP
                     MPALTEERRKDLTKVVKGLGEDAKVAVRNLRREANEHGKKLLKDKEITEDDERRLMDE
                     IQKLTDRTIAEVDHLVQSKEQEILAV"
     gene            complement(9496..10158)
                     /gene="pyrH"
                     /locus_tag="VIN75_01775"
     CDS             complement(9496..10158)
                     /gene="pyrH"
                     /locus_tag="VIN75_01775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8972917.1"
                     /translation="MGDDAYGINRATIVRMVQEVQEVTRLGTEVAVVIGGGNIFRGVA
                     GGSVGMDRATADYMGMLATVMNSLALADTMRQEGMVARVMSAISIEQVVEPYVRPKAL
                     QYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEMGAEIVLKATKVDGVYTADPKKVPDA
                     TRYQSITFDEAIGKNLQVMDATAFALCRDQNLPIKVFSIFKPGALKRVVLGEDEGTLV
                     HV"
BASE COUNT         1509 a         3403 c         3594 g         1653 t
ORIGIN      
        1 acgcgcgtga cgccctcttc cttgtgcgtg cccgtgttga gcgtgcagaa ctcgcggatc
       61 gtgttgcggt cgccgatgcg cagctcggtg agctcgccct tgtggctcat gtcctgcggc
      121 atcgcgccga tggagccgaa ctggtagaac gtgttgtcgc ggccgatcgt ggtgcggccc
      181 tcgatcacgc agtgcgggcc caccttggtg ccggcgccga tgcgcacgtg cgggccgatg
      241 acggcgtacg ggccgacgct gacggtcgaa tcgagctcgg cggccgggtc gacgatcgcg
      301 gtcggatgga tcaggctcat cgcaagctcc cgatccgcgc tcagacggct tcgaccgctc
      361 ggaccgtgca catcagctcg gcctcgacgg ccagctcgcc gtcgaccagc gcacgggtcg
      421 cgtacttgta gatggcgccc ttcttgcggg tgatcgtgga ctcgaggatc agctggtcgc
      481 ccggctccac gacgcgcttg aagcgcgcgc cgtcgatgcc tgcgaagtag tacaggcgcg
      541 agccgtccga agtctcgccc tcggaggcga acgacagcag cgccgaggcc tgggccagcg
      601 cctccagcat cagcacgccg ggcatcacgg ggcggttcgg gaagtgcccg ttgaagaacg
      661 gctcgttgat ggtgacgttc ttgagcgcct tgatgcgcgc gtccttctcg aactcgacga
      721 cgcggtcgac gagcaggaac gggtagcggt gcggcagctt cttgatgatc tggtggatgt
      781 ccatcagcat ggcggtctca gtctttcttt gatgccttct cgagcgcgcg gacgcgctcc
      841 cgcagggcat acaggttgcg cagcgtgact gcgttctttt cccagctggc gtgctcgtcg
      901 acggggaaga tcgccgtgta gacgcccttc ttgcgcagcg aatgcatcac catcgtcgag
      961 gccgacaccg tcacgccgtc ggcgatctcg atgtggccct ggatcatcgc ggcgccgccg
     1021 atcatgacgt tgcggccgac cttcgtcgag ccggcgatgc ccacgcaagc cgccatcacc
     1081 gtgtgcgcgc cgatgtggca gttgtggccg atctggatct ggttgtcgag cttgacgccg
     1141 tcctcgagca cggtgtcctc gagcgccccg cggtccacgc aggtgttggc gccgatgtcg
     1201 acgtcgttgc cgatgcgcac gccgcccagc tgctcgatgc gttcccagcg cccttgcgtc
     1261 ggcgcgatgc cgaagccgtc ggcgccgatg accgcgccgc tctggaagac gcagcgttcc
     1321 cccacggtgc agccggccgc gatcgtgacg cgcgggtgca tcaggctgtc ggcgccgacc
     1381 ttcgagccgg cgcccacgac gcaatgcgcg ccgagctgca cgcgctcgcc gatgacggcg
     1441 cccgcctcga cgaccgcatg cggcccgatc gtggcgctgg ccgcgacgac ggcatcgggc
     1501 gcgacccatg cggtcggatg cacgccggcg gccgccgccg ggcgcaggcg cctggcccac
     1561 cactgggtca ggcgcgcgaa gtaaaggtag gggtcgtcgg tgacgacgag gctgcggccg
     1621 gtgggcgcgg cggcgacgag cgagggagcg acgacgatgg cgccggccgc actcgcttcc
     1681 agcgccttct gcagccgggc ctgcgccacg aacgtgatcg catcgccgcc ggccgtctcc
     1741 agcggggcga tgcgtgcgat caggtgcgcg gggtcgcccc gcagttctcc gcccagcgcc
     1801 gcaacgatct cacccaggcg aactgagttc atgttgcggg gtcccggagg tctacttgcc
     1861 ggcgttcagc gcgtcgatga ccttcttcgt caggtcgacg cgcgggctgg cgtagatgac
     1921 cggatcctgg acgatcaggt catagtgctc cgcatcggcg atctgcttga tcgtgcggtt
     1981 ggcacgctcg gcgatgctgg acagttcctc gttcttgcgc tgcgtgaggt cttcctggaa
     2041 ctcgcggtgc ttgcgctgca ggtcgcggtc gagctcgacc agttcgcgct ggcggcgcgt
     2101 cttctcggtc tccgacaggg tcgggccgtc gcgatcgagc ttctcggcct gggcgcgcag
     2161 cgccgagttc tggtcgttca ggtccttctc gcgcttgctg aattcggctt ccagcttggc
     2221 ctgggagcgc ttggcgatgt cggattcgcg cacgacgcgt tcgctgttca cgtagccgat
     2281 cttcagttcc tgggcggacg cagacaccgc gaccgacgaa aaagaggcca gcagcagggc
     2341 gtaaatcaat ttcatggaac gtgctttcat caaaacgcgg taccgacttg gaactggaac
     2401 cgttgaattc tatcgtttgg ctcggcccgg acaggaaggc catagctcag cttgaaggga
     2461 cccaccgggg aagtccagct caggcccagg cccgccgagg cgcgcagcga accgaagtcc
     2521 atgtggttgt gctctgtcca gacgttgccg gcgtcgaaga acgcgaacag gcgcaagctc
     2581 ttgtccttgt cggcgccggg caccgggatg tagagctcgt tgttgaagtt gaacttcttg
     2641 gagccgccga tgaacgcgcc ggtggcgtcc accgtgccct ggcccaggga gccctgctcg
     2701 aacacgcgca ccgagccgag gccgccgccg tagaagttct tgaacaccgg gtagctgcgg
     2761 ccgttcaggc ccgtgccata gccgacctcg gcgttgatgc ccagcgagaa gtggttgccc
     2821 aggtcgacgt actgcgtcac ctggccgttc aggcgcgcgt actgcacgtc gccgaacagc
     2881 gagaagtcgg agttcaggcg catgtagcgc cccgaggtcg gcgagacgcc gctgtcgcgc
     2941 tcgtcgcgcg cccagcccgc cgtgatcggg aacgagttgg cgaacgagcc gaactcgccg
     3001 acgaagcggt agtagctgat gggcacgccc tgcagcgtgc cgatgtgcgt ctgctcggcg
     3061 ccgacgccca cgtacaccgt gtcgaattcg gagtacggca ggccgaagcg gatgtccgcg
     3121 cccggcgtcg acaccgcgta cgcgtcgccc gagctgttga gcggacgcga cgtgcggtag
     3181 tagatgtcga acgtgcgcga gatgccgtcg tcggtgaagt acgggttggt ctcgctgatc
     3241 gacaggttct tgttggtgtg ggccgtgctg acgtccagcg agatgtagtt gcccgtgccg
     3301 aacacgttct ccttcttgat ggagcccgtc agcgagatct tgtcggcctg cgagtagccg
     3361 gcggccagcg tcaggttgcc cgtgggcttc tcgacgacgt tgaccgtgag gtcgacctgg
     3421 tcgggcgcgc ccggcacgtc gttggtgtcg acatcgacgc tgctgaagaa gcccaggcgg
     3481 tcgacgcggt cgcgcgacag cttgatcttg gagccgtcgt accaggccga ttcgaactgg
     3541 cggaactcgc ggcggatgac ttcgtcgcgc gtgatcgtgt tgcccgacag gttgacgcgg
     3601 cgcacgtacg cgcgacggcc cgggtcggcg gccagcacca gcacgacctg gcccgtcttg
     3661 cggtcgatct gcggctgcgg ctcgacgtgc gcgaacgcgt agccataggt gccgaacagg
     3721 tcggtgaacg ccttgaccgt ggtcgtgacg tcgtcgccgt ggtagttggc gccgggcttg
     3781 acgttgatca ggttcttgaa ctgcgcctcg cggcccaggt agttgccttc gaggcgcacg
     3841 cccgtgacga tgtagggctg gccctcgctg atcgtgatgg tgacgctgat ggtctgcttg
     3901 tccggcgaga tcgtgacctg cgtgccggtg atgtcgaact cgaggtagcc cttgttcagg
     3961 tagaacgcgc gcagcttctc gaggtcggcg ttgagcttgg tgcgcgagta gcggtcggac
     4021 ttcgtgtacc acgtgagcca gccgcccgtg gtcaggtcca tctggtcgat cagcttggat
     4081 tcgctgaacg ccttggcgcc gatgatgcgg atgtcgcgga tgtgcgcgac gtcgccttcg
     4141 gacaccttga acgtgacgtt gacgcgattg cgctccagcg gcgtgacggt ggtgacgacc
     4201 tcggccgcgt acaggctgtg gcccaggtac tggcgcttga tctcctgctc cgcgcggtcg
     4261 acgatggcct tgtcgaacgg caggccctcg ccgatgccgg cgtccttgag cgccttggtc
     4321 agcgtgtcct tgtcgaactc cttggtgccc aggaagttga ccgacgcgat ggtggcgcgc
     4381 tcgtcgacga tcacgacgac gacgtcgcct tcgacgtcga tgcgcgcgtc cttgaacagg
     4441 cccgtggcga acagcgcgcg cagcgcggcg gcggccttct cgtccgagta gctgtcgccg
     4501 acgcggaacg gcagcaccga gaacacgctg ccggggtcgg tgcgctgcag gccttcgacg
     4561 cggatgtcgc gaatctggaa gggttcgatc gcgagggcgg cgaacggcaa cgtggcgccc
     4621 gcgaggatca cgcaggcagc gacagggcgc atcaagctcg ggaagagtga agacatcagg
     4681 tgtttattgc aggcccagga aacggaccgc gtcattggaa agggcgagag tcatcagcag
     4741 caggagtatc agagccccgc cgcgctgcag ccagagttgc cacagttcgg agaccgggcg
     4801 accggtcgca ccctcgaaga gataatacag cagcatcccc ccatcgagca gagggatcgg
     4861 cagcaggttc agcaccgcca gcgcgaggct gaccatggcc aggaagccca cgaactgcgc
     4921 gaagccctgg tgcgccgcgg cgttcgccgc cacggcgatg gtgggcgcgc cgccgatggc
     4981 cttggtggac accttgccgg tcaggatcag gccgaacatg cgccacgagg acttcgcctc
     5041 gcgccacgtc tgctcggcgc cgagtccgag cgcctcgact gccggcgcac gcaccaccgc
     5101 gtgctggaag ccgatgcccg cgcgcgccac cggcgtgcca tcggtggcct ccatgcgtgc
     5161 ggtcacgggg atggtgagcg tctggccggc gcgatccacg tcccagcgca tcgtgcgcgg
     5221 cgtaccggtg gccccgctgg cttcgatcgc gaggcggacg gcgaccgcat cgtcgaccgg
     5281 cttgccgtcg atgcgcagca cgaggtcgcc cgccctcagg ccggagctcg cagccgcgcc
     5341 gtccggcagt accgtgccga tgcgggccac cggcgccagg cccagcgcct tggagcccgg
     5401 cctgggcgcg gagaacggat cgatgccgtc gacggcggcc gtcaacgtgc gcacgttggc
     5461 atggccttcc tgcacgacgc gcaactgcag cggctcgtgc tcgaccagtg cgccgttgac
     5521 ggcgcggatg atgtcatccc acgagcgcac cgcgcggaaa tcgtcgtcgc ccgcgtcggc
     5581 gccctgcgcg gtggcgatga tccggtcgtc acgctgcagc ccggcatcgg ccatcacgct
     5641 gccgggggcg ggcgcaccga ccaccggggt gggcatgtcc acgccgtacc actgcacgaa
     5701 cgagtacaac gcgatcgcca gcgccaggtt ggcgaacggc ccggccgcga cgaccagcgc
     5761 gcgctggtac agcggccgct ggttgaagca gcgcggcagt tcggccggat cgacggcgcc
     5821 ctcgcgctcg tcgagcatct tcacgtagcc gcccagcggg atcatgctga gcgcgaactc
     5881 ggtctggtcg ggcccgaagc ggcggctcca gatgacgttg ccgaagccga tcgagaaccg
     5941 cagcaccttg acgccgcacg ccttggcgac gcggtagtgg ccgtactcgt ggaccgggat
     6001 caggatcgcc agcgtgacga ggaaccagaa cagcgtctgc agggtggcca tcacgcgccc
     6061 ttgcccagcc tggccacgtg cgcgcgcgcc gcgtcacgcg cgcgggcgtc gagcgcgagc
     6121 agcgcgtcga gcgcgtgcgc gtcgccggcc gccggcagca cctgctcgag cgcctgcgcg
     6181 ttgacgcggt ggatgcccgt gaagcccagc cggcccgcga ggaaggcctc gacggcgatc
     6241 tcgttggcgg cgttgagcac ggtggtcgaa cccggcgcgg cccgcagcgc ggcccacgac
     6301 aggtgcagcc cggggaagcg ccgttcgtcg gcctcctcga aggtgagcgc ggccatcttc
     6361 gtgaagtcga gggcatcgca gccggaggtc atgcgacgcg gccacgacat gccgagcgcg
     6421 atcgggccct tcatgtcggg cgtgcccagc tgcgccagca cggcgccgtc gcgcgcgacc
     6481 accatcgagt ggatgatgct ctgcggatgg atcaccacct tgatgcgctc gggcgcgagg
     6541 tcgaacagcc agcgcgcctc gatcacctcg agcgccttgt tcatcatcgt ggccgagtcg
     6601 accgagatct tgcggcccat cacccagttc ggatgcgcgc aggcctggtc gggcgtgacc
     6661 gcctccagcg tggccgggtc gcgcgtgcgg aacgggccgc cggacgcggt caggacgatg
     6721 tggtcgaccg cggcatccca gtcggcctgg tcggtcggca ggcactggaa gatcgcggag
     6781 tgctcgctgt cgatcgggat gagcgtcgcc ccgccctgcc tgaccgcatc gacgaacagc
     6841 tggccgccga cgaccagcgc ctccttgttg gccagcagca ggcgcttgcc cgcgcgcgcg
     6901 gcggccaggc acgccggcag gcccgcggcg ccgacgatgg cggccatcac ctggtcgacg
     6961 tccggatgcg acgcgagctc ctcgagcgcc ttggcgccgg cgaggacttc ggtggcgatg
     7021 cccgccgcct tcacgcgcgc gcccagttcg ttggcggccg ccgcatcggg cagcgcggcg
     7081 aagcgcggac gccagcgcac gatctgcgcg aacaggtcgt ccgtgcggcg ctggccggtc
     7141 agcgcgaaca cctcgaaggt gtcggggtgc agcgcgatga cgtcgagcgt gctggtgccg
     7201 atcgagcccg tcgagccgag gatgcagacg cgctggcgcc gggaggtgga ggtcatcgcg
     7261 ggaatcagag ggtcaccagc gcgagcgcgg cggggaacac gggcagcagc gcgtcgatgc
     7321 ggtcgaggac gccgccgtgg ccgggcagca gctggctgga atccttgatg ccggccgcgc
     7381 gcttggccag cgattccacg aggtcgccga cgatggccaa cgccaccagc gcgacgagtg
     7441 cggcgaccag gccgggatag ccgagccggt cgcgcagcag ccagaacagg ttgccgtgcg
     7501 ggtgcacggt gacggccacc acgaccagcg ccagcacgat caccgagacg agcccgctga
     7561 tcgcgccctc ccagctcttg ccggggctga tggtcggcgc gagcttgcgc ttgccgagcg
     7621 cgcggccgcc gaagtacgcg aagatgtccg cggcccacac gagcgcgcag acggtggcga
     7681 ggtaggccag gccgcgctcc cacgaggcca tcagcgcggc ccaggcggcg tagatcagga
     7741 tccagccgac gagaaagcgc agcccgcggc tcaccttgcg aaagccctcg ggcccggcgc
     7801 gcagcgcaag cgcgccgccc agcacccaca gcgcggtgac ggcggcccag agctcggccg
     7861 ggatggcgac gtggccgatt cccgagccca gcggcgcgat ggccacgcag gccaggccca
     7921 gcacgacgcc cgaggcgatc gacgcccact tgggcagcgc gcacagccgg ccccactccc
     7981 agccgccggc ggcgatgagg acgatggtca gcagggcgaa cggcagcggc gaccgggcca
     8041 tcaccgccgg aatcagcagc gccagcagga ccagcgcggt gatgacgcgt tcacgcagca
     8101 tcggggctcc cggccagtgc ggccgcttcg ggtttgacgc cgccgaatcg gcggtcgcgc
     8161 gaggcgtaga ccttgaacgc acggtcgagt tccgcgtcgt cgaagtccgg ccacaggcaa
     8221 tcgctgaaat acagctcgga gtaggccacc tgccacagca ggaagttgct gatgcgcact
     8281 tccccgccgg tgcggatgaa caggtcggga tcgggcgcct cgtgctgcgc catgtagcgc
     8341 gacaggcgct cctcggtcag ctggtcggcc ggcacgccgt cggcgatggc gcgccggcag
     8401 gcctgcacga tgtcccagcg gccgccgtag ttgaaggcca cccacaccgt gatgcgcgtg
     8461 ttgtgcgccg tggtgctctc ggcgcgctcc cacgcgttgc gcaccgtctg cgacaccgcg
     8521 gcgcggtcgc cgatgatctt cacgcgcacg ccgtcgccgg cgatcttggt gaggtagcgc
     8581 gtcatcgcgc tgagcaccag gcccatcagg cccgagactt cctcttcggg ccgcttccag
     8641 ttctccgacg agaacgcgaa cacggtcagg tgctcgacgc cgcggttggc gcacgcgttg
     8701 atggtggcca ccagcgcgtc gacgccgtgc ttgtgcccga agatgcgcgg catgaagcgc
     8761 ttgcgcgccc agcggccgtt gccgtccatg acgatggcca cgtgccgcgg cacgttgggg
     8821 tcgcgcgtcg aagccccgaa ggtggaggcg ggcggagtca cggcgcgctt caaaccgcga
     8881 ggatctcttg ttccttggac tgcacgaggt ggtcgacctc ggcgatcgtg cggtcggtca
     8941 gcttctggat ctcgtccatc aggcgacgct cgtcgtcctc ggtgatctcc ttgtccttga
     9001 gcagcttctt gccgtgctcg ttcgcctcgc ggcgcaggtt gcgcacggcg accttggcgt
     9061 cctcgcccag gcccttgacg accttggtga ggtccttgcg gcgctcttcc gtcagcgcgg
     9121 gcatgggcac gcggatgatg tcgccggtgg tcgacgggtt caggcccagg tccgattcgc
     9181 ggatggcctt ctcgaccttc gagatcatcg gcttttccca cggctgcacg ccgatggtgc
     9241 gcgcgtcgag cagcgacagc tgcgccacct ggctcagcgg caccggcgag ccgtagtact
     9301 cgacgtggat ctggtccagc atgccggggt gcgcgcggcc ggtgcggatc ttctgcagtt
     9361 cggccttgaa cgcttcgatg gatcggccca tcttggtctg ggcggtgttc ttgatgtcgg
     9421 cggtactcat ggtctctcct cattggcacg cgcgccgcca tcggcgccgt gcgtcaacgc
     9481 atatccgggt cggcttcaga cgtgcaccag cgtgccttcg tcctcgccca gcacgacgcg
     9541 cttgagcgcg cccggcttga agatgctgaa caccttgatg ggcaggttct ggtcgcggca
     9601 cagcgcgaac gccgtggcgt ccatgacctg caggttcttg ccgatcgcct cgtcgaaggt
     9661 gatcgactgg tagcgcgtgg cgtcgggcac cttcttggga tcggccgtgt agacgccgtc
     9721 gaccttggtg gccttgagga cgatctcggc gcccatctcg gcaccgcgca acgcggcggc
     9781 ggtgtcggtg gtgaagaacg ggttgcccgt gccggccgcg aagacgacga ccttgccctc
     9841 ttcgaggtac tgcagcgcct tggggcgcac gtagggctcg acgacctgct cgatggagat
     9901 cgccgacatc acgcgcgcga ccatgccttc ctggcgcatc gtgtcggcca gcgcgagcga
     9961 gttcatcacc gtcgcgagca tgcccatgta gtcggccgtg gcgcggtcca tgccgaccga
    10021 gccgccggcc acgccgcgga agatgttgcc gccgccgatg accaccgcca cctcggtgcc
    10081 caggcgcgtg acttcctgca cctcctgcac catgcggacg atcgtggccc ggttgatgcc
    10141 gtaggcgtcg tcgcccatc
//
LOCUS       DATCRO010000064         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_574482, whole genome shotgun sequence.
ACCESSION   DATCRO010000064 DATCRO010000000
VERSION     DATCRO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574482"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VIN75_01780"
     CDS             <1..1213
                     /locus_tag="VIN75_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY8972918.1"
                     /translation="PLTLAAFQQVSPVGLLANLVAEPWVTLVVTPLTLLGIVLPPLWT
                     LAALALKPLLWWLGWLAHWPLASVHVATAPGWALAAGLAGGALLMLSLPWRVRGLGLA
                     MLPPLLWPFVARPPDGRFELVAADVGQGTAVLVRTRAHLLLFDTGPRLGDDNDAGRRL
                     LLPLLQARGEPHIDTLLLSHADADHVGGAQSLIDRMPVLALRSSLPAGNPLRANPIPH
                     TACVAGQAWRWDGVRFAILHPFPGDYRPGAKTNALSCVLRVVGADGRSALLTGDVEAA
                     GEARLVARARAAPGDAAASLRSDVLVVPHHGSRTSSTDAFLDAVRPDVAVIQVGYRSR
                     YGHPAPDVVARYRAHAIPVVRSDHCGAWTWSDGQARCTRDARRRYWQWQGAEQPLTPP
                     RAGRPAPPGEP"
     gene            1538..>2313
                     /locus_tag="VIN75_01785"
     CDS             1538..>2313
                     /locus_tag="VIN75_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY8972919.1"
                     /translation="MNTPFDEMHAAGDAVREHYQGYAQWLARQPADLMRDRRNEAELI
                     FRRVGITFAVYGDKDSGEGTERLIPFDQIPRVIPSHEWREMEKGLAQRVTALNRFIRD
                     VYHDQEILKAGVVPREQVLDNAQFRPEMIGVDVPGDVYSHIAGVDIVRAGNPDGSGSY
                     YVLEDNLRVPSGVSYMLENRKMMMRLFPELFSMHAVAPVAHYPDLLLETLRSLAPGGS
                     NDPTVVVLTPGMYNSAYFEHAFLAQQMGVELVEGQDLFVR"
BASE COUNT          281 a          876 c          824 g          332 t
ORIGIN      
        1 gccgctgacc ctggccgcgt tccagcaggt gtcgccggtg ggcctgctgg ccaacctggt
       61 ggccgagccc tgggtgacgc tcgtggtgac cccgctgacc ttgctgggca tcgtgctgcc
      121 gccgttgtgg acgctggctg cgctggcgct caagccgctg ctgtggtggc tgggctggct
      181 ggcgcactgg ccgctggcca gcgtgcacgt ggccaccgcg cccggctggg cgctggcggc
      241 cggcctggcc ggcggcgcgc tgctgatgct gtcgctgccc tggcgcgtgc gcgggctggg
      301 cctggcgatg ctgccgccgc tgctgtggcc cttcgtcgcg cggccgccgg acggccgctt
      361 cgagctcgtc gccgccgatg tcggccaggg caccgccgtg ctggtgcgca cccgcgcgca
      421 cctgctgctg ttcgacaccg gcccgcgcct gggcgacgac aacgacgccg gtcgtcgcct
      481 gctgctgccg ctgctgcagg cgcgcggcga gccgcacatc gacacgctgc tgctgagcca
      541 cgccgacgcc gaccatgtgg gcggcgcgca gtccctcatc gaccggatgc ccgtgctggc
      601 gctgcgctcc tcgctgccgg caggaaatcc gctgcgcgcc aacccgatcc cgcacaccgc
      661 ctgcgtggcc ggacaggcct ggcgctggga tggcgtgcgc ttcgcgatcc tgcatccgtt
      721 tcccggcgac tatcgccccg gcgcgaagac caacgcgctg agctgcgtgc tgcgcgtcgt
      781 cggcgcggac ggccgcagcg cgctgctgac gggcgacgtc gaggcggccg gcgaggcgcg
      841 gctcgtcgcc cgggcgcgcg ccgcgcccgg ggacgcggcg gcgagcctgc gcagcgacgt
      901 cctggtcgtt ccgcatcacg gcagccgcac ctcgtccacc gatgccttcc tcgacgcggt
      961 gcggcccgac gtggcggtga tccaggtcgg ctaccgcagc cgctacggcc accccgcgcc
     1021 cgacgtcgtc gcccgctacc gcgcccacgc catcccggtg gtgcgcagcg accactgcgg
     1081 cgcctggacc tggtcggacg ggcaggcccg ctgcacgcgc gacgcgcgcc gccgctactg
     1141 gcaatggcag ggtgccgagc agccgttgac gccgccgcgg gccggccggc ccgcgccgcc
     1201 cggcgagccg tgacgggcgg atcgctcggg atcggcctct gccatcgcgg cccttcgcga
     1261 cgacgtcttg acttcggcca gtcgcactcc gcgacgcccg ccgccgcgcc agcccaatcc
     1321 ggtgcgcccc gctgcgccat cctcgccccg cgttgcacag ctgcggtgcg tcgcggatac
     1381 tggggtcgcc aggccgctcg ccggactggc gtccgcggcg caccggcggc ctggtccgcc
     1441 ggtgcgcgcg gtcgtcgtat gctggatcgg ttgttgctat gcaatccaac ggaaacgccg
     1501 cgcggcgcgg caccgcacca gggagccctc gctcgccatg aacacgccat tcgacgaaat
     1561 gcacgccgcg ggggacgcgg tgcgcgagca ctaccagggc tacgcccagt ggctggcacg
     1621 ccagccggcc gacctcatgc gggatcgccg caacgaggcc gagctgatct tccggcgcgt
     1681 gggcatcacg ttcgcggtct acggcgacaa ggattccggc gagggcaccg agcggctgat
     1741 cccgttcgac cagatcccgc gcgtcatccc gtcgcacgag tggcgcgaga tggagaaggg
     1801 cctggcgcag cgcgtcacgg cgctcaaccg cttcatccgc gacgtctacc atgaccagga
     1861 gatcctgaag gccggcgtcg tgccgcggga gcaggtcctc gacaacgcgc agttccggcc
     1921 cgagatgatc ggcgtcgacg tccccggcga cgtctactcg cacatcgccg gcgtcgacat
     1981 cgtgcgcgcc ggcaaccccg acggctcggg cagctactac gtgctcgagg acaacctgcg
     2041 ggtgcccagc ggcgtgtcgt acatgctgga aaaccgcaag atgatgatgc ggctgtttcc
     2101 cgagctgttc tcgatgcacg cggtcgcgcc ggtggcgcac taccccgacc tgctgctcga
     2161 gacgctgcga agcctcgcgc cgggcggcag caacgacccg accgtggtcg tgctcacgcc
     2221 cggcatgtac aactcggcct acttcgagca cgccttcctc gcgcagcaga tgggcgtcga
     2281 gctggtcgag ggccaggacc tgttcgtccg cga
//
LOCUS       DATCRO010000065         6528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_698354, whole genome shotgun sequence.
ACCESSION   DATCRO010000065 DATCRO010000000
VERSION     DATCRO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698354"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIN75_01790"
     CDS             complement(<1..309)
                     /locus_tag="VIN75_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8972920.1"
                     /translation="MQYFVTGATGFIGKRLVKALLARRGATVFFLLRPGSESKVPDLL
                     AYWGAAKGRAVPVTGDLTGKRLGVAADQVKALKGQIDHVYHLAAVYDLDADEASQVAV
                     N"
     gene            545..1120
                     /locus_tag="VIN75_01795"
     CDS             545..1120
                     /locus_tag="VIN75_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8972921.1"
                     /translation="MVKKLSKIAAKKAASPAGLLDSQLASSVKESAQQIWLAGMGAFA
                     KAQAEGGKVFEALVKEGVSLQRKTQAVAEEKLGDVTGKMSSMASEVTSKAGASWDKLE
                     NIFEERTAKALGKLGVPTAKDVAELTRRVEELSAAVARLSKSGKAPAAGKTAAAKAPA
                     KKAAAKAPARKAAARRAPAKKAAPASAATAA"
     gene            1280..2605
                     /locus_tag="VIN75_01800"
     CDS             1280..2605
                     /locus_tag="VIN75_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8972922.1"
                     /translation="MPDTMSKTSDPAARPRARAPSRAPAAAPARTRRHGARAVGLALA
                     GGGPLGAIWEIGALCALEEALVGVDFTRMDGYVGISAGAFIAAGLANGMTPRQMCTAF
                     IENEGGDDDLVHPSIFVHPAWREFAGRLKRLPSLVAQSALRFALGQASGVEVIERLGR
                     VLPTGVFSNAPIEAHLRQVFSAPGRTDDFRALPRRLVLVATDLDTGAVAPFGLPGWDD
                     VPISRAVTASAALPGLYPPVRIGERHYVDGALKKTLHARALLDEDIGLVLCLNPLVPF
                     DATYAPTHTVRLGTDPIPRLVEGGLPLVASQSFRSLIHSRLELGLKGYGLSHPHVDIA
                     LFEPDHRDPEMFGANTFSYSARRQLAEHAYQSTRKTLRTRRRALHAMFEPHGIGLADG
                     VLDDPTRRLVTYTPRLGPAWHDVAPGRLGGALRRLGGVLDELESSLKPA"
     gene            complement(2592..3830)
                     /locus_tag="VIN75_01805"
     CDS             complement(2592..3830)
                     /locus_tag="VIN75_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972923.1"
                     /translation="MSTSNLYRGLFELLTLPHGDAAAEMLLTSGRVARRELLVVARQE
                     GCLPELYARWAAQSLLSPTETNEHEQIVRRRAGVADVLRALPPGSLLAASEAARSSAA
                     AIDVLLPDFAAIGPLHEALGRLEYRLGDAGQWHVPPRGPMHHGLAAFDYEPSLGSGLV
                     VRVQVGGAPIDATRHVAFADLAADAVRPNGLACKALAPTRQVLHRVAAFGARATPVTL
                     REIADLHQLLKDSAQPVDTTWLQVRIEQLDAWAGLRQLRDAIVAKRLGALLGWGAFGR
                     LVDASAAHAEATAGPQAGAPRVASFISGAFALVRGPRGNDIAARIARVPWLVSQVLGA
                     GHRVRGVPVSTKVFDAPRLMRIDGALYLATGAGVFLLSLVDLAEPARAELGQVVRTAS
                     RPVVLARWTAARGTRRTTRA"
     gene            complement(4021..4995)
                     /locus_tag="VIN75_01810"
     CDS             complement(4021..4995)
                     /locus_tag="VIN75_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8972924.1"
                     /translation="MKQLQGLLALIEIAAAGSFAGAARRLGVTPAAVSKSLARLEEQL
                     GVRLVQRSTRTARVTGEGERFIAKARDALRLLDDAVTEVSQAAREPAGVVRLSTGVAF
                     GRMWVLPALPALSARFPKLVLEVELDNAPVDLVANGIDIAIRGGSVPDAGFVARRVCK
                     LPLVLVASPDYLAHAGVPQSHAEFDRHRCIQLRFADGTAAPWTFRVNGRRVAVTPHAA
                     LVGTDSDAVVELALAGGGIAQCGLYQALPFLQAGRLKAVLADTHDAGTREFVLVYPHR
                     QFLAPRVRVVVEALLAHLRDTASLHMQPADLPAALRATAPGRRTRRVA"
     gene            5119..5754
                     /locus_tag="VIN75_01815"
     CDS             5119..5754
                     /locus_tag="VIN75_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8972925.1"
                     /translation="MKIGIIGSGVVAQTLAAGFIKHGHQVTLGTRDVSKLADFAVKTP
                     GARVAGSAEAAAFGEVVVLAVKGSAALQAARDAGAALDGKPVLDATNPIADAAPVDGV
                     LPYFTGKNESLMEWLQEAHPRARFVKAFNSVGAARMVDPDFGGTRPTMFIAGNDAGAK
                     DVATTLLAQLGWDTADMGTAVAARAIEPLAMLWCIPGFREGSWMHAFKLLR"
     gene            5792..>6528
                     /locus_tag="VIN75_01820"
     CDS             5792..>6528
                     /locus_tag="VIN75_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972926.1"
                     /translation="MAPRTWIVDSVGAATFERLASGLAGSELQSLLLDVMQARAASRS
                     AADLLAQHGRDAFCVPAQVDARAMLAIDARLFEAAEGFDAIELSPVAPLGACSVVGPT
                     DQNRVLSALRATEVVSDPTNVLALECAQRLRARPGVAHHLATSHRVMRAQPVPKLPGY
                     AQHFRLFALASGGVEREDHAFTVDILALHVRTMLAGLDRLVQQGFALERRRIEILATP
                     ARQPLGERLAKALDAELGALDHPYYSGG"
BASE COUNT          924 a         2439 c         2332 g          833 t
ORIGIN      
        1 gttgacggcg acctgcgacg cctcgtcggc gtccaggtcg tagaccgcgg ccaggtggta
       61 cacgtggtcg atctggccct tgagcgcctt gacctggtcg gccgcgacgc ccagccgctt
      121 gccggtgagg tcgcccgtca cgggcacggc gcggcccttg gcggcgcccc agtaggcgag
      181 caggtcgggc accttggact ccgaacccgg tcgcaacagg aagaacaccg tggccccgcg
      241 ccgggccagc agggccttga cgagtcgctt gccgatgaag ccggtggccc cggtcacgaa
      301 atactgcatt cggtgtctcc tcaggtggaa cgttcgctcg cggctgattg tgggacaagg
      361 ccgagtgcgg gcgcacgcgt gtttgtccga tgcaacgccg tcccccgcga tccccttgac
      421 ggggatagag cgaccgccct acgcaattag gtttcgccct ccacgcgcgg cgcctatatt
      481 tcagcctcga ccacgagtgg tggtcagccg cccgaacccc attcaagcaa caaaggatca
      541 cgacatggtc aagaagctct ccaagatcgc cgccaagaag gccgcctctc ccgccggcct
      601 gctcgacagc cagctcgcca gctccgtcaa ggaatcggcc cagcagatct ggctggccgg
      661 catgggcgcg ttcgccaagg cccaggccga aggcggcaag gtgttcgagg cgctggtcaa
      721 ggaaggcgtg agcctgcagc gcaagacgca ggccgtcgcg gaagagaagc tgggtgacgt
      781 caccggcaag atgagctcga tggcctcgga agtcaccagc aaggccggcg cgtcgtggga
      841 caagctcgag aacatcttcg aggaacgcac cgccaaggcg ctgggcaagc tgggcgtgcc
      901 caccgccaag gacgtggccg aactgacgcg ccgcgtcgag gaactgagcg ccgcggtggc
      961 caggctgtcg aagtcgggca aggccccggc cgctggcaag accgccgccg ccaaggcccc
     1021 ggcgaagaag gccgctgcca aggcgccggc caggaaggcc gccgccaggc gcgcgccggc
     1081 gaagaaggcc gctccggcct ccgccgccac ggccgcctga tcgttcggac ccgctcgcag
     1141 gccgcgtccc tcccgcggcc ccgcgccccc gaaggcgcca gcctcatcgc tggcgccttc
     1201 gtcatttgcg gactgaggta gagagctgcc tccagtgaac gggtccggga tctgccgatc
     1261 caccatcgac actcccgcga tgcccgacac catgagcaag acctccgatc ccgcagcgcg
     1321 cccacgcgcg cgagccccct cgcgcgcgcc ggcagccgca cccgcgcgca cgcgccgcca
     1381 cggcgcgcgc gccgtgggcc tcgcgctggc cggcggcggc ccgctgggcg cgatctggga
     1441 gatcggcgcg ctgtgcgcgc tggaggaggc gctggtgggc gtggacttca cgcgcatgga
     1501 cggctacgtc ggcatctcgg ccggcgcgtt catcgccgcg ggcctggcca atggcatgac
     1561 gccgcgccag atgtgcaccg ccttcatcga gaacgagggc ggcgacgacg acctggtcca
     1621 tccgtcgatc ttcgtgcacc ccgcgtggcg cgagttcgcc ggccgcctga agcgcctgcc
     1681 atcgctggtg gcgcaatcgg cgctgcgctt cgcgctcggg caggcctcgg gggtcgaggt
     1741 catcgagcgc ctgggccgcg tgctgcccac gggcgtgttc agcaacgcgc cgatcgaggc
     1801 gcacctgcgc caggtgttct ccgcgcccgg acgcaccgac gacttccgcg cgctgccgcg
     1861 ccgcctggtg ctggtggcca ccgacctgga caccggcgcc gtcgcgccgt tcggcctgcc
     1921 cggctgggac gacgtgccca tctcgcgcgc cgtcaccgcc agcgccgcgc tgcccggcct
     1981 gtacccgccg gtgcgcatcg gcgagcgcca ctacgtcgac ggcgcgctga agaagacgct
     2041 gcatgcgcgc gcgctgctcg acgaggacat cggcctcgtg ctgtgcctga acccgctggt
     2101 gccgttcgac gccacgtacg cgcccacgca caccgtgcgc ctgggcaccg accccatacc
     2161 gcgcctcgtc gagggcggcc tgccgctggt ggccagccag agcttccgct cgctgatcca
     2221 ctcgcggctg gagctgggcc tgaagggcta cggcctgagc cacccgcacg tggacatcgc
     2281 gctgttcgag cccgaccacc gcgaccccga gatgttcggc gccaacacct tcagctactc
     2341 ggcgcgccgc cagctggccg agcacgcgta ccagtcgacg cgcaagacgc tgcgcacgcg
     2401 gcgccgcgcg ctgcacgcga tgttcgagcc gcacggcatc ggccttgccg acggcgtgct
     2461 ggacgacccg acgcggcgcc tggtcaccta cacgccgcgc ctcgggccgg cctggcacga
     2521 cgtcgcgccc ggcaggctgg gcggcgcgct gcggcgcctg ggcggcgtgc tggacgagct
     2581 ggagtcgtcg ctcaagcccg cgtagtccgt cgcgtgccgc gcgccgccgt ccagcgcgcc
     2641 agcaccaccg gccggctcgc cgtgcgcacg acctggccga gctcggcgcg cgcgggctcg
     2701 gccaggtcga cgagcgacag caggaacacg cccgcgccgg tcgccaggta cagcgcgccg
     2761 tcgatgcgca tgaggcgcgg cgcgtcgaac accttggtcg acaccggcac gccgcgcacc
     2821 cggtggccgg cgcccagcac ctgcgacacc agccagggca cgcgcgcgat ccgcgccgcg
     2881 atgtcgttgc cgcgcgggcc tcgcaccagc gcgaacgccc cgctgatgaa cgatgcgacg
     2941 cgcggcgcgc cggcctgcgg cccggcggtc gcctcggcgt gcgcggcgct cgcgtcgacc
     3001 aggcggccga acgcacccca gcccagcagc gcgccgagcc gcttggcgac gatcgcgtcg
     3061 cgcagctgtc gcaggccggc ccaggcgtcg agctgctcga tgcggacctg cagccacgtg
     3121 gtgtcgacgg gctgcgcgga atccttcagc agctgatgca ggtccgcgat ctcgcgcagc
     3181 gtgaccggcg tggcgcgcgc gccgaacgcg gcgacgcggt gcagcacctg gcgcgtcggc
     3241 gcgagcgcct tgcatgccag gccgttcggc cgcaccgcgt cggcggcgag atcggcgaag
     3301 gcgacgtggc gcgtcgcgtc gatcggcgcg ccgcccacct gcacgcgtac gaccagcccc
     3361 gaacccagcg acggctcgta gtcgaaggcg gccaggccgt ggtgcatcgg cccgcgcggc
     3421 ggcacgtgcc actgcccggc atcgcccagc cggtactcga gccggcccag cgcctcgtgc
     3481 agcggaccga tggccgcgaa gtcgggcagc aggacgtcga tggcggcggc cgacgatcgc
     3541 gccgcctcgg aggccgccag caggctgccc ggcggcagcg cgcgcaggac gtcggcgacg
     3601 ccggcgcgac ggcgcacgat ctgttcgtgc tcgttggtct cggtcggcga cagcaggctc
     3661 tgcgcggccc agcgcgcata gagctcgggc aggcagcctt cctggcgcgc gacgaccagc
     3721 agctcccggc gcgccacgcg accggacgtc agcagcatct cggccgcggc gtcgccgtgc
     3781 ggcagggtga ggagctcgaa cagcccacgg tagagattgc tggtgctcat ggcgtcgttt
     3841 cctgtcccgc ggaagcggat cggcggaacc ggccgggaca tcgcagcgat tccgcgcgag
     3901 gcggcgggga tcgctcgccg actcgcgggc cgcatgctag ccagcgccgc gcgcgaaatc
     3961 gtcacgcgac gccgtatcag cttcgccggg ttcgcaacag ttgtgtccag cggccggcga
     4021 tcatgcgacg cgacgtgtcc gccgccccgg cgcggtcgcc cgcagcgcgg ccggcaggtc
     4081 ggcgggttgc atgtgcagcg aggcggtgtc gcgcaagtgc gccagcagcg cctccaccac
     4141 cacgcggacg cgcggggcca ggaactggcg gtgcgggtag acgagcacga actcgcgcgt
     4201 gccggcatcg tgggtgtcgg ccagcacggc cttgagccgg ccggcctgca ggaagggcaa
     4261 ggcctggtac agcccgcact gcgcgatgcc gccgccggcc agcgcgagct cgacgacggc
     4321 gtcgctgtcg gtgcccacca gcgcggcgtg cggggtgacg gcgacgcgcc gtccgttgac
     4381 gcggaacgtc cagggcgcgg cggtgccgtc ggcaaagcgc agctggatgc agcggtgccg
     4441 atcgaactcc gcgtgcgact gcgggacgcc ggcatgcgcg aggtagtccg ggctggccac
     4501 cagcaccagc ggcagcttgc acacgcggcg cgcgacgaag ccggcgtcgg gcaccgagcc
     4561 gccgcggatc gcgatgtcga tgccattggc cacgaggtcg accggcgcgt tgtcgagctc
     4621 gacctccagc accagcttcg ggaagcgcgc ggacagcgcc ggcagcgcgg gcagcaccca
     4681 catgcggccg aacgccacgc ccgtcgacag gcgcacgacg ccggcgggct cgcgcgccgc
     4741 ctgcgagacc tcggtgaccg cgtcgtccag cagccgcagc gcgtcgcgcg ccttggcgat
     4801 gaagcgctcg ccctcgcccg tgacgcgcgc ggtgcgcgtg ctgcgctgga ccagccgcac
     4861 gcccagctgc tcttccagcc gcgccaggct cttgctgacc gccgcgggcg tgacgcccag
     4921 ccggcgcgcg gcgcccgcga agctgccagc ggcggcgatc tcgatcagcg cgagcagtcc
     4981 ctgcagttgc ttcatgtgca cgattctcaa ccgaaagtca ctgctgcatc aacctgtcgt
     5041 gactttccag acatgccaga catgcctaga gttcgctcca tgcccgggac gttccgggcg
     5101 ccaaccagga gttccaccat gaagatcggc atcatcggat cgggcgtcgt cgcgcagacg
     5161 ctcgccgcag ggttcatcaa gcacggccac caagtgacgc tgggcacgcg cgacgtgtcc
     5221 aagctcgcgg acttcgccgt gaagacgccc ggcgcgcgtg tggccggcag cgccgaggcc
     5281 gcggccttcg gcgaggtcgt ggtgctggcg gtcaagggca gcgccgcgct gcaggccgcg
     5341 cgcgacgcgg gcgccgcgct cgacggcaag cccgtgctcg acgcgaccaa cccgatcgcc
     5401 gacgccgcgc ccgtcgatgg cgtgctgccc tacttcaccg gcaagaacga atcgctgatg
     5461 gagtggctgc aggaggcgca tccgcgcgcc cgcttcgtca aggcattcaa ctcggtcggc
     5521 gcggcccgca tggtcgaccc cgacttcggc ggcacgcgcc cgaccatgtt catcgccggc
     5581 aacgacgccg gcgccaagga cgtggcgacc acgctgctcg cgcagctggg ctgggacacc
     5641 gccgacatgg gcacggccgt cgccgcccgc gccatcgagc cgctggccat gctgtggtgc
     5701 atcccgggct tccgcgaagg gtcgtggatg catgcgttca agctgctgcg ctgaaagccg
     5761 ccgcggggcg ccgcgcctgt acattccggt gatggcacct cgcacctgga tcgtcgactc
     5821 ggtcggagcg gcaacgttcg agcggctggc gtcgggactc gcgggctcgg agctccagtc
     5881 gctgctgctg gacgtgatgc aggcgcgcgc cgcatcgcgc agcgcggccg acctgctggc
     5941 gcagcacggc cgcgacgcgt tctgcgtgcc ggcacaggtc gacgcccgcg cgatgctggc
     6001 catcgacgcc cgcctgttcg aagccgccga gggcttcgat gcgatcgagc tgtcgcccgt
     6061 cgcgccgctg ggcgcgtgct ccgtggtcgg cccgaccgac cagaaccgcg tgctctccgc
     6121 gctgcgcgcc accgaggtcg tctcggatcc gacgaacgtg ctcgcgctcg aatgcgcgca
     6181 gcggctgcgc gcgcgccctg gcgtggcgca ccacctggcc acgagccacc gcgtgatgcg
     6241 cgcgcagccg gttcccaagc tcccgggcta cgcgcagcac ttccggctgt tcgcgctcgc
     6301 gagcggcggt gtcgagcgcg aggaccacgc cttcaccgtc gacatcctgg ccctgcacgt
     6361 gcgcacgatg ctggcgggtc tcgaccgcct cgtgcagcag ggcttcgcgc tcgaacgccg
     6421 gcgcatcgag atcctcgcga cgcccgcgcg ccagccgctc ggcgagcgac tcgccaaggc
     6481 gctggatgcc gaactcggcg cgctggacca cccatactac tcgggcgg
//
LOCUS       DATCRO010000066         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_878280, whole genome shotgun sequence.
ACCESSION   DATCRO010000066 DATCRO010000000
VERSION     DATCRO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878280"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VIN75_01825"
     CDS             complement(<1..1313)
                     /locus_tag="VIN75_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY8972927.1"
                     /translation="MSTITVYVPRDSAALAVGADSVARRIAKEAAARGLMLEIVRNGS
                     RGMFWLEPLVEVNTPAGRIAYGPVTPDDVAGLFDAGFLTGGRHPLALGPTEEIPYLKK
                     QERLTFSRMGITDPVSLTDYQAYEGFAGLRRALALEPGEIVQQVLDSGLRGRGGAAFP
                     AGIKWKTVLGTAAAQKYIVCNADEGDSGTFSDRMTMEGDPFMLIEGMTIAGLATGATK
                     GYIYVRSEYPHAIATLNEAIRVAEAAGFLGDNILRSGKRFSLEVRKGAGSYVYGEETA
                     LLESLEGKRGIVRAKPPLPAIEGLFGRPTLINNVITLASVPLILARGAAFYRDYGVGR
                     SRGTLPMQLAGNIRYGGLVEKAFGVTLRELLYDYGGGSASGRPIKAVQVGGPLGAYLP
                     ESQWDIPLDYEAYAAVGAVVGHGGIVVHDDTADMAKLARYAMEFCA"
     gene            complement(1310..1810)
                     /locus_tag="VIN75_01830"
     CDS             complement(1310..1810)
                     /locus_tag="VIN75_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY8972928.1"
                     /translation="MRQEQVQQAQQGDETQELATVRRILQAHENEPGALLPILHDVQD
                     ALGHIPANAVPVIAEAQNLSRAEVHGVVTYYHHFRSAPAGRHVIQICRAEACQSMGAD
                     ALLAHAELRLGCSAHGTTKDGAFTLEPAFCLGLCASSPAMTLDDQPHARVTPRTFDAL
                     LAQVAP"
     gene            complement(2073..>2373)
                     /locus_tag="VIN75_01835"
     CDS             complement(2073..>2373)
                     /locus_tag="VIN75_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY8972929.1"
                     /translation="LHADPGFPLTIGADVTVGPHVMLHGCTVGDGAAIGENTVALNGS
                     VVGRGCIVEPGSLLTEGKVFPDRSRIAGRPAKVAGEVSDEEAQAQLRRAREAAAR"
BASE COUNT          338 a          817 c          848 g          370 t
ORIGIN      
        1 gcgcagaact ccatcgcgta gcgggcgagc ttggccatgt ctgcggtgtc gtcgtgcacg
       61 acgatcccgc cgtggccgac gacggcgccg accgccgcgt aagcctcgta gtcgagcgga
      121 atgtcccatt gcgattcggg caggtaggcg cccaggggcc caccgacctg cacggccttg
      181 atgggccggc ccgaggccga gcctccgccg tagtcgtaga gcagctcgcg cagcgtgacg
      241 ccgaaggcct tctcgaccag gccgccgtac ctgatgttgc cggccagctg catcggcagc
      301 gtgccgcgcg agcggcccac gccgtagtcg cgatagaagg ccgcgccgcg cgcgaggatc
      361 agcggcacgc tcgccagcgt gatgacgttg ttgatgagcg tcggccgccc gaacagcccc
      421 tcgatggccg gcagcggcgg cttggcgcgc acgatgccgc gcttgccttc caggctctcg
      481 agcagcgccg tctcttctcc gtacacgtac gagcccgcgc cctttcgcac ttcgagcgag
      541 aagcgcttgc ccgaacgcag gatgttgtcg ccgaggaagc ccgcagcctc ggcgacgcgg
      601 atggcctcgt tcagcgtcgc gatcgcatgc gggtactcgg aacgcacgta gatgtagccc
      661 ttcgtcgcgc cggtggccag gcccgcgatg gtcatgccct cgatcagcat gaaggggtcg
      721 ccctccatcg tcatgcggtc ggagaaggtg cccgagtcgc cctcgtcggc attgcagacg
      781 atgtacttct gcgccgccgc cgtgcccagc accgtcttcc acttgatgcc cgccggaaag
      841 gccgcgccgc cgcgcccgcg caggccggag tcgagcacct gctgcacgat ctcgccgggc
      901 tcgagcgcca gcgcacggcg caggccggcg aagccttcgt aggcctggta gtcggtgagc
      961 gagaccgggt cggtgatgcc catgcgcgag aaggtcaggc gctcctgctt cttcaggtac
     1021 gggatctcct cggtcggacc gagcgcgagc ggatgcctgc cgccggtgag gaagccggcg
     1081 tcgaacaggc ccgccacgtc gtcgggcgtc accggcccgt aggcgatgcg gccggcgggc
     1141 gtgttcacct ccaccagcgg ctcgagccag aacatgccgc gcgaaccgtt gcgcacgatc
     1201 tcgagcatca gcccgcgcgc ggccgcttcc ttggcgatgc gccgggccac gctgtcggct
     1261 ccgacggcga gcgcggccga gtcgcgcgga acgtagacgg tgatggtgct catggggcga
     1321 cctgcgcgag gagcgcgtcg aacgtgcggg gtgtcacgcg cgcgtgcggc tggtcgtcca
     1381 gcgtcatcgc aggcgaggag gcgcacaggc cgaggcagaa ggccggctcc agcgtgaagg
     1441 cgccgtcctt cgtggtgccg tgcgcgctgc agcccagccg cagttcggca tgggccagca
     1501 gcgcatcggc gcccatcgac tggcacgcct cggcgcggca gatctggatc acgtggcggc
     1561 cggcgggcgc gctgcggaag tggtggtagt aggtgaccac gccgtgcacc tcggcgcgcg
     1621 agaggttctg cgcctcggcg atgaccggca ccgcgttcgc gggaatgtgg ccgagcgcgt
     1681 cctgcacgtc gtgcagtatc ggcagcagcg cgccgggttc gttctcgtgc gcctgcagga
     1741 tgcgccgcac cgtggcgagc tcttgcgtct cgtccccctg ctgcgcctgc tgaacctgtt
     1801 cttgtctcat ggtccgtccg atctgctccg ccgctgcatg aagcctcggg ggcctgcagg
     1861 cggagctggt ttcatcgtgg ttccgtcacg ggcccggcgt ctcgaattcg acgcgaatcc
     1921 cgtggtggtc cgcttgggga ccaatgtcga tgaatcgggc gtcgggggcc ttgacacaag
     1981 tcaatttccg ccgaaacagg cacggtgcca atgcgcacaa accctgcggg cgtgcagggt
     2041 tcgcaaggac cgcaagaact gtcggaaaga ccctagcggg cggcggcttc gcgcgccctg
     2101 cgcagctgcg cttgcgcctc ttcgtcgctc acttcgccgg cgaccttcgc ggggcggccc
     2161 gcgatgcgcg aacggtcggg aaagaccttg ccttcggtga gcagcgaacc gggctcgacg
     2221 atgcagccgc ggcccaccac cgagccgttg agcgcgacgg tgttctcgcc gatggcggcg
     2281 ccgtcgccca cggtgcagcc gtgcagcatc acgtgcgggc ccaccgtcac gtcggcgccg
     2341 atggtgagcg ggaagccggg gtcggcatgc agc
//
LOCUS       DATCRO010000067        12792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_270736, whole genome shotgun sequence.
ACCESSION   DATCRO010000067 DATCRO010000000
VERSION     DATCRO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270736"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1615
                     /locus_tag="VIN75_01840"
     CDS             176..1615
                     /locus_tag="VIN75_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8972930.1"
                     /translation="MDLARTQRYLPWLVATALFMEQLDSTIVNTGIPSMAASLGVTPL
                     SLKAVAASYILALAVSIPVSGWLADRFGSRRIFLFALGMFTVSSVLCGLAPNASLLVA
                     SRVPQGISAAMMMPVGRSAVVRTFPKSELLRVMNFIIIPALIGPLLGPTVGGLIVHWL
                     NWRVMFFVNVPVGLVGFWLGRRVMPDYRTEDPPPLDVVGLVLFGSGVALLSWLLEVFG
                     EHSVPAFQVTLLFLLSAALLGAYAWHATREPFPLLRLGVFKVRTFRISVLGGFVTRLG
                     IGGLPFLLPLLYQLGLGLPAWESGLLMMPAAASAMFMKVLSSSLLRRFGYRRVLTANT
                     VLVACCIACYSLVGPGTWLGVIVALGATMGLFNSLQFSSLNTMAYADIDSGDASMAST
                     IASTLQQMSLSFGLAMATLVTSFFLADLPQSDHVAVTRALHHGYLMMASLTLLSSLAF
                     WGLRRNDGESVSKGAPAREAAESRHSELA"
     gene            complement(2313..2630)
                     /locus_tag="VIN75_01845"
     CDS             complement(2313..2630)
                     /locus_tag="VIN75_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase with chaperone activity"
                     /protein_id="HEY8972931.1"
                     /translation="MSDENQIEIPPSFIALFVEPGRIKPNATRAEITRRYEFCEALAT
                     MLVERAQTLQWQLGITEADVLERLHAGLRGPDAPVPPNEGEWIISRLAELLEWERPKW
                     KAP"
     gene            2740..3735
                     /locus_tag="VIN75_01850"
     CDS             2740..3735
                     /locus_tag="VIN75_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8972932.1"
                     /translation="MRDLIPLLIAHGALIVFVITLAARVGAPVPAAPLLVVAGGVAMA
                     GQISLLVCVGVSVVANVLGDAVWYQAGRWRGHRVMKLLCRISLSPDTCVRQSEGILSR
                     WGGSSLIAAKFLPGISVVAAPMAGALGMSWRRFISYDIGASLIWTLVFLAIGVIFAGQ
                     VVMVLDVMTEFGTIAAVAIVIALAALITGRWLRRRWMLSDRYAPRIAVHELRELIRRG
                     EEPIVIDVRSAVATLHDNRRVPGAILATLADLPATAPGLPRDREIVLYCNCPNEVSAL
                     RGVRILADHGHHRARALRGGLDAWIEIEAVSPLGQQPGGLDQGALSPMAVPEGSR"
     gene            complement(3758..4192)
                     /gene="trxC"
                     /locus_tag="VIN75_01855"
     CDS             complement(3758..4192)
                     /gene="trxC"
                     /locus_tag="VIN75_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY8972933.1"
                     /translation="MLLVCPHCAATNRVPEERLGDAPACAKCGTALADSKPFDLAEAV
                     FPKYIRATEAPVLVDFWAAWCGPCRQMAPHFAAAAQQLPDVRFAKVDSDAAQQASAAY
                     GIRSIPTLILFKGGKEVARRSGASSAGDIAAWTRAQLARTGP"
     gene            complement(4242..4991)
                     /locus_tag="VIN75_01860"
     CDS             complement(4242..4991)
                     /locus_tag="VIN75_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8972934.1"
                     /translation="MITCLIVDDDPALRELVGDHLTRYGLTARLADSAAAMRRELARG
                     GIDIVLLDLMLPDGDGIELCRDLRMHSKLPVIMLTAQGDPISRVLGLEMGADDYVAKP
                     FEPRELLARIHALIRRSSGTLAPGAADPAGEAAAPQFAPPGLPASGGFGGWQIDWRNR
                     MLISPEGTGLSLSNVEYRLLAAFAEHPGEVLSRGQLVEMTRSAASDNNERSIDLAVSR
                     LRAKLRESLENPQFIRTVRGRGYQFTAELAR"
     gene            complement(5154..5855)
                     /locus_tag="VIN75_01865"
     CDS             complement(5154..5855)
                     /locus_tag="VIN75_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY8972935.1"
                     /translation="MTALRRVLALSFLAIAGAMTGCASNTPLVQGPTSVRPVPPPTYA
                     ERTVTGGIYRANLGQVSLFSDQRKPQAIGDTIKIDISESLDASSVVNSVNSRENAVSS
                     KGPGMNSNSLGTLLKGLANMDATASGSDSFTGKGNSQNTAKFNARLSAFVVNVLSNGN
                     LVVAGERSMSFNGTLNTLRFSGVVNPLDIKAGGIVASGDVADAKTELVGSGGTADTAS
                     RGWLQKLLTDGLSVW"
     gene            complement(5889..6428)
                     /locus_tag="VIN75_01870"
     CDS             complement(5889..6428)
                     /locus_tag="VIN75_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEY8972936.1"
                     /translation="MRPLTFLALAAAVVVLPGCSIVSPAPVLELVKATGAVAVSAISH
                     GPSSAKNTVYHEHKALDSVCVEYNPLTPDPDIAPALQAELRTHEIDSRIYEEGGAPST
                     CKFRVTYTADIEWGIPPFGSGYKSYMRDATLTLRDENGTVLSSSAYALDGTFQMGKWS
                     TSRSKLAPVVTALVTGFDH"
     gene            complement(6458..7195)
                     /locus_tag="VIN75_01875"
     CDS             complement(6458..7195)
                     /locus_tag="VIN75_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972937.1"
                     /translation="MTSVTSSTSTISSPFATVREQIRPARPESADQGDTASLSGAWQG
                     VGDAIAEGASATVSLSEKAMHAVEDGASYIGNAVETGIADVGAAAMAAYAAVRNGVNN
                     TVQGVENAGEAVWNTVKAGAEDVAGMAGDVVHAAEAGVQAVGDVAVDAWHGVEHLASE
                     TLHGIEKGTGELEHLGSQAWQAVENGTHRMRDYAGQAGEGVRTAAGYVGDGIGDVADA
                     VGNVAGRMASYATVGAAAVERAFTAVA"
     gene            7447..10041
                     /locus_tag="VIN75_01880"
     CDS             7447..10041
                     /locus_tag="VIN75_01880"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8972938.1"
                     /translation="MDAGDAVPDSRLSAAPVDSTGSGVTFLLNQPADRRQRWRAGLVL
                     LVSVLVFGALVPFVRVPLGFLPAFIPIYESALLINDMVTAVLLFGQYRILRSRALMVL
                     ACAYLFTALMTVVHALSYPGVFAEGGLLGAGRQTTASLYMFWHAGFPLFVLAYTVLKQ
                     RERQDATAAAANEQAWFAPARPHDRVALALPVATVALAALLTLYATVGQAWLPTFLYP
                     GHFGLALTFSAAAIWALSLAGVAALWRHRPHSVLDLWLSVSLVAWSFDVALSSLLNAQ
                     RFDFGFYAGRVYGLIAGSAVLIELLLENGALYARLVRVHHNDRRQAAALRIARDEARS
                     ADQAKGLFLANMSHEIRTPMNAVIGLTAMVLQTRLDETQRDYLSKVQTSSKALLTLLN
                     DILDYSKIEAGAVSLEAEEFSPEETIENVGNLFSAKVEEARLDLLFEIDRDMPPRLVG
                     DALRLAQVLNNLVGNAIKFTSVGEVVIGACVVSRSDEQVELAFSVRDTGIGVTPEQAE
                     RLFNVFQQAERSTARKYGGTGLGLAICRRLVELMGGRIEVTPAPGGGSIFGFTCKFAI
                     ARGASERIDLHRIRGMRTLVVDGQPTERLILQQMLQSWRFQVGTASFPDDALHKLRRA
                     DPSAPHELMLLDWKTGDLDLLLKARRAVEEHRSQPGAAPLSVIAMTSLAARERVAESL
                     KGLPGVDIVTKPVTPSRLFDTVVRLQHGEAAPTPRTGERQFDLEESMRPIRGARVLLA
                     EDNPVNQQVATAFLQAGGLVVTLAENGVEAVEWVRKAHFDVVLMDMQMPDMDGTQATR
                     LIRKLPQGMGLPIIAMTAAAMDSDRQECLAAGMNAHVAKPIDAEKLVRTLLEWVSPRG
                     IARGRPAS"
     gene            complement(10048..12480)
                     /locus_tag="VIN75_01885"
     CDS             complement(10048..12480)
                     /locus_tag="VIN75_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HEY8972939.1"
                     /translation="MATVRPLVRFVVVGVLLILVGLALIVGGVVLATAGGSPFYLLAG
                     IGFGAAGVLLWRGQAEALIVFAVLLAAMLAWSLWEVGLDWWPLAARLDLAFVFGALLV
                     LPWISRHLRGPRSGRIVLALVVGVSAVVAIGSGLRDTHEIAGQLAEAGQGTAVAASGV
                     PDGEWHAYGRTAAGERWSPLAQITPGNVDRLKVAWQFRTGDVRGRPGDPTETTDEVTP
                     LKIGDRLYLCTPHQSVIALDATTGRLAWRYDPKMETGLALQHQTCRGLSYEPPPEAAA
                     APAPAASVALRTSGRHLDIAANDGKARADCGAKLFLATADGRLIALDPETGAVCSRFG
                     AGAGQIDLGANMPNLNPGSYYSTSPVIVTKKLVIVGGTVLDNFSTKEESGVIRAFDID
                     SGALVWNWDSGNPDVTTPIAADATYTANSPNSWSIGSVDEALGLVYLPMGNQPPDQWG
                     GRRSPAVEKYSSSVVALDLATGRVRWVFQTVHHDLWDYDVPSQPSLVDLTVNGTTVPA
                     LVQPTKQGELFVLDRRTGQPLLPVTEVPAPQGAAAGDHSAPTQPKSALSYEPPKLAGK
                     DMWGGTIIDQMMCRIALHKLRYEGRFTPPSTQGALIYPGNFGTFNWGGVAVDPQRQVA
                     FTTPTYLAFVSTLVPRPNPTVELVQNGKPPQGALPALNENFGAPFAVKLHPFTSPLGI
                     PCQAPPWGYVAGADLRTGRIAWEHRNGTVRDLSPIPLPLRMGVPNLGGPVMTGGGVAF
                     LSGSMDDYVRGYDVRTGRQLWEDRLPAGGQATPMTYLGADGRQYLLVIAGGHGSTGTK
                     PGDFVIAYALPR"
     gene            complement(12570..>12792)
                     /locus_tag="VIN75_01890"
     CDS             complement(12570..>12792)
                     /locus_tag="VIN75_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972940.1"
                     /translation="APLLGVLLTLAFTIPPFQLMVYLGFFAGFLLYIGVSDILPEAHS
                     RAGPGVAVKLIALTCCGAAFVWLVVRQSA"
BASE COUNT         1719 a         4489 c         4583 g         2001 t
ORIGIN      
        1 gcgcgcttgg cggcgggggt ggacatgtcc agggtgtcgg cgacttcgaa gctcatggcg
       61 tcggatgaca gggcaaatgg cggtacggga ttgtcgtcga gccgcccgcc cgcgccggcc
      121 gcaggggaaa ccatgcgcgc ccgtcgaaac gcgcggcgca cgagaatgga ccgcgatgga
      181 tctcgcccgc acccagcgct acctgccctg gctcgtcgcg accgcgctgt tcatggagca
      241 gctggactcg acgatcgtca acaccggcat cccgtcgatg gcggccagcc tgggcgtcac
      301 gcccctgagc ctgaaggcgg tggccgccag ctacatcctg gcgctggccg tctcgatccc
      361 ggtcagcggc tggctggccg accgcttcgg cagccggcgc atcttcctgt tcgcgctggg
      421 catgttcacc gtctcgtcgg tgctgtgcgg cctggcgccc aacgccagcc tgctggtggc
      481 ctcgcgcgtg ccgcagggca tctccgccgc gatgatgatg ccggtgggcc gaagcgccgt
      541 ggtgcgcacc ttcccgaagt ccgagctcct gcgggtgatg aacttcatca tcatcccggc
      601 cctgatcggc ccgctgctgg gcccgaccgt cggcggcctg atcgtgcact ggctcaactg
      661 gcgggtcatg ttcttcgtca acgtgcctgt cggcctggtc ggcttctggc tgggccggcg
      721 ggtcatgccc gactaccgca ccgaggatcc gccgccgctg gacgtcgtcg gcctggtgct
      781 gttcggctcg ggcgtggcgc tgctgtcgtg gctgctggag gtgttcggcg agcactcggt
      841 gccggcgttc caggtcacgc tgctgttcct gctgtcggcg gcgctgctgg gggcctacgc
      901 ctggcacgcg acgcgcgaac ccttcccgct gctgcgcctg ggggtgttca aggtgcgcac
      961 cttccgcatc tcggtgctgg gcggcttcgt cacgcgcctg ggcatcggcg gcctgccctt
     1021 cctgctgccg ctgctgtacc agctgggcct ggggctgccg gcctgggagt cgggcctgct
     1081 gatgatgccg gcggcggcgt cggcgatgtt catgaaggtg ctgtcgtcgt cgctgctgcg
     1141 gcggttcggc taccgccggg tgctcaccgc caacaccgtg ctggtcgcct gctgcatcgc
     1201 ctgctactcg ctggtcgggc cggggacgtg gctcggcgtg atcgtcgcgc tgggcgccac
     1261 gatggggttg ttcaactcgc tgcagttctc cagcctcaac acgatggcct acgccgacat
     1321 cgactcgggc gacgcctcca tggccagcac catcgccagc acgctgcagc agatgtcgct
     1381 gagcttcggg ctggcgatgg ccacgctggt cacgtcgttc ttcctggccg acctgcccca
     1441 gtccgaccac gtcgccgtca cgcgtgccct gcaccacggc tacctgatga tggcctcgct
     1501 gaccctgctg tcctcgctgg ccttctgggg cctgcgccgc aacgacggcg agagcgtcag
     1561 caagggggcc cccgcccgcg aggcggccga atcgcggcat tcggaactgg cctgaaaccg
     1621 ggcgcttctc cgccgatcgg cggtgcgccg catccggaga atacgaagtg tccgaggtac
     1681 gcaaccggcc gcaaattggg cggaaagatg ggaacccgtc acccagtacc cgcaggaaac
     1741 cgtcgtctgg accagacgtt cggtgatgtt gcccgcagtc cagcaatggt tgcgggcatc
     1801 gtcttttggg cggcggattt tctcagggcg ccttccactt cggccgctcc gactccagga
     1861 gttcggccag tcgcgtgact gggtgccgac tgggtgccac gactgggtgc caggtctggc
     1921 ctggcaagtc gctcgcgtgt tgccgggcaa gacctggcac cgccgagaaa tcttgggggt
     1981 ggatcttgtc agggcgcctt ccacttcggc cgctcccact ccagcacttc ggccagccgc
     2041 gtgactgggt gccaggtctg gcctgaccgg gtgccaggtc tggcctggca agtcgatcgc
     2101 gtgttgccgg gcaagacctg gcaccgccgg gactctttgg gcggcggatc ttctcagggc
     2161 gccttccact tcggccgctc ccgctgcagc agttcggcca gccgcgtgac agggtgccag
     2221 gtctggcctg gcaagtcgat cgcgtgttgc cgggcaagac ctggcaccgc cggggggtgg
     2281 caccgtcggg actctttgga cggtggatcc tctcagggcg ccttccactt cggccgctcc
     2341 cactccagca gttcggccag ccgcgagatg atccactcgc cctcgttcgg cggcaccggg
     2401 gcgtccgggc cgcgcaggcc ggcgtgcagg cgttccagga cgtcggcttc cgtgatgccc
     2461 agctgccact gcagggtctg cgcgcgttcg accagcatcg tggcgagcgc ctcgcagaac
     2521 tcgtagcgcc gcgtgatctc ggctcgcgtc gcgttcggct tgatgcgtcc gggttcgacg
     2581 aacagcgcga tgaacgacgg cgggatctcg atctggttct cgtctgacat ccccggattg
     2641 tcgccgccgc gcgctcccgg ccgacagccc tgcggcgaac gtgcctcggc cggccctgta
     2701 tccttccggc atctcgtccg ctcgcctgcc cttcccgcca tgcgcgacct catccccctg
     2761 ctcatcgccc acggtgcgct gatcgtcttc gtgatcacgc tggcggcgcg cgtgggcgcg
     2821 ccggtgcccg cggcgccgct gctggtggtc gccggcggcg tggcgatggc gggccagatc
     2881 tcgctgctcg tgtgcgttgg cgtctcggtg gtcgccaacg tgctgggcga cgccgtctgg
     2941 taccaggccg ggcgctggcg cggtcatcgg gtgatgaagc ttctgtgccg catctcgctg
     3001 tcgcccgaca cctgcgtgcg ccagagcgaa ggcatcctgt cgcgctgggg cggcagctcg
     3061 ctgatcgccg ccaagttcct gccgggcatc tcggtcgtgg cggcgccgat ggccggcgcg
     3121 ctgggcatgt cctggcgccg cttcatctcg tacgacatcg gcgcctcgct gatctggacg
     3181 ctggtgttcc tggcgatcgg cgtgatcttc gccggccagg tcgtgatggt gctggacgtc
     3241 atgaccgagt tcggcaccat cgccgcggtc gccatcgtca tcgcgctcgc cgcgctgatc
     3301 accggccgct ggctgcgccg gcgctggatg ctcagcgatc gctacgcccc gcgcatcgcc
     3361 gtgcacgagc tgcgcgagct gatccgccgc ggcgaggagc cgatcgtgat cgacgtgcgc
     3421 tcggcggtcg ccacgctgca tgacaaccgc cgcgtgccgg gcgcgatcct cgccacgctg
     3481 gccgacctgc ccgccaccgc accgggcctg ccgcgcgacc gcgagatcgt cctgtactgc
     3541 aactgcccca acgaggtgtc cgcgctgcgc ggcgtgcgca tcctcgccga ccacgggcac
     3601 caccgggcac gcgcgctgcg cggcgggctc gacgcctgga tcgagatcga ggccgtctcg
     3661 ccgctgggcc agcagccggg cggcctcgat cagggcgcgc tctcgcccat ggccgtgccc
     3721 gaaggctccc gctgagcgcc tcgcgtgccc tggcgcgtca cgggcccgtg cgcgccagct
     3781 gcgcccgcgt ccacgcggcg atgtcgcccg cggacgaagc gcccgagcgg cgcgccactt
     3841 ccttgccgcc cttgaacagg atcagcgtcg ggatgctgcg gatcccgtag gccgcgctcg
     3901 cctgctgcgc cgcatcgctg tcgaccttgg cgaagcgtac gtcgggcagt tgctgcgcgg
     3961 ccgccgcgaa gtgcggcgcc atctgccggc atggcccgca ccaggcggcc cagaagtcga
     4021 ccagcaccgg cgcctccgtg gcgcggatgt acttcgggaa caccgcctcg gccaggtcga
     4081 acggcttcga atcggccagg gccgtgccgc acttggcgca ggcgggcgcg tcgcccaggc
     4141 gctcctcggg gacgcggttg gtggcggcgc agtgggggca gacgagcagc atggcggttc
     4201 ctcggttcgc gcggtcgcgt tccccgaggc gaatgctgtg cctaccgggc cagttcggcg
     4261 gtgaactggt agccgcgccc tctcacggtg cggatgaact gcggattctc aagggactcg
     4321 cgcagcttgg cgcgcagccg cgagaccgcc aggtcgatcg agcgctcgtt gttgtcgctg
     4381 gcggcgctgc gcgtcatctc gaccagctgg ccgcggctga gcacctcgcc cggatgctcg
     4441 gcgaaggccg ccagcagccg gtattcgacg ttggacagcg acaggcccgt gccctcgggg
     4501 ctgatcagca tgcggttgcg ccagtcgatc tgccagccgc cgaacccgcc gctcgcgggc
     4561 aggcccgggg gcgcgaactg cggcgcggcg gcctcgccgg cggggtcggc cgcgcccggg
     4621 gccagcgtgc cggagctgcg gcggatcagc gcgtggatgc gcgcgagcag ctcgcgcggc
     4681 tcgaacggct tggcgacgta gtcgtcggcg cccatctcca gccccagcac gcggctgatc
     4741 ggatcgccct gggcggtcag catgatcacg ggcagtttcg aatgcatgcg caggtcgcgg
     4801 cacagctcga tgccgtcgcc gtcgggcagc atcaggtcca gcagcacgat gtcgatgccg
     4861 ccgcgggcca gttcgcgccg catcgcggcg gcgctgtcgg ccaggcgcgc cgtcaggccg
     4921 tagcgcgtga ggtggtcgcc gacgagctct cgcagcgcgg ggtcgtcgtc gacgatgagg
     4981 caggtgatca tttcaggacg gacaggaacg ggaggtgacg cgagccagca tagcgtgcac
     5041 ggtcgtgcaa gtcatgtcgg ctgcgtgtcg gtccggacac aaacgagaac ggcttcgcca
     5101 acgaggcgaa gccgtggaga tcgtgccgga acgaggcccg ccgggccccg ccgtcaccag
     5161 accgacaggc cgtccgtgag cagcttctgc agccagccgc gcgacgcggt gtcggccgtg
     5221 ccgccgctgc ccaccagctc ggtcttggcg tcggccacgt cccccgaggc gacgatgccg
     5281 ccggccttga tgtccagcgg gttgaccacg cccgagaagc gcagcgtgtt gagcgtgccg
     5341 ttgaacgaca tgctgcgctc gccggccacc accaggttgc cgttggacag cacgttgacc
     5401 acgaaggccg acagccgcgc gttgaacttg gcggtgttct gcgagttgcc cttgccggtg
     5461 aacgagtcgc tgcccgaggc ggtggcgtcc atgttggcca ggcccttgag cagcgtgccc
     5521 aggctgttgg aattcatgcc cgggcccttg gacgagacgg cgttctcgcg gctgttgacg
     5581 ctgttgacga cgctgctggc gtccaggctt tccgagatgt cgatcttgat cgtgtcgccg
     5641 atggcctgcg gcttgcgctg gtcggagaac agcgagacct ggcccaggtt ggcgcggtag
     5701 atgccgccgg tgacggtgcg ctcggcgtag gtcggcggcg gcaccgggcg cacggaggtc
     5761 gggccctgca ccagcggggt gttggaggcg cagcccgtca tcgcgccggc gatggccagg
     5821 aaagacaggg cgaggacgcg gcgcaaggcg gtcacgggag atcctttgag catgtggtgc
     5881 gggggcgttc agtggtcgaa gcctgtgacc agcgcggtca cgaccggcgc cagcttgctg
     5941 cggctggtgg accacttgcc catctggaag gtgccgtcca gggcgtaggc gctgctggaa
     6001 agcacggtgc cgttctcgtc gcgcagcgtc agcgtggcgt cgcgcatgta ggacttgtag
     6061 ccgctgccga acggcgggat gccccactcg atgtcggcgg tgtaggtgac gcggaacttg
     6121 caggtggacg gcgccccgcc ctcctcgtag atgcggctgt cgatctcgtg cgtgcgcagc
     6181 tcggcctgca gcgccggggc gatgtccggg tcgggtgtca gcgggttgta ttcgacgcag
     6241 accgagtcga gcgccttgtg ctcgtgatac acggtgttct tggcgctgct ggggccgtgc
     6301 gagatggcgc tgacggcgac ggcgccggtg gccttgacca gttccagcac gggcgccggg
     6361 ctgacgatgg agcagcccgg cagcacgacg acggccgcgg ccagggcaag gaacgtgaga
     6421 gggcgcatgg ggatggaatc gtgtttcggt ggcgggctca ggcgacggcg gtgaaggcgc
     6481 gctcgacggc ggcggccccg accgtggcgt agctggccat gcggcctgcg acgttgccga
     6541 ccgcgtcggc gacatcgccg atgccgtcgc cgacgtagcc cgcggcggtc ctgacgccct
     6601 cgcccgcctg gccggcgtag tcgcgcatgc ggtgcgtgcc gttctcgacc gcctgccagg
     6661 cctggctgcc caggtgctcg agctcgccgg tgcctttctc gatgccgtgc agcgtctcgg
     6721 aggccaggtg ctcgacgccg tgccaggcgt cgacggcgac gtcgcccacg gcctggacgc
     6781 cggcttcagc ggcatgcacc acgtcgccgg ccatgcccgc gacgtcctcg gcgcccgcct
     6841 tgacggtatt ccatacggct tcgccggcgt tctcgacgcc ctgcaccgtg ttgttgacgc
     6901 cgttcctgac cgccgcgtag gcggccatcg cggcggcgcc gacgtcggcg atgcccgtct
     6961 cgacggcgtt gccgatgtag ctggccccgt cctcgacggc atgcatggcc ttctcgctca
     7021 gggagaccgt ggcgctcgcg ccctcggcga tcgcgtcccc gacgccctgc cacgcgccgg
     7081 acaggctggc ggtgtccccc tggtcggccg actcgggccg ggccggccgg atctgctccc
     7141 ggaccgtggc gaacggcgag ctgatcgtgc tggtggagga agtgacggac gtcatggacg
     7201 gactcctgga ttcggatgat cgaatcgtag agatcccgcg ccgggcgtaa tcggcgatca
     7261 agaccggaaa tgaagggcaa ttcagccccc gtcaggcaaa aagttcttta cccgaccgcc
     7321 gccggcgata gcgaaccccc gccggcctcg caggcattcc ggtccgggtt ggacgacgtg
     7381 caacacgcgc ctatcctgtc gcgagcgatg cccgacggca tggatcgccg ggccagggag
     7441 cgagccatgg acgctggaga cgcagtaccg gacagccgac tgtccgccgc ccccgtcgat
     7501 tcgaccggga gcggcgtcac gtttctcctg aaccagccgg ccgaccgccg gcagcgctgg
     7561 cgggcgggcc tcgtcctgct cgtctcggtg ctcgtgttcg gcgccctggt gccgttcgtg
     7621 cgggtgccgc tgggcttcct gccggccttc atcccgatct acgagtcggc gctgctgatc
     7681 aacgacatgg tcacggcggt gctgctgttc ggccagtacc gcatcctgcg ctcgcgggcg
     7741 ctgatggtgt tggcctgcgc ctacctgttc acggcgctga tgacggtggt ccacgcgctc
     7801 agctatcccg gcgtcttcgc ggagggcggc ctgctgggcg ccggccggca gaccacggcc
     7861 tcgctgtaca tgttctggca tgccggcttc ccgctgttcg tgctggccta caccgtcctg
     7921 aagcagcgcg agcgccagga cgccacggcg gccgcggcca acgagcaggc ctggttcgcg
     7981 cccgcccgcc cgcacgaccg cgtcgcgctg gccctgcccg tcgccaccgt cgccctggcc
     8041 gcgctgctga cgctgtacgc caccgtcggc caggcctggc tgcccacctt cctctacccc
     8101 ggccacttcg gcctggcgct gaccttcagc gctgcggcga tctgggcgct gagcctggcg
     8161 ggcgtggccg cgctctggcg ccaccggccg cattcggtgc tggacctgtg gctcagcgtg
     8221 tcgctggtgg cgtggtcgtt cgacgtcgcg ctgtcgtcgc tgctcaacgc gcagcgcttc
     8281 gacttcggct tctatgccgg ccgcgtatac ggcctgatcg cgggcagcgc ggtgctgatc
     8341 gagctgctgc tggagaacgg cgcgctctat gcccgcctcg tgcgcgtcca ccacaacgac
     8401 cgccgccagg ccgccgcgct gcgcatcgcg cgcgacgagg cgcgttcggc cgaccaggcc
     8461 aagggcctgt tcctggccaa catgagccac gagatccgca cgcccatgaa cgcggtgatc
     8521 ggcctgacgg cgatggtgct gcagacccgg ctcgacgaga cgcagcgcga ctacctgtcc
     8581 aaggtgcaga cgtcgtccaa ggccttgctg acgctgctca acgacatcct ggactactcc
     8641 aagatcgagg ccggcgcggt gtcgctggag gccgaggagt tcagccccga ggagacgatc
     8701 gagaacgtgg gcaacctgtt ctcggccaag gtggaggaag cccggctgga cctgctgttc
     8761 gagatcgacc gcgacatgcc gccgcgcctg gtcggcgacg ccttgcggct ggcccaggtg
     8821 ctcaacaacc tggtgggcaa cgcgatcaag ttcacgtcgg tcggcgaggt ggtgatcggc
     8881 gcgtgcgtgg tctcgcgctc cgacgagcag gtcgagctcg cgttctccgt gcgcgacacc
     8941 ggcatcggcg tcacgcccga gcaggccgag cgcctgttca acgtgttcca gcaggccgag
     9001 cgttccaccg cgcgcaagta tggcggcacc ggcctgggcc tggcgatctg caggcggctc
     9061 gtggaactga tgggcggccg catcgaggtc acgcccgcgc cgggcggtgg cagcatcttc
     9121 ggcttcacct gcaagttcgc catcgcacgc ggcgccagcg agcgcatcga cctgcaccgc
     9181 atccgcggca tgcgcacgct ggtggtcgac ggccagccca ccgagcggct gatcctgcag
     9241 cagatgctcc agagctggcg cttccaggtc ggcaccgcgt cctttcccga cgacgcgctc
     9301 cacaagctgc gccgcgccga cccgagcgcg ccgcacgagc tgatgctgct cgattggaag
     9361 acgggcgacc tcgacctgct cctgaaggca cgccgcgccg tggaggagca ccgcagccag
     9421 cccggcgcgg cgcccctgtc ggtgatcgcg atgacctcgc tggcggcgcg cgaacgcgtg
     9481 gccgaatcgc tcaagggcct gccgggcgtg gacatcgtca ccaagcccgt gacgccgtcg
     9541 cgcctgttcg acaccgtcgt gcgcctgcag cacggcgagg ccgctccgac gccccgcacg
     9601 ggcgagcggc agttcgacct cgaggagtcg atgcggccga tccgcggcgc gcgggtgctg
     9661 ctggccgagg acaacccggt caaccagcaa gtggccacgg ccttcctgca ggccggcggg
     9721 ctggtcgtga ccttggccga gaacggcgtc gaggcggtcg agtgggtcag gaaggcgcac
     9781 ttcgacgtcg tgctgatgga catgcagatg cccgacatgg acggcacgca ggccacgcgc
     9841 ctgatccgca agctgcccca gggcatgggc ctgcccatca tcgcgatgac cgccgcggcg
     9901 atggactccg accgccagga gtgcctggcc gccggcatga acgcgcacgt cgccaagccg
     9961 atcgacgccg agaagctggt gaggacgctg ctcgaatggg tgtcgccgcg cgggatcgcg
    10021 cgcgggcgcc ccgcgagctg aagacgtcta acggggcaac gcgtacgcga tcacgaagtc
    10081 gcccggcttg gtgccggtcg atccgtgccc gccggcgatg accagcaggt actgcctccc
    10141 atcggccccc aggtaggtca tcggcgtggc ctggcctccc gccggcaggc ggtcttccca
    10201 cagctggcgg ccggtgcgca cgtcataccc ccgcacgtag tcgtccatgc tgcccgagag
    10261 gaacgcgacg ccgccacccg tcatcaccgg gccgccgagg ttggggacgc ccatgcgcaa
    10321 tggcagcggg atcggcgaca ggtcgcgcac ggtgccgttg cggtgctccc aggcgatcct
    10381 gccggtgcgc aggtcggcgc cggccacgta gccccagggc ggcgcctggc acgggatgcc
    10441 cagcggggac gtgaacggat gcagcttgac ggcgaacggc gcgccgaaat tctcgttgag
    10501 cgccggcagc gcgccctgcg ggggcttgcc gttctgcacg agctcgacgg tcgggttcgg
    10561 gcgcggcacg agcgtcgaca cgaaggccag gtaggtcggc gtcgtgaacg ccacctggcg
    10621 ctgcggatcg accgcgacgc cgccccagtt gaacgtgccg aagttgcccg ggtagatcag
    10681 cgccccctgc gtcgacggcg gcgtgaagcg gccctcgtag cgcagcttgt gcagcgcgat
    10741 gcggcacatc atctggtcga tgatcgtgcc gccccacatg tccttgccgg ccagcttcgg
    10801 cggctcgtac gacagcgcgg acttcggctg cgtcggggcg ctgtggtcgc ccgcggcggc
    10861 accctggggc gcgggcacct cggtcacggg cagcagcggc tggccggtgc ggcgatcgag
    10921 cacgaacagc tcgccctgct tggtcggctg caccagcgcg gggacggtcg tgccgttgac
    10981 ggtgaggtcg accagcgacg gctgcgacgg cacgtcgtag tcccacaggt cgtggtgcac
    11041 cgtctggaac acccagcgca cccggccggt ggcgaggtcc agcgcgacga ccgacgagga
    11101 gtatttctcg acggccgggc tgcgccggcc gccccactgg tcgggcggct ggttgcccat
    11161 cggcaggtag accagcccca gcgcctcgtc gacgctgccg atcgaccagc tgttgggcga
    11221 attggcggtg taggtggcgt ccgcggcgat cggcgtggtc acatccgggt tgccggagtc
    11281 ccagttccac acgagggcgc cggaatcgat gtcgaaggcg cggatgacgc cggactcctc
    11341 cttggtcgag aagttgtcca gcaccgtgcc gccgacgatc accagcttct tcgtgacgat
    11401 caccggcgag gtcgagtagt acgagcccgg attcaggttg ggcatgttgg cgcccaggtc
    11461 gatctgcccc gcgccagcgc cgaagcgcga gcagacggcg ccggtctcgg ggtcgagggc
    11521 gatcaggcgc ccgtcggcgg tggccaggaa cagctttgca ccgcagtcgg cgcgtgcctt
    11581 gccgtcgttg gcggcgatgt ccaggtgccg gcccgacgtg cgcagcgcca cggaggcggc
    11641 cggcgcgggc gccgcggcgg cctcgggtgg cggctcgtag gacaggccgc ggcaggtctg
    11701 gtgctgcagc gcgaggcccg tctccatctt cgggtcgtag cgccaggcca gccggcccgt
    11761 ggtggcgtcc agcgcgatca ccgactggtg cggcgtgcac aggtacagcc ggtcgccgat
    11821 cttcagcggc gtcacctcgt cggtggtctc ggtcgggtcg cccggccggc cgcggacatc
    11881 gccggtgcgg aactgccatg cgaccttcag cctgtcgacg ttgccgggcg tgatctgcgc
    11941 cagcggcgac cagcgctcgc ccgcggccgt gcgcccgtag gcatgccact cgccgtcggg
    12001 cacgccgctg gccgccaccg cggtaccctg cccggcctcc gccaactgcc ccgcgatctc
    12061 atgggtgtcg cgcaggcccg aaccgatggc gaccacggcc gacacgccca cgacgagcgc
    12121 cagcacgatg cggcctgacc gcggcccgcg caagtggcgg gagatccacg gcagcaccag
    12181 cagcgcgccg aacacgaacg cgaggtccag ccgcgcggcc aacggccacc agtccaggcc
    12241 cacctcccac aggctccagg cgagcatcgc ggccagcagc acggcgaaca cgatgagcgc
    12301 ctcggcctgc ccgcgccaaa gcagcacgcc cgcggcgccg aagccgatcc cggccagcag
    12361 gtagaacggc gagccgccgg ccgtcgcgag gacgacgccg ccgacgatca gcgccagtcc
    12421 gacgaggatc agcaggacac cgaccacgac gaaccggacc aggggacgaa ctgtggccat
    12481 cgcggccctc ctcggaatct gggatccgcc gaccgtggcg gccggtggcc gcggcagccc
    12541 gcgtgcccgg tcgaggacga accggcgact caggccgact ggcgcaccac cagccagacg
    12601 aaggcggcgc cgcagcaggt cagcgcgatc agcttgaccg ccacgccggg accggcacgc
    12661 gagtgcgcct cgggcaggat gtccgagaca ccgatgtaca gcaggaagcc ggcgaagaag
    12721 ccgaggtaga ccatcaactg gaatggcggg atcgtgaagg ccagcgtcag cagcacgccc
    12781 agcagcggcg cc
//
LOCUS       DATCRO010000068         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_698377, whole genome shotgun sequence.
ACCESSION   DATCRO010000068 DATCRO010000000
VERSION     DATCRO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698377"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2014
                     /gene="dnaG"
                     /locus_tag="VIN75_01895"
     CDS             17..2014
                     /gene="dnaG"
                     /locus_tag="VIN75_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8972941.1"
                     /translation="MIPAAFIQELLARVDIVEVVGQHVELKKAGINHKGLCPFHGEKS
                     PSFIVSPTRQTYHCFGCGVHGDAIRFLVEQHGMGFVDAVTDLAQQVGMPVPEDNASPE
                     ERAEAARKKAQVTTLTDLLAKAADHYKQQLKGSPRAIDYLKGRGLSGEIAARFALGFS
                     PDGWRTLASCFPRYDDPLLVESGMVIVSGDDPATQKRYDRFRERIMFPIRNVKGEVIA
                     FGGRVLDKGEPKYLNSPETPVFSKGRELYGLFEARQGLRDKGYVLVTEGYMDVVALAQ
                     LGFPNAVATLGTACTADHVHKLLRFTDKVIFSFDGDAAGRRAAGRALEAALPHATDTR
                     SFRFLFLPKEHDPDSYVREHGAKAFEQYVQDALPLSQQLVEVAQHDCDMATAEGRARM
                     LAQARPLWSALPEGALQMQLLGELAIKAGLPLADLQTLWKTDAPRARRPARDRDADDG
                     APWHDVPPDLDEANAQASGAGQHHEHTPREGQGRFQGKPRRFDNRPMNPKWAARQREE
                     AELEAQRRQPRSAPRTAESRAVQMLFGHAEYWERLTPDDHELLHGLPAPYGPLVAWLE
                     RDHSEHGPRPWAVLSHALRDDPELGPEALAIADGDAEPGATFEDFRRNVDKILDRELR
                     AQTQALITRAATDPDAMRRYREVFKHWEEVKARLSAQRAEE"
     gene            2377..>3099
                     /locus_tag="VIN75_01900"
     CDS             2377..>3099
                     /locus_tag="VIN75_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY8972942.1"
                     /translation="MTAKKSADTAKTTAAAKAAADKPVVEKKAVSAAAAKAAVKAPAA
                     KAEKAAPAAKTAAKPTKAAAKTAAPEAKGKKGKKDEAAAKPSFEDDADVEADLEGEVE
                     ADGGDEVVEDAGEVKAKVKPLRMKVSRAKERALMREFGLDETQLTEEEVAKRRHELKT
                     LIKMGKTRGFLTHQEINDHLPEKLVDNEILEAIVSMLNDMGIAVYEQAPDAATLLISG
                     QTQSGATEEEAEEAAEAALSTVD"
BASE COUNT          553 a         1106 c         1044 g          396 t
ORIGIN      
        1 tcgatagact gcgcgcgtga tcccggccgc cttcatccag gaactgctcg cgcgcgtcga
       61 catcgtcgag gtggtcggcc agcacgtcga gctgaagaag gccggcatca accacaaggg
      121 cctgtgcccg ttccacggcg agaagtcgcc cagcttcatc gtcagcccca cgcgccagac
      181 ctaccactgc ttcggctgcg gcgtgcacgg cgacgccatc cgcttcctgg tcgagcagca
      241 cggcatgggc ttcgtcgacg ccgtgaccga cctggcccag caggtgggca tgccggtgcc
      301 cgaggacaac gcctcgcccg aggaacgggc cgaggccgcg cgcaagaagg cgcaggtcac
      361 gacgctgacc gacctgctgg ccaaggccgc cgaccactac aagcagcagc tcaagggctc
      421 gccgcgcgcc atcgactacc tcaagggccg cggcctgagc ggcgagatcg cggcgcgctt
      481 cgcgctcggg ttctcgcccg acggctggcg gacgctggcc agctgcttcc cgcgctacga
      541 cgacccgctg ctggtcgagt cgggcatggt gatcgtctcg ggcgacgacc cggccacgca
      601 gaagcgctac gaccgcttcc gcgagcgcat catgttcccg atccgcaatg tcaagggcga
      661 ggtgatcgcc ttcggcggcc gcgtgctcga caagggcgag cccaagtacc tgaattcgcc
      721 cgagacgccc gtcttcagca agggccgcga gctctatggc ctgttcgagg cgcgccaggg
      781 gctgcgcgac aagggctacg tcctggtgac cgagggctac atggacgtgg tcgcgctggc
      841 gcagctgggc tttcccaacg cggtcgccac gctgggcacc gcctgcacgg ccgaccacgt
      901 gcacaagctg ctgcgcttca cggacaaggt gatcttcagc ttcgacggcg acgccgcggg
      961 ccgccgggcc gccggccgcg cgctcgaggc cgccctgccc cacgccaccg acacccgcag
     1021 cttccgcttt cttttcctgc ccaaggagca cgatcccgac agctacgtgc gcgaacatgg
     1081 cgcgaaggcc ttcgaacagt acgtccagga cgcgctgccg ctgtcgcaac agctggtcga
     1141 ggtggcgcag cacgactgcg acatggccac cgccgaaggc cgcgcccgca tgctggcgca
     1201 ggccaggccg ctgtggagcg cgctgcccga aggcgccctg cagatgcagt tgctgggcga
     1261 gctggcgatc aaggccggcc tgccgctggc cgacctgcag acgctgtgga agaccgacgc
     1321 cccgcgcgcc cgccggcccg cgcgcgaccg cgacgcggac gatggcgcgc cctggcacga
     1381 cgtcccgccc gacctggacg aggccaacgc ccaggcgagc ggcgccgggc agcaccacga
     1441 gcacacgccg cgcgagggcc agggccgctt ccagggcaag ccccgccgct tcgacaaccg
     1501 gcccatgaac ccgaaatggg cggcgcgcca gcgcgaggag gccgagctgg aggcccagcg
     1561 ccgccagccg cgttcggcgc cccgcacggc cgagagccgg gccgtgcaga tgctgttcgg
     1621 ccacgccgag tactgggagc ggctgacgcc cgacgaccac gagctcctgc acggccttcc
     1681 ggctccctac ggcccgctgg tcgcctggct ggagcgcgac cattcggagc acggcccacg
     1741 cccgtgggcc gtcctcagcc atgccctgcg cgacgacccg gagctcgggc ccgaggcact
     1801 ggcgatcgca gacggcgatg ccgagcccgg cgcgaccttc gaggacttcc gccgcaacgt
     1861 cgacaagatc ctcgaccgcg agctgcgcgc gcagacgcag gcgctgatca cccgggccgc
     1921 caccgatccg gatgccatgc ggcgctaccg cgaggtcttc aagcactggg aagaggtcaa
     1981 ggctcgactg tcggcccaac gtgccgaaga gtgagtgagg tacgccacgc ggcccgcttc
     2041 gccgggaccg tcatggcggt tttcactggc atttgaggta taatccgata tcccactcag
     2101 cggcaacttg tgacgtgcag tgagagcaac tgactccggg ccccggccac cctcgacaag
     2161 ggcatcttca ccggccacct tcctcccgca agagttgctt cttcaaacca cgttcacgtc
     2221 actttgccag ccggtgtccc gtcgtgtcga ccgcgaaggg aaattcatcg cttgccttct
     2281 ctttcagctg cttgcccgta tatatcacgg gccagtcgaa attgacgcgg gcgacgccgc
     2341 cgggcggcct ggggccacat tcattcaagg aaacgcatga ctgccaagaa gagcgctgat
     2401 actgccaaga cgaccgctgc cgccaaggcg gccgccgaca agcctgtcgt cgagaagaag
     2461 gcggtgtccg ctgctgccgc gaaggcggcc gtgaaggcgc cggcggccaa ggccgagaag
     2521 gcggcgcccg cggccaagac cgcggccaag cccaccaagg ccgccgccaa gacggccgcg
     2581 ccggaggcca agggcaagaa gggcaagaag gatgaggccg ccgccaagcc gtcgttcgag
     2641 gacgacgcgg acgtcgaggc cgatctcgag ggcgaggtcg aggccgacgg tggcgacgag
     2701 gtcgtcgagg acgccggcga ggtcaaggcc aaggtcaagc cgctgcgcat gaaggtctcg
     2761 cgcgccaagg agcgcgcgct gatgcgcgag ttcggcctgg acgagacgca gctgaccgaa
     2821 gaggaagtcg ccaagcgccg ccatgagctc aagacgctca tcaagatggg caagacgcgt
     2881 ggcttcctga cgcaccagga aatcaacgac cacctgcccg agaagctggt cgacaacgag
     2941 atcctcgaag ccatcgtgtc gatgctcaac gacatgggca tcgccgtcta cgagcaggcg
     3001 cccgacgccg ccacgctgct gatctcgggc cagacccagt ccggcgcgac ggaagaggaa
     3061 gcggaagaag cggccgaggc cgcgctgtcg accgtcgac
//
LOCUS       DATCRO010000069         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_147084, whole genome shotgun sequence.
ACCESSION   DATCRO010000069 DATCRO010000000
VERSION     DATCRO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147084"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..412)
                     /locus_tag="VIN75_01905"
     CDS             complement(104..412)
                     /locus_tag="VIN75_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972943.1"
                     /translation="MDSKQLFIEHLAGELQAMEVGRVPMNALRYRVHAKMLRKALAEA
                     STPSLLERQGGAHPQVVHALSNRFFEETGHLLQPNATAHKARSVLERTMAACRQARQK
                     "
     gene            complement(579..1493)
                     /locus_tag="VIN75_01910"
     CDS             complement(579..1493)
                     /locus_tag="VIN75_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8972944.1"
                     /translation="MIKPATAWSLARETVSSWSDDYAASMGAALAYYTMFSIAPLLLI
                     VISLAGLFFGEQAARGEILDQLEGLMGAEGAHAVQALLASVNQPKAGILATLLGVGAL
                     VIGATTVIGELQSALDRIWRAPRRSSEGGIAKWIHSRLTSLGMIMGIGFLLMVSLLAS
                     AALAAVQRWFGRWIDLGGLASAVDFVVSFGFITVAFAMIYKLVPRVRVEWRDVWIGAV
                     VTSLLFTVGKMAIGLYIGRSAVASTFGAAASLVAMVVWVYWSAQIFLLGAEFTWVYAR
                     RCGSLRDRADVLPDAGPPAPRARSDDPR"
     gene            complement(1573..2028)
                     /locus_tag="VIN75_01915"
     CDS             complement(1573..2028)
                     /locus_tag="VIN75_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEY8972945.1"
                     /translation="MDNQDTIDVLNTLIETCNDGERGFRNVAEHVHSADLKTLFATRA
                     NECRQACADLRAQVLQLGGDPETGGSASGALHRGWVSLLGTLSGHSDKSLLEACERGE
                     DSALERYRDALDQALPDNIRMLIERQYEGVKRNHAQVRSLRDQARANAS"
     gene            complement(2145..3425)
                     /locus_tag="VIN75_01920"
     CDS             complement(2145..3425)
                     /locus_tag="VIN75_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8972946.1"
                     /translation="MSAPVSLRLSTPPKLVSGPAPQSQLMALHSHAHALVAGLVEPLV
                     TGNRVDLLQDGPCTYSAMFEAIEQARDHVNLESYMLEAEGPGQEFAQRLIARAKAGVR
                     VNVLYDSFGSIGTPESYFEHLRANGVNVCEYNPLRRLGNLLSRALHLRDHRKLMVVDG
                     RIGFIGGVNISPVYAAGSSPLAALAGSGGVDAQEAAAPGWRDTHVRVEGPIVAQLQRL
                     FMQHWSRHSSIALRGTHYFPPLGPAGQQRVALAACDAGRRRNPFYRALLSALDAAQHR
                     IYLTTAYLVPTRRFMRTLLRAVQRGVEVRLLLPGTSDFWLPLQAGRSHYGRLLRGGVH
                     IHELQDALLHAKTCVIDGLWTTVGSSNLDWRSFLHNAEANLVVLDAGLAQEMEQVFNA
                     DIARSREIVRAQWSRRGWRQRLAETVARRFEFFL"
BASE COUNT          541 a         1225 c         1310 g          495 t
ORIGIN      
        1 ggccgtcgcc ggggcggcgt gcgcggccag gaacagggcg gcaaacgcga ggatcgccgg
       61 gatgcgaacg gccacgacgg ctcctcggtc gggggcgccg gacttacttc tggcgagcct
      121 ggcggcaggc ggccatcgtg cgttccagca ccgagcgcgc cttgtgcgcc gtggcgttgg
      181 gctgcagcag gtggccggtc tcctcgaaga agcggttcga cagcgcgtgc acgacctgcg
      241 ggtgggcgcc gccctggcgc tccagcagcg acggggtgga ggcttcggcc agcgccttgc
      301 gcagcatctt ggcgtgcacc cggtagcgca gcgcgttcat ggggacgcga cccacctcca
      361 tggcttgcag ttcgcccgcg aggtgttcga tgaaaagctg tttcgagtcc atgaccggag
      421 catgacatcc gaggcgtcgg cccatcggag gaacaccggg cgcaaacgtg cgtttttccc
      481 gcccggacgg ggaaatctgg cgcccaatgt cgcgttggaa cgcgattgcg gcgacagctc
      541 gttacaccgc ttgcggccag acaagcctgg acggcagatc agcgcggatc gtccgagcgg
      601 gctcgcggcg ccggcgggcc ggcgtcgggc aggacgtcgg cgcgatcgcg cagcgaaccg
      661 cagcgccgcg cgtagaccca ggtgaactcc gctccgagca ggaagatctg cgccgaccag
      721 tagacccaca ccaccatcgc caccagcgag gccgcggcgc cgaaggtcga cgccacggcg
      781 ctgcgcccga tgtacagccc gatcgccatc ttgcccacgg tgaacagcag cgacgtcacc
      841 accgcgccga tccacacgtc gcgccactcg acgcgcaccc gcggcaccag cttgtagatc
      901 atcgcgaacg ccacggtgat gaagccgaag ctgacgacga agtcgaccgc cgacgcgagc
      961 ccgcccaggt cgatccagcg gccgaaccag cgctgcaccg cggccagcgc ggcgctggcc
     1021 agcagcgaga ccatcagcag gaagccgatg cccatgatca tgcccagaga ggtcaggcgc
     1081 gagtggatcc atttggcgat gccgccttcg ctggaacggc gtggcgcgcg ccagatgcgg
     1141 tcgagcgcgc tctgcagctc gccgatcacc gtggtggcgc cgatcaccag cgcgcccacg
     1201 cccagcagcg tggcgaggat gccggccttg ggctggttga cgctggccag cagcgcctgc
     1261 accgcgtgcg cgccctcggc gcccatcagg ccttcgagct ggtcgaggat ctcgccgcgc
     1321 gcggcctgct cgccgaagaa cagccccgcc agcgagatca cgatcagcag cagcggcgcg
     1381 atcgagaaca tcgtgtagta ggccagtgcc gcgcccatgc tggcggcgta gtcgtcgctc
     1441 cacgaggaca cggtctcgcg cgcgagggac caggcggtgg cgggtttgat cacgcggtcg
     1501 gcctccggct tgcaaacgac agcgcccgca cgaggcgggc gcgccgggac ggcgggcggc
     1561 ccccgcccgc ggtcaggacg cgttcgcgcg cgcctggtcg cgcaggctgc gcacctgcgc
     1621 gtggttgcgc ttgacaccct cgtactggcg ctcgatcagc atgcggatgt tgtccggaag
     1681 ggcctggtcc agcgcgtcgc gatagcgttc cagcgcggaa tcctcgccgc gctcgcaggc
     1741 ctccagcagc gacttgtcgc tgtggcccga cagggtgccc agcagcgaga cccagccgcg
     1801 atggagcgcg ccgctggcgc tgccgccggt ctcggggtcg ccgcccagct gcagtacctg
     1861 cgcgcgcagg tccgcgcagg cctggcggca ctcgttggcg cgggtggcga acaaggtctt
     1921 caggtcggcc gaatgcacgt gctcggcgac gttgcgaaag ccccgctcgc cgtcgttgca
     1981 ggtctcgatg agggtgttca ggacatcgat ggtgtcttgg ttgtccatgg gaaggccttt
     2041 caggttgcca ggaagtggcc ctccagcggc agatcgtgtg cccatcgcgc gggctcgcgg
     2101 cgaccggcaa agcctgctgg tccgtgccgc gccgtccgcg cgggtcagag gaagaactcg
     2161 aagcggcgcg ccaccgtctc ggccaggcgc tggcgccagc cgcggcgcga ccactgcgca
     2221 cgcacgatct ccctggagcg cgcgatatcg gcattgaaca cctgctccat ctcctgcgcg
     2281 agacctgcat cgagcacgac gaggttggcc tcggcgttgt gcaggaagct gcgccagtcg
     2341 aggttgctcg agcccaccgt cgtccacagg ccgtcgatga cgcaggtctt ggcatgcagc
     2401 aaggcgtcct gcagttcgtg gatgtgcacg ccgccccgca gcagccgccc gtagtgcgag
     2461 cggccggcct gcagcggcag ccagaagtcg ctcgtgccgg gcagcagcag gcgcacctcg
     2521 acgccgcgct gcaccgcgcg cagcagggtg cgcatgaagc ggcgcgtggg caccaggtag
     2581 gccgtggtca ggtagatccg gtgctgcgcg gcatcgagcg ccgacagcag cgcgcggtag
     2641 aacgggttgc gccggcgtcc cgcgtcgcag gcggcgagcg ccacccgctg ctggcccgcc
     2701 ggcccgagcg gcgggaagta gtgcgtgccg cgcagcgcga tcgaggagtg ccggctccag
     2761 tgctgcatga acagccgctg cagctgcgcc acgatcggcc cctcgacgcg cacgtgggtg
     2821 tcgcgccagc cgggtgcggc cgcctcttgc gcgtcgacgc cgcccgagcc cgcgagtgcc
     2881 gccagcggcg acgagccggc cgcgtacacg gggctgatgt tgacgccgcc gatgaagccg
     2941 atgcgcccgt cgaccaccat cagcttgcga tggtcgcgca ggtgcagcgc gcggctgagc
     3001 aggttgccca ggcggcgcag cgggttgtat tcgcacacgt tgacgccgtt ggcgcgcagg
     3061 tgctcgaagt agctctcggg agtgccgatc gagccgaagc tgtcgtacag cacgttgacg
     3121 cgcacgccgg ccttggcgcg cgcgatcagc cgctgcgcga actcctggcc ggggccttcg
     3181 gcctccagca tgtagctctc gagattgacg tggtcgcgcg cctgctcgat ggcctcgaac
     3241 atcgccgagt aggtgcaggg accgtcctgc agcaggtcga cgcggttgcc ggtgacgagc
     3301 ggttcgacca ggccggcgac gagcgcatgc gcatggctgt gcagggccat gagctgcgac
     3361 tgcggcgcgg ggccggacac gagcttcggc ggcgtggaca accggagcga gacgggcgcg
     3421 gacatgaggc aattccttcc cgggttcggc tgcgcgcggg gtggcgcaat gccggaggcg
     3481 gggacggtcc ctttggcaat ggccatgccc gggacgcgtc ctccgaggga aagcgagggt
     3541 cgcgagggtg cgtgcggggt ggcatcccgc c
//
LOCUS       DATCRO010000070         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_507178, whole genome shotgun sequence.
ACCESSION   DATCRO010000070 DATCRO010000000
VERSION     DATCRO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507178"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VIN75_01925"
     CDS             <1..492
                     /locus_tag="VIN75_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY8972947.1"
                     /translation="ALPRVAELQTQGGITAAEAWAWHHARLAERGDRYDPRVALRIRR
                     GAAITPDAYQALLDARQRWIHDMTTSMHGTEVFLSPTVPLQAPEIAPLLHSDDLFFAT
                     NARLLRNTAVVNLLDGCAISIPCQHPGELPVGLMLWAPASQDAAVLGAAARVEAALAV
                     GAR"
     gene            494..1699
                     /locus_tag="VIN75_01930"
     CDS             494..1699
                     /locus_tag="VIN75_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8972948.1"
                     /translation="MRIAIIGAGLAGVTSAYELALQGHEVQVFERDTSVATGASFAPP
                     GLIAPGLMAAGANPPLRWRGKPGQLGWRFAHWRAGRARGAQAMPEALHALALRGQAVM
                     AVWRRELQMEVEREAGVLLLWRSAKDYKAAAALLARLQERQVPHRELDAEGCRAQEPG
                     LNAETPLHGGILLSQDEAANARQFSQALRVEAQRLGVRWHFNTDVLRIEPGSLPAVHV
                     AAREPVRVDAVLVCAGKGAAPLLAAQGLKLPWGTVHTHTITAPLRQLEAHPDLGPRAT
                     VVDMSLGVSLTRLGQRVRVGGAHELGGASAQPDGQSMGALHQAAHDWFPGALLAGGQQ
                     RWKTARLLLPDELPLIGESGLPGVWLNIGHGEHGWTMAGAAAQLLSARLAKKDPGIDA
                     ALLEPGRLR"
     gene            1777..3315
                     /locus_tag="VIN75_01935"
     CDS             1777..3315
                     /locus_tag="VIN75_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8972949.1"
                     /translation="MRPVLPLSRAQPLHDVVSSRHIESAALSRHAPHALIERAGLAVA
                     RLALALRPLGRHAVILAGPGNNGGDGLIAARHLAARGWRVDLRLVGAGPTPGTDAAHA
                     LDSARAAGLAPLTDLGTMPRDADLVIDALLGLGASRAPEGAFTEAIRAVEALRRAGAL
                     VLSVDLPSGLDARTGQPLGADVVEADHTLTFLTLKSGLFTGEGRALAGRLWFDDLGEP
                     QGDGPGGDAAAHAPALLFGRERIARWQRDFPRSASSHKGRHGDVVVLGGAPTMRGAAW
                     LAATSALAAGAGRVYAALPDDDGLAWPARPELMHWDFARWSAPADAWRDVVAVCGCGG
                     GTAIAPALSTVLHGARRVVLDADALNLVAADEALQAALCRRATHGWPTLLTPHPLEAA
                     RLLGFDSAADVQADRLAAAAQLAARFGATVVLKGSGSVVATPQEPLSINSTGSSALAT
                     AGSGDVLAGWIGGMWAQSPEIPPHALATAAVAWHGAAAEGHAGPMLAADLVAAMAALH
                     AGAA"
     gene            complement(3422..>3722)
                     /locus_tag="VIN75_01940"
     CDS             complement(3422..>3722)
                     /locus_tag="VIN75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972950.1"
                     /translation="DKRAEQRARREAAAAPAATVEPMPLPPPDPPTAADALERIEQAD
                     RVLKAERRKSERATKGAAAHPVKAAKRTARSAAKTVAAKGSGAPAAKTARRKRGA"
BASE COUNT          450 a         1389 c         1378 g          505 t
ORIGIN      
        1 gccctgcccc gtgtggcgga actgcaaacc cagggcggca tcaccgccgc cgaagcctgg
       61 gcatggcacc atgcgcgcct cgccgaacgc ggcgaccgct acgacccgcg cgtcgcgctg
      121 cgcatccgcc gcggcgcggc gatcacgccc gacgcctacc aggcactgct cgacgcgcgc
      181 cagcgctgga tccacgacat gacgacctcg atgcatggca ccgaggtctt cctcagcccc
      241 accgttcccc tgcaagcccc ggagatcgcc cccttgctgc acagcgacga cctgttcttt
      301 gccaccaatg cgcgcctgct gcgcaacacc gccgtcgtga acctgctcga cggctgcgcg
      361 atctcgatcc cctgccagca tcccggcgaa ctgccggtcg gcctgatgct gtgggcgccg
      421 gcgtcgcaag acgccgccgt gctgggcgcg gccgcgcgcg tcgaggccgc cctggcggtg
      481 ggagcgcgct gagatgcgga tcgcgatcat cggcgccgga ctggccggcg tgaccagtgc
      541 ctacgaactc gcgctgcagg ggcacgaggt ccaggtcttc gagcgcgata ccagcgtcgc
      601 gaccggcgcg agcttcgcgc cgccgggact catcgcgccg ggcctgatgg ccgcgggagc
      661 caacccgccg ctgcgctggc gcggcaagcc gggccagctc ggctggcgtt tcgcccactg
      721 gcgcgccggc cgcgcgcgcg gcgcgcaggc gatgcccgag gccctgcatg cgctggcgct
      781 gcggggccag gcggtgatgg cggtgtggcg gcgagaactc cagatggagg tcgagcgcga
      841 ggctggcgtg ctgctgctgt ggcgcagcgc caaggactac aaggccgcgg cagcgctgct
      901 ggcgcggctg caggagcggc aggtgccgca tcgcgagctc gatgccgagg gatgtcgcgc
      961 gcaagagccg ggactcaacg ccgagacgcc cctgcacggc ggcatcctgc tgtcgcagga
     1021 cgaggcggcc aacgcgcgcc agttcagcca ggcgctgcgg gtggaagcgc agcgactggg
     1081 cgtgcgctgg cacttcaaca ccgacgtgct gcgcatcgag cccggcagct tgcccgcggt
     1141 gcatgtcgcg gcccgcgagc cggtgcgcgt ggatgcggtg ctggtgtgcg caggcaaggg
     1201 cgcggccccg ctgctcgccg cacagggcct gaagctgcct tgggggacgg tccacaccca
     1261 cacgatcacg gcgccgctgc ggcagctgga agcgcatccg gacctggggc cgcgcgccac
     1321 cgtcgtggac atgagcctcg gcgtcagcct gacacggctg ggacagcgcg tacgcgtcgg
     1381 cggcgcccac gagctgggcg gcgcgtcggc gcagcccgac ggccaatcga tgggcgcgct
     1441 gcaccaggcg gcgcacgact ggttccccgg cgccctgctg gctggcggcc agcagcgctg
     1501 gaagactgcg cggctgctgc tgcccgacga gttgccgctg atcggcgaat ccggcctgcc
     1561 gggcgtctgg ctcaacatcg gccatggcga gcatggctgg accatggccg gcgccgcggc
     1621 gcaactgctc tcggcacggt tggcgaagaa ggatcccggc atcgacgcgg cactgctgga
     1681 gcccggccgg ctgcgctgat cacgccgccg acgagaaccg gtcgtgcggt ggtcccgcgc
     1741 gccgcttcgc ccgctcgaag cgatagagtc ggcgccatgc ggcccgtcct ccctctttct
     1801 cgcgcgcagc ctctgcacga cgtcgtgtcg agccgacaca tcgagtcggc tgcgctctcg
     1861 cgccatgcac ctcacgcgct gatcgaacga gccggcctgg cggtcgcccg actcgcgctg
     1921 gcgctgcgtc cgttgggccg gcacgcggtg atccttgctg gccccggcaa caacggcggc
     1981 gacggcctca tcgcggcccg gcatctcgcc gcacgcggat ggcgggtcga cctgcgcctc
     2041 gtcggggccg gaccgacacc cgggaccgac gccgcccatg cgctcgacag cgcacgcgcc
     2101 gccggcctgg cgccgctcac cgacctcggc acgatgccgc gcgatgccga tctggtcatc
     2161 gacgccctcc tcggcctggg cgcgagccgc gcgcccgagg gcgcattcac cgaagcaatc
     2221 cgggctgtcg aggccctgcg ccgcgccgga gccctggtgc tgtcggtgga cctgccgagc
     2281 gggctcgacg cacgcaccgg ccagccgctg ggcgccgacg tggtcgaggc cgatcacacg
     2341 ctgaccttcc tgacgctcaa gagcggactg ttcaccggcg aggggcgcgc gctcgccggt
     2401 cgtctctggt tcgacgacct cggcgagccg cagggcgatg ggccgggcgg ggacgccgcc
     2461 gcgcatgcgc cggccctgct gttcggccgt gagcgcatcg cgcgctggca acgcgacttc
     2521 cctcgctcgg cctcgagcca caaggggcgg catggcgacg tggtcgtgct cggcggcgct
     2581 ccgacgatgc gcggcgccgc ctggctcgcg gccacgtccg cgctcgcggc aggcgctggg
     2641 cgggtctatg cggcgctgcc ggacgacgac ggcctggcct ggccggcgcg tccggagctg
     2701 atgcattggg acttcgcgcg ctggagcgct ccggctgacg cctggcgcga cgtcgtcgcg
     2761 gtgtgcggct gcggcggcgg cacggcgatc gcgccggcgc tttcgaccgt gctgcatggc
     2821 gcgcgccgtg tcgtgctcga tgccgacgcg ctgaatctcg tggccgccga cgaagcgctg
     2881 caggcggcgc tgtgccgccg cgccacgcat ggctggccga ccctcctgac gccccatccg
     2941 ctggaagccg cccgtctgct cggattcgac tcggccgccg atgtgcaggc cgatcggctc
     3001 gcggccgccg cgcaactggc agcgcgcttc ggcgcgacgg tggtgctgaa gggctccggc
     3061 agcgtcgtcg cgacgccgca ggagccgctg tcgatcaaca gcaccggctc gtcggcgctg
     3121 gcgacggcgg gcagcggcga cgtgctggcc ggctggatcg gcgggatgtg ggcccagtcg
     3181 ccggagatcc cgccgcacgc gctcgccacc gcggccgtcg cgtggcacgg tgcagccgcg
     3241 gaaggtcatg ccgggccgat gctcgccgcg gatctcgtgg cagcaatggc cgcgttgcac
     3301 gctggggcgg cctagcgcgc caacgctgcg ttccaccacg cgggccgccg ggccttcagt
     3361 gacgaaggcc tggcaatgcc aggccttctc cgtggtgccg cttgaagcgg gcgatcgccc
     3421 gtcaggcgcc ccgcttgcga cgcgccgtct tggccgcggg ggcaccgctg cctttcgcgg
     3481 cgacggtctt cgccgcactg cgcgccgtgc gcttggctgc cttgactgga tgcgccgccg
     3541 cacccttggt cgcgcgctcc gacttgcgac gctcagcctt cagcacgcga tcggcctgct
     3601 cgatgcgctc gagcgcgtcg gccgccgtgg gcggatccgg cggcggcagc ggcatcggct
     3661 cgaccgtcgc ggcaggcgcg gcagcggctt cacgccgtgc acgctgctcg gcgcgcttgt
     3721 cc
//
LOCUS       DATCRO010000071        16353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_180784, whole genome shotgun sequence.
ACCESSION   DATCRO010000071 DATCRO010000000
VERSION     DATCRO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16353
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180784"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VIN75_01945"
     CDS             <1..696
                     /locus_tag="VIN75_01945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8972951.1"
                     /translation="SQGSMITFKRPDGQELQGYLAKPAKAEGAPGIVVIQEWWGLNDQ
                     IRGVADRLAHAGYLALVPDLYRGKTTVEREEAHHLMTGLDFGDAASQDVRGAVQFLKT
                     RAAKVGCVGFCMGGALTLLAAANVPELDAAVTWYGCPPLEYLDAGKIKVPLQGHWADQ
                     DQFFPPEQVRGLTAKLEAAGVQHDFHHYLAHHAFGNEQAVGEGRIAETHYDPVWAQRA
                     WDRTFLFFAKHLG"
     gene            902..1684
                     /locus_tag="VIN75_01950"
     CDS             902..1684
                     /locus_tag="VIN75_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY8972952.1"
                     /translation="MARPPVIVETNGWIATVTLDRPDCRNAVDGPTATLLREAFERFE
                     GDDELRVLVLTGAGGQFCAGADLKAMADPARRNAVEPTGAAAGPMGPTRLRMSKPVIA
                     AIEGHAVAGGLELALMCDLRVAARSAVLGVFCRRWGVPLIDGGTVRLPRIVGAGRALD
                     MILTGRAVPADEALAFGLVNRVVEDGGALAAAQALAAEIAEFPQACLRVDRASAMGQW
                     DLSLEDALRHEGAAGFAVVAAEGLAGAQRFAAGAGRHGAPAA"
     gene            complement(2114..6550)
                     /locus_tag="VIN75_01955"
     CDS             complement(2114..6550)
                     /locus_tag="VIN75_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8972953.1"
                     /translation="MPLQDFHPAVARWFERRFGAPTAAQVAAWPHIRAGRDTLVAAPT
                     GSGKTLTAFLAALDDLVRRGVAGALPDETAVVYVSPLKALSNDIRANLEEPLAGIRAE
                     LVSQGLPDVEIRTAVRTGDTPQRERQQAMRRPPHILVTTPESLYVLLGSLAGRRMLAN
                     VRTVIVDEIHALAANKRGSHLALSLERLQNIVNFPPCPDEPDEDGVLPDPKAATARAE
                     PRHVTRVGLSATQKPIAEVARFLVGAGGVVDGQADCAIVDIGYKKQRDLALEVPPTPL
                     SAVMSHEQWDMVYQRIVELVATHRTTLVFVNTRRMAERAARHLAELLGDDAVAAHHGS
                     LARELRLDAEQRLKRGELKVLVATASLELGLDIGDVDLVCQLGSPRSIAAFLQRAGRA
                     GHAVGGTPKARLFPQSRDELVECAALLEAVQRGELDTLHVPPMPLDVLAQQVVAETAC
                     RDWDEDALYALMRRAWPYAALPRERFDAVLRMLADGYTTRQGPRGAYLHRDAVHHQLR
                     ERRGARMTALTSGGTIPETGDYTVLLEPQSDRIGTVNEDFAVESLAGDVFQLGNTSYR
                     ILRVEPGRVRVEDAKGQPPNIPFWLGEAPGRSDELSMAVARLREAVGEQVRREGQPAA
                     IQGLVDATAGGLAADAARQIVEHLASAQAVLGALPTHRRIVMERFFDQSGGMQLVVHS
                     PFGSRLNRAWGLALRKRFCRQFNFELQAAATEDAIVLSLSTSHSFPLDEVARYLRSAT
                     AMDVLVQALLDAPLFAVRWRWNATTALALPRFVGGRKVAPQLQRMKSEDLLASVFPDQ
                     LACAENLAGPREVPDHPLVMQTMHDCLHEAMDAEGWLAVLRGIESGAIEVIARDLPAP
                     SPLAAEALTARPYAYLDDAPIEERRTQAVQARRFGDADNVGEMGALDADAIAGVREEA
                     WPRARSADEVHEALMTLGAITADEARANQHWDAALAALARAGRATRLRAGEDGLWVAA
                     ERLATLRPLWPAAEATPAITPPAGYEAPFETLQEALRELLRSRLGGLGPVTVDALVEQ
                     LRLPRGDIEAGLAALQSEGTVFQGHVTPGLETVEWCERHLLARIHRYTLKRLRREIEP
                     VEPRDFVRFLFEWQHVAGASRVSGPEALPAVLAQLEGFEAPASLWEAEVLPARVKDYA
                     SSWLDDLCTAGRTLWTRLRPLAGGTQGGGRSSLRGTPILLLPRRAAPTWSRIAAAAAG
                     EDGEALGGRAQRVLDFLEANGASFFDEMADGARLLRAELEDALAELVVRGRASCDSYA
                     GLRALLVPAAKRATAASRSRRRVALYGIEDAGRWNVVKAPQVVPDATAHAEAVEHVAR
                     TLLRRYGVVCYEMLDREAGWLPPWRELVRVCRRLEARGEIRGGRFIAGVTGEQFALPD
                     AIATMRAVRRQPPDETLVCLSASDPANLLGTVLTGPKVPRVSGSRVLYRDGLAIGTSV
                     AGQIELLVPLDPAQARAATRALALDPQLRFMEMASTEAGARA"
     gene            6719..7645
                     /locus_tag="VIN75_01960"
     CDS             6719..7645
                     /locus_tag="VIN75_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972954.1"
                     /translation="MPACSIVRLAPPALLAATLLGCASTPLPPEPPQLVTTSTVTTHS
                     VSSSTSTAAPLTREQVAAIVASPDRTDADRTNDQRRHPVDLMTFVGARPGWTVLDVST
                     GGGYTTELLSRMVGPTGMVWAQSRPRNPDAPAARPAQPEGGAPAPTAAPAAPSRGPAE
                     ILAGRSDRMKAAGVAAAPIAFVALPFDDPVPPEVADGKLDLVTLMFNYHDFGWIPVDR
                     ASMNRAIFAALKPGGLYVIADHAGRPGTGISESGTLHRIEESFLRREVEAAGFRLLAE
                     GDFLRNPDDPRDRNTPEPPQPKDEFVLKFVKP"
     gene            complement(7635..8309)
                     /locus_tag="VIN75_01965"
     CDS             complement(7635..8309)
                     /locus_tag="VIN75_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8972955.1"
                     /translation="MKLLLTSAGVKNASIHRALVGLLGKPVAECHALAVPTAGYGHPM
                     GSPQGAWRFITGHATTPMTELGWKSQGVLELTALPSLPRERWHAWVRAADVLLVNGGD
                     ALYLAHWMRQSGFVDLMPSLQDKVWVGLSAGSMVMAPRVGEDFIEHKPPITGEDRALG
                     VVPFSIFPHVDHPDMPENAMACAARWAATLGNPSYAIDDQTAILVAGGRVEVVSEGTW
                     RWFATA"
     gene            8515..10203
                     /locus_tag="VIN75_01970"
     CDS             8515..10203
                     /locus_tag="VIN75_01970"
                     /inference="COORDINATES: protein motif:HMM:NF023643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N4-asparagine amidase"
                     /protein_id="HEY8972956.1"
                     /translation="MNRPSAVACGCIAMLTASAASAHGPSSGFAEPPVPAVGTRTVAD
                     AELLVPRPRTTPCTVSLFANQEFAGFTPVALTYAPPAACAGPWAKVVLQADYEVTAGR
                     QFDRTAVINLGGVNVYFGTTMEPGSATARAWHVERDVTDVSALLASAQSGEAILGNVV
                     DSTYTGRIFGSASLVFYPRERGEPAADAPQVVAPMAPSLTALSPSNPTLTKTLTLPRN
                     VERLALDVIAQSQASDEFWYTCVPDADASVLQSCGGTAFREVEVAIDGAPAGVAPVFP
                     WIYTGGINPALWAPSPGVQTLDFEPSRIDLTPFAGQLDDGQPHTISLQVSHAQDNFSV
                     TATLLAWRDADAPVLSGAVTQNTLAAPAVSVDDSGLTVDGSNASGRVTVASRRDYRIA
                     GTLFTSHGPVTTVVDQKMKFENTQDFAITDTSYAQKIGQRTDVHTAVTTYAPHGVTLR
                     TTHASYPLSVDFVETLSGNAVVLDTTISQRRRVETRSDGPHGQSWWNTSDETVTPHAT
                     TSYDQTTGAATTTNSASRQRVHVEDSRLGCYDRTIVVTANAVSAVKQGCDDHHR"
     gene            complement(10282..12177)
                     /locus_tag="VIN75_01975"
     CDS             complement(10282..12177)
                     /locus_tag="VIN75_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8972957.1"
                     /translation="MTDSNDLREQLARKNKQLQDIAAQLKTELFGIDAIIDRVIDSVR
                     AWVLLPELVTRPVIVCLWGLTGTGKTQLVRRLAQLLGFYDRFVEVQMDGFSNGAGWRG
                     AQSISGMLAQSGVREGEPGILVLDEFQRFRTIDTKRGEVRVERYQDVWALLSDGRLPP
                     ALSLLGDIESSIAEAAWDAERAEPNETKLRFKLRSWEAQELQRNLKLDEPLMEIMSWT
                     PEQIQERLRAFRENGQQRWETDYSRLLVFVCGNLDEAYEDLATSVDDCDSDADIFHAL
                     SGKLSVIDVKQALNLRFKPEQVARLGNEHVIYPSLSRRAYEQLIEQGCARYAAETGGR
                     CGLHLELDATVREQIYANGVFPAQGTRPLFSSMHAILGTGLAKAALWALERGAAPGET
                     VGLTADGRSLVAHWRGQSQSIAAPFEINRLRQRNNADFRALLAVHEAGHGLVHALLFG
                     RAPQEIKIHVASFEGGYNAYTARKVWSRDNLLDSICTSLAGRAAELLVFGPALSSSGA
                     ESDLRKATETAARMVRHLGHGARIGRIDVSTDSEDNLCTDVEASNAPVEALLQAEHAR
                     ATRLLQAHRESLLALVDELVAHGQVTPQRFAAVSGLPLGRPEEALDPYAQRLAAFRAA
                     APERLSA"
     gene            complement(12170..12412)
                     /locus_tag="VIN75_01980"
     CDS             complement(12170..12412)
                     /locus_tag="VIN75_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972958.1"
                     /translation="MSAQLRSRALQEGRVKALRRMKQPMTLVLETSIGARDPVARALG
                     QRGARGAGRHIRSRGAQRRADRMALQALLRRKDFDD"
     gene            complement(12728..13207)
                     /locus_tag="VIN75_01985"
     CDS             complement(12728..13207)
                     /locus_tag="VIN75_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972959.1"
                     /translation="MHAAHRFTLAALPALLAGLAAAPARADDAADEAFYQRASDCAAA
                     LQFDQLALVARARAGEKNIRPALFEITRLGFAYVGEAYLKGLRDPRGSNMLKAATAAQ
                     KDWPAERHKAMVAECRIEAQKVYDNSGMWKMLVDNKANKRVDRFLSMPPLPASAASR"
     gene            complement(13271..14428)
                     /locus_tag="VIN75_01990"
     CDS             complement(13271..14428)
                     /locus_tag="VIN75_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8972960.1"
                     /translation="MTRRLRYAMVGGGRDAFIGAVHRAAMALDGQYELVAAALSSTPE
                     RSLASGRDLGLPDDRNHGEWQSLLADERSRPAHERADVVVIVTPNHLHFEPARAFVDA
                     GFHVVCDKPLVHTLAQAEELAALVAARGTLFAVTYNYTGYPMVREARELVRSGAIGAV
                     RKVVVEYHQGWLAGALESSDNKQSEWRKDPARGGPAGAMADIGSHAENLVATVTGLEL
                     ESLCADLSAFGPGRQLDDDAGLLLRFAGGARGVMSASQVAAGSENDLRLRVYGERGQL
                     DWRQEEPNLLVHAPGDGPRRTLTRNSPGLGAAARRACRLPPGHPEGFIEAFANLYQGV
                     AADLRLRATQGRPATADEAEYPRIADGVRGVRFIERAVASARSDAKWTPLR"
     gene            complement(14425..15456)
                     /locus_tag="VIN75_01995"
     CDS             complement(14425..15456)
                     /locus_tag="VIN75_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY8972961.1"
                     /translation="MARPITLFTGQWADLPLAELAPLARTMGYDGLELACWGDHFDVQ
                     QALASPAYLRDKRALLAGHGLQCLAIGNHLVGQAVCDLIDARHEAILPARVWGDGEPE
                     GVRQRAARELADTARAAKAFGVGTVTGFTGSSIWHASYAFPPTPQAFWDAGFADFARR
                     FLPILQVFDECDVNFALEVHPTEIAFDIASAERALAAVDQHRRLGFNFDPSHLGYQGV
                     DYIRFIRQFGPRIFNAHMKDVWWGKGDGTVGTFGGHTSFGDPRRGWDFRSVGRGLIDF
                     ESIIVALNDVGYAGPLSVEWEDSRMDRVHGATESAAFCRRLDFEPAKGAFDAVFDKSR
                     QGGMAGSAP"
     gene            complement(15470..>16353)
                     /locus_tag="VIN75_02000"
     CDS             complement(15470..>16353)
                     /locus_tag="VIN75_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8972962.1"
                     /translation="KVTLGVAIPSADHGFTAGIVWWANEAKKELEKKYPDLKVIVKTS
                     KDPGEQANQLQDLVTANRINALVIFPHESAALTKPVQQVHSKGVYVTVVDRGLTDESG
                     QDAYVAGDNTAFGKVPAEYIAKALNGKGDIVALRGIATTLDNERMDAFNSVMKNYPDI
                     HLLDAKYANWNRDDAFKTMQDFLTRFKHIDAVWAADDDMAVGVLKAIDQAHRTDIKLV
                     FGGAGAKGMIKTLKDGTDPRIKANVSYSPKFIYDAIKLTAEARLKGEKLPAKTIIPSV
                     LITRENAKDFYFPDSPF"
BASE COUNT         2262 a         6030 c         5821 g         2240 t
ORIGIN      
        1 tcgcaaggat ccatgatcac gttcaagcgc ccggatgggc aggagctcca gggttatctg
       61 gccaagccgg cgaaggcgga gggcgccccg ggcatcgtcg tcatccagga gtggtggggc
      121 ctgaacgacc agatccgcgg cgtcgccgac cgcctggcgc acgccggcta cctggcgctc
      181 gtgcccgacc tgtaccgcgg caagaccacc gtcgagcgcg aggaggcgca ccacctgatg
      241 acgggcctgg acttcggcga cgccgccagc caggacgtgc gcggcgccgt ccagttcctg
      301 aagacgcgcg cggccaaggt cggctgcgtc ggcttctgca tgggcggcgc gctgacgctg
      361 ctggccgcag ccaacgtgcc cgagctcgac gccgccgtca cctggtacgg ctgcccgccg
      421 ctcgagtacc tcgatgccgg caagatcaag gtgccgctgc agggccactg ggccgaccag
      481 gaccagttct tcccgccgga gcaggtgcgc ggcctgacgg ccaagctgga ggcggccggc
      541 gtgcagcacg acttccacca ctacctcgcg caccacgcgt tcggcaacga gcaggccgtc
      601 ggcgaaggcc gcatcgccga gacgcactac gatccggtgt gggcgcagcg cgcgtgggat
      661 cggacgttcc tgttcttcgc gaagcacctg ggttgacggg ccgcgtcgcg actttcaaga
      721 cctgacaccg caactttcgg cggcgccgcg gcccaccagc gccgcctcgc ggcccgtcga
      781 cacggctgct tgcagcagga gcagatcctc gggtcgcgcc ggccgcgccg aggacaccgc
      841 tgcagcgcga agtcccggca tcggagccac aatcgccatt ccgcccccca ggagcagcgc
      901 catggcccgc ccgcccgtga tcgtcgagac gaatggctgg atcgccacgg tgaccctgga
      961 tcgccccgac tgccgcaacg cggtcgacgg gcccacggcg acgctgctgc gcgaggcctt
     1021 cgagcgcttc gagggcgacg acgagctgcg cgtgctggtg ctgaccggcg ccggtggcca
     1081 gttctgcgcc ggcgccgacc tcaaggcgat ggccgacccg gcgcgccgca acgcggtcga
     1141 gcccaccggc gccgcggccg gcccgatggg cccgacccga ctgcgcatga gcaagccggt
     1201 gatcgccgcg atcgaaggcc atgcggtggc cggcggcctg gagctcgcgc tgatgtgcga
     1261 cctgcgcgtg gccgcgcgca gcgcggtgtt gggcgtgttc tgccgccgct ggggcgtgcc
     1321 gctgatcgac ggcggcaccg tgcgcctgcc gcgcatcgtc ggcgcgggcc gcgcgctcga
     1381 catgatcctg acgggccgcg ccgtgccggc cgacgaggcc ctggcgttcg ggctggtcaa
     1441 ccgcgtcgtg gaagacggcg gcgcactcgc ggcggcgcag gcgctggcgg cggagatcgc
     1501 cgagtttccg caggcctgcc tgcgcgtgga tcgcgcgtcg gcgatggggc aatgggacct
     1561 gtcgctggag gacgcgttgc ggcacgaagg agcggccggc ttcgcggtgg tcgccgccga
     1621 gggcctggcc ggcgcgcagc gcttcgcggc gggcgccgga cgacacggcg cgcccgcggc
     1681 gtagcggcgt ccggcgccgc aggacatgca cccgatgatg cccgcatgat cgacgacgat
     1741 tcgatcggct gcgccagcca cggcacgggc cgcgcgaccg acgtctgcgg tcacctcgcc
     1801 gcggatccgg tgcagcgctg gcacggcaac ccggcctccg tcgacaaccc gtggcccgac
     1861 gcgtgggccg gctgcgcggc ccgtcgctcg aaagctggaa cgacttcgtg gccacgtgcc
     1921 gcgccgacct cggctcgaag caggacgcac tctgactgtg gggtcaggtc ttgaatgttg
     1981 cgcgacgacg catcggctgg atggtgtctc gccgcgcaac attcaagacc tgaccccaca
     2041 gtcgcacagt cgagaccgcg ccgcgcaact ttcaagacct gacaccggtc catgcgcgtg
     2101 atcgcgccac gattcaagcc ctggcgccgg cctcggtgct cgccatctcc atgaaccgca
     2161 gctgcggatc gagcgccagc gcgcgcgtcg cggcgcgggc ctgcgcaggg tccagcggca
     2221 cgagcagctc gatctggccg gccacgctgg tgccgatcgc caggccgtcg cggtacagca
     2281 cgcgcgagcc cgacacgcgc ggcaccttgg ggccggtgag cacggtgccc agcaggttgg
     2341 cggggtccga cgccgacagg cagacgagcg tctcgtccgg cggctggcgg cgcacggcgc
     2401 gcatcgtggc gatggcatcg ggcagcgcga actgctcgcc cgtcacgccc gcgatgaagc
     2461 ggccgcctcg gatctcgccg cgcgcttcca gccggcggca gacgcgcacg agctcgcgcc
     2521 acggcggcag ccaccctgcc tcgcggtcga gcatctcgta gcagacgacg ccatagcggc
     2581 gcagcagcgt gcgcgccacg tgctcgacgg cctcggcgtg cgccgtcgcg tccggcacga
     2641 cctgcggcgc cttgaccacg ttccagcgtc ccgcatcctc gatgccgtac agcgcgacgc
     2701 gccggcgcga gcgcgaagcc gccgtcgccc gcttggccgc gggcaccagc agcgcgcgca
     2761 ggcccgcgta gctgtcgcag ctggcccggc cgcgcaccac gagttcggcc agcgcatcct
     2821 ccagctcggc ccgcagcagg cgcgcgccgt cggccatctc gtcgaagaag gatgcgccgt
     2881 tggcctcgag gaagtccagc acgcgttgcg cccggccgcc cagcgcttcg ccgtcctcgc
     2941 ccgccgcggc ggccgcgatg cgcgaccagg tcggcgccgc gcgccgcggc agcagcagga
     3001 tgggcgtgcc gcgcagcgac gagcgcccgc cgccctgcgt gccgcccgcc agcggccgca
     3061 gccgcgtcca cagcgtgcgg cccgcggtgc acaggtcgtc gagccaggac gatgcgtagt
     3121 ccttcacgcg cgccggcagc acctcggcct cccacagcga cgcgggcgcc tcgaagcctt
     3181 cgagctgcgc cagcacggcc ggcaaggcct cgggcccgct gacgcgcgac gcgcccgcga
     3241 cgtgctgcca ctcgaacagg aagcgcacga agtcgcgcgg ctcgacgggt tcgatctcgc
     3301 ggcgcaggcg cttgagcgtg tagcggtgga tgcgcgcgag cagatggcgc tcgcaccatt
     3361 cgaccgtctc cagccccggg gtgacatggc cctggaacac cgtgccttcc gactgcagcg
     3421 ccgcgagccc ggcctcgatg tcgccgcgcg gcagccgcag ttgctcgacg agcgcgtcga
     3481 cggtgacggg acccaggccg cccagccgcg agcgcagcag ttcgcgcagc gcctcctgca
     3541 gcgtctcgaa cggcgcctca tagccggccg gcggcgtgat cgcgggcgtg gcctcggccg
     3601 ccggccagag cggacgcagc gtcgcgaggc gttcggcggc gacccacagc ccatcctcgc
     3661 ccgcgcgcaa gcgcgtcgcg cgtcccgcgc gcgcgagcgc cgcgagcgcg gcgtcccagt
     3721 gctggttggc ccgcgcctcg tccgcggtga tcgcgcccag cgtcatcaac gcctcgtgca
     3781 cctcgtcggc gctgcgcgcg cgcggccagg cctcctcgcg cacgccggcg atggcgtcgg
     3841 cgtcgagcgc gcccatctcg ccgacattgt ccgcgtcgcc gaatcggcgc gcctgcacgg
     3901 cctgcgtgcg gcgctcctcg atgggcgcgt cgtcgaggta tgcgtagggc cgcgcggtga
     3961 gcgcctcggc ggccagcggc gacggcgcgg gcaggtcgcg cgcgatcacc tcgatggccc
     4021 cggactcgat gccgcgcaac accgcgagcc agccttcggc gtccatcgcc tcgtgcaggc
     4081 agtcgtgcat cgtctgcatc accagcgggt ggtcgggcac ctcgcgcggg ccggcgaggt
     4141 tctcggcgca ggccagctgg tcggggaaca ccgatgcgag caggtcctcg gacttcatgc
     4201 gctgcaattg cggcgccacc ttgcggccgc cgacgaagcg cggcagcgcc agcgccgtcg
     4261 tcgcgttcca gcgccagcgc acggcgaaca gcggcgcatc gagcagcgcc tgcaccagca
     4321 cgtccatggc ggtggccgag cgcaggtagc gcgccacctc gtcgagcggg aagctgtggc
     4381 tggtggacag cgacagcacg atcgcgtcct cggtggccgc ggcctgcagc tcgaagttga
     4441 actggcggca gaagcgcttg cgcagcgcca ggccccaggc gcgattgagc cggctgccga
     4501 atggcgagtg cacgaccagt tgcatgccgc ccgactggtc gaagaagcgc tccatcacga
     4561 tgcgccggtg cgtcggcagt gcgccgagca cggcctgcgc gctcgcgagg tgctcgacga
     4621 tctggcgtgc cgcgtcggcc gcgaggccgc cggccgtcgc atcgaccagt ccctggatcg
     4681 cggccggctg gccttcgcgc cgcacctgct cgcccacggc ctcgcgcagg cgcgccaccg
     4741 ccatcgacag ctcgtcgctg cgccccggcg cctcgccgag ccagaacggg atgttgggcg
     4801 gctggccctt ggcatcctcg acgcgcacgc gcccgggctc gacgcgcagg atgcggtagc
     4861 tcgtgttgcc cagctggaac acgtcgccgg ccaggctctc gacggcgaag tcctcgttga
     4921 cggtgccgat gcggtcggac tgcggctcca gcagcacggt gtagtcaccg gtctcgggga
     4981 tggtcccgcc ggacgtgagc gccgtcatgc gcgcgccgcg gcgctcgcgc agctggtggt
     5041 gcaccgcgtc gcgatgcagg tacgcgccgc gcgggccctg gcgcgtggtg tagccgtcgg
     5101 ccagcatgcg cagcacggcg tcgaagcgct cgcgcggcag cgcggcgtag ggccaggcgc
     5161 ggcgcatcag cgcgtacagc gcgtcctcgt cccagtcgcg gcaggcggtc tcggcgacga
     5221 cctgctgcgc gagcacgtcg agcggcatcg gcggcacgtg cagcgtgtcg agctcgccac
     5281 gctgaacggc ctccagcagc gccgcgcatt cgacgagctc gtcgcgcgac tgcgggaaca
     5341 gccgcgcctt gggcgtgccg ccgacagcgt gccccgcgcg gccggcgcgc tgcaggaaag
     5401 ccgcgatgga acgcggcgag ccgagctggc agacgaggtc gacgtcgccg atgtccagcc
     5461 ccagctccag cgaagccgtg gccaccagca ccttcagctc gccgcgcttg aggcgctgtt
     5521 ccgcatccag ccgcagctcg cgcgcgaggc tgccgtggtg cgcggcgacg gcgtcgtcgc
     5581 ccagcagctc ggccaggtgg cgcgcggcgc gctcggccat gcggcgcgtg ttgacgaaga
     5641 ccagcgtggt gcggtgcgtg gccaccagtt cgacgatgcg ctggtagacc atgtcccact
     5701 gctcgtgcga catcaccgcc gacagcggcg tgggcggcac ctcgagcgcc aggtcgcgct
     5761 gcttcttgta gccgatgtcg acgatggcgc agtcggcctg cccgtcgacg acgccccccg
     5821 cgccgacgag gaagcgcgcg acctcggcga tcggcttctg cgtggccgac agcccgaccc
     5881 gcgtcacgtg ccgcggctcg gcgcgcgccg tcgcggcctt ggggtcgggc aacacgccgt
     5941 cctcgtccgg ctcgtcggga cacggcggaa aattgacgat gttctgcagc cgttccagcg
     6001 acagcgcgag atggctgccg cgcttgttgg cggccagggc gtggatctcg tcgacgatca
     6061 ccgtgcgcac gttggccagc atgcggcggc ccgcgagcga tcccagcagc acgtacagcg
     6121 actcgggcgt cgtcaccagg atgtgcggcg gccggcgcat cgcctgctgg cgctcgcgct
     6181 gcggcgtgtc gccggtgcgc acggccgtgc ggatctccac atcgggcagg ccttgcgaca
     6241 cgagctcggc gcggatgccg gccagcggct cctccaggtt ggcgcggatg tcgttggaca
     6301 gcgccttcag cggcgagacg tagacgacgg ccgtctcgtc cggcagcgcg ccggccacgc
     6361 cgcggcgcac caggtcgtcc agcgcggcga ggaacgccgt cagcgtcttg cccgagccgg
     6421 tgggcgcggc caccaaggtg tcgcgccccg cgcggatgtg cggccacgcc gcgacctgcg
     6481 ccgcggtcgg cgcgccgaag cggcgctcga accagcgcgc gacggcgggg tggaagtctt
     6541 gcaggggcat ggacgaacga aggtggtggc ccggacgtga ttctcaagcg tccgaggctc
     6601 aacccgcgag cctgtccgga tcatgacggg cgacatgcca gcagggccaa gcgcggcacg
     6661 ccgcctgccg tcgccctggc aacatcgccc gctcatcgga agacgacgga gacacgccat
     6721 gcccgcctgt tcgatcgttc gcctcgcacc gcccgcgctg ctggccgcca ccctgctcgg
     6781 ctgtgcctcg acaccgctgc cgcccgagcc gccgcagctg gtgacgacgt cgaccgtgac
     6841 gacgcattcg gtcagctcgt cgacgtcgac cgccgcgccg ttgacgcgcg agcaggtcgc
     6901 cgccatcgtc gccagtcccg accgcaccga cgccgaccgc acgaacgacc agcgccgcca
     6961 tccggtcgac ctcatgacct tcgtcggcgc gcgcccgggc tggacggtgc tcgacgtgag
     7021 caccggcggc ggctacacga ccgagctgct gtcgcgcatg gtcgggccca ccggcatggt
     7081 gtgggcgcag agccggccgc gcaacccgga cgcccccgcg gcgcgccccg cgcagcccga
     7141 gggcggcgcg ccggcgccga cggccgcgcc ggccgccccg tcgcgcggtc cggccgagat
     7201 cctggccggg cgcagcgacc gcatgaaggc cgcgggcgtc gcggccgcgc ccatcgcctt
     7261 cgtcgccctg cccttcgacg acccggtgcc gcccgaggtg gccgatggca agctggacct
     7321 cgtcacgctg atgttcaatt accacgactt cggctggatc cccgtcgacc gcgccagcat
     7381 gaaccgagcc atcttcgcgg cgctcaagcc cggcggcctg tacgtgatcg ctgaccacgc
     7441 ggggcggccc ggcaccggca tcagcgagtc gggcacgctg caccgcatcg aggaatcgtt
     7501 cctgcgccgg gaggtcgagg ccgccggctt ccggctgctg gccgagggcg acttcctgcg
     7561 caacccggac gacccgcgcg accgcaacac gcccgagccg ccgcagccca aggacgagtt
     7621 cgtcctgaag ttcgtcaagc cgtagcgaac cagcgccagg tcccttccga gacgacctcg
     7681 acgcggccgc ccgccaccag gatcgcggtc tggtcgtcga tcgcgtacga cgggttgccg
     7741 agcgtggcgg cccagcgcgc ggcgcaggcc atcgcgttct cgggcatgtc cggatggtcg
     7801 acgtgcggga agatcgagaa cggcaccacg ccgagcgcgc gatcctcgcc ggtgatcggc
     7861 ggcttgtgct cgatgaagtc ctcgccgacg cgcggcgcca tcaccatgct gcccgcgctc
     7921 aggccgaccc agaccttgtc ctgaagcgac ggcatcaggt cgacgaagcc cgactgccgc
     7981 atccagtgcg ccaggtacag cgcgtcgcca ccgttgacga gcaggacgtc cgccgcgcgc
     8041 acccacgcgt gccagcgctc gcgcgggagg ctgggcagcg cggtcagctc gagcacgccc
     8101 tgcgacttcc atcccagctc ggtcatcggc gtcgtggcat ggcccgtgat gaagcgccag
     8161 gctccctgcg gactgcccat cggatggccg tagccggccg tcgggacggc cagggcgtgg
     8221 cactcggcga cgggcttgcc cagcaggccg acgagggccc ggtggatgga ggcgttcttg
     8281 acgccggcgg aggtgaggag cagtttcatg gtgcctttcc cggaggtcct ggcggacagg
     8341 gccagtcccc acacgacgaa cgacgaggcg cgagttcgac atgaccgcgc gacgctcggg
     8401 cgcgacggcg caagcctgca ttcaactgcc gcgcagcgtg cgggtctgcc ccgacgccgg
     8461 ccgcgaccgc gggtgacatc atcgcccgac caacgacaaa ccccagggcc tgccatgaac
     8521 cgtccttccg ccgtcgcgtg cggctgcatc gccatgctca ccgcgtccgc cgccagcgcg
     8581 cacggaccgt cctccggctt cgccgaaccg cccgtgccgg cggtcggcac gcgcacggtg
     8641 gccgacgccg agctgctcgt gccgcggccg cgcaccacac cctgcaccgt atcgctgttc
     8701 gccaaccagg agttcgcggg cttcacgccg gtggcgctga cgtacgcacc gccggccgcc
     8761 tgcgcgggcc cgtgggcgaa ggtggtgctg caggcggact acgaggtcac cgcggggcgg
     8821 cagttcgacc gcacggcggt gatcaacctg ggcggcgtca acgtctactt cggcacgacg
     8881 atggagccgg gttcggccac cgcccgggcg tggcacgtcg agcgcgacgt caccgacgtc
     8941 agcgcgctgc tggcctcggc gcagtccggc gaggcgatcc tgggcaacgt ggtcgactcg
     9001 acctacacgg gccgcatctt cggcagcgcg agcctcgtct tctacccgcg cgaacgcggc
     9061 gagccggcgg ccgacgcgcc gcaggtcgtc gcgccgatgg cgccgtcgct caccgccctg
     9121 tcgccgtcca acccgacgct cacgaagacg ctgaccctgc cgcgcaacgt cgagcgcctc
     9181 gccctggacg tcatcgcgca gagccaggcc tccgacgagt tctggtacac ctgcgtcccc
     9241 gacgccgacg cgagcgtgct gcagagctgc ggcggcaccg cgttccgcga ggtcgaggtc
     9301 gccatcgacg gcgcgcccgc gggcgtggcg ccggtgttcc cgtggatcta caccggcggc
     9361 atcaaccccg cgctgtgggc gccctcgccc ggcgtgcaga cgctcgactt cgagccctcg
     9421 cgcatcgacc tgacgccgtt cgccggccag ctcgacgacg gccagccgca cacgatctcg
     9481 ctgcaggtct cgcacgcgca ggacaacttc tcggtcaccg ccaccctgct cgcatggcgc
     9541 gacgcggacg caccggtgct cagcggcgcc gtgacgcaga acacgctggc cgcgccggcg
     9601 gtctcggtcg acgactcggg cctcaccgtc gacggctcga acgccagcgg ccgcgtgacc
     9661 gtcgcctcca ggcgcgacta ccgcatcgcc ggcacgctgt tcacctcgca tggcccggtc
     9721 acgacggtcg tcgaccagaa gatgaagttc gagaacacgc aggacttcgc catcaccgac
     9781 acgagctatg cccagaagat cgggcagcgc accgacgtgc acaccgccgt caccacctat
     9841 gcgccgcacg gcgtcacgct gcgcaccacg cacgcgagct acccgctgtc ggtcgacttc
     9901 gtcgagacgc tgtccggcaa cgccgtcgtc ctcgacacca ccatctcgca gcggcgccgc
     9961 gtcgagacgc gcagcgacgg cccgcacggc cagtcatggt ggaacacgag cgacgagacc
    10021 gtcacgccgc acgccacgac gtcctacgac cagacgaccg gcgccgcgac gaccacgaac
    10081 tcggccagcc gccagcgcgt gcacgtcgag gacagccggc tcggctgcta cgaccgcacg
    10141 atcgtcgtga cggccaacgc ggtgtcggcg gtcaagcagg gctgcgacga ccatcaccgg
    10201 tgaacgtcgc gcccgcgggc gcgacctgcg ccgcagtcga gcacgcgaaa ccgcattccg
    10261 cgccgcgccg cccgccctgc cctacgcgct gagccgctcc ggcgccgccg cgcgaaacgc
    10321 cgccagccgc tgcgcatacg gatccagcgc ctcctccggc cgccccagcg gcaggcccga
    10381 caccgcggcg aagcgctgcg gcgtgacctg gccgtgcgcg accagttcat ccaccagcgc
    10441 cagcagcgac tcgcgatgcg cctggagaag ccgcgtggcc cgcgcatgct ccgcctgcag
    10501 cagggcctcc accggcgcgt tgctggcctc cacgtccgtg cacaggttgt cctcgctgtc
    10561 ggtcgacacg tcgatgcgcc cgatgcgcgc gccatggccc aggtggcgca ccatgcgggc
    10621 ggccgtctcg gtcgccttgc gcaggtcgct ctcggcgccg ctcgagctca gcgccgggcc
    10681 gaagaccagc agctccgcgg cacggccggc gaggctggtg cagatcgagt ccagcaggtt
    10741 gtcgcgcgac cagaccttgc gcgcggtgta cgcgttgtag ccgccctcga agctggccac
    10801 gtggatcttg atctcctgcg gcgcgcggcc gaacagcagc gcgtggacca gcccgtggcc
    10861 ggcctcgtgc acggccagca gcgcgcggaa atcggcgttg ttgcgctggc gcaggcggtt
    10921 gatctcgaac ggcgcggcga tcgactgcga ctgcccgcgc cagtgcgcga cgaggctgcg
    10981 gccgtcggcc gtcaggccca cggtctcgcc cggcgccgcg ccgcgctcga gcgcccacag
    11041 cgccgccttg gccaggcccg tgcccaggat cgcgtgcatc gacgaaaaca gcggccgcgt
    11101 gccctgcgcc gggaagacgc cgttggcgta gatctgctcg cgcaccgtgg catcgagctc
    11161 gaggtgcagg ccgcagcggc cgcccgtctc ggcggcatag cgcgcgcaac cctgctcgat
    11221 cagctgctcg tacgcgcggc gcgacagcga cggatagatc acgtgctcgt tgcccagccg
    11281 cgcgacctgc tcgggcttga atcgcaggtt gagggcctgc ttgacgtcga tgacgctcag
    11341 cttgccgctc agcgcgtgga agatgtcggc gtcgctgtcg cagtcgtcca cgctggtggc
    11401 caggtcctcg taggcctcgt cgaggttgcc gcagacgaac acgagcagcc ggctgtagtc
    11461 ggtctcccag cgctgctggc cgttctcgcg gaaggcgcgc aggcgctcct ggatctgctc
    11521 gggcgtccag gacatgatct ccatcagcgg ctcgtcgagc ttgaggttgc gctgcagctc
    11581 ctgcgcctcc cagctgcgca gcttgaaccg cagcttggtc tcgttcggct ccgcgcgttc
    11641 ggcgtcccag gcggcctcgg cgatggacga ctcgatgtcg ccgagcagcg agagcgccgg
    11701 cggcaggcgg ccgtcggaca gcagggccca cacgtcctgg tagcgctcga cgcgcacctc
    11761 gccgcgcttg gtgtcgatgg tgcggaagcg ctggaactcg tcgagcacga ggatgcccgg
    11821 ctcgccctcg cgcaccccgg actgcgcgag catgcccgag atgctctgcg cgccgcgcca
    11881 gcccgcgccg ttcgagaagc cgtccatctg cacctccacg aaacggtcgt agaagcccag
    11941 cagctgcgcc aggcggcgca ccagctgcgt cttgcccgtg ccggtcaggc cccacaggca
    12001 gacgatcacc gggcgcgtga cgagctcggg cagcaacacc caggcgcgca ccgagtcgat
    12061 gacgcggtcg atgatcgcgt cgatgccgaa cagctcggtc ttgagctggg cggcgatgtc
    12121 ctgcagctgc ttgttcttgc gcgccagctg ctcgcgcagg tcgttggaat cagtcatcga
    12181 agtcctttcg ccgcagcagc gcctgcaggg ccatgcggtc ggcgcggcgc tgggcgccgc
    12241 gactgcggat atgccggccc gcgccgcgcg cgccgcgctg gcccagcgcg cgcgccacgg
    12301 ggtcgcgcgc gccgatgctc gtctcgagca ccagcgtcat cggctgcttc atgcggcgca
    12361 aggccttgac ccgcccctcc tggagggcgc gggaacggag ttgtgcactc atttcggttc
    12421 cggaaagcaa aagccccggc tcgatggctg cggggctttg cgggagccgg cgcggccgag
    12481 gccgtatctg tggtcagaca cggtccagcc ggggctcggg gaaccgtgtt cagtcgaagg
    12541 tcgttgtgaa cagggaggac aacatggagg gatctccgtc atgcgggcgc tgtcgtgggc
    12601 gccgccagca atggagcgcg aatgatgggg ccggcgacgc cggcacgcaa gctcggcacg
    12661 cagtgcgcgt tgtggcgcgc caacgccagg cgcaccgcgg cacgacgtcg gcgggcgcgt
    12721 gcgaaggcta cctgctcgcc gccgacgccg gcaacggcgg catcgacagg aaacggtcga
    12781 cccgcttgtt ggccttgttg tcgaccagca tcttccacat gcccgagttg tcgtagacct
    12841 tctgcgcctc gatgcggcat tcggcgacca tcgccttgtg gcgctcggcg ggccagtcct
    12901 tctgcgccgc ggtggcggcc ttgagcatgt tgctgccccg cgggtcgcgc aggcctttca
    12961 ggtaggcctc gcccacgtag gcgaagccca gcctggtgat ctcgaacaac gccgggcgga
    13021 tgttcttctc gccggcgcgc gcccgggcga cgagcgcgag ctggtcgaac tgcagcgccg
    13081 ccgcgcagtc ggaggcgcgc tggtagaacg cctcgtccgc ggcgtcgtct gcgcgggccg
    13141 gcgcggcggc caagccggcc agcagggccg gcaacgcggc aagcgtgaat cggtgtgcgg
    13201 cgtgcatcgt cccatcgtac caactgcagg atgagcggac aggcaagcga gattcccgcg
    13261 ccgggtctcc tcagcgcagc ggcgtccact tcgcgtcgct gcgggccgaa gccactgccc
    13321 tctcgatgaa gcgcacgccg cgcacgccgt cggcgattcg cgggtactcg gcctcgtcgg
    13381 cggtggcggg ccgcccctgc gtcgcgcgca gtcgcaggtc ggcggcgacg ccctggtaaa
    13441 gattcgcgaa ggcctcgatg aagccctcgg gatggcccgg cggcagccgg caggcgcggc
    13501 gcgcggcggc gccgaggccg ggcgagttgc gcgtcagcgt gcggcgcggg ccgtcgcccg
    13561 gcgcatgcac cagcaggttc ggttcctcct ggcgccagtc gagctggccc cgctcgccgt
    13621 aaacgcgcag ccgcaagtcg ttctcgctgc ccgcggccac ctgcgacgcc gacatcacgc
    13681 cgcgcgcgcc gcccgcaaag cgcagcagca ggcccgcgtc gtcgtcgagc tggcggccgg
    13741 ggccgaacgc ggacaggtcg gcgcacaggc tttccagctc gaggccggtg acggtggcga
    13801 cgagattctc ggcgtgcgag ccgatgtcgg ccatcgcgcc ggccgggccg ccgcgcgccg
    13861 ggtccttgcg ccactcggac tgcttgttgt ccgacgattc gagcgcgccg gcgagccagc
    13921 cctggtggta ctcgacgacc accttgcgca ccgcgccgat cgcgcccgag cgcacgagct
    13981 cgcgcgcctc gcgcaccatc gggtagccgg tgtagttgta ggtgaccgcg aacagcgtgc
    14041 cgcgcgcggc caccagcgcg gcgagctcct cggcctgcgc cagcgtgtgc accagcggct
    14101 tgtcgcacac gacgtggaaa ccggcgtcga cgaacgcgcg cgccggctcg aagtgcaggt
    14161 ggttgggcgt gacgatgacg acgacgtcgg cgcgctcgtg cgccgggcgc gatcgttcgt
    14221 cggccagcag cgactgccac tcgccgtggt tgcgatcgtc aggcaggccg aggtcgcggc
    14281 ccgaggcgag cgagcgctcg ggcgtcgacg acagcgccgc cgccaccagc tcgtactggc
    14341 cgtccagcgc catcgcggca cggtgcaccg caccgatgaa cgcgtcgcgg ccgccgccca
    14401 ccatcgcgta gcgcaggcgc cgcgtcatgg cgcgctcccc gccatgccgc cctgcctgga
    14461 cttgtcgaag acggcgtcga acgcgccctt ggccggctcg aagtccaggc gccggcagaa
    14521 cgccgcgctc tcggtggcgc catgcacgcg atccatgcgg ctgtcctccc actccaccga
    14581 cagcggcccg gcgtagccga catcgttgag agcgacgatg atcgactcga agtcgatcag
    14641 gccgcggccc acgctgcgga agtcccagcc gcggcgcggg tcgccgaagc tggtgtggcc
    14701 gccgaaggtg cccaccgtgc cgtcgccctt gccccaccac acgtccttca tgtgcgcgtt
    14761 gaagatgcgc gggccgaact ggcggatgaa gcggatgtag tcgacgccct ggtagcccag
    14821 gtggctggga tcgaagttga agcccaagcg gcggtgctgg tccaccgcgg ccaacgcgcg
    14881 ctcggcgctg gcgatgtcga acgcgatctc ggtcgggtgc acctccagcg cgaagttgac
    14941 gtcgcactcg tcgaacacct gcaggatggg caggaagcgc cgcgcgaagt cggcgaagcc
    15001 ggcgtcccag aacgcctgcg gcgtcggcgg gaacgcgtag ctcgcgtgcc agatcgacga
    15061 gccggtgaag ccggtcaccg tcccgacgcc gaacgccttg gcggcgcgcg cggtgtcggc
    15121 cagctctcgc gcggcgcgct ggcgcacgcc ctcgggctcg ccgtcgcccc acacgcgcgc
    15181 cggcaggatc gcctcgtggc gcgcgtcgat caggtcgcac accgcctggc cgaccaggtg
    15241 gttgccgatc gccaggcact gcaggccgtg tccggccagc agcgcgcgct tgtcgcgcag
    15301 gtaggcgggc gacgcgagcg cctgctgcac gtcgaagtgg tcgccccagc acgccagctc
    15361 caggccgtcg tagcccatcg tcctggccag cggcgccagt tcggccaggg gaaggtcggc
    15421 ccactggccg gtgaagagcg tgatggggcg tgccatggcg agcctccggt caaaacgggc
    15481 tgtcggggaa gtagaagtcc ttggcgttct ccctggtgat cagcaccgac gggatgatcg
    15541 tcttggccgg cagcttctcg cccttcaggc gcgcctcggc ggtgagcttg atcgcgtcgt
    15601 agatgaactt gggcgagtag gacacgttgg ccttgatgcg cgggtcggtg ccgtccttga
    15661 gcgtcttgat catgcccttg gcgccggccc cgccgaagac cagcttgatg tccgtccgat
    15721 gcgcctggtc gatcgccttg agcacgccca cggccatgtc gtcgtccgcg gcccacacgg
    15781 cgtcgatgtg cttgaagcgc gtcaggaagt cctgcatcgt cttgaacgcg tcgtcgcggt
    15841 tccagttggc gtacttggcg tccagcaggt ggatgtcggg gtagttcttc atcacgctgt
    15901 tgaacgcgtc catgcgttcg ttgtccagcg tggtggcgat gccgcgcagc gcgacgatgt
    15961 cgcccttgcc gttcagggcc ttggcgatgt attcggccgg caccttgccg aaggcggtgt
    16021 tgtcgccggc gacgtaggcg tcctggccgc tctcgtcggt caggccgcgg tcgaccaccg
    16081 tcacgtacac gcccttggaa tgcacctgct gcacgggctt ggtcagcgcc gccgactcgt
    16141 gcgggaagat caccagcgcg ttgatcctgt tggcggtcac gagatcctgc agctggttgg
    16201 cctgctcgcc cggatccttg gacgtcttga cgatgacctt gaggtcgggg tacttcttct
    16261 cgagttcctt cttggcctcg ttcgcccacc acacgatgcc ggcggtgaag ccgtggtcgg
    16321 cggacgggat cgcgacgccg agcgtgacct tgt
//
LOCUS       DATCRO010000072        13696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_169603, whole genome shotgun sequence.
ACCESSION   DATCRO010000072 DATCRO010000000
VERSION     DATCRO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169603"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..783)
                     /locus_tag="VIN75_02005"
     CDS             complement(244..783)
                     /locus_tag="VIN75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8972963.1"
                     /translation="MSATRSTSENPSRSPCADALEVLSHGHEQTRALADECRRFARHV
                     ECDPAMQQVAEALCRAIHRMAALEEELFFPAARAALEASALVDIAELEHATARQIIRQ
                     LHETDPREPRYEALVLALTECVERHARHEEGELFPRLRESCLDMGTLGQQLNERLSEA
                     DDDSTPMEERRHAVRAATH"
     gene            1003..9858
                     /locus_tag="VIN75_02010"
     CDS             1003..9858
                     /locus_tag="VIN75_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8972964.1"
                     /translation="MTGSDRSFRTLLSHAAEWLHGLAWSRGAGEHDEEEPLRAALFSS
                     DQMVAHGKQLAGRHRLARHHRPDSLLARLASNQRVLENVARQLRQAVADDRPVTPAAE
                     WLLDNMYLIDEEIRTARRHLPHGYSRELPRLADTDPERDGTMPRVYDLALQAIAHADG
                     RLSRGTLSRFVAAYQSGQPLKLGELWAFPIMLRLALIENLRRVAVRVAAALQERDMAE
                     TWASRMLQAAEERPSDLILVIADMARANPSMSSAFVAEFARRLQGQGAALALPLTWME
                     QRLSDENEAIERLVAVEGQTQAASQVSVANSIGSLRLLGATHWPEFVETLSSVEQVLR
                     EDPLGVYGRMDFGTRDTYRHAVEEVAKACGGDEPAVARKAIELAAQGRAAPDAEADGD
                     DRESHVGYWLIGNGRPALEAAMNVRRSGWRRRHGHSPLPAYVGGILAFAMLMSLGPMQ
                     QAAHELDLQLWTPGGIALVLLLMLATSQLALALVNWIVTLSVTPEALPRLDYTLGIEP
                     RSRTLVAVPTLLGKHADVDTLVDALEVRFLANRDPHLQFALLTDLRDARQETLPEDDD
                     LVDYAEARILALNKRYAEPDGNQQRPHPFLLLHRPRRWNPVENTWMGRERKRGKLADL
                     NALLRGRPDADKAFARIVGDTHSLQDVRYVITLDSDTQLPRESASEMVSAMAHPLNRP
                     RSGSGVKKDVVVEGYGILQPRVGLNLPSVGRSGYARMFGGEPGIDPYTRAVSDVYQDL
                     MGEGSFVGKGIYDVDAFERATHGSMPENRILSHDLIEGCYARSGLLSDVQLLEDTPSR
                     YSVDMARRHRWVRGDWQLIGWLRRRLHALPGAPRNPLSALSKWKLFDNLRRSLVPAAL
                     IALAVLGWARLPEPFMWTLRIVVIVGIVPFAAHVMGLARAPLNWFTASPSPRRLMPIA
                     LQLMQLGHTLACLPTEAVATLDAIGRTIWRMVRGRRLLEWTASADLRAGHPPGTPAAL
                     RDAVRALWAGPTAAVLITALLAYVRAWALPAAAPLLLLWFCSPLIVWWADRPLALPRS
                     ELSAAQRRFLRRVARRTWAFFETFVGAEDNHLPPDNVQLHPVARVAHRTSPTNIGFSL
                     LANLTARDCGFITLDQTLTRIAATITTLERLERHRGHFYNWYDTQSLLPLAPRYISTV
                     DSGNLVAQFMTLRMALLALPDEPPFSPLWCMGIADVLALIRESLPTNTHLPALDRAEK
                     AVQAACADMPASAAGLAIVLESFETMSRDVLAAVEGALATHAARKDAADEEDTGEALR
                     WARSLVAQCEAGRDEMAAFAGLAMPLSADASPAAVAAAARAPLPTLGVLASLGIGEAA
                     VYLDRIQELARRVDALSEQEQAFMYDDRRHLMSIGYNVDERRLDAGHYDLLASEARLG
                     IFTAIARGQLPQESWFALGRLLTAHDGNPVLMSWSGSMFEYLMPPLIMPNFEQTLLDQ
                     TCRAAVRRQIDYGRERGVPWGISESGYNATDTALNYQYRAFGVPGLGLKRGLGDELVI
                     APYATAMATIVEPVAACANLQRLAALGAAGEFGFYEAVDYTPMRVPRGQTKAVVRSFM
                     AHHQGMSLLAIEQALGHSRMQQHFASDRSVQATLMLLHERVPKDVMPIVAEATMEPAA
                     PRAVSASTETSMRVFTDPLTPTPEVQLLSNGNYHMMVTQAGGGYSRYKDMAVTRWRED
                     MTRDDWGSFAYLRDVDSGEYWSTAHQPTGRKATDYQAIFTEGRAEFRRRDAGIDTHTE
                     IAVSPEDAIELRRLRIKNTTRRVRTIEVTSYCEVVLQTPAADVQHPAFGKLFVQTEVV
                     EGLPALLANRRPRAERDPSPWMFHLMAVHAPRGVDNVSAVSHETDRARFIGRGGTLRA
                     PAAMFGGDALSNSQGSVLDPVAASRCAITLQPDQTVIVDLVYGVGDKRADCLALIEKY
                     RDRRLAERVFELAWTHAQVLLRQLNASEADAQMYARLAGAVLFSQGALRADPSIIKQN
                     RRGQSGLWGYAISGDLPIVLVQVSDVANLELVRQLVMAHAWWRLKGLAVDLVIWNEER
                     DIYRQRLNEQIMGLIAAGVEAHVVDRPGGIFVRHADQIPHEDRILLLSVARAVLSDRQ
                     GTLSEQLNRRLRTERRTVPRSVGRTAGPERRLAPFVPTRNLRPDAAASPRIPTGDLAL
                     FNGYGGYGKEGREYVVAPPTGVRTPAPWANVIANPDFGTVISESGAAYTWAENAHEWR
                     LTPWHNDPVSDPSSETFHLRDEETGAAWMPHGVALNPDGTPGRAAVARHGFGYSIFEQ
                     HAQGIRSELRVFCAIDAPIKFSMLTIRNDSGRPRRISATGYVEWVLGALRPATAPHIV
                     TDHQDDGPATARNPYSGDHAEYMGFFDVDAEHQLAGSMTCDRTEFVGRNGNLAEPMAL
                     RRAGLSGRFGAAMDPCAAFQVPLDLAEGESRTVVFRLGMGKNAAEATRTALKYRGDAV
                     AEAEFERAVGHWSRTLGAVKVRTPDAALDALANGWLVYQTISCRLWARSGYYQSGGAF
                     GFRDQLQDAMALVHTRPELLRAQLLLAASRQFEEGDVQHWWHPPQGRGVRTHISDDYL
                     WLPLALCRYIEATGDVAVMSVPVSFLEGRPVPPDAESYFDLPNVSHRTAPLYEHAALA
                     VRHGLRLGEHGLPLIGAGDWNDGMNLIGEHGKGESVWMAFFLCEVLREFIKLARLRGD
                     DAFAQTCAEARERLAAAVEANAWDGDWYRRAYFDDGTPLGTATGVECQIDSIAQSWSV
                     LSGIAPRERARKALDSLAARLVDPQQRIVKLLDPPFNGKGPNPGYISGYVPGVRENGG
                     QYTHGAIWAAMAFAASGDARRSWDVVDMINPVNHSRTPAEVAVYKVEPYVMTADVYSV
                     APHTGRGGWSWYTGSAGWMYRLLVEAVLGLRLEMVDGQPTLLVDPRIPPHWSSFEVDY
                     TYKSAFYRLQFDRTGHDRQPQVTLDGRALEGNRLPLADDARQHSVQVALPSMMPVPEG
                     ESSELLL"
     gene            9909..10310
                     /locus_tag="VIN75_02015"
     CDS             9909..10310
                     /locus_tag="VIN75_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8972965.1"
                     /translation="MLHHLSFGVADLRRSAAFYDAALGTLGFVRVWGDFEGGANDQAV
                     GYGREGGGDKFAIKQRAALPIVPPPGLHIAFAAPDHAAIHRFHAAALAHGGTDNGGPG
                     PRPHYGPHYYAAFVIDPDGYHVEAVLNSPAP"
     gene            10525..12516
                     /locus_tag="VIN75_02020"
     CDS             10525..12516
                     /locus_tag="VIN75_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8972966.1"
                     /translation="MRILRATLGRTGLLAAIMLAAGARAAEAPPGPEKFFQHPRMVAA
                     SLSPDGQTVALLMAANATGRVRLIALDVKTAKFTSLAGFEDADIADFHWVNAHRLVFE
                     LGDRDAPVGDIDTASGLYGVNTDGSALRELVSRHGQPFFERGGDHSRVLPWNTWFLQA
                     VDGVSEDVYVAHAQEIDEKHADYLQLQRLDTLHGHVADVPTPPHVVEWIFDPKGELRV
                     VVTDKANHSKVMTREAGGQEWATLGDFEHYVDDKSFDPSYIDQDGNLYVEARNGGDKV
                     AVWTYDLAHRKMADKAWLLSQKYDLEPSYVTVGGKLAGVRYAADADVTQWVVPELEAL
                     QAKVDKLLPSTTNRLSVAANGNLDTVVIRATSDRVPGLFFILDQTRGKLLKLGSAMPD
                     IEPGTQATLDLVQYKARDGLEIPAWLTIPAGAERKNLPLVVLVHGGPYVRGRTWDWDP
                     ETQFLAARGYAVLEPEYRGSTGYGQKLFQSGWKQWGLAMQDDIADGVKWAVAQGIVDP
                     KRVCIAGASYGGYAVLMGLARDPDLYRCGIEWVGVTDMNLMYTADWSDMSDAWKQYGM
                     PALVGDPVADAAQLKATSPTQLVAKIHAPVLMAYGGKDKRVPIEHGERFRDALKKQPG
                     AKVEWVVYDKEGHGWRSFETRVDFWNRAARFLDASIGAR"
     gene            complement(12544..12726)
                     /locus_tag="VIN75_02025"
     CDS             complement(12544..12726)
                     /locus_tag="VIN75_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY8972967.1"
                     /translation="MNKDQVQGRIAQVKGDLKKLAGRATGNRRLQGDGIIDRARGKAQ
                     AGYGDARERLRKAFPR"
     gene            complement(12893..13414)
                     /locus_tag="VIN75_02030"
     CDS             complement(12893..13414)
                     /locus_tag="VIN75_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972968.1"
                     /translation="MPDPRLVTGLFPDRDSAERAYSSVAARGYDRDDVNLVMSDETRA
                     RHFPADAAVATELGTKAAEGAGIGGAIGGTVGAITAAVAAMGTSLVLPGLGLVIAGPI
                     AAALAGAGAGGAAGGLVGALIGWGIPEERIAEYESGIREGAILMGVRPRNAEDALAFE
                     HSWRDANGQLIRS"
BASE COUNT         1964 a         5069 c         4812 g         1851 t
ORIGIN      
        1 tcgtggtgga ctgcgcggtc gcgaagccga cggtcgccag cagggccgag gcggccaggg
       61 cggtgatgga cttgtgcatc ggggacatgt gtgaactcct gtgcgttggg acgcgcgcgg
      121 cgtccggtac ggatgccggt gaccgtatcc acggcctccg gctggcaggg ttcgaaaggc
      181 aacgcgtgtg cctatcgcgg gccgtctcgt aggacggtcc gccatgtccg cgtaggctcc
      241 gcgtcagtgc gtcgcggccc gcacggcgtg gcgccgttcc tccatgggcg tgctgtcgtc
      301 gtcggcctcc gacaggcgct cgttcaactg ctggccgagc gtgcccatgt ccaggcacga
      361 ctcgcgcagc cgcgggaaca gctcgccctc ctcgtggcgc gcgtggcgct ccacgcactc
      421 cgtcagcgcc aacaccagcg cctcgtagcg cggctcgcgc ggatcggtct cgtgcaactg
      481 gcggatgatc tggcgcgcgg tggcgtgctc cagttcggcg atgtccacca acgccgacgc
      541 ctcgagcgcg gcgcgcgccg ccgggaagaa cagctcctcc tcgagcgccg ccatccggtg
      601 gatcgcgcgg cacagcgcct cggccacctg ctgcatcgcg ggatcgcatt cgacgtggcg
      661 cgcgaagcgc cggcattcgt cggccagcgc ccgggtctgt tcatggccgt gcgacagcac
      721 ctcgagcgcg tcggcgcacg ggctgcggct ggggttctcg gaggtggatc gggtggcgga
      781 catggctggc accgggttgc gacaagggga gatcggcgca cgaggcagcg accttggatg
      841 gccctgatcc ggcaagggat gtgccatccc gcttgcagca acgtttacgc cggtcgcatg
      901 ctggtcgtca agccccggcc gcgcatgctg cgcaacggac gggctcaccg cttgccgcag
      961 gcgcggccat tggtccttcg cggaccccag ggaaaacaat caatgacggg atccgaccgc
     1021 tccttccgca ccctgctctc gcacgccgcg gagtggctcc atgggctcgc gtggagccgt
     1081 ggcgccggcg agcacgacga ggaggagccg ctgcgggccg cgctgttcag cagcgaccag
     1141 atggtggcgc acggcaagca gctggccggc cgtcatcgcc tggcccggca ccaccgcccc
     1201 gacagcctgc tggcgcggct cgcgtccaac cagcgcgtgc tggagaacgt cgcgcgccag
     1261 ttgcgccagg ccgtcgccga cgaccgcccg gtcacgccgg ccgccgagtg gctgctggac
     1321 aacatgtacc tcatcgacga ggagatccgc accgccaggc gccatctccc gcacggctac
     1381 agccgcgagc tgccgcgcct ggccgacacc gatcccgagc gcgacggcac catgccgcgc
     1441 gtctacgacc tcgccctgca ggccatcgcg cacgccgacg gccgcctgag ccgcggcacg
     1501 ctgagccgct tcgtcgccgc gtaccagagc ggccagccgc tgaagctggg cgagctgtgg
     1561 gccttcccga tcatgctgcg gctggcgctc atcgagaacc tgcgccgggt ggcggtgcgc
     1621 gtggcggcgg ccctgcagga acgcgacatg gccgagacct gggcgtcgcg catgctgcag
     1681 gccgccgagg agcgtcccag cgacctgatc ctggtcatcg ccgacatggc ccgcgccaac
     1741 ccgagcatga gcagcgcgtt cgtcgcggag ttcgcgcgcc gcttgcaggg ccagggcgcc
     1801 gcgctggcgc tgcccctcac gtggatggag cagcgcctgt ccgacgagaa cgaggcgatc
     1861 gaacgcctgg tggccgtcga ggggcagacg caggccgcca gccaggtgtc ggtggccaac
     1921 agcatcggca gcctgcgcct gctgggcgcc acgcactggc ccgagttcgt cgagacgctc
     1981 agcagcgtcg agcaggtgct gcgcgaggat ccgctgggcg tctacgggcg catggacttc
     2041 ggcacccgcg acacctaccg ccacgccgtc gaggaggtgg ccaaggcctg cggcggcgac
     2101 gagccggccg tcgcgcgcaa ggcgatcgag ctggccgccc agggccgcgc cgcgccggac
     2161 gccgaggccg acggcgacga ccgcgagtcg cacgtgggct actggctgat cggcaacggc
     2221 cgcccggcgc tcgaggcggc gatgaacgtg cgccgcagcg gctggcggcg ccgccacggc
     2281 cattcgccgc tgccggccta cgtcggcggc atcctcgcgt tcgcgatgct gatgtcgctc
     2341 ggcccgatgc agcaggccgc gcacgagctc gacctgcagc tgtggacgcc cggcggcatc
     2401 gcgctggtgc tgctgctgat gctggccacc agccagctcg cgctggcgct ggtcaactgg
     2461 atcgtcacgc tcagcgtcac gcccgaggcg ctgccgcggc tggactacac gctgggcatc
     2521 gagccgcgct cgcggacgct ggtggcggtg cccaccctgc tgggcaagca cgcggacgtc
     2581 gacaccctcg tcgacgcgct cgaggtgcgt ttcctcgcca accgcgaccc gcacctgcag
     2641 ttcgcgctgc tgaccgacct gcgcgacgcc aggcaggaga cgctgcccga ggacgacgac
     2701 ctcgtcgact acgccgaggc ccgcatcctc gcgctcaaca agcgctacgc cgagccggac
     2761 ggcaaccagc agcgcccgca cccgttcctg ctgctgcacc ggccgcgccg ctggaacccg
     2821 gtcgagaaca cctggatggg ccgcgagcgc aagcgcggca agctggccga cctgaacgcg
     2881 ctgctgcgcg gccggcccga cgcggacaag gcgttcgcgc gcatcgtcgg cgacacccac
     2941 tcgctgcagg acgtgcgcta cgtcatcacg ctggactccg acacgcagct gccgcgcgag
     3001 tcggccagcg agatggtctc ggcgatggcg cacccgctga accggccgcg ctctggcagc
     3061 ggcgtcaaga aggacgtggt cgtcgagggc tacggcatcc tgcagccgcg cgtcggcctg
     3121 aacctgccga gcgtcggccg ctcgggctat gcgcgcatgt tcggcggcga gccggggatc
     3181 gatccgtaca cccgcgccgt gtcggacgtc taccaggacc tgatgggcga aggctcgttc
     3241 gtcggcaagg gcatctacga cgtcgatgcg ttcgagcgcg cgacgcacgg cagcatgccg
     3301 gagaaccgca tcctcagcca cgacctgatc gagggctgct acgcgcgctc cggtctgctg
     3361 tcggacgtgc agctgctgga agacacgccg tcccgctaca gcgtcgacat ggcccgccgc
     3421 caccgctggg tgcggggcga ctggcagctg atcggctggc tgcgccggcg cctgcacgcg
     3481 ctgcccggcg cgccgcgcaa cccgctgtcg gcgctgtcca agtggaagct gttcgacaac
     3541 ctgcgccgca gcctggtgcc cgcggcgctg atcgcgctgg ccgtgctggg ctgggcgcgg
     3601 ctgcccgagc cgttcatgtg gacgctgcgc atcgtcgtga tcgtgggcat cgtgccgttc
     3661 gcggcgcacg tcatgggcct cgcccgggcg ccgctcaact ggttcacggc ctcgccctcg
     3721 ccgcgccgcc tgatgcccat cgcgctgcag ctgatgcagc tcgggcacac gctggcctgc
     3781 ctgcccactg aggccgtcgc cacgctggac gccatcggcc gcacgatctg gcgcatggtg
     3841 cgcggccgcc gcctgctgga atggacggcc tcggccgact tgcgcgccgg ccacccgccc
     3901 ggcacgcccg cggcgttgcg cgacgccgtg cgcgcgctgt gggccggtcc gaccgccgcc
     3961 gtgctgatca ccgccctgct cgcctacgtg cgtgcctggg cgctgcccgc ggccgccccg
     4021 ctgctgctgc tgtggttctg ctcgccgctg atcgtctggt gggccgaccg tccgctggcc
     4081 ttgccgcgca gcgagctcag cgcggcgcag cgtcgcttcc tgcgccgcgt cgcgcgccgc
     4141 acctgggcgt tcttcgagac cttcgtcggc gccgaggaca accaccttcc gcccgacaac
     4201 gtgcagctgc atccggtggc gcgcgtcgcg caccggacct cgccgaccaa catcggcttc
     4261 tcgctgctgg ccaacctgac ggcgcgcgac tgcggcttca tcacgctgga ccagacgctc
     4321 acgcgcatcg ccgccacgat caccacgctc gagcgcctgg agcgccaccg cgggcacttc
     4381 tacaactggt acgacacgca gtcgctgctg ccgctggcgc cgcgctacat ctccacggtg
     4441 gacagcggca acctcgtcgc gcagttcatg acgctgcgca tggcgctgct ggcgctgccc
     4501 gacgagccgc cgttctcgcc gctgtggtgc atgggcatcg ccgacgtgct cgcgctgatc
     4561 cgcgagagcc tgcccaccaa cacccacctg cccgcgctgg accgcgccga gaaggcggtg
     4621 caagccgcct gcgccgacat gcccgcctcc gcggccggcc tggccatcgt gctcgagagc
     4681 ttcgagacga tgtcgcgcga cgtgctcgcg gccgtcgagg gcgcgctcgc cacccacgcc
     4741 gctcgcaagg acgccgccga cgaggaggac accggcgagg cgctgcgctg ggcgcgctcg
     4801 ctggtcgcgc agtgcgaggc cggacgcgac gagatggccg cgttcgcggg actggcgatg
     4861 ccgctgtcgg cggacgcgtc gcccgccgcc gtcgccgccg ctgcgcgcgc tcccctgccc
     4921 acgctgggcg tgctggcgtc gctgggcatc ggcgaggccg cggtctacct cgaccgcatc
     4981 caggagctgg cacgccgcgt cgacgcgctc agcgagcagg aacaggcctt catgtacgac
     5041 gaccgccggc acctgatgtc gatcggctac aacgtggacg aacggcgcct ggacgccggg
     5101 cactacgacc tgctggcctc ggaggcgcgg ctgggcatct tcaccgcgat cgcgcgcggg
     5161 cagctgcccc aggaaagctg gttcgcgctc ggccgcctgc tcacggcgca cgacggcaac
     5221 ccggtgctga tgtcgtggag cggctcgatg ttcgagtacc tgatgccgcc gctgatcatg
     5281 cccaacttcg agcagacgct gctcgaccag acttgccgcg ccgcggtgcg ccgccagatc
     5341 gactacggcc gcgagcgcgg cgtgccctgg ggcatctcgg agtcgggcta caacgccacc
     5401 gacacggcgc tgaactacca gtaccgcgcg ttcggcgtgc cagggctggg cctgaagcgc
     5461 ggcctcggcg acgagctggt catcgcgccc tatgccaccg cgatggccac gatcgtcgag
     5521 ccggtggccg cctgcgccaa cctgcagcgc ctggccgccc tcggcgcggc cggcgagttc
     5581 ggcttctacg aggccgtcga ctacacgccg atgcgcgtgc cgcgcggcca gaccaaggcc
     5641 gtcgtgcgct cgttcatggc ccaccaccag ggcatgagcc tgctggccat cgagcaggcg
     5701 ctgggccatt cgcgcatgca gcagcacttc gcgtccgacc gcagcgtgca ggcgaccctg
     5761 atgctgctgc acgagcgcgt gcccaaggac gtgatgccca tcgtcgcgga ggccacgatg
     5821 gagccggcgg cgccgcgcgc cgtgtcggcc agcaccgaga cgagcatgcg cgtcttcacc
     5881 gacccgttga cgcccacgcc cgaggtgcag ctgctgtcca acggcaacta ccacatgatg
     5941 gtcacgcagg ccggcggcgg ctacagccgc tacaaggaca tggccgtcac gcgctggcgc
     6001 gaggacatga cgcgcgacga ctggggcagc ttcgcgtacc tgcgcgacgt cgactcgggc
     6061 gagtactggt cgacggcgca ccagcccacg ggccgcaagg cgaccgacta ccaggccatc
     6121 ttcaccgagg gacgcgcgga gttccgccgg cgcgacgcgg gtatcgacac ccacaccgag
     6181 atcgccgtct cgcccgagga cgccatcgag ctgcggcgcc tgcgcatcaa gaacacgacg
     6241 cggcgggtgc gcacgatcga ggtcaccagc tactgcgagg tcgtgctgca gacgccggcg
     6301 gccgacgtgc agcacccggc cttcggcaag ctgttcgtgc agaccgaggt cgtcgagggc
     6361 ctgcccgccc tgctcgccaa ccggcggccg cgcgcggaga gggatccgtc gccgtggatg
     6421 ttccacctga tggcggtgca cgcgccgcgc ggcgtggaca acgtctcggc cgtgtcgcac
     6481 gagacggatc gcgcccgctt catcggccgc ggcggcacgc tgcgcgcgcc ggcggcgatg
     6541 ttcggcggcg acgcgctgtc gaactcgcag ggctcggtgc tcgacccggt ggccgccagc
     6601 cgctgcgcca tcacgctgca gcccgaccag accgtcatcg tcgacctcgt ctacggcgtc
     6661 ggcgacaagc gcgccgactg cctggcgttg atcgagaagt accgcgaccg ccgcctggcc
     6721 gagcgcgtgt tcgagctcgc ctggacgcac gcgcaggtgc tgctgcgcca actcaacgcc
     6781 agcgaggccg acgcgcagat gtacgcgcgc ctggccggcg ccgtgctgtt cagccagggc
     6841 gcgctgcgcg ccgatccgtc catcatcaag cagaaccggc gcggccagtc cgggttgtgg
     6901 ggctacgcga tctcgggcga cctgcccatc gtgctggtgc aggtctccga cgtcgccaac
     6961 ctcgagctcg tgcgccagct ggtgatggcc cacgcgtggt ggcggctcaa gggcctggcc
     7021 gtcgacctcg tcatctggaa cgaggagcgc gacatctatc gccagcgcct gaacgagcag
     7081 atcatggggc tgatcgcggc gggcgtggag gcgcacgtcg tcgaccgccc gggcggcatc
     7141 ttcgtgcgcc atgccgacca gatcccgcac gaggatcgca tcctgctgct gtcggtggcg
     7201 cgcgcggtgc tgagcgaccg ccagggcacg ctctccgagc agctgaaccg gcggctgcgc
     7261 accgagcgcc gcaccgtgcc gcgcagcgtg gggcgcaccg ccggtccgga gcgccgcctc
     7321 gccccgttcg tgcccacgcg caacctgcgg cccgacgcgg ccgcgtcgcc gcgcatcccc
     7381 accggcgacc tggcgctgtt caacggctac ggcggctacg gcaaggaagg ccgcgagtac
     7441 gtcgtcgcgc cgcccacggg cgtgcgcacg ccggcgccgt gggccaacgt catcgccaac
     7501 ccggacttcg gcaccgtcat ctcggagtcc ggcgcggcct acacctgggc cgagaacgcg
     7561 cacgaatggc gcctgacgcc gtggcacaac gaccccgtct ccgatccgag cagcgagacg
     7621 ttccacctgc gcgacgagga gaccggcgcg gcgtggatgc cgcacggcgt cgcgctcaac
     7681 cccgacggca cgcccggccg cgccgccgtc gcgcgccacg gcttcggcta cagcatcttc
     7741 gagcagcacg cgcaaggcat ccgcagcgag ctgcgcgtgt tctgcgcgat cgatgcaccg
     7801 atcaagttct cgatgctgac gatccgcaac gactcgggac ggccgcgccg catcagcgcc
     7861 accggctacg tcgaatgggt gctgggcgcc ctgcggccgg ccaccgcgcc gcacatcgtc
     7921 accgaccacc aggacgacgg ccccgcgacc gcgcgcaacc cgtacagcgg cgaccacgcc
     7981 gaatacatgg gcttcttcga cgtcgacgcc gagcaccagc tcgcgggcag catgacctgc
     8041 gaccgcaccg agttcgtcgg ccgcaacggc aacctggccg agccgatggc gctgcggcgc
     8101 gcgggcctgt cgggccgctt cggcgcggcg atggatccgt gcgccgcgtt ccaggtgccg
     8161 ctggacctgg ccgagggcga gtcgcgcacc gtcgtgttcc ggctcggcat gggcaagaac
     8221 gccgccgaag ccacgcgcac cgccctgaag taccgcggcg acgccgtggc cgaggccgag
     8281 ttcgagcgcg ccgtcggcca ctggtcgagg acgctgggcg ccgtgaaggt gcgcacgccc
     8341 gacgcggcgc tcgacgcgct ggccaacggc tggctcgtct accagaccat ctcgtgccgg
     8401 ctgtgggccc gcagcggcta ctaccagtcg ggcggcgcct tcggtttccg cgaccagttg
     8461 caggacgcga tggcgctggt gcacacccgg cccgagctgc tgcgcgcgca gctgctgctg
     8521 gccgccagcc gccagttcga ggaaggcgac gtgcagcact ggtggcaccc gccgcagggc
     8581 cgcggcgtgc ggacgcacat cagcgacgac tacctgtggc tgccgctggc gctgtgccgc
     8641 tacatcgagg ccacgggaga cgtggcggtg atgtcggtgc ccgtgagctt cctcgagggg
     8701 cggccggtgc cgccggatgc ggagtcctac ttcgacctgc ccaacgtctc gcaccggacg
     8761 gcgccgctgt acgagcacgc ggcgctggcc gtcaggcatg ggctgcgcct gggcgagcac
     8821 ggcctgccgc tgatcggcgc aggcgactgg aacgacggca tgaacctgat cggcgagcac
     8881 ggcaagggcg agagcgtctg gatggcgttc ttcctgtgcg aggtgctgcg cgagttcatc
     8941 aagctggcgc gcctgcgcgg cgacgacgcg ttcgcgcaga cctgcgcgga ggcgcgcgag
     9001 aggctggccg ccgccgtcga ggccaacgcc tgggacggcg actggtaccg ccgggcgtat
     9061 ttcgacgatg gcacgccgct gggcaccgcc accggcgtcg aatgccagat cgactcgatc
     9121 gcgcagagct ggtcggtgct ctcgggcatt gcgccgcgcg agcgcgcgcg caaggcgctc
     9181 gactcgctgg ccgcgcgcct ggtggacccg cagcagcgca tcgtcaagct gctcgacccg
     9241 ccgttcaacg gcaagggccc gaatcccggc tacatcagcg gctacgtgcc gggcgtgcgc
     9301 gagaacggcg ggcagtacac gcatggcgcg atctgggcgg cgatggcgtt cgcggccagc
     9361 ggcgacgcgc ggcgctcgtg ggacgtggtc gacatgatca accccgtgaa ccattcgcgc
     9421 acgccggcag aggtggccgt ctacaaggtc gagccctacg tgatgacggc cgacgtctac
     9481 agcgtcgcgc cgcacaccgg ccgcggcggc tggagctggt acacgggctc ggccggctgg
     9541 atgtaccggc tgctggtcga ggcggtgctg ggcctgcggc tggagatggt ggacggccag
     9601 cccacgctgc tggtcgaccc gcgcatcccg ccgcactgga gcagcttcga ggtcgactac
     9661 acgtacaagt cggcgttcta ccggctgcag ttcgaccgca ccggccacga ccggcagccg
     9721 caggtcacgc tcgacgggcg cgcgctcgaa ggcaaccgcc tgcctctggc cgacgacgcg
     9781 cgccagcaca gcgtgcaggt ggcgctgccg tcgatgatgc cggtgcccga gggcgagtcg
     9841 agcgagctgc tgctgtaggc ggcgccctgc ggtcagaatc gggcatcccc gttccggaaa
     9901 cgaccgccat gctgcatcac ctgtcgttcg gcgtcgccga cctgcgccgc tccgccgcct
     9961 tctacgacgc cgcgctcggc accctcggct tcgtccgcgt ctggggcgac ttcgagggcg
    10021 gagcgaacga ccaggccgtc ggctacggcc gcgaaggcgg cggcgacaag ttcgcgatca
    10081 agcagcgcgc ggccttgccc atcgtcccgc cgcccggctt gcacatcgcc ttcgccgccc
    10141 ccgaccacgc ggcgatccac cgcttccatg cggccgccct cgcccacggc ggcacggaca
    10201 acggcggccc gggcccgcgc ccgcactacg gcccgcacta ctacgccgcc ttcgtgatcg
    10261 accccgatgg ctaccacgtc gaggccgtcc tgaactcgcc ggcgccctga ccggcccgcg
    10321 cggccgcccg cgcccagggc tgagcccgat gcggggcgcg gccgcgcgcc ggtagactcc
    10381 cctcttttcg cagatccacg acagctgccg gcgcccgcgc gccaggctgc ggagacttct
    10441 ggaaccgtgt cgcgacgttc ctggaagcca acatcggcgc gcactgagcg ccggacgaca
    10501 acgacgactc gacagggagc acacatgcgc attcttcgcg cgacgctggg ccgcacaggc
    10561 ctgctggccg ccatcatgct ggccgcgggc gcccgggcgg ccgaagcccc gcccgggccg
    10621 gagaagttct tccagcaccc gcgcatggtc gcggcctcgc tgtcccccga cggccagacg
    10681 gtcgcgctgc tgatggccgc caacgccacc ggccgcgtgc gcctcatcgc gctcgacgtg
    10741 aagaccgcga agttcacgtc cctggccggc ttcgaggacg cggacatcgc cgacttccac
    10801 tgggtcaacg cccatcgcct ggtcttcgaa ctgggcgacc gcgacgcgcc cgtcggcgac
    10861 atcgacaccg cctcgggcct gtacggcgtc aacaccgacg gcagcgccct gcgcgagctc
    10921 gtgagccgcc acggccagcc cttcttcgag cgcggcggcg accactcgcg cgtgctgccc
    10981 tggaacacct ggttcctgca ggcggtcgac ggcgtctccg aggacgtcta cgtcgcgcac
    11041 gcgcaggaga tcgacgaaaa gcacgccgac tacctgcagc tgcagcggct ggacacgctg
    11101 catggccacg tcgccgacgt gcccacgccg ccgcacgtcg tcgaatggat cttcgatccc
    11161 aagggcgagc tgcgcgtggt cgtgaccgac aaggccaacc acagcaaggt gatgacgcgc
    11221 gaggccggcg gccaggaatg ggcgaccctc ggcgacttcg agcactacgt cgacgacaag
    11281 agcttcgacc ccagctacat cgaccaggac ggcaacctgt acgtcgaggc gcgcaacggc
    11341 ggcgacaagg tggccgtgtg gacgtacgac ctcgcccacc gcaagatggc cgacaaggcc
    11401 tggctcctgt cgcagaagta cgacctcgag ccgtcgtacg tcaccgtggg cggcaagctt
    11461 gccggcgtgc gctatgcggc cgacgcggac gtcacgcagt gggtggtgcc cgagctcgag
    11521 gcgctgcagg ccaaggtcga caagctgctg ccctcgacga ccaaccgcct gagcgtcgcg
    11581 gccaacggca acctcgacac ggtcgtgatc cgcgccacgt ccgaccgcgt gcccgggctg
    11641 ttcttcatcc tcgaccaaac ccggggcaag ctgctcaagc tgggctccgc gatgcccgac
    11701 atcgagcccg ggacgcaggc gacgctcgac ctcgtgcagt acaaggcgcg cgacgggctg
    11761 gagatcccgg cctggctcac catcccggcc ggcgccgagc gcaagaacct gccgctggtg
    11821 gtgctggtgc acggcggccc ctatgtgcgg ggccgcacgt gggactggga tcccgagacg
    11881 cagttcctcg cggcgcgcgg ctatgccgtc ctcgagccgg agtaccgcgg cagcacgggc
    11941 tacggccaga agctgttcca gtcgggctgg aagcaatggg gcctggcgat gcaggacgac
    12001 atcgccgacg gcgtcaagtg ggccgtcgcc cagggcatcg tcgacccgaa gcgcgtgtgc
    12061 atcgccgggg ccagctacgg cggctatgcc gtgctgatgg gcctcgcgcg cgaccccgac
    12121 ctgtaccgct gcggcatcga gtgggtcggc gtcaccgaca tgaacctgat gtacacggcc
    12181 gactggtcgg acatgtcgga cgcgtggaag cagtacggca tgccggcgct ggtcggcgac
    12241 ccggtggccg atgcggcgca gctgaaggcg acctcgccca cccagctcgt cgcgaagatc
    12301 catgcgccgg tcctgatggc ctacggcggc aaggacaagc gcgtgccgat cgagcacggc
    12361 gagcggttcc gcgacgcgtt gaagaagcag ccgggcgcca aggtcgagtg ggtggtctac
    12421 gacaaggaag gccacggctg gcgctcgttc gagacccgcg tcgacttctg gaaccgcgcc
    12481 gcgcgcttcc tggacgccag catcggcgcg cgctgaacgc cgcgccggtc cgacaccggc
    12541 cggtcagcgc ggaaacgcct tgcgcagccg ctcgcgcgcg tcgccatagc ccgcctgggc
    12601 cttgccgcgc gcgcggtcga tgatcccgtc gccctgcagg cggcggttgc cggtcgcgcg
    12661 gccggcgagc ttcttgaggt cgcccttgac ctgcgcgatg cgcccctgga cctggtcctt
    12721 gttcatgccg gcctccccgc cacgttcagt ggaagaagaa gtacaggatg accagcacga
    12781 agaccggcac gccaagcaac caaccgacga gatacttgcc catggtgatg tctcctggag
    12841 gcggcggcgc ctcggcgggc gccgtccggg atcgaggtat tggaacgcgt gctcagctgc
    12901 ggatcaactg tccgttcgcg tcgcgccagg aatgctcgaa ggcgagcgcg tcctcggcat
    12961 tgcgcggccg cacgcccatc aggatcgctc cctcgcggat gcccgactcg tattcggcga
    13021 tgcgctcctc ggggatgccc cagccgatca gcgcgcccac cagtccgccg gccgcgccgc
    13081 ccgcacccgc gccggccagc gccgcggcga tgggcccggc gatcaccagg cccaggcccg
    13141 gcagcacgag gctggtgccc atcgcggcga cggccgcggt gatcgcgccc acggtgccgc
    13201 cgatcgcgcc gccgatgccc gcgccctcgg cggccttggt gcccagctcg gtcgcgaccg
    13261 cggcgtcggc ggggaagtga cgggcacggg tctcgtcgga catgacgagg ttgacgtcgt
    13321 cgcggtcata gccgcgcgcc gcgacggacg agtaggcgcg ctcggcgctg tcccggtcgg
    13381 gaaacaggcc ggtcacgagt cggggatctg gcatggcgct ctcctgcgaa atggatgcct
    13441 tcggccccgc ccgcaccgcg cgaacgccag gccattcgcc ctcgtttcag caagcgaggg
    13501 gcccgcacgg ccccgtcggc gccgcgccgt tcagcgccgc cggcgtcccg tcgcgatcgc
    13561 ccgcaccagc ggcaccgtca gcatcagcgc cgcggagccg gcgagcgcca tcggcacgaa
    13621 cgaatgcgtc cacgccatgg cggccgcacc ggcgaggatc gtcgcctggc cggcgagcca
    13681 caggcccagc gggccg
//
LOCUS       DATCRO010000073         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_473550, whole genome shotgun sequence.
ACCESSION   DATCRO010000073 DATCRO010000000
VERSION     DATCRO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473550"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..740
                     /locus_tag="VIN75_02035"
     CDS             279..740
                     /locus_tag="VIN75_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8972969.1"
                     /translation="MKKFLLTLALSLAAGIVAAADRPYDESADAKAQIALALRDAAAA
                     HEPVLLIFGANWCEDCRALDHALKSSRNAQLMGRFKVVKVDVGHFDHNVDVSTAYGNA
                     TVKGIPSAVIVSPQNQILFMTKAGELADARRMSEDGVYQFFTKAEAATHQQ"
     gene            complement(759..>2834)
                     /locus_tag="VIN75_02040"
     CDS             complement(759..>2834)
                     /locus_tag="VIN75_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY8972970.1"
                     /translation="LPMLAGLFGASWAWPAWVQFLLATPVQFWFGARFYAGAWRALRS
                     GGANMDVLVALGTTAAYGLSLFDWWAAGHLPHMRGMDAGHLYFEAGAVVITLVLLGKQ
                     LEARAKRQTTQALRALQALQPDMARVRRDGVDSDIAIARLRTGDRLVVRPGERFAADG
                     VVVEGESHVDESLVTGESLPVARAPGGRVTGGAINGEGLLLVDVTAVGAESTLARIVR
                     LVESAQEKKAPIQHLVDRVSAVFVPVVCAAALVTLLGWGLARGDWQRALLDAVAVLVI
                     ACPCALGLATPTALMAGTGVAARHGILIKDAEALEVAHDVSVVAFDKTGTLTEGKPML
                     VGCEAVDGDPQRLLALTAAVQSGSTHPLAQAVLDMARGTHAKVPSASAQRAVPGKGVS
                     ARVRVHADAQASAEARLAEAIAASAIMSARQSAGLSPGTGLALDAEQAIDPTGHELEL
                     RLGSARWMAELGVDLAPLAARATELQGMGRTLSWLADVTDETPRLLGLVAFGDRLRDG
                     AIEAVQRLHGQRVRTVMVSGDGRASAEGVAQLLGIEEVHAEVLPGEKASLLASLRSLA
                     TPAARRRRPVRVAMVGDGLNDAPALAAADVGIAMASGTDVAMEAAGITLMRPDPRLVA
                     DALDISRRCVAKIRQNLFWAFAYNVAGIGLAAAGLLSPMVAGAAMAFSSVSVVANALT
                     LRRWKPAGA"
BASE COUNT          414 a         1077 c          989 g          354 t
ORIGIN      
        1 gcctcacgcc cggtgtcagg tcttgaaagt tgcggtgtca ggtcttgaat cgtgcagctc
       61 ggcatggacg cacgggtgtc aggtcttgtc tcttgcatgc gaaatctcgc gcgcggcgtg
      121 ttcctacggt ggatcacgcc aaggtcagac cgggcaagcg agctcgcgag tggacgattc
      181 aagacctgac accgcaactt tcaagacctg acaccgcgat agacaccgcg atagcactgc
      241 cgccagcgcg ccggacgcga cactggcatc atggaaggat gaagaagttc ctcctgaccc
      301 tcgccctgtc gctggccgcc ggcatcgtcg ccgccgccga ccggccctac gacgaatcgg
      361 ccgacgccaa ggcgcagatc gccctggcgt tgcgggacgc ggcggcggcg cacgagccgg
      421 tgctgctgat cttcggcgcg aactggtgcg aggactgccg cgcgctggac cacgcgctca
      481 agagcagccg caacgcgcag ctgatgggca ggttcaaggt ggtgaaggtg gacgtgggcc
      541 acttcgacca caacgtcgac gtcagcaccg cctacggcaa cgccaccgtc aagggcatcc
      601 cgtcggcggt gatcgtgtcg ccgcagaacc agatcctgtt catgaccaag gccggcgagc
      661 tcgcggacgc ccgccgcatg agcgaggacg gcgtctacca gttcttcacc aaggccgagg
      721 ccgccacgca ccagcagtga cggcgccccg cgcgcgcgtc acgcgcccgc gggcttccag
      781 cgccgcagcg tcaacgcgtt ggccacgacg ctgacgctgc tgaacgccat cgccgcgccg
      841 gccaccatcg ggctcagcag cccggctgcc gccaggccga tgccggccac gttgtacgcg
      901 aaggcccaga acaggttctg gcggatcttg gcgacgcagc ggcgcgagat gtccagcgcg
      961 tcggccacca gccgcgggtc gggccgcatc agcgtgatgc cggcggcctc catggcgacg
     1021 tcggtgcccg aggccatcgc gatgcccacg tcggctgcgg ccaacgcggg cgcatcgttg
     1081 aggccgtcgc ccaccatcgc gacgcgcacc ggacgccggc gccgcgcggc cggcgtcgcc
     1141 agcgagcgca gcgacgccag caaggacgcc ttctcgcccg gcagcacctc ggcgtgcacc
     1201 tcctcgatgc ccagcagttg cgcgacgcct tcggcgctgg cgcggccgtc gccgctgacc
     1261 atcaccgtgc gcacgcgctg cccgtgcagg cgctgcaccg cctcgatcgc gccgtcgcgc
     1321 aggcggtcgc cgaacgccac caggcccagc aggcgcggcg tctcgtcggt gacgtcggcc
     1381 agccacgaca acgtgcggcc catgccctgc agctcggtgg cgcgcgcggc cagcggcgcg
     1441 aggtcgacgc ccagctcggc catccagcgg gcgctgccca ggcgcagctc gagctcgtgg
     1501 ccggtcggat cgatcgcctg ttccgcgtcc agcgcgagac ccgtgcccgg cgacaagccc
     1561 gcgctctggc gcgcgctcat gatggcgctg gcggcgatcg cctcggcgag gcgcgcctcg
     1621 gcgctcgcct gcgcgtcggc gtgcacgcgc acgcgcgcgg acacgccctt gcccggcacc
     1681 gcgcgctgcg cgctggccga cggcaccttg gcgtgcgtgc cgcgcgccat gtccagcacc
     1741 gcctgcgcca gcggatgcgt gctgccgctc tggacggcag cggtgagcgc gagcaggcgc
     1801 tgcgggtcgc cgtcgaccgc ctcgcagccc accagcatcg gcttgccctc ggtgagcgtg
     1861 ccggtcttgt cgaacgccac caccgagacg tcgtgcgcca cctccagcgc ctcggcgtcc
     1921 ttgatgagga tgccgtggcg cgccgcgacg ccggtgccgg ccatcagcgc ggtgggcgtg
     1981 gccaggccca gcgcgcacgg gcaggcgatc accagcaccg ccaccgcatc gagcagcgcg
     2041 cgctgccagt ctccccgggc caagccccag ccgagcaggg tgacgagcgc ggcggcgcag
     2101 accaccggca cgaacaccgc gctgacgcgg tcgaccaggt gctggatcgg cgccttcttc
     2161 tcttgcgcgg actccaccag ccgcacgatg cgcgccagcg tggattccgc gcccacggcc
     2221 gtcacgtcga ccagcagcag gccctcgccg ttgattgcgc cgccggtgac acgcccgccg
     2281 ggcgcgcgcg ccacgggcag gctctcgccg gtgaccagcg actcgtcgac atggctctcg
     2341 ccctcgacga cgacgccgtc ggccgcgaag cgctcgcccg gccgcacgac gaggcggtcg
     2401 ccggtgcgca ggcgcgcgat cgcgatgtca gaatcgacgc cgtcgcggcg cacgcgcgcc
     2461 atgtcgggct gcagggcctg gagcgcgcgc agcgcctgcg tggtctggcg cttggcgcgg
     2521 gcctcgagct gcttgcccag cagcaccagc gtgatgacga cggcaccggc ctcgaagtac
     2581 aggtggcccg cgtccatgcc gcgcatgtgc ggcaggtggc cggccgccca ccagtcgaac
     2641 aggctcagcc cgtacgcggc ggtggtgccc agcgcgacca gcacgtccat gttggcgccg
     2701 ccgctgcgca gcgcgcgcca ggccccggcg tagaaacggg cgccgaacca gaactgcacc
     2761 ggcgtggcca gcaggaactg cacccatgcg ggccacgccc agctggcgcc gaacaggccc
     2821 gcgagcatcg gcag
//
LOCUS       DATCRO010000074         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_754656, whole genome shotgun sequence.
ACCESSION   DATCRO010000074 DATCRO010000000
VERSION     DATCRO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754656"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIN75_02045"
     CDS             complement(<1..560)
                     /locus_tag="VIN75_02045"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8972971.1"
                     /translation="MTDDTLLSNPEAGYLLDGEPIEAARPAARRSRQARVAWGDHVVT
                     VGGDAPVRVQSMTNTDTVQVIETAIQVKELALAGSELVRITVNTPEAAAAVPHIREQL
                     DRMGINVPLVGDFHYNGHRLLTEYPACAEALSKYRINPGNVGKGAKRDTQFAQMIEVA
                     ARYDKPVRIGVNWGSLDQALLARIMD"
     gene            complement(583..1620)
                     /locus_tag="VIN75_02050"
     CDS             complement(583..1620)
                     /locus_tag="VIN75_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8972972.1"
                     /translation="MNDELTPDPTQPSAAAQPSAAAQPPGEADDAAAAAPGPATEPPP
                     QAGPATAGSMIRDARQAQGIDLASLAAMLKIPLRRLEALENDQHDELQGPTFERALAQ
                     AACRVLKIDPKPVLALLPQHARNTLEGVSEGINAPFRDGQGGGGIELPAIVRPVGVIV
                     VVLILAAIALFVVPEGWLAKVTKPFMNGLAASAPADGGAASAPAAVASAPAPLPAASA
                     AVVSPGASAAASAPVSAAPAASAALPVAATPAPAPSGVDEVLAHVAPAPTIPLQVVAT
                     ADSWVEVVDAHGRTLLSRTVVAGESVGLDGAMPMRIKVGNVSGTHLKLRGDNVDLTPW
                     TRDNVARLELK"
     gene            complement(1613..2419)
                     /gene="pilW"
                     /locus_tag="VIN75_02055"
     CDS             complement(1613..2419)
                     /gene="pilW"
                     /locus_tag="VIN75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY8972973.1"
                     /translation="MRAALAALGLLAGLAGCVTTGGPPQRPYRSNGDDDIKTASDQTN
                     ADRLYNTRMELAQAYFGRNQPTDALDQVKQALQAKPDMPGAYNLRGLIYAALGDNDKA
                     EESFRRSMQLAPHDADLIHNYGWYLCQQRQFDAADTEFRRAIAEPTNRTVPRTMLVEG
                     ICQIRAGKLPDAEKTLSRAYELDPANPAIAVNLAEVLYRGGQYERARFYVRRVNTRPE
                     LSNAQTLWLAARIEHKLGQEEQVKAMGNALHSRFPDAPETAQFEKGKFDE"
     gene            complement(2470..>3306)
                     /gene="rlmN"
                     /locus_tag="VIN75_02060"
     CDS             complement(2470..>3306)
                     /gene="rlmN"
                     /locus_tag="VIN75_02060"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8972974.1"
                     /translation="SQAGCAVGCRFCSTGHQGFSRNLTTGEIIAQLWHAEHQMRAKLG
                     LGANERAISNVVMMGMGEPLQNYSQLVPALRMMLDDHGYGLSRRRVTVSTSGVVPMID
                     RLREDCPVALAVSLHAPDDALRDQLVPINRKYPIDELLSACERYLDAAPRDFITMEYC
                     MLDGVNDTPAQARQLVSLVKDRLPVKFNLIPFNPFPESGLKRSSNARVQAFGEVLLEA
                     GLVTTIRKTRGDDIDAACGQLAGEVQDRTDAQRRMTRAIPIVAAAPSPAGKPAVPQPP
                     KQ"
BASE COUNT          427 a         1123 c         1245 g          511 t
ORIGIN      
        1 tcgtccatga tgcgagccag cagcgcctga tccaggctgc cccagttgac gccaatgcgc
       61 accggcttgt cgtagcgcgc ggccacttcg atcatttgcg caaactgcgt gtcgcgcttg
      121 gcgcccttgc ccacgttgcc cgggttgatg cggtacttgg acagcgcctc cgcgcaggcc
      181 gggtactcgg tgagcaggcg gtggccgttg tagtggaagt cgcccaccag cggcacgttg
      241 atgcccatgc ggtcgagctg ctcgcggatg tgcggcacgg cggccgccgc ctcgggcgtg
      301 ttgaccgtga tgcgcaccag ctccgagccg gccagggcca gttccttgac ctggatggcc
      361 gtctcgatca cctgcacggt gtcggtgttg gtcatcgact gcacgcgcac gggcgcgtcg
      421 ccgcccaccg tgaccacgtg gtcgccccag gccacgcgcg cctggcgcga gcggcgcgcg
      481 gcaggcctgg ccgcctcgat gggctcgcca tcgagcaagt agccggcttc ggggttggac
      541 aggagggtgt cgtcggtcat ggcaggaaac tcgtccgtgg gatcacttga gctcgaggcg
      601 ggcgacgttg tcgcgcgtcc agggcgtcag gtccacattg tcgccgcgca gcttcaggtg
      661 cgtgccgctc acgttgccga ccttgatgcg catgggcatc gcgccgtcca ggccgacgga
      721 ctcgccggcg acgacggtgc gcgacagcag cgtgcggccg tgcgcgtcga cgacctcgac
      781 ccaggaatcg gccgtggcga ccacctgcag cgggatggtg ggggccgggg cgacgtgcgc
      841 cagcacctcg tcgacacccg aaggcgcggg ggccggcgtg gctgccacgg gcagcgccgc
      901 ggacgcggcc ggcgccgcgg acacgggggc ggacgccgcc gcggacgcgc ccggcgacac
      961 caccgcggcg ctggcagccg gcagcggcgc cggtgccgaa gcgacggccg cgggcgccga
     1021 cgcggcgccg ccgtcggccg gcgcgctcgc ggccaggccg ttcatgaacg gcttggtcac
     1081 cttcgcgagc cagccctcgg ggacgacgaa cagcgcgatg gccgccagga tcagcacgac
     1141 gacgatcacg ccgaccggcc gcacgatcgc cggcagctcg atgccgccac cgccctgccc
     1201 gtcgcggaac ggcgcgttga tgccttcgga gacgccctcg agcgtgttgc gcgcgtgctg
     1261 gggcagcagc gccagcacgg gcttggggtc gatcttgagg acgcggcacg cggcttgcgc
     1321 cagcgcgcgc tcgaacgtcg ggccctgcag ttcgtcgtgc tggtcgttct cgagcgcctc
     1381 gagccggcgc agcgggatct tgagcatggc cgccagcgag gccaggtcga tgccctgggc
     1441 ctggcgcgcg tcgcggatca tgctgcccgc ggtggccgga ccggcctgcg gcggcggctc
     1501 ggtggccggc cccggggcgg cggcggccgc gtcgtccgcc tcgcccggcg gctgcgccgc
     1561 ggcggaaggc tgcgccgcgg cggaaggctg cgtcggatcg ggcgtcagct cgtcattcat
     1621 cgaacttgcc cttttcgaac tgcgccgtct cgggcgcatc cgggaagcgg ctgtgcagcg
     1681 cgttgcccat ggccttgacc tgctcttcct ggcccagctt gtgctcgatg cgcgccgcca
     1741 gccacagggt ctgcgcgttg gacagctccg gcctggtgtt gacgcggcgc acgtagaagc
     1801 gggcgcgctc gtactggccg ccgcgataga gcacctcggc caggttcacc gcgatggccg
     1861 ggttggccgg gtcgagctcg taggcgcgcg acagcgtctt ctccgcatcg ggcagcttgc
     1921 cggcgcggat ctggcagatg ccctcgacca gcatggtgcg cggcaccgtg cggttcgtcg
     1981 gctcggcgat ggcgcgccgg aactcggtgt cggccgcgtc gaactggcgc tgctggcaca
     2041 ggtaccaacc gtagttgtgg atgaggtcgg cgtcgtgcgg cgcgagctgc atcgagcggc
     2101 ggaagctctc ctcggccttg tcgttgtcgc cgagcgcggc gtagatcagg ccgcgcaggt
     2161 tgtaggcgcc cggcatgtcc ggcttggcct gcagcgcctg cttgacctgg tcgagcgcgt
     2221 cggtgggctg gttgcggccg aaataggcct gcgcgagctc catgcgcgtg ttgtacaggc
     2281 gatcggcatt ggtctggtcc gacgcggtct tgatgtcgtc gtccccgttg ctgcggtacg
     2341 ggcgctgcgg cggcccgccg gtggtcacgc accccgccag cccggccagc aggcccagcg
     2401 ccgcgagcgc ggccctcatc caacgcatcg aactggcggg ccctggaacg agatgcatgc
     2461 ggtcgtgact cactgcttgg ggggttgagg gacggcgggc ttgccggcgg gagacggcgc
     2521 cgccgcgacg atcgggatgg cgcgggtcat gcgacgctgg gcgtcggtgc gatcctgcac
     2581 ctcgcccgcg agttgaccac aggcggcgtc gatgtcgtcg ccgcgcgtct tgcggatggt
     2641 ggtgaccagc ccggcctcga gcagcacctc gccgaacgcc tgcacgcggg cgttggacga
     2701 acgcttcagg cccgactcgg ggaacgggtt gaacgggatc agattgaact tgaccggcag
     2761 ccgatccttg acgaggctca cgagctggcg cgcctgcgcc ggcgtgtcgt tgacgccgtc
     2821 gagcatgcag tactccatcg tgatgaagtc gcgcggcgcg gcgtcgaggt aacgctcgca
     2881 ggccgacagc agctcgtcga tggggtattt cctgttgatc ggcacgagct ggtcgcgcag
     2941 cgcgtcgtcg ggcgcatgca gcgacacggc cagggcgacg gggcagtcct cgcgcaggcg
     3001 gtcgatcatc ggcaccacgc ccgaggtgga caccgtcacg cggcggcgcg acaggccgta
     3061 gccgtgatcg tcgagcatca tgcgcagcgc gggcaccagc tgcgaatagt tctgcagcgg
     3121 ctcgcccatg cccatcatca cgacgttgga gatggcgcgc tcgttggcgc ccaggccgag
     3181 cttggcgcgc atctggtgct cggcatgcca cagctgggcg atgatctcgc cggtcgtgag
     3241 gttgcggctg aagccctggt gcccggtcga gcagaagcgg cagccgacgg cgcatccggc
     3301 ctgcga
//
LOCUS       DATCRO010000075         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_676008, whole genome shotgun sequence.
ACCESSION   DATCRO010000075 DATCRO010000000
VERSION     DATCRO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676008"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..174
                     /locus_tag="VIN75_02065"
                     /pseudo
     CDS             43..174
                     /locus_tag="VIN75_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(331..1677)
                     /locus_tag="VIN75_02070"
                     /pseudo
     CDS             complement(331..1677)
                     /locus_tag="VIN75_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
     gene            1990..>3683
                     /locus_tag="VIN75_02075"
     CDS             1990..>3683
                     /locus_tag="VIN75_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8972975.1"
                     /translation="MTNRSQHSRGLASPIHLAVLALLAGAAAAQAQTAAAPAGAASAA
                     KADNGELQQVTVTATKRTTTLQETPLAVTAIGAEQIEKAHVQTVSDLVHLAPSFQATT
                     EGDHGVITMTMRGIGNDSAKTEYADPEVALFVDGIYAPRAEGAAALLFDMENIEVLRG
                     PQGTLWGRNSTVGAVNMQTAKADTSGNFGSVQAGLGSYDRWGARGAFNLALSDTFAIR
                     AAFVHEQHDGYVDYQTPKLPSLADQQAAYNASNPAAGSKPFQPIDPNTFVQAGPKYNA
                     QDQSAARLSFKWKPTRDITWNASYEKFLDRGTPNMNLMQDPRPGEKFWSALISVAPYL
                     DRNVNTFRSRVDWNLSDYLTANYTAGYSDYKGSGDFDQSGGATVPTSFTTGATVQEDR
                     TNWSHYKNYSHEFQLQSRGTHAVDWILGAYYAAEDNGIRFDIPISNGTQQGNIGWQGS
                     FVQPKETVQSEAVFGQATWNATTDLHVTGGARFTWDDRKNEGGTNNAPGPTTPNYPID
                     PGTTPAQAGFTAYQHNDGHYTGNKATWLGRVNYDLAKNFLVYGSVSTGYKSGGLQDG"
BASE COUNT          628 a         1335 c         1237 g          483 t
ORIGIN      
        1 cgcttcgagc gcatcgtcga cttcctcaag ctgcactacg ccatcacgcg ccgcaccgac
       61 accgcgttct ggcgcgacaa cgccgacccg gcgtcctggc ccggctcgct gcgcgacaag
      121 ctggccctgt ggcgcagccg cccgccgcac cgcctggatt tcgtggccga tctcgagatg
      181 tacccgacgt ccagctggca gtacgtcctg tacggcatgg ggtatccgac ggacctggcc
      241 ggccgccacg ccgcgtggcc gcgcctggag gacgcgcgcc gcgagttcgc gatgatcccg
      301 caggtcgcgc agcgtggccg tccacgacgc cgggtcggcg ttgtcgcgcc agaacgcggt
      361 gtcagtgcgg cgcgtgatgg cgtagtgcag cttgaggaag tcgacgatgc gctcgaagcg
      421 cgcgctcatc atctcgttga aggacgcggc cgcgacgtcg agatcctcca gcgacggcag
      481 caggtagccc accaggtagg cgccgaactc gatcaggccg atgcccgagg attccagcgg
      541 ctcgagaaag cctgccgaca ggccgacggc cacgcagttg ccgatccacg tgcgctcgcg
      601 gtagcccacg tccagcttca gcgcgcgcgg cgccaggccc tcggccgccg ggccgatgta
      661 gccgcgcagc acgcgctcgg cgcgctcgtc gctcgtgtgg cggctggaat agacgtagcc
      721 gatgccgcgc cgctgctgca ggccgatgtc ccaggtccag ccggcctcgt gcgccgtgga
      781 gatcgtgtac gacgggatcg gcgtgtcggc ggccgggtag ggcgcctgca gcgcgagcgc
      841 gcggtcgacg aacagcacgt cgtccaggcg cctggccttc gagcccagcg tcgcgccgat
      901 cagcgcagcg cgaaagcccg tgcagtcgat gtacaggtcg gcggtgagcg cgccgctcgc
      961 gcggcccagc accgcgccga tgtggccgcg ttcgtcgagc tcgacgcgtt cgacggtgtc
     1021 cagcacgtgc gcgacgccca gcgacttcgc gtgcgtggcc agcagcgtgg cgagcttgcc
     1081 ggcgtcgaag tggtaggcgt agttcatcgg gccggcgaag tcggcgtcgc tcgcgcgctt
     1141 gggcgcgcgc gaggcgtcgg ccacgcactg ctgcatcgag accgcgtccg cgaacgcacg
     1201 cggccggccc tcgccgagca gccagtaggg cagcaactcc ggcgcgccgg gacgctggct
     1261 gggcgcgttg aacgggtgga agtagtggtt gcgcggcgtg ccgggctcgt cggcccagtc
     1321 gacgaacctc acgccctgct tgaacgtggc gtcgcactcg cggatgaagc gcgcctcgtc
     1381 gatgccgatg gccgccagcg tgccgcggat cgacgggaac gtgccttcgc cgacgccgac
     1441 gatgccgatg tcggccgact cgacgagcgt gatgcgcggc ccgccgccgg cggtgcccag
     1501 cgtcttggcg aggaatgcgg cggtgagcca gccggcggtg ccgccgccga cgatgaggat
     1561 gcgctggatc ggtgtcatgg gggatgtctc ctgcttcggc gcatcgttcg tgatcaggcc
     1621 cggagcgcct ccgcgtattc cctgatgccg gtgcgtcggc tccatatagc gcatcatatc
     1681 gacgaaaaga caacgctgtc atcttaccgt tcaattaccg ttgacagcgt tgtcatcgat
     1741 cggcatcatg cgacccattc cacctctccg atcgtccggc aacgggcgcg gagagcggcg
     1801 gagcgcggtg tcgagcacgc cgcgtgcgct gcggaatgca gcgcccgggc ccgcccccgg
     1861 gcgccgcgga agaacgaaag gcgaggaccg tgagcgcgat gccaagccgg gcatcgggac
     1921 cgcggaagaa gcctgcccca gagtcctgaa gatgacgtcg caaaagacgc gccacaaatt
     1981 ggagacaccg tgaccaaccg ctctcagcat tcccgaggcc tcgcctcccc gatccacctg
     2041 gccgtgctgg cgctgctcgc cggcgccgcc gccgcgcagg cccagaccgc cgcggccccg
     2101 gccggcgccg ccagcgccgc caaggccgac aacggcgaac tgcagcaggt caccgtgacg
     2161 gcgaccaagc gcaccaccac gctgcaggag acgccgctgg ccgtcaccgc gatcggcgcc
     2221 gagcagatcg agaaggccca cgtgcagacg gtctcggacc tggtgcacct ggcccccagc
     2281 ttccaggcca ccaccgaggg cgaccacggc gtcatcacga tgacgatgcg cggcatcggc
     2341 aacgacagcg ccaagaccga atacgccgac ccggaggtcg cgctgttcgt cgacggcatc
     2401 tacgcaccgc gcgccgaggg cgctgccgcg ctgctgttcg acatggagaa catcgaggtg
     2461 ctgcgcgggc cgcagggcac gctgtggggc cgcaactcca ccgtcggcgc cgtcaacatg
     2521 cagaccgcca aggccgacac ctccggcaac ttcggcagcg tgcaggccgg cctgggcagc
     2581 tacgaccgct ggggcgcgcg cggtgcgttc aacctcgcgc tgagcgacac cttcgccatc
     2641 cgtgccgcgt tcgtgcacga gcagcacgac ggctatgtcg actaccagac gcccaagctg
     2701 ccgtcgctgg ccgaccagca ggcggcctac aacgcgtcga acccggccgc cggctccaag
     2761 ccgttccagc cgatcgaccc gaacacgttc gtgcaggccg gcccgaagta caacgcgcag
     2821 gaccagagcg cggcgcgcct gagcttcaag tggaagccca cgcgcgacat cacgtggaac
     2881 gcgtcgtacg agaagttcct ggaccgcggc acgcccaaca tgaacctgat gcaggacccg
     2941 cgccccggcg agaagttctg gtcggcgctc atcagcgtgg ccccgtacct cgaccgcaac
     3001 gtcaacacct tccgcagccg cgtcgactgg aacctgagcg actacctgac ggccaactac
     3061 accgccggct attcggacta caagggctcg ggcgacttcg accaatccgg cggcgccacg
     3121 gtgcccacca gcttcaccac cggcgcgacg gtgcaggaag accgcaccaa ctggtcgcac
     3181 tacaagaact acagccacga gttccagctg cagtcgcgcg gcacgcacgc ggtggactgg
     3241 atcctggggg cgtactacgc cgccgaggac aacggcatcc gcttcgacat cccgatctcc
     3301 aacggcacgc agcagggcaa catcggctgg cagggctcgt tcgtgcagcc caaggagacg
     3361 gtgcaatccg aggccgtgtt cggccaggcc acctggaacg cgacgaccga cctgcacgtg
     3421 accggcggcg cgcgcttcac ctgggacgac cgcaagaacg agggcggcac caacaacgcg
     3481 cccggcccca ccacgccgaa ctacccgatc gacccgggca ccacccccgc gcaggccggc
     3541 ttcaccgcct accagcacaa cgacggccac tacaccggca acaaggccac gtggctcggt
     3601 cgcgtgaact acgacctcgc caagaacttc ctggtctacg gcagcgtgtc caccggctac
     3661 aagtcgggcg gcctgcagga cgg
//
LOCUS       DATCRO010000076         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_23315, whole genome shotgun sequence.
ACCESSION   DATCRO010000076 DATCRO010000000
VERSION     DATCRO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23315"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VIN75_02080"
     CDS             <1..351
                     /locus_tag="VIN75_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY8972976.1"
                     /translation="GGFTFFSDRVAERLAIDYTRANELEIAGGRLTGRVVPQPWGDIC
                     DGAEKRRMLLQTCRSLGILPAHAIAVGDGSNDLPMMAEAGLSIAYHAKPAVREKAMLA
                     INEGGMDDALAVLR"
     gene            complement(365..709)
                     /locus_tag="VIN75_02085"
     CDS             complement(365..709)
                     /locus_tag="VIN75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972977.1"
                     /translation="MTSELTTPDGRYIVLQGRLWRAANPTLSAERKIRYMRELLNGRR
                     ALKAAKLAGDESAITDARRMVALAQVGLGERGRVWWKDGAPDLNRTLIKNSPYAQWYA
                     SIEGDGQDAQAA"
     gene            complement(817..>2003)
                     /locus_tag="VIN75_02090"
     CDS             complement(817..>2003)
                     /locus_tag="VIN75_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEY8972978.1"
                     /translation="ELLEVLLKAVRERTQIAVQRNDFKLETLSPHHFMNFRVVDEHGR
                     QLGMGRNLAALKSELGGQARSAFQALAALKLPAAAQAPAAPEPGAPAPSGKGIVARVA
                     TPPPPPAMQAATKYTTWSFGQLPELMEIAKGGQTLIGFPALIDKGAHVEIEVFDEPDV
                     AAAKHRAGLRRLLALQLKEPIRFLERNIPDLTKMSVLYMPLGTAEELREQIIDVAIDR
                     AFLEDPLPTDEGEFRRRLEQGRSRLNLIAQEVARQGSAVLVEYAAATRKLKDARPPKE
                     VVDDIAAQLQRLMGKRFIQATPWAQLQHLPRYLKGITLRLDKLRADPARDSQRLAELR
                     PVEQRYLRTLAERKGAVDARLTEYRWLLEELRVSLFAQELRTPQPVSVKRLDRAWEQL
                     TR"
BASE COUNT          267 a          721 c          700 g          315 t
ORIGIN      
        1 ggcggcttca ccttcttcag cgaccgcgtg gccgagcgcc tggcgatcga ctacacgcgc
       61 gccaacgagc tcgagatcgc cgggggccgc ctcaccggcc gcgtggtgcc gcagccctgg
      121 ggcgacatct gcgacggcgc cgaaaagcgc cgcatgctgc tgcagacgtg ccgttcgctg
      181 ggcatcctgc ccgcccacgc gatcgccgtc ggcgacggtt cgaacgacct gccgatgatg
      241 gccgaggccg gcctgtcgat cgcgtaccac gccaagccgg cggtgcgcga gaaggccatg
      301 ctggccatca acgaaggcgg gatggacgac gcgctggccg tgctgcgctg agcgaacgtc
      361 cgggtcaggc cgcctgcgcg tcctggccgt cgccctcgat cgacgcgtac cactgcgcgt
      421 agggcgagtt cttgatgagc gtgcggttga ggtcgggcgc cccgtccttc caccacacgc
      481 gaccgcgttc gccgaggccg acctgggcca gggcgaccat ccggcgggcg tcggtgatgg
      541 ccgactcgtc gccggccagc ttggccgcct tcagcgcgcg ccggccgttg agcagctcgc
      601 gcatgtagcg gatcttgcgc tcggccgaga gcgtcgggtt cgccgcgcgc cagaggcgcc
      661 cttgcagaac gatatagcgg ccatccggcg tcgtcagctc cgaggtcatt ccgggaatct
      721 agcggacgcg gcgaccggcc ttgatgcgaa acgctaccgg aataccccct cgcgaaacag
      781 cttgatacag cctcgtcggg ggatccaggt ttcccctcag cgggtcagct gttcccaggc
      841 cctgtccagc cgcttgacgc tgaccggctg cggcgtgcgc agctcctgcg cgaacaggct
      901 cacgcgcagt tcctcgagca gccagcggta ctcggtcaat cgcgcgtcga cggcgccctt
      961 gcgctcggcc agcgtgcgca ggtagcgttg ttccaccggc cgcagctcgg cgaggcgctg
     1021 gctgtcgcgg gccgggtcgg cgcgcagctt gtccagccgc agcgtgatgc ccttgaggta
     1081 gcgcggcagg tgctgcagct gggcccacgg cgtcgcctgg atgaagcgct tgcccatcag
     1141 gcgctgcaac tgcgccgcga tgtcatcgac cacctccttg ggcggccgcg cgtccttgag
     1201 cttgcgcgtg gccgccgcgt actcgaccag caccgcgctg ccctgccgcg ccacttcctg
     1261 cgcgatcagg ttcaggcggc tgcgcccctg ctccaggcgg cgcctgaact cgccttcgtc
     1321 ggtgggcaac gggtcctcca ggaacgcgcg gtcgatcgcg acatcgatga tctgctcgcg
     1381 cagctcctcg gccgtgccca gcggcatgta cagcacggac atcttcgtga ggtcggggat
     1441 gttgcgctcg aggaagcgga tgggctcctt gagctgcagc gcgagcagcc ggcgcagtcc
     1501 ggcgcggtgc ttcgccgcgg cgacatcggg ctcgtcgaac acctcgatct cgacgtgcgc
     1561 gcccttgtcg atgagcgccg ggaagccgat cagcgtctgg ccgcccttgg cgatctccat
     1621 cagctcgggc agctggccga acgaccacgt cgtgtacttc gtggcggcct gcatcgcggg
     1681 cggaggcggc ggcgtcgcca cgcgcgcgac gatgcccttg cccgacggcg cgggcgcgcc
     1741 cggctcgggc gcggcgggtg cttgcgccgc cgcgggcagc ttcagcgcgg ccagggcctg
     1801 gaacgcggag cgcgcctgcc cgcccagctc ggacttcagc gccgccaggt tgcgccccat
     1861 gcccagctgg cggccgtgct cgtcgaccac gcggaaattc atgaagtggt gcggcgacag
     1921 cgtctccagc ttgaaatcgt tgcgctgcac cgcgatctgc gtgcgctccc tgaccgcctt
     1981 gagcagcacc tccagcagct cgc
//
LOCUS       DATCRO010000077         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1070, whole genome shotgun sequence.
ACCESSION   DATCRO010000077 DATCRO010000000
VERSION     DATCRO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>2465
                     /locus_tag="VIN75_02095"
     CDS             15..>2465
                     /locus_tag="VIN75_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEY8972979.1"
                     /translation="MIFADGDLRLAGASLLNENSTIEASRALALQMTGGIVNRTVLPG
                     VNTGSSAGTVTGGGSTGGGGKPGELTETDTASEGGEGVGGSISSGATALGNLTSKAFI
                     RAGGDMTISGDTLLNSGATIEARSNLTLTANRIDNVNPYLMWGNYTPGSEGSLSVLVS
                     MNGGPVEDIFVPLNGRTAQEAVNNYARSHPTHRIISWQEFGGSDAGAPVTQSLAAKII
                     VGGTLSIGAGTINNDMSQVVGMTGVAITGGQVTNVPHTVTATDAQGVAHTVNLALAGG
                     APVVGQVSQAPGGRQGAGAVSTGGGASTQQAQGGASANASGGAGTGGLLGRVTRQVQQ
                     GIAAQANAQGNAQVRAGGAIASNAVATGNGAAGAGAVQAIQRSGATATERAIAAAQAG
                     DAGAQVQATMRHERDGDAVQTGSAQDLQGARVTTQFRDGQAVDAAQGNGTTQQVQGTA
                     RTGLAGLLQRARAAAAAQVDASAQSDQKATTQIGKVTTQAGSTSRPGSAPSAALKVSG
                     LSVGGRVVGVMPNLTLPSNSLFKTHSEPGSKYLVETDPRFANYREWLSSDFLLEAMAL
                     DPTVTQKRLGDGFYEQRLVREQIGQLTGTGYLSGYADDESMYRALLSNGATFAKEHQL
                     VPGVALTAEQMAQLTSDLVWLVEQTVTLADGTTQRVLVPQVYLVPREGDLLLSGSLIA
                     GGRVEMALTGDFNNGGSVRGGVVAIQAQNIANTGDMRATTLALTAREDLRNIGGQLAA
                     TGDMSLVAGRDVVMQSTTASGGTVKGNVTTKATVLDQVASLTAGGVMVVQAGRDAQLQ
                     AVQITQGGADGAGAEGGVM"
BASE COUNT          417 a          798 c          960 g          290 t
ORIGIN      
        1 acacggacgg ggcattgatc ttcgcggacg gcgatctgcg gctcgcgggc gcgagcctgc
       61 tcaacgagaa ctcgaccatc gaggccagcc gtgcgctggc gctgcagatg acgggtggga
      121 tcgtgaatcg gacggtgctg cccggggtca ataccggctc gtcagcaggg accgtgacgg
      181 ggggcggcag cactggtggt ggcggcaagc ctggggagct cacggagacc gacaccgcga
      241 gcgaaggcgg cgaaggagtc ggcgggtcga tcagcagcgg ggccaccgcg ctgggcaacc
      301 tcacgtcgaa ggccttcatc cgtgcgggcg gggacatgac gatctccggc gacacgctgc
      361 tcaacagcgg cgccacgatc gaggcgcgca gcaacctgac gctgacggcc aatcgcatcg
      421 acaacgtcaa cccgtacctg atgtggggca actacacgcc gggctcggag ggctccctga
      481 gcgtgctcgt ttcgatgaat ggcgggccgg tcgaggacat ctttgttccg ctgaacgggc
      541 gcaccgcaca ggaggcggtg aacaactatg cgcggtcgca tcccacccat cggatcattt
      601 cctggcagga gttcggcggc agtgacgcag gggcgccggt gacgcaatcg ctggcggcca
      661 agatcatcgt tggcggcacc ttgagcatcg gtgcgggcac gatcaacaac gacatgagcc
      721 aggtggtcgg catgaccggc gtggcgatca ccggtgggca ggtcacgaac gtgccgcaca
      781 ccgtgaccgc cacggacgcg cagggcgtgg cgcacacggt gaacctggcg ctggcgggcg
      841 gtgcgccggt ggtggggcag gtgtcgcagg cgccgggcgg gcgtcagggc gccggtgcgg
      901 tgagcacggg aggcggtgcc tcgacacagc aggcgcaggg cggtgcttcc gccaacgcga
      961 gcggtggcgc cggtaccggc ggtctgctcg gccgcgtgac tcgccaggtg cagcaaggca
     1021 ttgccgcgca ggccaacgcg caaggcaatg cgcaggtgcg ggcgggcggc gcgatcgcgt
     1081 cgaacgccgt ggcgacgggc aacggcgccg caggcgccgg cgccgtgcag gccatccagc
     1141 gcagcggcgc gaccgcgacc gagcgcgcca tcgccgccgc gcaggccggc gacgcgggcg
     1201 cacaagtgca agcgacgatg cgtcacgagc gcgatggcga cgcggtgcag acgggctcgg
     1261 cgcaagacct gcaaggcgca cgcgtgacca cgcagttccg cgatgggcag gcggtcgacg
     1321 ccgcgcaggg caacggcacg acgcaacagg tgcagggcac ggcgcgaacc gggctcgccg
     1381 gcttgcttca gcgggcccgt gccgcggcgg ccgcgcaggt cgacgcgtcg gcgcagtccg
     1441 accagaaggc gaccacacag atcggcaagg tgacgaccca agccggctcg acgagccgac
     1501 ccggctcggc gccgagcgcg gcgctgaagg tctcgggtct gagcgtcggt ggccgcgtgg
     1561 tcggcgtgat gcccaacctc acgctgccgt ccaacagcct gttcaagacg cacagcgagc
     1621 cgggcagcaa gtacctggtc gagaccgacc cgcgcttcgc caactaccgc gaatggctct
     1681 ccagcgactt cctgctggag gcgatggcgc tggacccgac ggtgacgcag aagcgcctgg
     1741 gcgacggctt ctacgagcag cgcctggtgc gcgagcagat cggccaactg accggcacgg
     1801 gctacctgtc gggctacgcc gacgacgagt cgatgtaccg cgcgctgctg agcaacggcg
     1861 cgaccttcgc caaggagcac caactggtgc cgggcgtggc gctgaccgcc gagcagatgg
     1921 cgcagctcac cagcgacctg gtgtggctgg tggagcagac ggtgacgctg gcggacggca
     1981 cgacgcagcg cgtgctggtg ccgcaggtct acctggtgcc gcgcgaaggg gacctgctgc
     2041 tcagcggctc gctgatcgcg ggcggccggg tggagatggc gctgacgggc gacttcaaca
     2101 acggcggcag cgtgcgcggc ggcgtggtgg ccatccaggc gcagaacatc gccaacaccg
     2161 gcgacatgcg cgccacgacg ctggcactga cggcgcgcga ggacctgcgc aacatcggcg
     2221 ggcagctcgc ggccacgggc gacatgagcc tggtggcggg gcgtgacgtc gtcatgcaga
     2281 gcaccaccgc cagcggcggc acggtgaagg gcaacgtcac gacgaaggcg acggtgctcg
     2341 accaggtggc gtcgctcacc gcggggggcg tgatggtggt gcaggccggg cgcgacgcgc
     2401 agctgcaggc ggtgcagatc acgcagggcg gcgcggacgg cgcgggcgcc gaaggcggcg
     2461 tgatg
//
LOCUS       DATCRO010000078         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_180869, whole genome shotgun sequence.
ACCESSION   DATCRO010000078 DATCRO010000000
VERSION     DATCRO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180869"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VIN75_02100"
     CDS             <1..908
                     /locus_tag="VIN75_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972980.1"
                     /translation="INPRLWGSLPLTIAAGVPIPRGLLSLAVGEPLAPLRGWRVGVTA
                     RNLSNDIQWFIDNLRADRRDPMLLTTSPWRAALGWLRVLTGRESWDGWSWRDTAIARE
                     ELSHLLHKRFAGLAERVARRAAQSRARKHHASLRRRDGALARPLGSVLFLCLGNLCRS
                     PFAEVAAAQRLAGVAIASAGFLSHDGRPSPPHVVQVAQTLGVDLSRARAHRVTAAEVE
                     RADLVVCMDVTHLERMAREFPQAMRKTTLLGLFHAGGPIEMTDPYDLSPAATRAVFEQ
                     MLRAIDALTAQAPRALADAAPPEPA"
     gene            complement(916..2049)
                     /locus_tag="VIN75_02105"
     CDS             complement(916..2049)
                     /locus_tag="VIN75_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8972981.1"
                     /translation="MAILFWASLLLIIHPYVTYPLTLRIWRALRPRPVQRAPHEPPVS
                     VLIPAYNEVEVIAATIDALLLQDYPKDKLQILVVSDCSDDGTDDVVRGYADRGVELLR
                     QAERGGKALGLNAAVRQARGDIVIFCDANARFAPGAVRSFAQNFADPSVGYVTGQLEL
                     EAAGDVIAGGGAYLRFEEWLRASESAVASVIGVNGGCDAIRRELYSDIPRELITDFVL
                     PLRVIAGGHRVVLDAQAKSRETANTELQAEFSMRVRVALRALQGLVHMRRLLNPLRYP
                     AAAYCLVSHKVVRYLAYVFLVTALLSNLVLAASQPVYQVLLLGHLACYALAACGLLGI
                     GRKQLGIACVAPAYLLVSYWAFAIASWRFLRGQTMATWRPRAG"
     gene            complement(2064..3515)
                     /locus_tag="VIN75_02110"
     CDS             complement(2064..3515)
                     /locus_tag="VIN75_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972982.1"
                     /translation="MARRLDLWLPSYVLGAAARARARRERRGKLTHVFFLICDHYEPR
                     HKATREGQPQERVATWRRDYPAFQQRCIDAFGHAPLHSWFYPPHHGAEHLPELSAMAH
                     AGGGEIELHYHHSNDTAETLTRDLKATLALYHRHGLLLESGEPPRTAFGFVHGDWTLD
                     NACNGAYCGVNGELSLLAGLGCWGDFTMPSANEAQTRKINSIYYAEDSPGRCKSHDHG
                     IDAAVGHAGRPGFWLMQGPLAVNWRAPGYPRIENASITTPNWGRPDRVRSWLDCGVHV
                     KGRPEWLFIKLHTHGAVERDFDALFGDKAFQMHRTLNELCNDGRDYQLHYVTARQAYN
                     LAKAAERGLGGDPRQWLDLVVDKPATAHYALPQPHDLAACTARRLRLSNVDAGGEATL
                     STTIGALRELRGAFCAIDLDGGDAATPGSIAIEPRGAGQMQLAFRRPVELEAESGGAR
                     VAAIDAEGTRWRIEPAAGGLLKLRYRERATVAA"
     gene            complement(3515..4726)
                     /locus_tag="VIN75_02115"
     CDS             complement(3515..4726)
                     /locus_tag="VIN75_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8972983.1"
                     /translation="MRVLYHHRTQGEEPESIHIESIVAALRALGHEVRIVGPVDIEHR
                     EASSAATTAAKARRPSRLVRIKESVPRSIFELLQIAYNAVAWRRLDRAVREYQPDFIY
                     ERYALYAFAGGMVARRHGIPLILECNTPYAQAWAKYYGLHLPRLARWLEARTLRGADH
                     VITVTHAQGKLLQQIGVAPERLTVSHNAIDPAWFNQERQHDPALRERLGLKGLVIGFV
                     GTMNRWQGIPKFAEVIPSVLARHPDASFLFVGDGEFRQPLEDLCREAGTLDRVKFVGR
                     QRHDKVPAFVAAMDIAVLLDSNAYGSPMKIFEYWGMGKPVVAPSVVPVLEVMRDRETG
                     LLIEPGNAAQLADRIVELAGDPALRARLGAAGRACATAHHTWRNNAEQIVAAHAALTE
                     VPGDSPVKVRP"
     gene            4965..>5842
                     /locus_tag="VIN75_02120"
     CDS             4965..>5842
                     /locus_tag="VIN75_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8972984.1"
                     /translation="MASLSDKAGFLIGANLVKYAVGFITPMVLVRMLDRADYGTYQQL
                     ALIGALGIAVLVLGLPNSVYYFYDRKNPARDKVLTLQTSAILLASGLLTAIVLLVARP
                     LVVHLMGNEAISGPMPWYVMALGLLISTEHFVQFMIARDRYKVAVLTEVVETIIRVLI
                     LVLPLLAGYGLMGLVTSLFAYAFIRFVVRTAWLVWPLGGVGKPAGEHWFMREQLEYAV
                     PLGLMSLVGLIGGYLDRGIVATRFSPSDYAIYSVGALEIPLDAIFQASVATVLRASMP
                     ALIRDGRHDEVIRLLR"
BASE COUNT          788 a         2035 c         2138 g          881 t
ORIGIN      
        1 agatcaatcc gcgcctgtgg ggttcgctgc cgctgacgat cgcggccggc gtgccgatcc
       61 cgcgcgggct gctgtcgctg gccgtgggcg agccgctcgc gccgctgcgc ggctggcgcg
      121 tgggcgtgac ggcgcgcaac ctgtccaacg acatccagtg gttcatcgac aacctgcgcg
      181 cggaccggcg cgacccgatg ctgctgacga cctcgccctg gcgcgcggcg ctgggctggc
      241 tgcgcgtgct gaccggccgc gagagctggg acggctggtc gtggcgtgac accgccatcg
      301 cgcgcgagga gttgtcgcac ctgctgcaca agcgcttcgc gggcctggcc gagcgcgtcg
      361 cgcggcgggc ggcgcagtcg cgggcgcgca agcatcacgc gtcgctgcgg cgccgcgacg
      421 gcgcgctcgc gcggccgctc ggctcggtgc tgttcctgtg cctgggcaac ctgtgccgca
      481 gcccgttcgc cgaggtcgcg gcggcccagc gcctggccgg cgtggcgatc gccagcgcgg
      541 gcttcctgtc gcacgacggc cgcccgagcc cgccgcacgt ggtgcaggtg gcccagaccc
      601 tgggcgtcga cctgtcgcgg gcgcgcgcgc accgggtcac ggcggccgag gtcgagcggg
      661 ccgacctggt cgtctgcatg gacgtgacgc acctggaacg catggcgcgc gagttcccgc
      721 aggcgatgcg caagaccacg ctgctgggcc tgttccacgc gggcggcccg atcgagatga
      781 ccgatcccta cgacctgtct ccggcggcca cgcgcgccgt gttcgagcag atgctgcgcg
      841 cgatcgacgc gctcaccgcg caggcgccgc gggcgctggc ggacgccgct ccgcccgagc
      901 cggcctgagc cggcctcagc ccgcgcgcgg gcgccaggtg gccatcgtct ggccgcgcag
      961 gaagcgccag ctcgcgatgg cgaaggccca gtagctgacc agcaggtagg ccggcgcgac
     1021 gcacgcgatg cccagctgct tgcggccgat gcccagcagg ccgcaggcgg ccagcgcgta
     1081 gcaggccagg tgccccagca gcagcacctg gtagaccggc tggcttgcgg ccagcacgag
     1141 attggacagc agcgccgtca ccaggaacac gtaggcgagg tagcgcacca ccttgtgcga
     1201 gaccaggcag taggcggccg cgggatagcg cagcgggttg agcagccggc gcatgtgcac
     1261 caggccctgc agcgcgcgca gcgcgacgcg cacgcgcatc gagaactcgg cctgcagctc
     1321 ggtgttggcc gtctcgcgcg actttgcctg ggcgtcgagc accacgcggt ggccgccggc
     1381 gatcacgcgc agcggcagca cgaagtcggt gatgagctcg cgcgggatgt cagagtacag
     1441 ctcgcggcgg atcgcgtcgc agccgccgtt gacgccgatg accgacgcga ccgcgctctc
     1501 ggaggcgcgc agccattcct cgaagcgcag gtaggcgccg ccgccggcga tgacgtcgcc
     1561 cgcggcctcg agctcgagct ggccggtgac gtagcccacc gacggatcgg cgaagttctg
     1621 cgcgaagctg cgcactgcgc cgggcgcgaa gcgggcgttg gcgtcgcaga agatgacgat
     1681 gtcgccgcgc gcctggcgca cggcggcgtt cagccccagc gccttgccgc cgcgctcggc
     1741 ctggcgcagc agctccacgc cgcggtcggc gtagccgcgc acgacgtcgt cggtgccgtc
     1801 gtccgagcag tcggacacca ccaggatctg cagcttgtcc ttggggtagt cctgcagcag
     1861 cagcgcgtcg atcgtggcgg cgatcacctc gacctcgttg tacgccggga tcagcacgct
     1921 gaccggcggc tcgtgcggcg cgcgctgcac ggggcgcggg cgcagcgcgc gccagatgcg
     1981 cagcgtcagc gggtacgtga cgtacggatg gatgatgagc agcagagacg cccagaagag
     2041 gatggccatg cgagaagttc ctgtcaggcg gcgacggtgg cgcgttcgcg atagcgcagt
     2101 ttgagcaggc cgccggcggc cggctcgatg cgccaccggg tgccttcggc gtcgatggcg
     2161 gccacgcgcg ccccgccgga ctcggcctcg agctcgaccg gccgccggaa cgccagctgc
     2221 atctggccgg cgccgcgcgg ctcgatggcg atcgagcccg gcgtcgcggc gtcgccgccg
     2281 tcgaggtcga tcgcgcagaa cgcaccgcgc agttcacgca gcgcgccgat ggtggtcgac
     2341 agcgtcgcct cgccgccggc gtcgacgttg gacaggcgca gccgccgcgc ggtgcaggcg
     2401 gccaggtcgt gcggctgcgg cagggcatag tgcgcggtgg cgggcttgtc gacgacgagg
     2461 tcgagccact ggcgcgggtc gccgcccagg ccgcgctcgg cggccttggc gaggttgtag
     2521 gcctggcgcg cggtgacgta gtgcagctgg tagtcgcggc cgtcgttgca cagctcgttg
     2581 agcgtgcggt gcatctggaa ggccttgtcg ccgaacagcg cgtcgaagtc gcgctccacg
     2641 gcgccgtgcg tgtgcagctt gatgaacagc cactccgggc ggcccttcac gtgcacgccg
     2701 cagtccagcc atgaacgcac gcggtccggg cggccccagt tgggcgtggt gatgctcgcg
     2761 ttctcgatgc gcggatagcc cggcgcgcgc cagttgacgg ccagcgggcc ctgcatcagc
     2821 cagaagccgg ggcgcccggc gtggccgacc gcggcgtcga tgccgtggtc gtgcgacttg
     2881 cagcgcccgg ggctgtcctc ggcgtagtag atcgagttga tcttgcgcgt ctgcgcctcg
     2941 ttggcggacg gcatcgtgaa gtcgccccag cagcccaggc ccgccagcag gctcagctcg
     3001 ccattgacgc cgcagtaggc gccgttgcac gcgttgtcga gtgtccagtc gccgtgcacg
     3061 aagccgaacg cggtgcgcgg cggctcgccc gactccagca gcaggccgtg gcggtggtag
     3121 agcgccagcg tcgccttcag gtcgcgcgtg agcgtctcgg cggtgtcgtt gctgtggtgg
     3181 tagtgcagct cgatctcgcc gccgccggcg tgcgccatcg ccgacagctc gggcaggtgc
     3241 tcggcgccgt ggtgcggcgg gtagaaccag ctgtgcagcg gggcgtggcc gaaggcgtcg
     3301 atgcagcgct gctggaacgc cggatagtcg cgtcgccacg tggccacgcg ttcctgcggc
     3361 tggccttcgc gcgtggcctt gtggcgcggt tcgtagtggt cgcagatcag gaagaagacg
     3421 tgcgtcagct tgccgcgccg ctcacgcctg gcgcgggcgc gcgccgcggc tccgagcacg
     3481 tagctcggca gccagaggtc gagtcgtcgg gccatcaggg gcgcaccttc acgggcgaat
     3541 ctccaggaac ttcggtcaga gcggcgtgcg cggcgacgat ctgctcggcg ttgttgcgcc
     3601 aggtgtggtg cgccgtcgcg cacgcgcgcc cggccgcgcc caggcgcgcg cgcaaggccg
     3661 gatcgccagc cagctcgacg atgcggtcgg cgagctgcgc cgcgttgccg ggctcgatca
     3721 gcaggcccgt ctcgcggtcg cgcatcacct cgagcaccgg caccacgctg ggcgccacca
     3781 cgggcttgcc catgccccag tactcgaaga tcttcatcgg cgagccgtac gcattggaat
     3841 cgagcagcac ggcgatgtcc atggccgcga cgaacgcggg caccttgtcg tggcgctggc
     3901 ggccgacgaa cttcacgcga tccagcgtgc ccgcctcgcg gcacaggtcc tccagcggct
     3961 ggcggaactc gccgtcgccc acgaacagga agctggcgtc gggatggcgc gcgagcacgc
     4021 tcgggatgac ttcggcgaac ttggggatgc cctgccagcg gttcatcgtc ccgacgaatc
     4081 cgatgaccag gcccttcagg cccaggcgct cgcgcagcgc cggatcgtgc tggcgctcct
     4141 ggttgaacca cgccgggtcg atcgcgttgt gcgacaccgt caggcgctcc ggcgcgacgc
     4201 cgatctgctg cagcagcttg ccctgcgcgt gcgtcaccgt gatgacgtgg tcggccccgc
     4261 gcaaggtgcg cgcctcgagc cagcgcgcca gccgcggcag gtgcaggccg tagtacttgg
     4321 cccaggcctg cgcgtagggc gtgttgcact cgaggatcag cgggatgccg tggcggcgcg
     4381 cgaccatgcc gccggcgaac gcgtacagcg catagcgttc gtagatgaag tcgggctggt
     4441 actcgcgcac ggcgcgatcc aggcggcgcc acgcgaccgc gttgtacgcg atctgcagca
     4501 gctcgaagat gctgcgcggc accgattcct tgatgcggac gagccggctc ggcctgcgcg
     4561 ccttggcggc cgtcgtcgcg gcgctcgacg cttcccgatg ctcgatgtcg accggcccga
     4621 cgatgcgcac ttcgtgcccc agcgccctga gagcggccac gatggactcg atgtggatcg
     4681 actccggctc ttcgccctgg gtccggtggt gatacagaac cctcatctga cttcccactc
     4741 ctgccgctag tttggcacac gacacccggg aaaagcgcga aaaacggcca agctccccca
     4801 agatggtgat acgtttgccg caacatttgc gcggtgctcg cataaactgg acgccttcag
     4861 aacaccggcc gggctggtgc ggcgacgagt ccgcgcggca gaagggtcca ccggcgcccg
     4921 gcgtcggcga ggacacgcgc cgcacgaggg atttcacagc tgcgatggca tcgctttccg
     4981 acaaggccgg ttttctcatc ggcgccaacc tggtcaagta cgcggtcggc ttcatcacgc
     5041 cgatggtgct cgtgcgcatg ctggatcgcg ccgactacgg cacctaccag cagctcgcgc
     5101 tgatcggtgc gctgggcatc gcggtcctgg tgctgggcct gcccaattcg gtctactact
     5161 tctacgaccg caagaacccg gcgcgcgaca aggtgctgac gctgcagacg tcggcgatcc
     5221 tgctggccag cggcctgctc accgccatcg tgctgctggt cgcgcgtccg ctcgtggtgc
     5281 acctgatggg caacgaggcg atctcggggc cgatgccgtg gtacgtcatg gcgctggggc
     5341 tgctgatctc caccgagcac ttcgtgcagt tcatgatcgc gcgcgatcgc tacaaggtcg
     5401 ccgtgctcac cgaggtggtc gagacgatca tccgcgtgct gatcctcgtg ctgccgctgc
     5461 tcgcgggcta cggcctgatg gggctggtga cctcgctgtt cgcgtacgcg ttcatccgct
     5521 tcgtcgtgcg caccgcgtgg ctcgtctggc cgctcggcgg cgtcggcaag ccggccggcg
     5581 agcactggtt catgcgcgag cagctcgagt acgcggtgcc gctgggcctg atgtcgctgg
     5641 tgggcctgat cggcggctac ctcgaccgcg gcatcgtggc cacgcgcttc tcgccgtcgg
     5701 actacgccat ctactcggtc ggcgcgctcg agatcccgct cgacgcgatc ttccaggcct
     5761 cggtggccac cgttctgcgg gcgtcgatgc cggcgctgat ccgcgacggc cgccacgacg
     5821 aggtcatccg cctgctgcgc ga
//
LOCUS       DATCRO010000079         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_619841, whole genome shotgun sequence.
ACCESSION   DATCRO010000079 DATCRO010000000
VERSION     DATCRO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619841"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1136
                     /locus_tag="VIN75_02125"
                     /pseudo
     CDS             93..1136
                     /locus_tag="VIN75_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
     gene            1146..3575
                     /gene="xdhB"
                     /locus_tag="VIN75_02130"
     CDS             1146..3575
                     /gene="xdhB"
                     /locus_tag="VIN75_02130"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY8972985.1"
                     /translation="MNKEVETFLVEPGLAYADYLRNARSRIDADGEAQREREGARVGI
                     SRRHESAHLHVAGRATYVDDMPELAGTLHAALGLSPVAHGRLKGIAVDRIRALPGVVA
                     VITAADIPGVNDCGAIVHDDPILADGHVHYLGQPVFAVIATDRELARRAAGQARQFLD
                     IEPLPALITTAEAHAAQQYVLPPMHLERGDAKAAIAAAPRHLKGTISLGGQEQFYLEG
                     QISYAIPTENDGMRVHCSTQHPSEMQHLVAHALRLDSHHVHVECRRMGGGFGGKESQS
                     GLFACIAAIAARQLDRPVKMRPDRDDDFMITGRRHGFEYSYEVGFDDAGRVLGAEIDM
                     VSNAGFSTDLSGPVMTRALCHFDNAYWLPDVAIHGYSARTNTQSNTAFRGFGGPQGAF
                     AAEVILDSIARALGKDALEVRRVNFYGIGERDVTPYGQRVVDNVIRPLTDELAAKADY
                     AQRRARINAFNATSPVLKKGLALTPVKFGISFNVKHFNQAGALVHVYADGTMLVNHGG
                     TEMGQGLNTKVAQVVAHELGVSFDTVRATATDTTKVANTSATAASTGADLNGKAAQDA
                     ARRIRERLADFAARRHGGDAADVRFANDVCHVNGHAIPFPELARAAYEDRVQLWSDGF
                     YATPGLHWDRVKLQGRPFFYFAYGAALSEVVVDTLTGEWKLLRADVLHDVGRSLNPAV
                     DIGQVEGAFIQGMGWLTMEELVWHPKTGLLTTHAPSTYKIPTANDCPPVFNVALYDNA
                     NVEDSIHRSKAVGEPPLLLPFSVFLAIRDAVSALGDHRVDPPLRAPATGEAIMDAVDA
                     VRRGLAGKEAS"
     gene            3634..>4261
                     /gene="xdhC"
                     /locus_tag="VIN75_02135"
     CDS             3634..>4261
                     /gene="xdhC"
                     /locus_tag="VIN75_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY8972986.1"
                     /translation="MSAALHATAARWLAAGEPGVVIEVVKTAGSVPRETGTRMLVSAT
                     QAVGTIGGGHLELMAIGNARTLLAHVPDFAPVDRHYPLGPALGQCCGGAVTLRFARLD
                     AATVRAWPLVTPRFELQLYGAGHVGRAIVKALSGIACRVQWIDEREDEFPADTALPPH
                     IEKVCVDPVEAEVDVAPANSCFLVLTHRHDLDMRIAEAILRRGDFRYLG"
BASE COUNT          623 a         1604 c         1467 g          567 t
ORIGIN      
        1 tcgatgcgct tgagcgcgtc gacgttgccg atgtagagga tgtcgcccag gtcgcggaac
       61 tgcttgttga cccacagccc gatgtcggtg ctgccggcca gcgcatgttc gacctgcgca
      121 ccgcgcgcct ggacacgcgc cccatcgtcg aggcgctgcg caagctcgat gccgcggacg
      181 gcctggccta cgaaggcgac aaccccgccg tgcgcaccca cgccggcccg cgccgcgacc
      241 gcttctgggc cccccgcgcg ctcgacgact tcgccgcgct gcgcgagcag catccgcagg
      301 cccgcgtcct cgccggttgc accgacatcg gcctgtgggt caacaagcag ttccgcgacc
      361 tgggcgacat cctctacatc ggcaacgtcg acgcgctcaa gcgcatcgag cgccgcggcg
      421 gcgtcctgcg catcggcgcc gcggcctcgc tggaggacgc ctggtccacg ctcaccgcgc
      481 aggtgcccga gctgcacgac atgtggctgc gttttgcatc gctgccggtg cgcaacgccg
      541 gcacgctggg cggcagcatc gccaacggct ccccgatcgg cgacagcgcg ccggtgctga
      601 tggcgctcgg cacggacgtc gtgctgcgcc gcggagccgc cacgcgcacg ctgcggctgg
      661 aggacttcta cctggactac atgaagaacg cgctgcagcc gggcgagttc atcgaggcgc
      721 tggaggtggc gctgcccgcg cccgccgcgg ccgtgcgcgc ctacaagctg tccaagcgct
      781 tcgactgcga catctcggcc gtcgccgccg gcctggtgct cgagctcgac ggcgatcgcg
      841 tgcgcacggc gcgcttcgcc ttcggtggca tggcggcgat cgtcaagcgc gcggctggcg
      901 ctgaagccgc ggtcgtcggg cagccctgga cggaagcgac catcgaggcc gcaatggccg
      961 cgctggcctg cgacttcacg ccgctgaccg acatgcgcgc cagcgccgcc caccgcgcgc
     1021 gcgtggcggc caacctgctg cgccgcttct ggctggagac acgggccgac gcgccgctgc
     1081 ccgcgcgcag caccagcgtc tttcacgtga tgccgcacgc ggcaccggcc gcctgagggg
     1141 atcacatgaa caaggaagtc gaaacgttcc tcgtcgagcc gggcctcgcc tacgccgact
     1201 acctgcgcaa cgcgcgctcg cgcatcgatg ccgacggcga agcccagcgc gagcgcgagg
     1261 gcgcgcgcgt gggcatcagc cgcaggcacg agtcggcaca cctgcacgtc gccggccgcg
     1321 cgacctacgt cgacgacatg cccgagctgg ccggcacgct gcacgcggca ctgggcctgt
     1381 cgccggtggc gcacggccgc ctcaagggca tcgcggtgga caggattcgc gcgctgccgg
     1441 gcgtcgtggc ggtgatcacg gcggccgaca tccccggcgt caacgactgc ggcgccatcg
     1501 tccacgacga cccgatcctg gccgacggcc acgtgcacta cctcggccag cccgtcttcg
     1561 cggtgatcgc gaccgaccgc gagctcgcgc gccgcgcggc cgggcaggcc aggcagttcc
     1621 tggacatcga gccgctgccg gcgctcatca ccacggccga ggcgcacgcc gcgcagcagt
     1681 acgtgctgcc gccgatgcac ctggaacgcg gcgacgcgaa ggccgccatc gccgccgcgc
     1741 cgcgccacct gaaaggcacg atcagcctcg gcggccagga gcagttctac ctcgagggcc
     1801 agatctcgta cgcgatcccg accgagaacg acggcatgcg cgtgcactgc tcgacgcagc
     1861 atcccagcga gatgcagcac ctggtggcgc acgcactgcg cctcgactcg caccacgtgc
     1921 acgtcgaatg ccggcgcatg ggcggcggct tcggcggcaa ggagtcgcag tcgggcctct
     1981 tcgcgtgcat cgccgcgatc gccgcgcgcc agctcgatcg cccggtcaag atgcgtcccg
     2041 accgcgacga cgacttcatg atcaccggcc gccgccacgg tttcgagtac tcgtacgagg
     2101 tcggcttcga cgacgcgggc cgcgtgctgg gcgccgagat cgacatggtc tccaacgccg
     2161 gcttctcgac cgacctgtcg ggcccggtga tgacgcgcgc gctgtgccac ttcgacaacg
     2221 cctactggct gcccgacgtg gccatccacg gctacagcgc gcgcaccaac acgcagagca
     2281 acaccgcgtt ccgcggcttc ggcggcccgc agggcgcgtt cgcggccgag gtcatcctcg
     2341 actcgatcgc ccgcgcgctc ggcaaggacg cgctcgaggt gcggcgcgtc aacttctacg
     2401 gcatcggcga gcgcgacgtc acgccgtacg gccagcgcgt cgtcgacaac gtcatccgcc
     2461 cgctcaccga cgagctcgcc gccaaggcgg actacgcgca gcgccgggcc cggatcaacg
     2521 cgttcaatgc gacgagcccg gtgctcaaga agggcctggc gctgacgccg gtcaagttcg
     2581 gcatctcgtt caacgtcaag cacttcaacc aggccggcgc gctggtgcac gtctacgcgg
     2641 acggcacgat gctcgtgaac cacggcggca ccgagatggg ccagggcctg aacaccaagg
     2701 tggcgcaggt ggtggcgcac gagctgggcg tcagcttcga caccgtgcgc gccacggcca
     2761 ccgacacgac caaggtggcc aacacctcgg ccaccgcggc ctccaccggc gccgacctga
     2821 acggcaaggc cgcgcaggac gcggcgcgcc ggatccgcga gcggctcgcc gacttcgccg
     2881 cgcggcgcca cggcggcgac gccgcggacg tgcgcttcgc caacgacgtc tgccatgtca
     2941 acggccacgc gatcccgttc cccgagctcg cgcgcgccgc ctacgaggac cgcgtgcagc
     3001 tctggtcgga cggcttctac gcgacgcccg gcctgcactg ggatcgcgtc aagctgcagg
     3061 gccggccgtt cttctacttc gcgtacggcg ccgcgctgag cgaggtggtc gtcgacacgc
     3121 tgaccggcga gtggaagctg ttgcgcgccg acgtgctgca cgacgtcggc cgctcgctga
     3181 atccggcggt cgacatcggc caggtcgaag gcgccttcat ccagggcatg ggctggctca
     3241 cgatggagga gctggtctgg catccgaaga ccggcctgct cacgacgcac gcacccagca
     3301 cgtacaagat cccgaccgcc aacgattgcc cgccggtctt caacgtcgcg ctgtacgaca
     3361 acgccaacgt cgaggacagc atccaccgca gcaaggcggt cggcgagccg ccgctgctgc
     3421 tgccgttctc ggtgttcctg gcgatccgcg atgcggtctc ggcgctgggc gatcatcgcg
     3481 tcgacccgcc gctgcgcgcg ccggccaccg gcgaggccat catggacgcc gtcgacgcgg
     3541 tgcgccgcgg cctggccggc aaggaggcgt catgagcgcc gcccggccgc ccgaaggcgc
     3601 tcgcaccgca gtccgcaggg cggaggttgc ctcatgagcg ccgccctgca cgccaccgcc
     3661 gcgcgctggc tggcggccgg cgagccgggc gtcgtcatcg aagtcgtgaa gaccgcgggc
     3721 tcggtgccgc gcgagacggg cacgcgcatg ctggtctcgg ccacgcaggc ggtcggcacg
     3781 atcggcggcg gccacctcga gctgatggcc atcgggaacg cccgcacgct gctggcgcac
     3841 gttccggact tcgcgccggt cgatcgccac tatccgctcg gccccgcgct gggccagtgc
     3901 tgcggcggcg cggtcacgct gcgcttcgcg cggctcgacg cggcgaccgt gcgcgcctgg
     3961 ccgctcgtga cgccgcgctt cgagctgcag ctgtacggcg ccgggcacgt gggccgcgcg
     4021 atcgtgaagg cgctgtccgg catcgcctgc cgcgtgcaat ggatcgacga gcgcgaggac
     4081 gaatttcccg ccgacaccgc gctgccgccg cacatcgaga aggtgtgcgt cgatccggtg
     4141 gaagccgagg tggacgtcgc gcccgccaac agctgcttcc tcgtgctgac gcaccggcac
     4201 gacctggaca tgcgcatcgc cgaggccatc ctgcgccgcg gcgatttccg ctacctgggc
     4261 c
//
LOCUS       DATCRO010000080         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_754744, whole genome shotgun sequence.
ACCESSION   DATCRO010000080 DATCRO010000000
VERSION     DATCRO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754744"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VIN75_02140"
     CDS             <1..499
                     /locus_tag="VIN75_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8972987.1"
                     /translation="EWGLAKNENPNQGAFIIEELTELVEEAVLAEFEKIAERGGVLGA
                     METGYQRGKIQEESMHYEMLKHTGEYPIVGVNTFRNPNEAPLEHIELARSTDDEKQSQ
                     LTRLADFHAAHADEAPKMLAKLQAAVIANTNVFEVLMEAVRVCSLGQITGALFEVGGQ
                     YRRSM"
     gene            711..1394
                     /locus_tag="VIN75_02145"
     CDS             711..1394
                     /locus_tag="VIN75_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8972988.1"
                     /translation="MNLLLAEDDAILADALTEQLRHAGFETEHAPNGAVAEFLLLKQS
                     WDLCILDLGLPMVDGLTVLQRVRQRKPELPVLVLTALDDLQSRVAGLNAGADDYLTKP
                     FDFPELEARLRALLRRSQSSPVANASMAMGSLVFERDSRRASIDGAPLELSPREWTLL
                     DLLLTRRDRVVTKEEVAQAWSSERAEPGGNNSIEVYIHRVRRKLEGSGLSIRTVRGLG
                     YLLEALRPA"
     gene            1408..2847
                     /locus_tag="VIN75_02150"
     CDS             1408..2847
                     /locus_tag="VIN75_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8972989.1"
                     /translation="MKTLPSPLMLRLTRLVVGDQSLHKQLLLWLVLPQLVLWMAAAFV
                     TYNVAARYANRAIDASLLQATRALARQVKPIGNGLFIDFPRAADDIIGGDPDDRVYYE
                     VSTPPGKFILGSRTLPPPALTGPPKLGEPYFYDGVVDKMQIRVAALYVAYGDSKTRQL
                     MLVQIARGRARRDELARSILGDTVLPLSGLILLMTMIVWAGIRAGLAPLARMRALVED
                     RAPNDLAPLKIDAAPQELRSLARALNDLLAAVHESVAAQRRFVNDAAHQLRTPLAGLK
                     SQTELALGENVDPARQPRLQRVHESAVRSAHLVNQLLSLARAEPESALAQHSARVDLR
                     DLAREVTAEWVPKALQAGVDLGLDDAGEAAPPVVVQGHPLLIREALQNLIDNAIRYAG
                     AASEVTVCVRRDGSSGVLQVSDTGPGIPPDLHRAVFERFVRATHEGSGCGLGLAIVKS
                     IAERHGGQVSLTGIAPHGLQVTIALPLAR"
     gene            3262..>3480
                     /locus_tag="VIN75_02155"
     CDS             3262..>3480
                     /locus_tag="VIN75_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY8972990.1"
                     /translation="MQDTRGSRPAPRLVAVAAFALLAGNAAHAVDWTGYMRGGPAATT
                     VSGESRQCYGLNSPGLKYRLGNECDFYGE"
BASE COUNT          534 a         1231 c         1201 g          514 t
ORIGIN      
        1 cgagtggggc ctggccaaga acgagaaccc gaaccagggc gcgttcatca tcgaggagct
       61 caccgagctg gtcgaggagg cggtgctggc cgagttcgag aagatcgccg agcgcggcgg
      121 cgtcctgggc gcgatggaga ccggctacca gcgcggcaag atccaggaag agtcgatgca
      181 ctacgagatg ctcaagcaca cgggcgagta cccgatcgtg ggcgtgaaca ccttccgcaa
      241 cccgaacgag gcgccgctgg agcacatcga gctggcgcga tccaccgacg acgagaagca
      301 gagccagctc acgcgcctgg ccgacttcca cgcggcccac gccgacgagg cgccgaagat
      361 gctcgcgaag ctgcaggccg cggtgatcgc caacaccaac gtgttcgagg tgctgatgga
      421 ggcggtgcgg gtctgctcgc tgggacagat caccggggcg ctgttcgagg tgggtggcca
      481 gtaccggcgc agcatgtaaa tggagcccca cggtcgctcg cgctccctgc ccccgggggg
      541 gcggccgcga ctggcccggc agagccggat ccagcgctcc ctcgaacgct gatcggggca
      601 ctcgagcggc agcgccgggc tgaatgcgcg ctgcgcgcat tggcgttttc gcgacgttcg
      661 cgcgtattca tcgatctgcc cgcggccacg gccgggttaa gcttccgatc atgaacctcc
      721 tgctcgccga agacgacgcg atcctggccg atgcgctcac ggagcagttg cgccatgccg
      781 gcttcgagac ggagcacgcg cccaacggcg ccgtcgccga gttcctgctg ctcaagcagt
      841 cgtgggacct gtgcatcctc gatctgggcc tgccgatggt ggacggcctg acggtgctgc
      901 agcgcgtgcg ccagcgcaag ccggagttgc ccgtgctggt gctgaccgcg ctggacgacc
      961 tgcagagccg cgtcgcgggc ctgaacgccg gcgcggacga ctacctcacc aagccgttcg
     1021 acttccccga gctggaggcg cgcctgcgcg cgctgctgcg gcgcagccag tcgtcgccgg
     1081 tggccaacgc ctcgatggcg atgggctcgc tcgtgttcga gcgcgactcg cgccgtgcga
     1141 gcatcgacgg cgcgccgctg gagctcagcc cgcgcgaatg gacgctgctg gacctgctgc
     1201 tcacgcgccg cgaccgcgtc gtcaccaagg aagaagtggc gcaggcctgg agcagcgagc
     1261 gcgcggagcc cggcggcaac aattcgatcg aggtctacat ccaccgcgtg cgccgcaagc
     1321 tggaggggtc gggcctgagc atccgcacgg tgcgcggtct cggctacctg ctcgaggcgc
     1381 ttcgccccgc ctgatcgcgc gctccgcatg aagaccctgc ccagcccgct gatgctgcgg
     1441 ctgacgcggc tggtcgtcgg cgaccagtcg ctgcacaagc agctgctgct gtggctggtg
     1501 ctgccgcagc tggtgctctg gatggcggcc gccttcgtca cctacaacgt cgcggcgcgc
     1561 tacgcgaacc gcgccatcga tgccagcctg ctgcaggcca cgcgcgcgct ggcgcgccag
     1621 gtcaagccga tcggcaacgg cctgttcatc gacttcccgc gcgcggccga cgacatcatc
     1681 ggcggcgacc ccgacgaccg cgtgtactac gaggtcagca cgccgccggg caagttcatc
     1741 ctgggcagcc gcacgctgcc cccgccggcg ctcaccggtc cgcccaagct gggcgagccc
     1801 tacttctacg acggcgtcgt cgacaagatg cagatccgcg tcgcggcgct gtacgtggcc
     1861 tacggcgaca gcaagacgcg ccagctgatg ctcgtgcaga tcgcgcgcgg ccgcgcgcgc
     1921 cgcgacgagc tggcgcgcag catcctgggc gacacggtgc tgccgctctc gggcctgatc
     1981 ctgctgatga cgatgatcgt gtgggccggc atccgcgccg gcctcgcgcc gctggcgcgc
     2041 atgcgggcgc tggtggagga tcgcgcgccc aacgacctcg cgccgctgaa gatcgacgcc
     2101 gcgccgcagg agctgcgctc gctggcgcgc gcgctcaacg acctgctggc cgccgtgcac
     2161 gagagcgtgg ccgcgcagcg ccgcttcgtc aacgacgcgg cgcaccagtt gcgcacgccg
     2221 ctggccggcc tcaagagcca gacggaactg gccctcggcg agaacgtcga cccggcgcga
     2281 cagccgcggc tgcagcgcgt gcacgagagc gcggtgcgca gcgcccacct ggtcaaccag
     2341 ctgctgagcc tggcgcgtgc cgagcccgaa tcggcgctcg cgcagcacag cgcgcgcgtc
     2401 gacctgcgcg acctcgcgcg cgaagtcacg gccgagtggg tgcccaaggc cctgcaggcc
     2461 ggcgtggacc tgggcctgga cgacgccggc gaggcggcgc cgccggtggt cgtgcaggga
     2521 cacccgctgc tcatccgtga ggcgctgcag aacctgatcg acaacgcgat ccgctacgcc
     2581 ggtgcggcca gcgaggtgac ggtttgcgtg cggcgcgacg gcagcagcgg cgtgctgcag
     2641 gtctccgata caggaccggg tatcccgccc gacctgcacc gtgccgtgtt cgagcgcttc
     2701 gtgcgcgcca cgcacgaggg ttcgggctgc ggcctgggcc tggcgatcgt caagagcatc
     2761 gccgagcgcc acggcggcca ggtctcgctg accggcatcg cgccgcacgg cctgcaggtg
     2821 acgattgcgc tgccgctggc gcgctgaagg cagcgctccc aggctgcgcc gggcgcgcgc
     2881 gccgctggtt cgggaccagc ccttgttttt acgtcgattc gcggtcgccg tcctgcgtgg
     2941 tagcgatctc ttacggcctc cttcggcact tcggcaacag atttgcgttc aggtcgcgtc
     3001 aatctagggg cattgcttcc ggaggccggt ccagccttgt acggtcgtgc tccagctcga
     3061 tcggcccgtc gccagtgcgt ttcgtgcaca aatgcgcatg ccctgctcat ctgttgcaag
     3121 gactcgacaa tctgctcgac actacgcgaa aaccctcatt aattttgcat taatggctga
     3181 ttaagcttct taacgttggc ccgcgccccc gtgcggctag cgaaccacaa cgcactcccg
     3241 agtgcaaagc aggagacaga catgcaagac acccgaggct cgcgcccggc accccgactc
     3301 gttgccgttg ccgccttcgc ccttctcgcc ggcaatgcgg cccacgcggt cgactggacc
     3361 ggctacatgc gcggcggccc cgcggccacc acggtctctg gcgaatcgcg ccagtgctac
     3421 ggcctgaaca gcccgggcct gaagtaccgc ctgggcaacg agtgcgactt ctacggcgag
//
LOCUS       DATCRO010000081        11564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_203583, whole genome shotgun sequence.
ACCESSION   DATCRO010000081 DATCRO010000000
VERSION     DATCRO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203583"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIN75_02160"
     CDS             <1..324
                     /locus_tag="VIN75_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY8972991.1"
                     /translation="ANKAIEEVRQAGGVGSSLQAELTIAANAEDRALLASLGDDLKFV
                     TITSAVTVVEGDELVVTVTPSTSPKCERCWHWRADVGADAAHPTICGRCTSNLFGTGE
                     VRSVA"
     gene            complement(478..1143)
                     /locus_tag="VIN75_02165"
     CDS             complement(478..1143)
                     /locus_tag="VIN75_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8972992.1"
                     /translation="MSFALSQRRTDRHPVGLLIVVGLHVVLAAALLSAKLQHPPAREV
                     AIPMQPLDEPAKPTPPKPVDLPPPPHVLPHTIVVVPQDFPTDPPPPDAPRGVPDDGQK
                     PQPVVVASIGPDKPPVSVPHIQARPARLDAGAAQCRPDYPAAAQRAGATGSTRIRFTV
                     DALGRIAGSQILHASGPTREHRLMDQAAAAALAQCPVQVGTDEQGRPVGTTTDVEYVW
                     SLN"
     gene            1454..1963
                     /gene="lspA"
                     /locus_tag="VIN75_02170"
     CDS             1454..1963
                     /gene="lspA"
                     /locus_tag="VIN75_02170"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8972993.1"
                     /translation="MATRSLSRSTGSSPSMWLWLGLALAVVLLDQFAKALIVADMRLG
                     ESRTVTSFFDVVRFENTGMAWSLLATAGGWQRWFFIGLALAASAFMAYLLRRNGGQKL
                     FSFAIAMVMGGALGNVVDRIARGAVVDFISLHWHQAYTFPAFNLADSAITLGAICLLV
                     DELLRVRRG"
     gene            1969..2793
                     /locus_tag="VIN75_02175"
     CDS             1969..2793
                     /locus_tag="VIN75_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY8972994.1"
                     /translation="MRHACPHRLASAAALLLALCTPAARAALPAGLAANDLEAMTSVE
                     LRERIAHGATTIIVPIGGTEQNGAHMLLGKHNVRAHVLADRIADALGNAIVAPTIAYV
                     PEGSIHPPAAHMKYAGTISVPDAVFEGLLEATARSFKQHGFRDVVFLGDHGGYQSDLT
                     QVADKLNREWARDPACRAHAPLDYYRITQTAYVAALKSHGVSEAELGSHAGLADTALA
                     LAVDPALVRTDRLAGPYPAGVTGNPRRATAELGELGTRLVVETTVAAIRKETQAPR"
     gene            2816..3754
                     /locus_tag="VIN75_02180"
     CDS             2816..3754
                     /locus_tag="VIN75_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8972995.1"
                     /translation="MPTAVDYPMLPLLDMTLFPGSETSLFVGRALSRASVEAAVAGNK
                     ELVVVRQREFGTLEFTATDLHTVGTLATVTSLTRLPDGTLKITVTAHGRVSIDSVRRS
                     DHFRVFATVLPTPPCPPARSARLRGALLRRVGTALSPEQLALLRQVPEAGKLVDAIMP
                     MLATPSARSIHPHHSLHQGLLEKLDGEQRLLKLMKMLPHNRRAEVPANGEFTQALPLP
                     GFDPAGEPQARTTADGSLWLVFGAMPPTWYVPNDATGPLGPCTDLDRLLERATGCAIF
                     WDSLKLLQIDNPRADTVARIERFLRSVRQNWAAEAN"
     gene            3891..4538
                     /locus_tag="VIN75_02185"
     CDS             3891..4538
                     /locus_tag="VIN75_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972996.1"
                     /translation="MSIESSLPAWHRHAIVPLALSGGLTLVMLHGAPGVLLGVALLGA
                     SLSALLADRLRHHVRMQAQFCEQWSHVVAPVQAGQATPGPSQPAVDTSDLDRRRRGVS
                     TKAAALRLAMAGGEEAAELELQLRDLMDALSRHVRRETDLLARQADARRRADVDAARL
                     QLANAEYDFHRYCIGELTVGELVDRVTTALISGQAPMSDLPGNDRAPSRMGEIDA"
     gene            4683..5939
                     /locus_tag="VIN75_02190"
     CDS             4683..5939
                     /locus_tag="VIN75_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY8972997.1"
                     /translation="MNATSRSALSLLALAALAALAAVGHAADPAASGTAHAVVTVPGM
                     PPVVDPSNLYSETAGPEHIKPELKDDLVRVYVPNLRGNSVSVIDPAKMKVVDTLKVGQ
                     SPQHVVVGYDLRTLWAANNAERSNNGSLTPIDPRTGKTGAAIPVDDPYNMYFTPDGKS
                     AIVVAEARHRLDFRDPKTMAMQYSIDAPRCGGINHADFSIDGRTAFFTCEFEGSIAKI
                     DLVDRKVAGYLVLPMPATRLKDKFVPAGGPTTEVCTSRKGMPQDIRFSPDGKKLYVAD
                     MEADGVHVLDAAAFKETAFITTGVAAHGLYPSRDGRHLYVANRGSHKIHGPRKGPGSV
                     AVLDFATDKIIANWAVPGGGSPDMGNVTADGKYLWLSGRYDDVVYRFDTATGAVQQVA
                     VGQEPHGLTVWPQPGRYSLGHTGNLR"
     gene            6054..6584
                     /locus_tag="VIN75_02195"
     CDS             6054..6584
                     /locus_tag="VIN75_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8972998.1"
                     /translation="MTKTSFIARVAAGAAALVVSATASAEGTFAPELGIGWGGGKEVG
                     IQGDRQSMFGTAAFGYTADNGLGGRLILIADMNIVRGAFFTQERSFDNFIGVQGTMAV
                     PLAERLKFVGGLGIGRTHLDDGSGGASDSAIITDGLVSAGLRYRFANHYAMEALVSHL
                     TTSDLTTATLQFQVPF"
     gene            6584..7048
                     /locus_tag="VIN75_02200"
     CDS             6584..7048
                     /locus_tag="VIN75_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8972999.1"
                     /translation="MTTASLPSLRPAVEADLPFLVELRLATMAPHFARQGLVVSVGEH
                     CRRARYRLDAATIIELDGHPVGVIKVIQDTRTWTIEQFQIVPAHQGRGLGETVLRALI
                     AEARHAGALLHLSVLKQNPAARLYARLGFRTLLESDQSYKMTFIETEAPDGV"
     gene            complement(7058..8041)
                     /locus_tag="VIN75_02205"
     CDS             complement(7058..8041)
                     /locus_tag="VIN75_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY8973000.1"
                     /translation="MSKAIQISEFGGPEVLRLVDVTVGDPGPGQVRVRHHACGINFID
                     VYQRTGLYPSPLPLIPGGEGAGVVEAVGEGVAHLAVGDRVAYAAPKPGSYCEVRVLDA
                     LHVCRLPDDIDFETGAAMMLKGLTVEYLLRRTLPVEGLAPGDCILFHAAAGGVGLIAC
                     QWAKALGLSLIGTAGSDDKCALAREYGAAHMINYRSENFAERVKEITGGKGVKVVYDS
                     VGKDTFDRSLDCLRPFGLMVVFGNASGPAAPVAPNTLAAKGSLYLTRPTLFTHLATRE
                     RTQAMADELFEVVRDGTVKVRIDQRYPLAEAAQAHRDLEARKTTGCSVLLA"
     gene            complement(8122..9729)
                     /locus_tag="VIN75_02210"
     CDS             complement(8122..9729)
                     /locus_tag="VIN75_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY8973001.1"
                     /translation="MNFDWHNPYATVRSPVFARNVVSTSHPLASQAGLRMLLKGGNAI
                     DAAIAAAAALTIVEPVSCGLGSDLFAILWDGQRLLGLNSSGVAPAAWDRAYFDRRHGG
                     AMPVRGWDSVTVPGAVAGWVALSERCGRLPFADLLEPAIEQAERGHGVACIVADKWAR
                     QVPLLQAQPGFAEAFMPHGRAPQPGERFVFEAAGRTLRAIADTRGESFYRGEIAAALV
                     AHAAANGGSMTAADLAGWLPDWVEPISITFAGHTVHQIPPNGQGIAALMALGMLEQLD
                     VVRFPVDSIESQHLQIEAMKLAFADTYRWVADHRSMTEVTAAHLLSPAYLRERARLIV
                     PGLAQDFVHGTPPPGGTVYLTAADEQGRMVSLIQSNYMGFGSGVVVPGTGVSLQNRGQ
                     GFTLDAAHPNVVAPGKRPFHTIIPGFLSQDGRPRMSFGVMGGNMQPQGHVQTLMRMLR
                     HGQQPQAACDAPRWKVATGLAIDFERTMAPGLVAGLHALGHQSEATADAYMDFGSGQF
                     IWRLSDDPADGYVAASDSRRDGQAVGF"
     gene            complement(9755..10708)
                     /locus_tag="VIN75_02215"
     CDS             complement(9755..10708)
                     /locus_tag="VIN75_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973002.1"
                     /translation="MASESTTTPTLASLSTTTPVPRPQRLWPGLALATAGSIAFSGKA
                     IIVKLAYRYGVDAVTLIMYRMLFALPLFAAIAWWAGRGKPGLAARDWRVVAGLGFSGY
                     YLASFLDFAGLAYISAGLERLVLYLNPTLVLLIGRVLFKRRVERSQLLALAVSYAGVL
                     LVFGHEAHVGGGHVALGVALVFGSAISYATYMVFSGEEVRRLGSLRLTGLASTVACGL
                     CLLQFVLLRPLSSAAVAPQVLWLSMLNATACTVAPVLMVMMAIERIGATVAAQTGMVG
                     PLSTILMGIVILGEPFTIWIAAGTVFVLSGIWLLAKSKPKT"
BASE COUNT         1715 a         4231 c         3988 g         1630 t
ORIGIN      
        1 gccaacaagg cgatcgagga agtgcgccag gccggcggcg tcggctcctc gctgcaggcc
       61 gagctgacga tcgccgccaa cgcggaggac cgcgcgctgc tggcctcgct gggcgacgac
      121 ctgaagttcg tcaccatcac gtcggccgtg acggtggtcg agggcgacga gctcgtcgtg
      181 acggtgacgc cgtcgacgtc gcccaagtgc gagcgctgct ggcactggcg cgcggacgtc
      241 ggcgcggacg ccgcgcatcc gacgatctgc gggcgctgca cgagcaacct gttcggcacg
      301 ggcgaagtgc gatcggtggc ctgatcggtc gttcccgttt ccttcgacaa ggcggcgccg
      361 cgaggcgccg cctcgtttgc tttccggcga cgaatggcgg gttacggcaa gagatagagc
      421 ggaaccgtag ggcgggtcag ccgaaggcgt aacccgccat ccgccgtccc gctgcgatca
      481 attcaacgac cagacgtact cgacgtcggt ggtcgtcccg accggccggc cctgctcgtc
      541 ggtgccgacc tgcaccgggc actgcgccag cgcggcggcc gcggcctggt ccatcaggcg
      601 gtgctcgcgc gtcggccccg aggcatgcag gatctgcgaa ccggcgatgc gcccgagggc
      661 atccacggtg aagcggatgc gcgtgctgcc cgtcgcgccg gcgcgctggg ccgccgcggg
      721 atagtcgggc cggcactgcg cggcgccggc gtccagccgc gccggccggg cctggatgtg
      781 cgggacgctc acgggcggct tgtccgggcc gatcgacgcg accacgacgg gctgcggctt
      841 ctgcccgtcg tccggcacgc cccgcggggc gtcgggcggc ggcggatcgg tcgggaagtc
      901 ctgcggcacc acgacgatgg tgtgcggcag cacgtgcggc ggcggcggca ggtcgaccgg
      961 cttgggcggc gtcggcttgg ccggctcgtc caggggttgc atcggaatgg ccacttctct
     1021 ggccggcggg tgctgcagct tggcggacag cagggccgcg gccagcacga catgcaggcc
     1081 gacgacgatc aacaggccga ccggatggcg gtcggtgcga cgctgcgaaa gagcaaagct
     1141 catgcggatc tcctccagtt gggccacggg gcgcgccggc cggcccggcc ggatcgcgcg
     1201 ttcgtctcga tgaaacgggc cagcggccga aagagggtga acgaacgccg gattcttccc
     1261 ggccgtggcc acgatgccgc ggcgcccgct tccgggggtt gctcgcacgc tggcggtcaa
     1321 tgggggcacg ccccttgttt cgtacgtttg ctaacagtca ggaagcgctc gacaacggct
     1381 aaagtaggtg cttttgcgcg gagtcaatcc cccgcgcgaa ggcatcgcgc ctccagcccc
     1441 ctttttgtcc ctgatggcca cccgttccct ttcccgctcc accggctcca gcccttcgat
     1501 gtggctctgg ctcggcctcg cgctcgccgt ggtcctgctc gaccaattcg ccaaggcgct
     1561 gatcgtcgcc gacatgcgcc tcggcgagag ccgcaccgtc acctccttct tcgacgtcgt
     1621 ccggttcgag aacaccggca tggcctggtc gctgctggcc accgccggcg gctggcagcg
     1681 ctggttcttc atcgggctgg cgctcgccgc ctcggccttc atggcgtacc tgctgcgccg
     1741 caacggcggg cagaagctgt tctcgttcgc catcgcgatg gtgatgggcg gcgccctcgg
     1801 caacgtcgtc gaccgcatcg cgcgcggcgc ggtggtcgac ttcatctcgc tgcactggca
     1861 ccaggcctat accttcccgg cgttcaacct ggccgacagc gccatcacgc tgggcgccat
     1921 ctgcctgctc gtcgacgagc tgctgcgcgt gcgccgggga tgaccgcctt gcgacacgct
     1981 tgcccacacc ggctcgccag cgccgccgcc ctgctgctgg cgctgtgcac gcccgcggcg
     2041 cgcgccgcgc tgcccgccgg cctggccgcc aacgacctcg aggcgatgac cagcgtcgag
     2101 ctgcgcgagc gcatcgcgca cggcgccacg acgatcatcg tgcccatcgg cggcaccgag
     2161 cagaacggcg cccacatgct gctgggcaag cacaacgtgc gtgcccacgt gctggccgac
     2221 cgcatcgccg acgcgctcgg caacgccatc gtcgccccca cgatcgccta cgtgcccgag
     2281 ggcagcatcc atccgccggc cgcgcacatg aagtacgccg gcacgatctc ggtgcccgat
     2341 gccgtcttcg aaggcctgct cgaggccacc gcccggtcgt tcaagcagca cggcttcagg
     2401 gacgtcgtgt tcctgggcga ccacggcggc taccagtccg acctgacgca ggtggccgac
     2461 aagctcaatc gcgagtgggc cagggatccg gcctgccgcg cccacgcgcc actggactac
     2521 taccgcatca cgcagaccgc ctacgtcgcc gcgctgaagt cccacggcgt gtcggaggcc
     2581 gagctgggct cgcacgccgg actggcggat actgcactgg cgctcgcggt cgatcccgcg
     2641 ctcgtgcgca ccgacaggct ggccggcccc taccccgcgg gcgtcaccgg caacccgaga
     2701 cgcgccaccg ccgaactggg cgagctgggc acccgcctgg tcgtcgagac caccgtcgcc
     2761 gcgatccgca aggaaacgca ggccccccgt tgagaaacgc cgcacgttga cccccatgcc
     2821 gaccgccgtc gactatccga tgctgccgct gctggacatg acgctcttcc cggggagcga
     2881 gacgtcgctg ttcgtgggac gggcgttgtc gcgcgcgtcg gtggaggcgg ccgtcgcggg
     2941 caacaaggag ctcgtggtgg tgcgccagcg cgagttcggc acgctcgaat tcaccgccac
     3001 cgacctgcac acggtgggca cgctcgccac cgtcacgtcg ctcacgcggc tgcccgacgg
     3061 cacgctcaag atcaccgtca ccgcgcacgg ccgcgtgtcg atcgactccg tccggcgctc
     3121 cgaccacttc cgcgtcttcg ccaccgtgct gcccaccccg ccgtgtccgc ccgcgcgctc
     3181 ggcgcggctg cgcggggcgc tgctgcggcg cgtcggcacc gccttgtcgc ccgagcagct
     3241 ggccctgctg cgccaggtgc ccgaggccgg caagctggtc gatgccatca tgcccatgct
     3301 ggccacgccc agcgcgcgca gcatccatcc gcaccactcg ctgcaccagg ggctgctcga
     3361 gaagctcgac ggcgagcagc gcctgctcaa gctgatgaag atgctgccgc acaaccggcg
     3421 cgccgaggtg ccggccaacg gcgagttcac ccaggcgctg ccgctgcccg gcttcgaccc
     3481 cgcgggcgag ccgcaggcgc gcacgacggc cgacggcagc ctgtggctcg tgttcggcgc
     3541 gatgccgccg acctggtacg tcccgaacga cgccaccggc cccctgggcc catgcacgga
     3601 cctcgaccgg ctgctggagc gcgccaccgg ctgcgcgatc ttctgggaca gcctgaagct
     3661 gctgcagatc gacaacccgc gggccgacac ggtggcccgc atcgagcgct tcctgcgcag
     3721 cgtccgccag aactgggccg ccgaagccaa ctgacggccc cggccgcgac tttgttcacg
     3781 ccaggccgcg tcgcacccgg ggggccaccc tccaccaccg cgcggccggc ttcaatatgg
     3841 cgacaggcag gccgcatcga gcctgcatcg tcgtcgggaa gggggagtcc atgtccatcg
     3901 aatcgagcct gcccgcgtgg catcggcacg ccatcgtgcc gctcgcactg tccggcggcc
     3961 tcaccctcgt catgctgcac ggcgcgcccg gcgtgctgct gggcgtggcg ttgctgggcg
     4021 cgtcgctgag cgcgctgctg gccgaccgcc tgcgccacca cgtgagaatg caggcgcagt
     4081 tctgcgagca gtggtcccat gtcgtggcgc cagtgcaggc cgggcaggcc acacccgggc
     4141 cgtcgcagcc cgccgtcgac accagcgacc tcgaccgccg ccgccgcggc gtctcgacca
     4201 aagccgccgc gctgcgcctg gcgatggccg gcggcgagga ggccgccgag ctcgagctcc
     4261 agctgcgcga cctgatggac gcgctctcgc gccacgtgcg gcgcgagacc gacctgctcg
     4321 cccgtcaggc cgacgcgcgc cgacgcgccg acgtcgacgc cgcgcgcctg cagctggcca
     4381 acgcggaata cgacttccac cgctactgca tcggcgagct gacggtgggc gaactcgtgg
     4441 atcgcgtgac cacggcgctg atctcgggcc aggcaccgat gtccgacctg ccgggcaacg
     4501 accgggcccc gagccgcatg ggcgagattg acgcgtagca acgacccgtc attcccgggc
     4561 cacggccggg caacggcagc tcatttcccg gcgagaatcg aggacgacgc gaggcggcgc
     4621 gcgccaggga acgttcccgg ccgcggcgcc tcgaacccgt tccccgacac ccgaactcgc
     4681 ccatgaacgc cacctcccgc agcgcgctgt cgctgctcgc gctcgcggcc ctggcggcgc
     4741 ttgccgccgt cggccacgcc gccgaccctg ccgcgtccgg caccgcccac gccgtcgtca
     4801 ccgtgcccgg catgccgccg gtcgtcgacc ccagcaacct gtacagcgag accgccggcc
     4861 ccgagcacat caagcccgag ctcaaggacg acctggtgcg cgtgtacgtg cccaacctgc
     4921 gcggcaactc ggtcagcgtg atcgaccccg cgaagatgaa ggtcgtggac acgctcaagg
     4981 tcggccagag cccgcagcac gtcgtcgtgg gctacgacct gcgcacgctg tgggcggcca
     5041 acaatgccga acgcagcaac aacggcagcc tcacgccgat cgacccgcgc accggcaaga
     5101 ccggcgcggc catcccggtc gacgacccgt acaacatgta cttcacgccg gacggcaagt
     5161 cggccatcgt cgtcgccgag gcgcgccacc gcctcgactt ccgcgacccg aagacgatgg
     5221 cgatgcagta ctcgatcgac gcccccaggt gcggcggcat caaccacgcc gacttctcca
     5281 tcgacggccg caccgcgttc ttcacctgcg agttcgaggg ctccatcgcc aagatcgacc
     5341 tggtcgaccg caaggtggcc ggctacctcg tgctgccgat gcccgccacg cgcctgaagg
     5401 acaagttcgt gccggccggc ggccccacca cggaggtctg cacctcgcgc aagggcatgc
     5461 ctcaggacat ccgcttctcg cccgacggca agaagctgta cgtggccgac atggaggccg
     5521 acggcgtcca cgtgctcgac gccgccgcgt tcaaggagac ggccttcatc accaccggcg
     5581 tcgccgcgca cggcctgtac cccagccgcg acggcaggca cctgtacgtg gccaaccgcg
     5641 gctcgcacaa gatccacggc ccgcgcaagg gcccgggcag cgtggccgtg ctggatttcg
     5701 ccaccgacaa gatcatcgcc aactgggcgg tgcccggcgg cggcagcccc gacatgggca
     5761 acgtcacggc cgacggcaag tacctgtggc tctcgggccg ctacgacgac gtcgtctacc
     5821 gcttcgacac cgccaccggc gcggtgcagc aggtcgccgt cggccaggag ccgcacggcc
     5881 tgaccgtgtg gccgcagccg ggccgctact cgctggggca taccggcaac ctgcgctgag
     5941 ctatgctcgg cactgcctga gctctgcctg acagggcaag gccgcatgaa ggacgcgccc
     6001 cgcgagggcg cgttcgcatg tcttcgccat ccaaattcct ggggaggaat ccaatgacca
     6061 agacttcgtt catcgcgagg gtcgccgcgg gcgcggctgc gcttgtcgtc tctgccaccg
     6121 cgtccgcgga aggcacgttc gcgcccgaac tgggcatcgg ctggggcggc ggcaaggagg
     6181 tggggatcca gggcgatcgc cagtccatgt tcggcacggc cgcgttcggc tacaccgcgg
     6241 acaacggcct cggcggccgg ctcatcctga tcgccgacat gaacatcgtg cgcggcgcgt
     6301 tcttcacgca ggagcgctcg ttcgacaact tcatcggcgt gcaaggcacc atggccgtcc
     6361 cgctggccga gcggctcaag ttcgtcggcg gcctcggcat cgggcgcacc cacctggacg
     6421 atggcagcgg cggcgcgagc gattcggcca tcatcaccga cggcctggtc agcgcgggcc
     6481 ttcgctatcg cttcgcgaac cactacgcga tggaggcgct cgtgagccac ctgacgacga
     6541 gcgacctcac gacggccacg ctgcaattcc aggtcccgtt ctgatgacca cggcctcgct
     6601 cccgagcctt cggccggcgg tcgaggccga cctgcccttc ctcgtggaac tgcggctggc
     6661 caccatggcc ccgcacttcg cgcgccaggg cctggtcgtg agcgtgggcg agcactgcag
     6721 gcgcgccagg tacaggctcg acgcggccac catcatcgag ctcgacgggc atccggtggg
     6781 cgtcatcaag gtgatccagg acacgcgcac ctggacgatc gagcagttcc agatcgtccc
     6841 cgcgcaccag ggccgcggcc tgggcgagac ggtgctgcgc gcgctgatcg ccgaggcgcg
     6901 ccacgccggc gcgctgctgc acctgagcgt gctcaagcag aacccggcgg cgcggctcta
     6961 tgcgcggctg ggctttcgta cgctgctcga atcggatcag tcgtacaaga tgaccttcat
     7021 cgagaccgag gcgccggacg gcgtctgacg cgccgcgtca ggcgagcagc acgctgcagc
     7081 cggtggtctt gcgcgcctcc aggtcgcggt gcgcctgcgc ggcctccgca agcgggtagc
     7141 gctggtcgat gcggaccttg acggtgccgt cgcgcaccac ctcgaacagc tcgtcggcca
     7201 tcgcctgcgt acgctcgcgc gtggccaggt gcgtgaacag cgtcgggcgc gtcaggtaca
     7261 gcgagccctt ggcggccagc gtgttcggcg cgaccggggc ggcggggccc gaggcgttgc
     7321 cgaacaccac catcagcccg aacggccgca ggcagtccag ggagcggtcg aaggtgtcct
     7381 tgcccaccga gtcgtagacc accttcacgc ccttgccgcc ggtgatctcc ttgacgcgct
     7441 cggcgaagtt ctcgctgcgg tagttgatca tgtgagcggc accgtattcg cgggccagcg
     7501 cgcacttgtc gtcggagccg gcggtgccga tcagcgacag tcccagcgcc ttggcccact
     7561 ggcaggcaat caggcccacg ccgccggcgg ccgcgtggaa caggatgcag tcacctggcg
     7621 cgaggccttc gaccggcagc gtgcggcgca gcaggtattc gacggtcagg cccttcaaca
     7681 tcatcgcggc gccggtctcg aagtcgatgt cgtcaggcag gcggcacacg tgcagcgcgt
     7741 cgagcacgcg cacctcgcag tagctgcccg gcttgggcgc ggcgtaggcg acgcggtcgc
     7801 cgacggccag gtgcgccacg ccctcgccca ccgcctcgac cacgccggcg ccctcgccgc
     7861 ccgggatcag cggcagcggg ctcgggtaca ggccggtgcg ctggtagacg tcgatgaagt
     7921 tgatgccgca cgcgtggtgg cgcacgcgca cctgcccggg gccggggtcg cccaccgtga
     7981 cgtcgacgag gcgcagcacc tcgggcccgc cgaattcact gatctggatc gccttgctca
     8041 cgggggtcct cctgctcgtc gatgtgggac ggcctgttgt agcacgcgag ccttgacctt
     8101 ggcgccccgt cggacggttg ctcagaaccc cacggcctgg ccgtcgcggc gcgagtcgct
     8161 ggcggcgacg tagccgtcgg ccggatcgtc cgacagccgc cagatgaact ggcccgagcc
     8221 gaaatccatg taggcatcgg ccgtggcctc ggactggtga cccagcgcgt gcaggcccgc
     8281 gaccaggccc ggcgccatcg tgcgctcgaa gtcgatggcc agcccggtgg ccaccttcca
     8341 gcgcggcgca tcgcacgcgg cctgcggttg ctggccgtgg cgcagcatgc gcatcagcgt
     8401 ctgcacgtgg ccctgcggct gcatgttgcc gcccatcacg ccgaagctca tccgcggccg
     8461 gccgtcctgg ctcaggaagc cggggatgat ggtgtggaac ggccgcttgc ccggcgccac
     8521 gacgttcggg tgcgcggcgt ccagcgtgaa gccctggccg cggttctgca ggctgacgcc
     8581 ggtgccgggc accacgacgc cgctgccgaa gcccatgtag ttggactgga tcaggctgac
     8641 catgcggccc tgctcgtcgg ccgccgtcag gtagacggtg ccgcccggcg gcggcgtgcc
     8701 atggacgaag tcctgcgcga gcccgggcac gatcaggcgc gcacgctcgc gcaggtacgc
     8761 gggcgagagc aggtgcgcgg cggtgacctc ggtcatggac cggtggtcgg ccacccagcg
     8821 gtaggtgtcg gcgaaggcga gtttcatcgc ctcgatctgc aggtgctgcg actcgatgct
     8881 gtcgaccggg aatcgcacga cgtccagttg ctccagcatg cccagcgcca tcagcgccgc
     8941 gatgccctgg ccgttgggcg ggatctggtg caccgtgtgg ccggcgaagg tgatcgagat
     9001 cggctcgacc cagtcgggca gccagcctgc caggtcggcg gcggtcatcg agccgccgtt
     9061 ggccgccgcg tgcgcgacga gcgcggccgc gatctcgccg cggtagaacg actcgccgcg
     9121 cgtgtccgcg atggcccgca acgtgcgtcc cgcggcctcg aacacgaagc gctcgcccgg
     9181 ctgcggcgca cgtccatgcg gcatgaaggc ttcggcaaag ccgggctgcg cctgcagcag
     9241 cggcacctgg cgcgcccact tgtcagccac gatgcaggcc acgccatggc cgcgctcggc
     9301 ctgctcgatg gccggctcca gcaggtcggc gaatggcagc cggccgcagc gttcggacag
     9361 cgccacccag cccgccaccg cgccggggac ggtcaccgaa tcccagccgc gcaccggcat
     9421 cgcgccgccg tggcggcggt cgaagtacgc gcggtcccac gcggccggcg cgacgcccga
     9481 cgagttgagc ccgagcagcc gctgcccgtc ccacaggatg gcgaacaggt cgctgcccag
     9541 gccgcacgac acgggctcga cgatggtcag tgccgccgcc gccgcgatcg ccgcgtcgat
     9601 cgcgttgccg cccttgagca gcatgcgcag cccggcctgc gaggccagcg gatgggaggt
     9661 cgagaccacg ttgcgcgcga acacggggct gcgcacggtg gcgtaggggt tgtgccagtc
     9721 gaagttcatg ggggcctttc cgcaggcccc attctcaggt cttcggcttg gacttcgcca
     9781 gcagccagat gcccgacagc acgaagacgg tgccggccgc gatccatatc gtgaacggct
     9841 cgcccaggat cacgatgccc atcaggatgg tgctcagcgg gccgaccatg cccgtctgcg
     9901 cggccaccgt ggcgccgatg cgttcgatgg ccatcatcac catgagcacc ggggcgacgg
     9961 tgcaggccgt ggcgttgagc atcgacagcc acagcacctg cggcgcgacg gcggccgacg
    10021 acagcggccg cagcagcacg aactgcagca ggcacagccc gcaggccacc gtgctggcca
    10081 ggcccgtcag ccgcagcgag cccaggcgcc tgacctcctc gccactgaac accatgtagg
    10141 tggcgtagct gatcgcgctg ccgaacacca gcgccacgcc gagcgcgacg tgaccgccgc
    10201 ccacgtgcgc ctcgtggccg aacaccagca gcaccccggc gtagctgacg gccagcgcca
    10261 gcagctgcga gcgctcgacg cggcgcttga acagcacgcg accgatcagc agcaccagcg
    10321 tcgggttcag gtacagcacg aggcgctcga ggcccgcgct gatgtaggcc aggcccgcga
    10381 agtcgaggaa gctcgcgagg tagtagcccg agaagcccag gcccgcgacg acccgccagt
    10441 cgcgcgcggc gagcccgggc ttgccgcgtc ccgcccacca cgcgatggcc gcgaacaacg
    10501 gcagcgcgaa cagcatgcgg tacatgatca gcgtcaccgc gtcgacgccg tagcggtacg
    10561 cgagcttgac gatgatcgcc ttgcccgaga aggcgatcga gccggccgtc gcgagcgcga
    10621 ggccgggcca caggcgttgc ggacgcggca ccggggtggt ggtggacagc gaggcaagcg
    10681 ttggcgtcgt ggtgctttca gaagccatgc gccagcttac ccggcgcggt gcggcagcgg
    10741 gatcgcgtct tggcaaggcc gcattcgcat ttgcatcgtg tgaacgggtg cgtcgttcga
    10801 cgtacgttcc tgtggcgtcg gcaccttcgc cattcttccg tccggagagg ctgctgccga
    10861 tgtgcggttg ccgcttcgcg caggcctgcg ctacgtgagc gacaggccgg gctgggactc
    10921 cgctctccgg cttgccgtgc gtcggaatcc ggcctggacg gcgcggattc agcgggttcc
    10981 gcggcttcga cttcgtcctc gcgctcgcgc gcgggccgcg ttcaatgtgg tgattggcgg
    11041 accggaggct cccacccgcg tcgcgccggt cgcgcctgcg atcgaagccc cagcccaccc
    11101 tgccgcgccg gcgtgtcagc cggtggatga aaacattcgc gggttttgtg aggggctttg
    11161 gcatcgacgg gccatcgagg ccgcgtcgat gcccgcggcc atcgctctgg cggggcgcga
    11221 ggcttgacgc gaacgacgga tcgcagtgcc gacgctcctt ctcgtgcccg acgagcgcgg
    11281 cgccttcggc gccacgctgc caggtggcaa gacgctggcg ggataccgcg ccaatgggac
    11341 aattcggcga tggttgcaag tgctggagac gacccggtgc tgacgtcgcg accgtcggac
    11401 gcgaagcccg aaagcgggcc ttcatcgccc catcgcgaac ggccgatcaa tccacgaaat
    11461 cgagttcctt cttgccgacg acgtgcttag aagcatcaag ccagaccacg caatagggtc
    11521 cgcctgcccc gaaggggaat tcgaccgagt ccagagaggt gtac
//
LOCUS       DATCRO010000082         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1047210, whole genome shotgun sequence.
ACCESSION   DATCRO010000082 DATCRO010000000
VERSION     DATCRO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047210"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1305)
                     /locus_tag="VIN75_02220"
     CDS             complement(163..1305)
                     /locus_tag="VIN75_02220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8973003.1"
                     /translation="MATVKRKLALVVPGGVHRSGTQDVIPAVLALVERLAARHELHVY
                     ALFQQREAARWPLRGAQVHAVGGTGRTAPWRALAAMAAEHRRAPFALVQALWAGPCGM
                     SAVAAGRLLSLPSAVHVAGGELVALHDIGYGGLLSRRGRWRERWVLRGAGAVTAASAP
                     MLAQIAGHGVQARRLPLGVDLAAWPARAPRPRAGGAPLRLLHLASLNRVKDQTTLLRA
                     VATAVAAGLPLRLDIVGIDTLDGAMQAQARALGLDEGLVRFHGFLPQRDCRAWVEQAD
                     VLVMASRHEAGPLVVLEAAVAGVPCAGTAVGHIAEWGGQAAALAVPVGDAAALAAAIL
                     RLGRDEPLRLALARRAQALALAQDADHTAAAFETLHAELLARRGRR"
     gene            1341..2228
                     /locus_tag="VIN75_02225"
     CDS             1341..2228
                     /locus_tag="VIN75_02225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8973004.1"
                     /translation="MTTLPDLQTQLRETQAAFDSVAADYDGPRGNNAAIQDMRAEMWR
                     WLDATFAPGSRLIDLGCGTGLDAVHLAGAGHRVLATDWSPRMVERTRERAAQAGLAGR
                     VQALAVGAHELHRLSGRELDLPGTGGPGDGVAAGFDGAYSNLGPLNCVPDLRAVSREC
                     ARLLRPGGALVFTVIGRHCPWEVLHYARQRRWARAAIRYASGTVPVGMNRQTIWTRYF
                     TPGEFHRDFAPEFDLVRVRGVCVFAPPPYLVDFKQRHPGWHDRLWRLDRRVAGWPGVR
                     RLGDHFLIVMRKRAEAGAA"
     gene            complement(2180..>2783)
                     /locus_tag="VIN75_02230"
     CDS             complement(2180..>2783)
                     /locus_tag="VIN75_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973005.1"
                     /translation="LTGLTWPLLAIAVALAEPLVLTLFGPAWREAAPAVPPLALLAAL
                     TALFTGAGPALTAVGRPAWAALPQALTLAAWLGLAATFTLPGGAAGTPRFAGWLLTAG
                     AVALPCQLWLHRRWLGLRPALLLRAVSPGAVIAVLTGATAWLGACAAPAGAPAPLRAA
                     AGLALALPAWWLARRMARRPTRLRRQAAPASARFLMTIRK"
BASE COUNT          338 a         1120 c         1041 g          284 t
ORIGIN      
        1 cgcctggagc cgccgctccc gcgccgcgcg catggcgggc tggctcctcg gcgcgccggc
       61 cagcgcggcg gcgcggttct cggtgcagca ggcactggcc tacggcgagc catgacgccg
      121 gcgaggacac cggcgtcgac gcggacccgg gcgtgccgcg tctcatcggc gcccccgccg
      181 ggcgagcagt tccgcatgca gcgtctcgaa ggcggcggcg gtgtggtcgg cgtcctgcgc
      241 gagggccagc gcctgcgcac gccgcgccag ggccaggcgc agcggctcgt cgcggcccag
      301 ccgcaggatc gccgcggcca gcgctgcggc atcgccgacc ggcaccgcca gcgcggccgc
      361 ctggccgccc cattcggcga tgtgcccgac ggcggtgccc gcgcacggca cgccggcgac
      421 ggccgcctcc agcaccacca gcgggccggc ctcgtggcgg gatgccatca ccagcacgtc
      481 ggcctgctcg acccaggcgc ggcagtcgcg ctgcggcagg aagccgtgaa agcgcaccag
      541 gccctcgtcc aggcccagcg cgcgcgcctg cgcctgcatg gcgccgtcca gggtgtcgat
      601 gccgacgatg tccaggcgca gcggcagccc ggccgcgacc gcggtcgcga cggcgcgcag
      661 cagcgtggtc tggtccttga cccggttcag gctcgccagg tgcagcagcc gcagcggcgc
      721 gccgccggcg cgcggccgcg gcgcgcgtgc cggccaggcc gcgaggtcga cgcccagcgg
      781 cagccgccgc gcctgcacgc cgtgcccggc gatctgcgcc agcatcggcg cgctggccgc
      841 ggtcaccgcg ccggcgccgc gcagcaccca gcgctcgcgc cagcggccgc ggcggctcag
      901 caggccgccg tagccgatgt cgtgcagcgc gaccagctcg ccgccggcca cgtgcaccgc
      961 gctgggcagg gacaggagcc gtcccgccgc caccgcgctc atcccgcagg gacccgccca
     1021 cagcgcctgc accagcgcga acggcgcgcg ccgatgctcg gccgccatgg ccgccagcgc
     1081 gcgccagggc gccgtgcggc ccgtgccgcc gaccgcgtgc acctgcgccc cgcgcagcgg
     1141 ccatcgcgcc gcctcgcgct gctggaacag cgcgtagaca tgcagctcgt gccgcgccgc
     1201 cagccgctcc accaaggcca gcaccgccgg gatcacgtcc tgcgtgcccg agcgatgcac
     1261 gccgccgggc acgaccagcg cgagcttgcg cttcaccgtc gccattccga ttccttcacc
     1321 ttgtccatcc gcttcctgcc atgaccacgc ttcccgacct tcagacccag ctgcgcgaga
     1381 cgcaggccgc cttcgacagc gtcgccgccg actacgacgg cccgcgcggc aacaacgccg
     1441 cgatccagga catgcgcgcc gagatgtggc gctggctcga cgcgaccttc gcgcccggca
     1501 gccggctgat cgacctgggc tgcggcaccg gcctggacgc ggtccacctg gccggggccg
     1561 gccaccgggt gctggccacc gactggtcgc cgcgcatggt cgagcgcacg cgcgagcgcg
     1621 cggcgcaggc cggcctggcc ggccgcgtcc aggcgctggc cgtcggcgcg catgagctgc
     1681 atcggctctc cggccgggaa ctcgacctgc cggggaccgg cggacccggc gacggtgtcg
     1741 cggccggctt cgacggcgcg tactccaacc tcggtccgct gaactgcgtg cccgacctgc
     1801 gcgccgtctc gcgcgaatgc gcccggctgc tgcggccggg cggcgcgctg gtcttcaccg
     1861 tcatcggccg ccattgcccg tgggaggtgc tgcactacgc gcgccagcgc cgctgggcgc
     1921 gcgcggcgat ccgctacgcc agcggcaccg tgccggtggg catgaaccgc cagacgatct
     1981 ggacgcgtta cttcacgccg ggcgagttcc atcgtgactt cgcgcccgag ttcgacctcg
     2041 tgcgggtgcg cggcgtctgc gtgttcgcgc cgccgcccta cctggtcgac ttcaagcagc
     2101 gccatccggg ctggcacgac cggctgtggc ggctggaccg ccgggtggcc ggctggccgg
     2161 gcgtgcgtcg gctcggcgat catttcctga tcgtcatgag aaagcgggcg gaggccggcg
     2221 cggcctgacg gcgaaggcgc gtcggccgcc tggccatccg ccgcgccagc caccaggccg
     2281 gcaacgcgag cgcgagcccg gccgccgcgc gcagcggtgc cggcgctccg gccggcgcgg
     2341 cgcaggcgcc cagccacgcg gtggcgccgg tcaggacggc gatgacggcg ccgggcgaca
     2401 ccgcgcgcag cagcagcgcc gggcgcaatc cgagccagcg ccggtgcagc cacagctggc
     2461 agggcagcgc cacggcgccc gccgtcagca gccagcccgc gaagcgaggc gtgccggccg
     2521 cgccgccggg caatgtgaac gtggcggcca gcccgagcca ggcggcgagc gtcaaggcct
     2581 gcggcagcgc cgcccaagcg ggccggccga cggccgtcaa cgcggggccc gcgccggtga
     2641 acagggcggt cagcgcggcc agcagcgcca gcggcggcac ggcgggcgcg gcctcacgcc
     2701 aggccgggcc gaacagcgtc agcaccagcg gctcggccag cgccaccgcg atggcgagca
     2761 ggggccaggt caggccagtg agc
//
LOCUS       DATCRO010000083         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_698576, whole genome shotgun sequence.
ACCESSION   DATCRO010000083 DATCRO010000000
VERSION     DATCRO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698576"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VIN75_02235"
     CDS             complement(<1..1312)
                     /locus_tag="VIN75_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8973006.1"
                     /translation="MIHHSSPLRLLRAAMAALLLAASAGAVAGASRSPCTPKPVSDEP
                     KSQAPDALSVHVDAARPLALANWDVNWWQRRRVWVTLAASNEGDAPTQVLPQMVVDAR
                     PDGGAMMALFGLPLALPAHGRATQRLAIYVPDDARTLGVRTLVATPAQPVAVSFTLEC
                     SDSRYDPGEFAPAVAPLLDEAMKTWFNGLVDPLSDPNATVDLARRLSSGAQEPADVAW
                     TMRALMQAVHDDHGFYALPGEALPARPALVTRAPELELRGDGTAVLRLHALDTTSAAA
                     ALAWATSLHEGIAAVAARHPRGWIIDLRDHDGQTPWPSLAALSTLLDGPAVGHFASRR
                     GTQEWIVERGAARIAGGPALVDTQPPPEPDFRGPVALLIGRGTRDAGEDVAVAFRGRP
                     NTRFFGAPTAGFPQQGVQPHRLADGSTLGVLEVRAADRTGVVQRE"
     gene            complement(1373..2185)
                     /locus_tag="VIN75_02240"
     CDS             complement(1373..2185)
                     /locus_tag="VIN75_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEY8973007.1"
                     /translation="MSMHAADVHALSDAVRAIERPGVGARLANLVGSPIERLVGMLPK
                     RASGVISAAARKAIHGALNLSLRTLSTNDPLAPGAPPPASNGWHKVASAASGAVGGAF
                     GLAALAIELPVSTTIMMRSIADVARSEGADLADRQTQLECVMVLALGGNATSDDAAEV
                     GYFAVREAMTRAVSSAAAHLARHGLAKEGAPALVRLITLIAEHYSVNVTEKAAAQMVP
                     LIGAVGGAAVNTIFIDHFQTVARGHFAVRRLERKYGEATVREKYRELAAALA"
     gene            2254..2670
                     /locus_tag="VIN75_02245"
     CDS             2254..2670
                     /locus_tag="VIN75_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973008.1"
                     /translation="MARPTAPSLALACALAAAGCAAPAGSMDARYEASLLRWQGATRA
                     QLVASWGAPTLAQATPDGEVLTWIVRTDIDGRTASGTPPVVAVTRVGGTGATIAGATP
                     ALTPPAAPITCTTHFVLTKGRVASWKFEGMGCGAPG"
     gene            complement(2846..4702)
                     /locus_tag="VIN75_02250"
     CDS             complement(2846..4702)
                     /locus_tag="VIN75_02250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8973009.1"
                     /translation="MPKPSAAETAKAALRRLALSKLEPTPENYARAWAEEGGAPAVAP
                     PAPAASAAAASTSVAKAKGVYDRIAARLFEDGTQREEFAQALQRADWDAARGHVERSA
                     ESQAALSQGLALTIEKLARGLERGGRQWTAARKKDSLQRVLEANRSDIARLHQRMKQL
                     VASWDGDVADETVDVDAGGGHEAPPSRPMELLDLGSGTISEGGSIELQGGIGHAPSER
                     PGAHGDWPPVLAQYDATLHVALPADDARATALSHELKDLQADLARDGATARLRAQAEA
                     LCERARRLLAHRHHLVDELHRLTLEMSAGLAEVAEEGSWVKGQTESLKQSLGEQPSVR
                     SVRAASEMLASTRARQQRVRAERDSARVALKDAISQMLTELDALGDHTGHFSDSVARY
                     VDTISHADSLESLASVVRQMMEEGRAVHGLVSETQERLNEGRQRAAELEEQVRSLESE
                     LRRLSDEVSTDVLTEVANRRGLMQAFEVETARLERQGGELAIGLLDIDNFKKLNDTLG
                     HSVGDIALKSLAGHVQKQLRAVDVVARFGGEEFVVMLPGVAVDEAQVTLTRLQRTLSA
                     SLFMHDGREVFVTFSAGVTRYRPGEALEAALERADEALYEAKRTGKNRTCIG"
     gene            complement(4742..>5606)
                     /locus_tag="VIN75_02255"
     CDS             complement(4742..>5606)
                     /locus_tag="VIN75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973010.1"
                     /translation="GRGREKLALESALHKAIERDELVLYYQPKVDVANGKMVGVEALM
                     RWRRGDLLIQPADFIPVAEETGLIVPMSEWALRQAAKQARIWADAFGFADSIAVNMPN
                     RMFERTDLIEQISSAVREEGIPHSMIQLEITETGLMKDLQRVIPSLHRLTEIGVQIAV
                     DDFGTGYSSLAYLTSLPIAEVKIDRSFVRDLGVLPQSLAVVTAIIALAKSLDLKVVAE
                     GVENQRQQDVLYGEGCSLMQGFLFSKPMPPEDVQTWMESVALPRKAVAAPSAAKVRAN
                     AGEVVDVGRRS"
BASE COUNT          713 a         2067 c         2034 g          792 t
ORIGIN      
        1 gctcgcgctg gacgaccccg gtgcggtcgg cggcgcgcac ctcgagcacg cccagcgtgc
       61 tgccgtcggc caggcggtgc ggctggacgc cctgttgcgg gaagccggcg gtcggcgcgc
      121 cgaagaagcg tgtgttcggg cggccgcgga aggcgacggc gacgtcctcg ccggcgtcgc
      181 gcgtgccgcg cccgatcagc agcgcgaccg gcccgcggaa gtcgggctcg ggcggcggct
      241 gggtgtcgac cagcgccggg ccgccggcga tgcgcgcggc gccgcgctcg acgatccact
      301 cctgcgtgcc gcgccggctg gcgaagtggc ccaccgcggg gccgtccagc aaggtgctga
      361 gcgcggccag cgacggccac ggggtctgcc cgtcgtggtc gcgcaggtcg atgatccagc
      421 cgcgcggatg acgcgccgcc acggcggcga tgccttcatg cagcgacgtg gcccaggcga
      481 gcgcggcggc tgcggacgtc gtgtcgagcg cgtgcaggcg cagcacggcc gtgccgtctc
      541 cgcgcagctc gagctcgggc gcgcgcgtga cgagcgcggg ccgcgccggc agcgcctcgc
      601 cgggcaacgc gtagaagcca tggtcgtcgt gcaccgcctg catcagggcg cgcatcgtcc
      661 aggcgacgtc ggcgggctcc tgcgcgccgc tggacaggcg gcgtgcgagg tcgacggtgg
      721 cgttcgggtc ggacagcggg tcgaccaggc cgttgaacca ggtcttcatc gcctcgtcca
      781 gcaacggcgc gacggccggc gcgaactcgc ccgggtcgta gcgcgagtcc gagcattcca
      841 gcgtgaacga cacggccacg ggctgggcgg gcgtggccac cagcgtgcgc acgcccagtg
      901 tcctggcgtc atcgggcacg tagatcgcaa gacgctgcgt ggcgcggccg tgcgcgggca
      961 acgccagggg caggccgaac aacgccatca tcgcgccgcc atcgggacgc gcgtcgacca
     1021 ccatctgcgg cagcacctgc gtcggcgcgt cgccctcgtt gctggcagcc agcgtgaccc
     1081 acacgcggcg gcgctgccac cagttcacgt cccagttggc gagcgcgagc ggcctggcgg
     1141 cgtcgacatg caccgacaac gcatcgggcg cctgcgactt gggctcgtcg ctgaccggct
     1201 tgggcgtgca cggcgaacgc gacgcgcccg cgacggcgcc ggcggacgcg gccagcagca
     1261 gcgccgccat ggcggcacgc aagagtcgga gcggcgacga gtggtggatc atgcggacgg
     1321 gcggcgcgga accagcccgg aatatacggc cccgcgctga agacgcggct tctcaggcca
     1381 gcgccgccgc gagctcgcgg tacttctcgc gcaccgtggc ctcgccgtac ttgcgctcca
     1441 ggcggcgcac ggcgaagtgg ccgcgcgcga ccgtctggaa gtgatcgatg aagatcgtgt
     1501 tgacggccgc gccgcccacc gcgccgatca gcggcaccat ctgcgcagcg gccttctcgg
     1561 tcacgttcac ggagtagtgc tcggcgatca gcgtgatcag ccgcaccagc gcgggcgcgc
     1621 cctccttggc caggccgtgc cgcgccagat gcgccgcggc gctggagacg gcgcgcgtca
     1681 tcgcctcgcg caccgcgaag tagccgacct cggcggcatc gtcgctcgtc gcgttgccgc
     1741 ccagggcgag caccatcacg cactcgagct gggtctggcg gtcggccagg tcggcaccct
     1801 cgctgcgcgc gacgtcggcg atcgagcgca tcatgatcgt cgtcgacacc ggcagctcga
     1861 tggccagcgc cgcgaggccg aacgcgccgc ccaccgcgcc actggccgcg ctcgccacct
     1921 tgtgccagcc gttggacgcc ggcggcggcg cgcccggcgc gagcgggtcg ttggtggaca
     1981 gcgtgcgcag cgacaggttc agcgcgccgt ggatggcctt gcgcgccgcc gccgagatga
     2041 cacccgatgc gcgcttgggc agcatcccca ccagccgctc gatcggcgag ccgacgaggt
     2101 tggccagccg cgcgccgacg ccggggcgct cgatcgcgcg gacggcgtcg gacaaggcgt
     2161 ggacatcggc ggcgtgcatg ctcatcggcg gatcctcgac gggaagatac cctcctttcg
     2221 gcaatccgcg cgccgcgggt gtccaatgca cgcatggccc gaccgaccgc cccgtccctc
     2281 gcgctcgcgt gcgcactggc cgcggcgggc tgcgccgcgc cggccggatc gatggatgcg
     2341 cgctacgagg cctcgctgct gcgctggcag ggcgcgacgc gcgcgcagct cgtggcgtcg
     2401 tggggcgcgc cgacgctggc acaggccacg cccgacggcg aggtgctgac gtggatcgtg
     2461 cgcacggaca tcgacggccg gacggcctcc ggcacgccgc ccgtcgtcgc cgtcacccgc
     2521 gtcggcggca ccggcgcgac catcgccggc gccacgcccg cactcacgcc gccggcggcg
     2581 cccatcacgt gcaccacgca tttcgtgctg acgaaagggc gcgtggcgtc gtggaagttc
     2641 gagggaatgg ggtgcggggc gcccgggtga ccccggccga cgcgccccgg actcgtaggg
     2701 cgggttagcc gcaggtgtaa cccgccgctc gccggcctgc atatccgccg tcgatcgggc
     2761 tggcgcatcg aggcgagccg aatggcgggt tacgcctgcg gccaacccgc cctgctggcg
     2821 gttcggaagt gcgtggttca ggtcgctacc cgatgcacgt ccggttcttg cccgtccgct
     2881 tggcctcgta cagcgcctcg tctgcgcgtt ccagcgccgc ctccagcgcc tcgcccgggc
     2941 ggtagcgcgt gacgccggcg gagaaggtga cgaagacctc gcggccgtcg tgcatgaaca
     3001 ggctcgccga cagcgtgcgc tgcaggcgcg tcagcgtgac ctgcgcctcg tccaccgcga
     3061 cgcccggcag catcacgacg aactcctcgc cgccgaagcg cgcgacgacg tcgaccgcgc
     3121 gcaactgctt ctgcacgtgg ccggccagcg acttcagcgc gatgtcaccc accgagtggc
     3181 ccagcgtgtc gttgagcttc ttgaagttgt cgatgtccag caggccgatc gccagttcgc
     3241 cgccctggcg ctccaggcgc gccgtctcga cctcgaaggc ctgcatcagc ccgcgccggt
     3301 tggcgacctc ggtgagcacg tcggtggaca cctcgtcgga cagccgccgc agctcggact
     3361 ccagcgagcg cacctgctcc tcgagttcgg ccgcgcgctg gcggccctcg ttcaatcgct
     3421 cctgcgtctc gctgaccagg ccatgcacgg cgcggccctc ttccatcatc tggcgcacga
     3481 cgctggccag gctctccagc gagtccgcgt gcgagatcgt gtcgacgtag cgcgccacgc
     3541 tgtccgagaa gtggccggtg tggtcgccca gcgcgtcgag ctcggtgagc atctgcgaga
     3601 tcgcgtcctt gagtgccacg cgcgcgctgt cgcgttcggc gcgcacgcgt tgctggcgcg
     3661 cgcgcgtgct cgctagcatc tcgctggcgg cgcgcaccga gcgcacgctc ggctgctcgc
     3721 ccaggctctg cttcaggctc tcggtctggc ccttgaccca gctgccctcc tcggccacct
     3781 cggccaggcc ggcgctcatc tccagcgtca gccggtgcag ctcgtcgacc aggtgatggc
     3841 gatgcgccag caggcggcgc gcgcgctcgc acagcgcctc ggcctgcgcg cgcaggcgcg
     3901 cggtggcgcc gtcgcgcgcg aggtcggcct gcaggtcctt gagctcgtgc gacagcgccg
     3961 tggcgcgcgc gtcgtcggcg ggaagggcca cgtgcagcgt cgcgtcgtac tgcgcgagca
     4021 ccggcggcca gtcgccgtgc gcgcccgggc gttcgctggg cgcgtggccg atgccgccct
     4081 gcagctcgat cgagccgccc tcggagatcg tgccgctgcc caggtcgagc agttccatgg
     4141 gacgcgacgg tggcgcttcg tgcccgccgc ccgcatcgac gtcgaccgtc tcgtcggcga
     4201 cgtcgccgtc ccatgacgcc accagctgct tcatgcgctg gtgcagccgc gcgatgtcgc
     4261 tgcggttggc ctccagcacg cgctgcaggc tgtccttctt gcgcgccgcc gtccactggc
     4321 gcccgccgcg ctccaggccg cgcgcgagct tctcgatggt cagggccagg ccctgcgaca
     4381 acgcggcctg cgattcggcc gagcgctcga cgtggccgcg cgccgcgtcc cagtcggcgc
     4441 gctgcagcgc ctgcgcgaac tcctcgcgct gcgtgccgtc ctcgaacagc cgcgcggcga
     4501 tgcggtcgta cacgcccttg gccttggcga cggacgtgga cgcggccgcc gcggaggcgg
     4561 ccggcgcggg cggcgccacg gccggcgcgc cgccctcctc ggcccaggcg cgggcatagt
     4621 tctcgggcgt gggctccagc ttggacaacg cgaggcggcg caacgcggcc ttggcggtct
     4681 cggcggcgga gggtttgggc atggcgatgt cgacggctgg cggcgcgagg gccgcatgag
     4741 attacgagcg ccgcccgacg tcgaccactt cgccggcgtt ggcgcgcacc ttcgctgcgg
     4801 agggcgccgc caccgccttg cgcgggagcg cgacgctctc catccaggtc tggacgtcct
     4861 ccggcggcat cggcttgctg aacaggaacc cctgcatcag cgagcagcct tccccgtaga
     4921 ggacatcctg ctgcctctgg ttttcgacgc cttcggccac caccttgagg tccagcgact
     4981 tcgccagcgc gatgatcgcg gtaacaaccg caagactctg tggcagcacg cccaggtcgc
     5041 gcacgaagct gcggtcgatc ttgacttccg cgatcggcag cgacgtcagg tacgcgagcg
     5101 acgaatagcc ggtgccgaag tcgtcgacgg cgatctgcac gccgatctcg gtgagccggt
     5161 gcagcgacgg gatcacgcgc tgcaggtcct tcatcaggcc cgtctcggtg atctccagct
     5221 ggatcatgct gtgcgggatg ccttcctcgc gcacggccga cgagatctgc tcgatcaggt
     5281 cggtgcgctc gaacatccgg ttgggcatgt tgacggcgat ggagtcggca aagccgaacg
     5341 cgtcggccca gatgcgcgcc tgcttggcgg cctggcgcag ggcccactcg ctcatgggca
     5401 cgatcaggcc ggtttcctcg gcgacgggga tgaagtcggc cggctggatc agcaggtcgc
     5461 cgcgacgcca gcgcatcagc gcctcgacgc ccaccatctt gccgttggcc acgtccacct
     5521 tgggctggta gtacagcacc agctcgtcgc gctcgatggc cttgtgcagc gcgctctcga
     5581 gcgccagctt ctcgcgcccg cggccg
//
LOCUS       DATCRO010000084         6607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_721055, whole genome shotgun sequence.
ACCESSION   DATCRO010000084 DATCRO010000000
VERSION     DATCRO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721055"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIN75_02260"
     CDS             <1..462
                     /locus_tag="VIN75_02260"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY8973011.1"
                     /translation="SLAIVAPAGVTTSYALSGAPEGLAVDAAGALRWAAPVAGSYGFT
                     ATATTAIGLSASARYTLAVVKVNHAPTLASGSVAVPPGVALHLALAASDIDGDALSYS
                     MTGAPRGLSLSSGGVLGWTTTVRGRWVLRVVARDAQGLASAPAAIVLNVSN"
     gene            complement(470..2143)
                     /locus_tag="VIN75_02265"
     CDS             complement(470..2143)
                     /locus_tag="VIN75_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973012.1"
                     /translation="MLRITELRLPLDHAEDALRPAIVARLGVRDAELASFTVFKRSYD
                     ARKKTAIVLIYTIDCELAVDESAVLARFAGDPHVRVAPDTNYRFVGRAAEGFFDGASA
                     PRQRPVVVGFGPCGIFAALILAQMGLEPIVLERGKPVRERTKDTWGLWRQGKLDPESN
                     VQFGEGGAGTFSDGKLWSQISDPRHLTRKVLTEFVKAGAPEEILFVAKPHIGTFRLVS
                     MVEKIRADIVALGGEIRFEQRVTDVLLEGRGDEKRIRGLRLASGEELRTDHVVLALGH
                     SARDTFAMLHERGVRMEAKPFSVGYRIEHPQSLIDRARFGPNAGNQILGAADYKLVHH
                     ASNGRAVYSFCMCPGGTVVAATSEERRVVTNGMSQYSRNERNANAGIVVGIDPQDYRQ
                     DGKASGPVNPLDGIAFQRFWESRAYELGGGGYVAPGSLVGDFIKRQRSSAFGEVLPSY
                     KPGVHLTDLQEKGRGSLPEYALDAIREALPAFERQIKGFSLPDAVLTGVETRTSSPLR
                     IPRGKDYQSLNVRGLYPAGEGAGYAGGIMSAGVDGIEVAEALGAALLAS"
     gene            complement(2342..2506)
                     /locus_tag="VIN75_02270"
     CDS             complement(2342..2506)
                     /locus_tag="VIN75_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973013.1"
                     /translation="MGNKIVTEADRKATPRPALPIGVTATTGKGQKRSLERGAHTRSK
                     KNPGKRARQG"
     gene            2785..3270
                     /locus_tag="VIN75_02275"
     CDS             2785..3270
                     /locus_tag="VIN75_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973014.1"
                     /translation="MKKLHLVLAALVAVAAGAAQAQVTVGASVNINVPGVYGRVDIGA
                     PAPGVAFVAPPVVYAEPVVVAPTPVTVQQRPIYLYVPEWQQRDWRHHCGAYGACGQRV
                     YFVQESWVRDRYVQHHPHDHRFDHWEGEHDHGRRGHDHDHDDHDDDHDHGHGHGHGHD
                     H"
     gene            3438..>6607
                     /locus_tag="VIN75_02280"
     CDS             3438..>6607
                     /locus_tag="VIN75_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8973015.1"
                     /translation="MPDRQQAAGRGKATVRRAGALESTLPPRYKRQAWLAVVALLVFM
                     ALYAALAGWFLRTAWRLSLGSSDPQFFGLVAAACAVLIAAFMIKGLFFIRRGKPGGLV
                     ELRPDDQPRLFEFLHQLADDAGAPRPHKVYVSARVNAAVFYDLSLVNLLLPSRKNLEI
                     GLSLVNALTLGELRAVLAHEFGHFGQRAMAVGRWVYVAHQVTAQLVARRDKLDAFVDG
                     LSRMDLRIAWVGWLLQTIVWAIRSLIDLAFRGVLLLESALSREMELQADLVAVSLTGS
                     DALVHALSKLRAADDSFDRAVGFAQGEQARGAPVADLFEVHAHLMKRMAAILDDPLYG
                     RVPLVPLADPAAHRVFRQELTQPPRMWQSHPQNHEREANAKKTYVPAPLDERSAWLLF
                     DAVPALRERVTRELLPPGDKAVAPIAETIARVDARFERPSLQRRYQGVYLGRAVTRSV
                     DDIEDMLRGAAGVADLSGLYPKSLAATVERVRELDRELAQLRALDAGVVEASGGAIRY
                     RGDDVARRELPRLIARVSAERDEARAQLDAHEARARQAHLAAARVVDAAAAPGAPTHE
                     AALRGLLAVLHYAEHGEAVARDLQAAMWVRFRMVTAGGKVTHDTLVPLINDCNHLQQA
                     VDALHLERDKLLLSPAVLARLDATSWSEAFGSFDLPLASSQNLQKWMEVVDGWIDSSA
                     GSLGALRNAALDELLLAEQALAAQVRDGTPAPAAEAACVVPVRYRRLLVQHEQVRKVT
                     LTWWQRFLRASGRGPAFLRLAIAGGIVGAVLTVGADIGSATLVIYDGLDIPVRVQVGE
                     TGHDVEPGSPLKLDVEPDSKLAIVTTTRDGREIERFDAKIQGGFGTQVYNVAGASPLV
                     RWTAVYGPAREEPPQPLGAPRLVAVSVDHLFEDPPRSVETRHGEGAVRHVLQGFAGST
                     PQELLGALGSDVATAHHVALVHARWDVDHQGDLESWFAAASGEPGLAAVLQDRLARSP
                     DSVALARAAQYLAGEGPAHEAACARDRARAAQRPDDGDRAYLAIRCEPDERSGADFIA
                     AHQRWPTNPWLASAAGWAYANDERWD"
BASE COUNT          870 a         2387 c         2423 g          927 t
ORIGIN      
        1 tcgttggcca tcgtggcgcc cgcgggcgtg accacgagct acgcgttgtc cggcgcgccc
       61 gagggtctcg cggtcgatgc cgccggcgcg ctgcgctggg cggcgcccgt cgccggcagc
      121 tacgggttca cggcgacggc gacgaccgcc atcggcctgt cggccagcgc acgctacacg
      181 ctggccgtgg tgaaggtgaa ccatgcgccg acgctggcct cgggcagcgt ggccgtgccg
      241 cccggcgtgg cgctgcacct cgcgctggcg gcctcggaca tcgacggcga tgcgctgagc
      301 tactcgatga ccggcgcgcc gcgcgggctg tcgctgtcga gcggcggcgt gctgggctgg
      361 acgacgacgg tgcgcgggcg ctgggtgctg cgcgtggtgg cgcgcgatgc gcagggcctg
      421 gcgagcgcgc cggccgcgat cgtgctgaac gtgtcgaact gaaggacgct caactggcca
      481 gcagcgcggc gccgagcgcc tcggcgacct cgatgccgtc gacgcccgcc gacatgatgc
      541 cgccggcata gcccgcgcct tcgccggccg ggtacaggcc gcgcacgttc aggctctggt
      601 agtccttgcc gcgcgggatg cgcagcggcg acgacgtgcg cgtctccacg ccggtgagca
      661 ccgcgtcggg cagcgagaag cccttgatct ggcgctcgaa cgccggcagc gcctcgcgga
      721 tcgcgtcgag cgcgtactcg ggcaggctgc cgcggccctt ctcctgcagg tcggtcaggt
      781 gcacgccggg cttgtacgac ggcagcacct cgccgaaggc gctggaacgc tggcgcttga
      841 tgaagtcgcc gacaagcgaa cccggcgcca cgtagccgcc gccgcccagc tcgtaggcgc
      901 gcgattccca gaagcgctgg aacgcgatgc cgtccagcgg gttcaccggg ccggacgcct
      961 tgccgtcctg gcggtagtcc tgcggatcga tgcccacgac gatgccggcg ttggcgttgc
     1021 gctcgttgcg cgagtactgg ctcatgccgt tcgtgacgac gcggcgttcc tccgacgtcg
     1081 cggccacgac ggtgccgccg gggcacatgc agaagctgta gacggcgcgg ccgttgctgg
     1141 cgtggtgcac gagcttgtag tcggcggcgc ccaggatctg gttgccggcg ttcgggccga
     1201 agcgcgcgcg gtcgatcagg ctctgcgggt gctcgatgcg gtagccgacc gagaacggct
     1261 tggcctccat gcgcacgccg cgctcgtgca gcatcgcgaa ggtgtcgcgc gcgctgtggc
     1321 ccagcgccag cacgacgtgg tcggtgcgca gttcctcgcc ggaggccagg cgcagtccgc
     1381 ggatgcgctt ctcgtcgccc cggccttcga gcaggacgtc ggtcacgcgc tgctcgaagc
     1441 ggatctcgcc gccgagcgcg acgatgtccg cacggatctt ctcgaccatg ctcacgagcc
     1501 ggaacgtgcc gatgtgcggc ttggcgacga agaggatctc ctcgggcgcg ccggccttca
     1561 cgaactcggt gagcaccttg cgcgtgagat ggcgcgggtc gctgatctgg ctccacagct
     1621 tgccgtccga gaaggtgccg gcgccgccct cgccgaactg cacgttcgac tccggatcca
     1681 gcttgccctg gcgccacagg ccccaggtgt ccttggtgcg ctcgcgcacc ggcttgccgc
     1741 gttccagcac gatgggctcg agacccatct gcgcgaggat gagggccgcg aagatgccgc
     1801 acggcccgaa gccgacgacc acggggcgct gccgcggcgc gctcgcgccg tcgaagaagc
     1861 cctcggccgc gcggccgacg aagcggtagt tggtgtcggg cgcgacgcgc acgtgcgggt
     1921 cgcccgcaaa gcgcgcgagc acggccgatt catcgacggc gagctcgcag tcgatggtgt
     1981 agatcagcac gatggccgtc ttcttgcgcg cgtcgtagct gcgcttgaag acggtgaacg
     2041 acgccaactc cgcgtcccgc acgcccaggc gcgcgacgat cgcgggacgc agggcgtcct
     2101 cggcgtggtc cagcggcagg cgcagttcgg tgattcgaag catggtgcga tgtccggcgc
     2161 acgcgcggag cgcggcacaa cgaacaaagg agcccgaagg ctcctggttc acgccgtcag
     2221 ggcgtcagtg gtcgagggca ccgcggcgga tgagccgcga gtgcgtgtct ccagccgacc
     2281 ggggccggca gaaggtcggc cctggcggcc tgaaccgggt ccagcctccg gctggccccg
     2341 ttcaaccttg gcgagcgcgc ttgcccgggt tcttcttgct gcgggtgtgg gcgccgcgct
     2401 cgaggctgcg cttctggccc ttgccggtgg tggcggtgac gccgatcggc agcgccggac
     2461 gcggggtggc cttgcggtcg gcttcggtaa cgatcttgtt gcccatgatt ggatccggtc
     2521 ttgtaaagct gtaaggggtc gaacccggca ttctcgcaca aatgcggcgg tttggcacct
     2581 cccgggctgg gcgggcgcgc cagatcgtgc cggacggggc attcgcctgc gtccaagtcg
     2641 gacgccactg acgagtagcg ggccccgatg tctgtaaagt tcgattacgt tgacggcgtc
     2701 aacgacccgg tcgcgcaggc cgttttagat gcaagctcgg gttcagcatc ccgccccccg
     2761 tgcagcccag aaggaacaag caccatgaag aagctccacc tcgttctcgc cgccctcgtg
     2821 gcggtcgccg ccggcgccgc ccaggcccag gtcaccgtgg gcgccagcgt gaacatcaac
     2881 gtccccggcg tctatggccg cgtcgacatc ggcgctccgg ccccgggcgt ggccttcgtg
     2941 gcgcccccgg tggtctacgc cgagcccgtc gtggtcgccc cgacgccggt caccgtgcag
     3001 cagcgcccga tctacctgta cgtgcccgaa tggcagcagc gcgactggcg ccaccactgc
     3061 ggcgcgtacg gcgcctgcgg ccagcgcgtg tacttcgtgc aggagagctg ggtgcgcgac
     3121 cgctacgtcc agcaccaccc gcacgaccat cgcttcgacc actgggaagg cgagcatgac
     3181 cacggccgcc gcggccacga ccatgaccac gacgaccatg acgacgacca cgaccacggc
     3241 catggccatg gtcacggtca cgaccactga ccgcccaccg gcggcccgct gacggccgcc
     3301 ctcccacgca gccaccgccc gcgccccccg cgggcggtgc gctttcgagg gaaaacgtcc
     3361 tcccgcccgg cgccgtcgca agtcgacaat aggccctgga aggagcaccc gccgtacgag
     3421 cggggcaggg gaggacgatg ccggatcgac agcaggccgc gggacgcggc aaggccaccg
     3481 tgcgccgtgc gggcgcgctc gaatcgacgc tgccgccgcg ctacaagcgc caggcctggc
     3541 tggccgtcgt cgcgctgctc gtgttcatgg cgctgtacgc cgcgctggcc ggctggttcc
     3601 tgcgcaccgc ctggcgcctg agcctgggca gcagcgatcc gcagttcttc ggcctggtgg
     3661 ccgcggcgtg cgccgtgctc atcgcggcgt tcatgatcaa gggcctgttc ttcatccggc
     3721 gcggcaagcc cggcggcctg gtcgaactgc gacccgacga ccagccgcgg ctgttcgagt
     3781 tcctgcacca gctcgccgat gacgccggcg cgccgcgccc gcacaaggtc tacgtgtccg
     3841 cgcgcgtcaa cgccgcggtg ttctacgacc tgtcgctggt caacctgctg ctgccctcgc
     3901 gcaagaacct cgagatcggc ctgagcctgg tcaacgcgct cacgctcggc gagctgcgcg
     3961 ccgtgctggc gcacgagttc ggccacttcg ggcagcgtgc gatggcggtg ggccgctggg
     4021 tgtacgtcgc gcaccaggtc accgcgcagc tggtcgcgcg gcgcgacaag ctcgacgctt
     4081 tcgtcgacgg cctctcgcgc atggacctgc gcatcgcgtg ggtcggctgg ctgctgcaga
     4141 cgatcgtgtg ggccatccgc tcgctgatcg acctcgcgtt ccgcggcgtg ctgctcctgg
     4201 agtcggccct gtcgcgcgag atggagctgc aggccgacct cgtggccgtc agcctcaccg
     4261 gcagcgacgc gctggtgcac gcgctgtcca agctgcgtgc ggcggacgac agcttcgacc
     4321 gcgccgtcgg cttcgcgcag ggcgagcagg cgcgcggcgc gcccgtggcc gacctgttcg
     4381 aggtgcacgc gcatctcatg aagcgcatgg ccgcaatcct cgacgacccg ctgtacggcc
     4441 gcgtgccgct ggtcccgctc gccgatccgg ccgcgcaccg cgtcttcagg caggagctga
     4501 cccagccgcc gcgcatgtgg cagtcgcacc cgcagaacca cgagcgcgag gccaacgcga
     4561 agaagaccta cgtgcccgcg ccgctggacg agcgctccgc ctggctgctg ttcgacgcgg
     4621 tgcccgcgct gcgcgagcgc gtgacgcgcg agctcctgcc gcccggcgac aaggccgtcg
     4681 cgccgatcgc cgagacgatc gcgcgcgtcg acgcccgctt cgagcgcccg agcctgcagc
     4741 ggcgctacca gggcgtctat ctcggccgcg ccgtcacgcg ctcggtggac gacatcgagg
     4801 acatgctgcg cggcgccgcc ggcgtcgcgg acctttcggg gctgtatccg aagtcgctgg
     4861 cggcgacggt cgaacgcgtg cgcgagctcg atcgcgagct ggcgcagctg cgcgcgctcg
     4921 acgccggcgt ggtcgaggcc agcgggggcg ccatccggta tcgcggcgac gacgtggccc
     4981 gccgcgagct gccccggctg atcgcccggg tgtcggccga gcgcgacgag gcgcgcgcgc
     5041 agctggacgc gcacgaggcc cgcgcgcgcc aggcgcacct ggccgcggcg cgcgtggtcg
     5101 acgccgcggc cgcgccgggc gcgcccacgc acgaggccgc gctgcgcggg ttgctggccg
     5161 tgctgcacta cgccgagcac ggcgaggccg tggcgcgcga cctgcaggcg gcgatgtggg
     5221 tgcgcttccg gatggtcacc gccggcggca aggtcacgca cgacacgctg gtgccgttga
     5281 tcaacgactg caaccacctg cagcaggccg tcgacgcact gcacctggag cgcgacaagc
     5341 tgctgctgtc gccggcggtg ctggcgcggc tggacgcgac gagctggagc gaggccttcg
     5401 gcagcttcga cctgccgctg gcctcctcgc agaacctcca gaagtggatg gaggtggtcg
     5461 acggctggat cgattcgtcg gccggctcgc tcggcgcgct gcgcaacgcg gcgctcgacg
     5521 agctcctgct ggccgagcag gccctggccg cgcaggtgcg cgacggcacc cccgcgccgg
     5581 ccgccgaggc cgcgtgcgtg gtgccggtgc gctaccggcg cctgctggtg cagcacgagc
     5641 aggtgcgcaa ggtcacgctc acgtggtggc agcgcttcct gcgcgcctcg ggccgcgggc
     5701 cggcgttcct gcggctggcg atcgccggcg gcatcgtcgg cgcggtgctg acggtcggcg
     5761 ccgacatcgg cagcgcgacg ctggtgatct acgacggcct ggacatcccc gtgcgggtgc
     5821 aggtgggcga gaccggccac gacgtcgagc ccggcagtcc gctgaagctg gacgtcgagc
     5881 ccgactcgaa gctggccatc gtgaccacga cgcgcgacgg ccgcgagatc gaacgcttcg
     5941 acgcgaagat ccagggcggc ttcggcacgc aggtctacaa cgtcgccggc gcgagcccgc
     6001 tggtgcgctg gacggcggtc tacggcccgg cgcgcgagga gccgcctcag ccgctgggag
     6061 cgccgcgcct ggtggccgtg tcggtcgacc acctgttcga ggatccgccg cgcagcgtcg
     6121 agacgcgcca tggcgagggc gcggtgcgcc acgtgctgca gggcttcgcc ggctcgacgc
     6181 cgcaggagct gctgggcgcg ctcggctcgg acgtggccac ggcgcatcac gtcgcgctgg
     6241 tccacgcgcg ctgggacgtc gaccaccagg gcgacctcga gagctggttc gccgccgcca
     6301 gcggcgagcc ggggctggcg gcggtcctcc aggatcgcct ggcgcgctcg cccgacagcg
     6361 tcgccctggc ccgcgcggcg cagtacctgg cgggcgaagg ccccgcgcac gaggccgcct
     6421 gcgcgcgcga ccgcgcgcgc gccgcgcaac gtcccgacga cggcgaccgc gcctatctgg
     6481 cgatccgctg cgagcccgac gagcggtcgg gcgccgactt catcgccgcg caccagcgct
     6541 ggccgaccaa cccgtggctc gccagcgccg ccggctgggc ctatgcgaac gacgagcgct
     6601 gggacga
//
LOCUS       DATCRO010000085         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_608621, whole genome shotgun sequence.
ACCESSION   DATCRO010000085 DATCRO010000000
VERSION     DATCRO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608621"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..992)
                     /locus_tag="VIN75_02285"
     CDS             complement(267..992)
                     /locus_tag="VIN75_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8973016.1"
                     /translation="MPASRRASPPPPDAPHEPRLARIAALVADPSRARMLAFLLSGEY
                     ASAGELARAASVGAATASAHLVKLVDAGLLACEPRGRHRYFRIADADVAHALEALAMV
                     AERSSHDRAWSAPARQRLREARCCYGHLAGRRGVALFDHLRRNAWLVDAPDGYALTEA
                     GVAGLAGLGFDAAALQASASHRIAYPCLDWSERRDHLAGKLASGLLDHFMERGWLRRI
                     GSERALELTPPGRQALAPLLAID"
     gene            complement(1019..1354)
                     /locus_tag="VIN75_02290"
     CDS             complement(1019..1354)
                     /locus_tag="VIN75_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY8973017.1"
                     /translation="MTLTCFIRYEIDPFKREHFRQYCEAWGRIIPRCGGHLLGYFLPS
                     EGTNYEAWGLISFDSLAAYEAYRKRLRADAQGRANFEFAQQERFIVREERTFTEVVDA
                     AYLRASEIQ"
     gene            complement(1433..2071)
                     /locus_tag="VIN75_02295"
     CDS             complement(1433..2071)
                     /locus_tag="VIN75_02295"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8973018.1"
                     /translation="MSDSGHDAITIQPSVLYFGTPVALVTTLDERGAANITPMSSIWA
                     LDDRLVLGLSGQGQGCMNLLHSGEAVVNLPDPPLHAAIERIARTTGRTPVPDDKQALR
                     YRHEADKFALGGFTRAASQLVAPPRIAQCPLQLETRLLHARPSTPGAGEDARDPGFLV
                     IELAVVRVHAHRAIVLDGTQHVDTARWSPLLYVFRHYYGTGERRGRNFRAET"
     gene            2299..>3079
                     /locus_tag="VIN75_02300"
     CDS             2299..>3079
                     /locus_tag="VIN75_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HEY8973019.1"
                     /translation="MTAARVSIDFAGAWRRAGAALAWLGGVALQLQQPALWDGAVNAL
                     LLAGGLASWLMARALRRAGRPRLAWLAACLAVAAAGAAYAGWRADLRLADALAPAWEG
                     CDIELVGTVDDMPQLAPDGEHFSFAVESARPLAMTPRELRRVRALADAAGQPLPGAVA
                     AAGAARPDAAAPGDDDDDDAPASSPPTDAGAPVVPSRVWLAWSRNQRDDRSVAEAPPP
                     LRAGQRWRLPVRLRRPHGAMNPDGFDAELWLFDQGLRATGSV"
BASE COUNT          384 a         1067 c         1238 g          390 t
ORIGIN      
        1 gggcggcgat ggtcatgtcg gttctccgtg caggggcggc gggccccgtg atgggcacgg
       61 atgctacacg cccgtccaat cacctgtcgc cgcgcgggcc gcgggcggcg gcgcggaggg
      121 gacgagcggc ggcgggcggg ccgcgaacgc ccgcgccgcg tgttggcagc tcaggtcatg
      181 acccgccagg caccggcgcg ccgagccgcc gatcgcttgg caggtcgggt caccacccac
      241 caggcaccgg cacgccgagg cgccgatcag tcgatggcca gcagcggcgc caaagcctgc
      301 cgccctggcg gcgtcagctc gagcgcgcgc tcgctgccga tgcggcgcag ccagccgcgc
      361 tccatgaagt ggtccagcag gccgctggcc agcttgccgg cgaggtggtc gcgccgctcc
      421 gaccagtcca ggcagggata ggcgatgcgg tgcgacgcgc ttgcctgcag cgcggcggca
      481 tcgaagccga gcccggccag cccggcgacg ccggcctcgg tcagcgcgta gccgtccggg
      541 gcgtccacca gccaggcgtt gcggcgcagg tggtcgaaca gcgcgacgcc gcgccggccc
      601 gccaggtggc cgtagcagca gcgcgcctcg cgcagccgct ggcgcgccgg cgccgaccag
      661 gcgcggtcgt gcgacgagcg ttcggccacc atcgccagcg cctccagcgc gtgcgccacg
      721 tcggcatcgg cgatgcggaa gtagcgatgg cgcccgcgcg gctcgcaagc gagcagtccc
      781 gcgtcgacca gcttgaccag gtgcgcgctg gccgtggccg cgcccaccga ggccgcgcgc
      841 gcgagctcgc cggcgctggc gtactcgccc gacagcagga acgcgagcat gcgggcacgc
      901 gacgggtcgg ccaccagcgc ggcgatgcgc gccagccgcg gctcgtgcgg ggcgtcgggg
      961 ggcggcgggc tggcgcggcg cgaagcgggc atgcggagaa ggtcaggctg cggatgcatc
     1021 actgtatttc agaagcgcgc aggtacgccg cgtcgaccac ctcggtgaag gtgcgctcct
     1081 cgcggacgat gaagcgctcc tgctgcgcga actcgaagtt ggcgcggccc tgggcgtcgg
     1141 cgcgcaggcg cttgcggtag gcctcgtagg cggcgaggct gtcgaacgag atgaggcccc
     1201 aggcctcgta gttggtgccc tcgctgggaa ggaagtagcc gagcaggtgg ccgccgcagc
     1261 gcgggatgat gcggccccag gcctcgcagt actggcggaa gtgctcgcgc ttgaacgggt
     1321 cgatctcgta gcggatgaag caggtcaggg tcatggtgtc tccgttggat gccgccagtc
     1381 tcgcgccggc cggggccgcg atgcttcgcc gcgcgacgaa acgtcggcgg cctcaggtct
     1441 cggcgcggaa gttgcgcccg cgccgctcgc ccgtgccgta gtagtggcgg aacacgtaca
     1501 gcagcgggct ccagcgcgcg gtgtcgacgt gctgcgtgcc gtccagcacg atggcccgat
     1561 gcgcgtggac gcggacgacg gcgagctcga tgaccaggaa acccgggtcg cgcgcgtcct
     1621 cgcccgcgcc cggcgtcgat ggtcgcgcgt gcagcagccg cgtctcgagc tgcagcgggc
     1681 actgggcgat gcgcggcggc gccaccaact gcgatgcggc gcgcgtgaag ccgccgagcg
     1741 cgaacttgtc ggcctcgtgg cggtagcgca gggcctgctt gtcgtcgggc accggcgtgc
     1801 ggcctgtcgt ccgggcgatg cgctcgatgg cggcgtgcag cggcgggtcg ggcaggttga
     1861 cgacggcctc gccgctgtgc agcaggttca tgcagccctg gccctggccg gacaagccca
     1921 ggacgagccg gtcgtcgagc gcccagatcg aggacatcgg cgtgatgttg gcggcgccgc
     1981 gttcgtcgag cgtggtgaca agcgcgacgg gcgtgccgaa gtagaggacg gaaggctgga
     2041 tggtgatggc gtcgtggccg gagtcggaca tggcgagttg tcgaaaggcg tggcctggca
     2101 gcgtaggcgg ggcgtgcagg acacgcttcg ttgcgtgtcg aagcaagcgg tcggcgaacg
     2161 cgaagggacg ccgcggacgc aggccgggca gcgctcgccg cgcgacggcg ccggcgatgg
     2221 gccgtgcccg gcattcgcgg atgaaacccc ggggtttgcc gcgggcggtt ttccagcgcg
     2281 ttcctagact tcgatcgcat gacagcggct cgcgtctcca tcgacttcgc cggcgcatgg
     2341 cggcgcgccg gcgcggcgct ggcgtggctg ggcggcgtgg cgctgcagtt gcagcagccg
     2401 gcgttgtggg acggcgcggt caacgccttg ctgctcgccg gcggactggc gagttggctc
     2461 atggcgcggg cactgcggcg cgccggccgg cctcggctcg catggctggc cgcctgcctg
     2521 gcggtcgcgg ccgccggcgc ggcgtatgcg ggctggcgcg cggacctgcg cctcgccgac
     2581 gcgctggcgc cggcgtggga gggctgcgac atcgagctcg tcggcacggt cgacgacatg
     2641 ccgcagctcg cgcccgacgg cgagcacttc tcgttcgcgg tggagtcggc gcgtccgctg
     2701 gcgatgacgc cgcgcgagct ccggcgcgtg cgcgcgctcg ccgacgcggc cggccagccg
     2761 ttgcccgggg cggtggcggc ggccggggcg gccaggccgg acgcggcagc gccgggcgac
     2821 gatgacgacg acgacgcgcc cgcatccagc ccaccgacgg acgcgggcgc gccagtcgtg
     2881 ccttcgcgcg tctggctcgc ctggagccgc aaccagcgcg acgaccgcag cgtggcggaa
     2941 gcgccgccgc cgctgcgcgc gggccagcgc tggcgcctgc cggttcgcct gcggcgcccg
     3001 cacggcgcga tgaacccgga cggcttcgac gccgagctgt ggctgttcga ccagggcctg
     3061 cgcgccaccg gttcggtgc
//
LOCUS       DATCRO010000086         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1092472, whole genome shotgun sequence.
ACCESSION   DATCRO010000086 DATCRO010000000
VERSION     DATCRO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092472"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VIN75_02305"
     CDS             <1..816
                     /locus_tag="VIN75_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase"
                     /protein_id="HEY8973020.1"
                     /translation="YMDVLTNSATDIVTALAPKPGADRQKLVASFDAALTRLQADTTL
                     SRGDRLGALFARVDLARIDQPKNTMHPKLPPALVKEVRDTAATTDREVTNAFERQAVI
                     PGTSQLLEEAGMWKESEALLKSSLAKSHSPYYLMSELGSNARKQGRTGEALQWYQQAW
                     EKSDGPATRLQWGSSYLKALVELAPQDARRIESTAQSIFAEAAGQANAFDQRSGRSLE
                     RVGASLQKWNAGGKHQAAVDHLSTQVQGLCAKLPPADPQHATCESVFKASAKA"
     gene            854..2083
                     /gene="purT"
                     /locus_tag="VIN75_02310"
     CDS             854..2083
                     /gene="purT"
                     /locus_tag="VIN75_02310"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY8973021.1"
                     /translation="MTSNTKPLGTPLSPAATRVMLLGSGELGKEVLIALQRLGVETIA
                     VDRYENAPGQQVAHHAHTITMSDPVALRALIEREKPDLVVPEIEALATAELQALESEG
                     VVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYVFCDSLEELQAAIDGKGGQPAIG
                     YPCIVKPVMSSSGKGQSKIDGPADVKTAWDYAMAGGRVSHGRVIVEGFIDFDYEITLL
                     TVRSLGANGAVQTSFCAPIGHVQVKGDYVESWQPMPMDAAALASAERIAKAVTDDLGG
                     QGVFGVELFVKGADVWFSEVSPRPHDTGMVTMISQWQSEFELHARAILGLPVDVSLKS
                     PGASAVIYGGVDAKALQFDGVADALRVPGTDVRLFGKPESFERRRMGVALAHDTDVER
                     ARIKAKEAAAKVKPKAA"
     gene            complement(2105..2713)
                     /gene="gstA"
                     /locus_tag="VIN75_02315"
     CDS             complement(2105..2713)
                     /gene="gstA"
                     /locus_tag="VIN75_02315"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY8973022.1"
                     /translation="MKLYYSPGACSLSPHIALREAGVKFDLVKTALQTHTLADGSDYY
                     QINAKGQVPLLELDDGARLTEGPVILQYIADHAPASGLAPAAGTMERYRVMEWLNFIT
                     SELHKGFSPMFNPKLAKEAKDVFAARLQERYRLVDARLEGNDYLMGKQFSVADAYLFT
                     VTNWAAGVGVDLTGLKNLDAFMARMKSRPAVQDAMKAEGLLK"
BASE COUNT          482 a         1011 c         1012 g          394 t
ORIGIN      
        1 tacatggacg tgctgaccaa cagcgccacc gacatcgtca ccgccctcgc gcccaagccg
       61 ggcgccgacc gccagaagct cgtcgcatcg ttcgatgccg ccctcacgcg gctgcaggcc
      121 gacaccacgt tgtcgcgcgg cgaccgcctc ggcgcgctgt tcgcgcgcgt cgacctggcg
      181 cgcatcgacc agcccaagaa cacgatgcac ccgaagctgc cgccggcgct cgtcaaggaa
      241 gtgcgcgaca ccgccgcgac caccgaccgc gaggtcacca acgcgttcga gcgccaggcc
      301 gtgatcccgg gcaccagcca gctgctcgag gaagcgggca tgtggaagga atcggaggcg
      361 ctgctcaagt ccagcctggc aaagagccac tcgccgtact acctcatgag cgaactcggt
      421 tcgaacgcgc gcaagcaggg ccgcacgggc gaggcgctgc agtggtacca gcaggcctgg
      481 gagaagagcg atggcccggc cacgcgcctg caatggggct cgagctacct gaaggcgctg
      541 gtcgagctcg cgccgcagga tgccaggcgc atcgagtcca ccgcgcagtc catcttcgcc
      601 gaggcggccg gccaggccaa cgcgttcgac cagcgcagcg gccgctcgct ggagcgcgtc
      661 ggcgcctcgc tgcagaagtg gaacgccggc ggcaagcacc aggccgccgt cgaccacctc
      721 agcacgcagg tgcagggcct gtgcgcgaag ctgccgcccg ccgatccgca gcacgccacc
      781 tgcgaaagcg tcttcaaggc gtccgccaag gcctgagtct gcaaccgttt ctccatccgc
      841 cccttcgaac tccatgacct ccaacaccaa gcccctcggc accccgctca gccctgccgc
      901 cacccgcgtg atgctgctgg gcagcggcga actcggcaag gaagtgctga tcgcactgca
      961 acgcctgggc gtcgagacga tcgccgtcga ccgctacgag aacgcccccg gccagcaggt
     1021 ggcgcaccac gcgcacacga tcaccatgag cgacccggtg gcgctgcgcg cgctcatcga
     1081 gcgcgagaag cccgacctcg tcgtgcccga gatcgaggcg ctggcgacgg cggagctgca
     1141 ggcgctggag tccgaaggcg tcgtgcgcgt cattcccacg gcgcgcgctg cgcggctgac
     1201 gatggaccgc gaaggcatcc gccgcctcgc ggccgagacg ctgggcctgc ccacgagccc
     1261 ctacgtgttc tgcgactcgc tggaggagct gcaggccgcg atcgacggca agggcggcca
     1321 gccggccatc ggctacccgt gcatcgtcaa gccggtgatg agcagctccg gcaagggcca
     1381 gagcaagatc gacggtccgg ccgacgtgaa gaccgcctgg gactacgcga tggccggcgg
     1441 ccgcgtcagc cacggccgcg tgatcgtcga gggcttcatc gacttcgact acgagatcac
     1501 gctgctgacg gtgcgctccc tgggcgccaa cggcgcggtg cagaccagct tctgcgcgcc
     1561 gatcggccac gtgcaggtca agggcgacta cgtcgagagc tggcagccga tgcccatgga
     1621 cgccgcggcg ctcgccagcg ccgagcgcat cgccaaggcc gtcaccgacg acctgggcgg
     1681 ccagggcgtg ttcggcgtcg agctgttcgt caagggcgcg gacgtgtggt tcagcgaggt
     1741 cagcccgcgc ccgcacgaca ccggcatggt cacgatgatc agccagtggc agagcgagtt
     1801 cgaactgcac gcccgcgcga tcctcggcct gccggtggac gtgtcgctca agagcccggg
     1861 cgccagcgcg gtgatctacg gcggcgtcga cgccaaggcg ctgcagttcg acggcgtggc
     1921 cgacgcgctg cgcgtgccgg gcaccgacgt gcgcctgttc ggcaagcccg agagcttcga
     1981 gcgccgccgc atgggcgtgg cgctcgcgca cgacacggac gtcgagcgcg cccgcatcaa
     2041 ggccaaggaa gccgcggcca aggtcaagcc gaaggcggcc tgacctcggg gacgcggcga
     2101 cgcgtcactt gagcaggcct tcggccttca tcgcgtcctg cacggcgggg cgcgacttca
     2161 tgcgggccat gaaggcgtcc aggttcttga ggccggtcag gtcgacgccg acgccggcag
     2221 cccagttcgt cacggtgaac aggtaggcgt ccgcgacgct gaactgcttg cccatgaggt
     2281 agtcgttgcc ctcgaggcgt gcgtcgacga gcctgtagcg ctcctgcagg cgcgcggcga
     2341 agacgtcctt ggcttccttc gcgagcttcg ggttgaacat cggagagaaa cccttgtgca
     2401 gttcgctggt gatgaagttc agccactcca tcacgcgata gcgctccatc gtgccggccg
     2461 ccggcgcgag gccgctggcc ggcgcatggt cggcgatgta ttgcaggatg accgggcctt
     2521 cggtgaggcg cgcgccgtcg tcgagctcga gcagcggcac ctggcccttg gcgttgatct
     2581 ggtagtagtc gctgccgtcg gcgagcgtgt gcgtctgcag cgcggtcttg acgaggtcga
     2641 acttcacgcc cgcctcgcgc agcgcgatgt ggggggacag cgagcaggcg ccggggctgt
     2701 aatagagctt catgacggtc tttcgtgagt gggaaacgcg ccggacgggg cggcgtggcc
     2761 gggcgcccat gatgcccaaa acgccgccat gttgcagggc agggcattcg acggaacgac
     2821 gcggaccgcc gtcgaacggc ggcggaggcg caaggcaggt ctgctacgct gcgggccagc
     2881 tcaacgacag gctccgcat
//
LOCUS       DATCRO010000087         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_225979, whole genome shotgun sequence.
ACCESSION   DATCRO010000087 DATCRO010000000
VERSION     DATCRO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225979"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VIN75_02320"
     CDS             complement(<1..391)
                     /locus_tag="VIN75_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8973023.1"
                     /translation="MMPPLAAAWRMLVRDWRAGELAVLAIALVIAVASVTSVGFFADR
                     VGRALERDAHQLLGADLVLVADHPWDKRIEARIVGAGAGHAEALTFVSMALKGEANQL
                     VGVKAVSEGFPLRGRLRIAAAPGARDEE"
     gene            complement(388..1062)
                     /locus_tag="VIN75_02325"
     CDS             complement(388..1062)
                     /locus_tag="VIN75_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8973024.1"
                     /translation="MSEPIIVVDRLTRQVSDATGTLTILHDISFTLNARESAAIVGAS
                     GSGKSTLLGLLAGLDVPTAGSVKLAGSDLFALDEDARAAVRAERVGFVFQSFQLLAHR
                     TALENVMLPLELAGARDARARARASEMLARVGLAERLGHYPRVLSGGEQQRVALARAF
                     VARPKLLLADEPTGSLDAASGEAVIRLLFEMNRENGTTLVLVTHDESLARRCGRSVRL
                     VAGRIA"
     gene            1061..1750
                     /locus_tag="VIN75_02330"
     CDS             1061..1750
                     /locus_tag="VIN75_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY8973025.1"
                     /translation="MHSLFGARPARRRFLHFSLSLAAALSLGLAATVHAASPSSAGDG
                     PVIVVVGDSISAEFGLARGSGWVALLGQRLAAAKSRWTVSNASISGDTTAGGLSRLPV
                     ILQQRHPKIVIIELGGNDALRGLPMSATRKNLDAMATLAEAAGARVLVAGMMVPPNFG
                     RKYQAEFGQMYADVAAARHAALVPFLFAGIADRPDADAWFQADRIHPLAKAHPIILDN
                     VWAKLKPMLGS"
     gene            complement(1926..3362)
                     /locus_tag="VIN75_02335"
     CDS             complement(1926..3362)
                     /locus_tag="VIN75_02335"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY8973026.1"
                     /translation="MDTSHALVHPVIPSAHRPWRRRALAAALALGCLASAAVRAQEDP
                     PGRVGRVADLAGDVRTLNADGGWMDVVRNQVLSTGDRITTDKTGRATLQIGSTVVRLG
                     ADSDLVVTQMDDQHVRLRFDHGQLAVRVRSEDIPGELFIDTDEGAWVPHHAGQYRFDR
                     VPRQPLGAQAWSGDMLLEAPDSSLPLKAPQRALVWRDARNATHYHLDPAVKDAFGDWV
                     LAQDKQDDAHAAANTAAQVSPEMTGEADLARFGAWSAGPGGARVWTPAKLPAGWQPYQ
                     QGAWSWKLPWGWTWVDDQPWGFAPFHYGRWLQLKGRWAWTPGQWGSARPVFAPALVGW
                     LGGPALSMDGGPAIGWVALAPDEAVFPGYAVSAKYWNAITEPSVAAASRRPVAINGRA
                     RLVPAGPASYANAKVPGAVTVVGASALAPWVPVSQMPGPRGAAQRKPAFEKALAAAAP
                     TAPPGPPAGRGTLSASAPTLQVPLGPAR"
     gene            complement(3448..>3949)
                     /gene="def"
                     /locus_tag="VIN75_02340"
     CDS             complement(3448..>3949)
                     /gene="def"
                     /locus_tag="VIN75_02340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8973027.1"
                     /translation="RLLRVAKPVEQFDTPEMRELIADMFETMKHANGAGLAAPQIGVD
                     LQLVIYGYDNNPRYPDAPPVPRTVLINPVITPLGDAEVDGWEGCLSVPGLRGVVPRWE
                     RIRYTGVDEHGNAIDREADGFHARVVQHECDHLIGRLYPTRMRDFTQFGFTSVLFPDM
                     DPNADD"
BASE COUNT          515 a         1474 c         1435 g          525 t
ORIGIN      
        1 cctcctcgtc ccgggcgccc ggcgcggcgg cgatgcgcag gcggccgcgc agcggaaagc
       61 cttcgctcac cgccttgacc ccgacaagct ggttcgcctc gcccttgagg gccatgctga
      121 cgaacgtgag cgcttcggca tggccggcgc cggcgccgac gatccgagcc tcgatccgct
      181 tgtcccacgg atggtcggcg accagtacca ggtcggcgcc gagcagctgg tgcgcgtcgc
      241 gctccagcgc gcggccgacc cggtcggcga agaagccgac gctcgtcacg ctcgccacgg
      301 cgatgaccag cgcgattgcg agcaccgcga gctcgccggc gcgccagtcg cgcaccagca
      361 tgcgccaggc cgccgcgagc ggcggcatca tgcgatgcgc ccggccacca ggcgcacgct
      421 gcggccgcag cggcgcgcga gcgactcgtc gtgcgtgacc agcacgagcg tcgtgccgtt
      481 ttcgcggttc atctcgaaaa gcagccggat cacggcctcg cccgacgccg cgtcgaggct
      541 gccggtcggc tcgtcggcca ggagcagctt cggccgggcg acgaaggcgc gcgccagcgc
      601 gacgcgctgc tgctcgccgc ccgacagcac gcgcggatag tggcccagcc gctcggccag
      661 gccgacgcgg gccagcatct cgctggcgcg cgcgcgcgcg cgcgcgtcac gcgcgcccgc
      721 gagctcgagc ggcagcatca cgttctccag cgccgtgcga tgcgccagca actggaagct
      781 ctggaacacg aagcccacgc gctcggcgcg cacggccgcg cgggcatcct cgtcgagcgc
      841 gaacaggtcg ctgccggcca gcttgacgga tccggcggtg ggaacgtcca ggcccgcgag
      901 cagtccaagc agcgtgctct tgcccgagcc ggaggcgccg acgatggcgg ccgactcgcg
      961 cgcgttcagc gtgaacgaga tgtcatggag aatcgtcagg gttccggtgg cgtcgctcac
     1021 ctggcgggtg agccgatcga cgacgatgat gggttccgac atgcattcac ttttcggggc
     1081 gcggccagcg cgccgcagat tcctgcactt tagcctgagc ctcgcggcgg cgctgtcgct
     1141 cggcttggcg gccacggtcc atgcggcctc accgtcctcc gcgggcgacg ggcccgtgat
     1201 cgtcgtcgtg ggcgacagca tctccgccga gttcggcctg gcgcgcggca gcggctgggt
     1261 cgccctgctg ggccagcgcc tggccgcggc caagtcccgc tggacggtgt ccaacgcgag
     1321 catcagcggc gacacgaccg cgggcggact ctcgcgcctg cccgtgatcc tgcagcagcg
     1381 ccatccgaag atcgtgatca tcgagctcgg cggcaacgac gcgctgcgcg gcctgcccat
     1441 gagcgccacg cgcaagaacc tcgatgcgat ggcgacgctg gccgaggccg ccggcgcgcg
     1501 tgtgctggtc gcgggcatga tggtgccgcc caacttcggc cgcaagtacc aggccgagtt
     1561 cgggcagatg tacgcggacg tcgccgccgc gcgccacgcc gcgctcgtgc ccttcctgtt
     1621 cgccggcatc gccgaccgcc ccgacgcgga cgcctggttc caggccgacc ggatccatcc
     1681 gctcgccaag gcgcatccga tcatcctgga caacgtctgg gcgaagctga agccgatgct
     1741 cgggtcgtag ggcgggttag ccgcaggcgt aacccgccgt tcgccggcgt cccgacccgc
     1801 cgttcgccgg cgtcccgatc cgccgttcgc cggcatcctg actcgccatt cgcccgcgtc
     1861 ctgcttggcc gccggatggc gggttacgcc tgcggctaac ccgccccacg acccgccagg
     1921 ccgcttcacc gggccgggcc gagtggcacc tgcaacgtcg gcgccgacgc agacaacgtg
     1981 ccgcggcccg cgggtggccc cgggggcgcc gtcggcgcgg ccgccgccag cgccttctcg
     2041 aacgccggct tgcgctgtgc agcgccgcgc ggcccgggca tctgcgacac cggcacccac
     2101 ggcgcgagcg cgctcgcgcc caccaccgtc accgcgcccg gcaccttggc gttcgcgtag
     2161 ctggcggggc ccgcgggcac gagccgcgcg cggccgttga tcgccacggg gcgacggctg
     2221 gccgccgcca cgctcggttc ggtgatcgcg ttccagtact tggccgacac tgcgtagccc
     2281 gggaacaccg cctcgtccgg cgccagcgcc acccagccga tggccgggcc gccgtccatg
     2341 ctcaacgcgg ggccgccgag ccatccgacc agcgccggcg cgaagacggg ccgcgcgctg
     2401 ccccactgcc ctggcgtcca cgcccagcgg cccttgagct gcagccagcg tccgtagtgg
     2461 aacggcgcga agccccaggg ctggtcgtcg acccaggtcc agccccaggg cagcttccac
     2521 gaccacgcgc cctgctggta aggctgccag ccggcgggca acttcgcggg cgtccacacg
     2581 cgcgcgccgc ccgggcccgc gctccacgca ccgaagcgcg cgagatcggc ctcgccggtc
     2641 atctcgggcg acacctgagc cgcggtgttg gcggctgcat gcgcgtcgtc ctgcttgtcc
     2701 tgcgccagca cccagtcgcc gaacgcgtcc ttgacggccg ggtccaggtg atagtgcgtg
     2761 gcgttcctgg cgtcgcgcca caccagcgcg cgctgcggcg ccttgagcgg cagcgagctg
     2821 tcgggcgcct cgagcagcat gtcgccgctc caggcctgcg cgccgagggg ctggcgcggc
     2881 acgcggtcga agcggtactg gccggcgtgg tgcggcaccc aggcgccctc gtcggtgtcg
     2941 atgaacaact cgcccgggat gtcctcggag cgcacgcgca ccgcgagctg gccgtggtcg
     3001 aaacgcaggc gcacgtgctg gtcgtccatc tgcgtgacga cgaggtcgga gtcggcgccc
     3061 aggcgcacga cggtcgaccc gatctgcagc gtcgcgcggc ccgtcttgtc ggtggtgatg
     3121 cgatcgccgg tggacagcac ctggttgcgc acgacgtcca tccagccgcc gtcggcgttg
     3181 agggtgcgca cgtcgcccgc gaggtcggcg acccggccga cgcggcccgg cggatcctcc
     3241 tgcgcgcgca cggcggcgct ggcgaggcag ccgagcgcga gcgccgcggc cagcgcgcgc
     3301 cggcgccagg gccggtgggc ggacgggatg accggatgaa cgagcgcgtg cgaggtgtcc
     3361 atgatcattc aacgcgagac ggggggcgcc ggccgaccgg cgtgcgtcgc gccatggccg
     3421 agcaatcggc gtgcgcgcgc ggggatctca gtcgtccgcg ttcgggtcca tgtccgggaa
     3481 cagcacgctg gtgaagccga actgcgtgaa gtcgcgcatg cgcgtcgggt acaaccggcc
     3541 gatcaggtgg tcgcactcgt gctgcacgac gcgggcatgg aagccgtcgg cctcgcggtc
     3601 gatcgcgttg ccgtgctcgt cgacgcccgt gtagcggatg cgctcccagc gcggcacgac
     3661 gccgcgcagg cccggcaccg agaggcagcc ctcccagccg tcgacctcgg cgtcgcccag
     3721 cggcgtgatc accgggttga tcagcaccgt cctgggcacc ggcggggcgt cggggtagcg
     3781 cgggttgttg tcgtagccgt agatgaccag ctgcaggtcg acgccgatct gcggcgcggc
     3841 caggccggcg ccgttggcgt gcttcatcgt ctcgaacatg tcggcgatga gctcgcgcat
     3901 ctcgggggtg tcgaactgct cgacgggctt ggcgacgcgc aggaggcgc
//
LOCUS       DATCRO010000088         9083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_57240, whole genome shotgun sequence.
ACCESSION   DATCRO010000088 DATCRO010000000
VERSION     DATCRO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57240"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..163
                     /locus_tag="VIN75_02345"
     CDS             17..163
                     /locus_tag="VIN75_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973028.1"
                     /translation="MSTFSQSSPLKRLVEPDEVAATVLWLCTDAPAAFTGQAMSVSGG
                     EVMN"
     gene            417..977
                     /locus_tag="VIN75_02350"
     CDS             417..977
                     /locus_tag="VIN75_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8973029.1"
                     /translation="MKGKATRDDTTDRLDELLGDDAIGHEGRAATTNHAAVKTWLRLL
                     SCTTQIETEIRRRLRLHFDMTLPRFDYLAQLHRFHQEQPEGLRMSALSRYLMVTGGNV
                     TSLTDELEKSGYVTRQVDPEDRRSTRIALTTRGKRAFERVAEEHERWVIELLAGLDAG
                     DGEQLHALLGRLRLSLTRRLQDDKTR"
     gene            1003..1857
                     /locus_tag="VIN75_02355"
     CDS             1003..1857
                     /locus_tag="VIN75_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY8973030.1"
                     /translation="MTTTPTDPALLAGNRRPLADYRATHFLWECSDRVARITLNRPER
                     KNPLSFESYAELRDLFIRLRHASDVRAIVISGAGENFCSGGDVHEIIGPLIDLPAPEL
                     LMFTRMTGDLVKAMRHCPQPIVAAVDGVCAGAGAILAMASDLRYGTTRSKTAFLFARV
                     GLAGCDMGACAMLPRIVGQGRAAEWIYSGRALGGEEAERWGFFNRLVEPDALLAEAGA
                     FARQLADGPAFAHSLTKSMLHQEWSMDIDTAIDAEATAQALCMLTEDFGTAYRAFAAK
                     SRPVFAGR"
     gene            1859..3085
                     /locus_tag="VIN75_02360"
     CDS             1859..3085
                     /locus_tag="VIN75_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8973031.1"
                     /translation="MLPHAMENVAAAAAQALEWPFFDQTHRTLHARLAASLADGASGF
                     PHELSPQATRDEVDAQCRRWVRALGNAGWLKLAVGGTAHGGASDALDTRTLCLMRETL
                     AAHDGLADFAFAMQGLGSGAITLDGSPELKARYLPRVARGEAIAAFALSEPEAGSDVA
                     AMRMTARRDGAHWVLDGQKTWISNGGIADFYVVFARQAEPGAAPEARATKSIAAFVVD
                     AGTPGLRIAERLDVMAPHPLARLSFEGCRVPASHCLAEAGGGFKLAMRTLDVFRTSVA
                     AAALGFARRAMAEGLARATSRPMFGQQLADLQLTQARLADMALTVDSAALLTYRAAWR
                     RDQGHTVTREAAMAKLAATEGAQRVIDAAVQLWGGAGVQSGNTVERLYRDIRALRIYE
                     GASEVQQLIIARELLK"
     gene            3082..4725
                     /locus_tag="VIN75_02365"
     CDS             3082..4725
                     /locus_tag="VIN75_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8973032.1"
                     /translation="MSIPSRGRTAHVDTFTRDHLPPLAQQPVYLFDLPELQFPPQLNC
                     AAEILDAAIARGWGGRPCIVGPGVRWTYADLQRQADAIAHVLVQEMGVRPGQRVLLRA
                     PNNPMLAACWFGVMKTGAVAVATMPLLRAKELKQVVAKAQVTHALCDRQLAEELQLAQ
                     PHCPTLTQVRLFNDASPDSLEAAMARHPTPFEAVDTAADDTCLLAFTSGTTGEPKATM
                     HFHQDVMAICLCWPRHVLRASADDVFIGSPPLGFTFGLGGLLLFPLSIGASTVLLEKA
                     TPPLLLDAIPAYGATVLFTAPTTYRAIADQARERKLSAPLGGTLRKCVSAGEALPAAT
                     RVQWKAATGLEAIDGIGSTEMLHIFISADEAHARPGATGTPVPGYQACILDPDGRPLP
                     RGAVGRLAVRGPTGCRYLDDPRQAQYVHDGWNVTGDAYLEDEDGYFRYQARTDDMIIS
                     AGYNIAAPEVETVLLEHAAVAECAVIGVPDEERGQIVKAFVVLRAGHAPDAATVKALQ
                     DYVKRSVAPYKYPRAIEFVPDLPRTATGKLQRFRLKTTP"
     gene            4763..5167
                     /locus_tag="VIN75_02370"
     CDS             4763..5167
                     /locus_tag="VIN75_02370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8973033.1"
                     /translation="MKNVLLPDGWPRPRGYANGVTAQGRQVYVAGMVGWNAASQFETD
                     DLVEQVRRALYNIKAVLDTAGARPDHMVRMTWYVIDRAEYLARGREIGLAYREVMGPN
                     YDVAMSAVEVSALMEARAKVEIEVTAVVPQSF"
     gene            complement(5176..5733)
                     /locus_tag="VIN75_02375"
     CDS             complement(5176..5733)
                     /locus_tag="VIN75_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEY8973034.1"
                     /translation="MSQTPRHALRLVPFAAVALLAACHTPALKPTPPSVVVPGNLRPP
                     ANESLVRTLWADGVQVYECRKAADASFPEWVLVGPEAKLADAAGTPMGRHYAGPTWEA
                     SDGSKVVGVVSARVDAPDPRAIPWLLLTTHSTGKSGIFAKVTSIQRVATTGGLAPTTG
                     CGTATVGKQERVPYTAQYAQYAPTL"
     gene            complement(5761..6627)
                     /locus_tag="VIN75_02380"
     CDS             complement(5761..6627)
                     /locus_tag="VIN75_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEY8973035.1"
                     /translation="MSYILDALRRADAERSRGAVPGLHAQGLPADVEPAARDYRPLIW
                     AAGGAGVLLVAGVVILLVAPWKSVQVPPESRAALAPTPDLSPTPRDAAAAFAPVQMAQ
                     GELPAAQPGAIEPGARPVEPPIGRGAAPQVAPAPAPAPQPYPPTAQAVRAAQRDTLAD
                     RAAEGRVATLEPRAAARATAAPPSPVPAQAPVEHYGPAIAPGTAPATAAARSNAVPNI
                     KDLPPDVRSQLPKLAVAGSVYSETPSARMVIFNGQVFHEGDKLGPDTVLEQIRLKSAI
                     LNFRGQRYEITF"
     gene            complement(6632..8359)
                     /locus_tag="VIN75_02385"
     CDS             complement(6632..8359)
                     /locus_tag="VIN75_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8973036.1"
                     /translation="MYAPFFGLKQKPFSIAPDPRYLFMSERHREALAHLLYGVQGGGG
                     FVLLTGEIGAGKTTVCRCFLEQIPARCNVAYIFNPKLSALELLVTVCHEFGIKFPPLT
                     AAGTPPGVKDYVDGLNEYLLRTHAVGQNNVLIIDEAQNLSSDVLEQLRLLTNLETSER
                     KLLQIVLIGQPELRVMLARPELEQLAQRVIARYHLDALTEKETAQYIAHRLAVAGLKG
                     ALPFDGGALRRIHQLTRGIPRRINLLCDRALLGAYATGKSRVDRTIVDKAAREVFGPH
                     DSHGEPARASAPRWWPLAAAGGALVGAAAMAAVHWGGREKAPASLVAAVAASAASAAQ
                     PRASLPAAVASTLPTAASAAAATPRLRSIDDLESLLARAPRDEHEALRTLAPEWSLKL
                     GDGDPCALAAEQDVRCFRAHGGLALLRLLARPSVLALRDAQDRQVYVVLAGLSHDEAT
                     LVVGKSPLLVPIPLLTTAWRGDFTTLWRVPPGYSVPLSMGSHGALVDAVGAQLAARQG
                     AAPPPADHAFDATLRARVSAFQLAEGLTPDGVAGPTTLMQINRASGVSEPWLAGTAPA
                     TAAAAAQKR"
     gene            8773..>9083
                     /locus_tag="VIN75_02390"
     CDS             8773..>9083
                     /locus_tag="VIN75_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8973037.1"
                     /translation="MRIQLASDLHLELLASTWPQERLVAPAPGADVLVLAGDIDRGLR
                     TIERFADWPVPVLYVAGNHEFYDHEWEQLRDDLRDRAAGVVDARGAGLDVLGGLLDQR
                     A"
BASE COUNT         1261 a         3314 c         3313 g         1195 t
ORIGIN      
        1 cagccaggcc gatgcgatgt cgacattctc gcagtccagc ccgctcaagc gcctggtcga
       61 acccgacgag gtggccgcga ccgtcctgtg gctgtgcacc gacgcgccgg cggcgttcac
      121 ggggcaggcg atgtcggtca gtggtgggga ggtgatgaat tgacttcgac agacggccgc
      181 aggaacgtcg ccgcaggggt ctggcctctc ctcggatcgc gccatgccct tcccgccggc
      241 cgcgccatcg atcgcgaatc gaggacaggc ctgaccccgt ctgcgcgaac gatcgaggtc
      301 aggcctgtcc ttcatccgcg aagtggcccc gccgccttcg ggcaccgtcg tcgcgcggat
      361 tcaggagagg ccagacccct ccggcgtcgc ggcgcccccg cacgcaggga ggtctcgtga
      421 aaggcaaggc gacgcgcgac gacaccaccg atcgcctcga cgagctcctc ggcgacgacg
      481 ccatcggcca cgaaggccgc gccgcgacga ccaaccacgc cgccgtcaag acctggctgc
      541 gcctgttgtc ctgcaccacg cagatcgaga ccgagatccg gcggcgcctg cggctgcact
      601 tcgacatgac gctgccgcgc ttcgactacc tcgcccagct gcaccgcttc caccaggaac
      661 agcccgaagg cctgcgcatg agcgcgctgt cgcgctacct catggtgacc ggcggcaacg
      721 tcaccagcct gaccgacgaa ctcgagaaga gcggctacgt cacgcgccag gtcgatccgg
      781 aagaccggcg ctcgacgcgc atcgcgctca ccacgcgcgg caagcgcgcg ttcgagcgcg
      841 tggccgagga gcacgagcgc tgggtgatcg agctgctcgc gggcctggac gccggcgacg
      901 gcgagcagct gcacgcgttg ctgggccgcc tgcggctgtc gctcacgcgc cggctgcagg
      961 acgacaagac ccgttgaaca tcggaaggac agggcacctg aaatgaccac cacccccacc
     1021 gatcccgcgc tgctggccgg caaccgccgc ccgctggccg actaccgcgc cacgcacttc
     1081 ctgtgggagt gcagcgaccg cgtcgcgcgc atcacgctga accggcccga gcgcaagaac
     1141 ccgctgagct tcgagagcta cgccgagctg cgcgacctgt tcatccgcct gcgccacgcg
     1201 agcgacgtgc gcgccatcgt catcagcggc gccggcgaga acttctgctc cggcggcgac
     1261 gtgcacgaga tcatcggccc gctgatcgac ctgcccgcgc ccgagctgct gatgttcacg
     1321 cgcatgaccg gcgacctggt caaggcgatg cgccactgcc cgcagcccat cgtggcggcg
     1381 gtcgacggcg tgtgcgccgg cgccggcgcg atcctcgcga tggccagcga cctgcgctac
     1441 ggcaccacgc gctcgaagac ggccttcctg ttcgcccgcg tcgggctggc cggttgcgac
     1501 atgggcgcgt gcgcgatgct gccgcgcatc gtcggccagg gccgcgccgc ggaatggatc
     1561 tactcgggcc gcgcgctcgg cggcgaggag gccgagcgct ggggcttctt caaccggctc
     1621 gtcgagcccg acgcgctgct cgccgaggcc ggcgcgttcg cgcgccagct cgccgacggc
     1681 ccggcgttcg cgcactcgtt gacgaaatcc atgctgcacc aggaatggag catggacatc
     1741 gacaccgcga tcgacgccga ggccaccgcg caggcgctgt gcatgctcac cgaggacttc
     1801 ggcaccgcgt accgcgcgtt cgccgcgaag tcccggccgg tcttcgcggg gcgctgagat
     1861 gctgccgcac gccatggaga acgtcgccgc ggcggccgcg caggccctcg agtggccgtt
     1921 cttcgaccag acccatcgca cgctgcacgc gcgcctggcc gcgagcctgg ccgacggcgc
     1981 gagcggcttc ccgcacgagc tgtcgccgca ggccacgcgc gacgaggtcg acgcgcaatg
     2041 ccgccgctgg gtgcgcgcgc tcgggaacgc cggctggctc aagctcgcgg tcggcggcac
     2101 ggcccacggc ggcgcgagcg acgcgctgga cacccgcacg ctgtgcctga tgcgcgagac
     2161 gctcgccgcg cacgacggcc tggccgactt cgcgttcgcg atgcagggcc tgggcagcgg
     2221 cgccatcacg ctcgacggct cgccggagct gaaggcgcgc tacctgccgc gcgtggcgcg
     2281 cggcgaggcg atcgcggcgt tcgcgctgtc cgagcccgag gccggctccg acgtcgccgc
     2341 gatgcgcatg accgcgcggc gcgacggcgc gcactgggtt ctggacggcc agaagacgtg
     2401 gatctccaac ggcggcatcg ccgacttcta cgtggtgttc gcacgccagg ccgagcccgg
     2461 cgcggcgccc gaggcgcgcg ccacgaagtc gatcgcggcc ttcgtcgtcg atgccggcac
     2521 accgggcctg cgcatcgccg agcgcctgga cgtgatggcg ccgcatccgc tcgcgcggct
     2581 gtcgttcgaa ggctgccgcg tgccggcgtc gcactgcctg gccgaggccg gcggcgggtt
     2641 caagctggcg atgcgcacgc tggacgtgtt ccgcacgtcc gtcgccgcgg cggcgctcgg
     2701 cttcgcgcgc cgcgccatgg cagaaggcct cgcgcgcgcg acctcgcgcc cgatgttcgg
     2761 ccagcagctc gccgacctgc agctgacgca ggccaggctg gccgacatgg cgctcaccgt
     2821 cgattcggcc gccctgctca cgtatcgcgc cgcgtggcgc cgcgaccagg gacacaccgt
     2881 gacgcgcgag gccgccatgg ccaagctcgc cgccaccgag ggcgcgcagc gcgtcatcga
     2941 tgccgccgtg cagctgtggg gcggcgcggg cgtgcagagc gggaacaccg tcgaacgtct
     3001 ctaccgagac atccgtgcgc tgcgcatcta cgagggcgcc agcgaagtgc agcaactgat
     3061 catcgcccgg gagctgttga agtgagcatt ccatcgaggg gccggacggc ccatgtcgac
     3121 acgttcaccc gcgaccacct gccgcccctc gcgcagcaac cggtctacct gttcgacctg
     3181 cccgagctgc agttcccgcc gcagctcaat tgcgccgccg agatcctcga cgccgcgatc
     3241 gcgcgcggct ggggcgggcg gccctgcatc gtcgggcccg gcgtgcgctg gacctacgcg
     3301 gacctgcaac gccaggccga cgcgatcgcg cacgtgctgg tccaggagat gggcgtgcgg
     3361 cccggccagc gcgtgctgct gcgcgcgccg aacaacccga tgctcgccgc ctgctggttc
     3421 ggcgtgatga agacgggcgc cgtggcggtg gcgacgatgc cgctgctgcg cgccaaggaa
     3481 ttgaagcagg tcgtcgcgaa ggcgcaggtc acgcatgcac tgtgcgatcg ccagctggcc
     3541 gaggagctgc agctggcgca gccgcactgc ccgacgctga cgcaggtccg gctgttcaac
     3601 gacgcctcgc cggacagcct cgaggccgcg atggcgcgcc atccgacgcc gttcgaggcg
     3661 gtcgacaccg cggccgacga cacctgcctg ctcgcgttca cctccggcac caccggcgag
     3721 ccgaaggcga cgatgcactt ccaccaggac gtgatggcga tctgcctgtg ctggcccagg
     3781 cacgtgctgc gcgccagcgc cgacgacgtg ttcatcggca gtccgccgct gggcttcacg
     3841 ttcggcctgg gcggcctgct gctgttcccg ctgtccatcg gcgccagcac cgtgctgctg
     3901 gagaaggcga cgccgccgct gctgctcgat gccatccccg cgtatggcgc gacggtgctg
     3961 ttcacggcgc ccacgacgta ccgcgcgatc gccgaccagg cgcgcgagcg caagctgtcg
     4021 gcgccgctcg gcggcacgct gcgcaagtgc gtgtcggccg gcgaggcgct gcccgcggcc
     4081 acgcgcgtgc agtggaaggc cgccaccggc ctggaggcga tcgacggcat cggctcgacc
     4141 gagatgctgc acatcttcat ctcggccgac gaggcgcacg cgcgccccgg cgccaccggc
     4201 acgccggtgc ccggctacca ggcctgcatc ctcgaccccg acgggcgccc gctgccgcgc
     4261 ggagcggtcg gccggctggc ggtgcgcggc cccacgggct gccgctacct ggacgacccg
     4321 cgccaggcgc agtacgtgca cgacggctgg aacgtcaccg gcgacgccta cctggaagac
     4381 gaggacggct acttccgcta ccaggcgcgc accgatgaca tgatcatctc ggccggctac
     4441 aacatcgccg cgcccgaggt ggagacggtg ctgctggagc acgcggccgt cgccgagtgc
     4501 gcggtgatcg gcgtgcccga cgaggagcgc ggccagatcg tcaaggcctt cgtcgtgctg
     4561 cgcgcagggc acgcgccgga cgccgcgacg gtcaaggcgc tgcaggacta cgtcaagcgc
     4621 tcggtcgccc cctacaagta cccgcgcgcc atcgagttcg tgcccgacct gccgcgcacc
     4681 gccacgggca agctgcagcg gttccgcctg aagacgaccc catgactttc gaccaatcga
     4741 cgacagaccc cgcgaggacg cgatgaagaa cgtgcttcta cccgacggct ggccgcgacc
     4801 gcgcggctac gcgaacggcg tgaccgccca gggccgccag gtctacgtgg ccggcatggt
     4861 cggctggaac gccgcttccc agttcgagac cgacgacctg gtcgagcagg tgcgccgcgc
     4921 gctctacaac atcaaggccg tgctcgacac cgccggcgcg cgccccgacc acatggtgcg
     4981 catgacctgg tacgtgatcg accgcgccga gtacctggcc cgcggccgcg agatcggcct
     5041 cgcctaccgc gaggtgatgg gcccgaacta cgacgtcgcg atgagcgccg tcgaggtcag
     5101 cgcgctgatg gaggcccgcg ccaaggtgga gatcgaggtg acggcggtgg tgccgcagtc
     5161 gttctgaggg cgcggctaca gcgtcggcgc gtactgagcg tactgcgccg tgtagggcac
     5221 gcgctcctgc ttgccgaccg tcgcggtgcc gcagcccgtg gtcggcgcga ggccgccggt
     5281 ggtggccacg cgctggatcg acgtcacctt cgcgaagatg ccggacttgc cggtgctgtg
     5341 cgtcgtcagc agcagccacg ggatcgcgcg cggatcgggg gcgtcgaccc tggccgagac
     5401 gacgcccacg accttgctgc cgtcgctggc ctcccacgtc gggcccgcgt agtggcggcc
     5461 catgggcgtg ccggcggcgt cggccagttt cgcttcgggt ccgacgagca cccactcggg
     5521 gaagctcgcg tcggccgcct tgcggcactc gtagacctgc acgccgtcgg cccacagcgt
     5581 acgcaccagc gactcgttgg cgggcgggcg caggttgccg ggaacgacca cggacggcgg
     5641 cgtcggcttg agcgccgggg tgtggcaggc cgccagcagc gcgacggcgg cgaacgggac
     5701 gaggcggagg gcgtggcggg gcgtctggct catgcggaac actttcggga aggaatcgga
     5761 tcagaacgtg atctcgtagc gctggccacg gaagttcagg atggcggatt tcaggcggat
     5821 ctgctcgagc acggtgtccg gcccgagctt gtcgccttcg tggaagacct ggccgttgaa
     5881 gatcaccatg cgcgccgacg gcgtctcgga atagacggag ccggccacgg cgagcttggg
     5941 cagttgcgaa cggacgtcgg ggggcaggtc cttgatgttc ggcacggcat tgctgcgcgc
     6001 ggcggctgtt gctggcgcgg tgccgggcgc gatggccggc ccgtagtgtt cgaccggcgc
     6061 ctgcgccggg acgggcgatg gcggggccgc ggtcgcgcgc gccgcggcgc gcggctccag
     6121 cgtcgcgacg cggccctcgg cggcgcggtc ggcgagcgtg tcgcgctggg cggcgcgcac
     6181 ggcctgcgcg gtcggcggat acggctgcgg cgcgggggct ggcgcgggcg cgacctgcgg
     6241 cgcagcgccg cgtccgatcg gcggctcgac cggccgcgcg cccggctcga tcgcgccagg
     6301 ctgcgccgcg ggcagctcgc cctgggccat ctgcaccggc gcgaaagccg cggcggcatc
     6361 gcgcggggtt ggcgacaggt cgggcgtcgg ggccagcgcc gcgcgcgact ccggcggcac
     6421 ctgcaccgac ttccacggcg cgaccagcag gatcaccacg cccgccacca gcagcacccc
     6481 ggcgccgccg gcggcccaga tcagcgggcg atagtcgcgc gccgccggct ccacgtcggc
     6541 cggcagcccc tgcgcgtgca ggcccggcac ggcgccgcgc gaacgttcgg cgtcggcgcg
     6601 ccgcagcgca tcgaggatgt aggacatgcg cttacctttt ctgggcggcg gccgccgccg
     6661 tcgcgggcgc cgtgccggcc agccagggct cggacacgcc gctcgcgcga ttgatctgca
     6721 tcagcgtcgt cgggccggcg acgccgtcgg gcgtcagtcc ttccgccagc tggaacgccg
     6781 agacgcgggc gcgcaacgtg gcgtcgaacg cgtggtcggc cggcggcggt gcggcgccct
     6841 ggcgcgcggc aagctgcgcg cccaccgcgt cgacgagcgc gccatgcgag cccatggaca
     6901 gcggcaccga gtagccgggc ggcacgcgcc agagcgtggt gaagtcgccg cgccaggcgg
     6961 tggtcagcaa cgggatcggc accagcagcg gcgacttgcc caccaccagg gtggcctcgt
     7021 cgtggctcag gccggccagc acgacgtaga cctgacggtc ctgcgcgtcg cgcagcgcga
     7081 gcacgctcgg gcgcgcgagc agccgcagca gcgcgaggcc gccgtgggcg cggaagcagc
     7141 gcacgtcctg ctcggccgcg agcgcgcacg gatcgccgtc gcccagcttg agcgaccact
     7201 cgggcgcgag cgtgcgcagc gcctcgtgct cgtcgcgcgg ggcgcgcgcg agcaggctct
     7261 ccaggtcgtc gatggagcgc aggcgcggcg tcgcggcggc ggcggaggcc gcggtcggaa
     7321 gcgtcgaggc gaccgccgcc ggcagcgacg cgcgcggttg cgccgccgat gcggcgctcg
     7381 cggccaccgc ggccacgagc gacgcgggcg ccttctcgcg gccgccccag tgcaccgccg
     7441 ccatcgcggc cgcgcccacc agcgcgccac cggcggcggc cagcggccac cagcgcggcg
     7501 ccgaggccct ggccggttcg ccgtgcgaat cgtgcgggcc gaacacctcg cgcgccgcct
     7561 tgtcgacgat cgtgcgatcg acgcgcgact tgcccgtggc atacgcgccc agcagcgcgc
     7621 ggtcgcacag caggttgatg cggcgcggga tgccgcgcgt gagctggtgg atgcgccgca
     7681 gggcaccgcc gtcgaacggc agcgcgccct tcaggccggc caccgcgagc cggtgcgcga
     7741 tgtactgcgc cgtctccttc tcggtgagcg cgtcgaggtg gtagcgcgcg atcacgcgct
     7801 gggccagctg ctcgagctcg gggcgcgcca gcatgacgcg cagctcgggt tgcccgatca
     7861 ggacgatctg cagcagcttg cgctcgctcg tctcgaggtt cgtcagaagg cgcagctgct
     7921 ccagcacgtc ggacgacagg ttctgcgcct cgtcgatgat cagcacgttg ttctggccaa
     7981 cggcgtgcgt gcgcagcagg tattcgttca ggccgtcgac gtagtccttg acgcccggcg
     8041 gcgtgccggc ggccgtcagc ggcgggaact tgatgccgaa ctcgtggcag acggtgacca
     8101 gcagctcgag cgccgacagc ttcgggttga agatgtaggc gacgttgcag cgcgccggga
     8161 tctgctccag gaagcagcgg cacacggtcg tcttgccggc accgatctcg cccgtcagca
     8221 gcacgaagcc gccgccgccc tggacgccgt agagcaggtg cgcgagcgcc tcccgatgac
     8281 gctcgctcat gaagaggtag cgtggatcgg gcgcgatcga gaacggcttc tgcttgaggc
     8341 cgaagaaggg cgcgtacatg gccatagccc gcaagcatac ttcggcgcgg tgaaggcggc
     8401 gcggggatgg ctctcgttgc agctgcacgc gctcgcggcg ccgaggcgtg cccgcgcggc
     8461 gggcgacggc cgcagggccg ggtggcccaa gaagcaccat ggccggggtg gatcctgcga
     8521 ctgcgcttcg ctccgcgcag gatgacggct tggcgcttcg cttcgcgcgg gaagtcggct
     8581 cggcgctcgc ttcgcgcggc gtgccggctt ggcatttcgc tggcgaggtg tccggccccg
     8641 acgcgctcaa gacgtgtcat cccgcacgca gtcgcaggat ccctcggcgt cggcctcggc
     8701 tctcgacggc ccgtgcagcc cgagccgcgc caggaactct cggcaaggcc gatctggact
     8761 atcgtcgcgg acatgcgcat ccaactggcc tccgacctgc acctggaact gctcgcctcc
     8821 acgtggccgc aggagcgcct cgtcgcgccg gcgcccggcg ccgacgtcct cgtgctggcc
     8881 ggcgacatcg accgcggcct gcgcaccatc gagcgcttcg ccgactggcc ggtgccggtg
     8941 ctctacgtgg ccggcaacca cgagttctac gaccacgaat gggagcagtt gcgcgacgac
     9001 ctgcgagatc gtgcggccgg cgtcgtcgac gcgcgcggcg ccggcctcga cgtcctgggt
     9061 ggacttctgg atcagcgcgc gga
//
LOCUS       DATCRO010000089         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_844873, whole genome shotgun sequence.
ACCESSION   DATCRO010000089 DATCRO010000000
VERSION     DATCRO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844873"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VIN75_02395"
     CDS             complement(<1..1168)
                     /locus_tag="VIN75_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8973038.1"
                     /translation="MDFAYSPRTLELQQRLQAFFAEHILPAEPRYFEEIEANTAAGRR
                     WTPLQLIEELKLKARSEGLWNLFLPDSERGAGLSNQDYAPLAEIMGRTPWASEVFNCS
                     APDTGNMEVLVRYGTPEHQRRWLEPLLDGRIRSAFAMTEPTVASSDATNIESRIVRDG
                     DDYVLNGRKWWTSGAGDPRCAVHIFMGKTDPEAPPHSQQSMILVPADAPGLTVLRPLT
                     VFGYDDAPHGHMEVAFDDVRVPASNMLLGEGRGFEIAQGRLGPGRIHHCMRLIGLAER
                     ALELMCERASKRVAFGKPIAAQTVTQERIAEARCQIDMARLLTLKAAWMMDVAGNKTA
                     KAEIAMIKVVAPNMACRVIDWAMQAFGAMGMSEDAPLAYFYTVARTLRFADGPDE"
     gene            complement(1170..2255)
                     /locus_tag="VIN75_02400"
     CDS             complement(1170..2255)
                     /locus_tag="VIN75_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8973039.1"
                     /translation="MNQTEQNTGTRPVAESHRFDNAALESFLAKNLAGFEGPLSVEQF
                     KGGQSNPTYKLVTPARSYVMRSKPGPVAKLLPSAHAVEREYRVMDALAKTDVPVARMN
                     LLCEDESVIGRAFYVMEYVEGRVLWDQGLPGMTPAERGAIYDEMNRVIAAMHNVDVGA
                     IGLADYGKPGNFFARQIGRWTKQYQASITEPIAEMDRLIEWLPVHMPASAMDESQVSI
                     VHGDFRMDNLIFHPTEPRVLAVLDWELSTLGHPLGDFSYHCMAWHIPPGTFRGIAGLD
                     HAALGIPSERDYVRRYCERTGRTDPDALLRDWNFYLAYNLFRLAAILQGIARRVVDGT
                     ASSAQARATGAGARPLAELAWQFAQRA"
     gene            complement(2313..3017)
                     /locus_tag="VIN75_02405"
     CDS             complement(2313..3017)
                     /locus_tag="VIN75_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8973040.1"
                     /translation="MNNPILTIEEQNNIDSGPWFSRLSDELRAAILSRAVVRRLPDGA
                     PLASRGSPAEDWCGVAKGAVRVSSVSLSGKQVSLTYVEPGTWFGDIALFDGLPRTHDA
                     DAHGATTLLSVRKADFKELLQQHVELYDALLRLNCRRLRIMFNLVEDLNTRPLSARLA
                     KNILMLAKAYGINQGDEVRIGLQLAQEDLAQLVGASRQRVNQELKELEREGALRIEPT
                     RIVVLSREKLVHAAER"
     gene            complement(3085..3759)
                     /locus_tag="VIN75_02410"
     CDS             complement(3085..3759)
                     /locus_tag="VIN75_02410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8973041.1"
                     /translation="MRHGSVDYFKADGSPVPPHTVPLNAAGRAQADAAGALFGAAGVR
                     FDRVVVSGLARTVETAQRVLAAAGQDAAIARDPRLEEIRSGRLDAIAPDQLRAAFTGV
                     FSASADVESLQFLGGETVGAMMDRCLPAFDEMLADTSWTCALLVLHGGINRALLGRAL
                     TGARLFLGRLEQAPACINVLDIGTGDIVVRGTNLAPTQWLHERERYTTMEKLLAQYLR
                     VGDIPV"
BASE COUNT          542 a         1307 c         1417 g          614 t
ORIGIN      
        1 cctcgtcggg gccgtcggcg aagcgcaggg tacgggccac ggtatagaag taggccagcg
       61 gcgcgtcctc ggacatgccc atcgcgccga acgcctgcat ggcccagtcg atcacgcggc
      121 aggccatgtt cggcgccacg accttgatca tcgcgatctc ggccttggcc gtcttgttgc
      181 ccgcaacgtc catcatccag gcggccttca gcgtgagcag gcgcgccatg tcgatctggc
      241 agcgcgcttc ggcgatgcgc tcctgcgtca ccgtctgcgc tgcgatcggc ttgccgaagg
      301 ccacgcgctt cgaggcgcgc tcgcacatca gctctagcgc gcgctcggcc aggccgatca
      361 ggcgcatgca gtggtggatg cggcccgggc ccagccggcc ctgggcgatc tcgaagccgc
      421 ggccttcgcc cagcagcatg ttcgaggcgg ggacgcgcac gtcgtcgaac gccacctcca
      481 tgtggccgtg cggcgcgtcg tcgtagccga acaccgtcag cgggcgcagc accgtcaggc
      541 ccggcgcgtc ggccggcacg aggatcatcg actgctgcga atgcggcggc gcctcggggt
      601 cggtcttgcc catgaagatg tggacggcgc agcgcggatc gccggcgccc gacgtccacc
      661 acttgcggcc gttgagcacg tagtcgtcgc cgtcgcggac gatgcgcgac tcgatgttgg
      721 tggcgtccga cgaggccacc gtgggctcgg tcatcgcgaa agcgctgcgg atgcggccgt
      781 ccagcagcgg ctccagccag cgccgctggt gctcgggcgt gccgtagcgc accagcactt
      841 ccatgttgcc ggtgtcgggc gccgagcagt tgaagacctc gctggcccac ggcgtgcggc
      901 ccatgatctc ggccagcggc gcgtagtcct ggttcgacag cccggcgccg cgttcgctat
      961 cgggcaggaa caggttccac agtccctccg agcgcgcctt gagcttgagc tcctcgatca
     1021 gctgcagcgg cgtccagcgc cggcccgcgg cggtgttggc ctcgatctcc tcgaagtagc
     1081 gcggctcggc gggcaggatg tgctcggcga agaaggcctg caggcgctgc tgcagttcga
     1141 gggtccgggg cgagtaggcg aagtccatgt caggcccttt gtgcgaattg ccaggcgagc
     1201 tcggcgagcg ggcgcgcgcc cgcgcccgtc gcgcgcgcct gcgcgctcga ggccgtgccg
     1261 tcgacgacgc gccgcgcgat gccctgcagg atggccgcga ggcggaacag gttgtaggcc
     1321 aggtagaagt tccagtcgcg cagcagcgcg tccgggtcgg tgcggccggt gcgctcgcag
     1381 tagcggcgca cgtagtcgcg ctcggacggg atgccgaggg ccgcgtggtc cagccccgcg
     1441 atgccgcgga aggtgccggg cgggatgtgc caggccatgc agtggtagct gaagtcgccc
     1501 agcgggtggc cgagcgtgga cagctcccag tccagcaccg ccagcacgcg cggctcggtc
     1561 gggtggaaga tcaggttgtc catgcggaag tcgccgtgca cgatggagac ctgcgactcg
     1621 tccatcgcgc tggcgggcat gtgcacgggc agccactcga tgaggcggtc catctccgcg
     1681 atcggttcgg tgatcgatgc ctggtactgc ttggtccagc ggccgatctg gcgcgcgaag
     1741 aagttgccgg gcttgccgta gtcggccagg ccgatcgcgc cgacatcgac gttgtgcatc
     1801 gccgcgatca cgcggttcat ctcgtcgtag atcgcgccgc gctcggcggg agtcatgccg
     1861 ggcaggccct ggtcccacag cacgcggccc tcgacgtatt ccatgacgta gaacgcgcgg
     1921 ccgatgacgg actcgtcctc gcacagaagg ttcatcctgg cgacgggcac gtccgtcttc
     1981 gcgagcgcgt ccatcacgcg gtactcgcgc tcgaccgcgt gggcggacgg cagcagcttc
     2041 gcgaccgggc ccggcttgga gcgcatcacg taggagcgcg ccggcgtgac gagcttgtag
     2101 gtcgggttgg actggccgcc cttgaactgc tcgacgctca gcgggccttc gaagcccgcg
     2161 aggttcttcg ccaggaagga ctcgagcgcc gcgttgtcga agcggtggct ctcggcgacc
     2221 gggcgggtgc cggtgttctg ttctgtctgg ttcatcggac ctgcctcaat gcttgtcgtg
     2281 gttcaacgca tcggcgacat gtccgatgca ctctatcgtt cggcggcgtg gacgagcttc
     2341 tcgcgcgaca gcacgacgat gcgtgtcggc tcgatgcgca gcgcgccttc gcgctccagc
     2401 tccttgagct cctggttgac gcgctggcgc gacgcgccca ccagctgcgc gaggtcctcc
     2461 tgggccagct gcaggccgat gcgcacctcg tcgccctggt tgatgccgta ggccttggcc
     2521 agcatcagga tgttcttggc cagccgcgcc gacagcgggc gcgtgttcag gtcctcgacg
     2581 aggttgaaca tgatgcgcag ccggcggcag ttcaggcgca gcagcgcgtc gtacagctcc
     2641 acgtgctgct gcagcagctc cttgaagtcg gccttgcgca ccgacagcag ggtggtggcg
     2701 ccatgcgcgt cggcgtcgtg ggtgcgcggc aggccgtcga acagcgcgat gtcgccgaac
     2761 caggtgcccg gctcgacgta ggtcagcgac acctgcttgc ccgacagcga caccgagctc
     2821 acgcgcaccg cgcccttggc cacgccgcac cagtcctccg cgggcgatcc gcgcgacgcg
     2881 agcggcgcgc catcggggag ccggcgcacc acggcgcgcg acaggatcgc ggcccgcagt
     2941 tcgtcggaaa gtctggagaa ccatgggccc gagtcgatgt tgttctgctc ttcgattgta
     3001 agaatcgggt tgttcattcc ttgtccctat cgcgacagcc ggcgggcgca attgatctgc
     3061 gccgattgtc gtctcaaaca ggactcagac aggaatgtcg cccacgcgca ggtactgcgc
     3121 cagcagcttc tccatcgtgg tgtatcgctc gcgctcgtgc agccactggg tgggcgcgag
     3181 attggtgccg cgcacgacga tgtcgccggt gccgatgtcc agcacgttga tgcaggccgg
     3241 cgcctgctcg aggcgcccga ggaacaggcg cgcgcccgtc agcgcgcggc ccagcagcgc
     3301 gcggttgatg ccgccgtgca gcaccagcag ggcgcacgtc cacgacgtgt cggccagcat
     3361 ctcgtcgaac gccggcaggc agcggtccat cattgcgccc accgtctcgc cgccgaggaa
     3421 ctgcagcgat tcgacatcgg cggaggcgct gaagacgccg gtgaacgcgg cgcgcagctg
     3481 gtcgggcgcg atggcgtcga ggcggccgga gcggatctcc tcgagtcgcg ggtcccgcgc
     3541 gatcgccgca tcctggccgg cggccgcgag cacgcgctgc gcggtctcga cggtgcgcgc
     3601 caggccgctg acgacgacgc ggtcgaagcg cacgccggcg gcgccgaaca gcgcgccggc
     3661 ggcgtcggcc tgggcgcgcc cggccgcgtt gagcggcacg gtgtgcggcg gcacggggct
     3721 gccgtcggcc ttgaagtagt cgacggagcc gtggcgcatc aggtagaggc gccggcgcag
     3781 gggcggtcgg acgtcggcga ccgacgggag cgggcgcggg gcggggcggt cggcggtcaa
     3841 gggcgggtcg ctgttgtatt tccgttgaag gtcgccggcc
//
LOCUS       DATCRO010000090         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_338553, whole genome shotgun sequence.
ACCESSION   DATCRO010000090 DATCRO010000000
VERSION     DATCRO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338553"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VIN75_02415"
     CDS             <1..491
                     /locus_tag="VIN75_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8973042.1"
                     /translation="VMALAILGNFCLGLYMTDLGMSMLRLKLYNYHKWTGATILALAA
                     LRLLWRLTHRPPAEPPAPRWQHTAASVTHWALYALFFAVPLVGWAYSSAAGFPIVVFG
                     VLPLPDFVPKDHALAEAIKPWHGDLAFVLFGLVCLHLAAVAKHMVIDRDGLIRRMLPG
                     RA"
     gene            520..1086
                     /locus_tag="VIN75_02420"
     CDS             520..1086
                     /locus_tag="VIN75_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8973043.1"
                     /translation="MTKFRTAFVLAASLALSGVAFAQAKVVPAQSQITFAIKEMGVPV
                     EGRFGQWTADIVFDPKKPEAGKVAFTIATASATLGNPETDAEVPKATWFNVAKFPNAT
                     FASTSIKAKGGGKFDVAGKLTVKGTSKDVVVPIALAQAGGNTTATGAFAIKRNEFHIG
                     EGEWTDTSQLADEVAVKFKILLTGVAPL"
     gene            1193..1765
                     /locus_tag="VIN75_02425"
     CDS             1193..1765
                     /locus_tag="VIN75_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8973044.1"
                     /translation="MFKSTLLALVAAIGVTAAHAESATYAIEPSHSFATFEIGHFGTS
                     TNRGRFNKTSGTVTIDKAAKTGSLDVTVDTTSLDTGTAAFTKHISSEEILNVAAFPTA
                     KLVATKFVFNGDKVAEIDGSLTLHGKTNPVVLKAQNFNCYQNPMLKREVCGGDFDATI
                     VRSQYDVSYGLNYGFPDNVHLVIQVEAVKQ"
     gene            1746..2360
                     /locus_tag="VIN75_02430"
     CDS             1746..2360
                     /locus_tag="VIN75_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8973045.1"
                     /translation="MKPSSSNLLRASRAALAALCASAGGLPALAQSVAGPVTYQLDPN
                     HTFVTFEVMHFGTSTLRGRIGPVNGEVTVDRAGKSGELRLRIPVTTLSTGSRALDARL
                     REPDLLATTEYPEAYFVATRFTFDAAGGVKEVRGEFTLRGVGEPLSLVARSFACRQDA
                     MLKRQVCGGDFDGQLERSRFGATLGLPFVGDDVHLVVQVEAIAP"
     gene            2435..3559
                     /locus_tag="VIN75_02435"
     CDS             2435..3559
                     /locus_tag="VIN75_02435"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8973046.1"
                     /translation="MTLPDDWRHDREIGVQPGTTLDTTRVDDVRIGAVRPLISPALLL
                     DDMPAPDSAVSLVEKARVELAKVLAGTDDRLVVVVGPCSIHDHDQALEYARHLKVVAD
                     ALKDDLLVVMRVYFEKPRTTVGWKGYINDPRLDGSFRINEGLRKARQLLLDVAEIGLP
                     AGTEFLDLLSPQYIADLVSWGAIGARTTESQSHRQLASGLSCPVGFKNGTDGGIKVAA
                     DAILAANASHAFMGMTKMGQAAIFETRGNADCHVILRGGKAPNYKAPDVAAACAALAE
                     RGLREQVMIDFSHANSEKKHQRQIDVGADVAGQIAGGDARITGVMIESHLADGRQDLK
                     PGVTPAWGVSITDACIGWADTEPLLGRLAQAVRERRAARR"
     gene            3556..3984
                     /locus_tag="VIN75_02440"
     CDS             3556..3984
                     /locus_tag="VIN75_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8973047.1"
                     /translation="MNGVATDDVQYVGFWARLVASLVDLVGIMFVVVPVTVFLIGDGW
                     AYAQGSAAFFINWVVPGAAYVAFWVFKGATPGKMLFKAQVVDAQTRAAPDFWQALTRY
                     VGFFVSVTPLLLGFIWVAFDARKQAWHDKMARTVVIYRRR"
BASE COUNT          617 a         1445 c         1324 g          602 t
ORIGIN      
        1 gggtcatggc gctggcgatc ctcgggaact tctgtctggg gctgtacatg accgacctgg
       61 gcatgtcgat gctgcggctc aagctctaca actatcacaa gtggacgggc gccacgatcc
      121 tcgcgctggc cgccctgcgc ctgctgtggc gcctgacgca ccgcccgccg gccgaaccgc
      181 cggcgcccag gtggcagcac acggccgcct cggtgacgca ctgggcgctg tacgcgctgt
      241 tcttcgccgt gccgctggtc ggctgggcct acagttcggc cgcgggcttc ccgatcgtgg
      301 tgttcggcgt gctgccgctg cccgacttcg tgcccaagga ccacgcgctg gccgaggcca
      361 tcaagccctg gcacggcgac ctcgccttcg tgctgttcgg cctcgtctgc ctgcacctgg
      421 ccgcggtcgc caagcacatg gtgatcgacc gcgacgggct gatccgccgc atgctccccg
      481 gccgcgcctg aacccgattc ctctcgaaag acccaccaca tgaccaagtt ccgcaccgcc
      541 ttcgtcctcg ccgcctcgct cgcgctgtcc ggcgtcgcct tcgcccaggc caaggtcgtc
      601 cccgcgcaga gccagatcac gttcgcgatc aaggagatgg gcgtgcccgt cgagggcagg
      661 ttcggccagt ggacggccga catcgtcttc gacccgaaga agcccgaggc cggcaaggtc
      721 gccttcacga tcgccacggc cagcgccacg ctgggcaatc cggagacgga cgccgaggtg
      781 cccaaggcga cctggttcaa cgtcgccaag ttccccaatg ccacgttcgc ctcgacgtcc
      841 atcaaggcca agggcggcgg caagttcgac gtcgccggca agctgacggt caagggcacc
      901 agcaaggacg tggtcgtgcc catcgcgctg gcccaggccg gcggcaacac caccgccacc
      961 ggtgcgttcg ccatcaagcg caacgagttc cacatcggcg aaggcgagtg gaccgacacc
     1021 tcgcagctgg ccgacgaggt ggccgtcaag ttcaagatcc tgctgaccgg cgtcgcgccg
     1081 ctctgaccgt tcgtcctgat ttcctccagc cccgtcacgg ggcggcagcc ggcggtgcgg
     1141 gtcagcgccg ggacgctttc ttccaacgaa tcctgacccc aggagccctc ccatgttcaa
     1201 gagcaccctg ctggccctcg tggctgccat cggcgtcacc gccgcccacg ccgaatcggc
     1261 cacctacgcc atcgaaccga gccactcgtt cgccacgttc gagatcggcc acttcggcac
     1321 gtcgaccaac cgcggccgct tcaacaagac cagcggcacc gtcacgatcg acaaggccgc
     1381 caagaccggc tcgctggacg tcaccgtcga caccacgtcg ctggacaccg gcaccgccgc
     1441 gttcacgaag cacatctcca gcgaggagat cctcaacgtc gctgccttcc cgacggccaa
     1501 gctcgtggcc accaagttcg tcttcaacgg cgacaaggtc gctgagatcg acggcagcct
     1561 gacgctgcac ggcaagacca acccggtcgt gctgaaggcg cagaacttca actgctacca
     1621 gaacccgatg ctcaagcgcg aagtctgcgg cggcgatttc gacgccacca tcgtgcgcag
     1681 ccagtacgac gtgtcgtacg gcctgaacta cggcttcccc gacaacgtgc acctcgtgat
     1741 ccaagttgaa gccgtcaagc agtaacctgc tgcgagcttc cagggcagcg ctcgccgcgc
     1801 tgtgcgccag cgccggcggc ctgcccgcgc tggcgcagtc cgtggcgggc ccggtcacgt
     1861 accagctcga tccgaaccac accttcgtca cgttcgaggt gatgcacttc ggcacgtcga
     1921 cgctgcgcgg gcgcatcggg cccgtgaacg gcgaggtgac ggtcgatcgc gcgggcaagt
     1981 cgggcgaact gcgcctgcgc atcccggtga cgacactgtc gacgggctcg cgcgcgctcg
     2041 acgcgcgcct gcgcgagccc gacctgctgg ccaccacgga gtaccccgag gcgtatttcg
     2101 tcgcgacgcg cttcaccttc gacgccgccg gcggcgtcaa ggaagtgcgc ggcgagttca
     2161 cgctgcgcgg cgtcggcgag ccgctgtcgc tggtggcgcg ctcgttcgcc tgccgccagg
     2221 acgcgatgct caagcgccag gtctgcggcg gcgacttcga cggccagctc gagcgcagcc
     2281 gcttcggggc gacgttgggg ctgcccttcg tcggcgacga cgtgcacctg gtggtccagg
     2341 tcgaggcgat tgccccctga tgcccggccg caacgcggct gcccgataat cgggcacgcg
     2401 ccgcctcggc gcgaggattc ctgaaagctt ggcgatgacc ctccctgacg attggcgcca
     2461 cgaccgcgag atcggcgtgc aacccggcac gacgctggac accacgcgcg tcgacgacgt
     2521 gcgcatcggc gccgtccgcc cgctcatctc cccggcgctg ctgctggacg acatgcccgc
     2581 tcccgacagc gcggtgtcgc tggtcgagaa ggcccgggtc gagctggcca aggtgctggc
     2641 cggcacggac gaccggctgg tcgtcgtcgt cggcccgtgc tcgatccacg accacgacca
     2701 ggcgctggag tacgcgcgcc acctgaaggt cgtggccgac gcgctcaagg acgacctgct
     2761 cgtcgtgatg cgcgtgtact tcgagaagcc gcgcaccacc gtcggctgga agggctacat
     2821 caacgacccg cgtctggacg gcagcttccg catcaacgag ggcctgcgca aggcccgcca
     2881 gctgctgctg gacgtggccg agatcggcct gcccgcgggc accgagttcc tcgacctgct
     2941 gagcccgcag tacatcgccg acctggtgtc ctggggcgcg atcggggcgc gcaccaccga
     3001 gagccagagc catcgccagc tcgcctcggg cctgtcgtgc ccggtgggct tcaagaacgg
     3061 caccgacggc ggcatcaagg tggcggccga cgccatcctc gcggccaacg cgtcgcacgc
     3121 cttcatgggc atgaccaaga tgggccaggc cgcgatcttc gagacgcgcg gcaatgccga
     3181 ctgccacgtg atcctgcgcg gcggcaaggc gcccaactac aaggcgcccg acgtcgcggc
     3241 ggcctgcgcg gcgctggccg agcgcggctt gcgcgaacag gtgatgatcg atttctcgca
     3301 cgccaactcc gagaagaagc accagcgcca gatcgacgtc ggcgcggacg tcgccggcca
     3361 gatcgcgggc ggcgacgcgc gcatcaccgg cgtgatgatc gagagccacc tggccgatgg
     3421 ccgccaggac ctgaagccgg gcgtgacgcc ggcctggggc gtgtccatca ccgacgcgtg
     3481 catcggctgg gccgacaccg agccgctgct gggccgcctc gcgcaggccg tgcgcgagcg
     3541 ccgcgccgcg cgccgatgaa cggcgtcgcg acggacgacg tccagtacgt cgggttctgg
     3601 gcgcgcctgg tcgcctcgct ggtcgacctg gtgggcatca tgttcgtcgt ggtgcccgtc
     3661 acggtgttcc tgatcggcga cggctgggcg tacgcgcagg gcagcgcggc cttcttcatc
     3721 aactgggtcg tcccgggcgc ggcctacgtc gcgttctggg tgttcaaggg cgcgacaccg
     3781 ggcaagatgc tgttcaaggc ccaggtggtg gacgcgcaga cgcgcgccgc gccggacttc
     3841 tggcaggcgc tgacgcgcta cgtcggcttc ttcgtgtcgg tgacgccgct gctgctgggc
     3901 ttcatctggg tggcctttga cgcgcgcaag caggcctggc atgacaagat ggcccgcacc
     3961 gtggtgatct accgccggcg ctgaagcg
//
LOCUS       DATCRO010000091         8230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_991093, whole genome shotgun sequence.
ACCESSION   DATCRO010000091 DATCRO010000000
VERSION     DATCRO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991093"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..3436
                     /locus_tag="VIN75_02445"
     CDS             437..3436
                     /locus_tag="VIN75_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973048.1"
                     /translation="MNQHHSTRARVFRRSLLAMACAAAAAPAFAQTAPAAAPAASAAS
                     APAAVAPAAPESAQQVVVSGSRIKHDTFSSPAPLQIIKSEDSALAGVTTVAGILQSTA
                     VTGGQGQINNAFGGYVTDGGPGANTLGLRGLSPTRTLILLNGRRLSPSGTRGSVGAAD
                     LNVLPASIVDRVEILKDGASSIYGSDAIAGVVNIITKKDLTEINVDGGTSQTAHGGGN
                     QYNFSLSGGLVTDHAHFLASYSFEEQQELKMGQRSWASCGNDYTRDFDADGNPLPWGS
                     GDFVDPVTGKPKCFPITATGSQGVTINTIGTSRTAGQPGPGTPAASGYTRWRPNSGVT
                     SGLAGWEGVSLQSRDTFDPRMLNQSLLSPAKNHNLFFQGGVDLPALGDTTEFYWEAMY
                     NRRESQQTGYRQLALDYAVGSPLIPSNLQFSDALGPTSISNGNDVGVRAFIGTGNDHS
                     SQSVNFTRAVAGLRGNFSKINWDYDVSLTHSQSRGTYAFDGFRTDRLAQSLDVVANAN
                     GGYDCVDPSNGCVAAPALTSAVVGGHLPASWLNYVFDPKIQGSTKYKEDVLTASTTGD
                     IYTLPYGKLKGAFGLEYRHNSIDDVPSEDMQTGNIYNFTSSTETRGSDSAKDVFGEVE
                     VPVLKGLPFAHELTLNGSLRRSDYKSYGAGTTHKFGGLYSPFKWVSVRGTMGTSYRAP
                     ALFEQYVGATTGFLSASVDPCNDWDQKTGNVATNCAAEGLPAGFQQTNGVTDITSGGR
                     SSGLKAETSKNNSWGIILQPDMPTGWGDLSFAFDHYNINVNNGISQIGYSNILSLCYN
                     DPNFRNAGSYCRLVTRDPTSHALTINDSYINVASNLVRGNDYTLSYANNVGIGRLTVD
                     MQATQYLMQANKLFSDDPWSDINGIIGSPKWSGEFDVSYKVKNWKLHWATEVVGKTNE
                     YAYDGEDPASSNLKLTTPTYLLHSMSVTYEEPVSKWKATIGVRNLFDKNPPAISSGYD
                     NRIGTAPLYSGYDLFGRAFFASLSKTF"
     gene            3511..5583
                     /locus_tag="VIN75_02450"
     CDS             3511..5583
                     /locus_tag="VIN75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8973049.1"
                     /translation="MRTILLRSRMSVLAAIATASLLAGAQALATDAGPIKVPSLETLA
                     QFPDMTGFQLSPDGKHMLAIKSEGDNRSILVWETARLSAKPTVIGSKSMQISGASFLK
                     NDMLAVHMSQPYDLRAGRVVKTFITKLLFTDLEGKEWTEPMAAVGAGRSSDEQIEDAL
                     SNPTVMRRMADDPDNVILGSDGHGKGRALFRYNVHTHSATRLMRLSDVDNDVWVNAAG
                     QPWAKSRGNSDDKGAYIAIDFRGEDGEWQEHFRSYVKDRDEINVVAVGTKPGTAVILS
                     NVGHEFAGLYEYDIASRKVTSTLFEHKYFDASGVRSLRAEGNPGKDGYDGFTYEGAMG
                     DEDVWVDPKIDALLRGVAQALGIKETNQELVGVTDGKKSTVRSFDGVSVRLKQYVAGD
                     VPTYLIHVTGLAYPSEDYLMRGQSLQLLAKEYPAIDRRALGTSGLVYYPARDGLNIPA
                     YLTTPNPALCGPGPYAAVIHPHGGPWARDTMEYDWSAWVPMLVSQCRVVLQPQYRGST
                     GWGRTLWFKGDREWGQKMQDDKDDGVKWLTAQKLIDPKRVAMFGFSYGGYAAFAASVR
                     PNGLYKCAIAGAGVSDIERIWARFYDNPFYQEHQESTVRGLSPLTLADKIQIPIMVYH
                     GDRDQTVPLIQSQMFVDKAKAAGKPVEFHVLTDYAHGPAWKRETNTEQLKLISDYLGK
                     GGGCAGGL"
     gene            complement(5666..6097)
                     /locus_tag="VIN75_02455"
     CDS             complement(5666..6097)
                     /locus_tag="VIN75_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEY8973050.1"
                     /translation="MLVRLMYASRAAANLDNEEVAAILRKSRATNAREGLTGALCLCN
                     SGRLFIQVLEGGREAVSRRYNRIVADPRHTDVTLLQFEEIGERRFAGWSMGQVNMARL
                     NPGLILKYSELGNLDPWSVPGSATAALLDEMIATAAIMGQP"
     gene            complement(6107..6883)
                     /gene="folE"
                     /locus_tag="VIN75_02460"
     CDS             complement(6107..6883)
                     /gene="folE"
                     /locus_tag="VIN75_02460"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY8973051.1"
                     /translation="MPTPVPPSDSTPNAGDATSRSLTLIRSEADLAALPASERIRYRL
                     VGADCRFHANDNISDYVRPGETEELKAEVAEKMQAVLRALVIDTDSDHNTQETAQRVA
                     KMFVDEVFRGRYQPMPSVTEFPNVSRLNELMIVGPISVRSACSHHMCPILGRIWIGVL
                     PNEHSNLIGLSKYSRIVDWIMTRPQIQEEAVTMLANVLQERVKPDGLAIVMEADHFCM
                     QWRGVKDLDSAMTNSVMRGAFLKDPHLRREFLSLLSKKTA"
     gene            7086..>8230
                     /gene="rsmB"
                     /locus_tag="VIN75_02465"
     CDS             7086..>8230
                     /gene="rsmB"
                     /locus_tag="VIN75_02465"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY8973052.1"
                     /translation="MNPDDSPRAAPAAQAPALARLLEQTADAVQAVRAGRSLTDVLAA
                     VPAPLRPGTQALSFLVLRRLGAAEALRAALAPRKPPPEIEGLLLGALALLWPDEAPPY
                     PDHTLVDQAVTAARRRAPRSASFLNAVLRRFVRERDALVDAALRQPVARWNHPQWWLD
                     RLKADWPGQWQGILAAAQARPPMCLRVNVRQATGVQYLERLEAAGLAGRLVGPQAIVL
                     DAPVPVERLPGFADGAVSVQDAAAQRAAELLVGAGLPPGARVLDACAAPGGKTAHLLE
                     LADLDVLALDADPQRLARVDATLARLRLPARTRAGDGRRPADWWDGRPFDAILLDAPC
                     SASGIVRRHPDIRWLRRPEDIPALGRVQAELLDALWPTLKPGGWLLYA"
BASE COUNT         1320 a         2980 c         2723 g         1207 t
ORIGIN      
        1 gagccgcgcc tgctgttcgt ccgccacggc ttcaacgacg cggtcgaccg ctacaacacc
       61 gccatccatc aatggccgac gcgcctcatc gcgtcgccgc tgggcttccg ttcagcgggc
      121 cgaatctgac ggacctgtca gcggaccttg actagacagg tatgcacctg tgtagtgcgg
      181 gggcattcag ccgcacctca gtgttgcagc cgcgtcacat tttttgtgcc tgaacggtcc
      241 gtcagcgggc cggtccgcgg gaccttcgaa cagcgctttc cccagggaag aaggctcgtt
      301 tcgtgggatt gcacacgaat tgagaaagga gctgcgcgct agaggattac ccgttgaggg
      361 tgagcacgag gattgcattc gggaaacctt ggttaacgtg cccagcactt ttcttactgc
      421 tccacccagg acttcaatga accaacacca ctcgacgcgc gcccgcgtgt tccgacgctc
      481 cctgctggcg atggcctgtg ccgccgccgc tgctcccgcc ttcgcgcaga ccgctcccgc
      541 cgcggccccg gctgcttccg ccgcttccgc gcccgccgcg gtcgctccgg ccgcgccgga
      601 gagcgcgcag caggtcgtcg tctccggctc gcgcatcaag cacgacacct tcagcagccc
      661 cgcgccgctg cagatcatca agagcgaaga ctcggcgctg gccggcgtca ccaccgtcgc
      721 gggcatcctg cagagcacgg ccgtcaccgg cggccagggc cagatcaaca acgccttcgg
      781 cggctacgtc accgacggcg gcccgggcgc caacacgctg ggcctgcgcg gcctgtcgcc
      841 cacccgcacc ctgatcctgc tgaacggccg ccgcctgtcg ccctcgggca cgcgcggctc
      901 ggtcggcgcg gccgacctga acgtgctgcc ggcctcgatc gtcgatcgcg tcgaaatcct
      961 caaggacggc gcctcgtcca tctacggctc cgacgccatc gccggcgtgg tcaacatcat
     1021 caccaagaag gacctgaccg agatcaacgt cgacggcggg acctcgcaga ccgcgcacgg
     1081 tggcggcaac cagtacaact tctcgctgtc gggcggcctg gtcaccgacc acgcgcactt
     1141 cctggcctcc tactcgttcg aggaacagca ggagctcaag atgggccagc gcagctgggc
     1201 cagctgcggc aacgactaca cgcgcgactt cgacgccgac ggcaacccgc tgccctgggg
     1261 cagcggcgac ttcgtcgacc ccgtcaccgg caagcccaag tgcttcccga tcacggccac
     1321 gggctcgcag ggcgtgacga tcaacaccat cggcacgagc aggaccgccg gccagcccgg
     1381 tccgggcacc ccggccgcct cgggctacac gcgctggcgc ccgaactcgg gcgtcacgtc
     1441 gggcctggcg ggctgggaag gcgtcagcct ccagagccgc gacaccttcg acccgcgcat
     1501 gctcaaccag tcgctgctgt cgccggccaa gaaccacaac ctgttcttcc agggcggcgt
     1561 ggacctgccg gcgctcggtg acaccaccga gttctactgg gaagcgatgt acaaccgccg
     1621 cgagtcgcag cagaccggct atcgccagct ggccctggac tacgcggtcg gcagcccgct
     1681 gatcccgtcc aacctgcagt tcagcgacgc cctgggcccc acgtcgatca gcaacggcaa
     1741 cgacgtcggc gtgcgcgcct tcatcggcac gggcaacgac cactcgagcc agtcggtgaa
     1801 cttcacgcgc gccgtcgcag gcctgcgcgg caacttcagc aagatcaact gggactacga
     1861 cgtctcgctg acgcacagcc agagccgcgg cacctacgcc ttcgacggct tccgcaccga
     1921 ccgcctggcg cagagcctgg acgtggtcgc gaacgccaac ggcggctacg actgcgtcga
     1981 cccgtccaac ggctgcgtgg ccgcgcccgc gctgacctcg gccgtcgtcg gcggccacct
     2041 gccggcgtcg tggctgaact acgtcttcga cccgaagatc cagggctcca cgaagtacaa
     2101 ggaagacgtc ctgacggcct cgacgaccgg cgacatctac acgctgccgt acggcaagct
     2161 caagggcgcg ttcggcctcg agtaccgcca caactccatc gacgacgtgc cgtcggagga
     2221 catgcagacg ggcaacatct acaacttcac gtcgtccacc gagacccgcg gcagcgactc
     2281 ggccaaggac gtcttcggcg aagtcgaggt gccggtcctc aagggcctgc cgttcgcgca
     2341 cgagctgacg ctgaacggct cgctgcgccg ctccgactac aagtcgtacg gcgcgggcac
     2401 cacccacaaa ttcggcggcc tgtactcgcc gttcaagtgg gtgtcggtgc gcggcacgat
     2461 gggcacctcc tatcgcgccc cggcgctgtt cgagcagtac gtcggcgcga cgacgggctt
     2521 cctgtcggcg tcggtcgatc cctgcaacga ctgggaccag aagaccggca acgtggccac
     2581 gaactgcgcg gccgaaggcc tgccggccgg cttccagcag accaacggcg tgaccgacat
     2641 cacgtcgggc ggccgcagct cgggcctgaa ggcggagacc tccaagaaca actcctgggg
     2701 catcatcctg cagccggaca tgcccacggg ctggggtgac ctgtcgttcg cgttcgatca
     2761 ctacaacatc aacgtcaaca acggcatcag ccagatcggc tacagcaaca tcctgtcgct
     2821 gtgctacaac gacccgaact tccgcaacgc gggcagctac tgccgcctcg tgacgcgcga
     2881 tccgacgtcg cacgcgctga cgatcaacga cagctacatc aacgtcgcca gcaacctggt
     2941 gcgcggcaac gactacacgc tctcgtacgc gaacaacgtg ggcatcggca ggctgaccgt
     3001 cgacatgcag gccacgcagt acctgatgca ggccaacaag ctgttctcgg acgatccgtg
     3061 gtcggacatc aacggcatca tcggttcgcc gaagtggtcg ggcgagttcg acgtgagcta
     3121 caaggtcaag aactggaagc tgcactgggc cacggaagtc gtcggcaaga cgaacgagta
     3181 cgcctacgac ggcgaggatc cggccagcag caacctcaag ctgaccaccc cgacgtacct
     3241 gctgcactcg atgtcggtga cgtacgagga gccggtgagc aagtggaagg cgacgatcgg
     3301 cgtgcgcaac ctgttcgaca agaacccgcc cgcgatctcg tccggctacg acaaccgcat
     3361 cggcacggct cccctgtaca gcggttatga cctgttcggc cgcgcgttct tcgcgagcct
     3421 gtccaagacc ttctgatcga ccgacggtcg atgctccaat gccctggcga ccccggtcgc
     3481 cggggctttt tccttcctgg gaagcatcca atgagaacaa tcctcctgcg ctcgcgcatg
     3541 agcgtcctgg ccgcgatcgc gacggcctcc ctgctggccg gcgcgcaggc cctggcgacc
     3601 gacgcggggc cgatcaaggt gccgtccctc gagacgctgg cgcaattccc cgacatgacg
     3661 ggcttccagc tctcgcccga cggcaagcac atgctggcca tcaagagcga gggcgacaac
     3721 cgctccatcc tggtgtggga gacggccagg ctgtcggcca agccgaccgt catcggctcg
     3781 aagtccatgc agatcagcgg cgcgtcgttc ctgaagaacg acatgctggc ggtgcacatg
     3841 tcgcagcctt acgacctgcg tgccggccgc gtggtcaaga ccttcatcac caagttgctg
     3901 ttcaccgatc tcgaaggcaa ggagtggact gagccgatgg ccgccgtcgg cgcgggccgc
     3961 tcgagcgacg agcagatcga ggacgccctg tccaacccca cggtgatgcg ccggatggcc
     4021 gacgaccccg acaacgtcat cctgggcagc gacggccacg gcaagggccg ggccctgttc
     4081 cgctacaacg tgcacacgca ttcggccacg cgcctcatgc gcctgagcga cgtcgacaac
     4141 gacgtgtggg tcaacgcggc cggccagccg tgggccaagt cgcgcgggaa ctccgacgac
     4201 aagggcgcct acatcgccat cgatttccgc ggcgaggacg gcgagtggca ggagcatttc
     4261 cgcagctacg tcaaggatcg cgacgagatc aacgtggtcg ccgtcggtac gaagcccggc
     4321 accgcggtga tcctgagcaa cgtcggccac gaattcgccg gcctgtacga atacgacatc
     4381 gccagccgca aggtgacctc cacgctgttc gagcacaagt acttcgacgc gagcggcgtg
     4441 cgctcgctgc gcgccgaggg caaccccggc aaggacggct acgacggctt cacgtacgag
     4501 ggcgccatgg gcgacgagga cgtctgggtc gacccgaaga tcgacgcgct gctgcgcggc
     4561 gtggcgcagg cgctgggcat caaggagacg aaccaggagc tggtcggcgt caccgacggc
     4621 aagaagtcca ccgtgcgctc gttcgacggt gtctcggtgc gcctgaagca gtatgtcgcg
     4681 ggcgacgtgc cgacatatct gatccacgtc accggcctcg cctatccgtc cgaggactac
     4741 ctgatgcgcg gccagagcct gcagttgctg gccaaggagt acccggccat cgacaggcgc
     4801 gcgctcggca cgtccgggct cgtctactac ccggcgcgcg acggcctgaa catcccggcc
     4861 tacctgacga cgccgaaccc ggcgctgtgc ggtccgggcc cgtacgcggc ggtcatccat
     4921 ccgcacggcg gcccctgggc gcgcgacacg atggagtacg actggtcggc ctgggtgccg
     4981 atgctggtct cgcagtgccg cgtcgtgctg cagccgcagt accgcggctc gaccggctgg
     5041 ggccggaccc tgtggttcaa gggcgaccgc gagtggggcc agaagatgca ggacgacaag
     5101 gacgacggcg tgaagtggct cacggcgcag aagctgatcg acccgaagcg cgtcgcgatg
     5161 ttcggcttct cgtacggcgg ctacgcggcc ttcgccgcct cggtgcggcc gaacggcctg
     5221 tacaagtgcg ccatcgccgg cgcgggcgtc tccgacatcg agcgcatctg ggctcggttc
     5281 tacgacaacc cgttctacca ggaacaccag gagagcaccg tgcgcggcct gagcccgctc
     5341 acgctggccg acaagatcca gattcccatc atggtctacc acggggatcg cgaccagacg
     5401 gtgccgctga tccagtccca gatgttcgtc gacaaggcca aggccgccgg caagcccgtg
     5461 gagttccacg tgctgaccga ctatgcgcac ggcccggcgt ggaagcgtga gaccaacacg
     5521 gaacagctca agctgatcag cgactacctc ggcaagggcg gcggctgcgc aggcggcctt
     5581 tgagcccggg ggcgggcgcg acctcaggcc ggtcgatccc gccctgacac ctgcatgccg
     5641 ccccgcccgc ccgggcggcg tcgtttcagg gctggcccat gatcgccgcg gtggcgatca
     5701 tctcgtccag cagcgccgcg gtggccgagc cgggcaccga ccatgggtcg aggttgccca
     5761 gctccgagta cttcaggatc aggcccgggt tcagccgcgc catgttgacc tggcccatgg
     5821 accagccggc gaagcggcgc tcgccgatct cctcgaactg cagcagcgtg acgtcggtgt
     5881 ggcgcgggtc ggccacgatg cggttgtagc gccggctgac cgcctcgcgc ccgccctcga
     5941 gcacctggat gaacaggcgc ccgctgttgc acaggcacag cgcgccggtc aggccctcgc
     6001 gcgcgttggt cgcgcgcgac ttgcgcagga tggcggccac ttcctcgttg tcgaggttgg
     6061 cggccgcgcg gcttgcatac atcaggcgca ccagcatggc ggctcctcag gcggtcttct
     6121 tggacagcag cgacaggaat tcgcggcgca ggtgcggatc cttcaggaac gcgccgcgca
     6181 tgacgctgtt ggtcatcgcg gaatccagat ccttcacgcc gcgccactgc atgcagaagt
     6241 ggtcggcctc catgacgatg gccaggccgt cgggcttgac gcgctcctgc agcacgttgg
     6301 ccagcatcgt gaccgcctct tcctggatct gcggccgcgt catgatccag tcgacgatgc
     6361 gcgagtactt ggacaggccg atcaggttcg agtgctcgtt gggcagcacg ccgatccaga
     6421 tgcggcccag gatcgggcac atgtggtgcg agcacgcgct gcgcaccgag atcgggccga
     6481 cgatcatcag ctcgttcagc cgcgacacgt tcgggaactc ggtgaccgag ggcatcggct
     6541 ggtagcggcc gcggaacacc tcgtcgacga acatcttggc cacgcgctgc gcggtctcct
     6601 gcgtgttgtg gtcggagtcg gtgtcgatca ccagcgcccg cagcacggcc tgcatcttct
     6661 cggccacctc ggccttgagc tcctcggtct cgcccgggcg gacgtagtcg gagatgttgt
     6721 cgttggcgtg gaagcggcag tcggcgccca cgagccggta gcggatgcgc tcggacgcgg
     6781 gcagcgcggc caggtcggcc tcgctgcgga tcagcgtcag cgagcgggac gtggcgtcgc
     6841 cggcgttggg cgtggagtcg gacggcggga cgggagtggg catgtaacgg cctggcctgg
     6901 gaaatctttc cggggcgggt attttgcgcc gccgccacgt ttcgcgcctc cggcgcgcct
     6961 tccggccctc ctttgcccgg gacatccgcg cccgcgggcc ggccgggtcg cgcgcgcccc
     7021 acacgttggc cgtcccgaca aggcgccgac gcccggcggc aacccccgct cgttagactg
     7081 gccgggtgaa tcccgacgac tccccccgcg ccgcgcccgc cgcccaggcg ccggcgctcg
     7141 cgcgcctgct cgaacagacc gccgacgccg tgcaggccgt gcgcgccggc cgctcgctca
     7201 ccgacgtgct cgccgccgtc ccggcgccgc tgcggccggg cacccaggcc ctgtcattcc
     7261 tcgtgctgcg ccggctcggt gccgccgagg ccctgcgcgc cgcgctcgcg ccgcgcaagc
     7321 cgccgccgga gatcgagggg ctgctgctcg gcgcgctggc cctgctgtgg cccgacgagg
     7381 cgccgcccta cccggatcac acgctggtcg accaggccgt caccgccgcg cgccgccgcg
     7441 cgccgcgctc ggcgtccttc ctcaacgccg tgctgcggcg cttcgtgcgc gagcgcgacg
     7501 cgctggtcga cgcggcgctg agacagcccg tcgcacgctg gaaccatccg cagtggtggc
     7561 tcgaccgcct gaaggccgac tggcccgggc agtggcaggg gatcctcgcg gccgcccagg
     7621 cgcgcccgcc gatgtgcctg cgcgtgaacg tgcgccaggc gacgggcgtg cagtacctcg
     7681 agcgactgga ggcggccggc ctggccggcc ggctggtcgg gccgcaggcc atcgtgctgg
     7741 acgcgccggt gcccgtcgag cgcctgccgg gtttcgccga cggcgccgtc tccgtgcagg
     7801 acgccgccgc ccagcgcgcg gccgaactgc tggtgggcgc cggcctgcca ccgggtgccc
     7861 gggtgctgga cgcctgcgcc gcccccggcg gcaagacggc tcacctgctg gagctggccg
     7921 acctggacgt gctggccctc gacgccgacc cgcagcgcct cgcgcgcgtg gacgcgacgc
     7981 tggcgcggct gcgcctgccg gcgcgcaccc gcgcgggcga cggccgccgc cccgccgact
     8041 ggtgggacgg ccggccgttc gacgccatcc tgctcgacgc cccgtgcagc gcctcgggca
     8101 tcgtgcgccg ccaccccgac atccgctggc tgcgccgccc cgaggacatc ccggcgctcg
     8161 gccgcgtcca ggccgagctg ctggacgccc tgtggcccac gctcaagccg ggcggctggc
     8221 tgctctacgc
//
LOCUS       DATCRO010000092         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_406037, whole genome shotgun sequence.
ACCESSION   DATCRO010000092 DATCRO010000000
VERSION     DATCRO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406037"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VIN75_02470"
     CDS             <1..277
                     /locus_tag="VIN75_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HEY8973053.1"
                     /translation="AAPLLLLAIAAADHGWPSLARRLSRHPKLGPVVAAWRERGAMPV
                     GLKLLAIGGLAFSAGAVWLLPMPMWWKALLVVSLVLFGVWMWRRPSR"
     gene            complement(291..>2228)
                     /locus_tag="VIN75_02475"
     CDS             complement(291..>2228)
                     /locus_tag="VIN75_02475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY8973054.1"
                     /translation="SIGSAITSIASSATPLELHLRWLVRAFGIAAAGISVFLLIWYGL
                     HSGRWVQGLLAGLALAMAMLPEEFPMVFAVFLALGAWRLARVRVLARRPAVIEALGAA
                     TFLCVDKTGTLTENRMRLRWLASVDGRLSAPDDPRLSDGHRELLEAARLATRPHTADP
                     MEQAVFAAAVHQPAPGLHEQGQPVLERDYALQSDLLAMTLVWRDATGARRIAAKGAVE
                     AICELCHLTEADAQALMRRAGDLAEAGLRVLAVARGRLASDTLPASQHDMAFEPLGLL
                     AFEDPLRAGVPQAVAQAHGAGIAVAMITGDHAATALSIAKQAGIHTGEGALTGKQMEA
                     LDEAGLRAAVRGVRVFARVQPEQKLRLVRALAANGETVAMTGDGVNDAPALKAAHIGI
                     AMGARGTDVAREAAGLVLLDDDFGHIVAGVRMGRRIFDNLRRVMTYITAIHVPIAGLA
                     LLPVLLGLPPLLLPVHVVLTEMVVDPACSLAFEGAPDDADIMARPPRASDADLVGWPM
                     LWRGLLQGGALLAATLLAFSLEMSRSGSEETSRTCAMAGLTLGNLLLVAVDATAGKGL
                     RALLTRDFRAFWVVAFIAGSTIAAAIAWPPLRALMHFGVPSGLDLAATLAGCGASVFL
                     VRSLVGMTATRHGAVLAPRTA"
BASE COUNT          331 a          800 c          807 g          290 t
ORIGIN      
        1 ggcggcgccg ctgctgctgc tggcgatcgc cgcggccgac catggatggc catcgctggc
       61 tcgtcgcctg tctcgccacc cgaagctcgg gccggtggtg gccgcctggc gcgagcgcgg
      121 agcgatgccc gtggggctga agctgctggc catcggcggc ctcgccttca gcgccggcgc
      181 cgtgtggctg ttgccgatgc ccatgtggtg gaaggcgctg ctggtcgtct ctctcgtgct
      241 gttcggcgtc tggatgtggc gccgtccgag tcgctgactt gcgccgctgt tcatgcggta
      301 cgcggcgcca gcaccgcccc gtgccgggtc gccgtcatgc ccaccagcga gcgcaccagg
      361 aacaccgatg cgccacagcc cgccagcgtc gcggccaggt cgagccccga tggcacgccg
      421 aaatgcatca aagcgcgcag cggtggccac gcgatcgccg cggcgatggt cgagccggcg
      481 atgaaggcca cgacccagaa cgccctgaag tcgcgtgtca gcaaggcacg caggcccttg
      541 ccggctgtcg catcgaccgc gacgagcagc aggttgccca gcgtcagtcc ggccatggcg
      601 caggtgcgtg acgtctcctc ggatccggag cgcgacatct ccagggagaa cgcgagcagc
      661 gtcgccgcca ggagcgcgcc gccttgcagc aggccgcgcc agagcatcgg ccatccgacg
      721 aggtccgcat cgctggcgcg cggcggccgc gccatgatgt ccgcatcgtc cggcgcgcct
      781 tcgaacgcga gagagcacgc cggatcgacc accatttccg tgagcaccac gtggacgggc
      841 aagaggagcg gcgggagtcc gagcaggacg ggcaacagcg ccagcccggc gatcgggacg
      901 tggatggcgg tgatgtaggt catcacgcga cgcaggttgt cgaagatgcg ccggcccatg
      961 cgcacgccgg cgacgatgtg gccgaagtcg tcgtccagca gcacgagtcc cgccgcctcg
     1021 cgcgcgacgt cggtgccgcg cgcgcccatg gcgatgccga tgtgggccgc cttcaacgcg
     1081 ggcgcgtcgt tgacgccgtc gccggtcatg gccacggtct cgccgttggc ggccagggcg
     1141 cggacgagcc gcagcttctg ctccggttgc acgcgcgcga acacccgcac gccacgaacc
     1201 gccgcccgca ggccggcctc gtcgagagcc tccatctgct tgccggtcaa cgcgccttcg
     1261 ccggtgtgga tgccggcctg cttggcgatg gacagcgccg tggcggcgtg gtcgccggtg
     1321 atcatcgcca cggcgatgcc cgcgccgtgc gcctgcgcca ccgcctgcgg aaccccggcg
     1381 cgcagcggat cctcgaaggc gagcagcccc agtggctcga aggccatgtc gtgctgggac
     1441 gccggaagcg tgtccgacgc cagcctgccc cgcgcgaccg ccaggacgcg cagtcctgct
     1501 tcggccagat cgccagcacg ccgcatgagt gcctgcgcat cggcttcggt caggtggcaa
     1561 agctcgcaga tggcctccac ggcgcccttg gcggcgatcc ggcgcgcgcc ggtggcgtcg
     1621 cgccagacga gggtcatcgc gaggaggtcc gactgcaacg cgtaatcgcg ctcgagaaca
     1681 ggctgcccct gctcatgaag accaggggcc ggctggtgga cggccgccgc gaagacggcc
     1741 tgttccatcg gatcggcggt gtgcgggcgc gtcgccaggc gggccgcctc gagcagttcg
     1801 cgatggccgt ccgacagccg tggatcgtcc ggcgccgaca agcggccgtc cacgctcgcg
     1861 agccagcgca ggcgcatgcg gttctcggtc agcgtccccg tcttgtcgac gcagaggaag
     1921 gtcgccgcgc ccagcgcctc gatgacggcc ggccggcggg ccagcacccg gactcgagcc
     1981 agccgccagg cgcccagggc gaggaacacc gcgaagacca tcgggaactc ctcgggcagc
     2041 atggccatcg ccagggccag gccggcgagc aggccctgca cccagcggcc ggagtgcagt
     2101 ccataccaga tcaggaggaa gacgctgatg ccggccgcgg caatgccgaa cgcccgcacc
     2161 agccagcgca gatgcagctc gagcggggtc gccgacgaag cgatcgaggt gatggcgctg
     2221 ccgatgct
//
LOCUS       DATCRO010000093         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_406039, whole genome shotgun sequence.
ACCESSION   DATCRO010000093 DATCRO010000000
VERSION     DATCRO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406039"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VIN75_02480"
     CDS             <1..621
                     /locus_tag="VIN75_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8973055.1"
                     /translation="VLRRADAAMAHAPAAFMVTAPPYLRPSQDALRAFFTTIADALPA
                     PLVVYDIPARTGVRIELDTLLALAAHPNIVGLKDCGGRVEQTEALIADGRLQVLCGND
                     NEWFSSRCLGGAGAIAATAHIRTDLFVAFDRALARGDLAAGRALWRQLKPLAVGAFDE
                     PSPGPVKALLAARGECANELRAPLTTASAALTDRLLALAADLQATR"
     gene            877..>2204
                     /locus_tag="VIN75_02485"
     CDS             877..>2204
                     /locus_tag="VIN75_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY8973056.1"
                     /translation="MAHALASGDVQLDGGVMHATVGGTVVLDASGSTDADGDALSFSW
                     TLTAHPAGSQIATSGSGSTLTWKPDVTGVYTYDVVVTDGRGGSTTQSVSVTVDNQAPA
                     ASVTVSATFSATPQLAASQPVIVNGRVTIDASATADPDGDPVDVTFDIVAPAGSTATL
                     SVAGRTARFSPDLLGTYVVRVHGTDGRGASFESDYPFDASDRAPISTVAVTPTFTATP
                     LQMPSVPVTVGANIGLDATATTDPDGDPVTVTFELTQPASSTAALSVNGRAAHFSPDV
                     LGAYEVRVRGIDGRGASFETRYPFDANNRAPSPVTVATVTPVVADAGSTVVTTSVGYD
                     VLIDSGASSDPDVADGYLVRNWVLASVPAGSQATLQGAATATSVGLSPDMLGDYVVHL
                     TVTDGQGAQSMRTVIVRANNRRPVAQVTTNATPQSLPSAPSFRVPLGTQVT"
BASE COUNT          299 a          828 c          774 g          303 t
ORIGIN      
        1 gtgctgcgcc gcgccgacgc cgccatggcc cacgcgccgg ccgcgttcat ggtcacggcc
       61 ccgccgtacc tgcggccgtc gcaggacgcg ctgcgtgcgt tcttcacgac gatcgccgac
      121 gcgctgcccg cgccgctggt cgtctacgac atccccgcgc gcaccggcgt gcgaatcgag
      181 ctcgacacgc tgctcgcgct ggccgcgcat ccgaacatcg tgggcctgaa ggactgcggc
      241 gggcgcgtgg agcagacgga ggcgctgatc gccgacggcc ggttgcaggt gctgtgcggc
      301 aacgacaacg agtggttctc gtcgcgttgc ctcggcggcg ccggcgccat cgcggcgacg
      361 gcgcacatcc gcaccgacct gttcgtcgcg ttcgaccgcg cgctggcgcg gggcgacctg
      421 gccgcggggc gcgcgctgtg gcgccagctc aagccgctgg cagtcggcgc cttcgacgag
      481 cccagcccgg gcccggtcaa ggccctgctg gccgcgcggg gcgagtgcgc gaacgagctg
      541 cgggcgccgc tgacgacggc gtcggcggcg ttgacggatc gcctgctggc gctggcggcg
      601 gatcttcagg ccacgcgctg aacccggcgc ctcctcccca ggaaagggtt aatcccgagc
      661 gctcgcggcg ccgggggcgc ctgcgtttgc ccgacaatcg tccggccgct cgcgacgtgc
      721 ggccggcgtg atccgtgcga cgacccggag cgccccaaag ggaggaatgc catgtcgaga
      781 tccgcgcgcc tgtgggcgcc attcgtgttg tcctgcctgc tcgccgcctg cggtggcggc
      841 gggggatcca gccctgcaaa gcctgaccaa gcgccggtgg cccatgccct ggcctcgggc
      901 gacgtgcagc tggatggcgg cgtcatgcac gccaccgtcg gcggcacggt ggtgctggac
      961 gcgtcgggca gtaccgacgc cgacggcgat gcgctcagtt tctcgtggac gctgaccgcg
     1021 catccggccg gtagccagat cgccaccagc ggcagcggct cgaccctcac ctggaagccc
     1081 gacgtgacgg gggtctacac gtacgacgtc gtcgtcaccg atggcagggg cggttcgacg
     1141 acccagtcgg tgtcggtcac ggtcgacaac caggctccgg cggcctcggt caccgtgagc
     1201 gccaccttca gcgcgacgcc tcagctggcc gcgagccagc cggtgatcgt caacggcaga
     1261 gtgacgatcg atgcgtcggc caccgccgac cccgacggcg atccggtcga cgtcaccttc
     1321 gacatcgtcg ctccggcggg cagtaccgcg acgctgtcgg tggccggccg cacggcccgc
     1381 ttcagtcccg acctgctggg cacctatgtc gtgcgcgttc acggcaccga cggccgcggc
     1441 gcctcgttcg agtccgacta cccgttcgac gccagcgacc gcgcgccgat cagcaccgtg
     1501 gccgtgacgc ccaccttcac cgccacgccg ctgcagatgc cgtcggtgcc ggtcacggtc
     1561 ggcgccaaca tcgggctcga cgccaccgcc acgaccgatc cggacggcga cccggtcacc
     1621 gtcaccttcg agctgacgca acccgcgagc agcaccgctg cgctgtccgt caacggccgc
     1681 gcggcgcatt tctcacccga cgtgctcggc gcgtacgagg tgcgcgtgcg cggcatcgac
     1741 ggccgcggcg cctcgttcga gacgcgctac ccgttcgacg cgaacaaccg cgcgccgtcg
     1801 ccggtcacgg tcgccacggt cacccccgtg gtcgccgacg cggggtcgac ggtggtgacg
     1861 acgtcggtcg gctatgacgt gctgatcgac agcggcgcca gcagcgaccc ggatgtcgcc
     1921 gacggctatc tcgtccgcaa ctgggtgctc gccagcgtgc cggccggcag ccaggcaacg
     1981 ctgcaaggcg cggccacggc aacgagcgta ggcctgtcgc ccgacatgct gggcgactac
     2041 gtggtgcatc tgaccgttac cgacggccag ggcgcgcaat cgatgcgcac cgtgatcgtc
     2101 cgcgcgaaca accggcgccc cgtggcccag gtgaccacca acgccacgcc gcaatcgttg
     2161 ccgagcgcgc cgagcttccg cgtgccgctg ggcacccagg tgac
//
LOCUS       DATCRO010000094         9128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_124761, whole genome shotgun sequence.
ACCESSION   DATCRO010000094 DATCRO010000000
VERSION     DATCRO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124761"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VIN75_02490"
     CDS             complement(<1..2123)
                     /locus_tag="VIN75_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY8973057.1"
                     /translation="MKFLFVLMGAFAGAVLGGVPAVLAWILVGGAAGWWFFGQQRQPA
                     SPASDLDAPTLLRRVMALEREVSALRRTVADLHAASTVDAAMPQAAPAMADTPAPAAQ
                     ARPVERPAMPEPMRAPTPAVARDTVPQAAVPALPAAMATPATASVRPTPPATPAPMAA
                     PLRRPVAAPAPAVPLRDQLPPWAARWIFGGNTIVKAGVLILFLGLAFLLRYTAERVTV
                     PIELRYAGVALVGVVLTAIGWRLRDRRDAAGRNDYGVILQGAGIGIFYLTTLAALRLD
                     PLLSPDLAFAFMALVAVFGALLAVLQDAPWLAFVSVAEGFAAPALVSTGSDAYVPLMS
                     YMAILDAGILAIAWFKAWRPLNLVGAVATFALAGAWAGAHYLPGDYAGVQAFLVFFFL
                     LFTITGVLFARRALALADAPDESRPLSERAADALRHVGRVDSTLTFGVPLAAFSLQYL
                     LVRDTAWGPAWSAAAFALFHILLGGALMRRGPARFALLGEAHVIVGTIFATLTVPLAL
                     QGAWTGATWAVEAAGMYWLGARQRRPYARAFALVVMLGAAVRLLAGLTLDTRDGVPLV
                     AGSTLGMAMLAAALCALDAVRRRMDAPARGTVEDVAEPVVPFAAAFAIAGLAWMTLPV
                     LWAAAATAWIAFGCASLAGRLASPTLRVASALLHLDALTAMTLSLQVTGATPAQAHAA
                     RDLAGALLIGGALLATGWLGLRDAWR"
     gene            complement(2241..2636)
                     /gene="rbsD"
                     /locus_tag="VIN75_02495"
     CDS             complement(2241..2636)
                     /gene="rbsD"
                     /locus_tag="VIN75_02495"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEY8973058.1"
                     /translation="MKRTTLLHAELSEVIARLGHGDLLVIGDAGLPIPDGPRRIDLAV
                     SAGIPRLQDVLAAVLAEMQVEGAVIAEELAPRSPDVHAELRRQLADTPVSALPHEQFK
                     AATRGARAIVRTGEFSPYANVILRAGVVF"
     gene            complement(2633..3562)
                     /gene="rbsK"
                     /locus_tag="VIN75_02500"
     CDS             complement(2633..3562)
                     /gene="rbsK"
                     /locus_tag="VIN75_02500"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY8973059.1"
                     /translation="MSSSIVVVGSLNMDLVMRMARAPIGGETVPGLDFAMLPGGKGAN
                     QAVACARMGANVIMVGRVGIDPNGDVLKAGLAADGVDAREVKGLASTHTGVASIWVED
                     DGQNRIVLSPGANGLLDPASVARAHTMIDMAGMVLLQLEVPMAAVQATVDRAYAAHVP
                     VLLNPAPAAPLPDDMWPRIDILVLNESEATAYAQLDVVDPNTAAQAGSLLRARGPDRV
                     IVTLGAQGVVVVDGAGARHTPAKKVKAIDTTAAGDTFIGALAAALCEGQSLDDAVALG
                     QSAAAICVTRRGAQTSIPYRRQVVPSTPGVNTP"
     gene            complement(3604..4653)
                     /locus_tag="VIN75_02505"
     CDS             complement(3604..4653)
                     /locus_tag="VIN75_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8973060.1"
                     /translation="MTNATHRGHAALPTGRASIRDVAARAGVSVTTVSHTLNGTRFVS
                     EQAKAKVHEAAHALGYVPSEIARGLKHNTTRTLGMLVPNNSNPYFAEIIRGVEHHSYA
                     AGFSLLLCNSNDDPQQQADHLRVLAERRVDGIVLVASGHDDAIVAVCKDLRLPLVLVD
                     LEIESIAVDLIEVDHAAGGDLATEHLLGLGHQRVACIVGPSDLRPSHQREAGWRRALG
                     KAGIEPRPDECVRGDFGPESGAAAMRRLLHSPQRPTAVFVCNDMMAIGALHAAHEAGI
                     SVPDQMSIVGFDDIELAAYTSPPLTTVAQPKEAIGTGAAGLLLERLREGRVEPRRTVL
                     QPALHLRASTAPPRA"
     gene            complement(4739..5713)
                     /locus_tag="VIN75_02510"
     CDS             complement(4739..5713)
                     /locus_tag="VIN75_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8973061.1"
                     /translation="MTSSKPLTTRAALTSAIGTVAGLLAALALMIGFFSWQSEYFFTA
                     DTFVTIANDIPATAVTAVGMTFVLIIAGIDLSVGSTMALAAAAAGIAMLQWHWSLFPA
                     TLLAMATGVVVGAINGAVTVAWRLPSFIVTLGMLEMARGAAYLATDSRTQYIGGRIDW
                     LGKPIVLGLSPQFFIAIAIVAVAQVVLTRTVFGRSLVGIGTNEEAMRLAGVDPRPIKV
                     AVFALAGLLAGLGGVFLSSRLEAADPNAGTGAELVVIASVVIGGTSLMGGRGSVASTF
                     FGVLVISVLEAGLAQIGASEPTKRIITGFVIILAVVLDTLRERRKKNV"
     gene            complement(5710..7269)
                     /locus_tag="VIN75_02515"
     CDS             complement(5710..7269)
                     /locus_tag="VIN75_02515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY8973062.1"
                     /translation="MSPAPGGTGATPALLQGRGIGKTYATPVLAGVDFELRAGEVLAL
                     TGENGAGKSTLSKIVAGLVAPTAGTLALAGQPYAPASRQDAEKLGVRMVMQELSLVPT
                     LSVAENLLLGRLPHRLGFIRGDALNEAAARQMALIGLDDVDPRLPVSALGVGYQQMVE
                     IARSLVGDCRLLILDEPTATLTSREIAHLFEQIALLKARGVAIVYISHRLDELQQVAD
                     RVMVLRDGRHIDTRPMRELTQADIVRSMVGRDVHEGLERAVRPRGRRALAVRGLGRGN
                     VVRDVSFDLHAGEVLGLAGLVGSGRTELLRLIAGADRKDAGEVLLGEAGTPAKISSPM
                     QAVRQGIGFVTEDRKSQGLLLPQSIRVNATLANLPAVSRAGWLRRDDERAEVQRLREL
                     LRVRSESIEQPVAELSGGNQQKVVFARWLHRDCDVLLLDEPTRGVDIGARADIYAQMD
                     KLAAAGKALLMVSSDLRELMSQCDRIGVMSAGRLVRIFERGQWTEQLLLEAAFSAYIA
                     AEEQAPQQQPA"
     gene            complement(7278..8228)
                     /locus_tag="VIN75_02520"
     CDS             complement(7278..8228)
                     /locus_tag="VIN75_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY8973063.1"
                     /translation="MIRSSRRLALRALAGAAVLALLPQFALAADAKPRVALVMKSLAN
                     EFFQTMQDGAKADQKKRSGEYTLIANGIKDETDTAAQIKIVQQMLVEHVDAIVIAPAD
                     SKALVPVLAEAAAKGVIVVNIDNKLDADALKEKNLVVPFVGPDNRKGAKLAGDYLAKQ
                     LKSGDKVGILEGVSTTFNAQQRTAGFTDAMKAVGANIVSVQSGQWEMDKANAVAAAML
                     REHPDLRALLAGNDNMALGAVAAIKSAGKTGKVLVVGYDNISAIKPMLADGRVLATVD
                     QYGAKQAVFGIDTALKAIKDKKTQKDLPAVVETQVTLVTK"
     gene            8704..>9128
                     /locus_tag="VIN75_02525"
     CDS             8704..>9128
                     /locus_tag="VIN75_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8973064.1"
                     /translation="MKYTLGAIALALAAPAAFAQSSVTIYGIVDLDGQYLSGHAKQVL
                     VTSGGQSGSRIGFKGSEDLGSGYFADFVLEGGLNVDTGGSGQGGALFGRQAFGALRTP
                     FGTASAGRQYSSIYTQTGDFSEFANTSIGATTAVIGGFAG"
BASE COUNT         1325 a         3216 c         3346 g         1241 t
ORIGIN      
        1 cgccatgcat cgcgcagccc cagccagccg gtggccagga gcgcaccgcc gatcagcagc
       61 gcgccggcca ggtcgcgcgc ggcatgcgcc tgcgcgggcg tggcgccggt gacctgcagc
      121 gacagcgtca tcgccgtcaa cgcgtccagg tgcagcaacg cgctggccac ccgcagcgtc
      181 ggcgacgcga ggcggccggc gagcgacgcg cagccgaagg cgatccaggc cgtggccgcg
      241 gcggcccaca gcacgggcag cgtcatccag gccagtcccg cgatcgcgaa cgcggcggcg
      301 aagggcacga ccggctcggc gacgtcctcg accgtgccgc gcgccggagc gtccatgcgg
      361 cggcgcacgg cgtcgagcgc gcacagcgcg gccgcgagca tcgccatgcc cagcgtcgat
      421 ccggcgacca gcggcacgcc gtcgcgcgtg tccagcgtca ggcccgcgag cagtcgcacg
      481 gccgcgccga gcatcaccac cagcgcgaag gcacgcgcat acggccggcg ctggcgcgcg
      541 cccagccagt acatgccggc ggcctcgacg gcccaggtcg cgcccgtcca tgcgccctgc
      601 agcgcgagcg gcacggtgag cgtggcgaag atggtgccca cgatcacgtg cgcctcgccc
      661 agcagcgcga agcgcgccgg gccgcggcgc atcagcgcgc cgcccagcag gatgtggaac
      721 agcgcgaatg ccgcggccga ccacgccggc ccccaggccg tgtcgcgcac cagcaggtac
      781 tgcagcgaga acgccgccag cggcacgccg aaggtcagcg tgctgtcgac gcggcccacg
      841 tggcgcagcg cgtcggcggc gcgttcggac agcggacgtg actcgtcggg ggcgtctgcg
      901 agcgcgagcg cgcggcgcgc gaacagcacg ccggtgatgg tgaacagcag gaagaagaag
      961 acgaggaagg cctgcacgcc cgcgtagtcg cccggcaggt agtgcgcgcc ggcccaggcg
     1021 cccgcgagcg cgaaggtggc gacggcgccg acgaggttca gcgggcgcca cgccttgaac
     1081 cacgcgatgg ccaggatgcc ggcgtccagg atggccatgt acgacatcag cggaacgtag
     1141 gcgtcgctgc cggtgctcac cagcgcgggc gcggcgaagc cctcggccac cgacacgaag
     1201 gcgagccacg gcgcgtcctg cagcacggcc agcagcgcgc cgaacacggc caccagcgcc
     1261 atgaacgcga acgcaaggtc gggcgacagc agcggatcaa ggcgcagcgc ggccagcgtc
     1321 gtcaggtaga agatgccgat gcccgcgccc tgcaggatga cgccgtagtc gttgcggcct
     1381 gcggcgtcgc ggcgatcgcg caggcgccag ccgatggcgg tcaggaccac gccgaccagc
     1441 gccacgccgg cgtagcgcag ctcgatgggc accgtcaccc gttcggccgt gtagcgcagc
     1501 aggaaggcca ggcccaggaa caggatcagc acgccggcct tgacgatggt gttgccgccg
     1561 aagatccagc gcgcggccca cgggggcaac tggtcgcgca gcggcacggc tggtgccggg
     1621 gcggcgacgg gtcggcgcag cggcgcggcc atgggggccg gcgtggcagg cggcgtcggc
     1681 cgcaccgagg ccgtcgcggg cgtggccatc gctgccggaa gggccggaac ggcggcctgc
     1741 gggacggtat cgcgcgccac cgcgggcgtc ggggcgcgca tcggctcggg catcgccggc
     1801 cgttcgaccg ggcgcgcctg ggcagcgggc gcgggcgtgt ccgccatcgc cggcgccgcc
     1861 tgcggcatgg cagcgtcgac ggtggacgcg gcgtgcaggt ccgcaaccgt ccggcgcagc
     1921 gcactcacct cgcgctccag cgccatcacg cgccgcagca gcgtgggcgc gtcgaggtcg
     1981 gacgcgggcg aggccggttg gcgttgctgc ccgaagaacc accatccggc ggcgccgccg
     2041 acgaggatcc acgccaggac ggcgggcacg cctcccagca cggcgccggc gaaggcgccc
     2101 atcaagacga acaggaactt caagcgtgct cctcgcaggg ccaggtcgcg aggattatcg
     2161 aagctgtcga gcagccgtcc gaccacccgt gcgggggtat cgccaggcgc tccccccggc
     2221 agcgtccggc ggcccggccg tcagaacacc acgccggccc gcaggatcac gttggcgtac
     2281 ggcgagaact cgcccgtgcg cacgatcgcc cgcgcgccgc gcgtggccgc cttgaactgc
     2341 tcgtgcggca gcgcggacac cggcgtgtcg gccaactggc gccgcaactc ggcgtggacg
     2401 tccgggctgc gcggcgcgag ctcctcggcg atgaccgcgc cctcgacctg catctcggcc
     2461 agcaccgcgg ccagcacgtc ctgcaagcgc gggatgccgg cgctgacggc caggtcgatg
     2521 cggcgcgggc cgtcggggat cggcaggccg gcgtcgccga tcaccagcag gtcgccgtgg
     2581 cccagccggg cgatgacctc ggacagctcg gcgtgcagca gcgtggtgcg cttcatgggg
     2641 tgttgactcc gggcgtggac ggcaccacct ggcggcggta agggatcgac gtctgcgcgc
     2701 cgcggcgcgt cacgcagatc gcggcagcgg actgcccgag cgcgacggcg tcgtccagcg
     2761 actgcccctc gcacagcgcg gcggccagcg cgccgatgaa ggtgtcgccg gcggcggtgg
     2821 tgtcgatcgc cttgaccttc ttggccggcg tatggcgggc gccggccccg tcgaccacga
     2881 cgacgccctg cgcgcccagc gtcacgatca cgcggtcggg gccgcgcgcg cgcagcaggc
     2941 tgccggcctg ggcggcggtg ttcgggtcga cgacatcgag ctgcgcgtag gcggtggcct
     3001 cgctctcgtt gagcacgagg atgtcgatgc ggggccacat gtcgtcgggc agcggcgcgg
     3061 ccggcgccgg gttcagcagc accggcacgt gcgccgcgta cgcgcggtcc acggtggcct
     3121 gcacggcggc catgggcact tccagctgca gcagcaccat gccggccatg tcgatcatcg
     3181 tgtgggcgcg tgccacgctg gcggggtcga gcaggccgtt ggcgccgggc gacaggacga
     3241 tgcggttctg gccgtcgtcc tcgacccaga tcgaggccac gcccgtgtgc gtggatgcca
     3301 ggcccttgac ctcgcgcgcg tcgaccccgt cggcggccag gccggccttg agcacgtccc
     3361 cgttgggatc gatgccgacg cggccgacca tgatgacgtt ggcgcccatg cgcgcgcagg
     3421 ccacggcctg gttcgcgccc ttgccgccgg gcagcatcgc gaaatcgagg ccgggcacgg
     3481 tctcgccgcc gatgggcgcg cgcgccatgc gcatgaccag gtccatgttg aggctgccga
     3541 cgacgacgat ggaggagctc atgggcggac ctctcgggga cggatatggg aaggaatcat
     3601 ggctcaggcg cgcggcgggg cggtggatgc ccgcaggtgc agtgcgggtt ggaggacggt
     3661 gcggcgcggc tcgacgcggc cttcgcgcag gcgctcgagc agcagcccgg cggcgccggt
     3721 gccgatcgct tccttgggct gcgcgacggt ggtcagcggc ggcgaggtgt aggcggccag
     3781 ctcgatgtcg tcgaagccga cgatggacat ctggtcgggc acgctgatgc cggcctcgtg
     3841 cgcggcatgc agcgcgccga tggccatcat gtcgttgcac acgaacacgg cggtggggcg
     3901 ctgcggcgaa tgcagcaggc ggcgcatcgc ggcggcgccg ctctcggggc cgaagtcgcc
     3961 gcgcacgcat tcgtcggggc gcggctcgat gcccgccttg ccgagcgcgc gccgccagcc
     4021 ggcctcgcgc tggtggctcg gtcgcaggtc ggacggcccg acgatgcagg cgacgcgctg
     4081 gtggcccagg cccagcaggt gctcggtggc cagatcgccg ccggcggcgt ggtcgacctc
     4141 gatcaggtcg acggcgatcg actcgatctc gaggtcgacc agcaccagcg gcaggcgcag
     4201 gtccttgcag acggcgacga tggcgtcgtc atggcccgag gccaccagca cgatgccgtc
     4261 gacgcggcgc tcggccagca cgcgaaggtg gtcggcctgc tgctgcgggt cgtcgttcga
     4321 gttgcacagc agcaggctga agccggccgc gtagctgtgg tgctcgacgc cgcggatgat
     4381 ctcggcgaag tagggattgg agttgttggg caccagcatg cccagcgtgc gcgtcgtgtt
     4441 gtgcttgagc ccgcgcgcga tctcgctggg cacgtagccg agcgcatgcg cggcttcgtg
     4501 caccttggcc ttggcctgct cgctgacgaa gcgcgtgccg ttgagcgtgt gcgagacggt
     4561 cgtgacggac acgcccgcgc gcgccgccac gtcgcggatg ctggcgcgac cggtgggcag
     4621 ggcggcgtgg ccgcggtggg tggcgtttgt catctggaac ctcgggaagc gaggatcacc
     4681 atccgagccg atccgagctc cccctcgggg ggcagcgacg ggaggagcga gggggccgtc
     4741 atacgttctt cttcctgcgt tcgcgcagcg tgtcgagcac caccgcgagg atgatgacga
     4801 agccggtgat gatgcgcttg gtcggctcgc tcgcgccgat ctgcgccagg ccggcttcca
     4861 gcaccgagat caccagcacg ccgaagaacg tgctcgcgac ggagccgcgg ccgcccatca
     4921 ggctggtgcc gccgatgacc accgacgcga tcaccaccag ctcggcgccg gtgcccgcgt
     4981 tcgggtcggc ggcctccagg cgcgaggaca ggaacacgcc gcccaggccc gcgagcagtc
     5041 cggccagcgc gaacacggcg accttgatcg gccgcgggtc gacgcccgcc agacgcatcg
     5101 cctcctcgtt ggtgccgatg cccaccaggc tgcggccgaa cacggtgcgc gtgagcacca
     5161 cctgggcgac cgcgacgatc gcgatggcga tgaagaactg cggcgacagg cccagcacga
     5221 tgggcttgcc gagccagtcg atgcgcccgc cgatgtactg cgtgcgcgaa tcggtcgcga
     5281 ggtaggcggc gccgcgcgcc atctccagca tgcccagggt gacgatgaac gacggcagcc
     5341 gccaggccac cgtcaccgcg ccgttgatcg cgccgacgac cacgcccgtg gccatcgcca
     5401 gcagcgtggc gggaaacagc gaccagtgcc attgcagcat cgcgatgccc gcggccgcgg
     5461 cggccagcgc catcgtcgag cccaccgaca ggtcgatgcc cgcgatgatc agcacgaagg
     5521 tcatgccgac ggccgtgacc gccgtggcgg ggatgtcgtt ggcgatcgtg acgaaggtgt
     5581 cggcggtgaa gaagtactcg ctctgccagc tgaagaagcc gatcatgagc gccagcgcgg
     5641 ccagcaggcc ggcgacggtg ccgatcgcgc tggtgagcgc cgcgcgcgtg gtgagtggtt
     5701 tggacgacgt catgcgggtt gttgttgcgg ggcctgttcc tcggcggcga tataggcgct
     5761 gaacgcggcc tcgagcagca gctgctccgt ccactgcccg cgttcgaaga ttcgcaccag
     5821 cctgccggcg ctcatcacgc cgatgcggtc gcactgcgac atcagctcgc gcaggtccga
     5881 cgacaccatc agcaacgcct tgccggcggc cgcgagcttg tccatctgcg cgtagatgtc
     5941 ggcgcgggcg ccgatgtcga cgccgcgcgt gggttcgtcc agcagcagca cgtcgcagtc
     6001 gcggtgcagc cagcgtgcga acaccacctt ctgctggttg ccgcccgaga gctcggccac
     6061 cggctgctcg atcgattcgc tgcgcacgcg cagcagctcg cgcaggcgct gcacctcggc
     6121 gcgctcgtcg tcgcggcgca gccagccggc gcgcgacacc gccggcaggt tggccagcgt
     6181 cgcgttgacg cggatcgact gcggcagcag caggccctgc gacttgcgat cctcggtgac
     6241 gaagccgatg ccctggcgca cagcctgcat cggagaagaa atcttcgcgg gcgtcccggc
     6301 ttcgcccagc agcacctcgc ccgcatcctt gcggtcggcg ccggctatca gccgaagcag
     6361 ttcggtgcgg cccgaaccga cgagcccggc caggcccagc acctcgccgg catgcaggtc
     6421 gaacgagacg tcgcgcacga cgttgccgcg cccgagcccg cgcaccgcga gggcgcgccg
     6481 cccgcgcggg cgcacggcgc gctccaggcc ctcgtgcacg tcgcggccga ccatgctgcg
     6541 cacgatgtcg gcctgcgtga gctcgcgcat cgggcgcgtg tcgatgtggc ggccgtcgcg
     6601 cagcaccatc acgcggtcgg ccacctgctg cagctcgtcg agccggtgcg agatgtagac
     6661 gatggccacg ccgcgcgcct tgagcagcgc gatctgctcg aacaggtgcg cgatctcgcg
     6721 cgaggtcagc gtcgcggtgg gctcgtccag gatcagcagc cggcagtcgc ccaccaggct
     6781 gcgcgcgatc tccaccatct gctggtagcc gacgcccagc gcgctgacgg gcaggcgcgg
     6841 gtcgacgtcg tccagcccga tcagcgccat ctgccgggcg gccgcctcgt tgagcgcgtc
     6901 gccgcggatg aagcccaggc ggtgcggcag gcggcccagc agcaggttct cggcgacgct
     6961 gagcgtgggc acgaggctca gctcctgcat caccatgcgc acgcccagct tctcggcgtc
     7021 ctggcgcgag gccggcgcat agggctggcc cgcgagcgcc agcgtgccgg ccgttggcgc
     7081 gacgagtccg gcgacgatct tggacagcgt gctcttgccc gcgccgttct cgccggtgag
     7141 cgccagcacc tcgccggcgc gcagctcgaa gtccacgccg gcgagcaccg gcgtggcgta
     7201 ggtcttgccg atgccgcggc cctgcagcag cgcgggcgtc gcgcccgttc cgcccggggc
     7261 cggcgacatc gcggcggtca cttggtgacc agcgtgacct gcgtctcgac gaccgccggc
     7321 aggtccttct gcgtcttctt gtccttgatc gccttgagcg ccgtgtcgat gccgaacacg
     7381 gcctgcttgg cgccgtactg gtcgacggtg gccagcacgc ggccgtcggc cagcatcggc
     7441 ttgatggcgc tgatgttgtc gtagccgacc accagcacct tgcccgtctt gcccgcgctc
     7501 ttgatcgcgg cgacggcgcc cagcgccatg ttgtcgttgc cggccagcag cgcgcgcagg
     7561 tcgggatgct cgcgcagcat cgcggcggcg accgcgttgg ccttgtccat ctcccactgc
     7621 cccgattgca cgctgacgat gttggcgccg acggccttca tcgcgtcggt gaagcccgcg
     7681 gtgcgctgct gcgcgttgaa ggtggtggac acgccctcga ggatgccgac cttgtcgccg
     7741 gacttgagct gcttggccag gtagtcgccg gccagcttcg cgcccttgcg gttgtcgggg
     7801 ccgacgaacg gcacgaccag gttcttctcc ttgagcgcgt cggcatcgag cttgttgtcg
     7861 atgttgacga cgatcacgcc cttggccgcg gcctcggcca gcaccggcac cagcgccttg
     7921 gagtcggccg gcgcgatgac gatcgcgtcg acgtgctcga cgagcatctg ctgcacgatc
     7981 ttgatctgcg ccgcggtgtc ggtctcgtcc ttgatgccgt tggcgatcag cgtgtactcg
     8041 cccgagcgct tcttctggtc ggccttggcg ccgtcctgca tcgtctggaa gaactcgttg
     8101 gcgagcgact tcatcaccag cgcgacgcgc ggcttggcgt cggcggcgag ggcgaactgg
     8161 ggcagcagcg ccaggacggc ggcaccggcg agggcgcgca gcgccaggcg gcgggacgaa
     8221 cggatcatgg gtgtgtctcc tgatttttgg gccgcgccgg cggagggttc gggccggagg
     8281 aagccggttg gcatcgatcg cgcaaacgtt tgcgcaaacg attgcggcgg actttacgcc
     8341 cgcggtcgcg ctgccgcaag gccgggctca tccgggtttt cgcggaggcc tggcgcctga
     8401 ttgaccccgc cgcgggtgcg gaatgggcca tttcgttgct tgatagcgac gtttcgaagg
     8461 aacaaaaaaa caagttgtca atcaggtgtc acacctgatg agcaccatgg ccgggctggt
     8521 caaaacggca caaagctcaa tgaattcata agcttaggaa cgattcgtcg aggctgaacg
     8581 ctggctgtgg ctgatcgcag atcccacaat tcgcgtcggt cgacgtcgct gtcgagccgt
     8641 gagaacaagt tcaaatgccg gccggcctcc ctccggccaa ccccatcaca gaaagaacat
     8701 cacatgaagt acaccctcgg cgccatcgcc ctggctctcg ccgcccccgc cgcgttcgcg
     8761 cagtcgagcg tcaccatcta cggcatcgtc gacctcgacg gccagtacct gtccggccac
     8821 gccaagcagg tgctggtcac ctccggcggc cagtccggca gccgcattgg cttcaagggc
     8881 agcgaggatc tgggctcggg ctactttgcc gacttcgtgc tcgaaggcgg cctgaacgtg
     8941 gacaccggcg gctccggcca gggcggggcg ctgttcggcc gccaggcctt cggcgcgctg
     9001 cgcaccccgt tcggcaccgc cagcgcgggc cgccagtaca gcagcatcta cacccagacg
     9061 ggcgacttca gcgaattcgc caacaccagc atcggtgcga ccacggccgt catcggcggc
     9121 ttcgccgg
//
LOCUS       DATCRO010000095         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_237197, whole genome shotgun sequence.
ACCESSION   DATCRO010000095 DATCRO010000000
VERSION     DATCRO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237197"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VIN75_02530"
     CDS             complement(<1..409)
                     /locus_tag="VIN75_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY8973065.1"
                     /translation="MNSRLTSPRALAIALASAGVIGAVGAGAYTSARAVGAPTTNAVA
                     TAPAAMVTLPDFSTITSRDGPAVVNISVTGTMKASDDEAAAEIQGVDPDDPMFQFFRR
                     FQGQMNPRGQQQQREVPVRAQGSGFIVDPSGIII"
     gene            complement(511..1851)
                     /locus_tag="VIN75_02535"
     CDS             complement(511..1851)
                     /locus_tag="VIN75_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973066.1"
                     /translation="MRRLFGSLTGSLRARLLWFLLAAIVLAAGAQALVAYRTVLAEAD
                     DIFDYHMQQMALSLRAGLPPSAAVGGPGAAEQNFDFVVQVWTADGVRIFESAEQAALP
                     QLAVLGFADVNARGTTYRVFSMQTRGLVIQVAQDMAARRHMAGTLALRTIAPVALMAP
                     LLMLVVWWVVSRSLAPVARVRRQVASRQADDLSPVSDENLPDEVRPLVQELNLLFDRV
                     RHAFDAQKHFVADAAHELRSPLAALKLQVQGLQRAPDDASRELAVSRLVAGIDRATRL
                     VEQMLALARHEASIAAGARPEPVDLAEVARLAVSDAVAAAQARGIDIGIAHADEAAVV
                     LGQSEALRMLLRNLLENAIKYTPVEGRVDIGIARVGGTVELTVDDSGPGLPPEERERV
                     LDRFYRSGESQAPGSGLGLAIVKSIADLHGATVALDQSSSLGGLRVLVRFPARA"
     gene            complement(1961..2620)
                     /locus_tag="VIN75_02540"
     CDS             complement(1961..2620)
                     /locus_tag="VIN75_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973067.1"
                     /translation="MRLLLLEDDVMIGEAVLDLLRAEQYAVDWVKDGEAAETALRTQQ
                     YDLVMLDLGVPRRDGLEVLRNLRARKQRMPVLIATARDSVQQRIEGLDAGADDYVLKP
                     YDLDELLARIRALLRRAAGRAEPVYEHMGVSINPATREVTVAGQPVVLSAREWAVLEP
                     LLARPGMVLSRAQLEEKLYSWKDEISSNAVEVYVHGLRKKLGAEFIRNVRGVGYMVPK
                     L"
     gene            complement(2684..>3285)
                     /locus_tag="VIN75_02545"
     CDS             complement(2684..>3285)
                     /locus_tag="VIN75_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8973068.1"
                     /translation="LETDGSYFWMAPKVLRFSGSYLASSRLPRALQPTLNRLAAQTGE
                     SFSAVVLDGEEVVIVARSGNYGTPTRVLAYGLHLGARLPAHATSTGRVLLAAMAPTQF
                     TQWLKGRHLARLTPRTVTQARGLRQLIARTRKDDYCFASEEHELGVQALAVPLRDMQG
                     RTVAALNVVLSGTRYQEETLLREMLPLLFEAAREVRSLL"
BASE COUNT          499 a         1185 c         1131 g          470 t
ORIGIN      
        1 tgatgatgat gccgctgggg tcgacgatga agcccgagcc ctgcgcacgc accggcacct
       61 cgcgctgctg ttgctggccg cgcggattca tctggccctg gaagcggcgg aagaactgga
      121 acatcggatc gtccggatcg acgccctgga tctcggcggc agcctcgtcg tcggacgcct
      181 tcatggtgcc cgtcacgctg atgttgacca cggctgggcc gtcgcgcgaa gtgatcgtcg
      241 agaagtcggg cagcgtgacc atggcggccg gcgcggtcgc gacggcgttg gtggtcggcg
      301 cacccacggc gcgggcgctg gtgtaggcgc cggcaccgac ggcaccgatc acgccggcgc
      361 ttgccagggc gatggccagg gcacggggag aagtcagacg tgagttcatt cgtcggttcc
      421 tttcaaggcg tatggacgca aaacgatgaa aggaatgtcg cgggtctgac ttaagcgccg
      481 cttaaacggc gcggcgccgc ggcaagggac tcaggcgcgc gccggaaagc gcaccagcac
      541 gcgcaggccg ccgaggctgg acgactggtc gagcgccacc gtcgccccgt gcaggtcggc
      601 gatcgacttg acgatggcca gccccaaccc gctgcccggc gcctgcgact cgccggagcg
      661 gtagaaccgg tcgagcaccc gctcgcgctc ctcgggcggc aggcccggcc cgctgtcgtc
      721 cacggtgagc tcgacggtgc cgcccacgcg ggcgatgccg atgtcgaccc ggccctcgac
      781 cggcgtgtac ttgatggcgt tttccagcag gttgcgaagc agcattcgca gggcctccga
      841 ctgtccaagc accaccgcag cctcgtcggc atgcgcgatg ccgatgtcga tgcccctcgc
      901 ctgcgcagcc gccaccgcgt cggacaccgc gagccgcgcg acctcggcca ggtcgaccgg
      961 ctcgggcctg gcgccggccg cgatgctggc ctcgtgccgg gccagcgcga gcatctgctc
     1021 gaccagccgc gtcgcgcggt cgatgcccgc caccagccgg ctcacggcga gttcgcgcga
     1081 ggcatcgtcg ggtgcgcgct gcaggccctg cacctgcagc ttgagcgcgg ccaggggcga
     1141 gcgcagttcg tgcgcggcat cggcgacgaa atgcttctgc gcatcgaagg catggcgcac
     1201 gcggtcgaag agcaggttca gctcctgcac cagcgggcgc acctcgtcgg gcaggttctc
     1261 gtcgctcacc ggggagagat cgtccgcctg ccgcgaggcg acctgccttc gcacgcgcgc
     1321 caccggcgca agcgacctgc tcaccaccca ccacacgacc agcatcagaa gcggcgccat
     1381 cagcgccacc ggcgcgatgg tgcgcagcgc cagcgtaccg gccatgtggc ggcgcgccgc
     1441 catgtcctgc gccacctgga tcaccagccc gcgcgtctgc atcgagaaca cgcgatacgt
     1501 ggtgccgcgc gcgttcacgt cggcgaagcc cagcaccgcc aactgcggca gcgcggcctg
     1561 ctcggccgat tcgaagatgc gcacgccgtc ggccgtccac acctgcacga cgaagtcgaa
     1621 gttctgctcg gcggcgcccg gccctcccac cgcggcgctc ggcggcaggc ccgcgcgcag
     1681 cgacagcgcc atctgctgca tgtgatagtc gaagatgtcg tcggcctccg cgagcaccgt
     1741 gcgataggcc accagcgcct gtgcgcccgc agccagaacg atggctgcaa gaaggaacca
     1801 caacaggcgc gcgcgcagcg aaccggtcag cgacccaaac agccgcctca tgccccctcc
     1861 cttcgcggga cgtcacggaa ccggcttcgc cggcccgtag acgtcgcccc cggaaggggg
     1921 ttggcgaagc gacacgaagt gcgcgaggcc tgggggcgag tcatagcttc ggcaccatgt
     1981 agccgacccc cctcacattg cggatgaact ccgcgccgag cttcttgcgc agcccgtgca
     2041 catagacctc gaccgcgttg ctgctgatct cgtccttcca gctgtagagc ttctcctcga
     2101 gctgcgcgcg cgacagcacc atgccgggcc gcgccagcag gggctccagc acggcccatt
     2161 cgcgcgccga cagcaccacc ggctggccgg ccaccgtgac ctcgcgcgtg gccgggttga
     2221 tgctcacgcc catgtgctcg tacaccggct ctgcgcgccc cgccgcccgg cgcagcaagg
     2281 cgcgtatgcg ggccagcagc tcgtcgaggt cgtagggctt gagcacatag tcgtcggcgc
     2341 cggcatcgag gccttcgatg cgctgctgca ccgagtcgcg cgcggtggcg atcagcaccg
     2401 gcatgcgctg cttgcgcgcg cgcaggttgc gcaagacctc gaggccgtcg cgcctgggca
     2461 cgcccaggtc gagcatcacg aggtcgtatt gctgggtccg cagcgccgtt tcggcggcct
     2521 cgccgtcttt cacccagtcc accgcgtact gttcggcgcg cagcaggtcg agcaccgctt
     2581 cgccgatcat cacgtcgtct tcgaggagga gcagtcgcat ggttgtgtcc gtttcggggg
     2641 ttcgaggcgc gggcttcggc caaagttcag atcgggcccg cgctcagagc agagaccgga
     2701 cttcgcgcgc ggcttcgaag agcagcggca gcatctcgcg cagcagtgtc tcctcctggt
     2761 agcgcgtgcc cgagagcacc acgttgagcg ccgccacagt gcggccctgc atgtcgcgca
     2821 gcggcacggc cagcgcctgc acacccagct cgtgttcctc gctggcgaag cagtagtcgt
     2881 ccttgcgcgt gcgggcgatc agctgccgca ggccccgtgc ctgcgtcacc gtgcggggcg
     2941 tgagccgcgc caggtgccgg cccttgagcc attgcgtgaa ctgggtcggc gccatcgccg
     3001 ccagcagcac gcgcccggtg gaggtggcat gcgcgggcag ccgcgcgccc aggtgcaggc
     3061 cgtaggcgag cacccgggtc ggcgtgccgt agttgccgct gcgcgcaacg atgacgacct
     3121 cctcgccatc gagcaccacg gccgagaacg actcgccggt ctgcgccgcg agccggttca
     3181 gcgtcggctg cagcgcgcgc ggcaggcgcg acgaggcgag atagctgccc gagaagcgca
     3241 gcaccttcgg cgccatccag aaatagctgc cgtcggtctc caggt
//
LOCUS       DATCRO010000096         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_46069, whole genome shotgun sequence.
ACCESSION   DATCRO010000096 DATCRO010000000
VERSION     DATCRO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46069"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VIN75_02550"
     CDS             complement(<1..361)
                     /locus_tag="VIN75_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8973069.1"
                     /translation="MPAIVSALNVKSDAFAANAARMADRLAEVRRLERLVVEESASKR
                     DKFERRGQLLPRERVAQLLDRGSGFLELSPLAGLGMHDDDGRKSVQGGGSIVGIGLVS
                     GKRVVISASDSALKGGTV"
     gene            complement(361..1278)
                     /locus_tag="VIN75_02555"
     CDS             complement(361..1278)
                     /locus_tag="VIN75_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973070.1"
                     /translation="MTTDSPDQNAAAIPSSRYRSVFAPGLFAGRRHWVTGGGSGIGRA
                     VAHELASLGAEVVISGRSADKLERVAAEIAEDGGRCTWRAFDIRDDEAVRGNVADVVA
                     KEGVVHGLVNNAGGQFPAPMMAISKRGFEAVVANNLTGGFLMMRELFLQSFQAHGGAI
                     VNMTADFRNGMPGMAHSGAARAGMSNLTMTASFEWASSGVRVNAVAPGWIASSGMDTY
                     GGAMKTLIPQLKRHVPLRRMGLEAEVSAAIVFLLSPAAAFITGVTLQIDGGASLGSPL
                     FPSVDHDRSVPFDGFHRAVTPRVLREGEG"
     gene            1486..1785
                     /locus_tag="VIN75_02560"
     CDS             1486..1785
                     /locus_tag="VIN75_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8973071.1"
                     /translation="MDIQTATDQLRAKVGEDCGLGAVLKFDCGADGAVVIDATKVPNG
                     VSNDADRVADCTVALGVDTLGDLLSGELEPTTGFMMGKFKVSGDMSVALKLQRIV"
     gene            1794..2252
                     /locus_tag="VIN75_02565"
     CDS             1794..2252
                     /locus_tag="VIN75_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8973072.1"
                     /translation="MESQASKAARGLRAQTADEVVADHRSDDAGQLFGITELCKEFDI
                     SPRALRFYEDKGLLAPRRVNGTRVYTRRDRARLQLILRAKAIGSSLAEIKHYLDLYGA
                     HGEGRAVQMKYVLDKTSKAIAELEQKRAHIDASLAELRIINASVRRSLGK"
BASE COUNT          373 a          835 c          787 g          321 t
ORIGIN      
        1 agacggtgcc gcccttgagc gcgctgtcgc tcgccgagat gacgacgcgc ttgcccgaca
       61 ccagcccgat gcccacgatg gagccgccgc cctgcaccga cttcctcccg tcgtcgtcgt
      121 gcatgcccag gcccgccagc ggcgacaact cgaggaatcc gctgccccga tcgagcagct
      181 gcgcgacgcg ctcgcgcggc agcagctggc cgcgccgctc gaacttgtcg cgcttcgacg
      241 cgctctcctc gacgaccagc cgctcgagcc ggcgcacttc ggccagcctg tcggccatgc
      301 gcgccgcgtt ggccgcgaac gcgtcggact tcacgttgag cgccgagacg atcgccggca
      361 tcagccctct ccctcgcgca gcacccgggg cgtgaccgcg cgatggaagc cgtcgaacgg
      421 caccgagcgg tcgtggtcga ccgacgggaa cagcggcgag cccagcgacg cgccgccgtc
      481 gatctgcagc gtgacgccgg tgatgaacgc ggccgccggc gacagcagga acacgatggc
      541 cgcgctcacc tcggcctcga ggcccatgcg ccgcagcggc acgtggcgct tgagctgcgg
      601 gatcagcgtc ttcatcgcgc cgccgtaggt gtccatgccg ctggaggcga tccagcccgg
      661 cgcgaccgcg ttgacgcgca cgccggacga cgcccactcg aaggaggccg tcatcgtgag
      721 gttgctcatg cccgcgcgcg ccgcgcccga gtgcgccatg cccggcatgc cgttgcggaa
      781 gtcggcggtc atgttgacga tggcgccgcc gtgcgcctgg aagctctgca ggaagagctc
      841 gcgcatcatc aggaagccgc cggtcaggtt gttggcgacg acggcctcga agccgcgctt
      901 gctgatggcc atcatcggcg ccgggaactg gccgccggcg ttgttgacca ggccgtggac
      961 gacgccctcc ttcgcgacga cgtcggcgac gttgccccgg acggcctcgt cgtcgcggat
     1021 gtcgaacgcg cgccaggtgc agcggccgcc gtcctcggcg atctcggcgg cgacgcgctc
     1081 gagcttgtcg gcgctgcggc cgctgatgac cacctcggcg cccagcgacg ccagttcgtg
     1141 cgcgaccgcg cggccgatgc cggagccgcc gccggtgacc cagtggcgcc ggccggcgaa
     1201 cagcccgggc gcgaacacgc tgcgatagcg cgaagacggt attgcggcag cgttctggtc
     1261 gggagagtct gtcgtcatgg ggacacgatc gtttccggag gaaaggggct ggcccgtcgg
     1321 cacgtgccgc attaggcttc agggtgacgt taacgtcaac atcatgtcgc gtcaggaatt
     1381 tcactgatgt cgacgtcacg tgcgggccct agcatggcgg cgatcgcccg gcgcgccatc
     1441 gcgcgccttc gccagccatc cactcctgca ccaggaaccc atcgcatgga catccagacc
     1501 gccaccgacc aactgcgcgc caaggtgggc gaggactgcg gcctcggcgc cgtgctcaag
     1561 ttcgactgcg gcgccgacgg cgccgtcgtg atcgacgcca ccaaggtccc caacggcgtg
     1621 tccaacgacg ccgaccgcgt ggccgactgc accgtcgcac tcggcgtcga caccctcggc
     1681 gacctgttgt cgggcgaact ggagcccacc accggcttca tgatgggcaa gttcaaggtc
     1741 tcgggcgaca tgagcgtcgc gctgaagctg caacgcatcg tctgagcatc gtcgtggaat
     1801 cgcaggcatc gaaagccgcg cgcggcctgc gcgcgcagac cgccgacgag gtcgtcgccg
     1861 accaccgcag cgacgacgcc ggccagttgt tcggcatcac cgagctctgc aaggagttcg
     1921 acatctcgcc gcgcgcgctg cgcttctacg aggacaaggg gctgctcgcg ccgcgccgcg
     1981 tcaacggcac gcgcgtctac acgcggcgcg accgcgcgcg gctgcagctg atcctgcgcg
     2041 ccaaggccat cggctcctcg ctggccgaga taaagcacta cctcgacctg tacggcgccc
     2101 acggggaagg ccgcgccgtg cagatgaagt acgtgctcga caagacctcc aaggcgatcg
     2161 ccgagctcga gcagaagcgc gcgcacatcg atgcgtcgct cgcggagctg cgcatcatca
     2221 atgcgagcgt caggcgcagc ctgggcaagt aggcgcgcgt cgaacgagcg gccggcggca
     2281 accaacggga tcccgaatcg aaagtcagga catgac
//
LOCUS       DATCRO010000097         6421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1013529, whole genome shotgun sequence.
ACCESSION   DATCRO010000097 DATCRO010000000
VERSION     DATCRO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013529"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VIN75_02570"
     CDS             complement(<1..665)
                     /locus_tag="VIN75_02570"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8973073.1"
                     /translation="MPPPTAPDLEPPRGLADTLFRGAQVALVNVAIAGLVTTMTGNAF
                     GHVVLYAECIGLSIWAIVEMGRRRSPGAPRGWPPGWRGPALVAVACALGYVIGVTVAD
                     TLLGYSSWPRYLHAPGQLARDFGPTVVFCTVVAGAFWARGQARVHRAQVAAAAHEATL
                     ARLDMLQSQLEPHMLFNTLANLRALIAADPPRAQEMLDHLIAFLRATLAASRQSEHPL
                     SEE"
     gene            complement(705..1223)
                     /locus_tag="VIN75_02575"
     CDS             complement(705..1223)
                     /locus_tag="VIN75_02575"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEY8973074.1"
                     /translation="MNTLIAILEHTPTAVWAVLAVLVLVGLRQTRTQTLSGGRVWLVP
                     LIAGVASLAGALRGFGAAGELLTGACWAIGAGLGLAANRALDLPRRVVANADGSFTIG
                     GSIAPLFLLVGIFLVRYATNVALAVQPSLANDPLAAAVVAIAYGLTAGLLAARARKIW
                     STRADQGAAQAA"
     gene            1679..2440
                     /locus_tag="VIN75_02580"
     CDS             1679..2440
                     /locus_tag="VIN75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY8973075.1"
                     /translation="MSFSFVIESWAACAPGVDTPQRWAQWASAPWLPEGEPQVALAAV
                     APMLRRRFAPLGRLAAQAALDLAGSAAVEPGADLDDQPTVYASRYGETARCLELLADQ
                     ARGEALSPTSFGLSVHNAIGAMIALARGDRRNSSAIASGRVTVAAGVAEALALLEDGA
                     ASVNLVAYDSPLPAGYARFEDEPAAHFAFAWKLRRPRAGERALHLEWAQADGEAEPAQ
                     PQLPSGLRALWFGLSDARTLTQADGGRRCTWSRDD"
     gene            2433..3251
                     /locus_tag="VIN75_02585"
     CDS             2433..3251
                     /locus_tag="VIN75_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8973076.1"
                     /translation="MTEATMAAAPAPARGSLANRWWRVAGTGFSFALFGLGGLLLGLV
                     VFPLLLVAVRQPRRRSAIARSVIHHLFRAFVALMCGLGLCRLEVRGRERLQRRGLLIL
                     ASHPSLIDVVFLMSLVRDADCIVKAALARNPFTRGPVLAAGYVFNDSGPGLIDDCVAS
                     VRSGNNLIVFPEGTRTPADMALGRLQRGAANLAVRGALAITPVRITCEPRMLGKGIKW
                     WRVPPTAGNFVIEIGEDMPIADHLAEEGAQALAARRLNDELARRLGAETPHAAA"
     gene            3238..3498
                     /locus_tag="VIN75_02590"
     CDS             3238..3498
                     /locus_tag="VIN75_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY8973077.1"
                     /translation="MQQLENDIKELIISSLALEDITPADIDASAPLFVDGLGLDSIDA
                     LELGLALQKKYGVSLSAESEEVRAHFASVKALAAFVVAQGKA"
     gene            3510..3767
                     /locus_tag="VIN75_02595"
     CDS             3510..3767
                     /locus_tag="VIN75_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8973078.1"
                     /translation="MTKDDIFARIVEILNQTFDIEPARITRESRLGEDLDIDSIDAVD
                     LIVQLKPLVGKRLQPEAFKSVRTVQDVVDALYGLVHEAPAA"
     gene            3788..4405
                     /locus_tag="VIN75_02600"
     CDS             3788..4405
                     /locus_tag="VIN75_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973079.1"
                     /translation="MPTPAAPARKTPPLLAAVLLAVSLAYPVVVYLALGHVSPRWIAL
                     LLVVLALARAWVTRESFWLGAAALATLLAAASFLGDFWGPLKLYPALVNAVMFGLFAM
                     SLWRGPSVVERLARLREPDFPPAAVAYTRRVTQVWCGFFVVNGLVAVATALWASTAVW
                     TLYNGLLSYVAMGALMGGEWLVRQRVRARIAAQDAARARPGASNA"
     gene            4398..6233
                     /locus_tag="VIN75_02605"
     CDS             4398..6233
                     /locus_tag="VIN75_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8973080.1"
                     /translation="MRDAGWHPLTELPRTTPATSSTVAWRGGEALSAADFAAQAAAWR
                     DLLAARPGARWALFTEDTFDFACALFGAWHAGKTVVLPGDMQDDTVARLHGVADGFLG
                     DLPGALACPAAARTGGVRSFEPLDRAATQLVVYTSGTNGEPLAIAKTLAQLDAEVHAL
                     EARFGALCDTGLAPVNLACESMAGLQDPAADVETPVVWATVTHQHIYGLLFRVLWPLA
                     AGRPFAAGRLVFNEEIAQRVHGPAVLVASPAHLRRLPEAVDWSAARAGLRAVFSSGGP
                     LPPEAADATLALLGVAPVEVYGSSETGGIAWRQRARHGDTWQPLPGIEFRVEDGALAV
                     RSPHLPDLEWFHTADRASLAADGMDGFELHGRADRIVKIEEKRVSLTALEQRLAGSGD
                     VAEARVLMLADERPVVDAAGVALPHAVRPAAVVALSEAGRAKLARIGKPALVAELRAL
                     LLAAVDRVALPRRWRLVDALPVNAQGKSTEALLGTLFAPAVDERPLLPQPRWRIAEAT
                     HALASIALDPALKVFDGHFPGTPILPGVAQVHWAIAFARGRFDMPPRFVRIDALKFSQ
                     PATPGMHLDLELRWNADAATLQFEYRSTSGRHSSGRIVLAAAEGA"
     gene            6233..>6421
                     /locus_tag="VIN75_02610"
     CDS             6233..>6421
                     /locus_tag="VIN75_02610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8973081.1"
                     /translation="MASPIRTCAVIPVYNHGQAVGAVAASVRANGLHCFLVDDGSSAD
                     CAAVLDALAQAPDVTLVRL"
BASE COUNT          798 a         2335 c         2423 g          865 t
ORIGIN      
        1 aattcctccg acagcggatg ttcggactgc cggctcgcgg ccagcgtggc gcgcaggaac
       61 gcgatcaggt ggtcgagcat ctcctgggcg cgtggcggat ccgcggcgat cagcgcgcgc
      121 aggttggcca gcgtgttgaa cagcatgtgc ggctcgagct gcgactgcag catgtccagc
      181 cgcgccagcg tggcctcgtg cgcggccgcg gccacctgcg cgcggtgcac gcgcgcctgc
      241 ccgcgggccc agaacgcgcc ggccacgacg gtgcagaaga cgacggtcgg cccgaagtcg
      301 cgcgccagct gccccggcgc gtgcaggtac ctgggccagc tggagtagcc caagagggtg
      361 tcggcgacgg tgacgccgat cacgtagccc agggcgcagg ccacggccac cagtgccggg
      421 ccgcgccagc cgggcggcca gccccgtggc gcgccggggc tgcggcggcg acccatctcg
      481 acgatcgccc agatcgacag cccgatgcac tccgcgtaca gcacgacatg gccgaacgcg
      541 ttgcccgtca tcgtggtgac cagtcccgcg atggccacgt tgacgagggc cacctgggcg
      601 ccgcggaaca gcgtgtcggc aaggccgcgc ggtggctcga ggtcgggggc ggtcggaggc
      661 ggcacggcag gcggggtcgc ggaggcaagg cggggattgt cgcctcaggc cgcctgcgcg
      721 gcgccctggt cggcgcgggt cgaccagatc ttgcgcgcgc gggccgccag caggccggcg
      781 gtcaggccgt aggcgatggc gaccaccgcc gcggccagcg ggtcgttggc cagcgacggc
      841 tggacggcca gcgcgacgtt ggtggcgtag cgcaccagga agatgcccac cagcaggaac
      901 aacggcgcga tgctgccgcc gatcgtgaag ctgccgtcgg cgttggccac gacgcggcgc
      961 ggcaggtcca gcgcgcggtt cgcggccaag ccgaggccgg cgccgatcgc ccagcaggcg
     1021 ccggtgagca gctcgccggc cgcgccgaag ccgcgcaggg cgccggccag cgacgcgacg
     1081 ccggcgatca gcggcaccag ccagacccgg ccgcccgaca gcgtctgggt gcgcgtctgg
     1141 cgcaggccga ccagcacgag cacggccagg acggcccaga cggcggtggg ggtgtgctcg
     1201 aggatggcga tcagggtgtt catggcggtg tccttcgcga ggggaggcgg gttggaatgc
     1261 ttcacatggt cgccgcggcg ccggcggccg gccagcggcg agcgacggga cacccgccgg
     1321 cggcgcgaaa cgacgcccgc gcggcgcggg atgcggcggc ctggacgggc gccgtgcgcc
     1381 gctgtcgtgc agcgagcctg gggagaaccc tcgggctgtc cgtcaggcac ctgtcgatcc
     1441 ggcgtgaaat gcgccgcgcc accccgtgaa accgagagtc tcgacaagac ttccccccag
     1501 gaagcaagct tggccacagg ctaaaatgag ccgttggaag gggaggccgc gatgcgtacc
     1561 cgccgggggg ttccggcagg cggtgcagcg gacctggagg tcacgcggca gacagccgcg
     1621 ggcaacacga aaaacggcgc cgccacacgc ggcgaacgtc ggcgtcaggg ctagccaatt
     1681 gagtttttct ttcgtcatcg agtcctgggc ggcgtgcgcg cccggcgtgg acacgccgca
     1741 gcgctgggcg cagtgggcga gcgcgccctg gctgcccgag ggcgagccgc aggtcgcgct
     1801 ggccgcggtc gccccgatgc tgcgccgccg cttcgcgccg ctggggcgcc tggccgcgca
     1861 ggcggcgctc gacctcgcgg gttcggctgc cgtcgagccc ggcgccgacc tcgacgacca
     1921 gcccaccgtc tacgcatcgc gctacggcga gaccgcgcgc tgcctcgagc tgctggccga
     1981 ccaggcgcgc ggcgaggccc tgtcgcccac gtccttcgga ctgtccgtgc acaacgcgat
     2041 cggggccatg atcgcgctgg cccgcggcga ccgccggaac tcgtcggcca tcgcgtccgg
     2101 gcgcgtcacc gtcgcggccg gcgtggccga ggcgctggcc ctgctggagg acggcgcggc
     2161 cagcgtcaac ctcgtcgcct acgactcgcc gctgcccgcc ggctatgcga ggttcgagga
     2221 cgagccggcc gcccatttcg cgttcgcgtg gaagctgcgc cgaccgcgcg ccggcgagcg
     2281 cgcgctgcac ctggaatggg cgcaggccga cggcgaggcc gagcccgcgc aaccgcaact
     2341 gccgtccggg ctgcgggcgc tgtggttcgg gctgtccgac gcgcgcacgc tgacgcaggc
     2401 cgacggcggc cgccgctgca cctggagccg cgatgactga ggcgacgatg gcggccgcgc
     2461 cggcgcccgc gcgcggctcg ctggccaatc gttggtggcg cgtggcgggc accggcttct
     2521 cgttcgcgct gttcgggctc ggcgggctgc tgctgggcct ggtcgtgttc ccgctgctgc
     2581 tggtcgccgt gcgccagccg cgacgccgct ccgcgatcgc gcgcagcgtc atccatcacc
     2641 tgttccgcgc gttcgtcgcc ctgatgtgcg gcctggggct gtgccggctc gaggtgcgcg
     2701 gccgcgagcg gctgcagcgg cgcgggctgc tgatcctggc cagccatccg tcgctgatcg
     2761 acgtggtgtt cctgatgtcg ctggtgcgcg atgccgactg catcgtcaag gccgcgctgg
     2821 cgcgcaaccc gttcacgcgc gggccggtgc tggccgcggg ctacgtgttc aacgattccg
     2881 gcccggggct gatcgacgac tgcgtcgcgt cggtgcgctc gggcaacaac ctgatcgtgt
     2941 tccccgaggg cacgcgcacg cccgccgaca tggcgctcgg ccggctgcag cgcggcgcgg
     3001 ccaacctcgc ggtgcgcggc gcgctggcca tcacgccggt gcgcatcacc tgcgaaccgc
     3061 gcatgctcgg caagggcatc aagtggtggc gcgtgccgcc caccgcgggc aacttcgtga
     3121 tcgagatcgg cgaggacatg ccgatcgccg accacctggc cgaggagggc gcgcaggcgc
     3181 tcgccgcgcg ccgactcaac gacgaactgg cgcgccgact cggcgcggag actccccatg
     3241 cagcagcttg agaacgacat caaggaactc atcatctcct ccctcgcgct ggaggacatc
     3301 acgcccgcgg acatcgacgc ctcggcgccg ctgttcgtcg acggcctggg cctggattcc
     3361 atcgacgcgc tggagctcgg cctcgcgctg cagaagaagt acggcgtgag cctgtcggcc
     3421 gaatccgagg aggtgcgcgc ccatttcgcg agcgtcaagg cgctggcggc cttcgtggtc
     3481 gcgcagggca aggcgtgaag ggagacgaca tgaccaagga cgacatcttc gccaggatcg
     3541 tggagatcct gaaccagacc ttcgacatcg agccggcgcg catcacgcgc gagtcgcgcc
     3601 tcggcgagga cctcgacatc gactcgatcg acgcggtcga cctcatcgtg cagctcaagc
     3661 ccctggtggg caagcgcctg cagcccgagg ccttcaagtc ggtgcgcacg gtgcaggacg
     3721 tcgtcgacgc gctgtacggc ctcgtgcacg aggcgccggc ggcgtgaact gcgcgatggc
     3781 gccggccatg ccgactccgg ccgctcccgc gcgcaagacg ccgccgctgc tggctgcggt
     3841 gctgctcgcc gtgagcctgg cctatcccgt ggtggtgtat ctcgcgctgg gccatgtcag
     3901 cccgcgctgg atcgcgctgc tgctggtggt gctggcgctc gcgcgcgcct gggtcacgcg
     3961 cgagtcgttc tggctcgggg ccgccgcgct cgcgacgctg ctcgccgcgg ccagtttcct
     4021 gggcgatttc tgggggccgc tgaagctcta tcccgcgctc gtcaacgccg tgatgttcgg
     4081 cctgttcgcg atgagcctgt ggcgcgggcc cagcgtcgtc gagcgcctcg cgcgcctgcg
     4141 cgaacccgac ttcccgcctg ccgccgtcgc ctacacgcgc cgcgtcacgc aggtgtggtg
     4201 cggcttcttc gtggtcaacg gcctcgtcgc ggtggccacc gcgctgtggg ccagcaccgc
     4261 cgtctggacg ctgtacaacg gcctgctgtc gtacgtcgcg atgggcgcgc tgatgggcgg
     4321 cgaatggctg gtgcgccagc gcgtgcgcgc ccgcatcgcg gcgcaggacg cggcccgcgc
     4381 gcgccccgga gcctcgaatg cgtgatgccg gctggcaccc gctgaccgag ctgccccgca
     4441 ccaccccagc gacctcgagc acggtggcct ggcgcggcgg cgaggccctg tcggcggccg
     4501 atttcgcggc gcaggcagcc gcctggcgcg acctgctggc cgcccggccc ggcgcgcgct
     4561 gggcgctgtt cacggaagac accttcgact tcgcctgcgc gctgttcggc gcctggcatg
     4621 ccggcaagac ggtggtgctg ccgggcgaca tgcaggacga caccgtcgca cgcctgcatg
     4681 gcgtcgccga cggcttcctt ggcgacctgc cgggcgcgct cgcgtgcccc gcggcggcgc
     4741 gcacgggagg cgtgcgatcg ttcgagccgc tggatcgcgc ggcgacgcag ctggtcgtct
     4801 acacctcggg caccaacggc gagccgctgg ccatcgccaa gacgctcgcg cagctggacg
     4861 ccgaagtcca tgcgctggag gcgcgtttcg gcgcgctgtg cgacaccggc ctcgcgccgg
     4921 tcaacctcgc ctgcgagtcg atggcgggcc tgcaggatcc ggccgccgac gtcgagacgc
     4981 ccgtggtctg ggccacggtc acgcaccagc acatctacgg cctgctgttc cgcgtgctgt
     5041 ggccgctggc cgccggccgg ccgttcgcgg cggggcggct cgtgttcaac gaggagatcg
     5101 cgcagcgcgt ccacgggccc gcggtgctgg tggccagccc cgcgcacctg cgccggctgc
     5161 ccgaggcggt cgactggtcg gccgcgcgcg ccgggctgcg cgcggtattc tcgtccggcg
     5221 gcccgctgcc gcccgaggcc gccgacgcga cgctcgcgct gctgggcgtg gcgccggtcg
     5281 aggtctacgg cagctccgag accggcggca ttgcgtggcg ccagcgcgcg cgccatggcg
     5341 acacctggca gccgctgccc ggcatcgagt tccgcgtcga ggacggcgcg ctcgccgtgc
     5401 gctcgccgca cctgcccgac ctcgagtggt tccacacggc cgatcgcgcg agcctggcgg
     5461 ccgacggcat ggacggcttc gagctgcacg gccgcgccga ccgcatcgtc aagatcgagg
     5521 agaagcgcgt gtcgctgacc gcgctggagc agcgcctggc cggcagcggc gacgtcgccg
     5581 aggcgcgcgt cctgatgctg gccgacgagc ggcccgtcgt cgacgccgcg ggcgtcgcgc
     5641 tgccccacgc ggtgcggccg gcggccgtcg tcgcgctgag cgaggccggg cgcgcgaagc
     5701 tggcgcgcat cggcaagccg gcgctggtcg cggagctgcg ggcgctgctg ctggcggcgg
     5761 tcgaccgcgt cgcgctgccg cgccgctggc gcctcgtcga cgcgctgccc gtgaacgcgc
     5821 agggcaagag caccgaggcg ctgctgggga cgctgttcgc gccggccgtg gacgaacggc
     5881 cgctgctgcc gcagcccagg tggcgcatcg ccgaggccac gcatgcgctg gcctcgatcg
     5941 cgctcgatcc ggcgctgaag gtcttcgacg ggcatttccc cggcacgccc atcctgcccg
     6001 gcgtcgcgca ggtgcactgg gcgatcgcgt tcgcgcgcgg gcgtttcgac atgccgccgc
     6061 gcttcgtccg catcgacgcg ctcaagttct cgcagcccgc gacgccgggc atgcacctgg
     6121 acctcgagct gcgctggaac gcggacgcgg cgacgctgca gttcgaatac cgctcgacgt
     6181 cgggccggca ttcgagcggg cgcatcgtgc ttgccgccgc ggagggcgcg tgatggcctc
     6241 gccgatccgc acctgcgcgg tgatcccggt ctacaaccat gggcaggccg tgggcgccgt
     6301 cgcggccagc gtgcgcgcga acggcctgca ctgcttcctc gtcgacgacg gcagctcggc
     6361 cgactgcgcc gcggtgctgg acgcgctggc gcaggcgccc gacgtcacgc tggtgcggct
     6421 c
//
LOCUS       DATCRO010000098         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_574861, whole genome shotgun sequence.
ACCESSION   DATCRO010000098 DATCRO010000000
VERSION     DATCRO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574861"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VIN75_02615"
     CDS             <1..307
                     /locus_tag="VIN75_02615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY8973082.1"
                     /translation="PGPDDYFVLKPKHSAFFATPPEILLDHLRTRRLTVTGVTADQCV
                     LNTAADARMRDFEVVVPRDCIASLSAARTRRAIEHLHDALDAKVPASSALRLPRRRA"
     gene            complement(316..870)
                     /locus_tag="VIN75_02620"
     CDS             complement(316..870)
                     /locus_tag="VIN75_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY8973083.1"
                     /translation="MAESPAGGLAALDTWDDKGRLQAVIEAPRGSANKLKFDPGRGVF
                     TLHNVLPLGVAFPFDFGFVPGTLGEDGDPLDVLVLMDEPVFPGVVVPCRLIGVIEARQ
                     RDKAARTARSKRNDRLVAVADKSHRHGDVKALDDLGDAVLSEIERFFVFYNDQKGVRF
                     TPIGRAGKAAARRLVEAGRRAAAG"
     gene            complement(876..1055)
                     /locus_tag="VIN75_02625"
     CDS             complement(876..1055)
                     /locus_tag="VIN75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEY8973084.1"
                     /translation="MSDDMKNRGPQDRARINLSEEHEVRYWTHALGVDKERLAAAVKA
                     VGSSADRVREHLKGR"
     gene            complement(1130..1534)
                     /locus_tag="VIN75_02630"
     CDS             complement(1130..1534)
                     /locus_tag="VIN75_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973085.1"
                     /translation="MEQTTSNPSTTTNEAASGEATPFPVSGQAASATAGGAADAARRH
                     DPGAAASAAPVVDNLLKRVTQSAHAAVDGVSERIASTMEGLQGGASRVGDTRDEWVES
                     AREAIRQHPLAALGGAVLVGLALHSLLSSRDR"
     gene            1679..2209
                     /locus_tag="VIN75_02635"
     CDS             1679..2209
                     /locus_tag="VIN75_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY8973086.1"
                     /translation="MDFKDLVDVFGMTMPVLELVLRGSVMYWFLVLVFRLILRRDVGS
                     MGITDFLFIVLLGDAAQNGMIGEASSATDAIALIATLVFWNVLIDWATWRFKWVERLF
                     AAQRLCLVRDGRLNRRNMRREFISDAELMSKVREEGLEQLSDVKAMYLESDGEISLIR
                     RQGPDDKPPPPPVALH"
     gene            complement(2216..>2500)
                     /locus_tag="VIN75_02640"
     CDS             complement(2216..>2500)
                     /locus_tag="VIN75_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973087.1"
                     /translation="VGRLPAGAMDDLLDLLQWPAMVVTLLASWWVASTHRRKRAWGFW
                     AYLLSNALWSAWGWHTSATALVVLQACLAAMNVRGFVKASRTRGDAGREN"
BASE COUNT          342 a          885 c          898 g          375 t
ORIGIN      
        1 gcccggaccc gacgactact tcgtcctcaa gcccaagcat tccgcattct ttgcgacgcc
       61 gccggagatc ctgctcgacc acctgcgcac gcggcgcctg accgtcaccg gcgtgacggc
      121 cgaccagtgc gtgctcaaca ccgcggccga cgcgcgcatg cgcgacttcg aggtggtggt
      181 gccgcgcgac tgcatcgcct cgctgagcgc cgcgcgaacg cgccgcgcca tcgagcacct
      241 gcacgacgcg ctggacgcga aggtgccggc atcgagcgcg ctgcggttgc cgcgccgccg
      301 tgcctgagcg cgcgctcaac ccgcggccgc acgccgtccg gcctccacca gcctgcgcgc
      361 ggcggccttg cccgcgcgcc cgatcggcgt gaagcgcacg cccttctggt cgttgtagaa
      421 gacgaagaag cgctcgatct ccgacagcac ggcgtcgccg aggtcgtcca gcgccttcac
      481 gtcgccgtgg cgatgcgact tgtcggccac cgcgacgagc cggtcgttgc gcttcgatcg
      541 cgccgtgcgg gcggccttgt cgcgctgccg cgcctcgatc acgccgatca ggcggcaggg
      601 cacgaccacg ccggggaaga cgggctcgtc catcagcacc agcacgtcca gcgggtcgcc
      661 gtcctcgccc agggtgccgg gcacgaagcc gaagtcgaac gggaacgcga cgcccagcgg
      721 cagcacgttg tgcagcgtga agacgccgcg cccgggatcg aacttgagct tgttggcgct
      781 gccccgaggc gcctcgatga cggcctgcag ccggcccttg tcgtcccagg tgtcgagcgc
      841 ggcgaggccg ccggccggcg actcggccac gtgcgtcagc ggcccttgag gtgctcgcgc
      901 acgcgatctg cgctgctgcc caccgccttg acggcggcgg ccaggcgctc cttgtcgacc
      961 ccgagcgcgt gcgtccagta gcgcacttcg tgttcttccg agaggttgat gcgcgcccgg
     1021 tcctgcgggc ctcggttctt catgtcgtcg gacatggcgt ctccgtcggg aatggtgatg
     1081 agccaggtcg gcaatcgcaa tgccggcctg gcccgcctcc cgggggcgtt cagcggtcgc
     1141 gcgacgacaa caggctatgc agcgccaggc cgacgagaac ggcaccgccg agcgccgcga
     1201 gcgggtgctg gcggatcgcc tcgcgggccg actccaccca ttcgtcgcgc gtgtcgccca
     1261 cgcgcgaggc gccgccctgc aggccttcca tcgtggaggc gatgcgctcg gagacgccgt
     1321 cgacggccgc gtgcgcgctc tgcgtcaccc gcttgagcag gttgtcgacc accggcgcgg
     1381 cggacgcggc ggcgccggga tcgtggcggc gcgccgcgtc ggcggcgccg ccggccgtgg
     1441 cgctggcggc ctggccgctg acggggaagg gcgtcgcctc gccggaagcg gcctcgttgg
     1501 tggtggtcga agggttggag gtggtctgtt ccatggtcgt tcgcaggttc gtggaaggaa
     1561 gaggggcggc ggccgcgtcc tcgcagccgg ctcgatggcg tcctcggcaa ggtgcgcgcc
     1621 cgatcgggca ccgtcgttgc ctgtcgcgca gcaccggtca cgacggggag gggatgccat
     1681 ggacttcaag gatctggtcg atgtcttcgg catgaccatg ccggtgctcg aactggtgct
     1741 gcgcggcagc gtcatgtact ggttcctggt gctggtgttc cgcctgatcc tgcggcgcga
     1801 cgtcggctcg atgggcatca cggacttcct cttcatcgtg ctgctgggcg acgccgcgca
     1861 gaacggcatg atcggcgagg cctcgtcggc caccgacgcg atcgcgctca tcgcgacgct
     1921 ggtgttctgg aacgtcctga tcgactgggc gacctggcgc ttcaagtggg tcgagcggct
     1981 gttcgcggcg cagcgcctgt gcctggtgcg cgacggccgg ctcaaccggc gcaacatgcg
     2041 gcgcgaattc atctcggacg cggaactgat gtccaaggtg cgcgaggaag ggctcgagca
     2101 gctgagcgac gtcaaggcca tgtacctgga gtcggacggc gagatcagcc tgatccgccg
     2161 gcaaggcccg gacgacaagc cgccgccgcc gccggtcgcc ctgcattgag gcgtctcagt
     2221 tctcgcggcc cgcgtcgccg cgcgtccggc tcgccttgac gaagccgcgg acgttcatcg
     2281 ccgcgaggca ggcctgcagc accacgaggg cggtcgccga cgtgtgccag ccccaggccg
     2341 accacagcgc gttgctgagc aggtaggccc agaagcccca cgcgcgcttg cggcgatgcg
     2401 tggaggcgac ccaccacgag gccagtagcg tgaccaccat cgcgggccat tgcagcaggt
     2461 cgaggagatc gtccatcgcc ccggcaggca aacgtccgac
//
LOCUS       DATCRO010000099        14292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_124803, whole genome shotgun sequence.
ACCESSION   DATCRO010000099 DATCRO010000000
VERSION     DATCRO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124803"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..398
                     /locus_tag="VIN75_02645"
     CDS             69..398
                     /locus_tag="VIN75_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973088.1"
                     /translation="MFQRCRISSRRVVAAGAQAGTRHATITGPNGASAVRTVERGDGH
                     VTDTTTGPNGRSRTRQVDRSAGSTTATVTHVNGSTTTRDTERSASGSTTTVTGPRGRT
                     GTVTVSH"
     gene            417..1244
                     /locus_tag="VIN75_02650"
     CDS             417..1244
                     /locus_tag="VIN75_02650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8973089.1"
                     /translation="MGRHLLLPRLAGGALLVLLSIVVVEALLRRYVWRRPTDLRAVAA
                     SLADALVRRGADVLGLATIAPLPAFAFAHRLTTIRMESAATWGALYLLVEFAYYWHHR
                     LTHRVRWFWASHSVHHSSNELMLASAVRLGWTGRLGGAIAFFTPLAWLGFPPQAVFGL
                     VAAGLFWQFWLHADWLPRLGPLEWVLNTPTHHRVHHASNPEYLDCNYGSSLIVFDRLF
                     GTFVALRGDIEIRYGLTTPVTSHNPVRIAFNEWLALARDVRAAHGWRARVRVMFGPP"
     gene            1680..2720
                     /locus_tag="VIN75_02655"
     CDS             1680..2720
                     /locus_tag="VIN75_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8973090.1"
                     /translation="MIRFGQSAALSGPQAVYGRDVQNGVAAAFAAANANEASTGIRFE
                     LQTLDDGGTRVKCMSNALQLLDSGTTALIGFTNGVAAEACMPMLEETGVAMLGTASGN
                     MGLRTQAAKGVYNVRAGYDAEFARIVNYLRDVGMQRLALVRLNDTSPANRDAMSAALA
                     AAGITPKASVNIDRHATDFSGVAAELMAAKPDCVLLVANAGPAAAIISHMTQARYPGL
                     FYASSYAGQDLLDTLTARHQSCVMSVVVPRPSAMGVNVVASCKRDLALLQNGDRFGIT
                     TLEGYIAGRTAVDATLAAARAGGATRGRVKEALSALRSDLGGYKVEFAGTTQGSHYVD
                     LITLDKYGRVMG"
     gene            2783..4696
                     /locus_tag="VIN75_02660"
     CDS             2783..4696
                     /locus_tag="VIN75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY8973091.1"
                     /translation="MKKSRIGIIGIGPRGLTLLERIVANERQRPSGPIEVVLFDPNVP
                     GAGCHDPAQAEFHLVNTVAGQLTLFADASVVDAGPVMEGPSFHQWLNQQSDIGAGNLI
                     GGERASPDGYYGRGLFGRYLQWAFRYVCALAPSNVEIIVKAAEVLRAQREDDAAWVLE
                     TADAKIRVDYLFLTTGHTRPARPAAEPAPGARATRVVDDPYPVQQTLESLRAGMAVAV
                     EGMGLTSFDVIAELTVGRGGRFVTDAAGKRCYVRSGREPHLLLFSRSGIPLSARAVNQ
                     KGISNQYRAKFLLLRHVRDLRATRRLDFVADVLPLLVADMEYAYCDAWLHEHRGAAAA
                     AAFCASFAAAGASQRQALVARTIPEADRFSWERLVAPVPHTALASRRSFGQWLRWHLQ
                     GDVREARQGNLGSPLKAACDVLRDLRDNLRAAIEFGALTPESHRWVLAEFMPVMNRLA
                     VGPPMSRVSEMLALMEAGVLEADFGPGAQCVAPPDGGPMRLMAAHWPGESTEVDALVR
                     ARISMHSPQDDASPLLRGLLDDGHARLFRNGDFHPGGIDIDRQHNWIGRNGEVLRNAW
                     ALGIPVEGAKWCTFVVPRPGVNSTAIVDAGRAVAAMLAAVRSGEAVRESRLPRRLRSE
                     AEASQYASLHGSP"
     gene            4693..5940
                     /locus_tag="VIN75_02665"
     CDS             4693..5940
                     /locus_tag="VIN75_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY8973092.1"
                     /translation="MIARVFNRLSLAGLCATLVGNGLGRFAFVALMPALIQAGWFSKA
                     QASTLGVATLVGYVFGAAVADSLAARWSSARLLRVSMLACSLSFLACAFEGAGMAWNL
                     LWRSAAGVAGALLMVLPAPLVLPRHAAAIRARTGGVVFSGIGLGAALSGWLVPLLVGG
                     PATVASAQGVAIAWLGLGAVCLALTAGAWRAWPAEATAPPAAVDRPPLPADLRRTVAL
                     VLLAHGLNAVGYLAHTLFLPDYVVRELGMPLAQGGFYWSMFGLGAAIGPMVAGTLADA
                     FGLRRCLVAGFALKAVSAFLPVWGGGAATLLVSAIVMGLCTPGVVVMVSAYTLEQVGP
                     SHHRRAWGLATSSFAIAQAAGGALMAFAATRLSSYQPLFCASAVALAGSVACIALVRE
                     RAAPAAAPSANRLLPVVITAPHP"
     gene            5937..7085
                     /locus_tag="VIN75_02670"
     CDS             5937..7085
                     /locus_tag="VIN75_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8973093.1"
                     /translation="MIKHLKSAALLAAAALLALACTGAGAQIRIGQTSGFTGPVAASV
                     GEINVGARLYLDHVNATGGIGGQQIELVSLDDKNQPDLTLQNATRLVADPKVVALFLN
                     RGTPHTRALMPLLAEAKVVLLAPSSGAMELHTPVNPWIFNVRATYQAETERLTRHLGM
                     TRLDKVGICYVNDSFGVDALQGALKVFGQGSTTPPVVEAIDKAKPDYTTCIRKIVENR
                     QLGAVFIGTPVSVAAGVKALRAAGSSATVATLSNNASSGFVKELGPVAQGVVVSQVFP
                     SERSLATPMIAEAARLAAAKDIKQLTPAMIEGYAAAKVLVLGLRKASSDGKATVTRAS
                     FKKALESFDQVDIGGGLGGTSLSYSPTDHTGLDYVDLSYIGGDGTFRR"
     gene            7221..8156
                     /gene="lpxC"
                     /locus_tag="VIN75_02675"
     CDS             7221..8156
                     /gene="lpxC"
                     /locus_tag="VIN75_02675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY8973094.1"
                     /translation="MLQQRTLKSITRAVGVGLHSGQKVEMTLRPAAPDTGIVFRRVDL
                     PQPVEIPANAGSVSDTRMNSTISPGGDPGSPKVQTIEHLMSACAGLGVDNLIVDMTAD
                     EVPILDGSSASFVFLLQSAGIVLQDAPKRFMRVLKPVEVRRGEGASLAWARLEPYHGF
                     KLTFEIEFNHPAVDATGQQVSFDMGSGKYKSDIARARTFGFTKDLDLMRSRGLTMGGS
                     MDNAIVVDDSRVLNADGLRYDDEFVKHKILDAIGDLFVVGHPLLASYTAYKSGHAMNN
                     MLLRALLDDPTAYELVTFEDERKAPAGFAELAPAW"
     gene            8257..8718
                     /locus_tag="VIN75_02680"
     CDS             8257..8718
                     /locus_tag="VIN75_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973095.1"
                     /translation="MSLRCFIVRRCLHASLEAVGLRAVNLPMVVDARRHVHPPVPLDA
                     GYLVVDRRAFARITFPRADGTQDATMQTVGFASLSGVLRAICAERIGLAGRAAIPGPK
                     RALELRREGRWSEVLEASERVEAPMRARLESWLRSWRFATWRFALQAFKRF"
     gene            9070..10278
                     /locus_tag="VIN75_02685"
     CDS             9070..10278
                     /locus_tag="VIN75_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8973096.1"
                     /translation="MPQFLAARFLRPNPGTASVLAALLVLGACSDKAPPAGPPPAPEV
                     GVVTVAPRTLDVPTELPGRVEAARVAQVRARVTGIVQKRLFAEGSDVKAGEALFQIDP
                     APYRAALDSAKASLAKATANEQQATGLLNRYKPLREANAISQQDFVNATSAAAQAGAD
                     IASAKAAVQTAQLNLDYATVTAPISGRIGQALVTEGALVSATEATQLALIQQVDQVYV
                     NFSQPVSDITKLRKAFASGRSKSLGLSIPVRIVLDDGSVLPQTGKLLFSDLTVDQTSG
                     QVNLRAKVPNPDGALMPGMYVRGRIAQEQVDSAVLLPQQAVQRTTSNDTVLVVGADGK
                     PSVRPVKLGGNENGNWIILDGLKPGEQVIVDGFQKIRPGAAVKPVPWNASAPATTAPA
                     ASAPASAASR"
     gene            10292..13444
                     /locus_tag="VIN75_02690"
     CDS             10292..13444
                     /locus_tag="VIN75_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8973097.1"
                     /translation="MAKFFIERPIFAIVIALFVLVFGAVAITQLPIAQYPPVAPPTIV
                     LNATYPGASAKTMDEAVISVIEQELNGSPGMIYMESVSQSNGTGSITVSYEPGTSPDL
                     AQVDVQNRLSRAAPRLPSAVTQQGVRVDKARSNFLLFTILSSDNPAYDPVALGDYASR
                     NVVPELQRVAGIGQAQLFGTERAMRIWVDPAKLVGYKLSMTDVTNAIAAQNAQVSAGA
                     IGDIPNVTSQGITATVIVQGQLTSVEQFGAIVLRANPDGSSVRLRDVARLELGAQNYA
                     TSARLDGQPSTGIGVQLSPTGNALATAEAIKVRMGQLSKYFPQGVKYSIPYDSSRFIK
                     ISIRQVVETLLEAVALVFVVMFVFLQDWRYTLIPTIVVPVALLGTFGVLLALGYSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSTEGLPPLEATRKAMSQIQGAIVGVTVVLI
                     SVFVPLAFFGGAVGNIYRQFSTVMVASIGFSAFMALSLTPALCGMILKPVEAGHHHAK
                     RGLFGLFNRNFEKVAHGYEGMVGRALSRVGRRFMIMLFALVVGGVVLFFFKLPASFLP
                     GEDQGYLIVNVQLPPGATRNRTLEVMKQVEGYMLKQPEVQDMVSVLGFSFSGSGQNAA
                     LAFVPLKPWDERSGAEHSAQALAGRAFGALSRVRDAFIFPLSPPPIPELGTASGFNFR
                     LQDRGGLGHDALLAARNQLLGMASKDPLLAGIRPDGLEDASQLEVTVDREKAASQGVG
                     FAAVNAAISTGFGSSYVNDFPNAGRLQRVVVQADQQSRMQPDDLLRLNAINASGQPVP
                     LSSFASVRWVTGPVQSIRYNGYPAMRIAGDASPGHSTGEAMNEMEKLAAQLPAGIGYE
                     WTGQSREEKLSGSTAPILLGFSLLAVFLCLAALYESWSIPMAVLLVVPIGVLGALLGA
                     WSRGFANDVYFKVGLITIIGLSAKNAVLIIEFAKDLQAQGKSAVEAGLEAIRLRFRPI
                     IMTSLAFMLGVLPLAISRGAGSASQRAIGTGVMGGMATATLIGVVFVPLFFVLVRSLF
                     KGSKRQHDFDLAHSSRSFRE"
     gene            13450..>14292
                     /locus_tag="VIN75_02695"
     CDS             13450..>14292
                     /locus_tag="VIN75_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8973098.1"
                     /translation="MADNLLRKPLVTKLPLRATAAAALACCLAACTSMAPRYERPAAP
                     VAAAFPAVAASAPAAAGGVAPAAQPWQAYFQDPRLAQLIGAALANNRDLRVAVLNIEV
                     ARAQVGLSRSGELPTIDLQGTHTRETGLQTVNTVGLAVAGYELDLWGRVRSLTDAALA
                     QLAATEEARKAAQISLVASVANAYYAMAGDQALLDVARQTLDTRADTLKLVQLRFDNG
                     VASSIDLQTNKSLVEGARIAFVAAQRQRALDQNALELLVGGPLPANMPAAPAWDDMAL
                     PDVPA"
BASE COUNT         1970 a         5221 c         5002 g         2099 t
ORIGIN      
        1 gatcaggatc agcggcttgg agttcatttg agccgaagtg tagggcggcg tcgcgcgcgc
       61 gaggcgcgat gtttcagcgc tgtcgcattt cgtcgcggcg cgtggtcgcg gccggcgccc
      121 aggccggcac gcgccacgcc acgatcaccg gcccgaacgg cgcgagcgcc gtgcgcaccg
      181 tcgaacgcgg cgacggccac gtcaccgaca ccaccaccgg cccgaacggc cgcagccgca
      241 cgcgccaggt cgaccgcagc gccggcagca ccacggcgac cgtcacgcat gtcaacggca
      301 gcaccacgac gcgcgacacc gagcgctcgg cctcgggctc cacgacgacg gtgaccggcc
      361 cgcgcggccg caccggcacc gtcaccgtgt cgcactgacg caaggcgcgc acgcccatgg
      421 gacgacatct gctccttccg cgcctcgcgg gcggcgcgct gctcgtgctg ctgtccatcg
      481 tggtcgtgga ggcgctgctg cgccgctacg tgtggcggcg gccgaccgac ctgcgcgccg
      541 tcgccgcgtc cctggcggac gccctggtgc ggcgcggcgc cgacgtgctg ggactggcga
      601 cgatcgcgcc gctgccggcc ttcgcgttcg cgcaccgcct gacgacgatc cgcatggagt
      661 cggccgcgac ctggggcgcg ctgtacctgc tcgtggagtt cgcctactac tggcaccacc
      721 ggctcacgca ccgcgtgcgc tggttctggg cgtcgcactc ggtgcatcac tcgtccaacg
      781 aactgatgct cgcatcggcg gtgcgcctgg gctggacggg ccgcctgggc ggcgccatcg
      841 cgttcttcac gccgctggcg tggctcggct tcccgccgca ggccgtgttc ggcctcgtcg
      901 cggccggcct gttctggcag ttctggctgc acgcggactg gttgccgcgc ctcggcccgc
      961 tcgaatgggt gctcaacacg ccgacgcacc accgggtcca tcatgcgagc aaccccgaat
     1021 acctggactg caactacggc agctcgctga tcgtgttcga ccggctgttc ggcaccttcg
     1081 tcgcgttgcg cggcgacatc gagatccgct acggcctgac cacgcccgtg acgagccaca
     1141 acccggtgcg catcgcgttc aacgaatggc tcgcgctcgc gcgtgacgtg cgcgccgcgc
     1201 acggatggcg cgcgcgcgtg cgggtgatgt tcgggccgcc ctgaagcgcg ctccgccccg
     1261 atgaggcggc gacgcattct gttcgcgcgc gcccccggaa cccgttcgca tgccggaccc
     1321 cgtgcgcctg ccaggccatt tcgacaaccc cctcaatggg gtatcgggat ggtgtgtcga
     1381 gaagtcccta cggcgcatgg attgcaagca aatgcgtgct gcatcgccgc gcatctatcg
     1441 aggtgaagat acctatacct gtatgtgtag aaaggagaag gtgcaaccaa atgcgtcttg
     1501 cgtaatggcg agcaatcttt gctaagcggc agcgccggcc aaccgaagag atgagcacgc
     1561 cgcagctcgc ggcgagcagg gagatcaata tgaagcgccg gaccttgttg cttggttgtg
     1621 gttctcttgc cggtgtcctt gccgcgccgc tcgcgcgagc cggcgcgacg gggccgcggg
     1681 tgatccgctt cggccagtcg gccgcgttgt ccggcccgca ggccgtctac gggcgcgacg
     1741 tgcagaacgg cgtcgccgcg gccttcgcgg cggccaacgc caacgaggcg agcaccggca
     1801 tccgcttcga gctgcagacg ctcgacgacg gcggcacgcg cgtcaagtgc atgtccaacg
     1861 cgctgcagct gctcgactcc ggcaccaccg cgctgatcgg cttcacgaac ggcgtggccg
     1921 ccgaggcctg catgcccatg ctcgaggaga cgggcgtcgc gatgctcggc accgccagcg
     1981 gcaacatggg cctgcgcacg caggcggcca agggcgtcta caacgtgcgc gccggctacg
     2041 acgccgagtt cgcgcgcatc gtcaactacc tgcgcgacgt cggcatgcag cgtctcgcgc
     2101 tggtgcgcct gaacgacacc tcgccggcca accgcgacgc gatgagcgcc gcactcgcgg
     2161 ccgcgggcat cacgcccaag gcctcggtca acatcgaccg gcacgccacc gacttctccg
     2221 gcgtcgccgc cgaactgatg gccgccaagc ccgactgcgt gctgctggtg gccaacgccg
     2281 gcccggcggc ggccatcatc tcccacatga cgcaggcgag gtacccgggc ctgttctacg
     2341 cctcgtcgta cgccggccag gacctgctgg acacgctcac cgcgcgccac cagagctgcg
     2401 tcatgagcgt ggtggtgccg cggccctccg cgatgggcgt caacgtcgtc gcgagctgca
     2461 agcgcgatct cgcgctgctg cagaacggcg accgcttcgg catcacgacg ctcgagggct
     2521 acatcgcggg ccgcaccgcc gtggacgcca cgctggcggc ggcgcgcgcc ggcggcgcca
     2581 cgcgcggccg cgtcaaggag gcgctgtcgg cgctgcgcag cgatctcggt ggctacaagg
     2641 tggagttcgc gggcacgacg caagggtcgc actacgtcga cctgatcacc ctcgacaagt
     2701 acggccgcgt gatgggctag ccctgcgccg cgctccgtcc ccctggacgg acgcggtcgc
     2761 gacctgagct tggagatcga cggtgaagaa gtctcgcatc ggcatcatcg ggatcggtcc
     2821 gcgcggcctg accctgctcg aacgcatcgt ggccaacgag cggcagcggc cctcgggccc
     2881 gatcgaggtc gtcctgttcg acccgaacgt gccgggcgcc ggctgccacg atccggccca
     2941 ggcggagttc cacctggtca acaccgtggc cggccagctg acgctgttcg cggacgcgtc
     3001 ggtggtcgac gccggccccg tgatggaagg cccgtccttc caccaatggc tcaaccagca
     3061 gagcgacatc ggcgcgggca acctgatcgg cggcgagcgc gcatcgccgg acggctacta
     3121 cggccgcggc ctgttcggcc gctacctgca gtgggcgttc cgctacgtgt gcgcgctggc
     3181 gccgtccaac gtcgagatca tcgtcaaggc cgccgaggtg ctgcgcgcgc agcgcgagga
     3241 cgacgcggcc tgggtgctgg agacggccga cgccaagatc cgcgtcgact acctgttcct
     3301 caccacgggc cacacgcggc ccgcgcgacc tgccgcggaa ccggcgcccg gcgcgcgcgc
     3361 gacgcgggtc gtcgacgatc cctatccggt gcagcagacc ctcgagtccc tccgcgccgg
     3421 catggccgtg gcggtcgaag gcatgggcct gacgagcttc gacgtgatcg ccgaactgac
     3481 ggtcggacgc ggcggacgct tcgtcaccga cgccgcgggc aagcgctgct acgtccgctc
     3541 gggccgcgag ccgcacctgc tgctgttctc gcgctcgggc atcccgctga gcgcgcgggc
     3601 ggtcaaccag aagggcatct ccaaccagta ccgcgcgaag ttcctgctgc tgcggcacgt
     3661 gcgcgacctg cgcgccacgc gccgcctcga cttcgtcgcc gacgtgctgc cgctgctggt
     3721 cgccgacatg gagtacgcct actgcgacgc ctggctgcac gagcaccgcg gcgccgccgc
     3781 ggcggccgcg ttctgcgcca gcttcgcggc ggccggcgcg tcgcagcgcc aggcgctcgt
     3841 ggcgcgcacc attcccgagg ccgaccgctt ctcgtgggag cgcctcgtcg cgccggtgcc
     3901 gcacaccgcg ctggccagcc gccgctcgtt cggccagtgg ctgcgctggc acctgcaggg
     3961 cgacgtgcgc gaggcgcgcc agggcaacct cggcagcccg ctgaaggccg cctgcgacgt
     4021 gctgcgcgac ctgcgcgaca acctgcgcgc ggcgatcgag ttcggcgcgc tcacgccgga
     4081 gtcgcaccgc tgggtgctgg ccgagttcat gccggtgatg aaccggctgg ccgtgggccc
     4141 gccgatgtcg cgcgtcagcg agatgctggc gctgatggag gcgggcgtcc tcgaggccga
     4201 cttcgggccc ggcgcgcaat gcgtcgcgcc gccggacggc gggccgatgc ggctcatggc
     4261 cgcgcactgg ccgggcgaat cgacggaggt ggacgcgctg gtgcgggcac gcatctcgat
     4321 gcacagcccg caggacgatg cctcgccgct gctgcgcggc ctgctggacg acggccacgc
     4381 gcgcctgttc cgcaacggcg acttccaccc gggcggcatc gacatcgacc gccagcacaa
     4441 ctggatcggc cgcaacggcg aggtcctgcg caacgcctgg gcgctgggca ttcccgtcga
     4501 gggcgccaag tggtgcacgt tcgtcgtgcc gcggccgggc gtcaattcga ccgccatcgt
     4561 cgacgcgggc cgcgcggtgg ccgcgatgct cgccgcggtg cgcagcggcg aggcggtgcg
     4621 cgagtcgcgc ctgccgcgcc gcctgcgcag cgaggccgag gcgtcgcaat acgcgtcgct
     4681 ccacgggtcg ccatgatcgc ccgggtcttc aatcggctga gcctggccgg cctgtgcgcg
     4741 acgctggtcg gcaacgggct gggccgcttc gcgttcgtcg cgttgatgcc ggcgctgatc
     4801 caggccggct ggttcagcaa ggcgcaggcg tcgacgctgg gcgtggcgac gctggtgggc
     4861 tacgtgttcg gcgccgcggt ggccgactcg ctggcggcgc gctggtcgag cgcgcggttg
     4921 ctgcgcgtat cgatgctggc gtgctcgctg tcgttcctgg cctgcgcgtt cgagggcgcg
     4981 ggcatggcgt ggaatctcct gtggcgctcc gcggccggcg tggccggcgc gctgctgatg
     5041 gtgttgccgg cgccgctggt gctgccgcgg cacgccgcgg cgatccgggc gcgcaccggc
     5101 ggcgtcgtgt tctcgggcat cgggctcggg gcggcgttgt cgggatggct ggtgccgctg
     5161 ctggtcggtg gccccgccac ggtggcctcg gcgcagggcg tggcgatcgc ctggctgggc
     5221 ctgggcgccg tgtgcctggc gctcacggcc ggcgcgtggc gagcatggcc ggccgaggcc
     5281 accgcgccgc ccgcggccgt cgaccggccg ccattgccgg ccgacctgcg gcgcaccgtc
     5341 gcgctcgtgc tgctcgcgca cgggctcaac gccgtgggct acctcgcgca cacgctgttc
     5401 ctgccggact acgtggtgcg cgagctgggc atgccgctcg cgcagggcgg gttctactgg
     5461 tcgatgttcg gcctcggtgc ggcgatcggc ccgatggtgg cgggcacgct ggccgatgcc
     5521 ttcgggctgc ggcggtgcct cgtcgccggc ttcgcgctca aggccgtcag cgcgttcctg
     5581 cccgtgtggg gcggtggcgc ggccacgctg ctggtgtcgg ccatcgtgat gggcctgtgc
     5641 acgccgggcg tcgtcgtgat ggtgtccgcg tacacgctgg agcaggtcgg cccgtcgcac
     5701 caccgccgcg catggggcct ggcgacctcg agcttcgcca tcgcgcaggc cgccggcggc
     5761 gccctgatgg cgttcgcggc cacgcgcctg tcttcgtacc aaccgctgtt ctgcgcgagc
     5821 gccgtcgcgc tggccggctc ggtggcctgc atcgcgctgg tgcgcgagcg ggccgcgccg
     5881 gcggccgccc cttccgccaa ccggttgctg ccggtcgtca tcaccgcacc ccatccatga
     5941 tcaagcacct gaaatcagcc gccctccttg ccgccgccgc gctgctggcc ctcgcctgca
     6001 cgggcgccgg cgcgcagatc cgcatcggcc agacctccgg cttcaccggg cccgtcgccg
     6061 cctccgtcgg cgagatcaac gtcggcgcca ggctgtacct ggatcacgtc aacgcgaccg
     6121 gcggcatcgg cgggcagcag atcgagctcg tgtcgctcga cgacaagaac cagccggacc
     6181 tgacgctgca gaacgcgacc aggctcgtgg ccgacccgaa ggtcgtcgcg ctgttcctga
     6241 accgcggcac gccgcacacg cgcgcgctga tgccgctgct cgccgaggcc aaggtggtgc
     6301 tgctggcgcc ctccagcggc gcgatggagc tgcacacgcc ggtcaacccg tggatcttca
     6361 acgtgcgcgc cacgtaccag gccgagaccg agcgcctgac gcgccacctg ggcatgacgc
     6421 ggctggacaa ggtcggcatc tgctacgtca acgacagctt cggcgtcgac gcgctgcagg
     6481 gcgcgctgaa ggtgttcggc cagggcagca cgacgccgcc ggtcgtcgag gcgatcgaca
     6541 aggccaagcc ggactacacg acctgcatcc gcaagatcgt ggagaaccgg cagctgggcg
     6601 ccgtgttcat cggcacgccg gtgtcggtgg ccgcgggcgt caaggcgctg cgtgccgcgg
     6661 gctcgagcgc cacggtggcc acgctgtcca acaacgcgtc ctcgggcttc gtcaaggagc
     6721 tcggcccggt ggcgcagggc gtcgtcgtga gccaggtgtt cccgtccgag cgcagcctgg
     6781 ccacgccgat gatcgcggag gccgccaggc tggccgcggc caaggacatc aagcagctca
     6841 cgcccgcgat gatcgagggc tacgccgccg ccaaggtgct ggtgctcggc ctgaggaagg
     6901 cgtccagcga cggcaaggcg accgtcacgc gcgcatcgtt caagaaggcc ctggagtcct
     6961 tcgaccaggt cgacatcggc ggcggactgg gcgggaccag cctcagctac agccccaccg
     7021 accacacagg gctggactac gtcgacctgt cgtacatcgg cggcgacggg acgttccgcc
     7081 gctgagcgcg tcgcgccggc ccgcggccgg caggggtggc gcgggcccgc ggggggcgat
     7141 cgattcgctg catcgccacg atgcgcaatc ccattgcccc ggggcgcgcg cggccggcgg
     7201 ccattgccta caattcgcgg atgctccagc aacgcacgct caagtccatc acccgcgccg
     7261 tcggcgtggg cctgcacagc ggccagaagg tggagatgac cctgcgcccg gccgcgcccg
     7321 acaccggcat cgtcttccgc cgcgtcgacc tgccccagcc ggtcgagatc cccgccaacg
     7381 ccggctcggt gtccgacacg cgcatgaact cgacgatctc cccgggcggc gaccccggca
     7441 gccccaaggt gcagaccatc gagcacctga tgtcggcctg cgccggcctg ggcgtcgaca
     7501 acctgatcgt cgacatgacg gccgacgagg tgccgatcct cgacggctcg tccgccagct
     7561 tcgtgttcct gctgcagagc gcgggcatcg tgctgcagga cgcccccaag cgcttcatgc
     7621 gcgtgctcaa gcccgtcgag gtgcgccgcg gcgagggcgc atcgctcgcc tgggcgcgcc
     7681 tggagcccta ccacggcttc aagctgacct tcgagatcga gttcaaccac ccggccgtgg
     7741 atgccaccgg ccagcaggtg agcttcgaca tgggcagcgg caagtacaag agcgacatcg
     7801 cgcgcgcccg caccttcggc ttcacgaagg acctggacct gatgcgctcg cgcggcctga
     7861 cgatgggcgg cagcatggac aacgccatcg tcgtcgacga ctcgcgcgtg ctcaacgcgg
     7921 acggcctgcg ctacgacgac gagttcgtca agcacaagat cctggacgcc atcggcgacc
     7981 tgttcgtcgt gggccacccg ctgctggcca gctacaccgc ttacaagagc ggccacgcga
     8041 tgaacaacat gctgctgcgc gcgctgctgg acgacccgac ggcctacgag ctggtcacgt
     8101 tcgaggacga gcgcaaggcg cccgccggct ttgccgaact cgcgccggcc tggtagcgcg
     8161 cgtcagcggc ccgaaggccg ccggtaacaa gccgagatgg cagcaagcgc ggccacgggc
     8221 caaaatgggt ttttgctcat ccgcctgacg gcgtccatgt ccttgcgctg cttcatcgtc
     8281 cgccgctgcc tgcacgccag cctcgaggcc gtcggcctgc gtgccgtgaa cctgcccatg
     8341 gtggtcgacg cacgccgcca cgtgcatccg cccgtgccgc tggatgccgg ctacctggtg
     8401 gtcgatcgcc gggcgttcgc ccgcatcacc ttcccgcgcg ccgacggcac ccaggacgcg
     8461 acgatgcaga cggtgggctt cgcgtcgctg tcgggcgtgc tgcgcgccat ctgcgccgag
     8521 cgcatcggcc tggccggccg cgccgccatc cccggcccga agcgcgcgct ggaactgcgc
     8581 cgcgaagggc gctggagcga ggtgctcgaa gccagcgaac gggtcgaggc gccgatgcgc
     8641 gcccggctcg agtcctggtt gcgcagctgg cgctttgcca cttggcgctt cgcgctgcag
     8701 gcgttcaagc ggttctgagc ctgcgctgat ctcccgccgg cggccaaccg gccccggccg
     8761 ggtcgcgctc gtccaatccc gaccgcggta ccggacaaca taaactagct tattattttc
     8821 ccgctcagga tccgggcggc atctccatgc ccgggaagaa ggtgccgagg ccggctggag
     8881 gagagggtgc cggtcgcggc cagggccgcc ggccgttcgc ccgtggggcg ggcgccgcgt
     8941 gccgcccctg cccccgactt ctcgcgccgc gtgacgcctg gcatggcgcg cggcctgccc
     9001 cgatcctgcg ccgcagctga cattccaagg cggactccgc cgtccaacgc atccaaagag
     9061 acaaaccgca tgccgcaatt cctggccgcc cgcttcctgc gcccgaatcc tggaaccgct
     9121 tccgtcctgg ccgccctgct ggtgctcggc gcctgcagcg acaaggcgcc gccggccggc
     9181 ccgccgccgg cgcccgaggt tggcgtcgtc accgtcgccc ccaggacgct ggacgtgccc
     9241 accgagctgc ccggccgcgt cgaggcggcg cgcgtggcgc aggtgcgcgc ccgggtgacg
     9301 ggcatcgtgc agaagcgcct gttcgccgag ggcagcgacg tcaaggccgg cgaggcgctg
     9361 ttccagatcg accctgcgcc gtaccgcgcc gcgctcgaca gcgccaaggc ctcgctcgcc
     9421 aaggccacgg ccaacgagca acaggccacc gggctgctga accgctacaa gccgctgcgc
     9481 gaggccaacg ccatcagcca gcaggatttc gtcaacgcca cgtccgccgc ggcgcaggcc
     9541 ggcgccgaca tcgcgtcggc caaggccgcc gtgcagaccg cccagctcaa cctcgactac
     9601 gccacggtca ccgcgccgat cagcggccgc atcggccagg cgctcgtcac cgagggcgcg
     9661 ctggtgagcg cgaccgaggc cacgcaactg gcgctgatcc agcaggtcga ccaggtctac
     9721 gtgaacttct cgcagccggt cagcgacatc accaagctgc gcaaggcgtt cgccagcggc
     9781 aggtccaagt cgctgggcct gtcgattccc gtgcgcatcg tgctggacga cggcagcgtg
     9841 ctgccgcaga cgggcaagct gctgttctcg gacctgaccg tcgaccagac ctcgggccag
     9901 gtcaacctgc gcgccaaggt gcccaacccg gacggtgcgc tgatgcccgg catgtacgtg
     9961 cgcggccgca tcgcgcagga gcaggtcgac tccgcggtgc tgctgccgca gcaggccgtg
    10021 cagcgcacca cgtccaacga cacggtgctg gtggtgggcg ccgacggcaa gccctcggtg
    10081 cgccccgtca agctcggcgg caacgagaac ggcaactgga tcatcctgga cggcctgaag
    10141 ccgggcgagc aggtgatcgt cgacggcttc cagaagatcc gcccgggcgc cgccgtcaag
    10201 ccggtgccgt ggaacgcgag cgcgcccgcc acgacggcgc ctgccgcgtc cgcgccggcc
    10261 tccgccgcca gccgctgatc gggcagtcga catggccaag ttcttcatcg agcgtccgat
    10321 cttcgcgatc gtcatcgcgc tgttcgtgct ggtgttcggc gcggtcgcga tcacgcagct
    10381 gccgatcgcg cagtacccgc cggtggcccc gcccaccatc gtcctgaacg ccacgtaccc
    10441 cggtgcgtcg gccaagacga tggacgaggc cgtcatctcc gtcatcgagc aggagctcaa
    10501 cggctcgccg ggcatgatct acatggagtc ggtcagccag tccaacggca ccggcagcat
    10561 caccgtcagc tacgagccgg gcaccagccc cgacctcgcg caggtggacg tgcagaaccg
    10621 gctgtcgcgc gcggcgccgc gcctgccgtc ggcggtcacg cagcagggcg tgcgcgtcga
    10681 caaggcgcgc tcgaacttcc tgctgttcac gatcctgtcc tccgacaacc cggcctacga
    10741 cccggtcgcg ctcggcgact acgcgtcgcg caacgtggtg cccgagctgc agcgggtggc
    10801 gggcatcggc caggcgcagc tgttcggcac cgagcgcgcg atgcgcatct gggtcgaccc
    10861 cgccaagctc gtcggctaca agctgtcgat gaccgacgtc accaacgcga tcgcggcgca
    10921 gaacgcgcag gtctcggcgg gcgcgatcgg cgacatcccg aacgtgacgt cgcagggcat
    10981 caccgccacc gtgatcgtgc agggccagct caccagcgtc gagcagttcg gagcgatcgt
    11041 gctgcgcgcg aaccccgacg gctcgtcggt gcgcctgcgc gacgtcgccc gcctggagct
    11101 gggcgcgcag aactacgcga cgtcggcccg cctggacggc cagccgtcca ccggcatcgg
    11161 cgtgcagctg tcgcccacgg gcaatgcgct ggccacggcc gaggcgatca aggtgcgcat
    11221 gggccagctg tccaagtact tcccgcaggg cgtcaagtac agcatcccct acgacagctc
    11281 gcgcttcatc aagatctcga tccgccaggt ggtggagacg ctgctcgagg ccgtcgcgct
    11341 cgtgttcgtc gtcatgttcg tgttcctgca ggactggcgc tacacgctga tccccaccat
    11401 cgtggtgccg gtcgcgctgc tgggcacctt cggcgtgctg ctggcgctcg gctactcgat
    11461 caacgtgctc acgatgttcg gcatggtgct ggtgatcggc atcgtggtcg acgacgccat
    11521 cgtggtcgtc gagaacgtcg agcgcatcat gagcaccgag ggcctgccgc cgctggaagc
    11581 cacgcgcaag gcgatgagcc agatccaggg cgccatcgtg ggcgtgacgg tggtgctgat
    11641 ctccgtgttc gtgccgctgg ccttcttcgg gggcgcggtg ggcaacatct accgccagtt
    11701 ctccaccgtc atggtggcgt cgatcggctt ctcggccttc atggcgctgt cgctgacgcc
    11761 ggcgctgtgc ggcatgatcc tcaagccggt cgaggcgggg caccaccacg ccaagcgcgg
    11821 cttgttcggc ctgttcaacc gcaacttcga gaaggtggcg cacggctacg aaggcatggt
    11881 cgggcgcgcg ctgtcacgcg tgggccggcg cttcatgatc atgctgttcg cgctcgtcgt
    11941 cggtggcgtg gtgctgttct tcttcaagct gccggcctcg ttcctgccgg gcgaggacca
    12001 gggctacctg atcgtcaacg tgcaattgcc gccgggcgcc acgcgcaacc gcacgctgga
    12061 ggtgatgaag caggtcgagg gctacatgct caagcagccc gaggtgcagg acatggtgag
    12121 cgtgctgggc ttcagcttct cgggctccgg ccagaacgcg gcgctggcgt tcgtgccgct
    12181 caagccgtgg gacgagcgct cgggcgccga gcactccgcg caggccctgg ccgggcgcgc
    12241 gttcggtgcg ctctcgcgcg tgcgcgacgc cttcatcttc ccgctcagcc cgccgccgat
    12301 ccccgagctg ggcaccgcgt cgggcttcaa cttccgcctg caggaccgcg gcggcctggg
    12361 ccacgacgcg ctgctggcgg cgcgcaacca gctgctgggc atggcgtcca aggatccgct
    12421 gctcgcgggc atccggccgg acggcctgga ggacgcgtcg cagctggagg tgacggtcga
    12481 ccgcgagaag gccgccagcc agggcgtggg cttcgcggcc gtcaacgcgg cgatctcgac
    12541 cggcttcggc tcgtcctacg tcaacgactt ccccaacgcc ggccgcctgc agcgcgtggt
    12601 ggtgcaggcc gaccagcagt cgcgcatgca gcccgacgac ctgctcaggc tcaacgccat
    12661 caacgccagc ggccagcccg tgccgctgtc gtcgttcgcc agcgtgcgct gggtcacggg
    12721 gccggtgcag agcatccgct acaacggcta tccggccatg cgcatcgccg gcgacgcgtc
    12781 gcccggccac agcaccggcg aggcgatgaa cgagatggag aagctcgcgg cgcagctgcc
    12841 tgcgggcatc ggctacgaat ggaccggcca gtcgcgcgag gagaagctct cgggctcgac
    12901 cgcgccgatc ctgctgggct tctcgctgct ggccgtgttc ctgtgcctgg ccgcgctgta
    12961 cgagagctgg tcgatcccga tggccgtgct gctggtggtg cccatcggcg tgctgggcgc
    13021 gctgctgggc gcgtggtcgc ggggctttgc caacgacgtg tacttcaagg tgggcctgat
    13081 cacgatcatc ggcctgtcgg ccaagaacgc ggtgctgatc atcgagttcg ccaaggacct
    13141 gcaggcgcag ggcaagagcg ccgtcgaggc cggcctcgag gcgatccggc tgcgcttccg
    13201 ccccatcatc atgacctcgc tggccttcat gctgggcgtg ctgccgctgg ccatctcgcg
    13261 cggcgccggc tcggccagcc agcgcgcgat cggcaccggc gtcatgggcg gcatggccac
    13321 ggccacgctg atcggcgtcg tcttcgtgcc gctgttcttc gtgctggtgc gctcgctgtt
    13381 caagggcagc aagcggcagc acgacttcga tctcgcgcat tcgtcgcgct cgttcaggga
    13441 gtgaacgcga tggccgacaa cctcctgcgg aaaccgctcg tgacgaaact tcccctccgc
    13501 gccacggcgg ccgccgcgct ggcgtgctgc ctggccgcgt gcacctcgat ggctccgcgc
    13561 tacgagcgcc cggccgcgcc ggtggccgcc gcgttcccgg ccgtcgccgc ctcggcgccg
    13621 gccgccgcgg gcggcgtcgc gccggcggcc cagccatggc aggcctactt ccaggatccg
    13681 cgcctcgcgc agctgatcgg cgcggcgctc gccaacaacc gcgacctgcg cgtggccgtc
    13741 ctcaacatcg aggtggcgcg cgcccaggtg ggcctgtcgc gctccggcga gctccccacc
    13801 atcgacctgc aaggcaccca cacgcgcgag accggcctgc aaacggtcaa caccgtgggc
    13861 ttggccgtgg ccggctacga gctcgacctg tggggccggg tgcgctcgct gaccgatgcc
    13921 gcgctcgcgc agctggcggc caccgaggag gcgcgcaagg cggcgcagat ctcgctcgtg
    13981 gcctcggtgg ccaacgccta ctacgcgatg gctggcgacc aggcgctgct cgacgtcgcc
    14041 aggcagacgc tggacacgcg cgccgacacg ctcaagctcg tgcagctgcg cttcgacaac
    14101 ggcgtggcct ccagcatcga cctgcagacc aacaagtcgc tggtcgaggg cgcgcgcatc
    14161 gccttcgtgg ccgcgcagcg ccagcgcgcg ctggaccaga acgccctgga gctgctggtc
    14221 ggcggcccct tgcccgcgaa catgccggcg gccccggcgt gggacgacat ggcgctgccc
    14281 gacgtgcccg cc
//
LOCUS       DATCRO010000100         8749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_226082, whole genome shotgun sequence.
ACCESSION   DATCRO010000100 DATCRO010000000
VERSION     DATCRO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226082"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1645
                     /locus_tag="VIN75_02700"
     CDS             20..1645
                     /locus_tag="VIN75_02700"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY8973099.1"
                     /translation="MYHLEGNKVTLGFVVGLDYKNPWMSPFEEMQRWKTHPAIRAHIL
                     SGAVMDPIAINELLPNWKADGGPLLQPVTGDEFLFLSETGSRSVPEWLMPAPFHNHGN
                     YVISLGNVTKWMASQAEALGVEIFPGFAAAEVLYDDKGAVKGVATGNVGVGRDGEPHD
                     GFQLGMELHGKYTIFAEGARGHLGKQVLAKYDLMAGRDPQSWAIGIKELWEVPADKAK
                     PGHVVHSAGWPVTDDTFAGGFLYHLEGNKVTLGYIVGLDYKNPWMSPFEEMQRWKTHP
                     DIRKHIEGGKRLSYGARAINNGGLQCLPKLVFPGGALVGCDAGTLNAARIKGSHAAIK
                     TGMLAAEAAFAALQAGRQGDELSAYPEAFEKSWLHTELQRTKNFKLWFKKGKLVGNLM
                     TGIEQWLMPKLGMIPPWTLHGTRKDNEALLPADQCPKITYPKPDNKLTFDRLSSVFVS
                     NTNHAEDQPVHLTLKDASVPVSINLAKYAGPESRYCPAGVYEFVKNEDGSDRLQINAQ
                     NCVHCKTCDIKDPTQNIVWVTPEGGGGPNYAGM"
     gene            1848..2951
                     /locus_tag="VIN75_02705"
     CDS             1848..2951
                     /locus_tag="VIN75_02705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8973100.1"
                     /translation="MAPLGKLVDALLTRDRHQRLRLMQALFASLLMCCCVLALHIAAL
                     FGLVDDGPIWWWSGASVAGLLVVVTLIRSGINKKFADPAMTMQQMLYATLCAAWAYSF
                     AGVAHNVVGIILAVILMFGMFGLSSRQVIWLGFYTLGVFGVVMAWASQRNPDHYPPGE
                     EIVYFMMLCIMVVGVGLLTVQLHRMRDRLRRQKVELEAALAHIHRLATHDDLTGLVNR
                     RHMQELLENERMRLERSEQDWCVALIDLDHFKSVNDMHGHAIGDEVLRALSRHAHTLI
                     RRTDVLARWGGEEFVLLLPNTPIAMAMNSLERLREHFHAHPLVVQGIELPVSFSAGLT
                     EHQRGETVAQTLERADKLCYQAKTLGRNRVERG"
     gene            complement(3216..3671)
                     /locus_tag="VIN75_02710"
     CDS             complement(3216..3671)
                     /locus_tag="VIN75_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973101.1"
                     /translation="MARPRVVPGEAEWAGHEQDLEAQYAHRLYFGKTTDEIMYDFRDL
                     VIERASELREVPRPVFQYYVFAFVDLFASPGESAEQADCASVFLLMLCGRERHDPGSV
                     AEIYDDLRATVEHIANNQAFYDADVDIYGDFREHAAELAALCGRGDGRG"
     gene            complement(3671..4801)
                     /locus_tag="VIN75_02715"
     CDS             complement(3671..4801)
                     /locus_tag="VIN75_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY8973102.1"
                     /translation="MSGDAFDKPAAGPLRWLPQRLVQSQDAPPQEQARAPEGFDSDDS
                     APESAPTDGDAHSDEPTPPPPVRWLRVRHETSYRYDAEVEGALHLAHLRPRDTATQQV
                     RVWSLVVSPHPDPGVDLASGIRETIDPWGNARQVFAHSQVHDQLDVVSAFEAGVSAQS
                     LPAPGSSRPWRELARDLRLHAGARMHDADAGAAAELSTFEAAEFALSSALAPRAKALA
                     DWARPPAEAHAGIVELALALMHRVHAEFEYLPQSTSVTTGALEALSLRHGVCQDFAHV
                     LIGACRSLGLAARYVSGYLLTQPPPGQPRLVGADASHAWAAVWCPPHGWLALDPTNDV
                     VVGQDHVTLAWGRDYADVAPLRGVIRGGSGNAPRVAVTVEPL"
     gene            complement(4798..7419)
                     /locus_tag="VIN75_02720"
     CDS             complement(4798..7419)
                     /locus_tag="VIN75_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY8973103.1"
                     /translation="MSTSAQTSFLPDDAPEASALARAAALRATTGSFDELHGGTAEAA
                     SAGAALAPGWQAFFEAIGADGWVDLAQTRARIRARVQDDGVTYNVYAEGAAAASAWPL
                     EPLPFLIDAASWAAIERGVVQRARLLDAVLADVYGSRDLLDEGLLPASLVLAHPQYLR
                     AMHGCRPAGGVRLHVAAFDLARGSDGDWRVVSQRTQAPSGLGYLLQNRLIVSQQFPTA
                     FRDLHVQRIASGFLGLLQGLQRLSPEGDDARVVLLTPGPHNETYFEHAFLARYLGITL
                     VEGGDLTVRGERLFLKTLHGLEPVHVLLRRVDDEWLDPLELRGDSALGVPGLLGVIRA
                     EQVVVANAPGAGFLESPGLAAFWPAVARRLLGEELLLPSTTSWWCGEDAVWEAQRERL
                     ADFVVAPTFPASETTQGFAPHGVAELSPDARAALLAAIDARPAAYTLQSRVRPSEMPV
                     WNEDALEPRAALVRVYAMTDGRGGWRVMPGGLTRVATRRDAAQDAWLSMQHGSVSVDT
                     WVMTEGEVDPTTLLPRPLSPADLARSRRTVTSRSAENLYWLGRYTERADNSVRLARIT
                     LESLNNANPALLDALGRLALRNGLIGSGVPSPLQSVRVFERALVHALADPATTSIGYN
                     LRALRQCAQALRERLSVEHWKLIDGVGRDFEQRLAAVLDPRDSASSAGGTGHAVESVG
                     DVLGVLQRAATQLAAITGAQSDRMTRDDGWRLLSVGRQTERLDFLAQALMLGVQLDLP
                     AREDGFALLLNLFDSTITYRAQFQARRELPPLLHLLVLEPDNPRSLAGVAHTLRDRLT
                     RLARHESRWVQRVLDELPSPQDWSLDALCALDEAGGHAALFSALQGLSERNLALSAEI
                     GRRLFSHVGPGDHTVWQ"
     gene            complement(7514..>8749)
                     /locus_tag="VIN75_02725"
     CDS             complement(7514..>8749)
                     /locus_tag="VIN75_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY8973104.1"
                     /translation="RLSRLSSEKFLVDGKHTGTGGGNHFVLGGATPADSPFLRRPDVL
                     GSLLAYWHNHPSLSYLFSGLFIGPTSQAPRIDEARNDQLYELEIALAEIARETRERGE
                     AVPPWLVDRSLRNILVDATGNTHRSEFCIDKLYSPDSSTGRLGLLELRAFEMPPHARM
                     SVVQQLLLRALVARFWNEAYDQPLVRWGTELHDRFLLPHFVQADFADVLIEMAGAGMP
                     FDPEWFAPHLEFRFPKLGEFASAGVHVTLRSALEPWHVMGEEGSAGGTVRYVDSSLER
                     LEVKVTGLVDSRHVLTCNGQPVPLQPTGRVGEFVGGVRYRAWRPPSGLHPTIPVQAPL
                     TVDLVDLWSKRSLGGCRYHVAHPGGRNYETLPVNAYEAESRRLARFFALGHTPGTMNV
                     ARPRRSDEFPFTLDLRRNR"
BASE COUNT         1342 a         3068 c         3159 g         1180 t
ORIGIN      
        1 caccttcggc ggcggcttcc tgtaccacct ggaaggcaac aaggtcacgc tcggcttcgt
       61 ggtcggcctg gactacaaga acccgtggat gagcccgttc gaggagatgc agcgctggaa
      121 gacgcacccg gccatccgcg cgcacatcct gtccggcgcg gtgatggatc cgatcgcgat
      181 caacgagctg ctgccgaact ggaaggccga cggcggcccg ctgctgcagc cggtgaccgg
      241 cgacgagttc ctgttcctgt cggagacggg ttccaggagc gtgcccgagt ggctgatgcc
      301 cgcgccgttc cacaaccacg gcaactacgt catcagcctg ggcaacgtca ccaagtggat
      361 ggcgagccag gccgaggcgc tgggcgtcga gatcttcccc ggcttcgccg cggcggaggt
      421 gctgtacgac gacaagggcg cggtcaaggg cgtggccacc ggcaacgtcg gcgtgggccg
      481 cgacggcgag ccgcacgacg gcttccagct gggcatggaa ctgcacggca agtacacgat
      541 cttcgcggag ggcgcgcgcg gccacctcgg caagcaggtg ctcgccaagt acgacctgat
      601 ggcgggtcgc gacccccaga gctgggccat cggcatcaag gagctgtggg aggttcccgc
      661 cgacaaggcc aagcccggcc acgtcgtgca ctcggccggc tggccggtga ccgacgacac
      721 cttcgccggc ggcttcctgt accacctcga gggcaacaag gtcacgctcg gctacatcgt
      781 cggcctcgac tacaagaacc cgtggatgag cccgttcgag gagatgcagc gctggaagac
      841 gcatccggac atccgcaagc acatcgaggg cggcaagcgc ctctcgtacg gcgcccgcgc
      901 catcaacaac ggcggcctgc agtgcctgcc caagctggtg ttcccgggcg gcgcgctcgt
      961 gggctgcgac gccggcacgc tcaacgccgc ccgcatcaag ggcagccacg ccgccatcaa
     1021 gacgggcatg ctggccgccg aggccgcgtt cgccgcgctg caggccggcc gccagggcga
     1081 cgagctctcg gcctacccgg aagccttcga gaagagctgg ctgcacaccg agctgcagcg
     1141 caccaagaac ttcaagctgt ggttcaagaa gggcaagctg gtcggcaacc tgatgaccgg
     1201 catcgagcaa tggctgatgc ccaagctggg catgatcccg ccgtggacgc tgcatggcac
     1261 caggaaggac aacgaggcgc tgctgccggc cgaccagtgc ccgaagatca cgtatccgaa
     1321 gcccgacaac aagctcacct tcgaccgcct gagctcggtg ttcgtcagca acaccaacca
     1381 cgcggaagac cagccggtgc acctgacgct caaggacgcg tcggtgcccg tgagcatcaa
     1441 cctggccaag tacgcgggcc ccgagagccg ctactgcccg gccggcgtct acgagttcgt
     1501 caagaacgag gacggcagcg accgcctgca gatcaacgcg cagaactgcg tgcactgcaa
     1561 gacctgcgac atcaaggatc cgacgcagaa catcgtgtgg gtcacgcccg aaggcggcgg
     1621 ggggccgaac tacgcgggca tgtgaacctg ccggccagcc gcctcgacgc ccgcctcgcg
     1681 cgggcgtttg tcattggtgc ccgcgccgct ggacgtccgt tcccgggcgc cttcgccgcg
     1741 cgagaaagct gcaactgcag gtgatattgg gcacctttcg ggtgcaacgc cggttcaatc
     1801 tccgttagtc ttcggtcaca aacgacaaaa cgggacaacg acagcacatg gcgcccctgg
     1861 gcaagctcgt cgacgcactc ctcacccggg accgccatca gcgcctgcgc ctgatgcagg
     1921 ccctgttcgc gtcgctgctg atgtgctgct gcgtgctcgc gctgcacatc gcggccctgt
     1981 tcgggctggt cgacgacggg ccgatctggt ggtggtcggg ggcgtcggtc gccgggctgc
     2041 tggtggtcgt cacgctgatc cgctcgggga tcaacaagaa gttcgccgac ccggcgatga
     2101 cgatgcagca gatgctgtac gccacgctgt gcgcggcctg ggcctactcg ttcgcgggcg
     2161 tcgcgcacaa cgtggtgggc atcatcctgg cggtgatcct gatgttcggc atgttcgggc
     2221 tgtcgagccg ccaggtgatc tggctgggct tctacacgct gggcgtgttc ggcgtggtga
     2281 tggcatgggc gtcgcaacgc aaccccgacc actatccgcc gggcgaggag atcgtctact
     2341 tcatgatgct ctgcatcatg gtggtgggcg tggggctgct caccgtgcag ctgcaccgca
     2401 tgcgcgaccg gctccggcgg cagaaggtgg agctggaggc cgcgctggcg cacatccacc
     2461 ggctggccac gcacgacgac ctcaccgggc tggtcaatcg ccggcacatg caggagctgc
     2521 tcgagaacga gcgcatgcgg ctcgagcgca gcgagcagga ctggtgcgtg gcgctgatcg
     2581 acctggacca cttcaagtcc gtcaacgaca tgcacggcca tgccatcggc gacgaggtgc
     2641 tgcgcgcgct gagccgccac gcccacacgc tgatccgccg caccgacgtg ctggcgcgct
     2701 ggggcggcga ggagttcgtg ctgctgctgc ccaacacgcc gatcgcgatg gcgatgaact
     2761 cgctggagcg cctgcgcgag cacttccacg cgcacccgct ggtcgtgcag ggcatcgagc
     2821 tgccggtctc gttctccgca gggctgaccg agcaccagag gggcgagacg gtggcgcaga
     2881 cgctggagcg ggcggacaag ctgtgctatc aggccaagac gttggggcgc aatcgggtcg
     2941 agcggggctg agcaaacctg gcggcgcatc gggccgccaa catcttgcga tggcgcgccg
     3001 aggtgccgcc gaaggggtct ggcacctcct cgaacgcgca ggcatgcacg ccttcagctg
     3061 cgatgccgat cgcgtgcgag gccgtgcctg acgccgatcg ccggggatcg gcgtcaagca
     3121 tctttgccct tcgcgggacg cgcggtggct tcggccagct tggcagtgcg aaggacgggc
     3181 atgccagaca ccacgcgtat taggcagcca aggtatcagc ctcgcccatc accccggccg
     3241 cagagcgccg ccaactcggc agcgtgctca cggaaatcgc cgtaaatatc gacatccgcg
     3301 tcgtagaacg cctggttgtt ggcgatgtgt tcgacagtcg cacgcaggtc atcgtagatc
     3361 tctgcaacgc tgccggggtc gtgcctctcg cgaccgcaca gcattagcag gaagacgctc
     3421 gcgcagtcgg cttgctcggc cgattcgccc ggcgacgcga acaagtcgac aaatgcgaag
     3481 acgtagtact ggaagacagg acgtggcacc tctcgcagct cgctcgcgcg ctcgatgacc
     3541 aagtccctga agtcgtacat gatctcgtcg gtggtcttgc cgaaatagag gcgatgggcg
     3601 tattgagcct cgaggtcttg ctcgtgcccc gcccattccg cctcgccagg aaccactcgc
     3661 ggtcgcgcca tcacaacggc tccaccgtca ccgccacccg cggcgcgttg cccgagccgc
     3721 cgcggatcac gccgcgcagc ggggccacgt ccgcgtagtc gcggccccag gcgagcgtca
     3781 cgtgatcctg gccgacgacg acatcgttgg tcgggtcgag cgccagccag ccgtgcggcg
     3841 gacaccagac ggcggcccag gcatgcgagg cgtcggcgcc caccaggcgg ggctggccgg
     3901 gcgggggctg ggtcagcagg tagccgctga cgtagcgggc ggccaggccc agcgagcggc
     3961 aggcgccgat cagcacgtgg gcgaagtcct ggcagacacc gtggcgcagc gacagcgcct
     4021 ccagcgcgcc cgtggtcacg gaggtcgact gcggcaggta ctcgaactcg gcatgaaccc
     4081 ggtgcatcag cgcgagcgcg agttcgacga tgcccgcgtg cgcctcggcc gggggccgcg
     4141 cccagtcggc cagcgccttg gcgcgcggcg ccagcgcgga cgacagcgcg aactcggccg
     4201 cctcgaacgt gctgagctcg gccgcggcgc ccgcgtccgc gtcgtgcatg cgcgcgccgg
     4261 cgtgcaggcg caggtcgcgc gcgagctcgc gccacggccg gctgctgccc ggcgcgggca
     4321 gcgactgcgc gctcacgccg gcctcgaacg cggagacgac gtccagctgg tcgtgcacct
     4381 gcgagtgcgc gaacacctgg cgcgcgttgc cccacggatc gatcgtctcg cggatgccgc
     4441 tggccaggtc gactcccggg tccggatgcg gcgagacgac cagcgaccac acgcgcacct
     4501 gctgcgtggc ggtgtcgcgc gggcgcaggt gggccaggtg cagcgcgccc tccacctcgg
     4561 cgtcgtagcg gtagctggtc tcgtggcgca cgcgcagcca gcgtacgggg ggcggaggcg
     4621 tcggctcgtc gctgtgcgcg tcgccgtcgg tcggcgcgga ctccggcgcg ctgtcgtcgc
     4681 tgtcgaagcc ctccggcgca cgcgcctgct cctgcggcgg cgcatcctgc gactgcacga
     4741 ggcgctgcgg cagccagcgc aaagggcctg ccgcgggctt gtcgaaggcg tcgccactca
     4801 ttgccacacc gtgtggtcgc ccggtccgac gtgcgagaac aggcggcgcc cgatctcggc
     4861 cgacagcgcg aggttgcgct cgctcaagcc ttgcagcgcg gaaaacagcg ccgcatggcc
     4921 gcccgcctcg tccagcgcgc acagcgcgtc cagcgaccag tcctgcgggg acggcagctc
     4981 gtcgagcacg cgctgcaccc agcgcgactc gtggcgcgcc aggcgcgtga gccggtcgcg
     5041 cagcgtgtgc gcgacgccgg ccagcgagcg cgggttgtcg ggctcgagca ccagcaggtg
     5101 cagcagcggc ggcagctcgc ggcgggcctg gaactgcgcg cggtaggtga tggtggagtc
     5161 gaacaggttc agcagcagcg cgaaaccgtc ctcgcgcgcg ggcaggtcga gctgcacgcc
     5221 cagcatcagc gcctgcgcga ggaaatcgag ccgctcggtc tggcggccga cgctcagcag
     5281 gcgccagccg tcgtcgcgcg tcatgcggtc ggactgcgcg ccggtgatgg cggccagctg
     5341 cgtcgcggca cgctgcagca cgcccagcac gtcgcccacc gactcgaccg cgtggccggt
     5401 gccgcccgcg gaggacgccg agtcgcgcgg gtcgagcacc gcggccaggc gctgctcgaa
     5461 gtcgcggccc accccgtcga tgagcttcca gtgctccacc gacagccgct cgcgcaacgc
     5521 ctgcgcgcat tgccgcagcg cgcgcaggtt gtacccgatg ctggtggtgg ccggatcggc
     5581 cagcgcatgc acgagcgcgc gctcgaacac gcgcaccgac tgcagcggcg acggcacgcc
     5641 gctcccgatg agcccgttgc gcagcgcgag gcgccccagc gcgtccagca gcgccgggtt
     5701 ggcgttgttc agcgattcca gcgtgatgcg cgccagccgc acgctgttgt cggcgcgctc
     5761 ggtgtagcgg cccagccagt acaggttctc ggccgaccgg ctggtgacgg tgcggcgcga
     5821 gcgcgcgagg tcggccgggc tcagcggccg gggcagcagc gtggtggggt cgacctcgcc
     5881 ctcggtcatg acccaggtgt ccacgctgac gctgccgtgc tgcatcgaca gccaggcatc
     5941 ctgcgcggcg tcgcggcgcg tggccacgcg cgtgagcccg cccggcatca cgcgccagcc
     6001 gccacggccg tcggtcatcg cgtagacgcg caccagcgcc gcgcgcggct ccagcgcgtc
     6061 ctcgttccac acgggcatct cggacggccg cacgcgcgac tgcagcgtgt acgcggcggg
     6121 acgcgcgtcg atggccgcca gcagcgcggc gcgcgcgtcg ggcgacagct ccgcgacgcc
     6181 gtgcggcgcg aagccctggg tggtctcgct ggcggggaac gtgggcgcga cgacgaagtc
     6241 ggcgaggcgc tcgcgctgcg cctcccagac ggcgtcctcg ccgcaccacc agctggtggt
     6301 cgacggcagc agcagttcct cgccgagcag ccggcgcgcg accgcgggcc agaacgcggc
     6361 caggcccggg ctctcgagga agcccgcgcc cggcgcgttg gccaccacca cctgctccgc
     6421 gcggatcacg cccagcaggc ccggcacgcc gagcgcggag tcgccgcgca gctccagcgg
     6481 gtcgagccac tcgtcgtcga cgcgccgcag cagcacgtgc acgggctcca ggccgtgcag
     6541 cgtcttgagg aacagccgct cgccgcgcac ggtgaggtcg ccgccctcga ccagcgtgat
     6601 gcccaggtag cgggccagga acgcgtgctc gaaataggtc tcgttgtgcg ggcccggcgt
     6661 cagcagcacg acgcgcgcgt cgtcgccctc ggggctcagg cgctgcaggc cctgcagcag
     6721 gcccaggaag cccgacgcga tgcgctgcac gtgcaggtcg cggaaggcgg tggggaactg
     6781 ctgcgagacg atcagccggt tctgcagcag gtagcccagc cccgacggcg cctgcgtgcg
     6841 ctggctgacg acgcgccagt cgccgtcgga cccgcgcgcg aggtcgaacg cggccacgtg
     6901 caggcgcacg ccgcccgcgg gccggcagcc gtgcatggcg cgcaggtact gcggatgggc
     6961 gaggacgagc gaggcgggca gcaggccctc gtcgagcagg tcgcggctgc cgtagacgtc
     7021 ggccagcacc gcgtcgagca ggcgcgcgcg ctgcacgacg ccgcgctcga tggcggccca
     7081 ggacgccgcg tcgatgagga acggcagcgg ctccagcggc cacgcgctgg cggccgcggc
     7141 gccttcggcg tagacgttgt aggtgacgcc gtcgtcctgc acgcgagcgc ggatgcgtgc
     7201 ccgcgtctgc gcgaggtcga cccagccgtc ggcgccgatg gcctcgaaga aggcctgcca
     7261 cccgggcgcc agcgcggcgc cggccgacgc ggcctcggcc gtgccgccgt gcagctcgtc
     7321 gaagctgccg gtcgtggcgc gcagcgccgc ggcgcgggcc agcgcggagg cttcgggcgc
     7381 gtcgtcgggg aggaagctgg tctgggcgga ggtcgacatc tattcagtgg cgtcgctgat
     7441 ctcggcgggc gagggtcgca cccgagccca accgagctcc ccgtcgggga gcagcgacgg
     7501 gaggagcgag gggtcaacga tttctgcgca agtccagcgt gaatgggaat tcgtcgctgc
     7561 gccgcggtcg cgcgacgttc attgtcccag gtgtgtgccc cagcgcgaag aagcgcgcga
     7621 ggcgccggct ctcggcctcg tacgcgttga ccggcagcgt ctcgtagttg cggccgcccg
     7681 gatgcgcgac gtggtagcgg cagccgccca gcgagcgctt gctccacagg tcgacgagat
     7741 cgaccgtgag cggcgcctgc acggggatcg tcggatgcag gcccgacggc ggacgccagg
     7801 cgcggtagcg cacgccgccg acgaactccc cgacgcgccc cgtcggctgc aagggcaccg
     7861 gctggccgtt gcacgtcagc acgtggcgcg agtcgacgag gccggtgacc ttgacctcca
     7921 gccgctccag cgacgagtcg acgtagcgca cggtgccgcc cgcgctgccc tcctcgccca
     7981 tcacgtgcca gggctccagc gcgctgcgca gcgtgacgtg cacgcccgcc gacgcgaact
     8041 cgccgagctt ggggaagcgg aactccaggt gcggcgcgaa ccactcgggg tcgaacggca
     8101 tgccggcgcc ggccatctcg atcagcacgt cggcgaagtc ggcctgcacg aagtgcggca
     8161 gcaggaagcg gtcatgcagc tcggtgcccc aacgcaccag tggctggtcg taggcctcgt
     8221 tccagaaccg cgcgaccagc gcccgcagca gcagctgctg cacgacgctc atgcgcgcgt
     8281 gcggcggcat ctcgaaggcg cgcagctcga gcaggcccag gcgcccggtg gacgaatcgg
     8341 gcgagtacag cttgtcgatg cagaactcgc tgcggtgcgt gttgccggtg gcgtcgacga
     8401 ggatgttgcg caggctgcgg tcgaccagcc acggcggcac cgcctctccc cgctcgcggg
     8461 tctcgcgggc gatctcggcc agtgcgatct ccagttcgta cagctggtcg ttgcgcgcct
     8521 cgtcgatgcg cggcgcctgg ctggtcgggc cgatgaacag gcccgagaac aggtagctca
     8581 gcgacggatg gttgtgccag taggcgagca ggctgcccag cacgtcgggc cggcgcagga
     8641 acggcgagtc ggccggcgtg gcgccgccca gcacgaagtg gttgccgccg ccggtgcccg
     8701 tgtgcttgcc gtcgaccagg aacttctccg acgacagccg cgacagccg
//
LOCUS       DATCRO010000101         5685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1103783, whole genome shotgun sequence.
ACCESSION   DATCRO010000101 DATCRO010000000
VERSION     DATCRO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103783"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="VIN75_02730"
     CDS             <1..1804
                     /locus_tag="VIN75_02730"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973105.1"
                     /translation="LATFLLLRAEPQARRSHRLLAAGYLYAGLMALMHVLVYPGALLP
                     GRPVFGDHETVTWLFNAWHAGFPLWVIAAAVAELRGRSRRRRRSPELLAILVFAAVVA
                     ACLLPASSFAPGSFATVFTQPGAIISFACAGLSLAGLALLLATGLTRRLLWLWMSLVL
                     AADAAGMVASTAGGGRYTLAWYGTRVEGVLASVVVLVLLTTHFRAVQATLVATVDDLR
                     RRTDALQAEIGRRERAERMLVQAQKLEAVGQLAAGLAHDFNNLMQVIAARLEILRRRA
                     GTNVDEDVAVVRRSIRKAEGLTRQLMLFTGRRQAQPQPVRLQRLLPDLVGMLLPLVRS
                     DIALSLDAPADVPPVHVDVEEFEAALTNLVANARDAMPRGGAIQVSLAAPPAATGEPA
                     WVELRVRDAGTGMPAEVVERVFEPFFTTKEPGKGTGLGLSQVQAFARSSGGSAAIDSA
                     PGQGTIVTLRLPALAADADVAPADGAEATAPRLVRGRSILLVDDSDDVRESTALLLEH
                     AGHVVRCAANAGEALALLSGGYAPDALVSDIVMPGELDGFALAREVARRWPAMRIVLA
                     TGYSVAADQAREDGLTVLQKPYASRDLMRALEAA"
     gene            complement(1913..2242)
                     /locus_tag="VIN75_02735"
     CDS             complement(1913..2242)
                     /locus_tag="VIN75_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY8973106.1"
                     /translation="MPHAGLMRLGRAALACAFAALLAPAPGAAKPPPAATASSAAASA
                     SPAAPRLVEDGHYRNVDGVVVHAPAHTDTGATPAGASAQCRDGSFSFSAHRRGTCSHH
                     GGVARWL"
     gene            2629..4920
                     /locus_tag="VIN75_02740"
     CDS             2629..4920
                     /locus_tag="VIN75_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973107.1"
                     /translation="MLMAIGLVVVVAWTLAAVDPASAWAWKLDSFHWTCAFCAAAWIV
                     RRAAAVPDPALRRVRQFFALGLALLAAGQLAWDALQFAGWNPFPGIPNLLFLLLSPCF
                     VLGFAAMADDAVPRSTWTSALLDVAGCALAVLAFTLALYLPHAAQQTTLQFATLTAYP
                     VLLFTAAATGLVVQLHLRQRPTGATLALVGGLAGYGALWLAWDLAFLDGRMQAGSPMG
                     LVFSLFPLLLAWGAARWQPARDAGARHDRLCEALLRQIPLAMVAFTSAAAGFIALGDR
                     VPLEARPPLRVLVVLVIVCAVVRQTQQLRDRDSLLAAERAVAEGQAKLQHLAHHDPLT
                     GLPNRMLLRDRVTQALATASRRGEKVALMFIDLDRFKEVNDTLGHAVGDALLCHVAAE
                     FSQLLREEDTVCRQGGDEFAIVLTDIDSPANAAQAVERVMTLSNRRATIAGHDLPMSM
                     SVGIAMFPQDGEDFDGLMQCADTAMYRAKASGRNAYRFYDPQMNVEASERSRLRWLLG
                     HAIERDELHLVYQPYVNLRSGELAGAEALLRWTSAELGPVGPDQFIPVAEDGGQIVEI
                     GAWVLRQACAQAAQWLREGLAVPSVAVNLSILQFRHGDLVQQVAGALRDSGLPPQHLE
                     LEITESVLMNDTDRVMATADSLKALGVRLAIDDFGTGYSSLAYLQRLSVDKLKIDQSF
                     VRSASTGKGSTAIVRAIVDMARALDLAVVAEGVETPAERDFLVATQCTLGQGWLFARP
                     MTPDRFEAWMQERATEAAASVAG"
     gene            5021..5344
                     /locus_tag="VIN75_02745"
     CDS             5021..5344
                     /locus_tag="VIN75_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973108.1"
                     /translation="MYDQLDQMPAIEEIEDRPGQLRPRGMPDAVFYACCCALALLPAS
                     LAAVLLHNLIGDPFVFLAAVVVSVWVVTTVALVKIAGPLLRCRAIVAAGGLVSVAMCS
                     LFLID"
     gene            complement(5354..>5685)
                     /locus_tag="VIN75_02750"
     CDS             complement(5354..>5685)
                     /locus_tag="VIN75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973109.1"
                     /translation="NSTCETNERVRTLGYRIVGWHVDSCDWGYDGPSGLTAAEALSCG
                     VLAPYRHDFVGHVVASVRAHRGGIVLMHETQHISVDNLDTLITKLETEGYRFSTPVAP
                     EFATSLR"
BASE COUNT          693 a         2103 c         2082 g          807 t
ORIGIN      
        1 cctcgcgacc ttcctgctgc tgcgcgccga gccgcaggcg cggcggtcgc accggctgct
       61 ggccgcgggc tacctgtacg ccgggctgat ggccctgatg cacgtgctcg tctaccccgg
      121 cgcgctgctg cccggccgcc cggtgttcgg cgaccacgag accgtcacct ggctgttcaa
      181 cgcgtggcac gccggcttcc cgctgtgggt gatcgccgcg gccgtcgccg agctgcgcgg
      241 acgctcgcgg cgccggcgcc gctcgccgga actgctcgcg atcctcgtgt tcgccgccgt
      301 ggtcgcggcc tgcctgctgc cggcgtcgtc gttcgcgccc ggctcgttcg ccaccgtctt
      361 cacccagccc ggcgcgatca tcagcttcgc ctgcgcgggc ctgtcgctgg ccggcctcgc
      421 gctgctgctg gcgaccggcc tcacgcgccg cctgctgtgg ctgtggatgt cgctggtgct
      481 ggcggccgac gccgccggca tggtggccag caccgccggc ggcggacgct acacgctggc
      541 ctggtacggc acgcgcgtcg agggcgtgct ggcgtcggtg gtcgtcctgg tgctgctgac
      601 gacgcacttc cgcgccgtcc aggccacgct ggtcgccacc gtcgacgacc tgcgccggcg
      661 caccgacgcg ctgcaggccg agatcggccg ccgcgagcgc gccgagcgga tgctggtgca
      721 ggcgcagaag ctcgaggccg tcggccagct ggccgcgggc ctggcgcacg acttcaacaa
      781 cctgatgcag gtgatcgccg cgcggctgga gatcctgcgc cggcgcgcgg gcaccaacgt
      841 cgacgaggac gtcgcggtcg tgcggcgcag catccgcaag gccgagggcc tgacacgcca
      901 gctgatgctg ttcaccggcc gccgccaggc gcagccgcaa ccagtgcgcc tgcagcgcct
      961 gctgcccgac ctggtgggca tgctgctgcc gctggtgcgg tcggacatcg cgctgtcgct
     1021 cgacgcgccc gcggacgtgc cgccggtgca cgtcgacgtc gaggagttcg aggccgcgct
     1081 gacgaacctg gtcgccaacg cgcgcgacgc gatgccgcgc ggcggcgcca tccaggtcag
     1141 cctcgccgcc ccgcctgcgg cgacgggcga accggcatgg gtggaactgc gcgtgcgcga
     1201 cgcgggcacc ggcatgccgg ccgaggtggt cgaacgcgtg ttcgagcctt tcttcacgac
     1261 caaggaaccg ggcaagggca ccggcctggg cctgagccag gtgcaggcgt tcgcgcgcag
     1321 cagcggcggc agcgccgcca tcgacagcgc gcccggccag ggcaccatcg tgacgctgcg
     1381 cctgcccgcg ctcgccgcgg acgcggacgt cgcgcccgcg gacggcgccg aggccaccgc
     1441 gccgcgcctg gtgcgcggcc gctccatcct gctggtcgat gacagcgacg acgtgcgcga
     1501 gtccaccgcg ctgctgctgg agcacgcggg ccacgtggtc aggtgcgccg ccaacgccgg
     1561 cgaggcgctg gcgctgctgt cgggcggcta tgcgcccgat gcgctggtgt ccgacatcgt
     1621 gatgcccggc gagctggacg gcttcgcgct ggcacgcgag gtcgcgcgcc gctggccggc
     1681 gatgcgcatc gtgctggcca cgggctacag cgtggccgcc gaccaggccc gcgaggacgg
     1741 cctgaccgtc ctgcagaagc cctacgccag ccgcgacctg atgcgcgcgc tcgaggccgc
     1801 ctgacctgca ccgtcagccg cgccggaccc gcttgcaggt ctcgccgtcg cgccagcgca
     1861 cccgcgcacg cagcctcgcc tcgccgcccg gacggccctc ccgcggcagc gcctacaacc
     1921 agcgcgccac gccgccgtga tgcgagcagg tgccgcgccg atgcgcgctg aagctgaagc
     1981 tgccgtcgcg gcactgcgcc gaggcgccgg cgggcgtcgc gccggtgtcg gtgtgcgccg
     2041 gcgcatgcac cacgacgccg tcgacgttgc ggtagtggcc gtcctcgacc agccgtggcg
     2101 cggccgggga ggcgctggca gcggcggatg acgcggtcgc ggcaggaggc ggcttcgcgg
     2161 cgccaggcgc gggcgccagc agcgccgcga acgcgcacgc caacgcggcg cggccaaggc
     2221 gcatcaggcc agcgtgcggc atgggcggag gcaggagcag tcgttcggcg gcatgcgttc
     2281 gcggcgcggg agttggcggt ccacgccagc gtagtcgtgc gcgcgggcga cggaggcacc
     2341 gcgggcgcgg cgcgccgggt gtccacgtga cgaaattcac cgctttcgcg ccccgccccg
     2401 cccgggcgca ggcacgatgc ctgttcacgc gtcgcgcctg ccgggctcgt cgtccgaccc
     2461 acgggctggc gtgagacact tcccatcagg ccgggcccgc cgcggttcgg cgcttcggcc
     2521 gtccggcggc cacgaagaca acgagcaggc cacgaggatc gagggcagat gccatccgac
     2581 agcgccaggg agcgcagcgt cacaccccgc cagccagggt cgccgaagat gttgatggcg
     2641 atcggcctgg tcgtggtcgt cgcctggacg ctcgcggcgg tggatcccgc ctccgcctgg
     2701 gcatggaagc tcgacagctt ccactggacc tgcgcgttct gcgccgcggc ctggatcgta
     2761 cgccgcgccg ccgccgtccc ggatcccgcg ctgcgccgcg tccggcagtt cttcgccctc
     2821 ggactggcgc tgctggccgc gggccagctc gcctgggacg cgctgcagtt cgcgggctgg
     2881 aacccgttcc ccggcatccc caacctgctc ttcctgctgc tgagcccgtg cttcgtcctc
     2941 ggcttcgccg cgatggcgga cgacgcggtg ccgcgttcga cgtggacgtc ggcgctgctg
     3001 gacgtggccg gctgcgcgct ggccgtgctc gccttcacgc tggcgctgta cctgccgcac
     3061 gccgcgcagc agaccacgct gcagttcgcg acgctgaccg cctaccccgt gctgctgttc
     3121 acggccgcgg ccaccggcct ggtcgtgcaa ctgcacctgc gccagcgccc gaccggcgcg
     3181 acgctggcgc tggtgggcgg actggccggc tacggcgcgc tgtggctggc ctgggatctc
     3241 gcgttcctcg acgggcggat gcaggccggc tcgccgatgg gcctggtctt ctcgctgttc
     3301 ccgctgctgc tggcctgggg cgccgcgcgc tggcagcccg cgcgcgatgc cggcgcgcgc
     3361 cacgaccgcc tgtgcgaggc gctgctgcgc cagatcccgc tcgcgatggt cgcgttcacg
     3421 tcggccgccg cgggcttcat cgcgctgggc gaccgcgtgc cgctggaagc gcgcccgccg
     3481 ctgcgcgtgc tggtggtgct cgtgatcgtg tgcgccgtcg tgcgccagac gcagcagctg
     3541 cgcgaccgcg acagcctgct cgccgcggaa cgcgccgtcg ccgagggcca ggccaagctg
     3601 cagcacctgg cgcaccacga cccgctgacc ggcctgccca atcgcatgct gctgcgcgat
     3661 cgcgtgacgc aggcgctggc cacggcgtcg cggcgcggcg agaaggtcgc gctgatgttc
     3721 atcgacctgg accgcttcaa ggaggtcaac gacacgctgg gccacgccgt cggcgacgcg
     3781 ctgctgtgcc acgtcgccgc ggagttctcg cagctgctgc gcgaggagga caccgtctgc
     3841 cgccagggcg gcgacgagtt cgcgatcgtg ctgaccgaca tcgactcgcc cgcgaacgcc
     3901 gcgcaggcgg tcgaacgcgt gatgacgctg tcgaaccggc gcgcgacgat cgcggggcac
     3961 gacctgccga tgtcgatgtc ggtgggcatc gcgatgttcc cgcaggacgg cgaggacttc
     4021 gacggcctga tgcagtgcgc cgacaccgcg atgtaccgcg ccaaggcctc gggccgcaac
     4081 gcctaccgct tctacgaccc gcagatgaac gtcgaggcca gcgaacgttc gcgcctgcgc
     4141 tggctgctgg gccacgcgat cgagcgcgac gagctgcacc tggtctacca gccctacgtc
     4201 aacctgcgca gcggcgaact cgccggcgcc gaggcgctgc tgcgctggac gtcggccgag
     4261 ctcggcccgg tcggtcccga ccagttcatc ccggtcgccg aggatggcgg gcagatcgtc
     4321 gagatcggcg cctgggtgct gcgccaggcc tgcgcgcagg ccgcgcagtg gctgcgcgag
     4381 gggctggccg tgccgagcgt ggcggtcaac ctgtcgatcc tgcagttccg ccatggcgac
     4441 ctggtgcagc aggtggccgg ggcgctgcgc gactccggcc tgccgccgca gcacctcgaa
     4501 ctcgagatca ccgagtcggt gctgatgaac gacaccgacc gcgtgatggc caccgccgac
     4561 agcctcaagg cgctgggcgt gcgcctggcc atcgacgact tcggcaccgg ctactcgagc
     4621 ctggcctacc tgcagcggct cagcgtggac aagctcaaga tcgaccagtc gttcgtgcgc
     4681 agcgcgtcca ccggcaaggg atcgacggcc atcgtgcgcg ccatcgtcga catggcgcgc
     4741 gcgttggacc tggcggtggt ggccgagggc gtcgagacgc ctgccgaacg cgatttcctg
     4801 gtcgccaccc agtgcaccct gggccagggc tggctgttcg ccaggccgat gacccccgac
     4861 cgcttcgagg cctggatgca ggaacgcgcc acggaggcgg cggcgagcgt cgccggttga
     4921 cgccacgcgg gcccgcgagg gcttgccatt tgcccgtatt gacgacaagg cgcccgcggc
     4981 gcgtaatcgg caggcgcacg ctcccttcag gaggccagcc atgtacgacc agctcgacca
     5041 gatgcccgcc atcgaggaga tcgaggatcg ccccgggcag ctgcgtccgc gcggcatgcc
     5101 cgacgcggtg ttctacgcct gctgctgcgc cctggcgctg ctgcccgcgt cgctggcggc
     5161 ggtgctgctg cacaacctga tcggcgaccc gttcgtgttc ctcgcggcgg tggtcgtgtc
     5221 ggtgtgggtc gtcacgacgg tggcgctcgt caagatcgcc gggccgctgc tgcgctgccg
     5281 cgcgatcgtg gccgccggcg ggctcgtgtc ggtcgcgatg tgctcgctgt tcctgatcga
     5341 ctgacgcgct gcgtcaacgc agcgacgtcg cgaactccgg cgccaccggc gtcgagaaac
     5401 ggtagccctc ggtttcgagc ttcgtgatca gcgtgtccag gttgtcgacg ctgatgtgct
     5461 gcgtctcgtg catcagcacg atgccgccgc ggtgcgcgcg cacgctggcc accacgtggc
     5521 cgacgaagtc atggcggtac ggcgccagca cgccgcagga caaggcctcg gcggcggtca
     5581 ggccggacgg gccgtcgtag ccccagtcgc acgaatcgac gtgccagccc acgatgcggt
     5641 agcccagggt gcgcacgcgc tcgttggtct cgcaggtgga attgc
//
LOCUS       DATCRO010000102         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_102333, whole genome shotgun sequence.
ACCESSION   DATCRO010000102 DATCRO010000000
VERSION     DATCRO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102333"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VIN75_02755"
     CDS             <1..668
                     /locus_tag="VIN75_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY8973110.1"
                     /translation="MGVSKATVSRVLRRAGLSRLTDLRPVEPIQRYEHDAPGELLHID
                     IKKLGRFDAAGHRITGDRHRRSSKVGWDFVFVAVDDHSRIAFTQIYPNEGKDSAVSFL
                     CASVRYFSTLGVPVQRVITDNGNGFRSAAFGEACLERGIAQKFTRAFRPQTNGKAERF
                     IQSAMREWAYGRAYVNSDARCRALPAWNHFYNWHRRHHGIGCLPPMSRLPSSRKNLLT
                     LHS"
     gene            complement(1047..1700)
                     /gene="alkB"
                     /locus_tag="VIN75_02760"
     CDS             complement(1047..1700)
                     /gene="alkB"
                     /locus_tag="VIN75_02760"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEY8973111.1"
                     /translation="MTLDLFAADAPPLEDEALAPGAVVLRGFAGAREAALKDGLAGVT
                     AASPFRHLVTPGGFRMSVAMTNCGELGWVSDRRGYRYDPVDPETGAPWPAMPEAFCAL
                     AGDAAARAGYPDFAPDACLVNRYEPGARLSMHQDRDEKDLRAPIVSVSLGLPAIFLWG
                     GDARGDKARRVALQHGDVVVWGGPSRLRFHGVLALKEGEHPFAGALRINLTFRKVRA"
     gene            complement(1697..2470)
                     /gene="xth"
                     /locus_tag="VIN75_02765"
     CDS             complement(1697..2470)
                     /gene="xth"
                     /locus_tag="VIN75_02765"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8973112.1"
                     /translation="MKIATYNVNGINGRLPRLLEWLDETRPDVACLQEIKTSDDTFPL
                     EAIHDAGYGAIWAGQKSYNGVAILARDATPVERLRHLPGAEDDTHSRYIEAEIDGVVV
                     ASLYLPNGNPQPGPKFDYKLAWFERFDRHAKSLLAEKRPVVLAGDYNVVPTDLDIYDT
                     RSWLDDALLQPESRAAYAKVLEQGWIDAIRAKHPDARIYTFWDYFRNRWARNAGLRID
                     HLLLSPDLADHLVDAGVDREQRGKEKASDHAPAWVELGR"
     gene            complement(2506..3186)
                     /locus_tag="VIN75_02770"
     CDS             complement(2506..3186)
                     /locus_tag="VIN75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8973113.1"
                     /translation="MPASAANTVSARPYGGLAMAERVAARRARFIEAGIELFGTQGFR
                     GATVRGICAAAGLTDRYFYESFASLEALLADVYRTVKDGFARRLTEESFGAEGWHGDD
                     AAVERQVTAAYEIWFDTVRDPRFARILLVEVLGVSPEMDALYEASARAMAELTITPLA
                     ATRPALKLSRQRRELLGRALVGAGLQVAKMWMTGGYKLPRRDVVRTCVLVATGTLAAL
                     RAEAASDA"
     gene            3289..4143
                     /locus_tag="VIN75_02775"
     CDS             3289..4143
                     /locus_tag="VIN75_02775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973114.1"
                     /translation="MAPPTPWTLRGGVAVLTGAASGIGAALAVDLARRGMQLALVDVN
                     AAGLQAVAAQARAAGSKVSTHALDVADHAAVAALPAAVLAEHGRVTVLVNNAGVALGG
                     RFEQVAAEDFDWLMAINFGGTVRLTRAFLPVLAREPAAQLVNVSSIFGIIAPPGQTAY
                     VASKFAVRGFSESLRHELEMAGSPVGVTLVHPGGVRTAISTNARLARNLDAAEVAREQ
                     ASWRSLLVMDPAKAADIIATGIERRSKRVLVGNDAKGAAIIQRLFPVTYWKHVARDLA
                     RRIRRQPA"
     gene            4156..>6297
                     /locus_tag="VIN75_02780"
     CDS             4156..>6297
                     /locus_tag="VIN75_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEY8973115.1"
                     /translation="MASWQARAARYLIRKRVRPAFGDMRDLMQVRKVMGQAMPPPKGV
                     AYTRDTLGGIPGEWVEREGAAQIAASPRPTLMYVHGGGFVGCSPRTHRPITAAFALQG
                     FRVFAPDYRLAPEHPFPAPLDDVATAWRALRAKHDGDSAGQRLVLAGESAGGNLALAL
                     LLKLRDAGERLPDAAALFSPATDVTGQSPSIEGNADRDPMFHGPSLEHLGKAYLGAAG
                     DATNPLVSPIFGDLAGLPPLLFHVAAEEALRDDGTRFAAKARAAGVHVETTVWPVVPH
                     AWQLLKDLPEARRSIARAGEFLKTATPDAIEHLDAIIVGAGLSGIGAAVHLQNDFPGR
                     RFAILESREAIGGTWDLFRYPGVRSDSDMFTLGYAFKPWTDSKSLADGPAIRDYIVET
                     AVEHGLDRQVRFGHKVVRADWSSADACWTLEIEQASQRRSRIRCNFLLACSGYYRYSE
                     GHAPEFAGVDAYQGRFVHPQFWPSDLDHAGKRVVVIGSGATAVTLVPEMARTAQHVTM
                     LQRSPTYVLSLPGRDPLAERLRRWLPRMLAYRLTRTKNIGVAMLFFNLSRRWPAMVRR
                     SLIGLVRQALGPSHDVDTHFTPRYDPWDQRVCFVPDGDLFEAIKDKRAEVVTDHVDTF
                     TPTGIRLRSGRELPADIVVSATGLKLNVLGDVAFAVDGKDVDFSNTLGYKGMMFSGVP
                     NLIYTFGYTNASWTLKADLTAGYASRLFAYMD"
BASE COUNT          955 a         2276 c         2148 g          918 t
ORIGIN      
        1 acatgggcgt gtccaaagcg acggtcagtc gcgtgctcag gcgagctggc ttgtcgcggc
       61 tgacagacct gcggcctgtc gagccgattc aacgctatga gcacgatgcg cctggcgaac
      121 tgcttcacat tgacatcaag aagctcggcc ggttcgacgc agctggccac cgcatcactg
      181 gcgatcgaca ccgtcgaagt tcaaaagtcg gctgggactt cgtcttcgtg gccgtggatg
      241 accatagccg aatcgcattc acgcagatct acccgaacga gggcaaggac agcgcggtca
      301 gcttcctatg cgcgtcagtt cgctacttct ccacacttgg cgttccagtg cagcgcgtga
      361 ttactgacaa cggcaacggt ttccgctctg cggcgttcgg ggaggcttgc ctcgaacgcg
      421 gcattgccca gaagttcact cgtgccttcc ggccccaaac caatggcaag gccgagcgat
      481 tcatccagtc ggccatgcgc gagtgggcgt acgggcgcgc ctacgtcaac tccgatgctc
      541 gttgccgcgc gctgcccgcc tggaaccact tctacaactg gcaccggcgc catcacggca
      601 tcggctgctt gccgccaatg tccagactcc cttcatcacg caagaacctg ttgacactcc
      661 acagttagcc gcaggcgtaa cccgccatgc cggcagatcg caacgccggc gaatggcggg
      721 ttacgcctgc ggctaacccg ccctacgaaa tgccgcgaga catcgcggtg cccggcgccg
      781 tcgatcgcgc gctgtcaacc cctcgcttgc ccgagccgcc gcgcggtttt ccacatcgtc
      841 tggggaaaag cctgtggaaa acgacgagcg ggcggcgcgc ggcccgccaa atcatgcgat
      901 ttccgctgga ttgcacgcag acgaggcagt gtcacgaatt tgccatcatc gtcatgtcaa
      961 ggcgatgccg aacatgacga ttcgccccaa tggtgtgctg cacaaacgcg cggcgacgcg
     1021 ccgcgcctgc gggaaaacgc gcgggatcag gccctgacct tgcggaaggt aaggttgatc
     1081 cgcagcgcgc ccgcgaacgg gtgctcgcct tccttgagcg cgagcacgcc atggaagcgc
     1141 agccgcgacg gcccgcccca cacgacgacg tcgccgtgct gcagcgcgac acgccgcgcc
     1201 ttgtcgccgc gcgcgtcgcc gccccacagg aagatggcgg gcaagcccag cgagaccgac
     1261 acgatgggcg cgcgcaggtc cttctcgtcg cgatcctggt gcatcgacag ccgtgcgccc
     1321 ggctcgtagc gattgacgag acacgcatcc ggcgcgaagt ccggataacc ggcgcgtgcg
     1381 gcggcatcgc cggcgagcgc gcagaacgcc tccggcatcg cgggccaggg cgcgcccgtc
     1441 tcggggtcga ccgggtcgta gcgatagccg cggcggtccg acacccagcc cagttcgccg
     1501 cagttggtca tcgccacgga catgcggaac ccgcccggcg tgacgagatg acggaacggc
     1561 gacgcggccg tgacgcctgc caggccgtcc ttcaacgcgg cctcgcgcgc gccggcgaag
     1621 ccgcgcagga cgaccgcgcc gggagcgagc gcttcgtcct cgagcggggg cgcgtcggcg
     1681 gcgaacaggt ccagcgtcat cggcccagtt ccacccaggc gggcgcgtgg tcgctcgcct
     1741 tctccttgcc gcgctgctcg cgatcgacgc ccgcatcgac gaggtgatcg gccagatcgg
     1801 gcgacagcag caggtggtcg atgcgcaggc ccgcgttgcg cgcccagcga ttgcggaagt
     1861 agtcccagaa cgtgtagatc cgagcatccg gatgcttcgc gcggatcgcg tcgatccagc
     1921 cctgctcgag caccttcgcg taggccgcgc ggctctcggg ctgcagcagc gcgtcgtcca
     1981 gccaggagcg cgtgtcgtag atgtccaagt cggtgggcac gacgttgtag tcgccggcca
     2041 gcaccacggg acgcttctcg gccagcagcg acttcgcgtg cctgtcgaag cgctcgaacc
     2101 atgcgagctt gtagtcgaac ttggggccgg gctgcggatt gccgttgggc aggtacagcg
     2161 acgcgacgac gacgccgtcg atctcggcct cgatgtagcg gctgtgcgtg tcgtcctccg
     2221 cgccgggcag gtggcgcagg cgctcgacgg gcgtcgcgtc gcgcgcgagg atcgccacgc
     2281 cgttgtacga cttctgtccg gcccagatcg cgccgtagcc ggcgtcgtgg atcgcctcca
     2341 gcgggaaggt gtcgtcggag gtcttgatct cctgcaggca ggcgacgtcg ggccgcgtct
     2401 cgtccagcca ctcgagcagg cgcggcaggc ggccgttgat gccgttgacg ttgtaggtgg
     2461 cgatcttcat gggcgcgagg cgagcattcg acatgcccag caacgtcagg cgtcgctcgc
     2521 cgcttccgcg cgcagtgccg cgagcgtgcc ggtggccacc agcacgcagg tgcgcacgac
     2581 gtcgcgccgc ggcagcttgt agccgccggt catccacatc ttggccacct gcagtccggc
     2641 gcccacgagc gcgcggccga gcagttcgcg ccgctgcctg gacagcttca gcgccggccg
     2701 cgtggccgcc agcggcgtga tggtgagctc ggccatggcg cgcgcagagg cctcgtacag
     2761 cgcgtccatc tcggggctga cgcccagcac ctcgaccagc aggatgcgcg cgaagcgcgg
     2821 gtcgcgcacg gtgtcgaacc agatctcgta ggccgcggtc acctggcgtt cgacggccgc
     2881 gtcgtcgccg tgccagccct cggcgccgaa cgattcctcg gtcaggcgtc gcgcgaagcc
     2941 gtccttgacc gtgcgataca cgtcggcgag caaggcctcc agcgacgcga acgactcgta
     3001 gaaatagcgg tcggtcaggc cggccgccgc gcagatgccg cgcaccgtgg cgccgcgaaa
     3061 gccctgcgtg ccgaacagct cgatgccggc ctcgatgaag cgcgcgcggc gcgcggccac
     3121 gcgctccgcc atcgcgaggc cgccgtaagg gcgcgcggaa acggtgttgg cggcggaggc
     3181 gggcatcccc ctatttgaca cgaatccacc ccgagcctac gatcggctcg atatctgacg
     3241 acacgcgtcg tcactttggc gacaccacga gcgaggacga aagacgacat ggccccaccg
     3301 accccctgga cgctgcgcgg cggcgtcgcc gtgctcaccg gcgcggcgag cggcatcggc
     3361 gcggcgctgg ccgtcgatct cgcacggcgc ggcatgcagc tcgccctggt cgacgtcaac
     3421 gccgccggac tgcaagccgt cgccgcccag gcgcgcgccg ccggctccaa ggtgagcacg
     3481 cacgcgctcg acgtcgccga ccacgcggcc gtcgccgccc tgcccgccgc ggtgctcgcc
     3541 gaacacgggc gcgtcacggt gctggtcaac aacgcgggcg tcgcgctggg cggccgtttt
     3601 gaacaggtgg ccgccgagga cttcgactgg ttgatggcca tcaacttcgg cggcacggtg
     3661 cgcctcacgc gcgcgttcct gcccgtgctc gcccgcgagc cggcggcgca gctggtcaac
     3721 gtgtccagca tcttcggcat catcgcgccg ccggggcaga cggcctatgt cgcgtcgaag
     3781 ttcgccgtgc gcgggttctc ggagtcgctg cgccacgagc tcgagatggc ggggtcgccg
     3841 gtcggcgtca ccctcgtcca tcccggcggc gtgcgcaccg cgatctcgac gaacgcgcgc
     3901 ctggccagga acctggacgc cgccgaggtg gcgcgcgaac aggccagctg gcgcagcctg
     3961 ctggtgatgg atccggccaa ggcggccgac atcatcgcca ccggcatcga gcgccgcagc
     4021 aagcgcgtgc tggtgggcaa cgacgccaag ggcgccgcga tcatccagcg gctgttcccg
     4081 gtgacgtact ggaagcacgt ggcgcgcgac ctcgcgcgcc gcatcaggcg ccaaccggcc
     4141 tgagaggagc agacgatggc cagctggcag gcgcgcgccg cccgttacct catccgcaag
     4201 cgggtgcgtc ccgcgttcgg cgacatgcgc gacctgatgc aggtgcgcaa ggtcatgggc
     4261 caggcgatgc cgccgcccaa gggcgtcgcc tacacgcgcg acaccctcgg cggcatcccc
     4321 ggcgaatggg tcgagcgcga aggcgccgcg cagatcgcgg ccagcccgcg ccccacgctc
     4381 atgtacgtgc acggcggcgg cttcgtcggc tgctcgccgc gcacgcaccg gccgatcacc
     4441 gcggccttcg cgctgcaggg cttccgcgtc ttcgcgcccg actaccgcct cgcacccgag
     4501 catccgttcc cggcgccgct ggacgacgtc gccaccgcgt ggcgcgcgct gcgcgcgaag
     4561 cacgacggcg attccgcggg ccagcgcctc gtgctggccg gcgagagcgc gggcggcaac
     4621 ctcgcgctgg cgctgctgct gaagttgcgc gatgccggcg aacgcctgcc cgacgccgcc
     4681 gccctcttct cgcccgccac cgacgtcacc ggccagagcc cgtcgatcga aggcaacgcc
     4741 gaccgcgacc cgatgttcca cgggccgtcg ctggagcacc tgggcaaggc ctacctcggc
     4801 gccgccggcg atgcgacgaa cccgctcgtc tcgccgatct tcggcgacct cgcgggcctg
     4861 ccgccgctgc tgttccatgt cgccgcggaa gaagcgctgc gcgacgacgg cacgcgcttc
     4921 gcggccaagg cgcgcgccgc gggcgtgcac gtcgagacca ccgtgtggcc cgtcgtgccg
     4981 catgcgtggc agctgctgaa ggacctgccc gaggccaggc gctcgatcgc gcgcgccggc
     5041 gagttcctca agacggccac gcccgacgcg atcgagcacc tcgacgcgat catcgtcggc
     5101 gccgggctct cgggcatcgg cgcggcggtg cacctgcaga acgacttccc cggccgccgc
     5161 ttcgcgatcc tggagagccg cgaggccatc ggcggcacgt gggacctgtt ccgctatccc
     5221 ggcgtgcgct ccgactccga catgttcacg ctcggctacg cgttcaagcc gtggacggat
     5281 tcgaagtcgc tggccgacgg gccggccatc cgcgactaca tcgtcgagac ggccgtcgag
     5341 cacggcctgg atcgccaggt gcgcttcggc cacaaggtgg tgcgcgccga ctggtcgtcg
     5401 gccgacgcgt gctggacgct cgagatcgaa caggcaagcc agcgccgctc gcgcatccgc
     5461 tgcaacttcc tgctcgcgtg cagcggctac taccgctaca gcgagggcca cgcacccgag
     5521 ttcgcgggcg tcgacgcgta ccagggccgc ttcgtgcatc cgcagttctg gccgtccgac
     5581 ctcgatcacg ccggcaagcg cgtggtcgtc atcggcagcg gcgccacggc ggtgacgctg
     5641 gtgccggaga tggcgcgcac cgcgcagcac gtcacgatgc tgcagcgctc gccgacctac
     5701 gtgctgtcgc tgccgggccg cgacccgctc gccgagcgcc tgcgccgctg gctgccgcgc
     5761 atgctggcct accgcctcac gcgcaccaag aacatcggcg tggcgatgct gttcttcaac
     5821 ctgtcgcggc gctggccggc gatggttcgc aggagcctga tcggcctcgt gcgccaggcg
     5881 ctcggcccgt cgcacgacgt cgacacccac ttcacgccca ggtacgaccc gtgggaccag
     5941 cgcgtgtgct tcgtgcccga cggcgacctg ttcgaggcga tcaaggacaa gcgcgcggag
     6001 gtggtgaccg accacgtcga caccttcacg ccgaccggca tccgcctgcg gtcgggccgc
     6061 gagctgccgg ccgacatcgt cgtctcggcc accggcctga agctgaacgt gctgggggac
     6121 gtcgcgttcg cggtcgacgg caaggacgtc gatttctcga acacgctggg ctacaagggc
     6181 atgatgttca gcggcgtgcc caacctgatc tacacgttcg gctacacgaa cgcctcgtgg
     6241 acgctcaagg ccgacctgac ggcgggctac gcgtcgcgcc tgttcgccta catggac
//
LOCUS       DATCRO010000103         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1324, whole genome shotgun sequence.
ACCESSION   DATCRO010000103 DATCRO010000000
VERSION     DATCRO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..543
                     /locus_tag="VIN75_02785"
     CDS             334..543
                     /locus_tag="VIN75_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973116.1"
                     /translation="MTDPQRPRDQEPPRRATPLQPDKPGSGARRERSGEQPEPSASHG
                     GQAGETRKLRRQEDSAVDNAREGYD"
     gene            579..1025
                     /locus_tag="VIN75_02790"
     CDS             579..1025
                     /locus_tag="VIN75_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8973117.1"
                     /translation="MTQVCEVMTRDVRTLAPTETLMKAAQTMDELDVGVIPVCDGDRL
                     LGVVTDRDIVVRGVAAGCMVDQATLREVMSEPAHWCYDDDAVEDAARTMGEHQLRRVP
                     VIDHDRRLVGIVALADLATRGQAMSAGDALEAVSQGTAPASSPAAR"
     gene            1063..1608
                     /locus_tag="VIN75_02795"
     CDS             1063..1608
                     /locus_tag="VIN75_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8973118.1"
                     /translation="MTLLDKMIGTVREPLEDRRARARELAYARAAECDWFAEVLRHHV
                     AIEQALQAVREAGDPVVARHALRWLSTLMKGHAIAEDTVLYPALAFDGQKAHAVSAFA
                     DESSFRIDLAGLALLEPGSEDFVDKLDALRADLALHFYEEEGAWYPALCRREGGRGNA
                     RLHMLFVEEFRRYMGVDADLL"
     gene            1757..2011
                     /locus_tag="VIN75_02800"
     CDS             1757..2011
                     /locus_tag="VIN75_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973119.1"
                     /translation="MKLSFLDVHLSQVVRGVAATASEGVLVFHVFVSPEALLELGASE
                     DTGTWVPTFHRNESLIAAHVKAMQRAAPDAPAIIESLAVS"
     gene            2446..>3334
                     /locus_tag="VIN75_02805"
     CDS             2446..>3334
                     /locus_tag="VIN75_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973120.1"
                     /translation="MDEQKDPPLDFTAGVPLADLPEGQPVAGRVGTEDALLLRRGDRV
                     HAFGALCSHYHGPLAEGLVVGDTVRCPWHHASFDLATGEAVRAPALDPIACWRVEQRA
                     GRAFVGERVVAGPRERPPVSGHPPSIVIVGGGVAGLAAAEMLRRAGYTRALTMVSADD
                     APPCDRPNLSKDFLAGTAEPDWIALRPPGFYEANGIELLLSTRVAAIDPARRVVTLDS
                     GRELAWGALLLATGADPIRMDIPGADPARVHPLRSFADSRAIVARCAGARRALVIGSS
                     FIGLEVAASLRARGLAVHVV"
BASE COUNT          462 a         1243 c         1168 g          461 t
ORIGIN      
        1 cgccgcttcg gtcgcgcaac gggggcgacc gtccgctgcg ctgacaccgt tgcatcgagg
       61 cgcggcgaag ccgtgctgtc ggtttcctca ggccccgcag gaaagcttgc agccgcccgg
      121 caagtgcgtg tgaagacggg cctgcccgta caggccccgg ccaggcgggc atggacgtcc
      181 aggcgccgcc ggcccccgcg tgatgcgcat ggacacggcg tctcccgctc ggcccgggcg
      241 gcacgcctgg tcacgtgccc gcttcttgct tcgcgatcct ggccggcgcc tttcgacacc
      301 cgtcgcacgg cgccatcgcg acaaggagcc gtcatgaccg atccccaacg accgcgcgac
      361 caggaaccac cccgccgtgc gactcccctt cagccggaca agcccggcag cggcgcccgc
      421 cgcgagcgct ccggcgagca gcccgagccg tccgcgagcc acggcggcca ggccggcgag
      481 acgcgcaagc tgcgccgcca ggaagacagc gcggtggaca acgcgcgcga aggctacgac
      541 tgagccgcgc agatgcttgc caacctacgg agaagcacat gacccaggta tgcgaagtga
      601 tgacccgcga tgtgcgcacg ctggcgccga ccgagaccct catgaaggcc gcgcagacta
      661 tggacgagct cgacgtgggc gtcatcccgg tctgcgacgg cgaccgcctg ctgggcgtcg
      721 tcaccgaccg cgacatcgtc gtgcgcggcg tcgccgccgg ctgcatggtc gaccaggcca
      781 cgctgcgcga ggtgatgagc gagccggcgc actggtgcta cgacgacgat gccgtcgagg
      841 acgccgcgcg cacgatgggc gagcaccagc tgcgccgcgt gcccgtcatc gaccacgacc
      901 ggcgcctggt cggcatcgtc gccctggccg acctcgccac gcgcgggcag gccatgtcgg
      961 ccggcgatgc gctggaggcg gtctcgcagg gcacggcgcc cgcgtcctcc ccggcggcgc
     1021 gatgaacgca cgtgccacgg ccagcagccg ccggagtgcg ccatgacgct gctggacaag
     1081 atgatcggca ccgtgcgcga gccgctcgag gatcgccgcg cccgcgcgcg cgagctcgcc
     1141 tacgcgcgcg cggccgagtg cgactggttc gccgaggtgc tgcgccacca cgtggccatc
     1201 gagcaggcgc tgcaggcggt gcgcgaggcg ggcgatcccg tggtggcccg gcatgcgctg
     1261 cgttggctgt ccacgctgat gaagggccac gcgatcgccg aggacaccgt gctctatccg
     1321 gcgctcgcct tcgacggcca gaaggcgcac gcggtcagcg cgttcgccga cgagagcagc
     1381 ttccgcatcg acctggccgg actcgcgctg ctggagccgg gcagcgaaga cttcgtcgac
     1441 aagctggacg cgctgcgtgc cgacctggcg ctgcacttct acgaggagga gggcgcctgg
     1501 tacccggcgc tgtgccggcg cgagggcggg cgcggcaacg cgcggctgca catgctgttc
     1561 gtcgaggagt tccgccgcta catgggggtc gacgcggacc tgctctgaag ccggcgccgg
     1621 ccggctccgg tgacactggc gcttttactg atcgatcgtc cagtacgcgg cagcaacacc
     1681 cagcgcgacg gtacttccgc ggccctcgct ttcccgccag aatggacatt tgacccattt
     1741 accggaaagc gcgtccatga agctgtcgtt cctcgacgtg cacctgtcgc aggtcgtccg
     1801 gggcgtcgcc gcgacggcga gcgagggcgt cctcgtcttc cacgtgttcg tctcgcccga
     1861 ggcgctgctc gagctcggcg cgagcgagga cacgggcacc tgggtgccca cgttccatcg
     1921 caacgagtcg ctgatcgcgg cccacgtcaa ggccatgcag cgggccgcgc cggacgcgcc
     1981 ggccatcatc gagtcgctcg cggtcagctg atcgctcgtt ccggcaccct ggttgcctcg
     2041 acgagtccat ggcgccattc cggcgcgaac ctcaacggag acaaacatgg gcaagtacgt
     2101 actcggatgg ttcctgggca ttcccctcgg cgtcctcgtc atcctctacc tgatcttcca
     2161 ctaggcttct gcgcgcagac tcctggccat cggatgcgcg ctcgtcctac acaccgcgcg
     2221 ccgcgaagcg actacattgg aatcgtcccg acgacgcctc gcgccgaagg ggcaacggga
     2281 agcccgctcc aggaatggac tgcgggcttt cttctttccg gccgccggtg atcgacgccg
     2341 ccgcacgccg ggaacggaat ccgcggacaa gccatctctg cctcaagggc gcgatgcgcg
     2401 acctatgatg gccgcggcac ggcgccgctt ctcgaggaga ccgcgatgga cgagcagaag
     2461 gatccgcccc tggacttcac cgccggcgtg ccgctcgccg acctgcccga gggccagccc
     2521 gtggccggcc gcgtcggcac cgaggacgcg ctgctgctgc ggcgcggcga ccgtgtccac
     2581 gcgttcggcg cgctgtgcag ccactaccac ggcccgctcg ccgagggcct cgtcgtcggc
     2641 gacaccgtgc gctgcccctg gcaccacgcc agcttcgacc tcgccaccgg cgaggccgtg
     2701 cgggcgcccg cgctcgaccc gatcgcatgc tggcgcgtgg agcagcgcgc tggccgcgcg
     2761 ttcgtcggcg agcgcgtcgt cgccgggccg cgcgaacggc cgccggtctc cgggcatccg
     2821 ccgtccatcg tgatcgtcgg cggcggcgtc gcgggcctgg cggccgccga gatgctgcgc
     2881 cgcgccggct acacccgggc gctgaccatg gtgagtgccg acgacgcgcc gccgtgcgac
     2941 cggccgaacc tgtccaagga cttcctcgcc ggcaccgccg agcccgactg gatcgcgctg
     3001 cgcccgcccg ggttctacga ggcgaacggc atcgagctgc tgctgtccac gcgcgtcgcg
     3061 gcgatcgatc cggcgcgccg cgtcgtcacg ctggactccg gccgcgagct cgcatggggc
     3121 gcgttgctgc tggccaccgg cgccgatccg atccgcatgg acatccccgg cgccgacccg
     3181 gcgcgcgtgc acccgctgcg cagcttcgcc gacagcaggg cgatcgtcgc gcgctgcgcc
     3241 ggcgcgaggc gcgcgctggt gatcggctcg agcttcatcg gcctggaggt ggccgcgtcg
     3301 ctgcgtgccc gcggcctcgc ggtgcacgtg gtcg
//
LOCUS       DATCRO010000104         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_406121, whole genome shotgun sequence.
ACCESSION   DATCRO010000104 DATCRO010000000
VERSION     DATCRO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406121"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1611)
                     /locus_tag="VIN75_02810"
     CDS             complement(220..1611)
                     /locus_tag="VIN75_02810"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEY8973121.1"
                     /translation="MLILRHADILVTMDATRREIADGAVVCDGPAIAWAGPTADLPIA
                     CLEALRDGRAEAIDMRGHVVMPGLVNTHHHMYQSLTRAVPEAQDAELFGWLTNLYLLW
                     ARLTPEMIRVSTQTAMAELMLSGCTTTSDHLYLFPNGSRLDDSIEAADAMGMRFHAAR
                     GSMSVGRSAGGLPPDEVVETEEAILRDSERLIGRWHDASRHSMRRIVLAPCSPFSVSR
                     DLMRLSAELARERGVSLHTHLAENDNDVAYSREKFGMTPAEYAEDLGWVGRDVWHAHC
                     VKLDEAGIALFGRTGTGVAHCPCSNMRLASGIAPIGAMRRAGVPVGLGVDGSASNDGA
                     HMLGEARQAMLLQRVGYGPAAMTAREALEIATLGGAQVLGRDDIGAIAPGMSADIVAF
                     DMRGVAHAGAGHDPVAALVFCTPASVSLSVIGGVVRIRGGAFTGLELAPLLARHRELA
                     RTLYDAARHPHTA"
     gene            complement(1667..2566)
                     /locus_tag="VIN75_02815"
     CDS             complement(1667..2566)
                     /locus_tag="VIN75_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY8973122.1"
                     /translation="MPVTLEDKLVVAISSRALFNLEEENKVFEAGDNEGYMKLQLDRI
                     DVPAAPGIAYSLIRKLLRFNDDGVQRVEVVILSRNDPVSGMRIFRSSAAADIKLLRGV
                     FTQGRPPFGYLRPLRAHLFLSVNAQDVREALNAGFPAARVLVESVKASDAWPNEVRIA
                     FDGDAVLFSDEAERVFQAQGLDAFQAHELSKADLPLPEGPFKPLLAALHRLQMAGNAQ
                     MRIRTALVTARSAPAHERAIRTLMKWNIRVDEAMFLGGLPKGEFLREFEPDFFFDDQT
                     GHVDAASRHVPAGHVSSGISNES"
     gene            complement(2673..3911)
                     /gene="gspF"
                     /locus_tag="VIN75_02820"
     CDS             complement(2673..3911)
                     /gene="gspF"
                     /locus_tag="VIN75_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY8973123.1"
                     /translation="MPAYAFEAIDASGQSREGVLEADTARSARSLLRAQALIPLSVKP
                     VGSEHAGGSSQRSGLGRWFGGGKRVFNSTGLAVWTRQLAGLVSSGLPLERALTALTDE
                     AESDQQRNLVASLRAEVNAGSPFARALSAHPREFSPIYTAVIGAGEQSGNLGLVLDRL
                     ADDLEERQALQQKLIGAALYPAIVTLVAIVIVIFLVSYVVPQVAQVFAGTKRSLPFLT
                     TVMLSLSAGVRNYGWWMLAGVVLVAIGARLALAQEALRLKFDAAWLRLPLVGKLSRGY
                     NAARFASTLAMLATAGVPILRALQAASETLGNRAMRADALDALVLVREGAPLASALAQ
                     KKRFPGLVSMFARLGEQTGTLPLMLQRAANQLGAEVQRRAMHLATILEPLLIVAMGGV
                     VMLIVLAVMLPIIQLNQFVK"
     gene            complement(3930..5312)
                     /locus_tag="VIN75_02825"
     CDS             complement(3930..5312)
                     /locus_tag="VIN75_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8973124.1"
                     /translation="MTSLPYAWARAQRALLTIRPEGAQLTFSPRTPQWARAEARRHHP
                     LAQEREIADDELETLLAAAYAHAGDAASVVAASENEIDLDRLMQDIPEIEDLLDTQDD
                     APVIRMINALFTQAARDGASDIHIEPYERTSSVRFRIDGTLREVVQPNRALHAALISR
                     LKIMAELDIAEKRLPQDGRISLRIGGRAIDVRVSTLPSAHGERAVLRLLDKSESRLTL
                     EAVGMQGDTLARFRHMVTQPHGIILVTGPTGSGKTTTLYAALARLDATRSNIMTVEDP
                     IEYELPGVGQTQINAKIELTFAKALRAILRQDPDVIMIGEIRDFETAQIAIQASLTGH
                     LVLATLHTNDSVSAVTRLTDMGVEPFLLSSSLLGVLAQRLVRKVCTVCAGAGCEACGQ
                     TGYQGRTGIFELLVADDKVQALIHGKAAESQLFEAGQHGGLRSMREDGERLVQAGITS
                     RAELLRVTRE"
BASE COUNT          759 a         1822 c         1934 g          821 t
ORIGIN      
        1 caggccgccc gccgaggcgg accatttgag gatgtctctg cgcttcatcg ggagttccct
       61 gttgttgtgt atctcgggaa ggccgacgga aggctcggcc accgaatcga acggtcgttc
      121 gattcggtgg gcggattctg cggaggggct cgggcgttgg caatcagggt tgctgcctac
      181 ttcgaggcgc gtcccctgca acgcctggtc agcgcgtcgt caggcggtat gcgggtggcg
      241 tgcggcgtcg tacagcgtgc gcgccagttc gcgatgcctc gccagcagcg gtgccagctc
      301 gagcccggtg aatgcgccgc cgcggatgcg caccacgccg ccgatcacgc tcagcgacac
      361 cgaggccggc gtgcagaaca cgagcgccgc caccgggtcg tgccccgctc ccgcgtgcgc
      421 gacgccgcgc atgtcgaatg ccacgatgtc ggccgacatg ccgggcgcga tcgcaccgat
      481 gtcgtcgcgg cccagcacct gcgcgccgcc gagcgtggcg atctcgagcg cctcgcgcgc
      541 cgtcatcgcc gccgggccgt agcccacgcg ctgcagcagc atcgcctggc gcgcctcgcc
      601 cagcatgtgc gcgccgtcgt tcgacgcgct gccgtccacg cccagcccca ccggcacccc
      661 agcgcggcgc atcgcaccga tgggcgcgat gcccgaggcc aggcgcatgt tggagcacgg
      721 gcagtgcgcc acgcccgtgc cggtgcggcc gaagagcgcg atgccggcct cgtcgagctt
      781 cacgcagtgc gcatgccaca cgtcgcggcc gacccagccc aggtcttcgg cgtactcggc
      841 cggcgtcatg ccgaacttct cgcgcgagta ggcgacgtcg ttgtcgttct cggccaggtg
      901 cgtgtgcagc gagacaccgc gctcgcgcgc cagttcggcc gacaggcgca tcaggtcgcg
      961 cgacaccgag aaaggcgagc agggcgcgag cacgatgcgg cgcatcgaat gccgcgaggc
     1021 gtcgtgccag cggccgatga gccgctcgct gtcgcgcagg atcgcttcct cggtctcgac
     1081 cacttcgtcg ggcggcaggc cgccggcgct gcggcccacg ctcatgctgc cgcgcgccgc
     1141 gtggaagcgc atgcccatcg cgtcggccgc ctcgatcgaa tcgtcgagcc gcgagccgtt
     1201 gggaaacagg tagagatgat cgctggtggt ggtgcagccc gagagcatca gctcggccat
     1261 ggcggtctgc gtcgacacgc ggatcatctc gggcgtgagg cgcgcccaca gcaggtacag
     1321 gttggtcagc cagccgaaga gctcggcgtc ctgtgcctcg ggcaccgcgc gcgtgaggct
     1381 ctggtacatg tggtggtgcg tgttgaccag gccgggcatc accacgtggc cgcgcatgtc
     1441 gatggcctcg gcgcggccgt cgcgcagggc ttcgaggcat gcgatgggca gatcggcggt
     1501 ggggccggcc caggcgatcg cggggccgtc gcacaccacg gcgccatcgg cgatctcgcg
     1561 ccgggtggcg tccatggtga cgaggatgtc ggcgtgtcgg aggatcagca tggcggcatt
     1621 cttgccgcgg cccgggtcgc gggcaagcgc aaggcgacgc cgccgttcag gactcgttgc
     1681 tgatcccgct gctcacatgc cccgccggca cgtgccgaga ggccgcatcc acgtgccccg
     1741 tctggtcgtc gaagaagaag tcgggctcga actcgcgcag gaactcgccc ttgggcaggc
     1801 cgccgaggaa catggcctcg tcgacgcgga tgttccactt catcagcgtg cggatcgcgc
     1861 gctcgtgcgc cggggcgctg cgcgcggtga ccagcgcggt gcggatgcgc atctgcgcgt
     1921 tgccggccat ctgcaggcgg tgcagggcgg ccagcagcgg cttgaagggg ccttcgggca
     1981 gggggaggtc ggccttgctg agttcgtggg cctggaaggc gtcgaggccc tgggcctgga
     2041 acacgcgctc ggcctcgtcg gagaacagca cggcgtcgcc gtcgaaggcg atgcgcactt
     2101 cgttcggcca tgcgtcgctg gccttcaccg attcgaccag cacgcgcgcc gccggaaagc
     2161 cggcgttgag cgcctcgcgc acgtcctgcg cgttgaccga gaggaacagg tgcgcgcgca
     2221 gcgggcgcag gtagccgaag ggcggtcggc cctgggtgaa cacgccgcgc agcagcttga
     2281 tgtcggccgc ggcgctcgac ctgaagatgc gcatgcccga gaccgggtcg ttgcgcgaga
     2341 ggatcaccac ctccacgcgc tgcacgccgt cgtcgttgaa gcgcagcagc ttgcggatca
     2401 gcgagtaggc gatgccgggc gcggccggca cgtcgatgcg gtcgagctgc agcttcatgt
     2461 agccctcgtt gtcgcccgcc tcgaagacct tgttctcttc ctcgaggttg aacagggcgc
     2521 gcgaggagat cgcgaccacg agcttgtctt ccagcgttac cggcatggcg cttccttgcg
     2581 gctgtgtctt cttcgagcag ttgcgccgcg cgctgctgcc ccctgcaagc gggttggagg
     2641 ccgcacgaag tgggcaagcc tgggggtctg cctcatttca cgaactggtt gagctggatg
     2701 atgggcagca tcaccgcgag cacgatcagc atcaccacgc cgcccatcgc cacgatcagc
     2761 agcggctcca ggatggtggc caggtgcatc gcgcggcgct gcacttcggc gccgagctgg
     2821 ttggcggcgc gctgcagcat cagcggcagg gtgccggtct gctcgccgag gcgggcgaac
     2881 atcgacacca ggcccggaaa gcgcttcttc tgcgccagcg ccgatgccag cggcgcgcct
     2941 tcgcgcacca gcaccagcgc gtcgagcgca tcggcgcgca tcgcacggtt gcccagcgtt
     3001 tcggaggcgg cctgcagcgc gcgcaggatc ggcacgccgg cggtggccag catggcgagc
     3061 gtgctcgcga agcgcgccgc gttgtagccg cgcgagagct tgcccaccag cggcagcctg
     3121 agccaggccg catcgaactt caggcgcagc gcctcctgcg ccagcgcgag ccgtgcgccg
     3181 atggccacga gcaccacgcc ggccagcatc caccagccgt agttgcgaac gccggcgctc
     3241 agcgacagca tcaccgtggt gagaaaaggc agcgagcgct tcgtgcccgc gaacacctgc
     3301 gccacctgcg gcaccacgta gctcacgagg aagatcacga tcacgatcgc caccagcgtg
     3361 acgatggccg ggtacagcgc cgcaccgatc agcttctgct gcagcgcctg gcgctcttcg
     3421 aggtcgtcgg cgaggcggtc gagcacgagg cccaggttgc cgctctgctc gccggcgccg
     3481 atcacggccg tgtagatggg cgagaactcg cgcggatgcg ccgacagcgc acgggcgaag
     3541 ggggagcctg cgttgacctc ggcgcgcagc gaggcgacga ggttgcgctg ctggtccgat
     3601 tcggcttcat cggtcagcgc agtgagcgcg cgttccagcg gcaggcccga ggagacgaga
     3661 cccgcgagct gccgcgtcca caccgcgagc ccggtcgaat tgaacacgcg cttgccgccg
     3721 ccgaaccagc ggccgagacc gctgcgctgg ctggagccac cggcatgctc gctgccaacc
     3781 ggcttgaccg acagcggaat gagcgcctga gcccgcagca ggctgcgcgc gctgcgggcg
     3841 gtgtcggcct cgagaacgcc ttcgcgcgac tggccgctgg cgtcgatggc ttcgaatgcg
     3901 taggcgggca tctcttctct tcttgctcgc tactcgcgcg tgacgcgcag cagctcggcg
     3961 cgcgacgtga tgccggcctg caccagccgt tcgccgtcct cgcgcatcga gcgcaggccg
     4021 ccgtgctggc cggcctcgaa cagctggctc tcggcggcct tgccgtggat gagcgcctgc
     4081 accttgtcgt cggccaccag cagctcgaag atgccggtgc ggccctggta gccggtctgc
     4141 ccgcaggctt cgcagcccgc gccggcgcag accgtgcaga ccttgcgcac gagccgctgc
     4201 gcgagcacgc ccagaagcga cgaactcagc aggaagggct ccacgcccat gtcggtgaga
     4261 cgcgtgacgg cgctgacgga atcgttggtg tgcagcgtgg ccagcacgag gtggccggtc
     4321 agcgaggcct ggatggcgat ctgcgcggtt tcgaagtcgc gaatctcgcc gatcatgatc
     4381 acgtccgggt cctgccgaag gatggcgcgc agggccttgg cgaaggtgag ttcgatcttc
     4441 gcgttgatct gcgtctggcc cacgcccggc agctcgtact cgatcgggtc ttccaccgtc
     4501 atgatgttgc tgcgggtggc gtcgagccgg gccagcgctg cgtaaagcgt ggtggtcttg
     4561 cccgagcccg tggggccggt cacgaggatg atgccgtgcg gctgcgtgac catgtgccga
     4621 aagcgcgcca gcgtgtcgcc ctgcatgccc acggcctcga gcgtgaggcg gctctcggac
     4681 ttgtcgagca ggcgcagcac cgcgcgctcg ccgtgcgccg agggcagggt ggacacgcgc
     4741 acgtcgatgg cgcggccgcc gatgcgcagc gagatgcggc cgtcctgcgg caggcgtttc
     4801 tcggcgatgt cgagctcggc catgatcttc aggcgggaga tcagcgccgc gtgcagcgcg
     4861 cggttgggct gcaccacctc gcgcagcgtg ccgtcgatgc gaaagcgcac cgacgacgtg
     4921 cgctcgtagg gctcgatgtg gatgtcgctg gcgccgtcgc gcgcggcctg cgtgaacagc
     4981 gcgttgatca tgcggatgac gggcgcgtcg tcctgggtgt cgagcaggtc ctcgatttcc
     5041 gggatgtcct gcatcaggcg atccaggtcg atctcgtttt ccgacgccgc caccacgctg
     5101 gccgcatcgc cggcatgggc gtaggcggcg gccagcagcg tttccagctc gtcgtcggcg
     5161 atctcgcgtt cctgcgccag cggatggtgg cgccgcgcct cggcgcgcgc ccactgcggc
     5221 gtgcgcgggc tgaaggtcag ctgcgcgccc tccgggcgga tcgtcagcag ggcgcgctgc
     5281 gcgcgtgccc aggcgtatgg cagggatgtc atcgtgcggt ctccgatgcc gttgtc
//
LOCUS       DATCRO010000105         8309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_563814, whole genome shotgun sequence.
ACCESSION   DATCRO010000105 DATCRO010000000
VERSION     DATCRO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563814"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..823)
                     /locus_tag="VIN75_02830"
     CDS             complement(203..823)
                     /locus_tag="VIN75_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8973125.1"
                     /translation="MPSPNVDPSPYVGNDEIRIIEERVLAHDWYLLRKTTFEHRRRDG
                     RLQVVSRETYDRGNGVALLLFNAARGTVILIRQFRFPCYVNGLADGMLVEACAGQLDG
                     NDPLAAMRREAIEETGYDVKSPRKVFEAYMSPGSITEKLHFFVAAYEDADRRTGGGGD
                     VHEGEDIEVLELRLDAAMAMIASGQIQDGKTIMLLQHAALVGLDRL"
     gene            885..1640
                     /locus_tag="VIN75_02835"
     CDS             885..1640
                     /locus_tag="VIN75_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY8973126.1"
                     /translation="MLTTRRKKILLERLAAEGQLVARTLALEFDTSEDTIRRDLRELA
                     AEGKLQRVHGGALPASAAMGDLALREQVSTVDKLALGRYGASLVQPGQVVILDGGTTA
                     LQVARHLAPGLRATIVTHSPTVAVELARHPLVDVRMLGGRLFRHSMVNVGAEVIAAAS
                     RLRADLFFLGVTGVHPDAGLSTGDAEEAAVKRALMERAAETVVLASPEKLMAASPFVV
                     APLADASLVAVARATPERVTRALRAGGVRVRKA"
     gene            1655..2347
                     /locus_tag="VIN75_02840"
     CDS             1655..2347
                     /locus_tag="VIN75_02840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8973127.1"
                     /translation="MSSDDTLSADDRRMARYYAERAAYYERVYHKPERQPDLRRMESE
                     LAAHFAGRRVLEIACGTGWWTPHFARDCVSWLATDLNDETMALARAKPLPAGKVRFQR
                     VDAYTLEGIEDERFDAAFAGCWWSHVPLARLRPWLDTLHARLEPGSRVMMLDNSFVQT
                     SNLPISRRDAEGNTYQLRTLDDGSTYEVVKNFPTPDEAMAALGPRARDARWIAYEHYW
                     ALAYDLARAGAW"
     gene            complement(2464..3279)
                     /locus_tag="VIN75_02845"
     CDS             complement(2464..3279)
                     /locus_tag="VIN75_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973128.1"
                     /translation="MNLLRSAAALAAAVSITSITGCASEPVAPQHVDGGMRMPASGMD
                     GWQFHRVLRFGDYATSPVNPKATDLAHDCMPDCPNKTSIGSPGHVPIYRKQFDDAFRS
                     ATIKVEFDQHGPDAQMAPVRALYEGRRYSNVQMTEWMGFPTSIKGNVELVTSFTGVID
                     PLSAGQSAWHFALLSDNTTDAATAPAGWLVDDSGRSVVIRHAPFPVGTPAFVIRLARG
                     VSPGYLFDLDGQVVGSMSLFPEKTVWLRDDLAPDLRFALAGLSSALFLQPQSH"
     gene            complement(3374..5032)
                     /locus_tag="VIN75_02850"
     CDS             complement(3374..5032)
                     /locus_tag="VIN75_02850"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY8973129.1"
                     /translation="MNFDYIVVGGGSGGCVLAARLSEDPAVSVALIEAGPVDRSILIH
                     VPAGLALLAQTRTANWNFNTVPQKGLNGRTGWQPRGKVLGGSSSVNAMVYIRGHRADY
                     DHWAAEGNQGWSYDEVLPYFRRAEHNERLHDEYHGNDGPLNVMDLRSPNKYTQPFIDA
                     GREAGYRITDDFNGADQEGIGAYQVTHRNGERFSAAKAYLTPNLGRKNLTVFTNAHTT
                     RILFEGKRATGVEFRRDGRIEQIGANHEVLMAAGALQTPQILMLSGVGPGAELAKFGI
                     GVVHDLPGVGRNLHDHLDAVQVVNAPTLRETFGVSFGGIARVLKAIPEWRNRRTGMLT
                     TNFAEAGGFIKSQPSEPIPDLQLHFVIAKLVDHGRKTVFGHGYSGHVCVLRPKSRGSV
                     SLASADPMAAPLIDPNFLADPDDVARMVRGFRLLRELFQQPALKKLGGHEHPRSAGAT
                     TDAEIEQFIRGYADTIYHPVGTCRMGSGEMDVVGADLRIHGLEGIRVVDASIMPRIIG
                     GNTNAPTIMVAEKASDMIKAARLSGAAPAPQVIRAPASPESLAA"
     gene            complement(5088..6266)
                     /locus_tag="VIN75_02855"
     CDS             complement(5088..6266)
                     /locus_tag="VIN75_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEY8973130.1"
                     /translation="MSPVTASEPAAPYVDRKRHAWVLSLAVPMLVGLGPLLYRAWPHT
                     VMLWLPVIFVYCVAPLIDLALGADTSNPPEDAVPRLEADAYYRRVTFALVPLLWGAFV
                     YGAWTCMRAPLIWAGQLAVVMTTGMVGGFCINLGHELGHKKTALERWLAKVALAPTFY
                     GHFTIEHNRGHHRDVATPADPASARMGEGIWRFALREMPGAFRRAWQLERERMRVDGL
                     PFWSLRNELLQPALITVALWTSLALWLGPRVLVFLLLSSLWANFQLTTANYIEHYGLL
                     RRRGPDGRPERTQPQHSWNSNHLFSNWAVFHLQRHSDHHAHPLRRYQSLRHFDDVPQL
                     PNGYFGMFTIAYFPPLWFALMDGRLVAAVHSDASRINFDPRRRAALMRRHGLADVPAV
                     "
     gene            6394..7464
                     /locus_tag="VIN75_02860"
     CDS             6394..7464
                     /locus_tag="VIN75_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY8973131.1"
                     /translation="MSRHDIAHPPLPQPGSLVPPIYARLLRMLLQHADVDGDRVLAAA
                     GLDWDTLVKDDKRLGRDTIVRLAEGAMAATRRPWLGLDLGGGAPVSAHGALGYAVVTS
                     RDLRAAMHTLARYGTTRNDAMAWTCTDTRDGMTMQAADRTELGSVRAFVIDTVLGAIL
                     RMLETAVGQVPASLRIDLPLPTPAWRDQYQRFNVAEVRFEQPAFAIHVARRDLALPCI
                     GADAKAHASACRECEEALAEVAGASLAQRVAGLLAGVDDGAYPRMADVAARCGISPRT
                     LIRRLHEDGATFQQLLDAARQRRALWMLVHTAESMEEIASRLGYVDTSNFSRTVRRWF
                     GATPREVRESGGRPADGGAARS"
     gene            7593..>8309
                     /locus_tag="VIN75_02865"
     CDS             7593..>8309
                     /locus_tag="VIN75_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973132.1"
                     /translation="MTRPLLWVRSHKSSVAGLLLVVAGCVALLSLSRKVHHLSPRRAP
                     AGAAASTAHAGTAAGDASGLTPSEAAGTEQSARSARMRTEFENATSYLDFIGQAMSRP
                     QEGGKFYALLAWKRCNDLTQHKGVAATHTGNDAFFEGALALVQDIEKRCTGVLETYPD
                     VTTLYKIAVEQRGGRDFLLPENGRGIVTPTSRQNANADIDAALRTGDRWAAAEALQNN
                     AAFLDVGNSAGDDGVDRQLHE"
BASE COUNT         1202 a         2893 c         3024 g         1190 t
ORIGIN      
        1 gcgacgcggc gggctggacg cccgcgagga gcggccaacg acgcctgggg cctcgcaggc
       61 agccggccct gcgggtgatc ctccagaaag cccgccttcg gcgttgcgag cgcttgccgt
      121 ggcgccgcca cgtcggcgcg ctcgcgcctt gaaggcgggc tttctggagg tcacgcgggc
      181 acgcatttag tgtgaacagg ccctagagcc ggtccagacc caccagggcc gcatgctgca
      241 gcagcatgat ggtcttgccg tcctggatct ggccgctggc gatcatcgcc atcgcggcgt
      301 ccaggcgcag ctccagcacc tcgatgtcct cgccctcgtg cacgtcgccg ccgccgccgg
      361 tgcggcggtc ggcgtcctcg tacgcggcga cgaagaagtg cagcttctcg gtgatcgagc
      421 cggggctcat gtaggcctcg aacaccttgc gcggggactt cacgtcgtag cccgtctcct
      481 cgatggcctc gcgccgcatc gcggccagcg ggtcgttgcc gtccagctgt ccggcgcagg
      541 cctcgaccag catgccgtcc gccaggccgt tgacgtagca ggggaagcgg aactggcgga
      601 tcaggatgac cgtgccgcgc gcggcgttga acagcagcag tgcgacgccg ttgccgcggt
      661 cgtaggtctc gcggctgacc acctgcaggc ggccgtcgcg tcgacggtgc tcgaaggtcg
      721 tcttgcgcag caggtaccag tcgtgcgcga gcacgcgctc ctcgatgatg cggatctcgt
      781 cgttgccgac gtaggggctg ggatcgacgt tcggtgaagg catggcagaa tcgcgtagtt
      841 tcgtgcagta tcgtgcaaac gcccgcagcc cgcaaatcct tcccatgctc accacccgac
      901 gcaagaagat cctgctcgag cggctcgccg ccgagggcca gctcgtcgcc aggacgctgg
      961 cgctcgagtt cgacacctcc gaggacacca tccgccgcga ccttcgcgaa ctggcggccg
     1021 agggcaagct gcagcgcgtg cacggcggcg cgctgccggc gtcggcggcg atgggcgacc
     1081 tcgcgctgcg cgagcaggtg tccaccgtcg acaagctcgc gttgggcaga tacggcgcgt
     1141 cgctggtgca gccgggccag gtcgtgatcc tggacggcgg caccaccgcg ctgcaggtgg
     1201 cgcgccacct cgcgccgggg ctgcgcgcga cgatcgtcac gcacagcccg acggtcgccg
     1261 tcgaactcgc gcggcatcca ctggtcgacg tccgcatgct gggcggccgg ctgttccgcc
     1321 attcgatggt caacgtcggg gccgaggtca tcgccgccgc gtcgcgcctg cgcgccgacc
     1381 tgttcttcct cggcgtcacc ggcgtgcatc ccgacgcggg tctcagcacg ggcgacgccg
     1441 aggaagcggc ggtcaagcgc gcgctgatgg agcgcgcggc cgagacggtg gtgctcgcgt
     1501 cgcccgagaa gctgatggcc gcgtctccgt tcgtcgtggc gccgctggcc gatgcgagcc
     1561 tggtggccgt cgcgcgcgcg acgcccgagc gcgtgacgcg cgcgctgcgt gcgggcggcg
     1621 tgcgcgtgcg caaggcctga ctacactgcg ctgaatgagc agcgacgaca ccctgtccgc
     1681 cgacgaccgc cgcatggcgc gctactacgc cgagcgcgcc gcctactacg agcgggtcta
     1741 tcacaagccg gaacgccagc ccgatctgcg ccgcatggag tcggaacttg ccgcgcactt
     1801 cgccggccgc cgcgtgctgg agatcgcctg cggcaccggc tggtggacgc cgcatttcgc
     1861 gcgcgactgc gtgtcgtggc tggccaccga cctgaacgac gagacgatgg cgctcgcgcg
     1921 cgccaagccg ctgccggcgg gcaaggtgcg cttccagcgg gtcgacgcct ataccctgga
     1981 gggcatcgag gacgagcgct tcgacgccgc cttcgccggg tgctggtggt cgcacgtgcc
     2041 gctcgcgcgg ctgaggccct ggctggacac gctgcacgcg cgcctcgagc cgggctcacg
     2101 cgtcatgatg ctggacaaca gcttcgtgca gaccagcaac ctgccgatct cgcgccgcga
     2161 tgccgagggc aacacctacc agctgcgcac gctcgacgac ggcagcacct acgaagtcgt
     2221 caagaacttc ccgacgcccg acgaggcgat ggcggccctg ggcccgcgcg cccgcgatgc
     2281 gcgctggatc gcgtacgagc actactgggc gctggcctac gacctggccc gagctggcgc
     2341 gtggtgacgg acatcgccgc cctggccccc tgagcgcccg cccggcgacg cctgcgccgg
     2401 cctggcgcga tgcagtcgcc ggcgccacgc gccattcatt gctcgcgcgc ccctgcctgg
     2461 cgttcagtgc gactggggct gcaggaacag ggccgagctc aggcctgcca gcgcgaagcg
     2521 caggtcggga gcgaggtcgt cacgcagcca cacggtcttc tccgggaaca ggctcatgct
     2581 gccgaccacc tggccatcga ggtcgaagag gtagccgggc gacacgccgc gcgcgaggcg
     2641 gatgacgaac gcgggcgtgc cgacggggaa cggcgcgtgg cggatgacca cgctgcggcc
     2701 gctgtcgtcg acgagccagc cggccggcgc cgtcgcggcg tcggtggtgt tgtcgctgag
     2761 cagcgcgaag tgccaggccg actgtcctgc cgacagcgga tcgatgacgc cggtgaagct
     2821 ggtgaccagc tcgacgttgc ccttgatcga ggtgggaaag cccatccact cggtcatctg
     2881 gacattgctg tagcgtcgcc cctcgtacag cgcgcgcacc ggggccatct gggcatcggg
     2941 gccgtgctgg tcgaattcga ccttgatcgt ggcgctcctg aacgcgtcgt cgaactgctt
     3001 gcggtagatc ggcacatggc cgggcgatcc gatcgacgtc ttgttcgggc agtccggcat
     3061 gcagtcatgg gccagatcgg tcgccttcgg attgacgggg ctggtcgcgt agtcgccgaa
     3121 gcgcagcacg cggtggaact gccagccgtc catgccgctg gcgggcatgc gcatgccgcc
     3181 gtccacgtgc tggggcgcga cgggctcgct ggcgcagccg gtgatcgatg tgatggagac
     3241 ggcggcggcc agggccgcgg cggaacgcag gagattcatc gaatgcaatg gagtgcgggt
     3301 gcgtgattgc accacgacat ccttgccgtc gagcgacgaa gcgttgccgc cccgttgcgc
     3361 ggcggcgcgt ggatcaggcc gcgagcgact cgggcgacgc cggtgcgcgg atcacctgcg
     3421 gcgcgggcgc cgcgccggac aggcgcgcgg ccttgatcat gtcagaggcc ttctcggcca
     3481 ccatgatcgt cggcgcgttg gtgttgccgc cgatgatgcg cggcatgatc gacgcgtcga
     3541 ccacgcggat gccctcgagc ccgtggatgc gcaggtcggc gccgaccacg tccatctcgc
     3601 ccgatcccat gcggcaggtg cccaccgggt ggtagatggt gtcggcgtag ccgcggatga
     3661 actgctcgat ctcggcgtcg gtggtggcgc cggccgagcg cggatgctcg tggccgccca
     3721 gcttcttgag cgccggctgc tggaacagct cgcgcagcag ccggaagccg cgcaccatgc
     3781 gcgcgacgtc gtcggggtcg gccaggaagt tcgggtcgat cagcggcgcg gccatcgggt
     3841 cggcgctggc cagcgagacg ctgccgcggc tcttggggcg cagcacgcac acgtggcccg
     3901 agtagccgtg gccgaacacg gtcttgcggc catggtcgac gagcttggcg atgacgaagt
     3961 gcagctgcag gtcggggatg ggctccgacg gctggctctt gatgaagccg ccggcctcgg
     4021 cgaagttcgt cgtgagcatg ccggtgcggc ggttgcgcca ctcggggatc gccttgagca
     4081 cgcgcgcgat gccgccgaac gacacgccga aggtctccct gagcgtgggc gcgttgacga
     4141 cctgcacggc gtcgaggtgg tcgtgcaggt tgcggccgac gcccggcagg tcgtggacga
     4201 cgccgatgcc gaacttcgcg agctccgcgc cggggcccac gcccgacagc atcaggatct
     4261 gcggcgtctg cagcgcgccg gcggccatca gcacctcgtg gttggcgccg atctgctcga
     4321 tccggccgtc gcggcggaac tcgacgcccg tcgcacgctt gccctcgaac aggatgcgcg
     4381 tggtgtgcgc gttcgtgaac accgtgaggt tcttgcgacc caggttgggc gtcaggtagg
     4441 ccttggccgc gctgaagcgc tcgccgttcc tgtgcgtgac ctggtaggcg ccgatgcctt
     4501 cctggtcggc gccgttgaag tcgtcggtga tgcggtagcc cgcttcgcgg ccggcgtcga
     4561 tgaagggctg cgtgtatttg ttcgggctgc gcaggtccat cacgttgagc gggccgtcgt
     4621 tgccgtggta ctcgtcgtgc agccgctcgt tgtgctcggc gcggcggaag taaggcagca
     4681 cctcgtcgta ggaccagccc tggttgccct cggcggccca gtggtcgtag tcggcccggt
     4741 ggccgcggat gtagaccatc gcgttgaccg agctcgaccc gcccagcacc ttgccgcgcg
     4801 gctgccagcc ggtgcgcccg ttcaggccct tctgcggcac ggtgttgaag ttccagttgg
     4861 ccgtcctggt ctgcgccagc agcgccaggc ccgcgggcac gtggatgagg atgctcctgt
     4921 ccaccggccc ggcctcgatc agcgccaccg acaccgccgg gtcctcgctg agccgcgcgg
     4981 ccagcacgca gccgcccgag ccgccgccca ccacgatgta gtcgaagttc atgcttgtct
     5041 ctttcttgtg gtcagccaag gaacaccctc agggacacat gcgccgttca aacggcggga
     5101 acgtcggcaa ggccatgtcg gcgcatcagc gcggcacgtc gccggggatc gaaattgatg
     5161 cgcgacgcgt ccgagtggac cgccgcgacg aggcggccgt ccatcagggc gaaccacagc
     5221 gggggaaagt acgcgatggt gaacatgccg aagtagccgt tgggcagctg cggcacgtcg
     5281 tcgaaatgac gcagcgactg gtagcgccgc agcggatgcg cgtggtggtc ggagtggcgc
     5341 tgcaggtgga acaccgccca gttcgagaac aggtggttgc tgttccacga gtgctgcggc
     5401 tgcgtgcgct cgggccggcc gtccgggccg cggcggcgca gcaggccgta gtgctcgatg
     5461 tagttggcgg tggtcagctg gaagttggcc cacagcgagg acagcagcag gaacacgagc
     5521 acccgcgggc ccagccacag cgcgagcgac gtccacagcg ccacggtgat cagcgcgggc
     5581 tgcagcagct cgttgcgcag cgaccagaac ggcagtccgt cgacgcgcat gcgttcccgt
     5641 tcgagttgcc acgcgcggcg gaacgcgccg ggcatctcgc gcagcgcgaa gcgccagatg
     5701 ccttcgccca tgcgcgcgga cgcgggatcg gccggcgtgg ccacgtcgcg gtgatggccg
     5761 cggttgtgct cgatcgtgaa gtggccgtag aaggtcggcg ccagcgcgac cttggcgagc
     5821 cagcgctcga gcgccgtctt cttgtggccc agctcgtggc cgaggttgat gcagaagccg
     5881 cccaccatgc cggtggtcat cacgacggcc agctggccgg cccagatcag cggcgcgcgc
     5941 atgcaggtcc acgcgccgta gacgaacgcg ccccacagca gcggcaccag cgcgaaggtg
     6001 acgcggcggt agtacgcgtc ggcctccagg cgcggcacgg cgtcctcggg cgggttgctg
     6061 gtgtcggcgc ccagcgcgag gtcgatcagg ggcgcaacgc agtagacgaa gatcaccgga
     6121 agccacaaca tcacggtgtg cggccacgcg cggtacagca gcgggcccag gcccaccagc
     6181 atcggcacgg ccagcgacag cacccacgcg tggcgcttgc ggtccacgta gggcgcggcc
     6241 ggttcggatg cggtgacggg gctcatcgtg gcggccagtt tgggcgggcg gcccccgggc
     6301 cgacaccgat ggagctgcca cgaaatcgtc ataaagtgac aaacagggcc gttcaaggga
     6361 agacaccgag tccatccccg ccatgtgatt gacatgtccc gccacgacat cgcccacccg
     6421 ccgctgccgc aacccggctc gctcgtgccg ccgatctacg cgcggctgct gcgcatgctg
     6481 ctgcagcacg cggacgtcga cggggaccgc gtgctggctg cggcgggcct cgactgggac
     6541 acgctcgtca aggacgacaa gcgcctgggc cgcgacacca tcgtgcgcct ggccgaaggc
     6601 gcgatggccg ccacgcgccg gccgtggctg ggactggacc tgggcggcgg cgcgccggtg
     6661 tcggcccacg gcgcgctcgg ctacgccgtc gtcaccagcc gcgacctgcg cgcggccatg
     6721 cacacgctgg cgcgctacgg caccacgcgc aacgacgcga tggcctggac gtgcaccgac
     6781 acccgcgacg gcatgacgat gcaggccgcc gaccgcaccg agctgggcag cgtgcgcgcc
     6841 ttcgtcatcg acaccgtgct cggcgcgatc ctgcgcatgc tggagacggc cgtcggccag
     6901 gtgcccgcga gcctgcgcat cgatctgccg ttgcccacgc ccgcctggcg cgaccagtac
     6961 cagcgcttca acgtcgccga ggtgcgcttc gagcagccgg cgttcgcgat ccatgtcgcg
     7021 cggcgcgacc tcgcgctgcc ctgcatcggc gccgacgcga aggcgcacgc gagcgcctgc
     7081 cgcgagtgcg aggaggcgct ggccgaggtc gccggcgcga gcctggcgca gcgcgtcgcg
     7141 gggctgctgg ccggcgtcga cgatggcgcc tacccgcgca tggccgacgt cgccgcgcgc
     7201 tgcggcatct cgccgcgcac gctgatacgc cgcctgcacg aggacggcgc gaccttccag
     7261 cagctgctgg acgcggcgcg ccagcggcgc gcgctgtgga tgctggtgca cacggccgag
     7321 tccatggagg agatcgcctc gcgcctgggc tacgtcgaca cctccaactt cagccgcacc
     7381 gtgcgacgct ggttcggcgc gacgccgcgc gaggtgcgcg agtccggcgg acggccggcg
     7441 gacgggggcg cggcgcggtc gtagagaatg gcggccggag ccccgagtcc cgtgacgatc
     7501 cgcatgaacc cgatcccgtt cgacttccgt cacccgtgtg ccgccgcgcg cgcccacgcg
     7561 catgaggcgc gaccccgcgc gagggcgcgc gcatgacccg gccgctgctg tgggtccgct
     7621 cgcacaagtc gtcggtcgcc ggcctgctgc tggtggttgc cggctgcgtg gcgctgctgt
     7681 cgctctcgcg caaggtgcac cacctgagcc cgcgtcgcgc gcccgccggc gcggcggcct
     7741 cgacggcgca cgccgggacc gccgccggag acgccagcgg cctgacgccg tcggaagccg
     7801 cgggcaccga gcagagcgcg cgctcggccc gaatgcgcac cgagttcgag aacgcgacga
     7861 gctacctcga cttcatcggc caggccatga gccgcccgca ggagggcggc aagttctacg
     7921 cgctgctcgc ctggaagcgt tgcaacgacc tgacccagca caagggcgtg gcggccacgc
     7981 acacgggcaa cgacgcgttc ttcgagggcg cgctcgcgct cgtgcaggac atcgagaagc
     8041 gctgcaccgg cgtgctggag acctaccccg acgtgacgac gctgtacaag atcgccgtgg
     8101 agcagcgcgg cggacgcgat ttcctgctgc cggagaacgg gcgcggcatc gtcacgccga
     8161 cgtcgcgcca gaacgccaac gccgacatcg acgccgcgct gcgcacgggc gaccgctggg
     8221 ccgcggccga ggcgctgcag aacaacgccg cgttcctgga cgtcggcaat tcggcggggg
     8281 acgacggcgt ggaccgccaa ctgcacgaa
//
LOCUS       DATCRO010000106         8300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_698756, whole genome shotgun sequence.
ACCESSION   DATCRO010000106 DATCRO010000000
VERSION     DATCRO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698756"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VIN75_02870"
     CDS             complement(<1..911)
                     /locus_tag="VIN75_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY8973133.1"
                     /translation="MGFQQGLSGLSAASKNLEVIGNNVANSNTYGEKSSRAEFSDMYA
                     AAMTGSASNNTQAGIGVMVTTVAQQFNQGNITPTGNPLDLAINGNGFFQTRDASNQIQ
                     YTRNGQFKVSNEGNIVNDQGNKLLGYPADAQGNIVQGAAQPLQLPTGGIDPQATTKST
                     VEFNLDSRDAVTTPASGPSIDFTNQKTYNNATSVTMYDAKGQDVAVSYYFQKTGTDTF
                     DVYATANGQTVGGSAGAPSPIASLQFSADGSQLVSPSGPITLDIPASTNAAGAATLPI
                     TGIQLDMSGATEFGSNFGVTNMTQDGYT"
     gene            complement(940..1683)
                     /locus_tag="VIN75_02875"
     CDS             complement(940..1683)
                     /locus_tag="VIN75_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEY8973134.1"
                     /translation="MAISNATNPGLNALGGFGNSAATNAGAGAKAAAKAGKAAASGAD
                     ASNLGATQSAQDQSDRFLKLLVAQMSNQDPLNPMDSAQVTSQMAQISTVQGVQTLNKT
                     VSGLDNQFTQLQTMQGAALVGHDVATDGNALRVDADSHKGEGGFEIDSPATSVNVEIL
                     NPAGAVVGTVKMGPQEAGSHAFDFDVPQNAQGQKLTFRVNALNNEAPLDSRSFAFNSI
                     AAVSSANGKLAVELDDGRKVDYADVRAFY"
     gene            complement(1686..2102)
                     /gene="flgC"
                     /locus_tag="VIN75_02880"
     CDS             complement(1686..2102)
                     /gene="flgC"
                     /locus_tag="VIN75_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY8973135.1"
                     /translation="MSMMKIFDISGSAVSSQSQRLNVVASNLANVDTVAGPDGKAYKA
                     REVTFQTVLMNTPDGAPGAGVRVAGVTENQAPGRKVHDPANPSADADGYVTYSNVNAV
                     DEMVNMISASRSYQNNIEVMNTAKTLLMKTLQMGQG"
     gene            complement(2117..2536)
                     /gene="flgB"
                     /locus_tag="VIN75_02885"
     CDS             complement(2117..2536)
                     /gene="flgB"
                     /locus_tag="VIN75_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY8973136.1"
                     /translation="MNRLTNSLDFQAQALTLRAERQRLIASNIANADTPGYVARDMDF
                     ASALREATGTQAAGTTMTGETATARGHIPIGTGSTSELGLKYAAPSQTNLDSNTVDMD
                     RERASFVDNSVKYEATLRFINTDVRTMLDAMKSPSQG"
     gene            2742..3491
                     /gene="flgA"
                     /locus_tag="VIN75_02890"
     CDS             2742..3491
                     /gene="flgA"
                     /locus_tag="VIN75_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY8973137.1"
                     /translation="MRAHLALAAAPAARRLAGLALAGLAATASAQDEPGYTPDNALLK
                     QVETLARNGASAATAEQGSGVRVEVKVGKLDPRLKLAPCLRIDPYLPAGLPVWGSTRI
                     GLRCAQGAKAWNVSIPIQVSVFTLATVVKSALPPGTVLDGSMLMQSEVDIAAAPGAAV
                     PDPAMVIGRTLGRAVTAGATLRQTDLKPRQFFASGDTVRVTALGPGWQVVTEGQAVGA
                     GIEGQSARVRTDSGRTLSVRPTGDHQVEVTL"
     gene            3645..3959
                     /gene="flgM"
                     /locus_tag="VIN75_02895"
     CDS             3645..3959
                     /gene="flgM"
                     /locus_tag="VIN75_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEY8973138.1"
                     /translation="MKIGTITSTVATTAVDNDRRAVRQQSTTTSTSKVLDADDSSAQV
                     ELSPEAAMLSQAAQDPTFDQAKVDRIAQAIRDGNFSINPGAIADKLLANAQEVLGRTR
                     PN"
     gene            3974..4396
                     /locus_tag="VIN75_02900"
     CDS             3974..4396
                     /locus_tag="VIN75_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973139.1"
                     /translation="MNHPRAARPAAPAQALSPEVSALETSLATVELAIATLGETLAAR
                     DIVAIESASAALHDAMRAAMNQFAQVARGGKMPAELRNRFALANARITAQREALIRAS
                     SLVEQNLEILLPKPMTQTSVYSANGASQRGPGRVIAAS"
     gene            4861..5208
                     /locus_tag="VIN75_02905"
     CDS             4861..5208
                     /locus_tag="VIN75_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973140.1"
                     /translation="MHPNLQSALEEVEPLVESRGWIRPTPETIKAAERLLRLVEPLPR
                     HPAVQVEPSGTISFEWEAGDHGWLTLTVDDQGQLTHGAVLGEDEFTQSEAFGDTLPDW
                     AATLLQRVMRAGH"
     gene            complement(5216..6508)
                     /locus_tag="VIN75_02910"
     CDS             complement(5216..6508)
                     /locus_tag="VIN75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8973141.1"
                     /translation="MSALTEEQARTYMHKLLAAMAAAGGSDLFIANDFPPSMKVDGGM
                     KPMTTQKLTADVTRALASAMMSEKQREEFARELECNFAVSIPGTGRFRINIMVQQQSV
                     SLVCRTIAAEIPNFEKLGLPPVLKEVVMSKRGLVLVVGATGSGKSTSLAAMIDHRNRT
                     SSGHIITVEDPVEYVHQSQKSLVTHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDA
                     ETMEHAIAFAETGHLCLGTLHANSASQTIERVINFFPEERRAQLLMDLSANLRAIVSQ
                     RLVRREDGKGRAAAIEILLNTPTIAEKIFTGHFNEIKEIMGRSRELGMATFDWALFEL
                     YEQRVIGFEEAIRNADSANELRLNIKLRSKRGEPMAHGDGGLSLDMGDDEAPKADPVQ
                     ELRKQTEARHKAEDEEMARLREKRRQAEMAGAGGGQGR"
     gene            complement(6840..8090)
                     /locus_tag="VIN75_02915"
     CDS             complement(6840..8090)
                     /locus_tag="VIN75_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8973142.1"
                     /translation="MSEGTFRSLRNPGYRAWFAGSLVSNIGTWVQRTAQDWLVLTQLT
                     PRNATALGVMMALQFGPHIVLLPFTGSAADRFDRRRILVATQSAMFVLALTLGLLTLA
                     GRVQLWHVYAFALLLGCVTAFDAPASQTLVSELVGDKDLSNAVGLNSTSFNVARMVGP
                     AIAGVLLATLGTGWAFTANAVSYLAVLAALWRLRGAARHAGTHAARGGFVDGLRYVRG
                     RPDLMAILWMLFLIGTFGLNFTIFISTMAVSVFHEGASRYGLLMSIMAIGTISGSLLA
                     ARRQQPRIEILLSSATAFGIGCTLAALMPTYWLFGASLMLIGVSALTFANSTNSLLQL
                     GTEPAMRGRVMAMRLAIALGGTPIGAPIVGWVADTAGPRWALGVGAASGFAAAGVLVL
                     YLARHRHLSVRLDGWRVRVSVEPA"
     gene            complement(8087..>8300)
                     /locus_tag="VIN75_02920"
     CDS             complement(8087..>8300)
                     /locus_tag="VIN75_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973143.1"
                     /translation="ACAGADRMEARLANEELPMNPGHDAAWCLGGAFALDAAPCVLAP
                     ARIGARRVGRSNDGNPASVPEETRPR"
BASE COUNT         1187 a         2850 c         2893 g         1370 t
ORIGIN      
        1 gtgtagccgt cctgggtcat gttggtgacg ccgaagttcg agccgaactc ggtcgcgccg
       61 gacatgtcca gctggatgcc ggtgatcggc agcgtcgcgg cgccggcggc gttggtcgac
      121 gcggggatgt ccagggtgat cgggccgctg ggcgagacga gctggctgcc gtcggccgag
      181 aactgcagcg aggcgatcgg cgacggcgcg ccggcgctgc cgccgacggt ctggccgttg
      241 gccgtcgcgt agacgtcgaa ggtgtcggtg ccggtcttct ggaagtagta gctgacggcg
      301 acgtcctggc ccttggcgtc gtacatcgtc accgacgtgg cgttgttgta cgtcttctgg
      361 ttcgtgaagt cgatcgacgg gccggaggcc ggggtcgtca ccgcgtcgcg cgagtcgagg
      421 ttgaattcga cggtgctctt ggtggtggcc tgcgggtcga tgccgccggt gggcagctgc
      481 agcggctggg ccgcgccctg cacgatgttg ccctgcgcgt cggccgggta gcccagcagc
      541 ttgttgccct ggtcgttgac gatgttgcct tcgttgctga ccttgaactg gccgttgcgc
      601 gtgtactgga tctggttcga cgcatcgcgc gtctggaaga agccgttgcc gttgatggcc
      661 aggtccagcg ggttgcccgt gggggtgatg ttgccctggt tgaactgctg ggccaccgtg
      721 gtgaccatca cgccgatgcc cgcctgggtg ttgttggacg ccgaacccgt catcgcggcg
      781 gcgtacatgt cggagaactc ggcgcgcgag gacttctcgc cgtaggtgtt cgagttggcg
      841 acgttgttgc cgatcacttc gaggttcttc gaagcggcgc tcaagccgga cagaccttgc
      901 tggaagccca tggtggaatc tcctggagaa aactgttggt cagtagaagg cgcggacgtc
      961 ggcgtagtcg accttgcggc cgtcgtcgag ctccaccgcg agcttgccgt tggcgctgct
     1021 gacggctgcg atgctgttga aggcgaacga cctggagtcg agcggcgcct cgttgttgag
     1081 cgcgttgacg cggaacgtga gcttctggcc ctgcgcgttc tgcggcacgt cgaagtcgaa
     1141 ggcgtggctg ccggcttcct gcggccccat cttgaccgtg cccacgacgg cgccggccgg
     1201 attgaggatc tcgacgttga cgctggtggc cgggctgtcg atctcgaagc cgccctcgcc
     1261 cttgtgcgag tcggcgtcga cgcgcagcgc gttgccgtcg gtggcgacgt cgtggccgac
     1321 gagcgccgcg ccctgcatcg tctgcagctg cgtgaactgg ttgtccaggc cgctgaccgt
     1381 cttgttgagc gtctgcacgc cctgcacggt gctgatctgc gccatctggc tggtgacctg
     1441 cgccgagtcc atcgggttga gcggatcctg gttggacatc tgcgccacca gcagcttcag
     1501 gaagcggtcg gactggtcct gcgccgactg cgtcgcgccc aggttggagg cgtccgcacc
     1561 gctggccgcg gccttgccgg ccttggcggc ggccttggcg ccggcgccgg cgttggtggc
     1621 cgcggagttg ccgaagccgc cgagcgcgtt gagcccgggg ttggtggcgt tggagatggc
     1681 catgatcagc cttgtcccat ctgcagcgtc ttcatgagca gcgtcttggc ggtgttcatg
     1741 acttcgatgt tgttctggta ggaacgcgag gccgagatca tgttgaccat ctcgtcgacc
     1801 gcgttgacgt tcgagtaggt gacgtagccg tcggcgtccg cgctcgggtt ggccgggtcg
     1861 tgcaccttgc ggccgggggc ctggttctcg gtgacgcccg ccacgcgcac gccggcgccg
     1921 ggggcgccgt cgggcgtgtt catcagcacc gtctggaagg tgacctcgcg cgccttgtag
     1981 gccttgccgt ccgggccggc gacggtgtcg acgttggcca ggttggacgc gacgacgttc
     2041 aggcgctggc tctgcgacga gaccgcggaa cccgaaatgt cgaagatctt catcatgctc
     2101 atggttcagc tcccgatcag ccctggctgg ggctcttcat ggcgtcgagc atcgtgcgga
     2161 cgtccgtgtt gatgaagcgc agcgtggctt cgtacttgac cgagttgtcc acgaagctgg
     2221 cgcgctcgcg gtccatgtcg acggtgttgc tgtcgaggtt ggtctgcgag ggcgccgcgt
     2281 acttcaggcc cagctcgctg gtcgagccgg tgccgatcgg gatgtggccg cgggcggtgg
     2341 cggtctcgcc ggtcatggtg gtgccggcgg cctgggtgcc ggtggcctcg cgcagcgcgg
     2401 acgcgaagtc catgtcgcgc gccacgtagc cgggggtgtc ggcgttggcg atgttgcttg
     2461 cgatcaggcg ctggcgttcc gcccggagcg tgagcgcctg ggcctggaag tcgagcgagt
     2521 tggtcaagcg gttcatcggg tcaccttcgg tgttcctggc tcccgggccg gatcgcgaag
     2581 gacgcggatt ccgggcgcag ggacggccat ggagagcgat tgtggaagtc aagcctggaa
     2641 actgaaggcc gataaagcag gggaacggcc ctcaattcga agcacccgga cccggtggct
     2701 tccctaaagt ggccacatcc cctcaatccc ggacttgtcc cttgcgcgct cacctcgccc
     2761 ttgccgccgc tcccgccgcc cgccgcctcg ccggcctggc gttggccggg ctcgccgcga
     2821 cggcgtcggc ccaggacgag cccggctaca ccccggacaa cgccctgctc aagcaggtgg
     2881 agacgctggc gcgcaacggc gcgtccgccg ccacggccga gcagggttcg ggcgtgcgcg
     2941 tcgaagtcaa ggtgggcaag ctggacccgc gcctgaagct ggcgccctgc ctgcgcatcg
     3001 atccttacct gccggccggc ctgcccgtct ggggctcgac ccgcatcggc ctgcgttgcg
     3061 cccagggcgc caaggcctgg aacgtgtcga tcccgatcca ggtgagcgtc tttacgctgg
     3121 cgacggtcgt caagtcggct cttccgcccg gcacggtgct ggacggcagc atgctgatgc
     3181 agtccgaagt ggacatcgcg gccgcccccg gcgctgccgt gcccgatccg gccatggtga
     3241 tcggccgcac gctgggccgc gccgtcaccg cgggcgccac cctgcgccag accgacctca
     3301 agccgcgcca gttcttcgcc tccggcgaca ccgtgcgcgt caccgcgctg gggcccggct
     3361 ggcaggtggt caccgagggc caggccgtcg gcgccggcat cgagggccag agcgcccgcg
     3421 tgcgaaccga cagcggccgc acgctgagcg ttcgtccgac cggcgatcac caggtcgagg
     3481 tgacgctgtg accgtgaaat gcatcacgcc ggcggcgccc gcccgcgcgg ccaaggcccc
     3541 ggaaagtttc ttgaaaaata tcctaaagtt cgattcggga ctgccgaaac tgtctcatca
     3601 cgcatgggca tgaggacatt cattcccgtc ctcaggagag catcatgaaa attggcacca
     3661 tcaccagcac tgtcgcaacg accgccgtcg acaacgatcg ccgggccgtc cgccagcaga
     3721 gcacgaccac gtccacctcc aaggtgctgg acgcggacga ctcgagcgcg caggtcgaac
     3781 tctcgccgga ggccgccatg ctgtcccaag ccgcccagga tccgaccttc gaccaggcca
     3841 aggtggatcg catcgcccag gccatccgcg acggcaactt cagcatcaac cccggcgcca
     3901 tcgccgacaa gctgctggcc aacgcccagg aagtcctcgg ccgcacgcgc ccgaactgac
     3961 cggctgaccg acggtgaacc acccccgcgc cgcgcggccc gccgcgcccg cccaagccct
     4021 gtcgcccgag gtttccgccc tggaaacgtc gctggcgacg gtcgagctgg cgatcgccac
     4081 gctcggcgag accctggccg cgcgcgacat cgtcgccatc gagtcggcct ccgccgcgct
     4141 gcatgacgcc atgcgcgcgg ccatgaacca gttcgcccag gtcgcccgcg gcggcaagat
     4201 gcccgccgag ctgcgcaacc gcttcgcgct ggcgaacgcc cggatcaccg cgcagcgcga
     4261 ggcgctgatc cgcgccagct cgctggtcga gcagaacctc gagatcctgc tgcccaagcc
     4321 gatgacccag acgagcgtgt actcggccaa cggcgcgagc cagcgcgggc cgggccgcgt
     4381 gatcgcggcg agctgagcgt ctcgttcccg ccgagtccag ggccccgcgg ggccctcttt
     4441 catttggccc ttggcgcccc tttcgggtgc caggtcttgc ctggcaacgg ggcggtgcat
     4501 ggccgaccag ggcaggtcgg gcgccaggtc ttgtgtggca acacgctcag ggcgggccga
     4561 tttgtccggc aagacctggc acctccttgg cacctccttt gatttgcctg gcaagacctg
     4621 gcacttccgg caggcacttc cggcacaaaa tgcagacgcc cgatgctgtg caccgggcgt
     4681 ccctggccgg ctgaccgccg gctgtttggg tgtcaacgca attcaggacg ggtcaggatg
     4741 cgcgccttgc ggcatcaccg ttcggggtca ggtcttgaac gttgcggtgt caggccttga
     4801 atcgtgcagt cgccctgaat ttggacggtc tcgtgcctgc ggcgccacaa tcgcaagacc
     4861 atgcacccca acctgcaatc cgccctcgaa gaagtcgaac cgctcgtcga aagccgcggc
     4921 tggatccgcc ccacgcccga gacgatcaag gccgcggaac ggctgctgcg actcgtcgag
     4981 ccactgcccc gccaccccgc cgtgcaggtc gagccctcag gcacgatctc cttcgaatgg
     5041 gaggccggcg accacggctg gctgacgttg accgtcgacg accagggcca gttgacgcac
     5101 ggcgccgtgc tcggggaaga cgagttcacg cagtccgagg ccttcggcga cacgctgccc
     5161 gactgggccg ccaccctgct ccagcgcgtg atgcgcgccg ggcactgagc gccgctcagc
     5221 gaccctgccc gccgcccgcg ccggccatct cggcctggcg gcgcttctcg cgcagacggg
     5281 ccatctcctc gtcctcggcc ttgtggcgcg cctcggtctg cttgcgcagc tcctgcacgg
     5341 ggtcggcctt gggcgcctcg tcgtcgccca tgtcgagcga cagtccgccg tcgccatggg
     5401 ccatcggctc gccgcgcttg ctgcgcagct tgatgttcaa gcgcagctcg ttggcggagt
     5461 cggcgttgcg gatggcctcc tcgaagccga tcacgcgctg ctcgtagagc tcgaacaggg
     5521 cccagtcgaa ggtggccatg cccagctcgc gcgagcggcc catgatctcc ttgatctcgt
     5581 tgaagtggcc cgtgaagatc ttctcggcga tggtgggcgt gttcagcagg atctcgatgg
     5641 cggccgcgcg gcccttgccg tcctcgcggc gtaccaggcg ctgcgagacg atggcgcgca
     5701 ggttggccga caggtccatc agcagctggg cgcggcgctc ctcggggaag aagttgatca
     5761 cgcgctcgat cgtctggctg gcggagttgg cgtgcagcgt gcccaggcac aggtgccccg
     5821 tctcggcgaa ggcgatggcg tgctccatgg tctcggcgtc gcggatctcg ccgatcagga
     5881 tgacgtcggg cgcctggcgc agcgtgttct tgagcgcatg gtgccagctg tgcgtgtcga
     5941 cgcccacctc gcggtgcgtc accagcgact tctgcgactg gtgcacgtac tccaccgggt
     6001 cttccaccgt gatgatgtgg cccgacgagg tgcggttgcg gtggtcgatc atcgcggcca
     6061 gcgacgtcga cttgcccgag ccggtggcgc cgaccaccag caccaggccg cgcttgctca
     6121 tcaccacttc cttgagcacg ggcggcagtc ccagcttctc gaagttgggg atctcggcgg
     6181 cgatcgtgcg gcagaccagg ctcacgctct gctgctgcac catgatgttg atgcggaagc
     6241 gaccggtgcc gggaatcgac accgcgaagt tgcactccag ctcgcgcgcg aattcctcgc
     6301 gctgcttctc gctcatcatc gcgctcgcca gtgcgcgcgt gacgtcggcc gtcagcttct
     6361 gcgtggtcat gggcttcatg ccgccgtcca ccttcatgct gggcgggaag tcgttggcga
     6421 tgaacaggtc ggagccgccg gccgccgcca tcgcggcgag cagcttgtgc atgtaggtgc
     6481 gggcttgttc ttcggtgagg gcggacatgg ggctccgggc caggcggcca agtgtggaat
     6541 tcgcccgcaa atttgccagc gaacggggtc gggcgatccg cggaggaacg gggcgtcgcg
     6601 ggcggtcgtt ccgctcaagt gccgcgttca ggcttcgcgc attgcccagc tcatcggccg
     6661 ggccggcggc ccgatgaccc ggcaggcggc acttttcctc gctcgccgtg acttcgtgcg
     6721 gctccggggc gaacgggcac gcgaaggtgc ctggcatcgc caccatccgc gccgggtacc
     6781 ggccgatcgc gacgatgtcg gccgccgatg ttgacacctt ggccggcggc gtccgccgat
     6841 caagccggct cgaccgacac ccggacccgc cagccgtcca gcctcacgct caggtgccgg
     6901 tgccgcgcca ggtagagcac cagcacgccc gccgcagcga agcccgacgc cgcgcccacg
     6961 ccgagcgccc agcgcggccc ggcggtgtcc gcgacccagc cgacgatggg ggcgccgatc
     7021 ggcgtgccgc ccagcgcgat ggccagccgc atcgccatca cgcgcccgcg catcgccggc
     7081 tcggtgccca gttgcagcag gctgttggtg gagttggcga acgtgagcgc cgacaccccg
     7141 atcagcatca gcgacgcgcc gaacagccag tacgtgggca tcagcgcggc cagcgtgcag
     7201 ccgatgccga acgccgtggc cgacgacagc aggatctcga tgcgcggctg ctgccggcgc
     7261 gccgccagca gcgagcccga gatcgtcccg atcgccatga tcgacatgag caggccgtag
     7321 cggctcgcgc cctcatggaa cacgctgacc gccatcgtgg agatgaagat cgtgaagttc
     7381 agcccgaagg tgccgatcag gaacagcatc cacaggatcg ccatcaggtc gggccgcccg
     7441 cgcacgtagc gcaggccgtc gacgaagccc ccgcgcgccg cgtgcgtgcc cgcgtgacgc
     7501 gccgccccgc gcaggcgcca cagcgccgcc agcaccgcga ggtaggacac ggcgttggcc
     7561 gtgaaggccc agcccgtgcc cagcgtcgcc agcagcacgc ccgcgatcgc cggccccacc
     7621 atgcgcgcga cgttgaacga cgtggagttc aggcccacgg cgttggacag gtccttgtcg
     7681 cccaccagct ccgagaccaa ggtctggctg gccggcgcgt cgaacgcggt gacgcacccg
     7741 agcagcagcg cgaacgcgta cacgtgccac agctgcaccc tcccagccag ggtcagcagg
     7801 cccagcgtga gcgccagcac gaacatcgcc gactgcgtgg ccacgaggat gcggcggcgg
     7861 tcgaagcgat cggccgccga gccggtgaac ggcagcagga cgatgtgcgg cccgaactga
     7921 agcgccatca tgacgcccag cgccgtggcg ttgcgcggcg tcagctgcgt cagcaccagc
     7981 cagtcctgcg ccgtgcgctg cacccaggtg ccgatgttgg acaccagcga gcccgcgaac
     8041 cacgcgcggt agcccgggtt gcgcagcgag cggaacgtgc cttcgctcat cgcggtcggg
     8101 tctcctccgg caccgacgcc gggttgccgt cattggagcg accgacgcgc cgcgcgccga
     8161 tccgcgccgg cgcaagcacg cacggggcgg cgtccagcgc gaaggcgccg cccagacacc
     8221 aggcggcgtc gtgaccggga ttcattggga gttcctcgtt ggcaagtctc gcctccattc
     8281 tgtccgcgcc ggcgcaggcc
//
LOCUS       DATCRO010000107         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_608823, whole genome shotgun sequence.
ACCESSION   DATCRO010000107 DATCRO010000000
VERSION     DATCRO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608823"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..3048
                     /locus_tag="VIN75_02925"
     CDS             121..3048
                     /locus_tag="VIN75_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973144.1"
                     /translation="MKNSTGKRSAASFKLKPTMVFAGQAVAGAYIAFAATGVYAQAEP
                     AAAAPAPGAAASGAASDTQQVVVTGIRKGIEAAISVKKNSDQIVEAISAEDIGKLPDT
                     TIAESLARLPGVTAQRDKSGNATNVSIRGLGPDFNGYLLNGREQTSTGDSRADDLSVY
                     PAELIAGATVYKTGDASLMTAGLAGTIDNKLIDPLMMPKRMVAVSYEKDRTGVGLHDL
                     GHGNKYSLIYVDQFADRKIGVALGFVHKFGRTNEVGGGNWGDGGHQAFDANGNNLGTF
                     VPATVDANGNTVPAHYTDVNLPGFGSGISLQNTHKRDDRDGLAGVLEFKPNANFTSEI
                     DFYHAKILTSTKNAVVKFGMGNLPVTNATVSNGAITAGTVQLGANPNGLIDYSENISD
                     NDTLQSFGWRNSVKLSDTWRMVADYSHNSAKRVERDIEAYAGLTSADTLSFTTPNGFG
                     VPQLAVGNPSAYTSPGSIVIRDQSGWSGTNAAQAGYDKGPTTIDKIDAKRLDFTHDLA
                     SGMFSDVQFGANQTTRTKDRAADEALIVSTTGGGYDPISFPAGSYVEQNVGGTGLNLL
                     TFDPLPGLWPGATLQRKYNNDILSKTWTVKEDVTTVYGKLDVDTEMGKIPVRGNIGLQ
                     YVYTNQSSAGYQANVGSGVTLTNPASTQTTSGTRYGNLLPSLNLTGDLGNGNLVRFGA
                     GIELARPTLTDLRNSFAVSLVPLTGSNCTDAQGNPCTVLSGSSGNPYLKPFKAKAIDL
                     SYEKYFADKEGYLSAAVFYKKLDTYIVNSSESAHDFSAQAQALGVTPSPVFGYVGPYV
                     TTVNGHGGNLKGFEITAQVPFSMLSPWLSGFGINGSFSDTTSSIQSPNTIGLNPLQPA
                     AAGTIPLTGLSHLNKKLMVYYERGGFSAFAAENYRSEYVGSVANTTVGGYPTLVFIKP
                     QRWLSAQVGYEVQSGWLKGLGVRLEGNNLNKPVYQEANYAGSITTTNKTGASVDLRVS
                     YKL"
     gene            3178..4701
                     /locus_tag="VIN75_02930"
     CDS             3178..4701
                     /locus_tag="VIN75_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8973145.1"
                     /translation="MNEPGKLDVVIVGGGTSGWMTAAAMSQVLRGKYDITLIESEEIG
                     TVGVGESTIPMIALFNSMLQIDEDEFVRETQATFKLGIEFVNWARLGDRYIHSFGTIG
                     QEVWTVDFHQYWLKQYLAGKAPELEAFSINAVACGQNKFMRPRMDMPKSPLSHIRYAF
                     QFDASLYARYLRKYAEQRGVKRIEGKITGVQQREGDGFVKSVTLQSGQVVEGGLFVDC
                     SGFRALLIEGTLKSGFEDWSHWLPCDRAVAVPSAAAAGPLTPFTRCTARGAGWQWRIP
                     LQHRVGNGHVYSSKFMSDDEATAVLLGNLDGAPLAEPRPLRFTPGRRLKSWNKNVVAV
                     GLASGFIEPLESTSIHLVQMAIAHLLTYFPSTGFDEAVADQFNRMMRLEYEWVRDFII
                     LHYKATERNDTPFWNYCREMDVPEQLRKRMALFAADGRVFRDGEELFAKTSWIQVLHG
                     QRVRPRSYHPLTDLLSEQEIAKYLDEVAGVIGACVDVMPPHEKYIADHCAAAPMRAA"
     gene            4722..5192
                     /locus_tag="VIN75_02935"
     CDS             4722..5192
                     /locus_tag="VIN75_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973146.1"
                     /translation="MFKTMTGLALATLAAATLALAGCATEPRPAKLTYETSPAGATLF
                     ENGQQIGIAPVMRTYGNADANGQIRTPVVTAVWPSGAKESFWTFLKSGDDNVTTITRP
                     AKAPNLQVDLDNAQKFAADEARRKAAALRDQARSSARCQAQMQGGGTAAIDDCN"
BASE COUNT         1001 a         1842 c         1745 g          806 t
ORIGIN      
        1 tgatctagtt ttaatacatc cgattttcgg ctcttcgcaa gtgggggtcg tttgactaca
       61 aacactgcgt atcgacggtg gcgcaccccg cgtcatccgc agaacttagg agacatgctg
      121 gtgaaaaact caacgggtaa gcgttcggcc gcttctttca agctcaagcc gaccatggtg
      181 ttcgcgggcc aggctgtcgc gggggcgtac atcgccttcg ccgcgacagg ggtctacgcg
      241 caggctgagc cggccgccgc cgcgcccgct ccgggtgcag ccgcctccgg cgcggcctcc
      301 gacacgcagc aggtcgtcgt caccggcatc cgcaagggca tcgaagccgc gatctcggtg
      361 aagaagaact ccgaccagat cgtcgaggcg atctcggccg aagacatcgg caagctgccc
      421 gacacgacaa tcgccgaatc gctggcccgc ctgcccggcg tgaccgcaca gcgcgacaag
      481 agcggcaatg ccacgaacgt gagcatccgc ggcttgggtc cagacttcaa cggctacctg
      541 ctcaacggcc gcgagcagac cagcacgggc gacagccgcg ccgacgacct cagcgtctac
      601 ccggccgagc tgatcgctgg cgccaccgtc tacaagacgg gcgacgcgag cctgatgacc
      661 gccggcctcg ccggcacgat cgacaacaag ctgatcgatc cgctgatgat gcccaagcgc
      721 atggtggcgg tcagctacga gaaggaccgc accggcgtgg gcctgcacga cctgggccac
      781 ggcaacaagt actcgctgat ctatgtcgac cagttcgccg accgcaagat cggcgtcgcg
      841 ctcggcttcg tgcacaagtt cggccgcacc aacgaggtgg gcggcggcaa ctggggcgac
      901 ggcggccacc aggccttcga cgccaacggc aataacctgg gtacctttgt tccagcgacc
      961 gtcgacgcga acggcaacac cgtccctgcg cactacaccg acgtgaacct gccgggcttc
     1021 ggcagcggca tcagcctgca gaacacgcac aagcgcgacg accgtgacgg cctggccggt
     1081 gtgctggagt tcaagcccaa cgcgaacttc accagcgaga tcgacttcta ccacgcgaag
     1141 atcctcacgt cgacgaagaa cgcggtcgtc aagttcggca tgggcaacct gcccgtgacc
     1201 aacgcgacgg tgagcaacgg cgcgatcacc gccggcaccg tgcagctcgg cgccaacccg
     1261 aacggcctga tcgactattc ggagaacatc tccgacaacg acaccttgca gtccttcggc
     1321 tggcgcaaca gcgtcaagct gtccgacacg tggcgcatgg tggccgacta cagccacaac
     1381 tcggccaagc gcgtcgagcg cgacatcgag gcctatgccg gcctgacgtc ggccgacacg
     1441 ctcagcttca ccacgcccaa cggcttcggc gtgccgcaac tggccgtcgg caatccgtcg
     1501 gcgtacacct cgcctggttc catcgtgatc cgcgaccaga gcggctggtc gggcacgaac
     1561 gcggcgcagg ccggctatga caagggcccg accacgatcg acaagatcga cgccaagcgc
     1621 ctggacttca cgcatgacct ggcctcgggc atgttcagcg atgtccagtt cggcgccaac
     1681 cagaccacgc gcacgaagga tcgcgccgcg gacgaggcac tgatcgtctc gaccaccggc
     1741 ggcggctacg accccatttc gttcccggcg ggttcgtacg tcgagcagaa cgtcggcggc
     1801 accggcctga acctgctgac cttcgatccg ctgcccggcc tgtggcccgg cgcgacgctg
     1861 cagcgcaagt acaacaacga catcctgtcc aagacgtgga cggtcaagga agacgtgacc
     1921 accgtgtacg gcaagctcga cgtcgacacc gagatgggca agatcccggt gcgcggcaac
     1981 atcggcctgc agtacgtcta caccaaccag tcgtcggccg gctaccaggc caacgtcggc
     2041 tcgggcgtga cgctgaccaa cccggcgagc acccagacga ccagcggcac gcgctacggc
     2101 aacctgctgc cgtcgctgaa cctgacgggc gacctgggca acggcaacct ggtgcgtttc
     2161 ggcgccggca tcgaactcgc ccggccgacg ctcacggacc tgcgcaactc cttcgccgtc
     2221 tcgctggtcc cgctcacggg ttccaactgc acggacgcgc aaggcaatcc ctgcacggtg
     2281 ctcagcggct cctcgggcaa cccctacctg aagccgttca aggccaaggc gatcgacctg
     2341 tcgtacgaaa agtatttcgc ggacaaggaa ggctacctga gcgcggccgt cttctacaag
     2401 aagctcgaca cctacatcgt caactcgtcc gagtcggccc acgatttctc ggcgcaggcg
     2461 caagccctgg gcgtcacccc gtcgccggtg ttcggctacg tcgggcccta cgtgacgacg
     2521 gtgaacggcc acggcggcaa cctcaagggc ttcgaaatca cggcacaggt gccgttcagc
     2581 atgctgtcgc cctggctgag cggtttcggc atcaacggca gcttctcgga taccacgagc
     2641 tcgatccagt cgcccaacac gatcggattg aatccgctgc agcctgctgc cgcgggcacg
     2701 attccgctga cggggctctc gcacctgaac aagaagctga tggtctacta cgagcgtggc
     2761 ggcttcagcg cgttcgccgc cgagaactac cgctcggaat acgtcggcag cgtggccaac
     2821 acgacggtcg gcggctaccc gacgctggtg ttcatcaagc cccagcgctg gctgtcggcg
     2881 caggtcggct acgaggtgca gtcgggctgg ctcaagggcc tgggtgtgcg cctcgagggc
     2941 aacaacctga acaagccggt gtaccaggaa gcgaactacg ccgggtcgat cacgaccacc
     3001 aacaagaccg gtgcgtcggt cgacctgcgc gtctcgtaca agctgtaagc ctgcggcgcg
     3061 gcgctgtcgt acagtcgtgc gacagcgcgc acgaaaccct tgcgcggcgc acgtcggcaa
     3121 gggttttttt caatttgcgg cgagatcggt cgacgggcct cgccaggagt ctctcccatg
     3181 aatgaaccgg gcaaactgga cgtcgtgatc gtcggcggcg gcacctccgg ctggatgacc
     3241 gcggcggcga tgtcgcaggt gctgcgcggc aagtacgaca tcacgctcat cgagtccgag
     3301 gagatcggca ccgtcggcgt cggcgagtcc acgatcccca tgatcgcgct gttcaactcg
     3361 atgctgcaga tcgacgagga cgagttcgtc cgcgagacgc aggccacgtt caagctgggc
     3421 atcgagttcg tcaactgggc ccggctgggc gaccgctaca tccacagttt cggcaccatc
     3481 gggcaggagg tgtggacggt cgatttccac cagtactggc tcaagcagta cctggccggc
     3541 aaggcgcccg agctcgaggc cttctccatc aacgcggtgg cctgcgggca gaacaagttc
     3601 atgcgcccgc gcatggacat gcccaagtcg ccgctgtcgc acatccgcta tgcgttccag
     3661 ttcgacgcgt cgctgtacgc gcgctacctg cgcaagtacg ccgagcagcg cggcgtcaag
     3721 cgcatcgaag gcaagatcac cggcgtgcag cagcgcgagg gcgacggctt cgtcaagtcg
     3781 gtcacgctgc aatcgggcca ggtggtcgag ggcggcctgt tcgtcgactg ctcgggcttc
     3841 cgcgcgctcc tgatcgaggg cacgctcaag agcggcttcg aggactggtc gcactggctg
     3901 ccgtgcgatc gcgccgtcgc ggtgcccagc gccgcggccg ccgggccgct gacgccgttc
     3961 acgcgctgca cggcgcgcgg cgcgggctgg caatggcgca tcccgctgca gcaccgcgtg
     4021 ggcaacggtc acgtgtattc cagcaagttc atgagcgacg acgaggcgac cgccgtcctg
     4081 ctgggcaacc tcgacggcgc gccgctggcc gagccgcggc cgctgcgctt cacgcccggt
     4141 cgccggctga agtcctggaa caagaacgtg gtggcggtcg gcctcgcgtc gggcttcatc
     4201 gagccgctgg agtcgaccag catccacctg gtgcagatgg ccatcgcgca cctgctgacg
     4261 tacttcccgt cgaccggctt cgacgaggcg gtggccgacc agttcaaccg catgatgcgg
     4321 ctggaatacg agtgggtgcg tgacttcatc atcctgcact acaaggcgac ggagcgcaac
     4381 gacacgccat tctggaacta ctgccgcgag atggacgtgc ccgagcagct gcgcaagcgc
     4441 atggcgctgt tcgcggccga tggccgcgtg ttccgcgacg gcgaggagct gttcgccaag
     4501 acgagctgga tccaggtgct gcacggccag cgcgtccgcc cgcgcagcta ccatccgctc
     4561 accgacctgc tcagcgagca ggagatcgcc aagtacctcg atgaggtcgc cggcgtgatc
     4621 ggggcgtgcg tcgacgtgat gccgccccac gagaaataca tcgcggatca ctgtgccgcg
     4681 gccccgatgc gggccgcatg acaaccaaag gggaagaaga aatgttcaag acgatgaccg
     4741 gcctggcgct cgccacgctc gctgccgcga ccctggcgct cgccggttgc gcgaccgagc
     4801 cgcgtcccgc caagctgacc tacgagactt cgccggctgg cgccacgctg ttcgagaacg
     4861 gccagcagat cggcattgcg ccggtgatgc gcacctacgg caatgccgac gccaacgggc
     4921 agatccgcac gccggtggtc actgccgtgt ggcccagcgg cgccaaggaa agcttctgga
     4981 cgttcctgaa gtccggcgac gacaacgtga ccaccatcac ccggccggcg aaagcgccga
     5041 acctgcaggt ggacctggac aacgcgcaga agttcgccgc cgacgaggcg cgccgcaagg
     5101 cggcagcgct gcgggaccag gcgcgttcct cggcgcgctg ccaggcgcag atgcagggcg
     5161 gaggcacggc ggcaatcgac gactgcaact gatcgccggg ggcctgccgg cgcgcgcgga
     5221 tcgcggcgtc aggccgcctt gcgccggcag ttgaagaagg cgttgagcgt cagccgggcg
     5281 gtggccggat cggtggtgag caggtccggc cggccgatgt cgccggagtg gaagaacccg
     5341 gcgtcgtaca ggatcatccg gttccatgcc gcgggcaccc gcgccacgca atcg
//
LOCUS       DATCRO010000108         6378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_113711, whole genome shotgun sequence.
ACCESSION   DATCRO010000108 DATCRO010000000
VERSION     DATCRO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113711"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIN75_02940"
     CDS             <1..702
                     /locus_tag="VIN75_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973147.1"
                     /translation="GGQAWTTHEALATTHVLELADDDGILGITHEPAFAIGQRAMLLP
                     TDAGNILWECLSVVTPEAVGALEARGGVDLIAISHPHFYAAMVEWSDALGGVPILLHA
                     ADRRWVHRSSPRIEFWSGDERALSPDVTLIRCGGHFDGSTALHWQRGPRPGGALLPGD
                     ALQVAADRRHVSFMYSYPNLIPMKPADVRAMRDRLARFEFEDVFGFSRGRNIVGGGRA
                     AVDRSFERWLGAVAA"
     gene            complement(710..1600)
                     /locus_tag="VIN75_02945"
     CDS             complement(710..1600)
                     /locus_tag="VIN75_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY8973148.1"
                     /translation="MEIWKLLLHWSAPTAQDARWHWRRPLHHVIRGTQVVWDALFAFV
                     TEEALIRASSLAFTSVLALVPLMTVGLRVMNFYGVSEATREQFEDVLAQYLLPAQSRD
                     VVNLVLQAASQVTQNIGALGLAGFCVTLVLMARELEGHIQKICGAKGSLATSVLHYAA
                     FLVLAPTGALLAFALLHPLAALLSLLPGSFGRINYPFVLSIVVLFVTLRAFSGYVLSW
                     RASAFGAIAAGIAAWGSWRGCALYFSHSVSLSAYGALASIPAFLLWVFVAWCCVLFGV
                     QVAAKAQPLCPQGRVPRLLK"
     gene            complement(1754..2905)
                     /locus_tag="VIN75_02950"
     CDS             complement(1754..2905)
                     /locus_tag="VIN75_02950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY8973149.1"
                     /translation="MTVITCIEDLRVLAQRRVPRMFYDYADSGSWTEGTYRANESDFH
                     KIRLRQRVAVNMVDRSLATTMIGVPVSMPIALAPTGLTGMQHADGEILAARAAEAFGV
                     PFTLSTMSICSIEDVAAHTKAPFWFQLYVMRDRGFIERLIERAKAAKCGALVLTLDLQ
                     ILGQRHKDVRNGLSAPPKPTLANLINLATKPRWCLGMLGTKRRTFGNIVGHVKDVGDV
                     SRLSDWTARQFDPALSWADVEWVKKLWGGKLILKGVMDVEDARLAAQSGADALVVSNH
                     GGRQLDGAPSTISALPAIVDAVGTDIEVWLDSGIRSGQDVLKAVALGAKGTMIGRPFL
                     YGLGAMGEAGVTKCLEILRKELDVTMAFCGHRDIRNVGRDILLPGTFPG"
     gene            complement(3024..3389)
                     /locus_tag="VIN75_02955"
     CDS             complement(3024..3389)
                     /locus_tag="VIN75_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8973150.1"
                     /translation="MITQIKFVGVPVSDQDRALAFYTDKLGFEVATDQAMGPGRRWIE
                     LRIGHAPTRLVLFTPPGHEDRIGTFFNGSLQCDNVEATHRQLSQRGVEFVEGPTKQPW
                     GTFAKFRDPDGNTFVLSSG"
     gene            3460..3762
                     /locus_tag="VIN75_02960"
     CDS             3460..3762
                     /locus_tag="VIN75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8973151.1"
                     /translation="MASIATLAFDAYVVDSLLPDIVGHDRKPSAFLVYLVLAGIARRT
                     GRDRVSASLETIAVKTGLSKSSVQSALRHLRRRGLVKDEPASSTDPVRTILRPWIR"
     gene            3792..4349
                     /locus_tag="VIN75_02965"
     CDS             3792..4349
                     /locus_tag="VIN75_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8973152.1"
                     /translation="MATIREGKRSVLVVVDVQVDVVRNAWERARVIGNVALAVERARA
                     QGVPVAWVQHEDDDMPRGSAGWQWVPELVPRDGDVRIFKRYPSSFEDTPLEAELARLG
                     ASRIVLAGAQTNWCIRATAYAALERGYDLVLVRDAHTTDAADLGQGRVIAAEDVVDDL
                     NLAMTWLAYPGRKPSTAKAAELDFA"
     gene            complement(4352..5278)
                     /locus_tag="VIN75_02970"
     CDS             complement(4352..5278)
                     /locus_tag="VIN75_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HEY8973153.1"
                     /translation="MARTWFSQGLEGAIARDIQANLLKQGFFAGNHDTFIDGDFGGHT
                     VAALQQLQSARSLPQTGAVDDATWGQLTVEAVPTLFERCLGLTASFEGNGYTQIEGNF
                     DGAGLTWGIIGFTLSNGEIQGLLAEIEQTAPGTLARVMGADLAAQWAAQMARPIAQQL
                     QWADSISSGPSKLGLPPEWVDAFQRLGQEPLVQRLQMHHAYDSYFVPAAATASTLGLA
                     TELGLALCFDCHVQNGKARVLAVQDLQRAGRAPTEAQQRAAFARAVASHASPTYQDDV
                     LVRKMAVATGAGTVHGRAYTLAHWGLDEFPAA"
     gene            complement(5361..>6378)
                     /locus_tag="VIN75_02975"
     CDS             complement(5361..>6378)
                     /locus_tag="VIN75_02975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8973154.1"
                     /translation="FGVFSGMGMMKAAALAPHAVLLPTDFDSYRHYSRQFKAAVAEIA
                     PVIEDRGIDEIYIDLTDVPGIREPIGHDPLGGVRAFGQELKNNVRRATGLTCSVGITP
                     NKLLSKIASELDKPNGLTVLTMDDIPTRIWPLPVRRINGIGPKAGARLDALGIHTIGE
                     LAARDMDWLVQHFTVNTAQWLHDVSRGLDERPVVTNSDPVSMSRETTFDRDLHAKRDR
                     GELGAIFTELCTQVAADLSRKGWVGRTIGIKLRFDDFKTVTRDQSIAIPTADPAEIRH
                     VAGQCLKRVDLTRRLRLLGVRVGGLSRPGAPASAPRARPKAAGQASTATPQPAQRPDE
                     PTLF"
BASE COUNT          900 a         2207 c         2335 g          936 t
ORIGIN      
        1 ggcgggcagg cgtggacgac gcacgaggcg ctggcgacga cccacgtcct ggagctcgcc
       61 gacgacgacg gcatcctggg catcacccac gaaccggctt tcgcgatcgg ccagcgcgcc
      121 atgctgctgc ccaccgatgc cggcaacatc ctgtgggagt gcctgagcgt cgtgacgccc
      181 gaggccgtcg gggcgctcga ggcgcgcggc ggcgtcgacc tgatcgcgat ctcgcatccg
      241 cacttctacg ccgcgatggt ggagtggagc gacgcgctgg gcggcgtgcc catcctgctg
      301 cacgcggccg accggcgctg ggtgcatcgc tcgtcgccgc gcatcgagtt ctggagcggt
      361 gacgagcgcg cgctgtcgcc ggacgtcacg ttgatccgct gcggcggtca cttcgacggc
      421 agcaccgcgc tgcactggca gcgcggcccg cggcctggcg gggcgctgct tccgggggac
      481 gcgctgcagg tggcggccga ccgtcgccac gtgtcgttca tgtacagcta tccgaacctg
      541 atcccgatga agccggccga tgtgcgcgcg atgcgcgacc ggctcgcgcg cttcgagttc
      601 gaggacgtgt tcggcttcag ccgcggccgc aacatcgtcg gcggcgggcg cgcggcggtg
      661 gatcgctcct tcgagcgctg gctcggagcc gtcgcggcgt gacgcgtcgc tatttcagca
      721 gccgcgggac gcgcccctgc gggcacagcg gctgcgcctt ggccgccacc tgcaccccga
      781 acagcacgca gcaccaggcg acgaacaccc acagcaggaa ggccgggatg ctggccagcg
      841 caccgtaggc cgacagcgac accgagtggc tgaagtacag cgcgcagccg cgccacgagc
      901 cccaggcggc gatgcccgcg gcgatggcgc cgaacgcgct ggcgcgccag ctcagcacgt
      961 agcccgagaa cgcgcgcagc gtcacgaaca gcaccacgat gctcagcacg aacgggtagt
     1021 tgatgcggcc gaagctgcct ggcagcagcg acagcagcgc cgccagcgga tgcagcagcg
     1081 cgaacgcgag cagcgcgccg gtcggcgcca gcaccaggaa cgcggcgtag tgcagcacgc
     1141 tggtcgccag cgaccccttg gcgccgcaga tcttctggat gtggccctcg agctcgcgcg
     1201 ccatcagcac cagcgtcacg cagaagcccg ccaggcccag cgcgccgatg ttctgcgtga
     1261 cctgcgacgc cgcctgcagc accaggttga cgacgtcgcg gctctgggcg ggcagcaggt
     1321 actgcgcgag cacgtcctcg aactgctcgc gcgtggcctc ggacacgccg tagaagttca
     1381 tcacgcgcag gcccacggtc atcagcggca ccagcgccag caccgaggtg aaggccagcg
     1441 agctggcgcg gatcagagcc tcctcggtga cgaaggcgaa cagggcgtcc cagaccacct
     1501 gggtgccccg gatgacgtga tgcagcggcc ggcgccagtg ccagcgggcg tcctgcgcgg
     1561 tgggcgcgga ccagtgaagc agcagcttcc agatttccat cgatgaaggg gctgggcaag
     1621 gacgtgcgcc gcaccggcgg cgcgaggcgc gtcggctgca gtggcctgtc aggacgacgg
     1681 gcggttggag tggcgattct gcgccgagtt ccgtgacgtc acgcgcggcc atgccgtggc
     1741 cggcgcaccg cgctcacccg gggaacgttc ccggcagcag gatgtcccgc cccacgttgc
     1801 ggatgtcgcg gtggccgcag aaggccatcg tgacgtccag ctccttgcgc aggatctcca
     1861 ggcacttggt gacgccggcc tcgcccatcg cgcccaggcc gtacaggaac ggccggccga
     1921 tcatcgtgcc cttggcgccg agcgcgacgg ccttgagcac gtcctggccc gagcggatgc
     1981 ccgagtcgag ccagacctcg atgtccgtgc cgaccgcgtc gacgatggcc ggcagcgccg
     2041 agatcgtgct gggcgcgcca tcgagctggc ggccgccgtg gttggagacg accagcgcgt
     2101 cggcgcccga ctgcgcggcc aggcgcgcgt cctcgacgtc catcacgccc ttgaggatca
     2161 gcttgccgcc ccacagcttc ttgacccatt cgacgtcggc ccagctcagc gccgggtcga
     2221 actgcctggc ggtccagtcg gacaggcggc tgacgtcgcc cacgtccttc acgtggccga
     2281 cgatgttgcc gaacgtgcgg cgcttcgtgc ccagcatgcc caggcaccag cgcggcttgg
     2341 tcgcgaggtt gatcaggttg gccagcgtgg gcttgggcgg cgcggacagg ccgttgcgca
     2401 cgtccttgtg gcgctggccg aggatctgca ggtccagcgt gagcaccagc gccccgcact
     2461 tcgcagcttt cgcgcgctcg atcaggcgct cgatgaagcc gcggtcgcgc atcacgtaga
     2521 gctggaacca gaacggcgcc ttggtgtggg ccgcgacgtc ctcgatcgag cagatgctca
     2581 tcgtcgacag cgtgaacggg acgccgaagg cctcggcggc gcgcgcggcc aggatctcgc
     2641 cgtcggcgtg ctgcatgccg gtcaggccgg tgggcgcgag cgcgatgggc atggacacgg
     2701 gcacgccgat catcgtcgtg gccaggctgc ggtcgaccat gttgaccgcc acgcgctggc
     2761 gcagccggat cttgtggaag tcgctctcgt tggcacggta ggtgccttcc gtccacgagc
     2821 ccgagtcggc gtagtcatag aacatgcgcg gcacgcgcct ttgcgccagc acgcgcaggt
     2881 cttcgatgca ggtgatcacg gtcatcgtgg ttctcgtcct ccgaggcagc agggatggta
     2941 gcgcgggcag ggtgctcgcg tcgtcgcgca cgcgaccgcg gcgtgcggct ccggggcagc
     3001 atgtgaaccg cgaggggccc cgctcaaccc gaggacagga cgaacgtgtt gccgtcggga
     3061 tcgcggaact tcgcgaaggt gccccagggc tgcttcgtcg gcccttcgac gaactcgacg
     3121 ccgcgctgcg acagctggcg gtgcgtcgcc tcgacgttgt cgcactgcag cgagccgttg
     3181 aagaacgtgc cgatgcggtc ctcgtggccg ggcggcgtga acagcaccag gcgcgtcggg
     3241 gcgtggccga tgcgcagttc gatccagcgc cgcccggggc ccatcgcctg gtcggtggcg
     3301 acctcgaagc cgagcttgtc cgtgtagaag gcgagcgcgc gatcctggtc gctgaccggc
     3361 acgccgacga acttgatctg ggtgatcatc gcgattcctc cacgttggat gacgacgcat
     3421 tctgggcgcg ggccgggccg tggggtagta tccactgcca tggcaagcat tgctaccctc
     3481 gcattcgacg cctacgtcgt cgactccctg ttgcccgaca tcgtcggcca cgaccgcaag
     3541 ccgtcggctt tcctggtcta cctcgtcctg gcgggcatcg cgcggcgcac cgggcgtgac
     3601 cgggtcagcg cgagcctgga gacgatcgcg gtgaagacgg gcctgtcgaa gtcgtcggtg
     3661 cagtcggcct tgcgacacct gcgccgacgt ggcctggtca aggacgagcc ggcgtcgtcc
     3721 accgatcccg tgcgcacgat cctgcggccc tggatcaggt aggctgcggc atttacccgg
     3781 gaagacggaa catggccacc atccgcgaag gcaagcgcag cgtcctcgtc gtcgtcgacg
     3841 tccaggtgga cgtggtgcgc aatgcgtggg agcgggcgcg cgtgatcggc aacgtggcgc
     3901 tggccgtcga gcgggcgcgt gcgcagggcg tgcccgtggc ctgggtgcag cacgaggacg
     3961 acgacatgcc gcgcggcagc gccggctggc aatgggtgcc cgagctcgtg ccgcgcgacg
     4021 gcgacgtgcg catcttcaag cgctacccgt catcgttcga ggacacgccg ctggaggccg
     4081 aactcgcgcg cctgggcgcc tcccgcatcg tcctggccgg cgcgcagacc aactggtgca
     4141 tccgcgccac cgcgtacgcg gcgctggagc gcggctacga cctcgtgctg gtgcgcgacg
     4201 cccacaccac cgacgccgcc gacctgggcc agggccgcgt gatcgccgcc gaggatgtcg
     4261 tcgacgacct caacctcgcg atgacctggc tggcctatcc cggccgcaag ccgtcgaccg
     4321 ccaaggccgc cgagctcgac ttcgcctgat ctcaggccgc cgggaactcg tccaggcccc
     4381 agtgcgccag cgtgtaggcg cggccgtgca cggtgcccgc gccggtggcc accgccatct
     4441 tgcgcacgag gacgtcgtcc tggtacgtgg ggctcgcatg cgaggccacg gcgcgcgcga
     4501 acgcggcgcg ctgctgcgcc tcggtcggcg cgcggccggc gcgctgcagg tcctgcaccg
     4561 cgagcacccg cgccttgccg ttctgcacgt ggcagtcgaa gcacagcgcg aggcccagtt
     4621 cggtggccag gcccagcgtg ctcgcggtgg ccgcggcggg cacgaagtag ctgtcgtagg
     4681 cgtggtgcat ctgcagccgc tgcaccagcg gttcctggcc caggcgctgg aacgcgtcga
     4741 cccattcggg cggcaggccc agcttgctcg ggccgctgct gatcgagtcg gcccactgca
     4801 gctgctgcgc gatcggccgg gccatctgcg cggcccactg cgcggcgagg tcggcgccca
     4861 tcacgcgcgc cagcgtgccg ggcgccgtct gctcgatctc ggccagcaag ccctggatct
     4921 cgccgttgga cagcgtgaag ccgatgatgc cccacgtgag gccggcgccg tcgaagttgc
     4981 cctcgatctg cgtgtagccg ttgccctcga aggacgcggt caggcccagg cagcgctcga
     5041 acagcgtggg gacggcctcg accgtcagct ggccccaggt ggcatcgtcg acggcgccgg
     5101 tctgcgggag cgagcgcgcc gactgcagct gctgcagcgc ggcgaccgtg tggccgccga
     5161 agtcgccgtc gatgaacgtg tcatggttgc cggcgaagaa gccctgcttg aggaggttgg
     5221 cctggatgtc gcgcgcgatg gcgccctcca ggccttgcga gaaccaggtg cgtgccatgc
     5281 gggctccctc gttgcctgtc gtgcgcgaca gcataggccg ccggcgggcg ccgcggcgcc
     5341 gtcgagttgg ggaggcaggc tcagaacagc gtgggttcgt ccggccgctg cgcgggctgc
     5401 ggcgtggccg tgctcgcctg gccggcggcc ttgggcctgg cgcgcggcgc ggacgcgggc
     5461 gcgcccgggc gcgagaggcc gcccacgcgc acccccagca gccgcagccg ccgcgtgagg
     5521 tcgacgcgct tgaggcactg gccggccacg tggcggatct ctgccgggtc ggcggtgggg
     5581 atggcgatcg actggtcgcg cgtgaccgtc ttgaagtcgt cgaagcgcag cttgatgccg
     5641 atggtgcggc ccacccagcc cttgcgcgac aggtcggcgg ccacctgcgt gcacagctcg
     5701 gtgaagatgg cgcccagctc gccgcggtcg cgcttggcgt ggaggtcgcg gtcgaaggtg
     5761 gtctcccggc tcatcgacac cggatcgctg ttggtgacca ccgggcgctc gtcgaggccg
     5821 cgcgagacgt cgtgcagcca ctgcgcggtg ttgacggtga agtgctgcac cagccagtcc
     5881 atgtcgcgcg cggcgagctc gccgatcgtg tggatgccca gcgcgtcgag ccgcgcgccg
     5941 gccttcggac cgatgccgtt gatgcggcgc accggcagcg gccagatccg cgtggggatg
     6001 tcgtccatcg tgagcacggt caggccgttg ggcttgtcca gctcggaggc gatcttggac
     6061 agcagcttgt tgggcgtgat gcctaccgag caggtgaggc cggtggcgcg gcgcacgttg
     6121 ttcttgagct cctggccgaa cgcgcgcacc ccgcccagcg ggtcgtggcc gatcggctcg
     6181 cggatgccgg gcacgtcggt caggtcgatg tagatctcgt cgatgccgcg atcctcgatc
     6241 accggcgcga tctcggcgac ggcggccttg aactggcgcg agtagtggcg gtagctgtcg
     6301 aagtcggtgg gcagcagcac cgcgtgcggc gccagcgcgg cggccttcat catgcccatg
     6361 cccgagaaca cgccgaag
//
LOCUS       DATCRO010000109        10674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_23641, whole genome shotgun sequence.
ACCESSION   DATCRO010000109 DATCRO010000000
VERSION     DATCRO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23641"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..8407
                     /locus_tag="VIN75_02980"
     CDS             <1..8407
                     /locus_tag="VIN75_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEY8973155.1"
                     /translation="HLDPNKRYFISILPGDSVNPTIGGPGSQPVDPANPAGPQRPFDI
                     GKDCGAYPGPTTDGPWAPAGGSAMCGHGMTGAQISRELLAAANPSLTIKAQETPLPTA
                     KISVLVYQDDNPLNGENDAGGGVDVIAPNEAGLGGFEIKLFDQAGQLGDNTGQITYDE
                     FNQPVSNSLANTIDPITKLDACPISARGDGLVGMIPTCPKYESDGKTLSPLAGQAVIA
                     NLYPGLYEIQAYPAADRIGRGEEWVQTNTLDGGKPHEAFIKPNEPGYFQEFGPGGFHV
                     TIGFANPAIINNRLAAYCASPANGGGTGAQACDKKLTVHVANSHMSRSPDQRMYSSGS
                     YDNYSFTNCYVSIGPADAADFAFAKCDANGTAVFNNIPNGTYKISVFDQWNDIMLDGL
                     VGTVAVAGDTEKEFPVTQWRTNLYTRTFIDSNGDGVSQDPGELGLALVNTNIRYRDGS
                     FAFFNNTDLNGYAAWNEVFPFMNWLVVETSATRFKTLGVHSVYDAGGPVDCDVQSTIC
                     SNIADHLANTDVRVPLPLDLRMPGAVYCDDADCAAQNLLSPAATISAAKGASTGMISP
                     PQPFGTTMAWQGMLGQNTFMEFPVKPFNPGENGGINGQVVYASTRPFDDPTMLLQLSW
                     EPGVANVKVNLYSETVDASGNTKLQLVDTTMSASFDDWAQGFRKDASGNYVKAADGTS
                     YVPNMNCPGQDASSPFFATLKNSKMWLDAADATGNKKPLAYQSQFKCYDGWSQLNQAQ
                     PAPYDGKYSFPSVNTVGADGKPASTNCHMYSDAEPWGCKLNLAASPSDWDYNKPVLVA
                     GKYVVEVVVPAGYELVKEEDKNILLGDVFIAPVTQQFAGFGNVFIMPDQATINANYNQ
                     HNPGNINLTSNLGSVTFPRHEGDTGSIEAYWPCVGAERIVPDLNSLYPQAGQASPFAG
                     AKRRLCDRKEVQLDDQSSSLAKFYIFSSTHIAGHFTGTITNDFASEFDPFSPQFGEKF
                     GPPNLPVGLRDFNGYEVARVYSDQWGIYNGLYYSTWTVNPPNPTGYAPEMSIACMNDP
                     GPILAADGKTLVTDPQYNPAYSNFCYEQPFMPGTTTYMDTPVIPTMSFADGYNLPDAE
                     YPDGTPAIASVSTGSSAFGPWVDASPPVGSVTVGARGQNYTSAPTVSFSGGGGSGAAA
                     TATMRVASIQVTTPGVGYTNLLQPNNGITVSGGGGTGAAAVTASLKVVAVNFTQGVGN
                     CSTGAGTVTRRLTFNTGGAIATATITNGRVTGIVVNDGSGYIVAPTATLTGCPATTLT
                     LTPRMGVGTVQVALPGSGYTSEPTVNFGAPPSGTGNTTASAVGLLSLNAVTVTAGGSG
                     YSTAPTVAFAGGGGSGATATAVLGSGDGAGVTITINALGDKPVQNPNFSGPNSTGAPY
                     NQKTITRHYGFGSNTGGNGTVALVGADGVQHLLTGVTWSDMKITGNVPSGVPLCSASN
                     KSYTNGTGNPNAGARCGQLVITNGDNGKSSIDTVTVTIGGTKPWVVSPGGVTDSNGNA
                     VSDNGAAFGRMGLSPIQLAIDKANPGDLLLVQPGTYKENLIMWKPVRLQGVGAAAVTI
                     NADAHPAGKMDQWRRQINCAFGLDLQGVPNLGNSQSQYDPSGLYSCPASMFQRVDRIP
                     FEAIVGWDASGNGNLAQVLQEPTLMGAYEGAGITVLARGVRIPAGNTDFWGQNATGGA
                     GAFTDGSRYLTGGNVSAAQAAALGYPGTGGNDCAINPTEVDGRDYGTGNFFCNPSRID
                     GVSIINSSQGGGGVFVHGWAQNLEVANTRISGNQGTLAGAINLGNGETPDAFINDGVE
                     CGVVPSVTPCPPIGTNPLGVNVASGAAIPFQFNTGVRIHHNMLYDNASIGDALFSGTP
                     SGAGAITVSAGADNYRIDHNWIAGNLSTGDGGGVQHLGMAFNGKIDHNFILYNQSTNP
                     TLPTNGGGIAIEGANLDRTLNGNECGSTNDQDCPPGLTEGIGPGLVIDANLIVGNSAE
                     SGSGGGLRIQQINGSADLIAFPTTPSAWYDVTVTNNVIANNVAGYDGGGVSLQDALKV
                     NMINNTVASNDSTASAGALFKTLGAIMSNTPPPGCTPTTDPSLPQNPNCLVADAPHGP
                     QPAGLVVSAHTVNLVDALVGVPTNCTSLAANGYTGVGNANDCRKVSKPHMVNNLFWQN
                     RTFSVNVVDQFGNVVTGNSATPTGKGLQSQQNLIALTPQLAQRSTGECSAGANYWDIG
                     LRTDDVNGGMIPAGTRLALINSLYTGDARSTTIVGSPVSANNQTPTATPLVAPFCNGA
                     RMPPEHCTDGGVDNGSPSCKGYNAPAGASETTGLTQVFVFNGIQPTATVDEGHNWLNL
                     SYGPLTLSKPNVGTGTPAEQMLVGGSSGLGTTVGAYSIPSGSAAAGNGTVGTGLPAQV
                     ASDYFGNARTINGTTKLDIGAVALTTTAASVRPMALTFGTVAIGASSTLTVTLQAGNA
                     PVTGLNVSTAAPFTASAAGCPATIAAHGSCTVTVTFTPVAPGGAASGSLAVTAGAGGP
                     FLGAPVALTGNGQAIAVNATPATVVFPDTVEAQSNVQTVTLTNLSGSAVTGLAGTVNG
                     TGYSLSGNNCPASLAAGNTCTMSVTFAPMQIGSSSGTLTVAATPAVSTSGPVNLVGNG
                     LPAVPVLTTTSPALAIQGSVVPVTLTGQWFTGATQVNVSGTGVTVSNVAVVNDTTITA
                     TFTIARNATVGGRTVTVVTGGGTSNTSAFTVQAPPVVFTAATPPGTLNTATGTLAFGS
                     QNGTVSSTVTVKVNGSAPITFQTAGLAAGSNASYTLGTDTCSGTTVAPGGTCKVTVTF
                     AGAGFANRTADLQLPYTGDLGSPLSLTVTGR"
     gene            8410..8997
                     /locus_tag="VIN75_02985"
     CDS             8410..8997
                     /locus_tag="VIN75_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973156.1"
                     /translation="MAHGPWDGVMPGGRINRPRGRQGGDASIAPATMDRRLPAAVARL
                     RRVTPGRRHAPRLAALALALAVLVPADSSAGATESDAAPDAHARTVAATSLDGRVKLL
                     AAELQLTPEQQLKVRALLEQQREQVRRLWSDTTLAPALRVGRTQGISDRTVEAIRGVL
                     DESQRRRYIQPRQREAAVGTSGADVQSWMQTETKP"
     gene            8994..9251
                     /locus_tag="VIN75_02990"
     CDS             8994..9251
                     /locus_tag="VIN75_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973157.1"
                     /translation="MIRSAWAGACMGVLLSVCGPGAHAASLPATAAEAASAASAPQVR
                     RGSPYARAARQHAMEASATATPKVNPLIQHRPRLPASMRHG"
     gene            9308..9886
                     /locus_tag="VIN75_02995"
     CDS             9308..9886
                     /locus_tag="VIN75_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973158.1"
                     /translation="MVEAKNPCTPRPRPARVDRLAWRGAIAVILAGMCCLAAAQSPGA
                     KAAAPAAAPAASPAAGRFRPVSLSVSRRQQVRSEHAVTYYSAAWGVQDMAVRYTASGN
                     LIRFSYKVVDPARAKALFDKGSQPVLFSQRSHALLQIPVMDKVGPLRQANDLAPGKEY
                     WMAFSNKGNLVRPGDRVNVIIGAFHADGLLVE"
     gene            9923..>10674
                     /locus_tag="VIN75_03000"
     CDS             9923..>10674
                     /locus_tag="VIN75_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY8973159.1"
                     /translation="MRCNQVMPLAALAALLASSIAMAAGLTAEQIADKNAAARGGLAA
                     WRAVDSMKLTGQMDAGGTPAVRLPFEMSLKRPHKSRLELHVKDQVAVQVYDGQQGWKQ
                     RPFLNRDDVEAFTPQETQAAADDADLDGPLIDHARKGIRVELAGTETIEGRKAYRLHL
                     TRADGRKSDLWIDTTTFLDVKMDGEPRRLDNKVHKVSVFYRDYKTVDGLKIPMTLETV
                     VDGVRQSRKISFQGVTVNPALSEALFAKPQPAA"
BASE COUNT         1835 a         3859 c         3588 g         1392 t
ORIGIN      
        1 gcacctggat cccaacaagc gctacttcat ctcgatcctg cccggcgaca gcgtcaatcc
       61 gacgatcggc ggccccggct cgcagccggt ggatccggcc aatccggcgg ggccgcagcg
      121 tccgttcgac atcggcaagg actgcggcgc ctatcccggc ccgaccaccg acggcccttg
      181 ggcgccggcc ggcggcagcg ccatgtgcgg ccacggcatg accggtgcgc agatctccag
      241 ggaactgctc gccgcggcca acccttcgct gacgatcaag gcgcaggaga cgcccctgcc
      301 gacggccaag atctcggtgc tggtgtacca ggacgacaac ccgctcaacg gcgagaacga
      361 cgccggcggc ggcgtcgacg tcatcgcccc gaacgaggca ggcctgggcg gcttcgagat
      421 caagctgttc gaccaggccg gccagctcgg cgacaacacg ggccagatca cgtacgacga
      481 gttcaaccag cccgtgagca actcgctggc caacacgatc gacccgatca ccaagctgga
      541 cgcctgcccg atctcggcgc gcggcgacgg cctggtcggc atgatcccga cgtgcccgaa
      601 gtacgagtcc gacggcaaga cgctgtcgcc gctcgcgggc caggcggtca tcgccaactt
      661 gtaccccggc ctgtatgaga tccaggccta ccccgccgcc gaccgcatcg gccgcggcga
      721 ggaatgggtg cagaccaaca cgctggacgg cggcaagccg cacgaggcct tcatcaagcc
      781 gaacgaaccg ggctacttcc aggagttcgg ccccggcggc ttccacgtga cgatcggctt
      841 cgccaacccg gccatcatca acaaccggct ggcggcctac tgcgcttcgc cggccaacgg
      901 cggcggcacg ggcgcccagg cgtgcgacaa gaagctgacg gtgcatgtcg ccaacagcca
      961 catgagccgc tcgcccgacc agcgcatgta cagcagcggc agctacgaca actactcgtt
     1021 cacgaactgc tacgtctcga tcgggccggc cgacgcggcc gatttcgcct tcgccaagtg
     1081 cgacgccaac ggcaccgcgg tgttcaacaa catcccgaac ggcacgtaca agatctcggt
     1141 gttcgaccag tggaacgaca tcatgctcga cgggctcgtc gggacggtgg cggtcgccgg
     1201 cgacacggaa aaggagttcc ccgtcaccca gtggcgcacg aacctgtaca cgcgcacgtt
     1261 catcgactcc aacggcgacg gcgtctcgca ggatcccggc gagctgggcc tggcgctcgt
     1321 caacaccaac atccgctatc gcgacggcag cttcgcgttc ttcaacaaca ccgacctgaa
     1381 cggctatgcg gcgtggaacg aggtgttccc gttcatgaac tggctcgtgg tcgagacctc
     1441 ggcgacgcgc ttcaagacgc tgggcgtgca ctcggtgtac gacgcgggcg gcccggtgga
     1501 ctgcgacgtg cagtcgacca tctgctccaa catcgccgac cacctggcca acaccgacgt
     1561 gcgcgttccg ctgccgctcg acctgcgcat gcccggcgcc gtctattgcg acgacgccga
     1621 ctgcgcggcg cagaacctgc tgtcgccggc cgccacgatc tccgctgcca agggcgcgtc
     1681 caccggcatg atctcgccgc cgcaaccctt cggcacgacc atggcgtggc agggcatgct
     1741 gggccagaac accttcatgg agttcccggt caagcccttc aatccgggcg agaacggcgg
     1801 catcaacggc caggtcgtct atgcctcgac ccgtccgttc gatgacccga cgatgctgct
     1861 gcaactgagc tgggagccgg gcgtcgccaa cgtcaaggtc aacctgtaca gcgagaccgt
     1921 cgatgcctcc ggcaacacga agctgcagct cgtggacacc acgatgtcgg cgagcttcga
     1981 cgactgggcg cagggcttcc gcaaggacgc cagcggcaac tacgtcaagg ccgcggacgg
     2041 cacgagctac gtgccgaaca tgaactgccc gggccaagat gcctcgagcc cgttcttcgc
     2101 gacgctcaag aacagcaaga tgtggctgga cgcggccgac gcgaccggca acaagaagcc
     2161 gctggcctac cagtcgcagt tcaagtgcta cgacggctgg tcgcagctga accaggcgca
     2221 gccggcgccc tatgacggca agtactcgtt cccgagcgtg aacacggtgg gcgccgacgg
     2281 caagcccgcg agcaccaact gccacatgta ttcggatgcc gagccgtggg gctgcaagct
     2341 caacctggcc gcgtcgccca gcgactggga ctacaacaag cccgtgctcg tcgccggcaa
     2401 gtacgtggtc gaggtcgtgg tgccggccgg ctacgagctg gtcaaggaag aggacaagaa
     2461 catcctgctg ggcgacgtgt tcatcgcgcc ggtcacgcag cagttcgccg gcttcggcaa
     2521 cgtcttcatc atgcccgacc aggcgaccat caacgccaac tacaaccagc acaatccggg
     2581 caacatcaac ctcacgagca acctcggttc cgtgaccttc ccgcgccacg agggcgacac
     2641 cggcagcatc gaggcctact ggccgtgcgt cggcgccgag cgcatcgtgc cggacctgaa
     2701 ctcgctctat ccgcaagccg gccaggcctc gccgttcgca ggcgccaagc gccgcctgtg
     2761 cgaccgcaag gaagtgcagc tggacgacca gtcgtcctcg ctggccaagt tctacatctt
     2821 cagctcgacc cacatcgccg gccacttcac gggcacgatc accaacgact tcgcgtcgga
     2881 gttcgacccg ttctcgccgc agttcggcga gaagttcggc ccgccgaacc tgcccgtggg
     2941 cctgcgtgac ttcaacggct acgaggtcgc ccgcgtctat tccgaccagt ggggcatcta
     3001 caacggcctg tactactcga cgtggacggt gaatccgccc aaccccaccg ggtatgcacc
     3061 cgagatgtcg atcgcctgca tgaacgaccc gggcccgatc ctggccgcgg acggcaagac
     3121 gctggtcacc gacccgcaat acaacccggc gtacagcaac ttctgctacg agcagccgtt
     3181 catgcccggc acgacgacgt acatggacac tccggtgatc ccgacgatgt cgttcgccga
     3241 cggctacaac ctgcccgacg ccgaatatcc tgacggcacg cccgccatcg ccagcgtgag
     3301 caccggttcc agcgcgttcg gcccctgggt cgatgcgtcc ccgccggtgg gatcggtcac
     3361 ggtcggcgcg cgcggccaga actacacctc cgcgccaacg gtgagcttct cgggcggcgg
     3421 cggcagcggc gccgcggcca cggcgacgat gcgcgtggcc tccatccagg tgaccacccc
     3481 gggcgtgggc tacacgaacc tgctgcagcc caacaacggc atcaccgtct ccggcggcgg
     3541 cggcaccggc gccgcggcgg tcacggccag cctgaaggtg gtggcggtca acttcaccca
     3601 gggggtgggg aactgcagca ccggagcggg cacggtgacc cgtcggctca ccttcaacac
     3661 cggcggcgcc atcgcgaccg cgacgatcac caacggccgc gtcacgggca tcgtcgtcaa
     3721 cgacggcagc ggctacatcg tggcgccgac ggcgaccctg acgggttgcc ccgccacgac
     3781 cctgaccctg accccgcgga tgggcgtggg cacggtgcag gtcgcgctgc ctggctccgg
     3841 ctacacgagc gagccgaccg tgaacttcgg tgcgccgccc agcggcacgg gcaacaccac
     3901 ggcgagcgcc gtgggcctgc tcagcctcaa cgccgtcacg gtgacggcgg gcggctcggg
     3961 ctactccacg gcgccgaccg tggccttcgc gggcggcggc ggcagcggtg ccaccgcgac
     4021 cgcggtgctc ggctccggcg acggcgcggg cgtgacgatc acgatcaatg ccctcggcga
     4081 caagccggtg cagaacccga acttctcggg cccgaattcc acgggcgcgc cgtacaacca
     4141 gaagacgatc acgcgccact acggcttcgg cagcaacacg ggcggcaacg gcacggtggc
     4201 cctcgtgggc gccgacggcg tgcagcacct gctgaccggc gtcacgtgga gcgacatgaa
     4261 gatcaccggc aacgtgccga gtggcgtgcc gctgtgcagt gcttccaaca agagctacac
     4321 gaacggcacg ggcaacccca acgccggcgc gcgctgcggc cagctggtca tcaccaacgg
     4381 cgacaacggc aagtcgtcga tcgacaccgt caccgtgacg atcggcggca ccaagccgtg
     4441 ggtcgtctcg cccggcggcg tgaccgactc caacggcaac gccgtgtccg acaacggcgc
     4501 cgcgttcggc cgcatgggcc tgagtcccat ccagcttgcc atcgacaagg cgaacccggg
     4561 tgacctgctg ctcgtccagc cgggcaccta caaggaaaac ctgatcatgt ggaagccggt
     4621 gcgcctgcag ggcgtgggcg ccgcggcggt gacgatcaat gccgacgcgc atccggccgg
     4681 caagatggac caatggcggc gccagatcaa ctgcgcgttc ggcctcgacc tgcagggcgt
     4741 gcccaacctg ggcaacagcc agtcgcagta cgacccgagc ggcctgtact cgtgcccggc
     4801 cagcatgttc cagcgcgtcg atcgcatccc gttcgaggcg atcgtgggct gggacgcgtc
     4861 cggcaacggc aacctggcgc aggtgctgca ggagcccacg ctgatgggcg cgtatgaagg
     4921 cgcgggcatc acggtgctcg cccgtggcgt gcgcatcccg gccggcaaca cggacttctg
     4981 gggccagaac gccaccggcg gcgcgggcgc cttcaccgac ggcagccgct acctgacggg
     5041 cggcaacgtg agcgccgcgc aggccgctgc cctcggctac ccgggcaccg gcggcaacga
     5101 ctgcgcgatc aacccgacgg aggtcgacgg tcgcgattac ggcaccggca atttcttctg
     5161 caacccgtcg cgcatcgacg gcgtgtccat catcaacagc tcgcaaggcg gtggcggcgt
     5221 gttcgtgcac ggctgggccc agaacctcga ggtcgccaac acgcgcatct cgggcaacca
     5281 gggcacgctg gcaggcgcga tcaacctggg caacggcgag acgccggacg ccttcatcaa
     5341 cgacggcgtc gagtgcggcg tggtgccgtc cgtgaccccg tgcccgccga tcggcaccaa
     5401 cccgctgggc gtgaacgtgg ccagcggcgc ggcgatcccg ttccagttca acaccggcgt
     5461 gcgcatccac cacaacatgc tgtatgacaa cgcatcgatc ggcgacgcgc tgttctcggg
     5521 cacgccctcg ggcgcaggag cgatcaccgt cagtgccggc gcggacaact accgcatcga
     5581 ccacaactgg atcgcgggca acctgtcgac cggcgacggc ggcggtgtcc agcacctggg
     5641 catggccttc aacggcaaga tcgaccacaa cttcatcctc tacaaccaga gcaccaaccc
     5701 gaccctgccg accaacggcg gcggcatcgc gatcgagggc gccaacctcg accgcaccct
     5761 caacggcaac gagtgcggca gcaccaacga ccaggactgc ccgccgggac tgacggaagg
     5821 catcggcccg ggcctggtga tcgacgccaa cctcatcgtg ggcaacagcg ccgagagcgg
     5881 cagcggcggc ggcctgcgca tccagcagat caacggctcg gccgacctga tcgcgttccc
     5941 gacgactccg tcggcctggt acgacgtcac ggtgaccaac aacgtcatcg cgaacaacgt
     6001 ggcgggctat gacggcggcg gcgtgtcgct gcaggacgcc ttgaaggtca acatgatcaa
     6061 caacacggtg gcgtccaacg acagcaccgc ctcggccggg gcgctgttca agacgctggg
     6121 cgcgatcatg tcgaacacgc cgcccccggg ctgcacgccg acgaccgatc cgagcctgcc
     6181 gcagaacccg aactgcctgg tggccgacgc gccgcacggc ccgcaacctg ctggcctggt
     6241 ggtgtccgcc cacacggtga acctggtgga cgcgctcgtg ggcgtgccga cgaactgcac
     6301 gagcctggcc gccaacggct acacgggcgt gggcaacgcg aacgactgcc gcaaggtgtc
     6361 caagccgcac atggtcaaca acctgttctg gcagaaccgc acgttcagcg tgaacgtggt
     6421 cgaccagttc ggcaacgtcg tgacgggcaa ctcggccacg cccacgggca agggcctgca
     6481 gtcgcagcag aacctgatcg cgctgacgcc gcaactggca cagcgttcca cgggtgaatg
     6541 ctcggcgggc gcgaactact gggacatcgg cctgcgcacc gacgacgtca acggcgggat
     6601 gatcccggcc ggcacccgcc tggccctgat caactcgctg tacaccggcg atgcgaggtc
     6661 gacgacgatc gtgggcagcc ccgtgagcgc caacaaccag acgccgaccg ccacgccgct
     6721 ggtggctccg ttctgcaacg gcgcgcgcat gccgcccgag cactgcaccg acggcggcgt
     6781 cgacaacggc agcccgtcct gcaagggcta caacgccccg gccggtgcgt cggagacgac
     6841 gggcctgacc caggtcttcg tgttcaacgg catccagccg acggccacgg tggacgaagg
     6901 ccacaactgg ctgaacctgt cgtacggccc gctcacgctg tccaagccca acgtcggcac
     6961 cggcacaccg gccgagcaga tgctcgtcgg cggctccagc ggcctgggca cgacggtggg
     7021 ggcgtactcg atcccgagcg gctccgccgc ggcgggcaac ggcacggtcg gcaccggcct
     7081 gcccgcgcag gtcgcgagcg actacttcgg caacgcgcgc accatcaacg gcaccaccaa
     7141 gctggacatc ggcgcggtgg ccctgacgac gaccgcggcc tcggtgcggc cgatggcgct
     7201 gaccttcggg acggtggcga tcggcgcgtc gagcacgctg acggtgacgc tgcaagccgg
     7261 caacgcgccg gtgaccggcc tgaacgtcag cacggcggca ccgttcacgg cctcggccgc
     7321 gggctgcccc gccacgatcg ccgcgcacgg ctcctgcacg gtcacggtca ccttcacgcc
     7381 ggtcgcgccg ggcggcgcgg cctccggctc gctggcggtc acggccggcg cgggtgggcc
     7441 gttcctgggc gcgccggtgg ccctcacggg caacggccag gccatcgcgg tgaacgcgac
     7501 gccggcgacg gtcgtgttcc ccgacacggt cgaggcccaa agcaacgtgc agaccgtcac
     7561 gctgacgaac ctgtccggtt ccgcggtgac cggcctcgcg ggcacggtga acggcacggg
     7621 ctactcgctg agcggcaaca actgcccggc gtcgctcgcg gccggcaaca cctgcacgat
     7681 gagcgtcacg ttcgcgccga tgcagatcgg ctcgagcagc ggcacgctga cggtggctgc
     7741 caccccggcg gtgtcgacgt cgggcccggt caacctggtc ggcaacggcc tgcccgcggt
     7801 gccggtcctg acgacgacca gcccggcgct cgccatccag ggcagcgtcg tgccggtcac
     7861 gctgactggc cagtggttca ccggggcgac ccaggtgaac gtcagcggca ccggcgtgac
     7921 ggtcagcaac gtcgcggtgg tcaacgacac cacgatcacc gcgacgttca cgatcgcccg
     7981 caatgcgacg gtcggcggcc gcaccgtgac tgtcgtgacc ggcggcggca ccagcaacac
     8041 gagcgccttc acggtgcagg cgccgccggt ggtcttcacc gcggcgaccc cgccgggtac
     8101 gctgaacacc gcgaccggga ccctggcgtt cggaagccag aacgggacgg tgtccagcac
     8161 ggtcacggtc aaggtgaacg gcagcgcgcc catcacgttc cagacggcgg gcctggcggc
     8221 cggcagcaac gcgtcgtaca ccctgggcac ggacacgtgc tcgggcacga ccgtggcccc
     8281 gggcggcacc tgcaaggtga ccgtgacgtt cgcgggcgcg ggcttcgcca accgcacggc
     8341 ggacctgcag ttgccctaca ccggtgacct gggcagtccg ctgtccctga cggtgacggg
     8401 acgctgacca tggcgcacgg cccctgggac ggcgtgatgc ccggcggacg gatcaaccgt
     8461 ccgcgcgggc ggcagggcgg ggacgcgtcc atcgcgcccg cgacgatgga caggcggctg
     8521 ccggcagcgg tcgcccgcct ccggcgcgtc acgcccgggc ggcgccacgc gccgcgcctg
     8581 gcggccctgg cactggcgct cgcggtgctc gtcccggcgg attcgtcggc cggcgccacc
     8641 gagtccgatg ccgcgccgga cgcgcacgcc cgcacggtgg ccgcgacctc cctcgacgga
     8701 cgcgtcaagc tgctggccgc cgaactccag ctgacgccgg agcagcagct gaaggtgcgc
     8761 gccctgctgg agcagcagcg cgagcaagtg cggcgcctgt ggagcgacac gacgctcgcg
     8821 ccagcgttgc gcgtgggccg cacgcagggg atcagcgatc gcacggtgga ggcgatccgc
     8881 ggcgtgctcg acgagtcgca gcgtcgccgc tacatccagc cgcggcagcg cgaggccgcc
     8941 gtgggaacct cgggggccga cgtgcagtca tggatgcaga cggagaccaa gccatgatcc
     9001 ggtccgcctg ggcaggcgcc tgcatgggcg tcctgttgtc ggtttgcggg ccgggcgcgc
     9061 acgccgcctc gttgccggcc actgcggccg aggccgcgtc ggcggcgtcg gcgccgcagg
     9121 tccggcgcgg gtcgccctac gcgcgcgccg cgcgccagca cgcgatggag gcctccgcga
     9181 ccgcgacgcc gaaggtcaat ccgctgatcc agcaccggcc gcgcctgccc gcgtccatgc
     9241 gacacgggtg atgcggatcg gaagtttccg ataggcagag gccgcgtgtt ccactagtgt
     9301 tggtcccatg gtcgaagcga agaacccttg cacgccccgc ccgcgtcctg cccgcgtcga
     9361 ccggctggcc tggcgcggtg cgatcgccgt gatcctggca ggcatgtgct gcctcgctgc
     9421 cgcccaatcg cccggcgcga aggccgcggc gcccgcggcg gccccggccg cgtcgccggc
     9481 ggcggggcgc ttccgcccgg tcagcctgtc ggtctcgcgc cggcagcagg tgcgcagcga
     9541 gcacgccgtc acctactaca gcgcggcgtg gggcgtgcag gacatggccg tgcgctacac
     9601 cgcctccggc aacctgatcc ggttcagcta caaggtggtc gacccggcgc gggccaaggc
     9661 gctgttcgac aagggcagcc agcccgtgct gttctcgcag cgcagccacg cgctgctgca
     9721 gatcccggtg atggacaagg tgggcccgct gcggcaggcc aacgacctgg cgccgggcaa
     9781 ggagtactgg atggcgttct ccaacaaggg caacctcgtg cgcccgggag accgcgtgaa
     9841 cgtgatcatc ggcgcgttcc acgccgatgg cctgctggtg gagtgaacgc gcgccgcggc
     9901 gtgcacaagg ggagggcgga tcgtgagatg caaccaagtc atgccgctgg cggcgcttgc
     9961 ggccttgttg gcgagctcga tcgccatggc cgcgggcctc acggccgagc agatcgcaga
    10021 caagaatgcg gccgcccgcg gcggcctggc cgcctggcgg gcggtcgact cgatgaagct
    10081 gaccggccag atggacgccg gcggcacccc ggccgtgcgc ctgccgttcg agatgagcct
    10141 gaagcgcccg cacaagagcc ggctcgaatt gcacgtcaag gaccaggtcg ccgtccaggt
    10201 ctatgacggc cagcagggct ggaagcagcg gcccttcctg aaccgcgacg acgtcgaggc
    10261 cttcacgccg caggagaccc aggccgccgc cgacgatgcc gatctcgacg gcccgctgat
    10321 cgaccacgcg cgcaagggca tccgggtcga gctggccggc accgagacga tcgagggccg
    10381 caaggcctac cgcctgcact tgacgcgcgc ggacggccgc aagtccgacc tgtggatcga
    10441 cacgacgacc ttcctggacg tcaagatgga cggcgagccg cggcgcctgg acaacaaggt
    10501 gcacaaggtc agcgtgttct accgcgacta caagacggtg gacggcctga agatcccgat
    10561 gacgctggag acggtcgtcg acggggtgcg gcagtcccgc aagatcagct tccagggcgt
    10621 gacggtgaat cccgcgctgt cggaggcgct gttcgccaag ccgcagcctg ccgc
//
LOCUS       DATCRO010000110         7040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1103852, whole genome shotgun sequence.
ACCESSION   DATCRO010000110 DATCRO010000000
VERSION     DATCRO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103852"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VIN75_03005"
     CDS             complement(<1..648)
                     /locus_tag="VIN75_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973160.1"
                     /translation="MSRPSVIPFPAPLRRLRAQLAPLVLPGKERSGPNSMFDSAGALG
                     VTVEVPESEQGALALHAHLASSPEVSSMLVIDPSGVWAHGMMQTLSDATGSPVDRLRV
                     HSRGGLQIRAVVDETLLPAGNGPHRLVRCLHGDAKPVDRRAIFGALLSHSQVCSVLVG
                     PMPSHEAVALLEEMHRLALQPFASKTRWLFYLAAEHRGLEDLIEAREWPIRPTLLH"
     gene            847..2565
                     /locus_tag="VIN75_03010"
     CDS             847..2565
                     /locus_tag="VIN75_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8973161.1"
                     /translation="MPSVSARLAGHALVEALIEQGVDTVFGVPGESFLAALDGFHQHA
                     DRIRFIACRHEGGAAFMAEAHGKLTGRPGVCFVTRGPGATNASIGVHVAFQDSTPMVL
                     LVGQVAADQRDREAFQEIDYRQMFGPGTLGLAKWVGEVHSADRLPEYLARAFRTAMQG
                     RPGPVVLALPEDMLSTLTCAPVLARVEPARAWPDPHVLEQFRQLLIRSERPFAIVGGS
                     GWTAAAAADFERFVEDWEIPVGCAFRYQDVFDNRHRLYAGDVGIGINPKLAQRIRESD
                     LLLAIGVRMGEMTTSGYTLPEAPVPRQPLVHIHAGAEELGRVYQPTIALQCSMGAIGD
                     ALEDIDPPTPRSPVEGALAHAEYLANLEAARVEPMDLAVVVKTIEAMAPAGTVYTNGA
                     GNYAGWLHRFHRYTSLQREGRTQLAPTAGAMGYGVPAAIAANVLFPDRTAVAIAGDGD
                     FLMTGQELATATAHAAGRGAGKLVVVVVDNGTYGTIRMHQERDYPARVSGTTLTNPDF
                     VQLARAYGWHAERVETTAAFEPAFGRAMQAHGPTLLHLLLPEDVSTSRTTLTALRAAA
                     LKRQGA"
     gene            2850..3071
                     /locus_tag="VIN75_03015"
     CDS             2850..3071
                     /locus_tag="VIN75_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY8973162.1"
                     /translation="MDPIVFELRGDHITLDALLKAAGLASSGGDAKTLIQQGGVLVNG
                     EVDLRRGRKLRAGDEVAVGGRKVELRAGA"
     gene            3192..3956
                     /locus_tag="VIN75_03020"
     CDS             3192..3956
                     /locus_tag="VIN75_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973163.1"
                     /translation="MNPALVRTRDRPAASRRSLRALLFVLLAVPLPPAAGALDLSQKL
                     AWPAPIDDGVLEVGDRRLHLPPGHWVLTGRREVDTVGQARGGDAWGRGVSAWATLVEN
                     GHLRAIVWLGLPLQDLRNVREGRHGCADNDATIERLNLSSQLSKPECLAVYGERNLQS
                     ALGTRTPHTLNWLSKRHVADPGPLVRFVYKLRSESSYGSFMLVLPTGPFDSDEEARHW
                     ALGLRESLRPFLEHRTSEATVPALPGATSSGAASVR"
     gene            4426..5696
                     /locus_tag="VIN75_03025"
                     /pseudo
     CDS             4426..5696
                     /locus_tag="VIN75_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349365.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            5722..6057
                     /locus_tag="VIN75_03030"
     CDS             5722..6057
                     /locus_tag="VIN75_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973164.1"
                     /translation="MLEMECFAIAARLHVVMRRIVGRVTDVDWMVRNREYAREVVRVA
                     RLQTDAELLALADKFETALDQVRAPDATTRRANEAARAAADAREGAAAAAPVTHEIPA
                     IPRYVGRLR"
     gene            complement(6180..6256)
                     /locus_tag="VIN75_03035"
     tRNA            complement(6180..6256)
                     /locus_tag="VIN75_03035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6220..6222),aa:Pro,seq:tgg)
     gene            complement(6269..>7040)
                     /locus_tag="VIN75_03040"
     CDS             complement(6269..>7040)
                     /locus_tag="VIN75_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HEY8973165.1"
                     /translation="PDEDGLFDCGRPDVPAAGRLRIAVLCLPHISNLDEFEPLRRMPG
                     VQLAWARAEAELHGADWIVLPGSKQVSGDLAWLRRQGLDRAIARHAADGRPVLGICGG
                     LQMLGEALVDPEGYDGEAGFNGPGLGLLPLVTRYERDKRVRRTRLRFERTTGAWAPWA
                     GVEAAGYEIHCGVTVPHPGLAAPTVALRDAHGEAIGWQHGSVLGVYAHGLFESPSLLR
                     AMWGAGAPALDDSFELLADLVDRHLDATLLDRLLSPSR"
BASE COUNT          944 a         2471 c         2573 g         1052 t
ORIGIN      
        1 atgcagcagc gtcgggcgga tcggccattc gcgcgcctcg atcaggtctt ccaggccgcg
       61 gtgctccgcg gcgaggtaga acagccagcg cgtcttggac gcgaacggct gcagcgcgag
      121 gcggtgcatc tcttccagca gcgcgacggc ctcgtggctg ggcatcggcc cgaccagcac
      181 cgagcagacc tgcgaatgcg acagcaaggc gccgaagatg gcgcggcggt cgacgggctt
      241 ggcgtcgccg tgcaggcagc gcaccaggcg gtgcgggccg ttgccggcgg gcaacagggt
      301 ttcgtcgacc acggcgcgga tctgcaggcc gccgcgcgag tgcacgcgca ggcggtcgac
      361 cggcgagccg gtggcgtccg acagcgtctg catcatgccg tgggcccaga cgcccgacgg
      421 gtcgatgacc agcatggagc tgacctcggg cgaggacgcc aggtgggcgt gcagcgccag
      481 cgcgccttgc tcgctttcgg gcacttccac cgtgacgccc agggcgccgg cggagtcgaa
      541 catcgagttg gggcccgacc gctccttgcc cggcagcacc agcggcgcga gctgcgcccg
      601 cagcctgcgc agcggggcgg ggaagggaat gacggaggga cgcgacatga agttgagctt
      661 actcggacag gtcgaaatga ccggcgtcca aatgtttacg gatgtgactt ccttatcgcg
      721 aactggattt tcatacatgc ctcctacgcc cgtacaggaa cggtcgttca acgcgcgcga
      781 gtcggttgca cgggcttgca cccggctacc atcgacactt tgtctcggac cttcccggtg
      841 agttccatgc cctccgtatc cgcccggctt gccggccacg ccctcgtcga ggccctgatc
      901 gaacagggcg tcgacaccgt cttcggcgtc cccggcgaga gcttcctggc cgcgctcgac
      961 ggcttccacc agcacgccga ccgcatccgc ttcatcgcct gccgccacga gggcggggcc
     1021 gcgttcatgg ccgaggccca cggcaagctc acgggccggc cgggcgtgtg cttcgtcacg
     1081 cgcggcccgg gggccaccaa cgccagcatc ggcgtgcacg tcgccttcca ggactcgacg
     1141 cccatggtgc tgctggtggg ccaggtcgcc gccgaccagc gcgatcgcga ggccttccag
     1201 gagatcgact accgccagat gttcgggccc ggcacgctgg ggctggccaa gtgggtcggc
     1261 gaggtgcact cggccgaccg cctgcccgag tacctcgcgc gcgccttccg caccgcgatg
     1321 cagggccggc cgggtccggt ggtgctggcg ctgccggagg acatgctgtc gacgctgacc
     1381 tgcgcccccg tgctggcgcg cgtggagccc gcgcgcgcct ggccggaccc gcatgtcctc
     1441 gagcagttcc gccagttgct gatcaggtcc gagcggccgt tcgcgatcgt cggcggcagt
     1501 ggctggacgg ccgccgcggc ggccgacttc gagcgcttcg tcgaggactg ggagatcccg
     1561 gtcggctgcg cgttccgcta ccaggacgtg ttcgacaacc ggcaccggct gtacgcgggc
     1621 gacgtcggca tcgggatcaa tcccaagctt gcacagcgca tccgggagtc ggacctgctg
     1681 ctcgcgatcg gtgtccgaat gggcgaaatg accacgagcg gttacacatt gcccgaggcg
     1741 ccggtgccgc gccagccgct ggtgcacatc cacgccggcg cggaggagct gggccgggtc
     1801 taccagccca ccatcgcctt gcaatgctcg atgggcgcca ttggcgacgc gctggaggac
     1861 atcgacccgc cgacgccgcg ctcgcccgtc gagggcgccc tggcgcacgc cgagtacctg
     1921 gccaacctgg aggccgcgcg ggtcgagccg atggacctcg ccgtcgtcgt caagacgatc
     1981 gaggcgatgg cccccgccgg caccgtgtac acgaacggcg caggcaacta tgcgggttgg
     2041 ctgcaccgct tccaccgtta caccagcctg cagcgcgagg gccgcacgca gctggcgccg
     2101 accgccggcg cgatgggcta cggcgtgccg gccgcgatcg cggccaatgt cctgttcccc
     2161 gaccgcacgg ccgtggcgat cgccggcgac ggcgacttcc tgatgaccgg ccaggagctg
     2221 gccacggcca cggcgcacgc ggccgggcgg ggcgccggca agctggtggt cgtggtggtg
     2281 gacaacggca cctacggcac gatccgcatg caccaggagc gcgactaccc ggcgcgcgtc
     2341 agtggcacga cgctcaccaa cccggacttc gtgcagctcg cgcgcgccta cggctggcac
     2401 gccgagcgcg tggagacgac ggccgcgttc gagccggcct tcggccgcgc gatgcaggcc
     2461 catggcccca cgctgctgca cctgctgctg cccgaggacg tgagcacgtc ccgcacgacg
     2521 ctcaccgcgt tgcgcgcggc cgcgctgaag aggcagggcg cctaggagtc tgcgcgacag
     2581 atacctgctg cgaacggtaa ttctgccgcg cagactcccg gctctaccgc gaacgtggta
     2641 tcgaacgggc acgctggtgc gccgttgtcc tacacgcacg gcgcgccgca tcgccgatag
     2701 tgaggtctcc cggcggcgca cctcctccct ccctcctctt tggtcgccgg tctcagcccg
     2761 gccctgtgcc gggctctttt ctttgcgcgg cggaaaaacg cggtacgctg gcggccctcc
     2821 aacgcctgcc tgcagcgaaa gccttcccaa tggatcccat cgtgttcgag ctgcgcggcg
     2881 accacatcac cctcgacgcg ctgctgaagg cggccgggct ggccagcagc ggcggcgacg
     2941 cgaagacgct gatccagcag ggcggcgtgc tcgtcaatgg cgaggtcgac ctgcggcgcg
     3001 gccgcaagct gcgcgccggc gacgaggtgg ccgtgggcgg gcgcaaggtc gagctgcgcg
     3061 cgggcgcgtg agctgggcgt ccggcggcgt ccgacgcatc ggctgatcag tcctgcggga
     3121 ttgacgcggc gccggacggc gcctgaaatc ggcccgcggt cgcgccgtcc cgcgcgtccc
     3181 cggaggggcc catgaatccc gcactcgtcc gaacgcgcga tcggccggca gcgtcccggc
     3241 gatcgctgcg tgcgttgctg ttcgtgctgc tcgccgtgcc gctgccgccg gcggccggcg
     3301 cgctcgacct gtcgcagaag ctggcctggc ctgcgccgat cgacgacggc gtgctcgaag
     3361 tgggcgaccg tcgcctgcac ctgccgccgg gccattgggt gctgacgggg cgccgcgagg
     3421 tcgacacggt cggccaggcg cgcggcggcg atgcgtgggg ccgcggcgtc agcgcctggg
     3481 ccacgctggt cgagaacggc cacctgcgcg cgatcgtctg gctgggcctg ccgctgcagg
     3541 acctgcgcaa cgtgcgcgag ggcaggcacg gctgcgcgga caacgacgcg accatcgaac
     3601 gcctgaatct ctccagccag ttgtccaagc ccgaatgcct ggccgtgtat ggcgaacgca
     3661 acctgcagtc cgcgctcggg acgcgcacgc cgcacacgct caattggctc tcgaagcggc
     3721 atgtcgccga cccgggcccg ctcgtgcgct tcgtctacaa gctgcgctcc gaatccagct
     3781 acggcagctt catgctcgtg ctgcccacgg gcccgttcga cagcgacgag gaggcacggc
     3841 actgggcgct gggcctgcgc gagtcgctgc gtccgttcct ggagcaccgg acctcggagg
     3901 cgacggtgcc ggcgttgccg ggcgccacgt ccagcggcgc ggcttcggtc cggtagctgc
     3961 gttgtcgtgc agcgcgggtc ggtcgtcgac gtggccgccc tgcagccatt gcgggcagcg
     4021 atggaacagg caacgggcgc cgccgccgtg cacccagtcg gcgccctggc tcgagcggag
     4081 gtcgcaagac ggtgcgccgg tcccggtgcc gtgtcccgtg tgcggtggcg ctggcttctc
     4141 gcttcgtgaa ggccgtttcg tcgcgccgcg aagcgaccac gccctgcggg tgtgccaagc
     4201 gtcacgaacc gcctcatgca gcggcggctt cgtctcttca tcgccacaag acgccgaaca
     4261 atatccggcg gcggcccctc cggtgcgtca aatgtgtgga ttctggcctg cttcttgcgg
     4321 ctgagtagcg aaatccacca acaacaaggg tcgtcgatgg gaattcgctt ttccgggccg
     4381 gggtgcgcgc tgcgtgccgg cctcgtcgcc gcgccggcgg cgttcatgtg cagcgcggcg
     4441 gcgggcgccg aggccatcgc gcggcccacg cgcgtgcact tctcccaggt gagcgcgggc
     4501 gacaccgcgt ggatgatctc ggccagcgcg ctggtgctgc tgatgacggt gccggggctg
     4561 gcgctgttct acgccggcat ggttcgcaag aagaacatgc tggcgacgat ggcgcagtcg
     4621 ttcgccatct gcgcgctggt gtcggtgctg tggttcgtgg ccggctactc gctggccttc
     4681 atgcccggct cgccgtggat gggtgacctg tcgtcgctgt tcatgcagac gatggcctac
     4741 gacaagctcg ccggcatcgt gtcggtgcac cacttggcca ccacgatccc ggagtcggtc
     4801 ttcgcgatgt tccagctgac cttcgcgatc atcacgccgg cgctcatcac cggggcgttc
     4861 gccgagcgca tgcgcttttc cgcgctgctc gtgttcatgg cggcgtgggc gctgctagtc
     4921 tacgcgccca tcgcgcactg ggtgtgggag cccacgggct ggctcgcgtc gcgcggcgtc
     4981 ctcgacttcg caggcgggac cgtcgtgcat gtcaacgcgg gcatcgccgg cctggtggcg
     5041 gcgtggatgc tgggtccgcg gcagggcttc cggcgcgagg ccctgatgcc cgccaacctc
     5101 ggctacacga tgatcggcgc gtcgctgctg tgggtgggct ggatgggctt caacggaggc
     5161 tcggccggcg ccgccgacgg tcgcgccggc atggccatgc tggtgacgca gctggccacc
     5221 ggcgcggccg cgctcgcgtg gatgctggcg gaatggctgg tgcgcggcac gcccacgctg
     5281 ctgggcctgt gcagcggcgc cgtggccggg ctggtggccg tcacgccggc ctcgggcttc
     5341 gtcgatccga aggcggcgct ggccatcggc gccatcgccg gcgtgggctg ctactggggc
     5401 gcgaccggcc tgaagcgcct gctgggcgcg gacgattcgc tcgacgtgtt cggcgtgcac
     5461 ggcgtcggcg gcacgatcgg cgcgctgctc accggcgtgt tcgcggtgcc ggcgatcggc
     5521 ggcgtgacgg gctcggtggc gacccaggcc ctgggcgtcg gcgccacgct ggccacagcg
     5581 gcgcagccac ggcggtgatc ctgtgggcgg tggacaaggc catcggcctg cgcgtggacg
     5641 ccgagcagga actggtcggg ctcgaccagg cgctgcacgg cgaacgcgtg gagtgagatt
     5701 taacttcgtt cggaggtggt catgctggaa atggaatgct ttgccatcgc ggcgcggctg
     5761 cacgtggtga tgcggcgcat cgtcggccgc gtcaccgacg tcgactggat ggtgcgcaat
     5821 cgcgagtacg cgcgcgaggt ggtgcgcgtg gcgcgactgc agaccgatgc cgagctgctg
     5881 gcgctggccg acaagttcga gaccgccctg gaccaggtgc gggcgccgga cgccaccacg
     5941 cgccgcgcga acgaggccgc gcgtgccgcc gccgatgcgc gcgaaggcgc ggccgcggcg
     6001 gcgcccgtca cgcacgagat cccggcgatc ccgcgctacg tcgggcgcct gcgctagggc
     6061 gacgccgacc gcaccggctt cgtctttcga cttgcgcatc ctttccccgt cgcgccatcc
     6121 cggcgttgcc cgggaactgc cgatggaaag gcgatgcgtt cccaagtcgt ggattcagat
     6181 ggtcggagcg acacgattcg aacgtgcgac cctctcgtcc caaacgagat gcgctaccag
     6241 gctgcgctac gctccgaagg gcgggattct acctgctcgg agacagaagc cgatcgagca
     6301 gcgtggcgtc gaggtgtcgg tcgacgaggt cggcgagcag ttcgaagctg tcgtcgagcg
     6361 cgggagcgcc cgcgccccac atcgcgcgca gcagcgatgg cgactcgaac aggccgtgcg
     6421 cgtaaacgcc cagcacgctg ccgtgctgcc agccgatcgc ctcgccgtgc gcgtcgcgca
     6481 gcgcgaccgt gggcgccgcc agcccggggt ggggcacggt gacgccgcag tggatctcgt
     6541 agccggcggc ctcgacgccg gcccagggcg cccaggcgcc ggtcgtgcgc tcgaagcgca
     6601 gtctcgtgcg gcgcacgcgc ttgtcgcgtt cgtagcgcgt gaccagcggc agcaggccca
     6661 ggccggggcc gttgaagccg gcctcgccgt cgtagccttc cgggtcgacc agcgcctcgc
     6721 cgagcatctg caggccgccg cagatgccca gcaccgggcg gccgtcggcg gcgtggcggg
     6781 cgatcgcgcg atcgagtccc tggcggcgca gccaggccag gtcgccgctg acctgcttgg
     6841 agcccggcag cacgatccag tcggcgccgt gcagctcggc ctcggcgcgg gcccacgcga
     6901 gctgcacgcc gggcatgcgg cgcagcggct cgaactcgtc gaggttgctg atgtgcggca
     6961 ggcacagcac cgcgatgcgc agtcggcccg cggcggggac gtcgggacgt ccgcagtcga
     7021 acagcccgtc ctcgtcgggc
//
LOCUS       DATCRO010000111         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1115008, whole genome shotgun sequence.
ACCESSION   DATCRO010000111 DATCRO010000000
VERSION     DATCRO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115008"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VIN75_03045"
     CDS             complement(<1..704)
                     /locus_tag="VIN75_03045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8973166.1"
                     /translation="MGMDDRDGKIWMDGELVDWRDAKIHVLTHTLHYGCGAFEGVRAY
                     KTASGATAIFRLREHTERLFNSAKILRMKIPFTLEQVMDAQRAVVRENKLESCYLRPL
                     TWIGSEKLGVSPKGNKIHLMVAAWAWGAYLGEEGLKRGIRVKTSSYTRHHVNITMTQA
                     KAVSNYSNSILANMEAVDDGYDEALLLDSAGFVSEGAGENIFVIKNGVVYTPDLSAGA
                     LNGITRNTIFAICQDLG"
     gene            799..1341
                     /locus_tag="VIN75_03050"
     CDS             799..1341
                     /locus_tag="VIN75_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY8973167.1"
                     /translation="MPSTPPSSPPPANVQLDHASHRATGTLPLRLLAHDVDLALNVGA
                     LFRIADALGVEHLHLTGTSPRPPDPKIRRSARSAERHVAWSHAADPLAVVAQLKAQGW
                     RVVALELSTRSVALEAFDVAPGDRVCLVLGTENAGVSQALLDAADATVHIPMRGHNSS
                     MNVANACAIAAYEIGGRLGG"
     gene            complement(1391..>2223)
                     /locus_tag="VIN75_03055"
     CDS             complement(1391..>2223)
                     /locus_tag="VIN75_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8973168.1"
                     /translation="SDIHGNLPALEAVWAEIERAQPALVVNLGDIASGPLWPRETVQW
                     LMAREAAEPARWRTIAGNHERQALAPDVARMGASDAFAARALGAVERAWLAALPATHR
                     LFEDVLLCHGTPASDLVYFMETVTPGFGVDGQRGMRAATAVELAARAAEMPPGESRAG
                     LASALILCGHTHVPRTMAVPGGPLVANPGSVGLQAYEDDHPFAHVVENGSPDARWALL
                     ERDRRGAWHVQLRSTPYDWQAAAARAEANGRGDWADALATGFMGRRAGRPQGSPRAPL
                     "
BASE COUNT          293 a          784 c          822 g          324 t
ORIGIN      
        1 cccaggtcct ggcagatcgc gaagatcgtg ttgcgcgtga tgccgttgag cgcgccggcc
       61 gacaggtcgg gcgtgtagac gacgccgttc ttgatgacga agatgttctc gcccgcgccc
      121 tcggagacga agccggccga gtcgagcagc agcgcctcgt cgtagccgtc gtccacggct
      181 tccatgttgg ccaggatcga gttggagtag ttggacaccg ccttggcctg cgtcatcgtg
      241 atgttgacgt ggtggcgcgt gtagctggac gtcttgacgc ggatgccacg cttgaggcct
      301 tcctcgccga ggtaggcgcc ccaggcccag gccgcgacca tcaggtggat cttgttgccc
      361 ttggggctga cgcccagctt ctccgagccg atccaggtga gcgggcgcag gtagcagctc
      421 tcgagcttgt tctcgcgcac gaccgcgcgc tgcgcgtcca tcacctgttc cagcgtgaag
      481 gggatcttca tgcgcaggat cttggccgag ttgaacaggc gctcggtgtg ctcgcgcagg
      541 cggaagatgg cggtggcgcc gctggccgtc ttgtaggcgc gcacgccttc gaaggcgccg
      601 cagccgtagt gcagcgtgtg cgtgaggacg tggatcttgg cgtcgcgcca gtcgacgagc
      661 tcgccgtcca tccagatctt gccgtcgcga tcgtccatgc ccatgggaat gcctcgaaag
      721 aatgggaaaa cccgaattct aggaccccgg cgccggcccg gggcgcgcgg cgcgccatcc
      781 tgcagaattc cgcccgatat gccgtccacg ccgccgtctt ccccgccgcc cgccaacgtc
      841 cagctcgatc acgcgagcca tcgcgccacc gggacgctgc cgctgcgcct gctggcgcac
      901 gacgtcgacc tggcgctcaa cgtcggcgcg ctgttccgca tcgccgacgc gctgggagtc
      961 gagcacctgc acctgaccgg cacgtcgccg cggccgcccg acccgaagat ccgccgcagc
     1021 gcgcgctcgg cggaacgtca cgtcgcgtgg tcgcacgcgg ccgacccgct ggccgtcgtc
     1081 gcgcagctga aagcgcaggg ctggcgcgtc gtggcgctgg agctgagcac gcgcagcgtc
     1141 gcgctggagg cgttcgacgt cgcgccgggc gatcgcgtgt gcctggtgct gggcaccgag
     1201 aacgcgggcg tgagccaggc gctgctggac gcggccgatg ccaccgtgca catcccgatg
     1261 cgggggcaca actcgtcgat gaacgtggcc aatgcgtgtg cgatcgcggc gtacgagatc
     1321 ggggggcgcc tgggcggttg aggcgccgcg tgcccgggcc gcgatcgccc gagcgacggc
     1381 gtgcgccggt tcagaggggg gcgcggggtg acccctgcgg ccgacccgct cggcggccca
     1441 tgaaaccggt ggccagggcg tcggcccagt cgccccggcc gttggcctcg gcgcgggcgg
     1501 cggcggcctg ccagtcgtag ggggtcgagc gcagctgcac atgccacgcg ccgcgccggt
     1561 cgcgctccag cagcgcccag cgtgcgtccg gcgagccgtt ctcgacgacg tgcgcgaacg
     1621 ggtggtcgtc ctcgtaggcc tgcagtccca cgctgcccgg gttggccacg agcggcccgc
     1681 ccggcaccgc catcgtgcgc ggcacgtggg tgtggccgca caggatcagc gccgacgcca
     1741 gccccgcgcg ggactcgccg ggcggcatct cggcggcgcg cgcggccagt tcgacggccg
     1801 tggccgcgcg catgccgcgc tggccatcga cgccgaagcc cggcgtcacc gtctccatga
     1861 aatagaccag gtcgctcgcg ggcgtgccgt ggcacagcag cacgtcctcg aacagccggt
     1921 gggtggccgg cagcgccgcg agccacgcgc gttcgaccgc cccgagcgcg cgcgccgcga
     1981 acgcgtcgga cgcgcccatg cgcgcgacgt cgggcgcgag cgcctggcgc tcgtggttgc
     2041 cggcgatcgt gcgccagcgc gcgggctcgg cggcttcgcg cgccatcagc cattgcaccg
     2101 tctcccgggg ccatagcggg ccggaggcga tgtcgccgag gttgacgacg agcgccggct
     2161 gggcgcgctc gatctcggcc cagaccgcct cgagggcggg caggttgcca tggatgtcgg
     2221 aaa
//
LOCUS       DATCRO010000112        12074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_878753, whole genome shotgun sequence.
ACCESSION   DATCRO010000112 DATCRO010000000
VERSION     DATCRO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878753"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VIN75_03060"
     CDS             <1..683
                     /locus_tag="VIN75_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8973169.1"
                     /translation="RELCRTLKDAMTAEGYDVFTAASLSEGFALLTGLGARSGGAPVD
                     LVLLDLGLPDGDGEQLLAALRQKHTVPVIVISARESDGQKVRLLDAGADDYLVKPFSI
                     RELLARMRVGLRHRGTSVSPAVTRYAVGALNIDLGTREVTLAGQAVHLTPTEFNLLAR
                     LVRSAGQVVTHRQLLADVWGAEFTEHTHYLRLYMAQLRGKLEDNPADPQLLMNEPGVG
                     YRIVEPQA"
     gene            complement(732..2729)
                     /locus_tag="VIN75_03065"
     CDS             complement(732..2729)
                     /locus_tag="VIN75_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8973170.1"
                     /translation="MFSDFFHSGWTPASATLAAIGMGLAVLLLLWAIAWLTGAVRYIP
                     NDRIGVVEKLWSGSGSVKQGLLALSGEAGFQPELRRGGFHFFAPLQYRVHICPMVSVT
                     QGKIGYVFARDGVDLPAGQTLADNATITNFLDVRAFLAGGGQKGPQRKILREGTHIIN
                     PALFVVMTEETTYSMTMDATEREYYQKMRELLDQRRGFSPLVIKGNEESHGSDLLAVV
                     TVQDGPALPRDELLAPDVGDAHNSFQEPEKFLKAGGRRGRQERVLVEGTYYINRLFAT
                     VELINKTVIPVGFVGVVVSYTGRKGNDVSGVEYSHGELVESGCRGVWRDPLMPGKYAF
                     NTYAGKIELVPTTNFVLMWQTGMTGSNFDSNLREITLITKDAFEPLLPLSVVVHIDYR
                     KAPMVVQRFGNVKQLVEQTLDPMVSSYFKNVSQTRTFIELIQSRSELQEGASVDMRER
                     FQAYSLEFQEVLIGTPKPQTGDNKIENIMSQLRDRQIAREQVETFAQKQIAADKQREL
                     NESEKRAEKQAELTGSLVDISIKDNNGAALVKAAERRRQEIESLAQADRFRQENEGAG
                     RAAAIKAVGEAEASAIKAKSDALEGDGADKQLMQTVMLRLAEAFETARVPLVPQIQVG
                     GSGGEEGGNAFGTLLSMMNALKAAELSRLATPGSGGTRPAA"
     gene            3061..3612
                     /locus_tag="VIN75_03070"
     CDS             3061..3612
                     /locus_tag="VIN75_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973171.1"
                     /translation="MSPRIATATGNDVTRSPRWASVGSLGDVEATDAAHEAPAMREVR
                     KLDSLSAATKGPTARILRFVLQCVNWGAGWSEGSRIQFRDAHMIAARSWHSHAILSPR
                     IGPEIEHLARGSPAKAATESFEGCRVDVVAVAAARTIRSECRFEHVGFEQAKKVRAVV
                     RRSDARGVVGACGGRGVDDREAE"
     gene            3530..4285
                     /locus_tag="VIN75_03075"
     CDS             3530..4285
                     /locus_tag="VIN75_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973172.1"
                     /translation="MLWFAAAMQGASLAHAAGGASTTVRPSDCRPRDADSTCATAVAG
                     APAAPPPTNDPALPLYDGPLGGVPPSGYAYWGFDFIAESTGTRSPVRPDEFINRSSVP
                     VTITLSFTFPMNHPCHHDCLPGVEFEVDAGWFKVDPPYVINGNTVSVTQTFGPGRGFG
                     WVIGLWESSNPHLKVTVPQGSAATLEAVGLPSLPQLANEIPATTSQCTCSDGTTASCS
                     DGSRFSNGLLGSWTQNFQTYLRAGAFNDCQTIR"
     gene            complement(4298..5365)
                     /locus_tag="VIN75_03080"
     CDS             complement(4298..5365)
                     /locus_tag="VIN75_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8973173.1"
                     /translation="MDSLIVAGSPAGHAARRRVLATLFRAVAGAALASALHGCGGGGS
                     GAPEPPPPAPTPAPSPAPSGPIAAQMTVPTPVGYDAERLAAFNRLNEIRLSAGLGMVA
                     QSTAMDQAAQAHADWMVANDVFSHEEQTGTPGFVATDWPLRDEAFGYVPVGGGEVMSA
                     PAHGAAGVDGLINTVYHRAVMLAFEPVDVGIGWSGAAGAHVSMPLVIDMTRPGSDSVR
                     GLGQTTQPTIGGIAVWPLDHASGVPLQLGLENPNPVPSRDVLSLGTPMSVTIDRLKTI
                     STMSFVLSESGTGTVIPTRLISNQTDPNFLVPPSFVAAVPLVVLNPNTTYNATFSGIT
                     TSLEGTLTNVNRTWSFTTAAH"
     gene            5622..6872
                     /locus_tag="VIN75_03085"
     CDS             5622..6872
                     /locus_tag="VIN75_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8973174.1"
                     /translation="MRTPSWWLPEGVEPSASWLLASRMLRGFADGYVAVLLPAYLGAL
                     GFGVVQIGLLGTLTLAGSAAATIAVGALGHRFAASRLMLAAALTMVATGLGFAGLHAF
                     WPLALVAFVGTLNPSSGDVSVFLPLEHARLARAAEGSRARTALFSRYALAGGLSAAVG
                     ALASAWPAWWSAHTGGSALNAMRVMFVLYAAIGAVVALLYRGFIAQEQREHAADAASP
                     GANPDAPQPLGPSRGIVVRLAALFCIDSFAGGLLVNAMLALWLFERFGLSLSAAGQFF
                     FWSGLAMTASQLLAPVLARRIGLVNTMVFTHIPASLFLISASMASSLPVALALLLLRS
                     VLSSMDVPARNAFVMAVVTPPERAAAASFTAVPRSLASALGPTIGGGLLASGILAAPL
                     LACGTLKIVYDLLLLAMFRRHPVD"
     gene            complement(7101..8396)
                     /locus_tag="VIN75_03090"
     CDS             complement(7101..8396)
                     /locus_tag="VIN75_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8973175.1"
                     /translation="MPSDPTDSRPLQSGAAALSDLAQRERFLSALAEAVADGNAQRVL
                     LSKPVDKGDDLERVTVRPLVLRGERVLSFVFKHRTRDITKNEPVDAALATIAELLGAR
                     FAHAHLFTRDAELQLMMSRKGRWTLHRAKANTTDATRPAVAAASDDAPAPHDRQKQRY
                     VSLDAPFLVELGVTTARHELVPAMARKWKQINKFVEVMSHALESAGVGATAPLNVVDF
                     GAGKGYLTFALHDWLRRQAAGAPRMLDVVGVELRPDLVKLCNDAIGKCHLEGLRFEAG
                     DVSSFVPERLDVMIALHACDTATDHALHTGIRAGASIIMCSPCCHKQIRPQMQMPSLL
                     KPMLQHGIHLGQQAEMVTDGLRALLLEAHGYDAQVFEFVALEHTSKNKMILAVRRPGP
                     QDTPQAIRRRDDVLAQVAELKRFYGIREHCLETLLAEPA"
     gene            8518..10128
                     /locus_tag="VIN75_03095"
     CDS             8518..10128
                     /locus_tag="VIN75_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8973176.1"
                     /translation="MRLARRLLTSLSLAAGLLAALAAHAADPVINAVPRKLALAPASA
                     ASASTQGALIVGSKRFTESYILGEILRQAAAAAGPAEHRPGLGNTAVVLAALKAGAID
                     VYPEYLGTVDEEILGHRTPAPREQIDRELAQQGLGLAVPLGFSNGYALAMPADEAERL
                     HITKISDLADHPDVVMGLSHEFLGRADGWPGLAQKYGLALHPVGIDHGLSYDAMASGK
                     IAVTDIYTTDAQIAPRHLRVLADDKDYFPHYDAMLLYRLDAVTRHPAQWQALQALAGR
                     IPAATMIAMNGRAELRHETFDAIARDFLAGRIDATPTAVTGASQEGALRGRFVSRLFA
                     GDLARLLGQHLLLVAVAVGCATLLGVPLGAAAAAYPRSQGPIMATAGVLQTVPSLALL
                     AMLIPLLGRIGTAPALVALTLYALLPIARNTATGLLGVPAGLREAGTALGLTPAQRWE
                     AVDVRLALPTIMAGIGTAAVITVGTATIAAFVGAGGLGERIVTGLALNDYAMLLAGAV
                     PAAVLALAVQGVFALVERAVTPRQQAGA"
     gene            complement(10125..11519)
                     /gene="gorA"
                     /locus_tag="VIN75_03100"
     CDS             complement(10125..11519)
                     /gene="gorA"
                     /locus_tag="VIN75_03100"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY8973177.1"
                     /translation="MEMTHDLIVIGGGSGGVAGSRRAAAHGAKVAIVEADRFGGTCVI
                     RGCVPKKLMMYAAGFAHQFKDAAGFGWSDVQARFEMSRWADAKKREIDRLEKIYDDML
                     VNAGVEIVRGRGRFVSASEVDVDGRRLAAPRILVATGGAPASEAIPGLASAMTSSEVL
                     DLREVPESLLVIGGGYIAVEFASILAALGSRVTLAYRDKWPLRGFDDELRGRLALALG
                     ERGIAMACGTPLVRLERDTEGFALHRADGSVLRASAALNATGRRPSTAGLGLAEVGVQ
                     LNAKGAIVVDNESRSTAPTIWAVGDVTDRVNLTPVAIAEARAFADTEFDKDPKRVDHR
                     VVASAVFTDPPIGTVGMTEQQAALRGPVDIYAADFRPMKEAFIGGSQRAFMKLVVDGL
                     SQRVLGVQMIGPEAPEIVQSLAVAVTCGARKSDFDQTIAVHPTLAEEFVLMRTPVRRL
                     AQAVQPPAAPVAAG"
     gene            complement(11736..>12074)
                     /locus_tag="VIN75_03105"
     CDS             complement(11736..>12074)
                     /locus_tag="VIN75_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973178.1"
                     /translation="VAATELSVEVEDEDDDDEVDDVSAGIAAGAEVAGSVVVVVVVVD
                     VSLGAVLLSVLAGSVVVVVLLGAVLVSAAGVVCVVVVVVVDASLLVWATASPMPASKA
                     AAAATADNFF"
BASE COUNT         1766 a         4303 c         4139 g         1866 t
ORIGIN      
        1 accgcgagct gtgccgcacg ctcaaggacg cgatgacggc cgagggctac gacgtcttca
       61 ccgccgccag cctcagcgag ggcttcgcgc tgctcacggg gctgggcgcg cgctcgggcg
      121 gcgcgccggt cgatctcgtg ctgctcgatc tcggcctgcc cgacggcgac ggcgagcagc
      181 tgctggcggc gctgcgccag aagcacacgg tgccggtgat cgtgatctcg gcgcgcgaga
      241 gcgacggcca gaaggtgcgc ctgctcgacg ccggcgccga cgactacctc gtcaagccct
      301 tcagcatccg cgaactgctg gcgcgcatgc gcgtggggct gcgccatcgc ggcaccagcg
      361 tgtcgccggc cgtcacgcgc tacgccgtgg gcgcgctcaa catcgacctc ggcacgcgcg
      421 aggtgacact cgccggccag gccgtgcacc tcacgcccac cgagttcaac ctgctggcgc
      481 gcctcgtgcg cagcgccggc caggtcgtca cgcaccgcca gctgctggcc gacgtgtggg
      541 gcgccgagtt caccgagcac acgcactacc tgcgcctgta catggcgcag ctgcgcggca
      601 agctggagga caacccggcc gacccgcagc tgctgatgaa cgagcccggc gtgggctacc
      661 gcatcgtcga gccgcaggcc tgaagccgcg ccggcgcgcc gcgcacgcgc gcgacgctcc
      721 ggcgagggcg ctcaggcggc cggacgcgtg ccgccgctgc ccggcgtcgc gaggcgcgac
      781 agctccgcgg ccttgagggc gttcatcatc gacagcagcg tgccgaacgc gttgccgcct
      841 tcttcgccac ctgagccgcc gacctggatc tgcggcacca gcggcacgcg tgcggtctcg
      901 aaggcctcgg ccaggcgcag catcacggtc tgcatcagct gcttgtcggc gccgtcgccc
      961 tcgagcgcgt cggacttggc cttgatggcg gacgcctcgg cctcgcccac cgccttgatc
     1021 gcggcggcgc ggcccgcgcc ttcgttctcc tggcggaagc ggtcggcctg cgcgagcgac
     1081 tcgatctcct ggcggcggcg ctcggcggcc ttgaccagcg cggcgccgtt gttgtccttg
     1141 atcgagatgt ccaccagcga accggtcagc tcggcctgct tctcggcgcg cttctcgctc
     1201 tcgttgagct cacgctgctt gtcggccgcg atctgcttct gtgcaaaggt ctcgacctgc
     1261 tcgcgcgcga tctggcgatc gcgcagctgc gacatgatgt tctcgatctt gttgtcgccc
     1321 gtctgcggct tgggcgtgcc gatcagcact tcctggaact cgagcgagta ggcctggaag
     1381 cgctcgcgca tgtcgaccga cgcgccttcc tgcagctcgc tgcgcgactg gatcagttcg
     1441 atgaacgtgc gggtctgcga cacgttcttg aagtacgacg acaccatcgg gtcgagcgtc
     1501 tgctccacca gctgcttcac gttgccgaag cgctgcacca ccatcggcgc cttgcggtag
     1561 tcgatgtgca ccaccaccga cagcggcagc agcggctcga acgcgtcctt ggtgatcagc
     1621 gtgatctcgc gcaggttcga atcgaagttc gagcccgtca tgcccgtctg ccacatcagc
     1681 acgaagttgg tggtgggcac cagctcgatc ttgccggcgt aggtgttgaa cgcgtacttg
     1741 cccggcatca gcgggtcgcg ccacacgccg cggcagccgc tctccaccag ctcgccgtgc
     1801 gaatactcga cgcccgagac gtcgttgccc ttgcggcccg tgtacgacac caccacgccg
     1861 acgaagccga ccgggatcac cgtcttgttg atcagctcca ccgtcgcgaa caggcggttg
     1921 atgtagtagg tgccttcgac cagcacgcgc tcctggcggc cgcgcctgcc gccggccttg
     1981 aggaacttct cgggctcctg gaacgagttg tgcgcgtcgc cgacgtccgg cgcgagcagt
     2041 tcgtcgcgcg gcagggcggg gccgtcctgc acggtcacga cggccagcag gtccgagccg
     2101 tgcgattcct cgttgccctt gatgacgagc ggcgagaagc cgcgacgctg gtcgagcagc
     2161 tcgcgcatct tctggtagta ctcgcgctcg gtggcgtcca tcgtcatcga gtacgtcgtc
     2221 tcctcggtca tcaccacgaa cagcgccgga ttgatgatgt gcgtgccctc gcgcaggatc
     2281 ttgcgttgcg ggcccttctg gccaccgccc gcgaggaagg cgcgcacgtc caggaaattg
     2341 gtgatcgtcg cgttgtcggc cagcgtctgg cccgcgggca ggtcgacgcc gtcgcgcgcg
     2401 aacacgtagc cgatcttgcc ctgcgtcacc gacaccatcg ggcagatgtg cacgcggtac
     2461 tggagcggcg cgaagaagtg gaagccgccg cggcgcagct cgggctggaa gccggcttcg
     2521 cccgagagcg cgagcaggcc ctgcttgacc gagccgctgc cgctccacag cttctcgacg
     2581 acgccgatgc ggtcgttggg gatgtagcgc accgcgccgg tgagccaggc gatcgcccag
     2641 agcagcagca ggaccgccag tcccatgccg atcgcggcga gcgtggcgga cgcaggcgtc
     2701 cagccactgt ggaagaagtc ggagaacatg gtttccttcc tcgagtgtcg tcactcgtct
     2761 agttgtgtcc tggcgcgtgc gggccgcgat cctggcgcga tgccggtcgc tggagtcccg
     2821 ccaggcggcc ctcggacgga caggcgcacg gcgacgcgcc ggcgcgatgc gtgcggcccg
     2881 cgcccccgcg cgtcacgcgc acgcgcacga ccccggcatc gccgtcatgg cgactcgcag
     2941 gcgcacgcat gcacgcgagc acgaggccgc gcaccgaagc gttcgatgag ggaacggtga
     3001 gtgtgggatc aggcccgctc cggcgcgagg cgcaggtgtc gaaggcctgg gggtgcatgc
     3061 gtgtcgccga ggatcgccac ggcgacgggc aacgacgtca cgcgctcgcc gcgatgggcg
     3121 agcgtcgggt cgctcgggga tgtcgaggcg accgatgcgg cgcacgaggc gccggcgatg
     3181 cgagaggtac gcaagttgga ctcactgtct gcggccacca agggcccgac agcgcgcatc
     3241 ttgcgcttcg tgttgcagtg cgtcaactgg ggtgcgggct ggagcgaggg gtcgcgcatc
     3301 cagtttcgcg acgcgcacat gatcgccgcg cggagctggc attcgcacgc catcctgtcg
     3361 ccgcgaatcg ggccggaaat cgagcatttg gcgcggggca gtccagcgaa ggccgccaca
     3421 gaatcgttcg aagggtgccg cgtcgacgtc gtcgccgttg cggctgcaag aacgatcaga
     3481 agcgagtgca ggtttgaaca tgtcggcttc gagcaggcga agaaggtgcg tgctgtggtt
     3541 cgccgcagcg atgcaagggg cgtcgttggc gcatgcggcg ggcggggcgt cgacgaccgt
     3601 gaggccgagt gattgcaggc cgagggatgc ggacagtacg tgtgcgacgg cggtggcggg
     3661 ggcgccagcc gcgccaccgc cgacgaatga tccggcgctg cctctgtacg atggcccgct
     3721 gggcggcgtt ccaccgagcg gctacgcgta ttggggcttc gacttcattg ctgaatcaac
     3781 gggcacgcgt agccccgtcc gtcccgacga attcatcaat cgcagcagcg tcccggtcac
     3841 gatcacgctc agcttcacct tcccgatgaa tcatccctgc catcacgact gcctgccggg
     3901 cgtggagttc gaggtggacg caggatggtt caaggtcgat ccaccgtatg tcatcaatgg
     3961 caataccgtt tcggtcacac agacgttcgg gccagggcga ggcttcggct gggtcatcgg
     4021 cctttgggaa tcgtccaatc cacacttgaa ggtaaccgtt cctcagggtt cggccgccac
     4081 cctagaagcg gtgggtctgc cgtcactgcc tcagttggcg aatgaaatcc cagcaacgac
     4141 gagccaatgc acgtgctctg acggaacaac ggccagttgc tcagatggca gtcgcttctc
     4201 caacggacta ttgggatctt ggacgcagaa ctttcagacc taccttcgcg ccggtgcgtt
     4261 caacgactgt cagacgatcc gctaagaatg ctacgtctca atgcgccgca gtcgtaaagg
     4321 accaggttcg attcacattc gtcaaggtgc cttcgagcga cgtggtaatt cccgagaagg
     4381 tcgcgttgta ggtcgtattg ggattcagaa cgacaagcgg aacagcagcg acgaacgaag
     4441 gtgggacaag gaagttcgga tcagtctgat tggaaatcaa tcgagttgga atgacggtgc
     4501 cggtgccgct ctcgctcaga acaaagctca ttgtcgagat ggtcttcagc cgatcgatcg
     4561 tgaccgacat tggcgtcccc agtgacagta cgtcccggct tggtacagga ttcgggtttt
     4621 cgagtccgag ctgcaacggt acaccgctgg cgtgatcaag cggccacacg gcaatgccac
     4681 cgatcgtcgg ctgtgtcgtt tgtcccagtc ctcgaacgga gtctgaacct ggacgcgtca
     4741 tgtcgatgac caatggcatc gaaacatgcg cccccgcggc gcccgaccat ccaatgccca
     4801 cgtcgacagg ctcgaacgcc agcatcacgg cgcggtggta gacggtgttg atcaggccat
     4861 cgacacctgc agcgccatgc gcgggagcgg acatcacttc gccgcctccg acgggcacat
     4921 acccgaacgc ctcatcgcgt agcggccagt cggtggccac gaaccctggc gttcccgtct
     4981 gctcctcatg gctgaacaca tcattggcca ccatccagtc ggcatgcgcc tgcgccgcct
     5041 gatccatcgc ggtgctctgc gccaccatgc ccagccccgc cgacaagcgg atctcgttga
     5101 ggcggttgaa ggccgcgagg cgctcggcgt cgtagccgac gggcgtcggc accgtcatct
     5161 gcgctgcgat ggggcccgac ggagcgggag agggcgccgg cgtcggggcc ggcggcggtg
     5221 gctcgggggc gccgctgccg ccaccgccgc acccgtgcag cgccgaggcg agcgccgcgc
     5281 cggcgacggc gcgaaagagc gtcgccagca cgcgtcggcg cgcggcatgc ccggccggcg
     5341 aaccggccac gatcaacgaa tccatccgct tttctccctg aatcgacgac ttcgcatgaa
     5401 ggcgcgcggc accgtcgaac gggtccggcg cgacgacagc tcacctgaac gacgccccga
     5461 gcgacaacgc gaagccaacg caacacatgg ttccatgccc tccgcgttcc accaagccgc
     5521 cgaaccccca cttcatggga cgccgcgcgt cgtcggtgta attcggtcgg ttaatcgggc
     5581 attcggcgcg gcacgacggg cctgaccgca caatcggcca catgcgaact ccttcctggt
     5641 ggctgcccga gggcgtcgag ccgtcggcgt cgtggctgct ggccagccgc atgctgcgcg
     5701 gcttcgcgga cggctacgtg gccgtgctgc tgccggccta tcttggcgcg ctcggctttg
     5761 gcgtcgtgca gatcgggctg ctgggcaccc tgacgctggc cggctcggcg gcggcgacca
     5821 tcgcggtcgg cgcgctgggc catcgcttcg cggcctcgcg gctgatgctg gcggccgcgc
     5881 tgacgatggt ggccaccggc ctgggcttcg ccgggttgca cgcgttctgg ccgctcgcgc
     5941 tcgtggcgtt cgtcggcacc ttgaacccca gctccggcga cgtcagcgtg ttcctgccgc
     6001 tggagcacgc gcgcctggcc cgtgcggccg agggcagccg ggcgcgcacc gcgctgttct
     6061 cgcgctacgc gcttgccggc ggcctgagcg cggccgtggg cgcgctcgcc tcggcgtggc
     6121 ccgcgtggtg gagcgcgcac acgggcggtt cggcgctgaa cgcgatgcgc gtgatgttcg
     6181 tgctgtacgc cgcgatcggc gcggtcgtcg cgctgcttta ccgcggcttc atcgcgcagg
     6241 agcagcggga gcacgcggct gacgcggcca gccccggcgc caaccccgac gcgccgcaac
     6301 cgctgggccc gtcgcgcggc atcgtcgtgc gcctggccgc gttgttctgc atcgactcgt
     6361 ttgccggcgg gctgctggtc aacgcgatgc tggcgctgtg gctgttcgag cgtttcgggc
     6421 tgtcgttgtc ggccgccggc cagttcttct tctggtcggg cctggcgatg acggcctcgc
     6481 agctgctggc gccggtgctc gcgcgccgca tcgggctggt caacacgatg gtgttcacgc
     6541 acatcccggc cagcctgttc ctgatctcgg cgtcgatggc cagcagcctg ccggtcgcgc
     6601 tggcgctgct gctgcttcgc agcgtgctgt cgtcgatgga cgtgcccgcg cgcaacgcct
     6661 tcgtgatggc cgtggtgacg ccgcccgagc gcgccgccgc ggcgagcttc accgccgtgc
     6721 cgcgcagcct cgcgtcggcg ctggggccca ccatcggcgg cgggctgctc gccagcggca
     6781 tcctcgcggc gcccctgctt gcgtgcggga cgctcaagat cgtctacgac ctgctgctgc
     6841 tggcgatgtt ccggcggcac ccggtggact gagccaagcg ccagcggcgt tcacgacgcc
     6901 aacccacgcc ggggtcaggt cttgaacgtt gcggggtcag gtcttgaaag tcgcaacgga
     6961 tgccagccga ctgacgacgc cgcgccctcg cgcgtgccat gcgccgccaa gttcacccaa
     7021 ccctcatgca gaccggaaaa gtgcaggatt caagacctga ccccgcaacg ttcaagacct
     7081 gaccccacgc tgggcgagac tcaagccggc tcggccaaca acgtctccag gcaatgctcg
     7141 cgaatcccgt agaagcgctt gagctccgcg acctgcgcga gcacgtcgtc acggcgcctg
     7201 atcgcctgcg gcgtgtcctg cggcccgggg cgacgcaccg ccaggatcat cttgttcttg
     7261 ctggtgtgct ccagcgcgac gaactcgaac acctgcgcgt cgtagccatg cgcctccagc
     7321 agcagcgcgc gcaggccgtc ggtgaccatc tcggcctgct ggcccaggtg gatgccgtgc
     7381 tgcagcatcg gcttgagcag cgacggcatc tgcatctggg gccggatctg cttgtggcag
     7441 cagggcgagc acatgatgat ggacgcgccg gcgcggatgc cggtgtgcag cgcgtggtcg
     7501 gtggcggtgt cgcaggcgtg cagcgcgatc atcacgtcca ggcgctccgg cacgaagctg
     7561 ctcacgtcgc cggcctcgaa gcgcaggcct tcgagatggc acttgccgat cgcgtcgttg
     7621 cacagcttga cgaggtcggg gcgcagttcc acgccgacga catcgagcat gcgcggcgcg
     7681 cccgcagcct ggcggcgcag ccagtcgtgc agcgcgaacg tgagatagcc cttgcccgcg
     7741 ccgaagtcga cgacgttgag cggcgcggtg gcgcccacgc cggccgactc cagcgcgtgc
     7801 gacatcacct cgacgaactt gttgatctgc ttccacttgc gcgccatcgc cggcaccagc
     7861 tcgtggcgcg cggtggtgac gcccagttcg acgaggaaag gcgcgtccag gctcacgtag
     7921 cgctgcttct ggcgatcgtg cggcgcgggc gcgtcgtcgc tcgcggcggc gacggcgggg
     7981 cgcgtcgcgt cggtggtgtt tgccttggcg cgatgcaacg tccagcggcc cttgcggctc
     8041 atcatgagct gcagctcggc gtcgcgcgtg aacaggtgcg cgtgggcgaa gcgcgcgccg
     8101 agcagctccg cgatcgtcgc cagcgcggcg tcgaccggct cgttcttcgt gatgtccctg
     8161 gtgcggtgct tgaagacgaa cgacagcacg cgctcgccgc gcagcacgag cggacgcacg
     8221 gtgacgcgct cgaggtcgtc gcccttgtcc accggcttgc tcaggagcac gcgctgcgca
     8281 ttgccgtcgg cgaccgcctc ggccagtgcg gagagaaagc gttcgcgctg cgccagatcg
     8341 ctgagcgcgg cggcgccgga ctgcagggga cgggaatcgg tgggatcgga aggcaagggg
     8401 gaacgtgccg cgtcgccgcg acattcaggt aagcgaatgc tgcattttcc cgcagcacgc
     8461 gatttccggg ggcgaccgtg cgacgatagc ccccctgtcc caccgccgcg catccccatg
     8521 agactggcca ggcgcctcct cacctcgctg tcgctcgccg ccggcctgct cgccgcgctg
     8581 gccgcccacg ccgccgatcc cgtcatcaac gccgtgccgc gcaagctcgc gctcgcgccg
     8641 gcctcggcgg cgtccgcgag cacccagggc gcgctgatcg tcggctccaa gcgcttcacc
     8701 gagtcgtaca tcctcggcga gatcctgcgc caggcggccg ccgccgcggg gccggccgag
     8761 caccgtcccg gcctgggcaa caccgccgtg gtgctggccg cgctgaaggc gggcgcgatc
     8821 gacgtctacc ccgagtacct gggcacggtc gacgaggaga tcctgggcca ccgcacgccg
     8881 gcgccgcgcg agcagatcga cagggagctc gcgcagcagg gcctgggcct ggcggtgccg
     8941 ctgggcttct cgaacggcta cgcgctggcg atgcccgccg acgaggccga gcgcctgcac
     9001 atcacgaaga tcagcgacct cgccgatcac cccgacgtcg tgatgggcct gtcgcacgag
     9061 ttcctcggcc gcgccgacgg ctggccgggc ctcgcgcaga aatacgggct cgcgctgcac
     9121 ccggtcggca tcgaccacgg cctgtcgtac gacgcgatgg ccagcggcaa gatcgccgtc
     9181 accgacatct acacgaccga cgcccagatc gcgccgcgcc acctgcgcgt gctggccgac
     9241 gacaaggact acttcccgca ctacgacgcg atgctgctgt accgcctcga cgccgtcacg
     9301 cgccaccccg cgcagtggca ggcgctgcag gcgctggccg gccgcatccc ggccgcgacg
     9361 atgatcgcga tgaacggccg cgccgagctg cgccacgaga ccttcgacgc gatcgccagg
     9421 gacttcctcg ccggccgcat cgacgccacg ccgacggcgg tcaccggcgc gagccaggaa
     9481 ggcgcgctgc gcggccgctt cgtctcgcgg ctgttcgcgg gcgacctcgc gcgcctgctg
     9541 ggccagcacc tgctgctggt ggcggtggcc gtgggctgcg ccacgctgct gggcgtgccg
     9601 ctgggcgccg cggccgccgc gtatccacgc agccaagggc cgatcatggc gacggccggc
     9661 gtgctgcaga cggtgccgtc gctggcgctg ctggcgatgc tgatcccgct gctcggtcgc
     9721 atcggcacgg cgccggcgct ggtggcgctg acgctgtacg ccttgctgcc gatcgcccgc
     9781 aacaccgcca ccggcctgct gggcgtgccg gcgggcctgc gcgaggccgg caccgcgctg
     9841 ggcctgacgc cggcgcaacg ctgggaggcc gtcgacgtgc ggctggcgct gcccaccatc
     9901 atggccggca tcggcaccgc ggccgtcatc accgtgggca ccgccaccat cgccgccttc
     9961 gtcggcgccg gcggcctggg cgagcgcatc gtcacgggcc tggcactcaa cgactacgcg
    10021 atgctgctgg ccggcgccgt gcccgcggcc gtgctggcgc tggcggtgca gggcgtgttc
    10081 gcgctggtcg agcgggcggt cacgccgagg caacaggcgg gcgcctagcc cgccgccacc
    10141 ggcgccgccg gcggctgcac cgcctgcgcg agacgccgca ccggcgtgcg catcagcacg
    10201 aactcctcgg ccagcgtcgg atgcaccgcg atcgtctggt cgaagtcgct cttgcgcgcg
    10261 ccgcaggtca ccgccacggc caggctctgc acgatctcgg gtgcctcggg gccgatcatc
    10321 tgcacgccca ggacgcgttg cgacaggccg tcgaccacca gcttcatgaa cgcgcgctgg
    10381 ctgccgccga tgaaggcctc cttcatcggg cggaagtcgg ccgcgtagat gtcgaccggg
    10441 ccgcgcagcg cagcctgctg ctcggtcatg ccgaccgtgc cgatcggcgg gtcggtgaac
    10501 accgcgctgg cgacgacccg gtggtcgacg cgcttggggt ccttgtcgaa ctcggtgtcc
    10561 gcgaacgcgc gcgcctcggc gatggccacc ggcgtcaggt tgacgcgatc cgtgacatcg
    10621 cccacggccc agatcgtcgg cgccgtgctg cggctctcgt tgtcgacgac gatcgcgccc
    10681 ttggcgttca gctgcacgcc gacctcggcg agccccagtc cggcggtgct cggcctgcgg
    10741 cccgtggcgt tgagcgcggc tgacgcgcgc agcacgctgc cgtcggcgcg gtgcagtgcg
    10801 aagccctcgg tgtcgcgctc cagccgtacg agcggcgtgc cgcaggccat cgcgatgccg
    10861 cgctcgccga gcgccagcgc aagccggcca cgcagctcgt cgtcgaaccc gcgcaggggc
    10921 cacttgtccc gataggcgag cgtcacgcgg ctgcccagcg cggccaggat gctcgcgaac
    10981 tccaccgcga tgtagccgcc gccgatcacc agcagcgact cgggcacctc gcgcaggtcg
    11041 agcacctcgc tggaggtcat cgccgaagcc agaccgggga tcgcctcgga cgccggcgcg
    11101 ccgccggtgg ccacgaggat gcgcggcgcc gccagccggc ggccgtcgac gtcgacctcg
    11161 ctcgccgaga cgaagcgacc gcgtccccgc acgatctcga cgcccgcgtt gaccagcatg
    11221 tcgtcgtaga tcttctcgag ccggtcgatc tcgcgcttct tcgcgtcggc ccagcgcgac
    11281 atctcgaagc gcgcctgcac gtcgctccag ccgaagccgg cggcgtcctt gaactggtgg
    11341 gcgaagccgg ccgcgtacat catcagcttc ttgggcacgc agccgcggat cacgcaggtg
    11401 ccgccgaagc ggtcggcctc cacgatcgcg accttcgcgc cgtgcgccgc cgcgcgccgc
    11461 gacccggcca cgccacccga gccgccgccg atcacgatca ggtcgtgggt catttccatg
    11521 gactgtctcc gcgtcgaggc cgatgaacag ccgccatgct cgcagagatc gggagcccgc
    11581 gcggcgtcgc gggacaactc gacagcgccg ccgccgcgtt acgtgcgctg cgtttcctta
    11641 cagatgcggc accgcacttg ctgcaaccct ctccgccaga aggctcggcg ctgccgatcc
    11701 gcaacacatc aacctggaga gatcatgcaa agctatcaga agaagttgtc tgccgtcgcc
    11761 gccgctgccg ccttgctggc cggcatcgga ctggccgtcg cccagaccaa caacgacgcg
    11821 tcgacgacca cgacgaccac cacgcagacc acgccggccg ccgacacgag cacggcgccg
    11881 agcagcacga ccacgacgac cgacccggcc agcacgctca acagcaccgc gccgagcgac
    11941 acgtcgacca cgaccaccac caccacgacc gacccggcca cctcggcgcc cgccgcgatg
    12001 ccggccgaca cgtcgtcgac ctcgtcgtcg tcgtcctcgt cctcgacctc gacggacaac
    12061 tcggtcgcgg cgac
//
LOCUS       DATCRO010000113         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_237439, whole genome shotgun sequence.
ACCESSION   DATCRO010000113 DATCRO010000000
VERSION     DATCRO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237439"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VIN75_03110"
     CDS             <1..302
                     /locus_tag="VIN75_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973179.1"
                     /translation="KAVFPDRTALATLSMLAALAVGLGLAWALSLIYPTFSTPRQLEV
                     ATRRPVIGMISSVTTSALAAARRRDLQRVVLVMAVFFAVQGVWIVLVTKRLGHIL"
     gene            316..1212
                     /locus_tag="VIN75_03115"
     CDS             316..1212
                     /locus_tag="VIN75_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning ATPase"
                     /protein_id="HEY8973180.1"
                     /translation="MNLIEQATRRLEELRRAGIQVPTAALEPAADGASAMRVVDAPAP
                     QAPVAEPANDRSIGKTSREVTIDLDRLAALGYLTPMLSDRVLAEQIRVIKQAVLKNVE
                     DAGARGLRRNLVLVTSASAGEGKTFFSMNVAMSMAMEVDFHALLVDADVLRPSVLERY
                     GIEPGPGLLDLAARPELDLSDVLLRTNVPKLSLLPAGKQSHKSSELLSSAYIDRLLTE
                     LSERYADRLVIFDAPPILLSSGARHLASRVGQVIMVVQADQADSRTVTQAFDAVDACP
                     LVSTVLNRSSLPDPGAYGYYET"
     gene            1268..2761
                     /locus_tag="VIN75_03120"
     CDS             1268..2761
                     /locus_tag="VIN75_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HEY8973181.1"
                     /translation="MLTIAVAARAATAPPVEPTLGVSAVATDNAELASRGDKRKDVIG
                     EANAGLIVRSRGARVSLQGDVGLDFFAYARHSEPARVLPRGHLDLTSTLAEQWLYFDG
                     DLSASRTRADPLAAQSETSTSNTVSSVSLRASPYLAHDFSQTLSARLRSDTTITRNHT
                     DKAETAPDGSTYQHDVFTLVRKPTPLGVQIDGLHEDTTYQGDSASVLRTDSLQATLSA
                     APDPDVMLGVIGGREHAVFGGTTHDDTTYGGVMQWRPSPRTTFDLGAQHHYFGTGFNL
                     HFHDRLPRSAVDFSLTRAAQASPTAFGLNGPDSDPALLLGAILSSRTPDLPGGNSDQA
                     VDDLMLTRNLPGSFTQPQQVTSESAQLATRAQLNLIYSGQRNTFYASGWYQKATTLAG
                     SASVTPGLTFDSRQWGGSLGLYHRLTPRTSGAAEVEWSTIEALGTRSGDSQRQFAGTL
                     SLTQRLGPRATLSAGLRHFASHVVLNSASTTSEVRENQAFAGLRVQY"
BASE COUNT          401 a         1092 c          934 g          406 t
ORIGIN      
        1 ccaaggccgt gttcccggac cgcacggcgc tcgccacgct gtcgatgctg gccgcgctgg
       61 ccgtcggcct gggcctggcc tgggcgctga gcctgatcta tcccaccttc tcgacgccgc
      121 gccagctcga ggtcgccacc aggcggcccg tgatcggcat gatcagcagc gtcaccacgt
      181 cggccctggc ggccgctcgc cggcgcgacc tgcagcgggt cgtcctggtg atggccgtct
      241 tcttcgcggt gcagggcgtg tggatcgtcc tcgtcaccaa gcggctgggc cacatcctgt
      301 gatcgcgtgc cgaccatgaa cctcatcgaa caagccacgc gacgcctcga ggagctgcgc
      361 cgtgccggca tccaggtgcc caccgccgcc ctcgaacccg cggccgacgg ggcctcggcg
      421 atgcgggtgg tcgacgcgcc cgcgccgcaa gccccggtcg cggagcccgc caacgaccgc
      481 tccatcggca agacctcgcg cgaggtcacg atcgacctgg accgcctcgc cgccctgggc
      541 tacctcacgc cgatgctgtc ggaccgcgtg ctggccgagc agatccgcgt catcaagcag
      601 gcggtgctca agaacgtcga ggacgccggc gcgcgcggcc tgcggcgcaa cctggtgctg
      661 gtcaccagcg cctcggccgg cgagggcaag accttcttct cgatgaacgt ggcgatgagc
      721 atggcgatgg aggtcgactt ccacgcgctg ctggtggacg ccgacgtgct gcggccgtcg
      781 gtgctcgagc gctacggcat cgagccgggc ccgggcctgc tcgacctcgc ggcgcgcccc
      841 gagctggacc tgtccgacgt cctgctgcgc accaacgtgc ccaagctgtc gctgctgccc
      901 gcgggcaagc agtcgcacaa gtcgtcggag ctgttgtcca gcgcctacat cgaccgcctg
      961 ctgaccgagc tgtccgagcg ctatgccgac cgcctggtga tcttcgacgc cccgccgatc
     1021 ctgctgtcca gcggcgcccg ccacctggcc tcgcgcgtcg gccaggtgat catggtcgtc
     1081 caggccgacc aggccgactc gcgcaccgtc acgcaggcgt tcgacgccgt ggacgcgtgt
     1141 ccgctggtct cgaccgtgct gaatcgctcc tcgctgccgg atccgggcgc gtatggctac
     1201 tacgaaacct gaagcctcgc gcgcgccgcg gctcgcgcgg gcgggcgcgg gcgcgggcgg
     1261 gctggccttg ctgacgatcg ccgtcgccgc ccgggccgcg acggcgccgc cggtggaacc
     1321 gacgctgggc gtcagcgccg tggccaccga caacgccgaa ctcgcctcgc ggggcgacaa
     1381 gcgcaaggac gtgatcggcg aggccaacgc cggcctcatc gtgcgcagcc gcggcgcgcg
     1441 cgtcagcctc cagggcgacg tcggcctgga cttcttcgcc tacgcccgcc attccgagcc
     1501 tgcacgcgtg ctgccgcgcg gccacctcga cctgacctcg acgctggccg agcagtggct
     1561 gtacttcgat ggcgacctgt cggcctcgcg tacgcgcgcc gacccgctcg cggcgcagtc
     1621 cgagacgtcg acgtccaaca ccgtctcgtc ggtgagcctg cgcgccagcc cgtacctcgc
     1681 gcacgacttc tcgcagacgc tgagcgcccg cctgcgcagc gacacgacga tcacgcgcaa
     1741 ccacaccgac aaggccgaga ccgcccccga cggctcgacg taccagcacg acgtcttcac
     1801 cctggtgcgc aagcccacgc cgctgggcgt gcagatcgac ggcctgcacg aggacaccac
     1861 ctaccagggt gacagcgcct ccgtgctccg gaccgattcg ctgcaggcca cgctctcggc
     1921 ggcgcccgac cccgacgtga tgctgggcgt catcggcggc cgcgagcacg cggtcttcgg
     1981 cggcacgacg cacgacgaca ccacctatgg cggcgtgatg cagtggcggc cgtcgccgcg
     2041 caccacgttc gacctggggg cccagcacca ctacttcggc accggcttca acctgcattt
     2101 ccacgaccga ctgccccgca gcgcggtcga tttctcgctg acccgcgcgg cgcaggcctc
     2161 gcccaccgcc ttcggcctga acggtccgga cagcgatccg gcgctgctgc tcggcgccat
     2221 cctgagcagc cgcacgcccg acctgcccgg cggcaacagc gaccaggcgg tcgacgacct
     2281 gatgctcacg cgcaaccttc ccggctcgtt cacgcagccg cagcaggtca cgtcggagtc
     2341 ggcgcagctg gccacgcgcg cgcagctgaa cctgatctac agcgggcagc gcaacacgtt
     2401 ctacgcctcg ggctggtacc agaaggccac gacgctggcc ggcagtgcga gcgtcacgcc
     2461 cggcctgacc ttcgacagcc gccagtgggg cggctcgctg ggcctgtacc accggctgac
     2521 gccccggacg tcgggcgccg ccgaggtcga gtggagcacc atcgaggcgc tcgggacacg
     2581 ttcgggcgat tcgcagcgcc agttcgccgg cacgctgagc ctgacccagc gcctggggcc
     2641 gcgcgccacg ctgtcggcgg gattgcggca tttcgcctcc cacgtcgtgc tcaattcggc
     2701 gtcgaccact tcggaagtgc gcgagaacca ggccttcgcc ggcctgcggg tgcaatactg
     2761 agtccatgta cgagaagcat ttcggcctca cgggctcccc gttccagctc aatcccgacc
     2821 cggccttctt cta
//
LOCUS       DATCRO010000114         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_721238, whole genome shotgun sequence.
ACCESSION   DATCRO010000114 DATCRO010000000
VERSION     DATCRO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721238"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VIN75_03125"
     CDS             <1..1530
                     /locus_tag="VIN75_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973182.1"
                     /translation="VQIDMGRGRGPSLTTFGATLDQKIGSWSLNSKNSYTSGSVPTYG
                     LVNGGVPTTLAAYIASQVTAANNDPAVVAAAGAPATGGTASFTSSGAAITDTSMMVAQ
                     IEPWSVDKHIQSFTDETHVSGELFKGNTLTVGAYFADYSSRDLWYLGNQELVTLDARP
                     RLVDVKLDNGVLASNHGMTSSAFNFDFDGTWNGTNTAAYLADEWKLTDALRVDAGTRF
                     ENERIDGTSRNIQTGDLDGNPLTLYDNSLAYFGGGNTVYHWTGHATSWTLGANYDLSK
                     QFSTFVRLNSGAHLPMFDDVRSGLTGNGIERISQAELGFNAVTSLYTAYLSIYTNKLR
                     NSQFQQFQVGTGSSSFSATTQSTGLEFEVNLRPVRNFDVAFSGDFARGRYVDSGNLSS
                     NDVQRVPKVELRLTPSYRIPTDFGAFRLFGTWTHVGQRYSDPQNLQPLPAYDTLDLGA
                     SASLANGVDLRLAVNNATDTLGLTEGNPRVSGSGQGSGGAIMGRPLFGRTYMVSVGYS
                     F"
     gene            1548..1853
                     /locus_tag="VIN75_03130"
     CDS             1548..1853
                     /locus_tag="VIN75_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973183.1"
                     /translation="MTGVPYSPFVKSSPLVTAHFEVLVESDGRVLDVQLSRPSGLQAE
                     NVVLDGALMQALRRLHFRPADEGGRPRRGWGTIECVLRLSGPGVPPTARGPLRMVHG"
     gene            1921..2253
                     /locus_tag="VIN75_03135"
     CDS             1921..2253
                     /locus_tag="VIN75_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY8973184.1"
                     /translation="MALRVCDVAGRFFRRACARRQEGPWQISLPVGTAQFHVHAAEWT
                     PASVRFFVDDEFVGTGRQSPGYRMQLMLGLYELPERTPPGDAYPKRCSVDDVRVYRRL
                     VDDQDGAG"
     gene            2540..>3636
                     /locus_tag="VIN75_03140"
     CDS             2540..>3636
                     /locus_tag="VIN75_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8973185.1"
                     /translation="MPRLPRYWPIVAVFVAMTILQASVAAISIQLLSTVRAYVGGESL
                     YSKAQKDAQIHLLDYADTHQEVDYARFASALAYPLGDRAAREALQRPHPDIAAARQGF
                     LDGGNHPDDIDGLIFMFRWFQHVPFMSRTIATWTEGDRTIQQMRALVDRAHERIRAGD
                     TGSPELAATFRQSRELNARLTVLTSRFSAELGEASRTTQHLLLGLNTCIAALLTITGL
                     LFVRRSVRMQAASDLRMERLIHAVSEGVVTVDEQRRIVLFNEAAESMFGLSAAEALGC
                     PIDRFVAKLEIADGPESVPAGVPARPGALQALTGHRRDGRAFPIEVSLSRLQMDHGVL
                     TTMVLRDITARLLADAERRAHEALEASNKAKT"
BASE COUNT          556 a         1316 c         1233 g          531 t
ORIGIN      
        1 gtccagatcg acatgggccg cggccgcggc cccagcctga ccaccttcgg cgcgaccctg
       61 gaccagaaga tcggcagctg gagcctgaac agcaagaaca gctacacctc cggcagcgtg
      121 cccacctacg gcctggtcaa cggcggcgtg ccgaccacgc tcgcggccta catcgcgagc
      181 caggtgaccg cggccaacaa cgatccggcc gtcgtcgccg cggcgggcgc gccggccacc
      241 gggggcacgg ccagcttcac gagcagcggc gcggccatca ccgacacctc gatgatggtg
      301 gcgcagatcg agccgtggtc ggtcgacaag cacatccagt ccttcaccga cgagacgcac
      361 gtcagcggcg agctgttcaa gggcaacacg ctgaccgtcg gcgcgtactt cgccgactac
      421 tcgtcgcgcg acctctggta cctgggcaac caggaactgg tcacgctcga cgcgcgcccg
      481 cggctggtcg acgtcaagct cgacaacggg gtgctcgcgt ccaaccacgg catgacgtcc
      541 tcggccttca acttcgattt cgacggcacc tggaacggca ccaacaccgc ggcctacctc
      601 gcggacgaat ggaagctgac cgacgcgctg cgtgtggatg ccgggacgcg cttcgagaac
      661 gagcgcatcg acggcacgtc gcgcaacatc cagaccggcg acctggacgg caacccgctg
      721 accctgtacg acaacagcct ggcctatttc ggcggcggca acaccgtcta ccactggacg
      781 ggccacgcca cctcgtggac gctgggcgcc aactacgacc tcagcaagca gttcagcacc
      841 ttcgtgcgcc tgaacagcgg cgcgcacctg ccgatgttcg acgacgtgcg cagcggcctg
      901 acgggcaacg gcatcgagcg catctcgcag gccgagctgg gcttcaacgc ggtgacctcg
      961 ctgtacacgg cctacctgtc gatctacacc aacaagctca ggaactcgca gttccagcag
     1021 ttccaggtgg gcacgggcag cagcagcttc agcgcgacca cccagtcgac cggcctggag
     1081 ttcgaggtca acctgcggcc ggtgcgcaac ttcgacgtgg cgttctcggg cgacttcgca
     1141 cgcggccgct acgtcgactc gggcaacctg agcagcaacg acgtgcagcg cgtgcccaag
     1201 gtcgagctgc gcctgacgcc gtcgtaccgc atcccgaccg acttcggcgc cttccgcctg
     1261 ttcggcacct ggacgcacgt gggccagcgc tacagcgacc cacagaacct gcagccgctg
     1321 ccggcctacg acacgctgga cctcggggcc tcggccagcc tggccaacgg cgtcgacctg
     1381 cggctggccg tcaacaacgc gaccgacacg ctgggcctga ccgagggcaa cccgcgcgtc
     1441 tcgggctcgg ggcagggcag cggcggcgcc atcatgggcc ggccgttgtt cggccgcacc
     1501 tacatggtct cggtcggcta ctcgttctga cgacgaagga ggtcatcatg accggcgttc
     1561 cgtacagccc cttcgtgaag agctcgcctc tggtcacggc ccatttcgag gtgctcgtgg
     1621 agtccgatgg ccgggtgctc gacgtgcagc tgtcgcgccc gagcggcctg caggcagaga
     1681 acgtggtgct ggacggcgca ttgatgcagg cgttgcggcg cctgcatttc cggcccgccg
     1741 acgagggcgg tcgcccgcgg cggggctggg ggacgatcga gtgcgtcctg cggctgagcg
     1801 ggccgggcgt gccgcccacc gcgcgcgggc cgctgcggat ggtgcacgga tagcgggcgc
     1861 ccgtcccgct tcatccgtgg ctcgaagggc gccgggtccg tcttctcccg ggcgcatggg
     1921 ttggcgctcc gcgtctgcga cgtcgcgggg cgcttttttc gtcgcgcctg cgcgagacgc
     1981 caggaagggc cgtggcagat ttccctcccg gtcggcaccg cgcagttcca tgtccatgcc
     2041 gcggaatgga caccggcgtc ggtgcgcttc ttcgtcgacg acgagttcgt cgggaccggc
     2101 cggcaatcgc cgggctatcg gatgcagctc atgctcgggc tgtacgaact gccggagcgg
     2161 acgccgcctg gcgacgcata tccgaagcgc tgcagcgtcg acgacgtgcg cgtctaccgg
     2221 cggctggtcg acgaccagga cggggccggt tgagcttgcg gcggtggtgg cttgcacggc
     2281 agtgcgggcc cgaccgcgtt catggtcggc ccgcgcccgc cgcccgcacg tgtcgagatc
     2341 tccctacgcg ccggcaggga gggttcccct cgctggacgg gcgctcatca ggcacgatgg
     2401 ttcgcaagct cgatccgagc cgccccgctg gcggtccccc tgactccgag ccggtacgaa
     2461 aacgccccat gcgcgagctg ctgggccgtg cggcggccgg atgtgccacc ccatgaagcg
     2521 actcaacaag ctccgctcga tgccccgact gccgcgctac tggccgatcg tcgcggtgtt
     2581 cgtcgccatg acgatcctgc aggcgtccgt ggccgcgatc agcatccagc tgctgtcgac
     2641 ggtgcgcgcc tacgtgggcg gcgagagcct gtactccaag gcccagaagg acgcgcagat
     2701 ccacctgctc gactacgccg acacgcacca ggaggtcgac tacgcgcgct tcgcgtcggc
     2761 gctggcgtac cccctgggcg accgcgccgc gcgcgaggcg ctgcagcggc cccaccccga
     2821 catcgctgcc gcccgccagg gcttcctgga cggcggcaac catcccgacg acatcgacgg
     2881 gctgatcttc atgttccgct ggttccagca cgtgccgttc atgtcgcgca cgatcgcgac
     2941 gtggaccgag ggcgatcgca cgatccagca gatgcgcgcg ctggtcgatc gcgcgcacga
     3001 acgcatccgt gcgggcgaca ccggctcgcc ggagctggcg gcgacgttcc ggcagtcgcg
     3061 cgagctcaat gcgcgactga ccgtcctgac cagccgcttc tcggccgagc tgggcgaggc
     3121 ctcgcgcacg acgcagcacc tgctgctggg cctgaacacc tgcatcgccg cgctgctgac
     3181 gatcaccggg ctgctgttcg tgcgccgcag cgtgcgcatg caggccgcgt ccgacctgcg
     3241 catggagcgc ctgatccacg cggtcagcga gggggtggtc accgtcgacg agcagcgccg
     3301 catcgtgctg ttcaacgagg ccgccgagtc gatgttcggc ctgtccgccg ccgaggcgct
     3361 gggctgcccg atcgatcgtt tcgtcgcgaa gctcgagatc gccgacggcc ccgagtccgt
     3421 gccggccggc gtcccggccc ggcccggcgc gctgcaggcg ctgaccggcc accggcgcga
     3481 cggccgcgcg ttcccgatcg aggtgtcgct gtcgcgcctg cagatggacc acggcgtgct
     3541 cacgaccatg gtgctgcgcg acatcacggc gcgcctcctg gccgacgccg agcggcgcgc
     3601 ccacgaggct ctggaggcgt cgaacaaggc caagac
//
LOCUS       DATCRO010000115         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_451393, whole genome shotgun sequence.
ACCESSION   DATCRO010000115 DATCRO010000000
VERSION     DATCRO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451393"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="VIN75_03145"
     CDS             <1..1851
                     /locus_tag="VIN75_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973186.1"
                     /translation="AANLALKTSEARFRATFETAGDAILIIRHAVIIDCNRRAAEMLG
                     AGTRGGLLGQGMALFAPDKQANGQDSATLAALHLAGIRQGGMKTFEWRCQRLDGTQFD
                     AEITISLFRHNGHSLLQAVLRDVSERRRMIAALEAAKEEAERANRSKSVFLANMSHEI
                     RTPLNAVLGFTQLLMADRKLPEEAQSRLRVIHSAGNRLLGLINDVLDLAKIESGSLQV
                     LSAPFDLLQELRDLDSLYGAHARGKGLVLQSELTLAAPTVVEGDRNKVGQIVANLLGN
                     ALKFTERGHIGLHVWRDPQHAERIWFEVSDTGPGIAPGDIADLFVPFRQGAAGQEKGG
                     TGLGLALSRDMARAMGGDLTLASTPGSGTQVRFWLPLPTGRLSSAAAAVGAGAQVLDE
                     ATPCTVLVIEDDPHSRDVLVNLLRGAGCTVMQAFDGQEGLARCRAERFDIVFSDIRMP
                     HMDGVEMIGRLRADPFTRDLPVVAVSASSLEHERRFYIEAGFQDFIGKPYPFHEVYRA
                     LVDHAGVRLHPAGASGEADAGAAVPLADSLSPGLREPLRALASAASSGQLAAVARLME
                     TITPEAIGRARWQSFDEAAQAYDFQLLEERVEALLAQLDVGRTSDQPTGG"
     gene            2083..3978
                     /locus_tag="VIN75_03150"
     CDS             2083..3978
                     /locus_tag="VIN75_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973187.1"
                     /translation="MSKILIVDDTPHNIDVLSATLGEEHCELMAATSGARALELAARG
                     RPDLVLLDVMMPGMDGFEVCRRLKADAATADIPVIFVTARTDDVSQGFAVGGSDYISK
                     PINADEVRARVRYQLERRNLLEQLRELNHGLEEKVRERTAELTIANRHLRQEINERRY
                     MQDRLNYLATHDFVTRLYNRGALDAHVSELLARVQRAECDAVFLLIDIDQFRLVNETC
                     GCIAGDELLRQFAEVVSGLLDRTDFFARLGGDKFGVVIEDCGPDGGPALARRILDQLA
                     DFEFLWEERSFKMAASVAVVRIERDIVSFDQLMLVADEAAYLAKREGRGSIRVHDSSL
                     LEGDGHRESVNWALVLVDALKRNEFRAYFQRLTPLAAAADGQVHGLHIETLIRLWDPR
                     RQKLVLPSLFIAPAERYHLIGELDRWMIREVARLLGSMPDTHDRIDQVAINLSAVSIR
                     EPGLAQYVGDTLREFGVPGRLMCFEITETEAIVNLQSAGEFMQALHRLGCRFALDDFG
                     SGFASFTYLRQLPFDTIKIDGMFVRDMDTDLVHGGMVRSMTEMARLLKKPVVAEFVET
                     EAVAGLLRELGVDWAQGFHYHVPEPLTADALRASLAPVATANVVTAATAAPASGAQSA
                     TPPAALA"
BASE COUNT          600 a         1428 c         1408 g          567 t
ORIGIN      
        1 gccgccaacc tcgcgctcaa gaccagcgag gcgcgcttcc gcgccacctt cgagacggcg
       61 ggcgacgcga tcctgatcat ccgccacgcc gtcatcatcg actgcaaccg gcgcgccgcc
      121 gagatgctgg gcgccggcac gcgcggcggc ctgctgggcc agggcatggc gctgttcgcg
      181 cccgacaagc aggccaacgg ccaggactcc gccacgctcg ccgcgctgca cctggccggc
      241 atcaggcagg gcggcatgaa gacgttcgaa tggcgctgcc agcgcctgga cggtacccag
      301 ttcgacgccg agatcacgat cagcctgttc cgccacaacg gccacagcct gctgcaggcc
      361 gtgctgcgcg acgtcagcga gcgccggcgc atgatcgccg cgctggaagc cgccaaggag
      421 gaggccgagc gcgccaaccg cagcaagagc gtgttcctgg ccaacatgag ccacgagatc
      481 cgcacgccgc tgaacgccgt gctggggttc acgcagctgc tgatggccga ccgcaagctg
      541 cccgaggagg cgcagtcgcg gctgcgcgtc atccacagcg cgggcaaccg gctgctgggg
      601 ctgatcaacg acgtgctcga cctggccaag atcgagtccg gcagcctgca ggtgctcagt
      661 gcgccgttcg acctgctgca ggaactgcgc gacctcgact cgctgtacgg cgcgcatgcc
      721 cgcggcaagg gcctggtgct gcagagcgag ctgacgctgg cggcgccgac ggtggtcgag
      781 ggcgaccgca acaaggtggg gcagatcgtg gccaacctgc tcggcaacgc gctgaagttc
      841 actgagcgcg ggcacatcgg gctgcacgtg tggcgcgacc cacagcacgc cgagcgcatc
      901 tggttcgaag tcagcgacac gggcccgggc atcgcgcccg gggacatcgc cgacctgttc
      961 gtgcccttcc gccagggcgc ggccggccag gagaagggcg gcacgggcct gggcctggcg
     1021 ctgtcgcgcg acatggcccg cgcgatgggc ggcgacctca ccctcgccag cacgccgggc
     1081 agcggcacgc aggtgcgctt ctggctgccg ttgccgacgg ggcggctgtc gtcggcggcg
     1141 gccgcggtgg gcgcgggcgc gcaggtgctg gacgaggcca cgccctgcac ggtgctggtg
     1201 atcgaggacg acccgcacag ccgcgacgtg ctcgtgaacc tgctgcgcgg tgcgggctgc
     1261 accgtcatgc aggccttcga cggccaggag ggcctcgcgc gctgccgcgc cgagcgcttc
     1321 gacatcgtct tcagcgacat ccgaatgccc cacatggacg gcgtcgagat gatcgggcgg
     1381 ctgcgcgccg atccgttcac gcgcgacctg ccggtggtgg cggtgtcggc atccagcctc
     1441 gaacacgagc gccgcttcta catcgaggcg ggcttccagg acttcatcgg caagccctat
     1501 ccgttccacg aggtctaccg cgcgctggtc gatcatgccg gcgtgcggct gcatccggcc
     1561 ggcgcgtccg gcgaggccga cgcaggcgcc gcggtgccgc tggccgacag cctctcgccc
     1621 ggcctgcgcg aacccctgcg ggcgctggcg tccgcggcct ccagcggcca gctcgccgcg
     1681 gtggcgcggc tgatggagac gatcacgccc gaggcgatcg gccgggcgcg ctggcagtcg
     1741 ttcgacgagg cggcgcaggc ctacgatttc cagttgctgg aggagcgtgt cgaggcgctg
     1801 ctggcgcagc tggatgtcgg aagaacgagc gatcagccca ccggcggttg acacctcgaa
     1861 aggcaattgc gccttggcgc ggcgcgagga cacgcgtcgc cgcgcgcgct cgcgggcgct
     1921 ccgtccgata ccgcgtggcg ccggcccctc gcgggcgtgg cgcattgcac gaatctgcgt
     1981 acaaatcggg ccgaccggcc gcggaacatg tgtttacact cgggtcagac cgcacgggac
     2041 ccccggcgga tcaagcggga gtgggtggca gtgtccgcaa gagtgtcgaa aatcctgatc
     2101 gtcgacgata cgccgcacaa catcgacgtg ctcagcgcca cgctgggtga agagcactgc
     2161 gagctgatgg ccgccacgtc cggtgcccgc gcactggaac tcgcggcccg cggccggccc
     2221 gacctggtgc tgctggacgt gatgatgccg ggcatggacg gcttcgaggt ctgccgccgg
     2281 ctcaaggccg acgccgccac cgccgacatc cccgtcatct tcgtcaccgc ccgcaccgac
     2341 gacgtgagcc agggcttcgc cgtcggcggc agcgactaca tctccaagcc gatcaacgcc
     2401 gacgaggtgc gcgcccgggt gcgctaccag ctggaacggc gcaacctgct cgaacagctg
     2461 cgcgagctca atcacgggct cgaggaaaag gtgcgcgagc gcaccgccga actgacgatc
     2521 gccaaccgcc acctgcgcca ggagatcaac gagcggcgct acatgcagga ccggttgaac
     2581 tacctggcca cgcacgactt cgtcacgcgc ctgtacaacc gcggcgcgct cgacgcgcac
     2641 gtgtccgagc tgctcgcgcg cgtgcagcgc gccgagtgcg atgccgtctt cctgctgatc
     2701 gacatcgacc agttccgcct cgtcaacgag acctgcggct gcatcgcggg cgacgagctg
     2761 ctgcgccagt tcgccgaggt cgtctcgggc ctgctggatc gcaccgactt cttcgcgcgc
     2821 ctgggcggcg acaagttcgg cgtggtcatc gaggactgcg gccccgacgg cggcccggcg
     2881 ctggcccgtc gcatcctcga ccagctcgcc gacttcgagt tcctctggga ggagcgcagc
     2941 ttcaagatgg ccgcctcggt ggcggtcgtg cgcatcgagc gcgacatcgt ctcgttcgac
     3001 cagctgatgc tggtggccga cgaggccgcc tacctcgcca agcgcgaggg ccgcggctcg
     3061 atccgcgtcc acgattcgtc gctgctggaa ggcgacggcc accgcgagtc cgtcaactgg
     3121 gcgctggtgc tggtcgacgc gctcaagcgc aacgagttcc gcgcctactt ccagcgcctg
     3181 actccgctgg ccgccgccgc cgacggccag gtgcacggcc tgcacatcga gacgctgatc
     3241 cgcctgtggg atccgcgtcg ccagaagctg gtgctgccca gcctgttcat cgcgccggcc
     3301 gagcgctacc acctgatcgg cgagctcgac cgctggatga tccgcgaggt cgcgcgcctg
     3361 ctgggctcga tgcccgacac gcacgaccgc atcgaccagg tggcgatcaa cctgtcggcc
     3421 gtctcgatcc gcgagccggg cctggcgcag tacgtgggcg acacgctgcg cgagttcggc
     3481 gtgccgggcc gcctgatgtg tttcgagatc accgagaccg aggccatcgt caacctgcag
     3541 agcgccggcg agttcatgca ggcgctgcac cgcctgggct gccgcttcgc gctggacgac
     3601 ttcggctcgg gcttcgcgtc gttcacctac ctgcgccagc tgccgttcga cacgatcaag
     3661 atcgacggca tgttcgtgcg cgacatggac accgacctcg tgcacggcgg catggtgcgc
     3721 tcgatgacgg agatggcgcg cctgctcaag aagccggtgg tggccgagtt cgtcgagacc
     3781 gaggccgtcg ccggcctgct gcgcgagctg ggcgtcgatt gggcgcaggg cttccactac
     3841 cacgtgcccg agccgctgac ggccgatgcg ctgcgcgcga gcctggcgcc ggtggccacg
     3901 gcgaacgtcg tgaccgccgc gaccgcggcg cccgcctcgg gcgcgcaatc ggcgacgccg
     3961 cccgcggctc tcgcgtaggg cgggttagcc gcaggcgtaa ccc
//
LOCUS       DATCRO010000116         7187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_687788, whole genome shotgun sequence.
ACCESSION   DATCRO010000116 DATCRO010000000
VERSION     DATCRO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687788"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VIN75_03155"
     CDS             complement(<1..1454)
                     /locus_tag="VIN75_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973188.1"
                     /translation="MDQPPTFIAPAPGQQPDPHRAYQMFPVLNAVDIQRIQRFGELRT
                     FADGEFLIQAGEKSRGLFLLIRGGVNVVQRDGLGRVVPYHDYAPGEFLAEAGTLYGGN
                     SLVDARAVGDVEGFLVVPDQLRALIIAEADLGERIMRALILRRVSLIQAGASGATLIG
                     AAGSASVLRLQNFLQRNGQPHHVVTGEADPVAAQLLVQYGASRAETVAVTPDGTVLVD
                     PSETQLATALGMIDDRVCERIFDVLVVGAGPAGLSTAVYAASEGLRVAVVDCRSFGGQ
                     AGASARIENYLGFPTGISGQALAGRAFIQAQKFGAKMIIPAEVVSLDCSKAESEGVFA
                     IHLADGRRLRGRSLVCASGARYRRPALGRIEEFEGRGVWYWASGLEAKMCAGCEIVLV
                     GGGNSAGQAAVFLSAHVEKVHMMIRGPGLAASMSRYLIDRIEAAPNIQLWTNTELVEL
                     QGQLPSGLSSVKWRQGGKDGQVHERELRNVFLFVG"
     gene            1590..3878
                     /locus_tag="VIN75_03160"
     CDS             1590..3878
                     /locus_tag="VIN75_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY8973189.1"
                     /translation="MAQDEDIRIEPFTHAAGGWTSVMEVAHALREAHIPLAGTHILLK
                     QNKPDGFACVSCSWAKPADPHPAEFCEHGAKATAWELTTERCTPEFFARHTLAELESW
                     TDLELESQGRLTAPLRWDAATDKYVEVAWDEAFADIGARLKALDPKSVVFYTSGRASL
                     EASYMYALMARLYGTNNLPDSSNMCHESTSVGLPKSIGVPVGTAQLEDFKQCDCIFIM
                     GHNIGTNAPRMLHDLQECRERGVPVLVFNPLKEAGLVRFANPQKPLQLVTPADTTMNT
                     QYHQVKAGGDLAALTGLCKAIVALDDQAREAGDDAVIDHAFIEQHTHGFEAFCLHLRQ
                     VEWPDIEASSGLTRAAMESAAQVIGRSKRVLGVYGMGLTQHKSGVANVQMVANLLLLG
                     GNIGKPGAGILPVRGHSNVQGQRTVGITEKPEQVPADKLKELFDFDIPREKGLTTVDT
                     CKGLADGSVQAIVALGGNLLRAVPDGPVVERDWRRMKLSVQVATKLNRSHVVHGEVAY
                     LLPCLGRIEIDRQGGVEQAVSMEDSTGCMHGSRGMAEPASEQLLSEPAIVAGIAKATL
                     GPNKVPWDDWVADYAKVRQAIARTYPEIFHDFEQRMWQPGGFHRPLGACHREWKTPNG
                     KANFIVPPTLEEDDDMTARGPDVIRLTTLRSFDQFNTTVYGLHDRFRGIHGTRHVLMM
                     NDEDIARLGFADGQLVRATTAANDGVVREVPGLRIVRYDIPPGCAAGYFPELTPLVPL
                     WHHAVGSQVPAYKSVPIRLAPM"
     gene            4067..5758
                     /locus_tag="VIN75_03165"
     CDS             4067..5758
                     /locus_tag="VIN75_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY8973190.1"
                     /translation="MTLTTALPMTRRGALATAIAAMAWRPALALTGGDAAQAEALHVL
                     DRFAFGPAPGELERVTRMGAGAWVAEQLQPERLALPGWLADQLGALRTPGQTQRELVQ
                     GYRDAQREAREAKKAETAAPDGTKAPDPAAGERRRQVALIAYESGEERLLQALHSPRQ
                     LQEVLVDFWFNHFNVFQGKGEDRVLVESYEREAIRPHVLGRFRAMLGATAKHPAMLFY
                     LDNWLSVAPGFQPRRKAGLFGGKPAKAGGLNENYARELMELHTLGVDGGYTQQDVTEL
                     ARILTGWTMRPQQPRRRRFADATDVPAGGHGDSIFLFDPERHDNGAKTWLGRRIAPDG
                     QLEGEDALDVLAAHPSTARHIAFKLARRFVADDPPPALVDRLARTFLDRQGDLRAVMQ
                     ALADSAEFRDPAPTKFKTPYQYVLSAVRATGIVTTNVRPLMGQLAQLGQPLYGCQTPD
                     GWHDTEADWLNPNAITQRVNFATALAAGRLPLQRVDDPGAPAGNDGLKAMERQNDRAM
                     NGSQPLEGTTPPVDAATLLATLGPAITDRTRRAVESAQPDLRAALVLGSPDFMRR"
     gene            5770..7026
                     /locus_tag="VIN75_03170"
     CDS             5770..7026
                     /locus_tag="VIN75_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY8973191.1"
                     /translation="MQRRQILKSAAASLAWTAVGRTAFAATANAPLPGNRRLVVVFLR
                     GAVDGLSVVTPYTEGAYYQQRSTIALARPGQDGGLLDLDGHFGLNPNLAPLMPLWQSG
                     KLAFVHASGSPDPTRSHFDAQDYMESGTPGRKGTPDGWLNRLLGAEPAAAAPAGARPS
                     VTRGISVGANLPRIWAGPNPVANIANGARGTKPTQLDRPQVGKAFDALYAGDDAMSKA
                     YRESRQSRAEVNEAMSAAAREQMVADNGAPLPNGFPDDAGRLAQLMRRDPNVQFAFLA
                     LGGWDTHVNQGGAKGQLANRLQPLGQGLAEFAARMGPAFDDTTVLVLSEFGRTARQNG
                     TGGTDHGHGNVMWALGGNIAGGKVYGRWPGVDASALYEGRDLAVTTDFRQVLAGVCMN
                     NLRLPDDRLATVFPGFDGAALNIARA"
BASE COUNT         1111 a         2668 c         2511 g          897 t
ORIGIN      
        1 ccgacgaaca ggaacacgtt gcgcagctcg cgctcgtgca cctgcccgtc cttgccgccc
       61 tggcgccact tcacgctgga cagccccgag ggcagctggc cctgcagctc caccagctcg
      121 gtgttggtcc acagctggat gttgggcgcg gcctcgatgc gatcgatgag atagcgcgac
      181 atgctggccg ccagccccgg cccccggatc atcatgtgca ccttctcaac gtgcgccgac
      241 aggaacaccg ccgcctgccc cgccgagttg ccgccgccca ccagcacgat ctcgcagccg
      301 gcgcacatct tggcctccag cccggaggcc cagtaccaga cgccgcggcc ctcgaactcc
      361 tcgatgcggc ccagggccgg ccgccggtag cgcgcgccgc tggcgcacac caggctgcgc
      421 ccgcgcaggc ggcggccgtc ggccaggtgg atcgcgaaga cgccctcgct ctccgccttc
      481 gagcagtcga gcgacaccac ctcggccggg atgatcatct tggcgccgaa cttctgcgcc
      541 tggatgaatg cgcgccccgc cagcgcctgg cccgagatgc cggtgggaaa gcccaggtag
      601 ttctcgatgc gcgcgctggc gccggcctgg ccgccgaacg agcggcagtc gaccaccgcc
      661 acgcgcagcc cctcggacgc cgcgtacacc gccgtcgaca gccccgccgg cccggcgccc
      721 accaccagca cgtcgaagat gcgctcgcag acgcggtcgt cgatcatgcc cagcgcggtg
      781 gccagctgcg tctcgctcgg atcgaccagc accgtgccgt cgggcgtgac ggccaccgtc
      841 tcggccctgg acgcgccgta ctgcacgagc agctgcgcgg cgacggggtc ggcctcgccg
      901 gtgaccacgt ggtgcggctg gccgttgcgt tgcaggaagt tctgcaggcg cagcaccgac
      961 gcggagcccg cggcgccgat gagcgtcgca ccgctcgcgc cggcctggat cagcgagacg
     1021 cggcgcagga tcagcgcgcg catgatgcgt tcgcccaggt cggcctccgc gatgatcagc
     1081 gcgcgaagct ggtcgggcac caccaggaag ccctcgacat cgccgacggc gcgcgcgtcc
     1141 accagcgagt tgccgccgta cagcgtgccc gcctcggcca ggaactcgcc cggcgcgtag
     1201 tcgtggtacg gcacgacgcg gcccaggccg tcgcgctgca cgacgttcac gccgcccctg
     1261 atcagcagga acagcccgcg cgacttctcg ccggcctgga tcaggaactc gccgtcggcg
     1321 aacgtgcgca actcgccgaa gcgctggatg cgctggatgt cgacggcgtt gagcaccggg
     1381 aacatctggt acgcgcggtg ggggtcgggc tgctggcccg gtgcgggagc gatgaaggtc
     1441 ggcggttggt ccacgggtgc tctccggtcg cggcagggga agctcgactg tcgcacgacc
     1501 gcggccgtcg tgcatcgccc gggacatgca ccgcgccggg gcacgtgcct tgccgaccga
     1561 tcgggtcgaa cagcaacggg acgcgcgcca tggcccagga cgaagacatc cgcatcgaac
     1621 ccttcacgca cgccgccggc ggctggacgt cggtcatgga ggtggcgcac gcgctcaggg
     1681 aggcgcacat cccgctcgcg ggcacgcaca tcctgctcaa gcagaacaag cccgacggct
     1741 tcgcgtgcgt cagctgctcg tgggccaagc ccgccgaccc gcaccccgcc gagttctgcg
     1801 agcacggcgc caaggccacg gcgtgggagc tcaccaccga gcgctgcacg ccggagttct
     1861 tcgcccggca cacgctcgcc gagctcgaat cgtggaccga cctcgagctc gaatcgcagg
     1921 gccggctgac agcgccgctg cgctgggacg cggccaccga caagtacgtc gaggtcgcgt
     1981 gggacgaggc gttcgccgac atcggcgcgc ggctcaaggc gctggaccct aagtcggtcg
     2041 tgttctacac ctcgggccgc gcgtcgctgg aggcgtcgta catgtacgcg ctgatggcgc
     2101 ggctgtacgg cacgaacaac ctgcccgaca gctccaacat gtgccacgag agcacctcgg
     2161 tcggcctgcc caagagcatc ggcgtgccgg tgggcaccgc gcagctggag gacttcaagc
     2221 agtgcgactg catcttcatc atgggccaca acatcggcac gaacgcgccg cgcatgctgc
     2281 acgacctgca ggaatgccgc gagcgcggcg tgcccgtcct cgtgttcaac ccgctcaagg
     2341 aagccggcct ggtgcgcttc gccaatccgc agaagccgct gcagctggtc accccggccg
     2401 acaccacgat gaacacgcag taccaccagg tcaaggccgg cggcgacctc gcggcgctca
     2461 ccggcctgtg caaggcgatc gtcgcgctgg acgaccaggc gcgcgaggcc ggcgacgacg
     2521 cggtgatcga ccacgccttc atcgaacagc acacgcacgg cttcgaggcc ttctgcctgc
     2581 acctgcgcca ggtcgagtgg ccggacatcg aggcgtccag cgggctgacg cgcgcggcga
     2641 tggaatcggc ggcgcaggtc atcgggcgat cgaagcgcgt gctgggcgtc tacggcatgg
     2701 ggctgacgca gcacaagtcg ggcgtggcca acgtgcagat ggtggccaac ctgctgctgc
     2761 tgggcggcaa catcggcaag cccggcgcgg gcatcctgcc ggtgcgcggc cactccaacg
     2821 tgcagggcca gcgaacggtg ggcatcaccg agaagcccga gcaggtgccg gcggacaagc
     2881 tcaaggagct gttcgacttc gacatcccca gggaaaaggg cctgacgacg gtcgacacct
     2941 gcaagggcct ggccgacggc tcggtgcagg cgatcgtcgc gctcggcggc aacctgctgc
     3001 gcgcggtgcc ggacggcccg gtcgtcgaac gcgactggcg ccgcatgaag ctgagcgtgc
     3061 aggtggccac caagctcaac cgcagccacg tcgtgcacgg cgaggtggcg tacctgctgc
     3121 cctgcctggg ccgcatcgag atcgaccgcc agggcggcgt cgagcaggcc gtgtcgatgg
     3181 aggactccac cggctgcatg cacggctcgc gcggcatggc cgagccggcc agcgagcagc
     3241 tgctgtcgga gccggcgatc gtcgcgggca tcgccaaggc cacgctcggc cccaacaagg
     3301 tgccctggga cgactgggtg gccgactacg cgaaggtgcg ccaggcgatc gcgcggacct
     3361 accccgagat cttccacgac ttcgagcagc gcatgtggca gcccggcggc ttccaccggc
     3421 cgctcggcgc ctgccaccgc gaatggaaga cgcccaacgg caaggccaac ttcatcgtgc
     3481 cgccgacgct ggaggaggac gacgacatga ccgcgcgcgg cccggacgtc atccgcctga
     3541 ccacgctgcg cagcttcgac cagttcaaca ccacggtcta cggcctgcac gaccgcttcc
     3601 gcggcatcca cggcacgcgc cacgtgctga tgatgaacga cgaggacatc gcgcgcctgg
     3661 gcttcgcgga cggccagctg gtgcgcgcga ccaccgccgc caacgacggc gtcgtgcgcg
     3721 aagtgccggg gctgcgcatc gtgcgctacg acatcccgcc cgggtgcgcc gccggctact
     3781 tccccgagct gacgccgctc gtgccgctgt ggcaccacgc ggtgggcagc caggtgccgg
     3841 cgtacaagtc ggtgccgatc cggctggccc cgatgtgacg cgcgccgggt catccgatcc
     3901 cccggacgcg gggcgatttc agggtgatcc ctgagccgcg gctcagcccc ggttcaggca
     3961 aggcgccagg caacgcgact ttgccttcca gcaacgctgg gagtgtcaac atcgcctgtg
     4021 gagctgccgt ttaatgggca gcccctccac gctcgcctgc ccgccgatga ccctgacgac
     4081 cgcccttccg atgacgcgcc gcggcgcgct ggccaccgcc atcgccgcga tggcctggcg
     4141 tccggcgttg gccctcaccg gtggcgacgc ggcgcaggcc gaggcgctgc acgtgctcga
     4201 tcgcttcgcc ttcggccccg cgccgggcga gctcgagcgc gtcacgcgca tgggcgcggg
     4261 cgcgtgggtc gccgagcagc tgcagcccga gcgcctcgcg ctgccgggct ggctggccga
     4321 ccagctcggc gcgctgcgca cgcccgggca gacgcagcgc gagctggtgc agggctaccg
     4381 cgacgcgcag cgcgaggcgc gcgaggcgaa gaaggccgag acggcggcgc cggacggcac
     4441 gaaggcgccg gaccccgccg cgggcgaacg ccgccgccag gtcgcgctga tcgcctacga
     4501 atccggcgag gaacgcctgc tgcaggcgtt gcacagcccc cgccagctgc aggaggtgct
     4561 ggtcgacttc tggttcaacc acttcaacgt gttccagggc aagggcgagg atcgcgtgct
     4621 ggtggagagc tacgagcgcg aggcgatccg cccgcacgtg ctgggccgtt tccgcgcgat
     4681 gctgggtgcg accgccaagc atccggcgat gctgttctac ctggacaact ggctgtcggt
     4741 cgcgccaggc ttccagccgc ggcgcaaggc gggcctgttc ggcggcaagc cggccaaggc
     4801 cggcggcctc aacgagaact acgcgcgcga gctgatggag ctgcacacgc tcggcgtgga
     4861 cggcggctac acgcagcagg acgtcaccga gctggcgcgc atcctcaccg gctggacgat
     4921 gcggccgcag cagccgcggc gccggcgctt cgccgacgcg acggacgtgc cggccggcgg
     4981 gcacggcgac tcgatcttcc tgttcgatcc cgagcgccat gacaacggcg caaagacctg
     5041 gctgggccgc cgcatcgcgc cggacggcca gctcgaaggc gaggacgcgc tcgacgtgct
     5101 cgcggcgcat ccgtccaccg cgcggcacat cgcgttcaag ctcgcgcgac gcttcgtggc
     5161 cgacgatccg ccaccggcgc tggtcgaccg cctcgcgcgc actttcctcg acaggcaggg
     5221 cgacctgcgc gccgtgatgc aggcgctggc cgacagcgcc gagttccgcg acccggcacc
     5281 cacgaagttc aagacgccct accagtacgt gctgtcggcc gtgcgcgcga cgggcatcgt
     5341 cacgaccaac gttcgcccgc tcatgggcca gctcgcgcag ctgggccagc cgctgtacgg
     5401 ctgccagacg cccgacggct ggcacgacac cgaggccgac tggctcaacc cgaacgcgat
     5461 cacccagcgc gtgaacttcg ccaccgcgct ggccgcgggc aggctgccgc tgcagcgcgt
     5521 cgacgatccc ggcgcgcccg cgggcaacga cggcctgaag gcgatggagc gccagaacga
     5581 ccgcgcgatg aacggcagcc agccgctcga gggcacgacg ccgcccgtcg acgcggcgac
     5641 gctgctggcc acgctcggcc cggcgatcac ggacaggacg cggcgcgccg tcgaaagcgc
     5701 gcagcccgac ctgcgcgccg cgctcgttct gggcagtccc gacttcatgc ggcgttgaag
     5761 gagccctcca tgcaacgcag acaaatcctc aagtccgccg ccgccagcct cgcctggacc
     5821 gcggtgggcc gcaccgcgtt cgccgccacc gccaacgcgc ctctgcccgg caaccggcgc
     5881 ctggtggtcg tgttcctgcg cggcgcggtc gacggcctga gcgtcgtcac gccgtatacg
     5941 gagggggcgt attaccagca gcgctcgacg atcgcgctgg cgcgccccgg gcaggacggc
     6001 gggctgctcg acctcgacgg ccacttcggc ctcaacccga acctcgcgcc gctgatgccg
     6061 ctgtggcagt cgggcaagct cgcgttcgtg cacgcctcgg gctcgcccga cccgacgcgc
     6121 tcgcacttcg acgcgcagga ctacatggaa tcgggcacgc caggccgcaa gggcacgccc
     6181 gacggctggc tcaaccggct gctgggcgcc gagcccgcgg ccgccgcgcc ggcgggcgcg
     6241 cgcccgagcg tcacgcgcgg catcagcgtg ggcgcgaacc tgccgcgcat ctgggccggc
     6301 ccgaacccgg tggccaacat cgccaacggt gcgcgcggca ccaagccgac gcagctcgac
     6361 cggccgcagg tcggcaaggc gttcgatgcc ctgtacgccg gcgacgacgc gatgtccaag
     6421 gcctatcgcg aatcgcgcca gtcacgcgcc gaggtcaacg aggcgatgag cgccgccgcg
     6481 cgcgaacaga tggtggccga caacggcgcg ccgctgccca acggctttcc cgacgacgcc
     6541 ggccgcctcg cgcagctgat gcggcgcgac ccgaacgtgc agttcgcgtt cctggcgctc
     6601 ggcggctggg acacgcacgt caaccagggc ggtgcgaagg gccagctcgc caaccggctg
     6661 cagccgctgg gccagggcct ggccgagttc gcggcccgca tggggccggc gttcgacgac
     6721 accaccgtgc tggtgctgtc cgagttcggc cgcaccgcgc gccagaacgg caccggcggc
     6781 accgaccacg gccatggcaa cgtgatgtgg gccctgggcg gcaacatcgc cggcggcaag
     6841 gtctacggcc gctggcccgg cgtcgacgcg tccgcgctgt acgaaggccg cgacctcgcg
     6901 gtcaccaccg acttccgcca ggtgctcgcc ggcgtgtgca tgaacaacct gcggctgccg
     6961 gacgaccgtc tcgccacggt gttcccgggc ttcgacggcg cggcgctgaa catcgcgcgg
     7021 gcctgagggc gcgacgtcag aaccacattg ccaatacgat caacgcgccg gccacgagca
     7081 agggcgcggc cagcagcagc acctcgccgc tgcgccgcgc ggtgcgcggt cccggctggc
     7141 ggtgatacgc ccaggctccg agcgtgacag cgaccagcga acaggcg
//
LOCUS       DATCRO010000117         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_102480, whole genome shotgun sequence.
ACCESSION   DATCRO010000117 DATCRO010000000
VERSION     DATCRO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102480"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..895
                     /locus_tag="VIN75_03175"
     CDS             98..895
                     /locus_tag="VIN75_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY8973192.1"
                     /translation="MNERITQWPRQPLLNVWADDLSMDDLLARLATTGGVVFTVNPDH
                     LYHLQRNPEFLRAYRSADIITVDSHYVRLALRAQGRPVTHRLTGSDIVPAFCARQAGD
                     EHTRIFLLGARPGVAQAAREAINRKAGRELVVGAHGPSMNFVNDPAEIDGVLEMIQAS
                     GANVLLVGLGAPKQEIWISSVRHRLPQVRVMMGIGATIDYEAGAVKRAPVALRRLGLE
                     WAYRVASEPRRYLMRYVRSSKFLWWMLLDALGRYKDPFAAAGTPQVH"
     gene            928..2214
                     /locus_tag="VIN75_03180"
     CDS             928..2214
                     /locus_tag="VIN75_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973193.1"
                     /translation="MNNKPLAILSPLCLALYAAGAQAALEPFSFNVSETVKHESNVAH
                     QQNQYARADWISNSEIKAAIDEALGRGKLGVDAGANYNAYSRQDNLDSWGYHAGAQLD
                     WETIGDLSGSFGADTSRRQYIDGVTNDILPLPGQTTIQTVIEPNNQTDDHGFARIQLG
                     GPSRWQIFGGGDFNKRRFSASNFSSNDERQWSANFGTRYATSPDLVFGLVGNTTRGEF
                     PHVATFSFGNSQSTEVKRFHTRSINGTVQLKATGNSEFDASLGYTRETSDLLASRELK
                     FVNGSLNWTWTPPSHFTVKLGLKRSTDFDTSTTGLNNGVRYANSLNSPSVNNLALLDV
                     TYALTAKVNLDANATYSQRKYSDVSFNDPTTGLPTLESGTLRTSTFFFTAHWQPTRTT
                     DVSCGAGRERRSGSGSFSIFSYGDNTVRCTAAIRFD"
     gene            2217..3227
                     /locus_tag="VIN75_03185"
     CDS             2217..3227
                     /locus_tag="VIN75_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEY8973194.1"
                     /translation="MKVLVTGAAGFIGMHVVQRLAARGDRVVGIDNVNAYYDPALKEA
                     RLAELSGLPGFRFERLDIADGPALDALFAREKFDRVVHLAAQAGVRYSIENPLAYGES
                     NLAGFLNVLEACRRHPVSHLVYASSSSVYGGNAKMPFSEADSVDHPVSLYAATKKANE
                     LMAHTYSHLYGIATTGLRFFTVYGPWGRPDMAYFSFTRDILAGTPIAVFNEGRMMRDF
                     TYIDDIVDGVVAVLDKPATPDPAFDALAPHPGRSRAPYRVFNIGNQDPVQLGAFIETI
                     EKALGVQAVKVFKPMQPGDVVATYADVSALRDWTGVSPKTPLADGIGRFVDWYRGHFK
                     GR"
     gene            3365..>4352
                     /gene="rfbB"
                     /locus_tag="VIN75_03190"
     CDS             3365..>4352
                     /gene="rfbB"
                     /locus_tag="VIN75_03190"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY8973195.1"
                     /translation="MILVTGGAGFIGSNFVLDWLENHDEHVLNLDALTYAGNLANLES
                     LQGDPRHVFQHGDIGDRALVDQLLKTHRPRAIVNFAAESHVDRSILGPGAFLKTNIEG
                     TFTLLEAARAHWATLQGEDKASFRFLHVSTDEVYGSLTPDAAPFTENHPYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPLHFPEKLIPLVIVNALAGKALPVYGDG
                     LNVRDWLYVKDHCAAIRTVLAQGRVGETYNVGGLNEKTNIDIVRGICRLLDELRPDPA
                     GSRERLITFVKDRPGHDRRYAIDSSKLQRELGWSPSQTFESGIAATVDWYLAN"
BASE COUNT          747 a         1611 c         1360 g          634 t
ORIGIN      
        1 ctttcccgat gatcgattca ccgagcgccg ccatgaagac ctcttccccg ccgacctccg
       61 gccccgtggc cgtgcgcgga acgcagggag cgctttcatg aacgagcgca tcacccagtg
      121 gccgcgccag ccgctgctca acgtgtgggc cgacgacctc agcatggacg acctgctggc
      181 gcgcctggcc accaccggcg gcgtggtgtt cacggtcaac cccgaccacc tgtaccacct
      241 gcagcgcaac cccgaattcc tccgcgccta ccgctcggcc gacatcatca ccgtcgacag
      301 ccactacgtg cgtctcgcgt tgcgcgccca gggccgcccg gtcacgcacc ggctgaccgg
      361 ctcggacatc gtccccgcgt tctgcgcccg ccaggccggc gacgaacaca cgcgcatctt
      421 cctgctgggc gccaggcccg gcgtggcgca ggccgcgcgc gaagcgatca accgcaaggc
      481 cggccgcgag ctggtggtgg gcgcgcacgg gccgtcgatg aacttcgtca acgacccggc
      541 cgagatcgac ggcgtcctcg agatgatcca ggccagcggc gcgaacgtgc tgctggtggg
      601 gctcggcgcc ccgaagcagg agatctggat ctccagcgtc cgccaccggc tgccgcaggt
      661 gcgggtgatg atgggcatcg gcgcgacgat cgactacgag gcgggcgccg tcaagcgcgc
      721 gcccgtggcg ctgcgtcgcc tgggcctgga gtgggcgtac cgcgtggcct ccgagccgcg
      781 ccggtacctg atgcgctacg tacgtagctc gaagttcctg tggtggatgc tgctggatgc
      841 gctgggtcgc tacaaggacc cgttcgccgc cgccggcacg ccgcaggtgc attgatgcca
      901 cgccgtcccg gacggcagga gaagcaattg aacaacaaac ccctggcgat cctgtcgccc
      961 ctgtgcctcg ccctgtatgc cgccggcgcc caggcggcgc tcgagccgtt ctcgttcaac
     1021 gtcagcgaga ccgtcaagca cgagagcaac gtcgcgcacc agcagaacca gtacgcccgc
     1081 gccgactgga tctccaactc ggagatcaag gcggccatcg acgaggcgct ggggcgcggc
     1141 aagctgggcg tcgatgccgg cgccaactac aacgcataca gccgccagga caacctcgat
     1201 tcctggggct accacgcggg cgcgcaactg gactgggaaa ccatcggcga cctgtccggc
     1261 tcgttcggcg cggacacgag ccgccgccag tacatcgacg gcgtgacgaa cgacatcctg
     1321 ccgctgccgg gccagaccac gatccagacc gtcatcgagc ccaacaacca gaccgacgac
     1381 cacgggttcg cgcgaatcca gctgggcggc ccgtcgcgct ggcagatctt cggcggcggc
     1441 gacttcaaca agcgccgctt ctcggcgagc aatttctcgt ccaacgacga acgccagtgg
     1501 tcggccaact tcggcacgcg ctacgcgacg agccccgacc tcgtcttcgg cctggtcggc
     1561 aacacgaccc gcggcgagtt cccgcacgtg gccaccttct ccttcggcaa cagccagagc
     1621 acggaagtca agcggttcca cacgcgctcg atcaacggca cggtccagct caaggccacg
     1681 ggcaacagcg agttcgacgc cagcctgggc tacacgaggg agaccagcga cctgctggca
     1741 tcccgtgaac tcaagttcgt caatggttcg ctcaactgga cctggacgcc gccgtctcac
     1801 ttcaccgtca agctggggct caagcgcagc acggacttcg acacgagcac cacgggcctg
     1861 aacaacgggg tgcgctatgc caacagcctg aacagcccct cggtcaacaa cctggccctg
     1921 ctggacgtga cctacgcgct gacggccaag gtcaacctgg acgccaacgc gacgtattcg
     1981 cagcgcaagt actccgacgt ctcgttcaac gacccgacga cgggcctgcc gaccctggag
     2041 tccggcaccc tgcgcacgtc cacgttcttc ttcacggcgc actggcagcc gacgcgcacg
     2101 acggacgtgt cctgcggcgc cggccgcgag cggcgcagcg gtagcgggtc gttctcgatc
     2161 ttctcgtacg gcgacaatac ggtgcggtgc accgccgcga tccgattcga ctgaagatga
     2221 aagtcctggt caccggcgcg gccggattca tcggcatgca cgtcgtgcag cgcctggccg
     2281 cccgcggcga tcgcgtggtg ggcatcgaca acgtcaatgc ctactacgac ccggcgctca
     2341 aggaggcccg gctggccgag ctctccggcc tgcccgggtt ccgcttcgag cgcctggaca
     2401 tcgccgacgg ccccgcgctc gacgcgctgt tcgcgcgcga gaagttcgac cgcgtcgtgc
     2461 acctggccgc gcaggccggc gtgcgctatt ccatcgagaa cccgctggcc tacggcgagt
     2521 ccaacctcgc cggcttcctc aacgtgctgg aggcctgccg gcgccacccg gtgtcgcacc
     2581 tcgtctacgc cagcagctcc agcgtgtacg gcggcaacgc gaagatgccg ttctccgagg
     2641 ccgacagcgt cgaccacccg gtgagcctgt acgccgccac caagaaggcc aacgagctga
     2701 tggcgcacac gtacagccac ctgtacggca tcgccacgac gggcctgcgc ttcttcacgg
     2761 tctacgggcc ctggggccgg cccgacatgg cctacttctc gttcacgcgc gacatcctcg
     2821 ccggcacgcc catcgccgtg ttcaacgagg gcaggatgat gcgcgacttc acgtacatcg
     2881 acgacatcgt cgacggggtc gtcgcggtgc tggacaagcc tgccacgccc gacccggcgt
     2941 tcgacgcgct cgcgccgcac ccgggccgtt cgcgcgcgcc gtaccgcgtc ttcaacatcg
     3001 gcaaccagga tccggtgcag ctgggcgcct tcatcgagac catcgagaag gcgctgggcg
     3061 ttcaagccgt caaggtcttc aagccgatgc agcccggcga cgtggtggcc acctatgcgg
     3121 acgtgtcggc gctgcgcgac tggaccggcg tctccccgaa gacgccgctg gccgacggga
     3181 tcgggcgctt cgtcgactgg taccgcggcc atttcaaggg gcgctgaggc ccgccgcggc
     3241 gctgggcagc gctgtcgtcc cccttaatgg tgaggcggtc cgcggcaccc cttgagcgat
     3301 acttctgccc aacaacggac ggctgcgcgc cgcggtttcc ggcagggaaa accaggaaag
     3361 atccatgatc ctcgtcaccg gcggcgccgg cttcatcggc agcaatttcg ttctcgactg
     3421 gctcgagaac cacgacgagc acgtcctcaa cctcgacgcg ctgacctacg ccggcaacct
     3481 cgcgaacctc gaatcgctgc agggcgaccc gcggcacgtg ttccagcacg gcgacatcgg
     3541 cgaccgcgcg ctcgtcgacc agcttctgaa gacgcatcgc ccgcgcgcga tcgtcaactt
     3601 cgcggccgag agccatgtcg accgctcgat cctcggcccg ggcgccttcc tcaagaccaa
     3661 catcgaaggc acgttcacgc tgctcgaggc ggcgcgcgcg cactgggcca cgctccaagg
     3721 cgaggacaag gcgtcgttcc gcttcctgca cgtgagcacc gacgaggtct acggctcgct
     3781 gacgcccgac gccgcgccgt tcaccgagaa ccacccgtac gagccgaaca gcccgtactc
     3841 ggccagcaag gccgccagcg accacctcgt gcgcgcctgg caccacacgt acggcctgcc
     3901 ggtgctcacc accaactgct cgaacaacta cggcccgctg cacttccccg agaagctgat
     3961 cccgctggtg atcgtcaacg cgctcgcggg caaggcgctg ccggtctatg gcgacgggct
     4021 gaacgtgcgc gactggctgt acgtcaagga ccactgcgcc gccatccgca ccgtgctggc
     4081 ccagggccgc gtcggcgaga cgtacaacgt cggcggcctc aacgagaaga ccaacatcga
     4141 catcgtgcgc ggcatctgcc gcctgctcga cgaactgcgc cccgacccgg cgggctcgcg
     4201 cgagcgcctg atcaccttcg tcaaggatcg tcccggccac gaccgtcgct acgccatcga
     4261 ttcctccaag ctgcagcgcg agctgggctg gagtccctcg caaaccttcg agagcggcat
     4321 cgccgccacc gtcgactggt acctcgcgaa cc
//
LOCUS       DATCRO010000118         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1510, whole genome shotgun sequence.
ACCESSION   DATCRO010000118 DATCRO010000000
VERSION     DATCRO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIN75_03195"
     CDS             <1..520
                     /locus_tag="VIN75_03195"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEY8973196.1"
                     /translation="TAPTNGAAGIVPAVLHYYTRFVPQATQAGVVDFLLTAAAIGILY
                     KENASISGAEVGCQGEVGVACSMAAAGLAAVMGGTPRQVENAAEIGMEHHLGLTCDPV
                     GGLVQIPCIERNAIASVKAINAARMALRGDGTHHVSLDKVIKTMRETGADMQTKYKET
                     ARGGLAVNIVEC"
     gene            832..1227
                     /locus_tag="VIN75_03200"
     CDS             832..1227
                     /locus_tag="VIN75_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973197.1"
                     /translation="MRRHLFLAAPFLALAGCATAPPMGAALDEVRIDSRGFEAGGDAC
                     ADFALTAAQARYFLARAVVVTARQQRDDWDVLPCYVRGTARSASGVWHWEIRAGGTAT
                     LETPAGDTELRACSDCDAVLGRPAGRRRL"
     gene            complement(1250..1786)
                     /locus_tag="VIN75_03205"
     CDS             complement(1250..1786)
                     /locus_tag="VIN75_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8973198.1"
                     /translation="MKSRLILLLLAAPATAAFAQYKCTASNGAITFQQTPCPSSQTEQ
                     KLTVIPNGHPPPAAASAAASGAAAVKPANVPPPAAQPTESVDKRMLARYERQRQRDTL
                     EKAVQSAQADKDGRINARLQDIAGAKRQFGDDPANAQALRDALASIDNRYRALMELDD
                     SRIQSARKALDDWDKSSH"
     gene            complement(1931..>2408)
                     /locus_tag="VIN75_03210"
     CDS             complement(1931..>2408)
                     /locus_tag="VIN75_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973199.1"
                     /translation="GGELQAPMGRAIIGGVITSTLLTLVVVPVIYAYIEQWVGFFKQR
                     LDASSRGACVGAVLSGLVGIVLMMTGVPMAGVGAVMAAAIGLSVWACWSYATANQYHR
                     GWAAMGLLGMLGFMILAFLRPIQDGGPPEELSMAPPNTMAPDSQPDSEPEADSVMA"
BASE COUNT          321 a          895 c          870 g          322 t
ORIGIN      
        1 cacggcgccc accaacggcg ccgccggcat cgtgcccgcg gtgctgcact actacacccg
       61 cttcgtgccg caggccacgc aggccggcgt ggtcgacttc ctgctcaccg ccgcggccat
      121 cggcatcctc tacaaggaga acgcgtcgat ctccggcgcg gaggtcggct gccagggcga
      181 ggtgggcgtg gcctgctcca tggccgccgc cggcctggcc gccgtgatgg gcggcacgcc
      241 gcgccaggtc gagaacgccg ccgagatcgg catggaacac cacctgggcc tgacctgcga
      301 cccggtgggc ggcctcgtgc agatcccctg catcgagcgc aacgcgatcg catcggtcaa
      361 ggccatcaac gcggcgcgca tggcgctgcg cggcgacggc acgcaccacg tgagcctgga
      421 caaggtcatc aagacgatgc gcgagaccgg cgccgacatg cagaccaagt acaaggagac
      481 cgcgcgcggc ggcctggcgg tcaacatcgt cgagtgctga gctcgcctcg ccggaccggg
      541 gaggcaccgc cggcctcgcg catccggccc tcggcgaatc gcaggcggtt ccacgcggcc
      601 aggatggtgg cggcgagtct tgcgcggacc tcgcgctggc cgactcccgg ccccgcacat
      661 tcctcgccag ggcggcgatc gcagtcgcga cggcgccgcg cggcgggcgc gacctgcgcc
      721 cgcgccgggt acgcgcattt cgcgccgcac ccagccacgg tccgcgggcc gccccgcatc
      781 ggggtcgatg tcgatgcggg cctgcgccga aatgcgcgac actggccgac catgcgacga
      841 cacctgttcc tggccgctcc cttcctcgcc ctcgccggct gtgcgacggc gccgcccatg
      901 ggagccgcgc tcgacgaggt gcgcatcgac tcgcgcggct tcgaggccgg gggcgacgcc
      961 tgtgccgatt tcgcgctgac cgccgcccag gcgcgctact tcctcgcacg ggcggtggtg
     1021 gtcaccgcac gccagcagcg cgacgactgg gacgtcctgc cctgctacgt gcgcggcacc
     1081 gcgcgctcgg ccagcggcgt ctggcactgg gagatccgcg ccggcggcac cgccacgctg
     1141 gagacgcctg cgggcgacac cgaactgcgc gcctgcagcg actgcgacgc cgtgctgggc
     1201 cgcccggcgg gccgccgccg cctctgaggc ctgcgcggcc gtcgccggct cagtgggacg
     1261 acttgtccca gtcgtcgagc gccttgcggg ccgactggat gcggctgtcg tccagttcca
     1321 tcagcgcgcg atagcggttg tcgatggagg ccagcgcgtc gcgcagcgcc tgcgcgttgg
     1381 ccgggtcgtc gccgaactgg cgcttcgcgc cggcgatgtc ctggagccgc gcgttgattc
     1441 ggccgtcctt gtccgcctgc gcgctctgca ccgccttctc gagcgtgtcg cgctggcgct
     1501 ggcgctcgta cctggccagc atgcgcttgt cgacgctctc ggtcggctgg gccgccgggg
     1561 gcggcacgtt ggcgggcttg accgcagccg cgcccgacgc cgccgcgctc gccgcagccg
     1621 gtggcgggtg cccgttgggt atcacggtca gcttctgctc ggtctgcgag ctcggacacg
     1681 gggtctgctg gaacgtgatc gcgccgttgg acgcggtgca cttgtactgc gcgaacgccg
     1741 ccgtggccgg cgcggcgagc agcaggagga tcaggcggga cttcatgccg ccaccttagc
     1801 accgatgcgc gcggacgctg cggccgcggt cgacggatgg cgacgagggg tcgtcgaacg
     1861 gcggtggaaa tgaacaagcc gccggacgcg gacctcgcgc acggcggccg ggagcacggc
     1921 gccaggagcg tcaggccatc acggaatcgg cctcgggctc ggagtcgggc tgcgagtcgg
     1981 gcgccatggt gttgggcggc gccatcgaca gctcctcggg cgggccgccg tcctggatcg
     2041 ggcgcaggaa ggccaggatc atgaagccca gcatgcccag caggcccatg gcggcccagc
     2101 cgcggtggta ttggttggcc gtcgcgtacg accagcaggc ccacacgctc aggccgatcg
     2161 ccgcggccat caccgcgccg acgccggcca tcggcacgcc ggtcatcatc agcacgatgc
     2221 ccaccaggcc cgacagcacc gcgccgacgc aggcgcctcg cgagctcgcg tccaggcgct
     2281 gcttgaagaa gcccacccac tgctcgatgt acgcgtagat caccggcacc accaccagcg
     2341 tcagcagcgt cgaggtgatg acgccgccga tgatggcgcg gcccatcggc gcctgcagtt
     2401 cgccgccc
//
LOCUS       DATCRO010000119         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_991352, whole genome shotgun sequence.
ACCESSION   DATCRO010000119 DATCRO010000000
VERSION     DATCRO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991352"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VIN75_03215"
     CDS             <1..915
                     /locus_tag="VIN75_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY8973200.1"
                     /translation="ELKALRDEQKKLDEILGSPAALKRTVVKEIEADAKVHGDDRRTL
                     IQAEKRAVAEVKIVDEPVTVVVSLKGWVRAQKGHGHDAAGFAFKSGDGLYGTFECRTI
                     DTLLVFGSNQGGTGRVYTVAVSALPGARGDGAPITTLIDLEAGTQPLHYFAASADVTL
                     LLANTGGFGLLAKVGDMTSRQKGGKSFLSIDDADRLLPPVPLHPSHTQVACLSMSSRL
                     LVFPLDELKLQSNGGKGLTLMDVDPKDPLVSVATCANAVKVLGLGRGQKPKEEDLKGA
                     ALASHAGKRARKGKAIDAAIKGQRVVAI"
     gene            1079..1771
                     /locus_tag="VIN75_03220"
     CDS             1079..1771
                     /locus_tag="VIN75_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973201.1"
                     /translation="MRSRSLAQLAAGAAMAVAVTLPAQAEPYPAMAPLPAYLMPEADE
                     VALARSAAPAAISDAAEVRVLRQDGYATVATGTNGFVCLVERGWAGDSTFADYWNPRQ
                     RAPICFNHEAATTWLPVYLMRTKLVLAGKSRRDSLQATAAAQDAGQLPAIAAGAMCYM
                     MSRQQYLSDEDKAWHPHLMFFAPGDAAKAWGANLPGSPVIAANDPDERATIFMIEAAH
                     WSDGSPAPAAHH"
     gene            1818..2297
                     /locus_tag="VIN75_03225"
     CDS             1818..2297
                     /locus_tag="VIN75_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY8973202.1"
                     /translation="MKIRLLAACLAAFPLLASTGAHAGARQDAQVTMSPDPGERKFQQ
                     EWGYADAVVSGDLVTLSGVVAGVHPGETDLRAAYTRAFERLGGVLQRAGVSWDDVVDI
                     TSFHTDLTTQMPAIVAVKNQYVRPPFPTWTAIQVSRLIPDNGITEIKIVARKPRSDR"
     gene            2294..2686
                     /locus_tag="VIN75_03230"
     CDS             2294..2686
                     /locus_tag="VIN75_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973203.1"
                     /translation="MTRLVFYVVLIVAPLLVGFIGGAATAVIRGWGDRDVRVDVVNES
                     GQRVKSLALDVQTCGARSVLGRAALEPGDRATVRFAVCGEGGYELRAQLESGAELHGR
                     GGYVEAGYRSTERVQRSGVVSAVPALSF"
     gene            2743..3366
                     /locus_tag="VIN75_03235"
     CDS             2743..3366
                     /locus_tag="VIN75_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8973204.1"
                     /translation="MSSPRDRGRPPHPDVLTPGEWRVVEAVRHGLGNREIATRQGVSI
                     DAVKYHVANALQKLGFSSRAELRRWNGVRRDSRLHEQEIIMDPGFRLGAIGQVARTVD
                     DIAAARAWYGDVLGLPHLYSFGDLAFFDCGGVRLFLSVGDKPAAESILYFQVPDVRAA
                     HDALAARGVRFTHAPHMIHRHADGREEWMAVFDDNEGRPLAIMSTAG"
     gene            3483..4712
                     /locus_tag="VIN75_03240"
     CDS             3483..4712
                     /locus_tag="VIN75_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8973205.1"
                     /translation="MLRAMMLAAILGTMGAACPAADVALVHARVWTAPGAKPLADATV
                     LIHDGRIAAVAHRVALPKGTTVLPCGGCVVMAGFWNAHVHFIEPNWMGVATMDPATLE
                     RQLQDMLTHSGFTSVVDTGSDPRVTGELRRRIEHGDVAGPRIRSAFLPLYPEHALPYY
                     LDDLPQALRDSLPQPASPDEARESVDRNLAAGADITKLFTGSIVKPDQIQPMRTDIAT
                     AAVEESHRKGVLVFSHATNLAGVRVALDSGVDVLAHAPEVVDGIDGALMQEMVRRHVA
                     IIPTLKLFSRDDDIAGIRRLVLDAHRAGIRLVFGTDTGFLADHDVSEEFRQLSLAGLG
                     VDDILAMLTTTPAALFKVDGHAGRVARGMDGDLTVLGSDPETAGVGAFSDVRYAIRGG
                     KVLFDRARDAAPSGKHD"
     gene            4872..>5317
                     /locus_tag="VIN75_03245"
     CDS             4872..>5317
                     /locus_tag="VIN75_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973206.1"
                     /translation="MGKDFGKDVEHDEMILLFERIVGEVLKLQAKVDLLRTFGAEPEL
                     WLRNEIALNLHQVFHAHVRCETKYAGSTQRLDLHIVQQHQVYCIELKVEDRNSGSMIK
                     PLSADARKLMHFVPEAGTNKPMMRCLLAVAVSDKEGAKLASVASSD"
BASE COUNT          808 a         1912 c         1877 g          720 t
ORIGIN      
        1 gagctgaagg ccctgcgcga cgagcagaag aagctcgacg agatcctggg cagcccggcg
       61 gcgctgaagc gcacggtggt caaggagatc gaggccgacg ccaaggtcca cggcgacgac
      121 cgccgcacgc tgatccaggc cgagaagcgc gccgtcgccg aggtcaagat cgtcgacgag
      181 ccggtgacgg tggtggtgag cctcaagggc tgggtgcgcg cgcagaaggg ccacggccac
      241 gacgccgccg gcttcgcctt caagtccggc gacgggctgt acggcacgtt cgagtgccgc
      301 acgatcgaca cgctgctcgt gttcggcagc aaccagggcg gcaccgggcg cgtctacacg
      361 gtggccgtca gcgcgctgcc gggcgcgcgc ggcgacggcg cgcccatcac cacgctgatc
      421 gacctggagg ccggcacaca gccgctgcac tactttgccg catcggccga cgtcacgctg
      481 ctgctggcca acaccggcgg cttcggcctg ctggccaagg tcggcgacat gaccagccgg
      541 cagaagggcg gcaagagctt tctcagcatc gacgacgccg atcgcctgct gccgccggtg
      601 ccgctgcacc ccagccacac gcaggtcgcc tgcctgagca tgagcagccg cctgctggtg
      661 ttcccgctcg acgagctcaa gctgcagtcc aacggcggca agggcctgac gctgatggac
      721 gtcgacccca aggatccgct ggtgtcggtg gccacctgcg ccaacgccgt caaggtgctc
      781 ggcctgggcc gcggccagaa gcccaaggag gaagacctca agggcgccgc gctcgcgtcg
      841 cacgcgggca agcgcgcgcg caagggcaag gcgatcgacg cggcgatcaa ggggcagcgc
      901 gtcgtcgcga tctgaacgcg gtgcccgcct cgagaaacgc ccggcccgtc cgggcgtttt
      961 tcattgccgc gccgccgttc ggggccgcgg aaccgtcgtt cgcgccgtcc gtccttgccg
     1021 cggccgccgc gctgccgcac gatccggcca ccttcaatgt cgttcccgga ggaatgccat
     1081 gcgatccagg tccctcgcgc agctcgccgc cggcgcggcc atggccgtcg cggtcaccct
     1141 gccggcacag gccgagccct acccggcgat ggcgccgctg ccggcctacc tgatgcccga
     1201 ggccgacgag gtcgcgctcg cgcgcagcgc ggcgcccgca gccatctcgg acgcagccga
     1261 ggtgagggtg ctgcgccagg acggctacgc caccgtggcg acgggcacca acggcttcgt
     1321 gtgcctggtg gagcgcggat gggccggcga ctcgaccttc gcggactact ggaacccgcg
     1381 ccagcgcgcg ccgatctgct tcaaccacga ggccgcgacg acctggctgc cggtctacct
     1441 gatgcgcacg aagctcgtgc tggcgggcaa gtccaggcgt gacagcctgc aggccaccgc
     1501 ggcggcgcag gacgccggac agttgcccgc catcgccgcg ggcgccatgt gctacatgat
     1561 gtccaggcag cagtacctca gcgacgagga caaggcctgg cacccgcacc tgatgttctt
     1621 cgcgccgggc gatgccgcca aggcctgggg cgccaacctg ccgggctcgc cggtgatcgc
     1681 cgccaacgac cccgacgaac gcgccacgat cttcatgatc gaggcggcgc attggtccga
     1741 cggcagcccg gcgcccgccg cgcaccactg acaacccggc gatcgccccc tcgatcccac
     1801 ccgacctgga ggactccgtg aagatccgac tgctcgccgc ctgcctggcc gccttcccgt
     1861 tgctggcctc gaccggcgcc cacgccggcg cgcgccagga cgcgcaggtc acgatgtcgc
     1921 ccgatcccgg cgaacgcaag ttccagcagg agtggggcta tgccgacgcg gtggtcagcg
     1981 gcgacctggt cacgttgtcc ggcgtggtcg ccggcgtgca ccccggcgag accgacctgc
     2041 gcgccgccta cacgcgcgcc ttcgagcggc tgggcggcgt gctgcagcgc gccggcgtct
     2101 cgtgggacga cgtcgtcgac atcaccagct tccacaccga cctcaccacg cagatgccgg
     2161 cgatcgtcgc ggtcaagaac cagtacgtga ggccgccgtt cccgacgtgg acggcgatcc
     2221 aggtgtcgcg gctcatcccg gacaacggca tcaccgagat caagatcgtg gcccgcaagc
     2281 cgcgcagcga ccgatgacgc ggctcgtgtt ctacgtggtg ctcatcgtgg cgccgctgct
     2341 cgtgggcttc atcggcggtg cggccaccgc ggtcattcgc ggctggggcg accgggacgt
     2401 gcgagtcgac gtcgtcaacg aatccgggca gcgcgtgaaa tcgctcgcgc tggacgtcca
     2461 gacctgcggc gcccgcagcg tgctgggccg cgcggcgctg gagccgggcg accgcgcgac
     2521 ggtgcgcttc gcggtgtgcg gcgagggcgg ctacgaactg cgcgcgcagc tcgaaagcgg
     2581 cgccgagctg catgggcgcg gcggctacgt cgaagccggc taccgctcga ccgagcgcgt
     2641 gcagcgatcg ggcgtcgtgt ccgccgtccc ggcgctgtcg ttctgagcgg ccatgcggcg
     2701 ccgggtaggg aaattcgacg gcggcggcgc taagctgcgg ccatgtccag cccccgcgac
     2761 cgcggacgcc cgccgcaccc cgacgtcctg acgccgggcg agtggcgcgt cgtcgaggcc
     2821 gtgcggcacg gactgggcaa ccgcgagatc gccacgcgcc agggcgtgag catcgatgcg
     2881 gtgaagtacc acgtggccaa cgcattgcag aagctcgggt tctccagccg cgccgaactg
     2941 cggcgctgga acggcgtgcg gcgcgacagc cggcttcacg aacaggagat catcatggac
     3001 cccggattca ggctcggcgc gatcggccag gtcgcgcgaa cggtggacga catcgcggcg
     3061 gcgcgcgcct ggtacggcga cgtgctgggc ctgccgcacc tgtattcgtt cggcgacctc
     3121 gcgttcttcg attgcggcgg cgtgcggctg ttcctgtcgg tgggcgacaa gcccgcggcc
     3181 gagtccatcc tgtacttcca ggtgcccgac gtccgcgccg cgcacgacgc gctggcggcg
     3241 cgcggcgtgc gcttcacgca cgcgccgcac atgatccatc gccatgcgga cggccgcgag
     3301 gagtggatgg ccgtcttcga cgacaacgag ggccggccgc tcgcgatcat gtccacggcc
     3361 ggatgacgcc gcgccgcgcc cgcatgggca gggcgcgcga taccatgtgc cgtacctggg
     3421 cgacgccgat cgcgcgcccg tcgaacgacc ctgcgccgag gcgcgaaagg aagaacgtga
     3481 cgatgctgcg cgcgatgatg ctggccgcga tcctgggcac gatgggcgcg gcgtgcccgg
     3541 ccgccgacgt cgcgctcgtc cacgccaggg tctggacggc gcccggtgcg aagccgctcg
     3601 ccgatgccac ggtgctgatc cacgacggcc gcatcgccgc cgtggcgcac cgcgtcgcac
     3661 tgcccaaggg aacgacggtg ctgccctgcg gcggctgcgt cgtgatggcg ggcttctgga
     3721 acgcgcacgt gcacttcatc gagccgaact ggatgggcgt ggcgacgatg gatcccgcca
     3781 cgctcgagcg ccaactgcag gacatgctga cgcattccgg cttcacatcc gtggtcgaca
     3841 cgggatcgga tccgcgcgtc acgggcgagc tgcggcgccg catcgagcac ggcgacgtgg
     3901 cgggcccgcg catccgctcg gccttcctgc cgctgtatcc cgagcacgcc ttgccgtact
     3961 acctggacga cctgccgcag gcgctgcgcg acagcctgcc gcagcccgcg tcgcccgacg
     4021 aggcgcgcga gtcggtcgac cggaacctgg ccgccggcgc ggacatcacc aagctgttca
     4081 cgggctccat cgtcaagccc gaccagatcc agccgatgcg caccgacatc gcgacggccg
     4141 cggtcgagga gtcgcaccgc aagggcgtgc tcgtgttctc gcacgcgacg aacctggcgg
     4201 gcgtgcgggt ggcgctcgac agcggcgtcg acgtgctggc gcacgcgccg gaggtcgtgg
     4261 acggcatcga cggcgcgctc atgcaggaga tggtgcgcag gcatgtcgcc atcatcccga
     4321 cgctcaagct gttctcgcgc gacgacgaca tcgccggcat ccgcaggctc gtgctcgacg
     4381 cccaccgggc cggcatccgc ctggtcttcg gcaccgacac cggcttcctc gccgaccacg
     4441 acgtctcgga ggaattccgc cagctgtcgc tcgccggcct gggcgtcgac gacatcctgg
     4501 ccatgctcac cacgacgccc gcggccttgt tcaaggtcga cgggcacgcc gggcgcgtgg
     4561 cgcgcggcat ggacggcgac ctcaccgtgc tcggctccga tccggagact gcgggcgtgg
     4621 gcgcgttctc cgacgtccgc tatgcgatcc gtggcggcaa ggtgctgttc gatcgcgcgc
     4681 gcgacgcggc gccctccggc aagcacgact gatcgccgcg gccgctccct tcccgggacg
     4741 gcggcgcgcc ccccaaggca aggcatggtg ccgccagcca ggctcgggca tcatcccggg
     4801 atagggccgg cggtattccg cggccgaccg gccggcgcac gtccgagccg actcgacagg
     4861 gagatggaca gatgggcaag gatttcggca aggacgtcga acacgatgaa atgatcctgc
     4921 tgttcgagcg gatcgtcggc gaggtcctca agctgcaggc caaggtcgac ctgctgcgca
     4981 ccttcggcgc ggagcccgag ctgtggctgc gcaacgagat cgcgctgaac ctgcaccagg
     5041 tgttccacgc gcatgtccgg tgcgagacga agtacgccgg ctcgacgcag cggctggacc
     5101 tgcacatcgt gcagcagcat caggtgtact gcatcgagct caaggtcgag gatcgcaact
     5161 ccggcagcat gatcaagccg ctgagcgcgg acgccaggaa gctgatgcac ttcgtgcccg
     5221 aggcggggac gaacaagccc atgatgcgct gcctgctcgc cgtcgcggtg tcggacaagg
     5281 agggcgccaa gctcgcctcg gtcgccagct ccgacat
//
LOCUS       DATCRO010000120         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_496458, whole genome shotgun sequence.
ACCESSION   DATCRO010000120 DATCRO010000000
VERSION     DATCRO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496458"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIN75_03250"
     CDS             complement(<1..203)
                     /locus_tag="VIN75_03250"
                     /inference="COORDINATES: protein motif:HMM:NF017059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEY8973207.1"
                     /translation="MTPFAFELKDTSLPLVTVVLHQADVAVLAEELQLKLGETPNFFD
                     DDPVVIDLAPLQESSEPLAFADIA"
     gene            317..694
                     /locus_tag="VIN75_03255"
     CDS             317..694
                     /locus_tag="VIN75_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973208.1"
                     /translation="MKKTSPAPPAPPVAARPERWRIDAGDADVATLVIPADSLRTRRF
                     EIDCRLVVMRLADGATHGMRVDVDGDLEWTRKAPTENPGHTDSMDYHFRRELPVGRPL
                     RIVVKTQARQARRIALTIEADES"
     gene            908..1525
                     /locus_tag="VIN75_03260"
     CDS             908..1525
                     /locus_tag="VIN75_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973209.1"
                     /translation="MKIRFVAAALVAALAPVLSHAQDAAPASPVSVHPVLGFMGTFGG
                     DKLVDVLYTDGTTSAVTAGGTVYLYGGAELRTTDLPLPLSVKATVGYHVATAGGSNGD
                     YRFERIPFELLGEVDVIPNRLRLGGGLRYDTGVNLSSHGVVAAPATRFKDAAGGVFQA
                     EWMLSNNFSIVGRYVAIRYRFDDSSTGAQYHANGNQGGIGINWYL"
     gene            1655..2284
                     /gene="pssA"
                     /locus_tag="VIN75_03265"
     CDS             1655..2284
                     /gene="pssA"
                     /locus_tag="VIN75_03265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY8973210.1"
                     /translation="MDEHNAKPKPFSMIREFHLADWFTLANAFCGVGALFAVMTFLQT
                     NDVRHIYFACGLIPAALVFDVLDGRIARWRQKSSELGRELDSLADVISFGVAPAAIAY
                     GCGMQGLWDRVVLVFFVVCGVSRLARYNITAVKLSDGGDKVKYFEGTPIPTSLLLVIV
                     LWIAAVQGGVGDALWGGVLHVGGFRLHPLVLMFALSGSLMISRIRIPKL"
     gene            2486..2971
                     /locus_tag="VIN75_03270"
     CDS             2486..2971
                     /locus_tag="VIN75_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8973211.1"
                     /translation="MPRATPSSNAADVRADAWLALDKQLCFALYSASLAMTKVYKPLL
                     APLGLTYPQYLVMLALWEHDGQSVSALGDRLALDSGTLTPLLKRLETQGRISRERDAE
                     DERRVLVRLTAAGRALKVEARRIPREIGRASGCELTDLTQRLQQLRRQLLDDAPDAAH
                     A"
BASE COUNT          462 a         1116 c         1002 g          466 t
ORIGIN      
        1 gcgatgtccg cgaacgcgag cggctcgctc gactcctgca gcggcgcgag gtcgatgacg
       61 acggggtcgt cgtcgaagaa attgggggtc tcgcccagct tgagctgcag ttcctccgcg
      121 aggacggcca cgtcggcctg gtgcagcacc acggtgacca gcggcaggct ggtgtccttc
      181 agttcgaacg caaacggagt cactgtcgag tcggcgggga gggacgccgg aacgcgcaaa
      241 gcgccggagt ttaccgggtc ggcatctcgc gcctgtaacc gccggggtcg tgccgcttgc
      301 gccatgatgc gggcccatga agaaaacgtc tcccgccccg cccgctccgc cggtcgccgc
      361 caggcccgag cgctggcgca tcgacgccgg cgacgccgat gtcgccacgc tcgtgatccc
      421 ggccgactcg ctgcgcaccc gccgcttcga gatcgactgc cgtctcgtcg tcatgcggct
      481 ggccgatggc gccacgcacg gcatgcgcgt cgacgtcgac ggcgacctcg aatggacgcg
      541 caaggcgccc accgagaacc ccggccacac cgattcgatg gactaccatt tccgccgcga
      601 gctgcccgtg ggccggccgc tgcgcatcgt cgtgaagacg caggcccggc aggcacgccg
      661 catcgcgctg accatcgagg ccgacgagag ctgacgccgc caggcgctgt cgcgggcgcg
      721 cgtcgaccgc gcgagaacaa gcgcacgctt tcggtgcgga cagcttgacg caagccttgg
      781 ggtgcggggc ccccccgaga attcgcccca catcgcgcag ggaggacttg cggcggtgag
      841 gccggcaccc cgccggccgg agacgccacg cggcgctcca cacacccatc aagacagcac
      901 acatcacatg aagattcgct tcgtcgcggc cgccctcgtg gccgccctcg cccctgttct
      961 ctcgcacgcg caggatgccg cgccggcctc gcccgtgtcc gtgcacccgg tgctgggctt
     1021 catgggcacc ttcggcggcg acaagctcgt cgacgtgctc tacacggacg gcacgacctc
     1081 ggcggtcacc gccggcggca ccgtctacct gtacggcggc gccgagctgc gcaccaccga
     1141 cctgcccctg ccgctgtccg tcaaggccac ggtcggctac cacgtcgcca ccgccggcgg
     1201 cagcaacggc gactaccgct tcgagcgcat cccgttcgag ctgctgggcg aagtcgacgt
     1261 gatcccgaac cgcctgcgcc tgggcggcgg cctgcgctac gacaccggcg tcaacctgag
     1321 cagccacggc gtcgtcgccg cgccggccac caggttcaag gacgccgcgg gcggcgtgtt
     1381 ccaggccgaa tggatgctga gcaacaactt cagcatcgtc ggccgctacg tcgccatccg
     1441 ctatcgcttc gacgacagca gcaccggcgc gcaataccac gccaacggca accagggcgg
     1501 catcggcatc aactggtacc tgtaagaccg ggtcgatccg accctcgagg gcctgcgcga
     1561 tgcaggccct tttcattggg cccccggccg gggtgccgcg gcgtcttcga gccgttgcgc
     1621 ggcccgggcc cgccgagtgc cgtatcctgc ggccatggac gaacacaacg ccaagcccaa
     1681 gccgttctcg atgatccgcg agttccacct cgcggactgg ttcacgctcg ccaacgcctt
     1741 ctgcggcgtc ggcgcgctgt ttgcggtgat gacgttcctg cagaccaacg acgtccggca
     1801 catctatttc gcatgcgggc tcatcccggc cgcgctcgta ttcgacgtgc tcgatgggcg
     1861 catcgcgcgc tggcgccaga agagttccga gctcggccgc gagctcgact cgctcgccga
     1921 cgtcatctcg ttcggcgtcg cccccgcggc gatcgcgtac ggctgcggca tgcaggggct
     1981 gtgggatcgc gtcgtgctcg tgttcttcgt cgtgtgcggc gtctcgcgcc tggcgcgcta
     2041 caacatcacc gcggtcaagc tgtccgacgg cggcgacaag gtcaagtatt tcgagggcac
     2101 gcccattccc acctcgctgc tgctggtgat cgtgctgtgg atcgccgcgg tgcagggcgg
     2161 cgtcggcgat gcgctgtggg ggggcgtgct gcacgtgggc gggttccgcc tgcatccgct
     2221 ggtgctgatg ttcgcgctgt ccgggtcgtt gatgatctcg cgcatccgca tcccaaaact
     2281 gtgagcccca cggtcgctgt cccccggagg gggaggcttc gcgcgcgctc cctgccccca
     2341 aggggccggc cgcgaacggc ccggcaaagc cggttccgtc gctccctcga atctcgcttg
     2401 aggcaccccg agcggcagcg ccgccaagaa tgacgaaaga ccatttaagt tgtacgcaat
     2461 tcaattgcac gctacagttc actccatgcc tcgcgccacc ccctcctcca acgccgccga
     2521 tgtccgggcc gacgcctggc tcgcgctcga caagcagctg tgcttcgcgc tgtactcggc
     2581 gtcgctggcg atgaccaagg tctacaagcc gctgctcgcg ccgctggggc tgacgtaccc
     2641 gcagtacctg gtgatgctgg cgctgtggga gcacgacggc cagtccgtca gcgcgctcgg
     2701 cgaccggctc gcgctcgatt cgggcacgct cacgccgctg ctcaagcgcc tggagacgca
     2761 gggccgcatc agccgcgagc gcgacgccga ggacgagcgc cgcgtgctcg tgcgcctgac
     2821 tgccgccggc cgcgcgctga aggtcgaggc ccgccgcatc ccgcgcgaga tcggccgcgc
     2881 ctcgggctgc gagctgaccg acctgaccca acgcctgcaa cagctgcgcc ggcagctgct
     2941 cgatgacgcg ccggatgcgg cgcatgcgtg atcgccgacg gcgatcgatc gttcaaccac
     3001 caccaccacg aaggaagaca ccatgaagct cgagaaagtc ctgtac
//
LOCUS       DATCRO010000121        12144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_170034, whole genome shotgun sequence.
ACCESSION   DATCRO010000121 DATCRO010000000
VERSION     DATCRO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170034"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VIN75_03275"
     CDS             <1..515
                     /locus_tag="VIN75_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY8973212.1"
                     /translation="LFPVAYTPGKAPAAAPGAPTPPDGLVAQPVTFDGHVEAPRTEWF
                     IAGTETARVAMASAAGSAVRLIASPDDKSVIALDPDMPPAVQRLRFEAVAPVPAGAWW
                     RLDGKPLGAARPLPWAPWPGHHELALVDATGKALDTVTFDVRGAQAKPPGAPTRKPAR
                     SLGAAARPGA"
     gene            741..2834
                     /locus_tag="VIN75_03280"
     CDS             741..2834
                     /locus_tag="VIN75_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8973213.1"
                     /translation="MSASYEVRGAVAVITLNNPPVNGLGHETRKAVAEGIEKAEDDAA
                     VKAIVITGAGKAFSGGADIREFGSPKALAEPNLLSLIARVEGASKPVVAAVHTVCMGG
                     GLELALGCHYRVASSGASIALPEVKLGILPGAGGTQRLPRAIGVEPALNMIVSGEPVK
                     SEHLALIPGQKLFDRVYERDFMDSAIAFASEIAAVRPLPMIRDIKCKHPEGDAYFGFA
                     RNTAKAMAKNFPAPARCVDCVEQATKAKFEDGLKYEREAFAALMLTPESKALRHAFFA
                     ERAASKVPDIPEDTPVRPVAKVAIIGAGTMGGGIAMNFLNAGIPVVMLEMKQEALDKG
                     LAIIRKNYEAQVKKGKLKEDKYAERMALLSTTLSYDDLRDADLVIEAVFEEIGVKQKV
                     FEQLDAVMKPGAILASNTSTLDLDKIASFTKRPQDVIGMHFFSPANVMKLLEVVRGAR
                     TAPDVLMTVMKLGKVIRKTCVVSGVCDGFIGNRMIEQYSRQAGFLLEEGCTPQQVDKA
                     IEKFGFAMGPFRMGDLAGNDIGWAIRKRRYVEKPGQRYSKTADLLCELGRFGQKTGAG
                     WYDYVPGKRDAIPSKLVEDMLVKHREELGIAPRKISDDEIVHRLVYALVNEGAHIVEE
                     GIALRASDIDMVYLTGYGFPLWRGGPMNYADTVGLWNVAQAMKKFAKNPADDQAFWQP
                     APLLARLAAEGKSFS"
     gene            2846..3007
                     /locus_tag="VIN75_03285"
     CDS             2846..3007
                     /locus_tag="VIN75_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973214.1"
                     /translation="MPGSVKRAAAVVAGVVLGMAIAAGASPWRELFKPSEPVQPAPIQ
                     RERAPDTRV"
     gene            3051..4226
                     /locus_tag="VIN75_03290"
     CDS             3051..4226
                     /locus_tag="VIN75_03290"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY8973215.1"
                     /translation="MTEAVIVSTARTPLCKSWKGAFNMTHGAELGGHAVKAALERAGI
                     DGAEVEDVLMGCANPEGATGANIARQVALRAGLPVSVAGVTVNRFCSSGLQTIAMAAQ
                     RIIANEGDVYVAGGVESISCVQQEMNHHMLRDMWLARNKPAVYMSMLETAETVAKRYK
                     ISRERMDQYGAASQQKATAALEKGLFKDEIAPITVTAGVADPKNGIRTREVTVENDEG
                     IRPGTTYDGIKDIRPAVPGGVISAGNASQFSDGGGACVVVSSHYAERKGLKPLGRFLG
                     FAVAGCEPDEMGIGPVYAVPKVLKRLGLTVADIDLWELNEAFAVQVLYCADTLGIPMD
                     RLNVNGGAIAVGHPYGVSGQRLTGHALIEGKRRGAKKVCVTMCIGGGMGAAGVFEVL"
     gene            4256..4672
                     /locus_tag="VIN75_03295"
     CDS             4256..4672
                     /locus_tag="VIN75_03295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8973216.1"
                     /translation="MIDLDSVRSFFARAPFMVDLGVVPTAVSEGRCETELVLQQRHLQ
                     HTGQAHAGVVTAMADHTAGAAAQSVIKDGGVAITAELKTSLLRPAVGSRLVCVGNVIK
                     AGRTLIFAESDVFAILGEQRTLVAKLSATLAVVQPT"
     gene            4669..6480
                     /locus_tag="VIN75_03300"
     CDS             4669..6480
                     /locus_tag="VIN75_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8973217.1"
                     /translation="MSTRQSLDFLLYDWLKIDDLTTRERFADHSRETFDAVLDTVERL
                     AREKFAPFNRLVDTEEPRVDGERVRLPQATHDALRAYADTGMIAASQDAGIGGMQLPC
                     TVEKAANAFLSQACIGMGGYVMLTHGNAALLMAHGTPRQRDVFAAREFSGEWFGTMCL
                     SEPQAGSSLSDIATRAVPDGADFETDPLGPRYKLVGNKMWISAGEHEITPNIVHLVLA
                     KIPGPDGKLPAGVKGISLFIVPKHRVDAEGRLTGQRNDVSLAGLNHKLGFRGTTNTLL
                     NFGERGGAVGYLVGQPGDGLRCMFHMMNEARIGVGLGATMLGAAGYEASLAYAKQRSQ
                     GRPIGAGGKDASAPPVRLIAHADIRRMLLAQKAYVEGGMALALYCARLVDEQRTGTPE
                     SAAEASLLLEMLTPVAKSWPSEWALEANSLAIQVHGGYGYTRDFPVEQHWRDNRLNMI
                     HEGTHGIQALDLLGRKAVMQGGAGLKLLARRIDATIARALPQARHATHANALAQALQR
                     LGAATRSAWATASPEDALANATPYLQAFGHTIVAWLWLDIGAAIVDSGDAESTANVGR
                     LAALDYFMRYELPKTDAWLRVVEARDRTCRDVPEDAF"
     gene            6501..7331
                     /locus_tag="VIN75_03305"
     CDS             6501..7331
                     /locus_tag="VIN75_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973218.1"
                     /translation="MTEPSAIPTTAFAERRRIQSLFDLTGRTALVTGGSRGLGLQIAE
                     ALGEAGARVVLSARKSNELEAAVAALKERGVQADWIAADSSQPAEVQRLADEAIAKLG
                     HVDILVNNAGATWGAPAEDHPLDAWDKVMNLNVRSIFLLSQAVGKASMIPRKRGRIIN
                     VASIAGLSGNTGGMTTVAYNTSKAAVLNFTRALAGEWGVHGINVNALAPGFFPSRMTA
                     GLLAHVGVDNMRAHAPLRRLGDDEDLKGAALLLASDAGKHITGQLIAVDGGVSAVHGA
                     "
     gene            7339..7782
                     /locus_tag="VIN75_03310"
     CDS             7339..7782
                     /locus_tag="VIN75_03310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8973219.1"
                     /translation="MTDRTPLPFALDIPFVQALGMQLWRFDSGIAEISLDVRPEMQNS
                     WQVAHGGVVMTLLDVVMAHAARSVRDAGEHGTPGIVTLEMKTSFMRPATGRLVAWGHL
                     LHGTKGTAFCEGSVRDAGGALCAHATGTFKFLRKLKRGDELDAAS"
     gene            7840..8859
                     /locus_tag="VIN75_03315"
     CDS             7840..8859
                     /locus_tag="VIN75_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY8973220.1"
                     /translation="MSTINRQILLASRPTGEPVAENFKLVETPLAPLADGQVLVRHHF
                     LSLDPYMRGRMNDAKSYAAPQPLNEVMIGGTAGEVVESRNPHFKAGDKVVGMGGWQEY
                     AVVNADQRGVLQKVDTTHVPLSAYLGAVGMPGVTGWYGLVKIIAPKAGETVVVSSAAG
                     AVGGAVGQLAKARGCRVVGIAGGPDKCRMVKEELGFDECIDYRLYPDVKSMSGALKAA
                     CPGGIDGYFENVGGHLLDAVMLRSNAFARIAMCGMIAGYNGEPIPMTLPQLILTNRMK
                     VEGFIVSEHMEVWPEALKELGTLVATGKLKYKESVAQGLQNAPEAFFGLLKGRNQGKQ
                     LVKLV"
     gene            8883..9335
                     /locus_tag="VIN75_03320"
     CDS             8883..9335
                     /locus_tag="VIN75_03320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973221.1"
                     /translation="MLNWQWTRFEGLSGDDVYDMLALRARIFVLEQDCAYLDPDGLDR
                     QSWHLLGRGADGALLAYVRVCDPGVKFDPPSIGRVVVDASQRGTGLGHVLIAEALRRC
                     DATYPGQRNRIGAQAHLQDFYGRHGYVAVGETYMEDDILHIDMERPAS"
     gene            9332..11008
                     /locus_tag="VIN75_03325"
     CDS             9332..11008
                     /locus_tag="VIN75_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8973222.1"
                     /translation="MTIPMSHGATHAVLNQVPPYEDVNLYEANAALRDAVRFNQPSLA
                     ADGLSALGKVAGSAGMQRHARLANLHTPVLHAHDRFGHRIDEVEFHPSYHVLLGAALH
                     HRLHGSPWGAGGPSHVERAAGFMLFTELEPSVLCPVSMTYAVAPALRANPALQQAFSG
                     LLASPRYDARFQLATTKTAMTMGMGMTEKQGGSDVRANTTQAVRDGEDAWGERYRLVG
                     HKWFMSAPMSDAFLVVAQTAAGPTCFFLPRWLPDGTLNAVRIQRLKDKLGNKANASSE
                     VEFADAFAWRVGDEGRGLPQILAMGALTRLDCALGTAGLMRGAASLALHHATHRMAFG
                     KRLVDHALMRNVLADLALESEASTALALRLARAVDRSEDASAPAAEREHEALMRRLLT
                     PAAKYWVCKRGSHLAQEAMECLGGNGYVEEHGEGVMARIYREMPLNSIWEGAGNIMAL
                     DLLRALRSGPIADALAHETRAAKGAHPMLDRATQRLLARMENGVDEIDARRFARDLAL
                     VTQGALLAQTAPAPVFAAFCESRLDGAPDAFGQLGASTDFDAILRRAAAH"
     gene            11046..11957
                     /locus_tag="VIN75_03330"
     CDS             11046..11957
                     /locus_tag="VIN75_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973223.1"
                     /translation="MTTNHWQGRTAVITGAASGFGLETALLAARKGMNVVMADVMMEA
                     LGDAVGRVQALGAPVLSLRCDVRHASEIEAMGAATQARFGAPHLVFNNAGVGAGGLIW
                     EHTARDWDWVMGVNVMGVAHGVRVFTPMMLAAEKADPGYSGHIVNTASMAGLVNMPNM
                     GVYNVSKHAVVALSETLYQDLRLVSDRVHAHVLCPYFVPTGIAHSERARPQDAQEQGR
                     KATASQRVAQGLIEKAVASGRVTAADVAQFVFDALEADRFYIYSHPKALAPVTTRAEE
                     IVSATNPSDPYRERPELGEQLRAALRR"
BASE COUNT         1961 a         4305 c         4235 g         1643 t
ORIGIN      
        1 gcctgttccc ggtggcttac acgcccggca aggcaccggc ggccgcgccg ggcgcgccga
       61 cgccgccgga cgggctcgtc gcgcagcccg tcaccttcga cggccacgtc gaggcgccgc
      121 gcaccgagtg gttcatcgcg ggcaccgaga ccgcgcgcgt cgcgatggcg agcgcggccg
      181 gcagcgcggt gcgcctcatc gcctcgccgg acgacaagtc cgtcatcgcg ctcgaccccg
      241 acatgccgcc ggccgtgcag cgcctgcgct tcgaggccgt ggcgccggtg cccgcgggcg
      301 cgtggtggcg gctggacggc aagccgctgg gcgccgcgcg gccgctgccg tgggcgccgt
      361 ggccggggca tcacgaactg gcgctggtcg acgcgacggg caaggcgctg gacacggtca
      421 cgttcgacgt gcgcggcgcg caggcgaagc cgcccggtgc acccacacgc aagcctgcgc
      481 gcagcctcgg cgcggccgcc cgacccggcg cctgacgcgg tctccttcgc aggcgttccg
      541 gcgtcgcggg cgctgtgcga cgatcacgaa cgatctgtcg acgcacgccc ggcctgcggg
      601 ttttccatgg gatgtccgag tgctccgatc ccttgtcgca gccgggacag tcgcgcttcc
      661 gcgaacggtc ctagaatcga acgatcgttc tatttttcgg ttcacctcca ccatcgaacc
      721 gcaaaccgga gacctgcccc atgagtgcga gttacgaagt ccgcggcgcc gtcgccgtca
      781 tcacgctgaa caacccgccg gtcaacggcc tgggccacga gacgcgcaag gcggtcgccg
      841 aaggcatcga gaaggcggag gacgatgccg cggtcaaggc gatcgtcatc accggcgccg
      901 gcaaggcgtt ctcgggcggc gccgacatcc gcgagttcgg ttcgcccaag gcgctggccg
      961 agcccaacct gctgagcctg atcgcacgcg tcgaaggcgc gtccaagccg gtggtggccg
     1021 cggtgcacac cgtgtgcatg ggcggcggcc tcgagctggc gctcggttgc cattaccgcg
     1081 tcgccagttc cggcgccagc atcgcgctgc ccgaggtcaa gctgggcatc ctgccgggcg
     1141 ccggcggcac gcagcgcctg ccgcgcgcga tcggcgtcga gccggcgctc aacatgatcg
     1201 tcagcggcga gccggtcaag agcgagcacc tcgcgctgat ccccggccag aagctgttcg
     1261 accgcgtgta cgagcgcgac ttcatggaca gcgccatcgc gttcgcgagc gagatcgccg
     1321 cggtgcgccc gctgccgatg atccgcgaca tcaagtgcaa gcatccggaa ggcgacgcct
     1381 acttcggctt cgcgcgcaac acggccaagg cgatggccaa gaacttcccc gcgccggcgc
     1441 gctgcgtcga ctgcgtcgag caggccacca aggccaagtt cgaggacggc ctgaagtacg
     1501 agcgcgaggc gttcgccgcg ctgatgctca cgcccgagag caaggcgctg cgccacgcgt
     1561 tcttcgccga gcgcgcggcg tccaaggtgc ccgacatccc cgaggacacg cccgtgcgtc
     1621 cggtcgccaa ggtcgcgatc atcggcgcgg gcaccatggg cggcggcatc gcgatgaact
     1681 tcctcaacgc gggcatcccc gtggtcatgc tcgagatgaa gcaggaggcg ctggacaagg
     1741 gcctggccat catccgcaag aactacgagg cgcaggtcaa gaagggcaag ctcaaggagg
     1801 acaagtacgc cgaacggatg gcgctgctgt cgaccacgct gtcctatgac gacctgcgcg
     1861 acgccgacct cgtcatcgag gcggtgttcg aggagatcgg cgtcaagcag aaggtgttcg
     1921 agcagctcga cgccgtcatg aagccgggcg cgatcctcgc gtccaacacg tccacgctgg
     1981 acctggacaa gatcgcgtcg ttcacgaagc gcccgcagga cgtgatcggc atgcacttct
     2041 tcagcccggc caacgtgatg aagctgctgg aagtcgtgcg cggcgccagg acggcgcccg
     2101 acgtgctgat gacggtgatg aagctgggca aggtgatccg caagacctgc gtggtctcgg
     2161 gcgtgtgcga cggcttcatc ggcaaccgca tgatcgagca gtacagccgc caggccggct
     2221 tcctgctcga ggagggctgc acgccgcagc aggtggacaa ggcgatcgag aagttcggct
     2281 tcgcgatggg cccgttccgc atgggcgacc tggccggcaa cgacatcggc tgggcgatcc
     2341 gcaagcgccg ctacgtcgag aagcccgggc agcgctactc gaagacggcc gacctgctgt
     2401 gcgagctggg ccgcttcggc cagaagaccg gcgccggctg gtacgactac gtgccgggca
     2461 agcgcgacgc gatcccgtcc aagctcgtgg aagacatgct ggtcaagcat cgcgaggaac
     2521 tgggcatcgc gccgcgcaag atcagcgacg acgagatcgt gcaccgcctc gtctacgcgc
     2581 tggtcaatga aggcgcccac atcgtcgagg aaggcatcgc gctgcgcgcg tccgacatcg
     2641 acatggtcta cctgaccggg tatggcttcc cactgtggcg cggcggcccg atgaactacg
     2701 ccgacaccgt gggcctgtgg aacgtggcgc aggcgatgaa gaagttcgcg aagaacccgg
     2761 ccgacgacca ggcgttctgg cagcccgcgc cgctgctggc ccgcctcgcc gccgaaggca
     2821 agtccttcag ctgaggacgc gggcgatgcc gggttccgtc aagcgcgcgg cggccgtcgt
     2881 ggccggcgtc gtgctgggaa tggcgatcgc cgcgggcgcg agtccgtggc gcgagctgtt
     2941 caagccaagc gaacccgtgc agcccgcccc catccagcgc gagcgcgccc ccgacacgcg
     3001 cgtctgacct tttcgaaacg aacgtttcca gaatatccag gagttccgac atgaccgaag
     3061 ccgtcatcgt ttccaccgcc cgcacgccgc tctgcaagag ctggaagggt gccttcaaca
     3121 tgacgcacgg cgccgagctg ggcggccacg ccgtcaaggc ggctctcgag cgcgccggca
     3181 tcgacggcgc cgaagtcgag gacgtgctca tgggctgcgc caaccccgaa ggcgcgaccg
     3241 gcgccaacat cgcccgccag gtcgcgctgc gcgccggcct gccggtcagc gtggccggcg
     3301 tcaccgtcaa ccgcttctgc tccagcggcc tgcagacgat cgcgatggcc gcgcagcgca
     3361 tcatcgccaa cgaaggcgac gtgtacgtgg ccggcggcgt ggagagcatc tcgtgcgtgc
     3421 agcaggagat gaaccaccac atgctgcgcg acatgtggct ggcgcggaac aagcccgcgg
     3481 tctacatgtc gatgctcgag accgccgaga cggtggccaa gcgctacaag atctcgcgcg
     3541 agcgcatgga ccagtacggc gcggccagcc agcagaaggc caccgcggcg ctcgagaagg
     3601 gcctgttcaa ggacgagatc gcgcccatca ccgtcaccgc cggcgtcgcc gatccgaaga
     3661 acggcatccg caccagggaa gtgactgtcg agaacgacga gggcatccgc ccgggcacga
     3721 cctacgacgg catcaaggac atccgcccgg cggtgcccgg cggcgtgatc agcgccggca
     3781 acgccagcca gttcagcgac ggcggcggcg cctgcgtggt cgtctccagc cactacgccg
     3841 agcgcaaggg cctcaagccc ctgggccgct tcctcggctt cgccgtggcc ggctgcgagc
     3901 ccgacgagat gggcatcggc ccggtctatg ccgtgcccaa ggtgctcaag cggctgggcc
     3961 tgacggtggc cgacatcgac ctgtgggaac tgaacgaggc gttcgcggtg caggtgctgt
     4021 actgcgccga caccctgggc atcccgatgg atcgcctgaa cgtcaacggc ggcgccatcg
     4081 ccgtcggcca cccgtacggc gtcagcggcc agcgcctgac cggccacgcg ctgatcgaag
     4141 gcaagcgccg cggcgcgaag aaggtctgcg tgacgatgtg catcggcggc ggcatgggcg
     4201 cggccggcgt cttcgaagtc ctgtaagcga cgccttccga gcgagggagc gcgacgtgat
     4261 cgatctcgat tccgttcgaa gcttcttcgc ccgggcgccg ttcatggtcg atctgggcgt
     4321 cgtccccacc gccgtatcgg agggccgctg cgagaccgag ctggtgctgc aacagcggca
     4381 cctgcagcac acgggccagg cgcacgccgg cgtcgtcacc gcgatggccg accacaccgc
     4441 gggcgccgcg gcgcaaagcg tgatcaagga cggcggcgtc gcgatcaccg ccgaactcaa
     4501 gaccagcctg ctgcggccgg cggtcggcag ccggctggtg tgcgtcggca acgtgatcaa
     4561 ggccggacgc acgctgatct tcgcggaatc ggacgtcttc gccatcctcg gcgagcagcg
     4621 cacgctggtc gccaaactga gcgccacgct ggccgtggtg cagccgacat gagcactcgc
     4681 caatccctcg attttctcct gtacgactgg ctgaagatcg acgatctcac gacgcgcgaa
     4741 cgcttcgcgg atcattcgcg cgagaccttc gacgcggtgc tcgacaccgt cgagcgcctg
     4801 gcgcgcgaga agttcgcgcc gttcaaccgc ctcgtcgaca ccgaggagcc aagggtcgac
     4861 ggcgagcgcg tgcgcctgcc gcaggccacg cacgacgcgc tgcgcgccta cgcggacacc
     4921 ggcatgatcg ccgcgtcgca ggacgccggc atcggcggca tgcagctgcc ctgcaccgtg
     4981 gagaaggcgg ccaacgcctt tctctcgcag gcctgcatcg ggatgggcgg ctacgtgatg
     5041 ctcacgcacg gcaacgcggc gctgctgatg gcgcacggca cgccccggca acgcgacgtc
     5101 ttcgcggcgc gcgagttctc gggcgagtgg ttcggcacga tgtgcctcag cgagccgcag
     5161 gccggctcgt cgctgagcga catcgccacg cgcgccgtgc ccgacggcgc cgacttcgaa
     5221 accgacccgc tgggtccgcg ctacaagctg gtgggcaaca agatgtggat ctcggccggc
     5281 gagcacgaga tcacgcccaa catcgtgcac ctggtgctgg ccaagatccc cgggccggac
     5341 ggcaagctgc cggcgggcgt caagggcatc tcgctgttca tcgtgcccaa gcaccgcgtg
     5401 gacgccgagg gccggctgac ggggcagcgc aacgacgtca gcctggccgg cctgaaccac
     5461 aagctgggct tccgcggcac caccaacacg ctgctgaact tcggcgagag gggcggggcg
     5521 gtcggatacc tggtcgggca accgggcgac ggcttgcgct gcatgttcca catgatgaac
     5581 gaggcacgca tcggcgtcgg gctcggcgcc accatgctgg gcgccgccgg ttacgaggcg
     5641 agcctcgcgt acgcgaagca gcgctcgcag gggcgcccga tcggcgcggg cggcaaggat
     5701 gcatcggcgc cgccggtgcg cctcatagcg cacgccgaca tccgccgcat gctgctcgcg
     5761 cagaaggcgt acgtcgaggg cggcatggcg ctggcgctgt actgcgcgcg gctggtcgac
     5821 gagcagcgca ccggcacgcc ggagtccgcg gccgaggcga gcctgctgct ggagatgctc
     5881 acgccggtcg ccaagagctg gcccagcgaa tgggcgctgg aggccaacag cctggcgatc
     5941 caggtccacg gcggctacgg ctacacgcgc gactttcccg tcgagcagca ctggcgcgac
     6001 aaccgcctga acatgatcca cgagggcacg cacggcatcc aggcgctcga cctgctgggc
     6061 cgcaaggccg tgatgcaggg cggcgcgggc ctgaagctgc tggcccgtcg catcgacgcg
     6121 accatcgcgc gcgcgctgcc gcaggcgcgt cacgcgacgc acgcgaacgc gctcgcgcag
     6181 gcgctgcagc gcctcggcgc ggccacgcga tcggcctggg ccaccgcctc gccggaggac
     6241 gcgctcgcca acgcgacgcc ctacctgcag gcgttcggcc acacgatcgt cgcgtggctg
     6301 tggctggaca tcggcgccgc gatcgtcgac agcggcgacg ccgagtcgac cgccaatgtc
     6361 ggccgcctgg ccgcgctcga ctacttcatg cgctacgagc tgcccaagac cgacgcctgg
     6421 ctgcgcgtcg tcgaggcgcg cgaccgcacc tgccgcgacg tccccgagga cgccttctga
     6481 ttccaacgac acgagacacg atgaccgaac cctccgccat tcccaccacc gcgttcgccg
     6541 agcgccgccg catccagtcg ctgttcgacc tgacggggcg cacggcgctg gtcaccggcg
     6601 gctcgcgcgg gctgggcctg cagatcgccg aggcgctcgg cgaggccggc gcgcgcgtcg
     6661 tgctcagcgc gcgcaagagc aacgagctcg aggccgccgt cgcggcgctg aaggagcgcg
     6721 gcgtgcaggc cgactggatc gccgccgatt ccagccagcc cgccgaggtg cagcgcctgg
     6781 ccgacgaggc catcgcgaag ctggggcatg tcgacatcct ggtcaacaac gccggcgcca
     6841 cctggggcgc gccggcagag gatcatccgc tcgacgcgtg ggacaaggtg atgaacctga
     6901 acgtccgctc gatcttcctg ctctcgcagg cggtgggcaa ggcgagcatg atcccgcgca
     6961 agcgcggccg catcatcaac gtggcgtcga tcgcgggcct gtcgggcaac acgggcggca
     7021 tgaccaccgt ggcctacaac acgagcaagg ccgcggtgct gaacttcacg cgcgcgctgg
     7081 ccggcgagtg gggcgtgcac ggcatcaacg tcaacgcgct ggcgccgggc ttcttcccga
     7141 gcaggatgac ggccggcctg ctcgcgcatg tcggcgtcga caacatgcgc gcccacgcgc
     7201 cgctgcgccg actcggcgac gacgaggacc tgaagggcgc cgcgctgctg ctggccagcg
     7261 acgcgggcaa gcacatcacc ggccagctga tcgccgtcga cggcggcgtc agcgcggtgc
     7321 acggcgcctg aggacgccat gaccgaccgc acgccgctgc ccttcgcact ggacatcccg
     7381 ttcgtccagg cgctgggcat gcagctctgg cgcttcgatt cgggcatcgc cgagatcagc
     7441 ctggacgtcc gccccgagat gcagaactcg tggcaggtgg cgcacggcgg cgtggtcatg
     7501 acgctgctgg acgtggtcat ggcccatgcc gcgcgcagcg tgcgcgacgc cggggagcac
     7561 ggcacgcccg gcatcgtcac cctcgagatg aagaccagct tcatgcggcc cgcgaccggc
     7621 cggctggtcg cctggggcca cctgctgcac ggcacgaagg gcaccgcgtt ctgcgagggc
     7681 agcgtgcgcg acgccggtgg cgcgctgtgc gcccacgcca cgggcacctt caagttcctg
     7741 cgcaagttga aacgcggcga cgagctcgac gccgcatcct gaaccaccgg cggtcggcac
     7801 cccccgatgc cgagccgaca ccaccacgga gactccacca tgtccacgat caaccgccag
     7861 atcctgctcg cctcgcgtcc caccggcgag cccgtcgccg agaacttcaa gcttgtcgag
     7921 acgcccctcg cgccgctggc cgacggccag gtgctggtgc gccatcactt cctgagcctc
     7981 gatccgtaca tgcgcggccg catgaacgac gccaagagct acgccgcgcc gcagccgctg
     8041 aacgaagtga tgatcggcgg caccgccggc gaggtcgtgg aatcgaggaa cccgcacttc
     8101 aaggccggcg acaaggtcgt gggcatgggc ggctggcagg agtacgcggt ggtcaacgcg
     8161 gaccagcgcg gcgtgctgca gaaggtcgac acgacgcacg tgccgctgtc ggcctacctg
     8221 ggcgccgtgg gcatgcccgg cgtcacgggc tggtacggcc tcgtgaagat catcgcgccg
     8281 aaggcgggcg agacggtcgt ggtcagctcc gcggccggcg cggtcggcgg cgcggtgggc
     8341 cagctcgcca aggcgcgcgg ctgccgcgtg gtgggcatcg ccggcggccc ggacaagtgc
     8401 cgcatggtca aggaggagct cggcttcgac gagtgcatcg actaccgcct gtaccccgac
     8461 gtgaagtcga tgagcggcgc gctcaaggcc gcctgcccgg gcggcatcga cggctacttc
     8521 gagaacgtcg gcggccacct gctggacgcc gtgatgctgc gttcgaacgc gttcgcgcgc
     8581 atcgccatgt gcggcatgat cgccggctac aacggcgagc cgatcccgat gacgctgccg
     8641 cagctgatcc tgaccaaccg catgaaggtc gagggcttca tcgtcagcga gcacatggag
     8701 gtctggcccg aggcgctgaa ggaactcggc acgctggtgg ccaccggcaa gctcaagtac
     8761 aaggaatcgg tggcgcaggg cctgcagaat gcacccgagg cgttcttcgg cctcctgaaa
     8821 ggccggaacc agggcaagca gctggtaaaa ctcgtctgag acgaaccaac cggacgagag
     8881 cgatgctgaa ctggcaatgg acccgcttcg agggactgag cggcgacgac gtgtacgaca
     8941 tgctcgccct gcgcgcgcgc atcttcgtgc tggagcagga ctgcgcctat ctcgatccgg
     9001 acggcctcga tcggcagtcg tggcacctgc tgggccgcgg tgccgatggg gcgctgctcg
     9061 cctacgtgcg cgtgtgcgac cccggcgtga agttcgaccc gccgtcgatc ggccgcgtgg
     9121 tggtcgacgc gtcgcagcgc ggcaccgggc tgggccacgt gctgatcgcc gaggcgctgc
     9181 gccgctgcga cgccacgtac ccgggccagc gcaaccgcat cggcgcgcag gcccacctgc
     9241 aggacttcta cggccgccac ggctacgtgg cggtcggcga gacctacatg gaggacgaca
     9301 tcctccacat cgacatggag cggcccgcat catgacgatc ccgatgtccc acggcgcgac
     9361 gcacgccgtg ctcaaccagg tgcctccgta cgaggacgtc aacctgtacg aggcgaacgc
     9421 cgcgctgcgc gatgcggtgc ggttcaacca gccgtcgctg gcggccgatg ggttgtcggc
     9481 gctcggcaag gtggccggca gcgccggcat gcagcgccac gcgcgccttg cgaacctgca
     9541 cacgcccgtg ctgcacgcgc acgaccgctt cggccaccgc atcgacgagg tcgagttcca
     9601 tcccagctac cacgtgctgc tgggcgcggc gctgcaccac aggctgcacg gctcgccgtg
     9661 gggtgccggc gggccgagcc acgtcgagcg cgcggccggc ttcatgctgt tcaccgagct
     9721 cgaaccctcg gtgctgtgcc ccgtgtcgat gacctacgcg gtggcgcccg cgctgcgcgc
     9781 caacccggcg ctgcagcagg cgttctcggg cctgctggcc agcccccgct acgacgcccg
     9841 cttccagctc gccacgacca agacggcgat gacgatgggc atgggcatga ccgagaagca
     9901 gggcggctcc gacgtgcgcg ccaacacgac gcaggccgtg cgcgacggcg aggacgcctg
     9961 gggcgagcgc tatcgcctcg tcggccacaa gtggttcatg tcggcgccga tgagcgacgc
    10021 cttcctggtc gtcgcgcaga cggccgccgg cccgacctgc ttcttcctgc cgcgctggtt
    10081 gccggacggc acgctcaacg cggtgcgcat ccagcgcctg aaggacaagc tgggcaacaa
    10141 ggccaacgcg agctcggagg tggagttcgc cgacgcgttc gcgtggcgcg tcggcgacga
    10201 gggccgcggc ctgccgcaga tcctcgcgat gggcgcgctg acgcgcctcg attgcgcgct
    10261 cggcaccgcc ggtctcatgc gcggggccgc atcgctcgcg ctgcaccacg cgacgcaccg
    10321 catggccttc ggcaagcgcc tggtcgacca cgcgctgatg cgcaacgtgc tggccgacct
    10381 cgcgctcgag agcgaggcct cgaccgcgct cgcgctgcgc ctggcgcgcg ccgtcgatcg
    10441 cagcgaggac gcgtcggcgc ccgccgccga gcgcgagcac gaggcgctga tgcggcgcct
    10501 gctgacaccc gcggccaagt actgggtctg caagcgcggc agccacctcg cccaggaggc
    10561 gatggaatgc ctgggcggca acggctacgt cgaggagcac ggcgagggcg tgatggcacg
    10621 catctaccgc gagatgccgc tcaattccat ctgggaaggc gcgggcaaca tcatggcgct
    10681 ggacctgctg cgcgcgctgc gctccggtcc gatcgccgac gcgctcgcgc acgagacgcg
    10741 cgccgccaag ggcgcgcacc cgatgctcga tcgcgccacg cagcggctgc tggcgcgcat
    10801 ggagaacggc gtcgacgaga tcgacgcgcg ccgcttcgcg cgcgatctcg cgctcgtcac
    10861 gcagggcgcg ctgctcgcgc agacggcgcc cgcgccggtg ttcgcggcgt tctgcgagag
    10921 ccgcctcgac ggcgcgcccg acgcgttcgg ccagctcggc gcctccaccg atttcgacgc
    10981 catcctccgg cgcgccgcgg cgcactgatt ccaacaccga caccaaccag ggcacgagac
    11041 accccatgac caccaaccac tggcaaggcc gcacggccgt catcaccggc gcggcctccg
    11101 gcttcggact cgagactgcg ctgctggccg cgcgcaaggg catgaacgtc gtgatggccg
    11161 acgtgatgat ggaggcgctc ggcgacgccg tcggccgggt gcaggcgctc ggcgcgccgg
    11221 tgctgtcgct gcgctgcgat gtgcgccatg catccgagat cgaggccatg ggcgccgcca
    11281 cgcaggcccg cttcggcgcg ccgcacctcg tgttcaacaa cgcgggcgtg ggcgccggcg
    11341 gcctcatctg ggagcacacc gcgcgcgact gggactgggt gatgggcgtc aacgtgatgg
    11401 gcgtggcgca tggcgtgcgc gtgttcacgc cgatgatgct ggccgccgag aaggccgacc
    11461 ccggctactc gggccacatc gtcaacaccg cgtcgatggc aggcctggtc aacatgccca
    11521 acatgggcgt ctacaacgtc agcaagcatg cggtcgtcgc gctcagcgag acgctctacc
    11581 aggacctgcg cctcgtgagc gaccgcgtgc acgcgcacgt gctgtgcccg tacttcgtgc
    11641 ccaccggcat cgcgcacagc gagcgcgccc gcccgcagga cgcgcaggag cagggccgca
    11701 aggcgaccgc cagccagcgc gtcgcccagg gcctgatcga gaaggccgtc gcgagcggcc
    11761 gcgtgaccgc cgccgacgtc gcccagttcg tgttcgacgc gctcgaggcc gaccgcttct
    11821 acatctacag ccacccgaag gcgctcgcgc cggtgacgac gcgggccgag gagatcgtct
    11881 cggcgacgaa cccgtcggac ccgtatcgcg agcgtcccga actgggcgag caactgcggg
    11941 ccgcattgcg ccgctgacgc ggctttcagg gttcgtccgg agcgaagcag cgccagagtg
    12001 cccatcccct tcatgtatca tggcggggta ggcaatacat aagaccatcg tccagaagga
    12061 ctcctccggc tcccgtcgtg tgaacgatgt ttcggccagg tcgaactctc cagtttgatt
    12121 ttcttgcgta gtacctgccc tgcg
//
LOCUS       DATCRO010000122         9694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_158897, whole genome shotgun sequence.
ACCESSION   DATCRO010000122 DATCRO010000000
VERSION     DATCRO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158897"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIN75_03335"
     CDS             <1..193
                     /locus_tag="VIN75_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8973224.1"
                     /translation="AMTIDKIAGPVGEALIFTAAGLAVAIPAVLAYNIFGKRVAACEA
                     ELEGFAHDLREMQLDAAAR"
     gene            204..620
                     /locus_tag="VIN75_03340"
     CDS             204..620
                     /locus_tag="VIN75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8973225.1"
                     /translation="MAFGRLERSPTPQPMSDINMTPLIDVMLVLLVIFMIAAPLMTSS
                     LKMDLPKVAAATSNDAEAALSLGITADGRLHVGDQVVTHDELVRALKQTAARQPQPDV
                     LLRADQSVPYGRVAELIGLVQDAGLTHIGFIAQPAK"
     gene            661..1800
                     /locus_tag="VIN75_03345"
     CDS             661..1800
                     /locus_tag="VIN75_03345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973226.1"
                     /translation="MSALATFQARLRATFALPDMSADERAAFRQAQADQRDALVVPVV
                     VAAVAMIAGFVGWDQLRDPGHLGPAAAVRLGGSALVLALLAAVRQVRGLPFRIQALVM
                     YCAIYALQLAIGVALGHEAPLQMPGLLIVMFFGVIGLPRASDSLVNIALAAVSMPLVL
                     PRRPLAADVVYALAHFATVVVLVWAACALLERMSAQSFAYRRRLQREASTDALTGLHN
                     RREFEAGMVREMERARRLRVPLTLALVDIDLFKRVNDRYGHDIGDRVLREVAATLKAG
                     VRRSDLVARIGGEEFALVLLGTEPPGASALLERLRQAVAALRVPAGADTIACTISAGI
                     TDQVEDDTDWAALYKRADQALYAAKQGGRDRIVEATPPEPEPALV"
     gene            complement(1862..2704)
                     /locus_tag="VIN75_03350"
     CDS             complement(1862..2704)
                     /locus_tag="VIN75_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973227.1"
                     /translation="MKSLGIAAARAACVVALVASASASRAATEHEQEVGAQVEQLCDA
                     RALAALGRHVNVANFKEPALGHDADAGAMVAAAACRGVPASHGRLVAAAYRSGHQDDL
                     LLVIALLDGSGNVVSSFKGALEADPALKVVSGSLWLDTAAYDIAPGVRAFGLDVTSGA
                     PRGCAGMGSGARRSLFVSQGRFIRPVLQDLPMSEWALIQRGKSACTDSGAPELTITEN
                     FTAKLALAPTSTKGWRDLVVTGTASRDDGHLDERPPFSAVLKWDGRMYSIDPQQQAWA
                     AWRQ"
     gene            2935..5577
                     /gene="leuS"
                     /locus_tag="VIN75_03355"
     CDS             2935..5577
                     /gene="leuS"
                     /locus_tag="VIN75_03355"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8973228.1"
                     /translation="MNEKYVPSEVEAAAQAHWQATDAHRVTEDASRAKFYACSMLPYP
                     SGKLHMGHVRNYTINDMLARQLRMKGMNVLMPMGWDAFGLPAENAAIKNKVPPARWTY
                     DNIAHMKEQMKAMGLAIDWSREVATCDPTYYKWNQWLFLKMLEAGICERRTQIVNWDP
                     VDMTVLANEQVIDGHGWRSGAPVEKREIPGYYLNIVKYAEELLSAVADPADPEYLSGW
                     PERVRLMQENWIGKSEGVRFAFPHTIHDDDGKLIGDGKLYVFTTRADTIMGVTFCAVA
                     PEHPLATHAAKGNPQLAAFIETCKAGGTTEAELATKEKEGLPTGLTVIHPLTGAQVPV
                     WVGNYVLMTYGDGAVMGVPAHDERDFAFARKYGLAIKQVVAVAGETFSTDAWQEWYGD
                     KQRGTCIDSGVLDGLGMKDAVNRVAELVGAAGLGEKKTTWRLRDWGISRQRYWGTPIP
                     IIHCDDCGVVPVPEKDLPVVLPEDLIPDGSGNPLNKHAAFLNVGCPKCGKPARRETDT
                     MDTFVDSSWYFMRYCDPSNSEKMVADGTDYWMPMDQYIGGIEHAILHLLYARFWTKVM
                     RDLKLVKFSEPFKNLLTQGMVLKGAFFHKPEDAGKNYYWEHELDVERDAHGNVVSAKL
                     KDGTPVTYEMTTMSKSKNNGVDPQALIDRYGADTARLFVMFASPPEQTLEWNDSGVEG
                     AHRFLKRVWGYGVKNADALRGAADGVAGLELAAPAKALRREVHVLLKQVTYDYERMQY
                     NTVVSGAMKLLNALEGFALDGSAGSAAVAREGFSVLLRSLYPACPHIAQALWTDIGYG
                     QGKELVDAGWPAVDEAALVQDEIELVLQVLGKTRGSVLVPASADKAAIEALALASPEA
                     VRWMEGKPARKVVIVPGRLVNIVV"
     gene            5585..6214
                     /gene="lptE"
                     /locus_tag="VIN75_03360"
     CDS             5585..6214
                     /gene="lptE"
                     /locus_tag="VIN75_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY8973229.1"
                     /translation="MRAQSSVRRRVALAVLAVSAAATLAACGFHRRIAQPLNYERIAL
                     SGFADRSTMAEEIRRALPSTARIVSNVLESQVVIEAVEDTQKTTVEASTAFGQVRELE
                     LHCKLRYRVLDPNGTELLPLADIERFRDLTYDEKDALAKDTEQKALYRDMQSDMAYQL
                     IRVLSAVGKPAGPAAQAQAARAASAAVAPASAPSSVSAVEWAASQAAGR"
     gene            6225..7301
                     /gene="holA"
                     /locus_tag="VIN75_03365"
     CDS             6225..7301
                     /gene="holA"
                     /locus_tag="VIN75_03365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8973230.1"
                     /translation="MQVRPEQLPDHLARGLRDVYVVHGDEPLRAQEAADAIRAAVRAG
                     GASERKVFVVSGAHFDWSGVIGASQAMGLFADQQLIEIRIPSGKPGKDGSEALQELAA
                     SAGPDSRLLVHLPKLDWQQQKAAWFTTLDAAGVSVAADIVERNALPAWLAKRFAAQGQ
                     RVEAGAAGERTLAFMADRVEGNLLAAHQEIQKLGLLYPPGELTFAQVEDAVLDVARHD
                     VSRLCDAVMAVQVPRALRMLEGLQAEGEAAVFVHYKLSDDIRAMKRIQDAMAEGKPMP
                     MALREARIWGARERLFERALPRLSAPMAARLVSAASECDGVLKGLRHPQWPSEPWDAL
                     RRLVLMMLQALRPEGAQAGLVLRT"
     gene            7343..7768
                     /locus_tag="VIN75_03370"
     CDS             7343..7768
                     /locus_tag="VIN75_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973231.1"
                     /translation="MLHRRLLALLVTSATLVAGCASRVEKGAAAPIAPLDNSDRASAA
                     PSDRAEVAGLPDWLRIRLADYDAQSGPAAPRAVYEVRYGDGVAYYVQAGCCDQLDPLV
                     DAHGVLVCYPSGGFTGRGDGKCLGALAPVAERREVWRHR"
     gene            complement(8179..9126)
                     /locus_tag="VIN75_03375"
     CDS             complement(8179..9126)
                     /locus_tag="VIN75_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY8973232.1"
                     /translation="MRIAFDARMDGAERADWWRALTDALPDDEWIDPVVDGTNSLRWR
                     EADVAVVANPLPGRLANCTQLRLIQSLWAGVDRLLADSTLPRDVPIARMVDPMMNRAM
                     AETATWAVLSLHRRAFDYAAQQRQTQWRPLPQKRADEVAVLVLGLGQMGRTAALQLAA
                     LGYRVTGWSARAQQLDGMATSAGWAALPAALGAADIVVNLLPLTEDTRGLFDRTRLAQ
                     MRPDASLVNLARGGHVVEKDLLEALNAGRLAHAVLDVFDTEPLPATHVFWFHPRITVW
                     PHAAAQTDLRSAAAVVAANVEAVRRGESAAHRVDRTRGY"
     gene            complement(9123..9683)
                     /locus_tag="VIN75_03380"
     CDS             complement(9123..9683)
                     /locus_tag="VIN75_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8973233.1"
                     /translation="MTTNRGISIDDAFVARVEKLGDALRARNWRLATAESCTGGLLAG
                     ACTAPAGASDWFAAGFVSYANDAKTGLLGVPAPLIARHGAVSAEVADAMARGALARAD
                     VQLALSITGIAGPGGGSAAKPVGTVWLGVAWLHPRETQEGLPAGGDAPLVGSRTERLQ
                     LDGDRAAIRAQTVAIALERLIAAARA"
BASE COUNT         1413 a         3518 c         3444 g         1319 t
ORIGIN      
        1 cgccatgacc atcgacaaga tcgccggccc cgtcggcgag gcgctgatct tcacggccgc
       61 cggcctggcg gtcgcgatcc ccgccgtcct ggcctacaac atcttcggca agcgcgtggc
      121 ggcctgcgag gccgagctcg aaggcttcgc gcacgacctg cgcgagatgc agctggacgc
      181 cgccgcgcgc tgagggaacc gccatggcct tcggtcgact ggagcgcagc cccaccccgc
      241 agccgatgag cgacatcaac atgacgccgc tcatcgacgt catgctggtg ctgctggtga
      301 tcttcatgat cgccgcgccg ttgatgacct ccagcctcaa gatggacctg cccaaggtcg
      361 ctgccgccac cagcaacgac gccgaagccg ccctgtcgct gggcatcacg gccgacggtc
      421 gcctgcacgt cggcgaccag gtcgtcacgc acgacgagct ggtgcgcgcg ctcaagcaga
      481 ccgccgcgcg ccagccgcag cccgacgtgc tgctgcgtgc cgaccagagc gtgccctacg
      541 gccgcgtggc cgagctcatc ggcctggtgc aggacgccgg gctgacgcac atcgggttca
      601 tcgcgcagcc cgcgaaatga cctcgcccat ggccgggctc gaaatctgcc cgactgtgga
      661 atgagcgccc tcgccacctt ccaggcccgg ctacgcgcga cgttcgcgct gccggacatg
      721 agcgccgacg agcgcgcggc gttccgccag gcacaggctg accagcgcga cgccctcgtc
      781 gtgcccgtcg tggtggccgc cgtggcgatg atcgcgggtt tcgtggggtg ggaccagctg
      841 cgcgatccgg gtcacctcgg cccggctgcg gcggtgcgcc tgggtggcag cgccctcgtg
      901 ctggccctgt tggccgcagt gcgccaggtg cgcggccttc cgttccggat ccaggccctc
      961 gtcatgtact gcgcgatcta cgcgctgcag cttgccatcg gcgtggcgct gggccacgag
     1021 gcgccgctgc agatgcccgg cctgctgatc gtgatgttct tcggcgtgat cggcctgccg
     1081 cgcgcgagcg actcgctggt caacatcgcg ctcgccgcgg tcagcatgcc gctggtgttg
     1141 ccacgccggc cgctggcagc cgatgtcgtg tatgcgctgg cgcatttcgc caccgtcgtc
     1201 gtgctggtct gggcggcgtg cgcgctgctg gaacgcatgt ccgcgcagag cttcgcctac
     1261 cgtcgccgcc tgcagcgcga ggccagcacc gacgcgctga ccggcctgca caaccggcgc
     1321 gaattcgagg ccggcatggt gcgcgagatg gagcgcgcgc ggcgcctgcg cgtgccgctg
     1381 acgctggcgc tcgtcgacat cgacttgttc aagcgcgtca atgaccgcta cggccacgac
     1441 atcggcgatc gcgtgctgcg cgaagtcgcg gcgacgctga aggcgggcgt ccgccgcagc
     1501 gacctcgtgg cgcgcatcgg cggcgaggaa ttcgcgctcg tgcttctggg caccgagcca
     1561 cccggagcgt cggcgttgct ggaacggttg cggcaggcgg tggcggcgct gcgcgtgccc
     1621 gcgggcgcgg acaccatcgc ctgcaccatc agcgcaggca tcaccgacca ggtcgaggac
     1681 gacacggact gggcggcgct gtacaagcgc gccgaccagg ccctttacgc tgccaagcag
     1741 ggcggccgcg accgcatcgt ggaggcaacc ccgcccgagc ccgagcccgc gctcgtgtga
     1801 cgcgggcgcg cggcgcctcg cgcgtcatgc gccacggcct tcgcgcgcga agccgatggc
     1861 atcactgccg ccaggccgcc cacgcctgct gctgcgggtc gatcgagtac atgcggccat
     1921 cccacttcag caccgcgctg aagggcggac gctcgtcgag gtgcccgtcg tcgcgcgagg
     1981 ccgtgccggt gacgaccagg tcgcgccagc ccttcgtgga cgtcggcgcc agggccagct
     2041 tcgcggtgaa gttctcggtg atcgtgagct cgggcgcgcc cgagtcggtg caggccgact
     2101 tgccgcgctg gatcagcgcc cattcggaca tgggcaggtc ctggagcacc gggcggatga
     2161 agcggccctg gctgacgaac aggctgcggc gcgcgcccga gcccatgccc gcgcagccgc
     2221 gcggcgcgcc gctggtcacg tccaggccga aggcgcgcac gccgggggcg atgtcgtagg
     2281 cggcggtgtc cagccacagg ctgccggaga cgaccttgag cgccggatcg gcctccagcg
     2341 cccccttgaa gctcgagacg acgttgcccg atccgtcgag cagcgcgatc accagcagca
     2401 gatcgtcctg gtggcccgag cgataggccg cggcgaccag gcggccgtgc gacgccggca
     2461 cgccccggca ggcggcggcg gcgaccatcg cgcccgcgtc ggcgtcatga ccgagcgccg
     2521 gctccttgaa gttcgccacg ttcacatggc gcccgagcgc ggccagcgca cgcgcgtcgc
     2581 acagctgctc gacctgcgcg ccgacctcct gctcgtgctc ggtggccgcc cggctggcgg
     2641 acgccgacgc gaccagcgcg acgacacagg ccgcgcgcgc ggcggcgatt cccaacgact
     2701 tcatttgtcc aactcccggt gcacggcccg ggacggtcca acgcgccctt ctcgccgtga
     2761 gaggcgcgat catcccggag gccgcgctgc tcgtccatgg gagcgcggta acgatccgtc
     2821 aaggcgtgcg acagggcgtc acctgcgccg ccggccgccg ccgcggcgcg aaaccggccg
     2881 ggaatgtcgt cccgcgcgcc gggcgcgggc caccgcccct cctacaatcg gggaatgaac
     2941 gaaaagtacg tcccctccga ggtcgaggcc gccgcccagg cgcactggca agccaccgat
     3001 gcccaccgcg tcaccgaaga cgccagccgc gcgaagttct acgcctgctc gatgctgccg
     3061 taccccagcg gcaagctgca catgggccac gtgcgcaact acacgatcaa cgacatgctc
     3121 gcgcgccagc tgcgcatgaa gggcatgaac gtgctgatgc ccatgggctg ggacgccttc
     3181 ggcctgcctg ccgagaacgc ggccatcaag aacaaggtgc cgccggccag gtggacgtac
     3241 gacaacatcg cccacatgaa ggagcagatg aaggcgatgg gcctggccat cgactggtcg
     3301 cgcgaggtgg ccacctgcga cccgacgtac tacaagtgga accagtggct gttcctgaag
     3361 atgctggagg cgggcatctg cgagcgccgc acgcagatcg tcaactggga tccggtggac
     3421 atgaccgtgc tcgccaacga gcaggtgatc gacggccacg gctggcgctc cggcgcgccg
     3481 gtggaaaagc gcgagatccc cggctactac ctcaacatcg tcaagtacgc cgaggagctg
     3541 ctgtctgcgg tggccgatcc ggccgacccg gagtacctgt ccggctggcc cgagcgcgtg
     3601 cgcctgatgc aggagaactg gatcggcaag agcgagggcg tgcgcttcgc gttcccgcac
     3661 acgatccatg acgacgacgg caagctgatc ggcgacggca agctctacgt gttcaccacg
     3721 cgcgccgaca ccatcatggg cgtcacgttc tgcgccgtcg cgcccgagca cccgctcgcc
     3781 acgcacgccg ccaagggcaa cccgcaactg gccgcgttca tcgagacctg caaggccgga
     3841 ggcaccaccg aggccgagct cgccaccaag gaaaaggaag gcctgcccac cgggctgacg
     3901 gtcatccatc cgctcaccgg cgcgcaggtg ccggtgtggg tcggcaacta cgtgctgatg
     3961 acctacggcg acggcgccgt catgggcgtg ccggcgcacg acgagcgcga cttcgcgttc
     4021 gccaggaagt acgggctggc catcaagcag gtggtcgccg tcgccggcga gactttctcc
     4081 accgatgcct ggcaggagtg gtacggcgac aagcagcgcg gcacctgcat cgattcgggc
     4141 gtgctggacg gcctcggcat gaaggacgcg gtgaaccgcg tggccgaact ggtcggcgcc
     4201 gccggcctgg gcgagaagaa gaccacctgg cgcctgcgcg actggggcat cagccgccag
     4261 cgctactggg gcacgcccat ccccatcatc cactgcgacg attgcggcgt ggtgcccgtg
     4321 cccgagaagg atctgccggt cgtgctgccg gaagacctga tccccgacgg ctccggcaac
     4381 ccgctgaaca agcacgccgc cttcctgaac gtgggctgcc ccaagtgcgg caagccggcg
     4441 cgccgcgaga cggacacgat ggacaccttc gtcgattcgt cgtggtactt catgcgctac
     4501 tgcgacccga gcaactcgga gaagatggtc gccgacggca ccgactactg gatgccgatg
     4561 gaccagtaca tcggcggcat cgagcacgcg atcctgcacc tgctttacgc gcgcttctgg
     4621 acgaaggtca tgcgcgacct gaagctcgtc aagttcagcg agccgttcaa gaacctgctg
     4681 acgcagggca tggtgctcaa gggcgcgttc ttccacaagc ccgaggacgc cggcaagaac
     4741 tactactggg agcacgagct cgacgtggaa cgcgacgcgc acggcaacgt cgtgagcgcc
     4801 aagctgaagg acggcacgcc ggtcacctac gagatgacca cgatgtccaa gtccaagaac
     4861 aacggcgtcg acccccaggc cctgatcgac cgctacggcg ccgacacggc gcgcctgttc
     4921 gtgatgttcg cctcgccgcc ggagcagacg ctggagtgga acgactccgg cgtcgagggc
     4981 gcccaccggt tcctcaagcg cgtgtggggc tacggcgtca agaacgctga cgcgctgcgc
     5041 ggcgccgcgg acggcgtcgc cggcctggag ctcgcggcgc ccgccaaggc gctgcgccgc
     5101 gaggtgcacg tgctgctcaa gcaggtcacc tacgactacg agcgcatgca gtacaacacc
     5161 gtcgtctccg gcgcgatgaa gctgctgaac gcgctggaag ggttcgcgct ggacggcagc
     5221 gccggcagcg cggctgtcgc gcgcgaaggc ttctccgtgc ttctgcgctc gctgtatccg
     5281 gcgtgcccgc acatcgcgca ggcgctgtgg acggacatcg gctacggcca gggcaaggaa
     5341 ctcgtggacg ccggctggcc ggccgtcgac gaggccgccc tcgtgcagga cgagatcgag
     5401 ctggtgctgc aggtgctggg caagacgcgc ggcagcgtgc tcgtgcccgc cagcgcggac
     5461 aaggccgcca tcgaggcgct cgcgctggcg tcgccggagg cggtgcgctg gatggaaggc
     5521 aagccggcca ggaaggtcgt gatcgtgccg ggccgcctcg tcaacatcgt cgtctgagcc
     5581 cccgatgcgc gcgcaatcgt ccgtccggcg ccgcgtggcg ctcgccgtcc tcgccgtgtc
     5641 cgcggccgcg acgctggccg cctgcggctt ccatcgccgc atcgcgcagc cgctgaacta
     5701 cgagcggatc gcgctgtcgg gcttcgccga ccgctcgacg atggccgagg agatccggcg
     5761 cgcgctgccg tcgacggcgc gcatcgtctc gaacgtgctg gagtcgcagg tggtgatcga
     5821 ggcggtcgag gacacgcaga agaccaccgt cgaggcgtcc acggccttcg gccaggtgcg
     5881 cgaactcgag ctgcactgca agctgcgcta tcgcgtgctc gacccgaacg gcaccgagct
     5941 gctgccgctg gccgacatcg agcgcttccg cgacctgacc tacgacgaga aggacgccct
     6001 ggccaaggac accgagcaga aggcgctgta ccgcgacatg cagtccgaca tggcctacca
     6061 gctgatccgc gtgctgtcgg ccgtgggcaa gccagccggc ccggccgcgc aggcgcaggc
     6121 ggcgcgcgcg gcgtcggccg cggtggcgcc ggcctcggcg ccgtcgtcgg tctcggcggt
     6181 cgagtgggcc gcgtcgcagg ccgccggccg ctgatcgcca cgccatgcag gtccgccccg
     6241 aacaactgcc cgaccacctg gcgcgcggcc tgcgcgacgt ctacgtggtg cacggcgacg
     6301 agccgctgcg cgcgcaggag gccgccgacg cgatccgcgc ggcggtgcgg gcgggcggcg
     6361 cgagcgagcg caaggtgttc gtggtcagcg gcgcgcactt cgactggagc ggcgtcatcg
     6421 gcgcatcgca ggccatgggc ctgttcgccg accagcagct gatcgagatc cgcatcccct
     6481 cgggcaagcc cggcaaggac ggcagcgagg cgctgcagga gctcgcggcc tcggccggcc
     6541 cggacagccg cctgctggtg cacctgccca agctcgactg gcagcagcag aaggccgcct
     6601 ggttcacgac gctggacgcc gccggcgtca gcgtggcggc cgacatcgtc gagcgcaacg
     6661 cgctgcccgc gtggctcgcc aagcgcttcg ccgcacaggg ccagcgcgtg gaggccggcg
     6721 cggccggcga gcggacgctg gccttcatgg ccgaccgcgt cgagggcaac ctgctcgccg
     6781 cgcaccagga gatccagaag ctgggcctgc tgtacccgcc cggcgagctc acgttcgcgc
     6841 aggtggagga cgccgtgctc gacgtcgccc gccacgacgt ctcgcgcctg tgcgacgccg
     6901 tgatggcggt gcaggtgcca cgcgcgctgc gcatgctcga ggggctgcag gccgagggcg
     6961 aggccgccgt gttcgtgcac tacaagctgt cggacgacat ccgcgccatg aagcgcatcc
     7021 aggacgcgat ggccgaaggc aagccgatgc ccatggcgtt gcgcgaggcc cgcatctggg
     7081 gcgcgcgcga gcggctgttc gagcgcgcgc tgccgcgcct gtccgcgccg atggccgcgc
     7141 gcctggtcag cgccgccagc gagtgcgacg gcgtgctcaa gggcctgcgc catccgcagt
     7201 ggccttccga gccctgggac gcgctgcgcc gcctcgtgct gatgatgctg caggcgctgc
     7261 ggcccgaggg cgcgcaggcc gggctcgtgc tgcgcacctg acgcgcacgg catttgccgc
     7321 aggtccgggc caggaggacg ccatgcttca ccgtcgcctg cttgcactgc tcgtcacgtc
     7381 cgccacgctc gtcgccggct gcgccagccg cgtcgagaag ggggcggccg cgccgatcgc
     7441 gccgctcgac aactccgacc gtgcgagcgc cgcgccgtcc gatcgcgccg aggtcgccgg
     7501 cctgcccgat tggctgcgca tccgcctggc cgactacgac gcgcagtccg gccccgccgc
     7561 gccgcgcgcg gtctacgagg tgcgctacgg cgacggcgtc gcgtactacg tccaggccgg
     7621 ttgctgcgac cagctcgacc cgctggtgga cgcccacggc gtgctcgtgt gctatccgag
     7681 cggcggcttc acgggccgcg gcgacggcaa gtgcctgggc gcgctggcgc cggtggcgga
     7741 gcggcgcgag gtgtggcggc atcgttgagc gacgtcatcg gacggtgcag caggcgtcgc
     7801 ctcgtcgtgt cgccgaatgg cgggttacgc ctgcggccaa cccgccctac gagacttccg
     7861 ccccgatgaa gcggcgtggt gccgcgccac ggcgtcattg gcggcgacgc ggagacaggc
     7921 cccgtggggc gggtcagccg caggcgcaac ccgccatttc cagccgcttg gtcgatcttc
     7981 aacgcgagcc ggcggccttc ccggcttccc gcaggctcat gaacgccttg acgatgtaga
     8041 acaacgccag cgcgtggaag gcgacaggca tccagtcctg ggccttcatg tagatcagcc
     8101 cgtcgtctgg caatggccgg ccgaaagcgg atcgggagtt tcgaggcgtc gtcggccccg
     8161 ggcgggtgcg gcggccgctc agtacccgcg cgtccggtcg acgcgatgcg ccgccgactc
     8221 gccgcgtcgc acggcctcca cgttcgcggc caccaccgcg gcggcactgc gcaggtcggt
     8281 ctgcgcggcg gcgtgcggcc acacggtgat gcgcgggtgg aaccagaaca cgtgcgtggc
     8341 aggcagtggt tcggtgtcga acacgtcgag cacggcgtgc gcgagccggc ccgcgttgag
     8401 cgcctccagc aggtccttct cgacgacgtg cccgccgcgc gccaggttca ccaggctcgc
     8461 gtccgggcgc atctgggcga gccgcgtgcg gtcgaacagg ccgcgcgtgt cctcggtgag
     8521 cggcagcagg ttgacgacga tgtccgccgc gcccagggcc gccggcagcg cggcccagcc
     8581 ggcgctggtg gccatgccgt cgagctgctg cgcgcgggcg ctccagcccg tgacgcggta
     8641 gcccagtgcg gccagttgca gcgccgccgt gcggcccatc tggcccaggc ccagcaccag
     8701 cacggccacc tcgtcggcgc gcttctgcgg cagcgggcgc cactgcgtct ggcgctgctg
     8761 cgcggcgtag tcgaacgcgc gccggtgcag cgacagcacg gcccacgtcg ccgtctccgc
     8821 catcgcccgg ttcatcatcg ggtcgaccat gcgcgcgatc ggcacgtcgc gcggcagcgt
     8881 cgaatcggcg agcagcctgt cgacgccggc ccacagcgac tggatcagcc gcagctgcgt
     8941 gcagttcgcc agccggccag gcagtggatt ggcgacgacg gcgacgtcgg cctcccgcca
     9001 gcgcagcgag ttcgtgccgt cgacgaccgg gtcgatccac tcgtcgtcgg gcagcgcgtc
     9061 ggtgagcgcc cgccaccagt cggcgcgctc ggcgccgtcc atgcgggcgt cgaacgcgat
     9121 cctcatgccc gcgccgccgc gatgaggcgc tccagcgcga tggccaccgt ctgcgcgcgg
     9181 atcgccgccc gatcgccatc gagctgcagc cgttcggtgc ggctgcccac gagcggcgcg
     9241 tcgccaccgg cgggcagccc ttcctgcgtc tcgcgcgggt gcagccatgc cacgcccagc
     9301 cacacggtgc ccaccggctt ggccgcgctg ccgccgccgg gccccgcgat gccggtgatc
     9361 gacagcgcca gctgcacgtc cgcacgcgcg agcgcgccgc gcgccatcgc gtcggccact
     9421 tccgcgctga cggcgccgtg gcgcgcgatc agcggcgcgg gcacgcccag caggccggtc
     9481 ttcgcgtcgt tcgcgtagct gacgaagcct gccgcgaacc agtcgctcgc gccggccggc
     9541 gcggtgcagg cgccggcgag caggccgccg gtgcaacttt ccgcggtggc cagccgccag
     9601 ttgcgggcgc gcagggcgtc gccaagcttt tccactcgcg cgacgaatgc gtcgtcgatc
     9661 gagattccac ggttcgtcgt catctcgaag ctcc
//
LOCUS       DATCRO010000123         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_12521, whole genome shotgun sequence.
ACCESSION   DATCRO010000123 DATCRO010000000
VERSION     DATCRO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12521"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2615)
                     /locus_tag="VIN75_03385"
     CDS             complement(<1..2615)
                     /locus_tag="VIN75_03385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY8973234.1"
                     /translation="MDKHYLQPLLEPEAIVVFTARRGDAQQQTAQGRALTEAIRAQRF
                     TGTIRFLEIGTTGTLAELSQTRADLAVIALPPKDIAAALEVAGRITCKAAVIVSNGVT
                     ADHAAQLRRIAHREGVLLLGPNSLGFQRPKLGLNASAAGPLARTGSLGLVCQSGALTA
                     AMLDWAGANGVGFSLVASIGPHTDVDIAQVLDFLASDPQTQSILVYIEGIGSARRFMS
                     ALRSAAIAKPVVVLKAGRRTAGNEAAQTHSAATVGADDVFDAALRRAGAVRVRSFVEL
                     FSAAKCLASRYRPVGTRLAIVTNGGGPGVLAADWVNEIGLKLGKLSGTTIAALKPMLP
                     DVASISDLIDVSEDATPEHYVAAIRAAAADKDVDGLLAIHSPKAGVDAMAVARAVAGL
                     LPTTYKPLLSCWMGDASMTQARVLLNDAGIASFRTPEAAVGAFGNISTFYQNQRLLQQ
                     TPPPLTTLAQPDIDGARLLIESVLAERRKVLTEMESKTLLSSFHIPVTQTILARSPTE
                     AMMIATQLGFPVALKVDSPDIAHKSDVQGVALNLMSGTSVREAWADMMDRVARLAPNA
                     RINGVTVQKMARARRGREISVGVVTDDPFGPVIVFGAGGVMIELIDDRAMELPPLNQF
                     LARRLIERARVAETLAEWRGAAAADLSALEQVLLRVSEMACALPQLREMDINPIIVDE
                     HGAIAVDARIVVDGGPHAGLGATGQFAHLSILPYPSRFEQLLTLRGGDTCLLRPIRPD
                     DAQMLQTLVRGLSPESRYFRFVSSFNELPATMLSRFTLIDYDREMALVAVVRDRGTGE
                     DGGATETDRIVGVSRYVIAPDQQSCEFSLVVAEDMKGKGLGSRLMESIMEAARERGLA
                     EIAGLVLRSNAPMLKL"
     gene            2795..3085
                     /locus_tag="VIN75_03390"
     CDS             2795..3085
                     /locus_tag="VIN75_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973235.1"
                     /translation="MSDGFGNELPQAHGNTSPRPRSRYLVVIDSGGFMVARLFLESRE
                     QVAEFDASTEEVAQMIGHLPPEDGATDASWDRALAGHSAAERAGARVYVLDL"
BASE COUNT          465 a         1250 c         1252 g          480 t
ORIGIN      
        1 agcttgagca tgggggcgtt gctgcgcagg acgaggccgg cgatctcggc caggccgcgt
       61 tcgcgggccg cctccatgat gctttccatc aggcgcgagc ccaggccctt gcccttcatg
      121 tcctcggcca ccacgagcga gaactcgcag ctctgctggt cgggggcgat gacgtagcgc
      181 gagacgccga cgatgcggtc ggtctcggtg gcgccgccgt cctcgcccgt gccgcggtcg
      241 cgcaccacgg ccaccagcgc catctcgcgg tcgtagtcga tcagcgtgaa gcgcgacagc
      301 atcgtcgccg gcagctcgtt gaacgacgac acgaagcgga agtagcggct ctcgggcgac
      361 aggccgcgca cgagcgtctg cagcatctgc gcgtcgtccg ggcggatcgg gcgcagcagg
      421 caggtgtcgc cgccgcgcag cgtcagcagc tgctcgaagc gcgacgggta cggcaggatc
      481 gacaggtggg cgaactggcc cgtggcgccc aggcccgcgt gcgggccgcc gtcgacgacg
      541 atgcgcgcgt ccaccgcgat ggcgccgtgc tcgtcgacga tgatggggtt gatgtccatc
      601 tcgcgcagct ggggcagcgc gcacgccatc tccgagacgc gcagcagcac ctgctccagc
      661 gcggacaggt cggcggccgc ggcgccgcgc cattcggcca gcgtctccgc cacgcgggcg
      721 cgctcgatca ggcggcgcgc caggaactgg ttcagcggcg gcagctccat cgcgcggtcg
      781 tcgatcagct cgatcatcac gccgcccgcg ccgaacacga tcaccggccc gaacgggtcg
      841 tcggtgacga cgcccaccga gatctcgcgg ccgcggcgcg cgcgcgccat cttctgcacg
      901 gtcacgccgt tgatgcgcgc gttgggcgcc agccgcgcga cgcggtccat catgtcggcc
      961 caggcctcgc gcacgctggt gccgctcatc aggttcagcg cgacgccctg cacgtccgac
     1021 ttgtgcgcga tgtcgggcga gtccaccttc agcgccaccg ggaagccgag ctgcgtggcg
     1081 atcatcatcg cctcggtggg cgaacgcgcg aggatggtct gcgtgacggg gatgtggaac
     1141 gacgacagca gcgtcttcga ttccatctcc gtgagcacct tgcggcgctc ggccagcacg
     1201 ctctcgatca gcagccgcgc gccgtcgatg tcgggctgcg ccagcgtcgt cagcggcggg
     1261 ggcgtctgct gcagcaggcg ctggttctgg tagaacgtgg agatgttgcc gaacgcgccc
     1321 accgcggcct cgggcgtgcg aaagctcgcg atgccggcgt cgttgagcag cacgcgcgcc
     1381 tgcgtcatcg acgcgtcgcc catccagcac gagagcagcg gcttgtacgt ggtcggcagc
     1441 agcccggcca ccgccctggc caccgccatc gcgtcgacgc cggccttggg cgagtggatg
     1501 gccagcaggc cgtcgacgtc cttgtcggcg gccgcggcgc ggatcgcggc gacatagtgc
     1561 tcgggcgtcg cgtcctcgga gacgtcgatc aggtcggaga tcgatgccac gtccggcagc
     1621 atcggcttga gcgccgcgat ggtcgtgccc gacagcttgc ccagcttcag tccgatctcg
     1681 ttgacccagt cggcggccag cacgcccgga ccgccgccat tggtgacgat cgcgaggcgc
     1741 gtgcccacgg gccggtagcg cgaggccagg cacttggccg cggagaacag ctccacgaac
     1801 gaacgcacgc gcactgcgcc ggcgcggcgc agcgcggcgt cgaacacgtc gtcggcgccc
     1861 accgtcgcgg cgctgtgcgt ctgcgcggcc tcgttgccgg ccgtcctgcg cccggccttg
     1921 agcacgacca ccggcttggc gatggccgcc gagcgcagcg cgctcatgaa gcggcgcgcg
     1981 ctgcctatgc cctcgatgta gaccaggatg ctctgcgtct gcgggtcgct tgcgaggaag
     2041 tccagcacct gcgcgatgtc gacgtcggtg tgcgggccga tggacgccac cagcgagaag
     2101 ccgacgccgt tggcgccggc ccagtccagc atggcggcag tcagcgcgcc cgactggcac
     2161 accaggccca gcgacccggt gcgtgccagc ggcccggcgg cgctcgcgtt gaggcccagc
     2221 ttcgggcgct ggaagcccag gctgttcggg cccagcagca gcacgccctc gcggtgcgcg
     2281 atcctgcgca actgcgcggc gtggtcggcc gtgacgccgt tggagacgat gaccgccgcc
     2341 ttgcaggtga tgcggccggc cacctcgagc gcggcggcga tgtccttggg cggaagcgcg
     2401 atgacggcca ggtcggcgcg cgtctgcgac agctcggcca gcgtgcccgt cgtgccgatc
     2461 tccaggaagc ggatcgtgcc ggtgaagcgc tgggcgcgga tcgcctcggt gagcgcgcgg
     2521 ccctgcgccg tctgctgctg cgcgtccccg cggcgggccg tgaacacgac gatggcctcg
     2581 ggctcgagca gcggctgcag gtagtgcttg tccatgcccc tagtctgcgt caaaaaggcg
     2641 cggcaagggc ggcgggcacg gcatggccga ctccggccgg gacttgacgg cccatcgccg
     2701 gatccgcgcc cccggatgcg gcgcgcgcga ccggacgtcc aggcgtacga tcggccaacc
     2761 ctgccgtgca tcgcccggcg ggcgggagat cccaatgagc gacggattcg gcaacgagct
     2821 tccccaagcg cacggcaaca cctcgccccg cccgcgctcg cgctacctgg tcgtcatcga
     2881 ctcgggcggc ttcatggtgg cgcgcctgtt cctcgagtcg cgcgagcagg tcgccgagtt
     2941 cgacgcgagc acggaggagg tcgcgcagat gatcgggcat cttccgcccg aggacggcgc
     3001 gaccgacgcg tcctgggacc gcgccctggc cggccacagc gcggccgagc gtgccggggc
     3061 acgggtgtac gtgctcgatc tctgaacccg tcccgcgtcg cctgacgtcc cgcccgcagc
     3121 cacgcgccac gctttcgagg gatggcacga ggcgggcccg gatcccacac tgggcgcttg
     3181 cagatcggcg ccgggttccc gttgcaacgg agggcatgaa ccatgatgtc gacaggcccg
     3241 gccgctgccg cgccgatcca ccgcctgtcg cggcgcgtcc cctccgcagg agcggccatg
     3301 gagcccccct cgacgccgac gacgcccgcc gcccgcaccc aggcccgcct gaggcgcccg
     3361 gccgggtcgt cccgggtcct gcgggtggcc tggcagctcg tgctgggcac ggtggggttc
     3421 gccggcttcg cgctgctggg cgccggc
//
LOCUS       DATCRO010000124         5435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1115057, whole genome shotgun sequence.
ACCESSION   DATCRO010000124 DATCRO010000000
VERSION     DATCRO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115057"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="argG"
                     /locus_tag="VIN75_03395"
     CDS             complement(<1..912)
                     /gene="argG"
                     /locus_tag="VIN75_03395"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY8973236.1"
                     /translation="MTTILQHLPAQQKVGIAFSGGLDTSAALHWMRLKGAIPYAYTAN
                     LGQPDEPDYDEIPRKAMQYGAEKARLIDCRAQLVAEGIAALQSGAFHISTAGVTYFNT
                     TPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGMLANPSLKIYKPWLDQ
                     VFIDELGGRTEMSEFLNKAGFGYKMSVEKAYSTDSNMLGATHEAKDLEHLNSGIKIVQ
                     PIMGVAFWRDDVVVKAEEVRVRFHEGMPVALNGKEFADHVELMMEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFAAY"
     gene            1068..2246
                     /locus_tag="VIN75_03400"
     CDS             1068..2246
                     /locus_tag="VIN75_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY8973237.1"
                     /translation="MTAPTQPPPRTPIVLAFALALAAAVSLGLSRFSYALLLPPMRAQ
                     LHWSYFTAGAMNTANAAGYLAGALLARRWFARHDALRVATAGGAATAAILVAHAFATS
                     DAPLYALRTLAGVASAATFVGGGLLATRLSARAPHPGLVVGLYYGGAGLGILGSTALV
                     PPLPWREAWVALGVVSLACALATWGVTRASRALVATGPDAPSAGRTRAAWAPMAPLLA
                     GYLMFGFGYIGYMTFIVTLLRDQGLSPGRVAAFFGLLGACTVASSWLWAGLLQRHRGG
                     RPLAQLNAILALATLLPVLGTQPVMVGLSCVLFGSCFLSVVASTTAFVRHNLAPPAWA
                     AGIGAFTIVFALGQILGPSLSGLLADGPGGLRRGFVFSAIVLALGALLGARQRPLAPR
                     "
     gene            complement(2298..3386)
                     /locus_tag="VIN75_03405"
     CDS             complement(2298..3386)
                     /locus_tag="VIN75_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HEY8973238.1"
                     /translation="MTTRLLKTSLAAAALACCGAAMADVTFYEYDNYGGRSFTVHDPI
                     ESFQRIGFNDRASSVVVTGRPWEVCDDSGFHGHCYILRPGNYPSLSSMGLGERISSAR
                     WVDRNGNFPPDRWAPPPLPGQVTLYERDNFRGPSFSTTSDIDNLRDVGFNDRASSLVV
                     VGDRWEACDDRGYSGHCVFLRPGNYPNLDAMGMGNSISSVRLTPADQPVADAGWAPVP
                     PPAYDARRRPQEQLFQAQVLATRAVYGTPTQRCWVEQAEVRDDRNRVGGTVIGGILGG
                     ILGHQVARGHGAVAAGAIGGALVGSAIGGANSGTRLEDVQRCTTDESGPPQFWDTVYT
                     FNGVEHHVQTTAPAGATITVNQYGEPRI"
     gene            complement(3474..4808)
                     /locus_tag="VIN75_03410"
     CDS             complement(3474..4808)
                     /locus_tag="VIN75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973239.1"
                     /translation="MDRKHLSLSLFWRTFFLLSLLLTGGVIAWVQTLRQLEFEPRAVV
                     AAQQIASLVNLSRGAMKNVDGITRIALMKSMTDQEAVRVLPREPTDRWDPYEVDAFTR
                     RIAAELRVRLGEGTVVARSVNGQDALWVGFTIDDDRYWLQAESARVTPLTSSTWFVWV
                     GIAILATVIGSATIAGLINRPLKDLSTATARIREGEYDIRLDERVGTPEVRAVNIGFN
                     RMARDLAKVEQDRTVMLAGISHDLRTPLARIRLEAEISVPDDEARAHIAEDIDQLDAI
                     IDKFMDYARPGVTRLEPVPLAAVVEREALAFRDASQIRIAIQLSPVSRVLGDPIELGR
                     VFANLFENARRYGRPSDTVPAEVQVGQLQAGPWIIVTVRDRGPGVPVEKLAQLTTPFF
                     RGDTARTSATGAGLGLAVVKKSIQRMGGSLEMTNAPEGGLVAHVRLRRVEAT"
     gene            complement(4832..>5435)
                     /gene="ompR"
                     /locus_tag="VIN75_03415"
     CDS             complement(4832..>5435)
                     /gene="ompR"
                     /locus_tag="VIN75_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY8973240.1"
                     /translation="LNRLMTRENIDLIVLDLMLPGEDGLSICRRLRAAGDRTPIIMLT
                     AKVEDVDRIVGLEVGADDYLPKPFNPRELLARVHAVLRRRPPQEAPGAPAKDAQVVQF
                     GDFEFDLSLRRLTKDGEPIALTTGEFSMLKALVRHPRQPLSRDKLAQLARGRDFEPFD
                     RSLDVQISRLRKMIEPDPSNPRYIQTVWGVGYVFVPSETA"
BASE COUNT          698 a         1865 c         1993 g          879 t
ORIGIN      
        1 gtaggcggcg aacagcagcg ccaggcccgg ggcctcgtag atgccgcggc tcttggcctc
       61 gatgatgcgg ttctcgatct ggtcgctcat gcccaggccg tggcggccgc cgatgcggtt
      121 ggcctccatc atcagctcga catggtcggc gaattccttg ccgttcaagg ccacgggcat
      181 gccctcgtgg aagcgcacgc gcacctcctc ggccttgacg acgacgtcgt cgcgccagaa
      241 cgccacgccc atgatgggct gcacgatctt gatgccggag ttcaggtgct cgaggtcctt
      301 ggcctcgtgc gtggcaccca gcatgttgga gtcggtcgaa taggccttct cgaccgacat
      361 cttgtagccg aatcccgcct tgttcaggaa ctccgacatc tcggtgcggc cgccgagctc
      421 gtcgatgaac acctggtcca gccaaggctt gtagatcttg agcgacgggt tcgcgagcat
      481 gccgtagcgg tagaagcgct cgatgtcgtt gcccttgaag gtggagccgt cgccccagat
      541 gttgacgtcg tcctccttca tcgcggccac cagcatcgtg ccggtcaccg cgcggcccag
      601 gggcgtggtg ttgaagtagg tgacgccggc ggtggagatg tggaacgcgc cgctctgcag
      661 cgcggcgatg ccctcggcca ccagctgcgc gcggcagtcg atcaggcgcg ccttctcggc
      721 gccgtactgc atggccttgc gcgggatctc gtcgtagtcc ggctcgtcgg gctgtcccag
      781 gttcgcggtg taggcgtagg ggatcgcgcc cttcaggcgc atccagtgca gcgcggcgct
      841 ggtgtcgagg ccgccggaga aggcgatgcc gaccttttgc tgggcgggca ggtgctggag
      901 gatggtggtc atgtcggttc cggttccggg aagggcgaac tgcgggtaag ccctctattc
      961 tcgactgaaa tcggcccgat ttcccacgcc cggttgtcta gacgagagga tgcgagccgg
     1021 atttccccgg ccgggcgcgt gcgtgcgtcc gcggggatac tcgatccgtg accgccccca
     1081 cgcagccgcc tccccgcacg ccgatcgtcc tcgccttcgc gctggcgctc gcggccgccg
     1141 tgtcgctggg gctgtcgcgc ttctcgtacg cgctgctgct gccgccgatg cgcgcgcagc
     1201 tgcactggag ctatttcacc gccggcgcga tgaacaccgc caacgccgcg ggctacctgg
     1261 ccggcgcgct gctcgcacgg cgctggttcg cgcgtcacga cgccctgcgc gtggccacgg
     1321 ccggcggggc ggccacggcg gccatcctcg tcgcgcacgc cttcgccacg agcgatgcgc
     1381 cgctgtatgc attgcgaacg ctggcgggcg tggccagcgc cgccaccttc gtcggcggcg
     1441 gcctgctcgc gacacggctg tcggcgcgcg cgccgcatcc ggggctcgtg gtcggcctgt
     1501 actacggcgg cgcggggctg ggcatcctgg gttcgacggc cctcgtgccg ccgctgccgt
     1561 ggcgcgaggc gtgggtggcg ctgggcgtcg tgtcgctcgc gtgcgcgctc gcgacctggg
     1621 gcgtgacgcg cgcgtcgcgc gcgctcgtgg ccaccggccc ggacgcgccg tcggccggac
     1681 gcacgcgcgc cgcgtgggcg ccgatggcgc cgctgctggc cggctacctg atgttcggct
     1741 tcggctacat cggctacatg accttcatcg tcacgctact gcgcgaccag ggactgtcgc
     1801 ccggccgcgt ggccgcgttc ttcgggctgc tgggcgcctg cacggtggcg tcgtcgtggc
     1861 tctgggccgg cctgctgcag cgccatcgcg gcgggcggcc gctggcgcag ctcaacgcga
     1921 tcctggccct cgccacgctg ctgcccgtgc tcggcacgca gcccgtgatg gtgggactgt
     1981 cgtgcgtgct gttcggctcg tgcttcctgt cggtggtggc gtcgaccacc gcgttcgtgc
     2041 gccacaacct ggcgccgccg gcgtgggccg cgggcatcgg cgcgttcacg atcgtgttcg
     2101 cgctgggcca gatcctcggg ccgagcctgt ccggcctgct ggccgacggg cccggcgggc
     2161 tgcgccgcgg cttcgtgttc tcggcgatcg tgctggcgct gggcgccttg ctcggcgcgc
     2221 gccagcggcc gctcgcgccg cgttgaacga agggacggcg gccgcagccg ccgtcgatgc
     2281 acggacgccg cgcggcgtca gatgcgcggt tcgccgtact ggttcacggt gatcgtggcg
     2341 ccggccgggg cggtcgtctg cacgtggtgt tccacgccgt tgaacgtgta gaccgtgtcc
     2401 cagaactgcg gcgggccgga ttcgtcggtg gtgcagcgct gcacgtcctc gaggcgcgtg
     2461 ccgctgttgg cgccgccgat cgcgctgccc accagcgcgc cgccgatggc gccggccgcg
     2521 accgcgccat ggccacgcgc cacctggtgg cccaggatgc cgccgaggat gccgccgatc
     2581 acggtgccgc cgacgcggtt gcgatcgtcg cgcacctcgg cctgctcgac ccagcagcgc
     2641 tgcgtcggcg tgccgtagac cgcgcgcgtc gcgagcacct gcgcctggaa cagctgctcc
     2701 tgagggcggc ggcgcgcgtc gtacgcgggc ggcgggacgg gcgcccagcc ggcgtcggcc
     2761 acaggctggt cggccggcgt caagcggacc gaactgatgc tgttgcccat gcccatcgca
     2821 tcgaggttcg gatagttgcc cggacgcagg aagacgcagt ggccgctgta gccgcggtcg
     2881 tcgcaggcct cccagcggtc gccgaccacc accagcgacg acgcgcggtc gttgaagccg
     2941 acgtcgcgca ggttgtcgat gtcggaagtc gtgctgaacg acggcccgcg gaagttgtcg
     3001 cgctcgtaga gcgtcacctg gcccggcagc ggcggcgggg cccagcggtc gggtgggaag
     3061 ttgccgttgc ggtcgaccca gcgcgccgac gagatgcgtt cgcccaggcc catcgagctc
     3121 agcgacgggt agttgccggg ccgcaggatg tagcagtggc cgtggaagcc cgagtcgtcg
     3181 cagacttccc acggacggcc ggtcaccacc accgacgagg cgcggtcgtt gaagccgatg
     3241 cgctggaagc tctcgatcgg gtcgtggacg gtgaaggatc gcccgccgta gttgtcgtat
     3301 tcgtagaaag tgacatcggc catcgccgcg ccgcagcagg cgagggcggc cgccgcgagc
     3361 gaggtcttga gcagtctggt tgtcatcgga cactctccgt tgtcgtgatg caacggaacg
     3421 ggcaatcgag gggcccgtct ttgcgcgacc gcaacgccgc gcgggtccgg aaatcaggtc
     3481 gcttcgaccc tgcggagacg cacgtgcgcg accaggccgc cctcgggcgc gttggtcatc
     3541 tccaggctgc cgcccatgcg ctggatcgac ttcttgacca ccgccaggcc caggcccgcg
     3601 ccggtggccg acgtgcgtgc cgtgtcgccg cggaagaagg gcgtggtcag ctgcgcgagc
     3661 ttctccaccg gcacgccggg gccgcgatcg cgcaccgtca cgatgatcca cggtccggcc
     3721 tgcagctggc ccacctgcac ctcggcgggc acggtgtcgg acgggcggcc gtagcggcgc
     3781 gcgttctcga acaggttggc gaacacgcgc cccagctcga tcggatcgcc cagcacgcgc
     3841 gagaccggcg acagctggat ggcgatgcgg atctggctcg cgtcgcggaa ggccagtgcc
     3901 tctcgttcga cgacagcggc cagcggcacc ggctccaggc gcgtgacgcc cggacgcgcg
     3961 tagtccatga acttgtcgat gatcgcgtcg agctggtcga tgtcctcggc gatatgggcg
     4021 cgggcctcgt cgtcgggcac gctgatctcg gcctccaggc ggatgcgcgc cagcggcgtg
     4081 cgcaggtcgt gcgagatgcc ggccagcatc acggtgcggt cctgttcgac cttcgcgagg
     4141 tcgcgcgcca tgcggttgaa gccgatgttg acggcacgca cctcgggcgt gccgacgcgt
     4201 tcgtcgagcc ggatgtcgta ctcgccctcg cggatgcggg ccgtggccgt cgacagatcc
     4261 ttcagcggcc ggttgatcag gccggcgatc gtcgccgagc cgatgacggt ggccaggatg
     4321 gcgatgccca cccacacgaa ccaggtgctg gacgtcagcg gcgtgacacg cgccgattcg
     4381 gcctgcagcc agtagcggtc gtcatcgatc gtgaagccca cccacagcgc gtcctgcccg
     4441 ttgaccgagc gcgcgacgac ggtgccttcg cccaggcgca cgcgcagctc ggccgcgatg
     4501 cggcgcgtga aggcgtcgac ctcgtacgga tcccagcggt cggtcggctc gcgcgggagc
     4561 acgcgcacgg cctcctggtc ggtcatcgac ttcatcagcg cgatgcgcgt gatgccgtcg
     4621 acgttcttca tcgcgccgcg cgacaggttc acgaggctgg cgatctgctg ggcggccacg
     4681 accgcgcgcg gctcgaattc gagctggcgc agcgtctgga cccaggcgat gacgccgccg
     4741 gtcagcagca gcgacagcag gaagaacgtg cgccagaaga gactcagcga caggtgcttg
     4801 cggtccatcc ccggcttttt gctgaaggtg gtcaggccgt ctcgctgggg acgaacacgt
     4861 agcccacgcc ccacacggtc tggatgtagc gcgggttcga cggatcgggc tcgatcatct
     4921 tgcgcaggcg cgagatctgc acgtccaggc tgcggtcgaa gggctcgaag tcgcggccgc
     4981 gcgcgagctg ggccagcttg tcgcgcgaca gcggctggcg cgggtggcgc acgagcgcct
     5041 tgagcatcga gaactcgccg gtggtcagcg cgatcggctc gccgtccttg gtcaggcggc
     5101 gcagcgacag gtcgaactcg aagtcgccga actgcaccac ctgggcgtcc ttggcggggg
     5161 cgccgggcgc ctcctgcggc gggcggcggc gcagcaccgc gtgcacgcgg gccagcagct
     5221 cgcgcgggtt gaagggcttg ggcaggtagt cgtcggcgcc cacctcgagg ccgacgatgc
     5281 ggtccacgtc ctcgaccttg gcggtgagca tgatgatggg cgtgcggtcg ccggcggcgc
     5341 gcaggcggcg gcagatcgac aggccgtcct cgcccggcag catcaggtcg agcacgatca
     5401 ggtcgatgtt ctcgcgcgtc atcaggcggt tgagc
//
LOCUS       DATCRO010000125         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_473998, whole genome shotgun sequence.
ACCESSION   DATCRO010000125 DATCRO010000000
VERSION     DATCRO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473998"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIN75_03420"
     CDS             <1..317
                     /locus_tag="VIN75_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8973241.1"
                     /translation="TAPPAILFVTLDPLNDTSKRLRDYLSVFDPRVIGLTGEPDQVRR
                     IAQHYGVGIARQGSSLAHSSRWYVVDADGRIDRVYAYDTPPDRLAAALHLPQSIAQET
                     SR"
     gene            314..1906
                     /locus_tag="VIN75_03425"
     CDS             314..1906
                     /locus_tag="VIN75_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8973242.1"
                     /translation="MTRLRSLLARIRVPRPTGIAGRLGLGFAALLAVMTIVVGVAAWQ
                     ARTIANQLDAVVNEQIPRMLKVGALARVVADLNLAARDAIISAGEPAGDAALARIESG
                     RSQVGQQIEDLQRALRTGDPRGEKAATELGDQSSGILVALVKFGRVLKAHNMDMARGL
                     LSSNVQPRIGALGSAVDNAQALQQQMLGEASERSNRAWRIALAEIAATLVGAVALATL
                     LAWRIARGVTNPVNDAVRLAERIAAGDLRETLAVDRSDELGRLQRALADMQARLGLLV
                     GQIHDVAGHMAQASAEVSSGSDDLSHRTEQAASALQEAASSMTLLSGQVRESAQAASS
                     ANELAANTAAVAQRGGELVGKVIESMKDIARASHRIGEITGVIDGISFQTNILALNAA
                     VEAARAGEHGRGFAVVAGEVRSLAQRAATAAKEIRALITASTEQVDSGSRLVQTAGAT
                     MSDIVDGARKVTGIVSGISDASERQSGDLGRVNETVGGLDAMTQQNSALVEQSAAAAQ
                     NMREQSLRLQAMVNTFKLAPSA"
     gene            complement(1986..2897)
                     /locus_tag="VIN75_03430"
     CDS             complement(1986..2897)
                     /locus_tag="VIN75_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEY8973243.1"
                     /translation="MAFASGFSRDDNPNFLRNAVIGVVVLIVLVAFWPIRTVPTGSRG
                     VITVGGAIRGLQNEGFTVILPWQKLDVFSIRAEQADIENAEGSTSDTQPVKVSLTVRY
                     SIAPDRVTEVYEKYSHDGNLQSYVQTATQEVFKAVTARYSAPDLIAQRAAVSGDITGA
                     LRSKLATYGAQVISIDMRNFSFSESYMRAINDKVTQEQLRLAAENKLKTVEAEQKQKV
                     AVAEAEANAVKATADGDAYAKLKVAQAEAQAMQVQNEALAKNKDVLELRRIEVELTKA
                     QKWNGQLPQAIYAGAPVPFMNVGNPAK"
     gene            complement(2957..>3549)
                     /locus_tag="VIN75_03435"
     CDS             complement(2957..>3549)
                     /locus_tag="VIN75_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEY8973244.1"
                     /translation="TAAIDAAAKRMEAARAQMEAAQKSGDAASSAAGLGAMMGALTGA
                     GGAAIPPADLKAQLPDTLGALKRESFETSGGTAVGIASSTAKATYAGGDNQRAELSIT
                     DVGGLGGLASVATWANVTVDKETPDGIEKTYKDGARTIHEQYRKDGSHSEYTVILKNG
                     VIVETQGDHIDGATLKSMASAVNLDALEAMKRPAKT"
BASE COUNT          523 a         1223 c         1227 g          576 t
ORIGIN      
        1 gcacagcacc gccagcgatc ctcttcgtga cgctcgaccc cttgaacgac acgtcgaagc
       61 gattgcgcga ctacctgagc gtgttcgacc cgcgcgtgat cggcctgacc ggcgagcccg
      121 atcaggtccg gcgcatcgcg cagcactacg gcgtcggtat cgcacgccaa ggatcgtcgc
      181 tggcccacag ctcccgctgg tacgtcgtcg acgccgatgg ccggatcgat cgggtctacg
      241 cctacgacac gccaccagat cgcctggccg cagccttgca tcttcctcag tccatcgccc
      301 aggagacctc gcgatgacac ggctccgttc cctcctggcc cgaatccgcg tgcccaggcc
      361 gaccggcatc gccggccgcc tcggcctggg gttcgctgcc ctgctcgcgg tgatgacgat
      421 cgtcgtgggc gtagcggcct ggcaggcgcg cacgatcgcg aaccagctcg atgcagtggt
      481 caacgagcag atcccgcgca tgctcaaggt cggtgcgctg gcacgcgtcg tcgcggacct
      541 gaacctcgcc gcccgcgacg cgatcatctc cgccggcgag ccggccggtg atgcggcgct
      601 tgcgcgcatc gaatccggcc gctcgcaggt cgggcagcag atcgaggacc tgcagcgcgc
      661 cttgcgcacg ggcgatccgc ggggagagaa ggccgccacc gaactcggcg atcagagcag
      721 tggcattctg gtggcgctgg tcaagttcgg tcgagtattg aaggcccaca acatggacat
      781 ggcgcgcggc ctgctctcga gcaacgtcca gccgcgcatc ggtgcgctgg gctcggccgt
      841 cgacaacgcg caggcgctgc agcagcagat gctcggagag gcaagcgaac gcagcaatcg
      901 cgcatggcgc atcgctctgg cggagatcgc ggccacgctg gtcggcgccg tcgccctggc
      961 cactctgctc gcgtggcgga tcgcgcgggg cgtgacgaac cccgtgaatg acgccgtccg
     1021 gctggccgaa cgcatcgcgg cgggcgatct gcgcgagacc ctggccgtgg accgcagcga
     1081 cgagctcggc cgcctgcagc gggccctcgc ggacatgcag gcgcggctcg gtcttctcgt
     1141 cggccagatc cacgacgtcg ccggacacat ggcgcaggcc agcgccgaag tctccagcgg
     1201 cagcgacgac ctgagccacc gcacggaaca ggccgccagc gcgctccagg aggccgcaag
     1261 ttcgatgacg ctgctcagcg gccaggtgcg cgagagcgcg caggccgcca gttccgccaa
     1321 cgagcttgcc gccaacactg ccgccgtggc ccagcgtggc ggcgagcttg tcggcaaggt
     1381 catcgagagc atgaaggaca tcgcgcgggc gagccatcgc atcggagaga tcaccggcgt
     1441 catcgacggc atctctttcc agacgaacat cctggcgctg aacgccgccg tcgaggccgc
     1501 tcgcgcgggc gagcacggtc gcgggttcgc agtggtcgcg ggcgaagtcc gcagcctcgc
     1561 gcagcgcgcc gccaccgcgg ccaaggagat ccgggcgctc atcacggcca gcaccgagca
     1621 ggtcgattcg ggaagccgtc tcgttcaaac ggcgggcgcg acgatgagcg acatcgtcga
     1681 cggcgcacgc aaggtcacgg gcatcgtctc tggcatcagc gacgcctcgg agcgccaaag
     1741 cggcgacctt gggcgcgtca acgaaaccgt tggcggactg gacgcaatga ctcagcaaaa
     1801 ctccgcgctg gtcgagcaga gtgccgccgc cgcgcagaac atgcgcgagc agtcgctgcg
     1861 gctgcaagcg atggtcaaca cgttcaagct cgctccctcg gcctgacggc gctttggcgt
     1921 gcgtcgtccg aatacgcgct gccgagcggc gccgaacgac cgcggcctcg cgagcgtgcc
     1981 agatctcact tcgccgggtt gcccacgttc atgaacggca ccggcgcgcc cgcgtagatg
     2041 gcctgcggca gctggccgtt ccatttctgc gccttggtga gctcgacctc gatgcggcgc
     2101 agctccagca cgtccttgtt cttggcgagc gcctcgttct gcacctgcat cgcctgcgcc
     2161 tcggcctggg cgaccttcag cttggcgtac gcgtcgccgt cggcggtggc cttgacggca
     2221 ttggcctcgg cctcggccac ggcgaccttc tgcttctgct cggcctcgac cgtcttgagc
     2281 ttgttctcgg cggccagccg cagctgttcc tgcgtgacct tgtcgttgat cgcgcgcatg
     2341 tagctctcgc tgaacgagaa gttgcgcatg tcgatggaga tcacctgcgc gccataggtg
     2401 gcgagcttgg agcgcagcgc gcccgtgatg tctccggaga cggcggcgcg ctgggcgatc
     2461 aggtcgggtg cggagtagcg cgccgtcacc gccttgaaca cctcctgcgt ggcggtctgc
     2521 acgtagctct ggaggttgcc gtcgtggctg tacttctcgt agacctcggt gacgcggtcg
     2581 ggcgcgatcg agtagcgcac cgtcaggctc accttgaccg gctgggtgtc ggacgtggag
     2641 ccttccgcgt tctcgatgtc ggcctgctcg gcgcggatgc tgaacacgtc cagcttctgc
     2701 cacggcagga tgacggtgaa gccctcgttc tgcagcccgc ggatggcgcc acccacggtg
     2761 atcacgccgc gcgagccggt gggcacggtg cggatcggcc agaacgccac caggacgatc
     2821 agcacgacga cgccgatcac ggcattgcgc aggaaattcg ggttgtcgtc cctggagaag
     2881 ccggaagcga aggccatgga tgtctgcggc agttgagatg gtcgcgactc tacgccagct
     2941 cgaccgcagc gtcagctcag gtcttggcgg ggcgcttcat cgcctcgagt gcatcgaggt
     3001 tgaccgccga ggccatggac ttgagcgtgg cgccgtcgat gtggtcgccc tgcgtctcga
     3061 cgatcacgcc gttcttcagg atgacggtgt attcggaatg gctgccgtcc ttgcggtact
     3121 gctcgtggat cgtgcgcgcg ccgtccttgt acgtcttctc gatgccgtcg ggcgtctcct
     3181 tgtcgacggt gacgttggcc caggtggcca ccgaggcgag gccgccgagg ccgccgacat
     3241 cggtgatcga cagctcggcg cgctggttgt cgccgcccgc gtaggtggcc ttggccgtgg
     3301 agctggcgat gcccaccgcg gtgccgccgc tggtctcgaa ggactcgcgc ttgagggcgc
     3361 cgagcgtgtc gggcaactgc gccttcaggt cggcgggcgg gatggccgcg ccgccggcgc
     3421 cggtcagcgc gcccatcatc gcgcccagcc cggcggcgct ggaggccgcg tcgcccgact
     3481 tctgcgccgc ctccatctgc gcgcgggccg cttccatgcg cttggcggcc gcatcgatgg
     3541 cggcggtgt
//
LOCUS       DATCRO010000126         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_991361, whole genome shotgun sequence.
ACCESSION   DATCRO010000126 DATCRO010000000
VERSION     DATCRO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991361"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIN75_03440"
     CDS             <1..286
                     /locus_tag="VIN75_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8973245.1"
                     /translation="TGGIAKRTPADRVAAMRDRIDAFDRTLRSVGKVHKGDVVDLDWL
                     PSEGLAMRVNGKAVGTPVPGADLYTAMLDIFVGDRPVDAKMKAGMLGAAS"
     gene            342..1235
                     /gene="xerD"
                     /locus_tag="VIN75_03445"
     CDS             342..1235
                     /gene="xerD"
                     /locus_tag="VIN75_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8973246.1"
                     /translation="MLRSAGAIDRFLDGLWLEDGLARNSLAAYRRDLTLFAAWLGQEQ
                     ARDLAQARASDVQGYMLARHAASKATSANRRLAVFRRFYRWGLRERLVDEDPTLKLRP
                     ARQPMRVPKAITEAQVDALLAAPDTGTPLGARDRAMLELMYASGLRVSELVDCKLVDL
                     SLPDGVVRVMGKGSKERLVPFGDEARRWLEAWLATSRPELLEGRRSDALFVTVRAAAM
                     SRQMFWNLVKKYALAAGIRNAISPHTLRHAFATHLLNHGADLRAVQLLLGHADISTTT
                     IYTHVARERLRQLHSKHHPRG"
     gene            complement(1258..1749)
                     /locus_tag="VIN75_03450"
     CDS             complement(1258..1749)
                     /locus_tag="VIN75_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973247.1"
                     /translation="MHVRSFAFKACALLLASLASPLAAAAPQPLNETEMSSVRGADGS
                     ILVGLQPSSSNSQNPFASGLSAAFASSTGPTTLTPAQFATALQAVGAAPSNFADYAGQ
                     PVAQSVVDAKPVTFSFNFSDVLQSTTGLSVGTGGTGSPSLGTFTLKDFDARGTVIWVW
                     QHH"
     gene            complement(1920..2519)
                     /locus_tag="VIN75_03455"
     CDS             complement(1920..2519)
                     /locus_tag="VIN75_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8973248.1"
                     /translation="MPKPSHREKLLQVGMQVVHERGFRGASVRDIVQAAGVPQGSFTN
                     HFASKEAFGLEVVERYAAQADTAMRQTLRNEHLPPLQRLRAFIERSKTGFAEDGLRNG
                     CLLGNFSAEANDCGEPLRGRVVAALADMQDALAFCLRAAVSGGELAPSTDCDGTAEFV
                     LAALQGATLLAKAQRSPRPLERFEDVLFERVLERATPVR"
     gene            2702..>3298
                     /locus_tag="VIN75_03460"
     CDS             2702..>3298
                     /locus_tag="VIN75_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY8973249.1"
                     /translation="MDHTFLSAFILLLLVLDPFGSLPIFISILGSVAPERRTFVAVRE
                     VLIAFGVLVLFMVSGQGFLTLMRLSERSLEVAGGVILAIIAIRMIFGGGAEIYGADTA
                     KREPFIFPLAVPLLAGPSAMATVLLLASRQPEHLVTWIGALTAALAISGVVLLAADRI
                     RRVLGTQMVAAIEKLMGLVLTAVAVEMILAGLKRYFIGD"
BASE COUNT          448 a         1162 c         1197 g          491 t
ORIGIN      
        1 caccggcggc atcgccaagc gcacgcccgc cgaccgggtg gccgcgatgc gcgatcgcat
       61 cgatgcgttc gaccgcacgc tgcgcagcgt cggcaaggtg cacaagggcg acgtggtcga
      121 cctcgactgg ctgccctccg aaggcctggc catgcgcgtc aacggcaagg cggtgggcac
      181 gccggtgccg ggcgccgatc tctacaccgc gatgctggac atcttcgtcg gcgaccgccc
      241 ggtcgacgcg aagatgaagg ccggcatgct gggcgcggcg agctgacttg gccccgcgca
      301 agaagaccgc cgcccctggc gccccgcccc ccggcgcggc gatgcttcgc agcgccggcg
      361 cgatcgaccg cttcctcgac ggcctgtggc tggaggacgg cctcgccagg aactcgctcg
      421 cggcctaccg gcgcgacctg accctgttcg cggcctggct gggccaggag caggcgcgcg
      481 acctcgcgca ggcgcgcgcg agcgacgtgc agggctacat gctcgctcgc cacgccgcga
      541 gcaaggccac cagcgcgaac cggcggctgg cggtgttccg gcgcttctac cgctgggggt
      601 tgcgcgagcg cctcgtcgac gaggacccga cgctcaagct gcgcccggcg cgccagccga
      661 tgcgcgtgcc caaggccatc accgaggcgc aggtcgatgc gctgctcgcg gccccggaca
      721 cgggcacccc gctgggcgcc cgcgaccgcg cgatgctgga gctgatgtac gccagcggcc
      781 tgcgcgtgag cgagctcgtc gattgcaagc tggtcgatct cagcctgccc gacggcgtcg
      841 tgcgcgtgat gggcaagggc tccaaggagc gcctcgtgcc gttcggcgac gaggcgcggc
      901 gctggctcga ggcgtggctc gccacgtcgc ggcccgagct gctcgagggc cggcgcagcg
      961 acgcgctgtt cgtgacggtg cgcgcggcgg cgatgtcgcg ccagatgttc tggaacctgg
     1021 tcaagaagta tgcgctggcc gcgggcatcc gcaacgcgat ctcgccgcac acgctgcgcc
     1081 acgcgttcgc cacgcacctg ctcaaccacg gtgccgacct gcgcgccgtg cagctgttgc
     1141 tggggcacgc cgacatctcg acgacgacga tctacaccca tgtggcgcgc gagcgcctgc
     1201 gccagctgca ttcgaagcat catccacggg gatgacccgc ggatcggcgg cagcgtctca
     1261 gtggtgctgc cagacccaga tgaccgtgcc gcgggcgtcg aagtccttca gcgtgaacgt
     1321 gcccagcgac gggctgccgg tgccgccggt gccgaccgac aggccggtgg tcgactgcag
     1381 gacgtccgag aagttgaagc tgaaggtcac gggcttggcg tcgacgaccg actgcgcgac
     1441 cggctggccc gcgtagtcgg cgaagttcga cggcgccgcg cccacggcct gcagcgcggt
     1501 ggcgaactgt gccggggtca gcgtggtggg gccggtgctg ctcgcgaacg cggcgctcag
     1561 gccgctcgcg aacgggttct gcgagttgga cgacgagggc tgcaggccga cgaggatcga
     1621 gccgtcggcg ccgcgcaccg agctcatctc ggtctcgttc agcggctgcg gcgcggccgc
     1681 cgcgaggggg gaggcgaggc tcgcgagcag cagcgcgcag gccttgaaag cgaaggatcg
     1741 gacgtgcatc ggaggcttcc ggaagtggaa gccccaatgt gccgagcggc ccgtttttcg
     1801 atgggtggat gagggcgcgg ggagcccccg tccactcgct caagtcgggg cgtgcgcggt
     1861 cgtcgtcggc atccgccgga ggaacttgag gagcggatga accggagttc cggcccagtt
     1921 caacgcaccg gcgtggcgcg ctcgagcacg cgctcgaaca gcacgtcctc gaaacgctcg
     1981 agcggacgcg ggctgcgttg cgccttggcc agcagcgtcg cgccctgcag cgcggcgagg
     2041 acgaactcgg ccgtcccgtc gcagtcggtc gacggggcga gctcgccgcc gctcacggcc
     2101 gcgcgcaggc agaaggcgag ggcatcctgc atgtcggcca gcgcggccac gacgcggccg
     2161 cgcagcggct cgccgcagtc gttggcttcc gccgagaaat tgcccagcag gcagccgttg
     2221 cgcagcccgt cctcggcaaa gcccgtcttg ctgcgctcga tgaaggcgcg cagccgctgc
     2281 agcggcggca ggtgctcgtt gcgcagggtc tggcgcatcg ccgtgtcggc ctgcgcggcg
     2341 tagcgctcga ccacctcgag gccgaaggcc tccttggacg cgaagtggtt cgtgaacgag
     2401 ccctgcggca cgccggcggc ctgcacgatg tcgcgcacgc tggcgccacg gaacccgcgt
     2461 tcgtggacga cctgcatgcc gacctgcagc agcttctcgc gatgggaggg cttgggcatg
     2521 gcggaatact acattcgtcc ttattgccct tcaacccatg ggcgatggct catgctgccc
     2581 ggcaaggcgg cgtttgccgc gctgttccgg cctccgccgt ggcgctcgcc cgctaaagtc
     2641 gagcgcttga tgaccggccc gcgtcccgcc acggcccgcg ctcaaggaca atcgcgcccc
     2701 catggatcac accttcctct ccgcgttcat cctgctgctg ctcgtgctgg atccgttcgg
     2761 cagcctgccg atcttcatct ccatcctggg ctccgtggcg cccgagcggc gcaccttcgt
     2821 ggccgtgcgc gaggtgctga tcgcgttcgg ggtgctggtg ctgttcatgg tctcgggcca
     2881 gggcttcctc acgctgatgc ggctgtccga gcgctcgctg gaggtggccg gcggcgtgat
     2941 cctggcgatc atcgcgatcc gcatgatctt cggcggcggc gccgagatct acggcgccga
     3001 cacggccaag cgcgagccct tcatcttccc gctggccgtg ccgctgctgg ccgggccctc
     3061 ggcgatggcc accgtgctgc tgctggcctc gcgccagccc gagcacctgg tcacgtggat
     3121 cggcgcgctg accgccgcgc tggccatcag cggcgtggtg ctgctggccg ccgaccgcat
     3181 ccgccgcgtg ctcggcacgc agatggtggc ggccatcgag aagctgatgg gcctggtgct
     3241 gacggccgtg gccgtcgaga tgatcctggc cggcctgaag cgctacttca tcggcgat
//
LOCUS       DATCRO010000127         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_845222, whole genome shotgun sequence.
ACCESSION   DATCRO010000127 DATCRO010000000
VERSION     DATCRO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845222"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..2288
                     /locus_tag="VIN75_03465"
     CDS             27..2288
                     /locus_tag="VIN75_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8973250.1"
                     /translation="MWKQRVSALVGFVAWLAVALSLATHHLADAAFTTSGDGGPILNL
                     LGRPGALLSDVLLLCLGASASWLPLVGLLQSLRTLARGSRALAGVDAEAGPEPRFPRL
                     RFWGGLVLLLCASTALEWTRLYTWEARVPGGHSGGILGFALGPLSMHWLGFLGSGVLW
                     IALLVLGMSGALRFSWVRLADGIGSWIDGRREHRAVARERAEDERIGEIALREREQQV
                     DEVRIEDVQHAPIVIEPEIVQVVESRRVQRERQKPLFVEIHDNKLPQVDLLDAAPARS
                     EPTVTPESLEMTSRMIEKKLGDFGVEVRVVAAAPGPVITRYEIEPATGVKGSQIVNLA
                     KDLARSLSLVSIRVVETIPGKTTMALELPNARRQMIRLSEILGSNVYNEAGSLLTIAL
                     GKDIVGNPVVADLAKMPHCLVAGTTGSGKSVGINAMILSLLYKAEGNDVRLILIDPKM
                     LEMSVYEDIPHLLCPVVTDMKQAGNALSWCVNEMERRYKLMSKMGVRNLAGYNKKIDD
                     ARERGETIPNPFSLTPEEPEPQERLPFIVVVIDELADLMMVVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRLSFQVSSKIDSRTILDQMGAEALLGQGDM
                     LYLTPGGGVPQRVHGAFVSDDEVHRVVEYLKSQAPANYIEGILEGGVMDEDLAAGEGG
                     GSSDAESDPLYDQAVAVVLQNKRASISLVQRHLRIGYNRAARLLEQMEQSGLVSGMSG
                     SGNREILVPKRDE"
     gene            2324..2944
                     /gene="lolA"
                     /locus_tag="VIN75_03470"
     CDS             2324..2944
                     /gene="lolA"
                     /locus_tag="VIN75_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY8973251.1"
                     /translation="MTVLRSIRIPLVAALALTCASAFATATDELRDFVRTVKSGKSTF
                     TQTVTAPNGKQKLSNGSFEFSRPNRFRFVYQKPYAQTVVGDGTKVWIYDPDLNQASSR
                     KTGDALANTPAALIVSNDIDKVFTLADQPSRDGIDWVLATPRQPEGTVRSLRIGFKGH
                     ALAQLEIDDSFGQKSMIVFNGFEPNAKLAADTFVFVPPKGADVSEQ"
     gene            complement(3244..3771)
                     /locus_tag="VIN75_03475"
     CDS             complement(3244..3771)
                     /locus_tag="VIN75_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8973252.1"
                     /translation="MSAITATENRNVAQAAATAAQGAREYLSFKLGAEEYGIDILKVQ
                     EIRGYENPTRIANAAHFIKGVVNLRGVIVPIVDMRLRFGLADVQYNSFTVVIILNIAN
                     RTVGMVVDSVSDVLELGGEQIKPAPEFNGAIDAAYITGLGTIKSGDSQRMLILMDIEQ
                     MMTAPDMGLMDEPVH"
BASE COUNT          643 a         1370 c         1373 g          621 t
ORIGIN      
        1 agtccggcgc gccggtcccg cgcgcagtgt ggaagcagcg cgtctccgcg ctcgtcggct
       61 tcgtcgcgtg gctggccgtg gcgctgtctc tggccaccca ccacctggcc gacgccgcct
      121 tcaccacgtc gggcgacggc ggccccatcc tgaacctcct gggccggccg ggcgccttgc
      181 tgtccgacgt gctgctgctg tgcctgggcg cctcggcgtc gtggctgccc ctggtgggcc
      241 tgctgcagtc cttgcgcacg ctggcgcgcg gctcgcgcgc gctcgccggc gtggacgccg
      301 aggccggacc cgagccgcgc ttcccgcgcc tgcgcttctg gggcgggctc gtgctgctgc
      361 tgtgcgccag cacggcgctc gaatggacgc gcctgtacac ctgggaggct cgcgtgcccg
      421 gcgggcactc gggcggcatc ctgggctttg ccctgggccc gctgtcgatg cactggctgg
      481 gcttcctcgg ctcgggcgtg ctgtggatcg cgctgctggt gctgggcatg tcgggcgcgc
      541 tgcgcttctc gtgggtgcgg ctggccgacg gcatcggcag ctggatcgac ggccgccgcg
      601 agcatcgggc cgtcgcgcgc gaacgcgccg aggacgagcg catcggcgag atcgcgctgc
      661 gcgagcgcga gcagcaggtc gacgaggtgc gcatcgagga cgtgcagcac gcgcccatcg
      721 tgatcgagcc cgagatcgtg caggtggtcg agtccaggcg cgtccagcgc gagcgccaga
      781 agccgctgtt cgtcgagatc cacgacaaca agctgccgca ggtcgacctg ctcgacgccg
      841 cgcccgcgcg ttccgaaccc accgtcacgc ccgagtcgct ggagatgacc agccgcatga
      901 tcgagaagaa gctgggcgac ttcggcgtcg aggtgcgcgt ggtcgcggcc gcgccgggcc
      961 cggtcatcac gcgctacgag atcgagccgg ccacgggcgt caagggctcg cagatcgtca
     1021 acctcgccaa ggacctggcg cgctcgctga gcctggtcag catccgggtg gtcgagacga
     1081 tccccggcaa gacgacgatg gcgctggaac tgcccaatgc gcgccgccag atgatccggc
     1141 tgtcggagat cctgggctcc aacgtctaca acgaggccgg ctcgctgctg accatcgcgc
     1201 tcggcaagga catcgtcggc aaccccgtgg tggccgacct cgcgaagatg ccgcactgcc
     1261 tggtggccgg caccaccggc tcgggcaagt cggtgggcat caacgcgatg atcctgtcgc
     1321 tgctgtacaa ggccgagggc aacgacgtcc gcctgatcct gatcgacccg aagatgctgg
     1381 aaatgagcgt ctacgaggac atcccgcacc tgctgtgccc ggtggtcacc gacatgaagc
     1441 aggccggcaa cgcgctgtcc tggtgcgtca acgagatgga gcgacgctac aagctcatgt
     1501 ccaagatggg cgtgcgcaac ctggccggct acaacaagaa gatcgacgac gccagggagc
     1561 gcggcgagac catccccaac ccgttcagcc tcacgcccga ggagcccgag ccgcaggaac
     1621 gcctgccgtt catcgtggtc gtgatcgacg agctggccga cctgatgatg gtggtcggca
     1681 agaaggtcga ggagctgatc gcccgcctcg cgcagaaggc gcgcgccgcg ggcatccacc
     1741 tggtgctggc cacgcagcgg cccagcgtcg acgtcatcac cggcctgatc aaggccaaca
     1801 tcccgacgcg cctgtcgttc caggtgtcca gcaagatcga cagccgcacc atcctcgacc
     1861 agatgggcgc cgaggcgctc ctgggccagg gcgacatgct ctacctgacg ccgggcggcg
     1921 gcgtgccgca gcgcgtgcac ggcgcgttcg tgagcgacga cgaggtgcac cgcgtggtcg
     1981 agtacctgaa atcgcaggcg ccggccaact acatcgaggg catcctcgag ggcggcgtca
     2041 tggacgagga cctggcggcc ggcgagggcg gcgggtcatc cgatgcggag tccgatccgt
     2101 tgtatgacca ggcggtggcg gtggtgctgc agaacaagcg ggcatcgatc tcgctggtgc
     2161 agcgccacct gcgcatcggc tacaaccgcg cggcgcggct gctcgagcag atggagcagt
     2221 cggggctggt gtccggcatg tcgggctccg gcaaccgcga gatcctggtc cccaagcgcg
     2281 acgagtgagc ctccgggccc gccgtcgcag gcgaaagaca tcgatgacag tcctgcgttc
     2341 gatccgcatt cccctcgtcg ccgcgctggc gctcacgtgc gcctcggcct tcgccacggc
     2401 caccgacgaa ctgcgcgact tcgtcaggac ggtcaagtcg ggcaagtcca cgttcacgca
     2461 gaccgtcacc gcaccgaacg gcaagcagaa gctgtccaac ggcagcttcg agttctcgcg
     2521 cccgaaccgc ttccgcttcg tctatcagaa gccctacgcg cagacggtgg tgggcgacgg
     2581 cacgaaggtc tggatctacg accccgacct gaaccaggcc tcgtcgcgca agaccggcga
     2641 cgcgctcgcc aacacgccgg cggcgctgat cgtgtccaac gacatcgaca aggtcttcac
     2701 gctggccgac cagccgtcca gggacggcat cgactgggtg ctggccacgc ccaggcagcc
     2761 cgaaggcacc gtgcgctctc tgcgcatcgg cttcaagggc cacgcgctgg cgcagctcga
     2821 gatcgacgac agcttcggcc agaagtcgat gatcgtgttc aacggcttcg agcccaatgc
     2881 gaagctcgcg gccgacacct tcgtgttcgt gccgcccaag ggcgcggacg tttccgagca
     2941 gtagccgcgc ccacgacggc gcggcacgga aagatggggt caggtcttga aagttgcgcg
     3001 acaaccgctc aacgaacgcc atgcgttgcg gcgcgcaact ttcaagacct gaccccatct
     3061 aagccgttgt cgcgcaacgt tcaagacctg accccatcca ggccggatcc gcttccttga
     3121 ggcgcgcgtc cccaaagaaa acggccgcct cggttgcccg cggcggcctt cgaacccttc
     3181 gaggaaatcg tccggagtcc tggcgcggtg aggacgcgcc gaccttcgcc ccggatcaac
     3241 ggatcagtgg acgggctcgt ccatcaggcc catgtcgggg gccgtcatca tctgttcgat
     3301 gtccatcagg atcagcatgc gctgcgagtc gccgctcttg atggtgccca ggccggtgat
     3361 gtaggccgcg tcgatcgcgc cgttgaattc cggggccggc ttgatctgct cgccgcccag
     3421 ttccagcacg tcggacaccg agtccaccac catgcccacc gtgcggttgg cgatgttgag
     3481 gatgatgacg acggtgaagc tgttgtactg gacgtccgcc aggccgaagc gcaggcgcat
     3541 gtcgacgatc gggacgatca cgccgcgcag gttgacgacg cccttgatga agtgggcggc
     3601 gttggcgatg cgggtcgggt tctcgtagcc gcggatctcc tgcaccttca ggatgtcgat
     3661 gccgtattcc tcggcgccga gcttgaagga caggtattcg cgcgcgccct gcgcggccgt
     3721 ggcggccgcc tgcgcgacgt tgcggttttc ggtggcggtg atggcgctca tgggcggagg
     3781 tgctccttgc tgcggatcgg tgcgcgcggg ccgggccgaa aaagggcagg ccgcgccgat
     3841 gccatgcagt gtgaagccgc gccgccccgc cgaatggccg aaaagaccgc cgcggcggcg
     3901 ccaattccaa ggattccacg aatgtgccgg gcaaatcggc ggggatgtcc gccgctgtac
     3961 gatcacggac tcccatggcc gacctcttct ccacgaaacc cgtcccg
//
LOCUS       DATCRO010000128         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_46423, whole genome shotgun sequence.
ACCESSION   DATCRO010000128 DATCRO010000000
VERSION     DATCRO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46423"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VIN75_03480"
     CDS             complement(<1..547)
                     /locus_tag="VIN75_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8973253.1"
                     /translation="MSHRYLSGVWIPIVTPFREGRVDHDALATLARRLAADGVDGIVV
                     CATTGEAPLLGDAERVEVLGTIKSATTLPLIMGSSGSTAAEVLRRADAAMAHAPAAFM
                     VTAPPYLRPSQDALRAFFTTVADGLPAPLVVYDIPARTGVRIELDTLLALAAHPRVVA
                     LKDCGGRVEQTEALIADGRLQV"
     gene            862..1191
                     /locus_tag="VIN75_03485"
     CDS             862..1191
                     /locus_tag="VIN75_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8973254.1"
                     /translation="MNPADPVQRQLEAYNARDLARFVAEYSDDVQVYKPPAAKPVLSG
                     KAAFAAHYASNRFNLPGLHATVVARMVSGNTVVDHEHVAGLKDGIVEAIAVYRVDGGR
                     ITTVWFY"
     gene            complement(1198..2223)
                     /locus_tag="VIN75_03490"
     CDS             complement(1198..2223)
                     /locus_tag="VIN75_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8973255.1"
                     /translation="MEQRRLGASGLMVPALSLGTGTFGGGNAFFAAWGSTDADGARNL
                     VDICLEHGLTMFDTADVYSSGIAEQVLGKAIAGRRDQVLVSTKATFRSGPGPNDVGSS
                     RQHLRAAVEGSLRRLGTDYVDLFQLHGFDALTPVEETLSTLDELVREGKIRYLGVSNF
                     SGWHLMKSLAASERLGLQRYVAHQAYYSLVGRDYEWELMPLGLDQGVSAVVWSPLGWG
                     RLTGKIRRGSGIPAQSRLHATADKGPPVDDEHLFKVVDALAAVGEEIGKSVPQVALNW
                     LLQRPTVANVIVGARDEKQLRENLGAVGWNLSAEQVRRLDEASAVTRPYPYWHQEGFT
                     ERNPLPV"
     gene            2447..3337
                     /locus_tag="VIN75_03495"
     CDS             2447..3337
                     /locus_tag="VIN75_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973256.1"
                     /translation="MTAASIVRLLSLSAIWGASFLFMRMGAPVLGPTLLIFCRVLSAA
                     LFLAIVGAAMRKPLEPRRHALHFLVLGLFNSALPFLLFAFAARTLSASLLSILNATAP
                     IWGAVIGAAWTRTAIAPKALLGLALGVGGVALLVGFDPGSLRPGAPLAIALGLGATLS
                     YGIATNYAKSARKVDAFANAHGSMWAATLLVAPAVPFAPAAAAPTLGIGLAVLALGVV
                     CSGIAYLLYFRLVADLGAASALTVTFLVPVFGVLWGHLFLGEATGWNTWVGAAIVIAG
                     TALVTGFSPGAVFRRAPAAA"
     gene            3462..>4049
                     /locus_tag="VIN75_03500"
     CDS             3462..>4049
                     /locus_tag="VIN75_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973257.1"
                     /translation="MTAATRADAQSRVDDILTFRKELARVEAEGALRLAGDQRSALQH
                     HHDALLAALHAGFDVDRDVRARQLSLGMRIASLIGALALAASVFFLFWNFWGRLSTPV
                     QVTTLVAFALGSFLATTAIARRDANGYFTKLAALVAFACYVLDVVLLGRIFDIAPSDN
                     AWLAWAAYAFLLAYAHELRLLLVAGIVSLVAFIAAR"
BASE COUNT          520 a         1465 c         1431 g          633 t
ORIGIN      
        1 gcacctgcag gcggccgtcg gcgatcaggg cctcggtctg ctcgacgcgg ccgccgcagt
       61 ccttcagggc caccacgcgt ggatgcgcgg ccagcgcgag cagcgtgtcg agctcgatgc
      121 gcacgccggt gcgcgcgggg atgtcgtaga ccaccagcgg cgccggcagg ccgtcggcga
      181 cggtggtgaa gaaggcgcgc aacgcgtcct gcgacgggcg caggtagggc ggcgccgtca
      241 ccatgaaggc ggccggtgca tgcgccatcg cggcgtcggc gcggcgcagc acctcggccg
      301 cggtgctgcc cgaggaaccc atgatcagcg gcagcgtcgt ggccgacttg atcgtgccca
      361 gcacctcgac gcgctcggca tcgcccagca gcggcgcctc gcccgtggtg gcgcacacga
      421 cgatgccgtc gacgccgtcg gcggcgagcc gcctggcgag cgtggcgagc gcgtcatgat
      481 cgacgcgtcc ctcgcggaac ggggtgacga tggggatcca gaccccggac agatagcgat
      541 ggctcacgca gctttccttt cgacaacgac cggacaatga ccgtcgttgg aggagcgcgc
      601 agagcaggcc ggggttccgt gaaggtttct tccgcgggag ggcaggttcc gtcgctcatc
      661 tggacgacgg aaccgcagcc ccggtcagac gagcaacggc tttttcactt tgaccgcacc
      721 ggcgcgcgcg ggcgtcacgc gcgtgggcgc gtgagcggcg atcgtgacga cgaggcgggt
      781 catgccccga tcttagccga cgcgagtcgc cgcggaaggt tcagaatccc gcacgacgct
      841 cctgacatcg cgagacccgc catgaacccc gccgacccgg tccaacgcca gctcgaggcc
      901 tacaacgcac gcgacctcgc gcgcttcgtg gcggagtaca gcgacgacgt gcaggtgtac
      961 aagccgcccg ccgcgaagcc ggtgctctcg ggcaaggcgg cgttcgcggc ccattacgcc
     1021 agcaatcgct tcaacctgcc cggcctgcac gcgaccgtcg tcgcgcgcat ggtcagcggc
     1081 aacacggtgg tcgaccacga gcacgtcgcc gggctgaagg acggcatcgt ggaggcgatc
     1141 gccgtctacc gcgtcgacgg cggacgcatc accaccgtct ggttctactg aggcggttca
     1201 caccggcagc gggttgcgct cggtgaagcc ttcctggtgc cagtacggat agggccgcgt
     1261 caccgcgctc gcctcgtcga gccttcggac ctgctcggcg ctgaggttcc agccgacggc
     1321 gcccaggttc tcgcgcagct gcttctcgtc gcgcgcgccc acgatcacgt tggcgaccgt
     1381 cgggcgctgc agcagccagt tcagcgccac ttgcggcacc gacttgccga tctcctcgcc
     1441 gacggcggcg agcgcgtcca ccaccttgaa caggtgctcg tcgtcgaccg gcgggccctt
     1501 gtcggcggtc gcgtgcagcc ggctctgcgc cgggatgccc gagccgcggc ggatcttgcc
     1561 cgtgagccgg ccccagccca gcgggctcca gacgacggcc gagacgccct ggtccaggcc
     1621 cagcggcatc agctcccatt cgtagtcgcg gcccaccagc gagtagtagg cctggtgcgc
     1681 gacgtagcgc tgcaggccca gccgctccga cgcggcgagc gacttcatca ggtgccagcc
     1741 cgagaagttg gacacgccga ggtagcggat cttcccttcg cgcacgagct cgtcgagcgt
     1801 ggacagcgtc tcctcgaccg gggtcagcgc gtcgaagccg tgcagctgga acaggtcgac
     1861 gtagtcggtg cccaggcggc gcagcgagcc ctcgaccgcc gcgcgcaggt gctggcgcga
     1921 cgacccgacg tcgttcgggc cggggccgct gcggaaggtc gccttggtcg agacgagcac
     1981 ctggtcgcgc cggcccgcga tcgccttgcc cagcacctgc tcggcgatgc cgctcgagta
     2041 gacgtcggcg gtgtcgaaca tcgtcaggcc gtgctccagg cagatgtcga ccaggttgcg
     2101 cgcgccgtcg gcgtcggtcg agccccaggc cgcgaagaag gcgttgccgc cgccgaaggt
     2161 gccggtgccc aggctgagcg cgggcaccat gaggccggag gcgccgagtc gtcgttgttc
     2221 catgtgatct cctgcgaagg gttgggacgg gcgcattctc gagacgcggc ccggtgccga
     2281 cgcgccctgt ccggacatcg aaatgtacgg actccttccc gtcgggcgcc ggccggagac
     2341 cctgcggcga acgcggtgtc gattggccat ttccatgtcc gaagatggcg tgttccgacc
     2401 ggcgcgcctc gcgacaatcg tccccgaccc aacgacttca tcgcccatga ccgccgccag
     2461 catcgtccgc ctgctcagcc tgtccgccat ctggggcgcc tcgttcctgt tcatgcgcat
     2521 gggcgcgccg gtgctcgggc cgacgctgct gatcttctgc cgcgtgctgt cggcggcgct
     2581 gttcctggcg atcgtcggcg ccgcgatgcg caagccgctc gagccacgcc gccacgcgct
     2641 gcacttcctg gtcctgggcc tgttcaactc ggccctgccc ttcctgctgt tcgcgttcgc
     2701 cgcgcgcacc ctgtcggcct cgttgctgtc catcctcaac gccaccgcgc cgatctgggg
     2761 cgccgtgatc ggcgccgcgt ggacgcgcac cgccatcgca ccgaaggcgt tgctcggcct
     2821 cgcgttgggg gtgggcggcg tcgcgctgct cgtcggcttc gatcccggga gcctgcgacc
     2881 cggcgcgccg ctggcgatcg ccctcgggct gggcgccacg ttgtcgtacg gcatcgccac
     2941 caactatgcg aagtcggcca ggaaggtgga cgcgttcgcc aacgcgcacg gcagcatgtg
     3001 ggccgcgacg ctgctggtcg cgccggccgt gccgttcgca cccgcggccg ccgcgccgac
     3061 gctcgggatc ggcctggccg tgctcgcgct cggcgtcgtg tgcagcggca tcgcctacct
     3121 gctgtacttc cggctggtgg ccgacctcgg cgcggcctcc gcgttgacgg tcaccttcct
     3181 cgtcccggtg ttcggcgtgc tgtggggcca cctgttcctg ggcgaggcga ccggctggaa
     3241 cacctgggtg ggcgccgcga tcgtcatcgc gggcacggcg ctggtgacgg ggttcagccc
     3301 gggcgcggtg ttcaggcgcg cccccgccgc ggcctgagct gggcgcccgg cggtatcgcg
     3361 cgccgattcc gccgggccag cgagccgaca ccggcgcgct cgcgctggca cgcggcggcg
     3421 gcctgggcca tgctcgtggc tccgttgcaa tggccccctc catgaccgct gccacccgag
     3481 ccgacgcaca atcgcgcgtc gacgacatcc tgaccttccg caaggagctc gcgcgcgtcg
     3541 aggccgaagg cgccctgcgc ctggccggcg accagcgatc cgcgctgcaa caccaccacg
     3601 acgcgctgct ggcggcgctg cacgcgggct tcgacgtcga tcgcgacgtg cgtgcgcggc
     3661 agctgtcgct gggcatgcgc atcgcgtcgc tgatcggcgc gctggcgctg gcggccagcg
     3721 tgttcttcct gttctggaac ttctggggcc gattgtcgac gcccgtgcag gtgacgacgc
     3781 tggtcgcctt cgcgctcggc tccttcctgg ccacgacggc catcgcgcgg cgcgacgcga
     3841 acggctactt caccaagctc gcggcgctgg tggcgttcgc gtgctacgtg ctggacgtgg
     3901 tgctgctggg ccggatcttc gacatcgcgc cgtccgacaa cgcgtggctc gcctgggccg
     3961 cctacgcgtt cctgctggcc tatgcccacg agttgcggct gctgctggtg gcgggcatcg
     4021 tgtcgctggt ggccttcatc gcggcccgc
//
LOCUS       DATCRO010000129         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_451485, whole genome shotgun sequence.
ACCESSION   DATCRO010000129 DATCRO010000000
VERSION     DATCRO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451485"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VIN75_03505"
     CDS             complement(<1..741)
                     /locus_tag="VIN75_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973258.1"
                     /translation="MANKAKLHEILAVEKSRTGQVKTLLDDTSRKFNRATDYFSGHLK
                     SLKLIRDDPANAAIEAAAREQRQLPTTVVETLDYLFKHWANAEDVLLQKSLTNQVAKA
                     DIVLSDGKTLASNVPVDELLGLESRLEELRKLFHAMPTNRADTRYAPSDDGRKGSWTA
                     VDADVTTKTERTKEPFIKAPATDKHPAQVDVVEKDVVVGTFTNIKLSGAATTLQKALA
                     IELVDDLIASTKQARMRANTVEVLEGRVG"
     gene            complement(921..1694)
                     /locus_tag="VIN75_03510"
     CDS             complement(921..1694)
                     /locus_tag="VIN75_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8973259.1"
                     /translation="MTRALIADDEQPLAHYLRDQLARLWPGLDVVAIARNGLEAAAQI
                     AELQPDIAFLDIQMPGLTGLEVAAGIEGLTRVVFVTAYDEYALPAFDAAALDYVLKPV
                     TAERLGRTVERLQRALAPPAAPGDAASADDDRLARVLRALQAPVAAAPPLRWIRASQG
                     ELTHQVPVADAMFFHADDKYTCVRTAKAEYLIRTSITELAAQLDPGQFWQIHRSTIVN
                     LAHLAGTRRDEASRLFVRIAGWKDELPVSRAYVHLFKPM"
     gene            complement(1725..2909)
                     /locus_tag="VIN75_03515"
     CDS             complement(1725..2909)
                     /locus_tag="VIN75_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8973260.1"
                     /translation="MDNGHPTSIAELRSMWQAWLSGTSPVQPPLWVQFAWTVAFSAVV
                     AFGFTVLFFIANADTIAKWLDLGEWARWYGRNLVVSLTVGVVIAILFRVAFAMFGARR
                     LNGLAGWRRTAFFSGVPLLGTAIGWPIGMTLIFGEQRLFHGMTSGNVVATVVLAIVIS
                     LGFQTYFSLRTRGIRAEMAASEAQLRLLQGQMEPHFLFNTLANVICMIDTDAPRAKHT
                     LEALTDYLRASLGSLRVDDSTLGAELDLAGRYLELMQARMGERLRFELQADDALRHAA
                     LMPLVLQPLIENAVKHGLEPQVDGGTVRVKAARLPVDGHDSLQVCVEDDGAGLAAAAS
                     RPRRRVAGGADGNGIALANLRERLRSRFGPAAQLTLSELEDGHGTRACLVIPWLDARA
                     PA"
     gene            3085..4473
                     /locus_tag="VIN75_03520"
     CDS             3085..4473
                     /locus_tag="VIN75_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY8973261.1"
                     /translation="MQVQASAFKAYDIRGIVDQALDETFAEHLGRAFGSEAVRLGEKA
                     VAVGRDGRVSGPRLVAALIKGLASTGLDVVDLGAVTTPMLYYVAATRGKHGCHSGIQV
                     TGSHNPKDWNGFKMVLAGRAIHGADIQQLRTLMESETYVSGTGRVGRMDILAEYTHRI
                     ARDCKLARPMKIVVDSGNGIPGASAPGILRALGCEVIDLYSRVDGDFPNHHPDPSKPE
                     NLVDLIRTVHATEAELGLAFDGDGDRLGVVTRSGSIIYPDRQLMLFARDILTRVKGGR
                     IIYDVKCSQRLPVAIREAGGEPLMWKTGHSLVKAKLKETGAPIAGEMSGHIFFAERWY
                     GFDDATYTAARLLEILSRSADPSAVLEELPTSFSTPELNVACAEGEPPRIIERLLAEA
                     KFPGAEILTIDGLRAEYEDGFGLIRASNTTPVLVLRFEGHTQAALERIQHDFMQALRK
                     VKPDAHVGAAAH"
     gene            4480..>4962
                     /locus_tag="VIN75_03525"
     CDS             4480..>4962
                     /locus_tag="VIN75_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8973262.1"
                     /translation="MPRQSMTLKQRAALAAYTLALRLLLPAILVRYWLRGRKEPGYRV
                     AMRERLGCGPQVDPGSVWLHAVSLGETRAATALVEALRARRPDMRLLLTHGTATGRDA
                     GQALMRHGDRQSWLPLDTPGAVRRFLLRTRPALGVLMETEIWPNLLHGAEERGVPMVL
                     A"
BASE COUNT          712 a         1741 c         1796 g          713 t
ORIGIN      
        1 gccgacgcgg ccctcgagca cctcgacggt gttcgcgcgc atgcgggcct gcttggtcga
       61 ggcgatcagg tcgtcgacca gctcgatggc cagggccttc tgcagcgtgg tggccgcgcc
      121 cgagagcttg atgttggtga aggtgccgac caccacgtcc ttctcgacga cgtcgacctg
      181 cgccggatgc ttgtcggtgg caggcgcctt gatgaagggc tccttggtgc gctccgtctt
      241 ggtcgtgacg tcggcgtcga ccgccgtcca ggatcccttg cggccgtcgt cggacggcgc
      301 gtaccgggtg tcggcccggt tggtcggcat ggcgtggaaa agcttgcgca gttcctcgag
      361 ccggctctcg agcccgagca gttcgtcgac cgggacgttg ctggcgagcg tcttgccgtc
      421 ggacagcacg atgtcggcct tcgccacctg gttggtcagg gacttctgca gcagcacgtc
      481 ctccgcgttc gcccagtgct tgaacaggta gtccagcgtc tcgaccaccg tggtgggcag
      541 ctggcgctgc tcgcgcgcgg cggcctcgat ggccgcgttg gccgggtcgt cccggatcag
      601 tttcagggat ttcaggtggc ccgagaagta gtccgtcgcc cgattgaact tgcgcgaggt
      661 gtcgtcgagc aaggtcttga cctgtcccgt gcgcgacttc tcgacggcga ggatctcgtg
      721 cagctttgcc ttgttggcca tgtcgtggtg tccctggggg tgatgtgtcg ttcgatcgag
      781 gcgtgcgcgc tcggcgccgc cttccacatc gccactgtag gagcgggcgc caggaacacc
      841 caacggcatt cggcccggtt aacccgccga atttccgcgc acgggcgggg acagcgcgcc
      901 ggccgagcgt ccgagcgcca tcacatcggc ttgaacaggt gcacatatgc cctgctcacc
      961 ggcagctcgt ccttccatcc cgcgatgcgc acgaacaggc ggctggcctc gtcgcggcgc
     1021 gttcccgcca ggtgggccag gttgacgatc gtggagcggt ggatctgcca gaactgcccg
     1081 ggatcgagct gcgcggccag ctcggtgatc gaggtgcgga tcaggtattc ggccttggcg
     1141 gtgcgcacgc acgtgtactt gtcgtcggcg tggaagaaca tcgcgtcggc gaccggcacc
     1201 tggtgcgtca gttcgccctg gctggcgcgg atccagcgca gcggcggcgc ggccgccacg
     1261 ggtgcctgca gcgcgcgcag cacgcgggcg aggcggtcgt cgtcggcgga ggcggcatcg
     1321 cccggcgcgg cgggcggggc gagcgcgcgc tgcaggcgtt cgaccgtgcg ccccaggcgt
     1381 tcggcggtga cgggcttgag cacgtagtcc agcgccgcgg cgtcgaacgc aggcagcgcg
     1441 tactcgtcgt aggcggtgac gaagacgacg cgcgtcaggc cctcgatgcc ggcggccacc
     1501 tccaggccgg tcaggccggg catctggatg tcgaggaacg cgatgtcggg ctgcaactcg
     1561 gcgatctgcg cggccgcctc caggccgttg cgcgcgatgg cgacgacatc gaggccgggc
     1621 cacagcctgg ccagctggtc gcgcaggtag tgggccaggg gttgctcgtc gtcggcgatg
     1681 agggcgcggg tcatggtggg gcgtcgcgag aggtggtcgg attgtcaggc gggggcgcgg
     1741 gcatcgagcc aggggatcac gaggcaggcg cgggtgccgt ggccgtcctc gagctcggac
     1801 agcgtgagct gtgcggcggg gccgaagcgg gaacgcaggc gctcgcgcag gttggccagc
     1861 gcgatgccgt tgccatcggc gccgccggcc acgcggcggc gcgggcggct cgcggcggcg
     1921 gccaggccgg cgccgtcgtc ctcgacgcag acctgcagcg agtcgtggcc gtcgacgggc
     1981 aggcgcgccg ccttcacgcg cacggtgccg ccgtcgacct gcggctcgag cccgtgcttg
     2041 accgcgttct cgatcagcgg ctgcagcacc agcggcatca gcgcggcgtg gcgcagggcg
     2101 tcgtcggcct ggagctcgaa gcgcagccgc tcgcccatgc gcgcctgcat cagctccagg
     2161 tagcgaccgg ccaggtccag ctcggcgccc agcgtgctgt cgtcgacccg caggctgccc
     2221 agcgacgcgc gcaggtagtc cgtcagcgcc tccagcgtgt gcttggcgcg cggggcgtcg
     2281 gtgtcgatca tgcagatgac gttggccagc gtgttgaaca ggaaatgcgg ctccatctgg
     2341 ccctgcagga ggcgcagctg cgcctcgctg gcggccatct cggcgcggat gccgcgcgtg
     2401 cgcagcgaga agtaggtctg gaagcccagc gagatcacga tggccagcac gacggtcgcc
     2461 accacgttgc ccgaggtcat gccatggaac aggcgctgct cgccgaagat cagggtcatg
     2521 ccgatcggcc agccgatggc ggtgcccagc agcggcacgc ccgagaagaa cgccgtgcgg
     2581 cgccagccgg cgagcccgtt gagccggcgc gcgccgaaca tcgcgaaggc caccctgaac
     2641 aggatcgcga tgaccacgcc gacggtcagc gacaccacca ggttgcggcc gtaccagcgc
     2701 gcccattcgc ccaggtccag ccatttggcg atcgtgtcgg cgttggcgat gaagaacagc
     2761 acggtgaagc cgaacgcgac gacggccgag aaggcgaccg tccacgcgaa ctgcacccac
     2821 agcggcggct gcaccgggga cgtgccggaa agccaggcct gccacatgct gcgcagctcg
     2881 gcgatcgagg tggggtgccc gttgtccatg gcttgccagt gtaggcaccc cggcgcgggc
     2941 ggccagcggg acgcgacggc tggccggcgg cgccgacgga tcgacgcccg gcggcgcgga
     3001 cggtgccccc gcgaccgggg gcgggaaccg ccgggcaccc ctcgtagaat cgcgagccaa
     3061 cgcccaccca tcccgaacca tcccatgcaa gtccaggcat ccgccttcaa ggcctacgac
     3121 atccgcggca tcgtcgacca ggccctcgac gagaccttcg ccgagcacct gggccgggca
     3181 ttcggcagcg aggcggtgcg cctcggcgag aaggccgtgg cggtcggccg cgacggccgc
     3241 gtgtcgggcc cgcgcctggt ggccgcgctg atcaagggcc tggcatcgac cgggctggac
     3301 gtcgtcgacc tgggcgccgt caccacgccg atgctgtact acgtggccgc cacgcgcggc
     3361 aagcacggct gccactctgg catccaggtc accggcagcc acaacccgaa ggactggaac
     3421 ggcttcaaga tggtgctggc cggccgcgcg atccacggcg cggacatcca gcagctgcgc
     3481 acgctgatgg aaagcgagac gtacgtgagc ggcacgggcc gcgtcggcag gatggacatc
     3541 ctggccgagt acacccaccg catcgcccgc gactgcaagc tcgcgcggcc gatgaagatc
     3601 gtcgtcgatt cgggcaacgg cattcccggc gcgtccgcgc ccggcatcct gcgcgcgctc
     3661 ggctgcgagg tgatcgacct gtacagccgc gtcgacggcg acttccccaa ccaccacccg
     3721 gacccgtcca agcccgagaa cctggtcgac ctgatccgca ccgtgcatgc caccgaggcc
     3781 gagctgggcc tggccttcga cggcgacggc gaccgcctgg gcgtcgtcac gcgcagcggc
     3841 agcatcatct atccggaccg ccagctgatg ctgttcgcgc gcgacatcct cacgcgggtc
     3901 aagggcggcc ggatcatcta cgacgtcaag tgctcgcagc gcctgccggt ggccatccgc
     3961 gaggccggcg gcgagccgct gatgtggaag acgggacact cgctggtcaa ggccaagctc
     4021 aaggagaccg gcgcgcccat cgccggcgag atgagcggcc acatcttctt tgccgagcgc
     4081 tggtacggct tcgacgacgc cacgtacacc gcggcgcgcc tgctcgagat cctctcgcgc
     4141 agcgcggacc cgagcgcggt gctcgaggag ctgcccacca gcttcagcac gcccgaactc
     4201 aacgtggcct gcgccgaggg cgagccgccg cgcatcatcg agcgcctgct cgccgaggcg
     4261 aagttccccg gcgccgagat cctgaccatc gacggcctgc gcgccgaata cgaggacggc
     4321 ttcggcctga tccgcgcgtc caacaccacg ccggtgctgg tgttgcgttt cgagggccac
     4381 acgcaggccg cgctggagcg catccagcac gacttcatgc aagccctgcg caaggtcaag
     4441 cccgacgcgc acgtcggcgc cgcggcgcac tgagcctgca tgccccggca gtccatgacc
     4501 ctcaagcagc gcgccgcgct cgccgcgtac acgctcgcgc tgcgcctgct gctgccggcc
     4561 atcctcgtgc gctactggct gcgcggccgc aaggagccgg gctatcgcgt cgcgatgcgc
     4621 gagcgcctgg gctgcggtcc gcaggtcgac ccgggctcgg tctggctgca cgccgtctcc
     4681 ctcggcgaga cccgcgccgc gaccgcgctg gtcgaggcgc tgcgcgcgcg ccggcccgac
     4741 atgcggctgc tgctgacgca cggcaccgcc accggccgcg acgccggcca ggccctgatg
     4801 cgccatggcg accgccagtc ttggctgccg ctggacacgc ccggcgccgt gcgccgcttc
     4861 ctcttgcgca cgcggcccgc gctgggcgtg ctgatggaga ccgagatctg gccgaacctg
     4921 ctgcacggcg ccgaggagcg cggcgtgccg atggtgctgg cc
//
LOCUS       DATCRO010000130        10066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1115087, whole genome shotgun sequence.
ACCESSION   DATCRO010000130 DATCRO010000000
VERSION     DATCRO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115087"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..747)
                     /locus_tag="VIN75_03530"
     CDS             complement(82..747)
                     /locus_tag="VIN75_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973263.1"
                     /translation="MPRLHRFLAALLLAPAALLAAPGAGFRALRVADPVGGDSMPGYV
                     FYPTDQAGKDTRIGPYDVAATLDAPPRAGPKPLVVLSHGNAGSALGHHDLAAFLATHG
                     FVVATIEHPRDNFHDTSGVGQAAVLAGRPIQVKATISTLLADPQWKSLVDPGRIGVAG
                     FSAGGYTSLLLVGAVPRFRRFIDYCQRYAEDALCAEAPRIEAEARRRAAPSSSSWTTS
                     RGS"
     gene            complement(807..1778)
                     /locus_tag="VIN75_03535"
     CDS             complement(807..1778)
                     /locus_tag="VIN75_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973264.1"
                     /translation="MGFDATRRDVLGLLGAALSLPALAAAPALEAPPGQDGFLDLMPE
                     FWMAYDAHRAEAAPARTQALVDGFFSKHADRYLQAGVKARPEDVAKWLPRFDGLATAA
                     RGVHRRFAHGYAGNLGRFRSVLADFDGRASPVTLMPSLMHFDAHLQPDGPRLPLFFGP
                     DAIVYLHGADADLAVLFSHELFHCYQGQKNPAMCLDPRAPVYASLWMEGTATWASECM
                     NPGASPQAVLLNAELAQATPSQLHAAARAMLSALDAWDDATQSLFFSIGQHGDGWPPR
                     AGYAVGLQIARGLGKTMSLPQMAALPAPRVRETLAQALADFVKAPPG"
     gene            complement(1852..2859)
                     /locus_tag="VIN75_03540"
     CDS             complement(1852..2859)
                     /locus_tag="VIN75_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY8973265.1"
                     /translation="MNIIILDDYQDAVRKLPCAAKLSELNAKVFTNTVKGIGQLSVRL
                     RDAEVLVLIRERTRFPRQLLEKLPKLRLIAQTGKVGEHIDIEACTQQGIAVAEGIGSP
                     YAPAELTWALIMASMRRLPHYIASLKHGAWQQSGLKAASMPPNFGIGTTLRSKTLGIW
                     GYGRIGKLVAGYGKAFGMEVAVWGSEESRARAEADGHHALASREELFASADVLSVHLR
                     LTESTRHLIKLNDLLQMKPTALFVNTSRAELIEEAALVAALNRGRPGMAAVDVFESEP
                     ILAGHPLLRLENAVCTPHIGYVEQSSYEMYFGAAFDNVVNFINGTPTNIVNPDALKVL
                     R"
     gene            complement(2958..3392)
                     /locus_tag="VIN75_03545"
     CDS             complement(2958..3392)
                     /locus_tag="VIN75_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973266.1"
                     /translation="MSTSLRYDPLGLSELDSRLAPESIDFVYEPAFASDSAFPDAVAL
                     PASRGDRIESRRAFVEMKQLFMSAVENLAHRKGAWLRAQVRAAEDPVDLWLLRGPLLS
                     ALREDELATRAMRAALYRSLDRIFPETFGSAASGLLPAPTRF"
     gene            complement(3382..3813)
                     /locus_tag="VIN75_03550"
     CDS             complement(3382..3813)
                     /locus_tag="VIN75_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973267.1"
                     /translation="MSTALLQRLLHRKAAPPTTLRMNVCPPDVVPRRDSLWTATLRWL
                     VGGDVEVVPALRTPLERARAEFVGALAGLQEVDPHGLLKRAQHARSLRELWHLRSELY
                     TLIARRVSQVEADVRLARVNQHFPTRAQRSSAPAAEIADVH"
     gene            complement(3936..4874)
                     /locus_tag="VIN75_03555"
     CDS             complement(3936..4874)
                     /locus_tag="VIN75_03555"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY8973268.1"
                     /translation="MKARAIALAAALLLAAAARAQTPTLKLVDDAGHELALQHPAQRI
                     VALAPHLTELVYAAGAGGKLVAVGKYSDFPPDALAKPVISDAFAVNYEALAGLKPDLV
                     LVWGSGTPERIKAKLRTLGVPVYEIEIRTVAGLVDTLRRIGRLAGTEGVAQARAQAIA
                     ADWAALKATYAGRRPVRVFYQLWDAPLMTLNGQHLVSSAIAACGGSNVFAELPTLTAT
                     VSWEVAVQRDPELVVTAGAPGEPGKPGRWTQFKRVSAVQHAQFGHVEGDLLDRSGPRF
                     VDGARELCEAIDHARQGLPQPVFGTPRPQGAASAGR"
     gene            complement(4871..5575)
                     /gene="nth"
                     /locus_tag="VIN75_03560"
     CDS             complement(4871..5575)
                     /gene="nth"
                     /locus_tag="VIN75_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8973269.1"
                     /translation="MNEKDIDTFFATLQAANPSPASELHYASVFELLTAVLLSAQATD
                     AGVNKATRTLFVAAPTPARMLALGTAGVEAHVRTIGLYRNKTKHLLETCRILIEQHGG
                     QVPRDRAALEALPGVGRKTANVILNVAFGEPTLAVDTHVFRVANRTGLAPGKNPLAVE
                     LKLLERVPEKYLDDAHHWLILHGRYVCLARMPQCGRCAVTQTCDWFKALPPQARPRPG
                     QAAPDGKALRAAGIEP"
     gene            complement(5699..6685)
                     /locus_tag="VIN75_03565"
     CDS             complement(5699..6685)
                     /locus_tag="VIN75_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973270.1"
                     /translation="MTQPVQAEASTFDDVVLPSQTAVPLTRARSNLILLGVSLIWASA
                     FVAQAVAARHLGASTFTGLRFALGAAVVAPLAWREWHMLRARRREPRRRDLAHIAGLG
                     TLLFLGAAMQQMGMATTTVTNTGFLSALYIPLVPLLAWVASRQAPHWTTWLAAAGCVV
                     GSWLLTGGGSLSAFRTGDWWIIASSLPWSVHVMLVGRAANRLHGAMLVACGQFIACSV
                     LALAFGLATEPLSADGFRQALWPIAYTGVLSVGLGFTLQVVAQRHTRPADSAIVLSSE
                     TVFTAAFGAVFMGDRMGAAGLAGCALILACVLMVQLQPIVWRWFADSGSKAA"
     gene            complement(6682..7371)
                     /gene="rsxB"
                     /locus_tag="VIN75_03570"
     CDS             complement(6682..7371)
                     /gene="rsxB"
                     /locus_tag="VIN75_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEY8973271.1"
                     /translation="MSPTVTINLQTATPPAGVASLAQRLDALLPQTQCTRCGYPDCRS
                     YAEALAAGEARLNQCPPGGAEGIARLARELGQPELPLDPAFGDEAPRRIVYIEEAACI
                     GCTLCIQACPVDAIIGAPKRMHAVVEADCTGCELCLPVCPMDCIALEEASAGATGWDA
                     WSSARADTARDRFARRQARLARDAREHDARLSAKAEAPADVEAAPEDPKRAAVAAALA
                     RARLRAKARAQ"
     gene            complement(7380..8750)
                     /gene="phaZ"
                     /locus_tag="VIN75_03575"
     CDS             complement(7380..8750)
                     /gene="phaZ"
                     /locus_tag="VIN75_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY8973272.1"
                     /translation="MLYQLYEAQRALMAPFADFAAATAKLYKHPLSPFTHIPGAQRLS
                     AGFELMHRLGMDYEKPEFEIKNVKSGGVDVAVQEQVTLEKPFCRLIRFKRYSDDSATL
                     DRMKAQPSVLVVAPLSGHHSTLLRDTVRSLLQDHKVWITDWTDARMVPVEAGPFHLDD
                     YIRYVQEFIRLIGADVHVISVCQPTVPVLAAVSLMASAGETTPLSLTMMGGPIDARNS
                     PTAVNNLAMNRSYEWFENNVIHRVPPNYPGAQRQVYPGFLQHTGFVAMNPDRHATAHW
                     DYFLDLVRGDDDSTEAHRKFYDEYNAVLDLPAEYYLDTIKTVFQDFSLVKGTWDVDGH
                     LVRPQDIHSSALLTIEGELDDISGAGQTRAAHGLCTGIPKTRQFHYDVEGAGHYGIFS
                     GRRWRDIVYPHVRDFIARFEQERAGRPAKAPTAARTSRAIATSAAAKSPTKARRTVKV
                     TPPAKG"
     gene            complement(9165..>10066)
                     /locus_tag="VIN75_03580"
     CDS             complement(9165..>10066)
                     /locus_tag="VIN75_03580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY8973273.1"
                     /translation="QALGGTGGLKVGADFLKRLRPDAKVLISDPSWENHRALFTNAGF
                     AVEQYPYYDAATRGVAFDAMIAKLASSPAGTIVVLHACCHNPTGVDLSAEQWTKVIAT
                     IREHGLVPFVDMAYQGFGFGIAEDGAAVRQFMASGLDFFVSTSFSKSFSLYGERVGAL
                     SVVCSSKEEAARVLSQLKIVIRTNYSNPPTHGAQIVATVLTTPALRAQWESELAGMRD
                     RIKRMRGALVDQLTNAGVKGDLSYITRQNGMFSYSGLSAAQMQRLRSEFGVYGVDSGR
                     ICVAALNDHNLAYVAKAIAAVSA"
BASE COUNT         1432 a         3431 c         3739 g         1464 t
ORIGIN      
        1 ccgccgcatc gaagaccagg ctgagcggcg ccatcgcgaa cgcggccttg acgcgcgggt
       61 cggacggcgg gccccagcgg gtcagctgcc cctggatgtc gtccatgagc tgctcgaggg
      121 tgcggccctg cgcctggcct cggcctcgat gcgcggcgcc tccgcgcaga gcgcgtcctc
      181 cgcgtagcgc tggcaatagt cgatgaagcg ccggaatcgc ggcacggcgc ccaccagcag
      241 caggctcgtg tagccgccgg ccgagaagcc cgccacgccg atgcgccccg gatcgaccag
      301 gctcttccac tgcgggtcgg ccagcagcgt gctgatcgtc gccttcacct ggatcggccg
      361 gcccgcgagc accgcggcct ggcccacgcc gctggtgtca tggaagttgt cgcgcgggtg
      421 ctcgatggtc gccacgacga agccgtgcgt cgcgaggaag gcggccaggt cgtggtggcc
      481 cagcgccgag ccggcgttgc cgtgcgacag gacgaccagc ggcttcgggc cggcacgcgg
      541 cggggcatcg agcgtggccg ccacgtcgta ggggccgatg cgcgtgtcct tgcccgcctg
      601 gtcggtcggg tagaacacgt acccaggcat gctgtcgccg cccacggggt cggcgacccg
      661 cagcgcgcga aagcccgcgc cgggcgccgc cagcagggcg gcgggcgcga gcagcagggc
      721 ggccaggaat cggtgcaggc gtggcatggg cgcggctccg tcgcttgttg gagcggccac
      781 tgtagcggac gccgccgcac ggtcggtcag cccggcggcg ccttcacgaa gtcggccagc
      841 gcctgcgcca gcgtctcgcg cacccgcggt gccggcagcg cggccatctg cggcagcgac
      901 atcgtcttgc ccagcccgcg cgcgatctgc agcccgaccg cgtagccggc tcgcggcggc
      961 cagccgtcgc cgtgctggcc gatgctgaag aaaagcgact gcgtcgcgtc atcccaggcg
     1021 tccagcgccg acagcatggc ccgcgcggcg gcgtgcagct gggagggcgt ggcctgcgcc
     1081 agctccgcgt tcaggagcac ggcctgcggc gaggcacccg ggttcatgca ttcgctggcc
     1141 caggtggccg tgccttccat ccagaggctc gcgtagaccg gcgctcgcgg gtccaggcac
     1201 atcgccgggt tcttctggcc ctggtagcaa tggaacagct cgtgcgagaa caggacggcc
     1261 agatcggcgt cggcaccgtg caggtagacg atcgcgtcgg gcccgaagaa cagcggcagg
     1321 cgcgggccgt cgggctgcag gtgcgcgtcg aagtgcatca gcgacggcat cagcgtgacc
     1381 ggcgacgcgc ggccatcgaa gtcggcgagc accgaacgga atcgcccgag gttgccggcg
     1441 tagccgtgcg cgaaacgccg gtggacgccg cgggctgcgg tggccaggcc gtcgaagcgc
     1501 ggcagccact tggcgacgtc ttccggcctg gccttgacac cggcctgcag gtacctgtcg
     1561 gcatgcttgc tgaagaagcc gtcgacgagc gcctgcgtgc gcgccggcgc cgcctcggcc
     1621 cgatgcgcgt cgtaggccat ccagaactcc ggcatcaggt cgaggaagcc gtcctgtcca
     1681 ggcggcgcct cgagggcagg tgcggcggca agcgccggca gggacagcgc cgctcccagc
     1741 agacccagca catcgcgccg cgtggcatcg aatcccatcg cccctcctct ccgttcctga
     1801 aagttgtcgc ccgtccggtc cgcacgcgac ggcggctggc gggtcacggt gtcagcgcag
     1861 caccttcaac gcatccggat tgacgatgtt ggtcggcgtg ccgttgatga agttgacgac
     1921 gttgtcgaac gcggcgccga agtacatctc gtagctgctc tgctcgacgt agccgatgtg
     1981 cggcgtgcag accgcattct ccaggcgcag cagcgggtgg ccggccagga tgggttcgct
     2041 ctcgaagacg tcgaccgcgg ccatgccggg gcggccgcgg ttgagcgccg ccacgagcgc
     2101 ggcctcctcg atgagctccg cgcgcgacgt gttgacgaac agcgccgtcg gcttcatctg
     2161 cagcaggtcg ttgagcttga tcaggtggcg cgtggactcg gtcaggcgca ggtgcacgct
     2221 gagcacgtcg gcgctggcga acagctcctc gcgcgaggca agcgcatggt ggccgtcggc
     2281 ctcggcgcgg gcgcgcgatt cctcggagcc ccacaccgcc acctccatgc cgaaagcctt
     2341 gccgtagccc gccaccagct tgccgatgcg gccgtagccc cagatgccca gcgtcttgct
     2401 gcgcagggtg gtgccgatgc cgaagttggg cggcatcgac gcggccttga gccccgactg
     2461 ctgccaggcg ccgtgcttga ggctggcgat gtagtgcggc agccggcgca tcgacgccat
     2521 gatcagcgcc caggtgagct cggccggcgc gtacggcgag ccgatgccct cggccaccgc
     2581 gatgccctgc tgcgtgcagg cctcgatgtc gatgtgctcg ccgaccttgc cggtctgcgc
     2641 gatcaggcgc agcttgggca gcttctcgag cagctggcgc gggaagcgcg tgcgctcgcg
     2701 gatcagcacc agcacctcgg cgtcgcgcag tcgcaccgac agctgcccga tccccttgac
     2761 ggtattggtg aacaccttgg cgttcagttc gctgagcttg gcggcgcacg gcagcttgcg
     2821 caccgcgtcc tggtagtcgt cgaggatgat gatgttcacg cggccgatta tggcccgcga
     2881 cggcaggccc gccgtgtcgg ccgaggacgc aaaaaagccg cctcgccggg cggctgttgc
     2941 atttgtcgca gcgcgcgtca gaagcgcgtc ggggcgggca ggaggccgct ggcggcgctg
     3001 ccgaaggttt ccgggaagat gcggtcgagc gagcggtaca gcgcggcgcg catcgcgcgc
     3061 gtggcgagct cgtcctcgcg cagcgccgac agcagcgggc cgcgcagcag ccacaggtcg
     3121 accggatcct cggcggcgcg cacctgcgcg cgcagccatg cgcccttgcg atgcgcgagg
     3181 ttctcgaccg cgctcatgaa cagctgcttc atctcgacga aggcgcgccg ggactcgatg
     3241 cggtcgccgc gcgaggcggg cagcgccacg gcgtccggga acgccgagtc ggacgcgaac
     3301 gccggctcgt agacgaagtc gatcgactcc ggcgccagtc gcgagtcgag ctcggacagg
     3361 cccagcgggt cgtaacggag gctagtggac atcggcaatc tccgcggccg gagcgctgct
     3421 gcgctgggca cgggtgggga agtgctggtt cacgcgggcc aggcgcacat cggcctcgac
     3481 ctggctgacg cggcgtgcga tcagcgtgta cagctccgag cgcaggtgcc acagctcgcg
     3541 cagcgagcgc gcgtgctggg cacgcttgag caggccgtgg ggatcgacct cctggaggcc
     3601 ggcgagcgcg ccgacgaact cggcgcgcgc gcgttccagc ggcgtgcgca gcgcgggcac
     3661 gacctcgacg tcgccaccca cgagccagcg cagcgtggcc gtccacaacg agtcgcgccg
     3721 gggcacgacg tccggcgggc agacattcat gcgcagcgtc gtcggcggcg cggccttgcg
     3781 atgcaggagg cgttggagga gggcggtgct catggttcga tttcggcccg cacgggctga
     3841 aacatgagca cattgtcaaa aactgctgcg agttgtaagg gccgaaaagg ccggtgcttc
     3901 ccgacagttc cgcgccgcgg gcgcggtcgg gcgggtcagc gtcccgcgct ggccgcgcct
     3961 tgcgggcgag gcgtgccgaa caccggctgc ggcaggccct ggcgcgcatg gtcgatcgcc
     4021 tcgcacagct cgcgcgcgcc gtcgacgaag cgcggtccgc tgcggtcgag caggtcgccc
     4081 tcgacgtggc cgaactgggc gtgctggacg gcgctgaccc gtttgaactg cgtccagcgg
     4141 ccgggcttgc ccggctcgcc cggcgcgccg gcggtcacca cgagctcggg gtcgcgctgc
     4201 acggcgacct cccagctcac ggtggcggtg agcgtgggca gttccgcgaa gacgttgctg
     4261 ccgccgcagg ccgcgatcgc gctggagacc aggtgctggc cgttgagcgt catcagcggc
     4321 gcgtcccaca gctggtagaa cacgcgcacc ggccggcggc cggcgtaggt cgccttcagt
     4381 gccgcccagt cggccgcgat ggcctgcgcc cgcgcctgcg cgacgccctc ggtgccggcc
     4441 agccggccga tacggcgcaa ggtatcgacc aggccggcga cggtgcggat ctcgatctcg
     4501 tagaccggca cgcccagcgt gcgcagcttg gccttgatgc gctcgggcgt gccgctgccc
     4561 cacaccagca cgaggtcggg cttgagtccg gccagcgcct cgtagttgac cgcgaacgcg
     4621 tcgctgatca cgggcttggc cagcgcgtcg ggcgggaagt cgctgtactt gccgaccgcc
     4681 accagcttgc cgccggcgcc ggccgcatag accagctcgg tcaggtgcgg cgcgagggcg
     4741 acgatgcgtt gcgccggatg ctgcagcgcg agctcgtggc cggcatcgtc gacgagcttg
     4801 agcgtcggcg tctgcgcgcg ggccgcggcc gcgagcagca gcgccgcggc cagggcgatg
     4861 gcgcgcgcct tcaaggctcg atccccgcgg cgcgcagcgc cttgccgtcg ggcgcggcct
     4921 ggcccggccg cggccgcgcc tgcggcggca gcgccttgaa ccagtcgcag gtttgcgtga
     4981 ccgcgcagcg tccgcactgc ggcatgcgcg ccaggcagac gtagcgaccg tgcaggatca
     5041 accagtggtg cgcgtcgtcg aggtatttct ccgggacgcg ctccagcagc ttgagctcga
     5101 ccgcgagcgg gttcttgccc ggcgccaggc cggtgcggtt ggcgacgcgg aacacgtgcg
     5161 tgtcgaccgc cagcgtcggc tcgccgaagg ccacgttgag gatgacgttg gccgtcttgc
     5221 ggcccacgcc cggcagcgcc tcgagcgcgg cgcggtcgcg cggcacctgg ccgccgtgct
     5281 gctcgatgag gatgcggcag gtctcgagca ggtgcttcgt cttgttgcgg tacaggccga
     5341 tcgtgcgcac gtgcgcctcg accccggccg tgccgagcgc cagcatgcgc gcgggggtgg
     5401 gcgcggccac gaacagcgtg cgcgtggcct tgttgacgcc cgcgtcggtg gcctgcgccg
     5461 acagcagcac cgcggtcagc agctcgaaca cgctcgcgta gtgcagctcg gacgccggcg
     5521 acgggttggc ggcctggagc gtggcgaaga aggtgtcgat gtctttttcg ttcattggaa
     5581 agcctgcgtc attcggactt cggtgcgagc cccttcgggc ggccgggcgg ggctcacaag
     5641 gcaacctccg ccctgcgggc tgcggtgcga gcgcccttcg gagcggccgg gcgggcgctc
     5701 atgcggcctt cgagcccgaa tcggcgaacc agcgccagac gatgggctgc aattgaacca
     5761 tcaggacgca ggccaggatc agcgcgcatc ccgccaggcc agcggcgccc atgcggtcgc
     5821 ccatgaagac ggcgccgaac gcggcggtga acaccgtctc cgacgacagg acgatggccg
     5881 agtccgcggg ccgcgtgtgc cgttgcgcga ccacctgcag cgtgaagccc agcccgaccg
     5941 acagcacgcc ggtgtacgcg atcggccaca gcgcttgccg gaagccgtcc gcggacagcg
     6001 gctcggtggc gaggccgaac gccagcgcga gcacgctgca ggcgatgaac tgcccgcagg
     6061 ccaccagcat cgcgccgtgg agcctgttgg cggcgcgtcc caccagcatc acgtgcaccg
     6121 accatggcag gctgctggcg atgatccacc agtcgcccgt gcggaacgcg gacaggctgc
     6181 cgccgccggt cagcagccac gagccgacga cgcaacccgc tgccgcgagc caggtcgtcc
     6241 agtgaggcgc ctggcgcgat gcgacccagg ccagcagcgg caccagcggg atgtacagcg
     6301 cggacaggaa gccggtgttg gtcacggtgg tggtggccat gcccatctgc tgcatggccg
     6361 cgccgaggaa cagcagcgtg cccaggcccg cgatgtgcgc gaggtcgcgt cggcgcggct
     6421 cgcggcgacg cgcgcgcagc atgtgccact cgcgccaggc cagcggggcg accaccgcgg
     6481 cgccgagcgc gaaccgcagc ccggtgaacg tcgacgcgcc caggtggcgc gcagccacgg
     6541 cctgcgccac gaaggccgag gcccagatca gcgagacccc cagcaggatc aggttcgagc
     6601 gcgcccgcgt cagcgggacg gcggtctgcg acggcaggac gacgtcgtcg aaggtcgacg
     6661 cttcggcctg gacgggctgc gtcattgtgc ccgcgccttc gcgcgcaggc gggcgcgggc
     6721 gagcgcggcc gcgacggcgg cgcgcttggg atcctcgggc gcggcctcga cgtcggcggg
     6781 cgcctcggcc ttcgcgctca ggcgcgcgtc gtgctcgcgc gcgtcacgcg cgaggcgcgc
     6841 ctgccggcgc gcgaagcggt cgcgcgcggt gtcggcccga gcggatgacc aggcgtccca
     6901 gccggtggcg ccggcactgg cctcctcgag cgcgatgcag tccatcgggc acacgggcag
     6961 gcacagctcg cagccggtgc agtcggcctc gacgaccgcg tgcatgcgct tgggtgcgcc
     7021 gatgatcgcg tcgaccgggc aggcctggat gcacagggtg cagccgatgc aggcggcttc
     7081 ctcgatgtag acgatgcggc gcggcgcctc gtctccgaac gcggggtcca gcggcagctc
     7141 gggctggccg agctcgcgcg cgaggcgggc gatgccttcg gcgccgccgg gcgggcactg
     7201 gttcagcctg gcctcgccag cggccagcgc ctcggcgtac gaacggcagt ccggatagcc
     7261 gcagcgcgtg cactgcgtct gcggcagcag ggcgtccagg cgctgcgcaa gcgatgcgac
     7321 gcccgcagga ggcgtcgcgg tctggaggtt gatggtgacg gtgggactca ccgtccggat
     7381 tatcccttcg cgggaggcgt gaccttgacg gtcctgcgcg ccttggtcgg ggatttggcg
     7441 gcggccgagg tggcgatcgc gcgcgacgtg cgcgccgccg tgggggcctt ggccgggcgg
     7501 ccggcgcgtt cctgctcgaa gcgcgcgatg aagtcgcgca cgtgcgggta gacgatgtcg
     7561 cgccagcggc ggcccgagaa gatgccgtag tggcccgcgc cctcgacgtc gtagtggaac
     7621 tggcgcgtct tggggatgcc ggtgcacagg ccgtgcgccg cgcgcgtctg gccggcgccc
     7681 gagatgtcgt cgagctcgcc ttcgatcgtc agcagggcgg acgaatggat gtcctgcggt
     7741 cgcaccaggt ggccgtcgac gtcccacgtg cccttgacca gcgagaagtc ctggaacacg
     7801 gtcttgatgg tgtccaggta gtactcggcg ggcaggtcga gcacggcgtt gtactcgtcg
     7861 tagaacttgc ggtgcgcctc ggtggagtcg tcgtcgccgc gcaccaggtc gaggaagtag
     7921 tcccagtggg ccgtcgcatg gcggtccgga ttcatcgcga cgaagccggt gtgctgcagg
     7981 aagcccgggt agacctggcg ctgcgcgccc ggatagttgg gcggcacgcg gtggatcacg
     8041 ttgttctcga accactcgta gctgcggttc atcgcgaggt tgttgaccgc cgtgggcgaa
     8101 ttgcgggcgt cgatcgggcc acccatcatc gtcaggctca gcggcgtggt ctcgccggcg
     8161 ctggccatca gcgagaccgc ggcgagcacg ggcacggtgg gctggcacac ggagatcacg
     8221 tgcacgtccg cgccgatcag gcggatgaac tcctgcacgt agcggatgta gtcgtccagg
     8281 tggaacgggc cggcctcgac cggcaccatg cgggcatcgg tccagtcggt gatccagacc
     8341 ttgtggtcct gcagcaggct gcgcacggtg tcgcgcagca gggtcgagtg gtggccggac
     8401 agcggcgcca cgaccagcac cgagggctgc gccttcatgc gatcgagcgt ggccgagtcg
     8461 tcgctgtagc gcttgaagcg gatcaggcgg cagaagggct tttcgagcgt gacctgctcc
     8521 tgcacggcca cgtcgacgcc gcccgacttg acgttcttga tctcgaactc gggcttctcg
     8581 tagtccatgc ccagccggtg catcagctcg aagccggccg acaggcgctg ggcgcccggg
     8641 atgtgcgtga acggcgacag cggatgcttg tagagcttgg cggtggcggc cgcgaaatcg
     8701 gcgaagggcg ccatcagggc gcgctgcgct tcgtagagtt ggtaaagcat atcgctctca
     8761 gcctttcggg ctcgttacct ggcaacgcga cagtggccgc gcgtcgggga ttctcttcga
     8821 ttcctgaacg aaagcttgtc accttcgttc gtttattaat tgctgcgccg cagcattggt
     8881 tcaatagtac gcctatttgc ggtggtccgt gcaactccgc aacgtgcatg cctggcgtcc
     8941 cgtcggggac tcccgggcct gtcacgatga tgcaacctgc tgcgatgcac caatcggccg
     9001 aacaggggcc ggaagcgcgc ccatccgtga cggtgttggc accctttgtc gagttgctcc
     9061 ggcccgccac gccgttccgt tgcgcaacag gtggggccgg ccgtgcaacg aaaaaggccc
     9121 cggagcgctc gccccggggc ctttccagtg gagtggaccg tcgatcaggc cgacacggcc
     9181 gcgatcgcct tggccacgta ggccaggttg tggtcgttca gggcggccac gcagatgcgg
     9241 cccgagtcga cgccgtagac gccgaactcg ctgcgcaggc gctgcatctg ggcggccgac
     9301 aggcccgagt agctgaacat gccgttctgc ctggtgatgt agctcaggtc gcccttgacg
     9361 ccggcgttgg tcaactggtc gaccagcgcg ccgcgcatgc gcttgatgcg gtcgcgcatg
     9421 cccgccagct cggactccca ctgcgcgcgc agcgccggcg tggtcagcac ggtggccacg
     9481 atctgcgcgc cgtgcgtcgg cgggttggag tagttggtgc ggatgacgat cttgagctgc
     9541 gacagcacgc gcgcggcttc ctccttgctc gagcacacga cgctcagcgc gcccacgcgc
     9601 tcgccgtaca gcgagaagct cttggagaac gaggtcgaaa cgaagaagtc caggcccgag
     9661 gccatgaact ggcgcacggc ggcgccgtcc tcggcgatgc cgaagccgaa gccctggtag
     9721 gccatgtcga cgaagggcac caggccgtgc tccctgatcg tggcgatgac cttcgtccac
     9781 tgctcggccg acaggtcgac gcccgtcggg ttgtggcagc aggcgtgcag cacgacgatg
     9841 gtgccggcgg gcgacgacgc cagcttggcg atcatcgcgt cgaaggcgac gccgcgcgtg
     9901 gccgcgtcgt agtacgggta ctgctcgacg gcgaagccgg cgttggtgaa cagcgcgcgg
     9961 tggttctccc agctcggatc gctgatcagc accttcgcgt ccggacgcag gcgcttgagg
    10021 aaatcggcgc cgaccttcag gccgccggtg ccgccgagcg cctgga
//
LOCUS       DATCRO010000131         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_170070, whole genome shotgun sequence.
ACCESSION   DATCRO010000131 DATCRO010000000
VERSION     DATCRO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170070"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VIN75_03585"
     CDS             complement(<1..1010)
                     /locus_tag="VIN75_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY8973274.1"
                     /translation="MTATQAAAARRPVVWTISGTDSGGGAGIHADTRALDAFGVHGAC
                     AVAAVTAQHSTAVTHIEPVRPEVLDAQLAALADDMPPDAIKTGLLGSLANLRVVAGWV
                     RRVRARFPERAIPLVVDPVFGATTGAAFVDDALRTAYRDELLPLATLATPNVAEARAL
                     VPSSQLLGPEALARAWRAFGVRAVAVTGGDSDRLADDEIEHLSTARLASRLAEIDARA
                     AWAHDWLDAEHGLRCRGWLASRRIETEHDHGTGCVFASSAAAALAHGFVAADAVVLAK
                     MATSEGLRHAYAAGGGAGPVAPRAGFAMRLANLPALNPLPHAPSRFAFPEMPRHARGL
                     YA"
     gene            complement(1012..1836)
                     /locus_tag="VIN75_03590"
     CDS             complement(1012..1836)
                     /locus_tag="VIN75_03590"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY8973275.1"
                     /translation="MRLDFSAAAPSPWRAYGQRLDSRFLIGSAGYPSPEVLAHAITAS
                     GAQVVTVGLKREIAGAAPQGSEAVAMLRGVCAASGARLLPNTAGCRSAREAVLLAQMS
                     RELFDTPWIKLEVIGDEHTLQPDPFELVEATAQLVKLGFQVFPYCTDDLVVCRRLLAA
                     GAEVLMPWAAPIGSGQGVLDPAALRTLRARLPGVPLIVDAGLGAPSHAAFAMELGYDA
                     VLLNSAVAQAVNPPQMAMAFAHAIAAGRLGHEAGLIAPQDMAIPSTPCGNEPILVD"
     gene            complement(1839..2084)
                     /gene="thiS"
                     /locus_tag="VIN75_03595"
     CDS             complement(1839..2084)
                     /gene="thiS"
                     /locus_tag="VIN75_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY8973276.1"
                     /translation="MREGDFVFDPPPVRTATVLVNGQPHPWHLGLTVADVLAQRGSDD
                     ENVATAVNTRFVPRPQRHETPLWPDDRVVVFAAIVGG"
     gene            complement(2077..3252)
                     /locus_tag="VIN75_03600"
     CDS             complement(2077..3252)
                     /locus_tag="VIN75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973277.1"
                     /translation="MSRIGIAGAGIAGRLLAFQLSRLGHEVDVFDPAPDAGAPLGQQA
                     HPAAAFTAAGMLSPLAELEHAPADISERGWRSLDLWPGIVAALGPAYGTPGLFRRNGS
                     VLVAHPGDTGATQRVLARLKAANARSARQRDADAADATHPTTLQLNGPRDKPALRDPQ
                     ALTPEMLRELEPALIPGVQGWLLPGEGQVDPSRLLPALVAESPQVRWHWNHRVAHVAP
                     HLVQVLGRTTDKFDFAIDTRGLGARGQGLALRGVRGELLWLHAPGLELRRPVRCIHAR
                     HRVYLVPRQGDHVIVGATEIDSEDRSPVSVRSAIELLSAAHSIVPQLAEARIVRLDTH
                     LRPATLDQRPHLHCKPGLIRINGLFRHGFLLAPALVEEALERTGLMLGARRRELSHA"
     gene            complement(3449..4202)
                     /locus_tag="VIN75_03605"
                     /pseudo
     CDS             complement(3449..4202)
                     /locus_tag="VIN75_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
BASE COUNT          544 a         1530 c         1586 g          542 t
ORIGIN      
        1 atggcgtaca ggccgcgcgc gtgccggggc atctcgggaa acgcgaagcg cgacggcgcg
       61 tgcggcaacg ggttgagcgc cggcaggttg gcgagccgca tcgcgaagcc cgcgcgcggc
      121 gccaccggcc cggcgccgcc gccggccgcg tacgcgtggc gcaggccttc gctggtggcc
      181 atcttcgcca gcaccacggc gtcggcagcg acgaagccgt gcgccagcgc ggccgcggcc
      241 gacgatgcga acacgcagcc ggtgccgtgg tcgtgctccg tctcgatgcg gcgcgacgcc
      301 agccagccgc ggcagcgcag gccgtgttcc gcgtcgagcc agtcgtgcgc ccacgcggcg
      361 cgggcgtcga tctcggccag ccgcgacgcc agcctcgcgg tggacaggtg ctcgatctcg
      421 tcgtcggcca gccggtcgga gtcgccgccc gtgacggcca ccgcgcgcac gccgaacgcg
      481 cgccaggcgc gggcgagcgc ctcggggccc agcaactgcg acgaaggcac cagcgcgcgc
      541 gcctcggcca cgttgggcgt ggccagcgtg gccagcggca gcagttcgtc gcggtaggcg
      601 gtgcgcagcg cgtcgtcgac gaaggcggca ccggtcgtcg cgccgaacac cgggtcgacg
      661 accagcggga tcgcgcgttc gggaaagcgc gcgcgcacgc gccgcaccca gcccgcgacg
      721 acgcgcaggt tggccaggct gcccagcagg cccgtcttga tcgcgtcggg cggcatgtcg
      781 tcggccagcg cggccagctg cgcgtccagc acctcgggtc ggaccggctc gatgtgcgtg
      841 acggccgtcg agtgctgcgc cgtcaccgcc gccaccgcgc atgcgccgtg cacgccgaag
      901 gcgtcgagcg cgcgcgtgtc ggcgtggatg ccggcgccgc cgccggagtc ggtgccggag
      961 atggtccaca ccacggggcg gcgcgcggcc gctgcttgcg tcgccgtcat ctcaatcgac
     1021 caggatgggt tcgttgccgc agggcgtgct ggggatggcc atgtcctgcg gcgcgatcag
     1081 cccggcctcg tggcccaggc ggcccgcggc gatcgcgtgc gcgaacgcca tcgccatctg
     1141 cggcgggttc acggcctggg cgacggccga gttcagcagc accgcgtcgt agcccagctc
     1201 catcgcgaac gccgcgtgcg acggcgcccc gaggccggcg tccacgatca gcggcacgcc
     1261 cggcagccgc gcgcgcagcg tgcgcagcgc ggcggggtcg agcacgccct ggcccgaacc
     1321 gatcggtgcg gcccacggca tcagcacctc ggcgcccgcg gccagcagcc ggcggcagac
     1381 gacgaggtcg tcggtgcagt acgggaacac ctggaagccc agcttgacca gctgcgcggt
     1441 ggcctcgacc agctcgaacg gatcgggctg cagcgtgtgc tcgtcgccga tcacctccag
     1501 cttgatccac ggcgtgtcga acagctcgcg cgacatctgc gccagcagca ccgcctcgcg
     1561 ggcgctgcgg cagccggccg tgttgggcag cagccgcgcg ccgctggcgg cgcagacgcc
     1621 gcgcagcatg gccacggcct cgctgccctg cggcgcggcg ccggcgatct cgcgcttcag
     1681 gcccacggtg accacctgcg cgcccgaggc cgtgatggcg tgcgccagca cttccggcga
     1741 cgggtagccg gcgctgccga tcaggaagcg gctgtccagg cgctggccgt aggcgcgcca
     1801 cggcgagggc gccgcggcgg agaaatcgag ccgcatcgtc agcctcccac gatggccgcg
     1861 aacacgacga cgcggtcgtc gggccacagc ggcgtctcgt ggcgctgcgg gcgcggcacg
     1921 aagcgcgtgt tgacggcggt ggcgacgttc tcgtcgtcgc tgccgcgctg cgcgagcacg
     1981 tcggccacgg tcaggcccag gtgccacgga tggggctggc cgttgaccag cacggtggcg
     2041 gtcctgaccg gcggcggatc gaacacgaag tcgccttcac gcatggctca gctcccggcg
     2101 gcgcgcgccc agcatcaggc ccgtgcgctc cagcgcctcc tcgaccagcg cgggcgccag
     2161 caggaagccg tggcggaaca gtccgttgat gcggatcagg cccggcttgc agtgcaggtg
     2221 cggccgctgg tcgagcgtgg ccggacgcag gtgcgtgtcc agccgcacga tgcgcgcctc
     2281 ggccagctgc ggcacgatgc tgtgcgcggc cgacagcagc tcgatcgcgc tgcgcacgct
     2341 caccggcgag cggtcctcgc tgtcgatctc ggtggcgccg acgatcacgt ggtcgccctg
     2401 gcgcggcacc agatagacgc gatggcgcgc atggatgcag cgcaccggcc ggcgcagctc
     2461 gagacccggc gcatgcagcc acagcagctc gccgcgcacg ccgcgcagcg ccaggccctg
     2521 tccgcgagcg cccaggccgc gcgtgtcgat cgcgaagtcg aacttgtccg tcgtgcgccc
     2581 gagcacctgc accaggtgcg gcgcgacgtg cgccacgcga tggttccagt gccagcgcac
     2641 ctgcgggctc tcggcgacca gcgcgggcag cagccgcgac gggtcgacct ggccttcgcc
     2701 gggcaggagc cagccctgca cgccgggaat caatgcgggc tcgagctcgc gcagcatctc
     2761 cggcgtcagg gcctgcgggt cgcgcagcgc cggcttgtcg cgcgggccgt tcagctgcag
     2821 cgtggtcgga tgcgtcgcgt cggcggcgtc ggcgtcgcgc tggcgcgcgc tgcgcgcgtt
     2881 ggccgccttc agccgcgcga gcacgcgctg cgtcgcgccg gtatcgccgg gatgcgccac
     2941 cagcacgctg ccgttgcgcc ggaacaggcc cggcgtgccg tacgccggcc ccaacgcggc
     3001 gacgatgccg ggccacaggt cgagcgagcg ccagccgcgc tcggagatgt cggccggcgc
     3061 gtgctcgagc tcggccagcg ggctgagcat cccggccgcg gtgaaggccg cggcgggatg
     3121 ggcctgctgg cccagcggcg cgccggcgtc gggcgcggga tcgaacacgt cgacctcgtg
     3181 gccgagacgc gaaagctgga acgcgagcaa ccgtccggcg atgcccgcgc ccgcgatgcc
     3241 gatgcggctc atgggtgcac ccctggcgct gcgcgcctcc ccgccgagcg cgggcattcg
     3301 cccttcgggc gaccgctgac gttcattgat cctccgagtc gtagatgggc acgaagggct
     3361 tgcccagcgt cgtgcgccgc gtcaggcccg tggcggagac gcgggccacc gagccggccg
     3421 catccagcgt cgcgtgggcc tgcgcggcgg cgaactcgcg cacctcgtgc gtggtcttca
     3481 tcgagcagaa cttcgggccg cacatgctgc agaagtgcgc ctccttggcg ccctcggcgg
     3541 gcagcgtcgc gtcgtggtac tcgcgcgccg tgtcggggtc gagcgccagg ttgaactggt
     3601 cgtgccagcg gaactcgaag cgcgccttgg acatggcgtc gtcgcgcgcg cgggcgcccg
     3661 ggtggccctt ggcgacgtcg gccgcgtggg ccgcgatcct gtaggcgatc aggccctgct
     3721 tgacgtcgtc gcggtcgggc aggcccaggt gctccttggg cgtcacgtag cacagcatcg
     3781 cggtgcccat ccagccgatc atcgcggcgc cgatcgagct ggcgatgtgg tcgtagccgg
     3841 gcgagatgtc gatggtcagc gggcccagcg tgtagaacgg cgcgtcgtgg cacaggcgca
     3901 gctgctcggt catgttggcc tggatcaggt gcatcggcac gtggccgggg ccctcgatca
     3961 tcgtctgcac gtcgtgcttc caggccacct gcgtgagctc gccgagcgtg cgcagctcgg
     4021 acatctgcgc gtcgtcgttg gcgtcggcca gcgagccggg ccgcaggccg tcgcccagcg
     4081 agaacgagac gtcgtacgcc ttcatgatct cgcagatctc ctcgaagtgc gtgaacagga
     4141 agttctggca gccgtgcgcc atgcaccact tggccaggat cgcgccgccg cgcgacacga
     4201 tg
//
LOCUS       DATCRO010000132        11686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_260046, whole genome shotgun sequence.
ACCESSION   DATCRO010000132 DATCRO010000000
VERSION     DATCRO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260046"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VIN75_03610"
     CDS             complement(<1..350)
                     /locus_tag="VIN75_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973278.1"
                     /translation="MSCDNDCTRPDRFPKTIENRPGLTTLDTRIGSYADLRRHMLRRV
                     DEHPALAAWTHRLPDDPGIALIEAAAEVGDILSFYQDLYANEAYLGTAKWRDSVGLLV
                     RLLGYRLAPGTGGRA"
     gene            complement(347..2914)
                     /locus_tag="VIN75_03615"
     CDS             complement(347..2914)
                     /locus_tag="VIN75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY8973279.1"
                     /translation="MTMLRDAEALEDRARDLQGRGLNAFRLGVLTLDAAPKPAFAWLE
                     LQFWSDVALAPLPPPAAFTVGGAARQRGGNPARPVRVVEVQAGGAADTLRLKIEPVGD
                     YSTYLLSTTPGGLSAPDDALPRAMDPLLNALPFKFRPGCFNLGCTPAPRYEPAPVEPS
                     IDYLARDYDSFRHVLIAAMMRRVPGWRVTSEADLDQVLIDLLAADADEQADYHDRVMA
                     ERSIATARKRVSLARHARLMDYHVHQGNQAGTWLALEVAAQAELPAAATDEFGVWTGR
                     SWDDDGAVIFAMPREGDRWRRRAFPALNRLRLYTWGGAVRALAAGSTEADLTGLAPLD
                     QAGAEALRDLFLGQAPGQAGVADDVDAGVDRLLVQEWLNPDTGTINGARLDQRQVVRL
                     LPPGGPVPRAEALPDPVTGEWFVRVRWRDEDALARPFCFLCDCNDVTVVDVSLFHANL
                     VRVTQGRPRVTVFRAPGQPVAPLDDSALVARDGATYERVVRQRGASLVPRGVLCHLPA
                     APLAYVDTAPGGEAATRTTALVQVDGARWREQSDLIASEGDDEHFLVETDELGGSSLR
                     LGDGINGAALADGAIVTCRYRVGQGTAGNVGADRLVGFIDPSGAVQRVWNPLDVTNGR
                     DPEPAAEIVRRVPEAYRQRQLRAVTLADYARRAEELPGVAHARARYAWTGSWRTVRVA
                     IDPVGTVVLDEPLRARIADHLEAVRLIGEDLEVRPATYVPLDITMTLCAKAAYWPEDL
                     RAELEEAFSDGWTRDGRRGFFHPDAWTFGQPLHASQLIGRALAVTGVGRVTRLSLRRW
                     HTGGGGGLTVIDIDPSALPQGHVDKLAIGPFEILVVANDPDHLETGRIRFEITGGRR"
     gene            complement(2914..3354)
                     /locus_tag="VIN75_03620"
     CDS             complement(2914..3354)
                     /locus_tag="VIN75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY8973280.1"
                     /translation="MNPSDASTSTTMPERAWPEVARPDGRHLAFPFRIGPDGRTATPA
                     DLSDHVRGEIIQLLLTAPGERPFLPGFGAGLRRLVFQRNDDATAGITKALVSRNLSRW
                     LGHRVSVQALDVSAEDATLRVDLRYEVVATGEVKQVRFERQGAL"
     gene            complement(3357..3650)
                     /locus_tag="VIN75_03625"
     CDS             complement(3357..3650)
                     /locus_tag="VIN75_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973281.1"
                     /translation="MADPLKAGQAGDFAGSLADYIDRAMQAEWLAVKHEALPSGADDI
                     GVEDRRILFAAVAQGVLAFLRDNRQNLVTTDENGGGLDPHHHAMNFTVSSTRV"
     gene            complement(3660..4379)
                     /locus_tag="VIN75_03630"
     CDS             complement(3660..4379)
                     /locus_tag="VIN75_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY8973282.1"
                     /translation="MDTGTLEQLVVRLAQQAGQRHWGKYRGIVTDNQDPSAIGRVKAQ
                     VPEVLGADVVTPWATPCAPYAGPGVGLHAVPPVGAGVWIEFEAGDPSRPIWSGGWWGT
                     ADLPKDEQGTAAKPPLKILRSEQGLMVSLDDDAHTLTLSDANGSNLVTIRVDDGQIKV
                     QAATKVIVEAPKIELVDGATHPLVFGDNLLQYLNQLVSLFNTHLHVGQTAIGIPVTPM
                     QPVVPFPPAPPSLLSQRVTTG"
     gene            complement(4379..5494)
                     /locus_tag="VIN75_03635"
     CDS             complement(4379..5494)
                     /locus_tag="VIN75_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973283.1"
                     /translation="MARAEYRVYFDNAPASQEQLERFTEVRVDQGIGIAAEAELEWPV
                     STDTQGRWLAAEESYARVRIEVKVADGDFVPLIDGPVVGRRYEMSAIPDQSRMTLVVH
                     DDSALLDRDEKVALFEDMAPQDIVSSVFAEAGLTPDVDAVSAAGGALQRAVVQRGTGM
                     QLVRELARSHGMFAFVRPGDAPGLSVGAFKRLAPAPGTLPELLLVGDQRNVARFSAEY
                     DALRPRQAQAGSVALADLTVLSSTATDASQAPLGDVAAHEDAVSGMTPGIALLTGTRE
                     EQTDLDAATAAAVDVSSWAWSASGELDADRYTGVLRPYEVIRVRGVGGYLSGDYLIGR
                     VTHVLNDDAYRQRFSLHRNARSAGSGGAAGGLPAGVF"
     gene            complement(5498..5770)
                     /locus_tag="VIN75_03640"
     CDS             complement(5498..5770)
                     /locus_tag="VIN75_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973284.1"
                     /translation="MFLKNSRYAGLPLVTAKNRAGEDVAAVVLRPLPSSGGDAVTVRA
                     HDQLDAMAEQRYGDVTRYWHVADANAELEAASLLRPAGRPIDVPKG"
     gene            complement(5793..6482)
                     /locus_tag="VIN75_03645"
     CDS             complement(5793..6482)
                     /locus_tag="VIN75_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973285.1"
                     /translation="MALLPLRGVLVEYGINLLGPIPNIVVFQFNPDQMTRTIAVPQPP
                     QSASAQAQAERDRASAPPTESFEITAQFSAADDLGAGGAVSAIPRVFGVGPQLAALEK
                     MVFPPGTPGGLIGAAIDAIASAISGPGAAAPTQPIPRLALPRLLFIWGPSRVLPVTIT
                     SMVITEQKYDVLLNPIQAEVRIGLQVSSVAPTDDAVGQGAYEYSRMVKDTQATLNLAK
                     VAEFALDIIPF"
     gene            complement(6484..6825)
                     /locus_tag="VIN75_03650"
     CDS             complement(6484..6825)
                     /locus_tag="VIN75_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973286.1"
                     /translation="MNVEIGRLDVSLRTADRHGADALGADVAAQVQAALRDALTRLDG
                     IAARGGATRLDLGRIDAPAGADARAVAGLIAARLALWLSRDPGDTPSSMATSTSTPPS
                     TSSSASPLEER"
     gene            complement(6822..9074)
                     /locus_tag="VIN75_03655"
     CDS             complement(6822..9074)
                     /locus_tag="VIN75_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973287.1"
                     /translation="MNAPHALPIPSTLVEPVALNPDLAVADAACAWWLAQVTLRLRRE
                     VAWCWHQRGLWPRAPGELPAIGDASQDSLDLLRFRDDKRRFFETDVTARYLDERLAAF
                     DLPERALDRAPERRGDWRWIARECQLTPAAQFVLACALMGRADAAIGPVCAACMNDGT
                     RPWPTLALAQRLWDEPVEIAACADAGHALFRHGLLNLPPGRDALDWTQPLEMPPRVAH
                     ELLRVVEEGTDTHGRVETRARTLAEGAAQATVVRPIDDTDAQRARLARLRSDPPVAMQ
                     VLPLLGPRGTDFAAAARAIARACGRGLLVVDGDVTPDRGLAPLATVAWLRGADLLLPE
                     QWQERCCAAAGEGWTTPLAALPLRWMLPMQDGTAAGAAGTGSAASTGGPSGRGVPAAL
                     LLPAWPMTPPDTAERTARFDAALADAPPALRAAGADAARRFRLTDRPLARVAAAARGL
                     PPDAGASALFALCRAEARLELDGLAQAVEPRFALEELVLPPSQARQLREIVLAMRALA
                     RVHHDWGTARAWNEGGLSVLFCGPPGTGKTMAAEALASELSLPMYRIDLSQVVNKYIG
                     ETEKNLRRIFDAAETGDCLMFFDEADALFGKRTEVRDAHDRFANIEVSYLLERMERFK
                     GLAVLASNRRKDLDDAFTRRLRHIVEFPMPGAAERERLWRQAFPARVDARSLDVSWLA
                     QQFELAGGSIRSAAFNACLLAAGRDPAAPPRVDMPAVLAAVRRELEKMNRVAGCQQFG
                     RYAPLLEESP"
     gene            complement(9079..10236)
                     /locus_tag="VIN75_03660"
     CDS             complement(9079..10236)
                     /locus_tag="VIN75_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY8973288.1"
                     /translation="MSLADSRDAIGAVSDLLKTALATASGISAITVGRPEEAAKAAQS
                     GQGSLNLFLYRVAVDGDLRNRALDAGQAPPVWLVLHYLLTAFDGPDSDSIAAHRLLGR
                     GIRGLNALSVLKPGAAQLPLASSPDPLRITLDEADVEMLSRVMQGSDEKYRVSAGFQV
                     RPVMVASTDEPPAYAPPVKTIGQPAPAPRMYDGVTVLPSLGAVATAIEPERFEAGQAF
                     VVRGTDFAGYDQIQIGPHVYPAVATPDGGLTATIAAADAVEPGAYPVCVARALPGGRL
                     TSSNAVTGRLSPRITAVNLAGVLTVVGTRRSGSFVVLGSQLSGPNSAAYAALYLDGQS
                     RLLLEPQPGGTPNSLTFVVDDDHALPPSTEYRVILRVNGQQAFDTPQLNWT"
     gene            complement(10240..11280)
                     /locus_tag="VIN75_03665"
     CDS             complement(10240..11280)
                     /locus_tag="VIN75_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973289.1"
                     /translation="MSGYFARLAAQAAPRTARSSSARSPHSSSFMAMAAPREAASLQV
                     STARDNASDQTSRMSPGVVPSSALDVSSNARGTLRSRSSDASFPMDTQRGPHAARALA
                     VDEDIARSATHTATRSVASSIDAFAGTSPEPSTNGLTAASRDASTAASTARDARPGIV
                     EPGRMPGMAAEPVDRRGGRDDVDMAHAPQVPTTTASSTSFETPAPAIAAASTRSDESS
                     AAASQDVIEAAEAFSVQPQPQVRAEGRATESEAWEDGRAGAGTSAETRRAVAARNEAA
                     IDTLTTGAASRMTGGAAAPRIRIGQIALEVHVAPPPSRPAAPPVPQPGPIPAAAPAPP
                     RFSARRHYLRWG"
     gene            complement(11277..>11686)
                     /locus_tag="VIN75_03670"
     CDS             complement(11277..>11686)
                     /locus_tag="VIN75_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973290.1"
                     /translation="RLPCGDDLSSWPGRVASPEQIAVTLIESVDGRPLADGEPCPAPL
                     LAALDTIYEAHDPLTALKLHAACPHCGRPNDVACDLEAALLAGFARQQRGLMDEVVRL
                     ASAFHWSEADILSLPAWRRAHYLRRLDQVEGWA"
BASE COUNT         1523 a         4314 c         4320 g         1529 t
ORIGIN      
        1 gcgcggccgc cggtgccggg ggcgagccgg tagcccagca gccgcaccag caggcccacg
       61 ctgtcacgcc atttcgcggt gccgaggtag gcctcgttgg cgtagaggtc ctggtagaag
      121 ctgaggatgt cgccgacctc ggcggccgcc tcgatcagcg cgatgccggg atcgtcgggc
      181 agccggtgcg tccacgcggc cagcgccgga tgttcgtcga cgcggcgcag catgtggcgg
      241 cgcaggtcgg catagctgcc gatgcgggtg tccagcgtcg tcaggccggg tcggttctcg
      301 atcgtcttcg ggaagcggtc cggccgcgtg cagtcgttgt cgcagctcat cgacggcctc
      361 cggtgatctc gaagcggatg cggccggtct ccaggtgatc cgggtcgttc gcgacgacga
      421 ggatctcgaa cggcccgatc gccagcttgt cgacatgacc ctgcggcagc gccgacggat
      481 cgatgtcgat gaccgtcagc ccgccgccgc cgccggtgtg ccagcgccgc aggctgagcc
      541 gcgtcacccg accgacgccg gtcacggcga gcgcgcggcc gatcagctgg ctcgcatgca
      601 gcggctggcc gaaggtccag gcatccggat ggaagaagcc gcgccggccg tcgcgggtcc
      661 agccgtcgga gaaggcctcc tccagctcgg cgcgcaggtc ctcgggccag taggcggcct
      721 tcgcgcacag cgtcatcgtg atgtccagcg gcacgtacgt ggccggccgc acctcgaggt
      781 cctcgccgat caggcgcacc gcttcgaggt ggtcggcgat ccgggcgcgc agcggctcgt
      841 ccagcaccac cgtccccacc ggatcgatgg ccacgcgcac cgtgcgccag ctgccggtcc
      901 aggcgtagcg cgcgcgggca tgggcgacgc cgggcagctc ctcggcgcgg cgcgcgtagt
      961 cggccagcgt gaccgcgcgc agctggcgct ggcggtaggc ctcgggcacg cggcgcacga
     1021 tctcggccgc cggctccgga tcgcggccgt tggtgacgtc cagcggattc cagacccgct
     1081 gcaccgcgcc gctcggatcg atgaagccga ccagccggtc cgcgccgacg ttgcccgccg
     1141 tgccctgtcc cacgcggtag cggcaggtga cgatcgcgcc gtcggccagc gccgcgccgt
     1201 tgatgccgtc gcccaggcgc agcgagctgc cgccgagctc gtcggtctcg accaggaaat
     1261 gctcgtcgtc gccctcgctg gcgatcaggt cggactgctc gcgccagcgc gcgccatcga
     1321 cctgcaccag cgcggtcgtg cgcgtcgccg cctcgccgcc gggtgcggtg tccacatacg
     1381 ccagcggtgc cgccggcagg tggcacagca cgccgcgcgg caccagcgac gcgccgcgct
     1441 ggcgcaccac ccgctcgtag gtcgcgccgt cgcgcgcgac cagcgcgctg tcgtcgagcg
     1501 gcgcgacggg ctggccgggc gcgcggaaca cggtcacccg cggccggccc tgcgtcaccc
     1561 ggaccaggtt ggcgtggaac aggctgacgt cgaccaccgt cacgtcgttg cagtcgcaga
     1621 ggaagcagaa cggccgcgcc agcgcgtcct cgtcgcgcca gcgcacccgc acgaaccatt
     1681 cgccggtgac agggtcgggc agcgcctcgg cgcgcggcac cgggccgccc ggcggcagca
     1741 ggcgcacgac ctggcgctgg tccagccgcg cgccgttgat cgtgccggtg tcgggattga
     1801 gccattcctg gaccagcagc cggtccacgc cggcatcgac gtcgtcggcc acgcccgcct
     1861 gccccggcgc ctggccgagg aacaggtcgc gcagcgcctc ggcgcccgcc tggtccaacg
     1921 gcgccaggcc ggtgaggtcg gcctcggtgc tgcccgcggc cagcgcgcgc accgcgccgc
     1981 cccaggtgta cagccgcagc cggttgagcg ccgggaaggc gcgccgccgc cagcggtcgc
     2041 cctcgcgcgg catcgcgaag atcaccgcgc cgtcgtcgtc ccaactccgg ccggtccaga
     2101 cgccgaattc atcggtcgcg gcggccggca gctcggcctg cgccgcgacc tccagcgcga
     2161 gccaggtccc ggcctggttg ccctggtgga cgtggtaatc catcagccgc gcatgccgtg
     2221 ccagcgagac ccgcttgcgc gcggtcgcga tcgagcgctc cgccatcacg cggtcgtggt
     2281 aatcggcctg ctcgtcggcg tcggcggcga gcaggtcgat cagcacctga tccaggtcag
     2341 cctcgctggt gaccctccag ccggggacgc gccgcatcat cgcggcgatc agcacatggc
     2401 ggaaggagtc gtagtctcgg gcgaggtagt cgatcgacgg ctcgaccggc gccggctcgt
     2461 agcgcggtgc cggcgtgcag ccgaggttga agcagcccgg gcggaacttg aacggcagcg
     2521 cgttgagcag cggatccatc gcccgcggca gcgcgtcgtc gggcgccgac aggccgccgg
     2581 gcgtcgtcga cagcaggtag gtcgagtagt cgccgaccgg ctcgatcttc aggcgcagcg
     2641 tgtcggccgc gccgccggcc tgcacctcga cgacgcgcac cggccgcgcc ggattgccgc
     2701 cgcgctggcg cgccgcgccg ccgaccgtga acgccgccgg cggcggcagc ggcgccagcg
     2761 cgacgtcgct ccagaactgc agctccagcc aggcgaaggc cggcttgggc gccgcgtcca
     2821 gcgtgagcac gccgagccgg aacgcgttca gcccccggcc ctggaggtca cgggcgcggt
     2881 cctcgagcgc ttcggcatcg cgcagcatcg tcatcacagc gctccctggc gctcgaagcg
     2941 cacctgcttg acctcgccgg tggcgaccac ctcgtagcgc aggtccacgc gcagcgtcgc
     3001 gtcctcggcg ctgacgtcca gcgcctgcac gctgacgcgg tggcccagcc agcgcgacag
     3061 gttgcggctg accagcgcct tggtgatgcc ggcggtggcg tcgtcgttgc gctggaagac
     3121 cagccggcgc aggcccgcgc cgaagcccgg caggaagggc cgctcgccgg gcgcggtcag
     3181 cagcagctgg atgatctcgc cgcgcacatg gtccgaaagg tcggccggcg tggccgtgcg
     3241 gccgtccggc ccgatgcgga acgggaaggc caggtgccgg ccgtcgggcc gggcgacctc
     3301 cggccaggcg cgctcgggca tcgtggtcga ggtcgaggca tcggacgggt tcatggtcac
     3361 accctcgtcg agctgacggt gaagttcatc gcgtggtgat gcgggtccag cccgccgccg
     3421 ttctcgtcgg tcgtcaccag gttctggcgg ttgtcgcgca ggaaggccag cacgccctgc
     3481 gcgaccgcgg cgaacaggat gcggcggtcc tccacgccga tgtcgtcggc gccgctgggc
     3541 agggcctcgt gcttgacggc cagccactcg gcctgcatcg cgcgatcgat gtagtcggcg
     3601 aggctgccgg cgaagtcgcc ggcttgaccg gccttgagcg gatcggccat gaatgctcct
     3661 caaccggtgg tgacgcgctg cgacagcagc gacggcgggg ccggcggaaa cgggaccacc
     3721 ggctgcatcg gcgtcaccgg gatgccgatc gccgtctgcc ccacatgcag atgggtgttg
     3781 aacaggctga ccagctggtt caggtactgc agcaggttgt cgccgaagac cagcggatgc
     3841 gtcgcgccgt cgaccagttc gatcttgggc gcctcgacga tcaccttggt cgccgcctgc
     3901 accttgatct ggccgtcgtc gacgcggatc gtcaccaggt tggaaccgtt ggcgtccgac
     3961 agcgtcagcg tgtgcgcatc gtcgtccagc gacaccatca ggccctgctc gctgcgcagg
     4021 atcttcagcg gcggcttggc ggcggtcccc tgctcgtcct tgggcaggtc ggccgtgccc
     4081 caccagccgc cgctccagat cggacgcgag ggatcgccgg cctcgaactc gatccacacg
     4141 ccggcgccca ccggcggcac cgcgtgcagc ccgacgcccg gccccgcgta cggcgcgcag
     4201 ggcgtggccc acggcgtcac cacgtcggcg ccgaggacct cgggcacctg cgccttgacg
     4261 cggccgatcg ccgacgggtc ctggttgtcg gtcacgatgc cgcggtactt gccccagtgg
     4321 cgctggcccg cctgctgcgc cagccgcacc accagctgtt cgagcgtgcc ggtgtccatc
     4381 agaagactcc cgcgggcagg ccgccggcgg caccgccgga tcccgccgat cgcgcgttgc
     4441 gatgcagcga gaagcgctga cggtaggcgt cgtcgttgag cacatgggtg acgcggccga
     4501 tcaggtagtc gcccgacagg tagccgccga cgccgcgcac ccggatcacc tcatagggcc
     4561 gcagcacgcc ggtgtagcgg tcggcgtcaa gctcgccgct ggccgaccag gcccaggacg
     4621 acacgtccac cgccgcggcc gtggcggcgt ccaggtccgt ctgttcctcg cgcgtgccgg
     4681 tgagcagcgc gatgccgggc gtcatgcccg agacggcgtc ctcatgcgcg gcgacgtcgc
     4741 ccagcggcgc ctggctggcg tcggtggcgg tgctggacag caccgtgagg tcggccagcg
     4801 cgacgctgcc ggcctgcgcc tggcgcgggc gcagcgcgtc gtactcggcc gagaagcgcg
     4861 cgacgttgcg ctggtcgccg accagcagca gctccggcag cgtgcccggc gccggcgcca
     4921 gccgcttgaa cgcgccgacg ctcagccccg gcgcgtcgcc cgggcggacg aaggcgaaca
     4981 tcccgtggct gcgcgccagc tcccgcacca gctgcatgcc cgtgccgcgc tgcacgaccg
     5041 cgcgctgcag cgcgccgccc gccgccgaca ccgcgtccac gtccggcgtc aggccggcct
     5101 cggcgaacac gctgctgacg atgtcctgcg gcgccatgtc ctcgaacagc gcgaccttct
     5161 cgtcgcggtc cagcagcgcg ctgtcgtcat gcacgaccag cgtcatgcgg ctctggtccg
     5221 ggatcgcgct catctcgtag cggcggccga cgaccgggcc gtcgatcagc ggcacgaagt
     5281 cgccgtcggc gaccttcacc tcgatgcgca cgcgcgcgta gctttcctcg gccgcgagcc
     5341 agcggccctg cgtgtcggtg ctgaccggcc actcgagctc cgcctcggcc gcgatgccga
     5401 tgccctggtc gacgcggacc tcggtgaagc gctccagctg ctcctggctc gccggcgcgt
     5461 tgtcgaaata gacgcggtat tcggcgcgag ccatcgttca ccccttgggc acgtcgatcg
     5521 gacgcccggc cggccgcagc agcgaggcgg cctccagttc ggcgttcgcg tcggcgacat
     5581 gccagtagcg ggtcacgtcg ccgtagcgct gctcggccat cgcgtcgagc tggtcgtgcg
     5641 cgcgcaccgt gaccgcgtcg ccgccggacg acggcaacgg gcgcagcacg acggcggcga
     5701 cgtcctcgcc ggcgcggttc ttcgccgtca ccagcggcag tccggcgtag cgggagttct
     5761 tcaggaacat gcgttgcctc cggtggatgg ggtcagaagg gaatgatgtc cagcgcgaac
     5821 tcggccacct tggccaggtt cagcgtcgcc tgcgtgtcct tgaccatgcg cgagtactcg
     5881 taggcgccct gcccgaccgc gtcgtcggtc ggcgcgacgc tggagacctg cagcccgatg
     5941 cggacctcgg cctggatcgg attgagcagc acgtcgtact tctgctcggt gatgaccatc
     6001 gaggtgatcg tcaccggcag cacccgcgac gggccccaga tgaacaggag gcgcggcagc
     6061 gccaggcgcg ggatcggctg ggtcggcgcg gcggcgcccg ggccggagat cgccgaggcg
     6121 atcgcgtcga tcgccgcgcc gatcagcccg cccggcgtgc ccggcgggaa gaccatcttc
     6181 tccagcgcgg ccagctgcgg cccgacgccg aacacccgcg ggatcgcgga gaccgcgccg
     6241 ccggcgccga ggtcgtcggc ggcggagaac tgggccgtga tctcgaagct ctcggtcggc
     6301 ggcgccgacg cgcggtcgcg ctcggcctgc gcctgcgccg aggcgctctg cggcggctgc
     6361 gggaccgcga tcgtgcgggt catctggtcc ggattgaact ggaagaccac gatgttggga
     6421 atcggcccca gcaggttgat gccgtactcc accagcacgc cgcgcaacgg aagcaaggcc
     6481 atgtcagcgc tcctccagcg gcgaggccga ggacgacgtc gagggcggcg tcgacgtgga
     6541 ggtcgccatc gagctcggcg tgtcgccggg atcgcgcgag agccacagcg ccagccgcgc
     6601 ggcgatcagc ccggccacgg cacgggcgtc ggcgcccgcc ggcgcgtcga tgcggcccag
     6661 gtccagccgc gtcgcgccgc cgcgcgcggc gatgccgtcc agccgcgtca gcgcgtcgcg
     6721 cagcgcggcc tgcacctgag cggccacgtc ggcgccgagc gcgtcggccc cgtgccggtc
     6781 ggcggtgcgc agcgagacgt ccagtcgtcc gatctcgacg ttcatggcga ctcctccagc
     6841 agtggggcgt agcggccgaa ctgctggcag ccggcgacgc ggttcatctt ctcgagctcg
     6901 cgccgcacgg cggccagcac cgccggcatg tcgacgcgcg gcggcgcggc cgggtcgcgg
     6961 ccggcggcca gcaggcaggc gttgaaggcg gccgagcgga tgctgccgcc ggccagctcg
     7021 aactgctgcg ccagccagga cacgtccagc gacctcgcgt ccacccgcgc cgggaaggcc
     7081 tggcgccaga gccgttcgcg ctcggcggcg ccgggcatcg ggaactcgac gatgtggcgc
     7141 aggcggcgcg tgaacgcgtc gtccaggtcc ttgcgccggt tgctggccag caccgccagg
     7201 cccttgaagc gctccatgcg ctcgagcagg tagctgacct cgatgttggc gaaacggtcg
     7261 tgggcgtcgc ggacctcggt ccgcttgccg aagagcgcgt ccgcctcgtc gaagaacatg
     7321 aggcagtcgc cggtctcggc cgcgtcgaag atgcgacgca ggttcttctc ggtctcgccg
     7381 atgtacttgt tgacgacctg cgacaggtcg atgcggtaca tcggcagcga cagctcggac
     7441 gcgagcgcct ccgccgccat cgtcttgccg gtgccgggcg gcccgcagaa cagcaccgac
     7501 agtccgccct cgttccaggc gcgcgccgtg ccccagtcgt ggtgcacgcg ggcgagcgcg
     7561 cgcatggcca gcacgatctc gcgcagttgc cgcgcctgcg atggcggcag caccagctcc
     7621 tcgagggcga agcgcggctc gaccgcctgc gccaggccgt cgagttccag gcgcgcttcg
     7681 gcccggcaca gcgcgaacag cgcggacgcg ccggcatcgg gcggcaggcc ccgcgccgcg
     7741 gcggcgacgc gcgcgagcgg gcggtcggtc aggcggaaac ggcgcgcggc gtcggcgccc
     7801 gcggcacgca gggccggcgg cgcgtcggcc agcgcggcgt cgaaccgggc ggtgcgctcg
     7861 gcggtgtcgg gcggcgtcat cggccaagcc ggcaggagga gcgcggccgg cacgccacgg
     7921 ccgctcgggc cgccggtcga cgcggcactg ccggtgccgg ccgccccggc ggcggtcccg
     7981 tcctgcatcg gcagcatcca gcgcagcgga agcgcggcca gcggcgtcgt ccagccttcg
     8041 ccggcggcgg cgcagcagcg ttcctgccat tgctcgggaa gcagcaggtc ggcgccacgc
     8101 agccaggcca cggtcgccag cggcgcgagg ccgcgatcgg gcgtgacatc tccgtcgacg
     8161 accagcagcc cgcgcccgca ggcccgcgcg atggcccgcg cggcggcggc gaagtcggtg
     8221 ccgcgcggcc ccagcagcgg cagcacctgc atcgccacgg gcgggtcgct gcgcagccgc
     8281 gcgagccgcg cgcgctgcgc gtcggtgtcg tcgatcggcc ggacgaccgt cgcctgcgcg
     8341 gctccttcgg ccagggttcg ggctcgggtc tcgactcgcc cgtgggtgtc ggtcccctcc
     8401 tcgaccacgc ggagcagctc gtgcgcgacg cgcggcggca tctccagcgg ctgtgtccaa
     8461 tcgagcgcat cgcggcccgg cggcaggttc agcaatccat ggcggaacag cgcatggccg
     8521 gcgtccgcgc aggcggcgat ctcgaccggc tcgtcccaca ggcgctgcgc cagcgcgagc
     8581 gtcggccacg gccgcgtccc gtcgttcatg caggcggcgc agaccggccc gatcgccgcg
     8641 tcggcgcgcc ccatcagcgc gcaggccagc acgaactgcg cggccggcgt caactggcat
     8701 tcgcgcgcga tccagcgcca gtcgccacgc cgctcgggcg cacggtccag cgcgcgctcg
     8761 ggcaggtcga acgcggcaag gcgctcgtcg agataccgtg cggtcacgtc ggtctcgaag
     8821 aagcggcgct tgtcgtcgcg gaatcgcagc aggtccaggc tgtcctgcga cgcgtccccg
     8881 atcgccggca gctcgccggg cgcgcgcggc cacaggccgc gctgatgcca gcaccacgcg
     8941 acctcgcggc gcagccgcag cgtgacctgg gcgagccacc aggcgcaggc cgcgtcggcg
     9001 accgcgagat ccggattgag cgcgaccggc tcgaccagcg tcgaggggat cggcaaggcg
     9061 tgcggcgcgt tcatgacctc aggtccagtt gagctgcggc gtgtcgaacg cctgctggcc
     9121 gttgacgcgc aggatcacgc ggtactcggt gctgggcggc agcgcatggt cgtcgtcgac
     9181 aacgaaggtc agcgagttgg gcgtgccgcc gggctgcggc tcgagcagca ggcgcgactg
     9241 gccgtcgagg tacagcgcgg cgtacgccgc cgagttcggc ccgctgagct ggctcccgag
     9301 cacgacgaag ctgccggagc gcctggtgcc gaccaccgtg agcacgccgg cgaggttcac
     9361 cgcggtgatg cgggggctga gccgccccgt gaccgcgttg ctgctggtga gccggccgcc
     9421 cggcagcgcg cgcgcgacgc agacgggata ggcgccgggc tcgaccgcgt cggccgcggc
     9481 gatggtcgcg gtcaggccgc catcgggcgt cgcgacggcc gggtagacat gcgggccgat
     9541 ctggatctgg tcgtagccgg cgaagtcggt gccgcgcacg acgaacgcct ggccagcctc
     9601 gaagcgctcg ggctcgatcg ccgtggcgac ggcgccgagg ctgggcagca cggtcacgcc
     9661 gtcgtacatc cgcggcgcgg gcgccggctg gccgatcgtc ttgaccggcg gggcgtaggc
     9721 cggcggctcg tcggtcgagg ccaccatcac cggccgcacc tggaagccgg ccgacacccg
     9781 gtacttctcg tcgctgccct gcatcacgcg ggacagcatc tcgacatcgg cctcgtccag
     9841 cgtgatgcgc agtggatcgg ggctcgacgc gagcggcagc tgggctgcgc ccggcttgag
     9901 cacggacagc gcgttcaggc cgcggatgcc ccggcccagc agccggtgcg cggcgatgct
     9961 gtcgctgtcc gggccgtcga aggccgtcag caggtagtgc agcaccagcc acaccggcgg
    10021 cgcctggccg gcgtccagcg cccggttgcg caggtcgccg tcgacggcga cgcggtacag
    10081 gaacaggttg aggctgccct ggcccgactg cgccgccttg gcggcctcct cgggccggcc
    10141 gacggtgatc gcggaaatgc cggacgcggt ggccagcgcc gtcttcagca ggtcgctgac
    10201 ggcgccgatc gcgtcccggc tgtcggccag cgacatggtt cacccccacc gcaaataatg
    10261 gcgccgcgcg gagaagcgcg gcggggccgg cgcggccgcg gggatcggcc ccggctgcgg
    10321 gaccggcggc gcggcgggcc gcgatggcgg cggggcgaca tggacctcca gcgcgatctg
    10381 gccgatgcgg atccgaggcg cggcggcgcc tcccgtcatg cgcgaggccg cgcccgtcgt
    10441 cagcgtgtcg atggcggcct cgttgcgcgc ggcgacggcg cgccgggtct cggccgacgt
    10501 gcccgcgccg gctcgaccgt cctcccacgc ctccgattcc gtcgctcgcc cctcggcccg
    10561 cacctgcggc tgcggctgca cggagaaggc ttccgctgcc tcgatcacgt cctgtgatgc
    10621 cgccgcggaa gactcgtcgg accttgtcga tgccgctgcg atcgccgggg ccggcgtctc
    10681 gaaggacgtc gacgaggccg ttgtcgtcgg gacctgcggg gcgtgcgcca tatcgacgtc
    10741 gtctcgacct ccgcggcgat cgaccggctc ggccgccatg cccggcatgc gcccgggctc
    10801 gacgatccca ggcctcgcat cgcgcgcggt cgacgccgcg gtcgatgcgt cgcgcgaggc
    10861 ggcggtcaag ccgttcgtcg atggctccgg cgatgtgccc gcgaacgcgt cgatggacga
    10921 cgcgacggat cgggtcgccg tgtgcgtggc ggacctcgcg atgtcctcgt ccaccgcgag
    10981 cgctcgcgcg gcatgcggtc cgcgctgcgt gtccatcggg aacgatgcat cgctcgacct
    11041 cgaccggagc gtgccgcgcg cattcgacga cacatcgagc gcgctgctcg gcaccacgcc
    11101 gggcgacatc cgcgaggtct gatcggaagc gttgtcccgc gccgtcgaca cttgaaggga
    11161 agcggcctcg cgcggcgctg ccatcgccat gaacgacgat gaatgcggcg accgcgccga
    11221 cgatgatcgg gccgtccgcg gcgcggcctg ggccgcgagc ctggcgaaat agccgctcat
    11281 gcccagccct ccacctggtc cagccgccgc aggtaatgcg cccggcgcca ggccggcagc
    11341 gacaggatgt ccgcctcgct ccaatggaag gcggaagcga gccggacgac ctcgtccatc
    11401 agcccccgct gctgccgcgc gaaaccggcc agcagcgccg cttcgaggtc gcaggcgacg
    11461 tcgttgggcc ggccgcagtg cgggcacgcc gcatgcagct tcagcgcggt cagcggatcg
    11521 tgtgcctcat agatcgtgtc cagcgccgcc agcagcgggg ccgggcacgg ctcgccgtcg
    11581 gccagcggcc ggccgtcgac cgactcgatc agcgtcaccg cgatctgttc cggcgacgcg
    11641 acgcggccag gccacgacga caggtcgtcc ccgcacggca gccgca
//
LOCUS       DATCRO010000133         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_327839, whole genome shotgun sequence.
ACCESSION   DATCRO010000133 DATCRO010000000
VERSION     DATCRO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327839"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VIN75_03675"
     CDS             <1..923
                     /locus_tag="VIN75_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973291.1"
                     /translation="QILYVGTSQGLLAYDPAAASWVAVSPGTLAMRVSALGLGADGLF
                     AGTDDGVFALPLGRNPAGVTPVAAGLAGSTVRSLLVTPSVVLAGTVDANDDNHVLFTS
                     EAAAAQGTGLWQAFGVGSAGTRRITSLLMVDTNLLAATNGNLVLVASAASAWTSANTS
                     ANPAQQISDPFGAVTSLYSDGTTIFASTGSQGVFVSPVGTSFVWTPYAGSAPTVLPSM
                     EVRQLRPGDGVLYASTRAGVAVTADPVLSSTPPASTPSSPSPGGSGSGGGASSPWWLL
                     ALLLAGAALRRHQNAFQGPSVDRAWPQIDV"
     gene            complement(866..2194)
                     /locus_tag="VIN75_03680"
     CDS             complement(866..2194)
                     /locus_tag="VIN75_03680"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY8973292.1"
                     /translation="MSCTDILRRATAAVLVAALSACGGSSSTTTLTGPGLGDGSSVTA
                     AHQDAPTGDNTTQIVVDAGPASSFAIGAVNVPYVTVTVCAPGSAGRCVVVDHVFLDTG
                     SVGLRLLRSKVADLALPPVDVPADAASGTPAGAALECYPFVLGAVWGPMATADLQIAG
                     ERAASLPVQLIDDSTTPAHAAPADCVAASNGQLMNSAATLQANGILGIGMIAFDCGQT
                     CVAGDYSGGYTLYWSCASSCAPAAMPAALQVQNPVTYFATDNNGTLIALPRLPEFGAG
                     TVTGRLVFGIGTQANNQIPSTATIVHVDANPADATYLYFTTKVAAINYPDSYIDSGSN
                     AYFFDDASLTLACQNSTGGASSWYCPATALQRTAVVSDKAGASASIDYEVVNADALFA
                     TSSLGLDDLAGSISGGSPSFVWGLPFFYGRTVYTSIWGQALSTDGPWNAF"
     gene            complement(2181..2642)
                     /locus_tag="VIN75_03685"
     CDS             complement(2181..2642)
                     /locus_tag="VIN75_03685"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEY8973293.1"
                     /translation="MTAARIGAGIVLLLAAGRAGATLGEPAATVDADGALLKGSHRVA
                     QSQAARLQVHTITLADGSAVREYVAPSGIVFAVAWSTRLKPRLETLLGSHAPAYAAAA
                     SAALARPGVRHGVSLSSGDLVVQASAHLNAHAGLAYLKSLVPDGVRIDELH"
     gene            complement(2639..>3809)
                     /locus_tag="VIN75_03690"
     CDS             complement(2639..>3809)
                     /locus_tag="VIN75_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973294.1"
                     /translation="PRDAQVLADYADALAVANGRTLAGELTQEEFDTQQGTIAEALLA
                     HGAAGGRATAAQASRPRARLLAAGALLAALVAGAWQAWLGRPDNPTPARATLAQPGAA
                     SAPAPAAHALSDEQLEHMVAQSLQRVQKAPQDAAAWAMLAHSYDMLGKFAESSKAYAT
                     LARLVPRDAQVLADYADALAVANGRTLAGEPTVLVNKALEIDPRNVKALTLAGSAAFE
                     RQDFAEAVARWQAARDLARDPRFASEIDASLATARASAKGQAAPARAAASARALAPAS
                     AVVSGRITLADDLQSKVPPDATVFVFARPIDGSRMPVAVLRRKASDLPLQFSLDDSMA
                     MVPDARLSRVSTVVIGARISRRGDIAPQPGDMQGLSAPVSVGTRGIKVEISEVLQ"
BASE COUNT          484 a         1350 c         1453 g          522 t
ORIGIN      
        1 accagatcct gtacgtcggc acgtcgcagg gcctgctggc ctacgacccc gccgccgcga
       61 gctgggtcgc tgtcagcccc ggcacgttgg cgatgcgcgt gagcgcgctc gggctgggcg
      121 ccgacgggct cttcgccggc accgacgatg gcgtcttcgc gctgccgctc ggccgcaacc
      181 cggcgggcgt gacgccggtg gcggcgggcc tggccggcag caccgtgcgc tcgctgctcg
      241 tgacgccgtc cgtcgtgctg gccggcacgg tcgacgcgaa cgacgacaac catgtcttgt
      301 tcaccagcga ggcggccgcc gcgcagggca ccgggctgtg gcaggccttc ggtgtgggct
      361 cggcgggcac gcgccgcatc accagcctgc tgatggtgga caccaacctg ctcgcggcca
      421 ccaatggcaa cctggtgctg gtcgcctccg cggcctcggc ctggacgtcc gccaacacga
      481 gcgcgaaccc ggcccagcag atctcggacc cgttcggcgc ggtcaccagc ctgtacagcg
      541 acggcacgac gatcttcgcg tcgaccggca gccagggcgt cttcgtgagc cccgtcggaa
      601 cgagcttcgt ctggacgccg tacgccggct cggcgccgac cgtgctgccg tcgatggagg
      661 tgcgccagct gcgtcccggg gatggcgtcc tctatgcgtc gacgcgcgcc ggcgtcgccg
      721 tcacggccga tccggtgctg tcctcgacgc cgcctgcgtc cacgccgtcg tcgccctcgc
      781 cgggcggctc gggttcgggc ggcggagcgt cgtcgccgtg gtggctgctc gcgctgctgc
      841 tggccggcgc ggcgttgcgc cgtcatcaga acgcgttcca ggggccgtcg gtggacaggg
      901 cctggcccca gatcgacgtg tagaccgtgc ggccgtagaa gaacggcagg ccccagacga
      961 agctcggcga gccgcccgag atcgagcccg cgagatcgtc cagcccgagg ctggaggtgg
     1021 cgaacagcgc gtcggcattg accacctcgt agtcgatgga tgcgctcgcg ccggccttgt
     1081 ccgagacgac ggccgtgcgc tgcagggccg tcgccgggca gtaccagctc gacgcgccgc
     1141 cggtgctgtt ctggcatgcc agcgtcaggc tggcgtcatc gaagaaatag gcgttggagc
     1201 cgctgtcgat gtagctgtcg gggtagttga tcgccgcgac cttcgtcgtg aagtagaggt
     1261 aggtcgcgtc ggccggattg gcgtcgacgt ggacgatggt cgcggtggag gggatctggt
     1321 tgttggcctg cgtgccgatg ccgaacacga gccggcccgt gacggtaccg gccccgaact
     1381 cgggcagccg gggcagggcg atcagcgtgc cgttgttgtc ggtggcgaaa tacgtcaccg
     1441 ggttctgcac ctgcagggcc gccggcatgg ccgcaggcgc gcagctggac gcgcacgacc
     1501 agtacagcgt gtagccgccg ctgtagtcgc ccgcgacgca ggtctggccg caatcgaagg
     1561 cgatcatgcc gatgcccagg atgccgttgg cctgcagcgt ggcggcggag ttcatcagct
     1621 ggccgttcga cgcggccacg cagtcggcgg gcgcggcgtg cgcgggcgtg gtcgagtcgt
     1681 cgatcagctg caccggcagc gaggcggcgc gctcgccggc gatctgaagg tcggcggtcg
     1741 ccatcggccc ccatacggcg cccagaacga acggatagca ctcgagcgcc gcgccggcag
     1801 gcgtgccgct ggcggcatcg gcgggcacgt cgacgggcgg gagcgccagg tcggccacct
     1861 tgctgcgaag cagccgcagg cccaccgagc cggtgtccag gaagacgtgg tcgaccacca
     1921 cgcagcggcc ggcggagccc ggcgcgcaca ccgtcacggt cacgtagggc acgttgacgg
     1981 cgccgatggc gaagctgctg gcggggcccg cgtcgacgac gatctgcgtg gtgttgtcgc
     2041 cggtcggcgc gtcctggtgc gccgcggtga cgctgctgcc gtcgccgagg ccgggtcccg
     2101 tgagcgtcgt cgtcgacgag gatccgccgc acgcggacag cgcggcgacg aggacggcgg
     2161 cggtcgcccg gcgcaggatg tcagtgcagc tcatcgatgc ggactccgtc gggcaccagc
     2221 gacttcaggt aggcgaggcc ggcatgcgcg ttgaggtggg cactggcctg cacgacgagg
     2281 tcgccgctcg acagcgatac cccatgccgt acgcccggcc tcgccagcgc ggcgctcgcc
     2341 gcggcggcgt aggccggggc atgggatccc agcagcgtct ccaggcgcgg cttcaggcgc
     2401 gtgctccagg ccacggcgaa gacgatgccg ctcggggcga cgtattcgcg gacggcggag
     2461 ccgtcggcca gcgtgatcgt gtggacctgc aagcgcgcgg cctgcgattg cgcgacgcga
     2521 tggctgccct tgagcagcgc gccgtcggca tcgacggtcg cggcgggctc gccgagcgtc
     2581 gcgccggccc ggccggcggc cagcaacagc acgatcccgg cgccgatgcg cgcggcggtc
     2641 attgcagcac ctcgctgatc tccaccttga tgccgcgcgt gccgacgctc accggcgcgg
     2701 agagcccctg catgtcgcct ggctgcggcg cgatgtcgcc gcgccgcgag atccgggcac
     2761 cgatcaccac ggtcgacacc cgggacaggc gcgcgtcggg caccatcgcc atcgagtcgt
     2821 cgagcgagaa ctgcagcggc aggtcgctcg ccttgcggcg caggacggcc acgggcatgc
     2881 gcgagccgtc gatgggccgg gcgaacacga acaccgtggc gtccggcggc accttggact
     2941 gcaggtcgtc cgccagcgtg atccgcccgg agacgaccgc gctggcgggc gccagggcgc
     3001 gtgcgctcgc cgcggcccgc gcaggcgcgg cctgaccctt ggccgacgcg cgagccgtcg
     3061 ccaggctggc gtcgatctcg ctggcgaagc gcggatccct ggcgaggtca cgcgcggcct
     3121 gccaccgcgc gacggcctcg gcgaagtcct gccgctcgaa cgccgcggag cccgcgagcg
     3181 tcagcgcctt gacattgcgc ggatcgatct cgagcgcctt gttcaccagc accgtgggct
     3241 cgccggcgag cgtgcggccg ttggcgaccg ccagcgcgtc ggcatagtcg gccagcacct
     3301 gggcatcgcg gggcaccagg cgcgcgagcg tcgcatacgc cttgctggac tccgcgaact
     3361 tgccgagcat gtcgtaggag tgggccagca tcgcccacgc ggcggcgtcc tgcggcgcct
     3421 tctgcacgcg ctggagcgac tgagcgacca tgtgttcgag ctgctcgtcg ctcagggcat
     3481 gcgccgccgg cgcgggcgcg ctggcggccc cgggctgcgc cagcgtggcg cgggccggcg
     3541 tggggttgtc cggtcggccc agccaggcct gccaggcgcc ggcgacgagc gcggcgagca
     3601 gcgccccggc ggccaggagc ctggcgcgcg ggcggctcgc ctgcgcggcg gtcgcgcggc
     3661 cgcccgccgc gccgtgggcc agcaacgcct cggcgatggt gccttgctgc gtgtcgaact
     3721 cctcctgcgt cagctcgccg gccagcgtgc ggccgttggc gacggccagc gcgtcggcat
     3781 agtcggccag cacctgggcg tcgcggggg
//
LOCUS       DATCRO010000134         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1092923, whole genome shotgun sequence.
ACCESSION   DATCRO010000134 DATCRO010000000
VERSION     DATCRO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092923"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VIN75_03695"
     CDS             <1..774
                     /locus_tag="VIN75_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902633.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEY8973295.1"
                     /translation="TFNDAHLRALGRVHDAAQARAAAEEAARSFETFNLDLMYALPGQ
                     TLADLDADLDLAFALGAPHLSLYHLTLEPNTVFAQRPPANLPDDDTAYDMLDRVTERT
                     ASGGFERYEVSAYAKDGHRCRHNVNYWQFGDYLGLGAGAHGKLSFAHRVTRQVRWREP
                     NMYMERTLAGQGVSNESDVARAQLPFEFMLNALRLREGFALTLFTERCGLPLSAIAKP
                     LDEAERKGLIERTGAPGAEWIRPTERGFDFLNDLQELFL"
     gene            1082..>3451
                     /locus_tag="VIN75_03700"
     CDS             1082..>3451
                     /locus_tag="VIN75_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY8973296.1"
                     /translation="MPENAAIDADQFNRIARRNVTLPLYTGIISVGAFLLFVWYFMVV
                     NGWVDHTHQVIGKLSEVTTLQSDMESATRGYVITSDEAFLAPYQVARPQMDSLLAELV
                     RETADNPSQTERLRRVRSLQQAWEAFADKTIAARHDGGDAGAGVKSLTGKQLFDRVRT
                     ELTAARGTEQGLLNDRLEELRMVTWLGIGTYVLIILMAKGYIAWAGRRDIRQLSEDFG
                     EALQAQTAAADALTEQAWLRDGQAQLAETLIGQQTLTTVGRSVLAFCGRYLDPVVAAL
                     YVRDDTGTLQRVSTHGFDGQDDDGQVQAARDGLVGQAVRDGRVNHVTHLQGSYFKVGS
                     GLGATSPAELVIAPISNDGVVNGVIELGFMRHVTSRDLELLKLLRGAIGASVDSAMYR
                     KRLQDSIEETQQLNEELQVQQEELRTANEELGEQSRVLTESQSHLERQQAELEATNTQ
                     LAEQADALDRKNEALNAAQVELQQRADELQRASRYKSEFLANMSHELRTPLNSSLILA
                     KLLSENKEGNLSAEQLKFAQTIYSAGNDLLNLINDILDLSKVEAGRLELQPQFVSVHR
                     MVDALQRLFAPLAGEKKLAFEVQVAPEAPTSMVTDNLRLEQILKNLLSNAVKFTETGG
                     VTLAVQARPGDRVAFSVKDTGIGIPEGQQDIIFEAFRQADGTINRRYGGTGLGLSISR
                     ELARLLGGTIEVDSAAGQGSTFTLTLPAKWTDVPEAEPAPPAPADAPVRPAAVASASP
                     RPGADAPVAAPESLPAPRRVADDRDEAVPAKGRVLLVVEDDVDFAGVLYN"
BASE COUNT          571 a         1224 c         1203 g          453 t
ORIGIN      
        1 accttcaacg acgcccacct gcgcgcgctc gggcgcgtgc acgacgcggc gcaggcgcgc
       61 gcggcggccg aggaggcggc gcgcagcttc gagaccttca acctcgacct gatgtacgcg
      121 ctgcctggcc agacgctggc cgacctcgac gccgacctcg acctcgcgtt cgcgctcggc
      181 gcaccgcacc tgtcgctgta ccacctcacg ctggagccca acaccgtctt cgcgcagcgc
      241 ccgccggcca acctgcccga cgacgacacg gcctacgaca tgctcgaccg cgtcaccgag
      301 cgcacggcgt cgggcggctt cgagcgctac gaggtctcgg cctacgccaa ggacggccac
      361 cgctgccgcc acaacgtcaa ctactggcag ttcggcgact atctcggcct gggcgccggc
      421 gcgcacggca agctgtcgtt cgcgcatcgc gtcacgcgcc aggtgcgctg gcgcgagccg
      481 aacatgtaca tggagcggac gctcgcgggc cagggcgtgt ccaacgagtc cgacgtcgcg
      541 cgcgcccagc tgccgttcga gttcatgctc aacgcgctgc gcctgcgcga gggctttgcg
      601 ctgacgctgt tcaccgaacg ctgcgggctg ccgctgtcgg ccatcgccaa gccgctggac
      661 gaggcggagc gcaaaggctt gatcgagcgc accggcgcgc cgggcgccga atggatcagg
      721 ccgaccgagc gcggcttcga cttcctcaac gacctgcagg aactgttcct gtagggcggg
      781 ttagccgcag gcgtaacccg ccatttgacg gcgcgctgca atgcggcgag caatcacggc
      841 gacggatcgg aacgccgatg gacggatcgg aacgccaatg aacggcgggt tacgcctgcg
      901 gctaacccgc cctacaaggc cgggtcattg cgggcacgtc gattgccatg gggcgaccaa
      961 tggtcgagca ggcatcccgg aggcggcgcc gcgccgccgc gtccggggca aaccccgccg
     1021 gatcggtgac gcgctcaaac tatgatcgcg ctcgcccgac cgacccgctg ccaggacacc
     1081 catgcccgaa aacgccgcca tcgacgccga ccagttcaac cggatcgcgc gccgcaacgt
     1141 cacgttgccg ctgtacaccg gcatcatcag cgtcggggcc ttcctgctgt tcgtctggta
     1201 tttcatggtg gtcaacggct gggtcgacca cacgcaccag gtcatcggca agctcagcga
     1261 ggtcaccacc ctgcagtccg acatggagtc ggccacgcgg ggctacgtga tcaccagcga
     1321 cgaggccttc ctggcgccgt accaggtggc caggccgcag atggactcgc tgctggcgga
     1381 gctggtgcgc gagacggccg acaacccgag ccagacggag cgcctgcgcc gagtccgatc
     1441 cttgcagcag gcctgggagg cgttcgcgga caagacgatc gccgcgcggc acgatggcgg
     1501 cgacgccggc gccggcgtga agtcgctcac cggcaagcag ctgttcgatc gcgtccggac
     1561 ggagctgacc gcggcgcgcg gcaccgagca gggcctgctg aacgaccgcc tggaagaact
     1621 gcggatggtg acctggctgg gcatcggcac gtacgtcctg atcatcctca tggccaaggg
     1681 ctacatcgcc tgggccggcc ggcgcgacat ccggcagttg tccgaggact tcggcgaggc
     1741 gctgcaggcc cagaccgccg cggccgacgc gctgaccgag caggcctggt tgcgcgacgg
     1801 ccaggcgcag ctggcggaga cgctgatcgg ccagcagacg ctgaccaccg tcggccgcag
     1861 cgtgctggcc ttctgcgggc gctacctcga ccccgtggtg gcggcgcttt acgtgcggga
     1921 cgacacgggc acgctgcagc gcgtgtccac ccacggcttc gacggccagg acgacgacgg
     1981 ccaggtgcag gccgcgcgtg acggcctggt ggggcaggcg gtgcgagacg ggcgcgtgaa
     2041 ccacgtcacc cacctgcagg gcagctactt caaggtgggc tccggcctgg gtgcgacgtc
     2101 gccggccgag ctcgtgattg cgccgatcag caacgacggc gtcgtcaacg gcgtgatcga
     2161 gctgggcttc atgcgccacg tgacgtcgcg cgacctcgag ctgctcaagc tgctgcgcgg
     2221 cgcgatcggc gcgtcggtgg attcggcgat gtaccgcaag cggctgcagg actccatcga
     2281 ggagacccag cagctcaacg aggagctgca ggtgcagcag gaggagcttc gtaccgccaa
     2341 cgaggaactg ggcgagcagt cgcgcgtgct gacggagtcg cagtcgcacc tggagcgcca
     2401 gcaggccgag ctggaggcca ccaacacgca gctggccgaa caggccgatg cgctcgatcg
     2461 caagaacgag gcgctcaacg cggcgcaggt cgaactgcag cagcgcgccg acgagctgca
     2521 gcgcgcgagc cgctacaagt ccgagttcct ggccaacatg tcccacgagc tgcgcacgcc
     2581 gctgaacagc tcgctgatcc tggccaagct gctcagcgag aacaaggaag gcaacctgtc
     2641 ggccgagcag ctgaaattcg cccagacgat ctacagcgcg ggcaacgacc tgctcaacct
     2701 gatcaacgac atcctcgacc tgtccaaggt ggaggccggc cgcctcgagc tgcagccgca
     2761 gttcgtgtcg gtgcatcgca tggtcgacgc gctgcagcgg ctgttcgcgc cgctggccgg
     2821 cgagaagaag ctggcgttcg aggtccaggt cgcgcccgag gcgcccacgt cgatggtgac
     2881 cgacaacctg cggctcgagc agatcctgaa gaacctgctg tccaacgcgg tcaagttcac
     2941 cgagaccggc ggcgtcacgc tggccgtgca ggcgcgcccg ggcgaccgcg tcgcgttctc
     3001 ggtcaaggac accggcatag gcatccccga gggccagcag gacatcatct tcgaggcctt
     3061 ccgccaggcc gacggcacca tcaaccggcg ctacggcggc acgggcctgg ggctgtcgat
     3121 ctcgcgcgag ctggcgcgcc tgctgggcgg caccatcgag gtggacagcg ccgcgggcca
     3181 gggcagcacg ttcacgctga cgctgccggc caagtggacg gacgtgcccg aggccgagcc
     3241 ggcgcccccc gcgcccgcgg acgcgcccgt gcgccccgcg gcggtcgcgt ccgcgagccc
     3301 gcgcccgggc gcggacgcgc cggtggccgc gcccgagagc ctgcccgcgc cgcgccgggt
     3361 cgccgatgac cgcgacgagg cggtgcccgc caagggccgc gtcctgctgg tggtggagga
     3421 cgacgtcgac ttcgccggcg tgctctacaa c
//
LOCUS       DATCRO010000135         6973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_890326, whole genome shotgun sequence.
ACCESSION   DATCRO010000135 DATCRO010000000
VERSION     DATCRO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890326"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1470)
                     /gene="fahA"
                     /locus_tag="VIN75_03705"
     CDS             complement(145..1470)
                     /gene="fahA"
                     /locus_tag="VIN75_03705"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEY8973297.1"
                     /translation="MTAIDFTHDAQAHSWIASANAAATDFPIQNLPFGRFRRAGSKDA
                     LRAGVAIGREVLDLHALGQLDPQIAVLLGPLAHGDLNGFMGQGRAACIALRHALFNGL
                     SAQPSGTASLWRARADALLVPQDEAEMALPCRIGDYTDFYTGIHHATTVGKLFRPDNP
                     LLPNYKWVPIGYHGRSSSIGVSGQSFPRPWGQVKGDGDAPVFTPSRRLDYELELGFFV
                     GPPNASGTPLDMAQAEASLFGATLLNDWSARDVQAWEYQPLGPFLAKNFATTISPWIV
                     TMEALAPFRTPFARDAADPQPLPYLDSAANRERGGIAIALEVWIQTHAMRARGESAVR
                     VSASSFADAYWTPAQLLAHHASNGCNLQPGDLMGSGTQSGPAANQGGSLLELSQGGRQ
                     PFDLGNGETRTFLQDGDCVILRGFCEAEGARRIGFGDCAGIVLPAIERA"
     gene            complement(1467..2789)
                     /gene="hmgA"
                     /locus_tag="VIN75_03710"
     CDS             complement(1467..2789)
                     /gene="hmgA"
                     /locus_tag="VIN75_03710"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8973298.1"
                     /translation="MTGTVSPNDGRHSGFGNHFSSEAVPGALPQGRNSPQKAPFGLYA
                     ELLSGTAFTAPRHGNQRTWLYRRQPSVRSGAYAPHAHALLKTGARDGVDIPPDPLRWH
                     PVPLAPEPLDFVDGLRTMVLNGDPDAQQGVAVHLYGAGKPMGNRAFVDVDGELLLIPQ
                     EGAIRVTTELGGLQAAPGEIVLLPRGMLFKVDPAADGHAVRGYVCENYGAAFRLPELG
                     PIGSNGLANTRDFIAPVAAYDDGPAQPWELIRKFGGALWRAPAAASPFNVVAWHGNLV
                     PYVYDTRRFNTIGSVSFDHPDPSIFTVLTSPSDTPGWANCDFVIFPPRWMVAEDTFRP
                     PWYHRNVMSECMGLVYGQYDAKEEGFRPGGLSLHNSMVPHGPDADAFDKASAADLAPR
                     KLDNTLAFMVESRWRLRPTAFALNGGHLDKSYADCWAPLQDNFKVDRP"
     gene            2882..3739
                     /locus_tag="VIN75_03715"
     CDS             2882..3739
                     /locus_tag="VIN75_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8973299.1"
                     /translation="MKAPAQPRPARESDSRGIQSIEVGGRLLLALAHHGQPLALKDLA
                     AAADMTAGKAHPYLVSFIKLGLVERETEGGRYGLGPLALQMGLIGLQQYDPVRLATER
                     IDELARATGHTVAIAVWGHRGPTLVRIAEAPSPVHVSMRHGTVMSIPDTASGRLFAAF
                     GPADAVREALANEARLAGAGAATRPARARAGGRFGLGAAFDREIDRVRADGIAGIDGV
                     ALPGLSALAAPVFDARGQLVLSLTAIGPSATFDAAVDGAVARRLKPAAAQLSTRLGWK
                     PGAPPGGAR"
     gene            complement(3810..4592)
                     /locus_tag="VIN75_03720"
     CDS             complement(3810..4592)
                     /locus_tag="VIN75_03720"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY8973300.1"
                     /translation="MIVTNLHKEPVALDTVLHRDLRLKNELNAIPRLAPFTSFIVSVS
                     EFADASLTFPILFVRAAPDALGKDTVAPVAVFGLKPGENLFATADGKWDASYVPAMLR
                     AYPFTMARIEGSDRWAMVFDNTWEGMSRTEGLPLFNENGEATELLNGVHKFVQDLETD
                     LERTRQACAALLELKLLKPMRFDATLASGEQLSVDGFMTADEEAIGKLSDAQIAQLYR
                     NGLLGLIQIHITSLNNMRRLLDRRIIQQNAAARAANDGAATA"
     gene            complement(4589..5896)
                     /locus_tag="VIN75_03725"
     CDS             complement(4589..5896)
                     /locus_tag="VIN75_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8973301.1"
                     /translation="MDVALLFFLILLNAAFAMSEMALTASRKARLQVMLESGEHGAEA
                     AIDLHDHPTKFLSTVQVGITSISVLNGIVGDAAFSEPLGEWLVHSFGLAQRAAHLSAT
                     ALVVLIITVITIIFGELVPKRIGQMFPETVASLLAPPMLWLSMATRPLVVLLSKTTEF
                     ILGLLGIRGNARREVTEEEIAASLEEGLDAGVIEAQEHQMVRNVFRLDDRQIGSMMIP
                     RAEITWLDANAPIAEVLRLVAAEEDGHSRYPVCRGGLDDVIGVITAQGLLKPLAEGRM
                     PVLTEELEPPVFVPETLSGMELLDHFRSHVAQLVFVVDEYGAVQGVITLRDVLEAITG
                     EFGPESGDDAWAVRREDGSWLMDGLIPVPEIKDRLEIKDLPEEDRGRYNTLAGMIMLL
                     LGRLPRTADVVEWGDWRFEVVDLDGKRVDKVLVSRNPSPGEQP"
     gene            6031..6849
                     /locus_tag="VIN75_03730"
     CDS             6031..6849
                     /locus_tag="VIN75_03730"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY8973302.1"
                     /translation="MDYLIGDLQGCCDPLDHLLATIDFSPSRDRIHLLGDLVNRGPQS
                     LPVLQRLAALGDAAQCLLGNHDLHLLAVAHGVRKASKGDTLDPILAHEDRDALLDWLR
                     HRRMAAEACGWLLVHAGVIPQWSATDTLAHAAEIEALLRSDDILQFLPTMYGNEPRRW
                     DPALQGPDRWRFIVNVLTRMRLCSAEGELELKKETPGEADPRMQRWFSHPNRLTRDVP
                     IAFGHWSQLGLVDTPTLLCIDTGCIWGGQLTAVRVDGGRREVIQVPCPQARKPG"
     gene            6884..6971
                     /locus_tag="VIN75_03735"
     tRNA            6884..6971
                     /locus_tag="VIN75_03735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6918..6920,aa:Ser,seq:tga)
BASE COUNT          998 a         2477 c         2476 g         1022 t
ORIGIN      
        1 accgcgactt ccgacgttcg gtgtcaggtc tcgcctgtcg cacgttcagc gccccggccc
       61 acggctgacc gcctggcgtg caacacgcaa gaccagacac ctcggcgcct cgcaggtcac
      121 cgttgcgatg cgccgcgatg cttcctacgc ccgttcgatc gccggcagca cgattcccgc
      181 gcaatccccg aacccgatgc gccgcgcgcc ctcggcctcg cagaagccgc gcaggatcac
      241 gcagtcgccg tcctgcagga aggtgcgcgt ctcgccgttg cccaggtcga acggctgcct
      301 gccgccctgg ctcagctcca ggagcgagcc gccctggttg gcggccgggc cgctctgcgt
      361 gccgctgccc atcaggtcgc cgggctgcag gttgcagccg ttgctcgcgt gatgggccag
      421 cagttgcgcg ggcgtccagt aggcgtcggc gaacgagctg gccgaaacgc gcaccgccga
      481 ctcgccgcgg gcgcgcatcg cgtgcgtctg gatccacacc tccagcgcga tggcgatgcc
      541 gccgcgctcg cggttggcgg ccgagtcgag gtagggcagc ggctgcgggt cggcggcgtc
      601 gcgcgcgaac ggcgtgcgga acggggccag cgcctccatc gtgacgatcc acggcgagat
      661 cgtggtggcg aagttcttgg ccaggaacgg gcccagcggc tggtactccc aggcctgcac
      721 gtcgcgcgcc gaccagtcgt tgagcagcgt ggcgccgaac agcgaggcct cggcctgcgc
      781 catgtccagc ggcgtgccgg acgcgttggg cgggccgacg aagaaaccca gctccagctc
      841 gtagtcgagg cggcgtgacg gcgtgaacac cggcgcgtcg ccgtcgccct tgacctggcc
      901 ccacgggcgc gggaagctct gcccgctgac gccgatcgac gagctgcggc cgtggtagcc
      961 gatgggcacc cacttgtagt tcggcagcag cgggttgtcg ggccggaaca gcttgcccac
     1021 cgtggtcgcg tgatggatgc cggtgtagaa gtcggtgtag tcgccgatgc ggcacggcag
     1081 cgccatctcg gcctcgtcct gcggcacgag cagcgcgtcg gcccgcgcgc gccacagcga
     1141 cgccgtgccc gagggctgcg ccgacaggcc gttgaacagc gcgtgccgca gcgcgatgca
     1201 cgccgcccgg ccctgcccca tgaagccgtt caggtcgccg tgcgcgagcg gccccagcag
     1261 caccgcgatc tgcggatcga gctggcccag cgcgtgcagg tcgagcacct cgcgcccgat
     1321 cgccacgcct gcgcgcagcg cgtccttcga gccggcgcgg cggaagcggc cgaacggcag
     1381 gttctggatc gggaagtcgg tggcggcggc gttggcggag gcgatccagc tgtgcgcctg
     1441 cgcgtcgtgg gtgaagtcga tcgcggtcac gggcggtcca ccttgaagtt gtcctgcagc
     1501 ggggcccagc aatcggcgta cgacttgtcc aggtggccgc cgttcagcgc gaacgcggtc
     1561 gggcgcaggc gccagcggct ctccaccatg aaggccaggg tgttgtcgag cttgcgcggc
     1621 gcgagatcgg cggcgctggc cttgtcgaac gcgtcggcgt cggggccgtg cggcaccatg
     1681 ctgttgtgca gcgacagccc gccgggccgg aagccctcct ccttggcgtc gtactgtccg
     1741 tagaccaggc ccatgcactc gctcatcacg ttgcggtggt accagggcgg gcggaaggtg
     1801 tcctcggcca ccatccagcg cggcgggaag atcacgaagt cgcagttggc ccagcccggc
     1861 gtgtcggacg gcgaggtgag caccgtgaag atcgacggat cgggatggtc gaagctcacc
     1921 gagccgatgg tgttgaagcg tcgcgtgtcg tagacgtagg gcacgaggtt gccgtgccac
     1981 gcgacgacgt tgaacggcga tgccgccgcc ggcgcgcgcc agagcgcgcc gccgaacttg
     2041 cggatcagtt cccacggctg cgcggggccg tcgtcgtacg cggccaccgg ggcgatgaag
     2101 tcgcgcgtgt tggccaggcc gttggagccg atcgggccca gctcgggcag gcggaatgca
     2161 gcgccgtagt tctcgcagac gtagccgcgc acggcgtgcc cgtccgccgc cgggtcgacc
     2221 ttgaacaaca tgccgcgcgg cagcagcacg atctcgcccg gcgcggcctg cagcccgccc
     2281 agctcggtgg tgacgcggat cgcgccttcc tgcgggatca gcagcagttc gccgtcgacg
     2341 tcgacgaacg cccggttgcc catcggcttg cccgccccgt acaggtggac ggcgacgccc
     2401 tgctgcgcgt cgggatcgcc gttgagcacc atcgtgcgca ggccgtcgac gaagtccagc
     2461 ggctcgggcg cgagcggcac cgggtgccag cgcagcgggt cgggcgggat gtcgacgccg
     2521 tcgcgggcgc ccgtcttgag cagcgcgtgg gcgtgcggcg cgtatgcccc gctgcgcacc
     2581 gacggctggc ggcggtagag ccaggtgcgc tggttgccgt ggcgcggcgc cgtgaacgcg
     2641 gtacccgaca gcagctcggc gtacaggccg aacggcgcct tctgcgggct gttgcggccc
     2701 tgcggcagcg cgccgggcac ggcctcgctg ctgaagtggt tgccgaagcc gctgtggcgg
     2761 ccgtcgttgg gggagacggt gcccgtcatc agtttcacct ttgttgaatc gattcaccaa
     2821 tgctgaatca tgggcgtatc atagtccgcg tcgtccaacc ctgtccaatc gccctgccgc
     2881 cgtgaaagcg cccgcccagc cccgtcccgc ccgcgaatcc gactcgcgcg gcatccagag
     2941 catcgaggtc ggcggccgcc tgctgctggc cctcgcccat cacggccaac cgctggcgct
     3001 caaggacctg gccgccgcgg ccgacatgac ggccggcaag gcgcacccct acctggtgag
     3061 cttcatcaag ctgggcctgg tcgagcgcga gaccgagggc ggccgctatg gcctgggccc
     3121 cctcgcgctg cagatgggcc tgatcgggct gcagcagtac gacccggtgc gcctggccac
     3181 cgagcgcatc gacgagctcg cccgcgccac cggccacacg gtcgcgatcg ccgtctgggg
     3241 ccatcgcggc ccgacgctgg tgcgcatcgc cgaggcgccg tcgccggtgc atgtgtcgat
     3301 gcgccacggc acggtgatga gcatccccga caccgcgtcg ggccgcctgt tcgccgcctt
     3361 cgggccggcc gacgccgtgc gcgaggcgct ggccaacgag gccaggctcg cgggcgcggg
     3421 cgcggccacg cgtcccgcgc gggcgcgcgc gggcgggcgc ttcggcctgg gcgcggcgtt
     3481 cgaccgcgag atcgaccggg tgcgtgccga cggcatcgcc ggcatcgacg gcgtcgccct
     3541 gcccggcctc agcgcgctgg cggcgccggt gttcgacgcc cgcggccagc tcgtgctgag
     3601 cctgacggcg atcgggccca gcgccacctt cgacgcggcc gtcgacggcg cggtcgcgcg
     3661 gcggctgaag ccggcagcgg cgcagctgtc gacgcggctg ggctggaagc cgggcgcgcc
     3721 gccgggcggc gcgcgctgaa aggtcgaagg gccggcgagg cgctcgcgcg ccccggccgg
     3781 ccccggcggg tcggatcagt tgccggcgat caggcggtcg cggcgccgtc gttggccgcg
     3841 cgcgcggcgg cgttctgctg gatgatgcgg cgatccagca ggcggcgcat gttgttcagc
     3901 gacgtgatgt ggatctggat caggcccagc aggccgttgc ggtacagctg cgcgatctgc
     3961 gcgtcggaca gcttgccgat ggcttcctcg tcggccgtca tgaagccgtc gaccgacagc
     4021 tgctcgccgc tcgccagcgt cgcgtcgaag cgcatcggct tgagcagctt gagctccagc
     4081 agcgcggcgc aggcctggcg ggtgcgctcc aggtcggtct cgaggtcctg cacgaacttg
     4141 tgcacgccgt tgagcagctc ggtggcctcg ccgttctcgt tgaacagcgg caggccctcg
     4201 gtgcgcgaca tgccttccca ggtgttgtcg aacaccatgg cccagcggtc cgagccctcg
     4261 atgcgggcca tcgtgaacgg ataggcgcgc agcatcgccg gcacgtagct ggcgtcccac
     4321 ttgccgtcgg cggtggcgaa caggttctcg cccggcttca ggccgaacac cgccacgggg
     4381 gcgacggtgt ccttgccgag cgcgtccggc gcggcgcgca cgaacaggat cgggaaggtc
     4441 agcgacgcgt cggcgaactc gctcaccgac acgatgaagg acgtgaacgg cgcgagcctg
     4501 ggaatcgcat tcagttcatt cttcaggcgc aggtcgcggt gaaggacggt gtcgagcgca
     4561 acgggctctt tgtgcaggtt cgtgacgatc atggttgttc tccaggggaa gggttccggc
     4621 tcacgagcac cttgtcgact cgcttgccgt cgaggtcgac gacctcgaat cgccagtcgc
     4681 cccattcgac cacgtcggcc gtccggggca ggcgtcccag caacagcatg atcatgccgg
     4741 ccagggtgtt gtagcgcccg cggtcctcct cgggcaagtc cttgatctcg aggcgatcct
     4801 tgatctcggg caccgggatc aggccgtcca tcagccagct gccgtcctcg cggcgcaccg
     4861 cccacgcatc atcgccggat tccgggccga attcacccgt gatcgcctcc agcacgtcgc
     4921 gcagcgtgat cacgccctgc actgcgccgt attcgtcgac gacgaagacg agctgcgcca
     4981 cgtggctgcg gaagtgatcg agcagttcca tgccggacag cgtctcgggc acgaacacgg
     5041 gcggctcgag ctcctcggtc agcaccggca tgcggccttc ggccagcggc ttgagcaggc
     5101 cctgcgcggt gatgacgccg atgacgtcgt ccaggccgcc gcggcacacc gggtagcgcg
     5161 aatggccgtc ctcctcggcg gcgaccaggc gcagcacctc ggcgatcggc gcgttggcgt
     5221 cgagccaggt gatctccgcg cgcgggatca tcatcgagcc gatctggcgg tcgtccaggc
     5281 ggaacacgtt gcgcaccatc tggtgctcct gcgcctcgat gacgccggcg tccaggccct
     5341 cttccaggct ggccgcgatc tcctcttccg tcacctcgcg cctggcgttg ccgcggatgc
     5401 ccagcaggcc caggatgaac tcggtggtct tggacagcag caccaccagc ggccgcgtgg
     5461 ccatcgacag ccacagcatc ggcggcgcca gcaggctggc caccgtctcg ggaaacatct
     5521 ggccgatgcg cttgggcacc agctcgccga agatgatggt gatcaccgtg atgatcagca
     5581 ccacgagggc cgtggcgctc aggtgggccg cgcgctgcgc caggccgaac gaatgcacca
     5641 gccactcgcc cagcggctcc gagaacgcgg cgtcgccgac gatgccattg agcacgctga
     5701 tcgacgtgat gcccacctgc accgtcgaca ggaacttggt ggggtggtcg tgcaggtcga
     5761 tcgcggcctc ggcgccgtgc tcgccagatt ccagcatcac ctgcaggcgc gccttgcggc
     5821 tggccgtcaa cgccatctcc gacatcgcga acgcggcgtt cagcaggatc aggaagaaga
     5881 gtagggcgac gtccatcgag aggcgcaggc agcgggcagt tcggggttcc ttcccggagg
     5941 ccggcggaac gagggttgcc ggcccggcat cccaggggaa atacgggggc gcgacagggc
     6001 caaagctcgt cagcgtagca gaatcgggcc atggattatc tgataggcga cctccagggc
     6061 tgctgcgacc cgctggacca tctgctcgcc acgatcgact tctccccgtc gcgcgaccgc
     6121 atccacctgc tgggcgacct cgtcaaccgg ggcccgcagt cgctgcccgt gctgcagcgc
     6181 ctggccgccc tcggcgacgc cgcgcagtgc ctgctcggca accacgacct gcacctgctg
     6241 gccgtcgccc acggcgtgcg caaggcgagc aagggcgaca cgctcgaccc gatcctcgcg
     6301 cacgaggacc gcgacgccct gctcgactgg ctgcgccacc gccgcatggc cgccgaggcc
     6361 tgcggctggc tgctggtgca cgcgggcgtc atcccgcaat ggagcgcgac ggacacgctc
     6421 gcgcacgccg ccgagatcga ggcgctgctg cgctccgacg acatcctcca attcctgccc
     6481 accatgtatg gcaacgagcc gcgccgctgg gatccggcac tgcaaggccc cgaccgctgg
     6541 cgcttcatcg tcaacgtcct cacgcgcatg cgcctgtgca gcgccgaggg cgagctcgaa
     6601 ctgaagaagg aaacccccgg cgaagccgac ccgcgcatgc agcgctggtt ctcccacccg
     6661 aaccgcctga cccgcgacgt ccccatcgcc ttcggtcact ggtcgcagct gggcctggtc
     6721 gacaccccga cgctgctgtg catcgacacc ggctgcatct ggggcggcca gctcaccgcc
     6781 gtgcgcgtcg acggcggccg ccgcgaggtc atccaggtgc cctgccccca ggcccgcaag
     6841 ccgggctgaa gagcccggtc gctacaatcg cggcctcgat ccaggaagcg tggcagagtg
     6901 gtcgattgca acggtcttga aaaccgtcgg cccgcaaggg cccgtgagtt cgaatctcac
     6961 cgcttccgcc aga
//
LOCUS       DATCRO010000136         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_564128, whole genome shotgun sequence.
ACCESSION   DATCRO010000136 DATCRO010000000
VERSION     DATCRO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564128"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VIN75_03740"
     CDS             complement(<1..206)
                     /locus_tag="VIN75_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8973303.1"
                     /translation="MQYVSYDTDGAVCTITLNRPDKRNAVDGLVAAELREAFGRFEAE
                     DALRVAVLTGAGGHFCAGADLSAVG"
     gene            291..1097
                     /locus_tag="VIN75_03745"
     CDS             291..1097
                     /locus_tag="VIN75_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator"
                     /protein_id="HEY8973304.1"
                     /translation="MDKVCNVPVAVPSAPDLILDLLVADGGTLSAQALCRAGALMGIG
                     ESTLRVGLTRLTAEGKIARGERGSYMLDRKGAALSRTVDAWHSRQAQTVAWKGDWLAV
                     HDAGVPRADKTAWRHHALALSLRGFAALHPGLHVRPDNLSGGLAAERKQLAALGLSSD
                     ALVFRIADLDGAAQAAARKLWNVRAMEADYRRLQAGVERSGKRLATQKLETATRESLL
                     LGRAVIGHLIRDPLLPAELMSPKPRAALLAATRRYQDKALVLWRKWLAQP"
     gene            complement(1111..>2451)
                     /locus_tag="VIN75_03750"
     CDS             complement(1111..>2451)
                     /locus_tag="VIN75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8973305.1"
                     /translation="VYGPAIRASDIDAPVVLGQGVEQVPGAIAFSSLLQSSEGPEVMR
                     AFEAIQPHDHAKYLLTSGSTGHPKVVVNTHRMLCANQQMIAQAWPFVERHKPVLLDWL
                     PWSHTFGANHNFNLVLRNGGTLYVDEGRPAPGLIEKTVRNLRETQPTLHFNVPRGLDM
                     LLPFLEADEALAADVLSRLRLAFYAAAALAPSTWKRLEAVARRVRPERPLWLTTSWGA
                     TETSPAITSAHWKLDGAGCIGAPLPGLELKFVPNGEKLEMRVKGVSVFPGYRDAPRET
                     AQAFDEEGYYRIGDAGFLAEPSQPAQGVIFNGRVAEDFKLSTGTWVSVGTLRVKLVSM
                     LAPHVQDIVLTGHDRDEIGMLVFASPQGAALAPQALGERIAAVMRSLRDEGAGSSQCP
                     ACALVLSEPPSLDAGEITDKGYINQRAVLARRAAEVERLHARSPGDVEVVRPKN"
BASE COUNT          356 a          870 c          876 g          349 t
ORIGIN      
        1 cccacggcgg agaggtcggc gcccgcgcag aagtggccgc cggcgccggt cagcaccgcg
       61 acgcgcaacg catcttcggc ctcgaagcgg ccgaaggcct cccgaagttc ggccgccacc
      121 aggccgtcga ccgcattgcg cttgtcgggc cggttcaggg tgatggtgca gacggcgcca
      181 tctgtgtcgt agctcacgta ctgcatggcc atgcgcgcat ccttccgggg caaaattaca
      241 tgttcgatcg ctcacgatga tcgtaggaaa tatttctctc gcccgcaaac atggacaaag
      301 tatgtaatgt gccggtcgcc gtacccagcg cgcccgacct gatcctcgac ctgctggtgg
      361 ccgacggcgg cacgctgagc gcccaggcgc tgtgccgcgc gggcgcactc atgggcatcg
      421 gcgaatcgac gctgcgcgtg ggcctgaccc ggctcacggc cgagggcaag atcgcgcgcg
      481 gcgagcgcgg cagctacatg ctcgaccgca agggcgcggc gctgtcgcgc accgtcgacg
      541 cctggcacag caggcaggcg cagaccgtgg catggaaggg cgactggctc gcggtgcacg
      601 acgccggcgt gccgcgtgcc gacaagaccg cgtggcgcca ccatgcgctc gcgctgtcgc
      661 tgcgagggtt cgccgcgctg cacccgggcc tgcacgtgcg gcccgacaac ctctcgggcg
      721 gtctcgcggc cgagcgcaag cagctggcgg cgctcggcct gtcttccgac gcgctggtgt
      781 tccgcatcgc cgacctcgac ggcgcggcgc aggcagcggc gcgcaagctc tggaacgtgc
      841 gcgccatgga ggccgactac cgtcgcctgc aggccggcgt ggagcgcagc ggaaagcggc
      901 tcgccaccca gaagctggag accgcgacgc gcgagtcgct gctgctgggc cgcgccgtga
      961 tcggccacct catccgcgat ccgctgctgc cggccgagct catgtcgccc aagccgcgcg
     1021 ccgcgctgct cgccgccacc cggcgctacc aggacaaggc gctggtgctg tggcgcaagt
     1081 ggctggcgca gccgtagctg cgcgcgggct tcagtttttc ggccgcacca cctctacgtc
     1141 gcccggcgag cgcgcgtgca gccgctcgac ctccgccgcg cgccgcgcga gcacggcgcg
     1201 ctggttgatg tagcccttgt cggtgatctc gcctgcgtcg aggctcggcg gctccgacag
     1261 caccagcgcg caggcggggc actgcgaaga gcccgcgccc tcgtcgcgca gcgatcgcat
     1321 cacggcggcg atgcgctcgc ccagtgcctg cggtgcaagc gcggcgccct gggggctcgc
     1381 gaagaccagc atgccgatct cgtcgcggtc gtggccggtg agcacgatgt cctgcacgtg
     1441 cggcgcgagc atcgacacca gcttcacgcg cagcgtgccc accgacaccc aggtgccggt
     1501 ggagagcttg aagtcttccg ccacgcggcc gttgaagatc acgccctgcg cgggctgcga
     1561 aggctcggcc aggaagccgg cgtcgccgat gcggtagtag ccctcttcgt cgaaggcctg
     1621 cgccgtttcg cgcggtgcgt cgcggtagcc gggaaacacg gacacgccct tgacgcgcat
     1681 ctccagcttc tcgccgttgg gcacgaactt cagctccagg cccggcagcg gcgcgccgat
     1741 gcagccggcg ccgtcgagct tccagtgcgc ggaggtgatg gcgggcgatg tctccgtcgc
     1801 gccccacgag gtggtgagcc acagaggccg ctcggggcgc acgcgccgcg ccaccgcctc
     1861 gagccgcttc caggtggagg gcgcgagcgc cgccgccgcg tagaaggcca ggcgcaggcg
     1921 cgagagcacg tcggcggcca gcgcctcgtc ggcctccagg aagggcagca gcatgtccag
     1981 cccgcgcggc acgttgaaat gcagcgtggg ctgggtctcg cgcaggttgc gcaccgtctt
     2041 ctcgatcagg cccggcgcgg gccggccttc atcgacgtac agcgtgccgc cattgcgcag
     2101 caccaggttg aagttgtggt tggcgccgaa ggtgtggctc cagggcagcc agtcgagcag
     2161 caccggcttg tggcgctcca cgaagggcca ggcctgggcg atcatctgct ggttggcgca
     2221 cagcatgcgg tgggtgttga ccaccacctt gggatggccg gtggagcccg aggtcagcag
     2281 gtacttggca tggtcgtggg gctggatggc ctcgaacgcg cgcatcacct ccggcccttc
     2341 ggaggactgc aacagcgacg agaacgcgat ggcgcccggc acctgctcca cgccctgccc
     2401 cagcaccacc ggcgcatcga tgtcactggc gcggatggcc gggccgtaca c
//
LOCUS       DATCRO010000137        11393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_822834, whole genome shotgun sequence.
ACCESSION   DATCRO010000137 DATCRO010000000
VERSION     DATCRO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822834"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIN75_03755"
     CDS             complement(<1..186)
                     /locus_tag="VIN75_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8973306.1"
                     /translation="MTLQDPCRAEVPGLHVLVLPGWQNSGPRHWQSRWEAVYGDTRVE
                     QSDWMHPLRGDWMMRLEE"
     gene            288..1277
                     /gene="argC"
                     /locus_tag="VIN75_03760"
     CDS             288..1277
                     /gene="argC"
                     /locus_tag="VIN75_03760"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY8973307.1"
                     /translation="MSTAKKFRVYVDGQEGTTGLRIHEMLAARADLELLRISAELRKD
                     DAERARLLNAADVAFLCLPDEASRAAAKMVTNPDTCLIDASTAHRTDPTWVYGLPELA
                     PGQREALRTAKRIANPGCHATAFILLMRPLVDAGLVAPDANVSATSITGYSGGGKKMI
                     EQYQAWHARQMDGERQIDAALSAPRPYALGLAHKHIPEMKTCSRLTRAPVFMPIVGNF
                     YKGLTVSIPFHVGQLAAGTDAAKVHATLARRYADEQFVCVMPLADPAVLAEGSFDVQA
                     CNDTQRADLFVFGHADQVLLMARIDNLGKGASGAAVQTMNLHLGLPEGESLDI"
     gene            1354..2745
                     /locus_tag="VIN75_03765"
     CDS             1354..2745
                     /locus_tag="VIN75_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8973308.1"
                     /translation="MTNASAETLRDSRGLRLGTTSRAAADRADEALWQMMTFADTPRL
                     ALQAAREADPGWALPLLLDAGFRLGLNQPDDREAVRELLASAGALASRANARERAHLQ
                     ALERLQDGRVADALRIWDDLLLEQPRDALAMHWAHQWDHARGDSASMRLRPARALPEW
                     DDADPLFPCVLGLYAFGLAECHQHGLAEDLGRRAIALAQQQSADPATPARVPWAVHAV
                     THAMEMQGRFDDGAIWLRQQQAGWTDGTRFASHLWWHLALFRLEALDVTGALRLVDTH
                     LSGSALTHGLQCMDAAALYWRLRVMDVEVDALFKDLLRRWTPDPGQAGASAFTDWHVV
                     LALLGAGERAQAEAWVARCAERVMRAEEGARSNHAVARDLGLPLMRALLASERGDHDA
                     AVRGLYLVHESAARLGGSHVRRDLIAQTLMHSAAASGVPHIGRAVLNERCLAKPVTPL
                     TRHWAGKLGVAIA"
     gene            complement(2760..3629)
                     /locus_tag="VIN75_03770"
     CDS             complement(2760..3629)
                     /locus_tag="VIN75_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8973309.1"
                     /translation="MPLTHLLLALAVVAIWGTNFVVIRLGLDTFAPYTFAALRYTFSV
                     LPFVLFIRRPAVSWGKLAAFGVLIGVGQFGLLFWAMRSDISPGLASLVIQSQVFFTIG
                     LAAALYGERLKRLQWPAFALAIAGLAIVATHVSAGTGVTLFGVALVTAGAISWAGGNL
                     VARAAGRVDALGFMVWSSPFAAVPLWAITLVVDGPAAVGGALAHAGAAAWAAVAWQAI
                     GNTLFGYGVWNWLLARHPASTVTPTALLVPVFGMSASAIALHEGLPAWKLGAAALVMG
                     GLALNVLATRRRA"
     gene            complement(3808..3951)
                     /locus_tag="VIN75_03775"
     CDS             complement(3808..3951)
                     /locus_tag="VIN75_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973310.1"
                     /translation="MTIALASLLRRLATRAARGLCTGSGRFAVIVGIAVAIASSAHAG
                     PAP"
     gene            complement(3999..4202)
                     /locus_tag="VIN75_03780"
     CDS             complement(3999..4202)
                     /locus_tag="VIN75_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8973311.1"
                     /translation="MRNRMRELRTERGWSQTELGERLGVSRQTVNAIENERYDPSLPL
                     AFQIARAFGLTIEQIFIEDGRAA"
     gene            complement(4199..4591)
                     /locus_tag="VIN75_03785"
     CDS             complement(4199..4591)
                     /locus_tag="VIN75_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973312.1"
                     /translation="MDVPRMTNRQYLKELTATLLVYGAMLLGSIELLTHVEVARPWRD
                     LVALSPMLPAAATAWVILRELRRMDELQRRIQLEALGFSFAGTAILTFSYGFLEGLGY
                     PKLSMFTVWPILAVLWVVGLVLARRRYQ"
     gene            complement(4664..5374)
                     /locus_tag="VIN75_03790"
     CDS             complement(4664..5374)
                     /locus_tag="VIN75_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEY8973313.1"
                     /translation="MRRADRLFRIVQLLRGRRLSTAAWIAEKLEVSVRTVYRDVADLQ
                     GQGVPIDGEAGVGYRLAGGFDLPPLMFTTLEAQALVASVRLAQSRLDPALALAAEDAL
                     AKIVGVLPPVARAAAESLPLFAAPRARDHESAERLGLLRDAASARRRIRFDYADAQGA
                     ATSRTARPLGCYHWESVWTLAAWCEHRDAFRSFRVDRMRGLDVLDDVFRDEPGRTLPD
                     FLRHVQESRKAYEATKML"
     gene            complement(5402..5776)
                     /locus_tag="VIN75_03795"
     CDS             complement(5402..5776)
                     /locus_tag="VIN75_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8973314.1"
                     /translation="MSHNAIHWFEIPVADLDRAQRFYETLFARSMRREQMGPQELAVF
                     AYEDGIGGALIKSATAPKPSLEGVLVYLNAGPSLDVVLSRANELGAQVLMPKLELPRD
                     IGFIAQIVDCEGNRIGLHSPAA"
     gene            complement(5924..7207)
                     /locus_tag="VIN75_03800"
     CDS             complement(5924..7207)
                     /locus_tag="VIN75_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8973315.1"
                     /translation="MKLIRRVSAVMAAAAIGLGSTAALAGSLSISVSADDNAQREAYK
                     TLVADFHTANPDVDVHLTIQDAPTYRKALPGTLDSDNAPDVFNWFAGDQLRMMAQRGQ
                     LDDVSDVWKANTWWNTFPSAAVTVAGKQYALPYQYYPWGLFTRRDVLAAAGIREQPHD
                     LSAIMTACSKLRKAGFTPIALGAKDGWALAAWFDFIDMRANGYEYHQQLLDGKATYND
                     ANVRRTFAMWKQLIDAKCFDPNALAVDERGAKGEFYGGHAGMLLMGTIVSASFPEVVR
                     PVIDYQKFPTIDAGQAAAEAAPTDTFQIAARAKNKADARRFLKFVSSSTTSAKLAKAI
                     GSFPTNKFAPVAGTVLDLASYKVLTDAKTNIVQGYDRDVPADMAAAGIKGFQEFFAKP
                     DQMYAVMNRLDATRGPAYAALADQQAAPAAKGRKN"
     gene            complement(7378..8142)
                     /locus_tag="VIN75_03805"
     CDS             complement(7378..8142)
                     /locus_tag="VIN75_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973316.1"
                     /translation="MNSDSRPSRVLLVTGASRGIGAACALRAAEAGWDVGVNFLRDQD
                     AAERVAAQVRERGRRACTLRADVASEGAVEAMFKALDAELGPLGGLVNNAGIVDVAAR
                     VDEMPAARVARLFNVNVVGAFTCAREAVRRMSTRHGGHGGVVVNLSSAAARLGSAGVY
                     VDYAATKAAIDCLTLGLAREVAGEGIRVNGVRPGIIDTEIHATSGMLDRTRSAVDQIP
                     LQRLGTADEVAQAVVFLLSDAAGYVTGATLDVAGGR"
     gene            complement(8177..9694)
                     /locus_tag="VIN75_03810"
     CDS             complement(8177..9694)
                     /locus_tag="VIN75_03810"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8973317.1"
                     /translation="MPTPAFSSSTPVGHFIGGEHVVHADARKQSVFNPATGAVARQLH
                     LGTAADVDAAAAAASKAFEGWSNTPAIKRARVLNAFLALLNEHRDQLAAMITAEHGKV
                     FSDAQGEVMRGIDVVEFACGIPQLLKGDYTEQVSTGIDNWTMRQPLGVVAGITPFNFP
                     FMVPCWMFPVALACGNTFILKPSERDPSPSLFIAELLQRAGLPDGVFNVVQGDKTVVD
                     ALLVHPDVKGLSFVGSTPIAQYIYETGAHHGKRVQALGGAKNHMVVMPDADVEQSIDA
                     LVGAAYGSAGERCMAISVAVLVGDVADRLLPGLAERARKLVIDNGMNPAAEMGPVITK
                     EALQRIEGYIDIGVKEGATLLVDGRGHQVSGHENGFFTGGTLFDDVTPQMRIYKEEIF
                     GPVLACVRVPDFRSAVDLVNAHEFGNGVACFTSDGNVAREFARRIQVGMVGINVPIPV
                     PMAWHGFGGWKKSLFGDMHAYGEEGVRFYTKQKSVMQRWPASIGKGAEFAMPTAR"
     gene            9876..10850
                     /locus_tag="VIN75_03815"
     CDS             9876..10850
                     /locus_tag="VIN75_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8973318.1"
                     /translation="MDARKIESLWSHVHGLGVLQSAGSFTAAAQRLGLSKAAMSQRVA
                     ELERAAGVPLVRRTTRSVRLTEAGERLVEATRGAFEQIEQGFAGVQDLVGEPHGVLRV
                     SAPVALGRQQIVPRLAGFLRAYPQVRVELELSDRLVAMAQEGFDVALRHTAAPPDTHV
                     AWPLCGTRSWLVASRAYLRRAGAPAAPADLATHDCLHYLRPGDTPTWSFEPDAARGAR
                     AGGRVSVAVRGVFAANNSEALREAALAGLGIALLPDFSAQAAVQAGKLQLVLPHWKPV
                     GAFGEHLYAIRPYSPVVPRAVQAFIGYWRTALEDGFPLSPPAQAPRAR"
     gene            10889..11284
                     /locus_tag="VIN75_03820"
     CDS             10889..11284
                     /locus_tag="VIN75_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973319.1"
                     /translation="MRTSDLRVLAESANFLLSHAAESVYVVDKRDGSTRVAGEHVGDP
                     GVGLITADEQWFCSGGEGVQCLSRAGVQLSFFRGRASADASAQTSVWFVHGMSEIAPG
                     QLRVVFDHHEHGIWMIDLATARRWQEEPR"
BASE COUNT         1641 a         4044 c         4087 g         1621 t
ORIGIN      
        1 ctcctcgagc cgcatcatcc agtcgccgcg cagcgggtgc atccagtcgc tctgctccac
       61 gcgcgtgtcg ccgtagaccg cctcccagcg gctctgccaa tgccgcggcc cggagttctg
      121 ccagccgggc agcaccagca cgtgcagtcc cgggacttca gcgcgacagg gatcctgtaa
      181 tgtcattgcc ttatgcttca cgtctgaaac ggcgcgccgg agctgcgcgc tacgcaacga
      241 ttctgccgtg cggcccgagc gcgcgcggcg cccggagatc ctgaaaaatg agcaccgcca
      301 agaaattccg cgtctacgtc gacggccagg aaggcaccac cggcctgcgc atccacgaga
      361 tgctggccgc ccgcgccgac ctggagctgc tgcgcatctc cgccgagctg cgcaaggacg
      421 acgccgagcg cgcgcgcctg ctcaatgccg ccgacgtcgc cttcctgtgc ctgcccgacg
      481 aggcttcgcg cgccgcagcg aagatggtga ccaacccgga cacgtgcctg atcgacgcca
      541 gcaccgcgca ccgaacggat ccgacctggg tctacggcct gcccgagctc gcgcccggcc
      601 agcgcgaggc gctgcgcacg gccaagcgca tcgccaatcc gggctgccac gccaccgcgt
      661 tcatcctgct gatgcgcccg ctggtcgacg ccgggctcgt cgcgcccgac gccaatgtca
      721 gcgcgacgtc gatcaccggg tattccggcg gcggcaagaa gatgatcgag cagtaccagg
      781 cctggcacgc gcgccagatg gacggcgagc gccagatcga cgccgcgctc agcgcgccgc
      841 gcccgtacgc gctgggcctc gctcacaagc acatccccga gatgaagacg tgctcgcggc
      901 tcacgcgcgc gccggtattc atgcccatcg tcggcaactt ctacaagggc ctgacggtca
      961 gcatcccgtt ccatgtcggc cagttggccg ccggcaccga cgccgccaag gtgcacgcca
     1021 cgctcgccag gcgctatgcc gacgaacagt tcgtgtgcgt gatgccgctg gccgaccccg
     1081 cggtgctggc cgagggctcg ttcgacgtcc aggcctgcaa cgacacgcag cgcgccgacc
     1141 tgttcgtgtt cggccatgcc gaccaggtgc tgctgatggc gcgcatcgac aacctcggca
     1201 agggcgcgtc cggcgcggcg gtgcagacga tgaacctgca cctgggcctg cccgagggcg
     1261 agagcctcga catctgaaca tgcgcatgcc acgcgccgtc ccatcctcac gcgcaccagc
     1321 gttgcggcgc gccgtccgcg gagcgctcgt cgcatgacga acgcgtctgc cgagaccttg
     1381 cgggactcgc gcggactgcg tctgggaacc acgtcgcgcg cggccgccga ccgcgccgac
     1441 gaggcgctgt ggcagatgat gaccttcgcc gacacgccgc gcctggcgtt gcaggcggcg
     1501 cgcgaggccg atcccggctg ggcgctgccg ctgctgctgg acgcgggctt tcgcctcggc
     1561 ctgaaccagc ccgacgaccg cgaagccgtg cgcgagctgc tcgcgtccgc gggcgcgctg
     1621 gcgtcccgcg cgaacgcgcg cgagcgggcg cacctgcagg cgctcgaacg cctgcaggac
     1681 ggccgcgtgg cggatgcgct gcgcatctgg gacgacctgc tgctggaaca gccgcgcgac
     1741 gctctggcca tgcactgggc ccaccagtgg gaccacgccc gcggcgattc ggccagcatg
     1801 cgcctgcgcc cggcgcgcgc gctgccggag tgggacgacg ccgacccgct gttcccctgc
     1861 gtgctgggcc tgtatgcgtt cggcctggcc gagtgccatc agcatggcct ggccgaggac
     1921 ctgggccggc gcgcgatcgc gctggcgcag cagcagtcgg ccgacccggc gacgccggcg
     1981 cgggtgccct gggcggtgca cgccgtcacg cacgcgatgg agatgcaggg ccgcttcgac
     2041 gacggcgcca tctggctgcg ccagcagcag gccggctgga ccgacggaac gcgcttcgcg
     2101 agccatctgt ggtggcatct cgcgctgttc cgcctcgagg cgctggacgt caccggcgcg
     2161 ctgcggctgg tcgacacgca cctgtccggc agcgcgctca cgcacggact gcaatgcatg
     2221 gacgccgccg cgctgtactg gcggctgcgc gtgatggacg tcgaggtcga cgccctcttc
     2281 aaggacctgc tgcgccgctg gacgcccgac cccgggcagg ccggcgcgag cgcgttcacc
     2341 gactggcacg tggtgctcgc gctgctgggc gcaggcgagc gcgcgcaggc cgaggcctgg
     2401 gtggcgcgct gcgccgagcg cgtgatgcgc gccgaggagg gcgcgcgcag caaccacgcg
     2461 gtcgcgcgcg acctcgggct gccgttgatg cgtgcgctgc tcgcctccga gcgcggcgac
     2521 cacgacgccg cggtgcgcgg gctgtacctg gtgcacgaat cggccgcgcg gctgggcggc
     2581 agccatgtcc ggcgcgacct gatcgcgcag accttgatgc acagcgcggc cgccagcggc
     2641 gtgccgcaca tcgggcgtgc ggtgctcaac gagcgctgcc tggccaagcc ggtgacgccg
     2701 ctcacgcgcc actgggccgg caagctgggc gtcgcgatcg cctgaagcgc cggcgtgcgt
     2761 caggcgcgcc ggcgcgtcgc cagcacgttc agcgcgagcc cgcccatcac gagtgccgcc
     2821 gcgccgagct tccaggccgg cagcccttcg tgcagcgcga tcgccgaggc gctcatgccg
     2881 aacacgggca ccagcagcgc ggtgggcgtg acggtggacg ccgggtggcg cgcgagcagc
     2941 cagttccaca cgccatagcc gaacagggtg ttgccgatgg cctgccacgc gaccgcggcc
     3001 caggccgccg cgcccgcgtg tgccagcgcg ccgcccaccg cggccgggcc gtccacgacc
     3061 agcgtgatcg cccacagcgg cacggccgcg aacgggctcg accacaccat gaagccgagc
     3121 gcgtccacgc gccccgccgc gcgcgcgacg aggttgccgc cggcccagct gatggccccg
     3181 gccgtcacga gcgcgacgcc gaacagcgtc acgccggtgc ccgccgacac gtgcgtggcc
     3241 acgatcgcca gccccgcgat cgccagcgcg aacgccggcc actgcagccg cttgagccgc
     3301 tcgccgtaca gcgccgcggc cagcccgatc gtgaagaaca cctgcgactg gatcaccagc
     3361 gacgcgaggc cgggcgagat gtccgagcgc atcgcccaga acagcagccc gaactgcccc
     3421 acgccgatca gcaccccgaa cgccgcgagc ttgccccacg acaccgccgg cctgcggatg
     3481 aacagcacga acggcagcac cgagaacgtg tagcgcagcg ccgcgaaggt gtagggcgcg
     3541 aaggtgtcga gcccgaggcg gatgacgacg aagttggtgc cccagatcgc gacgacggcc
     3601 agggcgagca gcaggtgggt caggggcatc gccgcagtct aggcgagcga cgctgtcctt
     3661 cgggcgcgtc cgtccattcc tgccgccgcc aaccacgcgt ccggatatgg cgtgccgcgg
     3721 tggacgcgac ggcgtcgaat cgcgtcctca cccaaccatg gagaacccga tgacccggct
     3781 tgccgtccgc ggcctttcca ccggaaccta gggcgcgggc ccggcatgcg cggacgaggc
     3841 gatcgcgacg gcgatgccga cgatgaccgc gaagcggccc gagccggtgc acaggccgcg
     3901 cgcggcccgg gtggcgagtc ggcgcagcaa cgaagcgaga gcgatggtca tggggtctcc
     3961 ttggaagcat ggggcacgca gggccggttg cgcagtggct aagccgccct gccgtcctcg
     4021 atgaagatct gctcgatcgt caggccgaag gcccgggcga tctggaaggc gagcggcagg
     4081 ctggggtcgt agcgctcgtt ctcgatcgcg ttgacggtct gccgggacac gcccaggcgc
     4141 tcgccgagtt cggtctggga ccagccgcgc tcggtgcgca gttcgcgcat gcggttcctc
     4201 attggtagcg cctgcgagcc agcacgaggc cgaccaccca cagcacggcc aggatcggcc
     4261 atacggtgaa catcgacagc ttcgggtagc ccaggccctc gaggaagccg taactgaacg
     4321 tgaggatggc ggtgccggcg aacgagaagc cgagcgcctc cagctggatg cggcgctgca
     4381 gctcgtccat gcggcgcagc tcgcgcagga tcacccaggc cgtcgcggcc gcgggcagca
     4441 tcgggctgag cgccacgagg tcgcgccacg gccgggcgac ctcgacgtgc gtgaggagct
     4501 cgatcgagcc gagcagcatc gcgccgtaga ccagcagcgt cgccgtcagt tccttcaggt
     4561 attgccggtt cgtcattcgc ggaacgtcca tgtggaaagg atgctttcca ttgtggggac
     4621 gttccggggc gctgtaaagg cccctttaca tggaaagggt cttttacagc atcttggtgg
     4681 cttcgtacgc cttgcgcgac tcctgcacgt gccgcaggaa gtcgggcaac gtgcggcccg
     4741 gctcgtcgcg gaagacgtcg tcgagcacgt ccagcccgcg catgcggtcg acgcggaagc
     4801 tgcggaacgc gtcgcggtgc tcgcaccagg cggccagcgt ccagaccgat tcccagtggt
     4861 agcagccgag cggccgcgcg gttcgcgacg tggccgcgcc ctgcgcgtcc gcgtagtcga
     4921 agcggatgcg ccggcgcgcg gacgccgcgt cccgcaacag gcccaggcgc tcggcgctct
     4981 catggtcgcg cgcccgcggc gcggcgaaca gcggcagcga ctcggccgcg gcccgagcga
     5041 cgggcggcag cacgccgacg atcttggcca gcgcgtcctc tgcggccagc gccagcgccg
     5101 ggtcgagccg gctctgggcc agccgcaccg atgcgaccag cgcctgggcc tcgagcgtgg
     5161 tgaacatcag cggaggcagg tcgaagccgc ccgcgagccg gtagccgacg cccgcctcgc
     5221 cgtcgatcgg cacgccctgc ccctgcaggt cggccacgtc gcggtagacg gtgcgcacgg
     5281 agacttccag cttctccgcg atccaggcag cggtggacag ccgccgcccg cgcagcagct
     5341 ggacgatccg gaagagacga tcggcacggc gcatgactcg cagtgtagga gcgtcgcggc
     5401 ttcaggcggc aggcgagtgc aggccgatgc ggttgccctc gcagtcgacg atctgcgcga
     5461 tgaagccgat gtcgcgcggc agttccagct tgggcatcag cacctgggcg ccgagttcgt
     5521 tcgcgcgcga cagcacgacg tccagcgacg gcccggcgtt caggtagacg agcacgcctt
     5581 cgaggctggg cttgggcgcg gtcgcgctct tgatcagcgc gccgccgatg ccgtcctcgt
     5641 acgcgaacac ggccagctcc tgcgggccca tctgctcgcg ccgcatcgag cgcgcgaaca
     5701 gggtctcgta gaagcgctgc gcgcgatcga ggtcggcgac ggggatctcg aaccagtgga
     5761 tggcgttgtg cgacatggag ggcctcggtg gggttggttg gaagaggact ccatgctgct
     5821 cgggggctgc tgacaccgtc ctgtcagcag ctttgccagc atcaaaagaa aaccggcgcg
     5881 ccccctcgga cgcgccggct tcatcgggga ctggccgcgg cgatcagttc ttgcgaccct
     5941 tggccgccgg cgcggcctgc tggtcggcca gcgcggcgta ggcggggccg cgcgtcgcgt
     6001 cgaggcggtt catgacggcg tacatctggt cgggcttggc gaagaactcc tggaagccct
     6061 tgatgccggc ggcggccatg tcggcgggga cgtcgcggtc gtagccctgg acgatgttgg
     6121 tcttggcgtc ggtcagcacc ttgtacgacg cgaggtcgag cacggtgccg gccaccggcg
     6181 cgaacttgtt cgtcgggaac gagccgatcg ccttggccag cttggcgctg gtggtgctgc
     6241 tgctgacgaa cttcaggaag cggcgcgcgt cggccttgtt cttggcgcgg gccgcgatct
     6301 ggaaggtgtc ggtcggcgcg gcctcggcgg cggcctggcc ggcgtcgatg gtcgggaact
     6361 tctggtagtc gatcaccggg cgcacgacct ccgggaagct ggcgctgacg atggtgccca
     6421 tcagcagcat gccggcgtgg ccgccgtaga actcgccctt ggcgccgcgc tcgtcgacgg
     6481 ccagcgcgtt ggggtcgaag cacttggcgt cgatcagctg cttccacatc gcgaaggtgc
     6541 ggcgcacgtt ggcgtcgttg taggtggcct tgccgtccag cagctgctgg tggtactcgt
     6601 agccgttggc gcgcatgtcg atgaagtcga accacgcggc cagcgcccag ccgtccttgg
     6661 caccgagcgc gatcggcgtg aagccggcct tgcgcagctt ggagcacgcg gtcatgatcg
     6721 cgctgaggtc gtgcggctgc tcgcggatgc cggcggcggc gagcacgtcg cggcgcgtga
     6781 acaggcccca tgggtagtac tggtacggca gcgcgtactg cttgccggcg acggtgacgg
     6841 ccgcgctggg gaacgtgttc caccaggtgt tggccttcca gacgtcgctg acgtcgtcga
     6901 gctggccgcg ctgcgccatc atgcgcagct ggtcgccggc gaaccagttg aagacgtcgg
     6961 gcgcgttgtc cgagtccagc gtgccgggca gcgccttgcg gtacgtgggc gcgtcctgga
     7021 tcgtcaggtg cacgtcgacg tcggggttgg ccgtgtggaa atcggccacg agcgtcttgt
     7081 aggcctcgcg ctgcgcgttg tcgtcggcgc tcacgctgat gctcagagag ccggccagcg
     7141 ccgcggtcga acccaggccg atggccgctg cggccatcac ggccgagaca cgtcgaatca
     7201 acttcatggt cttttccctt cacacccacc gacggaacga ttatgtaagg gatgaaaccg
     7261 cttgcccgct aggggctgac actatgtcag cggggcgcca cgtgacgcat ctcacgcgga
     7321 cctgtgcaga aagtgccgcg aacgcgtcac aaacggcccg atgggccgcg ccgggattca
     7381 gcgtccgccg gcgacgtcga gcgtcgcgcc ggtgacgtag cccgccgcgt ccgacagcag
     7441 gaagaccacg gcctgcgcga cctcgtcggc ggtgcccagg cgctgcagcg ggatctggtc
     7501 gaccgccgac cgcgtgcgat cgagcatgcc gctggtggcg tggatctcgg tatcgatgat
     7561 gccgggccgc acgccgttga cgcggatgcc ttcgccggcc acctcgcgcg ccaggcccag
     7621 cgtgaggcag tcgatcgcgg ccttggtggc ggcgtagtcg acgtagacgc cggccgagcc
     7681 caggcgcgcc gcggccgacg acaggttgac caccacgccg ccgtgtccgc catggcgcgt
     7741 gctcatgcgc cgcacggcct cgcgggcgca ggtgaaggcg cccaccacgt tgacgttgaa
     7801 caggcgcgcg acgcgcgcgg ccggcatctc gtcgacgcgc gcggccacgt cgacgatgcc
     7861 cgcgttgttg acgagcccgc cgagcggccc gagctcggcg tcgagcgcct tgaacatcgc
     7921 ctcgaccgcg ccttcgctcg cgacgtccgc acgcagcgtg cacgcgcgcc gcccccgctc
     7981 gcgcacctgc gcggcgacgc gctcggcggc gtcctggtcg cgcaggaagt tgacgccgac
     8041 gtcccagccg gcctcggccg cgcgcagggc gcaggcggcg ccgatgccgc ggctggcgcc
     8101 ggtgaccagc aggacgcggg acggtcggga gtccgagttc atgccgcgtt tctaacacca
     8161 cggccgtcgc gagggcctac ctggccgtcg gcatcgcgaa ttcggcgccc ttgccgatgg
     8221 aggccggcca gcgctgcatc acgctctttt gcttcgtgta gaagcgcacg ccctcctcgc
     8281 cgtacgcgtg catgtcgccg aacaggctct tcttccagcc gccgaagccg tgccacgcca
     8341 tcggcaccgg aatcggcacg ttgatgccca ccatgcccac ctggatccgg cgcgcgaact
     8401 cgcgggcgac gttgccgtcg ctggtgaagc aggccacgcc gttgccgaac tcgtgcgcgt
     8461 tgacgaggtc gaccgccgac ctgaagtccg gcacgcgcac gcaagccagc acggggccga
     8521 agatctcttc cttgtagatg cgcatctgcg gcgtgacgtc gtcgaacagc gtgccacccg
     8581 tgaagaagcc gttctcgtgg cccgacacct ggtggccgcg gccgtccacc agcagggtcg
     8641 cgccttcctt gacgccgatg tcgatgtagc cctcaatgcg ctgcagcgct tccttggtga
     8701 tgaccgggcc catctcggcg gccgggttca tgccgttgtc gatgacgagc ttcctcgcgc
     8761 gctcggccag cccgggcagc aggcggtcgg cgacgtcgcc caccagcacg gccaccgaga
     8821 tcgccatgca gcgctccccg gccgagccgt atgccgcgcc gaccagcgcg tcgatggact
     8881 gctcgacgtc ggcgtcgggc atcacgacca tgtggttctt cgcgccgccc agcgcctgca
     8941 cgcgcttgcc gtggtgcgcg ccggtctcgt agatgtactg cgcgatcggc gtcgagccga
     9001 cgaaggacag gcccttgacg tcgggatgca ccagcagcgc gtcgacgacg gtcttgtcgc
     9061 cctgcaccac gttgaagacg ccgtcgggca gccccgcgcg ctgcagcagc tccgcgatga
     9121 acaggctggg cgacgggtcg cgctcgctgg gcttgaggat gaaggtgttg ccgcaggcca
     9181 gcgccaccgg gaacatccag cacggcacca tgaacgggaa gttgaacggc gtgatgccgg
     9241 ccaccacgcc cagcggctgg cgcatcgtcc agttgtcgat gccggtggag acctgctcgg
     9301 tgtagtcgcc cttgagcagc tgcgggatgc cgcaggcgaa ctcgacgacg tcgatgccgc
     9361 gcatcacctc gccctgggcg tcggagaaca ccttgccgtg ctcggcggtg atcatggcgg
     9421 cgagctggtc gcggtgctcg ttgagcagcg cgaggaacgc gttgagcacg cgcgcccgct
     9481 tgatcgccgg cgtgttggac cagccctcga acgccttgct cgcggccgcg gccgcggcgt
     9541 cgacgtcggc cgccgtgccc aggtgcagct ggcgcgcgac ggcgcccgtg gccgggttga
     9601 agacggactg cttgcgcgca tccgcgtgca cgacgtgctc gccgccgatg aagtggccga
     9661 ccggagtcga ggacgagaag gcgggcgtgg gcatgagcgg tctcctggac gcggtggcat
     9721 ggcgacccgg gcggtcattc ctgcgcgcag tcgcaggatc ccccggggat gacgcagcga
     9781 gtctaggcat tcgccatccg cccgggaagc tcgcgccgct ggattcactg ttcatttgga
     9841 tgaacaatcg acgcatcgaa tttcggacaa acctcatgga cgcccgcaag atcgaatcgc
     9901 tctggtcgca cgtgcacggc ctgggcgtgc tccagagcgc cggcagcttc accgcggcgg
     9961 cgcagcgcct gggcctgagc aaggcggcaa tgagccagcg cgtggccgaa ctcgagcgcg
    10021 cggccggcgt gccgctggtg cgtcgcacga cgcgcagcgt tcgcctgacc gaggccggcg
    10081 agcggctggt cgaggccacg cgtggcgcct tcgagcagat cgagcagggc tttgccggcg
    10141 tgcaggacct cgtcggcgag ccgcacggcg tgctgcgcgt cagcgcgccc gtggcgctcg
    10201 ggcgccagca gatcgtgccg cgcctggccg gcttcctgcg cgcgtacccg caggtgcgcg
    10261 tggagctgga gctgtccgac cggctggtcg cgatggcgca ggagggcttc gacgtcgcgc
    10321 tgcgccacac cgctgcgccg cccgacaccc acgtcgcctg gccgctgtgc ggcacccgca
    10381 gctggctggt cgcctcgcgc gcgtacctgc gccgcgcggg cgcacccgcg gcgcccgccg
    10441 acctggccac gcacgactgc ctgcactacc tgcgccccgg cgacacgccc acctggagct
    10501 tcgagcccga cgccgcgcgc ggcgcgcggg ccggcggccg cgtcagcgtg gcggtgcgcg
    10561 gcgtcttcgc ggccaacaac agcgaggcgc tgcgcgaggc tgcgctcgcg ggcctgggca
    10621 tcgcgctgct gcccgacttc agcgcccagg ccgccgtcca ggccggcaag ctgcagctcg
    10681 tgctgccgca ctggaaaccg gtgggcgcgt tcggcgagca cctctatgcg atccggccgt
    10741 acagccccgt cgtaccccgc gcggtgcagg ccttcatcgg ctactggcgc acggcgctgg
    10801 aggacggctt tcccctgtcg ccgcccgcgc aggctccgcg cgcgcgctga cgcgcgtacg
    10861 atgcgagttc gtcgccaagg aggacgccgt gcgaacgagt gacctgcgcg tgctggccga
    10921 gtcggccaac ttcctgctga gccatgccgc ggagtcggtc tatgtggtcg acaagcgcga
    10981 cggctcgacc cgcgtcgcgg gcgagcacgt cggcgatccg ggcgtcgggc tcatcacggc
    11041 cgacgagcag tggttctgca gcggcggcga gggcgtgcaa tgcctgtcgc gggccggggt
    11101 gcagctcagc ttcttccgcg gacgcgcgtc ggcggacgcg tcggcgcaga cgtcggtgtg
    11161 gttcgtccac gggatgtccg agatcgcgcc ggggcagttg cgcgtcgtct tcgatcacca
    11221 tgagcacggg atctggatga tcgacctggc caccgcccgg cgttggcagg aggagccgcg
    11281 ctgacgggat ctccgaagcc ccgtagggcg ggttagcgca gcgtaacccg ccattcgtcg
    11341 gcgctcgcac cgtcctgcct tcggcgatcg acacgggacg tcgcgaatgg cgg
//
LOCUS       DATCRO010000138         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_327925, whole genome shotgun sequence.
ACCESSION   DATCRO010000138 DATCRO010000000
VERSION     DATCRO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327925"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /gene="ssb"
                     /locus_tag="VIN75_03825"
     CDS             <1..373
                     /gene="ssb"
                     /locus_tag="VIN75_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8973320.1"
                     /translation="TQWHRVTFYDRLAEIAGEYLKKGKPVYIEGRLKYGKYTDKDGIE
                     RNTVDIVAEQMQMLGGRDGGGGGDEGGGGSGGRYLAPRDGVDGNEDRNASRPAARPAP
                     APRPAPKASTGFDDMDDDIPF"
     gene            complement(522..2009)
                     /locus_tag="VIN75_03830"
     CDS             complement(522..2009)
                     /locus_tag="VIN75_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8973321.1"
                     /translation="MNLTDYLRCDAVALARLHRTQPRINAVCRLMDAEARAQLAAPLP
                     GGPFTGVPFLLKDGVQDVAGLPTGYGSAGMRHVVPTETAYVVRRYLAAGLVIFGKTNL
                     PEFALKGVTDSRANGRTNNPWNVEHVPGGSSGGAAAAIASGVLPMAAAADGGGSIRIP
                     AACCGLFGLRPSRGRVSSGPGIGEAWFGASSEGVISRSVRDTARMLDALAGAEPGDPV
                     VIAPPDAPHAQAMQRPPGALRIGFMTVSPLGTPVHPEAVAAVDDAARLLASLGHHVEE
                     AAPDIDGRLLASNYLHIYFGQVPAAMARAYAAGARRSEFEPMTRIIETLGKATSAGTL
                     TTNLAQWNQHARALARFHERCDLLLTPTLAHPPVRHGAGDPPAWQQAALDLLDRTGLL
                     GLFARSGLAAPTVDKIATDSLQYVPFTQLANLAGVPAMSVPLHWTADGLPLGVQFVAP
                     FGREDRLLQLAAQLEQARPWFGRLPGWVAEGREAPAASSAPGRTT"
BASE COUNT          297 a          782 c          762 g          259 t
ORIGIN      
        1 cacccagtgg catcgcgtca cgttctatga ccgcctggcc gagatcgccg gcgaatacct
       61 gaagaagggc aagcccgtct acatcgaggg ccgcctgaag tacggcaagt acaccgacaa
      121 ggacggcatc gagcgcaaca ccgtcgacat cgtcgccgag cagatgcaga tgctgggcgg
      181 ccgcgatggc ggcggtggcg gcgacgaagg cggcggcggc agcggcggcc gctacctcgc
      241 cccgcgcgac ggcgtcgacg gcaacgagga ccgcaacgcg tcgcgcccgg ccgcgcgtcc
      301 cgcccccgcg ccgcgtccgg cgcccaaggc gtcgaccggg ttcgatgaca tggacgacga
      361 catcccgttc tgagaccgcc cggttccact ctcctgaagg gcctcggcgc aagccgcagg
      421 ccctttttcc tgcgccggcg gaacggccgc ggcccgggtg gccacgcgca tcccgtcacg
      481 gcgtccgggc gcggcgtgca cccgggtgtc catgggcgcg ctcatgttgt ccgtcccggg
      541 gcggacgagg ccgctggcgc ctcgcgccct tccgccaccc accccggcaa ccgaccgaac
      601 cacggccgcg cctgctccag ctgcgcggcc agctgcagca gccggtcctc gcggccgaac
      661 ggcgccacga actgcacgcc cagcggaagg ccgtcggccg tccagtgcag cggcacgctc
      721 atcgccggca cgccggcgag attggccagc tgcgtgaacg gcacgtactg caggctgtcg
      781 gtggcgatct tgtccacggt cggcgccgcc agccccgagc gcgcgaacag gcccagcagg
      841 ccggtgcgat cgagcaggtc gagcgcggcc tgctgccacg ccggcggatc gcccgcgccg
      901 tggcgcacgg gcgggtgcgc cagcgtgggc gtcagcagca ggtcgcagcg ttcgtggaac
      961 cgggccagcg cgcgggcatg ctggttccac tgagccaggt tcgtcgtgag cgtgcccgcg
     1021 gacgtggcct tgccgagcgt ctcgatgatg cgcgtcatcg gctcgaactc gctgcgtctt
     1081 gcgcccgccg cgtacgcgcg cgccatcgcg gcgggcacct ggccgaagta gatgtgcagg
     1141 tagttgctcg cgagcagccg gccgtcgatg tcgggcgcgg cctcctccac gtggtggccg
     1201 agcgacgcga gcagccgcgc ggcgtcgtcg acggcggcca ccgcttccgg gtgcacgggc
     1261 gtgcccagcg gcgacaccgt catgaagccg atgcgcagcg cgcccggcgg gcgctgcatc
     1321 gcctgtgcat ggggcgcgtc cggcggcgcg atcacgaccg ggtcgccggg ctcggcgccg
     1381 gcgagcgcgt ccagcatgcg ggcggtgtcg cgcacgctgc gcgagatcac gccctcgctc
     1441 gacgcgccga accacgcctc gccgatgccc gggccggacg acacgcgacc gcgcgacggc
     1501 cgcagcccga acagcccgca gcacgcggcc gggatgcgga tcgagccgcc gccgtccgcc
     1561 gcggcggcca tcggcaacac gcccgacgcg atcgcggccg cggcgccgcc gctcgatccg
     1621 ccgggcacgt gctcgacgtt ccacggattg ttggtgcgac cattggcgcg cgagtcggtg
     1681 acgcccttga gcgcgaactc gggcaggttg gtcttgccga agatcaccag ccccgcggcg
     1741 aggtagcggc gcacgacgta ggccgtctcg gtgggcacga cgtgccgcat gccggcgctg
     1801 ccgtagccgg tgggcaggcc cgcgacgtcc tgcacgccgt ccttgagcag gaagggcacg
     1861 cccgtgaacg ggcccccggg caacggcgcg gcgagctggg cgcgcgcctc ggcgtccatc
     1921 agccggcaga cggcattgat gcgcggctgc gtgcggtgca gccgggccag cgcgacagca
     1981 tcgcatcgca aataatccgt gagattcacg cggcctcccc atccaccgtc ttgttgagct
     2041 catcgtagct cgtttccggc ggcaaacaat accggccggc gacgaaagcg gccccaatgc
//
LOCUS       DATCRO010000139        10821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_294078, whole genome shotgun sequence.
ACCESSION   DATCRO010000139 DATCRO010000000
VERSION     DATCRO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294078"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..871
                     /gene="fabG"
                     /locus_tag="VIN75_03835"
     CDS             113..871
                     /gene="fabG"
                     /locus_tag="VIN75_03835"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8973322.1"
                     /translation="MTETRDRDRDGRGTVLVTGASRGIGRAIALAAARDGHDVVVHCR
                     SGLEQANAVCAEIAALGATARVLAFDVADRAGAAAALQADIAGHGAYYGVVCNAGIAR
                     DNAFPAMSGEEWDAVVHTNLDGFYNVLHPVVMPMVQRRKPGRIVTLASVSALMGNRGQ
                     TNYSAAKAGVIAATKSLAIELAKRNITVNCVAPGLIDTEMVPPEVLEEAMKIIPLRRM
                     GTPDEVAALVAFLLRPEAAYITRQVISVNGGMLG"
     gene            868..2094
                     /locus_tag="VIN75_03840"
     CDS             868..2094
                     /locus_tag="VIN75_03840"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY8973323.1"
                     /translation="MKRVVVTGFGALSPLGHDWPTVLANLKGGRNAVQVMEAWAQYDG
                     LNTRLGAPALPFELPAHYNRKSTRSMGRVALMATRASELALADAGLLGDPLVTSGRMG
                     VSYGSSAGTPAAIGDFGRMLHDKSTEGINANTYIKMMSHTAAVNIGVFFGLTGRIVTT
                     SSACTSGSQGIGYAFEAIQSGRQVAMLAGGSEELDATEAAVFDTLFATSVRNDAPALT
                     PRPFDARRDGLVLGEGACTLVLEELEHALARGARIHAELLGWGTNSDGTHVTHPNAAT
                     MAQAMRMALVDARLTPGSIGYVNAHGTATEQGDMAESQATSEVLGPHVPISSLKSYMG
                     HTLGACGALEAWMTIEMMREGWFAPTLNLEEVDPRCGALDYITGDGRVIETDVVMSNN
                     FAFGGINTSLIFRRWS"
     gene            2251..2610
                     /locus_tag="VIN75_03845"
     CDS             2251..2610
                     /locus_tag="VIN75_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973324.1"
                     /translation="MARSAEMLELGRQTIVSADSQPLTLQAVRKAIIAGGAVHTWKPV
                     ADQPGVLTLEADSGQHQVVVDVAYDAQGYQITYKSSANMNYEHTDKRTTIHPKYNKWI
                     QDLNSAIRSATLTAKSE"
     gene            2648..3085
                     /locus_tag="VIN75_03850"
     CDS             2648..3085
                     /locus_tag="VIN75_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ATPase subunit"
                     /protein_id="HEY8973325.1"
                     /translation="MKKLVVVPLLVLIAAAPAFARNNAVTVTLDSVLDMPEAKAKLDG
                     SVKFYLAGAPTPKIIKKFGEDTSNAKTNAFNKTPEEACRWNALSALLKLQEHAKRDGA
                     NAVVDIVSYRDHVDFKSATEIECSDGAFLGGIALKGTYATVGK"
     gene            3098..3799
                     /locus_tag="VIN75_03855"
     CDS             3098..3799
                     /locus_tag="VIN75_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8973326.1"
                     /translation="MLRLAVASVTPAADDIPAPMPAWAGGAERRRWQALRGRPRAAFI
                     ASRLLVRELLASATGVPAAAWEVSAEAGVAPVARSPEAANTVHVSLSHRLGWVAAAVA
                     DAPVGVDIECDRPARSEPDERAALMLAPGELTEWRRLAEGERQAALLARWTAKEAWFK
                     ASPPQAAPWDFRRVQAREGGPADAHANVRTWRAAPLHVAVCCADPRALAAARCEGLPR
                     VDDERWWQVAAAPSL"
     gene            3823..4542
                     /locus_tag="VIN75_03860"
     CDS             3823..4542
                     /locus_tag="VIN75_03860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8973327.1"
                     /translation="MSIAKPLPDYIPETAFGDWFLRTRTWDVHVLEVALRDLARLDPS
                     PRANHPVVVDVGCGWGGSLRKLAERFKPERLVGFDIAPKMVAAATQEAAQVRLANGRT
                     AEVIQGDSARLPLPDASVDLLFCHQTFHHLVDQDRAIAEFFRVLRPGGKLLFAESTRR
                     YIHSWIIRLLFRHPMQMQKTAPQYLALVRAAGFAVPDAQVSYPFLWWSREDLGLVERV
                     LRIKPPAVREETLINLVATKP"
     gene            4773..6977
                     /locus_tag="VIN75_03865"
     CDS             4773..6977
                     /locus_tag="VIN75_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY8973328.1"
                     /translation="MSKNPAPSRAARGIAGRDALDVHNTVAWHGAGLSDHQALVDKWA
                     ALGFRTLSLSIYDEPAHPLLAAVMVRRAAIHAEHQVCPRTLAQLQDDVRANEKIGRGL
                     YLVAATGAAGHAAYAASFRPMNPLPVVRLELGKADFRSFNAAQQQAGRILQWVDVFGD
                     DADPRFAAIWASNPGKVAWSAEAFDPAAGQQAPTVDATRLQQRFDAITSTRGRPLHIA
                     LASSDRTAECFVDSTIGPWVARGAMTPAQYQAEFDKQAKAGLMPLQVSARGSGANARI
                     AALFASREETDPRTLRDQGPVTVASIDAVMHQYLQAENLRGAALAIARGGRLVYAKGY
                     TYAEAGYPDLAPQTPFRQASCSKTFAAVAMMRLIQDIPSVTLDTKVQAVLHLKQPDGS
                     APLDPRWADITIGHLLRSDSGIDQGLMYGSAAAAQAFGAPLPATHAQLLSYATTKLLT
                     GRPGDPRNSAYGNFDYMMLGEVIAKLHGSGSFVQALQALVLAPLHQARTHASRSRVTD
                     QPAGDARHHMTVYDPAGGWPLYPFEVLPTVREPGGRLVPTHYGALDYEMFSSAGGLSA
                     SVVDMARLGAMFSVDADNPVLAPATIRQQLQGAVDATGELATASGDPSHGYFGFDWAS
                     RDATGAFTASKGGWLPGQGTVLQVTTGGFTYALAINGNADVKVDWMTPIAAAAQGHAW
                     ADDDLFQTAYGMPSLAPAGRHAARPDAAAPRPASALASVRASMAVGRNGLRC"
     gene            7068..7739
                     /locus_tag="VIN75_03870"
     CDS             7068..7739
                     /locus_tag="VIN75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY8973329.1"
                     /translation="MSTSTFEDRPQTLGEEIANAISHGLGFLLAIASLPILVVMAVRH
                     GTATNIVAVSIFSVTMMLLYLTSTLYHAIPDGKVKAVLNRIDHAAIYLFIAGSYTPFA
                     LGVLNGAWGWTLFGAIWAMAAAGIAVKLMNLLKHPLWSTGLYVAMGWTAIIAAGPLVH
                     AMPMAGLLWLLAGGVAYTLGAVVFMFDSRFKYAHFAWHLFVLLGTTCHFFAALWYATP
                     ARVAA"
     gene            7868..8353
                     /locus_tag="VIN75_03875"
     CDS             7868..8353
                     /locus_tag="VIN75_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973330.1"
                     /translation="MSGFIEILHDERVAADPETVHAHYVDLHHRGLARVHPRERLRER
                     APGPAGPRFECVARIGWSKGLDVWERHERPDGSVLDQCVAGPHWGRSIIARFPPDPQA
                     ADAGTVLELTLTQPLRPMLARVLGRLLRARLEAELRQFAAEIRADVERGWRPQRHLRA
                     A"
     gene            complement(8363..8755)
                     /locus_tag="VIN75_03880"
     CDS             complement(8363..8755)
                     /locus_tag="VIN75_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8973331.1"
                     /translation="MSDSPNPAGEPLLVACLCAQWCRTCDAYRDTLLAARESLQLHPE
                     VAARFLWVDIEDESDLIGDLDIEDFPTLLLARGDAILFFGPILPHAQTIDRLLRGAVA
                     GELPPLSPDALAPDVRALPGRLRGHAAL"
     gene            9114..9839
                     /locus_tag="VIN75_03885"
     CDS             9114..9839
                     /locus_tag="VIN75_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8973332.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKEWTRVIREIMVAARTGGGDPG
                     MNPRLRLAIEKAKAVNLPADTVKRNIDKATGNLEGVNYEEIRYEGRGIGGAAVIIDTM
                     TDNKVRTVAEVRHAFSKYGGVMGTEGSVAFQFRHVGQFIFAPGASEDRIMEVALEAGA
                     DDVITNDDGSIEVLSAPAGFETVKNALEAAGLKPDGAEVTMRPESNVELTGEDSARMQ
                     KLLDIIEELDDVQDVYHNAVLSE"
     gene            9836..>10821
                     /gene="purD"
                     /locus_tag="VIN75_03890"
     CDS             9836..>10821
                     /gene="purD"
                     /locus_tag="VIN75_03890"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8973333.1"
                     /translation="MKILVIGGGGREHALAWKLAQSERVQRVYVAPGNGGTAADKRLV
                     NVNITEPEALAQFALAEKIALTVVGPEGPLSQGVVDVFRSHGLRIFGPTKAAAQLESS
                     KAFSKNFMKRHGIPTAAYETFTDIAAAHAYVDKLGAPIVVKADGLAAGKGVVVATTVA
                     EAHAAVDWMLKPEGGLAVQHNDAPRVVIEEFLAGEEASFIVMVDGRNVVALASSQDHK
                     RLADGDTGPNTGGMGAYSPAPVVTPNVHAKVMHEIILPTVEGMAKDGIVFSGFLYAGL
                     MIDANGHPKTLEFNTRMGDPETQPIMMRLKSDLAEVLMHATDGTLDQVELQW"
BASE COUNT         1710 a         3866 c         3809 g         1436 t
ORIGIN      
        1 gccgcatcga gcacgccggc cgcgtgctgg cgagcggccg catcagcgtg ttcgagccgc
       61 ccgccgattc agagcaagcg tcgccatccg gcgcgccgag ggaatgaaga gaatgaccga
      121 gaccagggac agggacaggg acgggcgcgg cacggtgctg gtgaccggcg cgagccgcgg
      181 catcgggcgc gcgatcgcgc tcgcggccgc gcgcgacggc cacgacgtgg tggtgcattg
      241 ccgcagcggc ctcgagcagg cgaacgccgt ctgcgcggag atcgccgcgc tcggcgcgac
      301 ggcgcgcgtg ctggcgttcg acgtcgccga ccgcgccggc gccgccgccg cgctgcaggc
      361 cgacatcgcc gggcatggcg cctactacgg cgtcgtctgc aacgccggca tcgcgcgcga
      421 caacgcgttc ccggcgatga gcggcgagga gtgggacgcg gtcgtccaca ccaacctcga
      481 cggcttctac aacgtgctgc atccggtggt gatgcccatg gtccaacgcc gcaagccggg
      541 ccgcatcgtg acgctcgcat cggtgtcggc gctgatgggc aaccgcgggc agaccaacta
      601 cagtgccgcc aaggcgggcg tgatcgcggc gacgaagtcg ctcgccatcg agctggccaa
      661 gcgcaacatc accgtcaatt gcgtcgcgcc ggggctgatc gacaccgaga tggtgccgcc
      721 cgaggtgctg gaggaagcga tgaagatcat cccgttgcgt cgcatgggca cgcccgacga
      781 ggtggccgcg ctggtcgcgt tcctgctccg gcccgaggcg gcgtacatca cgcgccaggt
      841 catctccgtc aacgggggca tgctcggatg aagcgcgtcg tcgtcaccgg attcggggcg
      901 ctgagccccc tgggccacga ctggcccacc gtgctcgcca acctcaaggg cggccgcaac
      961 gccgtccagg tcatggaggc ctgggcgcag tacgacggcc tgaacacgcg cctgggcgcg
     1021 ccggcgctgc cgttcgagct gcccgcgcat tacaaccgca agtccacgcg cagcatgggc
     1081 cgggtggcgc tgatggccac gcgcgcgagc gagctggcgc tcgccgacgc cgggctgctg
     1141 ggcgacccgc tggtcaccag cgggcgcatg ggcgtgtcct atggctcgtc ggccggcacg
     1201 ccggcggcca tcggcgactt cggccgcatg ctgcacgaca agtccaccga gggcatcaac
     1261 gcgaacacgt acatcaagat gatgtcgcac accgcggcgg tcaacatcgg cgtgttcttc
     1321 ggtctgacgg gccgcatcgt caccacgtcg agcgcctgca cctcgggcag ccagggcatc
     1381 ggctatgcgt tcgaggcgat ccagagcggg cgccaggtcg cgatgctggc cggcggctcc
     1441 gaggaactcg acgccaccga ggcggccgtg ttcgacacgc tgttcgccac cagcgtgcgc
     1501 aacgacgcgc cggcgctcac cccgcgcccg ttcgacgcca ggcgcgacgg cctggtgctg
     1561 ggcgagggcg cctgcacgct ggtgctggag gagctcgagc acgcgctggc ccgcggtgcg
     1621 cgcatccacg ccgaactgct gggctggggc accaattccg acggcaccca cgtcacgcac
     1681 ccgaacgcgg cgacgatggc gcaggccatg cggatggcgc tggtcgacgc gcgcctgacg
     1741 cccggctcga tcggctacgt caacgcgcac ggcaccgcca ccgagcaggg cgacatggcc
     1801 gaatcgcagg ccaccagcga ggtgctgggc ccgcacgtgc cgatcagctc gctcaagagc
     1861 tacatgggcc acaccctcgg cgcctgcggc gcgctggagg cgtggatgac catcgagatg
     1921 atgcgcgagg gctggttcgc cccgacgctg aacctcgagg aggtcgaccc gcgctgcggc
     1981 gcgctcgact acatcaccgg cgacggccgc gtcatcgaga ccgacgtcgt catgagcaac
     2041 aatttcgcgt tcggcgggat caacacctcg ttgatcttcc ggcgctggtc atgaatgcgg
     2101 gcgcattgcc cgcttgcccc gagaatgact gaccgagccc gcggccgcgg gacgaaccac
     2161 gacctggaga ggagccagac gatgatgatg agaacccccc ttcgcgcgct gttgttggcc
     2221 gctgccctga cgaccgcagg cggtgccgcg atggcgcgct ccgcggagat gctcgaactg
     2281 ggtcgccaga cgatcgtcag cgccgactcc cagcccctga cattgcaggc cgtgcgcaag
     2341 gccatcatcg cgggcggcgc ggttcacacc tggaagccgg tcgccgacca gcccggcgta
     2401 ctcacgctgg aggccgacag cggtcagcac caggtggttg tcgacgtggc ctacgacgct
     2461 cagggctacc agatcaccta caagagcagc gccaacatga actacgagca cacggacaag
     2521 aggacgacga tccacccgaa gtacaacaag tggatccagg acctcaacag cgcgatccgc
     2581 agtgccacgc tcaccgccaa gagcgagtaa cacgaaccgc ccgccccacc cccaaggaga
     2641 ttcatccatg aagaagctcg tcgtcgttcc cctgctcgtg ctcatcgcgg ccgcgcccgc
     2701 gttcgcgcgc aacaatgccg tcacggtgac gctcgattcg gtgctggaca tgccggaggc
     2761 caaggccaag ctggacggct ccgtcaagtt ctacctcgcc ggcgcgccca ctccgaagat
     2821 catcaagaag ttcggtgaag acacgtccaa cgccaagacg aacgcgttca acaagacgcc
     2881 cgaggaggcc tgtcgctgga acgcgctgag cgcgctgctc aagctgcagg aacatgccaa
     2941 gcgcgacggc gccaatgcgg tggtggacat cgtcagttac cgcgaccacg tggacttcaa
     3001 gagcgccacc gagatcgagt gctccgacgg tgccttcctg ggcggcatcg cgctgaaggg
     3061 cacctatgcc accgtcggca agtaagctgg ccccgtgatg ttgcggctcg ccgtggcctc
     3121 ggtgacgccg gcggcggacg atatccctgc gccgatgccc gcctgggcgg ggggcgccga
     3181 aaggcgacgc tggcaggcgc tgcgcgggcg gccgcgcgcg gccttcatcg ccagccgcct
     3241 actggtgcgc gaactgctgg cgtcggccac tggcgtgcca gccgccgcat gggaggtgtc
     3301 cgccgaggcg ggggtcgcgc cggtggcgcg ttcgcccgag gcagcgaaca cggtccatgt
     3361 cagcctgtcg catcggcttg gctgggtcgc cgcggcggtg gcggatgcac ctgtaggtgt
     3421 cgacatcgaa tgcgaccgcc ctgcccgcag cgaaccggac gagcgtgccg cattgatgct
     3481 ggcacccggc gaactgaccg agtggcgcag gctggccgag ggcgaacggc aagccgcgct
     3541 gctggcgcga tggacggcca aggaagcgtg gttcaaggcc agcccgccgc aggccgcgcc
     3601 ctgggatttc cgtcgcgtgc aggcgcgcga gggcggcccc gcggacgcgc acgcgaacgt
     3661 gcgcacgtgg cgcgcggcgc ccctgcacgt ggccgtctgc tgcgccgacc cgcgcgcgct
     3721 ggccgcggcg cgctgcgagg gcctgccgcg cgtcgacgac gaacgctggt ggcaggtcgc
     3781 tgccgcgccg tcgctttgaa gagaacgaat cgagcaagtc cgatgtccat cgccaagccc
     3841 ctgcccgact acatccccga gaccgcgttc ggggactggt tcctccggac gcgcacctgg
     3901 gacgtgcacg tgctcgaggt cgcgctgcgc gatctcgccc gcctggaccc gtcgccgcgc
     3961 gccaaccatc cggtggtggt cgacgtcggc tgcggctggg gcggctcgct gcgcaagctc
     4021 gccgagcgct tcaagcccga gcgcctcgtc ggcttcgaca tcgcgcccaa gatggtggcg
     4081 gccgcgacgc aggaggccgc gcaggtgcgg ctggcaaacg gccgcacggc cgaggtgatc
     4141 cagggcgaca gcgcgcggct gccgctgccc gacgcgagcg tcgacctgct gttctgccac
     4201 cagaccttcc accatctcgt cgaccaggat cgcgcgatcg cggagttctt ccgcgtgctc
     4261 aggccgggcg gcaagctgct gttcgcggag tccacgcggc gctacatcca ctcctggatc
     4321 atccgcctgc tgttccgtca tccgatgcag atgcagaaga ccgcgccgca atacctcgcg
     4381 ctggtgcgcg ccgcgggctt cgcggtgccg gacgcccagg tgtcgtaccc gttcctgtgg
     4441 tggagccgcg aggatctggg cctggtggag cgcgtgctgc gcatcaagcc gccggcggtg
     4501 cgcgaggaga cgctgatcaa cctcgtggcg acgaagccct gatctcacga aggggtcttg
     4561 ccttgccgcg atcgcgagag cgtcgcggct ggcggcggcg aggcagcgcg atcgtggaga
     4621 cgccggacgg ctgcggcgag ccatcgccga aatcgacggg cgaatcgcga tagtccctag
     4681 atcgcggtgc gcggttgtcc gccttccggg ggatggaggc gagggcggcg cgggccacag
     4741 tcccgtcacg ccatcaacac gagacgcccg ccatgtcgaa gaaccccgcc ccgtcccggg
     4801 ccgcccgcgg catcgcggga cgcgacgcgc tcgacgtcca caacaccgtc gcctggcacg
     4861 gcgccgggct gtccgaccac caggcgctgg tcgacaagtg ggcggccctc ggcttccgca
     4921 cgctgtcgct gtcgatctac gacgagcccg cgcatccgct gctggcggcc gtgatggtcc
     4981 ggcgcgcggc gatccacgcc gagcaccagg tgtgcccgcg cacgctggcg cagttgcagg
     5041 acgacgtgcg ggccaacgag aagatcggcc gcggccttta cctggttgcg gcgacggggg
     5101 ccgcgggcca cgccgcctac gccgccagct tccgcccgat gaacccgctg ccggtggtgc
     5161 ggctggagct gggcaaggcc gacttccggt cgttcaacgc ggcgcagcag caggccggcc
     5221 gcatcctgca atgggtggac gtgttcggcg acgacgccga tccgcgcttc gccgcgatct
     5281 gggcgtccaa tccgggcaag gtggcgtggt cggcggaggc gttcgatccc gccgccgggc
     5341 agcaggcgcc gacggtcgac gccacgcggc tgcagcaacg cttcgacgcc atcacgtcga
     5401 cccgcggccg tccgctgcac atcgcgctgg cctcgtcgga ccgcacggcc gaatgcttcg
     5461 tcgattccac gatcggcccg tgggtggcgc gcggcgcgat gacgccggcg cagtaccagg
     5521 ccgagttcga caagcaggcc aaggcgggcc tgatgccgct gcaggtgtcg gcgcgcggct
     5581 ccggcgcgaa cgcgcgcatc gccgcgctgt tcgcctcgcg cgaggagacc gacccgcgca
     5641 cgctgcgcga ccagggcccg gtcacggtcg cctcgatcga tgccgtcatg caccaatacc
     5701 tgcaggccga gaacctgcgc ggcgcggcgc tcgccatcgc gcgcggcggc cgcctcgtgt
     5761 acgccaaggg ctacacgtac gccgaggccg gctaccccga cttggcgccg cagacgccgt
     5821 tccgccaggc gagctgctcc aagaccttcg cggccgtcgc gatgatgcgg ctgatccagg
     5881 acatcccgtc cgtgaccctc gacacgaagg tgcaggccgt gctgcacctg aagcagccgg
     5941 acggcagcgc gccgctcgat ccgcgctggg ccgacatcac gatcggccac ctgctgcgca
     6001 gcgattcggg catcgaccag ggcctgatgt acggcagcgc cgctgccgcc caggccttcg
     6061 gcgcaccgct gccggccacg cacgcgcagc tgctcagcta cgccacgacg aagctgctga
     6121 ccggccggcc cggcgacccg cgcaactcgg cctatggcaa cttcgactac atgatgctgg
     6181 gcgaggtcat cgccaagctg cacggctcgg gcagcttcgt ccaggcgctg caggcgctcg
     6241 tgctcgcgcc gctgcaccag gcgcgcacgc acgcctcgcg cagccgcgtc accgatcagc
     6301 ccgcgggcga cgcgcgccac cacatgacgg tgtacgaccc cgccggcggc tggccgctgt
     6361 acccgttcga ggtgctgccc accgtgcgcg agcccggcgg caggctggtg cccacgcact
     6421 acggcgcgct cgactacgag atgttctcca gcgccggcgg cctgtcggcc tcggtggtgg
     6481 acatggcgcg cctgggcgcg atgttcagcg tcgatgcgga caacccggtg ctcgcgccgg
     6541 cgacgatccg ccagcaactc cagggcgcgg tcgacgccac cggcgagctc gcgaccgcgt
     6601 cgggcgatcc gtcgcacggc tacttcggct tcgactgggc gagcagggac gccaccggcg
     6661 cgttcaccgc gtccaagggc ggctggctgc cggggcaggg cacggtgctg caggtgacga
     6721 ccggcggctt cacctacgcg ctcgccatca acggcaacgc cgacgtgaag gtcgactgga
     6781 tgacgccgat cgccgccgcg gcccaaggcc acgcgtgggc cgacgacgac ctgttccaga
     6841 ccgcgtacgg catgccgtcg ctcgcgcccg cgggccggca cgcggccagg ccggacgccg
     6901 ccgcgccgcg gcccgcgtcc gcgctcgcga gcgtgcgcgc cagcatggcg gtcggccgca
     6961 acggcctgcg atgctgaggc cgcgacgggc ggcgcgagcg gcaaagccgg gcacggcctt
     7021 tgccttggcg ttacaaagcg cgtgaccaaa gccagtacga tcgcaccatg agcacctcca
     7081 cgttcgaaga ccgtccgcag acgcttggcg aagagatcgc caacgccatc agccacggcc
     7141 tgggcttcct gctggccatc gcgtcgctgc ccatcctcgt cgtcatggcc gtgcgccatg
     7201 gcaccgccac caacatcgtg gccgtcagca tcttctcggt gacgatgatg ctgctgtacc
     7261 tcacgtccac gctgtaccac gcgatcccgg acggcaaggt caaggccgtg ctcaatcgca
     7321 tcgaccacgc ggcgatctac ctgttcatcg cgggcagcta cacgccgttc gcgctcggcg
     7381 tgctgaacgg cgcctggggc tggacgctgt tcggcgccat ctgggcgatg gccgcggccg
     7441 gcatcgcggt caagctgatg aacctgctca agcacccgct gtggtccacc gggctgtacg
     7501 tggccatggg ctggacggcg atcatcgccg cgggcccgct ggtccacgcg atgcccatgg
     7561 ccggactgct gtggctgctg gccggcggcg tcgcctacac gctgggtgcg gtcgtgttca
     7621 tgttcgactc gcgcttcaag tacgcgcact tcgcctggca cctgttcgtg ctgctgggca
     7681 ccacctgcca cttcttcgcg gcgctgtggt acgccacgcc ggcgcgagtg gccgcctgag
     7741 ccgggcgtcc ctggcgcgcg tgcgggttcg gcgggatgcc gaatccggcg gcgtccttgc
     7801 cgcgtagccc ccacgaatgc gacaggccgg cggcgtccgc cggcacggca ttcgccccgc
     7861 ggaacgcatg tccggcttca tcgagatcct gcacgacgag cgggtggccg ccgatccgga
     7921 gacggtgcat gcgcactatg tcgacctgca ccaccgcggc ctcgcgcgcg tgcatccgcg
     7981 cgaacgcctg cgcgaacgcg cgcccggccc ggccggcccg cgcttcgaat gcgtggcgcg
     8041 catcggctgg agcaaggggc tggacgtctg ggagcgccac gaacggcccg acggcagcgt
     8101 cctggaccag tgcgtggccg gcccgcactg gggccgctcg atcatcgcgc gcttcccgcc
     8161 cgacccgcag gccgccgacg ccggcacggt gctcgagctg acgctcacgc agccgctgcg
     8221 gcccatgctc gcccgcgtcc tgggtcgcct gctgcgcgcg cgactcgagg ccgagctgcg
     8281 ccagttcgcg gcggagatcc gcgccgatgt cgagcgcggc tggcggccgc agcggcacct
     8341 gcgggcggcg tgacccggcg cttcagagtg ccgcgtgccc gcgcaatcgc cccggcaacg
     8401 cccgcacgtc cggcgcaagg gcgtccggcg acagcggcgg cagctcgccc gcgacggcgc
     8461 cgcgcagcag gcggtcgatg gtctgggcgt gcggcaggat ggggccgaag aacaggatcg
     8521 cgtcgccgcg cgcgagcagc agcgtgggga agtcctcgat gtcgaggtcg ccgatcaggt
     8581 ccgactcgtc ctcgatgtcg acccacagga agcgcgccgc gacctcgggg tgcagctgca
     8641 gcgactcgcg cgcagccagc agcgtgtcgc ggtaggcgtc gcaggtgcgg caccactggg
     8701 cgcacaggca ggcgaccagc agcggttcgc cggccgggtt cggggagtcg ctcatcgccg
     8761 ggagtttgcc agcagaaaag gaaaagggag cgggacaccc gtcccgcccc cttcacccgc
     8821 cagggcccgt caaccctggc gtcccgtcgc gccccttgcg ttggcgcgag gccagtgtgc
     8881 gacccgggcg ggctggcggg caacgaagca aacggcatga gctcggaagg cggcggcagc
     8941 atggcgactg ccggcgcgcc gcgcggaacg tgaccagcgc cgcgaacggg ccgggagcgc
     9001 gcgaccgtgc ggccggcgac cggcgtgcgg gcagctaaaa tgccggtttc ccgaaaaatc
     9061 cgaaccgccg ggcgcgacgc ccgcggcagc gcagactcca ggagcgtttt cttatggccg
     9121 ggcactcgaa atgggccaac atccagcatc gcaagggccg acaggacgag aagcgcggca
     9181 aggaatggac gcgggtcatc cgtgaaatca tggtagccgc gcgcaccggc ggcggcgacc
     9241 cgggcatgaa cccgcgcctg cgcctggcca tcgagaaggc caaggccgtc aacctgccgg
     9301 ccgacacggt caagcgcaac atcgacaagg ccacgggcaa cctcgaaggc gtgaactacg
     9361 aggagatccg ctacgagggc cggggcatcg gcggcgccgc cgtcatcatc gacacgatga
     9421 ccgacaacaa ggtgcgcacg gtggccgagg tgcgccatgc gttctccaag tacggcggcg
     9481 tcatgggcac cgaaggctcg gtggccttcc agttcaggca cgtcggccag ttcatcttcg
     9541 cgcccggcgc atcggaggac aggatcatgg aagtggcgct ggaagccggc gccgacgacg
     9601 tgatcaccaa cgacgacgga tcgatcgagg tgctcagcgc gccggccggg ttcgagaccg
     9661 tcaagaacgc gctggaggcc gccggcctca agcccgacgg cgccgaggtg accatgcgtc
     9721 ccgagagcaa cgtcgaactg accggcgagg acagcgcacg catgcagaag ctgctcgaca
     9781 tcatcgagga gctcgacgac gtccaggacg tctatcacaa cgcggtactt tccgaatgaa
     9841 gatcctcgtc atcggcggcg gcgggcgcga gcacgcgctg gcctggaagc tggcccagag
     9901 cgaacgggtg cagcgcgtct acgtggcccc gggcaacggc ggcaccgcgg cggacaagcg
     9961 cctggtcaac gtcaacatca ccgagcccga ggcgctggcg cagttcgcgc tggccgagaa
    10021 gatcgcgctg accgtggtgg gccccgaggg tccgctgtcg cagggcgtcg tcgacgtgtt
    10081 ccgctcgcac ggcctgcgca tcttcggccc gacgaaggcc gcggcgcagc tggagagctc
    10141 caaggcgttc tccaagaact tcatgaagcg ccacggcatc cccaccgcgg cctacgagac
    10201 cttcaccgac atcgctgccg cgcacgccta cgtcgacaag ctgggcgcgc ccatcgtcgt
    10261 caaggccgac ggcctggccg cgggcaaggg cgtggtcgtg gcgacgacgg tcgccgaggc
    10321 gcacgccgcg gtcgactgga tgctcaagcc cgagggcggc ctggcggtgc agcacaacga
    10381 cgcgccgcgc gtcgtcatcg aggaattcct cgccggcgag gaagccagct tcatcgtgat
    10441 ggtcgacggc aggaacgtcg tcgcgctcgc gtccagccag gaccacaagc gcctggccga
    10501 cggcgacacc ggccccaaca ccggcggcat gggcgcgtac tcgcccgcgc cggtcgtcac
    10561 gcccaacgtg cacgccaagg tgatgcacga gatcatcctg ccgaccgtcg aaggcatggc
    10621 caaggacggc atcgtgttca gcggcttcct gtacgcgggc ctgatgatcg atgccaacgg
    10681 ccacccgaag acgctcgagt tcaacacccg catgggcgat cccgagacgc agccgatcat
    10741 gatgcgcctc aagagcgacc tcgccgaggt gctgatgcac gccaccgacg gcacgctcga
    10801 ccaggtcgag ctgcaatggg a
//
LOCUS       DATCRO010000140         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_676612, whole genome shotgun sequence.
ACCESSION   DATCRO010000140 DATCRO010000000
VERSION     DATCRO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676612"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VIN75_03895"
     CDS             <1..144
                     /locus_tag="VIN75_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY8973334.1"
                     /translation="AQDFAQQHLADQRLPAAHKRGYTAVMALRSWTFGPLRELLRDPN
                     IEP"
     gene            complement(155..943)
                     /locus_tag="VIN75_03900"
     CDS             complement(155..943)
                     /locus_tag="VIN75_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8973335.1"
                     /translation="MARIYCIANQKGGVGKTTTTVNLAAGLALVGQRVLLIDLDPQGN
                     ATMGSGINKRDLPMTVYDVLLESATITEAAQKSGSGGYDVLGANRELAGAEVELVALE
                     RRNERLRRALKAVDADYDFVLIDCPPSLNLLTLNGLCSAHGVIVPMQCEYFALEGVSD
                     LVNTVKQVHANLNRDLELIGLLRVMYDPRITLQANVSEQLKAHFGDKVFNTVIPRNVR
                     LAEAPSYGQPGVVFDPTSRGAVAFVEFAQEMVAREKGPALAEAR"
     gene            complement(992..1702)
                     /gene="rsmG"
                     /locus_tag="VIN75_03905"
     CDS             complement(992..1702)
                     /gene="rsmG"
                     /locus_tag="VIN75_03905"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY8973336.1"
                     /translation="MPLEHAEPSRLDGRLEALAPRLALTLSARDRERLLAYMAMIQRW
                     TKVYNLTAVRDTDEMFTHHLLDCLAVVAPLRQGSGEAGQGALRVLDVGSGAGLPGVIL
                     ATLNPSWQVTCVDAVAKKAAFIRQAAAELALPNLHGVHGRVEAAGTFKTPEFDLVTSR
                     AFASIHDFTTLTRQLLAPRGQWAAMKAKLSADERAAVPADVEMFHVEQLEVPDLHENR
                     CLVWLRPRLPADQTARQP"
     gene            complement(1692..>2713)
                     /locus_tag="VIN75_03910"
     CDS             complement(1692..>2713)
                     /locus_tag="VIN75_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973337.1"
                     /translation="SLPFDVQLAAVRSMRGLENAHILRPGYAIEYDYFDPRGLKASFE
                     TRAIGGLFFAGQINGTTGYEEAAAQGLYAGVNAALLASGRADAFVLRRDQAYLGVLVD
                     DLITKGVTEPYRMFTSRAEFRLQLREDNADLRLTELGRQLGLVDDARWAHFNRKRDAL
                     ARETERLRSTWVHPNNLPAVDAERLLGKALEHEHSLSDLLRRPGVTYDTVVEATAVAR
                     PNVDVSRETLNRDLGEQLAGEVIEQLEIATKYAGYIDKQNEEVSRAQSLEELALPDEL
                     DYNQVTALSFEVRQKLNQHRPQTLGQATRISGVTPAAISLLLVHLKKKRFKGFTNPAP
                     AEHAA"
BASE COUNT          414 a          881 c          942 g          476 t
ORIGIN      
        1 gcccaggact ttgcccagca gcaccttgcc gaccagcgcc tgccggcggc acacaagcgg
       61 ggctacacgg cggtgatggc gctgcgctcc tggaccttcg gtccgctgcg cgagctgctg
      121 cgcgacccga acatcgagcc ctgaccaggc cggatcagcg agcctcggcg agggccgggc
      181 ccttttcgcg ggcgaccatt tcctgcgcga attccacgaa ggcgacggcg ccgcgtgagg
      241 tggggtcgaa cacgacgccc ggttgcccgt agctgggtgc ctcggcgagc cggacgttgc
      301 gcgggatcac ggtgttgaac accttgtcgc cgaagtgtgc cttgagctgc tcgctgacgt
      361 tggcctgcag cgtgatgcgc gggtcgtaca tgacgcgcag cagcccgatc agttccaggt
      421 cgcgattcag gttggcgtgc acctgcttga ccgtgttgac caggtcggac acgccctcca
      481 gcgcgaagta ctcgcactgc atcggcacga tgacgccgtg ggcgctgcac agcccgttca
      541 gcgtcagcag gttcagcgac ggcgggcagt cgatcaggac gaagtcgtag tcggcgtcga
      601 ccgccttgag ggcgcggcgc agacgctcgt tgcgccgctc cagcgccacc agttccacct
      661 cggcgccggc cagttcacga ttcgcgccca gcacgtcgta accaccgctg cccgacttct
      721 gggcggcttc ggtgatggtc gcgctctcca gcaggacgtc gtagaccgtc atcggcagat
      781 cgcgcttgtt gatgccggaa cccatggtcg cgttgccttg cgggtcgagg tcgatcagca
      841 ggacgcgttg tccaacgagc gccaagccgg ccgccaggtt gaccgtcgtc gtggtcttgc
      901 cgacgccgcc cttctggttc gcaatgcaat agattctcgc catggatgac cggtgaagcg
      961 ctgttggggg tggaagggct cgcgcctatt gtcagggctg gcgagcggtc tggtcagcgg
     1021 gaagacgtgg gcgtagccag acaaggcacc ggttttcgtg cagatccggc acctcgagtt
     1081 gttccacgtg aaacatctcg acgtcggccg gcacggcggc gcgttcgtcg gccgacagct
     1141 tggccttcat ggccgcccat tggccccgcg gagccagcag ctgccgcgtc aacgtcgtga
     1201 agtcgtggat ggaggcgaag gcgcgcgagg tgacgaggtc gaactcgggc gtcttgaagg
     1261 tgcccgcggc ctcgacccgg ccgtgcacgc cgtgaagatt tggcagcgcc agttcagccg
     1321 ccgcttggcg aatgaaggcc gccttcttgg ccaccgcgtc gacgcaggtc acctgccagg
     1381 acgggttgag cgttgccagg atgacgcccg gcagtcccgc tccgctgccc acgtcgagga
     1441 ctcggagcgc gccctgcccg gcttcgccgc tgccctggcg cagaggggcg accaccgcga
     1501 ggcagtcgag cagatgatgg gtgaacatct cgtccgtgtc gcgcacggcc gtcaggttgt
     1561 agaccttggt ccagcgctgg atcatggcca tgtaggccag caggcgttcg cgatcccggg
     1621 cggacaaggt cagggccagt cgaggcgcca gcgcttcgag tcgtccgtcc aggcgggacg
     1681 gctcggcgtg ttcaagcggc atgttcggca ggagcggggt tcgtgaagcc cttgaagcgc
     1741 ttcttcttca aatggacaag tagcagcgag atggcggcgg gggtgacgcc ggagatgcgg
     1801 gtcgcctgtc cgagcgtttg tggccggtgc tgattcagct tctggcgaac ttcgaacgac
     1861 agcgcggtga cctggttgta gtccagctcg tcgggcaggg cgagctcttc caggctctgc
     1921 gcccggctga cttcctcgtt ctgcttgtcg atgtagccgg cgtacttcgt ggcgatctcg
     1981 agttgttcga tgacctcgcc cgccaactgc tcgccaaggt cacggttcag tgtttcacgt
     2041 gaaacgtcga cgttcgggcg cgcaacggcg gtggcctcga cgaccgtgtc gtacgtgacc
     2101 ccgggccgcc gcaggaggtc ggacagcgaa tgctcgtgtt ccagcgcctt gcccagcaga
     2161 cgctccgcgt ccacggccgg aaggttgttc gggtgtaccc aggtcgaacg caggcgctcc
     2221 gtctcgcggg ccagcgcgtc gcgcttgcga ttgaaatgcg cccaacgggc gtcgtccacc
     2281 aaaccgagct ggcgacccag ctccgtcagg cgcaggtcgg cgttgtcctc gcgcagctgg
     2341 agccgaaact cggcccggct ggtaaacatg cggtagggct ccgtgacccc cttggtgatc
     2401 aggtcgtcga cgagcacgcc caggtaggcc tggtctcgcc gcaggacgaa ggcgtccgcc
     2461 cggccactgg ccaggagggc cgcattcacg ccggcgtaga ggccttgcgc cgccgcctcc
     2521 tcgtagccgg tggtgccgtt gatctggccg gcaaagaaca gtccgccgat cgcgcgcgtc
     2581 tcgaagctcg ccttcaggcc acgcggatcg aagtagtcgt actcgatcgc atagccgggg
     2641 cgcaggatgt gcgcgttctc cagcccgcgc atcgagcgca cggctgccag ctgcacgtcg
     2701 aacggcaggc tgg
//
LOCUS       DATCRO010000141         6667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_406441, whole genome shotgun sequence.
ACCESSION   DATCRO010000141 DATCRO010000000
VERSION     DATCRO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406441"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VIN75_03915"
     CDS             <1..865
                     /locus_tag="VIN75_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8973338.1"
                     /translation="IESAQRSTQLQQEALDVSVRLQQAGRVGVSDAARARSQLALLQA
                     ALPPLQARRQAALFRLATLTGELPQDFDRDVAACTAPPRVAGTIPVGDGAALLRRRPD
                     VRQAERRLAASTARIGVATADLYPRVTLGLSAGSEGLGNDFLGKDTFSYGVGPLISWT
                     VPDTGVVRAHIAQAGADADAALARLDGTVLAALRETQTALEVYARELDRHAALQAARD
                     ESATVAEQARRLYRSGRTGYLDALDAERGLAASEATLAASDAQLADDQVSLFLALGGG
                     WEPAPERTAAR"
     gene            924..1313
                     /locus_tag="VIN75_03920"
     CDS             924..1313
                     /locus_tag="VIN75_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973339.1"
                     /translation="MPPVPQEASSPRDRENPSAHDPALARDHDAGPDRERLSPSERAL
                     APEGDAPMQSHNAVADDGTYVGQAGGYMGGASPAVESGRAQVNEANRRGDGAYGFPAG
                     PGVGMPGGEHQPAAQKPDDLPNQYPQR"
     gene            complement(1508..1750)
                     /locus_tag="VIN75_03925"
     CDS             complement(1508..1750)
                     /locus_tag="VIN75_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973340.1"
                     /translation="MITIISSMNVNPRDGTPRLAGHAGARAGRRRFGRPPSGADRGLV
                     IMGTHYSGIGPGRRVRAAPREPPALKTVGFHDGGPL"
     gene            1737..2147
                     /locus_tag="VIN75_03930"
     CDS             1737..2147
                     /locus_tag="VIN75_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8973341.1"
                     /translation="MIVIILIGILVAIALPYYGDYRNRVRTAQATSDIIGNEAVIEAY
                     FAINKTLPNTWADIPQARTIDPWGRPYVYYNIQANGKGGARKDHALNPLNTDYDMYSP
                     GADGLTKAQITQKDSVDDVIRASNGGYVGLASGF"
     gene            2152..4890
                     /locus_tag="VIN75_03935"
     CDS             2152..4890
                     /locus_tag="VIN75_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973342.1"
                     /translation="MPLRRARALLDRHLEWRLLAVFLAAALIPLAASDWIATSVLDGI
                     VQRIGHDRDNLAARTVARQVFDRLLLSRTLLLAVDEAPGTARARLAASAAGPQGAFSH
                     VACGDSQDAQWAGLQAQWAAARSGASAPAQAGLGAGLRLAPAPSGNAGLLMATGPYGA
                     PRCIAILNPDHVWETVHDQADDSAWHVRADDGSDVLAWHGVDARPGDAAGAARFSTHL
                     FLGSEFGAGNWTLEQRTPPRTVGWHGMPVGVWLACVAAATLLLIGLAARSTIRRTLAP
                     LAALTDGARRLAAGIEPARVEVRRDDELGKLATSFNDMASQLEQRIASLRALGQIDAG
                     ILQGTPFTDLACGVLERLASVRPDARVAVAWPVSPTLLATVRYPAPVATGAERDEICE
                     LRPDAMARFTDLDDGLLPVSTLHGLEAVVACDPADLHAAPCLVLGVRDGQANRALITI
                     HGIDLEAMRDEAQSLRDRLAVAVVARDRQDRLEHRATHDPLTGLNNTDGLQRALAPLL
                     EGDDDVAVLFIDLDHFKDVNDCYGHATGDRLLQAAARRLRRGTADGALISRSGGDEFV
                     VVLPATDAAQARRTAERILESLNQPFIIAATEHRTGASIGIAMYPAHALDREDLLRCA
                     DIAMYESKRLGRNRATVFEPALDTQVRERNELLSALAHAVERSEFVLHYQPRLDAATG
                     RVAAAEALVRWQHPERGLLLPGVFIDLAETSGHIDALGRNVMASAIARIAQWHNDGRS
                     IERISVNVSQHQFADGQLVSHVQALLRQHGVPGTRLEIEVTESVLGGDIASVRRQLHQ
                     LRDLGVTVAMDDFGTGYSSLAQLRTLPIDVMKVDRAFVKDLETDPAAVAIVRTIVTLA
                     RELGLQVVAEGIETPAQAALLAAIGCDQFQGFLYSRPVPPDECAALGPFEMPPAGR"
     gene            5011..>6667
                     /locus_tag="VIN75_03940"
     CDS             5011..>6667
                     /locus_tag="VIN75_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8973343.1"
                     /translation="MRRLAWAVGAVLVLWLVTWLAVPPLVRWQAPRQLSAALGRDVTL
                     GGVDFHPWALEVVLHDLAVAAAPAASGTASSPAPAASAAPLLQVARVRANVALSSIFR
                     RAPVIEAIDIDQARIALARTAPGHYDIDDLIRRFAPKPDARPSEPVRFALYNLQVHDL
                     ALRLDDRPAGHVHVVDKFELSLPFLSNLPANVDITVQPHLAFRLNGAPFDSAAQATPF
                     AATDHGMLKLTLADLDVRPWLGYLPESLPLRLNAGALSADLSLAFSQPARGATSMTLK
                     GWVAARDVAASSVGGQPLLAWKQVRVGLADVQPLAHKLAFDSVKLDGVQWFLDRDASG
                     AVDPLREGTTPRPAAPPAKTAAAASAPASAAEASPSAPWDVRVAALDIAGSRIVWNDA
                     STKPAATLVLDDLALQAKAIAWPSTAPATFTASAKVATGATNAAAAASATGAARLALE
                     GKAGSSDAQVAITLTDLSLQAFAPYLAQALVPRVDGRLGAKASLTWSDKAGASALKIA
                     LAEASLDALRVQPPGAPQAASWDRLDVSDLQVDVSGRTLTLGGV"
BASE COUNT          908 a         2588 c         2348 g          823 t
ORIGIN      
        1 catcgagagc gcgcagcgct cgacgcaact gcagcaggag gcgctggacg tgtcggttcg
       61 cctgcagcag gccggccgcg tgggcgtcag cgacgcggcg cgcgcccgca gccagctcgc
      121 gctgctgcag gccgcgctgc cgccgttgca ggcgcgccgc caggccgcgc tgttcaggct
      181 cgccacgctc accggcgagc tgccgcagga cttcgaccgc gacgtcgcgg cctgcaccgc
      241 gcccccgcgc gtggccggca cgatccccgt gggcgacggt gccgcgctgc tcaggcgccg
      301 tcccgacgtg cgccaggccg agcgccgcct cgccgcatcg accgcccgca tcggcgtggc
      361 cacggccgac ctctacccac gcgtgacgct gggcctgtcg gccggctccg agggcctggg
      421 caacgacttc ctcggcaagg acaccttcag ctacggcgtg ggcccactga tttcctggac
      481 ggtgcccgat accggcgtcg tgcgggcgca catcgcgcag gcgggcgccg acgccgacgc
      541 ggccctcgcg cgcttggacg gcacggtgct ggccgccctg cgcgagacgc agacggcgct
      601 cgaggtctat gcccgcgagc tggaccgcca tgccgcgttg caggccgcgc gcgacgagag
      661 cgccaccgtc gctgagcagg cgcgccggct gtatcgcagc ggccgcacag gctatctcga
      721 cgcgctcgac gccgagcgcg gcctggcggc cagcgaggcc acgctggccg cctccgatgc
      781 gcagctggcc gacgaccagg tcagcctgtt cctggcgctc ggaggcggct gggagccggc
      841 gccggagcgc acggccgcgc gctgaggcac ccggtttgcc ctggaagggc gcgccgcatc
      901 cgggcgcgcc acaggacatc gccatgccgc ccgtgcccca ggaagccagc tcgccccgag
      961 atcgcgagaa ccccagcgcc cacgaccccg ccctggcgcg cgaccacgac gccggccccg
     1021 accgcgagcg gctgagcccg tccgaacgtg ccctcgcgcc cgagggcgac gccccgatgc
     1081 agagccacaa cgcggtcgcc gacgacggca cctacgtggg ccaggccggc ggctacatgg
     1141 gcggcgcgtc gcccgcggtg gagagcggcc gggcgcaggt caacgaggcc aaccggcgcg
     1201 gcgacggcgc ctacggcttc ccggcgggcc ccggcgtggg catgcccggc ggcgagcacc
     1261 agccggcggc gcagaagccc gacgacctgc ccaaccagta tccgcaacgc tgaacgacag
     1321 aaaagcagcc cggaggtgag tccgggctgc gaagccggca gccagggagg aaagaggaag
     1381 gttgcgctgc cgggataccc gtcaaccagg tacccgtgca gtggaacctg gtcgcgtatc
     1441 ccgggtttgg ccactttgca tcaggacgga catacggccg agttgcgggg cgaacctgaa
     1501 cgcgcgatca gagtggcccc ccatcgtgaa atcccaccgt tttcagcgcc ggcggctcgc
     1561 gcggagccgc ccggacgcga cggcccggcc cgatgccaga ataatgcgtg cccatgatga
     1621 caagaccgcg atcggcgccg gagggcggcc gaccgaaccg acgcctgccg gcgcgcgccc
     1681 cggcgtgccc cgccagccgc ggcgtgccgt cgcgcgggtt cacgttcatc gagctgatga
     1741 tcgtgatcat cctgatcggc atcctcgtgg cgatcgccct tccctactac ggcgactatc
     1801 gcaaccgcgt caggacggcc caggccacca gcgacatcat cggcaacgaa gccgtcatcg
     1861 aagcctactt cgcgatcaac aagacgctgc cgaacacgtg ggccgacatc ccgcaggccc
     1921 gcaccatcga cccctggggg cgcccgtacg tctactacaa catccaggcc aacggcaagg
     1981 gcggcgcgcg caaggaccac gcgctcaacc cgctcaacac cgactacgac atgtacagcc
     2041 cgggtgcgga cggcctgacc aaggcgcaga tcacgcagaa ggacagcgtc gacgatgtga
     2101 tccgcgccag caacggcggc tacgtcggcc ttgcctcggg cttctgaggc catgccgctg
     2161 cgtcgcgcgc gcgccctgct ggatcgccac ctcgagtggc gcctgctggc cgtcttcctg
     2221 gccgccgcgc tgatcccgct ggccgcgtcc gactggatcg cgacctccgt cctcgacggc
     2281 atcgtccagc gcatcgggca cgaccgcgac aacctcgccg cgcgcaccgt cgcgcgccag
     2341 gtgttcgacc gcctgctgct ctcgcgcacc ttgctgctgg cggtcgacga ggcgcccggg
     2401 accgcgcgcg cccgcctggc cgccagcgcc gccgggccgc agggcgcgtt ctcgcacgtg
     2461 gcctgcggcg actcgcagga cgcgcagtgg gccggcctgc aggcgcagtg ggccgccgcc
     2521 cgcagcggcg cctccgcgcc ggcgcaagcc ggcctgggag ccggcctgcg cctcgcgccc
     2581 gcgccgtccg gcaacgccgg attgctgatg gccacggggc cctacggcgc accacgctgc
     2641 atcgcgatcc tgaatcccga ccacgtctgg gagaccgtcc atgaccaggc cgacgacagt
     2701 gcgtggcacg tgcgcgccga cgacggcagc gacgtgctcg cctggcacgg cgtcgacgcg
     2761 cgccccggcg acgcggcagg cgccgcgcgc ttcagcacgc acctgttcct cggctcggag
     2821 ttcggcgccg gcaactggac gctcgagcaa cgcacgccgc cgcgcaccgt cggctggcac
     2881 ggcatgcccg tgggcgtgtg gctcgcctgc gtggccgcgg ccacgctgct gctgatcggc
     2941 ctggccgcgc gcagcaccat ccgccgcacg ctggcgccgc tggccgcgct gaccgacggc
     3001 gcgcgccggc tcgcggccgg catcgagccc gcccgcgtgg aggtccgccg cgacgacgag
     3061 ctcggcaagc tggccacctc gttcaacgac atggcctcgc agctggagca gcgcatcgcg
     3121 tcgctgcgcg cactcggcca gatcgacgcc ggcatcctgc agggcacgcc gttcaccgac
     3181 ctcgcctgcg gggtgctgga gcgcctcgcc agcgtccggc ccgatgcgcg cgtcgcggtc
     3241 gcctggccgg tctcgcccac cctgctcgcg accgtgcgct acccggcgcc cgtggccacc
     3301 ggcgccgagc gcgacgagat ctgcgagttg cgtcccgacg cgatggcgcg cttcaccgac
     3361 ctggacgatg gcctgctgcc ggtgtcgacg ctgcatggcc tggaggccgt cgtcgcctgc
     3421 gacccggccg acctgcacgc cgcgccctgc ctggtgctgg gcgtgcgcga cggtcaggcc
     3481 aatcgcgcgc tgatcacgat ccacggcatc gacctcgagg ccatgcgcga cgaggcgcag
     3541 tccctgcgcg accggctggc cgtcgcggtg gtcgcgcgcg accgccagga ccggctcgag
     3601 caccgcgcca cgcacgaccc gctgaccggc ctgaacaaca ccgacggcct gcagcgcgcg
     3661 ctcgcgccgc tgctcgaggg cgacgacgac gtcgccgtgc tgttcatcga cctcgaccac
     3721 ttcaaggatg tcaacgactg ctacggccac gccaccggcg accgcctgct gcaggccgcc
     3781 gcgcgccgcc tgcgccgcgg cacggccgac ggcgcgctga tctcgcgcag cggcggcgac
     3841 gagttcgtcg tcgtgctgcc cgccaccgac gcggcgcagg cgcgcaggac ggccgagcgc
     3901 atcctcgagt cgctgaacca gcccttcatc atcgcggcca ccgagcaccg caccggcgcg
     3961 agcatcggga tcgcgatgta cccggcgcac gcgctcgatc gcgaggacct gctgcgctgc
     4021 gccgacatcg cgatgtacga gtccaagcgc ctgggccgca accgcgccac cgtgttcgag
     4081 cccgcgctcg acacgcaggt gcgcgagcgc aacgagctgc tgtcggcgtt ggcgcacgcg
     4141 gtcgagcggt cggagttcgt cctgcactac cagccgcgcc tggacgccgc caccgggcgc
     4201 gtggccgccg ccgaggcgct ggtgcgctgg cagcaccccg agcgcggcct gctgctgccg
     4261 ggcgtgttca tcgacctcgc ggaaacctcc ggccacatcg atgcgctggg ccgcaacgtg
     4321 atggccagcg ccatcgcccg catcgcgcag tggcacaacg acggccgctc gatcgagcgg
     4381 atctcggtca acgtctcgca gcaccagttc gccgacggcc agctggtgtc gcacgtccag
     4441 gcgctgctgc gccagcacgg cgtgccgggc acccggctcg agatcgaggt gaccgagagc
     4501 gtgctgggcg gcgacatcgc cagcgtgcgg cgccagctgc accagctgcg cgacctgggc
     4561 gtcaccgtcg cgatggacga cttcggcacc ggctattcgt cgctggcgca gctgcgcacg
     4621 ctgccgatcg acgtcatgaa ggtcgaccgc gcgttcgtca aggacctgga gaccgacccc
     4681 gcggccgtgg ccatcgtgcg caccatcgtg acgctggcgc gcgagctggg cctgcaggtg
     4741 gtggccgagg gcatcgagac gccggctcag gccgcgctgc tggccgccat cggctgcgac
     4801 cagttccagg gcttcctgta ctcgcggccc gtgccgcccg acgagtgcgc cgcgctcgga
     4861 ccgttcgaga tgcccccggc cgggcgctga acctgcgccg gccgtgcggc accgccacac
     4921 acggatacaa cggacatcga aatcccacca tactccccac ttgcgccgcg acgaagacgg
     4981 cgccgggagc gagatgtgaa gggcaaatgg atgcgccgac tggcctgggc agtgggcgcc
     5041 gtgctggtgc tgtggctggt gacgtggctg gcggtgccgc cgctggtcag gtggcaggcg
     5101 cccaggcagc tgtcggctgc gctcgggcgc gacgtcacgc tggggggcgt cgacttccat
     5161 ccgtgggcgc tcgaggtggt gctgcatgac ctcgccgtcg ccgccgcgcc cgcggcgtcc
     5221 ggcaccgcat cgtcccccgc cccggccgcc tccgccgcgc cgctgctgca ggtggcccgc
     5281 gtgcgcgcca acgtggcgct gtcgtcgatc ttccggcgcg cgccggtcat cgaggcgatc
     5341 gacatcgacc aggcccgcat cgcgctggcc cgcaccgcgc cgggccacta cgacatcgac
     5401 gacctgatcc ggcgcttcgc gcccaagccc gacgccaggc cgtccgaacc cgtgcgcttt
     5461 gccctctaca acctgcaggt gcacgacctc gccctgcgct tggacgaccg gcccgcgggg
     5521 cacgtgcacg tcgtcgacaa gttcgagctg tcgcttcctt tcctgtcgaa cctgccggcc
     5581 aacgtcgaca tcaccgtgca gccgcacctg gcgttccgcc tgaacggcgc gccgttcgac
     5641 agcgccgcgc aggccacgcc gttcgccgcc accgaccacg gcatgctcaa gctgacgctg
     5701 gccgacctcg acgtgcgccc ctggctcggc tacctgcccg agtcgctgcc gctgcggctg
     5761 aatgcgggcg cgctgtcggc cgacctgtcg ctcgccttct cgcagcccgc gcgtggcgcg
     5821 accagcatga cgctcaaggg ctgggtcgcc gcgcgcgacg tcgcggccag cagcgtgggc
     5881 ggccagccgc tgctcgcgtg gaagcaggtg cgcgtgggcc tggccgacgt gcagccgctc
     5941 gctcacaagc tggcgttcga cagcgtcaag ctggacggcg tgcagtggtt cctcgatcgc
     6001 gacgcgagcg gcgccgtcga cccgctgcgc gagggcacga cgcccaggcc ggccgcgccg
     6061 cccgcgaaga cggccgccgc ggccagcgcc ccggccagcg cggccgaggc gtcgccatcg
     6121 gcgccgtggg acgtgcgcgt cgcggccctg gacatcgccg gcagccgcat cgtctggaac
     6181 gacgcctcga cgaagccggc cgccacgctg gtgctggacg acctggcgct gcaggccaag
     6241 gccatcgcgt ggccgtcgac ggcgcccgcc acgttcacgg catcggccaa ggtcgcgacc
     6301 ggcgcgacca atgcggccgc cgccgcctcg gcgaccggcg ccgcgcggct ggcgctcgag
     6361 ggcaaggccg gcagcagcga cgcgcaggtc gcgatcacgc tgaccgacct gtcgctgcag
     6421 gcgttcgcgc cctacctcgc acaggcgctc gtgccgcgcg tcgacgggcg cctgggcgcc
     6481 aaggcgtcgc tgacgtggtc ggacaaggcc ggcgcatccg cgctgaagat cgcgctggcc
     6541 gaggcctcgc tggacgcgct gcgcgtgcag ccgcccggcg cgccgcaggc cgcgagctgg
     6601 gatcgcctgg acgtcagcga cctgcaggtc gacgtgtccg gccgcacgct gacgctgggc
     6661 ggcgtca
//
LOCUS       DATCRO010000142         7561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_541692, whole genome shotgun sequence.
ACCESSION   DATCRO010000142 DATCRO010000000
VERSION     DATCRO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541692"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VIN75_03945"
     CDS             complement(<1..1172)
                     /locus_tag="VIN75_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8973344.1"
                     /translation="MLAARKVLVCPARACMELLPDGRFRLEPCGGAIDRQIDRTLRSV
                     SRSFGSRAIAVVLTGMGNDGAAGASELHAAGGRVLVQTESSAENPEMPRAARDAGAAD
                     LVVPLADIAQVLTDLATGKVWPKARTELEAIERVFGTEGDIAALARDIDWLHTPLGPV
                     LLWPPELRQSVRTVVDSPHPGAVWWGREFVQVYNDAWRRFTGPSKHPQAFGGVARETW
                     REIWREIEPMAERVMGEGVASSVEDFPIVIDRNGYLEEVVANLAFSPIRDEAGALVGV
                     YCTCWETTGKVVADRRLAALRALVAQAAGASTPREACERAAAALACGPLDLPFMLIYL
                     FDSGRRQATLAGVAGLAPGSTAAPHTLPLFGAHDTWALQRLLETVADTAGAGVLLE"
     gene            complement(1560..2408)
                     /locus_tag="VIN75_03950"
     CDS             complement(1560..2408)
                     /locus_tag="VIN75_03950"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8973345.1"
                     /translation="MADTSLVKRALAPAWVRPEALNVTPALLGAPLAPHARRAAAMGV
                     DVALIGLLSSTGMFWVVAGIGALAVQLRTRTPGRPRSRTVFMWVALAVLLLVAYDHGT
                     DWWAHRNDPPHATSAKDDDDDDVASAAAAASSNAATVALVAAEKQHVQDAARIRDLEA
                     QLAAARQPRPLQWRDEAMRRLHRLGLRFGWAIAYFSLLPFWWKGQTVGKRLFGLRVVH
                     LAGKPMGVMDCFGRYGGYAAGLATGGIGLLQALWDPNRQAVEDKLAHTVVIDLRGPRA
                     LAPPQA"
     gene            complement(2414..3085)
                     /locus_tag="VIN75_03955"
     CDS             complement(2414..3085)
                     /locus_tag="VIN75_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8973346.1"
                     /translation="MTRPALPDRAFAAFLFDMDGTLLSSIACAERVWARWARRHGVDV
                     ASFLPTIHGKRAIDTVGRLGLPGVDAAAEAAIIHAEEMVDVEGIEQIAGVAAFVGALP
                     VDRWAVVTSAPRELARRRIAAAGLPMPPLMIAAEDVINGKPAPDCFELAASRLGVSAR
                     DCLVFEDAPAGIAAAEAAGASVLVITATHAHPLDTRHPVVGDYAGFAPQVDADGALRL
                     VPLAG"
     gene            complement(3120..4001)
                     /locus_tag="VIN75_03960"
     CDS             complement(3120..4001)
                     /locus_tag="VIN75_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8973347.1"
                     /translation="MRLPFLACLATLLAALVACSTSLAPAAPAPRHSTAGPGVHVIAR
                     RLAMPGLGRERTIRLYLPPSYDTSPGKRYPVIYMHDAQNLFDEATSSYGQEWGVDETL
                     DAFARTRGFEAIVVGIDHGGDERIHELSPWTNPKYGPAQGEQYMAFVVDVVKPYVDAR
                     YRTLADRAHTAMAGSSLGGLETHYALLRYPQVFGQAAIFSPSYWYADEVYAQTKARPW
                     PADTRVYLYIGGHEGDESVPDVQRMMALLAASGRATTDIALHIEPAAEHDERAWRAEF
                     PRAVAWLFGVRPLDATP"
     gene            complement(4037..5341)
                     /locus_tag="VIN75_03965"
     CDS             complement(4037..5341)
                     /locus_tag="VIN75_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8973348.1"
                     /translation="MTTTHDAPADPLQAAIAHHRRGELDAAQAIYAELLRVDPRRADA
                     LNFMGVLMVQRGERERALELLRKASMIAPREPGVWNNLGNALLSLGKLPEAEKAFRRS
                     LALAESAEALTNLARIQRRRQEWARSEASARRAIALAPRSGEAWHYLSLALLGLQRPE
                     EAFEAAVQAELLLPAHAHRREAYGRALWAAGQHDHAVAFYRDWLDKEPGNPYAQHHLA
                     AALGQTPERASDAYVEKVFDQFAASFDTQLAALKYRAPELVAEAMAAALPAPAAQFDI
                     ADLGCGTGLCGPLLKPWARSLAGCDLSAGMLERAAPRAVYDELVKAELVRFLVERPAA
                     FDVLVSADTLIYFGELAAMLAAAHAALRPGGTLVFTLEALEDADDADFRLTESGRYAH
                     ALPGLRARLGAAGFAAPAISSITPRMESARAVAGWLVTVRRD"
     gene            5464..6747
                     /locus_tag="VIN75_03970"
     CDS             5464..6747
                     /locus_tag="VIN75_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LanC-like protein"
                     /protein_id="HEY8973349.1"
                     /translation="MLHDPARHEPLQAIAWDEARVRATIARIVDGTVAAFTPDGLWPP
                     HPRDLEPDDDPDMRATNLYWGAAGVIWALDYLRRVGAADSPPFARHLQAVRARNRAAL
                     GDDNAGSYLMGDLPFAMMAWGQAPDAARADAIAALIEGNIGHPARELMWGSPGSLLAA
                     AMMHGATDDERWAVLFRRIAQRLREQLLWSERQACHYWSQDLYGRTSRYIDAVHGFVA
                     TARALLMGRHLLPEAEWLEWQACIETTVANTAIRANGRATWPVQADEAPGTPQKWLMQ
                     FCHGAPGFVICLADLPGHALEPLLAEAGEATWAAGPLAKGSNLCHGTAGNAYAFLVLL
                     ARTRDARWRERARAFAMHAIGQFEAETARAGRLRHSLWTGDIGLAIFLWDCLHETPRF
                     PTLDVFFANEGRLRGLASPHVAAGLPPSRGKQPAT"
     gene            complement(6897..7394)
                     /locus_tag="VIN75_03975"
     CDS             complement(6897..7394)
                     /locus_tag="VIN75_03975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973350.1"
                     /translation="MTPVHPVPFVWRPVTPADVFTLAAIYRDCALRMGPQVYTPEQSR
                     AWASSAEDEAGFRDYVLTADTWIAERVGDVRAIGFCGASRDGELREIHSLYVTPRMTR
                     RGIGAAMLRRTLQRTEHEGATRFAAWATPFSRPVFLAAGFVLAQTVQAPYRGVMFERY
                     RVEKG"
BASE COUNT         1046 a         2791 c         2773 g          951 t
ORIGIN      
        1 tcctcgagca gcacgcctgc gcccgccgtg tccgcgacgg tttcgagcag gcgctgcaag
       61 gcccatgtgt cgtgcgcgcc gaacagtggc agcgtgtggg gcgcggccgt gctgccgggt
      121 gccagcccgg cgacaccggc cagcgtcgcc tgccggcgtc cgctgtcgaa gagatagatc
      181 agcatgaacg gcaggtccag cgggccgcag gccagcgccg ccgccgccct ttcgcaggcc
      241 tcgcgcggcg tgctcgcgcc ggcggcctgc gccacgagcg cgcgcaacgc cgcgaggcgc
      301 cggtcggcca ccaccttgcc ggtcgtctcc cagcacgtgc agtacacgcc gaccagggcg
      361 cccgcctcgt cgcggatcgg cgagaaggcg aggttggcga cgacctcctc gagatagccg
      421 ttgcggtcga tgacgatcgg gaagtcctcg acgctggagg ccaccccctc gcccatcacc
      481 cgctcggcca tcggctcgat ctcgcgccag atctcgcgcc aggtctcgcg cgcgacgccg
      541 ccgaacgcct gcggatgctt gctcggaccg gtgaaccgcc gccacgcgtc gttgtagacc
      601 tgcacgaact cgcgccccca ccagacggcg ccggggtggg gcgaatcgac gacggtgcgc
      661 acgctctggc gcagctcggg cggccacagc agcacggggc cgagcggcgt gtgcagccag
      721 tcgatgtcgc gcgcgagcgc ggcgatgtcg ccctcggtgc cgaacacgcg ctcgatggcc
      781 tccagctccg tgcgtgcctt gggccagacc ttgccggtgg ccaggtcggt cagcacctgt
      841 gcgatgtcgg caagggggac gacgaggtcc gcggcgccgg cgtcacgcgc cgcacgcggc
      901 atctccgggt tttctgccga ggactcggtc tgcacgagca cgcgccctcc tgcggcgtgc
      961 agctcggacg cgccggccgc gccgtcgttg cccatgcccg tcaggacgac cgcgatcgcc
     1021 cgggagccga agctgcgcga cacggagcgc aacgtgcgat cgatctgcct gtcgatcgcc
     1081 ccgccgcagg gctcgaggcg gaaacggccg tcgggcagca gttccatgca ggcgcgcgcg
     1141 ggacagacca gcaccttgcg cgccgccagc atcgacagct cgtccccccc aattcgaacg
     1201 cgaacggggc gcgccgggcg tagaagtcca ccgccgacga atccgggtgc aggtgctgga
     1261 cgacgacgat cggcacgtcg aagtccagcg gcaggccccg caggatcgtg aggatcgcga
     1321 cctggccgcc cgcggacgca gcgagcacga cgacgccgaa gccggtcgct tcgggcagcg
     1381 agatcgacct tgccttcatg caacgctcgg ccgcctggac ggacgtacca gagtcaaaag
     1441 cggatcgtac gctcgcggga gcgactctgg atgacccagt gccgtcccgg tgcgctcgac
     1501 cgttccgggg cggtgctttc ggcccgccgc cgccgtgccg gcggagtctc ccgggtgacc
     1561 tacgcctgcg gcggcgccag cgcgcgcggc ccgcgcaggt cgatgaccac cgtgtgggcc
     1621 agcttgtcct cgaccgcctg gcggttggga tcccacagtg cctgcagcag gccgatgccg
     1681 ccggtggcca ggccggccgc gtagccgccg tagcggccga agcagtccat cacgcccatg
     1741 ggcttgcccg ccaggtgcac gacgcgcagt ccgaacagcc gcttgcccac ggtctggccc
     1801 ttccaccaga agggcagcag cgagaagtag gcgatggccc agccgaagcg caggcccagc
     1861 ctgtgcagcc ggcgcatcgc ctcgtcgcgc cactgcagcg ggcggggctg gcgcgcggcg
     1921 gccagctgcg cctcgagatc ccggatgcgc gccgcgtcct ggacgtgctg cttctccgcc
     1981 gcgaccaagg cgacggttgc cgcgttcgag gacgccgccg cagccgcgga cgcgacgtcg
     2041 tcgtcatcgt cgtccttcgc ggaagtcgcg tgcggcggat cgttgcgatg cgcccaccag
     2101 tccgtcccgt ggtcgtacgc gaccagcagc agcaccgcca gcgccaccca catgaagacg
     2161 gtgcgcgagc gcgggcggcc cggcgtgcgc gtgcgcaact gcacggccag ggcgccgatg
     2221 ccggcgacga cccagaacat gccggtggac gacagcagtc cgatgagcgc gacatccacg
     2281 cccatcgccg ccgcgcgcct ggcgtgtggc gcgagcggtg cgcccagcag cgcgggggtc
     2341 acgttgagcg cttccgggcg cacccaggcg ggtgccagcg cgcgcttgac gagcgacgtg
     2401 tccgccatcg tcttcagccc gccagcggca cgagccgcaa cgcgccgtcg gcgtcgacct
     2461 gcggcgcgaa gccggcgtag tcgccgacga ccgggtggcg cgtgtcaagc ggatgcgcgt
     2521 gcgtcgcggt gatcaccagc acgctggcgc cggcggcctc ggcggcggcg atgcccgccg
     2581 gggcatcctc gaacacgagg cagtcgcgcg ccgacacgcc cagccgcgac gccgccagct
     2641 cgaagcagtc gggggccggc ttgccgttga tgacgtcctc ggccgcgatc atcagcggcg
     2701 gcatcggcag gcccgcggcg gcgatgcggc gccgcgcgag ctcgcgcggc gcggacgtga
     2761 cgaccgccca gcggtcgacc ggcagcgcgc ccacgaacgc ggccacgccg gcgatctgct
     2821 cgatgccttc gacgtcgacc atctcctcgg cgtggatgat ggcggcctcg gccgcggcat
     2881 cgacacccgg caggccgagc cggcccaccg tgtcgatggc gcgcttgccg tggatcgtcg
     2941 gcagaaacga cgcgacgtcc acgccatggc gccgggccca gcgggcccac acgcgctcgg
     3001 cgcaggcgat cgagctgagg agcgtgccgt ccatgtcgaa caggaaggcg gcgaaggcgc
     3061 ggtcaggcag ggcgggacgg gtcaagacgg gatcgctgga agacggcaag gccgacatct
     3121 tagggcgtcg cgtccagcgg ccgcacgccg aacagccagg ccaccgcgcg cgggaactcg
     3181 gcgcgccagg cgcgctcgtc gtgctccgcg gcgggctcga tgtgcagcgc gatgtcggtc
     3241 gtcgcccggc cggacgcggc cagcagcgcc atcatgcgct ggacgtcggg caccgactcg
     3301 tcgccctcgt ggccgccgat gtagaggtag acgcgcgtgt cggccggcca cgggcgggcc
     3361 ttggtctgcg cgtagacctc gtccgcgtac cagtacgacg gggagaagat cgcggcctgg
     3421 ccgaacacct gggggtatcg cagcaaggcg taatgcgtct ccaggccgcc cagcgagctg
     3481 ccggccatcg cggtgtgcgc gcggtcggcc agcgtccggt agcgcgcatc gacgtacggc
     3541 ttgacgacgt cgaccacgaa ggccatgtac tgctcgccct gcgcggggcc gtacttcggg
     3601 ttggtccagg gcgacagctc gtggatgcgc tcgtcgccgc cgtggtcgat gcccacgacg
     3661 atcgcctcga agccgcgcgt gcgggcgaag gcgtccagcg tctcgtcgac gccccactcc
     3721 tgcccgtagg acgacgtcgc ctcgtcgaac aggttctgtg cgtcgtgcat gtagatcacg
     3781 gggtagcgct tgcccggcga ggtgtcgtag gacggcggca ggtacaggcg gatcgtgcgc
     3841 tcgcgcccga ggccgggcat cgccaggcgt cgtgcgatga cgtgcacgcc ggggccggcg
     3901 gtcgagtggc gcggcgcggg ggccgccggg gccagggaag tcgagcacgc gacgagggcg
     3961 gcaagcagcg tcgccaggca ggcaaggaac ggaaggcgca tcccgccagg gtagcctgaa
     4021 atgcggacgc ctcgcctcag tcgcggcgca ccgtcaccag ccagcccgcc acggcgcggg
     4081 cgctctccat gcgcggcgtg atcgacgaga tcgccggcgc cgcgaagccc gccgcgccca
     4141 ggcgcgcgcg cagccccggc agcgcatggg cgtagcggcc gctttcggtc agccggaagt
     4201 cggcgtcgtc cgcgtcctcc agcgcctcga gcgtgaacac gagcgtgccg cccgggcgca
     4261 gcgccgcgtg cgcggcggcg agcatggcgg cgagctcgcc gaagtagatc agcgtgtcgg
     4321 ccgagaccag cacgtcgaac gccgccggcc gctccacgag gaaccgcacc agctcggcct
     4381 tcacgagctc gtcgtagacg gcacgcggcg ccgctcgctc gagcatgccg gccgacaggt
     4441 cgcagcccgc cagcgagcgg gcccacggct tgagcagcgg cccgcacagg ccggtgccgc
     4501 agccgaggtc ggcgatgtcg aactgcgcgg cgggcgccgg cagcgccgcg gccatcgcct
     4561 cggcgacgag ctcgggcgcg cggtacttca gcgcggcgag ctgcgtgtcg aagctcgcgg
     4621 cgaactggtc gaacaccttc tcgacgtagg cgtcgctcgc gcgctcgggc gtctgcccga
     4681 gcgccgccgc caggtggtgc tgcgcgtacg ggttgcccgg ctccttgtcg agccagtcgc
     4741 ggtagaacgc caccgcgtga tcgtgctggc ccgccgccca cagcgcccgg ccgtaggcct
     4801 cgcgccggtg cgcgtgcgcg ggaagcagca gctcggcctg caccgcggcc tcgaacgcct
     4861 cctcgggccg ttgcaggccc agcagcgcca gcgacaggta gtgccaggcc tcgcccgacc
     4921 gtggcgccag cgcgatcgcg cgccgggccg acgcctcgct gcgcgcccat tcctggcgcc
     4981 gccgctggat gcgcgcgagg ttggtcagcg cctcggcgct ctcggccagc gcgaggctgc
     5041 ggcggaacgc cttctcggcc tcgggcagct tgcccagcga cagcagcgcg ttgcccaggt
     5101 tgttccacac gccgggctcg cgcggggcga tcatcgacgc cttcctgagc agctccagcg
     5161 cgcgctcgcg ctcgccgcgc tgcaccatca gcacgcccat gaagttcagc gcgtcggcgc
     5221 gccgcgggtc gacccgcagc agctcggcgt agatggcctg cgcggcgtcg agctcgccgc
     5281 ggcggtgatg ggcgatggcg gcctggagcg ggtctgcggg ggcgtcgtgc gtggtcgtca
     5341 agcgggtctc cggggcggcg gcgagtgcgc caggatgcca tggatcggag gcgccgcggc
     5401 gcggccgata ccctctcggg gttcgaggcg taggatgccc gcacacgtct tcgcgaggcc
     5461 cccatgctgc acgaccccgc ccgccacgaa ccgctgcagg ccatcgcctg ggacgaggcc
     5521 cgcgtccgcg ccacgatcgc gcgcatcgtc gacgggacgg tcgccgcgtt caccccggac
     5581 ggcctgtggc cgccgcatcc gcgcgacctc gagccggacg acgacccgga catgcgcgcc
     5641 accaatctct actggggcgc agcgggcgtg atctgggcgc tggactacct gcgccgcgtc
     5701 ggggccgccg acagcccgcc gttcgcccgg cacctccagg cagtgcgcgc gcgcaaccgc
     5761 gccgcgctgg gcgacgacaa cgccggcagc tacctgatgg gcgacctgcc gttcgcgatg
     5821 atggcctggg gccaggcgcc ggacgcggcg cgcgccgacg cgatcgccgc gctcatcgaa
     5881 ggcaacatcg gccatcccgc gcgcgagctg atgtggggct cgcccggctc gctgctggcg
     5941 gccgcgatga tgcacggcgc gacggacgac gagcgctggg ccgtcctgtt ccgccgcatc
     6001 gcgcagcgcc tgcgcgaaca actgctgtgg tcggagcgcc aggcctgcca ctactggtcc
     6061 caggacctct acggccgcac gtcgcgctac atcgacgccg tgcacggctt cgtcgcgacg
     6121 gcccgagcgc tgctgatggg ccgccacctg ctgcccgagg ccgagtggct ggagtggcag
     6181 gcctgcatcg agacgacggt ggccaacacc gcgatccgcg cgaacggccg cgccacctgg
     6241 cccgtccagg ccgacgaggc gcccggcacg ccgcagaagt ggctgatgca gttctgccac
     6301 ggcgccccgg gcttcgtcat ctgcctggcc gacctgccgg gccacgcgct ggaaccgctg
     6361 ctggccgagg ccggcgaagc cacgtgggcc gccggcccgc tggccaaggg ctcgaacctg
     6421 tgccacggca ccgccggcaa cgcctacgcg ttcctggtgc tgctcgcgcg cacccgcgac
     6481 gcccgctggc gcgagcgcgc ccgcgcgttc gcgatgcacg cgatcgggca gttcgaggcc
     6541 gagaccgcac gcgccggccg cctgcggcat tcgctgtgga cgggcgatat cgggctggcg
     6601 atcttcctgt gggattgcct gcacgagacg ccgcggtttc cgaccctgga tgtgttcttt
     6661 gcgaacgagg gacgcctgag gggtctggcc tctcctcatg tcgccgccgg gctgccgcca
     6721 tcgcggggca agcagccggc gacatgaggc gaaggcctga cctcgatccc cggccgatga
     6781 tcggggtcag gccttcgcct cccgatcgcg atccgaaccg aggcttgcgg cggcatcgtg
     6841 gcgcgatcag gaggagaggc cggacccctc cgacctcggc gcggccgcaa gcagcctcaa
     6901 cccttctcga cccgataccg ctcgaacatc actccccggt acggcgcctg caccgtctgc
     6961 gccagcacga agccggcggc caggaagacg ggcctgctga acggggtggc ccaggcggcg
     7021 aagcgggtgg cgccctcgtg ctcggtgcgc tgcagcgtgc ggcgcaacat cgcggcgccg
     7081 atgccgcggc gggtcatccg gggcgtgacg tacagcgaat ggatctcgcg cagctcgccg
     7141 tcgcggctgg cgccgcagaa gccgatggcc cgcacgtcgc ccacgcgctc ggcgatccag
     7201 gtgtcggccg tcaggacata gtcgcggaat cccgcctcgt cctcggccga cgaggcccag
     7261 gcccgcgact gctcgggcgt gtagacctgc ggccccatcc gcagcgcgca gtcgcggtag
     7321 atcgcggcca gcgtgaagac gtccgccggc gtcacggggc gccagacgaa cgggaccgga
     7381 tggacaggcg tcatcacgcc gtcagctgaa caggcgccgc gcgcccggcg agccggcggc
     7441 caggtcgtgc gccatcagcg cctcgtacag gcgcgtcagt tcggcgtgga tcgcggagaa
     7501 cgcatgcagc gtgatcggct gcccctggtc gtcgacgatc gcggcatcga gatcgtcggc
     7561 c
//
LOCUS       DATCRO010000143         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_946488, whole genome shotgun sequence.
ACCESSION   DATCRO010000143 DATCRO010000000
VERSION     DATCRO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946488"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..746
                     /locus_tag="VIN75_03980"
     CDS             126..746
                     /locus_tag="VIN75_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973351.1"
                     /translation="MHPCREYWGQDVAYGSLVTGEPTPRAGRFGARTEIWILDTQDAS
                     RPAPHAQLAGLMRRIAGLPAGEEALRQSAAHIKDFLQAGKAAAPAPAGRPPGVRGECV
                     DEDGRILGIATLHRTPMLPPGSFAAAAGVASREEREEASAQLCAEFMRHLSALGEADG
                     VSRIDWVMVRATGTADGARSHLGATRVGPVALKPFAPGAGEAAAED"
     gene            complement(772..1260)
                     /locus_tag="VIN75_03985"
     CDS             complement(772..1260)
                     /locus_tag="VIN75_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY8973352.1"
                     /translation="MKVLDLRCATGHAFEGWFASEDDYGSQRERGMLDCPVCGNAEVV
                     RLPSAPRLNLSGARERAPTPARVAAGEAAGNEPAAPPVDMALQGGAVRRFVEAVAELL
                     SSTEDVGSRFAEEARRIHYGESEAKAIRGQASAEEREALADEGIEVFTLPSGLPPKSS
                     LQ"
     gene            complement(1271..1861)
                     /locus_tag="VIN75_03990"
     CDS             complement(1271..1861)
                     /locus_tag="VIN75_03990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8973353.1"
                     /translation="MRAQEGVDAHLVERLVRSENVYDGHFLKVRRDQVKLPDGQLAWR
                     EHIVHPGAVMIVPIADDGQLVVERQHRYPLDHVLLEFPAGKLDPNETTLACARRELLE
                     ETGYTAREWAYAGRLHNAPAYSNEFIEIWFARGLVAGAQQLDAGEFLEVFTMPEAALL
                     AQSASGELTDAKTMVALLKLQQWRSGQWEPTWQSVE"
     gene            complement(1919..>3331)
                     /gene="nuoN"
                     /locus_tag="VIN75_03995"
     CDS             complement(1919..>3331)
                     /gene="nuoN"
                     /locus_tag="VIN75_03995"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY8973354.1"
                     /translation="LVGVNASDKRLGLTYWIAQISLLAFSLMHLYELQAGRALYGMQR
                     MVVIDPLGHLLGLAAGLATMITLGFARPYLKSRDLLKGEFLSLAMFSLLGVSVMVSAN
                     NFLVVYIGLELMTLSLYALTALRRDHAVSTEAAMKYFILGALASGFLLYGLSMMYGAA
                     GTLDIPNVLEALHKGTVNPQVLVFGLVFIVAGLAFKLGLAPFHMWVPDVYQGAPTAVT
                     LLISGAPKLAAFGITFRILVEGLSGQAKDWEEMLIVLSVVSMTLGNLAAIAQSNLKRL
                     LAYSAIAQLGFMLLGFTPTVVDMKTVAAANAYSSALFYIITYVLTTLGTFGLIMVLAR
                     NGFESEEIADLAGLGKRSPWIAGVMSLFMFSLAGIPPLVGFYAKFAVLQALVSTGNQT
                     YIVLAVIAVLLSLVGAYYYLRVVKTMFFDEPVDPSPVRGETGATVLLALSGIATLGLG
                     LLPDGLMSVCRDAVLKALNS"
BASE COUNT          523 a         1183 c         1181 g          444 t
ORIGIN      
        1 gtgcatggcc gtccagcgac acggcattgg atcgccggcc tccggccctc tacagtcccg
       61 ccacgacaga cgcgccggcg ccccaagagg cccgcacggc gacgcacgga gacaagcgtt
      121 cacacatgca tccctgccga gaatactggg gccaggacgt cgcctacggg tcgctcgtca
      181 ccggcgagcc cacgccccgc gccggccgtt tcggcgcccg caccgagatc tggatcctcg
      241 acacgcagga cgcctcgcgt cccgccccgc acgcccagct cgcgggcctg atgcggcgca
      301 tcgccggcct gcccgccggc gaggaggcgc tgcggcagtc ggccgcccac atcaaggact
      361 tccttcaggc cggcaaggcc gccgcgcccg cccctgccgg caggccgccc ggcgtgcgcg
      421 gcgagtgcgt ggacgaggac ggccgcatcc tgggcatcgc caccctgcac cgcacgccga
      481 tgctgccgcc cggctcgttc gcggccgccg cgggcgtggc cagccgcgag gaacgcgagg
      541 aggcctccgc ccagctgtgc gccgaattca tgcgccatct gagcgcgctg ggcgaggccg
      601 acggcgtttc gcgcatcgac tgggtgatgg tgcgcgccac gggtaccgcg gacggcgccc
      661 gctcgcacct gggcgccacg cgggtcgggc cggtggcctt gaagccgttc gcgccgggcg
      721 caggcgaggc ggcggccgag gactgaaagc ggcgcgccgc acggcgccgg ttcactgcag
      781 cgaggatttg ggcggcagcc cggacggcag cgtgaagacc tcgatgccct cgtcggccag
      841 cgcctcgcgc tcctcggcgc tcgcctggcc gcggatggcc ttggcctcgc tctcgccgta
      901 atggatgcgg cgcgcctcct cggcgaagcg cgagccgacg tcctcggtgc tgctcaagag
      961 ctcggccacc gcctcgacga agcgccggac cgccccaccc tgcagcgcca tgtcgacggg
     1021 cggagcggcc ggctcgttgc cggcggcctc gcccgcggcc acgcgcgcgg gcgtcggcgc
     1081 gcgctcgcgc gcccccgaca ggttcaggcg cggcgcactc ggcaggcgca ccacctcggc
     1141 gttgccgcac acggggcaat cgagcatgcc gcgctcgcgc tgcgagccgt agtcgtcctc
     1201 ggacgcgaac cagccctcga aggcatggcc ggtggcacag cgcaggtcga gcaccttcat
     1261 ggccgcggct tcactcgacc gactgccagg tcggttccca ctggccgctg cgccactgct
     1321 gcagcttgag cagcgcgacc atcgtcttgg cgtcggtcag ctctccgctg gccgactgcg
     1381 cgagcagcgc ggcctcgggc atcgtgaaca cctcgaggaa ctcgccggcg tccagttgct
     1441 gcgcgccggc gaccaggccg cgcgcgaacc agatctcgat gaactcgttg gagtacgccg
     1501 gcgcattgtg caggcggccg gcataggccc actcgcgggc ggtgtagccg gtctcctcga
     1561 gcagctcgcg cctggcgcag gccagcgtcg tctcgttcgg gtcgagcttg ccggcgggaa
     1621 actccagcag cacgtggtcg agcggatagc ggtgctggcg ctcgacgacc agctggccgt
     1681 cgtcggcgat gggcacgatc atgaccgcgc cgggatgcac gatgtgctcg cgccaggcca
     1741 gctgcccgtc gggcagtttc acctggtcgc ggcgcacctt caggaagtgg ccgtcgtaga
     1801 cgttttcgga gcgcacgagg cgctccacga gatgggcgtc gacgccctcc tgcgcgcgga
     1861 tcggagagcc accggcgacg atggcctcga cgttcttctc cgtcacgcgc ggctccggct
     1921 aggagttcag cgccttcagg acggcgtcgc ggcagacgct catcaggccg tcgggcagca
     1981 ggcccaggcc cagcgtcgcg atgccgctga gggccagcag cacggtggcg ccggtctcgc
     2041 cgcgcaccgg gctcgggtcg accggctcgt cgaagaacat cgtcttgacg acgcgcaggt
     2101 agtagtacgc gccgaccagc gacagcagca ccgcgatcac ggccagcacg atgtaggtct
     2161 ggttgcccgt gctcacgagc gcctgcagca cggcgaactt cgcgtagaag ccgaccagcg
     2221 gcgggatgcc ggcgagcgag aacatgaaca gcgacatcac gccggcgatc cacggcgaac
     2281 gcttgcccag gccggccagg tcggcgatct cctcgctctc gaagccgttg cgcgccagga
     2341 ccatgatcag gccgaaggtg cccagcgtcg tcaggacgta ggtgatgatg tagaacagcg
     2401 ccgagctgta ggcgttcgcg gcggccaccg tcttcatgtc gacgacggtc ggcgtgaagc
     2461 ccagcagcat gaagcccagt tgcgcgatcg ccgagtaggc gagcaggcgc ttgaggttgg
     2521 actgcgcgat ggcggccagg ttgcccagcg tcatcgacac caccgacagc acgatcagca
     2581 tctcttccca gtccttggcc tggcccgaca ggccctcgac caggatgcgg aaggtgatgc
     2641 cgaacgccgc cagcttgggc gcgccggaga tcagcagcgt gacggccgtg ggcgcgccct
     2701 ggtagacgtc gggcacccac atgtggaacg gggcgaggcc gagcttgaac gcgaggccgg
     2761 caacgatgaa cacgaggccg aacacgagca cctgcgggtt cacggtgccc ttgtgcaggg
     2821 cctcgagcac gttggggatg tcgagcgtgc cggcggcgcc gtacatcatc gacaggccgt
     2881 acagcaggaa gccgctggcc agcgcgccga ggatgaagta cttcatcgcg gcttcggtcg
     2941 acacggcgtg gtcgcggcgc agcgcggtca gcgcgtacag cgacagcgtc atcagctcca
     3001 ggccgatgta gacgaccagg aagttgttgg ccgagaccat cacggacacg cccagcagcg
     3061 agaacatcgc cagcgacagg aactcgccct tgagcaggtc gcgcgacttc aggtaggggc
     3121 gcgcgaagcc cagcgtgatc atcgtggcga ggccggccgc caggcccagc aggtggccca
     3181 gcgggtcgat cacgaccatg cgctgcatgc cgtacagggc acggccggcc tgcagctcgt
     3241 acaggtgcat cagcgagaag gccagcagcg agatctgggc gatccagtac gtcaggccca
     3301 ggcgcttgtc ggaggcgttg acgccgacca g
//
LOCUS       DATCRO010000144         5797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1002943, whole genome shotgun sequence.
ACCESSION   DATCRO010000144 DATCRO010000000
VERSION     DATCRO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002943"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VIN75_04000"
     CDS             complement(<1..196)
                     /locus_tag="VIN75_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973355.1"
                     /translation="MITKRSLASTLAIVALGLLAACAAPVAPHAAASAAADQGDVAVI
                     HAQGPGALGRRVQHVAEIDAA"
     gene            complement(297..2948)
                     /locus_tag="VIN75_04005"
     CDS             complement(297..2948)
                     /locus_tag="VIN75_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973356.1"
                     /translation="MNKTLYTTLALALLSQWPQLAAAQSATSPPGAPAKAASAAQGQA
                     EEPAVDKVIVTANRWIETLQSVSGVVQTIDADTLRKDGITEIRSLQNEIPGMNIANQE
                     GNVEIYIRGVGSSNNTELGDPAAAPHINGIYIPRPRGLGTMFYDLERVEVNKGPQGTL
                     YGRNAMAGTLNIITAKPKLDRFGGYMQFEGGDRSSGAEAALNMPLSPTLALRAAAFMT
                     DKTSGFRNASPDPASHALTPAGEEHNQGARLSALLVPTDRMQVSMMADYGHEGGTGYP
                     GANIYTAVKATGLRAEQLPLRDIVYRGPQGTMHNDLSGVQGKASYDFDSFGAELSLSQ
                     RKVDFYQRNAQSEGIGWPGRDYSGVQWDNFSTQYWQTKSDSKVAELRLYSGEEAKVFQ
                     WNVGAFTFNEDQKVGYLSLADRGYCCFSGTEFTMPKVIGKSAALFGDGTLSVTQNLRL
                     IGGLRETEEAKSRYGIGGNIALTLGGANFACCMATRLGTEGFVPALLDRPNFDMSKVT
                     TPQQMAQFLMQTSLVPGARDTLNAQVGPIANGTNPMGNCFVRSDIDNGFVTCPTDQNG
                     GFSYANLSVPEQQVGSSKFHYTDWRLGTEYDLTKDNMVYVKVSTGHKAGGFNDSFHGS
                     TVPELFRPEQLRVLEAGSRNAFDLGGRRAVFNLSGFYYDYKDQVFQDLTCITVDTTKN
                     PPTCSGYSLVNRNIGKSHLAGLEAEGRFGLPMGFKLDLNGALLHTRIDRGVVADVRAI
                     DYGNGGKSPLIDLTGNELPLASKVNLSARLQQVFGLFGGKADWQALLNWRSAYYLTQF
                     NEKAVIDNSGNSQTALQAGFPDRQIGFLTLNLGAGYNFPGGLRLEAYVNNATDKQASQ
                     KALVGASLDIRFMNDARSYGARARMDF"
     gene            complement(3067..4161)
                     /gene="ugpC"
                     /locus_tag="VIN75_04010"
     CDS             complement(3067..4161)
                     /gene="ugpC"
                     /locus_tag="VIN75_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8973357.1"
                     /translation="MADLRLRGVRKTFGGTTEVLRGIDLDIADGEFMVFVGPSGCGKS
                     TLLRLIAGLEEATDGELRIGGERMDGVPPADRGVAMVFQSYALYPHMTVAQNLGFALK
                     MAGRPRAEIAAAVGRAAEILQIVPLLDRKPKALSGGQRQRVAIGRAIVREPRVFLFDE
                     PLSNLDAALRVQMRLELARLHRELGTTMIYVTHDQVEAMTLGDRIALFNGGRIEQVGA
                     PLALYEHPQSRFVAGFLGQPRMNFVPVGQLDAAARVLFTGRANPLPAGWMLGVRPDHL
                     RLHREGAGLPGRVERLEHVGDAVLAWIQPASGAATLAVRLAPGDALPERDAQVSLRPD
                     AGRVHAFDADGFARPLPAVAASAMEAAATP"
     gene            complement(4154..5521)
                     /locus_tag="VIN75_04015"
     CDS             complement(4154..5521)
                     /locus_tag="VIN75_04015"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8973358.1"
                     /translation="MSDLNIPLTLSPQTRATLSRDFTWGVATSAYQIEGGATAGGRGP
                     SIWDTFSHTPGKTVRGETGDVACDHYHRWPEDVALMASLGLDAYRFSISWSRVQPQGH
                     GAWNEQGLAFYDRLVDGLLAAGLQAHATLYHWDLPQGLQDRGGWASRDTALRFAEYAE
                     RIARRLGDRLASISTHNEPWCTAVLGHERGQFAPGFRDAALAADVSHHLLLSHGLALQ
                     SMRAAGCGAPMGIVLNQSSASAATDSAADRALAATEYASFVRWYMEPIFAGGYPQDAG
                     IGHYPRNIEPGDFAIIGQPLDFLGVNYYTRIWASAATPPVPAPSALGVTDMGWEIHPE
                     GLTELLVDLHRRYPGLPPIFVMENGMANGGDAVVDGRVADAPRIDYMRRHLEALARAR
                     EAGVDLRGFFYWSLMDNYEWNSGYDKRFGLVHVDYDTQVRTLKDSALWYRDTVAALRR
                     ESAHG"
     gene            complement(5615..>5797)
                     /locus_tag="VIN75_04020"
     CDS             complement(5615..>5797)
                     /locus_tag="VIN75_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8973359.1"
                     /translation="MRDMDRYTLPLALRSLQGSGQTPWGALCAGAAIAVLPLLVMFAL
                     ASRRLIDGLTAGAVKH"
BASE COUNT          817 a         1943 c         2141 g          896 t
ORIGIN      
        1 gcgcggcgtc aatttcggca acatgctgga cgcgccgacc gagggcgcct gggccctgcg
       61 cgtggatgac cgctacatcg ccctggtcgg cggcggcaga ggccgccgca tgcggggcga
      121 cgggcgctgc gcaggcggcc agcaggccca gcgcgacgat ggcgagtgtc gaggcgagcg
      181 agcgcttcgt gatcatgagg tcctcttcga aaggaaaagt gcccgcggcg tcgggggccg
      241 acggccgcgg gcacgggatc atcgcaggcg gatgcgtctg cgaagtcggg ccagggtcag
      301 aagtccatcc gggcgcgcgc gccgtacgag cgggcgtcgt tcatgaagcg gatgtcgagc
      361 gacgcgccga ccagcgcctt ctgcgaggcc tgcttgtcgg tggcgttgtt gacgtacgcc
      421 tcgagccgca gcccgcccgg gaagttgtag ccggcaccca ggttcagcgt caggaagccg
      481 atctggcggt ccggaaagcc ggcctgcagc gcggtctgcg agttgccgct gttgtcgatg
      541 acggccttct cgttgaactg cgtcaggtag tacgccgagc gccagttcag cagcgcctgc
      601 cagtcggcct tgccgccgaa caggccgaac acctgctgca ggcgggccga caggttgacc
      661 ttggaggcca gcggcagctc gttgcccgtc aggtcgatca gcgggctctt gccgccgttg
      721 ccgtagtcga tcgcgcgcac gtcggcgacg acgccgcggt cgatgcgggt gtgcaggagc
      781 gcgccgttca ggtcgagctt gaagcccatc ggcagaccga agcggccttc ggcctccagg
      841 cccgccaggt gcgacttgcc gatgttgcgg ttgaccagcg agtagccgct gcaggtgggc
      901 gggttcttcg tcgtgtcgac cgtgatgcag gtcaggtcct ggaagacctg gtccttgtag
      961 tcgtagtaga agcccgacag gttgaacacc gcgcggcggc cgccgaggtc gaacgcgttg
     1021 cgcgagccgg cctccagcac gcgcaactgc tcggggcgga acagctcggg cacggtcgag
     1081 ccgtggaagc tgtcgttgaa gccgcccgcc ttgtggcccg tcgacacctt cacgtagacc
     1141 atgttgtcct tggtgaggtc gtactcggtg cccaggcgcc agtcggtgta gtggaacttg
     1201 ctgctgccga cctgttgctc cggcacgctg aggttcgcgt agctgaagcc gccgttctgg
     1261 tcggtgggac aggtgacgaa gccgttgtcg atgtccgagc gcacgaagca gttgcccatc
     1321 gggttcgtgc cgttggcgat cgggccgacc tgcgcgttga gcgtgtcgcg cgcaccgggc
     1381 accagcgagg tctgcatcag gaactgcgcc atctgctgcg gcgtggtcac cttgctcatg
     1441 tcgaagttcg ggcgatcgag cagcgcgggc acgaagccct cggtgcccag gcgcgtggcc
     1501 atgcagcagg cgaagttggc gccgcccagc gtcagcgcga tgttgccgcc gatgccgtag
     1561 cgcgacttgg cctcctcggt ctcgcgcagg ccgccgatca ggcgcaggtt ctgcgtcacc
     1621 gacagcgtgc cgtcgccgaa cagcgcggcg gacttgccga tcaccttggg catcgtgaac
     1681 tcggtgcccg agaagcagca gtagccgcgg tcagccagcg agagatagcc caccttctgg
     1741 tcctcgttga aggtgaacgc gcccacgttc cactggaaca ccttggcctc ctcgcccgag
     1801 tacaggcgca gctcggcgac cttgctgtcg gacttggtct gccagtactg cgtcgagaag
     1861 ttgtcccatt gcacgcccga atagtcgcga ccgggccagc cgatgccctc ggattgcgca
     1921 ttgcgctggt agaagtcgac cttgcgctgg ctcagcgaca gttcggcacc gaagctgtcg
     1981 aagtcgtacg aggccttgcc ctgcacgccc gacaggtcgt tgtgcatggt gccctgcggc
     2041 ccgcggtaca cgatgtcgcg cagcggcagc tgctcggcgc gcaggccggt ggccttgacc
     2101 gcggtgtaga tgttggcgcc ggggtagccg gtgccgccct cgtggccgta gtcggccatc
     2161 atcgagacct gcatccggtc ggtcggcacc agcagcgcgg acaggcgggc gccctggttg
     2221 tgctcctcgc ccgcgggcgt gagcgcgtgc gacgccggat cgggcgaggc gttgcggaag
     2281 cccgaggtct tgtcggtcat gaaggccgcg gcgcgcagcg cgagcgtcgg cgacagcggc
     2341 atgttcagcg cggcctcggc gccgctgctg cggtcgccgc cctcgaactg catgtagccg
     2401 ccgaaacggt ccagcttcgg cttggccgtg atgatgttga gcgtgccggc catcgcgttg
     2461 cggccgtaca gcgtgccttg cgggcccttg ttcacctcga cgcgctcgag gtcgtagaac
     2521 atcgtgccca ggccgcgcgg acgcgggatg tagatgccgt tgatgtgcgg cgccgcggcc
     2581 gggtcgccca gctcggtgtt gttcgacgag cccacgccgc ggatgtagat ctcgacgttg
     2641 ccttcctggt tggcgatgtt catccccggg atctcgttct gcagcgagcg gatctcggtg
     2701 atgccgtcct tgcgcagcgt gtcggcgtcg atcgtctgca ccacgcccga gacggactgc
     2761 agcgtctcga tccagcggtt ggcggtgacg atcaccttgt cgacggcagg ctcctcggcc
     2821 tggccttgtg cggcggaggc ggccttcgcg ggcgcgccgg ggggactcgt ggcggactgc
     2881 gccgccgcga gctgcggcca ctggctcaag agcgccaggg ccagggtcgt gtacagggtc
     2941 ttgttcatgg tctcctcacc aaagtggata cggtgggcgg tctgttgtca tgcccgggaa
     3001 aacgctggag gggcgtccgg gggatcgggg cacgagcccg ccaactcctc gtgccttcat
     3061 gcgtgatcag ggtgtcgcgg cggcctccat cgcggatgcc gcaacggcgg gcagcgggcg
     3121 ggcgaacccg tccgcatcga aggcatgcac gcggccggcg tccggccgca ggctgacctg
     3181 cgcgtcgcgc tcgggcaacg cgtcgcccgg cgcgaggcgc accgcgagcg tcgcggcgcc
     3241 cgacgcgggc tggatccagg cgagcaccgc gtcgccgacg tgctccaggc gctcgacgcg
     3301 gccgggcagg cccgcgccct cgcggtgcag acgcaggtgg tcgggccgca cgcccagcat
     3361 ccagccggcg ggcagcgggt tcgcgcggcc ggtgaacagc acgcgggccg cggcgtcgag
     3421 ctggccgacg ggcacgaagt tcatgcgcgg ctggccgagg aagccggcga cgaagcgcga
     3481 ctgcgggtgc tcgtacagcg ccagcggcgc gccgacctgc tcgatgcggc cgccgttgaa
     3541 cagcgcgatg cggtcgccca gcgtcatcgc ctcgacctgg tcgtgcgtga cgtagatcat
     3601 ggtggtgccc agctcgcggt gcagccgcgc cagttccagc cgcatctgca cgcgcagcgc
     3661 ggcatcgagg ttggacagcg gctcgtcgaa caggaacacg cgcggctcgc gcacgatcgc
     3721 gcgcccgatg gccacgcgct ggcgctggcc gccggacagc gccttgggct tgcgatcgag
     3781 cagcggcacg atctgcagga tctccgcggc gcggcccacc gcggcggcga tctcggccct
     3841 gggccggccg gccatcttca gcgcgaaccc caggttctgc gccacggtca tgtgcgggta
     3901 cagcgcgtag ctctggaaca ccatcgccac gccgcggtcg gcgggcggca cgccgtccat
     3961 gcgctcgccg ccgatgcgca gctcgccgtc ggtggcctcc tcgaggcccg cgatcaggcg
     4021 cagcagcgtg gacttgccgc agcccgaggg cccgacgaac accatgaact cgccgtcggc
     4081 gatgtccagg tcgatgccgc gcaggacttc cgtcgtgccg ccgaaggtct tgcgcacgcc
     4141 gcgcaggcgc aggtcagcca tgggccgact cccggcgcag cgcggccacg gtgtcgcggt
     4201 accacagcgc gctgtccttg agcgtgcgca cctgcgtgtc gtagtcgacg tgcacgaggc
     4261 cgaagcgctt gtcgtagccc gagttccact cgtagttgtc catcaggctc cagtagaaga
     4321 agccgcgcag gtcgacgccc gcctcgcgcg cgcgggccag cgcctcgagg tggcgccgca
     4381 tgtagtcgat gcgcggggcg tctgcgacgc ggccgtccac caccgcgtcg ccaccgttgg
     4441 ccatcccgtt ttccatcacg aagatgggcg gcagcccggg gtagcggcgg tgcaggtcga
     4501 cgagcagctc ggtcaggccc tcggggtgga tctcccagcc catgtcggtg acgccgagcg
     4561 cgcttggcgc gggcaccggg ggcgtcgccg cgctggccca gatgcgcgtg tagtagttga
     4621 cgccgaggaa gtccagcggc tggccgatga tcgcgaagtc gcctggctcg atgttgcgcg
     4681 ggtagtgccc gatgcccgcg tcctgcggat agccgcccgc gaagatcggc tccatgtacc
     4741 agcgcacgaa gctcgcgtac tcggtggcgg cgagcgcgcg gtcggcggcg ctgtcggtgg
     4801 cggcgctggc ggaagactgg ttcagcacga tccccatcgg cgccccgcag ccggccgcac
     4861 gcatggactg cagcgccagg ccgtgcgaga gcagcaggtg gtgcgacacg tcagccgcca
     4921 gcgcggcgtc ccggaagccc ggcgcgaact ggccgcgctc atggcccagc accgcggtgc
     4981 accagggttc gttgtgggtg gagatcgatg cgaggcggtc gcccaggcgg cgcgcgatgc
     5041 gctcggcgta ctcggcaaag cgcagcgccg tgtcgcgcga ggcccagccg ccgcggtcct
     5101 gcagcccctg cgggaggtcc cagtggtaca gcgtggcgtg tgcctgcagg cccgcggcga
     5161 gcaggccgtc gaccaggcgg tcgtagaacg ccaggccttg ctcgttccac gcgccgtggc
     5221 cctgcggctg cacgcgcgac cacgagatcg agaagcggta ggcgtccagg cccagcgagg
     5281 ccatcagcgc gacgtcctcc ggccagcggt ggtagtggtc gcaggcgacg tcgccggtct
     5341 cgccgcgcac cgtcttgccg ggcgtgtgcg agaaggtgtc ccagatcgac ggcccgcggc
     5401 cgccggccgt ggcgccgccc tcgatctggt aggcgctggt ggccacgccc caggtgaagt
     5461 cgcgcgacag cgtggcgcgg gtctgcggcg agagcgtgag cggaatgttc aggtcggaca
     5521 tcggaatcgt cgttttcgaa gcgttgaaat cgttatcaag gcgaatcttg aaaaggccgc
     5581 gagcgcgcgc ggccggccgg aatgggtgcg aggctcagtg cttgacggct cccgcagtga
     5641 ggccgtcgat caggcggcgc gaggcgagcg cgaacatgac cagcagcggc agcaccgcga
     5701 tggccgcccc ggcgcacagc gcgccccacg gcgtctggcc cgagccctgc agcgagcgca
     5761 gcgccagcgg cagcgtgtag cggtccatgt cgcgcat
//
LOCUS       DATCRO010000145        21707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_69023, whole genome shotgun sequence.
ACCESSION   DATCRO010000145 DATCRO010000000
VERSION     DATCRO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69023"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="hutF"
                     /locus_tag="VIN75_04025"
     CDS             complement(<1..1287)
                     /gene="hutF"
                     /locus_tag="VIN75_04025"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY8973360.1"
                     /translation="MTAQRCWAPAAWMDGGWRESVVLEIDARGHWSRVEAGVPAPDGA
                     ERLAGPVLPGLVDAHSHAFQRAFAGLAERRDGDDDDFWSWRDRMYKVANAVTPESLRA
                     IAAHLYVELLRGGYTQVCEFHYLQHQADGTPYADPLALSMALADAAGDAGIGLTLLPV
                     LYERAGFAAPALRPDQRRFRAGAADVWQRSREFERRVAAAARASLVPLNAGLAIHSVR
                     AAAAESIGELRRLAHEFDGPVHVHVAEQRAELRDCVAATGQTPVAWLASQGWLDARWQ
                     LVHATHVDAGEIAATAASGAGVVICPSTEGNLGDGFTDLPAWLAADVELAIGSDSNVT
                     RSWREELRWMEYGQRLKLERRNVCAQPRGAANAAHGRPTPPRPDTAASLFERARAGGG
                     RAAGHAAWGLVPGARADLLVVDPDDASLVGLPRARWL"
     gene            complement(1284..2087)
                     /gene="hutG"
                     /locus_tag="VIN75_04030"
     CDS             complement(1284..2087)
                     /gene="hutG"
                     /locus_tag="VIN75_04030"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEY8973361.1"
                     /translation="MIPDHPSFHLKPGTTPLLVSLPHCGELIPDELRPRYLPRALDVE
                     DTDWHLQRLYAFAAGMGASVLQPRASRFVIDLNRPSDNAPMYPGANNTELCPTRFFTG
                     DPIYVEGQAPGDAEVRRRIAQWWQPYHGAIAAELARLKAVHGHAVIFDGHSIKSELPW
                     LFEGRLPALNLGTVDGTSCAPSLRRQLADVLASQDKYPHVVDGRFKGGYITRHYGRPD
                     DDVHAIQLEMTWDCYMDETPPYAYRPDKAAQVQPLLRELLRTMRDWKPR"
     gene            complement(2124..3446)
                     /gene="hutI"
                     /locus_tag="VIN75_04035"
     CDS             complement(2124..3446)
                     /gene="hutI"
                     /locus_tag="VIN75_04035"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8973362.1"
                     /translation="MTDLLESFEPGLPAQAVAAEPPPARRVWRHARLATLAEPAGWGV
                     VERGAIVTDGARIAWVGAEADLPPLGNGGVDEHDLGGALVTPGLVDAHTHLVYGGQRA
                     HEFELRLQGASYEDIARAGGGIRSTVAATRAASEDELLRSAAKRLRTLRDGGVTTVEI
                     KSGYGLSFEDEAKCLRVARRLGETGVTVRTTSLAAHALPPEFGDRADAYIDAVCDWLP
                     QWHALGLVDAVDAFCERIAFSTAQVERVFTAAAALGLPVKLHAEQLSDSGGAQLAARF
                     KALSCDHIEWLSDAGVAAMRDAGSVAMLLPGAFHFLRETRLPPIAALREAGVPLAVST
                     DHNPGTSPTLSLPLMIHLACVDLRLTPEEAVRGATVNAARALGLADRGTLAVGQRADF
                     ALWEHEHPRELAYWFGHPACRGTVVAAHMTTPSAPSTALPPPRGPRAG"
     gene            complement(3443..4042)
                     /locus_tag="VIN75_04040"
     CDS             complement(3443..4042)
                     /locus_tag="VIN75_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY8973363.1"
                     /translation="MILLPTSSVAPVPWKNGGGVTRELLRLPADGGDDWTLRISVADI
                     AADGPFSPFPGITRWFAVLEGDGVRLTFPERTLNMRRGDAPLRFDGADAPGCALLGGP
                     TRDLNVMVRHARAQSLVTAASFLDEAEPGTSPGFGFFALQPLALHGAGALPVQMPALS
                     LAWCESPFDASHAWSVEPLDAAQEHTLPGYWIRLLPHAR"
     gene            complement(4039..4863)
                     /gene="hutC"
                     /locus_tag="VIN75_04045"
     CDS             complement(4039..4863)
                     /gene="hutC"
                     /locus_tag="VIN75_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEY8973364.1"
                     /translation="MPSSTRSRLRMSSAHPAAAAPRASQDALAAGGPAGIAMQRSAPQ
                     PPYARVKQYLKDALEAGRWAPGVQMPSEAELVAQFGVSRMTVHRALRELQDAGLITRL
                     QGVGTFAAQLNRVSSTLTINDIQEEIQRRGHRHDASVHIRRAEAAPPALAARLGLVAG
                     ATVFHTLIVHHEHGVPLQCEDRYVNPACAPEYLDVDFTQMTPTHYLLDVAPLWEAHVA
                     IESALPTAQEARLLGIARTDPCLIVVRGTVSRGVPVTLARLVHPGSRYTLESQFRP"
     gene            5039..6736
                     /gene="hutU"
                     /locus_tag="VIN75_04050"
     CDS             5039..6736
                     /gene="hutU"
                     /locus_tag="VIN75_04050"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8973365.1"
                     /translation="MPKLPPATTSLPRPVRAPRGTDITCRNWLIEAAYRMIQNNLDPE
                     VAENPDALVVYGGIGKAARNWDCFDAILESLRNLKEDETLLVQSGKPVGVFRTHTDAP
                     RVLLANSNLVPKWATWEHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEAG
                     RQHFGGDLKGRWILTSGLGGMGGAQPLAASFAGASSLVIECQQSRIDFRLKTRYLDEQ
                     ATSVDDALARMHKYAAEGKAVSVGLLGNAADIMPELARRAAAGGVRPDLVTDQTSAHD
                     LVNGYLPAGWSVEQWKAAQADHTQHAALREAAARSAARHVQAMLEFQKMGIPTVDYGN
                     NIRQVAFDQGVVDAFAFPGFVPAYVRPLFCQGKGPFRWVALSGDPEDIRKTDAKMKEL
                     FPEDAHLHRWLDMAGERIAFQGLPARICWIGLGDRDRAGLAFNEMVRNGELKAPIVIG
                     RDHLDSGSVASPNRETESMMDGSDAVSDWPLLNALLATGGGATWVSLHHGGGVGMGYS
                     QHSGIVIVCDGTKAADERLKRVLWNDPASGVMRHADAGYADAREAARRHGLNLPMIK"
     gene            6865..8415
                     /gene="hutH"
                     /locus_tag="VIN75_04055"
     CDS             6865..8415
                     /gene="hutH"
                     /locus_tag="VIN75_04055"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8973366.1"
                     /translation="MTNTLQLNPGALTLDDLQALHAGGLTLTLNPSAHAPIAASAAVV
                     QKAAAGDAPVYGVNTGFGKLANKRISQGELEALQRNLIRSHSVGVGAPLQPAVVRLML
                     ALKAASLARGFSGVRQEVVDCIIAVHNAGLVPYVPSQGSVGASGDLAPLSHMTLALMG
                     EGEMLVDGQRVPALPALEKAGIAPLTLHAKEGLALINGTQTSTALALHALISFEPVLE
                     SALVIGALTVDAARGSDGPFDPRIHELRGQPGQIDVARYYRALLAGSQIRQSHMEGDD
                     RVQDPYCLRCQPQVVGACLDQLRHAALVLVREANAVTDNPLVFAEDGVLVSGGNFHAE
                     PVALAADAMAVAIAEVGAIAERRIAMMIDAGVSRLPPFLSADAGLNSGFMIAHVTAAA
                     LASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLQAMISNVSVILGIEWLASAQG
                     VEFLRPLRSSSVLEQAHALLRSECGSMATDRYLAPDIEKATALVARGALSSVFRNLEG
                     LPTLWKPA"
     gene            complement(8597..9637)
                     /locus_tag="VIN75_04060"
     CDS             complement(8597..9637)
                     /locus_tag="VIN75_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8973367.1"
                     /translation="MVQPVLVEAWRGDIVESFHRGAYAVVDTGGAVLRQGGDIDRPVY
                     ARSAVKGLQALPLVASGAAERFGLSNEELALACASHGGQPGHVATATSMLAKAGLDAD
                     VLECGTHWPSNELAARALAASGQSPCPLHNNCSGKHSGFACLGALMAGDADRARGLLR
                     GYVEPEHVVMREISASLESATGVRLADAPRATDGCSIPTFGIPVRALARAFARLGTGA
                     GLTPERARAAARLREAVAAAPLMVAGTGRLDTRLMTHFGQRVFCKVGAEGVYCAALPE
                     LGLGIALKMDDGNTARAAEVAIVALIESLLPLDDADRAFVAPLRDVEMRNWNGRLVGR
                     LAPARDFAQGAR"
     gene            complement(9671..11254)
                     /locus_tag="VIN75_04065"
     CDS             complement(9671..11254)
                     /locus_tag="VIN75_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8973368.1"
                     /translation="MSSPARSTSLTFKNKIALLAGVSIVGIVLLSAGAAWTLRDQVIE
                     GRKAGLVMAVQSQRSIAAAYEQKAEKGEMTVEAAQKAAADALRVARFGGADGKANYFF
                     IWSTDGVGVMHPFKPEWIGKNMVGKIMDPGGLDIIAALVKGASTSADGNAFVPMKFPR
                     PGKTEPVPKLQYVATVPQWNWIIGSGLYMDDVDAEVAAVLAKGLAAALVLLAAIAAIG
                     ISIYRSVLRQIGGEPAVALEAMNGVARGDLSVQLRDPVPGSLMAGLATMIESLRDTVT
                     QIRRSTDSIATASGEIAVGNNSLASRTEQTASSLQQAAATMEQLTQTVRQTSQSASEA
                     NTLATSAAQGATNGGDVVSRVVTTMDEINASSRRITDIIGVIDGIAFQTNILALNAAV
                     EAARAGEQGRGFAVVAGEVRSLAQRSAEAAREIKSLIGASVERVEAGGHLVSEAGGTM
                     QGIVDGVHRVSDIIQEITRASAEQASGISQVNTSVSALDQMTQQNAALVEESAAAAES
                     LKDQARQLAQIVQVFQLER"
     gene            complement(11415..12038)
                     /locus_tag="VIN75_04070"
     CDS             complement(11415..12038)
                     /locus_tag="VIN75_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8973369.1"
                     /translation="MLRILGKPTSINVRKVLWLCEELALAHALEPWGAGYRETDTPEF
                     RALNPNAMVPVIVDGDAVLWESNTICRYLAAKAGREDLLPREPLARARVEQWMDWQGG
                     DFNNAWRYAYMHLVRKSPAHQDPAAVVASVASWNRHVAILDEQLARTGGFVAGPAFTL
                     ADIGIGLSVNRWFETPIERPELAQVRAYYDRLAERPPFRLHGRNGMP"
     gene            12083..12961
                     /locus_tag="VIN75_04075"
     CDS             12083..12961
                     /locus_tag="VIN75_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973370.1"
                     /translation="MPVSALAMVIIAALLHAFWNIVAKKTGGNRHFVLMGGLLLALIW
                     APAGLWLGWSEVPRWGWKQWAFVGGSGFLHLAYFKVLLKGYRVSDLTVVYPVARGTGP
                     LISSMLAVAVMGEALTVHGVLGVLGVCAGVFIIAGGPSMLRASHDPGQRARMRAGLLW
                     GAATGVLIAGYTVLDGYSVKVLLLSPVILDYFGNLVRLPFMLPTLLRDPSGFVDAWRR
                     YKWHAVVLGGVSPLAYVLVLYAVRVAPLSHVAPAREISMLFAALIGGRMLGEGHRGQR
                     VLGALLMAGGVVLLAV"
     gene            complement(12950..15040)
                     /locus_tag="VIN75_04080"
     CDS             complement(12950..15040)
                     /locus_tag="VIN75_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973371.1"
                     /translation="MVASAPPSDIPSALDSSFAPAEFSRHAATARDFDDDDAGQGAPL
                     LEALLAETARPSRLGLPGGASSVFKTAGLLALLAIALAIGGARLGLDGAWVRLFDNVH
                     WTIADIGAAWLAWIGVRDAREKGLRAEFLARRWFAAGFTSYAIGQLLWDLQIFLAWQP
                     FPAPSDPFYMMMAPCCALGMMGYLRGRVRDPQRLAAVLDTLSLAIAVVALALVIYLPE
                     RGDMSALALGVMVTYPTLLLAGACVGLMLIALLRVGRAWPVWVLTGMLAAHGGLWMEW
                     NALTLRHALSDGGLVNAAFSVCTLLGGLAVTRWRAQAAAGDRVDRFYEGALRVLPLVV
                     VVGVTFAVTLQDSIAHLSPSARWLCDAAAVVVIGLAAMRQTLQLRDRDQLIEAQRLLS
                     ERENQLSELNQHLEQRVSERTRESEQRNAELSMALQQLSMAQHELVRAEKLAGLGSLV
                     QGVAAEVSASLSNADMVAITLPAQVETLGRLLAATGDAPADERRRRQVATSVNALDES
                     HKQLGLSLGQARHVMEVLQQLAVDQTTDHRRGFDLLATVREMVELTRLAHRHDPVEII
                     VTGEPDLLLDSYPGAIGQVLNQLIENAIVHGFARTAGQGTISVRVWPSGHDAVRITVH
                     DDGAGIPPTALPQVFAPFFTTRLAQGGSGLGLTIARNMVNGILGGRIEIASPEGAGTT
                     VTMTLPRLAPRSHG"
     gene            15164..16060
                     /locus_tag="VIN75_04085"
     CDS             15164..16060
                     /locus_tag="VIN75_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY8973372.1"
                     /translation="MNLAVPLPAAAENPPRALAGVDFTSAPRRGKPIVWAWGAWQRPG
                     LLKLERFEDDLSADAFAASLRRPGPWLAAFDMPFGLPRELLANLGWPADWAGSMTHYA
                     SLSRLQIVEAFAAFCDARPAGSKFAHRACDAPAGSSSSMKWVNPPVAFMLHAGVPALR
                     EAGVDLPGLATGDPSRVALEGYPGLVARALIGRRSYKSDTKAQQTTERLIARKDLVDA
                     LEQGRWRGLRLKLTHAQRDELVADAQGDRLDAALCLLLAGWADTRPGYGRPADADPVE
                     GWIVAADGHGAEGPAPVKPRRR"
     gene            complement(16071..18146)
                     /locus_tag="VIN75_04090"
     CDS             complement(16071..18146)
                     /locus_tag="VIN75_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973373.1"
                     /translation="MTAPSPRRSLGTKLRLAILGSTLVALVLALGTSIVYDLGTWHRG
                     WIADVRAQSELLGHASADDLASGNPRGANEALAMLRLQPKMRSAAVYDAQGRLFAVWN
                     AADAMPPPAAIANIDADGAHELLVRTPILDQGRMLGTVVVRARDELDDRIASYGALAL
                     ALILIAAAGTWAVSAWLKAIVTQPLAAMTDIAHEAVRDGVLSRRAPKLGEDEVGELVD
                     AFNGLLAEIERRKSEQETAAAEKDREVAERRHVQALLQRLNEELERRVHERTAQLEES
                     NRDLIAATREAENANRAKSEFLSNMSHELRTPLNAIIGFGQLLSHEDAAGAGPERNRA
                     FVQHIVDAGNHLLTLINEILNLAQIESGKVSISLEPVELREVLDECHALTRTASAQRG
                     IRLLFPTETPMVVNADRTRLKQVLLNLLSNAVKYNREHGAVIVECMPGDQGKVRIAVQ
                     DTGAGLTPEQLRGLFQPFNRLGRDKSGIEGSGIGIALTKRLVELMGGTIGVHSTPGTG
                     STFWIDLRSAEPLKVELASVQSRLPAPGELGDGVPQVATILCVDDNRANLALLTEALS
                     LRADCVVLTAADGQAGVDMARTHSPDVILMDNNMPVMSGREAMRILREDPATAGIPII
                     AVSAAAMPGAVSAGLEQGYFRYLVKPYDLVDLTDAIDAAIDVSRSAAATRRVVDRDAV
                     PGFEPPPAA"
     gene            complement(18313..19932)
                     /locus_tag="VIN75_04095"
     CDS             complement(18313..19932)
                     /locus_tag="VIN75_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8973374.1"
                     /translation="MTLLRGLGVLAARAAAGAALAALVALASPAAWAAGLRVATAFDP
                     QTMDPHSLALQYHTRVVFQIYEPLVGRDEHYRLVPVLATGWTQVDPRTWQFKLRPGVR
                     FHDGSPFTAEDAVFSFERALAPPSQRQFVLKGVKRVRALDPLTIAVELTSPDATLPQK
                     LVLVAMMSKRWAQAHHVERAQDYNARQETFAVRNANGTGAYQLERYEPDVRTTLKAFP
                     QWWNRASPANGNVDTASFVTIRSDATRLAALQSGEVDIVLDPPYQDVERLRHDPEVAV
                     TQVDDISTDYLAFDQASAALPGVAPGPDGKPRNPFKDLRVRRAVYQAINIDLVIQKVL
                     KGLASPTGDLLSIESGGPAPAAAPRPRYDPVRARDLLREAGYPDGFDVRMDCVNVAFR
                     EHVCQAIAAMLTQVGIRTELHTSPGTQFFPMITQAKVSLAEFGFTGTTEDAWQGLNGL
                     LHTWDANGAGTFNGGRYSNPRLDVLIDAIRTELDAGKRHAMIAEALKLAGDDIAYVPL
                     YRRTLSWVMQKKVQVVMLPDDTLPVRWARVR"
     gene            complement(19997..20908)
                     /gene="rocF"
                     /locus_tag="VIN75_04100"
     CDS             complement(19997..20908)
                     /gene="rocF"
                     /locus_tag="VIN75_04100"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY8973375.1"
                     /translation="MTKVSLIGAPTDIGAGARGASMGPEAMRVAQIGPTLQAHGIEVF
                     DRGNLLGPANPWQPPVDGFRHLPEVAAWNRAVHDAVHGELAIGHLPILLGGDHCLGIG
                     SISAVARHCREKGKKLRILWLDAHADFNTAVLTPSGNIHGMPVACLCGHGPQELIEIG
                     GQVPAINPKWIRQIGIRSVDPGEKKFVHEMGLEVFDMRYIDEMGMRHTMELALATLDE
                     NTHLHVSFDVDFLDPQIAPGVGTTIPGGPTYREAQLCMEMIADTGRLASLDVMELNPA
                     LDVRNQTATLAVDLIESLFGKSTLMRK"
     gene            complement(20968..>21707)
                     /locus_tag="VIN75_04105"
     CDS             complement(20968..>21707)
                     /locus_tag="VIN75_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8973376.1"
                     /translation="QLELMKPEMVQARHGIESTIVLFGSARIKTPEAAQQQLADAMRG
                     GDAQAIRRAETAVNMSNHYAEARRFAELVTVRTRHLATPIHVVTGGGPGIMEAGNRGA
                     FESGGKSIGLNIVLPHEQQPNPYITPELCFQFHYFALRKMHFLMRAVALACFPGGFGT
                     LDELFEVLTLIQTGKSRPRPILLFGAAFWKRLIDFDLLIETGMISPEDVHLFKFVETA
                     EEGWAALKGAYAFEGAEDAPAKPAGEL"
BASE COUNT         2954 a         7602 c         7990 g         3161 t
ORIGIN      
        1 gagccagcgc gcgcgcggca gcccgaccag gctcgcgtcg tccgggtcga cgacgagcag
       61 gtcggcgcgc gcgcccggca ccaggcccca cgccgcgtgt ccggccgcgc gcccgccgcc
      121 ggcgcgggcg cgctcgaaca ggctggcggc ggtgtcgggc cgtggcggcg tcggacggcc
      181 gtgcgccgcg ttggccgcgc cacgcggttg cgcgcagacg ttgcggcgct ccagcttgag
      241 ccgctggccg tattccatcc agcgcagctc ctcgcgccag ctgcgcgtga cgttgctgtc
      301 cgagccgatc gcgagctcga catctgccgc gagccacgcc ggcaggtcgg tgaagccgtc
      361 gccgaggttg ccctcggtgc tcgggcagat gacgacgccc gcgccgctgg cggcggtggc
      421 ggcgatctcg ccggcgtcca cgtgcgtggc gtgcaccagc tgccagcggg catcgagcca
      481 gccctgcgac gcgagccagg cgaccggcgt ctgcccggtg gcggcgacgc agtcgcgcag
      541 ctcggcgcgc tgctcggcga cgtgcacgtg gaccgggccg tcgaactcgt gcgcgaggcg
      601 ccgcagttcg ccgatggact cggccgcggc cgcgcgcacc gagtggatcg ccaggcccgc
      661 gttgagcggc accagcgatg cccgcgcggc cgccgcgacg cgccgttcga actcgcggct
      721 gcgctgccag acgtcggccg cgccggcgcg gaatcggcgc tggtcgggcc gcagcgcggg
      781 cgccgcgaag ccggcgcgct cgtacagcac gggcagcagc gtcaggccga tgccggcgtc
      841 gccggccgcg tcggccagcg ccatcgacag cgccagcgga tcggcgtacg gcgtgccgtc
      901 ggcctggtgc tgcaggtagt ggaattcgca gacctgcgtg tagccgccgc gcagcagctc
      961 gacgtacagg tgcgccgcga tcgcgcgcag gctctcgggc gtgaccgcgt tggcgacctt
     1021 gtacatgcgg tcgcgccagc tccagaaatc gtcgtcgtcg ccatcgcggc gctcggccag
     1081 gcccgcgaac gcgcgctgga acgcgtggct gtgggcgtcg accaggcccg gcagcaccgg
     1141 gccggccagg cgctcggcgc cgtcgggcgc gggcacgccg gcctcgacgc gcgaccagtg
     1201 gccgcgcgcg tcgatctcca gcacgacgga ctcgcgccag ccgccgtcca tccacgcggc
     1261 gggggcccag cagcgctgcg ccgtcatcgg ggcttccagt cgcgcatcgt gcgcagcagc
     1321 tcgcgcagca gcggctgcac ctgcgcggcc ttgtcggggc ggtacgcgta cggcggcgtc
     1381 tcgtccatgt agcagtccca ggtcatctcg agctggatcg catggacgtc gtcgtcgggc
     1441 cggccgtagt ggcgcgtgat gtagccgccc ttgaagcggc cgtcgaccac gtgcgggtac
     1501 ttgtcctgcg acgcgagcac gtcggccagt tgccggcgca gcgacggggc gcagctcgtg
     1561 ccgtcgacgg tgcccaggtt gagcgccggc agccggccct cgaacagcca cggcagttcg
     1621 ctcttgatgc tgtggccgtc gaagatgacg gcatggccat gcacggcctt caggcgcgcg
     1681 agctcggcgg cgatcgcgcc gtggtacggc tgccaccact gcgcgatgcg gcgcctgacc
     1741 tccgcgtcgc cgggcgcctg gccctcgacg tagatcgggt cgccggtgaa gaagcgcgtc
     1801 gggcacagct cggtgttgtt ggcgcccggg tacatcggcg cgttgtccga cggccggttc
     1861 aggtcgatga cgaagcgcga cgcgcgcggc tgcagcacgc tggcgcccat cccggcggcg
     1921 aaggcataga ggcgttgcag gtgccagtcg gtgtcctcga cgtccagggc gcgcggcagg
     1981 tagcgcgggc gcagctcgtc ggggatcagc tcgccgcagt gcggcaggct caccagcagc
     2041 ggcgtcgtgc cgggcttgag gtggaaggag ggatggtcgg ggatcatggt gattctcatc
     2101 gcggggcgag cgacgggccg cccctagccg gcccgcggtc ccctcggggg agggagcgcc
     2161 gtactcggcg cggaaggggt ggtcatgtgt gcggcgacga cggtgccgcg gcaggcgggg
     2221 tggccgaacc agtaggcgag ctcgcgcggg tgctcgtgct cccacagcgc gaagtcggcc
     2281 cgctggccga cggcgagcgt gccgcggtcg gccaggccca gcgcgcgggc cgcgttgacg
     2341 gtggcgccgc gcacggcttc ttccggcgtg aggcgcaagt ccacgcaggc caggtggatc
     2401 atcagcggca gcgacagcgt gggcgacgtg ccggggttgt ggtcggtgga gacggccagc
     2461 ggcacgccgg cctcgcgcag cgcggcgatc ggcggcagcc tggtctcgcg caggaagtgg
     2521 aacgcgccgg gcagcagcat cgccacgctg ccggcgtcgc gcatcgcggc gacgcccgcg
     2581 tcggacagcc actcgatgtg gtcgcacgac agcgccttga agcgcgccgc gagctgcgcg
     2641 ccgccggagt cgctcaattg ctccgcgtgc agcttgacgg gcaggccgag cgcggcggcc
     2701 gccgtgaaga cgcgctcgac ctgcgccgtg ctgaacgcga tgcgctcgca gaatgcgtcg
     2761 accgcgtcga ccaggcccag cgcgtgccat tgcggcagcc agtcgcagac ggcgtcgatg
     2821 taggcgtcgg cgcggtcgcc gaactcgggc ggcagcgcgt gcgcggcgag gctggtggtg
     2881 cgcacggtca cgcccgtctc gcccaggcgg cgcgcgacgc gcaggcactt ggcctcgtcc
     2941 tcgaacgaca ggccgtagcc ggacttgatc tccaccgtgg tgacgccgcc gtcgcgcagc
     3001 gtgcgcaggc gtttcgcggc gctgcgcaag agctcgtcct cgctcgcggc acgcgtggcc
     3061 gcgacggtgg agcggatgcc gccgccggcg cgcgcgatgt cctcgtagct cgcgccctgc
     3121 agccgcagct cgaactcgtg cgcccgctgt ccgccgtaga cgaggtgggt gtgcgcatcg
     3181 accaggccgg gcgtgacgag cgcgccgccg aggtcgtgct cgtcgacgcc gccgttgccg
     3241 agcggcggca ggtcggcctc ggcgccgacc caggcgatgc gcgcgccatc ggtgacgatc
     3301 gcgccgcgtt cgacgacgcc ccagccggcg ggctcggcca gcgtggccag gcgcgcatgg
     3361 cgccagacgc ggcgcgcggg cggcggctcg gcggccacgg cctgggcggg caggcccggc
     3421 tcgaaggact ccagcaggtc ggtcatcgcg cgtggggcag caggcggatc cagtagccgg
     3481 gcagggtgtg ttcctgcgcg gcgtccagcg gttcgaccga ccacgcgtgc gaggcgtcga
     3541 acggcgattc gcaccaggcc agcgacagcg caggcatctg caccggcagc gcgccggcgc
     3601 cgtgcagcgc gagcggctgc agcgcgaaga agccgaagcc ggggctggtg ccgggctcgg
     3661 cctcgtccag gaagctggcg gcggtcacga gcgactgcgc gcgcgcatgg cgcaccatca
     3721 cgttgaggtc gcgcgtgggg ccgcccagca gcgcgcagcc gggtgcgtcg gcgccgtcga
     3781 agcgcagcgg cgcgtcgccg cgccgcatgt tgagcgtgcg ttcgggaaac gtgaggcgca
     3841 cgccgtcgcc ctcgagcacc gcgaaccagc gcgtgatgcc ggggaacggc gagaacgggc
     3901 cgtcggcggc gatgtcggcc acgctgatgc gcagcgtcca gtcgtcaccg ccgtccgcgg
     3961 gcaggcgcag cagctcgcgg gtgacgccgc cgccgttctt ccacggcacc ggcgcgacgc
     4021 tggacgtggg cagaaggatc atggacggaa ctggctctcg agcgtgtagc gcgaacccgg
     4081 atgcaccagg cgcgccagcg tgaccggcac gccgcggctg acggtgccgc gcacgacgat
     4141 caggcacggg tcggtgcgcg cgatgcccag caggcgcgcc tcctgcgcgg tgggcagcgc
     4201 ggactcgatg gccacgtgcg cctcccacag cggcgcgacg tccagcaggt agtgcgtggg
     4261 cgtcatctgc gtgaagtcga cgtccaggta ctcgggcgcg caggccgggt tgacgtagcg
     4321 gtcctcgcac tgcagcggca cgccgtgctc gtggtgcacg atcagcgtgt ggaacacggt
     4381 ggcgcccgcc accaggccca gccgcgccgc gagcgcgggc ggcgcggcct cggcgcgccg
     4441 gatgtgcacg ctcgcatcgt ggcggtggcc gcggcgctgg atctcttcct ggatgtcgtt
     4501 gatcgtcagc gtggacgaca cgcggttgag ctgcgccgcg aaggtgccca cgccctgcag
     4561 ccgcgtgatc aggccggcgt cctgcagctc gcgcagcgcg cggtgcacgg tcatgcggct
     4621 gacgccgaac tgcgcgacga gttcggcctc cgacggcatc tgcacgcccg gcgcccagcg
     4681 gcccgcttcc agcgcgtcct tcaggtattg cttgactcgc gcgtagggcg gttgcggcgc
     4741 cgagcgctgc atggcgatgc cggcgggccc tccggcggcg agggcgtcct gcgaggcgcg
     4801 cggggccgcg gcggcgggat gggcggacga catgcgaagt cgggaacggg tggacgacgg
     4861 catggggcga tcctacttgc acaaggttgt ctagacaagt agagtacgac ccgtatcgac
     4921 gatgccctcc gtgtgcggat tcgcaccgaa agcaggggcc agacccgccc ggcgcgccgg
     4981 ccataccctg tttgaggtat gtcgcacgcc acctccccgg agaccttccg tgaacgacat
     5041 gcccaagctc ccgcctgcca cgacgtcgct gccgcgcccg gtgcgcgcac cgcgtggtac
     5101 cgatatcacg tgccgcaact ggctcatcga ggccgcgtac cggatgatcc agaacaacct
     5161 cgatccggag gtcgccgaga accccgacgc gctggttgtc tatggcggta tcggcaaggc
     5221 cgcgcgcaac tgggactgct tcgacgcgat cctggagtcg ctgcgcaacc tcaaggaaga
     5281 cgagacgctg ctggtccagt cgggcaagcc ggtcggcgtg ttccgcacgc acaccgacgc
     5341 gccgcgcgtg ctgctggcca actcgaacct ggtgcccaag tgggcgacct gggaacactt
     5401 caacgagctc gaccgcaagg gcctgatgat gtacggccag atgacggccg gctcgtggat
     5461 ctacatcggc agccagggca tcgtgcaggg cacctacgag accttcgtgg aggccggccg
     5521 ccagcatttc ggcggcgacc tgaagggccg ctggatcctc acgtcgggcc tgggcggcat
     5581 gggcggcgcg cagccgctgg ccgccagctt cgcgggcgcc agctcgctgg tcatcgagtg
     5641 ccagcagagc cgcatcgact tccgcctgaa gacgcgctac ctggacgagc aggcgacgtc
     5701 ggtcgacgac gcgctggcgc ggatgcacaa gtacgcagcc gagggcaagg cggtctccgt
     5761 cggcctgctg ggcaacgccg cggacatcat gccggagctc gcgcgccgcg cggccgccgg
     5821 cggcgtgcgc cccgacctcg tcaccgacca gacctcggcg cacgacctcg tcaacggcta
     5881 cctgcccgcg ggctggagcg tcgagcagtg gaaggccgcg caggccgacc acacgcagca
     5941 cgccgcgctg cgcgaggccg cggcccgcag cgccgcgcgc cacgtgcagg cgatgctcga
     6001 gttccagaag atgggcatcc ccacggtcga ctacggcaac aacatccgcc aggtcgcgtt
     6061 cgaccagggc gtggtcgacg cgttcgcgtt cccgggcttc gtgccggcct acgtgcggcc
     6121 gctgttctgc cagggcaagg gcccgttccg ctgggtggcg ctgtcgggcg atccggagga
     6181 catccgcaag accgacgcga agatgaagga gctgttcccc gaggacgcgc acctgcaccg
     6241 ctggctggac atggccggcg agcgcatcgc cttccagggc ctgcccgcgc gcatctgctg
     6301 gatcggcctg ggcgaccgcg accgcgcggg cctggcattc aacgagatgg tgcgcaatgg
     6361 cgaactcaag gcgcccatcg tcatcggccg cgaccacctc gactcgggct ccgtcgcctc
     6421 gcccaaccgc gagacggaga gcatgatgga cggctcggac gcggtgtccg actggccgct
     6481 gctcaacgcg ctgctggcca cgggcggcgg cgcgacgtgg gtgtcgctgc accacggcgg
     6541 cggcgtgggc atgggctact cgcagcactc gggcatcgtg atcgtctgcg acggcacgaa
     6601 ggcggccgac gagcgcctga agcgcgtgct gtggaacgac ccggcctccg gcgtgatgcg
     6661 ccacgccgac gccggctacg ccgatgcccg cgaggcggcc aggcgccacg gcctcaacct
     6721 gccgatgatc aagtaatccg ttggggtcag gtcttgaacg ttgcggggtc aggtcttgag
     6781 tgaacgattc aagacctgac cccgcaacgt tcaagacctg accccgcgat gacgcaacct
     6841 gccgatgatc aagtaactcc tgcgatgacg aacacgctcc aactcaatcc cggcgccctg
     6901 acgctggacg acctgcaggc cctgcacgcc ggcggcctca cgctgacgct gaatccgtcg
     6961 gcgcatgcac cgatcgcggc cagtgccgcg gtcgtgcaga aggcagcggc cggcgatgcg
     7021 ccggtctacg gcgtcaacac gggcttcggc aagctcgcca acaagcgcat cagccagggc
     7081 gagctcgagg cgctgcagcg caacctgatc cgttcgcact cggtgggcgt gggcgcgccg
     7141 ctgcagccgg cggtcgtgcg cctgatgctc gcgctcaagg ccgcgagcct ggcacgcggg
     7201 ttctccggcg tgcgccagga ggtcgtcgac tgcatcatcg ccgtgcacaa cgcgggcctg
     7261 gtgccgtacg tgccgtcgca gggctcggtg ggcgcgtcgg gcgacctggc gccgctgtcg
     7321 cacatgacgc tcgcgctgat gggcgagggc gagatgctgg tcgacggcca gcgcgtgccc
     7381 gcgctgcccg cgctggagaa ggcgggtatc gcgccgctga cgctgcacgc caaggaaggg
     7441 ctggcgctga tcaacggcac ccagacctcg acggcgttgg cgctgcacgc gctgatcagc
     7501 ttcgagccgg tgctcgagtc ggcgctggtg atcggcgcgc tgacggtgga cgcggcgcgc
     7561 ggcagcgacg gcccgttcga cccgcgcatc catgagctgc gcggccagcc cggccagatc
     7621 gacgtggccc gctactaccg cgcgctgctg gccggcagcc agatccgcca gtcgcacatg
     7681 gaaggcgacg accgcgtgca ggacccgtac tgcctgcgct gccagcccca ggtcgtcggc
     7741 gcctgcctcg accagctgcg gcacgccgcg ctggtcctgg tgcgcgaggc caacgccgtc
     7801 accgacaacc cgctcgtgtt cgccgaggac ggtgtgctcg tatccggcgg caacttccac
     7861 gccgaaccgg tggcgctcgc cgccgacgcg atggccgtgg cgatcgccga agtgggcgcg
     7921 atcgccgagc gccgcatcgc gatgatgatc gacgcgggcg tctcgcgcct gccgccgttc
     7981 ctgtcggccg acgcgggcct gaactcgggc ttcatgatcg cgcacgtgac ggcggcggcg
     8041 ctggccagcg agaacaagtc gctggcgcat ccggccagcg tcgattcgct gccgacgtcg
     8101 gccaaccagg aagaccacgt ctcgatggcg accttcgcgg cgcggcgcct gcaggcgatg
     8161 atctcgaacg tctcggtcat tctgggcatc gagtggctgg cctccgcgca gggcgtcgag
     8221 ttcctgcggc cgctgcgcag ctcgtcggtg ctggaacagg cgcatgcgct tctgcgttcc
     8281 gagtgcggtt ccatggcgac ggatcgctac ctggcgcccg acatcgagaa ggcgacggcg
     8341 ctcgtggcgc gcggggcgtt gtcgtcggtg ttcaggaatc tcgagggact gccgacgctc
     8401 tggaagccgg cctgattcgt tgcgccggat tcatcttcgg gagccgccgc gaccttggtt
     8461 gcggcggctc cttgtgcttt tgcgagacgc catcgtctcg cggcgtcagg tcttggatcg
     8521 tggcatgccg gcgccgttga agaaggtgtc aggtcttgaa acgtgcactg cacgattcaa
     8581 gacctgaccc cgcaactcag cgcgcgccct gagcgaagtc ccgcgctggc gccagacgtc
     8641 ccaccagccg cccgttccag ttgcgcatct cgacgtcgcg cagcggcgcc acgaacgcgc
     8701 ggtcggcgtc gtccagcggc agcagcgatt cgatcagcgc gacgatcgcg acctcggccg
     8761 cgcgcgccgt gttgccatcg tccatcttca acgcgatgcc cagtccgagc tcgggcagcg
     8821 ccgcgcagta gacgccctcg gcgccgacct tgcagaacac gcgctggccg aagtgcgtca
     8881 tcaggcgcgt gtccaggcgc cccgtgccgg ccaccatcag cggcgcggcg gccaccgcct
     8941 cgcgcaggcg cgcggccgcg cgggcgcgct cgggcgtgag gcccgcgccc gtgcccaggc
     9001 gcgcgaacgc gcgggcgagc gcgcgcacgg ggatgccgaa ggtggggatg gagcagccgt
     9061 cggtggcgcg cggcgcgtcg gccaggcgca cgccggtggc cgattcgagc gacgcgctga
     9121 tctcgcgcat gacgacgtgt tcgggctcca cgtagccgcg cagcaggccg cgcgcgcggt
     9181 cggcgtcgcc ggccatcagc gcgcccaggc aggcgaagcc cgaatgcttg ccggagcagt
     9241 tgttgtgcag cgggcagggc gactggccgc tggccgcgag cgcgcgcgcc gccagttcgt
     9301 tggacggcca gtgcgtgccg cactcgagca cgtcggcgtc cagaccggcc ttggcgagca
     9361 tcgaggtcgc cgtggccacg tgtccgggtt gcccgccgtg cgacgcgcag gccagcgcga
     9421 gctcctcgtt ggacagcccg aagcgttccg cggcgccgga ggccaccagc ggcagcgcct
     9481 gcagcccctt gacggccgaa cgcgcataga cgggccggtc gatgtcgccg ccctggcgca
     9541 gcacggcacc tcctgtgtcg accacggcgt acgcgccgcg atggaaggat tcgacgatgt
     9601 cgccgcgcca ggcttcgacg aggacgggtt ggaccatgcg gcactcctgc gagggaatgc
     9661 ctattttgcg tcagcgttcc aactggaaga cctgcacgat ctgggcgagc tggcgcgcct
     9721 ggtccttcaa gctctcggcg gcggccgcgg attcctcgac gagtgcggcg ttctgctgcg
     9781 tcatctggtc cagcgccgac acgctggtgt tgacctgcga gatgcccgag gcctgctcgg
     9841 cgctggcccg ggtgatctcc tggatgatgt cgctgacgcg gtgcacgccg tcgacgatgc
     9901 cctgcatcgt gccgccggcc tcgctcacca ggtggccgcc ggcttcgacg cgctcgaccg
     9961 aggcgccgat cagcgacttg atctcgcgcg cggcctcggc cgagcgctga gccagcgagc
    10021 gcacctcgcc ggccacgacc gcgaatccgc ggccctgttc gcctgcgcgc gcggcctcga
    10081 cggctgcgtt cagtgccagg atgttggtct ggaacgcgat gccgtcgatc acgccgatga
    10141 tgtcggtgat cctgcgcgag ctcgcgttga tctcgtccat cgtggtgacc acgcgcgaga
    10201 cgacgtcgcc gccgttggtc gcgccctgtg cggccgatgt ggcgagcgta ttggcctccg
    10261 aggccgattg cgaggtctgg cgcacggtct gcgtcagctg ttccatcgtg gccgcggcct
    10321 gctgcaggct ggacgcggtc tgctcggtgc ggctcgcgag gctgttgttg cccaccgcga
    10381 tctcgcccga ggcagtggcg atgctgtcgg tcgatcgacg gatttgcgtc acggtgtcgc
    10441 gcagcgactc gatcatcgtc gcgagtcccg ccatcaggct gcccggcacg ggatcgcgca
    10501 gctgcaccga caggtcgccg cgcgcgacgc cgttcatcgc ctcgagcgcg acggcgggct
    10561 cgccgccgat ctggcgcagc acgctgcggt agatcgagat gccgatggcc gcgatggccg
    10621 cgagcagcac gagcgccgcg gccaggccct tggcgagcac tgcggcaacc tcggcgtcga
    10681 cgtcgtccat gtacagcccc gagccgatga tccagttcca ctgcggcacc gtggccacgt
    10741 actgcagctt gggcaccggc tcggtcttgc cgggacgcgg gaacttcatc gggacaaagg
    10801 cgttgccgtc agcactggtg ctggcgccct tgacgagcgc cgcgatgatg tccaggccgc
    10861 ccggatccat gatcttgccc accatgttct tgccgatcca ttccggcttg aacggatgca
    10921 tcacgccgac gccgtcggtg ctccagatga agaagtagtt cgccttgccg tcggcgccgc
    10981 cgaagcgggc gacgcgcagc gcgtcggccg cagccttctg ggcggcctcg accgtcatct
    11041 cgcccttctc ggccttctgc tcgtaggccg ccgcgatgct gcgctgggac tgcacggcca
    11101 tcacgaggcc ggccttgcgg ccctcgatga cctggtcgcg cagcgtccat gcggcgccgg
    11161 ccgacaacag cacgatgccg acgatggaca cgcccgcgag cagggcgatc ttgttcttga
    11221 acgtgaggga ggtggagcgg gcaggagagg acatcgacgc tttctcggct gagtcacggg
    11281 ccggtcggcc ccgcgcatcc gtcgcgcagg gggacgagcg cgcgttcaca ctctaggccg
    11341 cggtccgtcg gccgaaccga ggaaaagtcg ggcaatttcc cgacagactc cttccaggcc
    11401 tcccgacgcc gcgttcaggg catgccgttg cgcccgtgca gtcggaaggg cggacgctcg
    11461 gcgaggcggt cgtagtacgc gcgcacctgc gccagctcgg gccgctcgat cggggtctcg
    11521 aaccagcggt tcaccgacag gccgatcccg atgtccgcca gcgtgaacgc cggccccgcg
    11581 acgaacccgc cggtgcgcgc aagctgctcg tccaggatgg cgacgtggcg gttccacgag
    11641 gcgacgctgg cgaccacggc ggccggatcc tggtgcgccg ggctcttgcg caccaggtgc
    11701 atgtacgcgt agcgccaggc gttgttgaag tcgccgccct gccagtccat ccattgctcg
    11761 acgcgggcgc gggccagcgg ctcgcgcggc agcaggtcct cgcgtcccgc cttggcggcc
    11821 aggtagcggc agatcgtgtt ggactcccac agcacggcgt cgccatcgac gatcaccggc
    11881 accatcgcgt tcgggttcag cgcgcggaac tccggcgtgt cggtctcgcg atagcccgcg
    11941 ccccagggct cgagcgcatg cgcgagcgcg agctcctcgc acagccacag caccttgcgc
    12001 acgttgatcg acgtcggctt gcccaggatc ctcagcatct ttcccccaag atcatccaca
    12061 gtgtggtccc tagaatcggg ccatgcccgt ttccgctctc gcgatggtca tcatcgccgc
    12121 cttgctgcac gcattctgga acatcgtcgc caagaagacc ggaggcaaca ggcatttcgt
    12181 gctgatgggc gggctgctgc tcgcgctgat ctgggcgccc gcaggcctgt ggctgggatg
    12241 gagcgaggtg ccgcgctggg gctggaagca atgggccttc gtcggcggca gcggcttcct
    12301 gcacctggcg tacttcaagg tgctgctcaa gggctaccgc gtctccgacc tgaccgtcgt
    12361 ctatcccgtg gcgcgcggca ccgggccgtt gatcagctcg atgctggcgg tcgcggtgat
    12421 gggcgaggcg ctgacggtgc acggcgtcct gggcgtgctg ggcgtgtgcg cgggcgtgtt
    12481 catcatcgcg ggcgggccgt cgatgctgcg cgcgtcgcac gatccgggtc agcgggcgcg
    12541 catgcgcgcg ggcctgctgt ggggtgcggc caccggcgtg ctgatcgccg gctacacggt
    12601 cctcgacggc tattcggtga aggtgctgct gctcagcccg gtcatcctcg actacttcgg
    12661 caacctggtg cgcctgccgt tcatgctgcc gacgctgctg cgcgacccgt ccggcttcgt
    12721 cgacgcctgg cggcgctaca agtggcacgc ggtggtgctg ggcggcgtga gcccgctcgc
    12781 ctacgtgctg gtgctgtacg cggtgcgcgt ggcgccgctg tcgcacgtgg cgccggcgcg
    12841 cgagatctcg atgctgttcg ccgcgctgat cggcggccgc atgctcggcg agggccatcg
    12901 cggccagcgc gtgctcggcg cgctgctgat ggccggcggc gtcgtcctgc tagccgtgtg
    12961 acctgggcgc cagccgcggc agcgtcatcg tgacggtcgt gcccgcgccc tcgggcgacg
    13021 cgatctcgat gcggccgccc aggatcccgt tgaccatgtt gcgcgcgatc gtcagcccca
    13081 ggccgctgcc gccctgggcc aggcgcgtgg tgaagaaggg cgcgaacacc tgcggcaggg
    13141 cggtcggcgg gatgccggcg ccgtcgtcgt gcacggtgat gcgcacggcg tcgtggcccg
    13201 agggccagac gcgcacggag atcgtgccct gcccggcggt gcgcgcgaag ccgtgcacga
    13261 tggcgttctc gatgagctgg ttgagcacct ggccgatcgc gccggggtag ctgtcgagca
    13321 gcaggtcggg ctcgccggtg acgatgatct cgaccgggtc gtggcggtgc gcgaggcgcg
    13381 tgagctccac catctcgcgc accgtggcca gcaggtcgaa gccgcggcgg tggtcggtgg
    13441 tctggtccac ggccagctgc tggagcacct ccatgacgtg gcgcgcctga ccgagcgaca
    13501 ggcccagctg cttgtggctc tcgtcgagcg cgttgacgga cgtcgccacc tgccggcggc
    13561 ggcgctcgtc ggcgggcgcg tcgccggtcg ccgcgagcag ccggcccagc gtctccacct
    13621 gcgcgggcag cgtgatggcc accatgtcgg cgttggacag cgatgccgag acttccgccg
    13681 ccacgccctg caccagcgag cccaggccgg cgagcttctc ggcgcgcacc agttcgtgct
    13741 gcgccatcga cagctgctgc agcgccatcg acagctcggc gttgcgctgc tccgactcgc
    13801 gcgtgcgctc ggacacccgc tgctccaggt gctggttcag ctcgctgagc tggttctcgc
    13861 gctcgctcag caggcgctgc gcctcgatca gctggtcgcg gtcgcgcagc tgcagcgtct
    13921 ggcgcatcgc ggccagcccg atgacgacga cggctgccgc gtcgcacagc cagcgcgccg
    13981 acggcgacag gtgcgcgatc gagtcctgca gcgtcacggc gaaggtgacg cccaccacga
    14041 cgaccagcgg cagcacgcgc agcgcgcctt cgtagaagcg gtcgacgcgg tcgccggccg
    14101 cggcctgcgc gcgccagcgc gtcaccgcca ggccgcccag cagcgtgcac acggagaacg
    14161 cggcgttgac gaggccgccg tccgacagcg cgtggcgcag cgtcagcgcg ttccattcca
    14221 tccacagtcc gccgtgcgcg gccagcatgc ccgtgagcac ccagacgggc cacgcgcggc
    14281 ccacgcgcag cagcgcgatc agcatcaggc cgacgcacgc gccggccagc agcagcgtcg
    14341 ggtacgtgac catcacgccg agcgccagcg cgctcatgtc gccgcgctcg ggcaggtaga
    14401 tcaccagcgc cagcgcgacg acggcgatcg ccagcgacag cgtgtccagc acggccgcca
    14461 ggcgctgcgg atcgcgcacg cgtccgcgca ggtagcccat catgccgagc gcgcagcacg
    14521 gcgccatcat catgtagaac ggatcggacg gtgccggaaa cggctgccac gccaggaaga
    14581 tctgcaggtc ccacagcagc tggccgatgg cgtacgacgt gaagccggcg gcgaaccagc
    14641 gccgcgccag gaactcggcg cgcaggccct tctcgcgcgc gtcgcgcacg ccgatccagg
    14701 cgagccaggc ggcgccgatg tcggcgatgg tccagtgcac gttgtcgaac aggcgcaccc
    14761 aggcgccatc cagtcccagc cgcgcgccgc cgatggccag cgcgatggcc agcagcgcca
    14821 gcaggccggc cgtcttgaac acgctgctcg cgccgccggg caggcccagg cgcgacggcc
    14881 gggccgtctc ggccaacagc gcctcgagca gcggcgcgcc ctggccggcg tcgtcgtcgt
    14941 cgaagtcgcg cgccgtcgcg gcgtggcgcg agaactcggc gggagcgaac gacgaatcga
    15001 gggcggacgg gatgtcggag ggcggcgcgg acgcgaccat ggcggccagg cggggctccg
    15061 gacggcggcc ggagcaggga gggactgcga ggaatccgag gctacctcaa gccgccccgt
    15121 gcatgggccg gcccagcctg tcttgtgagc aaaaatgcct gacatgaacc ttgccgtgcc
    15181 gcttccagcc gctgccgaga acccgccgcg ggcgctcgcg ggcgtcgatt tcacgagcgc
    15241 gccgcggcgc ggcaagccca tcgtgtgggc gtggggcgcc tggcagcggc cgggcctgct
    15301 caagctcgag cgcttcgagg acgacctgtc ggccgacgcg ttcgcggcgt cgctgcgccg
    15361 ccccggcccg tggctggccg cgttcgacat gcccttcggc ctgccgcgcg aactgctcgc
    15421 caacctcggc tggcctgccg actgggccgg cagcatgacg cactacgcga gcctgtcgcg
    15481 cctgcagatc gtggaggcct tcgcggcgtt ctgcgacgcg cgcccggcgg gctcgaaatt
    15541 cgcgcaccgc gcctgcgacg cccccgccgg aagcagcagc tcgatgaagt gggtcaaccc
    15601 gccggtggcg ttcatgctgc atgcgggcgt gccggcgctg cgcgaggcgg gcgtggatct
    15661 gccggggctg gcgacgggcg atccgtcgcg cgtcgcgctg gagggctatc cgggcctcgt
    15721 ggcgcgcgcg ctgatcggcc gccgctcgta caagagcgac accaaggcgc agcagacgac
    15781 cgagcgcctg atcgcgcgca aggatctcgt cgacgcgctg gagcagggcc gctggcgcgg
    15841 cctgcggctc aagctgacgc acgcgcagcg cgacgagctg gtggccgacg cgcagggcga
    15901 ccggctcgac gccgcgctgt gcctgctgct ggccggctgg gccgataccc ggccagggta
    15961 tggccgcccg gcagatgccg acccggtcga gggctggatc gtcgccgccg acggccacgg
    16021 cgcggaagga cccgcgcccg tgaagccgcg ccgccgctga ggcgcgcccg tcaagccgcc
    16081 ggcggcggct cgaagccggg cacggcgtcg cggtcgacga cgcgccgcgt cgcggcggcg
    16141 ctgcgcgaga cgtcgatcgc cgcatcgatg gcgtcggtca ggtcgaccag gtcgtacggc
    16201 ttgacgaggt agcggaagta gccctgctcg agaccggccg acacggcgcc gggcatcgcc
    16261 gccgcgctca ccgcgatgat cgggatgccc gcggtggcgg gatcctcgcg caggatgcgc
    16321 atcgcctcgc ggccgctcat gacgggcatg ttgttgtcca tcaggatgac gtcggggctg
    16381 tgggtgcgcg ccatgtcgac accggcctgg ccatcggcgg cggtcagcac gacgcagtcg
    16441 gcgcgcagcg acagcgcctc ggtcagcagc gcgaggttgg cgcggttgtc gtccacgcac
    16501 aggatggtag ccacctgcgg cacgccgtcc ccgagctcgc cgggcgcggg caggcgcgac
    16561 tgcacgctcg cgagttccac cttcagcggc tcggcgctgc gcaggtcgat ccagaacgtg
    16621 ctgccggtgc cgggcgtgct gtgcaccccg atcgtgccgc ccatcagctc caccagccgc
    16681 ttggtcagcg cgatgccgat gccgctgcct tcgatgccgc tcttgtcgcg cccgaggcgg
    16741 ttgaacggct ggaagagccc gcgcaactgc tcgggcgtga ggccggcgcc ggtgtcctgc
    16801 accgcgatgc gcaccttgcc ctggtcgccc ggcatgcact ccacgatgac ggcgccgtgc
    16861 tcgcggttgt acttgaccgc gttggaaagc aggttcagca gcacctgctt gagccgcgtc
    16921 cggtcggcat tgaccaccat cggcgtctcc gtcgggaaca agaggcggat gccgcgctgg
    16981 gccgacgccg tgcgggtcag cgcgtggcat tcgtcgagca cctcgcgcag ttccaccggc
    17041 tccaggctga tggagacctt gcccgactcg atctgcgcga ggttcaggat ctcgttgatc
    17101 agcgtgagca ggtggttgcc ggcgtcgacg atgtgctgca cgaacgcccg gttgcgctcg
    17161 gggccggcgc cggccgcgtc ctcatggctc agcagctggc cgaagccgat gatcgcgttg
    17221 agcggcgtgc gcagctcgtg gctcatgttc gacaggaact ccgacttggc gcggttggcg
    17281 ttctcggcct cgcgcgtggc ggcgatcagg tcgcggttgg actcctccag ctgcgcggtg
    17341 cgctcgtgca cgcggcgctc gagctcctcg ttgagccgct gcagcagcgc ctgcacgtgg
    17401 cggcgctccg cgacctcgcg gtccttctcc gccgccgcgg tctcctgttc ggacttgcgc
    17461 cgctcgatct cggccagcag gccgttgaac gcgtccacca gctcgccgac ctcgtcctcg
    17521 cccagcttgg gtgcgcggcg cgagagcacg ccgtcgcgca cggcctcgtg cgcgatgtcg
    17581 gtcatcgcgg cgagcggctg cgtcacgatg gccttgagcc acgctgagac ggcccaggtg
    17641 ccggcggccg cgatcaggat cagcgcgagc gccagcgcgc cgtagctggc gatgcggtca
    17701 tcgagctcgt cgcgcgcccg cacgacgacc gtgcccagca tgcggccctg gtcgaggatg
    17761 ggtgtgcgca ccagcagctc gtgcgcgccg tcggcgtcga tgttggcgat cgccgccggc
    17821 ggcggcatcg cgtccgcggc gttccacacg gcgaacagcc gcccctgcgc gtcgtagacc
    17881 gcggcgctgc gcatcttggg ctgcagccgc agcatggcca gcgcctcgtt ggcgccgcgc
    17941 gggttgccgc tggccaggtc gtccgcgctc gcgtggccca gcagctcgga ctgcgcccgc
    18001 acgtcggcga tccagccacg gtgccacgtc cccaggtcgt agacgatgga ggttccgagg
    18061 gccagcacga gcgccacgag ggtggagccg aggatggcga gccgcagctt ggtgccgagg
    18121 gatcggcggg gcgatggagc ggtcatgggc cggcgccagg ggcgcggtgc tacggtccag
    18181 tagcgggtag cacgcggcat acccgaatct ggtccaggat gctctttgta cgccgtgtca
    18241 catgcggatg cgaagtcgtt cacgccaaca ggggctaaac gcgctccaag gcgcgccggc
    18301 gcggcccggc ggctaccgga cgcgcgccca gcgcacgggc agcgtgtcgt ccggcagcat
    18361 gaccacctgc acttttttct gcatgaccca gctgagcgtg cgccggtaca gcggcacgta
    18421 ggcgatgtcg tcgccggcca gcttcagcgc ctcggcgatc atcgcgtgcc gcttgccggc
    18481 gtccagctcg gtgcggatgg cgtcgatcag cacatcgagc cgcggattcg aatagcggcc
    18541 gccgttgaag gtgccggctc cgttggcatc ccacgtgtgc agcagcccgt tcaggccctg
    18601 ccaggcgtcc tcggtggtcc ccgtgaagcc gaattccgcc aggctcacct tggcctgcgt
    18661 gatcatcggg aagaactggg tacccggtga ggtatgcagc tcggtgcgga tgcccacctg
    18721 cgtcagcatc gccgcgatcg cctggcagac gtgttcgcgg aaggccacgt tgacgcagtc
    18781 catgcgcacg tcgaacccat cgggatagcc cgcctcgcgc agcaggtcgc gggcgcgcac
    18841 ggggtcgtag cgcggacgcg gcgcggcggc gggtgcgggg ccgccggact cgatcgacag
    18901 caggtcgccg gtgggcgagg ccaggccctt gagcaccttc tggatgacga ggtcgatgtt
    18961 gatcgcctgg tagacggcgc gccgcacgcg caggtccttg aacgggttgc gcggcttgcc
    19021 gtccgggccg ggcgccacgc cgggcagggc ggcgctcgcc tggtcgaagg cgaggtagtc
    19081 ggtggagatg tcgtccacct gcgtgacggc cacctcaggg tcatggcgca gccgctcgac
    19141 gtcctggtac ggcgggtcga gcacgatgtc gacctcgccc gattgcagcg cggccagccg
    19201 cgtcgcgtcc gagcggatgg tgacgaagct ggcggtgtcg acgttgccgt tggcggggct
    19261 cgcacggttc caccactgcg ggaaggcctt gagcgtggtg cgcacgtcgg gctcgtagcg
    19321 ttcgagctgg taggcgccgg tgccgttggc gttgcgcacc gcgaacgtct cctggcgcgc
    19381 gttgtagtcc tgcgcccgct cgacgtggtg ggcctgcgcc cagcgcttgc tcatcatcgc
    19441 caccagcacc agcttctgcg gcagcgtggc gtcgggcgac gtcagctcga ccgcaatggt
    19501 caggggatcc agcgcacgca cccgcttgac gcccttgagc acgaactgcc gctgcgacgg
    19561 cggcgcgagc gcgcgctcga acgagaacac cgcgtcctcg gcggtgaacg ggctgccgtc
    19621 gtggaagcgc acgccggggc gcagcttgaa ctgccacgtg cgcgggtcga cctgtgtcca
    19681 tccggtggcg agcacgggca ccaggcgata gtgctcgtcg cggcccacca gcggctcgta
    19741 gatctggaac accacgcgcg tgtggtactg cagcgcgagg ctgtgcggat ccatcgtctg
    19801 cgggtcgaac gcggtggcca cgcgcaggcc ggccgcccag gcggcgggtg aggcgagcgc
    19861 cacgagcgcg gcgagcgcgg ccccggcggc ggccctcgcg gcaagcactc ccagaccgcg
    19921 cagcagcgtc atgcagcaga cctccggcga catccgaagg cccagcatac gccaggacgc
    19981 cggccggtcg gcgtgcttat ttgcgcatca gcgtgctctt gccgaacagg ctctcgatca
    20041 ggtcgacggc cagcgtggcg gtctggttgc gcacgtccag cgcggggttg agctccatga
    20101 cgtcgagcga ggccaggcgg ccggtgtcgg cgatcatctc catgcacagc tgcgcctcgc
    20161 gatacgtggg gccgccggga atggtggtgc cgacgccggg ggcgatctgc gggtcgagga
    20221 agtcgacgtc gaagctgacg tgcaggtggg tgttctcgtc gagcgtcgcg agcgccagct
    20281 ccatcgtgtg gcgcatgccc atctcgtcga tgtagcgcat gtcgaacacc tccaggccca
    20341 tctcgtgcac gaacttcttc tcgcccggat cgacgctgcg gatgccgatc tggcggatcc
    20401 acttcgggtt gatcgcgggc acctggccgc cgatctcgat gagctcctgc gggccgtggc
    20461 cgcacaggca ggccacgggc atgccgtgga tgttgccgct gggcgttagc acggcggtgt
    20521 tgaagtcggc gtgcgcgtcc agccacagga tgcgcagctt cttgcccttc tcgcggcagt
    20581 ggcgcgcgac ggcgctgatc gagccgatgc ccaggcagtg gtcgccgccc agcaggatgg
    20641 gcaggtgccc gatcgcgagc tcgccgtgca cggcgtcgtg caccgcgcgg ttccaggccg
    20701 ccacctcggg caggtgccgg aagccgtcga cgggcggctg ccacgggttg gccgggccca
    20761 gcaggttgcc gcggtcgaac acctcgatgc cgtgggcctg cagcgtcggg ccgatctggg
    20821 cgacgcgcat cgcctcgggg cccatgctgg cgccacgggc tccggcgccg atgtcggtcg
    20881 gcgcgccgat gagtgaaact ttggtcatgg cgcgattgtc cccgggtgtc aggtgcgaat
    20941 gattcgtgaa gacgagcgtt cagccgatca aagctcgccg gccggcttgg ccggggcatc
    21001 ttccgcgcct tcgaaggcgt aggcgccctt gagtgccgcc cagccttcct cggcggtctc
    21061 gacgaacttg aacaggtgca cgtcctctgg cgagatcatg cccgtctcga tcagcaggtc
    21121 gaagtcgatc agccgcttcc agaacgcggc gccgaacagc aggatcggcc gcgggcgcga
    21181 cttgccggtc tggatcagcg tgagcacctc gaacagctcg tccagcgtcc cgaagccgcc
    21241 cgggaagcac gccagcgcga cggcgcgcat caggaagtgc atcttgcgca gcgcgaagta
    21301 gtggaactgg aagcacagct cgggcgtgat gtacgggttg ggctgctgtt cgtggggcag
    21361 cacgatgttc aggccgatgc tcttgccgcc cgattcgaac gcgccgcggt tgccggcttc
    21421 catgatgccg ggcccgccgc cggtgacgac atggatcggc gtggcgaggt ggcgcgtcct
    21481 gacggtgacc agctccgcga agcggcgtgc ctcggcgtag tggttgctca tgttgaccgc
    21541 cgtctccgcg cgcctgatcg cctgcgcgtc gccgccgcgc atggcgtcgg cgagctgctg
    21601 ctgcgccgcc tcgggcgtct tgatgcgggc gctgccgaac agcacgatgg tcgactcgat
    21661 gccgtgcctg gcctgcacca tctccggctt catcagctcg agctgca
//
LOCUS       DATCRO010000146         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_69047, whole genome shotgun sequence.
ACCESSION   DATCRO010000146 DATCRO010000000
VERSION     DATCRO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69047"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..>2706)
                     /locus_tag="VIN75_04110"
     CDS             complement(287..>2706)
                     /locus_tag="VIN75_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HEY8973377.1"
                     /translation="GGHRFEATGIRVARDTTLSQAPDGRIWLSSGPRGTSALPAAPAA
                     QPSEGLREDPALVHSKRLLFDRRGYLWGTEAMRGGVYRIDAPARLADGRALRASAFDA
                     LHDRGGGLPSNVAVPLLEDREGTVWVGTNLGLASYHANNVRVPPGSKVGPGINTALAA
                     DAQGTLWLANGGVLQRLDADGAQVVADGLPDINAMLAADGTLWLMGGSWLGRLEHGRV
                     EHIEVPVSEPRWSLEALAGDGRGGLWASFMDHGVFHLRDGRWTALEGSIGRGRTAPTA
                     LLADAAGRLWAGYPDGRVLCVDGEQQRTYAGAEGPRVGAIATFARRGDELLVGGDRGV
                     ARWRDGRFEPLAADPAMLAGVSGIALTAQGEAWINGSRGVLRVSARDLARGFGGQAAA
                     YELFDYHDGLPGIALQATPVSTAAIDARGRVWFNTNQGAAWIDPAQVRRNPAAPATFV
                     QTLIAGDRRYAAAGEPRLPQRTSAVRVQYTATSLAMPDRVRFRYRLDGVDEDWQEAGT
                     RREASYANLGPGHYRFRVIAANEDGVWNEQGASLAFSIEPQFFQTGWFRALCLAAGAL
                     LAAGLYLWRLRLMAERIHLRLEERTRERERIARELHDTLLQGVQGLLLRLQALAAGLS
                     EGGRTREALDAAIRRARDVLMEGRDRIVALRGESEEGERLAHTLRAVGEEYRDGGSIA
                     FQVSTEGAERALCPAAADEVIDIAREAIRNAFRHAHAGCIDVRVAYLPQGLRIRVRDD
                     GIGIPLDILRHGGRPGHWGLVGMRERAGRLGATLAIRRGKARGTDVVLHVPGQVVFEA
                     GAHGRRA"
BASE COUNT          354 a         1034 c          961 g          357 t
ORIGIN      
        1 gacagggcat ccatgccctg atcaaaaagg cggggacatc gtgtccccgc ccccctgcgg
       61 gggccttttc gtccagccct caccgccggc gccaagggga cccggtagat caagagccgg
      121 agcagaagcg cacaagcgga acgccgcggc tgcgccgcga gctcttcgct tgattgtcgc
      181 cccgcccgtc atccccctct cccctccggg gagagggccg gggtgagggg gcgggcggag
      241 cgtagatgca ggaacggcga tcaagccaca cgccagaagc cgcacttcac gccctgcgcc
      301 cgtgcgcccc ggcttcgaac accacctgcc ccggcacatg cagcaccacg tccgtcccgc
      361 gagccttgcc gcgccggatc gccagcgtcg ctcccagccg cccggcgcgc tcgcgcatgc
      421 ctaccaggcc ccagtggccc ggcctgccgc cgtgccggag gatgtcgagc gggatgccga
      481 tgccgtcgtc gcgcacgcgg atgcgcaagc cctgcggcag gtatgccacg cgcacgtcga
      541 tgcagccggc atgcgcatgc cggaaggcgt tgcggatcgc ctcgcgggcg atgtcgatca
      601 cttcgtcggc ggccgcgggg cacaaggcgc gttccgcgcc ttcggtactt acctggaagg
      661 cgatgctgcc gccgtcgcgg tactcctcgc ccaccgcgcg cagcgtgtgc gccagccgct
      721 cgccttcctc gctttccccg cgcagcgcga cgatgcggtc gcgcccctcc atcagcacgt
      781 cgcgggcgcg gcggatcgcc gcgtccagcg cctcgcgcgt gcgcccgcct tcgctaagcc
      841 cggcggccag cgcctgcagg cgcagcagca agccttgcac gccctgcagc aacgtgtcgt
      901 gcagctcgcg cgcgatgcgt tcgcgctcgc gcgtgcgctc ttccaggcgc agatggatgc
      961 gctcggccat caggcgcagc cgccacaggt aaaggccggc ggccagcagc gcgccggcgg
     1021 ccaggcacag cgcgcggaac cagccggtct ggaagaactg cggttcgatc gagaaggcca
     1081 ggctggcgcc ctgctcgttc cacacgccgt cctcgttggc ggcgatcacg cggaagcggt
     1141 agtgcccggg acccaggttg gcatacgagg cttcccgccg cgtgcccgcc tcctgccagt
     1201 cttcgtccac gccgtcgagg cggtagcgga agcgcacgcg atcgggcatg gccaggctgg
     1261 tggcggtgta ctgcacgcgc accgcgctgg tccgctgcgg cagccgcggt tcgccggcgg
     1321 cggcgtaacg ccggtcgccg gcgatcaggg tctgcacgaa ggtggccggc gccgccgggt
     1381 tgcgccgcac ctgcgccggg tcgatccatg cggcgccctg gttggtgttg aaccacaccc
     1441 gtccgcgcgc atcgatcgcg gcggtcgata ccggcgtggc ctgcagggcg atgccgggca
     1501 ggccgtcgtg atagtcgaac agctcgtaag cggcggcctg gccgccgaag ccgcgcgcca
     1561 ggtcgcgcgc gctcacgcgc agcacgccgc ggctgccgtt gatccatgct tcgccctgcg
     1621 cggtaagcgc gatgccggac accccggcca gcatcgccgg gtccgccgcg agcggctcga
     1681 aacgtccatc gcgccagcgc gccacgccgc ggtcgccgcc gaccagcagc tcgtcgcccc
     1741 gccgggcgaa ggtggcgatg gcgccgacgc ggggcccctc ggcgccggca taggtgcgct
     1801 gttgctcgcc gtcgacgcac agcacgcggc catcgggata gccggcccac agccggcccg
     1861 cggcgtcggc gagcagcgcg gtgggcgcgg tgcggccgcg gccgatgctg ccttcgagcg
     1921 ccgtccagcg gccgtcgcgc agatggaaca cgccatggtc catgaagctc gcccacaggc
     1981 cgccgcggcc gtcgccggcc agcgcttcca ggctccagcg cggttcgctt accggcacct
     2041 cgatgtgctc cacgcggccg tgttccaggc ggcccagcca ggagccgccc atcagccaca
     2101 gcgtgccgtc cgcggccagc atcgcgttga tgtccggcag gccgtcggcg accacctgcg
     2161 cgccgtccgc gtcgagccgc tgcagcactc cgccgttggc cagccagagc gtgccctgcg
     2221 cgtcggcggc gagcgcggtg ttgatgccgg ggccgacctt gctgcccggc ggcacgcgca
     2281 cattgttggc gtggtagctg gccaggccca ggttggtgcc cacccacacg gtgccttcgc
     2341 ggtcttccag cagcggcacc gcgacattcg acggcaggcc gccgccgcgg tcgtgcaagg
     2401 cgtcgaacgc cgatgcgcgc aaggcgcggc catcggccag gcgcgccggc gcatcgatgc
     2461 ggtagacgcc gccgcgcatc gcctcggtgc cccacaggta gccgcggcgg tcgaacagca
     2521 gccgtttgga atgcaccagc gccgggtcct cgcgcaagcc ttccgatggc tgcgcggccg
     2581 gcgccgcggg cagggcgctg gtgccgcgcg gtccgctgga aagccagatc cgtccgtccg
     2641 gcgcctggct cagcgtggta tcgcgcgcca cgcggatgcc ggtcgcttcg aagcgatgcc
     2701 cgccac
//
LOCUS       DATCRO010000147         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_496763, whole genome shotgun sequence.
ACCESSION   DATCRO010000147 DATCRO010000000
VERSION     DATCRO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496763"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VIN75_04115"
     CDS             complement(<1..553)
                     /locus_tag="VIN75_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8973378.1"
                     /translation="MTDELDRTRSALADAQAALATLRAERDAAVDDARAAHRQRHAFL
                     AGLAHDLRNPLAPLSNGIELLRLTDNDPVGRLRTRGIMERQVGHLMRIADELSDLARL
                     SGTQATLQRGAFDIATVLHGVAERLAPTLQAQEMTLDIQADPGALPRVRGDAQRVGQA
                     VGYLVDDIVRHTERGSAIRVAATA"
     gene            566..787
                     /locus_tag="VIN75_04120"
     CDS             566..787
                     /locus_tag="VIN75_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973379.1"
                     /translation="MGLLTGLGNRGDIALALVPQLRDQRRGQPQARRQDDDEAREGHA
                     DQDEERDDGTLRQEPWASPDCTVPGPRFG"
     gene            complement(795..2252)
                     /gene="rpoN"
                     /locus_tag="VIN75_04125"
     CDS             complement(795..2252)
                     /gene="rpoN"
                     /locus_tag="VIN75_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8973380.1"
                     /translation="MGSMDFRTEQRQTQTLSPRLQHAVRLLQLSSLEFAQQVHDALGK
                     NPFLEEDSSDEAGDDALSPEQAAEAQEAREAQEAAAETMAAPEGEGPGEGDRDMWQAD
                     GLGSGKRADDDETSALDMMAVPTSLATHLHGQLAVLPLTLRDLVLAKVIVESLDDDGY
                     LRTELQEMIGVADLDPPATLEEMQIALKRVQALEPAGVAARSVSECLLAQLSTIEDED
                     AREMARHIIGEHLSCLAAKDVSTMARALGRTPAEIEAVCDRIRRLDPRPGWRFGSTQL
                     QYVIPDVIVRKVRGQWNVQLNPAIIPKVRLNQVYAELFQRHRSKQNPEMATHLQEAKW
                     TMRNVEQRFSTILSVAEAIVSRQKHFLEFGAMAMKPLGLREIADELGLHESTVSRVTN
                     NKYMATPIGVFELKYFFSRAMTTASGGACSGTAIRGLIKDIIEAEAPTAPLSDAEIAR
                     QLATQGLQVARRTVTKYRQLLKIEAVERRRRHTPS"
     gene            complement(2602..2793)
                     /locus_tag="VIN75_04130"
     CDS             complement(2602..2793)
                     /locus_tag="VIN75_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973381.1"
                     /translation="MKHVYPPRHPAWLDDKAAKAPPAAPDSPQPEAPREQGATTQAAP
                     GAPERQARSQPGEGGTRAS"
     gene            complement(2823..>3287)
                     /locus_tag="VIN75_04135"
     CDS             complement(2823..>3287)
                     /locus_tag="VIN75_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8973382.1"
                     /translation="VGKATYNTSCGSVAGLTCSKGTTSYSLTAGNMVTDNVGVELSAM
                     NLGKADRAGGSVIARGVNLSAVGRLPLGEHFALEGKVGPTWGVTKVSVPAGSALPSGR
                     ARGVGLGYGVALDVNFARGLHGAIGWEQHDFKFAGQGRSDVHNVTVSLGYMF"
BASE COUNT          431 a         1143 c         1188 g          525 t
ORIGIN      
        1 ccgccgtggc ggcgacgcgg atcgcgctgc cgcgctcggt gtggcgcacg atgtcgtcga
       61 ccaggtaccc gaccgcctgg ccgacgcgct gggcgtcgcc gcgcacgcgc ggcagcgcgc
      121 cggggtcggc ctggatgtcc agtgtcatct cctgcgcctg cagcgtcggc gcgaggcgct
      181 cggcgacgcc gtgcagcacg gtggcgatgt cgaacgcacc gcgctgcagc gtcgcctgcg
      241 tgccggacag ccgcgccagg tccgacagtt cgtcggcgat gcgcatcagg tggcccacct
      301 ggcgctccat gatgccgcgc gtgcgcagcc ggccgacggg gtcgttgtcg gtcaggcgca
      361 gcagctcgat gccgttggac agcggcgcaa gcggattgcg caggtcgtgc gcgaggccgg
      421 ccaggaaggc atggcgctgg cgatgcgcgg cccgggcgtc gtcgacggcg gcgtcgcgct
      481 cggcgcgcag cgtggcgagc gcggcctgcg cgtcggccag cgcgctgcgc gtgcgatcca
      541 gttcatcagt catcgaggac attcggtggg cctcctcacg ggcctgggca atcgtggcga
      601 catcgcgctc gcgctggtcc cgcagctccg cgatcaacgc cgaggccagc cgcaggcgcg
      661 ccgccaggac gacgacgaag cccgcgaagg ccacgcagat caggacgagg aacgcgatga
      721 cggaactctt cggcaggaac cttgggcaag ccccgactgt acggtgcccg gcccgcggtt
      781 cggatgacgg ccgctcagga cggcgtgtgg cggcgccggc gctcgacggc ctcgatcttg
      841 agcagctggc ggtacttggt gacggtgcgg cgcgcgacct gcaggccctg cgtcgcgagc
      901 tggcgcgcga tctcggcgtc ggacagcggc gcggtgggcg cctcggcctc gatgatgtcc
      961 ttgatcaggc cgcggatggc cgtgcccgag catgcgccgc cgctggcggt ggtcatggcc
     1021 cgcgagaaga agtacttcag ctcgaacacg ccgatcggcg tggccatgta cttgttgttc
     1081 gtcacgcgcg acacggtgga ctcgtgcagc cccagttcgt cggcgatctc gcgcaggccc
     1141 agcggcttca tcgccatcgc gccgaattcg aggaagtgct tctggcgcga gacgatggcc
     1201 tcggccacgc tcaggatggt ggagaagcgc tgttcgacgt tgcgcatcgt ccacttggct
     1261 tcctgcaggt gcgtggccat ctccgggttc tgcttgctgc gatggcgctg gaacagctcc
     1321 gcgtagacct ggttcaggcg caccttggga atgatggcag gattgagctg cacgttccat
     1381 tggccgcgca ccttgcgcac gatcacgtcc gggatgacgt actgcagctg cgtcgagccg
     1441 aagcgccagc ccgggcgcgg gtcgagccgg cggatgcggt cgcagacggc ctcgatctcg
     1501 gccggcgtgc ggcccagcgc gcgcgccatc gtgctcacgt ccttggcggc caggcagctc
     1561 aggtgttcgc cgatgatgtg gcgcgccatc tcgcgcgcgt cctcgtcctc gatggtggac
     1621 agctgcgcca gcaggcattc ggagacgctg cgcgcggcca cgccggccgg ctccagcgcc
     1681 tgcacgcgct tgagcgcgat ctgcatctcc tcgagcgtgg ccggcgggtc gaggtcggcc
     1741 acgccgatca tttcctgcaa ctcggtgcgc aggtagccgt cgtcgtccag cgattcgacg
     1801 atcaccttgg ccagcaccag gtcgcgcagc gtcagcggca gcaccgccag ctggccgtgc
     1861 aggtgcgtgg ccagcgacgt gggcacggcc atcatgtcga gggcgctggt ctcgtcgtcg
     1921 tcggcgcgct tgcccgagcc gaggccgtcg gcctgccaca tgtcgcggtc gccctcgccc
     1981 gggccctcgc cttccggcgc ggccatcgtc tcggcggcgg cctcctgcgc ctcgcgcgct
     2041 tcctgcgcct cggcggcctg ctcgggcgag agcgcgtcgt cgccggcctc gtccgacgaa
     2101 tcctcttcca ggaacggatt cttgcccagg gcgtcgtgga cttgctgagc gaattcgagc
     2161 gatgaaagct gcagcagccg gacggcgtgc tgcaggcggg gggacagcgt ctgtgtctgt
     2221 cgctgttccg tgcggaagtc catcgaaccc attggttcct cctgagcgtg ggacccgcga
     2281 gcccggcaag agttgccgga ttcgcgttca tgccggggag gtacgcaacg tgcgtgccag
     2341 aaatcacgag cccgggtaat cccgggcctg aacgcttgca aatgaccgga aacggcgcga
     2401 ttgccccggt tcgcgcgcac ttttgcggtc aacagcggct cgattgcttc acggatcgga
     2461 acgttcaacc gcattcaagc tgaacgacag cgcgtccggg ccccagattt gccgcaagcg
     2521 cggactgcgc agcggcgtgg tggctggcag gcgtcctggc gctcatccac tggcctgcat
     2581 gccgcgcccg ggcggcggcc gtcaagaggc gcgagtgccg ccctcgccgg gctgactccg
     2641 cgcctgccgt tccggcgctc cgggagccgc ctgcgtcgtc gcgccctgct cgcgtggcgc
     2701 ctcgggctgc ggcgagtccg gcgccgcggg cggcgccttc gcggccttgt cgtcgagcca
     2761 tgcgggatgc cggggcggat agacgtgctt catcgcgatg gactcctcgt gcaggcgccg
     2821 gctcagaaca tgtagccgag cgacaccgtc acattgtgga cgtccgagcg gccctggccg
     2881 gcgaacttga agtcgtgctg ttcccagccg atcgcaccat gcaggccgcg cgcgaagttc
     2941 acgtcgagcg cgacgccgta gcccaggccc acgccgcgcg cgcggccgct cggcagggcc
     3001 gagcccgccg gcacgctgac cttcgtgacg ccccaggtcg ggcccacctt gccttcgagc
     3061 gcgaagtgct cgccgagcgg caggcggccc acggcgctca ggttgacgcc gcgcgcgatc
     3121 acgctgccgc ccgcgcggtc ggccttgccc aggttcatcg cgctcagctc gacgcccacg
     3181 ttgtcggtga ccatgttgcc ggcggtcagg ctgtacgagg tggtgccctt ggagcacgtc
     3241 aggcccgcga cgctgccgca ggacgtgttg tacgtggcct tgccgac
//
LOCUS       DATCRO010000148         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_699202, whole genome shotgun sequence.
ACCESSION   DATCRO010000148 DATCRO010000000
VERSION     DATCRO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699202"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..831)
                     /gene="maiA"
                     /locus_tag="VIN75_04140"
     CDS             complement(145..831)
                     /gene="maiA"
                     /locus_tag="VIN75_04140"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY8973383.1"
                     /translation="MTTPADDDRFELYGFWRTSATFRVRVAFHLKGVKPRETVIDMDG
                     GEQFGEAYLRINPLGGLPALVDHGPGQPPVPLTQSLAILEFIDELYPQPPMLPADAHG
                     RARVRSIAQMLAADTHPLITPRVRKYLATVGGFDADASRAWLVHWLSTGLQAVERRLA
                     TEPGTGRFCHGDQPTIADICLASLFMVTRVLKVDVADIPVSTRIVAECERLPAFAAAA
                     PALQVGAPAL"
     gene            complement(878..1783)
                     /locus_tag="VIN75_04145"
     CDS             complement(878..1783)
                     /locus_tag="VIN75_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973384.1"
                     /translation="MRITLRQLAVFTAVADAGSTTAAAGRVALSQSATSAALNELESV
                     LQARLFDRVGTRLVLNDTGRGLLPQARAVLDGAAGIERDFGIAVGARDADAPSLLRVG
                     ASTTIGNYLLPTLIAQWRRARPGAQVDVAIANTAAVAAAVARMEVDIGLIEGPCHEPD
                     VVARPWREEELVICAAPAHPLLAGDPARKLDVAALRRADWLLREPGSGTREAVEQALQ
                     PHLHQLREGLRLGGTEAIKQAAVEGLGLTCLSLFAVRDLVALGRLVRVRTTLPRLARR
                     FWLVHHRRKQFSRGLAAFVDFCEQG"
     gene            1891..2961
                     /locus_tag="VIN75_04150"
     CDS             1891..2961
                     /locus_tag="VIN75_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEY8973385.1"
                     /translation="MTAASRTFVAPPVPVPAMRLLPGLLLAAAVASAAAALGHIGWLQ
                     AHGLSALTLAIALGLVAGNLLPAPALAASAEGLNLAKQRLLRAGIVLYGLRLTFQDIG
                     HVGLPGIAIDAAVLASTFTLACTLGPRLFGLDRTTSILIGAGSSICGAAAVLATEPVV
                     RGRPEQVAVAVATVVGFGTLAIFLYPALYHLVAAAHPLSPTAYGLWAGSTIHEVAQVV
                     AAGHAAGDAAADTAVIAKMVRVMMLAPFLVALSAWVARSRPASSGLRRAPVTIPWFAF
                     GFVALAGLRSLDVLPQPALAIAGDIDLALLAVAMAALGLTTRLAAMKAAGLAPLKLGA
                     ALAAWLVIGGAAINVVVAALAR"
     gene            complement(2967..>3512)
                     /locus_tag="VIN75_04155"
     CDS             complement(2967..>3512)
                     /locus_tag="VIN75_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY8973386.1"
                     /translation="GLPSGYVPFNVQNLGGSLFVTYARRDGGSTDETAGAGFGFVDRY
                     DLQGNLLGRVASGGVLDAPWGLAIAPPSFGALAGSLLVGNFGDGHISAYNLATDSFMG
                     QLTTGNGQALAIDGLWGLSVGNDGGAGSSDMLYFTAGPGDESHGLFGVMQAVPETNTV
                     AMLLAGLAMFAWRIGRRATLR"
BASE COUNT          436 a         1316 c         1288 g          472 t
ORIGIN      
        1 gggcctgcgc ctcgaacggg tgcgagtggg tctccaccac ctggcccggg ttccagcggc
       61 gctcccgcac gtcgtcggcg ccgtgggcgc gcgcggcggc ctcgaactgt tcgaaggtgg
      121 gcgatggcat ggcgcgggct ccgttcagag cgccggcgcg ccgacctgca gcgccggcgc
      181 ggcggccgcg aaggccggca ggcgttcgca ctcggccacg atgcgcgtgg acaccgggat
      241 gtcggcgacg tcgaccttga gcacgcgcgt gaccatgaac aggctggcca ggcagatgtc
      301 ggcgatcgtc ggctggtcgc cgtggcagaa gcgaccggtg cctggctcgg tggccaggcg
      361 ccgttcgacc gcctgcaggc ccgtggacag ccagtgcacg agccacgcgc gcgacgcgtc
      421 cgcgtcgaag ccgccgaccg tggccaggta cttgcgcacg cgcggcgtga tcagcggatg
      481 ggtgtcggcc gccagcatct gcgcgatgga gcgcacgcgg gcccggccgt gcgcgtcggc
      541 gggcagcatc ggcggctgcg ggtagagctc gtcgatgaac tcgaggatgg ccagcgactg
      601 cgtcagcggt accggcggct ggccggggcc gtggtcgacg agcgccggca acccgcccag
      661 cggattgatg cgcaggtagg cctcgccgaa ctgctcgccg ccgtccatgt cgatgaccgt
      721 ctcgcgcggc ttgacgccct tgaggtggaa cgccacgcgc acccggaagg tggcggaagt
      781 gcgccagaag ccgtacagct cgaagcggtc gtcgtcggcg ggcgtggtca tgcgcggtcc
      841 tccagcgtgg gtcgatcgcg acgataccgc agcgcgctca accctgctcg cagaagtcga
      901 cgaaggccgc cagcccgcgc gagaactgct tgcgccgatg atggacgagc cagaagcgcc
      961 gcgccaggcg gggcagcgtc gtgcgcacgc gcacgaggcg ccccagcgcg accaggtcgc
     1021 gtacggcgaa cagcgacagg caagtcaggc ccaggccctc gaccgccgcc tgcttgatgg
     1081 cctcggtgcc gcccaggcgc agcccctcgc gcaactggtg caggtgcggc tgcagggcct
     1141 gctccacggc ctcgcgcgta cccgagcccg gctcgcgcag cagccagtcg gcccggcgca
     1201 gcgccgcgac gtcgagcttg cgcgcggggt cgccggccag cagcggatgc gcgggcgccg
     1261 cgcagatcac gagctcttcc tcgcgccacg gccgcgcgac gacatcgggc tcgtggcagg
     1321 gcccttcgat caggccgatg tcgacctcca tgcgcgccac cgcggcggcc acggccgcgg
     1381 tgttggcgat ggccacgtcg acctgcgcgc cgggccgcgc gcggcgccac tgcgcgatca
     1441 gcgtgggcag caggtagttg ccgatggtcg tgctggcgcc cacgcgcagc agcgagggtg
     1501 cgtccgcgtc ccgggcgccc acggcaatgc cgaagtcgcg ctcgatgccc gccgcgccat
     1561 ccagcaccgc gcgcgcctgc ggcagcaggc cccggccggt gtcgttgagg accaggcgcg
     1621 tgccgacgcg gtcgaacagc cgggcctgca gcaccgactc gagctcgttg agcgccgcgc
     1681 tggtggccga ctgcgacagc gccacgcggc ccgcggcggc cgtcgtgctg ccggcgtccg
     1741 ccacggcggt gaagacggcc agctggcgca aggtgatccg catgcccgca gtctacctat
     1801 gaagcaggta gcctcaacga aatctaatcg ttttgcagat agacggcggg cgcgcacagt
     1861 gtggccatcg ccccgcatcc agtcaccgtc atgaccgccg cctcccgcac cttcgtcgcc
     1921 ccgcccgtcc ccgtccccgc gatgcgcctg ctgcccggcc tgctgctggc cgcggccgtc
     1981 gccagcgccg ccgcggccct gggccacatc ggctggctgc aggcgcacgg gctgtccgcg
     2041 ctgacactgg ccatcgcgct gggcctggtc gcgggcaacc tgctgccggc gcccgcgctg
     2101 gcggcctccg ccgagggcct gaacctggcc aagcagcgcc tgctgcgcgc cggcatcgtg
     2161 ctgtacggcc tgcggctgac cttccaggac atcggccacg tcggcctgcc cggcatcgcg
     2221 atcgacgcgg ccgtgctcgc cagcacgttc acgctcgcct gcacgctcgg cccgcgcctg
     2281 ttcgggctcg atcgcacgac gtccatcctg atcggggcgg gcagctcgat ctgcggcgcg
     2341 gcggccgtgc tggccaccga gccggtggtg cgcggccggc ccgagcaggt cgccgtcgcc
     2401 gtggccacgg tcgtgggctt cggcaccctc gccatcttcc tgtacccggc gctgtaccac
     2461 ctggtcgcgg cggcgcaccc gctgtcgccg accgcgtacg gcctgtgggc cggctcgacg
     2521 atccacgagg tcgcgcaggt cgttgcggcc ggccatgccg cgggcgacgc ggcggccgac
     2581 accgccgtca tcgcgaagat ggtgcgcgtg atgatgctgg cgcccttcct cgtggcgttg
     2641 tcggcctggg tcgcgcgctc gcgccccgca tcgtccggcc tgcggcgcgc gcctgtcacg
     2701 attccctggt tcgccttcgg cttcgtcgcg ctggccggac tgcgctcgct cgacgtgctg
     2761 ccgcagcccg ccctcgcgat cgccggcgac atcgacctcg cgctgctcgc ggtggcgatg
     2821 gccgcgctgg gcctgacgac gcgcctggcc gcgatgaagg cggccggcct cgccccgctg
     2881 aagctgggcg cggcgctggc cgcctggctg gtgatcggcg gcgcggccat caatgtcgtc
     2941 gtggccgcgc tcgcccgctg aggccgctag cgcagggtcg cgcgccgccc gatgcgccat
     3001 gcgaacatcg cgaggccggc cagcagcatc gccaccgtgt tggtctccgg caccgcctgc
     3061 atcacgccga acaggccgtg cgactcgtcg cccgggccgg ccgtgaagta cagcatgtcg
     3121 ctgctgccgg cgccgccgtc gttgcccacg ctcaggcccc acaggccgtc gatggccagc
     3181 gcctggccgt tgccggtggt cagctggccc atgaacgagt cggtggccag gttgtacgcg
     3241 ctgatgtggc cgtcgccgaa gttgcccacc agcagcgagc ccgccagcgc gccgaacgac
     3301 ggcggcgcga tcgccaggcc ccatggcgcg tcgagcacgc cgccgctggc cacgcggccc
     3361 agcaggttgc cctgcaggtc gtagcggtcg acgaagccga agccggcgcc ggcggtctcg
     3421 tcggtgctgc cgccgtcgcg ccgggcgtag gtgacgaaca gcgaaccacc gaggttctgc
     3481 acgttgaacg gcacgtagcc cgagggcagg cc
//
LOCUS       DATCRO010000149         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_102873, whole genome shotgun sequence.
ACCESSION   DATCRO010000149 DATCRO010000000
VERSION     DATCRO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102873"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..548)
                     /locus_tag="VIN75_04160"
     CDS             complement(174..548)
                     /locus_tag="VIN75_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973387.1"
                     /translation="MTPPIVKPVADPLDDIDRIAPVDNLMARQDRADLPGNRSDIPRN
                     DDAPRDEADDALDDSSEDAGALPDPDDVALPVDRGNDVSLDDPDVAATLENAQFERTG
                     RGPDDDEPIEETERLGDDSRTG"
     gene            722..1276
                     /locus_tag="VIN75_04165"
     CDS             722..1276
                     /locus_tag="VIN75_04165"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEY8973388.1"
                     /translation="MKLPLPPVLFAFLALALLLVLAVAAVRRSKGAARGGPVALKGRP
                     LMNEREQAMYQRLKETFPGSVVLAQVAFSALVASTREHRHRFDRLVADFVLCDASMKV
                     QVVIELADPGSKSRARLDAVRSKVLAKAGYKVLRFANMPGQDDLRAYLNAQMPADDRA
                     ASPRRTSSRSAVVKATRSMALDEI"
     gene            1428..2357
                     /locus_tag="VIN75_04170"
     CDS             1428..2357
                     /locus_tag="VIN75_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973389.1"
                     /translation="MNSRLHPTRVAAATALLALAAAPWAARADTPPPNDGMRFVSAGA
                     QSDNHGNREVLSTLSLPVGQHAWVQAGGGASRSNAAGGGRRPGVATAGVGVAGQQLQL
                     TVNTAQRFDGRRYRQSDVGSSLDWKQDGNDIGVDVTHRRSSAAGMASVSNGVGGSTSV
                     PAQARVAGNGVGVHGALQVTERVTVYGAVARNHYKTRTEQGDPSSGGLLGSNPILARA
                     LLGGTSVVNRDEVDLDHSAQVGATYRWDKVAVSAEATTGAVHDGGGTLRSVDLKAAID
                     VAPGWRVSPGVGHGTSEQGGHATYASLAATYGW"
     gene            2603..>3101
                     /locus_tag="VIN75_04175"
     CDS             2603..>3101
                     /locus_tag="VIN75_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEY8973390.1"
                     /translation="MNGDPSSPWLLLVLSLPTPSATARMRVWRALKALGCMALRDGAY
                     LLPQGEGRDAALMEIADDCTREGGVAWLVQARPRGADDEASWRALFDRSGDYAELRKS
                     WKEASRGLGKLGAAELARLQRRVQREFGSIRAIDFFPGDASTEAEAACAEFGKRIEAL
                     LSPDEP"
BASE COUNT          425 a         1095 c         1149 g          432 t
ORIGIN      
        1 accacgacgg gcagaccccg tggccgtcgc tggccgcgct cagcaccttg ctggacggcc
       61 ccgcggtggg ccacttcgcc agccggcgcg gcacgcagga gtggatcgtc gagcgcggcg
      121 ccgcgcgcat cgccggcgac cgcgatgccg acgggcccgg cgcccgccag tggtcagccg
      181 gttcgcgaat cgtcgcccag acgctcggtc tcctcgatcg gctcgtcgtc atcgggtccg
      241 cggcccgtgc gctcgaactg cgcgttctcg agcgtggcgg ccacgtcggg gtcgtccagc
      301 gagacgtcgt tgccgcggtc gaccggcagc gcgacgtcgt ccgggtcggg cagggcgccg
      361 gcgtcctcgg acgagtcgtc gagcgcgtcg tcggcctcgt cgcgcggcgc gtcgtcgttg
      421 cgcgggatgt cggagcggtt gcccggcagg tcggcgcgat cctggcgcgc catcaggttg
      481 tcgacggggg cgatgcggtc gatgtcgtcg agcggatcgg ccacgggctt gacgatgggt
      541 ggggtcatcg ggatctccgg ttgggttgcg caggctgcgg caaccggagt gccggcgcac
      601 gcgaggcgcg tcggcaaggt gaacaaggtc acgctgtccg cgcttatgtc ggccgggggc
      661 cgggaaacgg tcgaggtggc gtcagaatcg cgcgcgtgtc cgtccaccga aatccgcttc
      721 gatgaagctg ccgctcccgc ccgtcctgtt cgcgttcctg gcgctcgcgc tgctgctggt
      781 gctggccgtc gcggccgtgc ggcgctccaa gggcgcggcg cgcggcggcc cggtcgccct
      841 gaagggccgc ccgttgatga acgagcgcga gcaggcgatg taccagcgtc tcaaggagac
      901 cttcccaggc tccgtggtgc tggcgcaggt cgcgttctcg gcgctggtgg ccagcacgcg
      961 cgagcaccgg catcgcttcg accgcctggt ggccgacttc gtgctgtgcg acgcgtcgat
     1021 gaaggtgcag gtcgtcatcg agctcgccga ccccgggagc aagagccgcg cgcgcctgga
     1081 cgccgtgcgc tcgaaggtgc tcgccaaggc cggctacaag gtgcttcgct tcgcgaacat
     1141 gcccggccag gatgacctgc gcgcctacct gaacgcccag atgcccgccg acgaccgcgc
     1201 cgcctcgccg cgcaggacgt cctcgcgcag cgccgtcgtc aaggcgacgc ggagcatggc
     1261 gctggacgag atctgacgag gcccgcgcca ggatagatct gatgccggac gcttggcagg
     1321 cgtccgggta cgccggggcg gcgaagaatt tatcggaggc tgggaagaaa cggccggcac
     1381 ccatcgttgt gcagtacatg aactgccaac ccgctggaca ccgccccatg aactcccgcc
     1441 tccacccgac ccgcgtggcc gctgccaccg ccttgctcgc gctcgccgcc gctccctggg
     1501 ccgcccgggc cgacaccccg ccgcccaacg acggcatgcg cttcgtcagc gccggcgcgc
     1561 agtcggacaa ccacggcaac cgggaggtgc tgtcgacgct gagcctgccc gtgggccagc
     1621 atgcgtgggt gcaggcgggc ggcggcgcct cgcgcagcaa tgcggccggt ggcgggcgcc
     1681 ggccgggcgt cgccaccgcg ggcgtcggcg tcgccggcca gcagctgcag ctgaccgtca
     1741 acacggccca gcgcttcgat ggccgccgct atcgccagtc ggacgtgggc tcgtcgctcg
     1801 attggaagca ggacggcaac gacatcggcg tcgacgtcac gcatcgccgc tcgagcgccg
     1861 cgggcatggc ctccgtgtcg aacggcgtcg gcggatcgac cagcgtcccg gcgcaggccc
     1921 gcgtcgcggg caacggcgtc ggcgtgcacg gggcgctgca ggtcaccgag cgcgtcaccg
     1981 tctatggcgc ggtcgcgcgc aaccactaca agacccgcac ggaacagggc gacccgtcat
     2041 cgggcggcct gctcggttcg aacccgatcc tggcgcgtgc gctgctcggt ggcacatcgg
     2101 tcgtcaaccg cgacgaggtc gacctcgacc acagcgcgca ggtcggcgcg acctaccgct
     2161 gggacaaggt ggccgtgtcg gccgaggcca ccaccggcgc ggtgcacgac ggcggcggca
     2221 ccctgcgcag cgtcgacttg aaggccgcga tcgatgtcgc gccgggctgg cgcgtatcgc
     2281 cgggcgtggg ccacggcacg agcgagcagg gcggccacgc gacgtacgcg tcgctggcgg
     2341 cgacgtacgg ttggtgatgg ccgtcaacga agcgtcatgc cggcatgacg cgtcgagcgc
     2401 ttcgcgtgac ggcgcacgcg tgcatggcgc gtcgagtagt tcgcgtcgcg cactgtcgcc
     2461 gcatcgcgcc tacccggcgt catcctgcgc gcagtcgcag gatccacgcg cgctcgctgt
     2521 ttcgtatcgc ggagccgaaa gggcgccgtc acgagaagga catcgcttgt agagtaaacg
     2581 acacagaagt tatagtcgcg acatgaacgg tgatccatcg tcgccctggc tgctgctggt
     2641 gctctcgctc ccgaccccca gcgcgaccgc gcgcatgcgg gtctggcgcg cgctgaaggc
     2701 actcggctgc atggccctgc gcgacggggc gtacctgctg ccccagggcg agggccgcga
     2761 cgcggcgctg atggagatcg ccgacgactg cacgcgcgaa ggcggcgtgg cgtggctggt
     2821 gcaggcccgg ccgcggggcg ccgacgacga ggcgtcctgg cgcgccctgt tcgaccgcag
     2881 cggcgactat gcggaactgc gcaagtcctg gaaagaggcc agccgtggcc tgggtaagct
     2941 cggcgcggcc gaactcgcgc gcctgcagcg ccgcgtccag cgcgaattcg ggtcgatccg
     3001 cgccatcgac ttcttcccgg gcgacgcgag caccgaggcc gaggccgcgt gcgcggagtt
     3061 cggcaagcgc atcgaggcgc tgctctcgcc cgacgagcca c
//
LOCUS       DATCRO010000150         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_586733, whole genome shotgun sequence.
ACCESSION   DATCRO010000150 DATCRO010000000
VERSION     DATCRO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586733"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1442
                     /locus_tag="VIN75_04180"
     CDS             204..1442
                     /locus_tag="VIN75_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEY8973391.1"
                     /translation="MPSRFLLTPIAALCLAAALPVAGAEPGPAVVVAQLGPTSPGFRM
                     AVKRGDTGAPEATAVGATPIDRRAPAASSAEWRSAASHPSTNKDEPEHKDQKSAKADA
                     AKTAASGSPMDQLREKLAARLGATPSPNGRFALQVSNRGESGENVVTSVRPGAAAVAH
                     ARRRHAAASAAAGDANMDIAANAGQSLLPTHWDYEGSAGPEAWGRLRPEYSKCASGQR
                     QSPIDIRGGIAVDLEPIKFDYRPSAFSVIDNGHTVQVDVEPGNAITITGRRYELVQFH
                     FHRPSEERVNGRQYDMVVHLVHKDSEGRLAVVAVLLDRGSAQAVVQAVWNNLPLEKGD
                     EVRAGTRIDLAQLLPEDKRYYTYMGSLTTPPCSEGVLWMVMKQPVPISPEQVAIFARL
                     YPMNARPIQQADGRLIKESN"
     gene            1574..2176
                     /locus_tag="VIN75_04185"
     CDS             1574..2176
                     /locus_tag="VIN75_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8973392.1"
                     /translation="MKPIRLLIIAGSERAGAYSRRLARVAAAAARRDGCEVTEFDLRA
                     LALPIYDGDLEHASGVPAAALQLRDALKSNDAMLLVTPEYNGFPTPLVLNAFDWMSRI
                     PATADGGAGLVATANKPVALLSSSPGALGGLRAMTLMRQYLQGNFAMIVAPQQFALSR
                     AGEAFDDGGTLKDAKAQQSVEHVLAAIGELATALKGGASR"
     gene            complement(2342..3628)
                     /locus_tag="VIN75_04190"
     CDS             complement(2342..3628)
                     /locus_tag="VIN75_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY8973393.1"
                     /translation="MIRRLLAALALAAIALPAAAHKASDAYVTLRVEGADVSARVDVA
                     LRDLDRDLDLDADADDRLTWREVRTRWSDIVELTRAGLVPIADGAACTPANDGEAAPA
                     LATHSDGRYAVVRLSWHCASAVQRLDAGYRLFARTDPTHRGIVRYARGGDPLQLAVLS
                     PGAGAVRLRLPALPPAATAAVPDAGATVPAATPPVPAAPPSRVPVGATDTDANSGLAA
                     FGGFVREGIHHILIGYDHILFLLSLLLPAVWQRRREADAATGRTRTRWVPATDGRRAL
                     VGVLKVVTAFTLAHSITLALSVFDVLDPPSRWVESTIAASVVLAALNNVWPLIAEARW
                     KLTFVFGLVHGFGFASALKDAGLAHGDLVSPLLGFNLGVEIGQLCIVGLVLPLAWSLR
                     ATRTYRGAFAGGSLTIAAIAALWLVQRAFDLSLIAG"
     gene            complement(3625..>4317)
                     /locus_tag="VIN75_04195"
     CDS             complement(3625..>4317)
                     /locus_tag="VIN75_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973394.1"
                     /translation="LQGRDAAATATLARLAASTSADEPGDAPGWLQLMRAELAQRRGD
                     PAAGPLFAAALRSNSDVYTLCAYADWLLDAGRPGEVLTLLTGHDAADPVLLRVALAER
                     AAYGARDARTRRDTRLLGERFGAALLRGDRSHGREQSRYELDLRGDPAAALRLAQANW
                     RVQHEPADALALVRAAHAAGRDAAAADVWRFLRETGGRDARLAVGGPAAVPFLPAPIA
                     ARDSPSMNGSAP"
BASE COUNT          620 a         1550 c         1617 g          530 t
ORIGIN      
        1 tgatctcgcc gctcgtgatg cattccacgg tgctgctggc gctggcctcg ctgctgatgt
       61 cgtgcatcgg catcgtgtcg tggatcttcg tgcgcaaggc cgtcattccg gccgagccgg
      121 cgcattgatg caaaggtaac ggcgctcaag tcgcggccgg tccggccgat ctgtgggaag
      181 cacgccgtcc gtgcggagtc tccatgccgt cccgattcct gctcaccccc atcgccgccc
      241 tgtgcctggc cgccgcactg cccgtcgctg gcgcggagcc gggtccggcc gtcgtcgtgg
      301 cccagctggg gccgacctcg ccgggcttcc ggatggccgt caagcgcggc gacaccggcg
      361 caccggaggc cacggcggtc ggcgcgacgc ccatcgaccg ccgcgccccc gcggccagca
      421 gcgccgagtg gcgcagcgcc gcgtcgcacc cctcgacgaa caaggacgag cccgagcaca
      481 aggaccagaa gtccgccaag gccgacgccg cgaagaccgc cgccagcggc agcccgatgg
      541 accagctgcg cgagaagctc gctgcccgcc tgggtgccac gccgtcgccc aacggccgct
      601 tcgcgctgca ggtgtccaac cgcggcgaat cgggcgagaa cgtggtcacg tcggtgcgcc
      661 ccggcgcggc ggccgtggcc cacgcgcgcc gcaggcacgc cgccgcctcg gcggcggccg
      721 gcgacgccaa catggacatc gccgccaacg ccggccagtc gctgctgccc acccactggg
      781 actacgaagg cagcgccggc cccgaggcct ggggccggct gcgccccgag tacagcaagt
      841 gcgcgagcgg ccagcgccag agcccgatcg acatccgcgg cggcatcgcc gtcgacctgg
      901 agccgatcaa gttcgactac cggcccagcg cgttctcggt gatcgacaac ggccacaccg
      961 tgcaggtcga cgtcgagccg ggcaacgcca tcacgatcac cggacggcgc tacgagctgg
     1021 tgcagttcca cttccatcgc ccgtccgagg aacgcgtcaa cggccgccag tacgacatgg
     1081 tggtgcacct ggtgcacaag gattccgagg gccgcctggc ggtcgtggcg gtgctgctcg
     1141 atcgcggcag cgcgcaggcc gtcgtgcagg ccgtgtggaa caacctgccg ctcgagaagg
     1201 gcgacgaggt gcgcgccggc acgcgcatcg acctcgcgca gctgctgccc gaggacaagc
     1261 gctactacac ctacatgggc tctctcacca cgccgccgtg cagcgaaggc gtgctgtgga
     1321 tggtgatgaa gcagccggtg ccgatctcgc cggaacaggt ggcgatcttc gcgcgcctgt
     1381 acccgatgaa cgcgcggccg atccagcagg ccgacgggcg gttgatcaag gaatcgaact
     1441 gatccggtcg cggacttcgt gaggcggccc ggacggccgg ggtggatcct gcgcggagcg
     1501 aagcgcagtc gcggggacca cgcgccgcga gggtatcctc gtcgcatctt ccgacgcggt
     1561 cccgagcgcc ttcatgaaac ccatccgcct cctgatcatc gccggcagcg aacgcgccgg
     1621 cgcctacagc cgccgcctgg cgcgcgtggc cgcggccgcc gcgcgccgcg atggctgcga
     1681 agtcaccgaa ttcgacctgc gcgcgctcgc gctgccgatc tacgacggcg acctcgagca
     1741 tgcgtcgggc gtgccggccg ccgcgctgca actgcgcgac gcgttgaagt cgaacgacgc
     1801 gatgctgctg gtcacgcccg agtacaacgg ctttccgacg ccgctggtgc tcaacgcgtt
     1861 cgactggatg tcgcgcatcc ccgccaccgc cgacggcggc gcggggctcg tggccaccgc
     1921 caacaagccg gtggcgctgc tctcgtcgtc gcccggcgcg ctgggcggcc tgcgcgcgat
     1981 gacgctgatg cggcagtacc ttcagggcaa cttcgcgatg atcgtcgcgc cgcagcagtt
     2041 cgcgctgtcg cgcgcgggcg aggcgttcga cgacggcggg acgctgaagg atgcgaaggc
     2101 gcagcagtcg gtggagcatg tgctggcggc gatcggggag ctggcgacag cgttgaaggg
     2161 tggggcgagc cggtgagcct gcgggcgtcc tgaggcgcct gccgaggtgg atctgcgact
     2221 gcgcgcagga tgacgcctgg tcgcgcagga tgaggcctgg tttgcgcgat gcgcgagggt
     2281 ggcgtcacgc ggagcgccaa ccctgtcatc ctgaacgcta gtgtgctgtc ccggaagtac
     2341 actagcccgc gatcagcgag aggtcgaacg cgcgctgcac cagccacagc gcggcgatgg
     2401 cggcgatcgt caacgagccg ccggcgaacg cgccgcggta ggtgcgtgtg gcgcgcaggg
     2461 accaggccag gggcagcacc agccccacga tgcacagctg gccgatctcc acgccgaggt
     2521 tgaatcccag cagcggcgag acgaggtcgc cgtgcgccaa tcccgcatcc ttcagcgccg
     2581 aggcgaagcc gaagccgtgc accaggccga agacgaaggt gagcttccag cgcgcctcgg
     2641 cgatcagcgg ccacacgttg ttgagcgctg ccagcacgac gctggcagcg atcgtggact
     2701 cgacccagcg cgaaggcgga tccagcacgt cgaacaccga cagcgccagc gtgatcgagt
     2761 gcgccagcgt gaaggcggtg acgaccttga gaacgcccac cagcgcgcgc cggccatcgg
     2821 tcgccggcac ccagcgcgtg cgcgtccggc cggtcgcggc atccgcctcg cgccggcgct
     2881 gccagaccgc gggcagcagc agcgacagca ggaacaggat gtggtcgtag ccgatcagga
     2941 tgtgatggat gccttcgcgc acgaagccgc cgaatgccgc gagccccgag ttcgcgtcgg
     3001 tgtcggtcgc ccccaccggc acgcggctcg gtggcgcggc gggcaccggc ggcgtcgcgg
     3061 cggggacggt ggcaccggcg tcgggcacgg ctgccgtggc cgcgggcggc aacgcgggca
     3121 ggcgcagccg caccgcgccc gcgccggggc tcagtaccgc gagctgcagc ggatcgccgc
     3181 cgcgcgcgta gcggacgatg ccgcggtggg tcgggtcggt gcgcgcgaac aggcggtagc
     3241 cggcgtcgag ccgctgcacc gcagaagcgc agtgccagga caggcgcacg acggcgtagc
     3301 ggccgtcgct gtgcgtggcc agtgcgggcg cagcctcgcc gtcgttcgcg ggggtgcagg
     3361 ccgcgccgtc ggcgatcggc acgaggccgg cgcgcgtgag ctcgacgatg tcgctccagc
     3421 gcgtgcgcac ctcgcgccag gtcaggcggt cgtccgcatc ggcgtcgagg tccagatcgc
     3481 ggtcgaggtc ccgcagcgcg acgtccacgc gcgcggagac gtcggcgccc tccacgcgca
     3541 gcgtcacgta ggcgtcgctg gccttgtgcg ccgcagcggg gagggcgatc gcggcgagcg
     3601 cgagcgcggc gagcaggcgt cggatcatgg agcgctcccg ttcatcgagg gactgtcccg
     3661 cgccgcgatc ggcgcaggca ggaaggggac ggccgcggga ccgccgacgg ccagccgggc
     3721 gtcgcggccg cccgtctcgc gcaggaagcg ccagacatcg gcggcggccg catcgcgccc
     3781 ggctgcgtgc gccgcgcgca ccagcgcgag ggcgtcggcc ggctcgtgct ggacgcgcca
     3841 gttggcttgc gcaagccgca gcgccgcggc cggatcgccg cgcaggtcga gttcgtagcg
     3901 cgactgctcg cgtccgtggc tgcggtcgcc gcgcagcagg gcggcgccga agcgctcgcc
     3961 gagcaggcgc gtgtcgcggc gcgtgcgggc atcgcgcgcg ccgtaggccg cgcgttccgc
     4021 cagcgcgacg cgcagcagca ccggatcggc cgcgtcgtgg cccgtgagca gcgtcagcac
     4081 ctcgcccggc cggccggcgt cgagcagcca gtccgcgtag gcacacagcg tgtagacgtc
     4141 ggaattcgat cgcagcgcgg cggcgaacag cgggcccgcg gccgggtcgc cgcgccgctg
     4201 cgccagttcg gcgcgcatca gctgcagcca gccgggcgcg tcgccgggct cgtcggcgct
     4261 ggtcgaagcg gccaggcggg cgagcgtggc ggtggccgcg gcgtcgcgcc cctgcag
//
LOCUS       DATCRO010000151         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_125406, whole genome shotgun sequence.
ACCESSION   DATCRO010000151 DATCRO010000000
VERSION     DATCRO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125406"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /gene="serS"
                     /locus_tag="VIN75_04200"
     CDS             <1..1147
                     /gene="serS"
                     /locus_tag="VIN75_04200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8973395.1"
                     /translation="QAKRNALSKQIGALKAKGQDTSAVMAEVGGIGDELKASAERLEA
                     LQGELTTILMGLPNLPDESVPLGADETGNVEVRRWGTPATFDFAVKDHVDVGAPLGLD
                     PETGAKLSGSRFTVLRGGIARLHRALAQFMLDVQTGEHGYTELYTPYIVNREVLEGTG
                     QLPKFKEDMFWVLRGGDENQPEQYLISTSEISLTNTVRGEILKAEDLPVKLTAHSPCF
                     RSEAGSAGRDTRGFIRQHQFDKVEMVQIAHPEKSMEALEEMTRHAEAILQKLGLPYRV
                     MLLCGGDMGFGSTKTYDLEVWLPAQDTYREISSCSNMGAFQARRMQARFRNAAGKPEL
                     VHTLNGSGLAVGRTLVAVLENYQQADGSVTIPEVLRPYMGGVAVLKP"
     gene            1157..1789
                     /locus_tag="VIN75_04205"
     CDS             1157..1789
                     /locus_tag="VIN75_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY8973396.1"
                     /translation="MDYPSYDPKSAVTPLSEDELAALDELLAGLPSDGAMTLDGMDGY
                     LTALLVSPVPLSTMKTGDWLPVIWGGDQEPAPAPFRSNQQRKRSTVLVLRHLRAVQEA
                     LSGPPDAWEPIFSVAEVPGTHGDELADATDWCLGFLAATDLAPEAWAPLQSDPEVGPG
                     LATIAMLAGEIEPGDDAGDDAEDLDDPHVRDHLSRVAADVVSQLWARKSA"
     gene            1812..>4043
                     /locus_tag="VIN75_04210"
     CDS             1812..>4043
                     /locus_tag="VIN75_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973397.1"
                     /translation="MAAIPADDPLAGLDAPARDVLAALALGALARSRAWVVDALAMPA
                     LRTPGSVAPSQELVRHTLARLQKAGLANEDERRPGFWTLPLNVYPTLYAQLLMRAPQA
                     GLQQALAHADAYDAAGIGRQAHGRLPTLSAAIARVRLDALCGAGPLDIAQLLDRLPPG
                     LHDIPALLDTAIADCIAEPLFDRLHPALQADVLAGALDRLGASLHTGIALGPAFVRER
                     ARRLVDDASSPNAHGLRWSFAFDRLLADGAERRGPDWVASFDDLLAPTIESGEAGNLV
                     DAIRATHGFAAAPHPDSVPLATAASRRVIAQAVSAAALATQGRWSDAEPVFDGALAAA
                     RKLTGKRRGLLPPSVARSHVLALLAQQTPGHLDKALKFCLAEGGKREPVHDHPFGAIA
                     LAIQMRLGAAPRDMKAFRPYGSQWGYRGASRVVSPLDHDRWLMRAWLKEGSLAEGLPP
                     AEADSARKLKEAYESAGFDALAEQLGAALRVLGGEPAPSWFFVPAGLERWQVALAALR
                     ALGEPAAAAPAAATATPESRIVWLIEMGDHGAVAGLMPHEQKRGVRGWGQLKAVPLSR
                     LQRGATTLTPADAAIVRAIRQESFGRALRFDLAQAVGALFGHPHVAFSDAPEQFIELV
                     EAGPELEVLDEGERLRVRLQPALRVTPDAATPRWAGSEAEAKELEALRLVSIWRDGPQ
                     RARVIRLSASQKRVAQLLGPAGLDIPRQGAAQLQEVLTGLGSHFRIHADDAGAAQAAR
                     EVEA"
BASE COUNT          550 a         1472 c         1496 g          525 t
ORIGIN      
        1 gcaggccaag cgcaacgcgc tgtccaagca gatcggcgcg ctcaaggcca aggggcagga
       61 cacctccgcg gtgatggccg aggtgggcgg catcggcgac gagctcaagg ccagcgccga
      121 gcgcctggaa gcgctgcagg gcgagctcac cacgatcctg atgggcctgc ccaacctgcc
      181 cgacgagtcg gtgcccctcg gcgcggacga gacgggcaac gtggaagtgc gccgctgggg
      241 cacgccggcc acgttcgact tcgcggtgaa ggaccacgtc gacgtcggcg ccccgctggg
      301 cctcgacccc gagaccggcg ccaagctctc gggctcgcgc ttcaccgtgc tgcgcggcgg
      361 catcgcgcgc ctgcaccgcg cgctcgccca gttcatgctg gacgtgcaga ccggcgagca
      421 cggctacacc gagctgtaca cgccctacat cgtcaaccgc gaggtgctgg aaggcaccgg
      481 ccagttgccc aagttcaagg aagacatgtt ctgggtgctg cgcggcggcg acgagaacca
      541 gcccgagcaa tacctgatct cgacctcgga gatctcgctg accaacaccg tgcgcggcga
      601 gatcctgaag gccgaagacc tgccggtgaa gctgacggcg cacagcccgt gcttccgctc
      661 ggaggcgggc tcggccggcc gcgacacgcg cggcttcatc cgccagcacc agttcgacaa
      721 ggtcgagatg gtgcagatcg cgcatcccga gaaaagcatg gaagcgctcg aggagatgac
      781 gcgccacgcc gaggccatcc tgcagaagct gggcctgccg taccgcgtga tgctgctgtg
      841 cggcggcgac atgggcttcg gctcgaccaa gacctacgac ctcgaggtgt ggctgcccgc
      901 gcaggacacc taccgcgaga tcagctcgtg ctcgaacatg ggcgcgttcc aggcccgccg
      961 catgcaggcg cgcttccgca acgccgccgg caagcccgag ctcgtgcaca cgctcaacgg
     1021 ctcgggcctg gccgtgggcc gcacgctggt cgcggtgctg gagaactacc agcaggccga
     1081 cggatcggtg acgatccccg aggtgctgcg tccgtacatg ggcggcgtcg ccgtcctcaa
     1141 gccctgagag gccgggatgg actacccgtc gtatgacccg aagtcggccg tcacgccgct
     1201 gagcgaggac gagctcgcgg cgctcgacga gctgctcgcc ggcctgccct ccgacggcgc
     1261 gatgacgctg gacggcatgg acggctacct caccgcgctg ctcgtgagcc ccgtgccgct
     1321 gtcgacgatg aagaccggcg actggctgcc cgtgatctgg ggcggcgacc aggagcccgc
     1381 gccggcgccg ttccgcagca accagcagcg caagcgctcc accgtgctcg tgctgcgcca
     1441 cctgcgcgcg gtgcaggagg cgctgtccgg tccgccggac gcgtgggagc cgatcttcag
     1501 cgtggccgag gtgcccggca cgcacggcga cgaactggcc gacgcgaccg actggtgcct
     1561 gggcttcctg gccgccaccg acctcgcgcc cgaggcctgg gcgccgctgc agtccgatcc
     1621 cgaggtcggt ccaggcctgg cgaccatcgc gatgctggcc ggcgagatcg agcctggcga
     1681 cgacgcgggc gacgacgccg aggacctgga cgacccgcac gtgcgcgacc acctgagccg
     1741 cgtcgcggcc gacgtggtgt cgcagctctg ggcgcgcaag tccgcgtgag cctgtcgacg
     1801 cgcgcccgct gatggccgcg attcccgccg acgatccgct ggccgggctg gacgcgcccg
     1861 cgcgtgacgt gctcgccgcg ctcgcgctcg gcgcgctggc gcgctcgcgc gcgtgggtgg
     1921 tcgacgcact ggcgatgccc gcgctgcgca cgcccggcag cgtggcgccg tcgcaggagc
     1981 tggtgcgcca cacgctggcg cggctgcaga aggcgggcct ggccaacgag gacgagcgcc
     2041 gtccgggctt ctggacgctg ccgctgaacg tatacccgac actgtatgcg cagctgctga
     2101 tgcgcgcgcc gcaggccggc ctgcagcagg cgctggcgca cgccgacgcc tacgacgccg
     2161 cgggcatcgg ccgccaggcg cacggacgct tgccgacctt gtcggcggcc atcgccaggg
     2221 tgcgcctcga cgcgctgtgc ggcgccggcc cgctcgacat cgcgcagctg ctcgatcggc
     2281 tgccgcccgg cctgcacgac atccccgcgc tgctggacac cgccatcgcc gactgcatcg
     2341 ccgaaccgct gttcgaccgt ctgcatccgg cgctgcaggc cgacgtgctc gccggcgcac
     2401 tcgatcgcct gggcgcgtcg ctgcacacgg gcatcgcgct cggcccggcc ttcgtgcgtg
     2461 agcgggcgcg gcggctggtg gacgacgcgt cgagcccgaa cgcgcacggg ttgcgctggt
     2521 cgttcgcgtt cgatcggctg ctggccgatg gcgcggagcg gcgcggcccc gactgggtgg
     2581 cgtcgttcga cgatctgctg gcgccgacca tcgaatccgg ggaagccggc aacctcgtcg
     2641 atgcgattcg cgccacgcac ggcttcgccg ccgcgcccca tcccgacagc gtgccgctgg
     2701 cgacggcggc cagcaggcgg gtcatcgccc aggccgtcag cgcggcggcg ctcgccacgc
     2761 aaggccgctg gtcggatgcc gagccggtgt tcgatggcgc gcttgccgcc gcgcgcaagc
     2821 tcacgggcaa gcggcgcggg ctgctgccgc cgtccgtggc gcgatcccat gtgctcgcgc
     2881 tgctggcgca gcagacgcct gggcacctgg acaaggcgct gaagttctgc ctcgccgagg
     2941 gcggcaagcg cgagccggtg cacgaccacc cgttcggcgc gatcgcgctg gccatccaga
     3001 tgcgcctggg cgccgcaccg cgcgacatga aggcgttccg gccgtacggc agccagtggg
     3061 ggtatcgcgg cgcgtcgcgc gtcgtgtctc cgctggacca tgaccgctgg ctgatgcgcg
     3121 cgtggctcaa ggagggttcg ctcgccgagg gcctgccgcc ggccgaggcc gattccgcac
     3181 ggaagctcaa ggaggcctac gaatcggccg gcttcgacgc cctggccgag cagctggggg
     3241 ccgcgctgcg tgtgctgggc ggcgagccgg cgccgtcgtg gttcttcgtt ccggcgggac
     3301 tggagcgctg gcaggtggcg ctcgccgcgc tgcgcgcgct cggcgaaccg gctgcggccg
     3361 cgccggccgc cgcgaccgcg acgccggagt cgcgcatcgt ctggctcatc gagatgggcg
     3421 atcacggcgc cgtcgccggc ctgatgccgc acgagcagaa gcgcggcgtg cgcggctggg
     3481 gccagctcaa ggcggtgccg ctgagccggc tgcagcgcgg cgcgacgacg ctgacgccgg
     3541 ccgacgccgc gatcgtccgc gcgatccgcc aggagagctt cggccgcgcg ctgcgcttcg
     3601 acctcgcgca ggcggtgggc gcgttgttcg ggcatccgca cgtggcgttc agcgatgcgc
     3661 ccgagcagtt catcgagctg gtggaggccg gccccgagct ggaggtgctg gacgagggcg
     3721 aacgccttcg ggtgcgcctg cagcccgcgc tgcgcgtgac gcccgacgcc gccacgccgc
     3781 gctgggccgg cagcgaggcc gaggccaagg aactcgaggc gctgcgcctg gtctctatct
     3841 ggcgcgatgg accgcagcgc gcgcgagtca tccgcctgag cgcgtcgcag aagcgcgtgg
     3901 cgcagctgct ggggccggcg ggcctcgaca tcccgcgcca gggcgccgcg cagctgcagg
     3961 aggtgctcac ggggctcggc agccatttcc gcatccacgc cgacgacgcc ggcgccgcgc
     4021 aggccgcgcg cgaggtcgag gcc
//
LOCUS       DATCRO010000152         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_114199, whole genome shotgun sequence.
ACCESSION   DATCRO010000152 DATCRO010000000
VERSION     DATCRO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114199"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VIN75_04215"
     CDS             complement(<1..446)
                     /locus_tag="VIN75_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8973398.1"
                     /translation="MSSYSIATIPGDGIGKEVIPAGREVLEALAATTDRFRFEFEDFG
                     WGGDWYRAHGEMMPADGLDALRGKDAILFGSAGDPHIPDHITLWGLRLKICQGFDQYA
                     NVRPTRILPGIDAPLKRCTPRDLDWVIVRENSEGEYAGVGGRVHQG"
     gene            569..1498
                     /locus_tag="VIN75_04220"
     CDS             569..1498
                     /locus_tag="VIN75_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973399.1"
                     /translation="MAAPVQPADLGFFSTLAASGSLSAGARELGISTPAVSKRLAQME
                     SRLGVTLVNRTTRRMSLTPEGELYVAHARRILGEIDDMAQLLGGAKGEPQGLLRVNAT
                     LGFGRSHVAPLISKFARRHPQVEVQLQLSVNPPPLTDDAFDVCIRFGAPPEARVIARR
                     IASNRRLLCASPAYLARAGQPKVPNDLTRHRCIGIRQGDEAYGIWRLSSGRGTAQRTE
                     AVKTRGMLSTNDGEIAVNWALDGHGILMRAEWDIARYLRSGRLVQVLASYRTPDADIY
                     AVYPQRHQLSARVRTFVDFMAAALGKDDMPPRA"
     gene            1590..>2375
                     /locus_tag="VIN75_04225"
     CDS             1590..>2375
                     /locus_tag="VIN75_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY8973400.1"
                     /translation="MPSRNPQILPPAFVPPYLLSRLAESASPPLSARAVSTLAIDREH
                     RALRGLVPPAVVSNITGTPVRPARPDSPERAIHDAGHTTELPGRLVRTEGRPATGDIA
                     ADEAYEHLGATWRLYREVYERDSIDGAGMPLTGSVHYGRNYDNAFWNGAQMVFGDGDG
                     QVMNRFTIAVDIIGHELTHGVIDHEAALVYQGQPGALNESICDVFGVLVKQHVLKQTA
                     QQADWLIGAGLFMSTVKAKALRSMAEPGTAYDDPVIGKDPQPAH"
BASE COUNT          372 a          864 c          805 g          334 t
ORIGIN      
        1 tggccctggt gcacgcggcc gccgacgccg gcgtattcgc cctcggagtt ctcgcgcacg
       61 atcacccagt ccaggtccct gggcgtgcag cgcttcagcg gcgcgtcgat gccgggcagg
      121 atgcgcgtcg gccgcacgtt cgcgtactgg tcgaagccct ggcagatctt caggcgcagg
      181 ccccacagcg tgatgtggtc gggaatgtgc ggatcgcccg cggagccgaa aagaatcgcg
      241 tccttgccgc gcagcgcatc gaggccgtcg gccggcatca tctcgccgtg cgcgcggtac
      301 cagtcgccgc cccagccgaa gtcctcgaac tcgaagcgga agcggtcggt ggtggcggcc
      361 agcgcctcca gcacctcgcg gccggcgggg atcacttcct tgccgatgcc gtcgccggga
      421 atggtggcga tgctgtagct gctcatcgtc gatgtccttc ttcgtgtgta ggtgctctgt
      481 cgggctccca ctgtaggatc ggctgagtcg aacgattcac ggcggaagtg aatccatcgt
      541 taacctgaat tcaacaatcg gatcacccat ggcagccccg gtccagcccg ccgatctcgg
      601 cttcttctcc acgctcgcgg ccagcggcag cctgagcgcg ggggcgcgag aactcggcat
      661 cagcacgccc gcggtgagca agcggctggc gcagatggag tcgcgcctgg gcgtcacgct
      721 ggtgaaccgc accacgcggc gcatgagcct cacgcccgag ggcgagctct acgtggcgca
      781 cgcgcgccgc atcctcggcg agatcgacga catggcgcag ctgctcggcg gcgcgaaggg
      841 cgagccccag gggctgctgc gcgtgaacgc cacgctgggc ttcgggcgca gccacgtggc
      901 gccgctgatc tcgaagttcg cgcgcaggca tccgcaggtc gaggtgcagc tgcagctctc
      961 ggtgaacccg ccgccgctga ccgacgacgc cttcgacgtc tgcatccgct tcggcgcacc
     1021 gcccgaggcc cgcgtgatcg cgcggcgcat cgcgtcgaac cggcgcctgc tgtgcgcctc
     1081 gcccgcctac ctcgcgcgcg ccgggcagcc caaggtgccc aacgacctga cgcggcaccg
     1141 ctgcataggc atccgccagg gcgacgaggc ctacggcatc tggcgcctga gcagcgggcg
     1201 cggcaccgcg cagcgcaccg aggcggtgaa gacgcgcggc atgctcagca ccaacgacgg
     1261 cgagatcgcg gtcaactggg cgctcgacgg ccacggcatc ctgatgcgcg ccgagtggga
     1321 catcgcgcgc tacctgcgca gcggccggct cgtgcaggtg ctcgccagct accgcacgcc
     1381 cgatgccgac atctacgcgg tgtacccgca gcgccaccag ctgtcggcgc gcgtgcgaac
     1441 tttcgtcgac ttcatggccg ccgcgctcgg caaagacgac atgccgccca gggcttgagc
     1501 cgcgatccga caggcgccgc ctcgcgtttc tgctacggtg gtttcgaacc gatcgacacc
     1561 ccgatcgacc ttcgacgaaa ggcccgccca tgccgtcgcg caatccgcag atcctgccgc
     1621 ccgccttcgt gccgccgtac ctgctgagcc ggcttgccga aagcgccagc ccgccgctga
     1681 gcgcgcgcgc ggtgagcacg ctggccatcg accgcgaaca tcgcgcgctg cgcggcctgg
     1741 tgccgccggc ggtggtgtcg aacatcaccg gcacaccggt gcgccccgcg cggcccgatt
     1801 cgccagagcg cgccatccac gacgcgggcc acacgaccga gctgcccggc aggctggtgc
     1861 gcaccgaagg caggcccgcc accggcgaca tcgcggccga cgaggcctac gagcacctgg
     1921 gcgcgacctg gcggctgtac cgcgaggtgt acgagcgcga ctccatcgac ggcgcgggca
     1981 tgccgctgac cggcagcgtg cattacggca ggaactacga caacgccttc tggaacggcg
     2041 cgcagatggt gttcggcgat ggcgacggtc aggtcatgaa ccgcttcacc atcgcggtgg
     2101 acatcatcgg ccacgagctc acgcacggcg tgatcgacca tgaggccgcg ctcgtctacc
     2161 agggccagcc aggcgcgctc aacgaatcga tctgcgacgt gttcggcgtc ctggtgaagc
     2221 agcacgtgct gaagcagacc gcgcagcagg ccgactggct catcggcgcg ggcctcttca
     2281 tgtccaccgt gaaggccaag gcgctgcgct cgatggccga gcccggcacc gcctacgacg
     2341 acccggtgat cggcaaggac ccgcagcccg cgcac
//
LOCUS       DATCRO010000153         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1047911, whole genome shotgun sequence.
ACCESSION   DATCRO010000153 DATCRO010000000
VERSION     DATCRO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047911"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..778
                     /locus_tag="VIN75_04230"
     CDS             209..778
                     /locus_tag="VIN75_04230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8973401.1"
                     /translation="MATDKELSDFLKSVEKRAFKRTAFAVRDDDAALDIVQDSMIRLA
                     EKYVDRPAAELPLLFQRILSNATMDWFRRQKVRSAVVHNFSEFGGGGDDDGEFDILEV
                     LDNLEGTVGTESAADSVSRDQILQLIESEVAELPARQREAFLLRYWEELDVAETASVM
                     GCSEGSVKTHCSRAVHALAKALKAKGLAP"
     gene            775..1221
                     /locus_tag="VIN75_04235"
     CDS             775..1221
                     /locus_tag="VIN75_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEY8973402.1"
                     /translation="MTKNTTSTFSLPDRDGLEARFGVRVASMLGERAQSTAPDISERL
                     RFAREQALARAQAARRQEQLAAAPVVVSRGRTAALASPLGWWFKLGSAAPLALLVLGL
                     AGIAHVHDKAQIAAVAEVDAALLSDDLPPDAYTDPGFAEYLKTTRE"
     gene            1232..2023
                     /locus_tag="VIN75_04240"
     CDS             1232..2023
                     /locus_tag="VIN75_04240"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY8973403.1"
                     /translation="MARSRFSRLIESLAAAALVAAVGFGMSAAVAANGGAATAQAAKG
                     DVGGPAWNELSPAHRQVLAPLANDWNGLDSRSKERWIDVAGRFHRMPPEEQQRATQRM
                     GEWAHMSIQQRTQARTVFQETRGLSKEEREARWKAYQALPEEKKRELAAKRAAAPAST
                     ASNTAFARRHAPPAVDVVQPKNNLIGGSAASRAAAGSAPLLVEPKRPGVTTTLLTRRA
                     TPPAHQREGAPKIAVGPNAVDRTTLLPKHATVAAAPASAPASVRR"
     gene            2020..>2331
                     /locus_tag="VIN75_04245"
     CDS             2020..>2331
                     /locus_tag="VIN75_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8973404.1"
                     /translation="MNERTAAAASGAPLDPRLAAAPLVRRMAAFLYEGVLLFGVTFAT
                     GLVFAVALHQNNAMKLRSSLIGVEFVVVGLYFIVLWVRSGQTLPMKTWHLRLLTDDGA
                     PV"
BASE COUNT          360 a          870 c          771 g          330 t
ORIGIN      
        1 tcgcatttgt gcgaagcacc attattgcac cgatcccccg gctttagaac ggcatctgcc
       61 cccaattcgt gtcgaacaga ggcttcgcgc aatacgaagc cttcgaggcc gaacggcggg
      121 tgccgacacc ccgtcgcccg gctgtggcgc ggtgacataa tccgccgcgt cctccagacg
      181 gggcgcgccg ccgcttcagt tctccacctt ggccaccgac aaagaacttt cagacttcct
      241 caagagtgtc gagaagcgcg ccttcaagcg cacggccttc gccgtgcgcg acgatgacgc
      301 agcgctcgac atcgtgcagg attcgatgat ccgtctggcc gagaagtacg tcgaccggcc
      361 cgcggccgaa ctgccgctgc tgttccagcg catcctgtcc aacgccacga tggactggtt
      421 ccggcgccag aaggtgcgca gcgcggtcgt gcacaatttc tcggaattcg ggggtggcgg
      481 cgacgacgac ggcgagttcg acatcctgga agtgctggac aacctcgagg gaaccgtcgg
      541 caccgagagc gccgccgatt ccgtatcgcg agaccagatc cttcagctca tcgaaagcga
      601 agtcgccgag ctcccggcgc gtcaacgcga agcgttcctg ctgcgttact gggaggaact
      661 agatgttgca gagacggcct ccgtgatggg atgctcggag ggcagcgtca agacgcactg
      721 ctcgcgcgcc gtccacgcgc tggccaaggc gttgaaagcc aagggactcg caccatgacc
      781 aagaacacga cttcgacctt ttccctgccg gatcgcgacg gcctggaggc ccgcttcggc
      841 gtgcgcgtcg cttcgatgct tggcgagcgc gcccagtcca ccgcccccga catcagcgag
      901 cgcctgcgct tcgcccgcga gcaggccctg gcccgtgccc aggccgcgcg ccgccaggaa
      961 cagctggccg ccgcgccggt ggtggtcagc cgcggccgca ccgcagcgct ggccagcccg
     1021 ctgggctggt ggttcaagct cggctcggcc gccccgctgg cgctgctggt gctgggcctg
     1081 gccggcatcg cccacgtgca tgacaaggcg cagatcgccg ccgtcgccga ggtggacgcc
     1141 gccctgctgt cggacgacct gccgccggac gcctacacgg atccgggctt tgcggagtac
     1201 ctcaagacca cccgcgagtg acggaggagt cttggcgcgc tcccggtttt cccgcctgat
     1261 cgaatcgctc gccgccgcgg ccctggtcgc ggcggtgggc tttggcatgt ccgcggccgt
     1321 cgccgccaac ggcggcgcgg cgaccgccca ggcagccaag ggcgacgtcg gcggcccggc
     1381 atggaacgag ctctcgccgg cccatcgcca ggtgctcgcc cccctggcca acgactggaa
     1441 cggcctggat tcgcgcagca aggagcgctg gatcgacgtc gccgggcgct tccacaggat
     1501 gccgccggaa gagcagcagc gggccacgca gcgcatgggc gagtgggccc acatgtccat
     1561 ccagcagcgc acccaggcac gcacggtgtt ccaggagacg cgcggcctgt ccaaggaaga
     1621 gcgcgaggcg cgctggaagg cctaccaggc gctccccgag gaaaagaagc gcgagctcgc
     1681 cgccaagcgc gccgccgcgc cggccagcac ggcgtcgaac accgccttcg cccgccgcca
     1741 cgccccgccc gctgtggacg tcgtgcagcc caagaacaac ctgatcggcg gctcggccgc
     1801 ctctcgcgcg gccgccggat cggcgccgct gctcgtcgag cccaagcggc ccggcgtcac
     1861 cacaacgctg ctcacgcgcc gcgccacgcc gccggcgcac cagcgcgaag gcgcgcccaa
     1921 gatcgccgtc ggcccgaatg ccgtcgaccg cacgacgctg ctgcccaagc acgcgaccgt
     1981 ggccgccgcc ccggcgtcgg cgccggcgtc cgtgcgccga tgaacgaacg gacggcggcc
     2041 gccgcctccg gggccccgct cgatccccgg ctcgccgccg ccccgctcgt gcgccgcatg
     2101 gccgccttcc tctacgaagg cgtgctgctg ttcggcgtca ccttcgccac cggcctcgtg
     2161 ttcgccgtcg cgctgcacca gaacaacgcg atgaagctgc gttcgtcgct gatcggggtc
     2221 gagttcgtcg tggtcggcct gtacttcatc gtcctgtggg tgcgcagcgg ccagacgctg
     2281 ccgatgaaga cctggcacct gcgcctgctc accgacgacg gcgcgcccgt c
//
LOCUS       DATCRO010000154         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_597876, whole genome shotgun sequence.
ACCESSION   DATCRO010000154 DATCRO010000000
VERSION     DATCRO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597876"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..590
                     /gene="crcB"
                     /locus_tag="VIN75_04250"
     CDS             54..590
                     /gene="crcB"
                     /locus_tag="VIN75_04250"
                     /inference="COORDINATES: protein motif:HMM:NF010794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY8973405.1"
                     /translation="MHGSSSRLECRGHPGPRRARARGQRTLPPRASDARCRQERSARI
                     GGCREAPFPSHGAEHSASPVEHGAALRHFVNGASLRLVGSDWPLGTLFINVTGSILMG
                     VVAALFAFKLSLPPTLRLFIATGILGGYTTFSTFSLETALLMERGQSGFALLYVAGSV
                     LLGVGGLFTGMWAVRALT"
     gene            757..1416
                     /locus_tag="VIN75_04255"
     CDS             757..1416
                     /locus_tag="VIN75_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8973406.1"
                     /translation="MSLRSSCAALLAGAAWHACAAVHPPEPVFEPETASSDVRFAEKW
                     VRDHDDNHGRPYAIVDKKTAHIYVHAADGRLVGSSAVLTGLARGDESAPGIGQRPVNQ
                     IRPFERTTPAGRFDSQPGRTPTGEANVWIDYDTALAIHRLRPGRSQPRRQAALASTDP
                     AAHRQSYGCVVVPPAFFDGVVMGALGRARGVVYVLPEDRPVETIFQQTLADAAPRKRP
                     L"
     gene            1631..>3150
                     /locus_tag="VIN75_04260"
     CDS             1631..>3150
                     /locus_tag="VIN75_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8973407.1"
                     /translation="MSIPLLRKILQARRWLVPQSLATRASLATSIVFLAGVAVMASVS
                     LKTFDKQLSAVLSSEQDLLVGRIAENVDYRLELLQTALRDSAAHIDEHDLADVRSARE
                     ALERNDGLASIFDRSVFLFSDKGILLAERPYRPDRIGNDATWRPYIRQTLQTHRPVIS
                     EPFKTNAGDGNYVVVLTMPVLARDGKLIGLLTGSLGLTRPEMLGSVAKTRIGQTGYVS
                     IVTGDGKLIIDPDARRLSKPMYAPGVNALFDRALTGFEGSGSSADENGREAFISYHRI
                     KTAGWIVSAVYPKDEAYAYVGNLVRNFLLALAACALGILFAIWSLTRYLTQPLIALSR
                     HITAYTATDGRIAPLPGGSAAGSGEVHALTSAFNALTRRLNEREESLVSTMRQYQLIT
                     EGSTDLITRHARDGSIHFASPASLNVLGVEAAALLRTNIAQRVHPQDLPLLQSAFDAA
                     ASGDGATTVAYRARLANDEYVWLESALRSLSVGGQGDDILCLSRNIDQRKRMEDQL"
BASE COUNT          456 a         1186 c         1041 g          467 t
ORIGIN      
        1 aagtgacgca gcgcggcgcc gaagccgccg ccgacgaaga cgaggagata tttgtgcatg
       61 ggtcgagttc gcggttggag tgtcgtggac atcctgggcc gcggcgggct cgcgctcgtg
      121 gacaacgcac gctcccgccg cgggcatcgg acgcccggtg tcggcaggag cgatcagcca
      181 ggatcggcgg ttgtcgggaa gccccattcc catcccacgg ggcggagcat agcgcatcgc
      241 ctgtcgagca cggcgccgcg ctgcgtcact tcgtcaacgg cgcctcgctg cggctcgtgg
      301 gctccgactg gccgctgggc acgctgttca tcaacgtgac gggctcgatc ctgatgggcg
      361 tcgtcgccgc gctgttcgcg ttcaagctgt cgctgccccc gacgctgcgc ctgttcatcg
      421 ccaccggcat cctgggcggc tacacgacct tctcgacctt ctcgctcgag acggcgctgc
      481 tgatggagcg cggccagtcg ggcttcgcgc tgctgtacgt ggccggctcg gtcctgctgg
      541 gcgtgggcgg gctgttcacg ggcatgtggg cggtgcgcgc gctgacctga gaggacgccg
      601 cgcgggacgt gcccccgcgc cttcgcctcg cgtccaaaac cgggtcggat gcggcgctga
      661 tccgaagatc gacgcgcccg cgtccccgga aaattcggga cattccggcc tgcattcggg
      721 ccgctgcgaa ggcgctcggg cgcccgtccc ttccccatgt cgcttcgttc gtcgtgcgct
      781 gcgctcctgg ccggcgccgc gtggcacgcc tgtgccgcgg tccatccccc tgaaccggtg
      841 ttcgagcccg agacggcctc gagcgacgtc cgcttcgccg agaagtgggt tcgcgaccat
      901 gacgacaacc acggccggcc gtacgccatc gtcgacaaga agacggccca catctacgtc
      961 catgccgccg acggccgcct ggtcggcagt tcggccgtcc tgaccggcct tgcccgcggc
     1021 gacgagtcgg cgccgggcat cggccagcgt cccgtgaacc agatccgtcc cttcgaacgc
     1081 acgaccccgg ccggccgctt cgactcgcag ccgggccgca cgccgacggg cgaggccaac
     1141 gtctggatcg actacgacac cgcgctcgcc atccaccggc tgcgcccggg gcggtcgcag
     1201 cccaggcggc aggccgccct cgcctcgacc gatcccgccg cgcatcgcca gtcgtacggc
     1261 tgcgtggtgg tgccccccgc gttcttcgac ggggtcgtca tgggcgccct cggccgggcg
     1321 cgcggcgtcg tctatgtcct gccggaggac cgcccggtcg agacgatctt ccagcagacc
     1381 ctggccgacg ccgcaccgcg caagcggccg ctctagcgat cgcgctcgat cgcgcgccag
     1441 gcgcgcccgc gccgctgtag ggagatctcc agccccgcct gcgccggccc gccgaacagc
     1501 cgcctccggc ggacatttca cacacatcgc gcgctcgccg gctgctcgac cagaactccc
     1561 gacacctcca ggggtacagc gagcgccacc attcccgaat aggcgatgaa cccgcctcga
     1621 gagatccaac atgtccatcc ccctgctccg caagatcctg caagcgcgcc gctggctcgt
     1681 gccgcagagc ctggccacgc gcgcctccct ggcgacctcg atcgtcttcc tggcaggcgt
     1741 ggccgtgatg gcgtcggtct cgctcaagac cttcgacaag cagctcagcg cggtgctgtc
     1801 cagcgagcag gacctgctcg tcggccgcat cgccgagaac gtcgactacc gcctcgagct
     1861 gctgcagacc gcgctgcgcg acagcgcggc gcacatcgac gagcacgacc tggcggatgt
     1921 gcgcagcgcg cgcgaggcgc tcgagcgcaa cgacgggctg gcgtccatct tcgaccgctc
     1981 cgtcttcctg ttctcggaca aggggatcct gctggccgaa cggccgtacc gtcccgaccg
     2041 catcggcaac gacgcgacct ggcgtcccta catccgccag acgctgcaga cccaccggcc
     2101 cgtcatctcc gaaccgttca agaccaacgc cggcgacggc aactacgtcg tcgtgctgac
     2161 gatgcccgtg ctggcgcgcg acggcaagct gatcggcctg ctcaccggca gcctggggct
     2221 cacgcgtccg gagatgctgg gcagcgtcgc caagacccgc atcgggcaga cgggctacgt
     2281 gtccatcgtc acgggcgacg gcaagctgat catcgatcct gacgcgcgcc gcctgtccaa
     2341 gcccatgtac gcaccgggcg tcaacgcgct gttcgaccgc gcgctcaccg gcttcgaagg
     2401 gtcgggctcg agcgccgacg agaacggccg cgaggcgttc atcagctacc accgcatcaa
     2461 gacggcgggc tggatcgtct cggccgtcta ccccaaggac gaggcctatg cctacgtcgg
     2521 caacctcgtg cgcaacttcc tgctggcgct ggccgcgtgc gcgctgggca tcctgttcgc
     2581 gatctggtcg ctgacgcgct acctgacgca gccgctgatc gcgctgagcc gccacatcac
     2641 cgcctacacc gcgaccgacg gccgcatcgc cccgctgccg ggcggcagcg ccgcgggcag
     2701 cggcgaggtg catgcgctga cctcggcttt caacgcgctg acgcggcgcc tgaacgagcg
     2761 cgaggaatcg ctggtcagca cgatgcgcca gtaccagctc atcaccgagg gctcgaccga
     2821 cctgatcacg cgccacgcgc gcgatggctc gatccacttc gcctccccgg cctcgctcaa
     2881 cgtgctgggc gtcgaggcgg cggcgctgct gcgcaccaac atcgcgcagc gcgtgcatcc
     2941 gcaggacctg ccgctgctgc agtccgcctt cgacgcggcg gcgtcgggcg acggcgccac
     3001 gacggtcgcc taccgcgcgc ggctggcgaa cgacgaatac gtgtggctcg agagcgcgct
     3061 gcgctcgctg tcggtgggcg gccagggcga cgacatcctc tgcctgtcgc gcaacatcga
     3121 ccagcgcaag cgcatggagg accagctcca
//
LOCUS       DATCRO010000155         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1104402, whole genome shotgun sequence.
ACCESSION   DATCRO010000155 DATCRO010000000
VERSION     DATCRO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104402"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VIN75_04265"
     CDS             complement(<1..257)
                     /locus_tag="VIN75_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8973408.1"
                     /translation="MTTSTQIRNIAIIAHVDHGKTTLVDQLLRQSGTFAEHEKVVDTV
                     MDNNAIERERGITILAKNCAVSWKGTHINIVDTPGHADFGG"
     gene            complement(283..1275)
                     /gene="truB"
                     /locus_tag="VIN75_04270"
     CDS             complement(283..1275)
                     /gene="truB"
                     /locus_tag="VIN75_04270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8973409.1"
                     /translation="MTELQATAANDAAAPARAPRPARVRVQRRPVHGVLLLDKPLGLS
                     SNDALQKAKWLLRAEKAGHTGTLDPLATGLLPLCFGAATKFSQISLDSDKAYRAVLRL
                     GQTTRTGDAEGEVVRTRPVHVDRAALEAACARWTGPIRQVPPMYSALKHEGKALYEYA
                     RDGVQIERAARDVTIHTLDIVDWQHGNPETATIEVRCSKGTYVRTLAEDIGETLGCGA
                     HLVGLRRLASGPLTLESAITLEALEAMPEAERQACLLRPDSLLADWPAVRLEASEAAR
                     FLTGLRRRVAEPDRPHVRVYGPGQHALLGSAHITAGELIADRLLSPAEVQGLSN"
     gene            complement(1284..1688)
                     /gene="rbfA"
                     /locus_tag="VIN75_04275"
     CDS             complement(1284..1688)
                     /gene="rbfA"
                     /locus_tag="VIN75_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8973410.1"
                     /translation="MRHKQRSIPNRGFRVADQIQRDLAELIRELKDPRVGMITINSVE
                     VTPDYAHAKVFFSLLIGDPSESETALNEAAGFLRNGLFKRLAIHTVPTLHFHFDRTTE
                     RAAELNAMILQANATRAKEDDAKPDAEPKSGD"
     gene            complement(1769..>2453)
                     /locus_tag="VIN75_04280"
     CDS             complement(1769..>2453)
                     /locus_tag="VIN75_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY8973411.1"
                     /translation="YRDVKLAKQQAAKLENMFDQTGESAQTLSLIVKADVQGSQEALA
                     QSLVKLSTDEVKVQLVYAAVGGISESDVNLAIASKAVIIGFNVRADAGARKLAENNGV
                     DLRYYDIIYDAVDEVKAAMTGMLAPEQREEVIGAAEIRAIFTVSKTNIAGCMVTSGIV
                     RRSARVRLLRDNVVVYTGELESLRRMKDDVREVREGFECGLTIKNYNDVKEGDQLEFF
                     EIKEVARTL"
BASE COUNT          324 a          809 c          857 g          463 t
ORIGIN      
        1 tcgccgccga agtccgcgtg gcccggggtg tcgacgatgt tgatgtgcgt gcccttccag
       61 ctgaccgcgc agttcttggc caggatggtg atgccgcgct cgcgctcgat cgcgttgttg
      121 tccatcacgg tgtcgacgac cttctcgtgc tcggcgaagg tgccgctctg gcggagcagc
      181 tggtcgacga gcgtggtctt gccatggtcg acgtgggcga tgatggcgat gttgcggatc
      241 tgcgtgcttg tggtcatgac ggtctctgga gggatctggg gttcagttcg acaggccctg
      301 cacttcggcg gggctcagca ggcggtcggc gatcagttcg cccgccgtga tgtgcgcgct
      361 gcccagcagc gcatgttgtc cggggccgta cacgcggacg tgcgggcggt cgggttcggc
      421 cacgcgccgg cgcaggccgg tcaggaaacg cgccgcctcg ctggcctcga ggcgcacggc
      481 cggccagtcg gccagcaggg aatcgggtcg cagcaggcag gcctggcgct cggcctcggg
      541 catcgcctcg agcgcctcca gcgtgatcgc cgactcgagc gtcaacgggc cgctggcaag
      601 gcgtcgcagg ccgaccaggt gggcgccgca gcccagcgtc tcgccgatgt cctcggccag
      661 cgtccggacg taggtgccct tggaacagcg aacctcgatt gtcgccgtct ccgggtttcc
      721 atgctgccaa tccacgatgt ccagcgtgtg aatcgtcacg tcgcgcgcgg cacgctcgat
      781 ctgcacgccg tcgcgggcgt attcgtacag cgccttgccc tcgtgcttga gggccgagta
      841 catcggcggc acctggcgga tcgggccggt ccagcgggcg caggcggctt ccagcgcggc
      901 gcggtcgaca tggaccggac gggtccgcac gacctcgcct tcggcgtcgc cggtgcgcgt
      961 cgtctggccc aggcgcagca cggcgcggta ggccttgtcc gagtcgaggc tgatctggga
     1021 gaacttggtg gccgcgccga agcacagcgg cagcaggccg gtggccagcg ggtcgagcgt
     1081 gccggtgtgg ccggccttct cggcacggag cagccacttg gccttctgca atgcgtcgtt
     1141 ggacgacagg cccagcggct tgtccagcag cagcaccccg tgcaccggcc ggcgctgcac
     1201 gcgcacgcgc gcgggacgcg gcgcgcgcgc gggggcggcg gcgtcgttcg cggcggtggc
     1261 ttgcagctcg gtcatggtct ggctcagtct ccggacttcg gctctgcgtc cggcttggcg
     1321 tcgtcttcct tggcgcgcgt cgcattggcc tgcaggatca tggcgttgag ctcggccgcg
     1381 cgctcggtgg tgcggtcgaa gtggaagtgc agcgtgggca ccgtgtggat cgccaggcgc
     1441 ttgaacaggc cgttgcgcag gaagccggcg gcctcgttga gcgccgtctc gctctcggac
     1501 ggatcgccga tcagcagcga gaagaacacc ttcgcatgcg cgtagtcggg cgtgacctcc
     1561 accgagttga tggtgatcat gcccacgcgg ggatccttca gctcgcggat cagctcggcc
     1621 aggtcgcgct ggatctggtc ggccacgcgg aaaccgcggt tcggaatgct tcgttgcttg
     1681 tgtcgcatga tcgttctact gccctctcaa gtgaaaagcc ccgccaactt tctggcgggg
     1741 cttttgcaca ggtgaagtgc atcccgcctt aaagcgttcg ggcgacttcc ttgatctcga
     1801 agaactcgag ctggtcgcct tccttgacgt cgttgtagtt cttgatcgtc aggccgcact
     1861 cgaagccttc gcgcacttcg cgcacgtcgt ccttcatgcg gcgcagcgat tcgagctcgc
     1921 ccgtgtagac gaccacgttg tcccgcagca ggcgcacgcg cgcgctgcgg cgcacgatgc
     1981 ccgaggtgac catgcaaccg gcgatgttcg tcttggacac cgtgaagatc gcgcggatct
     2041 cggcggcacc gatgacttcc tcgcgctgtt ccggcgccag catgcccgtc atcgcggcct
     2101 tcacctcgtc gacggcgtca tagatgatgt cgtagtagcg caggtcgacg ccgttgttct
     2161 ccgcgagctt gcgcgcgccg gcgtccgcac ggacgttgaa gccgatgatg acggccttcg
     2221 acgcgatcgc caggttgacg tccgactcgc tgatgccgcc cacggccgcg tagaccagct
     2281 gcaccttgac ctcgtcggtc gacagcttga ccaggctctg cgccagcgcc tcctgcgagc
     2341 cctgcacgtc ggccttgacg atgaggctga gcgtctgcgc gctttcgccc gtctggtcga
     2401 acatgttctc cagcttggcg gcctgctgct tcgccagctt gacgtcgcgg tac
//
LOCUS       DATCRO010000156         5976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_800490, whole genome shotgun sequence.
ACCESSION   DATCRO010000156 DATCRO010000000
VERSION     DATCRO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800490"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VIN75_04285"
     CDS             complement(<1..364)
                     /locus_tag="VIN75_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY8973412.1"
                     /translation="MSTAYFTHADCRRHDMGRGHPECPARLDAIEDHFLATGLADVLA
                     RHDDAPVARLEDLAHAHTGGYLGELGEIFERVAESGELTSVDPDTAACPQTRQAALRA
                     AGAAVAATDLVLDGRAENA"
     gene            482..1408
                     /locus_tag="VIN75_04290"
     CDS             482..1408
                     /locus_tag="VIN75_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8973413.1"
                     /translation="MTSSLQARLTRLQGQISTIIVGKPAQIDDSIACLLAGGHLLIED
                     VPGVGKTTLAHVLAVSLGLSFSRVQFTADLMPSDLVGVSVYERSNESFAFHPGPVFAQ
                     VLLADEINRAGPKTQSALLEAMEEQQVSVDGRTRPLPQPFFVIATQNPGDQLGTYALP
                     ESQLDRFLMCITLGYPDAKSERELLAGQDRREAVARLAPVMTPAELIEAMAAVRQVHV
                     SDSLLDYLQALIAATRSGHWFSEGLSPRAGIGVLRLSRARALLAGRDFVSPDDLQAIL
                     PQAIAHRLIPVPGAGRGRREQVRAMIESIALP"
     gene            1417..2409
                     /locus_tag="VIN75_04295"
     CDS             1417..2409
                     /locus_tag="VIN75_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8973414.1"
                     /translation="MAQTTAPPAVRPSFLDVRGRWRAWWDGRREPRESQTLTQRNIYI
                     VPSRAGLMFGVVLMLLLVVSINYQLSLGFALTFLLAGSAAASMQMTHGSLRGLTLHLK
                     PLVPAFDGDAAVLEVVVTNPGGARLGVGFGLDLGVRPIPLAYAEIPAQGQVSVHLSWL
                     APRRGLHQLPVVRVESRYPFGLFRAWTIWRPAGQQWIYPRPESPTPPWPQVEASQGDD
                     RPAARTGSGTEFEGVRAYRRGDTLRQVVWKKAARTGELISRETAGAVQRELMLHWQQA
                     QGLDTEARLSRLAAWVVAADAAGHRWGLALPGAELAPDNGAPHRHAALRALARL"
     gene            2415..4571
                     /locus_tag="VIN75_04300"
     CDS             2415..4571
                     /locus_tag="VIN75_04300"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY8973415.1"
                     /translation="MTGRDIAIDQSAARAPSRWRAPSREARDTLFMIVLIGWTILPHL
                     LHLPPWVSGLSALVLGWRGWLAWRQSPLPGRMAVLALLGLAAVLTFTAEKTLVSKDAG
                     VTLLVVLMGLKTLELRARRDALVVFFLGFFLVLTTFLYSQTLLTALGTALSVWGWLTA
                     LTLAHIPAGRPTIRQAAGLAGRAALIGTPVMVALFLLFPRIGPLWPSADSAARTGLSD
                     RLELGSVADLANDDSIAFRVRFDGRPPPAGRLYFRGPVLTDTDGHEWRAQSLLDGRSR
                     RVGAARTLTVEERGQPTPYEMTLEPTQLTWLPMLEITPPPSTQQPADPSQGVQVLDGS
                     DVRPLPDLDLQWRTRRPISQRVRLRAVAGLDDRIVGATPSDLGANLRLPPRLFPRTRA
                     WAEQWRRDHPGRGGKPADPDAIVESLYRHIGTAGFTYTLAPGAYEQDPVDEFWLDRRV
                     GFCEHFATAFVVVLRSMGIPARVVTGYQGADPQLEDGYVIVRQSNAHAWVEYWNPARG
                     WVRADPTAAVAPDRIERGRAIQPAAGLVGSAFNAVSPGLLQRMRAWRETLDNRWNRWV
                     LGYNRNAQFDLLKKAGVGSPDTDDLGRLLMILISGTALAGTGLAWWDSHRRTPGQRLS
                     RRLASALRALQPHDVRVAPHESPGVVAAALRRRFGMPAEALAQSLDALERLRYGPAAG
                     TATPDWGSVRDAARALAAALAHRARAGDAAPLRASP"
     gene            4627..5832
                     /gene="mltB"
                     /locus_tag="VIN75_04305"
     CDS             4627..5832
                     /gene="mltB"
                     /locus_tag="VIN75_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY8973416.1"
                     /translation="MILFRAPELRLPSCTVLQHLVATIAVLAVAAITIPARAQADAAA
                     SAPAASHAKAKPKAARKHRKARPAVRDDDAPDSFVYGRRDDVLAFAAQVAEDRGLDRE
                     WVEDQLSRARYQPAVAKAIMPAPAGTAKNWAAYRARFIEQRRIAAGVQWWQAHAATLD
                     DAQRRYGVPPELVAGIVGVESFYGRMTGNFRVLDSLATLAFDFPKGRSDRSAFYRGEL
                     RAYLVWCALEGREADSVRGSFAGAIGWPQFMPSSLLKYAVDYDHDGHVDLAAGGDDVI
                     GSIASYMATFGWQRDLPIYFDVQPPADLEARARLVAVDIVPRLSVAELLDAGAQLPPE
                     ARDFPGKLGFVELQNGDAPPSYVAGTSNFYVVTRYNWSSYYAMAVIELGAAIKREMAL
                     APAARALPN"
BASE COUNT          742 a         2260 c         2131 g          843 t
ORIGIN      
        1 acgcgttctc ggcccggccg tcgagcacga gatcggtggc ggccaccgcg gcgccggcgg
       61 cgcgcagtgc ggcctggcgt gtctgcgggc aggcggcggt gtcgggatcg accgacgtca
      121 gctcgcccga ttcggccacg cgctcgaaga tctcgccgag ctcgccgaga tagccgccgg
      181 tgtgggcgtg ggccaggtcc tcgagccgcg cgacgggcgc gtcgtcgtgg cgcgcgagca
      241 cgtcggccag tccggtggcc aggaagtgat cctcgatcgc atcgagccgc gccgggcatt
      301 cgggatggcc ccggcccatg tcgtggcgac ggcagtcggc gtgggtgaag tacgcggtcg
      361 acatcatcgg cctcccgctt gcccggtcgc tgcgaccgcc tgtctcgaat ccctggtgtt
      421 catgtcgtcc ctgtccgccc gcgtgcacgc gttgtccctg ccgttcggct atcgttgagc
      481 catgacttca tcgctgcaag cgcgcctgac acggctgcaa ggccagatta gcacgatcat
      541 cgtcggcaag ccggcccaga tcgacgactc catcgcctgc ctgctggccg gcggccacct
      601 cctcatcgag gacgtccccg gcgtgggcaa gaccactctg gcgcacgtgc tcgcggtctc
      661 gctgggcctg agcttctcgc gcgtgcagtt caccgccgac ctgatgccca gtgacctggt
      721 gggcgtcagc gtctacgagc gcagcaacga gagcttcgcg ttccaccccg gcccggtgtt
      781 cgcgcaggtg ctgctggccg acgagatcaa ccgcgccggc ccgaagacgc agagcgcgct
      841 gctggaggcg atggaggagc agcaggtgag cgtcgacggc cggacccgac cgctgccgca
      901 gcccttcttc gtgatcgcca cgcagaaccc gggcgaccag ctgggcacct acgcgctgcc
      961 cgagtcgcag ctcgatcgct tcctgatgtg catcacgctc ggctaccccg acgcgaagtc
     1021 cgagcgcgaa ctgctggccg gccaggatcg ccgcgaggcg gtcgccaggc tggcgccggt
     1081 gatgacgccg gccgagctga tcgaggcgat ggccgcggtg cgccaggtgc acgtgtccga
     1141 ttcgctgctc gactacctgc aggcgctgat cgcggccacg cgctcgggcc actggttcag
     1201 cgagggcctg tcgccgcgcg cgggcatcgg cgtgctgcgg ctgtcgcgcg cccgtgccct
     1261 gctcgccgga cgcgacttcg tcagccccga cgacctgcag gccatcctcc cgcaggccat
     1321 cgcgcaccgc ctgatcccgg tgcccggcgc ggggcgcggc cggcgcgagc aggtgcgcgc
     1381 gatgatcgaa tcgatcgccc tgccctgatc ccggccatgg cacagacgac tgcgccgcct
     1441 gcggtccgcc cgtcgttcct cgacgtgcgg ggccgttggc gcgcctggtg ggacggccgc
     1501 cgcgagccgc gcgagagcca gacgctgacc cagcgcaaca tctacatcgt gccctcgcgc
     1561 gccggcctca tgttcggcgt cgtgctgatg ttgctgctgg tcgtgtcgat caactaccag
     1621 ctgagcctgg gcttcgccct caccttcctg ctcgcgggca gcgccgcggc gtcgatgcag
     1681 atgacgcacg gcagcctgcg cggcctcacg ctgcacctga agccgctggt gcccgcgttc
     1741 gacggcgacg ccgcggtgct cgaggtggtc gtcaccaacc ccggcggcgc gcgcctgggc
     1801 gtgggcttcg gcctcgacct gggcgtgcgc ccgatcccgc tcgcctacgc cgagatcccg
     1861 gcgcagggcc aggtctccgt gcacctgagc tggctcgcgc cgcgccgcgg cctgcaccag
     1921 ttgccggtgg tgcgcgtgga gtcgcgctac ccgttcggcc tgttccgcgc gtggacgatc
     1981 tggcggccgg ccggccagca gtggatctac ccgcgccccg agtcgccgac gccgccgtgg
     2041 ccgcaggtcg aggcgtcgca gggcgacgac cgccccgccg cgcgcaccgg ctcgggcacc
     2101 gagttcgagg gcgtgcgcgc ctaccgccgc ggcgacacgc tgcgccaggt ggtgtggaag
     2161 aaggccgccc gcaccggcga gctgatcagc cgcgagaccg ccggcgccgt gcagcgcgag
     2221 ctgatgctgc actggcagca ggcgcagggc ctggacaccg aggcgcggct gtcgcggctg
     2281 gccgcctggg tcgtcgcggc cgacgccgcc gggcatcgct ggggactggc cctgcccggc
     2341 gccgagctcg cgccggacaa cggcgccccg caccggcatg ccgcgctgcg cgcgctcgcc
     2401 aggctatgac cgcgatgacc ggccgcgaca tcgccatcga ccagtccgct gcgcgcgcgc
     2461 cgtcgcgctg gcgtgcgccc tcgcgcgagg cccgcgacac gctgttcatg atcgtgctga
     2521 tcggctggac catcctgccg cacctgctgc acctgccgcc gtgggtgtcg ggcctgtcgg
     2581 ccctggtgct gggctggcgc ggctggctcg cgtggcgcca gtcgcccctg cccggtcgca
     2641 tggcggtgct ggcgctgctg ggcctggccg ccgtgctcac gttcaccgcc gagaagacac
     2701 tggtcagcaa ggacgccggc gtcacgctgc tggtggtgct gatgggcctg aagacgctgg
     2761 agctgcgcgc gcggcgcgac gcgctggtcg tgttcttcct cggcttcttc ctggttctga
     2821 ccacgttcct gtactcgcag acgctgctca ccgcgctcgg caccgcgctg tcggtctggg
     2881 gctggctgac ggcattgacg ctggcccaca tccccgccgg gcggccgacg atccgccagg
     2941 cggcggggct ggccggacgc gccgcgctga tcggcacgcc ggtgatggtg gcgctgttcc
     3001 tgctgttccc gcgcatcggt ccgctgtggc cgtcggccga ttcggcggcg cgcacggggc
     3061 tgtccgatcg cctcgagctg ggcagcgtgg ccgacctcgc caacgacgac agcatcgcgt
     3121 tccgcgtgcg cttcgacggc cggccgccgc cggcgggccg gctgtacttc cgcgggccgg
     3181 tgctgaccga caccgacggc cacgaatggc gtgcccagtc ccttctggat ggccgctcgc
     3241 gccgcgtcgg cgcggcgcgc acgctcaccg tggaggagcg cggccagccc acgccctacg
     3301 aaatgacgct ggagccgacg cagctcacct ggctgccgat gctggagatc acgccgccgc
     3361 cgtcgacgca gcaacccgcc gatccgtcgc agggcgtgca ggtgctggac ggcagcgacg
     3421 tgcggccgct gcccgacctc gacctgcaat ggcgcacgcg ccgtccgatc tcccagcgcg
     3481 tgcggctgcg cgcggtcgcg gggctggatg accgcatcgt cggcgcgacg ccgtcggatc
     3541 tcggcgcgaa cctgcggctg cccccgcgct tgttcccgcg cacccgcgcc tgggccgagc
     3601 aatggcggcg cgaccacccg ggccgcggcg gcaagccggc cgacccggac gcgatcgtcg
     3661 agagcctgta ccgccacatc ggcacggccg gcttcaccta cacgctggcg cccggcgcgt
     3721 acgagcagga tccggtggac gagttctggc tcgatcgccg cgtcggcttc tgcgagcact
     3781 tcgccaccgc cttcgtcgtg gtgctgcgct ccatgggcat cccggcgcgc gtggtcacgg
     3841 gctaccaggg cgccgacccg caactcgagg acggctacgt gatcgtgcgc cagagcaatg
     3901 cccacgcgtg ggtcgagtac tggaacccgg cgcgcggctg ggtgcgggcc gacccgaccg
     3961 cggccgtcgc ccccgaccgc atcgagcgcg gccgcgcgat ccagccggcc gcgggcctcg
     4021 tcggcagcgc cttcaacgcg gtgagcccgg gcctgctgca gcgcatgcgc gcgtggcgcg
     4081 agacgctgga caaccgctgg aaccgctggg tgctgggcta caaccgcaat gcgcagttcg
     4141 acctgctcaa gaaggccggc gtcggctcgc ccgacaccga cgacctgggc cgcctgctga
     4201 tgatcctgat cagcggcacg gcgctggccg gcaccggact ggcctggtgg gactcgcacc
     4261 gccgcacgcc gggccagcgc ctgtcgcgcc ggctcgcgtc cgcgctgcgc gcgctgcagc
     4321 cacacgacgt gcgcgtcgcg ccgcacgagt cgccgggcgt cgtcgcggcc gcgctgcggc
     4381 ggcgcttcgg catgccggcc gaggcgctgg cgcaatcgct cgatgcgctc gagcgcctgc
     4441 gctacggccc cgcagccggc accgccacgc ccgactgggg cagcgtgcgc gacgccgcgc
     4501 gggccttggc ggccgcgctc gcgcaccgcg cccgcgcggg ggacgcggcg cccctgcgcg
     4561 caagccccta ggcacgcacg cgacacgtcg cgtcgtggac gtcccgcacg tcgctaaact
     4621 ggctcgatga tcctgttccg cgcgcccgag ctccgcctgc cctcttgcac cgtcctgcag
     4681 cacctcgtcg cgacgatcgc cgtcctcgcc gtcgcggcga tcacgatccc ggcgcgcgcg
     4741 caggccgacg cggcggcctc cgcgccggcg gcctcgcatg ccaaggccaa gcccaaggcc
     4801 gccaggaagc accggaaggc caggcccgcc gtgcgcgacg acgacgcgcc cgacagcttc
     4861 gtctacggcc ggcgcgacga cgtgctggcg ttcgctgcgc aggtcgcgga ggatcgcggg
     4921 ctcgaccgcg aatgggtcga ggaccaactg tcgcgggcgc gctaccagcc cgcggtcgcc
     4981 aaggccatca tgccggcccc ggccggcacc gcgaagaact gggccgccta ccgcgcccgc
     5041 ttcatcgagc agaggcgcat cgccgccggc gtgcagtggt ggcaggcgca cgcggccacg
     5101 ctcgacgacg cgcagcgacg ctacggcgtg ccgcccgagc tggtggccgg catcgtcggc
     5161 gtcgagagct tctacggccg catgaccggc aacttccgcg tgctcgattc gctggccacg
     5221 ctcgctttcg acttccccaa gggccgctcc gaccgcagcg cgttctaccg cggcgagctg
     5281 cgcgcctacc tcgtgtggtg cgccctcgag gggcgcgagg ccgacagcgt gcgcggctcg
     5341 ttcgcggggg ccatcggctg gccgcagttc atgcccagca gcctgctgaa gtacgcggtc
     5401 gactatgacc acgacggcca cgtcgacctg gccgccggcg gcgacgacgt gatcggcagc
     5461 atcgccagct acatggccac cttcggctgg cagcgcgacc tgccgatcta cttcgacgtg
     5521 cagccgccgg cggacctcga ggcgcgcgcc cgcctcgtcg ccgtcgacat cgtgccgcgc
     5581 ctgagcgtcg ccgaactgct ggacgccggc gcgcaactgc cgcccgaggc gcgcgacttt
     5641 ccgggcaagc tcggcttcgt cgagctgcag aacggcgacg cgccgcccag ctacgtggcc
     5701 ggcacgtcca acttctacgt ggtgacgcgc tacaactggt cgagctacta cgcgatggcc
     5761 gtgatcgagc tgggggcggc gatcaagcgc gagatggcgc tggcgccggc ggcgcgcgcg
     5821 ttgccgaact agcggctagg agtctgcgcg gcagaagccg cgcagactcc tagcggttga
     5881 atgcacggct cccccgagcc ccctccgaga gggagctcca gcatgtttga ctgcaccctc
     5941 cagcctccct ccgagaggga gactccagtc agcatg
//
LOCUS       DATCRO010000157         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_597892, whole genome shotgun sequence.
ACCESSION   DATCRO010000157 DATCRO010000000
VERSION     DATCRO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597892"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VIN75_04310"
     CDS             <1..1639
                     /locus_tag="VIN75_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8973417.1"
                     /translation="THGEIIMTREGHAVSQFAVAFYAPDSEQAGMLARAQEIEQLERE
                     SKAQALITDESRAALIRLEAAYTDAAQRLQSVRREASDAQTRAHELHVELLRLSSQAE
                     AATTRRTQLEDELAEIDAQDEGLIERRMTGEARFEELDLQLAETQERQADLEEAVIAA
                     ERKLSDAREQLRALERRAQEAQFDTRALNARQGELQRAIETAAQQVQANTQAGEQLTL
                     ELDSFNDQAAQAGLQDALALRSEREAALGAVRSEYDELSAQLRKADEQRLEFERSLQP
                     LRDAITKLQLEEQAAQLGGAQYMEQLAAAEVDLEALAKGIEEGGVKLHGLQTEIDRIG
                     REINALGAVNLAALDELVAARERKTFLDAQTEDLNDAIRTLEGAIHKIDLETRELLMK
                     TFNLVNEQFGRMFPSLFGGGQARLVMTGDEILDAGVQVMAQPPGKKNSTIHLLSGGEK
                     ALTAIALVFAIFMLNPAPFCLLDEVDAPLDDANTERYAKLVAEMSHKGTQFLFISHNK
                     IAMEMAEQLIGVTMQEQGVSRIVAVDMEAALSMAEAA"
     gene            1656..>2549
                     /locus_tag="VIN75_04315"
     CDS             1656..>2549
                     /locus_tag="VIN75_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8973418.1"
                     /translation="MHLNLTTALIALALLVLGGIVAQGWWKARRIARRPGGLSVVVDD
                     DLRREPTLGGDVAPPVDDELPAAAPPARADSRLAPARRAVRLDALIDAIATLSVDSPM
                     AGERVLPHLPGANRVGTKPFFIEGLNSETGEWETIAANARYSEFQAGVQMANRNGALN
                     EIEFSEFVQKLETFAQAIGASTDLPDMLESVARARELDAFASQHDAQLVALLRANSVA
                     WSVGYLQQVAQRHGFVPGAVPGRLVLPALEEGAPPVLVLGFDAQAALADDPGHAAVRE
                     MSLGLDVAQTEELAEPFAAWHG"
BASE COUNT          404 a          911 c          932 g          302 t
ORIGIN      
        1 cacgcacggc gagatcatca tgacccgcga gggccatgcc gtctcgcagt tcgccgtggc
       61 gttctacgcg cccgattccg agcaggccgg catgctggcg cgcgcgcagg agatcgagca
      121 gctggagcgc gagtcgaagg cccaggcgct gatcaccgac gaatcgcgcg cggcgctgat
      181 ccgcctggag gccgcgtaca ccgatgcggc gcagcgcctg cagtcggtgc ggcgcgaggc
      241 ctcggacgcg cagacgcgcg cgcacgagct gcacgtcgag ctgctgcgcc tgtcctcgca
      301 ggccgaggcg gcgacgacgc gccgcacgca gctggaggac gagctcgccg agatcgacgc
      361 ccaggacgag ggcctgatcg agcgccgcat gaccggcgag gcgcgcttcg aggaactcga
      421 cctgcagctg gccgagacgc aggagcgcca ggccgacctc gaggaggccg tcatcgccgc
      481 ggagcgcaag ctgtcggacg cgcgcgagca gctgcgcgcg ctggagcggc gcgcgcagga
      541 ggcgcagttc gacacccgcg cgctcaacgc ccgccagggc gagctgcagc gcgccatcga
      601 gacggccgcg cagcaggtgc aggccaacac gcaggcgggc gagcagctga cgctggagct
      661 cgacagcttc aacgaccagg ccgcgcaggc gggcctgcag gacgcgctgg cgctgcgttc
      721 ggagcgcgag gccgcgctcg gcgcggtgcg ctccgagtac gacgagctgt ccgcgcagct
      781 gcgcaaggcc gacgagcagc ggctcgagtt cgaacgcagc ctgcagccgc tgcgcgacgc
      841 catcaccaag ctgcagctcg aggagcaggc cgcgcagctg ggcggcgcgc agtacatgga
      901 gcagctggcg gccgccgagg tcgacctcga ggccctggcc aagggcatcg aggagggcgg
      961 cgtcaagctg cacggcctgc agaccgagat cgaccgcatc ggccgcgaga tcaacgcgct
     1021 cggcgcggtc aacctcgccg cgctcgacga gctggtcgcc gcgcgcgagc gcaagacctt
     1081 cctcgacgcg cagaccgaag acctgaacga cgccatccgc acgctcgagg gcgcgatcca
     1141 caagatcgac ctcgagacgc gcgagctgct gatgaagacg ttcaacctcg tcaacgagca
     1201 gttcggccgc atgttcccga gcctgttcgg cggcggccag gccaggctgg tgatgaccgg
     1261 cgacgagatc ctggacgccg gcgtgcaggt gatggcgcag cccccgggca agaagaactc
     1321 caccatccac ctgctctcgg gcggcgagaa ggcgctcacg gccatcgcgc tggtgttcgc
     1381 catcttcatg ctgaacccgg cgccgttctg cctgctggac gaggtcgatg cgccgctgga
     1441 cgacgccaac accgagcgct acgccaagct ggtggccgag atgtcgcaca agggcacgca
     1501 gttcctgttc atctcgcaca acaagatcgc gatggagatg gccgagcagc tgatcggcgt
     1561 cacgatgcag gagcagggcg tctcgcgcat cgtggcggtc gacatggagg ccgcgctgtc
     1621 catggccgag gccgcctgag ggaacccggg gccgcatgca cctgaacctc acgaccgccc
     1681 tgatcgcgct ggccctgctg gtgctgggcg gcatcgtcgc gcagggctgg tggaaggcgc
     1741 gccggatcgc gcgccggccc ggcggcctca gcgtcgtcgt cgacgacgac ctgcggcgcg
     1801 aacccacgct gggcggggac gtggcgccgc cggtggacga cgaactgccc gcggccgcgc
     1861 cgccggcgcg cgcggacagc cgcctcgcgc ccgcgcggcg cgccgtgcgc ctcgacgcgc
     1921 tcatcgacgc catcgccacg ttgagcgtcg acagcccgat ggccggcgaa cgcgtgctgc
     1981 cgcacctgcc cggcgccaac cgcgtcggca ccaagccgtt cttcatcgag ggcctcaaca
     2041 gcgagacggg cgagtgggag accatcgccg ccaacgcgcg ctacagcgag ttccaggccg
     2101 gggtgcagat ggccaaccgc aacggcgcgc tcaacgagat cgaattctcc gagttcgtgc
     2161 agaagctcga gaccttcgcg caggcgatcg gcgcgtccac cgacctgccc gacatgctcg
     2221 agagcgtggc ccgcgcgcgc gagctcgacg ccttcgccag ccagcacgac gcgcagctcg
     2281 tggcgctgct gcgcgccaac tcggtggcgt ggtcggtggg ctacctgcag caggtcgcgc
     2341 agcgccacgg cttcgtgccg ggcgcggtgc cgggccgtct cgtgctgccc gcgctggagg
     2401 agggcgcccc gccggtgctg gtgctgggct tcgacgccca ggccgcgctg gccgacgacc
     2461 cgggccatgc cgccgtgcgc gagatgtcgc tgggcctgga cgtggcgcag accgaggagc
     2521 tcgccgagcc cttcgccgcg tggcacggc
//
LOCUS       DATCRO010000158         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_57990, whole genome shotgun sequence.
ACCESSION   DATCRO010000158 DATCRO010000000
VERSION     DATCRO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57990"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..544)
                     /locus_tag="VIN75_04320"
     CDS             complement(146..544)
                     /locus_tag="VIN75_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973419.1"
                     /translation="MSQAPRPTDSPSASSKRVLLVDDNVDAVESMEILLQAFGYDVAT
                     AIHPELALGKLESFAPAAAVIDIGLPGMDGYQLAAEIRRRLGGRPMRLVAFTGYGSPD
                     DVARAHAAGFDAHLVKPVEIEQLLAALGGA"
     gene            774..2015
                     /locus_tag="VIN75_04325"
     CDS             774..2015
                     /locus_tag="VIN75_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8973420.1"
                     /translation="MSAVHVFPSDDPVEAAVEAEAAGEGGAPREKERLLLHMPIDVRS
                     TSMIVIASLLGLYFLRWAAPVVIPVLMGLLFSYALTPLVDRMVRLHVPRALGAAVVMA
                     ALVGGVAWGAWSLTDQTNNLIESLPGAAQKMRDTLHPLYPRQRDPSPSTIEQVQKAAA
                     TIAQATESSRPAPGVAKVQIVKPQFNIQDYVLTGTMRVAEAAAQALVVAFITYFLLCA
                     GDTFRRKLARIAGPNFAKRKITVQALDEITEQIQRYLLVQLLTSVVVGLATALAFWAL
                     GFNNAAVWGVLAGVMNLVPYVGTVVICGASAVVALLQFGTLQMALAVGSVSIVLHVIS
                     GYIITPWLTSRTSRLNAVVVFIGVLAWGWLWGVWGLLLGTPILMALKAICDRVEDLRA
                     IGELLGGDDKPGEGGSGTADA"
     gene            complement(2093..2848)
                     /locus_tag="VIN75_04330"
     CDS             complement(2093..2848)
                     /locus_tag="VIN75_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973421.1"
                     /translation="MQSSTVLRPLLAGALAALAFTLAPAARAASPVENYALAISWEPA
                     FCLQHSTKPECTSETSSRRDAHYFSLHGLWPQSGSYCGVSASQKSADEAGDWAALPAV
                     VLQPSTESLLQVDEPGTQSYLERHEWTEHGTCSGLSQQNFFAPTLAMLDGINASNLGS
                     TVSANVGGHVTLAQLQSAAALDYGTYAKIDIEYLCISSGGKSWLEEVRFHMRLPSPLP
                     TTVQSSYMAKPLNPASSSQLCKSTTPIYIRAVN"
     gene            complement(3081..3590)
                     /locus_tag="VIN75_04335"
     CDS             complement(3081..3590)
                     /locus_tag="VIN75_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8973422.1"
                     /translation="MPSHQPATHAASASQEPQDALQLLTAEHREVKAMFQQYDRLAEA
                     GGRGDERMLLASQICVALTLHAQVEEEILYPAARTALPHDEDIVDEAYVEHASAKSLV
                     AQIKTMTSDQPLFDAKVKVLGEYIDHHVKEEEGELFPKLRRTALDLDAMGERIAQRKK
                     QLMAMPEPS"
     gene            complement(3658..4956)
                     /locus_tag="VIN75_04340"
     CDS             complement(3658..4956)
                     /locus_tag="VIN75_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8973423.1"
                     /translation="MFATPVAHWFGRRGIHFSWVVMGIAFLTMLTSSAALGLPGAFLL
                     PLSKEFGWSTDQISGAIALRFVLYGLIGPFAAVLMERYGLRRIICSALLLIATGMALA
                     TQMTALWQLVTLWGLVLGVGSGLTALVLGATVANRWFEQRRGLVMGLLTASSATGQLI
                     FLPIAASLIQSHGWRLAITPVFTSCLVVAGLAFCLLRDRPNELGLAPFGADPAHADVA
                     HAAWRPNFLEPVRTLALASRSGAFWVLFGTFFVCGLSTSGLIQTHFITLCGDNGLGPV
                     PAASVLATIGACDFIGTIASGWLSDRYDVRKLLFWYYGLRGLSLLWLPHSTFTVVGLS
                     LFAVFYGLDWVATVPPTVRLTSAAFDKQRAPMIFGWIFAGHQLGGAVAAYGAGLTRTV
                     MRSYSPALYAAGLTCLLAAVAIFLLRRPAPAPLASPVRAG"
     gene            complement(4960..>5392)
                     /locus_tag="VIN75_04345"
     CDS             complement(4960..>5392)
                     /locus_tag="VIN75_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8973424.1"
                     /translation="SQADDAAGRALIVDKFLSAPSVKEVGLGCPSVSLAADVAREPAA
                     APIRSAYVAGLDALVDILAGVEDGPDEAARRRAAIADYATMAGALMLARATEGTPLSA
                     EFLAGARERLLRAPAPTSAPATAPAPAPARERATPRNTRKA"
BASE COUNT          792 a         1809 c         2014 g          777 t
ORIGIN      
        1 gtccatgctg cccgcaacgc tacgctggcc gactgcgctg gtcaatcgtg ggcgcttccc
       61 gtggcaatgt acttggcgcc gcgatgccgc gtcggtggtc gtcgaggtga cgctgagcat
      121 cccaggggtt tcgtgtcgcg gccggtcagg cgccgccgag cgcggccagc agctgctcga
      181 tctcgaccgg cttgaccagg tgcgcgtcga agccggccgc gtgggcgcgc gccacgtcgt
      241 ccgggctgcc atagccggtg aaggcgacca gtcgcatcgg ccggccgccc aggcggcgcc
      301 ggatctcggc ggcgagctgg tagccgtcca tgccgggcag gccgatgtcg atgacggccg
      361 ccgccggcgc gaacgactcg agcttgccca gcgcgagttc cgggtggatg gccgtggcga
      421 cgtcgtatcc gaacgcctgc agcaggatct ccatcgactc gacggcgtcg acgttgtcgt
      481 cgaccagcaa cacgcgcttg gacgatgcgg acggggaatc ggtggggcgg ggtgcttggg
      541 acatgcgacg gcgggcccgg ggacgatgga actgcgtcga ttatggcagt ggcccggggg
      601 tatcccccgt cgcgggccgt ccgcttgcct gctgtcctac gcgcggtgat gggctcggcc
      661 taccgggcgc tggccgcggc agctccacac tggggcgact cgacaatgat gacggagacg
      721 acgatggcgc agcaccgcgc agaccgatca cgcgagcccg cggaggccgg gcgatgagcg
      781 ccgtccacgt cttcccgagc gacgatccgg tggaggcggc cgtcgaggcg gaggccgcgg
      841 gcgaaggcgg tgcgccgcgg gagaaggagc gcttgctgct gcacatgccg atcgacgtgc
      901 gcagcacctc gatgatcgtc atcgcctcgc tgctggggct gtacttcctg cgctgggccg
      961 cgccggtcgt gatccccgtt ctgatgggcc tgctgttcag ctacgccctg acgccgctgg
     1021 tcgatcgcat ggtgcgcctg cacgttccgc gtgcgctcgg cgcggcggtc gtcatggccg
     1081 cactggtggg cggcgtcgcc tggggcgcct ggtcgctcac cgaccagacc aacaacctga
     1141 tcgaatcgct gcccggcgcc gcgcagaaga tgcgcgacac cctgcatccg ctgtatccgc
     1201 gccagcgcga cccgagcccc tcgaccatcg agcaggtgca gaaggccgcc gccaccatcg
     1261 cgcaggccac cgagagctcg aggcccgcgc ccggcgtggc caaggtgcag atcgtcaagc
     1321 cgcagttcaa catccaggac tacgtgctca cgggcacgat gcgcgtggcc gaggcggcgg
     1381 cgcaggcgct ggtggtggcc ttcatcacct acttccttct gtgcgcgggc gacaccttcc
     1441 ggcgcaagct cgcgcgcatc gccggcccca acttcgccaa gcgcaagatc accgtgcagg
     1501 cgctcgacga gatcaccgag cagatccagc gctacctgct ggtgcagctg ctgaccagcg
     1561 tcgtcgtggg cctggccacg gcgctggcgt tctgggcgct gggcttcaac aacgccgcgg
     1621 tctggggcgt gctcgccggc gtgatgaacc tcgtgcccta cgtcggcacc gtcgtgatct
     1681 gcggcgcgtc ggcggtggtg gcgctgctgc agttcggaac gctgcagatg gcgctggccg
     1741 tcggcagcgt gtccatcgtc ctgcacgtga tctcgggcta catcatcacg ccgtggctca
     1801 ccagccgcac cagccggctc aacgccgtcg tcgtgttcat cggcgtgctg gcctggggct
     1861 ggctgtgggg cgtctggggg ctgctgctgg gcaccccgat cctgatggcg ctcaaggcga
     1921 tctgcgaccg cgtggaggac ctgcgcgcga tcggcgagct gctgggcggc gacgacaagc
     1981 ccggcgaggg cggcagcggc accgccgacg cctgagaagc agggcggcga cggcgaagtg
     2041 aaggagaggg cggcgggccc ggcacgcgcc gccgccgggg ccggtcaggg catcagttca
     2101 cggcgcggat gtagatcggc gtggtgctct tgcaaagctg gctcgatgat gccggattca
     2161 gcggcttggc catgtacgac gactgcacgg tcgtcggcag ggggctgggc aggcgcatgt
     2221 ggaagcggac ctcctccagc cagctcttgc cgcccgagct gatgcacagg tactcgatgt
     2281 cgatcttggc gtaggtgccg tagtccagcg ccgcggctga ctgcagctgc gccagcgtca
     2341 cgtggccgcc gacgttggcc gacaccgtgg agccgaggtt gctggcattg atgccgtcga
     2401 gcatcgccag cgtcggcgcg aagaaattct gctgcgacag gcccgagcag gtgccgtgct
     2461 cggtccactc gtggcgttcg aggtaggact gcgtgcccgg ctcgtcgacc tgcagcagcg
     2521 attcggtgga cggctgcagg acgaccgcgg gcagcgccgc ccagtcgccg gcctcgtcgg
     2581 cggacttctg gctggcgctc acgccgcagt acgagccgct ctgcggccac agcccgtgca
     2641 gcgagaaata gtgggcgtca cgtcggctcg acgtttcgct cgtgcactcg ggcttggtcg
     2701 aatgctgcag gcagaacgcg ggctcccagc tgatggccag ggcgtagttc tcgacgggcg
     2761 aggcggcgcg ggcggcgggc gccagggtga aggcgagggc ggcgagggcg ccggcgagga
     2821 gcgggcgcag gacggtggaa gattgcatgc tcgtggttct ccaggtttgt gatgcggctc
     2881 gcgcgctcgt ggcccgcggc cgacccggat tcgagcatcg gctcgtgaca acagacaggg
     2941 ggaaacgccg gtctgccgca ggcagcctgc cgcgttgctt ttcgcgcgaa tcacctatgg
     3001 agaagcgggg cccgcgagcc cgcggccggc ataccccata ccgtgttcag ggtatggctg
     3061 cggccgcgcc gcgttcagct tcaggacggt tccggcatcg ccatcagctg cttctttcgc
     3121 tgcgcgatgc gctcacccat cgcgtccagg tcgagcgccg tccggcgcag cttcgggaac
     3181 agctcgcctt cttcttcctt gacgtggtgg tcgatgtact cgccaagcac cttgaccttg
     3241 gcgtcgaaca gcggctggtc gctggtcatc gtcttgatct gcgcgaccag gctcttggcg
     3301 ctggcgtgtt cgacgtaggc ctcgtcgacg atgtcctcgt catgcggcag cgccgtgcgc
     3361 gcggcggggt agaggatctc ttcctcgacc tgggcatgca gcgtcagcgc gacgcagatc
     3421 tggctcgcga gcagcatgcg ctcgtcgccg cgcccgccgg cctcggcgag cctgtcgtac
     3481 tgctggaaca tcgccttgac ctcgcgatgc tcggccgtca gcaactgcag ggcgtcctgg
     3541 ggctcctgcg atgcggatgc ggcatgggtg gcgggctgat gggatggcat ggctctctcc
     3601 ttgcgcgggt tgcgggccat cgaggcaacc cgcataccca tgcgcgcagg gcgcccgtca
     3661 gccggcgcgc accggggacg ccagcggcgc gggcgccgga cgcctgagca ggaagatggc
     3721 cacggccgcc agcaggcagg tcaggccggc cgcgtacagg gccgggctgt acgaccgcat
     3781 cacggtgcgg gtcaggccgg cgccgtaggc cgccacggcg ccgccgagct ggtggcccgc
     3841 gaagatccag ccgaagatca tcggcgcgcg ctgcttgtcg aacgcggcgc tggtcaggcg
     3901 cacggtgggc ggcacggtgg cgacccagtc gagcccgtag aacaccgcga acagcgacag
     3961 cccgaccacc gtgaacgtgg aatgcggcag ccacagcagc gacagcccgc gcaggccgta
     4021 gtaccagaac aggagcttgc gaacgtcgta gcggtcggac agccagcccg acgcgatcgt
     4081 gccgatgaag tcgcacgcgc cgatcgtggc gagcaccgac gcggcgggca ccgggccgag
     4141 cccgttgtcg ccgcacagcg tgatgaagtg cgtctggatc aggccgctgg tgctcaggcc
     4201 gcacacgaag aacgtgccga acagcaccca gaacgcgccg ctgcgagagg ccagggccag
     4261 cgtgcgcacc ggctcgagga agttcggccg ccacgcggcg tgcgccacgt cggcgtgcgc
     4321 gggatcggcg ccgaacggcg cgaggcccag ttcgttcggg cgatcgcgca gcaggcagaa
     4381 cgccaggccc gcgacgacca ggcagctcgt gaacaccggg gtgatggcca ggcgccagcc
     4441 gtggctctgg atcagcgagg cggcgatcgg caggaagatc agctgccctg tggcggaact
     4501 ggccgtgagc aggcccatca cgaggccgcg gcgctgctcg aaccaacggt tggcgacggt
     4561 ggcgcccagc accagcgcgg tcagccccga gccgacgccc agaacgagcc cccacagcgt
     4621 gacgagttgc cacagcgcgg tcatctgcgt ggccagcgcc atgccggtgg cgatcagcag
     4681 cagcgcgctg cagatgatgc ggcgcaggcc gtagcgctcc atcagcaccg ctgcgaacgg
     4741 gccgatgagg ccgtacagca cgaagcgcag cgcgatcgcg ccggagatct ggtcggtgct
     4801 ccagccgaac tccttgctga gcggcagcag gaaggcgccg ggcaggccca gcgccgccga
     4861 cgaggtgagc atcgtcagga acgcgatgcc catgacgacc caggagaagt ggatgccgcg
     4921 acggccgaac cagtgggcga cgggagtggc gaacatcgat caggccttcc gggtgttgcg
     4981 aggggtggcg cgctcgcgcg cgggggcggg cgccggcgcg gtcgcgggag cgctcgtggg
     5041 ggcgggcgcg cgcaggaggc gctcgcgggc gccggcgagg aactcggccg acagcggcgt
     5101 gccttcggtg gcgcgggcca gcatcagcgc gccggccatc gtggcgtagt cggcgatggc
     5161 cgcgcggcgc ctggcggcct cgtcggggcc gtcctcgacg ccggccagga tgtcgaccag
     5221 tgcgtcgagt cccgcgacgt aggccgaacg gatcggcgcg gcggccggct cgcgcgcgac
     5281 gtcggcggcc agcgagaccg acggacaacc gagcccgacc tccttgacgc tgggcgccga
     5341 caggaacttg tccacgatca gcgcgcgccc ggccgcgtcg tcggcctggc tc
//
LOCUS       DATCRO010000159         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_350274, whole genome shotgun sequence.
ACCESSION   DATCRO010000159 DATCRO010000000
VERSION     DATCRO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350274"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VIN75_04350"
     CDS             <1..364
                     /locus_tag="VIN75_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8973425.1"
                     /translation="GSTVTMAMVVVYTHLGHVPLWVVILINVVMFVGIFSRMIPFQAL
                     SSQVPEPTRRGAYNAISSSIQQVSGGVASAVAGLIVTSGPDGRIEHFSDVGYVVVGTT
                     LIGMWLMSRINRQVQSGT"
     gene            complement(361..585)
                     /locus_tag="VIN75_04355"
     CDS             complement(361..585)
                     /locus_tag="VIN75_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973426.1"
                     /translation="MTTPTRPTAFAELAFELHFEPLVDEQLPLTFPCDVDGRVDLDGL
                     CDRSRCDYLFAHTLIGRHFEMPVIRACAPA"
     gene            838..1011
                     /locus_tag="VIN75_04360"
     CDS             838..1011
                     /locus_tag="VIN75_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973427.1"
                     /translation="MTTTQISTTPADLLEQLDPQDDAFLSQFDDEALEQALKTVHWHA
                     KDVSQYLRATARG"
     gene            complement(1015..2388)
                     /locus_tag="VIN75_04365"
     CDS             complement(1015..2388)
                     /locus_tag="VIN75_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HEY8973428.1"
                     /translation="MPDRSASSVDVQTFLDEHPFSAFQWTIFALCFLIVLADGFDTAA
                     IGFIAPSLLKEWGLDKPALKGVLSAALFGLAAGALSSGPLADWLGRKKLLVASVLVFA
                     LACLASAFSQSLGQLTTLRFVTGLGLGAAMPNAVTLMSEYCPARLRATLTNAMFCGFP
                     LGAAFGGFLAAWMIPQFGWRSVLMLGGVAPLALAAAMALQLPESVRFMVARAQPAERI
                     RAALARISASAAQAASFRLSESRPAAEGRAGIALVLSRPYVVGSVMLWVAYFMGLVIF
                     YALINWMPILFKDAGLDPRSATLIAALFPLGGCGAVLAGWLMDRHDGNWIIAIGFAVT
                     ALLIWLIGQAAGQVGFLVLVVFLGGAFMNTAQSSLPALAAAYYPTAGRATGVSWMLGF
                     GRFGGIAGSLLVAELTRRQFTFAGIFSVVALPGLVAALALLVKRWAQPAQAADAGATP
                     AGGFAAH"
     gene            complement(2906..4384)
                     /locus_tag="VIN75_04370"
     CDS             complement(2906..4384)
                     /locus_tag="VIN75_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8973429.1"
                     /translation="MPQKLYEKLVVEILRSIDDGVLEAGDRLPSIREAASARHLSITT
                     VKRAYQALESQGAVEGRPKAGYFVRPTHGAPVVPLVRVSRPEPVSTVVDVGRLVLSTL
                     QRIHAHDALPLGAPYPDPALFSWRRVYQRTKGVARGLGEWRLGDDLPPGHPELIRQIA
                     RRHLRNGLDVDPAEIVVTAGATEAINLCLQAVARPGDTIAVESPTYYAMLQAIERMGM
                     RAIEVPTDPQRGIDVDVLARLLERQRIDACLTMPNIQNPLGFVMPDERKRALVDLAVR
                     HGMPLIEDAVYNELYYGAGPPTTLKAFDTTGIVLHCGSFSKSLSAGVRIGWALAGRYS
                     EQVAQLKFVNTHSTSALAQQGVAQFLAHEGWDHQLRGVRQVLEERGALMASMVRRFFP
                     EGTRMSQPRGGYLLWIELPGPADTLALHREALDRGISIAPGRVFSNGELYRNFLRLNY
                     SHAWTPRIEDAVKTLAQMACHASRPGAGDADARPTASLQLRE"
     gene            4505..>4709
                     /locus_tag="VIN75_04375"
     CDS             4505..>4709
                     /locus_tag="VIN75_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973430.1"
                     /translation="MSSPHAMRRHPDHLPPPPAVLTLGALRQVQLQLADLVTPVDACV
                     DVPLPRAAGRVLAGDVAAPDGGPP"
BASE COUNT          718 a         1682 c         1669 g          640 t
ORIGIN      
        1 cggcagcacg gtgacgatgg cgatggtggt ggtctacacg cacctcggcc acgtgccgct
       61 gtgggtggtg atcctgatca acgtcgtgat gttcgtgggc atcttctcgc ggatgatccc
      121 gttccaggcg ctgtcctccc aggtgccgga gccgacccgg cgcggcgcct acaacgcgat
      181 cagctcgtcg atccagcagg tctcgggcgg cgtcgcgtcg gcggtggcgg gcctcatcgt
      241 gacgtcgggc ccggacggcc gcatcgagca cttctccgac gtcggctacg tggtcgtggg
      301 caccacgttg atcgggatgt ggctgatgag ccgcatcaat cgccaggtcc agtccggaac
      361 ctaggcgggc gcgcaggccc ggatgacggg catctcgaag tgccggccga tgagcgtgtg
      421 cgcgaacagg tagtcgcagc gcgagcggtc gcacagcccg tcgaggtcga cgcggccgtc
      481 gacgtcgcag ggaaacgtga gcggcagctg ctcgtcgacg agcggttcga agtgcagttc
      541 gaaggcgagc tcggcgaacg cggtggggcg ggtcggggtg gtcatgctgg ctcctgggag
      601 tcccttgccc cgcccatgca agcggcggac cctgtcagcg gacatgccgt gccgctgtcg
      661 gcggtgtcgg gagttctcga acggcggaac ttgagaggcg ggatcccggc agatgggggc
      721 cggacgggcg tcttttgtag gactgcacgc acgcgacgct gcgataacgt ctggcttcgc
      781 tgcctcgagc tcgacgccaa gatgcagcca tcactccccc acacggtgga acacatcatg
      841 acgacgaccc agatcagcac gaccccggcc gacctcctgg aacaactcga cccccaggac
      901 gacgccttcc tgtcgcagtt cgacgacgag gccctcgagc aggcgctcaa gacggtccac
      961 tggcatgcca aggacgtcag ccagtacctg cgcgccacgg cgcgcggttg accctcagtg
     1021 ggcggcgaag ccgcccgcgg gcgtcgcccc ggcgtccgcc gcctgggccg gctgcgccca
     1081 gcgcttgacc agcagcgcca gcgcggccac caggcccggc agcgccacca cgctgaagat
     1141 gccggcgaac gtgaactgcc ggcgcgtgag ctccgccacg agcagcgagc ccgcgatgcc
     1201 gccgaagcgg ccgaacccca gcatccacga cacgcccgtc gctcgccccg ccgtcgggta
     1261 gtacgccgcc gccagcgccg gcagcgacga ctgcgccgtg ttcatgaacg cgccgcccag
     1321 gaacaccacc agcaccagga agccgacctg ccccgccgcc tgcccgatca gccagatcag
     1381 cagcgccgtg acggcgaagc cgatggcgat gatccagttg ccgtcgtggc ggtccatcag
     1441 ccagccggcc agcaccgcgc cgcagccgcc cagcgggaac agcgccgcga tcagcgtggc
     1501 cgaccgcggg tcgagcccgg cgtccttgaa caggatcggc atccagttga tcagcgcgta
     1561 gaagatcacc aggcccatga agtaggccac ccacagcatc accgagccga ccacgtacgg
     1621 gcgcgacagc accagcgcga tgccggctcg gccttcggcg gccgggcggc tctcgctcag
     1681 gcggaacgac gccgcctgcg ccgcgctcgc cgagatgcgt gccagcgccg cgcggatgcg
     1741 ctcggccggc tgcgcgcgcg ccaccatgaa gcgcaccgac tccggcaact gcagcgccat
     1801 cgccgcggcc agcgccagcg gcgccacgcc gcccagcatc agcacgctgc gccagccgaa
     1861 ctgcgggatc atccacgccg cgaggaagcc gccgaacgcc gcgccgagcg gaaagccgca
     1921 gaacatcgcg ttggtgagcg tggcgcgcag gcgcgccggg cagtattcgc tcatcagcgt
     1981 gacggcatta ggcatcgcgg cgcccaaccc gaggccggtg acgaagcgca gcgtggtcag
     2041 ctggccgagc gattgcgaga acgccgacgc gaggcacgcc aacgcgaaca cgagcaccga
     2101 ggccacgagc agcttcttgc gccccagcca gtcggccagc gggcccgagg acaacgcccc
     2161 cgccgccagt ccgaacagcg ccgcgctgag cacgcccttg agcgccggct tgtccaggcc
     2221 ccactccttg agcagcgacg gcgcgatgaa gccgatcgcc gccgtgtcga agccgtcggc
     2281 cagcacgatc aggaagcaca gcgcgaagat cgtccactgg aacgccgaga acgggtgctc
     2341 gtcgaggaag gtctggacgt cgacgctgga ggcggagcgg tcgggcatgg cgggtctcgc
     2401 gctacgttgg agagaccggc ccgcacgggc cggcgccctg gtttgcgcgc agtgtccccg
     2461 tgcgggcagg ggcagtcccg cggggtttcc ctcggatgtt cgattagtcc aagcgttggg
     2521 cgtttctgtg cattcaccgg cccgcgcgca caggtgtcgt cctgcgcgca ggatgacggc
     2581 ctggggcctc gcgcgggatg acccggctgg gcggacgatc cgctgccgcc cgtcgctcga
     2641 cgggcggccc tccccaggaa agaggtgcgg atcgggcaag ggttagcagc tcgcgtgcca
     2701 gaagctgtgt gcacatgcaa ccaatcgccg tgtcgcgtcc gccgtcgtgg tgctccccgg
     2761 cgtccggcgc gccggccgtc gcgaacgaaa gtccgtaaag ccgaaatttg tcacagcgcg
     2821 ggaggccgca aagtgtcgct acatggggtc gggtgacaaa acgcgtcgca tgcccgggtc
     2881 cgtatgcccg gcgtcgcgcc gcgattcatt cgcgaagctg aagcgaagcg gtcgggcgcg
     2941 cgtcggcgtc gcccgcgccg gggcggctgg cgtggcaggc catctgcgcg agcgtcttga
     3001 cggcgtcttc gatccggggc gtccacgcgt ggctgtagtt caggcgcagg aagttgcgat
     3061 acagctcgcc gttggaaaac acgcggcccg gcgcgatcga gatgccgcga tcgagcgcct
     3121 cgcggtgcag cgccagggtg tcggcgggac cgggcagctc gatccacaac aggtagccgc
     3181 cgcgcggctg cgacatgcgc gtgccctcgg ggaagaaacg ccgcaccatc gacgccatca
     3241 gtgcgccgcg ctcctccagc acctgccgga cgccgcgcag ctggtggtcc cagccctcgt
     3301 gcgcgaggaa ctgcgcgacg ccctgctgcg cgagcgccga cgtcgagtgc gtgttgacga
     3361 acttcagctg cgccacctgc tcgctgtagc gcccggccag cgcccagccg atgcgcacgc
     3421 ccgccgacag gctcttggag aacgagccgc agtgcaggac gatgccggtg gtgtcgaagg
     3481 ccttgagcgt ggtgggcggg ccggcgccgt agtacagctc gttgtagacc gcgtcctcga
     3541 tcagcggcat gccgtggcgc acggccaggt cgaccagcgc gcgcttgcgc tcgtcgggca
     3601 tcacgaagcc cagcgggttc tggatgttgg gcatcgtcag gcacgcgtcg atgcgctggc
     3661 gctcgagcag gcgcgcgagc acgtcgacgt cgatgccgcg ttgcgggtcg gtgggcacct
     3721 cgatcgcgcg catgcccatg cgctcgatgg cctgcagcat cgcgtagtac gtcggcgatt
     3781 cgaccgcgat cgtgtcgccc ggccgggcca cggcctgcag gcacaggttg atcgcctcgg
     3841 tggcgccggc ggtcaccacg atctcggccg gatcgacgtc gagcccgttg cgcaggtggc
     3901 ggcgcgcgat ctggcggatc agctccggat ggcccggcgg caggtcgtcg ccgagccgcc
     3961 attcgcccag gccgcgcgcc acgcccttgg tgcgctggta cacgcggcgc cacgagaaca
     4021 gcgccggatc gggatagggc gcacccagcg gcagcgcgtc gtgggcgtgg atgcgctgca
     4081 gcgtcgacag cacgagccgg ccgacgtcga cgacggtgga caccggctcg ggcctggaga
     4141 cccgcaccag cggcaccacg ggcgcgccat gcgtcggccg cacgaaatag cccgccttgg
     4201 gccggccttc gaccgcgccc tggctctcca gcgcctggta cgcgcgcttg acggtggtga
     4261 tgctcaggtg gcgcgcgctc gcggcctcgc ggatcgacgg cagccggtcg cccgcctcga
     4321 gcacgccgtc gtcgatcgaa cgcaggatct cgacgaccag cttctcgtac agcttctggg
     4381 gcatgcgcac ctcgccgaag gcggtggagg gaccacagcg accgctctgt gtgtgccgat
     4441 ggtagccccg gctgtgtctt ttggcggcgc gcggcggccc gatgtgcgac ggaacacgtc
     4501 ttgcgtgtcc tcgccccacg ccatgcgccg ccatcccgat catctccccc cgccgcccgc
     4561 cgtcctgacg ctgggcgccc tgcgccaggt gcagctgcag ctggccgatc tcgtcacccc
     4621 cgtcgacgcc tgcgtcgacg tcccgctccc tcgcgccgcc ggccgcgtgc tggcgggcga
     4681 cgtcgccgcg cccgacggcg ggccgcccc
//
LOCUS       DATCRO010000160         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_463053, whole genome shotgun sequence.
ACCESSION   DATCRO010000160 DATCRO010000000
VERSION     DATCRO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463053"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VIN75_04380"
     CDS             complement(<1..1603)
                     /locus_tag="VIN75_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8973431.1"
                     /translation="MDPARPLDDARRLDLLRQLGLLSAEADPALDRLCRIAATVTDAD
                     IAMITFLAGERQFVRSRVGTEATVVPASESLCLCALDGPGLVVIDDALADPRCSGNAH
                     VHGSGAIRAYAGMPLRFDGIVLGTLCVLHTQARRLGEEQRATLVDLAGMVEGLLRSRH
                     EQLLLEDQRRYAVELAAVLGESEAMLSEAQRLARLGSWDIDIATGLTAWSPSLYELFG
                     RDEALGPITADEFRDRMPPEDRPAFVAAVERAFHRFEKTRAEFRFPQADGQVRWISAV
                     SEPVRDARGRVLRLRGTLQDVTERRVSERRARESAERDRLLWQTSADVVVMVGEDNLI
                     RFCNPAIEPVLGWRPDEVVGQPLTMLQPERLREAHCRGFARYLASGERRLDWRAVEIV
                     GLHRDGREIPIEISFSDMQMDGQRIFGAFMRDVSQRVEQQRALQRSEERYRRIVQTAE
                     EGIWMVAPDATTTFVNPKMARMLGYEVSEMLGRSLYDFMDERAADEARENMRRREQGI
                     AEQHDFRLRCKDGSELWTAMSTSPIV"
     gene            1775..2314
                     /locus_tag="VIN75_04385"
     CDS             1775..2314
                     /locus_tag="VIN75_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8973432.1"
                     /translation="MTPHPIIRLTLAAMLAVGAALPALASGGGGGGGGGGGGMTTLPG
                     GKLSAVDPQYSQAVDAIQGDHYPQAIQLLEAYVASSPEHARDADAQNWLGYAYRKSGN
                     LDAAFLHYDKALAIDPEHRGAHEYMGEAYLMTGNIAQAEEHLKALDKLCFTPCEEYTK
                     LKRAVASWKAAHAQTVGTR"
     gene            2289..>2894
                     /locus_tag="VIN75_04390"
     CDS             2289..>2894
                     /locus_tag="VIN75_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEY8973433.1"
                     /translation="MRRRWAPGSLRRALAAAAGALLAGAGGLALLALAGGRSASAADG
                     AALAPDERAAIVAFGPWPPAAPHDARNPASGRPDGIAFGEALFHSPRLSPTGLRCASC
                     HEPWRHFADGRAVAQGVAAGSRNTPSLLDAATHRHYGWDGARDDLGAQSLRPLEDARE
                     MPATPAQLAAFVRADAATAARYAGVFGAAPGPDDARIRRDVG"
BASE COUNT          395 a         1080 c         1048 g          371 t
ORIGIN      
        1 cgacgatggg gctggtgctc atggccgtcc acagctcgct gccgtccttg cagcgcagcc
       61 ggaagtcgtg ctgctcggcg atgccctgct cgcgccggcg catgttctcg cgcgcctcgt
      121 cggcggcgcg ctcgtccatg aagtcgtaca gcgagcggcc cagcatctcg gacacctcgt
      181 agcccagcat gcgcgccatc ttggggttga cgaaggtggt ggtggcgtcg ggggccacca
      241 tccagatgcc ctcctcggcc gtctgcacga tgcgccggta gcgctcctcg ctgcgctgga
      301 gcgcgcgctg ctgctcgacg cgctgcgaga cgtcgcgcat gaacgcgccg aagatgcgct
      361 ggccgtccat ctgcatgtcg ctgaacgaga tctcgatggg gatctcgcgg ccgtcgcggt
      421 gcaggccgac gatctcgacg gcgcgccagt cgagccgccg ctccccgctt gccaggtagc
      481 gtgcgaagcc ccggcagtgc gcttcgcgca ggcgttcggg ctgcagcatc gtcagcggct
      541 ggccgacgac ctcgtccggc cgccagccca gcaccggctc gatggccggg ttgcagaagc
      601 ggatcaggtt gtcctcgccc accatgacga ccacgtccgc cgaggtctgc cacagcagcc
      661 ggtcgcgctc cgcgctctcg cgcgcgcggc gctcgctcac gcggcgctcg gtgacgtcct
      721 gcagcgtgcc gcgcaggcgc agcacccggc cgcgggcgtc gcgcacgggc tcgctgacgg
      781 cgctgatcca gcgcacctgg ccatcggcct gcgggaagcg gaactcggcc cgcgtcttct
      841 cgaaacggtg gaacgcgcgt tcgaccgcgg ccacgaaggc cgggcgatcc tccggcggca
      901 tgcggtcgcg gaattcgtcg gccgtgatcg ggccgagcgc ctcgtcgcgg ccgaacagct
      961 cgtacaacga cggcgaccag gcggtcaggc cggtggcgat gtcgatgtcc cagctgccca
     1021 ggcgcgccag ccgctgcgcc tcggacagca tcgcctcgga ctcgcccagc acggcggcga
     1081 gttcgaccgc ataacggcgc tggtcttcca gcagcaactg ctcgtggcgc gagcgcagca
     1141 ggccctccac catgccggcc aggtcgacca gcgtggcgcg ctgttcttca ccgagacggc
     1201 gggcctgcgt gtgcaggacg cacaaggtgc ccagcacgat gccgtcgaag cgcaggggca
     1261 tgccggcata ggcgcggatc gcgccggagc catgcacatg cgcgttgccg ctgcagcgcg
     1321 ggtcggccag cgcgtcgtcg atcaccacca ggccggggcc gtccagtgcg cacaggcaca
     1381 ggctctcgct ggccggcacg acggtcgcct cggtgccgac gcgcgagcgc acgaactggc
     1441 gttcgcctgc caggaaggtg atcatcgcga tgtcggcgtc cgtgaccgtc gccgcgatgc
     1501 gacacaggcg atcgagtgcc gggtcggcct cggcggacag caggcccagc tgccggagca
     1561 ggtcgaggcg gcgcgcgtcg tcgagaggtc gggcggggtc catccatcgg gccggagttg
     1621 tccttgcagg atagcgccga tgggggccgc cgcgcgcgcc gtcgctaccc aggacgaggg
     1681 agatggccgc gcagacgacg tcgccgtgaa ccttcgcacg ccgcgcgggc atccaccggc
     1741 ggacaacatc acttccacct tccaggagat tgccatgacc ccgcatccga tcatccgcct
     1801 gacgctggcc gccatgctgg ccgtgggcgc agcgctgccc gccctcgcct ccggaggcgg
     1861 cggcggaggc ggtggcgggg gcggcggcat gaccacgctg cccggaggca agctgtcggc
     1921 cgtcgacccg cagtacagcc aggcggtcga cgccatccag ggcgaccact atccgcaggc
     1981 gatccagctg ctcgaggcct atgtcgcgag cagccccgag cacgcgcgcg acgccgatgc
     2041 gcagaactgg ctcgggtacg cctatcgcaa gtcgggcaac ctcgacgctg cattcctgca
     2101 ctacgacaag gcgctggcca tcgaccccga gcaccggggc gcgcacgagt acatgggcga
     2161 ggcctacctg atgaccggca acatcgcgca ggccgaggag cacctgaagg cgctggacaa
     2221 gctgtgcttc acgccgtgcg aggagtacac caagctcaag cgcgccgtcg cgagctggaa
     2281 ggccgcgcat gcgcagacgg tgggcacccg gtagcctgcg gcgcgccctc gccgcggccg
     2341 ccggcgcgct gctggccggc gcgggcgggc tcgcgctgct cgcgctcgcg ggcggccgca
     2401 gtgcatcggc cgcggacggg gcagcgctcg cgccggacga gcgcgccgcc atcgtcgcgt
     2461 tcggtccgtg gccgccagcg gcgccgcacg acgcgcgcaa tccggcctcc ggccggccgg
     2521 acggcatcgc gttcggcgag gcgctgttcc acagcccgcg cctgtcgccc accggcctgc
     2581 gctgcgccag ctgccacgag ccctggcgcc acttcgccga cggccgcgcc gtggcgcagg
     2641 gcgtcgccgc gggctcgcgc aacacgccca gcctgctcga tgcggccacg caccggcact
     2701 acggctggga cggcgcgcgc gacgacctgg gcgcgcagag cctgcgcccg ctggaggacg
     2761 cccgcgagat gccggcgacg cccgcgcagc tcgccgcctt cgtgcgcgcc gacgcggcga
     2821 ccgcggcgcg ctacgccggc gtgttcggcg cggcgcccgg gcccgacgac gcccgcatcc
     2881 ggcgcgacgt cggc
//
LOCUS       DATCRO010000161         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1081922, whole genome shotgun sequence.
ACCESSION   DATCRO010000161 DATCRO010000000
VERSION     DATCRO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081922"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1218
                     /locus_tag="VIN75_04395"
     CDS             67..1218
                     /locus_tag="VIN75_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8973434.1"
                     /translation="MAFLNKNTALAVAALVACGTAAATEDYDLRYAPGYGAADMSAPF
                     EGGWVFQAHAYAYSGNVRGTSVQQTDLTGALSLALGQQLPPNSAGATTIIHTNEQINV
                     NGLLPRLSYMSSTTFLGATLGGTVLLPLVNKGSTASVTSVGPTTLSGVALGLPPANQA
                     GLIGLVNAGVTQGADALVAANSNKTWGPGDLEFSPILRWSTEATQTLFVLTTVAPTGD
                     YDKNRAANPSAGKFWTFRPAVQFSYIGDGWDMGTRVAYSYNTRNVETKYKSGSYVNVD
                     LALMKSISESTRIGLSGYGVEQLQKDNTRLSTAADYVATFGAANAAVAEAREAATLGE
                     KGRIIGVGPEIAYIHGAGDYLLEGRIMKEFGAESRPKGFTAFVTLSKPF"
     gene            1386..2009
                     /locus_tag="VIN75_04400"
     CDS             1386..2009
                     /locus_tag="VIN75_04400"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8973435.1"
                     /translation="MKMQTLRRLLSALVLVGAVSGAWANVDVSGVSLDENVTVGGVPL
                     HLNGAGYRKRGYFKIDVTAVYLPTKMTSLEAIENAPGVKRVQLTIQQDVTGSQASRYF
                     LIDFEASATPAEFAKLITEVSQVGDIYSSLPKIKKGDVVTMDWIPGKGLIATLNGNPL
                     TPHGAPSAYINSELLARVMLRMYIGGKTPAELHDNLLGLSTSMRDKK"
     gene            complement(2238..2636)
                     /gene="gloA"
                     /locus_tag="VIN75_04405"
     CDS             complement(2238..2636)
                     /gene="gloA"
                     /locus_tag="VIN75_04405"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY8973436.1"
                     /translation="MKFLHTMLRVGDLQRSIDFYTTHLGMTLRRTVDRPEQQYTLAYV
                     GYGENDDPRGEVGDVEVEFTYNYGVSSYDIGTAFGHLAIGVPDVGAVCDSVRNAGGKV
                     TREPGPVKGGTSVIAFVQDPDGYKIELIQV"
     gene            complement(2633..3703)
                     /locus_tag="VIN75_04410"
     CDS             complement(2633..3703)
                     /locus_tag="VIN75_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY8973437.1"
                     /translation="MEGLLDHPLREVLTAVGGIDRCVSEFIRITDQLMPERVFTRIVP
                     ELRQGGRTLAGVPVRPQLLGSDPACLADNAAALAALGPDGIDLNFGCPAKVVNRHGGG
                     ASLLVDPPLLGRIVEAVRRAVPPELPVTAKMRLGVDDASRAVECAQALAGAGASELVV
                     HARTKADGYRPPAFWERVPAIREAVSIPVVANGEIWTADDAERCRRITGCDALMLGRG
                     MVADPGLALAIRASRRSGVAPSLALPAHAMQAREDERGYVKASAGGPHSPDAYGAPPA
                     VPWNDLLPLIADFWRRVNVKVESRHRAGRLKQWLNLLRRRYPEAQRAYDELRLVTDPA
                     ALGGAMFANVAVTPQLDLADSP"
     gene            complement(3787..4983)
                     /locus_tag="VIN75_04415"
     CDS             complement(3787..4983)
                     /locus_tag="VIN75_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8973438.1"
                     /translation="MPVFGRSFLDHWWLDPSITYLNHGTVGATPRVVLEAQQAWQRRI
                     EAQPAAFLFRELMRLAPDEPGQPRLLLRQAADAVGAFLGARGDDLVFVDNATTGINAV
                     LRSLALAPGDEIVVLDQAYGAVVKTAEFVARAAGARVVVAETPFPVAGDATPACVAAV
                     ERVLTPRTRIAVIDHVVSDTALVLPIAAMTAACRARGVPVLVDGAHAPGAIALDIESI
                     GADWYVANLHKWAFAPRACGLLWVAPAHRAGLHPPVISWGLDVGLVQEFEWTGTRDPS
                     ALLAAPAGIAFMRDVLGVDAMRTWNHDLVWRMAHELAARWGQPFTTPEAMVGCMASVA
                     LPERISALGAAAAPALKDWLFHERRIEAPVLAIHGRLHVRLAAQVYNDEADFERLAQA
                     VDAWPA"
     gene            complement(5093..5317)
                     /locus_tag="VIN75_04420"
     CDS             complement(5093..5317)
                     /locus_tag="VIN75_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973439.1"
                     /translation="MIPAALPIAESLARYELRFQSLFHAGRALSFPCDRLGEVDWDAM
                     TDGARASFLRAQGSVGREWASPAVQLTDLH"
     gene            5527..6057
                     /locus_tag="VIN75_04425"
     CDS             5527..6057
                     /locus_tag="VIN75_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973440.1"
                     /translation="MNAPLIRLATVDDVPLLCGLGAATFRETYRPLSDPREVDEYADE
                     HFTPEKVAAWFRKPCARTLLAIAGGAAVGYAHLRQAKVPACVADRNAIELSRLYLLGT
                     AQGTGLGNAMMAAALAQAAELEARTVWLGAYDRNVRALAFYARHGFAQVGTHEFEFGG
                     RLYADPVLTRPVVLAP"
     gene            6105..6545
                     /locus_tag="VIN75_04430"
     CDS             6105..6545
                     /locus_tag="VIN75_04430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8973441.1"
                     /translation="MNSRTARLATALALAAGLATAAHADDDAALAAAAKEAGASVSST
                     GLVYRSLKDGTGASPKATDTVKVNYRGTFPDGKEFDASRGNPISFPLNRVIPCWTEGV
                     QKMKVGGKAKLTCPSAVAYGPRGAGGVIPPNATLVFEVELLAIN"
     gene            complement(6570..>6850)
                     /locus_tag="VIN75_04435"
     CDS             complement(6570..>6850)
                     /locus_tag="VIN75_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973442.1"
                     /translation="LDVGNSAGDDGVDRQLHEWAGEIVACELVSSCHGGLEAALHCVG
                     TGDCAHDDYRDVVLSKVPDTHRMVFDTMLAGMHARVGLTPGQADPDTR"
BASE COUNT         1063 a         2514 c         2358 g          915 t
ORIGIN      
        1 cgggtaatta gcgcgactag acttcgccca tcccatcgaa aactcgagta ccggagacaa
       61 actcacatgg cattcctgaa caagaacacc gccctggccg tcgccgccct ggtcgcctgc
      121 ggcacggccg ccgccacgga ggactacgac ctgcgctacg ccccaggcta cggcgcggcc
      181 gacatgtcgg ccccgttcga ggggggctgg gtgttccagg cgcacgccta cgcttacagc
      241 ggcaacgtcc gcggcacgtc cgtgcaacag acggacctga ccggcgcgct gtcgctcgcg
      301 ctgggccagc agctccctcc caattccgcc ggtgccacca cgatcatcca caccaacgag
      361 cagatcaacg tcaacggcct gctgccgcgc ctgagctaca tgagctccac caccttcctg
      421 ggcgcgaccc tgggcgggac ggtgctgctg ccgctggtca acaagggcag cacggccagc
      481 gtcaccagcg tcggccccac caccctgtcg ggcgtggccc tgggcctgcc gcccgccaac
      541 caggccgggc tcatcggcct cgtcaacgcg ggcgtgacgc aaggcgccga tgcgctggtc
      601 gcggccaact cgaacaagac ctggggcccg ggcgacctgg agttctcgcc gatcctgcgc
      661 tggagcaccg aagccacgca gaccctgttc gtcctgacga ccgtcgctcc caccggcgac
      721 tacgacaaga accgcgcggc caacccgagc gccggcaagt tctggacgtt ccgccccgcg
      781 gtgcagttca gctacatcgg cgacggctgg gacatgggca cgcgcgtcgc gtactcgtac
      841 aacacgcgca acgtcgagac caagtacaag agcggcagct acgtgaacgt ggacctcgcg
      901 ctgatgaagt cgatcagcga gtccacccgc atcggcctgt cgggctatgg ggtcgagcag
      961 ctgcagaagg acaacacccg cctgtccact gccgccgact acgtcgccac gttcggcgcc
     1021 gccaacgccg ccgtggccga ggctcgcgag gcggcgacgc tgggcgagaa gggccgcatc
     1081 atcggcgtcg gcccggagat cgcctacatc catggcgccg gcgactacct gctcgaaggc
     1141 cgcatcatga aggaattcgg cgcggagtcg cgtccgaagg gcttcacggc gttcgtcacg
     1201 ctgtccaagc cgttctgacg ccggccggaa gtcacggcgg gagcgccggc ggcagcgccg
     1261 gcactggagt cccgccgggc ttcgtccgat aaatggaagg cgacgcccgg cccacgaagc
     1321 cgaggcagcg ccgttccgcg cggcccgtca catcgccgcg ccccgaaagt gagggtgata
     1381 agaccatgaa gatgcaaacg ttgcgccggc tgctgtcggc cctggtgttg gtcggcgccg
     1441 tctccggcgc gtgggccaac gtcgatgtct ccggcgtctc gctggacgag aacgtcacgg
     1501 tcggcggcgt gccgctgcac ctgaacggcg ccggctaccg caagcgcggc tacttcaaga
     1561 tcgacgtcac cgcggtgtac ctgccgacga agatgacctc gctcgaggcc atcgagaacg
     1621 ccccgggcgt caagcgcgtg cagctgacca tccagcagga cgtcaccggc tcgcaggcct
     1681 cgcgttactt cctgatcgac ttcgaggcct ccgccacccc ggccgagttc gccaagctga
     1741 tcaccgaggt ctcgcaggtg ggggacatct acagctcgct gcccaagatc aagaagggtg
     1801 acgtcgtcac catggactgg attcccggca agggcctgat cgccacgctc aacggcaacc
     1861 cgctgacccc gcacggcgcg ccgtcggcct acatcaatag cgagctgctg gccagggtca
     1921 tgctgcgcat gtacatcggc ggcaagacgc ccgccgaact gcacgacaac ctgctgggcc
     1981 tgtcgaccag catgcgcgac aagaagtaat cgcccccgcc cgatctgccg gcctcgacgc
     2041 gtgggagcgc gtcggggcct tttcctttgg cagcgcccat cgcgcagggc ggggtcaggt
     2101 cttgaatcgt ccactcaaga cctgaccccg caacaatcaa gacctgaccc caacgcagag
     2161 gtggatcctg cgactgcgcg caggatgacg aggtggcggc atggcgaact gagcccgccc
     2221 ccttggcgcg caggcgctca gacctggatc agctcgatct tgtagccgtc cggatcctgc
     2281 acgaacgcga tgacggacgt gccgcccttc accggccccg gctcgcgggt gaccttgccg
     2341 ccggcgttgc gcacgctgtc gcacacggcg ccaacgtcgg gcacgccgat ggcgagatgg
     2401 ccgaatgcgg tgccgatgtc gtagctcgag acaccgtagt tgtaggtgaa ctcgacctcg
     2461 acgtcgccga cctcgccgcg cggatcgtcg ttctcgccgt agccgacgta ggccagcgtg
     2521 tactgctgct cggggcggtc gacggtgcgg cgcaacgtca tgcccaggtg cgtcgtgtag
     2581 aagtcgatcg agcgctgcag gtcgccgaca cgcagcatgg tgtggaggaa tttcatgggg
     2641 aatcggccag gtccaattgc ggggtcaccg ccacgttagc aaacatcgca ccacccagcg
     2701 cggcagggtc ggtgaccagg cgcagctcgt cgtacgcgcg ctgcgcctcg gggtagcggc
     2761 ggcgcagcag gttcagccac tgcttcaggc ggccggcgcg atggcgcgac tccaccttca
     2821 cgttcacgcg gcgccagaag tcggcgatca acggcagcag gtcgttccag gggacggccg
     2881 gcggcgcgcc gtaggcgtcg ggcgagtgcg ggccgcctgc cgaggccttg acgtagccgc
     2941 gctcgtcctc acgggcctgc atcgcgtgcg cgggcagcgc gagcgatggc gcgacacccg
     3001 agcgccggct tgcacggatc gccagcgcca ggccggggtc ggccaccatc ccgcggccca
     3061 gcatcagggc gtcgcagccg gtgatgcggc gacagcgctc ggcgtcgtcg gccgtccaga
     3121 tctcgccatt ggcgaccacg ggaatcgaca ccgcctcgcg gatggccggc acgcgttccc
     3181 agaaggccgg cggccggtag ccgtcggcct tcgtgcgcgc atgcacgacg agctcgctcg
     3241 cgcccgcgcc cgcgagcgcc tgcgcgcact cgactgcgcg cgaggcgtcg tccacgccca
     3301 ggcgcatctt ggccgtcacg ggcagctcag gcggcaccgc gcgccgcacg gcctcgacga
     3361 tgcgtccgag cagcggcgga tcgaccagca gcgacgcacc gccgccgtgc cggttgacca
     3421 ccttggccgg gcagccgaag ttcaggtcga tgccgtcggg cccgagggcg gcgagcgcgg
     3481 ccgcgttgtc ggccaggcag gccggatcgg agccgagcag ctgcgggcgc accggcacgc
     3541 cggccagcgt gcggccgccc tggcgcagct cgggcacgat gcgcgtgaag acgcgctcgg
     3601 gcatcagctg gtcggtgatg cggatgaact cggagacgca gcggtcgatg ccgccgacgg
     3661 cggtcagcac ctcgcgcaac gggtggtcga gcagcccttc catcggagcc agcagcagct
     3721 ggccaggcag cgacgcgcct tcgcgctcaa cgctccgggc ttcgccctca gcgcggccgg
     3781 gcgggctcat gccggccacg cgtccacggc ctgggcgaga cgctcgaagt cggcctcgtc
     3841 gttgtagacc tgggccgcga gccgcacgtg caggcggccg tggatggcca gcaccggggc
     3901 ctcgatgcgg cgctcgtgga acagccagtc cttcagcgcg ggcgccgcag cggcgccgag
     3961 cgccgagatg cgctcgggca gcgccacgct ggccatgcag ccgaccatcg cctcgggcgt
     4021 cgtgaacggc tggccccagc gcgcggcgag ctcgtgggcc atgcgccaca cgaggtcgtg
     4081 gttccatgtc cgcatcgcgt ccacgcccag cacatcgcgc atgaaggcga tgccggcggg
     4141 cgcggcgagc agggccgacg gatcgcgcgt gcccgtccac tcgaactcct gcacgaggcc
     4201 gacgtccagg ccccacgaga tcaccggcgg atgcagcccg gcacgatgcg ccggggccac
     4261 ccacagcagc ccgcaggcgc gcggcgcgaa ggcccacttg tgcaggttgg cgacgtacca
     4321 gtcggcgccg atcgactcga tgtcgagcgc gatggcgccg ggcgcatgcg cgccgtcgac
     4381 cagcaccggc acgccgcgcg cgcggcaggc ggccgtcatc gccgcgatcg gcagcacgag
     4441 cgcggtgtcg gaaacgacgt ggtcaattac cgcgatgcgc gtgcgcggcg tgagcacgcg
     4501 ctcgacggcc gcgacgcagg cgggcgtcgc gtcgcccgcc accgggaatg gcgtctcggc
     4561 gacgacgacg cgcgcgcccg cggcacgcgc cacgaactcg gcggtcttga cgaccgcgcc
     4621 gtaggcctgg tccagcacga cgatctcgtc gcccggcgcc agcgccagcg agcgcagcac
     4681 tgcgttgatg cccgtggtcg cgttgtcgac gaagacgagg tcgtcgccgc gcgcgcccag
     4741 gaacgcgccg accgcgtcgg ccgcctggcg cagcagcagg cggggctgcc cgggctcgtc
     4801 cggcgccagc cgcatcagct cgcggaacag gaacgcggcc ggctgcgcct cgatgcgtcg
     4861 ctgccaggcc tgctgcgcct ccagcaccac gcgcggcgtg gcgccgacgg tgccatggtt
     4921 caggtaggtg atcgacggat cgagccacca gtggtccagg aaggagcggc cgaagacggg
     4981 cacggaaagt cggggcggat ggcagatgcg ccattctcgc tcgccgtccg acacgagctg
     5041 ccggggccgg ttggccccag ggccggcccg ccctgcggcc cgcgccgcgg cttcagtgca
     5101 ggtcggtcag ttgcaccgcc ggcgacgccc attcacgccc gacgctgcct tgcgcacgca
     5161 ggaagctggc gcgggcgccg tcggtcatcg cgtcccagtc cacctcgccc aggcggtcgc
     5221 acgggaacga cagcgcgcgg ccggcgtgga acagcgactg gaagcgcagt tcgtagcggg
     5281 cgagcgactc ggcgatgggc agcgcagcgg ggatcatgtt ggcctcgggt gttgcgggtg
     5341 aggactgtgc gtgggaccag gcccagcgtg aaaccagtgt gcgcgccgcc gcgccagcac
     5401 aatatcagcg cgcttccatg ccgggcctag tccccgcgcc cgtgcgcctg tagtcgcggc
     5461 ggacgacgcc tgtcagcgcg cgtctgacaa acttcgcctc ctgtcccgct ccgacccgat
     5521 ccgcccgtga acgccccgct catccgcctc gccaccgtcg acgacgtccc cctgctctgc
     5581 ggcctgggcg ccgccacctt ccgcgagacc taccgcccgc tgagcgaccc gcgcgaggtc
     5641 gacgagtacg ccgacgagca cttcacgccg gagaaggtgg ccgcctggtt ccgcaagccc
     5701 tgcgcgcgca cgctgctcgc gatcgccggc ggcgcggccg tcggctacgc ccacctgcgc
     5761 caggccaagg ttccggcctg cgtggccgac cgcaacgcga tcgagctgtc gcggctgtac
     5821 ctgctgggca ccgcgcaggg cacgggcctg ggcaacgcga tgatggccgc cgcgctggcg
     5881 caggccgcgg agctcgaggc ccgtaccgtc tggctgggcg cctacgaccg caacgtgcgc
     5941 gcgctggcgt tctacgcgcg gcacggtttc gcgcaggtcg gcacgcacga gttcgaattc
     6001 ggggggcgcc tgtatgccga ccccgtcctg acgcgccccg tcgtcctcgc gccctgagcc
     6061 gcgactatca ttcgcgcctt ccaaaacatt tcggagatgt cccgatgaac tcccgcaccg
     6121 cccgcctggc caccgccctg gcgctcgccg ccggcttggc caccgccgcg cacgccgacg
     6181 acgacgccgc gctcgccgcc gccgccaagg aagccggcgc cagcgtgtcc tccaccggcc
     6241 tcgtctaccg gtcgctcaag gacggcacgg gggccagtcc gaaggccacc gacacggtca
     6301 aggtgaacta ccgcggcacc ttccccgacg gcaaggaatt cgacgcctcg cgcggcaacc
     6361 cgatctcgtt cccgctgaac cgcgtgatcc cgtgctggac ggaaggcgtg cagaagatga
     6421 aggtcggcgg caaggccaag ctgacctgcc cgtcggccgt cgcgtacggc ccgcgcggcg
     6481 ccggcggcgt catcccgccg aacgcgacgc tcgtgttcga ggtcgagctg ctggcgatca
     6541 actgaaaatt ccggcccgga cggtcggccc tacctggtgt ccgggtcggc ctggcccggc
     6601 gtcaggccca cgcgcgcgtg catgcccgca agcatcgtgt cgaagaccat gcggtgcgtg
     6661 tcgggcacct tcgagagcac gacgtcgcga tagtcgtcgt gcgcgcaatc gccggtgccc
     6721 acgcaatgca gcgcggcctc caggccgccg tggcagctgc tcaccagctc gcacgcgacg
     6781 atctccccgg cccattcgtg caactgccgg tcgacaccgt cgtcgcccgc cgaattgccc
     6841 acgtcgagga
//
LOCUS       DATCRO010000162         5882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_496867, whole genome shotgun sequence.
ACCESSION   DATCRO010000162 DATCRO010000000
VERSION     DATCRO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496867"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /gene="acpP"
                     /locus_tag="VIN75_04440"
     CDS             <1..151
                     /gene="acpP"
                     /locus_tag="VIN75_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8973443.1"
                     /translation="ADLGADSLDTVELVMALEDEFSIEIPDEEAEKITTVQLAIDYAK
                     AHAKA"
     gene            157..1401
                     /gene="fabF"
                     /locus_tag="VIN75_04445"
     CDS             157..1401
                     /gene="fabF"
                     /locus_tag="VIN75_04445"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8973444.1"
                     /translation="MSRRRVVVTGLGLVCPVGNTVAEGWANIVAGKSGIATITKFDAT
                     PFACHFAGEVKGFNVEDYIPGKDARHMDTFIHYGVAAALQAIKDAGLKTGDQLTEEEG
                     DRIGVLMGAGIGGLPEIEHTQTEYANRGPRRISPFFVPASIINMISGHISIYCGYRGP
                     NLGIVTACTTGLHSIGMAGRLIESGDADVMIAGGAESTVSPLGIGGFASARALSTRND
                     DPATASRPWDKDRDGFVLGEGAGALVLEEYEHAKARGATIYAELAGFGMSGDAYHMTA
                     PNVDGPKRSMLAALNNAGINGDQVDYLNAHGTSTPLGDLNETNAIKKAFGDAAGKLVV
                     SSTKSMTGHLLGGAGGIESVFTVLAIRDQVAPPTINLVNQDPECDLDYCANTARKMKI
                     DVAVKNNFGFGGTNGTLVFRRV"
     gene            1425..1937
                     /locus_tag="VIN75_04450"
     CDS             1425..1937
                     /locus_tag="VIN75_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973445.1"
                     /translation="MRAPPPVEYPSALGRAWRAALSLLVGASVAVPLAWGLPYFAAHW
                     GSRQPDALLDGLADPRIRGALALWTGAMAVAAAWLWQRGQAAGERTLRWDGQDWVLVS
                     AAAGRAEQRGDAELTLDLGPWMLVRFLPYAGLGGATWLPLSAGLDLARWAALRGALWN
                     WRAGAGDARP"
     gene            2002..2610
                     /gene="rpoE"
                     /locus_tag="VIN75_04455"
     CDS             2002..2610
                     /gene="rpoE"
                     /locus_tag="VIN75_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY8973446.1"
                     /translation="MLNPDPDAALVERVKQGDVRAFEMLVVKYQRRIERLIGRMVRDV
                     DLVSDIAQETFIRAYRALPQFRAESAFYTWLYRIAVNTAKKALVDMKRDPVTTLTALQ
                     GSDEDDETYRPGLEPTDGETPEALLASKEIAATVNAAIASLSEDLRQAITLREIEGLS
                     YEEISDVMNCPIGTVRSRIFRAREAIAERLRPLLDTREGNRW"
     gene            2709..3371
                     /locus_tag="VIN75_04460"
     CDS             2709..3371
                     /locus_tag="VIN75_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEY8973447.1"
                     /translation="MSQPDSDRSSSESSRRILSALADGDATDGEAARAFQAWRDDPDA
                     RAAWHSYQLIGDVLRSEDLACEPKADEAFLVALRARLADEPVVLAPQPRAAEADVAAP
                     AVANAGAAARHRARWQGPVAMAAGFLVVLGGLNIVRPFGHGPDASVASAGAASGPLMA
                     SAASSVSPIVATGLQTPAQTKAAADQLAPYLAAHRQSAMNAPFQMPGNDLRNVNLVQP
                     AQ"
     gene            3371..4564
                     /locus_tag="VIN75_04465"
     CDS             3371..4564
                     /locus_tag="VIN75_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEY8973448.1"
                     /translation="MAALLSLELPLPAPAARARRALGCVTPARVASARLAARRAMLPR
                     VAGMRGTLRSVTRFSGALVCAAALTTLSARALAQSLRTPAPPLAASSGESAGDLRAWL
                     VRIHDAAVRSNYVGTIVNSTGTSVVSSRVGHFFDGHGTLERIDALDGEPRSMLRINDE
                     VRTLWPRYRVAVIEPADPRAAFPALVSGNEKHIPDFYELHVQPAQRIAGHDADRLLLK
                     SRDDSRFDRVIWADHATGLLLRVDVQLAGRTLESSGFSDVNIGVKPDAAAVYNELHRA
                     DGFRIVRPAIEHTKLDTEGWSMQASLLPPGFMALDCVRRALDVPGDTAGQAKVLQAIW
                     SDGITHVSLFIEPFQPQRHRSEGLTVIGATHTLTRRVNDMWLTAVGDVPPTALDQFVR
                     ALEHK"
     gene            4633..>5882
                     /locus_tag="VIN75_04470"
     CDS             4633..>5882
                     /locus_tag="VIN75_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8973449.1"
                     /translation="MSKPNAPFAARATLAFATVLAAALPTLSRAEATPTPVPVQAPET
                     AVRGLPDFTGLVETVGPAVVNIRTNIKPKAAAGDQSLQAQMQELIEEYIRRHGGRGGR
                     NLPPGGNDAPEDDDQPEQGYIGSGFLLSPDGYVMTNAHVVEGADEVTVTLTDKREFKA
                     RIIGSDQRTDVALLKIDSTGLPFVKVGSVDRLKVGEWVIAIGSPFGLESTVTAGIVSA
                     KQRETGDYLQLLQTDVAINPGNSGGPLINMRGEVVGINSQILSPSGTSAGISLAIPID
                     EAIRVSDQLRASGHVTRGRIGVQIGIVNKQVAEAVGLGKPVGAMVQSVQAGLPAEKAG
                     IEAGDIITKVQGKPVDRYSDLPRLVGMVKPGEKVQLQVFHDGKYKDVTADVVDFGPET
                     PPTVAAGPDPEAIANTKATLGLKVV"
BASE COUNT          911 a         2165 c         2011 g          795 t
ORIGIN      
        1 ggccgacctg ggcgcggact cgctcgacac cgtcgaactg gtgatggcgc tggaagacga
       61 attcagcatc gagatcccgg acgaagaggc cgagaagatc acgacggtgc agctcgccat
      121 cgactacgcc aaggcccacg ccaaggcttg agcaagatga gtcgtcgtcg cgtcgtcgtc
      181 accggactgg gtctggtctg ccccgtgggc aacacggtgg ctgaaggctg ggccaacatc
      241 gtggccggga aatccgggat cgccacgatc accaagttcg atgccacccc gttcgcctgt
      301 catttcgcgg gcgaggtgaa gggtttcaat gtcgaggact acatccccgg caaggacgcc
      361 cgccacatgg ataccttcat ccactacggc gtggcggcgg cgttgcaggc gatcaaggac
      421 gccggcctca agaccggcga ccagctcacc gaggaagagg gcgaccgcat cggcgtcctg
      481 atgggtgccg gcatcggcgg cctgccggag atcgagcaca cgcagaccga gtacgcgaac
      541 cgcggcccgc gccgcatctc gccgttcttc gtgccggcct cgatcatcaa catgatctcg
      601 ggccacatct cgatctactg cggctaccgc ggccccaacc tgggcatcgt gacggcctgc
      661 acgacgggcc tgcactcgat cggcatggcc ggccgcctga tcgaatcggg tgacgcggac
      721 gtgatgatcg ccggcggcgc ggagtccacc gtgtcgccgc tgggcatcgg cggcttcgcc
      781 tccgcccgcg cgctgtccac gcgcaacgac gatccggcca cggcctcgcg cccgtgggac
      841 aaggatcgcg acggcttcgt cctgggcgag ggcgccggcg cgctcgtgct cgaggaatac
      901 gagcacgcca aggcgcgtgg cgccacgatc tatgccgagc tcgcgggctt cggcatgagc
      961 ggcgatgcgt accacatgac cgcccccaac gtcgacggcc ccaagcgctc gatgctggcg
     1021 gcgctgaaca acgcgggcat caacggcgac caggtcgact acctgaacgc gcacggcacg
     1081 tccacgccgc tgggcgacct gaacgagacc aacgccatca agaaggcgtt cggcgacgcc
     1141 gccgggaagc tggtcgtcag ctcgaccaag tcgatgaccg gccacctgct gggcggcgcc
     1201 ggcggcatcg agtcggtgtt caccgtcctg gccatccgtg accaggtcgc cccgccgacg
     1261 atcaacctgg tcaaccagga tcccgagtgc gatctcgact actgcgccaa cacggcgcgc
     1321 aagatgaaga tcgacgtcgc ggtgaagaac aacttcggct tcggcggcac caacggaacc
     1381 ctggtgttcc gcagggtctg agccccgcgc tcaaccggcg agccatgcgc gcgccgcccc
     1441 ccgtcgagta tccgagcgcg ctgggccgtg cctggcgcgc cgccctgtcg ctgctggtcg
     1501 gcgcatcggt ggccgtgccg ctcgcctggg gcctgccgta cttcgcggcg cactggggca
     1561 gccgccagcc tgatgcgctg ctcgacggtc tcgccgatcc ccggatccgg ggcgcgctcg
     1621 cgctgtggac cggggcgatg gccgtcgcgg ccgcctggtt gtggcagcgc ggccaggccg
     1681 ccggcgagcg cacgctgcgc tgggacgggc aggactgggt gctggtgtcg gccgccgccg
     1741 gccgcgccga gcagcgcggc gacgccgagc tgacgctcga cctcggcccc tggatgctgg
     1801 tgcgcttcct gccgtacgcc ggcctgggcg gcgccacctg gctgccgctg tcggccgggc
     1861 ttgacctggc ccgctgggcc gcgctgcgcg gcgcgctgtg gaactggcgc gccggcgccg
     1921 gggatgcacg tccatgagcg cgcacggccg ctccgaagcg ctcatcccga ggtgcgcagc
     1981 acggaggatc gtcaaatgac gatgctcaac ccggatccgg acgccgcgct ggtcgagcgc
     2041 gtcaagcagg gcgacgtgcg ggccttcgag atgctggtcg tcaagtacca gcgccgcatc
     2101 gaacgcctga tcggccggat ggtgcgcgac gtcgacctgg tgtccgacat cgcccaggaa
     2161 accttcatcc gcgcctaccg ggcgctgccg cagttccgtg ccgagagcgc gttctacacc
     2221 tggctctacc ggatcgccgt caacaccgcc aagaaggcgc tggtcgacat gaagcgcgat
     2281 cccgtgacga cgctcacggc gctgcaaggc tcggacgaag acgatgaaac ttaccggccc
     2341 ggattggaac caacggacgg cgagacgccc gaggccctgc tcgccagcaa ggaaatcgcc
     2401 gccaccgtca acgcggccat tgcctcgctg tcggaggatc tgcgccaggc catcaccctg
     2461 cgcgagatcg agggcctcag ctacgaagag atttcggacg tcatgaactg ccccatcggt
     2521 accgtgcgct cgcgcatctt ccgcgcccga gaggcgatcg cggaacgcct gcgcccgctg
     2581 ctcgataccc gagagggcaa tcgctggtga gcgcacactc agccgacaag acgacgccat
     2641 gcccagtttt atttgcactc cagatgactt ccgcccctgg cgaaccgatg atcccttgcg
     2701 aggtttgaat gtcacagcct gatagcgatc gttcttcttc cgagtccagc cgccgcatcc
     2761 tgtccgcgct ggccgacggc gacgccaccg acggcgaagc cgcgcgggcc ttccaggcct
     2821 ggcgcgacga cccggacgcc cgcgccgcct ggcacagcta ccagctgatc ggcgacgtgc
     2881 tgcgcagcga ggacctggcc tgcgaaccca aggccgacga agcgttcctg gtggcgctgc
     2941 gcgcgcgcct ggccgacgag ccggtcgtcc tcgcgccgca gccgcgcgcc gcggaggccg
     3001 acgtcgcggc gccggccgtg gccaatgccg gcgcggcggc gcgccaccgc gcccgctggc
     3061 aaggcccggt ggcgatggcc gccggcttcc tggtcgtcct tggcggcctg aacatcgtgc
     3121 gcccgttcgg ccacggcccg gacgcctcgg tggcgtcggc cggcgcggcc tcggggccgc
     3181 tgatggccag cgcggcctcg agcgtctcgc ccatcgtcgc caccggcctg cagacgcccg
     3241 ctcagaccaa ggcggcggcc gaccagctgg ctccgtacct cgcggcgcac cgccagtcgg
     3301 cgatgaacgc tccgttccag atgccgggca acgacctgcg caacgtcaac ctggtgcagc
     3361 ccgcgcagtg atggcggcgc tgctgtccct ggagctgccg ctgcccgcgc ccgccgcccg
     3421 cgcgcgtcgg gcgctcgggt gcgtcacgcc cgcgcgcgtg gcgtcggcgc gcctggccgc
     3481 gcggcgcgcc atgctgccgc gcgtggccgg catgcgtggc acgctgcgca gcgtgacgcg
     3541 gttctccggc gcgctggtgt gcgccgcggc gctgaccacg ctcagcgcgc gtgcgctggc
     3601 gcaatccctg cgcacgcccg cgccgccgct ggcggcctcc agcggcgaga gcgccggcga
     3661 cctgcgggcc tggctcgtgc gcatccacga cgccgccgtg cgcagcaact acgtcggcac
     3721 catcgtcaac agcaccggca ccagcgtcgt gagctcgcgc gtgggccatt tcttcgacgg
     3781 gcacggcacg ctcgagcgca tcgacgcgct cgacggcgag ccgcggtcga tgctgcgcat
     3841 caacgacgag gtgcgcacgc tgtggccgcg ctaccgcgtc gccgtgatcg agccggccga
     3901 cccgcgcgcg gccttcccgg cgctggtcag cggcaacgag aagcacatcc ccgacttcta
     3961 cgagctgcac gtgcagcccg cccagcgcat cgccggccac gacgccgacc gcctgctgct
     4021 gaagtcgcgc gacgacagcc gcttcgaccg cgtgatctgg gccgaccacg ccaccggcct
     4081 gctgctgcgc gtggacgtgc agctggcggg gcgcacgctg gaatcgtccg gcttctcgga
     4141 cgtcaacatc ggcgtcaagc ccgacgccgc ggccgtctac aacgaactgc accgggccga
     4201 cggcttccgc atcgtccggc cggcgatcga gcacacgaag ctggataccg agggctggtc
     4261 gatgcaggcg tcgctgctgc cgccgggctt catggcgctg gactgcgtgc ggcgcgcgct
     4321 ggacgtgccc ggcgacaccg ccggccaggc caaggtgctg caggcgatct ggtcggacgg
     4381 catcacgcac gtgtcgctgt tcatcgagcc gttccagccg caacgccacc gcagcgaggg
     4441 cctgacggtc atcggcgcca cgcacacgct cacgcgccgg gtcaacgaca tgtggctcac
     4501 ggccgtcggc gacgtcccgc ccacggcgct cgaccagttc gttcgcgcgt tggaacacaa
     4561 gtaagacctg gcaacgccgg gtgcaccggc cgcggtcaca ataccgaacc cctgttccgg
     4621 agccaccgct cgatgtccaa gcccaacgcc cccttcgccg cccgcgccac cctggcgttc
     4681 gccacggtcc tggccgccgc gttgccgacg ctgtcgcggg ccgaggccac gcccacgccg
     4741 gtgcccgtcc aggcgcccga gacggccgtg cgcggcctgc ccgacttcac cggcctcgtc
     4801 gagacggtgg gcccggcggt cgtcaacatc cgtaccaaca tcaagcccaa ggccgccgcc
     4861 ggcgaccagt cgctgcaggc gcagatgcag gagctgatcg aggaatacat ccgccgccac
     4921 ggcggccgcg gcggccgcaa cctgccgccc ggcggcaacg acgcgcccga agacgacgac
     4981 cagcccgagc agggctacat cggctcgggc ttcctgctgt cgcccgacgg ctacgtgatg
     5041 accaacgccc acgtcgtcga gggcgccgac gaggtcaccg tcacgctgac cgacaagcgc
     5101 gagttcaagg ccaggatcat cggcagcgac cagcgtaccg acgtcgcgct gctcaagatc
     5161 gactcgaccg gcctgccgtt cgtcaaggtc ggcagcgtcg accgcctgaa ggtcggcgag
     5221 tgggtgatcg ccatcggctc gccgttcggc ctggagagca ccgtcaccgc cggcatcgtc
     5281 agcgccaagc agcgcgagac gggcgactac ctgcagctgc tgcagaccga cgtcgcgatc
     5341 aatcccggca actcgggcgg cccgctgatc aacatgcgcg gcgaggtcgt gggcatcaac
     5401 tcgcagatcc tgagcccgtc gggcaccagc gcgggcatct cgctggccat ccccatcgac
     5461 gaggcgatcc gcgtgtccga ccagctgcgc gcgagcggcc acgtcacgcg cgggcgcatc
     5521 ggcgtgcaga tcggcatcgt caacaagcag gtggccgagg ccgtcggcct gggcaagccg
     5581 gtcggcgcga tggtgcagag cgtgcaggcc ggcctgccgg cggagaaggc cggcatcgag
     5641 gcgggcgaca tcatcaccaa ggtgcagggc aagccggtcg accgctattc cgaccttccg
     5701 cgcctggtgg gcatggtcaa gccgggcgag aaggtgcaac tgcaggtgtt ccacgacggc
     5761 aagtacaagg acgtcacggc cgacgtggtc gacttcgggc cggagacgcc gcccacggtc
     5821 gccgcgggcc ccgaccccga ggccatcgcc aacaccaagg ccacgctcgg cctgaaggtg
     5881 gt
//
LOCUS       DATCRO010000163         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_530558, whole genome shotgun sequence.
ACCESSION   DATCRO010000163 DATCRO010000000
VERSION     DATCRO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530558"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIN75_04475"
     CDS             <1..217
                     /locus_tag="VIN75_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY8973450.1"
                     /translation="ATTSSRHLAGQAQAMPDKDLLYRVPNQSSLDGNTVEMDVERIAF
                     ADNALRYESNLTVINAKIKSLLSAVQP"
     gene            248..670
                     /gene="flgC"
                     /locus_tag="VIN75_04480"
     CDS             248..670
                     /gene="flgC"
                     /locus_tag="VIN75_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY8973451.1"
                     /translation="MPFNSTSMNIFNVAGSAMTAQSQRMNVTASNMANAESVAGPDGM
                     PYRAKQVVFQVAPSAYGAGDIGGVKVAGVVEDPSPPKLVFDPKSPHANAEGYVAMPNV
                     NVVEEMTNMISASRSYQANVEVLNTAKTLMVKTLTLGQ"
     gene            718..1404
                     /locus_tag="VIN75_04485"
     CDS             718..1404
                     /locus_tag="VIN75_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEY8973452.1"
                     /translation="MAISDTSSITGQNAASAVGGNSSTSNVDSEQRFLKLLVTQLNNQ
                     DPLNPMQNAELTSQLAQMSTVSGIEKLNSTLSGLVNQTGSNQLLQATSLIGQNVLSPG
                     DVLATRAPEEGKDPAMQAFAVQLPGTASDVQIKILDASGKVVRTIDAGSMNEGVNAVT
                     WDGKDDVGAVVPAGAYRFTVEATNGGTSVKSTALTFSQVAAVKQGASGVTLELSSGAS
                     IGLSDVRLFL"
     gene            1472..>2244
                     /locus_tag="VIN75_04490"
     CDS             1472..>2244
                     /locus_tag="VIN75_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY8973453.1"
                     /translation="MAFSQGISGLGVAAANLDVIGNNIANSGTVGFKSAAATFQDVYA
                     GSRVGMGASVSGVTQNFSQGVTQSSSRPLDVAILNGDGFFRLTSATGEVMYSRNGQFT
                     RDKDGYIVNASGLRLTGFGVNANGGVSGGTPAAIQIPTTPMTPKATTSIDAEFNLDAR
                     KAAPTKTPFNPADSDTFNYSNAVGPIYDSLGNPHDVSVYFVKSATPANTWSMYGTIDG
                     AAIGAGALGTMTFDAAGKMTAPASGQFSLGTINLTNGAAA"
BASE COUNT          441 a          824 c          673 g          306 t
ORIGIN      
        1 ggccactacc tcgtcgcgcc acctcgcggg acaggcgcag gccatgcccg acaaggatct
       61 gctctaccgc gtgcccaacc agtccagcct cgacggcaac acggtcgaga tggacgtcga
      121 gcgcatcgcc ttcgccgaca acgcgctgcg ctacgagtcg aacctcacgg tcatcaacgc
      181 caagatcaag tcgctgctct ctgcggtgca gccctaagca gcgcagcaag cagcaggagt
      241 cccttccatg cccttcaaca gcacctccat gaacatcttc aacgtggccg gatcggccat
      301 gaccgcgcag tcgcagcgca tgaacgtgac cgcgagcaac atggccaacg ccgagagcgt
      361 ggccgggccc gacggcatgc cctaccgcgc gaagcaggtg gtgttccagg tcgcgccctc
      421 ggcgtacggc gcgggcgaca tcggcggcgt caaggtggcc ggcgtggtgg aagacccttc
      481 tccgccgaag ctcgtgttcg accccaagag cccgcatgcc aacgccgaag gctacgtggc
      541 gatgcccaac gtcaacgtgg tggaggagat gaccaacatg atctccgcat ctcgcagcta
      601 ccaggccaac gtggaagtgc tcaacaccgc caagacgctg atggtcaaga cgctgaccct
      661 cggccagtaa ttccccggca agacaagaac ccaagaaaga acaggcacga acgcgccatg
      721 gccatctccg atacctcgtc catcaccgga cagaacgccg cctccgcggt cggcggcaac
      781 agcagcacct cgaacgtcga cagcgagcag cgcttcctca agctgctggt cacgcagctg
      841 aacaaccagg acccgctcaa tccgatgcag aacgccgagc tcacctcgca gctcgcgcag
      901 atgagcacgg tcagcggcat cgagaagctc aacagcacgc tctcgggcct ggtgaaccag
      961 accggctcca accagctgct gcaggccact tcgctgatcg ggcagaacgt gctgtctccc
     1021 ggcgacgtcc tcgccaccag ggcgccggag gagggcaagg acccggccat gcaggccttc
     1081 gccgtgcagc tgccgggcac cgcctccgac gtgcagatca agatcctcga cgccagcggc
     1141 aaggtggtgc gcaccatcga cgcgggctcg atgaacgaag gcgtcaacgc cgtgacgtgg
     1201 gacggcaagg acgacgtggg cgccgtggtg cccgctggcg cctaccgctt cacggtcgag
     1261 gccaccaacg ggggcacctc cgtgaagtcg accgcgctca ccttctcgca ggtcgccgcc
     1321 gtcaagcagg gcgccagcgg cgtgacgctg gagctgtcgt cgggcgcgag catcgggctg
     1381 tcggacgtgc gcctgttcct ctgagcttct ttcccttccc caccgcattc atcgcaaacg
     1441 cgccgcatcc aacggcagac aaggaaccat catggctttt tcccagggca tcagcggact
     1501 cggcgtggcc gcggccaacc tcgacgtcat cggcaacaac atcgccaact cgggcaccgt
     1561 cggcttcaag tccgcggcgg ccaccttcca ggacgtgtac gcgggctcgc gcgtgggcat
     1621 gggcgcatcg gtgtcgggcg tgacgcagaa cttctcgcag ggcgtgaccc agtcgagcag
     1681 ccgcccgctg gacgtggcca tcctcaacgg cgacggcttc ttccgcctca ccagcgccac
     1741 cggtgaagtc atgtactcgc gcaacggcca gttcacgcgc gacaaggacg gctacatcgt
     1801 caacgcatcg ggcctgcgcc tcaccggctt cggcgtgaac gccaacggcg gcgtcagcgg
     1861 cggcacgccg gcggccatcc agatcccgac cacgccgatg acgcccaagg cgaccaccag
     1921 catcgacgcc gagttcaacc tcgacgcgcg caaggcggcg cccaccaaga cgccgttcaa
     1981 ccccgccgac tccgacacct tcaactactc gaacgccgtc ggccccatct acgactcgct
     2041 gggcaacccg cacgacgtgt ccgtgtactt cgtcaagagc gccacgcctg ccaacacctg
     2101 gagcatgtac ggcacgatcg acggcgccgc catcggcgcc ggcgcgctcg gcacgatgac
     2161 cttcgacgcc gccggcaaga tgaccgcgcc ggccagcggc cagttcagcc tcggcaccat
     2221 caacctgacc aacggcgccg ctgc
//
LOCUS       DATCRO010000164        12274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_2004, whole genome shotgun sequence.
ACCESSION   DATCRO010000164 DATCRO010000000
VERSION     DATCRO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VIN75_04495"
     CDS             <1..1989
                     /locus_tag="VIN75_04495"
                     /inference="COORDINATES: protein motif:HMM:NF040115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HEY8973454.1"
                     /translation="ALTTDQVAAIATADLRALGSVALDALTTTQLNALTTTQVAALTT
                     AQVSSLTTTNLDALTTAQFAALTTTQVAALTTDQVANLQTADLVALTTSQIRALGTDQ
                     VVALTTSEVAAMTTAQVNALTTTDIAALTTDQVAALSTADLRALNTVSLDALTTTQLN
                     ALATDHIAAFSTAQVAALTTDQVANLSTTSLDAFGTAQFAALTTTQVAALTTDQVANL
                     QTADLAALTTTQFHALSTDQVAALTTADFAAITTTQFHALATDAIAAMSTADVHALTT
                     AVVHSMTTDQVAALTTAEIQAFTTAHIAALTTDHIAALTTTEAAAFTSAEFAALSTAH
                     IAAFTTDQLAHLTTTEIGAFTTAQMHVLTTDQIAAFVTDTIQAFTTTDLHSLTSTQAN
                     AFSNDIPSMTDAQVNALITADPIVLDLTGAGIHTSNATTQGVNFDITGTGKVDHVGWT
                     TGSNEGFLVLDKNHDGQVNSGAEMFGTGMLMPDGTRAASGYQALSQYDTNHDGVINAN
                     DAVFKQLQVWVDANHDGKVEAGEMKSLHDLGIVSLDLHATAGNTADNGNSIGLTSSYT
                     TASGATHQMADVNLTKADTSSTTVDPSIVPAHAAATPHLSELLAAPSTDLLPGHVENA
                     ATGTAGGHATTAQVHGMLDQRLLEEEERQRLNNSNPLI"
     gene            2045..6610
                     /locus_tag="VIN75_04500"
     CDS             2045..6610
                     /locus_tag="VIN75_04500"
                     /inference="COORDINATES: protein motif:HMM:NF040115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HEY8973455.1"
                     /translation="MSFLSRLLGPVVGHMVDRWFHHGHSPSMGAPTTTVSGPLHSLEI
                     AALSSADVATLTAAQLRSIAPSDMVFFNTEALFGFSSVQLNVLSTSQFVDLLPAEIAA
                     LTPQQVGWVSTADLRAMHTAQAAALTSAQLAALTTTQVAALTTSDVAALSTAALPGLG
                     STQIQALTSTEFATLSTTQLDALAADQVAALTSAQVRQLTSAELNALGTEQLQAFTSA
                     QLAALSTAQVASLATAQVALLATGEIAALGTAQLQALTTAQVQALTVDELAALSTSSI
                     AGLTTAAIRSLGTAQLDSLSSDQLGALSTTQVAALSTLQVQGLGIAGLAALTTTEIGA
                     LTTRQVASLTTDQVQSLDTADFHAFSSRQVAALTTTQVGALGTDLIAGLGSAGIAALS
                     TVDIRALTTTQVAALTTDELHGLTTAQVAALTSAQALVLTPGELSSLSTASVRALATA
                     VVAALPAAELDALATATLAALATAQVAALTTAQVSSIAAQTLDALTSSQILALTTRQV
                     GALTTDQLPALDTDVIAAFSSTQLRALSTDQFAALTTDEIAAIAPASIPALTTLQVSG
                     LATQTLDALSTAQFAALTTSQVASLTTIQVASLSTDDIAALTTAQAAALTTTEAGVLS
                     DAQLAALSTTDLAALRTAALDALATAQFAGLSDVQLAALTTRQVAGLGSAQLAALDSD
                     QIAALTTAQFAALSTAALSSLATDQVAALATIDVQALTSAQLRGLTTAQFDALTTDAL
                     AALSTAAVAAITTAEIANLQPDHLAALSPEQFSALTTRQIAAMTSDQIAGFSLAQIAA
                     LPPADIAALSTSAILGLGTAGIQVLGTADIAAMRTLQVAALSTDEVAALSQPQLAALS
                     TSAIRALGTAALEALGVAGFTSLTTRQLAALSSVQVQHLATDELAALTSTQLNAFTTA
                     QIAALTTTEVQALTADQLAGLASQDLRAFGSAAIRALDTTGLMALTSTEIGQLTTAQV
                     AAFTTSQVGALSVDQLAMLTTSDIVALGTAGVQALGSLQLDALSMAQVAALTTSEIHA
                     LTTTQVTGLDDAHLAAFTTRQFGSLTTDVIAALSTDQVVALASADFGVLTTAQVAALS
                     PDAISVLTVGEVAAMTSTQVSALNSLQAAALSTDDLAAFSSSALHALNTSAITGLTTD
                     QIGAMTTGEFHAFGTAQIAAFTSAQVLAFSTDVLDGLTTSQFAALTSTQFAVLTTDQF
                     THLASVDIAALTTAQAHALSTDNIVALSTDLLPALSTTDIAAMTTDQVSSFTPDQISH
                     MTDAQIGALFMATPVVLDLDGNGISTTSAAHGVKFDVTGTGTNAKYGWTSTTDGLLAI
                     DLNHDGKIDDGSELFGVGTKLANGRHAGNGYEAMAQFDSNGDGVLDAKDAHFKDLVVW
                     VDANHDGVSEAGEVKSLSQLGIVSLDLHGLAGHATDHGNLLGLTSSYTTADGATHAMA
                     DVWFAKDGTASTSASAAAASSHESTSTPSLGDLLAAPATELLPGGEPAHVARVHTADH
                     ASTALAHGLLDARRLDDDEAGRHAPLI"
     gene            6661..7329
                     /locus_tag="VIN75_04505"
     CDS             6661..7329
                     /locus_tag="VIN75_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HEY8973456.1"
                     /translation="MNQDSTPGILARAPFARITRMARRAGLALLCIAAPANVHAGPAL
                     RDGDVIFQTSRSPQSLAVQRATGSKYSHMGLVVFRDGRPTVLEAAATVRYTPLAEWIA
                     RGVDGHYVVKRLRDADTRLTPDATRRLLREGDRFVGRPYDLVFGWSDERIYCSELVWK
                     AFDRGMGLRVGRIQKIRDFNLSDPAVRAKLRERYGDHIPLDEPVISPVAMFESPILVT
                     VVDR"
     gene            7485..8612
                     /locus_tag="VIN75_04510"
     CDS             7485..8612
                     /locus_tag="VIN75_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973457.1"
                     /translation="MKMKNVLCLAAGGLATLMGATSAMAAGWTLTDLGTLGGTTSEAS
                     GLNDAGQVVGYAESADGTPYPVVWTNGVATQLSTRPGRAIAISPNGVIAGNVISATST
                     KIDATLLTGGNAIDQGTFGGSFSLVSTVNDNGQVGGYARTPDEKDHAFLGTASGMVDL
                     GTFGGVYSYVYGLNRSGLAVGSAYDASGMARCFRTTGGGLVDLGTFGGRFCNAFAVND
                     AGQITGGAASPAGVSHAFVFDSRGLVDLGTLGGTSSVGYAINRLGQVTGSSRTASNQT
                     HAFFHDGTRMIDLGSLGGLVTTGRAVNAAGQVIGTSTLPNSSTSQPFFYANGVMVNLN
                     TAVPGLSNIDVSSIRLNEAGQIAGTGMIGGNKHAFLLTPAP"
     gene            8945..10294
                     /locus_tag="VIN75_04515"
     CDS             8945..10294
                     /locus_tag="VIN75_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973458.1"
                     /translation="MLNRDVNARFEDWLLSPSEGLDFEAKRWLDMSDKECQGLVAKAL
                     IALENHGGGFLVVGYAEDDQKRLRPDPARPPSLEQFSADAINAIIKRCAEPAFHVEVF
                     IQRHPETGDEYPLLRATGRSKVPVRSCSATPNKSLQQNVYYIRRPGPNSDGPKTASEW
                     DQLMRRCVMNQREDIIAVLRAFSSFGASSGEALPPTKTELLDSFAEASKLAWFKLNDS
                     LDVADAARISKGYYCFSARVLGISRALDAAAILSTLEGARKYTGWPSFVVLHQPETRP
                     KLVGGAVQAWTAKCPYPGVSYADYWRITPSGEFFLLRGYDEDSLESKIKAAPVPGTAF
                     EPTLPVWRLADFLLRVVEVAEAMFEPDFEVVVECEWTGLKGRRTVSLNHRIFFNSVTC
                     SEDVVKTNGTFTQAMLQDILPEVVRRLTSPLYLHFEFTKFESRFYEGEIEQMKSGKF"
     gene            complement(10882..>12274)
                     /locus_tag="VIN75_04520"
     CDS             complement(10882..>12274)
                     /locus_tag="VIN75_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8973459.1"
                     /translation="DVTVHYNGNDMGPVTAAEIADFRRRHGIARPYFMTSGSRADYKN
                     ASLFFNAFARFGDARKDYAIVCTGGGTLDDASRAAAGEAGVHVAIFDEHDLRCAYAGA
                     LALVYPSRYEGFGLPVLEAMACECPAITSTASSLPEVGGDAVLYIELGDTEEDQTFEL
                     LQRVQRPEVRADLVERGRAQARKFSWKTMADGVAATLQAAASAHPAPAPRVMPAAVRA
                     DDQLLPIGALRCRLPAASSLAGLKREHRMYDRLASSLGAVLDAGDGAVIAGAGFGTSL
                     VALAGAQPALSLLAIERDAARFAYLRSNATGLPSASVQLLQAELGSPQLAGVDAVLRE
                     AGLDGVRLLVSTIGAAGADVLAGASDLCRHVSPMLYFTCQIGSDANGADAWRERLRAL
                     WAFGYTGFWLFDNFGNPVCEVTQPRALDQMLDYLSRQNQRRASRTLYYYDVLAFTDRD
                     AARAARAVDLHTR"
BASE COUNT         2033 a         4459 c         4052 g         1730 t
ORIGIN      
        1 gcgctgacca ccgaccaggt ggccgcgatc gccaccgccg acctgcgcgc cctcggcagc
       61 gtcgcgctcg acgccctgac gaccacgcag ctgaacgcgc tgacgaccac ccaggtggcc
      121 gcgctgacga cggcgcaggt ctcgtcgctg accacgacca acctggacgc gctgaccacg
      181 gcccagttcg ccgcgctgac gacgacgcag gtggccgccc tgacgaccga ccaggtcgcc
      241 aacctgcaga cggcggacct cgtcgcgctg acgacgagcc agatccgcgc gctgggcacc
      301 gaccaggtcg tcgcgctgac gaccagcgag gtcgccgcga tgacgacggc ccaggtcaac
      361 gcgctgacga cgaccgacat cgccgcgctg accaccgacc aggtggccgc gctgtcgacc
      421 gccgacctgc gcgcgctcaa caccgtctcg ctcgacgcgc tgacgacgac gcagctgaac
      481 gcgctggcca ccgatcacat cgccgcgttc tcgaccgccc aggtggcggc cctgacgacc
      541 gaccaggtcg ccaacctgtc gacgaccagc ctggacgcct tcggcaccgc gcagttcgcg
      601 gcactgacga cgacgcaggt ggccgcgctg acgaccgacc aggtcgccaa cctgcagacc
      661 gccgatctcg ccgcgctgac gacgacgcag ttccacgcgt tgagcaccga ccaggtcgcc
      721 gcgctgacga cggccgactt cgccgccatc acgacgacgc agttccacgc cttggccacc
      781 gacgccatcg ccgcgatgtc gacggcggac gtccacgcgc tgacgaccgc ggtcgtgcac
      841 tcgatgacca ccgaccaggt ggccgcgctc accacggccg agatccaggc cttcacgacg
      901 gcgcacatcg ccgccctgac gacggatcac atcgccgcgc tgacgacgac ggaggccgcc
      961 gccttcacga gcgccgagtt cgccgccctg agcacggccc acatcgccgc gttcacgacc
     1021 gaccagctcg cgcacctgac gaccacggag atcggcgcgt tcacgacggc gcagatgcac
     1081 gtcctcacga ccgaccagat cgcggcgttc gtgaccgaca ccatccaggc cttcacgacg
     1141 accgacctgc attcgctcac gagcacgcag gccaacgcgt tcagcaacga cattccgtcg
     1201 atgacggacg cgcaggtcaa cgcgctgatc acggccgacc cgatcgtgct cgacctgacg
     1261 ggcgccggca tccacacctc caacgcgacc acccagggcg tcaacttcga catcacgggc
     1321 acgggcaagg tggaccacgt gggctggacg accggttcga acgagggctt cctggtcctc
     1381 gacaagaacc acgacggcca ggtcaacagc ggcgccgaga tgttcggcac cggcatgctg
     1441 atgcccgacg gcacgcgcgc ggcaagcggc taccaggccc tgtcgcagta cgacaccaac
     1501 cacgacggcg tgatcaacgc caacgacgcg gtgttcaagc agctgcaggt gtgggtcgac
     1561 gcgaaccacg acggcaaggt cgaggccggc gagatgaagt cgctgcacga cctgggcatc
     1621 gtctcgctcg acctgcatgc cacggcgggc aacaccgcgg acaacggcaa ctcgatcggg
     1681 ctgacgtcgt cgtacacgac cgccagcggc gccacgcacc agatggcgga cgtcaacctg
     1741 accaaggccg acacgtcgtc cacgaccgtc gacccgagca tcgtgccggc gcatgcggcc
     1801 gcgacgccgc acctgagcga gctgctggcg gcgccgtcga ccgacctgct gccgggccac
     1861 gtcgagaacg cagccaccgg caccgcgggc ggccacgcca ccacggcgca ggtgcacggc
     1921 atgctcgacc agcgcctgct cgaggaagag gaacgccagc gcctgaacaa cagcaacccg
     1981 ttgatctgac ggacggcccg gccggccccg cgcaacccta cgggccggcc agccacccac
     2041 cgccatgagc tttctctcgc ggctgctggg acccgtggtc ggccacatgg tcgaccgctg
     2101 gttccatcac ggccattcgc cgagcatggg cgccccgacg accaccgtct cggggccgct
     2161 gcactcgctc gagatcgccg ccctgagctc ggccgacgtg gccacgctca cggcggcgca
     2221 gctgcgctcg atcgcgccgt cggacatggt gttcttcaac accgaggcgc tgttcgggtt
     2281 ctcgagcgtg cagctgaacg tgttgtcgac gtcgcagttc gtcgacctgc tgcctgcgga
     2341 gatcgctgcg ctgacgccgc agcaggtcgg atgggtgtcg accgccgacc tgcgcgcgat
     2401 gcacacggcg caggccgcgg cgctgaccag cgcgcagctg gccgcgctga ccacgacgca
     2461 ggtggccgcg ctgacgacct ccgatgtcgc ggcgctgtcg accgccgccc tcccgggcct
     2521 cggttcgacg cagatccagg cgctcacgag cacggagttc gcgacgctgt cgacgaccca
     2581 gctcgacgcc ctcgccgccg accaggtggc tgcgctgacc agcgcgcagg tgcgccagct
     2641 cacgagcgcc gagctcaacg cgctcggcac ggagcagctg caggccttca cgagcgcgca
     2701 gctggccgcg ctgtccaccg cgcaggtcgc gtcgctggcc accgcgcagg tcgcgctgct
     2761 ggccaccggc gagatcgccg cgctcggcac ggcgcagctg caggcgctga cgacggcgca
     2821 ggtgcaggcg ctgacggtcg atgagctcgc ggcgctgtcg acgtcgtcca tcgccggcct
     2881 gaccacggcg gcgatccgca gcctgggcac ggcgcagctc gattcgctgt cttccgacca
     2941 gctcggcgcc ctgtcgacca cgcaggtcgc cgcgttgtcc acgctgcagg tgcagggcct
     3001 gggcatcgcc ggcctcgcgg cgctgaccac cacggagatc ggcgcgctca ccacgcgcca
     3061 ggtcgcgtcg ctgaccaccg accaggtgca gtcgctggac acggccgact tccacgcgtt
     3121 cagctcgcgc caggtggcgg cgctgacgac gacgcaggtc ggcgcgctgg gcaccgacct
     3181 gatcgcgggg ctgggcagcg ccggcatcgc ggcgctgtcg accgtcgaca tccgcgcgct
     3241 gaccaccacg caggtcgcgg cactgaccac cgacgagttg cacgggctga cgaccgcgca
     3301 ggtcgccgcg ctcacgtccg cgcaggcgct ggtgctgacg ccgggcgagc tctcgagcct
     3361 gtcgacggcc agcgtgcgcg cgctggcgac cgccgtcgtc gccgcgctgc cggccgccga
     3421 gctcgacgcg ctggccaccg cgacgctggc ggcgctggcg acggcgcagg tcgcggcgct
     3481 gacgacggcg caggtgtcgt cgatcgccgc gcagacgctc gatgcgctga cctcgtcgca
     3541 gatcctcgcg ctgaccacgc ggcaggtcgg cgcgctgacc accgaccagc tccccgcgct
     3601 ggacaccgac gtgatcgccg cgttctccag cacgcagctg cgcgcgctgt cgaccgacca
     3661 gttcgccgcg ctgaccaccg acgagatcgc cgcgatcgcg cccgcgagca tcccggcgct
     3721 gaccacgctg caggtgtcgg gcctggcgac gcagacgctc gacgcgttgt cgacggcgca
     3781 gttcgcggcg ctgacgacct cgcaggtcgc ctcgctgacc acgatccagg tggccagcct
     3841 gtcgaccgac gacatcgccg cgctcacgac cgcgcaggcc gcggcgctga ccaccaccga
     3901 ggccggcgtg ttgagcgacg cgcagctcgc cgcgctgtcg acgaccgacc tcgccgcgct
     3961 gcgcaccgcg gcgctcgacg cgctggccac cgcgcagttc gccggcctca gcgacgtgca
     4021 gctcgccgcg ctcaccacgc gccaggtcgc cggactgggc agcgcgcagc tggcggcact
     4081 cgactcggac cagatcgccg cgttgaccac ggcgcagttc gccgcactgt cgacggcggc
     4141 gctgtcgtcg ctggccaccg accaggtcgc ggcgctcgcc acgatcgacg tgcaggcgct
     4201 gacgagcgcc cagctgcgcg gcctgaccac cgcgcagttc gacgcgctga ccaccgacgc
     4261 gctggccgcg ctgtcgacgg ccgcggtggc cgcgatcacg accgcggaga tcgccaacct
     4321 ccagcccgac cacctcgcgg cgctgtcgcc ggagcagttc tcggcgctga ccacgcgcca
     4381 gatcgcggcg atgacgtccg accagatcgc gggcttctcg ctggcgcaga tcgccgccct
     4441 gcccccggcc gacatcgcgg cgctgtccac gagcgccatc ctgggcctgg gcaccgccgg
     4501 catccaggtg ctgggcacgg cggacatcgc ggccatgcgc accctgcagg tcgcggcgct
     4561 gtcgaccgac gaggtggccg cgctgtcgca gccgcagctc gcggcgctgt ccacgagcgc
     4621 gatccgcgcg ctgggcacgg cggcgctcga ggcgctcggc gtcgccggct tcacgtcgct
     4681 gacgacgcgg cagctcgccg cgctgagctc ggtccaggtg cagcacctgg cgaccgacga
     4741 actcgcggcg ctcacgtcga cccagctgaa cgcattcacg acggcgcaga tcgcggccct
     4801 gaccaccacc gaggtgcagg cgctgacggc cgaccagctg gccggcctcg cgtcgcagga
     4861 cctgcgcgcc ttcggctccg cggcgatccg cgccctcgac accaccggcc tgatggcgct
     4921 cacgtcgacc gagatcggcc agctgacgac ggcacaggtc gccgcgttca cgacgagcca
     4981 ggtcggcgcg ctctcggtcg accagctggc gatgctgacg acctccgaca tcgtcgccct
     5041 gggcaccgcg ggtgtgcagg cgctgggctc gctgcagctg gacgcgctgt cgatggcgca
     5101 ggtcgcggcg ctcaccacga gcgagatcca cgcgttgacg acgacccagg tcacgggcct
     5161 ggacgacgcc cacctggccg ccttcacgac gcgccagttc ggctcgctga cgaccgacgt
     5221 gatcgccgcg ctcagcaccg accaggtcgt cgcgctcgcc tcggcggact tcggcgtgct
     5281 gaccaccgcg caggtggcgg cgctgtcgcc ggacgcgatc tcggtgctga cggtgggcga
     5341 ggtcgcggcg atgacgtcga cgcaggtctc cgcgctgaac agcctgcagg ccgcggcgct
     5401 gtcgacggac gacctggccg cgttcagcag ctcggcgctg catgccctga acacgtcggc
     5461 gatcacgggc ctgacgaccg accagatcgg cgcgatgacg accggcgaat tccacgcctt
     5521 cggcaccgcg cagatcgcgg ccttcacgtc cgcgcaggtg ctggcgttca gcacggacgt
     5581 cctcgacggc ctgacgacct cgcagttcgc cgcgctgacg tcgacccagt tcgcggtgct
     5641 caccaccgac cagttcacgc acctggcctc ggtcgacatc gccgcgctga cgaccgcgca
     5701 ggcccatgcg ctgagcacgg acaacatcgt cgcgctgtcg accgacctgc tgcccgcgct
     5761 gtccaccacg gacatcgccg cgatgaccac cgaccaggtc tcgtcgttca cgccggacca
     5821 gatctcgcac atgaccgatg cccagatcgg cgcgctgttc atggcgacgc cggtggtgct
     5881 ggacctggac ggcaacggca tcagcaccac ctccgcggcg cacggcgtga agttcgacgt
     5941 cacgggcacc ggcaccaacg cgaagtacgg ctggacatcg acgaccgacg gcctgctcgc
     6001 gatcgacctg aaccacgacg ggaagatcga cgacggcagc gagctgttcg gcgtcggcac
     6061 gaagctggcg aacggccggc acgcgggcaa cggctacgag gcgatggcgc agttcgacag
     6121 caacggggac ggcgtgctcg atgcgaagga cgcgcacttc aaggacctgg tcgtgtgggt
     6181 cgatgcgaac cacgacggcg tcagcgaggc cggcgaagtc aagtcgctgt cgcagctggg
     6241 catcgtgtcg ctggacctgc acggactggc ggggcacgcg acggatcacg gcaacctgtt
     6301 ggggctgacg tcctcgtaca ccaccgcgga cggcgccacg catgcgatgg cggacgtctg
     6361 gttcgccaag gatgggacgg cctcgacgag cgcgtcggcg gcggccgcct cgtcgcacga
     6421 atcgacatcg acgccgtcgc tgggcgacct gctggcggcg ccggcaacgg aattgctgcc
     6481 gggtggcgag ccggcgcatg tcgcgcgcgt tcacaccgcc gaccacgcgt cgacggcctt
     6541 ggcgcacgga ctgctggacg cgcggcggct cgacgacgat gaggccgggc ggcatgcgcc
     6601 gttgatctga ggcccggcct cgttcgtgcg ccggctcccg attcgggtag gcttcgcgag
     6661 atgaaccagg acagcactcc gggcatcctc gcgcgggccc ccttcgcgcg gatcacgcgc
     6721 atggccaggc gggcgggcct cgccttgctg tgcatcgcgg ctccggcgaa cgtgcacgcc
     6781 ggcccggcgc tccgtgacgg cgacgtcatc ttccagacgt cgcgatcccc gcagtcgctg
     6841 gcggtgcaac gcgccacggg gtcgaaatac agccacatgg ggctcgtcgt gttccgcgac
     6901 ggccggccca ccgtgctcga agcggccgcc acggtccggt acaccccgct cgcggagtgg
     6961 atcgcacgcg gcgtcgacgg ccattacgtg gtcaagcgcc tgcgcgacgc cgacacccgc
     7021 ctgacgcccg acgcgacgcg ccgcctgctg cgcgagggag atcgcttcgt cgggcgcccg
     7081 tacgacctcg tgttcggctg gtccgacgag cggatctact gctcggagct cgtctggaag
     7141 gccttcgatc gcggcatggg actgcgcgtc ggacggatcc agaagatccg cgacttcaac
     7201 ctgtccgatc ccgcggtgcg ggcgaagctg cgcgaacgct atggcgatca catcccgctg
     7261 gacgagcccg tcatctcgcc ggtggcgatg ttcgagtcgc cgatcctggt cacggtcgtg
     7321 gaccgctgaa acaggtccga tctcgcgtcc gctgcaatcg ctcgtcaggt ccgattcttc
     7381 ctggctcact ccttgagcca cggctccgcg acactggatt caccccgatg cccggcgact
     7441 tcgcgccggc atcggctcag tgcaatcctt caatgggagt cgtcatgaag atgaagaacg
     7501 ttctgtgcct ggccgccggc ggcctcgcga ccctgatggg cgccacctcc gcgatggcgg
     7561 cgggctggac cctgaccgac ctgggtaccc tggggggcac caccagcgag gccagcggcc
     7621 tcaacgacgc gggccaggtg gtgggatatg cggaaagcgc cgacgggacg ccgtacccgg
     7681 tggtctggac caacggcgtg gccacgcagc tcagcacgcg ccccgggcgg gcgatcgcga
     7741 tcagcccgaa cggcgtcatc gcaggcaacg tgatcagcgc gacctcgacg aagatcgacg
     7801 ccaccctgct gacgggcggc aacgccatcg accagggcac cttcggcggc agcttcagcc
     7861 tcgtctcgac ggtcaatgac aacggccagg tgggcggcta tgcgcgaacg cccgacgaga
     7921 aggatcatgc cttcctcggc acggcgtccg gcatggtgga cctcggcacg ttcggcggcg
     7981 tctacagcta tgtctacggc ctgaaccggt ccggcctggc cgtgggcagc gcctacgacg
     8041 cctcgggcat ggcgcgctgc ttccgcacca ccggcggcgg cctcgtcgac ctggggacgt
     8101 tcggcggccg cttctgcaac gccttcgccg tcaacgacgc gggccagatc accggcggcg
     8161 ccgcctcccc cgccggcgtc agccacgcct tcgtgttcga cagccgcggc ctggtcgacc
     8221 tcggcacgct cggcggcacc agcagcgtcg gctacgcgat caaccgcctc ggccaggtca
     8281 ccggctcgtc gcgcacggcc tccaaccaga cccacgcctt cttccacgac ggcacccgca
     8341 tgatcgatct cggctcgctg ggcggcctcg tcaccaccgg ccgcgccgtc aacgccgcag
     8401 gccaggtgat cggcacctcg acgctgccca acagcagcac cagccagccc ttcttctacg
     8461 cgaacggcgt gatggtcaac ctgaacacgg ccgtgccggg cttgtcgaac atcgatgtga
     8521 gcagcattcg cctgaacgaa gcgggccaga tcgcgggaac cggcatgatc ggcgggaaca
     8581 agcacgcgtt cctgctcacg ccggcgcctt gaggcgcgag ggcgccaagc cgccactgcg
     8641 ggcgcggcgg cgcttccatt cgtcgatcgc gacaaggagc gccggcaaac aggctcgcgg
     8701 ccgacgcatt ggcgcggcgc cgcgcccgag tcgagcgacg ggccaaggtg cctgcgttcc
     8761 ctctggtgag tgaagatgag cggcgccttt agacccagga acagtcgctc ggcatatcgc
     8821 ggtagaaggt ccgctgcggc tgcgaagccg taatgcagac cagtgccgag ggcgctatag
     8881 ggcacactct gacgaagatc tggctggtcg gcgcattggc cagaatcagc atgcaggagg
     8941 aacgatgtta aaccgcgacg tcaacgcacg gtttgaagac tggcttctca gtccgagtga
     9001 gggattggat tttgaagcaa agcgatggtt ggacatgagc gacaaggaat gccaaggcct
     9061 tgtcgcgaag gctctgatcg cgttggaaaa tcatgggggc ggatttttgg tagtcggata
     9121 cgccgaggat gaccagaaac gattgcggcc cgatccagcc cgtccaccgt cactcgagca
     9181 atttagtgcc gacgcaatca acgcgatcat taagcggtgc gccgagccgg cctttcatgt
     9241 tgaggtgttc attcaaaggc acccagagac cggtgacgaa tacccactcc tgcgcgcgac
     9301 cggacgctcg aaagtcccgg ttcgatcttg cagtgctacg ccaaacaaaa gcctgcaaca
     9361 gaacgtctac tacattcggc gccctggccc gaacagcgac gggccaaaga ccgcctccga
     9421 atgggatcaa ctgatgcgtc gttgcgttat gaatcaacgt gaggacatca tcgccgtcct
     9481 aagagcgttt tcgtctttcg gagcctcgag cggcgaggcc ttgccaccaa cgaaaactga
     9541 acttctcgat tcgtttgcgg aggcatcgaa gctcgcatgg ttcaagttga acgactcact
     9601 cgatgttgcc gatgctgcac gcatctccaa aggatactac tgcttctccg cacgcgttct
     9661 cggcatatca agagcgcttg atgcggcagc catcctatcg acgctcgaag gggctcgaaa
     9721 atacaccgga tggccatcgt ttgtcgtcct gcatcagccc gaaacacgtc cgaagcttgt
     9781 gggaggtgct gtgcaagcat ggacggccaa gtgcccctat cctggcgtga gctatgcaga
     9841 ttattggcgc ataactccca gcggggaatt tttcttgctg cgcggctatg acgaggattc
     9901 attggaatca aagatcaagg cggcccctgt acccgggacg gcttttgagc caacgctccc
     9961 agtttggcgc ttggcagatt tcctacttcg agtggtagag gtcgcggaag caatgttcga
    10021 accggacttt gaggtcgtcg tcgagtgcga gtggactggg ctcaagggtc gccgcacagt
    10081 gtctctaaac caccgcatct tttttaacag cgtcacgtgc tcagaagatg tggtcaaaac
    10141 aaatggcaca ttcactcaag cgatgctgca ggacattctt ccggaggtcg tgcgcaggct
    10201 cacctcaccg ctctaccttc atttcgaatt cacgaaattc gagtcgcggt tttatgaggg
    10261 cgagatcgaa cagatgaagt cagggaagtt ttgatttcac tgattgcagc agttgcggtc
    10321 ggcgctgcgg cgatagcagt cgccacgccg caacgcttcc gtcgagcgcc gcaagccagt
    10381 ttccaggcca atgggcatcc tcaaccggac atcacagcct tagcggtgga cgcctcttga
    10441 cttggtcgcc gcgcggcgca gatccgaacc cgcgacgccg gaggcgtcgc gctcgtcgga
    10501 cacgagaagg aacgccggcg gcatggcccg tcgttcgcgt caagcccggc tccctgccga
    10561 ggcgatggcc gcgggcatcg acgcggcctc gatggcccgt cgatgccaag cccctccaag
    10621 agaatttgac gtccttcccc cgcgaatgtt gtctgcaggc ctgccatggt cggcacggct
    10681 cgccggggga gtctttttcg cgaggttaag gaggaggagg ccgaaggccg acgggggaca
    10741 cgagcgaaaa agactccccc ggcgagtcgt gccctcgcga gaaacccacc gcacgccgca
    10801 cgccgcacgc cgcacgccgc acgccccacg cctcacgccg atcaacgcaa gatgcaagac
    10861 gcaagaccca agcgctccaa ctcaccgcgt atgcagatca acagcccgcg ccgcccgcgc
    10921 cgcatcccga tccgtgaacg ccagaacatc gtagtagtac agcgtccgcg acgcccgccg
    10981 ctggttctgc cgcgacaggt agtcgagcat ctggtccagc gcccgcggct gcgtcacctc
    11041 gcacaccgga ttgccgaagt tgtcgaacag ccagaagccc gtgtacccga aggcccacaa
    11101 ggcccgcagc cgctcacgcc aggcatccgc cccgttcgca tcgctgccga tctggcaggt
    11161 gaagtacagc atcggcgaga cgtgccggca caggtcggac gcgccggcca ggacgtccgc
    11221 ccccgccgca ccgattgtcg agacgagcag ccgcacgcca tcgagccccg cctcgcgcag
    11281 caccgcatcc acgcccgcca gctgcggcga cccgagctcg gcctgcaaca gttgcacgct
    11341 cgccgacggc aaccccgtcg cgttgctgcg caggtaggcg aagcgcgcgg cgtcgcgttc
    11401 gatcgccagc aggctcagcg ccggctgcgc gccggcgagc gcgaccagcg atgtgccgaa
    11461 acccgcgccc gcgatcaccg cgccgtcgcc cgcatccagc accgcgccca acgacgacgc
    11521 gaggcggtcg tacatccggt gctcgcgctt gaggcccgcc agcgagctgg cggccggcag
    11581 gcggcagcgc agcgcgccga tcggcagcag ctggtcgtcc gcgcgcacgg ccgcgggcat
    11641 cacgcgcggc gcgggcgcgg ggtgtgcact ggcggcggcc tgcagcgtgg cggcgacgcc
    11701 atcggccatc gtcttccagc tgaacttgcg cgcctgcgcg cggccgcgct cgaccaggtc
    11761 ggcgcgcacc tcggggcgct gcacgcgctg cagcagctcg aaggtctggt cctcctcggt
    11821 gtcgccgagc tcgatgtaga gcaccgcgtc gccgccgacc tccggcagcg acgaggccgt
    11881 cgacgtgatc gccgggcact cgcaggccat cgcctcgagc accggcaggc cgaagccctc
    11941 gtaccgggac gggtacacga gcgccagcgc gccggcatag gcgcagcgca ggtcgtgctc
    12001 gtcgaagatc gccacgtgca cgccggcctc gcccgcggcg gcgcggctcg cgtcgtccag
    12061 cgtgccgccg ccggtgcaca cgatcgcgta gtccttgcgg gcgtcgccga agcgcgcgaa
    12121 ggcgttgaag aacagcgacg cgttcttgta gtcggcgcgg ctgcccgagg tcatgaagta
    12181 cgggcgcgcg atgccgtggc ggcggcggaa atcggcgatc tccgcggccg tgaccggacc
    12241 catgtcgttg ccgttgtagt gcacggtgac gtcc
//
LOCUS       DATCRO010000165         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_890725, whole genome shotgun sequence.
ACCESSION   DATCRO010000165 DATCRO010000000
VERSION     DATCRO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890725"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VIN75_04525"
     CDS             complement(<1..937)
                     /locus_tag="VIN75_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973460.1"
                     /translation="MNQATFNFAPRGKRADIAAARRPARVQASQMRGDEAADPGDVLG
                     FDYARFGMVPPAPCLWPGHPIREGWERGRRLMTRRTRPATLAVTRWLALRLAAWQRGE
                     AFDDHQITPQVLRSLEVAAHCPITGRALGNDAVVVPVDVQRGWAAGNLMLVSPAIADR
                     WTAIDWAIAKDALARAEADPQNDVDGLPLRHWRRIVALKSLATPLPHDEAARIPLLVL
                     PPNRLRLVNPIQELQAVLTLQLSVPGWGQRARTVADSLPQALRTEFNLFFNSLLAQAL
                     RQGHGDAKPEMRDALAAAWGGEVVMRRWGRFAALVD"
     gene            1024..2052
                     /locus_tag="VIN75_04530"
     CDS             1024..2052
                     /locus_tag="VIN75_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8973461.1"
                     /translation="MDRTERFYRIELLIKRQAGVTFDQLLDELEVSPATLKRDLQYLR
                     DRMDAPIVYDRADNVYRFEAGAAEAANGGPLARERGGKTHELPGVWFSEKELHALLTM
                     HQLIAGLDDGGVLGRHLQPLLDKLHGMLGASGDEAQALMRRVRIVNPAKRAVASRWFE
                     EAVSAVLSRRRLLISYYTRSKRSESERVLSPQRLLHYRNTWYLDAWCHASEGLRRFAL
                     DAVRAAKVLDEPARDLPLPEVEDALDQGYGIFGGARLRQATLHFSSEAAQWVAQEEWH
                     PQQQLKPQSDGSLRMRIPYSEPTELVMDVLRHGPNVRVVSPPELAQLVRDQLRETLAA
                     YEDAASDA"
     gene            2049..2612
                     /gene="greB"
                     /locus_tag="VIN75_04535"
     CDS             2049..2612
                     /gene="greB"
                     /locus_tag="VIN75_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY8973462.1"
                     /translation="MNKAFTKETDSDEDDDGPSLPALPAGGKNYITPQGHDRLRAELL
                     HLIDEERPKVVETVHWAASNGDRSENGDYIYGKKRLREIDRRIRFLIKRLDIAVVADP
                     SAHHGKDQVFFGATVTYANDAGDERTVTIKGIDEVDSLQGEVSWVSPIARTLLKAREG
                     DTLQLVTPAGVETIEVLAVSYPAPAAH"
     gene            2636..3436
                     /locus_tag="VIN75_04540"
     CDS             2636..3436
                     /locus_tag="VIN75_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY8973463.1"
                     /translation="MKVSSLIAALLAAASVAGAPRAHAAPVAAEKPKAIPVYGVQVVR
                     TTPHDINAFTEGLFFLNGYFYESTGLDGHSSVRKVKVETGQVLQRANLPPEMFGEGIA
                     PWHGNLVGLTWKSQIGYVLDLDSFDTKGKFGYPGEGWGLTHDDTELVMSDGTPDIRFL
                     NPNTLIETHRIHVTAQGQPVDQLNELEWVEGEIYANIWQTDRIARIDPKTGEVVGWID
                     CKGLLPMKDFIPNHTDVLNGIAYDPATRRLWVTGKFWPKVFEIKLVRR"
     gene            3576..>4520
                     /locus_tag="VIN75_04545"
     CDS             3576..>4520
                     /locus_tag="VIN75_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY8973464.1"
                     /translation="MSPTAPSSDQPFSRDDTKFLGHPLGLGWLAFCEFWERFSYYGMQ
                     GLLGLYLTKYLFLPEHITQVMGIDAGRRAIEFITGPMTPFQFGSLVVGLYGGLVYFTP
                     VFGGIIADRWLGRTRAVVLGASLMALGHFLMAFESALLPALACLLLGVGCFKGNIAAQ
                     VGDLYGPDDKRRASAFQIFMLAVQIAVIAAPIVCGTLGERVGWHWGFGSAGVGMLIGL
                     FVYLAGRRHLPPEPQRGAAKPEAPPIGRRGAMQVALLVAMIPLLMLSIVGNQQFNNAY
                     PLWSQQHMDMMVGGFEVPVTWLQAVDATVSFVTMLGAIA"
BASE COUNT          704 a         1610 c         1542 g          664 t
ORIGIN      
        1 cgtccaccag tgccgcgaag cgaccccagc ggcgcatcac cacctcgccc ccccaggccg
       61 cggcgagcgc gtcgcgcatc tcgggcttgg cgtcgccgtg gccctggcgc aacgcctggg
      121 ccagcagcga gttgaagaac aggttgaact cggtgcgcaa agcctgcggc agcgagtcgg
      181 cgaccgtgcg ggcgcgctgg ccccagcccg ggacggacag ctgcagcgtc agcacggcct
      241 gcagctcctg gatcgggttg accaggcgca ggcggttggg cggcagcacc agcagcggga
      301 tgcgcgctgc ctcgtcgtgc ggcagcggcg tggcgagcga cttcagcgcc acgatgcggc
      361 gccagtggcg cagcggcagg ccgtcgacgt cgttctgcgg atcggcctcg gcgcgcgcca
      421 gcgcgtcctt ggcgatggcc cagtcgatgg ccgtccagcg atcggcgatc gccgggctca
      481 ccagcatcag gttgcccgcg gcccagccgc gctgcacgtc caccggcacc acgacggcgt
      541 cgttgcccaa cgcacggccc gtgatcgggc agtgcgccgc gacctccagc gaacgcagca
      601 cctgcggcgt gatctggtgg tcgtcgaagg cctcgccgcg ctgccaggcc gccaggcgca
      661 gcgccagcca gcgcgtgacg gccagcgtgg ccggacgcgt gcgacgcgtc atcagccggc
      721 ggccgcgctc ccagccctcg cgaatcgggt ggccgggcca caggcagggc gctggcggca
      781 ccatgccgaa gcgggcgtag tcgaagccga ggacgtcgcc cgggtcggcg gcctcgtcgc
      841 cgcgcatctg ggaggcctgg acgcgcgcgg ggcggcgtgc ggcggcgatg tcggcgcgct
      901 tgccgcgggg ggcgaagttg aaggtggcct ggttcatcgt cgtgtccttc ggtctgatct
      961 tggagtgagg ccagtttcgg ccgtcacaag ctcacacgat gagctacggc aaaatggggt
     1021 cccatggaca ggacagaacg cttctaccgg atcgagctgc tgatcaagcg gcaggccggc
     1081 gtcaccttcg accagctcct cgacgagctc gaggtctccc cggcaacgct caagcgcgac
     1141 ctgcagtacc tgcgcgatcg catggacgcg cccatcgtct acgaccgggc cgacaacgtg
     1201 taccgcttcg aggcgggcgc ggccgaggcc gccaacggcg gtccgctcgc acgcgagcgc
     1261 ggcggcaaga cgcacgagct gcccggcgtg tggttctcgg aaaaggaact tcacgcgctc
     1321 ctgacgatgc accagctgat cgccggcctg gacgatggcg gcgtgctcgg gcgccacctg
     1381 cagccgctgc tggacaagct gcacggcatg ctgggcgcct cgggcgacga ggcgcaggcg
     1441 ctgatgcggc gcgtgcgcat cgtcaacccc gccaagcgcg cggtggccag ccgctggttc
     1501 gaggaggccg tcagcgcggt gctgagccgg cggcgcctgc tgatcagcta ctacacgcgc
     1561 agcaagcgca gcgagtccga gcgcgtgctg tcgccacagc gccttctgca ctaccgcaac
     1621 acctggtacc tggacgcctg gtgccacgcc agcgaaggcc tgcgccgctt cgcgctcgac
     1681 gcggtgcgcg cggccaaggt gctggacgag ccggcacgcg acctgccgtt gcccgaggtg
     1741 gaagacgccc tcgaccaggg ctacggcatc ttcggcggcg cccgcctgcg ccaggccacg
     1801 ctgcacttca gctccgaggc ggcgcagtgg gtagcgcagg aggaatggca tccgcagcag
     1861 caactcaagc cccagtcgga cggctcgctg cgcatgcgaa tcccgtactc cgagcccacc
     1921 gagctggtga tggacgtgct gcgccatggc ccgaacgtgc gcgtggtgtc gccgccggaa
     1981 cttgcgcagc tggtccggga tcaactccgc gagaccctgg cggcctacga ggacgccgcg
     2041 agcgacgcat gaacaaggcc ttcaccaagg aaaccgacag cgacgaggac gacgacggcc
     2101 cgtcgctgcc cgcgctgccg gccggcggga agaactacat caccccgcag ggccacgacc
     2161 gcctgcgcgc cgaactgctg cacctgatcg acgaggagcg ccccaaggtc gtcgagacgg
     2221 tgcactgggc cgcctccaac ggcgaccgca gcgagaacgg cgactacatc tatggcaaga
     2281 agcgcctgcg cgagatcgac cgccgcatcc gcttcctgat caagcggctg gacatcgccg
     2341 tcgtggccga cccgtcggcc caccacggca aggaccaggt cttcttcggc gccaccgtca
     2401 cctacgccaa cgacgcgggc gacgagcgca ccgtcaccat caagggcatc gacgaggtcg
     2461 attcccttca gggcgaggtc agctgggtgt cgccgatcgc gcgcacgctg ctcaaggcgc
     2521 gcgagggcga cacgctgcag ctggtgacgc cggcgggcgt cgagacgatc gaggtgctgg
     2581 ccgtgagcta ccccgcgccc gccgcgcatt gacgaacatt tccggatacg agcgaatgaa
     2641 ggtttcctcc ctgatcgcgg cgctgctggc cgccgcgagc gtggccggcg cgccgcgcgc
     2701 ccacgcggcg cccgtcgccg ccgagaagcc caaggccatc ccggtctacg gcgtccaggt
     2761 ggtgcgcacg accccgcacg acatcaacgc gttcaccgaa ggcctgttct tcctgaacgg
     2821 ctacttctac gagagcacgg gcctggacgg ccactcgtcg gtgcgcaagg tcaaggtcga
     2881 gacgggccag gtcctgcagc gcgcgaacct gccgcccgag atgttcggcg agggcatcgc
     2941 gccctggcac ggcaacctcg tcgggctcac ctggaaatcg cagatcggct acgtgctcga
     3001 cctggacagc ttcgacacca agggcaagtt cggctacccc ggcgaaggct ggggcctgac
     3061 gcacgacgac accgagctgg tgatgagcga cggcacgccc gacatccgct tcctgaatcc
     3121 gaacacgctg atcgagacgc accgcatcca cgtcaccgcg cagggccagc cggtcgacca
     3181 gctcaacgag ctcgaatggg tggaaggcga gatctacgcc aacatctggc agaccgaccg
     3241 catcgcgcgc atcgacccga agaccggcga ggtcgtcggc tggatcgact gcaagggcct
     3301 gctgccgatg aaggacttca tcccgaacca caccgacgtg ctcaacggca tcgcctacga
     3361 cccggccacc aggcggctgt gggtgacggg caagttctgg cccaaggtct tcgagatcaa
     3421 gctcgtcagg cgctgattcc ccgcgacttt tcgtcggttt ccggcctctc gcaccgccga
     3481 atcctccgtt catccgcggc tgcgggcttt ccgccttgcg ccgggcggat ggctgcacca
     3541 cagtcccgca tcgtttttcc ccggatttcc tgatcgtgtc cccgaccgca ccctcttccg
     3601 atcaaccgtt ctcgcgcgac gacaccaagt tcctcggcca cccgctgggc ctgggctggc
     3661 tggccttctg cgagttctgg gaacgcttct cgtactacgg catgcagggc ctgctgggcc
     3721 tgtacctgac caagtacctg ttcctgcccg agcacatcac gcaggtgatg ggcatcgacg
     3781 ccggccgccg tgccatcgag ttcatcaccg ggccgatgac gccgttccag ttcggctcgc
     3841 tcgtggtcgg cctgtacggc ggcctcgtct atttcacgcc ggtgttcggc ggcatcatcg
     3901 ccgaccgctg gctgggtcgc acgcgagccg tcgtgctggg cgccagcctg atggccctgg
     3961 gccacttcct gatggcgttc gaaagcgcgt tgctgccggc cctggcctgc ctgctgctgg
     4021 gcgtgggctg cttcaagggc aacatcgccg cccaggtggg cgacctgtac ggtccggacg
     4081 acaagcgccg cgccagcgcc ttccagatct tcatgctggc ggtgcagatc gccgtcatcg
     4141 ccgcgcccat cgtctgcggc acgctgggcg agcgcgtggg ctggcactgg ggcttcggct
     4201 cggccggcgt gggcatgctg atcggcctgt tcgtctacct ggccgggcgc cgccacctgc
     4261 cgccggagcc gcagcgcggt gcggccaagc ccgaagcgcc gcccatcggc cgtcgcggcg
     4321 cgatgcaggt ggccctgctg gtggccatga tcccgctgct gatgttgtcc atcgtcggca
     4381 accagcagtt caacaacgcc tacccgctgt ggtcgcagca gcacatggac atgatggtgg
     4441 gcggcttcga ggtgccggtg acctggctgc aggccgtcga cgccacggtg agcttcgtca
     4501 ccatgctggg cgccatcgcc
//
LOCUS       DATCRO010000166         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_46867, whole genome shotgun sequence.
ACCESSION   DATCRO010000166 DATCRO010000000
VERSION     DATCRO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46867"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIN75_04550"
     CDS             complement(<1..471)
                     /locus_tag="VIN75_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8973465.1"
                     /translation="MSLRVLLVEDDRELCRTLKDAMTAEGYDIFTAASLSEGFALLTG
                     LSTRANGAPVDLVLLDLGLPDGDGEQLLAALRNKHALPVIVFSARESDGQKVRLLDAG
                     ADDYLVKPFSIRELLARMRVGLRHRGTSVTPAVTRYSVGALNIDLGTREVTLAGQ"
     gene            complement(530..2110)
                     /locus_tag="VIN75_04555"
     CDS             complement(530..2110)
                     /locus_tag="VIN75_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973466.1"
                     /translation="MTDDDPNSPHRSARWPWAWACVVWAVAWLLMLGLDRHIGLAQAA
                     ITLVLASALAAIWLPLVSSLITTTAAVLAFDWFIVPPKHSFSVELSEDVLLVIEILFL
                     SWVVTALLARGRVLAARARRNARHADQMRRLGDTLRDAEDPLRRGEALREALGELLGQ
                     PARLLLLRETGTEPADDAATTTLGEPGADELVGLWHCVRLCRAFGPGTGHEPELPALY
                     LPLRGTETQAYGAALVPLGLDADITEDVLAQAQALCDQLGQLLHRARTQRDAESANAL
                     AQAQAMRNALLAAISHDYRTPLAAILGAASSLRDQGAKLAPEQRERLAHSIMAETRQL
                     SRLTDNTLQLVRLDAPGVKLNLDWESPDELAGAAIERARRHDPERRLKVWAEPGLPLV
                     RCDAVLFSQMLENLIDNALKYSDAPAPVELHVRRHGAHIVFAVRDSGPGVEPAWRERI
                     FDVFQRGDWRTGTHNSAAQRGAGVGLAVCRAIARAHGGELQLRPRSQGGSSFECWLRE
                     EEAPRVEERVPAADGGPA"
     gene            complement(2215..3042)
                     /locus_tag="VIN75_04560"
     CDS             complement(2215..3042)
                     /locus_tag="VIN75_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8973467.1"
                     /translation="MTTIHEYTVGERLLTLDNVCVHYDRPILDGVTGHIDNIRRAGRQ
                     QGQVVCLLGPSGIGKSQLFRCIAGLQRPTSGSVRLNGSQREVQAGEVGVVAQNYPLFN
                     HRTVWGNLMLAALRHGRSVDAARETCLALLDKFRLADHARAHPMMLSGGQRQRVAIAQ
                     QLLCSSHFLLMDEPFSGLDPLVKTAVCETLIDVSTSHELNTIIVVTHDIECAIRISDT
                     LWLLGRQLDASGRPVGGASIRQVYDLASMGLAWDPDIESRPEFFELAAELKARFATL"
     gene            complement(3039..3830)
                     /locus_tag="VIN75_04565"
     CDS             complement(3039..3830)
                     /locus_tag="VIN75_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973468.1"
                     /translation="MKPVNLLLPFTPVRVRWLHVLVAAQAAAALGLWAASGSRTLPSP
                     LEVGRAWTDLVAHQGLLVELWVSVKVSLLALALSTAAAVALACLATAPLFMPLARLAA
                     ALRFLGFAGLTYLFMLMSGDAIALRVAILAFGMFVFMVTGLLSDLAATSRDPIDHCRT
                     LGMRHWRITAEVVLLGKADVILDLVRQNAAVGWTLLTLVEGLTRSAGGIGAMLLNENR
                     YFLLAGVFAIQATILAVGLLQDALLSMLKETACPWSTLGKETATS"
     gene            complement(3889..>4162)
                     /locus_tag="VIN75_04570"
     CDS             complement(3889..>4162)
                     /locus_tag="VIN75_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8973469.1"
                     /translation="QMLDQVSVSGLVVQVSGHTDNVGDPSSNLALSRRRAEAVSAWLQ
                     ANASSSFPKERVRTRAYGDGQPVASNATADGRARNRRVDIALVRTE"
BASE COUNT          586 a         1461 c         1559 g          556 t
ORIGIN      
        1 ctggccggcg agggtcacct cgcgcgtgcc caggtcgatg ttgagcgcgc cgacgctgta
       61 gcgcgtgacg gccggcgtca cgctggtgcc gcggtggcgc aggccgacgc gcatgcgtgc
      121 gagcagctcg cggatgctga agggcttgac gaggtagtcg tcggcgccgg cgtcgagcag
      181 gcgcaccttc tggccgtcgc tctcgcgggc cgagaacacg atgacgggca acgcatgctt
      241 gttgcgcagc gccgcgagca gctgttcccc gtcgccgtcg ggcaggccca ggtccagcag
      301 cacgaggtcg accggggcgc cgttggcgcg cgtgctcagt cccgtgagca gcgcgaagcc
      361 ctcgctgagg ctggcggcgg tgaagatgtc gtagccctcg gccgtcatcg cgtccttgag
      421 cgtccggcac agttcacggt cgtcctcgac cagcagcacg cgcaggctca tcaggcaacc
      481 tccggcctgc gaaccccggt gcgggtcctt tcgggcggcc ggacggcgct caagccgggc
      541 ctccgtcggc cgccggcacg cgctcttcga cgcgcggggc ctcctcctcg cgcagccagc
      601 actcgaagct ggagccgccc tggctgcgcg ggcgcagctg cagctcgccg ccatgcgccc
      661 gggcgatcgc gcggcacacc gcgaggccga cgcccgcgcc gcgctgcgcg gcgctgttgt
      721 gggtgccggt gcgccagtcg ccgcgctgga agacgtcgaa gatccgctcg cgccaggccg
      781 gctccacgcc gggcccgctg tcgcgcacgg cgaagacgat gtgcgcgccg tgccggcgca
      841 cgtgcagctc caccggcgcc ggtgcgtcgc tgtacttcag ggcgttgtcg atcaggtttt
      901 cgagcatctg cgagaacagg acggcgtcgc aacgtaccag cggcaggccg ggttcggccc
      961 acaccttcag gcggcgctcc ggatcgtgcc ggcgcgcgcg ctcgatcgcc gcgccggcga
     1021 gctcgtcggg cgactcccag tcgaggttga gcttgacgcc aggcgcatcg aggcgcacga
     1081 gttgcagcgt attgtcggtc aggcggctga gctggcgcgt ctcggccatg atgctgtgcg
     1141 ccagccgttc gcgctgctcg ggggcgagct tcgcgccctg gtccctcagg gacgaggccg
     1201 cgcccaggat cgcggccagc ggcgtgcggt agtcgtgcga gatcgcggcc agcagcgcgt
     1261 tgcgcatcgc ctgcgcctgc gccagcgcgt tggccgactc cgcgtcgcgc tgcgtgcggg
     1321 cgcggtgcag cagctggccc agctggtcgc acagggcctg cgcctgcgcg aggacgtcct
     1381 ccgtgatgtc cgcgtcgagt cccagcggca cgagcgccgc gccataggcc tgtgtctcgg
     1441 tgccgcgcag cggcaggtac agcgcgggca gctcgggttc gtggccggtg cccgggccga
     1501 atgcgcggca caggcgcacg caatgccaca ggccgacgag ctcgtcggcg ccgggctcgc
     1561 ccagcgtcgt cgtggcggcg tcgtccgcgg gctcggtgcc ggtctcgcgc agcagcagca
     1621 ggcgtgcggg ctggcccagc agttcgccga gcgcctcgcg cagggcctcg cctcgccgca
     1681 gcggatcctc ggcgtcgcgc agcgtgtcgc cgaggcggcg catctggtcg gcgtggcggg
     1741 cgttgcgccg ggcgcgcgcg gcgagcacgc gtccgcgcgc gagcagcgcg gtcacgaccc
     1801 agctcaggaa caggatctcg atgaccagca gcacgtcctc ggacagctcg acgctgaacg
     1861 agtgcttggg cggcacgatg aaccagtcga acgccagcac cgccgccgtg gtcgtgatca
     1921 gcgaggacac cagcggcagc cagatggccg ccagcgccga cgccagcacc agcgtgatgg
     1981 cagcctgcgc gaggccgatg tggcggtcca gccccagcat cagcagccag gcgacggccc
     2041 agacgacgca ggcccaggcc cacggccagc gggcgctgcg gtgcggggag ttcgggtcgt
     2101 cgtcggtcat cggcgcaacc gtaccagagg tcgcggcctc gcggacggcg cgccaggcgc
     2161 gtcgcaggtg cgcgggccag aagtcggccg aggcgtcgat gccgggcagc gggatcacag
     2221 cgtcgcgaag cgggccttga gctccgccgc cagttcgaag aattccggcc gcgactcgat
     2281 gtcggggtcc cacgcgaggc ccatgctcgc gaggtcgtag acctgccgga tcgaagcgcc
     2341 gcccaccggc cggccgctcg cgtcgagctg gcgcccgagc agccacagcg tgtcggagat
     2401 gcggatcgcg cactcgatgt cgtgcgtgac gacgatgatc gtgttgagtt cgtgcgaggt
     2461 cgacacgtcg atgagcgtct cgcacactgc ggtcttcacg agtggatcga ggcccgagaa
     2521 cggctcgtcc atcagcagga aatgcgagct gcacagcagc tgctgggcga tcgcgacgcg
     2581 ctggcgctgg ccgcccgaca gcatcatcgg atgcgcgcgg gcatggtcgg ccaggcggaa
     2641 cttgtccagc agcgcgaggc aggtctcgcg cgcggcatcc acgctgcggc cgtgccgcag
     2701 cgccgccagc atcaggttgc cccagacggt gcggtggttg aacagcggat agttctgcgc
     2761 gacgacgccc acctcgcccg cctgcacctc gcgctgcgag ccgttcaggc gcacgctgcc
     2821 ggacgtcggc cgctgcaggc cggcgatgca gcggaacagc tgcgacttgc cgatgcccga
     2881 cggcccgagc aggcacacca cctggccctg ctggcggccc gcgcggcgga tgttgtcgat
     2941 gtgtccggtg acgccatcga ggatggggcg gtcgtagtgc acgcagacat tgtccagggt
     3001 cagcaggcgt tcgccgacgg tgtattcgtg gatcgtggtc atgacgttgc cgtctccttt
     3061 ccgagcgtgc tccaggggca cgcggtttcc ttgagcatcg acagcagcgc gtcctgcagc
     3121 aggccgaccg cgaggatcgt ggcctggatc gcgaacacgc cggcgagcag gaagtaacgg
     3181 ttctcgttga gcagcatcgc gccgatgccg ccggccgagc gcgtcaggcc ctcgaccagc
     3241 gtcagcagcg tccagccgac ggccgcgttc tggcggacga ggtcgaggat cacgtcggcc
     3301 ttgcccagca gcacgacttc ggccgtgatg cgccagtggc gcatgcccag cgtgcggcag
     3361 tggtcgatcg ggtcgcgcga ggtcgcggcg aggtccgaca agaggccggt caccatgaac
     3421 acgaacatgc cgaacgcgag gatcgccacg cgcagcgcga tcgcgtcgcc ggacatcagc
     3481 atgaacaggt acgtcaggcc ggcgaagccg aggaagcgca gcgcggcggc cagccgcgcg
     3541 agcggcatga acagcggcgc cgtcgccagg caggcgagcg cgaccgccgc ggccgtggac
     3601 agcgcgagcg cgagcaggct caccttcacg ctgacccaca gttcgacgag caggccctgg
     3661 tgcgcgacga ggtcggtcca cgcgcggccc acctccagcg gcgagggcag cgtgcgcgag
     3721 ccgctcgccg cccagaggcc gagcgcggcc gcggcctgcg ccgcgaccag gacatgcagc
     3781 cagcgcacgc gcacgggcgt gaacggcagc agcagattga cgggtttcaa ggtcttctcc
     3841 gtggcgagcc gccccgggcc agggcacggg gcggctcgct gctggcgctc actcggtgcg
     3901 caccagcgcg atgtcgacgc ggcggttgcg ggcgcggccg tcggccgtgg cgttgctggc
     3961 caccggctgc ccgtcgccgt aggcgcgcgt gcgcacccgc tccttgggga acgacgacga
     4021 ggcgttggcc tgcagccagg cgctcacggc ctcggcgcgg cggcgcgaca gcgcgaggtt
     4081 ggacgacggg tcgccgacgt tgtccgtgtg gcccgagacc tgcacgacca ggcccgacac
     4141 cgacacctgg tcgagcatct gg
//
LOCUS       DATCRO010000167         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_609473, whole genome shotgun sequence.
ACCESSION   DATCRO010000167 DATCRO010000000
VERSION     DATCRO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609473"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1300)
                     /locus_tag="VIN75_04575"
     CDS             complement(68..1300)
                     /locus_tag="VIN75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY8973470.1"
                     /translation="MALQPSTHPRVHSSHATSHAAHASHAAHAASKAKPALTSEEGEG
                     GGFAAQLLKAQPAAGDKDLKAADSRVEAARKPDGDQDGKSADAKDASDGQPDKDGKPT
                     DPKAVLDPAALLAAQQAQQQVQPQAIAPATTSLADKSAGDHDEFATDPLGALRKAAGK
                     AGKDDGRQALLANLAGGGTAAAPATAAGLSAKGAAANNGDIAAALAKAEGAVKAATEA
                     RDALAAMLPQPDPMAVQAGTVGAPANAPLDPNQGLQAQPPALPAQATLPMPPHSPAFA
                     PALGHQIDVWMKGGVQHAEVQLSPQDLGPIRVKIEMDGAHARVQMSADVQSTRDALQQ
                     AMPQLSEQLGQVGLSLSGGGVSDQPAYQQAQAHAQGAGNGRQAAGQPSGASHGDDLAV
                     AASARRPIQPRGLLDTYA"
     gene            complement(1340..1792)
                     /gene="fliJ"
                     /locus_tag="VIN75_04580"
     CDS             complement(1340..1792)
                     /gene="fliJ"
                     /locus_tag="VIN75_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY8973471.1"
                     /translation="MKRLALLKTLLEREQKRRDEQMANVRNAVANAQAQQQQADGLSG
                     YRTEYCRKWSAQFQQAATIEILRSYQGFLSRLDQAITQQQSVLEHAHNMVEAHRQRLV
                     QREIRVATVERLIQRREAMLAKAADRRDQKNLDELAQRLATLRAHAGL"
     gene            complement(1857..>2650)
                     /locus_tag="VIN75_04585"
     CDS             complement(1857..>2650)
                     /locus_tag="VIN75_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEY8973472.1"
                     /translation="GMMARYTQADVIVVGLIGERGREVKEFIEDILGDEGLARSVVVA
                     APADAPPLVRMQGANYATAVAEHFRDRGQHVLLLMDSLTRFAMAQREIALAIGEPPAT
                     RGYPPSCFAKLPQLVERSGNAGAGGGSITAFYTVLTEGDDPQDPIADAARGILDGHIV
                     LSRELAESGHFPAIDVEKSISRVMTSVAPREHLAAARVARQALSKVTKARDLIQLGAY
                     TPGADPELDAAVKMSPKLTALLQQDMHERATMAESRHQLTKAISL"
BASE COUNT          283 a          907 c         1032 g          428 t
ORIGIN      
        1 gaactgcagg attcaagacc tgacctcgca ccgcgccgcg acccgcgctg ccgccgcgcc
       61 gggcgcgtca ggcgtaggtg tcgagcagtc cgcgcggctg gatcggccgc cgggcggacg
      121 cggcgacggc gagatcgtcg ccatggctgg cgcccgacgg ctggccggcg gcctggcggc
      181 cgttgccggc gccctgggcg tgggcctggg cctgctggta ggccggctgg tcggacacgc
      241 cgccgcccga caggctcagg ccgacctggc ccagttgctc ggacagctgc ggcatcgcct
      301 gctgcagggc gtcgcgggtg gactgcacgt ccgcgctcat ctggacgcgg gcgtgggcgc
      361 cgtccatctc gatcttgacg cggatcgggc ccagatcctg cgggctcagc tggacttcgg
      421 cgtgctggac gccgcccttc atccagacat cgatctggtg gcccagcgcc ggcgcgaagg
      481 cggggctgtg cgggggcatg ggcagtgtcg cctgggccgg cagcgcgggc ggctgggcct
      541 gcaggccctg gttggggtcg agcggggcgt tggccggcgc gccgaccgtg ccggcctgca
      601 cggccatcgg gtcgggctgc ggcagcatgg cggccagcgc gtcgcgcgcc tcggtggcgg
      661 ccttgacggc gccctcggcc ttggccagcg cggcggcgat gtcgccattg ttggcggccg
      721 cgcccttggc cgacaggccg gcggccgtcg cgggcgccgc cgccgtcccg ccgccggcca
      781 ggttggccag cagcgcctgg cggccgtcgt ccttgccggc cttgcccgcg gccttgcgca
      841 gcgcgcccag cgggtcggtg gcgaattcgt cgtggtcgcc ggcggacttg tcggccagcg
      901 aggtggtggc cggggcgatc gcctggggct ggacctgctg ctgggcctgc tgggcggcca
      961 gcagcgcggc cggatcgagc acggccttgg ggtcggtggg cttgccgtcc ttgtccggct
     1021 ggccgtccga cgcgtccttg gcgtcggcgg acttgccgtc ctggtcgcca tcgggcttcc
     1081 tggcggcctc gaccctggaa tcggcggcct tcaggtcctt gtcgccggcc gccggctgcg
     1141 ccttgagcag ctgcgccgcg aagccgccgc cctcgccttc ctcggaggtc agcgcgggct
     1201 tggccttgct ggcggcatgc gccgcgtgcg aggcatgggc ggcgtgcgac gtggcgtgcg
     1261 aactgtggac gcgggggtgg gtgctgggtt gcagggccat ggtgtcgctc gaaaggatcg
     1321 gtgagtgttc gaaaatggtt cagaggccgg cgtgcgcgcg caaggtcgcg aggcgctggg
     1381 ccagctcgtc gaggttcttc tggtcgcgcc ggtcggccgc cttggccagc atcgcctcgc
     1441 ggcgctggat caggcgctcg acggtggcca cgcggatctc gcgctggacc agacgctggc
     1501 ggtgcgcctc gaccatgttg tgcgcgtgct cgagcaccga ctgctgctgc gtgatggcct
     1561 ggtccaggcg cgacaggaag ccctggtagc tgcgcaggat ctcgatggtc gcggcctgct
     1621 ggaactgggc cgaccatttg cggcagtact cggtgcggta gccggacagg ccgtcggcct
     1681 gctgctgctg cgcctgggcg ttggcgacgg cgttgcgcac gttggccatc tgctcgtcgc
     1741 ggcgcttctg ttcgcgctcg agcagggtct tgagcagggc caggcgcttc atgcggacgc
     1801 cttggcaggg gtggatcctg cgactgcgcg caggatgacg gcgatgtcgg cgttcgtcac
     1861 agcgagatcg ccttggtcag ctggtgcctg ctctcggcca tcgtcgcgcg ctcgtgcatg
     1921 tcctgctgca gcagcgcggt cagcttcggg ctcatcttga ccgcggcgtc gagctccgga
     1981 tcggcgcccg gcgtgtacgc gcccagctgg atcaggtcgc gcgccttggt caccttggac
     2041 agcgcctggc gcgcgacgcg cgcggcggcc aggtgctcgc gcggggcgac gctggtcatc
     2101 acgcgcgaga tcgacttctc gacgtcgatc gccgggaagt ggcccgactc ggccagctcg
     2161 cgcgacagca cgatgtggcc gtccaggatg ccgcgggcgg cgtcggcgat cggatcctgc
     2221 ggatcgtcac cctcggtcag tacggtgtag aaggcggtga tcgagccgcc gcccgcgccg
     2281 gcgttgcccg agcgctcgac cagctgcggc agcttggcaa agcagctggg cggatagccc
     2341 cgggtggccg gcggctcgcc gatcgccagc gcgatctcgc gctgcgccat cgcgaaacgg
     2401 gtcagcgagt ccatcagcag cagcacgtgc tggccgcggt cgcggaagtg ctcggccacg
     2461 gcggtggcgt aattggcgcc ctgcatgcgc accagcggcg gcgcgtcggc cggcgcggcc
     2521 acgaccaccg agcgcgcgag gccttcgtcg ccgaggatgt cctcgatgaa ttccttgact
     2581 tcgcggccac gctcgccgat cagcccgacg acgatcacgt cggcctgcgt gtagcgcgcc
     2641 atcatgccca
//
LOCUS       DATCRO010000168         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_237993, whole genome shotgun sequence.
ACCESSION   DATCRO010000168 DATCRO010000000
VERSION     DATCRO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237993"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VIN75_04590"
     CDS             <1..512
                     /locus_tag="VIN75_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973473.1"
                     /translation="GTYLMINHAWRHLVGEDRQYGPVGRFACWAVTFLGVVFAWVLFR
                     APDIHSALRIYAGMLGLNGLSLPVSMRSVAEGWHLPVALYFNDLFQGVPLPEQYSFGK
                     LMSTMALAGAIAFFAPAVAQMYEIGRGLTVDWQRRLFTRRVALGFGVMLTVAIFSISK
                     VSPFLYFQF"
     gene            520..1611
                     /locus_tag="VIN75_04595"
     CDS             520..1611
                     /locus_tag="VIN75_04595"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973474.1"
                     /translation="MSTTTSIPRPEAGTDTRAAATFLRWVAVTFLLIAGINIGLSMRF
                     GPIRGDLARVGGFAERDYEPGVPQASARIAPNTVDLADADVVVLGDSFSNKLLWQGEL
                     EALTGQRTLTFQYGQVGCISNWLRWLHGMKLKPGAQVIIESSERSFVARFNKMAECPK
                     ITPVPMHRRLETGEKSWFDTGLSLDIVYQGRVLLNSLRLDKQDVYRAGETVNVALRRD
                     DRFTDRRPDRLLYHVDDEGKNDWTPSQLANALAGLSAEKAAFAASGQRFSMLVMPDKS
                     SVYRDDIVAPRIRPSSVTRDLQAAGLFGMDTMTCFRTLATSMPDFYLPDDTHVGPNAF
                     RLLAAALAHGQQCPAPAAPASAAASAGLN"
     gene            complement(1604..2503)
                     /locus_tag="VIN75_04600"
     CDS             complement(1604..2503)
                     /locus_tag="VIN75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF022760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8973475.1"
                     /translation="MSSPCAPSNPASPDASTPGGATVRHFIITRFNLRGIEDTPSSAK
                     MVDSAYLAHRLELFERYCLPTVRSQTVQDFKWLVLFADETPAAIRSRVEAYAADWSNL
                     VPIWLPRGTSTVGPLVVGPYLDDSPQTLLTTRLDNDDGLARDYVAKVQRHARSTERLV
                     LQFPMGYVLHKDRVYLDRQEHNAFTTLVEPLPQGKSAGFNTIYKGSHSDIARLGRIVD
                     VDDEPSWLQVIHDSNVENYVRGSRQRIDCLQQRFAVDMPAAARPESGLEIGLDRIRTF
                     ATSTATRAARSIKYRLGLYDNVN"
     gene            complement(2503..>4436)
                     /locus_tag="VIN75_04605"
     CDS             complement(2503..>4436)
                     /locus_tag="VIN75_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973476.1"
                     /translation="TDGASCSTATCSADLATNTAVTLTAVPAANFTFGGWGGSCGGTS
                     TTCTVTLSTSKTVTANFSPKVPVAGAPWVRYVDTVTAPTTGGENSLGGYLSIFGSNFG
                     SASGLGTTTKVFIGGSEVANYRYLGAAKVGSQLGLQQLTVQVGNLGGLAVGTSAPVLV
                     VVNGVPSNMDATFTPTSGKVWFVSPTGNDSTAVAGDITHPLRYMQNMTTSTGAYFKMG
                     AGDQVVLRGGTWNDTNGVDTTWMRFGAGSAARNGTAKAWIHITAYPGPIKGNAIEDVH
                     YTTPSGTSGGIAGPWSAIAGTSGEYIAVSNLRMDVAGGAARDAAPINFQYTAGHWRVV
                     NNELGPWVAGNSAVLNAAGISGHGDTMLIYGNHIHDIAGLSDLQNHGIYADTTAQNWE
                     VAYNWINNMTGGSAIQFNDNEGGAGSYALPHGGTWAGFTGIKIHHNWLQNAAKYGVNF
                     NDQQSSKAGTYSGMIWDNVIIGTALPPLRINSTQPVQQLWFAYNTMYDCMTTTSGTGN
                     GYVRAEGWSAQSGVNNLFYDNIFAFGPDTVAGTQWFVDAGGTAASTTTYSFKNNLYSA
                     GTQLPNLFSTIGDLLGLSGDPLFASVSLTSPNFTTSSTSPARKSAKQALPSGFTVTDD
                     FAGTWRGAGTSDVGAFFTP"
BASE COUNT          629 a         1457 c         1602 g          748 t
ORIGIN      
        1 acggcaccta cctgatgatc aaccacgcct ggcgccacct cgtcggcgag gaccgccagt
       61 acggcccggt cgggcgtttc gcgtgctggg cggtcacgtt cctgggcgtc gtgttcgcct
      121 gggtgctgtt ccgcgcgccc gacatccaca gcgcgctgcg catctacgcg gggatgctgg
      181 ggctgaacgg gctgtcgctg ccggtgtcga tgcgctcggt cgcggagggc tggcacctgc
      241 cggtcgcgct gtatttcaac gacctgttcc agggcgtgcc gttgcccgag cagtactcgt
      301 tcggcaagct gatgtcgacg atggcgctgg ccggcgccat cgccttcttc gcgccggcgg
      361 tcgcgcagat gtacgagatc ggccgcgggc tgacggtcga ctggcagcgc cgcctgttca
      421 cgcgccgcgt cgcgctgggc ttcggcgtga tgctgacggt ggcgatcttc agcatcagca
      481 aggtcagccc gttcctgtac ttccaattct gaggccgccg tgtccacgac cacctccatc
      541 ccccgtccgg aagccggcac cgacaccagg gcggcggcca cgttcctgcg ctgggtcgcg
      601 gtgaccttct tgctgatcgc cggcatcaac atcggcctgt cgatgcgctt cggcccgatc
      661 cgcggcgacc tggcgcgcgt cggcggcttt gccgagcgcg actacgagcc gggtgtgccg
      721 caggcctccg cgcgcatcgc gcccaacacc gtggacctgg ccgatgccga cgtcgtcgtc
      781 ctgggcgact cgttcagcaa caagctgctg tggcagggcg agctcgaggc gctgaccggc
      841 cagcggacgc tgaccttcca gtatggccag gtaggctgca tctccaactg gctgcgctgg
      901 ctgcacggca tgaagctcaa gccgggggcg caggtcatca tcgagagctc ggagcgcagc
      961 ttcgtcgcgc gcttcaacaa gatggccgag tgcccgaaga tcacgccggt gcccatgcac
     1021 cgccgcctcg agacgggcga gaaatcgtgg ttcgacacgg gcctgagcct ggacatcgtc
     1081 taccagggcc gcgtgctgct gaactcgctg cgcctggaca agcaggacgt ctaccgcgcc
     1141 ggcgagacgg tcaacgtggc gctcaggcgc gacgaccgct tcaccgaccg ccgccccgac
     1201 cgcctgctgt atcacgtcga tgacgagggc aagaacgact ggacgccgtc gcaactggcc
     1261 aatgcgctgg ccgggctgtc ggccgagaag gccgcgttcg ccgccagcgg gcagcggttc
     1321 tcgatgctcg tgatgcccga caagtcatcg gtctaccgcg acgacatcgt cgccccgcgc
     1381 atccgcccca gcagcgtcac gcgcgacctg caggcggccg gactgttcgg catggacacg
     1441 atgacgtgct tccgcacgct cgcgacgtcc atgcccgatt tctacctgcc cgacgacacc
     1501 cacgtcggcc cgaacgcctt ccgcctgctg gccgcggcgc tcgcgcacgg ccagcagtgt
     1561 cccgcgccgg cggcgccggc cagcgcggcc gcctcggccg ggctcaattg acgttgtcgt
     1621 agaggccgag ccggtacttg atcgaccggg ccgcgcgcgt cgcggtcgac gtggcgaagg
     1681 tgcggatgcg gtccaggccg atctccaggc cgctctcggg gcgcgccgcg gccggcatgt
     1741 cgacggcgaa gcgctgctgc aggcagtcga tgcgctggcg gctgccgcgc acgtagttct
     1801 cgacgttgga gtcgtggatg acctgcagcc agctcggctc gtcgtcgacg tcgacgatgc
     1861 ggcccagccg cgcgatgtcg gagtgcgagc ccttgtagat cgtgttgaag ccggccgact
     1921 tgccttgcgg cagcggctcg acgagcgtcg tgaacgcgtt gtgctcctgg cgatccaggt
     1981 agacgcggtc cttgtgcagg acgtagccca tcgggaactg cagcaccagc cgctcggtgg
     2041 agcgggcgtg gcgctgcacc ttggcgacgt agtcacgcgc caggccgtcg tcgttgtcca
     2101 ggcgcgtggt cagcagcgtc tgcggcgagt cgtccagata agggccgacc accaacggac
     2161 cgaccgtgct ggtgccgcgg ggcagccaga tcggcaccag gttggaccag tcggccgcgt
     2221 acgcctccac gcgcgagcgg atcgccgcgg gcgtctcgtc ggcgaacagc accagccact
     2281 tgaagtcctg caccgtctgg ctgcgcaccg tcggcaggca gtagcgctcg aacagctcga
     2341 ggcgatgcgc caggtaggcg ctgtcgacca tcttggccga ggacggcgtg tcctcgatcc
     2401 cgcgcaggtt gaagcgcgtg atgatgaagt gccgcaccgt ggcgccgccc ggcgtcgagg
     2461 cgtcggggga agcggggttc gacggtgcgc aaggactgga catcagggcg tgaagaaggc
     2521 gcctacgtcg gaggttcccg cgccgcgcca cgttccggcg aagtcgtcgg tgacggtgaa
     2581 gcccgacggc agggcctgct tggcggactt gcgggcgggg gacgtggacg aggtcgtgaa
     2641 attcggggac gtgagcgaca cgctcgcgaa cagcgggtcg ccggacaggc ccagcagatc
     2701 gccgatggtc gagaacaggt tcggcagctg cgtgccggcg gaatacagat tgttcttgaa
     2761 agagtaggtc gttgtcgagg ccgcggtccc cccagcatcg acgaaccact gggttccggc
     2821 gacggtgtcg gggccgaacg cgaagatgtt gtcgtagaac aggttgttga ccccgctctg
     2881 cgccgaccag ccctccgcgc gcacgtagcc gttgccggtg cccgaggtgg tggtcatgca
     2941 gtcgtacatc gtgttgtagg cgaaccagag ctgctggacg ggctgcgtcg agttgatgcg
     3001 cagcgggggc agggcggtgc cgatgatgac gttgtcccag atcatgccgc tgtaggtgcc
     3061 ggccttggac gactgctggt cgttgaagtt gacgccgtac ttggccgcgt tctgcagcca
     3121 gttgtggtgg atcttgatgc ccgtgaagcc cgcccacgtg ccgccgtgcg gcagggcgta
     3181 gctgccggcg ccgccctcgt tgtcgttgaa ctggatggcg ctgccgccgg tcatgttgtt
     3241 gatccagttg taggcgacct cccagttctg cgccgtggtg tcggcgtaga tgccgtggtt
     3301 ctgcaggtcg gacagcccgg cgatgtcgtg gatgtggttg ccgtagatga gcatcgtgtc
     3361 gccgtggccc gagatgcccg cggcgttcag gacggccgag ttgccggcga cccaggggcc
     3421 cagctcgttg ttgacgacgc gccagtggcc ggcggtgtac tggaagttga tcggcgcggc
     3481 gtcgcgggcc gcgcccccgg ccacgtccat gcgcaggttg gacacggcga tgtactcgcc
     3541 gctggtgccg gcgatggccg accaggggcc ggcgatgccg ccggatgtgc ccgaaggcgt
     3601 cgtgtagtgg acgtcctcga tggcgttgcc cttgatcggg cccgggtagg cggtgatgtg
     3661 gatccacgcc ttggcggtgc cgttgcgcgc ggccgagccg gcgccgaagc gcatccacgt
     3721 cgtgtcgacg ccattggtgt cgttccacgt gccgccccgc agcacgacct ggtcgccggc
     3781 gcccatcttg aagtaggcgc ccgtcgatgt cgtcatgttc tgcatgtagc gcagcgggtg
     3841 cgtgatgtcg cccgccaccg cggtcgaatc gttgccggtg ggcgagacga accagacctt
     3901 gccggaggtc ggcgtgaacg tggcgtccat gttgctgggc acgccgttga cgaccaccag
     3961 caccggcgcc gaggtgccga ccgccaggcc gccgaggttg cccacctgga cggtcaactg
     4021 ctgcagcccc agctggctgc cgaccttggc ggcgcccagg tagcgatagt tggcgacttc
     4081 gctgccgccg atgaagacct tggtggtcgt gcccagcccg ctggccgagc cgaagttcga
     4141 cccgaagatg gacaggtagc cacccaggct gttctcgccg cccgtggtgg gcgcagtcac
     4201 ggtgtcgacg tagcgcaccc agggcgcgcc ggcgacgggc accttgggcg agaagttggc
     4261 cgtcaccgtc ttgctggtgc tgagggtgac cgtgcaggtg gtgctggtgc cgccgcaact
     4321 gccgccccag ccgccgaacg tgaagttggc cgcgggcacg gcggtcagcg tcaccgcggt
     4381 gttggtggcg aggtcggcgc tgcacgtggc ggtgctgcac gaggcgccgt ccgtgg
//
LOCUS       DATCRO010000169         5924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_778260, whole genome shotgun sequence.
ACCESSION   DATCRO010000169 DATCRO010000000
VERSION     DATCRO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778260"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1096)
                     /gene="tal"
                     /locus_tag="VIN75_04610"
     CDS             complement(5..1096)
                     /gene="tal"
                     /locus_tag="VIN75_04610"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY8973477.1"
                     /translation="MNATKQLHDLGQSLWIDNISRETLRDGTLARLIAEYSVTGLTSN
                     PTIFEKAMSEGDAYDDAIARMARDGRSTEDLFFALAVEDLQQAADLFLPAHEASGGVD
                     GWASLEVSPLLVSDTQGTIRTAASLCQQAGRNNLFIKIPGTPEGIPAIEESIYAGVPI
                     NVTLLFSREQYLAAAEAYMKGIERRLNDGKSPLVESVASLFVSRWDTGAKDTVSPEYQ
                     NKLGIAIAMRTYKAYRELLESPRWKKLAAAGAHPQRLLWASTGTKDPKAPETLYVEAL
                     AAPDTIDTMPEKTLKAFAKDGRVGAKMALDGGDAEQTLAELRRQGVDIEALAVKLQKD
                     GGDAFAKSWHALLGGLEEKVAKLTATSPS"
     gene            complement(1093..1794)
                     /locus_tag="VIN75_04615"
     CDS             complement(1093..1794)
                     /locus_tag="VIN75_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8973478.1"
                     /translation="MTASPHDVVFLVDVDNTLLDNDRIVSDLREHLVQAFGAECADCY
                     WARFEQLRAQLGYADYLGALQLYREDVERRGVAAHALLQMSSFLVDYPFRERVFPGAF
                     DALARMARLGPTVILSDGDVVFQPRKIHRSGLWRAVDGRVLIYVHKETMLDELRRAFP
                     ARHYAIVDDKPRILGAFKAAWGAGVTTVFPRQGHYALDAAEVARWPAPDVAVDRIGEL
                     ARTNLAGWIPSRSEP"
     gene            1933..3984
                     /gene="tkt"
                     /locus_tag="VIN75_04620"
     CDS             1933..3984
                     /gene="tkt"
                     /locus_tag="VIN75_04620"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8973479.1"
                     /translation="MSDKLDQQCIDTLRFLSVDMVQKADSGHPGLPLDAAPMAYVLWT
                     RFLKHHPANPMWADRDRFVLSAGHGSALLYSLLNVTGYGVTIDDLKQFRQWGSRTPGH
                     PERGHAPGVEVTTGPLGQGLANAVGLAIAEAQLAGTYNRDGHAVIDHRTWAIVSDGDL
                     MEGVASEAASLAGHLQLGKLVCLYDDNEVTLSAGSDITFTEDKARRFEAYGWQTIKVA
                     DGNDVDAIDRALREAVADTTRPTMILVRTHLGYGSPLQDSFKSHGSPLGDKNVKATKD
                     KLGWPEAPDFFVPDDAARKFGEAQAAGEKAEAAWNERMAAYAKAFPQEHAALQARLRG
                     ELPAGWDADMPVFPADEKGMATRVAGKQILAAIAPRLPALVGGSADLDPSTHTNLVDQ
                     GDFGPKPAADAKPIEGADKGGISWAGRNVHFGVREHAMAAIVNGMAAHGGFIPYGSTF
                     LIFSDYMRPAIRLAALQKLHALHVFTHDSIALGEDGPTHQPIEQLASLRAIPGLTVIR
                     PADANETAVAWRVAVTMKDRPVLLVLTRQDVPTLDRAAHPSADGLAKGAYVIGGAEHD
                     RPDLILIASGSEVSLIVAAAQALGQEGVKVRCVSMPSWDLFEAQPASYREAVLPPSVT
                     RRLAVEAGATQGWERYVGQAGDVIGIDHFGASAPAKVLLKEFGFSADNVIARARRLLV
                     R"
     gene            3984..4970
                     /locus_tag="VIN75_04625"
     CDS             3984..4970
                     /locus_tag="VIN75_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8973480.1"
                     /translation="MAGGDAGSRTAVWAALAGNVLVAATKAGAAAVTGSSAMLSEAIH
                     SFVDTGNEVLLLYGMRRARRAPDRDHPAGYGRELYFWSFVVALLVFALGAGVSIVQGV
                     QRVHHPEPIESPLVSELVLAAAFVFEGWSWAVSVRQFSRAKGDLGWYEAFLRSKDPPL
                     FMVVFEDSAALAGIVIAAAGTVLAAHFDLPLADGVASILIGLVLAGTSTLLLRESKSL
                     LIGERADGELVRSMLAIANAACGNSRANGVLTLHLAPDQVMAAMSFEFDDALTAPQIE
                     QMIADIERRIHDAHPEVTTLFVKPQSPQQYRDRVREKYGDGVEEATRVARDD"
     gene            5106..>5924
                     /locus_tag="VIN75_04630"
     CDS             5106..>5924
                     /locus_tag="VIN75_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY8973481.1"
                     /translation="MAAVPEATATWSRPTRLDAAWLRLRVWRLVALRAWHDLRDARVR
                     RHRAGAALEGAMVVAEHRSPLWRDGREDEFRLVAGKVQNLRVARRAFDGVVVPAGAVL
                     SFWKQLGRPTRGRGFVEGREIRAGCVVPVVAGGLCQLSNALAACALECGMTLVERHAH
                     TARIERDAGAPDAVDATVSWNYVDLRLSAPFDWRLEIELTGDELVVRMRAALPRARGG
                     ASRPIPLAPERGPLPLARGCLTCDEVRCFRHPGVMEPVAAGRTAVLVDTWTPEFA"
BASE COUNT          822 a         2121 c         2119 g          862 t
ORIGIN      
        1 gccgtcaact cggcgacgtc gccgtcagct tggcgacctt ctcctcgagc ccgcccagca
       61 gcgcgtgcca ggacttcgcg aacgcgtcgc cgccgtcctt ctgcagcttg accgccagcg
      121 cctcgatgtc gacgccctgg cggcgcagct cggccagcgt ctgctccgca tcgccgccat
      181 cgagcgccat cttggcgccg acccggccgt ccttggcgaa ggccttgagc gtcttctcgg
      241 gcatcgtgtc gatggtgtcg ggcgcggcca gcgcctcgac gtacagcgtc tcgggggcct
      301 tcggatcctt ggtgcccgtg ctggcccaca gcagccgctg cggatgcgcg ccggcggccg
      361 cgagcttctt ccagcgcggc gactccagca gctcgcggta ggccttgtac gtgcgcatcg
      421 cgatcgcgat gcccagcttg ttctggtact cgggcgacac cgtgtccttg gcgccggtgt
      481 cccagcggct gacgaacagc gaggccaccg attcgaccag cggcgacttg ccgtcgttga
      541 gccggcgctc gatgcccttc atgtaggcct cggccgcggc caggtactgt tcgcgcgaga
      601 acagcagcgt cacgttgatc ggcacgcccg cgtagatcga ctcctcgatc gccgggatgc
      661 cctcgggcgt gccggggatc ttgatgaaca ggttgttgcg gccggcctgc tggcacaggc
      721 tggccgcggt gcggatcgtt ccctgcgtgt cgctgaccag cagcggcgac acctccagcg
      781 aggcccagcc gtccaccccg ccgctggcct cgtgcgccgg caggaacagg tcggcggcct
      841 gctgcaggtc ctcgaccgcc agcgcgaaga acaggtcctc ggtggagcgg ccatcgcgcg
      901 ccatcctggc gatcgcgtcg tcgtatgcat cgccctcgct catggccttc tcgaagatcg
      961 tcgggttgga cgtcaggccc gtgaccgagt actcggcgat cagccgggcg agcgtgccgt
     1021 cgcgcagcgt ctcgcggctg atgttgtcga tccacaggct ctggcccagg tcgtgcagtt
     1081 gcttggtggc gttcatggtt cgctcctgga agggatccaa ccggcaagat tcgtgcgtgc
     1141 gagctcgccg atgcggtcga cggcgacgtc gggcgccggc cacctggcca cctccgcggc
     1201 gtcgagcgcg tagtggccct ggcgcgggaa gacggtcgtc acgccggcgc cccaggcggc
     1261 cttgaacgcg ccgaggatgc gcggcttgtc gtcgacgatc gcgtagtggc gcgcggggaa
     1321 cgcacgccgc agctcgtcga gcatcgtctc cttgtgcacg tagatcagca cgcggccgtc
     1381 gaccgcgcgc catagtcccg agcgatggat cttgcgcggc tggaagacga cgtcgccatc
     1441 cgacaggatc accgtcggcc cgagccgcgc catccgcgcc agcgcgtcga acgccccggg
     1501 gaacacgcgc tcgcggaacg ggtagtcgac caggaagctg gacatctgca gcagcgcgtg
     1561 cgccgccacg ccgcggcgct cgacgtcctc ccggtacagc tgcagcgcgc cgagatagtc
     1621 ggcgtagccc agctgcgcgc gcagctgctc gaagcgcgcc cagtagcagt ccgcgcactc
     1681 ggcgccgaac gcctgcacca ggtgctcgcg caggtcggaa acgatgcggt cgttgtccag
     1741 cagggtgttg tcgacgtcga ccaggaagac cacgtcgtgc ggagaagctg tcatcggaag
     1801 cgggctcgct gaacgaggat tgaaggtgcg ccggcgcggg caagcagctt gccgctccag
     1861 tctccggttc gcacccccgc agagcaaagc ccatacccgg ctgtcgcctg tcacctcgcc
     1921 tgaagggtcc ccatgtccga caagctcgac cagcaatgca tcgacacgct ccgcttcctg
     1981 tccgtggaca tggtgcagaa ggccgacagc ggccacccgg ggctgccgct cgatgcggcg
     2041 ccgatggcct acgtgctgtg gacgcgcttc ctcaagcatc acccggccaa cccgatgtgg
     2101 gccgaccgcg atcgcttcgt gttgtcggcc ggccacggct cggcgctgct ttacagcctg
     2161 ttgaacgtga cgggctacgg cgtcaccatc gacgacctca agcagttccg ccagtggggc
     2221 agccgcacgc cgggccaccc cgagcgcggc catgcgcccg gcgtcgaggt gaccaccggc
     2281 ccgctgggcc agggcctggc caacgccgtg ggcctggcca tcgccgaggc acagctggcc
     2341 gggacctaca accgcgatgg acacgccgtg atcgaccacc gcacctgggc catcgtcagc
     2401 gacggcgacc tgatggaagg cgtcgcgtcc gaggccgcgt cgctggccgg gcacctgcag
     2461 ctgggcaagc ttgtttgcct gtacgacgac aacgaggtca cgctgtcggc cggctcggac
     2521 atcacgttca ccgaggacaa ggccaggcgc ttcgaggcct acggctggca gacgatcaag
     2581 gtggccgacg gcaacgacgt cgacgcgatc gaccgcgcgc tgcgcgaggc cgtggccgac
     2641 acgacgcggc cgaccatgat cctcgtgcgc acgcacctgg gctacggctc gccgctgcag
     2701 gacagcttca agtcgcacgg ctcgccgctg ggcgacaaga acgtcaaggc caccaaggac
     2761 aagctgggct ggcccgaggc gcccgacttc ttcgtgcccg acgatgcggc ccgcaagttc
     2821 ggcgaggcgc aggccgccgg cgagaaggcc gaggccgcgt ggaacgagcg catggcggcg
     2881 tacgccaagg ccttcccgca ggagcacgcc gcgctgcagg cgcgcctgcg cggcgagctg
     2941 cccgcgggct gggacgccga catgccggtc tttcccgccg acgagaaggg gatggccacg
     3001 cgcgtggccg gcaagcagat cctcgcggcc atcgcgccca ggctccccgc gctggtgggc
     3061 ggctcggccg acctcgaccc gtccacgcac accaacctcg tcgaccaggg cgacttcgga
     3121 cccaagcccg cggccgacgc gaagccgatc gagggcgcgg acaagggcgg catctcgtgg
     3181 gccggccgca acgtgcactt cggcgtgcgc gagcacgcga tggccgcgat cgtcaacggc
     3241 atggccgcgc acggcggctt catcccgtac ggctcgacct tcctgatctt ctcggactac
     3301 atgcgccctg cgatccgcct cgccgcgctg cagaagctgc acgcgctgca cgtgttcacg
     3361 cacgactcga tcgcgctcgg cgaggacggc ccgacgcacc agccgatcga gcagctcgcc
     3421 agcctgcgcg cgatcccggg gctcacggtg atccggccgg cggacgccaa cgagacggcc
     3481 gtcgcgtgga gggtggcggt cacgatgaag gaccggcccg tgctgctcgt gctcacgcgc
     3541 caggacgtgc ccacgctgga tcgcgccgcg catccgagcg cggacggcct cgccaagggc
     3601 gcctacgtca tcggcggcgc ggagcacgac aggccggacc tgatcctgat cgccagcggc
     3661 tcggaggtga gcctgatcgt cgccgccgcg caggcgctcg ggcaggaggg cgtcaaggtg
     3721 cgctgcgtgt cgatgcccag ctgggacctg ttcgaggcgc agcctgcgag ctaccgcgag
     3781 gcggtgctgc cgccgtcggt cacgcgccgc ctggcggtcg aggccggcgc cacgcagggc
     3841 tgggaacgct acgtcggcca ggccggggac gtgatcggca tcgaccactt cggcgcatcg
     3901 gcgccggcca aggtgctgct caaggaattc ggcttcagcg ccgacaacgt catcgcgcgg
     3961 gcgcgccggc tgctcgtccg ctgatggccg gcggcgacgc gggctcgcgc acggccgtct
     4021 gggccgcgct ggccggcaac gtgctcgtgg cggccaccaa ggccggcgcg gccgccgtca
     4081 ccggcagctc ggcgatgctc agcgaggcga tccactcgtt cgtcgacacc ggcaacgagg
     4141 tgctgctgct gtacggcatg cggcgggcgc gccgcgcgcc cgaccgcgac catcccgccg
     4201 gctacggacg cgagctgtac ttctggagct tcgtggtcgc gctgctggtg ttcgcgctcg
     4261 gcgcaggcgt gtccatcgtg cagggcgtgc agcgcgtgca ccatccggag cccatcgaga
     4321 gcccgctggt cagcgaactc gtgctcgccg cggcgttcgt gttcgagggc tggtcgtggg
     4381 cggtgtcggt gcgccagttc agccgggcca agggcgatct cggctggtac gaggccttcc
     4441 tcaggagcaa ggatccgccg ctgttcatgg tcgtcttcga ggacagcgcc gcgctggcag
     4501 gcatcgtcat cgccgcggcg ggcacggtgc ttgcggcgca cttcgacctg ccgctggccg
     4561 acggcgtcgc gtcgatcctg atcgggctgg tgctggcggg cacgtcgacg ctgctgctgc
     4621 gcgagagcaa gagcctgctg atcggcgagc gcgcggacgg cgagctggtg cgctcgatgc
     4681 tggccatcgc caacgcggcc tgcggcaact cgcgcgcgaa cggcgtgctc acgctgcacc
     4741 tggcgcccga ccaggtgatg gccgcgatga gcttcgagtt cgacgacgcg ctcaccgcgc
     4801 cgcagatcga gcagatgatc gccgacatcg agcgccgcat tcacgacgcc catcccgagg
     4861 tgacgaccct gttcgtcaag ccgcagagcc cgcagcagta ccgcgaccgg gtgcgcgaga
     4921 aatacggcga cggcgtggag gaggcgacac gcgtggcgcg cgacgactga ggcgtgggtg
     4981 ctacgcttgc gctccattcg caactcgccg gcgccgcgcc gcttccgcca tgggtctttg
     5041 gagtcgactt ttcggcggat cggtgccacg acgtgatgcc gtgccgttga aggatgacgc
     5101 gcgcgatggc cgcggtgcct gaagccacgg ccacctggtc ccgcccgacg cgcctggacg
     5161 ccgcctggct gcggctgcgc gtctggcgcc tcgtcgcgct gcgcgcctgg cacgacctgc
     5221 gcgacgcgcg cgtgcggcgc catcgcgccg gcgcggcgct ggaaggcgcg atggtcgtcg
     5281 ccgagcaccg ttcgccgctg tggcgcgacg gccgcgagga cgagttccgg ctggtggccg
     5341 ggaaagtgca gaacctgcgc gtcgcgcggc gcgcgttcga cggcgtcgtc gtcccggcag
     5401 gcgccgtgct cagcttctgg aagcagctcg ggcggccgac gcgcgggcgc ggcttcgtcg
     5461 agggccgcga gatccgcgcc ggctgcgtcg ttcccgtggt cgccggcggg ctctgccagc
     5521 tgtcgaacgc gctcgcggcc tgcgcgctcg aatgcggcat gacgctcgtg gaacgccatg
     5581 cgcacacggc gcgcatcgag cgcgacgccg gcgcgccgga cgcggtcgat gcgacggtgt
     5641 cctggaacta cgtcgacctg cggctgtcgg cgccgttcga ctggcggctg gagatcgagc
     5701 tgaccggtga cgagctcgtc gtgcgcatgc gcgccgcgct gccgcgcgcc cgcggcggcg
     5761 cgagccggcc gatccccctg gcgcccgaac gcggcccgct gccgctcgcg cgcggctgcc
     5821 tcacctgcga cgaggtgcgc tgcttccgcc atcccggcgt catggagccg gtcgcggccg
     5881 gccgcaccgc cgtgctggtc gacacctgga caccggagtt cgcg
//
LOCUS       DATCRO010000170         7440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_586934, whole genome shotgun sequence.
ACCESSION   DATCRO010000170 DATCRO010000000
VERSION     DATCRO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586934"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VIN75_04635"
     CDS             <1..772
                     /locus_tag="VIN75_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY8973482.1"
                     /translation="PGAGDAGFDLAAFDERLLADDLRRLAAGVPEPLQAAMLAALGPA
                     PGPERLLDLALRVGPYGDRFGQKPGGLSLAALKDAPHGVDLGALAPRLPDILRTPSGR
                     IELWPAPVAAELAAVDAALADAPPALVVVGRRDVRSNNSWMHNLPTLAKGPERCTALL
                     NPDDAARHGVRTGDRVRLQGAAGRAIELPALLTDEMMPGVVSVPHGWGHDQAGARLGV
                     AAQRPGANLNELLDDRLRDPLSGTSVLNGIPVTLSRCA"
     gene            complement(805..1500)
                     /locus_tag="VIN75_04640"
     CDS             complement(805..1500)
                     /locus_tag="VIN75_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8973483.1"
                     /translation="MNTPPIPGSAPLLEVAGLAKRYGDAFVFSDVSLVMQRGELVALL
                     GESGVGKSTLLNCVAGLDTADAGRIALAGRDVLGLSEPEAARLRREDLGFVFQAFHVL
                     PHLNVADNVGLPLQLLGRPRGDRVARMLAAVGLDGLGERAPRQLSGGQLQRVAIARAL
                     VHEPALVLADEPTGNLDPRSAGLVMDVLVDAARRTGAGVLMVTHSPPAAARCDRVLEL
                     TAQGVRLPATAPA"
     gene            1835..2605
                     /locus_tag="VIN75_04645"
     CDS             1835..2605
                     /locus_tag="VIN75_04645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973484.1"
                     /translation="MSGLVKVLERRSTRFWAGAAIASALAGIAVATGLGMLMGGTGGL
                     IALSVAVFFALAALVLAGVVIRLARVVADTRARQALQAVDDDLTGLSNRRHFLELVER
                     EFARCRRYGDDGALLLIDADHLRRINEHLGQLCGDALLLEVTKRVGTTLRQPDLLARF
                     GGAELAVFLPNTDPLGALDAAERIRQQVASAPYTWNQTHVVATVSIGVATVHQGQLSV
                     DTMVHEAEQALEAAKEAGRNCVRTGPIKPRKNGERVST"
     gene            2641..2880
                     /locus_tag="VIN75_04650"
     CDS             2641..2880
                     /locus_tag="VIN75_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973485.1"
                     /translation="MGASGFFMAGCQNLCRRIFRNHLSGIATGAFGSTSPERPPTLPG
                     LRAAVEATDAWYLAFVSAIVDEIPASARARAAHPP"
     gene            2968..4422
                     /locus_tag="VIN75_04655"
     CDS             2968..4422
                     /locus_tag="VIN75_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8973486.1"
                     /translation="MPVDHVFVLMLENRSFDHLFGLTQRQATPIPAAADFAAGAPDPC
                     PTDPAHEFDDVQAQINGGAMDGFKDPLQRKGFAADKLPALNELAGRYVLFDNWFSSVP
                     GPTWPNRFFVHAASSGGLCTSPTALRTTGAIIAAGEHFQFDNGTLFDLFDRKFPGAPA
                     WRVYHGDVTPQCLALPGMVRKYYQGPTYFRNLYPSTAPDGTPRDDGFASDVMSSAGYA
                     PRYTFIEPNYALQLIASNDFTTGDSMHPCGRAGAGDNLVRYVHDTLSGSPIWDRSVLL
                     VLWDEHGGFYDHVAPQAATPPGDVEHNRAKGQDGADFKFDRLGVRVPALLIGPTVPAG
                     ALGSELFPGRSFDHASVISTMRSLFQLGGPLTNRDRDAPTWDGCLAAGQAADAAPLTS
                     RALVPSRRKSGATPAPAAVDGFATGMGLIAMDLDRVGAALSGQPTIGDFSPRTVADFH
                     AARDATLAAGNFPEVLGRYMGAVQERAASVKAAA"
     gene            complement(4452..4988)
                     /locus_tag="VIN75_04660"
     CDS             complement(4452..4988)
                     /locus_tag="VIN75_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973487.1"
                     /translation="MVWLTLTFAVLLGAMAIITFLTIGAIRADTHPAVHRARGLVDVV
                     KPSRRSPLFRLSRPGAEPLQFKCLSEVIASPRCPAASPDWTPREMTVEWIDVPTLPRL
                     VGARATRMAVADDPLFAASPRDVLDGEMANLRSGLPELGRILGGMMLVSAAVAAFLFG
                     RLSFVTRRIEAAHRRAGA"
     gene            complement(5096..6076)
                     /gene="miaA"
                     /locus_tag="VIN75_04665"
     CDS             complement(5096..6076)
                     /gene="miaA"
                     /locus_tag="VIN75_04665"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8973488.1"
                     /translation="MTDSRDSTAAPRWLGLAGPTASGKTALALALARRWPVEIVSVDS
                     ALVYRGMDIGSAKPSAQERAAAPHHLLDIIDPAQSYSAAQFVADARRLIGEIRARGRW
                     PLLVGGTMMYFKALADGIDEMPAADPAIRAAIDAEAAQHGWPAMHARLAEVDPVTAAR
                     LKPNDSQRIQRALEVWRVSGTPLSALHQRAQRDAGATDGALLALEPASRAWLHARIAQ
                     RFDAMLDAGFLDEVRRLRADAALNLDLPSMRCVGYRQAWEALDAADPPDVAGLRERGI
                     AATRQLAKRQITWLRSMGHRQVVEADAEDVEARLVAAARSLLGPFGSSPT"
     gene            complement(6073..>7440)
                     /locus_tag="VIN75_04670"
     CDS             complement(6073..>7440)
                     /locus_tag="VIN75_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8973489.1"
                     /translation="DLPSNVWRADPPPAAPSPSDRPPYERRDGPRRAHGDAPRPDRPG
                     ERRTSPGPWQDTAPRYEFRDRPDERRSGPPPQGERRRDAAPPGDREAPTRERPRFEDR
                     ERPRHEERDRPRFDDRDRGPYGERGRGPSYPQGPNERPRNLNSRGDERAPHGYAAERG
                     RPERGDRRPSGPGDRPPRRRDDVAPEAFAPRPPQAERAARPAFDPDAPRREDQRLSKV
                     LAERGIASRREADEWIEAGWVKVDGEVATLGARVFPHQVIEVDEAAKTQQARRVTILL
                     HKPVGYVSGQAEDGYEPASVLVTPDNHWPEDPSRERWNAGHGRHLAPAGRLDIDSTGL
                     LVLTQDGRIARHLIGDESDVEKEYLVRVEYPGGDQFPDADLQRLRHGLWLDGHELRPA
                     KVSWANEDQLRFVLREGRKRQIRRMCEAVGLVVTGLKRVRIGSVVLGKLPPGQWRYLR
                     DDERF"
BASE COUNT          979 a         2735 c         2722 g         1004 t
ORIGIN      
        1 acccggcgcc ggcgacgccg gcttcgacct ggcggccttc gacgagcgcc tgctggccga
       61 cgacctgcgc cggctggccg cgggcgtccc cgagccgctg caggccgcga tgctggccgc
      121 cctcggtccg gcgccgggcc ccgagcgcct gctggatctc gcgttgcgcg tgggccccta
      181 cggcgaccgc ttcggccaga agccgggcgg cctgagcctg gccgcgttga aggacgcccc
      241 gcacggcgtg gacctcggcg cgctcgcgcc acggctgccc gacatcctgc gcacccccag
      301 cggccggatc gagctgtggc cggcgccggt cgccgcggag ctggccgccg tcgacgccgc
      361 cctggccgac gcgccgccgg cgctggtggt ggtgggccgg cgcgacgtgc gctcgaacaa
      421 cagctggatg cacaacctgc ccacgctggc caagggcccc gagcgctgca ccgcgctgct
      481 gaatccggac gacgccgcgc gccatggcgt gcgcaccggc gaccgcgtgc ggctgcaggg
      541 cgcggcgggt cgcgcgatcg agctgccggc gctgctcacc gacgagatga tgcccggcgt
      601 ggtcagcgtg ccccacggct ggggccacga ccaggcgggc gcgcgcctgg gcgtcgcggc
      661 gcagcggccc ggcgccaacc tcaacgagct gctggatgac cggctgcggg accctctgtc
      721 cgggacgtcg gtgctgaacg gcattccggt gacgctgagc cgctgcgcgt agcggcgcag
      781 cgagtccggg caggcggtga tgcgtcaggc cggagccgtc gcgggcagcc gcacgccctg
      841 ggcggtcagc tccagcaccc ggtcgcagcg ggcggccgcc ggtggcgaat gggtcaccat
      901 cagcacgccg gcgccggtgc ggcgggcggc gtccaccagc acgtccatca ccagtccggc
      961 gctgcggggg tcgaggttgc ccgtgggctc gtcggccagc accagggccg gctcgtgcac
     1021 gagtgcgcgc gcgatcgcca cccgttgcaa ctggccaccc gagagctggc gcggcgcgcg
     1081 ttcgcccagg ccgtccaggc cgaccgcggc cagcatgcgc gccacgcggt cgccgcgcgg
     1141 gcgtccgagc aactgcagcg gcaggcccac gttgtcggcc acgttcaggt gcggcaggac
     1201 gtggaacgcc tggaacacga agcccaggtc ctcgcggcgc aggcgggccg cctcgggttc
     1261 ggacagcccc agcacgtcgc gcccggccag ggcgatgcgg ccggcgtcgg cggtgtccag
     1321 gccggccacg cagttgagca acgtcgactt gcccacgccc gattcgccca gcagggccac
     1381 cagctcgccc cgctgcatca cgagcgagac gtcgctgaag acgaaggcgt cgccgtagcg
     1441 cttggcgagg ccggcgacct cgaggagagg ggcggagccg ggaatcggtg gcgtgttcat
     1501 ccggcgccaa gcatacggga tgcggcatgc tcgacagcgc tgacagcgga acttcgcacg
     1561 ccgggtgccg gccgccacga acaacctcac catttacgtg attttgggcc gccggatgct
     1621 gtcgcgtttg cgggctctgc cgcacaatcg gtcaaagttt ccgctcatgg gcagtccgcg
     1681 gcggccgata gtgtctgaag tgtcgtcgtc ctccctgggc gcgcacgtcc aagcctggcc
     1741 gcaagagccg ggcgctgtat ttgcccgcga cctgtccggc gggcatgaca tctcctgagg
     1801 tggaagggtg acggctgaaa tcgattcctc gacgttgagc gggctcgtga aggtgctcga
     1861 gcgtcgcagc acccgcttct gggcgggcgc cgcgatcgcc tctgcgctgg ccggcatcgc
     1921 cgtcgccacc ggcctgggca tgctcatggg cggcacgggc ggcctgatcg cgctgagcgt
     1981 cgccgtgttc ttcgcgctgg ccgcgctggt gctggcgggc gtcgtcatcc ggctggcccg
     2041 cgtcgtggcc gacacccgcg cgcgccaggc gctccaggcc gtcgacgacg acctgaccgg
     2101 actgtccaac cgccgccact tcctcgaact ggtcgagcgc gagttcgccc gctgccgtcg
     2161 ctacggcgat gacggcgcgc tgctgctgat cgacgcggac cacctgcggc gcatcaacga
     2221 acacctgggc cagctgtgcg gcgacgcact gctgctcgaa gtcaccaagc gcgtgggcac
     2281 gacgctgcgc cagcccgacc tgctggcccg cttcggcggc gccgaactgg cggtcttcct
     2341 gcccaacacc gacccgctgg gcgcgctcga tgccgccgag cgcatccgcc agcaggtcgc
     2401 gtccgcaccg tacacctgga accagaccca tgtcgtggcc accgtcagca tcggcgtggc
     2461 caccgtccac cagggccagc tgagcgtcga caccatggtg cacgaggccg aacaggctct
     2521 cgaggccgcc aaggaagccg gccgcaactg cgtgcgcacc ggcccgatca agccacgcaa
     2581 gaacggggag cgcgtgtcga cctgagtgcc cgacacgctc gcgaaagccg ctgccgccgg
     2641 atgggtgcca gcggcttttt catggctggc tgccagaacc tgtgccgtcg catcttccgc
     2701 aaccacctgt ccggcatcgc caccggggcc ttcggatcga cgagtcccga gcgtcctccc
     2761 accttgcccg gactgcgcgc ggcggtcgag gcgaccgacg cgtggtatct cgccttcgtc
     2821 tccgcgatcg ttgacgaaat tcctgcatcg gccagagccc gggcggcgca tccaccctga
     2881 atgaacgtgg cggaccgccg gccatcgtga gaacctcctg ccgcaacaag ggcctgtccg
     2941 tggcctggaa cccgaggaga gaaaccgatg cccgtcgacc acgtcttcgt cctgatgctg
     3001 gagaaccggt ccttcgacca cctgttcggc ctgacccagc gccaggcgac gccgattcct
     3061 gccgccgccg atttcgccgc gggcgcaccc gatccctgtc cgacggaccc cgcgcacgag
     3121 ttcgacgacg tccaggcgca gatcaacggc ggggcgatgg acggattcaa ggatccgctg
     3181 cagcgcaagg gcttcgctgc cgacaagctg cccgcgttga acgagctcgc gggccgttac
     3241 gtcctgttcg acaactggtt ctcgtccgtg cccggcccga cctggccgaa ccggttcttc
     3301 gtccacgccg cctcgagcgg cggcctctgc acgagtccca cggcgctgcg caccaccggt
     3361 gcgatcatcg ccgccggcga gcacttccag ttcgacaacg gcacgctgtt cgacctgttc
     3421 gacaggaagt tccccggggc gcctgcctgg cgcgtttacc acggcgacgt cacgccgcag
     3481 tgcctggcgc tgccgggcat ggtgcgcaag tactaccagg gcccgacgta cttccgcaac
     3541 ctgtatccga gcacggcgcc ggacgggaca ccgcgcgacg acggcttcgc gtcggacgtg
     3601 atgtccagcg cgggatatgc cccccggtac accttcatcg aaccgaacta cgccctgcag
     3661 ctgatcgcca gcaacgactt cacgacgggc gactcgatgc atccgtgcgg gcgtgccggc
     3721 gccggggaca acctggtgcg ctacgtccat gacacgctga gcggctcgcc gatctgggac
     3781 aggagcgtgc tgctggtcct ctgggacgag cacggcggct tctacgatca cgtcgcgccc
     3841 caggccgcga cgccgcccgg cgacgtcgag cacaaccgcg ccaagggcca ggacggcgca
     3901 gacttcaagt tcgaccggct cggcgtgcgg gtgcctgccc tgctgatcgg gccgacggtg
     3961 cccgcgggcg cgctgggcag cgaactgttc cccggccggt cgttcgacca cgcgagcgtc
     4021 atcagcacga tgcgaagcct gttccagctc ggcggaccct tgaccaaccg ggaccgcgac
     4081 gcgccgacgt gggatggctg cctcgcggca ggccaggccg ccgatgccgc cccgctgaca
     4141 tcgcgtgcgc tcgtcccgag tcgacgcaag tccggcgcca cgccggcgcc ggccgcggtc
     4201 gacggattcg ccacgggcat gggcctgatc gcgatggacc tcgaccgggt cggcgcggcg
     4261 ctgagcggcc agccgaccat cggcgacttc agccccagga ccgtcgccga cttccacgcc
     4321 gcgcgcgacg ccacgcttgc cgccggcaac ttcccggagg tgctcggccg ctacatgggg
     4381 gccgtgcagg agcgggcggc gtccgtcaag gctgcggcct gatcctcggg cggccaggca
     4441 accgccgcgg gtcaggcgcc ggctcgccga tgcgccgcct cgatccggcg cgtcacgaac
     4501 gacaggcggc cgaacaggaa tgccgcgacg gcggcgctca cgagcatcat tccgccgagg
     4561 atcctgccca gttccgggag gcccgaccgg aggttcgcca tctcgccgtc caggacgtcg
     4621 cgcggcgaag cggcaaagag cgggtcgtcg gccacggcca tgcgcgtggc ccgcgcgccg
     4681 accaggcgcg gcagcgtggg cacgtcgatc cattcgaccg tcatctcgcg cggcgtccaa
     4741 tctggcgagg ccgccgggca gcgcgggctg gcgatcacct cggacaggca cttgaactgc
     4801 agcggttcgg cgccgggccg cgagagccgg aacagggggc tgcgccggct gggcttgacg
     4861 acatcgacga ggccgcgcgc ccgatgcacc gccggatgcg tgtccgcgcg aatggcgccg
     4921 atcgtcagga acgtgatgat cgccatcgcc ccgagcagca ccgcgaaggt cagggtgagc
     4981 cagaccatgc tgcgggcatt gttgcgcagg ctgtcctgct ccggggagtc cgccctgcgt
     5041 cctggcgctt tccagcgggc gtcgccgggg cgccccggcg gaagatagga ggccgtcatg
     5101 tcggagaact cccgaatggt ccgagcagcg atcgtgccgc ggccaccagc cgcgcctcga
     5161 cgtcctctgc atccgcctcg acgacctgcc ggtgccccat gctgcgcaac cacgtgatct
     5221 gccgcttggc gagctggcgc gtcgccgcga tgccgcgttc gcgcagtccg gccacgtccg
     5281 gcggatcggc cgcgtccagc gcctcccagg cctggcggta gccgacgcag cgcatcgagg
     5341 gcaggtccag gttcagcgcg gcgtcggcgc gcaggcggcg cacctcgtcg aggaagccgg
     5401 cgtccagcat cgcgtcgaag cgttgcgcga tgcgcgcgtg cagccatgcg cgtgacgcgg
     5461 gctcgagcgc gagcagggcg ccgtccgtcg cgccggcgtc gcgttgcgcg cgctggtgca
     5521 gcgccgacag cggcgtgccg ctcacgcgcc acacctccag cgcgcgctgg atgcgctgcg
     5581 agtcgttggg cttgagccgc gcggcggtga ccgggtcgac ctcggccagc cgcgcgtgca
     5641 tcgcgggcca gccatgctgc gcggcttcgg cgtcgatcgc ggcgcggatc gcggggtcgg
     5701 cggccggcat ctcgtcgatg ccgtcggcga gcgccttgaa gtacatcatc gtgccgccca
     5761 ccagcagcgg ccagcggccg cgcgcgcgga tctcgccgat gaggcggcgc gcgtcggcca
     5821 cgaactgcgc ggcggaatac gactgcgcgg gatcgatgat gtcgaggagg tggtgcggcg
     5881 ccgcggcccg ctcctgggcg ctcggcttgg cgctgccgat gtccatgccg cgatagacca
     5941 gcgccgagtc gacgctgacg atctccaccg gccagcgccg cgcgagcgcc agcgcgagcg
     6001 ccgtcttgcc ggacgcggtg ggcccggcga gcccgagcca gcgcggcgcg gcggtcgagt
     6061 cgcggctgtc ggtcaaaacc gttcgtcgtc gcgcaggtag cgccactggc cgggcggcag
     6121 cttgcccagc acgacgctgc cgatgcgcac gcgcttcagg cccgtgacga ccaggccgac
     6181 ggcctcgcac atgcggcgga tctggcgctt gcggccctcg cgcagcacga agcgcaactg
     6241 gtcctcgttg gcccagctga ccttggccgg acgcagctca tggccgtcca gccacaggcc
     6301 gtggcgcagg cgctgcaggt ccgcgtcggg gaactggtcg ccgccggggt actcgacgcg
     6361 caccaggtat tccttctcga cgtccgactc gtcgccgatc aggtggcgcg cgatgcggcc
     6421 gtcctgggtc agcaccagca ggccggtgga gtcgatgtcc agccggccgg ccggcgcaag
     6481 atggcggccg tggcccgcgt tccagcgctc gcgcgacgga tcctcgggcc agtggttgtc
     6541 gggcgtgacc agcaccgacg ccggctcgta gccgtcctcg gcctggccgc tgacgtagcc
     6601 cacgggcttg tgcagcagga tggtgacgcg gcgcgcctgc tgcgtcttgg cggcctcgtc
     6661 gacctcgatg acctggtgcg ggaagacgcg cgcgcccagc gtggcgacct cgccgtcgac
     6721 cttgacccag ccggcctcga tccactcgtc ggcctcgcga cgcgacgcga tgccgcgctc
     6781 ggccagcacc ttggacaggc gctggtcctc gcgcctgggg gcgtccggat cgaacgcggg
     6841 acgcgccgcc cgttcggcct gcggcgggcg cggcgcgaag gcctcgggcg cgacgtcgtc
     6901 gcggcggcgc ggcgggcggt cgccggggcc ggacgggcgg cggtcgccgc gctcggggcg
     6961 gccgcgttcg gcggcatagc cgtgcggcgc gcgctcgtcg ccgcgcgagt tcagattgcg
     7021 cggccgttcg ttggggcctt gcggatagct cgggccgcgg ccgcgctcgc catacggacc
     7081 gcggtcgcgg tcatcgaaac ggggacgatc ccgctcctcg tggcgcgggc gctcgcgatc
     7141 ctcgaaacgc gggcgctcgc gcgtcggggc ctcgcgatcg ccgggcggcg ccgcgtcgcg
     7201 gcggcgctcg ccctggggcg gcggcccgga gcggcgctcg tcggggcgat cgcggaactc
     7261 gtagcgcggc gccgtgtcct gccaggggcc ggggctggtg cgacgctcgc cggggcggtc
     7321 gggacgcggc gcgtcgccgt gcgcacggcg cgggccgtcg cggcgctcgt acggcggccg
     7381 gtcggaaggc gacggcgcgg ccggcggcgg gtcggcgcgc cagacgttgg acggcaggtc
//
LOCUS       DATCRO010000171         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_991869, whole genome shotgun sequence.
ACCESSION   DATCRO010000171 DATCRO010000000
VERSION     DATCRO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991869"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VIN75_04675"
     CDS             <1..615
                     /locus_tag="VIN75_04675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY8973490.1"
                     /translation="AYYDPTKNEIVFPAAILQPPFFDMKADDAANYGAIGAVIGHEIS
                     HGFDDEGSQFDGDGKLRNWWTASDRKAFDAITSKLDAQYSAYEPLPGVHVKGKLTMGE
                     NIADLSGLQIAYKAWQISLNGQPAPVIDGVSGAQRFYYGFAQVWREKDRDEAMLQLVT
                     SNPHSPPQFRADGAAINSDGFHEAFATKPGDKMWKAPADRLRLW"
     gene            747..2825
                     /locus_tag="VIN75_04680"
     CDS             747..2825
                     /locus_tag="VIN75_04680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY8973491.1"
                     /translation="MPQPTFSLTLLAAALACAALAPAQAQAQDAARSTKTVRTITTMK
                     LGSGIDRAGMDKSVRPQDDLYLAMNGTWVKNTQIPPDKAIWGAFIDLRELSDKRVHEI
                     VEGLQAASPAPGSNAQKVNDYFRAYLDEAGIDKAGLAPVEASLEDVDSIKDAGMLAGL
                     MGHWQGVVSMPLNVGGYPDYDDPTVYIAHVQQGGLGLPDRDYYLKSDERMAKARTAYV
                     DYLGRLFSLSGDADAAAHAAQVMALETRIATAQWARDRTRDPNLAHNPKTPAELEALG
                     PGFDWPTFEAQSQLQPGRVVNVRQPDYVTALAGLVKSEPLDAWKLYLKARRLDGAATM
                     LPAAFRDASFQFHGVAISGLKEPPPRWQDGVGATNGALGEAVGQLYVARYFPPAYRAR
                     MQALVANLMKAYSTSIDNLAWMSPATKVQAHAKLAKYGVKIGYPDVWRDYSALDVRAG
                     DALGNADRAAAFEYHRTIVRIGGKVDRTEWGMTPQTVNAYYNGNKNEIVFPAAILQPP
                     FFNVAADDATNYGAIGAVIGHEISHGFDDKGSQLDGDGRLRNWWTDEDRKAFDEITGR
                     LVAQYSAYEPLDGQHLNGKLTLGENIADLSGLQIAYKAWRMSLGGRKAPVIDGLTGEQ
                     RFVYGFAQVWRTKIRDEEALKRIATDPHSPGRFRADGATINADVFHDAFHTKPGDRMW
                     KAPEDRLRLW"
     gene            2888..3526
                     /locus_tag="VIN75_04685"
     CDS             2888..3526
                     /locus_tag="VIN75_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEY8973492.1"
                     /translation="MTTPPPLPAEARARVRALVKRSGRVLLGLVGPPGAGKSTLAAAL
                     LAEFARIAQVVPMDGFHLANIELQRLGRRGRKGAPDTFDAHGYVALLRRLLAQGDDEI
                     VYAPEFRREIEEPVAGAIAVLPRTQLVITEGNYLLLEDGAWGAVSGLLDETWYVDIPD
                     ALRVERLTQRHVQFGRSADDAAAWVAGTDEPNARLIEASRGRASFVFRWDDA"
     gene            3659..4078
                     /locus_tag="VIN75_04690"
     CDS             3659..4078
                     /locus_tag="VIN75_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8973493.1"
                     /translation="MNAKQWFAAAVAATGAAGALASQSPLQTEDPSAGKPATVVAWDP
                     SCAPQYPVAAVQSGAQGVTRVAVHLDEGGKVVSVDIIQRSGNTREHHMLDSEAARAIS
                     RCPFTPARDATGKAIPSVVTLEQEWHVDGAQASAAGR"
BASE COUNT          673 a         1494 c         1464 g          544 t
ORIGIN      
        1 gcgtactacg atccgaccaa gaacgagatc gtgttcccgg cggccatcct gcaacccccg
       61 ttcttcgaca tgaaggccga cgacgcggcc aactacggcg cgatcggcgc ggtcatcggc
      121 cacgagatca gccacggctt cgacgacgag ggcagccagt tcgacggcga cggcaagctg
      181 cgcaactggt ggacggcgtc cgaccgcaag gccttcgacg ccatcaccag caagctcgac
      241 gcgcagtact cggcctacga gccgctgccc ggcgtgcacg tcaagggcaa gctgacgatg
      301 ggcgagaaca tcgccgacct gtcgggcctg cagatcgcgt acaaggcctg gcagatctcg
      361 ctgaacggcc agccggcccc ggtgatcgac ggcgtcagcg gcgcgcagcg cttctactac
      421 ggcttcgcgc aggtctggcg cgagaaggat cgcgacgagg cgatgctgca gctggtcacc
      481 agcaacccgc actcgccccc gcagttccgc gccgacggcg ccgccatcaa cagcgacggc
      541 ttccacgagg cgttcgccac caagccgggc gacaagatgt ggaaggcccc ggccgaccgt
      601 ctgcgcctct ggtaagccgc gcgaggtcgc tgccgtgacc accggttcct gaagtccgag
      661 ggctgcgccg atacgcgcgg ccctttttca ttcctgcgcg acaggtcgcg ccccgttccc
      721 ttccgcttgc cgcacgaggt tccgtcatgc cccaacccac cttcagcctc accctgctcg
      781 cggccgcgct cgcgtgcgcg gcgctcgcac ccgcgcaggc gcaggcgcag gacgccgccc
      841 ggtcgacgaa gacggtcagg accatcacca ccatgaagct gggctcgggc atcgaccgcg
      901 ccggcatgga caagtccgtg cgcccgcagg acgacctgta cctggcgatg aacggcacct
      961 gggtcaagaa cacgcagatc ccgccggaca aggcgatctg gggcgcgttc atcgacctgc
     1021 gcgaactcag cgacaagcgc gtgcacgaga tcgtcgaggg cctgcaggcc gcgagtcccg
     1081 cgccgggcag caacgcgcag aaggtcaacg actacttccg cgcctacctc gacgaggccg
     1141 ggatcgacaa ggccggcctc gcgccggtcg aggcttcgct cgaggacgtc gacagcatca
     1201 aggacgcggg catgctcgcg ggcctgatgg gccactggca gggcgtcgtg tcgatgccgc
     1261 tgaacgtcgg cggctatccg gactacgacg atccgacggt ctacatcgcg catgtgcagc
     1321 agggcggcct ggggctgccc gaccgcgact actacctgaa gtccgacgag cgcatggcca
     1381 aggcgcgcac cgcctacgtc gactacctgg gcaggctgtt ctcgctgtcc ggcgacgccg
     1441 acgccgccgc gcatgccgcg caggtgatgg cgctggagac caggatcgcg accgcgcaat
     1501 gggcccgcga caggacgcgc gacccgaacc tggcgcacaa cccgaagacg cccgcggaac
     1561 tcgaggcgct ggggcccggc ttcgactggc ccaccttcga ggcgcagtcg cagttgcagc
     1621 ccgggcgcgt cgtcaacgtg cggcagcccg actacgtgac ggcgttggcg gggctggtca
     1681 agtccgagcc gctcgacgcg tggaagctgt acctgaaggc gcgccgcctg gacggcgccg
     1741 ccacgatgct gcccgccgct ttccgcgatg cgagcttcca gttccacggc gtggcgatca
     1801 gcggcctgaa ggagccgccg ccgcgctggc aggacggcgt cggcgcgacc aacggcgcgc
     1861 tcggcgaggc cgtcggccag ctctacgtgg ccaggtactt cccgccggcc taccgcgcgc
     1921 gcatgcaggc gctggtcgcc aacctgatga aggcgtattc cacgtcgatc gacaacctcg
     1981 cctggatgag cccggccacc aaggtgcagg cccatgccaa gctcgcgaag tacggcgtca
     2041 agatcggcta tccggacgtg tggcgcgact actcggcgct cgacgtcagg gccggcgacg
     2101 cgctcggcaa cgccgaccgc gccgccgcgt tcgagtacca ccgcaccatc gtgcgcatcg
     2161 gcggcaaggt cgatcgcacc gagtggggca tgacgccgca gaccgtcaac gcgtactaca
     2221 acggcaacaa gaacgagatc gtgttccccg cggccatcct gcagccgccg ttcttcaacg
     2281 tggccgccga cgacgcgacc aactacggcg cgatcggcgc ggtgatcggc cacgagatca
     2341 gccacggctt cgacgacaag ggcagccagc tcgatggcga cggccgcctg cgcaactggt
     2401 ggacggacga ggaccgcaag gccttcgacg agatcaccgg ccgcctggtg gcgcagtact
     2461 cggcctacga gccgctggac ggccagcatc tgaacggcaa gctgacgctg ggcgagaaca
     2521 tcgccgacct gtcgggcctg cagatcgcgt acaaggcctg gaggatgtcg ctgggcggca
     2581 ggaaggcgcc ggtcatcgac ggcctgacgg gcgagcagcg cttcgtctac ggcttcgcgc
     2641 aggtctggcg cacgaagatc cgcgacgagg aggcgctcaa gcgcatcgcc accgacccgc
     2701 actcgccggg ccgcttccgc gcggacggcg ccacgatcaa cgccgacgtg ttccacgacg
     2761 cgttccacac caagccgggc gacaggatgt ggaaggcgcc ggaggaccgg ctgcgccttt
     2821 ggtgagcgcg gcgcgcatcg cccgcgcgtg acgcgcgcgc gctgcgcggc cctatgatgg
     2881 cgacgccatg accacgccgc ccccacttcc cgccgaagcg cgcgcccgcg tccgcgcgct
     2941 cgtcaagcgc agcggccgcg tgctgctggg cctggtcggt ccgccgggcg cgggcaagtc
     3001 cacgctggcc gcggcgctgc tggccgagtt cgcgcgcatc gcccaggtgg tgccgatgga
     3061 cggcttccac ctggccaata tcgaactgca gcgcctcggg cggcgcgggc gcaagggcgc
     3121 gcccgatacc ttcgacgcgc acggctacgt cgcgctgctg cgccggctgc tggcgcaggg
     3181 cgacgacgag atcgtctacg cgcccgagtt ccggcgcgag atcgaggagc cggtcgcggg
     3241 tgcgatcgcc gtcctgccgc gcacgcaact ggtcatcacc gaaggcaact acctgctgct
     3301 ggaggacggt gcctggggcg ccgtctcggg cctgctggac gagacctggt acgtcgacat
     3361 ccccgacgcg ctgcgcgtcg aacgcctgac gcagcgccac gtgcagttcg gccgcagcgc
     3421 tgacgacgcg gcggcctggg tggccggcac cgacgagccg aacgcgcggc tgatcgaggc
     3481 gtcgcgcggc cgcgcgtcgt tcgtgttccg ctgggacgat gcctagttca cggaaggcgg
     3541 cggcgcgtgg cgcttcgccc atgggcaacc gggcccggat gtggtccagt ccacgccgag
     3601 ggtcgccgca ggccgcgggt ggtttcgagg cgccgacgat acgaacagga gtcgaacgat
     3661 gaatgcgaaa cagtggttcg cggcggcggt tgccgcgacg ggcgccgccg gcgccctggc
     3721 gtcgcagtcg ccgctgcaga cggaagatcc ctcggcgggc aagccggcca cggtcgtggc
     3781 ctgggacccc agctgcgcgc cgcagtatcc ggtggcggcc gtccagtccg gcgcccaggg
     3841 cgtgacgcgc gtggccgtcc acctggacga gggcggcaag gtggtgtccg tcgacatcat
     3901 ccagcgctcc ggcaacaccc gcgagcacca catgctcgac agcgaagccg cccgcgcgat
     3961 ctcgcgctgc ccgttcacgc ccgcccgcga cgcgaccggc aaggcgatcc ccagcgtcgt
     4021 cacgctggag caggagtggc acgtggacgg ggcgcaggcg agcgcggcgg ggcgctgacg
     4081 cttcggacgt ctccggctcg ccggagcggg atgcgttcga gcaggggtgg atccggcgac
     4141 tccgcttcgc ttcgcgcagg atgacgggct ggcgc
//
LOCUS       DATCRO010000172         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_193143, whole genome shotgun sequence.
ACCESSION   DATCRO010000172 DATCRO010000000
VERSION     DATCRO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193143"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VIN75_04695"
     CDS             complement(<1..1743)
                     /locus_tag="VIN75_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8973494.1"
                     /translation="MSDAQAPEPPIDSSIPPYVPPEADGDVRAVLRTPWARRWALVVL
                     MLMVAWTLVAWWVNTRHYEERVAGVVASATAQADASATLVEDGLRQGLAQLDGVAHML
                     TRLPMIRRAVSAAGPGAAIVDEPVAQLRSRLQAQPDLAAVDDFLASSSEELGADLIYL
                     LDDAGNCVAASNAGTAGSVVGAHLNEPAFFYQTRDEHGARAFAVGKATGIPGMYFSAA
                     IYDDGRFKGIVVVKRNTRSLAAMVGHEGAFVVDALGVVIMSQDPRLLLQALSGAHTSQ
                     LKPAERAARYRSELLPVLRIGPWEGGGPDALRRIGDDPAPQVLVQRHMEREALDVYVS
                     QRVPEVENLARERGFNFALMALAGAGAIAIVAAGLGGWRSRRSVIDAVQRIGQANHKL
                     SVEMAERERAEVLLRESREYLLVHEARLRALLRSLPEQVWMKDRAGRYLAVNAEFERF
                     QGVSEAEVLGKSDFDMGTWERSEHYAAQDQATLAASEPLMFLETEHSAAGGGERHLEV
                     TKTAVRDDRGEVIGVVGIARDMTEIHAANLALKTSEARFRATFETAGDAILIIRHAVI
                     IDCNRRAAEMLGAGT"
     gene            complement(1867..>3763)
                     /locus_tag="VIN75_04700"
     CDS             complement(1867..>3763)
                     /locus_tag="VIN75_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973495.1"
                     /translation="LDVDLDTGAAFVTDRAKFGQVVLNLLGNALKFTDAGRIVLRAWR
                     AGGDTVVEVEDTGPGMDASEVADLFTALLTALLAARRRRDEVMQEARDHLEFQVQART
                     RDLASTNTRLRDEIDGHRRTEALLQDARQHAEAASRAKSQFLANMSHEIRTPLNAVLG
                     YTQLLIEDRRQAPETRERLRVIHAAGQRLLGLINDVLDLAKIEAGALQVHMAPMDLRH
                     ELGEIFSLFEPRAEAKGLHLRLDVDLDTGAAFVTDRAKFGQVVLNLLGNALKFTDAGR
                     IVLRAWRAGGDTVVEVEDTGPGMDEGEVADLFTAFRQGAAGIDKGGTGLGLNLSRHIA
                     QALGGELAVTSQKGRGTTVRLRLPMAEVDRAGVPAAAFAGGQRLAPGTSLRVLVVEDD
                     PYSRDVLVTLLRETGCDVESAVDGEAGLAACRARPADAPYDIVFTDIRMPRLDGLQML
                     QALRADERTRGLPLVAVSASSLEHERRYYVTHGFQDFVGKPYEFGAIHEMLAQHAGAR
                     LVPAPADDAAPPPALESAAEVVDRLQRGAIAAAGIVRRSVVRAQLSALADGAASGSMT
                     QVRDQLAMLSAVPARALPAGLLAQIEGDLRQYDFAALEGRVREALAREMAGDDDGEDV
                     TSKEAGA"
BASE COUNT          476 a         1424 c         1357 g          506 t
ORIGIN      
        1 cgtgccggcg cccagcatct cggcggcgcg ccggttgcag tcgatgatga cggcgtggcg
       61 gatgatcagg atcgcgtcgc ccgccgtctc gaaggtggcg cggaagcgcg cctcgctggt
      121 cttgagcgcg aggttggcgg cgtggatctc cgtcatgtcg cgcgcgatgc cgaccacgcc
      181 gatgacctcg ccgcggtcgt cgcgcaccgc ggtcttcgtg acctcgagat ggcgctcgcc
      241 accgccggca gccgagtgct ccgtctcgag gaacatcagc ggctcggacg cggcgagcgt
      301 ggcctggtcc tgcgcggcgt agtgctcgga gcgctcccac gtgcccatgt cgaagtcgga
      361 cttgcccagc acctccgcct cgctcacgcc ctggaagcgc tcgaactccg cgttgaccgc
      421 caggtagcgg cccgcgcgat ccttcatcca gacctgctcg ggcaggctgc gcagcagcgc
      481 gcgcagccgc gcctcgtgca ccagcaggta ctcgcgcgac tcgcgcagca gcacctcggc
      541 gcgctcgcgc tcggccatct ccaccgacag cttgtggttg gcctggccga tgcgctgcac
      601 cgcgtcgatc acgctgcggc gcgagcgcca gccgcccagg cccgcggcca cgatcgcgat
      661 cgccccggcg cccgccagcg ccatcagcgc gaagttgaag ccgcgctcgc gcgccaggtt
      721 ctcgacctcg ggcacgcgct gcgacacgta cacgtccagc gcctcgcgct ccatgtggcg
      781 ctgcaccagc acctggggcg ccggatcgtc accgatgcgc cgcagcgcgt ccgggccgcc
      841 gccctcccag ggcccgatgc gcagcacggg cagcagctcg gagcggtagc gcgccgcgcg
      901 ctcggcgggc ttgagctgcg acgtgtgcgc acccgacagc gcctgcagca gcagccgcgg
      961 atcctgcgac atgatcacga cgcccagcgc atccacgacg aaggcgccct cgtggcccac
     1021 catcgcggcc agcgaacgcg tgttgcgctt gacgacgacg atccccttga agcggccgtc
     1081 gtcgtagatg gccgccgaga agtacatgcc cgggatgccc gtggccttgc ccacggcgaa
     1141 cgcccgcgcg ccgtgctcgt cgcgcgtctg gtagaagaac gcgggctcgt tcaggtgcgc
     1201 gcccaccacg ctgcctgcag tgccggcgtt gctggccgcc acgcagttgc cggcatcgtc
     1261 gagcaggtag atgaggtcgg cgccgagctc ctcggacgac gaggcgagga agtcgtcgac
     1321 cgccgcgagg tcgggctgcg cctgcagccg gctgcgcagc tgggcgaccg gctcgtcgac
     1381 gatcgccgcg ccgggccccg cggccgagac cgcgcgacgg atcatgggca gccgggtcag
     1441 catgtgggcg acgccgtcga gctgcgccag gccctggcgc agcccgtcct cgaccagcgt
     1501 ggcgctggcg tcggcctgcg ccgtggccga ggcgacgacg ccggccacgc gctcctcgta
     1561 gtggcgcgtg ttgacccacc acgcgaccag cgtccacgcg accatcagca tcagcaccac
     1621 cagcgcccag cgccgcgccc agggcgtgcg cagcacggcg cgcacgtcgc cgtcggcctc
     1681 gggcggcacg tagggcggga tggacgagtc gatgggcggc tcgggcgcct gggcgtcgct
     1741 catgaggcaa cctccccgcc acgcgctccg gtcgattcgc ctttccggcg gccgtgcggg
     1801 ctcataaggc aagctccgcc ctgcgggctg cggtgcgagc gcccttcgca gcggccgggc
     1861 gggcactcat gcgcccgcct ccttcgacgt gacgtcctcc ccgtcgtcat cgccggccat
     1921 ctcgcgcgcc agcgcctcgc gcacacggcc ctccagcgcc gcgaagtcgt actggcgcag
     1981 gtcgccctcg atctgggcga gcaggcccgc gggcagtgcg cgcgccggca ccgcggacag
     2041 catcgccagc tggtcgcgca cctgcgtcat cgagccggag gccgcgccgt cggccagcgc
     2101 cgagagctgc gcgcgcacga cgctgcgccg cacgatgccg gccgcggcga tcgcgccgcg
     2161 ctgcagccgg tcgaccacct cggccgcgct ctcgagcgcc ggcggcggcg ccgcgtcgtc
     2221 ggccggcgcg ggcaccagcc gcgcgcccgc atgctgcgcg agcatctcgt ggatcgcgcc
     2281 gaactcgtag ggcttgccga cgaagtcctg gaaaccgtgc gtgacgtagt agcggcgctc
     2341 gtgctcgagg ctggacgccg acaccgcgac cagcggcagc ccgcgcgtgc gctcgtcggc
     2401 gcgcagcgcc tgcagcatct gcaggccatc caggcgcggc atgcggatgt cggtgaagac
     2461 gatgtcgtag ggcgcatcgg ccggacgcgc gcggcaggcg gccaggcccg cttcgccgtc
     2521 gacggcggac tcgacgtcgc agcccgtctc gcgcagcagc gtcaccagca cgtcgcggct
     2581 gtacgggtcg tcctcgacca ccagcacgcg caggctcgtg cccggagcca gccgctggcc
     2641 gccggcgaac gccgcggccg gcacgccggc gcggtcgacc tcggccatcg gcagccgcag
     2701 ccgcacggtg gtgccgcgcc ccttctggct cgtgaccgcg agctcgccgc cgagcgcctg
     2761 cgcgatgtgg cgcgacaggt tcaggcccag gcccgtgccg cccttgtcga tgccggcggc
     2821 gccctgtcgg aaggccgtga acaggtcggc gacctcgcct tcgtccatgc cgggaccggt
     2881 gtcctcgacc tcgaccaccg tgtcgccccc ggcgcgccag gcgcgcagga cgatccggcc
     2941 ggcgtcggtg aacttcagcg cgttgcccag caggttgagc acgacctggc cgaacttggc
     3001 gcggtcggtg acgaaggcgg cgccggtgtc gaggtcgacg tccagccgca ggtgcagccc
     3061 cttggcctcg gcgcgcggct cgaacagcga gaagatctcg ccgagctcgt ggcgcaggtc
     3121 catcggcgcc atgtgcacct gcagcgcgcc ggcctcgatc ttggccaggt cgagcacgtc
     3181 gttgatcagc cccagcaggc gctggccggc ggcatggatc acccgcagcc gctcgcgcgt
     3241 ctcgggcgcc tgtcgccggt cctcgatcag cagctgcgtg tagcccagca cggcgttgag
     3301 cggcgtgcgg atctcgtggc tcatgttggc caggaactgc gacttcgcgc ggctggcggc
     3361 ctccgcgtgc tggcgcgcgt cctgcagcag ggcctcggtg cggcggtggc cgtcgatctc
     3421 gtcgcgcagc cgcgtgttgg tgctggcgag gtcgcgcgtg cgggcctgca cctggaactc
     3481 gaggtggtcg cgcgcctcct gcatcacctc gtcgcggcgg cggcgcgcgg ccagcagcgc
     3541 ggtcagcaag gcggtgaaca ggtcggcgac ctcgctcgca tccatgccgg ggccggtgtc
     3601 ctcgacctcg accaccgtgt cgccgccggc gcgccaggcg cgcagcacga tccggccggc
     3661 gtcggtgaac ttcagcgcgt tgcccagcag gttgagcacg acctggccga acttggcgcg
     3721 gtcggtgacg aaggcggcgc cggtgtcgag gtcgacgtcc agc
//
LOCUS       DATCRO010000173         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1048082, whole genome shotgun sequence.
ACCESSION   DATCRO010000173 DATCRO010000000
VERSION     DATCRO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048082"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VIN75_04705"
     CDS             <1..1934
                     /locus_tag="VIN75_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY8973496.1"
                     /translation="TNLLAPRFPMLLADYARRVAERYPDQRLWTPVNEPLTTARFSGL
                     YGLWYPHAADDRSFVRALLHQVQATVLAMRAIREVVPDAQLVQTDDLGHTRCTPALAD
                     QAAFDNERRWLAFDLLAGRVGAAHPLREWLCGCGAGEDELDRLVESPCPPDILGINAY
                     ITSERFLDERLELHPPHLHGGNGREAYVDIETARGHGGLIDGFAGRLREAHARYGLPL
                     AITEVHLGCTREEQMRWLKQAWDAACQARRDGIDVRAVTCWAAFGTVDWSSLLTREDG
                     HYEPGLWDARSEPPRLTALGRLASQLAAGADERSLHPVLASPGWWQRDLRLHAHPHGA
                     VTALPARGATLLVLGTNALGRAFARLCHMRGLPYEMLSRAQVDAADETDVAEALLRHR
                     PWAVVNATGDARVDAAEADELQWRSNAEAPAVLARACRAAGARLLTFSSDYVFPGNRR
                     IPWLEHDPVGPVNAYGHAKARGEAAALEALPDALVVRSAAFFGPWSHDNFVARGLALL
                     RQGRAWFAACDQVVSPTYLPDLVQASLDLLIDGESGLWHLTNGQALTWADFARDAARH
                     AGLDGSLVQAVPGLELGQVARRPAYSALDSARGRLLPTLDDALARYQRDHEPIPAPVP
                     PLRSFVEEAKERSGPSPAH"
     gene            2011..>3230
                     /locus_tag="VIN75_04710"
     CDS             2011..>3230
                     /locus_tag="VIN75_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8973497.1"
                     /translation="MNQASELRPAPSANPVDDGLYRNVWRHAAGARTALAGSMLLLVA
                     SQVVKLCVPWCAAKAIDVLQAGGPGMLAHALAWVAGVIGAYVGSWLLHGPGRVLERNV
                     GMRVRRSLADTLYGRLAGAPLSWHESHHSSEMQQRAGQATGALYDFAQNQFVYLHSIV
                     NFVGPLVALALLSPVLGFAALAGYVTLAALNARFDRSLMRLSLKEMDLERRYAAGMSD
                     FLGHMGSVLSLRLQAATRVLLGRRMEAAFEPLARIIGLTEWKWCMVDLVSTALTWSLV
                     IGQVLLSTHHVTTGLAIGAIFMVYQYAGKTADVASAMAGQLQGLQHTRTNFESARPIF
                     DAPQSPAPVALPADWQRIVVRDVSFRHAKAHPDAPSLGVAELVLNRGERIALVGGSGS
                     GKSSLMRVLAGLYEA"
BASE COUNT          414 a         1206 c         1141 g          469 t
ORIGIN      
        1 acacgaacct gctcgcgccg cgcttcccga tgctgcttgc ggattacgcg cgccgcgtcg
       61 ccgaacgcta tcccgaccag cgcctctgga cgcccgtcaa cgagccgctg acgacggcgc
      121 gcttctccgg cctgtacggc ctgtggtatc cgcacgctgc cgacgaccgc agcttcgtgc
      181 gcgctctgct gcaccaggtg caggccaccg tgctggccat gcgcgccatc cgcgaggtcg
      241 tgcccgacgc gcagctggtg cagaccgacg acctcggcca cacgcgctgc accccggcgc
      301 tcgcggacca ggccgcgttc gacaacgagc gccgctggct cgcgttcgac ctgctcgcgg
      361 gccgcgtcgg cgccgcgcat ccgctgcgcg agtggctgtg cggctgcggc gcgggcgagg
      421 acgaactcga tcgcctcgtc gaatcgccct gcccgcccga catcctgggc atcaacgcct
      481 acatcaccag tgaacgcttc ctcgacgagc ggctcgagct gcatccgccg cacctgcacg
      541 gaggcaacgg ccgcgaggcc tacgtcgaca tcgagacggc gcgcggccac ggggggttga
      601 tcgacggctt tgccgggcgc ctgcgcgagg cccatgcgcg ctacggcctg ccgctggcga
      661 tcaccgaggt gcacctgggc tgcacgcgcg aggagcagat gcgctggctc aagcaggcct
      721 gggacgccgc gtgccaggcg cgccgcgacg gcatcgacgt gcgcgccgtc acgtgctggg
      781 cggccttcgg cacggtcgac tggtccagcc tgctgacgcg cgaggacggc cactacgagc
      841 cgggcctgtg ggacgcgcgc agcgaaccgc cgcgcctgac ggcgctgggc cgcctcgcat
      901 cgcagctcgc ggcgggcgcc gatgaacgat cgctccaccc cgtgctcgcc tcgcccggtt
      961 ggtggcagcg cgacctgcgc ctgcatgcgc atccgcacgg ggccgtgacg gcgctgccgg
     1021 cgcgcggcgc gacgttgctg gtcctgggca ccaatgcgct gggccgcgcg ttcgcgcgcc
     1081 tttgccacat gcgcggcctg ccctacgaga tgctgtcgcg cgcgcaggtc gatgccgccg
     1141 acgagacgga tgtggccgag gcgctgctgc gccaccgtcc gtgggccgtg gtcaacgcga
     1201 ccggcgacgc gcgcgtggat gctgccgagg ccgatgagct gcagtggcgg tcgaacgccg
     1261 aggcccccgc ggtgctcgcg cgggcctgcc gcgcggccgg cgcgcggctg ctgacgttct
     1321 cgtccgacta cgtcttcccg ggcaatcgcc gcatcccgtg gctcgagcac gatcccgtcg
     1381 gtccggtcaa cgcgtacggc catgccaagg cgcgcggcga ggcggcggcg ctcgaagcgc
     1441 tgcccgatgc gctggtcgtt cgttcagccg cgttcttcgg accgtggagc cacgacaact
     1501 tcgtcgcgcg cggcctcgcg ctgctgcgcc agggccgcgc gtggttcgcc gcgtgcgacc
     1561 aggtcgtctc gcccacctac ctgcccgacc tggtgcaggc cagtctcgac ctgctcatcg
     1621 acggcgaatc gggcctgtgg cacctgacga acgggcaggc cctgacctgg gccgatttcg
     1681 cgcgcgacgc ggcacgccac gccggcctgg acggatcgct cgtgcaggca gtgccgggcc
     1741 tcgagctcgg gcaggtggcg cgccggcccg cgtacagcgc gctggacagc gcgcgcggcc
     1801 ggctgctgcc aacgctggac gacgcgctcg cgcgctacca gcgcgaccac gagccgatac
     1861 cggcgccggt accaccgctg cggtcgttcg tcgaggaagc aaaggaacga tcaggcccct
     1921 cgccggccca ttgatccagc tttcccgacg cggctcgggc ggtatcgtgt caacgggcgt
     1981 cgacatggtc tcgttgtacg ccccgtgcgc atgaaccaag cctccgaact ccgtcccgcc
     2041 ccatccgcca accccgtcga cgacggcctg taccgcaacg tctggcgcca cgccgccggc
     2101 gcccgcacgg cgctggccgg ctcgatgctg ctgctggtgg cctcgcaggt cgtcaagctg
     2161 tgcgtgccct ggtgtgccgc caaggcgatc gacgtgctgc aggccggcgg ccccggcatg
     2221 ctggcgcacg cgctggcctg ggtggccggc gtgatcggcg cctacgtcgg ctcctggctg
     2281 ctgcacggcc cgggccgcgt gctcgagcgc aacgtgggca tgcgcgtgcg ccgctcgctg
     2341 gccgacacgc tgtacggccg tctggccggc gcaccgctga gctggcacga gagccaccat
     2401 tcgagcgaga tgcagcagcg ggcaggccag gccaccggcg cgctgtacga cttcgcgcag
     2461 aaccagttcg tctacctgca cagcatcgtc aacttcgtcg gcccgctggt cgcgctcgcg
     2521 ctgctgtcgc cggtgctggg cttcgccgcg ctggcgggct acgtcacgct ggccgcgctg
     2581 aacgcgcgtt tcgaccgctc gctgatgcgc ctgtcgctca aggagatgga cctcgagcgc
     2641 cgctatgcgg cgggcatgtc cgacttcctc ggccacatgg gcagcgtgct gagcctgcgc
     2701 ctgcaggcgg ccacccgcgt gctgctgggc aggcgcatgg aagccgcgtt cgaaccgctg
     2761 gcgcgcatca tcggcctgac cgaatggaaa tggtgcatgg tcgacctcgt cagcacggcg
     2821 ctgacctggt cgctggtcat cggccaggtg ctcctgagca cgcaccacgt gaccaccggt
     2881 ctcgcgatcg gcgcgatctt catggtctac cagtacgcgg gcaagacggc cgacgtcgcc
     2941 agcgcgatgg ccggccagct tcagggcctg cagcacacgc gcacgaactt cgagagcgcg
     3001 cgcccgatct tcgatgcgcc gcagagcccg gccccggtcg cgctgccggc cgactggcag
     3061 cgcatcgtcg tgcgcgacgt gtcgttccgc cacgccaagg cgcaccccga cgcgccgtcg
     3121 ctgggcgtcg ccgagctggt gctgaaccgc ggcgagcgca tcgcgctggt gggcggcagc
     3181 ggttcgggca agagctcgct gatgcgcgtc ctggccggcc tgtacgaggc
//
LOCUS       DATCRO010000174         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1014254, whole genome shotgun sequence.
ACCESSION   DATCRO010000174 DATCRO010000000
VERSION     DATCRO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014254"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="cysQ"
                     /locus_tag="VIN75_04715"
     CDS             <1..690
                     /gene="cysQ"
                     /locus_tag="VIN75_04715"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY8973498.1"
                     /translation="TDFHVEIKDDESPVTVADRYAEKVIVDGLNRLAPGIPVVGEEGV
                     SAGDIPDVSNRFWLVDPVDGTKEFVRRNGEFTVNIALVDHGVPVLGLVLAPALGTLYV
                     GAAGLGAWVEVGGLRREMAVRQVPADGLTVVGSRHHGNDTAVDAFLAGRAVAANRMVG
                     SSLKLCILAEGLADVYPRFGRTMEWDIAAGDAILRAAGGAVQTLDGAPLRYGKPGFEN
                     PDFVAWADPPR"
     gene            complement(687..2072)
                     /locus_tag="VIN75_04720"
     CDS             complement(687..2072)
                     /locus_tag="VIN75_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8973499.1"
                     /translation="MTDLSALARMRARVTQAVKGDGGGLAARVIRGGSWTLAGNFGGQ
                     ILRFLSNIWLTHLLFPAAFGVMAIAQSIIAGAKMLSDVGLQQSVIRSHRGHDEEFLNT
                     VWTLQVLKGLGILVVMTAIAPLASRLYGQPDLARVIPGLGLAVLISGFASPKVALLNR
                     NIEIGRIVSMELGTQLVGILVMGLWAWLSPTLWSLVAGNIVTALAMTLATHVFIPGPA
                     SRFAWQKSTVQEVWSFGGWVMLSSGMTYLVGEGRNLLNGSLVGPHVIGLMVISTMLVM
                     VLWNAIQSISGRVLFPAYATVWRERPQNLPAVVERSRRIQLLAGCAIAILFALLGDRI
                     VNLFYDARYREAGAFIQIQAAGSIVGFLGGTYGGVLWAIGRPGVSTVLLASQLLATVA
                     LLVVGHAVGGVLGLVVGMSLIGVAMYPVNALVYARFGLFQPRTDALPFLIAIALGAYV
                     FLFGAWRGMSF"
     gene            complement(2147..3022)
                     /locus_tag="VIN75_04725"
     CDS             complement(2147..3022)
                     /locus_tag="VIN75_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY8973500.1"
                     /translation="MKLYFYRGNHENFGDELNHWLMPKVFPEFFDDDPSVLFLGIGSI
                     ILDTHPKDVLKVVYGSGYAGYSDVPRIDDKWKFYCVRGARTAEACGLSPDLVAGDTAI
                     LINRHLQLDRHEKAYKFSYMPHWQSVDVGHWQLVCRWSGVHFIDPRRPVDEVLDELQK
                     TEVLVTEAMHGAIVADALRVPWIPVQPAVTMHRGKWLDWASALDLELDPVVLGASSTY
                     EANVSTPLTGGLGALARAGMPGRKLANVALTTRAANALQRATRRAPSLSTDAALGRAL
                     DKLETAAHQLRRDFA"
     gene            complement(3164..5992)
                     /gene="metH"
                     /locus_tag="VIN75_04730"
     CDS             complement(3164..5992)
                     /gene="metH"
                     /locus_tag="VIN75_04730"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8973501.1"
                     /translation="MSPAENAAAGAQTRIAPPMKLSGLEPVTLDAGHLFVNIGERTNV
                     TGSKAFARMILAGEFEQALAVARQQVENGAQVIDVNMDEAMLDSQAAMVRFLNLVASE
                     PEIARVPVMVDSSKWSVIEAGLKCLQGKGIVNSISMKEGVDEFKRQARLIRRYGAAAV
                     VMAFDEQGQADTYQRKIEICERAYRVLVDEVGFPPEDIIFDPNIFAIATGIEEHDNYA
                     VDFIEATRWIKQNLPGAKVSGGVSNVSFSFRGNEPVREAIHTVFLYHAIQAGMDMGIV
                     NAGMIGVYDNLDPTLRTLVEDVVLNRKPTLAPGEDAASAKTPTERLLDAAEAVKGAVR
                     DDSARLAWRAGTVDERLSHALVQGITDFIVQDTEEAWQAISAKGGRPLHVIEGPLMAG
                     MNVVGDLFGAGKMFLPQVVKSARVMKSAVAHLVPYIEEEKKRMEAAGDDVRSKGKIVI
                     ATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVSCKDILDRAKVEGADIIGLSGLITP
                     SLEEMQYVASELQRDPDFRALKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRA
                     VGVCADLLSEEKGALFREELEADYARVRELHANRKQVPLVTLAAARANRTPLDWAAYT
                     PPAPKFIGRRLFKQYDLAEIAACIDWGPFFQTWDLAGPYPNILTDDVVGEAATKVFAD
                     GQAMLKRVIEGRWLIANGVMGFWPANTVDDDTIEVYTDESRSQVLLKWAPLRMQTERP
                     VVPGEDGVDVRRPNRSLADFIAPKGTKPDWIGLFAVTAGLGVDKHEAAFEAAHDDYSS
                     IMLKAIADRLAEAFAERLHQRVRTELWGYAPDEKLSNEDLIAEKYRGIRPAPGYPACP
                     EHGVKRDLFRALQADEAGMALTESLAMTPAASVSGFYLAHPASTYFNVGRIGEDQVKA
                     WAASVGVSEDEARRRLSQSYEPGSAPPG"
BASE COUNT          918 a         2109 c         2172 g          964 t
ORIGIN      
        1 accgacttcc acgtcgagat caaggacgac gaatcgcccg tcaccgtggc cgaccgctat
       61 gccgagaagg tgatcgtcga tggcctgaac cggctggcgc cgggcattcc ggtcgtgggc
      121 gaggagggcg tatcggccgg cgacattccg gatgtgagca accgcttctg gttggtcgat
      181 ccggtcgacg gcacgaagga gttcgtccgc cgcaacggcg agttcaccgt caacatcgcg
      241 ctcgtcgacc atggcgtgcc ggtgctgggc ctcgtgctcg cgccggcgct gggcacgctg
      301 tacgtgggcg ccgccgggct gggcgcctgg gtcgaagtcg gcggcctgcg ccgcgagatg
      361 gcggtccgcc aggtgcccgc cgacgggctg accgtcgtgg gcagccgcca tcatggcaac
      421 gacaccgcgg tggatgcctt cctcgccggg cgcgcggtcg ccgcgaaccg gatggtcggc
      481 tcgtcgctga agctgtgcat cctggccgag ggcctggccg acgtctatcc gcgcttcggc
      541 cgcacgatgg agtgggacat cgccgccggc gacgccatcc tgcgcgcggc cgggggcgcg
      601 gtgcagacgc tcgacggcgc gccgttgcgc tacggcaagc ccgggttcga gaatcccgac
      661 ttcgtcgcct gggccgaccc gccgcgctag aagctcatgc cgcgccaggc gccgaacagg
      721 aagacgtagg cgcccagcgc gatggcgatc aggaagggca gggcgtcggt gcgtggctgg
      781 aacagcccga agcgcgcgta cacgagcgcg ttgaccgggt acatggcgac gccgatgagc
      841 gacatgccca ccaccagccc cagcacgccg ccgaccgcat ggcccacgac cagcagcgcc
      901 accgtggcga gcagttgcga cgcgagaaga acggtgctga cgcccggcct cccgatcgcc
      961 cacagcacgc cgccgtaggt gccgcccagg aagccgacga tgctgcccgc cgcctggatc
     1021 tggatgaacg cgcccgcctc gcggtagcgc gcgtcgtaga acaggttgac gatgcggtcg
     1081 cccagcagcg cgaacaggat cgcgatggcg cagccggcca gcagctggat gcggcgcgag
     1141 cgttcgacga cggccggcag gttctgcggc cgctcgcgcc agaccgtggc gtacgccggg
     1201 aacagcacgc ggccgctgat cgactggatc gcgttccaca gcaccatgac cagcatcgtc
     1261 gagatcacca tcaggccgat gacgtgcggc ccgacgagcg agccattgag caggttgcgg
     1321 ccttccccga cgagataggt catgccgctg gacagcatca cccagccgcc gaacgaccac
     1381 acctcctgca ccgtcgactt ctgccaggcg aagcggctcg ccgggcccgg aatgaatacg
     1441 tgcgtggcca gcgtcatcgc cagcgcggtg acgatgttgc ccgccaccag cgaccacagc
     1501 gtgggcgaca gccacgccca cagccccatc accaggatgc ccaccagctg cgtgccgagc
     1561 tccatcgaga cgatgcggcc gatctcgatg ttgcgattca gcagcgccac cttgggcgat
     1621 gcgaacccgc tgatcagcac cgccaggccc aggcccggga tgacgcgtgc caggtcaggc
     1681 tggccgtaga gccggctggc gagcggcgcg atcgcggtca tgacgaccag gatgcccagg
     1741 cccttgagca cctgcagcgt ccacaccgtg ttgaggaatt cctcgtcgtg gccgcgatgg
     1801 ctgcggatca cgctctgctg caggccgacg tcggacagca tcttggcgcc cgcgatgatg
     1861 gactgcgcga tggccatcac gccgaacgcc gcggggaaca gcaggtgcgt cagccagatg
     1921 ttgctcagga agcgcaggat ctggccgccg aagttgccgg cgagcgtcca gctgccgccg
     1981 cggatcacgc gcgccgcgag gccgccgccg tcgcccttca cggcctgggt gacccgcgca
     2041 cgcattcgcg cgagggcgga aaggtctgtc atcggagcgg ccacggcgct ggagggagct
     2101 gaagattcta gcggcgcggc gccgcgcacg tccccctcca tcgggctcag gcgaagtcgc
     2161 gccgcagctg gtgcgcggcg gtctcgagct tgtcgagggc gcgccccagg gcggcgtcgg
     2221 tgctgaggct gggcgcgcgt cgagtcgcgc gctgcagcgc gttggcggcg cgggtggtga
     2281 gcgccacgtt ggcgagcttg cgcccgggca tgcccgcgcg cgcgagcgcc cccaggccgc
     2341 cggtcagcgg cgtggacacg ttggcctcgt aggtcgacga cgcgcccagc accaccgggt
     2401 cgagctccag atcgagcgcc gatgcccagt ccagccactt cccgcggtgc atggtcacgg
     2461 cgggttgcac ggggatccag ggcacccgca gcgcgtcggc cacgatggcg ccgtgcatcg
     2521 cctccgtcac gagcacctcg gtcttctgca gttcgtccag cacctcgtcg accgggcggc
     2581 gcgggtcgat gaagtgcacg ccgctccagc ggcacacgag ctgccagtga cccacgtcga
     2641 cgctctgcca gtgcggcatg tacgagaact tgtaggcctt ctcgtggcga tcgagctgca
     2701 ggtggcggtt gatcaggatg gcggtgtcgc cggcgacgag gtcgggcgac aggccgcagg
     2761 cctcggccgt cctggcgccg cgaacgcagt agaacttcca cttgtcgtcg atgcgcggca
     2821 cgtcggagta gccggcatag cccgagccgt agacgacctt caggacgtcc ttgggatggg
     2881 tgtccaggat gatcgagccg atgcccagga acaggacgga cgggtcgtcg tcgaagaact
     2941 ccggaaagac tttcggcatc agccagtgat tcagctcgtc gccgaagttc tcgtggttgc
     3001 cgcgatagaa atacaacttc atcggtaagg atccgctttc ccgggggacg tctcgtcgag
     3061 gcgatgcgcc tgcgcacggc ggcgcgcggg cagccatgag gcgtccgcgc gacctgcgag
     3121 cgcgcaggag catttttcgc gcctgcctcg gcaggcgaca acttcaacct gggggggcgg
     3181 atcccggctc gtacgactgg ctcaggcgcc ggcgcgcctc gtcctcgctg acgccgacgc
     3241 tggccgccca cgccttgacc tggtcctcgc cgatgcggcc cacgttgaag tacgtggacg
     3301 ccgggtgcgc caggtagaag ccggacacgc tggccgccgg cgtcatcgcc aggctctcgg
     3361 tcagcgccat gccggcctcg tcggcctgga gcgcgcggaa caggtcgcgc ttgacaccgt
     3421 gctccgggca ggccggatag cccggcgccg ggcggatgcc gcggtacttc tcggcgatca
     3481 ggtcctcgtt ggacagtttc tcgtcgggcg cgtagcccca cagctcggtg cgcacgcgct
     3541 ggtgcaggcg ctcggcgaag gcctcggcga ggcggtcggc gatggccttg agcatgatgg
     3601 acgagtagtc gtcgtgcgcg gcctcgaacg cggcctcgtg cttgtccacg cccaggccgg
     3661 cggtgaccgc gaacaggccg atccagtcgg gcttcgtgcc cttgggggcg atgaagtcgg
     3721 ccagcgagcg gttcgggcgc cgcacgtcga cgccgtcctc gccgggcacc accggacgct
     3781 cggtctgcat gcgcagcggg gcccacttca gcagcacctg gctgcgcgat tcgtcggtgt
     3841 agacctcgat ggtgtcgtcg tcgaccgtgt tggcgggcca gaagcccatc acgccgttgg
     3901 cgatgagcca gcggccttcg atgacgcgct tgagcatcgc ctggccgtcc gcgaacacct
     3961 tggtggccgc ctcgccgacg acgtcgtcgg tcaggatatt cgggtacggg ccggcgaggt
     4021 cccaggtctg gaagaacggg ccccagtcga tgcaggcggc gatctcggcc aggtcgtact
     4081 gcttgaacag gcgccggccg atgaacttcg gcgcgggcgg cgtgtaggcg gcccagtcca
     4141 gcggcgtgcg gttcgcgcgc gccgccgcga gcgtgaccag cggcacctgc ttgcggttgg
     4201 cgtgcagctc gcgcacgcgg gcgtagtccg cctcgagctc ctcgcggaac agcgcgccct
     4261 tctcctcgga cagcaggtcg gcgcacacgc ccaccgcgcg cgacgcgtcg ggcacgtaga
     4321 ccaccgggcc ctcgtagtgc ggcgcgatct tgacggccgt gtgcacgcgg ctggtggtgg
     4381 cgccgccgat cagcagcggg atcttgagcg cgcggaagtc cggatcgcgc tgcagctcgg
     4441 aggcgacgta ctgcatctcc tccaggctgg gcgtgatcag gccggacagc ccgatgatgt
     4501 cggcgccttc gaccttggcg cgatcgagga tgtccttgca ggacaccatc acgcccatgt
     4561 tcaccacctc gaagttgttg cactggagga cgacggtgac gatgttcttg ccgatgtcgt
     4621 gcacgtcgcc cttgaccgtg gcgatgacga tcttgccctt ggagcgcacg tcgtcgccgg
     4681 cggcctccat gcgcttcttc tcttcctcga tgtaggggac gaggtgcgcg acggcggact
     4741 tcatcacgcg cgccgacttg accacctggg gcaggaacat cttgcccgcg ccgaacaggt
     4801 cgccgacgac gttcatgccg gccatcagcg ggccctcgat cacgtgcagc gggcggccgc
     4861 ccttggcgga gatcgcctgc caggcctcct cggtgtcctg gacgatgaag tcggtgatgc
     4921 cctgcaccag cgcgtgcgac aggcgctcgt cgaccgtgcc ggcgcgccag gccaggcgcg
     4981 cggagtcgtc gcgcaccgcg cccttgaccg cctcggcggc atcgagcagg cgctcggtgg
     5041 gcgtcttggc ggaggccgcg tcctcgcccg gcgcgagcgt gggcttgcga ttgaggacga
     5101 cgtcctccac cagcgtgcgc agcgtcggat cgaggttgtc gtacacgccg atcatgccgg
     5161 cgttgacgat gcccatgtcc atgcccgcct ggatggcgtg gtacaggaac acggtgtgga
     5221 tcgcctcgcg cacgggctcg ttgccgcgga acgagaagct cacgttcgac acgccgcccg
     5281 acaccttggc gcccggcagg ttctgcttga tccagcgcgt ggcctcgatg aagtcgacgg
     5341 cgtagttgtc gtgctcctcg atgcccgtgg cgatcgcgaa gatgttcggg tcgaagatga
     5401 tgtcctcggg cgggaagccc acctcgtcga ccagcacgcg gtacgcgcgc tcgcagatct
     5461 cgatcttgcg ctggtaggtg tcggcctggc cctgctcgtc gaacgccatc acgacggcgg
     5521 ccgcgccata gcggcggatc aggcgcgcct ggcgcttgaa ctcgtcgacg ccttccttca
     5581 tcgagatcga gttgacgatg cccttgccct gcaggcactt gaggccggcc tcgatcacgc
     5641 tccacttgga cgagtccacc atcaccggca cgcgcgcgat ctcgggctcg ctggccacca
     5701 ggttcaggaa gcgcaccatg gccgcctggc tgtccagcat cgcctcgtcc atgttgacgt
     5761 cgatgacctg ggcgccgttc tcgacctgct ggcgcgcgac ggccagcgcc tgctcgaact
     5821 cgccggccag gatcatgcgg gcgaaggcct tggagccggt gacgttcgtg cgctcgccga
     5881 tgttgacgaa caggtggccg gcgtccagcg tcacgggctc cagcccggac agcttcatcg
     5941 ggggcgcgat gcgggtctgc gcgccggcgg cggcgttttc tgcgggggac atgtgccaaa
     6001 ttgtaggggc cggcactcaa gcagaccctt cgcaaggccg atatgccggt tctgacagtc
     6061 tctttggcga gtccctggtt cctccatgaa ggtcgttccc ctgccctccg aaggtttgcg
     6121 catcggccgt ccgctcccgt tcacggtgcg cgacggcttc gga
//
LOCUS       DATCRO010000175         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_890825, whole genome shotgun sequence.
ACCESSION   DATCRO010000175 DATCRO010000000
VERSION     DATCRO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890825"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VIN75_04735"
     CDS             <1..175
                     /locus_tag="VIN75_04735"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY8973502.1"
                     /translation="FTRAGIDGTVEVTDTTFKLEATLGFLFSSFSEMIEQKITRNLDA
                     MLESSPRGGTRLA"
     gene            complement(188..925)
                     /locus_tag="VIN75_04740"
     CDS             complement(188..925)
                     /locus_tag="VIN75_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973503.1"
                     /translation="MTTSFDFSGRRALVTGASSGIGRAVAVRLAQAGAQVTAIGRNAA
                     ALAALHAEAGCTPLVLDVADAPALERALAPLPAFDLVVNCAGIALLEPAAEMQADSFD
                     AVMAVNARAAALVASRCGRAMIAAGVRGSIVNVSSQAALVALDAHLCYCASKAAMDAV
                     TRSLCLEFGPHGIRVNSVNPTVTLTPMAEQAWSDPAKSAAALKNIPLGRFAQVEEVVA
                     PVLFLLGDGASMVSGAALPVDGGYTSV"
     gene            1075..1575
                     /locus_tag="VIN75_04745"
                     /pseudo
     CDS             1075..1575
                     /locus_tag="VIN75_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            1572..>2011
                     /locus_tag="VIN75_04750"
     CDS             1572..>2011
                     /locus_tag="VIN75_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8973504.1"
                     /translation="MTTSFDFSGRRALGTGASSGIGRAVAVRLAQAGAQVTAIGRNAA
                     ALAALHTEAGCTPLVLDVADARALERALAPLPAFDLVVNCAGIALLEPAAEMQAESFD
                     AVMAVNARAAALVASRCGKAMIAAGVRGSIVNVSSQAALVALDA"
BASE COUNT          286 a          730 c          719 g          276 t
ORIGIN      
        1 cttcacgcgc gccggcatcg acggcaccgt cgaggtcacc gacaccacct tcaagctcga
       61 agcgacgctg ggcttcctgt tcagcagctt cagcgaaatg atcgagcaga agatcacgcg
      121 caacctcgac gcgatgctcg aatcgtctcc ccggggcggc acccgcctcg cctgaggccg
      181 cgccggctca gacgctggtg tagccgccgt ccaccggcag cgccgcgccg ctcaccatcg
      241 aagcgccatc gcccagcagg aagaggacgg gcgccaccac ttcctccacc tgcgcgaagc
      301 ggcccagcgg aatgttcttc aatgcggccg cgctcttcgc cggatcggac caggcctgct
      361 cggccatcgg cgtgagcgtg acggtcgggt tcacgctgtt cacgcggatg ccgtgcggcc
      421 cgaactccag gcacagcgat cgcgtgacgg cgtccatcgc ggccttagag gcgcagtagc
      481 aaaggtgcgc gtcgagcgcc accagcgcgg cctggctcga cacgttgacg atgctgccgc
      541 gcacgccggc cgcgatcatg gccctgccgc agcgcgaggc caccagtgcc gccgcgcgcg
      601 cgttgacggc catcaccgcg tcgaagctgt cggcctgcat ctcggcggcc ggttcgagca
      661 gcgcgatgcc ggcgcagttc accacgaggt cgaaggcggg aagcggcgcg agcgcgcgtt
      721 ccagcgctgg cgcgtcggcc acgtcgagca ccagcggcgt gcagccggct tcggcgtgca
      781 gcgctgccag cgccgcggcg ttgcggccga tggccgtcac ttgcgcgccg gcctgcgcga
      841 gccgcactgc caccgcgcgg ccgatgccgc tgctcgcgcc ggtcacgagg gcgcggcgtc
      901 cggagaagtc gaaggaggtc gtcatgtcag ccgctgcatc gcatcgcgca gcgcggggta
      961 gagcgatttg tagatctcga agcgctcgcg gtacacggcc tgcgcccggg gctcgggccg
     1021 cgcgcgttcc accagcgtga cccagccgcg ttcggcggtc gccgcagcga ccagtccggc
     1081 gccgagggcc tggtgttcct gccctacctc atgggcgagc gcagcccgat ctggaacgca
     1141 caggccaagg gcgcgttcat cgggctttcg ctcgcgcaca gccgcgcgca tctctaccgc
     1201 gcggtgctcg aaggcgtgag cttcgcgctg cagcacaaca tcgaggccgg gcggcgcagc
     1261 ggccagccgc tggacgagcg cctgatcgtg gtcggcggcg ccgcgcactc cgatctctgg
     1321 atgcagatcg tggccgacgt caccggctac ccggtgttca ccatcgagca ggaagtggaa
     1381 gcggcactgg gcgcggccat gctcgcggcg ctcggcgccg ggctggtcga tgcggccacc
     1441 gccgaacgcg gctgggtcac gctggtggag cgggcgcggc ccgagccccg cgcgcaggcc
     1501 gcgtaccgcg agcgcttcga aatctacaaa tcgctctacc ccgcgctgcg cgatgcgatg
     1561 cagcggctga aatgaccact tccttcgatt tctccgggcg tcgcgccctc gggaccggcg
     1621 cgagcagcgg catcggccgc gcggtggcgg tgcggctcgc gcaggccggc gcgcaagtga
     1681 cggccatcgg ccgcaacgcc gcggcgctgg cagcgctgca caccgaagcc ggctgcacgc
     1741 cgctggtgct cgacgtggcc gacgcgcgag cgctggaacg cgcgctcgcg ccgcttcccg
     1801 ccttcgacct ggtggtgaat tgcgccggca tcgcgctgct cgaaccggcc gccgagatgc
     1861 aggccgagag cttcgatgcg gtgatggccg tcaacgcgcg cgcggcggca ttggtggcct
     1921 cgcgctgcgg caaggccatg atcgcggccg gcgtgcgcgg cagcatcgtc aacgtgtcga
     1981 gccaggctgc gctggtggcg ctcgacgcgc a
//
LOCUS       DATCRO010000176         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_35488, whole genome shotgun sequence.
ACCESSION   DATCRO010000176 DATCRO010000000
VERSION     DATCRO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35488"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VIN75_04755"
     CDS             complement(<1..1227)
                     /locus_tag="VIN75_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8973505.1"
                     /translation="MSGAKDNTADRTRTASRQRSGVPAEREEHWLAEGFARHAARWAT
                     SAGANAAGVAAIARAAAALSRAVSEGHVCLVLASLAAGSGDDGDAPSRLDVAGWRAQL
                     LASGIVGTPDAPGVLPLILDDEDRLYLHRYFDFERRLARRLVQARHAGAREATDAPEL
                     RAQLAELFAANAARLGGAADWQQLAAALALRGRLTVISGGPGTGKTTTVVNLLACLLA
                     LDPDCRIALAAPTGKAAARMTDAIRQRAQHLPATLRDRMPAESSTVHRLLGVTPDGFM
                     HDSGNPLAIDALVVDEASMLDLALATRLLDAVPPAARIILLGDKDQLAAVESGAVFAE
                     LSVDPTLTADCVRDLAALTGTPAAAIAPPPPARPSALQDCAVWFTQTYRFSVDSGVGR
                     LAHDVNAGRARAAADWL"
     gene            complement(1224..>3720)
                     /locus_tag="VIN75_04760"
     CDS             complement(1224..>3720)
                     /locus_tag="VIN75_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8973506.1"
                     /translation="FRTIYTAPTDDTLAAAPLFFVGDPKQAIYSFRNADLHTYLEAGT
                     HAVARYSLGENQRSTEPLIRALNALFTANARAFMMDGLDYQTVAFGKKPREAFIDTSA
                     PRAPLQVWTLPPEGDGEPMFLREATEAVVAATADEIARLLAGAQRGEVTLGEGAKARP
                     LAAGDIAVLVRSNRQGSQIRQALTARGVGSVELSQASVFRSSDAEELDRVLTAILEPA
                     RERTLKAALSTGLMGDDAAAILALAADESALLAAVQRFAGWREVWLQRGVGFMLRQWL
                     AAEQVAGRLLARPDGERRLTNLLHLVECLHEAGERHAAPDTLLRWFQQQRVAPAADDA
                     TQLRLESDQNLVQIVTIHKSKGLEYPVVFCPFLWSAARGGRPDGLTGHAYHDEHGGTV
                     IDYGHDFRDEAHQKAVKLRQKLEESAETLRLVYVALTRAVHRCVLVAGCYRSKVGRNV
                     SSTASTRSLLNWLVAGGELSPEDWFEHKNGAPAIESAWQALARSAGGAIAVEPLPRGA
                     LVALQPSRPSPDTLAALPAPSSLPMGWRIGSFSAIAHGAAHDRSGVDHDLRAADVAAP
                     RDDDTSFHADDDDPLAFPRGPAAGEAIHLAFENIDFTDAAGWPQAIDQALHLLRAMGE
                     PEAPGDAALRARMLARLIEGTLTTALPLGTAQPLRLAELPRSRRLVELEFHLPSHRFD
                     APALNALLEAHGYAMPRLSFATLRGFLKGFIDLVFEHEGRFFILDWKSNHLGDSPARY
                     GQEAMAAAMRDQGYHLQYLLYLVALHRYLRLRLPDYDPGRHLGGAVYLFVRGVRPGWH
                     DATGKPTGVFFHRPSTATLQALSALFDETEATA"
BASE COUNT          477 a         1314 c         1444 g          485 t
ORIGIN      
        1 cagccagtcg gccgcggcgc gcgcgcggcc ggcgttgacg tcgtgcgcca gccggccgac
       61 gcccgagtcg accgagaagc ggtaggtctg cgtgaaccag accgcgcagt cctgcagcgc
      121 gctgggccgc gcgggcggcg gcggcgcgat ggcggcggcg ggcgtgccgg tcagcgcggc
      181 caggtcgcgc acgcagtccg cggtgagcgt cgggtcgacg ctgagctcgg cgaacaccgc
      241 accggattcc accgcggcca gctgatcctt gtcgccgagc aggatgatgc gcgcggcggg
      301 cggcacggcg tccagcaggc gggtggccag cgcgagatcg agcatcgagg cttcgtcgac
      361 gacgagcgcg tcgatggcca gcgggttgcc cgaatcgtgc atgaagccgt ccggcgtcac
      421 gcccagcagg cggtgcacgg tggaggactc cgcgggcatc cggtcgcgca gcgtcgcggg
      481 caggtgttgc gcgcgctggc ggatcgcgtc cgtcatgcgc gccgcggcct tgccggtcgg
      541 cgcggccagt gcgatgcggc agtcgggatc cagcgcgagc aggcaggcga gcaggttgac
      601 gacggtggtc gtcttgccgg tgccggggcc gccgctgatc accgtcaagc gcccgcgcag
      661 cgccagcgcg gcggcgagct gctgccagtc ggccgcgccg cccaggcgcg cggcgttggc
      721 ggcgaacagc tcggccagct gtgcgcgcag ctcgggcgca tcggtcgcct cgcgagcgcc
      781 cgcatgacgc gcctgcacga gccggcgcgc gaggcggcgc tcgaagtcga agtagcggtg
      841 caggtacagg cggtcctcgt cgtcgaggat cagcgggagc acgcccggcg catccggcgt
      901 gcccacgatg cccgaggcca gcagctgggc gcgccaaccc gcgacgtcca ggcgcgacgg
      961 tgcgtcaccg tcgtcgccgc tgcctgccgc gagcgacgcc agcaccaggc acacgtgtcc
     1021 ctcgctgacc gcccggctca gcgccgcggc cgcacgtgcg atcgccgcga cgcccgccgc
     1081 attcgcgccc gcggacgtgg cccagcgcgc ggcatgccgt gcgaagccct cggcgagcca
     1141 gtgctcctcg cgctcggccg gaacgccgct gcgttgccgg gacgccgtgc gcgtgcgatc
     1201 cgcggtgttg tccttggcgc cgctcatgcg gtggcctccg tctcgtcgaa cagcgccgac
     1261 agcgcctgga gggtggccgt ggacggccgg tggaagaaca cgcccgtggg cttgcccgtg
     1321 gcgtcgtgcc agccaggccg cacgccgcgc acgaacaggt agacggcgcc gccgaggtgg
     1381 cggcccgggt cgtagtcggg cagccgcagc cgcaggtagc ggtgcagagc gacgaggtac
     1441 agcaggtact gcaggtggta gccctggtcg cgcattgccg ccgccatggc ctcctggccg
     1501 tagcgcgccg gcgagtcgcc caggtggttg gacttccagt cgaggatgaa gaaacgccct
     1561 tcatgctcga acacgaggtc gatgaagccc ttgaggaacc cgcgcagcgt cgcgaacgac
     1621 agccgcggca tcgcgtagcc gtgcgcctcg agcagcgcgt tgagcgccgg cgcgtcgaag
     1681 cggtgcgacg gcaggtggaa ctcgagctcg acgaggcgcc gcgatcgcgg cagctcggcc
     1741 agccgcaacg gctgcgccgt gcccagcggg agcgcggtcg tgagcgtgcc ttcgatgagg
     1801 cgcgccagca tgcgggcgcg cagagcggcg tcgcccgggg cctcgggctc gcccatggcg
     1861 cgcagcaggt gcagcgcctg gtcgatcgcc tgcggccagc cggcggcatc ggtgaagtcg
     1921 atgttctcga acgcgaggtg gatggcctcg ccggcggccg ggccgcgcgg gaaggcgagc
     1981 gggtcgtcgt cgtccgcgtg gaagctcgtg tcgtcgtccc gcggcgcggc gacgtcggcc
     2041 gcgcgcaggt cgtggtcgac gccggagcgg tcgtgcgcgg cgccgtgcgc gatggcactg
     2101 aagctgccga tgcgccagcc catcggcagc gaggacgggg caggcagggc ggcgagcgtg
     2161 tcgggcgacg gacgcgacgg ctgcagcgcc acgagcgcgc cgcggggcag cggctcgacc
     2221 gcgatcgcgc cgccggcgct ccgggccagc gcctgccacg ccgattcgat ggcgggcgcg
     2281 ccgttcttgt gctcgaacca gtcctcgggc gacagctccc cgccggccac cagccagttc
     2341 agcaggctgc gcgtgctggc agtgctgctg acgttgcgac cgaccttgct gcggtagcac
     2401 ccggccacca gcacgcagcg gtgcaccgcg cgcgtcaacg cgacgtagac caggcgcagc
     2461 gtctcggcgg actcctcgag cttctggcgc agcttgacgg ccttctggtg cgcctcgtcg
     2521 cggaagtcgt ggccgtagtc gatgacggtg ccgccgtgct cgtcgtggta cgcgtggccc
     2581 gtgagcccgt cgggccggcc gccgcgcgcg gcgctccaga ggaacgggca gaacaccacc
     2641 gggtactcca ggcccttgga cttgtggatc gtgacgatct gcaccaggtt ctggtcggac
     2701 tccagccgca gctgcgtcgc gtcgtccgcc gcgggcgcga cgcgctgctg ctggaaccag
     2761 cgcagcaggg tgtcgggggc ggcgtgacgc tcgcccgcct cgtgcaggca ttcgaccagg
     2821 tgcagcaggt tggtcaggcg ccgttcgccg tcggggcgcg ccagcaggcg cccggccacc
     2881 tgttccgcgg ccagccactg gcgcagcatg aagccgacgc cgcgctgcag ccagacctca
     2941 cgccagccgg cgaagcgctg cacggcggcc agcagcgccg actcgtctgc ggccagcgcc
     3001 aggatggccg cggcgtcatc gcccatcagg ccggtggaca gcgccgcctt gagcgtgcgt
     3061 tcgcgcgccg gctcgaggat ggcggtgagg acgcgatcga gttcctcggc gtcgctgctg
     3121 cggaacacgc tggcctgcga cagctcgacg ctgcccacgc cgcgcgcggt cagcgcctgc
     3181 cggatctgag agccctggcg gttgctgcga acgagcaccg cgatgtcgcc ggccgcgagc
     3241 gggcgcgcct tcgcgccttc gccgagcgtg acctcgccgc gctgggcccc cgccagcagg
     3301 cgcgcgatct cgtcggccgt ggcggcgacg acggcctcgg tcgcctcgcg caggaacatc
     3361 ggctcgccgt cgccctccgg cggcagcgtc cacacctgga gcggcgcgcg cggggcgctg
     3421 gtgtcgatga acgcctcgcg cggcttcttg ccgaacgcga ccgtctggta gtcgagcccg
     3481 tccatcatga aggcgcgcgc attggccgtg aacagggcgt tgagcgcgcg gatcagcggt
     3541 tcggtggagc gctggttctc gccgagcgag tagcgcgcca ccgcgtgcgt gccggcctcc
     3601 aggtaggtgt gcaggtcggc gttgcggaag ctgtagatcg cctgcttcgg gtcgccgacg
     3661 aagaacagcg gcgcggcggc gagcgtgtcg tccgtcgggg cggtgtagat cgtcctgaag
//
LOCUS       DATCRO010000177         7084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_463219, whole genome shotgun sequence.
ACCESSION   DATCRO010000177 DATCRO010000000
VERSION     DATCRO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463219"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1470)
                     /locus_tag="VIN75_04765"
     CDS             complement(187..1470)
                     /locus_tag="VIN75_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEY8973507.1"
                     /translation="MSGARPPETAAVIGAGPAGLMAADALQAAGVQVHVFDAMPSAGR
                     KFLLAGIGGLNLTHSEAMPGFLARYAERAPALQPLLDDFGPDATRAWAGELGIETFVG
                     TSGRVFPKDMKAAPLLRAWLHRLRAGSDARPPVQFHMRHRWLGWSDDGAALRIASLDG
                     ESTFAADATVLALGGASWPKLGSDGAWVPLLAGRGIDVAPLRPSNCGFDVAPTGPDRT
                     GWSAHLRERFAGEPVKPVAITAADASGVAHRQQGEFVVTRTGVEGSLVYAFSAGLREQ
                     IAARGEATLTLDLLPQHTPEQVLAETRRPRGARSLSTHLKSRLHLQGVKTSLLHELLT
                     AAQMNDPDALAGFIKALPLRLAAARPIAEAISSAGGVRFEALDDALMARASPGLFVAG
                     EMLDWEAPTGGYLLTACLATGQAAGRGAAAWLRGR"
     gene            complement(1467..2810)
                     /locus_tag="VIN75_04770"
     CDS             complement(1467..2810)
                     /locus_tag="VIN75_04770"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8973508.1"
                     /translation="MRQPPPAGLAADFAAWGISDVECLFPDVSGYPRGKLMPASAFAA
                     GAELRIAQAIPMQCVTGDYSYDPIFPDQDPDVALVPDYATVRPVPWAGVPRALAIHDC
                     FELNGEPCRFAPRSILRDVMARYEKRGLRAVVAPEIEFYLTAPNDDPAQPLRAPAMRG
                     GRAEVGQSAFSLNTLNEHAVFWTEFRAALDTLGVQADTWIHEVGLTQFEINLLHDDPL
                     AVADQAFLFKYAAKEVALKHGLNAVFMAKPIAGQPGSSMHLHQSVVDADGRNVFSNAD
                     GGESLLFRQFIAGLQANLPSLMLLFAPFVNSYRRFVRQSQAPVNFHWGYDNRTAGLRI
                     PRSGPAARRVENRVAGADANPYLVIAATLAAGLDGIERALPPSDPMDDSAYEQAHMLP
                     RSLITAIESFAGDGVATRLFGERFVRGYASTKEAEYESWLAEIGAWERRFLVPQA"
     gene            3039..4253
                     /locus_tag="VIN75_04775"
     CDS             3039..4253
                     /locus_tag="VIN75_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8973509.1"
                     /translation="MPLERPPFDTIALVLQGGGALGAYQAGVMEGLADAGIEPDWVAG
                     ISIGAINAALIAGNAPANRIDRLREFWTTVSQPAVWPDALSFLDAFVEPADGWRRQFW
                     NNLSAWRALTEGQRGFFTPRPATAFVGPQPPDAASFYDTAPLKATLERLVDFDRINAG
                     QMRLTVSAVDVETGNFEAFDNQHGRWRKRIRAEHIMASGALPPGFPAVAIEEPGPLEP
                     SRASGAPRRVRHFWDGGLVSNTPLSQVLDDHPRRDTLAFQVDLWSARGKLPTNVIDAQ
                     ERVKDIQYSSRTRAITNMQASEQRQRRMLRELLELIPPEERARHPAARAAAQQATARS
                     VSVIQLIYQDKEWDGLSKDYEFGAVTMRSHWDSGLADIRRTLEHPHWLERPQGDDPFV
                     THDIHRQRRSAD"
     gene            4516..5730
                     /locus_tag="VIN75_04780"
     CDS             4516..5730
                     /locus_tag="VIN75_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY8973510.1"
                     /translation="MSSIKILVAQGGGPTAVINQSLVGVVLEARRFPQVERVYGARHG
                     VRGIVDEDLVDLTQETLANLEQVAATPASALGSTRDKPDLAYCQRILATLQAHGIGHF
                     FYIGGNDSSDTVRIVAEEARKAGHPLRCVHIPKTIDNDLMGNDHTPGFPSAARFVAQA
                     FAGANLDNAALPGVYVGVIMGRHAGFLTAAAALARRFPGDGPHLVYLPERTFKVDSFL
                     ADVKATYEKHGRCVIAVSEGIHDETGATIAEKLARNVERDAHGNVQLSGTGALADLLC
                     DQIKDKLRIKRVRGDTFGYLQRSFLGCVSDVDQREAREVAEKAVQLAMWGDRDGSVAI
                     RRTGDYAVDYVLQPLEAVAAKTRTMEDALIAPAGNDVTPAFFDYLRPLLGADMPVAGR
                     LRAPAVAKVLKR"
     gene            complement(5761..>7084)
                     /locus_tag="VIN75_04785"
     CDS             complement(5761..>7084)
                     /locus_tag="VIN75_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973511.1"
                     /translation="ADGVKLLLRMRGRAVDPKRRQETGTIWVVEDITDRRRAELELAD
                     AQRDAEAASRAKSSFLATMSHEIRTPLSGVLGLARLLQDPALSAERRKSYLTHLVDAA
                     ELLNGIVSDVLDLSKIEAGHLQVEHIPFDLHAVVWSAFRTFSPIGQERGLEMSCHVAL
                     DTPREVLGDPVRVRQILSNYLSNALKFTQHGRIHVHLYRRSHEVARIEVSDTGIGVSP
                     ELRTTLFQPFTQADSSTTRRFGGSGLGLSICRELATLMGGDVGLDSDGHSGTCAWVDL
                     PLVTASEEDFVTVASPLDDAAPKQPLQGMCVLLAEDNAVNRLIVGTMLVRLGADVIEA
                     TNGSEAIEQATRAPDDVHAILMDLHMPEVDGLEATRRLRAQPATAHLPIIALTAAVLD
                     AERAQAHAAGMNGFVSKPTGEGELLRALWAYVPGVEGPASAFMRFDVD"
BASE COUNT         1009 a         2550 c         2560 g          965 t
ORIGIN      
        1 gcatctcgcc ggccacgaac aggcctgggg acgcgcgcgc catcagcagc gggccgggcg
       61 ccttgatctc ggcgctggcc ggggtgtcga cgtcgaacac gggttcggga cggggacggc
      121 gggacgggga acgcgcgggc atgcggggaa tggatctgtc ggaagcgcca atggtagccg
      181 gcggcttcag cggccgcgca gccacgccgc ggcgccgcgg ccggccgcct gtccggtggc
      241 caggcacgcg gtgagcaggt agccgccggt gggcgcctcc cagtcgagca tctcgccggc
      301 cacgaacagg cctggggacg cgcgcgccat cagcgcatcg tccagcgcct cgaagcgcac
      361 gccgccggcg ctcgaaatgg cctcggcgat cgggcgcgcc gcggccagcc gcagcggcag
      421 tgccttgatg aagccggcga gcgcgtcggg atcgttcatc tgcgcggccg tcagcagttc
      481 atgcagcagg ctcgtcttga cgccctgcag gtgcagccgg ctcttcagat gggtcgacag
      541 gctgcgcgcg ccgcgcggcc ggcgcgtctc ggccagtacc tgctcgggcg tgtgctgcgg
      601 cagcaggtcg agcgtgagcg tggcctcgcc gcgcgcggcg atctgctcgc gcaggccggc
      661 ggagaacgcg tagacgaggc tgccctcgac gcccgtccgg gtgacgacga actcgccctg
      721 ctgccggtgc gcgacgccgc tcgcgtcagc cgcggtgatc gccaccggct tgaccggctc
      781 gcccgcgaag cgctcgcgca ggtgcgcgct ccagcccgtg cgatcggggc cggtgggcgc
      841 gacgtcgaag ccgcagttgc tcgggcgcag cggcgcgacg tcgatgccgc gtccggccag
      901 cagcggcacc cacgcgccgt cggaaccgag cttgggccag ctcgcgccgc cgagcgcgag
      961 cacggtggca tcggccgcga aggtcgactc gccgtcgagg ctcgcgatgc gcagcgcggc
     1021 gccgtcgtcg ctccagccga gccagcggtg gcgcatgtgg aactgcacgg gcggccgcgc
     1081 atcgctgccg gcgcgcaggc ggtgcagcca cgcgcgcagc agcggcgcgg ccttcatgtc
     1141 cttcgggaac acgcggcccg aggtgccgac gaaggtctcg atgcccagtt cgcccgccca
     1201 ggcacgcgtg gcgtcgggcc cgaagtcgtc gagcaagggc tgcaacgccg gcgcgcgttc
     1261 cgcgtagcgc gcgagaaagc cgggcatcgc ctcggagtgc gtcaggttca ggccgccgat
     1321 gcccgccagc aggaacttgc ggccggccga cggcatcgcg tcgaaaacgt gcacctgcac
     1381 gccggcggcc tgcagggcgt ctgcagccat caggcccgcg gggccggcgc cgatgacggc
     1441 ggcggtttcg ggcggccgtg cgccgctcat gcctgcggca ccaggaaacg ccgctcccag
     1501 gcgccgatct cggcgagcca gctctcgtac tccgcttcct tggtggatgc atagccgcgc
     1561 acgaagcgtt cgccgaacag gcgggtggcc acgccgtcgc ccgcgaacga ctcgatggcg
     1621 gtgatcagcg agcgcggcag catgtgtgcc tgctcatagg cgctgtcgtc catcggatcg
     1681 ctgggcggca gcgcgcgctc gatgccgtcg aggccggcgg ccagcgtcgc ggcgatcacg
     1741 aggtacgggt tcgcgtccgc cccggccacg cggttctcca cgcggcgcgc ggccgggccg
     1801 ctgcgcggga tgcgcaggcc ggcggtgcgg ttgtcgtagc cccagtggaa gttcaccggc
     1861 gcctggctct gcctgacgaa gcgccggtac gagttgacga agggcgcgaa cagcagcatc
     1921 aggctgggca ggttggcctg caggccggcg atgaactggc ggaacaacag gctctcgccg
     1981 ccgtcggcgt tgctgaagac gttgcggccg tcggcgtcca cgacgctctg gtgcaggtgc
     2041 atcgagctgc ccggctggcc ggcgatcggc ttggccatga acaccgcgtt caggccgtgc
     2101 ttgagtgcga cttccttggc cgcatacttg aacaggaagg cctggtcggc caccgcgagc
     2161 gggtcgtcgt gcagcaggtt gatctcgaac tgcgtcaggc cgacctcgtg gatccacgtg
     2221 tcggcctgga cccccagcgt gtccagcgcg gcgcggaatt ccgtccagaa cacggcgtgc
     2281 tcgttgagcg tgttcaggct gaaggccgac tggccgacct cggcgcggcc gccgcgcatc
     2341 gccggcgcgc gcagcggctg ggccgggtcg tcgttgggcg cggtcagata gaactcgatc
     2401 tcgggcgcga cgacggcgcg caggccgcgc ttctcgtagc gcgccatcac gtccctcagg
     2461 atgctgcgcg gcgcgaaccg gcagggctcg ccgttcaact cgaagcagtc gtggatcgcg
     2521 agcgcgcgcg gcacgccggc ccacggcacg ggacgcacgg tggcgtagtc gggcacgagc
     2581 gcgacgtcgg gatcctggtc gggaaagatc gggtcgtacg agtagtcgcc cgtcacgcat
     2641 tgcatcggga tcgcctgcgc gatccgcagc tcggcgccgg ccgcgaaggc cgaggccggc
     2701 atcagcttgc cgcggggata gccggatacg tcgggaaaca ggcactcgac gtccgagatg
     2761 ccccaggccg cgaaatcggc cgccaggccg gcggggggtg gttggcgcat cccgccatta
     2821 gaccagcgag gtcggggtca ggtcttgaaa gttgcggggt caggtcttga atcgtgcaac
     2881 tccacgcctg ccagcacttc gccgtggcag tgactgcgcg gcgacgttcg aatcccgact
     2941 cgactgcgca acattcaaga cctgaccccg caactttcaa gacctgaccc cgcgatgccg
     3001 ataagctccg ggctcccgtc catcacttgc cgcacgccat gccgctcgaa cgtccgccct
     3061 tcgataccat cgccctcgtg ctgcagggcg gcggcgcgct gggcgcctac caggccgggg
     3121 tgatggaggg gctggcggac gcgggcatcg agcccgactg ggtcgccggc atctcgatcg
     3181 gcgcgatcaa cgccgcgctg atcgcaggca acgcgccggc gaaccgcatc gaccggctgc
     3241 gcgagttctg gacgacggtg tcgcaaccgg ccgtctggcc ggacgcgctg tcgttcctcg
     3301 acgccttcgt cgagcccgcc gacggctggc ggcggcagtt ctggaacaac ctgagcgcct
     3361 ggcgcgcgct caccgagggc cagcgcggct tcttcacgcc gcggccggcg accgcgttcg
     3421 tcggcccgca gccgcccgac gcggccagct tctacgacac cgcgccgctc aaggcgacgc
     3481 tggaacgcct ggtcgacttc gaccgcatca acgccggcca gatgcggctg acggtctcgg
     3541 ccgtggacgt ggagaccggc aacttcgagg cgttcgacaa ccagcacggc cgctggcgca
     3601 agcgcatccg cgccgagcac atcatggcct cgggcgcgct gccgccgggc tttccggcgg
     3661 tcgccatcga ggagcccggt ccgctggagc cctcgcgcgc gagcggcgcg ccgcggcgcg
     3721 tgcgccactt ctgggacggc ggcctcgtct ccaacacgcc gctgtcgcag gtgctggacg
     3781 accacccgcg gcgcgacacc ctggccttcc aggtcgacct gtggagcgcg cgcggcaagc
     3841 tgcccaccaa cgtgatcgac gcgcaggaac gcgtcaagga catccagtac tccagccgga
     3901 cccgagccat caccaacatg caggcgagcg agcagcgcca gcgccgcatg ctgcgcgagc
     3961 tgctggaact gatcccgccc gaggaacgcg cccgccaccc cgccgctcgc gccgccgcgc
     4021 agcaggccac ggcgcgcagc gtcagcgtca tccagctgat ctaccaggac aaggagtggg
     4081 acggcctttc caaggactac gagttcggcg ccgtcacgat gcgcagccac tgggacagcg
     4141 gcctggccga catccgccgc acgctggagc atccgcactg gctggagcgg ccgcagggcg
     4201 atgatccgtt cgtgacgcac gacatccacc ggcagcggcg gtcggcggac tgacgccgcc
     4261 gggccggtga ggtgcgcccg ggtctgtcga tgacgacctg gcgcgccggc acgaggcgcg
     4321 gtctccggtc gccatccccg tcatcctgcg cgaggcatgg atgcgccgtg cggcgaaggg
     4381 acgctttgcc ggccgggatc gatcctgcga cagcgcgcag gatgacacct ggttggcgcc
     4441 gggcggcaac agtgcctcgc gcgaagccgg tcggtcgtaa gatcgagccc cgtccaacgt
     4501 ctggagtgcc tgcccatgag ttcgatcaag atcctcgtcg cgcaaggcgg cggccccacc
     4561 gcggtgatca accagtcgct ggtgggcgtc gtgctcgagg cgcggcgctt cccgcaggtc
     4621 gagcgcgtgt acggcgcgcg gcacggcgtg cgtggcatcg tcgacgagga cctggtcgac
     4681 ctgacccagg agacgctggc caacctcgag caggtggccg ccacgccggc ctcggccctc
     4741 ggttccacgc gcgacaagcc cgacctcgcc tactgccagc gcatcctcgc gaccctgcag
     4801 gcgcacggca tcggccactt cttctacatc ggcggcaacg actcgtccga caccgtgcgc
     4861 atcgtcgccg aggaggcgcg caaggcgggc cacccgctgc gctgcgtgca cattcccaag
     4921 acgatcgaca acgacctgat gggcaacgac cacacgcccg gcttcccgtc ggccgcgcgc
     4981 ttcgtggccc aggccttcgc gggcgcgaac ctcgacaacg ccgcgctgcc gggcgtctac
     5041 gtcggcgtca tcatgggtcg gcacgccggc ttcctgacgg ccgcggcggc gctcgcccgg
     5101 cgcttccccg gcgacggccc gcacctggtc tacctgccgg agcgcacgtt caaggtcgac
     5161 agcttcctgg ccgacgtcaa ggccacgtac gagaagcacg gccgctgcgt gatcgccgtc
     5221 tcggaaggca tccacgacga gaccggcgcg accatcgccg agaagctcgc caggaacgtc
     5281 gagcgcgacg cgcacggcaa cgtgcagctg tcgggcaccg gcgcgctggc cgacctgctg
     5341 tgcgaccaga tcaaggacaa gctcaggatc aagcgcgtgc gcggcgacac cttcggctac
     5401 ctgcagcgct cgttcctcgg ctgcgtgtcc gacgtcgacc agcgcgaggc gcgcgaggtc
     5461 gccgagaagg ccgtgcagct ggcgatgtgg ggcgaccgcg acggctccgt ggccatccgc
     5521 cgcaccggcg actacgccgt cgactacgtg ctgcagccgc tggaggccgt ggccgccaag
     5581 acgcgcacga tggaggacgc gctgatcgcg cccgcgggca acgacgtcac gcccgccttc
     5641 ttcgactacc tgcggccgct gctgggcgcc gacatgcccg tggcgggccg cctgcgcgcg
     5701 ccggcggtgg ccaaggtgct caagcgctga cgccgcgcga ccgcgtggcc ggccggccgc
     5761 tcaatcgacg tcgaagcgca tgaaggcgct cgccggcccc tccacgcccg gcacgtaggc
     5821 ccacagcgcc cgcagcagct cgccctcgcc ggtgggcttg gagacgaacc cgttcatgcc
     5881 cgccgcatgc gcctgcgcgc gctcggcgtc cagcacggcg gcggtcagcg cgatgatggg
     5941 aaggtgcgcg gtggcgggct gcgcgcgcag gcggcgcgtg gcctccaggc cgtcgacctc
     6001 gggcatgtgc aggtccatca ggatcgcgtg cacgtcgtcg ggcgcgcggg tggcctgctc
     6061 gatcgcctcg ctgccgttgg tggcctcgat cacgtcggcg ccaaggcgca ccagcatcgt
     6121 gccgacgatc agccggttga ccgcgttgtc ctcggccagc agcacgcaca tgccctgcag
     6181 cggctgcttg ggcgcggcgt cgtccagcgg gctcgcgacg gtgacgaagt cctcctcgct
     6241 ggccgtcacc agcggcaggt cgacccacgc gcaggtgccg ctgtggccat cgctgtccag
     6301 gccgacgtcg ccgcccatca gcgtggccag ctcgcggcag atcgacaggc ccaggccgct
     6361 gccgccgaag cgccgcgtgg tggagctgtc ggcctgcgtg aacggctgga acagcgtggt
     6421 gcgcagctcg gggctgacgc cgatgccggt gtcgctgacc tcgatgcgcg ccacctcgtg
     6481 cgagcgccgg tacaggtgca cgtggatgcg gccgtgctgc gtgaacttca gcgcgttgga
     6541 caggtagttc gacaggatct gccgcacccg caccgggtcg cccagcacct cgcgcggcgt
     6601 gtcgagcgcc acgtggcagc tcatctccag cccgcgctcc tggccgatcg gcgagaaggt
     6661 gcggaacgcg ctccagacga cggcgtgcag gtcgaacggg atgtgctcga cctgcaggtg
     6721 cccggcctcg atcttggaca ggtccagcac gtccgacacg atgccgttca acagttcggc
     6781 ggcatcgacc aggtgcgtga ggtaggactt gcggcgctcg gccgacagcg ccggatcctg
     6841 cagcagccgc gccagcccca gcaccccgct gagcggcgtg cggatctcat ggctcatcgt
     6901 ggccaggaag ctgctcttgg cgcggctggc cgcctcggcg tcgcgctgcg cgtcggccag
     6961 ctcgagctcg gcgcggcggc ggtcggtgat gtcctcgacc acccagatgg tgccggtctc
     7021 ctggcgccgc ttcgggtcga cggcgcggcc gcgcatgcgc agcagcagct tgacgccgtc
     7081 ggcc
//
LOCUS       DATCRO010000178         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_305568, whole genome shotgun sequence.
ACCESSION   DATCRO010000178 DATCRO010000000
VERSION     DATCRO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305568"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..319
                     /locus_tag="VIN75_04790"
     CDS             122..319
                     /locus_tag="VIN75_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973512.1"
                     /translation="MGIAFDALVQLLAAIFGIETVSLAALTGQMVLSIAYFLSMWFGF
                     VDTFDITDSRTRGAEDAAPAS"
     gene            421..1560
                     /locus_tag="VIN75_04795"
     CDS             421..1560
                     /locus_tag="VIN75_04795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8973513.1"
                     /translation="MKTTETIHKLNKLAHSHGPLPAGRGMGSGVVALVLAILCFLGVL
                     AFHFPQYLSTPELRKSYDVATIRLIMYWAMVVAGGISLYNILFARTRWLAASAFFLVA
                     ASALLGGAKVPVNNFPDHTPYIGLDWFILDLLGSSLIFVFIEKLFALRREQPVFRAEW
                     QVDMQHFIVNHMIVGFILLATNLLVHKLFGWAAKDGIQAWVRDLPYPVALFLIILVAD
                     LVQYATHRAYHEVPVLWRLHAVHHSVKSMDWLAGSRQHVVELLITRTLVLAPIFVLGF
                     DKGVIDTYIVIVGFQAVFNHANVSVRLGPLRYLIVTPNFHHWHHSQDDEAIDRNYAAH
                     FAFIDYLFGTAVKSDRKWPDQYGVVGDYVPDGFWKQVKFPFTWKG"
     gene            complement(1610..>2206)
                     /locus_tag="VIN75_04800"
     CDS             complement(1610..>2206)
                     /locus_tag="VIN75_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973514.1"
                     /translation="WLGREPVAEAAQVEAAAVRIEPLDESGFEEFFRYLGDHLTGNGR
                     DGKYFMPFPHEAAPGFPADKQESFTGGLGVAVGRPGWRRAWVARDARGRPIGHVDLRA
                     HPEPGTLHRCLLGMGVDRDHRRLGLARKLLAHATQWAVDQDLRWMDLRVLSANEAAVA
                     LYRVEGFQMQGGTPDMFRVDGQSLGYMFMAKRLGQPAA"
BASE COUNT          321 a          800 c          717 g          368 t
ORIGIN      
        1 gtacgcgccg gcgctggtgt actggggcgg acagggcgcc gcccaggcca tgttctccag
       61 catcgtggcg atctggcgca ccaaggccgc cttcctcgcc tagctggccg gctggttcgt
      121 ggtgggtatc gcgttcgacg cgctcgtgca gctgctggcg gcgatcttcg gcatcgagac
      181 ggtctcgctg gcggccctga cgggccagat ggtgctgagc atcgcctact tcctgtcgat
      241 gtggttcggc ttcgtcgaca ccttcgacat cacggactcg cgcacgcggg gcgccgaaga
      301 cgccgctccg gcttcctgac aaacccggga catcccccgg gagaggggcg acaggccgcc
      361 cgaaatcggc ttcaatccag gctcgcgccg ccgcgcgccc tggaaccgcc aagcccccgg
      421 atgaagacga ccgagacgat ccacaagctc aacaagctcg cccactcgca cggaccgctg
      481 cccgccgggc gcggcatggg ctccggcgtc gtcgccctgg tcctggccat cctctgcttc
      541 ctgggcgtgc tggcgttcca cttcccgcag tacctgtcca cgccggaact gcgcaagtcc
      601 tacgacgtcg ccacgatccg cctgatcatg tactgggcga tggtggtggc cggcggcatc
      661 tcgctgtaca acatcctgtt cgcgcgcacc cgctggctcg ccgcgtcggc gttcttcctc
      721 gtggcggcgt cggcgctgct gggcggcgcc aaggtgccgg tcaacaactt tcccgaccac
      781 acgccctaca tcgggctgga ctggttcatc ctcgacctgc tgggctcgtc gctgatcttc
      841 gtgttcatcg agaagctgtt cgcgctgcgc cgcgagcagc ccgtcttccg cgccgagtgg
      901 caggtggaca tgcagcactt catcgtcaac cacatgatcg tcgggttcat cctgctggcg
      961 accaacctgc tggtgcacaa gctgttcggc tgggctgcca aggacggcat ccaggcctgg
     1021 gtgcgcgacc tgccctaccc cgtcgcgctg ttcctgatca tcctcgtcgc cgacctcgtg
     1081 cagtacgcca cccaccgcgc gtaccacgag gtgcccgtcc tgtggcggct gcacgccgtg
     1141 caccacagcg tcaagagcat ggactggctg gccggctcgc gccagcacgt ggtcgagctg
     1201 ctgatcaccc gcacgctggt gctggcgccg atcttcgtgc tgggcttcga caagggcgtg
     1261 atagacacct acatcgtgat cgtcggcttc caggccgtgt tcaaccacgc caacgtcagc
     1321 gtgcgcctcg ggccgctgcg ctacctgatc gtcacgccca acttccatca ctggcaccac
     1381 tcgcaggacg acgaggcgat cgacaggaac tacgccgcgc acttcgcctt catcgactac
     1441 ctgttcggca ccgcggtgaa gtccgaccgc aaatggccgg accagtacgg cgtcgtgggc
     1501 gactacgtgc ccgacggctt ctggaagcag gtcaagttcc cgttcacctg gaagggttga
     1561 ggagcgcggc tgcgagtcgg gtcggggcgc cgcggcgccc cgatggcctt caggccgccg
     1621 gctgccccag cctcttggcc atgaacatgt agccgagcga ctgcccgtcg acccggaaca
     1681 tgtcgggcgt gccgccctgc atctggaagc cttcgacacg gtacagcgcg accgcggctt
     1741 cgttggccga cagcacgcgc aggtccatcc agcgcaggtc ctggtcgacg gcccactgcg
     1801 tcgcgtgcgc cagcagcttg cgcgcgaggc ccaggcggcg atggtcgcga tcgacaccca
     1861 tgcccagcag gcagcgatgg agcgtgcccg gctcggggtg cgcgcgcagg tcgacgtggc
     1921 cgatcgggcg cccgcgcgcg tcacgcgcga cccacgcgcg acgccagccc ggccggccca
     1981 cggccacgcc cagcccaccg gtgaacgact cctgcttgtc cgccgggaac ccgggggccg
     2041 cctcgtgcgg gaacggcatg aagtacttgc cgtcgcgtcc gttgccggtg aggtggtcgc
     2101 ccaggtagcg gaagaattcc tcgaagcccg attcgtcgag cggctcgatg cgcacggcgg
     2161 cggcctcgac ctgcgcggcc tcggccacgg gctccctgcc cagcca
//
LOCUS       DATCRO010000179         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1104595, whole genome shotgun sequence.
ACCESSION   DATCRO010000179 DATCRO010000000
VERSION     DATCRO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104595"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1007)
                     /locus_tag="VIN75_04805"
     CDS             complement(174..1007)
                     /locus_tag="VIN75_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8973515.1"
                     /translation="MISGKTTLIAHIGFPTESFKAPMIYNPWFDKHGIDAAVIPMGVR
                     PEDYVASLKAILRMSNVRGALVTMPHKVVTNALVDELTPAARIAGASNAILLRPDGTL
                     LGDQFDGAGFVRGVQRKGLALAGKRVLVSGNGGVGSAIAASLAAAGVAQMALFDGSTA
                     ASEALAGRLREHYPKLEIGIGSNDPAGFDLVVNATPLGMKEGDPLPFDMARVAPATFV
                     GEVVMKQEMTPLLLAARAKGCPFQLGTDMLFEMIPAYLEFFGFGSASPEELREVAQLR
                     Y"
     gene            complement(1024..2415)
                     /locus_tag="VIN75_04810"
     CDS             complement(1024..2415)
                     /locus_tag="VIN75_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8973516.1"
                     /translation="MTQEHSTTQTRKATASGWIGSALEYYDFFIYATAASLIFPQIFF
                     PKGNPTTAIVASLATYGVGYVARPIGAFFLGHLGDTHGRKRVLLICMFLMGFSTIAVG
                     LLPTYDQVGLLAPVLLVVLRLIQGFAVAGEISGASSMILEHAPFGRRGFFSSFTLQGV
                     QAGQILAAAVFLPLAHYMPADQFNQWGWRIPFILSFVVIAVAFVIRREVEETPAFSAE
                     ARRGAVPKTPVLEAFRFHWDDMLRVVCCSLMNVIAVVATIFGAAYAVQPAYGVGFSKD
                     VYLWIPVLGNVIAVMIIPMVGNLSDRIGRRPFIIAGALGSGLLSYAYLYAISIHNVPL
                     AICMSLLMWGVVYQGYNAVFPSFYPELFPTRARVSAMAISQNVGTMITAMLPALFASV
                     APPGSANIPLVIGTLTLVTTAISAIAAWTARETYRVALKDLGNRSAAPADKAEYDQQR
                     EVVISQSRWRKAA"
     gene            2586..3356
                     /locus_tag="VIN75_04815"
     CDS             2586..3356
                     /locus_tag="VIN75_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8973517.1"
                     /translation="MSAHKEIDRSLLIEGLGKGLRIIELFSDDHPRLTATEAGMLAGL
                     TRTAARRYLVSLVHYGYAATDGKHYWLLPSILRLGQSYLEAARLPRLVRPFLQRLSMQ
                     TGETANLSVLDGHEIVYLERSNSPRVVSIGFHAGARTPAHVVSPGFAILSTLDKRQLE
                     AWIAEHDFSRFTADTITDAAAIREKVANAARLGYSFVNQYIDIGLGGIAAGLFDRHGH
                     CVGAISMTFQLQRYPEETAIAQLLPPLQDIVKVLREVI"
     gene            complement(3331..>3974)
                     /locus_tag="VIN75_04820"
     CDS             complement(3331..>3974)
                     /locus_tag="VIN75_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8973518.1"
                     /translation="ICMTDASHATVLPRLLAHLRERAPRAPVAAIRIDDDTGAALQSG
                     AADIAVGYIPWLDTGFYQQALFTQDWVCLAAPSNTRIGATLTLRGYREAAHVVTHAGT
                     GAADLEAALARARIERRVMLSLPGFLGLAAIVSTSDLVATLPRQIGEILARNAGLRVL
                     ACPFPVPQFTVKQHWHARFHLEPANRWLREQVAALLMTGPARAARVRSPRAAP"
BASE COUNT          637 a         1328 c         1445 g          564 t
ORIGIN      
        1 ccgccgcgac gagcgtagcc gcgacggggt tcaggcggaa caggacggtg cgggcggtca
       61 tgggatgtct cctcggcgtg gttgtggtgg acaggaaatc gcaaggcgat gcggcagctc
      121 gcccgggccg tggggcggcc cgcgcaggtg tcgcgaaaag ggatgcagcg gcgctagtag
      181 cgcagttgcg cgacctcgcg cagttcctcg ggcgaggcac tgccgaagcc gaagaactcg
      241 aggtaggcgg ggatcatctc gaagagcatg tcggtgccca gctggaacgg gcagcccttg
      301 gcgcgcgccg ccagcagcag cggcgtcatc tcctgcttca tcacgacctc gccgacgaac
      361 gtcgcgggcg cgacgcgggc catgtcgaac ggcagcgggt cgccctcctt catgcccagc
      421 ggcgtcgcgt tgacgaccag gtcgaagccg gccggatcgt tggagccgat gccgatctcg
      481 agcttcggat agtgctcgcg caggcgcccg gcgagcgcct cggacgcggc ggtgctgccg
      541 tcgaacaacg ccatctgcgc gacgcctgca gcggcgagcg aggcggcgat cgcggagccg
      601 acgccgccgt tgcccgacac cagcacgcgc ttgccggcca gcgccaggcc cttgcgctgc
      661 acgccgcgca cgaagccagc gccgtcgaac tggtcgccca gcagcgtgcc gtcggggcgc
      721 agcaggatcg cgttggacgc gccggcgatg cgcgcggcgg gcgtcagctc gtcgaccagc
      781 gcattggtga cgaccttgtg cggcatcgtg accagcgcgc cgcgcacgtt ggacatgcgc
      841 aggatcgcct tgaggctcgc cacgtagtcc tcggggcgca cgcccatcgg gatgacggcc
      901 gcgtcgatgc cgtgcttgtc gaaccacggg ttgtagatca tcggcgcctt gaacgactcc
      961 gtcgggaagc cgatgtgggc gatgagcgtc gtcttgccac tgatcatgat gggttgctcc
     1021 agctcaggcg gccttgcgcc agcgcgactg cgagatcacg acctcgcgct gctggtcgta
     1081 ttcggccttg tccgccggcg cggcgctgcg gttgcccagg tccttcagcg cgacgcggta
     1141 ggtctcgcgg gccgtccagg cggcgatcgc ggagatggcc gtcgtcacca gcgtcagcgt
     1201 gccgatcacc agcgggatgt tggccgaccc cgggggcgcg accgaggcga acagcgccgg
     1261 cagcatcgcg gtgatcatcg tgcccacgtt ctgcgagatg gccatcgccg agacgcgggc
     1321 gcgggtcggg aacagctcgg ggtagaagct cgggaacacc gcgttgtagc cctggtagac
     1381 cacgccccac atcagcagcg acatgcagat cgccagcggc acgttgtgga tgctgatcgc
     1441 atacagatag gcgtacgaca gcaggcccga accgagcgcg ccagcgatga tgaacgggcg
     1501 gcggccgatg cggtccgaca ggttgcccac catcgggatg atcatcaccg cgatgacgtt
     1561 gcccagcacc gggatccaca ggtagacgtc cttgctgaag ccgacgccgt aggccggctg
     1621 caccgcgtac gcggcgccga agatggtggc gacgacggcg atcacgttca tcagcgagca
     1681 gcacaccacg cgcagcatgt cgtcccagtg gaagcggaag gcctccagca cgggcgtctt
     1741 cgggacggcg ccgcgccggg cctcggccga gaacgccggc gtctcctcga cttcccgccg
     1801 gatgacgaag gccacggcga tcacgacgaa gctgaggatg aacgggatgc gccagcccca
     1861 ctggttgaac tggtcggcgg gcatgtagtg ggccagcggc aggaacacgg cggccgccag
     1921 gatctggccg gcctgcacgc cctgcagcgt gaagctggaa aagaagccgc ggcggccgaa
     1981 cggggcgtgc tccaggatca tcgagctcgc gccggagatc tcgccggcca cggcgaagcc
     2041 ctggatcagg cgcagcacga ccagcagcac cggcgccagc aggccgacct ggtcgtaggt
     2101 gggcagcagg ccgaccgcga tcgtcgagaa gcccatcagg aacatgcaga tcagcagcac
     2161 gcgcttgcgg ccgtgggtgt cgcccaggtg gcccaggaag aacgcgccga tcgggcgggc
     2221 cacgtagccc acgccgtacg tcgccagcga cgcgacgatc gcggtcgtcg ggttgccctt
     2281 gggaaagaag atctgcggga agatcagcga cgcggcggtc gcgtagatga agaagtcgta
     2341 gtactcgagc gccgagccga tccagccgct ggccgtggcc ttgcgggtct gggtcgtgct
     2401 gtgttcctgg gtcatgcttg tctccattgg gtggacatcg ggcggatgac gtcggctccg
     2461 cctctggaac gcaatgtaga gagggtgccg gctttttccc aggggtggtg gacgctcgac
     2521 gatcgatcat cgatcgcgta ggatcgctga ttgatcgttc caagggtttc caccgaatca
     2581 acggcatgag cgcgcacaag gaaatcgacc gctcgctgct catcgaaggc ctgggcaagg
     2641 gcctgcgcat catcgagctg ttctccgacg accacccgcg cctgacggcc accgaggccg
     2701 gcatgctggc cggcctcacg cgcacggccg cgcggcgcta cctggtcagc ctggtgcact
     2761 acggttatgc cgccaccgac ggcaagcact actggctgct gccgtccatc ctgcggctcg
     2821 gccagagcta cctggaggcc gcgcggctgc cgcggctggt gcgcccgttc ctgcagcggc
     2881 tgtcgatgca gaccggcgag accgccaacc tgagcgtgct ggacggccac gagatcgtct
     2941 acctggaacg cagcaactcg ccgcgcgtgg tgtcgatcgg cttccacgcc ggtgcgcgca
     3001 cgccggcgca cgtcgtctcg cccggcttcg cgatcctgtc gacgctcgac aagcggcagc
     3061 tggaggcctg gatcgccgag cacgacttca gccgcttcac ggccgacacg atcacggacg
     3121 ccgccgcgat ccgcgagaag gtcgccaacg cggcgcggct gggctactcg ttcgtcaacc
     3181 agtacatcga catcggcctg ggcggcatcg ccgcggggct gttcgaccgc cacggccact
     3241 gcgtcggcgc gatcagcatg accttccagc tgcagcgcta tcccgaggag acggcgatcg
     3301 cgcagttgct gccgccgctg caggacatcg tcaaggtgct gcgcgaggtg atctgacgcg
     3361 cgcggccctg gccgggccgg tcatcaggag cgcggcgacc tgctcgcgca gccagcggtt
     3421 ggccggctcg aggtggaagc gcgcgtgcca gtgctgcttg acggtgaact gcggcaccgg
     3481 gaacgggcac gccagcacgc gcaggccggc gttgcgcgcg aggatctcgc cgatctggcg
     3541 cggcagcgtg gccaccaggt cggacgtcga cacgatggcc gccaggccca ggaagcccgg
     3601 cagcgacagc atcacccggc gctcgatgcg ggcgcgggcc agcgcggcct cgagatccgc
     3661 cgcgccggtg cccgcgtgcg tgacgacgtg cgcggcctcg cggtagccgc gcagcgtgag
     3721 cgtcgcgccg atgcgggtgt tggacggcgc ggccaggcag acccagtcct gcgtgaacag
     3781 ggcctgctgg tagaagccgg tgtccagcca ggggatgtag cccaccgcga tgtcggccgc
     3841 gccggactgc agcgccgcgc cggtgtcgtc gtcgatgcgg atggccgcca cgggcgcgcg
     3901 cggcgcgcgt tcgcgcaggt gcgcgagcag gcgcgggagc accgtggcgt ggctcgcgtc
     3961 ggtcatgcag atgc
//
LOCUS       DATCRO010000180         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_643245, whole genome shotgun sequence.
ACCESSION   DATCRO010000180 DATCRO010000000
VERSION     DATCRO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643245"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..830)
                     /gene="hisF"
                     /locus_tag="VIN75_04825"
     CDS             complement(51..830)
                     /gene="hisF"
                     /locus_tag="VIN75_04825"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY8973519.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDARGLILPIIEAVASQVFIPLTVGGGVREVSDVRRLLNAGADKVSF
                     NSAAVANPQVISDASDKYGAQCIVVAIDAKRRSAEDAAARGPGWDVYTHGGRKNTGLD
                     AVTWAREMVARGAGEILLTSMDRDGTRAGFDLELTRAVSDAVPVPVIASGGVGNLDHL
                     ADGVQQGGADAVLAASIFHYGEHTVGEAKALMAARGIPVRQ"
     gene            complement(1065..1808)
                     /gene="hisA"
                     /locus_tag="VIN75_04830"
     CDS             complement(1065..1808)
                     /gene="hisA"
                     /locus_tag="VIN75_04830"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY8973520.1"
                     /translation="MLLIPAIDLKDGHCVRLEQGDMSVSTSFGDDPAAMARRWVEAGA
                     RRLHLVDLNGAFAGKPVNESAVKAIIKEVNGRIPIQLGGGIRDLDTIERYLDDGLSYI
                     IIGTAAVKSPGFLKDACTAFGGHILVGLDAKDGKVATDGWSKLTGHEVVDLGLKFQDY
                     GVEGVIYTDIGRDGMLTGVNIESTVKLAQALTIPVFASGGLSSMDDIEKLCAVEDEGV
                     EGVICGRSIYTGALDFAKAQSRADELLAG"
     gene            complement(1821..2468)
                     /gene="hisH"
                     /locus_tag="VIN75_04835"
     CDS             complement(1821..2468)
                     /gene="hisH"
                     /locus_tag="VIN75_04835"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY8973521.1"
                     /translation="MTSVVAVVDYGMGNLKSVSQAVVKAAEDSGVDVVIAQSAEQVFA
                     ADRIVLPGQGAIRDCMRELRESGMLEAVLDAAARKPMLGVCVGMQMLLDRSEEQDTAG
                     LGLIPGVVRRFQLEGRLQDDGSRYKVPQMGWNRVRQEFAHPLWAGIPDESWFYFVHSF
                     HVAPSETRHSAGTTEYGDRFTSAVARDNIFATQFHPEKSADLGLALYRNFLHWMP"
     gene            complement(2465..3061)
                     /gene="hisB"
                     /locus_tag="VIN75_04840"
     CDS             complement(2465..3061)
                     /gene="hisB"
                     /locus_tag="VIN75_04840"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY8973522.1"
                     /translation="MTAARIATVQRNTKETQIRVSVNLDGTGVSKLETGIGFFDHMLD
                     QIARHGLIDLEIHAVGDLHIDGHHTVEDVGITLGQAVAKAVGDKKGLTRYGHAYVPLD
                     EALSRVVIDFSGRPGLFMDVDFKSGMIGAFDTQLTYEFFQGFSNHAGVSLHIDNLKGH
                     NAHHQAETIFKAFGRALRMGLTLDPRSLGVIPSTKGSL"
     gene            complement(3168..>3854)
                     /locus_tag="VIN75_04845"
     CDS             complement(3168..>3854)
                     /locus_tag="VIN75_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8973523.1"
                     /translation="PDFELDGPAMLAAVREHQPALLYLAYPNNPTANLWDDGVIDALI
                     EATPGLVVIDEAYQPFAARDSLARLRRHEHVLLMRTMSKFGLAGVRIGYLMGRKPLVA
                     ELDKLRPPFNVSVLNCEAALFALEHVDEYARQAATIRAERDKLLRELQALPGVKPFPS
                     EANMILARVPDAKRVFEGMRARGVLIKNVSGLHPLLADCVRITIGTPEENPQTLAALY
                     GALHDLEATA"
BASE COUNT          575 a         1302 c         1335 g          642 t
ORIGIN      
        1 aggccggacc cctccggccg tgctgcatcc gcacctgact ccaccgcgcc tcactgccgg
       61 acggggatgc cgcgcgccgc catcaacgcc ttcgcctcgc ccaccgtgtg ctcgccgtag
      121 tggaagatgc tggccgccag caccgcatcg gcgccgccct gctgcacgcc gtcggccagg
      181 tggtcgaggt tgccgacgcc gcccgaggcg atcaccggca cgggcacggc gtcggacacc
      241 gcgcgcgtca gctccaggtc gaagccggcg cgcgtgccgt cgcggtccat gctggtcagc
      301 aggatctcgc cggcgccgcg ggccaccatt tccctggccc aggtcacggc gtccagcccc
      361 gtgttcttgc ggccgccgtg cgtgtagaca tcccagcccg ggccgcgcgc ggctgcgtcc
      421 tcggcgctgc ggcgcttcgc atcgatggcc acgacgatgc actgggcgcc gtacttgtcc
      481 gacgcatcgc tgatgacctg cgggttcgcg accgcggccg agttgaagct gaccttgtcc
      541 gcgccggcat tcagcagccg ccgcacgtcc gacacctcgc gcacgccgcc gcccaccgtc
      601 agcgggatga agacctgcga cgccaccgcc tcgatgatcg gcaggatcaa cccgcgcgcg
      661 tcgctggtgg cggtgatgtc caggaacgtg agctcgtcgg cgccctgctc gttgtagcgc
      721 gcggcgatct ccaccggatc gccggcgtcg cgcagttcga cgaagttgac gcccttgacg
      781 acgcggccgc cggtcacgtc caggcagggg atgatgcgtt tggcaagcat ggttctctgg
      841 ggtcaggtct tgaaagttgc gcggcaaggt cctggcgttg gggtcaggtc ttgaaagttg
      901 cacggcagga tcttcgccgc aggacgacag caggtcgtgc aactttcaag acctgacccc
      961 tcggcgaaac gctcaacgcg caactttcaa gacctgacac cctcgagagg cggcggcctg
     1021 cgcaactttc aagacctgac cccggctgat cggaagggat ggtttcagcc cgcgagcagt
     1081 tcatcggcgc gcgattgcgc cttggcgaag tcgagcgcac cggtgtagat cgagcggccg
     1141 cagatgacac cctcgacgcc ctcgtcctcg accgcgcaca gcttctcgat atcgtccatg
     1201 ctggacaggc cgcccgaggc gaacaccggg atggtgagcg cctgcgccag cttgacggtc
     1261 gattcgatgt tgacgccggt gagcatgccg tcgcggccga tgtcggtgta gatcacgccc
     1321 tcgacgccgt agtcctggaa cttcaggccg aggtcgacca cctcgtggcc cgtgagcttg
     1381 ctccagccgt cggtggcgac cttgccgtcc ttggcgtcca ggcccacgag gatgtggccg
     1441 ccgaacgcgg tgcaggcgtc cttcaggaag cccgggctct tgaccgcggc ggtgccgatg
     1501 atgatgtagc tcaggccgtc gtcgaggtag cgctcgatgg tgtccaggtc gcggatgccg
     1561 ccgcccagct ggatgggaat gcggccgttc acttccttga tgatggcctt gaccgccgat
     1621 tcgttgaccg gcttgccggc gaacgcgccg ttcagatcga ccaggtggag ccgccgggcg
     1681 ccggcttcga cccatcgtcg ggccatcgcg gcggggtcgt ctccgaaact cgtggagacc
     1741 gacatgtcac cttgttcgag gcgaacgcag tgaccgtcct tcagatcgat cgcgggaatc
     1801 aacagcatgg ctcgggcccg tcagggcatc cagtgcagga agttgcggta gagcgccagg
     1861 ccgaggtcgg cgctcttctc ggggtggaat tgagtcgcga aaatattatc ccgggccacc
     1921 gccgaggtaa agcggtcgcc atattcggtg gtgcccgcgc tgtggcgggt ctccgacggc
     1981 gcgacgtgga agctgtgcac gaaatagaac cagctctcgt cggggatgcc cgcccacagc
     2041 ggatgcgcga actcctggcg cacgcggttc cagcccatct gcggcacctt gtagcggctg
     2101 ccgtcgtcct gcaggcggcc ctcgagttgg aatcgcctga ccacgccggg tatcaggccc
     2161 aggccggcgg tgtcctgctc ctcgctgcga tcgagcagca tctgcatgcc cacgcacacg
     2221 cccagcatcg gcttgcgtgc cgcggcgtcg agcaccgcct ccagcatgcc ggactcgcgc
     2281 agctcgcgca tgcagtcgcg gatcgcgccc tggcctggaa gcacgatgcg gtcggcggcg
     2341 aagacctgct cggcggactg cgcgatgacg acgtcgacgc cgctgtcctc ggccgccttg
     2401 acgacggcct gcgacacgga cttcaggttc cccatgccgt agtcgaccac ggccacgacg
     2461 gacgtcacag cgaccccttc gtcgacggaa tgacgcccag cgagcgcgga tccagcgtca
     2521 gccccatgcg cagcgcgcgg ccgaaggcct tgaagatcgt ctcggcctgg tggtgggcgt
     2581 tgtggccctt gaggttgtcg atgtgcagcg acacgccggc gtggttcgag aagccctgga
     2641 agaactcgta ggtgagctgg gtgtcgaacg cgccgatcat gcccgacttg aagtcgacgt
     2701 ccatgaacag gcccgggcgg cccgagaagt cgatcacgac gcgcgacagc gcctcgtcca
     2761 gcggcacata cgcgtggccg tatcgggtca ggcccttctt gtcgccgacg gccttggcca
     2821 ccgcctggcc gagggtgatg cccacgtcct cgacggtgtg gtggccgtcg atgtgcaggt
     2881 cgccgacggc gtggatctcc aggtcgatca ggccgtggcg cgcgatctgg tcgagcatgt
     2941 ggtcgaagaa gccgatgccc gtctcgagct tggacacgcc ggtgccgtcg aggttgacgc
     3001 taacgcggat ctgcgtttcc ttggtgttgc gctggacggt ggcgatgcga gcggcggtca
     3061 ttggagcccc tcccgcgccg agtacggcgc tccgtccccc gagggagctg cgggccggct
     3121 cgggacggcc cggcgctcgc cccgcaaata ggttggcacg gaattgttca tgcggtggct
     3181 tccaggtcgt gcagcgcgcc gtagagcgcg gcgagggtct gcgggttctc ttcgggcgtg
     3241 ccgatggtga tgcgcacgca gtcggccagc agcgggtgca ggccggagac gttcttgatc
     3301 aggacgccgc gcgcgcgcat gccctcgaac acgcgcttcg cgtcgggcac gcgcgcgagg
     3361 atcatgttcg cctcgctggg gaacggcttg acgcccggca aggcctgcaa ctcgcgcagc
     3421 agcttgtcgc gctcggcgcg gatcgtcgcg gcctggcgcg cgtattcgtc gacgtgctcc
     3481 agcgcgaaca gcgcggcctc gcaattgagc acgctgacgt tgaacggcgg gcgcagcttg
     3541 tcgagctcgg cgaccagcgg cttgcggccc atcaaatagc cgatgcgcac gccggccagg
     3601 ccgaacttgc tcatcgtgcg catcagcagc acgtgctcgt ggcggcgcag gcgtgcgagc
     3661 gagtcgcgtg cggcgaacgg ctggtaggcc tcgtcgatca ccaccaggcc cggcgtggcc
     3721 tcgatcagcg cgtcgatgac gccgtcgtcc cacaggttgg ccgtggggtt gttcgggtag
     3781 gccaggtaca gcagcgcggg ctggtgctcg cgcaccgcgg ccagcatcgc cgggccgtcg
     3841 agctcgaagt ccgg
//
LOCUS       DATCRO010000181         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_136862, whole genome shotgun sequence.
ACCESSION   DATCRO010000181 DATCRO010000000
VERSION     DATCRO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136862"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VIN75_04850"
     CDS             complement(<1..130)
                     /locus_tag="VIN75_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY8973524.1"
                     /translation="MSTLTLERLNAASRAEFVALLDGTYEHSPWIADAAFDARPFAS"
     gene            complement(133..1080)
                     /gene="puuE"
                     /locus_tag="VIN75_04855"
     CDS             complement(133..1080)
                     /gene="puuE"
                     /locus_tag="VIN75_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEY8973525.1"
                     /translation="MKQSTYPRDLRGYGRNPPHPQWPGNARVAVQFVLNYEEGGENCV
                     LHGDAGSEQFLSEMASPPAFPARHLSMESIYEYGSRVGVWRLLREFERRGLPLTVFGV
                     GMALERCPEVAAAFVEAGHEIACHGWRWISYQAVDEATERAHLQAALESFQRTLGQRP
                     AGWYTGRDSPNTRRLVADAGGFEYDSDYYGDDLPFWLQVRRTDGALAPHLVVPYTLDA
                     NDMRFALPQGYSHGDEFFQYLRDAFDVHYAQGDERPTMMSIGMHCRLLGRPGRLRALQ
                     RFLDHVQRHERVWITRRVDIARHWRQVHPFDAGQAFTWE"
     gene            complement(1077..1808)
                     /locus_tag="VIN75_04860"
     CDS             complement(1077..1808)
                     /locus_tag="VIN75_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8973526.1"
                     /translation="MPRKPAAHLQLVDAPRAGRAAASVTDGIVESITTAIVERRLMPG
                     TRLVEQQIADVFEVSRTVVRQALNQLSRDRLVTLEPARGAFVATPSIDEARQVFQVRR
                     LIEGGMVRQLAAQITDKQVEQLRAHLRDERQAVSRVDVSGRTRLLADFHVVLARLLGN
                     EVLAELIADLLSRSQLIALMYQSSHSAEHSQEEHVDIVDALARRDGRAAARLMERHLE
                     AVERNLRLDPRTPDLASVLRPRAEP"
BASE COUNT          306 a          680 c          785 g          317 t
ORIGIN      
        1 ggctggcgaa cgggcgcgcg tcgaacgcgg cgtcggcgat ccacggcgaa tgctcgtaag
       61 tgccgtccag gagcgcgacg aactccgcgc gcgatgcggc gttgagccgc tcgagggtga
      121 gggtggacat ggtcattccc aggtgaaggc ctggccagcg tcgaagggat gcacctgcct
      181 ccagtgccgc gcgatgtcga cgcggcgggt gatccacacg cgctcgtggc gctgcacgtg
      241 gtcgaggaag cgctgcagcg cgcgcaggcg gccggggcgt ccgagcaggc gacagtgcat
      301 gccgatgctc atcatggtgg ggcgctcgtc gccctgcgcg tagtgcacgt cgaaggcgtc
      361 gcgcaggtac tggaagaact cgtcgccgtg gctgtagccc tgcggcagcg cgaagcgcat
      421 gtcgttggcg tccagcgtgt agggcacgac gaggtgcggg gcgagcgcgc cgtcggtcct
      481 gcgcacctgc agccagaagg gcaggtcgtc gccgtagtag tcgctgtcgt actcgaagcc
      541 gccggcgtcg gcgaccaggc ggcgcgtgtt ggggctgtcg cggccggtgt accagcccgc
      601 ggggcgctgg cccagcgtgc gctggaacga ttcgagcgcc gcctgcagat gcgcgcgctc
      661 ggtggcctcg tccacggcct ggtacgagat ccagcgccag ccatggcacg cgatctcgtg
      721 gcccgcctcg acgaacgcgg cggccacctc ggggcagcgc tccagcgcca tgcccacgcc
      781 gaacacggtc agcggcaggc cgcggcgctc gaactcgcgc aggaggcgcc agacgcccac
      841 gcgcgagccg tactcgtaga tgctctccat gctcaggtgg cgcgccggga aggcgggcgg
      901 gctggccatc tcggacagga actgctcgct gccggcgtcg ccgtgcagca cgcagttctc
      961 gccgccttcc tcgtagttca ggacgaactg cacggcgacg cgggcgttgc ccggccactg
     1021 cgggtggggc gggttgcggc cgtagccgcg caggtcacgc gggtaggtgg attgcttcat
     1081 ggctcagccc tgggacggag gacggacgcg aggtccggcg ttcgcggatc gaggcgcagg
     1141 ttgcgttcga cggcctcgag atggcgttcc atcaacctgg ccgctgcgcg gccgtcgcgc
     1201 ctggccagcg catcgacgat gtccacgtgc tcctcctgcg agtgctcggc cgaatggctg
     1261 gactggtaca tcagcgcgat cagctgcgag cgcgagagca ggtcggcgat gagctcggcg
     1321 agcacctcgt tgcccagcag gcgtgcgagc accacgtgga agtcggcgag caggcgcgtg
     1381 cggcccgaca cgtccacgcg cgacacggcc tggcgctcgt cgcgcaggtg ggcgcgcagc
     1441 tgctccacct gcttgtcggt gatctgcgcg gcgagctggc gcaccatgcc gccctcgatc
     1501 aggcggcgca cctggaacac ctggcgcgcc tcgtcgatgc tgggcgtggc cacgaacgcg
     1561 ccgcgcgccg gctccagcgt gacgaggcgg tcgcggctga gctggttcag cgcctgccgc
     1621 acgacggtgc gcgagacctc gaacacgtcg gcgatctgct gttccaccag gcgcgttccg
     1681 ggcatcagcc ggcgttcgac gatggccgtg gtgatcgatt cgacgatgcc gtcggtgacg
     1741 ctcgccgcgg cgcggcccgc gcgcggagca tcgacgagtt gcaggtgggc ggcgggcttg
     1801 cgcggcatgg gtcgatgctg aatcgtggat tggatgcaaa agtgtataca gttaatcgca
     1861 ccgaatgcaa gcacttcacg ccatcatggt gccgccgtcg cgccgtcggt gtgcacgaaa
     1921 gatggcgttg ccacaggaga agccggtggc gggtcggcac aacgaccaag ggttatccct
     1981 tgctcgagtc gcatcgataa ctgtatacag tcgtcaccat gtcgcagcgg gtgcggcgct
     2041 ccctggcggg tcggttcgac gcgcgaacgg cgggccgttg gacgcgac
//
LOCUS       DATCRO010000182         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_823409, whole genome shotgun sequence.
ACCESSION   DATCRO010000182 DATCRO010000000
VERSION     DATCRO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823409"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..565
                     /gene="coq7"
                     /locus_tag="VIN75_04865"
     CDS             2..565
                     /gene="coq7"
                     /locus_tag="VIN75_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY8973527.1"
                     /translation="MPAPAGATPDALLPAELGPEDKRLSGALMRVNHVGEVCAQALYQ
                     AQAVATSDPALKAQMAQAAREEIDHLAWTEQRLAELGDRPSLLNPLWYGGAFLIGLAA
                     GRLGDRVSLGFVVETERQVEQHLESHLERLPAADAASRAIVDQMRVDEAAHGDAARAA
                     GGVDLPAPVRGAMRMAARVMTATAHYI"
     gene            complement(576..998)
                     /locus_tag="VIN75_04870"
     CDS             complement(576..998)
                     /locus_tag="VIN75_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8973528.1"
                     /translation="MHCTINWLPGTGMAFSAETGSGHLLTMDGAPDGGGRNLAPRPME
                     TLLAGAGGCTAYDVVLILQRGRHDVKGCQVKATAERAEQDPKVFTKLHLHFTVTGRGV
                     PPAAVERAIALSHEKYCSATVMLGKTAEVTTSFDVVEA"
     gene            1441..>2454
                     /gene="ilvA"
                     /locus_tag="VIN75_04875"
     CDS             1441..>2454
                     /gene="ilvA"
                     /locus_tag="VIN75_04875"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY8973529.1"
                     /translation="MSAARPLRESSSDADTGRSSGIAEIAPAPPAALSKSAQAIASAY
                     LRRILTARVYDVAIETPLEPARNLSRRIGNHVLLKREDTQSVFSFKLRGAYNKMIHLA
                     PEALQRGVICASAGNHAQGVALGARRLGVRAVIVMPVTTPRLKIDAVHALGGEVVLHG
                     DSYSDAYGRALQIEKDEGLTFVHPFDDPDVIAGQGTVAMEVLRQHQGPIDAVFVAIGG
                     GGLISGVAAYIKALRPEIKVIGVQAADSDAMIQSVRKGKRVTLSDVGLFSDGTAVKLV
                     GAETFRVARALVDEYITVDTDAACAAIKDVFQDTRSILEPAGALGVAAIKQYVETHKL
                     KGKT"
BASE COUNT          370 a          870 c          854 g          360 t
ORIGIN      
        1 gctgcccgcg cccgccggcg ccacgccgga cgcgctcctg ccggccgagc tgggccccga
       61 ggacaagcgc ctgtcgggtg cgttgatgcg cgtgaaccat gtcggcgagg tctgcgccca
      121 ggcgctctac caggcccagg ccgtggccac ttcggatccg gcgctcaagg cgcagatggc
      181 gcaggccgcg cgcgaggaga tcgaccacct cgcctggacc gagcagcggc tggccgagct
      241 gggcgaccgg ccgagcctgc tcaacccgct ctggtacggc ggcgcgttcc tgatcgggct
      301 ggccgccggg cggctgggcg accgcgtgag cctgggcttc gtcgtcgaga cggagcgcca
      361 ggtggagcag cacctcgaga gccacctcga gcgcctcccc gcggccgacg cggcctcgcg
      421 ggcgatcgtc gaccagatgc gcgtcgacga ggcggcgcac ggcgacgcgg cgcgcgccgc
      481 cggcggcgtc gacctgcccg cgccggtgcg cggggcgatg cggatggcgg cgcgggtgat
      541 gaccgccacc gcgcactaca tctgacgact ggccgtcagg cctcgacgac gtcgaagctg
      601 gtggtgacct cggccgtctt gcccagcatg acggtggccg agcaatactt ctcgtgcgac
      661 agcgcgatcg cgcgttcgac cgccgcgggc ggcacgccgc ggccggtgac ggtgaagtgc
      721 aggtgcagct tggtgaacac cttggggtcc tgctccgcgc gctcggcggt cgccttcacc
      781 tggcagccct tcacgtcatg gcggccgcgc tgcaggatca gcacgacgtc gtaggccgtg
      841 cagccgccgg cgccggccag cagcgtctcc atcgggcgcg gcgccaggtt gcggccgcca
      901 ccgtcgggcg cgccgtccat ggtcagcaga tggccgctgc cggtctccgc ggagaaggcc
      961 atgcccgtcc cgggcagcca gttgatcgtg cagtgcatgc gtctttcccg acgaaatttg
     1021 gaaccgtctc cattgtgcaa cgtcgagttc ggccctgttt gcctcaggac gctgcaaaac
     1081 gcgttccaaa atggtgcacg agcgtgcttt tcgcaccgtc ggcggacatt cgggaagcct
     1141 ctctacgccc ggcagcgaag agatattgca gtgcaacaac cggtgggata gactggctcc
     1201 attgtcgctg acgcaaacgt ttgccccaga gggtgcgacg gccagccgaa gcgaccgcaa
     1261 gttgtctcct ccacctcctc aaaactggtg gattcggccc gaagctgcaa agctccgggc
     1321 cttttctttg gctgcgtcgc tttcgctgcc agcgtggcat ccgttgacgc atcgagaagc
     1381 cagcgcacac gccgggccgt atcattctgc ggctctgccc acctgcgact ggagcccccg
     1441 atgtctgccg cacgccccct gcgtgagtcc tcctccgatg ccgacacggg acgctcgtcc
     1501 ggcatcgcgg agatcgcgcc cgcgccgccg gcagcgctgt ccaagtcggc ccaggccatc
     1561 gccagcgcgt acctgcgccg catcctgacg gcgcgcgtct acgacgtcgc catcgagacg
     1621 ccgctggagc ccgcgcgcaa cctgtcgcgc cgcatcggca accacgtgct gctcaagcgc
     1681 gaggacacgc agtccgtgtt cagcttcaag ctgcgcggcg cgtacaacaa gatgatccac
     1741 ctggcgcccg aggcgctgca gcgcggcgtc atctgcgcat cggccggcaa ccacgcgcag
     1801 ggcgtggcgc tgggcgccag gcgcctgggg gtgcgcgccg tcatcgtgat gccggtgacc
     1861 acgcccaggc tcaagatcga tgcggtgcac gcgctcggcg gcgaggtggt gctgcacggc
     1921 gacagctatt cggacgccta cggccgcgcc ctgcagatcg agaaggacga gggcctgacc
     1981 ttcgtgcacc cgttcgacga ccccgacgtc atcgcgggcc agggcacggt cgcgatggag
     2041 gtgctgcgcc agcaccaggg cccgatcgac gccgtgttcg tggcgatcgg cggcggcggg
     2101 ctcatctccg gcgtcgcggc ctacatcaag gcgctgcggc ccgagatcaa ggtgatcggc
     2161 gtgcaggccg ccgactccga cgcgatgatc cagtcggtca ggaagggcaa gcgagtcacg
     2221 ctcagcgacg tgggcctgtt ctccgacggc acggccgtga agctggtggg cgccgagacc
     2281 ttccgcgtcg cgcgcgcgct ggtcgacgaa tacatcacgg tcgacaccga cgccgcctgc
     2341 gccgcgatca aggacgtgtt ccaggacacg cgcagcatcc tcgagcccgc gggcgcgctg
     2401 ggcgtggccg cgatcaagca gtacgtcgag acgcacaagc tcaagggcaa gacc
//
LOCUS       DATCRO010000183         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_103117, whole genome shotgun sequence.
ACCESSION   DATCRO010000183 DATCRO010000000
VERSION     DATCRO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103117"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..714)
                     /locus_tag="VIN75_04880"
     CDS             complement(43..714)
                     /locus_tag="VIN75_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8973530.1"
                     /translation="MTPPIPVRIWDLPTRVFHVALAMLVTAAIATAEAGGDWMAWHLR
                     CGEAVLALLAFRWAWGLVGGRWSRFASFLPAPARVVRALRGGADAADAIGHSPLGALS
                     VWAFMLLLSAQVATGLVADDDIATTGPLNGHVSSHLARAASGWHAGWGADLVFALIAL
                     HLAAVAWYTLRRRAPLVRAMWNGDKPLPPGTPASRDGAGPRVLALTLFALACTGVWAM
                     LRWAA"
     gene            847..1302
                     /locus_tag="VIN75_04885"
     CDS             847..1302
                     /locus_tag="VIN75_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8973531.1"
                     /translation="MNSWTPAAVAAAALLACGAAAAQFKSPDDAIEYRQGALAVMGHH
                     FGQLGAMANGKIPYDAAAAKANAELVATLARLPWSAFVEGSDKGETGAKPEIWSQRDK
                     FNADAQKLQDAVAKLQDAARTGKPEALKAAFGPTAQACKSCHDDFRKKH"
     gene            1376..2281
                     /locus_tag="VIN75_04890"
     CDS             1376..2281
                     /locus_tag="VIN75_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8973532.1"
                     /translation="MSHASTLVTAAGGPTLSRIVAGAWRMDSWQFSVQERVRWIEGCL
                     ELGITTFDHADIYGGYGVEALFGEALAAAPHLRERMQIVTKCGIRMVSPARPEHRLGH
                     YDTSAAHVERSVDNSLAALRADSLDVLLIHRPDALMDAEALAECFMRLRAAGKVRHFG
                     VSNFTPSQFALLHRRIPLVTNQIEASPLHIAPFHDGTLDQAQDLGVAPMIWSALAGGR
                     LFTGQDDAARRVRAVLERLAGERGIGVTTLVYAWLLRHPARLIPLTGSRRLDAMREAV
                     AALDVELDAQTWYEIWVAGNGAPCP"
     gene            complement(2372..3097)
                     /locus_tag="VIN75_04895"
     CDS             complement(2372..3097)
                     /locus_tag="VIN75_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY8973533.1"
                     /translation="MTEEIFREDATLVECDAVVTAIDERGVQLDRTVFYPHGGGQAGD
                     RGELVLADGRPVAIADTRKGETPGEIVHVLADAGDRVAFTVGAKVHARIDVARRRAHM
                     RFHTATHLLCALVPHPVDGCSITAEYARLDFHMTDPLDKAELEAGLERLVAESHPVSH
                     RWITDAELDANPGLVRSMSVQPPRGTGRVRLLEIAGVDLQPCGGTHVANTSEIGALVV
                     RKIEKKSASTRRVVLAFKDAATP"
     gene            complement(3121..3972)
                     /locus_tag="VIN75_04900"
     CDS             complement(3121..3972)
                     /locus_tag="VIN75_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY8973534.1"
                     /translation="MSAHDAPRTRGFSQVDVFTATPLLGNPLAVVHDGTGLADDRMAA
                     FARWTNLSETTFLLPPTNPEADYRVRIFTPGGELPFAGHPTLGSCHVWLQAGGRPKRD
                     DVVIQECTIGLVPIRRGARLAFAAPPTSVERADAELLHRVYAALGLEPSRVQETAWLS
                     NGFPQLVLRVDSAATVLALKPDHSALKGLGKVGVVGPHPAGSDVDFEVRFFAAAIGIN
                     EDPVTGSLNANVARWLIDSGRAPARYVAAQGTAMGRAGRIHVERDAQGTWIGGDVAGC
                     IAGSVSI"
     gene            complement(3975..5189)
                     /locus_tag="VIN75_04905"
     CDS             complement(3975..5189)
                     /locus_tag="VIN75_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8973535.1"
                     /translation="MTGSNTTPDTADSPWRLARRCERMNPSVIREILKLTELPGVRSL
                     AGGLPSADTFPVEAMRDAALKVLTDTPREALQYAASEGFAPLREWVSAMLAAKGVDAP
                     ANRVLITNGSQQGLDLVGKVLLDAGNTVAVETPTYLGALQAFNPNEPQFASVGSDADG
                     PLPGDIAALSRDTRFMYVLPNFQNPTGRVVPAERRDAIVAAAQRANLPLVEDNPYGEL
                     WYDQEPPKPIASRWKDGVLYLGSFSKVLAPGLRLGFMVCPPALYPKLLQSKQAADLHT
                     GSFSQRVVHEVIKNGFLDQHIPTIRDRYRTQRDAMDAALKASMPAGCEWRKPSGGMFF
                     WLRLPAGCDAMALLPKAVEAGVAYVPGAAFFAHDPDVRTMRLSFVTLSPADIADAVAK
                     LGNVIKAHVAGA"
     gene            complement(5186..6217)
                     /locus_tag="VIN75_04910"
     CDS             complement(5186..6217)
                     /locus_tag="VIN75_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973536.1"
                     /translation="MRLEPQSALPTSAPGSAAAHAVGAEHPHRARAGLWLGLLGMLIF
                     AGTIPMTRLACGSAQAPQLSPFFVAFGRAGVAGLLSVAYLVLTRAPWPHRRHWPMLVL
                     TVLGNVIGWPLLLGLAVRQVDAMHASVITGLMPLATAACGALMLRQRPSAGFWAFALL
                     GAALVVAFAAWSGAGRPQLGDLLLLGAVIASSIGYITGVKVSTGPDGLTPEQTISWVL
                     VLALPATLPVLLTQWPSADIHAASWGAFAYVALFSMWMGFFAWYRGLAWGGTLRVSQV
                     QLLQPFVSILLSVPLLGEQLRPATLGFSIAVMACVYLGRRMPAGAPQSVSSNPVARAA
                     ARSSSKALP"
BASE COUNT          942 a         2364 c         2384 g          815 t
ORIGIN      
        1 gcctgtcacg gcactgacac cccggcgtgc ttcaggcgcg cgtcaggcgg cccagcgcag
       61 catcgcccag acgccggtgc aggccagcgc gaacagcgtc agcgcgagca cgcgcgggcc
      121 cgcgccgtcg cggctggcgg gagtgccggg cggcagcggc ttgtcgccgt tccacatggc
      181 gcgaacgagc ggcgcgcgcc ggcgcagcgt gtaccaggcg accgcggcca ggtgcagcgc
      241 gatcagcgcg aagacgagat ccgcgcccca gcccgcgtgc cagccgctgg ccgcgcgcgc
      301 caggtggctg ctcacgtggc cgttgagcgg cccggtggtg gcgatgtcgt cgtcggccac
      361 cagcccggtg gccacctgcg ccgacagcag cagcatgaac gcccacaccg acagcgcgcc
      421 cagcgggctg tggccgatcg cgtccgcggc gtccgcgccc ccgcgcagcg cgcgcacgac
      481 gcgcgccggc gcgggcagga agctcgcgaa gcgcgaccag cggccgccca ccaaccccca
      541 ggcccagcgg aacgccagca gcgccagcac cgcctcgccg cagcgcaggt gccaggccat
      601 ccagtcgccg cccgcctcgg cggtggcgat cgccgcggtg accagcatgg ccagcgcgac
      661 gtggaagaca cgcgtgggca ggtcccagat gcggacggga atcggagggg tcaaggcgac
      721 ggtggcgcgt gatcgggtcc gcaacgatac gccacggaca catccggtcg cgccggacct
      781 tcgtacactg ccgcagcccc ggacgccccg cgcgtccgcc cgccacctcc ctcaggatcc
      841 gacacgatga actcctggac gcccgccgcc gtcgcggccg ccgccctcct ggcctgcggc
      901 gcggccgccg cccaattcaa gagccccgac gatgccatcg agtaccgcca gggcgcgctc
      961 gccgtgatgg gccaccactt cggtcagctc ggcgcgatgg ccaacggcaa gatcccctac
     1021 gacgcggcgg ccgccaaggc caatgccgag ctcgtcgcga cgctggccag gctgccgtgg
     1081 agcgccttcg tcgagggcag cgacaagggc gagacgggcg ccaagcccga gatctggtcg
     1141 cagcgcgaca agttcaacgc cgacgcgcag aagctgcagg acgcggtcgc caagctgcag
     1201 gacgccgcca ggacaggcaa gcccgaggcg ctgaaggccg cgttcgggcc gaccgcgcag
     1261 gcctgcaagt cctgccacga cgacttccgc aagaagcact gagcgcgcgg cgcggggccc
     1321 gatcggtcag aatccggcac cccttcgtgc cgtcgtcctg gaaccgatcg ccgccatgtc
     1381 ccacgcctcc acgctcgtca ccgccgccgg cggccccacc ctctcgcgca tcgtcgccgg
     1441 cgcctggcgc atggactcgt ggcagttcag cgtgcaggaa cgcgtgcgct ggatcgaggg
     1501 ctgcctcgag ctgggcatca ccaccttcga ccacgccgac atctacggcg gctacggcgt
     1561 cgaggcgctg ttcggcgagg ccctggctgc cgcgccgcat ctgcgcgagc ggatgcagat
     1621 cgtcaccaag tgcggcatcc gcatggtgtc tccggcgcgg cccgagcatc gcctcggcca
     1681 ctacgacacc tcggccgcgc acgtggagcg ctcggtcgac aactcgctgg ccgcgctgcg
     1741 cgccgactcg ctggacgtgc tgctgatcca tcgcccggac gcgctgatgg acgccgaggc
     1801 gctggccgag tgcttcatgc gcctgcgcgc cgccggcaag gtacgccact tcggcgtctc
     1861 caacttcacg ccttcgcagt tcgcgctgct gcatcggcgc atcccgctgg tcaccaacca
     1921 gatcgaggcc tcgccgctgc acatcgcgcc gttccacgac ggcacgctgg accaggcgca
     1981 ggacctgggc gtcgcgccga tgatctggtc agcgctggcc ggcgggcggc tgttcacggg
     2041 ccaggacgac gccgcgcgcc gcgtgcgcgc cgtgctggaa cgcctggccg gcgagcgcgg
     2101 catcggcgtc accacgctgg tctacgcatg gttgctgcgc catcccgcgc gcctgatccc
     2161 gctgaccggc tcgcgccggc tcgacgcgat gcgcgaggcc gtcgcggcgc tcgatgtcga
     2221 gctcgatgcg cagacctggt acgagatctg ggtggcgggc aacggggcgc cttgcccctg
     2281 atccggggca agcaaggcca tcgcgccgcg cactccatga cggctggcgc tggcgcgcgg
     2341 cggcggcgcg cgagggggtc gccccggagg gtcagggcgt cgccgcgtcc ttgaacgcca
     2401 gaacgacgcg ccgcgtcgag gccgacttct tctcgatctt gcgcaccacc agcgcgccga
     2461 tctccgacgt gttggccacg tgcgtgccgc cgcagggctg caggtcgacg ccggcgatct
     2521 cgagcaggcg cacgcggccc gtgccgcgcg gcggctgcac gctcatgctg cgcacgagtc
     2581 cggggttggc gtcgagctcg gcgtcggtga tccagcggtg cgacaccggg tgcgactcgg
     2641 cgaccaggcg ctcgaggccg gcctcgagtt cggccttgtc cagcgggtcg gtcatgtgga
     2701 agtccagccg cgcgtactcc gcggtgatcg agcagccgtc gaccggatgc ggcacgagcg
     2761 cgcacagcag gtgcgtggcg gtgtggaagc gcatgtgggc gcggcgccgc gcgacatcga
     2821 tgcgcgcgtg caccttcgcg ccgacggtga acgccacgcg gtcgccggcg tccgcgagca
     2881 cgtgcacgat ctcgccgggc gtctcgccct tgcgcgtgtc ggcgatcgcg acggggcggc
     2941 catcggccag cacgagctcg ccgcggtcgc cggcctggcc gccgccgtgc ggatagaaca
     3001 cggtgcgatc gagctgcacg ccgcgctcgt cgatggcggt gacgacggcg tcgcactcga
     3061 cgagcgtcgc atcttcgcgg aagatttcct cggtcacgag aggcctcctt gccgggtttg
     3121 tcagatcgac acgctgccgg cgatgcagcc cgccacgtcg ccgccgatcc aggtgccctg
     3181 cgcgtcgcgt tcgacgtgga tgcggccagc gcgccccatc gccgtgcctt gcgcggcgac
     3241 gtagcgcgcc ggcgcgcggc cgctgtcgat cagccagcgc gcgacgttgg cgttgaggct
     3301 gccggtcacc ggatcctcgt tgatgccgat cgccgcggcg aagaagcgca cctcgaagtc
     3361 gacgtcgctg cccgccgggt gcggcccgac gacgccaacc ttgcccagcc ccttgagcgc
     3421 cgagtggtcg ggcttgagcg cgagcacggt ggcggcgctg tccacgcgca gcaccagttg
     3481 cggaaagccg ttggacagcc acgccgtctc ctgcacgcgc gacggctcca ggccgagcgc
     3541 ggcgtagacg cgatgcagca gctcggcgtc ggcccgttcc acgctggtgg gcggtgccgc
     3601 gaaggcgagc cgcgcgccgc gccggatcgg caccaggccg atcgtgcact cctggatcac
     3661 gacgtcgtcg cgcttgggtc ggccaccggc ctgcagccac acgtggcacg agcccagcgt
     3721 gggatgcccc gcgaagggca gctcgccgcc gggcgtgaag atgcgcaccc ggtagtccgc
     3781 ctccggattc gtgggcggca gcaggaacgt cgtctccgac aggttggtcc agcgcgcgaa
     3841 cgcggccatc cggtcgtcgg cgaggcccgt gccgtcatgc acgaccgcca gcgggttgcc
     3901 cagcagcggc gtggccgtga agacgtcgac ctggctgaag ccgcgcgtgc gcggcgcgtc
     3961 gtgggcgctc atggtcacgc gcccgccacg tgcgccttga tgacgttgcc cagcttggcc
     4021 accgcgtcgg cgatgtcggc cggcgacagc gtgacgaagg acaggcgcat cgtgcgcacg
     4081 tcggggtcgt gggcgaagaa cgcggcgccc ggcacgtagg cgacgccggc ctccaccgcc
     4141 ttgggcagca gcgccatcgc gtcgcagccg gcgggcaggc gcagccagaa gaacatgccg
     4201 ccgctgggct tgcgccattc gcagccggcg ggcatcgagg ccttgagcgc cgcgtccatc
     4261 gcgtcgcgct gcgtgcggta gcggtcgcgg atcgtcggga tgtgctggtc gaggaagccg
     4321 ttcttgatga cctcgtgcac cacgcgctgg ctgaacgagc cggtgtgcag gtcggccgcc
     4381 tgcttggact gcagcagctt ggggtacagc gccggcgggc acaccatgaa gcccaggcgc
     4441 aggcccggcg cgagcacctt ggagaacgag ccgaggtaca gcacgccgtc cttccagcgc
     4501 gaggcgatgg gcttgggcgg ctcctggtcg taccagagct cgccgtacgg gttgtcttcc
     4561 accagcggca ggttggcgcg ctgtgccgcg gcgacgatcg cgtcgcggcg ctccgccggc
     4621 accacgcggc cggtcgggtt ctggaagttc ggcagcacgt acatgaagcg cgtgtcccgg
     4681 gacagcgcgg cgatgtcacc gggcagcggg ccgtcggcgt cgctgccgac cgacgcgaac
     4741 tgcggctcgt tcgggttgaa cgcctgcagc gcgcccaggt aggtgggcgt ctcgacggcc
     4801 acggtgttgc cggcatccag cagcaccttg ccgacgaggt ccaggccctg ctgcgagccg
     4861 ttggtgatca gcacgcggtt ggccggcgcg tcgacgccct tggccgccag catggccgac
     4921 acccactcgc gcagcggcgc gaagccttcg ctggccgcgt actgcagtgc ctcgcgcggc
     4981 gtgtcggtca gcaccttcag cgcggcgtcg cgcatggcct cgacggggaa ggtgtcggcc
     5041 gacggcaggc cgccggccag ggagcgcacg ccgggcagct cggtcagctt gaggatctcg
     5101 cggatgaccg aggggttcat gcgttcgcag cgtcgtgcga ggcgccacgg cgagtcggcg
     5161 gtgtcggggg tggtgttgga accggtcatg ggagagcctt cgaggaagaa cgcgcggcgg
     5221 cccgcgcaac gggattgctg gaaacggatt gcggcgcgcc cgccggcatg cgccggccca
     5281 ggtacacgca agccatcacg gcgatggaga agcccagcgt ggccgggcgc agttgttcgc
     5341 ccagcagcgg cacgctgagc aggatcgaga cgaagggttg cagcagctgc acctggctga
     5401 cgcgcagcgt gccgccccag gcgagcccgc gataccaggc gaagaagccc atccacatcg
     5461 agaacagcgc gacgtaggcg aacgcgcccc acgacgcggc atggatgtct gcgctgggcc
     5521 attgcgtcag cagcacgggc agcgtggccg gcagcgccag cacgagcacc caggagatgg
     5581 tctgctcggg cgtcaggccg tccgggccgg tcgacacctt gaccccggtg atgtagccga
     5641 tggacgacgc gatcaccgcg cccagcagca gcaggtcgcc cagctgcggc cggcctgcgc
     5701 cgctccaggc tgcgaacgcg acgaccagcg cggcgcccag cagcgcgaag gcccagaagc
     5761 cggctgacgg gcgctggcgc agcatcagcg cgccgcacgc ggcggtggcc agcggcatga
     5821 gccccgtgat gaccgacgcg tgcatcgcgt cgacctggcg caccgccagt cccagcagca
     5881 gcggccagcc gatgacgttg cccagcacgg tcagcaccag catcggccag tgccgccggt
     5941 gcggccaggg cgcccgggtg aggacgaggt aggccaccga cagcagcccc gcgacccccg
     6001 cgcggccgaa ggccacgaag aacggcgaca gctgcggcgc ctgcgcgctg ccgcaggcca
     6061 ggcgcgtcat ggggatcgtg ccggcgaaga tcagcatgcc cagcaggccc agccacagcc
     6121 cggcgcgggc acgatgggga tgctcggcgc ccacggcgtg cgccgcggcg gagccggggg
     6181 cggacgtcgg cagggcggac tggggctcga ggcgcatcgg ctcacgacgc gcgccggggc
     6241 ttcgggagga agcggtgaaa cggcgtgcgg aacggccagt ctaggccgat aatcaataca
     6301 ccagcagtac agttgccggg tgcagttcgg tgatctgtat cgctgcccgc ggcagtacag
     6361 atcgaccgat cgacgccgcg acctccagga gctccgccat gtccgacgac gccaccgcct
     6421 ccgccaccac gccgccgcgc gcgcgccggc caggcgcgat gccggccgca ccggccgcca
     6481 tcgcggtggg cgccgcgctg ctggc
//
LOCUS       DATCRO010000184        12435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_395840, whole genome shotgun sequence.
ACCESSION   DATCRO010000184 DATCRO010000000
VERSION     DATCRO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395840"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..393
                     /locus_tag="VIN75_04915"
     CDS             61..393
                     /locus_tag="VIN75_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973537.1"
                     /translation="MNRTRSVLLLATAGLAAAALLRNARRSRTARRIADATMPDRSAL
                     EQAPVHHEDLETYQVLLDESLTLTFPASDPICAQAATRCGDAVATPADPSDWRLHRGS
                     TESNLAGR"
     gene            504..839
                     /locus_tag="VIN75_04920"
     CDS             504..839
                     /locus_tag="VIN75_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973538.1"
                     /translation="MDQQHAAAADKGSPDDRSRNQTRTTHDQSMNTDATGGADGRVGP
                     RVEPALPHDLDESARSQASASAQHVDIGKQALADEMSPSEDTDRGPVLDKVYHEKVAT
                     DRGRTPPRQ"
     gene            complement(845..2038)
                     /locus_tag="VIN75_04925"
     CDS             complement(845..2038)
                     /locus_tag="VIN75_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY8973539.1"
                     /translation="MPTTIVFGAFDRHNLGDLLFAHVAAALLPRDETVVFAGLAARDL
                     RPFGGHRVEALHALRDDPRLRGARLVHAGGEILTCRAHDAAVMLLDPAEVDATLAFLA
                     AHPEGAPAWRRAMLGTDDELPYVVGRDAFRWLASVSLAAVGGVALDALPGDARADLLE
                     RLVSADALTVRDAVTQAQLHAAGIVAPLMPDPAELVETLFGDAIRARVTEPAIAGLRQ
                     AFPGGWLAVQLSTEFADDATLAAIATQLEAVPDEAGLGIVLFRAGAAPWHDDPALLAR
                     LARRIPPRHVRVFDSLDIWDLCALLAHAHACLGSSLHGGIVARAFGVPALGLEPPRPA
                     QGRKLAAYASTWHALEAPVVRHYGEIGASLREALASDPAPRRAHAAERASRAREALQR
                     LLAPT"
     gene            2104..3042
                     /locus_tag="VIN75_04930"
     CDS             2104..3042
                     /locus_tag="VIN75_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006757047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8973540.1"
                     /translation="MSKRPGALRIGISGWRYPAWRGVFYPKGLPQRAELEYAASRMNS
                     AEINGSFYSLQHAASWQAWHAQTPEDFIFAVKGPRYVTHMLKLRNARTAVANFIASGV
                     LALHDKLGPMLWQLPPNLAFHADRMEEFLALLPRDTDAARALGREHDARLDGRTWLPE
                     GPRRALRHAFEIRHPSFADPAFVALLRRHGAALVVADTASRWPLLEDVTADFVYVRLH
                     GDEALYTSGYGDAALAGWARRISAWRHGGQVEDARTTSAPPPRAVSRRDVYCYFDNDV
                     KVHAPFDAMRLAAACEEGTSTRPTSAPRARRLPSAD"
     gene            complement(3164..3568)
                     /locus_tag="VIN75_04935"
     CDS             complement(3164..3568)
                     /locus_tag="VIN75_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973541.1"
                     /translation="MNAILKLTTVAIGAIGIAGGVALAQTSSTSNSTGTTSNNNSSAM
                     PSNSSTSTDTNSNSNNTSTSPSSSSTNSTNGYGTNSNSNSNSNLGTDTTTNTGTNSST
                     TNSSSSSYDNNANNASSNSSSNNNLRPRADRN"
     gene            3614..4213
                     /locus_tag="VIN75_04940"
     CDS             3614..4213
                     /locus_tag="VIN75_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEY8973542.1"
                     /translation="MRVSGNGDALDRNDDCFPTRGRATRAPKKAGDALELRPIEEKVP
                     NAAGRASSAGRLQASAPWPSGPGIEMLIRHFYVSQIADGLSDLEVQVILGVAQMMNRR
                     LDLTGMLVQSDGHFAQVLEGRAEAVATLMTRIRRDPRHRNVRTLLEEPILRRRFASWA
                     MRLVRPDDMDGLLRDACRDGCQDDMAARRLMQALVDRLP"
     gene            4410..4874
                     /locus_tag="VIN75_04945"
     CDS             4410..4874
                     /locus_tag="VIN75_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973543.1"
                     /translation="MNSNQQGQDDGRSESLGQGTGQQEQSGSQGQQGSQSSQQGRENL
                     ASSGMGQSGGLDSQQSQSGSSSQQSPSGGSRSQQGQSGGLGQQSQSASSGSQQGGTGS
                     LGSQQSQSGGASGQQGSSSGIGQSSQSEGSQRDSSLQQGSDNTNRSSGEQSR"
     gene            5045..5593
                     /locus_tag="VIN75_04950"
     CDS             5045..5593
                     /locus_tag="VIN75_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8973544.1"
                     /translation="MSVLDKVVAAITPTESEDERRDARAKAEAVAANCDWLRLILAHH
                     RAIEDAFAQLAAASDANGRRRAQKWLATLLNGHSMAEEAVIYPAMALGDQKTHATTAY
                     TEQSGAKVNLAALEGLDPMSQDYADKCEHVRGAVMHHMYSEEDDWLIDLAKTEDAGLH
                     SRLSARYAEEFNRYMGPDAAMP"
     gene            complement(5642..6592)
                     /locus_tag="VIN75_04955"
     CDS             complement(5642..6592)
                     /locus_tag="VIN75_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973545.1"
                     /translation="MDLRLKPVDQQVIVITGASSGIGLATALAAAHQGARVVLGARSV
                     DVLDDVVAHIAAEGGQAVAVETDVSDRGQLEQLANRALDAFGRIDTWVNNAGLSIYGR
                     LDEVDEAHSRRLFDINFWGVVNGSLVALPHLRVQGGALINLGSEASEAAVPLQAMYSA
                     SKHAVKGFTDGLRLELEADGAPVSVTLIQPTAVDTPYPEHAANYLAQEPKLPSPMIDV
                     EKVASAILDAAASPTRSTKVGAMSVVNTAMAKFIPSLADVMARKQIGRQQRDEAPHSR
                     DGTLYEAGHAGRVRGRGNEDTTDAKRAEAMNTHPAEGHAS"
     gene            complement(6652..7557)
                     /locus_tag="VIN75_04960"
     CDS             complement(6652..7557)
                     /locus_tag="VIN75_04960"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8973546.1"
                     /translation="MTPARALAGAAAAFASARCEAHLPANADHASPAFPWSFEPWIVV
                     LLAASMALYVLGTTRLWRRAGAGHGLGAGTVAAWTAGWLALVGALVSPLDPLGESLFS
                     AHMVQHEMLMVVAAPLLVLGRPLAAWTWALPAAWRPRVGAATKRPRWRRFWSHLTHPL
                     TAWGLHALALWGWHLPWLFDAALAHPATHALQHASFLGTALLFWWTTLGGERRALRGA
                     AIASLFTTMLHTGALGALMTLSPLLWYGRYAATSAVYGLDPLADQQLGGLIMWIPASV
                     AYLAVGLALVGRALSSRPASLSPLP"
     gene            complement(7590..7955)
                     /locus_tag="VIN75_04965"
     CDS             complement(7590..7955)
                     /locus_tag="VIN75_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973547.1"
                     /translation="MTRRLRSWPALLLAPLTALAQQSICLSLLKHACEQQTTAPVHAV
                     SAVSLVAILAMTWLAAAEWLASRALTDHRPGAAQRDDTTPTRLAHFLAGVGMLVGGLS
                     ALVALFMWMPVWVLNPCMD"
     gene            complement(7961..8620)
                     /locus_tag="VIN75_04970"
     CDS             complement(7961..8620)
                     /locus_tag="VIN75_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8973548.1"
                     /translation="MSISEPWDPASRRTRGGGAAADGPALDVARLPSFGFGTRSLMWW
                     GTLGLMAVEGTVFALAVATYFYLRGQSKVWPLNEPPPSLLWGSLNTVLLLLSLWPNHL
                     AKRAAERFDRRGARLWLGVCLLAALAFLVLRGLEFTTLHCRWYEDAYASAVWLLLGLH
                     TTHLVTDTWDTAVLLALARVGPFEAKRHVDVSENAVYWVFVVLSWLPIYAVIYWAPRV
                     V"
     gene            complement(8617..10533)
                     /gene="ctaD"
                     /locus_tag="VIN75_04975"
     CDS             complement(8617..10533)
                     /gene="ctaD"
                     /locus_tag="VIN75_04975"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8973549.1"
                     /translation="MRPPGNDVPPTPAQEYALDRAWSDPPGPGGWISAVNHKAIGKRF
                     IVTAFGFFVAAGALALLMRLQLARPESKLMGPDFYNQVFTMHGTTMMFLFAVPVMQAM
                     SVYLVPLMIGTRSVAFPRMNAFAYWIYLFGGLMLWVAFACNTGPDAGWFSYVPLAGPD
                     FSPGKRVDFWAQMITFTEVSGVLEAIILITTVFKLRAPGMTLNRMPLFVWGMLVTAFM
                     VMFAMPAVILSSTNLILDRLVGTHFYNPGEGGDALLWQHLFWFFGHPEVYFIFLPGLG
                     FVSTIIPTFARRPVFGYTAMVLSLIATGFLAFGLWVHHMFATNLPEMGKSFFTAASML
                     IAIPTAVQIFCWIATLWTGRPVFRTPLLFILGFFFILVLGGLTGVMLAAVPLDLQIHD
                     TYFVVAHLHYVLIGGAVFPLFGAVYYWYPKFTGRRMGERLGRWNFWLFFIGFNAAFFP
                     MHLLGLAGMPRRVWTYPKGMGWGDMNFVATLGALTIGVSVVLFLLNCWRSRRQGEPAG
                     DNPWNAGTLEWAAASPPHAANFPAIPVVHGRDPLWESARMPNQVAGLSVEDREVLVTS
                     VLDAAPETRVTFPAPTSWPFWSAVATTVLFVGSIFTPWAVVWASIPVAIAMTFWFWPK
                     GAEVRRSVALEQRP"
     gene            complement(10560..11606)
                     /gene="coxB"
                     /locus_tag="VIN75_04980"
     CDS             complement(10560..11606)
                     /gene="coxB"
                     /locus_tag="VIN75_04980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8973550.1"
                     /translation="MARAERLRDAALLAACALEAALARAGTPTSPLQDVLRPGGPQAQ
                     HVASLWHLMLAICTLVFVAVMVALVIALLKRPHGRLDAPPDLASLSVPEPGAHRSVAT
                     AIGASTVLLLFLIVASVLTDRALANISTKGALTLKVTADQWWWQVTYDGPPSEAFDTA
                     NEIHVPVGRPVVVRLQADDVIHSLWVPSLAGKKDLIPGRDATLQFRADKPGIYRGQCA
                     EFCGAEHAWMAFEVVAEPPERYAAWVDHQRQPAPPPGDPLRQKGQTLFVSTTCAMCHA
                     VRGTTASARHAPDLTHLASRRTLAAGTLPNTPEALRAWIRDPHKFKPGVNMPATNMPD
                     ADLDAIVAWLGTLS"
     gene            complement(11593..12183)
                     /locus_tag="VIN75_04985"
     CDS             complement(11593..12183)
                     /locus_tag="VIN75_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8973551.1"
                     /translation="MSSRFSERRAAWLVLAFGCAAMLAACEREKRDFDPPQGKAQAPR
                     TRNTEFQPGDPAPVAPPAAPAEPDTPYTVEGNAYAVSQGKRLYRWYNCSACHAPGGGG
                     DWGPALSDAKWIYGSRPEDIYASIVQGRPAGMPSFGAHIPQDQVWDLVAYVRSLSGQL
                     RIDVAPGRSDGMAPREPETRRTQEIPTPQVIDDGTR"
     gene            complement(12164..>12435)
                     /locus_tag="VIN75_04990"
     CDS             complement(12164..>12435)
                     /locus_tag="VIN75_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY8973552.1"
                     /translation="FKAVDARDGRPLWQYKVDSGIVGQPISYRGPDGHQYVAVVSGVG
                     GWAGKVVSQDLDVRDRTAGKAFAGAMRDLPQYTKKGGTLYVFSLQ"
BASE COUNT         1925 a         4321 c         4539 g         1650 t
ORIGIN      
        1 cgcggcgcgc gccggcgtgt cggcagcgcc atgtcattcg actgatccct ggagaccgcc
       61 atgaaccgca cccgatccgt tctgcttctc gcgaccgccg gcctcgccgc tgctgcactg
      121 ctgcgcaatg cacggcgttc ccgcacggcg cgtcgcatcg ccgacgccac catgcccgac
      181 cgctccgcgc tcgagcaggc gcccgtccac cacgaggacc tggagaccta ccaggtgctg
      241 ctcgacgagt cgctgaccct gacctttccg gccagcgacc cgatctgcgc gcaggctgcc
      301 acgcgctgcg gtgacgccgt ggcgacgccg gccgatccgt ccgactggcg gctgcaccgc
      361 ggctcgaccg agtcgaacct ggccggacgc tgaaggggcg gcccgttgcc gaaggccttg
      421 ccgctggaca tccgttcctg gaagcaatgg ccgaccggta catccattgc ccggcaaggg
      481 cagacgcaat gaatggagcc gacatggacc agcaacacgc cgccgccgca gacaagggct
      541 cgccggacga ccgatcgcgc aaccagacgc gcacgaccca cgaccagagc atgaacaccg
      601 atgcgaccgg aggcgccgac ggccgcgtcg gcccccgcgt cgaaccggcg ctgccgcatg
      661 acctggacga gtccgcgcgc agccaggcca gcgccagcgc gcagcacgtg gacatcggca
      721 agcaggcgct ggctgacgag atgagcccgt cggaggacac ggatcgaggc ccggtgctcg
      781 acaaggtcta ccacgagaag gtcgcgactg accgcgggcg cacgccgccg cggcagtgaa
      841 cgcgtcaagt cggcgcgagc agccgctgca gcgcctcgcg cgcgcgtgac gcgcgctcgg
      901 ccgcgtgcgc gcggcgcggc gcgggatcgc tcgcgagggc ctcgcgcagg ctggcgccga
      961 tctcgccgta gtgccgcacg accggcgctt cgagcgcgtg ccacgtcgag gcgtacgcgg
     1021 cgagcttgcg gccctgcgcc ggccgcggcg gctccaggcc gagcgccggc acgccgaacg
     1081 cgcgggccac gatcccgccg tgcaggctgc tgcccaggca cgcgtgcgca tgggccagca
     1141 acgcgcacag gtcccaaatg tcgagcgagt cgaacacgcg cacgtggcgc ggcgggatgc
     1201 ggcgcgcgag ccgcgcgagc agcgccgggt cgtcgtgcca gggtgcggcg ccggcgcgaa
     1261 acaggacgat gcccagtccc gcctcgtccg gcacggcctc cagctgcgtg gcgatggcgg
     1321 ccagcgtcgc gtcgtcggcg aactcggtcg acagctgcac cgccagccag ccgccgggaa
     1381 acgcctggcg cagtccggcg atcgccggct cggtgacgcg cgcgcggatc gcgtcgccga
     1441 acaacgtctc caccagttcg gccggatcgg gcatcaatgg cgcgacgatg ccggccgcgt
     1501 gcagctgcgc ctgggtgacg gcgtcgcgca cggtcagtgc atccgcgctc accaggcgtt
     1561 cgagcaggtc cgcccgtgcg tcgccgggga gcgcatcgag cgcgacgccg ccgacggcgg
     1621 ccagcgagac cgacgcgagc cagcggaacg catcgcgtcc gacgacgtag ggcagctcgt
     1681 cgtccgtgcc cagcatcgcc cgccgccacg cgggcgcgcc ctcgggatgg gccgcgagga
     1741 acgccagcgt ggcgtcgacc tccgccgggt cgagcagcat gacggcggcg tcgtgcgcgc
     1801 ggcaggtcag gatctcgccg ccggcatgca cgaggcgagc gccgcgcagc cggggatcgt
     1861 cgcgcagcgc gtgcagcgcc tcgacgcgat ggccgccgaa cggtcgcagg tcgcgcgcgg
     1921 cgaggccggc gaagaccacc gtctcgtcgc gcggcagcag cgccgccgcg acgtgcgcga
     1981 acagcaggtc gccgaggttg tggcggtcga aggcgccgaa gacgatcgtg gtgggcatgg
     2041 cgtgcggcca gcgtagccga aatcgcggcc ggccgcaggg catcggcctt gccgaagccg
     2101 ggcatgagca aacgtcccgg cgcgctgcgc atcggcatct cgggctggcg ctatcccgcc
     2161 tggcgcggcg tcttctaccc gaagggcttg ccccaacgcg ccgagctcga gtacgcggcc
     2221 agccggatga acagcgccga gatcaacggc tcgttctact cgctgcagca cgcggcgtcg
     2281 tggcaggcct ggcacgcgca gacgcccgag gacttcatct tcgccgtgaa gggaccgcgc
     2341 tatgtcacgc acatgctcaa gctgaggaac gcccgcacgg cggtcgcgaa cttcatcgcc
     2401 tcgggcgtgc tggcgctgca cgacaagctg ggcccgatgc tgtggcaact gccgcccaac
     2461 ctggcgttcc atgccgaccg gatggaggag ttcctggcgt tgctgccgcg cgataccgac
     2521 gccgcgcgcg ccctcggccg cgagcacgat gcgcgcctcg acggccgcac gtggttgccc
     2581 gagggcccgc gccgcgcact gcggcacgcg ttcgagatcc ggcacccgag cttcgccgat
     2641 ccggccttcg tcgcgctgct gcggcgtcat ggggcggcgc tggtcgtggc cgacaccgcg
     2701 tcgcgctggc cgctgctgga ggacgtgaca gccgacttcg tctacgtgcg gctgcacggc
     2761 gacgaggcgc tctacacgag cggctacggc gatgccgcgc tggccggatg ggcgcgccgg
     2821 atctccgctt ggcgccacgg cggccaggtc gaggacgcgc gcacgacgtc ggcacccccg
     2881 ccgcgcgcgg tgtcgcggcg cgacgtctac tgctacttcg acaacgacgt gaaggtgcat
     2941 gcgccgttcg acgcgatgcg gctggccgcg gcgtgcgagg agggcacctc gacgaggccg
     3001 acgtccgcgc cacgcgcgcg ccggctgccg tccgccgatt gatggccgag gcggcgcccg
     3061 gtgcgcttcg caggccatgg ggatggcggt cgggcggcgc aggcggggcg ggtcatcgaa
     3121 gcgatgcccg ccgccaggtc acgtcgcgtg gacgcggtcg ccgtcagttg cggtcggcgc
     3181 gcggcctcag gttgttgttg gacgacgagt tcgagctcgc gttgttcgcg ttgttgtcgt
     3241 agctgctcga cgagctgttc gtcgtgctgg agttcgtccc cgtgttggtg gtggtgtccg
     3301 tgcccaggtt cgagttcgag ttggaattcg aattcgtgcc atagccgttc gtcgagttcg
     3361 tggagctgct gctcggcgag gtgctggtgt tgttcgagtt cgaattggtg tccgtgctcg
     3421 tcgacgagtt gctcggcatc gcgctcgaat tgttgttcga cgtggtgccg gtgctgttgc
     3481 tcgtgctgct ggtctgcgcc agggcgacgc cgcccgcgat gccgatggcg ccgatggcga
     3541 cggtggtcag tttgaggatc gcattcatgt gaagtctccg tgggggtggt tggcatccac
     3601 gcgaatgtcg tggatgcggg tttccggcaa cggcgatgcc ctcgaccgga atgacgattg
     3661 cttcccgaca agggggcgcg caacccgcgc gcccaaaaag gcgggtgatg ccctggagtt
     3721 gcgtcccatc gaggaaaagg taccgaacgc ggctggtcgt gcatccagtg ccgggcggct
     3781 gcaggcctcg gcgccttggc cgagcgggcc cggaatcgag atgctgatcc gccatttcta
     3841 tgtcagccag atcgccgatg gcctgtccga cctggaggtc caggtcatcc tcggcgtcgc
     3901 gcagatgatg aaccggcggc tcgacctcac cggcatgctc gtgcagagcg acgggcactt
     3961 cgcacaggtg ctcgaagggc gtgcggaggc cgtggccacg ctgatgacgc ggatcaggcg
     4021 cgacccgcgc catcgcaacg tgcgcacgct tctggaggag ccgatcctgc gtcgccggtt
     4081 cgcgagttgg gcgatgcgcc tcgtgcggcc ggacgacatg gacggactct tgcgcgacgc
     4141 atgccgcgac ggctgccagg acgacatggc ggcgcgtcgg ctcatgcagg cgctggtcga
     4201 ccggctgccg tgaatcgccc gcccgtcggg ccgtcggcgc gccgcgcatc acgtcggcgt
     4261 caacgagcct gcgcacggac tgcgcgttcg ttcagggcga tcagggacgg attgggtaca
     4321 gccgttgcca gatcgggtgg cgctggaacg atggcgacgc aggtcgcttc gggcggcgaa
     4381 accattcatc aatcgatcaa ggagccaaca tgaactcgaa ccagcaaggc caggacgacg
     4441 gccgctcgga atccctcggc cagggcaccg gccagcagga acaatcgggc tcgcagggcc
     4501 agcaaggcag ccagtccagc cagcagggcc gcgagaatct cgcctccagc ggcatgggcc
     4561 agtcgggcgg cctggacagc cagcagagcc agtcgggcag cagcagccag cagagcccgt
     4621 cgggcggctc ccgcagccag cagggccagt ctggcggcct gggccagcag agccagtccg
     4681 caagctccgg cagccagcag ggcggcaccg gaagcctcgg cagccagcaa agccagtcgg
     4741 gcggtgcttc gggccagcag ggctcgagca gcggcatcgg ccagtcgtcg cagtcggaag
     4801 gctcgcagcg tgactcgtcg ttgcagcagg gcagcgacaa cacgaatcgc agcagcggcg
     4861 agcagagccg ctgaggcgag cgcgggcggc tttcgggccg cccgcctgga agccgcggcg
     4921 cgccgcggct tcaatccgct tgaagcagga attccggccg ccgccgccgt catttgcgga
     4981 aagcagatcg gcggccgcgg gcgccacacc cgatgcaggc cgcagtgccc aaggagtcca
     5041 tcccatgtcc gttctcgaca aggtcgtcgc ggcgatcacg cccaccgaat ccgaagacga
     5101 gcgccgcgat gcgcgcgcca aggccgaagc cgtcgccgcc aactgcgact ggctgcgcct
     5161 gatcctcgcg caccatcgcg cgatcgagga cgcgttcgcg cagctcgccg cggcctccga
     5221 cgccaacggc cgccggcgtg cgcagaagtg gctcgccact ttgctcaacg gccattcgat
     5281 ggccgaggag gcggtgatct acccggcgat ggcgctcggc gaccagaaga cgcacgcgac
     5341 caccgcctac accgagcaga gcggcgccaa ggtcaacctg gccgcgctcg aagggctcga
     5401 tccgatgagc caggactacg ccgacaagtg cgagcacgtg cgcggcgccg tgatgcacca
     5461 catgtactcg gaggaggacg actggctcat cgacctcgcc aagaccgagg acgcgggcct
     5521 gcattcgcgc ctgagcgcgc gctatgccga ggagttcaac cgctacatgg gtcctgacgc
     5581 ggcgatgccc tgaagcgccc gaaggcgcgg cggccgaacg gccggccacg ccggcgccag
     5641 gtcaggacgc gtgcccttcg gccgggtgcg tgttcatcgc ctcggcgcgc ttcgcgtcgg
     5701 tcgtgtcctc gttcccgcgg ccgcgcacgc ggcccgcatg gcccgcctcg tagagcgttc
     5761 cgtcgcgcga gtgcggcgcc tcgtcgcgct gctggcggcc gatctgcttg cgcgccatga
     5821 cgtcggccag gctgggaatg aacttggcca tcgcggtgtt gacgacggac atcgcgccga
     5881 ccttggtgga gcgcgtcggg gacgccgcgg cgtcgaggat cgcgctggcc accttctcca
     5941 cgtcgatcat cggcgatggc agcttgggct cctgcgccag gtagttggcg gcgtgttcgg
     6001 ggtagggcgt gtcgaccgcc gtgggctgga tcagcgtgac cgataccggc gcgccgtcgg
     6061 cctccagctc cagccgcagg ccgtcggtga agcccttgac cgcgtgcttg ctggccgagt
     6121 acatcgcctg cagcggcacg gccgcctccg aggcctcgct gccgaggttg atcagcgcgc
     6181 cgccttgcac gcgcaggtgc ggcagcgcga cgagcgagcc gttcacgacg ccccagaagt
     6241 tgatgtcgaa cagccggcgg ctgtgcgcct cgtcgacctc gtcgagccgg ccgtagatcg
     6301 acagcccggc gttgttgacc caggtgtcga tgcggccgaa tgcgtcgagc gcgcggttgg
     6361 ccagctgctc gagctgtccg cggtcggata cgtccgtctc gacggcgacc gcctggccgc
     6421 cctcggcggc aatgtgcgcg acgacgtcgt ccagcacgtc gacgctgcgc gcgcccagta
     6481 cgacacgcgc gccctggtgc gccgcggcca gcgcggtggc gagaccgatg ccgctggagg
     6541 cgccggtgat gacgatgacc tgctggtcga cgggcttgag gcggagatcc atggcgagtc
     6601 ctcgggaaag gggtggcgag gccctgccgt ggcaaggcgg tttcccgcgc atcagggcaa
     6661 cggcgacagc gacgccggtc ggctggacag cgcgcggccg accagggcga ggccgaccgc
     6721 gagataggcc accgaggccg ggatccacat gatcaggccg cccagttgct ggtcggccag
     6781 cgggtcgagg ccatacaccg ccgacgtcgc ggcatagcgc ccgtaccaga gcagcggaga
     6841 cagcgtcatc agcgcaccga gcgcgccggt atgcagcatc gtcgtgaaca gcgatgcgat
     6901 ggccgccccg cgcagcgccc ggcgttcgcc gccgagcgtc gtccaccaga acagcagcgc
     6961 cgtgccgagg aagctcgcgt gctgcagcgc gtgcgtggcc gggtgggcca gcgccgcgtc
     7021 gaacagccat ggcaggtgcc agccccacag cgccagcgcg tgcagtcccc atgcggtcag
     7081 gggatgcgtc aggtggctcc agaatcggcg ccagcgcggg cgcttcgtgg cggcgcccac
     7141 gcgcggtcgc cacgccgcgg gcagcgccca ggtccaggcc gcgagcgggc ggccgagcac
     7201 cagcagcggc gcggccacga ccatcagcat ctcgtgctgc accatgtgcg ccgagaacag
     7261 cgattcgccc agcggatcca gcggcgagac gagcgcgccg acgagcgcca gccagccggc
     7321 cgtccacgcc gcgacggtgc cggcgcccag gccgtggccc gcgcccgcac gccgccacag
     7381 ccgcgtcgtc ccgagcacgt agagcgccat gctggcggcg agcagcacga cgatccaggg
     7441 ctcgaacgac cacgggaacg cggggctggc gtgatccgcg ttcgcgggca ggtgcgcctc
     7501 gcagcgggcg ctcgcgaacg cggccgccgc gcctgcgagc gcgcgcgcgg gcgtcacgcg
     7561 cgtcggcgcc cgtgcgcgcg ccggcctgct cagtccatgc acggattcaa tacccagacg
     7621 ggcatccaca tgaagagcgc gacgagagcg gaaaggccgc cgacgagcat gccgacgccg
     7681 gcaaggaagt gcgccagcct ggtcggcgtc gtgtcgtcgc gctgggcggc gccggggcga
     7741 tgatccgtga gagcgcgcga cgccagccat tcggcggccg cgagccaggt catcgccagg
     7801 atcgcgacga gcgagacggc cgatacggcg tgcaccggcg cggtcgtctg ctgctcgcac
     7861 gcgtgcttga gcagcgacag gcagatcgac tgctgcgcca gcgccgtgag cggcgccagg
     7921 agcagtgcgg gccaggagcg caggcgtcgc gtcatcgtcg tcacaccacg cggggtgccc
     7981 agtagatcac cgcgtagatc ggcagccagc tcagcacgac gaacacccag tagacggcgt
     8041 tctcgctcac gtcgacgtgg cgcttggcct cgaacggacc gacccgggcc agcgccagca
     8101 gcacggcggt gtcccacgtg tcggtgacca ggtgggtggt gtgcaggccc agcagcagcc
     8161 acaccgccga cgcataggcg tcctcgtacc agcggcagtg cagcgtcgtg aactcgaggc
     8221 cccgcagcac gaggaaggcg agcgcggcga gcaggcacac gcccagccac aggcgcgcgc
     8281 cacgccggtc gaagcgttcc gcggcgcgct tggccaggtg gttgggccac aggctcagca
     8341 gcagcaggac ggtgttgagg ctgccccaga gcagcgaggg cggcggctcg ttcagaggcc
     8401 acaccttgga ctggccgcgc aggtagaagt aggtggcgac ggcgagcgcg aacaccgtcc
     8461 cctcgacggc catcaggccc agcgtgcccc accacatcag gctgcgggta ccgaagccga
     8521 agctgggcag ccgcgcgacg tccagcgccg ggccgtccgc cgctgcgccc ccgccgcgcg
     8581 tgcgccgcga agcgggatcc cagggttcgc tgatgctcat ggccgctgct ccagcgccac
     8641 gctgcggcgc acctcggccc ccttgggcca gaaccagaac gtcatcgcga tggccacggg
     8701 gatgctggcc cacaccacgg cccagggcgt gaagatcgag ccgacgaaca gcacggtggt
     8761 ggcgacggcg ctccagaacg gccatgacgt cggcgccgga aaggtgacgc gcgtctcggg
     8821 cgcggcgtcg agcaccgagg tcaccagcac ctcgcgatcc tcgaccgaca ggcctgccac
     8881 ctggttcggc atgcgcgcgc tctcccacag cggatcgcgt ccgtgcacga cggggatcgc
     8941 cgggaagttg gccgcgtgcg gtggcgacgc ggcggcccat tcgagcgtgc cggcgttcca
     9001 ggggttgtcg ccggcgggtt ctccctgccg gcgcgaacgc cagcaattga gcaggaacag
     9061 cacgacgctg acgccgatcg tcagcgcgcc cagcgtcgcc acgaagttca tgtcgcccca
     9121 gcccatgccc ttggggtacg tccacacgcg tcgcggcatg ccggccaggc ccagcaggtg
     9181 catcgggaag aaggccgcgt tgaagccgat gaagaacagc cagaagttcc agcggcccag
     9241 ccgctcgccc atgcgccgcc cggtgaattt ggggtaccag tagtagaccg cgccgaacag
     9301 cgggaagacg gcgccgccga tcagcacgta gtgcaggtgc gcgacgacga agtaggtgtc
     9361 gtggatctgc aggtccagcg gcaccgcggc gagcatcacg cccgtcagtc cgcccagcac
     9421 caggatgaag aagaagccca ggatgaacag cagtggcgtg cggaacacgg ggcgccccgt
     9481 ccacagcgtg gcgatccagc agaagatctg caccgccgtg gggatcgcga tcagcatgct
     9541 cgcggcggtg aagaagctct tgcccatctc cggcaggttg gtcgcgaaca tgtggtgcac
     9601 ccacaggccg aaggcgagga agccggtggc gatcagcgac agcaccatcg ccgtgtagcc
     9661 gaacaccgga cggcgcgcga aggtggggat gatggtcgag acgaagccca ggcccggcag
     9721 gaagatgaag tagacctccg ggtggccgaa gaaccagaac aggtgctgcc acagcagcgc
     9781 gtcgccgcct tcgcccgggt tgtagaagtg cgtgccgacg agccggtcga ggatcaggtt
     9841 cgtgctcgag aggatgaccg ccggcatcgc gaacatcacc atgaacgcgg tgaccagcat
     9901 gccccagacg aacagcggca tgcggttgag cgtcatgccc ggcgcgcgca gcttgaagac
     9961 ggtggtgatg aggatgatgg cctcgagaac gccggagacc tcggtgaacg tgatcatctg
    10021 cgcccagaag tcgacgcgct tgccgggcga gaaatcgggg ccggcgagcg gcacgtagct
    10081 gaaccagccg gcgtccgggc ccgtgttgca cgcgaaggcg acccacagca tcaggccgcc
    10141 gaacaggtag atccagtagg cgaacgcgtt catgcgcggg aaggcgacgc tgcgcgtgcc
    10201 gatcatcagc ggcaccaggt acaccgacat ggcctgcatc acgggcacgg cgaacaggaa
    10261 catcatcgtc gtgccgtgca tcgtgaagac ctggttgtag aagtccggtc ccatcagctt
    10321 gctttccggc cgcgcgagct gcagccgcat gagcagcgcc agcgcgccgg ccgccacgaa
    10381 gaagccgaag gccgtcacga tgaagcgctt gccgatcgcc ttgtggttga ccgccgagat
    10441 ccagccgccc gggcccggcg ggtcgctcca ggcgcggtcc agcgcgtatt cctgcgccgg
    10501 cgtgggcggg acgtcgttgc cgggggggcg catgaggcct tcgcgggccg gtgtgtcgct
    10561 catgacagcg tccccagcca ggcgacgatg gcgtcgaggt cggcgtcggg catgttggtc
    10621 gcgggcatgt tgacgcccgg cttgaacttg tgcggatcgc ggatccaggc ccgcagcgcc
    10681 tcgggcgtgt tgggcagcgt gccggccgcc agggtgcggc gcgaggcgag gtgcgtcagg
    10741 tcgggtgcgt ggcgcgcgga agccgtggtg ccgcgcaccg cgtggcacat cgcgcaggtg
    10801 gtggagacga acagggtctg tcctttctgc cgcaggggat cgcccggcgg cggtgcgggc
    10861 tggcgctggt gatcgaccca ggccgcgtag cgctcgggcg gttccgcgac gacctcgaac
    10921 gccatccacg cgtgctcggc gccgcagaac tcggcgcact ggccgcggta gatgccgggc
    10981 ttgtcggcgc ggaactggag ggtcgcgtcg cggcccggga tcaggtcctt cttgccggcg
    11041 aggctgggca cccacaggct gtggatgacg tcgtcggcct gcagccgcac caccaccggc
    11101 cgccccaccg gcacatggat ctcgttggcg gtgtcgaacg cctcggacgg cggcccgtcg
    11161 taggtgacct gccaccacca ctggtcggcc gtcaccttga gggtcagcgc gcccttggtc
    11221 gagatgttgg ccagtgcgcg gtccgtcagc acgctcgcga cgatcaggaa cagcagcagc
    11281 accgtggatg cgccgatggc cgtggccacg ctccggtggg ccccgggctc gggcaccgac
    11341 agcgacgcga ggtccggcgg cgcgtccagc cgcccgtgcg gcctcttcag cagggcgatg
    11401 accagagcca ccatcaccgc gacgaacacg agcgtgcaga tggccagcat caggtgccac
    11461 agcgacgcga catgctgcgc ctgcgggccg cccggccgca gcacgtcctg cagcggcgag
    11521 gtcggcgtcc cggcgcgcgc cagcgccgcc tcgagcgcgc acgcggcgag cagcgcggcg
    11581 tcgcggagcc gttcagcgcg tgccatcgtc gatgacctgc ggggtgggaa tttcctgcgt
    11641 gcgacgcgtc tcgggctcgc gcggcgccat gccgtcgctg cggccgggcg cgacgtcgat
    11701 gcgcaactgg cccgagagcg agcgcacgta cgccacgaga tcccacacct ggtcctgcgg
    11761 gatgtgcgcg ccgaacgacg gcatgccggc cggccggccc tgcacgatcg acgcgtagat
    11821 gtcctccggc cgcgaaccgt agatccattt ggcgtcggac agcgccgggc cccagtcgcc
    11881 accgccgccc ggcgcatggc aggcgctgca gttgtaccag cggtaaaggc gcttgccctg
    11941 cgagacggcg taggcgttgc cctcgaccgt gtacggcgtg tcgggctcgg cgggtgcggc
    12001 cggtggcgcg acgggcgccg ggtcgccggg ctggaactcg gtgttgcgcg tgcgcggcgc
    12061 ctgcgccttg ccttggggcg ggtcgaagtc gcgcttttcg cgctcgcacg cggccagcat
    12121 cgcggcgcag ccgaacgcca ggacgagcca tgcggcgcgg cgttcactga agcgagaaga
    12181 catacagcgt ccctcccttc ttcgtgtatt gcggcaggtc gcgcatcgcg ccggcgaagg
    12241 ccttgccggc cgtgcggtcg cgcacgtcga ggtcctgcga cacgaccttg ccggcccagc
    12301 cgccgacgcc cgacaccacc gccacgtact ggtgcccgtc gggcccgcgg tagctgatgg
    12361 gctggccgac gatccccgag tcgaccttgt attgccacag cggccgtccg tcgcgcgcgt
    12421 cgactgcctt gaacc
//
LOCUS       DATCRO010000185         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_193227, whole genome shotgun sequence.
ACCESSION   DATCRO010000185 DATCRO010000000
VERSION     DATCRO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193227"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="yjfF"
                     /locus_tag="VIN75_04995"
     CDS             complement(<1..851)
                     /gene="yjfF"
                     /locus_tag="VIN75_04995"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEY8973553.1"
                     /translation="MNGIKGLRIPPVVASVLLLLAMVTFGSVMYTGFFSAQVFLNLLI
                     DNAFLLVVAVGMTFVILTGGIDLSVGSVVALTTMVSAALVEHRHWPAGLVIPLVLAGG
                     ALFGALQGWLVARFRLQPFLVTLAGMFLARGLCYVISIDSISITDPTFTAIAQAKVTL
                     WTGASISVGSVIALAAFAVGVFVAHGTAFGRNVYAIGGSEQSALLMGVPVARTKVAVY
                     TISGFCSALGGLLLVLYKSSGDPLHAVGMELDAIAAVVIGGTLLTGGSGYVVGTMLGV
                     LVIGLIKT"
     gene            complement(848..1951)
                     /locus_tag="VIN75_05000"
     CDS             complement(848..1951)
                     /locus_tag="VIN75_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8973554.1"
                     /translation="MTTTHDMDTTSPRPAPPTPTPEPAWRRAARHPLAWPLVTLALIL
                     AVNASLNPGLWHLEWRDGHLYGSVVDIVNRAAPLAIVALGLTLVIATRGIDISVGAVV
                     AISGAVAALMIGGDLKIVDGQPVHISHWPLPVALLAALVAAAACGAWNGLLVARAGLQ
                     PIIATLIMMLAGRGAAQLLTGGQIITIYYPPYFVIGNGFFLGVPVAALIALAVWLGMT
                     LLLDRSALGLFIQAIGINPRAARVAGVRARTITICVYAFCGLCAGVAGLIVSSNVKSA
                     DGNNAGVLMELDAILAVTVGGTLLTGGRFTLAGTVIGALIIQTLTSTIYAIGVPPEIN
                     LVVKAVVVFAVMLLQSPEFRRQVRALAFRGGAR"
BASE COUNT          367 a          757 c          728 g          257 t
ORIGIN      
        1 gtcttgatca ggccgatcac cagcacgccg agcatggtgc cgaccacgta gccggaaccg
       61 ccggtcagca gggtgccgcc gattactacc gcggcgatgg cgtccagttc catgcccacc
      121 gcgtgcagcg ggtcgccgga ggacttgtag agcaccagca ggagcccgcc gagcgccgag
      181 cagaacccgc tgatcgtgta gaccgccacc ttggtgcgcg ccaccggcac acccatcagc
      241 agcgcggact gctcgctgcc gccgatcgcg tacacgttgc ggccgaaggc cgtgccatgc
      301 gcgacgaaca cgccgaccgc gaacgccgcc agcgcgatca ccgagcccac cgagatcgag
      361 gcgccggtcc acagcgtcac cttggcctgg gcgatggcgg tgaacgtggg atcggtgatg
      421 ctgatcgagt cgatgctgat cacgtagcac aggccgcgcg cgaggaacat gccggccagc
      481 gtcaccagga acggctgcag ccggaagcgc gccaccagcc agccctgcag cgcgccgaac
      541 agcgcgcccc cggccagcac cagcggaatc acgaggcccg ccggccaatg gcgatgctcc
      601 accagcgcgg ccgacaccat cgtcgtcagc gccaccaccg agcccaccga caggtcgatg
      661 ccgccggtca ggatcacgaa ggtcatgccc accgccacga ccagcaggaa ggcgttgtcg
      721 atcagcaggt tcaggaacac ctgggcgctg aagaagcccg tgtacatcac gctgccgaag
      781 gtcaccatcg ccagcagcag cagcacgctg gcgaccaccg gcggaatgcg cagtcccttg
      841 atcccgttca tcgcgcgcct ccacggaagg ccagggcgcg cacctggcgc cggaactcgg
      901 gggactgcag cagcatcacg gcgaacacga cgacggcctt gacgaccagg ttgatctcgg
      961 gcggcacgcc gatcgcgtag atcgtcgacg tcagcgtctg gatgatcagc gcgccgatca
     1021 ccgtgccggc cagcgtgaac cgcccgccgg tcagcagcgt gccgccgacg gtgacggcca
     1081 ggatcgcgtc cagctccatc agcacgccgg cgttgttgcc gtcggcgctc ttgacgttgg
     1141 agctgacgat cagacccgcc acgcccgcgc acagcccgca gaacgcgtac acgcagatcg
     1201 tgatcgtgcg cgcgcgcacg ccggcgacgc gcgcggcgcg cgggttgatg ccgatcgcct
     1261 ggatgaacag gcccagcgcg ctgcgatcga gcagcagcgt catgcccagc cacaccgcca
     1321 gcgcgatcag cgcggcgacg ggcacgccca ggaagaagcc gttgccgatg acgaagtagg
     1381 gcgggtagta gatcgtgatg atctggccgc ccgtgagcag ctgcgcggcg ccgcggccgg
     1441 ccagcatcat gatgagcgtc gcgatgatgg gctgcaggcc ggcgcgcgcc accagcagcc
     1501 cgttccacgc accgcaggct gcggccgcga ccagcgccgc cagcagcgcg acgggcagcg
     1561 gccagtggct gatgtgcacc ggctggccgt cgacgatctt gaggtcgccg ccgatcatca
     1621 gcgcggccac ggcgccggag atggcgacga ccgcgcccac cgagatgtcg atgccgcgcg
     1681 tggcgatcac cagcgtcagc ccgagcgcga cgatggccag gggagcggcg cggttgacga
     1741 tgtcgaccac gctgccgtac aggtgcccgt cgcgccactc gaggtgccac aggcccgggt
     1801 tcaggctggc gttgacggcc aggatcagcg ccagcgtgac cagcggccac gccagcgggt
     1861 ggcgcgcggc gcgccgccag gccggctcgg gcgttggcgt gggaggcgcg ggccgcggcg
     1921 aggtcgtgtc catgtcgtgc gtcgtggtca ttgcgccggc tcgcagatca ggctcatcac
     1981 ttcggcctcg gtcgcgcccc gcgccagctc gccggccttg cggcgatcgc gcatcacgac
     2041 gatgcggtcg gagatgcgca gcacctcgtc gatctcggac gagatgaaca gcaccgacat
     2101 gccctcgcg
//
LOCUS       DATCRO010000186         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_800755, whole genome shotgun sequence.
ACCESSION   DATCRO010000186 DATCRO010000000
VERSION     DATCRO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800755"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VIN75_05005"
     CDS             complement(<1..370)
                     /locus_tag="VIN75_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8973555.1"
                     /translation="MPAIVSALNTRSEAFAANAARMADKLAEVRRLERQVVDESASRR
                     DKFEQRGQLLPRERVAQLLDRGSGFLELSPLAGLGMHDDDGRKSVQGGGAIVGIGLVS
                     GKRVVISASDSALKGGTVSPM"
     gene            complement(370..1287)
                     /locus_tag="VIN75_05010"
     CDS             complement(370..1287)
                     /locus_tag="VIN75_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8973556.1"
                     /translation="MTTASRDQNDAAIPPSRYRSAFAPGLFAGQRIWVTGGGSGIGRA
                     VAHELASLGAGVVISGRSADKLERVAAEIAEDGGRCTWRAFDIRDDEAVQANVADVIA
                     TQGVVHGLVNNAGGQFPAPMMAISKRGFEAVVASNLTGGFLMMRELFLQSFQAHGGAI
                     VNMTADFRNGMPGMAHSGAARAGMSNLTMTASFEWASCGVRVNAVAPGWIASSGMDTY
                     GGAMKTLIPQLKRHVPLRRMGLEAEVSAAIVFLLSPAAAFITGVTLQIDGGASLGSPL
                     FPSVDHDRSVPFDGFHRAVTPQVLREGEG"
     gene            1495..1794
                     /locus_tag="VIN75_05015"
     CDS             1495..1794
                     /locus_tag="VIN75_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8973557.1"
                     /translation="MDIQTATDQLRAKVGEDCGLGAVLKFDCGSDGAVVIDATRVPNV
                     LSNDADRVADCTVALGVDTLGDLLSGELEPTTGFMMGKFKVSGDMSVALKLQRIV"
     gene            1803..2261
                     /locus_tag="VIN75_05020"
     CDS             1803..2261
                     /locus_tag="VIN75_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8973558.1"
                     /translation="MEPQASKAARGMRAQTADEVVADHRSDDAGQLFGITELCKEFDI
                     SPRALRFYEDKGLLAPRRVNGTRVYTRRDRARLQLILRAKAIGSSLAEIKHYLDLYGT
                     HGEGRAVQMKYVLDKTSKAIAELEQKRAHIDASLAELRIINATVKRGLGK"
     gene            2415..3620
                     /locus_tag="VIN75_05025"
     CDS             2415..3620
                     /locus_tag="VIN75_05025"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8973559.1"
                     /translation="MTEAFIYDHVRTPRGKGKKDGSLHQAAPVWLLRGLLQSLQQRLD
                     LDTSLVDDVVLGCVTPVGEQGADIARTAVLDAGWDERVAGVTLSRFCASGLEAANLAA
                     AKVASGFEDFVVAGGIESMSRWTMGSDGGAWFMDPRINQGIGFIPQGVSADLIATLEG
                     FSRHELDSYALQSHRRAAAARAAGHFRKSVVPVHDIAGLLMLAEDETIRANATLEAMA
                     RLEPSFAAMGAMGFDATALRKYTTVEKITHVHHAGNASGIVDGAALMLLGSRGAGERA
                     GLRPRARIRSAAVTGSEPTIMLTGPTPACRKALAKAGLKAGDIDLWEINEAFAVVPMK
                     TARDLGVSLDRVNVNGGAIALGHPLGATGCIILGTLLDELERRDQALGCATLCVGGGM
                     GIATIIERI"
     gene            3624..>5757
                     /locus_tag="VIN75_05030"
     CDS             3624..>5757
                     /locus_tag="VIN75_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8973560.1"
                     /translation="MSDVLDVMVGDDGIAVATMDSPGRSMNVLDDELAQPLAALVERL
                     ETDASVKGLVLRSGKKDFLAGADIDRLRALASAQEAFDESMRLKKALRRLELCGKPVV
                     AAINGKCLGGGLEIALACHHRIVLDDGKARLGLPEVKLGLLPGGGGTQRMPRLVGMQQ
                     ALQWMGEGADIRAAQALESGLVGELATSADDMLAKAKAWIAANPSPRQPWDTPKFRYP
                     GGDSRTPAAAQMFSVAPSMASAKSFGNYPAVTHILSCVFEGGVVGFDAGLELESRYFA
                     ACAMSQESRNLIGTLWYQLQAIERGASRPAGHAPRSVRRLGILGAGMMGAGIAYVAAK
                     AGIEVMLLDTSPDNAVRGRQYSQDLLDKAVRKGRTTPGQRDALLARITPTVKFEDLAG
                     CDLVVEAVFEDRAIKADVTRRTEALLSPDAVFASNTSTLPITSLAQASVRPAQFIGLH
                     FFSPVDRMPLVEIIVGRQTDDATLALAFDFVRQIGKTPIVVNDSRGFYTSRVFATYVM
                     EGLAMLREGVHPRHIEAAGLQAGMPMPPLALQDEVSLTLALHVADQTRKDLAAEGQAY
                     VEHPGEAVLRQMCEIGRIGRKANLGFYDWTDAGRSLWPGLAQMFPVAAQQPAQRELID
                     RLMFVQANEAARCVEEGVLRSVADANVGSIFGWGFAPFHGGVLQFINAMGARRFVQRL
                     RELARAHGARFEPAGVVVNLAERGGVFED"
BASE COUNT          861 a         2049 c         2034 g          813 t
ORIGIN      
        1 ccatcggcga gaccgtgccg cccttgagcg cgctgtcgct cgcggagatc acgacccgct
       61 tgcccgacac cagcccgatg cccacgatgg cgccgccgcc ctgcacgctc ttcctgccgt
      121 cgtcgtcgtg catgcccagc ccggccagcg gcgacagttc caggaagccg ctgccgcgat
      181 cgagcagctg cgcgacgcgc tcgcgcggca gcagctggcc gcgctgctcg aacttgtcgc
      241 gcctggacgc gctttcgtcg acgacctggc gctcgagccg gcgcacctcg gcgagcttgt
      301 cggccatgcg cgccgcattg gccgcgaacg cctcggacct cgtgttcagc gcggagacga
      361 tcgccggcat cagccctccc cttcgcgcag cacctgcggc gtcacggcgc gatggaagcc
      421 gtcgaacggc accgagcggt cgtggtccac cgacgggaac agcggcgagc ccagcgacgc
      481 gccgccgtcg atctgcagcg tgacgccggt gatgaacgcg gcggccggcg acagcaggaa
      541 cacgatggcc gcgctcacct cggcctcgag ccccatgcgg cgcagcggca cgtggcgctt
      601 gagctggggg atcagcgtct tcatcgcgcc gccgtaggtg tccatgccgc tggaggcgat
      661 ccagcccggc gcgacggcgt tgacgcgcac gccgcaggag gcccactcga acgacgccgt
      721 catcgtgagg ttgctcatgc ccgcgcgcgc cgcgcccgag tgcgccatcc cgggcatgcc
      781 gttgcggaag tcggccgtca tgttgacgat ggcgccgccg tgcgcctgga agctctgcag
      841 gaagagctcg cgcatcatca ggaagccgcc ggtcaggttg ctcgcgacga cggcctcgaa
      901 gccgcgcttg ctgatggcca tcatcggcgc cggaaactgg ccgccggcgt tgttgacgag
      961 gccgtggacg acgccttgcg tggcgatgac atcggcgacg ttggcctgga cggcctcgtc
     1021 gtcgcggatg tcgaacgcgc gccaggtgca gcggccgccg tcctcggcga tctcggccgc
     1081 gacgcgctcg agcttgtcgg cgctgcggcc gctgatgacg acgccggcgc ccagcgaggc
     1141 cagctcgtgg gccacggcac ggccgatccc cgagccgccg ccggtcaccc agatgcgctg
     1201 gccggcgaac agccccggcg cgaacgcgct gcgatagcgc gaaggaggta ttgcggcgtc
     1261 gttctgatcg cgagaggctg tcgtcatgtg gacacgatcg ttaccggatg gacgggatgg
     1321 agcccgcggt gcttgccgca ttaggcttca gggtgacgtt aacgtcaacc tcacctcgcg
     1381 tcgggaattt cactgatgcc gacgtcactt gcaggcccta gcatggcgtc gaccgcgtgg
     1441 cgcgccatcg cgcgccgtcg cttgccatcc acttccgccc cgggaaccca tcgcatggac
     1501 atccagaccg ccaccgatca actgcgcgcc aaggtcggcg aggactgcgg cctcggtgcc
     1561 gtgctcaagt tcgactgcgg ctccgacggc gccgtcgtca tcgacgccac cagggtgccc
     1621 aacgtgctgt ccaacgacgc cgaccgcgtg gccgactgca ccgtcgcgct gggcgtggac
     1681 accctcggcg acctcttgtc gggcgagctg gagcccacca caggcttcat gatgggcaag
     1741 ttcaaggtct cgggcgacat gagcgtcgcg ctgaagctcc aacgcatcgt ctgagcgccg
     1801 ccgtggaacc gcaggcatcg aaagccgcgc gcggcatgcg cgcgcagacc gccgacgagg
     1861 tggtggccga ccatcgcagc gacgacgccg gccagctgtt cggcatcacc gagctgtgca
     1921 aggagttcga catctcgccg cgcgcgctgc gcttctacga ggacaagggc ctgctcgcgc
     1981 cgcgccgcgt caacgggacg cgcgtctaca cccggcgcga ccgcgcgcga ctgcagctga
     2041 tcctgcgcgc caaggccatc ggctcgtcgc tggccgagat caagcattac ctggacctgt
     2101 acggcaccca cggcgagggc cgcgcggtgc agatgaagta cgtgctcgac aagacctcca
     2161 aggcgatcgc cgagctcgag cagaagcgcg cgcacatcga tgcgtcgttg gcggagctgc
     2221 gcatcatcaa cgccaccgtc aagcgcggcc tggggaagta ggacacgcca tgacggcacc
     2281 acccgcaggg gtctggcatc tcctcggttc gcgccgggcc tccatcgaca gcggcaacgc
     2341 cggccgcgaa ccgaggcgaa tgccagaccc cctccggcca gaccccgcgc taccccggtt
     2401 ctgaaagcag acacatgacc gaagcattca tctacgacca cgtccgcacc ccgcgcggca
     2461 agggcaagaa ggacggcagc ctgcaccagg ccgcaccggt gtggctgctg cgcgggctgc
     2521 tgcagtcgct gcagcagcgc ctggacctgg acacgtcgct cgtcgacgac gtcgtcctcg
     2581 gctgcgtcac gccggtgggc gagcaaggcg ccgacatcgc gcgcaccgcg gtgctggacg
     2641 ccggctggga cgagcgcgtc gccggcgtca cgctgtcacg cttctgcgcg tcgggcctgg
     2701 aggcggccaa cctcgcggcg gccaaggtcg ccagcggctt cgaggacttc gtcgtggccg
     2761 ggggcatcga gtcgatgtcg cgctggacga tgggatcgga cggtggcgcg tggttcatgg
     2821 atccgcgcat caaccagggc atcggcttca tcccgcaggg cgtcagcgcc gacctgatcg
     2881 ccacgctgga aggcttctcg cgccacgagc tcgattctta cgcgctgcag tcgcatcgac
     2941 gggccgcggc cgcgcgcgcg gcgggccact tccgcaagtc ggtcgttccc gtgcacgaca
     3001 tcgccggcct gctgatgctg gccgaggacg agacgatccg cgccaacgcc acgctggagg
     3061 ccatggccag gctggagccg tcgttcgccg cgatgggcgc gatgggcttc gacgccaccg
     3121 cgctgcgcaa gtacacgacg gtcgagaaga tcacgcacgt gcaccatgcc ggcaacgcgt
     3181 cgggcatcgt cgacggcgcc gcgctgatgc tgctgggctc gcgcggcgcg ggcgagcgcg
     3241 cgggcctgcg gccgcgggca cgcatccgca gcgccgccgt caccggctcc gagcccacca
     3301 tcatgctcac cggtccgacg ccggcctgcc gcaaggcgct ggccaaggcc gggctgaagg
     3361 cgggcgacat cgacctgtgg gagatcaacg aggcgttcgc cgtggtgccg atgaagacgg
     3421 cgcgcgacct gggcgtgtcg ttggaccgcg tcaacgtcaa cggcggtgcg atcgcgctcg
     3481 gccacccgct gggcgccacc ggctgcatca tcctcggcac gctgctcgac gagctggaac
     3541 gccgcgacca ggcgctcggc tgcgccaccc tgtgcgtggg cggtggcatg ggcatcgcca
     3601 cgatcatcga gaggatctga aagatgagcg acgtactcga cgtgatggtg ggcgacgacg
     3661 gcatcgccgt cgccacgatg gattccccgg gccgttcgat gaacgtgctc gacgacgagc
     3721 tcgcgcagcc gctggccgcg ctggtcgagc gcctggagac cgacgcgtcg gtcaagggcc
     3781 tggtgctgcg ctcgggcaag aaggacttcc ttgcgggcgc cgacatcgac cgcttgcgcg
     3841 cgctggcgtc ggcgcaggag gccttcgacg aatcgatgcg cctgaagaag gcgctgcgcc
     3901 gcctcgagct gtgcggcaag ccggtcgtcg cggccatcaa cggcaagtgc ctgggcggcg
     3961 gcctggagat cgcgctcgcc tgccaccacc gcatcgtgct ggacgacggc aaggcgcggc
     4021 tgggcctgcc cgaagtcaag ctgggcctgc tgcccggcgg cggcggcacg cagcgcatgc
     4081 cgcgcctggt gggcatgcag caggcgttgc agtggatggg cgaaggcgcc gacatccgcg
     4141 cggcgcaggc gctcgagtcg ggcctggtcg gcgagctcgc gacctcggcc gacgacatgc
     4201 tcgccaaggc caaggcctgg atcgccgcga acccgtcgcc caggcagcca tgggacacgc
     4261 ccaagttccg ctaccccggc ggagactcgc gcacgccggc ggcggcgcag atgttctccg
     4321 tcgcgccgtc catggcgtcg gccaagagct tcggcaacta tccggcggtc acgcacatcc
     4381 tgtcgtgcgt gttcgagggc ggcgtcgtcg gcttcgacgc gggtctcgag ctcgagtcgc
     4441 gctacttcgc ggcctgcgcg atgtcgcagg aatcgaggaa cctgatcggc acgctgtggt
     4501 accagctgca ggcgatcgag cgcggcgcct cgcggcccgc gggccacgcg ccacgcagcg
     4561 tcaggcgcct gggcatcctg ggcgccggca tgatgggcgc gggcatcgcc tacgtcgccg
     4621 ccaaggccgg catcgaggtg atgctgctgg acacctcgcc cgacaacgcc gtgcgcggcc
     4681 gccagtactc gcaggacctg ctggacaagg ccgtcaggaa ggggcggacg acgcccgggc
     4741 agcgcgacgc cctgctcgcc cgcatcacgc cgaccgtgaa gttcgaggac ctggccggct
     4801 gcgacctcgt cgtcgaggcc gtgttcgagg atcgcgccat caaggccgac gtcacgcgcc
     4861 gcaccgaggc gctgttgtcg cccgacgccg tgttcgcgtc gaacacgtcc acgctgccca
     4921 tcaccagcct cgcgcaggcg agcgtgcggc cggcgcagtt catcggcctg cacttcttct
     4981 cgccggtgga caggatgccg ctcgtcgaga tcatcgtggg ccggcagacc gacgacgcca
     5041 cgctggcgct ggcgttcgac ttcgtgcgcc agatcggcaa gacgcccatc gtcgtcaacg
     5101 actcgcgcgg cttctacacg agccgcgtgt tcgccaccta cgtgatggaa ggcctggcga
     5161 tgctccggga aggcgtgcac ccgcgccaca tcgaggccgc gggcctgcag gcgggcatgc
     5221 ccatgccgcc gctggcgctg caggacgagg tgtcgctgac gctcgcgctg cacgtggccg
     5281 accagacccg caaggacctc gccgccgagg gccaggccta cgtcgagcat ccgggcgagg
     5341 cggtgctgcg ccagatgtgc gagatcggcc gcatcggccg caaggccaac ctcggcttct
     5401 acgactggac ggacgcgggc cgcagcctgt ggccaggtct ggcgcagatg ttcccggtgg
     5461 ccgcgcagca gcccgcgcag cgggaactga tcgaccgcct gatgttcgtg caggccaacg
     5521 aggccgcgcg ctgcgtcgag gaaggcgtgc tgcgctcggt ggccgacgcc aacgtcggct
     5581 cgatcttcgg ctggggcttc gcgcccttcc acggcggcgt gctgcagttc atcaacgcga
     5641 tgggtgcgcg gcgcttcgtg cagcgcttgc gcgagctcgc ccgggcgcac ggcgcccgct
     5701 tcgagccggc gggcgtggtg gtgaacctcg ccgagcgcgg cggcgtattc gaggact
//
LOCUS       DATCRO010000187         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_440796, whole genome shotgun sequence.
ACCESSION   DATCRO010000187 DATCRO010000000
VERSION     DATCRO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440796"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VIN75_05035"
     CDS             complement(<1..703)
                     /locus_tag="VIN75_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8973561.1"
                     /translation="MSGAGRPLRVVLFGDGESPHLLKWARALAPRVELFVASSRGLAP
                     ALAALLPADRTLLLSHDTKFAGGNAALLKALPRLVRWLRGVDADWVNAHYLTSHGTLA
                     WLARKCGIGARLTASAWGSDILVTPGQSALLRWVTGRVLRDADVCTSDSEYMARRMRE
                     LGAREVMTFPFGLDALPPEPAGKEPRLFFANRGLEDIYDPMRVLSLFAGAAAAWPDAR
                     LVVANGGSLREALEAR"
     gene            complement(700..1320)
                     /locus_tag="VIN75_05040"
     CDS             complement(700..1320)
                     /locus_tag="VIN75_05040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8973562.1"
                     /translation="MTLRASFNRLRRRWLRRWVWQPVFGEQQGTRLLPHTRISSASVI
                     EHEDKLILGDNVFIGAFNFIEASGGITIEEGVQVTSHCAIVTHSSHRSQRLLGPGYVT
                     WPVPAGGARPGWIAGPIHIGAYSFVGPHSLIEANTHLGRGTLVCAGSFVRGTYPDYAI
                     LEGRPARVVGDSRRADEKALVRYPELRVLYEAWTTAPEPIGLEGPK"
     gene            complement(1317..>2086)
                     /locus_tag="VIN75_05045"
     CDS             complement(1317..>2086)
                     /locus_tag="VIN75_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY8973563.1"
                     /translation="LIVAPIAAAAAAAALLRLPLNDEADPAPERWRALARRFRDFPLL
                     NTPHAFMGALQDTAAIALLTAWQGPVAAGFWGFALRYLKAPATLVGGAVSQTLYPRLA
                     AHGASAGQDADAHAPLRVTREGRAAVRRVMAGLAAIAAPLVLLLWAVGPWAFEWLFGA
                     QWRGAGELSRALAVYIGMHFVAAPLAVVTLAWGAQAWALRLALVGQVAFVGALAIGLR
                     MGGLAGAGWAVSIAMTLYFGYYFLRLATWPLTPLEQR"
BASE COUNT          306 a          782 c          774 g          224 t
ORIGIN      
        1 cccgcgcctc gagcgcctcg cgcagcgagc cgccgttggc cacgaccagg cgcgcgtcgg
       61 gccacgcggc cgcggcgccg gcgaacagcg acagcacgcg catcgggtcg tagatgtcct
      121 cgagcccgcg gttggcgaag aacagccgcg gctccttgcc cgcaggctcg ggcggcagcg
      181 cgtcgaggcc gaacgggaac gtcatgacct cgcgcgcgcc gagctcacgc atgcgacggg
      241 ccatgtattc ggagtcgctc gtgcagacgt cggcgtcgcg cagcacgcgc ccggtgaccc
      301 agcgcagcag cgcgctctgg ccgggcgtga cgaggatgtc cgagccccag gccgacgccg
      361 tcagccgcgc gccgatgccg cacttgcgcg ccagccaggc gagcgtgccg tgcgaggtga
      421 ggtagtgggc gttgacccag tcggcgtcga cgccgcgcag ccagcgcacc aggcgcggca
      481 gcgccttcag cagcgcggcg ttgccgccgg cgaacttcgt gtcgtgcgac agcagcagcg
      541 tgcggtcggc cggcagcagc gcggcgagcg ccggcgcgag gccgcggctg gacgcgacga
      601 acagctcgac ccgcggcgcc agcgcccgcg cccacttgag caggtgcggg ctctcgccgt
      661 cgccgaacag cacgacccgc agcgggcggc cagccccgct cacttgggcc cttcgaggcc
      721 gatcggctcg ggcgccgtcg tccaggcctc gtacagcacc cgcaactcgg gatagcgcac
      781 cagcgccttc tcgtcggcgc gccggctgtc gcccaccacc cgggccgggc ggccctcgag
      841 gatcgcgtag tccggatagg tgccgcgcac gaagctgccc gcgcacacga gggtgccgcg
      901 gccgaggtgc gtgttggcct cgatcagcga atgcggcccg acgaagctgt acgcgccgat
      961 gtggatcggg ccggcgatcc agccgggacg cgcgccgccg gcgggcaccg gccaggtgac
     1021 atagcccggg cccagcaggc gctgcgagcg gtgcgagctg tgcgtgacga tcgcgcagtg
     1081 gctggtgacc tgcacgccct cctcgatcgt gatgccgccg ctggcctcga tgaagttgaa
     1141 cgcgccgatg aacacgttgt cgcccaggat cagcttgtcc tcgtgctcga tcacgctcgc
     1201 gctcgagatg cgcgtgtgcg gcagcaggcg cgtgccctgc tgctcgccga acaccggctg
     1261 ccagacccag cgccgcagcc agcggcggcg caggcggttg aaggaggcgc gcagggtcat
     1321 cgctgttcca gtggagtgag cggccaggtg gccagccgca ggaagtagta gccgaaatac
     1381 agtgtcatcg cgatcgacac ggcccagccg gcgccggcca ggccgcccat tctcaggccg
     1441 atcgccagcg cgccgacgaa cgccacctgc cccaccagcg cgagccgcag cgcccaggcc
     1501 tgcgcgcccc aggcgagcgt gaccacggcc agcggcgcgg ccacgaagtg catgccgatg
     1561 tagacggcca gcgcgcgcga cagctcgccg gcgccgcgcc actgcgcgcc gaacagccat
     1621 tcgaacgccc acgggccgac ggcccacagc agcagcacca gcggcgcggc gatggcggcc
     1681 aggccggcca tcacgcgccg cacggccgcg cgtccctcgc gcgtcacgcg caggggcgcg
     1741 tgcgcgtccg cgtcctggcc ggcggacgcg ccatgcgcgg cgagccgcgg atagagcgtc
     1801 tgcgacacgg cgccgcccac cagcgtggcc ggcgccttca ggtagcgcag cgcgaagccc
     1861 cagaagccgg ccgcgaccgg gccctgccag gcggtcagca gcgcgatggc ggcggtgtcc
     1921 tgcagcgcgc ccatgaaggc atgcggcgtg ttgagcagag gaaagtcgcg gaagcgccgc
     1981 gcgagcgcgc gccagcgctc cggcgcagga tcggcctcgt cattcagcgg cagccgcagc
     2041 agcgcggcgg ccgccgcggc ggcggcgatc ggcgccacga tgagtc
//
LOCUS       DATCRO010000188         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_530818, whole genome shotgun sequence.
ACCESSION   DATCRO010000188 DATCRO010000000
VERSION     DATCRO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530818"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /gene="uvrA"
                     /locus_tag="VIN75_05050"
     CDS             <1..1789
                     /gene="uvrA"
                     /locus_tag="VIN75_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8973564.1"
                     /translation="TVVKRAHPFEGILPNFERRFRETDSAAVREDLARYQAARACPAC
                     HGTRLRREARNVFLVGAGTERKPIYSIEHATLSDCLGYFEQLKLEGARADIADKVVRE
                     IRSRLTFLNNVGLNYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLH
                     QRDNDRLIDTLRHLRDLGNSVLVVEHDEDMIRASEFCIDMGPGAGRHGGQVIAQGTPD
                     EVAANPESLTGKYLSRALVIPVPDERHEWRDEPEDGDPKDTPRLRVRGARGNNLKDVT
                     VDIPVGLLTCVTGVSGSGKSTLVNDTLYNAVARELYGSHAEAAPHEALEGLEAFDKVI
                     NVDQSPIGRTPRSNPATYTGLFTPIRELFAEVPAARERGYGPGRFSFNVSGGRCESCQ
                     GDGMLKVEMHFLPDVYVPCDICHGKRYNRETLEVLYKGKHIAQVLDLTVEDALEFFSA
                     VPNIARKLQTLLDVGLGYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEPT
                     TGLHFADIQLLLTVLHQLRDAGNTIVVIEHNLDVIKTADWVIDMGPEGGAGGGTVVVE
                     GTPEDVAACAASHTGRYLAPYLEESRRRA"
     gene            complement(1817..>2201)
                     /locus_tag="VIN75_05055"
     CDS             complement(1817..>2201)
                     /locus_tag="VIN75_05055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973565.1"
                     /translation="ADIDRFGRVNDAHGREFGDQVIKAVAEALRALVPAGATLARMDG
                     QAFGLLVPGLDAAAALLLADRLRAHIGSARIHRPDREDAERVTLSVGLARARSGEAPQ
                     ALLGRAGAALRQAKDAGRDRVVSGG"
BASE COUNT          342 a          821 c          764 g          274 t
ORIGIN      
        1 gacggtggtc aagcgcgcgc atccgttcga gggcatcctg cccaacttcg agcggcgctt
       61 ccgcgagacc gactccgccg ccgtgcgcga ggacctggcg cgctaccagg ccgcgcgcgc
      121 ctgcccggcc tgccacggca cgcgcctgcg ccgcgaggcg cgcaacgtgt tcctggtggg
      181 cgccggcacc gagcgcaagc cgatctacag catcgagcac gccacgctgt ccgactgcct
      241 cggctacttc gagcagctca agctcgaggg cgcgcgggcc gacatcgccg acaaggtggt
      301 gcgcgagatc cgcagccgtc tcacgttcct gaacaacgtc ggcctgaact acctgagcct
      361 cgaccgcagc gccgacacgc tgtccggcgg cgaggcacag cgcatccggc tcgcgtcgca
      421 gatcggctcg ggcctgacgg gcgtgatgta cgtgctggac gagccctcca tcgggctgca
      481 ccagcgcgac aacgaccgcc tgatcgacac gctgcgccac ctgcgcgacc tcggcaactc
      541 ggtgctggtg gtcgagcacg acgaggacat gatccgcgcc tcggagttct gcatcgacat
      601 gggcccgggc gccggccgac acggcggcca ggtgatcgcg cagggcacgc ccgacgaggt
      661 ggcggccaac cccgagtcgc tgaccggcaa gtacctgtcg cgcgcgctcg tcatcccggt
      721 gcccgacgaa cgccacgaat ggcgcgacga gcccgaggac ggcgacccga aggacacgcc
      781 gcgcctgcgc gtgcgcggcg cgcgcggcaa caacctcaag gacgtgacgg tggacatccc
      841 cgtcggcctg ctcacctgcg tgacgggcgt ctcgggctcg ggcaagtcca cgctggtcaa
      901 cgacacgctg tacaacgcgg tcgcccgcga gctgtacggc agccacgccg aggccgcgcc
      961 gcacgaggcg ctggaagggc tggaggcgtt cgacaaggtc atcaacgtcg accagagccc
     1021 gatcggccgc acgccgcgct ccaacccggc cacctacacc gggctgttca cgccgatccg
     1081 cgagctattc gccgaggtgc cggcggcgcg cgagcgaggc tacggaccgg ggcgcttcag
     1141 cttcaacgtg tcgggcggcc gctgcgagag ctgccagggc gacggcatgc tgaaggtgga
     1201 gatgcacttc ctgcccgacg tctacgtccc ctgcgacatc tgccatggca agcgctacaa
     1261 ccgcgagacg ctggaggtgc tctacaaggg caagcacatc gcgcaggtgc tcgatctcac
     1321 cgtggaggac gccctcgagt tcttctccgc cgtgcccaac atcgcgcgca agctgcagac
     1381 gctgctggac gtgggcctgg gctacatcac gctgggccag agcgcgacga cgctgtccgg
     1441 cggcgaggcg cagcgcgtca agctggcgct cgagctgagc aagcgcgaca cgggccgcac
     1501 gctctacatc ctcgacgagc cgaccaccgg cctgcacttc gccgacatcc agctgctgct
     1561 gaccgtgctg caccagctgc gcgacgccgg caacaccatc gtcgtcatcg agcacaacct
     1621 ggacgtcatc aagacggccg actgggtgat cgacatgggc cccgagggcg gcgccggcgg
     1681 cggcaccgtc gtggtggaag gcacgcccga ggatgtcgcg gcgtgcgccg ccagccacac
     1741 cgggcgctac ctggcgccgt acctggaaga gtcgcgccgg cgcgcgtgac gcgcgcggcg
     1801 cgcaggccgc gatcgatcag cccccgctga cgacgcggtc gcggccggcg tccttggcct
     1861 ggcgcagtgc ggcgccggcg cgccccagca gcgcctgcgg cgcctcgccg ctgcgggcgc
     1921 gagccagtcc gaccgacaac gtcacgcgct ccgcgtcctc gcgatcgggc cggtggatgc
     1981 gcgcgctgcc gatgtgcgcg cgcaggcggt cggccagcaa cagcgcggcg gcagcgtcca
     2041 ggcccggcac cagcaggccg aacgcctggc cgtccatgcg cgccagcgtc gcgcccgccg
     2101 gcaccagcgc gcgcagcgcc tcggccaccg ccttgatcac ctggtcgccg aattcgcgtc
     2161 cgtgggcgtc gttgacccgg ccgaagcggt cgatgtcggc c
//
LOCUS       DATCRO010000189         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_845950, whole genome shotgun sequence.
ACCESSION   DATCRO010000189 DATCRO010000000
VERSION     DATCRO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845950"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VIN75_05060"
     CDS             <1..655
                     /locus_tag="VIN75_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8973566.1"
                     /translation="STIVFLVRKGNPKGIHDWSDIAKPGVGVVVANPKTSGGARWAYL
                     AAYGWALKQPGASDATARDYIRKVYENVPVLDSGARGSTVTFAQRGVGDVLLAWENEA
                     YLSLKEFGADKFDVVYPPSSILAEPPVAIVDKVVDRKGTRAVAQAYLEYLYTPEAQEI
                     EAKNYYRPIDPTVAAKYAGKFPKLKLFSIDDTFGGWTNAQKTHFADGGVFDQIYTKK"
     gene            907..>5879
                     /locus_tag="VIN75_05065"
     CDS             907..>5879
                     /locus_tag="VIN75_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY8973567.1"
                     /translation="MSPLLAHADATLKTMSPRGEVALVRQVRATFSESMVKFGDPRLP
                     APLDVACTPGTAQGGTGRWVDDKTWVFDFAKDLPPGARCSVTPHPGIRSVAGAAIAAS
                     AKFDFGTGGPAIVRAYPTPSDWAKVEEEQVFALLLNGPATADSIQRYAYCEFAGVGER
                     VPVKVITGATRDAILQAVNLVPQKERAVTLQCARPVAPESRMSLVWGKGIATATGVAS
                     TDDRRLAYTVRPAFTANFTCERVNSRSDCLPMRPLRLEFSSPVPRKIAEKVALVGPDG
                     THKPDVEQHRGEASDALLEMHQSGFQKFIYFFGRNKGSVAGDASEEGVTAVQWMAQLP
                     EKADLRIELPPDFADDSGRRLENAAQFPLKTRTAEAPPLAKFPAATFGILELNADPVL
                     PLTVRKIEGQLGVRAVTLGGAPARDLTLTDDAAIIDWYARLARYNETSLARSTVESEL
                     GVRLPVPPKKAPARQAKDHGRTKSQEEQDEDADESDVNAQDPNAVQTRTVSLLAKAPG
                     VHLVKLPTESQTEPHPFEVIGIPLPQPGYHVVEIESPRLGEALLDRKAPMYVRTGVLV
                     TNLAVHFKWAPVNSGAWVTTLDKGQPVQGATVQVSDCRGEKVWSGRTDATGFARIDQP
                     LPRMRWEYCSHAGNERWAGQTGRPEGYFVSARKTDAQGRHDMAFVWSTWQEGIEAWRF
                     NLNSQGRGEEVSHPLFHTVMDRTLLRAGQTISMKSHARRELLTGLALVDAGDLPTTLR
                     IEHEGSDDHYEFPLTWRAGRYAETAWKIPEDAKLGVYDVTLIREGRISVSTGSFRVEE
                     FRLPAMVGRLVPPPGPQVDPRELTMDVMVNYGNGGGAAGLPLKVSAQERDIDLSRAIP
                     MQRWPGFHFEPPKAPSTDQGARDPDGAMFSEEYVDEDDADRMVTQRDPNSKLVADKLP
                     VTLDKSGAGKVTLPKLPPVTAPRELLVQATYADPNGEVQTLSQTLPLWPSGVVLGVRT
                     DDWVSVHQKLSTQVVALDTKGRPQAGVSVSVRAVVHRQISARKRLVGGFYAYDNRNVD
                     EDLGEVCSGSSDARGLVLCEAELSTSGQVELVAQAKDAQGHLARAASSVWVTRAGEVW
                     FGSANDDRMDVLPEQRAYEPGQVAKFQVRSPFRHATALVAIERNGIVETREVQLDGRD
                     PTIELPVKAEWAPNVFVSVLAVRGRVREVPWYSLFSWGWKSPVEWWHAWRNEGQDWQA
                     PTAMVDLAKPAFRFGIAEIEVGDAAHRLKVDVTPDKATYPIRATSQVRIKVTLPDGRP
                     APAGTEVTLAAVDEALLSLKANDSWDLFDAMIRRRDYGVETATAQMQIIGKRHFGKKA
                     APPGGGGGQFPTRELFDTLLLWNPSVVLDAKGEAVVGVPLNDSLTSFRIVAVADVVQG
                     AQSALFGTGQATIAATQDLQIVSGVPPLVREGDHYKAMFTLRNTSKAAMNASLAASAG
                     AQSLPAQTVAIAAGAAADVSWDVDVPFNVKSLAWTVAADAGAAHDRMKFAQKVAEAVP
                     VTVQQATLMQLDKTLRVPIAPPVNALPDGTGKLRGGIEVALKPRLGDGLPGVREYFQW
                     YPWDCFEQRASVAIGLRDTTRWHALVGQIALYQDEDGLLNYFPNGRPHDGSDSLTAYV
                     LAISDEATRLGYDFALPDDAKASLQRGLIGFVEGHVNRDFWRPAFLK"
BASE COUNT          927 a         2021 c         2139 g          792 t
ORIGIN      
        1 gtcgacgatc gtgttcctgg tgcgcaaggg caacccgaag ggcatccacg actggtccga
       61 catcgccaag cccggggtgg gcgtggtcgt ggccaacccg aagacctcgg gcggcgcgcg
      121 ctgggcctac ctggcggcct acggctgggc gctgaagcag ccgggtgcga gcgatgccac
      181 ggcgcgcgac tacatccgca aggtctacga gaacgtgccg gtgctcgact ccggcgcgcg
      241 cggatcgacc gtcacgttcg cgcagcgcgg cgtcggcgac gtgctgctgg cgtgggagaa
      301 cgaggcctac ctgtcgctca aggaattcgg cgccgacaag ttcgacgtcg tctacccgcc
      361 gtccagcatc ctcgccgagc cgccggtggc gatcgtggac aaggtggtcg accgcaaggg
      421 cacgcgcgcc gtggcccagg cctacctcga gtacctgtac acgcccgagg cgcaggagat
      481 cgaggcgaag aactactacc gcccgatcga tccgaccgtg gccgccaagt acgcgggcaa
      541 gttcccgaag ctgaagctgt tctcgatcga cgacaccttc ggcggctgga ccaacgccca
      601 gaagacccac ttcgccgacg gcggcgtgtt cgaccagatc tatacgaaga agtaaatccc
      661 ccgacgaccg cggcgcgcga cgccgccagg gtcgtcgcgc gtttcacgag ggccgtgcgc
      721 gatgcgcgcg gctcctgcct ttcgtgagac cgcgttccgg tgcgtgagac ttcgctcacg
      781 acgccccgtt gaccgccgat ccaccgcata gactccgtcc ccaagccgcc cgacgcacgg
      841 gtgcagggga atcagaacat gtggtggtgg tggagccgga aactcacgct cggtatggtc
      901 gcgtcgttgt cgccgttgct ggcgcatgcc gatgcgacgt tgaagacgat gtcgccgcgc
      961 ggcgaggtcg cgctggtgcg ccaggtgcgc gccacgttca gcgagtcgat ggtcaagttc
     1021 ggcgacccgc ggctgcccgc gccgctggac gtcgcctgca cgcccggtac cgcgcaaggc
     1081 ggcacggggc gctgggtcga cgacaagacc tgggtcttcg acttcgccaa ggacctgccg
     1141 cccggcgcgc gctgcagcgt cacgccgcat ccgggcatca ggtcggtggc cggcgcggcc
     1201 atcgccgcga gcgcgaagtt cgacttcggc accggcgggc ccgccatcgt gcgggcctac
     1261 ccgacgccca gcgactgggc caaggtcgag gaggagcagg tcttcgcgct gctgctcaac
     1321 ggtccggcca cggccgacag catccagcgc tacgcgtact gcgagttcgc cggcgtcggc
     1381 gagcgggtgc cggtcaaggt catcaccggc gcgacgcgcg acgcgatcct ccaggccgtc
     1441 aacctcgtgc cgcagaagga gcgcgccgtg acgctgcaat gcgcgcggcc ggtggcgccc
     1501 gagtccagga tgtcgctggt gtggggcaag ggcatcgcca ccgccacggg ggtcgcgtcg
     1561 accgacgatc ggcgcctcgc ctacaccgtg cggcccgcgt tcaccgcgaa cttcacctgc
     1621 gagcgcgtga actcgcgctc ggactgcctg ccgatgcggc cgctgcggct cgagttctcg
     1681 tcgccggtgc cgcgcaagat cgccgagaag gtggcgctcg tcgggccgga cggcacgcac
     1741 aagcccgacg tcgaacagca tcgcggcgag gcctccgacg cattgctgga gatgcaccag
     1801 tcgggcttcc agaagttcat ctacttcttc ggccgcaaca agggcagcgt ggcgggcgac
     1861 gcgtcggagg agggcgtcac cgcggtgcaa tggatggccc agctgcccga gaaggccgac
     1921 ctgcgcatcg aactgccgcc tgacttcgcc gacgactccg gccgcaggct ggagaacgcc
     1981 gcgcaattcc cgctgaagac ccgtaccgcc gaggcgccgc cgctggccaa gttcccggcg
     2041 gccacgttcg gcatcctcga gctcaacgcc gaccccgtgc tgccgctgac ggtgcgcaag
     2101 atcgagggcc agctcggcgt gagggccgtg acgctgggcg gcgcgccggc gcgcgacctc
     2161 acgttgaccg acgacgcggc catcatcgac tggtacgcca ggctggcgcg ctacaacgag
     2221 acctcgctgg ctcgatcgac cgtcgagagc gagctgggcg tccggctgcc ggtgccgccg
     2281 aagaaggcgc ccgcgcgcca ggccaaggac catggccgca cgaagtcgca ggaggagcag
     2341 gacgaggacg ccgacgagtc cgacgtcaac gcgcaggatc cgaacgcggt gcagacccgc
     2401 accgtgagcc tgctggccaa ggcgccgggc gtgcacctgg tcaagctgcc gacggagtcg
     2461 cagaccgagc cgcatccgtt cgaggtgatc ggcatcccgc tgccgcagcc gggctaccac
     2521 gtcgtcgaga tcgagtcgcc gcgcctgggc gaggcgctgc tcgatcgcaa ggcgccgatg
     2581 tacgtgcgca ccggcgtgct cgtcaccaac ctggccgtgc acttcaagtg ggcgcccgtg
     2641 aactcgggcg cctgggtcac cacgctggac aagggccagc cggtgcaggg cgcgacggtg
     2701 caggtgtccg actgccgcgg cgagaaggtc tggagcggcc ggaccgacgc gaccggcttc
     2761 gcgcgcatcg accagccgct gccgcgcatg cgctgggagt actgctcgca tgcgggcaac
     2821 gagcgctggg ccggccagac gggccgcccc gagggctact tcgtgagcgc gcgcaagacc
     2881 gacgcgcagg gccgccacga catggccttc gtgtggtcga cctggcagga aggcatcgag
     2941 gcctggcgct tcaacctgaa ctcgcagggc cgcggcgaag aggtctcgca cccgctgttc
     3001 cacacggtga tggaccgcac gctgctgcgc gccggccaga cgatctcgat gaagagccat
     3061 gcgcgccgcg agctgctgac cggcctcgcg ctggtggacg cgggcgacct gccgaccacg
     3121 ctgcgcatcg aacacgaggg cagcgacgac cactacgagt tcccgctcac ctggcgcgcc
     3181 gggcgctatg ccgagacggc gtggaagatt ccggaggacg ccaagctggg cgtgtacgac
     3241 gtgacgctga tccgcgaagg ccgcatctcg gtgtcgaccg gttcgttccg cgtcgaggag
     3301 ttccggctgc ccgcgatggt cggccggctg gtgccgccgc cgggcccgca ggtcgatccg
     3361 cgcgagctca cgatggacgt gatggtgaac tacggcaacg gcggcggtgc ggccggcttg
     3421 ccgctgaagg tgtcggcgca ggagcgcgac atcgacctgt ccagggccat tcccatgcag
     3481 cgctggcccg gcttccactt cgagccgccc aaggccccga gcaccgacca gggcgcgcgc
     3541 gaccccgacg gcgcgatgtt cagcgaggag tacgtcgacg aggacgacgc cgaccgcatg
     3601 gtgacgcaac gcgatccgaa ctcgaagctg gtggccgaca agctgcctgt cacgctcgac
     3661 aagtcgggcg ctggcaaggt gacgctgccc aagctgccgc ccgtcacggc gcccagggaa
     3721 ctgctggtgc aggccaccta cgccgacccg aacggcgagg tgcagacgct gagccagacg
     3781 ctgccgctgt ggccctcggg cgtggtgctg ggggtgcgca ccgacgactg ggtctcggtg
     3841 caccagaagc tgtcgactca ggtcgtcgcg ctcgacacca agggcaggcc gcaggcgggc
     3901 gtgtcggtga gcgtgcgcgc ggtggtgcat cgccagatct cggcgcgcaa gcgcctggtc
     3961 ggcgggttct acgcgtacga caacaggaac gtggacgagg acctgggcga ggtgtgctcc
     4021 ggcagcagcg acgcgcgcgg cctggtgctg tgcgaggccg agctgtcgac ctccggccag
     4081 gtggagctgg tcgcgcaggc caaggacgcg cagggccacc tggcgcgcgc ggcctccagc
     4141 gtgtgggtga cgcgcgcggg cgaggtgtgg ttcggcagcg ccaacgacga ccgcatggac
     4201 gtgctgcccg agcagcgcgc ctacgagccg ggccaggtcg cgaagttcca ggtgcgcagc
     4261 ccgttccgcc acgcgaccgc gctggtggcc atcgagcgca acggcatcgt cgagacccgc
     4321 gaggtccagc tggacggccg cgatccgacc atcgagctgc cggtcaaggc cgagtgggcg
     4381 cccaacgtgt tcgtgtcggt gctcgccgtg cgcggccgcg tgcgcgaggt gccgtggtac
     4441 tcgctgttct cgtggggctg gaagtcgccc gtcgagtggt ggcacgcatg gcgcaacgag
     4501 ggccaggact ggcaggcgcc cacggcgatg gtcgatctgg ccaagccggc gttcaggttc
     4561 ggcatcgcgg agatcgaggt cggagacgcg gcgcaccggc tcaaggtcga cgtgacgccg
     4621 gacaaggcca cgtacccgat ccgcgccacc agccaggtgc gcatcaaggt gacgctgccc
     4681 gacggacgtc ccgcgcccgc gggcaccgag gtgacgctgg cggcggtcga cgaggcgctg
     4741 ctctcgctga aagccaacga cagctgggac ctgttcgacg cgatgatccg ccgccgcgac
     4801 tacggcgtgg agacggccac cgcgcagatg cagatcatcg gcaagcgcca tttcggcaag
     4861 aaggcggcgc ctccgggtgg cggcggcggc cagttcccca cgcgcgagct gttcgacacg
     4921 ctgctgctgt ggaacccgag cgtcgtgctc gatgccaagg gcgaggccgt cgtcggcgtg
     4981 ccgctcaacg actcgctcac gagctttcgc atcgtcgcgg tggccgacgt ggtgcagggc
     5041 gcgcagtcgg cgctgttcgg caccgggcag gcgacgatcg ccgccacgca ggacctgcag
     5101 atcgtctcgg gcgtgccgcc gctggtgcgc gagggcgacc actacaaggc gatgttcacg
     5161 ctgcgcaaca cgtccaaggc ggcgatgaac gcgtcgctgg cggcgtcggc cggcgcgcag
     5221 tcgctgccgg cgcagaccgt ggcgatcgcg gccggggcgg cggcggacgt gtcgtgggac
     5281 gtggacgtgc cgttcaacgt caagagcctt gcctggacgg tggccgccga tgccggcgcg
     5341 gcgcacgacc gcatgaagtt cgcgcagaag gtggccgagg cggtgccggt gacggtgcag
     5401 caggcgaccc tgatgcagct ggacaagacg ctgcgcgtgc ccatcgcgcc gccggtcaac
     5461 gcattgccgg acggcacggg caagctgcgc ggcggcatcg aggtggcgct caagcccagg
     5521 ctgggcgacg gcctgcccgg cgtgcgcgag tacttccagt ggtatccgtg ggactgcttc
     5581 gagcagcgcg cgtcggtggc catcggcctg cgcgacacca cgcgctggca cgcgctggtc
     5641 ggccagatcg cgctgtacca ggacgaggac gggctgctga actactttcc caacgggcga
     5701 ccgcacgacg gcagcgacag cctgaccgcc tacgtgctgg ccatcagcga cgaggccacg
     5761 cgcctgggct acgacttcgc gctgcccgac gacgccaagg ccagcctgca gcgcggcctg
     5821 atcggcttcg tcgagggcca cgtgaaccgc gacttctggc gcccggcctt cctcaagaa
//
LOCUS       DATCRO010000190         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_767329, whole genome shotgun sequence.
ACCESSION   DATCRO010000190 DATCRO010000000
VERSION     DATCRO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767329"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VIN75_05070"
     CDS             <1..870
                     /locus_tag="VIN75_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8973568.1"
                     /translation="VYIATATREHAAHAIAALEAGKAVLCEKPFAIGVEQGQRVLEAA
                     QRSGRLFMEAQWTTALPAFRELLRLARDRALGEPVLLHSEFGIAHDPAAYPRLFEGPG
                     AGVLLDFGVYPIVLALQALGPVRSVDAAVRCNAAGVDVHATLQLTHEHGGHSQLAASL
                     VCALPNRTSLSFAGGHVSLASPVMGAESLQVCPVAPVGAAHAGGPRAGLVAALRRRRW
                     VRRLKAFLGGRAALERPFGVDRYAPQLSHFLALLDAGATESDLMPHATSLAVLRVVEA
                     ARQCRPPVVEASP"
     gene            867..2057
                     /locus_tag="VIN75_05075"
     CDS             867..2057
                     /locus_tag="VIN75_05075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8973569.1"
                     /translation="MKIAYLLNSYPMTSTTFIRREIEALVADGVEVERFAARPWGQKL
                     VDPLDLAEQQRTRYLLAGNVPNLFAAFLRELVANPVRLARAAGVAFRLWRNAREGFGR
                     HVAYLLQGVYFKQQAALSGAVHVHTHFATNATSIALLSHVLGGPGYSFTAHGPDEFEN
                     TDRLSFDLKMTHARFIAAISAFGRVQLIRASRWAHWPKIEIVHCGLALPDFTPVPNPG
                     NQVLVNIARFNSQKGLLLIPAAVAAVKDEFPGLKVVLIGDGEERPALEAEIARRGVGG
                     HIELRGWQSNVEVRRALVESRALLLPSFAEGLPVVIMEAFALGRPVISTFIAGIPELV
                     DASCGWLAPAGDVDALAAAMRDALSADAGRLERMGGEARRRAVARHDIAESARRLKAL
                     FAGG"
     gene            2111..>3221
                     /gene="gmd"
                     /locus_tag="VIN75_05080"
     CDS             2111..>3221
                     /gene="gmd"
                     /locus_tag="VIN75_05080"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY8973570.1"
                     /translation="MTDKKVALITGVTGQDGAYLAEFLLKKGYEVHGIKRRASSFNTD
                     RIDHLYQDPHVNNRDFVLHYGDLTDSTNLTRIVQKVQPDEIYNLAAQSHVAVSFEEPE
                     YTANADGIGALRLLEAIRILGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYA
                     VAKLYAYWITVNYREAYGMYACNGVLFNHESPLRGETFVTRKITRAMARIALGLQDAL
                     YLGNMSALRDWGHARDYVQMQWLMLQQQQAEDFVIATGVQYSVRDFVRMSAERLGITV
                     RFEGQGEAEVGIVDAVTGDKARCKPGQVIVRVDPRYYRPTEVETLLGDPSKAKAKLGW
                     TPTTTLPELVAEMIDADYTTARRDAMVSMAGFQTFS"
BASE COUNT          468 a         1158 c         1128 g          467 t
ORIGIN      
        1 gtctacatcg ccaccgccac gcgcgagcac gccgcccatg cgatcgcggc gctcgaggcc
       61 ggcaaggccg tcctgtgcga gaagccgttc gcgatcggcg tggagcaggg ccagcgcgtg
      121 ctggaggccg cgcagcgcag cggccgcctg ttcatggagg cgcagtggac gaccgcgctg
      181 ccggcgttcc gcgagctgct gcgcctggcg cgcgatcgcg cgctcggcga gccggtgctg
      241 ctgcactcgg agttcggcat cgcgcacgac ccggcggcgt acccgcggct gttcgagggg
      301 cccggcgcgg gcgtgctgct cgatttcggc gtctacccga tcgtgctggc gctgcaggcg
      361 ctcggtccgg tgcgctcggt ggatgccgcc gtgcgctgca acgcggcggg cgtcgacgtg
      421 cacgcgacgc tgcagctcac gcacgagcac ggcggccact cgcagctggc cgcgtcgctg
      481 gtctgcgcct tgccgaaccg cacgagcctg tcgttcgccg gcggccatgt gagcctggcc
      541 tccccggtga tgggcgcgga gagcctgcag gtctgcccgg tcgcgccggt cggcgccgcg
      601 cacgccggcg gcccgcgggc cgggctggtc gccgcgctgc gccgccggcg ctgggtgcgc
      661 cgcctcaagg ccttcctggg tggccgcgcc gccctcgagc gcccgttcgg cgtcgatcgc
      721 tacgcgccgc agctgtcgca cttcctcgcg ctgctcgatg ccggcgcgac cgagagcgac
      781 ctgatgccgc acgccacgtc gctggccgtg ctgcgcgtcg tcgaagcggc ccgacaatgc
      841 cggccccccg tcgtggaagc gtccccgtga agatcgcgta cctcctgaat tcctatccga
      901 tgaccagcac gacgttcatc cgtcgcgaga tcgaggcgct ggtcgccgac ggcgtcgagg
      961 tcgagcgctt cgcggcgcgg ccctggggcc agaagctggt cgacccgctg gacctcgccg
     1021 agcagcagcg cacgcgctac ctgctggccg gcaacgtgcc caacctgttc gcggccttcc
     1081 tgcgcgaact ggtggccaat ccggtgcggc tggcgcgcgc ggcgggcgtc gcgttccggc
     1141 tctggcgcaa cgcgcgcgag ggcttcggcc gccacgtcgc ctacctgctg cagggcgtgt
     1201 acttcaagca gcaggctgcg ctcagcgggg ccgtgcacgt gcacacgcac ttcgccacca
     1261 acgccacgtc gatcgcgctg ctgagccacg tcctcggcgg gccgggctac agcttcaccg
     1321 cgcacgggcc cgacgagttc gagaacaccg accggctcag cttcgacctg aagatgaccc
     1381 acgcgcgctt catcgcggcg atctcggcct tcggccgcgt gcagctgatc cgcgccagcc
     1441 gctgggcgca ctggcccaag atcgagatcg tgcactgcgg cctcgcactg cccgacttca
     1501 cgcccgtgcc caacccgggc aaccaggtgc tggtcaacat cgcgcgcttc aactcgcaga
     1561 agggcctgct gctgatcccg gccgcggtgg ccgcggtcaa ggacgagttc cccggcctga
     1621 aggtggtgct gatcggcgac ggcgaggagc ggccggcgct cgaggccgag atcgcccgcc
     1681 ggggcgtggg cgggcacatc gagctgcgcg ggtggcagtc caacgtggag gtgcggcgcg
     1741 cgttggtgga atcgcgcgcg ctgctgctgc ccagcttcgc ggagggcctg ccggtggtca
     1801 tcatggaggc gttcgcgctc ggccgcccgg tgatctcgac gttcatcgcc ggcatcccgg
     1861 aactggtgga tgcgagctgc ggctggctgg cgcccgcggg cgacgtcgac gcgctcgccg
     1921 ccgcgatgcg cgacgccctg tcggccgatg ccggccgcct ggaacggatg ggcggcgagg
     1981 cccgccgccg cgcggtcgcc aggcatgaca tcgccgagtc ggcacgccgg ctgaaagcgc
     2041 tttttgccgg gggctgatcc ccgtcctttt cagctgatgg aaggccgtcg cccggcgcat
     2101 aatggcccgc atgaccgaca aaaaagtagc gctcatcacc ggtgtcacgg gccaggacgg
     2161 cgcctatctg gccgagttcc tgctgaagaa gggctacgag gtccacggca tcaagcgccg
     2221 ggcatcgtcc ttcaacaccg accgcatcga ccacctctac caggatccgc acgtcaacaa
     2281 ccgcgacttc gtcctgcact acggtgacct gacggactcg acgaacctga cgcgcatcgt
     2341 gcagaaggtg cagcccgacg agatctacaa cctcgcggcg cagagccacg tggccgtcag
     2401 cttcgaggaa cccgagtaca cggccaacgc cgacggcatc ggcgcgctgc gcctgctcga
     2461 ggcgatccgc atcctgggtc tcgagaagaa gacgcgcttc taccaggcct ccacgtcgga
     2521 gctgtacggc ctggtgcagg agatcccgca gaaggagacg acgccgttct acccgcgcag
     2581 cccgtacgcg gtggccaagc tctacgccta ctggatcact gtcaactacc gcgaggccta
     2641 cggcatgtac gcctgcaacg gcgtgctgtt caaccacgag agcccgctgc gcggcgagac
     2701 cttcgtcacg cgcaagatca cccgcgcgat ggcgcgcatc gcgctcggcc tgcaggacgc
     2761 gctgtacctg ggcaacatga gcgcgctgcg ggattggggc catgcgcgcg actacgtgca
     2821 gatgcagtgg ctgatgctgc agcagcagca ggccgaggat ttcgtcatcg ccaccggcgt
     2881 gcagtacagc gtgcgcgact tcgtgcgcat gtccgccgag cgcctgggca tcaccgtgcg
     2941 cttcgagggc cagggcgagg ccgaggtcgg catcgtcgac gccgtcaccg gcgacaaggc
     3001 caggtgcaag ccgggccagg tcatcgtgcg ggtcgatccg cgctactacc ggcccaccga
     3061 ggtcgagacg ctgctgggcg acccgagcaa ggccaaggcc aagctgggct ggacgcccac
     3121 cacgacgctg cccgagctgg tggccgagat gatcgacgcc gactacacca ccgcgcgccg
     3181 cgacgcgatg gtgagcatgg cgggcttcca gaccttcagc c
//
LOCUS       DATCRO010000191         5524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_677087, whole genome shotgun sequence.
ACCESSION   DATCRO010000191 DATCRO010000000
VERSION     DATCRO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677087"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIN75_05085"
     CDS             <1..466
                     /locus_tag="VIN75_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEY8973571.1"
                     /translation="ALGGTRRLGRRAVVLTPHREQVPAPLPADVLWLPYVPLAALLPR
                     AAALVHHGGIGTTAEALRAGVPQVIVPLAYDQFDNAARVAALQAGARESGGTAGARPR
                     ALARTLQRLLDSAEVRAGCARVAALAAADAGRDLGAQVERALDERSVTAGSR"
     gene            672..2732
                     /locus_tag="VIN75_05090"
     CDS             672..2732
                     /locus_tag="VIN75_05090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973572.1"
                     /translation="MAAVLAGLQLLAGDARAIVPSDSMPGEPPSALPVAASFKPVDEF
                     ERLQFVGRAQPELATVGLLRYVDALPVDNPRYLEALFELGSEYVGQNKGDEAEQVAVR
                     IEALSDRLPLARPCAMLLRGQWLQAHGEVSKAERQIIEAQVLLPATLPDWLRLRLLMN
                     SAFVKNRGGHYDEAMARYNQALKLADETGPMWRRIDLRALVASVLFDAGQTDKAAEIN
                     REQMRLATESSDEFGISSAYTMRAIEFSRGRDSGTVLADWRAALEHARLGGSKHQILI
                     GMGNIADYYLQHGDFQTAYELADKALPLAVAANDLPAQSVAVANKGLALIGMHRKDEG
                     LPLVRQSNSIDERSGTAVDISESQHELGGYLERAGYLDDALAAYHQYRQLSEELNQQD
                     RQRALIELQESFANENRQHELDMLAREGRLKDEEIRHHELQVRQWTAAGIVSVLLLAV
                     VAVLARRLRVRNQLLSVSNEQLRLQAVMAAEHADGLEGTLYLLDVDHFKQINDRCGHA
                     GGDTVLVEIARRLRQVLRDDDLIVRWGGEEFLVLVRPLPQGEAEALAQRLLGALADAP
                     VMHDGDPVPVSASIGFGPFPLRRAPMQRELPLNWERAISLVDAAMYLAKAHGRNGACS
                     LRRVDAEGLAQVEEFIEHLEKAAADGRVELHFQQGPSLRQGAPAPTRAPEQPRTPAHG
                     AEVA"
     gene            2732..4759
                     /locus_tag="VIN75_05095"
     CDS             2732..4759
                     /locus_tag="VIN75_05095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973573.1"
                     /translation="MRARSLVLALAAGLALASGAAQAAPDDIQQIDDLVRQGDETPDS
                     AIRAIDEMSNNDFAVSPLLRRASLTGLGIVQARNGRIAEALAQVAALTELGRTEPLAD
                     ADAHLVRAEIEVNGGHMSTSYQQARAALAIYSESCAPGPRQDAACNYRDWWRALDMAQ
                     RGAADQGNAVIANGYAQAMLDVARRNGDRPREAISLGVAALQAEANADSTTANRLIAQ
                     AQHVARADGSAWVQVRVAVLGGLLHFKRQQPEAVQRDYEAALGVANAAGLKRPAMLLR
                     ANLADAYIHQGRPGDALAAIDLALPVAKLHHDSRTERLLLHNRALAKLALGRVAEGKA
                     EMAHVLELWQKDTGPGQRQEALREFGDALGKAGDTAGALELFHREEALTREIRAANKS
                     AAEAELRARYDQDSQKRRIELLGRDNQLKQAELDNQALARRLWMFAGAALVLLGVLAL
                     MMYLRMRELNRTLVKHEALLRAHSERDALTGLANRRQFREVMRVRGGERAFRGALLMV
                     DVDHFKRVNDVHGHAAGDRVLVEVARRLGAAMRADDLVARWGGEEFLVFAPNVEGAEL
                     DQLAERVRVAMNHVPVVLDNGTAIATSVSIGYAAFPLPASHVPVSWEQAVNLADLSLY
                     TAKNHGRNCAVGIVRTTAVSSEALREVEADIAQARDDGRVTLRVSPEAAAA"
     gene            4833..>5524
                     /locus_tag="VIN75_05100"
     CDS             4833..>5524
                     /locus_tag="VIN75_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973574.1"
                     /translation="MTNADLPSSCDVLVVGAGPAGAACALWLARADADVVLVDQHDFP
                     RDKVCGDGLIPDAHHALQRLGVYEEVMAAAQASQHVACIGPRGGRIDVPGRVAVLPRR
                     VLDDIVCRAAVRAGATLRTPWRFEAALSEADAVVGARLKQGETLREVRAKWVVLATGA
                     VPQALQAADMCKRRTPSGIALRGYVRNDAMVGRIKALEVVWHKRLSPGYGWIFPAPGG
                     RFNIGVGIAHSHP"
BASE COUNT          768 a         2068 c         2012 g          676 t
ORIGIN      
        1 cgccctgggt ggcacgcggc gcctgggccg gcgcgccgtc gtcctcaccc cgcaccgcga
       61 gcaggtgccc gcgccgctgc ccgccgacgt gctgtggctg ccctatgtcc cgctggccgc
      121 cctgctgccg cgcgcggccg cgctggtgca ccacggcggc atcggcacca ccgcggaggc
      181 gctgcgcgcc ggcgtgccgc aggtgatcgt gccgctcgcc tacgaccagt tcgacaacgc
      241 ggcgcgcgtg gcggcgctgc aggccggggc gcgcgaatcc ggcggcaccg ccggcgcccg
      301 ccctcgcgcc ctggctcgca cgctgcagcg gctgctcgac agcgccgagg tgcgcgccgg
      361 ctgcgcccgg gtcgcggcgc tggcggcggc cgacgccggg cgcgacctcg gcgcgcaagt
      421 ggaacgcgct ctggacgagc gatccgtcac ggccgggtcg cgctagcgcg acgccccggg
      481 cgcacggggc ggcccgcaac gcgaattgtt tggggaaacc gaggcttcgg cggcgatcag
      541 ggcggcttca ttccgcccag aatccaccga taacgattcc gatgccccag gaaaacctca
      601 ggcgcggcgg ccccagccgc cgtccgccat cggccgcccc cgtcgcgctc cggcgcgcgt
      661 cgggcatcgc catggccgcc gtcctggcgg ggctgcagct gctggccggg gacgcccgcg
      721 ccatcgtccc gtccgattcg atgcccggcg agccgccgag cgcgctgccg gtggccgcgt
      781 cgttcaagcc ggtcgacgag ttcgagcggc tccagttcgt cggccgcgcc cagccggagc
      841 tggccacggt cgggctgctg cgctacgtcg atgcgctgcc ggtcgacaac ccgcgctacc
      901 tcgaggcgct gttcgagctc gggtcggaat acgtcggcca gaacaagggc gacgaggccg
      961 agcaggtggc cgtgcgcatc gaggcgctgt cggaccgcct gccgctggcg cgcccttgcg
     1021 cgatgctgct gcgcggccag tggttgcagg cgcacggcga ggtcagcaag gccgagcgcc
     1081 agatcatcga ggcccaggta ctgctgccgg ccaccctgcc cgactggctg cggctgcgcc
     1141 tgctgatgaa ctcggccttc gtgaagaacc gcggcggcca ctacgacgag gcgatggcgc
     1201 gctacaacca ggcgctcaag ctggccgacg agacgggccc gatgtggcgc cgcatcgacc
     1261 tgcgcgcgct ggtcgccagc gtcctgttcg acgccggcca gaccgacaag gccgccgaga
     1321 tcaaccgcga gcagatgcgc ctggccaccg agtcgtccga cgaattcggc atctcctcgg
     1381 cctacacgat gcgcgccatc gagttcagcc gcggccgcga ttcgggcacc gtgctggccg
     1441 actggcgcgc cgcgctggag cacgcgcgcc tgggcggcag caagcaccag atcctgatcg
     1501 gcatgggcaa catcgccgac tactacctgc agcacggcga cttccagacg gcctacgagc
     1561 tggccgacaa ggcgctgccg ctggccgtgg ccgccaacga ccttcccgcg cagagcgtgg
     1621 cggtcgccaa caagggcctg gcgctgatcg gcatgcatcg caaggacgaa ggcctgccgc
     1681 tcgtgcgcca gtccaacagc atcgacgagc gttcgggcac cgcggtcgac atctccgaat
     1741 cgcagcacga gctcggcggc tacctcgagc gcgccggcta cctcgacgac gcgctggccg
     1801 cctaccacca gtaccgccag ctgtccgagg agctcaacca gcaggaccgc cagcgagcgc
     1861 tgatcgagct gcaggagagc ttcgccaacg agaaccgcca gcacgaactc gacatgctgg
     1921 cccgcgaggg ccgcctgaag gacgaggaga tccgccacca cgagctgcag gtccggcaat
     1981 ggacggccgc cggcatcgtc agcgtgctgc tgctggcggt ggtggccgtg ctggcgcgcc
     2041 ggctgcgggt gcgcaaccag ctgctgtcgg tcagcaacga gcagctgcgc ctgcaggccg
     2101 tgatggccgc cgagcatgcc gacggcctgg aaggcacgct gtacctgctg gacgtcgacc
     2161 acttcaagca gatcaacgat cgctgcggcc acgccggcgg cgacaccgtg ctcgtggaga
     2221 tcgcgcgccg cctgcgccag gtactgcgcg acgacgacct gatcgtgcgc tggggcggcg
     2281 aggagttcct cgtgctcgtg cgcccgctgc cccagggcga ggccgaggcg ctcgcgcagc
     2341 gcctgctggg cgcgctggcc gacgcgccgg tgatgcacga cggcgacccg gtgccggtgt
     2401 ccgcgtcgat cggcttcggc ccgttcccgc tgcggcgcgc gccgatgcag cgcgaactgc
     2461 cgttgaactg ggagcgtgcg atctcgctgg tcgacgccgc gatgtacctg gccaaggcgc
     2521 acggccgcaa cggcgcctgc agcctgcgcc gcgtcgacgc cgagggcctc gcgcaggtcg
     2581 aggagttcat cgagcacctc gagaaggcgg ccgccgacgg ccgcgtcgag ctgcatttcc
     2641 agcagggccc gagcctgcgc cagggcgcgc ccgcccccac gcgcgcgccc gagcagccgc
     2701 gcacgccggc gcacggcgcg gaggtggcgt gatgcgcgcg cgcagcctcg tcctcgccct
     2761 ggccgccggc ctcgcgctgg ccagcggcgc cgcgcaggcc gcgccggacg acatccagca
     2821 gatcgacgac ctggtgcgcc agggcgacga gacgcccgac agcgcgatcc gcgccatcga
     2881 cgagatgtcg aacaacgatt tcgccgtctc gccgctgctg cggcgcgcat ccctcaccgg
     2941 cctgggcatc gtgcaggcgc gcaacgggcg catcgccgag gcgctcgcgc aggtcgccgc
     3001 gctgaccgag ctcggccgca ccgagccgct ggccgacgcc gacgcccacc tggtgcgagc
     3061 ggagatcgag gtcaacggcg ggcacatgtc gacgtcgtac cagcaggccc gcgcggcgct
     3121 ggcgatctac agcgagtcgt gcgcgccggg cccgcgccag gacgccgcgt gcaactaccg
     3181 cgactggtgg cgcgcgctgg acatggccca gcgcggcgcc gccgaccagg gcaacgccgt
     3241 catcgccaac ggctacgcgc aggccatgct ggacgtcgcg cgccgcaacg gcgaccgccc
     3301 gcgcgaggcg atctcgctgg gcgtggccgc gctgcaggcg gaggccaatg ccgactccac
     3361 cacggccaac cggctcatcg cgcaggcgca gcacgtggca cgcgccgacg gctcggcctg
     3421 ggtgcaggtg cgcgtcgcgg tgctgggcgg cctgctgcac ttcaagcgcc agcagcccga
     3481 ggccgtgcag cgcgactacg aggccgcgct cggcgtggcc aacgcagccg gcctgaagcg
     3541 cccggccatg ctgctgcgcg ccaacctggc cgacgcctac atccaccagg gccgccccgg
     3601 cgatgcgctg gcggccatcg acctcgcgct gccggtggcc aagctgcacc acgactcccg
     3661 caccgaacgg ctgctgctgc acaaccgcgc gctggccaag ctggcgctgg gccgcgtggc
     3721 cgaaggcaag gccgagatgg cgcacgtgct ggagctgtgg cagaaggaca cgggccccgg
     3781 ccagcgccag gaggccctgc gcgagttcgg cgacgccctg ggcaaggcag gcgacaccgc
     3841 gggcgcgctc gagctgttcc atcgcgagga agcgctgacc cgggagatcc gcgcggccaa
     3901 caagtccgcg gccgaggccg aactgcgcgc ccgctacgac caggattcgc agaagcgccg
     3961 catcgagctg ctgggccgcg acaaccagct caagcaggcc gagctcgaca accaggccct
     4021 cgcgcgccgc ctgtggatgt tcgccggcgc cgcgctggtg ctgctgggcg tgctggcgct
     4081 gatgatgtac ctgcgcatgc gggagctgaa tcgcacgctc gtcaagcacg aggcgctgct
     4141 gcgcgcgcac agcgagcgcg acgccctcac gggcctggcc aaccggcgcc agttccgcga
     4201 ggtgatgcgc gtgcgcggcg gcgagcgcgc gttccgcggg gcgctgctga tggtggacgt
     4261 cgaccacttc aagcgcgtca acgatgtcca cggccacgcc gcgggcgacc gcgtgctggt
     4321 ggaggtggcg cgccggctgg gcgccgcgat gcgcgccgac gacctggtgg cccgctgggg
     4381 cggcgaggag ttcctcgtgt tcgcgcccaa cgtcgagggc gcggagctcg accagctcgc
     4441 cgagcgcgtg cgcgtcgcga tgaaccatgt gccggtcgtg ctggacaacg gcacggccat
     4501 cgcgacctcg gtgtcgatcg gctacgccgc cttcccgctg cccgccagcc atgtgcccgt
     4561 ctcctgggag caggcggtca acctggccga cctgtcgctc tacacggcca agaaccacgg
     4621 gcgcaactgc gcggtgggca tcgtgcgcac gacggcggtc tccagcgagg cgctgcgcga
     4681 agtcgaggcc gacatcgccc aggcccgcga cgacggacgc gtcacgctgc gcgtgagccc
     4741 ggaggccgcg gcggcttgac cccggcgcag ggatcctgcg accgcgcgca ggatgacgac
     4801 gtcgcgagcc tgccggggat aatcccccgc tcatgacgaa tgccgacctt ccctcttcct
     4861 gcgacgtcct cgtcgtcggc gccggcccgg ccggggccgc gtgcgccctg tggctggcgc
     4921 gcgccgacgc cgatgtcgtg ctcgtcgacc agcacgactt cccgcgcgac aaggtctgcg
     4981 gggacggcct gatcccggac gcccaccacg cgctgcagcg gctgggcgtg tacgaggagg
     5041 tgatggcggc ggcgcaggcc tcgcagcacg tggcctgcat cggcccgcgc ggcgggcgca
     5101 tcgacgtgcc cggccgcgtg gccgtgctgc cacgccgcgt cctcgacgac atcgtctgcc
     5161 gcgccgccgt gcgcgccggc gcgacgctgc gcacgccgtg gcgcttcgag gcggcgctct
     5221 ccgaggccga cgccgtggtc ggcgcgcgcc tcaagcaggg cgagacgctg cgcgaggtgc
     5281 gcgcgaagtg ggtggtgctg gccaccggcg ccgtgcccca ggccttgcag gccgccgaca
     5341 tgtgcaagcg ccgcacgccc agcggcatcg cgctgcgcgg ctacgtgagg aacgacgcga
     5401 tggtgggccg catcaaggcg ctcgaggtcg tctggcacaa gcggctcagc cccggctacg
     5461 gctggatctt cccggctccg ggcggtcgct tcaacatcgg cgtgggcatc gcgcacagcc
     5521 atcc
//
LOCUS       DATCRO010000192         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_47211, whole genome shotgun sequence.
ACCESSION   DATCRO010000192 DATCRO010000000
VERSION     DATCRO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47211"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1157)
                     /locus_tag="VIN75_05105"
     CDS             complement(213..1157)
                     /locus_tag="VIN75_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973575.1"
                     /translation="MAKQILSSSRLALAAGLLAVSFSGPGAAAGFGADPTAGKRTPDA
                     APASQWRSWSYQTLDYPGAAQTIFWGLDDFGGLAGQYTIAGGVAHAMTYRHGNFQPLD
                     PDKLGTYFSAAGGPNDAGTTFGGYADASGTQHGFVSRRGRFETVDFPGHLNSNVDAVD
                     GDGTILGVYWDADHVFHGMLSRNGNETPIDVAGARDTYPLGLNSKGDSVGYWDAAGSA
                     THGYVRRADGQVASLDIPDAAATVAFAINDRGQVAGYFTDRSGALHGFVKTGEQVQQL
                     DMPGAVATIVTALNNCDSVAGEYRDAGGVRHGFMALLK"
     gene            1292..1834
                     /locus_tag="VIN75_05110"
     CDS             1292..1834
                     /locus_tag="VIN75_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY8973576.1"
                     /translation="MTADLSITEILARASAGDAAAIDGLIPRIYATLHRLAHRQRLGE
                     QAHTLDTTALVHEAWLQLAGRRDVSFPDRAHLYAYMSRAMRHILVDHARRHRAQKRQA
                     PLVLEEPAGPDILDVLAIDQAMERLAAVDERLARVAELRLFADLSSVEISHVLGVTAR
                     TVERDWLKARTFLGACLSPD"
     gene            1904..>3565
                     /locus_tag="VIN75_05115"
     CDS             1904..>3565
                     /locus_tag="VIN75_05115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8973577.1"
                     /translation="MIDISARWLEIEPLIDTLFDVPAAERAEWLRTHCADPDVRDLVA
                     RALDDAPGVQSLERGIAQWMPSLVEGLDDVLPVVDGYRVLRFVGAGGMASVFEALRQL
                     PGGPQTVALKLLRLDVHDADERRGFLREQRILARLQHPHIAQLLDAGFSPSGTPFLAL
                     EFVAGDNLVAHCDRLSLGTRERLVLFMDVCAAVEHAHRNLIVHRDLKPSNVLVDGEGR
                     VKLVDFGIAKLLGGDAEPTRTQARRMTRAYAAPEQFAGDVTTTSIDVYALGVLLAELL
                     GGQRARRGADACEADAPVFDDEALRRALGADLHAIVRQATRRDPLRRYPDVAVMRDDV
                     QRHLAGRPLLARADGVLVRVGKFARRHAFGVAAGLLAATLLAGSTAIGLHEASLARAA
                     AAQTHAQALAAEDEARRARALKSFLEGLFDNATQGAAANDTTALLLAQGRERVERDFA
                     LQPALHVEILALIGDLERRSGHPGRAQQPLEEAAALARAQFGVTDRRTLHIEYLLAKE
                     GDELGHVRDAVARLQGAVDAFEAGPERGSPDEAPALAWLAGLYERH"
BASE COUNT          472 a         1265 c         1323 g          505 t
ORIGIN      
        1 gggtgatggg cggcgcgatc cccgccgtgc cgcggggacg tcgccgtcgt ggccgatccg
       61 tcgagcgcgc agattagaag gcgccatcct tcagccagct ttcaacgcga cggggcgggg
      121 gaaaatactt agcggggggc cgggctccgt cggcgggccg cggccgaccc gcgggtcggg
      181 gccggcctcg attcaacggc gcgccggccg catcacttca gcaaggccat gaagccgtgg
      241 cgcacgccgc ccgcgtcgcg gtattcgccg gcgacgctgt cgcagttgtt gagggcggtg
      301 acgatcgtcg cgacggcgcc gggcatgtcc agctgctgga cctgttcccc ggtcttgacg
      361 aagccgtgga gcgcgcccga cctgtccgtg aagtagccgg cgacctggcc gcggtcgttg
      421 atcgcgaagg ccacggtggc cgccgcgtcg gggatgtcca gcgacgcgac ctggccgtcc
      481 gcgcgccgca cgtagccgtg ggtcgccgag ccggccgcgt cccagtagcc gacggaatcg
      541 cccttgctgt tcaggcccag cgggtaggtg tcgcgcgcgc cggccacgtc gatcggcgtc
      601 tcgttgccgt tgcggctcag catgccgtgg aagacgtggt ccgcatccca gtagacgccc
      661 aggatcgtgc cgtcgccatc gacggcgtcg acgttcgagt tcaggtggcc ggggaagtcg
      721 acggtctcga agcggccgcg ccggctcacg aagccgtgct gcgtgcccga cgcgtcggcg
      781 tagccgccga aggtcgtgcc ggcgtcgttg gggccgccgg cggcgctgaa gtacgtgccc
      841 agcttgtcgg ggtccagcgg ctggaagttc ccgtggcggt aggtcatggc gtgggccacg
      901 ccgcccgcga tcgtgtactg gccggcgagc ccgccgaagt cgtccaggcc ccagaagatc
      961 gtctgcgcgg cgccggggta gtcgagcgtc tggtacgacc acgaccgcca ctggctggcc
     1021 ggcgcggcgt ctggtgtacg cttgcccgcg gtcggatcgg ctccgaatcc ggcggccgcg
     1081 ccgggcccgg agaacgagac cgcgagaagg ccggcggcca gggcgagtcg gctggacgaa
     1141 aggatctgct ttgccatgaa ggcgctccat gaagtggctg agtggacgat ggcgctccgt
     1201 gcaccatctc ctccatgagc gcagagcgcc tgcccgcacc gacattgccc tggaaaaaaa
     1261 tccgcgacgc cgccgcgtcg cccgctggcg catgactgcc gacctgtcca tcactgaaat
     1321 cctcgcgcgc gcgtccgcgg gcgacgccgc cgccatcgac ggcctgatcc cgcggatcta
     1381 cgcgacgctg catcgactcg cgcaccgcca gcgcctgggc gagcaggccc acacgctgga
     1441 cacgaccgcg ctcgtgcacg aggcctggct gcagctggcc gggcgcaggg acgtgtcctt
     1501 tcccgatcgc gcccacctgt acgcctacat gagccgggcg atgcgccaca tcctggtcga
     1561 ccacgcgcgg cgccatcgcg cgcagaagcg ccaggcgccg ctggtgctgg aggagccggc
     1621 cgggcccgac atcctggacg tgctcgcgat cgaccaggcg atggagcggc tcgcggcggt
     1681 cgacgaacgc ctcgcgcggg tcgcggagct gcgcctgttc gccgacctgt cgagcgtgga
     1741 gatctcgcac gtcctgggcg tgaccgcgcg caccgtggag cgcgactggc tcaaggcgcg
     1801 cacgttcctg ggcgcctgcc tgtcgccgga ctgaagcgga tgtcggtgtt cgcgacgcga
     1861 ttgcgctgat gggtcgggag gatgcgacga tgcgtcgctg accatgatcg acatctccgc
     1921 gcgctggctc gagatcgagc ccctgatcga cacgctgttc gacgtgcccg ccgccgagcg
     1981 cgccgagtgg ctgcgcacgc attgcgccga cccggacgtg cgcgacctgg tcgcgcgggc
     2041 gctcgacgat gctcccggcg tgcagtcgct cgaacgcggc atcgcgcagt ggatgccgtc
     2101 gctcgtcgag ggcctggacg acgtgctgcc ggtcgtggac ggctatcgcg tgctgcgttt
     2161 cgtgggcgcg ggcggcatgg ccagcgtgtt cgaggcgctg cggcaattgc ccggcggccc
     2221 gcagacggtc gcgctgaagc tgctgcgact ggacgtgcac gacgccgacg agcgccgcgg
     2281 cttcctgcgc gagcagcgca tcctggcgcg cctgcaacat ccgcacatcg cccagctgct
     2341 cgatgcgggc ttctcgccga gcggcacgcc attcctcgcg ctggagttcg tggccggcga
     2401 caacctcgtc gcgcactgtg accgcctctc gctcggcacg cgcgagcggc tcgtcctgtt
     2461 catggacgtg tgcgcggcgg tggagcacgc gcatcgcaac ctgatcgtgc atcgcgacct
     2521 caagcccagc aacgtgctgg tggacgggga gggacgcgtc aagctcgtgg acttcggcat
     2581 cgccaagctg ctgggcggcg acgccgagcc gacgcgcacg caggcccggc gcatgacgcg
     2641 ggcctatgcg gcgcccgaac agttcgcggg cgacgtgacg acgacctcga tcgacgtcta
     2701 tgccctgggc gtcctcctgg ccgaactgct gggtggacag cgtgcacgcc gcggcgccga
     2761 cgcgtgcgag gccgacgccc cggtcttcga cgacgaggcc ttgcgccgtg cgctcggagc
     2821 ggacctgcac gcgatcgtgc gccaggccac gcgtcgcgat ccgctgcggc gctatccgga
     2881 cgtggccgtg atgcgggacg acgtccagcg ccatctcgcc ggccggcccc tgctggcgcg
     2941 cgcggacggc gtcctcgtgc gcgtgggcaa gttcgccagg cgtcatgcgt tcggcgtcgc
     3001 cgcgggcctg ctggcggcca cgctgctggc cggcagcacg gcgatcggcc tgcacgaggc
     3061 gagcctcgcg cgcgccgcgg ccgcgcagac gcacgctcag gcgctcgcgg ccgaggacga
     3121 ggcgcggcgt gcccgggcgc tcaagtcctt cctcgagggc ctgttcgaca acgcgacgca
     3181 gggcgcggcg gccaacgaca ccaccgcgct gctgctcgcg cagggccgcg agcgggtcga
     3241 gcgcgacttc gcgctgcaac cggcgctgca tgtcgagatc ctcgcgctga tcggcgacct
     3301 cgagcgccgc agcggccatc ccggtcgcgc gcagcagccg ctcgaggagg cggccgccct
     3361 ggcgcgcgcg cagttcggcg tcaccgaccg gcgcacgctg cacatcgaat acctgctcgc
     3421 caaggaaggc gacgagctcg gccatgtgcg cgacgccgtc gcgcgactgc agggcgcggt
     3481 cgacgcgttc gaggcggggc ccgagcgcgg ctcgccggat gaggcgccgg cgctggcctg
     3541 gctggcgggc ctgtacgaac ggcac
//
LOCUS       DATCRO010000193         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_418584, whole genome shotgun sequence.
ACCESSION   DATCRO010000193 DATCRO010000000
VERSION     DATCRO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418584"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..883)
                     /locus_tag="VIN75_05120"
     CDS             complement(140..883)
                     /locus_tag="VIN75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8973578.1"
                     /translation="MNDDFSVDAADQTLRGTVTGNTAGDAPSVLALHGGGQSSRRGIQ
                     YLLSALARDGVASASFDFSGHGDSTGQVEGSSLNERMAQALAVAGHVAPGRPLSLIGT
                     SMGGHVACSLIERLQPPALVLFCPAAYVAPAQDAPFGPSFQQILRSTVDYASSPAFTA
                     LEGFEGRLLLVHGTQDAVIPAQVLDQYERRARRAESVEVVRLEGAGHKLHDWLQSRPR
                     DHGLVLDAVLRTLAPGARRQSAPGGISFT"
     gene            complement(915..1850)
                     /locus_tag="VIN75_05125"
     CDS             complement(915..1850)
                     /locus_tag="VIN75_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8973579.1"
                     /translation="MDSKTKRPAGIGLAEVMLLLVAAVWGGSYAVAKQATQQLPVLEF
                     IALRFGLTFLVLLPALKPLFGVGWQRGLAVGGLLGANLLAIFVCETFGVSLTTASNAA
                     FLISLCVALTPFVEWWLLGQRPAPRMFRAAGLSALGAAMLSATSPAELSVGWGDGLMV
                     IAAFLRAVMVCMTRRLAGHHAMPALTLTAVRSGVMALGAGALALVAAPAGGAWHVPPA
                     TASFWWGMAYLVLLCTVFAFFAQNHAASRSSPSRVSLLMGSEPVFGALIAAYGFGERI
                     GPWGWAGGLLIVCAAWWVTMPARETPGRVVASDAA"
BASE COUNT          333 a          739 c          705 g          257 t
ORIGIN      
        1 gcgacggcct gcgtggcttg cgatcgctgc ggatccgcgg gtgccgccgc gtcggtttcg
       61 gcgtgcgccg agacggcggc gaccgccagc gcgatgccgc atgcgcgcca ggcggcgcgc
      121 gctccctgtt tcttcgaaat catgtgaacg aaattcctcc cggcgcggat tgtctccgag
      181 ccccgggggc cagcgtccgc agcacggcat cgagcacgag cccgtggtcc cgtggacggg
      241 actggagcca gtcgtgcagc ttgtgccccg cgccttcgag ccgcacgacc tcgacggact
      301 cggccctgcg ggccctgcgc tcgtactggt cgaggacctg cgcgggaatc acggcgtcct
      361 gcgttccgtg gaccagcagc aggcggccct cgaagccttc gagcgccgtg aaggccggcg
      421 agctcgcgta gtccacggtc gagcgcagga tctgctggaa gctcgggccg aagggcgcgt
      481 cctgggccgg ggccacatag gctgccgggc agaagagcac cagcgcgggc ggctgcagcc
      541 gctcgatcag ggaacacgcg acgtggccgc ccatgctcgt gccgatcagc gagagcggcc
      601 gccccggcgc cacgtggccg gccacggcca gcgcctgcgc catgcgttcg ttcaggctgg
      661 agccttcgac ctggccggtg ctgtctccgt gaccggagaa gtcgaaggag gcgctcgcga
      721 cgccatcgcg cgccagagcc gagagaaggt actggatgcc gcgccgcgag gactggccgc
      781 cgccgtgcag agccagcacg ctcggcgcgt cgccagcggt gttgccggtg accgtgccgc
      841 gcagcgtttg atcggccgca tcaacggaga agtcgtcgtt catcgcgcga gcatgacacg
      901 gcccgcccgc cctctcaggc ggcatcgctg gcgacaacgc ggcccggagt ctccctcgcc
      961 ggcatcgtca cccaccacgc cgcgcagacg atcagcaagc cccctgccca gccccacggc
     1021 ccgatgcgct cgccgaagcc gtaggccgcg atcagcgcgc cgaacaccgg ctcgctgccc
     1081 atcagcagcg acacgcggct cgggctcgaa cgcgaggccg catggttctg cgcgaagaag
     1141 gcgaagacgg tgcacagcag cacgaggtag gccatgcccc accagaagga ggccgtggca
     1201 ggcggaacgt gccatgcgcc gccggccggc gcggcgacga gcgcgagcgc ccccgcgccg
     1261 agggccatca cgcccgaccg caccgccgtc agcgtcagcg ccggcatcgc atggtgcccg
     1321 gcgagccgtc gcgtcatgca gaccatcacg gcgcgcagga aggccgcgat caccatcagg
     1381 ccatcgcccc agcccaccga gagttccgcg ggcgaagtgg ccgagagcat cgccgcgccc
     1441 agcgccgaca ggccggcagc ccggaacatg cgcggcgccg gccgctgccc cagcagccac
     1501 cattcgacga agggcgtgag cgcgacgcac aggctgatga ggaaggcggc attgctggcc
     1561 gtggtgagcg acacgccgaa ggtctcgcag acgaagatcg ccagcaggtt cgcaccgagc
     1621 aggccgccga cggcgaggcc cctctgccac ccgacgccga acagcggctt cagcgcgggc
     1681 agcagcacga ggaaggtcag cccgaagcgc agcgcgatga actccagcac cggcagctgc
     1741 tgcgtcgcct gcttggccac tgcatagctg ccgccccaca ccgccgccac cagcagcagc
     1801 atgacttcgg cgaggccgat gcccgccggc ctcttcgtct ttgaatccat acttcgcttg
     1861 cgccgtccgt gaacatttcc gagaacccga cgcatccgtc gggcatggaa ttgttccggg
     1921 catcacccgg gccgcacagg cgtcggccgg aacaggacta ttgcgtcgtg ggaacgaatt
     1981 catttctcaa ggtgctgccg gagatggtga ccttcgtgcg ggtcgccgag ctgg
//
LOCUS       DATCRO010000194         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_688710, whole genome shotgun sequence.
ACCESSION   DATCRO010000194 DATCRO010000000
VERSION     DATCRO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688710"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1574)
                     /gene="xseA"
                     /locus_tag="VIN75_05130"
     CDS             complement(213..1574)
                     /gene="xseA"
                     /locus_tag="VIN75_05130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8973580.1"
                     /translation="MPAPDAPTPPSRHILTPSTLNRLVRDLLEDALPLVWIEGELSNV
                     ARPASGHLYFTLKDANAQVRCAMFRPKAIGLRFRPADGAHVLVRARVGLYEPRGEFQL
                     VAEHMEPAGEGALQREFEQLKARLDAEGLFDRSRKRPLPAFARRIGVITSATGAAVRD
                     VLSVLSRRWPLAEVDVLPVPVQGREAPPAIVSMLRRASASGRYDALLLTRGGGSLEDL
                     WSFNDERLARAIAASPVPVVSAVGHETDFSLSDFAADLRAPTPSVAAELLVPSRDDLL
                     RRLRALDARLRNLQAQQLRAAMQRADRAALRLNALRPRARLDALCKRQADALRRLSAA
                     WQRRIDRERARVRHADAVLRAAHPQRRIARLRERLAAVSSRPQAAVVRRLGNETLRLR
                     GLARSLEAVSPLATVARGYAILRHPDGRVVRSVLDAAPGDRLDARLADGQLPLRVIDP
                     GEA"
     gene            complement(1698..>2350)
                     /locus_tag="VIN75_05135"
     CDS             complement(1698..>2350)
                     /locus_tag="VIN75_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8973581.1"
                     /translation="LLVPGSSDIPVVAGMSRSGYRPLLKAGIRVFEWNGSMLHAKTAV
                     ADGLWARVGSSNLNIASWLSNREIDVAVEDAAFAQQLAAQYEQDLANATEIVLTPRPN
                     RPYKDRVSASARRTRTGRMGGSSSRAAAGALRIANTMGAALSDRRVLGDTETGPLLAG
                     TAALAALAAVAILWPAVVGWPIAVLAAWFALNFGIRAWRARRRHQRRIEAADEPDA"
BASE COUNT          330 a          802 c          895 g          323 t
ORIGIN      
        1 cgcgcttgcc ggtgtacttg tcgaggacgt ccagttcctg cttcccgtcg accggctggt
       61 tggcgaggta atagggatag cgcctggcga gcatcggaat cctccttgtt tcgcgaagcc
      121 tagatcatgc cgtgtaaacg cggcatgggt ggtgttgcag gcgaccttgc gaaggtgtcg
      181 cgcggcgtcg gctcgcgatc tgcgggcgac cgtcacgctt cgccgggatc gatcacccgc
      241 agcggcagct ggccgtcggc cagtcgcgcg tccaggcgat cgccgggcgc ggcgtctagc
      301 acgctgcgca cgacgcgtcc gtcgggatga cgcaggatcg cgtagccgcg tgcgacggtg
      361 gcgagcgggc tgaccgcttc gagcgagcgc gcgagcccgc gcagccgcag cgtctcgttg
      421 cccagccgcc gcaccaccgc ggcctgcggg cgcgaagaca cggcggccag gcgttcgcgc
      481 aggcgcgcga tgcggcgctg tgggtgcgcc gcgcgcagta ccgcgtcggc atggcggacg
      541 cgggcgcgtt cgcgatcgat gcgccgctgc caggcggccg acaggcgccg cagcgcgtcc
      601 gcctgacgct tgcacaaggc atcgagccgc gcgcgcggac gcaatgcgtt caggcgcagt
      661 gcggcgcggt cggcgcgctg catcgccgcg cgcaattgct gtgcctgcag gttgcgcagg
      721 cgtgcatcca gtgcgcgcaa ccggcggagg agatcgtcgc ggctgggcac cagcagttcc
      781 gccgcgaccg agggcgtcgg cgcgcgcagg tcggcggcga aatcggacag gctgaagtcg
      841 gtttcgtggc cgactgccga caccacgggc acgggcgagg cggcgatcgc gcgcgccagg
      901 cgctcgtcgt tgaaagacca caggtcctcg agcgatccgc cgccgcgcgt gagcagcaac
      961 gcgtcgtagc ggccgctggc ggaggcgcgg cgcagcatcg agacgatcgc cggcggcgct
     1021 tcgcggccct gcaccggcac cggcagtacg tccacttcgg ccagtggcca gcggcgcgac
     1081 agcacgctca gcacgtcgcg cacggcggcg ccggtggcgg aggtgatcac gccgatgcgg
     1141 cgggcgaagg cgggcagggg gcgcttgcgc gagcggtcga acaggccttc ggcgtcgagc
     1201 ctggccttga gctgttcgaa ctcgcgctgc agggcgcctt cgccggccgg ctccatatgc
     1261 tcggccacca gctggaattc gccgcgcggt tcgtacaggc cgacgcgggc gcgcaccagc
     1321 acgtgggcgc cgtcggcggg gcggaagcgc aggccgatgg ccttggggcg gaacatggcg
     1381 caacgcacct gggcgttggc atctttcagg gtgaagtaca ggtgcccgga ggccggccgc
     1441 gccacgttcg acagctcgcc ctcgatccac accagcggca gcgcatcctc gagcaggtcg
     1501 cgcaccagcc ggttgagcgt gctcggcgta aggatgtgcc gcgagggcgg cgtgggagcg
     1561 tcgggggcag gcatggaaag cgggagacta gcgcaggaac tgccgcgctg tcatgagctt
     1621 gccgcaaagt gttgaagtgt aggttggggt gagccgcagg cgaaccccaa cggtgcggca
     1681 acgttcggtt cgccggctca ggcgtccggc tcgtccgccg cctcgatccg ccgctggtgc
     1741 ctgcgccgcg cccgccaggc gcggatgcca aagttcagcg cgaaccatgc cgccagcacc
     1801 gcgatcggcc agcccaccac ggccggccac aggatcgcca ccgccgccag cgccgccagc
     1861 gcggcggtgc cggccagcag cgggccggtc tcggtgtcgc ccagtacgcg gcggtcgctg
     1921 agcgcggcgc ccatggtgtt ggcgatgcgc agggcgccgg cggcggcgcg gctggaactg
     1981 ccgcccatgc ggccggtgcg ggtacgccgg gcggaggcgc tgacgcggtc cttgtacggg
     2041 cggttcggac gcggggtcag cacgatctcg gtggcattgg ccaggtcctg ctcgtactgc
     2101 gcggccagct gctgggcgaa ggcggcgtct tccaccgcca cgtcgatctc gcggttgctc
     2161 agccagctgg cgatattgag attggacgaa cccacccgcg cccagagccc gtcggctacc
     2221 gccgtcttgg cgtgcagcat cgagccgttc cattcgaaca cgcggatgcc cgctttgagc
     2281 agcggccggt agccggaacg cgacatgccg gcgaccaccg ggatatcgct gctgcccgga
     2341 accagcaggc
//
LOCUS       DATCRO010000195        15884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_362028, whole genome shotgun sequence.
ACCESSION   DATCRO010000195 DATCRO010000000
VERSION     DATCRO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362028"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="adh"
                     /locus_tag="VIN75_05140"
     CDS             complement(<1..1031)
                     /gene="adh"
                     /locus_tag="VIN75_05140"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8973582.1"
                     /translation="MDMLEPGRFGYAVNFKKRYGNYIGGEWVPPVKGEYFENITPVTG
                     RTFCEIPRSSADDVELALDAAHAAKAAWGRTSPAERANILNRIADRMEQNLELIATAE
                     TWDNGKPIRETLAADIPLAIDHFRYFAGCVRGQEGGISEIDHTTYAYHFHEPLGVVGQ
                     IIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASILVLMELIGDLLPKGVVNVVNG
                     FGLECGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFEDVM
                     RQDDAFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYDRFIERAVQRVAAIKQGNPL
                     DKSTMIGAQA"
     gene            complement(1275..2417)
                     /locus_tag="VIN75_05145"
     CDS             complement(1275..2417)
                     /locus_tag="VIN75_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8973583.1"
                     /translation="MKTSRHAAILGAAIAAAAGHAGAADFKAGDWDLSVGGIVNAFYT
                     RTHCGGSQAVGGLALATQALGCGGDDGRTVIGNGLLPNALIISAKGSQDGIDIGATLM
                     IGAAASSDDAIGNNSNVDVRQGFLTFGTRDAGSVKLGRDYGLFGRSAILGDMTLFGAG
                     MPVAATQRNRVTLGHIGAGYTYLGTYGQVAYAAPSMGGFAATVALMSPVDASGTAFVG
                     RTSPQIQAMASFAFTGGKVWAGLKSQKFDGPAGTGFTMNGAEIGGSATLGDFGLLANV
                     QSGKGIGVLSDGDSGPRRQTNVAVQGTFQATPKLKLGLNWGESRLRDGVVGTDLRSNA
                     NLTGGLYFALTRSLTLTGELSRTMSKDMAGDTARLDGVAAGAILFF"
     gene            complement(2690..4966)
                     /locus_tag="VIN75_05150"
     CDS             complement(2690..4966)
                     /locus_tag="VIN75_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973584.1"
                     /translation="MRGAHRLRLLAVPLALARALAWAEEPTQVASLQSVVVVGTTVLP
                     GVGTPLSQVTANVQTLRGDALASAHAATLAQALDQGVGSVNVNDTAGNPYQLDVNFRG
                     FTASPALGTPQGLSVFVDGVRVNEVFGDTVNWDLIPAGAIDRLSVIPGSNPVFGLNTL
                     GGALSVTTRDVFGEDGGQVRATGGAWGRQGLEAEAGGHGARAGWFVTGNALREQGWGT
                     HDPSRVAQAFGKLGWRDGADHADVSLSLASTRLEGNQTLPLSWLADFRQAYSWPDRQI
                     NHLAFLNANANHRFSPTLLLEANAFHRALRSFVRNSNVNDDFDTAEAIGIGNEPTANA
                     INDIRQARTGASLQLTSAAAIAGHANHLVAGASVEGGFTRFTQSDQEAGASRDTTSTR
                     PLTLRTSLHARAADAGLYATDTFGFEAHDFLVVSGRYDVAKVVLADQLGTALDGNHSF
                     RRFNPAIGLTSNPTPGLTLYAAYNEGMRVPTPVELTCADPAAPCALPNAFSSDPALKP
                     VISRTTELGARGKSGEALEWSIALFHTDLRDDIQFISSGGGATSAGYFRNVGATRRQG
                     VELGLRSQLDALTLAARYSVVDATFRTPLVLNSPNNSTAAPISCAGCTDIAVRPGDRL
                     PGIARRTLKVDARWTFRKGAVVGVTMVSQGPTHARGDENNADANGRVPGFTLFNLDAR
                     MPLGPGWEAFANVDNVFGRRTSTFGTLGRNVFTGPGRTFDVTGASWRSEQFRVAGAPR
                     GIWAGMTWRFGAQAADAS"
     gene            complement(4971..5294)
                     /locus_tag="VIN75_05155"
     CDS             complement(4971..5294)
                     /locus_tag="VIN75_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY8973585.1"
                     /translation="MTDTTRRIFIARSLAGGCAVAGLGLATSARAAAHVEETDESAVG
                     LGYHHDTRLTDQKKYPAHTPQQRCVDCAFWQATASDAWGGCAMFGRKQVAAGGWCGAF
                     AKKPG"
     gene            complement(5370..7187)
                     /locus_tag="VIN75_05160"
     CDS             complement(5370..7187)
                     /locus_tag="VIN75_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HEY8973586.1"
                     /translation="MQSRLLTLMIAGGLATAFGAAGAAGGVTDQMIQSEATATGNVLT
                     WGVNTQGQRYSPLRQVNTATVSRLAPVWAFSFGGEKQRGQESQPVIYNGRMFVTGSYS
                     RIFALDAATGRKLWKYEHRLPEGIMPCCDVVNRGAALFDNLVIFATLDAQLVALDQAT
                     GDVVWKTKIDDYAAGYSASAAPLIAQGLLLTGVSGGEFGVVGRVEARDPRTGKLVWTR
                     PTVEGHMGYTYDPDGTKHENGVSGTPGQTWPGDLWKTGGAATWLGGTYDAKTGLAYFG
                     AGNPAPWNSHMRKGDNLFSCSTIAIDVKTGQIKWHYQATPNDGWDFDGVNEFVTFDMD
                     GRRMGGKADRNGFFYVIDANDGKLMNAFPFVRKITWASSIDLKTGRPNFIESSRPGDP
                     TQGQDGQKGASIFNAPSFLGGKNQMPMAYSPDTHLFYVPSNEWGMEIWNEPVAYKKGA
                     AYLGAGFTIKTINDDYIGALRAVDPKTGKIVWEVKDTAPLWGGVLTTGGNLVFWGTPE
                     GYLKAADAKTGKVLWQFQTGSGVVAPPVTWQQGGEQYVSVVSGWGGAVPLWGGEVAKK
                     VNMLEQGGMVWVFKIPRAGGALAPKQAAAEPATKVAAAH"
     gene            complement(7393..7782)
                     /locus_tag="VIN75_05165"
     CDS             complement(7393..7782)
                     /locus_tag="VIN75_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973587.1"
                     /translation="MIVGERDVQLPVWPLFRQDGTAVPLVGRVYESVDLAPIPGFSGT
                     PAKLLVALDARGAFMDVQVLSQHEPAHGVRHALVDAREYAMAGHDPAGARWTLDIADP
                     IAEGRLVARPSADGRWGATPGPALATA"
     gene            complement(7885..8859)
                     /locus_tag="VIN75_05170"
     CDS             complement(7885..8859)
                     /locus_tag="VIN75_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY8973588.1"
                     /translation="MTAPLRSLFAAALLLASGAARAGTGLVYIANEKDDTLTLVDART
                     LAVAGSIPTCARPRHLQRLPGGTRLLVACGDAGQADVIDLATRRVVDSSPLGHDPEAL
                     DLSADGRLAYVSAEDDQALAIFDLAAKKEAGRVALGPEPEGVKLSPDGRRVYVTSEVA
                     GVIYAIDTATLKIEKTIPVGKRPRRLALTADGAQLWVTNEKSATISVISTATLEVVDT
                     VPIAVEGLAADGITPVGMALAPDGATMYVALGRANRVAFVDVAARKVRALVPVGRRAW
                     ALALSADGGRLFVAGGLSNDLTAIDTRDARVLKTIAVGRVPDAVLVDD"
     gene            complement(8856..10037)
                     /gene="pqqE"
                     /locus_tag="VIN75_05175"
     CDS             complement(8856..10037)
                     /gene="pqqE"
                     /locus_tag="VIN75_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY8973589.1"
                     /translation="MPPFWLLAELTYRCPLHCVFCSNPLDYARHDAELDTAAWKRILG
                     EARAMGAVQLGFSGGEPLLRDDLEELVAHARSLGFYTNLITSGVGLTAARARALKDAG
                     LDHVQLSFQDSTRELNDFISSTRTFDMKSRVAAIIKDAGYPMVLNCVMHRFNLPHVGR
                     IVDMAERMGADFLELANTQYYGWAWHNRAALMPTVDELRDAEAIVVAHRERLASVGGA
                     MKILWVSPDYADAKPKPCMAGWGAVFLVVAPDGVALPCHSARMLPGFEFPNLRDTAVR
                     DAWFDSAAFNAYRGDAWMNDTCGTCDERHADHGGCRCQAFLLTGDAAATDPVCRRSEH
                     RERVDALLDAAATAALERTREAPLRFVPGAATGGGLVFRGDAASLALSAPANELQAAT
                     P"
     gene            complement(10052..10330)
                     /gene="pqqD"
                     /locus_tag="VIN75_05180"
     CDS             complement(10052..10330)
                     /gene="pqqD"
                     /locus_tag="VIN75_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY8973590.1"
                     /translation="MIDSHARPAVAPMYRLQFEPAQDAWVLLYPEGMVKLNTPAAEIL
                     RRCDGQRSVEAIVDDLERAFACDGLTGDVHAFLSQARERGWVDPGAPS"
     gene            complement(10327..11070)
                     /gene="pqqC"
                     /locus_tag="VIN75_05185"
     CDS             complement(10327..11070)
                     /gene="pqqC"
                     /locus_tag="VIN75_05185"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY8973591.1"
                     /translation="MNDRFPLDTPWTAEDFEARLRERESRYHIHHPFNLRLNRGELAP
                     FQVRGWVANRFYYQSQIPRKDAAVLANCDDRAERRRWIERILDHDGRGDFRDGQAGGI
                     EAWCQLATATGLSRKDLESMRQVQPGVRFAVDAYVNFARSAPWQEAAISSLTELFAPK
                     IHADRLAGWPSLYPWIAAEGLAYFRSRIPLASRDVTHGLEVARRWCTTRERQERAIAI
                     LDFKLDILWSMLDAIALAYPDDLAKELRP"
     gene            complement(11067..11999)
                     /gene="pqqB"
                     /locus_tag="VIN75_05190"
     CDS             complement(11067..11999)
                     /gene="pqqB"
                     /locus_tag="VIN75_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY8973592.1"
                     /translation="MKILVLGSGAGGGFPQWNCNCANCTGQRSGRIRASERTQSSIAV
                     SADGESWVLLNASPDIGSQIRRHPQLHPRHGLRDTPIKAVVLMDAQVDHVTGLLGLRE
                     GPCIDLWATRCVFEDLTSGLPLLAVLRHYCGTRWHMIGVDGDSARHEFEIEAAPGLRF
                     TALAIPGMAPPYSPHRREQVTGDNIALRVEDVADGSSLFYSPGLARVGEQERAWMDAA
                     DCVLVDGTFWQEDEMQAAGLGTKSASEMGHLPQQGAAGRPGMVDALRDASPARKVLIH
                     INNSNPILDEDSAPRRALAAMGIEVAHDGMEISL"
     gene            complement(12066..12140)
                     /gene="pqqA"
                     /locus_tag="VIN75_05195"
     CDS             complement(12066..12140)
                     /gene="pqqA"
                     /locus_tag="VIN75_05195"
                     /inference="COORDINATES: protein motif:HMM:NF001400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEY8973593.1"
                     /translation="MQWTQPAFEDLRFGFEITMYIAAR"
     gene            complement(12485..14572)
                     /locus_tag="VIN75_05200"
     CDS             complement(12485..14572)
                     /locus_tag="VIN75_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY8973594.1"
                     /translation="MSASLRALPIPGGWTTPTAADATARAELIEQSHLRCTALGLTRI
                     ERPDFEPMIRGDLVVARERNQRLLTHAAPVMEMLFEQIVNTESMIVLTDAQGTILHSI
                     GDDEFLARAGKVALAPGVNWAEQSKGTNAIGTALVEERPTLVHAGEHFIHANSFLTCS
                     AAPIVDPRGNMLGVLDVTGDQVSYHQHTMGLVRMSARMIENHWLSEDYGNRLRLHFHL
                     RPEFIGTLLEGIIVVGGDGRILGANRSALDQLDVSGAALRMHTITSLFETTIGAVIDH
                     FRAPIPLPMRLTMSNGRQFHASARFNGPLRSVMAGLEGAPATRADHDAAPAAATTASV
                     PHRAAADESARRACLSGLHYLSTGDAQVEAVVTKVQRVLNKEIPLLILGETGTGKELL
                     ARAVHQDSNRARQPFVAVNCASIPESLIEAELFGYEDGAFTGARRKGATGRILQANGG
                     TLFLDEIGDMPLSLQARLLRVLQERCVTPLGSHKSIPVDIAVVSATHRNLREMCAAST
                     FREDLYYRLNGLVVRLPALRDRSDLRTVARRIVLAESPEGPPEIGASVMALFERYAWP
                     GNIRQLANVLRTAVVMAGSEPQITEEHLSEDFLEDVRRQPAATPAPVSVAASATASMP
                     PPASRTAVVPEAAATFAAPAPTSGAMPARTLGESEIALIQAALDAAGGNISEASKALG
                     ISRNTIYRKLRWR"
     gene            14930..15460
                     /gene="pedF"
                     /locus_tag="VIN75_05205"
     CDS             14930..15460
                     /gene="pedF"
                     /locus_tag="VIN75_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HEY8973595.1"
                     /translation="MRRHAHPALPPTNQRPRRLAAAVRIGSLLLTLGACVVVAAHGNV
                     TPQPVDTAGLPAVGQDWLSVNPYSGNPKAVEIGSSGFNQNCARCHGLEAISGGIAPDL
                     RRLDNDCAGMKDPAARDGCIKDNDEYMITTVRHGRTRNGAVYMPPFEGVLSQEAMWAI
                     KTYLESRREKPLQDAQ"
     gene            15457..>15884
                     /locus_tag="VIN75_05210"
     CDS             15457..>15884
                     /locus_tag="VIN75_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8973596.1"
                     /translation="MTAARIDRRGALRALGAAALVASGGVRAAGTGLEDVKARGTLTV
                     AVYREMPPFHDKGQGIDVDLAAALAGALGVKLSLMPFPAGEDMNDDLRNMVWKGHYLG
                     YGPADVMLHVPVESPLMDANPQVSIFAPYWRDRVMIARDVS"
BASE COUNT         2175 a         5493 c         5798 g         2418 t
ORIGIN      
        1 gcctgcgcgc cgatcatggt cgacttgtcc agcgggttgc cctgcttgat ggcggccact
       61 cgctgcacgg cgcgctcgat gaagcggtcg tagatcgact cctgcaccag cacgcgcgac
      121 gggcaggtgc agacctcgcc ctggttgagc gcgaacatcg cgaagccttc cagcgccttg
      181 tcgaagaacg cgtcgtcctg gcgcatcacg tcctcgaaga agatgttggg cgacttgccg
      241 cccagttcca gcgtcaccgg gatcaggttc tggctggcgt actgcatgat caggcggccg
      301 gtggtggtct cgccggtgaa cgcgatcttg gcgatgcgct tgttcgacgc cagcggcttg
      361 ccgcactcca ggccgaagcc gttgaccacg ttgacaacgc ccttgggcag caggtcgccg
      421 atcagttcca tcagcacgag gatcgacgcg ggcgtctgct ccgcgggctt gagcacgacg
      481 cagttgccgg ccgccagcgc cggcgccagc ttccacacgg ccatcaggat ggggaagttc
      541 cacgggatga tctggcccac gacgcccagc ggttcgtgga agtggtaggc gtaggtggtg
      601 tggtcgatct cgctgatgcc gccctcctgg ccgcgcacgc agccggcgaa gtagcggaag
      661 tggtcgatgg ccagcgggat gtcggcggcc aacgtctcgc ggatcggctt gccgttgtcc
      721 caggtctcgg cagtcgcgat gagctcgagg ttctgctcca tgcggtcggc gatgcggttg
      781 aggatgttcg cgcgctccgc gggcgaggta cggccccagg cggccttggc ggcgtgcgcg
      841 gcgtcaagcg ccagttcgac gtcgtcggcc gacgagcgcg ggatctcgca gaacgtgcgc
      901 cccgtgacgg gcgtgatgtt ctcgaagtat tcgcccttga ccggcggcac ccattcgccg
      961 ccgatgtagt tgccgtagcg cttcttgaag ttgaccgcgt agccgaagcg gccgggttcg
     1021 agcatgtcca tgaaagtctc ctaggtggtc tgggccccgg tcgaagacag cgtccttggg
     1081 gcgaagtgga actgtcggaa ggcgtactgc gatttgcgtg ccaaggcgcg gagaaggagg
     1141 ccgatgtccc gcccgggccg gtggcctgcg ggtcactacg gacgctcgat cgcggcgccg
     1201 tgggcgcgac atgcagcgcg ccgggccgcg tgtgccagaa cggcacaccc cggcccatcg
     1261 cgacacagtc gctctcagaa gaacaggatg gcgccggccg cgacgccgtc gagcctggcc
     1321 gtgtcgcccg ccatgtcctt cgacatcgtg cgactcagtt cgccggtcag cgtcaggctc
     1381 ctcgtgagcg cgaagtacag gccgccggtg aggttcgcgt tgctgcgcag atcggtgccg
     1441 accacgccgt cgcgcaggcg gctctcgccc cagttcaggc ccagcttcag cttgggcgtc
     1501 gcctggaacg tgccctgcac ggcgacgttg gtctgcctgc gcgggccgga gtcgccgtcc
     1561 gacaggacgc cgatgccctt gcccgactgc acgttggcca gcagcccgaa atcgcccagc
     1621 gtggcgctgc cgccgatctc ggcaccgttc atcgtgaagc ccgtgcccgc cggaccgtcg
     1681 aacttctggc tcttgaggcc cgcccagacc ttgccgcccg tgaacgcgaa ggacgccatg
     1741 gcctggatct gcggcgacgt gcggcccacg aatgcggtgc cgctcgcgtc caccgggctc
     1801 atgagcgcca cggtggccgc gaagccgccc atcgacggcg ccgcgtatgc cacctggcca
     1861 tacgtgccca ggtacgtgta gccggcgccg atgtggccca gcgtgacgcg gttgcgctgc
     1921 gtcgctgcca cgggcatgcc ggcgccgaac agcgtcatgt cgccgaggat ggccgaccgg
     1981 ccgaacagcc cgtagtcgcg gcccagcttc acgctgccgg cgtccctggt gccgaacgtg
     2041 aggaagccct ggcgcacgtc gacgttgctg ttgttgccga tcgcgtcgtc cgaggacgcg
     2101 gccgcgccga tcatcagcgt cgcgccgatg tcgatgccgt cctgcgagcc cttggcgctg
     2161 atgatcagcg cattcggcag caggccgttg ccgatcacgg tgcggccgtc gtcgccgccg
     2221 cagccgagcg cctgcgtggc gagcgcgagg ccgccgacgg cctgcgatcc gccgcaatgc
     2281 gtgcgcgtgt agaaggcgtt gacgatgccg ccaaccgaca ggtcccagtc gcccgccttg
     2341 aagtcggccg cgccggcgtg acccgcggcg gcggcgatcg ccgcgccgag gatggcggcg
     2401 tgtcgtgagg tcttcatgtc tcttgtctcc tggtgtgtgg atgtgcgggc ccgggaatgc
     2461 gtgcgacccg tggccgcgtt tcgcaagcga tatgccagtg ccaggaggga gcgttccaac
     2521 ggtcggttgc gtcggcgcgt gacggcggat gtccccgacc gcgacgcgcc gcggcgcgac
     2581 ggccgcgtgg aggaagggat tgctcgcatg tgctgtcccg cacgccgcgt gcgatgtgcc
     2641 aatccgatgg agcgaccgcg cggcgggtgt cgcgcggcag gacagtgcct caggacgcgt
     2701 cggcggcctg cgcgccgaag cgccatgtca tgcccgccca gatgccgcgc ggcgcgcccg
     2761 cgacgcggaa ctgctcgctg cgccagctgg cgcccgtgac gtcgaacgtg cggccggggc
     2821 cggtgaacac gttccgcccc agcgtgccga aggtcgacgt gcgacggccg aacacgttgt
     2881 cgacgttggc gaaggcttcc cagccggggc ccagcggcat gcgcgcgtcg aggttgaaca
     2941 gcgtgaaacc cggcacgcgg ccgttggcgt cggcgttgtt ctcgtcgccg cgcgcatgcg
     3001 tggggccctg cgagaccatc gtcacgccga cgaccgcgcc cttgcggaac gtccaccgcg
     3061 cgtcgacctt gagcgtgcgg cgcgcgatcc cgggcaggcg gtcgccgggg cgcacggcga
     3121 tgtccgtgca gcccgcgcaa ctgatcggcg cggccgtcga attgttcggg ctgttcagga
     3181 ccagcggcgt gcggaaggtt gcgtcgacga cgctgtagcg cgccgccagc gtcagcgcat
     3241 cgagctgtga ccgcagcccc agttccacgc cttggcgtcg tgtggcgccg acgttcctga
     3301 agtagcccgc gctcgtcgcg ccgccgccgc tgctgatgaa ctggatgtcg tcgcgcaggt
     3361 ccgtgtggaa cagcgcgatg ctccattcga gcgcttcgcc cgacttgccg cgcgcgccga
     3421 gctcggtggt gcgcgagatc accggcttga gcgcgggatc cgacgagaac gcgttgggca
     3481 gcgcgcacgg cgcggccggg tcggcgcagg tcagttccac cggcgtggga acgcgcatgc
     3541 cctcgttgta ggcggcgtac aacgtgagcc cgggcgtcgg gttcgacgtg aggccgatcg
     3601 cggggttgaa ccggcggaag ctgtggttgc cgtccagcgc ggtgccgagc tggtcggcga
     3661 ggacgacctt cgcgacgtcg tagcgcccgg agacgacgag gaaatcgtgc gcctcgaacc
     3721 cgaaggtgtc ggtcgcgtac aggccggcat cggcggcgcg cgcatgcagc gaagtccgca
     3781 gcgtgagcgg ccgtgtcgac gtcgtgtcgc gcgacgcgcc ggcctcctgg tccgactgcg
     3841 tgaaccgcgt gaagccgccc tcgacgctcg cgcccgcgac caggtggttc gcgtggccgg
     3901 cgatcgcggc ggccgacgtc agttgcagag acgcgcccgt gcgcgcctgg cggatgtcgt
     3961 tgatcgcgtt ggcggtcggc tcgttgccga tgccgatggc ctcggcggtg tcgaagtcgt
     4021 cgttgacgtt gctgttcctg acgaagctgc gcaatgcgcg gtggaacgcg ttggcctcca
     4081 gcagcagcgt gggcgagaag cgatggttcg cgttcgcgtt caggaacgcc aggtggttga
     4141 tctgccggtc gggccaggaa tacgcctgcc tgaagtccgc cagccagctc aggggcagcg
     4201 tctggttgcc ctcgagccgc gtgcttgcaa gcgacaggct cacgtccgcg tggtctgcgc
     4261 cgtcccgcca gccgagcttg ccgaacgcct gcgcgacgcg gctcgggtcg tgggtgcccc
     4321 agccctgctc gcgcagcgcg ttcccggtga cgaaccagcc ggcgcgcgcg ccgtggccgc
     4381 cggcctcggc ctcgaggccc tggcggcccc aggcgccgcc ggtcgcgcgc acctggccgc
     4441 cgtcctcgcc gaagacgtcg cgggtcgtca ccgacagcgc accgcccagc gtgttgaggc
     4501 cgaacaccgg gttcgagccg gggatcaccg acaggcgatc gatcgcgccg gccggaatca
     4561 ggtcccagtt gaccgtgtcg ccgaacacct cgttgacgcg cacgccatcg acgaacaccg
     4621 acagcccctg cggcgtgccg agcgcgggcg acgcggtgaa gccgcggaag ttgacgtcca
     4681 gctggtacgg gttgcctgcg gtgtcattga cgttgacgct gccgacgccc tggtcgagcg
     4741 cctgcgccag cgtcgcggcg tgcgcagacg cgagcgcatc gccgcgcagc gtctgcacgt
     4801 tggcggtcac ctgcgacaac ggcgtgccga cgccgggcag cacggtggtg ccgacgacga
     4861 ccaccgattg cagggatgcg acctgcgtgg gctcctcggc ccacgcgagc gcccgcgcga
     4921 gcgcgagcgg cacggcgagc aggcgcaggc gatgcgcgcc ccgcatcgcc tcagcccggc
     4981 ttcttcgcga aggcgccgca ccagccgccg gcggccacct gcttgcggcc gaacatcgcg
     5041 cagcctcccc aggcgtcgct ggccgtggcc tgccagaacg cgcagtcgac gcagcgctgc
     5101 tgcggcgtgt gggcggggta cttcttctgg tcggtgagcc gggtgtcgtg gtgatagccg
     5161 aggccgacgg cgctctcgtc cgtctcctcg acatgggcgg ccgcgcgggc cgaggtcgcc
     5221 aggcccaggc ccgcgacggc gcagccaccg gccagcgagc gagcgatgaa gatgcgtcgt
     5281 gtcgtgtcag tcatggaaat ctccgtgcgg gcctgcccgc gcgcgtcacg ctcgcgcgcg
     5341 cacgagccgg cccgcgttct ctcgagcgat caatgggcgg ccgcgacctt ggtggcgggt
     5401 tcggcggcgg cctgcttcgg cgcgagagcg ccgccggccc tggggatctt gaagacccac
     5461 accatgccgc cctgctcgag catgttgacc ttcttggcca cctcgccgcc ccacagcggc
     5521 acggcgccgc cccagccgga gaccacgctc acgtactgct cgccgccctg ctgccaggtc
     5581 accggcgggg cgacgacgcc cgagcccgtc tggaactgcc acagcacctt gcccgtcttc
     5641 gcgtcggcgg ccttcagata cccctccgga gtaccccaga acaccaggtt gccgccggtg
     5701 gtcagcacgc cgccccacag cggcgccgtg tccttgacct cccagacgat cttgcccgtc
     5761 ttcggatcga ccgcgcgcag cgcgccgatg tagtcatcgt tgatcgtctt gatggtgaag
     5821 cccgcgccga ggtaggccgc gcccttcttg taggcgacgg gctcgttcca gatctccatg
     5881 ccccactcgt tggacggcac gtagaacagg tgcgtgtcgg ggctgtaggc catcggcatc
     5941 tggttcttgc cgccgaggaa gctgggcgcg ttgaagatgg acgcgccctt ctggccgtcc
     6001 tggccctgcg tcgggtcgcc ggggcggctg gactcgatga agttcgggcg gcccgtcttc
     6061 aggtcgatgc tgctggccca ggtgatcttg cgcacgaacg ggaaggcgtt catcagcttg
     6121 ccgtcgttcg cgtcgatcac gtagaagaag ccgttgcggt cggccttgcc gcccatgcgc
     6181 ctgccgtcca tgtcgaaggt gacgaactcg ttgacgccgt cgaagtccca gccgtcattg
     6241 ggcgtggcct ggtagtgcca cttgatctgg ccggtcttca cgtcgatggc gatcgtggag
     6301 cacgagaaca ggttgtcgcc cttgcgcatg tggctgttcc acggcgcggg attgccggcg
     6361 ccgaagtagg ccaggccggt cttggcgtcg taggtgccgc ccagccacgt ggcggcgccg
     6421 ccggtcttcc acaggtcgcc gggccaggtc tggccgggcg tgccgctgac gccgttctcg
     6481 tgcttcgtgc cgtcggggtc gtaggtgtag cccatgtggc cttcgacggt cgggcgcgtc
     6541 cacaccagct tgccggtgcg cgggtcgcgc gcctcgacgc ggcccaccac gccgaactcg
     6601 ccgcccgaca cgccggtcag cagcaggccc tgcgcgatca gcggcgccgc cgacgcggag
     6661 tagcccgccg catagtcgtc gatcttggtc ttccacacca cgtcgcccgt ggcctggtcc
     6721 agcgcgacga gctgcgcatc gagcgtggcg aagatgacca ggttgtcgaa cagcgccgct
     6781 ccgcggttga cgacgtcgca gcagggcatg atgccctcgg gcaggcggtg ctcgtacttc
     6841 cacagcttcc tgccggtggc cgcgtcgagc gcgaagatgc gcgagtacga acccgtcacg
     6901 aacatgcggc cgttgtagat caccggctgc gattcctgac cgcgctgctt ctcgccgccg
     6961 aacgagaacg cccagacggg cgcgagccgc gacacggtcg cggtgttgac ctgcctgagc
     7021 ggcgaatagc gctggccctg cgtgttcacg ccccaggtca gcacgttgcc cgtggcggtg
     7081 gcctcggact ggatcatctg gtcggtgacg ccgcccgcgg cgcccgctgc gccgaaggcc
     7141 gtggcgagcc cgccggcgat catcagcgtc aagagtctgg attgcatgtt gtctcctcgt
     7201 tggggttgct gcgacgtccg cgcgccgcgt ggccgtcgcg tggaactgcg caatgtccgt
     7261 gccatgcgcg ttgccgtcga tcgaggcgcg ccgcgcgcgt ccggcgggcg tcgggttgcg
     7321 ggtttccccg ttcgatccgg cgcggactgc tacagcttcg aacacggccg ctcgggcacg
     7381 tgacacggcg actcaggcgg tcgcgagcgc cggtccgggc gtcgcgcccc agcggccgtc
     7441 cgcggacggg cgcgcgacga ggcgcccctc ggcgatcgga tcggcgatgt ccagcgtcca
     7501 gcgcgcgccg gcggggtcgt ggccggccat cgcatactcg cgggcgtcga ccagcgcatg
     7561 ccgcacgccg tgcgccggct cgtgctgcga cagcacctgc acgtccatga acgcgcccct
     7621 ggcgtcgagc gcgacgagca gcttcgcggg cgtgcccgag aagcccggga tcggcgcgag
     7681 gtcgaccgat tcgtagacgc ggcccaccag cggcaccgcc gtgccgtcct gccggaacag
     7741 cggccacacg ggcaactgga cgtcccgctc gccgacgatc aacggcgatg ggaagcgctg
     7801 ctgcaggtcc ccgcttgcgc gacgccgacc atcgcgcaag cgccggcgag cgccagcacg
     7861 acgtaccgcg ccagggcgcg ccagtcagtc gtcgaccagc acggcatcag gcacccgtcc
     7921 caccgcgatc gtcttgagca cccgggcgtc ccgcgtgtcg atggcggtga ggtcgttcga
     7981 caggccgccc gccacgaaca gtcgcccgcc gtcggcagac agcgccagcg cccacgcacg
     8041 ccggcccacc ggcaccagcg cgcggacctt gcgcgcggca acatcgacga acgcgacgcg
     8101 gttggcgcgg ccgagtgcca cgtacatcgt cgcgccgtcc ggtgcgaggg ccatgcccac
     8161 cggcgtgatg ccgtcggccg cgagtccctc gaccgcgatg ggcaccgtgt cgaccacctc
     8221 gagcgtcgcg gtcgagatca cgctgatcgt cgcgctcttc tcgttggtga cccacagctg
     8281 cgcgccgtcg gcggtgagcg cgaggcgccg cgggcgcttg ccgaccggga tggtcttctc
     8341 gatcttcagc gtcgcggtgt cgatcgcgta gatcacgccc gcgacctccg acgtgacgta
     8401 gacgcgccgg ccgtccggcg acagcttgac gccctcgggc tccgggccca gcgcgacgcg
     8461 gccggcttcc ttcttcgcag cgagatcgaa gatcgcgagc gcctggtcgt cctcggcgga
     8521 gacataggcg agccggccgt cggcggacag gtcgagcgcc tcggggtcgt ggcccagcgg
     8581 gctcgagtcg acgacgcggc gcgtggcgag gtcgatcacg tcggcctgcc ccgcgtcgcc
     8641 gcaggccacg agcaggcgcg tgccgccggg aagccgctgc aggtggcgcg gacgcgcgca
     8701 ggtggggatg ctgccggcga ccgcaagcgt cctcgcgtcc acgagcgtca acgtgtcgtc
     8761 cttctcgttg gcgatgtaga cgagcccggt gccggcgcgg gcggcccccg acgcgagcaa
     8821 cagcgcggcg gcgaacaggg agcgcagcgg tgcggtcatg gcgtggcggc ctgcagttcg
     8881 ttggcgggcg cggacagcgc gagcgaggcc gcgtcgccgc ggaagacgag cccgccgccg
     8941 gtggccgcgc cgggcacgaa gcgcagcggc gcctcgcgcg tccgttcgag cgcggccgtg
     9001 gccgcggcgt cgagcagcgc gtcgacgcgt tcgcggtgct cgctcctgcg gcacacgggg
     9061 tcggtggcgg cggcgtcgcc ggtcagcagg aaggcctggc agcggcagcc gccgtggtcg
     9121 gcgtggcgct cgtcgcacgt gccgcaggtg tcgttcatcc aggcgtcgcc gcgataggcg
     9181 ttgaaggcgg cgctgtcgaa ccaggcgtca cgcacggcgg tgtcgcgcag gttcgggaac
     9241 tcgaaacccg gcagcatgcg tgcgctgtgg cacggcaacg cgaccccatc cggggcgacg
     9301 accaggaaca ccgcgcccca gccggccatg cagggcttgg gcttcgcatc ggcgtagtcg
     9361 ggcgacaccc acaggatctt catcgcgccg cccaccgacg ccagccgctc gcggtgcgcg
     9421 acgacgatgg cctcggcgtc gcgcagctcg tcgacggtgg gcatcagcgc ggcgcggttg
     9481 tgccaggccc agccgtagta ctgcgtgttg gcgagctcga ggaagtcggc gcccatgcgc
     9541 tcggccatgt cgacgatgcg gccgacgtgc ggcaggttga agcggtgcat cacgcagttg
     9601 agcaccatcg gatagccggc gtccttgatg atcgcggcca cgcgcgactt catgtcgaag
     9661 gtgcgcgtcg agctgatgaa gtcgttgagc tcgcgcgtcg agtcctggaa cgacagctgc
     9721 acgtggtcga ggccggcgtc cttgagcgcg cgggcccgcg ccgcagtgag gcccacgccc
     9781 gaggtgatga ggttggtgta gaagccgagg ctgcgcgcgt gggcgacgag ctcctcgaga
     9841 tcgtcgcgca gcagcggctc gccgccggaa aaacccagtt gcaccgcgcc catcgcgcgg
     9901 gcttcgccca agatgcgctt ccaggcggcg gtatcgagct cggcgtcgtg gcgcgcatag
     9961 tccagcgggt tggagcagaa cacgcagtgc agcgggcagc ggtacgtgag ttcggccagc
    10021 agccagaacg gcggcatcgc ggcggcgggg ttcacgacgg cgctcccggg tcgacccagc
    10081 cgcgctcgcg cgcctgggac aggaaggcat ggacgtcgcc cgtcagcccg tcgcacgcga
    10141 acgcgcgctc gaggtcgtcg acgatcgcct ccaccgagcg ctggccgtcg cagcggcgca
    10201 ggatctccgc ggccggcgtg ttgagcttga ccatcccctc ggggtacagc agcacccacg
    10261 cgtcctgcgc cggctcgaac tgcaggcggt acatcggggc gaccgcgggg cgcgcatggg
    10321 aatcgatcat ggacgcagtt ccttggcgag gtcgtcggga taggccagcg cgatcgcgtc
    10381 gagcatggac cagaggatgt cgagcttgaa gtcgaggatc gcgatcgcgc gctcctggcg
    10441 ctcgcgcgtg gtgcaccagc ggcgcgcgac ttccaggccg tgcgtcacgt cgcggctggc
    10501 cagcgggatg cggctgcgga agtaggcgag gccctcggcg gcgatccacg gatacagcga
    10561 gggccagccg gcgagacggt cggcgtggat cttcggcgcg aacagctcgg tcagcgacga
    10621 gatcgcggcc tcctgccacg gcgcgctgcg ggcgaagttg acgtaggcgt cgacggcgaa
    10681 gcgcacgccg ggctgcacct ggcgcatcga ttcgaggtcc ttgcgcgaga ggcccgtggc
    10741 ggtggcgagc tggcaccagg cctcgatgcc gccggcctgc ccgtcgcgga aatcgccgcg
    10801 gccgtcgtgg tcgaggatgc gctcgatcca gcggcgccgc tcggcgcggt cgtcgcagtt
    10861 ggccagcacc gcggcgtcct tgcgcgggat ctgcgactgg tagtagaagc ggttggcgac
    10921 ccagccgcgc acctggaacg gcgcgagctc gccgcggttc aggcgcaggt tgaacgggtg
    10981 gtggatgtgg taccgcgatt cgcgttcgcg caggcgcgcc tcgaagtcct cggcggtcca
    11041 cggggtgtcg agagggaagc ggtcgttcac agcgagatct ccatgccgtc gtgcgccacc
    11101 tcgatgccca tcgccgccag cgcgcgccgc ggcgcgctgt cctcgtcgag gatcgggttg
    11161 ctgttgttga tgtggatcag caccttgcgc gccggcgagg cgtcgcgcaa cgcgtcgacc
    11221 atgcccgggc ggccggcggc gccctgttgc ggcaggtggc ccatctcgct ggccgacttc
    11281 gtgcccagcc cggcggcctg catctcgtct tcctgccaga aggtgccgtc gacgagcacg
    11341 cagtcggccg cgtccatcca cgcgcgctcc tgctcgccga cccgtgcgag gccgggcgaa
    11401 tagaacaggc tgctgccgtc ggcgacgtcc tcgacgcgca gcgcgatgtt gtcgccggtc
    11461 acctgctcgc gccggtgcgg cgaatagggc ggcgccatgc cggggatcgc gagcgcggtg
    11521 aagcgcaggc ccggcgcggc ctcgatctcg aactcatggc gggcgctgtc gccgtcgaca
    11581 ccgatcatgt gccaacgcgt gccgcagtag tgccgcagca ccgccagcag cggcaggccg
    11641 ctggtgaggt cctcgaacac gcagcgcgtg gcccacaggt cgatgcaggg cccctcgcgc
    11701 aggcccagca ggccggtgac gtggtcgacc tgcgcgtcca tcagcaccac ggccttgatc
    11761 ggcgtgtcgc gcaggccgtg gcgcgggtgc agttgcgggt ggcggcggat ctggctgccg
    11821 atgtcgggcg aggcgttgag cagcacccag gactcgccgt cggccgagac ggcgatggag
    11881 ctctgcgtgc gctcggatgc gcggatgcgg ccgctgcgct gtcctgtgca gttggcgcag
    11941 ttgcagttcc actgcggaaa gccgccgccg gcgccggagc ccagcacgag gattttcatg
    12001 atcgagtctc gcgttcagag ggcgacgcga acgcgggcgc gatgccgcgt cgcccgtggc
    12061 cggggtcagc gcgcggcgat gtacatcgtg atctcgaagc cgaagcgcag gtcttcaaag
    12121 gcgggttgcg tccattgcat ggggagtctc ctggtgggtt gggacgccgc cggtcggcgg
    12181 tcgccgcgcc cgcattcggc ggacacgagc gccacttcgc aagtcgcgtg ccgcgcgtct
    12241 cgatcccacg ctttccgcgc gatttcgagg gttcaccccg tgccgcggcg cctcctgcgg
    12301 gtgtggccgc gccgttgcgc gcgtcacgcg cgcgggaggt gttccacgct ggaacagggg
    12361 gacgcgttgc ttggaacagg gttcacccgt gtgcctgttt gcccgcttca tggtgtcgag
    12421 cggccgcgcg caccccgccc gtcatccagc tcggggcgat cgcaggagca ggatgaccgc
    12481 tggctcagcg ccagcgcagc ttgcgataga tcgtgttgcg gctgatcccg agcgccttcg
    12541 aggcctcgga aatgttgccg ccggcggcgt cgagcgcggc ctggatcagc gcgatctcgg
    12601 actcgccgag cgtgcgcgcc ggcatcgcgc cggacgtcgg cgcgggggcg gcgaaagtgg
    12661 ctgccgcctc gggaacgacg gccgtccgcg acgcgggcgg cggcatcgac gcggtggctg
    12721 acgccgccac ggacaccggc gcaggcgtcg ccgcgggctg gcggcgcacg tcctcgagga
    12781 aatcctccga caggtgttcc tcggtgatct gcggctcgct gccggccatc acgaccgcgg
    12841 tgcgcagcac gttggcgagc tggcggatgt tgccgggcca ggcatagcgc tcgaacagcg
    12901 ccatcaccga cgcgccgatc tcgggtggtc cctcgggcga ctcggccagc acgatgcggc
    12961 gcgccacggt gcgcaggtcg ctgcgatcgc gcagcgcggg caggcgcacc accaggccgt
    13021 tgagccggta gtagaggtcc tcgcggaagg tcgacgccgc gcacatctcg cgcaggttgc
    13081 ggtgcgtcgc gctgacgacg gcgatgtcga ccgggatcga cttgtggctg cccagcggcg
    13141 tgacgcagcg ctcctgcagc acgcgcagca ggcgcgcctg cagcgacagc ggcatgtcgc
    13201 cgatctcgtc gaggaacagc gtgccgccgt tggcctgcag gatgcggccg gtcgcgccct
    13261 tgcggcgggc gccggtgaac gcgccgtcct cgtagccgaa cagctcggcc tcgatgagcg
    13321 actcggggat cgacgcgcag ttgaccgcca cgaagggctg gcgcgcgcgg ttggaatcct
    13381 ggtggacggc gcgcgcgagc agctccttgc cggtgcccgt ctcgccgagg atcagcagcg
    13441 ggatctcctt gttcagcacg cgctgcacct tggtgacgac ggcctcgacc tgcgcgtcgc
    13501 cggtgctcag gtaatgcagg ccggacaggc aggcgcgtcg cgcggactcg tccgccgcgg
    13561 cgcgatgcgg cacgctcgcg gtggtggcgg cggccggcgc ggcgtcgtga tcggcgcgcg
    13621 tcgcgggcgc gccctccaga cccgccatca ccgagcgcag cggtccgttg aagcgcgcgc
    13681 tcgcatggaa ctggcggcca ttggacatcg tcagccgcat cggcagcggg atgggcgcgc
    13741 ggaagtggtc gatcacggcg ccgatcgtcg tctcgaacag gctcgtgatc gtgtgcatgc
    13801 gcagcgcggc gccgctgacg tcgagctggt cgagcgcgct gcggttggcg cccaggatgc
    13861 ggccgtcgcc gcccacgacg atgatgccct ccagcaaagt gccgatgaac tcgggccgca
    13921 ggtggaagtg cagccgcagc cggttgccgt agtcctcgga cagccagtgg ttctcgatca
    13981 tgcgcgccga catgcgcacg agccccatcg tgtgctggtg gtacgacacc tggtcgccgg
    14041 tgacgtcgag cacgccaagc atgttgccgc gcgggtcgac gatgggcgcc gcggagcacg
    14101 tgaggaagct gttggcgtgg atgaagtgct cgcccgcgtg cacgagcgtg ggccgctcct
    14161 cgacgagcgc cgtcccgatc gcgttcgtgc ccttggactg ctcggcccag ttgacgcctg
    14221 gcgccagcgc caccttgccg gcgcgcgcga ggaattcgtc gtcgccgatc gagtgcagga
    14281 tcgtgccctg ggcgtcggtc agcacgatca tgctctcggt gttgacgatc tgctcgaaca
    14341 gcatctccat caccggcgcc gcgtgcgtca gcaggcgctg gttgcgctcg cgcgccacga
    14401 cgaggtcgcc gcggatcatc ggctcgaagt cgggccgctc gatgcgcgtc agtccgagcg
    14461 ccgtgcagcg caggtgcgac tgctcgatga gctcggcgcg cgcggtcgcg tcggcggcgg
    14521 tgggcgtcgt ccacccccca gggatgggaa gggcgcggag ggatgctgac atgggtcgct
    14581 tcctctgttc ctgcccgacg gcaggttgtc tctcgttgtg ccgtcgagct gcggaggccg
    14641 aggggccgcc gtcgtgccgc ggactgttcg atcctggtac agctcgcatg tcacagcgtc
    14701 ggggccaacc ctgacgaacg cctgacggcg cgggccgccg gtggcattcc aggatagcac
    14761 tcggccctgt cgattcccag ggacggagtc cccggggacc ggtgcgcgcg tccagttgct
    14821 tgcgaattcg caagaaacat tcccggcctg gagcaccgct ttttttggca cgagctttgc
    14881 agaccgtctg cccgttccag atccctttgc aacgacaccg gagacagcca tgcgacgtca
    14941 cgcccaccct gcccttcccc cgacgaacca gcgcccgcga cgcctcgccg ccgccgtgcg
    15001 catcggctcg ctcctgctca cgctgggcgc ctgtgtcgtc gtcgccgcgc acggcaacgt
    15061 gacgccccag cccgtcgaca ccgccggcct cccagccgtg ggccaggact ggctgagcgt
    15121 gaatccgtac tcgggcaacc cgaaggcggt ggagatcggc tcgtccggct tcaaccagaa
    15181 ctgcgcgcgc tgccatgggt tggaggcgat ctccggcggc atcgcgcccg acctgcggcg
    15241 cctggacaac gactgcgcgg gcatgaagga cccggccgcg cgcgacggct gcatcaagga
    15301 caacgacgag tacatgatca ccaccgtgcg ccacggccgc acccgcaacg gcgccgtcta
    15361 catgccgccg ttcgagggcg tgctgagcca ggaagcgatg tgggcgatca agacctacct
    15421 ggagtcgcgc cgcgagaagc cgctgcagga cgcgcaatga ccgccgcccg catcgaccgg
    15481 cgcggcgcgc tgcgcgcgct cggcgccgcg gcgctcgtcg catcgggcgg cgtgcgcgcg
    15541 gccgggacgg gcctggagga cgtcaaggcg cgcggcacgc tgaccgtggc cgtctaccgc
    15601 gagatgccgc cgttccacga caagggccag ggcatcgatg tcgacctcgc cgcggcgctc
    15661 gccggcgcgc ttggcgtcaa gctctcgctg atgccgtttc cggccggcga ggacatgaac
    15721 gacgacctgc gcaacatggt ctggaagggc cactacctgg gctacgggcc ggccgacgtg
    15781 atgctgcacg tcccggtgga gtcgccgctg atggacgcca atccgcaggt gagcatcttc
    15841 gccccgtact ggcgcgaccg cgtgatgatc gcccgcgacg tgtc
//
LOCUS       DATCRO010000196         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_373375, whole genome shotgun sequence.
ACCESSION   DATCRO010000196 DATCRO010000000
VERSION     DATCRO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373375"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VIN75_05215"
     CDS             complement(<1..1117)
                     /locus_tag="VIN75_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8973597.1"
                     /translation="MNDMTSFAFRIASLAAAAALAACAAVGPDYKQPPEALASQPAAG
                     KPFAEAPANAAPLPAHWWRLYHDPLLDKLVEQALAHNTDLRQAVANLERERAIEDETA
                     GARYPTIGVNGGPSFGHVSGLSLLQKGYEPPSTFNYSAGASLSYQVDLFGQIRRAIEA
                     SQASTQAAEAALDLVRVNVAAGTARAYAEACSTGLRLQIAQKSVALQQDAVNVTDRLQ
                     HAGRAGAIDANRARAQLQQLNAALPPLKAERQGALYRLATLTGALPQDFPREVADCTV
                     PPRVAGVLPVGDGAALLRRRPDIRQAERSLAASTARIGVATAELYPKVTLGLSGSSAG
                     HADDFGRKDTFAWSLGPLISWTIPNTGVAQARIAQAEA"
     gene            complement(1114..2055)
                     /locus_tag="VIN75_05220"
     CDS             complement(1114..2055)
                     /locus_tag="VIN75_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY8973598.1"
                     /translation="MKAARTSDDSNPWAQRLGRVLFTLAVVAAAVWAGLRLWDHYELA
                     PWTRDGRVRANVVQIAPDVSGLVTAVPIHDNQPVAAGALLFEVDRARYDLAVRQAQAA
                     LAAQRAVSAQAQRENARNAGLDELVSKESREQTRSRVEQMQAAVAQAEVALDSARLNL
                     QRAQVRAPADGLVTNLDLRQGSYAAAGRPVLALVDAQSFYVEGYFEETKLPRIHLGDR
                     VRVTPMGGGAVLEGSVESVAAGIADHDRSTSANLLPSVNPTFNWVRLAQRIPVRVKLD
                     PLPQGARLVAGQTVTVQVLENKPVQKTAATASTPQKG"
     gene            complement(2072..2278)
                     /locus_tag="VIN75_05225"
     CDS             complement(2072..2278)
                     /locus_tag="VIN75_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY8973599.1"
                     /translation="MIGEASFYGLYLPWIMVLALAALLVLWGVRRLLAATGAYRWVWH
                     PALFDMALYLLLLYALSRASAYLQ"
     gene            complement(2275..>4281)
                     /locus_tag="VIN75_05230"
     CDS             complement(2275..>4281)
                     /locus_tag="VIN75_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY8973600.1"
                     /translation="WALMTTYVVAHPLAGAVRSKAVYRFLGTLIGSTATVLLVPALSN
                     APELLTLVLALWVGLCLCISLFDRTPRSYVFMLAGYTAALIGFPSVQTPLALFDTAVA
                     RVEEIGLGIFCATLVHSLVLPAGLAPTVLGLLDRTLQDARKWLGDLFQPAGRGSAEPR
                     RLDDDRRRLAGDITQLRLLSTHVPFDTTHLRWTAGAIRAMQDRVAALTPALSAVEDRL
                     LALEQAEGVIAPDVAAVLAKAAQWLREEADASGDATARAVRLQELRRAIEVLGATPPE
                     AQPQTRWGRALRIGLAGRLEELVDGWTACAQLRQDIDEGLKGAAPLRRTAALGSRVLH
                     RDYGMALLSALAAVIAICLCGAFWILTGWPMGSAATMMAAVFCCFFATMDDPVPAIHG
                     FLKWTLWSIPISAVYVLVLMPTVQDFGMLVLICAPLFLLLGFYLPRPTHFMAAMALIF
                     GVCGTLAMHDTASSDLVSFLNSMIGQIVGVVVAARVTRLVRSVGADWSARRIQRATWR
                     ELGDMAASSQPQYAQSDAYAVRMLDRIGLLAPRIAQAGGTVEGVAANDALRDLRMGAD
                     IAVLQRVRGQLPLATSAALLGGIARFFRQRAEGRMNPRPPGLLPQIDEALGAVLDARG
                     TASSSSSSASRSAVTALVGLRRNLFPDAPPSLASTSLTSQGASA"
BASE COUNT          617 a         1430 c         1681 g          553 t
ORIGIN      
        1 cggcctctgc ctgcgcgata cgggcctgcg cgacgccggt gttgggaatg gtccaggaaa
       61 tcagcgggcc caggctccag gcgaaggtgt ccttgcggcc gaaatcgtcg gcatggcctg
      121 cggaagagcc cgagaggccc agcgtgacct tcggatagag ctccgcagtc gccacgccga
      181 tgcgtgcggt cgatgcggcc aggctgcgtt cggcctggcg gatgtcgggc cggcggcgca
      241 gcagcgcggc gccgtcgcca acgggcagca cgccggccac gcgcggcggc acggtgcagt
      301 cggccacctc gcgcgggaag tcctgcggca gcgcgccggt gagcgtggcg aggcgataca
      361 gcgcgccctg gcgctcggcc ttcagcggcg gcagcgcggc gttgagctgc tgcagctgcg
      421 cgcgtgcgcg gttcgcgtcg atggcgccgg cgcggcccgc gtgctgcagg cggtcggtga
      481 cgttgacggc gtcctgctgc agcgcgacgg acttctgcgc gatctgcagc cgcaggccgg
      541 tggagcaggc ttcggcatac gcgcgcgcag tgcctgccgc cacgttcacg cgcaccaggt
      601 cgagcgcggc ttcggcggcc tgcgtgctgg cctgcgaggc ctcgatggcg cggcggatct
      661 ggccgaacag gtcgacctgg taggagagcg acgcgcccgc gctgtagttg aaggtgctcg
      721 gcggctcgta gcccttctgc agcagcgaca ggcccgagac gtggccgaac gaagggccgc
      781 cgttcacgcc gatggtgggg tacctggcgc cggcggtctc gtcctcgatg gcgcgctcgc
      841 gctcgaggtt cgcgacggcc tggcgcaggt cggtgttgtg cgcgagggcc tgctcgacca
      901 gcttgtcgag cagcgggtcg tggtacaggc gccaccagtg ggcgggcagc ggcgccgcgt
      961 tggcgggcgc ctcggcgaag ggcttgcccg cggcgggctg gctggccagc gcctcgggcg
     1021 gctgcttgta gtcggggccg accgccgcgc aggccgcgag cgcggcggcg gcggccagcg
     1081 atgcgatgcg gaaagcgaag ctggtcatgt cgttcatccc ttctgcggcg tggaagccgt
     1141 tgccgccgtc ttctgcacgg gcttgttctc cagcacctgc accgtgaccg tctggcccgc
     1201 gacgaggcgc gcaccctgcg gcagcggatc gagcttgacg cgcaccggaa tgcgctgcgc
     1261 gagccgcacc cagttgaagg tcgggttcac gctgggcagc aggttggcgc tggtggagcg
     1321 gtcgtggtcg gcgatgccgg cggccacgct ctcgaccgag ccttcgagca cggcgccgcc
     1381 gcccatgggc gtgacgcgca cgcggtcgcc gaggtggatg cgcggcagct tggtctcttc
     1441 gaaatagcct tcgacgtaga acgactgcgc atcgaccagc gcgagcaccg gccggcccgc
     1501 cgcggcatag ctgccctggc gcaggtcgag gttggtgacc aggccgtcgg ccggcgcgcg
     1561 cacctgggcg cgctgaaggt tgaggcgtgc cgagtcgagc gccacttcgg cctgggcgac
     1621 cgcggcctgc atctgctcga cgcgcgagcg ggtctgttcg cgcgactcct tcgacaccag
     1681 ttcgtcgagg ccggcattgc gcgcgttctc tcgctgggcc tgtgcgctca cggcacgctg
     1741 cgcggcgagc gcggcctgcg cctggcgcac ggcgaggtcg tagcgcgcgc ggtccacttc
     1801 gaagagcagc gcgccggcgg ccaccggctg gttgtcgtgg atgggcactg ccgtgaccag
     1861 gcccgagacg tcgggcgcaa tctgcacgac gttggcgcgc acgcggccgt cgcgcgtcca
     1921 cggcgcgagt tcgtagtggt cccacaggcg caggccggcc cacacggcgg cggcgaccac
     1981 ggccagggtg aagagaacgc ggcccaggcg ctgggcccac ggattgctgt cgtcggaagt
     2041 tcgggcggct ttcattggaa tctcgttgtt gtcattggag gtaggcgctg gcgcggctga
     2101 gcgcgtagag cagcagcagg tagagcgcca tgtcgaagag ggccggatgc cacacccagc
     2161 ggtaggcgcc ggtggccgcg agcaggcggc gcacgcccca cagcacgagc agtgccgcga
     2221 gcgcgagcac catgatccag ggcaggtaca ggccgtagaa gctggcttcg ccgatcatgc
     2281 ggaagctcct tgcgaagtga gggacgtgct cgcgagcgag ggcggcgcgt cgggaaagag
     2341 attgcggcgc aggcccacca gtgcggtgac ggccgagcgc gaggctgacg aagaagacga
     2401 cgacgcggtg ccgcgcgcat cgagcacggc gccgagcgcc tcgtcgatct gcggcagcag
     2461 gccgggcggg cgcgggttca tgcggccttc cgcgcgctgg cggaagaagc gcgcgatgcc
     2521 gccgagcagc gcggccgagg tggccagcgg cagctggccg cgcacgcgct gcagcacggc
     2581 gatgtcggcg cccatgcgca ggtcgcgcag cgcgtcgttg gcggccacgc cctcgacagt
     2641 gccgccggcc tgcgcgatgc gcggcgcgag caggccgatg cggtcgagca tgcgcacggc
     2701 ataggcgtcg ctctgcgcgt actgcggctg cgaagacgcg gccatgtcgc ccagctcgcg
     2761 ccaggtggcg cgctggatgc gccgcgcgct ccagtcggcg cccaccgagc gcacgagacg
     2821 cgtgacgcgc gccgcgacga ccacgccgac gatctggccg atcatgctgt tgagaaagct
     2881 caccaggtcg gagctggccg tgtcgtgcat cgccagcgtg ccgcacacgc cgaagatcag
     2941 cgccatcgcg gccatgaagt gcgtggggcg cggcaggtag aagcccagca gcaggaagag
     3001 cggcgcgcag atgagcacca gcatgccgaa gtcctgcacc gtgggcatga gcaccagcac
     3061 gtagacggcc gagatcggga tcgaccacag cgtccacttc aggaagccgt ggatcgccgg
     3121 cacggggtcg tccatcgtcg cgaagaagca gcagaagacg gcggccatca tggtggcggc
     3181 cgagcccatg ggccagccgg tgaggatcca gaaggcgccg cacaggcaga tggcgatgac
     3241 ggcggccagt gccgacagca gcgccatgcc gtagtcgcgg tgcagcacgc ggctgccgag
     3301 cgcggccgtg cggcgcagcg gcgcggcgcc cttcaggcct tcgtcgatgt cctggcgcag
     3361 ctgggcgcag gcggtccagc cgtcgacgag ctcttcgagg cggcctgcga ggccgatgcg
     3421 aagggcgcgg ccccagcggg tctgcggctg tgcctcgggc ggcgtggcgc ccagaacctc
     3481 gatggcacgg cgcagctcct gcaggcgcac ggcgcgggcc gtcgcgtcgc cggaggcatc
     3541 ggcctcctcg cgcagccact gcgcggcctt ggcaagcacg gcggccacgt cgggcgcgat
     3601 gacgccttcg gcctgctcca gcgccagcag gcggtcttcg acggccgaga gcgccggcgt
     3661 gagcgcggcg acgcggtcct gcatcgcgcg gatggcaccg gccgtccagc gcaggtgcgt
     3721 ggtgtcgaag ggaacgtggg tggagagcag gcgcagctgc gtgatgtcgc cggcgaggcg
     3781 gcggcggtcg tcgtcgaggc gccggggctc ggcgctgccg cggccggcgg gctggaagag
     3841 atcgcccagc cacttgcgcg cgtcctgcag cgtgcggtcg agcaggccca gcacggtcgg
     3901 cgcgagcccc gcgggcagca cgaggctgtg gacgagcgtg gcgcagaaga tgcccaggcc
     3961 gatctcttcg acgcgcgcca cggcggtgtc gaacagcgcg agcggcgtct gcaccgacgg
     4021 gaagccgatc agcgccgccg tgtagcccgc gagcatgaag acgtaggagc gcggcgtgcg
     4081 gtcgaagagc gagatgcaca ggcacagccc cacccacagc gcgagcacca gcgtgagcag
     4141 ctcgggcgcg ttggacagcg ccggcaccag cagcacggtc gcggtgctgc cgatgagcgt
     4201 gccgaggaaa cggtagaccg ccttggagcg caccgcgccg gccagcggat gggcgacgac
     4261 gtaggtggtc atgagcgccc a
//
LOCUS       DATCRO010000197        11083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_69663, whole genome shotgun sequence.
ACCESSION   DATCRO010000197 DATCRO010000000
VERSION     DATCRO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69663"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VIN75_05235"
     CDS             complement(<1..546)
                     /locus_tag="VIN75_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8973601.1"
                     /translation="MITSAHAQPLNVLHWWTSDSERQAADRLVQQLAAQGVEWHDDAI
                     PGGGGGAAIKVLTGRVLAGEPPDVAQVIGRTLTDWAGLGLVLPLDDVAQRGHWRQVMF
                     PVVLETVSRNGHVMAAPLGVHRINTLLVNHHVLEQLRLKAPTTWAEMDAAAKVMRAHQ
                     VTPLAWSDEPWQIATVFETVML"
     gene            890..2146
                     /locus_tag="VIN75_05240"
     CDS             890..2146
                     /locus_tag="VIN75_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8973602.1"
                     /translation="MITLLRTTTLAAAALACAVPALAGTLTIESWRTDDKQLWDTVLL
                     PAFAKSHPGITVKFVATAPPEYNSVVAARLASGSAGDLITCRPFDVSLDLYKKGHLEK
                     LNGNPALNNFPDSAKVAWQTDDGKDTYCVPMASVIHGFFYNKKIFGELGLKPPATEAD
                     FFADLDKIKAGGKYVPLDLGTADQWDTSQVVFTGIGPNYWHGEDGRKALIAGKAKFTD
                     PQFVDVWQTMAKYPPYLAKGYQAQKYGDTQTLFAAGKAAVVPAGSWDIAFYQQQGGVD
                     FGAFPPPVRKAGDACYISDHTDIGMGINTASKNKADAKVFLDWIASREFADLYTNKVT
                     GFFSLSKFPVQVQNPVAQEMVGWRAHCKSTIRLNAQIMSRGEPSMENELWNVNSQVLN
                     GKMTPQQAGERIQSGFAKWYHAPASK"
     gene            2157..3071
                     /locus_tag="VIN75_05245"
     CDS             2157..3071
                     /locus_tag="VIN75_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8973603.1"
                     /translation="MKQKAGWPAIVFLAPALIVYTAFSAWPLLATLRLGLYATDDTGH
                     VAFAGLANFQKLLFDEHWWGAFRNAMVNNLSFFAIHTLVQNPVALLLAALLSLRGVRG
                     AAAYRTVLFLPTLLSVVIIGFAWQLILSPLWGVSEKLMDLVGLGDHFGPWLGQEDTAL
                     RTIALISVWQFVGVPLMLIYAALIAIPEEVVEAAQVEGAGPWRIFWRIRLPLILPTLG
                     LVNILTFVGNFNAFDLVYAVKGALAGPNFASDLLGTLFYRTFFGYQSEVGSATMGAAL
                     ATLMFLVILGGVGLYFFVIQRRLQRVAG"
     gene            3068..3925
                     /locus_tag="VIN75_05250"
     CDS             3068..3925
                     /locus_tag="VIN75_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8973604.1"
                     /translation="MTATASPIRRADAISRTALVHLTLGGYCVLVLGPILLVILNSFK
                     TRAGIFDAPLGLPTHDTLSLAGYARVLGDAAMGRMFWNSFVVTGLSLVLILLLGAMAA
                     WALTEYRFFGNRLLALFLAFGIMVPIRLGSVAILQEMVALDLVNTLTALVLVYIAQGL
                     PLAVMILSEFVAQVPQELKDAARCDGVGEFRIFFQVVLPLIRPAVATVAVFTMVPIWN
                     DLWFPLILAPSEQTQTITLGVQQFVGQFATDWNAVLAALSLAVLPILVLYAVFSRQLI
                     RGITAGAVK"
     gene            3933..5048
                     /gene="ugpC"
                     /locus_tag="VIN75_05255"
     CDS             3933..5048
                     /gene="ugpC"
                     /locus_tag="VIN75_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8973605.1"
                     /translation="MSTVRLSNVSKSYGSNRVLHDIDLEIADGELVVLVGPSGCGKST
                     LLRMICGLEPITGGELRIDDQVVNELPPARRGIAMVFQSYALYPHMTVRGNMAFGLQI
                     AGASRADRDARILAAARALHIDHLLDRRPRELSGGQRQRVAIGRAIVRQPKLFLFDEP
                     LSNLDAALRVKTRIELARLHRELAATMVYVTHDQVEAMTLGDRIVVMHEGRVQQAGSP
                     LELYEHPANRFVATFIGSPTINLVPATTQACSAGGIRVEVHAGCEVDAPIDGSALAPG
                     QRVELGIRPEHLSVADGAGDPSRECLRFGGEVTLVEQLGESHLVYVRCADGLELVARA
                     PGHTRLRLGDRIGLEADPKGLHVFDANGRACRRTAPD"
     gene            complement(5281..8943)
                     /locus_tag="VIN75_05260"
     CDS             complement(5281..8943)
                     /locus_tag="VIN75_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8973606.1"
                     /translation="MNPTDPTPASSSRWQFWIDRGGTFTDIVAKKPDGTLVTHKLLSE
                     NPEQYRDAAVAGIRHLLGLRPGEPVTPALVECVKMGTTVATNALLERKGEKTLLVTTR
                     GFRDALRIAYQDRPRLFDRHIQLPELLYSRVIEAEERVSAQGEVLQALDESALRAELQ
                     AAFDAGLRSVAVVFLHGYRFTQHEAAAARIAREVGFTQVSASHATSPLMKLVSRGDTT
                     VVDAYLSPILRRYVEQVAGEMPGVKLFFMQSSGGLADAHAFQGKDAILSGPAGGIVGM
                     VRTAQLADFGHVIGFDMGGTSTDVSHFAGEFERAFETQVAGVRMRAPMMSIHTVAAGG
                     GSILGFDGARLRAGPHSAGANPGPASYRRGGPLAVTDANVLLGKIQPRWFPQVFGKAA
                     NEPLDREAVVAAFDRMAADVEKSTGTKRSPEELAEGFLDIAVASMANAIKKISVARGY
                     DVTRYTLQCFGGAGGQHACLVADALGMSTVFVHPLAGVLSAYGMGLADQGVMRQAAIE
                     QPLADAALPAVRARLDTLADEAVAEIRRQGAGTGEIRVLRRAHLRYTGTDSALVVDFG
                     DGSVATLTAGFETAYRRRYAFLMQGRALIVEAVSVEAIAAGDAPAEPRVEVGPATPAP
                     VTERVRMYGEGRWWDAALVVRETLRPGHTVDGPAIIAEKNATTVVEPGWQARVTGLDH
                     LVLQRVAPRAARVAIGTSVDPVMLEVFNNAFMNIAEQMGLQLQNTAYSVNIKERLDFS
                     CALFDASGNLIANAPHMPVHLGSMSESIKTVMARNAGRMEPGDVYVLNDPYHGGTHLP
                     DITVVTPVFDEAGKDILFFVASRGHHADVGGVTPGSMPPFSTRIDEEGVQIDNVKLVE
                     RGRLREAEMLALLESGDHPARNPAQNIADLKAQIAANEKGVQELRKMVAQYGLDVVQA
                     YMGHVQDNAEESVRRVITRLKDGAFTLPLDNGARIAVDIKVDAANRRARIDFTGTSAQ
                     LANNFNAPTAVCMAAVLYVFRTLVGDDIPLNAGCLKPLDVIIPPHSMLNPDPPASVVA
                     GNVETSTCITNALYGALGVMAAGQCTMNNFTFGNERHQYYETISGGSGAGALIDERGE
                     VVGGFPGTSVVQTHMTNSRLTDPEVLEFRFPVRVDSYEIRPGSGGAGQWRGGDGGTRR
                     IRFLEAMTASILSNGRHHGAFGMGGGQAGQVGRNSVVRADGRVEALDHIGSAQMQPGD
                     VFVVETPGGGGFGRAD"
     gene            complement(8949..9716)
                     /locus_tag="VIN75_05265"
     CDS             complement(8949..9716)
                     /locus_tag="VIN75_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEY8973607.1"
                     /translation="MPIHHDDPAALRLACRSGEFDRPTAGHAPGRVQANLTIVPQADA
                     FDFLLFCQRNPQACPIVEVLAPGARAVRAAPGSDIATDLPGYRVYRDGELVDERADVA
                     ALWRPDLVAFLTGCSFSFESALEDAGIPLRHVRQQRNVAMFRTTRPCVPAGRFAGELV
                     VSMRPIRSRDVARVIEICSRFPQAHGAPVHVGNPHALGITDPMRPDYGDAVDIQGDEV
                     PVFWACGVTTQWVVQRSGLPFCITHAPGKMFVTDLMG"
     gene            complement(9773..10702)
                     /locus_tag="VIN75_05270"
     CDS             complement(9773..10702)
                     /locus_tag="VIN75_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973608.1"
                     /translation="MNQRFVEAFYWVATLKSMTRAAEKLFMTQSAISSRVSALEEELG
                     TLLIDRRDRQQFALTNAGMRFLDYAERLMAIHRQMRDELGTEEAGGVHLLRVGAIESV
                     LHTWLIPLLQHLRSVHPKLQLELTVEITPVLAEAVKRGTLDLVFAAQSVSAPDIRTRP
                     LPTMEMVFVGAKSLKRRTPYTLADIVAHEVMTFQRGSQPHLALLEQLRGEGLEPPRLH
                     AVSSIPAMVHLIEAGFGIATLPRIAAESLVRQHEIAILPCATTLSPLPVLASYRHDPG
                     SSSLTSVIQDALKFIDAFGGTAGKARLAQKKNR"
     gene            complement(10757..>11083)
                     /locus_tag="VIN75_05275"
     CDS             complement(10757..>11083)
                     /locus_tag="VIN75_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973609.1"
                     /translation="ALFQSWAESAVAGGAAPKGLEQAFAAHVEISPSMWSQIKSSRPI
                     GDKLARQIEQHCGKPAGWLDEEREAAGLTPGEQQFLALALQAWRATNADGRKMLKSQM
                     KQLAQG"
BASE COUNT         1569 a         3883 c         3897 g         1734 t
ORIGIN      
        1 gagcatcacc gtctcgaaca cggtggcgat ctgccacggc tcgtcgctcc atgcgagcgg
       61 cgtcacctgg tgcgcgcgca tgaccttggc cgcggcgtcc atctcggccc atgtcgtcgg
      121 ggctttcagg cgcagctgtt cgagcacgtg gtggttgacc agcagcgtgt tgatgcggtg
      181 cacgcccagg ggcgcggcca tcacgtggcc gttgcggctg accgtctcga gcaccaccgg
      241 gaacatcacc tggcgccagt gcccgcgctg cgcgacgtcg tccagcggca gcacgaggcc
      301 caggcccgcc cagtcggtga gcgtgcggcc gatcacctgc gcgacatccg gcggctcgcc
      361 ggcgagcacg cggcccgtca gcaccttgat ggccgcgccg ccgccgccgc cggggatcgc
      421 gtcgtcgtgc cattcgacgc cttgcgcggc gagctgctgc acgaggcggt cggcggcctg
      481 gcgttcgctg tcggacgtcc accagtgcag gacgttcagg ggctgggcgt gcgcgctggt
      541 gatcatgcaa agggccagga gagcgcccag tatcgacgcc gcgccggcgc gcgcgggccg
      601 gcgcgcggct gtcgaaagcc tgcgctttgc aatgttttgt aagcccggcg cgggatgtcg
      661 catgcggcct gtgcggtgac ggtgagcgtg gacgtaatga tgcgtaagcc gctgtcgctg
      721 gcaatgctgg aaaccacctt gcgaatcaag cacttgcgcg ccgcgcgatg cggcccgcgc
      781 gacgctcgcg ttacgcggcg accgatgcta acttgcgccc gcctcgtcgc cgcacgtcga
      841 caggcggact gcacgattca agacccgaca cctcccaagg aaccgaccga tgatcacgct
      901 gctccgcacc accaccctgg ccgccgccgc gctggcctgc gccgttcctg cactggccgg
      961 caccctcacc atcgagagct ggcgcaccga cgacaagcag ctgtgggaca ccgtgctgct
     1021 gcctgcgttc gcgaaaagcc acccgggcat caccgtgaag ttcgtggcca ccgcgccgcc
     1081 ggaatacaac tcggtcgtcg ccgcgcgcct ggcctcgggc agcgcgggcg acctcatcac
     1141 gtgccgtccg ttcgacgtct cgctggacct gtacaagaag ggccacctcg agaagctcaa
     1201 cggcaacccc gcgctgaaca acttccccga ctctgcgaag gtcgcctggc agaccgatga
     1261 cggcaaggac acctactgcg tgcccatggc ctccgtcatc cacggcttct tctacaacaa
     1321 gaagatcttc ggcgagctgg gcctcaagcc gcccgccacc gaggccgact tcttcgcgga
     1381 cctcgacaag atcaaggccg gtggcaagta tgtgccgctc gacctgggca ccgccgacca
     1441 atgggacacc agccaggtcg tgttcaccgg catcgggccg aactactggc atggcgagga
     1501 cggccgcaag gcgctgatcg ccggcaaggc gaaattcacc gacccgcagt tcgtcgacgt
     1561 ctggcagacg atggccaagt acccgccgta cctggccaag ggctaccagg cgcagaagta
     1621 cggcgacacg cagacgctgt tcgccgccgg caaggcggcg gtcgtgcccg ccgggtcgtg
     1681 ggacatcgcg ttctaccagc agcagggcgg cgtcgacttc ggcgcgttcc cgccgccggt
     1741 gcgcaaggcc ggcgacgcct gctacatctc cgaccacacc gacatcggca tgggcatcaa
     1801 cacggccagc aagaacaagg cggacgccaa ggtgttcctc gactggatcg cgtccaggga
     1861 attcgccgac ctgtacacga acaaggtcac cggcttcttc tcgttgtcga agttccccgt
     1921 ccaggtgcag aacccggtgg cgcaggagat ggtgggctgg cgcgcgcact gcaagtccac
     1981 gatccgcctg aatgcgcaga tcatgagccg cggcgagccc agcatggaga acgagctctg
     2041 gaacgtgaat tcgcaggtgc tcaatggaaa gatgacgccg cagcaggccg gcgaacgcat
     2101 ccagtccggc ttcgccaagt ggtaccacgc gccggcctcg aagtgacgtc acgggcatga
     2161 agcagaaggc cggctggccg gcgatcgtct tcctcgcgcc cgcgctgatc gtctacaccg
     2221 cgttcagcgc gtggccgctg ctggccacgc tgcggctggg cctgtatgcc accgacgaca
     2281 cgggccacgt cgcgttcgcg ggactcgcga acttccagaa gctgctgttc gacgagcact
     2341 ggtggggggc gttccgcaac gcgatggtca acaacctctc gttcttcgcg atccacaccc
     2401 tggtgcagaa cccggtggcg ctgctgctgg cggcgctgct gtcgctgcgc ggcgtgcgcg
     2461 gcgcggcggc gtaccgcacg gtgctgttcc tgccgacgct gctgtcggtg gtgatcatcg
     2521 gcttcgcgtg gcagctgatc ctgtcgccgc tatggggcgt cagcgagaag ctgatggacc
     2581 tcgtcggcct gggcgatcac ttcggcccct ggctgggcca ggaggatacg gccctgcgca
     2641 cgatcgcgct gatctccgtg tggcagttcg tgggcgtgcc gctgatgctg atctacgcgg
     2701 cgctgatcgc gatccccgag gaggtggtcg aggccgcgca ggtcgagggc gcggggccgt
     2761 ggcgcatctt ctggcgcatc cgcctgccgc tgatcctgcc gacgctgggc ctcgtcaaca
     2821 tcctgacctt cgtcggcaac ttcaacgcgt tcgacctcgt ctacgccgtc aagggcgcgc
     2881 tggccgggcc caacttcgcg tcggacctgc tgggcacgct gttctaccgc acgttcttcg
     2941 gctaccagtc cgaggtcggc agcgccacga tgggcgcggc gctggccacg ctgatgttcc
     3001 tcgtcatcct cggcggcgtc gggctgtact tcttcgtgat ccagcggcgg ctgcagcggg
     3061 tggccggatg acggcgaccg cttctccgat ccggcgtgcc gacgccatca gccgaacggc
     3121 gctcgtgcac ctgacgctcg gcggctactg cgtgctggtg ctcgggccga tcctgctggt
     3181 gatcctgaat tcgttcaaga cgcgcgcggg catcttcgac gcgccgctcg gcttgcccac
     3241 gcacgacacg ctgagcctgg ccggctacgc ccgcgtgctg ggggatgcgg cgatgggccg
     3301 catgttctgg aacagcttcg tcgtcaccgg cctctcgctc gtgctgatcc tgctgctggg
     3361 cgcgatggcc gcgtgggcgc tcaccgagta ccgcttcttc ggcaaccggc tgctcgcgct
     3421 gttcctcgcg ttcggcatca tggtgccgat ccgcctgggc tcggtggcga tcctgcagga
     3481 gatggtggcg ctggacctcg tcaacacgct cacggcgctg gtgctggtct acatcgcgca
     3541 ggggctgccg ctggcggtga tgatcctgtc ggagttcgtc gcgcaggtgc cgcaggaact
     3601 caaggacgcc gcgcgctgcg acggcgtcgg cgagttccgc atcttcttcc aggtggtgct
     3661 gccgctgatc cggccggcgg tcgcgacggt ggccgtgttc acgatggtgc cgatctggaa
     3721 cgacctgtgg ttcccgctga tcctcgcgcc gtccgagcag acgcagacga tcacgctggg
     3781 cgtgcagcag ttcgtcggcc agttcgccac cgactggaac gccgtgctgg ccgcgctgtc
     3841 gctggccgtg ctgcccatcc tcgtgctgta cgcggtgttc tcgcgccagc tgatccgcgg
     3901 catcaccgcg ggcgccgtca agtaggtctc ccatgtccac ggtccgactc tccaacgtct
     3961 ccaagagcta cggcagcaac cgcgtgctgc acgacatcga cctcgagatc gccgacggcg
     4021 agctggtggt gctggtcggc cccagcggct gcggcaagtc cacgctgctg cgcatgatct
     4081 gcgggctcga gccgatcacc ggcggcgagc tgcgcatcga cgaccaggtc gtcaacgagc
     4141 tgccgcctgc gcgccgcggc atcgcgatgg tgttccagag ctacgcgctg tacccgcaca
     4201 tgacggtgcg cggcaacatg gccttcggcc tgcagatcgc gggcgcctcg cgcgccgacc
     4261 gcgacgcgcg catcctcgcc gcggccaggg cgctgcacat cgaccacctg ctggaccgcc
     4321 ggccgcgcga gctgtccggc ggccagcgcc agcgcgtggc catcggccgg gccatcgtgc
     4381 gccagcccaa gctgttcctg ttcgacgagc cgctgtccaa cctcgacgcg gcgctgcgcg
     4441 tgaagacccg catcgagctt gcccgcctgc accgtgagct ggccgccacc atggtctacg
     4501 tcacgcacga ccaggtcgag gcgatgacgc tgggcgaccg catcgtcgtc atgcacgagg
     4561 gccgcgtgca gcaggccggc tcgccgctgg agctgtacga gcatcccgcc aaccgtttcg
     4621 tcgccacctt catcggctcg ccgacgatca acctcgtgcc cgcgaccacg caggcctgct
     4681 cggccggcgg catccgcgtc gaggtgcatg ccggctgcga ggtcgacgcc ccgatcgacg
     4741 gcagcgcgct cgcgcccggc cagcgcgtcg agctgggcat ccgtcccgag cacctgtccg
     4801 tcgccgacgg cgccggcgac ccgtcacgcg aatgcctgcg cttcggcggc gaggtcacgc
     4861 tggtcgagca gctgggagaa tcgcacctgg tctatgtgcg ctgcgcggac ggcctggagc
     4921 tggtcgcgcg cgcgcccggc cacacgcgcc tgcgcctggg cgaccggatc gggctggagg
     4981 cggacccgaa ggggctgcat gtgttcgatg cgaacgggcg ggcgtgtcga aggacggccc
     5041 cggactgagc gtcgccgcgc cagaaactct ggcgcgccga gccgttgccg aaggggtctg
     5101 gccttcccga gaatcgcgcc gcgatgccgc gatatcgcgc cgtgccgatc gcgattcgag
     5161 gacaggcctg accccgattc gctgacgttc ggggtcaggc ctgtccaccc tccgcggcct
     5221 acgtggtcgc ttcgaccagc atccttgcgc ggagtgcgga aaggccagac ccctcggcct
     5281 tcagtccgcg cgtccgaacc cgccgccccc cggcgtctcg accacgaaca catcgcccgg
     5341 ctgcatctgc gccgagccga tgtggtcgag cgcctcgacg cggccatcgg cccgcacgac
     5401 cgaattgcgg ccgacctgcc cggcctgccc gccgcccatg ccgaacgcgc cgtggtggcg
     5461 gccgttgctg aggatggacg cggtcatcgc ctcgaggaag cggatgcggc gggtgccgcc
     5521 gtcgccgccg cgccactggc cggcgccgcc ggagccgggg cggatctcgt agctgtccac
     5581 gcgcaccggg aagcggaact ccagcacctc ggggtcggtc aggcgcgagt tggtcatgtg
     5641 cgtctgcacg acgctggtgc cggggaagcc gccgacgacc tcgccgcgct cgtcgatcag
     5701 cgcgccggcg cccgagccgc ccgagatcgt ctcgtagtac tggtggcgct cgttgccgaa
     5761 ggtgaagttg ttcatcgtgc actggcccgc ggccatcacg cccagcgcgc cgtacagcgc
     5821 gttggtgatg caggtggacg tctcgacgtt gcccgccacc accgaggccg gcgggtcggg
     5881 gttgagcatc gagtgcggcg ggatgatgac gtccagcggc ttcaggcagc cggcgttcag
     5941 cgggatgtcg tcgcccacca gcgtgcggaa cacgtacagc acggcggcca tgcagaccgc
     6001 ggtcggcgca ttgaagttgt tggcgagctg ggcggacgtg ccggtgaagt cgatcctcgc
     6061 gcggcggttg gcggcgtcga ccttgatgtc gacggcgatg cgtgccccgt tgtccagcgg
     6121 cagcgtgaac gccccgtcct tgagccgcgt gatgacgcgc cgcaccgact cctcggcgtt
     6181 gtcctgcacg tggcccatgt aggcctgcac cacgtccagc ccgtactgcg ccaccatctt
     6241 gcgcagctcc tgcacgccct tctcgttggc ggcgatctgc gccttcaggt cggcgatgtt
     6301 ctgggccggg ttgcgcgcgg ggtggtcgcc gctttccagc agcgccagca tctcggcctc
     6361 gcgcaggcgt ccgcgctcga ccagcttgac gttgtcgatc tgcacgccct cctcgtcgat
     6421 gcgcgtggag aacggcggca tcgagcccgg cgtgacgccg cccacgtccg catggtggcc
     6481 gcgcgaggcg acgaagaaca ggatgtcctt gccggcttca tcgaacaccg gcgtgacgac
     6541 cgtgatgtcg ggcaggtgcg tgccgccatg gtacgggtcg ttgagcacgt agacgtcgcc
     6601 cggttccatc cggccggcgt tgcgcgccat caccgtcttg atcgattcgc tcatcgatcc
     6661 caggtgcacc ggcatgtgcg gcgcattcgc gatgaggttg ccgctcgcgt cgaacagcgc
     6721 gcaggagaag tccagccgct ccttgatgtt gaccgagtag gcggtgttct gcagctgcag
     6781 gcccatctgc tcggcgatgt tcatgaaggc gttgttgaac acctccagca tgaccgggtc
     6841 gacgctggtg ccgatggcca cgcgcgcggc ccgcggcgcc acgcgctgca gcaccaggtg
     6901 atccaggccc gtcacgcggg cctgccagcc gggctcgacg acggtggtcg cgttcttctc
     6961 cgcgatgatg gccgggccat cgaccgtgtg gccggggcgc agcgtctcgc gcacgacgag
     7021 cgccgcatcc caccagcgcc cctcgccgta catgcgcacg cgctcggtga cgggcgcggg
     7081 ggtggcgggg ccgacctcga cgcgcggctc cgcgggcgcg tcgccggcgg cgatcgcctc
     7141 gacggacacc gcctccacga tcaacgcgcg cccctgcatc aggaacgcat agcgccgccg
     7201 gtaggccgtc tcgaagcccg ccgtgagcgt ggcgacgctg ccgtcgccga agtccacgac
     7261 cagcgccgag tccgtgcccg tgtagcgcag gtgcgcgcgg cgcagcacgc ggatctcgcc
     7321 ggtgcccgcg ccctggcggc ggatctcggc gacggcctcg tcggccagcg tgtccagccg
     7381 ggcgcggacg gcgggcagcg cggcgtcggc cagcggctgc tcgatcgcgg cctggcgcat
     7441 cacgccctgg tcggccaggc ccatgccgta ggccgacagc acgccggcca gcgggtgcac
     7501 gaagacggtg gacatgccca gcgcgtcggc caccaggcac gcgtgctggc cgccggcgcc
     7561 gccgaagcac tgcagcgtgt agcgcgtgac gtcgtagccg cgcgccaccg agatcttctt
     7621 gatcgcattg gccatgctgg cgacggcgat gtcgaggaag ccctcggcca gttcctcggg
     7681 cgagcgcttc gtgccggtgc tcttctcgac gtccgccgcc atgcggtcga acgcggcgac
     7741 gaccgcctcc cgatcgagcg gctcgttggc ggccttgccg aacacctggg ggaaccacct
     7801 gggctggatc ttgcccagca gcacgttcgc gtcggtgacg gccagcgggc cgccgcggcg
     7861 gtagctggcc gggcccgggt tcgcgcccgc gctgtgcggg ccggcgcgca ggcgggcgcc
     7921 atcgaagccg aggatggacc cgccgccggc ggccacggtg tggatgctca tcatcggggc
     7981 gcgcatgcgc acgccggcga cctgcgtctc gaacgcgcgc tcgaactcgc cggcgaagtg
     8041 cgagacgtcg gtcgacgtgc cgcccatgtc gaagccgatc acgtggccga agtccgcgag
     8101 ctgcgccgtg cgcaccatgc cgacgatgcc gccggcgggg cccgacagga tggcgtcctt
     8161 gccctggaac gcgtgcgcgt cggcgaggcc gcccgaggac tgcatgaaga acagcttgac
     8221 gcccggcatc tcgccggcca cctgctcgac gtagcgccgc aggatcggcg acaggtaggc
     8281 gtccacgacg gtggtgtcgc cgcggctgac cagcttcatc agcgggctcg tcgcgtgcga
     8341 ggcgctgacc tgcgtgaagc cgacctcgcg ggcaatgcgc gcggcggccg cctcgtgctg
     8401 cgtgaagcgg tagccgtgca ggaagacgac cgccacgctg cgcaggccgg cgtcgaacgc
     8461 cgcctgcagc tcggcgcgca gggccgactc gtcgagcgcc tgcagcactt cgccttgtgc
     8521 gctcacgcgt tcctcggcct cgatgacgcg cgagtacagc agctcgggca gctggatgtg
     8581 gcggtcgaac aaccgcgggc ggtcctggta ggcgatgcgc agcgcgtcgc ggaagccgcg
     8641 ggtggtcacc agcagcgtct tctcaccctt gcgctcgagc agcgcgttgg tggccacggt
     8701 ggtgcccatc ttcacgcact cgacgagcgc cggcgtcacc ggctcgccgg gcctcaggcc
     8761 gagaagatgg cggatgccgg cgacggccgc gtcgcggtac tgctcggggt tctccgacag
     8821 caatttgtgc gtgacgagcg tgccatcggg cttcttggcg acgatgtcgg tgaaggtgcc
     8881 gccgcggtcg atccagaact gccagcgcga ggaggaggcg ggggtggggt cggtggggtt
     8941 cattcttgtc atcccatcag gtcggtgacg aacatcttgc caggggcgtg ggtgatgcag
     9001 aacggcaggc cgctgcgctg gaccacccat tgcgtggtca cgccgcaggc ccagaacacg
     9061 ggcacctcgt cgccctggat gtcgacggcg tcgccgtagt cgggccgcat cgggtcggtg
     9121 atccccagcg cgtgcgggtt gcccacgtgc acgggggcgc catgggcttg cggaaagcgc
     9181 gaacagatct cgatgacgcg cgcgacgtcc cggctgcgga tcggccgcat gctcaccacc
     9241 agctcgcccg cgaagcggcc ggccggcacg caggggcgcg tggtgcggaa catcgcgacg
     9301 ttgcgctgct ggcgcacgtg gcgcagcggg atgccggcgt cttccagcgc cgactcgaac
     9361 gagaagctgc agccggtcag gaaggcgacg aggtcggggc gccacagcgc ggcgacgtct
     9421 gcgcgctcgt ccacgagctc gccgtcgcgg tagacgcggt agccgggcag gtcggtggcg
     9481 atgtcggagc cgggcgcggc gcgcaccgcg cgcgcgccgg gcgccagcac ctcgacgatc
     9541 gggcaggcct gcgggttgcg ctggcagaac agcaggaagt cgaacgcgtc ggcctgcggg
     9601 acgatcgtca ggttggcctg cacccgcccc ggcgcgtgac cggccgtcgg ccggtcgaat
     9661 tcgccgctgc ggcacgccag gcgcagcgcg gcggggtcgt cgtggtggat gggcatgggg
     9721 acattctgga aatcgtggcg gcgcggcgca agcgcaaaac ttttctctga gcctatcgat
     9781 ttttcttctg cgccagacgc gccttgccgg cggtgccgcc gaacgcgtcg atgaacttca
     9841 gcgcgtcctg gatcacggag gtcagcgacg acgagccggg gtcgtggcgg tagctcgcca
     9901 gcaccggcag cggcgacagc gtcgtcgcgc agggcaggat cgcgatctcg tgctggcgca
     9961 ccagcgactc ggcggcgatg cgcggcagcg tcgcgatgcc gaagccggcc tcgatcaggt
    10021 gcaccatcgc cgggatcgat gacaccgcat gcaaccgcgg cggctccagg ccctcgccgc
    10081 gcaactgctc gagcagcgcc aggtgcggct gcgagccgcg ctggaaggtc atcacctcgt
    10141 gcgcgacgat gtccgcgagc gtgtacggcg tccggcgctt cagcgacttc gcgccgacga
    10201 acaccatctc catcgtcggc agcggacggg tgcggatgtc gggcgcggac accgattgcg
    10261 cagcgaacac gagatccagc gtgccgcgct tgacggcctc ggccagcacc ggcgtgatct
    10321 cgaccgtcag ctcgagctgc agcttcggat gcacgctgcg caggtgctgc agcagcggga
    10381 tcagccacgt gtgcagcacc gactcgatcg cgccgacgcg cagcaggtgc acgccgccgg
    10441 cctcctcggt gccgagctcg tcgcgcatct ggcggtggat ggccatcagg cgctcggcgt
    10501 agtcgaggaa acgcatgccg gcgttggtca gcgcgaactg ctggcgatcg cgccggtcga
    10561 tcagcagggt gcccagctcc tcttccagcg cggacacgcg gctggagatg gccgactgcg
    10621 tcatgaacag cttctcggcc gcccgcgtca tgctcttgag cgtggcgacc cagtagaagg
    10681 cttcgacgaa tcgctggttc atggcgcccc tgttcgtgct tcgcgacgcg cgccgcggtg
    10741 gccctgccgg ccgactttac ccttgcgcca gctgcttcat ctgcgacttc agcatcttgc
    10801 ggccgtcggc gttcgtcgcc cgccaggcct ggagcgcgag cgccaggaac tgctgctcgc
    10861 cgggcgtcag tcccgcggct tcgcgctctt cgtcgagcca gccggcgggc ttgccgcagt
    10921 gctgctcgat ctggcgcgcg agcttgtcgc cgatcgggcg cgaggacttg atctggctcc
    10981 acatgctggg cgagatctcg acgtgcgcgg cgaacgcctg ctccaggccc ttgggcgcgg
    11041 cgccgccggc caccgcgctc tcggcccagg actggaacag ggc
//
LOCUS       DATCRO010000198        12536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_407109, whole genome shotgun sequence.
ACCESSION   DATCRO010000198 DATCRO010000000
VERSION     DATCRO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407109"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VIN75_05280"
     CDS             <1..941
                     /locus_tag="VIN75_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973610.1"
                     /translation="SIDGGKMLNMLRGVGLPPGLAVFNNKLFMAWKGIEHDQRIWFSS
                     FNGSSWTPQQFVSGVATSCGVSLAVFNGKLYMAWKGMDADERIWWSSYNGTSWAPQQF
                     VSGVATSTGPSLAVFGNKLYMGWKGMLSDQRIWWSSFDGSNWAPQQTIAGVATSVGPQ
                     LAVFQGSLYAIWKGMNNDQGLWYSHFNGSAWVPQKQVPGVGTSEGASIAVFNNKLYAA
                     WKGMIDDQRIWTASFDGTTWTAQQLISGVGTSVGPALAVFGGVLHMTWKGINGDDRVW
                     HTSFNGSTWAPQQQIVGIGTNPFNMHDNTREPALEA"
     gene            complement(1175..1327)
                     /locus_tag="VIN75_05285"
     CDS             complement(1175..1327)
                     /locus_tag="VIN75_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEY8973611.1"
                     /translation="MNIHLSLTPIISLIAGILVLLMPKLLNYIVGFYLIAIGLIGILG
                     IHQIRI"
     gene            complement(1417..2619)
                     /locus_tag="VIN75_05290"
     CDS             complement(1417..2619)
                     /locus_tag="VIN75_05290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8973612.1"
                     /translation="MLAGAIAFAASLAGCATEQSTGQAPGRAHELPGHAAIAAEVARV
                     MQRTHAQGLAVALIDRGQVRDVGAWGLRNARGEPLATDTVMYGASLTKTVFAYTVMQL
                     VDQGRLALDTPIRDDLDRPLPDVPPDPVFPDKYGPYKDLAGDPRWQAITPRMCLTHST
                     GFDNFWFVEPDQKLRIHFEPGTRFSYSGEGLILLQFVIEHGRADKGLGVDVGELAQAD
                     FRRLGMTRTKLTWRQGEDANVADGWNDRGEPRPHDKRSKVRAAGSMNTTIADFATFAA
                     ALVRGDGLSAASRAEMTRPQLHIATAHQFPHFGPDLPSGEQRPDLAAGLGVVVFDGPQ
                     GHGFFKGGHDGQTANTMVCVERGQRCAVILSNDVRAETAFADLVRFLLGETGVPYDWE
                     YGDYAGKS"
     gene            complement(2853..3773)
                     /locus_tag="VIN75_05295"
     CDS             complement(2853..3773)
                     /locus_tag="VIN75_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEY8973613.1"
                     /translation="MSRLVRGLAVLAGILCATTAHAFSMQFCEHPREPSADQRDVMLR
                     FGAIVRDEIAATGSPAALIARSGLDLHRLDVRYSHAAVVLKDNGERPWSVRELYFSCD
                     EHRPRVYDEGLTGFLLGTDDPDTGYVSLVFLPPERAEPLRRTAVDKQHALGVLGASYS
                     ANAYPFSTLHQNCNQWVIELLADAWSPGDAQADAGIDARTRAQAWLRGQGYLPTVFTV
                     SAHPMTWLPAVVPWLSNDDHPSHEIAHNRYNVSMPASIETFVQAKVPGARRMEICHAG
                     RRVVLHRGWDDIAEGCVPGAGDQTIELTAD"
     gene            complement(3770..4234)
                     /locus_tag="VIN75_05300"
     CDS             complement(3770..4234)
                     /locus_tag="VIN75_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973614.1"
                     /translation="MRHLKKSLATLALLASATPLAALAASSAASSLSDSLTTSSGSVS
                     DSLKGSSHSSSPDEKQVKGDYKVTDVAMVEGQPGFVELHLQPVAANTGVEVYVKVPRA
                     AAETGHVATGAVVTALQRPYGVEFATTQPRAAFFLALADDVVRDTKMAPVAL"
     gene            complement(4464..5423)
                     /locus_tag="VIN75_05305"
     CDS             complement(4464..5423)
                     /locus_tag="VIN75_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY8973615.1"
                     /translation="MAAAAITRYLEHLRVERRVSAHTLAAYGDALARLEKLADEAQRP
                     LDKLQSAHIQRFAAQLHGAGLGARSIALTLSGWRGFFRWWSLQSDALGLKNNPVDGVR
                     APRAPRPLPKALAVDEAVALATHEPPVADEASSQEAEALAARDHAIAELLYGCGLRLG
                     ELLGLDLAAGAGSAGWIDVDDGTAHVFGKGSKRRSVPVGGPALEALNAWLLQRPLLAG
                     APDCGAVFVSRLGNRLSPNQLRSRLKAQGDRAGVATPVHPHMLRHSYASHLLQSSGDL
                     RGVQELLGHASIATTQVYTRLDFQHLAKVYDAAHPRAKRRRAG"
     gene            complement(5429..6121)
                     /locus_tag="VIN75_05310"
     CDS             complement(5429..6121)
                     /locus_tag="VIN75_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY8973616.1"
                     /translation="MNSTSGTDGITEDDIAGYLANTPGFFERHAELLATVQLASPHGP
                     RAVSLQERQMEMLRERIKGLERKIMEMIRYGQDNMAISDRLHRWTRSVMLTHDPERLA
                     PVLVHDLQHQFMIPQAAVRVWGVAPELAHLPCAAEVSEDVKIFATSLVAPYCGANSGF
                     EAASWFENAEAVTSLALIPLRPDRQHTAFGLLALGSPDPTRYTPDMGLEFLSQVGDIA
                     GAALTRLLIQQA"
     gene            complement(6118..6990)
                     /gene="dapF"
                     /locus_tag="VIN75_05315"
     CDS             complement(6118..6990)
                     /gene="dapF"
                     /locus_tag="VIN75_05315"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY8973617.1"
                     /translation="MQPSPHRVRFTKMQGAGNDFVVLDATREPLALGAEQMRRLGDRR
                     FGVGADQILIVERPTTPGMDFSYRIFNNTGDEVEHCGNGARCFARYVRDRGMIGRDTI
                     RVQTVNNELELHIQADGRVRVDMNAPRFDHADLPFDSAGLVATRVGDFETWPLDVDEY
                     GSVDVAVLSMGNPHAVMRVADVDRHPVALIGPLVENHARFPRRVNAGFMQVLSRRHVR
                     LRVHERDAGETLACGTGACAAIVAGIRLGWLDTRVDIDMHGGKLTVEWAGGPDDSVFL
                     TGPATTVFEGEIEL"
     gene            complement(7020..7715)
                     /locus_tag="VIN75_05320"
     CDS             complement(7020..7715)
                     /locus_tag="VIN75_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY8973618.1"
                     /translation="MFTELEITGRARTHVIQHDAPRFAAHPDAAAAFLRLRAAAARDG
                     IELRPYSSFRGYDAQARIWEAKFSGRATLYDIDGKPRDCAGMSEDDVIGHILDWSALP
                     GASRHQWGTEIDVVDGAVMPPGYKPQLLPAEVTDGGLFAPLHRWLDAHIHEHGFFRPY
                     ARFQGGMFPEPWHLSYAPVSMRAIDDLTLDVLRRCTEASDIPGKHRVLERLPEILERH
                     VRNIVPPPASCAW"
     gene            complement(7934..8407)
                     /locus_tag="VIN75_05325"
     CDS             complement(7934..8407)
                     /locus_tag="VIN75_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973619.1"
                     /translation="MKFPQRLVAALIALPLVLAATACVVTPAPPPQHHPAYLHALTDL
                     RDARWNLEHRPGSAAVSGQEDIAIVEIDRAINDATTAAMEDGKNVYQHPQEDAKLDWR
                     GRLHHADELLRKARNDVAQGETNGAAVDLRNRVIGHIDAAIAATERAIHIIEQGA"
     gene            complement(8553..9200)
                     /locus_tag="VIN75_05330"
     CDS             complement(8553..9200)
                     /locus_tag="VIN75_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8973620.1"
                     /translation="MAAPRETVENPLRKTPSQTRAMRTVDTLFEAATRVLQSEGEAGF
                     TTNRIAERAGFSIGTLYQYFPSKEAIVVAMVRRQRERVMRELDAMLERAVAGEIGGEE
                     ALRLYLKQVIDAFGRGQKAQRLLARLGWQLDAPALIVAAMDDAAQAIRSALERLGDPA
                     LPVPDDATLYLLTRAVLGAVRSAAVEEHGMLDDPRFEEALFRMVCGVLFRPADAG"
     gene            9328..10212
                     /locus_tag="VIN75_05335"
     CDS             9328..10212
                     /locus_tag="VIN75_05335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY8973621.1"
                     /translation="MTDLLDASVSATAAAPAAAESTRRLPIRRLRVDLSKGFPKLWNG
                     GEAFRTHYLNALSMSFPIGEQFFIDSVRKGVTMLPPEQQAAWADEMRGFTGQEATHRF
                     LHGQFNAELDRQGLRNYWQGWAAWRIKNGENMHPINHVAVTAAYEHFTAVMATGVLTH
                     PHWLEGATPELALLWRWHAVEESEHKTVAFDLYRAMGGSERRRRLWFVYIAVQFFFES
                     SLQTFINLWRAGLWWQPRTWWQAGRYLFGRRGLWAASFFPLLRYLKRGFHPNDLADAD
                     EMAQWLQGQREAYSIVGG"
     gene            complement(10219..10434)
                     /locus_tag="VIN75_05340"
     CDS             complement(10219..10434)
                     /locus_tag="VIN75_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973622.1"
                     /translation="MEYRFDVFGNRVAIRRVGDDWQAFDLGNEGKRRKAGFVVPAFIE
                     VEGLRQYLADLFHESARRSNDQVTPLD"
     gene            complement(10443..11099)
                     /locus_tag="VIN75_05345"
     CDS             complement(10443..11099)
                     /locus_tag="VIN75_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973623.1"
                     /translation="MKRPSPIATLAALAAALAAAPASSQTAASAPQAEWGASLRTPAP
                     ATEPASAASAPTTIVLPPRSAFAAARANGGLATGDYFHDFGELIVRVRSVKWIEEICS
                     ETFPATAEANRHAYEVWHVDNGPFVDEVEGQFLIIAKYWGDESPTARKEGLTVEQLKA
                     KVDANRAGLRQDFLSRGMKSFQTRCEAYPEILLSPQLDLERSQADLVRSVRMGPGQAR
                     "
     gene            complement(11096..11386)
                     /locus_tag="VIN75_05350"
     CDS             complement(11096..11386)
                     /locus_tag="VIN75_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973624.1"
                     /translation="MTRPALPSVVALCAAALLAVAPPAASAADIAAKPAASPDQPRPR
                     TGAYKVTRRGAHLIRARCLEGGHVVVPTHEKATAAQRDRDIHEACKTVDYTR"
     gene            11596..12039
                     /locus_tag="VIN75_05355"
     CDS             11596..12039
                     /locus_tag="VIN75_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973625.1"
                     /translation="MKSQLLLLLALALASIAAVAAPSEAPLPVRADHPFLGAWQITKR
                     DNDCAEIYRIDRSGRTLITSADEVAQSTFTISDQPSARGYYKWIDTLVKDNGKKDCWG
                     QVTKPGKTTTRYVMMNPAKDRFIVCLAEDGRQCFGPFVKLEGGEI"
     gene            12042..12461
                     /locus_tag="VIN75_05360"
     CDS             12042..12461
                     /locus_tag="VIN75_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973626.1"
                     /translation="MGWEDPSPRGRARAPGGNPAVQPGYGDPGVRLSLTIILMRWAVA
                     LLVGGLVYAGAHKGFGGWLGPAHAMTVLAVLLALAAVVAWLVVRLLMRLPESEGIGIP
                     FGPRRGWDDSSGLTFERAAAVDLTADVVSAFIDAATD"
BASE COUNT         1767 a         4207 c         4694 g         1868 t
ORIGIN      
        1 gcagcatcga cggcggcaag atgctcaaca tgctgcgcgg cgtcggcctg ccgcccgggc
       61 tggccgtctt caacaacaag ctgttcatgg cctggaaggg catcgagcac gaccagcgca
      121 tctggttctc cagcttcaac ggcagcagct ggacgccgca gcagttcgtc tcgggcgtgg
      181 ccaccagctg cggcgtgtcg ctggccgtgt tcaacggcaa gctctacatg gcctggaagg
      241 gcatggacgc ggacgagcgg atctggtggt ccagctacaa cggcacgtcg tgggcgccgc
      301 agcagttcgt ctcgggcgtg gccaccagca ccggcccgtc gctggccgtg ttcggcaaca
      361 agctctacat gggctggaag ggcatgctca gcgaccagcg catctggtgg tccagcttcg
      421 acggcagcaa ctgggcgccg cagcagacga tcgccggcgt ggccaccagc gtcggcccgc
      481 agctggccgt gttccagggc agcctctacg cgatctggaa gggcatgaac aacgaccagg
      541 gcctgtggta ctcgcacttc aacggctccg cgtgggtgcc gcagaagcag gtgcccggcg
      601 tcggtacgag cgagggcgcg tccatcgcgg tgttcaacaa caagctctac gccgcgtgga
      661 agggcatgat cgacgaccag cgcatctgga cggccagctt cgacggcacg acctggacgg
      721 cgcagcagct gatctcgggc gtgggcacca gcgtcggccc cgcgctggcg gtgttcggcg
      781 gcgtgctgca catgacgtgg aagggcatca acggcgatga ccgcgtctgg cacacgagct
      841 tcaacggcag cacctgggcg ccgcagcagc agatcgtcgg catcgggacg aacccgttca
      901 acatgcacga caacacacgc gagccagcgc tcgaggcatg acggcggggg ccggcgctgc
      961 acacgcggcg ccggcccgct cgcatgaccg gcgcgccgcg catcctcgga tgcggatcgc
     1021 ggatcgcgga tcgcggatcg gggtccggga tcggggtcag gtcttgaatg ttgcggggtc
     1081 aggtcttgag tgcacgattc aagacctgac cccacaacat tcaagacctg accccattgg
     1141 cggaccccat tggcgacaac tttggcgtcg cttgtcagat ccggatctgg tggatcccga
     1201 ggatgccgat caggccgatc gcgatcaggt agaagcccac gatgtagttc agcagcttgg
     1261 gcatgagcag gacgaggatg ccggcgatca gggagatgat gggcgtgagc gagagatgga
     1321 tgttcaaggg aagcctcggg aatcggtggc gccggcgcgc gggcgccggc aggcgggcat
     1381 ggtagccggg ctcgcggcgc cgggccgcgc aggatgtcac gacttgccgg cgtagtcgcc
     1441 gtattcccag tcgtagggca cgccggtctc acccagcagg aagcgcacga ggtctgcaaa
     1501 ggcggtctcg gcgcgcacgt cgttggacag gatcaccgcg cagcgctggc cgcgctccac
     1561 gcagaccatc gtgttggcgg tctggccgtc gtggccaccc ttgaagaagc cgtgcccctg
     1621 cggaccgtcg aagacgacga cgcccaggcc ggcagccagg tcggggcgct gctcgccgga
     1681 cggcaggtcg gggccgaaat gcgggaactg gtgcgcggtg gcgatgtgca gctgcgggcg
     1741 cgtcatctcg gcccgcgagg ccgcgctcag gccatcgccg cgcacgaggg cggcggcgaa
     1801 ggtcgcgaaa tcggcgatgg tcgtgttcat cgagccggcc gcgcgcacct tgctgcgctt
     1861 gtcgtgcggc cgcggctcgc cgcgatcgtt ccagccgtcg gcgacgttcg catcctcgcc
     1921 ttggcgccag gtgagcttgg tgcgcgtcat gccgaggcgg cggaagtcgg cctgcgcgag
     1981 ttcgccgacg tccacgccca gccccttgtc ggcgcggcca tgttcgatga cgaactgcaa
     2041 caggatcagg ccttcgccgg aatagctgaa gcgcgtgccc ggctcgaagt ggatgcgcag
     2101 cttctggtcc ggctcgacga accagaagtt gtcgaagccc gtggagtgcg tcaggcacat
     2161 gcgcggcgtg atcgcctgcc agcgcgggtc gcccgccaga tccttgtacg gcccgtactt
     2221 gtcggggaag acggggtccg gcgggacgtc cggcagcggg cggtcgaggt cgtccctgat
     2281 cggcgtgtcc agcgcgagcc ggccctggtc gacgagctgc atcaccgtgt aggcgaacac
     2341 ggtcttcgtg agcgaggcgc cgtacatcac ggtgtcggtg gccagcggct cgccgcgggc
     2401 gttgcgcagg ccccaggcgc cgacgtcgcg cacctggcca cggtcgatca acgcgacggc
     2461 caggccctgc gcgtgcgtgc gctgcatgac gcgggcgacc tcggcggcga tggcggcgtg
     2521 gcctggcagc tcgtgcgcgc gcccgggcgc ttgcccggtc gattgctccg tcgcgcagcc
     2581 ggccagcgag gcggcgaagg cgatggcgcc tgcgagcatc gctgcccgtc gatcgcccag
     2641 gcggtcatcc tgcgcgcagt cacaggatcc acgtgggcat gccgggcggc ttgatggccg
     2701 gggaaggtgg atcctgcgac tgcgcgcagg atgacggttg agcggcgcga cgcagagcgg
     2761 agtgatcgag gctttcgaca tggcaaggcg tccttcgcag tccgacctcc agcatagggg
     2821 agcggctgcc gtcgcgttgc gattcaccgg cctcagtcgg ccgtcagctc gatggtctgg
     2881 tcgccggcgc cgggcacgca gccctcggcg atgtcgtccc agccgcggtg cagcaccacg
     2941 cgccggcccg cgtggcagat ctccatgcgg cgcgcgcccg gcaccttggc ctgcacgaag
     3001 gtctcgatcg aggccggcat gctgacgttg tagcggttgt gcgcgatctc gtgcgacggg
     3061 tggtcgtcgt tggacagcca cggcaccacg gccggcagcc aggtcatcgg atgcgcgctg
     3121 accgtgaaca cggtgggcag gtagccctgg ccgcgcagcc acgcctgggc gcgcgtgcgc
     3181 gcgtcgatgc cggcgtccgc ctgcgcgtcg ccgggactcc aggcgtcggc cagcagctcg
     3241 atcacccact ggttgcagtt ctggtgcagc gtgctgaacg gataggcgtt ggcgctgtag
     3301 ctggcgccca gcacgcccag cgcatgctgc ttgtcgaccg ccgtgcggcg cagcggttcg
     3361 gcgcgctcgg gcggcaggaa gacgagcgag acgtagccgg tgtccgggtc gtcggtgccc
     3421 agcaggaagc cggtcaggcc ctcgtcgtac acgcgcggac ggtgctcgtc gcacgagaag
     3481 tacagctcgc gcaccgacca cggacgctcg ccgttgtcct tgagcacgac ggccgcgtgc
     3541 gagtagcgca cgtccaggcg gtgcaggtcg aggccggagc gcgcgatgag cgccgcgggc
     3601 gatccggtcg cggcgatctc gtcacgcacg atggcgccga agcgcagcat gacgtcgcgc
     3661 tggtcggcgc tcggctcgcg cgggtgctcg cagaactgca tcgagaacgc gtgcgccgtc
     3721 gtggcgcaca ggatcccggc caggacggcc aggccgcgga cgaggcgact caaagcgcca
     3781 cgggcgccat cttcgtgtcg cggacgacgt cgtcggcgag cgccaggaag aacgcggcgc
     3841 gcggctgcgt ggtggcgaac tcgacgccgt acgggcgctg cagggcggtg acgacggcgc
     3901 cggtggccac gtggcccgtt tcggcggcgg cgcgcggcac cttcacgtag acctcgacgc
     3961 cggtgttggc ggccacgggc tgcagatgca gttccacgaa gccgggctgt ccttcgacca
     4021 tggcgacgtc ggtcaccttg tagtcgccct tgacctgctt ttcgtcgggc gagctggagt
     4081 ggctggagcc cttgagcgaa tcggacacgc tgccggaaga cgtcgtcagg ctgtcggaga
     4141 gcgacgaggc ggccgagctg gcggccagcg ccgccagcgg ggtggcgctg gcgagcaggg
     4201 cgagggtggc gaggctcttc ttgaggtggc gcatcggggc tcctgggagg gcagttggga
     4261 gattcgcgtg attctgcgcc aacgcggcgg cgcggtgggt gtcgattacc ttgcgtaact
     4321 gacgggatcg ggcagacctc gccagccgta gggcgggtta gccgcaggcg taacccgccg
     4381 ctcagctcgc gtgctgacgg ggcgctcgag gcattcggcg aagaggggtc ggcgcgcctg
     4441 caaacggcgg gttacgcctg cggctaaccc gccctgcgac gcttggcccg cggatgcgcc
     4501 gcgtcgtaga ccttggccag gtgctggaag tccagccgcg tgtagacctg cgtggtggcg
     4561 atgctggcgt ggcccagcag ttcctgcacg ccgcgcaggt cgccgctgga ctgcagcagg
     4621 tgcgaggcgt agctgtggcg cagcatgtgc ggatgcaccg gcgtggcgac gccggcgcgg
     4681 tcgccctggg ccttcaggcg cgagcgcagc tggttgggcg acagccggtt gccgaggcgg
     4741 ctgacgaaca cagcgccgca gtcgggcgcg ccggccagca gcgggcgctg cagcagccag
     4801 gcgttcaagg cctccagcgc cgggccgccg acgggcacgc tgcgccgctt ggagcccttg
     4861 ccgaacacgt gcgcggtgcc gtcgtcgacg tcgatccagc cggccgaccc ggcgccggcc
     4921 gcgagatcga gccccagcag ctcacccagc cgcaggccgc agccgtacag cagctcggcg
     4981 atggcgtggt cgcgcgcggc cagcgcctcg gcttcctgcg agctcgcctc gtcggcgacg
     5041 ggcggctcgt gcgtggcgag cgcgacggcc tcgtcgaccg ccagcgcctt gggcagcgga
     5101 cgcggcgcgc gcggcgcgcg cacgccgtcg accgggttgt tcttcaggcc cagggcgtcg
     5161 gactgcaggc tccaccagcg gaagaacccg cgccagcccg acagcgtcag cgcgatcgag
     5221 cgcgcgccca ggccggcgcc gtgcaactgc gcggcaaagc gctggatgtg cgcgctctgc
     5281 agcttgtcga ggggacgctg cgcctcgtcg gccagtttct cgaggcgggc gagggcgtcg
     5341 ccatacgcgg ccagggtatg cgccgacacc cggcgctcga cgcgcaggtg ctccaggtag
     5401 cgcgtgatgg ccgcggcggc catggcggtc aggcctgctg gatcagcagg cgggtcagcg
     5461 ccgcgcccgc gatgtcgccg acctgggaca ggaattccag gcccatgtcg ggcgtgtagc
     5521 gcgtcgggtc gggcgagccc agcgccagca ggccgaacgc cgtgtgctgg cgatcggggc
     5581 gcagcgggat cagcgcgagc gaggtgacgg cctccgcgtt ctcgaaccag ctcgcggcct
     5641 cgaagccgga gttggcgccg cagtacgggg cgaccaggct cgtcgcgaag atcttgacgt
     5701 cctcgctgac ctcggcggcg cacggcaggt gggcgagctc gggggcgacg ccccacacgc
     5761 gcacggccgc ctgcgggatc atgaactggt gctgcaggtc gtgcaccagc accggcgcga
     5821 ggcgctcggg gtcgtgcgtg agcatcaccg agcgcgtcca gcggtgcagg cggtccgaga
     5881 tggccatgtt gtcctggccg tagcggatca tctccatgat cttgcgttcc aggcccttga
     5941 tgcgctcgcg cagcatctcc atctggcgct cctgcagcga gacggcgcgc gggccgtgcg
     6001 ggctggccag ctgcacggtg gcgagcagct cggcgtggcg ctcgaagaag ccgggcgtgt
     6061 tggcgaggta gcccgcgatg tcgtcctcgg tgatgccgtc ggtgccgctg gtgctgttca
     6121 cagttcgatc tctccttcga agacggtggt ggccgggccg gtcaggaaca cgctgtcgtc
     6181 cgggccgccg gcccattcga cggtgagctt gccgccgtgc atgtcgatgt cgacgcgggt
     6241 gtcgagccag cccaggcgga tgcccgcgac gatcgccgcg caggcgccgg tgccgcaggc
     6301 gagcgtctcg ccggcgtcgc gctcgtgcac gcgcaggcgc acgtgcctgc gcgagagcac
     6361 ctgcatgaag ccggcgttga cgcgacgagg gaagcgcgcg tggttctcga ccagcgggcc
     6421 gatcagcgcg acggggtggc ggtcgacgtc ggccacgcgc atcaccgcgt gcgggttgcc
     6481 catcgacagc acggccacgt cgacgctgcc gtactcgtcg acatcgagcg gccaggtctc
     6541 gaagtcgccc acgcgcgtgg ccacgaggcc ggccgagtcg aacggcaggt cggcgtgatc
     6601 gaagcgcggc gcgttcatgt cgacgcgcac gcggccgtcg gcctggatgt gcagctcgag
     6661 ctcgttgttg acggtctgga cgcggatggt gtcgcggccg atcatgccgc ggtcgcgcac
     6721 gtagcgggcg aagcagcgcg cgccgttgcc acagtgctcc acctcgtcgc cggtgttgtt
     6781 gaagatgcgg tagctgaagt ccatgcccgg cgtggtcggg cgctcgacga tcaggatctg
     6841 gtcggcgccg acgccgaacc ggcggtcgcc cagccggcgc atctgctcgg ccccgagcgc
     6901 cagcggctcg cgcgtggcgt cgagcacgac gaagtcgttg ccggcgcctt gcatcttggt
     6961 gaagcgaacg cggtgcgggg agggctgcat ggggcgatta tcgccgcagg gggatgccct
     7021 caccaggcgc agcttgccgg gggagggacg atgttgcgca cgtgccgctc caggatctcg
     7081 ggcagccgtt cgagcacgcg gtgcttgccg gggatgtccg aggcctcggt gcagcgccgc
     7141 agcacgtcga gcgtgaggtc gtcgatggcg cgcatggaca ccggcgcgta gctgaggtgc
     7201 cagggctcgg ggaacatgcc gccctgaaag cgcgcgtagg ggcgaaagaa gccgtgttcg
     7261 tggatgtggg cgtcgagcca gcggtgcagc ggcgcgaaca ggccgccgtc cgtgacctcg
     7321 gccggcagca gctgcggctt gtagccgggc ggcatgacgg cgccgtcgac gacgtcgatc
     7381 tccgtgcccc actggtggcg gctggcgccg ggcagggcgg accagtcgag gatgtggccg
     7441 atgacgtcgt cctcgctcat gccggcgcaa tcgcgcggct tgccgtcgat gtcgtacagc
     7501 gtcgcgcggc cgctgaactt cgcctcccag atgcgcgcct gggcgtcgta gccgcggaac
     7561 gagctgtagg gccgcagctc gatgccgtcg cgcgcggccg cggcgcgcag gcgcaggaac
     7621 gcggcggcgg cgtcggggtg ggccgcgaag cgcggggcgt cgtgctggat gacgtgcgtg
     7681 cgggcgcggc cggtgatctc gagctcggtg aacatgtccg catcatgccg tgggcgcatc
     7741 aagccctcgg gcggcgcgcc ccctcggacg cggcctagcg ccaccacgcc gtcatcctgc
     7801 gcgcagtcgc aggatccacg gcggcgtgcc gggcggcctc acggctgggc caggtggatc
     7861 ctgcgactgc gcgcaggatg acacctgttc atgcaggccc gctgcgccgc gcggcgcggg
     7921 cccgaccggt cgttcaggcg ccctgctcga tgatgtggat cgcgcgctcg gtcgccgcga
     7981 tcgcggcgtc gatgtggccg atcacgcgat tgcgcaggtc gacggcggcg ccgttggtct
     8041 cgccctgggc gacgtcgttg cgcgccttgc gcagcagttc gtcggcgtgg tgcaggcggc
     8101 cgcgccagtc cagcttggca tcttcctgcg ggtgctggta gacgttcttg ccgtcttcca
     8161 tcgcggccgt cgtggcgtcg ttgatggcgc ggtcgatctc gacgatcgcg atgtcctcct
     8221 ggccgctgac cgcggcgctg cccgggcggt gctccaggtt ccagcgcgcg tcgcgcaggt
     8281 cggtcagcgc gtgcaggtag gccgggtggt gttgcggcgg cggcgccggc gtgacgacgc
     8341 aggcggtggc ggccagcacg aggggcagtg cgatcagcgc ggcgacgagg cgttgcggga
     8401 acttcatggt ctctctctcc gttcgtggct tgtgtgtgac ggggtgggcc gggttgtagc
     8461 ccgtgaagct gagcccggcc tgttccgtcg aacgcggaga cctgagccga cgccgacagg
     8521 aaccggttcc aggccggccc gaggaaaccc cgtcaaccgg cgtctgccgg ccggaacagc
     8581 acgccgcaca ccatgcggaa cagcgcttcc tcgaagcgtg gatcgtccag catcccgtgc
     8641 tcctcgacgg ccgcggagcg caccgcgccc agcactgcgc gcgtgagcag gtaaagcgtc
     8701 gcgtcgtcgg gcacgggcag cgccgggtcg ccgaggcgct ccagcgcgga gcggatcgcc
     8761 tgcgcggcgt cgtccatcgc cgccacgatc agtgccggcg cgtcgagctg ccatcccagg
     8821 cgcgcgagca ggcgctgggc cttctggccg cggccgaagg cgtcgatcac ctgcttcagg
     8881 tacaggcgca gggcctcctc gccgccgatc tcgccggcca ccgcgcgctc cagcatcgca
     8941 tcgagctcgc gcatcacgcg ctcgcgctgg cgccggacca tcgccacgac gatggcctcc
     9001 ttgctgggga agtactggta gagcgtgccg atcgagaaac cggcgcgctc ggcgatgcga
     9061 ttggtggtga acccggcctc gccctcggac tgcaaaaccc gagtcgcggc ttcgaacagc
     9121 gtatcgacgg tgcgcatcgc gcgcgtctgc gacggcgtct tgcgcagggg gttttcgacg
     9181 gtttcgcggg gagcggccat ggccgcactc taacggttcc gcgccgtcgg cggccgggca
     9241 acccccgggt cgccaaaagc gagtagaccg cggccgcggc gccacttacg ctttccccca
     9301 ggaaaccaac ccgacttcgc caacgccatg accgacctgc tcgacgcttc cgtgagcgca
     9361 actgccgcag cccccgccgc ggccgaatcg acgcgccgcc tgccgatccg ccgcctgcgc
     9421 gtcgacctgt ccaagggctt cccgaagctg tggaacggcg gcgaggcctt ccgcacgcac
     9481 tacctgaacg cgctgtcgat gagcttcccg atcggcgagc agttcttcat cgactccgtg
     9541 aggaagggcg tgacgatgct gccgcccgag cagcaggccg cctgggccga cgagatgcgc
     9601 ggtttcacgg gccaggaggc cacgcaccgc ttcctgcatg ggcagttcaa cgccgagctc
     9661 gaccgccagg gtttgcgcaa ctactggcag ggctgggccg catggcgcat caagaacggc
     9721 gagaacatgc atccgatcaa ccacgtggcc gtcaccgccg cgtacgagca cttcaccgcg
     9781 gtcatggcca cgggcgtgct gacgcatccg cactggctgg agggcgccac gcccgagctc
     9841 gcgctgctgt ggcgctggca cgcggtcgag gaaagcgagc acaagacggt cgccttcgac
     9901 ctgtaccgcg cgatgggcgg cagcgagcgg cggcgtcggc tgtggttcgt ctacatcgcg
     9961 gtgcagttct tcttcgaatc gagcctgcag accttcatca acctgtggcg cgcgggcctg
    10021 tggtggcagc cgcgcacgtg gtggcaggcc ggccgctacc tgttcggacg gcgcggcctg
    10081 tgggccgcca gcttcttccc gctgctgcgt tacctgaagc gcggcttcca cccgaacgac
    10141 ctcgccgacg cggacgagat ggcgcagtgg ctgcagggac agcgcgaggc gtattcgatc
    10201 gtcgggggct gaggcgcgtc agtccagcgg cgtgacctgg tcgttggagc gcctggccga
    10261 ctcgtggaac aggtcggcga ggtactggcg caggccctcg acctcgatga acgcgggcac
    10321 gacgaagccc gccttgcggc gcttgccttc gttgccgagg tcgaacgcct gccagtcgtc
    10381 gccgacgcgg cggatcgcca cgcggttgcc gaagacgtcg aagcggtact ccacggccgg
    10441 cctcagcgcg cctgccccgg ccccatgcgc accgaacgca cgaggtcggc ctgcgagcgc
    10501 tccaggtcca gctgcggcga cagcaggatc tccggatacg cctcgcagcg ggtctggaag
    10561 gacttcatgc cgcgcgacag gaagtcctgc cgcagcccgg cgcggttggc gtcgaccttg
    10621 gccttgagct gctccacggt cagaccttcc ttcctggcgg tgggcgactc gtcgccccag
    10681 tacttggcga tgatcaggaa ctgcccctcg acctcgtcga cgaacgggcc gttgtccacg
    10741 tgccagacct cgtacgcgtg gcggttggcc tcggcggtgg cggggaaggt ctcgctgcag
    10801 atctcctcga tccacttgac gctgcgcacg cgcacgatca gctcgccgaa gtcgtggaag
    10861 tagtcgcccg tggccaggcc gccgttggcg cgcgccgcgg cgaacgcgct gcgcggcggc
    10921 agcacgatgg tggtgggcgc gctggcggcg gacgcgggct cggtggccgg cgccggcgtg
    10981 cgcagcgacg cgccccattc ggcctgcggc gcggaggcgg cggtctgcga ggacgcgggg
    11041 gcggccgcga gggccgcggc cagcgcggcg agggtggcga tcggcgatgg gcgcttcatc
    11101 gggtgtagtc gacagtcttg caggcttcgt ggatgtcgcg gtcgcgctgg gcggccgtgg
    11161 ccttctcgtg cgtgggcacg acgacgtggc cgccctcgag gcagcgggcg cggatcaggt
    11221 gcgcgccgcg cctggtgacc ttgtaggcgc cggtgcgcgg cctgggctgg tccgggctgg
    11281 cggccggctt ggctgcgatg tccgcggcgg acgcggccgg cggcgcgacg gcgagcaggg
    11341 ccgcggcgca cagagcgacg acggacggga gggcggggcg ggtcatggca gtctcctgtg
    11401 tcctgaacgg cggctgaccg ccggcgcggc aagcggggcg ccggccgttc ccggacatgc
    11461 ggacgagcgg cgattcttcc atacgcatgt gtcgcgtcct tgtccgcgat ggcccgagca
    11521 ggcccgcccg cgctcaggca gaatcgatcg aaacggagcc gctggcgtcg gcgcctccaa
    11581 ggacaaggcc tccgcatgaa atcccagctg ctgctgctgc tcgccctggc gctcgcctcg
    11641 atcgccgccg tcgccgcgcc gtccgaggcg ccgctgcccg tgcgcgccga ccacccgttc
    11701 ctgggcgcct ggcagatcac caagcgcgac aacgactgcg ccgagatcta ccgcatcgac
    11761 cgcagcggac gcaccctgat caccagcgcc gacgaggtcg cgcagtcgac gttcacgatc
    11821 tccgaccagc cgagcgcgcg cggctactac aagtggatcg atacgctggt caaggacaac
    11881 ggcaagaagg actgctgggg ccaggtcacc aagcccggca agaccaccac gcgctacgtg
    11941 atgatgaacc cggccaagga ccgcttcatc gtctgcctgg ccgaggacgg gcgccagtgc
    12001 ttcggaccgt tcgtcaagct cgagggcggg gagatctgac gatgggctgg gaagacccgt
    12061 cgccgcgcgg ccgtgcgcgc gcgcctgggg gcaacccggc ggtgcaaccg ggctacggcg
    12121 atccgggcgt gcggttgtcg ctcaccatca tcctgatgcg gtgggcagtc gcgctgcttg
    12181 tcggtggcct cgtgtacgcc ggcgcgcaca agggattcgg cgggtggctc gggccagcgc
    12241 acgcgatgac ggtgctcgcg gtcttgctgg ccctggccgc ggtcgttgcc tggctcgtgg
    12301 tgcgcctgtt gatgcgcctg cccgagtccg agggcatcgg gatcccgttc ggccctcgcc
    12361 gcggctggga cgacagctcc ggcctgacat tcgaaagagc cgcggccgtc gacctgaccg
    12421 ccgacgtcgt cagtgcgttc atcgacgcgg caacggatta gcgccggatc gggtcaatac
    12481 aggctcgcct cgcccggcgg ccgcgtcttg aagcgcttgt gcacccagta gtactg
//
LOCUS       DATCRO010000199         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_902086, whole genome shotgun sequence.
ACCESSION   DATCRO010000199 DATCRO010000000
VERSION     DATCRO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902086"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..826)
                     /gene="efp"
                     /locus_tag="VIN75_05365"
     CDS             complement(275..826)
                     /gene="efp"
                     /locus_tag="VIN75_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8973627.1"
                     /translation="MKIAQEIRAGNVIMQGKDPMVVLKTEYSRGGRNAATVRMKMKNL
                     LNGSGAEVVFKADDKMDQIILDKKECTYTYFADPMYVFMDADYNQFEVEAENMGDAIN
                     YLEDNMPVEVVFYDGKAISVELPTTVVREVTTEPAVKGDTSGKVMKPAKIATGFEIAV
                     PLFVESGDKIEIDTREHVYRKRV"
     gene            complement(978..2105)
                     /gene="earP"
                     /locus_tag="VIN75_05370"
     CDS             complement(978..2105)
                     /gene="earP"
                     /locus_tag="VIN75_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEY8973628.1"
                     /translation="MTPTASNDATPSLLWDVFCRVIDNFGDIGVCWRLACNLAERGQR
                     VRLWVDDPSALRWMAPDGHDGVDVAAWIASTGFPPPGDVVVEAFGCDPAPAFLAAMAA
                     RAEQARGPVWINLEYLSAEDYVERSHGLASPQMSGPARGLVKWFFYPGFTARTGGLLH
                     EAALESEQAVFDRAGWLASQGLSLAPDERLVSVFCYPGAPLDRLVASLAADGRPTTIA
                     TAPGAATGAARAALAMAGEAAAALRQVALPWLTQAGYDRLLWASDLNFVRGEDSWVRA
                     HWAGRPFIWQAYPQDDGAHGAKIAAFLERSLARAAPDAAEAIRDWTAAWNALDDPAAP
                     LTAWTPQALDAAADAARAWRAQLRASPDLAARLLAFVAEKR"
BASE COUNT          365 a          792 c          851 g          380 t
ORIGIN      
        1 tcaggtcttg aatgttgcac ggcgacacct cgccttgggg tcaggtcttg aaagttgcgc
       61 gttgaggcgt cagcgctcgc cgaagccttg ccgcgcaact ttcaagacct gaccccggtg
      121 ccatgccgtt gaacgcgcaa cattcaagac ctgaccctgg cgcggtgctc aacgcgcgaa
      181 tttcaagacc tgacaccgtc tggacgatgc cgcgcaaaga aaaagcgcct gcggcaactg
      241 cctgcaggcg ctcttcgcgc ggagccgggg aggctcagac gcgcttgcgg tacacgtgct
      301 cgcgcgtgtc gatttcgatc ttgtcgccgg attcgacgaa cagcgggacg gcgatctcga
      361 agccggtggc gatcttggcg ggcttcatga ccttgcccga ggtgtcgccc ttgacggccg
      421 gctcggtggt gacttcacgc acgaccgtgg tgggcagttc gaccgagatg gccttgccgt
      481 cgtagaacac cacttcgacc ggcatgttgt cctcgaggta gttgatggcg tcgcccatgt
      541 tctcggcttc cacctcgaac tggttgtagt cggcgtccat gaacacgtac atcgggtcgg
      601 cgaagtacgt gtacgtgcat tccttcttgt cgaggatgat ctggtccatc ttgtcgtcgg
      661 ccttgaagac gacttcggca cccgaaccgt tgagcaggtt cttcatcttc atgcgcacgg
      721 tggcggcgtt gcggccaccg cggctgtatt cggtcttgag cacgaccatc gggtccttgc
      781 cctgcatgat gacgttgccg gcgcggattt cctgagcgat tttcatgggt cgattccaga
      841 aactagtcga gactagggag cgcgttactg tcgggtcgag gatggggcgc cggacgcggg
      901 caccgggggt gcggccgcgt ggcgttcgct cgcgcagccg gacttcctgc cgctgacgta
      961 acgcaaagcc ctttattcta ccgcttttcg gccacgaagg cgaggaggcg ggcggccagg
     1021 tccggcgacg cccgcaattg cgcgcgccag gcccgcgcgg cgtcggcggc ggcgtccagc
     1081 gcctgcggtg tccaggcggt cagcggggcg gcggggtcgt ccagggcgtt ccaggcggcc
     1141 gtccagtccc ggatcgcctc ggcagcgtcg ggcgccgcgc gcgccagcga gcgctcgagg
     1201 aaggccgcga tcttggcacc gtgggcaccg tcgtcctgcg ggtaggcctg ccagatgaac
     1261 ggccgtccgg cccagtgggc gcgcacccag gagtcctcgc cgcggacgaa gttcaggtcg
     1321 gacgcccaca gcaggcggtc gtagccggcc tgcgtgagcc agggcagggc gacctggcgc
     1381 agcgcggcgg ccgcctcgcc ggccatcgcg agcgcggcgc gcgcggcgcc ggtggccgcg
     1441 cccggcgcgg tggcgatcgt cgtcggccgg ccatcggcgg ccagcgatgc gacgaggcga
     1501 tcgagcggcg cgccggggta gcagaacacg ctgacgaggc gctcgtccgg cgccagcgac
     1561 aggccctgcg acgccagcca gccggcgcgg tcgaacaccg cctgctcgga ctcgagcgcg
     1621 gcttcgtgca gcaggccgcc ggtgcgcgcg gtgaagccgg ggtagaagaa ccatttgacg
     1681 aggccgcgcg ccggcccgct catctgcggc gaggccagcc cgtggctgcg ctcgacgtag
     1741 tcctcggcgc tgaggtattc caggttgatc cagaccggcc cgcgggcctg ttccgcgcgg
     1801 gcggccatgg cggccaggaa ggcgggcgcg gggtcgcagc cgaaggcctc gacgacgacg
     1861 tcgccgggcg gcgggaaccc ggtcgacgcg atccaggccg cgacgtcgac gccgtcatgc
     1921 ccgtccggcg ccatccagcg cagcgcgctc ggatcgtcca cccacagccg cacccgctgc
     1981 ccgcgctcgg ccaggttgca ggcgaggcgc cagcacacgc cgatgtcgcc gaagttgtcg
     2041 atgacgcggc agaacacgtc ccacagcagg gacggcgtcg cgtcgttgga ggcggtcggg
     2101 gtcatcgcga cgattatccg cagcggtggc acccgcatct cgggtgtcag gtcttgaatg
     2161 ttgcggtgtc aggtcttgaa tgttgcggac gaccgtcgaa tgacaaggag cgacgtccgg
     2221 aggcggcccg cgcaacattc aagacctgac accgcaacat tcaagacctg acaccgggcg
     2281 ggtggcgccc gcggcacaat gaggagtcca tgcctcgatc cccatcctcg aaatccccgc
     2341 ccgccggtgg caggacgccg gccgcggtcg ccaagaaggg cggcccga
//
LOCUS       DATCRO010000200         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_812453, whole genome shotgun sequence.
ACCESSION   DATCRO010000200 DATCRO010000000
VERSION     DATCRO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812453"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VIN75_05375"
     CDS             complement(<1..619)
                     /locus_tag="VIN75_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8973629.1"
                     /translation="MTPSPTLARAALAAAALALLAGCAASRIDDNVAAAHAEVAAHGG
                     PDFAWLATDAARQQARSDVARALAQPLSEDDAVRIALSHEPALQALLFDRAAASADAT
                     QSARLPNPVFALERLAGGGAAGPVEVTRALTVPLLDLLLLPARAAQEGDTQRRLRLQL
                     AADVLRAANGARTAWVAAVAAEQSLRYANDVGDAADAAADLARRLE"
     gene            complement(702..1085)
                     /locus_tag="VIN75_05380"
     CDS             complement(702..1085)
                     /locus_tag="VIN75_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973630.1"
                     /translation="MRRLLRLFTVLLIALALPIHGAQAATAMAGHASTPSHERMVMPD
                     GTTMDAADMPGASDAKAGACHGHDLGQGGCCSDCCGPIAAPHEMLDVVPVAARWATLP
                     RDAARAAAPQFLTGGTDRPPRAVLA"
     gene            complement(1130..2002)
                     /locus_tag="VIN75_05385"
     CDS             complement(1130..2002)
                     /locus_tag="VIN75_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8973631.1"
                     /translation="MKLLRHGPRGHERPGLVDAGGVLRDLSAQIGDIGAADLAPARLD
                     RLRSVEIDALPVIPRDTRLSVPWHGMSKFVCIGLNYKDHAAESKMALPAEPVLFLKAS
                     SALTGPNDPVVLPQGSAKTDWEVELGVVIGTRARCVSEARALSHVAGYCVVNDVSERA
                     FQLERGGTWDKGKGCDTFGPVGPWLVTADEVPDPQKLRLWLDVGGERMQDGTTADMVF
                     GVAQLVSYVSRFMTLEPGDLIATGTPAGVGMGRGRFLKAGDTLRLGITGLGEQSTTVH
                     AWDAALIDRQHGAH"
     gene            complement(2067..2693)
                     /locus_tag="VIN75_05390"
     CDS             complement(2067..2693)
                     /locus_tag="VIN75_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY8973632.1"
                     /translation="MDLDPLEMVAEPSGFGDDDTTSAAQHRKHARLNAAVAITVALLA
                     TFMGICKIKDDNVCQAMQQAQADKIDHWSYYQARNVRQEVAEATATQLRLQQASQPAP
                     AQAAYAEAIARYDQLAKDQALKKQQVAAQADADQKNYDALNYRDDQFDLSDALLAIAI
                     SMLAVSSLTQLLWLYLLSLFPAACGVLMGVAGLTGLHLHPDKLTALLS"
     gene            2921..>5075
                     /locus_tag="VIN75_05395"
     CDS             2921..>5075
                     /locus_tag="VIN75_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8973633.1"
                     /translation="MHLPQTSLVNRLTVTGLVAVLASAGVLGTVATLLARQSLATQAQ
                     EKLTVVGDAASLRIGEWVAGNLRTSASVTAAAAPDAIGKAMGEPTHAGFARKRRGAPA
                     AHAAHAPQDLIALAMKEPTRAGNDAKPDAAAAAAMAAMAALGKPAAAPAPAPAPAPAK
                     VEAKAAPSPAPAPAPASGGAAAAAAAAMAAVNKAPAAAPAKAEPAKAAATPAPAPAPA
                     AAVAPAPSDAASAPAPAADDQALRIALGQAVSSGDFRTVYMAYDADKRFVQVPPQKLP
                     EGYDPTGRPWYKAALAAGKPIVTEPYKDASFNDMTVTFALPVKADGKLVGVIGADKSL
                     AEVVRMVGGIKPSENSYAFVLDKQGKVLIHPNTDLALKPLAEAQPGMASVASAPDNAI
                     VDANDGTRDVQVRSEAIPGTDWRLAVAYDKSAAMAGLGTLVSSIAVAALAVVAVAGLI
                     LTSMLRRWFEPLRKVRSAMVAVAGGGGDLTARIAVTHNDEVGQIARAFNDFTDSLRGI
                     IHEVKQASSEVKTAAHEIATGNADLSARTESQASAIQQSASSIAQLHESTVTNADSAR
                     EANRIAQEAATLAAEGGDKVHEVVETMGGITASSKRIGEIIGVIDSIAFQTNILALNA
                     AVEAARAGEQGRGFAVVASEVRTLAQRSGEAARDIRALIQKSTQDVEAGAARVDDAGR
                     TISQVVESVRSVSSLMARIVEATQQQTGGIGEVNNA"
BASE COUNT          707 a         1876 c         1854 g          638 t
ORIGIN      
        1 cctccaggcg ccgcgccagg tcggcggcgg cgtccgcggc atcgccgacg tcgttcgcgt
       61 agcgcagcga ctgctccgcc gcgacggccg ccacccacgc cgtccgtgcg ccgttggccg
      121 cgcgcagcac gtcggccgcc agttgcagcc gcaggcggcg ctgcgtgtcg ccctcctggg
      181 cggcgcgggc gggcagcagc agcaggtcca gcagcggcac ggtcagcgcg cgcgtcacct
      241 cgaccgggcc ggccgcgccg ccgccggcca ggcgttccag cgcgaacacc gggttgggca
      301 gccgtgccga ctgcgtggcg tcggccgatg ccgcggcgcg gtcgaacagc agcgcctgca
      361 gcgcgggctc gtgcgacagc gcgatgcgca cggcatcgtc ctcggacagc ggctgcgcca
      421 gcgcgcgggc gacgtccgag cgggcctgct ggcgcgcggc gtcggtggcg agccacgcga
      481 agtcggggcc gccgtgcgcc gcgacttcgg catgggcggc ggcgacgttg tcgtcgatcc
      541 gcgaagcggc gcagccggcc agcaacgcga gcgccgcggc cgccagcgcc gcgcgggcga
      601 gcgttggtga tggggtcatg gaatcctcgt cctggatgca gtcccgcgcg cacgaaggcg
      661 cgggtgctcg cgtcacgcgc ggaggcgcgc gacgtgtcgg ttcaggcgag gacggctcga
      721 ggcgggcggt cggtgccgcc ggtcaggaat tgcggcgctg cggcccgcgc ggcgtcgcga
      781 ggcagcgtgg cccagcgcgc ggcgacaggc acgacgtcca gcatctcgtg cggcgcggcg
      841 atcgggccgc agcagtcgct gcagcagccg ccctggccga ggtcgtggcc gtggcaggcg
      901 ccggccttgg cgtcggacgc gccgggcatg tcggccgcgt ccatcgtcgt gccgtcgggc
      961 atcaccatgc gctcgtgcga cggcgtgctc gcgtggccgg ccatggcggt cgccgcctgc
     1021 gcgccgtgga tcggcagcgc gagcgcgatg agcaagaccg tgaaaaggcg cagcaggcga
     1081 cgcatggcgg cagtctatca acgcggcccg cgccgcgccg cgcgtgcggt caatgggcgc
     1141 cgtgctggcg atcgatcagg gccgcgtccc acgcgtgcac cgtggtcgac tgctcgccca
     1201 ggccggtgat gcccaggcgc agcgtgtcgc ccgccttcag gaatcggccg cggcccatgc
     1261 cgacgccagc cggggtgccg gtggcgatga ggtcgcccgg ctcgagcgtc atgaagcggc
     1321 tgacgtagct caccagctgg gccacgccga acaccatgtc ggccgtcgtg ccgtcctgca
     1381 tgcgctcgcc gccgacgtcc agccacagcc tgagcttctg cgggtcgggc acctcgtcgg
     1441 ccgtcaccag ccacgggccg accgggccga aggtgtcgca acccttgccc ttgtcccagg
     1501 tgccgccgcg ctcgagctgg aacgcgcgct cggagacgtc gttgacgacg cagtagcccg
     1561 ccacgtgcga cagcgcccgg gcctcggaca cgcagcgcgc gcgcgtgccg atcaccacgc
     1621 ccagctcgac ctcccagtcg gtcttggccg agccttgcgg cagcaccacc gggtcgttcg
     1681 ggccggtcag cgcgctggac gccttcagga acaggacggg ctcggcgggc agcgccatct
     1741 tcgattcggc cgcgtgatcc ttgtagttca ggccgatgca gacgaacttg gacatgccat
     1801 gccacggcac cgacagccgc gtgtcgcgcg ggatcacggg cagcgcgtcg atctccaccg
     1861 agcgcagacg gtccaggcgc gcgggcgcga ggtcggcggc gccgatgtcg ccgatctgcg
     1921 cgctcaggtc gcgcaggacg cccccggcgt cgacgaggcc gggccgttca tggccgcgcg
     1981 ggccgtggcg gagcagtttc atgcggatcc tttcgaactg cgcacgatga tgccacccgt
     2041 ggcacgggct cgcagttggc gaggtcttag ctcagcaggg ccgtcagctt gtccgggtgg
     2101 aggtgcagcc cggtgagccc cgccacgccc atgaggactc cgcacgccgc cgggaacagg
     2161 ctcagcaggt acagccagag caactgcgtc agcgacgaca ccgccagcat ggagatggcg
     2221 atggccagca gcgcgtcgga caggtcgaac tggtcgtcgc ggtagttgag cgcgtcgtag
     2281 ttcttctggt cggcgtcggc ctgcgcggcg acctgctgct tcttgagcgc ctggtccttg
     2341 gcgagctggt cgtacctggc gatcgcctcg gcgtacgccg cctgcgccgg cgccggctgc
     2401 gacgcctgct gcagccgcag ctgcgtggcg gtggcctcgg ccacctcctg gcgcacgttg
     2461 cgcgcctggt agtacgacca gtggtcgatc ttgtcggcct gcgcctgctg catggcctgg
     2521 cagacgttgt cgtccttgat cttgcagatg cccatgaagg tggccagcag cgccacggtg
     2581 atcgcgacgg cggcgttcag gcgcgcgtgc ttgcggtgct gggcggccga agtggtgtcg
     2641 tcgtcgccga agccggaggg ctcggcgacc atttcgaggg gatcgagatc catggcggtg
     2701 cgtggggcgg tacagggacg aaccggcgat ttgacaccgc cagcctgaac aagtggacag
     2761 gttcgcgcca accgcgcgat tccggcccgg attggtcctg ccgagcgcgg aaccgcggga
     2821 aatcgggctc ttttccgcgc ttggccggcg cgcgaaccca tcaagatagc ccgactggac
     2881 agccgtccca gccaccgcca ttgccagccg aaagatcgcc atgcacctgc cgcagaccag
     2941 cctcgtcaac cgcctcaccg tcaccgggct cgtcgccgtg ctcgccagtg ccggcgtgct
     3001 gggcacggtg gccacgcttc tggcgcgcca gtcgctggcc acgcaggcgc aggagaagct
     3061 gacggtcgtc ggcgacgccg cgtcgctgcg catcggcgag tgggtggccg gcaacctgcg
     3121 caccagcgcc agcgtcaccg cggccgccgc gccggacgcg atcggcaagg cgatgggcga
     3181 gcccacgcac gccgggtttg ccaggaagcg ccgcggcgcg cctgcggccc atgccgcgca
     3241 tgcgccgcag gatctcatcg cgctggccat gaaggagccc acgcgcgccg gcaacgacgc
     3301 caagccggac gcggctgccg ccgcggcgat ggccgcgatg gccgcgctcg gcaagcctgc
     3361 cgcagcgccc gctcccgcgc ccgcgcccgc accggcgaag gtcgaggcca aggccgcccc
     3421 gtctccggct ccggcccccg cgccggcctc cggcggagcg gccgccgcgg ccgccgccgc
     3481 gatggccgcc gtcaacaagg cgccggccgc cgcgcccgcg aaggccgagc cggccaaggc
     3541 cgctgcgacg cccgcgcccg ctcccgcacc ggccgccgcg gtggcgcccg cgccgtccga
     3601 cgccgcctcc gcgccggccc cggcggccga cgaccaggcc ctgcgcatcg cgctcggcca
     3661 ggccgtctcc agcggcgact tccgcaccgt ctacatggcc tacgacgccg acaagcgctt
     3721 cgtccaggtg ccgccgcaga agctgcccga aggctacgac ccgacgggcc gcccctggta
     3781 caaggccgcg ctggccgccg gcaagcccat cgtcaccgag ccctacaagg acgcgtcgtt
     3841 caacgacatg accgtcacct tcgcgctgcc ggtcaaggcc gacggcaagc tggtgggcgt
     3901 gatcggcgcc gacaagtcgc tggccgaggt cgtcaggatg gtgggcggca tcaagcccag
     3961 cgagaactcg tacgccttcg tgctggacaa gcagggcaag gtcctgatcc acccgaacac
     4021 cgacctcgcg ctcaagccgc tggccgaggc gcagccgggc atggcctcgg tggcctcggc
     4081 gccggacaac gcgatcgtgg acgccaacga cggcacgcgc gacgtccagg tgcgctcgga
     4141 agccatcccc ggcaccgact ggcgcctggc cgtggcctac gacaagtcgg ccgcgatggc
     4201 gggcctgggc acgctggtca gctcgatcgc cgtggccgcg ctcgcggtgg tcgcggtcgc
     4261 gggcctgatc ctcacgagca tgctgcgccg ctggttcgag ccgctgcgca aggtgcgcag
     4321 cgcgatggtg gccgtggccg gcggcggggg cgacctcacc gcgcgcatcg ccgtcaccca
     4381 caacgacgag gtcggccaga tcgcccgcgc gttcaacgac ttcaccgatt cgctgcgcgg
     4441 catcatccac gaggtcaagc aggccagctc cgaggtcaag accgcggcgc acgagatcgc
     4501 caccggcaac gccgacctgt cggcccgcac cgagagccag gccagcgcca tccagcaatc
     4561 ggccagctcg atcgcgcagc tgcacgagtc cacggtgacc aacgccgact cggcgcgcga
     4621 ggccaaccgc atcgcgcagg aagccgcgac gctggcggcc gagggcggcg acaaggtgca
     4681 cgaggtcgtc gagaccatgg gcggcatcac cgcctccagc aagcgcatcg gcgagatcat
     4741 cggcgtgatc gactcgatcg cgttccagac caacatcctc gcgttgaacg cggccgtcga
     4801 ggcggcgcgc gccggcgagc agggccgcgg cttcgccgtc gtggcctccg aggtgcgcac
     4861 gctggcccag cgcagcggcg aggccgcgcg cgacatccgc gcgctgatcc agaagtccac
     4921 ccaggacgtc gaggccggcg ccgcgcgcgt cgacgacgcc ggccgcacga tctcgcaggt
     4981 cgtcgagtcg gtgcgctcgg tgagctcgct gatggcccgc atcgtcgagg ccacccagca
     5041 gcagacgggc ggcatcggcg aggtcaacaa cgccg
//
LOCUS       DATCRO010000201         6224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_531031, whole genome shotgun sequence.
ACCESSION   DATCRO010000201 DATCRO010000000
VERSION     DATCRO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531031"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VIN75_05400"
     CDS             complement(<1..642)
                     /locus_tag="VIN75_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973634.1"
                     /translation="MGRAAALLRGLSLATLVGWSAAGAPSSFAAGFPEAGLAIPTRDD
                     EIVEQLPSHVGDAAQRRAARAAERAQRAQLQAHPDELALALQAARDALARGRRRGDPR
                     ELGTVEAALAPWWSLAEPPPEVRLVRATVRQSQHEFQAALADLDALLALPALALPVRA
                     QAELTRAGVQQVLGHLADAAAGCRRLAGPAFIAVGGDAHRNALACLAELASLQG"
     gene            complement(645..911)
                     /locus_tag="VIN75_05405"
     CDS             complement(645..911)
                     /locus_tag="VIN75_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973635.1"
                     /translation="MTRFPVLAALAASVAAGGLLAGCGGDSTPPAPAPPAASNELPAS
                     AVASDSALVAFALADSPSEVADPLMLDQVATFPTSETDEPIALP"
     gene            complement(931..2520)
                     /locus_tag="VIN75_05410"
     CDS             complement(931..2520)
                     /locus_tag="VIN75_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY8973636.1"
                     /translation="MRSLSLPASTVAPLAAATLLALAGVAHASSHREAPFISGAPKVD
                     ASDFYMFNSYEAGRSDYVTLIANYQPLQSGYDGPNYHAMDPNALYEIHVDNDGDGKED
                     LTFQFRFKSTVANNGAGLTVPVGNVQQEIAPMQNGVVSMPNDPHLQVNESYTVTLVRG
                     DRRTGSAAALTNASGGAGTFAKPFDDIGEKTIPNYAAYAAAHVYDVNIPGCSGAGRVF
                     VGQRQDPFAVNLGVVFDMVDAPVGALLDPSAIGASSSTLDEFNVTSIALEVPKACLVA
                     GSDPVIGAWTTASLRQARVLDPSPRPGWQTTDREGGAWTQVSRLGNPLVNELVIGLKD
                     KDRFNGSKPRDDTQFLSYVTNPSYPKLLESYLGAPGIAPTNIPRNDLVTVFLTGIAGV
                     NKPAAGAPAEEMRLNTAIAAVPFAQQNRLGIVGNILAGGSDNAGYPNGRRPKDDIVDI
                     TLVAAMGGLCVANGTGNALGLGADCNPSAVPLGADVFKLNDNVDQATRPFLSAFPYLN
                     TPVPGTGPMAAAATARRGASK"
     gene            complement(2707..3588)
                     /gene="argP"
                     /locus_tag="VIN75_05415"
     CDS             complement(2707..3588)
                     /gene="argP"
                     /locus_tag="VIN75_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator ArgP"
                     /protein_id="HEY8973637.1"
                     /translation="MLDRDQLETFATIVELQSFERAAGALRITRGAVSQRIKALEQSL
                     AAVLLVREKPVVATPAGQTLLRHVQALRMLEGSTLRELGPGAVPGEPVAMAVSINADS
                     LATWFRGVLWALLRERGVALEVIVDDLDHTAARLARGQVVGCVSSDAAPASGFAAKAL
                     GEMEYRCYASPAFAREFFPAGLALPAVLAVPTVLFDRKDSLHDRYLARCLGFAVDRYP
                     RHYLPSPDALMEAIEQGVGYGLLPSRQAAARVAAGVLEELPPHVAVPVALHWHHWEFE
                     PPLCKALTEMVVRHALA"
     gene            complement(3631..4650)
                     /gene="argF"
                     /locus_tag="VIN75_05420"
     CDS             complement(3631..4650)
                     /gene="argF"
                     /locus_tag="VIN75_05420"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8973638.1"
                     /translation="MAIAPAHRDAALALATRRDHERRALRPRSFITLDDIDARALSQV
                     VDQALAAKRGLADGAGALAGRSVALLFQKTSTRTRCSFAAAARESGAQADYLDWSRSN
                     FVLAELRDEIAVLSRYYDLIVARVDRHESLRVMAAHSEVPVVNGLCDRHHPCQAVSDV
                     MTLKEYFGADLRGLRLAWLGDGNNVCRSLVQAATRSGVEVTLSSPAGHELDDVTACQG
                     GALVRRVADPRAAVEGADVVYTDTWVSMGQEAELAARKAALAGWQVDGRLMDAAPRHA
                     LFMHCLPAHPGLEVTAGVLRSPRSIVLDQAENRKHAQKALLAWLLRANARTAARPAPA
                     TTLAA"
     gene            complement(4796..5029)
                     /locus_tag="VIN75_05425"
     CDS             complement(4796..5029)
                     /locus_tag="VIN75_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973639.1"
                     /translation="MRYGPTDQQILQRLDDLVARTMQRCADLERTIAVGMASGVDTAA
                     ADVALESSYRALALFRHKLAEVERRTGHRAAGN"
     gene            complement(5010..5306)
                     /locus_tag="VIN75_05430"
     CDS             complement(5010..5306)
                     /locus_tag="VIN75_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973640.1"
                     /translation="MANALTTTQIEILRRLRDGIDLVGEPWLSGLVGDVSFLRHLGLV
                     DLQGIFNVSLSENGRLYLSAAELESRESHASGLAGPLQEQPRDTEGPDDAIRPH"
     gene            5922..>6224
                     /locus_tag="VIN75_05435"
     CDS             5922..>6224
                     /locus_tag="VIN75_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8973641.1"
                     /translation="MKPTFSLIALAAIAPAAFAQSSVTIYGIVDLDGQYLSGHAKQVL
                     VTSGGQSGSRIGFKGSEDLGSGYFADFVLEGGLNVDTGGSGQGGALFGRQAFGALRT"
BASE COUNT          858 a         2160 c         2317 g          889 t
ORIGIN      
        1 cccctgcagg cttgcgagtt cggccaggca ggcgagcgcg tttcggtgcg cgtcgccgcc
       61 caccgcgatg aacgccggac ccgccaggcg ccggcagccg gccgcggcgt cggccagatg
      121 gcccagcacc tgctgcacgc ccgctcgcgt gagctcggcc tgcgcgcgca ccggcaacgc
      181 cagcgcgggc agcgccagca aggcgtcgag gtcggccagc gcggcctgga actcgtgctg
      241 gctctggcgg acggtcgccc gcaccaggcg cacctcgggc ggcggctccg cgagcgacca
      301 ccagggcgcg agcgcggcct cgacggtgcc gagctcgcgc gggtcgcccc gccggcggcc
      361 gcgggccagc gcgtcgcgcg cggcctgcag cgccagcgcg agctcgtccg gatgcgcctg
      421 cagctgcgcg cgctgcgccc gttcggccgc gcgcgcggcg cgacgttgcg cggcgtcgcc
      481 gacgtgcgac ggcaactgct cgacgatctc gtcgtcccgc gtgggaatcg ccaggccggc
      541 ctcgggaaag ccggcggcga acgacgaagg cgcacccgcc gccgaccacc cgacgagggt
      601 cgccagcgac aggccgcgca acagggcggc ggcgcggccc atcgtcaggg cagtgcgatg
      661 ggctcgtcgg tctcgctcgt cgggaacgtc gcgacctggt cgagcatcag cggatcggcc
      721 acctcgctcg gggaatccgc gagcgcgaag gccacgagcg cgctgtcgga ggcgacggcg
      781 ctggcgggca gttcgttgga cgccgcaggc ggcgcaggcg cgggcggggt gctgtcgccg
      841 ccgcagccgg cgagcaggcc gccggcggcc acggacgcgg cgagcgcggc gaggacggga
      901 aatcgggtca tggtgggttc ctccatgtgc tcacttggac gcgccgcggc gggcggtggc
      961 ggccgcggcc atcgggcccg tgcccgggac cggcgtgttc aggtacggga atgccgacag
     1021 gaacggccgc gtcgcctggt ccacgttgtc gttcagcttg aacacgtccg cgcccagcgg
     1081 gacggccgac gggttgcagt ccgcgcccaa gcccagcgcg ttgcccgtgc cgttggccac
     1141 gcacagtccg cccatcgcgg cgaccagcgt gatgtcgacg atgtcgtcct tgggccggcg
     1201 gccgttggga tagccggcgt tgtcgctgcc gcccgccagg atgttgccga cgatgcccag
     1261 ccggttctgc tgcgcgaacg gcaccgcggc gatcgcggta ttgaggcgca tctcctcggc
     1321 gggcgcgccg gcggccggct tgttgacgcc ggcgatgccc gtgaggaaca cggtcacgag
     1381 gtcgttgcgc gggatgttgg tcggcgcgat gcccggcgcg ccgaggtagc tctcgagcag
     1441 cttcgggtac gacgggttgg tgacgtagct gaggaactgc gtgtcgtccc tgggcttgga
     1501 cccattgaag cggtccttgt ccttcaggcc gatgaccagc tcgttgacga gcgggttgcc
     1561 caggcgcgag acctgcgtcc aggcgccgcc ctcgcggtcg gtcgtctgcc agccgggcct
     1621 gggcgacgga tcgagcacgc gcgcctggcg caggctcgcg gtggtccagg cgccgatgac
     1681 cggatcgctg ccggcgacca ggcaggcctt gggcacctcg agcgcgatcg acgtcacgtt
     1741 gaattcgtcg agcgtgctgg acgaggcgcc gatcgcgctc gggtcgagca gcgcgccgac
     1801 cggcgcgtcc accatgtcga acacgacgcc cagattgacc gcgaacggat cctgccgctg
     1861 gcccacgaac acgcggccgg cgccgctgca gccggggatg ttgacgtcgt agacgtgcgc
     1921 ggccgcgtac gccgcatagt tggggatcgt cttctcgccg atgtcgtcga acggcttggc
     1981 gaaggtgccg gcgccgccgc tggcgttggt gagcgcggcg gcgctgcccg tgcggcggtc
     2041 gccgcgcacg agcgtcaccg tgtacgactc gttgacctgc aggtgcgggt cgttgggcat
     2101 ggagaccacg ccgttctgca tcggcgcgat ctcctgctgc acgttgccca ccggcaccgt
     2161 caggccggcg ccgttgttgg cgaccgtcga cttgaagcgg aactggaacg tgaggtcctc
     2221 cttgccgtcg ccgtcgttgt cgacgtggat ctcgtagagc gcgttcggat ccatcgcgtg
     2281 gtagttcggg ccgtcgtagc cgctctgcag gggctggtag ttggcgatca gcgtcacgta
     2341 gtcggagcgg cccgcctcgt agctgttgaa catgtagaag tccgacgcgt ccaccttggg
     2401 cgcgccggag atgaaggggg cttcgcggtg gctggaggcg tgcgcgacgc cagccagggc
     2461 cagcagggtc gcggccgcca ggggcgcgac ggtcgacgcg ggcagggaaa gggatcgcat
     2521 gtcttggctc ctgtgggttg tcgtgatggg cgccgccgtg aggcaggcca tcccgactac
     2581 gcacgcagcc ggcgccgtgg atgcacgccg cgccttgtcc cccttgcgcg cagagccttg
     2641 catccgcgcg gcgccgcggc acgtaggagc cggcgccagg cgcccgcggg gcgggcctcg
     2701 gccgggtcag gccagcgcgt gacgcacgac catctcggtc agcgccttgc acaggggcgg
     2761 ctcgaattcc cagtgatgcc agtgcagcgc cacgggcacc gccacgtgcg gcggcaactc
     2821 ctccaggacg ccggcggcga cgcgcgcggc cgcctgccgg ctgggcagca ggccgtagcc
     2881 caccccttgc tcgatcgcct ccatcagcgc gtccggcgac ggcaggtagt ggcgcgggta
     2941 gcggtcgacg gcaaagccca ggcagcgcgc gaggtagcgg tcgtgcagcg agtccttgcg
     3001 gtcgaacagc acggtgggca cggccagcac cgcgggcagc gcgaggccgg cggggaagaa
     3061 ctcgcgcgcg aaggcggggc tggcgtagca gcgatattcc atctcgccga gcgccttggc
     3121 cgcgaagccc gatgccggcg ccgcgtcgga gctgacgcat ccgacgacct gtccgcgggc
     3181 caggcgcgcg gcggtgtggt cgaggtcgtc gacgatcacc tccagcgcca cgccgcgctc
     3241 gcgcagcagc gcccacagca cgccgcggaa ccaggtggcg agcgagtccg cgttgatgct
     3301 gacggccatg gccaccggct cgcccggcac ggcgcccggg cccagctcgc gcagcgtcga
     3361 gccctcgagc atgcgcagcg cctgcacgtg gcgcagcagg gtctggccgg cgggcgtcgc
     3421 gacgacgggc ttctcgcgca ccagcagcac ggccgcgagc gactgctcca gtgccttgat
     3481 gcgctgcgac accgcgccgc gcgtgatgcg cagcgcgccg gccgcgcgct cgaagctctg
     3541 gagttcgacg atggtcgcga aggtctcgag ttggtcccgg tcaagcaagc ggcctgcccc
     3601 tggtggttgg ctggctcgat gatggcatgg tcaggccgcc agcgtggtcg cgggcgcggg
     3661 acgcgcggcc gtgcgggcgt tcgcgcgcaa gagccaggcc agcaaggcct tctgcgcgtg
     3721 cttgcggttc tcggcctggt ccaggacgat ggatcgcgga gagcgcagca cgccggccgt
     3781 cacctccagc ccggggtggg caggcaggca gtgcatgaac agcgcgtggc gcggggcggc
     3841 gtccatcagt cgcccgtcca cctgccagcc ggccagcgcc gccttgcgcg ccgcgagctc
     3901 ggcctcctgg cccatcgaga cccaggtgtc cgtgtagacg acgtccgcgc cctcgacggc
     3961 cgcgcgcggg tcggccacgc gccgcaccag cgcgccgccc tggcaggccg tcacgtcgtc
     4021 cagctcgtgg ccggcgggcg acgacagcgt cacctcgacg cccgagcggg tcgcggcctg
     4081 cacgagcgag cggcagacat tgttgccgtc gccgagccag gccaggcgca ggccgcgcag
     4141 gtcggcgccg aagtactcct tgagcgtcat cacgtccgag acggcctggc agggatggtg
     4201 gcggtcgcac agcccgttga cgacgggcac ctcgctgtgg gccgccatca cgcgcagcga
     4261 ttcgtggcgg tcgacgcggg cgacgatcag gtcgtagtag cgcgagagca cggcgatctc
     4321 gtcacgcagc tcggccagca cgaagttcga gcgcgaccag tcgaggtagt cggcctgcgc
     4381 acccgactcg cgggccgcgg ccgcgaacga gcagcgggtg cgcgtcgacg tcttctggaa
     4441 cagcagcgcg accgagcggc ccgccagcgc gccggcgcca tcggccaggc cgcgcttggc
     4501 cgccagggcc tggtcgacca cctgggacag cgcgcgcgca tcgatgtcgt ccagcgtgat
     4561 gaaggagcgc ggacgcagcg cgcggcgctc gtggtcgcgg cgggtggcca gtgccagggc
     4621 ggcgtcacgg tgggcagggg cgatggccat gcggcaggtc ctttcgtcgg gaggaaggcg
     4681 cgactttagg agctgggcgc gcgggcggcg cacggttcag aaaatgtgcc ggggcgtcag
     4741 ggccgctaac cagcatcgcg ccgtccgcgg cgatggcccg atgcaggccg gcgagtcagt
     4801 tgccggcggc gcgatggccc gtgcgccgtt ccacttccgc gagcttgtgc ctgaacaacg
     4861 cgagcgcccg gtagctcgac tccagggcga cgtcggcggc cgcggtgtcg acgccgctcg
     4921 ccatgcccac cgcgatggtg cgctcgaggt cggcgcagcg ctgcatggtc cgggcgacca
     4981 ggtcgtccag ccgctgcagg atctgttggt cagtggggcc gtatcgcatc gtcggggcct
     5041 tcggtgtccc gaggctgctc ctgcagcggg ccggccaggc ccgacgcgtg gctctcccgc
     5101 gattccagtt ccgcggccga caggtacagg cgtccgttct cgctgaggga gacgttgaag
     5161 atcccctgca ggtcgaccag gccgagatgc cgcaggaacg agacgtcgcc gaccaggccc
     5221 gacaaccacg gttcgccaac cagatcgatc ccatcgcgca acctgcgcag gatctcgatc
     5281 tgcgtcgtcg tcaatgcgtt cgccattgca ctttcccgcc ggtgagcgga accagccatg
     5341 gtgtcaggac actctcgccg ctcgaggcgg caggtgcgcg gcgcgacacc ctctgcgcca
     5401 cgctctttga agtcattgtg ttcgcgatct gccttgcgtg gatgagcttt cgacgtggaa
     5461 agccgcaact ttgcttaggc ggagagaact gccgcatcgg ttcccgaatg catgggtttc
     5521 catccactcg acagtgggtg gacggccgcg ttcgcatgcc agcgtgtgga ttccattgct
     5581 ttgccgcgaa ggctcatgtc gtgaggcgcg tatgcacggt ggcgaaagtc caccgcgtcc
     5641 cgaccccgcc ttggcgggat cccgccgacg tggcgcatgc acgcatcgag agcctgcgcg
     5701 agcctctcgc gggggccacg acgggaacga ggggtgcagg cggagtgtca tgcagtcgcg
     5761 gagtccgttg gcacagctca tgcagtcggc gtgcctggtc tttcaaccaa aacacggaga
     5821 catgcatgag tgacaggaca tcggtgaaca cgcatccgga gcgggcacgc gccccttccg
     5881 gttcggacca ggtcaccgct ctcaacttcc aacgacattt catgaagccc accttcagcc
     5941 tcatcgccct ggccgccatc gcgccggccg ccttcgcgca gtcgagcgtc accatctacg
     6001 gcatcgtcga cctcgacggc cagtacctgt ccggccacgc caagcaggtg ctggtcactt
     6061 ccggcggcca gtccggcagc cgcatcggct tcaagggcag cgaggatctg ggctcgggct
     6121 actttgccga cttcgtgctc gaaggcggcc tgaacgtgga caccggcggc tccggccagg
     6181 gcggggcgct gttcggccgc caggccttcg gcgcgctgcg cacc
//
LOCUS       DATCRO010000202         4973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_137168, whole genome shotgun sequence.
ACCESSION   DATCRO010000202 DATCRO010000000
VERSION     DATCRO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137168"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VIN75_05440"
     CDS             complement(<1..608)
                     /locus_tag="VIN75_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8973642.1"
                     /translation="MNAPERLPVPAEPDAKALAAFSETAWNARILPQLKDYVAIPAKS
                     PMFDADWEKHGLLDKVVRDAASWVEGRKVPGLTLEVIRQPGRTPVIFFEVEATRPSSF
                     GTPPTVVMYGHLDKQPEFSGWRSDLGPWTPKVEDDKLYGRGGADDGYAVYAAVSAIEA
                     LKAQGTTHPRIVGLIESCEESGSGDLPVYLDLLKDRLGDVSLV"
     gene            complement(799..1653)
                     /locus_tag="VIN75_05445"
     CDS             complement(799..1653)
                     /locus_tag="VIN75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HEY8973643.1"
                     /translation="MKPVTTDDGLALHLRTWPAANPSRGVAVLVHGLGEHIDRYDHVA
                     RRLNGLGFTVVGYDHRGHGRSPGQRGGIPADDTLCADLGRVLRAARESVPGPLTLVGH
                     SLGGLIAGRFVAEGLSATPAPWWCRVDALVMSSPALDPGTNAIQKLLLAVVAPMLPNL
                     AVNNGLKVDWISRDAGVVKAYADDPLVHDRITGRLGLFVARQGPAVIAAAPRWKTPTL
                     LMWAGADRCVAPAGSAAFAAGAPRDVVTVREWPGLFHEIFNEPEQGQVLQALAGWLEA
                     HVPETIPA"
     gene            1774..2319
                     /locus_tag="VIN75_05450"
     CDS             1774..2319
                     /locus_tag="VIN75_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973644.1"
                     /translation="MTNVSPITFRAARPDDALCLGVLSTQVFLHTYTKHGIRPALARE
                     VLANHSVARYEALLADPDKTIVLAECAGHLVALSLVDNAARLEGRPELVAAKLEHLYV
                     QEPFTGRGIGRDLLRHAEKAAAARGAEMLWLTAWVGNQRALSFYPRCGYDDLGEVLYT
                     FEGESHPNRLFGKRVRHVAPA"
     gene            2368..3210
                     /locus_tag="VIN75_05455"
     CDS             2368..3210
                     /locus_tag="VIN75_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8973645.1"
                     /translation="MNDFFRLDHDAASGVAELVLNRPDRMNTMAPAFFPALRDAVQAL
                     DDAGDTRALLLRGEGKHFSAGMALDTFAPDGPMQAMLDTGSARKRLAFQTQLRRLMAC
                     FDVLDAARFPVVCAVQGACVGGALDLAAACDLRVCSADAFFTVQEIHIGMAADLGVLQ
                     RLQRLLPQGLARELAYTGDRLGAERALAAGFVNAVLPDADALVAHARRLAAAIAAKSP
                     LAIAASKSALNHARDHSTAASLEQMTLLQSAVFDIDEMARAIAAWQAKTAATFDPLAP
                     IARP"
     gene            3347..3775
                     /locus_tag="VIN75_05460"
     CDS             3347..3775
                     /locus_tag="VIN75_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006295980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY8973646.1"
                     /translation="MATTAPGYSGTPLARKLGIVAGSRVAPLHAPDDYLDWLAPLPGG
                     VRFAAKVSATTDVVHVFADRRADLAACLQDLRGRIAPAAAVWVSWPKKASKVPTDITE
                     DTIREIALPLGYVDVKVCAVSDVWSGLKLVIRKELRGQPT"
     gene            3883..4173
                     /locus_tag="VIN75_05465"
     CDS             3883..4173
                     /locus_tag="VIN75_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973647.1"
                     /translation="MNFRTLSIACALIGAVSFAGAAEVADAAASAASAVKHAAKATGH
                     AVAKGASAAATGTEHAASAVATGTAHAAHKAASGVKHAAHKAASAASEAVTK"
     gene            4280..4576
                     /locus_tag="VIN75_05470"
     CDS             4280..4576
                     /locus_tag="VIN75_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973648.1"
                     /translation="MLPVNRASVLLAAALAVASPLAGGALAWAFVDERTRGLGYHLLY
                     WPVLLIGLLPRRASDVLMASGLVPLLLYFGGYLLACHAVRLLWRRVRRDGRAAS"
     gene            4573..>4973
                     /gene="yidD"
                     /locus_tag="VIN75_05475"
     CDS             4573..>4973
                     /gene="yidD"
                     /locus_tag="VIN75_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8973649.1"
                     /translation="MSVPRTLLLAAIRGYKRHVSPRKGFACAHRIHLGGCSCSTLGLR
                     AVSRYGAWRGLGVLRLRLRECHLLAEQLRARPAAVRASQAGFVDCDVPCDGSCIDASS
                     CTPGDACDATDACEIISCIDWGDCGCGDWLSS"
BASE COUNT          642 a         1833 c         1796 g          702 t
ORIGIN      
        1 accagcgaca cgtcgcccag gcgatccttg agcaggtcga ggtagacggg caggtcgccc
       61 gagcccgatt cctcgcagct ctcgatcagg ccgacgatgc gcgggtgcgt cgtgccctgg
      121 gccttgagcg cctcgatggc cgagaccgcc gcatagaccg cgtagccgtc gtccgcgccg
      181 ccgcggccgt agagcttgtc gtcctcgacc ttgggcgtcc agggccccag gtcgctgcgc
      241 cagccgctga actcgggctg cttgtccagg tggccgtaca tgacgaccgt gggcggcgtg
      301 ccgaacgacg aggggcgcgt ggcctcgacc tcgaagaaga tgaccggcgt gcggcccggc
      361 tggcggatga cttccagcgt caggcccggc accttgcggc cctcgaccca cgacgcggca
      421 tcacgcacga ccttgtcgag caggccgtgc ttttcccagt cggcgtcgaa catcgggctc
      481 ttggccggga tggcgacgta gtccttgagc tgcggcagga tgcgcgcgtt ccaggccgtc
      541 tcggaaaagg cggccagcgc cttcgcatcg ggctcggcgg ggacgggcag gcgttcgggg
      601 gcgttcatgg cgggctccaa tgggcgaccc gggtgcggtc agcggccgtg gcccgcgggt
      661 ggcggacgag gccctgcgcg ccgggtggcg ggattcaggg tagaccgcca ttctgcgcca
      721 gtcgcccccg cgagtccgag cgccaaaccg cgtcgaaacc gaggatttcg ctaccggcgg
      781 cgccccggcg ccgcggcttc aggccggaat cgtctccggc acgtgggcct cgagccagcc
      841 ggccagcgcc tgcagcacct ggccctgttc cggctcgttg aagatctcgt ggaacaggcc
      901 gggccattcg cgcacggtga cgacgtcgcg cggcgcgccc gcggcgaaag cggcgctgcc
      961 cgccggcgcg acgcagcggt cggcgccggc ccacatcagc agcgtgggcg tcttccagcg
     1021 cggcgcggcg gcgatcaccg ccgggccctg gcgcgccacg aacaggccca ggcggccggt
     1081 gatgcggtca tgcaccagcg ggtcgtcggc gtaggccttg acgacgccgg cgtcgcgcga
     1141 gatccagtcg accttcagcc cgttgttgac cgccaggttg ggcagcatcg gcgcgacgac
     1201 cgccagcagc agcttctgga tcgcgttggt gcccggatcg agcgcgggcg aggacatcac
     1261 cagcgcgtcg acgcggcacc accacggcgc gggcgtcgcc gacaggcctt cggcgacgaa
     1321 gcgtcccgcg atcaggccgc ccaggctgtg gccgaccagc gtcagcgggc cgggcacgct
     1381 ctcgcgcgcg gcgcgcagca cgcggccgag gtcggcgcac agcgtgtcgt cggcggggat
     1441 gccgccccgc tggccgggcg aacggccgtg gccgcgatgg tcgtagccga ccacggtgaa
     1501 gcccaggccg ttcaggcgcc gggcgacgtg gtcgtagcgg tcgatgtgct cgcccagccc
     1561 gtgcaccagc acggcgacgc cgcggctcgg gttggctgcg ggccaggtgc gcaggtgcag
     1621 ggcgaggccg tcgtcggtgg tcacgggttt catgcggcgc agtgtagggg ggcgccccgc
     1681 ctccggccat ccggatcggc acctgcaggc gcgctatgct gcgtggctgc aacgacggcg
     1741 gcgccatcgc gccgccgcga ccttccggaa cgcatgacga acgtctctcc catcacattc
     1801 cgggccgccc ggcccgacga cgccctgtgc ctgggcgtgc tgtccacgca ggtcttcctg
     1861 cacacctaca cgaagcacgg catccgtccc gcgctggcgc gcgaggtgct cgccaaccac
     1921 tcggtcgccc gctacgaggc gctgctcgcg gatccggaca agacgatcgt cctggccgaa
     1981 tgcgcggggc acctggtcgc cctgtcgctg gtggacaacg ccgcgcgcct cgaaggccgg
     2041 cccgaactcg tggcggccaa gctcgagcac ctgtacgtgc aggagccgtt caccggccgc
     2101 ggcatcggcc gcgacctgct gcgccacgcc gagaaggccg ccgcggcgcg cggcgccgag
     2161 atgctgtggc tgaccgcctg ggtcggcaac cagcgcgcgc tgagcttcta tccgcgctgc
     2221 ggctacgacg acctcggcga ggtgctgtac accttcgagg gcgagtcgca tcccaaccgc
     2281 ctgttcggca agcgcgtgcg ccacgtggcg ccggcctgag aaggcgcgcc cgccgatacc
     2341 ccgggccagg ccaccggagc gaacgccatg aacgacttct tccgcctcga ccacgatgcc
     2401 gccagcggcg tcgccgagct cgtgctgaac cggcccgacc gaatgaacac gatggcgccg
     2461 gcgttcttcc ccgcgctgcg cgacgccgtg caggcgctcg acgacgccgg cgacacgcgc
     2521 gcgctgctgc tgcgcgggga aggcaagcac ttcagcgccg gcatggcgct ggacaccttc
     2581 gcgcccgacg gcccgatgca ggcgatgctg gacaccggca gcgcgcgcaa gcgcctcgcg
     2641 ttccagaccc agctgcgccg cctgatggcc tgcttcgacg tgctggacgc cgcgcgcttc
     2701 ccggtcgtct gcgcggtgca gggcgcgtgc gtgggcgggg cgctggacct cgcggcggcc
     2761 tgcgatttgc gtgtctgcag cgccgacgcg ttcttcacgg tgcaggagat ccacatcggc
     2821 atggccgccg acctgggcgt gctgcaacgc ctgcagcgcc tgctgccgca gggcctcgcg
     2881 cgcgagctcg cgtacacggg cgaccgcctg ggcgcggaac gcgcgctggc cgcgggcttc
     2941 gtcaacgcgg tgctgccgga cgccgacgcg ctggtcgccc acgcgcgccg gctcgcggcg
     3001 gccatcgcgg ccaagtcgcc actggcgatc gcggccagca agtcggcgct gaaccacgcg
     3061 cgcgatcatt cgacggccgc gtcgctggag cagatgacgc tgctgcagag cgcggtgttc
     3121 gacatcgacg agatggcgcg cgcgatcgcg gcgtggcagg ccaagacggc ggccaccttc
     3181 gatccgctgg cgccgatcgc ccggccctga gcggatccgc ggcgatgagc gtcctgccca
     3241 agcacgtcgc cgccgtgggc tgcgagggca ccggacactt ccgcgtcacg cgcgacttcc
     3301 tgctgcaccg ccacaaggtg ttcgacatcc tgatggagtc ctgagcatgg ccacgaccgc
     3361 ccccggctac tccggcaccc cgctcgcccg caagctgggc atcgtcgcgg gcagccgcgt
     3421 ggcgccgctg catgcgcccg acgactacct ggactggctg gcgccgctgc ccgggggcgt
     3481 gcgcttcgcg gccaaggtgt cggcgacgac ggacgtcgtc cacgtgttcg ccgatcgccg
     3541 cgccgacctg gcggcctgcc tgcaggacct gcgcgggcgc atcgcgccgg cggcggcggt
     3601 gtgggtgtcc tggccgaaga aggcgtccaa ggtgcccacc gacatcaccg aggacacgat
     3661 ccgcgagatc gcgctgccgc tcggatatgt cgacgtcaag gtgtgcgcgg tcagcgacgt
     3721 ctggtccggg ctgaagctgg tgatccgcaa ggagctgcgc ggtcaaccga cctgacgggt
     3781 gtcgcgttgg ttgctggcag gcgtccccga aggcgccggc atcgcggcgc ggcgtgtccc
     3841 aaggcaccag cgcgttggtc cgacaactcg atggaaatca ccatgaactt ccgtactctc
     3901 tcgatcgcct gcgccctgat cggtgccgtt tccttcgccg gcgccgccga agtcgccgac
     3961 gccgcggcca gcgccgccag cgcggtcaag cacgccgcca aggccaccgg ccacgccgtc
     4021 gccaagggcg cgtcggccgc cgcgaccggc accgagcacg ccgcgtcggc ggtggccacc
     4081 ggcaccgcgc acgccgcgca caaggccgcc agcggcgtca agcacgccgc ccacaaggcc
     4141 gcgtcggccg cgtcggaagc cgtcaccaag taagcggttc cctcccaggg ccccggttcc
     4201 ccatggaccg gggccttgtc gtttccggcg cgcgcgtggc gatcggcggt cggcaggcga
     4261 gccgtcacaa tggcgaccca tgcttcccgt caatcgcgcc agcgtcctcc ttgccgccgc
     4321 tttggccgtg gcctcgccgc tggccggcgg cgcgctggcg tgggccttcg tcgacgagcg
     4381 cacgcgcgga ctgggctacc acctgcttta ctggccggtc ctgttgatcg gcctgctgcc
     4441 gcgccgggcg agcgatgtcc tgatggcctc ggggctggtg ccgctgctgt tgtacttcgg
     4501 cggctatctg ctggcgtgcc acgccgtgcg cctgctgtgg cgacgcgtcc gccgcgacgg
     4561 ccgggccgcc tcgtgagcgt tccgcgcacg ctgctcctgg ctgccatccg cggctacaag
     4621 cgccacgtgt cgccacgcaa gggcttcgca tgcgctcatc gcatccacct gggcggctgc
     4681 agctgctcca cgttggggct gcgcgcggtc tcgcgctacg gcgcgtggcg cggcctgggc
     4741 gtgctgcgcc tgcgcctgcg cgaatgccac ctgctggccg agcaattgcg cgcgcgaccc
     4801 gccgccgtcc gcgcgtcgca ggccggcttc gtcgattgcg acgtgccgtg cgacggctcc
     4861 tgcatcgacg cctcctcgtg cacgccgggc gatgcctgcg atgcgacgga cgcgtgcgag
     4921 atcatcagct gcatcgactg gggcgactgc ggctgcggcg actggctgtc ctc
//
LOCUS       DATCRO010000203         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_801035, whole genome shotgun sequence.
ACCESSION   DATCRO010000203 DATCRO010000000
VERSION     DATCRO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801035"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..985
                     /locus_tag="VIN75_05480"
     CDS             83..985
                     /locus_tag="VIN75_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973650.1"
                     /translation="MTAPTGIGQYVRPQNQAQGDRVQAQQMVDRPMLLWVKDVKHINR
                     TQYQADGGDGVIVDFIDLQTNTPYIGVMWMAGAIVDGLRPFVEDGNAYPVMIKKQKGG
                     KFGYYNAIEPITEQPWLDHVTANFQTFVNLIAQTRAAKEQEWAAAMAQQNQPPAPPAP
                     PAGPPTMTPMQVAANPQAGLYDQAMAMQPPHVQQALQTAPAAPAPVQQPPAPAAPPAA
                     APAAVAPPQAPAAPAAPPQQYAAPAYAPPAAPAAPSAPVAPPQAPAAPGVPAPPAAAP
                     AAAPPGQMTNTSVAALQAQLDALG"
     gene            1092..>2363
                     /locus_tag="VIN75_05485"
     CDS             1092..>2363
                     /locus_tag="VIN75_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973651.1"
                     /translation="MRSKSWVPDDGNNGVPDLVDSFEFAAQAFEQQSIPISEFYDDPV
                     GFVREFVDFSDSVTGAERGLTDYQDEIMDAVPKHGRICVRGPHGLGKTTTNALTVLWF
                     AITREDAGRDWKVATTAGAWRQLEHYLWPEIKKWSRKLRWDKLGMTPWREGTSGELLS
                     LSLNLKHGSAFAVASSDEKKIEGVHADSVLYIFDESKAIRPQVFEAAEGAFSGASKST
                     GKRPSAPPAVRGMEAYAVATSTPGEPQGHFYEIHMNPDKFPDWKRLHVTLKQVIHAGR
                     ISQDWAEARKNQWGVDSAPYQNRVLGEFYASAADAVIPLAWVEDAIERWKVWEKSGRR
                     KHAPTKVLGVDVALGGTDFTGIAHRCGHVVEKVEKLTLSNTVKIAKEVKARQRPADES
                     VVDAIGVGAGVLATLRSWGLRARPFTASKKSF"
BASE COUNT          410 a          853 c          789 g          311 t
ORIGIN      
        1 ggttgagccc tggcgaagac accgggatga cctgtcccgg caacctgaaa acgagctagg
       61 ccgaaagaac ggaacgaacg acatgaccgc accgaccggc atcgggcagt acgtccggcc
      121 gcagaaccag gcgcagggcg accgcgtcca ggcccagcag atggtcgacc gacccatgct
      181 gctctgggtc aaggacgtca agcacatcaa ccgcacgcag taccaggcgg acggcggcga
      241 tggcgtcatc gtcgacttca tcgacctgca gaccaacacg ccgtacatcg gcgtgatgtg
      301 gatggccggc gcgatcgtcg acgggctgcg cccgttcgtc gaggacggca acgcctaccc
      361 ggtgatgatc aagaagcaga agggcggcaa gttcggctac tacaacgcga tcgagccgat
      421 caccgagcag ccgtggctag accacgtgac cgcgaacttc cagaccttcg tcaacctcat
      481 cgcgcagacc cgcgcggcga aggagcagga gtgggccgcg gcgatggcac agcagaacca
      541 gccgcccgcg cccccggccc cgcccgctgg cccgccgacg atgacgccga tgcaggtcgc
      601 cgcgaacccg caggccggcc tgtacgacca ggcgatggcg atgcagccgc cgcacgtcca
      661 gcaggcgctg cagacggctc cggccgcccc ggcgcccgtc cagcagccgc ccgcccccgc
      721 ggcgcccccg gcggcagctc cggccgccgt cgccccgccg caggctcccg cggccccggc
      781 cgctccgccg cagcagtacg cggccccggc ctacgcgccg cccgccgctc cggcggcacc
      841 ctcggcgccg gtcgccccgc cgcaggcccc cgctgcgcct ggcgtcccgg ctccgccggc
      901 ggccgctccg gccgccgcgc ccccggggca gatgacgaac accagcgtcg cggcgctgca
      961 ggcccagctc gacgcgctgg gttaagctga agactggtct catggtcggg acctgcgatg
     1021 gagccccgcc actcggcgtt cggaacccga gcggcggggc ttcgcgctga ccagccatta
     1081 cgatcagtag catgcggtcg aagagctggg tccccgacga cgggaacaac ggcgtccccg
     1141 acctggtcga ctccttcgag tttgccgctc aggctttcga acagcagtcc atcccgatct
     1201 ccgagttcta cgacgacccc gtgggcttcg tccgggagtt cgttgacttc tccgactcgg
     1261 tcaccggcgc cgagcgcggg ctcaccgact accaggacga gatcatggac gccgtgccga
     1321 agcacggccg gatctgcgtc cgcggcccgc acggtctcgg caagacgacc accaacgccc
     1381 tcaccgtgct ctggttcgcg atcacccgtg aagacgctgg gcgcgactgg aaggtggcca
     1441 ctaccgccgg cgcctggcgg cagctggagc actacctctg gcctgagatc aagaagtggt
     1501 cgcggaaact gcgctgggac aagctcggca tgacgccgtg gcgcgaaggc acctcgggcg
     1561 aactgctcag tctgtcgctg aacctcaagc acggcagcgc ttttgccgtc gccagctccg
     1621 acgagaagaa gatcgaaggt gtgcacgccg acagcgtcct gtacatcttc gacgagtcga
     1681 aggcgatccg gccgcaggtc ttcgaggccg cggaaggtgc cttctccggt gcgtcgaagt
     1741 cgacgggaaa acgtcccagc gcgccgccgg ctgttcgtgg catggaagcc tacgccgtcg
     1801 cgacgtccac gccgggcgag ccgcaaggcc acttctacga gatccacatg aacccggaca
     1861 agttcccgga ctggaagcgc ctgcacgtca cgctcaagca ggtcatccac gcgggtcgga
     1921 tctcgcagga ctgggccgaa gcccggaaga accagtgggg cgtcgactcc gcgccgtacc
     1981 agaaccgcgt cctcggcgag ttctatgcct cggccgccga cgccgtgatc ccgctcgctt
     2041 gggtcgagga cgcgatcgag cgctggaagg tctgggagaa gtccgggcgc cggaagcacg
     2101 cgccgacgaa ggtcctcggc gtcgacgtcg cgctcggcgg caccgacttc actggcattg
     2161 cgcaccggtg cggtcacgtc gtggagaagg tcgagaagct cacgctctcg aacacggtga
     2221 agatcgccaa ggaggtcaag gcgcggcagc ggcccgccga cgagtccgtt gtggacgcca
     2281 tcggcgtcgg cgccggcgtg ctcgcgaccc tgcggagttg gggtctgcgc gcccggccgt
     2341 tcaccgcgag caagaagtcg ttc
//
LOCUS       DATCRO010000204         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_609942, whole genome shotgun sequence.
ACCESSION   DATCRO010000204 DATCRO010000000
VERSION     DATCRO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609942"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..886)
                     /locus_tag="VIN75_05490"
     CDS             complement(260..886)
                     /locus_tag="VIN75_05490"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY8973652.1"
                     /translation="MTDSTTWPPALPLVAILRGLQPERALDVARVLFDAGFRALEVPL
                     NRPGAIAAIAAIVPQAPADACIGAGTVLETAQVDAVGDTGATLIVSPHFDAAIVRRAR
                     ERGMRTVPGVFTASEAFAAWRAGADALKIFPAEAMTHAGLTGLTTVLPPGLPLWPVGG
                     IVPGNLADWRRAGATGFGLGGGLFKPEFGVAEIASRARAYVDAWHASA"
     gene            complement(883..1836)
                     /locus_tag="VIN75_05495"
     CDS             complement(883..1836)
                     /locus_tag="VIN75_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY8973653.1"
                     /translation="MTAAFIGIDWGTTHRRAALVGADGTLLDERADDDGALACKGRFR
                     DSLEALVAGWPGATPRLPVVMAGMVGAAIGWQAVPYLEGGTSLSDLPRHLVPVAEAPA
                     GRRWFIAPGWCLRGDDGAVDVMRGEETQLFGALHVLGPAASDGCYVLPGTHSKWVRLR
                     GGRITELRTYMTGELFALLRQHGTLASAMQTSMATGPAGLQHDSVADSPDFVRGVAQA
                     ARGPVLTHALFGARARVVTGAMAPGAAAAYVSGLLIGAEWSDAQRLASRDEPARVIGE
                     PALAALHAACARLHGRELETLDARRIQLAAWRAMATEDIPA"
BASE COUNT          285 a          732 c          780 g          237 t
ORIGIN      
        1 gaggtgacgc agtcagtcta ggaacgcggc gcgcgccgcg cttccctaaa atcggggtgt
       61 gttgcgcgaa atgcgacagc cgcaagggtt cgaacgcggc catttgcccg gcatccaggc
      121 aggatgcgag agcggcaggc cgccatcggc tttcgacgag ccaggcggtc tccaggcgat
      181 gcaccgcgac gacggcggtg gcgccagccg gcgcttgctc aagggcgggc ttgtcccggc
      241 cttgaaatcc ccgctcgcat cacgcgctcg cgtgccacgc gtccacgtag gcccgggcgc
      301 gcgaggcgat ctcggcgacg ccgaactcgg gcttgaacag cccgccgccc aggccgaagc
      361 cggtggcacc ggcgcgacgc cagtcggcga ggttgccggg cacgatgccg cccaccggcc
      421 acagcggcag gcccggcggc agcaccgtgg tcaggccggt gagacccgca tgggtcatcg
      481 cctcggcggg gaagatcttg agcgcgtcgg cgcccgcccg ccaggccgcg aaggcctcgg
      541 acgcggtgaa cacgccgggc accgtgcgca tgccgcgttc gcgcgcgcgg cgcacgatgg
      601 cggcgtcgaa atgcggcgac acgatgagcg tcgcgccggt gtcacccacg gcgtcgacct
      661 gcgcggtctc cagcaccgtg cccgcgccga tgcaggcatc ggccggcgcc tgcggcacga
      721 tggcggcgat ggcggcgatc gcgccgggcc ggttcaacgg cacctcgagc gcgcggaagc
      781 cggcgtcgaa cagcacgcgc gcgacgtcga gggcgcgctc gggctgcagg ccgcgcagga
      841 tggcgaccag cggcagcgcc ggcggccagg tggtggaatc ggtcatgcgg ggatgtcctc
      901 ggtggccatg gcgcgccagg cggccagttg gatgcggcgc gcgtcgagcg tctcgagttc
      961 gcggccgtgc aagcgtgcgc aggcggcgtg cagcgcggcc agcgcgggct cgccgatgac
     1021 gcgcgccggc tcgtcgcgcg acgccaggcg ctgcgcgtcc gaccattcgg cgccgatcag
     1081 cagaccgctg acgtacgccg ccgccgcgcc gggcgccatc gcgccggtga cgacgcgggc
     1141 gcgggcgccg aacagcgcgt gcgtcagcac cgggccgcgc gcggcctgcg cgacgccgcg
     1201 cacgaagtcg ggggaatcgg cgacgctgtc gtgctgcagc ccggcggggc cggtggccat
     1261 cgacgtctgc atcgcgctgg cgagcgtgcc gtgctggcgc agcagcgcga acagctcgcc
     1321 ggtcatgtag gtgcgcagct cggtgatgcg gcccccgcgc agccgcaccc acttgctgtg
     1381 cgtgcccggc agcacgtagc agccgtcgga cgcggccggt ccgagcacgt gcagcgcgcc
     1441 gaacaactgg gtctcctcgc cgcgcatgac gtcgaccgcg ccgtcgtcgc cgcgcaggca
     1501 ccagcccggc gcgatgaacc agcggcggcc cgccggcgcc tcggcgaccg gcaccaggtg
     1561 acgcggcagg tccgacagcg aggtgccgcc ttcgaggtag ggcaccgcct gccagccgat
     1621 ggccgcgccg accatgcccg ccatgacgac cggcaatcga ggcgtcgcgc ccggccagcc
     1681 ggccacgagg gcctccagcg agtcgcggaa gcgccccttg caggccaggg cgccgtcgtc
     1741 gtcggcgcgc tcgtcgagca gcgtaccgtc ggcgccgacc agcgcggcac gacggtgcgt
     1801 cgtgccccag tcgatgccga tgaatgcagc ggtcatgtcg atattgtcgt ggggccggaa
     1861 agccccggct ccgtgtggga tgagatcagg gctgcgcggc ggcaggtcag tcaccggggc
     1921 cgccaaccac gcgtcatcct gcacgaagtc gcaggatccg cgcctggcgg accgcgtagt
     1981 ctccgggaga ggtggatcct gtgactgcgc gcacgatgac accgggtggg cgcg
//
LOCUS       DATCRO010000205         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_924972, whole genome shotgun sequence.
ACCESSION   DATCRO010000205 DATCRO010000000
VERSION     DATCRO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924972"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VIN75_05500"
     CDS             complement(<1..369)
                     /locus_tag="VIN75_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973654.1"
                     /translation="MRRFLPPVAALLALAGCAAPPPVGAALDEVRIDSRGFEAGGDAC
                     ADFSLTAAQARYFLARSVVVTARQQHDDWDILPCYVRGTARSASGVWHWEIRAGGTAT
                     LETPAGDTELRGCSDCDAVLG"
     gene            complement(591..1982)
                     /locus_tag="VIN75_05505"
     CDS             complement(591..1982)
                     /locus_tag="VIN75_05505"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY8973655.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARMFVARLAHEGALARTA
                     RVKAEMYGSLGATGKGHGSDKAVLLGLAGHEPDTVDIEQVPAILDAIRTQHRVTLNGS
                     REIAFDESADLVMHRRETLPFHANGMRFTAYDDHGAELENRVYYSVGGGFVVSDEVAA
                     DGSRQKVVAPDTTVLPHPFHSGDDLLRLCDEHGIGIAELMRRNERHWRTDAEIDAGLM
                     KIWKVMQDCVARGCRTEGILPGGFKVKRRAAQLYRDLTVNPEAAMRDPLMVMDWVNLY
                     ALAVNEENAAGGRVVTAPTNGAAGIVPAVLHYYARFVPGASDKGVVDFMLTAAAIGIL
                     YKENASISGAEVGCQGEVGVACSMAAAGLAAVMGGSPRQVENAAEIGMEHHLGLTCDP
                     VGGLVQIPCIERNAIASVKAINAARMALRGDGTHHVSLDKVIKTMRETGADMQTKYKE
                     TARGGLAVNIVEC"
     gene            complement(2084..>3092)
                     /locus_tag="VIN75_05510"
     CDS             complement(2084..>3092)
                     /locus_tag="VIN75_05510"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540766.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY8973656.1"
                     /translation="HKDDLAAMMITYPSTYGVFEPGVKALCALVHRYGGRVYVDGANM
                     NAMVGVAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVADLVPFLPAHRAAGLG
                     AEDGKRVGAVSAAPLGNAAVLPISWMYVRMMGAAGLRQATETAILNANYIAARLSHHY
                     DIHFSGNIAGIQGGGVAHECILDLRPLKDSSGITAEDVAKRLIDYGFHAPTLSFPVAG
                     TLMVEPTESEPLAELDRFCDAMIAIRSEIAKVEGGHWPREDNPLKNAPHTAQALLKGE
                     WPHAYSREDAAYPVPALRLGKYWSPVGRVDNVYGDRNLFCSCVPMSEFAEQAEAEAAK
                     A"
BASE COUNT          429 a         1062 c         1122 g          479 t
ORIGIN      
        1 gccgaggacc gcgtcgcagt cgctgcagcc gcgcagctcg gtgtcgccgg ccggcgtctc
       61 cagcgtcgcg gtgccgccgg cgcggatctc ccagtgccag acgccgctgg ccgagcgcgc
      121 cgttccgcgc acgtagcagg gcaggatgtc ccaatcgtcg tgctgctggc gcgcggtgac
      181 caccaccgag cgcgcgagga agtagcgggc ctgggccgcg gtcagcgaga aatcggcgca
      241 ggcgtcgccg ccggcctcga agccgcgcga gtcgatgcgc acttcgtcga gcgcggcgcc
      301 caccggcggc ggcgcagcgc agcctgcgag ggcgaggagg gcggccaccg ggggaaggaa
      361 tcgtcgcatg gacggctagt gtcgtgagtt ggaaattcgt tgaataggtc tgaagcgaag
      421 aaaagacggt gccgaattcc gtccagaacg gttgcagact aggcgcgccc gcgacacgat
      481 gccggttggc ctcgcgagcg ggcgcaacga cggatgcggc cgttctgggc ggaattcttc
      541 aacgaatttc caactcacga cactagtgtc gcccagtttg ccgtcgccgc tcaacactcg
      601 acgatgttga cggccagccc gccgcgcgcg gtctccttgt acttggtctg catgtcggcg
      661 cccgtctcgc gcatcgtctt gatgaccttg tccaggctca cgtggtgcgt gccgtcgccg
      721 cgcagcgcca tgcgcgccgc gttgatggcc ttgaccgagg cgatcgcgtt gcgctcgatg
      781 cacgggatct gcacgaggcc gcccaccggg tcgcaggtca ggcccaggtg gtgctccatg
      841 ccgatctcgg cggcgttctc cacctggcgc ggcgagccgc ccatgacggc ggcgaggccg
      901 gcggccgcca tcgagcacgc cacgcccacc tcgccctggc agccgacctc cgcgccggag
      961 atcgacgcgt tctccttgta gaggatgccg atcgccgcgg cggtgagcat gaagtcgacc
     1021 acgcccttgt cgctcgcgcc cggcacgaag cgcgcgtagt agtgcagcac ggccggcacg
     1081 atgcccgcgg cgccgttggt gggcgcggtg acgacgcggc ctccggcggc gttctcctcg
     1141 ttgacggcca gcgcgtacag gttgacccag tccatcacca tcaaggggtc ccgcatcgcc
     1201 gcttcggggt tgacggtgag gtcgcggtac agctgcgccg cgcggcgctt gaccttgaag
     1261 ccgccgggca ggatgccctc cgtgcgacag ccgcgcgcca cgcagtcctg catcaccttc
     1321 cagatcttca tcaggccggc gtcgatctcg gcgtcggtgc gccagtggcg ctcgttgcgg
     1381 cgcatcagct cggcgatgcc gatgccgtgc tcgtcgcaca ggcgcagcag gtcgtcgccg
     1441 ctgtggaacg ggtggggcag gacggtggtg tcgggcgcga ccaccttctg cctggagccg
     1501 tcggccgcca cctcgtcgct gacgacgaag ccgccgccca ccgagtagta cacgcggttc
     1561 tcgagctcgg cgccgtggtc gtcgtacgcg gtgaagcgca tgccgttggc gtggaacggc
     1621 agcgtctcgc ggcggtgcat cacgaggtcg gccgattcgt cgaacgcgat ctcgcggctg
     1681 ccgttgagcg tcacgcgatg ctgcgtgcgg atggcgtcga ggatggcggg cacctgctcg
     1741 atgtccaccg tgtcgggctc gtggccggcc aggcccagca gcacggcctt gtcgctgccg
     1801 tggcccttgc cggtggcccc gagcgagccg tacatctcgg ccttcacgcg cgcggtgcgc
     1861 gcgagcgcgc cctcgtgcgc gaggcgggcg acgaacatgc gcgcggcgcg catcgggccc
     1921 accgtgtgcg agctcgacgg gccgatgccg atcttgaaga ggtcgaaaac cgagactgcc
     1981 atgggatgcc ttcagctgaa acagtcggcc gcggcagcgc tcgtgacgcc ggccgcgact
     2041 tccacgatac gaccccgccg ccattcgcat ggcggcatgg gcatcaggcc ttcgcggcct
     2101 cggcttcggc ctgctcggcg aattccgaca tcggcacgca gctgcagaac aggttgcggt
     2161 cgccgtagac gttgtcgacg cggcccacgg gcgaccagta cttgcccagg cgcagcgccg
     2221 gcaccgggta ggcggcgtcc tcgcgcgagt acgcgtgggg ccactcgccc ttgagcagcg
     2281 cctgcgcggt gtgcggcgcg ttcttgagcg ggttgtcctc gcgcggccag tggccaccct
     2341 cgaccttggc gatctcggag cggatggcga tcatcgcgtc gcagaagcgg tcgagctcgg
     2401 ccagcggctc gctctcggtc ggctcgacca tcagcgtgcc ggccaccggg aacgacaggg
     2461 tcggcgcatg gaagccgtag tcgatcaggc gcttggcgac gtcttcggcg gtgatgccgg
     2521 agctgtcctt gagcgggcgc aggtcgagga tgcactcgtg cgcgacgccg ccgccctgga
     2581 tgccggcgat gttgccgctg aagtggatgt cgtagtgatg cgacaggcgc gcggcgatgt
     2641 agttggcgtt gaggatggcc gtctcggtgg cctggcgcag gccggcggcg cccatcatgc
     2701 gcacgtacat ccagctgatc ggcagcaccg cggcgttgcc cagcggggcc gcggagacgg
     2761 cgccgacgcg cttgccgtcc tcggcgccca ggccggcggc gcggtgcgcc ggcaggaagg
     2821 gcacgaggtc ggccacgacg cagaccgggc cgacgcccgg gccgccaccg ccgtgcggga
     2881 tgcagaaggt cttgtgcagg ttcaggtggc tcacgtcgcc gccgaactcg cccggcgccg
     2941 ccacgcccac catcgcgttc atgttggcgc cgtcgacgta gacgcggccg ccgtaccggt
     3001 gcaccagcgc gcacagtgcc ttgacgcccg gctcgaacac gccgtacgtg gacgggtagg
     3061 tgatcatcat cgcggccagg tcgtccttgt gc
//
LOCUS       DATCRO010000206         6061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_350727, whole genome shotgun sequence.
ACCESSION   DATCRO010000206 DATCRO010000000
VERSION     DATCRO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350727"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIN75_05515"
     CDS             complement(<1..261)
                     /locus_tag="VIN75_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEY8973657.1"
                     /translation="MSNAIFSLPHLYVLAGLVLAFVAVTTALDKRHPRRFGSAFFWGF
                     YALVFLVGDLLPPACIGAGVVAMAVIAGLHRVAPGPHDAPALA"
     gene            complement(258..995)
                     /locus_tag="VIN75_05520"
     CDS             complement(258..995)
                     /locus_tag="VIN75_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEY8973658.1"
                     /translation="MAPDVSLWPLLGVAVIIAGFVLRFNPMIVVIAAAVTTALIAKFG
                     PLGTLAAIGAGFLKTRNLTLALILPLATIGLLERHGLRQHAQAWIQRFRKATTGQLLI
                     VYLGVRELCAAVGLTGLGGHVQMVSPLIAPMAEGAAEARHGALPDKVRFRLRAMAASA
                     DNVGLFFGEDIFVAFGAVVLITTFLHEAGIDVEPLHVALWGIPTAAVAFIVHAWRLHR
                     IDAALARELGRPAADDGAAVATKEATA"
     gene            1838..1913
                     /locus_tag="VIN75_05525"
     tRNA            1838..1913
                     /locus_tag="VIN75_05525"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Asp,seq:gtc)
     gene            2009..2094
                     /locus_tag="VIN75_05530"
     tRNA            2009..2094
                     /locus_tag="VIN75_05530"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2043..2045,aa:Cys,seq:gca)
     gene            2114..2184
                     /locus_tag="VIN75_05535"
     tRNA            2114..2184
                     /locus_tag="VIN75_05535"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:His,seq:gtg)
     gene            2501..3073
                     /locus_tag="VIN75_05540"
     CDS             2501..3073
                     /locus_tag="VIN75_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8973659.1"
                     /translation="MQATHQHVLQLDIQGTPQAWISIEQAATHHASGAVAWFDGDGPL
                     RTLRGGWNVASGHQSVIEVHPIIALRGAARVNLFDRTTAVTAGKLFRRDRMTCAYCGL
                     RFTERHLQCEHIVPASRGGAWSWMNLVTACAACNGRKAARTPEEARMPLLFLPYVPSR
                     FEDFLLAGRHIRADVHEWLAARLPKGSRLS"
     gene            complement(3225..3779)
                     /locus_tag="VIN75_05545"
     CDS             complement(3225..3779)
                     /locus_tag="VIN75_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973660.1"
                     /translation="MTTDPNDISGDVSPPVTTTVANDLISEQATRRWFRTIALLAALL
                     TLGILLLTMGAIVHTFLSEFRTLALDTQVQLASAQRVAAPPAAAASAAAVAPAATHVW
                     VSLGAFTTSIAALVTALVVAATVLAIALVRASFTLTARGDDPARPIADTVEDAPVTLP
                     AADFVKAFGESVQTALKGLQAPAR"
     gene            complement(3776..4297)
                     /locus_tag="VIN75_05550"
     CDS             complement(3776..4297)
                     /locus_tag="VIN75_05550"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY8973661.1"
                     /translation="MTSPSTPPSSPAALTPARAAAALLARHWDGKLPVDPRRIAAALG
                     IRVVARGGPGDPDYAYSGYFDNSGEQPVIEYNQSEAVVRQRFTIAHELGHYALGHASS
                     PRDTPANFSTKVADDAERLANRFAAELLIPAKELRILLQTGKLKNVAEFADAFWTSQL
                     AITYRMQGLGLIP"
     gene            complement(4517..5614)
                     /locus_tag="VIN75_05555"
     CDS             complement(4517..5614)
                     /locus_tag="VIN75_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8973662.1"
                     /translation="MTAISFRQSTLPWALAHDDEERFRKLFLALMGFVIVFGIVLRFI
                     TLPEPEHAPVATLPPIAAKLLLEDKPKPPPPPPPKTSEKIEPPKPDLKDKSEPAPLPT
                     KPVPRERPVVEARQPIPGKPPGEDREAARNKAAGVGLLAALKDQPPEVHGAPLAVQLN
                     QNIDKKGAGVGTGVGVGVGAGKEQGLPDRNMITSNAAGGSGGINTAGFSRDSGGGGLA
                     GRATTLVAGVAGGGGGGGPGLGGAITGSGGHGTGAGVGKSGGTMSKSGSGKASRSLEE
                     VRLVLERAKGSLYAIYNRALREDASLQGKVVVEFTIAPNGSITSCRLVSSELKNPDLE
                     QKLLARIRTLSFAAEDVETLVTTYPLDFLPS"
     gene            complement(5697..5894)
                     /locus_tag="VIN75_05560"
     CDS             complement(5697..5894)
                     /locus_tag="VIN75_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973663.1"
                     /translation="MIPGLKAELDAISARPVAQAQNAALSHSVTIMGDKDIPYQLLRK
                     VMYTAARADFTDVSFAVNRKD"
BASE COUNT          927 a         2079 c         2183 g          872 t
ORIGIN      
        1 ggccagcgcg ggcgcatcgt gcggccccgg cgcgacgcgg tgcaacccgg cgatgacggc
       61 catcgccacg acaccggcgc cgatgcatgc gggcggcagc aggtcaccca ccaggaacac
      121 gagcgcgtag aagccccaga agaacgcgct gccgaagcgg cgcggatggc gcttgtcgag
      181 cgcggtcgtc accgccacga aggccagcac gaggccggcg agcacgtaca gatgcggcag
      241 cgagaagatc gcgttgctca tgccgtggcc tccttcgtgg cgacggccgc gccgtcatcg
      301 gccgcgggcc ggcccagctc gcgcgccagc gccgcgtcga tccgatgcag gcgccacgcg
      361 tgcacgatga acgccacggc ggccgtcgga atgccccaca gcgcgacatg cagcggctcg
      421 acgtcgatgc ccgcctcgtg caggaaggtc gtgatcagca ccaccgcgcc gaacgcgacg
      481 aagatgtcct cgccgaagaa caggcccacg ttgtcggccg atgcggccat cgcgcgcaac
      541 cggaagcgca ccttgtcggg cagcgcgccg tggcgcgcct cggctgcgcc ctcggccatc
      601 ggcgcgatca gcgggctcac catctgcacg tggccgccca ggcccgtcag gccgacggcg
      661 gcgcagagct cgcgcacgcc gaggtagacg atcagcagct ggcccgtcgt cgccttgcgg
      721 aagcgctgga tccaggcctg cgcgtgctgg cgcaggccgt ggcgctcgag caggccgatc
      781 gtggccagcg gcaggatcag cgcgagcgtg aggttgcgcg tcttgaggaa gccggcgccg
      841 atcgcagcca gcgtgcccag cggaccgaac ttcgcgatga gcgccgtcgt gaccgccgcc
      901 gcgatgacga cgatcatcgg gttgaaccgc aggacgaagc ccgcgatgat cacggccacg
      961 ccgagcagcg gccagagtga gacgtcaggt gccatgtgcg gactccagga gatgagccga
     1021 gggattgtcg ggcgagcgcg gtgcacggca ccggtcgagc gatgaggccg attctggaaa
     1081 cgcgccccgg gccgcgcaag caagaaagtt ttctttgagc tcatcgattt tgcttgtgtc
     1141 ttcgcgccgc gctgccacgc ggggcgcgcg tgggccgcgg aggcggacgt ggacaccacg
     1201 caacgaattt gtcggcgacc ccttgcgcaa tcagtaaaga ctatttacac tgcgacctgt
     1261 cgcttgaaga agcgacgatt ctttgcacga ggtgcacgcc cgagacccat gctcgacgcg
     1321 gccctcgaaa cggaaccgac ctcaggaccc catcgtgaaa agccggatcg cccttcgcaa
     1381 cgcccaagcc ctcgtcggct cgcgcgcgtt cgcctgtgcc gtcccgctgc tgacgtgcgc
     1441 accgaccttg gggcaggtgc atgcgtatcg cgactccgag gatcgctccc atccgggagg
     1501 ccgccgcgtc tgaacgacgc gccagccgct ctctgaattc cagaggcccg gattccgcga
     1561 aggatccggg cctttttgtt ttttctcttc cacttcgggt gtgcccctgc actccgacgt
     1621 cgcatcgacg cctggcgtaa gcgacgcggg atgtgcgcgg cgggaaaccc gctttcgtgc
     1681 agcgagacgc cgcctccgac gggagggctc gcgtgggggc acagccgaag tggaagtggt
     1741 caacccgaag tcgcgcaggc gctcacgccg tgcgacgcaa accggccgca acgattgggc
     1801 ggctccggaa cccgtgaccg gaacctttga ttcgcacgct tcgttaactc agcggccaga
     1861 gtgcccgcct gtctagcggg aggtcgccgg ttcaagtccg gcacgaggcg ccattcaacc
     1921 gaagtcgccg gagcgctcac gcttcgcgac gcaaaccggc cgcaacgatt gggcggctcc
     1981 ggaacccgtg accggaaact ttttcttgct cgacgtagcc aagtggtcga aggcgcctga
     2041 ttgcaattca ggtggcgcaa gcccgcgtcg gttggactcc gaccgtcgag tccagcacgt
     2101 cgtgcgctga ggggtggccg tagctcaatg gcagagccgc gggttgtggg tccgctgaca
     2161 tcggttcgat cccgttcggc cacccctgag cgcatgtcat caaacggacc ccgaggagat
     2221 ccatcatgaa acgcgaactc gaagacgacc tcagctttta cgcactccac gacgagcaag
     2281 cgccgtagcg gctacccgtc cgagacgggc gtcaagcgcg gcgaccgcgt cgtccgcggc
     2341 gacaaggagt tgagggagaa gctcggccgc aacgatccgt gcgtgtgcgg agcggcgcgc
     2401 cgcttcagga actgctgcat gaagtccggc tgcttttgac ggcagcgagc gcgcccacta
     2461 cgcgcgggac tgagaacgcc cgcgaaagga gggcaccggt atgcaagcca cgcaccagca
     2521 cgtcctgcaa ctggacatcc agggcacgcc ccaggcatgg atctcgatcg agcaggcggc
     2581 cacgcaccac gcgtcgggcg cggtggcctg gttcgacggc gacggcccgc tgcgcacgct
     2641 gcgaggcggc tggaacgtcg ccagcggcca tcagtcggtc atcgaggtgc accccatcat
     2701 cgcgctgcgc ggcgccgcgc gcgtgaacct gttcgaccgc acgactgccg tgacggccgg
     2761 caagctgttc cgccgcgacc gcatgacctg cgcgtactgc ggcctgcgct tcacggaacg
     2821 gcacctgcag tgcgagcaca tcgtgcccgc cagccgcggt ggcgcctgga gctggatgaa
     2881 cctggtcacc gcgtgcgccg cgtgcaacgg ccgcaaggcg gcgcgcacgc ccgaggaagc
     2941 gcgcatgccg ctgctgttcc tgccttacgt gccgagccgc ttcgaggact tcctgctggc
     3001 gggccggcac atccgggcgg acgtccacga gtggctggcg gcgcgcctgc ccaagggctc
     3061 gcggctgagc tgagcgtgac gtctgtgcga cgtagggcgg gttagccgaa ggcgtaaccc
     3121 gccgtttgcg tcgccggccg cggtaccggg tcgggatggc ggcgccgggc gaattggcgc
     3181 caacgaacgg cgggttacgc cttcggctaa cccgccctac gttcctaccg cgcgggcgcc
     3241 tgcaggccct tgagcgccgt ctgcaccgac tccccgaacg ccttgacgaa gtccgccgcc
     3301 ggcagcgtga ccggggcgtc ctcgaccgtg tcggcgatcg gccgcgcagg atcgtcgccg
     3361 cgcgcggtca gcgtgaagct ggcgcgcacc agcgcgatgg ccagcaccgt ggccgccacg
     3421 acaagcgccg tcaccagcgc cgcgatggag gtggtgaacg cacccagcga gacccatacg
     3481 tgcgtcgcgg ccggcgccac cgcggcggcg gaggccgcgg ccgccggcgg ggcggccacg
     3541 cgctgggcgg acgcgagctg cacctgcgtg tcgagcgcga gcgtccggaa ttccgacagg
     3601 aacgtgtgga cgatcgcccc catcgtcagc aacaggatgc ccagcgtcag cagcgccgcg
     3661 agcagggcga tggtgcggaa ccagcggcgg gtggcctgct cggagatcag gtcgttggcg
     3721 accgtcgtcg tgaccggggg cgacacgtcg ccgctgatgt cgttcgggtc ggtcgtcatg
     3781 gaatcagtcc gagcccctgc atgcgatagg tgatggccaa ttgcgaggtc cagaacgcgt
     3841 cggcgaactc ggccacgttc ttgagctttc ccgtctgcag caggatgcgc agttccttgg
     3901 cgggaatcag cagctccgcg gcgaaccggt tggcgaggcg ctcggcgtcg tcggccacct
     3961 tggtgctgaa gttggccggg gtgtcgcgcg gcgagctcgc atggcccagc gcgtagtggc
     4021 cgagctcgtg cgcgatggtg aagcgctggc gcaccaccgc ttcggactgg ttgtactcga
     4081 tgaccggttg ctcgccgctg ttgtcgaagt agccgctgta cgcgtagtcc gggtcgcccg
     4141 ggccgccgcg cgcgaccacg cggatgccca gcgccgcggc gatccgccgc gggtcgacgg
     4201 ggagcttgcc gtcccagtgc ctggccagca gggccgcggc cgcgcgcgcg ggcgtgagcg
     4261 ccgcgggcga ggagggtggg gtggacgggg aggtcatggg cggggcggag ttgtcgcgtt
     4321 ggggtgctcg gcgagcacct cgatccacgc attttgccgc caggagccca ttttcaagca
     4381 tcccgagggg gaccgccccg cgccgatcgc gcgataacgg gccagccggc gctgcggcgc
     4441 ccgcgcgtgc ccatgaaaaa cgccggccgc cgcgatcgga tcgcgagggc cggcgtggcg
     4501 cgcagggcgc gtgggatcag gacggcagga agtccagcgg gtaggtggtg acgagcgtct
     4561 ccacgtcctc cgcggcgaac gacagcgtgc ggatccgcgc gagcagcttc tgctcgagat
     4621 ccgggttctt cagctcgctg gacaccagcc ggcacgacgt gatgctgccg ttgggggcga
     4681 tggtgaactc gacgacgacc ttgccctgca gcgaggcgtc ctcgcgcagc gcgcggttgt
     4741 agatcgcgta cagcgagccc ttggcgcgtt caagcaccag gcgcacctcc tccagcgagc
     4801 gcgacgcctt gccgctgccg ctcttggaca tcgtgccgcc ggacttgccg acgcccgcgc
     4861 ccgtgccatg gccgccggag ccggtgatgg cgccgcccag gcccgggccg ccgcctccgc
     4921 cgccaccggc cacgcccgcg acgagcgtgg tggcgcggcc cgccaggccg ccgccgcccg
     4981 aatcgcggct gaagcccgcg gtgttgatcc cgcccgagcc gccggcagcg ttggacgtga
     5041 tcatgttgcg atcgggcagg ccctgctcct tgccggcgcc caccccgaca ccgacgccgg
     5101 tgcccacgcc ggcgcccttc ttgtcgatgt tctggttgag ctgcacggcc agcggggcgc
     5161 catgcacctc gggcggctgg tccttgagcg cggccagcag gcccacgccc gcggccttgt
     5221 tgcgcgcggc ctcgcgatcc tcgcccggcg gcttgcccgg gatcggctgg cgcgcctcca
     5281 ccacgggccg ctcgcgcggc acgggcttgg tgggcagcgg cgccggctcg gacttgtcct
     5341 tgaggtcggg cttgggcggc tcgatcttct ccgacgtctt gggcggcgga ggcggcggcg
     5401 gcttcggctt gtcttccagc aggagcttgg cggcgatcgg cggcagcgtg gccaccggcg
     5461 catgctcggg ctcgggcagc gtgatgaagc gcagcacgat gccgaacacg atcacgaagc
     5521 ccatcaacgc gaggaacagc ttgcggaagc gctcctcgtc gtcgtgcgcc agcgcccacg
     5581 gcagcgtcga ttggcggaag gagatcgcgg tcatggtgca gcgcagcgct cggccgccct
     5641 gagcaagctg ccgccccctc gggcggcagc gaacagagtg agcgtggggg ccatcgtcag
     5701 tccttcctgt tcacggcgaa ggagacgtcc gtgaagtcgg cgcgcgcggc ggtgtacatc
     5761 accttgcgca gcagctggta cgggatgtcc ttgtcgccca tgatcgtcac cgagtgcgac
     5821 agcgccgcgt tctgcgcctg ggccacggga cgcgcggaga tcgcgtcgag ctcggccttc
     5881 aggcccggga tcaagtcgtc ggtggcggcg gcgggggctt gggcttgtct tccagcagga
     5941 gcttggcggc gatcggcggc agggtcgctg ccggcgcgcg cgcgggctcg ggcaacgtga
     6001 tgaagcgcag cgcgaggccg aacacgatca cgaagcccat cagggcgagg aacagcttgc
     6061 g
//
LOCUS       DATCRO010000207         9147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_812484, whole genome shotgun sequence.
ACCESSION   DATCRO010000207 DATCRO010000000
VERSION     DATCRO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812484"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VIN75_05565"
     CDS             complement(<1..873)
                     /locus_tag="VIN75_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8973664.1"
                     /translation="MTDVLDTTAAPRTFRAACPHDCPDTCAMRVTVEGDKVIKLHGDP
                     DHPTTNGALCTKVSRYAERTEHDERVLKPLKRVGPKGAGQFVEVSWDEALDDIAARLA
                     EIARRDPEAIVPYSYAGTMGQVQGEGMAARFFNKLGASRLDRTICSNAGGDALLATYG
                     GKVGMHVEHFAESKLIVIWGSNSITSNLHFWPIALAAKRAGATLVCIDPRRTETAEKC
                     HVHLALTPGSDGALALAVMHELIVNDWLDHDYIARHVEGWDGLRERALQWTPERAAPE
                     CGLTVDQIRDFARLY"
     gene            1218..2447
                     /locus_tag="VIN75_05570"
     CDS             1218..2447
                     /locus_tag="VIN75_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY8973665.1"
                     /translation="MNVIESLLADAAAITALRREIHSYPELCFKEDRTSDLVAAKLTE
                     WGIPIRRGLGKTSVVGIIEGKRGKNGGAIGLRADMDALPMAESNTFAHASRNPGRMHA
                     CGHDGHTAMLLAAAQHLARHRDFDGIVYLLFQPAEEGGGGAREMIKDGLFTEFPMDAV
                     FGMHNWPGIKAGQLAVCPGPCMASSNEFFITIRGRGSHAALPHLSIDPVPIACQMVQA
                     FQTIVTRNKKPTDTGVISVTTIHTGEATNVVPDSAHIEGTVRTFTLPVLDLIEQRMRE
                     IARHTAAAFGATVEFRFHRNYPPTVNHAAEAEFVAGVMRDTVGAENTLAFEPSMTAED
                     FSYFLLEKPGAYFVIGNGDGRHRLAYSGDATDTSDSHNLGPCTLHNPHYDFNDDLIPL
                     GGTLWIRLVEKWFAQRD"
     gene            2492..3577
                     /locus_tag="VIN75_05575"
     CDS             2492..3577
                     /locus_tag="VIN75_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8973666.1"
                     /translation="MRTPPEPFAHNYAEARAKFLAAANGTQLESHPEPLRGLELETLA
                     MDVARIGADDADALLVVSSGCHGVEGYCGSGAQVAMLHDEALRNKARLAGVAMLFIHA
                     LNPWGFSFGRRVTHEGVDLNRNFIDFRRPPPGNPGYDQLAAFLLPEDWPPTVDDERQI
                     LAWGGSHGMPALQAAISGGQYRHPDGLFYGGLSPTWSHQTLRMVLRQHATRCKLLGWI
                     DLHSGLGESGVGERIFAARDEDGAKHRAKAWWGNRITFTDDGSSTSSDLHGNLWHAVY
                     DECPQAAYAGITLEFGTQPAEAVLRALRYDHWFARRRKLHDERAEFARAAMRQAFFTD
                     TPEWKQAVITQSRAAAAQAIDGMAAWR"
     gene            3705..4262
                     /locus_tag="VIN75_05580"
     CDS             3705..4262
                     /locus_tag="VIN75_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8973667.1"
                     /translation="MQTALIMIDVQESFRQRPYWSQSGADVFLVHANALIDGAQARGI
                     PIVRVFHTDGPPTPDNPFAHGGPFVRPLTGLADFEAALTIEKHRHSSLVGTPLATWLV
                     RRGIRRLIIAGIRTEQCCETTTRHASDEGWEVDFCGDATLTFDMKTPSGRTLTAAEIR
                     ERTETVLVDRFATLVSVDQALARAH"
     gene            4270..5259
                     /locus_tag="VIN75_05585"
     CDS             4270..5259
                     /locus_tag="VIN75_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8973668.1"
                     /translation="MIDVLFLVLPETLLLDLVGPAEALRLANQQLAARGQPPAFRLRY
                     VCAQPEVTTSIGLQLSGLEPLPDGLPPGAWVVLLGRPGLKDTLPTMQRNGWLGARHWL
                     STVVAPALARVTPAGATPHRLLTVCVGTLLAADAGLVGRRQVTTHHEHLEALAALAPQ
                     AQVCANRVFVVDGPLWSSAGITAGIDLALHCIGSVCGDAVAAAVAETMVVFQRRGADD
                     PQRSPLLDGRNHLHPAVHRVQNAVCERPGHDWDVPALAAVAHVTPRHLARLFKDHAGI
                     SPRDYVEHVRSGLAEQAIARGLPTAQAIELAGFGTDRQFRRARARRRAQDVSA"
     gene            complement(5256..5639)
                     /locus_tag="VIN75_05590"
     CDS             complement(5256..5639)
                     /locus_tag="VIN75_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973669.1"
                     /translation="MSKILVVDDLRDSADSLALLFDAMGHQTLTSYDGQEAVEAAQSF
                     EPDIVFLDINMPILDGYGAARSLRDARVDAPPVLVALTAVSGPDAKRKADDAGFDFYV
                     TKPADFNVLITIVDDLSRRMANRPH"
     gene            complement(5828..6463)
                     /locus_tag="VIN75_05595"
     CDS             complement(5828..6463)
                     /locus_tag="VIN75_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8973670.1"
                     /translation="MTSRSKPDRALVFDFGRVLFRWRPDALLAQVLPHRASDPESTRH
                     WVGQVFQAYGGDWGEFDRGTVEPEELARRIAARTGLTEDEVAAVIAACPGELEPLPDT
                     VAWLRRLHARGVPLHYLSNMPEPFAQHFERTHDFMRLFESGVFSSRVQLIKPDPAIFV
                     HAAEVFGRAPANLLFLDDHEPNIVAARAAGWQAVHFLDAAQAEAQVRALGW"
     gene            complement(6619..8247)
                     /gene="pgi"
                     /locus_tag="VIN75_05600"
     CDS             complement(6619..8247)
                     /gene="pgi"
                     /locus_tag="VIN75_05600"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8973671.1"
                     /translation="MTFVRCDQTEAWKALQAHYDASGRQFDLRTAFASDAGRFDHFAI
                     EAPGVFADLSKNRWDAGTRALLTGLAAECGLEARRDAMFAGEPINLTEGRAVLHTALR
                     APSNAAPISAVTDAAIGEVHSVLDPLLAYAERVRAQAGTQFTDVLNIGIGGSDLGPQM
                     AVIALQEFVHPQLRFHFVSNVDGHDIAPVLARLDPSKTLVIVASKTFTTQETLANANV
                     ARDWFTANGGTDVATHFVGVTTNIEAAAKFGITTTFGFWDWVGGRYSLWSAIGLSIAI
                     AVGADGFKEFLAGAHAMDEHFRSAPLARNLPVQLALIDVWYRNFHGFTSRSVAPYHQG
                     LKRFPAYLQQLEMESNGKRVDVEGRAVPFATSPVVWGEPGTNGQHAYFQMLHQGTDVV
                     PVEFIAVKRPAAGHDGAYADLHVKLLANCLAQSQALMQGKAPAEAAQESPPTASRTIA
                     REVIAEHRGFPGNRPSTTLVLDALTPRTLGALVALYEHRVFCSGALWGINSFDQWGVE
                     LGKALCNALLPRLASGATDGLDSSTAGLLTRLKK"
     gene            complement(8318..>9147)
                     /locus_tag="VIN75_05605"
     CDS             complement(8318..>9147)
                     /locus_tag="VIN75_05605"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY8973672.1"
                     /translation="VQQADYAPLLADTVAAHQGKPLADVVDQVLVRFGLEILKVVPGR
                     VSTEVDARLSFDTAATVARARGIIKLYEAAGIGRERVLIKVASTWEGIAAARQLEAEG
                     IHCNLTLLFSFCQAVACGDGGITLISPFVGRIYDWYKKQAGAKWDEAAQAGVNDPGVQ
                     SVTRIFNYYKKFGIRTEVMGASFRNVGQIQALAGCDLLTISPDLLGSLQSDTGALPKV
                     LDAEAAQALQLERVHFDEPAFRFALNEDAMATEKLAEGIRAFAADSIKLDALIEAAR"
BASE COUNT         1336 a         3283 c         3214 g         1314 t
ORIGIN      
        1 gtacaggcgc gcgaaatcgc ggatctggtc gaccgtgagg ccgcactccg gcgccgcccg
       61 ctcgggcgtc cactgcagcg cgcgttcgcg caggccgtcc cacccctcga catgccgggc
      121 gatgtagtcg tgatcgagcc agtcgttgac gatcagctcg tgcatcaccg ccagcgccag
      181 cgcgccgtcg ctgccggggg tgagcgcgag gtggacgtgg cacttctcgg ccgtctcggt
      241 gcgacgcggg tcgatgcaca cgagcgtggc accggcgcgc ttggcggcca gcgcgatcgg
      301 ccagaagtgc aggttcgacg tgatcgagtt gctgccccag atgacgatga gcttgctctc
      361 ggcgaagtgc tcgacgtgca tgcccacctt gccgccgtag gtggccagca gcgcgtcgcc
      421 gccggcgttg gagcagatgg tgcgatccag ccgcgacgcg cccagcttgt tgaagaagcg
      481 cgcggccatg ccctcgccct gcacctggcc catcgtgccg gcgtagctgt agggcacgat
      541 ggcctcgggg tcgcggcggg cgatctctgc cagccgggcg gcgatgtcgt ccagcgcctc
      601 gtcccacgac acctcgacga actgacccgc gcccttgggc ccgacgcgct tgagcggctt
      661 gagcacgcgc tcgtcgtgct cggtgcgctc ggcgtagcgc gacaccttcg tgcacagcgc
      721 gccgttggtg gtcggatggt cggggtcgcc gtgcagcttg atgaccttgt cgccctcgac
      781 ggtgacgcgc atcgcgcagg tgtcggggca gtcgtgcggg caggcggcgc ggaaggtgcg
      841 cggcgcggcg gtcgtgtcga ggacgtcggt catgccgaaa tgatcccaca acgctcctgc
      901 cgagcgagcg gcaaggccac tccggcaggg gctgtttccc gcagcgcgac aggcttaaga
      961 tgggcttcat acccgatccc gcgggcgacg accggagggg tctggcctct cctcacgtcg
     1021 cgcgcagtcg tcgagcgcag gaaagccatc gccacgacgt gaggacaggc ctgaccccga
     1081 tccccggctg acgatcgggg tcaggcctgt cctcgaatcg cggtcgcttc acggtctccg
     1141 gcaagaatcc tggcgcgatc cgaggagagg ccagagccct cgggccggac gcctcgcgcc
     1201 ttcacgactc catcaccatg aacgtcatcg aatccctgct ggccgacgcg gccgccatca
     1261 ccgcgctccg acgagagatc cactcgtatc ccgagctctg cttcaaggag gatcgcacca
     1321 gcgacctcgt ggccgccaag ctcaccgagt ggggcatccc catccggcgc ggcctgggca
     1381 agaccagcgt cgtcggcatc atcgagggca agcgcggcaa gaacggcggc gccatcggcc
     1441 tgcgcgccga catggacgcc ctgcccatgg ccgagtccaa cacctttgcc cacgcgagcc
     1501 gcaacccggg ccgcatgcac gcctgcggcc acgacggcca caccgcgatg ctgctggccg
     1561 ccgcgcagca cctcgccagg caccgggact tcgacggcat cgtctacctg ctgttccagc
     1621 cggccgagga aggcggcggc ggcgcgcgcg agatgatcaa ggatgggctg ttcaccgagt
     1681 tcccgatgga cgccgtgttc ggcatgcaca actggcccgg catcaaggcc ggccagctgg
     1741 ccgtctgccc ggggccgtgc atggcgtcga gcaacgagtt cttcatcacg atccgcggcc
     1801 gcgggtcgca cgccgcgctg ccgcacctgt ccatcgaccc ggtgcccatc gcctgccaga
     1861 tggtgcaggc gttccagacc atcgtcacgc gcaacaagaa gcccaccgac acgggtgtca
     1921 tctcggtcac cacgatccac accggcgagg ccaccaatgt cgtgccggac tcggcgcaca
     1981 tcgagggcac ggtgcgcacg ttcacgctgc cggtgctcga cctgatcgag cagcgcatgc
     2041 gcgagatcgc ccggcacacg gccgccgcct tcggcgccac ggtggagttc cgcttccacc
     2101 gcaactaccc gcccaccgtc aaccatgcgg ccgaagccga gttcgtcgcc ggcgtcatgc
     2161 gcgacaccgt cggcgccgag aacacgctgg ccttcgagcc gtcgatgacc gccgaggact
     2221 tcagctactt cctgctcgag aagccgggcg cctacttcgt catcggcaac ggggacggcc
     2281 gccaccgcct cgcctacagc ggtgacgcga ctgacacatc ggattcgcac aatctcgggc
     2341 cgtgcacgct gcacaacccg cactacgact tcaacgacga cctgatcccg ctgggcggca
     2401 cgctctggat ccggctcgtc gagaaatggt tcgcgcaacg cgactgaccc ggccggcgcg
     2461 acgccggcca tcctgagcca gggagacgag catgaggact ccgccggaac cgtttgccca
     2521 caactatgcc gaggcgcgcg cgaaattcct cgcggcggcc aacggcacgc agctggagtc
     2581 gcatcccgag ccgctgcgcg gactggagct cgagacgctc gcgatggacg tcgcgcgcat
     2641 cggcgccgac gacgccgacg cgctgctggt cgtctccagc ggctgccacg gggtcgaggg
     2701 ctactgcggc tccggcgcgc aggtggccat gctccacgac gaggctctgc gcaacaaggc
     2761 gcggctggcg ggcgtggcca tgctgttcat ccacgcgctc aatccgtggg gcttctcgtt
     2821 cgggcgccgc gtcacgcacg agggcgtcga cctcaaccgc aacttcatcg atttccgccg
     2881 cccgcccccc ggcaatccgg gctacgacca gctggccgcg ttcctgctgc ccgaggactg
     2941 gccgccgacg gtcgacgacg agcgccagat cctggcctgg ggcggctcgc acggcatgcc
     3001 ggcgctgcag gcggcgatct ccggcggcca gtaccgccac cccgacggcc tgttctacgg
     3061 cggcctgagc cccacctgga gccaccagac gctgcgcatg gtgctgcgcc agcacgccac
     3121 gcgctgcaag ctgctgggct ggatcgacct gcacagcgga ctcggcgaga gcggcgtggg
     3181 cgagcgcatc ttcgccgccc gcgacgagga cggcgccaag caccgcgcga aggcctggtg
     3241 gggcaaccgc atcaccttca ccgacgacgg atcgtcgacg tcgtccgacc tgcacggcaa
     3301 cctgtggcac gccgtttacg acgagtgccc gcaggcggcc tacgccggca tcacgctgga
     3361 gttcggcacg cagcccgccg aggccgtgct gcgcgcgctc cgctacgacc actggttcgc
     3421 gcgccggcgc aagctgcacg acgagcgcgc cgagttcgcg cgcgccgcga tgcgccaggc
     3481 gttcttcacc gacacgcccg agtggaagca ggccgtgatc acgcagtcgc gcgccgccgc
     3541 ggcccaggcg atcgacggga tggcagcctg gcgttagggc ctgtccacgc acgactcacg
     3601 ccagcaccga tgtcctgaaa cgtccgcaag ctgtcgcgcg gcggccctgc cagaatggcg
     3661 gccggagggc aacattcctg tcatccaacc acaggagaga agccatgcag accgccctga
     3721 tcatgatcga cgtccaggag tccttccggc agcgcccgta ctggtcgcaa tctggcgcgg
     3781 acgtgttcct ggtgcacgcc aacgcgctga tcgacggcgc ccaggcgcgc ggcatcccga
     3841 tcgtgcgcgt gttccacacc gacggccccc cgaccccgga caacccgttc gcgcacggcg
     3901 gcccgttcgt gcgcccgctc accggcctgg ccgacttcga ggccgcgctc acgatcgaga
     3961 agcatcgcca cagctcgctg gtgggcacgc cgctggcgac ctggctggtg cgccgcggca
     4021 tccgacgcct gatcatcgcg ggcatccgca ccgagcagtg ctgcgagacg accacgcgcc
     4081 acgccagcga cgagggctgg gaggtcgact tctgcggcga cgcgacgctc accttcgaca
     4141 tgaagacgcc atcgggccgc acgctcacgg ccgccgagat ccgcgagcgc accgagaccg
     4201 tgctggtcga ccgcttcgcg acgctggtat cggtggatca ggcgctggcc cgcgcgcact
     4261 gacgccgcca tgatcgacgt cctgttcctg gtgctgcccg agacgctgct gctggatctc
     4321 gtcggacccg cggaggcgct gcgcctggcc aaccagcagc tggccgcgcg cggccagccg
     4381 ccggccttcc ggctgcgcta cgtctgcgcg cagccggagg tgacgacgtc gatcggcttg
     4441 cagttgtccg gcctggagcc cctgccggac ggcctgccgc ccggggcctg ggtcgtgctg
     4501 ctggggcggc ccggtctgaa ggacacactg cccacgatgc agcgcaacgg ctggctcggc
     4561 gcccgccatt ggctgtcgac ggtggtcgcg cccgcgctcg cgcgcgtcac gcccgcgggg
     4621 gcaaccccgc accggctgct gaccgtgtgc gtcggcaccc tgctggcggc cgacgccggg
     4681 ctggtcggac gccgccaggt cacgacgcac cacgagcacc tggaggcgct ggccgcgctg
     4741 gcgccgcagg cccaggtgtg cgcgaaccgc gtcttcgtcg tcgacgggcc gctgtggtcc
     4801 agcgccggca tcaccgccgg catcgacctc gcgctgcact gcatcggcag cgtctgcggc
     4861 gacgcggtgg ccgcggccgt cgccgagacg atggtcgtgt tccagcgccg cggcgccgac
     4921 gacccgcagc gctcgccact gctggacggc cgcaaccacc tgcacccggc cgtgcaccgg
     4981 gtgcagaacg cggtgtgcga gcggcccggc cacgactggg acgtgccggc gctggccgcc
     5041 gtggcccacg tgacgccgcg acacctggcg cgactgttca aggatcacgc cggcatctcg
     5101 ccgcgcgact acgtcgagca cgtgcgcagc ggcctggccg agcaggcgat cgcgcgcggg
     5161 ctgccgaccg cgcaggcgat cgaactcgcc ggcttcggca ccgaccgcca gttccgccgc
     5221 gcccgcgccc ggcggcgcgc gcaagacgtc tccgcctagt gcggccggtt ggccatccgc
     5281 cgcgacaggt cgtcgacgat ggtgatcagc acgttgaagt ccgccggctt ggtgacgtag
     5341 aagtcgaagc cggcgtcgtc ggccttgcgc ttggcgtccg gcccggagac ggcggtcagc
     5401 gccaccagca cgggcggcgc gtcgaccctc gcgtcgcgca gcgagcgcgc cgcgccgtag
     5461 ccgtcgagga tgggcatgtt gatgtccagg aacacgatgt ccggctcgaa cgactgcgcc
     5521 gcctccaccg cctcctggcc gtcgtaggac gtcagcgtct ggtggcccat cgcatcgaac
     5581 agcagggcca ggctgtcggc actgtcgcgg aggtcgtcca cgacgaggat cttggacatc
     5641 ggataggttc cccggcggat tgactggccg ccttcatcga cgcgggctgc ggcgcgtccg
     5701 ggctcccggc ggcaacgctg ggcgcatgcc gccaccggac gcggtcaccg cgtaccgcct
     5761 aggttacttg ttttggccga ggcctgcccg attgaatggg cggcgtgcac tttgcgcgac
     5821 agtggcgtca ccagccgagc gcgcgcacct gggcctcggc ctgggcggca tcgaggaagt
     5881 gcaccgcctg ccagcccgcg gcccgggccg ccacgatgtt gggctcgtgg tcgtcgagga
     5941 acagcaggtt cgccggggcg cggccgaaca cctcggccgc atgcacgaag atcgccgggt
     6001 cgggcttgat cagttgcacg cgcgacgaga acacgccgga ctcgaacagc cgcatgaagt
     6061 cgtgcgtgcg ctcgaagtgc tgtgcgaagg gttcgggcat gttcgacaag tagtgcagcg
     6121 gcacgccgcg cgcgtgcagc cggcgcagcc acgccaccgt gtcgggcagc ggctcgagct
     6181 cgccggggca ggccgcgatg acggccgcga cctcgtcctc ggtcaggccc gtgcgcgcgg
     6241 cgatgcggcg cgcgagctcc tcgggctcga ccgtgccgcg atcgaactcg ccccagtcgc
     6301 cgccataggc ctggaacacc tggccgaccc agtgcctggt gctctcgggg tcggaggcgc
     6361 ggtgcggcag cacctgggcc agcagcgcgt cggggcgcca gcggaacagc acgcggccga
     6421 agtcgaagac gagggcgcgg tcgggcttgg agcgagaagt catcgatcga attgaaggaa
     6481 gcggcccgaa tccagccgcc tccgcatttc aacaccgctc gaacaacccg gctgccgccc
     6541 tggccggcga gcgctcggcc gctccggagc gagccggcct gagccccttg ggggcggcga
     6601 gcggagcgag ccgggctgtc atttcttcaa gcgggtcagc agtccggccg tcgacgaatc
     6661 gagcccgtcc gtggcgccgc tggccagtcg cggcagcagc gcgttgcaca gcgccttgcc
     6721 gagttccacg ccccactggt cgaagctgtt gatgccccac agggcgccgc tgcagaacac
     6781 gcggtgctcg tacaaggcga ccagcgcgcc gagcgtgcgc ggcgtgagcg cgtccagcac
     6841 cagcgtcgtg ctggggcggt tgccggggaa gccgcggtgc tcggcgatga cctcgcgcgc
     6901 gatcgtgcgc gaggccgtcg gcggcgattc ctgcgcggcc tcggccggcg ccttgccctg
     6961 catcagcgct tgcgactgtg cgaggcagtt ggccagcagc ttcacgtgca ggtcggcgta
     7021 ggcaccgtcg tggcccgcgg ccggcctctt caccgcgatg aattccaccg gcacgacgtc
     7081 ggtgccctgg tgcagcatct ggaagtacgc gtgctgcccg ttggtgccgg gttcgcccca
     7141 cacgaccggg ctggtggcga acggcacggc gcggccctcg acatcgacgc gcttgccgtt
     7201 ggactccatc tccagctgct gcaggtaggc cgggaagcgc ttgaggccct ggtggtacgg
     7261 cgcgacgctg cggctcgtga agccgtggaa gttgcggtac cagacgtcga tcagcgcaag
     7321 ctgcaccggc aggttgcgcg cgagcggcgc gctgcggaag tgctcgtcca tcgcgtgcgc
     7381 gccggccagg aactccttga agccgtcggc gccgacggcg atcgcgatcg acaggccgat
     7441 cgcgctccac agcgagtagc ggccgccgac ccagtcccag aagccgaagg tggtggtgat
     7501 gccgaacttc gccgcggcct cgatgttggt cgtgacgccc acgaagtgcg tcgcgacgtc
     7561 ggtgccgccg ttggccgtga accagtcgcg cgcgacgttg gcgttggcca gcgtctcctg
     7621 cgtcgtgaac gtcttggacg cgacgatcac cagcgtcttg ctcggatcca gccgcgcgag
     7681 caccggcgcg atgtcgtggc cgtcgacgtt ggagacgaag tggaagcgca gctgcggatg
     7741 cacgaactcc tgcagcgcga tcacggccat ctgcgggccc aggtcggagc cgccgatgcc
     7801 gatgttgagc acgtccgtga actgcgtccc ggcctgcgcg cgcacgcgtt ccgcgtaggc
     7861 gagcagcggg tcgagcaccg aatgcacctc gccgatggcg gcgtcggtga ccgcgctgat
     7921 gggcgccgcg ttcgacggcg cgcgcagcgc ggtgtgcagg acggcgcggc cttcggtcag
     7981 gttgatcggc tcgccggcga acatcgcgtc gcgccgcgcc tcgaggccgc actcggcggc
     8041 caggcccgtc agcagcgcgc gggtgccggc gtcccagcgg ttcttggaca ggtcggcgaa
     8101 gacgccgggc gcctcgatgg cgaaatggtc gaagcggccg gcatcggacg cgaacgccgt
     8161 gcgcaggtcg aactgcctgc cgctcgcgtc gtagtgcgcc tgcagcgcct tccaggcctc
     8221 ggtctggtcg cagcgcacga aagtcatgct tgcccccttg ccgctgcgcg gcgtcccccc
     8281 gagggggagc attcgccctt ggggcggcgc tgcgggctca tcgcgcggcc tcgatcagcg
     8341 cgtcgagctt gatcgagtcg gcggcgaagg cgcgaatgcc ttcggcgagc ttctcggtgg
     8401 ccatcgcgtc ctcgttgagc gcgaagcgga acgcgggctc gtcgaagtgc acgcgctcca
     8461 gctgcagcgc ctgcgcggcc tcggcgtcga gcaccttggg cagcgcgccg gtgtccgatt
     8521 gcagcgagcc cagcaggtcg gggctgatgg tgagcaggtc gcagcccgcc agcgcctgga
     8581 tctggccgac gttgcggaag ctcgcgccca tcacctcggt cctgatgccg aacttcttgt
     8641 agtagttgaa gatgcgggtg accgactgca cgcccgggtc gttgacgccg gcctgcgcgg
     8701 cctcgtccca cttcgcgccg gcctgcttct tgtaccagtc gtagatgcgg ccgacgaacg
     8761 gcgagatcag cgtgatgccg ccgtcgccgc aagccaccgc ctggcagaag ctgaacagca
     8821 gcgtgaggtt gcagtggatg ccctcggcct cgagctggcg cgcggcggcg atgccttccc
     8881 aggtcgacgc gaccttgatc agcacgcgct cgcggccgat gcccgcggct tcgtagagct
     8941 tgatgatacc gcgcgcgcgg gccacggtgg ccgcggtgtc gaacgacagc cgcgcgtcga
     9001 cctcggtgga gacgcggccc ggcacgacct tcaggatctc gaggccgaag cgcaccagca
     9061 cctggtcgac cacgtcggcc agcggtttgc cctggtgggc ggcgacggtg tcggccagca
     9121 agggggcgta gtcggcctgc tgcacgg
//
LOCUS       DATCRO010000208        13020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_215784, whole genome shotgun sequence.
ACCESSION   DATCRO010000208 DATCRO010000000
VERSION     DATCRO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215784"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1503
                     /locus_tag="VIN75_05610"
     CDS             172..1503
                     /locus_tag="VIN75_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973673.1"
                     /translation="MNSSVLNVATAAAVVLAASGPQAAHAAVDPVKAQALFDEAARLC
                     QADGGALWHHSLCGPILLVDWTDNTAVANQPDPAGVLEPVGKVFAGTLPADVVIANTP
                     IEWSGKRWTELLWPLPDDVAHRHVTLSHELFHRAQHELQMPMGDGGNLHLDTLEGRTL
                     LQLEWHALARALVAPDAVARNAAVSDALLFRHERYRLFPDARAEEHALELNEGVAEYT
                     GVRVGLPTARERTAYALRDLECFIQSPTFVRSFAYATGPAWGLLLDKADPAWRDELAA
                     AMKAGHPQGLDDMLQAALKLPEPSPATVKAREADYDDTLRPREQARDQARQAHLAELK
                     ARLVDGPVLRLPLAHLHASYQFNPQTLEALGSDGVVYPTMTLSADWGTLKVEQGALLD
                     KAMSGAAVSAAGSSADHLQGPGWRLTLAKGWTVAAGERNGDFVVKQESDSH"
     gene            1612..2079
                     /locus_tag="VIN75_05615"
     CDS             1612..2079
                     /locus_tag="VIN75_05615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8973674.1"
                     /translation="MSEAPNTLAWDAPRPHVLPVVVNDGHIDLMQHVNNVHYLQWLEN
                     VAWDHSVALGMRPEDYTRLGQGMVVRRHELDYVAPALLGDRVLLATWIVGLDRLSLHR
                     RYQFVRESDGVTLFRGATHFVCVEIASGRVRRMPPDFTEVYARGFMPWPSPGA"
     gene            complement(2051..2494)
                     /locus_tag="VIN75_05620"
     CDS             complement(2051..2494)
                     /locus_tag="VIN75_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8973675.1"
                     /translation="MNDADQPIRAALAAYQAAVLAKDVDAFVSVYSEDAQIFELWGTW
                     VHDIGSWREMAKGWFAFLGDQRSVVEAHGVRTQVSGDMAMLTASLTYRAVDPAGQELR
                     SLDNRLTWVLRQRGGRWQVVHEHTSVPLAHEDGKGIVKRPATATA"
     gene            complement(3002..3811)
                     /locus_tag="VIN75_05625"
     CDS             complement(3002..3811)
                     /locus_tag="VIN75_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY8973676.1"
                     /translation="MNPNVPFRKGRMTPFQSRRRWLTTLAAGAGTLALGGCDRLVEDP
                     RIAGTLAGTEKLTLSSQRLLLDHQPLAREYGERDLSPVFRANGSSMPSSPEYARLLQG
                     RFADWRLQVDGLVQRPLSLSLAELKAMKARSQITRHDCVEGWSAIGKWQGVPLSQVLD
                     LAGLKPGARYVIFHCADNLAGTTDDSGLYYESIDLLDAYHPQTILAYGMNGQDLPVAH
                     GAPLRLRVERQLGYKQAKYVMRVEVADAYAHLNGGKGGFWEDRGYEWYAGI"
     gene            complement(3808..4698)
                     /locus_tag="VIN75_05630"
     CDS             complement(3808..4698)
                     /locus_tag="VIN75_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8973677.1"
                     /translation="MTSPPAMTTARMPSGMFRQRAARPARQLVPRHSVLVRLTHWINV
                     LCFSLLLMSGAQIFNAHPRLYWGEYGADHDHAWLSMDSLRSNGHWRGVLHVGSLTVET
                     TGVLGASRDAAGNLQPRGFPSWLTIPSYQDLADGRHWHFFFAWLFAINGAVYLVGGLL
                     SRHFRRDLLPTRAELAPSHLAREVVDHAKLDFPKGEAARHYNVLQKLSYLAVAFVLLP
                     GMVLTGLTMSPGLDSAFPFLLDLFGGRPSARSLHFIFASALVAFVVVHLVMVLVSGVW
                     NNLRSMITGRYAIETKEDAR"
     gene            complement(4836..5831)
                     /locus_tag="VIN75_05635"
     CDS             complement(4836..5831)
                     /locus_tag="VIN75_05635"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8973678.1"
                     /translation="MKVCIYGAGAIGLLVGARLAATGQHQVSAVARGATLAALNEHGL
                     RVDGFAGPISGPVAASADPGALGVQDVVVIAVKAPALADVGRHIAPLLEPRTLIVPAM
                     NGVPWWFCHGLSGPAAGLALKSVDPDGSIARHLPLEQVIGCVVHAAASVVEPGKAHNQ
                     MGRGLVIGEPRGGVSGRVAALKAALDAAGFECTASEMIQRDIWYKLWGNMTTNPISAI
                     TGATTDRIIGDELVRKLTSGAMLESGRIGERLGFAITQSPEERHAITRKLGAFKTSML
                     QDVEAGRAIELDSLVGAVRELGAQVGVETPLIDAIFGLARVFGQAHGLYPESPAA"
     gene            complement(6005..6577)
                     /locus_tag="VIN75_05640"
     CDS             complement(6005..6577)
                     /locus_tag="VIN75_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY8973679.1"
                     /translation="MSSGFHVHGPHDHEVEHAIEHGARGMTGQLAVITAVLATVGALF
                     SYMGGATQADAALAKNDAAIRKTEASDQWNFYQAKNARQAIAEVALDIVPAERRDAYA
                     KNIEKYKSDKEPIKAKAEALEAEAKDFDDRSEHLMHQHHRWAEATTAIQVAIALAAIA
                     LLTRRRWLEWCTLGVAGVGIAIGIAAFLKV"
     gene            6768..7574
                     /locus_tag="VIN75_05645"
     CDS             6768..7574
                     /locus_tag="VIN75_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY8973680.1"
                     /translation="MKPELATRFHHLHADGLLLLANAWDAGSARLAQAAGARAVATSS
                     AAVAWAHGWPDGDALPLELVLQTTRAVAAAVDVPVTVDIEGGYSDDPARVGELVAQVV
                     AAGAVGINIEDGSGDPALLAAKIAAARQAATALGVDLFINARTDVWLRGLAEPARRVP
                     ETLTRAARYRAAGASGLFVPGITDAREIAAVVGDCGLPVNVLAWTGLPDADRLRALGV
                     RRLSAGSAISEATQGLVLGMMKQFLATGALADGDTGPLDYGTVQALMARR"
     gene            complement(7581..9131)
                     /locus_tag="VIN75_05650"
     CDS             complement(7581..9131)
                     /locus_tag="VIN75_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8973681.1"
                     /translation="MALRLDSLKLARRLALGFCALLTLVICIATVGVRALSDAQADSA
                     RTNAAMRQAADADRWRGMTQLNISRTLALAKSGNAAALKTWSAPLMKETSAEISIVQK
                     GLEAAAASPEAKAAFADIAAKRKRYVDTRDQVFKLLDASDATANDLVETRLLPQAGDY
                     MAAVTAFGKQQREVSEAVAAQSNALADRARITVIALAVVSMLLGALCAWFITRSVTAP
                     LEQAVSAVEAVAAGDLSRAIAVEGRDEVAALLNGLNRMQASLRQLVGDVRETTSSIQV
                     ASSEVAMGSQDLSARTERSAAGLQQTASSMEQISGSVRQTAAAAQVADDLAAATARQA
                     AAGGDVVSRMTSTMDRITAHSNRIGDIIGVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVASEVRSLAQRSSAAAAQIKVIIGESGQAVAAGAALVHDACSSMSEINSSVAKVS
                     AAIGEIRNASSEQADGVGQVNLAVANLDQATQQNAALVEETAAAADSLKEQAIRLSDA
                     MAQFKLAA"
     gene            9323..9844
                     /locus_tag="VIN75_05655"
     CDS             9323..9844
                     /locus_tag="VIN75_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8973682.1"
                     /translation="MHRPLNFHHLMRDHSLAVDQGRLTETEAATAVARAIQQSLACAR
                     VNFWNVSGPLGRRVMRRVTGYDGRAERVLSEPLELQETTGEFFNKLREAGCHVCADTA
                     TDPLLRDIFRTYLQPTQSVTMIVASFHVDEQVWGLISCVHDSPRRWTAAEVTALRKCA
                     SELSRRRLQLAER"
     gene            complement(9879..10520)
                     /locus_tag="VIN75_05660"
     CDS             complement(9879..10520)
                     /locus_tag="VIN75_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY8973683.1"
                     /translation="MARRVVVFDLDGTLSNPLDGFARSINHALRLQGLPEHEPQALAR
                     YIGPPLYESAVELTGSDDPTVHNRLIADYRHRYGESGFAENTLYDGIPEVLHALRDAG
                     ATLGLCTSKRVDFAEAILRNFGLRELFAFVSGGDIGVHKWQQLQALREAGAVDADSVM
                     VGDRDVDITSAHRNGLLACGVLWGFGSREELQAESPRHLAETPADLRRLAQAW"
     gene            complement(10526..11053)
                     /locus_tag="VIN75_05665"
     CDS             complement(10526..11053)
                     /locus_tag="VIN75_05665"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY8973684.1"
                     /translation="MKVALIAAVARNGVIGHDNALLWKLPEDMAFFKRTTMGNPVIMG
                     RRTWDSIPPRFRPLPGRANIVLTRQKDWQADGAAVAHDLEQALEMALESVSSNPHGLR
                     AFVIGGAELYALALPHADELVLTEIDKDFEGDTRFPAWPRNHFVETGRERHHAAAPND
                     FDFAFVSYRRTHAAR"
     gene            complement(11050..11880)
                     /locus_tag="VIN75_05670"
     CDS             complement(11050..11880)
                     /locus_tag="VIN75_05670"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY8973685.1"
                     /translation="MTRPVRSQYEDFMRLVRDQGRFKADRTGTGTTSLFGHQMRFDLA
                     EGFPLVTTKKVHLKSIILELLWFLRGDSNVKWLQDRGCTIWDEWAREDGDLGPVYGVQ
                     WRSWPTPDGGHVDQIAEVVKQLRTNPDSRRIIVSAWNVADLPRMALMPCHAFFQFYVA
                     PPETEGGRGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLDVGDFIWTGGDC
                     HLYANHREQVDTQLARTPLPYPTLHIKRRPASILDYEYEDFEVLDYQHHPAIKAPVAV
                     "
     gene            12039..>13020
                     /locus_tag="VIN75_05675"
     CDS             12039..>13020
                     /locus_tag="VIN75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY8973686.1"
                     /translation="MLDEPDGARGSSAAGAAWLPAAQVGQSLGIVPHDGTRSAEEVSH
                     GTNVQVTTGQAGVLARVRAGARTALAGVLAAVALGAAANPLPTAAPAAPAAASAAAAS
                     IGWGRMANTVFEHLGQHDGLPHAIATGVTEDAQGYLWASTQDGVARWDGYRFKVYPAR
                     PDDPHGLPVSYVRTIAGDRQGRIWVGMDGGGVARFDAALNGFIRFGGSTVVQLAPDAE
                     GGMWAASTTGLMHVRASGKVEDAWPHGGKVDPLPEASITAVLQDHGGAVWVGTASGLV
                     ERFPDGRPGRRIDLSGGADGGAVPVDSLYEDAHGQIWIGTNLHGVMVLDPA"
BASE COUNT         1834 a         4568 c         4717 g         1901 t
ORIGIN      
        1 gcgctgaggc gctcgcgcac gacgcccgcc gtacagcgcg gtcgccgacg atctccaccc
       61 aggcgtcctg gccccgacgc cgtcgcggtc ttgcgaagta cgacgggcgt cgtataaagt
      121 acgatgactg tcgtacttcg aggagatccg atggcttggc ggctgcgctt ggtgaactcg
      181 tccgtgttga acgtcgccac cgcagccgcg gtcgtgctgg cggcctcggg cccgcaagcg
      241 gcccatgccg cggtcgatcc cgtgaaggcg caggccctgt tcgacgaggc cgcgcggttg
      301 tgccaggccg acggcggggc gttgtggcat cactcgctgt gcgggcccat cctgctggtc
      361 gactggaccg acaacaccgc ggtcgccaac cagcccgatc cggccggcgt gctcgagccg
      421 gtgggcaagg tgttcgcggg cacgctgccg gcggacgtcg tcatcgccaa cacgccgatc
      481 gaatggtcgg gcaagcgctg gaccgagctg ctctggccgc tgcccgacga cgtggcgcat
      541 cgccacgtga cgctctcgca cgagctgttc catcgtgcgc agcacgagct gcagatgccc
      601 atgggcgacg gcggcaacct gcacctggac acgctcgaag gccgcacctt gcttcagctc
      661 gaatggcacg cactggccag ggccctggtc gcgccggacg ccgtggcgcg caacgccgcg
      721 gtgtcggatg cgctgctgtt ccgccatgag cgatatcgcc tgttccccga cgcgcgggcg
      781 gaggagcacg cgctcgagct caacgagggc gtggccgagt acaccggcgt gcgcgtgggc
      841 ctgccgaccg cccgtgaacg cacggcgtat gccttgcgcg acctcgaatg cttcatccag
      901 tcgcccacct tcgtgcgctc gttcgcgtac gccaccggac cggcctgggg cctgctgctc
      961 gacaaggcag accctgcgtg gcgcgacgag ctcgccgccg cgatgaaggc gggccacccg
     1021 cagggactcg acgacatgct gcaggccgcc ttgaagctgc cggagccgtc gccggccacc
     1081 gtgaaggcgc gcgaagccga ctacgacgac acgctgcgcc cgcgcgaaca ggcgcgcgac
     1141 caggcccggc aggcgcacct ggccgagctc aaggccaggc tggtcgacgg ccccgtgctg
     1201 cggctgccgc tggcgcacct gcacgccagc taccagttca atccgcagac cctcgaggcg
     1261 ctgggcagcg acggcgtggt ctatccgacg atgacgctga gcgccgactg gggcacgttg
     1321 aaggtggagc agggggcctt gctggacaag gcgatgagcg gggcggcggt ctccgcggcg
     1381 ggctcgtcgg ccgatcacct ccagggcccc ggctggcgcc tgacgctggc caagggctgg
     1441 accgtggcgg ccggggagcg gaacggtgat ttcgtcgtga agcaggagag cgactcgcac
     1501 tgaccccgcg cgagccggga cggcaaccga ccgcgcgctg cggtccggcc gcggccgcgc
     1561 tgtcccgttg gtggcaacgc agcccggccg tcgcgccgat aatccggcgc gatgagcgaa
     1621 gccccgaaca ccctggcatg ggacgcgccg cgcccgcacg tgctgccggt ggtcgtcaac
     1681 gacggccaca tcgacctgat gcagcacgtc aacaacgtgc actacctgca gtggctggag
     1741 aacgtcgcgt gggaccactc ggtcgcgctg ggcatgcggc cggaggacta cacgcgcctg
     1801 ggccagggca tggtggtacg ccggcacgag ctcgactacg tcgcgcccgc gctgctgggc
     1861 gaccgcgtgc tgctggcgac gtggatcgtc gggctcgacc ggctgtcgct gcaccggcgc
     1921 taccagttcg tgcgcgagtc cgacggcgtg acgctgttcc gcggcgcgac gcacttcgtc
     1981 tgcgtcgaga tcgcctcggg cagggtgcgg cgcatgccgc cggacttcac cgaggtctat
     2041 gcccgcggct tcatgccgtg gccgtcgccg ggcgcttgac gatgcccttg ccgtcctcgt
     2101 gcgcgagcgg caccgacgtg tgctcgtgca ccacctgcca gcggccgccg cgctggcgca
     2161 gcacccaggt caggcggttg tcgagcgagc gcagttcctg gcctgccggg tcgacggcgc
     2221 ggtaggtgag gctggcggtc agcatcgcca tgtcgcccga cacctgcgtg cgcacgccgt
     2281 gcgcctcgac cacggagcgc tggtcgccca ggaaggcgaa ccagcccttg gccatctcgc
     2341 gccaggatcc gatgtcgtgc acccacgtgc cccacagctc gaagatctgc gcgtcctcgg
     2401 aatagaccga gacgaaggcg tccacgtcct tggccagcac ggcggcctgg taggcggcca
     2461 gcgcggcccg gatgggttga tcagcgtcgt tcactcgagg tctccccgac gtcgtccgaa
     2521 gggcggagta tccgacgatt tcaggacgtc gcggcaggga agtgctcgac gtgcgccttg
     2581 acgcgccggc cgaagtacgt gacggatcca cctccacctt gcggcgaggc gtcgtggcgg
     2641 aggtggatcc tgcgactgcg cacaggatga cgcttggtgg gcgcgaagcg gcaacagtgc
     2701 atcacgcagg gtgcgccaac ctgcggcaat ggtcacgcgg agcgcaccaa cctgcggtag
     2761 tgatcacgcg gagcgcgcca acctgcggca gtgatcacgc cgagcgccaa tctgcgtcag
     2821 cggtcgcgcg gcgcgccaac gccgtcatcc tgcgcgcagc gaagcgaagt cgcaggatcc
     2881 acccctgcgg cgaagcatcg tggcgaggtg gatcctgcga ctgcgcagga tgacagcttg
     2941 gggtgagcga acgtcccggg cgggagcgag cgacgagcgg cgatcgcgcc gctcgtccac
     3001 ctcagatccc cgcgtaccac tcgtacccgc gatcctccca gaacccgccc ttgccgccgt
     3061 tcaggtgcgc gtacgcgtcg gccacctcca cgcgcatcac gtacttggcc tgcttgtagc
     3121 cgagctggcg ctcgacgcgc aggcgcagtg gcgcgccgtg cgccaccggc aggtcctggc
     3181 cgttcatgcc gtaggcgagg atcgtctgcg ggtggtaggc gtccagcagg tcgatgctct
     3241 cgtagtacag gcccgagtcg tcggtcgtcc cggccaggtt gtccgcgcag tggaagatga
     3301 cgtagcgcgc gcctggcttc aggcctgcga ggtcgagcac ctgcgacagc ggcacgccct
     3361 gccacttgcc gatcgcgctc cagccctcga cgcagtcgtg gcgcgtgatc tggctgcgcg
     3421 ccttcatggc cttgagctcg gccagcgaca gcgacagcgg ccgctgcacc aggccgtcga
     3481 cctgcaggcg ccagtcggcg aacctgccct gcagcaggcg cgcgtactcc gggctggacg
     3541 gcatcgacga gccgttggcg cggaacaccg gcgacaggtc gcgctcgccg tattcgcggg
     3601 cgagcggctg gtggtccagc agcaggcgct ggctggacaa ggtcagcttc tccgtacctg
     3661 ccagcgtgcc ggcgatgcgc gggtcctcga cgaggcggtc gcagccgccg agcgcgagcg
     3721 tgccggcgcc cgcggccagc gtcgtgagcc agcggcggcg cgactggaac ggcgtcatgc
     3781 ggcccttgcg gaaggggacg ttcgggttca tcgcgcgtcc tctttggtct cgatcgcgta
     3841 gcgtccggtg atcatcgagc gcaggttgtt ccacacgccc gacaccagca ccatcacaag
     3901 gtgcaccacg acgaaggcga ccagcgccga cgcgaagatg aagtgcagcg aacgcgccga
     3961 cggccggccg ccgaacaggt ccagcaggaa cgggaaggcc gagtccaggc ccggcgacat
     4021 cgtcaggccc gtcagcacca tgcccggcag cagcacgaat gcgacggcca ggtagctcag
     4081 cttctgcagc acgttgtagt ggcgcgcggc ctcgcccttg gggaaatcca gcttcgcgtg
     4141 gtcgacgact tcgcgtgcga ggtgcgacgg ggcgagctcg gcgcgcgtgg gcagcaggtc
     4201 gcgccggaag tgccgcgaca gcaggccgcc cacgaggtag acggcgccgt tgatcgcgaa
     4261 cagccaggcg aagaagaagt gccagtgccg gccgtcggcc aggtcctggt acgacgggat
     4321 cgtcagccac gacgggaagc cgcgcggctg caggttgccg gcggcgtccc tggacgcgcc
     4381 cagcacgccg gtggtctcga ccgtcagcga cccgacgtgc agcacgccgc gccagtggcc
     4441 gttgctgcgc agcgagtcca tcgacagcca cgcgtggtcg tggtcggcgc cgtattcgcc
     4501 ccagtacagg cgcgggtggg cgttgaagat ctgcgcgccc gacatcagca gcaacgagaa
     4561 gcacaggacg ttgatccagt gcgtcaggcg caccagcacc gaatggcgcg gcacgagctg
     4621 gcgcgccggc cgggcggcgc gttggcggaa catgccggag ggcatcctgg cggtggtcat
     4681 ggcgggaggc gaggtcatgg ccgttggtcg gacggcgaag ctggacctta caccacatgc
     4741 cgccatttgt agggcgggtt ggccgcaggc gcaacccgcc aggcggccga tcacgacgcc
     4801 aacgaaccgc aggccaagcc tgcggcaaat ccgccctacg cggccgggct ttcggggtac
     4861 aacccatgcg cctggccaaa gacccgcgcc agcccgaaga tcgcatcgat cagcggcgtc
     4921 tccacgccca cctgcgcccc cagttcgcgc accgcgccga ccaggctgtc gagctcgatc
     4981 gcgcggccgg cctcgacgtc ctgcagcatc gacgtcttga acgcacccag cttgcgcgtg
     5041 atggcgtggc gttcctcggg cgactgcgtg atggcaaagc ccaggcgctc gccgatgcgg
     5101 cccgactcca gcatcgcgcc gctggtgagc ttgcgcacga gctcgtcgcc gatgatccgg
     5161 tccgtcgtgg cgccggtgat ggcgctgatc gggttggtgg tcatgttgcc ccacagcttg
     5221 taccagatgt cgcgctggat catctcgctg gcggtgcact cgaagccggc cgcatccagc
     5281 gcggccttca gggcggcgac gcgcccggaa accccgccgc gcggctcgcc gatgaccagc
     5341 ccgcggccca tctggttgtg cgccttgccg ggctcgacga ccgacgccgc ggcgtgcacg
     5401 acgcaaccga tcacctgttc cagcggcagg tggcgcgcga tgctgccgtc ggggtcgacg
     5461 ctcttgagcg cgagcccggc cgcggggccg gacagcccgt ggcagaacca ccagggcacg
     5521 ccgttcatcg cgggcacgat cagcgtgcgc ggctccagca gcggcgcgat gtggcggccg
     5581 acatcggcca gcgccggcgc cttcaccgcg atcacgacga cgtcctgcac gccgagcgcg
     5641 ccggggtcgg cgctggcggc gaccggcccc gagatcggac cggcgaagcc gtccacgcgc
     5701 aggccgtgct cgttcaatgc ggccagcgtc gcgccgcgcg cgaccgcgct cacctggtgc
     5761 tggccggtgg cggccaggcg cgcgcccacc agcaggccga tcgcgccggc gccgtagatg
     5821 cagactttca tcggaagacc tctcggaagc tgtgaatgaa tccggcgcgt ccgagcggcg
     5881 cgccgagact cgtctgatct aggcgcaaag cataggcgtg gcccgggcca cggcgagcat
     5941 ttgcaacgac gagcaggcgt gttttggcct gccgagcggg cgtgcgggat tcgttcacag
     6001 cttctcagac cttgaggaac gcggcgatgc cgatcgcgat gcccacgccg gccacgccca
     6061 gcgtgcacca ttcgagccag cggcggcgcg tcagcagcgc gatggcggcc agcgcgatcg
     6121 ccacctggat cgcggtcgtc gcctcggccc agcggtggtg ctggtgcatc aggtgctcgc
     6181 tcctgtcgtc gaagtccttg gcctcggcct cgagcgcctc ggccttggcc ttgatgggct
     6241 ccttgtcgga cttgtacttc tcgatgttct tcgcgtacgc gtcgcggcgc tcggccggca
     6301 cgatgtccag cgcgacctcg gcgatcgcct ggcgcgcgtt cttggcctgg tagaagttcc
     6361 actggtccga cgcctcggtc ttgcggatcg cggcgtcgtt cttggcgagc gcggcgtcgg
     6421 cctgcgtggc gccgcccatg tacgagaaga gggcgccgac ggtggcgagc acggcggtga
     6481 tgacggccag ctggccggtc atgccccggg cgccgtgctc gatggcgtgc tcgacctcgt
     6541 ggtcatgggg gccgtgcacg tggaatcctg agctcatgga gggtctttcg agtggggtgg
     6601 cgacggcgat ctgcgtcgcc cagccggcga tggtagcgaa gaacgctgaa cgccccgcga
     6661 ccgtggcgac gcgggaacac acgaccgccg gatggacatg tccgcaagcg gcgagtccgc
     6721 cgggggcgcc cgtgccaaca tcgccgatcg acaacgacag gccccccatg aagcccgaac
     6781 tcgccacccg attccaccac ctgcatgccg atggcctgct gctgctcgcc aacgcctggg
     6841 acgccggcag cgcgcgcctc gcgcaggccg ccggcgcgcg cgccgtcgcc accagcagcg
     6901 ccgccgtcgc ctgggcccac ggctggcctg acggcgacgc gctgcccctc gaactcgtgc
     6961 tgcagacgac gcgcgcggtc gccgcggccg tcgacgtccc ggtgacggtc gacatcgaag
     7021 gcggctactc ggacgatcca gcgcgcgtcg gcgagctcgt cgcccaggtg gtcgccgcgg
     7081 gcgccgtggg catcaacatc gaggacggca gcggcgatcc ggcgctgctg gccgccaaga
     7141 tcgcggccgc gcgccaggcc gcgacggcgc tgggcgtcga cctgttcatc aacgcgcgca
     7201 ccgacgtctg gctgcgcggc ctcgcggagc ccgctcgccg cgtgccggag acgctcacgc
     7261 gcgccgcgcg ctaccgtgcc gcgggcgcga gcgggctgtt cgtgccgggc atcaccgacg
     7321 cccgcgagat cgccgccgtc gtgggcgact gcggcctgcc cgtgaacgtc ctggcctgga
     7381 cgggcctgcc cgatgccgac cggctgcgcg cgctgggcgt gcggcgcctg agcgcgggct
     7441 cggccatctc cgaggcgacg cagggcctcg tgctgggcat gatgaagcag ttcctggcca
     7501 ccggcgcgct cgccgatggc gacaccggcc cgctcgacta cggcaccgtg caggcgctga
     7561 tggcgcgccg ctgacggccc tcaggccgcg agcttgaact gcgccatcgc gtccgacagg
     7621 cggatggcct gttccttgag gctgtcggcc gcggcggccg tctcctcgac cagcgcggca
     7681 ttctgctgcg tggcctggtc caggttggcg accgccaggt tgacctggcc gacgccgtcg
     7741 gcctgctcgg acgacgcgtt gcggatctcg ccgatcgccg ccgacacctt ggcgacggag
     7801 ctgttgatct cggacatcga gctgcaggcg tcgtgcacca gcgccgcgcc ggccgccacg
     7861 gcctggcccg actcgccgat gatcaccttg atctgcgcgg cggcggccga cgatcgctgc
     7921 gcgaggctgc gcacctcgct ggccacgacc gcgaagccgc ggccctgctc gccggcgcgc
     7981 gccgcctcga cggccgcgtt cagcgcgagg atgttggtct ggaacgcgat gccgtcgatg
     8041 acgccgatga tgtcgccgat cctgttggag tgcgcggtga tgcggtccat cgtcgacgtc
     8101 atgcgcgaga cgacgtcgcc gccggcggcg gcctggcggg ccgtggccgc cgccaggtcg
     8161 tcggccacct gggcggccgc ggccgtctgg cgcacgctgc cggagatctg ctccatcgac
     8221 gaggcggtct gctgcaggcc cgcggccgag cgttcggtgc gcgccgacag gtcctggctg
     8281 cccatggcca cctcggaact ggcgacctgg atggagctgg tcgtctcgcg cacgtcgccc
     8341 accagctggc gcagcgacgc ctgcatgcgg ttcaggccgt tgagcagggc ggcgacctcg
     8401 tctcggcctt cgacggcgat cgcgcggctc aggtcgccgg cggcgacggc ctcgaccgcc
     8461 gacacggcct gctcgagcgg cgccgtgacc gagcgcgtga tgaaccaggc gcacagcgcg
     8521 cccagcagca tcgagacgac ggccagcgcg atcaccgtga tgcgggcgcg gtcggccagc
     8581 gcgttgctct gggcggccac ggcctccgag acctcgcgct gctgcttgcc gaacgccgtc
     8641 accgcggcca tgtagtcgcc ggcctgcggc agcagcctgg tctcgacgag gtcgttggcc
     8701 gtcgcgtccg acgcgtccag cagcttgaac acctggtcgc gcgtgtcgac gtagcgcttg
     8761 cgcttggccg cgatgtcggc gaacgccgcc ttggcctcgg gcgacgcggc ggccgcctcc
     8821 aggcccttct gcacgatgga gatctcggcc gaggtctcct tcatcaacgg ggcggaccac
     8881 gtcttgagcg ccgcggcgtt gccggacttg gccagcgcca gcgtgcggct gatgttcagc
     8941 tgcgtcatgc cgcgccagcg gtcggcgtcg gcggcctggc gcatcgcggc gttcgtgcgg
     9001 gcgctgtcgg cctgcgcgtc ggacagcgct cgcacgccga ccgtcgcgat gcagatcacg
     9061 agggtgagca gggcgcagaa gccgagggcg agccggcggg cgagtttcag ggaatcgaga
     9121 cgcagggcca tatcaaactc cgatgacgta ccggatttcg accggcctgc ccgaaacttg
     9181 ggcgcggtgg ccattgcatt tcctttgttt ccggctgctt cagtttgaag acagctattt
     9241 gcgcaacaga cccggggttc accgccttgg ccggcgctgc ggtcgagcca agaatgtggg
     9301 cgattcgccc ctcagagttc gcgtgcatcg tccgctcaac ttccaccact tgatgcggga
     9361 ccactcgctg gccgtcgacc agggccggct gacggagacc gaggccgcga cggccgtggc
     9421 gcgcgccatc cagcagtcgc tcgcctgcgc gcgcgtgaac ttctggaacg tctcgggccc
     9481 gctgggccgg cgcgtgatgc gccgcgtcac gggctacgat ggccgggccg agcgcgtgct
     9541 gtccgaaccg ctggagctgc aggagacgac gggcgagttc ttcaacaagc tgcgcgaggc
     9601 cggttgccac gtctgcgcgg acaccgccac cgacccgctg ctgcgcgaca tcttccgcac
     9661 ctacctgcag ccgacgcaga gcgtgacgat gatcgtggcg tcgttccacg tcgacgagca
     9721 ggtgtggggc ctgatctcat gtgtccacga cagtccgcgc cgctggacgg ccgcggaggt
     9781 caccgcgctg cgcaagtgcg cgtcggagct gtcgcggcgg cggctgcaac tggccgaacg
     9841 ctgagcggcc cgcgcggtca gcgctggcgg tcggccgatc accaggcctg ggccagccgc
     9901 cgcaggtccg ccggcgtctc ggccagatgg cgcggcgatt cggcctgcag ctcctcgcgc
     9961 gagccgaaac cccacagcac gccgcacgcg agcaggccgt tgcgatgcgc ggacgtgatg
    10021 tcgacgtcgc ggtcgcccac catcaccgag tcggcgtcga ccgcgcccgc ctcgcgcagg
    10081 gcctgcagct gctgccactt gtgcacgccg atgtcgccgc cgctgacgaa ggcgaagagt
    10141 tcgcgcaggc cgaagttgcg caggatggcc tcggcgaagt ccacgcgctt ggacgtgcac
    10201 aggcccagcg tggcgccggc gtcgcgcagc gcgtgcagca cctcggggat gccgtcgtac
    10261 agcgtgttct ccgcgaaacc ggattcgccg tagcggtggc ggtagtcggc gatcaggcgg
    10321 ttgtgcacgg tcgggtcgtc gctgccggtg agctcgacgg ccgattcgta caggggcggc
    10381 ccgatgtagc gcgcgagcgc ctgcggctcg tgctcgggca gtccctgcag gcgcagcgcg
    10441 tggttgatcg agcgcgcgaa gccgtccagc gggttgctga gcgtgccatc gagatcgaag
    10501 acgacgacgc gacgggccat cggggtcagc gtgcggcgtg cgtgcgccgg tagctcacga
    10561 acgcgaagtc gaagtcgttc ggcgcggcgg cgtggtggcg ctcgcggccg gtctcgacga
    10621 agtggttgcg cggccacgcg gggaagcgcg tgtcgccctc gaagtccttg tcgatctcgg
    10681 tcagcaccag ctcgtcggcg tgcggcagcg ccagcgcgta gagctccgcg ccgccgatca
    10741 cgaacgcgcg cagtccgtgc gggttggacg acaccgactc cagcgccatc tccagcgcct
    10801 gctcgaggtc gtgcgcgacg gcggcgccgt cggcctgcca gtccttctgc cgcgtgagca
    10861 cgatgttggc gcgcccgggc agcggccgga agcgcggcgg gatcgagtcc caggtcctgc
    10921 ggcccatgat caccgggttg cccatggtgg tgcgcttgaa gaacgccatg tcctccggca
    10981 gcttccacag cagcgcgttg tcgtggccga tgacgccgtt gcgcgcgacc gcggcgatga
    11041 gcgcgacctt cataccgcca ccggcgcctt gatggccggg tggtgctggt agtcgagcac
    11101 ctcgaagtcc tcgtactcgt agtcgaggat cgatgccggg cggcgcttga tgtgcagcgt
    11161 cgggtagggc aggggcgtgc gcgcgagctg ggtgtcgacc tgctcgcggt ggttggcgta
    11221 caggtggcag tcgccgccgg tccagatgaa gtcgccgacg tccaggtcgc actgctgcgc
    11281 cagcatgtgc gtgagcaggg cgtagctggc gatgttgaac ggcacgccca ggaagatgtc
    11341 cgcgctgcgc tggtagagct ggcagctgag cttgccgcgg ccgccttcgg tctcgggcgg
    11401 cgccacgtag aactggaaga aggcgtggca gggcatcagc gccatcctgg gcaggtcggc
    11461 cacgttccag gcgctgacga tgatgcggcg cgaatccggg ttggtccgca gctgcttgac
    11521 gacctcggcg atctggtcga cgtggccgcc gtcgggcgtc ggccaggagc gccattgcac
    11581 gccatagacc gggcccaggt cgccgtcctc gcgcgcccat tcgtcccaga tcgtgcaacc
    11641 gcgatcctgc agccacttga cgttggagtc gccgcgcagg aaccacagca gctcgaggat
    11701 gatggacttc aggtgcacct tcttggtggt caccagcggg aagccctcgg cgaggtcgaa
    11761 gcgcatctgg tggccgaaca ggctggtggt gccggtgccc gtgcggtcgg ccttgaagcg
    11821 gccctggtcg cgcaccaggc gcatgaagtc ttcgtattgc gagcggacgg ggcgggtcat
    11881 gggccgattt taccggtcga gccctgcccg cccggggcgc cagggattgg cgcttgcagt
    11941 gccagcgcca cgcgggatcg ttcatacgtc acgccgggct gtcgggattt cccgcgatcg
    12001 tgcgcaaagt ccttcatcgt cgcgcgggcg cgccgacaat gctggatgag cctgacggtg
    12061 cccgcggctc atcggcggcg ggcgctgcgt ggctgccggc cgcgcaggtc gggcaatcgt
    12121 tgggcatcgt gccgcacgac ggcaccagga gcgcggaaga agtgagccac gggacgaacg
    12181 tccaggtgac gaccggtcag gcgggtgtgc tggcgcgcgt gcgcgccgga gcccgcaccg
    12241 cgctggccgg cgtcctggcg gcagtggcgc ttggcgcggc cgccaacccg ctcccgaccg
    12301 ccgcgccggc cgctccggcc gccgcctccg ccgcggcggc cagcatcggc tggggccgca
    12361 tggccaacac cgtcttcgag cacctgggcc agcacgatgg cctgccgcac gccatcgcca
    12421 ccggcgtcac cgaggatgcc cagggctacc tgtgggccag cacccaggac ggcgtcgccc
    12481 gctgggacgg ctaccgcttc aaggtctacc ccgcccggcc ggacgacccg cacggcctgc
    12541 cggtcagcta cgtccgcacc atcgccggcg acaggcaggg ccgcatctgg gttggcatgg
    12601 acggcggcgg cgtcgcccgt ttcgacgccg ccctcaacgg attcatccgc ttcggcggct
    12661 cgacggtggt gcagctggcg cccgacgccg agggcggcat gtgggccgcc agcaccacgg
    12721 gcctgatgca cgtgcgggcg tccggcaagg tcgaggacgc ctggccgcac ggcggcaagg
    12781 tcgatccgct gcccgaggcg tcgatcacgg ccgtgctgca ggaccacggc ggcgccgtct
    12841 gggtcggcac cgcctccggc ctggtcgagc gcttccccga cggccgcccc ggccggcgca
    12901 tcgacctgtc cggcggtgcc gacggcggcg ccgtgccggt cgactcgctc tacgaggacg
    12961 cccacggcca gatctggatc ggcaccaacc tgcacggcgt gatggtgctg gacccggcga
//
LOCUS       DATCRO010000209         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_350757, whole genome shotgun sequence.
ACCESSION   DATCRO010000209 DATCRO010000000
VERSION     DATCRO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350757"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..644
                     /locus_tag="VIN75_05680"
     CDS             159..644
                     /locus_tag="VIN75_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973687.1"
                     /translation="MDSNSHLVAHGQGRRARHALVWGCVLLLATGVAWLLGHDVWLQQ
                     TPFGPQPHPLVAWLLRAHGAIAWCVTLVGGTVWQVHVRPAWRAVRRRRLHRRRVEGSR
                     GRTVSGVLLVVALAVLLGSAIGLQYAPEDAHAALSVTHWGLGLALAAGLLWHWIARLR
                     R"
     gene            689..2398
                     /locus_tag="VIN75_05685"
     CDS             689..2398
                     /locus_tag="VIN75_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8973688.1"
                     /translation="MTAALPTPDFTRFPRHAELTTLLQAYAAARPELVDLRSIGRSHE
                     GRDIWAVVLTRKATGADIDKPAFWIDGNIHAAELTACTAALYYLHHVLANEAHDARLA
                     HLLDTRAVYLVPRLNPDGAELALADTPRFIRSSTRSYPFDEDAPDGMTMEDVDGDGRV
                     LYMRLRDPHGTYKKHPAEPRLMVAREPGEIGGEYFRVIPEGTIKAWDGVRVTVNRDRE
                     GLDLNRNFPSLWRQEFEQVGAGPYPTSEPEVRAMVDFCVAHPNIGAAVSFHTHSGVIL
                     RPMGTTADDDMTPEDLWMIKRLSDIGANLTGYPAISGFHDFKYHPKEVITGTQDWLYE
                     HQGALCWTVEIWAPNKEAGIDKYDWIHWYRDHPPEDDLKLLKWSDEQCGGQAYVDWKP
                     FRHPQLGDVEIGGWDRMNFWRNPPPALREREAARFPAWLTQLALSLPRLEILQATCEA
                     VGHGGDGDTWRVRLAVGNAGYLPAYVTHRAKERKVVRGTVFEIALPAGASLVTGKERE
                     IGEHLEGHAPRSSLQAFLPDRDVTADRTLQEWIVRGPRGAVVTVDARADRAGAVSARI
                     ALG"
     gene            2501..2875
                     /locus_tag="VIN75_05690"
     CDS             2501..2875
                     /locus_tag="VIN75_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973689.1"
                     /translation="MLTFILIVLAILAFTVVPVMIAARIVGAGRTGFWICFGAMIVSL
                     LIVGFAARIFHIGGLLGLFVAPLGYMWILDTTYLRGLAMVFLQYLIVAAFAVILAFTA
                     LGSMLHVKDMMRHLDTAPAQSV"
     gene            complement(2949..3368)
                     /gene="yiaA"
                     /locus_tag="VIN75_05695"
     CDS             complement(2949..3368)
                     /gene="yiaA"
                     /locus_tag="VIN75_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HEY8973690.1"
                     /translation="MSANANKPTAAFVGASWTALIAGAGAYLFGLWNTNMQLNEKGYY
                     FTLLMYGLFAAVSLQKTVRDRLEGLPVTGLYAGLCWLSMAITITLMGVGLYNAQLALS
                     EKGFYGMAFLLALFATIAIQKNVRDSQVPVRRASRDE"
BASE COUNT          501 a         1295 c         1256 g          516 t
ORIGIN      
        1 gtcccgcgtt cggtgaccgt gctcgcgccc acggcctgcg cagccgacgc gctcaccaag
       61 atcgtctggc agcagggcga gctcgcgggc gcgctactgc gcaaggcccg tgcgcgcgcg
      121 ctcgtcgtgc gcgcggacgg cagcgtctgg cagagctgat ggactcgaac agccacctcg
      181 tcgcgcacgg ccagggccgg cgcgcgcgcc acgcgctggt ctggggctgc gtgctgctgc
      241 tggccacggg cgtggcatgg ctgctggggc acgacgtctg gctgcagcag acgccgttcg
      301 gcccgcagcc gcatccgctg gtggcctggc tgctgcgcgc gcacggcgcg atcgcgtggt
      361 gcgtgaccct ggtgggcggc acggtctggc aggtgcacgt gcgacccgcc tggcgcgccg
      421 tgcggcgccg gcgcctgcat cggcgccgcg tcgagggcag ccgcggccgc acggtcagcg
      481 gcgtgctgct ggtcgtcgcg ctggccgtgc tgctggggtc ggccatcggg ctgcagtacg
      541 cgcccgagga cgcgcatgcg gcgctgtcgg tcacgcactg ggggctcggg ctggcgctgg
      601 cggccggcct gctctggcac tggattgccc ggctacgtcg ctgagcgcgc gcggcgcaga
      661 atggccgatc gccattgccc gcgccaccat gaccgccgcc ctgccgacgc ccgacttcac
      721 ccgctttccc cgccacgccg aactcaccac gctgctgcag gcctacgccg ccgcgcgtcc
      781 cgaactcgtc gacctgcgct cgatcggccg gagccacgag ggccgcgaca tctgggccgt
      841 cgtgctcacg cgcaaggcca ccggcgccga catcgacaag cccgcgttct ggatcgacgg
      901 caacatccac gccgccgaac tgaccgcgtg cacggcggcg ctgtactacc tgcaccatgt
      961 gctcgccaac gaggcgcacg acgcgcggct cgcgcacctg ctcgacaccc gcgccgtcta
     1021 cctcgtcccg cgcctgaacc ccgacggcgc ggagctggcg ctggcggaca cgccgcgctt
     1081 catccgctcg tccacgcgca gctacccgtt cgacgaggac gcccccgacg gcatgacgat
     1141 ggaggacgtc gacggcgacg gccgggtgct ctacatgcgc ctgcgcgacc cgcacggcac
     1201 gtacaagaag catccggccg agccgcgcct gatggtggcg cgggaaccgg gcgagatcgg
     1261 tggcgagtat ttccgcgtca tccccgaggg cacgatcaag gcctgggatg gcgtgcgcgt
     1321 gacggtcaac cgcgaccgcg agggcctcga cctgaaccgc aacttcccgt cgctgtggcg
     1381 ccaggagttc gagcaggtgg gcgccggccc gtaccccacc agcgagcccg aggtgcgcgc
     1441 gatggtggac ttctgcgtgg cccatcccaa catcggcgcg gccgtcagct tccacacgca
     1501 cagcggcgtg atcctgcgcc cgatgggcac caccgccgac gacgacatga cgccggaaga
     1561 cctgtggatg atcaagcgcc tgtcggacat cggcgcgaac ctcacggggt atccggccat
     1621 cagcggcttc cacgacttca agtaccaccc caaggaggtg atcaccggca cgcaggactg
     1681 gctctacgag caccagggcg ccctgtgctg gacggtcgag atctgggcgc ccaacaagga
     1741 agccggcatc gacaagtacg actggatcca ctggtaccgc gaccacccgc ccgaggacga
     1801 cctgaagctg ctcaagtgga gcgacgagca gtgcggcggc caggcctacg tcgactggaa
     1861 gcccttcagg cacccgcagc tgggcgacgt ggagatcggc ggctgggatc gcatgaactt
     1921 ctggcgcaac ccgccgccgg ccctgcgcga acgcgaggcg gcgcgcttcc cggcgtggct
     1981 cacgcagctg gcgctgtcgc tgccccggct cgagatcctg caggccacgt gcgaggcggt
     2041 cggccacggc ggcgacggcg acacatggcg cgtgcggctg gcggtcggca atgccggcta
     2101 cctgcccgcg tacgtgacgc accgcgccaa ggagcgcaag gtggtgcgcg gcacggtgtt
     2161 cgagatcgcg ctgccggccg gcgcgtcgct ggtcaccggc aaggagcgcg agatcggcga
     2221 gcacctcgag ggccacgcgc cgcgcagctc gctgcaggcc ttcctgcccg atcgcgacgt
     2281 caccgccgac cgcacgctgc aggagtggat cgtgcgcggg ccgaggggcg cggtcgtgac
     2341 ggtcgatgcg cgcgccgatc gcgccggggc ggtcagcgcg cgcatcgcgc tggggtgacg
     2401 cccggcgtcc ttacgcgatc ctgatgccgt cgacgcggcc gccgggccag aatggcgccg
     2461 ccaaggcgcc tcacgacatc tgcaggaagg atctcccgcc atgctgacgt tcatcctcat
     2521 cgtcctcgcc atcctggcgt tcaccgtcgt cccggtgatg atcgccgcgc gcatcgtcgg
     2581 cgccggccgc acgggattct ggatctgctt cggggcgatg atcgtgtcgc tgctgatcgt
     2641 tggcttcgcg gcgcggatct tccacatcgg cggcctgctg ggcctgttcg tggcgccgct
     2701 gggctacatg tggatcctcg acaccacgta cctgcgcggc ctggcgatgg tgttcctgca
     2761 gtacctgatc gtcgccgcct tcgcggtcat cctcgcgttc acggcgctgg gctcgatgct
     2821 gcacgtcaag gacatgatgc gccacctcga caccgccccg gcgcagtcgg tctgaggcgg
     2881 cggcggcgag cgaccgcgct gccgcgccgg cccccaccac gtgtcgtcct gcgggcgctc
     2941 cgcggcgctc actcgtcgcg cgaggcgcgg cgcaccggca cctggctgtc gcgcacgttc
     3001 ttctggatcg cgatcgtcgc gaacagcgcc agcaggaagg ccatgccgta gaagcccttc
     3061 tccgacagcg cgagctgcgc gttgtacagg cccacgccca tcagcgtgat cgtgatggcc
     3121 atggacaacc agcacaggcc cgcgtacagc ccggtgacgg gcaggccctc gaggcggtcg
     3181 cgcaccgtct tctgcagcga gaccgcagcg aacaggccgt acatcagcag cgtgaagtag
     3241 tagcccttct cgttgagctg catgttggtg ttccacaggc cgaacaggta ggcgccggca
     3301 ccggcgatca gcgcggtcca ggacgcgccg acgaaggcgg cagtgggttt gttggcgttg
     3361 gcggacatcc gggttctccc ttggggtcgt tggtatgggt ggccgcccct cgcggcctgc
     3421 gcgcattctg gccgcggccg cgcgagttcg catcccgccg acgcggggat gcgccaagta
     3481 ttgccgcgcg ccccttgaaa gcgccagccg gcgctactcg cccgggtagc gcaggccgaa
     3541 ctgggcgcgc atcgcgtcca tccagccc
//
LOCUS       DATCRO010000210         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1104970, whole genome shotgun sequence.
ACCESSION   DATCRO010000210 DATCRO010000000
VERSION     DATCRO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104970"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VIN75_05700"
     CDS             <1..625
                     /locus_tag="VIN75_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY8973691.1"
                     /translation="PDFVGHALREQRAGLAQIAFNRLPLRAGHGVIAVASLAFADHAP
                     LPQIYTADGEGRSPPLQWAGVPDAATSLVLLVEDADSPTPHPLVHAIAVGLPRGDGSL
                     QTGALDGGDDTPDDGRLGRNSYLRHGWLPPDPPPGHGPHRYAFQVFALGGDEPLPDSP
                     GRDAVLELLEKRTIASGLLIGTYERHDTSVDAREPAADASPTGAAPA"
     gene            1144..>2141
                     /locus_tag="VIN75_05705"
     CDS             1144..>2141
                     /locus_tag="VIN75_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8973692.1"
                     /translation="MTRIDTPLSKPRDLRNIGVIAHVDAGKTTTTERILFYTGENHRI
                     GEVHDGNTVMDYDPQERKRGITINSAAVTVHWNGTQVNLIDTPGHIDFNIEVNRSLRV
                     LDGAVVVFDGVAGVEPQTETNWRLADKYRVPRIAFVNKLDRTGADFARVVAMMEERLG
                     VVALPLQLPIGAEGDFRGVVDLLRMRAFVWDKDDASAPPRETDVPAELLAAARHARAR
                     LVEAVVEQDDALLDAWLRGDEIPLASLQAGLRAGTLASAFVPVLAGSAFKNRGVEPLL
                     DAVVAYLPRPGETSHEDATLDADPDAPFAALAFKVVADDHGAMTFVRVYRGRLARGA"
BASE COUNT          322 a          794 c          736 g          289 t
ORIGIN      
        1 gcccgacttc gtcggccacg cgctgcgcga gcagcgcgcg ggcctggccc agatcgcctt
       61 caaccgactg ccgctgcgcg ccggccacgg cgtgatcgcg gtcgcgagcc tggcgttcgc
      121 cgaccacgcg ccgctgccgc agatctacac cgcggacggc gagggccgtt cgccgccgct
      181 gcagtgggcc ggcgtgccgg atgcggcgac gtcgctcgtg ctgctcgtcg aggacgcgga
      241 ttcgccgacg ccgcacccgc tggtgcatgc gatcgccgta ggcctgccgc gcggcgacgg
      301 ctcgctgcag acgggcgcgc tcgacggcgg cgacgacacc cccgacgacg ggcggctggg
      361 ccgcaactcc tacctgcgcc acggctggct cccgcccgac ccgccgccgg gccacggccc
      421 gcaccgctac gcgttccagg tcttcgccct gggcggcgac gagcccctgc cggactcgcc
      481 ggggcgcgac gcggtgctgg agctgctgga aaagcggacg atcgccagcg ggctgctgat
      541 tggcacctac gagcggcacg acacgtcggt ggacgcgagg gagccggccg ccgatgcatc
      601 tcccacgggc gccgcgccgg cataggcacg cgtcgcgacc gcgggcgccc accaagtgtc
      661 atcctgcgcg gtggcggttg gcggttggcc ggtggcagtg cggccttcgc aacgccttgt
      721 ccgattgaaa gccaccactg cttgtagagc catcgccctc gccggataat gacggccatc
      781 atgaaaatca ctcaccactt cctcgcccgc atgggccaag tgaacaaggc cacgctggcc
      841 acctgagtgc tcttcgacca ttcgtcgggg ggccgcgccc tgccgaccac gacgtcgacc
      901 ccggcaggcg caagtctccc ggggtttcgt ttttccgcat ccactgcaag gctcgccatg
      961 cgcacgtacg acaagctgat cgccacgatt cccgaccgcc gggacatcgc tcgcgcacgc
     1021 ccgtgccccg gccacgggcc tcacgaacac cgctgaccac ctggtgaatc ccgccgggtg
     1081 gcggctggaa cggcacacac ccggtgacgc ggcacggcgc cgcgtcccat cgaaggattc
     1141 atcatgacaa ggatcgacac gcccctcagc aaaccgcgcg acctgcgcaa catcggtgtc
     1201 atcgcgcacg tggacgcggg caagaccacg accaccgaac gcatcctgtt ctacacgggc
     1261 gagaaccatc gcatcggcga agtgcacgac ggcaacaccg tcatggacta cgacccgcag
     1321 gagcgcaagc gcggcatcac gatcaacagc gcggccgtca ccgtgcactg gaacggcacg
     1381 caggtcaacc tgatcgacac gccggggcac atcgacttca acatcgaggt caaccgctcg
     1441 ctgcgcgtgc tggacggcgc cgtcgtcgtg ttcgacggcg tggccggcgt cgagccgcag
     1501 accgagacga actggcgcct ggcggacaag taccgcgtgc cgcgcatcgc cttcgtcaac
     1561 aagctcgacc gcacgggcgc ggacttcgcg cgcgtcgtcg cgatgatgga ggagcgcctg
     1621 ggcgtggtcg cgctgcccct gcagctgccg atcggcgcgg agggcgactt ccgcggcgtc
     1681 gtcgacctgc tgcggatgcg cgcgttcgtc tgggacaagg acgacgccag cgcgccgccg
     1741 cgcgagacgg acgtgcccgc cgagcttctc gctgcggcgc ggcacgcccg tgcgcgcctg
     1801 gtcgaggccg tcgtcgaaca ggacgacgcg ctgctggacg cctggctgcg cggcgacgag
     1861 atcccgctcg cgtcgctgca ggccggcctg cgcgcgggca cgctcgcgtc ggccttcgtg
     1921 ccggtgctgg cgggctccgc cttcaagaac cgcggcgtgg agccgctgct ggacgcggtc
     1981 gtggcctatc tgccgcgccc gggagagacg agtcatgaag acgcgacact ggatgcggat
     2041 cccgacgcgc cattcgcggc cctcgcgttc aaggtcgtgg ccgacgacca tggcgcgatg
     2101 accttcgtgc gcgtgtaccg cggccgcctg gcgcgcggcg c
//
LOCUS       DATCRO010000211         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_13580, whole genome shotgun sequence.
ACCESSION   DATCRO010000211 DATCRO010000000
VERSION     DATCRO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13580"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..859
                     /locus_tag="VIN75_05710"
     CDS             308..859
                     /locus_tag="VIN75_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973693.1"
                     /translation="MLGDLCVVEWAYGYLGAQMQAPLPAWKPGTLHRAGESRPPLPVA
                     LHDARVDIQGKLGSWARLIAEEANMRGPADNTVPAIALWLRAQLHWASDQSWVDSYAA
                     ELADLRKTAYGLAPWDRARQDLPLPCPARGCGMLSLSWYSGSETVVCRSRLCGHRMTV
                     GDYVAAVMAEWERQTAAREMATT"
     gene            856..1107
                     /locus_tag="VIN75_05715"
     CDS             856..1107
                     /locus_tag="VIN75_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973694.1"
                     /translation="MTKRVDLPEGLPADLAATHHELDLDEIAARTYFKIGRHWFMAQS
                     VNLRTSTYGIPSRRDEDPVRDFDEVEMRLVHAHWRGCDD"
     gene            1179..1517
                     /locus_tag="VIN75_05720"
     CDS             1179..1517
                     /locus_tag="VIN75_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8973695.1"
                     /translation="MPTTLTDPAQKYLRLKCGMPATTMRPAALDADPDDPEQRLLTTA
                     EAAAAAHVDEARIRDWARRGLIKPVATPNGQPVYRELDVLAVEAATRRAARERALAAE
                     AMEGLGNAPA"
     gene            1654..1899
                     /locus_tag="VIN75_05725"
     CDS             1654..1899
                     /locus_tag="VIN75_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973696.1"
                     /translation="MSVIDDAKAFLSKLEADASHLYDEGKALVEKLLGEAEADVADIE
                     QEAAPVLQAAKADAEQLATDAVSGAEAVVSDSGKASE"
     gene            2008..2559
                     /locus_tag="VIN75_05730"
     CDS             2008..2559
                     /locus_tag="VIN75_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973697.1"
                     /translation="MYDPQPGDIGLTQIEGDVGRLIRLGQYLNGGGFADYEHAFVYLG
                     NGQIIEAEPGGARIAELTEYDQRTLAWLKCPPQYRDAVAEAARSQVGTPYSFADYLAI
                     ALHRFHVPAPGLRKFIASGKHAMCSALADRIAALGGWHWYDDGRWEGYVTPADVWRLI
                     QAQNAATTPPPVPHRPIVPGMGV"
     gene            2633..>3037
                     /locus_tag="VIN75_05735"
     CDS             2633..>3037
                     /locus_tag="VIN75_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973698.1"
                     /translation="MTGIVVQNDPINPGQEPYARGPVHIELVTGTSGGAGYTGTQSII
                     GTWTTETDAAGNWSATLTPTNAITPANTHYRVNEGGFVSLIQVPDSGGPYNLSQLLVT
                     NPPTPSAPGITGEQIAANGVVAGVRPEVNFIGG"
BASE COUNT          492 a         1088 c         1044 g          413 t
ORIGIN      
        1 catgcgcgcc cacatctggc agcaccaccc cgacgagacg tggccgccag ccgagcccga
       61 gtggctcatg ccccgaccgg acgacgaggc cggtgatccc gcgtgacgca cttcgcgtgg
      121 accatgctca cggtcctgat cacagcggct gcagtcaacc agagcttcga ggcgctgggc
      181 cggatcgggc gactgctggc ccggctgacg cgatcggggg gcggggatgg ctgacttcgc
      241 gtgtgtcgtt tgtggcctgc gcccttccct cggtcgcagc tacgtggacg gcggctgctt
      301 cgcccgcatg ctgggcgacc tgtgcgtcgt cgaatgggct tacggctacc tcggcgcgca
      361 gatgcaggcc ccgctccccg cgtggaagcc gggtaccctg caccgcgccg gcgagtcccg
      421 tccgccgctg ccggtcgcgc tgcacgacgc gcgcgtcgac atccaaggca aactcggctc
      481 gtgggcgcgg ctgatcgcgg aagaggcgaa catgcgcggc ccggcggaca acaccgtccc
      541 cgcgatcgcg ctgtggctgc gcgcgcaact gcactgggcg tcggatcagt cgtgggtcga
      601 ctcgtacgcc gccgagctgg ccgacctgcg caagaccgcg tacgggctcg caccctggga
      661 tcgggcgcgc caagatttgc ccttaccctg tccggcgcgc ggatgcggca tgctcagctt
      721 gagctggtac agcggcagcg agacggtggt gtgccgcagc aggctgtgtg gtcaccggat
      781 gacggtcggc gactacgtgg cggcggtgat ggcggaatgg gaacggcaga cggcggcgcg
      841 ggagatggcg acgacatgac caaacgtgtg gacttacccg aagggctgcc tgctgatctg
      901 gcggcaactc accacgaact cgacctcgac gagatcgcgg cgcgcacgta cttcaagatc
      961 gggcggcact ggttcatggc gcagtctgtg aatctgcgca ccagcaccta cgggattccg
     1021 tcgcgccgcg acgaggaccc tgtccgtgac ttcgacgagg tcgagatgag gctcgtgcac
     1081 gcccactggc gcggctgcga tgactgagcc cggtccgaac tctgtcgccg ccagcaggta
     1141 tccttccggc atcccccgag acgatcagga cgccgacggt gcccaccacc ctcaccgacc
     1201 cggcgcagaa gtacctgcgc ctcaagtgcg gcatgcccgc gaccacgatg cgcccggccg
     1261 cgcttgacgc cgaccccgac gatccggagc agcggctgct gaccacggcc gaagccgcgg
     1321 cggcggcgca cgtggacgaa gcccggatac gcgactgggc gcggcgcggg ctgatcaagc
     1381 ccgtcgcgac gccgaacggg cagccggtct accgcgaact cgacgtgctc gccgttgaag
     1441 ccgccacgcg ccgtgccgca cgcgaacgag cgctcgcggc cgaagcgatg gaagggctcg
     1501 gcaacgcgcc cgcttgacgc gggcctggag tttcgcacat catctgcacg tcggcgtatc
     1561 acgccctgaa gccccccgga cctgcggtct tgtgggggct ttcgcctttt ctggcccgat
     1621 tcggccaccc cactcagacc cgggagtacc gccatgtccg tgatcgacga cgccaaggcc
     1681 ttcctgtcca agctggaggc cgacgccagc cacctgtacg acgagggcaa ggccctggtc
     1741 gagaagctgc tcggcgaggc cgaggccgac gtcgccgaca tcgagcagga agccgccccc
     1801 gtcctccagg ccgcgaaggc ggacgccgag cagctggcca ccgacgccgt cagcggcgcc
     1861 gaggccgtcg tgtccgacag cggcaaggcg agtgagtgag cgtcggctac tgctgcgccg
     1921 tgttcgtcga gcacgacttc gagaccgggc gcaccgacac ctcgcgctgc caggttcacc
     1981 gctgctgccc catccacgga ggccgcgatg tacgacccgc agcccggtga catcggcctc
     2041 acgcagatcg aaggcgatgt cgggcgcctg atcaggctcg ggcagtacct taacggcggc
     2101 ggcttcgccg actacgagca cgcgttcgtg tacctcggca atgggcagat catcgaggcc
     2161 gagcctggcg gcgcgcgcat cgccgagctg accgagtacg accagcgcac tctcgcgtgg
     2221 ctcaagtgcc cgccgcaata ccgcgatgcc gtcgccgagg cggcgcgtag ccaggtcggc
     2281 acgccctaca gcttcgccga ctacctggcg atcgccctgc accgcttcca cgtcccggcg
     2341 ccgggcctgc gcaagttcat cgcgtcaggc aagcacgcca tgtgcagcgc tctggcggac
     2401 cgcatcgccg cgctcggcgg ctggcactgg tatgacgacg gccggtggga gggctacgtg
     2461 acgccagcgg acgtctggcg gcttatccag gcgcagaacg cagccaccac gccgccaccg
     2521 gtcccgcatc ggccgatcgt gccgggcatg ggcgtgtagc cctccacgaa ctggccgccc
     2581 ttgaccctgc cggcccgggg cggccggacc accacggcgc acggaggcgg cgatgaccgg
     2641 gatcgtcgtc cagaacgacc cgatcaaccc gggccaagag ccgtacgcgc gcggcccggt
     2701 gcacatcgag ctggtcactg gcacgagcgg cggggcgggc tacaccggga cgcagagcat
     2761 catcggcacc tggaccacgg agaccgatgc ggccgggaac tggtccgcga cgctcacgcc
     2821 gaccaacgcg atcaccccgg cgaacacgca ctaccgggtg aacgagggcg gcttcgtcag
     2881 cctgatccag gtgccggact cgggcggccc gtacaacttg agccagctgc tcgtcaccaa
     2941 cccgccgaca ccgagcgccc caggcatcac cggcgagcag atcgcggcga acggcgtcgt
     3001 cgctggcgtg aggcccgagg tcaacttcat cggcggg
//
LOCUS       DATCRO010000212         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_913650, whole genome shotgun sequence.
ACCESSION   DATCRO010000212 DATCRO010000000
VERSION     DATCRO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913650"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VIN75_05740"
     CDS             complement(<1..979)
                     /locus_tag="VIN75_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973699.1"
                     /translation="MKAWPVPGPRGIGAKLTLAFGALAGVTFVVVALAWGAGQRVTHD
                     IDLSELVRRPASLASAQAQADLLRMQLHVRGYLVLSDPADIEQYRAARKSFEAEMAAL
                     QSMSNQWPEAEDARAVRALVEDYRAWAQLPQQLFELHDSPFKNRPALRLARVEVQARL
                     VRILDAIDGIAERQKRREGDARSRELLGDLLNFQTSFDAMATNLMAFGASGEVNFKLT
                     YGPQLATNAAAWRALSARRGFLDERQRELLEVIARNRAEVADIGLQIVSIVNGEHAYE
                     DLYLYRTQVAPQADALLRRLADLAARQQAQLGTSLAHARTSLSSVKVVAA"
     gene            complement(976..2559)
                     /locus_tag="VIN75_05745"
     CDS             complement(976..2559)
                     /locus_tag="VIN75_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8973700.1"
                     /translation="MQSTLKWMGVLVSAAALAASAGEARTPEVENYKASVVKQGPIVP
                     PRVAPPCVPGRCPFAGQTVTVLMVKERNGGALGELKDEFEAATGAKLDLVQLEHQDLF
                     PNFMSDLTNGVGKYDAAYAGAWWLGELVSGGYLLSYDKFYGDPRFPQWTIDDVLPGPR
                     RLLSYAGNKYMVANDHDGQVMYYRRDLLEAPAHRAAFQAKYGYPLGVPATWAQFRDVA
                     EYFDGKDLGGDGVPGHGLTMHLKVGAQGMFHFMSFSAPFVIGPDNPDLYWFDPRTMKP
                     LIESPGHVRALKALVDLVKFGPRDMLNWDLGRSWDYFLAGHAALTFTWGDLGALAQDE
                     GSRVKGKIGSAPMPGTDEYYSLASKRWIRTSRPNVVGNTTGGSWAGVISRYSKSPEAT
                     YFLFALMATREKSKVYAVRGWDGIDPGRVFHFLPPDGTGDLQTYLRAGWDEADVRPYL
                     HAYFENFSNPLQFPYLRIPGAFGYWQALDVHLAEAVAGQSTPEAALRATAVDFEEITI
                     RLKRDKQLRSYRASLGLKQ"
     gene            complement(2590..3744)
                     /locus_tag="VIN75_05750"
     CDS             complement(2590..3744)
                     /locus_tag="VIN75_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973701.1"
                     /translation="MDAPAQPASGGTILIVDDMPANLEVVTSHLERQGYRAVVALSGD
                     EGIERAEYVQPDLILLDIMMPGIDGFEICRRLKAGDRTRAIPVIFMTALSDTTDKLAG
                     FAAGAVDYVTKPLNGAEVLARIESHLALYALRRQLVAQNAQLQQEIAVRAEAEAALQR
                     SNTEFEQLAYVASHDMQEPLRKIASYLQLLSQRYQGQLDADADEFIGYAVDGAKRMQA
                     LINDLLAFSRVGTKAKPFAPADCGLIVKTALADLQFAIEDAGARVDVGDLPMVMGDAT
                     QLSQLFRNLISNAIKFRRDEPPVVRVTSERSEAFWRFTVSDNGIGIAAEYFERIFVIF
                     QRLHGRSQYAGTGIGLAICKKIVERHGGQIEVRSVEGEGSEFAFTLPALQ"
     gene            3894..>4585
                     /locus_tag="VIN75_05755"
     CDS             3894..>4585
                     /locus_tag="VIN75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY8973702.1"
                     /translation="MPAPCGPRRALLPRRPATPRRTACALCAAFGAACQADEAPLQQV
                     EIVGIRASADAAAMLKKQRDEVSDAIVADDIDKLPDASVAEALQRVPGVQIARDRGEG
                     ATVVVRGLGQVETTVDGREVFSAGTGRTLDFASMASALVAGVDVYKTPSAERIEGGMA
                     GQVDLRTRRPFDFRHGALEVTARALRAPLAAGSAIQFSGVASTRLPLAGGELGLLAAI
                     AVEPRPWREDQK"
BASE COUNT          686 a         1600 c         1581 g          718 t
ORIGIN      
        1 cggccgccac caccttgacc gacgacaggc tcgtgcgggc atgcgcgagg ctggtcccga
       61 gctgcgcctg ctggcgggcg gccaggtcgg ccaggcgccg cagaagcgcg tccgcctgcg
      121 gcgcgacctg tgtccgatac aggtacaggt cttcgtacgc gtgctcgcca ttgacgatcg
      181 acacgatctg caagccgatg tccgcgacct cggcccggtt gcgcgcgatg acctccagca
      241 gctcgcgctg gcgctcgtcg aggaagccgc gcctggccga caaggccctc caggcggcgg
      301 cattggtcgc gagctgcggg ccgtaggtca gcttgaaatt cacttcgccc gacgcgccga
      361 aggccatcag gttcgtggcc atcgcgtcga acgaggtctg gaagttcagc aggtccccga
      421 gcagttcgcg gctgcgcgca tcgccttcgc gccgcttctg ccgctccgcg atgccgtcga
      481 tcgcgtcgag gatgcggacc agccgggcct gcacctccac ccgggccagc cggagcgcgg
      541 gccggttctt gaagggactg tcgtgcagct cgaacagctg ctgcggaagc tgcgcccagg
      601 cacggtagtc ctccacgagc gcgcgcacgg cgcgcgcgtc ctcggcctcc ggccactggt
      661 tcgacatcga ttgcagcgcc gccatctcgg cctcgaacga cttgcgcgcg gcgcggtact
      721 gctcgatgtc cgccggatcg ctgagcacca ggtagccgcg cacgtgcaac tgcatcctga
      781 gcaggtcggc ctgcgcctgc gcggacgcga gcgacgcggg ccggcgcacc agttcgctca
      841 ggtcgatgtc gtgggtcacg cgttgacccg ccccccacgc cagcgcgacg accacgaagg
      901 tcacgccggc cagggccccg aacgccagcg tgagcttggc gccgatcccc cgcggcccgg
      961 ggactggcca tgccttcatt gcttcaggcc gagcgaggcc cggtaggagc gcagctgctt
     1021 gtcgcgcttg aggcggatcg tgatctcctc gaagtcgacg gcggtcgcgc gcagcgcggc
     1081 ctcgggcgtc gactgccccg cgacggcttc cgccagatgg acgtcgagcg cctgccagta
     1141 gccgaaggcg ccgggaatgc gcaggtacgg gaactgcagc gggttgctga agttctcgaa
     1201 gtacgcatgc aggtatggac gcacatcggc ctcgtcccag cctgcacgca ggtaggtctg
     1261 caggtcgccc gtgccgtccg gcggcaggaa gtggaagacg cggcccggat cgatcccgtc
     1321 ccagccgcgc acggcataga ccttggactt ctccctggtc gccatcaacg cgaacaggaa
     1381 gtacgtggcc tccggcgact tcgagtacct ggagatgacg ccggcccatg atccgccagt
     1441 ggtgttgccc acgacgttgg gccggctcgt cctgatccag cgcttgctcg cgaggctgta
     1501 gtactcgtcg gttcccggca tcggcgcact gccgatcttg cccttgaccc ggctgccctc
     1561 gtcctgcgcc agggcaccga ggtcgcccca ggtgaacgtc agcgccgcat ggccggcgag
     1621 gaagtagtcc cagctcctgc ccaggtccca gttgagcatg tcgcgcggcc cgaacttcac
     1681 gaggtcgacc agcgccttga gcgcccgcac atggcccggg ctctcgatca acggcttcat
     1741 cgtgcgcggg tcgaaccagt agaggtcggg gttgtccggg ccgatcacga acggcgccga
     1801 gaacgacatg aaatggaaca tgccttgggc accgaccttc aggtgcatgg tcaggccatg
     1861 gccgggcacg ccgtcgccgc cgagatcctt gccgtcgaag tattcggcca cgtcgcggaa
     1921 ctgcgcccag gtcgcgggca cccccagcgg atagccgtac ttcgcctgga acgcggcgcg
     1981 gtgggccggc gcctcgagga gatcgcgccg gtagtacatc acctggccgt cgtggtcgtt
     2041 ggcgaccatg tacttgttgc cggcatagct cagcaggcga cgggggcccg gcagcacgtc
     2101 gtcgatggtc cattgcggga agcgcgggtc gccatagaac ttgtcgtagg acagcaggta
     2161 gccgcccgac acgagctcac ccagccacca tgcgcccgcg taggccgcgt cgtatttccc
     2221 gacgccgttc gtgaggtcgg acatgaagtt ggggaacagg tcctggtgct cgagctggac
     2281 gagatcaagc ttcgcgccgg tggcggcctc gaactcgtcc ttgagctcgc cgagcgcgcc
     2341 accgttcctt tccttgacca tcagcaccgt caccgtctgt ccggcgaagg ggcatcggcc
     2401 cggcacgcaa ggcggcgcga cgcgcggcgg cacgatgggg ccctgcttga cgaccgaggc
     2461 cttgtagttc tcgacctcgg gcgtcctggc ctcccctgcc gaggcggcca acgcggccgc
     2521 ggagaccagg actcccatcc atttcagcgt gctttgcatg gcgacacctc ctgaaaaggc
     2581 ctcgaggatt cactgcaggg ccggcaacgt gaaggcgaac tccgatccct cgccctcgac
     2641 cgatcgcacc tcgatctgcc cgccatgccg ttcgacgatc ttcttgcaga tggccaggcc
     2701 gatgcccgtg cccgcgtact ggctgcgccc gtgaaggcgc tggaagatga cgaagatgcg
     2761 ctcgaaatat tcggcggcga tgccgatgcc gttgtcgctg acggtgaagc gccagaatgc
     2821 ttcggaacgc tccgacgtca cgcgcacgac cggcggttcg tcgcgacgga acttgatggc
     2881 gttggagatc aggttgcgaa acagctggga caactgcgtc gcgtcgccca tcaccatcgg
     2941 caggtcgccc acgtcgacgc gcgcgccggc gtcctcgatc gcgaattgca ggtcggccag
     3001 ggccgtcttg acgatcagcc cgcaatcggc gggggcgaac ggcttggcct tggtccccac
     3061 ccgcgagaac gccagcaggt cgttgatcag cgcctgcatg cgcttggcgc cgtcgaccgc
     3121 gtagccgatg aactcgtcgg cgtcggcgtc cagctggccc tggtagcgct gggacagcag
     3181 ttgcaggtag ctggcgatct tgcgcagggg ctcctgcatg tcgtgcgacg cgacataggc
     3241 cagctgttcg aactccgtgt tggaccgctg gagcgccgcc tccgcttccg cccgcacggc
     3301 gatctcctgc tgcaactggg cgttctgcgc caccagttgc cggcgcaggg catagagcgc
     3361 caggtggctt tcgatgcgcg ccagcacctc ggcgccgttg aggggcttgg tgacgtagtc
     3421 gacggcgccg gcggcgaacc cggccagctt gtcggtggta tcggacagcg cggtcatgaa
     3481 gatcaccggg atggcgcgcg tgcgatcgcc ggccttcagg cgccggcaga tctcgaagcc
     3541 gtcgatgccg ggcatcatga tgtccagcag gatcaggtcc ggctgcacgt actcggcccg
     3601 ttcgatgccc tcgtccccgc tcagggccac cacggcccga tagccctggc gctccaggtg
     3661 gctggtcacc acttccaggt tggcgggcat gtcgtcgacg atcaggatcg tcccacccga
     3721 tgccggttgc gcaggagcgt ccatctcacg tccctcggtc gtcctggcac gagtctatgc
     3781 cgcaccgcgg acatccgcca tagacggacc gcgcctgggt gaccgggccg cgaggttccg
     3841 gcgcccctgg ccacgggctg gcgcaggaag gccgattgcg cttcaatgcg ggcatgccag
     3901 cgccctgcgg accgcgacgc gccctcctgc cccggcgacc tgcgacgccg cgcaggacgg
     3961 cgtgcgcgct gtgcgcggcg ttcggcgccg cgtgccaggc cgacgaggcg ccgctgcagc
     4021 aggtcgagat cgtgggcatc cgtgccagcg ccgatgccgc cgccatgctc aagaagcagc
     4081 gcgacgaggt cagcgacgcc atcgtcgccg acgacatcga caagctgccg gacgcgagcg
     4141 tcgccgaggc gctgcagcgc gtgcctggcg tgcagatcgc gcgcgaccgg ggcgaaggcg
     4201 cgaccgtggt cgtccgcggc ctcggccagg tggagacgac cgtcgacggg cgcgaagtct
     4261 tcagcgccgg cacgggccgg acgctcgact tcgcgtcgat ggcctcggcg ctggtcgcgg
     4321 gcgtggacgt ctacaagacg ccctcggccg aacggatcga gggcggcatg gccggccagg
     4381 tcgacctgcg cacgcgccgg ccgttcgatt tccggcacgg cgccctcgag gtcaccgctc
     4441 gtgcgctgcg tgcgcccctg gccgcgggca gcgcgatcca gttctccggc gtggcctcga
     4501 cccgactgcc gctggccggc ggcgaactcg gcctgctggc cgcgatcgcc gtggagccgc
     4561 gcccctggcg cgaggaccag aagag
//
LOCUS       DATCRO010000213         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_610038, whole genome shotgun sequence.
ACCESSION   DATCRO010000213 DATCRO010000000
VERSION     DATCRO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610038"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VIN75_05760"
     CDS             complement(<1..785)
                     /locus_tag="VIN75_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY8973703.1"
                     /translation="MNAPLPDSIRKALETVSLDDKYALDTGRAFMSGVQALVRLPMLQ
                     RTRDALAGLNTAGFISGYRGSPLGGYDQALWAAKKHLAAQNVVFQPGVNEELGATAVW
                     GTQQLALYPQTSKFDGVFGLWYGKGPGVDRTADVFKHANMAGTSAHGGVIAIAGDDHV
                     SKSSTAAHQSDHIFKACGIPVFFPSSVQDILDMGLHAFAMSRFSGVWTSMKTIQEVVE
                     SSATVDVDPHRVDIVLPEDFVMPPGGVHIRWPDPPLEQEARLM"
     gene            1009..1581
                     /locus_tag="VIN75_05765"
     CDS             1009..1581
                     /locus_tag="VIN75_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8973704.1"
                     /translation="MPRSEKTGKANARASEPARAREADERGARDAGGLDRYDIALLGE
                     LQRDARQSNADLAARIGLSPAPTWRRVRRLEELGVITGYRAEIDRRKIGLGVLAFVRV
                     DTDRDNLAATKALEDAIRGLPEVISCHYISGAGTFELQVLVTDLDAYARWTMDTLFRL
                     PNVKDLHTSFSLGEVKAGAALPLGHLGAAR"
     gene            1895..3406
                     /locus_tag="VIN75_05770"
     CDS             1895..3406
                     /locus_tag="VIN75_05770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8973705.1"
                     /translation="MNRRDVLLRTGTALAGLAGASARAADAPDALLTAEEQAWVARHP
                     VVLFAPERDFPPFSFVDSQGQHRGLSADVLDLVQEHTGLKFQAASAGERALNIDRLKR
                     REVDLLTSLRPTADREQFISFTSAYVSSPAVVLRRRNDHRSGDLAKMAGERAAVDRNS
                     AAEDFLRRTWPDVVAVEVETAAQGLRELAFGEVDACVVNLATASYLIERDRIGGLRVA
                     GETGYFSTLTLGYRKDWPMLGRVLEKGLAQISDAERAQMIARWIPLSDIAWWQRPEVQ
                     RVLGAASIGTGLLMGGLLLWNRALRRAVSQRTDALQKELAERQRLETRLRTLAEHDPL
                     TGLMNRAALTEALRRSLALAARQKWSVAVVFIDLDKFKAVNDSMGHAAGDELLRQIAS
                     RLQGCLRESDLLGRLGGDEFIVVAEALHDGPRNAMELADKLLMQMKRPFPVEGRSLAM
                     GFSAGIALFPGDGDTPESLIANADAAMYQAKEQGRFRASLYRGSAPVSGEETA"
     gene            3403..>3585
                     /locus_tag="VIN75_05775"
     CDS             3403..>3585
                     /locus_tag="VIN75_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973706.1"
                     /translation="MKRPACLALAALALAASLPARAAEPVTSGFHAAPPASDAAPARA
                     RRVTRASVPAANFLVEW"
BASE COUNT          535 a         1229 c         1295 g          526 t
ORIGIN      
        1 tccatcagcc gcgcttcctg ctccagcggc ggatcgggcc agcggatgtg cacgccgccc
       61 ggcggcatca cgaagtcctc gggcagcacg atgtcgacgc gatgcgggtc gacgtcgacg
      121 gtggccgacg attcgaccac ctcctggatc gtcttcatcg acgtccacac gcccgagaag
      181 cggctcatcg cgaacgcgtg caggcccatg tccaggatgt cctgcaccga cgacgggaag
      241 aacaccggga tgccgcaggc cttgaagatg tggtcgctct ggtgggcggc ggtgctgctc
      301 ttggagacgt ggtcgtcgcc ggcgatggcg atgacgccgc cgtgtgccga ggtgccggcc
      361 atgttggcgt gcttgaacac gtcggcggtg cggtcgacgc ccgggccctt gccgtaccag
      421 aggccgaaca cgccgtcgaa cttgctcgtc tgcgggtaga gcgcgagctg ctgcgtgccc
      481 cagacggcgg tcgcgcccag ttcctcgttg actcccggct ggaacacgac gttctgcgcg
      541 gccaggtgct tcttggcggc ccacagcgcc tggtcgtagc cgcccagcgg ggagccccgg
      601 tagccgctga tgaagccggc ggtgttcagg ccggcgagcg cgtcgcgcgt gcgctggagc
      661 atgggcaggc gcacgagggc ctgcacgccg ctcatgaacg cacggccggt gtcgagcgcg
      721 tacttgtcgt ccagcgacac ggtctcgagg gctttgcgga tgctgtcggg caacggggcg
      781 ttcatgggcg cgtctcctgc gggtcaggac gtcaaggatg gggacgggtg gcgcgttgcc
      841 gcggcgtggg cgacgacgtt ccggtgcggc tcgtgaccgg ttggaacgat gggcaacgaa
      901 cggcaatcag tgtagggaaa agccgtggac atgtgcttgc tttatgcttg tcctggcgcg
      961 gccgttgaga aacaatattg ccgcgaaacc gatatcggag aaccactctt gccaagaagc
     1021 gagaagacgg gaaaagcgaa cgcccgtgcg agcgagccgg cccgcgcgcg cgaggccgac
     1081 gagcgcggag cgcgggacgc cggcggcctc gaccgctacg acatcgcgct gctgggcgaa
     1141 ctgcagcgcg acgcgcgcca gtccaacgcc gacctggccg cgcgcatcgg cctgtctccc
     1201 gcgcccacct ggcgccgcgt gcgccggctc gaggaactgg gcgtcatcac cggctaccgc
     1261 gccgagatcg accgccgcaa gatcggcctg ggcgtgctcg ccttcgtgcg cgtggacacc
     1321 gaccgcgaca acctcgccgc caccaaggcg ctggaagacg cgatccgcgg actgccggag
     1381 gtcatctcct gccactacat cagcggcgcc ggcacgttcg agctgcaggt gctggtgacc
     1441 gacctggacg cctatgcgcg ctggacgatg gacacgctgt tccgcctgcc caacgtgaag
     1501 gacctgcaca ccagcttctc gctgggcgag gtcaaggccg gcgccgcgct gccgctgggg
     1561 catctcgggg cggcacgatg aggtgcaagc gatgttgggc gacggacggg ttaatccggt
     1621 gcacggcacc ttcgtttgcg gagagcatca aatgtgtcgt catcggaaag ttctctgaag
     1681 aatcccctcg aaaacgcccg tggccgtcgt tgccgtgctc gcgaggcatc gagcctcgct
     1741 ccgcccggcg cctgggccac agccgtttgc gagaggattc ggccacattg gcgctgcgat
     1801 cgagaccctt tcaatgaact tccaaatcac gacactggaa gggtcgacct cgaacgggca
     1861 gcgcagcccg tcgtcacaac gaactgggca cgcattgaac cgacgtgatg tcctcttgcg
     1921 aacgggcacg gccctggcgg gcctcgcggg cgcatccgcg cgcgccgcgg acgcgcccga
     1981 cgcgctgctg accgccgagg aacaggcgtg ggtcgccagg cacccggtgg tgctgttcgc
     2041 gccggagcgc gacttcccgc cgttcagctt cgtcgacagc cagggccagc accgcgggtt
     2101 gtcggccgac gtgctggacc tggtgcagga acacaccggc ctgaagttcc aggctgcgtc
     2161 cgccggcgag cgcgcgctca acatcgaccg cctgaagcgg cgcgaggtcg acctgctcac
     2221 gtcgctgcgc cccacggccg accgcgagca gttcatctcc ttcacgagtg cctacgtcag
     2281 ctcgcccgcg gtggtgctgc ggcgtcgcaa cgaccaccgc tcgggcgacc tggcaaagat
     2341 ggccggcgag cgcgccgcgg tcgaccgcaa ctccgccgcc gaggacttcc tgcggcgcac
     2401 ctggcccgac gtcgtggcgg tggaagtgga gacggccgcg caagggctgc gcgagctggc
     2461 cttcggcgag gtcgacgcgt gcgtcgtcaa cctggccacg gcgtcgtacc tgatcgagcg
     2521 cgaccgcatc ggcgggctgc gggtggcggg cgagacgggc tatttctcga cgctgacgct
     2581 gggctaccgc aaggactggc cgatgctggg ccgcgtgctg gagaagggcc tggcgcagat
     2641 cagcgatgcc gagcgggcgc agatgatcgc gcgctggatc ccgctgtcgg acatcgcgtg
     2701 gtggcagcgc cccgaggtgc agcgcgtgct gggcgccgcg tcgatcggca ccggcctgct
     2761 gatgggcggc ctgctgctgt ggaaccgcgc gctgcggcgc gccgtgtcgc agcgcaccga
     2821 tgcgctgcag aaggagctcg cggagcgcca gcggctggag acgcggctgc gcacgctggc
     2881 cgagcacgat ccgctcaccg gcctgatgaa ccgcgcggcg ctcaccgagg cgctgcggcg
     2941 ctcgctggcg ctggccgcgc ggcagaagtg gtcggtggcc gtcgtcttca tcgacctgga
     3001 caagttcaag gccgtcaacg attcgatggg ccacgccgcc ggcgacgagc tgctgcgcca
     3061 gatcgcctcg cggctgcagg gctgcctgcg cgagagcgac ctgctggggc gcctgggcgg
     3121 cgacgagttc atcgtggtgg ccgaggcgct gcacgacggc ccgcgcaacg cgatggagct
     3181 ggccgacaag ctgctgatgc agatgaagcg cccgttcccg gtcgagggcc ggtcgctcgc
     3241 gatgggcttc tcggccggca tcgccctgtt ccccggcgac ggcgacacgc ccgagtcgct
     3301 gatcgccaac gccgatgccg cgatgtacca ggccaaggag caggggcgct tccgggccag
     3361 cctgtaccgg ggcagcgcgc ccgtcagcgg cgaggagacc gcatgaaacg acctgcctgc
     3421 ctcgcgctgg ccgccttggc gctcgccgcg agcctgccgg cccgcgcggc cgaaccggtg
     3481 acctccggct tccacgccgc gccgccggcc tcggacgcgg cgcccgcccg cgcgcgccgc
     3541 gtcacgcgcg cgtccgtgcc tgcggccaac ttcctcgtcg aatgg
//
LOCUS       DATCRO010000214         4932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_249743, whole genome shotgun sequence.
ACCESSION   DATCRO010000214 DATCRO010000000
VERSION     DATCRO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249743"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..772)
                     /locus_tag="VIN75_05780"
     CDS             complement(104..772)
                     /locus_tag="VIN75_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973707.1"
                     /translation="MTHPLLARLRDAARQNAVPAAVLQALTLALLVAYYRWAPAHELL
                     ERLLRLKLAWGAAYSFVAGALFAGLLPRLVLRWIGRDTGRFPVELAFALAFWGWRSVE
                     VDLMYRAQAWYFGAAADWPTVLAKTAVDQLLYSPLWAVPEIALAFEWKEAGFSWRRMR
                     SRLDRDFFTVRLPAAIVGNAMVWLPAVPAIYFLPTALQLPVSNLVGSFWVLLMIVLLK
                     RQGD"
     gene            996..3008
                     /locus_tag="VIN75_05785"
     CDS             996..3008
                     /locus_tag="VIN75_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEY8973708.1"
                     /translation="MHSSPFHFTSAAPALSAEIELLAPAKNADIGIEAVNHGADAIYI
                     GGPAFGARASAGNDIRDIERLTRHAHRYGAKVFITLNTILRDDELEDARKMAWAVHEA
                     GADALIVQDMGLLMLDLPPIQLHASTQTDIRTPEKARFLQDVGFSQMVLARELDLREI
                     RAIAAVTPNATLEFFIHGALCVAYSGQCYLSHAHTGRSANRGDCSQPCRLPYTVTDAS
                     GGIVAHDKHVLSMKDNNQTANLDTLVDAGIRSFKIEGRYKDMGYVKNITAHYRRQIDE
                     MLERRPELRASSAGKAIFGFTPDPDRNFNRESTDYFVNGRQMDIGAFDTPKHAGLAIG
                     YVTKVAADHFEAELDDPAAQLNNGDGLTWLDLHKELVGMQANVVARLPGAANRWRVVP
                     NQPMSELKDIRSKPRLHRNRDMQWDRLLERKSADRLIGTWMTLSDIEGGLRLSVTDER
                     GFAAHADALLELQPARDVERGMAALREHLSKVGGTIFSLNELKIATSQPWFVPASRLN
                     ALRREALERLEQVRLDQREILPRALPVEPPVPFPEDTISYLANVYNEPAHAFYVKHGV
                     KVVEAAYESHEELGEVSLMITKHCVRWSMSLCPKQAKGVTGVQGTVRAEPLVLQNGSE
                     KLTLRFDCKPCEMHVVGKIKKQVINEAMAVPMTFFRTRPAPQRAGN"
     gene            complement(3012..3434)
                     /locus_tag="VIN75_05790"
     CDS             complement(3012..3434)
                     /locus_tag="VIN75_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973709.1"
                     /translation="MSYDFMLMRLAPTPRPGKLTELVEESAHQPIADAGPLHAAVRAS
                     PLFAPGAIEFDDEGRFTWNTPDGGQVGVSAIADVISLRMHAGWEFAAQLFELARGIWP
                     DLMLLDLQQDLVHDPASLRACIARGGQGRAPRGAEGDG"
     gene            complement(3444..3851)
                     /locus_tag="VIN75_05795"
     CDS             complement(3444..3851)
                     /locus_tag="VIN75_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973710.1"
                     /translation="MDVLTRLAALIIAILLAIDAFSAERSETLRVERHSRSARWHRED
                     DYRLHFAGGRVDSCKVGWPAWSALRDGDEVVVDTSRVFKSCDGIRRADEAGARTGLRQ
                     WLMLLPIALLLAAAFGWIQFERDLRAERRWWFG"
     gene            complement(3916..4119)
                     /locus_tag="VIN75_05800"
     CDS             complement(3916..4119)
                     /locus_tag="VIN75_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973711.1"
                     /translation="MLFPEHPKPVSRRRAVAEMTVAARQAAAITPGHVLVLPATAASP
                     PAPVQSTGADDPPVSPRRHDTPI"
     gene            complement(4233..4859)
                     /locus_tag="VIN75_05805"
     CDS             complement(4233..4859)
                     /locus_tag="VIN75_05805"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY8973712.1"
                     /translation="MTDSTTWPPALPLVAILRGLQPERALDVARVLFDAGFRALEVPL
                     NRPGAVAAIAAIVPQAPADACIGAGTVLETAQVDAVGDTGATLIVSPHFDAAVVSRAR
                     ERGMRTVPGVFTASEAFAAWRAGADALKIFPAEAMTHAGLAGLTTVLPPGLPLWPVGG
                     IVPGNVALWRRAGATGFGLGGGLFKPEFSVAEIASRARAYVDAWHASA"
BASE COUNT          783 a         1816 c         1726 g          607 t
ORIGIN      
        1 ggtcacgcgc ctgggcggac gcgtcggatc gacgcagatg cggctggtca acgaggaagg
       61 cgtgctgatc gccactgggg cggcgtcgta catcctcgcg tgatcagtcg ccctgcctct
      121 tgagcagcac gatcatcagc agcacccaga agctgccgac caggttggac accggcagct
      181 gcagcgccgt cggcaggaag tagatggccg gcaccgccgg cagccacacc atcgcgttgc
      241 cgacgatggc cgcgggcagg cgcaccgtga agaagtcgcg gtccagccgc gaacgcatgc
      301 gccgccacga gaagccggct tccttccact cgaaggccag cgcgatctcc ggcacggccc
      361 acagcgggct gtagagcagc tggtcgacgg ccgtcttggc cagcacggtc ggccagtctg
      421 ccgcggcgcc gaaataccag gcctgggcgc ggtacatcag gtcgacctcg acgctgcgcc
      481 agccccagaa cgccagcgcg aaggccagct cgaccgggaa gcggccggtg tcgcggccta
      541 tccagcgcag gaccaggcgc ggcagcagcc cggcgaacag cgcgccggcc acgaacgagt
      601 aggcggcgcc ccaggccagc ttcaggcgca gcaggcgctc gagcaactcg tgcgccggcg
      661 cccagcggta gtacgccacc agcaatgcca gggtcagcgc ctgcagcacg gcggcgggga
      721 cggcgttctg gcgcgcggcg tcgcgcaggc gggcgaggag cggatgggtc acggccgggc
      781 agtctatgcc ggcagcgatg acggccgggt gtcgcgaccg ggcgcgcagg tgcgcaaatc
      841 cgcccttcct gcgataatcc gccattcggc gcgccaccgg cctcccggcc ccctgaagcg
      901 cgcatcgccg ggccctccgc ccgccatcgc cgcgccccgc gcccgccgcc cacgaggcgt
      961 cgcccgcgcc cttcgagcca gaagccagca agaccatgca ttcgtccccg ttccacttca
     1021 cctcggccgc cccggccctc tcggccgaga tcgagctgct cgcgcccgcg aagaacgccg
     1081 acatcggcat cgaggcggtg aaccacggcg cggacgccat ctacatcggc ggcccggcgt
     1141 tcggcgcgcg cgcctcggcc ggcaacgaca tccgcgacat cgagcgcctc acgcgccacg
     1201 cccaccgcta cggcgccaag gtgttcatca cgctcaacac gatcctgcgc gacgacgagc
     1261 tggaggacgc gcgcaagatg gcctgggccg tgcacgaggc cggcgccgac gcgctgatcg
     1321 tgcaggacat gggtctgctc atgctcgacc tgccgccgat ccagctgcac gccagcacgc
     1381 agaccgacat ccgcacgccc gagaaggcgc gcttcctgca ggacgtcggc ttctcgcaga
     1441 tggtgctcgc gcgcgagctc gacctgcgcg agatccgcgc catcgccgcc gtcacgccca
     1501 acgccacgct ggagttcttc atccacggcg cgctgtgcgt tgcctacagc ggccagtgct
     1561 acctgagcca cgcgcacacg ggccgcagcg ccaaccgcgg cgactgctcg cagccctgcc
     1621 ggctgcccta caccgtcacc gatgcgagcg gcggcatcgt cgcgcacgac aagcacgtgc
     1681 tgtcgatgaa ggacaacaac cagacggcca acctggacac gctggtggac gcgggcatcc
     1741 gcagcttcaa gatcgagggt cgctacaagg acatgggcta cgtgaagaac atcaccgccc
     1801 actaccgccg ccagatcgac gagatgctgg agcgccggcc cgagctgcgc gcctcgtcgg
     1861 ccggcaaggc catcttcggc ttcacgcccg acccggatcg caacttcaac cgcgagtcca
     1921 ccgactactt cgtcaacggc cgccagatgg acatcggcgc cttcgacacg cccaagcacg
     1981 cgggcctggc gatcggctac gtcaccaagg tggccgccga ccacttcgag gctgagctcg
     2041 acgacccggc cgcgcagctg aacaacggcg acggcctcac gtggctggac ctgcacaagg
     2101 aactcgtggg catgcaggcc aacgtcgtcg cgcggctgcc cggcgcggcc aaccgctggc
     2161 gcgtggtgcc caaccagccg atgtcggagc tgaaggacat ccgatccaag ccccggctgc
     2221 accgcaaccg cgacatgcag tgggatcgcc tgctggaacg caagtcggcc gaccgcctga
     2281 tcggcacgtg gatgacgctg tcggacatcg aaggcggcct gcgcctgtcc gtcaccgacg
     2341 agcgcggctt cgcggcgcac gccgacgcgc tgctggagct gcagccggcg cgcgacgtcg
     2401 agcgcggcat ggcggcgctg cgcgagcacc tgtccaaggt cggcggcacc atcttctcgc
     2461 tgaacgaact gaagatcgcc acgtcgcagc cctggttcgt gcccgcgagc cgcctgaacg
     2521 cgctgcgccg cgaggcgctc gaacgcctgg aacaggtgcg cctggaccag cgcgagatcc
     2581 tgccgcgcgc cctgcccgtc gagccgccgg tgcccttccc cgaggacacg atcagctacc
     2641 tggccaacgt ctacaacgag ccggcgcatg ccttctacgt caagcacggc gtcaaggtgg
     2701 tcgaggcggc ctacgagagc cacgaggagc tcggcgaggt cagcctgatg atcaccaagc
     2761 actgcgtgcg ctggtcgatg tcgctgtgcc ccaagcaggc caagggcgtc accggcgtgc
     2821 agggcaccgt gcgcgccgag ccgctggtgc tgcagaacgg cagcgagaag ctcacgctgc
     2881 gcttcgactg caagccgtgc gagatgcacg tggtcggcaa gatcaagaag caggtgatca
     2941 acgaggcgat ggcggtgccg atgaccttct tccgcacgcg cccggcgccg cagcgcgcgg
     3001 gcaactgagg ctcagccgtc gccctcggcg ccgcgcggcg ccctgccctg gccgccgcgt
     3061 gcgatgcacg cgcgcaggct tgccgggtcg tggacgagat cctgctgcag gtccagcagc
     3121 atcaggtccg gccagatgcc gcgcgccagc tcgaacagct gcgcggcgaa ttcccagcca
     3181 gcgtgcatgc gcaggctgat gacgtcggcg atcgcgctga cgccgacctg gccaccgtcc
     3241 ggcgtgttcc aggtgaaccg tccctcgtca tcgaactcga tcgcgcccgg cgcgaagagc
     3301 gggctcgcgc gcacggccgc gtgcaacggg ccggcgtcgg cgatcggctg gtgcgcgctt
     3361 tcctcgacga gctcggtgag cttgccgggt cgcggcgtcg gcgccaggcg catcagcatg
     3421 aagtcgtagc tcatcgctcg tcctcagccg aaccaccagc ggcgctcggc ccgcaggtcg
     3481 cgctcgaact ggatccagcc gaaggccgcg gccagcaaca gcgcgatcgg cagcagcatc
     3541 agccactggc gcaggcccgt gcgcgcgccc gcctcgtcgg cgcggcggat gccgtcgcag
     3601 ctcttgaaga cgcgactggt gtcgacgacg acctcgtcgc cgtcccgcaa cgcgctccag
     3661 gcgggccagc ccaccttgca cgaatcgacc cggccgccgg cgaagtgcag gcggtagtcg
     3721 tcttcccggt gccagcgcgc gctgcgcgaa tgccgttcca cgcgcagggt ctcgctgcgc
     3781 tcggcggaga acgcgtcgat cgccaggagg atggcgatga tcagcgccgc gaggcgggtg
     3841 aggacgtcca tggcggcgcg cgttcggttg aagggacgac gccagtctag ggacgcgccc
     3901 cgcgccgcgc ttccctcaaa tcggggtgtc gtggcgtcgc ggagacaccg gcggatcgtc
     3961 ggcgccggtc gactgcacgg gcgccggcgg cgacgccgca gtggcgggca gcaccaggac
     4021 atgccccggc gtgatcgccg cggcctgcct ggcggccacg gtcatctcgg cgacggcgcg
     4081 gcgacggctg actggcttgg ggtgttcggg aaagagcatg ggcctccggt gccgggcgac
     4141 aggcccgtgg cgccggagtt ggcatcgccc gccgcgcgtt caagcgcggg aatgcccggg
     4201 gcttgaaatc cacgcgggga cgcgcccgcg cgtcacgcgc tcgcgtgcca cgcgtccacg
     4261 tacgcccgtg cgcgcgaagc gatctccgcc acgctgaact cgggcttgaa caggccgccg
     4321 cccaggccga agccggtggc gccagcgcgg cgccacaggg cgacgttgcc aggcacgatg
     4381 ccgccgaccg gccatagcgg caggcccggc ggcagcaccg tggtcaggcc cgcgaggccc
     4441 gcgtgcgtca tcgcctcggc ggggaagatc ttcagcgcgt cggcgcccgc gcgccaggcc
     4501 gcgaacgcct cggacgcggt gaacacgccc ggcaccgtgc gcatgccgcg ctcgcgcgca
     4561 cggctcacga ccgccgcatc gaaatgcggc gacacgatga gcgtggcgcc ggtgtcgccg
     4621 acggcgtcga cctgcgcggt ctccagcacg gtgcccgcgc cgatgcacgc gtcggccggc
     4681 gcctgcggca cgatcgcggc gatggcggcg accgcgccgg ggcggttcag cggcacctcg
     4741 agcgcgcgga agcccgcgtc gaacagcacg cgcgccacgt cgagcgctcg ctcgggctgc
     4801 aggccgcgca ggatcgccac cagcggcaac gccgggggcc aggtggtgga atcggtcatg
     4861 cggggatgtc ctccgtggcc atggcgcgcc aggcggccag ctggatgcgg cgcgcgtcga
     4921 gcggctcgag gc
//
LOCUS       DATCRO010000215        13713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_474877, whole genome shotgun sequence.
ACCESSION   DATCRO010000215 DATCRO010000000
VERSION     DATCRO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474877"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1677
                     /locus_tag="VIN75_05810"
     CDS             136..1677
                     /locus_tag="VIN75_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY8973713.1"
                     /translation="MTITLTPASTHAISPWIYGLNFATGIANAPPLVTLDRAGGNRWT
                     AYNWENNASNAGSDYLYESDNYLSSSNVPAEAVRALIAADRAAGSASLVTLQMQGWVS
                     ADEAGPVSTSNPPDTTRFKPVVFKKSTQSAAAFTTSPPTSDNAVYMDEFAWALDQKFA
                     GQGIFAANAATPTLVQLDNEPELWNSTHLEIQGSTPISSDAYIARTIALTKALKDQFP
                     GMTIVGPAHYGFGGLYSWQGELGATPSGANWFADKYLAALKTASASYGKPLVDIYDFH
                     WYSEARDPSGNRITSLNTASLTDAQVQAIAQSPRSLWDASYSESSWIHDVLGGPIRLL
                     PRLQAKIAASDPGVGLGITEYNNGGCKHIAGTLAQADNLGIFGAQGVRAATYWPLGSD
                     EPFCLAAFRAFRGFDGGSAVFGDTAVQAASSNVQAVAAYASTDSAHAGRAVFVAINRS
                     TATQQVAFSGLNVTGTAHLWRISASSASGQNPVKPVSAGTQAVSGTSFSVSLPALSVT
                     TIDVF"
     gene            1887..2636
                     /locus_tag="VIN75_05815"
     CDS             1887..2636
                     /locus_tag="VIN75_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973714.1"
                     /translation="MIQHFASMTFQDLFRTMNPTLEIAGFDEHALAGWMRLELKTPDT
                     TLHAFANEEAPPQLLLGVDGDADPALVDRILAALDAVDLRRETASTFALPAAIAPFDT
                     LGFVRGPERRLLRGILEDDGVLQVIPMYACELTAEGRPPPLANFRRWADLLALDRPPQ
                     PWFHLRMSGSPSGLDAPNWSTAGWAGFEDYIDILSRDDQSWLEVRNRKGRILRLPDSH
                     GWDCARERVAAHVGRETRSGRDSEWAQTRAG"
     gene            complement(2760..4019)
                     /locus_tag="VIN75_05820"
     CDS             complement(2760..4019)
                     /locus_tag="VIN75_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HEY8973715.1"
                     /translation="MIHWPAAAFSNLAIWAIAAVATACVIVRPGRWPEAVWAVGGALA
                     LLLTGLLPLSTVMQAVLEGTDVYLFLTGMMLLSELARREGLFDTLAGWAVRHAGGSAR
                     RLFALVYGIGVLVTVFMSNDATAVVLTPAVYAVAKRAGARPLPYLFACALIANAASFV
                     LPISNPANLVVFGEHMPPLVAWLRAFALPSLAAVVATWAVLALLFRGELAEPVDHDSH
                     VPRLSGTGRLAAIGIGGVVLAMLAASALGFPLGAPTLIAAGLAIAAVQASKREAPWGL
                     LQHVSWSVLALVAGLFVLVAGVGRTGLLSALTELLRAAAHAAPRETAWGAGAIVAFAS
                     NLMNNLPAGLIAGTSVNSGMMPDVVRAAVAIGIDLGPNLSVTGSLATILWLVALRREG
                     EHVSAWQFLKVGAVAMPVALVAALACL"
     gene            4195..5007
                     /locus_tag="VIN75_05825"
     CDS             4195..5007
                     /locus_tag="VIN75_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY8973716.1"
                     /translation="MTQDNRPVNLEFDLPAQPQSLVKLAALLREDDINLNAISSLIEG
                     DMSFAAAVMKAVNSPLYGLKGRVQSVQQAVTYLGVREISAIAYETGLQAAFPQVPQLL
                     AVWERASIRGLLMGRLAQALSMEAWSAHTAGLFEECGKAVLYKHAPEKYAPMLASARD
                     DVHLAEMERDVFGCGHDDVGANLCEAWGLTPAAVASVRHHISIHTTGRLPRVSHRYIC
                     VLSALAHTIVNDPGRLDEVAMKLAPQAMLDQTSLLRGLHRVKDKIDDALANA"
     gene            complement(5071..6081)
                     /gene="dusA"
                     /locus_tag="VIN75_05830"
     CDS             complement(5071..6081)
                     /gene="dusA"
                     /locus_tag="VIN75_05830"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY8973717.1"
                     /translation="MPESFDKSTPYSLWRLSIAPMMDWTDRHCRYFHRLITRKTLLYT
                     EMVTTGALLHGNQPRHLDFNDEEHPVALQLGGSEPDALAACAKLAQEWGYDEVNLNCG
                     CPSERVQRGAFGACLMREAELVRDSVAAMRDACTLPVTVKHRIGIDKVESYDFVRDFV
                     GTVAEGGCTVFIVHARNAWLQGLSPKDNRTIPPLRYEFVYRLKKDFPEFTIVLNGGVK
                     GDDAIAAHLEHVDGVMIGREAYHDPWCMADWDARFLGGPARERSREEVEAAMVEYLER
                     LQSAEHHWSQASRHMLGLWNGVTGARRWRQVWSDHKMKTLAPSEVSRLAHAGAPRPAV
                     AA"
     gene            complement(6328..6906)
                     /locus_tag="VIN75_05835"
     CDS             complement(6328..6906)
                     /locus_tag="VIN75_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973718.1"
                     /translation="MNALPGLISLAILVYLVLGPLNELRVDYLRHRLFVIRTRLFEEA
                     AAERISFDSHAYQYTRTVINGMLRFGHRVSFARALTARLVMKKADVRTAVKCQQWAMD
                     KSTDGEREIAQRYLRDANIAVMKHLATSPFVGLMLIPAIAWILMVAAGFSVFTWILRL
                     TPTLQLKLDQAAFEEGRIVRDHRELGTSATAF"
     gene            complement(6906..7322)
                     /locus_tag="VIN75_05840"
     CDS             complement(6906..7322)
                     /locus_tag="VIN75_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973719.1"
                     /translation="MAKKSEDALTRKDLDAFVDRRPFNVAVVEEVGRTVRWLIAFGAA
                     IAFFHYLTLIMSSLAGKTTTANVVLNMLGSLNFSSSLAWAGTVSAVAYGAGQKSLRKR
                     TVNNLHAHIRELETRLDPNRTSSELATTGETNPKDQ"
     gene            complement(7700..8152)
                     /locus_tag="VIN75_05845"
     CDS             complement(7700..8152)
                     /locus_tag="VIN75_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973720.1"
                     /translation="MKRVFLTGLIAATLACATATMPTPARAGDWPMAAGDFWEITGVH
                     VKDGGELAYANFLATEWKADQEYAKSKGWIKGYMILSNSYNRKGEPDLYLVVISERLA
                     SGAESEKRGDEYIAWKKKSNAQLVKESGNRLEVREIESGMLLQELKIK"
     gene            8449..10167
                     /locus_tag="VIN75_05850"
     CDS             8449..10167
                     /locus_tag="VIN75_05850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8973721.1"
                     /translation="MKLLSRLPLRALLTLPYLVLVLGTAIVIGVLSYAAGRDTVDDLS
                     GQLLTETVHRIEQAVEGHVSGSAAVLEVAFPKGIPAPESIAGELPALRERFWLATSVH
                     RDPNNYAYYGDRDGRFIGLWRSSENDAELRLRTEGAGPRSLYHYSGIAGDLGRPDVES
                     RIFEPRERPWYKAGAAVTLQTWTSIYIDFKLQQLVATRARRVNDKAGAFKGVVATDLS
                     LQQVTSFLQRLALSANGVAMVMEKDGNLIGVSRGAHIRKKPDGADVRLNAADSADPLV
                     RATYAAVRGLAEPAGDSRPHTTVFTAPDGSAVQVGYARLTDDAGLDWLIMVAVPRKDF
                     LHGIIDNFRRTVLLAVIASIFVMLTGTLVLSTVTRELRRLAAAARQVGAGSTAPFAAS
                     LRKDELGDLERSFADMQRRLLTDPLTGLANREALVRRVEDRIVAQRRRGDPRPFGVLF
                     VDINHFKKINDEFGHDIGDAVLQELGRRLAAAVDGGDLVARFAGDEFLVLLDAIERRE
                     DAEQVRIKIEQVLRQPLEVLTGSKTLEPALGAAVGVAVYPEDGQDVETLVRHADQQMY
                     LRKRAD"
     gene            complement(10192..11157)
                     /locus_tag="VIN75_05855"
     CDS             complement(10192..11157)
                     /locus_tag="VIN75_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8973722.1"
                     /translation="MSKTALFGAAGAIGQSIAQALAAQGKPYRVVGRSGASLRKSFGA
                     DPLAEVVTWNPDAPESVRAAADGIDTIVYLVGVNYWQFELHPPLMRKTLEGAIAAGVR
                     NFILIGTVYPYGRAGGAPIREDQPRTPHTFKGQMRKAQEDLLLQAHAEGRINATVLRL
                     PDFYGPGVDKSLLDGAARAAVQGGTADMVGPLDVPHEFVFVPDVGPVVARIIDTPAAQ
                     GRVLHLAGAGVTSQQAMVQEMERQTGRPLKRRVAGKTLLRILGLFNPLMREMVEMHYL
                     LTDPVVMDDSLLQSLIGPIRKTPHVEGIRRTLAAVAQPEKALATA"
     gene            11262..12158
                     /locus_tag="VIN75_05860"
     CDS             11262..12158
                     /locus_tag="VIN75_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973723.1"
                     /translation="MADIEPNWEWYRSFMAVLETGSLSAAGRALGMTQPTVGRHVDSL
                     EAALQLKLFTRSFDGFTPTDAALELKPYASNLAATAAALRRVASSQGGGARGTVRLTA
                     SEIVSIEVLPPILANLRDAHPELVVELVPSNKVENLLRRDADIAVRMVAPSQDALVAR
                     RVAAIDVGLHAHRRYLERHGTPKSVEDLARHALIGWDTETAYIRRFQERFPAITRERF
                     AFRTDSDVAQLAAIRAGLGIGLCQARLAGRDPAMVRLFKSSVSLTLETWIAMHADLRD
                     SPRCAVTFAALAEGLAAYARGA"
     gene            complement(12166..>13713)
                     /locus_tag="VIN75_05865"
     CDS             complement(12166..>13713)
                     /locus_tag="VIN75_05865"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEY8973724.1"
                     /translation="ATAGALAAPTRVCVAPVVSSSTAGAFVVGNGTPASCTAAALQSA
                     INAASVITFNCGSAATTIPVYTTLQVPATRSTVIDGGGLVTLDGGGRTRLIEVYSANY
                     RATRTGLTLQHITLANGKAAGTKYVAPNPSNASCSFGWADGAGGAIYVRDAVLHTIDV
                     TFRNNAAATPGPDVGGGAIYALGSVDVSVISSTFDGNSGANGGAVGLLQSDGRFYNSS
                     FTNNQASGTGANYVGGAAQGCAGVAGANQGGAGGNGGAIVIDGGSDVNQTVCGSTFAD
                     NTAGEFGGALFRTADGAARPTTFDRSVFQANRAKTGGALYVQNANPLVITASTLANNV
                     ASTAAGAADLINDNLQVTNTTFAGNVASKGVGGALQLSNASASGFIRNATFSGNQASG
                     GPGYFSAAIFGAMTFPVTNTVFANNLSKDGGSPMQCFFSPGSGTDDMQWPIKRPVGGL
                     NDNSCITGIRFADPLLGALGNNGGYTPTIVPGSSSPLRKAGHNCPATDQRGVARNTAQ
                     CTIGAVE"
BASE COUNT         2101 a         4921 c         4659 g         2032 t
ORIGIN      
        1 acgccgacgc ccgcgccgtc cccgacaccc gcaccgacac cggtccccac gccgccgccc
       61 ccaccggcac cgactcccgc accgcccccg gcgcccacgc cgaccccgcc gcccgcgccg
      121 tcgcccaccc ccgacgtgac catcacgctg acgcccgcct cgacccacgc aatctcgccc
      181 tggatctacg gcctgaactt cgccacgggc atcgccaacg cgccgccgct ggtcacgctc
      241 gatcgcgcgg gcggcaaccg ctggacggcc tacaactggg agaacaacgc ctccaacgcc
      301 ggcagcgact acctgtacga gagcgacaac tacctgagca gcagcaacgt gcccgccgag
      361 gccgtgcgcg cgctgatcgc ggccgaccgt gccgcgggaa gcgcgagcct ggtgaccctg
      421 cagatgcagg gctgggtatc ggccgacgag gccggcccgg tcagcacgtc caatccgccc
      481 gacaccacgc gattcaagcc ggtggtcttc aagaagagca cgcaatcggc cgcggccttc
      541 acgacgtcgc cgccgacatc cgacaacgcc gtctacatgg acgagttcgc gtgggcgctc
      601 gaccagaagt tcgctggcca gggcatcttc gccgcgaacg ccgcgacgcc cacgctcgtg
      661 cagctcgaca acgagcccga gctgtggaac tccacgcacc tggagatcca gggcagcacg
      721 ccgatctcgt cggacgcgta catcgccagg accatcgcgc tcaccaaggc gctgaaggac
      781 cagttccccg gcatgaccat cgtcgggccc gcacactacg gcttcggcgg cctgtactca
      841 tggcagggcg agctcggggc gacgccctcg ggcgccaact ggttcgccga caagtacctg
      901 gccgcgctga agacggccag cgcgagctac ggcaagccac tggtcgacat ctacgacttc
      961 cactggtact ccgaagccag ggatccgtcc ggcaaccgca tcaccagcct gaacaccgcg
     1021 tcgctgaccg acgcacaggt gcaggccatc gcgcagagcc cgcgcagcct gtgggacgcg
     1081 agctactcgg agtcgtcctg gatccacgac gtgctgggcg gcccgattcg gctgctgccg
     1141 cgcctgcagg ccaagatcgc cgcgtccgat ccgggcgtcg ggctgggcat caccgagtac
     1201 aacaacggcg gctgcaagca catcgcgggc acgctcgcgc aggccgacaa cctcggcatc
     1261 ttcggcgcgc agggcgtgcg cgccgccacg tactggccgc tcggcagcga cgagcccttc
     1321 tgcctggccg cgttccgcgc cttccgcggc ttcgacggcg gcagcgcggt cttcggggac
     1381 accgcggtgc aggccgcgtc gagcaacgtg caggccgtcg cggcctacgc cagcaccgac
     1441 agcgcgcacg ccggccgcgc cgtgttcgtc gcgatcaacc ggtcgacggc cacgcagcag
     1501 gtcgcgttct cggggctgaa cgtcaccggc accgcgcacc tgtggcgcat cagcgcgtcg
     1561 tccgcgtcgg gccagaaccc cgtcaagccc gtgtccgcgg gcacgcaggc ggtgtcgggc
     1621 acgtcgttca gcgtgtcgct gccggcgttg agcgtgacga cgatcgacgt cttctagccc
     1681 tccccagggc gcatcctggg gatgtgcggc aggcggggcc atgcgcatcc tgcgccgcga
     1741 gggcctgggc ggcgcgcgtg acgcgaggcg agggctgcct gtgtgccggc gccggcgcgt
     1801 ccgatcgcct cacccgcgcg ccccgagcgc gcgaaacccc gccgtttgcc gcgctgccgc
     1861 gctggccgcg ccggcctcgc catgcaatga tccagcactt cgcgtcgatg accttccagg
     1921 accttttccg cacgatgaat cccacgctcg agatcgccgg cttcgacgag cacgcgcttg
     1981 cgggctggat gcgtctcgag ttgaagacgc cggacaccac gctgcacgcc ttcgccaacg
     2041 aggaggcgcc gccgcaattg ctgctgggcg tggacggcga tgccgacccg gcgctggtcg
     2101 accgcatcct ggccgcgctc gacgccgtcg acctgcggcg cgagacggcg tccaccttcg
     2161 cgttgccggc cgccatcgcg ccgttcgaca cgctgggctt cgtgcgcggc cccgagcgcc
     2221 gcctgctgcg cggcatcctc gaggacgacg gcgtcctgca ggtcatcccg atgtacgcct
     2281 gcgagctgac ggccgaaggc cgcccgccgc cgctggccaa ctttcgccgc tgggccgacc
     2341 tcctcgcgct cgaccgaccg ccccagccat ggttccattt gcgcatgagc ggctcaccgt
     2401 ccgggctcga cgcgccgaac tggagcacgg cgggctgggc gggcttcgag gactacatcg
     2461 acatcctctc gcgcgacgac cagtcctggc tggaagtgcg caaccgcaag ggacgcatcc
     2521 tgcgcctgcc cgactcgcac ggttgggact gcgcccgtga acgcgtcgcc gcccacgtcg
     2581 gacgcgagac gcgcagcggc cgcgacagcg aatgggcgca gacacgggcg ggctgagccc
     2641 gcgccgcacc gaagaaggcc cgtgagtccg gtcacgcatt tcctgctgtc ctggacgaag
     2701 cgccgcgcct gatgcagcct tcggcccggg acgcccgcgg catcgccgac agctgcgcgt
     2761 cacaggcacg ccagcgccgc caccaacgcc accggcatcg ccaccgcccc caccttcagg
     2821 aactgccagg ccgacacgtg ctcgccctcg cggcgcagcg ccaccagcca caggatggtg
     2881 gccagcgaac cggtcaccga caggttcggc ccgaggtcga tgccgatggc cacggccgcg
     2941 cgcacgacgt cgggcatcat cccggagttg acggacgtgc cggcgatcag gccggcgggc
     3001 aggttgttca tcaggttgga cgcgaaggcg acgatggcgc ccgcgcccca cgcggtctcg
     3061 cgcggcgccg cgtgcgcggc cgcgcgcagc agctcggtca gcgcggacag cagccccgtc
     3121 cgtccgacgc cggccaccag cacgaacagg ccggcgacca gcgcgagcac gctccacgag
     3181 acgtgctgca gcagccccca gggcgcctcg cgcttgctcg cctgcacggc ggcgatggcc
     3241 aggcccgcgg cgatcagcgt gggggcgccc agcgggaagc cgagcgccga ggccgcgagc
     3301 atcgccagca ccaccccgcc gatgccgatg gccgccagcc ggcccgtgcc cgacaggcgc
     3361 ggcacgtgcg agtcgtggtc gaccggctcg gccagctccc cgcggaacag cagcgccagc
     3421 acggcccagg tcgcgacgac ggcggccagc gatggcagcg cgaaggcgcg cagccaggcc
     3481 accagcggcg gcatgtgctc gccgaacacg acgaggttgg ccgggttgga gatcggcagc
     3541 acgaagctgg cggcgttggc gatcagcgcg caggcgaaca ggtagggcag cggccgcgcg
     3601 ccggcccgct tggcgacggc gtagacggcc ggggtgagca cgaccgcggt cgcgtcgttg
     3661 gacatgaaca ccgtcaccag caccccgatg ccgtagacca gcgcgaacag ccgccgcgcc
     3721 gaaccgccgg cgtggcgcac cgcccagccg gccagggtgt cgaacagccc ctcgcggcgc
     3781 gccagctccg acagcagcat catgccggtc aggaacaggt agacgtcggt gccctcgagc
     3841 acggcctgca tcaccgtcga caggggcagc aggcccgtca gcagcagcgc cagcgcgccg
     3901 cccacggccc agacggcttc gggccagcgg cccggccgca cgatgacgca ggcggtcgcc
     3961 acggcggcga tggcccagat ggccaggttg gagaaagcgg cggcgggcca gtggatcatg
     4021 tgttcgcagg gggtgggacg aggtggcggg cgccccttct cggcaggcgc cgtgccctgc
     4081 attgtcatcg cgacaaacgg atgccttttg gcacccattt cgcccgcgcc ggacgccctg
     4141 gaaggactag catccaggct gaccgtccct cagtgtgacc gccgcggagt gcccatgacg
     4201 caagacaacc gtcccgtgaa cctcgagttc gacctgcctg cccagccgca gtcgctggtc
     4261 aagctcgctg cgctgctgcg cgaggacgac atcaacctga acgccatcag cagcctgatc
     4321 gagggcgaca tgtcgttcgc ggccgccgtg atgaaggccg tcaactcgcc gctgtacggg
     4381 ctcaagggtc gcgtgcagag cgtgcagcag gccgtcacct acctgggcgt gcgcgagatc
     4441 tcggccatcg cctacgagac cggcctgcag gccgccttcc cgcaggtgcc gcagctgctg
     4501 gccgtgtggg agcgcgcgag catccgcggc ctgctgatgg gccggctcgc ccaggcgctg
     4561 tcgatggagg cctggagcgc gcacaccgcc ggcctgttcg aggaatgcgg caaggccgtg
     4621 ctgtacaagc acgcgcccga gaaatacgcc ccgatgctcg cctcggcccg cgacgacgtg
     4681 cacctggccg agatggagcg cgacgtcttc ggctgcggcc acgacgatgt cggcgccaac
     4741 ctgtgcgagg cctggggcct gacgccggcg gccgtcgcca gcgtgcgcca ccacatcagc
     4801 atccacacga cgggccgcct gccgcgggtg tcgcaccgct acatctgcgt gctgtccgcc
     4861 ctcgcccaca ccatcgtcaa cgaccccggg cgcctcgacg aggtcgccat gaagctggcg
     4921 ccgcaggcga tgctggacca gacctcgctg ctgcgcggcc tgcaccgcgt caaggacaag
     4981 atcgacgacg cgctggccaa cgcctgagcg ccaccgcgcc gacgcaaggg gcagccgagg
     5041 ctgccctttt tcgctcctgc gcggcccgcc tcaggccgcg accgccggcc tcggcgcacc
     5101 ggcgtgcgcg agccgcgaca cctccgacgg cgccagcgtc ttcatcttgt ggtccgacca
     5161 cacctggcgc cagcgccgcg cgcccgtgac gccgttccac aatcccagca tgtggcgcga
     5221 ggcctgcgac cagtggtgct cggcggactg cagccgctcc aggtactcga ccatcgccgc
     5281 ctcgacctcc tcgcgcgagc gctcgcgcgc ggggccgccc aggaagcgcg cgtcccagtc
     5341 ggccatgcac catgggtcgt ggtaggcctc gcggccgatc atcacgccat cgacgtgctc
     5401 caggtgcgcc gcgatcgcgt cgtcgccctt cacgccgccg ttcagcacga tggtgaactc
     5461 cgggaaatcc ttcttgagcc ggtagacgaa ctcgtagcgc agcggcggga tcgtgcggtt
     5521 gtccttcggg ctcaggccct gcagccaggc gttgcgcgcg tgcacgatga acaccgtgca
     5581 gccgccttcg gccacggtgc cgacgaagtc gcgcacgaag tcgtagctct cgaccttgtc
     5641 gatgccgatg cggtgcttga ccgtcaccgg cagcgtgcag gcatcccgca tcgccgcgac
     5701 gctgtcgcgc accagctcgg cctcgcgcat caggcacgcg ccgaacgcgc cgcgctgcac
     5761 ccgctcgctg gggcagccgc aattgaggtt gacctcgtcg tagccccatt cctgcgccag
     5821 cttcgcgcag gccgccagcg cgtcgggctc gctgccgccc aactgcagcg cgaccgggtg
     5881 ctcctcgtcg ttgaagtcga ggtgccgcgg ctggttgcca tgcagcagcg cgccggtggt
     5941 caccatctcg gtgtacagca gcgtcttgcg cgtgatcagg cggtggaagt agcgacagtg
     6001 acggtcggtc caatccatca tcggtgcaat ggagagccgc cacagactgt aaggcgttga
     6061 tttatcaaaa gattccggca tttcgggtgc tcacgtgcat cacgaaaatg cacaaacttt
     6121 ccctgggata cactccgcgc tcgctatcga tttgctacct gcgcaggggc atcgatcgcg
     6181 cgaagtgcta ccagggaggt tcaggggaag gccagcatta tcccagtcaa ggggaagtgg
     6241 cgagcgcagg tgcgccatcc cattgaagcc gtcagcgcca ataaaaaagg gcccgctcat
     6301 ccgggccctt cgcgcttgac gacgcactca aaacgcagtt gcagacgttc cgagctctcg
     6361 gtgatcccgc acgatgcgac cttcctcgaa cgctgcctga tccagcttca attgcagcgt
     6421 cggcgtcagg cgcaagatcc acgtgaagac gctgaatcct gccgcgacca tgaggatcca
     6481 cgcgatcgcc ggaatcagca tcagaccgac gaatggcgaa gtcgcaaggt gcttcatgac
     6541 ggcgatgttg gcgtcgcgca gatatcgctg agcaatctcg cgctctccat cagtggactt
     6601 gtccattgcc cattgctggc acttgaccgc cgtccgaacg tcagcctttt tcatcaccaa
     6661 gcgagcagtc agcgcccggg caaaactaac ccgatgtcca aagcgcaaca tgccattgat
     6721 gacggtgcgc gtgtattggt acgcgtggct gtcaaacgag atacgctcag ctgcagcttc
     6781 ctcgaacagc ctggtacgaa tcacaaacag acgatggcgc aggtaatcca cgcgcagctc
     6841 gttgagcggt ccaagcacca agtacacaag gatcgcgagc gagatgaggc cgggcagtgc
     6901 gttcatcatt ggtcctttgg gttggtttcg ccggtcgtag cgagttcgct tgaggtacgg
     6961 tttggatcca gtcgagtctc cagctcgcga atatgcgcgt gcagattgtt gaccgtgcgc
     7021 tttcgcaggc tcttctgacc tgctccatac gcgacagcac ttaccgtgcc cgcccaggcg
     7081 agcgacgacg aaaagttcag gctaccgagc atgttcagca cgacattggc ggtcgtcgtc
     7141 tttcccgcca gggagctcat gatcagcgtc aagtagtgaa agaaggcaat cgccgcgccg
     7201 aacgcgatca accatcttac ggttcgaccc acttcttcga cgaccgccac gttgaagggg
     7261 cgacgatcga cgaaggcgtc aagatccttg cgcgtcagcg catcctcgct cttttttgcc
     7321 aagttgcctc ctctgatatg aggggatatt actcaacaac aagagtttca ggtaacggca
     7381 tcgacccgtc accacggcct tcagaaggcc cgagcgcagg cgtctgcacg cagtggtcag
     7441 tgctaccggt tcgctaccag cgccccgcaa acccgcatga attcgcaccc gtcgtccaat
     7501 ccatcatcgg ggcgacggac aggcgccagg ggctaacttg ttgattttgc tcggaaatca
     7561 tgcaccgcca ttgtcgccgc gatcggcctg cggcgcaaag tgcgggcttc tggcgatcga
     7621 tggctggcgc ggccggcctg actttcgacg cgggcttgcc ctgcgcaggc ccggcgccga
     7681 agcgcgcggg ccggcgccgt cacttgatct tgagctcctg caggagcatg ccgctctcga
     7741 tctcgcgcac ctccagccgg ttgcccgact ccttgaccag ctgcgcgttg ctcttcttct
     7801 tccaggcgat gtattcgtcg ccccgcttct ccgactcggc gccgcttgcc aggcgttcac
     7861 tgatgaccac caggtacagg tcgggctcgc ccttgcggtt gtaggagttg ctcaggatca
     7921 tgtagccctt gatccagccc ttggacttcg cgtactcctg gtccgccttc cattcggtcg
     7981 ccaggaagtt cgcatacgcg agctcgccgc cgtccttcac gtggaccccc gtgatctccc
     8041 agaagtcgcc ggccgccatc ggccagtcgc ccgcccgcgc gggggtgggc atcgtcgccg
     8101 tcgcgcatgc gagcgtggcc gcgatcaagc ccgtcaagaa aacacgcttc atcgtcttcc
     8161 ccttcgcgac caccatggcc gcgccgggca agctagcact gatgagcagc gcgatcaacg
     8221 gcctgcgccc ctcgagactc gggggatgcc gcgtccgcgc aggcttggcg gatgcggccg
     8281 ggcgtgactt gcgcctgcct ttcggtgccc gtcgcgccgg cacgcccaac gctgcgggcg
     8341 gatccagcgt ccggtccagg ccacaatcgg ccccaagcgc gcgacgggcg ggccctggag
     8401 ggggacgccg caccgtcgcc gcgacccggc gatggattca agaactagat gaagctcctc
     8461 tcgcgcctgc ccctgcgggc cctgctcacg ctgccctacc tcgtgctcgt gctcggcacg
     8521 gccatcgtca tcggcgtgct gtcgtatgcc gccggccgcg acacggtcga cgacctgtcc
     8581 gggcagctcc tcacagagac cgtgcaccgc atcgagcagg cggtcgaggg gcacgtgtcg
     8641 ggctcggccg cggtgctgga ggtggcgttc cccaagggca tcccggcccc cgagtccatc
     8701 gcgggcgagc tgccggccct gcgcgaacgc ttctggctgg cgacgtcggt gcaccgcgac
     8761 ccgaacaact acgcctatta cggcgaccgc gacggccgct tcatcggact gtggcgctcg
     8821 tcggagaacg acgccgagct gcgcctgcgc acggaaggcg ccggcccgcg cagcctctac
     8881 cactacagcg ggatcgcggg cgacctgggc cgcccggacg tcgagtcgcg catcttcgag
     8941 ccgcgcgagc ggccctggta caaggccggc gccgcggtca cgctgcagac ctggacctcg
     9001 atctacatcg acttcaagct gcagcagctg gtggccacgc gcgcgcggcg cgtcaacgac
     9061 aaggccggcg ccttcaaggg cgtggtcgcc accgacctgt cgctgcagca ggtcacctcc
     9121 ttcctgcaac gcctggccct cagcgccaac ggcgtggcca tggtgatgga gaaggacggc
     9181 aacctgatcg gcgtctcgcg cggcgcgcac atccgcaaga agcccgacgg cgccgacgtg
     9241 cggctgaacg cggccgacag cgccgatccg ctggtgcgcg cgacctacgc cgcggtgcgc
     9301 ggcctggccg aacccgccgg cgactcgcgc ccgcacacca cggtcttcac cgcgcccgac
     9361 ggctccgccg tgcaggtggg ctacgcgcgc ctgaccgacg atgccgggct cgactggctg
     9421 atcatggtgg ccgtcccgcg caaggacttc ctgcacggca tcatcgacaa cttccgccgc
     9481 accgtgctgt tggccgtgat cgcgtcgatc ttcgtcatgc tgaccggcac gctggtgttg
     9541 tcgaccgtca cgcgcgagct gcgccgcctg gcggccgccg cgcgccaggt gggcgcaggc
     9601 agcaccgcgc cgttcgcggc atcgcttcgc aaggacgaac tcggcgacct ggagcgcagc
     9661 ttcgccgaca tgcagcgccg cctgctgacc gacccgctca ccggcctggc caaccgcgag
     9721 gcgctggtcc ggcgtgtcga ggaccgcatc gtcgcccagc gccgccgcgg cgacccgcgg
     9781 ccgttcggcg tgctgttcgt cgacatcaac cacttcaaga agatcaacga cgagttcggc
     9841 cacgacatcg gcgacgcggt gctgcaggag ctgggccgcc ggctcgctgc ggccgtcgac
     9901 ggcggcgacc tcgtggcgcg cttcgccggc gacgagttcc tcgtgctgct ggacgcgatc
     9961 gaacgccgcg aggacgccga gcaggtgcgc atcaagatcg agcaggtgct gcgccagccg
    10021 ctggaggtgc tgaccggcag caagacgctc gaaccggcgc tgggcgcggc cgtcggcgtg
    10081 gccgtctacc ccgaggacgg gcaggacgtg gagaccctgg tgcgccacgc cgaccagcag
    10141 atgtacctgc gcaagcgggc cgactgagcg gccggctcgc aaggacgccg gtcaagccgt
    10201 cgcgagcgcc ttctccggct gcgccaccgc ggccagcgtg cggcggatgc cctcgacatg
    10261 cggcgtcttg cggatcgggc cgatcagcga ctgcagcagg ctgtcgtcca tcaccaccgg
    10321 gtcggtcagc aggtagtgca tctccaccat ctcgcgcatg agcgggttga acagccccag
    10381 gatgcgcagc agcgtcttgc cggcgacgcg gcgcttgagc ggacgacccg tctggcgctc
    10441 catctcctgc accatcgcct gctgcgaggt cacgcccgcg cccgcgaggt gcagcacgcg
    10501 gccctgcgcc gccggggtgt cgatgattcg agcgacgacg gggccgacgt ccggcacgaa
    10561 gacgaattca tgcggcacgt cgagcgggcc caccatgtcg gccgtgccgc cctggaccgc
    10621 ggccctggcg gcgccgtcca gcaggctctt gtcgacgccg gggccgtaga agtcgggcag
    10681 gcgcagcacc gtggcgttga tgcggccctc ggcatgggcc tgcagcagca ggtcttcctg
    10741 cgccttgcgc atctgcccct tgaaggtgtg cggcgtgcgc ggctggtcct cgcggatcgg
    10801 cgcgccgccg gcgcggccat acgggtacac ggtgccgatc aggatgaagt tgcgcacgcc
    10861 cgcggcgatc gcgccctcga gggtcttgcg catcagcggc gggtgcagtt cgaactgcca
    10921 gtagttcacg ccgaccaggt agacgatggt gtcgatgccg tcggcggcgg cacgcaccga
    10981 ctcgggcgcg tcggggttcc aggtgacgac ctcggcgagc gggtcggcgc cgaagctctt
    11041 gcgcaggctg gccccggagc ggccgacgac gcgatagggc ttgccttgcg cggcgagcgc
    11101 ctgggcgatg gattggccga tggcgccggc ggcaccgaac agggcggtct tggacatgac
    11161 ttttctcctt gggggtttgc tgcgatggac gcagtttgcg gaaagcctgg gcatttgaaa
    11221 attgcctaga atcgcattgg tgctatacga tttcgcatac cgtggccgac atcgaaccga
    11281 actgggagtg gtaccgcagt ttcatggcgg tgctggagac gggctcgctg tcggcagcgg
    11341 gccgcgcgct gggcatgacg cagcccaccg tgggccgcca tgtcgacagc ctcgaggccg
    11401 cgctgcagct caagctgttc acgcgctcgt tcgacggctt cacgccgacc gacgcggcgc
    11461 tcgagctcaa gccctacgcg agcaacctcg cggccacggc cgcggcgctg cgccgcgtcg
    11521 cgtccagcca gggcggcggc gcgcgcggga cggtgcggtt gacggccagc gagatcgtca
    11581 gcatcgaggt gttgccgccc atcctcgcga accttcgtga cgcacacccg gagctcgtcg
    11641 tcgagctggt gccgtccaac aaggtggaga acctgctgcg ccgcgacgcc gacatcgcgg
    11701 tgcggatggt ggcgccgtca caggacgcgc tcgtcgccag acgcgtggcg gcgatcgacg
    11761 tgggcctgca tgcgcaccgc cgctacctcg agcggcacgg cacgcccaag tcggtcgagg
    11821 acctggcgcg ccatgcgttg atcggctggg acaccgagac ggcctacatc cgccgcttcc
    11881 aggaacgctt tccggcgata acgcgcgagc gcttcgcgtt ccgcacggac agcgacgtgg
    11941 cgcagctggc cgccatccgc gcggggctgg gcatcgggct gtgccaggca cggctcgcgg
    12001 gacgcgatcc ggccatggtg cggctgttca agtcgtcggt ctcgctgacc ctggagacct
    12061 ggatcgcgat gcacgccgac ctgcgcgaca gcccgcgctg cgccgtcacg ttcgccgcgc
    12121 tggccgaggg cctcgcggcc tacgcgcgcg gcgcgtgagg cgccctcact ccacggcgcc
    12181 gatcgtgcac tgcgccgtgt tgcgcgcgac gccgcgctgg tcggtcgccg ggcagttgtg
    12241 gccggccttg cgcagcgggc tgctcgagcc gggcacgatc gtgggcgtgt agccgccgtt
    12301 gttgccgagc gcgcccagca gcggatccgc gaagcggatg ccggtgatgc agctgttgtc
    12361 gttcaggccg ccgaccgggc gcttgatggg ccactgcatg tcgtcggtgc ccgagcccgg
    12421 cgagaagaag cactgcatcg gcgagccgcc gtccttgctg aggttgttgg cgaacacggt
    12481 gttggtgacc gggaacgtca tcgcgccgaa gatcgccgcg ctgaagtagc ccgggccgcc
    12541 gctggcctgg ttgccgctga acgtcgcgtt gcggatgaag ccgctcgcgc tcgcgttgga
    12601 cagttgcagg gccccgccga cgcccttgct ggcgacgttg cccgcgaagg tcgtgttggt
    12661 gacctgcagg ttgtcgttga tcaggtcggc ggcgccggcg gcggtggagg cgacgttgtt
    12721 ggcgagcgtc gaggcggtga tgaccagcgg gttcgcgttc tgcacgtaca gcgcgccgcc
    12781 ggtcttggcg cggttggcct ggaacaccga gcggtcgaag gtggtcggcc gcgccgcgcc
    12841 gtcggccgtg cggaacagcg cgccgccgaa ctcgccggcc gtgttgtcgg cgaaggtgct
    12901 gccgcagacg gtctggttca cgtccgaacc gccgtcgatg acgatcgcgc cgccgttgcc
    12961 gcccgcgccg ccctggttcg cgccggccac gcccgcgcag ccctgggccg cgccgccgac
    13021 gtagttcgcg ccggtgccgc tggcctggtt gttggtgaac gacgagttgt agaagcggcc
    13081 gtcgctctgc agcagcccga ccgcgccgcc gttggcgccg ctgttgccgt cgaaggtcga
    13141 gctgatgacg gacacgtcca ccgagccgag cgcgtagatc gcgccgccgc cgacgtcggg
    13201 gcccggcgtg gcagcggcat tgttgcggaa ggtcacgtcg atcgtgtgca gcacggcgtc
    13261 gcgcacgtag atcgcgccgc ccgcgccgtc ggcccagccg aacgagcagc tcgcgttgct
    13321 cgggttcggg gcgacgtact tcgtgccggc ggccttgccg ttggccagcg tgatgtgctg
    13381 gagcgtcagg cccgtcctcg tggcgcggta gttcgcgctg tagacctcga tcagccgcgt
    13441 gcggccaccg ccgtcgagcg tcacgaggcc accgccgtcg atgacggtcg agcgcgtggc
    13501 gggcacctgc aaggtcgtgt agaccgggat cgtggtcgcg gcactgccgc agttgaacgt
    13561 gatgacagac gcggcgttga tggccgactg cagcgcggcc gcggtgcagc tggccggcgt
    13621 gccgttgccg acgacgaacg cgcccgcggt gctggacgag accacgggcg cgacgcacac
    13681 gcgcgtgggc gcggcgagcg cgccggccgt ggc
//
LOCUS       DATCRO010000216         7613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_677334, whole genome shotgun sequence.
ACCESSION   DATCRO010000216 DATCRO010000000
VERSION     DATCRO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677334"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..520
                     /locus_tag="VIN75_05870"
     CDS             101..520
                     /locus_tag="VIN75_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973725.1"
                     /translation="MAFTNVWEPGGVATRFTGFVTAEEYVRSAEDICADPRFDGLQFV
                     IKDLLAIDDHSIEPEACDPIAAIRYGARYSNPNIYLVLLTTDERLVPFAHPRADSFLH
                     GLYETHAFPSDAAARRWLAAQPPLGRSTGRLRRTSSA"
     gene            complement(586..1551)
                     /locus_tag="VIN75_05875"
     CDS             complement(586..1551)
                     /locus_tag="VIN75_05875"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY8973726.1"
                     /translation="MNRRALPAALSNLRLPVIGSPLFIISNPKLVIEQCKAGIVGSMP
                     SLNARPAELLDEWLAEITETLAAHDRAHPEAPSAPFAINQIVHKSNDRLEHDMQVCAK
                     YKVPIIITSLGAREDVNAAVHGWGGIVLHDIINNTFAHKAIDKGADGLIAVATGAGGH
                     AGVKNPIALIHEIREWFDGPLALSGAIATGDGVLAAQAMGADFGYIGSAFIATDEARA
                     SEDYKKMIVASTSDDIVYSNLFTGVLGNYLRGSITQAGLDPDNLPTSDASKMNFAGAG
                     AAKAWKDIWGAGQGVGAVKGIVPAATLVKRLSDEYAAARRRLALA"
     gene            complement(1554..3338)
                     /locus_tag="VIN75_05880"
     CDS             complement(1554..3338)
                     /locus_tag="VIN75_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8973727.1"
                     /translation="MTYRAPVKDMLFVMKELAGIDELARMPAFEDAGFDTAQAVLEEC
                     ARFNEDVVAPLNAEGDRNPSFFKDGQVIATPGFKEAFKQFGEGGWQGLQHPTDFGGQG
                     LPKLIGAACGEMINSANVSFALCPLLTDGAIEALLTAGSDEQKARYLPNMLSGKWTGS
                     MNLTEPQAGSDLALVRTRAEPQADGTYKVFGTKIFITWGEHDMAENIVHLVLARVAGA
                     PEGVKGISLFIVPKFLVNADGSLGARNDAHCVSIEHKMGIKASPTCVLQFGDHGGAVG
                     YLVGQENRGLEYMFIMMNAARFGVGVQGIAVAERAYQKAVEYARERVQSRPVDGSMTT
                     AAPIIHHPDVRRMLMTMRAYTEACRAMALVAASAFDAAHEHPDASVRAENQAFYEFMV
                     PLVKAYSTEMSIEVASLGVQVHGGMGFIEETGAAQYMRDSKILTIYEGTTAIQANDFV
                     GRKTARDGGTAARAIAARIESTEKALAASSNKDAQAFGKRLKAAREAFVDAVAFIASN
                     AKGSPNAAFAGSVPYLMLAGNLVGGWQMGRALLVAAERTGDDAAFMAAKIATARFYGD
                     HILSKAQGIRDSIVDGAEGTLAMPLESF"
     gene            complement(3544..4476)
                     /locus_tag="VIN75_05885"
     CDS             complement(3544..4476)
                     /locus_tag="VIN75_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8973728.1"
                     /translation="MTTLVIAEHDNASLKGATLNTITAAAQVGGDVHVLVAGHNAGAV
                     ATAAAAVAGVAKVLHADSAALAEALAENLAAQVLAVASGYSHIFFPSTANGKNTAPRV
                     AAKLDVGQLSDISKVVSADTFERPIYAGNAIATVQCSQPVKVATVRTTGFDPAAATGG
                     SAAVETIAAVADGGKSTFVGKEIAKLDRPELTAAKIIVSGGRAMGSSEKFNEVLTPLA
                     DKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVACGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFSVADYGLVADLFVAVPEMIKAL"
     gene            complement(4500..5249)
                     /locus_tag="VIN75_05890"
     CDS             complement(4500..5249)
                     /locus_tag="VIN75_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8973729.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVVTEIIAVSCGVTQCQETLRTAMAIGADRGILVETDAELQPLAVAKLLK
                     ALVDKEQPGLVILGKQAIDDDCNQTGQMLAALADLPQATFASKVELTADSVTVAREVD
                     GGSETLRLSLPAIVTTDLRLNEPRYVTLPNIMKAKKKPLEIVKPDALGVDVAPRIKTL
                     KVSEPPKRGAGVKVPDVATLVAKLKNEAKVI"
     gene            5420..6262
                     /gene="murI"
                     /locus_tag="VIN75_05895"
     CDS             5420..6262
                     /gene="murI"
                     /locus_tag="VIN75_05895"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8973730.1"
                     /translation="MSRQANPAPASPSAAPQPVRIGIFDSGVGGLSVMQAVRARLPHA
                     ELLYAADTAFAPYGDRTEEFLCDRSERIARFLRDQGAQTIVVACNTATAAAVARLRAT
                     WPALPVVGVEPGVKPAVALSATHRVGVLATTRTLASEKFRRLAEAHAGDAQLVLQPCP
                     GLADAIESADDAAIDVLVQRYCAPLRDAGADVAVLGCTHYVFARERFERALPGVQLVD
                     TAEAIARQTARFADELVARGEADADAARPSVLHAWSSGSAHVLASFVQRWLGLRVDVR
                     EMRC"
     gene            6271..7092
                     /locus_tag="VIN75_05900"
     CDS             6271..7092
                     /locus_tag="VIN75_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973731.1"
                     /translation="MSTPAPAAARVTRERPSTQMRRLRHAPLGRCMYCLAPGGPGIVL
                     TEEHLVPRALGGRLTLRDAVCEPCRRLTGRHEQLTLDREFAVPRTLLAMKRRRARAKG
                     PGRLPAVVLEGGGSPVTLPADAFPRTFALPAFERAGLLAGIDRADVPAHVDFVACTLD
                     LGSIARRTAAAPPALPDPAAYAHSIAKWAYALAVAERGPACCDTEPLRDLVLGRRDDV
                     FEFVGSASERAPASREWLHDVAFHEQDGWLVATLSLFASAGMAPYEVVIGRLAGR"
     gene            7173..>7613
                     /locus_tag="VIN75_05905"
     CDS             7173..>7613
                     /locus_tag="VIN75_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8973732.1"
                     /translation="MRIRLLAAATLSAALLAPPTPSRAADAPLTGDTAAAAQVWSTFE
                     HWLKAYEAGDLDGIMAIFDRDVIFEFQGGPDATADMLRRDYADDLKARAAGTRWVPRV
                     EEVHADGNTAFVRSMWELHVGDKVTQRNRSMDVFQRRDGHWTILR"
BASE COUNT         1029 a         2730 c         2633 g         1221 t
ORIGIN      
        1 gccctgaacc ggcccttgcc gggggtcggc gtcaggttca atattcagct ccgggacagg
       61 cgccatgtcg gcgccttgcc cgcctggatg tggacgcgcg atggctttca ccaacgtctg
      121 ggaacccgga ggggtcgcga cccgtttcac cggcttcgtc acggccgagg agtacgtgcg
      181 ctcggcggag gacatctgcg ccgacccgcg cttcgacggc ctgcagttcg tcatcaagga
      241 tctgctggcc atcgacgacc actcgatcga gcccgaggcc tgcgacccga tcgccgcgat
      301 ccgctacggc gcgcgctact cgaacccgaa catctacctc gtgctgctga cgaccgacga
      361 gcgcctggtg ccgttcgccc atccgagggc ggactcgttc ctgcacggcc tgtacgagac
      421 gcacgcgttc ccgagcgacg ccgcggcgcg ccgctggctg gccgcccagc ccccgctggg
      481 gcgctcgacc gggcgcctgc gcaggacgtc ctccgcatga agaaggcgcc ccgcggggcg
      541 cctttcgatt gccacgaggg tcgcgcgcga cgcgcgcgcc tgcgctcagg cgagcgcgag
      601 gcgccggcgc gcggccgcgt actcgtcgga caggcgcttc acgagcgtcg ccgccggcac
      661 gatgcccttg accgcgccca cgccctggcc cgcgccccag atgtccttcc aggccttggc
      721 ggcgcccgcg ccggcaaagt tcatcttgct cgcgtcgctg gtgggcaggt tgtcggggtc
      781 gaggccggcc tgcgtgatcg agccgcgcag gtagttgccc agcacgcccg tgaacaggtt
      841 ggagtagacg atgtcgtcgc tggtggaggc gacgatcatc ttcttgtagt cctcggacgc
      901 gcgcgcctcg tcggtggcga tgaaggccga gccgatgtag ccgaagtccg cgcccatcgc
      961 ctgcgcggcc agcacgccgt cgccggtggc gatcgcgccg gacaacgcca gcgggccgtc
     1021 gaaccactcg cggatctcgt ggatcagcgc gatcgggttc ttgacgccgg catggccgcc
     1081 cgcgcccgtg gccacggcga tcaggccgtc ggcgcccttg tcgatcgcct tgtgcgcgaa
     1141 cgtgttgttg atgatgtcgt gcaggacgat gccgccccag ccatgcaccg cggcgttgac
     1201 gtcctcgcgc gcgcccagcg acgtgatgat gatgggcacc ttgtacttgg cgcacacctg
     1261 catgtcgtgc tcgaggcggt cgttgctctt gtgcacgatc tggttgatgg cgaacggcgc
     1321 cgacggcgcc tcgggatggg cgcggtcgtg ggcggccagc gtctcggtga tctcggcgag
     1381 ccactcgtcg agcagttcgg ccggacgcgc gttgagcgac ggcatcgagc cgacgatgcc
     1441 ggccttgcac tgctcgatca ccagcttggg gttgctgatg atgaacagcg gcgagccgat
     1501 gacgggcagg cgaaggttcg acagcgcggc gggaagggca cgacgattca tgttcagaac
     1561 gactccaggg gcatggcgag ggtgccttcg gcgccgtcga cgatcgagtc gcggatgcct
     1621 tgggccttgc tgaggatgtg gtcgccgtag aagcgcgcgg tggcgatctt ggcggccatg
     1681 aacgcggcgt cgtcgccggt gcgctcggcg gccaccagca acgcgcggcc catctgccag
     1741 ccgcccacca ggttgccggc cagcatcagg tacggcaccg agccggcgaa cgcggcgttg
     1801 gggctgccct tggcgttgct cgcgatgaac gccacggcgt cgacgaacgc ctcgcgcgcg
     1861 gccttcaggc gcttgccgaa ggcctgcgcg tccttgttcg acgaggcggc cagcgccttc
     1921 tccgtcgact cgatgcgcgc ggcgatcgcc ctggcggcgg tgccgccgtc gcgtgcggtc
     1981 ttgcgcccga cgaagtcgtt ggcctggatc gcggtggtgc cttcgtagat cgtcaggatc
     2041 ttcgaatcgc gcatgtactg cgcggcgccc gtctcctcga tgaagcccat gccgccgtgc
     2101 acctgcaccc ccaggctcgc gacctcgatg ctcatctcgg tggagtaggc cttgaccagc
     2161 ggcaccatga attcgtagaa cgcctggttc tcggcgcgca cggacgcatc cgggtgctcg
     2221 tgcgcggcgt cgaaggcgct cgcggccacc agcgccatcg cgcggcaggc ctcggtgtac
     2281 gcgcgcatcg tcatcagcat gcggcgcacg tcggggtggt ggatgatggg cgcggccgtc
     2341 gtcatcgagc cgtcgaccgg gcgcgactgc acgcgttcgc gcgcatattc gaccgccttc
     2401 tggtacgcgc gctcggccac cgcgatgccc tgcacgccca cgccgaagcg cgccgcgttc
     2461 atcatgatga acatgtactc caggccgcgg ttctcctggc ccacgagata gccgacggcg
     2521 ccgccgtggt cgccgaactg cagcacgcag gtcgggctcg ccttgatgcc catcttgtgc
     2581 tcgatgctca cgcagtgcgc gtcgttgcgc gcgcccagcg agccgtcggc gttgaccagg
     2641 aacttcggga cgatgaacag cgagatgccc ttgacgccct cgggcgcgcc cgcgacgcgc
     2701 gcgagcacga gatggacgat gttctcggcc atgtcgtgtt cgccccaggt gatgaagatc
     2761 ttggtgccga acaccttgta cgtgccgtcg gcctgcggct cggcccgcgt gcgcaccagc
     2821 gccaggtccg agcccgcctg gggttcggtc aggttcatcg aacccgtcca cttgcccgag
     2881 agcatgttgg gcaggtacct ggccttctgc tcgtcggagc cggccgtcag cagcgcctcg
     2941 atggcgccgt cggtcagcag cgggcacagc gcgaagctga cgttggccga gttgatcatc
     3001 tcgccgcacg cggcgccgat cagcttgggc aggccctggc cgccgaagtc ggtcgggtgc
     3061 tgcaggccct gccagccgcc ctcgccgaac tgcttgaacg cctccttgaa gccgggcgtc
     3121 gcgatgacct ggccgtcctt gaagaacgac gggttgcgat cgccctcggc gttcagcggc
     3181 gccacgacgt cctcgttgaa cctcgcgcac tcctcgagca ccgcctgcgc ggtgtcgaag
     3241 ccggcgtcct cgaacgcggg catcctggcc agttcgtcga tgccggccag ctccttcatc
     3301 acgaagagca tgtccttgac gggggcacgg taggtcatct ttgtctccac actggcaaga
     3361 aaaggggctt ttcgccgaaa taccgggccc gcgcagcgcg ggtcagccgc aggcgcaacc
     3421 cgccgttcgc cggcaccgcg acacacggga gggttcgccg ggcgcctgtg tcgccacgcc
     3481 agcgaacggc gggttacgcc tgcggctaac ccgccctacg cgcccgtgtc gttcagatcg
     3541 cgctcagagc gccttgatca tttccggaac cgcgacgaac aggtcggcca ccaggccgta
     3601 gtcggcgacc gagaagatcg gggcctcctc gtccttgttg atcgcgacga tgaccttcga
     3661 gtccttcatg ccggccaggt gctggatcgc gcccgagatg ccgcacgcga cgtacagctg
     3721 cggcgcgacg atcttgccgg tctggcccac ctgccagtcg ttcggggcgt agcccgcgtc
     3781 gacggcggcg cgcgaggcgc ccagcgcggc gcccagcttg tcggccagcg gcgtcagcac
     3841 ctcgttgaac ttctcgctgg agcccatcgc gcggccgccc gagacgatga tcttggcggc
     3901 ggtcagctcg gggcgatcga gcttggcgat ctccttgccg acgaacgtcg acttgccgcc
     3961 gtcggcgacg gcagcgatcg tttccacggc tgccgaaccg ccggtggcgg cggccgggtc
     4021 gaagccggtg gtgcgcacgg tggcgacctt gacgggctgc gagcactgca ccgtggcgat
     4081 ggcgttgccg gcgtagatcg ggcgctcgaa cgtgtcggcg gaaacgacct tgctgatgtc
     4141 gctcagctgg ccgacgtcga gcttggcggc cacgcgcggg gcggtgttct tgccgttggc
     4201 ggtcgacgga aagaagatgt gcgagtagcc cgaggccacg gccagcacct gggcggccag
     4261 gttctcggcg agggcttcgg ccagcgccgc cgaatcggcg tgcagcacct tggccacgcc
     4321 cgcgacggcg gccgccgcgg tggcgacggc accggcgttg tggccggcca cgagcacgtg
     4381 gacatcgccg cccacttgag ccgcggcggt gatggtgttg agcgtggcgc ccttgagcga
     4441 ggcgttgtcg tgttctgcga tgacgagggt ggtcatgtga tcgtgtctcc gggatgcggt
     4501 cagatgacct tcgcttcgtt cttgagcttg gcgaccagcg tcgccacgtc cggcaccttc
     4561 acgccggcac cgcgcttggg cggctcgctg accttgagcg tcttgatgcg cggcgcgacg
     4621 tccacgccca gcgcgtccgg cttgacgatc tcgagcggct tcttcttggc cttcatgatg
     4681 ttgggcagcg tcacgtagcg cggctcgttc aggcgcaggt cggtggtgac gatggccggc
     4741 agcgacagcc ggagcgtctc ggagccgccg tcgacttcgc gcgcgacggt gacggaatca
     4801 gcggtcagtt cgaccttcga ggcgaacgtg gcttggggca ggtcggccag cgcggccagc
     4861 atctggcccg tctggttgca gtcgtcgtcg atggcctgct tgcccaggat cacgaggccc
     4921 ggctgttcct tgtcgaccag cgccttgagc agcttggcca cggccagcgg ctgcagctcg
     4981 gcgtcggtct cgaccaggat gccgcggtcc gcgccgatgg ccatcgcggt gcgcagcgtc
     5041 tcctggcact gcgtgacgcc gcacgagacg gcgatgatct cggtcaccac gcccttctcc
     5101 ttcaggcgca cggcctcctc gacggcgatc tcgtcgaagg ggttcatgct catcttgacg
     5161 ttggcgatgt cgacgccagt gccgtccgac ttcacgcgaa ccttcacgtt gtagtcgacg
     5221 acgcgtttca cgggcaccag aaccttcatg acgcagaact cctcaggggc aattgacgtt
     5281 aacgtaaacg gaatacgggt tgattctagt gggccggcgc ccggcgcagc cttcttcccg
     5341 aagcaactat aagagaataa aacgaacgat cgtgctattt taggtgaaac cctccaggat
     5401 tccaatagac ttcgagccca tgtcgcgcca agccaaccct gcccccgcct cgccgtccgc
     5461 cgcgccccag ccggtgcgca tcggcatctt cgactccggc gtcggcgggc tgtcggtgat
     5521 gcaggccgtg cgcgcccgcc tgccgcacgc cgagctgctg tacgcggccg acaccgcctt
     5581 cgccccgtac ggcgaccgca ccgaggaatt cctgtgcgac cgctccgagc gcatcgcgcg
     5641 cttcctgcgg gaccagggcg cccagacgat cgtcgtcgcg tgcaacacgg ccacggccgc
     5701 cgccgtcgcc cggctgcgcg cgacctggcc cgcgctgccg gtcgtggggg tcgagcctgg
     5761 cgtgaagccc gccgtcgccc tcagcgccac gcaccgcgtc ggcgtgctcg ccaccacccg
     5821 cacgctcgcc agcgagaagt tccgccgcct ggccgaggcc cacgccggcg acgcccagct
     5881 ggtgctgcag ccctgccccg gcctggccga cgccatcgag tccgccgacg acgccgccat
     5941 cgacgtgctg gtgcagcgct actgcgcccc gctgcgcgac gccggcgccg acgtcgccgt
     6001 gctcggctgc acgcactacg tgttcgcccg cgaacggttc gagcgcgcgc tgcccggcgt
     6061 gcagctggtc gacaccgccg aagccatcgc gcgccagacg gcccgcttcg ccgacgagct
     6121 cgtcgcccgc ggcgaggccg atgccgacgc ggcgcgcccg tccgtcctgc acgcgtggag
     6181 cagcggctcg gcgcacgtgc tcgcatcctt cgtgcagcgc tggctggggc tgcgggtcga
     6241 cgtgcgcgag atgcgctgct gagcgcgccg ttgtccacgc ccgcgcccgc cgccgcgcgc
     6301 gtcacgcgcg agcgcccgtc cacgcagatg cggcgcctgc ggcatgcgcc gctgggccgc
     6361 tgcatgtact gcctcgcgcc cggcggcccg ggcatcgtgc tcacggagga acacctggtg
     6421 ccgcgcgccc ttggcggccg cctgacgctg cgcgacgccg tctgcgagcc ctgccgtcgc
     6481 ctcaccggcc gccacgagca gctgacgctg gaccgcgagt tcgcggtacc ccggacgctg
     6541 ctcgcgatga aacggcgccg ggcgcgcgcg aagggcccgg gccgcctgcc ggccgtggtg
     6601 ctcgaaggcg gcgggtcacc cgtgacgctg cccgccgacg ccttcccccg cacgttcgcg
     6661 ctgcccgcgt tcgagcgcgc cgggctgctt gctggcatcg atcgcgccga cgtgccggcc
     6721 cacgtcgact tcgtcgcgtg cacgctggac ctgggcagca tcgcgcgccg caccgccgcc
     6781 gcgccgccgg ccctgcccga cccggcggcc tacgcgcatt cgatcgccaa gtgggcctac
     6841 gcgctggccg tggccgagcg cggccccgcc tgctgcgaca cggagccgct gcgcgacctg
     6901 gtgctcggac gccgcgacga cgtgttcgag ttcgtcggat cggcatccga acgcgcaccg
     6961 gcgtcgcgcg aatggctgca cgacgtcgcc ttccacgagc aggacggctg gctcgtcgcg
     7021 acgctgtcgt tgttcgcatc ggcaggcatg gcgccgtatg aagtcgtcat cgggcgcctg
     7081 gccggccgct gaaggccgcc gctcgtcgca gcaggcccca cggcgcgcat cgattggcct
     7141 agcatcggcc atccccttca aggagacccg cgatgcgcat tcgccttctg gccgccgcca
     7201 ccctgtccgc cgccctgctg gcgccgccca cgccgtcgcg cgccgccgac gcgccgctca
     7261 ccggcgacac ggccgcggcc gctcaggtct ggtccacctt cgagcactgg ctgaaggcct
     7321 acgaggcggg cgacctggac gggatcatgg cgatcttcga ccgggacgtg atcttcgaat
     7381 tccagggcgg ccccgacgcc accgccgaca tgttgcggcg cgactacgcc gacgacctca
     7441 aggcccgcgc cgcgggcacg cgctgggtgc cgcgcgtgga ggaggtccac gccgacggca
     7501 acacggcgtt cgtgcgctcc atgtgggagc tgcacgtcgg cgacaaggtg acccagcgca
     7561 atcgcagcat ggacgtgttc cagcgccgcg acgggcactg gaccatcctg cgc
//
LOCUS       DATCRO010000217         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_992387, whole genome shotgun sequence.
ACCESSION   DATCRO010000217 DATCRO010000000
VERSION     DATCRO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992387"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VIN75_05910"
     CDS             complement(<1..851)
                     /locus_tag="VIN75_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973733.1"
                     /translation="MLNSETLEVAVGMVFLFLLMSLICTAIKEWIEALLKWRAMDLER
                     AMRTLLDDDDGSTTQALYTHPIIYSLFQGTYDAAQLRSSWFAMGTGADKHMRLGARRN
                     LPSYIPTAHFATAFIDYVARGPAVPGAAASTAPAAPLTIDLLRQQAALLPEHLSRTVI
                     AGIDYANGDIAKVRKAVEQWFDGAMDRASGWYKRRTQALLFTVGLLTAVVLNVDALHI
                     LHRLTHDKTFRDVVVSRAAVAKAPASASAGDELASITLARGELDAVTMPIGWESPTTA
                     GAETQLWP"
     gene            999..1496
                     /locus_tag="VIN75_05915"
     CDS             999..1496
                     /locus_tag="VIN75_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8973734.1"
                     /translation="MDAITLATLAAFPDQLEAHFAAFPPEFRHWAPASWDGVPSEALT
                     ALEQLCHVRDIEIDGYQLRIRRTLAEDEPLLASIDTDALVRERAYARDEPARVLASFR
                     AARAATMEMISGLSDTQLARTAVFEGYGPLTLRSLIHYLCSHDQQHLAGLQWVLGKAH
                     ASRPG"
     gene            1761..2891
                     /locus_tag="VIN75_05920"
     CDS             1761..2891
                     /locus_tag="VIN75_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEY8973735.1"
                     /translation="MKLRTFVVAAAAALLGHAAQAQSWQLVWSDEFNGSISPDWVFET
                     GGGGWGNNELEYYRKENATIENGNLVITAKKEDFGGYHYTSARMTTQGTRSWKYGRME
                     ARIKLPAVQGTWPAWWMLGNNISSVGWPASGETDIMEQINTGGTIYGSVHWQAANGSQ
                     ADYSNNIGTTPTDYHVYAVEWDQDYIKWFVDGTQYNQFQITNGVGNTQAFQNPFFLLL
                     NMAVGGNWPGFSVNDAALPAKMYVDYVRVYQKAAPVNIHIEAENWAVMSGVQTEACSE
                     GGQDVGWIDANDWIVWNVNIPAAGTYTVSYRVASLNGGGVIQLEKAGGSPVYGSVAVP
                     KTGGWQNWTTISHQVTLPAGQQQIAVKALAGGYNINWLNITK"
     gene            complement(2934..3410)
                     /locus_tag="VIN75_05925"
     CDS             complement(2934..3410)
                     /locus_tag="VIN75_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8973736.1"
                     /translation="MTSDETFDGGCDCRAVRYRLRSRPMFVHCCHCRWCQRESGASFA
                     LNALIEADRVELLAGPVDIVDTPSHSGNGQRIARCPTCRIALWSHYSSPLVSFVRVGT
                     LDDPDRFPPDIHIFTSSKQPWVQLPGDVPVVPEYYRSAEMWPADALARRAALFPSK"
     gene            complement(3556..>4168)
                     /locus_tag="VIN75_05930"
     CDS             complement(3556..>4168)
                     /locus_tag="VIN75_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8973737.1"
                     /translation="HASIADGVIAMIRGALAELNVGDPARLSTDVGPVIDDEAYANVS
                     RNVARLKKEAKLLGETPLASNFPRLVTPVAFELGRIADLKEEIFGPVLHVVRWNGDVD
                     TVMHEINALGYGLTLGIQTRIDSRAQRLARLARVGNVYVNRNMIGAVVGVQPFGGEGL
                     SGTGPKAGGPHYLFRFCAEQTLTINTAAAGGNAALLAGESGGH"
BASE COUNT          678 a         1453 c         1428 g          609 t
ORIGIN      
        1 ggccacagct gcgtctcggc gcccgcggtg gtcggcgact cccagccgat gggcatggtg
       61 acggcgtcga gttcgccgcg cgccagcgtg atcgacgcga gctcgtcgcc cgcgctcgcc
      121 gaggccggcg ccttggccac ggcggcgcgg ctgacgacga cgtcgcggaa ggtcttgtcg
      181 tgcgtcaggc ggtgcaggat gtgcagcgcg tcgacattga gcacgaccgc ggtcagcagc
      241 cccaccgtga acagcagcgc ctgcgtgcgc cgcttgtacc agcccgaggc gcggtccatc
      301 gcgccgtcga accactgctc caccgccttg cgcaccttgg cgatgtcgcc gttggcgtag
      361 tcgatgccgg cgatcaccgt gcgcgaaagg tgctcgggca gcagggcggc ctgctggcgc
      421 agcaggtcga tggtcagggg ggcggccggc gccgtggatg ccgcggcgcc gggcacggcc
      481 gggccgcggg cgacgtagtc tatgaacgcg gtcgcgaagt gcgcggtcgg gatgtaggac
      541 ggcaggttgc ggcgcgcgcc gagccgcatg tgcttgtcgg cgccggtgcc catcgcgaac
      601 cagctgctgc gcagttgcgc ggcgtcgtag gtgccctgga acagcgagta gatgatcgga
      661 tgcgtgtaca gcgcctgcgt ggtgctgccg tcgtcgtcat ccagcagcgt gcgcatcgcg
      721 cgctccaggt ccatcgcgcg ccatttcagc agcgcctcga tccattcctt gatcgccgtg
      781 cagatcaacg acatcagcag gaacaggaac accattccga cggcgacttc caacgtttcc
      841 gaattcagca tggctctcct ccgcccgggc aaggcggcaa gcctacgacc gcgggagcgg
      901 cctggcgtcc gtcgaattgg ggggcgcggg cgcggccttc aggtcgcggc aaagtcccgg
      961 ctcgcgccat gagcgggcgc ttgcgccaag atcgcgcgat ggatgccatc acgctcgcca
     1021 ccctcgccgc cttccccgac cagctcgaag cgcatttcgc cgccttcccg cccgagttcc
     1081 gccactgggc gccggcgtcc tgggacggcg ttcccagcga ggcgctcacg gcgctggaac
     1141 agctttgcca tgtgcgcgac atcgagatcg acggctacca gctgcgcatc cgccgcacgc
     1201 tcgccgagga cgagccgctg ctcgcgtcga tcgacaccga cgccctcgtg cgcgagcgcg
     1261 cctacgcgcg tgacgagccc gcgcgcgtgc tcgcgagctt ccgcgccgcg cgcgccgcga
     1321 cgatggagat gatctccggc ctgtccgaca cgcagctcgc gcgcaccgcg gtcttcgaag
     1381 gctacgggcc gctcacgctg cgcagcctga tccactacct gtgcagccac gaccagcagc
     1441 acctggccgg cctgcagtgg gtgcttggca aggcgcacgc gtcgcgtccg ggctgaggtt
     1501 gcgcccggcc gccgcgccgt ctgtccggcg tgtgcactgc agcatttccg acgcgcgccg
     1561 aaggattgcc tgcccgagga ggtcggcgcg cccacggctc agggaaatcc ccgccgtgaa
     1621 atcgccggat ccgacgtgcc gtacccagtt tcagcgatgc gtcatgaaag cgctttcgtt
     1681 agcatcggcg cgcgcgcggc ggaaccggtt ccatcgtgcg cgcccgaaat ccacgaaaac
     1741 attcgcatga ggcaagcatg atgaaactcc gcacctttgt cgtggcggcg gccgcggcgc
     1801 tgctcggtca cgcggcccag gcgcagtcct ggcagctcgt ctggtccgac gagttcaacg
     1861 gctccatcag ccccgactgg gtcttcgaga ccggtggcgg cggctggggc aacaacgagc
     1921 tcgagtacta ccgcaaggag aacgcgacca tcgagaacgg gaacctcgtg atcaccgcca
     1981 agaaggaaga cttcggcggc tatcactaca cctcggcgcg catgacgacg cagggcacgc
     2041 gcagctggaa gtacggccgc atggaggcgc gcatcaagct gccggccgtg cagggcacct
     2101 ggccggcctg gtggatgctg ggcaacaaca tctcctccgt gggctggccg gcgtcgggcg
     2161 agaccgacat catggagcag atcaacaccg gcggcacgat ctacggcagc gtgcactggc
     2221 aggccgccaa cggctcgcag gccgactaca gcaacaacat cggcaccacg cccaccgact
     2281 atcacgtgta cgcggtcgaa tgggaccagg actacatcaa gtggttcgtc gacggcacgc
     2341 agtacaacca gttccagatc accaacggcg tgggcaacac gcaggcgttc cagaatccgt
     2401 tcttcctgct gctgaacatg gccgtgggcg gcaactggcc gggcttctcg gtcaacgacg
     2461 ccgcgctgcc ggccaagatg tacgtcgact acgtgcgcgt ctaccagaag gccgcgccgg
     2521 tcaacatcca catcgaggcc gagaactggg cggtgatgag cggcgtccag accgaggcct
     2581 gctccgaggg cgggcaggac gtgggctgga tcgacgccaa cgactggatc gtctggaacg
     2641 tcaacatccc cgcggcaggc acctacacgg tctcgtaccg cgtggcgagc ctgaacggcg
     2701 gcggcgtgat ccagctcgag aaggccggcg gcagcccggt ctacggcagc gtggcggtgc
     2761 ccaagaccgg cggctggcag aactggacga cgatctcgca ccaggtgacg ctgcccgcgg
     2821 ggcagcagca gatcgccgtc aaggcgctgg ccggcggcta caacatcaac tggctcaaca
     2881 tcacgaaatg agcgccggtt gatcggcgag acggggcctg cgcggccccg tcctcatttc
     2941 gacgggaaca gcgccgcgcg ccgcgccagc gcgtcggccg gccacatctc ggcgctgcgg
     3001 tagtactcgg gcacgaccgg cacgtcgccg ggcagctgca cccacggctg cttggacgac
     3061 gtgaagatgt ggatgtcggg cgggaagcga tcgggatcgt cgagcgtgcc cacgcgcacg
     3121 aagctcacca gcgggctgct gtagtggctc cacaacgcga tgcgacacgt gggacagcgc
     3181 gcgatgcgct ggccgttgcc gctgtgggac ggcgtgtcga cgatgtccac cggccccgcc
     3241 agcagctcga cccggtcggc ctcgatcagg gcgttcaacg cgaacgacgc gcccgactcg
     3301 cgctggcacc atcggcagtg gcagcagtgc acgaacatcg gccgcgagcg caggcggtag
     3361 cgcacggcgc ggcagtcgca gccgccgtcg aaggtctcgt cgctggtcat ggaagaagtc
     3421 ctccagtcag tgtcatcctg cgcgtagggc gggttagccg aaggcgcaac ccgccaccga
     3481 cgagccatcg caacgccggc gaacggcggg ttacgccttc ggctaacccg ccctacggcc
     3541 gacaggggcg ttctctcaat gtccgcccga ttccccggcc agcaacgccg cattgccacc
     3601 cgcggccgcg gtgttgatcg tcagcgtctg ctccgcgcag aagcggaaca ggtagtgcgg
     3661 gccgccggcc ttcgggcccg tgcccgacag gccctcgccg ccgaacggct gcacgcccac
     3721 gaccgcgccg atcatgttgc ggttgacgta gacgttgccg acccttgcga ggcgtgcgag
     3781 gcgctgcgcg cggctgtcga tccgcgtctg gatgcccagc gtcaggccgt agcccagcgc
     3841 gttgatctcg tgcatcaccg tgtcgacgtc gccgttccag cgcaccacgt gcagcaccgg
     3901 gccgaagatc tcctccttga ggtcggcgat cctgcccagc tcgaaggcca ccggcgtgac
     3961 caggcgcggg aagttcgagg cgagcggcgt ctcgcccagc agcttggcct ccttcttcag
     4021 ccgcgccacg ttgcgcgaga cgttcgcgta cgcctcgtcg tcgatcaccg ggccgacgtc
     4081 ggtcgacagg cgcgccggat cgccgacgtt gagctcggcg agcgcgccgc ggatcatcgc
     4141 gatcacgcca tcggcgatcg acgcgtgc
//
LOCUS       DATCRO010000218         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_879947, whole genome shotgun sequence.
ACCESSION   DATCRO010000218 DATCRO010000000
VERSION     DATCRO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879947"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VIN75_05935"
     CDS             <1..1077
                     /locus_tag="VIN75_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEY8973738.1"
                     /translation="GMAQATWEGFKAAVPDERPFILSRSAAAGSSRWTALWTGDNWSN
                     WHHLRMSIPCTLNLALSGIPFNGPDVPGFGGHADAELAVAWYKAGMLFPFLRNHSVKG
                     SVAQEPWQFGPQALEVIRHCVRLRYKLLPYLYQLWIAQARDGAAVMRPLFHDFDNADG
                     LDLDRIDDQFLVGPALMQAPLLHQGQQHRTVALPGAGRWLDACNGRFVAAGRSVHVRS
                     DAASTPLYVREAALLPMQPGVRTTQANDLADIELHVILGRGCSDEAVLDYAFDDGLSD
                     AHARGGRGVVRFRATRSGDEIALRVETVQAWKPLRVSVVGYDGAATARIVRASGEQVQ
                     ALVGDTWRFTGQPLAVGRGAGFVA"
     gene            complement(1231..1536)
                     /locus_tag="VIN75_05940"
     CDS             complement(1231..1536)
                     /locus_tag="VIN75_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973739.1"
                     /translation="MNKFHAVAAAAALAAGLLTATSSMATTAFDNKMANVIKTVKADP
                     SYKAIPLDSSADRQWFFAQCEALYGKKITKEQFVADGAKQFPGYEDSFSRLADQLAS"
     gene            2290..2595
                     /locus_tag="VIN75_05945"
     CDS             2290..2595
                     /locus_tag="VIN75_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8973740.1"
                     /translation="MKSILVLVPALACAMPAMAGIEMAQKKNCLSCHMIDQKLVGPAW
                     RDVAVKYAGREDAVSILSTKVQKGGTGVWGTAVMPPNPVTPDEARELVTWILTLKKN"
     gene            2679..3953
                     /locus_tag="VIN75_05950"
     CDS             2679..3953
                     /locus_tag="VIN75_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973741.1"
                     /translation="MNDPDSNGRRRWARAATVLLLALASSLAHAVPSFARQTGQPCAM
                     CHVGGFGPQLTDYGIQFKLTGYTTTKGGDWIPPVAGMVMAGLTHTSKAQDASTLDGHS
                     LTGSLKGNDNLTLDQVSLFVSGRISDNVGIFSQITYDGIAQHTSIDNVDLRAAATTQL
                     GDHLAVLGVSVNNNPGVQDPFNTLPAWLFPYISSNVAPAPSAGTILDEAFGQKATGVV
                     GYAQLDGMWYGELGTYRMQTPTLQKDFGIAAGDRDPGALRSPLYARLAARKSLESSSM
                     EAGVVLFKTGYQPDRSVPDTAHIRDLGFDASYRWTSPGGDIVTVLGNVIKETQAGADL
                     TEYSVNASYFWDRSWGATLQRFGVNAPGLGSRGTRLQFDWTPFGKTPPSGGIDANMRF
                     GLQLTAYDKLDGVTGSAATDANSLYLFAWLAF"
     gene            complement(3970..>4312)
                     /locus_tag="VIN75_05955"
     CDS             complement(3970..>4312)
                     /locus_tag="VIN75_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973742.1"
                     /translation="IDRSAQRGGCLMGALRWLTLDVSDNDEGVVTIEALASTDAAGHA
                     FALAEARSLLDWAWQAFPDTHGPADEGGQWDHDLQSGADADGWHSVALTLTGTRAFAE
                     AFELEFDVPGE"
BASE COUNT          669 a         1498 c         1488 g          657 t
ORIGIN      
        1 ggcatggcgc aggccacctg ggaaggcttc aaggccgcgg tgcccgacga gcgcccgttc
       61 atcctctcgc gcagcgcggc cgccggatcg agccggtgga cggcgctgtg gacgggcgac
      121 aactggagca actggcacca cctgcgcatg agcatcccgt gcacgctcaa cctcgcgttg
      181 tcgggcatcc cgttcaacgg cccggacgtg ccgggcttcg gcggccatgc ggacgccgag
      241 ctcgccgtgg cctggtacaa ggccggcatg ctgttcccgt tcctgcgcaa ccactcggtc
      301 aagggctcgg tggcgcagga gccgtggcag ttcgggccgc aggcgctcga ggtcatccgc
      361 cactgcgtgc ggctgcgcta caagctgctg ccctacctgt accagctgtg gatcgcgcag
      421 gcacgcgacg gcgcggccgt gatgcgcccg ctgttccacg acttcgacaa cgccgacggg
      481 ctcgacctcg accgcatcga cgaccagttc ctcgtcggcc cggcgctgat gcaggcgccg
      541 ctgctgcacc agggccagca gcatcgcacc gtggcgctgc cgggcgccgg gcgctggctg
      601 gacgcgtgca acgggcgctt cgtggccgcg ggccgcagcg tgcacgtgcg cagcgacgcc
      661 gcgtcgacgc cgctgtacgt gcgcgaggct gcgctgctgc ccatgcagcc cggcgtgcgc
      721 accacgcagg ccaacgatct ggccgacatc gagctgcacg tgatcctggg acgcggctgc
      781 agcgatgaag cggtgctgga ctatgcgttc gacgacggac tgagcgatgc gcacgcgcgc
      841 ggcgggcgcg gcgtcgtccg cttccgcgcg acgcggtcgg gcgacgagat cgcgctgcgc
      901 gtcgagaccg tgcaggcctg gaagccgttg cgcgtgagcg tcgtcggcta cgacggcgcg
      961 gccacggcgc gcatcgtgcg ggcgtcgggc gagcaggtgc aggcgttggt gggcgacacg
     1021 tggaggttca cgggccagcc gctggctgtc gggcgcgggg ccggtttcgt tgcctgatcc
     1081 ggcatcggca tcggcttgcc cgcgcaaggc gggtcacgcc tgcggccaac ccccctacgg
     1141 atgcatggcg cctgcatgca tgacgagcga aagcgacgac ggcaccgatc gatgaacgag
     1201 cggtgccgtc gcgcgaggac gggcccggga tcaggaggcg agctggtcgg ccaggcggct
     1261 gaacgaatcc tcgtagcccg ggaactgctt ggcgccatcg gcgacgaact gttccttcgt
     1321 gatcttcttg ccgtacagcg cttcgcactg ggcgaagaac cactggcggt ccgcgctgga
     1381 gtccagcggg atggccttgt agctcggatc ggccttgacg gtcttgatca cgttggccat
     1441 cttgttgtcg aaggcggtgg tggccatcga cgaggtggcg gtcagcaggc ccgcggcgag
     1501 cgcggcggca gcggcgacgg catggaactt gttcatggag ttctccagat tcgagtaggg
     1561 gtgtgtcccg cgcgccggcc gcccgcgcga ggacgaaatc atcccgacac ggcgatgggg
     1621 agatcgctcg tgcggtgctt gcgcggcaac gcacgtctgc attgccgatg gacgaaagtc
     1681 taggtcgcga gggcgcgcga gtcactgcag ttcgtgccct caaagtcgca cttcgtcgca
     1741 cgggccgaaa cccccctgcg tatccgccat ggtggcgatg ccgcgagggc gtggacggtc
     1801 gatcgtccag ccggcgagcg tgtccttgcc ggctccgccg gcgccgagat cggccgcagc
     1861 cgcattcgcc tgctcgacgg cttcgaggac ttgcgaacga tcggcggttc cgtgtgtttc
     1921 atgcgacacc ggttccgatc tgacagcgct gactcggatg tttccgacac ccagttcatg
     1981 gggggtgatc ggccctctgg cgacagcctg agttcagcct cgatcgacat ccccggcgcg
     2041 tccctctgga agaggtctcc tgcatgcggc aaggacgcat ttttcgcatc cctggaaccg
     2101 ggtagagatc tgatcagacg cttctgaaaa catccgctcc ctttaccgtt ccttaagaca
     2161 tgtggctaac ctgcaagcga gtcggccgtg tccgacggcg attgaaccgc ccggctcgaa
     2221 gagggacttt acggcgagct gcaacgcgaa cgctgcgcgt gagtgcccgc cgcaacatga
     2281 ggaggtgcca tgaaatcgat tctcgtgctc gtgcccgcac tggcctgcgc gatgcctgcc
     2341 atggccggca tcgagatggc acagaagaag aactgcctga gctgccacat gatcgaccag
     2401 aagctcgtcg ggcccgcgtg gcgcgacgtc gccgtgaaat acgccggccg cgaagacgcg
     2461 gtgtccatcc tgtcgaccaa ggtccagaag ggcggcaccg gcgtctgggg cacggccgtg
     2521 atgccgccca acccggtcac gcccgacgag gcgcgcgagc tcgtgacctg gatcctcacg
     2581 ctcaagaaga actgaccctc cgcgaccccg gaggtcgatc gtgcgcgggc tcgccgagct
     2641 cgtgcggcct gaccacgtcc ttgcaaggag acccgctcat gaatgatccc gactcgaacg
     2701 gacggcgccg ctgggcccgc gccgccaccg tgctgctgct ggccctggcc agctcgctcg
     2761 cgcacgccgt gcccagcttc gcgcggcaga ccggtcaacc gtgcgcgatg tgccatgtcg
     2821 gcggcttcgg cccgcagctg accgactacg gcatccagtt caagctgacc ggctacacca
     2881 ccaccaaggg cggcgactgg atcccgcccg tcgcgggcat ggtgatggcg gggctcaccc
     2941 acaccagcaa ggcgcaggac gcgagcacgc tcgacggcca ttcgctgacg ggctcgctca
     3001 agggcaacga caacctgacg ctcgaccagg tctcgctgtt cgtgtcgggc cgcatcagcg
     3061 acaacgtggg catcttctcg cagatcacct acgacggcat cgcccagcac acctcgatcg
     3121 acaacgtcga cctgcgggcc gccgccacca cgcagctcgg cgaccacctg gccgtgctgg
     3181 gcgtctcggt caacaacaac ccgggcgtgc aggatccgtt caacacgctg ccggcgtggc
     3241 tgttcccgta catctcgtcc aacgtggcgc cggcgccgag cgcgggcacc atcctcgacg
     3301 aggcgttcgg ccagaaggcg accggcgtcg tcggctacgc ccagctcgac gggatgtggt
     3361 acggcgagct cggcacctac cgcatgcaga cgccgacgct gcagaaggac ttcggcatcg
     3421 ccgcgggcga ccgcgacccg ggcgcgctgc gatcgccgct gtacgcgcgc ctcgcagcgc
     3481 gcaagagcct ggagtcgtcg tcgatggagg cgggcgtcgt gctgttcaag acgggctacc
     3541 agcccgaccg ctccgtgccc gacaccgcgc acatccgcga cctcggcttc gatgccagct
     3601 accgctggac gagcccgggg ggcgacatcg tcaccgtgct cggcaacgtc atcaaggaga
     3661 cccaggccgg cgccgacctg acggagtaca gcgtcaacgc gtcgtacttc tgggaccggt
     3721 cgtggggcgc gacgctgcag cgcttcggcg tcaacgcgcc cgggctgggc tcgcgcggca
     3781 cgcgcctgca gttcgactgg acccccttcg gcaagacccc gcccagcggc ggcatcgacg
     3841 ccaacatgcg gttcggactg caactgacgg cctacgacaa gctggacggc gtgaccggat
     3901 cggcggccac cgacgccaac agcctgtacc tgttcgcgtg gctggcgttc tgatgccgcg
     3961 tgccgagcgc tactcgcccg gcacgtcgaa ctcgagttcg aaggcctcgg cgaacgcccg
     4021 cgtcccggtc agcgtcagcg cgaccgaatg ccacccgtcg gcgtcggcgc ccgactgcag
     4081 gtcgtggtcc cactgaccgc cctcgtcggc ggggccgtgc gtgtcgggaa aggcctgcca
     4141 ggcccagtcg aggaggctgc gcgcctcggc cagcgcgaac gcgtggcccg cggcgtccgt
     4201 ggacgcgagc gcctcgatcg tgaccacgcc ctcgtcgttg tcgctgacgt cgagcgtgag
     4261 ccagcgcagc gcgcccatca ggcaacctcc gcgctgcgcg ctgcggtcga tt
//
LOCUS       DATCRO010000219         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_317376, whole genome shotgun sequence.
ACCESSION   DATCRO010000219 DATCRO010000000
VERSION     DATCRO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317376"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VIN75_05960"
     CDS             <1..506
                     /locus_tag="VIN75_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY8973743.1"
                     /translation="ELLADPKERAEHLMLIDLARNDIGRIAQTGSVKVTDAFVVERYS
                     HVMHIVSNVEGLLKPGMTNMDVLRATFPAGTLTGAPKIRAMEIIDELEPVKRGIYGGA
                     CGYLSYAGDMDVAIAIRTGIVQNNTLYVQAAAGVVADSIPEMEWRETEAKARALLRAA
                     ELVEEGF"
     gene            595..1776
                     /locus_tag="VIN75_05965"
     CDS             595..1776
                     /locus_tag="VIN75_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8973744.1"
                     /translation="MSKFSSCLSRLAVAIAPLLLMAGCAVPTHPLAAASPELFDDAHF
                     GPPEETIDPDDVFRMSPEMEAYLDKVIMPMARARGVREALTEALYTRSKLMLEYDSST
                     TRTASQAFEARQGNCLSLIIMTGAFANAMGLKVTFQQVATEDMWSRSGGMYFMSGHVN
                     IAFQHRFDKSVIYTIDFMPAVNDGYFTRPISQETVLAMYMNNRAAEAMVRGKLDDAYW
                     RIRQAVKLDPQFRSAYNTLGVIYLRHGDAAHAEQVLSYVLQGEPGNPRVLANYAEALT
                     QLGRTAEAKAVQARLAVLEPVPPFYWFVQGQAALHKGDFMKARELFLKEIDRDPDYHE
                     FHYALAVADFGLNRLDEAKAEMSLAMSDAVKRSDHDLYAAKLDKLKAWHPNAAAPSLV
                     Q"
     gene            complement(1798..2979)
                     /locus_tag="VIN75_05970"
     CDS             complement(1798..2979)
                     /locus_tag="VIN75_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973745.1"
                     /translation="MLPPDDSPDGRPAAAPPLAGALRARAQPRRRARRAPDDAESAPP
                     PSIEEQLDRAEICSRSGRIDDAIDICNALRPSLVAGGDPRSVGWCDFVLALSHLNAGR
                     AKEAVIAGYRAIGKLEPGMKLMRGLTVLASALARTGDAGGALELLERAKRLLAQINSP
                     RDRCLFWVNCGSTFHALGDMEQAVKHSLLADGLLAEFDDPYLHAAVKMNLLVQRLILA
                     VAGCHGVETPELTELLAAASAMVERLVAAGQHYSVAKGSEDIADAYIALGRYEKAREA
                     LQVGVKSADAAKAGPDRGILELRLGRIERLAGQYRRAQAHIGLALELLAESALLKELA
                     EAHLENSLLNEERQHWRAALDSYRESAQIRERLLIAQSDARVHALEVRLELVRGGSGD
                     R"
     gene            3301..3891
                     /locus_tag="VIN75_05975"
     CDS             3301..3891
                     /locus_tag="VIN75_05975"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY8973746.1"
                     /translation="MLLMIDNYDSFTFNLVQYFGELGQDVRVVRNDEISVEEIGAMKP
                     DHLVLSPGPCSPAEAGVCVPAIREYLGKLPILGVCLGHQSIGAALGGRIIRARRQMHG
                     KASTLTTDGEGVYKDLPREFSVIRYHSLVIEQETCPAELIVTSRSEDGEIMGVRHRDF
                     VGSRTPMEGVQFHPESILSEHGHAMLRNFLHLSTLQ"
     gene            3985..>4813
                     /gene="ltaE"
                     /locus_tag="VIN75_05980"
     CDS             3985..>4813
                     /gene="ltaE"
                     /locus_tag="VIN75_05980"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY8973747.1"
                     /translation="MIDLRSDTVTRPTPAMRDAMFAAPLGDDVFGDDPSVNALQERIA
                     ALLGKEAALFMPTGTQSNLAALMSHCQRGDEYLVGQMAHTYRWEGGGAAVLGSIQPQP
                     IAHQADGTLALADIEAAIKPDDAHFARTRLLALENTFGGQPMAMSYLRDATALARRHG
                     LATHLDGARVFNAAVVQAQAGGSDPYTEVRAIADLFDSVSVCFSKGLGAPVGSALVGS
                     RELIGRARRVRKMAGGGMRQAGLLAAAALHALDHHVERLAEDHALARRLAEGLAGVPG
                     "
BASE COUNT          748 a         1731 c         1680 g          654 t
ORIGIN      
        1 aggaactgct ggccgacccg aaggagcgcg ccgagcacct gatgctgatc gacctggcgc
       61 gcaacgacat cggccgcatc gcccagaccg gcagcgtgaa ggtgaccgac gcgttcgtcg
      121 tcgagcgcta ttcgcacgtg atgcacatcg tcagcaacgt cgagggcctg ctcaagccgg
      181 gcatgaccaa catggacgtg ctgcgcgcca ccttcccggc cggcacgctc accggcgcgc
      241 ccaagatccg cgcgatggag atcatcgacg agctcgagcc ggtcaagcgc ggcatctacg
      301 gcggcgcctg cggctacctg agctacgcgg gcgacatgga cgtggccatc gccatccgga
      361 cggggatcgt gcaaaacaac acgctttacg tgcaggcggc cgccggcgtc gtggcagatt
      421 cgatcccgga gatggaatgg cgcgagaccg aggccaaggc gcgtgcgctg ctgcgcgcgg
      481 ccgagctggt cgaggaagga ttctgagcag gcttgcaagg ccccgcggaa ggcgcatagt
      541 tcacatcggg acccgtgccc cgccaggcgc gggtggccag tcgaggagtc cgtcatgtcg
      601 aagttcagtt catgcctgtc ccgcctggcg gtcgccatcg cgccgctgct gctgatggcc
      661 ggttgcgccg tccccacgca cccgctggcg gccgcgtcgc ccgagctgtt cgacgacgcg
      721 cacttcggcc cccccgagga gacgatcgat ccagacgacg tcttccggat gtcgccggag
      781 atggaggcgt acctcgacaa ggtcatcatg ccgatggcgc gcgcccgcgg cgtccgcgag
      841 gcgctgaccg aggcgctgta cacgcgcagc aagctgatgc tcgagtacga ctccagcacc
      901 acgcgcacgg cgtcgcaggc cttcgaggcg cgccagggca actgcctgtc gctgatcatc
      961 atgaccggcg cgttcgccaa cgcgatgggc ctgaaggtga ccttccagca ggtggccacg
     1021 gaagacatgt ggagccgctc gggcggcatg tacttcatga gcggccacgt gaacatcgcg
     1081 ttccagcacc gcttcgacaa gagcgtcatc tacacgatcg acttcatgcc ggccgtcaac
     1141 gacggctatt tcacgcggcc gatctcgcag gagacggtgc tcgcgatgta catgaacaac
     1201 cgcgccgccg aggcgatggt acgcggcaag ctcgacgacg cctactggcg catccgccag
     1261 gcggtcaagc tcgatccgca gttccgcagc gcctacaaca cgctgggcgt gatctacctg
     1321 cgccacggcg acgccgcgca cgccgagcag gtcctcagct acgtgctcca gggcgagccc
     1381 ggcaacccgc gcgtgctggc caactacgcc gaggcgctga cccagctggg ccgcacggcc
     1441 gaggccaagg ccgtgcaggc gcggctggcg gtgctcgagc cggtgccccc gttctactgg
     1501 ttcgtgcagg gccaggcggc gctgcacaag ggcgacttca tgaaggcgcg cgagctgttc
     1561 ctcaaggaga tcgaccgcga cccggactac cacgagttcc actacgccct cgcggtggcc
     1621 gacttcggcc tgaaccgcct ggacgaggcc aaggccgaga tgtcgctggc gatgagcgac
     1681 gccgtcaagc gcagcgacca cgacctgtac gccgccaagc tcgacaagct caaggcctgg
     1741 catccgaacg ccgcggcgcc gtcgctggtg cagtgaggat gcgcgcccgg cgcgcactca
     1801 gcggtcgccg gagccgccgc gcaccagctc cagccgcacc tccagcgcgt ggacgcgggc
     1861 gtccgactgc gcgatcagca ggcgctcgcg gatctgcgcc gactcgcggt agctgtccag
     1921 cgccgcgcgc cagtgctggc gctcctcgtt gagcaggctg ttctccaggt gcgcctcggc
     1981 cagctccttg agcagcgcgc tctcggccag cagctccagc gcgagcccga tgtgcgcctg
     2041 ggcgcgccgg tattgcccgg cgaggcgctc gatgcggccc aggcgcagct cgaggatgcc
     2101 gcggtcgggc ccggccttgg cggcgtcggc cgatttcacg ccgacctgca gcgcctcgcg
     2161 cgccttctcg taccggccca gcgcgatgta ggcgtcggcg atgtcctcgc tgcccttggc
     2221 caccgagtaa tgctggcccg ccgcgaccag gcgctcgacc atcgccgagg ccgcggccag
     2281 cagctcggtc agctcgggcg tctccacgcc gtggcagccg gccaccgcca ggatcaggcg
     2341 ctgcaccagc aggttcatct tgacggccgc gtgcaggtac gggtcgtcga actcggcgag
     2401 caggccgtcg gccagcagcg aatgcttgac cgcctgctcc atgtcgccga gcgcgtggaa
     2461 ggtgctgccg cagttgaccc agaacaggca ccggtcgcgc gggctgttga tctgcgccag
     2521 cagccgcttg gcgcgctcca gcaactcgag cgcgccgccc gcgtcgccgg tgcgtgcgag
     2581 cgccgaggcc agcaccgtca ggccgcgcat cagcttcatg cccggctcga gcttgccgat
     2641 cgcgcggtag ccggcgatca ccgcctcctt ggcgcggccg gcgttcaggt ggctcagcgc
     2701 cagcacgaag tcgcaccagc cgacgctgcg cgggtcgccg ccggccacca gcgacgggcg
     2761 cagggcgttg cagatgtcga tggcatcgtc gatgcggccc gagcgcgagc agatctccgc
     2821 gcggtcgagc tgttcctcga tggagggagg cggtgcggac tcggcgtcgt cgggcgcgcg
     2881 gcgggcgcgc cggcgcggct gcgcacgggc gcgcagcgcg ccggcgagcg gcggcgcggc
     2941 ggcgggcctg ccgtcggggg aatcgtctgg cggaagcatc gtgccatcgt agcgagcgca
     3001 cggccggcgg gcgatccggg cagaccgcaa tcgtcaccga agtcacgctt gcaacgggaa
     3061 gaaggccggc gcgccgcttt cgcatgggag gcgcttagaa tggtccggac atgaagcagc
     3121 tctcgaccat cttccacacc gggcattggc gctggtgaag atcacggccc ggtcgtgccg
     3181 ccccggcgcg cgcccgttga cccccgccgc gggctcccgc ggccgacgag agacccccga
     3241 tcgaccgtgc cgcgccttgc ggagtccccg agtcgatccc agccagacac aggaacgctc
     3301 atgcttctga tgatcgacaa ctacgattcc ttcacgttca acctcgtgca gtacttcggc
     3361 gagctcgggc aggacgtgcg ggtggtgcgc aacgacgaga tcagcgtcga ggagatcggc
     3421 gcgatgaagc ccgaccacct cgtgctgtcg cccggcccct gttcgcccgc cgaggccggc
     3481 gtgtgcgtcc cggcgatccg cgaatacctc ggcaagctgc ccatcctggg cgtgtgcctg
     3541 ggccaccaga gcatcggcgc cgcgctcggc ggccgcatca tccgcgcccg gcgccagatg
     3601 cacggcaagg ccagcacgct gaccaccgac ggcgaaggtg tctacaagga tctgccgcgc
     3661 gagttcagcg tgatccgcta ccactcgctg gtcatcgagc aggagacctg cccggccgaa
     3721 ctcatcgtca cgtcgcggtc ggaggacggc gagatcatgg gggtgcgcca ccgcgatttc
     3781 gtcggatcga ggacgccgat ggaaggcgtg cagttccacc ccgaatcgat cctgtcggag
     3841 cacgggcacg ccatgctgcg caacttcctc catctgagca ccctccaatg agaaacgcca
     3901 cgcgggcgca gcgccgggcc gccccaagca agcccgcggt ccccgcgggg gacggagcgc
     3961 cgtacccggc gcgggagggg cgccatgatt gacctgcgca gcgacaccgt cacccgcccc
     4021 acgccggcca tgcgcgacgc gatgttcgcg gcgccgctgg gcgacgacgt cttcggcgac
     4081 gaccccagcg tcaacgcgct gcaggagcgc atcgcggcgc tgctgggcaa ggaggcggcg
     4141 ctgttcatgc ccacgggcac gcagagcaac ctcgccgcgc tgatgagcca ctgccagcgc
     4201 ggtgacgaat acctcgtggg ccagatggcg cacacctacc gctgggaagg cggcggcgcc
     4261 gcggtgctgg gctcgatcca gccgcaaccc atcgcgcacc aggccgacgg cacgctcgcg
     4321 ctggccgaca tcgaggccgc catcaagccc gacgacgcgc acttcgcgcg cacccggctt
     4381 ctggcgctcg agaacacctt cggcggccag ccgatggcga tgtcgtacct gcgggacgcg
     4441 acggcgctcg cgcgcaggca cggcctggcc acgcacctgg acggcgcacg cgtcttcaac
     4501 gccgcggtcg tgcaggcgca ggccggcggc agcgatccgt acaccgaggt gcgcgccatc
     4561 gccgacctgt tcgacagcgt ctccgtgtgc ttcagcaagg gcctgggcgc gccggtcggc
     4621 tcggcgctgg tcggctcgcg cgagctgatc ggccgcgcca ggcgcgtgcg caagatggcc
     4681 ggcggcggca tgcgccaggc cgggctgctg gccgccgccg cgctgcatgc gctggaccac
     4741 cacgtcgagc gcctggccga ggaccacgcg ttggcgcgcc ggctggccga gggactggcc
     4801 ggcgtgccgg gcc
//
LOCUS       DATCRO010000220         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_542584, whole genome shotgun sequence.
ACCESSION   DATCRO010000220 DATCRO010000000
VERSION     DATCRO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542584"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VIN75_05985"
     CDS             <1..370
                     /locus_tag="VIN75_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8973748.1"
                     /translation="GMGDSIAAGVSIHASATGWLVLPADMPLVRPSSLRAVADALDQQ
                     PIAFAQHRGRRGHPVGFAAELFSELVMLKGDEGARRLLARYPTAAVELDDPGVLFDID
                     TVDDLAVAQRRIDGSSEIAI"
     gene            complement(664..1782)
                     /locus_tag="VIN75_05990"
     CDS             complement(664..1782)
                     /locus_tag="VIN75_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8973749.1"
                     /translation="MEERTKIHVGLDVHKDSITVAAAEPGRDKARLIGKIAHDAPRLM
                     KVLAKIGTVEELHVVYEAGPTGYGLQRALTKRGYVCEVIAPSKTPRQAGNRVKTDARD
                     SLELAEYSRAGKLSAVWIPDPADEAIRDLARAREDAVNSRRQVRQQLKGFLLRHDRRY
                     GGKTSWCGAHDHWLARQSFEASATQTAFTEYWQAVRSADERVERLTKALQGSIAGWRF
                     ESVVNALQALRGVAAITAIGLVAEIGDLNRFDHPRKLMGYLGLVPSEHSSGERTSRGS
                     ITKTGNGHARRLLVEAAWNYRFKARVGKEINARLEGLSEAVRTTAWKAQLRLTERFAA
                     LQKRGVQANKACVAVARELAGFVWAVGMQAHREKEVAA"
     gene            2352..>2801
                     /locus_tag="VIN75_05995"
     CDS             2352..>2801
                     /locus_tag="VIN75_05995"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8973750.1"
                     /translation="MSYTATPPLNATVAAVTERIRRRSAGTRADYLGHVDALRTRARG
                     AERLGCANVAHAFAGMPPNDKLRVVAERAPHIGVVTAYNDMLSAHQPYEGYPEQIRRD
                     AAALGATVQVAGGVPAMCDGVTQGAPGMELSLFSRDTIAMSTAVALSH"
BASE COUNT          414 a         1006 c          903 g          478 t
ORIGIN      
        1 gggtatgggc gattcgatcg ccgcgggcgt gtcgatccat gcgagcgcca ccggctggct
       61 cgtgctgccg gccgacatgc cgctcgtcag gccgagcagc ctgcgcgcgg tcgccgacgc
      121 gctcgaccag cagcccatcg cgttcgccca gcatcgcggg cgacgcggcc atcccgtggg
      181 cttcgcggcc gagctgttct ccgagctcgt gatgctcaag ggcgacgagg gcgcgcgccg
      241 cctgctcgcg cgctatccca ccgcggcggt cgagctcgac gaccccggcg tgctgttcga
      301 catcgacacc gtcgacgacc tcgccgttgc gcaacggcgc atcgacggct cgtccgagat
      361 cgcgatctag gggtctgcgc ggcagaagcc gcgcggactc ctcatatggt cgccgctgtc
      421 aatcgcgtta gcgcatgagt gctggcttgg cgtgggtctt ggatcgatcg ggtgatgcga
      481 ctcgagcgtt gcagcgacga tgaatcagac tcatatccgc ggattggcta ccagcaattc
      541 atcgatccgt cgtgggcctg cctcgctcta gggtcgggga tcagccttgc ggtgccctgg
      601 taggagtcac gagggttgcc ctatcgtgct cgtgccgcat cgcgcgatcg atccctacgg
      661 ctttcaggcg gcgacttcct tctcccggtg cgcttgcatg ccgacggccc agacgaaacc
      721 tgccagctcg cgggccacgg ccacacaggc cttgttcgcc tggacgccgc gcttttgcag
      781 ggccgcgaag cgctctgtca gacgcaattg cgccttccag gccgtcgttc gcaccgcctc
      841 ggacaggcct tccaggcgtg cattgatctc cttgcccacg cgggccttga accggtagtt
      901 ccaggctgct tcgaccagca gcctgcgtgc gtggccgtta cccgtcttgg tgatgctgcc
      961 tcggctcgtg cgttcaccac ttgaatgctc cgaaggcacc aggcccaggt agcccatgag
     1021 cttgcgtgga tggtcgaagc ggttgaggtc accgatctcc gccaccaggc cgatcgcggt
     1081 gatcgcggcc acgccgcgta atgcctgcaa ggcgttgacg accgactcga agcgccagcc
     1141 cgcgatcgag ccttgcagcg ccttggttaa gcgttcgacg cgttcgtccg ccgacctcac
     1201 cgcctgccag tactcggtga acgccgtctg cgtcgcggac gcctcgaagc tttgcctggc
     1261 cagccagtga tcgtgggcac cgcaccacga ggtcttgccg ccgtagcgcc tgtcgtgacg
     1321 aagcaggaag cccttgagct gttgacgcac ctgccggcgg ctgttgaccg cgtcctcgcg
     1381 cgcccgggcc aaatctcgga tcgcttcgtc ggccggatcg ggaatccaca ccgcgctcag
     1441 cttgcccgca cgcgaatact cggccagctc aagactatcg cgagcgtcgg tcttgacgcg
     1501 gttgcccgcc tgccgaggcg tcttcgacgg cgcgatgacc tcgcaaacat aacccctctt
     1561 cgtcagcgct cgttgcaagc cgtagcccgt gggcccggct tcgtagacga cgtgcagttc
     1621 ctcgacggtg ccgatcttgg ccagcacctt cataagcctt ggcgcatcgt gcgcaatctt
     1681 gccgatcagc cgcgccttgt ctcgcccggg ctcggccgcg gccaccgtga tgctgtcctt
     1741 gtggacgtcc aggcccacgt gaatcttggt acgctcttcc atggtcggtc tcctggttgc
     1801 tgacgacgca agtcacagca tgtagctcta cgcgcctcgg cgcgcaacct acgatgttgg
     1861 agaccggccg cccgaacggg ggcggcgacc atgatgtcta gcggatgaat gcacggctcc
     1921 cccgagttcc cctccgagag ggcgttccag catgttcgac cgcaccctcc agctccctcc
     1981 gagagggaga ctccagccag catgacgagg gcgctccacc tgctcagctg ccgctgagca
     2041 tggccatccg ccgcaagttc aacgccatgc acacgagcgt ccactccgcg cccatgcggt
     2101 gcaagcctcg catgctgatg gcagcatcgt gaaagcgcgc cgccgcagcg gcagcggcgg
     2161 cggccgatgg cggcgagcgg cgcatccgcg cgggcgacgc cggccccgcg ctcgccggcg
     2221 gggccgggaa ggcgcacact gcaaccggcg ggcgcaccgg ttgccgcccg gcgggcgcct
     2281 gaaatcgaca cgtcattccc tgctgcccgt gctggtttga ccatgtaacg gagttaccgg
     2341 ataatcaaac catgagttac accgccacgc cccccctgaa cgcgaccgtc gccgccgtca
     2401 ccgagcgcat ccgccgccgc agcgccggca cgcgcgccga ctacctcggc catgtcgacg
     2461 ccctgcgcac gcgcgcgcgc ggcgcggaac gcctgggctg cgccaatgtc gcccacgcgt
     2521 tcgccggcat gccgcccaac gacaagctgc gcgtggtggc cgagcgcgcg ccgcacatcg
     2581 gcgtcgtcac ggcctacaac gacatgctgt cggcgcacca gccgtacgag ggctatccgg
     2641 agcagatccg gcgcgatgcg gccgcgctcg gcgccacggt gcaggtggcc ggcggcgtgc
     2701 cggccatgtg cgacggcgtc acgcagggtg cgccgggcat ggagctgtcg ctgttctcgc
     2761 gcgacaccat cgcgatgtcc actgccgtcg cgctgtcgca c
//
LOCUS       DATCRO010000221         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_486219, whole genome shotgun sequence.
ACCESSION   DATCRO010000221 DATCRO010000000
VERSION     DATCRO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486219"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VIN75_06000"
     CDS             complement(<1..906)
                     /locus_tag="VIN75_06000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY8973751.1"
                     /translation="MKPLSALPALLGALVLPAAATGPMDPAAALQAEEANAVAVHDAI
                     APSVVKIDVSWQEPPPKLAPGLEQFLREDSQGKTTPRRGTGTGVLWDTSGHVVTSSTV
                     VEAAGGSFKVTLADGSRRDATLVAVDNRSKVAVLKLEGATTGLRPATPGSSGSLRVGQ
                     RIYVMGASFGKALVFVDGMLGAITSAYADDGYQDLAVNTSLNPGTAGGPLFDSAGRMV
                     GLVKGIYVGNTGGGFVLGTASDDIAWVVPRLLADGHVERAQIGISIQDLDALPLPPNL
                     PAGVAVFAVDAAGAGERAGLRPRDGD"
     gene            complement(1000..1791)
                     /locus_tag="VIN75_06005"
     CDS             complement(1000..1791)
                     /locus_tag="VIN75_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8973752.1"
                     /translation="MTSSFVLPALLGMLALPAMAQGPDAGAPSTSLTPQEVAAIAIHD
                     AIAPAVVQVDVRGTAAPRKLASFADVDKLFAGNPAASPPPTRHVGTGLAWDDAGHVVT
                     THAAVAVTDATITVTLADGSRRAARLIGDDPALDVAVLQVEGSPGTLHPLPLATGPGP
                     RPGHRVFAIGNPYGRGPLFIAGMVSAVDHTQEPGDPPRLLLAIVTSPGDAGGPVVDTS
                     GRLVGILRGQRGRPAPGVGIAAPVAELARVVPRLIAGGHVDAPAH"
     gene            complement(1868..2509)
                     /locus_tag="VIN75_06010"
     CDS             complement(1868..2509)
                     /locus_tag="VIN75_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8973753.1"
                     /translation="MNTASASSLWLYFVLVAGIIVLPGMDMAFVMASALAGGRRAGLA
                     AVGGLVAGGWLHLVMGAIGIGLLLQAAPGVFEALLVAGAVYIGWIGLSLLRGATALGE
                     AATAPRPALATFGRALATCLLNPKAYVFTLAVFPQFLHTGARSLPAQALSMAAITSFT
                     QVAVYGAIALAAAGTRAWLARNPGSQRRLGHAVGLLLIATAAWTAAHGLAAGR"
     gene            complement(2650..3126)
                     /locus_tag="VIN75_06015"
     CDS             complement(2650..3126)
                     /locus_tag="VIN75_06015"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8973754.1"
                     /translation="MTPALNKALPDFEALATNGVKFTPHAFQGRAVVLYFYPKDHTPG
                     CTTEAMQFRDMHGEFVKAGAVVFGVSRDNMASHEKFKQGLELPFELIADTEEKLCHMF
                     GVVKNKIMYGKKVKGIERSTFLIDGNGVLVAEWRGIKVAGHVEEVLKAVKDMKIKA"
     gene            complement(3348..3728)
                     /locus_tag="VIN75_06020"
     CDS             complement(3348..3728)
                     /locus_tag="VIN75_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY8973755.1"
                     /translation="MKFQPDTLAGVNNITHYDGDSLRVNGELRTKSVLVPWVGASLDW
                     DATRVEELTLAHFDAILALEPEVVIFGSGAKLRFVSPALYRNLIARRIGVETMDSGAA
                     SRTYTVLANEGRRAVAAILLAPAA"
BASE COUNT          579 a         1340 c         1500 g          550 t
ORIGIN      
        1 gtcgccgtcg cggggccgca ggccggcgcg ctcgcccgcg cctgcggcgt cgacagcaaa
       61 gaccgcgacg ccagcgggca ggttcggcgg cagggggagt gcgtccagat cctggatgct
      121 gatgccgatc tgcgcgcgct cgacgtgtcc gtcggccaga aggcgcggca ccacccacgc
      181 gatgtcgtcg gaggccgtgc cgagcacgaa gccgccgccg gtgttgccca cgtagatgcc
      241 cttgaccagg cccaccatgc gcccggcgct gtcgaacagc gggccgccgg cggttcccgg
      301 gttgagggag gtgttgaccg ccaggtcctg gtagccgtcg tccgcatagg cactggtgat
      361 cgccccgagc atgccgtcca cgaagaccag ggccttgccg aagctcgcgc ccatgacata
      421 gatccgctgg cccacgcgca gcgatccgct ggagcccggc gtggccgggc gcaggccggt
      481 cgtcgcgcct tcgagcttca ggaccgccac cttcgagcgg ttgtcgacgg caacgagcgt
      541 ggcgtcgcgg cggctgccgt cggcgagcgt gaccttgaac gacccgccgg cggcctcgac
      601 gacggtgctc gacgtcacca cgtgccccga ggtgtcccac agcacgccgg tgcccgtccc
      661 gcggcggggc gtggtcttgc cctgggagtc ctcgcgcagg aactgctcga ggccgggtgc
      721 gagcttcggg ggcggttcct gccacgacac gtcgatcttg accaccgacg gcgcgatggc
      781 gtcatggacg gccacggcgt tggcttcctc ggcctgcagc gccgcggccg gatccatcgg
      841 gcccgtcgcg gctgcgggca ggacgagcgc gcccaggaga gcgggcaggg cggacaaggg
      901 cttcatcggc gagcgatggc gggttggagg tggcgcaagc atacaggccc ggaacgcggc
      961 gtcgatgatg gcttgtcgcg agcctgcgga ttccttgctt cagtgcgccg gcgcgtccac
     1021 gtgaccgccg gcgatgagcc ggggcacgac gcgcgcgagt tcggcgaccg gcgccgcgat
     1081 gccgacgccg ggcgcagggc ggccgcgctg cccgcgcagg atgcccacga ggcggcccga
     1141 cgtgtccacc acggggccgc cggcgtcgcc ggggctggtc acgatggcca ggagcaggcg
     1201 gggcgggtcg cccggttcct gcgtgtgatc gacggcgctg accatccccg cgatgaagag
     1261 cggcccgcga ccgtaggggt tgccgatcgc gaagacgcga tggcccgggc gcggcccggg
     1321 accggtggcc aggggcaagg ggtgcagggt gccgggcgac ccctcgacct gcaggacggc
     1381 gacgtcgagt gccgggtcgt cgccgatcag gcgagccgcc cgccggctgc cgtcggccag
     1441 cgtgaccgtg atcgtggcgt cggtcacggc cacggccgcg tgggtcgtga cgacgtgccc
     1501 ggcgtcgtcc caggccaggc ccgtgccgac gtggcgcgtc ggcggcggcg acgccgccgg
     1561 attgcccgcg aagagcttgt cgacgtcggc gaacgatgcg agcttgcgcg gcgccgcggt
     1621 gccgcgcacg tcgacctgga cgacggccgg tgcgatggcg tcatggatcg cgatggccgc
     1681 cacttcctgc ggcgtgagcg acgtcgaggg cgcgccggcg tcgggcccct gggccattgc
     1741 cggcagtgcg agcatgccca gcagcgcggg caggacgaac gaagaggtca tggcacggaa
     1801 acggcgcaat ggcgatggcc gaaggataca ggccggtgcg ccgacgcccg ggtgatcggc
     1861 cggcggttca gcggcccgca gccaggccgt gcgccgcggt ccaggccgcc gtggcgatca
     1921 gcagcagccc gaccgcgtgg ccgaggcggc gctgtgaacc cggattgcgc gcgagccagg
     1981 cgcgggtgcc cgcggccgcg agcgcgatgg cgccatagac ggccacctgc gtgaacgacg
     2041 tgatggccgc catcgacagc gcctgggccg ggagcgatcg cgcgccggtg tgcaggaact
     2101 gcgggaagac ggccagcgtg aacacgtagg ccttcgggtt gagcaggcac gtggccagcg
     2161 cccggccgaa ggtggccagc gccgggcgcg gcgcggtcgc ggcctcgccg agcgcggtgg
     2221 cgccgcgcag cagcgacagg ccgatccagc cgatgtagac cgcgcccgcg accagcagcg
     2281 cctcgaacac ccccggcgcg gcctgcagca gcagcccgat gccgatcgcc cccatgacga
     2341 ggtgcagcca gccgcctgcg acgaggccgc cgacggccgc cagcccggcg cgccgcccgc
     2401 cggccaatgc gctggccatc acgaaggcca tgtccatgcc gggcagcacg atgatgccgg
     2461 cgacgaggac gaagtagagc cacagggagc ttgcggaggc ggtgttcatg gggcggatcg
     2521 tggaggtgaa ggatgacctc atgatccggg ccgatatgtg ccaccgcatg gcatgatttg
     2581 cggggcggat ggccgacccc atgaagaaag ccgcccggag gcggcttctg cggcggtgga
     2641 gcagggcgct caggccttga tcttcatgtc cttgacggcc ttgagcactt cctcgacgtg
     2701 gccggcgacc ttgatgccgc gccattcggc caccagcacg ccattgccgt cgatcaggaa
     2761 ggtcgagcgc tcgatgccct tgaccttctt gccgtacatg atcttgttct tgaccacgcc
     2821 gaacatgtgg cacagcttct cttcggtgtc ggcgatcagc tcgaagggca gctcgaggcc
     2881 ctgcttgaac ttctcgtgcg acgccatgtt gtcgcgcgaa acgccaaaaa cgaccgcgcc
     2941 ggccttgacg aattccccgt gcatgtcgcg gaactgcatg gcctcggtgg tacaacccgg
     3001 cgtgtggtcc ttggggtaga aatacagcac cacggcgcgg ccctggaagg catgcggggt
     3061 gaacttgacg ccattggtgg cgagtgcttc aaagtcaggg agcgctttgt tcagtgcagg
     3121 cgtcatgtag tgtctcggtc gatccgctgg tcgatctggg tgcttggcgt ggtggtggcc
     3181 ctcgcgactc gtcccgagat gacatcggtc caggtcaggc acaaccatcg attatagagt
     3241 gaactgccca tgtccccggg ccggacctcc atgggtttgc ccggctcgac aggcgcaggt
     3301 cgcatgagcg cgcgggatgc gcgctcgacg gcgcgccgga cggccgctca ggccgcgggc
     3361 gccagcagga tcgccgcgac ggcgcgccgg ccctcgttgg ccagcaccgt gtaggtgcgg
     3421 ctggcggcgc cgctgtccat cgtctcgacg ccgatgcggc gcgcgatcag gttgcggtag
     3481 agggcgggcg agacgaagcg cagcttcgcg ccgctgccga agatcaccac ctccggctcg
     3541 agcgccagga tcgcgtcgaa gtgcgccagc gtcagctcct cgacgcgcgt ggcatcccag
     3601 tcgagggatg cgcccaccca cggcaccagc acgctcttcg tgcgcagttc gccattgacg
     3661 cgcagggagt cgccgtcgta gtgggtgatg ttgttcactc cggcgagcgt gtcgggctgg
     3721 aatttcaagg ggctgtcctg ggctggaaag aggtcgctcg acggcacgcg gcgcgcaccg
     3781 ggaacgggtg tccgaattat aggaaaccgg gcctgcgcga gtcgccgcaa gggccaggcg
     3841 gcgccggggc gctgcgccca gccttcgccg ttgcgcaaga gatggttccg gccgcgcggc
     3901 gcgcggcgcc cgtcgaccgg cccggatgcc gcgcaaatgc gcggggatcg gtgcgccaca
     3961 gggtcgcgc
//
LOCUS       DATCRO010000222         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_835189, whole genome shotgun sequence.
ACCESSION   DATCRO010000222 DATCRO010000000
VERSION     DATCRO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835189"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VIN75_06025"
     CDS             <1..776
                     /locus_tag="VIN75_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8973756.1"
                     /translation="GLTLARTGAFGADLADVTFEVRRGEILGIAGVSGNGQQELMAAL
                     SGEDGRAPPDAIHLFGQPIGGHSPRRRRAQGLHFVPEERLGRGAVPSLSLAQNTLLTR
                     TEAVAASGWIRVGQATALARRLIERYNVKAAGPQAPARSLSGGNLQKFIVGREIDAGP
                     KLLIVSQPTWGVDVGAAAQIRGALIALRDAGCAVLVVSEDLDELFEISDRLVVIARGR
                     VSPSVPAPQATREQVGRWMSGLWNDEAANARGEAAHAAA"
     gene            763..1863
                     /locus_tag="VIN75_06030"
     CDS             763..1863
                     /locus_tag="VIN75_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8973757.1"
                     /translation="MLRLEARAAPSRAMSLLSPLLALAATVVIGIALFALLGKDPARG
                     LAMFFVEPFRGAYAVSELCVKATPLMLIGLGLAVCYRANVWNIGAEGQFVLGAIAATW
                     VALQATPDTGHWIVVPILLAGVAGGMAWAAIVAFLRDRANASEILVSLMLVYVAVQFL
                     NYLVYGTWKDPEGYNFPQTITFLDATKIPRLFTGLRVNIGFVAALLAVGAFWLYLFRA
                     FGGFRMQVGGLAAAAARYAGFSSRRALWTALLVSGGMSGLAGALEVAGPVGQLTPYVP
                     AGYGFAAIIVAFVGRLHPVGILFSSLLMSLFYIGGELAQSRLGLPKSLTGVFQGLLLF
                     SLLASDTLIHYRVRWRPAHPAHPTPAAKGPAA"
     gene            1863..>2258
                     /locus_tag="VIN75_06035"
     CDS             1863..>2258
                     /locus_tag="VIN75_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973758.1"
                     /translation="MDTYALLLAATLNAGTILAIAGLGLLINERAGVVNLGAEGMMLV
                     AAIAGFATAVQTGNDWLAFGAGALAGAAMAAAFGVLVIWLNTNQYATGLALSLFGSGF
                     SAFVGRLHPVGILFSSLLMSLFYIGGELAQ"
BASE COUNT          248 a          803 c          846 g          361 t
ORIGIN      
        1 gcgggctcac gctggcgcgc accggcgcgt tcggcgcgga cctcgcggac gtcaccttcg
       61 aggtgcgccg cggcgagatc ctgggcatcg cgggcgtctc gggcaacggc cagcaggagc
      121 tgatggcggc gctgtccggc gaggacggcc gcgcgccgcc cgacgcgatc catctgttcg
      181 gccagcccat cggcggccac tcgccgcgcc ggcgccgcgc gcagggcctg cacttcgtgc
      241 ccgaggagcg gctgggccgc ggcgcggtgc cgtcgctgtc gctcgcgcag aacacgctgc
      301 tgacgcgcac cgaggcggtg gcggcctcgg gctggatccg cgtcggccag gcgacggcgc
      361 tcgcgcggcg gctgatcgag cgctacaacg tcaaggcggc ggggccgcag gcgccggcgc
      421 gcagcctgtc gggcggcaac ctgcagaagt tcatcgtcgg ccgcgagatc gacgccggcc
      481 cgaagctgct gatcgtgtcg cagcccacct ggggggtgga cgtcggcgcc gcggcgcaga
      541 tccgcggcgc gctgatcgcg ctgcgcgacg ccggctgcgc ggtgctggtg gtcagcgagg
      601 acctggacga gttgttcgag atctccgacc gactggtcgt catcgcgcgc ggccgcgtct
      661 cgccgtcggt gcccgcgccg caggccacgc gcgagcaggt cggccgctgg atgagcgggc
      721 tgtggaacga cgaggccgcg aacgcgcgcg gggaggcggc gcatgctgcg gcttgaagcc
      781 cgcgcggcgc ccagccgggc gatgtcgctg ctgtcgccgc tgctggcgct cgcggccacg
      841 gtggtcatcg gcatcgcgct gttcgcgctg ctgggcaagg atccggcgcg cggcctggcg
      901 atgttcttcg tcgagccgtt ccgcggcgcg tacgcggtct ccgagctgtg cgtgaaggcc
      961 acgccgctga tgctgatcgg cctgggcctg gccgtctgct accgcgccaa cgtgtggaac
     1021 atcggcgccg agggccagtt cgtgctgggc gcgatcgcgg ccacgtgggt ggcgttgcag
     1081 gcgacgcccg acacgggcca ctggatcgtc gtgcccatcc tgctggcggg tgtcgcgggc
     1141 ggcatggcat gggcggcgat cgtggcgttc ctgcgcgacc gggccaacgc gagcgagatc
     1201 ctcgtgagcc tgatgctggt ctacgtggcg gtgcagttcc tcaactacct ggtctacggc
     1261 acctggaaag atccggaggg ctacaacttc ccgcagacca tcaccttcct ggacgcgacg
     1321 aagatcccgc gcctgttcac gggcctgcgc gtgaacatcg gcttcgtcgc ggcgctgctg
     1381 gccgtgggcg cgttctggct gtacctgttc cgcgcgttcg gcggcttccg gatgcaggtc
     1441 ggcggcctgg ccgcggcggc ggcgcgctac gccgggttct cgtcgcggcg cgcgctgtgg
     1501 acggcgctgc tggtctcggg cggcatgtcg ggcctggccg gcgcgctgga ggtggcgggt
     1561 ccggtcgggc agctcacgcc ctacgtgccc gcgggctacg gcttcgcggc catcatcgtc
     1621 gccttcgtcg gccgcctgca cccggtgggc atcctgttct cgtcgctgct gatgagcctg
     1681 ttctacatcg gcggcgagct cgcgcagtcg cgcctgggcc tgcccaagtc gctcaccggc
     1741 gtgttccagg gcctgctgct gttctcgctg ctggccagcg acacgctgat ccactaccgc
     1801 gtgcgctggc gtcccgcgca tcccgcgcat cccacgcccg ccgccaaggg gccggcagcc
     1861 tgatggacac ctacgcactc ctgctggccg ccacgctcaa cgccggcacc atcctggcca
     1921 tcgccggcct gggcttgctg atcaacgagc gcgcgggcgt cgtcaacctc ggcgccgagg
     1981 gcatgatgct cgtggccgcc atcgccggct tcgccaccgc ggtgcagacg ggcaacgact
     2041 ggctcgcgtt cggcgccggt gcgctcgcgg gcgcggcgat ggccgcggcg ttcggcgtgc
     2101 tggtgatctg gctcaacacc aaccagtacg ccacgggcct ggcgctgagc ctgttcggca
     2161 gcggcttctc ggccttcgtc ggccgcctgc acccggtggg catcctgttc tcgtcgctgc
     2221 tgatgagcct gttctacatc ggcggcgagc tcgcgcag
//
LOCUS       DATCRO010000223         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_823898, whole genome shotgun sequence.
ACCESSION   DATCRO010000223 DATCRO010000000
VERSION     DATCRO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823898"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VIN75_06040"
     CDS             complement(<1..971)
                     /locus_tag="VIN75_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY8973759.1"
                     /translation="MASTDRRIDLDWVRILAFGLLIFFHVGMYYVTWGWHVKSPFASH
                     AIEPLMVLSSPWRLSLLFFVSGCATAFLHSKSAAGFVRSRTQRLLIPLALGLWVIVPP
                     QSWAEVTEAVGYHGGYLHFMALYAQGYDGFCRDGCLRIPTWNHLWFVAYLWCYTMVAA
                     LAWLVVPQAAIDRCSAALARLLRGPGLIALPVAWLGAVRLLLVTRFPDTHALVDDWYL
                     HAEYFSVFALGVLLAREAGVWEEIRRQRWIALALAFAGYAAVQGGYAHWELMQSVDGL
                     RRLHRVAYAMDQWCAIVAALGFARQWNPADSRARRYLTEAIFPFYIV"
     gene            complement(1239..1985)
                     /locus_tag="VIN75_06045"
     CDS             complement(1239..1985)
                     /locus_tag="VIN75_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8973760.1"
                     /translation="MNLLLVDDEAPALAKLRRFLADDPGVRIAGEACDGDAALAFVAA
                     DPDAVDAVLLDIQMPGRSGLAVAAALPPHVLVAFATAYDAHAVQAFELNAVDYLLKPF
                     TRERLAACVGRLRDRLAPPERAQQRQRLATALHALQPVAGHWLVADRGELRRVPLAEI
                     ECVAAADNYIELHSATSTWLDRTTLAAFLAQPAAARFVRVHRSCAVQPACVAALEPLG
                     SGDAQLRLVSGRVIRLSRRYRDEWMRALGR"
     gene            complement(1982..3061)
                     /locus_tag="VIN75_06050"
     CDS             complement(1982..3061)
                     /locus_tag="VIN75_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8973761.1"
                     /translation="MTSVTRRILPWLAYAAFWAGIGLFFALAEWQHYVRSGGRHPWEP
                     FLWEMSGALTTALLAVAVYRWNDWLLAPGRGRAIAAAGYLGGFVPYTLAHSALMFGSR
                     YAVYFIAGLQYEPGSLRSVLGYESAKDAVSYALVLGLSLGWRAFVREQRQAAEVERLN
                     AHLAEARLARLQEQVQPHFLFNTFNLISSVMHEDVERADRILSELADLLRQSMSAAAV
                     TEHPLAQELRWVEPFLSIMRQRFGERLRTRIEVGDAAARCIVPALLLMAPVENAVKHG
                     VARSAAGVEVQVVADVVDGRLRIDIVDTGDAPLADHAGGTGLANLRARLATLHGDAAG
                     VTLAREGDRTRVRVTLPAARPGAAA"
     gene            3115..3741
                     /locus_tag="VIN75_06055"
     CDS             3115..3741
                     /locus_tag="VIN75_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8973762.1"
                     /translation="MNKADALGFVLAAVARLITGAQGHWKGVPPKAEQRIYFANHQSH
                     LDWVLIWATLPADLRVQTRPIAAADYWTKTPLKHWLTREVFRAVYVDRERKEGGPDPL
                     EPLVAALEQGDSLVIFPEGTRSNKGMPQDFKSGIFRLARQFPHVQLIPCWIDNVQRVM
                     PKGEVVPVPILCTVTFGAPCNLGPDEPKADFLARARDAVIALKPGGAA"
     gene            3738..4712
                     /locus_tag="VIN75_06060"
     CDS             3738..4712
                     /locus_tag="VIN75_06060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8973763.1"
                     /translation="MKLLQSLTATQQIGLLFLIVFGGLLLVSAGAVFLAMREKSPQDD
                     HGLTKFAADLRALWIGAVVFWLAWVSGAAGATILFAVFSFLALREFVTLMQTRRADHR
                     SLMLAFFVVLPVQYVLAGSRSFDLFTVFIPVYVFLAIPVLSALGGDPLRFLERNAKIQ
                     WGVMVCVFGLSHAPALLLLDFPQMHDRGPFLVFFLVIVTLTAQMLETFASTRLRRRPV
                     ARQIDRTFGWQSWALACLGAAFVGGLLFWITPFKLPQAMVMALIAGAAGTLGEFVMTA
                     LKRDAGVNYWGNTGQVTGAVGLLDRVAPLCFAAPVFFHSVRWYFQLKN"
     gene            4725..5273
                     /gene="ruvC"
                     /locus_tag="VIN75_06065"
     CDS             4725..5273
                     /gene="ruvC"
                     /locus_tag="VIN75_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8973764.1"
                     /translation="MRILGIDPGLQTTGFGVIEAEGATLRYVASGTISTAREAKGDLP
                     TRIKVIFEGVREVVQRYAPAQAAMEIVFVNVNPQSTLLLGQARGAALAALVSSGLPVS
                     EYTATQMKKGVVGHGQAKKEQIQEMVARLLALPGLPGKDAADALGLAITHAHAGTTMA
                     ALAAGATLARRQHAQYRGGRTY"
BASE COUNT          828 a         1972 c         1967 g          734 t
ORIGIN      
        1 tgcacgatgt agaacgggaa gatcgcctcg gtcaggtagc gccgcgcgcg cgagtcagcc
       61 gggttccact ggcgggcgaa gccgagcgcg gcgacgatcg cgcaccactg gtccatcgcg
      121 taggcgacgc gatgcagccg gcgcaagccg tccaccgact gcatcagctc ccagtgcgcg
      181 tagccgcctt gcaccgccgc atagcccgcg aacgcgagcg ctagcgcgat ccagcgctgg
      241 cggcggatct cctcccagac gccggcctcg cgcgcgagca gcacgccgag cgcgaacacg
      301 ctgaagtact cggcatgcag gtaccagtcg tcgacgagcg cgtgggtgtc gggaaagcgc
      361 gtgaccagca gcaggcggac cgcgccgagc caggccaccg gcaacgcgat caggcccggg
      421 ccgcgcagca ggcgcgcgag cgcggcgctg cagcgatcga tggccgcctg cggcacgacg
      481 agccacgcga gcgccgccac catcgtgtag caccacaggt aggcgacgaa ccacaggtgg
      541 ttccaggtcg ggatgcgcag gcagccgtcg cggcagaagc cgtcataccc ctgggcgtac
      601 agcgccatga agtgcaggta gccgccgtgg tagccgacgg cctcggtcac ctcggcccag
      661 gactgcggcg gcacgatgac ccacagtccc agggccagcg ggatcagcag ccgctgggtg
      721 cgcgagcgca cgaagccggc ggccgacttc gaatgcagga aggcggtcgc gcagcccgag
      781 acgaagaaca gcaggctcag ccgccacggc gagctgagca ccatcagcgg ctcgatcgcg
      841 tgggacgcga acggcgactt cacatgccag ccccaggtca cgtagtacat gcccacgtgg
      901 aagaagatca gcaggccgaa ggccaggatg cggacccagt cgaggtcgat gcggcggtcg
      961 gtggaggcca tggcgcgcgg cttcggagtg atggagaccg catcgtcgcg atgcgccgcc
     1021 gctcgcgcga gcgatttggg gcgagtcgca tttgccctgg ggcgagtggc gacgctggcg
     1081 gagccggggc ttgactggca ggggtggatc ctgcgactgc gcgcaggatg acggctgcct
     1141 ttcccttgtc ttgcgcgcct ggcgacggct tcccattgcc tgtcatcctg cgcggagcgg
     1201 agcgcggtcg caggatccac cccggcgagg acgcccggtc agcgccccag cgcccgcatc
     1261 cactcgtccc gatagcgccg cgacagccgg atcacccgcc ccgacaccag ccgcagctgc
     1321 gcatcgccgc tgcccagcgg ctcgagcgcc gcgacgcagg ccggctgcac ggcgcacgag
     1381 cggtgcacgc gcacgaagcg tgccgcggcc ggctgcgcga ggaaggccgc gagcgtcgtg
     1441 cgatcgagcc aggtggaagt ggccgaatgc agctcgatgt agttgtcggc cgcggccacg
     1501 cactcgatct cggccagcgg cacgcggcgc agttcgccgc ggtcggcgac cagccagtgc
     1561 ccggccaccg gctgcagcgc gtgcagcgcg gtggccaggc gctggcgctg ctgcgcgcgc
     1621 tcgggcggcg cgaggcgatc gcgcagtcgc ccgacgcacg cggccaggcg ctcgcgcgtg
     1681 aacggcttga gcaggtagtc caccgcgttg agctcgaacg cctgcacggc gtgcgcgtcg
     1741 taggccgtgg cgaacgccac cagcacgtgg ggcggcagcg cggcggccac cgccagtccg
     1801 ctgcggccgg gcatctggat gtccagcagg acggcgtcga ccgcgtcggg atcggccgcg
     1861 acgaaggcca gcgcggcatc gccgtcgcag gcctcgccgg cgatccgcac gccgggatcg
     1921 tcggccagga agcggcgcag cttggccagc gccggcgcct cgtcgtcgac cagcagcagg
     1981 ttcacgcggc ggcccccggg cgcgcggcgg gcagcgtgac gcgcacgcgc gtgcggtcgc
     2041 cctcgcgcgc gagcgtcacg ccggctgcgt cgccgtgcag cgtggccagg cgcgcgcgca
     2101 ggttcgcgag gccggtgccg ccggcatggt cggccagcgg ggcgtcgccg gtgtcgacga
     2161 tgtcgatgcg caggcggccg tcgacgacgt cggcgacgac ctgcacctcg acgcccgcgg
     2221 cgctgcgcgc cacgccgtgc ttgacggcgt tctccaccgg cgccatcagc agcagcgcgg
     2281 gcacgatgca gcgcgcggcg gcgtcgccga cctcgatgcg cgtgcgcagc cgctcgccga
     2341 agcgctggcg catgatggac aggaagggct cgacccagcg cagctcctgg gccagcggat
     2401 gctccgtgac ggcggcggcg ctcatcgact ggcgcagcag gtcggccagc tcggacagga
     2461 tgcggtcggc gcgctcgacg tcctcgtgca tcaccgacga gatcaggttg aacgtgttga
     2521 acaggaagtg cggctgcacc tgctcctgca gccgcgcgag ccgcgcctcg gccaggtgcg
     2581 cgttcagccg ctcgacctcc gcggcctggc gctgctcgcg cacgaaggcg cgccagccca
     2641 gcgacaggcc cagcaccagc gcgtacgaca cggcgtcctt ggcgctctcg tagccgagga
     2701 ccgatctcag gctcccgggc tcgtactgca ggccggcgat gaaatagacg gcgtagcgcg
     2761 atccgaacat cagcgcagag tgcgccagcg tgtagggcac gaagccgccg aggtagcccg
     2821 cagcggcgat cgcgcggccg cgcccgggcg ccagcagcca gtcgttccag cggtagacgg
     2881 ccaccgccag cagggccgtc gtcaacgcgc ccgacatctc ccacaggaac ggctcccacg
     2941 gatgccgccc gccggagcgc acgtagtgct gccattcggc cagcgcgaag aacaggccga
     3001 tgcctgccca gaaggccgcg taggccagcc agggcaggat gcggcgcgtg acggaagtca
     3061 tgcctgcatt gtgacgacgg attcggcccg cgcgggccca ctacactccc ggccatgaac
     3121 aaagccgacg cattgggctt cgtgctggcg gcggtcgcga gactgatcac cggcgcgcaa
     3181 ggccactgga agggcgtccc gccgaaggcg gaacagcgca tctacttcgc caaccaccag
     3241 agccatctcg actgggtgct gatctgggcc acgctgcccg ccgacctgcg cgtgcagacg
     3301 cgccccatcg ccgccgccga ctactggacg aagacaccgc tcaagcactg gctcacgcgc
     3361 gaggtgttcc gcgcggtcta cgtcgaccgc gagcgcaagg agggcggacc cgacccgctg
     3421 gagccgctcg tggccgcgct cgagcagggc gactcgctcg tcatcttccc ggagggcacg
     3481 cgcagcaaca aaggcatgcc gcaggacttc aagagcggca tcttccggct cgccaggcaa
     3541 ttcccgcacg tgcagctgat cccgtgctgg atcgacaacg tgcagcgcgt catgcccaag
     3601 ggcgaggtcg tgccggtgcc catcctgtgc accgtcactt tcggcgcgcc gtgcaacctc
     3661 ggccccgatg aacccaaggc cgacttcctc gcgcgggcgc gcgacgcggt catcgcgctc
     3721 aagccggggg gcgcggcatg aagctgctgc aatcgctcac cgccacgcag cagatcgggc
     3781 tgctgttcct gatcgtgttc ggcgggctgc tgctcgtgtc ggccggcgca gtgttcctcg
     3841 cgatgcgcga gaagtccccg caggacgacc acggactgac gaagttcgcc gccgacctgc
     3901 gcgccctgtg gatcggcgcg gtcgtgttct ggctcgcgtg ggtctcgggc gcggccggcg
     3961 cgacgatcct gttcgcggtg ttctcgttcc tcgcgctgcg cgagttcgtc acgctgatgc
     4021 agacgcggcg tgccgaccat cgcagcctca tgctcgcgtt cttcgtggtg ctgccggtcc
     4081 agtacgtgct ggcgggcagc cgcagcttcg acctgttcac cgtgttcatc ccggtctacg
     4141 tgttcctcgc gatcccggtg ctcagcgcgc tcggcggcga cccgctgcgc ttcctggagc
     4201 gcaacgcgaa gatccagtgg ggcgtgatgg tgtgcgtgtt cggcctgtcg cacgcgccgg
     4261 cgctgctgct gctggacttc ccgcagatgc acgaccgcgg gccgttcctc gtgttcttcc
     4321 tggtcatcgt gacgctgacc gcgcagatgc tggagacctt cgccagcacg cgcctgcgcc
     4381 ggcgtcccgt ggcgcgccag atcgaccgca cgttcggctg gcagtcctgg gcgctcgctt
     4441 gcctgggcgc cgccttcgtc ggcggcctgc tgttctggat cacgccgttc aagctgccgc
     4501 aggcgatggt gatggcgctc atcgccggcg ctgccggcac gctcggcgaa ttcgtgatga
     4561 cggcgctcaa gcgcgacgcc ggcgtcaact actggggcaa caccggccag gtcaccggcg
     4621 cggtgggcct gctcgaccgc gtcgcgccgc tgtgcttcgc ggcgccggtg ttcttccatt
     4681 cggtgcgctg gtacttccag ctcaagaact gaaccccggt catgatccgc atcctcggca
     4741 tcgaccccgg cctgcagacc accggcttcg gcgtgatcga ggccgaaggc gccacgctgc
     4801 gctacgtggc cagcggcacg atcagcacgg ccagggaggc caagggcgac ctgccgacgc
     4861 gcatcaaggt gatcttcgag ggcgtgcgcg aggtcgtgca gcgctacgcg ccggcgcagg
     4921 ccgcgatgga gatcgtgttc gtcaacgtca acccgcagag cacgctgctg ctgggccagg
     4981 cgcgcggggc ggcgctggcg gcgctggtgt cgtcggggct gccggtgtcg gagtacaccg
     5041 ccacgcagat gaagaagggc gtggtcgggc acgggcaggc gaagaaggag cagatccagg
     5101 agatggttgc ccgcctgctg gccctgcccg gcctgcccgg caaggatgcc gccgacgcgc
     5161 tgggcctggc gatcacccac gcccacgccg gcacgacgat ggccgcgctg gccgccggcg
     5221 ccacgctcgc gcgccgccag cacgcccagt accgcggcgg tcggacatac tgacgcccac
     5281 cgcgccgccc cgcgcgggac gacaatgcag cctgggcgtc gatgcgcgcg ccgacgctgc
     5341 cctccatcgc ttcctcacga cacctcatgg ccgacgcaac tccctacttc ggaatcgccc
     5401 ctttcgtgct cggcatgagc cgcgagctcg cgcgcgcggc cgcgggcaag cccgactccg
     5461 tcgagacgac gaacgacgac gagggcgccg cggtcgagac c
//
LOCUS       DATCRO010000224         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_936305, whole genome shotgun sequence.
ACCESSION   DATCRO010000224 DATCRO010000000
VERSION     DATCRO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936305"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VIN75_06070"
     CDS             complement(<1..1657)
                     /locus_tag="VIN75_06070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8973765.1"
                     /translation="MSDVPKQLGKYQIRRAIGKGAMGIVYEGFDPIIDRIVAIKTIKK
                     DELDAEEAEEHSRRFLIEAKAAGRLNHKNIVGIYDYGEEKGLSFIVMEFVQGKELKSF
                     FAAKHPFSMSQIVRLMGELLDALGYSHSRGVIHRDVKPDNIFVTDDGSVKLGDFGIAR
                     IDSTSKTHAGTVLGTPSYMAPEQIRGETADARSDLYAAGVILYQFLVGERPFSGGMVA
                     VMQKVLMEAPKPPSSHNPSIPEAIDMVVLKALAKIPSERFTSAAEFSRELTAAVGLAD
                     SEDESESTMIMSADALRALQEGLPPSERTGVGSTHTGTGSTHTGTGTGTGGTGPAGRS
                     AGGRGAAIEEARRRAEEARRRLEEEARRAEIELQQAEAEQRAAEAEAQAQFEQSMASS
                     QALLADAKALHDAGQALFVQNTLLVDGPGHATAVRAAAGRLRANAALIGEFISQSYAL
                     APASQSELETRQSEMTAAADAQDALAERVEREGKAALTSFEQKLEVLEKRLSSHDGAW
                     GNESRALEALDPAAGEMSIVPIQGRLDKLAASVEQVAKGLATVQ"
     gene            complement(1778..>4442)
                     /locus_tag="VIN75_06075"
     CDS             complement(1778..>4442)
                     /locus_tag="VIN75_06075"
                     /inference="COORDINATES: protein motif:HMM:NF039490.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973766.1"
                     /translation="LSASGSSVSLGATTVGGNLTAAASTGAIGQTGVVAVTGTSTLGA
                     SAGAVTLANPANDFGGAVTASGSTVSLAGANALTAHVDSSGNATVSAGGALSVDGTVG
                     GSLGATSTGPITQSAALTVGGPSVLDAGTAAITLASAGNDFGGTVTATGSTVSLADAN
                     ALAVHVTSSAGTTLGAGGALAIEGTIGGGLTATSNGPMTQSAALTVGGTSTLNAGTGA
                     ITLANAGNDFTGAVSATGGAVSLTDANALVVHLGGASSATLNAGGALAVDGSTSGALT
                     ANGNGVVLGATTVGGNLAANSGTGTITQSGALAVTGASTLDAGGHAITLANAANDFGG
                     AVTASGGAVSLSDANALTAHLTATSATLVAGTQLTADGATTGTVTVTAASADVEQAGA
                     LTVSLNNVGSAIVKSGAALHVDGNSTGDVTASGSTVTLGGSTLAVGGNLAATSTAGDI
                     TQAGTLSVTGTSTLAAGSGNVTLADSANALGGAVTASGANVTLADASALTAHLAANGS
                     ATLTSASGLSVDGSAGALDVTGTSVAFGAAGTTVNGALTTNGGGVTQSGTLTVTGLSA
                     FNAGTNDILLASPGTNLQGAITATGHTISLTDAHAIDANVNATGDVTLNSTGGDATAK
                     GSFGGALTISGAKVTLDQGTLATPMVIKGKLDVTSSGDTSLTYVTADGAQVSAGGALM
                     LNGTLKSTGGDVTLKANTIQGVNKLGAIDATAGSALLLDTTGGGSIGKVTPTLGVLDA
                     DAIISLLGATGQGGMNVQFGTGSSAWFRVASQQQTALLHPQSSSSSQTFFCDMATCVN
                     VLGQTTSIADSVIANILSTASQDAADAAFGTENLDFAIRKGYVTTIGRVPPGIDEIAG
                     DLGATACDSRVTSPTAIAAEKACSAGAKAAK"
BASE COUNT          522 a         1559 c         1640 g          721 t
ORIGIN      
        1 cctgcacggt ggccaagccc ttggcgacct gttcgacgct ggccgccagc ttgtccaggc
       61 ggccctggat cgggacgatg gacatctcgc ccgccgccgg gtcgagcgcc tccagcgcac
      121 gcgactcgtt gccccaggcg ccgtcgtggc tggacaggcg cttttccagc acctccagct
      181 tctgctcgaa gctggtcagc gccgccttgc cttcgcgctc gacgcgctcg gccagcgcgt
      241 cctgcgcgtc ggccgcggcg gtcatctcgg actggcgcgt ctccagttcg gactgcgacg
      301 cgggcgccag cgcataggac tggctgatga actcaccgat cagcgccgcg ttcgcgcgca
      361 gcctgccggc agccgcacgg acggcggtgg cgtggccggg gccgtccacc aacagcgtgt
      421 tctgcacgaa cagcgcctgg ccggcgtcgt gcagcgcctt ggcatcggcc agcagggcct
      481 gcgagctcgc catcgactgc tcgaactggg cctgcgcctc ggcttcggcg gcgcgttgtt
      541 cggcctcggc ctgctgcagt tcgatttcgg cgcgcctggc ctcttcctcg aggcggcgcc
      601 gcgcctcctc ggcgcgccgg cgggcttcct cgatggccgc gccgcggccg ccggccgagc
      661 gccctgccgg cccggtgccg cccgtgcccg tgcccgtgcc cgtgtgcgtg ctgccggtgc
      721 cggtgtgcgt gctgccgacg ccggtgcgct cggacggcgg caagccctcc tgcagcgccc
      781 tgagcgcgtc ggccgacatg atcatcgtcg actcggactc gtcctcggag tcggccagcc
      841 caacggccgc cgtcagctcg cggctgaatt cggcggcgct ggtgaagcgc tcggaaggga
      901 tcttggccag cgccttcagg acgaccatgt cgatcgcttc ggggatggac gggttgtggc
      961 tggacggcgg cttgggcgcc tccatcagca ccttctgcat gacggccacc atgccgccag
     1021 agaacggccg ctcgccgacc aggaactggt acaggatgac gccggccgcg tacaggtcgg
     1081 agcgcgcgtc ggccgtctcg ccgcggatct gctcgggcgc catgtagctg ggcgtgccca
     1141 gcaccgtgcc ggcgtgcgtc ttggaggtgg aatcgatgcg ggcgatgccg aagtcgccca
     1201 gcttcaccga accgtcgtcg gtgacgaaga tgttgtcggg cttgacgtcg cggtggatga
     1261 ccccgcggct gtgcgagtag ccgagcgcat cgagcagttc gcccatcagc cgcacgatct
     1321 gcgacatcga gaacgggtgc ttggcggcga agaaggactt gagctccttg ccctgcacga
     1381 actccatgac gatgaaggac aggcccttct cctcgccata gtcgtagatg ccgacgatgt
     1441 tcttgtggtt gaggcggccg gcggccttgg cctcgatcag gaagcgccgc gagtgctcct
     1501 cggcctcctc ggcgtcgagc tcgtccttct tgatcgtctt gatggcgacg atccggtcga
     1561 tgatcggatc gaatccctca tagacgatgc ccatcgcacc cttgccgatc gctcggcgga
     1621 tctggtactt gcccagttgt ttgggtacgt cgctcaaagg gcttgtggat caacgacgat
     1681 gcccggcgag ccgggcatcg gagcggccgc cgcgtgggac ggcgcccgcc tgttcaagtg
     1741 gtgacgatcc ggacggggcg gcgccgcccc gccagaatca cttggccgcc ttggcgcctg
     1801 cggaacaggc cttctcggcg gcgatggccg tcggcgatgt tacccgagag tcacacgccg
     1861 tcgccccgag gtcacccgcg atctcgtcga tgccgggcgg gacgcggccg atggtggtca
     1921 cgtagccctt gcggatcgcg aagtcgaggt tttcggtgcc gaacgccgcg tcggcggcat
     1981 cctgcgaggc cgtgctcagg atgttggcga tcacggagtc ggcgatggac gtcgtctggc
     2041 ccagcacgtt gacgcaggtg gccatgtcgc agaagaaggt ctgcgacgaa ctggacgact
     2101 gcgggtgcag cagcgcggtc tgctgctgcg aggccacgcg gaaccacgcg ctgctgcccg
     2161 tgccgaactg gacgttcatg ccgccctggc cggtggcgcc caggagcgag atgatcgcgt
     2221 cggcatcgag cacgccgagc gtcggcgtga ccttgccgat gctgccgccg ccggtggtgt
     2281 ccagcaagag cgccgaacct gccgtcgcat cgatcgcgcc cagcttgttg acgccctgga
     2341 tcgtgttcgc cttgagcgtg acgtcgccgc cggtggactt cagggtgccg ttcagcatca
     2401 gcgcgccgcc cgccgacacc tgcgcgccgt cggccgtcac gtaggtcagc gaggtgtcgc
     2461 ccgaggaagt gacgtccagc ttgcccttga tgaccatcgg ggtcgcgagc gtgccctggt
     2521 cgagcgtgac cttggcgccg ctgatcgtca gcgcgccgcc gaagctgccc ttggcggtgg
     2581 cgtcgccgcc ggtggagttc aaggtcacgt cgccggtggc gttgacgttg gcgtcgatgg
     2641 cgtgcgcgtc ggtcaggctg atggtgtggc cggtggccgt gatcgcgccc tgcaggttgg
     2701 tgcccggcga ggccagcagg atgtcgttcg tgccggcatt gaaggccgac aggcccgtga
     2761 ccgtgagcgt gcccgactgg gtgacgccgc cgccgttggt ggtcagcgcg ccgttgaccg
     2821 tcgtgcccgc ggcgccgaag gcgacgctgg tgccggtgac gtcgagcgcg ccggcgctgc
     2881 cgtccacgga caggccgctg gccgaggtca gcgtggcgct gccgttggcc gccaggtgtg
     2941 ccgtcagcgc cgacgcatcc gccagcgtga cattcgcgcc cgaggccgtc accgcgccgc
     3001 ccagggcgtt ggcgctgtcg gcgagcgtga cgttgcccga gccggccgcc agcgtgctgg
     3061 tgccggtgac cgacagcgtg cccgcctgcg tgatgtcgcc ggccgtcgag gtggccgcca
     3121 ggttgccgcc gaccgcgagc gtgctgccgc ccagggtgac cgtcgaaccg ctggcggtga
     3181 cgtcgcccgt gctgttgccg tcgacgtgca gcgccgcgcc ggacttgacg atggcgctgc
     3241 cgacgttgtt caggctcacc gtcagcgcgc ccgcctgttc gacgtccgcc gacgcggccg
     3301 tgaccgtcac ggtgccggtg gtggcgccgt cggcggtcag ttgcgtgccc gcgacgagcg
     3361 tcgcgctggt ggccgtcagg tgcgccgtca gcgcgttcgc gtcggacagc gacaccgcgc
     3421 cgccgctcgc ggtgaccgcg ccgccgaagt cgttggccgc gttggccagc gtgatggcgt
     3481 ggccgccggc gtcgagcgtg gacgcgcccg tcaccgccag cgcgcccgac tgcgtgatcg
     3541 tgccggtgcc gctgttggcc gccaggttgc cgcccaccgt cgtggccccg aggaccacgc
     3601 cgttgccgtt ggccgtgagc gcgccgctgg tgctgccgtc gaccgccagc gcgccgcccg
     3661 cgttcagggt ggccgagctc gcgccgccca ggtgcacgac gagcgcgttg gcgtcggtca
     3721 ggctgaccgc gccgccggtg gccgacacgg cgccggtgaa gtcgttgccg gcgttggcca
     3781 gcgtgatggc gccggtgccg gcattgagcg tgctggtgcc gccgacggtc agcgcggccg
     3841 actgcgtcat cgggccgttg ctggtggcag tgaggccgcc gccgatcgtg ccctcgatcg
     3901 ccagcgcgcc gcccgcgccg agcgtggtgc cggcgctgct ggtgacgtgc acggcgagcg
     3961 cgttcgcgtc ggccagcgag acagtgctgc cggtggccgt gacggtgccg ccgaagtcgt
     4021 tgccggcgct ggccagcgtg atggccgcgg tgcccgcatc gagcacgctg ggaccgccca
     4081 ccgtcagcgc agccgactgc gtgatcggcc cggtgctggt ggcgcccagg ctgccgccga
     4141 cggtgccgtc gaccgacagc gcgccgcccg cggacacggt ggcgttgccg ctgctgtcga
     4201 cgtgggccgt cagcgcgttc gccccggcca ggctcacggt gctgccgctg gccgtgaccg
     4261 cgccaccgaa atcgttggcc ggattggcca gcgtgacggc gcccgcgctg gcgccgagcg
     4321 tgctcgtgcc cgtcaccgcg acgacgccgg tctggccgat ggcgcccgtc gaggccgcgg
     4381 ccgtcaggtt gccgccgacc gtggtggcgc ccagcgagac gctgctgccg ctggccgaca
     4441 gg
//
LOCUS       DATCRO010000225         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_666338, whole genome shotgun sequence.
ACCESSION   DATCRO010000225 DATCRO010000000
VERSION     DATCRO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666338"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIN75_06080"
     CDS             <1..375
                     /locus_tag="VIN75_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973767.1"
                     /translation="ASFTYLRQLPFDTIKIDGMFVRDMDTDLVHGGMVRSMTEMARLL
                     KKPVVAEFVETEAVAVLLRELGVDWAQGFHYHVPEPLTADALRASLAPAAAGSAAVAA
                     TAPASAAPAPGAESATPPAALA"
     gene            complement(425..1603)
                     /locus_tag="VIN75_06085"
     CDS             complement(425..1603)
                     /locus_tag="VIN75_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973768.1"
                     /translation="MKTLSIANGELWLHAPGAGPRQIESPFARELIERDLQSRRNTGW
                     KNAPREQQTGVIPSSSLWGRRGPGGDNAVLVPRFEQAVRGDDDDALYYLLSVGETRGL
                     FRRHLSEDREVRLFHRSGWTCNGFAYNPGDKRLILAAQHANGSAHLEVYDDEGTRRGA
                     ITDGDCVDASPSLVPGRSRTVVYQSSGVARHPQVGHVMAVGHACVHMLDYGSGQLEVL
                     LDDAKFDHVAPRMDAQGALYAIRRPALKPQHERAGVALKDTLLMPFRLLKAVFGYLNF
                     FSMVYGKEPLRSAGGPRTPELDQDLGQLWLHGRMIELSKARNDPQYAGSLVPANWELV
                     RVARAGAPVEVLARHVAGFDVRDDGRIVYTNGYDILELEGSEKRTLARQEQVANLSAL
                     "
     gene            complement(1647..2555)
                     /locus_tag="VIN75_06090"
     CDS             complement(1647..2555)
                     /locus_tag="VIN75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY8973769.1"
                     /translation="MPMTLDGRLVVAISSRALFDFEEENRVFEGNDDRAYMQLQLERL
                     DVPARPGVAFSLVRKLLQFRDPAPRVEVVILSRNDPVSGMRAFRSAKHYGLPIERGTF
                     TRGRSPWRYLRPLGAHLFLSANDGDVRSALEAGVPAARVVPHAARASDAHPHEVRIAF
                     DGDAVLFSDEAERVFQAEGLDAFQAHESSNWARPLAPGPFKPLLEALQLLQRSSTDAM
                     KVRTALVTARSAPAHERAIRTLMQWQIDVDEAMFLGGLPKGEFLREFEPDFFFDDQTR
                     HVENAAPHVPSGHVAAGISNLPPGAA"
     gene            2704..3351
                     /locus_tag="VIN75_06095"
     CDS             2704..3351
                     /locus_tag="VIN75_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973770.1"
                     /translation="MTTAQATATPASAGTAPFTAPPSPREPSGRAWVGRFPTSASVAD
                     CAQPFRANLTAFIAAMGAAGASVNVAATLRPPQRAYLMHWCWSIVKQKTDPRSIPPLD
                     GVAIAWAHLDGQGGYDPIASLAAAQEMVEAYGMQALNVAPALQSNHISGTAVDMAISW
                     SGTLSIARRDGVVVAIDSQPRTGMNPDLKAVGATYGVLKFVGGASDMPHWSADGH"
BASE COUNT          464 a         1228 c         1218 g          464 t
ORIGIN      
        1 gcgtccttca cctacctgcg ccagctgccg ttcgacacga tcaagatcga cggcatgttc
       61 gtgcgcgaca tggacaccga cctcgtgcac ggcggcatgg tgcgctcgat gaccgagatg
      121 gcgcgcctgc tgaagaagcc ggtggtggcc gagttcgtcg agaccgaggc cgtcgccgtg
      181 ctgctgcgcg agctgggggt cgactgggca cagggcttcc actaccacgt gcccgagccg
      241 ttgacggccg atgcgctgcg cgcgagcctc gcgccggcgg cggcgggcag cgccgcggtc
      301 gccgcgaccg cccccgcgag cgcggccccc gcaccgggcg ccgaatcggc aacgccgccc
      361 gcggcactgg cgtaacccac ccaccgccgg agcggtccgg cgtcgcccga gtcgcctcgg
      421 cggcctacag ggcgctgagg ttggccacct gctcctggcg cgccagcgtg cgcttctcgc
      481 tgccttcgag ctcgaggatg tcgtagccgt tcgtatagac gatgcggccg tcgtcgcgca
      541 cgtcaaagcc ggccacgtgc ctggcgagca cctccaccgg cgcgccggcg cgcgccacgc
      601 gcaccagttc ccagttggcc ggcaccaggc tgcccgcgta ctgcgggtcg ttgcgcgcct
      661 tggacagttc gatcatgcgg ccatgcagcc acagctggcc caggtcctgg tcgagctcgg
      721 gcgtgcgcgg cccgccggcc gagcgcaggg gctccttgcc gtagaccatg ctgaagaagt
      781 tcaggtagcc gaacaccgcc ttgagcagcc ggaacggcat cagcagcgtg tccttgagcg
      841 ccacgccggc gcgctcgtgc tgcggcttca gcgcgggacg gcggatggcg tacagcgcgc
      901 cctgggcgtc catgcgcggg gcgacgtggt cgaacttcgc gtcgtccagc agcacctcca
      961 gctggccgct gccgtagtcc agcatgtgca cgcaggcgtg gcccacggcc atgacgtggc
     1021 cgacctgcgg atggcgcgcg acgccgctcg actggtagac gaccgtgcgc gagcgccccg
     1081 gcaccaggga cggcgacgcg tcgacgcagt cgccgtcggt gatggcgccg cggcgcgtgc
     1141 cctcgtcgtc gtagacctcc aggtgcgcgc tgccgttggc gtgctgcgcc gcgaggatca
     1201 ggcgcttgtc gccggggttg tacgcgaagc cgttgcaggt ccagccgctg cggtggaaca
     1261 gccgcacctc gcgatcctcg gacagatggc ggcggaacag gccgcgcgtc tcgccgacgg
     1321 acagcaggta gtacagcgcg tcgtcgtcgt cgccgcgcac cgcctgctcg aagcgcggca
     1381 ccagcaccgc gttgtcgccg cccgggccgc gccggcccca caggctggag ctggggatga
     1441 cgccggtctg ctgctcgcgc ggcgcgttct tccagcctgt gttgcgccgg ctctgcaggt
     1501 cgcgctcgat cagctcgcgc gcgaacggcg actcgatttg gcgcgggccg gcgcccggcg
     1561 cgtgcaacca gagttcgccg ttggcgatcg acagcgtctt catcggacag gccctccctt
     1621 tgacgcgatt atccgcccgc ggacgttcac gcggcgcccg gcggcaggtt ggagatgccc
     1681 gcagccacgt ggcccgaggg cacgtgcggc gctgcgttct cgacgtggcg ggtctggtcg
     1741 tcgaagaaga agtcgggctc gaactcgcgc aggaactcgc ccttgggcag gccgccgagg
     1801 aacatcgcct cgtcgacatc gatctgccac tgcatcagcg tgcggatcgc gcgctcgtgc
     1861 gcgggggcgc tgcgcgccgt caccagcgcg gtgcgcacct tcatcgcgtc ggtggacgaa
     1921 cgctgcagca gctgcagcgc ctccagcagc ggcttgaacg ggccgggcgc gagcgggcgc
     1981 gcccagttgg aggattcgtg ggcctggaac gcgtcgaggc cctcggcctg gaacacgcgc
     2041 tcggcctcgt cggagaacag aacggcgtcg ccatcgaagg cgatgcggac ctcgtgcgga
     2101 tgcgcgtccg acgcgcgcgc cgcgtgcggc acgacgcgcg cggccggcac gcccgcctcg
     2161 agcgccgagc gcacgtcccc gtcgttggcc gacaggaaca ggtgcgcgcc gagcgggcgc
     2221 aggtagcgcc acggactgcg gccgcgcgtg aaggtgccgc gctcgatggg caggccgtag
     2281 tgcttggccg agcggaacgc gcgcatgccc gagaccgggt cgttgcgcga caggatgacg
     2341 acctcgacgc gcggcgccgg gtcgcggaac tgcagcagct tgcgcaccag cgagaacgcg
     2401 acgcccgggc gcgccggcac atccaggcgc tcgagctgca gctgcatgta ggcgcggtcg
     2461 tcgttgccct cgaagacgcg gttctcctcc tcgaagtcga acagcgcgcg ggacgagatg
     2521 gcgacgacga gtcgcccgtc gagagtcatg ggcatggggg aatcataggc gccccaccgg
     2581 ctcgcccggt gagccggtgc cagggttttc gggccatccg cgcatccctt ctttgaacct
     2641 gccggcgtcg cgcacccggg cgtaagctgg cgccgcctcc gaccgccttg cagggagaac
     2701 gccatgacca ctgcccaggc caccgccacg ccggccagcg ccggcaccgc gcccttcacc
     2761 gccccgccgt cgccgcgcga gcccagcggc cgcgcctggg tcgggcgctt tcccaccagc
     2821 gcctcggtgg ccgactgcgc gcagccgttc cgcgccaacc tcaccgcgtt catcgccgcg
     2881 atgggcgcgg ctggcgccag cgtcaacgtc gcggccacgc tgcggccgcc gcagcgcgcc
     2941 tacctgatgc actggtgctg gtccatcgtc aagcagaaga ccgacccacg ctcgatcccg
     3001 ccgctcgacg gcgtggcgat cgcctgggcg cacctggacg gccagggcgg atacgacccc
     3061 atcgcgtcgc tggccgcggc acaggagatg gtcgaggcct acggcatgca ggcgctcaac
     3121 gtggcgccgg cgctgcagtc caaccacatc tcgggcacgg cggtggacat ggccatcagc
     3181 tggtcgggca ccttgtcgat cgcgcggcgc gacggcgtcg tggtcgccat cgacagccag
     3241 ccgcgcaccg gcatgaaccc cgacctgaag gcggtgggcg ccacgtacgg cgtgctcaag
     3301 ttcgtgggcg gcgcgagcga catgccgcac tggtcggccg atggccactg aagcgcgcgc
     3361 gttcgtcgcg gccg
//
LOCUS       DATCRO010000226         5187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_396268, whole genome shotgun sequence.
ACCESSION   DATCRO010000226 DATCRO010000000
VERSION     DATCRO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396268"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VIN75_06100"
     CDS             complement(<1..254)
                     /locus_tag="VIN75_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8973771.1"
                     /translation="MTSHWLFQLVVLAVLLAWVVGAYNRMVRLRTAIVSAWEQIVAAL
                     VKRSEAMAAVVEAVREPLVSEAATLQALAEADAKQRTAADG"
     gene            complement(251..967)
                     /gene="queC"
                     /locus_tag="VIN75_06105"
     CDS             complement(251..967)
                     /gene="queC"
                     /locus_tag="VIN75_06105"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY8973772.1"
                     /translation="MIDPRSALVLFSGGQDSTACLAWALARHARVETIGFDYGQRHRI
                     ELECRAGVREGLAALDPDWAARLGDDHLLDLSLLGQVSDTALTSDRAIEFQANGLPNT
                     FVPGRNLLFLGFAATVAYRRGASVLVGGMCETDFSGYPDCRDNTLKAMQVALSLGLAA
                     PMTIETPLMFLTKAQTWALTRSLGGDALNELIVDKTHTCYLGERGARHAWGHGCGTCP
                     ACQLRAAGHAGFLASLADDA"
     gene            1138..2013
                     /locus_tag="VIN75_06110"
     CDS             1138..2013
                     /locus_tag="VIN75_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973773.1"
                     /translation="MHPARQASRPAASLAALGLLAALVSCGGGVVPDPGGTSPDVRLN
                     CAGPCVPSDTLSTGAITAYLYVVDDGTRTQAQAGFNTDGSIFHNVELEGDTLWFVDGD
                     RQTRMALSREGAWDFLLDLLTLGQPYLADIAPQPTTARDYQFQLRRAGGTVTSQVTLP
                     APFEFATPGMDQVFPIGAAAVPITVTRALDVGAFSTTFVCTDANGNQTSNRVPDGRSF
                     GQVDASGANFQFDVRAFLASLEWDQDSFPPAAVARCDVTLRAIDSAAGALAADFHAFS
                     ILQATQLRSVRILLR"
     gene            complement(2078..3448)
                     /locus_tag="VIN75_06115"
     CDS             complement(2078..3448)
                     /locus_tag="VIN75_06115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY8973774.1"
                     /translation="MQAPSRRSHTRRETKSRAPWRTWRLADNPGMSESDVAATLDALG
                     AAARAAASRMAASPRARRDAALRALAARLREAVAELESANAQDLDAAAANGVTGPLLD
                     RLRLDAHALDTVIAGCLQLAEMPDPIGEITGVAQRPSGIRVGRMRVPLGVFGMIYESR
                     PNVTIEAASLAIKSGNACILRGGSEAIRSNVALWRCVQRALADAGLPEAAVQLVETTD
                     RAAVGRLVAMPEFVDVIIPRGGRGLIERVAREARVPVIKHLDGNCHTYVDERVDLELA
                     VRVTDNAKTQKYSPCNATESLVVHAAQAGAFLPRIGAVFAAKGVEMRGDARALAALKD
                     VPGAKLVPARDSDWGEEYLAPIVSIRVVDSLDEAIAHINTHGSHHTDAILTTDHPNAM
                     RFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDKFHARGPVGLEGLTSQKWIVLG
                     EGEVRG"
     gene            3574..4188
                     /locus_tag="VIN75_06120"
     CDS             3574..4188
                     /locus_tag="VIN75_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973775.1"
                     /translation="MKPIIAQLALAAAALVPTLAHAGLDSDNPFHPLVGIALTGGGDK
                     LSSVEYTNGDSVDITAGGLVQFYGGLEYHEKGSPFGFQGTFGYHVDSAGARNGNQRFS
                     RWPIEGIALFNVAPQFRLGAGLRYAASAKFTSDGAGYVGNADFNSHLGGILMGEWLIT
                     PSMGLQLRYVNEKYKIKGVAADGVTPVDYTIDGSHGGIGFNYYF"
     gene            4414..5061
                     /locus_tag="VIN75_06125"
     CDS             4414..5061
                     /locus_tag="VIN75_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8973776.1"
                     /translation="MTRLTFPTALRRPAPALALAAAFACAAPALAAPSPAEGTTPEAA
                     APARIRPVAGVLVTGSAGKTPGFFTNPDGTTVTGSLGGRYEAFAGAEFPVDPNGLALR
                     VTAGIHVTSGMTASNGASEHFSRFPLEATFWYPLNDKLRIGAGGRYSLRPRFSGPGGR
                     TSDKLSSMPALVMGVGYQLIPHVLLEGRYVYERFEQYGGDDVEASHWGLGVTAIY"
BASE COUNT          704 a         1945 c         1833 g          705 t
ORIGIN      
        1 ccgtcggccg ccgtgcgttg cttcgcgtcg gcctcggcca gcgcctgcag cgtcgcggcc
       61 tcggagacca gcggctcgcg caccgcctcc accaccgcgg ccatcgcctc gctgcgcttg
      121 accagcgccg cgacgatctg ctcccaggca gagacgatgg ccgtgcgcag gcgcaccatg
      181 cggttgtacg cgcccaccac ccaggcgagc agcaccgcca gcaccacgag ctggaacagc
      241 cagtgcgagg tcatgcgtcg tcggccaggc tcgccaggaa gccggcgtgg cccgcggcgc
      301 gcagctggca ggccgggcac gtcccgcagc catggcccca ggcgtggcgc gcgccgcgct
      361 cgcccaggta gcaggtgtgg gtcttgtcga cgatgagctc gttgagcgcg tcgccgccca
      421 gcgagcgcgt cagcgcccag gtctgcgcct tggtgaggaa catcagcggc gtctcgatcg
      481 tcatcggcgc cgccagcccc aggctgaggg cgacctgcat cgccttgagc gtgttgtcgc
      541 ggcagtcggg atagcccgag aagtcggtct cgcacatgcc gcccaccagc accgacgcgc
      601 cgcgccggta ggccacggtg gccgcgaagc cgaggaacag caggttgcgc ccggggacga
      661 acgtgttggg caggccattg gcctggaact cgatcgcgcg gtcggacgtc agcgcggtgt
      721 cggagacctg gcccagcagc gacaggtcga gcaggtggtc gtcgcccagg cgcgcggccc
      781 agtccgggtc cagcgcggcc aggccctcgc gcacgccggc gcggcactcg agctcgatgc
      841 ggtggcgctg gccgtagtca aagccgatcg tctccacgcg cgcgtgacgc gcgagcgccc
      901 aggcgaggca ggcggtggag tcctggccac cggaaaagag caccagggcg ctgcggggat
      961 cgatcatgga aaggggcgtg ggtgcgggtc gtgcgggcca ttgtcgccgg attggcgagg
     1021 cgccgccgcg gatcgggaac gtgcccgccg cgccctccag gcaaggggtc gcgccggacg
     1081 cgggggcgtc ccagaatgcg gaccgcgcgc ggcatcgggc cgcccgagga gttcgcgatg
     1141 caccctgctc gacaggccag tcgcccggcc gcgagcctcg ccgccctggg cctgctggcc
     1201 gcgctggtgt cctgcggcgg cggcgtcgtg cccgacccgg gcggcacctc gcctgacgtg
     1261 cgcctgaact gcgccggtcc gtgcgtgccg tccgacaccc tctccaccgg cgcgatcacc
     1321 gcctacctgt acgtggtcga cgacggcacc cgcacccagg cgcaggccgg cttcaacacc
     1381 gacggctcga tcttccacaa cgtcgagctc gagggcgaca cgctgtggtt cgtcgacggg
     1441 gaccgccaga cccgcatggc cctgtcgcgc gaaggcgcgt gggacttcct gctggacctg
     1501 ctcacgctgg ggcagcccta tctggccgac atcgcgccgc agccgaccac cgcgcgcgac
     1561 tatcagttcc agctgcgccg cgccggcggc accgtcacca gccaggtgac gctgcccgcg
     1621 ccgttcgagt tcgcgacgcc gggcatggac caggtcttcc ccattggcgc cgccgccgtg
     1681 ccgatcacgg tcacgcgtgc gctggacgtg ggcgccttca gcaccacctt cgtgtgcacc
     1741 gatgccaacg gcaatcagac gagcaaccgc gtgcccgacg gccggtcgtt cgggcaggtg
     1801 gacgcgagcg gcgcgaactt ccagttcgac gtgcgggcct tcctggcgag cctggagtgg
     1861 gaccaggaca gcttcccgcc cgccgcggtc gcgcgctgcg acgtgacgct gcgcgcgatc
     1921 gacagcgccg ccggcgcgct cgcagccgac ttccacgcct tctcgatcct gcaggcgacg
     1981 cagctgcgca gcgtgcgcat cctgctgcgg taagacgcct tgaacgaatc gcgcacgccc
     2041 gatcggcgcg ccggatccat tcacggcttc atggcgctca gccgcgcact tcgccctcgc
     2101 ccagcacgat ccacttctgc gaggtcaggc cctccaggcc gaccgggccg cgggcgtgga
     2161 acttgtcggt ggagatgccg atctccgcgc cgagtccgta ctcgaacccg tcggcgaagc
     2221 gcgtgctggc gttgaccatg acgctggccg aatcgacctc gcgcaggaag cgcatcgcgt
     2281 tcgggtggtc ggtggtgagg atggcgtcgg tgtggtgcga gccgtgggtg ttgatgtggg
     2341 cgatggcctc gtccagcgaa tcgacgacgc ggatgctgac gatgggcgcc aggtactcct
     2401 cgccccagtc gctgtcgcgc gcgggcacga gcttcgcgcc aggcacgtcc ttgagcgccg
     2461 cgagggcgcg cgcgtcgccg cgcatctcga cgcccttggc cgcgaacacg gcgccgatgc
     2521 gcggcaggaa cgccccggcc tgcgcggcgt gaaccaccag cgactcggtg gcgttgcacg
     2581 ggctgtactt ctgggtcttg gcgttgtcgg tgacgcgcac ggccagctcg aggtcgacgc
     2641 gttcgtcgac gtaggtgtgg cagttgccgt ccaggtgctt gatgaccggc acgcgggcct
     2701 cgcgcgccac gcgctcgatc aggccccggc cgccgcgcgg gatgatgacg tcgacgaact
     2761 cgggcatcgc gacgaggcga cccacggcgg cgcggtcggt cgtctcgacc agctgcaccg
     2821 cggcctcggg caggccggcg tcggcgagcg cgcgctgcac gcagcgccac agcgcgacgt
     2881 tggaccggat cgcctccgag ccgccccgca ggatgcacgc gttgccgctc ttgatggcca
     2941 gcgacgccgc ctcgatcgtg acgttggggc ggctctcgta gatcatgccg aacacgccca
     3001 gcggcacgcg catgcggccc acgcggatgc cgctggggcg ctgcgcgacg ccggtgatct
     3061 cgccgatcgg gtcgggcatc tcggccagct gcaggcagcc ggcgatgacg gtgtcgagcg
     3121 cgtgcgcatc caggcgcagg cgatcgagca gcgggccggt gacgccgttg gcggcggccg
     3181 cgtccaggtc ctgggcgttg gccgactcca gctccgcgac ggcctcgcgc aggcgcgccg
     3241 caagcgcgcg cagcgcggcg tcgcgcctgg cgcgcggcga cgcggccatg cgcgaggcgg
     3301 cggcgcgggc ggcggcgccg agggcatcga gggtggcggc gacatcggat tcggacatgc
     3361 ccggattgtc cgccagccgc caagtcctcc acggagcgcg agatttcgtc tctcggcgag
     3421 tgtgggatcg ccgcgacggt gcctgcaggg tcgcaacggt cgctcgctaa cattcgcggg
     3481 ccgcgaccgc tcgcaccacg cgagccgggc aggcggcacg gcgcacggga ggcctcggcg
     3541 ccgcttgtca accaagcatc cgaaagagat cacatgaagc ccatcatcgc ccagctggcc
     3601 ctcgcggccg ccgcgctcgt ccccacgctc gcccacgccg gcctggacag cgacaacccg
     3661 ttccacccgc tcgtcggcat cgccctcacc ggcggcggcg acaagctgtc gtccgtcgaa
     3721 tacaccaacg gcgactccgt cgacatcacc gccggcggcc tcgtccagtt ctacggcggc
     3781 ctcgagtacc acgagaaggg ctcgccgttc ggcttccagg gcaccttcgg ctaccacgtc
     3841 gacagcgccg gcgcccgcaa cggcaaccag cgcttctcgc gctggccgat cgaaggcatc
     3901 gcgctgttca acgtggcccc gcagttccgc ctgggcgccg gcctgcgcta cgccgcgtcg
     3961 gccaagttca ccagcgacgg cgccggctac gtcggcaacg cggacttcaa ctcgcacctg
     4021 ggcggcatcc tgatgggcga gtggctgatc accccgtcga tgggcctgca gctgcgctac
     4081 gtcaacgaga agtacaagat caagggcgtc gccgccgacg gcgtcacgcc ggtcgactac
     4141 acgatcgacg gcagccacgg cggcatcggc ttcaactact acttctgacg ccgcgcggcg
     4201 gcgcgcgccg gcgcttgccc ggagcccggt aagatcgcag gcgccgccgg cccgacgtcc
     4261 cgccgcgctg cccgcgccgc cgcccgcccg cccgccgggt gtggcccgcc ggcgcgccag
     4321 ccggcttccg tcgcgacctt tccggcttgt cggcgcctcg cgcgcccggg tgggcgcacg
     4381 cacgcgcgcc ccgaccagcc aggaaccgtc ccgatgacac gcctcacttt cccgaccgcg
     4441 ctccgccgcc cggcccctgc cctcgcgctg gcggccgcct tcgcctgcgc cgccccggcg
     4501 ctggccgcgc cctcgcccgc cgagggcacc acccccgagg cggcggcgcc ggcccgcatc
     4561 cgcccggtgg ccggtgtgct ggtcaccggc agcgccggca agactcccgg cttcttcacg
     4621 aatccggacg gcacgaccgt caccggcagc ctgggcggcc gctacgaggc cttcgccggc
     4681 gcggagttcc ccgtcgaccc gaacggcctc gcgctgcgcg tgacggccgg catccacgtg
     4741 acgtccggca tgaccgccag caacggcgcc agcgagcact tctcgcgctt cccgctggag
     4801 gccacgttct ggtacccgct caacgacaag ctgcgcatcg gcgcgggcgg gcgctactcg
     4861 ctgcgtccgc gcttcagcgg ccccggcggc cgcacgtccg acaagctcag ctccatgccg
     4921 gccctggtga tgggcgtggg ctaccagctg atcccgcacg tgctgctgga gggacgctac
     4981 gtctacgagc gcttcgagca gtacggcggc gacgacgtcg aggccagcca ctgggggctg
     5041 ggcgtcacgg cgatctattg agggaagtgg cgtcgtcgcg gatcaggccg catcgccgtg
     5101 gagttgacgc aacgccgtca tccgcgatga cgcggcgtgt gctccgtgcg acgcactgtc
     5161 gccgcgttcg cccaccaaat gtcatcc
//
LOCUS       DATCRO010000227         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_373613, whole genome shotgun sequence.
ACCESSION   DATCRO010000227 DATCRO010000000
VERSION     DATCRO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373613"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VIN75_06130"
     CDS             <1..755
                     /locus_tag="VIN75_06130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8973777.1"
                     /translation="QLTLSITRPLKQAVLFASAVGSGQLDAPLPRTSDDECGMLLLAL
                     KAMARQLQEANARLQRLAIEDGLTGAFNRRHFDDVLFAEHERARRAAMRRNSEGVQDA
                     AAHLALLLIDVDHFKAFNDRFGHPAGDACLRAVVGAVRDAGLRPGDFLARYGGEEFVV
                     ILPSCDIAGAAVVAERVRRQVESLHARPDTTLPAPVSVSIGLAAMCDARDSRPADLLR
                     AADLALYDAKHRGRNQVRERALECCTTAAAAA"
     gene            complement(758..1372)
                     /gene="ribA"
                     /locus_tag="VIN75_06135"
     CDS             complement(758..1372)
                     /gene="ribA"
                     /locus_tag="VIN75_06135"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY8973778.1"
                     /translation="MAFSLERASCRLNTAYGDFQMHGFKDLASGLEHVALCLGDIASP
                     PPVLTRMHSECLTGDAFGSCHCDCGPQLRGALQAIAKEGRGIVLYLRQEGRGIGLLNK
                     IRAYALQEQGADTLDANRLLGLPADARDYGIAAAMLSELGATSIRLMTNNPTKIDSLQ
                     AHGITIAERVPLQVGRNRRNGAYLDTKAHRMGHLFDVLREPSDE"
     gene            1528..1998
                     /locus_tag="VIN75_06140"
     CDS             1528..1998
                     /locus_tag="VIN75_06140"
                     /inference="COORDINATES: protein motif:HMM:NF042776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6714 family protein"
                     /protein_id="HEY8973779.1"
                     /translation="MPYTDLIAEIFAAFSSEGTAFPPMTLRGGDALDADQPAPPFDVL
                     VDAVSDEYLEAHPWGSGYLDAASWRHYLPFMMEYALRHIDRPSDVTDALLTSFRPPDR
                     DPPRLGSLDKAQETVVLRFLDALAHGPGSASSDLAAMVLSEWWTPGAIPKDLED"
BASE COUNT          274 a          752 c          779 g          327 t
ORIGIN      
        1 ggcagctgac cctgagcatc acgcggccgc tgaagcaggc cgtgctgttc gcgtcggcgg
       61 tcggcagcgg gcagctggat gcgccgctgc cgcgcacttc cgacgacgag tgcggcatgc
      121 tgctgctggc gctgaaggcg atggcccggc aactgcagga ggccaacgcg cgcttgcagc
      181 gcctggccat cgaggacggc ctgaccggcg cgttcaaccg ccgccacttc gacgacgtgc
      241 tgttcgccga gcatgagcgc gcgcgccgcg cggcgatgcg ccgcaacagc gaaggcgtgc
      301 aggatgcggc cgcgcacctg gcgctgctgc tgatcgacgt cgaccacttc aaggccttca
      361 acgaccggtt cggccacccg gcgggcgatg cctgcctgcg cgccgtcgtg ggcgcggtgc
      421 gcgacgccgg cctgcgcccg ggcgacttcc tggcgcgcta cggcggcgag gagttcgtcg
      481 tgatcctgcc gtcctgcgac atcgccggcg ccgccgtggt ggccgagcgt gtgcggcggc
      541 aggtggagtc gctgcacgcg cggcccgaca cgacgctgcc ggcgccggtg tcggtcagca
      601 tcggcctggc cgccatgtgc gacgcgcgcg attcccggcc ggccgacctg ctgcgcgcgg
      661 ccgacctcgc gctgtacgat gccaagcacc gcggccgcaa ccaggtgcgc gagcgcgcgc
      721 tggagtgctg cacgacggcc gccgcggcgg cctgatccta ttcgtcgctg ggctcgcgca
      781 gcacgtcgaa caggtggccc attcgatggg ccttggtgtc gaggtaggcg ccgttcctgc
      841 ggttgcgccc gacctgcagc ggcacgcgct cggcgatcgt gatgccgtgc gcctgcaggc
      901 tgtcgatctt ggtcgggttg ttggtcatca ggcggatcga cgtcgcgccc agttcggaca
      961 gcatcgccgc ggcgatgccg tagtcgcgcg cgtcggccgg caggcccagc aggcggttgg
     1021 cgtccagcgt gtcggccccc tgttcctgca gcgcgtaggc gcggatcttg ttcagcaggc
     1081 cgatgccgcg gccttcctgg cgcaggtaca gcacgatgcc gcggccctcc ttggcgatcg
     1141 cctgcagcgc gccgcgcaac tgcgggccgc agtcgcaatg gcagctgccg aacgcgtcgc
     1201 cggtcaggca ttcggagtgc atgcgggtga gcacgggcgg cggcgaggcg atgtcgccga
     1261 ggcacagcgc gacgtgctcg agtccggacg caagatcctt gaatccgtgc atctggaagt
     1321 cgccgtaggc ggtgttgagc cggcaggaag cgcgttcgag ggagaaggcc atgcgtgggt
     1381 gtcgttgaag cccgatttta agtcggcggc aatgtccttg cggcgcgcgg tcgagcgagc
     1441 tctccaactc ggtgactcgg ggcgcggtga ccgcgccggc gaggttgtgc gacagtaccg
     1501 tcatcctccc gcgcgcccga gccgcccatg ccctacaccg acctcatcgc cgagatcttc
     1561 gcggcgttct ccagcgaagg caccgccttc ccgccgatga cgctgcgcgg tggcgacgcg
     1621 ctcgatgccg accagcccgc cccgccgttc gacgtgctgg tggacgccgt ctccgacgag
     1681 tacctcgagg cccacccctg gggcagcggc tacctggacg ccgcctcctg gcgccactac
     1741 ctgccgttca tgatggagta cgcgctgcgc cacatcgacc ggcccagcga cgtcaccgat
     1801 gccctgctga cctcgttccg gccgcccgac cgcgatccgc cgcgcctggg ctcgctggac
     1861 aaggcgcagg agacggtggt gctgcgcttc ctggatgcgc tcgcccacgg ccccggctcc
     1921 gccagcagcg acctggcagc gatggtgctg tcggagtggt ggacgccggg ggcgattccg
     1981 aaggatctgg aggactgagc gggatcgcgc gcagggggca ggcctgtcct gcgtccgcga
     2041 tcggctcggg ctcctgcgtt cggcctggcg ccgcggacga aggagaggcc agatccctcc
     2101 ggcggcttca gggcgctgcc gccatcttcg cc
//
LOCUS       DATCRO010000228         7061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1026152, whole genome shotgun sequence.
ACCESSION   DATCRO010000228 DATCRO010000000
VERSION     DATCRO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026152"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VIN75_06145"
     CDS             <1..280
                     /locus_tag="VIN75_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8973780.1"
                     /translation="TKATLGLKVVDLSDAERARLHVKSGVRVDSVEGAAARAGVQEGD
                     VILQIGNVPVSSAKQFVATVAKLDKSKSIAVLVRRGDSANYLVIRPSK"
     gene            431..2095
                     /gene="ettA"
                     /locus_tag="VIN75_06150"
     CDS             431..2095
                     /gene="ettA"
                     /locus_tag="VIN75_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY8973781.1"
                     /translation="MAQYVFTMNRLGKIVPPKREILKGVSLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDKEFEGEATPMPGLNIGYLPQEPQLNPDQTVRQSVEEGMGGVLAAKK
                     RLEEVYIEYGNEDADFDKLAAEQAELEAIISSAGSDNVEHQLEIAADALRLPAWDAVV
                     GNLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRFGGTVVAITHD
                     RYFLDNAAEWILELDRGRGIPYKGNYSEWLLQKEQRLEQEQKTEDARTKAMKKELEWV
                     RQNPKGRQAKSKARMARFEELSDFDYQKRNETNEIFIPVADRLGNEVIEFTNVSKGFG
                     DRQLIDNLSFKVPAGAIVGIIGPNGAGKSTLFRMIAGKEQPDSGTVSIGKTVKMAFVD
                     QSRSELQDAKTVWEDVSGGLDNLTVGKFVMPSRAYIGRFNFKGNDQQKLVGQLSGGER
                     GRLHLAKTLIQGGNVLMLDEPSNDLDVETLRALEDALLEFAGSIMVISHDRWFLDRIA
                     THILACEGDSQWVFFDGNYQEYEADKKKRLGEEGARPKRVRYKALK"
     gene            2250..2855
                     /locus_tag="VIN75_06155"
     CDS             2250..2855
                     /locus_tag="VIN75_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8973782.1"
                     /translation="MTINALKCAAAAALLATAGVAGAATVTFDSLTNPGIWAYTGDTY
                     TEQGMSFAATVRSNGYTLYAYGLESPFNADPDGATLHQEFEGDYGIFVSPAGAGGTFT
                     LTSFDLANSWNDASGGNVDFRYVDATGAHHETLTPADQIGLQTFTFDLTGVRSFSLWS
                     NNYQLDNVQVNDIIPVPEPSSYAFLLAGLAGIIGLSRRRKN"
     gene            complement(2952..3716)
                     /locus_tag="VIN75_06160"
     CDS             complement(2952..3716)
                     /locus_tag="VIN75_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY8973783.1"
                     /translation="MQIRSLARLVALCAATVPLLALAQVTQKPDGQWRSLFTAGASIA
                     TGNTDAKSASLGADIIKLTDSDKWAVNGAAQYARSNGATTANHVGGSTIYASDFTPQW
                     FGFGELDLLHDVPSDLSLRTTLGSGIGYHVVKKPDQSFDLSAGLAYTMDRFRHPQVIE
                     DETRSRYDHAELLLAEESNNKLSDTTTFKQKLTVYPNLRHTGDVRTVFDAGLSVAMTK
                     QLALTATLSHRYDSRPGQGLGRNDTLFVTGVSLRFD"
     gene            complement(3841..4290)
                     /locus_tag="VIN75_06165"
     CDS             complement(3841..4290)
                     /locus_tag="VIN75_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973784.1"
                     /translation="MSRVDAARRALLRVAAATAVAGVAGCAAPGQPKEKRMSDDDMSF
                     SRIGPPDVDPVEFEGRRYEQIINGQDEHLDQRTGYLAVTDVATGKRLAAVKVYAVAFD
                     RDIEADVQDVFFVHLELQAAERRLLVENEHGQRFYVALDGYAVTAAP"
     gene            complement(4299..5360)
                     /locus_tag="VIN75_06170"
     CDS             complement(4299..5360)
                     /locus_tag="VIN75_06170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEY8973785.1"
                     /translation="MPSTGFADEYRLCRETLEGQDFGGALPGLLDLAKALGAEGPELN
                     FYGKVSHFREVALKFKEADKIMEAAGVPATGLVKDDAVKMAASLKMLRHLYLAGARGS
                     QQVWVVSTPSSYAKFASDELLDVKADATKVKAKLADVTEQFSEETKDHLAAAMQLGLS
                     WCESAKVVLGNAASTPASRAIVERWFAASDTAATDVDKTIAALLAGFKKIAFALNHNQ
                     VVITDMPMYRSDAKKNTVEAFVRRTDGKFERPRTIYIEKALFENYDISVLHDMKKNWA
                     RVLVHECTHTEVGTQDNGYAFRGIGPGAKIAADKAATNADSWAFFAADCAGALTDGER
                     LRALGGTGGKLVKQAKNWN"
     gene            complement(5553..5723)
                     /gene="rpmG"
                     /locus_tag="VIN75_06175"
     CDS             complement(5553..5723)
                     /gene="rpmG"
                     /locus_tag="VIN75_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8973786.1"
                     /translation="MAAKGGREKIKLESTAGTGHFYTTNKNKKTTPEKLEFMKFDPKA
                     RKHVLYKEIKLR"
     gene            complement(5745..5978)
                     /gene="rpmB"
                     /locus_tag="VIN75_06180"
     CDS             complement(5745..5978)
                     /gene="rpmB"
                     /locus_tag="VIN75_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY8973787.1"
                     /translation="MARVCQVTGKHPMTGNNVSHANNRTKRRFLPNLQNRRIWVESEN
                     RFVRLRITTAALRLIDKNGIDAVLADLRAKGEL"
     gene            complement(6146..>7061)
                     /gene="trxB"
                     /locus_tag="VIN75_06185"
     CDS             complement(6146..>7061)
                     /gene="trxB"
                     /locus_tag="VIN75_06185"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8973788.1"
                     /translation="SGPAGYTAALYAARANLKPMLVTGLAQGGQLMTTTEVDNWPADV
                     AGVQGPDLMARFLAHAERFDTRIVFDHVNKVDLSQRPFRLTGDSGTYTCDALIIATGA
                     SAKYLGLPSEEAFMGRGVSGCATCDGFFYRGEEVCVVGGGNTAVEEALYLSNIARKVH
                     LIHRRAKFRAEAVMIDKLMHKAQEGKIQLHLDQTLDEVLGDQTGVTGVRTKSVEGEAY
                     AEIPVKGCFIAIGHHPNTDIFQGHLDMKDGYILTRTGSEGFATQTSVPGVFAAGDVQD
                     HVYRQAITSAGTGCMAALDAQRFLESLS"
BASE COUNT         1132 a         2338 c         2410 g         1181 t
ORIGIN      
        1 caccaaggcc acgctcggcc tgaaggtggt cgacctgagc gacgccgagc gggcgcgcct
       61 gcatgtcaag tcgggcgtgc gcgtggacag cgtcgagggg gcggccgcgc gcgccggcgt
      121 gcaggagggc gacgtcatcc tgcagatcgg caacgtgccg gtcagctcgg ccaagcagtt
      181 cgtcgcgacg gtggccaagc tcgacaagtc caagtccatc gccgtgctcg tgcggcgcgg
      241 ggattcggcc aattacctcg tgatccgtcc gagcaagtaa aatgctctga tcggcgagcg
      301 gcccgcggct gcctcgcctg ccggcccggt gattcgcgcc atgcgagttt ccgggccgcg
      361 tgcattggga agtccggcgc cgcccgcgct ttccgcacct ccttccccat ctctttcgca
      421 ggtcacccag atggcacagt acgtattcac gatgaatcgc ctcggcaaga tcgttccgcc
      481 gaagcgagag atcctcaagg gcgtgtcctt gagcttcttc cccggcgcca agatcggcgt
      541 gctcggcctg aacggctcgg gcaagtccac gctgctcaag atcatggccg gcgtcgacaa
      601 ggagttcgag ggcgaggcga ccccgatgcc cggcctgaac atcggctacc tgccgcagga
      661 gccgcagctc aaccccgacc agaccgtgcg ccagtcggtg gaagagggca tgggcggcgt
      721 gctggccgcc aagaagcgcc tcgaggaggt ctacatcgag tacggcaacg aggacgccga
      781 cttcgacaag ctggccgcgg agcaggccga gctcgaggcc atcatctcgt cggccggttc
      841 cgacaacgtc gagcaccagc tcgagatcgc cgccgacgcg ctgcgcctgc cggcgtggga
      901 cgccgtcgtc ggcaacctgt ccggcggcga gaagcgccgc gtggcgctgt gccgcctgct
      961 gctgtccaag cccgacatgc tgctgctcga cgaacccacc aaccacctgg acgccgaatc
     1021 cgtggactgg ctggagcagt tcctgcagcg cttcggcggc accgtcgtgg ccatcaccca
     1081 cgatcgctac ttcctggaca acgccgccga gtggatcctg gaactggacc gcggccgcgg
     1141 catcccgtac aagggcaact actcggagtg gctgctgcag aaggagcagc gcctggagca
     1201 ggagcagaag accgaggacg cgcgcaccaa ggcgatgaag aaggaactcg agtgggtgcg
     1261 ccagaacccc aagggtcggc aggccaagtc caaggcccgc atggcgcgct tcgaggaact
     1321 gtcggacttc gactaccaga agcgcaacga gaccaacgag atcttcatcc ccgtggcgga
     1381 ccgcctgggc aacgaggtca tcgagttcac gaacgtcagc aagggcttcg gtgaccgcca
     1441 gctgatcgac aacctcagct tcaaggtgcc cgcgggcgcc atcgtcggca tcatcggccc
     1501 gaacggcgcc ggcaagtcga cgctgttccg catgatcgcc ggcaaggaac agcccgattc
     1561 gggcacggtg tccatcggca agaccgtgaa gatggccttc gtcgaccagt cccgcagcga
     1621 gctgcaggac gccaagaccg tgtgggagga cgtctccggc ggcctcgaca acctgacggt
     1681 cggcaagttc gtgatgccgt cgcgcgccta catcggccgc ttcaacttca agggcaacga
     1741 ccagcagaag ctcgtcggac agctgtccgg cggcgagcgc ggccgcctgc acctggccaa
     1801 gacgctgatc cagggcggca acgtcctgat gctggacgaa ccgtccaacg acctcgacgt
     1861 cgaaaccctg cgcgcgctgg aagacgcgct gctggaattc gccggctcga tcatggtgat
     1921 cagccacgac cgctggttcc tcgaccgcat cgccacccac atcctcgcct gcgaaggcga
     1981 ttcgcagtgg gtgttcttcg acggcaacta ccaggagtac gaggccgaca agaagaagcg
     2041 cctcggcgag gaaggcgcgc gtccgaagcg cgtgcgctac aaggcgctca agtaagcgcc
     2101 caccacgccc ctgcgttgcg ggggcgcctt ccggcggccg cggccgccgg aaacacccca
     2161 tttttgggca acgccaggat gcagcgttgt cagtacactc ggtcgagcag acggggaacc
     2221 accccgacgc accgacaaat gggggagtga tgaccatcaa tgcactgaag tgcgccgccg
     2281 cggcggccct gctggccacg gccggcgttg cgggcgcggc cacggtcacg ttcgattcgc
     2341 tgacgaatcc gggcatctgg gcctacaccg gcgacacgta taccgagcag ggcatgtcgt
     2401 tcgcggccac cgtgcgctcc aacggctaca cgctgtatgc gtacggcctg gaaagcccgt
     2461 tcaacgccga cccggacggc gccacgctgc accaggagtt cgagggtgac tacggcatct
     2521 tcgtgtcccc ggccggcgcc ggcggcacgt tcacgctgac ctcgttcgac ctggccaatt
     2581 cctggaacga cgcctcgggc ggcaacgtcg acttccgcta cgtcgacgcc acgggcgcgc
     2641 accacgagac gctgacgccg gcggaccaga tcggcctgca gacgttcacg ttcgacctga
     2701 cgggcgtccg ctcgttctcc ctctggagca acaactacca gctggacaac gtccaggtca
     2761 acgacatcat cccggtgccg gaaccctcgt cgtacgcgtt cctgctggcg ggcctggccg
     2821 gcatcatcgg cctgtcgcgt cgccgcaaga actgagcgtc gacacgacag cgagccagag
     2881 ccgcccgcga ggcggctttt tcatgcgcgt cgcgtgcgtc acgcgcgcgc gggccggcgg
     2941 gtgcgcggcg ctcaatcgaa gcgaagcgac acgccggtca cgaacagcgt gtcgttgcgc
     3001 cccaggccct ggccgggccg gctgtcatag cgatgcgaca gcgtggccgt cagcgccagc
     3061 tgcttggtca tcgccaccga caggccggca tcgaacaccg tgcgcacgtc gccggtgtgg
     3121 cgcaggttgg gatagacggt cagcttctgc ttgaacgtgg tcgtgtcgct gagcttgttg
     3181 ttcgactcct cggccagcag cagctcggcg tggtcgtagc ggctgcgggt ctcgtcctcg
     3241 atcacctgcg ggtgccggaa gcgatccatc gtgtaggcga ggccggcgga caggtcgaag
     3301 ctctggtcgg gcttcttgac cacgtggtag ccgatgcccg agccgagcgt cgtgcgcagc
     3361 gacaggtcgc tgggcacgtc gtgcagcagg tcgagctcgc cgaagccgaa ccactggggc
     3421 gtgaagtcgc tcgcgtagat cgtgctgccg ccgacgtggt tggccgtggt ggcgccgttg
     3481 ctgcgcgcgt actgcgccgc gccgttgacg gcccacttgt cgctgtcggt cagcttgatg
     3541 atgtcggcgc cgagcgaggc gctcttggcg tccgtgttgc cggtggcgat gctcgcgccg
     3601 gcggtgaaca gcgaccgcca ctggccgtcg ggtttctggg tgacctgggc gagggccagc
     3661 agcggcacgg tggccgcgca cagcgcgaca agacgggcaa gagagcggat ctgcatctgg
     3721 acgactccat cggggaacaa gggatcgcgc ggcgcggcag gtcgcgcgcc cgcggcggtg
     3781 tcgtcatgca agagatgttt ccgaccacgg gccgcctcca gcggcctgcc cggaccggcc
     3841 tcaaggcgcc gcggtcacgg cgtagccgtc gagggccacg tagaagcgct ggccgtgttc
     3901 gttctccacc agcaggcggc gctccgcggc ctgcagctcg aggtggacga agaacacgtc
     3961 ctgcacgtcg gcctcgatgt cgcgatcgaa cgcgaccgcg tacaccttga ccgccgccag
     4021 gcgcttgccg gtggcgacgt cggtgacggc gaggtagccg gtgcgctggt cgaggtgctc
     4081 gtcctggccg ttgatgatct gctcgtacct gcggccttcg aactcgaccg ggtcgacatc
     4141 gggcggaccg atgcggctga agctcatgtc gtcgtcggac atccgtttct ccttgggttg
     4201 gcccggcgcc gcgcagccgg ccacgcccgc caccgcggtc gccgcggcga ctcggagcag
     4261 ggcgcggcgc gcggcgtcga cgcgggacaa ggggttgctc aattccagtt cttggcctgc
     4321 ttgaccagct tgccgccggt gccgccgagc gcgcgtagac gctcgccgtc ggtcagggcg
     4381 ccggcgcagt ccgccgcgaa gaaggcccac gagtcggcgt tggtcgcagc cttgtcggcc
     4441 gcgatcttcg cgcccggccc gatgccccgg aaggcatagc cgttgtcctg cgtgccgacc
     4501 tcggtgtgcg tgcactcgtg gaccagcacc cgggcccagt tcttcttcat gtcgtgcagc
     4561 accgagatgt cgtagttctc gaacagcgcc ttctcgatgt agatggtgcg cgggcgctcg
     4621 aacttgccgt ccgtgcggcg aacgaacgcc tcgaccgtgt tcttcttcgc gtcgctgcga
     4681 tacatgggca tgtcagtgat caccacctga ttgtggttca gcgcgaacgc gatcttcttg
     4741 aagccggcga gcagggcggc gatcgtcttg tcgacgtccg tggcggccgt gtccgacgcg
     4801 gcgaaccagc gttcgacgat cgcgcgcgac gccggcgtcg acgcggcgtt gcccagcacc
     4861 accttggccg actcgcacca gctgagcccg agctgcatcg ccgcggccag gtggtccttc
     4921 gtttcctcgg agaactgctc cgtgacgtcg gcgagcttgg ccttgacctt ggtggcgtcg
     4981 gccttgacgt ccagcagttc gtcggaggcg aacttggcat acgaactcgg cgtggagacg
     5041 acccacacct gctggctgcc gcgcgcgccg gcgaggtaga gatggcgcag catcttgagg
     5101 ctggcggcca tcttcacggc atcgtccttc acgagacccg tggcgggaac gcccgcggcc
     5161 tccatgatct tgtcggcttc cttgaacttc agcgcgacct cgcggaagtg actgaccttg
     5221 ccgtagaagt tcagctcggg gccttcggcg ccgagcgcct tggcaagatc gagcaggccg
     5281 ggcaaggcgc cgccgaagtc ctggccttcc agcgtctcgc ggcacaggcg gtattcgtcc
     5341 gcaaatccgg tcgatggcat tcgatgttct ccctgtcgtg ccgacgcgtg cgcgcggcgg
     5401 caaggacagg aggatcccgg atcgacgccg agaccgcaag ggcccggatg ggggatacgg
     5461 cgggcgcatc cggcgtccgg aaacgaaaaa gccgcccgga ggcggctttc gcgacttcgg
     5521 agcgcggcgt cgcgtcgcag gctccggcaa gctcagcgca gcttgatttc cttgtacagg
     5581 acgtgcttgc gcgccttcgg atcgaacttc atgaactcga gcttttcagg cgtcgtcttc
     5641 ttgttcttgt tcgtggtgta gaaatgaccc gtacccgcgg tcgattccag cttgatcttt
     5701 tcgcgtccac ccttggcagc catggtgtgt atctccggtg atgtttagag ttcgcccttg
     5761 gcgcgcaggt cggcgaggac ggcgtcgatg ccgttcttgt cgatcagtcg cagcgcggcc
     5821 gtcgtgatgc gcaggcgcac gaaacggttc tcgctctcga cccagatgcg gcggttctgc
     5881 aggttcggca ggaaacggcg cttggtgcgg ttgttggcgt gggaaacgtt gtttcccgtc
     5941 atcgggtgct tacccgtcac ttggcagaca cgagccatga agctctcctt gattcgaccg
     6001 ggcgcccaat gcgcaccggg ttggaaagcc tgcacatacg ccaagagggc ggctggcttc
     6061 caagggaact gcggattcta ccccaaatgg cgttgatcgc caaacgcagg cctgccggaa
     6121 cgcggcagcc cctggccgat cggcttcagc tcagcgattc gaggaagcgc tgggcatcca
     6181 gcgcggccat gcagccggtg ccggccgagg tgatcgcctg gcggtacacg tggtcctgca
     6241 cgtcgccggc ggcgaagacg cccgggacgc tggtctgggt ggcgaagccc tcgctgccgg
     6301 tccgggtgag gatgtagccg tccttcatgt ccaggtggcc ctggaagatg tcggtgttgg
     6361 ggtggtggcc gatggcgatg aagcagccct tgaccgggat ctcggcgtag gcctcgccct
     6421 cgacggactt cgtgcgcacg ccggtgaccc cggtctggtc gcccagcacc tcgtccaggg
     6481 tctggtccag gtgcagctgg atcttgcctt cctgggcctt gtgcatcagc ttgtcgatca
     6541 tcaccgcctc ggcgcggaac ttggcgcggc ggtggatcag gtgcaccttg cgggcgatgt
     6601 tggacaggta cagcgcctcc tcgacggccg tgttgccgcc gccgaccacg cagacttcct
     6661 cgccgcggta gaagaacccg tcgcaggtgg cgcagccgct gacgccgcgg cccatgaacg
     6721 cctcctcgga cggcaggccc aggtacttgg ccgaggcgcc ggtggcgatg atcagggcgt
     6781 cgcaggtgta ggtgcccgag tcgccggtca gcctgaacgg gcgctggctc aaatcgacct
     6841 tgttgacgtg gtcgaacacg atcctggtgt cgaaacgctc ggcgtgggcc aggaaacggg
     6901 ccatcaggtc ggggccctgc acgccggcga cgtcggccgg ccagttgtcc acctcggtgg
     6961 tcgtcatcag ctgtccgccc tgggccaggc cggtgaccag catcggcttg aggttggcgc
     7021 gggccgcata aagggccgcc gtgtaccctg ccgggccgga a
//
LOCUS       DATCRO010000229         6376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_58689, whole genome shotgun sequence.
ACCESSION   DATCRO010000229 DATCRO010000000
VERSION     DATCRO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58689"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1995
                     /locus_tag="VIN75_06190"
     CDS             124..1995
                     /locus_tag="VIN75_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8973789.1"
                     /translation="MPRRTTPRAAAAALALAARTVLAQGDAPDLGDLGIEELGRVPVT
                     SVSGRPEPVQDAAASVFVITAEDIRRSSATSLPEALRLAPTLQVARINATQYAISARG
                     FNNAIGNKLLVMIDGRTVYSPLFSGVFWDAQQVPLADVERIEVVNGTGGALWGANAVN
                     GVINVITRSAQATPGVLASGFAGATANEATLRYGGKLGDAAWRVYAMQSDRGRTTLQS
                     GASGDDHQDRQQAGFRVDGGRPADAWTVQGDLYQAGGDGHAPASAQLTGANLLARWRH
                     DEGDGANWQLQAWFDQAGRDDVVDFHDHARTLDLQFNHEPALGPDHRCIWGFGYRHAI
                     DETDPTPIVGFTPRARQLRWANAFLRDDFRVTQRLRVIAGARIETNVYTGTELLPTLR
                     TVWDLGPAGSMWALASRAVRAPARLDRDIQIPSTPPYLIDRGENFQSEIAHVFEVGLR
                     GQQPSGFGYSVALFHTDYSRLRAGHDAPTPVENLAWGETTGMELTASADLARDWRVSL
                     GWTELREALRAAGNAGPNSVANLGDDPRRQIQLRSTLRPARNVDVDLTLRHVAALPAP
                     AVPAYTVVDARLAWSPRPDLELSLLAQDLGRRHVEFDPSNSSRFGPRAFLKIDWRTP"
     gene            2175..4349
                     /locus_tag="VIN75_06195"
     CDS             2175..4349
                     /locus_tag="VIN75_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973790.1"
                     /translation="MTFLTPSYDLRVVAASFFIASFASYVALDLTSRVRTSDRRLARR
                     WWASGSIAMGTGIWSMHFIGMLAFSLPITLGYTAGMTAISWLAGVSVSAVALGAVMRH
                     DMAWRHLAGGAVAMGVGICTMHYSGMAALQMTPGIVWNWPLVGASALIAVSASAVALN
                     VFKWFRGGKRSPVWQLVAALVLGAAVCGMHYTGMGAAGFPSDAVCLSAGGLNGERLGM
                     LVTVSSLMLLTMTLYASTVDARMEGRTVRLAASLKTANDELQAANEALKQRALTDPLT
                     GLPNRVLFEDRLAQACKRRDRAAAAASKAAGAREVPCFAVLFVDLDGFKPVNDVLGHA
                     AGDQVLQEAARRLRVTGRDNDTVARIGGDEFLLLAEGLGGVADASALADRLVRRLARA
                     FEVDGRRVHVSASIGVVVYPDHGQREQLVTRADAAMYAAKRAGGNTRAVFEPRMDEAG
                     AERLNLHSELRMAIERGQLKLHYQPKIDSTTGQIRGVEALLRWTHPERGNVSPVVFIP
                     IAERFGLINEIGNWVIDEVCRQLHEWADEGMRTRVAINLSVHQLRQDDLVDRIEAALK
                     RHGLDPSQLLCEVTESVAMGDPDTLQRVFDGLARIGVFISIDDFGTGYSCLINLRQLP
                     AAQLKIDRSFINDIETSKDALAIVSAVVNLAHALGLKVVAEGVETAGQRDILIGMNCD
                     EMQGFYFARPMAAESLHDWFLGNKPEGAVDFAPSAMLEHIPT"
     gene            4555..5451
                     /locus_tag="VIN75_06200"
     CDS             4555..5451
                     /locus_tag="VIN75_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8973791.1"
                     /translation="MPFRARLRSSPRRRRLRHPLVGLVLALATAVRAAVPDAPPPPDA
                     PIPAPPSTVTPAAAPRLPDCSRPLTLGLHEHGLLYASQTGEGIDKDVADEMSRRSGCR
                     LTLTMLPRARIWQLIESGALDFTLSGIANAQRDRFATFAWYVSNKYYLLVRRDADVRS
                     VAEFRRKRALRVGLIRSFRYGEQANAFVDALEAQDRVTYAGSLDPLYTILLDDGIQAM
                     IIEPFDFPAIAGAQLKAQTAILDFGDPPVPHGLVMSRKSLAPAEQQAWRDIIQSMRSD
                     GTMRRIFEKYFPVDLARQMTQF"
     gene            5471..>6376
                     /locus_tag="VIN75_06205"
     CDS             5471..>6376
                     /locus_tag="VIN75_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEY8973792.1"
                     /translation="MKRALPPLIALFVGAIMLAFTIGLCVHERDNEQARLRAVFDANA
                     RQAAGRIEQRIANYEQMLRGVQGLLSADVGIDRLGFEAYVDTVMAGPDAAGLQSVAFS
                     MLVGPQELADFVGHQRMTGRSEFTVRPDGVRDLYAPIILVAPDSVANRKVVGLDSLSD
                     ATRRAAMLQARDSGNATLTALVQLRADAGAEPKPGCLIYLAVYRKGASVNSVAERRAN
                     AIGWVRAAFRVGDLMSTLYGEQSPGLVVRVHDGADTGAASLMYRSDGDGVGAGDEQIA
                     HARFQAQEYVTLAGHTWTVQLSSLPE"
BASE COUNT          901 a         2301 c         2257 g          917 t
ORIGIN      
        1 ggagcgcgcg acggcgtccg gcttcgaccg ccacttcgtc aagcccgtac cgccaggggt
       61 attgatcgag gcgctcgggg acgcgctggg aatggaggga tcatgacgaa gaatcgacaa
      121 accatgccac gccggacgac accccgggcc gcggctgcgg cgctcgcgct tgcggcccgc
      181 accgtgcttg cgcagggtga cgcgcccgac ctgggcgacc tcggcatcga ggagctgggc
      241 cgcgtccccg tcacatccgt cagcgggcgg cccgaaccgg tgcaggatgc cgcggcgtcg
      301 gtcttcgtca tcacggccga agacatccgg cgctcgtcgg ccacgagcct gcccgaggcg
      361 ctgcgcctgg cgccgacgct gcaggtggcg cgcatcaacg ccacgcagta cgccatcagc
      421 gcgcgcgggt tcaacaacgc catcggcaac aagctgctgg tgatgatcga cgggcgcacc
      481 gtgtactcgc cgctgttctc gggcgtgttc tgggatgccc agcaggtgcc gctggccgac
      541 gtcgagcgca tcgaggtggt caacggcacc ggcggcgcgc tgtggggcgc gaacgcggtc
      601 aacggggtga tcaacgtcat cacgcgatcg gcgcaggcta cgccgggcgt gctcgcatcc
      661 ggcttcgcgg gcgcgacggc caacgaggcg acgctgcgct acggcggcaa gctcggcgac
      721 gcggcctggc gcgtgtacgc gatgcagtcc gaccgcggcc ggacgacgct gcagtccggc
      781 gcctccggcg acgaccacca ggatcgccag caagcgggtt tccgggtgga cggcggacgc
      841 ccggccgacg cctggacggt gcagggcgac ctctaccagg ccggcggcga tggccatgcc
      901 ccggcctcgg cgcagctgac cggcgcgaac ctgctcgcgc gctggcgcca cgacgagggc
      961 gacggcgcga actggcaact gcaggcctgg ttcgaccagg cgggccgcga cgacgtcgtc
     1021 gatttccacg accacgcgcg cacgctcgac ctgcagttca accacgagcc ggcgctgggt
     1081 cccgaccatc gctgcatctg gggattcggc tatcgccacg ccatcgacga gaccgacccg
     1141 acgcccatcg tcggcttcac gccccgcgcg cgccagctgc gctgggccaa cgcgttcttg
     1201 cgcgacgact tccgcgtcac gcagcgcctg cgcgtcatcg ccggcgcgag gatcgagacc
     1261 aacgtgtaca ccggcaccga gctgctgccg accttgcgca cggtgtggga cctgggcccg
     1321 gccgggtcga tgtgggccct ggcctcgcgc gccgtgcgcg cgccggcgcg gctggatcgc
     1381 gacatccaga ttccttccac gccgccatac ctgatcgacc ggggcgagaa tttccagtcg
     1441 gagatcgcac acgtcttcga ggtgggcctg cgcgggcagc agccctcggg attcggctat
     1501 tccgtcgcgc tgttccacac cgactacagc cgccttcgcg cgggccacga cgcgccgacg
     1561 ccggtggaga acctcgcgtg gggcgagacc accggcatgg agctgaccgc cagcgccgac
     1621 ctcgcgcgcg actggcgcgt gtccctgggc tggacggagc tgcgcgaggc gctgcgcgcc
     1681 gccggcaacg cgggcccgaa ctcggtggcc aacctcggcg acgatccgcg tcgccagatc
     1741 cagctgcgct cgaccctgcg tccggcgcgc aacgtcgacg tcgacctgac gctgcgccac
     1801 gtcgccgcgc tgcccgcgcc ggcggtgccg gcctacacgg tggtcgacgc gcgcctggcg
     1861 tggtcgccgc ggccggacct ggagctgagc ctgctggcgc aggacctggg ccgccggcac
     1921 gtcgaattcg acccgtcgaa ctcgtcgcgc ttcgggccgc gcgcgttcct caagatcgat
     1981 tggcgcacgc cctgaggccg gcgaaccgca cggtgcggcg catcggcggc gccggcgaca
     2041 gaaatgccgg cccgcgccgt gacttcctcc ttccgccagg ctcatgcggg gcttaaagcc
     2101 gagcggaatc ccgtcgacac tgacgatagc gcgccgaccg gtgccgcgtg ttccaagctc
     2161 cgggggatct gggcgtgact ttcctcacac cgtcgtacga cctgcgcgtg gtcgctgcgt
     2221 cgttcttcat cgccagtttc gcatcgtacg tcgcgttgga cctgacctcc cgggtgcgca
     2281 cgtccgatcg ccggctggca cggcgctggt gggccagcgg atcgatcgcg atgggcaccg
     2341 gcatctggag catgcatttc atcggcatgc tcgcgttcag cctgccgatc acgctcggct
     2401 acacggcggg catgacggcg atctcctggc tcgcgggcgt gtcggtctcg gcagtggcgc
     2461 tgggtgccgt catgcggcat gacatggcct ggcggcacct cgccggcggc gccgtcgcga
     2521 tgggcgtggg catctgcacc atgcactatt ccggcatggc ggcgctccag atgacgccgg
     2581 gcatcgtctg gaactggccg ctggttggcg cgtcggcgtt gatcgcggtg agtgcctccg
     2641 cggtcgccct caacgtcttc aagtggtttc gcggcggcaa gcgaagcccc gtctggcagt
     2701 tggtggcggc gctggtgctc ggcgcggcgg tctgcggcat gcactacacc ggcatggggg
     2761 cggcgggatt tccgagcgac gcggtctgcc tgagcgccgg cggcctgaac ggcgagcgcc
     2821 tgggcatgct cgtgaccgtt tcgtcgctga tgctgttgac gatgaccctg tacgcctcga
     2881 ccgtcgacgc gcgcatggaa ggtcgcaccg tgcgcctggc ggcatcgctg aagaccgcca
     2941 atgacgaact gcaggcggcc aacgaggcgc tcaagcagcg cgcgctgacc gacccgctga
     3001 cgggcctgcc caaccgcgtg ctgttcgagg accgcctcgc gcaggcctgc aagcgccgcg
     3061 accgcgccgc ggcggcggcg agcaaggccg ccggcgcgcg cgaggtgccg tgcttcgcgg
     3121 tactgttcgt cgacctcgac ggcttcaagc cggtcaacga cgtcctgggg cacgccgccg
     3181 gcgaccaggt gctgcaggag gccgcgcgcc gcctgcgcgt gacgggccgc gacaacgata
     3241 cggtggcccg catcggcggc gacgagttcc tgctgctggc cgagggcctg ggcggcgtgg
     3301 cggacgcgtc cgcgctggcc gaccgcctcg tcaggcggtt ggcgcgcgcg ttcgaggtgg
     3361 acggccgccg cgtgcacgtg tcggcgtcca tcggcgtggt ggtctacccc gaccacggcc
     3421 agcgcgagca gctcgtgacg cgcgccgacg ccgcgatgta cgcggccaag cgcgccggcg
     3481 gcaacacgcg cgccgtgttc gagccacgca tggacgaggc cggcgccgag cgcctgaacc
     3541 tgcacagcga gctgcgcatg gccatcgagc gcggccagct caagctgcac taccagccca
     3601 agatcgactc gaccaccggc cagatccgcg gcgtcgaggc gctgctgcgc tggacccatc
     3661 ccgagcgcgg caacgtcagc ccggtggtct tcatcccgat cgccgagcgc ttcggcctga
     3721 tcaacgagat cggcaactgg gtgatcgacg aggtctgccg ccagctgcac gaatgggccg
     3781 acgaaggcat gcgcacgcgc gtcgcgatca acctgtcggt gcaccagctg cgccaggacg
     3841 atctcgtcga tcgcatcgag gcggcgctca agcgccacgg gctcgacccg tcgcagctgc
     3901 tgtgcgaggt gaccgaatcg gtggccatgg gcgacccgga cacgctgcag cgcgtgttcg
     3961 acggcctcgc gcgcatcggc gtgttcatct ccatcgacga cttcggcacc ggctactcgt
     4021 gcctgatcaa cctgcgccag ctgcccgcgg cgcagctcaa gatcgatcgc agcttcatca
     4081 acgacatcga gaccagcaag gacgcgctgg ccatcgtcag cgccgtcgtc aacctggcgc
     4141 acgcgctggg cctgaaggtg gtggccgagg gcgtggagac cgccggacag cgcgacatcc
     4201 tgatcggcat gaactgcgac gagatgcagg gcttctactt cgcccgcccg atggccgccg
     4261 agtcgctgca tgactggttc ctcggcaaca agcccgaagg cgcggtggac ttcgcgccgt
     4321 cggcgatgct ggagcacatc ccgacctgag cgcccttcgc gcccgccgca caggcgcgaa
     4381 ccagaggggg cggcgcgaac ccacccgtgt tgcgcaacgc ccgaccggtg tggggcaaag
     4441 cacgaagctc ggggaatgcg cgtgttccaa tgggcgggtc gtctgtaaca tcggcgttgc
     4501 gccggttaca gggagtcccg gcgtccccgc cgcttccagg gtcaccgcat cgacatgccc
     4561 ttccgcgccc gtctccggtc gtccccgcgt cgacgccgcc tgcgccatcc gctcgtgggg
     4621 ctggtgctcg cgttggcgac ggccgtccgc gcggcggtgc ccgatgcgcc gccgccgccc
     4681 gacgcgccga tcccggcgcc cccgtcgacg gtcacgccag ccgccgcgcc gcgcctgccg
     4741 gactgctcgc gtcccctgac gctgggcctg cacgagcatg gcctgctgta cgcgagccag
     4801 acgggcgagg gcatcgacaa ggacgtcgcc gacgagatgt cgcgccgcag cggctgccgc
     4861 ctgacgctga ccatgctgcc gcgcgcccgc atctggcagc tgatcgagtc gggggccctg
     4921 gacttcacgc tgtcgggcat cgccaacgcg cagcgcgacc ggttcgccac cttcgcctgg
     4981 tacgtgtcca acaaatacta cctgctcgtt cggcgcgacg ccgacgtgcg cagcgtggcc
     5041 gagttccgcc gcaagcgcgc gctgcgcgtg gggctcatcc gcagcttccg ctacggcgag
     5101 caggccaacg cgttcgtcga cgcgctcgag gcgcaggatc gcgtcaccta cgcgggcagc
     5161 ctcgacccgc tctacacgat cctgctggac gacggcatcc aggcgatgat catcgagccg
     5221 ttcgatttcc ccgccatcgc cggcgcgcag ctcaaggcgc agacggccat cctcgacttc
     5281 ggcgacccgc ccgtgccgca cgggctggtg atgtcgcgca agtcgctcgc gcccgccgag
     5341 cagcaggcgt ggcgcgacat catccagtcg atgcgcagcg acggcacgat gcgccgcatc
     5401 ttcgagaagt acttccccgt cgacctggcg cgccagatga cgcagttcta ggacgacggg
     5461 gccggtcttc gtgaaacgcg ccctgcctcc cctgatcgcg ctgttcgtcg gcgcgatcat
     5521 gctcgcgttc accatcgggc tgtgcgtgca cgagcgcgac aacgagcagg cgcggctgcg
     5581 cgccgtgttc gacgccaacg cgcgccaggc ggccgggcgc atcgagcagc gcatcgccaa
     5641 ctacgagcag atgctgcggg gcgtgcaggg cctgctgtct gccgacgtgg gcatcgaccg
     5701 gctcggcttc gaggcctacg tcgacacggt gatggccggc cccgacgcgg cgggcctgca
     5761 gagcgtggcg ttctcgatgc tggtcggccc gcaggagctg gcggacttcg tcggccacca
     5821 gcgcatgacg gggcgctcgg agttcaccgt gcggcccgac ggcgtgcgcg acctgtacgc
     5881 gcccatcatc ctcgtcgcgc ccgacagcgt ggccaaccgc aaggtggtcg gcctggactc
     5941 gctgtccgac gcgacgcggc gcgcggcgat gctgcaggcg cgcgactccg gcaacgcgac
     6001 gctgacggcg ctggtgcagc tgcgcgcgga cgccggcgcc gagcccaagc cgggctgcct
     6061 gatctacctg gcggtgtatc gcaagggggc ctcggtcaat tcggtggccg agcggcgtgc
     6121 caacgcgatc ggctgggtgc gcgcggcgtt ccgcgtcggc gacctcatgt cgacgctcta
     6181 cggcgagcag agtcccggcc tggtggtgcg cgtgcacgac ggcgccgaca ccggcgcggc
     6241 ctcgctgatg taccgctccg acggcgacgg cgtcggcgcc ggcgacgagc agatcgccca
     6301 cgcgcgcttc caggcgcagg aatacgtcac gctggcgggg cacacgtgga cggtgcagct
     6361 cagctcgctg cccgag
//
LOCUS       DATCRO010000230         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_846374, whole genome shotgun sequence.
ACCESSION   DATCRO010000230 DATCRO010000000
VERSION     DATCRO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846374"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VIN75_06210"
     CDS             <1..991
                     /locus_tag="VIN75_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8973793.1"
                     /translation="GRDAGQALMRHGDRQSWLPLDTPGAVRRFLLRTRPALGVLMETE
                     IWPNLLHGAEERGVPMVLANARLSEKSARQGERFAALMRPAAAAVDDVLAQTEDDAER
                     LREAGARRVALSGNLKFDVRPAPALLSRGKRWHAQIGRPVVLAAITREGEEAELMREW
                     GRIQGRPPFLIVVPRHPQRFDEVEALLVANGLRVARRSSWGEAPPPAALKVDAWLGDS
                     LGEMPQYYAASDVALLGGSFMALGGHNLIEAAACGCPIVMGPSTFNFAEAARQAALAG
                     AAWQEPDMAHALRRALQVIGSELQDLASTRALAFAATHKGAADRMADRIVRLL"
     gene            1103..1483
                     /locus_tag="VIN75_06215"
     CDS             1103..1483
                     /locus_tag="VIN75_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY8973794.1"
                     /translation="MPREIVFTDKAPKPPATYSQAVKAAGLVFVSGTAPHDPATGAIV
                     GTTIQEQVRQSLANVAAILEAAGSSMDRIASVNIVLADEDDWAGMNEEYLRWFPSNPP
                     ARSGAKFPTRIPGVKVSISAIAAA"
     gene            1494..2054
                     /locus_tag="VIN75_06220"
     CDS             1494..2054
                     /locus_tag="VIN75_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973795.1"
                     /translation="MQPGRIIFLNGASSAGKSTLARALQAQLDSPFWHWSIDHWLAAG
                     VLPRERLSSGEFDWQAMRPAFFEGFHRSLPALADAGNDLIVEHIVETGDWMRLLVDLL
                     APFDVFCVGVRCPLPELERREQARCDRRVGSAREDDASTHGLCAYDVDVDTTAAPAER
                     LAADVLRAWRARRAPCAFARMAARPR"
     gene            2145..3110
                     /locus_tag="VIN75_06225"
     CDS             2145..3110
                     /locus_tag="VIN75_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8973796.1"
                     /translation="MSTRFCRTAAAGALCAVFTGAASAQDVKVTPIGGIDGEFCAQDR
                     ALVFEDPDGTRLLYDPGHTVAGGGDPRLGRIDVVLLSHMHNDHLGEAHIKAAGAGTCA
                     KPDVSVPTPDSNVVDIALAKKAKIVTGSEMPPFFAARLKKGGGDPANSILARFGGSVT
                     VGGVKIATVAALHSNGVDPAYVGGAAGDAMKAAGIAGDVGPATGYVLRFSNGLVAWLS
                     GDTGILADQQLVIRDYYHAKLAVMNIGDGFTTGPAEAAYVIDDLVKPASVIPSHANEV
                     GTVDGKVREGSKTEAFIKAVQVPVHVPLSGRTMSFDAGGKCVAGC"
     gene            3115..3705
                     /locus_tag="VIN75_06230"
     CDS             3115..3705
                     /locus_tag="VIN75_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973797.1"
                     /translation="MTRFDVQPLTPERWPDLEAIFEARGCSVARGCWCMYYRVSGKET
                     GFTRPGDEQRERSKQALKAMAGDDPPPGLIGYRDGVPVGWISLGPRADYAKLARSPTM
                     KAVDDQPVWSIVCFVVPSEHRKRGVARDLLAGAIRWARRRGVRLLEAYPVDRSHPSAS
                     DAPWFGSKTLFDAAGFEEVARRRPARPIVRLMLAAK"
     gene            complement(3711..>5089)
                     /locus_tag="VIN75_06235"
     CDS             complement(3711..>5089)
                     /locus_tag="VIN75_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8973798.1"
                     /translation="YGQEKLRMSVVRDLRDRHAAQARIHHLAHHDALTGLPNRMSFME
                     RLGQQIEQARAAGKSLALLFVDLDHFKRVNDSLGHLIGDKLLRTVAARISASLRTNDV
                     VARFGGDEFIVLLDDAGRDDVAQVAQKLLRAIELPVEAEGRDLSVTPSVGVALFPHDG
                     QTPTELIKNADTAMYIAKSRGRANCQFFDPAVASAAYDALVLESQLTQALQREEFVLH
                     FQPQVRARDGALAGIEALIRWMHPERGMLRPDAFIALAEQQRVMLPISQWVLRSAARA
                     ALQWRSQGLIEVPIAVNLSSMQFRANGFVDAVAQVLREEGVPGQWLELELTERMLMDD
                     LDDVKRTLQQLKALGMRVSVDDFGTGYSSLAHLKELPIDGMKIDRSFVRDLPHQRGSV
                     AIARAVVQMAQGLQLTVVAEGVENEEQRRFLVDHGCDLLQGELISLPLPADDLARWIV
                     QHQAALSA"
BASE COUNT          694 a         1843 c         1852 g          700 t
ORIGIN      
        1 cggccgcgac gccggccagg ccctgatgcg ccatggcgac cgccagtcgt ggctgccgct
       61 ggacacgccc ggcgccgtgc gccgcttcct cttgcgcacg cggcccgcgc tgggcgtgct
      121 gatggagacc gagatctggc ccaacctgct gcacggcgcc gaggagcgcg gcgtgccgat
      181 ggtgctggcc aacgcccggc tgtcggagaa gagcgcgcgc cagggcgagc gcttcgccgc
      241 gctgatgcgt ccggcggcgg cggccgtcga cgatgtcctg gcccagaccg aggacgacgc
      301 cgagcgcctg cgcgaggccg gcgcccggcg cgtcgcgctg tcgggcaacc tgaagttcga
      361 cgtgcggccc gcgccggcgc tgctgtcgcg cggcaagcgc tggcacgcgc agatcggccg
      421 gccggtggtg ctggccgcga tcacgcgcga gggcgaggag gccgagctga tgcgcgagtg
      481 gggccgcatc cagggccgcc cgccattcct gatcgtggtg ccgcgccatc cgcagcgttt
      541 cgacgaggtg gaggcgctgc tggtggccaa cggcctgcgc gtcgcgcgcc gcagcagctg
      601 gggcgaggcg ccgccgccgg cggcgctgaa ggtcgacgcc tggctgggcg attcgctcgg
      661 cgagatgccg cagtactacg ccgcgtcgga cgtcgcgctg ctgggcggca gcttcatggc
      721 gctcggcggc cacaacctga tcgaggccgc ggcctgcggc tgcccgatcg tgatggggcc
      781 gtccacgttc aacttcgccg aggccgccag gcaggccgcg ctcgcaggcg cggcctggca
      841 ggagcccgac atggcgcacg cgctgcgccg cgcgctgcag gtgatcggca gcgagctgca
      901 ggacctcgcg agcacgcgcg cgctggcctt cgcggcgacg cacaagggcg cggccgaccg
      961 catggcggac cgcatcgtgc ggctgctctg atcctccgcg gcgcgcatcg gtcatcgacc
     1021 tcttgcggcc gggcggcggc gacggcacat tgggcattcc cggctcgatc gatgcggcgc
     1081 cgggccatcc gaggagtcgt ccatgccccg cgagatcgtc ttcaccgaca aggcgcccaa
     1141 gccgcccgcc acctacagcc aggccgtcaa ggcagccggc ctcgtcttcg tgtccggcac
     1201 cgcgccacac gatccggcca ccggcgcgat cgtgggcacg acgatccagg agcaggtgcg
     1261 ccagagcctg gccaacgtcg ccgccatcct cgaggcggcg ggcagctcga tggaccggat
     1321 cgccagcgtg aacatcgtgc tggccgacga ggacgactgg gccggcatga acgaggagta
     1381 cctgcgctgg ttcccgtcca accccccggc gcgctcgggc gcgaagttcc cgacccgcat
     1441 cccgggcgtc aaggtgtcga tctcggcgat cgccgcggcc tgacgcgcgg ccgatgcaac
     1501 cggggcgcat catcttcctg aacggcgcgt cgagcgccgg caagtccacg ctcgcgcggg
     1561 cgctgcaggc gcagctggac tcgccgttct ggcactggtc gatcgaccac tggctcgccg
     1621 cgggcgtcct gccgcgcgag cgcctgtcct cgggcgagtt cgactggcag gccatgcgac
     1681 ccgcgttctt cgagggcttc catcgcagcc tgccggcgct cgccgatgcc ggcaacgacc
     1741 tgatcgtcga gcacatcgtc gagacgggcg actggatgcg cctgctcgtc gacctgctcg
     1801 ccccgttcga cgtcttctgc gtgggcgtgc gctgcccgct gcccgaactg gagcggcgcg
     1861 agcaggcacg ctgcgaccgg cgcgtcggct cggcgcgcga ggacgatgcg agcacgcacg
     1921 gcttgtgcgc gtacgacgtc gacgtcgaca ccaccgccgc gcctgcggag cgactcgcgg
     1981 ccgacgtcct gcgcgcgtgg cgcgcgcggc gcgcgccgtg cgcgttcgcg cgcatggccg
     2041 cgcggccgcg ctgagcgcct gcgcgaccgc gccgggcgcc gtaagatcgc gcgtcgccac
     2101 gcgcgcgaac ggcgcgggcc gcacacgtca gccaggggag tcccatgtcg acacgctttt
     2161 gccggaccgc agccgcgggc gcgctgtgcg ccgtcttcac cggcgccgca tcggcgcagg
     2221 acgtcaaggt cacgccgatc ggcggcatcg acggcgagtt ctgcgcgcag gatcgcgccc
     2281 tcgtcttcga ggatcccgac ggcacgcgcc tgctctacga tccgggccac acggtggccg
     2341 gcggcggcga tccgcgtctc ggtcgcatcg acgtcgtgct gctgagccac atgcacaacg
     2401 atcacctcgg cgaggcgcac atcaaggcgg cgggtgcggg cacctgcgcc aagcccgacg
     2461 tctcggtgcc cacgcccgac tccaacgtcg tcgacatcgc gctcgccaag aaggccaaga
     2521 tcgtgacggg cagcgagatg ccgccgttct tcgccgcgcg cctgaagaag ggcggcggcg
     2581 atccggccaa ctcgatcctg gcgcgcttcg gcggcagcgt cacggtgggc ggcgtgaaga
     2641 tcgcgacggt cgcggcgctg cacagcaacg gcgtcgaccc ggcctacgtc ggcggcgccg
     2701 cgggcgacgc gatgaaggcc gcgggcatcg ccggcgacgt cgggccggcc accggctacg
     2761 tgctgcgctt cagcaacggg ctggtcgcct ggctgtccgg cgacacgggc atcctcgccg
     2821 atcagcaact ggtcatccgc gactactacc atgcgaagct ggccgtcatg aacatcggcg
     2881 acggcttcac caccggtccc gccgaggccg cctacgtcat cgacgacctc gtcaagccgg
     2941 cgtcggtgat cccctcgcac gccaacgagg tcggcaccgt cgacggcaag gtgcgcgagg
     3001 gcagcaagac cgaggcgttc atcaaggccg tgcaggtgcc ggtgcatgtg ccgttgtcgg
     3061 gcaggacgat gtcgttcgac gccggcggca aatgcgtggc ggggtgctga gcccatgacc
     3121 cgattcgacg tccagcccct cacgcccgag cgctggcccg acctcgaggc gatcttcgag
     3181 gcccgcggct gctcggtcgc gcgcggctgc tggtgcatgt actaccgcgt cagcggcaag
     3241 gagaccggct tcacgcggcc cggcgacgaa cagcgagagc gcagcaagca ggcgctgaag
     3301 gcgatggcgg gcgacgatcc gccgccgggc ctgatcggct atcgcgacgg cgtgccggtg
     3361 ggctggatct cgctgggccc gcgcgcggac tatgccaagc tcgcgcgctc gccgaccatg
     3421 aaggccgtcg acgaccagcc cgtgtggtcc atcgtgtgct tcgtggtgcc gtcggaacat
     3481 cgcaagcggg gcgtcgcgcg cgacctgctg gccggcgcga tccgctgggc gcgccggcgc
     3541 ggcgtgcggc tgctggaggc ctacccggtc gacaggtcgc acccgtccgc gagcgacgcg
     3601 ccgtggttcg gctccaagac gctgttcgac gcggccggct tcgaggaggt ggcccggcgc
     3661 aggccggcgc ggccgatcgt gcggctgatg ctggccgcca agtagggcgc tcaggcgctc
     3721 agcgccgcct ggtgctgcac gatccagcgc gccaggtcgt cggccggcag cggcaggctg
     3781 atcagctcgc cctgcagcag gtcgcagccg tggtcgacga ggaagcgccg ctgctcctcg
     3841 ttctcgacgc cttcggccac caccgtcagc tgcaggccct gggccatctg caccacggcg
     3901 cgcgcgatgg ccaccgagcc gcgctggtgc ggcaggtcgc gcacgaacga gcggtcgatc
     3961 ttcatgccgt cgatcggcaa ctccttcagg tgcgcgagcg acgagtagcc ggtgccgaag
     4021 tcgtcgaccg acacgcgcat gcccagcgcc ttgagctgct gcagcgtgcg cttgacgtcg
     4081 tccaggtcgt ccatgagcat gcgctcggtg agctccagct ccagccactg cccgggcacg
     4141 ccttcctcgc gcagcacctg ggccaccgcg tcgacgaagc cgttggcgcg gaattgcatg
     4201 ctcgagaggt tgacggcgat gggcacctcg atcagcccct gcgaacgcca ctgcagcgcc
     4261 gcgcgcgccg cgctgcgcaa cacccactgg ctgatcggca gcatcacgcg ctgctgctcg
     4321 gccagcgcga tgaacgcgtc gggccgcagc atgccgcgct cgggatgcat ccagcggatc
     4381 agcgcctcga tgcccgccag cgcgccgtcg cgggcgcgca cctgcggctg gaagtgcagc
     4441 acgaactcct cgcgttgcag cgcctgcgtg agctggctct cgagcaccag cgcgtcgtag
     4501 gcggcgctgg cgacggccgg gtcgaagaac tggcagttgg cgcggccgcg cgacttggcg
     4561 atgtacatcg ccgtgtcggc gttcttgatc agctcggtgg gtgtctggcc gtcgtgcggg
     4621 aacagggcca cgcccaccga cggcgtcacc gacaggtcgc gcccctcggc ctcgaccggc
     4681 agctcgatgg cgcgcagcag cttctgcgcc acctgcgcca cgtcgtcgcg gcccgcgtcg
     4741 tccagcagta cgatgaactc gtcgccgccg aagcgggcga cgacgtcgtt ggtgcgcagg
     4801 ctggccgaga tgcgcgcggc cacggtccgc agcagcttgt cgccgatcag gtggcccagg
     4861 gaatcgttga cgcgcttgaa gtggtcgagg tcgacgaaca gcagcgccag cgacttgccc
     4921 gccgcgcgcg cctgctcgat ctgctggccc aggcgctcca tgaacgacat gcggttgggc
     4981 aggccggtga gcgcatcgtg gtgggccagg tggtggatgc gcgcctgcgc ggcgtggcgg
     5041 tcgcgcaagt cgcgcaccac cgacatgcgc agcttctcct gcccgtaca
//
LOCUS       DATCRO010000231        12918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1105164, whole genome shotgun sequence.
ACCESSION   DATCRO010000231 DATCRO010000000
VERSION     DATCRO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105164"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VIN75_06240"
     CDS             complement(<1..2045)
                     /locus_tag="VIN75_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY8973799.1"
                     /translation="MNYALFDDAGKFLAGRVMSEADSSIQVELESGKRVKVKSSHVLL
                     RFEKPQPAELIAQGQALAREIDLDLAWEFASEAEFGFADLARDYFDASAGPVQQAAAL
                     FRLFEAPHYFRRMAKGVFRKAPEETVKAALLGIERKRLQALQIQEWAGELVAGTTPQP
                     IRDQLYKILFRPDKNAPEYKAVVEASKQSQRSPLDLLKAAGAITSPYQFHWRRFLFEE
                     FGKGTGFPPLEAPPVKDDLPVAEVAAFSIDDSQTTEIDDALSVRGLGTGTVVFGVHIA
                     APGLAILPDSPVDKVARERLSTVYMPGWKITMLPDAIVEKYTLAEGRDCPALSLYVTF
                     DEATLEVKGSETRVERVPIVANLRHDKLDAVVTEQSLLGEAPADYAFATELAFTFRLA
                     RHLKALREVVRGKPENFNRPDYNFRLQAADGRSPTSEPAGDETVTITERRRGAPLDLI
                     VSEAMILANSTWGGWIAEMGVPGIYRSQASLAPGVKVRMGIKPAPHAGMGVAQYTWAT
                     SPLRRYVDLVNQWQIIACVRNGRMAPLSAPFKPKDATLFSIISGFDTAYSAYNGFQTG
                     IERFWTLRWLQQNGVTELDAAVLKDGLVRANTLPLVFKAAGCDSLPRGTHVRVRVDGV
                     DLLTLDVHARLVARLDDEAATDEAAAEDADDAEEASAGPLTLAIDVTEETPPAEGAAT
                     "
     gene            complement(2238..2648)
                     /locus_tag="VIN75_06245"
     CDS             complement(2238..2648)
                     /locus_tag="VIN75_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8973800.1"
                     /translation="MTSPVATIPAAPSSAALPHFEASLQFETDCWDVHHSLATGQRDF
                     VLLDVRSAETYAAGHVPGALHLPHGKITEGKMREFPEGTLFVTYCAGPHCNGATRGAI
                     RLAGLGRPVKIMIGGVTGWLDEGFALESGRQTAA"
     gene            2739..3695
                     /gene="ftrA"
                     /locus_tag="VIN75_06250"
     CDS             2739..3695
                     /gene="ftrA"
                     /locus_tag="VIN75_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HEY8973801.1"
                     /translation="MKNHLVAALAYDGLCTFEFGCAVEVFSLERPELGVDWYRFAACA
                     ESRGTVRAAGGISVQVPHGLATLARAGTIVIPGWQGVDVAPSPVLLRRLVAAHERGAR
                     IATICSGVFVLAATGLLDGKAATTHWRYADLLQQRHPRIDVRPNSIYVDEGPLLTSAG
                     SAAGLDMMLHLVRRDHGPRVANQVAQRLVVPPHRLGDQAQFVARPLPQDEAARLARLM
                     DFVRAHPAQDWTLASLADRAAMSVRTLQRQFQQSLGMSPVDWLVRERVEVARQLLETS
                     TRPLAHVSTLAGFGSEESFRRHFRLATGVSPLAYRRQFGVAA"
     gene            complement(3699..4442)
                     /gene="fabG"
                     /locus_tag="VIN75_06255"
     CDS             complement(3699..4442)
                     /gene="fabG"
                     /locus_tag="VIN75_06255"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8973802.1"
                     /translation="MRLSDKIAIVTGAAQGIGLATALKFAREGASVVVCDVHDVDAAV
                     AAVNAVSVAGAACIGQAVDVTDRAQVDAMVAAVKARFGRIDVLVNNAGITRDARLQKM
                     TIEQFDAVIDVNLRGVFHCTQAVADTMVAQGAGSILNASSVVGLYGNFGQTNYAATKF
                     GVIGFTKTWSRELGPKGVRVNAVAPGFVETPILATVPDKVLQHMREQVPLHRLGKPEE
                     IANVYAFLASDEASYVNGAVIEVSGGMTV"
     gene            complement(4486..5151)
                     /locus_tag="VIN75_06260"
     CDS             complement(4486..5151)
                     /locus_tag="VIN75_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY8973803.1"
                     /translation="MTARLAPLPAPEGLRPARSGAAERSPAGQLTHLLYLHGFRSSPQ
                     SAKARWMAEWAARHRPDLVWACPQLPPSPAAALADIRAIVAGWPAERTGVIGSSLGGF
                     YATIVAEMLACRAVLLNPAVDPARDLAAHIGRQTMYHAQDETFDFRAEYVDELRAMAP
                     PARLAHPENILAIIAKGDEVLDWREMTARYAHGPIKLIAGSDHGLTDFESHAPDLLRH
                     LGL"
     gene            complement(5148..6521)
                     /gene="mpl"
                     /locus_tag="VIN75_06265"
     CDS             complement(5148..6521)
                     /gene="mpl"
                     /locus_tag="VIN75_06265"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY8973804.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHKVTGCDANVYPPMSDQLRA
                     LGIDLIEGYGTDQVAIAPDLFVVGNAVSRGNPLMEAILDEGLPYASGPQWLAEHVLEG
                     RHVLAVAGTHGKTTTTAMLAWILEQAGLEPGFLVGGVPRNFGVSARLGKARTAGGARP
                     LFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTL
                     PAHGRIVVNAREESLQRVLERGCWSEVQRFGARKEEPGALRARGEPHAFDVLRGSMKI
                     AHVEWHLLGEHNQMNALAAIAAAEHVGVAPEVAAKALASFENVARRLELKGEAGGVKV
                     YDDFAHHPTAIRTTIDGLRRKVGAQRILAVFEPRSNTMKQGAMKAQLPWALEEADLSF
                     CHSGGLSWDAGEALAPLGEQARVCDTIGALVAAVAKAAKPGDHVLCMSNGGFGGIHAK
                     LLDALEK"
     gene            complement(6629..7471)
                     /locus_tag="VIN75_06270"
     CDS             complement(6629..7471)
                     /locus_tag="VIN75_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEY8973805.1"
                     /translation="MATTRPTEYMGHPAVALTSDDGARATVLLHGGHLVSWIPAGGQE
                     QLYVSPTSRYGEGEAVRGGVPVIFPQFSNRGTLPRHGLLRTRGWKLVEMPSHGKHAQA
                     VLRFVADDATRQLWPHEFEAEMTFSLMGPQLDIEFLVVNNGDAPFEFTVALHTYLRTN
                     DVLKAQLEGLQGVKYEDNTTGQWQQQWGDVTQVVGEIDRLYHDAPGELMLREMGRKLA
                     IAHADFTDVVVWNPGPDKAAQLNDLPDDDWTKMLCVEAARVIDPVTLPPGEEWAGMQT
                     LIAN"
     gene            complement(7480..8778)
                     /gene="hemL"
                     /locus_tag="VIN75_06275"
     CDS             complement(7480..8778)
                     /gene="hemL"
                     /locus_tag="VIN75_06275"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8973806.1"
                     /translation="MSTNEQLFERAQKVIPGGVNSPVRAFRAVGGTPRFIARAEGAYV
                     VDAEGKRYVDYIGSWGPMILGHGHPAVLDAVLKAARDGFSFGAPTEREVELAEEIIRH
                     VPSVEQVRLVSSGTEACMSAIRLARAATGRSRLVKFEGCYHGHADALLVKAGSGLATF
                     GHPTSAGVPPEVVQHRTVLDYNSIEQLEQAFAAHGSELACVIIEPIAGNMNFVRSGVP
                     FMKRLRELCSQHGALLIMDEVMTGFRVALGGAQSLYAQQIPGFAPDISVFGKVIGGGM
                     PLAAFGASRRIMAQLAPLGPAYQAGTLSGNPVATACGLATLHEIARPGFHAALGARTR
                     EVMAALKSEADAAGIPLTVDSEGGLFGFFFADRLPHDYTTAVAVDKERFNRFFHAMLD
                     GGVYLAPALYEAGFVSAAHGPAEIEHTRLAARAAFAALNA"
     gene            complement(8768..9721)
                     /locus_tag="VIN75_06280"
     CDS             complement(8768..9721)
                     /locus_tag="VIN75_06280"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY8973807.1"
                     /translation="MSNDPHSGAETPDEIAEEASAPACILTFNASDASGAGGVAGDVA
                     TIAAMGAHALPVVTSVLMRDTAEIFDHHVIDADAVVEQARCILEDITVSGFKVGYLGS
                     TDNVSAVAEVLSDYPEVPLVSYLPALSFMEDDDQATYLEAFRELILPATEVLVGNHKT
                     LTDFLLPEWDSDRPASPRELAVAAAEHGTRFVLVTGVMLPAKGAGQFIDNVLASPQGA
                     LTGEKFEMFEATFVGAGDTLSAAMAALLGAAAELQAAVGEALQFLDQSLDAGFRPGMG
                     GVVPDRFFWALPPGEDEDGEQTVEDALSDDSTEVGGSRSVH"
     gene            9872..10024
                     /locus_tag="VIN75_06285"
     CDS             9872..10024
                     /locus_tag="VIN75_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY8973808.1"
                     /translation="MCLICGWIYDEAAGDAETGLAPGTAWAEVPMNWTCPECGARKED
                     FEMVRI"
     gene            10064..10486
                     /locus_tag="VIN75_06290"
     CDS             10064..10486
                     /locus_tag="VIN75_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973809.1"
                     /translation="MADATPASTAAPASPSVRVLVIDDSNTIRRSAEIFLRQGGHEVV
                     LAEDGFDALAKVNDHTPDLIFCDILMPRLDGYQTCAIIKRNPRFAAVPVIMLSSKDGV
                     FDKARGRMVGSEDYLTKPFTKEQLLRAVAQYNPARSEQ"
     gene            10579..10944
                     /locus_tag="VIN75_06295"
     CDS             10579..10944
                     /locus_tag="VIN75_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973810.1"
                     /translation="MPIQHILLVDDSKTELAFLSDLLAKRGFTVRTAENGEDAMKRLA
                     EGKPDLILMDVVMPGTNGYQLTRAITRDPRYADVPVIMCTSKGQETDRVWGLRQGARD
                     YVVKPVDTDDLMAKIKSLG"
     gene            10985..11524
                     /locus_tag="VIN75_06300"
     CDS             10985..11524
                     /locus_tag="VIN75_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8973811.1"
                     /translation="MANKDALRELQTRLASRLQAAKTQPRARGWLAVEVAGVGLLLPL
                     AQAGEIFPLRALLKLPHAKPWMTGVAQLRGDLYTVIDMAAFLGLRAPKSSSDPSALDG
                     QLVMMAPSLQVNAALRVDRLAGLRGEEQMTREAEPAESVSARPAFAAGRWKDAQGASW
                     QEIDLGALAVDGAFLDVMS"
     gene            11639..>12918
                     /locus_tag="VIN75_06305"
     CDS             11639..>12918
                     /locus_tag="VIN75_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEY8973812.1"
                     /translation="MNLSDQSSGNALKQHGAHTQGSVDNVYPPVASEHGRDVALADVD
                     LGDPAEAHAHSLHDSADGTHAEHRTHALGTGGGVPTLDLDVHAMGHSAFPLDGAGPGQ
                     DDDHVAHSENNLSIDLPLEGVDTAHANSTLVPDQAEPAPTRLQDDGEPEQEVAAIDED
                     DTGLPMIGRRSIGYQQRFVITLAAIGFVGLLVGGYGAISAANAHAKQVEATGAALMQS
                     QRLAKSVSQAMIGSPAAFPEVKESGTVLATDVRGLHSGNDVVTAVPGAAQDTVQPLLA
                     LVDRAEKNSGIVLAQQKTLTQVGQALRTINRQSSDLLETAETVSSLKLQGGASAAEIS
                     AVGQLVMLTQRIGKSANEFLTYEGVSPEAVFLLGKDLNTFREIADGLLQGNADLRLPG
                     TKEPATRERLEQLIQQYEQTRSQSSAILANLQGLV"
BASE COUNT         1890 a         4450 c         4602 g         1976 t
ORIGIN      
        1 gtggcggcgc cttcggcggg cggggtctct tcggtgacgt cgatggccag cgtgagcggg
       61 ccggcgctcg cctcctcggc atcgtcggcg tcctcggcgg cggcctcgtc ggtggcggcc
      121 tcgtcgtcca ggcgcgcgac caggcgggca tgcacgtcga gcgtgagcag gtcgacgcca
      181 tccacgcgca cccgcacgtg cgtgccgcgc ggcaggctgt cgcagcctgc ggccttgaag
      241 accagcggca gcgtgttcgc ccgcacgagg ccgtccttga gcaccgcggc gtccagctcg
      301 gtcacgccgt tctgctgcag ccagcgcagc gtccagaagc gctcgatgcc ggtctggaag
      361 ccgttgtagg ccgagtacgc ggtgtcgaag cccgagatga tggagaacag cgtcgcgtcc
      421 ttgggcttga acggcgccga cagcggcgcc atgcggccgt tgcgcacgca cgcgatgatc
      481 tgccactggt tgacgaggtc gacgtagcgc cgcagcggcg acgtcgccca ggtgtactgc
      541 gccacgccca tgccggcgtg cggcgcgggc ttgatgccca tgcgcacctt gacgcccggc
      601 gcgaggctgg cctggctgcg gtagatgccc ggcacgccca tctcggcgat ccagccgccc
      661 caggtgctgt tggccaggat catggcctcc gagacgatga ggtccagcgg cgcgccacgg
      721 cggcgttcgg tgatggtgac cgtctcgtcg ccggcgggct cgctggtggg cgaccggccg
      781 tcggcggcct gcaggcggaa gttgtagtcg ggccggttga agttctcggg cttgccgcgc
      841 accacctcgc gcagcgcctt gaggtggcgc gcgaggcgga acgtgaacgc gagctcggtg
      901 gcgaacgcgt agtcggccgg cgcctcgccc agcagggact gctcggtgac gacggcatcg
      961 agcttgtcgt ggcgcaggtt ggccacgatg ggcacgcgct cgaccctggt ctcgctgccc
     1021 ttgacctcca gcgtggcctc gtcgaaggtg acgtacaggc tcagcgccgg gcagtcgcgg
     1081 ccttcggcga gcgtgtactt ctcgacgatg gcgtcgggca gcatcgtgat cttccagccc
     1141 ggcatgtaga ccgtggacag gcgttcgcgc gccaccttgt cgacggggct gtccggcagg
     1201 atcgccaggc ccggcgcggc gatgtgcacg ccgaagacga cggtgccggt gcccaggccg
     1261 cggacggaca gcgcgtcgtc gatctcggtg gtctgggagt cgtcgatcga gaacgcggcc
     1321 acctcggcca cgggcaagtc gtccttgacc ggtggcgcct cgagcggcgg gaagcccgtg
     1381 cccttgccga actcctcgaa caggaagcgg cgccagtgga actggtaggg gctcgtgatg
     1441 gcgcccgcgg ccttgagcag gtcgagcggc gagcgctgcg actgcttgga cgcctcgacg
     1501 acggccttgt actcgggcgc gttcttgtcg ggacggaaca ggatcttgta gagctggtcg
     1561 cggatcggct gcggcgtcgt gccggccacc agttcgccgg cccattcctg gatctgcagc
     1621 gcctgcaggc gcttgcgctc gatgcccagc agcgcggcct tgaccgtctc ctcgggcgcc
     1681 ttgcggaaca cgcccttggc catgcggcgg aagtagtgcg gcgcctcgaa caggcggaac
     1741 agggcggcgg cctgctgcac ggggccggcc gaggcgtcga agtagtcgcg cgccaggtcg
     1801 gcgaagccga attcggcctc gctggcgaat tcccaggcga ggtcgaggtc gatctcgcgc
     1861 gcgagcgcct ggccctgcgc gatcagttcc gcgggctgcg gcttctcgaa gcgcagcagc
     1921 acatgcgaag acttgacctt cacacgcttg ccggactcga gttccacctg gatcgagctg
     1981 tcggcctcgg acatcacccg tccggcgagg aacttgccgg cgtcatcgaa gagagcgtaa
     2041 ttcacccggc gattgtcgcc gagttcgggc accgggccgg gctgggcagt gttgcgggag
     2101 gtttcatttc cggccttcgc agggcgggtc agccgcaggc gtgacgcgtc atgtcgacag
     2161 ggcgcacgcc ggtgaacggc gggtggcgcc tgcggctgac ccgccctacg ggtcgccgat
     2221 accgcgatgt cgccgcttca ggccgcagtc tgtctcccgg attcgagcgc gaatccttcg
     2281 tccagccagc cggtcacgcc gccgatcatg atcttgaccg gccgccccag accagccagc
     2341 cggatcgccc cgcgggtcgc gccgttgcag tgcggtccgg cgcagtaggt gacgaacagc
     2401 gtgccctcgg ggaactcgcg catcttgcct tcggtgatct tgccgtgcgg caggtgcagc
     2461 gcgcccggca cgtggccggc cgcgtacgtc tcggcgctgc gcacgtccag cagcacgaag
     2521 tcgcgctggc cggtcgcgag gctgtggtgg acgtcccagc agtcggtctc gaactgcagg
     2581 ctggcttcga agtgcggcag cgcggcggag gacggggcgg cggggatggt ggcgacgggc
     2641 gaggtcatga gggctccttg gtgcgtgtgg cctcattctc gaagtggcgg cccgtccgtt
     2701 gaagtggcgc gaaagacaac taccggtaag atcgcgccat gaagaaccat cttgtcgccg
     2761 cgctcgccta cgacggcctg tgcaccttcg agttcggctg cgccgtggag gtgttctcgc
     2821 tcgagcggcc cgagctgggc gtcgactggt accgcttcgc ggcctgcgcc gagtcgcgcg
     2881 gcacggtgcg cgcggccggc ggcatctccg tgcaggtgcc gcacggcctc gcgacgctcg
     2941 cgcgcgccgg caccatcgtg atccccggct ggcagggggt ggacgtcgcg ccgtcgcccg
     3001 tgctgctgcg acggctcgtc gcggcgcacg agcgcggcgc gcgcatcgcc accatctgct
     3061 cgggcgtgtt cgtgctcgcc gccacgggcc tgctcgatgg caaggccgcc accacgcact
     3121 ggcgctacgc ggacctcctg cagcagcgcc atccgcgcat cgacgtgcgt ccgaattcga
     3181 tctacgtcga cgagggcccg ctgctgacgt cggccggctc cgccgcgggc ctggacatga
     3241 tgctgcacct cgtgcgccgc gaccacggtc cgcgcgtggc caaccaggtc gcgcagcgcc
     3301 tggtcgtgcc gccgcaccgc ctcggcgacc aggcgcagtt cgtcgcgcgc ccgctgccgc
     3361 aggacgaggc cgcgcgcctg gcacgcctga tggacttcgt gcgcgcgcac ccggcgcagg
     3421 actggacgct tgcctcgctc gccgatcgcg cggcgatgag cgtgcgcacg ctgcagcgcc
     3481 agttccagca gtcgctgggc atgtcgccgg tcgactggct cgtgcgcgaa cgcgtcgagg
     3541 tcgcgcgcca gctgctcgag acgagcacgc ggccgctggc gcatgtctcg accctggccg
     3601 gcttcggctc cgaggaatcg ttccgccgcc acttccggct ggccacgggc gtcagtccgc
     3661 tggcctatcg gcggcagttc ggcgtcgcgg cgtgaaggtc agacggtcat cccgcccgac
     3721 acctcgatga cggcgccgtt gacgtagctc gcctcgtcgc tcgcgaggaa ggcgtagaca
     3781 ttggcgatct cctcgggctt gcccaggcgg tgcagcggca cctgctcgcg catgtgctgc
     3841 agcaccttgt cgggcacggt cgcgaggatg ggcgtctcga cgaatcccgg cgccaccgcg
     3901 ttgacgcgca cccccttggg cccgagctcg cggctccagg tcttggtgaa gccgatcacg
     3961 ccgaacttcg tggcggcgta gttggtctgg ccgaagttgc cgtagaggcc gaccaccgaa
     4021 ctcgcgttga ggatgctgcc cgcgccctgc gccaccatcg tgtcggccac ggcctgcgtg
     4081 caatggaaca cgccgcgcag gttgacgtcg atcaccgcgt cgaactgctc gatcgtcatc
     4141 ttctgcaggc gtgcgtcccg ggtgatgccc gcgttgttga cgagcacgtc gatgcggccg
     4201 aagcgcgcct tcacggccgc gaccatcgcg tcgacctgcg ctcggtcggt gacgtccacc
     4261 gcctggccga tgcaggccgc gcctgccacg gacacggcgt tgaccgcggc gacggcggca
     4321 tcgacgtcgt gcacgtcgca gacgacgacg ctggcgcctt cgcgggcgaa cttcagggcg
     4381 gtggccaggc cgatgccctg ggcggcgccg gtgacgatgg cgatcttgtc ggacagacgc
     4441 atgacggaac gcggggttga ggtgcgtcga ttgtccccga cgccgtcaga gtccgaggtg
     4501 gcgcagcagg tccggcgcgt gcgactcgaa gtcggtcagg ccgtggtcgc tgcccgcgat
     4561 cagcttgatc ggcccgtggg cgtagcgcgc ggtcatctcg cgccagtcca gcacctcgtc
     4621 gcccttggcg atgatcgcga ggatgttttc cggatgcgcg agacgcgccg gcggcgccat
     4681 cgcgcgcagc tcgtcgacgt actcggcgcg gaagtcgaag gtctcgtcct gcgcgtggta
     4741 catcgtctgg cggccgatgt gggccgccag gtcgcgtgcc ggatcgacgg ccgggttcag
     4801 cagcaccgcg cggcaggcga gcatctcggc gacgatggtg gcgtagaagc cgcccagcga
     4861 gctgccgatg acgccggtgc gctccgcggg ccagccggcg acgatggcgc ggatgtcggc
     4921 gagcgccgcg gcgggcgagg gcggcagctg cgggcaggcc cacacgaggt cgggccggtg
     4981 cctggcggcc cattcggcca tccagcgcgc cttggccgat tgcggcgagg agcggaagcc
     5041 gtgcaggtac agcaggtgcg tcagttgtcc ggccggcgag cgctcggccg ctcccgagcg
     5101 agccggcctg agcccctcgg gggcggggag cggagcgagc cgggccgtca tttctccagc
     5161 gcgtccagca gcttggcgtg gatgccgccg aagccgccgt tgctcatgca cagcacatgg
     5221 tcgccgggct tggccgcctt ggcgacggcg gccaccagcg cgccgatggt gtcgcacacc
     5281 ctggcctgct cgcccagcgg cgccagcgcc tccccggcgt cccacgacag gccgcccgag
     5341 tggcagaacg acaggtcggc ctcctccagc gcccacggca gctgcgcctt catcgcgcct
     5401 tgcttcatcg tgttggagcg cggctcgaac accgccagga tgcgttgcgc gcccaccttg
     5461 cggcgcaggc cgtcgatggt cgtgcggatc gccgtgggat ggtgcgcgaa gtcgtcgtag
     5521 accttcacgc cgcccgcctc gcccttgagt tcgagccggc gcgccacgtt ctcgaacgac
     5581 gcgagcgcct tggccgcgac ctcgggcgcg acgcccacgt gttcggcggc ggcgatcgcg
     5641 gccagcgcgt tcatctggtt gtgctcgccc agcaggtgcc attcgacgtg ggcgatcttc
     5701 atcgagccgc gcagcacgtc gaacgcgtgc ggctcgccgc gggcgcgcag cgcgccgggc
     5761 tcttccttgc gcgcgccgaa gcgctgcacc tcgctccagc agccgcgctc gagcacgcgc
     5821 tgcagcgatt cctcgcgcgc gttgacgacg atgcggccgt gcgcgggcag cgtgcgcacg
     5881 aggtggtgga actgggtctc gatggcggcc aggtcgggga agatgtccgc gtggtcgaac
     5941 tcgaggttgt tcagcaccgc ggtgcgcggc cggtagtgca cgaacttgct gcgcttgtcg
     6001 aagaacgcgg tgtcgtattc gtcggcctcg atgacgaaca gcggccgcgc gccgccggcc
     6061 gtgcgcgcct tgcccagccg cgccgagacg ccgaagttgc gcggcacgcc gcccaccagg
     6121 aagcccggtt ccagtccggc ctgctcgagg atccacgcga gcatcgcggt ggtcgtcgtc
     6181 ttgccgtgcg tgccggccac ggccagcacg tggcggcctt ccagcacgtg ctctgccagc
     6241 cattgcgggc cgctggcgta cggcaggcct tcgtcgagga tcgcctccat cagcgggttg
     6301 ccgcgcgaga ccgcgttgcc gacgacgaac aggtcgggcg cgatcgccac ctggtcggtg
     6361 ccgtagccct cgatcaggtc gatgccgagc gcacgcagct ggtcgctcat cggcgggtag
     6421 acgttggcgt cgcagccggt gaccttgtgg ccggcctcac gtgccagtgc cgcgaggcca
     6481 cccatgaagg tgccgcagat gcccaggatg tggatgtgca tgacgattcg ttcttgttgc
     6541 cgtcccgcgt cggccatgca cgtttcgcgc ccggccgccg ggatctcggc cgcgggcggc
     6601 tgcgccgccg cggtcttgtc gataggaatc agttcgcgat cagagtctgc atcccggccc
     6661 attcctcgcc cggcggcagg gtgaccggat cgatgacgcg tgccgcctcc acgcacagca
     6721 tcttcgtcca gtcgtcgtcg ggcaggtcgt tcagctgggc ggccttgtcg gggccggggt
     6781 tccagacgac gacgtcggtg aagtcggcgt gcgcgatggc cagcttgcgg cccatctcgc
     6841 gcagcatcag ctcgcccggc gcgtcgtggt acaggcggtc gatctcgccg accacctgcg
     6901 tgacgtcgcc ccattgctgc tgccactggc cagtggtgtt gtcctcgtac ttcacgccct
     6961 gcagcccctc gagctgggcc ttgagcacgt cgttggtgcg caggtaggta tgcagcgcga
     7021 cggtgaactc gaacggtgcg tcgccgttgt tgacgaccag gaactcgatg tccagctgcg
     7081 ggcccatcag gctgaacgtc atctcggcct cgaactcgtg cggccacagc tggcgcgtgg
     7141 catcgtcggc gacgaagcgc agcaccgcct gggcgtgctt gccgtgcgag ggcatctcca
     7201 ccagcttcca gccgcgcgtg cgcagcaggc cgtggcgggg cagggtgccc cggttggaga
     7261 actgcgggaa gatcaccggc acgccgccgc gcacggcctc gccctcgccg tagcggctgg
     7321 tgggcgagac gtacagctgc tcctggccgc cggccgggat ccagctgacc aggtggccgc
     7381 cgtgcagcag gacggtggcg cgcgcgccgt cgtcggaggt gagcgccacg gcagggtggc
     7441 ccatgtattc ggtgggacgg gtcgtggcca cgggggatgt caggcgttga gtgcggcgaa
     7501 ggcggcgcgg gcggccaggc gcgtgtgttc gatctcggcg gggccgtgcg cggcgctgac
     7561 gaagccggcc tcgtacagcg cgggcgccag gtagacgccg ccgtccagca tcgcgtggaa
     7621 gaagcggttg aagcgctcct tgtcgacggc cacggcggtc gtgtagtcgt gcggcaggcg
     7681 atcggcgaag aagaagccga acaggccgcc ctcgctgtcg accgtgagcg gaatgccggc
     7741 ggcatcggcc tcggacttca acgccgccat cacctcgcgg gtgcgggcgc cgagcgcggc
     7801 gtggaagccc ggcctggcga tctcgtgcag cgtggccagg ccgcaggccg tggcgaccgg
     7861 attgccggac aacgtgcctg cctggtaggc cgggccgagg ggcgccagct gcgccatgat
     7921 cctgcgcgac gcgccgaacg ccgccagcgg catgccgccg ccgatcacct tgccgaacac
     7981 gctgatgtcg ggcgcgaagc cggggatctg ctgcgcatag aggctctgcg cgccaccgag
     8041 cgcgacgcga aagcccgtca tcacctcgtc catgatcaac agcgcgccgt gctggctgca
     8101 cagctcgcgc aggcgcttca tgaacggcac gccggagcgc acgaagttca tgttgccggc
     8161 gatgggctcg atgatcacgc aggccagctc gctgccgtgc gcggcgaacg cctgctccag
     8221 ctgctcgatg ctgttgtagt cgagcacggt cctgtgctgc accacctcgg gcggcacgcc
     8281 ggccgacgtc ggatggccga aggtggccag gcccgagccg gccttgacca gcagcgcgtc
     8341 ggcgtggccg tggtagcagc cctcgaactt gaccaggcgc gagcgcccgg tggcggcgcg
     8401 cgccagccgg atcgcgctca tgcaggcctc ggtccccgag ctgaccaggc gcacctgctc
     8461 gacgctgggc acgtggcgga tgatctcctc ggccagctcg acctcgcgct cggtgggcgc
     8521 gccgaagctg aagccgtcgc gcgccgcctt cagcacggca tcgagcacgg cgggatgccc
     8581 gtggcccagg atcatcggtc cccacgagcc gatgtagtcg acatagcgct tgccctcggc
     8641 atccaccacg tacgcgccct cggcgcgcgc gatgaatcgc ggcgtgccgc cgaccgcgcg
     8701 gaaggcgcgc acgggcgagt tcacgccgcc ggggatgacc ttctgggcgc gctcgaacag
     8761 ctgctcatta gtggacactg cgcgagcctc cgacttcggt cgaatcgtcc gacagcgcgt
     8821 cttccacggt ctgctcgccg tcctcgtcct cgccgggcgg cagcgcccag aagaagcggt
     8881 cgggcaccac gccgcccatg ccggggcgga agcccgcgtc caggctctgg tcgaggaact
     8941 gcagcgcctc gccgacggcg gcctgcagct cggcggccgc gcccagcagc gcggccatcg
     9001 cggccgacag ggtgtcgccg gcgcccacga aggtggcctc gaacatctcg aacttctcgc
     9061 cggtgagcgc gccctggggc gaggccagca cgttgtcgat gaactggccg gcgcccttgg
     9121 ccgggagcat cacgcccgtc accaggacga agcgggtgcc gtgctcggcc gcggccaccg
     9181 ccagctcgcg cggcgaggca gggcggtcgg aatcccattc gggcagcagg aagtcggtca
     9241 gcgtcttgtg gttgcccacc agcacctcgg tggccggcag gatcagctcg cggaaggctt
     9301 cgaggtaggt cgcctggtcg tcgtcctcca tgaaggacag cgccggcagg tacgagacca
     9361 gcggcacctc gggataatcc gacagtactt cggcgacggc actgacgttg tcggtactgc
     9421 cgagatatcc caccttgaag cccgagaccg tgatgtcctc gaggatgcag cgcgcctgct
     9481 cgaccacggc gtcggcgtcg atcacgtggt ggtcgaagat ctcggcggtg tcgcgcatga
     9541 gcacggaggt gaccacgggc agggcgtggg cgcccatcgc ggcgatggtc gcgacgtcgc
     9601 ccgccacgcc tcccgcgccg ctggcatcgc tcgcgttgaa cgtcaggatg caggcgggcg
     9661 cgctggcttc ctcggcgatt tcgtcgggcg tttccgcgcc ggaatggggg tcgtttgaca
     9721 tggccggtgc cggtcaagtt gtgaagcttg cccggaaaat gtgattagtt tggcccttaa
     9781 cgcgtcgtta accgctcggg gccgtggcta caatccagac gattgtagtg accctgaacg
     9841 cgaaacgact gtgaacgaac ctcaaacctg gatgtgcctg atctgcggct ggatctacga
     9901 cgaagctgcc ggcgacgctg aaactggttt ggcccccggc accgcctggg ccgaggttcc
     9961 gatgaactgg acgtgtccgg aatgcggggc acgcaaggaa gatttcgaaa tggtccgcat
    10021 ctgaccgatg agcaccagat ggatcaactg aacgggattt tcaatggctg atgccacgcc
    10081 ggcttccacc gccgcccccg cgtcgccctc ggtgcgcgtg ctcgtgatcg acgacagcaa
    10141 caccatccgg cgcagcgccg agatcttcct gcgccaggga gggcatgaag tggtgctggc
    10201 cgaagacggt ttcgacgcgc tggccaaggt caacgaccac acgcccgacc tgatcttctg
    10261 cgacatcctg atgccgcggc tggacggcta ccagacctgc gcgatcatca agcgcaaccc
    10321 gcgcttcgcc gcggtgccgg tgatcatgct gtcgtccaag gacggcgttt tcgacaaggc
    10381 gcgcgggcgc atggtcgggt cggaggatta cctgaccaag ccgttcacga aggagcagct
    10441 cctgcgtgcg gtggcccaat acaacccggc ccgatctgaa cagtaagagt ttctccgcac
    10501 ggatattccc ggccgcgacg agcgaacgga agtaaacgag caaaagcgca taccaaggcg
    10561 cgtttccaag gacacaccat gccgatccag cacattctcc tggtcgatga ttcgaagacc
    10621 gaattggctt ttctctccga cctgctcgcc aagcgcggtt tcaccgtgcg caccgccgag
    10681 aatggcgaag acgcgatgaa gcgcctggcc gaaggcaagc ccgacctgat cctgatggac
    10741 gtcgtcatgc ccggcaccaa cggctaccag ctgacccgcg cgatcacccg cgacccgcgc
    10801 tacgccgacg tgcccgtgat catgtgcacc agcaaggggc aggagaccga ccgcgtctgg
    10861 ggcctgcgcc agggcgcccg cgactacgtc gtcaagcccg tcgacaccga cgacctgatg
    10921 gccaagatca agtccctcgg ctgaggccag gcgatctgac cccgaccacc tcgcacgcac
    10981 ccccatggcc aacaaagatg ccctgcgcga actgcaaacc cggctggcaa gccgtctgca
    11041 agccgccaag actcagccgc gtgcgcgagg ctggctcgcc gtcgaagtcg ccggcgtcgg
    11101 cctgctgctg ccgctggccc aggccggcga gatcttcccg ctgcgggcgc tgctgaagct
    11161 cccgcacgcc aagccctgga tgaccggggt ggcgcagctg cggggcgacc tgtacaccgt
    11221 gatcgacatg gcggccttcc tgggcctgcg cgcgcccaag tcctccagcg atccgtcggc
    11281 gctcgatggc cagctggtga tgatggcccc gtcgctgcag gtcaatgcgg cactgcgcgt
    11341 cgaccgcctg gccggcctgc ggggcgagga acagatgacg cgcgaggccg agccggccga
    11401 gagcgtgagt gcccggcccg cgttcgcggc cggtcgctgg aaggatgcgc agggcgcgtc
    11461 ctggcaggaa atcgatctgg gagcgctggc cgtcgacggc gcgttcctgg atgtgatgag
    11521 ctgaacagag ggttcggcag cgacgacgcg ccgcgcgagc ggcgccggca ggccagcaag
    11581 gcgtcgggca gccgttcggg attagttggg atttgaaatc tagcgtgaag agggcgagat
    11641 gaacttgtca gaccaaagca gcggtaacgc gctcaagcaa cacggcgccc atacccaggg
    11701 cagtgtcgat aacgtctatc cgcccgtcgc ttccgagcac ggccgcgacg tggcgctggc
    11761 cgacgtcgac ctgggcgatc cggcggaggc gcacgcgcac tcgctgcacg actcggccga
    11821 cggcacccac gcggagcacc ggacgcacgc gctgggcacc ggcggcggcg tgcccacgct
    11881 ggacctggac gtccacgcga tgggccactc ggccttcccg ctggacggcg ccggccccgg
    11941 ccaggacgac gaccacgtcg cccactccga gaacaacctg tcgatcgacc tgccgctgga
    12001 aggcgtcgac accgcccacg cgaactccac gctggtgccc gaccaggccg agccggcgcc
    12061 cacgcgcctg caggacgacg gcgagcccga gcaggaagtc gccgcgatcg acgaggacga
    12121 caccggcctg ccgatgatcg gccgccgctc gatcggctac cagcagcgct tcgtgatcac
    12181 gctggcggcc atcggcttcg tcggcctgct ggtcggcggc tacggcgcca tctcggcggc
    12241 caatgcccac gcgaagcagg tggaagccac cggtgcggcg ctgatgcagt cgcagcgtct
    12301 cgcgaagtcg gtctcgcagg ccatgatcgg ttcgccggcg gccttcccgg aagtgaagga
    12361 aagcggcacg gtgctggcca ccgacgtgcg cggcctgcat agcggcaacg acgtcgtcac
    12421 cgccgtgccc ggcgccgcgc aggacaccgt ccagccgctg ctggcgctgg tcgatcgcgc
    12481 cgagaagaac tccggcatcg tgctggcgca gcagaagacg ctgacccagg tcggccaggc
    12541 gctgcgcacc atcaatcgcc agtcgtccga cctgctcgag accgcggaaa ccgtgtcgtc
    12601 cctgaagctg cagggcggtg cctcggcggc cgagatctcc gccgtcggcc agctcgtcat
    12661 gttgacgcag cgtatcggca agagcgcgaa cgaattcctc acctacgaag gcgtgtcgcc
    12721 cgaagccgtg ttcctgctcg gcaaggactt gaacaccttc cgcgagatcg ccgacggcct
    12781 gctgcagggc aacgccgacc tgcggctgcc gggcacgaag gagcccgcca cgcgcgagcg
    12841 cctcgagcag ctgatccagc agtacgagca gacgcgcagc cagtcgagcg cgattctggc
    12901 caacctgcag ggcctggt
//
LOCUS       DATCRO010000232         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_868608, whole genome shotgun sequence.
ACCESSION   DATCRO010000232 DATCRO010000000
VERSION     DATCRO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868608"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="dnaA"
                     /locus_tag="VIN75_06310"
     CDS             complement(<1..1287)
                     /gene="dnaA"
                     /locus_tag="VIN75_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8973813.1"
                     /translation="MSVDLWHRSCERLATELPEQQFSTWIRPLPPADFTDVGDGAVAL
                     VRVPNRFKLDWIRSQYGSRIESVLTELAGKPVKLELALAPRETPAPGHAAVSSGGGFI
                     TATPPQRLGTVFNQALPATAAHDGASNGNAGVAIAPAQPRPMHAAPAMNSHRLNPALT
                     FDTLVAGRANQMARTAALHVAGAPGAMYNPLFVYGGVGLGKTHLVHAVGNQLLKDKPD
                     ARVLYLHAEQFISDVVKNYQRKTFDELKAKYHNLDLLLIDDVQFFAGKERTQEEFFNA
                     FEALLSRRAHIIMTSDTYPKGLADIDERLTSRFDSGLTVAIEPPELEMRVAILIRKAE
                     AEAAQMPEEVAFFIAKNVRANVRELEGALRKVLAYSRFSQKEINIALAREALKDLLSI
                     QNRQISVENIQKTVADFYKIKVADMYSKKRPASIARP"
     gene            1468..1602
                     /gene="rpmH"
                     /locus_tag="VIN75_06315"
     CDS             1468..1602
                     /gene="rpmH"
                     /locus_tag="VIN75_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8973814.1"
                     /translation="MKRTYQPSKVRRARTHGFLVRMKTRGGRNVIAARRAKGRKRLAV
                     "
     gene            1751..2440
                     /locus_tag="VIN75_06320"
     CDS             1751..2440
                     /locus_tag="VIN75_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY8973815.1"
                     /translation="MTAADLDSPLLGRIVRPADYVRVLATPMRLRSPHFAVHHLAGRP
                     LPLRRPSPGEVAGSGNAEARDRGHAPVTASLSTELSTGGGDAALGDVDDSHVQCPQPL
                     VETPTPAGGIDRWLGLVVPKRHARRAVTRTLMKRQIRHIAAACASQLEPGLWVVRQRN
                     PFDVKQFPSAASDALKEAARAELRALFERAVRGERDRIKPRPPGAVEKRPGTKSRTAP
                     GASAQGPACAA"
     gene            2428..2802
                     /gene="yidD"
                     /locus_tag="VIN75_06325"
     CDS             2428..2802
                     /gene="yidD"
                     /locus_tag="VIN75_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8973816.1"
                     /translation="MRGLNALWRLPRAALIGLVRVYRLLLSPWVGQSCRFTPTCSAYA
                     MQALDKHGAIVGTGLAGWRILRCNPWCHGGCDEVPDNMPWNRTRATPSAGLFSALLDR
                     DSGSAPPATTAADKTNLSNTPT"
BASE COUNT          424 a         1005 c         1000 g          482 t
ORIGIN      
        1 cgggcgggcg atgctcgccg ggcgcttctt cgagtacatg tcggccacct tgatcttgta
       61 gaagtcggcg acggtcttct ggatgttctc caccgagatc tgccggttct ggatcgagag
      121 caggtccttg agcgcctcgc gtgccagcgc gatgttgatc tccttctggg agaagcgcga
      181 ataggccagc accttgcgca gcgcgccctc gagctcgcgg acgttggcgc gcacgttctt
      241 ggcgatgaag aacgccactt cctcgggcat ctgggcggct tcggcctcgg ccttgcggat
      301 caggatggcc acgcgcatct cgagctcggg cggctcgatg gccaccgtca ggcccgagtc
      361 gaagcggctc gtcaggcgtt cgtcgatgtc ggcgagaccc ttgggatagg tgtcgctcgt
      421 catgatgatg tgcgcgcggc gcgagagcag cgcctcgaag gcgttgaaga actcctcctg
      481 cgtgcgctcc ttgccggcga agaactggac gtcgtcgatc agcagcagat cgaggttgtg
      541 gtacttggcc ttgagctcgt cgaaggtctt gcgctggtag ttcttgacga cgtcggagat
      601 gaactgctcg gcgtgcaggt agagcacgcg cgcgtcgggc ttgtccttga gcagctggtt
      661 gccgacggcg tgcaccaggt gggtcttgcc caggccgacg ccgccgtaga cgaacagcgg
      721 gttgtacatg gcgcccggcg cgccggccac gtgcagcgcg gcggtgcggg ccatctggtt
      781 ggcgcggccg gccaccagcg tgtcgaaggt cagcgccgga ttgagccggt gcgagttcat
      841 cgccggggcc gcgtgcatcg gacgcggctg ggcgggtgcg atggcgacgc cagcgttgcc
      901 gttggacgcg ccgtcatggg cggccgtcgc cggcagcgcc tggttgaaca cggtgcccag
      961 gcgctgcggc ggcgtggcgg tgatgaagcc gccgcccgac gacacggcgg cgtggcccgg
     1021 ggccggcgtc tcccgcgggg ccagggccag ctcgagcttg accggcttgc cggcgagttc
     1081 ggtcagcacg gactcgatgc gcgagccgta ctgcgagcgg atccagtcga gcttgaagcg
     1141 gttgggcacg cgcacgagcg cgaccgcgcc gtcacccacg tcggtgaagt cggccggcgg
     1201 cagcggccgg atccaggtgc tgaattgttg ttcgggcagc tcggtcgcga ggcgttcgca
     1261 actccggtgc caaaggtcta cggacattgt ggacaagttc ttctcaagcc ggccgattct
     1321 acccgtgact tcaggcgcga aggcacggtt atccacagaa agacgcacgc ggtcaatccc
     1381 cccgcatcgc acggcggcac gtggcaagtc gttgactcac aacggctttc tctgctctaa
     1441 tcgcgggttc gccggagaat ttccatcatg aagcgcacct accagccctc caaagtccgc
     1501 cgcgcccgca cgcacggctt cctcgtccgc atgaagaccc gtggtggccg caacgtcatc
     1561 gccgcgcgcc gcgccaaggg tcgcaagcgt ctggccgtct gattccagac gccggactga
     1621 acgcttcgcc gatcaggaat tgcgcagcgg cccgcgccgc tgccgatgcc cgggcgcagc
     1681 cctcccccgt gggagcgccg cctcggatcc gcgtcgaatt cccgcttcgc ggcacacccg
     1741 gcccgcggcc atgaccgcag ccgacctcga ctccccactg ctcggccgga tcgtccggcc
     1801 ggcggactac gtgcgcgtgc tcgcgacgcc gatgcgtctg cgcagccccc atttcgccgt
     1861 ccatcacctg gcgggccggc cgctgccatt gcgccgcccg tcgccgggcg aggtggccgg
     1921 gtccgggaat gccgaggccc gcgaccgcgg ccatgcaccg gttaccgcca gcttgtctac
     1981 agagttatcc acaggtggcg gcgatgctgc gcttggcgat gtggacgact cgcacgttca
     2041 gtgcccgcaa ccgctggtgg aaaccccgac gccggccggc ggcatcgacc gctggctggg
     2101 cctggtcgtg cccaagcgcc acgcgcgtcg cgccgtgacg cgcacgttga tgaagcgtca
     2161 gatccgccac atcgccgccg cctgtgcttc gcagctcgag cccggcctgt gggtggtgcg
     2221 ccagcgcaac ccgttcgacg tgaagcagtt cccgagcgcc gcctccgacg ccttgaagga
     2281 agcggcgcgg gcagagctgc gcgcgttgtt cgagcgggcc gtccgcggcg agcgcgatcg
     2341 catcaagccg cgaccgcccg gtgcagtcga gaagcggccc ggcacgaagt ccaggacggc
     2401 gcctggcgcc tccgcgcagg ggcccgcatg cgcggcctga acgcgttgtg gcggctgccg
     2461 cgtgccgcgc tcatcggcct cgtgcgggtc taccgccttc tgctgagccc gtgggtcggc
     2521 cagagctgcc gcttcacgcc gacgtgctcg gcctacgcga tgcaggccct ggacaagcac
     2581 ggcgcgatcg tcggcaccgg cctggccggc tggcgcatcc tgcgttgcaa cccgtggtgt
     2641 cacggcggct gcgacgaagt ccctgacaat atgccttgga accgaaccag ggcgacccca
     2701 tctgcgggcc tgttcagcgc actgctggac cgggattccg gctccgcacc gcccgcgacg
     2761 accgcggccg acaagacaaa cctctccaac acccccacat gaccgaattc cgccgcaccc
     2821 tgctgctggt cgtcctgatg atgtcgttgc tgatgctcta cgacaagtgg cgcatctatc
     2881 acggcgactc gtcgttcctg gcaccgaagc c
//
LOCUS       DATCRO010000233         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_384637, whole genome shotgun sequence.
ACCESSION   DATCRO010000233 DATCRO010000000
VERSION     DATCRO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384637"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3818)
                     /locus_tag="VIN75_06330"
     CDS             complement(<1..3818)
                     /locus_tag="VIN75_06330"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8973817.1"
                     /translation="MINVDTTPPARPDAISGYADNFGAVQNTASTAAFTDDTTPGFKI
                     GSVAAGLTPSLYVDGAKVAATFDPNTGTLMPTFPLAEGAHSISYTLTDAAGNESPRAV
                     DFRLAVDTTPPSALDASQLRVLDHVGPVQGPLAAGAKTDDSKPEYTGTADPAQVTAIN
                     VYDNGNLIGSTAVNADGTWRFTPAVPLALGAHSLVARPVDAAGNIGPATPASTFTLVG
                     DPPAAPAIIGVSDDKGSVTGNVAPDASTDDTTPTLRGTGTVGTVVTVYADGAAVGSTS
                     VASDGTWSVTTSALNGDGTKNLTAQAVDGAGQVSPRTGAYAIVLDTTAPATPAVVTAM
                     DDQGPAQGAITAGGVTDDTTPTFSGSGVKAGDTIRLYDGGALIGSITVATDGAWSITP
                     STPLASGAHRITLTVTDAAGNTSAASAPLEFTVDLADVVVSIEQVVDDQGSIKGNLAP
                     GARTDDTTPTLVGKATAGALVTVKEGATVLGSTTADAGGSWRLTLSAQGEGTHSYTAT
                     AVNAAGKKGEATIDLTIDTTAPEVPKIAGVGDDVGLVQGALASGAYSDDTTPTLGGSG
                     ATAGDLIKIYDNGSFLGSATVKADGSWSFTPTTPLAEGAHRFTATTTDSVGNESAHSG
                     SFTVNVDVTPPTAKAAVGAISTDTGTSADDFVTSDRTLVATITLDSALEQGATVQISM
                     DNGRTWSNATPVGNNNYQFDNTAVSLPDGSYTFLARVVDAAGNATPSSSQVVIIDNSG
                     PTADNAVAITAYTDDVAPNVGDYPSGTSTNDTTPVLKGTVSGLKAGDVVQIFEGAVLL
                     GTATVNGAAWTFALPVLANGTAHTYKAVIADAAGNKGTASADFSLSVATAVPSQSAQI
                     VGFTDDQAPLTGTFGSGAVTNDTSPLLNGTFTGTLNTGDVIVIYRNGARLGAATMTGG
                     SAWTFQDAGLVDNTSYSYAAFVEDAAGNRGGSSAAFTLTIDQRAPSTAPSIDSVTDDV
                     APVTGSIASGGTTNDARPELKGSGAEPNGTVRIYDSGTLIGTATVDDAGKWSFTPEAG
                     KELANGVHSLTASSVDAAGNEGPQSVATAFTVDTIAPSQSTTIVSIKDDQGALQGTVA
                     DGGFTDDVAPQLAGTLSAPLQAGEKVVVLRDGAVIGTATMTDATHWTFDDAGLSDGRT
                     YAYTARVEDAGGNRGAISGSYAITIDTSVPAQKAAISTVFDNVEPVQGDVANGGTTND
                     SQPELRGTITGSLATNEFVAVYRDGVKIGIASISGTSWSYTDTSGLANAASYSYTVRV
                     EDAAGNGGAAS"
BASE COUNT          451 a         1224 c         1475 g          690 t
ORIGIN      
        1 gaagccgcac cgccgttgcc ggcggcgtct tcgacgcgca ccgtgtagct gtagctcgcg
       61 gcgttggcga ggccgctggt gtcggtgtag gaccagctcg tgcccgagat cgacgcgatg
      121 ccgatcttga cgccgtcgcg atagacggcg acgaactcgt tggtcgctag gctgccggtg
      181 atcgtgcccc tgagttccgg ctgcgagtcg ttggtggtgc cgccgttggc gacatcgccc
      241 tgcaccggct cgacgttgtc gaacacggtc gagatcgcgg ccttctgcgc cggcacggag
      301 gtgtcgatgg tgatggcgta gctgcccgag atggcgccgc ggttgccgcc ggcgtcctcg
      361 acgcgtgcgg tgtaggcgta ggtgcggccg tcgctcaggc cggcgtcgtc gaaggtccag
      421 tgggtggcgt cggtcatcgt cgcggtgccg atgactgcgc cgtcgcgcag caccaccact
      481 ttctcgcccg cctgcagcgg cgccgagagc gtgccggcca gctgcggcgc gacgtcgtcg
      541 gtgaagccgc cgtcggccac cgttccctgc agcgcgccct ggtcgtcctt gatcgagacg
      601 atggtcgtcg actgcgacgg cgcgatggtg tcgaccgtga aggccgtcgc aacagactgc
      661 gggccctcgt tgccggccgc atcgacgctg ctcgccgtga ggctgtggac gccgttggcc
      721 agctccttgc cggcctccgg cgtgaagctc cacttgcccg cgtcgtccac cgtcgcggtg
      781 ccgatcagcg tgccgctgtc gtagatcctg acagtgccgt tgggctcggc cccggagccc
      841 ttcagctcgg ggcgggcatc gttggtggtg ccgccggaag cgatgctgcc ggtgacgggc
      901 gcgacgtcgt cggtgacgct gtcgatcgag ggcgccgtgc tgggcgcgcg ctggtcgatg
      961 gtgagggtga aggccgccga ggagccgccg cggttgccgg ctgcgtcttc gacgaatgcg
     1021 gcgtagctgt agctggtgtt gtcgaccaga cccgcgtcct ggaaggtcca ggcgctgccg
     1081 ccggtcatgg tggcggcacc gaggcgcgcg ccgttgcgat agatgacgat gacgtcgccc
     1141 gtgttgagcg tgccggtgaa ggtgccgttg agcagcggcg aggtgtcgtt ggtgacggcg
     1201 cccgatccga aggtgccggt cagcggggcc tggtcgtcgg tgaagccgac gatctgcgcg
     1261 gactgcgaag gcactgcggt ggccacgctc agcgagaagt ccgccgaggc cgtgcccttg
     1321 ttgcctgcgg catcggcgat cacggccttg taggtgtggg cggtgccgtt ggccaggacc
     1381 ggcagcgcga aggtccatgc ggcaccgttc acggtggccg tgcccagcag aacggcgccc
     1441 tcgaatatct gcacgacgtc gccggccttc agacccgaga ccgtgccctt gagcaccggc
     1501 gtcgtgtcgt tcgtgctcgt gccgctcggg tagtcgccga cattgggcgc aacgtcgtcg
     1561 gtataggcgg tgatcgcgac ggcgttgtcg gcggtggggc cgctgttgtc gatgatcacg
     1621 acctgcgagc tcgacggggt cgcattgccg gcggcatcca cgacgcgcgc caggaaggta
     1681 tagctgccat cgggcagcga gacggccgtg ttgtcgaact ggtagttgtt gttgcccaca
     1741 ggcgtggcgt tgctccaggt cctgccgttg tccatcgaga tctgcaccgt ggcgccctgc
     1801 tccagcgcgg agtcgagcgt gatcgtcgcg accagcgtgc ggtcgctggt gacgaagtcg
     1861 tcggcgctcg tgccggtgtc ggtgctgatc gcgccgacgg cggccttggc cgtcggaggc
     1921 gtgacgtcca cgttgaccgt gaaggaaccc gagtgcgcgc tctcgttgcc cacggaatcg
     1981 gtggtcgtgg cggtgaagcg gtgcgcgccc tctgccagcg gcgtggtcgg cgtgaagctc
     2041 cagctgccgt cggccttcac cgtggcggag ccgaggaagc tgccgttgtc atagatcttg
     2101 atgaggtcgc cggcggtggc accgctgccg ccgagcgtgg gcgtggtgtc gtcgctgtat
     2161 gcgccgctgg cgagcgcgcc ctgcacgagg cccacgtcgt cgccgacgcc ggcgatcttc
     2221 ggcacttccg gggcggtcgt gtcgatggtc aggtcgatgg tggcttcgcc cttcttgccc
     2281 gcggcgttca ccgcggtggc ggtgtagctg tgcgtgccct cgccttgagc ggaaagcgtg
     2341 agacgccagc tgccgccggc atcggcggtc gtcgaaccga ggaccgtcgc gccttccttg
     2401 acggtgacga gcgcgccggc cgtcgccttg cccacgagcg tgggcgtcgt gtcgtcggtg
     2461 cgggcaccgg gagcgaggtt gcccttgatg ctgccctggt cgtcgacgac ctgctcgatg
     2521 ctgacgacga cgtccgcgag gtcgacggtg aactccagcg gcgcgctggc ggcgctggtg
     2581 ttgcccgcgg cgtcggtcac ggtcagggtg atgcgatgcg ctccgctggc cagcggcgtg
     2641 ctgggcgtga tgctccaggc gccgtcggtc gcgaccgtga tgctgccgat gagggcgccg
     2701 ccgtcgtaca ggcgaatggt gtcgccggcc ttcacgccgc tgccgctgaa ggtgggcgtg
     2761 gtgtcgtctg tcacgccgcc ggcggtgatc gcgccctgtg caggcccctg gtcgtccatg
     2821 gccgtgacca ctgcaggggt ggccggtgca gtggtgtcga gcacgatcgc ataggcgccg
     2881 gtgcgcgggc tgacttgccc tgcgccgtcg accgcctgcg cggtgaggtt cttggtgccg
     2941 tcgccgttca gggcgctggt ggtgacgctc caggtgccgt cggaggccac gctggtcgag
     3001 ccaacggccg cgccgtcggc atagacggtg accaccgtgc cgaccgtgcc cgtgccgcgc
     3061 agggtgggcg tggtgtcgtc ggtgctcgcg tcgggcgcca cgttgccggt gacgctgccc
     3121 ttgtcgtcgc tcacgccgat gatcgcgggt gccgccggcg gatcgcccac gagcgtgaag
     3181 gtcgacgcgg gcgtggccgg gccgatgttg cctgccgcat cgaccgggcg tgcgacgagg
     3241 ctgtgcgcgc ccagggcgag gggcacggca ggcgtgaatc gccaggtgcc atcggcgttc
     3301 accgcggtgc tgccgatgag attgccgttg tcgtagacgt tgatggccgt gacctgggcg
     3361 ggatcggccg tgccggtgta ctccggcttg ctgtcgtcgg tcttcgcgcc ggcagccagg
     3421 gggccctgga cggggccgac gtgatccagc acgcgcagct gggaggcatc gagggccgac
     3481 ggcggcgtgg tgtccaccgc gagcctgaaa tcgacggcgc gcgggctttc gttgcctgcc
     3541 gcgtcggtga gcgtgtagct gatgctgtgc gcgccttcgg ccagcgggaa tgtaggcatc
     3601 agcgtgcccg tgttcgggtc gaaggtcgcg gcgaccttcg cgccgtcgac gtacagcgac
     3661 ggcgtcaacc cggccgcgac ggagccgatc ttgaaaccgg gcgtggtgtc gtcggtgaat
     3721 gccgccgtgc tggccgtgtt ctgcacggca ccgaagttgt cggcatagcc cgagatggca
     3781 tccggcctcg ccggcggcgt cgtgtcgacg ttgatcacga agggcgccga atgcccgctc
//
LOCUS       DATCRO010000234         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_745302, whole genome shotgun sequence.
ACCESSION   DATCRO010000234 DATCRO010000000
VERSION     DATCRO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745302"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..106
                     /locus_tag="VIN75_06335"
                     /pseudo
     CDS             1..106
                     /locus_tag="VIN75_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
     gene            complement(97..2346)
                     /locus_tag="VIN75_06340"
                     /pseudo
     CDS             complement(97..2346)
                     /locus_tag="VIN75_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
     gene            complement(2360..2842)
                     /locus_tag="VIN75_06345"
     CDS             complement(2360..2842)
                     /locus_tag="VIN75_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8973818.1"
                     /translation="MNVPLSLTVNGKAVEASVDPRTLLVQFLREQLRLTGTHVGCDTA
                     QCGACTVHLDGRSVKSCSMLAVQAQGRAVTTIEGLAPAGELHPMQAAFRECHGLQCGF
                     CTPGMVMASVQLLADNPKATEDDIRCGLEGNLCRCTGYHNIVRAVQACQSGQVGSTTV
                     "
     gene            complement(2961..>3167)
                     /locus_tag="VIN75_06350"
     CDS             complement(2961..>3167)
                     /locus_tag="VIN75_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973819.1"
                     /translation="AVLWREDPASWKGHVGFFVREDGDDIVLLAGNQLGAVCEQRHPK
                     TTVLSYRWPAAALDNGRQPTTGKA"
BASE COUNT          432 a         1093 c         1150 g          492 t
ORIGIN      
        1 caccggcctg tacgagaccg gcctgcaacc gggcgagctg gtcaccgccg tgagcttccc
       61 ggtgccgcag gccgcgggct acgagaaatt ccgcaacccg gcctcgccgc agcccttgac
      121 gcccagcggg ttgtgcgtgc acggcgtgac ggtgtggtcg acctggtagc tgggcaggtc
      181 gtcggcgcgc ggcatgcagt agtccatgta cgagccggtc agcagctggc ccgattcggg
      241 gtcgtagcgg cagccctcga gcatcgcctg gcccaggccc tgcgcgatgc cgccgtgcac
      301 ctggccctgc acgatcatcg ggttgacgat gttgccgaag tcgtccaccg cggtgaagcg
      361 gtcgatgcgc gtgcggcccg tctccggatc gacctccacc tcgcagacat agctgcccgc
      421 cgggtaggtg aagttggtcg ggtcgtagaa cgcgttctcg ttcaggccgg gctcgagctt
      481 gtcgagcggg tagttgtgcg gcacgtaggc cgtgagcgcc acctggccga acgggatctt
      541 gcggtcggtg ccggccacct tgaactcgcc gttctcgaac tcgatgtccg agtccgcggc
      601 ctccagcagg tgcgcggcga tcttcctgcc cttggcgatc accttgtcga ccgccttgac
      661 gatggccgtg ccgcccaccg ccagcgagcg gctgccgtag gtgcccatgc cgaacgggat
      721 gcgaccggtg tcgccgtgca cgatctcgac gctctcgacc gggatgccca gccgcgcggc
      781 gaccacctgc gcgaaggtcg tctcgtggcc ctggccatgg ctgtgcgagc cggtgaacac
      841 ggtgacggtg cccgtcgggt gcacgcgcac ctcgcccgcc tcgaacaggc cggcgcgcgc
      901 gccgagcgcc cccgcgatgt tgctgggcgc caggccgcag gcctcgatgt agcagctgta
      961 gcccaggccc cgcagcttgc ccgcggcccg ggactgctgg cgccgcgcgg cgaagccggc
     1021 gacgtccgcc agttgcagcg cgcggtccag gtgggcggca tagttgccgg tgtcgtaggt
     1081 caggccgacc ggggtcgcgt acgggaactc ggtgatgaag ttgcgtcggc ggatgtcgac
     1141 cgggctcatg ccgaggtccc gcgccgcggt ctccacgagc cgctcgacga cgtaggtcgc
     1201 ctcgggccgg ccggcgccgc gataggcgtc gacgggcgcc gtgttcgtga agaccgcggt
     1261 gacctgggca tggatcgcgg gcgtcctgta ctgccccgcc agcagcgtcg cgtacaggat
     1321 cgtcggcacg ctggacgcga aggtcgacag gtacgcgccc aggttggcgg tcgtcttcac
     1381 gcgcatcgcg aggaagtcgc cctgctcgtt cagcgcgagt tcggcggtcg tcaggtggtc
     1441 gcggccgtgg gcgtcggtca ggaacgactc gctgcgctcg gccgtccact tgatgggccg
     1501 gcccacgcgc ttggacgccc agaccagcgc cgtctcctcg ggatagagga agatcttcga
     1561 gccgaagccg ccgcccacgt cgggcgcgat cacgcgcacc ttggactcgg gcaggcccag
     1621 cacgaaggcc gtcatcagca gccgctcgac gtgcgggttc tggttggcga cccacagcgt
     1681 gtagctgtcg tcatgacgcg tgtactgcgc gttggcggcg cgcggctcga tcgcgttggg
     1741 caccaggcgg ttgttggcga actccagctt cgtgacccgc gcggcgcgcg cgaacgcggc
     1801 gtccacggcc gccttgtcgc catgggacca gtcgtagcag acgttgccgg gcacctcgtc
     1861 gtgcaccgcc gacgcagcgc cggcggcggt cttgaggtcg acgaccgcgg gcaattcctc
     1921 gtagtcgacc gccaccagct cggccgcgtc cttggcctgg agcagcgtct cggcgacgac
     1981 gagcgccagc tggtcgccga cgtggcgcac cttgcgctgc gccagcaccg ggtgcggcgg
     2041 ctccttcatg ggcgagccgt cctggctgtg gatgagccag ccgcagggca ggccgccgac
     2101 cttggcgtcg gccaggtcgg cgccggtgaa gaccgcgagc acgccgggcg cggcccgggc
     2161 cgccgacgtg tcgatggact tgatgcgggc atgcgcgtac ggcgagcgca cgaacaccgc
     2221 gtaggtctgg cccggcagga cgacgtcgtc cgtgtactgg ccggcgccgg tgaggaagcg
     2281 cgcgtcctcg cggcgcttga gcggctggcc gatgagcgag gcgggcggct gcgagaggtc
     2341 ggtcatggca acgcctcctt cagacggtgg tggagccgac ctggccggac tggcaggcct
     2401 gcaccgcgcg cacgatgttg tggtatcccg tgcagcggca caggttgcct tcgaggccgc
     2461 aacggatgtc gtcctcggtc gccttggggt tgtcggcgag caactgcacc gaggccatca
     2521 ccatgccggg cgtgcagaag ccgcactgca ggccatggca ttcgcggaag gcggcctgca
     2581 tcggatgcag ctcgcccgcg ggcgccaggc cctcgatggt cgtcaccgcg cggccctggg
     2641 cctgcacggc gagcatcgaa caggacttca cggatcgccc gtccaggtgg accgtgcagg
     2701 cgccgcactg ggcggtgtcg cagccgacat gggtgccggt cagccgcagt tgctcgcgca
     2761 ggaactggac cagcaacgtg cggggatcga cgctcgcctc gaccgctttt ccgttgacgg
     2821 tcaacgacag tggaacgttc acgggaagtc tcctcatgtg gcgggcgcgg ggccctcggg
     2881 acctgtgaaa gaaccggcgt gcctcgtcac gaggcgcgtc tgcgctgccg atcttccgcg
     2941 ccccggcggc ccgccaacac ctaggcttta cccgtagtcg gctggcggcc gttgtcgagc
     3001 gccgccgccg gccagcgata cgacaacacg gtcgtcttcg gatggcgctg ctcgcacacc
     3061 gcgccgagct ggttgccggc cagcagcacg atgtcgtcgc cgtcctcgcg cacgaagaag
     3121 ccgacgtggc ccttccagct ggccggatcc tcgcgccaca gcacggc
//
LOCUS       DATCRO010000235         4893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_452456, whole genome shotgun sequence.
ACCESSION   DATCRO010000235 DATCRO010000000
VERSION     DATCRO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452456"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VIN75_06355"
     CDS             <1..357
                     /locus_tag="VIN75_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973820.1"
                     /translation="ATNYAVEGATGEWSCVVCSQDTYARSLPDEIRSPAMRWLKVHAE
                     YDDTHPWEALDIIATLLGHAPSAHEIAQVRQAIRTSYRYMELSLDSAMMASIHGTFDE
                     TASNSSMLGVEALNVA"
     gene            complement(371..1141)
                     /locus_tag="VIN75_06360"
     CDS             complement(371..1141)
                     /locus_tag="VIN75_06360"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEY8973821.1"
                     /translation="MLSGFTPPYTPSGRSALIPAPPWHYAGQLLSLAFDVDRAAAQSL
                     LPDGLGTATGGAAAHFCEWQSTTDGSELLDPAYSQYREFLALVEVERQGTRCFYCPFI
                     YVDQDLSMARGWLQGWPKKAGSVWLTRSYDLEHPAAAPARAGSRFGASLAVKDRRLAE
                     AAFTLTGEAAAPIGFLAGPSYGLIPTASVLGAPDRGTPTLARSAVQGRVQGPAHAATG
                     TLRFLESPRDELALLQPERVTAASLSTLAMTITGAIAG"
     gene            1272..1757
                     /locus_tag="VIN75_06365"
     CDS             1272..1757
                     /locus_tag="VIN75_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8973822.1"
                     /translation="MPRDIDAHDSRILAALQRNGRLTVAELSEKVHLSTSPTWRRVKR
                     LEDEGVIVGYEAKLDRRALGWGVLAFVMVSIGDHGEAEAIAFERAVAGLPEIVACWSI
                     AGSSDFLLQVVKQDLDTYADFAMTTVRRLPGIKAMQTILTLKEVKSPAPWPVPAADAA
                     A"
     gene            1911..3416
                     /locus_tag="VIN75_06370"
     CDS             1911..3416
                     /locus_tag="VIN75_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY8973823.1"
                     /translation="MTPHSREPLRAATGVNGLDDVLHGGLIADRLYLIDGNPGAGKTT
                     LALQFLLEGRRRGEPTMYVTLSETAEELRDGAASHGWSLDGIHIVELHGEEGSDGGID
                     NELTMFHPSEVELSQTTRLVLAAVERHAPKRMVFDSLSELRLLAQNSLRYRRQILALK
                     QFFAGRACTTLFLDDRTADGSDLQLQSIAHGVISLEHQAPVYGRALRHLRVVKFRGSD
                     FRTGFHHLRLLRGGLEVYPRLTASDHGEAFDVVHLASGVQTLDRLLGGGIDIGTSTLI
                     VGPAGSGKSTIALQYAIAATRRGEHAVVFTFDESRQSIAGRLRGMGMSVEGEGAPLRI
                     CQVDPAEISPGEFAHMVRDAVEKDRARIVVIDSLNGYINAMVDGHYLTAQLHELLSYA
                     GNHGVSTFVIAAQTGMIAGQMGAPGDASYLADTVIYTRFFELAGQLRKAISVLKKRSG
                     PHEHTIRELLFDGKGVQLSEPLRQFHGILSGTPVETSSPEQSSTAASRATP"
     gene            3416..4615
                     /locus_tag="VIN75_06375"
     CDS             3416..4615
                     /locus_tag="VIN75_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8973824.1"
                     /translation="MDMLTTPSGAELRVLMAPATRRDGETTAQALAPLGVQVEVAADP
                     DSIAEQVDAGVGAILLTELALAGTGLDRLVRTLDAQPAWSDVPIVLALRSDAPAPVTA
                     LCGRLSNVTLIDRPTSIRTLANAVQAALRARRRQYEIRDRIAALERAEQALRDADRRK
                     DQFLATLAHELRNPLAPIRNAAYLLGNVALAPERVAECASMIARQSRSMAMLLDDLLD
                     VARITNGKLELKFIDVTLQSVVDSAVEAVRPLVDARRHRFAASVADPRAVVSADPLRL
                     SQVLTNVLANAAKYTDPGGNIELAVRVVGDVAEFVVRDDGVGIDRALLPYLFEMFNQA
                     TDTLDRSEGGLGIGLSLARSLIEMHGGEITASSARCLSSGWPQPVVATSSTCRPHAWA
                     RTRRATS"
     gene            4585..>4893
                     /locus_tag="VIN75_06380"
     CDS             4585..>4893
                     /locus_tag="VIN75_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973825.1"
                     /translation="MGAHAARDLVAVGAGHADVHHHRGRRRRLGARDRLQAVVRDLDV
                     VAIELQHHRDGGRGIDVVVGDEDAHLRAGVRRDDRVGPPPRGNVAATRRDHRQRQPER
                     R"
BASE COUNT          722 a         1763 c         1705 g          703 t
ORIGIN      
        1 gccaccaact acgccgtcga aggcgccacg ggcgagtggt cctgcgtggt gtgctcgcag
       61 gacacctacg cccggtcgct gcccgacgag atccgctcgc cggcgatgcg ctggctcaag
      121 gtgcacgccg agtacgacga cacgcacccg tgggaggcgc tggacatcat cgccacgctg
      181 ctgggccacg ccccgagcgc gcacgagatc gcgcaggtgc gccaggcgat ccgcaccagc
      241 taccgctaca tggagctgtc gctcgacagc gccatgatgg cctcgatcca cgggacgttc
      301 gacgagacgg cgtccaattc gtcgatgctg ggcgtcgagg cgctgaacgt ggcgtgacgg
      361 cgcgtggcgg tcacccggcg atggcgccgg tgatcgtcat cgccagcgtg gacaggctcg
      421 cggccgtcac ccgctccggc tgcagcagcg cgagctcgtc gcgcgggctc tcgaggaagc
      481 gcaacgtgcc ggtggccgcg tgcgccgggc cctgcacgcg cccttgcacc gcggagcgcg
      541 ccagcgtcgg cgtgccgcga tccggcgcac ccagcaccga ggccgtcggg atgaggccgt
      601 agctcggccc cgccaggaag ccgatgggcg cagcggcctc gccggtcaac gtgaacgcgg
      661 cctcggcgag ccggcgatcc ttgaccgcga ggctcgcgcc gaagcggctg cctgcgcgcg
      721 cgggcgcggc ggccgggtgc tcgaggtcgt agctgcgcgt cagccacacg ctgccggcct
      781 tcttgggcca gccctgcagc cagccgcgcg ccatcgacag gtcctggtcg acatagatga
      841 acgggcagta gaagcagcgc gtgccctggc gctcgacctc gacgagcgcg aggaactcgc
      901 ggtactgcga ataggccgga tcgagcagtt cgctgccatc ggtggtcgat tgccactcgc
      961 agaagtgcgc ggcggctccg cccgtggcgg tgcccaggcc gtccggcagc agcgactgcg
     1021 ccgcggcgcg gtccacgtcg aatgccagcg acagcagttg tcccgcgtag tgccagggcg
     1081 gggcggggat gagcgccgag cggcccgacg gcgtgtacgg cggcgtgaag cctgaaagca
     1141 tgcgggtccc ttcgtgtgat tggtggaggc catgctattg cgaggtgtcg ggaaaaggtt
     1201 gcctttccat gcctgtgcag gtgcgcggtt gggcatgaag cctatgatcc aggcaacaaa
     1261 ctggaatgcc catgcctcgc gacatcgacg cccacgattc ccgcatcctc gccgcgctcc
     1321 agcgcaacgg ccgcctgacg gtggccgagc tctccgagaa ggtccacctg tccacctcgc
     1381 cgacctggcg ccgcgtcaag cggctggaag acgagggcgt catcgtcggc tacgaggcca
     1441 agctcgatcg ccgggccctc ggctggggcg tgctcgcgtt cgtgatggtc tccatcggcg
     1501 accacggcga ggccgaggcg atcgcgttcg agcgcgcggt ggccggcctg ccggagatcg
     1561 tcgcttgctg gagcatcgcg ggcagctcgg acttcctgct gcaggtggtc aagcaggacc
     1621 tggataccta cgccgacttc gccatgacga ccgtgcgccg cctgcccggc atcaaggcga
     1681 tgcagacgat cctgacgctc aaggaagtga agtcgccggc gccctggccg gtgccggcag
     1741 cggacgcggc ggcctgacga gacgaggggc actgcaggcg ctatccttgt caccttttcg
     1801 atagttgcta ctgtcgcgca cgccgattgc caagggtgcc ggcaaaccgt cgcccgtcgt
     1861 tccggcctgg cgcagcgatg gccgttccac gatgacgccg gagagacgac atgaccccac
     1921 actcccgcga acctctgcgc gccgcgaccg gcgtcaacgg cctggacgac gtgctccatg
     1981 gcggcctgat cgccgaccgc ctctacctga tcgacggcaa ccccggcgcc ggcaagacca
     2041 cgctcgccct gcagttcctg ctggagggcc gtcgccgcgg cgagcccacc atgtacgtga
     2101 ccctgtccga gacggcggag gaattgcgcg acggcgcggc gtcgcacggc tggtcgctgg
     2161 acggcatcca catcgtcgag ctccatggcg aggagggcag cgacgggggc atcgacaacg
     2221 aactgacgat gttccatccg tccgaggtgg agctgagcca gaccacgcgc ctggtgctcg
     2281 cggcggtcga acggcacgcg cccaagcgca tggtgttcga ctcgctgtcg gagttgcggc
     2341 tgctggcgca gaactcgctg cgctaccggc gccagatcct ggcgctcaag cagttcttcg
     2401 ccggccgcgc ctgcacgacg ctgttcctgg acgaccgcac ggccgacggc tccgacctgc
     2461 agctccagag catcgcgcac ggcgtcatct cgctggagca ccaggcgccg gtctacggtc
     2521 gcgccctgcg gcacctgcgc gtcgtcaagt tccggggcag cgactttcgc accggcttcc
     2581 atcacctgcg gctgctgcgc ggcgggctgg aggtctatcc gcgcctgacc gcttccgacc
     2641 atggcgaggc cttcgacgtc gtccacctgg ccagcggcgt gcaaacgctg gaccgcctgc
     2701 tgggcggcgg catcgacatc ggcaccagca cgctgatcgt cggccccgcg ggctcgggca
     2761 agtccaccat cgcgctgcag tacgcgatcg ccgcgacgcg tcgcggcgaa cacgccgtgg
     2821 tgttcacgtt cgacgagtcg cgccagagca ttgccggccg ccttcggggc atgggcatgt
     2881 ccgtcgaagg cgagggcgcc cccctcagaa tctgccaggt ggaccccgcc gagatatcgc
     2941 ccggcgagtt cgcgcacatg gtccgcgacg cggtggagaa ggaccgcgcg cggatcgtgg
     3001 tgatcgacag cctcaacggc tacatcaacg cgatggtcga cggccactac ctgaccgcgc
     3061 aactgcatga gctgctctcg tacgccggca accacggcgt gtcgaccttc gtgatcgccg
     3121 cccagaccgg catgatcgcc ggccagatgg gggcgccggg cgacgccagc tacctcgccg
     3181 acactgtcat ctacacgcgc ttcttcgagc tcgccggcca gctgcgcaag gcgatctcgg
     3241 tgctcaagaa gcgcagcggc ccgcacgagc acacgatccg cgaactgctt ttcgatggca
     3301 agggcgtgca gctgagcgag cccctgcggc agttccacgg catcctctcg ggcacgcccg
     3361 tggagacctc gagccccgaa cagtcttcca ccgccgcctc gcgagcgacc ccctgatgga
     3421 catgctcacg acgcccagcg gcgccgagct tcgcgtcctc atggcgccgg ccacccggcg
     3481 cgacggcgag accaccgcgc aggcgctggc gccgctgggc gtgcaggtgg aggtggccgc
     3541 cgacccggac agcatcgccg agcaggtcga cgccggcgtc ggcgccatcc tcctgaccga
     3601 gctggcgctg gccggcacgg gtctcgatcg gctcgtgcgc acgctcgatg cacagccggc
     3661 ctggtcggac gtgcccatcg tgctggccct gcgcagcgac gcgcccgccc ccgtcacggc
     3721 cctctgtggc cgcctgagca acgtcacctt gatcgatcgc ccgacctcga tccgcacgct
     3781 ggccaatgcg gtgcaggcgg ccctgcgcgc gcgtcgccgg cagtacgaga tccgcgatcg
     3841 catcgccgcg ctcgagcgcg ccgagcaggc gctgcgcgat gcggaccggc gcaaggacca
     3901 gttcctggcc acgctcgcgc acgagctgcg caatccgctg gccccgatcc gcaacgcggc
     3961 ctacctgctg ggcaacgtgg cgctcgcgcc ggagcgcgtg gccgagtgcg cctcgatgat
     4021 cgcgcgccag tcgcgctcga tggcgatgct gctggacgac ctgctggacg tcgcgcgcat
     4081 caccaacggc aagctggagc tcaagttcat cgacgtgacg ctgcagtcag tcgtcgacag
     4141 cgccgtcgag gcggtgcgcc cgctggtcga cgcgcgccgg catcgctttg cggcgtcggt
     4201 ggccgatccg cgcgcggtgg tgagcgcgga tccgctgcgc ctgtcgcagg tgctgaccaa
     4261 cgtgctggcc aacgccgcca agtacaccga ccccggcggg aacatcgagc tcgcggtgcg
     4321 ggtggtcggc gacgtggccg agttcgtcgt ccgcgacgac ggcgtgggca tcgaccgcgc
     4381 gctgctgccc tacctgttcg agatgttcaa ccaggccacc gacacgctcg atcgttcgga
     4441 gggcggcctg ggcatcgggc tgtcgctggc gcgctcgctg atcgagatgc acggcggcga
     4501 gatcaccgcc tcgagcgcgc gctgcttgtc ttccggctgg ccccaaccgg tggtggcgac
     4561 cagcagcacg tgccggcccc acgcgtgggc gcgcacgcgg cgcgcgacct cgtagccgtc
     4621 ggcgccgggc atgccgatgt ccaccaccat cgcggccggc gtcgccgcct cggcgcgcgc
     4681 gatcgcctgc aggccgtcgt gcgcgatctc gacgtcgtag ccatcgagct ccagcatcat
     4741 cgcgacggag gccgcggcat cgacgttgtc gtcggcgacg aggacgcgca cctgcgcgcc
     4801 ggtgtccgtc gcgatgaccg cgtcggcccc ccgccccgcg ggaacgtggc tgcgacgagg
     4861 cgcgaccacc ggcagcgtca gcctgaacgt cgc
//
LOCUS       DATCRO010000236         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_47627, whole genome shotgun sequence.
ACCESSION   DATCRO010000236 DATCRO010000000
VERSION     DATCRO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47627"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIN75_06385"
     CDS             complement(<1..471)
                     /locus_tag="VIN75_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973826.1"
                     /translation="MLDAAGRERVASDDNNHRDRAFGRDAHAAGTSHRVRRRPSHGQL
                     FREAARREAAARHRDEQRLVWALLLSLAFHFLLFLLQFDAPGIGLPGITHPLKERRVL
                     VPELTIVLAPRREDAASAGGARAADAGAAPVAMTATQTRDAGWSATVPAPGPAPA"
     gene            complement(580..1158)
                     /locus_tag="VIN75_06390"
     CDS             complement(580..1158)
                     /locus_tag="VIN75_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8973827.1"
                     /translation="MIDGMDPLPTPGQPHAPSRRAATGWILACAGWPLVARAGADNAV
                     QGAVRIGQPLRDGPLNGLNGPAHRLSDFRGHPLIINVWASWCGPCRREMASLERLAWR
                     GDARAFDIIGISTDDDPAAAKRLLASTNATIAQFIDQRLYWETMLGASQIPLTVLVDS
                     RGTLLRRVYGAQEWDGAEAQRLIAGTFVETLR"
     gene            1145..2326
                     /locus_tag="VIN75_06395"
     CDS             1145..2326
                     /locus_tag="VIN75_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973828.1"
                     /translation="MPSIMRECPRAAGGAPWRFCLSRGCAQARKCFAGRVPIGQNLPE
                     RFRQCQSAGGAGHTEGIMNRESERPMHAGMPAAGRVYRSGDDLLRDLDGGAGPHARAM
                     ATGYWMAIHDADASCLTATELLKYLQSETGSVAELTGLGYVQAIFDCSEPGEKRRMPK
                     EGPDGEAAARREAARVKAWLEHRPATVMQPALTAVQLALAGSPLPDPVRRPAARAPAG
                     VFGAIRAQSWPAKGMAVAALALVFVVGVQQVSTARAAERAAAFDLAVGRQAEYMTAML
                     LEQRRYEKDSILNMSDPAEIEAYANQWEKARVAMQTSLDTLGAANLADEDRQSLTAIR
                     DDSRTYEQGYLQVLAQMRQGHVRSPQEANRMLEAYKPAAHRAEVNGVLLATRAMQRLH
                     Q"
     gene            2476..2934
                     /locus_tag="VIN75_06400"
     CDS             2476..2934
                     /locus_tag="VIN75_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY8973829.1"
                     /translation="MPSIIRHAAAVFLLAACACAFADVTVEAPWIRGVVAGQSSTGAF
                     MTLRSTEATELVAMSSPAAESLQVHKTAMVDGMMTMEPVDALAVPAHGSVALEPGGLH
                     VMLVGLRVPLRPGDKVPLTLTFRGADRRESAVTVQAEVRDVTGAPSHGKR"
     gene            2945..3550
                     /locus_tag="VIN75_06405"
     CDS             2945..3550
                     /locus_tag="VIN75_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8973830.1"
                     /translation="MTATSMPARLRRRSLLVLAGGLVAGRTAAATPSSGKDVSGEHLG
                     GVLDLPDAAGRVRRLAEFRGKVLLLFFGYTRCPDVCPTTLVRVAEAVRRLAPDEASRV
                     RVLWATVDPERDSPELVGRYVQAFNPAFIGLSGSVAQTDVVAAAFRFRYDVTTYRNDV
                     LVSHSPDGFVIDARGRTRVRIGVDATPEQIERDVHALLAET"
BASE COUNT          512 a         1267 c         1350 g          513 t
ORIGIN      
        1 ggcgggcgcc gggcccggcg ccgggacggt cgccgaccac cccgcgtccc gcgtctgcgt
       61 cgcggtcatc gccacggggg ccgcgccggc gtcggccgcg cgcgccccgc ccgcggaagc
      121 ggcgtcttcc cgtcgcggcg ccaggacgat cgtcagctcc ggcaccagga cccggcgttc
      181 cttcaacggg tgcgtgatgc ccggcaatcc gatccccggc gcgtcgaact gcagcaggaa
      241 cagcaggaaa tggaacgcca gcgacagcag cagggcccag accaggcgct gctcgtcccg
      301 atggcgcgcc gcggcctcgc ggcgagcggc ttcacggaac agctggccgt ggctgggccg
      361 ccggcggacg cggtgcgacg tcccggcggc gtgcgcatcg cgcccgaagg cacgatcgcg
      421 gtggttgttg tcgtccgagg ccacgcgttc cctgcccgcc gcatccagca tgcatcactc
      481 gcggggcgtc cgatgctacg cccacgcgac gccacgcgcg tgtcaagacg tcatcgaccg
      541 cgggcggcgg ctgcagcgcc ggggcgggtc ggcccgcctt cagcgtaggg tctcgacgaa
      601 ggtgccggcg atcagccgct gcgcctcggc gccgtcccat tcctgcgccc cgtacacgcg
      661 ccgcagcagg gtgcctcggg aatcgaccag cacggtcaag ggaatctgcg aggcgccgag
      721 catggtctcc cagtacaggc gctggtcgat gaactgcgcg atcgtggcgt tcgtcgacgc
      781 cagcaggcgc ttcgccgcgg ccgggtcgtc gtcggtggag atgccgatga tgtcgaacgc
      841 ccgggcatcg ccacgccagg ccaggcgctc gagcgacgcc atctcccgcc ggcagggccc
      901 gcaccagctg gcccagacat tgatgatcaa cggatggccg cgaaagtcgg agagccggtg
      961 ggccggaccg ttgaggccgt tgagcgggcc gtcgcgcagc ggctggccga tccgcacggc
     1021 cccttgcacg gcattgtcgg cgccggcccg cgcgacgaga ggccatcccg cgcatgcgag
     1081 gatccatccc gtggcggcgc ggcgcgaagg cgcgtgcggt tgtccagggg tggggagggg
     1141 gtccatgccg tcgatcatgc gggaatgccc cagggccgca gggggggcgc cctggcgatt
     1201 ctgcctgtcc cgcggctgtg cgcaggcacg caaatgcttt gcaggacgag tgccgatcgg
     1261 tcagaatttg cctgaacggt tccgccagtg tcaatcggct ggtggagccg gccacacgga
     1321 gggcatcatg aatcgcgaat ccgagcgccc gatgcatgcc ggcatgccgg ccgccggccg
     1381 cgtgtaccgg tcgggcgatg atctgctgcg ggacctcgat ggcggtgcgg gcccccacgc
     1441 gcgggcgatg gccaccggct actggatggc gatccacgat gccgacgcca gttgcctcac
     1501 tgccaccgag ttgctgaagt acctgcagtc ggagaccggc agcgtggccg agctgaccgg
     1561 tctgggctac gtgcaggcca tattcgactg ctcggagccc ggggagaagc gtcgcatgcc
     1621 caaggaaggg ccggacggcg aggccgccgc gcgccgcgag gcggctcggg tcaaggcttg
     1681 gctggaacat cggccggcca cggtcatgca gcctgcattg acggccgtgc agctggccct
     1741 ggccggttcg ccgctcccgg atccggtgcg acggccggcg gccagggcgc cggccggcgt
     1801 gttcggcgcg atcagggcgc agtcctggcc ggccaagggc atggcggtcg ccgcgctggc
     1861 gctcgtcttc gtggtcggcg tgcagcaggt gtccaccgcc cgcgcggccg agcgggccgc
     1921 cgccttcgac ctcgccgtcg gacggcaggc ggagtacatg acggccatgc tgctggagca
     1981 gcgccgttac gagaaggact ccatcctcaa catgtccgat cccgcggaga tcgaggccta
     2041 cgcgaaccaa tgggagaagg cgcgggtcgc gatgcagacc agcctggaca cgctcggcgc
     2101 ggccaacctg gccgacgagg atcggcagtc gctgaccgcg atccgggacg actcccgcac
     2161 ctacgagcag ggttacctgc aggtgctcgc gcagatgcgc cagggtcacg tgcgttcgcc
     2221 gcaggaggcg aaccgcatgc tcgaggccta caagcccgcg gcacatcgcg ccgaggtcaa
     2281 cggcgtgctc ctcgcgacgc gcgcaatgca gcgcctgcat cagtgaccga gggacggggt
     2341 gccgcgcagg acgtgtccgg catgtctcgg aatcgtctga ccgtatcaac gccatcgaca
     2401 agcggcgcgg ccatcgctat aagttcctga cgacattgga tcgcggcgcg atgcgcgatt
     2461 cgaaaggagg gagcgatgcc atcgatcatc cgccatgcgg ccgcggtgtt cctgctggcg
     2521 gcctgtgcat gcgcgttcgc tgacgtgacg gtcgaggcgc cctggatacg cggcgtcgtc
     2581 gccggccagt ccagcaccgg cgccttcatg accttgcgca gcaccgaggc gacggagctc
     2641 gtcgcgatgt cgtctcccgc ggccgaaagc ctgcaggtgc acaagaccgc catggtcgac
     2701 ggcatgatga ccatggagcc cgtcgacgcg ctggcggtgc cggcccatgg cagtgtcgcg
     2761 ctcgagccgg gcggcctgca cgtgatgctg gtgggcttgc gggtgccgct gcgtcccggc
     2821 gacaaggtgc ccttgacgct gacctttcgc ggcgcggacc ggcgcgagag cgcggtgacc
     2881 gtccaggccg aggtgcgcga cgtcaccggc gcgccctcgc acggcaagcg ctgaaggagc
     2941 ggacgtgacg gcgacgagca tgcccgcccg cctgcgcagg cggtcgctgt tggtgttggc
     3001 cggcggcctg gtcgcgggcc gcaccgcggc ggcgacgcct tcgagcggca aggatgtctc
     3061 cggcgagcac ctgggcggcg tcctcgacct gcccgacgcc gccgggcggg tgcgccggct
     3121 cgcggaattc cggggcaagg tgctgctgct gttcttcggc tacacacgct gccccgacgt
     3181 gtgtcccacc accctggtgc gcgtggccga ggccgtgcgg cggcttgcgc cggacgaggc
     3241 ctcgcgcgtg cgggtgctct gggcgacggt cgacccggaa cgcgactcgc ccgaactcgt
     3301 gggccgctac gtgcaggcat tcaatcccgc cttcatcggc ctgagcggct cggtggccca
     3361 gaccgacgtg gtggccgcgg ccttccgctt ccgctatgac gtcaccacgt accgcaacga
     3421 cgtgctcgtg agccattcgc ccgatggctt cgtgatcgac gcgcgcggcc gcacgcgtgt
     3481 gcgcatcggc gtcgacgcga cgcctgaaca gatcgaacgc gacgtgcacg cgctcctcgc
     3541 ggagacctga gcgcttgcga ccccggctgg cgcgtcgtgg ccgtcaggct ggaccaggcg
     3601 ccgtgtggcg gtcgggaggg tgcgtggggg ggatccctcg cg
//
LOCUS       DATCRO010000237         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_868690, whole genome shotgun sequence.
ACCESSION   DATCRO010000237 DATCRO010000000
VERSION     DATCRO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868690"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2381)
                     /locus_tag="VIN75_06410"
     CDS             complement(<1..2381)
                     /locus_tag="VIN75_06410"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY8973831.1"
                     /translation="MAASNLKLKNLHELVPQDVDVDAPRPAGNASALLARYREAGYLK
                     AGVNPLRLARQGDIEPAPPAFRHAAHGEADLPGLEARLRRTYCEGLALNAAHVRNHAR
                     VDWLYERLEARAAAPRVAPDQRLALYELLFAAERLERAIAADYPAAKRFSLEGSESYL
                     VFLRAAIEAAATHGAGQVVMGMPHRGRVNVLANVMGLSADELRSLFTETPAPHLAAWD
                     IKEHLGLSRRLRTDAGWIDVLLAHNPSHLESVTPVVTGMVRALQERAAANPRGHVVPL
                     IVHGDASFSGQGIVTETLNLAGTRGYGVGGTVHVILNNQIGSTVSNLADARSTLNSAD
                     VARAYDVPVLHVNGDMPELVAQAAELALQYRQRFHADILVDIVGYRRHGHNGHDDPRV
                     TQPAMQRVVRSMPTVVQRYRAQLAREGEPALERIVACEERVAGAQSLPLCAVDIAPVD
                     ATPAAAAAPRVAMTAVAGVPFARLKALADTMSAPPANFRLHDDIVALAAGWRETAARR
                     ANGVDWCLAETLAYASVLSAGANVRLSGLDIGRGSFFHRQCVWHDQDALVDGEKVHVP
                     LRHLGERQGVFSVFETPLSEEAVLGFEYGYSVTSADTLVAWEAQFGDFVNNAQVLIDQ
                     FIASGEAKWGWRSRLAMLLPHGNEGGGPEHSSAYVGRFLSLCAEHNMIVCMPSTSAQM
                     FHLLRRQASSASPKPLVVFTPKGQLYGTKASFSAWSEFEGGGFQALLGDIDPAQAPAV
                     ERVVLCSGKIHYALAADLAARPDPRVALLRVEQLYPLPAAEIGQRLALLPRLA"
BASE COUNT          363 a          977 c         1000 g          389 t
ORIGIN      
        1 tcggccagcc tgggcaggag cgcgagccgc tggccgatct cggcggcggg cagcggatag
       61 agctgctcga cgcgcagcag cgcgacgcgc gggtccggcc tggcggcaag gtcggccgcc
      121 agcgcgtagt ggatcttgcc gctgcacagc accacgcgct cgaccgccgg cgcctgcgcg
      181 ggatcgatgt cgccgagcag ggcctggaag ccgccgccct cgaactcgct ccacgccgag
      241 aagctcgcct tcgtgccgta cagctggccc ttgggcgtga acaccaccag cggcttgggg
      301 ctggccgagc tcgcctgccg gcgcagcagg tggaacatct gcgccgacgt gctgggcatg
      361 cacacgatca tgttgtgctc ggcgcacagc gacaggaagc ggccgacgta ggcgctcgaa
      421 tgctccgggc cgccgccctc gttgccgtgc ggcagcagca tcgccagccg gctgcgccag
      481 ccccacttgg cctcgcccga ggcgatgaac tggtcgatca gcacctgcgc gttgttgacg
      541 aagtcgccga actgcgcctc ccaggcgacg agggtgtcgg cgctcgtcac cgaatagccg
      601 tactcgaagc ccagcaccgc ctcctcggac agcggcgtct cgaacaccga gaacacgccc
      661 tggcgctcgc ccaggtggcg cagcggcacg tgcaccttct cgccgtcgac cagcgcgtcc
      721 tggtcgtgcc acacgcactg gcggtggaag aagctgccgc ggccgatgtc caggccgctc
      781 aggcgcacgt tggcgccggc gctcagcacc gacgcgtagg ccagcgtctc ggccaggcac
      841 cagtcgacgc cgttggcgcg ccgcgcggcg gtctcgcgcc agcccgccgc cagcgccacg
      901 atgtcgtcgt gcagccggaa gttggccggc ggcgcgctca tcgtgtcggc gagcgccttg
      961 agccgggcga agggcacgcc ggccacggcg gtcatcgcga cgcgcggcgc cgcggcagcg
     1021 gccggcgtgg catcgacggg ggcgatgtcg acggcgcaca gcggcagcga ctgcgcgccg
     1081 gccacgcgct cctcgcacgc gacgatgcgc tccagcgccg gctcgccctc gcgcgccagc
     1141 tgcgctcgat agcgttgcac caccgtcggc atcgagcgca ccacgcgctg catcgccggc
     1201 tgcgtcaccc gcgggtcgtc atggccgttg tggccgtggc ggcggtagcc cacgatgtcg
     1261 accaggatgt ccgcgtggaa gcgctggcgg tattgcaggg ccagttcggc ggcctgtgcc
     1321 acgagctcgg gcatgtcgcc gttgacgtgc agcacgggca cgtcgtacgc gcgggcgacg
     1381 tcggcgctgt tcagcgtgga gcgcgcgtcg gccaggttcg acacggtcga tccgatctgg
     1441 ttgttcagga tgacgtgcac ggtgccgccc acgccgtagc cgcgcgtgcc ggccaggttg
     1501 agcgtctcgg tcacgatgcc ctggccgctg aacgaggcgt cgccgtgcac gatcagcggc
     1561 acgacgtggc cgcgcggatt cgcggccgcg cgttcctgca gcgcgcgcac catgccggtg
     1621 acgacgggcg tgacgctctc caggtgcgac gggttgtgcg ccagcagcac gtcgatccag
     1681 cccgcgtccg tgcgcaggcg gcgcgacagc cccaggtgtt ccttgatgtc ccaggccgcc
     1741 aggtgcggcg cgggcgtctc cgtgaacagc gagcgcagct cgtcggccga caggcccatc
     1801 acgttggcca gcacgttcac gcggccgcgg tgcggcatgc ccatcacgac ctgcccggcg
     1861 ccgtgggtgg ccgcggcctc gatcgcggcg cgcaggaaca cgaggtagga ctcgctgccc
     1921 tccaggctga agcgcttggc cgcggggtag tcggccgcga tggcgcgctc gaggcgctcg
     1981 gccgcgaaca gcagctcgta cagcgcgagg cgctggtccg gcgcgacgcg cggcgccgcg
     2041 gcgcgtgcct cgagccgctc gtagagccag tcgacgcgcg catggttgcg cacgtgcgcg
     2101 gcgttcagcg cgaggccctc gcagtaggtg cgccgcagcc gcgcctccag gccgggcaga
     2161 tccgcttcgc cgtgcgcggc gtgccggaag gccggcggcg cgggctcgat gtcgccttgg
     2221 cgcgcgaggc gcagcggatt gacgccggcc ttgaggtatc ccgcctcgcg gtagcgcgcc
     2281 agcagcgcgg acgcgttgcc cgcgggccgc ggggcgtcga cgtcgacgtc ctgcggcacg
     2341 agctcgtgca ggttcttcag cttcaggttc gatgcagcca tggtgcggat tgccttgcgc
     2401 cgtggcgcgt tgtcgaggac agccgctccg ccgcgcagcg ataccacgca ccctgtgaag
     2461 cgacagcgcg attttcgtga tcgattcacg cccgcactgt cgggacttct catcgaacct
     2521 tgtcagcacg gcgaccgatg agggtgtcgg cggcctgacg ggcgattgca tcggaacgga
     2581 gcttccctag tcctcggtcc ggctcgaccc gtcgcgatcg cgctcgtggc ggcgtgccac
     2641 gtcgaacggc ggcagccacg cctcgcggcg gatcgtccag agttcgtagg tcggcctgaa
     2701 ccggtcgatg tcgtccagcg cgccgaggt
//
LOCUS       DATCRO010000238         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_137520, whole genome shotgun sequence.
ACCESSION   DATCRO010000238 DATCRO010000000
VERSION     DATCRO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137520"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1574
                     /locus_tag="VIN75_06415"
     CDS             420..1574
                     /locus_tag="VIN75_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973832.1"
                     /translation="MNLSESLALARLRLGAWAWAHGAAARNRPAAPLHFSPGAARRLL
                     LVSRPDAICQSQIYPFHFYRDVLHDRWGYEVREIGLATLLAQPELAPRGADVVCFQAW
                     IDQTPEQQRAIVALLREQHPKARVALLDPCAPTDLRFAAAVGDAVDLYVKKHVLRDLG
                     AYDRPTRGDTNLTQWYGDHFGEPLPETHFALPPGFRSKLVVGPSFVTAPYMLPRFFAV
                     AGAPVGGSRRYDLHARLGGMGSNGWYEKMRTLAFERVRGLEGVSVTPQSFVGKRAYMR
                     ELAHCRMCFSPFGYGEVCWRDYEAVYSGALLIKPDMSHMVTEPDVFVPGETYVPIRWD
                     FADLGEVVAKWLADEPARLRVARNAWDAMHRWARSNAFVDQMERLLDPRP"
     gene            complement(1586..2881)
                     /gene="gshA"
                     /locus_tag="VIN75_06420"
     CDS             complement(1586..2881)
                     /gene="gshA"
                     /locus_tag="VIN75_06420"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY8973833.1"
                     /translation="MVPHLVTALTGPINELEQRVLESMPAIERWFRLEWMEHTPPFYT
                     SVDIRNAGYKLAPVDTNLFPGGFNNLTEPMLPLAIQAAMAAIEKICPEAKNLLLIPEN
                     HTRNSFYLTNVKQLVRIFSSAGLNVRLGSLDPAITEPTEFQLPDGSSLTYEPLVRTPR
                     RLGLKDFDPCTVLLNNDLSAGLPKILENLNEQYLLPPLHAGWAVRRKTNHFHSYEEVA
                     KKFAKLLGMDPWLINPMYSHCGQVDWNEGTGLDCVQTNVDALLTKIRRKHKEYGIAEK
                     PFVVVKADNGTYGMGVMTVRDAKELDEVNRRTRNKMDVVKDGQHVTEVIIQEGVPTYE
                     RVNEAVAEPVVYMIDRYVVGGFYRMHAERGNDENLNAPGASFVPLAFAESSHLPRPGE
                     KPGVSAPNRFYMYGVIGRLAMLAASYELEATDPEAEVYE"
     gene            complement(3022..>3489)
                     /locus_tag="VIN75_06425"
     CDS             complement(3022..>3489)
                     /locus_tag="VIN75_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973834.1"
                     /translation="TYWRSDVRAASIFASVGLRVLFVQGRLGFLRRPWVAPAAFLAGM
                     VLSTHHVPEPLKYSLATLGIALAVNSLEGAPALGRRFLGSRAIGWVGVVSYSLYIWQQ
                     PFYYSIDKYGALPMALAAVAVAVLSFYLVENPVRVRLNALRARRRARLAAAAG"
BASE COUNT          494 a         1206 c         1242 g          547 t
ORIGIN      
        1 gcaggaaggc ggccatgcgg cgcgactcgt cgaccatgtc cttccaggtg aattccttga
       61 gcagcccgcc gcccatgggc tgggtgaaca cggtgcgatc gggcgtcgtg cgttcccagt
      121 ggtacaggcg ctgcagggcg agggaggacg agggcggtgg cgtcatgggg ggcggcggcg
      181 aagtctcgag ccagactagt ggggactcgc tcccggcgcc atggtgaaag cccgcgccgc
      241 gacgtcccgg cggacccccg gccagcgcaa tggcccgcaa ggtcagtccc tgcctcgccg
      301 gggcgggcac gatgcctgct ggcgcacccc tcggccggcg ctcgcgcggg ccgccgcccg
      361 cgccgacctg ttagcgttgc gggcgcgatc ccctttccgc ttccgcttcc gcttcgacga
      421 tgaacctgtc cgaatcactg gccctcgcac gacttcgact gggtgcctgg gcctgggcgc
      481 acggcgccgc cgcccggaac cgcccggcgg ccccgctgca cttctcgccc ggcgcggcgc
      541 gccggctgct gctggtgtcg cggccggacg ccatctgcca gagccagatc tacccgttcc
      601 acttctaccg cgacgtgctg cacgaccgct ggggctacga ggtgcgcgag atcggcctgg
      661 cgaccctgct cgcccagccg gagctggcgc cgcgcggcgc cgacgtcgtg tgcttccagg
      721 cctggatcga ccagacgccc gagcagcagc gcgccatcgt cgccctgctg cgcgaacagc
      781 atcccaaggc gcgcgtcgcg ctgctcgacc cctgcgcccc gaccgacctg cgcttcgctg
      841 ccgccgtcgg cgacgccgtc gacctgtacg tcaagaagca cgtcttgcgc gacctcggcg
      901 cctatgaccg cccgacgcgc ggcgacacca acctgacgca gtggtacggc gatcatttcg
      961 gcgagccgct gcccgagacc cacttcgcgc tgccgcccgg attccgcagc aagctggtgg
     1021 tcggcccgag cttcgtgacg gcgccctaca tgctgccgcg cttcttcgcg gtggcgggcg
     1081 cgccggtggg cggctcgcgc cgctacgacc tgcacgcgcg cctgggcggc atgggcagca
     1141 acggctggta cgagaagatg cgcacgctcg ccttcgagcg cgtgcgcggg ctcgagggcg
     1201 tgtcggtgac gccgcagagc ttcgtcggca agcgcgccta catgcgcgag ctggcgcact
     1261 gccgcatgtg cttcagcccg ttcggctacg gcgaggtctg ctggcgcgac tacgaggccg
     1321 tctactccgg cgcgctgctg atcaagccgg acatgtcgca catggtcacc gagcccgacg
     1381 tcttcgtgcc gggcgagacc tacgtgccga tccgctggga cttcgccgac ctgggcgagg
     1441 tcgtggccaa gtggctggcc gacgagcccg cgcgcctgcg cgtcgcgcgc aacgcatggg
     1501 acgcgatgca ccgctgggcg cgcagcaacg cgttcgtcga ccagatggag cggctgctcg
     1561 acccccgccc ctgagaggca ggggcctact cgtagacctc ggcctcggga tccgtcgcct
     1621 cgagttcgta cgaggccgcc agcatcgcga gccgcccgat cacgccgtac atgtagaagc
     1681 ggttgggcgc gctgacgccc ggcttctcgc ccggcctggg caggtgcgag ctctcggcga
     1741 acgcgagcgg cacgaagctc gcgcccgggg cgttcaggtt ctcgtcgttg ccgcgctcgg
     1801 cgtgcatgcg gtagaagccg ccgacgacgt agcggtcgat catgtagacc accggctcgg
     1861 ccacggcctc gttgacgcgc tcgtaggtcg gcacgccttc ctggatgatg acctcggtga
     1921 cgtgctggcc gtccttgacg acgtccatct tgttgcgcgt gcggcggttg acctcgtcga
     1981 gctccttggc gtcgcgcacc gtcatcacgc ccatgccgta cgtgccgttg tcggccttga
     2041 cgaccacgaa gggcttctcg gcgatgccgt attccttgtg cttgcggcgg atcttggtca
     2101 gcagcgcgtc gacgttggtc tgcacgcagt ccaggcccgt gccctcgttc cagtccacct
     2161 ggccgcaatg cgagtacatc gggttgatca gccacgggtc catgcccagc agcttggcga
     2221 acttcttggc cacttcctcg tagctgtgga agtggttggt cttgcggcgc accgcccagc
     2281 cggcgtgcag cggcggcagc aggtactgct cgttgaggtt ctcgaggatc ttgggcaggc
     2341 cggccgacag gtcgttgttc agcagcaccg tgcaggggtc gaagtccttc aggcccaggc
     2401 ggcgcggcgt gcgcacgagc ggctcgtacg tgagcgagct gccgtcggga agctggaact
     2461 cggtgggctc ggtgatggcc gggtcgagcg agcccaggcg cacgttcagc ccggcgctgg
     2521 agaagatgcg caccagctgc ttgacgttgg tcaggtagaa cgagttgcgc gtgtggttct
     2581 cggggatcag cagcaggttc ttggcctcgg ggcagatctt ctcgatcgcc gccatcgccg
     2641 cctggatcgc cagcggcagc atgggctcgg tgaggttgtt gaagccgccc gggaacaggt
     2701 tggtgtcgac gggcgccagc ttgtagcccg cgttgcggat gtccaccgag gtgtagaagg
     2761 gcggcgtgtg ctccatccac tcgagccgga accagcgttc gatggcgggc atcgattcga
     2821 gcacgcgctg ctcgagttcg ttgatcgggc cggtcaacgc ggtgacgaga tgggggacca
     2881 tgacgggatc ctgggaggct acaagcctga ttctaggcgg acggttcggc tctcccagat
     2941 gggggcacgg ccgggcagca caagggctgc gggccgtgct tttttgcacg caacggcccc
     3001 gacgcgggcc ggccgccagc gtcagccggc ggcggccgcc agccgggccc gccgccgcgc
     3061 ccgcagcgcg ttcaggcgta cgcggaccgg gttctcgacc aggtagaagc tcaggacggc
     3121 gaccgccacg gccgccagcg ccatcggcag cgcgccgtac ttgtcgatcg agtagtagaa
     3181 cggctgctgc cagatgtaga gcgagtacga gacgacgccg acccagccga tggcgcgcga
     3241 gcccaggaag cggcggccga gcgccggcgc gccttccagc gaattgaccg cgagagcgat
     3301 gcccagcgtg gccaggctgt acttcaacgg ctcgggcacg tgatgcgtgc tgagcaccat
     3361 gcccgcgagg aaggccgccg gcgccaccca cggccggcgc aggaagccga gccggccctg
     3421 cacgaacagc acgcgcaggc ccacgctcgc gaagatcgac gcggcgcgca cgtcggagcg
     3481 ccagtaggt
//
LOCUS       DATCRO010000239        15075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1014968, whole genome shotgun sequence.
ACCESSION   DATCRO010000239 DATCRO010000000
VERSION     DATCRO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014968"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VIN75_06430"
     CDS             <1..497
                     /locus_tag="VIN75_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY8973835.1"
                     /translation="IKLEVIGDDHTLQPDPFELVDAAAQLVRLGFQVFPYCTDDLVVC
                     RRLLAAGCEVLMPWAAPIGSGQGVLDPAALRTLRARLPGVPLIVDAGLGAPSHAAFAM
                     ELGYDAVLLNSAIAQAVDPPQMAMAFAQAIAGGRLGYQAGLIAPQDMAIPSTPCANEP
                     ILVE"
     gene            499..2139
                     /gene="thiE"
                     /locus_tag="VIN75_06435"
     CDS             499..2139
                     /gene="thiE"
                     /locus_tag="VIN75_06435"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY8973836.1"
                     /translation="MTANDDTARRPVVWAISGTDSGGGAGLHADIRALDAFGVHGACA
                     VAAVTAQHSTAVTHVEPVRPEVLDAQLAALVDDMPPDAVKTGLLGSLDNLRVVAGWMR
                     RLRERFPERAIPLVVDPVFGATTGAAFADDALRAAYRDELLPLATLATPNVAEARALV
                     PSSQLLGPEALARAWRAFGVRAVAVTGGDSDRLADDEIAQLSTARLASRLAEIDARAA
                     WAYDWLDADHGLRSRGWLASRRIRTGHDHGTGCVFASSAAAALAHGFVAADAVVLAKM
                     ATSEGLRHAYAAGGGAGPVAPRAGFASRLANLPALNPLPHAPSRFAFPGMPEHTRGLY
                     AIVDSGDWVARVLADGVKLVQLRIKDAPPDTLARELRAAIALARAAGATLIVNDHWEL
                     ALELRADGVHLGQEDLAHADIDALHHAGLLLGISTHSYWEVCRARALRPSYIACGPIH
                     ATAIKQMPWIPQGDANLAYWCALLSDTPVVAIGGMDAARARDAMRAGAWGVAVVGAIT
                     RSDDPHAALLALRDATAPTPADARPPSFARSTLATSAC"
     gene            2388..4022
                     /locus_tag="VIN75_06440"
     CDS             2388..4022
                     /locus_tag="VIN75_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8973837.1"
                     /translation="MRPSDPTIQMPPAALAPAWKGVAVGVLRVLWLALLVLPATRSVP
                     ATEAWAVDRQLLAGLACWLVLATRIILPARWFFPLTLPLALAASWWAGAALLRHVDLL
                     DLASQWRTFRPEEVRAALAPYVVPAVLGGLVVASWCVACARAADAWRRRRALAVGAAL
                     LTLALAPLAPPVAWQRAWPTNALLLAITGTARATSLTGLRPPPQFTPRDPKATWHARA
                     TSKAARQTLVFIIGESIRADYLRECHGPERIRALGDGAIVACDVTAGADATHTSVPLL
                     VSREMPGWAHLVSRDATFAHAFDEAGFETHWISVQAINLAWPDARFQDYPQVVGTDEA
                     VLGPPLAAALARPAAHKAIVLHAVNAHEPYCKRYDPAQAPYPADCRGPAIMPSIASDP
                     ANVRATYANAVDATVRFIDGVIDQLRTQRGEVFLLFTPDHADNLYDDRRQLRGHALTN
                     PTRWDIRVPAIFWANDAWRAAHPAQWANLQAQAAAPLMHADMVPTFLAAGGVAYDEPR
                     KLAVDLLDATVPPRRRLVQRELGATTDWDTLVREAR"
     gene            4102..4920
                     /locus_tag="VIN75_06445"
     CDS             4102..4920
                     /locus_tag="VIN75_06445"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8973838.1"
                     /translation="MDWSNFKHILDAYDFPLFTLGKTEITLFTLLKLAISGLVLWFLA
                     GRFSRWTLKRLLGRTNMQEGQRLAIASLVHYVVLVFGAVVILQNAGIELTAFAIVGSA
                     LGVGVGFGLQNIISNFISGLIVLLERPIEIGDRIEVSGVEGVVREIGARRTTVVTPDD
                     IAILVPNQSFITSNVTNYVYLKKCVRIRVPVPIAPGQDIRRVEGLLLEATKLGPVLPD
                     PAPQVAITAVTASAVTLEVWAWYEARRYAKSEVLSQVYFHVIDSLARNEVKLAG"
     gene            complement(4943..6985)
                     /locus_tag="VIN75_06450"
     CDS             complement(4943..6985)
                     /locus_tag="VIN75_06450"
                     /inference="COORDINATES: protein motif:HMM:NF021945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HEY8973839.1"
                     /translation="MNSRHLLAPAAGFPRALAFPLSVVTAALLAACGGGNGQSSTTVS
                     PESAGTAADTAAAMATAAAASPVCFYEHVNYQGASFCATADSSWVGGTWNDRISSVKV
                     AAGYKVTLYWDINYGGKTLVLNGDTANLVALGFNDQASSYRIVAPAPATSKVGTIQIA
                     QSQLFNSDDPALVLVASKPALLKVNAVATTAGAARPVVRLHIDNVNGGASSDVLMTAP
                     TAALPTAVPAVPSFADSYTATVPADAVRSGLRLSVQLDSAPATTLTPKVGGGVPMKFV
                     PISINIAGTAGQRPTDLAPHLQSLFPVSSVVTQQHPTYTSTKVTTFPADENAWSDAYG
                     KVLGELADLHSLEGAGSQDHYFGFMPKRTWGLAGLGYIGGSAAVGFDMPSAPTSVRDV
                     ASHELGHNFSLQHAACGGAGNPDPNYPYPDADLSKPGRVVWPYLADEKSFYDPRPTDR
                     HDIMSYCGGQVFSDYNYTVMQSWLYPGDKTLKASVQSVAAPPSTPAAEPREVLLVSGE
                     IGASATTLNPVKSFVGRPDAAASGAYVLRVVTAAGTADYPFTPLQLDHDARTQHFSVA
                     IPNTGAVQSVTVLHQGRTLVQRAAGSERATVQSAHEAVPLQVREAAGQAIVTWDARAN
                     AFATVTWVGPTGQRLTLAQDLRGGSAQLSTAELPAGGSFEVILSDGLNSTRQSVRR"
     gene            7437..8336
                     /locus_tag="VIN75_06455"
     CDS             7437..8336
                     /locus_tag="VIN75_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8973840.1"
                     /translation="MTRPLRIEFPGAVYHVTSRGDRQEPIFRDDNDRQRLLDVIDHAM
                     GRLDAEMLAFCLMGNHYHFVLRTRRPNLSRLMRHINGEYTQAFNRRHAVSGHVFQGRF
                     HAVIVDTDAYLLEVCRYVELNPVRAGLVSTVDGWPWSSYRAHVGMARGPTWLATAELH
                     SHLLGRYPTNPDDRRQARDLYANTVAAGRDANPWKLHLRQEIYLGEEAFVADTRARAT
                     AHRLRSPEIARTQRTSQNTLAEWLTPERTRDEAFRLAYSLGGMTMAEIARQAGMSVSG
                     VSRIIAIAERLHESRPDPAIVKT"
     gene            complement(8326..10104)
                     /locus_tag="VIN75_06460"
     CDS             complement(8326..10104)
                     /locus_tag="VIN75_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY8973841.1"
                     /translation="MIDFRSPDEGVAPLRLAFGAPRESLRATSLDDVAPLLARVDALA
                     RAGAWCVGYLCYEAAGAFDEAFETHAPADPARPLAAFAVYDAPLPDAAAFESDAAATV
                     QWTGGPQRDAFDAVIAEILRAIADGEVYQVNATAPLTGTLQGDALALFAALRRAQPNA
                     YAAYLDLGTANEPDDRILSVSPELFFDWRDDRLLARPMKGTAPRGATPREDAAQAQFL
                     RTAAKERAENLMIVDLLRNDLSRLARPHSVRVPRLFHTEAWPTVWQMSSDVAAATRPG
                     TTLEQVFGALFPCGSITGAPKVQAMRLIRQLEPEPRGIYCGAIGVVQPGGAATFNVPI
                     RTLALRCLEKDGVATTHVRCGIGSGITADATAAGEWDEWRHKRAFVDRAGQAFELLET
                     LRLEEGVFVDVEAHLARMAEAAAHFAFASPVDSAREALDALRLVHPTGRRRVRLLASR
                     AGVAHAQAFALADTPTPVRVRVAERPLAGSTGEFVRFKTTQRAHYDAFAPAGDDVFDT
                     LLWNERGELTEFTRGNVALRIDGRWLTPALRCGLLPGIARARLLREGAIEEAVLVRDD
                     LARADGLAFFNSLRGWLEATQGGVRS"
     gene            complement(10180..10947)
                     /gene="fliR"
                     /locus_tag="VIN75_06465"
     CDS             complement(10180..10947)
                     /gene="fliR"
                     /locus_tag="VIN75_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY8973842.1"
                     /translation="MISLSEAQIMAWLVPVLWPFLRVLALFSSMPVLGQRNVPIRVRI
                     ALAFFIAIGVAGTAAPTAAANVPLDSPAAILLVVQQVLIGLSLGFAVRIIFTAIEFAG
                     EVIGLQMGLNFAGFFDPATAQQATATSSLFSTMVAFLFIAMNGHLLVIDAVVQSLVAF
                     PVGPEPFAFLHAVRPETWGAEIFRLGLWIALPLIAMLLFVNMMLGIIARVAPQISIFS
                     VGFPITLGVGLVGVMFTLPLLQGPFTAALEQMLMMFR"
     gene            complement(11057..11326)
                     /gene="fliQ"
                     /locus_tag="VIN75_06470"
     CDS             complement(11057..11326)
                     /gene="fliQ"
                     /locus_tag="VIN75_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY8973843.1"
                     /translation="MDAAQVFTVGQQALYTLLTVAAPVLLVVLVVGILVSIFQAATSI
                     NEATLTFVPKLLAAVAVLGVAGPWMLTSLVEYLRSVLLSLPSAAS"
     gene            complement(11415..12173)
                     /gene="fliP"
                     /locus_tag="VIN75_06475"
     CDS             complement(11415..12173)
                     /gene="fliP"
                     /locus_tag="VIN75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059128.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY8973844.1"
                     /translation="MRKFAKLAAVAALALPVLAIAQSAPAANGPSLPLLVGASGGGTN
                     FSVPIQTLLFFTALTFLPAILLMMTGFTRIVIVLSLFRQAIGTQAAPPNQVITGLSLF
                     LTFFVMSPTIDKVYTQAWAPYQANQISFNDALDKGQAPMREFMLKQTRQSDVQLFAKL
                     AKLPADVKGDAVPFKVLVPAFVASELKTAFQIGFLIFIPFLIIDMIVASVLMSLGMMM
                     LSPVLVALPFKLMLFVLADGWNLLLGSLAASFAT"
     gene            complement(12166..12534)
                     /locus_tag="VIN75_06480"
     CDS             complement(12166..12534)
                     /locus_tag="VIN75_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY8973845.1"
                     /translation="MTTSLMPLVAFLFVIAMIPVALWLMKRAGLGGAGGPQGSVLRHV
                     SQLSLGTSQRVTVVEIAVGVERHWLVLGVTGERVTQLASYPAPDITAAPSAPAHTAAV
                     NQLIARWRGATGSQEGSRDA"
     gene            complement(12610..13047)
                     /gene="fliN"
                     /locus_tag="VIN75_06485"
     CDS             complement(12610..13047)
                     /gene="fliN"
                     /locus_tag="VIN75_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY8973846.1"
                     /translation="MAAEEMSDDDAMAAEWAAALAESKSEPAGGMSGEIQTETVAPAN
                     FTNFASTEGISGAGNDLNMILDIPVQLTVELGRTRIPIKHILQLAQGSVVELDALAGE
                     PMDVLVNGFLIAQGEVVVVNDKFGIRLTDIVTPSERMRRLSRT"
     gene            complement(13037..14041)
                     /gene="fliM"
                     /locus_tag="VIN75_06490"
     CDS             complement(13037..14041)
                     /gene="fliM"
                     /locus_tag="VIN75_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY8973847.1"
                     /translation="MNQQILSQDEVDALLQGITGESQKLEQEEQVEGSIRNYDLANQE
                     RIVRGRMPTMEIIGERFARNIRIGLFNFIRKSPEVSINGIKVQKYSAFLREIVVPTNF
                     NIVSVRPLRGSGLIVCDPSLVFAVIDALFGGVGKFHTRIEGRDFSPTEQRVIQRLVDC
                     IITEYRNAWQGIYPLDLEYQRSEMQPQFANIATPSEIVVASSFTLEVGETSGSVHFCI
                     PYSTLEPIRDVLYSTIQGDSVEPDRRWVNLLKAQIQSAEVDLVAELGTAPATVEQLLS
                     FKPGDFVELDLDPMIQAKVDGVPVFDCHYGTSAGKYAIKIDRLLTSSNLSWLGDGNGS
                     "
     gene            complement(14075..14707)
                     /locus_tag="VIN75_06495"
     CDS             complement(14075..14707)
                     /locus_tag="VIN75_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY8973848.1"
                     /translation="MSAAAAAADAVPAKSGGKKKLVLMVAVAALVLGGGGVGALVYMK
                     KQKAAAEAAAAEDGDAADTAPAKHEEKKKKGEKPVFVPMDAFVVNLADHDADRYAQIG
                     IVLEVPDEHASEDIKTYLPAIRNNILLLLAHKSSADLAGGDGKELLARQIRREALKAM
                     GEDVDEDDDAAPVADGASAAAPAKKKKKKKAAENDSAPIRSVQFSSFIIQ"
BASE COUNT         2211 a         5319 c         5341 g         2204 t
ORIGIN      
        1 ggatcaagct ggaggtgatc ggcgacgacc atacgctgca gcccgacccg ttcgagctgg
       61 tcgacgcggc cgcgcagctg gtcaggctcg gcttccaggt gttcccgtac tgcaccgacg
      121 acctcgtggt gtgccgccgc ctgctggccg ccggctgcga ggtgctgatg ccgtgggccg
      181 cgccgatcgg ctcgggccag ggcgtgctgg acccggccgc gctgcgcacg ctgcgcgcgc
      241 ggctgccggg cgtgccgctg atcgtcgacg ccggcctcgg cgcgccgtcg cacgccgcgt
      301 tcgcgatgga gctcggctac gacgcggtgc tgctgaactc ggcgatcgcg caggccgtcg
      361 acccgccgca gatggcgatg gccttcgcgc aggcgatcgc cggcggccgc ctgggctacc
      421 aggccggcct catcgcgccg caggacatgg cgatccccag cacgccgtgc gccaacgaac
      481 ccatcctggt cgagtgagat gacggcgaac gacgacaccg cgcggcgccc cgtcgtgtgg
      541 gcgatctcgg gcaccgactc cggcggcggc gcgggcctgc acgcggacat ccgcgcgctc
      601 gacgcgttcg gcgtgcacgg cgcctgcgcg gtggccgcgg tgacggcgca gcactcgacg
      661 gccgtcacgc acgtcgagcc ggtacggccc gaggtcctgg acgcgcagct cgcggcgctg
      721 gtcgacgaca tgccgcccga tgcggtcaag accggcctgc tgggcagcct cgacaacctg
      781 cgggtcgtcg cgggctggat gcggcgcctg cgcgagcgct tccccgagcg cgcgatcccg
      841 ctggtcgtcg atcccgtgtt cggcgcgacc accggcgcgg cgttcgccga cgacgcgctg
      901 cgcgccgcct accgcgacga actgctgccg ctggccacgc tggccacgcc caacgtggcc
      961 gaggcgcgcg cgctggtgcc gtcgtcgcaa ctgctgggcc ccgaggcgct cgcccgcgcc
     1021 tggcgcgcgt tcggcgtgcg cgcggtggcc gtcacgggcg gcgattccga tcgcctggcc
     1081 gacgacgaga tcgcgcagct gtcgacggcc aggctcgcct cgcgcctggc cgagatcgac
     1141 gcccgcgccg cgtgggcgta cgactggctc gacgccgacc acggcctgcg cagccgcggc
     1201 tggctggcct cgcgccgcat ccgcaccggg cacgaccacg gcaccggctg cgtgttcgcg
     1261 tcgtccgccg cggccgcact ggcgcacggc ttcgtcgcgg cggacgcggt ggtgctggcc
     1321 aagatggcca cgagcgaggg cctgcgccac gcgtacgcgg ccggcggcgg cgccggcccc
     1381 gtggcgccgc gcgcgggctt cgcgagccgg ctcgccaacc tgcccgccct caacccgctg
     1441 ccgcacgcac cctcgcgctt cgcgttcccc ggcatgccgg agcacacgcg cggcctgtac
     1501 gccatcgtcg actccggcga ctgggtggcg cgcgtgctgg ccgacggcgt caagctggtg
     1561 cagctgcgca tcaaggacgc gccgcccgac acgctggcgc gcgaactgcg cgccgccatc
     1621 gccctcgcgc gcgccgccgg cgccacgctg atcgtcaacg accactggga gctcgcgctg
     1681 gaactgcgcg ccgacggcgt gcacctgggc caggaagacc tggctcacgc cgacatcgat
     1741 gcgctgcacc acgccggcct gctgctgggc atcagcacgc actcgtactg ggaggtctgc
     1801 cgcgcccgcg cgctgcgccc cagctacatc gcgtgcgggc cgatccacgc caccgccatc
     1861 aagcagatgc cgtggatccc gcaaggcgac gccaacctcg catactggtg cgcgctgctg
     1921 agcgacacgc ccgtggtggc catcggcggc atggacgccg cgcgtgcgcg cgacgcgatg
     1981 cgcgccggcg cctggggcgt ggcagtggtg ggcgccatca cgcgcagcga cgatccgcac
     2041 gccgcgctgc tcgccctgcg cgacgcgacg gcgccgacac cggccgacgc acggccgccg
     2101 tcgttcgcgc gcagcacgct ggcgacgagc gcctgctgag gcgagcggtc gcgacactgc
     2161 gctgacgtcc ctcgggtgcg cgccaccttg gcctgtcatc ctgcgcgcag ccgcaggatc
     2221 gacgcgcgcc aaggcactgc ccggccgagc tggacctgcg actgcgcctg gctccgcgca
     2281 ggcggacagc gtcgcggccc gggccggctc actacaatgg cccgcgcctg ccccgcaggt
     2341 cgggcacccg cggatctgcg gcatcctgct tcccctccgc ccttccgatg cgcccgtccg
     2401 acccgaccat ccagatgcct cccgccgccc tcgcgccggc ctggaagggc gtcgcggtcg
     2461 gcgtgctgcg cgtgctgtgg ctcgcgctgc tggtcctgcc ggccacgcga tcggtgccgg
     2521 ccaccgaggc ctgggccgtc gaccggcagc tgctggcggg cctcgcgtgc tggctggtgc
     2581 tggccacgcg catcatcctg cccgcgcgct ggttcttccc gctgacgctg ccgctggcgc
     2641 tggccgccag ctggtgggcg ggtgccgccc tcctgcgcca cgtcgacctg ctggacctcg
     2701 cctcgcaatg gcgcacgttc cggcccgagg aggtgcgggc cgcgctcgcg ccctacgtcg
     2761 tgccggcggt gctgggcgga ctcgtggtcg cgagctggtg cgtggcgtgc gcccgcgcgg
     2821 cggacgcgtg gcgcaggcgc cgcgcgctgg ccgtcggcgc cgcgctgctg accctcgcgc
     2881 tggcgccgct ggcgccgccg gtcgcgtggc agcgcgcctg gccgaccaac gcgctgctgc
     2941 tggccatcac gggcaccgcg cgcgccacgt cgttgaccgg gctgcgacca ccgccccagt
     3001 tcacgccgcg cgatccgaag gccacctggc acgcccgcgc gacgagcaag gccgcgcgcc
     3061 agacgctggt cttcatcatc ggcgagagca tccgcgccga ctacctgcgc gagtgccacg
     3121 gccccgagcg catccgcgcc ctgggcgacg gcgcgatcgt cgcctgcgac gtcaccgccg
     3181 gcgcggatgc gacgcacacc tcggtgccgc tgctcgtctc gcgcgagatg cccggctggg
     3241 cccacctcgt ctcgcgggac gcgaccttcg cgcacgcgtt cgacgaggcc ggcttcgaga
     3301 cgcactggat cagcgtccag gccatcaacc tcgcgtggcc cgacgcccgc ttccaggact
     3361 atccgcaggt cgtcggcacc gacgaggcgg tgctgggccc gccgctggcg gccgccctcg
     3421 cgcgcccggc ggcccacaag gcgatcgtgc tgcacgcggt caacgcccac gagccctact
     3481 gcaagcgcta cgaccccgcg caggcgccct atccggccga ctgccggggc ccggccatca
     3541 tgccctcgat cgcctcggac ccggcgaacg tgcgcgcgac ctacgcgaac gccgtcgacg
     3601 ccacggtgcg cttcatcgac ggcgtcatcg accagctgcg cacccagcgc ggcgaggtct
     3661 tcctgctgtt cacacccgac cacgcggaca acctgtacga cgacaggcgc cagctgcgcg
     3721 gccacgcgtt gaccaacccc acgcgttggg acatccgcgt gccggcgatc ttctgggcca
     3781 acgacgcgtg gcgcgccgcg caccccgcgc aatgggccaa cctgcaggcg caggccgcgg
     3841 cgccgctgat gcacgccgac atggtgccga ccttcctggc cgccggcggc gtggcctacg
     3901 acgagccgcg caagctggcg gtcgacctgc tggacgccac cgtcccgcca cgccggcgcc
     3961 tcgtccagcg cgagctcggc gcgaccacgg attgggatac gctcgtgcgc gaggcgcgct
     4021 gagcgcaggc gcgccgacgc gacggcggcg cctcgcacgc cgattgccgc tgggtcggcc
     4081 ttgccgagac gggagccctt cgtggactgg tccaacttca agcacatcct cgacgcctac
     4141 gacttcccgc tgttcacgct cggcaagacc gagatcacgc tgttcacgct gctgaagctg
     4201 gccatctcgg gcctggtgct gtggttcctg gccggccgct tcagccgctg gacgctcaag
     4261 cgcctgctgg gccgcacgaa catgcaggag ggccagcgac tggccatcgc gagcctcgtg
     4321 cactacgtcg tgctggtgtt cggcgcggtg gtgatcctgc agaacgcggg catcgagctg
     4381 accgccttcg ccatcgtcgg cagcgcgctc ggcgtgggcg tcggcttcgg cctgcagaac
     4441 atcatcagca acttcatcag cggcctgatc gtgctgctgg agcggcccat cgagatcggc
     4501 gaccgcatcg aggtctccgg cgtcgagggc gtggtgcgcg agatcggcgc ccggcgcacg
     4561 acggtggtca cgcccgacga catcgcgatc ctggtgccga accagagctt catcacgagc
     4621 aacgtgacga actacgtcta cctcaagaag tgcgtccgca tccgcgtgcc ggtgcccatc
     4681 gcgcccgggc aggacatccg gcgcgtcgag ggcctgctgc tggaggccac gaagctcggc
     4741 cccgtgctgc ccgacccggc gccgcaggtg gcgatcaccg cggtgacggc gtcggcggtg
     4801 accctggagg tctgggcctg gtacgaggca cgccgctacg cgaagtcgga agtcctgagc
     4861 caggtctact tccacgtcat cgactcgctc gcacgcaacg aggtgaagct ggcgggctga
     4921 gacccgccgg cgcccggccc gatcagcggc gcaccgactg gcgcgtcgag ttcaggccgt
     4981 cggacaggat cacctcgaac gagccgcccg cgggcagttc ggcggtggac agctgcgcgc
     5041 tgccgccgcg aaggtcctgc gccagtgtca ggcgctggcc ggtcgggccg acccaggtga
     5101 cggtcgcgaa cgcgttggcg cgcgcgtccc acgtgacgat cgcctggccc gcggcttccc
     5161 tgacctgcaa cggtaccgcc tcgtgcgcgg actgcaccgt ggcgcgttcg ctgcccgccg
     5221 cgcgctgcac cagcgtcctg ccctggtgaa gcacggtgac gctctgcacg gcgcccgtgt
     5281 tcgggatcgc gacgctgaag tgctgcgtcc tggcgtcgtg gtccagctgc agcggcgtga
     5341 acggatagtc ggcggtgccg gcggccgtga ccacgcgcag gacgtaggcg cccgaggccg
     5401 ccgcgtccgg gcggccgacg aaggacttga ccgggttcaa cgtcgtcgcg ctcgcgccga
     5461 tctcgccgct gaccagcagc acctcgcgcg gctcggcagc gggcgtcgac ggcggggccg
     5521 ccaccgactg caccgaggcc ttgagcgtct tgtcgccggg gtagagccac gattgcatga
     5581 cggtgtagtt gtagtcggag aacacctggc cgccgcagta gctcatgatg tcgtggcggt
     5641 cggtcgggcg cgggtcgtag aagctcttct cgtcggccag gtagggccac acgacgcggc
     5701 cgggcttgct caggtcggcg tccggatacg ggtagttcgg gtcggggttg ccggcgccgc
     5761 cgcaggccgc gtgctgcagc gagaagttgt ggcccagctc gtgcgaggcc acgtcgcgca
     5821 ccgaggtggg cgcgctgggc atgtcgaagc ccacggcggc gctgccgccg atgtagccca
     5881 ggccggccag gccccaggtg cgcttgggca tgaagccgaa gtagtggtcc tgcgagccgg
     5941 cgccttccag cgaatgcagg tcagccagtt cgcccaggac cttgccgtac gcgtcgctcc
     6001 atgcgttctc gtcggccggg aacgtggtga ccttggtcga ggtgtaggtc ggatgctgct
     6061 gcgtcaccac gctggagacc gggaacagcg actgcaggtg cggcgcgagg tcggtcgggc
     6121 gctggcccgc ggtgccggcg atgttgatcg agatcggcac gaacttcatc ggcacgccgc
     6181 cgcccacctt gggcgtcagc gtggtggcgg gcgcggaatc gagctgcacg gacaggcgca
     6241 ggcccgacct gacggcgtcg gcgggaaccg tggcggtgta cgagtcggcg aagctcggca
     6301 cggcgggcac cgcggtgggc agggccgcgg tcggcgcggt catcagcacg tcgctggagg
     6361 cgccgccgtt gacgttgtcg atgtgcaggc gcacgaccgg cctggccgcg ccggccgtgg
     6421 tggccacggc gttgaccttc agcagcgccg gcttgctcgc gaccagcacg agcgccgggt
     6481 cgtcgctgtt gaacagctgc gactgcgcga tctggatcgt gcccaccttg gacgtggccg
     6541 gcgccggcgc gacgatcctg tacgacgagg cctggtcgtt gaagcccagc gccacgaggt
     6601 tggccgtgtc accgttcagc accagggtct tgccgccgta gttgatgtcc cagtacaggg
     6661 tgaccttgta gcccgccgcg accttcaccg aggagatgcg gtcgttccag gtgccgccga
     6721 cccagctgct gtcggccgtc gcgcagaagc tcgcgccctg gtagttgacg tgctcgtaga
     6781 aacacacggg cgacgcggcc gcggcggtgg ccatcgcggc ggccgtgtcg gctgcggtgc
     6841 cggccgactc cggcgacacc gtggtcgaag actggccgtt cccgccgccg caggccgcga
     6901 gcagtgctgc cgtgacgacg gacagcggga acgccagcgc gcgcgggaag ccggcggccg
     6961 gggcgaggag gtggcgtgag ttcaaggcag gtctccacaa gggttgttgg caccgactgg
     7021 agacagcgcg gccgtccgga tcgggggctc gatcgtcggg gccagcgcga gccgcggcgt
     7081 aacccggtgc tcgcgcttgt aaaggtttgg taaccgaaac atcgagggcc ggccgccatt
     7141 ttctcgaaag cgctttcata tccggtagcg ataccgcccc agggtatccg gccgcgcatt
     7201 gcggcccgat aacggcctga acgcccgcgg cggcgcgatc acgtctcgtc acggatttga
     7261 gaggggatcc cccaagaact gggggctagc gccaagcgcc aatcggacgg ggtcaggtct
     7321 tgaacgttgc ggggtcaggt cttgaatcgt gcatttccga cgttccgcgc attcaggccg
     7381 acatacgcag cgattggcgc agcgggcatc caccgtccgg cccctaagct cgaccgatga
     7441 cacgacctct tcgaatcgaa tttcccggtg ccgtctacca cgtcacttcc cgaggcgatc
     7501 ggcaagagcc gatcttccgg gacgacaacg acaggcaacg ccttctcgac gtcatcgacc
     7561 acgcgatggg acgcctggac gccgagatgc tggcgttctg cctcatgggc aaccactacc
     7621 acttcgtgct gcgcacacgt cgaccgaacc tttctcgcct gatgcggcac atcaacggcg
     7681 aatacacgca ggctttcaac aggcgccatg cagtgagcgg acacgtattc cagggacgct
     7741 tccatgccgt catcgtcgac acggatgcgt atttgctgga ggtctgccgc tatgtcgaac
     7801 tgaatccagt gcgagcagga ttggtgagca cggtcgacgg ctggccgtgg agcagctatc
     7861 gcgcccatgt cggcatggcg cgcggaccga catggctggc gaccgcggaa ctgcacagcc
     7921 acctgctggg tcggtacccc acgaatccag acgatcgccg tcaggcccgg gatctgtatg
     7981 cgaacaccgt cgcggccggg cgcgacgcaa atccgtggaa gttgcatcta cgccaggaaa
     8041 tctatctggg cgaagaagcg ttcgtcgccg atacccgggc gcgagccacc gcgcatcgct
     8101 tgcgcagtcc ggagatcgcg aggactcagc ggacctcgca gaacacgctg gctgaatggc
     8161 tgactcctga gcgaacgcgg gacgaggcct tccgtctcgc ctactcgctg ggcggcatga
     8221 ccatggcgga aatcgcccgg caggccggca tgtccgtttc gggtgtcagc cgcatcatcg
     8281 cgatcgccga gagactgcac gaatcaagac ctgaccccgc aatagtcaag acctgacccc
     8341 accctgagtc gcctccagcc acccgcgcag gctgttgaag aacgccagtc cgtcggcccg
     8401 cgcgaggtcg tcgcgcacca gcaccgcctc ctcgatcgcc ccttcgcgca gcaggcgggc
     8461 gcgggcgatg ccggggagca agccgcagcg caaagcgggc gtcagccagc ggccgtcgat
     8521 gcgcagcgcg acattgccgc gcgtgaactc ggtcagctcg ccgcgctcgt tccacagcaa
     8581 ggtgtcgaag acgtcgtcgc cggcgggcgc gaacgcgtcg tagtgggcgc gctgcgtcgt
     8641 cttgaagcgc acgaactcgc cggtgctgcc cgccagcggg cgctcggcga cgcgcacccg
     8701 cacgggggtc ggcgtgtccg cgagcgcgaa ggcctgcgcg tgcgcgacgc ccgcgcgcga
     8761 cgcgagcagg cgcacgcgcc ggcgcccggt cgggtgcacg aggcgcaatg catcgagcgc
     8821 ctcacgcgcg gagtcgaccg gcgatgcgaa cgcgaagtgc gccgcggcct cggccatgcg
     8881 cgccaggtgc gcctcgacgt cgacgaagac cccctcctcc agccgcagcg tctccagcag
     8941 ctcgaaggcc tggcccgcgc ggtcgacgaa ggcgcgcttg tggcgccact cgtcccactc
     9001 gccggcggcg gtggcgtcgg ccgtgatgcc gctgccgatg ccgcagcgca cgtgcgtggt
     9061 cgcgacgccg tccttctcga ggcagcgcaa cgccaacgtg cggatcggca cgttgaacgt
     9121 ggcggcgccg ccgggttgca ccacgccgat cgcgccgcag tagatgccgc gcggctcggg
     9181 ctcgagctgc cggatcagcc gcatcgcctg caccttgggc gcgcccgtga tcgagccgca
     9241 cgggaacagc gcgccgaaca cctgctccag cgtcgtgccg gggcgcgtcg cggcggccac
     9301 gtcggaactc atctgccaca ccgtgggcca ggcctcggtg tggaacaggc gcggcacgcg
     9361 caccgaatgc ggcctggcca ggcgcgacag gtcgttgcgc agcaggtcga cgatcatgag
     9421 gttctcggcg cgctccttgg ccgcggtgcg caggaactgc gcctgcgcgg cgtcctcccg
     9481 cggcgtcgcg ccgcgcggcg cggtgccctt catcggacgc gccagcaggc ggtcgtcgcg
     9541 ccagtcgaag aacagctccg gcgagacgga caggatgcgg tcgtccggct cgttcgcggt
     9601 gcccagatcg aggtaagccg cgtacgcgtt gggctgcgcg cgccgcagcg cggcgaacaa
     9661 cgcgagcgcg tcgccctgca gcgtgccggt cagcggcgcg gtggcgttga cctggtagac
     9721 ctcgccgtcc gcgatcgccc gcaggatctc ggcgatcacc gcgtcgaacg catcgcgctg
     9781 cgggccgccc gtccactgca cggtggcggc cgcgtcgctc tcgaaggcgg ccgcatccgg
     9841 caagggtgcg tcgtacacgg cgaacgccgc gagcgggcgc gcgggatcgg ccggtgcatg
     9901 cgtctcgaag gcctcgtcga aggcgcccgc ggcctcgtag cagagatagc ccacgcacca
     9961 ggcccccgcg cgcgccagcg cgtccacgcg cgcgagcagc ggcgccacgt cgtccaggct
    10021 cgtcgcgcgc agcgattcgc gcggagcgcc gaacgcgagg cgcagcggcg cgacgccctc
    10081 gtcgggcgag cggaagtcga tcatggcggt gggacggatc ccgggtgggg acatgcgcgg
    10141 acgtgctcgt ggcagaccgc cgattttagc gaggcgtgct cagcgaaaca tcatgagcat
    10201 ctgctcgagg gcggccgtga acggcccctg cagcagcggc agcgtgaaca tcacgccgac
    10261 caggccgacg cccagcgtga tcgggaaacc caccgagaag atgctgatct gcggcgccac
    10321 gcgcgcgatg atgcccagca tcatgttgac gaacagcagc atggcgatca gcggcagcgc
    10381 gatccacagc cccaggcgga agatctcggc gccccaggtc tcgggccgca cggcgtgcag
    10441 gaacgcgaac ggctcggggc ccacggggaa ggcgaccagg ctctggacga cggcgtcgat
    10501 caccagcagg tggccgttca tcgcgatgaa caggaacgcc accatcgtgg agaacaggct
    10561 gctcgtggcg gtggcctgct gcgcggtggc ggggtcgaag aagccggcga agttcaggcc
    10621 catctgcagg ccgatgacct cgcccgcgaa ctcgatggcg gtgaagatga tgcgcaccgc
    10681 gaagcccagc gacaggccga tcagcacctg ctgcaccacc agcaggatcg cggcgggcga
    10741 atccagcggc acgttggcgg cggccgtcgg ggccgcggtg ccggcgacgc cgatcgcgat
    10801 gaagaacgcg agcgcgatgc gcacgcggat cggcacgttg cgctggccca gcacgggcat
    10861 cgaggagaac agcgccagca cgcggaggaa cggccacagc accggcacca gccaggccat
    10921 gatctgggct tcgctgagcg agatcatgtc ggagcgtcga tcaggtgttc gaaggatgcg
    10981 gccgacgtcg cgctcgcccg ctcccggagc aggcggccgc gcccctcggg ggctgcgagc
    11041 gacgcgagcg gggccgtcat gacgccgcgg acggcagcga cagcagcacg ctgcgcaggt
    11101 actcgaccag cgaggtgagc atccacgggc cggccacgcc cagcaccgcg acggcggcca
    11161 gcagcttggg cacgaaggtc agcgtcgcct cgttgatgga cgtcgcggcc tggaagatgc
    11221 tcaccaggat gccgacgacc agtaccacca gcagcaccgg ggcggccacg gtcagcaggg
    11281 tgtagagggc ttgctgaccg acggtgaaga cttgtgcggc gtccatgggg aatgcaattc
    11341 ctgcgttggg atggtgacgt catgttgccg gcgcgcagcc ggcgcgagag ggcgataagc
    11401 ggggcgtttc ggacctacgt cgcgaagctt gccgcgagcg accccagcag caggttccac
    11461 ccgtccgcca gcacgaacag catcagcttg aacggcagcg ccacgagcac cggcgacagc
    11521 atcatcatgc ccagcgacat cagcacgctg gccacgatca tgtcgatgat caggaacggg
    11581 atgaagatca ggaagccgat ctggaacgcc gtcttgagtt cgctggccac gaaggccggc
    11641 accagcacct tgaacggcac cgcgtcgccc ttgacgtcgg ccggcagctt ggccagcttg
    11701 gcgaacagct gcacgtcgga ctggcgcgtc tgcttgagca tgaactcgcg catcggcgcc
    11761 tggcccttgt cgagcgcgtc gttgaagctg atctggttgg cctggtacgg cgcccaggcc
    11821 tgcgtgtaga ccttgtcgat cgtcggggac atcacgaaga aggtcaggaa cagcgacagg
    11881 ccggtgatca cctggttggg cggcgcggcc tgcgtgccga tcgcctggcg gaacagcgag
    11941 agcacgatca cgatgcgggt gaagcccgtc atcatcagca ggatcgcggg caggaaggtc
    12001 agcgccgtga agaacagcag cgtctggatc ggcaccgaga agttggtacc gccgccgctg
    12061 gcgcccacca gcaggggcag gctcgggccg ttggcggccg gcgcggactg cgcgatcgcg
    12121 aggacgggca gcgcgagcgc ggcgaccgcg gcgagcttgg cgaacttacg catcgcgcga
    12181 gccctcctgc gagccggtgg cgccgcgcca gcgggcgatc agctggttga cggccgcggt
    12241 gtgcgcgggc gcggacggcg ccgcggtgat gtcgggggcg gggtagctcg cgagctgcgt
    12301 cacgcgctcg ccggtcacgc ccagcacgag ccagtggcgc tcgacgccga cggcgatctc
    12361 gaccaccgtc acgcgctgcg acgtgcccag gctcagctgc gacacgtgcc gcagcacgct
    12421 gccctgcggg ccgccggcgc cgccgaggcc ggcgcgcttc atcaaccaca gcgcgacggg
    12481 gatcatcgcg atcacgaaca ggaatgccac gaggggcatg agggacgtcg tcatggtgtt
    12541 tctctttgtc gaccggcgca tcgaggcgcg gaagcgtcgg cggccggaac cgccaggggg
    12601 ctgccaggat caggtgcggc tcaggcggcg catgcgctcg ctgggcgtca cgatgtcggt
    12661 caggcggatg ccgaacttgt cgttgacgac caccacctcg ccctgcgcga tcaggaagcc
    12721 gttgaccagc acgtccatcg gctcgccggc cagcgcgtcg agttcgacga ccgagccctg
    12781 cgcgagctgc aggatgtgct tgatcgggat gcgggtgcgg cccagctcca cggtcagctg
    12841 caccgggatg tccaggatca tgttgaggtc gttgccggcc ccgctgatcc cttcggtcga
    12901 tgcgaagttg gtgaagttgg cgggcgcgac cgtctcggtc tggatctcgc cgctcatgcc
    12961 gcccgcgggt tcggacttgg actccgcgag cgcggccgcc cattccgcgg ccatcgcgtc
    13021 gtcgtcgctc atctcttcag ctgccattgc catctcccag ccagctcagg ttcgagctgg
    13081 tcaacaagcg atcgatcttg atcgcgtact tgcccgcgga cgtgccgtag tggcaatcga
    13141 acaccgggac gccgtcgacc ttggcctgga tcatcggatc caggtccagc tcgacgaaat
    13201 cgccgggctt gaacgacagc agttgctcca ccgtcgccgg cgcggtgccc agctcggcga
    13261 cgaggtcgac ctcggccgac tggatctgcg ccttgagcag gttgacccag cggcgatcgg
    13321 gctccacgga gtcgccctgg atcgtcgagt acaggacgtc gcggatcggc tccagcgtcg
    13381 aataaggaat gcagaagtgc accgagcccg aggtctcgcc cacttccagc gtgaagctcg
    13441 aagccacgac gatctcgctg ggcgtggcga tgttggcgaa ctgcggctgc atctccgagc
    13501 gctggtactc gaggtccagc gggtagatgc cctgccaagc gttgcggtac tcggtgatga
    13561 tgcagtcgac caggcgctgg atcacgcgct gctcggtggg cgagaagtcc cggccttcga
    13621 tgcgcgtgtg gaacttgccg acgccgccga acagcgcgtc gatcaccgcg aacaccaggc
    13681 tcgggtcgca cacgatcagg ccggagccgc gcagcggccg caccgacacg atgttgaagt
    13741 tggtcggcac cacgatctcg cgcaggaagg ccgagtactt ctgcaccttg atgccgttga
    13801 tcgagacttc cgggctcttg cggatgaagt tgaacaggcc gatgcggatg ttgcgcgcga
    13861 accgctcgcc gatgatctcc atcgtcggca tccggccacg gacgatccgc tcctggttgg
    13921 ccaggtcgta gttgcggatg ctgccttcga cctgctcttc ctgctcgagc ttctggcttt
    13981 cgcccgtgat gccctgcagg agggcatcga cttcgtcttg cgaaaggatc tgctggttca
    14041 tggctgcgcc gtgttcagtc gagcttgccc gcggtcactg gatgatgaag ctggagaact
    14101 gcaccgagcg gatcggcgcg ctgtcgttct cggcagcctt tttcttcttc tttttcttgg
    14161 ccggcgcggc ggcggaggcg ccgtcggcca ccggggccgc gtcgtcgtcc tcgtccacgt
    14221 cctcgcccat ggccttgagc gcctcgcggc ggatctgcct ggccagcagc tccttgccgt
    14281 cgccgcccgc caggtcggcc gacgacttgt gcgccagcag cagcaggatg ttgttgcgga
    14341 tggcgggcag gtaggtcttg atgtcctcgc tggcgtgctc gtcgggcacc tccagcacga
    14401 tgccgatctg cgcgtagcgg tcggcgtcgt ggtcggccag gttcacgacg aaggcgtcca
    14461 tcggcacgaa gacgggcttc tcgccctttt tcttcttctc ctcgtgcttg gccggcgccg
    14521 tgtcggcggc atcgccgtcc tcggccgcgg cggcttcggc ggcggccttc tgcttcttca
    14581 tgtagaccag cgcacccacg ccgccgccgc ccagcacgag cgccgcgacg gcgaccatga
    14641 ggaccagctt cttcttgcca cccgacttgg cggggacggc atctgcggca gcggctgcag
    14701 cggacatgga tacggctcct gacgaattct tggaacgcgc gcacgagcgc gcatcgttgg
    14761 agacgattat tgggatcggc gccccgttcg aatggctcga aaagcggcag aaaaaccgtc
    14821 agcccctaca gtccggccgt cagacgccga gcccctctcg ggggtcagcc cctccccggg
    14881 gtcaggtctt gaatattgcg gggtcaggtc ttgaatcgtg cagtcgcatc gcccgacggg
    14941 gcaggcacgg caaagaaggt cgctcctggc ggccctgcgg tcttgagccg gtatcggctc
    15001 gcgccaccgc gtctctgcgc tgctcgattc aagacttgac cccgcaatat tcaagacctg
    15061 accccatctc ggggc
//
LOCUS       DATCRO010000240         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_149100, whole genome shotgun sequence.
ACCESSION   DATCRO010000240 DATCRO010000000
VERSION     DATCRO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149100"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VIN75_06500"
     CDS             <1..1363
                     /locus_tag="VIN75_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY8973849.1"
                     /translation="MKDMSVGDAVHRYAILTVGDGMVSQIPSLLVSIAAGVVITRVDA
                     SGGDPQLANQIARQVLAHPRALVVAGLAFASFLLVPGFPAWAFGLLSAGVVAAGLLAR
                     RAHRWQRTPAWISHGDAEAPHAVAAPLVVRLAPDLRAGLDRHALDQRLDSTKQAVEGD
                     LGPVFPRLRVEHAGQMPPFGYEILVEDVPVSRGVLRPGWRLLDPAAATVPPAGAEVAE
                     PFGPFARVAWVRSPPADTPAWACEEVVCLHVDLVVRRHAPRLIGLQEVQRLLRMVQAD
                     APELAAEVTRAVPAQRIAEVLRRLLQEGIPVRNLRAIFESLALRGPAEADVIALTELV
                     RIDLGRYITSRHAGAARRIEAILFESGLLDRVLGAIEHSARGNLLLLSPAVAQDVRDQ
                     VRAILAGTAGAVVAIVSSDARRYVKTLIEPIAPDLPVLSYQELDPDVALHPVGWITNP
                     QAA"
     gene            1395..1661
                     /locus_tag="VIN75_06505"
     CDS             1395..1661
                     /locus_tag="VIN75_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEY8973850.1"
                     /translation="MTPQNVLDLTREGLQLVLWVSLPVVLVAALTALLVALVQAVTQI
                     QDQSIGQSIRLIAVMLALVMLAGLLGRNVLLFGERAFDSLVRMA"
     gene            1658..3499
                     /gene="sctC"
                     /locus_tag="VIN75_06510"
     CDS             1658..3499
                     /gene="sctC"
                     /locus_tag="VIN75_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HEY8973851.1"
                     /translation="MKRWLTLLAAGAVAFAGAAARAADLPWAGRPYQIVANEKPLADL
                     LRELSASQGTTAVIDPKVAGSISGRFSASPLKTLNSLCATYGLSWYYDGAFLYIDPSS
                     DARSEVLSIGNQAGNAGWLVQSLTQLRIYDSRYPLLVSGRDGTARVTGPKRYVEMVRQ
                     VARLLDSRSALNDGAEIRLFPLKYAWAGDFKITRSGKEVAVPGIAATLRSLYGRAGGG
                     GSSTGGATAAMSRQYAAYQSGADRQLKLSTGDVVNAPMVRLPGLGAAEQGDDGDAAAS
                     SGPAAISADNRQLPQFQADTRLNAVLVRDIPGHMAQYEKLIAAMDVRPRLIEIEVTIM
                     DVSTDTLDSLGVDWRAHSSHVDFQTGNGGNSPLTLGGATSESGQTGGLCCTNSQGLPT
                     TPLGSIFTAAIGNTLRDYLLARVSALAQTGNADVVARPKVLTLDNNEAILENLSDFYV
                     SVAGFQDASLFKVTTGTSVRVTPMIVDERNGRGVMMTIDIQDGNIDPTQSVGQIPVVR
                     HRTVDTQALIDEGSSLLLAGYTSESRSSAASGVPVLSSLPIIGHLFKYDQKQRNKEER
                     LYLLTPRLVTAGPAGNATGLVPLPVQDGPAATAPAGAPSAVSTGRRS"
     gene            3496..4605
                     /locus_tag="VIN75_06515"
     CDS             3496..4605
                     /locus_tag="VIN75_06515"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8973852.1"
                     /translation="MKPAPEFATPGWCLRFLSGAMKGRTIELRPGANAVGSAGDCDVM
                     LPGGDVLARHLVFNVGELVVSVHKAAEADVLLNREAMQQPRRSLAAGDVVSVGRIDLQ
                     LERSYPAPERTDPMFAGPESVLAGDAAAAPPDAGRGTALRVGAALLLVACAGLLGVGL
                     WAGGEDRPPVAASPSDVARAIAPFPELEAVAAPGGQVVVRGFVESRPRRRQLEQALAP
                     MGRRVSIEAVAVDDLVEQARRYLGNPALGVNYAGKGQLVVSGASDDDALRQRVARLTE
                     DLHPQVLVSDRVSWRPAPPPDRDAALRAHWEAWQGALPSRLVGITEDEAGLRSIQLAD
                     GTRYYEGAPLKSGALIAHIGADGLVLEGGARAETR"
     gene            4728..4931
                     /locus_tag="VIN75_06520"
     CDS             4728..4931
                     /locus_tag="VIN75_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973853.1"
                     /translation="MSTVDTSITAMQQAAADSARLQAASISVQTQIQGATTSAQTINS
                     SSAAASETAKGVASDTKAAARAS"
     gene            5052..5438
                     /locus_tag="VIN75_06525"
     CDS             5052..5438
                     /locus_tag="VIN75_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973854.1"
                     /translation="MTDPVAVLAASAPQAAAATSSPPTQFAPADTRMFASLMQGEPAL
                     DPAATATPGDVAHSLAAEFGNTRSFDEIRSSMLKAYDPRDPIKSMFTVASDGMEAQML
                     FSRLHIATSLASSATSLFGSLLKNQG"
     gene            5443..6222
                     /gene="sctJ"
                     /locus_tag="VIN75_06530"
     CDS             5443..6222
                     /gene="sctJ"
                     /locus_tag="VIN75_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HEY8973855.1"
                     /translation="MQHPNSWPRLAAGAALLLALAGCKVELYSGLGESDANEIVAALA
                     DGRIAARKEHVDGADWQVQVDEDRVGAALELLRAQGLPEQHYASMGDMFQKQGLVSTP
                     AEERMRYIFAVSQELSHTLRSIDGVVEARVHVVIPQDDPLSDKVRPSSAAVFIKHRPD
                     VDLRLLAPAVKDLVAHSIEGLSPDRVALTLFPAPRAASLAAGPAPEDVPREVLGMSQR
                     AVVTLLVLPVMAAIAIMALPTLLSRRGLDWRTWLQREVLRR"
BASE COUNT          847 a         2353 c         2301 g          865 t
ORIGIN      
        1 gatgaaggac atgagcgtgg gcgacgcggt gcaccgctac gcgatcctga ccgtgggcga
       61 cggcatggtc tcgcagatcc cctcgctgct ggtgtcgatc gccgcgggcg tcgtcatcac
      121 gcgcgtggac gcctccggcg gcgacccgca actggccaac cagatcgcgc gccaggtgct
      181 ggcgcatccg cgtgcgctgg tggtcgccgg cctggcgttc gcgagcttcc tgctggtgcc
      241 cggcttcccg gcctgggcgt tcgggctgct gtcggccggc gtggtggctg caggcctgct
      301 cgcccggcgc gcccaccggt ggcagcgcac gcccgcgtgg atctcgcacg gcgacgccga
      361 ggcgccgcac gcggtggccg cgccgctggt ggtgcgcctc gcgcccgacc tgcgcgccgg
      421 cctggaccgc cacgcgctcg accagcgcct ggactcgacc aagcaggccg tcgagggcga
      481 cctcggcccg gtgttcccgc gcctgcgcgt cgagcacgcc ggccagatgc cgccgttcgg
      541 ctacgagatc ctcgtcgagg acgtgcccgt ctcgcgcggc gtgctgcggc cgggctggcg
      601 gctgctggac ccggccgcgg cgacggtgcc gccggccggc gcggaagtgg ccgagccctt
      661 cggcccgttc gcgcgcgtcg cctgggtgcg cagcccaccc gcggacaccc ctgcgtgggc
      721 gtgcgaggaa gtcgtctgcc tccacgtcga cctggtggtg cgccgccatg cgccgcgcct
      781 gatcggcctg caggaggtgc agcgcctgct gcgcatggtg caggccgacg cgcccgagct
      841 cgccgccgag gtcacgcgcg ccgtgcccgc gcagcgcatc gccgaggtgc tgcggcgcct
      901 gctgcaggaa ggcatcccgg tgcgcaacct gcgcgcgatc ttcgaaagcc tggccttgcg
      961 cggaccggcc gaggccgacg tcatcgcgct gaccgagctg gtgcgcatcg accttggccg
     1021 ctacatcacc agccgccatg cgggcgccgc gcgccgcatc gaggccatcc tgttcgagtc
     1081 gggactgctc gatcgcgtgc tgggggccat cgagcactcc gcgcgcggca acctgctgct
     1141 gctgagcccg gccgtggccc aggacgtgcg cgaccaggtg cgcgccatcc tcgccgggac
     1201 ggccggcgcc gtggtggcga tcgtgtcgtc ggatgcgcgc cgctacgtca agacgctgat
     1261 cgagcccatc gcgcccgacc tgcctgtcct gtcctaccag gagctcgacc ccgatgtcgc
     1321 gctgcacccc gtcggctgga tcacgaatcc gcaggccgcc tgaccgatcc ttccttgacc
     1381 ggaccccgct cgacatgaca ccccagaacg tcctcgacct cacccgggaa ggcctgcaac
     1441 tggtcctgtg ggtctcgctg cccgtggtgc tggtggctgc gctgaccgcc ctgctggtgg
     1501 cgctcgtgca ggcggtcacg cagatccagg accagagcat cggccagtcc atccggctga
     1561 tcgcggtgat gctcgcgctc gtgatgctgg cgggcctgct gggccgcaac gtgctgctgt
     1621 tcggcgagcg cgcgttcgat tcgctggtgc gcatggcatg aagcgctggc tgacgcttct
     1681 cgcggccggc gcggtcgcgt tcgccggagc cgccgcgcgc gcggccgacc tgccgtgggc
     1741 cggcaggccg taccagatcg tcgccaacga aaagccgctg gccgacctgc tgcgcgagct
     1801 gtcggcctct cagggcacga cggcggtgat cgaccccaag gtcgccggct cgatcagcgg
     1861 gcgcttcagc gcctcgccgc tgaagacgct caacagcctg tgcgccacct acggcctcag
     1921 ctggtactac gacggcgcct tcctctacat cgatccgtcg tccgacgcgc gcagcgaggt
     1981 gctgtcgatc ggcaaccagg cgggcaacgc cggctggctg gtgcagtcgc tcacgcagct
     2041 gcgcatctac gactcgcgct acccgctgct ggtcagcggg cgggacggca cggcgcgcgt
     2101 gacgggcccc aagcgctacg tggagatggt gcgccaggtg gcgcggctgc tcgactccag
     2161 gagcgccctg aacgacggcg ccgagatccg cctgttcccg ctgaaatacg cgtgggcggg
     2221 cgacttcaag atcacgcgtt cgggcaagga ggtcgcggtg ccgggcattg ccgcgacgct
     2281 gcgcagcctg tacgggcgcg cgggcggcgg cgggtcgtcg accggcggcg cgaccgcggc
     2341 gatgtcgcgc cagtacgccg cctaccagtc cggcgccgat cgccagctca agctgtccac
     2401 gggcgacgtc gtcaatgcgc cgatggtgcg cctgcccggc ctgggcgcgg ccgagcaagg
     2461 cgacgacggc gacgccgcgg cgtcctccgg cccggccgcc atctcggcgg acaaccgcca
     2521 gctgccgcag ttccaggccg acacgcgcct gaacgcggtg ctggtgcgcg acatccccgg
     2581 ccacatggcg caatacgaga agctgatcgc cgcgatggac gtgcgcccgc gcctgatcga
     2641 gatcgaggtc acgatcatgg acgtcagcac cgacaccctg gacagcctcg gcgtcgactg
     2701 gcgcgcgcac tcctcgcatg tggacttcca gaccggcaac ggcggcaatt cgccgctgac
     2761 cctcggcggc gccacctccg agtccggcca gaccggcggc ctgtgctgca cgaactcgca
     2821 gggcctgccg accacgccgc tgggctcgat cttcacggcg gcgatcggca acaccctgcg
     2881 cgactacctg ctcgcccggg tcagcgcgct ggcgcagacc ggcaacgccg acgtcgtggc
     2941 ccggcccaag gtgctgacgc tggacaacaa cgaggcgatc ctcgagaacc tcagcgactt
     3001 ctacgtgagc gtcgccgggt tccaggacgc gagcctgttc aaggtcacca ccggcacctc
     3061 ggtgcgcgtg acgccgatga tcgtcgacga gcgcaacggg cgcggcgtga tgatgaccat
     3121 cgacatccag gacggcaaca tcgacccgac gcagtcggtg ggccagatcc cggtcgtgcg
     3181 ccaccgcacc gtggacaccc aggcgctgat cgacgaagga tcgagcctgc tgctggcggg
     3241 ctacacgtcg gagtcgcgca gcagcgcggc ctcgggcgtg ccggtgctct cgagcctgcc
     3301 gatcatcggc cacctgttca agtacgacca gaagcagcgc aacaaggagg agcgcctcta
     3361 cctgctgacg ccgcggctgg tgacggccgg gccggccggc aatgcgacgg ggctcgtgcc
     3421 gctgccggtg caggatggtc cggcagcgac ggcgccggcg ggtgcgccgt ccgccgtctc
     3481 gaccgggagg cgctcgtgaa gcccgcaccc gagttcgcca cgcccggctg gtgcctgcgc
     3541 tttctcagcg gggcgatgaa gggccgcacg atcgagctgc gacccggcgc gaacgccgtc
     3601 ggctccgcgg gcgactgcga cgtgatgctg cccggcggcg acgtgctggc gcgccacctc
     3661 gtcttcaacg ttggcgagct ggtggtgtcc gtgcacaagg ccgcggaggc ggacgtgctg
     3721 ctcaatcgcg aggcgatgca gcagccgcgc cgcagcctcg cggccggtga cgtggtgagc
     3781 gtcggccgca tcgacctgca gctcgagcgc agctaccccg cgcccgagcg caccgacccg
     3841 atgttcgcgg gccccgagag cgtgctcgcc ggcgatgcgg cggcggcgcc gccggatgcc
     3901 ggccgcggca cggcgctgcg cgtcggggcg gcgctgctgc tggtcgcctg cgcggggctg
     3961 ctgggcgtgg gcctgtgggc cggcggcgag gatcggccgc ccgtggccgc gagtccgtcc
     4021 gacgtggcgc gcgccatcgc gccgttcccc gagctggagg ctgtggccgc gccgggcggg
     4081 caggtcgtgg tcaggggatt cgtcgaatcg cgcccgcgtc gccggcagct ggagcaggcg
     4141 ctcgcgccga tggggcggcg cgtctccatc gaggccgtgg cggtcgacga cctcgtcgag
     4201 caggcgcggc gctacctcgg caacccggcg ctgggtgtga actacgcggg caagggccag
     4261 ctggtggtct cgggtgcgag cgacgacgac gcgctgcgcc agcgcgtggc gcggctgacg
     4321 gaagacctgc atccgcaggt gctggtgtcg gaccgcgtct cctggcgccc ggcgccgccg
     4381 cccgaccgcg acgccgcgct gcgcgcgcac tgggaggcct ggcaaggcgc gctgccctcg
     4441 cgcctggtgg gcatcaccga ggacgaggcc gggctgcgct cgatccagct ggccgacggc
     4501 acgcgctact acgagggcgc gccgctgaag tcgggcgcgc tgatcgcgca catcggcgcc
     4561 gacgggctcg tgctcgaggg aggcgcgcgc gccgagacca gatgaatgca ttcgcagcgg
     4621 gacgccccgg cggcccgcgc cacaggattc gcggcggtcg tccgcctcga atacccctgg
     4681 gagcatccgc accccggggc actttcaaac ccaacaggag agagctcatg tccaccgtcg
     4741 atacctccat caccgcgatg caacaagccg ccgccgacag cgcgcgcctg caggccgcca
     4801 gcatcagcgt gcagacgcag atccagggcg ccaccacctc ggcgcagacc atcaacagct
     4861 cgagcgccgc cgccagcgag accgccaagg gcgtcgccag cgacacgaag gcggctgcgc
     4921 gcgcgtcctg acgccgcgcg gcgtccctcg cgcgaccgcg tgtccgcgag ggcgccgcac
     4981 gggcgagccc gcgccccgcg ccggtctcgc ccgtgctgcc acatcccgat ccaaccaacg
     5041 aggggccagc catgaccgat cccgtcgccg tcctcgccgc gagcgcgccc caggccgccg
     5101 ccgcgacgtc ctccccgccg acccaattcg cgccggccga cacgcgcatg ttcgccagcc
     5161 tgatgcaggg cgagcccgcg ctcgatccgg ccgcgacggc cacgcccggc gacgtcgcgc
     5221 attccttggc cgccgagttc ggcaacacgc gctcgttcga cgagatccgc agcagcatgc
     5281 tcaaggccta cgacccgcgc gatccgatca agagcatgtt caccgtcgcc agcgacggca
     5341 tggaggcgca gatgctgttc tcgcgcctgc acatcgccac cagcctggcg agctcggcca
     5401 ccagcctgtt cggcagcctg ctgaagaacc agggctgacg ccatgcaaca cccgaattcc
     5461 tggcctcgcc tggccgccgg cgccgcgctg ctgctcgcgc tggccggctg caaggtcgag
     5521 ctgtattccg gcctcggcga gagcgacgcc aacgagatcg tcgccgcgct ggccgacggg
     5581 cgcatcgccg cgcgcaagga gcacgtcgac ggcgccgact ggcaggtgca ggtcgacgag
     5641 gaccgcgtcg gcgccgcgct ggaactgctg cgcgcgcagg gcctgccgga gcagcactac
     5701 gcgagcatgg gcgacatgtt ccagaagcag ggcctggtct ccacgcccgc cgaggaacgc
     5761 atgcgctaca tcttcgcggt gtcgcaggag ctgtcgcaca ccttgcgctc catcgacggc
     5821 gtggtcgagg cgcgggtgca cgtcgtgatc ccgcaggacg acccgctcag cgacaaggtg
     5881 cggccgtcat cggcggccgt gttcatcaag caccggcccg acgtcgacct gcggctgctg
     5941 gcgccggcgg tcaaggatct ggtcgcgcac agcatcgagg ggctttcgcc ggaccgggtg
     6001 gcgctgacgc tgttcccggc gccgcgcgcc gcctcgctcg cggccggccc cgcgcccgag
     6061 gacgtgccgc gcgaggtgct gggcatgtcg cagcgcgcgg tggtgacgct gctcgtgctg
     6121 cccgtgatgg ccgcgatcgc catcatggcg ctgccgacgc tgctgtcgcg ccgtgggctg
     6181 gattggcgca cgtggctgca gcgagaggtg ctgcggcgat gacggacctc gtcgggccgc
     6241 ccgacggcgg gccgctgggc ctgatgcggc tggtgctgcg cttgaacctg catcccgatg
     6301 ccggcatgca tccgagctgg ctgcccgcgg actggccggt gtcggcgcgc gcgcggctgg
     6361 gccggc
//
LOCUS       DATCRO010000241         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1026377, whole genome shotgun sequence.
ACCESSION   DATCRO010000241 DATCRO010000000
VERSION     DATCRO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026377"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VIN75_06535"
     CDS             <1..882
                     /locus_tag="VIN75_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8973856.1"
                     /translation="GGAFGAYMFGVGSTEMLGVVVTGEIWLRVPETIQMWWDGALPAG
                     VTAKDMMLHMIGRFGMNGGRYQAVEFAGPAVAALSMQERMTLSNMSAELGAQAGLIAP
                     DATTAAFLAEAGAPSADTGAWFTDDGAELTRHGFDAGALEPHVAAPHSPANSRGVSHY
                     AGTPVDVAYIGACTGAKLDDLRAAARVLRGHRVAAGVRLIVAPASIRDQERARAEGVL
                     QALTDAGAELFPTACGACSGYGDPMGDDITVISTTARNFKGRMGSPSAQVYLGSPYTV
                     AAAALRGRVTDPREVLA"
     gene            938..1459
                     /locus_tag="VIN75_06540"
     CDS             938..1459
                     /locus_tag="VIN75_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEY8973857.1"
                     /translation="MSVTHATHRVWRLGADIDTDLLAPGHAMKHGIDIIARHCLEAVR
                     PDFAAGVQRGDVIVAGPNFGIGSSREQAAAVLVHLGVRAVIAPSYSGLYFRNAFNVGL
                     LLLTCAQAETLGEGDRIALDATVPGVVGPDGRPLACEPVPGFLMDMVRAGGLLNQLRL
                     RAGRPASKKAPDA"
     gene            1452..>2566
                     /locus_tag="VIN75_06545"
     CDS             1452..>2566
                     /locus_tag="VIN75_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8973858.1"
                     /translation="MPDTSSTTTIDPVTLAVLRGRLEQVADEMDATLYRSAFNPIIAE
                     AHDACHGLYDAATGDTLVQGKSGLPVFVGAMAFAVRATVKAAEPRGGMRDGDIWISND
                     AYDGGTHANDFKLVKPFFRNGRLYCHMASAAHWHDVGGAVPGNYNPAATECWQEAVQI
                     PPVRIVRDGVLDADVLAILQANTRLPDSLWGDLNGQLAALELGAKRLDGLLDEYGDEL
                     VLSALGELRGRALRLMRSHIASLPDGRYSVEDVLDNDGIVNEPLTIALDMTVAGDRLT
                     LDFSRTSAQCAGPVNISRATAVAACYVALKHLFPDVPANAGVLDAVDFVIPGRLVISA
                     ERPRPVGGYTETILRMIDVIFSAAAQADPTRAVAQAYG"
BASE COUNT          368 a          939 c          908 g          351 t
ORIGIN      
        1 ggcggtgcct tcggcgccta catgttcggc gtgggcagca ccgagatgct cggcgtggtg
       61 gtcaccggcg agatctggct gcgcgtgccc gagaccatcc agatgtggtg ggacggcgcg
      121 ctgcccgcag gcgtcaccgc caaggacatg atgctgcaca tgatcggccg cttcggcatg
      181 aacggcgggc gctaccaggc ggtggagttc gccgggcccg cggtggcagc gctgtcgatg
      241 caggagcgca tgacgctgtc gaacatgagc gcggagcttg gcgcgcaggc cggcctgatc
      301 gcgccggacg ccaccacggc agccttcctc gccgaggccg gcgcaccgtc ggccgacacg
      361 ggggcttggt tcaccgacga cggtgccgaa ctcacgcgcc acggcttcga cgcgggcgcg
      421 ctcgagccgc acgtggccgc gccgcacagc cccgccaact cgcgcggggt gagccactac
      481 gccggcacgc cggtggacgt ggcctacatc ggcgcatgca ccggcgccaa gctcgacgac
      541 ctgcgcgccg cggcacgggt gctgcgcggg cacagggtgg ctgcgggcgt ccggctgatc
      601 gtcgcgcccg ccagcatccg cgaccaggag cgcgcgcgcg ccgagggcgt gctgcaggcg
      661 ctgacggacg cgggcgcgga gctcttcccg accgcgtgcg gggcctgctc gggctacggc
      721 gacccgatgg gcgacgacat caccgtcatc tcgaccacgg cgcgcaactt caagggccgc
      781 atgggttcgc ccagcgcgca ggtctacctg ggttccccct acaccgtggc ggccgcggcg
      841 ctgcgcggcc gcgtgaccga cccgcgcgag gtgctggcat gagccgccgg gccgttcctg
      901 ggtcgagtac cgcggtgcga cgcgcggagg tcgcagcatg agcgtcacgc acgccacgca
      961 ccgcgtctgg cggctcggtg ccgacatcga caccgacctg ctcgcgccag gccatgccat
     1021 gaagcacggc atcgacatca tcgccaggca ttgcctcgag gccgtccgcc ccgatttcgc
     1081 cgccggcgtg cagcgcggcg acgtgatcgt ggccggcccc aacttcggca tcggctcgtc
     1141 gcgggagcag gccgccgcag tgctcgtcca cctgggcgtg agggcggtga tcgcgccgtc
     1201 gtacagcggc ctgtatttcc gcaacgcctt caacgtgggc ctgctgctgc tgacctgcgc
     1261 gcaggccgag accctgggcg agggcgaccg gatcgccctc gacgccacgg tgcccggggt
     1321 cgtcggaccg gacggcaggc ccctggcctg cgagccggtg cccggattcc tcatggacat
     1381 ggtccgcgcc ggcggactgc tgaaccagct gcgcctgcgc gctggccgcc ccgcctcgaa
     1441 gaaagctccc gatgcctgat acctcctcca cgacaaccat cgatcccgtc acgctcgccg
     1501 tgctgcgcgg ccgcctcgag caggtggccg acgagatgga cgccacgctc taccgcagtg
     1561 cgttcaatcc catcattgcc gaggcgcacg acgcctgcca cggcctgtac gacgccgcca
     1621 ccggcgacac gctggtgcag ggcaagtcgg gcctgcccgt cttcgtggga gccatggcct
     1681 tcgccgtgcg cgccacggtg aaggcggccg agccgcgcgg cggcatgagg gacggcgaca
     1741 tctggatcag caacgacgcc tacgacggcg gcacgcacgc caacgacttc aagctggtga
     1801 agcccttctt ccgcaacggc aggctctact gccacatggc ctccgccgca cactggcatg
     1861 acgtgggcgg cgcggtgccg ggcaactaca acccggcggc caccgagtgc tggcaggagg
     1921 cggtgcagat cccgccggtg cgcatcgtgc gcgacggcgt gctcgacgcc gacgtgctgg
     1981 ccatcctgca ggccaacacg cgcctgcccg acagcctgtg gggcgacctg aacggccagc
     2041 tcgcggcact ggagctgggc gcgaagcgcc tggatggcct gctcgacgaa tacggcgacg
     2101 agctggtgct gagtgcgctg ggcgaattgc gcggccgcgc actgcgcctg atgcgctcgc
     2161 acatcgcctc gttgcccgac ggccgctaca gcgtcgagga cgtgctggac aacgacggca
     2221 tcgtcaacga gccgctgacc atcgcgctcg acatgacggt ggctggcgac cggctcacgc
     2281 tggacttctc ccgcacctcg gcgcagtgcg cgggcccggt gaacatctcg cgcgccaccg
     2341 cggtggccgc ctgctacgtg gcgctcaagc atctgttccc cgacgtgccg gccaatgccg
     2401 gcgtgctcga cgcagtggac ttcgtcattc ccgggaggct ggtgatcagc gccgagcgac
     2461 cgcgcccggt gggcggctac accgagacca tcctgcgcat gatcgacgtg atcttcagcg
     2521 ccgccgcgca ggccgacccc acgcgcgccg tggcgcaggc ctacgg
//
LOCUS       DATCRO010000242         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_227551, whole genome shotgun sequence.
ACCESSION   DATCRO010000242 DATCRO010000000
VERSION     DATCRO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227551"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VIN75_06550"
     CDS             complement(<1..284)
                     /locus_tag="VIN75_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY8973859.1"
                     /translation="MSKPSLAYTRGAQLPELLKQRIVIIDGAMGTMIQRYKLQEADYR
                     GERFQDHPKDIKGNNEMLQLVKPEVLREIHSQYLAAGADIIETNTFGATSV"
     gene            complement(643..933)
                     /locus_tag="VIN75_06555"
     CDS             complement(643..933)
                     /locus_tag="VIN75_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HEY8973860.1"
                     /translation="MTTMVYNSENFVVVELDVPVAPREGDTLEASDAPLSRGGYEIVD
                     KFARKEIYIEGAMAQHFREGVEELARGEPTQEQVEEFIGRYAGLPQQPLVLH"
     gene            complement(983..1999)
                     /locus_tag="VIN75_06560"
     CDS             complement(983..1999)
                     /locus_tag="VIN75_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973861.1"
                     /translation="MRTRCLLGLALVVLALHALAWRIAVGALPPLALPAAGVHRLALM
                     SLPQRAAAPMRVVQAPVSKPVQMAERPTAADRAFTPAPVRPRVTPGARAPAGAGGIRR
                     RAPSSAHAWPVYATRPPPSATLRYALTQSGVASASAGRAELEWRRGVAGYTLRLATAV
                     DGRPPREWQSSGTFDAAGVAPLRLVERERGRDRRGLAFDRDAGRVHFFGAPGAPVIAA
                     GAQDRWSWVAQLAAIAEAQAARGPPPDHWDLQVAGLRGDLERWTFRVLPAGDPPPELR
                     GNDLSAPGRRAPALLHVLRANEQPYDLRIEAWLAPSLHHLPAGLRMSTPPGPWSLSLW
                     QRSS"
     gene            2056..>2707
                     /locus_tag="VIN75_06565"
     CDS             2056..>2707
                     /locus_tag="VIN75_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8973862.1"
                     /translation="MKLATYKDGSRDGQLVVVSRDLSQAHFATGIATRMQQALDDWNF
                     IAPLLQDLSVALNHGKTRHAFPFVPEQCMAPLPRAYQWADGSAYLNHVELVRKARGAE
                     MPESFFGDPLMYQGGSDDFLGPCDPATFASADWGIDFEGEVAVVTGDVAMGASPEQAL
                     EGVRLVMLANDWSLRNLIPPELAKGFGFLQSKPATSFSPVAVTPDELGDAWKGGRVH"
BASE COUNT          391 a          951 c          979 g          386 t
ORIGIN      
        1 accgaggtgg cgccgaaggt gttggtctcg atgatgtcgg cgccggcggc caggtactgc
       61 gagtggatct cgcgcagcac ctcgggcttg accagctgca gcatctcgtt gttgcccttg
      121 atgtccttgg gatggtcctg gaagcgctcc ccgcggtagt cggcctcctg cagcttgtag
      181 cgctggatca tcgttcccat cgcgccgtcg atgatgacga tgcgctgctt gagcagctcg
      241 ggcagctgcg cgccgcgggt gtaggcgagg gagggcttgg acatgccgcg aattattgca
      301 ggacgtccaa gaccgtgtcg ggcccgtcgg gaaggaacag cgagtcgcat ccggcgctgg
      361 caggacggtt gcagcgcgtc gcgcacgcga acctgccggc cgccagcccg ggcggcgccg
      421 acgcggtggc gcttcgcggg ggcctttgcc tcaattgccc ggcagccgca ttgccgatgg
      481 cgaccaagcg acggcgacct gagggaaggc caggcgcctc tgcctcgggc gacgctctca
      541 tcgcgacccg ccttggaggg cgctgctgcc atcgccagaa agaccgaggc cgcgaacgac
      601 gccttccggc gcccttcgcg gcctcgatcg ggttcggccc gctcaatgca ggacgagcgg
      661 ctgctgcggc agtccggcgt agcggccgat gaattcctcg acctgttcct gcgtcggttc
      721 gccgcgcgcc agttcctcca cgccttcgcg gaaatgctgg gccatggcac cctcgatgta
      781 gatctccttg cgggcgaact tgtcgacgat ctcgtagccg ccgcgcgaga gtggtgcgtc
      841 gctggcctcg agcgtgtcgc cttcgcgcgg cgccaccggc acgtcgagtt cgacgaccac
      901 gaagttctcg gagttgtaga ccatggttgt catgagggat ccttctggtt cgagatggaa
      961 cttggtggcg tcgagctgca attcaagagc tgcgctgcca gagcgagagc gaccagggcc
     1021 ccggcggggt gctcatgcgc agtccggcgg gcaggtggtg cagcgaaggg gccagccagg
     1081 cttcgattcg caggtcgtag ggttgttcgt tggcacgcag gacatgaagc aaggcggggg
     1141 cccggcgccc gggtgctgac agatcgttac ctcggagctc gggtggcgga tcgccggccg
     1201 gcagcacgcg gaacgtccag cgctcgaggt cgccgcgcag gccggccacc tgcaggtccc
     1261 agtggtcggg cggcggcccg cgcgcggctt gcgcctcggc gatggcggcg agctgggcga
     1321 cccagctcca gcgatcctgt gccccggcgg cgatcacggg cgcacccggc gcgccaaaga
     1381 agtgcacccg gcccgcgtcg cggtcgaagg cgaggccgcg ccggtcgcgt ccccgctcgc
     1441 gttcgaccag gcgcagcggg gccacgcccg cggcgtcgaa cgtgccgctg gactgccatt
     1501 cgcgcggcgg ccggccgtcg acggcggtgg ccaggcgcag cgtatagccg gcgacaccac
     1561 ggcgccattc gagctcggcg cggccggcgc tcgccgatgc cacgcccgac tgcgtcagcg
     1621 cgtagcgcaa cgtggccgac ggcggcgggc gcgtggcgta gacgggccag gcgtgcgcgc
     1681 tcgacggcgc gcgccgccgg atgccgcctg cgcccgcggg agcgcgcgcc ccgggcgtga
     1741 cgcgcgggcg gaccggcgcg ggcgtgaacg cgcgatcggc cgcggtgggc cgctccgcca
     1801 tctgaaccgg tttcgacaca ggcgcctgca cgacccgcat gggggccgcc gcgcgctgcg
     1861 gcaaactcat gagcgccagc cggtgcacgc cggccgccgg cagcgccaac gggggcagcg
     1921 cgccgacggc gatccgccag gccagcgcgt gcaacgccag caccaccagc gccaatccga
     1981 ggagacatcg cgtccgcatc ggacaatact gccatcgccc ttccagaacc cccggcgtca
     2041 aaaccgggca tttccatgaa acttgccacc tacaaggacg gatcgcgcga cggccagctg
     2101 gtcgtcgtct cgcgcgacct cagccaggcc catttcgcca ccggcatcgc cacgcggatg
     2161 cagcaggcgc tcgacgactg gaacttcatc gcgccgctgc tgcaggacct gtcggtggcg
     2221 ctgaaccacg gcaagacgcg ccacgcgttc ccgttcgtgc ccgagcaatg catggcgccg
     2281 ttgccccgcg cctaccagtg ggcggacggc tcggcctacc tgaaccatgt cgagctggtg
     2341 cgcaaggccc gcggcgccga gatgcccgag agcttcttcg gcgacccgct gatgtaccag
     2401 ggcggcagcg acgacttcct cggcccgtgc gaccccgcca ccttcgcgag cgccgactgg
     2461 ggcatcgact tcgagggcga ggtcgccgtg gtgaccggcg acgtcgccat gggcgcgagc
     2521 cccgagcagg cgctcgaggg cgtgcggctg gtgatgctgg ccaacgactg gagcctgcgc
     2581 aacctcatcc cgcccgagct cgccaagggc ttcggcttcc tccagagcaa gccggcgacg
     2641 tcgttcagcc cggtggccgt gacacccgac gagctcggcg acgcctggaa gggcggccgc
     2701 gtgcacc
//
LOCUS       DATCRO010000243         6794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_452643, whole genome shotgun sequence.
ACCESSION   DATCRO010000243 DATCRO010000000
VERSION     DATCRO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452643"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1002)
                     /gene="cobA"
                     /locus_tag="VIN75_06570"
     CDS             complement(88..1002)
                     /gene="cobA"
                     /locus_tag="VIN75_06570"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY8973863.1"
                     /translation="MNDAMTTAAHPVHLVGAGPGDPELLTLKALRVLRQASVILVDDL
                     VGEQVLAAALDGLGAPPRVVRVGKRGGCASTPQEFIEKLMVREALAGERVVRLKGGDP
                     LVFGRAGEEIAALRAAGIDVEIVNGITSGLAAAGSAGIGWTDRRAGAQGVLLVTGHAG
                     QGCEEPDWDAIARVAAAGVTLVVYMGVARAEAIVERLRTALPGELPVAIVQRATGADE
                     VIARTTLGGLVDTIRTEAIASPAILVIGRVAAEARVPVRLPEDVHSAHPTNWGRGPAV
                     LTLRGLGRWDRGLGAEENHVAGADSLRR"
     gene            complement(999..3845)
                     /locus_tag="VIN75_06575"
     CDS             complement(999..3845)
                     /locus_tag="VIN75_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8973864.1"
                     /translation="MGAPRIIPIAGGPRETRSTCPYCGTGCGVIIRSEGAAIVDVRGD
                     PDHPANFGRLCSKGSSLHLTAAPHVSAQARLLQPMRRDRRGAAPRAIGWDDANQLLAD
                     RIVSTVDKHGADAIGLYISGQLLTEDYYVFNKLAKGLLGTNNIDTNSRLCMSSAVSGY
                     KATLGADAPPACYEDLAHADLVYIAGSNLAWAHPILCRRLEDAKAARPSQKWIVVDPR
                     KTETAAMADLHLQILPGSDIALSHGLLHLMLWEGYADQAFIRAHTTGFEALRDRVREC
                     TVRETARVTGLREEDIVTAARWWGESAAVLSMYCMGLNQSAQGTANNGALINLHLATG
                     QVGRPGAGPFSLTGQPNAMGGREVGGLANLMSAHRDMANAGHRAEIAALWGVDDVPSR
                     PGRTAVEMFQAAADGQIKLLWIACTNPAQSMPDQATVRRAFERAEFVVLQEAFATTAT
                     ADFADLLLPASTWGEKEGSVTNSERRISRVRPAVPAPGQARADWRIVVDVARRVERRL
                     RPASRSLFPYDDAEAIWNEHRETTRGRDLDITGLSYALIERDGPQQWPFPEGASTGAA
                     RLYTDGRFATPDGRARFFDAEARLPAEPRDARFPFSLTTGRLRDQWHGMSRTGTLGRL
                     FGHVGAPSIDLCAQDMARLNLGDGDLVRVTSRRGELVLPARRSASQISAQAFIAMHWG
                     SEVLTGRGDEADPFAGINSVTSPACCPTSRQPELKHCAVRIARAGLPWRLRAMAWLPQ
                     DEALARRERLRPQLAQFGYAACLPFGREPDAQGRVGLWLDIADKQAPDDIALNALRVE
                     LGLEASAVLDYRDPRTGVQRALRVGRDAAGAQWLQGFWIAGGQAADAALAAWWRETLQ
                     GDAPLAMPAHRLLAPGAREGRADAAPASPQLCTCFDVSVAQVTAALAVANGDAAARLA
                     QAQDQLRCGTHCGSCLPRLRGLAQQACELSPATP"
     gene            complement(3850..4248)
                     /gene="nirD"
                     /locus_tag="VIN75_06580"
     CDS             complement(3850..4248)
                     /gene="nirD"
                     /locus_tag="VIN75_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEY8973865.1"
                     /translation="MNDWIEICRVDDIPVLGARRVRRPAGTDVAIFRTDSDRVFALLD
                     RCPHKGGPLSQGIVFGEHVACPLHNWAISLEDGQARAPDVGCATRFSVRVEGGAVSLL
                     ASELATLAIDDADAAAALAAARKVIAIASA"
     gene            complement(4263..6701)
                     /gene="nirB"
                     /locus_tag="VIN75_06585"
     CDS             complement(4263..6701)
                     /gene="nirB"
                     /locus_tag="VIN75_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEY8973866.1"
                     /translation="MKKLKLVMIGNGMAGVRTLEELLKIAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTIAEIVLNPLAWYGDNGITLHLGKAVTRIDRVRRVVHAADGTQAPY
                     DRLLIATGSTPFMLPVPGKDLEGVIGYRDIADTEAMIDAATRYKHAVVIGGGLLGLEA
                     ANGLMLRGMKVTVVHIAPWLMERQLDDVAGGLLRQSLVDRGLDFLIGAQTEALLADDA
                     TGRVRAVRFKDGNEVPADLVVMAAGIRPNTALAESTGLHCQRGIVVTDTLQTVTDARI
                     WAVGECAAHRGTAYGLVAPLFEQAKVCATHLAEFGIGRYLGSQVSTKLKVTGIDLFSA
                     GDFMGGEGCDSIVLSDPQGGVYKKLVIKDDKLVGACLYGDTVDGSWYFKLLREGRSVS
                     DIRDRLMFGESSLGDAGHQGQNKAAAMADADEVCGCNGVTKGTICKAIKDKGLFTLDD
                     VRKHTKASASCGSCTGLVEQIIMATAGGDYSTAPRKKAMCGCTEHSHQDVREAIRKLH
                     LTTIADTQRALSWRTPNGCSSCRPALNYYLISTWPKEARDDPQSRYINERSHANIQKD
                     GTYSVIPRMWGGETTAAELRRIADVVDKYKIPTVKVTGGQRIDLLGVKKEDLVDVWKD
                     IGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTRLGKDLERGLWRMYAPHKVKLAVSG
                     CPRNCAESGIKDVGVIGVESGWELYVGGNGGIKTEAGQFFCKVRTAEEVMEVSGAFLQ
                     LYREEGWYLERTVHWIARVGLDSIKQRVLDDVANRKALWERLQFALDGEPDPWFEHEK
                     ASVDTRQFIPLVPV"
BASE COUNT          883 a         2452 c         2450 g         1009 t
ORIGIN      
        1 cacgcattct aggcaggccc cccaggatga gcatgggtgg aaaacaacaa ggccgggaca
       61 tgcctcacgg ccggcggcga tttttgttca ccgtcgtaac gagtcggccc cggcgacgtg
      121 attttcttcc gctcccaggc cccggtccca ccgcccaaga cctcgaagtg taagaaccgc
      181 gggccctctc ccccagttcg tgggatgggc cgaatgcaca tcttcgggca accgcaccgg
      241 cacgcgggcc tccgcggcca cccgcccgat cacgaggatc gcggggctgg cgatcgcctc
      301 ggtccggatc gtgtccacca gcccgcccag ggtcgtgcga gcgatcactt cgtccgcgcc
      361 ggtcgcgcgc tggacgatcg ccacgggcag ctcgccgggc agcgccgtgc gcaggcgctc
      421 gacgatggcc tccgcccgcg cgacgcccat gtagacgacc agcgtgacgc ccgcggccgc
      481 cacgcgggcg atcgcgtccc agtccggctc ctcgcagccc tgccccgcgt ggccggtcac
      541 cagcagcacg ccctgcgcgc cggcgcggcg gtcggtccag ccgatgccgg ccgaccccgc
      601 ggcggccagg ccggacgtga tgccgttgac gatctcgacg tcgatgccgg ccgcccgcag
      661 cgcggcgatc tcctcgccgg cgcggccgaa cacgagcgga tcgccgccct tgagccgcac
      721 gacgcgttcg cctgccaggg cctcgcgcac catcagcttc tcgatgaact cctgcggcgt
      781 cgatgcgcag ccgccacgct tgcccacgcg cacgacgcgc ggcggcgctc ccaggccgtc
      841 gagggcggcc gccagcacct gctcgccgac caggtcgtcg accaggatca ccgaggcctg
      901 gcgcagcacg cgcagcgcct tgagcgtgag gagctcggga tcgccggggc cggcgccgac
      961 gaggtggacg ggatgcgctg cggtggtcat cgcgtcgttc acggcgtggc gggggacagc
     1021 tcgcaggcct gctgcgcgag gccgcgcagc ctgggcaggc aggagccgca atgggtgccg
     1081 cagcgcagct ggtcctgcgc ctgggcgagt cgcgccgccg cgtcgccgtt ggcgacggcc
     1141 agcgcggcgg tgacctgcgc gacgctcacg tcgaagcagg tgcacagctg cgggctggcc
     1201 ggcgccgcgt cggcgcggcc ttcgcgcgcg ccgggcgcga gcagccggtg cgccggcatt
     1261 gcgagcggcg cgtcgccctg cagcgtctcg cgccaccacg cggcgagcgc cgcgtcggcc
     1321 gcttggccgc cggcgatcca gaagccttgc agccactgcg cgccggccgc gtcgcgaccg
     1381 acacgcaacg cgcgctgcac gccggtgcgc gggtcgcggt agtcgagcac ggccgaggcc
     1441 tcgagcccca gctcgacgcg cagcgcgttc agcgcgatgt cgtcgggcgc ctgcttgtcg
     1501 gcgatgtcca gccacaggcc gacgcggccc tgcgcgtcgg gctcgcggcc gaacggcagg
     1561 caggcggcgt agccgaactg cgcgagctgc ggacgcaggc gttcgcgccg cgccagcgcc
     1621 tcgtcctgcg gcagccaggc catcgcgcgc agccgccagg gcaggcccgc gcgcgcgatg
     1681 cgcacggcgc agtgcttgag ctcgggctgg cgtgaggtgg ggcagcaggc cggcgacgtc
     1741 accgagttga tgccggcgaa cgggtcggcc tcgtcgccgc ggccggtcag cacctcgctg
     1801 ccccagtgca tcgcgatgaa ggcctgtgcc gagatctggc tcgcgctgcg gcgcgccggc
     1861 agcacgagtt cgccgcggcg cgaggtgacg cgcacgaggt cgccgtcgcc caggttcagg
     1921 cgcgccatgt cctgcgcgca caggtcgatc gacggcgcgc cgacgtggcc gaacagccgc
     1981 cccagcgtgc cggtgcggct catgccgtgc cactggtcgc gcaggcgtcc ggtggtcagc
     2041 gagaacggga aacgcgcatc gcgcggctcg gcgggcagcc tggcctccgc gtcgaagaag
     2101 cgggcgcggc cgtcgggcgt cgcgaagcgg ccgtcggtgt acaggcgcgc ggcgccggtc
     2161 gacgcgccct ccgggaacgg ccactgctgc gggccgtcgc gctcgatcag cgcgtagctc
     2221 aggccggtga tgtccaggtc acggccgcgc gtggtctcgc ggtgctcgtt ccagatggcc
     2281 tccgcgtcgt cgtacgggaa cagcgagcgg ctcgccggcc gcaggcgccg ttcgacgcgg
     2341 cgcgcgacgt cgacgacgat gcgccagtcc gcgcgcgcct ggcccggcgc cggcaccgcc
     2401 ggacgcacgc ggctgatgcg gcgctcgctg ttggtcacgc tgccttcctt ctcgccccag
     2461 gtcgacgcgg gcagcagcag gtcggcgaag tccgccgtcg cggtcgtggc gaatgcctcc
     2521 tgcagcacga cgaactcggc gcgctcgaac gcacggcgca cggtggcctg gtcgggcatc
     2581 gattgcgccg ggttcgtgca ggcgatccac agcagcttga tctgcccgtc cgccgcggcc
     2641 tggaacatct cgacggccgt ccggcccggg cggctcggga cgtcgtcgac gccccacagc
     2701 gcggcgatct ccgcgcggtg tccggcgttg gccatgtcgc ggtgcgcgga catcaggttc
     2761 gcgagcccgc ccacctcgcg gccgcccatc gcgttgggct ggcccgtgag cgagaacggc
     2821 ccggcgccgg gccggccgac ctggccggtc gccaggtgca ggttgatcag cgcgccgttg
     2881 ttggcggtgc cctgcgcgct ctggttcagg cccatgcagt acatcgacag caccgccgcc
     2941 gactcgcccc accagcgcgc ggcggtgacg atgtcctcct cgcgcaggcc cgtcacgcgc
     3001 gcggtctcgc gcaccgtgca ctcgcgcacc cggtcgcgca gcgcctcgaa gccggtggtg
     3061 tgggcgcgga tgaacgcctg gtcggcgtag ccttcccaca gcatcaggtg cagcaggcca
     3121 tgcgacagcg cgatgtcgct gcccggcaga atctgcaggt gcaggtcggc catcgcggcc
     3181 gtctcggtct tgcgcgggtc gacgacgatc cacttctggg acggccgcgc cgccttggcg
     3241 tcctccagcc ggcggcacag gatcgggtgc gcccacgcga ggttgctgcc ggcgatgtag
     3301 acgaggtcgg catgcgcgag atcctcgtag cacgccggcg gcgcgtccgc gccgagcgtc
     3361 gccttgtagc ccgacaccgc gctggacatg cacagccgcg agttggtgtc gatgttgttg
     3421 gtgcccagca ggcccttggc gagcttgttg aagacgtagt agtcctcggt cagcagctgc
     3481 cccgagatgt acaggccgat cgcgtcggcg ccgtgcttgt ccaccgtgct gacgatgcga
     3541 tcggcgagca gctggttcgc gtcgtcccag ccgatcgcgc gcggcgcggc gccgcggcgg
     3601 tcgcgccgca tcggctgcag cagccgcgcc tgcgcgctga cgtgcggcgc ggccgtcagg
     3661 tgcagcgagc tgcccttgct gcacaggcgg ccgaagttgg ccgggtggtc ggggtcgccg
     3721 cgcacgtcga cgatcgcggc gccctcgctg cggatgatca cgccgcagcc ggtgccgcag
     3781 tatgggcagg tcgaccgcgt ctcgcgcggg ccgccggcga tggggatgat ccgcggcgcg
     3841 cccatgtgct caggcgctgg cgatggcgat caccttgcgc gcagcggcga gcgcggccgc
     3901 ggcgtcggca tcgtcgatgg ccagggtggc gagctcgctg gccagcaggc tcacggcgcc
     3961 gccctcgacg cgcaccgaaa agcgcgtcgc gcagccgacg tcgggcgccc gcgcctggcc
     4021 gtcctccagc gagatcgccc agttgtgcag cggacacgcc acgtgctcgc cgaacacgat
     4081 gccctggctc agcgggccgc ccttgtgcgg gcagcgatcg agcagcgcga acacgcggtc
     4141 gctgtcggtg cggaagatcg cgacgtccgt gcccgcgggg cgccgcacgc ggcgggcgcc
     4201 gagcacgggg atgtcgtcga cgcggcagat ttcgatccag tcgttcatgt tttcctgtct
     4261 cctcacacgg gcaccagcgg gatgaactgc cgcgtgtcca ccgatgcctt ctcatgttcg
     4321 aaccacgggt cgggctcgcc gtccagcgcg aactgcaggc gctcccacag cgccttgcgg
     4381 ttggcgacgt cgtcgagcac gcgctgcttg atggagtcga ggccgacgcg cgcgatccag
     4441 tgcaccgtgc gctccaggta ccagccttcc tcgcggtaca gctgcaggaa cgcgccgctg
     4501 acctccatca cttcctcggc cgtgcgcacc ttgcagaaga actgcccggc ctcggtcttg
     4561 atgccgccgt tgccgccgac atacagctcc cagccggact ccacgccgat cacccccacg
     4621 tccttgatgc cggattcggc gcaattgcgc gggcagcccg agacggccag cttgaccttg
     4681 tgcggcgcgt acatgcgcca caggccgcgc tcgaggtcct tgcccaaccg cgtcgagtcc
     4741 tgcgtgccga agcggcacca ctcgctgccc acgcaggtct tgaccgtgcg cagcgccttc
     4801 gcgtaggcgt ggccggaggg catgccgatg tccttccaga cgtcgaccag gtcctccttc
     4861 ttcacgccca gcaggtcgat gcgctggccg ccggtgacct tgaccgtcgg gatcttgtac
     4921 ttgtcgacca cgtcggcgat gcgccgcagc tcggctgccg tcgtctcgcc gccccacatg
     4981 cgcgggatca ccgagtacgt gccgtccttc tggatgttgg catgcgagcg ctcgttgatg
     5041 tagcggctct gcggatcgtc cctggcctcc ttgggccagg tgctgatcag gtagtagttc
     5101 agcgccggcc ggcacgacga gcagccgttg ggcgtgcgcc aggacagcgc gcgctgcgtg
     5161 tcggcgatgg tcgtcaggtg cagcttgcgg atcgcctcgc gcacgtcctg gtgcgagtgt
     5221 tccgtgcagc cgcacatcgc cttcttgcgc ggcgcggtgg agtagtcgcc gccggccgtg
     5281 gccatgatga tctgctcgac caggcccgtg cacgagccgc aggaggcgct ggccttcgtg
     5341 tgcttgcgca cgtcgtccag cgtgaacagg cccttgtcct tgatggcctt gcagatcgtg
     5401 cccttggtca cgccgttgca gccgcagacc tcgtcggcgt cggccatcgc ggcggccttg
     5461 ttctggccct ggtggccggc gtcgccgagg ctggattcgc cgaacatgag gcggtcgcgg
     5521 atgtcgctga ccgagcggcc ctcgcgcagc agcttgaagt accagctgcc gtcgacggtg
     5581 tcgccgtaca ggcaggcgcc cacgagcttg tcgtccttga tgacgagctt cttgtagacg
     5641 ccgccctgcg ggtcggacag cacgatggaa tcgcagccct cgccgcccat gaagtcgccg
     5701 gcggagaaca ggtcgatgcc ggtgaccttc agcttggtcg agacctggct gcccaggtag
     5761 cggccgatgc cgaactcggc caggtgcgtc gcgcagacct tggcctgctc gaacagcggc
     5821 gccacgaggc cgtaggccgt gccgcggtgc gcggcgcatt cgcccaccgc ccagatgcgc
     5881 gcgtcggtca cggtctgcag cgtgtcggtg acgacgatgc cgcgctggca gtgcaggccg
     5941 gtcgactcgg ccagcgccgt gttggggcgg atgccggcgg ccatcacgac gaggtcggca
     6001 ggcacctcgt tgccgtcctt gaagcgcacg gcgcggacgc gtccggtcgc gtcgtcggcc
     6061 agcagcgcct cggtctgggc gccgatcagg aagtccaggc cgcggtcgac cagcgactgg
     6121 cgcagcaggc cgccggccac gtcgtcgagc tggcgttcca tcagccacgg cgcgatgtgc
     6181 accaccgtga ccttcatgcc gcgcagcatc aggccgttgg ccgcttcgag gcccagcagg
     6241 ccgccgccga tgacgaccgc atgcttgtac cgggtggcgg catcgatcat ggcctcggtg
     6301 tcggcgatgt cgcggtagcc gatcacaccc tcgagatcct tgcccggcac cggcagcatg
     6361 aacggcgtgg agccggtggc gatcagcagg cggtcgtacg gcgcctgcgt gccgtcggcc
     6421 gcgtgcacga cgcggcgcac gcggtcgatg cgcgtgaccg ccttgcccag gtgcagcgtg
     6481 atgccgttgt cgccgtacca ggccagcggg ttcaggacga tctcggcgat ggtctgctcg
     6541 ccggccagca ccggcgacag caggatgcgg ttgtagttcg gatgcggctc ggcgccgaac
     6601 acggtgatgt cgtacaggtc gggcgcgatc ttcaggagct cctcgagcgt gcgcacgccc
     6661 gccatgccgt tgccgatcat caccagtttg agtttcttca tcgcggagcc tcttgaagcc
     6721 gaacgtggaa tcgcggaaag ccgatcaggc cgcctcggcc aggccgggcg tcgcgacggt
     6781 gcgcgcctcg ggcg
//
LOCUS       DATCRO010000244         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_576529, whole genome shotgun sequence.
ACCESSION   DATCRO010000244 DATCRO010000000
VERSION     DATCRO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576529"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIN75_06590"
     CDS             <1..322
                     /locus_tag="VIN75_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973867.1"
                     /translation="RPPGSLMAAAQTYDSVNLLVRALFATHGDTAPGAVRQALEQPRE
                     PHRGVVTTYDHPFGPRDHDAFSVNMIWLGVWHGGEIHYFHATDAGLSGAVRHKERPPG
                     SHRD"
     gene            445..618
                     /locus_tag="VIN75_06595"
     CDS             445..618
                     /locus_tag="VIN75_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEY8973868.1"
                     /translation="MRLLLLLPFIGLLAVPLFNTETPEFAGFPFFYWYMFAWVPLTSL
                     LIWLVNRNEKPEE"
     gene            623..2119
                     /locus_tag="VIN75_06600"
     CDS             623..2119
                     /locus_tag="VIN75_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY8973869.1"
                     /translation="MGDNINWTALSVFIFFFALVTVIGFLASRWQRGGAHKGAHLDEW
                     GLGGRNFGAWITWFLVGGDFYTAYTVIAVPALVYAVGAYGFFALPYTILVYPIVFTIM
                     PRLWAEAKRKGHVTAADVVYGRYGSRPLETAIALTGIVATMPYIALQLVGMEVVIKAL
                     GLTGELPLAAAFVILALYTYAAGLRAPALIAFVKDVMIYIVVLVAVVIVPMKVGGYGN
                     VFSSARDAFAAKGGATGLTLHPGQMLPFATLAIGSAMAAFMYPHTLTGIFAAKDASTI
                     RKNAIFLPAYTVLLGLIALLGYMAYAAHLAVKSNNDVVPALFNALFPSWFSGFAFSAV
                     AIGALVPAAVMSIGAANLFTRNFWKPYVNARVTPAQEEKVAKYASLVVKVGALLCILL
                     MPTKFALDLQLLGGVWILQTFPSLVFGLWSKRLQAPALLAGWLVGIVFGTWTAFADGV
                     KPVHSILVGGDKFTVYTGLLALVINVVVAVVVQTLVRSPAPAAASGKA"
     gene            2190..>2849
                     /locus_tag="VIN75_06605"
     CDS             2190..>2849
                     /locus_tag="VIN75_06605"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8973870.1"
                     /translation="MRLKAKLLVLAAVPLLLSLALIAAAVQLQQRELARREHALVEGG
                     YLSARRAELKNYVQLAMSAIAPMAESRDDVAGSQARAMRLLESMEYGDDGYFFVYDMH
                     GTVLMHPRQRDLVGQDLSGMRDPRGRATIQELLAGARAGGGFVEYMWRRPSTGRIAPK
                     LGYVTEIPQWDWMIGTGLYRDDIDSATAQLDAQASSNIGETLLWIAAIAFGGVALISF
                     SG"
BASE COUNT          392 a          983 c          987 g          487 t
ORIGIN      
        1 ccgtccgccg ggctcgctga tggccgcggc gcagacgtac gacagcgtga acctgctcgt
       61 gcgtgcgctg ttcgccacgc atggcgacac ggcacccggc gccgtcaggc aggcgctcga
      121 gcagccgcgc gagccgcatc gcggcgtcgt cacgacctac gaccacccgt tcgggccccg
      181 cgaccatgac gcgttcagtg tcaacatgat ctggctgggc gtctggcacg gcggcgagat
      241 ccactacttc cacgcgaccg atgccgggct ctcgggcgcg gtgcgccaca aggagcggcc
      301 gccgggttcg catcgtgact aggggttgtc ccctacgtgg tggcgcctgc gacgcaacgc
      361 gacatgacgg atgcggtgcg cgccccgaat ttcgacactg atggcagagc gggcagtgtt
      421 cccgccaaac cctggagaca agacatgcgc ctgctactgc tgctgccgtt catcggcctg
      481 ctggccgttc cgttgttcaa caccgagacg ccggagttcg ccggattccc gttcttctac
      541 tggtacatgt tcgcgtgggt gccgctcacc tcgctgctga tctggctggt caaccgcaac
      601 gagaagccgg aggagtgagg ccatgggcga caacatcaac tggaccgcgc tgtcggtctt
      661 catcttcttc ttcgcgctgg tgaccgtcat cggcttcctg gcctcgcgct ggcagcgcgg
      721 cggcgcgcac aagggcgcgc acctcgacga gtggggcctg ggcggccgca acttcggcgc
      781 ctggatcacg tggttcctgg tgggcggcga cttctacacc gcctacaccg tcatcgcggt
      841 gccggcgctg gtctacgcgg tcggcgccta cggcttcttc gcgctgccgt acaccatcct
      901 ggtctatccg atcgtcttca ccatcatgcc caggctgtgg gccgaggcca agcgcaaggg
      961 gcacgtgacg gccgccgacg tggtctacgg ccgttacggc tcgcgaccgc tggagacggc
     1021 gatcgcgctg accggcatcg tcgccaccat gccctacatc gcgctgcagc tggtgggcat
     1081 ggaggtggtg atcaaggcgc tgggcctgac cggcgagctg ccgctggccg ccgcgttcgt
     1141 catcctggcg ctgtacacct acgccgcggg cctgcgcgcg ccggcgctga tcgccttcgt
     1201 caaggacgtg atgatctaca tcgtcgtgct cgtcgcggtg gtcatcgtgc cgatgaaggt
     1261 gggcggctac ggcaacgtgt tctcctcggc gcgcgacgcg ttcgcggcca agggcggcgc
     1321 gacgggcctg acgctgcacc cgggccagat gctgccgttc gccacgctgg cgatcggctc
     1381 ggcgatggcg gcgttcatgt acccgcatac cctgacgggc atcttcgcgg ccaaggacgc
     1441 cagcacgatc cgcaagaacg cgatcttcct gccggcctac acggtgctgc tgggcctgat
     1501 cgcgctgctg ggctacatgg cctacgccgc gcacctggcg gtcaagagca acaacgacgt
     1561 cgtgcccgcg ctgttcaacg cgctgttccc gtcgtggttc tccggcttcg cgttctccgc
     1621 ggtggcgatc ggcgcgctcg tgccggcggc cgtgatgtcc atcggcgcgg ccaacctgtt
     1681 cacgcgcaac ttctggaagc cgtacgtgaa cgcgcgcgtc acgccggcgc aggaggagaa
     1741 ggtcgcgaaa tatgcgtcgc tggtggtcaa ggtgggcgcg ctgctgtgca tcctgctgat
     1801 gcccaccaag ttcgcgctgg acctgcaact gctgggcggc gtgtggatcc tgcagacctt
     1861 cccgtcgctc gtcttcggcc tgtggtcgaa gcgcctgcag gccccggcgc tgctggccgg
     1921 ctggctcgtg ggcatcgtgt tcggcacgtg gacggcgttc gccgacggcg tcaagccggt
     1981 gcactcgatc ctcgtcggtg gcgacaagtt caccgtctac accggcctgc tggcgctggt
     2041 catcaacgtg gtcgtggcgg tcgtcgtgca gacgctggtg cgctcgccgg cgccggcggc
     2101 ggcgtccggc aaggcctgag ccgcacctgg gccaggcatg ggccgcgcgg gcgtcgccga
     2161 cgcccgcgcg gccttgtcac aatcgcgcca tgcgtctgaa ggccaagctt ctcgtcctcg
     2221 cggcggttcc gctgctgctg tcgctcgcgc tgatcgccgc ggcggtgcaa ctgcagcagc
     2281 gcgagctggc caggcgcgag cacgccctgg tggaaggcgg ctacctcagc gcgcggcgcg
     2341 cggagctgaa gaactacgtg cagctcgcga tgagcgccat cgccccgatg gcggaatcgc
     2401 gcgacgacgt cgccggctcc caggcgcgcg cgatgcggct gctcgagtcc atggagtacg
     2461 gcgacgacgg ctacttcttc gtctacgaca tgcacggcac cgtgctcatg catccgcgcc
     2521 agcgcgacct ggtcggccag gacctgtcgg gcatgcgcga cccgcggggc cgcgcgacga
     2581 tccaggagct gctcgccggg gcgcgcgcgg gcggcggctt cgtcgagtac atgtggcggc
     2641 ggccgtcgac cggcaggatc gcgcccaagc tcggctacgt caccgagatc ccgcaatggg
     2701 actggatgat cggcaccggg ctgtaccgcg acgacatcga ctccgcgacg gcgcagctgg
     2761 acgcccaggc ctcgtccaac atcggcgaga cgctgctgtg gatcgcagcc atcgcgttcg
     2821 gcggcgtggc gctgatcagc ttcagcggc
//
LOCUS       DATCRO010000245         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_205361, whole genome shotgun sequence.
ACCESSION   DATCRO010000245 DATCRO010000000
VERSION     DATCRO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205361"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VIN75_06610"
     CDS             complement(<1..1291)
                     /locus_tag="VIN75_06610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8973871.1"
                     /translation="MPAPLSAVESSPELPTHADAVVIGGGIIGVFAAYYMAQRGVSVA
                     LIEKGRIGAEQSSRNWGWCRQQNRDARELPMATKSLDLWERFTQDTGEVTGFSRCGLL
                     YLSNSEKELDGWARWGEFARSVNVQTRMLSAGEAAQRGHATGKAWKGGVFAPTDGTAD
                     PSRAAPAVARAFMKLGGTVHQGCAARGLETEGGQVCAVVTESGTIRTRVAVLAGGAWA
                     SSFCRQYGIRFPQAAIRQTALSVTTSGKDIPAALHTTGVSMTRRADGGYTLAISGRGR
                     VDPTPQLLRFAPQFLPMFQRRWRNLAPGGLEGIRCGHEGFARWRLDETTPMERMRILD
                     PKADASVVDLTYRRAVELVPALADSSVTAAWAGYVDSTPDGVPGIGEMLSLRGLVLAA
                     GFSGHGFGIGPGAGHLIADIVSGASPLVDPRPYHPDRF"
     gene            complement(1369..>2758)
                     /locus_tag="VIN75_06615"
     CDS             complement(1369..>2758)
                     /locus_tag="VIN75_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8973872.1"
                     /translation="SAPDNEGPAGCGDGVDNVFGISKTKPGDGLFRGRIDHDAFRRAF
                     GSFSRSTVDERMALLGRILALLEERAEQFAQAITTEMGTPIGFARTAHVPFGIAHVRA
                     QIEVLRRYEFLSLEGAFAISKEPIGVCALITPWNWPLYQVTAKVAPAIAAGCTVVLKP
                     SELSPFSARLFAQVMHDAGTPAGVFNLVGGTGEVVGAALAAHPDVDMISITGSTRAGV
                     LVAQAGAVSVKRVVQELGGKSPNLFLDDADFEEGVAKGVLAGMRNAGQSCSAPTRMLV
                     PASRLAEVESLVRAAVAGITLGDPRDEKTSMGPIANAAQYARVQAMIASGIEEGARLV
                     CGGPGRPDGLTRGYFARPTVFSDVTPDMRVAQEEIFGPVLVLMPYRDEAQAIAIANDT
                     VYGLGAAVQSADRERARRVASQLRAGQVHINYPPWNSHAPFGGYKRSGNGREYGVHGL
                     EEYLETKAIVGY"
BASE COUNT          422 a          963 c          939 g          434 t
ORIGIN      
        1 ggaatctgtc ggggtggtag ggacggggat cgacgagcgg ggatgcgccg ctgacgatgt
       61 cggcgatcag gtgcccggcg ccagggccga tgccgaaacc gtggccgctg aagccggcgg
      121 ccagcacgag cccgcgcagg ctcagcattt cgccgatgcc gggcacgccg tcgggcgtgc
      181 tgtccacata gccggcccag gccgcggtca ccgagctgtc cgccaacgcc ggcaccagtt
      241 cgaccgcgcg cctgtacgtc aggtcgacga ccgaggcatc ggccttcgga tcgaggatgc
      301 gcatgcgctc catgggcgtc gtctcgtcga gccgccatcg ggcgaagcct tcatggccgc
      361 agcggatgcc ttcgagtccg ccaggtgcga gattgcgcca gcgccgctgg aacatcggca
      421 ggaactgcgg cgcgaaacgc agcagctgcg gcgtcgggtc cacacgcccc cgtccgctga
      481 tggccagcgt gtagccgccg tcggcgcggc gcgtcatgga cacgccggtc gtgtggagcg
      541 ctgcgggaat gtccttgccc gaggtggtca ccgacagcgc cgtctggcgg atcgcagcct
      601 ggggaaaacg gatgccatat tgacgacaga acgaggatgc ccacgcgccg ccggcgagca
      661 ccgcgacccg ggtgcggatc gtcccgctct cggtgaccac cgcgcagacc tggccgcctt
      721 ctgtttcgag gccgcgcgcc gcgcagccct ggtgcacggt gccgccgagc ttcatgaaag
      781 cccgcgccac ggccggtgcg gcacgcgagg gatcggcggt gccatccgtc ggggcgaaga
      841 cgccgccttt ccatgccttg ccggtggcgt gaccgcgctg cgcggcttca cccgcgctca
      901 gcatgcgcgt ctgcacattc acggagcgtg cgaactcgcc ccagcgcgcc cagccgtcga
      961 gttccttctc gctgttgctg agatagagca gcccgcatct gctgaagccg gtgacttcgc
     1021 cggtgtcctg cgtgaatcgc tcccataggt ccaggctctt cgtggccatc ggcagttcgc
     1081 gcgcatcgcg gttctgctgc cggcaccagc cccagttgcg gctcgactgc tcggcaccga
     1141 tcctgccttt ctcgatcagc gcgacggaaa ccccacgctg agccatgtag taggcggcga
     1201 agacgccgat gatgccgccg ccgatcacga cggcgtcggc atgcgtcggc agttcaggac
     1261 tggattcgac ggcggataga ggcgcaggca ttctgggtat gtcccttgga cgtcagtgaa
     1321 aacttgaagg catgctcgat ggcgatggcg gtcgatgccg gaaagcgatc agtagccgac
     1381 gatcgccttg gtctcgaggt attcctcgag gccgtggacg ccgtactcgc gtccgttgcc
     1441 ggagcgcttg tagccgccga acggcgcgtg gctgttccat ggcggatagt tgatgtgcac
     1501 ctggcccgcg cgcagctgcg aggccacgcg ccgcgcccgc tcgcgatcgg cggactgcac
     1561 ggcggcgccg agtccgtaga ccgtgtcgtt ggcgatggcg atggcctgcg cttcatcccg
     1621 gtagggcatc agcaccagca cggggccgaa tatctcttcc tgcgccacgc gcatgtcggg
     1681 cgtcacgtcc gaaaagaccg tcggtcgtgc gaagtagcca cgggtcaggc cgtccggcct
     1741 gcccgggccg ccgcagacca gcctggcgcc ttcttcgatc ccgctggcga tcatcgcctg
     1801 cacccttgcg tactgcgcgg cgttcgcgat cggccccatc gatgtcttct cgtcgcgcgg
     1861 atcgccgagc gtgataccgg cgaccgccgc gcggaccagc gactccacct cggccagccg
     1921 cgacgcaggc accagcatgc gcgtcggcgc gctgcacgac tggcccgcgt tgcgcatgcc
     1981 tgccagcacg cccttcgcca caccttcctc gaaatcggcg tcgtcgagga acaggtttgg
     2041 cgacttgccg cccagctcct gcacgacgcg cttgacgctg acggcacccg cctgcgcaac
     2101 gagcacgccg gcacgcgtgg agccggtgat ggagatcatg tcgacatcgg ggtgcgcggc
     2161 caacgcagcg ccgaccacct ctccggtgcc gcccaccagg ttgaagacgc cggccggcgt
     2221 gccggcgtcg tgcatcacct gcgcgaagag ccgcgcgctg aacggcgaca gctcgctggg
     2281 cttcaggacg accgtgcagc cggcggcgat cgccggcgcg accttggcgg tgacctggta
     2341 caggggccag ttccacggcg tgatcagcgc gcacacgccg atcggctcct tggagatcgc
     2401 gaaggcgcct tccagagaga gaaactcgta tcgccgcagc acttcgatct gcgcgcgcac
     2461 gtgggcgatc ccgaacggca catgggccgt cctcgcgaac ccgatgggcg tccccatctc
     2521 cgtcgtgatg gcttgcgcga actgctccgc ccgctcttcc agcagcgcga ggatccggcc
     2581 cagcagcgcc atgcgctcgt cgaccgtgct tcgagagaac gagccgaagg cgcggcggaa
     2641 ggcgtcgtgg tcaatccggc cacggaacag gccgtcgccc ggtttcgtct tggaaatgcc
     2701 gaagacgttg tcgacgccgt caccgcagcc cgccgggcct tcgttatctg gagccgaa
//
LOCUS       DATCRO010000246        15214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_36325, whole genome shotgun sequence.
ACCESSION   DATCRO010000246 DATCRO010000000
VERSION     DATCRO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36325"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..633
                     /locus_tag="VIN75_06620"
     CDS             16..633
                     /locus_tag="VIN75_06620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8973873.1"
                     /translation="MDTALPTRAPAPPSDWVVRWTPLLPPASRVLDLACGHGRHVRWL
                     AAAGHRVTAVDRDPALLAPLAGVAEIVQADLEAAPWPLPGRDFDAVVVTNYLWRPLMS
                     AIKGAVVPGGLLIYETFAQAHAALGRPRRPEFLLAPGELIEVLRAPAATVGQACSPSG
                     NWQVIAFEEGRLPARGESPEREVQRIVARRGGAASPGTDLLASPG"
     gene            693..1574
                     /gene="dapA"
                     /locus_tag="VIN75_06625"
     CDS             693..1574
                     /gene="dapA"
                     /locus_tag="VIN75_06625"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY8973874.1"
                     /translation="MERIVGSMVALVTPMHEDGSVDYVALRALVDWHIAEGTDCIVAV
                     GTTGESATVSVEEHVEIIRVCVEHAAGRIPIMAGAGANSTQEAIELSKHAKRVGASCT
                     LQVVPYYNKPSQEGIYRHFAKIAESVDIPVVLYNVPGRTVADMQHDTVVRLAQVPGIV
                     GIKEATGSIERAGWLLRAVPKSFSVYSGDDSTAVALMLMGGHGNISVTANVAPRLMHE
                     LCAAALAGDARRATELHLKLLPLHKNLFCEPSPAPAKFALSRLGRCGPALRLPLVEMT
                     DTGKAIITAALADCGIA"
     gene            1703..2980
                     /gene="bamC"
                     /locus_tag="VIN75_06630"
     CDS             1703..2980
                     /gene="bamC"
                     /locus_tag="VIN75_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY8973875.1"
                     /translation="MTTLPSTRLLHPTPLRVATVTLCLMLGGCTVVGDWFSHGETDYK
                     GASKRTQPLEVPPDLTQLQRDSRYLPQGGVVTASDLNKPAGSGPSQPGAAPAPANPEV
                     ALAAAGNLHLERNGDQRWLTTSDAPEIVLPKVRAYWESQGFTIKTDEPKTGIIETDWK
                     ESRTKLPNDGIRGVLSNLLDSFSDSGLRDRYRTRLERRADGGTEIYVTHFGAVEQSTG
                     RTNSVQTADVEWMPRPTDPGLEATQLSRMMLSLAGLTDTNVAVPPKSGFTFHFGEDDT
                     AASAPASGRNIPSAATSRPADTSGSARARVVAGQPGATMQVDDNFDRSWRRIGLALDH
                     GGFTVEDRDRAQGLYYVRYVDAKEAAKDEPGFFSKFTSWFGKSGPSPVGKYRIQIKAP
                     ADQATVTVLNDQGQPDNSENAQRIVALLVDELK"
     gene            complement(3222..3479)
                     /locus_tag="VIN75_06635"
     CDS             complement(3222..3479)
                     /locus_tag="VIN75_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973876.1"
                     /translation="MNKTLLASLIAVAALAACNKPADQAAPAAADAASAAASSATVAA
                     SAADTSASAAMSAASSATDAASSAATGAMAAASAAATAASK"
     gene            complement(3630..4184)
                     /locus_tag="VIN75_06640"
     CDS             complement(3630..4184)
                     /locus_tag="VIN75_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973877.1"
                     /translation="MNDDAARPQQGPGDDGGLRDAKAAAGGRAFEGAPQFARALHDAM
                     VMASERGTRRLCWCDQDYASWPLGEAEWIDLLTHWSRKPGRELVMIARDWAVVERRHP
                     RFVTWRRDWAHVVQCLVPDEAHTADLPTIWIDSDDQALRVFDPEHLRGRLGFDRVDRQ
                     RAREDFDAISQRASPGFSAVTLGL"
     gene            complement(4177..5355)
                     /locus_tag="VIN75_06645"
     CDS             complement(4177..5355)
                     /locus_tag="VIN75_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8973878.1"
                     /translation="MNLREPLALLGGLSPAAFMRDVWQKRPLLIRNAFPLVGADGAPL
                     SPVTPAQLFELASRDDVESRLVMQDAAPARPKARAKAPSPEGWALKHGPFARRALPPL
                     SQRGWTMLVQGVDLHVQAAHELLQRFRFVADARVDDLMISYASPGGGVGAHIDSYDVF
                     LLQVHGHRRWRVGPVDDPCFVDGLPVRILERFEPIDEWVLGPGDMLYVPPRWGHDGVA
                     EDECMTCSIGFRTPMATELARELLQRVLDDVEADPDEPHFRDPPRSATETPARMPAAL
                     DQFARKAVDRLLKDPSQLARALGEVMTEPKPQVWFESGAERGAGSGLRLDRRTRMMYD
                     DRHVFINGESFNAAGRDARLMRELADARRLVARDCARLSPDAQAVVADWVAQGWAHDE
                     "
     gene            5436..5963
                     /locus_tag="VIN75_06650"
     CDS             5436..5963
                     /locus_tag="VIN75_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8973879.1"
                     /translation="MNITAPCVVSLTWKLSDAQNAPIDELTEPTEFFFGGGDLLPKVE
                     EALDGQAVGYDLQIQLEPEHAFGDYHSELVCFEDRALFPEGVEVGMQFEGLPDGAATP
                     DMPEDAVYTVTEVYPSHVVLDGNHPLAGMALRMHIQVRGVRAATAQEIQDKSVQDDAA
                     GLLQVLPPKDKSQLH"
     gene            complement(6071..6520)
                     /gene="dut"
                     /locus_tag="VIN75_06655"
     CDS             complement(6071..6520)
                     /gene="dut"
                     /locus_tag="VIN75_06655"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY8973880.1"
                     /translation="MTTIDLKVLDARMADALPAYATPGSAGLDLRACLDAPVVLAPGQ
                     TALIPTGLSIHIADPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSCWNRG
                     QAEFTINPMERVAQMVIVPVVQATFRVVEDFDASHRGEGGFGSTGKH"
     gene            complement(6910..8121)
                     /gene="coaBC"
                     /locus_tag="VIN75_06660"
     CDS             complement(6910..8121)
                     /gene="coaBC"
                     /locus_tag="VIN75_06660"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY8973881.1"
                     /translation="MTERKDLAGKHVVLGLSGGIACYKAADLARLLGKAGATVQVVMT
                     AGAEQFITPVTMQALTGRPVYASQWDARPDNNMAHINLSREADAILVAPASAEFMAKL
                     VHGRADDLLSLLCLARPIDRVPLLVAPAMNREMWAHPATQRNVTQLQADGATLLGPDV
                     GEQACLEFGEGRMLEAADLRDELIAFFQPKILRGRRILVTAGPTFEAIDPVRGITNHS
                     SGKMGFAIARAAQEAGADVTLVAGPVHLPTPRGVRRVDVQSARQMHEAVLPLAQASDV
                     FVATAAVADWRPAQMNEHKIKKDGKKVAPTFELTENADILADVARLSPRPYCVGFAAE
                     SENLLKHAREKLQRKNVPLIVGNLGPATFGRDDNALVLVDAKGERELPNADKLTLARQ
                     LVAEIAMRIAA"
     gene            complement(8351..9142)
                     /locus_tag="VIN75_06665"
     CDS             complement(8351..9142)
                     /locus_tag="VIN75_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY8973882.1"
                     /translation="MTTPFTHLVHYPTNTDADLDRLQQLCEQLAGFDEHVSLEWLDGA
                     MTALAAGPRALTVMDWRDRLLGDSWSRAFGDPEADREATAVLQARWTVLLKQLDPELL
                     MDAVDELRLAPVMLEWTDEDKARLVEDGTIAPGTEDEEIPLTGEVWAHGFMDVLQNFK
                     DEWPEQDVETDTGQLFDETTMRIVMLTLHDQAELDKLREEMFPGEKLERDDLIQEALF
                     AVQDLRCYWVQNAPKPATRVAEKLPGRNDPCFCGSGKKFKKCHGA"
     gene            complement(9205..9774)
                     /locus_tag="VIN75_06670"
     CDS             complement(9205..9774)
                     /locus_tag="VIN75_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973883.1"
                     /translation="MSRPEATADFAAPPGVTVRRTRVTDAAAFATMMDHPDVFPNLLQ
                     MPHASEEHWKARLTESAGAGSPHVQIVAEADGEVVGAAGLHPIGTSPRKRHSMLLGIN
                     VAAGWQGRGVGKLLMHSLCDYADNWLGLLRIELDVFADNKRAQALYERFGFVREGVRR
                     CEALRDGVYVDGWLMARLHPSPLRGFPAA"
     gene            complement(10076..10216)
                     /locus_tag="VIN75_06675"
     CDS             complement(10076..10216)
                     /locus_tag="VIN75_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973884.1"
                     /translation="MPLAPKPLIRRAVALRALAGGRSPSWLRPQLRALRRIVFAKPSR
                     SR"
     gene            complement(10388..13021)
                     /gene="alaS"
                     /locus_tag="VIN75_06680"
     CDS             complement(10388..13021)
                     /gene="alaS"
                     /locus_tag="VIN75_06680"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8973885.1"
                     /translation="MKAAEIRSTFLKFFESKGHTIVASSPVVPGDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFK
                     KDAISYAWELLTVHFKLPVDKLWVTVYAEDDEAYEIWNKTVGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGPEIPGGPPGSPEEDGDRYIEIWNNVFMQFNRT
                     EDGVMHKLPKPSVDTGMGLERLAAVLQHVHSNYEIDLFVNLLAAAKKAVDEATPNGGD
                     CDKESPSLKVIADHIRACSFTVVDGVIPGNAGRGYVLRRIARRAIRHGYKLGARTPFF
                     HKLVAALSAEMGEAYPELRAAQARVTEVLQQEEERFFRTIATGMELLESALAGGAKQV
                     DGETAFKLHDTYGFPVDLTGDVCRERGVTVDEAGFNRLLEEQKERGRQAGKFKMAQGL
                     AYSGAPTQFHGYERLVVETVKVVGVYVDGNPVNSATAGDDAVVVLDQTPFYAESGGQV
                     GDTGELRNATTRMLVEDTLKIQADVFGHHGRIVEGTINVGDSFVAKVDVERRARTVRN
                     HSATHLMHKALREVLGAHVQQKGSLVNPERTRFDFAHTGPMTAEQIRKVEEIVNAEVL
                     ANAAAKAQVMAIDDAQKSGAMMLFGEKYGDQVRVLTIGSSKELCGGTHVERTGDIGLF
                     KIVAESGVAAGIRRVEAVTGANALAYLQSLESTVDEVASTLKASPEEVPARLAHVLDH
                     VKALEKELEKLKARLASAQGDELVSKAVDIKGLKVLAARLDGADAKALRETMDKLKDK
                     LKTAAIVLAAVDGDKVQIAAGVTADSIGRIKAGELVNFVAQQVGGKGGGKPDMAMAGG
                     TDASRLPGALASVQGWVQERA"
     gene            13229..14104
                     /locus_tag="VIN75_06685"
     CDS             13229..14104
                     /locus_tag="VIN75_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973886.1"
                     /translation="MPDLPPAPPPHELPADAPPSSAPWVSTQTEMETAARPQRTRVFR
                     VRMKTGHVRVAGVTYFQATQSSATDSRVEHLYRVQLSWLAEFDPRDGKSQRFAERTST
                     GRATNALQFRVLEMSTDGKRVSAARLGPKGQILAEPTGMGVMSLLRGLLVEWVAALHP
                     DATVLTGSLYQVDVPSEKEMELRDAFFARSGFTVRTTSGGGGSFFARSIRELKTTWNT
                     DKVTELTAPLLAEAVSAQMEMPLLRQQIASLQDQLTTAIKEKRATEVLSRVWLAITVL
                     AVIFGLVFGIQPRLG"
     gene            complement(14231..>15214)
                     /locus_tag="VIN75_06690"
     CDS             complement(14231..>15214)
                     /locus_tag="VIN75_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8973887.1"
                     /translation="ENVELDGYGDPRGHLPLREWIRDAMAEAEIALQPDQVLLTHGSS
                     HALDLAIRRLVRAGDAVLVDDPGYSNELFALRVAGARLIPVPRTPQGYDLAALEEAIV
                     THHPRVFFTQPRLQSPTTSTATLPQLHRVLQLADRHGLTIVENDLYVDLDPDPRPSLA
                     SLDQLARVLYVGSYSKTISPNLRVGYIAAHPALIDDLAQLKMISGLTSSAFSERLILG
                     ALTEGRWRKHLKSLRARLAEAHETCGQRLVALGFELFHEPKAGMFLWARHPRLADPVA
                     LSNRAAQHDIMLGPGHLFSATLQPTDWMRFNVAFAGDVRLGAFLEAELAGG"
BASE COUNT         2246 a         5335 c         5328 g         2305 t
ORIGIN      
        1 ccttagagtg gcggcatgga caccgccctg cccacccgcg ccccggcgcc gccttccgac
       61 tgggtcgtac gctggacgcc cttgctcccg cctgcctccc gcgtgctgga cctcgcctgc
      121 ggccacggcc ggcacgtgcg ctggctggcc gcggccggcc atcgcgtcac cgccgtcgat
      181 cgcgacccgg ccctgctcgc gccgctggcc ggcgtcgccg agatcgtgca ggcggacctc
      241 gaggcggctc cgtggcccct gccgggacga gatttcgacg cggtcgtcgt caccaactac
      301 ctgtggcgcc ccctcatgtc ggcgatcaag ggcgccgtgg tgcctggcgg actgctgatt
      361 tacgaaacct ttgcacaagc gcacgcagca ctcggacggc cgcgaaggcc cgaattcctg
      421 ctcgcgccgg gggagttgat cgaggtcctg cgcgcgcctg cggcgacggt cgggcaggcc
      481 tgttctccct ctggaaactg gcaggtgatc gcgttcgagg aaggcaggct gcccgcccgc
      541 ggcgagagcc ccgaacgcga ggtccaacgc atcgtcgcgc ggcgtggcgg cgcggcatcg
      601 ccgggcaccg accttctggc gtcgccgggc taaaatcgtc ggattgccgg cgtcgcgcgc
      661 gtccggcccc cggaacctgg cgaggaatgc gaatggaaag aattgtcggc agcatggtgg
      721 cgctggtcac ccccatgcac gaggacggca gcgtcgacta tgtcgcgctg cgcgcgttgg
      781 tcgactggca catcgccgag ggcaccgact gcatcgtcgc cgtgggcacc accggcgaat
      841 cggccacggt cagcgtcgag gagcacgtcg agatcatccg cgtgtgcgtc gagcatgccg
      901 ccggccgcat ccccatcatg gcgggcgcgg gggccaactc cacgcaggag gccatcgagc
      961 tgtcgaagca cgccaagcgc gtgggcgcct cctgcacgct gcaggtcgtg ccgtactaca
     1021 acaagccctc gcaggaaggc atctaccggc acttcgccaa gatcgcggag tcggtggaca
     1081 tccccgtggt gctgtacaac gtgccgggcc gcaccgtggc cgacatgcag cacgataccg
     1141 tggtgcgcct cgcccaggtg cccggcatcg tgggcatcaa ggaagccacc ggcagcatcg
     1201 agcgcgcggg ctggctgctg cgcgcggtgc ccaagtcgtt ctcggtctat tcgggcgacg
     1261 acagcaccgc tgtcgcgctg atgctcatgg gcggccacgg caacatcagc gtcaccgcca
     1321 acgtcgcgcc gcgcctgatg cacgagctgt gcgccgcggc gctggccggt gacgcccgcc
     1381 gcgccacgga gctgcacctg aagctgctgc cgctgcacaa gaacctgttc tgcgagccga
     1441 gcccggcgcc cgccaagttc gcgctgtcgc gcctgggccg ctgcggtccc gcgctgcggc
     1501 tgccgctggt ggagatgacc gacaccggca aggcgatcat caccgccgcc ctcgccgact
     1561 gcggcatcgc ctgagccgcc cgccccgtca ccgactcgag ccggtggcct gcctgcgccg
     1621 gcgttccccg gcccgcgacc ctcgcgacct ctgcgacaat ccccggcccg gaacttccgg
     1681 gcagacctcg accctgatgc acatgaccac cctgccctcg acgcgcctgc tccatcccac
     1741 gcccctgcgc gttgccaccg tgacgctgtg cctgatgctg ggcggatgca cggtcgtcgg
     1801 agactggttc tcccacggcg agaccgacta caagggcgcg tccaagcgca cccagccgct
     1861 ggaagtgccg cccgacctga cgcagctgca gcgcgattcg cgctacctgc cgcaaggcgg
     1921 cgtggtcacc gcctccgacc tgaacaagcc ggccggcagc gggccgtcgc agccgggcgc
     1981 agcgcccgct ccggccaacc ccgaggtggc gctggccgcc gccggcaacc tgcacctgga
     2041 gcgcaacggc gaccagcgct ggctgacgac gtcggacgcg cccgagatcg tgctgcccaa
     2101 ggtgcgcgcc tactgggagt cgcagggttt caccatcaag accgacgaac ccaagacggg
     2161 catcatcgag accgattgga aggaaagccg caccaagctg ccgaacgacg gcatccgcgg
     2221 cgtcctgtcg aacctgctgg acagcttctc cgattcaggc ctgcgcgatc gctatcgcac
     2281 gcgcctggag cggcgcgccg acggcggcac cgagatctac gtgacccact tcggcgccgt
     2341 cgaacagtcc acgggccgga ccaacagcgt gcagaccgcc gacgtggaat ggatgccgcg
     2401 tccgacggat ccgggcctcg aggccacgca gctgagccgg atgatgctga gcctggccgg
     2461 cctgaccgac accaacgtcg ccgtgccgcc caagagcggc ttcacgttcc acttcggcga
     2521 ggacgacacc gccgccagcg cgcccgcgag cggccgcaac atccccagcg ccgccaccag
     2581 ccgcccggcc gacaccagcg gcagcgcccg cgctcgcgtc gtcgccggcc agcccggcgc
     2641 gacgatgcag gtcgacgaca acttcgatcg ctcgtggcgc cgcatcggcc tggcactcga
     2701 ccacggcggc ttcaccgtgg aagaccgcga ccgcgcgcaa ggcctgtact acgtgcgcta
     2761 tgtcgacgcc aaggaagcgg ccaaggacga gccgggcttc ttctcgaagt tcacgtcgtg
     2821 gttcggcaag agcggcccgt cgccggtggg caagtaccgc atccagatca aggcgcccgc
     2881 cgaccaggcc accgtcaccg tgctgaacga ccagggccag cccgacaaca gcgagaatgc
     2941 ccagcgcatc gtggcgctgc tggtcgacga gctgaagtaa gcacggcatc cgcatcgcgc
     3001 agcaaaaggc cgcccgaggg cggcctttgt cgttctggct tagggtgcga cctgtcgacg
     3061 cgtcaatccc cttgtgacct cttgacaggg ttgccaagcc attcgctccc gcattccagg
     3121 gattcaactt gccttcagcg acctgacatg aaaaagccgc ccgaaggcgg ctcttcatat
     3181 ctcgggacac cgcgctcgcg aggagcgcgg agatccggaa attacttgct ggcagcggtc
     3241 gcggcggccg aagcagcagc catggcgccc gtggcggccg acgaagcggc gtcggtggcc
     3301 gacgaagcgg cagacatggc ggcggaggcc gaggtgtcag cggccgaagc ggcgacggtg
     3361 gcggacgaag cggcagccga agcggcgtcg gcagcggccg gagcagcttg gtcggccggc
     3421 ttgttgcaag cggccagggc ggcgacggcg atcagagagg ccaggagcgt cttgttcatg
     3481 aatttcctcg aagaaaaatt ttgggttcga accattaccg gtaatccaga tactcgccct
     3541 cacggcaggt atctgagcaa aagacagcag gcaagctcga gcatttcctg tgcctagccc
     3601 gccattatac ccatcaatac ggaacctttt cagagtccca gtgtcaccgc cgaaaaaccg
     3661 gggctggcgc gttgtgaaat cgcatcaaag tcctcccgcg cccgttggcg atcgacgcgg
     3721 tcgaagccga ggcggccgcg cagatgttca gggtcgaaga cgcgcagcgc ctggtcatcg
     3781 gagtcgatcc agatcgtggg caggtcggcc gtgtgcgctt cgtcgggcac gaggcactgc
     3841 acgacatgcg cccagtcgcg gcgccacgtg acgaagcgcg gatggcggcg ttcgacgacg
     3901 gcccagtcgc gcgcgatcat cacgagttcg cgcccgggct tgcgcgacca gtgcgtgagc
     3961 aggtcgatcc actccgcctc gcccaggggc cagctcgcgt aatcctggtc gcaccagcac
     4021 aggcggcgcg tgccgcgctc gctggccatc accatcgcgt catgcagcgc gcgcgcgaac
     4081 tgcggcgcgc cctcgaacgc gcggccgccc gcggcagcct tcgcatcgcg cagccccccg
     4141 tcgtcgcccg gcccttgctg cggccgcgcc gcgtcgtcat tcatcatgtg cccatccctg
     4201 cgccacccaa tcggccacga cggcctgcgc gtcaggcgac aggcgggcac agtccctggc
     4261 caccagtcgc cgcgcgtcgg ccagctcgcg catcaggcgc gcatcgcggc cggccgcgtt
     4321 gaacgactcg ccgttgatga agacgtgccg atcgtcgtac atcatgcgcg tgcgccgatc
     4381 gagccgcaag ccgctgcccg cgccgcgttc cgcgcccgac tcgaaccaca cctgcggctt
     4441 gggctcggtc atcacctcgc cgagcgcgcg cgccagctgc gacggatcct tcagcaggcg
     4501 atcgaccgcc ttgcgcgcga actggtcgag cgcggcgggc atgcgcgcgg gcgtctcggt
     4561 ggcgctgcgc ggcggatcgc ggaaatgcgg ctcgtcgggg tcggcctcga cgtcgtcgag
     4621 cacgcgctgc agcagctcgc gtgcgagctc ggtggccatc ggggtgcgaa agccgatcga
     4681 gcacgtcatg cactcgtcct cggccacgcc gtcgtgaccc cagcgcggcg gcacgtagag
     4741 catgtcgccg gggcccagca cccattcgtc gatcggttcg aagcgttcga ggatgcgcac
     4801 cggcaggccg tcgacgaagc acgggtcgtc gaccggcccg acgcgccagc gccggtggcc
     4861 gtgcacctgc agcaggaaca cgtcgtacga atcgatgtgc gcgccgacgc cgccgcccgg
     4921 gctcgcgtac gagatcatga ggtcgtcgac gcgcgcgtcg gcgacgaagc gaaagcgttg
     4981 cagcagctcg tgcgcggcct gcacgtgcag gtcgacgccc tgcaccagca tcgtccagcc
     5041 gcgctgcgac agcggcggca gcgcgcggcg tgcgaacggg ccgtgcttca gcgcccagcc
     5101 ttcgggcgac ggcgccttcg cgcgcgcctt cggcctggcg ggcgccgcgt cctgcatcac
     5161 gagccgcgat tcgacgtcgt cgcgcgacgc cagctcgaac agctgcgccg gcgtgacggg
     5221 cgacagcggc gcgccgtccg cgcccaccag cgggaaggcg ttgcggatca gcagcggacg
     5281 cttctgccag acgtcgcgca tgaaggccgc gggcgacagg ccgcccagca gcgcgagggg
     5341 ttcacggagg ttcatcgcgc cattgtccct gctcgcggac cggggcgctg cggcgcgggc
     5401 gcgtggcggt cacgcgcgcc tcgtaaaatt cgccgatgaa catcaccgcc ccttgcgtcg
     5461 tctcgctcac ctggaagctc tcggacgccc agaacgcgcc catcgacgag ctcaccgagc
     5521 ccaccgagtt cttcttcggc ggcggcgacc tgctgcccaa ggtcgaagag gcgctcgacg
     5581 gccaggccgt cggctacgac ctgcagatcc agctcgagcc cgagcacgcc ttcggcgact
     5641 accattccga gctggtctgc ttcgaggatc gcgcgctgtt ccccgagggc gtcgaggtgg
     5701 gcatgcagtt cgagggcctg cccgatggcg ccgccacgcc cgacatgccc gaggacgccg
     5761 tctacacggt caccgaggtc tatccgtcgc acgtggtcct ggacggcaac cacccgctgg
     5821 ccggcatggc gctgcgcatg cacatccagg tgcgcggcgt gcgcgccgcg acggcgcagg
     5881 agatccagga caagagcgtg caggacgatg ccgccgggct gctgcaggtg ctgccgccga
     5941 aggacaagtc gcaacttcac tgaggcgcgc ctcgagaaga cgcgcttccc tgcggatcgg
     6001 cgtcaggcac ggcctcaagc cgctggcgcg atccgacgag gggccagccc cctgcgcgcc
     6061 gggccgctgc tcagtgcttg cccgtcgatc cgaacccgcc ctcgccgcgg tgcgacgcgt
     6121 cgaagtcctc gacgactcgg aacgtcgcct gcaccaccgg cacgatcacc atctgcgcga
     6181 cgcgctccat cggattgatc gtgaactccg cctgtccgcg gttccagcag ctgaccatca
     6241 gctggccctg gtagtccgag tcgatcaggc ccacgaggtt gcccagcacg atgccgtgct
     6301 tgtggcccag gcccgagcgc gggaggatca tcgcggcgag accggggtcg gcgatgtgga
     6361 tggacaggcc ggtggggatc agcgcggtct ggccgggcgc aaggacgacg ggggcgtcga
     6421 ggcaggcgcg caggtcgagg ccggcgctgc ccggcgtggc gtaggccggc agggcgtcgg
     6481 ccatgcgggc gtcgagaacc ttgaggtcga tggtggtcat gggcgggatg tcgtcgaggg
     6541 aaatggatgg ccgggtcggt cagcgggacg gccgatgcgg cgtccgggcc cgatcttgcg
     6601 atgtggcgcg ttcgatcgag tatcgatggc gtcttgcgac ggggtcaggt cttgcgacgg
     6661 ggtcaggtct tgaaagttgc gcgatcgtag cgacggggtc aggtcttgaa agttgcgcgg
     6721 cagtgccccg gcgatcggct gagacgtcat cgcgcaactt tcaagacctg accccacggc
     6781 gaggcgtcgc cacgcaattt tcaagacctg accccacggc gaggcgtcgc cacgcaactt
     6841 tcaagacctg accccgtcac cagaccccgt cgccacgatg cgtccgccaa gccgccgcgc
     6901 cacgcatgat caagcggcga tgcgcatcgc gatctccgcc acgagctgcc gcgccagcgt
     6961 gagcttgtcg gcgttgggca gctcgcgctc gcccttcgcg tcgaccagca ccagcgcgtt
     7021 gtcgtcgcgg ccgaaggtgg cggggccgag gttgccgacg atcagcggca cgttcttgcg
     7081 ctgcagtttc tcgcgcgcgt gcttgagcag gttctcgctc tcggcggcga agcccacgca
     7141 gtagggccgc ggcgacaggc gcgcgacgtc ggcgaggatg tcggcgttct cggtcagctc
     7201 gaaggtgggc gccaccttct tgccgtcctt cttgatcttg tgctcgttca tctgcgcggg
     7261 acgccaatcg gcgacggccg cggtggcgac gaacacgtcg ctcgcctgcg cgagcggcag
     7321 cacggcctcg tgcatctggc gcgcgctctg cacgtcgacg cggcgcacgc cgcgcggcgt
     7381 gggcaggtgc accggaccgg cgacgagcgt gacgtccgcg cccgcttcct gcgccgcgcg
     7441 cgcgatcgcg aagcccatct tgccgctgga atggttggtg atgccgcgca ccgggtcgat
     7501 ggcctcgaag gtggggccgg ccgtgacgag gatgcgcctg ccgcgcaaaa tcttgggctg
     7561 gaagaaggcg atcagctcgt cgcgcaggtc ggccgcctcg agcatgcggc cctcgccgaa
     7621 ctcgaggcac gcctgctcgc cgacatcggg gcccagcagc gtggcgccat cggcctgcaa
     7681 ttgggtgacg ttgcgctgcg tggccggatg ggcccacatc tcgcggttca tcgccggcgc
     7741 caccagcagc ggcacgcggt cgatcgggcg cgccaggcac agcagcgaca gcaggtcgtc
     7801 ggcgcgcccg tgcaccagct tggccatgaa ctcggcgctg gccggcgcga cgaggatggc
     7861 gtcggcctcg cgcgacaggt tgatgtgcgc catgttgttg tcggggcgcg cgtcccactg
     7921 gctcgcgtag acggggcggc ccgtcagcgc ctgcatcgtc accggcgtga tgaactgctc
     7981 cgcgccggcg gtcatcacca cctgcaccgt ggcgccggcc ttgcccagca ggcgcgcgag
     8041 gtcggcggcc ttgtagcagg cgatgccgcc agagaggccc agcacgacgt gcttgcctgc
     8101 gagatccttg cgttcagtca tgggcgcaat gtaatgcctt atccggttcg actgtgcggg
     8161 ccgggcgttt cttccgggtt cgatcctgcg actgcgctcc gctgcgcgca ggatgacggc
     8221 ttgcgagacg gccggtgccg tcagcccgtc acgccgtcgt gccgccaagg tgtcatcctg
     8281 cgtgaagtcg caggatccac gcaccgcgcg gcgccgccct gcgcggacaa cgaccagcgc
     8341 cagcgcggac tcacgcgccg tggcacttct tgaacttctt gccgctgccg cagaagcacg
     8401 gatcgttgcg tcccggcagc ttctcggcga cgcgcgtcgc cggcttcggg gcgttctgca
     8461 cccagtagca acgcaggtcc tgcacggcga acagcgcctc ctggatcagg tcgtcgcgct
     8521 cgagcttctc gcccgggaac atctcctcgc gcagcttgtc gagctcggcc tggtcgtgca
     8581 gcgtgagcat gacgatgcgc atcgtcgtct cgtcgaacag ctggccggtg tcggtctcga
     8641 cgtcctgctc gggccattcg tccttgaagt tctgcagcac gtccatgaag ccgtgggccc
     8701 agacctcgcc ggtgagcggg atctcctcgt cctcggtgcc gggggcgatg gtgccgtcct
     8761 cgacgagcct ggccttgtcc tcgtccgtcc attccagcat gaccggcgcg aggcgcagct
     8821 cgtcgacggc gtccatcagc agctccggat cgagctgctt gagcagcacg gtccagcgcg
     8881 cctgcagcac ggcggtggcc tcgcggtcgg cctcggggtc gccgaacgcg cggctccacg
     8941 agtcgcccag caggcggtcg cgccagtcca tcaccgtcag cgcgcgcggg cccgccgcca
     9001 gggcggtcat cgcgccgtcc agccattcca gcgacacgtg ctcgtcgaag ccggcgagct
     9061 gctcgcacag ctgctgcagt cgatcgaggt cggcgtcggt gttggtcggg tagtggacga
     9121 ggtgcgtgaa cggcgtggtc atggggcgca gggcgaaggc ggaagcggcc ggcattttct
     9181 catgccggcc gctcccggcc tgcttcacgc cgccgggaag ccgcgcaagg gcgacgggtg
     9241 caggcgcgcc atcagccagc cgtcgacgta cacgccgtcg cgcagcgcct cgcagcgccg
     9301 cacgccctcg cgcacgaagc cgaagcgctc gtagagtgcc tgcgcgcgct tgttgtcggc
     9361 gaagacgtcc agctcgatgc gcagcaggcc cagccagttg tcggcgtagt cgcacagcga
     9421 atgcatcagc agcttgccga cgccgcggcc ctgccagccg gcggccacgt tgatgcccag
     9481 cagcatcgaa tggcgcttgc gcggcgacgt gccgatcgga tgcaggccgg cggcgccaac
     9541 gacctcgccg tcggcttcgg ccacgatctg cacgtgcggc gagccggcgc cagcgctctc
     9601 ggtcaggcgc gccttccagt gctcctcgct ggcgtgcggc atctgcagca ggttcgggaa
     9661 tacgtcgggg tggtccatca tcgtcgcgaa ggccgcagcg tcggtgacgc gcgtgcggcg
     9721 cacggtcacg cccggcgggg ccgcgaaatc ggcggtggct tcaggtcggc tcatgaacat
     9781 ctcctcgtgg gggtgcctgg gtcggcggct gcaagaagca caaggcccgc ggatggcggg
     9841 ccttgtggaa gaaatcagag atattgaagt caggaacggc ccgggccccg acttcgcgcg
     9901 atgccagtga cgacgccttg cagatgcatc gtcatggccg cggcatacgc catcgcgcct
     9961 ggcgcgcacg acgccgcgcc cacgcgctcg gcggcgtggg cgatgctggc gaagtgggag
    10021 gcgcgggtca tgcgacccag tctgcgggca aacggccgca gcgtcaaggc gacgttcagc
    10081 gcgaacgcga cggcttggcg aagacgatgc ggcgcagcgc gcgcagctgt ggccgcagcc
    10141 acgaaggcga gcggccgccg gcgagcgcgc gcagcgcgac ggcgcggcgg atcagcggct
    10201 tgggggcgag cggcatggac ccagtctggg cgcgggccgc ggggtcgtca agacgggcgg
    10261 aggctatccg ccgcggcgcg cgatcatgcc aaacgatcgc gcgctggggc gcgtgaggcg
    10321 gacacgcgag cgcgaggacg cgcatgacgc gcatgacgcg catgacgcgc atgacgcgcg
    10381 cgacgcgtca ggcgcgttcc tgcacccagc cctgcacgct ggcgagcgcg ccgggcagcc
    10441 tggaggcatc ggtgccgccg gccatggcca tgtccggctt gccgccgccc ttgccgccga
    10501 cctgctgcgc cacgaagttg acgagctcgc cggccttgat gcggccgatc gagtcggcgg
    10561 tgacgccggc cgcgatctgc accttgtcgc cgtcgacggc ggccagcacg atggccgcgg
    10621 tcttgagctt gtccttgagc ttgtccatcg tctcgcgcag cgccttggcg tcggcgccgt
    10681 cgagcctggc ggccagcacc ttcaggccct tgatgtcgac ggccttggac acgagctcgt
    10741 cgccctgcgc cgaggcgagc ctggccttga gcttctcgag ttccttctcg agcgccttga
    10801 cgtggtcgag cacgtgcgcg aggcgcgcgg gcacttcctc gggcgaggcc ttcagcgtgg
    10861 aggcgacctc gtcgaccgtg ctctcgagcg actgcaggta cgcgagcgcg ttcgcgccgg
    10921 tgaccgcctc gacgcggcgg atgccggccg cgacgcccga ctcggcgacg atcttgaaca
    10981 ggccgatgtc gcccgtgcgc tcgacgtgcg tgccgccgca cagctccttg ctggagccga
    11041 tggtcagcac gcgcacttgg tcgccgtact tctcgccgaa cagcatcatc gcgcccgact
    11101 tctgcgcgtc gtcgatggcc atcacctgcg ccttggcggc cgcgttggcc agcacctcgg
    11161 cgttgacgat ctcctcgacc ttgcggatct gctcggcggt catcgggccg gtgtgcgcga
    11221 agtcgaagcg cgtgcgctcc gggttcacga gcgagccctt ctgctgcacg tgcgcgccga
    11281 gcacctcgcg cagcgccttg tgcatcaggt gggtggcgct gtggttgcgc acggtcctgg
    11341 cgcggcgctc cacgtcgacc ttcgcgacga acgagtcgcc gacgttgatc gttccctcga
    11401 cgatgcggcc gtggtggccg aacacgtccg cctggatctt gagggtgtcc tcgaccagca
    11461 tgcgcgtggt ggcgttgcgc agttcgccgg tgtcgcccac ctggccgccg ctttccgcgt
    11521 agaacggggt ctggtcgagc acgacgacgg cgtcgtcgcc ggccgtcgcc gagttcaccg
    11581 ggttgccgtc gacatagacg ccgaccacct tgacggtctc gaccaccagg cgctcgtagc
    11641 cgtggaactg cgtgggcgcg ccgctgtagg ccaggccctg cgccatcttg aacttgccgg
    11701 cctggcggcc gcgttccttc tgttcctcga gcaggcggtt gaagcccgcc tcgtcgaccg
    11761 tcacgccgcg ctcgcggcag acatcgcccg tcaggtcgac cgggaagccg taggtgtcgt
    11821 gcagcttgaa cgccgtctcg ccgtcgacct gcttcgcgcc gccggccagc gccgactcca
    11881 gcagttccat gccggtggcg atggtcctga agaagcgctc ctcttcctgc tgcagcactt
    11941 ccgtgacgcg cgcctgtgcc gcgcgcaact cgggataggc ctcgcccatc tcggccgaca
    12001 gcgcggccac cagcttgtgg aagaacggcg tgcgcgcacc cagcttgtag ccgtggcgga
    12061 tggcgcggcg cgcgatgcgg cgcagcacgt agccgcggcc cgcgttgccc gggatcacgc
    12121 cgtcgacgac ggtgaacgag caggcgcgga tgtggtcggc gatgaccttg agcgagggcg
    12181 actccttgtc gcagtcgccg ccgttcggcg tcgcttcgtc gacggccttc ttggccgcgg
    12241 cgagcaggtt cacgaacagg tcgatctcgt agttcgagtg cacgtgctgc agcacggcgg
    12301 ccaggcgctc caggcccatg ccggtgtcga cgctgggctt gggcagcttg tgcatcacgc
    12361 cgtcttcggt gcggttgaac tgcatgaaga cgttgttcca gatctcgatg tagcggtcgc
    12421 cgtcctcttc cggcgagccc ggggggccgc ccgggatctc ggggccgtgg tcgtagaaga
    12481 tctcggtgca cgggccgcag gggccggtgt cgcccatcat ccagaagttg tccgacgcgt
    12541 agcgcgcgcc cttgttgtcg ccgatgcgca cgatgcgctc ggcgggcacg cccaccgtct
    12601 tgttccagat ctcgtaggcc tcgtcgtcct cggcgtagac cgtcacccac agcttgtcga
    12661 cgggcagctt gaagtgcacc gtcagcagct cccaggcgta gctgatcgcg tccttcttga
    12721 agtagtcgcc gaacgagaag ttgcccagca tctcgaagaa cgtgtggtgg cgcgcggtgt
    12781 agccgacgtt gtcgaggtcg ttgtgcttgc cgccggcgcg gatgcacttc tgcgaggtgg
    12841 tggcgcgcgt gtacgggcgc ttgtcgaagc ccaggaacac gtccttgaac tggttcatgc
    12901 ccgcgttcgt gaacagcagg gtcggatcgt cgcccggcac caccggcgac gaggcgacga
    12961 tggtgtggcc cttcgattcg aagaacttca ggaaggtcga acggatttct gcggctttca
    13021 ttccggcact ttctgggagc ctcgccgtgg attcacctgg gcgggcagca ggcgcacgac
    13081 gaggtcgcgc ggaaccgtcg attataggag gcggggtcgg cacgcccgct gcctgcggga
    13141 atcggtacaa cgtcaccgcc ccggggcatt cctcccttgc cgcggcccgg cgccggctca
    13201 caatgggtcg atccctttcc cccctgccat gcccgatctc ccgcccgcgc cgccgcccca
    13261 cgaactgccc gccgacgccc cgccctcgtc cgcgccctgg gtcagcacgc agaccgagat
    13321 ggagaccgcc gcgcggccgc agcgcacgcg cgtgttccgc gtgcgcatga agacgggcca
    13381 cgtgcgcgtg gccggcgtca cgtacttcca ggcgacgcag tccagcgcca ccgacagccg
    13441 cgtcgagcac ctgtaccgcg tgcaactgag ctggctggcc gagttcgacc cgcgcgacgg
    13501 caagtcgcag cgcttcgctg aacggacgtc cacggggcgg gcgaccaacg cgctgcagtt
    13561 ccgcgtgctc gagatgagca ccgacggcaa gcgcgtcagc gccgcccgcc tgggccccaa
    13621 gggccagatc ctggccgagc cgaccggcat gggcgtcatg agcctgctgc gcggcctgct
    13681 cgtcgaatgg gtcgccgcgc tccaccccga cgcgacggtg ctgaccggct cgctctacca
    13741 ggtcgacgtg cccagcgaga aggagatgga gctgcgcgac gccttcttcg cgcgctcggg
    13801 cttcacggtg cggaccacgt cgggcggcgg cggcagcttc ttcgcgcgct cgatccgtga
    13861 attgaagacc acgtggaaca ccgacaaggt gacggagctc accgcgccgc tgctggccga
    13921 ggccgtcagc gcgcagatgg agatgccgct gctgcgccag cagatcgcgt cgctgcagga
    13981 ccagctgacc accgcgatca aggagaagcg cgcgaccgag gtgctgtcgc gcgtgtggct
    14041 ggcgatcacg gtgctggcgg tgatcttcgg gctggtgttc gggatccagc cgcgcttggg
    14101 gtagccgacc tcgccgtcat cctgtgcgga gcgaagcgga gtcgcaggat ccatcgtggc
    14161 gaaggcgctc ggtccgcgaa ggtggatcct gcgactgcgc gcaggatgac atcgcacggc
    14221 gctggcgcct tcagccgccc gccagctccg cctccaggaa cgccccgagg cgtacatcgc
    14281 ccgcgaacgc cacgttgaag cgcatccagt cggtgggctg caacgtcgcg gaaaacaggt
    14341 gccccggccc cagcatgatg tcgtgctgcg cggcccggtt cgacaacgcg accgggtccg
    14401 ccagccgcgg atgccgcgcc cacaggaaca tgccggcctt gggctcgtgg aacagctcga
    14461 agccgagcgc gacgaggcgc tggccgcagg tctcgtgcgc ctcggccagg cgggcgcgca
    14521 ggctcttgag atgcttgcgc cagcggcctt cggtcaatgc gccgaggatc aggcgctcgc
    14581 tgaaggccga cgaggtcagg cccgagatca tcttcagctg cgcgaggtcg tcgatcaacg
    14641 ccggatgcgc ggcgatgtag cccacgcgca ggttgggcga gatcgtcttg gagtagctgc
    14701 cgacgtagag cacgcgcgcc agctggtcga ggctggccag tgacgggcgc gggtcggggt
    14761 cgaggtcgac gtagaggtcg ttctcgacga tggtcaggcc gtgccggtcg gccagctgca
    14821 gcacgcggtg cagctgcggc agcgtggcgg tggatgtcgt cgggctctgc aggcgcggct
    14881 gcgtgaagaa cacgcgcggg tggtgcgtga cgatggcctc ctccagcgca gcgaggtcgt
    14941 agccctgcgg cgtgcgcggc acggggatca gccgcgcgcc cgccacgcgc agcgcgaaca
    15001 gctcgttgga atagccgggg tcgtcgacca gcaccgcgtc gccggcgcgc acgaggcggc
    15061 ggatggcgag gtccagcgcg tggctggagc cgtgcgtcag cagcacctgg tcgggctgca
    15121 gcgcgatctc ggcctcggcc atcgcgtcgc ggatccactc gcgcagcggc aggtggccgc
    15181 gcgggtcgcc gtagccgtcg agctcgacgt tctc
//
LOCUS       DATCRO010000247         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_58972, whole genome shotgun sequence.
ACCESSION   DATCRO010000247 DATCRO010000000
VERSION     DATCRO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58972"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VIN75_06695"
     CDS             <1..310
                     /locus_tag="VIN75_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973888.1"
                     /translation="TNVYAGRLDGVASITSLSHSAQNQEAVLLEGAPGLALPTSYERF
                     TGPFLFPDAPVALPLVGGQQGYGLQIFHSASGAHVVLVQTGSNGIDGNGITGYALVAQ
                     "
     gene            complement(567..1064)
                     /locus_tag="VIN75_06700"
     CDS             complement(567..1064)
                     /locus_tag="VIN75_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973889.1"
                     /translation="MTRLALAPVGAGDASALHAMLLALAEGDGVAEVRTTVAALGAAL
                     SGPVPLVVAHFIISDGDVAGFTMHSWKWGTFTGVADLYLNALYVRPEHRRHGAARWAM
                     AELARIAVAAGCTRIEWLTVGARTGTAAFYDAIGSTPASHMTPRRLHGQALRALVNEQ
                     RGQIA"
     gene            complement(1075..>2006)
                     /locus_tag="VIN75_06705"
     CDS             complement(1075..>2006)
                     /locus_tag="VIN75_06705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY8973890.1"
                     /translation="IEVVARACKHIAIAVNKGALGDVMGSADSENVQGEIQKKLDIIS
                     NEVLIEANEWGGHLAAMASEEMDTIHVVPNRYPQGEYLLLFDPLDGSSNIDVNVSIGT
                     IFSVLRKVGHHHGVAEDDFLQAGKNQAAAGYCIYGPQTMLVLTVGDGVAMFTLDREMG
                     SWVLTAENVRIPEDTKEFAINMSNQRHWAPPVTRYIDECLQGKDGPRGKDFNMRWVAS
                     MVADVHRILSRGGVFMYPWDQREPEKAGKLRLLYEANPMSFLVEQAGGASTNGRQRIM
                     DLVPGKLHERVAVFLGSKNEVERATQYHLEAQP"
BASE COUNT          279 a          713 c          689 g          325 t
ORIGIN      
        1 cacgaatgtg tacgccggcc ggctcgacgg cgtggcgtcg atcaccagcc tcagccactc
       61 ggcgcagaac caggaagccg tgctgctgga aggcgccccc ggcctggcgc tgccgaccag
      121 ctacgaacgc ttcacgggcc cgttcctgtt cccggacgcc ccggtggcgc tgcccctcgt
      181 gggcggccag cagggttacg gcctgcagat cttccacagc gcctccggcg cccatgtggt
      241 gctggtgcag actggcagca acggcatcga cggcaacggc atcaccggct acgcgctggt
      301 agcgcaatag gcggcctgcg ccgctccctc gagaacggac gccgcgcggc gtccgtgctt
      361 ccttcttgat gcgtcagcgg ggtgcgcgca gagggcgagc gcgatctcca ggccgtcggc
      421 gacgtccttg tcgtccttga acgggtcggt gccggacgct cgacgactgc gccaggttcc
      481 agatcagcgg cgcgacggcg ccgccctgcg cggcgaagtc gcgcgcaccg ctgccgtcga
      541 tgccggcgag ttcgaccgac gtcttctcat gcgatctgcc cccgttgttc gttcacgagc
      601 gcgcgcaggg cctgcccgtg caggcgccgc ggcgtcatgt ggctggcagg cgtcgagccg
      661 atcgcgtcgt agaacgcggc cgtgcccgtc ctggcgccca ccgtgagcca ctcgatgcgc
      721 gtgcagcccg cggcgacggc gatgcgcgcc agctcggcca ttgcccatcg tgcggcgcca
      781 tgccggcggt gttccggccg cacgtacagc gcgttcaggt acagatcggc cacgccggtg
      841 aacgtgcccc acttccacga atgcatcgtg aagcctgcga cgtcgccgtc gctgatgatg
      901 aagtgggcga cgaccagcgg cacggggccc gacagcgcgg cccccagcgc ggccacggtc
      961 gtgcgcacct cggcgactcc gtcgccctcg gccagcgcca gcaacatcgc gtgcagcgcc
     1021 gaggcgtcgc ccgcgccgac gggcgcgagc gcgagacgcg tcaccgcgcc acgctcacgg
     1081 ctgcgcctcg aggtggtact gcgtcgcgcg ctcgacctcg ttcttcgagc cgaggaacac
     1141 ggccacgcgc tcgtgcagct tgcccggcac caggtccatg atgcgctggc ggccgttggt
     1201 cgaggcgccg ccggcctgct cgaccaggaa gctcatcggg ttggcctcgt acagcaggcg
     1261 cagcttgccg gccttctcgg gctcgcgctg gtcccacggg tacatgaaga cgccgccgcg
     1321 gctgaggatg cggtgcacgt cggccaccat cgaggcgacc cagcgcatgt tgaagtcctt
     1381 gccgcgcggg ccgtccttgc cctgcaggca ctcgtcgatg tagcgcgtga ccggcggcgc
     1441 ccagtggcgc tggttggaca tgttgatcgc gaattccttg gtgtcctccg ggatgcgcac
     1501 gttctcggcg gtgagcaccc acgagcccat ctcgcggtcg agggtgaaca tcgccacgcc
     1561 gtcgcccacg gtcagcacca gcatggtctg cgggccgtag atgcagtagc ccgcggccgc
     1621 ctggttcttg ccggcctgca ggaagtcgtc ctcggccacg ccgtggtggt ggccgacctt
     1681 gcgcagcacc gagaagatgg tgccgatcga gacgttgacg tcgatgttgg acgagccgtc
     1741 cagcgggtcg aacagcagca ggtactcgcc ctgcggatag cggttgggca cgacgtggat
     1801 cgtgtccatc tcttcggacg ccatggccgc caggtggccg ccccattcgt tggcctcgat
     1861 cagcacctcg ttggagatga tgtccagctt cttctggatc tcgccctgca cgttctcgga
     1921 atcggccgag cccatgacgt cgccgagcgc gcccttgttg acggcgatcg cgatgtgctt
     1981 gcaggcgcgc gcgacgacct cgatca
//
LOCUS       DATCRO010000248         6943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_272846, whole genome shotgun sequence.
ACCESSION   DATCRO010000248 DATCRO010000000
VERSION     DATCRO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272846"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3153
                     /locus_tag="VIN75_06710"
     CDS             <1..3153
                     /locus_tag="VIN75_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8973891.1"
                     /translation="LALLLVGIAVFPLLPIAPLPQVDFPTIQVSANLPGASPETMASN
                     VAQPLERNFSLIAGLTQMTSTNSIGSTQITLQFDLNRSIDAAALDVQAAINASTGQLP
                     ANLPSPPTFRKVNPADSPIMLLTVQSRTLPLIEVNDFADNILAQQISQITGVGQVRIG
                     GAQKPAIRIQVDPIKLAAMDMTLEDVRTIIGTMSVNQPKGTIDGPTQSFTVYTNDQLL
                     AAKPWNDIILAYRHGAPIRVRDIGVAVDAPEDNKVHAWAYAGAGSPDSTIQNGRGMIL
                     VITKQPGANVIETVDAIKKALPRLEASIPPTVEVNTLVDRTQTIRASVSDVEHTLMLT
                     IALVVMVIFVFLRNVAATLIPSVTVPLALMGTVAVMYLLDYSLDNLSLMALTIAVGFV
                     VDDAIVMLENIYRHVEEGMSPMEAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIVG
                     RLFREFAVTVTLTIGVSVLISLTLTPMLCSRFLKNEHATRHGRIYQLFERGFDAMLAG
                     YKRGLDVVFKHQFATLLVFIATVAATVVLFLVIPKGFFPQQDTGFIFGNAQSSQDSSF
                     ESMNRRMVEFADIVRQDPDVSGIGMFGNSGQFNTGNFFIALKPKEDGRTASADEIIAR
                     LRPRLAKVGGASLLMQAGQDINVGGRLARTQYQYTITDANLDELNEWAPKLQARFAQL
                     PQLTDVASDLQNAATTASLTIDRDRAASFGITPAAIDSTIYDAIGQRQVSQFFTQINS
                     YHVVLEVTPAMQADPSLFDKLYVMSPLTGQAVPLSTFVKVDTTRTAYLSISHQSQFPA
                     VTLSFNLAPGVALGQAVDAINKAQAEMGLPQALSGSFQGTAQAFRDSLATQPYLIAAA
                     LIAVYIVLGLLYESFIHPLTILSTLPSAGVGALLILMAGGYDLTVIALIGIILLIGIV
                     KKNGIMMVDFALTAERDQGMKPEEAIYRACLLRFRPIMMTTMCALLSGLPLMLGHGQG
                     SELRRPLGFAMVGGLLLSQVLTLFTTPIVYLYLDRAHYWYEKKHEARRKRKAARRLAA
                     DGTDPDRSLPVIADDGPLER"
     gene            3170..3871
                     /locus_tag="VIN75_06715"
     CDS             3170..3871
                     /locus_tag="VIN75_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY8973892.1"
                     /translation="MKLLIVEDEVKTAEYLQKGLAEQGCAVDVAHNGIDGQHLALVHD
                     YDVIVLDAMLPGIDGFTLLRSLRAVKQTPVIMLTARDSIEDRVRGLHDGADDYLVKPF
                     SFLELLARLQALQRRGRAQEPTQLKIANLQIDLIARKAWRGSERIDLSAKEFALLAVL
                     ARRQGEILSKTAIAELVWDMNFDSNTNVVEVAIKRLRAKVDNAFTPKLLHTIRGMGYV
                     MEARDDGAAPAEPGP"
     gene            3868..5259
                     /locus_tag="VIN75_06720"
     CDS             3868..5259
                     /locus_tag="VIN75_06720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY8973893.1"
                     /translation="MTRSITARLVLMFALGALATLTLIGAALYGVLERELTRHQNDEL
                     NTNLQNMRYSLERFGDLEHWARLQVKMDTLTPADGSVRFWVLSDDPRFLYGKGLDEME
                     RVNQGEAGRGMLTLPITGRTYRTLSMRIAPFQDRPAVRLIVGVDTAPFEHTLRAFLVA
                     LTLLTLGAVALVMGVGWWIARIGLRPLQRLSEQARALRPKTLSQRLHLERLPVELEDL
                     TTAFNGALGRLEEAYQQLEGFNADVAHELRTPLANLIGGTQVALSRPRSAGEFQDTLQ
                     SNLEELERLRSIVNDMLFLARADQGEAATGLTAADVAHEVEMTIEFFEPLLDETGTTV
                     SIEGELRAQATMNIALFRRALSNLLQNAIEHSAAGAKLVVTISQRAGAIWIAVANPGK
                     TIAATHLPRLFDRFYRVDSSRHDAGETHGHGLGLAIVKAVASMHGGATLATSEAGLTT
                     IAFSIADRAPAAA"
     gene            complement(5323..>6943)
                     /gene="ggt"
                     /locus_tag="VIN75_06725"
     CDS             complement(5323..>6943)
                     /gene="ggt"
                     /locus_tag="VIN75_06725"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY8973894.1"
                     /translation="IHPVHSLHGMVSSESALASDVGVEVLRKGGNAVDAAVATGFALA
                     VTMPNSGNIGGGGFMILHDGKTGKDTAIDFREMAPAAASRNMYLDASGNVIQNKSLFT
                     QAAIGVPGTVAGLTYALEHYGTMKRADLVAPAVKLANNGFPVGETLAGLFVAEHDHLV
                     KWEGTRKVFEHHGKLPVTGELLRNPDLGKSLALIGRDGAKAFYDGPIGAAIVKEAQAH
                     DGLITADDLKNYKVVERTPVRGNYRGYEIVSMPPPSSGGAHVVQILNILSNFPLKDWG
                     PNSARTIHVMAEAEKRAYADRSEYLGDPDFVKVPLKGITSPAYAKELASQIDLEHATP
                     SAQIKPGKPQPYESDQTTHFVVADSHGSVVSVTYTLNTNFGSGIVASGTGILLNNEMD
                     DFSAKPGVPNAYGLVGGDANAVEARKRPLSSMSPTIVLKDGKPWLVTGSPGGARIITT
                     TLQVLVNCIDFGMNPAEAGSAVRVHHQWLPDELRIERGLSPDTVAILRSMGHKVVERQ
                     SMGDTQTIQILPTGFYGYADQRDPNGAARGY"
BASE COUNT         1073 a         2430 c         2339 g         1101 t
ORIGIN      
        1 ctggcgctgc tgctggtggg catcgcggtg ttcccgctgc tgcccatcgc gccgctgccg
       61 caggtcgact tccccaccat ccaggtctcg gccaacctcc cgggcgccag cccggagacg
      121 atggcgtcga acgtggcgca gccgctggag cgcaacttct cgctgatcgc gggcctgacg
      181 cagatgacgt cgaccaattc catcggctcg acccagatca cgttgcagtt cgacctcaac
      241 cgctcgatcg acgccgccgc gctcgacgtg caggccgcca tcaacgcgtc caccgggcag
      301 ctgccggcca acctgccgag cccgccgacg ttccgcaagg tcaacccggc ggactcgccg
      361 atcatgctgc tgacggtgca atcgaggacg ctgccgctga tcgaggtcaa cgacttcgcc
      421 gacaacatcc tcgcgcagca gatctcgcag atcacgggcg tcggccaggt gcgcatcggc
      481 ggcgcgcaga agccggcgat ccgcatccag gtcgacccga tcaagctcgc cgcgatggac
      541 atgaccctgg aggacgtgcg caccatcatc ggcacgatgt ccgtcaacca gcccaagggc
      601 acgatcgacg gccccacgca gagcttcacc gtctacacca acgaccagct gctggcggcc
      661 aagccgtgga acgacatcat cctggcgtac cgccacggcg cgccgatccg ggtgcgcgac
      721 atcggggtgg ccgtggacgc gcccgaggac aacaaggtgc acgcctgggc ctatgccggc
      781 gcgggcagcc cggacagcac gatccagaac ggccgcggca tgatcctggt gatcaccaag
      841 cagcccggcg ccaacgtcat cgagacggtg gacgccatca agaaggcgct gccgcggctg
      901 gaggcgtcga tcccgccgac cgtcgaggtc aacacgctgg tcgaccgcac gcagacgatc
      961 cgcgcgtcgg tcagcgacgt cgagcacacg ctgatgctga cgatcgcgct ggtggtgatg
     1021 gtgatcttcg tgttcctgcg caacgtcgcc gccacgctga ttcccagcgt caccgtgccg
     1081 ctcgcgctga tgggcacggt cgcggtgatg tacctgctcg actacagcct ggacaacctg
     1141 tcgctcatgg cgttgacgat cgcggtcggg ttcgtggtcg acgacgcgat cgtgatgctg
     1201 gagaacatct accgccatgt cgaggagggc atgagcccga tggaggccgc gctcaagggc
     1261 gcgggcgaga tcggcttcac gatcatctcg atctcggtgt ccctggtggc ggtgttcatc
     1321 cccttgctgc tgatgggcgg catcgtgggc cggctgttcc gcgagttcgc ggtgacggtg
     1381 acgctgacga tcggcgtgtc ggtgctgatc tcgctgacgc tcacgccgat gctgtgctcg
     1441 cgcttcctca agaacgagca cgccacgcgc cacggccgca tctaccaact gttcgaacgc
     1501 ggcttcgacg cgatgctggc cggctacaag cgcgggctcg acgtcgtgtt caagcaccag
     1561 ttcgccacgc tcctggtgtt catcgcgacc gtggccgcca ccgtggtgct gttcctcgtc
     1621 atccccaagg gcttcttccc gcaacaggac accggcttca tcttcggcaa cgcgcagtcc
     1681 tcgcaggact cttcgttcga gtcgatgaac cgccgcatgg tggagttcgc cgacatcgtg
     1741 cgccaggacc cggacgtgtc gggcatcggc atgttcggca actcggggca gttcaacacg
     1801 ggcaacttct tcatcgcgct caagcccaag gaggacgggc gcaccgccag cgccgacgag
     1861 atcatcgcgc gcctgcggcc caggctggcc aaggtgggcg gcgcctcgct gctgatgcag
     1921 gcgggccagg acatcaacgt cggcggccgc ctggcgcgca cccagtacca gtacaccatc
     1981 accgacgcca acctcgacga gctcaacgaa tgggcgccca agttgcaggc ccgcttcgcg
     2041 cagctgccgc agctgaccga cgtcgcctcc gacctgcaga acgccgccac caccgcgagc
     2101 ctgacgatcg atcgcgaccg cgcggccagc ttcggcatca cgccggcggc gatcgactcg
     2161 acgatctacg acgcgatcgg ccagcgccag gtcagccagt tcttcacgca gatcaacagc
     2221 taccacgtcg tgctggaggt gacgccggcg atgcaggccg acccgtcgct gttcgacaag
     2281 ctctacgtca tgtcgccgtt gaccggccag gcggtgccgc tgtccacgtt cgtgaaggtc
     2341 gacacgacca ggacggccta cctgtcgatc agccaccaga gccagttccc ggcggtgacg
     2401 ctgtcgttca acctggcgcc cggcgtggcg ctgggccagg ccgtcgacgc gatcaacaag
     2461 gcgcaggccg agatgggcct gccgcaggcg ctgtcgggct cgttccaggg cacggcgcag
     2521 gccttccgcg attcgctggc cacgcagccc tacctgatcg cggcggcgct gatcgccgtc
     2581 tacatcgtgc tgggcctgct gtacgagagc ttcatccacc cgctgaccat cctgtcgacg
     2641 ctgccgtcgg cgggcgtggg ggcgctgctg atcctgatgg cgggcggcta cgacctgacc
     2701 gtcatcgcgc tgatcggcat catcctgctg atcggcatcg tcaagaagaa cggcatcatg
     2761 atggtggact tcgcgctcac cgccgaacgc gaccagggca tgaagcccga ggaggcgatc
     2821 taccgcgcct gcctgctgcg cttccgcccg atcatgatga cgacgatgtg cgccctgctg
     2881 tcgggcctgc cgctgatgct gggccacggc cagggctccg agctgcgccg gcccctgggc
     2941 ttcgcgatgg tgggcggcct gctgctgtcg caggtgctca cgctgttcac cacgcccatc
     3001 gtctacctgt acctggatcg cgcgcactac tggtacgaga agaagcacga ggcgcgccgg
     3061 aagcgcaagg cggccaggcg gctcgccgcc gacggcaccg accccgaccg cagcctgccc
     3121 gtgatcgccg atgatggccc tctcgaacgc tgacatccgg ttgaccgaca tgaagctgct
     3181 gatcgtcgag gacgaagtca agaccgcgga gtacctgcag aagggcctcg cggaacaggg
     3241 ctgcgccgtg gacgtcgcgc acaacggcat cgacgggcag cacctggcgc tggtgcacga
     3301 ctacgacgtc atcgtcctcg acgccatgct gccgggcatc gacggcttca cgctgctgcg
     3361 ctcgctgcgg gccgtcaagc agacgcccgt catcatgctc acggcgcgcg actccatcga
     3421 ggaccgcgtg cgcggcctgc atgacggtgc ggacgactac ctcgtcaagc cgttctcgtt
     3481 cctcgagctg ctcgcgcgcc tgcaggcgct gcagcggcgc gggcgcgcgc aggagcccac
     3541 gcagctcaag atcgccaacc tgcagatcga cctgatcgca cgcaaggcct ggcgcggcag
     3601 cgagcgcatc gatctcagcg ccaaggagtt cgcgctgctg gcggtgctcg cgcggcgcca
     3661 gggcgagatc ctgtccaaga ccgcgatcgc cgagctcgtg tgggacatga acttcgactc
     3721 caacaccaac gtcgtcgagg tcgcgatcaa gcggctgcgc gccaaggtcg acaacgcgtt
     3781 cacgcccaag ctgctgcaca cgatccgcgg catgggctac gtgatggagg cgcgcgacga
     3841 cggcgccgcg cccgcggagc cgggcccatg acgcgctcga tcaccgcccg gctggtgctg
     3901 atgttcgccc tcggcgcgct ggccacgctc acgctgatcg gcgccgcgct gtacggcgtg
     3961 ctcgagcgcg aactcacgcg ccaccagaac gacgagctca acaccaacct gcagaacatg
     4021 cgctattccc tcgagcgctt cggcgacctc gagcactggg cccgcctgca ggtcaagatg
     4081 gacaccctca cgccggccga cggcagcgtg cgcttctggg tgctcagcga cgacccgcgc
     4141 ttcctctacg gcaaggggct cgacgagatg gagcgcgtca accagggcga ggccggccgc
     4201 ggcatgctga cgctgccgat caccgggcgc acctaccgga cgttgtcgat gcgcatcgcg
     4261 ccattccagg accgcccggc cgtgcgcctg atcgtgggcg tcgacacggc gccgttcgag
     4321 cacacactgc gcgccttcct cgtcgcgctg acgctgctga cgctcggcgc ggtggcgctc
     4381 gtcatgggcg tgggctggtg gatcgcccgc atcggcctgc gtccgctgca gcggctgtcg
     4441 gagcaggcgc gcgcgctgcg cccgaagacg ctgtcgcagc gcctgcacct ggagcggctg
     4501 ccggtcgaac tggaggatct caccacggcc ttcaacggcg cgctcgggcg gctcgaggag
     4561 gcctaccagc agctcgaggg cttcaacgcc gacgtcgccc acgagctgcg cacgccgctg
     4621 gccaacctga tcggcggcac gcaggtggcg ctgtcgcggc cgcgcagtgc gggcgagttc
     4681 caggacacgc tgcagtccaa cctcgaggag ctggaacgcc tgcgctccat cgtcaacgac
     4741 atgctgttcc tcgcgcgcgc cgaccagggg gaggccgcca ccggcctgac cgcggccgac
     4801 gtcgcgcacg aggtcgagat gaccatcgag ttcttcgagc cgctgctcga cgagaccggc
     4861 accaccgtct cgatcgaggg cgagctgcgc gcccaggcca cgatgaacat cgcgctgttc
     4921 cggcgcgcgc tgtccaacct gctgcagaac gcgatcgagc attcggcggc gggcgcgaag
     4981 ctggtggtga cgatctcgca gcgcgccggc gccatctgga tcgccgtcgc caacccgggc
     5041 aagacgatcg ccgccacgca cctgccgcgc ctgttcgacc gcttctaccg cgtcgactcg
     5101 tcgcgtcacg acgcgggcga gacccacggc cacggcctgg gcctggccat cgtcaaggcg
     5161 gtggcgtcga tgcacggtgg ggcgacgctg gccaccagcg aggcggggct gacgacgatc
     5221 gcgttcagca ttgccgatcg cgcacccgcg gcggcctgag gccttgcggc tctggtcgat
     5281 ccggttggcg ccacggccat cgcgcggtcc gcgcccgcgg cctcaatacc cgcgcgccgc
     5341 cccgttcgga tcgcgctggt cggcatagcc gtagaacccc gtcggcagga tctggatggt
     5401 ctgcgtgtcg cccatcgact ggcgctcgac caccttgtgg cccatcgagc gcaggatggc
     5461 caccgtgtcc gggctcagcc cgcgctcgat gcgcagctcg tcgggcagcc actggtggtg
     5521 cacgcgcacg gcggagccgg cctcggccgg gttcatgccg aagtcgatgc agttgaccag
     5581 cacctgcagc gtcgtcgtga tgatgcgcgc gccgccgggc gagccggtga ccagccaggg
     5641 cttgccgtcc ttgagcacga tggtgggcga catcgagctc agcggccgct tcctcgcctc
     5701 gaccgcgttg gcgtcgccgc ccaccaggcc gtaggcattg ggcacgccgg gcttggcgga
     5761 gaagtcgtcc atctcgttgt tgagcaggat gcccgtgccc gaggcgacga tgcccgagcc
     5821 gaagttcgtg ttgagcgtgt acgtgacgct gaccacgctg ccgtggctgt cggcgacgac
     5881 gaagtgcgtg gtctggtcgc tttcgtaggg ctgcggcttg cccggcttga tctgcgcgga
     5941 cggcgtggcg tgctccaggt cgatctggct ggcgagctcc ttggcgtagg ccggtgacgt
     6001 gatgcccttg agcggcacct tcacgaagtc cgggtcgccg aggtactcgg aacggtcggc
     6061 gtaggcgcgc ttttcggcct cggccatgac atggatcgtc ctggcgctgt tggggcccca
     6121 gtccttgagc ggaaagttcg acaggatgtt caggatctgc acgacgtgcg cgccgccgga
     6181 actgggcggc ggcatggaga cgatctcgta gccgcggtag ttgccgcgca cgggcgtgcg
     6241 ctcgacgacc ttgtagttct tcaggtcgtc ggccgtgatg aggccgtcgt gcgcctgggc
     6301 ctccttgacg atggccgcgc cgatcgggcc gtcgtagaac gccttggcgc cgtcgcggcc
     6361 gatcagcgcg agactcttgc cgaggtcggg attgcgcagc agctcgccgg tgaccggcag
     6421 cttgccgtgg tgctcgaaca ccttgcgcgt gccctcccac ttgaccaggt ggtcgtgctc
     6481 ggcgacgaac aggccggcca gcgtctcgcc gaccgggaag ccgttgttgg ccagcttgac
     6541 ggccggcgcg acgaggtcgg cgcgcttcat cgtgccgtag tgctccagcg cgtacgtgag
     6601 gccggccacg gtgccgggca cgccgatggc ggcctgcgtg aacagcgact tgttctggat
     6661 gacgttgccg ctcgcgtcga ggtacatgtt gcgcgacgcg gccgcggggg ccatctcgcg
     6721 gaagtcgatg gcggtgtcct tgcccgtctt gccgtcgtgc aggatcatga agccgccgcc
     6781 gccgatgttg ccggaattgg gcatcgtcac ggccagcgcg aaaccggtgg ccaccgcggc
     6841 gtccacggcg ttgccgccct ttcgcagcac ctccacgccc acgtcggagg cgagcgcgct
     6901 ctcggagctg accatgccgt gcagcgagtg caccggatgg atg
//
LOCUS       DATCRO010000249         9456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_655479, whole genome shotgun sequence.
ACCESSION   DATCRO010000249 DATCRO010000000
VERSION     DATCRO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655479"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VIN75_06730"
     CDS             <1..351
                     /locus_tag="VIN75_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEY8973895.1"
                     /translation="DLMRHLPDAPPGTMDFLLVRILQHLQAQGYRHFGLGMSPMAGMA
                     RRSGAPKWQRVARLVYEHGNGFYNFRGLRSFKDKFSPVWEARFLAAPRGVAAAFILAD
                     VTTLISGSKVPQKT"
     gene            348..1694
                     /locus_tag="VIN75_06735"
     CDS             348..1694
                     /locus_tag="VIN75_06735"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEY8973896.1"
                     /translation="MTDALVPLARRMRRPLAALVATACMAAPTPSLAAQPASSPESAA
                     LSHGLFSQLRVHRPAGATRHFVLLLTHGTAPDAREQAMLRTMLAADAMVVTVPFAPFY
                     RRLAAQDGRCTYGPGAFENLARYLQAYEQVPGYLLPMVAGAGSTASYAHTLVSQAPAG
                     TFAAGLGLAPQALALKPPPCAPNAAAAVPRDAWGVHAATATATPWIAVPADARPAAGT
                     APDSTAEFARAFRQVGGQQAALGAPPAELGNLPIVEVPAARPGSRFAVLLSGDGGWAG
                     IDKSLGAALAARGVSVAGFDSLRYFWGVRTPDELAADLDRVIRYYAAHWGRSEVLLVG
                     YSQGADVLPFALNRLPPATRASVRLTALLGPGQKASFEFHLSNWIGPGGDRPIAPEAL
                     KLDAADTLCIYGLDEKDSLCPELSPVHARAIPLKGGHHFGGDYDALAGLILDAPPR"
     gene            complement(1788..1880)
                     /locus_tag="VIN75_06740"
     tRNA            complement(1788..1880)
                     /locus_tag="VIN75_06740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Ser,seq:gct)
     gene            complement(1920..3197)
                     /locus_tag="VIN75_06745"
     CDS             complement(1920..3197)
                     /locus_tag="VIN75_06745"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8973897.1"
                     /translation="MALIVHKYGGTSMGSTERIRNVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLGLAKELFPGVPTPASDRELDMIASTGEQVSVGLLSLALQAEGLEAVSYTGW
                     QVAVKTDSSYTKARIESIDDDRVRADLAAGKVVVITGFQGIDEAGHITTLGRGGSDTS
                     AVAIAAAMKAAECLIYTDVDGVYTTDPRVVPEARRLQTISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPLRVLSSFTDWNIDINEEARSGTLITFEEDEKMEQAVVSGIAFNRDEA
                     KVTVMGVPDKPGIAYQILGDVAEANIDVDVIIQNVSHDGKTDFSFTVHRNDYARTMDL
                     LKNKVAPATGAASVVGDPKICKVSIVGIGMRSHVGVASKMFRTLSEEGINIMMITTSE
                     IKTSVVIDEKYMELAVRALHKAFDLEEPSASAS"
     gene            complement(3403..4710)
                     /gene="tilS"
                     /locus_tag="VIN75_06750"
     CDS             complement(3403..4710)
                     /gene="tilS"
                     /locus_tag="VIN75_06750"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY8973898.1"
                     /translation="MTDRPVVAVACSGGRDSVALLHAARRAGDALGTSVVALHVHHGL
                     MADADRWAAELAQLCGRWGVRLLESRLPGAPQAGESVEAWARRERYAALARMAREAGA
                     TIVLLGHHRHDQAETVLLQALRGAGPAGLAAMPRVVERDGLTWARPWLARTGAQIDAY
                     VAPLGVAVAADPSNADPRFARSRLRHGVMPALRRAFPDADIALAAVARRAGEAKAVLD
                     EVAAEDLQRLGAGDVLPLPAWRALSPARRANALRTWLGRMLAGPVPQTLVDRLLAEAT
                     GEATRRWPIDGGHVLRAWRGQLAVVAPSRARASVTPAGATGRAGESLSLLRCDAYALP
                     GWDGALEVFATAERGVAPHRLAQVEARVRAGGERFALNPAGGARSLKKQWQSQGIPAE
                     GRDGPLVYDATGALLFVPGLGIDARAWAPEGAPQWGLRWRPPA"
     gene            complement(4721..5692)
                     /locus_tag="VIN75_06755"
     CDS             complement(4721..5692)
                     /locus_tag="VIN75_06755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY8973899.1"
                     /translation="MTKRHFLEFEQPIAELESKIEELRYVQNESAVDISEEIDRLDKK
                     SLQLTKDIYSSLEPWQVYQIARHPQRPQTLDYTAEVFTEFEELHGDRSFADDAAIVGG
                     LARFNGQACMVVGHQRGYDTKDRQLRNFGMPRPEGYRKAIRLMKTAEKFGLPVFTFID
                     TMGAYPGIGAEERGQSEAIGASIFEMAQLEVPIISTIIGEGGSGGALAIGVCDQMLML
                     QFSAYSVISPEGCASILWKTSERAAEAAEALGITAHRLKALGLIDKIVNEPVGGAHRD
                     PKAMASSLKRALADGLRQVADLKPKDLLARRQERLLAYGRFADTTDR"
     gene            complement(5719..6390)
                     /locus_tag="VIN75_06760"
     CDS             complement(5719..6390)
                     /locus_tag="VIN75_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY8973900.1"
                     /translation="MASPAAAQAASDAARDVPAYWDDACRHLVKRDRVMRKLIPQFGD
                     ARLQSNGDAFTTLARSIVGQQVSTKSAQGVWRKLVGAVDGPDVELLQPAAVLGLAAEA
                     LRGVGLSARKVEYLRDLAHNFSSGAVHVGLWHGMDDEAIIEELVAIRGIGRWTAEMFL
                     IFHLLRPNVLPLDDPNLIRGISENYFSGEPVSRAEARELGEGWAPYRSVATWYLWRSL
                     DPVKG"
     gene            complement(6392..7807)
                     /gene="cysS"
                     /locus_tag="VIN75_06765"
     CDS             complement(6392..7807)
                     /gene="cysS"
                     /locus_tag="VIN75_06765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8973901.1"
                     /translation="MTLRLFNTLTGQAEEFVPIVPGRVRMYVCGMTIYDLCHMGHARM
                     MMAFDVVYRWLQVLGYEVTYVRNITDIEDKIIKRAVERGITIRQLTDEMIAAMREDIG
                     AIGIEPPTHEPRATEYVPQMLAMIDVLRDKGLAYQGDNGDVNYAVRKFAGYGKLSGKS
                     LDDLRAGERVAVADGKTDPLDFVLWKSAKPSEPDDAKWDSAYGPGRPGWHIECSAMSC
                     AVLGERFDIHGGGMDLQFPHHENEIAQSEGALGHPFVNYWLHNGFLNVDNEKMSKSLG
                     NFFTIRDVLKKFDGETLRFFMLRTHYRSPFNFSDAHLDDARSALRRLYTALASVGLHP
                     EHAAAGATEQPVDAAAIDWTDPRAAAFRDAMNDDFNTPGALAVLFEMVTDLNRTKSVE
                     TAALLRALGATLGVLQQEPTAFLQAGAALTDAEIQARIEARAQAKKNKDFAESDRIRQ
                     ELADLGITLKDGPEGTTWVKA"
     gene            8005..8574
                     /locus_tag="VIN75_06770"
     CDS             8005..8574
                     /locus_tag="VIN75_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8973902.1"
                     /translation="MSIPQQTRWAALALAGLSAFAAPAFAGDKVHLDTTLGEIVVELD
                     ADKAPKTVANFEQYVKEGHYNGTVFHRVIPNFMIQGGGMVSTFAGLKEKPTHKPIVNE
                     SHNGLSNTTGTIAMARTGDPNSATAQFFINVKDNPFLDQGDGYAVFGHVVSGMDVVEK
                     IKMVETTTKGPFENVPVTPVIIKKATLEK"
     gene            8576..9079
                     /locus_tag="VIN75_06775"
     CDS             8576..9079
                     /locus_tag="VIN75_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8973903.1"
                     /translation="MTKYVKMTTTQGDLRIELDDAKAPISVANFLSYVNKGFYDGTIF
                     HRVIRNFMIQGGGFESGMKQKDTDAQIQNEAKNGLKNDKYTLAMARTSAPHSATAQFF
                     INTTNNGFLNNDQAQDGWGYAVFGKVVQGQEVVDAIGGVKTGRKGFHDDVPLEDVKIV
                     KAEEVQA"
     gene            9082..>9456
                     /locus_tag="VIN75_06780"
     CDS             9082..>9456
                     /locus_tag="VIN75_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8973904.1"
                     /translation="MAATFDAELKLGDDIRVVDFISDIHLSETMPATADAWEAYMART
                     QAGAVFLLGDIFEVWVGDDARTRPFEARCTAAIAATAARVPVWLQHGNRDFLIGAEFA
                     QATGIRLLADPASVTAFGRTVLL"
BASE COUNT         1421 a         3347 c         3223 g         1465 t
ORIGIN      
        1 gacctgatgc gccacctgcc ggacgccccg ccgggaacga tggacttcct cctggtgcgc
       61 atcctgcagc acttgcaggc gcagggctac cgccacttcg gcctgggcat gtcgccgatg
      121 gccggcatgg cgcgacgcag cggcgccccg aaatggcagc gcgtcgcccg cctggtgtac
      181 gagcacggca acgggttcta caacttccgg ggcctgcgca gcttcaagga caagttctca
      241 cctgtctggg aagcccgctt cctggcggcg ccacgcggcg tcgccgccgc cttcatcctg
      301 gctgacgtga ccacgctcat cagcggatcc aaggttccgc aaaagacatg accgacgccc
      361 tcgtgcccct cgcgcgccgc atgcggcggc cgctcgccgc gctcgtcgcg acggcctgca
      421 tggccgcgcc gacgccctcg ctcgccgcgc agccggcttc gtcgcccgaa tccgccgcgt
      481 tgtcgcacgg cctgttcagc cagctgcgcg tccatcgccc ggccggcgcg acgcgtcatt
      541 tcgtgctcct gctgacgcac ggcacggcgc cggatgcccg cgagcaggcg atgctgcgca
      601 cgatgctggc cgccgacgcc atggtcgtga ccgtgccctt cgcgccgttc taccgccggc
      661 tggcggcgca ggacggccgc tgcacctacg ggcccggcgc gttcgagaac ctcgcgcgct
      721 acctgcaggc ctacgagcag gtgcccggct acctgctgcc gatggtcgcc ggcgcggggt
      781 cgacagcgtc ctacgcgcac acgctggtct cgcaggcccc ggccggcacg ttcgccgccg
      841 gcctggggct ggcgccgcag gcgctggcgt tgaagccgcc gccgtgcgcg ccgaacgccg
      901 cggccgccgt gccgcgcgac gcctggggcg tccatgccgc cacggcgacc gcgacgccct
      961 ggatcgcggt gccggcggac gcacggccgg ccgccggcac cgcgccggat tccacggcag
     1021 aattcgctcg cgcgttccgc caggtcggcg gtcaacaggc cgcgctgggc gcgccgccgg
     1081 ccgaactcgg caacctgccc atcgtcgagg tccccgcggc gcggcccggc agccgcttcg
     1141 cggtgctgct ctcgggcgac ggcggttggg ccggcatcga caagagcctg ggcgccgccc
     1201 tcgccgcacg gggcgtatcg gtcgccggct tcgattccct gcgctacttc tggggcgtgc
     1261 gcacgccgga cgaactggcc gccgacctcg accgcgtgat ccgctactac gccgcgcact
     1321 ggggccgcag cgaggtgctg ctggtggggt actcgcaggg cgccgacgtg ctgccgttcg
     1381 ccctcaaccg gctgccgccc gccacgcgcg ccagcgtccg gttgaccgcc ctgctcggcc
     1441 cgggccagaa ggcctcgttc gaattccacc tgtccaactg gatcggcccc ggcggcgacc
     1501 ggccgatcgc gcccgaggcc ttaaagctcg acgccgcgga cacgctgtgc atctatggcc
     1561 tcgacgagaa ggactcgctg tgcccggagc tctcgcccgt ccacgcacgc gcgatcccgc
     1621 tcaagggcgg acaccatttc ggcggcgact acgacgcact ggccggactc atcctcgacg
     1681 cgccgccgcg ttgatgcacg cggacctgcg ccgtcgcgaa tgccggcggc ccggatgcgc
     1741 catgagaaaa gccccagaag atttctcctc tggggcttcg cgtcggttgg cggagttgga
     1801 gggattcgaa ccctcgatgc agctcatcac cgcatgctcc cttagcaggg gagtaccttc
     1861 gaccactcgg ccacaactcc aacctaagct ggcattctag cctggatttt ggccacctat
     1921 caagaagcgg aggccgaggg ttcttccagg tcgaaggcct tgtgcagcgc gcgcacggcg
     1981 agctccatgt acttctcgtc gatgacgacc gacgtcttga tctcgctggt ggtgatcatc
     2041 atgatgttga tgccctcctc gctgagcgtg cggaacatct tggaggcgac gccgacgtgg
     2101 ctgcgcatgc cgatgcccac gatcgagacc ttgcagatct tcggatcgcc gacgacgctg
     2161 gccgcaccgg tggccggggc caccttgttc ttgagcaggt ccatcgtgcg cgcgtagtcg
     2221 ttgcgatgca cggtgaacga gaagtcggtc ttgccgtcgt gcgagacgtt ctggatgatg
     2281 acgtcgacgt cgatgttggc ctcggccacg tcgcccagga tctggtacgc gatgcccggc
     2341 ttgtcgggga cgcccatcac ggtgaccttg gcctcgtcgc ggttgaacgc gatgccggaa
     2401 acaacggcct gttccatctt ctcgtcttcc tcgaaggtga tcagcgtgcc cgagcgggcc
     2461 tcttcattga tgtcgatgtt ccagtcggtg aagctggaca gcacgcgcag cggcacgcgg
     2521 tacttgccgg cgaattccac cgaacggatc tgcagcacct tggagccgag gctggccatc
     2581 tcgagcatct cctcgaacga gatggtctgc aggcggcgcg cctcgggcac cacgcgcggg
     2641 tccgtggtgt agacgccgtc gacgtcggtg tagatcaggc actcggccgc cttcatcgcg
     2701 gcggcgatcg ccaccgccga cgtgtcggag ccgccgcgac ccagcgtcgt gatgtggccg
     2761 gcctcgtcga tgccctggaa gccggtgatg accaccacct tgcccgcggc caggtcggcg
     2821 cgcacgcggt cgtcgtcgat gctctcgatg cgcgccttgg tgtacgacga gtccgtcttc
     2881 acggcgacct gccagccggt gtagctcacg gcctccaggc cttcggcctg cagcgccagc
     2941 gacagcaggc ccaccgacac ctgctccccg gtggaggcga tcatgtcgag ctcccggtcg
     3001 gacgccggcg tgggcacgcc cgggaacagt tccttggcca ggccgaggag gcggttggtc
     3061 tcgccgctca tggccgaggg gacgacgacc atctggtgac cggcgcgcgc ccatttcgcg
     3121 acgcgtttgg cgacgttgcg aatccgctcc gtcgaaccca tcgaggtgcc accgtacttg
     3181 tgaactatca atgccatggt cttcaaacgc ccggttgaac ctggacaggc tcgtctggcc
     3241 tgcccaccca aacctccaag tttatcgcgc gatcaggggt tttcccgggc gactccggtt
     3301 cgtggctggt ggagcggcgc ttctttcggc acgaacacga cttgcctaga caggccgagc
     3361 cttcgcggcc gcggcggacc agggcgcgcc agccttcgcc ggctacgcag gcggccgcca
     3421 gcgcaggccc cactgcggcg ccccttccgg cgcccaggcg cgggcgtcga tgcccaggcc
     3481 cggcacgaac agcagcgcgc ccgtggcgtc gtagaccagc ggcccgtccc ggccctcggc
     3541 cgggatgcct tgcgactgcc attgcttctt caggctgcgc gccccacccg cgggattgag
     3601 cgcgaagcgc tcgccgcccg cgcgaacgcg ggcctcgacc tgcgcgagcc ggtgcggcgc
     3661 gacgccgcgc tcggctgtcg cgaacacctc gagcgccccg tcccaacccg gcaaggcgta
     3721 ggcgtcgcaa cgcagcagcg acaaggactc ccccgcccgc ccggtcgcac ccgcgggcgt
     3781 cacgctcgcg cgagcgcgcg agggcgcgac cacggccagt tggccgcgcc acgcgcgcag
     3841 gacgtggccg ccgtcgatcg gccagcgccg cgtcgcctcg cccgtcgcct cggccagcag
     3901 ccgatcgacc agcgtctgcg gcaccggccc ggccagcatg cggcccagcc aggtgcgcaa
     3961 cgcgttggcg cgccgcgccg gggacagcgc ccgccatgcc ggcagcggca ggacgtcgcc
     4021 ggcccccagg cgctgcagat cctccgcggc gacctcgtcc agcaccgcct tcgcctcgcc
     4081 cgcgcgccgc gcgacggcgg ccagcgcgat gtcggcatcg ggaaacgccc gtcgcagggc
     4141 cggcatcacg ccgtgtcgca agcggctgcg cgcgaagcgc ggatcggcgt tgctgggatc
     4201 ggcggccacg gccacgccca gcggcgcgac gtaggcgtcg atctgcgcgc cggtgcgcgc
     4261 gagccaggga cgggcccacg tcagtccgtc gcgttcgacg acgcgcggca tcgcggccag
     4321 gcccgcgggc ccggcgccgc gcagggcctg cagcagcacg gtttcggcct ggtcatgccg
     4381 gtgatggccc agcagcacga tcgtcgcgcc cgcctcgcgc gccatccggg ccagcgcggc
     4441 atagcgttcc cgccgcgccc aggcctcgac gctctcgcca gcctgcggtg cgccaggcag
     4501 gcggctttcg agcaggcgca cgccccaacg cccgcaaagc tgcgccagtt ccgcggccca
     4561 gcggtcggcg tcggccatga ggccatggtg gacatggagc gcgacgacgc tcgtcccgag
     4621 cgcgtcgccg gcacgccgcg cggcatgcag caaggcgacc gaatcgcgtc cgccgctgca
     4681 ggcaaccgca acgacgggcc gatcggtcat cagctcgtct tcaacggtcc gtcgtgtcgg
     4741 cgaagcggcc gtaggccagc aggcgctcct ggcggcgggc cagcaggtcc ttcggcttca
     4801 ggtcggcgac ctggcgcagg ccatcggcca gcgcgcgctt caggctcgag gccatcgcct
     4861 tcggatcgcg gtgggcccca cccacgggtt cgttgacgat cttgtcgatc aggcccagcg
     4921 ccttcaggcg gtgcgccgtg atgcccagcg cctcggcggc ctcggcggcg cgctccgacg
     4981 tcttccacag gatcgacgcg cagccttccg gcgagatcac cgaataggcc gagaactgca
     5041 gcatcagcat ctggtcgcag acgccgatgg ccagcgcgcc gccggagccg ccttcgccga
     5101 tgatggtgga gatgatgggc acttccagct gcgccatctc gaagatgctg gcgccgatgg
     5161 cctcggactg gccgcgctcc tcggcgccga tgcccgggta cgcgcccatc gtgtcgatga
     5221 acgtgaacac gggcaggccg aacttctcgg ccgtcttcat caggcggatg gccttgcggt
     5281 agccctcggg acgcggcatg ccgaagttgc gcagctggcg atccttggtg tcgtagccgc
     5341 gctggtggcc cacgaccatg caggcctggc cgttgaagcg cgcgaggccg ccgacgatgg
     5401 cggcgtcgtc cgcgaacgag cggtcgccgt gcagttcctc gaactcggtg aacacctcgg
     5461 ccgtgtagtc cagcgtctgc gggcgctgcg ggtggcgcgc gatctggtag acctgccacg
     5521 gctccaggct ggagtagatg tccttggtga gctgcaggct cttcttgtcg aggcggtcga
     5581 tctcttccga gatgtccacg gccgactcgt tctgcacgta gcgcagctcc tcgatcttcg
     5641 attcgagctc ggcgatcggt tgttcgaatt ccaggaagtg gcgtttggtc atcgtttcag
     5701 gtccttttgc gcggcggatc agcccttgac ggggtcaagg ctgcgccaga gataccacgt
     5761 ggcgacggaa cggtacggag cccacccctc gcccagttcg cgggcctcgg cgcgcgagac
     5821 cggctcgccg ctgaagtaat tttcgctgat tccgcggatc aggttcggat cgtccagcgg
     5881 aaggacgttc ggacgcagga gatggaagat caggaacatc tcggcggtcc agcggccgat
     5941 gccgcggatg gccaccagtt cctcgatgat ggcctcgtcg tccatgccgt gccacaggcc
     6001 cacgtgcacg gcgccgctgc tgaagttgtg cgccaggtcg cgcaggtact cgaccttgcg
     6061 cgccgacagc ccgacgccgc gcagcgcctc ggccgccagc ccgagcacgg ccgcgggctg
     6121 cagcagttcg acgtcgggac cgtcgacggc cccgaccagc ttgcgccaga cgccctgcgc
     6181 cgacttggtc gacacctgct ggccgacgat cgagcgcgcc agcgtcgtga acgcgtcgcc
     6241 gttgctctgc aggcgcgcgt cgccgaactg ggggatcagc ttcctcatca cccggtcgcg
     6301 cttgaccagg tggcggcagg cgtcgtccca gtaggcgggc acgtcgcggg cggcgtcgga
     6361 agcggcttga gcggccgcgg gactcgccat gtcaggcctt cacccaggtg gtgccttcgg
     6421 gtccgtcctt gagcgtgatg cccaggtccg cgagctcctg gcggatgcgg tcggactcgg
     6481 cgaagtcctt gttcttcttg gcctgggcgc gggcctcgat gcgggcctgg atctcggcgt
     6541 ccgtcagcgc ggcgccggcc tgcaggaagg ccgtcggctc ctgctgcagc acgcccagcg
     6601 tggcgccaag ggcgcgcagc agcgccgccg tctcgacgga cttcgtgcgg ttcaggtctg
     6661 tgaccatctc gaacagcacg gccagcgcgc ccggcgtgtt gaagtcgtcg ttcatcgcgt
     6721 cgcggaaggc cgcggcgcgc gggtcggtcc agtcgatggc ggcggcgtcg accggctgct
     6781 cggtcgcccc ggccgcagcg tgctccgggt gcaggccgac cgaggccagc gcggtgtaca
     6841 ggcggcgcag cgcggagcgc gcgtcgtcca ggtgcgcatc gctgaagttg aacgggctgc
     6901 ggtagtgcgt gcgcagcatg aagaagcgca gcgtctcgcc gtcgaacttc ttgaggacgt
     6961 cgcggatggt gaagaagttg ccgagcgact tcgacatctt ctcgttgtcg acgttcagga
     7021 agccgttgtg cagccagtag ttgacgaacg gatggcccag cgcgccctcg ctctgggcga
     7081 tctcgttctc gtggtggggg aactgcaggt ccatgccgcc gccgtggatg tcgaagcgtt
     7141 cgccgagcac ggcgcacgac atcgcggagc actcgatgtg ccagcccggg cggcccgggc
     7201 cgtacgcact gtcccacttg gcgtcgtcgg gctcggacgg cttggccgac ttccacagca
     7261 cgaagtccag cgggtcggtc ttgccgtcgg caacggccac gcgctcgccc gcgcgcaggt
     7321 cgtccagcga cttgcccgac agcttgccgt aacccgcgaa cttgcgcacc gcgtagttca
     7381 cgtcgccgtt gtcgccctgg taggccaggc ccttgtcccg cagcacgtcg atcatggcca
     7441 gcatctgcgg cacgtactcg gtggcgcgcg gctcgtgcgt gggcggctcg atgccgatgg
     7501 cgccgatgtc ctcgcgcatg gcggcgatca tctcgtcggt cagctggcgg atggtgatgc
     7561 cgcgctcgac cgcgcgcttg atgatcttgt cctcgatgtc cgtgatgttg cggacgtacg
     7621 tgacctcgta gcccagcacc tgcagccagc gatagacgac gtcgaacgcc atcatcatgc
     7681 gggcgtggcc catgtggcac aggtcataga tcgtcatgcc gcacacgtac atgcgcacgc
     7741 ggccagggac gatcggaacg aattcttcgg cttggccggt gagggtgttg aagagacgca
     7801 gcgtcatcag gaacaggagc cggcggcgaa ggcttcggcg aggaagcggc gggccgccgg
     7861 acgcggagat tggaggagag accgggtctt tcgcggcagg agcggcgccg cggtcgaaga
     7921 acggaagagt acgcggggtc cgggggcagg cgggctcggc tgccctcggt agaatcgggg
     7981 aagtatagcc aaggagacct ttggatgagc atcccccaac aaacccggtg ggccgcgctg
     8041 gccctggccg gcctgagcgc cttcgccgcc ccggcgttcg ccggcgacaa ggtgcacctg
     8101 gacaccacgc tgggcgagat cgtcgtcgaa ctcgacgcgg acaaggcgcc caagacggtg
     8161 gccaacttcg agcagtacgt caaggaaggc cactacaacg gcaccgtgtt ccaccgcgtg
     8221 atcccgaact tcatgatcca gggcggcggc atggtctcca ccttcgccgg cctgaaggaa
     8281 aagcccacgc acaagccgat cgtcaacgag tcgcacaacg gcctgagcaa cacgacgggc
     8341 accatcgcga tggcccgcac cggcgacccg aattccgcca ccgcgcagtt cttcatcaac
     8401 gtcaaggaca atccgttcct tgaccagggc gacggctacg ccgtgttcgg ccatgtcgtc
     8461 tcgggcatgg acgtcgtcga gaagatcaag atggtcgaga ccacgaccaa gggcccgttc
     8521 gagaacgtgc ccgtcacccc cgtcatcatc aagaaagcca ccctggagaa ataacatgac
     8581 caagtacgtg aagatgacca ccacgcaggg cgacctgcgc atcgagctgg acgacgccaa
     8641 ggcgccgatc tcggtcgcca acttcctgtc ctacgtgaac aagggcttct acgacggcac
     8701 gatcttccac cgcgtgatcc gcaacttcat gatccagggc ggcggcttcg agtccggcat
     8761 gaagcagaag gacaccgacg cgcagatcca gaacgaggcc aagaacggcc tgaagaacga
     8821 caagtacacg ctggcgatgg cgcgcacctc ggcgccccac tcggccaccg cgcagttctt
     8881 catcaacacc accaacaacg gcttcctgaa caacgaccag gcgcaggacg gctggggcta
     8941 cgcggtgttc ggcaaggtcg tccagggcca ggaggtcgtc gacgcgatcg gcggcgtgaa
     9001 gaccggccgc aagggcttcc acgacgacgt gccgctggag gacgtcaaga tcgtcaaggc
     9061 cgaggaagtc caggcttgag cttggcggcg acctttgatg cggaactgaa gctcggcgac
     9121 gacatccgcg tcgtcgactt catctccgac atccacctga gcgagaccat gccggccacg
     9181 gccgacgcat gggaagccta catggcgcgc acgcaggccg gcgcggtctt cctgctgggc
     9241 gacatcttcg aagtgtgggt cggcgacgac gcgcgcacgc gcccgttcga ggcgcgctgc
     9301 accgccgcca tcgccgccac cgccgcgcgc gtgccggtgt ggctgcagca cggcaaccgc
     9361 gacttcctga tcggcgccga gttcgcgcag gccacgggca tccgcctgct ggccgatccg
     9421 gcctcggtga cggcgttcgg ccgcacggtg ctgctc
//
LOCUS       DATCRO010000250         7912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_452710, whole genome shotgun sequence.
ACCESSION   DATCRO010000250 DATCRO010000000
VERSION     DATCRO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452710"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VIN75_06785"
     CDS             complement(<1..1251)
                     /locus_tag="VIN75_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8973905.1"
                     /translation="MSEKKIEFYKGPAGGWGALKSVSKALLSQDIPVKGARTLLSANQ
                     PDGFDCPGCAWPDRNHASTFEFCENGAKAVASEATARRVTSEFFAQHTVAELAGRSDF
                     FLEDQGRLTEPMVYDAASDKYRPIAWDDAFALVGRHLNALPDPNQAMFYTSGRASNEA
                     AFLYQLFVREYGTNNFPDCSNMCHEPSGSAMRPQIGVGKGTVTLDDFEQADAIFIFGQ
                     NPGTNHPRMLGELRAAAKRGARIVSFNPLRERGLERFADPQDKLEMATLGSTRISTHY
                     FQLRVGGDFALLKGMMKHVVEQEDARGGVLDHAFIAEHTVGVDALLADLRAESWPLLE
                     QESGLSEAQMRAAGDVYLQAERVIACWGMGITQHQHSVATIQMINNLLLLRGNIGRPG
                     AGPCPVRGHSNVQGDRTMGIWEKPP"
     gene            complement(1417..2430)
                     /locus_tag="VIN75_06790"
     CDS             complement(1417..2430)
                     /locus_tag="VIN75_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8973906.1"
                     /translation="MNALSREKACRLASYFLAGIALVGVLQLHLLVPLLVGLLVYQLV
                     VSVTPFAQRWFNTRLAKMLTVLLFATVIIAVIAGAVFGLIAFFRSDVENLPRLLAKVS
                     DILDKVRAGIPPFLAGYLPDDIADLQRKALEWLRTHVAELQMMGAHTLVTFAETIIAM
                     VIGVVLSLREVDPHATSGPLAIELTRRAERFADAFRNVALSQLSISLLNTTFTGLYLV
                     VGLHLFGVHLPLTKTMIAITFIVGLLPIVGNLTSNTIVVVVSLAHSPAVAVASLVFLI
                     VIHKLEYFLGARIVGTRIKSQIWELLIAMMALEALFGVPGLVAAPIYYAYLKSELIAA
                     RLI"
     gene            complement(2465..3193)
                     /locus_tag="VIN75_06795"
     CDS             complement(2465..3193)
                     /locus_tag="VIN75_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8973907.1"
                     /translation="MASPLLKVEHLHAGYGRAEVLSGIAVEAAAGSVVAVVGPNGAGK
                     STLLNALMGVLPARGEVRFDGEPVQRLSLEERVQRGIALVPETRALFASMPVADNLLL
                     GGYRQRRAPRAEQVARLDEIYALFPRLHERQAQAAGTLSGGERQMLAIGRALMGRPRL
                     LMLDEPSLGLAPLVVKEIFRIIERLRETGATILLIEQNARAAMAVADHAYVLETGAIA
                     LSGPAAQLAADPRVIETYLGKAKA"
     gene            complement(3193..4971)
                     /locus_tag="VIN75_06800"
     CDS             complement(3193..4971)
                     /locus_tag="VIN75_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY8973908.1"
                     /translation="MTNFPSLKIAAAAGVAALLALPLLPVPEFWITQLDYIGLYAVVV
                     LGLVLLTGVAGLTSFGQAAFVGLGAYASAYVTTAHGASPWLGLLCGLALTSVVALVVA
                     GVTLRMSGHYLPLATIAWGLAMFFLVGNLDFLGKFDGIQGIPPVAVGGVELRTGRASY
                     GLIWIVALLGALALRHLLDSRPGRAIRALKGGTLMAEAMGVGTLRYKMTAFVIAAIYA
                     SLSGWLFAHYQRSVNPSPFGLQMGIEYLFMAVLGGVGLVGGAFVGAAAVKVAEDQLQN
                     WLPALLGTSGPVELVVFGMVLILVLQVTPQGLWSLATRRMQVRKLDPSAWTGAEPLPR
                     ATPPARGETVLAVTQARKQFGGLVAVNDVSFDVRAGEIVGLIGPNGAGKSTLFNLITG
                     VLPLTRGQVDFRGARVSGLASRAIARRGMSRTFQHVRMIPDMSVLENVALGAHRRAGA
                     GVLRAMLRLDRREERRLLAEAWRQLDRIGMAALASEPAGNLALGQQRLMEIARALCTD
                     PSLLLLDEPAAGLRLKEKQALGQVLSQLRAEGMSLLLVEHDMDFVMTLTDRIVVVEFG
                     TKLTEGTPDQVRENPAVRAAYLGVEH"
     gene            complement(4979..6019)
                     /locus_tag="VIN75_06805"
     CDS             complement(4979..6019)
                     /locus_tag="VIN75_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8973909.1"
                     /translation="MDSTIAAVLLLDGIGNGAVYALLGMAIVLVFAVTRVILIPQGEF
                     VAYSALTLAMLQLGRVPGTVWLALLLAALAVLRETWDGWRTKRAWSATGLGIGRLCAL
                     PVAAAALTYVASGHAWPLLAQGLLTLVLVTSLGPLVYRLAYLPLADASVLLLLIVSVG
                     VHFTLVGLGLVFFGAEGYRTPPFWDATFNTGPVTISGQTIVTVLASALLMGALYVFFA
                     RTLAGKALRATAINRLGARLMGVSTEHAGRSAFSMAAFIGALSGLLIGPTTTMIYDGG
                     FLIGLKGFVAAIFGGLASYPLAVGGALLVGLLDSFSSFWASAYKDAIVFTAIIPILLL
                     RSLTDRHRDDDE"
     gene            6170..6559
                     /locus_tag="VIN75_06810"
     CDS             6170..6559
                     /locus_tag="VIN75_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973910.1"
                     /translation="MENNLGTVTVWQSGRLAPHVVTPRLDITRVTREQWIGAALLFLS
                     GVVMAVYVSVLQRDVSNAEMEHMAQRSRAVAEARCEADRPAERRGQCLALFNGDVAAL
                     DAAPTQAQIGDAREGAGRAMTVSLVGR"
     gene            6568..7056
                     /locus_tag="VIN75_06815"
     CDS             6568..7056
                     /locus_tag="VIN75_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8973911.1"
                     /translation="MEATDYLTRQHRDIEQALRDALDAGDAAVRRSRFLDIADALILH
                     IASEEQVFYPAVHVDRNEDVLLESLEEHLAIKRLVADLLALAGDDVTFQPKLHVLREL
                     AQHHHGDEERKLFPAVRGRLDAAAREALGREMEALQLRLQAGGKPRRAVRQQTAAAEP
                     LR"
     gene            complement(7064..7771)
                     /locus_tag="VIN75_06820"
     CDS             complement(7064..7771)
                     /locus_tag="VIN75_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973912.1"
                     /translation="MAVRLPAILASVPLFLAAHAVPATADLTPTEQRWLDGATPPLSW
                     AIRQGLPIDIVVLPQAEPGAAPIAMGWVDGRCKLVFAMRGNPAAESTLESIPAPLLQA
                     TLEAMTAHEIGHCQRHRSGTFDSLPAGLSDKPDAIESRQPTAELQAMAREMRITRREE
                     AFADLVGLAWTHAHHADQFPQVLAWFDAARSDETPRGFHDTRHWLALAHAPAAFAGDA
                     SPIEQAATLWQRGLLDD"
BASE COUNT         1215 a         2751 c         2889 g         1057 t
ORIGIN      
        1 gggcggcttc tcccagatgc ccatcgtgcg gtcgccctgc acgttgctgt ggccgcgcac
       61 cgggcacgga ccggcgccgg gccggccgat gttgccgcgc agcagcagca ggttgttgat
      121 catctggatc gtcgccaccg agtgctggtg ctgggtgatg cccatgcccc agcaggcgat
      181 gacgcgctcg gcctgcaggt agacgtcgcc ggccgcgcgc atctgcgcct cggacagccc
      241 ggactcctgt tccagcagcg gccaggactc ggcgcgcagg tcggccagca gcgcgtcgac
      301 cccgacggtg tgctcggcga tgaacgcgtg gtccagcacg ccgccgcgcg cgtcctcctg
      361 ttcgacgacg tgcttcatca tgcccttgag cagcgcgaag tcgccgccga cgcgcagctg
      421 gaagtagtgc gtgctgatgc gggtcgagcc gagcgtggcc atctcgagct tgtcctgcgg
      481 gtcggcgaag cgttcgaggc cgcgctcgcg cagcgggttg aagctgacga tccgcgcacc
      541 gcgcttggct gccgcgcgca gctcgcccag catgcgcggg tggttggtgc cggggttctg
      601 gccgaagatg aagatcgcgt cggcctgctc gaagtcgtcc agcgtgacgg tgcccttgcc
      661 cacgccgatc tgcggccgca tcgccgagcc gctgggctcg tggcacatgt tggagcagtc
      721 gggaaagttg ttggtgccgt actcgcgcac gaacagctgg tacaggaagg cggcctcgtt
      781 gctggcgcgg cccgacgtgt agaacatcgc ctggttcggg tcgggcaggg cgttcaggtg
      841 ccggccgacc agcgcgaacg cgtcgtccca ggcgatcggc cggtacttgt cgctcgcggc
      901 gtcgtagacc atgggctcgg tcagccggcc ctggtcctcg aggaagaagt cgctgcggcc
      961 ggcgagttcc gcgaccgtgt gctgcgcgaa gaattcggac gtcacgcggc gggcggtggc
     1021 ctcggacgcg acggccttgg cgccgttctc gcagaactcg aaggtggacg cgtggttgcg
     1081 gtcgggccag gcgcagcccg ggcagtcgaa gccgtcgggc tggttggccg acagcaaggt
     1141 cctggcgccc ttgacgggga tgtcctgcga cagcagcgcc ttgctcacgc tcttcaatgc
     1201 accccagccg ccggccgggc ccttgtagaa ctcgatcttc ttttcgctca ccgaaacgct
     1261 cttcccgggg tggttaagtg gaaacccgcg atgggaagtt tgcccgactc ggcggccgct
     1321 gaaaatatgc gctgcagtgc aacagccgcc caattccggc gattgacggc cgtcgggcag
     1381 gcgctcccgc gcgtgcccgg ccgacctgac gtccgttcag atgagcctgg cggcgatcag
     1441 ttcgctcttg aggtacgcgt agtagatcgg cgccgccacg aggccgggca cgccgaacag
     1501 cgcttccagg gccatcatcg cgatcagcag ctcccagatc tgcgacttga tgcgcgtgcc
     1561 gacgatgcgc gcgcccagga agtactccag cttgtggatc acgatcagga acacgagcga
     1621 ggcgacggcc accgccggcg aatgggccag gctgaccacc accacgatgg tgttggacgt
     1681 caggttgccg acgatcggca gcaggccgac gatgaaggtg atggcgatca tcgtcttggt
     1741 cagcggcagg tgcaccccga acaggtgcag gccgacgacc aggtacaggc ccgtgaaggt
     1801 ggtgttcagc agcgagatcg acagctgcga cagcgcgacg ttgcggaacg cgtcggcgaa
     1861 gcgctcggcg cgccgcgtca gctcgatggc cagcggcccg gacgtggcgt gggggtcgac
     1921 ctcgcgcagc gacagcacca cgccgatcac catcgcgatg atggtctcgg cgaaggtcac
     1981 cagcgtgtgc gcgcccatca tctgcagctc ggccacgtgc gtgcgcagcc actcgagcgc
     2041 cttgcgctgc aggtcggcga tgtcgtcggg caggtagccg gccaggaacg gcgggatgcc
     2101 ggcgcgcacc ttgtcgagga tgtcggacac cttggccagg agccgcggca ggttctcgac
     2161 gtcgctgcgg aagaacgcga tcagtccgaa cacggcgccc gcgatgaccg cgatgatcac
     2221 ggtggcgaac agcagcaccg tcagcatctt ggcgagccgg gtgttgaacc agcgctgcgc
     2281 gaacggcgtg acggagacga ccagctggta gaccagcagt cccaccagca gcggcaccag
     2341 caggtgcagc tggaggacgc cgaccagggc gatgccggcc aggaagtagg aagcgaggcg
     2401 gcaggctttt tcgcgcgaga gggcgttcat tgtgtcgttg aggtgggggc ggtggtcggc
     2461 cggatcaagc cttggccttg ccgaggtagg tttcgatgac gcgcgggtcg gcggccagct
     2521 gcgccgcggg gcccgacagc gcgatggcgc cggtctccag cacgtaggcg tggtcggcca
     2581 ccgccattgc ggcgcgggca ttctgctcga tcagcaggat ggtggcgccc gtctcgcgca
     2641 gccgctcgat gatgcggaag atctccttga ccaccagcgg cgcgagcccg aggctgggtt
     2701 cgtcgagcat cagcaggcgg ggccggccca tcagcgcgcg gccgatcgcc agcatctggc
     2761 gctcgccgcc cgacagcgtg ccggccgcct gggcctggcg ttcgtgcagg cgcgggaaca
     2821 gcgcgtagat ctcgtcgagc cgcgcgactt gctcggcgcg cggcgcgcgg cgctggcgat
     2881 agccgcccag cagcaggttg tcggccaccg gcatcgacgc gaacagggcg cgcgtctcgg
     2941 gcaccagcgc gatgccgcgc tgcacgcgct cctccagcga caggcgctgc accggctcgc
     3001 cgtcgaagcg cacctcgccg cgcgcgggca gcacccccat caatgcgttc agcagcgtgc
     3061 tcttgccggc gccgttgggc ccgacgaccg cgacgacgct gccggcggcg gcctcgacgg
     3121 cgatgcccga caacacctcc gcgcggccgt agccggcgtg caggtgctcg accttcagga
     3181 gcgggctggc catcagtgct ccaccccgag gtaggcggcg cgcacggccg ggttctcgcg
     3241 cacctggtcg ggcgtgcctt ccgtcagctt ggtgccgaac tcgaccacca cgatgcggtc
     3301 cgtcagcgtc atcacgaaat ccatgtcgtg ctccaccagc agcaggctca tgccctcggc
     3361 gcgcagctgc gacagcacct ggccgagcgc ctgcttctcc ttcaggcgca ggcccgccgc
     3421 gggctcgtcc agcagcagca gcgacggatc cgtgcacagc gcgcgggcga tctccatcag
     3481 gcgctgctgg cccagcgcca ggttgccggc cggctcgctg gccagcgccg ccatgccgat
     3541 gcgatcgagc tggcgccagg cctcggcgag caggcggcgt tcctcgcggc gatccaggcg
     3601 cagcatcgcg cgcagcacgc cggcccccgc gcgccggtgc gcgccgagcg cgacgttctc
     3661 cagcacgctc atgtccggga tcatgcgcac gtgctggaag gtgcgcgaca tgccccggcg
     3721 cgcgatcgcg cgggacgcga gcccgctcac gcgcgcgccg cggaagtcga cctggccccg
     3781 ggtgagcggc agcacgccgg tgatgaggtt gaacagcgtg gacttgcccg cgccgttcgg
     3841 gccgatgagt cccacgatct cgccggcgcg cacgtcgaag ctgacgtcgt tgacggccac
     3901 gaggccgccg aactgcttgc gcgcctgcgt caccgccagc accgtctcgc cgcgcgcggg
     3961 cggcgtcgcc cgcggcagcg gctcggcgcc ggtccaggcc gacggatcga gcttgcgcac
     4021 ctgcatgcgg cgcgtggcga gcgaccacag gccctgcggc gtgacctgca gcaccaggat
     4081 cagcaccatg ccgaacacca cgagttccac cggcccgctg gtgcccagca gcgccggcag
     4141 ccagttctgc agctggtcct cggccacctt gaccgcggcc gcgccgacga acgcgccgcc
     4201 gaccagcccc acgccgccca ggaccgccat gaacaggtac tcgatgccca tctgcagccc
     4261 gaacggcgac gggttcaccg agcgctggta gtgcgcgaac agccagcccg acaacgacgc
     4321 gtagatcgcg gcgatgacga aggccgtcat cttgtagcgc agcgtgccca cgcccatcgc
     4381 ctcggccatc agcgtcccgc ccttgagcgc gcggatggcg cggccggggc gcgagtcgag
     4441 caggtggcgc aacgccagcg cgcccagcag cgcgacgatc cagatcagcc cgtacgacgc
     4501 gcggcccgtg cgcagctcga cgccgccgac cgccaccggc gggatgccct ggatgccgtc
     4561 gaacttgccc aggaagtcga ggttgccgac caggaagaac atcgccaggc cccacgcgat
     4621 ggtggccagg ggcaggtagt gtccggacat gcgcagcgtc acgcccgcca ccaccagcgc
     4681 caccaccgat gtcagcgcga gcccgcacag caggcccagc cacggcgacg cgccgtgcgc
     4741 ggtcgtcacg taggcgctgg cgtaggcgcc caggccgacg aacgcggcct ggccgaagga
     4801 cgtgagcccg gccacgccgg tcagcagcac caggcccagc acgaccaccg cgtacaggcc
     4861 gatgtagtcc agctgcgtga tccagaactc gggcaccggc agcagcggca gcgccagcag
     4921 cgccgcgacg ccggccgcgg cggcgatctt gagggaagga aagttcgtca cggccggctc
     4981 actcgtcgtc gtcccggtgg cggtcggtga gcgagcgcag cagcaggatc gggatgatcg
     5041 cggtgaacac gatcgcgtcc ttgtacgcgc tggcccagaa cgagctgaac gagtccagca
     5101 ggcccaccag cagcgcgccg cccacggcca gcggatagct cgcgaggccg ccgaagatcg
     5161 cggcgacgaa gcccttcagg ccgatcagga agccgccgtc gtagatcatc gtcgtcgtcg
     5221 ggccgatcag caggcccgag agcgcgccga tgaaggccgc catcgagaac gcgctgcgtc
     5281 cggcatgctc ggtcgagacg cccatcaggc gcgcgccgag ccggttgatg gcggtcgcgc
     5341 gcagcgcctt gcccgccagc gtgcgcgcga agaagacgta cagcgcgccc atcaggagcg
     5401 cggaggcgag caccgtcacg atggtctggc ccgagatcgt gaccgggccc gtgttgaacg
     5461 tcgcgtccca gaacggcggc gtgcggtagc cctcggcgcc gaagaacacg aggcccaggc
     5521 cgaccagcgt gaaatgcacg cccaccgaca cgatcagcag cagcagcacc gaggcgtcgg
     5581 ccagcggcag gtaggccagg cgatagacga gcggtccgag cgaggtgacc agcaccagcg
     5641 tcagcaggcc ctgcgcgagc agcggccagg catgtccgct ggcgacatac gtgagcgcgg
     5701 cggccgcgac gggcagggcg cacagccggc cgatcccgag cccggtggcc gaccaggcgc
     5761 gcttcgtccg ccagccgtcc caggtctcgc gcagcacggc cagcgcggcc agcagcagcg
     5821 ccagccacac ggtgcccggc acgcgaccga gctgcagcat cgccagcgtc agcgcgctgt
     5881 aggcgacgaa ctcgccctgc gggatgagga tcacgcgcgt gaccgcgaac accagcacga
     5941 tggccatgcc cagcaaggca tacacggcgc cattgccgat gccgtccagc agcagcacgg
     6001 cggcgatcgt cgaatccatc cggtcctttc gtcagtcgtg gcacggcggg gtcggcgccg
     6061 tcgaactccc cgaatttacc ttgcccgacc gtgggcgcgt cgcgcgggcg cgagccttgc
     6121 cggaaacggg catcgcgcgg tccggcgccg cgatctggag aacgacatca tggagaacaa
     6181 cctgggcacc gtcaccgtct ggcaatccgg ccggctcgcg ccgcacgtcg tgaccccgcg
     6241 cttggacatc acgcgggtca cccgcgagca gtggatcggc gccgcgctgc tgttcctgtc
     6301 gggagtggtg atggccgtgt acgtgtctgt gctgcagcgc gacgtcagca atgccgagat
     6361 ggagcacatg gcgcagcggt cgcgcgccgt cgccgaggcg cgctgcgagg ccgaccggcc
     6421 cgccgagcgg cgtggccagt gcctggcgtt gttcaatggg gacgtcgccg ccctcgatgc
     6481 cgcgcccacg caggcgcaga tcggcgacgc gcgggaaggc gcgggacggg cgatgacggt
     6541 gtcgctggtc ggacgctgaa tcccgccatg gaggccaccg actacctgac gcggcagcac
     6601 cgcgacatcg agcaggcgct gcgcgacgcg ctcgacgcgg gcgacgcggc cgtgcgccgc
     6661 agtcgcttcc tggacatcgc cgacgcgctc atcctgcaca tcgcctccga ggagcaggtg
     6721 ttctatcccg cggtgcacgt cgaccgcaac gaggacgtgc tgctggagtc gctggaggag
     6781 cacctggcga tcaagcgcct ggtcgccgac ctgctcgcgc tcgccggcga cgacgtgacg
     6841 ttccagccca agctgcacgt gctgcgcgag ctggcgcagc atcatcacgg cgacgaggag
     6901 cgcaagctgt ttcccgcggt gcgcggcagg ctcgacgccg ccgcgcgcga ggcgctgggg
     6961 cgcgagatgg aggcgctgca actgcggctg caggccgggg gcaagccgcg gcgggcggtg
     7021 cggcagcaga cggcggcggc cgagccgctg cgctgaaggc ttctcagtcg tccagcaggc
     7081 cgcgctgcca gagcgtggcg gcctgttcga tcgggctcgc gtcgccggcg aaggccgccg
     7141 gcgcgtgcgc gagcgcgagc cagtggcgcg tgtcgtggaa gccgcgcggc gtctcgtcgg
     7201 agcgcgcggc gtcgaaccag gccagcacct gcgggaactg gtcggcgtgg tgggcgtgcg
     7261 tccaggcgag gccgacgagg tcggcgaagg cttcctcgcg gcgcgtgatg cgcatctcgc
     7321 gcgccatcgc ctgcagctcc gcggtgggct ggcgcgactc gatggcgtcg ggcttgtcgg
     7381 acaggccggc cggcaggctg tcgaaggtgc cgctgcggtg gcgctggcag tggccgatct
     7441 cgtgcgcggt catcgcctcg agcgtggcct gcagcagcgg ggcgggaatg gattcgagcg
     7501 tggactcggc ggccgggttg ccgcgcatcg cgaagaccag cttgcagcgg ccgtcgaccc
     7561 agcccatggc gatgggcgcg gcgccaggct cggcctgggg cagcacgacg atgtcgatcg
     7621 gcaggccctg cctgatcgcc caggacagcg gcggggtcgc gccgtccagc cagcgctgct
     7681 cggtcggcgt gaggtcggcc gtcgccggga cggcgtgggc ggccaggaac agggggacgg
     7741 acgcgaggat cgcgggaaga cgaacggcca cgacggctcc tcggtcaggg gcgtccggct
     7801 tacttctggc gggcctggcg gcaggacgcc atcgtgcgct ccagcagcga cggggtggag
     7861 gcttcggcca gcgccttgcg cagcatcttg gcgtgcaccc ggtagcgcag cg
//
LOCUS       DATCRO010000251         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_509285, whole genome shotgun sequence.
ACCESSION   DATCRO010000251 DATCRO010000000
VERSION     DATCRO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509285"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VIN75_06825"
     CDS             complement(<1..623)
                     /locus_tag="VIN75_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8973913.1"
                     /translation="MNLLDPAALESLAALAEAGSFERAAQLLSITQSAVSQRLRALET
                     QVGQLLVVRSRPLRMTEPGKVLLRYARQLQALRADAARELGAAQMREERLPIAINADS
                     LATWVLGALDALVHAGQREGYGLELIVDDQDFTHDRLREGEVLGCVSTVSMALRGCTC
                     QPLGRMRYIAVASPGFVATAMPEGLTPANFARLPFIVFNRKDDMQAQ"
     gene            726..1841
                     /gene="ald"
                     /locus_tag="VIN75_06830"
     CDS             726..1841
                     /gene="ald"
                     /locus_tag="VIN75_06830"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY8973914.1"
                     /translation="MQIGVPKEIKNHEYRVGLTPASVRDYVAHGHAVMVQAGAGAAIG
                     LDDAQYRAAGAAIVDTAQEVFARADMVVKVKEPQPQECAMLRPGQLLYTYLHLAPDPE
                     QTAALVKSGAICVAYETITGANGGLPLLAPMSEVAGRMSIQAGAAHLEKSKGGMGVLL
                     GGVPGVAPAHAVILGAGVVGTHALQCAVGAGARVTVLDKNVDRLRQLDLVFGNRITTL
                     YSNAQSVEDSVLAADLVVGGVLIPGAAAPKLVTRDMISRMKKGAVVVDVAIDQGGCFE
                     TSHATTHAEPTYVVDGVVHYCVANMPGAVARTSTFALNNATIGPGLALADQGWQAAMR
                     ANPHLKAGLNVASGKVTFEAVAQALGYDYTPADALLN"
     gene            1918..>2327
                     /locus_tag="VIN75_06835"
     CDS             1918..>2327
                     /locus_tag="VIN75_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY8973915.1"
                     /translation="MSRLAVLPQYVYPKRLLTNFAGWVAGKERGRITTRLIRNFIRDY
                     DVDMDEALESDPAAYKTFNAFFTRALKPGARPLANADLICPVDGAISQFGRINTHTIF
                     QAKGHEYTTQALLGGDPALADPFLDGSFATIYLSP"
BASE COUNT          367 a          826 c          813 g          321 t
ORIGIN      
        1 cattgcgcct gcatgtcgtc cttgcgattg aagacgatga acggcagcct ggcgaagttc
       61 gccggcgtca ggccttcggg catcgcggtg gcgacgaagc ctgggcttgc gacggcgatg
      121 tagcgcatgc gcccgagcgg ctggcaggtg cagccgcgca gcgccatcga gaccgtggac
      181 acgcagccga gcacctcgcc ctcgcgcagg cggtcgtgcg tgaagtcctg gtcgtcgacg
      241 atcagctcca ggccgtagcc ctcgcgctgg cccgcgtgca ccagcgcatc gagcgcgccc
      301 agcacccagg tggccaggct gtccgcgttg atggcgatgg gcaggcgctc ctcgcgcatc
      361 tgcgcggcgc cgagctcgcg cgcggcatcg gcgcgcagcg cctgcagctg gcgcgcatag
      421 cgcagcagca ccttgccggg ctcggtcatg cgcagcggcc gcgagcgcac caccagcagc
      481 tggccgacct gcgtctcgag cgcacgcagg cgctgcgaga cggccgactg cgtgatcgac
      541 agcagctgcg cggcgcgctc gaaactgccc gcctccgcca gggcggccag cgactcgagc
      601 gcggcggggt cgagcaggtt cataagtaac gctactgtaa tcgctgcaat ttcgatgaat
      661 cttcacactt tccctcggcc ggcctacgct ggatcgacca cgaaaacgtc ccattggaga
      721 caaccatgca gatcggcgtt ccgaaggaga tcaagaacca cgagtaccgc gtcggcctga
      781 cgcccgccag cgtgcgcgac tacgtcgccc acggccacgc ggtgatggtg caggcgggcg
      841 ccggcgcggc gatcggcctg gacgacgcgc agtaccgcgc ggcgggcgcg gcgatcgtcg
      901 acacggcgca ggaggtgttc gccagggccg acatggtcgt caaggtcaag gagccccagc
      961 cgcaggagtg cgcgatgctg cgtcccggcc agctgctcta cacctacctg cacctggcgc
     1021 cggatccgga gcagaccgcc gcgctggtca agtcgggcgc gatctgcgtc gcctacgaga
     1081 ccatcaccgg ggccaacggc ggcctgccgc tgctcgcacc gatgagcgag gtcgcggggc
     1141 gcatgtccat ccaggcgggc gccgcgcacc tcgagaagag caagggcggc atgggcgtcc
     1201 tgctgggcgg cgtgcccggc gtggcgccgg cgcatgccgt gatcctgggg gcgggtgtcg
     1261 tgggcacgca cgcgctgcag tgcgcggtcg gcgcgggcgc gcgcgtgacg gtgctggaca
     1321 agaacgtcga ccggctgcgc cagctcgacc tcgtcttcgg caaccgcatc acgacgctgt
     1381 actcgaacgc ccagtcggtc gaggactcgg tgctggcggc cgacctggtc gtcggcggcg
     1441 tgctgatccc cggagccgcg gcgcccaagc tggtcacgcg cgacatgatc tcgcgcatga
     1501 agaagggcgc ggtcgtcgtc gacgtcgcga tcgaccaggg cggctgcttc gagacctcgc
     1561 acgccaccac gcacgcggag cccacctacg tggtcgacgg cgtcgtccac tactgcgtgg
     1621 ccaacatgcc cggcgccgtg gcgcgcacgt ccaccttcgc gctcaacaac gcgacgatcg
     1681 ggccgggcct ggcgctggcg gaccagggct ggcaggcggc gatgcgcgcg aatccgcacc
     1741 tgaaggccgg cctgaacgtg gccagcggca aggtcacgtt cgaggccgtc gcgcaggcgc
     1801 tcggctacga ctacacgccg gccgatgcgc tgctgaactg acggcccggc agggcgggcg
     1861 agccggttgc ccgacacgtg acggcggcgc gacggccgcc ggataaaatt gccggtcatg
     1921 tcccgtctcg ccgtcctgcc tcaatacgtc tatcccaagc gcctgctcac gaacttcgcg
     1981 ggctgggtcg cgggcaagga gcgcgggcgc atcaccacgc gcctgatccg caacttcatc
     2041 cgcgattacg acgtcgacat ggacgaggcg ctggagtccg acccggcggc ctacaagacc
     2101 ttcaatgcgt tcttcacgcg cgcgctcaag ccgggcgcgc ggccgctcgc gaacgccgac
     2161 ctgatctgcc cggtggacgg cgcgatcagc cagttcggcc gcatcaacac gcacacgatc
     2221 ttccaggcca agggccacga gtacacgacg caggcgttgc tgggcggcga cccggcgctt
     2281 gccgacccgt tcctcgacgg cagcttcgcc acgatctacc tcagccc
//
LOCUS       DATCRO010000252         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_250195, whole genome shotgun sequence.
ACCESSION   DATCRO010000252 DATCRO010000000
VERSION     DATCRO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250195"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..509)
                     /locus_tag="VIN75_06840"
     CDS             complement(84..509)
                     /locus_tag="VIN75_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY8973916.1"
                     /translation="MTALTRFDDIDNFFPEFFRRLARPLALDAGLSADMRLDVSENEK
                     EYLVSAEIPGARKDDIRVQIDRNTVTITADVRKDIEDKHANGGRALLRETFRGHTMRS
                     FTLGQEVDDKAAVAKLDDGVLRLTLPKRPGSTGKLLTIQ"
     gene            792..1445
                     /locus_tag="VIN75_06845"
     CDS             792..1445
                     /locus_tag="VIN75_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8973917.1"
                     /translation="MKSDVQIKADVEEELAWDPVVDEAHVGVSVHDAVVELTGTLGSH
                     AEKMAAEHAALRVSGVQGVTLAISVAPRAGFERADADIVQACQHALAWSSRVPEGAVR
                     VQVEKGHVTLVGEVDWAYQRKAAETAVWDLLGVVSVVNEIRVRSRVGAADIRAGIQRA
                     LERQADREARRIDVHVDGSCVALAGRTRTWAEREAAVAAAWAAPGVTRVVNEIVVGP"
     gene            1486..3987
                     /locus_tag="VIN75_06850"
     CDS             1486..3987
                     /locus_tag="VIN75_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973918.1"
                     /translation="MNPGRDFIRGAPPPSPGLIALWAGLLSDAGVMVAVIAADGGVLL
                     ANRALRAACHWPIDDASSRSWRDCLADDDPAAVEGFARWISADVPPGPVEVRLRDGVQ
                     GVGRWLRWYRARQDDEPGASATVALVGFDVSREHEIDAKLRRSARFYRALSDMNAAMG
                     RLRDAPSLYQAACDIAVVSGGAHMARVGLPQGDCLVPVAWGGSARRYTAGLCIRIDAD
                     HGELRGPSSEAFLTEVPCICNDVESDPRMAPWRERARAAGVRASGAFPIVRNGACAGV
                     LNLYFSEPDAFDEQLVDLARRMASDVEFALDKIDREAARADAERIASERDLQLSGLVE
                     SALDAIIAIDVGQRVVLFNAAAVRMFGVPADEAIGGTLDRFIPPAAREAHRRHVERYA
                     MEGATSRHMGSARELIGLRASGEVFPIEASISRSGEGARMLMTVMVRDVSQVRVAERE
                     QTARLQAEAASRAKTEFLSRMSHELRTPLNAVLGFSQLLKADTRDPLSMRQREQVELV
                     IQAGDHLRILIEEMLDFAGIEAGRVAIEPRDFELRSLLDGVLRMSAPHAQDSGVRLMA
                     DYPPDEAILMHSDPSRVRQIVLNLVSNAIKYNRRGGRVRLGVERVPGRVRIVVRDDGL
                     GMTPAQLSQLFQPFNRLGREWGTTPGMGIGLVLVRQLARLLGGDVDVQSASGSGTTVR
                     VSLPAAAASPPAAGDGQDRPVDADGNDVRGRVLYVEDNRVNAMLIEQMLARWPGVEVV
                     IADDGAAALAEAASLSPDVILLDLQLPDMSGLEVLRRLRSQASTKGVPIIALSASALP
                     DQVAAARAAGADDYWTKPVRLETFLGDMRKLLARP"
     gene            4089..4508
                     /locus_tag="VIN75_06855"
     CDS             4089..4508
                     /locus_tag="VIN75_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8973919.1"
                     /translation="MKMLLAVDGSDYSRRALDFLERSGWLRPDNEISVFTVVLPVPHA
                     AAAFVSPAVVANYYQDDAEQVLRPIRQRLVSCSAKVDFAHVIGIRADAIARKATSEGY
                     DLVVMGSHGHGALGNVVLGSVATRVLATCRTPVLLIR"
BASE COUNT          612 a         1560 c         1725 g          705 t
ORIGIN      
        1 gggctgacgc tgtcggcgac gttccggcga cgcccgtcat cgggtcgtcg ccggtgcggc
       61 gccaggtcga tcgaatgacc gccttactgg atggtcagca gcttgcccgt gctgccgggc
      121 cgcttcggca gggtcaggcg cagcacgccg tcgtccagct tggcgacggc ggccttgtcg
      181 tcgacctcct ggcccagcgt gaacgagcgc atggtgtggc cgcggaaggt ctcgcgcagc
      241 agggcgcggc cgccgttggc gtgcttgtcc tcgatgtcct tgcgcacgtc ggccgtgatc
      301 gtgacggtgt tgcgatcgat ctggacgcgg atgtcgtcct tgcgggcgcc ggggatctcg
      361 gcgctcacca ggtattcctt ctcgttctcg ctgacgtcca ggcgcatgtc ggccgacagg
      421 cccgcgtcca gcgcgagcgg gcgcgcgagg cggcggaaga attcggggaa gaagttgtcg
      481 atgtcgtcga atcgggtcag ggcagtcatg cggatctcct ggagtgaagg gatggattgc
      541 ggaccaccac tctaggaagg tgcgtggccc cggcgtttgc gcgcgctcaa tctggcgatc
      601 gaacggcccg gaggtcgaat ggcttgcgca tgcgcaaggc gctcgcggcg ccaacgcgaa
      661 gaatggcctt cgcggattcg acccggcgcg tgcgccgcat gagggattcc cgtcgattcg
      721 ccggacgtcg gcgcgtggag ccgcgcgcgt cttgccgaac cttgatgcgc ggccattcca
      781 ggagaacagc gatgaagagc gatgtgcaga tcaaggccga tgtcgaggaa gagctggcgt
      841 gggatcccgt cgtggacgag gcgcacgtgg gcgtcagcgt ccatgatgcc gtcgtggagc
      901 tgaccggcac gctcggcagc catgccgaga agatggcggc cgaacacgcg gccctgcggg
      961 tcagcggcgt gcagggcgtg acgctggcga tcagcgtcgc ccccagggcg ggcttcgaac
     1021 gggccgacgc ggacatcgtg caggcgtgcc agcatgcgct cgcgtggagc tcgcgcgtcc
     1081 cggaaggcgc cgtcagggtg caggtcgaga aggggcacgt cacgctcgtc ggcgaggtcg
     1141 attgggcata ccagcgcaag gcggcggaga cggcggtctg ggacctgctc ggcgtcgtgt
     1201 ccgtggtcaa cgaaatccgc gtgcgctcgc gagtcggcgc ggccgacatc cgcgccggca
     1261 tccagcgggc gctcgagcgc caggcggacc gcgaggccag gcgcatcgac gtgcacgtgg
     1321 acggctcctg cgtcgcgctg gccggtcgca cgaggacgtg ggcggaacgc gaggcggccg
     1381 tcgcggccgc ctgggccgct ccgggggtga cgcgggtcgt caacgagatc gtggtgggcc
     1441 cgtgatgcct ggccacgccc gcccggccga cgtgcgcggg cttcgatgaa cccgggccgg
     1501 gatttcattc gcggcgcgcc gccgccgtcg ccgggcctga tcgcgctctg ggccggcctc
     1561 ctgtcggacg ccggcgtcat ggtggcggtg atcgccgcgg acgggggcgt actgctcgcc
     1621 aaccgggcgc tccgcgcggc ctgccactgg ccgatcgacg acgcgtcctc gcgatcctgg
     1681 cgcgactgcc tggccgatga cgacccggcg gccgtcgagg gattcgcccg ctggatatcc
     1741 gccgacgttc cgcccggtcc ggtcgaagtg cggttgcggg acggtgtcca aggggtgggc
     1801 cgctggctgc gttggtaccg cgcgcgccag gacgacgaac cgggcgcgtc cgccacggtg
     1861 gcactggtcg gcttcgacgt gtcgcgcgag cacgagatcg atgccaagct gcggcgctcg
     1921 gcgcgcttct accgcgcctt gtcggacatg aacgccgcga tggggcgcct gcgggatgcg
     1981 ccgtcgctgt accaggccgc gtgcgatatc gcggtcgtct ccggcggcgc gcacatggcc
     2041 cgggtgggcc tgccgcaggg cgattgcctg gtcccggtgg cgtggggcgg cagcgcgcgt
     2101 cgctacaccg cgggcctgtg cattcgcatc gacgccgacc atggcgagct gcgcgggccg
     2161 tcgagcgagg cctttctcac cgaggtcccg tgcatctgca acgacgtgga gtccgatccg
     2221 cggatggcgc cctggcggga acgcgctcgc gcggcgggcg tccgggcctc gggcgccttt
     2281 cccatcgtcc ggaacggcgc ctgcgctggc gtgctgaacc tctacttctc ggagcctgac
     2341 gccttcgacg agcaactggt cgaccttgcg cggcgcatgg cgtcggacgt ggagttcgcg
     2401 ctcgacaaga tcgatcgcga ggccgcccgt gcggacgcgg agcgcatcgc gtccgagcgg
     2461 gatctccagt tgtcggggct cgtcgaatcc gcgctggacg ccatcatcgc gatcgatgtc
     2521 ggccagcggg tggtcctgtt caatgccgcc gcggtccgga tgttcggcgt gcccgccgac
     2581 gaggcgatcg gcggaacgct ggatcgcttc atccctcccg ccgcgcgcga ggcccaccgg
     2641 cgtcacgtcg agcgctatgc gatggaaggc gccacctccc ggcacatggg ctccgcccgc
     2701 gaactgatcg gcctgcgggc gtccggcgag gtctttccca tcgaggcatc gatctcccga
     2761 tcaggcgagg gcgcgcgcat gctgatgacg gtgatggtgc gcgacgtcag ccaggtgcgc
     2821 gtcgccgaac gcgagcagac cgcgcgcctg caggccgagg cggcgagccg ggccaagacc
     2881 gagttcctgt cccgcatgag ccacgagctg cgcacgcccc tgaacgccgt gctgggcttt
     2941 tcgcaactgc tgaaggcgga cacgcgggac ccgctgtcga tgcgccagcg cgagcaggtc
     3001 gagctggtga tccaggccgg cgaccacctg cggatcctca tcgaggaaat gctggacttc
     3061 gcgggcatcg aggccggccg cgtggccatc gagccgcgcg acttcgagct gcgctcgttg
     3121 ctcgacggcg tcctgcgcat gagcgcgccg catgcccagg acagcggcgt ccgcctcatg
     3181 gccgactacc cgccggacga ggccatcctg atgcacagcg acccctcgcg ggtccggcag
     3241 atcgtgctga acctcgtctc gaacgcgatc aagtacaacc ggcgtggagg ccgcgtccgc
     3301 ctgggcgtcg aacgggtgcc ggggcgcgtg cgcatcgtgg tgcgcgacga cggcctcggc
     3361 atgaccccgg cgcagctgtc gcagctgttc cagccgttca accggcttgg ccgggagtgg
     3421 gggacgacgc ccggcatggg catcggcctg gtgctggtgc gccagctggc gcgcctgctc
     3481 ggcggcgacg tggacgtcca gagcgcgagc ggcagcggga cgaccgtccg ggtgagcttg
     3541 ccggccgccg cggccagccc gcccgcggcc ggcgacgggc aggatcgtcc cgtcgacgcg
     3601 gacggcaacg acgtccgtgg acgggtgctc tatgtcgagg acaaccgggt caacgcgatg
     3661 ctgatcgagc agatgctcgc gcgctggccc ggcgtggagg tggtcatcgc cgacgatggc
     3721 gcggcggccc tggccgaggc ggccagcctg tcgccggacg tcatcctgct ggacctgcaa
     3781 ctgcccgaca tgagcgggct ggaggtgctg cgacgcctgc gttcgcaagc ctcgacgaag
     3841 ggcgtcccga tcatcgccct gtcggcgagc gcgctgccgg accaggtcgc cgccgcgcgg
     3901 gccgcggggg cggatgacta ctggacgaaa ccggtgcggc tcgagacgtt cctgggcgac
     3961 atgcgcaagc tgctcgctcg cccctgaagc tgcgcggcct gcgcccgcat cggtgcttga
     4021 gctggcgcaa gcgccgcgcc gcggccgcgc cttacgctgg ccgcgacgcc cgacatcgga
     4081 gatgacccat gaagatgctg ctcgccgtcg acggcagtga ctacagccgc cgcgccctcg
     4141 acttcctgga gcgcagcggc tggctgcgcc cggacaacga gatctcggtg ttcaccgtcg
     4201 tcctgcccgt tcctcatgca gccgcggcgt tcgtctcgcc ggcggtggtc gcgaactact
     4261 accaggacga tgcggagcag gtgttgcggc cgatccgcca gcgcctggtg tcctgcagcg
     4321 cgaaggtcga cttcgcgcac gtcatcggca tccgcgcgga cgcgatcgcg cgcaaggcga
     4381 cgagcgaagg ctacgacctc gtcgtgatgg gatcgcacgg gcacggcgcg ctcggcaacg
     4441 tggtgctggg ctccgtcgcc acgcgggtac tggcgacgtg ccggacaccc gtgctgctga
     4501 tccgctgatc ttcctcccgg ccgtggccct ggtctggaag ctggtcgcga tcctctcgct
     4561 gttgctggcc gcgctcgcgg cggtcctgcc ggtggtgccg gc
//
LOCUS       DATCRO010000253        13421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_272910, whole genome shotgun sequence.
ACCESSION   DATCRO010000253 DATCRO010000000
VERSION     DATCRO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13421
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272910"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1236)
                     /gene="rtcA"
                     /locus_tag="VIN75_06860"
     CDS             complement(199..1236)
                     /gene="rtcA"
                     /locus_tag="VIN75_06860"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEY8973920.1"
                     /translation="MIEIDGSVGEGGGQILRTSLALSMCTGQPFTLARIRARRAKPGL
                     MRQHLTCVQAAAAVSGATVQGAELGSGMLVFEPGAVRAGEHAFSVGTAGSCTLVLQTV
                     WPALMLADAPSRLKLAGGTHNPMAPPFHFLQRCYAPLLRRLGAESELSLRRHGFYPAG
                     GGEIDVTVHPAAGTLQPFDLLERGEAVESYAECLAPALPGAVARRELDALARALNWTP
                     AQLRVPVVRQNEGPGNALMATLAHAHVCELFTQFGEKGVSSEQVAQALAKEVRDYRGS
                     TAALGPHLADQWALPLALAVCRRGTAAAYTCTELTDHATTNFQMIERFLPVRFETEAV
                     DGGWRVSLKLR"
     gene            complement(1469..1591)
                     /locus_tag="VIN75_06865"
                     /pseudo
     CDS             complement(1469..1591)
                     /locus_tag="VIN75_06865"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
     gene            complement(1654..2877)
                     /locus_tag="VIN75_06870"
     CDS             complement(1654..2877)
                     /locus_tag="VIN75_06870"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY8973921.1"
                     /translation="MEQNYDIEDVPGGVPVKMWTRGVPVEDDAKKQLANAARLPVVFK
                     HIAAMPDVHLGIGATVGSVIPTLKAIIPAAVGVDIGCGMMAARTTLRAQDLPDNLGPL
                     RSAIERAVPHGRSAKGWTRDEGAWENPPETVDQVWATLAGEFDALCELHPRLRNTNNR
                     KHLGTLGTGNHFIEVCLDEAGFVWFMLHSGSRGVGNAIGTHFIELAKKDALRNNANLP
                     DQDLAYFEEGAQYFGDYVRGVSWAQKFAARNREVMMANLIAVVRKVIPKPFESHVEAV
                     NCHHNYVQQERHFGEDVFVTRKGAVSARKGQMGIIPGSMGARSYIVRGLGNPESFDSC
                     SHGAGRVMSRTKAKKLFTVDDQIKATEGVECRKDADVIDEIPMAYKDIDAVMAAQRDL
                     VEIVHTLKQVVCVKG"
     gene            complement(3223..4821)
                     /locus_tag="VIN75_06875"
     CDS             complement(3223..4821)
                     /locus_tag="VIN75_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8973922.1"
                     /translation="MNKQGQRFMVNTQLFQTLRGALLPAPTATNASKAPAYAYTPRHQ
                     LAQLAATGCLGHTFHADAEAQLEAVLALAAQVEPGFVAKTAVHARQAGHMKDMPALLA
                     ATLAVRDVALLAQVFGRVVDNGKMLRDFVQILRSGATGRKSLGSRPKKLVQQWLLNAS
                     ERQLLNACVGNAPSLADVVKMVHPKPDEAWRAAWFAWVIGKDVELSALPPLTQAFERF
                     KRARAEGKGGELPDVPFQMLTALELSTGQWAQVARNGSWQMVRQNLNTFARHGVFQRR
                     RMVQLVAARLRDPAAIAKARVMPYQLLAAFKATGEGVPAEIRDALQDAMELAIANVPS
                     FAGRVVVCPDVSGSMSQAVTGHRGSATSSVRCIDVAALVAAAVLRKNPSARVLPFEQG
                     VVSLSLNGRDSVMTNAQALASIGGGGTNCSAPLALLNRERAEVDVVILVSDNESWVDA
                     NRRGATQTLREWEALKQRNPSARLVCIDLQPGATTQAAERHDILNVGGFSDAVFDLVA
                     SFARGRADADHWVGEIDKIPLAAQ"
     gene            5003..6601
                     /gene="rtcR"
                     /locus_tag="VIN75_06880"
     CDS             5003..6601
                     /gene="rtcR"
                     /locus_tag="VIN75_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HEY8973923.1"
                     /translation="MTKRTVVIGFLGTQLDAGQGAGRWEKWRPTLSLMQHEDTVIDRL
                     ELLHASAHIGLARQLKADIATVSPETAVNLVALDVADPWDFGEMYAGLYDWARGYRFD
                     PAHEQYWAHITTGTHVAQICMFLMVESRFIPGLLLQTAPPKKQRQGNAGSYTLIDLDL
                     SRYDALARRFDQAQAEALEFLKSGIPTRNARFNAMIEEIERVAVRSRAPILLTGPTGA
                     GKSHLARRMYELKKARHQVTGEFVEVNCATLHGDGAASTLFGHKKGAFTGAVTDRAGL
                     LRSAHEGVLFLDEIGELGLDEQAMLLKAIEEKRFLPVGSDRETGSDFQLIVGTNRDLR
                     RDVAAGRFRDDLFARINLWSYALPGLAQRPEDLEPNVDWLLARAVAESGHAVRFNVEA
                     KSRYLKFAQSPGAAWSGNFRDLSASVTRLATLADGGRISTALVDAEIARLQWLWERKP
                     AADAAAVDLAALLDAATLADIDLFDRLQLEAVIRTCRESATLSDAGRRLFDRSREKRS
                     VVNDADRLRKYLLKFGLAWEAVRA"
     gene            complement(6605..7006)
                     /locus_tag="VIN75_06885"
     CDS             complement(6605..7006)
                     /locus_tag="VIN75_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEY8973924.1"
                     /translation="MTSHTATGPFDVKIAPQPLSAVADGSGLARMSLDKRFHGALEAT
                     STGEMLSFGNPGAGSAGYVAMESVRGSLDGRAGSFALQHSSTLDHGVPTQSITVVPGS
                     GTDSLAGLAGRMVVEIAPGGAHSYRFDYTLP"
     gene            7197..7967
                     /locus_tag="VIN75_06890"
     CDS             7197..7967
                     /locus_tag="VIN75_06890"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8973925.1"
                     /translation="MLHRLALGEFAHGRFEAPDDLADRIEHFWTVRWNLDGLPPQVQE
                     TLPHPNVHLVIEPGSIAAWGVHTGRWTRVLEGRSQAFGVKFRPGAFRALLGRAVSTIA
                     DGSIDARAIFGADAAALAGVLGHDDDRDAAALASAFLRERLAPATDDALLAGRIVDSV
                     ADDIELHSADELARRFGMSLRALQRLFNQFVGVGPKWVIKRYRMHEAVARVQAGQPVS
                     WAALAQDLGYFDQAHFIADFRKLVGMTPGDYARERHPR"
     gene            complement(7974..9359)
                     /locus_tag="VIN75_06895"
     CDS             complement(7974..9359)
                     /locus_tag="VIN75_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8973926.1"
                     /translation="MSSLPPQRGDDPSLVDASRARLAQLRDALGVVGRGLPISLGLLL
                     LLSLIAAIVAFVFLRSAPPTTLVLTSGPKGSSFANVAEQYRKILAREGVTLKILTSQG
                     SRDNLARLEDGKQDVDVGFVVGGETTGHDTSHLVSLGSLSYQPMMIFYRGKPRTLISD
                     FKQHRLDIGLEGSGASALAQTLLDANGIKPGDGTQVGHRPPADSIQLLKEGKIDALFA
                     MSDSTPTALMRELLRDEDVHLFNFVQAEGYTHRITYLNKLVLPRGAIDFGEDIPPESV
                     TLIAPTVELIARDDLHPALSDLLLTAAREVHGRAGLYKKAGDFPSAIEHDFPISGDAS
                     RFYASGRSFLYRSFPFWIASLIERVLAIVVPMTIILVPGFKIAPAVFRWRVVSRIYRW
                     YAVLQRIERDADAGPLDAERCDALLARLEHVETTVTRLAVPPAFGDLLYGLRSHIAVV
                     RESLRARRAAL"
     gene            complement(9586..10257)
                     /locus_tag="VIN75_06900"
     CDS             complement(9586..10257)
                     /locus_tag="VIN75_06900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY8973927.1"
                     /translation="MTDTLTTAPVAPLLARLFAEADEAAIGLNAMAAALPPGELARLM
                     HSRTEYLDFYTRLKDQPLPVSRATGALLYMLARSTGAHAIVEFGTSFGLSTLHLAAAL
                     RDNGSGGRLITCEFEPSKVVRARANLAAAGLDDLVEIREGDALQTLARDLPGAIDLVL
                     LDGAKALYPEILALLEPRLRPGALVVADNADDSPEYLARVRSADGGYLSTPFGGDVEV
                     SMRLG"
     gene            10341..11021
                     /locus_tag="VIN75_06905"
     CDS             10341..11021
                     /locus_tag="VIN75_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8973928.1"
                     /translation="MTDRTTSRLSSRKRPRQARSTDLVAAILEAAIQVLSREGAQRFT
                     TTRVAERAGVSVGSVYQYFPNKAAILFRLQSDEWRETTDLLRATLADTSRPPLERLRR
                     LVHAFVKSECDEAAMRVALADAAPLYRDAPEAQEVHAASKGLLHAFMRETLPDAKPAT
                     RARAGRLIKATLSAVGHDFSGSPRSAREIAAYSDAMADMFAAYLVALGHLEPVPARGR
                     ARLARAAA"
     gene            complement(11027..12034)
                     /locus_tag="VIN75_06910"
     CDS             complement(11027..12034)
                     /locus_tag="VIN75_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8973929.1"
                     /translation="MTADTASMRALVLEAANTPFVSRQVARPVAGPGQVLVQVHASGV
                     NPLDSKIRAGAAAHARHPLPGVLGMDLAGVVRGVGAGVERWRVGDEVYGLTGGVAGLQ
                     GSLAEYVAVDADLLARKPANLSMREAAALPLIAITAWEGLVDRAGVQAGQKVLVHGGA
                     GGVGHAAVQIARALGAQVFATASAADADIVRRFGATPIDYVAATVDDYVGEHTAGRGF
                     DVVFDTVGGAVLDASFRAARTYGGHVVSALGWGTHSLAPLSFRAASYSGVFTLLPMLT
                     GERRAHHGEILEEVAKLVESGRLVPRMDARRYTLDTALEAHEEVAAGRARGKVVVEVQ
                     G"
     gene            12135..12986
                     /locus_tag="VIN75_06915"
     CDS             12135..12986
                     /locus_tag="VIN75_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973930.1"
                     /translation="MEWSDIRHFLAIARARSLNGAARLTGQSQPTMGRRLRALEHALG
                     HALFQRGSEGFVLTDEGAALLAHAERMEEEALAFERELAGQGEELDGQLRVSSSDWFG
                     VHMLAPVLAEFIKANPRVAIELVTDARLLNLARREADLAFRIQAFAEPDVIQRRLMTI
                     PYGLYAAQGRARALAKDGSGCALITLDAAYRDFPDARWLRQVLPKAHVAFTSNSREAQ
                     ARMCAEGVGLAVLPRPLGDGMAGLRLVRTDAPPPGRDVWVGFHRDLRRLRRLRALVDL
                     CVERLAA"
     gene            13143..>13421
                     /locus_tag="VIN75_06920"
     CDS             13143..>13421
                     /locus_tag="VIN75_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973931.1"
                     /translation="MPEPTFDSAPNDLPPPARDASLRPPRSTWDGGSDRSHVKTFVLA
                     ASLMGAIGLGALAVHEDVGALWRRAFGPPLTADEMRDRIGKCERDGDLP"
BASE COUNT         1906 a         4842 c         4732 g         1941 t
ORIGIN      
        1 gctttggcct gtccaccagg cccgcgagca cccgcccgcg cgagcagcca acatgtcgcg
       61 tctccagaga ctccagcgcg cgtccgcgca cgccgacatg gccgccgtcc gcgacaatgg
      121 cacctcgcca ggcggtaaga cctgacaccg aaaatttcaa gacctgagac cgcggccgtt
      181 cagcagtccg tcgcgctctc agcgcagctt caacgacaca cgccagccac cgtcgacggc
      241 ctccgtctcg aaccgcaccg gcaggaagcg ctcgatcatc tggaagttgg tcgtcgcgtg
      301 atcggtcagc tcggtgcacg tgtacgcggc cgcggtgccg cgcctgcaca cggccagcgc
      361 gagcggcagc gcccactggt cggccaggtg cgggcccagc gcggcggtcg agccgcggta
      421 gtcgcgcact tccttggcga gcgcctgcgc cacctgttcc gagctgacgc ccttctcgcc
      481 gaactgcgtg aacagctcgc acacgtgcgc gtgcgcgagc gtggccatca gcgcgttgcc
      541 cgggccctcg ttctggcgca cgacggggac gcgcagttgc gccggcgtcc agttcaacgc
      601 gcgcgccagc gcgtcgagct cgcgtcgcgc gaccgcgccc gggagcgcgg gcgcgaggca
      661 ctccgcgtag gactcgacgg cctcgccgcg ttcgagcagg tcgaacggct gcagcgtgcc
      721 ggcggccgga tgcaccgtca cgtcgatctc gccgccgccg gccgggtaga agccgtgccg
      781 gcgcagcgac agttcggact ccgccccgag ccgacgcagc agcggcgcgt agcagcgctg
      841 caggaaatgg aacggcggcg ccatcgggtt gtgggtgccg ccggcgagct tcaggcgcga
      901 cggcgcgtcg gccagcatca gcgcgggcca gacggtctgc agcaccagcg tgcagctgcc
      961 ggccgtgccg acgctgaacg cgtgctcgcc ggcgcgcacg gcgccgggct cgaacaccag
     1021 catgcccgag ccgagctcgg cgccctgcac cgtcgcgccg ctgaccgccg cggcggcctg
     1081 gacgcaggtc aggtgctggc gcatcaggcc tggcttggcg cggcgcgcgc ggatgcgcgc
     1141 gagcgtgaac ggctggccgg tgcacatgga cagcgccagc gacgtgcgca ggatctggcc
     1201 gccgccctcg ccgacggaac catcgatctc gatcatgggg accctcattc ttgttgtcag
     1261 ggccgcgcgt cgaagtggcg cacggcgtct tgcaggaact ggtcgagcgg cgcggtcggc
     1321 gccttgggct gccggtcgaa ggcatgcgcc tctgccacgg ccagctcggc ctcgatgaag
     1381 tcgtggatgc cgacccggcg cgggctggcg ccgattcgcc agcgcgcatc ttgacctccg
     1441 gcagcgcgtc gatctccgcg accagcgccg catcgagcga cgtcgccgcc accaggtccg
     1501 cgaagaccat gggcggcacc ccgcggcccg cgcggatcca gcgcgcggcc agcagcgggc
     1561 gcagcacgta caggtacttc ttgcagcgca ccgtctcgcc gaccaggagc tccctccgtt
     1621 cgaatcatcg tggcggccga ggaccaccgg ccctcagccc ttcacgcaca ccacctgctt
     1681 gagcgtgtgg acgatttcca ccaggtcgcg ctgggcggcc atcacggcgt cgatgtcctt
     1741 gtaggccatc gggatctcgt cgatcacgtc ggcgtccttg cggcactcga cgccttcggt
     1801 cgccttgatc tggtcgtcca ccgtgaacag cttcttggcc ttggtgcggc tcatcacgcg
     1861 gcccgcgcca tggctgcagc tgtcgaagct ctccgggttg ccgaggccgc gcacgatgta
     1921 gctgcgcgcg cccatgctgc ccgggatgat gcccatctgg cccttcctcg cgctcaccgc
     1981 gcccttgcgc gtgacgaaca cgtcctcgcc gaagtggcgt tcctgctgca cgtagttgtg
     2041 gtggcagttc accgcttcca cgtgcgactc gaacggcttg ggaatgacct tgcgcacgac
     2101 cgcgatcagg ttggccatca tcacctcgcg gttcctggcc gcgaacttct gggcccagct
     2161 cacgccgcgc acgtagtccc cgaagtactg cgcgccttcc tcgaagtacg ccaggtcctg
     2221 gtcgggcagg ttcgcgttgt tgcgcagcgc gtccttcttg gccagctcga tgaagtgcgt
     2281 gccgatcgcg ttgccgacgc cgcgcgaacc cgagtgcagc atgaaccaca cgaagcccgc
     2341 ctcgtccagg cagacctcga tgaagtggtt gcccgtaccc agcgtaccga ggtgcttgcg
     2401 gttgttcgtg ttcctcaggc gcgggtgcag ctcgcacagc gcgtcgaact cgcccgcgag
     2461 cgtcgcccag acctggtcga ccgtctcggg cgggttctcc cacgcgcctt cgtcgcgcgt
     2521 ccatcccttg gccgagcggc cgtgcggcac cgcgcgctcg atggccgagc gcagcgggcc
     2581 caggttgtcc ggcaggtcct gcgcgcgaag cgtggtcctg gcggccatca tgccgcagcc
     2641 gatgtccacg cccactgcgg ccgggatgat cgccttgagc gtcgggatca ccgagcccac
     2701 ggtcgcgccg atgcccaggt gcacgtcggg catcgcggcg atgtgcttga acacgacggg
     2761 caggcgcgcc gcgttggcga gctgcttctt cgcgtcgtcc tcgaccggga cgccgcgcgt
     2821 ccacatcttc accggcacgc cgccgggcac gtcttcgatg tcgtagttct gttccatgtt
     2881 cttcattctt ccccgaaaga gaggtcgcgc aacgaccagg gttggtgaaa cgtttcgcgc
     2941 tctatcccct gagctacggt cccgccgatt tgggaccggc cggactcgaa ccggcgacct
     3001 cgaccgttgc agggtgtagt tccacctgca ttcgttgcgt gacctcgaaa agatcgcgac
     3061 aagagtggat gggagtcagc gggattcgaa cccgcggtac cacgatgcag tcccatccgc
     3121 attcgcgaaa agcctgcctc cccgcgtggc gaccaacggt tggcgggaca tccttgtcga
     3181 tgtagtcccg ccggcattcg ccacgcgacg atgttccttc ctctactgcg cggccagcgg
     3241 gatcttgtcg atctcgccga cccagtgatc ggcgtccgcc cggccgcgag cgaagctcgc
     3301 gacgagatcg aacaccgcat ccgagaagcc gccgacattc aggatgtcgt gccgctcggc
     3361 cgcctgcgtc gtcgcgcccg gctgcaggtc gatgcacacg agccgcgcgg aggggttgcg
     3421 ctgcttcagc gcctcccact cgcgcagggt ctgcgtcgcg ccgcggcggt tcgcgtcgac
     3481 ccaggactcg ttgtccgaga ccaggatcac cacgtccacc tccgcgcgtt cgcgattcag
     3541 caaggccagc ggtgcactgc agttcgtgcc gccgcccccg atgctggcca gcgcctgcgc
     3601 gttggtcatc acggagtcgc ggccgttcaa cgacagcgac accacgccct gctcgaacgg
     3661 cagcacgcgc gccgacgggt tcttgcgcag caccgcagcg gccaccagcg ccgccacgtc
     3721 gatgcagcgc acgctgctcg tcgccgaacc gcggtggccc gtcacggcct ggctcatcga
     3781 gcccgacacg tcgggacaca ccaccacgcg gcccgcgaac gacggcacgt tggcgatcgc
     3841 cagctccatc gcatcctgca gcgcgtcgcg gatctccgcc ggaacgccct cgcccgtcgc
     3901 cttgaacgcc gccagcagct ggtacggcat cacgcgcgcc ttggcgatgg ccgcggggtc
     3961 gcgcagcctg gccgcgacca gctgcaccat gcggcgacgc tggaacacgc cgtgccgcgc
     4021 gaacgtgttc aggttctggc gcaccatctg ccacgagccg ttgcgcgcga cctgcgccca
     4081 ttggcccgtc gacagctcga gcgcggtcag catctggaac ggcacgtccg gcagctcgcc
     4141 gcccttgccc tcggcccgcg cccgcttgaa gcgttcgaag gcctgggtga gcggcggcag
     4201 cgccgacagc tccacgtcct tgccgatcac ccacgcgaac caggcggcgc gccaggcctc
     4261 atccggcttc ggatggacca tcttgaccac gtcggccagc gacggcgcgt tgcccacgca
     4321 ggcattcagc agctgccttt cgctcgcgtt cagcagccat tgctgcacca gcttcttggg
     4381 gcgcgagccc agcgacttgc gacccgtcgc gccgctgcgc aggatctgca cgaagtcgcg
     4441 cagcatcttg ccgttgtcga ccacgcggcc gaacacctgc gccagcagcg ccacgtcgcg
     4501 cacggccagc gtcgcggcca gcagcgcggg catgtccttc atgtggccgg cctggcgtgc
     4561 gtgcacggcc gtcttcgcga cgaaaccggg ttcgacctgc gcggccagcg ccagcaccgc
     4621 ctccagctgc gcctcggcgt cggcgtggaa cgtgtggccc aggcagccgg tggcggccag
     4681 ctgcgccagc tggtggcgcg gcgtgtacgc ataggccggg gccttcgacg cgttcgtcgc
     4741 ggtcggcgcc ggcagcaggg cgccgcgcag cgtctggaag agttgggtgt tgaccatgaa
     4801 gcgttgtcct tgcttgttca ttcaacgtgt cgtgcgctcc ttcttgcaac gcccatgcca
     4861 actcgtccgg cgcggcgcca ggccttcgca actcgttgat tcgattgagc attcgaacga
     4921 acgcacccga ccgagtgaaa cgtgcgctcc aggcggtttg cgtggatact agaattgcag
     4981 ctagcaaact agccatccat caatgaccaa gcgcaccgtc gtcatcggct tcctcggcac
     5041 gcagctcgat gccggccagg gcgcgggccg gtgggagaag tggcggccga ccctgtcgct
     5101 gatgcagcac gaggacacgg tgatcgaccg cctcgagttg ctgcacgcgt cggcgcacat
     5161 cggcctggcg cgccagctca aggccgacat cgccaccgtc tcgccagaga ccgccgtcaa
     5221 cctcgtcgcg ctcgacgtcg ccgacccatg ggacttcggc gagatgtacg cgggcctgta
     5281 cgactgggcg cgcggctacc gcttcgaccc ggcgcacgag cagtactggg cgcacatcac
     5341 caccggcacc cacgtcgcgc agatctgcat gttcctgatg gtggagtcgc gcttcatccc
     5401 gggcctgctg ctgcagacgg cgccaccgaa gaagcagcgc cagggcaatg ccggcagcta
     5461 cacgctgatc gacctggacc tgtcgcgcta cgacgcgctc gcgcgccgct tcgaccaggc
     5521 gcaggccgag gcgctggagt tcctcaagag cggcatcccc acgcgcaacg cgcgcttcaa
     5581 cgcgatgatc gaggagatcg agcgcgtggc cgtgcgctcg cgcgcgccca tcctgctgac
     5641 cggacccacc ggcgccggca agtcgcacct ggcgcgccgc atgtacgagc tcaagaaggc
     5701 gcgccaccag gtcaccggcg agttcgtgga ggtcaactgc gcgacgctgc acggcgacgg
     5761 cgcggcctcc acgctgttcg gccacaagaa gggcgcattc accggcgcgg tgaccgatcg
     5821 cgccggtctg ctgcgcagcg cgcacgaggg cgtgctgttc ctggacgaga tcggcgagct
     5881 gggcctggac gagcaggcga tgctgctcaa ggcgatcgag gagaagcgct tcctgccggt
     5941 gggcagcgat cgcgagacag gcagcgactt ccagctgatc gtcggcacca accgcgacct
     6001 gcgccgggac gtggccgccg gccgcttccg cgacgacctg ttcgcgcgca tcaacctgtg
     6061 gtcgtacgcg ctgcccgggc tggcgcagcg gcccgaggac ctcgagccca acgtcgactg
     6121 gctgctcgcc cgcgcggtgg ccgagtcggg ccacgcggtg cgcttcaacg tcgaggcgaa
     6181 gagccgctac ctgaagttcg cgcagtcgcc cggcgcggcc tggagcggca acttccgcga
     6241 cctgtcggcc agcgtcacgc gcctggccac cctggccgac ggcggccgca tctccaccgc
     6301 cctggtggac gcggagatcg cgcggctgca gtggctgtgg gagcgcaagc cggcggcgga
     6361 tgcggcggca gtcgacctcg ctgcgctgct ggatgccgcg acgctggccg acatcgacct
     6421 gttcgaccgc ctgcagctcg aggcggtgat ccgcacgtgc cgggagtcgg cgacgctgtc
     6481 ggacgccggc cgccgcctgt tcgaccgctc gcgcgagaag cgcagcgtcg tcaacgatgc
     6541 cgaccggctg cgcaagtacc tgctcaagtt cggcctggcc tgggaggccg tgcgcgcgtg
     6601 agcttcaggg cagcgtgtag tcgaagcggt acgaatgcgc cccgcccggc gcgatctcca
     6661 cgaccatgcg gcccgcgagt ccggccagcg aatcggtgcc ggagcccggc accacggtga
     6721 tcgactgcgt cggcacgccg tggtcgagcg tcgagctgtg ctgcagcgcg aacgagccgg
     6781 cgcggccgtc cagcgagccg cgcaccgact ccatcgcgac gtagccggcc gagccggcgc
     6841 ccgggttgcc gaacgacagc atctcgccgg tgctggtggc ctccagcgcg ccgtggaagc
     6901 gcttgtcgag cgacatgcgg gccaggccgc tgccgtcggc gacggcgctg agcggctgcg
     6961 gtgcgatctt cacgtcgaac gggccggtgg cggtatgcga ggtcatgggg tgatccttgt
     7021 cgaaggcggt cgccgcggcg caggcggcgg cgagcatggc ggcgatgagc aggtgcaggg
     7081 ggcgcatgat ggcatcatgg gttgcggccg gcatccggcc tcgcaagcat tgaacctcgc
     7141 tgcccatgac ggtgattgga aaaacgcgac ggccgctggg ccgggagccg cgcggcgtgc
     7201 tgcaccgcct cgcgctgggc gagttcgcgc acggccgctt cgaggcgcct gacgacctgg
     7261 ccgaccgcat cgagcatttc tggacggtgc gctggaacct cgacggcctg ccgccgcagg
     7321 tgcaggagac gttgccgcat ccgaacgtgc acctggtcat cgagcccggc agcatcgcgg
     7381 cctggggcgt gcacacggga cgctggacgc gcgtgctcga gggccgctcg caggccttcg
     7441 gcgtcaagtt ccggcccggg gcgttccgtg ccctgctggg tcgcgcggtc tcgacgatcg
     7501 ccgacggctc gatcgacgcg cgggcgatct tcggcgccga cgccgcggcg ctggccggcg
     7561 tgctgggcca cgacgatgac cgcgacgccg cggccctcgc gtcggccttc ctgcgcgagc
     7621 gcctcgcacc ggcgacggac gacgccctgc tcgcgggccg catcgtcgac agcgtcgccg
     7681 acgacatcga gctgcactcg gccgacgagc tcgcgcgccg cttcggcatg agcctgcgcg
     7741 cgctgcagcg tctgttcaac cagttcgtgg gcgtcgggcc gaagtgggtg atcaagcgct
     7801 accggatgca cgaggccgtg gcgcgcgtgc aggccgggca gccggtgtcg tgggccgcgc
     7861 tggcgcagga cctcggctac ttcgaccagg cccacttcat cgcggacttc cgcaagctgg
     7921 tgggcatgac gcccggcgac tatgcgcgcg agcggcaccc gcgctgaggc tgctcagagc
     7981 gccgcgcggc gcgcccgcag gctctcgcgc acgaccgcga tgtggctgcg caggccgtac
     8041 agcaggtcgc cgaatgccgg cggcacggcc aggcgcgtga ccgtcgtctc gacgtgctcc
     8101 agccgcgcga gcagcgcgtc gcagcgctcg gcgtccagcg gcccggcgtc ggcgtcgcgc
     8161 tcgatgcgct gcagcaccgc gtaccagcgg tagatgcgcg agacgacgcg ccagcggaac
     8221 acggccggcg cgatcttgaa gcccggcacc aggatgatgg tcatcggcac cacgatcgcc
     8281 agcacgcgct cgatcaggct cgcgatccag aacgggaacg agcggtacag gaagctgcgg
     8341 cccgaggcgt agaagcgcga ggcgtcgccg ctgatgggga agtcgtgttc gatcgcggac
     8401 gggaaatcgc ccgccttctt gtacaggccg gcgcggccgt gcacctcgcg cgcggcggtc
     8461 agcagcaggt ccgacagcgc cgggtgcagg tcgtcgcgcg cgatgagttc gacggtcggc
     8521 gcgatcagcg tcacgctctc gggcgggatg tcctcgccga agtcgatcgc cccgcgcggc
     8581 agcaccagct tgttcaggta agtgatgcgg tgggtgtagc cctcggcctg cacgaagttg
     8641 aacaggtgca cgtcctcgtc gcgcagcagc tcgcgcatca gcgccgtggg cgtggagtcg
     8701 ctcatggcga acagcgcgtc gatcttgccc tccttgagca gctggatcga gtcggcgggc
     8761 gggcggtggc cgacctgcgt gccgtcgccg ggcttgatgc cgttggcgtc gagcagcgtc
     8821 tgcgccagcg cggacgcgcc gctgccttcc aggccgatgt cgagccggtg ctgcttgaaa
     8881 tcggagatca gcgtcctggg cttgccgcga tagaagatca tcatcggctg gtatgacagg
     8941 ctgcccagcg agaccaggtg cgaggtgtcg tggccggtcg tctcgccgcc gacgacgaag
     9001 ccgacgtcca cgtcctgctt gccgtcctcc agccgcgcga ggttgtcgcg cgaaccctgc
     9061 gaggtcagga tcttgagcgt cacgccctcg cgcgccagga tcttgcggta ctgctcggcg
     9121 acattcgcga acgagctgcc cttggggccg ctggtcagca ccagcgtggt gggcggcgcc
     9181 gagcgcagga acacgaacgc gacgatggcc gcgatcaggc tcaggagcag cagcaggccc
     9241 aggctgatcg gcagcccgcg gccgacgacg cccagggcgt cgcgcagctg cgcgaggcgc
     9301 gcacgcgacg cgtcgacgag cgacggatcg tcgcctcgct gcgggggaag tgaagacatc
     9361 gcggatccgg gcgccaggag atgccgggat gatagacgcg ggcggtggcg gatgtgcgcc
     9421 gttctggggc ggaggcgtca cgggaggtgt ccggcctgcc gggaagggcc cggcattgcc
     9481 ccggtcgcgc aaggcattgc ggctcgcggt gaccgagcgg cgcgatcgag gcaatgccgg
     9541 accgctctcg tggcggcgcc tcgacgcgca gcagatgcac cgggctcacc ccagccgcat
     9601 cgatacctcc acatccccgc cgaacggcgt cgacaggtac ccgccgtccg ccgaccggac
     9661 gcgcgccagg tactcggggc tgtcatcggc gttgtccgcg acgaccagcg ccccgggccg
     9721 caggcgcggc tccagcagcg cgaggatctc gggatacagc gccttggcgc catcgagcag
     9781 caccaggtcg atggcgccgg gcaggtcgcg cgccagcgtc tgcagcgcat ccccttcgcg
     9841 gatctcgacg aggtcgtcca ggccggccgc ggcgaggttc gcccgcgcgc ggaccacctt
     9901 ggacggctcg aactcgcacg tgatgaggcg cccgccgctg ccgttgtcgc gcagggcggc
     9961 ggccaggtgc agcgtcgaca ggccgaacga ggtgccgaat tcgacgatcg cgtgcgcgcc
    10021 ggtgctgcgc gccagcatgt acagcagcgc gcccgtggcg cgcgagacgg gcagcggctg
    10081 gtccttcagg cgcgtgtaga agtcgaggta ctcggtcctg ctgtgcatca gccgcgcgag
    10141 ctcgccgggc ggcagcgcgg cggccatcgc gttcaggccg atcgcggcct cgtcggcctc
    10201 ggcgaacagg cgcgccagca gcggcgcgac gggagcggtg gtgagcgtgt cggtcatggg
    10261 tcgggtttct cgtggaggaa tggtgtcgcc ataatatgaa caatccttcg cgtttcccat
    10321 tcgcattgcg agtgccgccc atgaccgacc gcaccacctc gcgcctgtcc tcgcgcaaga
    10381 ggccccggca ggcgcgctcc accgacctcg tcgcggcgat cctggaggcg gccattcagg
    10441 ttttgtcccg ggaaggcgcg cagcgcttca ccaccacgcg cgtggccgaa cgcgccggcg
    10501 tcagcgtcgg ctcggtctac cagtacttcc ccaacaaggc agccatcctg ttccggctgc
    10561 agagcgacga gtggcgcgag acgaccgacc tgctgcgtgc cacgctcgcc gacaccagcc
    10621 gtccgccact cgagcgcctg cgccggctgg tgcatgcgtt cgtgaagtcc gagtgcgacg
    10681 aggccgcgat gcgcgtcgcg ctcgccgacg ccgcgccgct gtaccgcgac gcgcccgagg
    10741 cgcaggaggt gcacgccgcg agcaagggcc tgctgcacgc gttcatgcgc gagacgctgc
    10801 ccgatgcgaa gccggccacg cgcgcccgcg cgggccggct gatcaaggcg acgctgagcg
    10861 ccgtcgggca cgacttctcg gggtcgccgc gcagcgcgcg cgagatcgcc gcgtactccg
    10921 acgcgatggc cgacatgttc gcggcgtacc tcgtcgcgct gggccacctc gagccggtgc
    10981 ctgcgcgcgg gcgggcgcgt ctcgcgcgcg cggccgcctg acctgatcag ccctgcacct
    11041 cgacgacgac cttgccgcgc gcgcggccgg ccgccacttc ctcgtgcgcc tcgagcgccg
    11101 tgtccagcgt gtagcgccgc gcgtccatcc gcggcacgag gcggccgctc tcgaccagct
    11161 tggcgacctc ctccagtatc tcgccatggt gcgcgcggcg ctcgcccgtg agcatcggca
    11221 gcagcgtgaa cacgcccgag tacgaggccg cgcggaacga cagcggcgcg agcgagtgcg
    11281 tgccccagcc cagcgcgctg acgacgtggc cgccgtacgt gcgcgccgcg cggaaggagg
    11341 cgtcgagcac ggcaccgccg acggtgtcga acacgacgtc gaagccccgg ccggcggtgt
    11401 gctcgccgac gtagtcgtcg accgtggccg ccacgtagtc gatcggcgtc gcgccgaagc
    11461 ggcgcacgat gtcggcgtcg gcggccgacg cggtggcgaa cacctgcgcg cccaaggccc
    11521 tggcgatctg caccgccgcg tggcccacgc caccggcacc gccgtgcacc agcaccttct
    11581 ggccggcctg cacgccggcg cggtcgacga ggccttccca cgcggtgatc gcgatcaatg
    11641 gcagcgccgc ggcctcgcgc atcgacaggt tggcgggctt gcgcgccagc aggtcggcgt
    11701 cgacggcgac gtattcggcc agcgagccct gcaggccggc gacgccgccc gtcaggccgt
    11761 agacctcgtc gccgacgcgc cagcgctcga cgccggcgcc gacgccgcgc acgacgccgg
    11821 ccaggtccat gcccagcacg ccgggcagcg gatgcctcgc atgcgctgca gcgccggcgc
    11881 ggatcttgga gtccagcggg ttgacgccgc tggcatgcac ctgcaccagc acctggccgg
    11941 gtccggcgac gggccgcgcg acctggcgcg agacgaaggg cgtgttggcg gcctcgagga
    12001 cgagggcgcg catcgaagcg gtgtcggcgg tcatggcggt tccttgtgtt ggggtgacgc
    12061 cagtgttcgc cattcatgat tgcatgtaaa ttgacgatcc tgcacgactt ccattcactt
    12121 ctgaatacct cgccatggaa tggagcgaca tccggcactt cctcgcgatc gcccgcgcgc
    12181 gctcgctcaa cggcgcggcg cgcctgacgg gccagagcca gccgacgatg ggccggcgcc
    12241 tgcgcgcact ggagcatgcg ctgggccatg cgctgttcca gcgcggcagc gagggcttcg
    12301 tgctcaccga cgagggcgcg gcgctgctcg cgcacgccga gcggatggag gaggaggcgc
    12361 tcgccttcga gcgcgagctc gcgggccagg gcgaggagct cgacggccag ctgcgcgtgt
    12421 cgtcgtccga ctggttcggc gtgcacatgc tggccccggt cctggccgaa ttcatcaagg
    12481 cgaacccgcg cgtggccatc gagctggtga ccgacgcgcg gctgctgaac ctggcgcgtc
    12541 gcgaggccga cctcgcgttc cgcatccagg ccttcgccga gccggatgtc atccagcgcc
    12601 gcctgatgac catcccctac gggctgtacg ccgcccaggg ccgcgcgcgt gcgctggcca
    12661 aggacggctc cggctgcgcg ctgatcacgc tggacgccgc ctaccgagac ttccccgacg
    12721 cgcgctggct gcgccaggtg ctgcccaagg cgcacgtggc cttcaccagc aacagccgcg
    12781 aggcgcaggc tcgcatgtgc gccgagggcg tcgggctggc cgtgctgccg cggccgctcg
    12841 gcgacggcat ggcgggactg cgcctggtca ggacggacgc cccgccgccg ggccgcgacg
    12901 tgtgggtcgg cttccatcgc gacctgcgcc ggctgcggcg gctgcgcgcg ctggtcgacc
    12961 tgtgcgtgga gcggctggcg gcctgagcgc gcggcgcggg caatccgtca cgcggcgcgc
    13021 gggcaaccgc cgagtgcgcg gggcccgcgc cggcgcccgc cgcgtcgata atccgacgcc
    13081 aagggagcga ggcatcgcgc gcggcgcgag gcgccggttc gcccctggag cctgccccgc
    13141 cgatgcccga acccaccttc gactccgcac cgaacgacct gcccccgccc gcccgcgatg
    13201 cgtcgctgcg tccgccgcgc tcgacctggg acggcggcag cgaccgctcg cacgtgaaga
    13261 ccttcgtgct ggcggccagc ctgatgggcg cgatcggact gggggcgctg gccgtccacg
    13321 aggacgtcgg cgccctgtgg cggcgcgcgt tcgggccgcc gctgaccgcc gacgagatgc
    13381 gcgaccgcat cggcaagtgc gagcgcgacg gcgacctgcc c
//
LOCUS       DATCRO010000254         4878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_768194, whole genome shotgun sequence.
ACCESSION   DATCRO010000254 DATCRO010000000
VERSION     DATCRO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768194"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VIN75_06925"
     CDS             complement(<1..240)
                     /locus_tag="VIN75_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973932.1"
                     /translation="MNAGHAGSSGASAVHASSVLVVDDEPDLLTLYELTLLREGLEVE
                     TAATVAQAREILQTRLFRAVISDMRLPDGTGLDLLQ"
     gene            complement(249..2084)
                     /locus_tag="VIN75_06930"
     CDS             complement(249..2084)
                     /locus_tag="VIN75_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973933.1"
                     /translation="MWGSKERKARRAAKSREGADAGLQEESWFNTMDGSGEPSLPAPG
                     AGGAWNTPVLRPLAGHALRRIFGTYVAARAALGIALVLANALMGMATGRFVSAPLLLC
                     GAYAVQALVWWLTDEFRMFRVLQSARWQWWMTIGVDLVIFGAIHWIDPTSPLNFVALL
                     VLPVLMAGILTPRLPALATAAGVALMLLAAAGRVYGTVPDSPALMSQAGLSGIGLFVV
                     ALLAGELSMRLAHEERTARDSRERARQQAQLSRLVIEDMADGVLVIDRQLRVRAANPS
                     ARRLLVAQAARCPSPPFSLRLHPGWLVLADEVEQAFARGTWPAAPFDLNILLEDSTRR
                     ALHIRARFTRSRTDGNDTTPADEPLCVLFIEELRAVQARLRQERLAAMGRISAGVAHE
                     IRNPLAAIAQANALLEEDELRPDQRRLIRIMTDNIDRLRRIVDDVMEAAPGSLAPSRT
                     LDASAEIASICSEWARTVGLALGSASRLQVLLPSTPVGVVFDPDHLRRVLVNLLDNAI
                     RHASAAPGGVRVTMTTSDDGAWASLAVASDGEPIPLDVEPYLFEPFHSTRSCGTGLGL
                     YICRELCERHGARIDFRRHNERHRNVFEVMMPRESLTSAEGRLVL"
     gene            complement(2114..2359)
                     /locus_tag="VIN75_06935"
     CDS             complement(2114..2359)
                     /locus_tag="VIN75_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY8973934.1"
                     /translation="MVGRLLVWLILGFLAWWMLRPRKAAPPPAAGRTPPAAGAAPEAM
                     VDCAHCGLHFPASEAVRDGARSYCCSAHRDAGPSRAQ"
     gene            complement(2361..3161)
                     /gene="ccsA"
                     /locus_tag="VIN75_06940"
     CDS             complement(2361..3161)
                     /gene="ccsA"
                     /locus_tag="VIN75_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8973935.1"
                     /translation="MILSSGLIHALAWAAAAAYALSGLGRAAWGARAQVAALVLHGLW
                     IASTFVGEADAGLGIRWGFAPVLSLTAWLVLAVHFTESRLVPISGLRRIFALIGAASV
                     LLALAFPGDIVIVSYSVYAPLHWTLGIAAYALFGVAVVHAVMLDRAERQLRQKAGAGL
                     LGMPLLSLERLTFLFVEAGFVVLTLAIVIGLFTMAHWRWGDHKTVLSLASWATFAALI
                     VGRRTRGWRGRQATRWVYAGAVLMLLAYAGSRFVFEVVLQRAPVSLGN"
     gene            3216..4589
                     /gene="ffh"
                     /locus_tag="VIN75_06945"
     CDS             3216..4589
                     /gene="ffh"
                     /locus_tag="VIN75_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8973936.1"
                     /translation="MASTLTDRLSRLVKTMRGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKALGQEVVASLTPGQVLVSIVHKELAATMGEGVADINLAVQPPAV
                     ILMAGLQGAGKTTTTAKLAKYLIDKRKKKVLTVSGDVYRPAAIEQLKTVTRQAGAEWF
                     PSTPDQKPHDIAAAAIDYARKHYFDVLLVDTAGRLAIDELLMAEIKDLHALLRPVETL
                     FVVDAMQGQDAINTAKAFREALPLTGIVLTKLDGDSRGGAALSVRQITGAPIKFAGVS
                     EKIDGLEVFDADRHAGRVLGMGDIVALVEGVTKGLDVEAAQKLAEKVRSGNDFDLNDF
                     LAQLSQMKKMGGLAGLMDKLPTEMTAKAGQPDMDKAERDMRRMEGIINSMTPLERRKP
                     ELLKAKRKFRVAKGAGVQVQDVNRLLNQYEQMRDMMKKMRGGGLMKMMKKMGAMKGMM
                     GGGMPKL"
BASE COUNT          802 a         1782 c         1691 g          603 t
ORIGIN      
        1 ctgcagcagg tccaggccgg tgccgtcggg cagccgcatg tcgctgatga cagcgcggaa
       61 caggcgcgtc tgcaggatct cgcgcgcctg ggcgacagtg gccgcggtct cgacctccag
      121 gccctcgcgc agcagcgtca gttcgtacag cgtcagcagg tccggctcgt cgtcgaccac
      181 cagcacgctg ctcgcgtgca cggcgctcgc gcccgacgag ccggcgtggc cggcattcac
      241 tgcgcttgtc ataggaccag tcggccttcc gccgatgtca gcgattcccg cggcatcatc
      301 acctcgaaca cgttgcggtg ccgttcgttg tggcgccgga aatcgatccg ggcgccgtgg
      361 cgttcgcaca gctcccggca aatgtacaac ccgaggcctg tcccacagct gcgtgtggaa
      421 tggaagggct cgaacaggta gggctcgacg tccagcggga tcggctcgcc gtcgctggcc
      481 accgcgagcg acgcccaggc gccgtcgtcg ctggtggtca tcgtcacgcg cacgccgccg
      541 ggcgcggcgc tcgcgtggcg gatcgcgttg tccagcaggt tgacgagcac gcggcgcagg
      601 tggtcggggt cgaacacgac gcccaccggc gtggacggca gcagcacctg cagccggctc
      661 gcgctgccca gcgccaggcc gacggtgcgc gcccattcgc tgcagatgct ggcgatctcg
      721 gcgctcgcgt cgagcgtgcg cgacggcgcg agcgaacctg gcgccgcctc catgacgtcg
      781 tcgacgatgc ggcgcagccg atcgatgttg tccgtcatga tgcggatcag ccggcgctgg
      841 tcggggcgca gctcgtcctc ctccagcagc gcgttggcct gcgcgatggc ggccagcggg
      901 ttgcggatct cgtgggcgac gccggcggag atgcggccca tcgccgccag ccgctcctgg
      961 cgcaggcgcg cctgcacggc gcgcagctcc tcgatgaaca gcacgcacag cggctcgtcg
     1021 gcgggggtgg tgtcgttgcc gtcggtgcgg ctgcgcgtga agcgcgcgcg gatatgcagc
     1081 gcgcggcgcg tgctgtcctc cagcaggatg ttcaggtcga acggcgccgc cggccaggtg
     1141 ccgcgcgcga acgcctgctc cacctcgtcg gccagcacca gccagcccgg atgcagccgc
     1201 agcgagaacg gcggcgacgg gcagcgcgcg gcctgcgcca ccagcagccg gcgcgccgac
     1261 gggttggcgg cccgcacgcg cagctggcgg tcgatcacca gcacgccgtc ggccatgtcc
     1321 tcgatcacca gccggctcag ctgggcctgc tggcgcgcgc gctcgcggct gtcgcgcgcg
     1381 gtgcgctcct cgtgcgcgag gcgcatcgag agctcgcccg ccagcagcgc caccacgaac
     1441 aggccgatgc cgcttaggcc cgcctgcgac atcagcgccg gcgagtccgg caccgtgccg
     1501 tacacgcgcc ccgccgccgc cagcagcatc aacgcgacgc cggcggccgt cgccagcgcg
     1561 ggcaggcgcg gcgtcaggat gccggccatg agcaccggca gcaccagcag cgcgacgaag
     1621 ttcagcggcg acgtcggatc gatccagtgg atggcgccga agatgaccag gtcgacgccg
     1681 atcgtcatcc accactgcca gcgcgccgac tgcagcacgc ggaacatgcg gaactcgtcg
     1741 gtgagccacc agaccagcgc ctgcacggcg taggcgccgc acagcagcag cggcgcgctg
     1801 acgaatcggc cggtggccat gcccatcagc gcattggcca gcaccagcgc gatgcccagc
     1861 gccgcgcgcg ccgccacgta ggtgccgaag atgcgtcgca gcgcgtggcc cgcgagcggc
     1921 cgcagcaccg gggtgttcca tgcgccgccc gcgcccggcg ccggaaggga cggctcgccc
     1981 gagccgtcca tcgtgttgaa ccacgactcc tcctgcaggc cggcgtcggc gccctcgcgc
     2041 gacttggccg cgcgtcgggc cttgcgttcc ttgctacccc acatggacgg ccacgcgcgg
     2101 attcacgacg cgatcactgc gcacggctcg gccccgcgtc gcgatgggcg ctgcagcagt
     2161 acgaccgggc gccgtcacgc acggcctcgg acgccgggaa gtgcaggccg cagtgcgcgc
     2221 agtcgaccat cgcctcgggc gcggcgcccg cggccggggg ggtgcgcccg gcggcaggcg
     2281 gcggcgcagc cttgcgcggg cgcagcatcc accaggcgag aaagcccagg atcagccaga
     2341 caaggagccg tccgaccatg tcagttgccc agcgagaccg gcgcgcgctg cagcacgacc
     2401 tcgaacacga agcgcgagcc cgcgtaggcc agcagcatca gcaccgcgcc ggcgtacacc
     2461 cagcgcgtgg cctggcggcc gcgccagccg cgcgtgcggc ggccgacgat cagcgccgcg
     2521 aacgtcgccc agctggccag cgacagcacc gtcttgtggt cgccccagcg ccagtgcgcc
     2581 atcgtgaaca gcccgatgac gatggccagc gtcagcacga cgaagccggc ctcgacgaac
     2641 aggaacgtga gccgctcgag cgacagcagc ggcatgccca gcaacccggc gccggccttc
     2701 tggcgcagtt ggcgctccgc gcgatcgagc atcaccgcat gcaccacggc cacgccgaac
     2761 agtgcgtagg cggcgatgcc cagcgtccag tgcagcgggg cgtagaccga gtacgagacg
     2821 atcacgatgt cgcccgggaa cgccagcgcc agcagcacgc tggccgcgcc gatcagcgcg
     2881 aagatgcggc gcaggccgga gatcggcacc aggcggctct cggtgaaatg caccgccagc
     2941 accagccagg ccgtcagcga cagcaccggg gcgaagcccc agcggatacc cagccccgca
     3001 tcggcctcgc cgacgaaggt gctggcgatc cacaagccgt gcaggaccag ggccgccacc
     3061 tgcgcgcggg cgccccaggc ggcgcgtccc agccccgaca gcgcgtaggc cgccgcggcg
     3121 gcccacgcca gcgcgtggat gaggccagac gatagaatca tccccgcagt ttactaaggc
     3181 gcgcgccact catccttgcg tgcgctcacc ccccgatggc ctccaccctc accgaccgcc
     3241 tgtcgcgcct cgtcaagacg atgcgcggcc aggcccgcat caccgaatcg aacgtccagg
     3301 acatgctgcg cgaagtgcgc atggccttgc tcgaagccga cgtggcgctg cccgtcgtgc
     3361 gtgacttcat cgcgcgggtc aaggacaagg cgctcggcca ggaagtcgtc gcctcgctga
     3421 cgccgggaca ggtgctcgtc agcatcgtgc acaaggaact cgccgcgacg atgggcgagg
     3481 gcgtggccga catcaacctg gccgtgcagc cgcccgccgt catcctgatg gccggcctgc
     3541 agggcgccgg caagaccacc accaccgcca agctcgccaa gtacctgatc gacaagcgca
     3601 agaagaaggt gctgacggtc tcgggcgacg tctaccgccc ggccgccatc gagcagctca
     3661 agacggtcac gaggcaggcc ggcgcggagt ggttcccgtc cacgcccgac cagaagccgc
     3721 acgacatcgc cgccgccgcc atcgactacg cgcgcaagca ctacttcgac gtgctgctgg
     3781 tcgacaccgc cggccgcctg gccatcgacg aactactgat ggccgagatc aaggacctgc
     3841 acgcgttgct gcgtccggtc gagacgctgt tcgtcgtcga cgcgatgcag ggccaggacg
     3901 cgatcaacac cgccaaggca ttcagggaag cgctgccgct gaccggcatc gtgctgacca
     3961 agctcgatgg cgactcccgc ggcggcgcgg cgctgtcggt gcggcagatc acgggcgcgc
     4021 cgatcaagtt cgcgggcgtg tccgagaaga tcgacggcct ggaggtgttc gacgccgacc
     4081 gccacgccgg ccgcgtgctg ggcatgggcg acatcgtcgc gctggtcgag ggcgtcacca
     4141 agggcctgga cgtcgaggcc gcgcagaagc tcgccgagaa ggtgcgctcg ggcaacgact
     4201 tcgacctgaa cgacttcctg gcccagctga gccagatgaa gaagatgggc ggcctcgccg
     4261 gcctgatgga caagctgccc accgagatga ccgccaaggc cggccagccc gacatggaca
     4321 aggccgagcg cgacatgcgc cgcatggaag gcatcatcaa ctcgatgacg ccgctggagc
     4381 gccgcaagcc cgagctgctc aaggccaagc gcaagttccg cgtcgccaag ggtgccggcg
     4441 tgcaggtgca ggacgtcaac cgcctgctca accagtacga gcagatgcgc gacatgatga
     4501 agaagatgcg cggcggcggc ctgatgaaga tgatgaagaa gatgggcgcc atgaagggca
     4561 tgatgggcgg cggcatgccc aagctctgac cggtcctcgg gcccaaacgc attcccgtct
     4621 ggcgcgctcc ggttcaatac ccgcgccgct gtcccgccgc cagcacgatt cagcccggcg
     4681 ggctaggatc ccggcttcat tcgaacgacc tcgttggaga agagccatgc ttcgtcgcct
     4741 gaccccgctg ctggcagcgg ccactcttgc cgccttcggc acgtccgccc acgcggccct
     4801 gaagccgggc gacaccgccc cgctgttcac gaccgaggcc gcggtcggcg gcaagcagat
     4861 gccgctggtg ctggccga
//
LOCUS       DATCRO010000255         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_239046, whole genome shotgun sequence.
ACCESSION   DATCRO010000255 DATCRO010000000
VERSION     DATCRO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239046"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VIN75_06950"
     CDS             complement(<1..1521)
                     /locus_tag="VIN75_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8973937.1"
                     /translation="MRAGLLAVDQLVAALAADHAPVVLDVRDLVESEAGHLPGAVIVP
                     RRRLEFRIDALVRDRATRLVVVDSGLEIAPGVLDPRAALAADTLLAFGYREVAALDGG
                     VAAWRQAGLPTVSGTQVSSRAFGRRVADLERVPLIDVATLRQWQQSGRRVALCDVRSA
                     AEHTQDCVPGAVSLPGFEAVSHALDMAADSDVIVLCSSARTRSVAVARTLIDLGLSDV
                     VALDGGMLAWRLAGLAPEQGSRRRRVQPSNASRQFAELGSARLAKHHGVERVEAAELA
                     ALVQADGNFHAFDLRNLGQHGVAHVPRSVPVACDMLLVRRDELIAADGAPVVLVDDDA
                     VRAHLTGVWLRRLGHARVHTLAGGFAAWINGGRPARTTAEMPLGWREASSATPGLGVD
                     DIARWLAAWAPARILHVDTSASWQRGHLPGALWLPRGWLEARIADVAPSADEALLVTC
                     NDGAQSAYAAATLRQRGYSNVAWLQGGTRAWAAAGRGLEAASTPQPGDDALLPPARR"
     gene            complement(1868..>2514)
                     /locus_tag="VIN75_06955"
     CDS             complement(1868..>2514)
                     /locus_tag="VIN75_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8973938.1"
                     /translation="AMLGGMNLCIYHAFGRVPIGIATAIETTGPLVVVLAASRRALDV
                     AWVALAIAGLALLLPLSSDRALDPLGLLFSFGAALWWALYIVFGKRVSTLASGQAVSL
                     GMLVAALLAVPLGVADAGAAMVQPAPLLAGLGVALLSSAVPYSLEMAALRRLPRHVFG
                     ILVSATPAVGAACGAIVLGERLSAPQWLAIALIIGASAGGAASAGRPSPGTAGV"
BASE COUNT          356 a          974 c          935 g          249 t
ORIGIN      
        1 gcggcgtgcc ggcggcagca gggcgtcgtc gcccggttgc ggggtcgacg ccgcctcgag
       61 cccgcggccg gcggcggccc aggcgcgcgt gccgccctgc agccacgcga cgttcgagta
      121 gccgcgctgg cgcaacgtgg ccgcggcgta ggccgactgc gcgccgtcgt tgcacgtgac
      181 cagcagcgcc tcgtcggccg acggcgcgac atcggcgatg cgcgcctcca gccagccgcg
      241 cggcagccac agcgcgccgg gcaggtggcc gcgctgccat gacgcgctcg tgtccacgtg
      301 caggatgcgc gccggcgccc aggccgccag ccagcgcgcg atgtcgtcca cgcccaggcc
      361 gggcgtcgcg ctgctcgcct cgcgccagcc cagcggcatc tcggccgtcg tacgcgccgg
      421 gcggccgccg ttgatccatg ccgcgaagcc gccggcgagc gtgtgaacgc gcgcatgccc
      481 cagccggcgc agccagacgc ccgtgaggtg cgctcgcacg gcgtcgtcgt cgacgagcac
      541 gacgggcgcg ccgtcggccg cgatcaactc gtcgcgccgc accagcagca tgtcgcaggc
      601 caccgggacg ctgcgcggca cgtgcgccac gccatgctgg cccaggttgc gcaggtcgaa
      661 cgcgtggaag ttgccgtcag cctgcaccag cgcggcgagc tccgcggcct cgacccgttc
      721 gacgccgtgg tgcttggcca gccgcgcgga gcccagttcg gcgaactggc ggctcgcgtt
      781 cgagggctgg acgcggcggc gccgcgagcc ctgttccggc gcgaggcccg ccagccgcca
      841 ggccagcatg ccgccgtcga gcgccacgac gtcgctgagc ccgaggtcga tcagcgtgcg
      901 cgcgacggcc acgctgcgcg tgcgcgcgct cgagcacagc acgatgacgt cgctgtcggc
      961 ggccatgtcg agcgcatgcg acacggcctc gaagccgggc agcgagacgg cgcccgggac
     1021 gcaatcctgc gtatgttcgg cggcgctgcg cacatcgcac agcgccacgc ggcgccccga
     1081 ctgctgccac tggcgcagcg tcgcgacgtc gatcagaggc acgcgctcga gatcggcgac
     1141 gcggcggccg aaggcccggc tcgacacctg cgtgccgctg accgtcggca gccccgcctg
     1201 gcgccacgcg gccacgccgc cgtccagggc cgcgacctcg cggtagccga acgccagcag
     1261 cgtgtcggcg gccagcgcag cgcgcgggtc gagcacgccc ggcgcgatct ccaggccgct
     1321 gtcgacgacc accagccgcg tggcgcgatc gcgcacgagc gcgtcgatgc ggaactcgag
     1381 gcggcgccgc ggcacgatca ccgcgccggg caggtggccg gcctcggact cgacgaggtc
     1441 gcgcacgtcc agcaccaccg gcgcatgatc cgcggccagc gcggcgacga gctggtcgac
     1501 ggcgagcagg ccggccctca tgggcgggcc ctggcagggg atcgccgcgt cgcgccccac
     1561 gggactcggg ccaggccgga cggccgcggc ggactcatgg ccggcgctct cgaaacgcgg
     1621 ggcgcacagg ggcgcgacgt gtgcgcggga ttcgcgacac gtccggatgg gcggcgtccc
     1681 gggcgggacg gccagcaacg agacgacgcg atctgaccat gcgcgaacct cgggacctcg
     1741 gtggcggccc gcggagtcat cacgcgatga gcgcaggccg tcgaaccagt ctaggagtcg
     1801 cttccggtgg ggtcaatcgc tgccgggtat cgagatccgg gccctgacag catgcgacac
     1861 gctttcccta cacgccggcc gtgccgggcg acggccggcc ggcgctcgcc gcgccgcccg
     1921 ccgacgcgcc gatgatcagc gcgatggcca gccactgcgg cgccgacagc cgctcgccca
     1981 gcacgatcgc gccgcacgcc gcgccgaccg ccggcgtcgc gctcacgagg atgccgaaca
     2041 cgtggcgcgg caggcggcgc agcgcggcca tctccagcga atacggcacc gcgctggaca
     2101 gcagcgccac gccgagcccg gccagcagcg gggccggctg caccatcgcg gcgcccgcat
     2161 cggccacgcc cagcggcacc gccagcagcg ccgccaccag catgcccagc gacacggcct
     2221 gcccgctcgc gagcgtggac acgcgcttgc cgaacacgat gtacagcgcc caccacagcg
     2281 cggcgccgaa cgagaacagc aggcccagcg ggtcgagcgc gcgatcgctc gaaagcggaa
     2341 gcagaagcgc caggcccgcg atggccagcg cgacccacgc gacgtcgagc gcgcggcgcg
     2401 acgcggccag cacgacgacc agcggcccgg tggtctcgat ggcggtggcg atgccgatcg
     2461 gcacgcggcc gaacgcgtgg tagatgcaca ggttcatgcc gcccagcatg gcgc
//
LOCUS       DATCRO010000256         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_813045, whole genome shotgun sequence.
ACCESSION   DATCRO010000256 DATCRO010000000
VERSION     DATCRO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813045"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..460
                     /locus_tag="VIN75_06960"
     CDS             38..460
                     /locus_tag="VIN75_06960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8973939.1"
                     /translation="MSIPTAAELNRMGADCLPGHLGVAITHVDAGELRAELAVSMKLM
                     APNGYLHAGSVVTLADTSCGYACIANLPEGATGFTTIEQKSNFLGTAREGTLDCVATA
                     QHRGRTTQVWDAVVTHRETGRTIALFRCTQMILWPQSA"
     gene            503..880
                     /locus_tag="VIN75_06965"
     CDS             503..880
                     /locus_tag="VIN75_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY8973940.1"
                     /translation="MTDSSTPVRRLVEIRAYRLKPGTRADFHVAASELALPMVRAYGM
                     DVVAHGPVPNDPNGYFLVRSFASLAQLTAQEDEFYGSVRWREGPREALVSRIETYVDT
                     LLWLSPAAIDDLRAGNVAPAVVP"
     gene            complement(1013..1189)
                     /locus_tag="VIN75_06970"
     CDS             complement(1013..1189)
                     /locus_tag="VIN75_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973941.1"
                     /translation="MAKPNYAYEKRQRELEKKQKKEDKALKKAAGSHAGAQDDQAPAE
                     AQAPAAAVADKSAD"
     gene            complement(1347..1553)
                     /locus_tag="VIN75_06975"
     CDS             complement(1347..1553)
                     /locus_tag="VIN75_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEY8973942.1"
                     /translation="MDQAIVGYHLDDEGHWVAELACGHFQHVRHDPPWQNRPWTQTPE
                     GRAAMRGRTLACRKCDQGAPRDSI"
     gene            complement(1558..1938)
                     /locus_tag="VIN75_06980"
     CDS             complement(1558..1938)
                     /locus_tag="VIN75_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8973943.1"
                     /translation="MLNHARPVCFVATADAGRARAFYEGRLGLALMEDNGYSLVFDAN
                     GIALRVQRVEAVAPHAYTALGWEVDDLVASVHALVAKGVPMLRFPGLAQDADGAWTTP
                     DGARVAWFHDPDGNTLSITQPAAA"
     gene            complement(1984..2550)
                     /locus_tag="VIN75_06985"
     CDS             complement(1984..2550)
                     /locus_tag="VIN75_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973944.1"
                     /translation="MNITSAASLAASVLALSLAGCASGPDTAKGAAKEGVQLQKAVEA
                     PLADLNLVREKIPAVLLTAVKAPYALPSQRDCPALANEVAALDGALGADLDTPPTASN
                     PGLLERGGNMIGDAANDALRGAAESVIPFRGWIRKLDGAERHSKEVAAAIAAGSVRRA
                     FLKGVGQTLGCAAPAAPLQAQAASATQS"
     gene            complement(2547..3014)
                     /locus_tag="VIN75_06990"
     CDS             complement(2547..3014)
                     /locus_tag="VIN75_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEY8973945.1"
                     /translation="MTAATNALHPVDTRRRLAALAAMGITIAPGLASRRWPTALPAVL
                     GKYPGDALWALMVFFGWRALRAGARTRDVALPAMTTCAAVECAKLWQAPWLADFRGTT
                     IGHPLLGHVFSWQNLVAYAVGVLAGAAVDWLLFEPARHERSTGAGRTLIRSRR"
     gene            complement(3011..3811)
                     /locus_tag="VIN75_06995"
     CDS             complement(3011..3811)
                     /locus_tag="VIN75_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8973946.1"
                     /translation="MTARPDETWTGERCREGELGRANRRRIDGRIAWAAMIDKARARH
                     ERLGRAAGALTLGKPGVVSTGSRRKRPAQALCFDTVYARRPVNASGVERRQAGRPSTR
                     AKRPKMRHFAQTVPMHAAIELTSYQPPFRERLVALWRASFAHGVGAPVPHPLEDHLRF
                     LDDHLLMETTVHLALRDGELVGFGAFTPEAVMQLYVAVAHLGQGIGSRLLDRAKADLH
                     GSLWLYTFVTNTNAQRFYERHGFDVVERGFEPVMQLGDLRYEWRRPGR"
     gene            3824..>6912
                     /locus_tag="VIN75_07000"
     CDS             3824..>6912
                     /locus_tag="VIN75_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8973947.1"
                     /translation="MATLPAYAELHCRSNFSFLTGASAPEELVERAAALRYAALAVTD
                     ECSLSGVVHAYEQASERGLPLIIGAEMRLEASVGGVPMRLVLLATTRRGYGNLSQWIT
                     VARRRAEKGRYDARTSDLEGRTPTLPQLAGLPDCLALLLPTPGPVPASAAGTGPTGTD
                     ASGRPGAGPGEVGAPADARVSGMGVIAAAQASAAAALASSTPGAGRPRGREVRESRDG
                     PGGDGPLEPPAPAKLAAAAAPRRPAKAVAPPRPSMALPFESLFAQAQWLKTWFGADRA
                     WLALPLLMQGDDDEVRERVKAVSAATGLRITAVGDVLMATRAAKPLQDMLTATRLQRT
                     VSECGHALSPNAEAHLRSRARLQALYEPEWLAETVAIAGRCKFSLGELKYEYPQEIVP
                     PGKTPTSWLRALTEAGAARRFPAGIPAKVSGLIEHELGLIERLKYEAYFLTVADIVGW
                     ARARGILCQGRGSAANSAVCYCLGVTEVDPHQGEVLFERFISEERGEPPDIDLDFEHQ
                     RREEVIQYLYKKYGRHRAALTGVIIRYRIKSAVRDVGRALGVDLDRIDAVARSLHHVG
                     GSAAPAGAAERLALSLKEHGFDPESRLGLQWISLSLQLRNQPRHLSQHPGGFVIAKDD
                     VALLVPVENAAMDKRTVIQWDKDDLDVLKLLKVDILALGMLSALRRGLLFVDAKLRQL
                     LPGPPPPEWHLEARREEQRENQRRTEVVPVGNAGNSATRITLNARAFSSAGAAAAMGI
                     VADRQGLASDASLADIPDDDKPVYDMLSTGDSVGVFQVESRAQMSMLPRLRPKEFYDL
                     VVEVAIVRPGPIQGGMVHPYLKRREQKEPVTYPSEEAKAALSRTLGVPIFQEQVMQLA
                     ILAADFTPGEADQLRRAMAAWKRKGGLGPFKEKLISRMVARKYTADYAEAIFKQIEGF
                     GEYGFPESHAASFAKLVYASAWIKRHHPDAFLAALLNSLPMGFYAAPQLVRDARAHGV
                     AVRPVDVTISDWESTLEPQAAHEAVPACAERAIPAGQCAVRLGLNRVTGLSEDEGLR"
BASE COUNT          985 a         2438 c         2479 g         1010 t
ORIGIN      
        1 agaatgtcgc tttcatcgct tcttcccgtg cgccaccatg agcatcccca ccgccgccga
       61 actcaaccgc atgggcgccg actgcctgcc cggccacctg ggcgtcgcca tcacgcacgt
      121 ggacgccggc gaactgcgcg ccgaactggc ggtgtcgatg aagctgatgg cgcccaacgg
      181 ctacctgcac gccggcagcg tggtgacgtt ggccgacacc tcctgcggct acgcctgcat
      241 cgccaacctg cccgagggcg cgacgggctt caccacgatc gagcagaaat ccaacttcct
      301 gggcacggcg cgcgagggca cgctcgattg cgtggccacc gcccagcacc gcggccgcac
      361 gacgcaggtc tgggacgcgg tggtgacgca ccgcgagacg ggccggacga tcgcgctgtt
      421 ccgatgcacg cagatgatcc tgtggccgca atcggcctga ctcgcagatc accgtgaccg
      481 atcacctgaa aagggctcac gcatgacaga ttcgtccacg cccgtgcgcc gcctcgtcga
      541 gatccgcgcc tatcgcctca agcccggcac gcgggccgac ttccacgtcg ccgccagcga
      601 gctcgcgctg ccgatggtgc gcgcctatgg catggacgtg gtcgcccacg ggccggtgcc
      661 caatgatccg aacggctatt tcctggtgcg ctcgttcgcc agcctggcgc agttgacggc
      721 gcaggaggac gagttctacg gctcggtccg ctggcgcgag gggccgcgcg aggcgctggt
      781 atcgcgcatc gagacgtacg tcgacacctt gctgtggctg agcccggccg cgatcgacga
      841 cctgcgggcc ggcaacgtgg cgcctgccgt ggtgccctga gcgcgctgga cgccaacagg
      901 ctcacgcgca caggcgcagg agcgcagcgc cgtcgctgat gcggagcgcg gacgtccgcc
      961 caggggccgc gcacgacgcg tcacgcgcgc tcgcgtgcga gccgcgtgcg tctcagtccg
     1021 ccgacttgtc cgcgacggcc gccgccggag cctgcgcttc ggccggcgcc tggtcgtcct
     1081 gggccccggc atgcgatccg gccgccttct tcagcgcctt gtcttccttc ttctgcttct
     1141 tttcgagttc gcgctggcgc ttctcatatg cgtagttcgg tttcgccatg gaatcctttc
     1201 agtgattgca tccaccatac ccgaatgcgc ggcgctgaca agcgtgtcag ggtcaatgcg
     1261 gtcggcgtgg gagcgatttc accccgataa gtccggtgat ggccgcgggc gccggccgtt
     1321 ccgtggtgcc caggcgacgt gtcgcgtcag atcgaatcgc gcggtgcgcc ctggtcgcac
     1381 ttgcggcagg cgagcgtgcg gccgcgcatc gcggcgcgtc cttcgggggt ctgcgtccag
     1441 gggcggttct gccatggcgg gtcgtggcgc acgtgctgga agtggccgca cgcgagctcg
     1501 gccacccagt ggccctcgtc gtcgaggtgg tagccgacga tggcctggtc catgcgctca
     1561 ggcggcggcc ggctgcgtga tcgacagcgt gttgccgtcc ggatcgtgga accaggccac
     1621 gcgcgcgccg tcgggtgtcg tccaggcgcc gtcggcgtcc tgggccaggc ccggaaagcg
     1681 cagcatgggc acgccctttg cgacgagtgc gtggacgctg gccacgaggt cgtccacctc
     1741 ccagccgagc gcggtgtacg catgcggcgc gacggcctcg acgcgctgca cgcgcagcgc
     1801 gatgccgttg gcgtcgaaca ccagcgagta cccgttgtcc tccatcagcg cgagccccag
     1861 ccggccctcg tagaacgcac gggcgcgacc tgcgtcggcc gtggcgacga agcagacggg
     1921 gcgggcatga ttcagcatgg cggactccgc tgggtggggc ggcaagcggc ggtccgccgt
     1981 cgctcagctc tgggtcgccg atgccgcctg cgcctgcagc ggcgcggcgg gcgctgcgca
     2041 gcccagcgtc tggccgacgc ccttgaggaa cgcgcgccgt accgaacccg cggcgatcgc
     2101 ggccgcgacc tccttggagt ggcgctcggc gccgtcgagc ttgcgaatcc agccgcggaa
     2161 cggaatgacg ctctcggcgg cgccgcgcaa cgcatcgttg gcggcgtccc cgatcatgtt
     2221 gccgccgcgt tccagcaggc cggggttgct ggccgtgggc ggggtgtcga ggtccgcgcc
     2281 gagcgcgccg tccagcgcgg ccacctcgtt ggccagcgcg gggcaatcgc gctgcgacgg
     2341 cagggcgtag ggcgccttga cggccgtgag cagcacggcg gggatcttct cgcgcaccag
     2401 gttgagatcg gccagcggcg cctcgacggc cttctgcagc tggacgcctt ccttggccgc
     2461 gcccttggcg gtgtccggcc cggacgcgca gccggcgagc gagagcgcca gcacgctggc
     2521 ggcgagggat gcggcggagg tgatgttcat cgtcgcgagc gtatcagtgt tcggccggct
     2581 cctgtcgagc gttcatggcg cgcgggttcg aacaggagcc agtcgaccgc ggcaccggcc
     2641 agcacgccca cggcataggc cacgaggttc tgccacgaga acacgtggcc cagcagcggg
     2701 tggccgatgg tcgtgccgcg gaagtcggcc agccagggtg cttgccacag cttcgcgcat
     2761 tccaccgccg cgcaggtggt catcgccggc agcgcgacgt cgcgcgtgcg ggcgccggcg
     2821 cgcagcgcgc gccagccgaa gaagaccatg agtgcccaca gcgcatcgcc ggggtacttg
     2881 cccaggacgg cgggcaatgc cgtcggccag cggcgagagg ccaggcccgg cgcgatggtg
     2941 atgcccatcg cggcgagggc ggcgagccgg cggcgcgtgt cgaccgggtg cagggcgttc
     3001 gtggcagcgg tcatcgcccg ggacgcctcc attcgtagcg caggtcgccc agctgcatca
     3061 cgggctcgaa gccgcgctcg acgacgtcga acccgtgacg ttcgtagaag cgctgcgcgt
     3121 tcgtgttggt gacgaaggtg tagagccaga gactgccgtg caaatcggcc ttcgcgcgat
     3181 cgagcaggcg gctgccgatg ccctggccca ggtgcgcgac ggctacgtac aactgcatca
     3241 cggcctcggg cgtgaaggca ccgaagccga ccagttcgcc atcgcgcaat gccaggtgca
     3301 ccgtggtttc catgagcagg tggtcgtcga ggaagcgcag gtgatcctcc agcgggtggg
     3361 gcaccggcgc gccgacgccg tgcgcgaagc tggcgcgcca cagggcgacc aggcgttccc
     3421 ggaacggcgg ttggtaggac gttaactcga tggcggcgtg catggggacg gtctgcgcga
     3481 aatggcgcat tttcgggcgt ttggcgcggg tggacggccg tccagcctgc cggcgctcga
     3541 caccgcttgc attgacgggt ctgcgggcat agactgtatc gaaacacagt gcttgcgcag
     3601 gtcgctttct cctgctgcca gtcgacacca cgcccggctt gccgagcgtc agcgcgcctg
     3661 ccgcgcgacc cagccgctcg tgccgcgcac gagccttgtc gatcatcgcc gcccaggcga
     3721 ttcgtccgtc gatccggcgg cgattcgcgc ggcccagctc tccttcccga cacctttccc
     3781 ccgtccaggt ttcgtccggc cgggccgtca acgtctctcc gccatggcca cgctacccgc
     3841 ttacgccgaa ctgcactgcc gcagcaactt cagcttcctc accggcgcct cggcgcccga
     3901 ggaactggtg gagcgcgccg ccgcgctgcg ctacgctgcg ctggcggtca ccgacgagtg
     3961 ctctctgtcg ggcgtggtgc acgcctacga gcaggccagc gagcggggcc tgccgctgat
     4021 cattggcgcc gagatgcggc tcgaagccag tgtcggcggc gtgccgatgc gcctggtgct
     4081 gctggccacc acgcggcggg gctacggcaa cctgtcgcag tggatcaccg tcgcgcgccg
     4141 gcgtgccgag aagggccgct atgacgcccg caccagcgac ctggagggcc gcacgcccac
     4201 gctaccgcag ctggcgggcc tgcccgattg cctggcgctg ctgctgccca cgccgggtcc
     4261 cgtgccggcc agtgccgcgg ggacgggacc cacgggcacc gacgcgagcg ggcggcctgg
     4321 tgcggggccc ggcgaagtcg gcgcgccggc ggatgcacgc gtgtcgggca tgggcgtcat
     4381 cgccgccgcg caggcgagcg ccgcggccgc gctggcctcc agcacgccgg gagccggccg
     4441 cccgcgcggc cgcgaggtgc gcgagagccg cgacgggccc ggcggcgacg gcccgctcga
     4501 gccgccggcg ccggccaagc tggcggccgc tgcggcgccg cggcggcccg ccaaggcggt
     4561 ggcgccgcca cggccatcga tggccttgcc gttcgagtcg ctgttcgcgc aggcgcaatg
     4621 gctcaagacc tggttcggcg ccgaccgcgc ctggctcgcg ctgcccttgc tgatgcaggg
     4681 cgacgacgac gaggtgcgcg aacgcgtgaa ggcggtgtcg gccgccaccg gcctgcgcat
     4741 caccgcggtg ggcgacgtgc tgatggccac gcgcgcggcc aagccgctgc aggacatgct
     4801 cacggccacg cgcctgcagc gcaccgtgtc cgaatgcggc cacgcgctgt cgcccaacgc
     4861 cgaggcgcac ctgcgatcgc gcgcccggct gcaggcgctg tatgaacccg agtggctggc
     4921 cgagacggtg gccatcgcgg gccgctgcaa gttctcgctc ggcgagctga agtacgagta
     4981 cccgcaggag atcgtgcccc ccggcaagac gcccaccagc tggctgcgcg cgcttaccga
     5041 ggccggcgcc gcgaggcgct ttcccgccgg cattcccgcc aaggtgagcg gcctcatcga
     5101 gcacgagctc gggctgatcg agcggctgaa atacgaagcc tacttcctca cggtggccga
     5161 catcgtcggc tgggcgcgtg cgcgcggcat cctgtgccag ggccgcggca gcgcggccaa
     5221 ttcggcggtc tgctactgcc tgggcgtcac cgaagtcgat ccgcaccagg gcgaggtgct
     5281 gttcgagcgc ttcatcagcg aggagcgcgg cgagccgccc gacatcgacc tcgacttcga
     5341 gcaccagagg cgcgaggagg tgatccagta cctctacaag aaatacggac gccatcgggc
     5401 ggcgctcacc ggcgtcatca tccgctatcg catcaagtcg gcggtgcgcg acgtcgggcg
     5461 cgcgctcggt gtcgacctcg atcgcatcga cgcggtcgcg cgctcgctgc atcatgtggg
     5521 cgggtccgcg gcgccggccg gcgcggccga gcggctcgcg ctgtcgctga aggaacacgg
     5581 cttcgatccc gagtcgcggc tgggcctgca gtggatctcg ctgagcctgc aactgcgcaa
     5641 ccagccgcgc cacctcagcc agcatccggg cggcttcgtc atcgccaagg acgacgtcgc
     5701 gctgcttgtg ccggtggaga acgccgcgat ggacaagcgc accgtgatcc agtgggacaa
     5761 ggacgacctc gacgtcctca agctgctcaa ggtcgacatt ctcgcgctgg gcatgctcag
     5821 cgcgttgcgg cggggcctgt tgttcgtcga tgccaagctg cgccagctgt tgccgggacc
     5881 gccgccgccc gagtggcatc tggaggccag gcgcgaggag caacgcgaga accaacggcg
     5941 caccgaggtc gtccccgtcg gcaatgcggg caacagcgcg acgcgcatca cgttgaacgc
     6001 cagggcgttc tccagcgccg gcgcggcggc cgcgatgggc atcgtcgcgg accgccaggg
     6061 cctggccagc gacgccagcc tggccgacat ccctgacgac gacaaaccgg tctacgacat
     6121 gctgtccacc ggcgattccg tcggtgtgtt ccaggtcgag agccgcgcgc agatgagcat
     6181 gctgccgcgg ctgcggccca aggagttcta cgacctggtg gtggaggtgg ccatcgtgcg
     6241 gcccgggccg atccagggcg gcatggtgca tccctacctg aagcggcgcg agcagaagga
     6301 gcccgtcacc tatcccagcg aggaggcgaa ggcggcgttg tcgcgcacgc tgggcgtacc
     6361 catcttccag gagcaggtga tgcagctggc catcctcgcc gccgacttca cacccggcga
     6421 ggccgaccag ttgcggcgtg cgatggcagc ctggaagcgc aagggcggcc tcgggccgtt
     6481 caaggagaag ctgatcagcc gcatggtggc gcgcaagtac acggccgact acgccgaggc
     6541 catcttcaag cagatcgagg gctttggcga gtacggcttc cccgagagcc acgccgccag
     6601 cttcgcgaag ctggtctacg ccagcgcctg gatcaagcgc caccatcccg acgccttcct
     6661 ggccgcgctc ctcaacagcc tgccgatggg cttctacgcc gcgccccagc tcgtgcggga
     6721 cgcgcgcgcg catggcgtcg cggtgcgtcc cgtcgacgtc accatcagcg actgggagtc
     6781 cacgctggag ccgcaagccg cccacgaagc ggttccggcc tgtgccgaac gtgcgattcc
     6841 cgcgggccag tgcgcggtgc gcctggggtt gaaccgggtc accgggctgt ccgaggacga
     6901 agggctgcgc at
//
LOCUS       DATCRO010000257         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_47883, whole genome shotgun sequence.
ACCESSION   DATCRO010000257 DATCRO010000000
VERSION     DATCRO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47883"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3741)
                     /locus_tag="VIN75_07005"
     CDS             complement(<1..>3741)
                     /locus_tag="VIN75_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEY8973948.1"
                     /translation="SFSDREIVLRLLGSRAWEILNRLREERRTGRSARMLYEVLGDIW
                     VVQRNPYLEDDLLDNPKRRALLIDALNHRLAEVEKRRTPDADAARDAFVAELLDGARR
                     CVADFARRFSDVWDLRQRTRKVLGKVTAKDNIKFDGLSRVSHVTDATDWRVEYPFVVL
                     TPDTEAEMAALVAGCVELGLTIVPRGGGTGYTGGAVPLTWMSAVINTEKLEAMTEVEM
                     VAIPGVDHPVGTVWTEAGVVTQRVSDAADRAGFVFAVDPTSAEASCIGGNIAMNAGGK
                     KAVLWGTALDNLVSWRMVTPDAKWLEVTRLDHNLGKIHDAPVARFELKYFDASGKKLE
                     RTETLEIPGRQFRKEGLGKDVTDKFLAGLPGIQKEGTDGLITSARWVVHRMPAHVRTV
                     CLEFFGNPKEAVPSIVEIKDFMFAEARKPGGAILAGLEHLDDRYLRAVGYATKSKRGG
                     LPKMALFGDIVGDDEDAVARATSEVVRLANARSGEGFVAVSADARKKFWLDRKRTAAI
                     ARHTNAFKVNEDVVIPLERMGEYTLGIERINIELSLRNKLQLLDRLEDFLSASPLPLG
                     KSEDTQAIPSAEILGDRAARALVLLAGVRALWRGWMTGLDVVQPAVGDEPARTFFERI
                     QDVTLRASWKTQVLAPLQQIFAGAEFMPIIEECRRIHKDVLRGRTWVALHMHAGDGNV
                     HTNIPVNSDNYEMLQTAHEAVARIMVLARSLNGVISGEHGIGITKLEFLSDDEIADFT
                     AYKQRVDPQGRFNKGKLLRGAPLAALGGDVRHADLSHAYTPSFGLMGHESLIMQQSDI
                     GAISASIKDCLRCGKCKPVCATHVPRANLLYSPRNKILATSLLIEAFLYEEQTRRGVS
                     LRHWEEFEDVGDHCTVCHKCVTPCPVDIDFGDVSMNMRNLLRKMGQKTFRPGNAAAMF
                     MLNATNPQTIKIARTAMVQVGMKAQRAANNVFKIAARHQTAAPPATVGAATVKEQVIH
                     FVNKKMPGGLPKKTARALLDIEDKNYVPIIRDPKKTTAETEAVFYFPGCGSERLFSQV
                     GLATQAMLWHAGVQTVLPPGYLCCGYPQRGSGQFDRADEIITDNRVLFHRVANTLNYL
                     DIKTVVVSCGTCYDQLQGYKFEDIFPGCRIVDIHEYLLEKGITLDAGPGYLYHDPCHT
                     PMKLQDPMKTVKALVGDAVVKSARCCGESGTLGVSRPDISTQVRFRKEEELRKDETAL
                     RAIQAAQGLAKPDADIKILTSCPSCLQGLSRYGEDLKTGLLEA"
BASE COUNT          506 a         1293 c         1300 g          642 t
ORIGIN      
        1 ggcctccagc aggcccgtct tcaggtcctc gccgtagcgc gacaggccct gcaggcaact
       61 ggggcacgag gtcaggatct tgatgtcggc gtccggcttg gccaggccct gcgcggcctg
      121 gatcgcgcgc agcgccgtct cgtccttgcg cagctcctct tccttgcgga atcgcacctg
      181 cgtggagatg tcgggccgcg agacgcccag cgtgcccgac tcgccgcagc accgcgcgct
      241 cttgacgacg gcgtcgccca ccagcgcctt gaccgtcttc atcggatcct gcagcttcat
      301 cggcgtgtgg cagggatcgt ggtacaggta gcccggcccc gcgtccagcg tgatgccctt
      361 ctcgagcagg tactcgtgga tgtcgacgat gcggcagccc gggaagatgt cctcgaactt
      421 gtagccctgc aactggtcgt agcacgtgcc gcagctgacc accaccgtct tgatgtccag
      481 gtagttgagc gtgttggcca cgcggtggaa cagcacgcgg ttgtcggtga tgatctcgtc
      541 ggcgcggtcg aactgccccg agccgcgctg cggatagccg cagcacaggt agcccggggg
      601 cagcacggtc tgcacgccgg cgtgccacag catcgcctgc gtggccaggc ccacctgcga
      661 gaacaggcgc tcggagccgc agcccgggaa gtagaagacg gcctcggtct cggccgtcgt
      721 cttcttcggg tcgcggatga tcgggacgta gttcttgtcc tcgatgtcca gcagcgcgcg
      781 cgccgtcttc ttgggcaggc cgcccggcat cttcttgttg acgaagtgga tgacctgttc
      841 cttgaccgtg gccgcgccga ccgtggccgg cggcgccgcg gtctggtggc gcgccgcgat
      901 cttgaacacg ttgttggccg cgcgctgcgc cttcatgccc acctggacca tcgcggtgcg
      961 cgcgatcttg atcgtctgcg gattggtggc gttgagcatg aacatcgccg cggcgttgcc
     1021 ggggcggaag gtcttctggc ccatcttgcg cagcaggttg cgcatgttca tcgagacgtc
     1081 gccgaagtcg atgtcgaccg ggcacggcgt gacgcacttg tggcacacgg tgcagtggtc
     1141 gccgacgtcc tcgaactcct cccagtggcg cagcgacacg ccgcggcgcg tctgctcctc
     1201 gtacaggaag gcctcgatca gcagcgaggt cgccaggatc ttgttgcgcg ggctgtacag
     1261 caggttggcg cgcggcacgt gcgtggcgca caccggcttg cacttgccgc agcgcaggca
     1321 gtccttgatc gacgcgctga tggcgccgat gtccgactgc tgcatgatca gcgactcgtg
     1381 ccccatcagg ccgaacgacg gcgtgtacgc gtgcgacagg tcggcgtggc gcacgtcgcc
     1441 gccgagcgcg gccagcggcg cgccgcgcag cagcttgccc ttgttgaagc ggccctgcgg
     1501 atcgacgcgc tgcttgtagg ccgtgaagtc ggcgatctcg tcgtcggaca ggaactcgag
     1561 cttggtgatg ccgatgccgt gctcgcccga gatgacgccg ttcaggctgc gcgccagcac
     1621 catgatgcgc gcgaccgcct cgtgcgcggt ctgcagcatc tcgtagttgt cggagttgac
     1681 cgggatgttg gtgtggacgt tgccgtcgcc ggcgtgcatg tgcagcgcca cccaggtgcg
     1741 cccgcgcagc acgtccttgt ggatgcggcg gcactcctcg atgatgggca tgaactcggc
     1801 gcccgcgaag atctgctgca gcggcgcgag cacctgcgtc ttccacgagg cgcgcagcgt
     1861 cacgtcctgg atgcgctcga agaacgtgcg cgcgggttcg tcgccgaccg ccggctgcac
     1921 gacgtccagg ccggtcatcc agccgcgcca cagcgcgcgc acgccggcca gcagcaccag
     1981 cgcgcgggcc gcgcggtcgc ccaggatctc ggcgctcggg atggcctgcg tgtcctcgct
     2041 cttgcccagc ggcagcgggc tggccgacag gaagtcctcg aggcggtcca gcagctgcag
     2101 cttgttgcgc aggctcagct cgatgttgat gcgctcgatg cccagcgtgt actcgcccat
     2161 gcgctccagc gggatcacca cgtcctcgtt caccttgaac gcgttggtgt gcctggcgat
     2221 ggcggccgtg cgcttgcggt cgagccagaa cttcttgcgc gcgtccgcgc tgacggccac
     2281 gaagccctcg cccgagcgcg cgttggcaag gcgcacgacc tcgctggtgg cgcgcgcgac
     2341 ggcgtcctcg tcgtcgccga cgatgtcgcc gaacagcgcc atcttgggca gcccgccgcg
     2401 cttgctcttg gtggcgtagc ccaccgcgcg caggtagcgg tcgtcgaggt gctcgaggcc
     2461 ggccaggatc gcgccgccgg gcttcctggc ctcggcgaac atgaagtcct tgatctcgac
     2521 gatggagggc accgcttcct tcgggttgcc gaagaactcc aggcacaccg tgcgcacgtg
     2581 cgccggcatg cggtgcacga cccagcgcgc gctggtgatc agcccgtcgg tgccttcctt
     2641 ctggatgccg ggcaggccgg cgaggaactt gtcggtgacg tccttgccca ggccttcctt
     2701 gcggaactgg cgccccggga tctccagcgt ctccgtgcgc tcgagcttct tgcccgacgc
     2761 gtcgaagtac ttgagctcga agcgcgcgac gggcgcgtcg tggatcttcc cgaggttgtg
     2821 gtccaggcgg gtgacctcca gccacttggc gtccggcgtg accatgcgcc agctcacgag
     2881 gttgtccagc gcggtgcccc acagcacggc cttcttgccg cccgcgttca tcgcgatgtt
     2941 gccgccgatg cagctcgcct cggcggacgt cgggtcgacc gcgaacacga agcccgcgcg
     3001 gtcggccgcg tcggacacgc gctgcgtcac cacgcccgct tccgtccaca ccgtgcccac
     3061 cggatggtcg acgcccggga tcgcgaccat ctcgacctcg gtcatcgcct cgagcttctc
     3121 cgtgttgatc accgcgctca tccaggtgag cggcacggcg ccgccggtgt agcccgtgcc
     3181 gccgccgcgc gggacgatcg tcagccccag ctcgacgcag ccggccacca gcgcggccat
     3241 ctcggcctcg gtgtcgggcg tgagcacgac gaacgggtac tcgacgcgcc agtcggtggc
     3301 gtcggtgacg tgcgagacgc gcgacagccc gtcgaacttg atgttgtcct tggcggtgac
     3361 cttgcccagc accttgcgcg tgcgctggcg caggtcccac acgtcgctga agcgcctggc
     3421 gaagtcggcg acgcagcggc gcgcgccatc gagcagctcg gcgacgaagg cgtcgcgcgc
     3481 ggcgtcggcg tcgggcgtgc ggcgcttctc gacctcggcc aggcggtggt tcagcgcgtc
     3541 gatcagcagc gcgcggcgct tcgggttgtc gaggaggtcg tcctcgaggt acgggttgcg
     3601 ctgcaccacc cagatgtcgc ccagcacctc gtagagcatg cgcgccgagc ggccggtgcg
     3661 gcgctcctcg cgcaggcggt tcaggatctc ccaggcccgg gagccgagca ggcgcagcac
     3721 gatctcgcga tcggagaacg a
//
LOCUS       DATCRO010000258        10673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1015187, whole genome shotgun sequence.
ACCESSION   DATCRO010000258 DATCRO010000000
VERSION     DATCRO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015187"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VIN75_07010"
     CDS             complement(<1..640)
                     /locus_tag="VIN75_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8973949.1"
                     /translation="MRDLTQGSIRGHLIAMAIPITVSMLVQTLYLMVDLYFVSGTGKE
                     AVAGVAAAGNITLLVMALTQMLGVGTVSLVAQALGAKDRTRASNVFRQSMLLGAACVV
                     ATIALGVVFAPAYMRTVAADAAITQAGVTFILCSLPGLALQFVLAVAGSVLRGAGVTR
                     PGMVVQLATVGLNVVLAPVLIAGWGTGHPLGVAGAGLATSVSVAVGVLMLTRY"
     gene            complement(733..1404)
                     /locus_tag="VIN75_07015"
     CDS             complement(733..1404)
                     /locus_tag="VIN75_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY8973950.1"
                     /translation="MTRTALPYSAPDLSALARLLERALLDHQVTHGRLPGHVEMMNLL
                     ARGAGHRNLQALQAAVAPAPAAAAPVTADAWFDAPAIDEPATPTAPAAPVLSAHARKA
                     LEQFDAAGRLEHWPPKFSVQRLVVWVLWTRFDARRVYTEAEVNKILKGWHTYGDHVTL
                     RRELINHRLMTRKSDCSEYRKLALEPDDEVRSLLQALRSAARTRRRGRGPRPPRELPR
                     TAQAA"
     gene            1891..3219
                     /locus_tag="VIN75_07020"
     CDS             1891..3219
                     /locus_tag="VIN75_07020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8973951.1"
                     /translation="MKRISWFLVLLVLTAAALLWNEYGMERHLSLDGRWRSLQSVDDS
                     SSGGKSSSSVRRDGNDLVMDCDIRAGYAWPYCTLVFELARPPKGVDLSAYGSMTVEAS
                     YDGPEKMKQLRLFLLNFNPLYSRVGQDGSDKVQEFVYDSQARPVLNARLAQFAVSSWW
                     ANEHPVDAEHSGPEMDNIVAVQVSTGGNVQPGHHELRLHRIDFVGKVIPPATFRLGVI
                     AFWGVLALAWSLLHALRLRRDLDLTRHGKAALESLNKQLREDARHLTNVAKRDALTGV
                     LNRHGLRDELARAAERGDERFFPVAIVFVDIDHFKRINDTHGHAVGDQVITQIADVIC
                     SAVQRDDLCARWGGEEFLLIFPGTKVSDARAIAERLRRALHQREWPMGMQVTGSFGVA
                     QASAGEDLVEGIRRADEAMYAAKSQGRDRVEMATGSGVPSLPPPAVPESV"
     gene            complement(3589..4011)
                     /locus_tag="VIN75_07025"
     CDS             complement(3589..4011)
                     /locus_tag="VIN75_07025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8973952.1"
                     /translation="MIAASTAPAASSDHLNQSEAHMTTTPSGLQYEDTKVGDGPVAKA
                     GQSVTVHYTGWLSDPSAPNGRGSKFDSSKDRGQPFVFGLGQGMVIKGWDEGVAGMAAG
                     GTRVLTIPPELGYGARGAGGVIPPNATLVFEVDLISSK"
     gene            complement(4036..4962)
                     /gene="gluQRS"
                     /locus_tag="VIN75_07030"
                     /pseudo
     CDS             complement(4036..4962)
                     /gene="gluQRS"
                     /locus_tag="VIN75_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
     gene            complement(5126..6127)
                     /locus_tag="VIN75_07035"
     CDS             complement(5126..6127)
                     /locus_tag="VIN75_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8973953.1"
                     /translation="MSTDRRFALTPEALTMMDTIARTGSFAAAARELGKVPSALTYSV
                     RQLEDALDVLLFDRSSRQARLTAAGTELLNEGRRLLAEMDAVANRVHRVSTGWETQLT
                     IAADAVVSRQTLFELCEAFYALRPPELHDGAGPGTRLRLRTEVLAGTTEALLLGSADL
                     AIGLPADTLAPAGIEIRPLGDAPFVYVVAPHHPLAKAAEPISDAVLRQHRGVAVADSA
                     QRMSPITVNIQPGQDVLTVSSMQAKLEAQMMGLGAGFLPEVLVREHVAAGRLVVKEVA
                     RQRMTASLSYGWRSAAAPQPQKAPQGLALQWWLQQLESPTTRSALLLRGGPCPPAES"
     gene            complement(6124..7119)
                     /locus_tag="VIN75_07040"
     CDS             complement(6124..7119)
                     /locus_tag="VIN75_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8973954.1"
                     /translation="MQALVRAFGVLLMLTALAVTLAHAPDRSVDSLVTRWAPPPSDFM
                     YVKGQFVHFRDEGPKNDPLPLVLIHGTASSLHTWQGWAGDLRARKRVITFDLPGFGLT
                     GPFTGQYPADDYHADNLASFTLDFLDALHVQRVAIGGNSLGGEVAWRVAAMAPARVDR
                     LILVDATGYAFVPEHIPVGFQLARVKGLNRLGEFLTPRSVVEDSVRDVYGDPSRVTGA
                     LVDRYFEMMMREGNRHALNLRMEVIASDLAPERIKTLKLPTLILWGGKDRLVPPVNAQ
                     RFQGDIAGSRLVVFPALGHVPQEEDAPQSVAPVRAFLGLDKPADDAPAAPTKATP"
     gene            complement(7121..7903)
                     /locus_tag="VIN75_07045"
     CDS             complement(7121..7903)
                     /locus_tag="VIN75_07045"
                     /inference="COORDINATES: protein motif:HMM:NF003703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8973955.1"
                     /translation="MQFLNISTYRFVALDDLPALRDRLEASAAAAGLKGTVLLAHEGI
                     NLFMAGDEDALRGWVAGALHAEPPFAGLPTKDSFSVAMPFKRLKVKVKNELIRMNHPT
                     IKPQDGRAASVTPATLARWLAQGHDDDGRAVAMLDTRNAFEVDAGTFEGTHDWRIAKF
                     SDFPAAFDAHRAELAGKTVVSFCTGGIRCEKAALYMRERGLPDVLQLEGGILGYFEAT
                     AGAAPGWRGECFVFDERRTVDPELAPGDPPPCERSGAVAEPR"
     gene            8057..8482
                     /locus_tag="VIN75_07050"
     CDS             8057..8482
                     /locus_tag="VIN75_07050"
                     /inference="COORDINATES: protein motif:HMM:NF040519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sbal_3080 family lipoprotein"
                     /protein_id="HEY8973956.1"
                     /translation="MRIVRALACLAATAGCTSIQVKPLDASSPLKQVCIVQNPRVEVS
                     DFVDVLRDGFDRHGIASSVVASEDAKPCDATLTYTALRTWDLAPYLSHAELRLWRDGR
                     QIGSATYHLNGGGGLSLMKWQGTKTKMDPVIDQLLAAKP"
     gene            8585..>10673
                     /gene="dnaE"
                     /locus_tag="VIN75_07055"
     CDS             8585..>10673
                     /gene="dnaE"
                     /locus_tag="VIN75_07055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8973957.1"
                     /translation="MAFVHLRTHTEFSVVDGTLRIDDVAAAAKGDGQVALGITDLSNL
                     FGAVKFYKACRGKGVKPIVGVDVWMDPRPDAADRVPTRLVLLVQSRQGYLNLCELLGR
                     SWTKNVQRAQAWVRWDWLEELGDGMIALSGADHGAVGQALLAGDVPRAEAAALELARI
                     FPGRFYLELQRAGLPNHEPHVRATVPLAARLQLPVVATHPVQFLDPDDFDAHEARVCI
                     AEGETLANPRRVKRFTRDQYFKGQAEMEALFADIPSAIANTVEIARRCNLTLAMGKNY
                     LPDFPTPILADGSAMPMDDYFRQLSFEGLEDRLALLYPDAAVRDAKRPEYIERLEFEI
                     KTILNMGFPGYFLIVSDFIKWAKANGCPVGPGRGSGAGSLVAYVLKITDLDPLQYKLL
                     FERFLNPERVSMPDFDVDFCQGNRDRVIEYVKDKYGRAAVSQIATFGTMAAKAALRDI
                     GRVLGMGYGHVDSIAKLIPAPPGKTVTLAKVPEKPDPGLIYARLEAPELDQREKAEEE
                     VAELLALAARVEGIVRNIGMHAGGVLIAPGKITDFCPLYQQPGSESAVSQFDKDDVEA
                     IGLVKFDFLGLATLTILELAKDFIVARRPGRENFSYETLPLDDPKVYRLFSDGLTEAV
                     FQFESRGMQGMLRDAKPSRLEDLIALNAMFRPGPMDNIPSFCARKHGREEVTYPHPLL
                     EKVLGETYGIMVYQ"
BASE COUNT         1527 a         3681 c         3920 g         1545 t
ORIGIN      
        1 ggtagcgggt gagcatcagc acgcccaccg ccacggacac gctggtggcc aggccggcgc
       61 cggccacgcc cagcgggtgc ccggtgcccc agccggcgat caagaccggc gctagcacga
      121 cgttcaggcc gacggtggcc agctgcacca ccatgcccgg cctggtgacg ccggcgccgc
      181 gcagcacgga gcccgcgacc gccagcacga actgcagcgc caggcccggc agcgagcaca
      241 ggatgaacgt gacgccggcc tgcgtgatcg ccgcgtcggc ggcgacggtg cgcatgtacg
      301 cgggcgcgaa cacgacgccc agcgcgatcg tcgcgacgac gcaggccgcg cccagcagca
      361 tcgattgccg gaagacgttg ctggcgcgcg tgcggtcctt cgcgccgagc gcctgggcca
      421 cgagcgagac ggtgccgacg cccagcatct gcgtcagcgc catcacgagc agggtgatgt
      481 tgcccgccgc ggcgacgccg gcgacggcct ccttgccggt gcccgagacg aagtagaggt
      541 cgaccatgag gtacagcgtc tgcaccagca tgctcacggt gatgggaatg gccatcgcga
      601 tcaggtggcc ccggatggag ccttgggtca ggtcacgcat ttggatgctt tttctcgttg
      661 gattgaaagg gcgtggcggg ccgggtgggc cgccacgcca cgtcgaagcc gtccgacgga
      721 cggcctctct cctcaggccg cctgcgcggt gcgcggcagt tcgcgcggcg gacgcggccc
      781 gcgcccgcgc ctgcgcgtgc gcgcagccga gcgcagcgcc tgcagcagcg agcgcacctc
      841 gtcgtcgggt tccagcgcga gcttgcggta ctcgctgcag tcgctcttgc gggtcatcag
      901 ccgatggttg atgagctcgc ggcgcagcgt cacgtggtcg ccgtaggtgt gccagccctt
      961 gaggatcttg ttgacctcgg cctcggtgta gacgcggcgc gcgtcgaagc gcgtccacag
     1021 cacccacacc accaggcgct gcaccgagaa cttgggcggc cagtgctcga ggcggccggc
     1081 agcatcgaac tgctcgagcg cctttctcgc atgcgcgctc agcacgggcg cggcgggcgc
     1141 cgtcggcgtt gccggctcgt cgatggcggg cgcgtcgaac caggcgtcgg ccgtcaccgg
     1201 cgcggcggcc gcgggcgccg gcgcgaccgc ggcctgcagc gcctgcaggt tgcgatggcc
     1261 tgcgccgcgg gcgagcaggt tcatcatctc cacatggccg ggcagccggc cgtgggtgac
     1321 ctggtggtcg agaagggcgc gctcgagcag gcgggcgagc gcggacaggt cgggggccga
     1381 gtacggcagg gcggttcgag tcatggcaaa gcctcacgtc gtgcgtgcgc tcgatgaagc
     1441 cgcaggggaa tgcggcgccg ggggttgccc ttgccggaat cgagctcacg cgcggtgagt
     1501 caggcccgaa cttggaggcg cggcgaggaa tccggaggaa acgccgtgcg gcaggtttag
     1561 cgacgctgga tgccagcgcg gcaacgcctg ggtgaactgc cgaccgggcg ccagtgtagc
     1621 gcgagccggc acgcgaacgc cagcccgatg cgccgcgggc ggcgaaagcc cgttgcggcg
     1681 cggggccgcg tcgtgcatcg acatgtcgcc atcgaccgga cgaacgtccc gggatcgtcg
     1741 acgggaaatt cccgtgcgga atggccgcac gagggagctg tccgcgtgcc ggatttcatg
     1801 ctggcaatgc atggctcggc cgcaagtctt cagtcggcga cgggttggcc gaagacagag
     1861 gcgtcgtttc ccgcgaccgg cctctttcga atgaagcgca tctcctggtt cctcgtcctg
     1921 ctcgtcctca ccgccgcagc cctgctctgg aacgagtacg gcatggagcg tcacctgagc
     1981 ctggacggcc gttggcgcag cctgcagtcc gtcgacgaca gttcgtccgg cggcaagtcc
     2041 agctcgtcgg tgcgccgcga cgggaacgac ctcgtgatgg actgcgacat ccgcgccggc
     2101 tatgcgtggc cgtactgcac cctggtcttc gagctggccc ggccgcccaa gggcgtcgac
     2161 ctgtcggcct acggttcgat gaccgtcgag gcgtcctacg acggccccga gaagatgaag
     2221 cagctgcggc tgttcctgct gaacttcaac ccgctgtatt ccagggtggg acaggacgga
     2281 tcggacaagg tgcaggagtt cgtctacgac tcgcaggcgc ggccggtgct gaacgcgcgc
     2341 ctcgcgcagt tcgcggtgtc gtcgtggtgg gccaacgagc atccggtgga cgccgagcac
     2401 tccggccccg agatggacaa catcgtcgcc gtgcaggtgt ccaccggcgg caacgtccag
     2461 ccgggacacc atgagctgcg cctgcaccgc atcgacttcg tcggcaaggt gatcccgccc
     2521 gccacgttcc gcctcggcgt gatcgcgttc tggggcgtgc tggcgctggc ctggtcgctg
     2581 ctgcacgcgc tgcggctgcg ccgcgacctg gacctgacgc gccacggcaa ggccgcgctc
     2641 gagagcctga acaagcagct gcgcgaggat gcgcggcacc tgacgaacgt ggccaagcgc
     2701 gacgcgctga ccggcgtgct caaccgccac ggcctgcgcg acgagctggc gcgcgccgcc
     2761 gagcgcggcg acgagcgctt cttcccggtg gctatcgtct tcgtcgacat cgaccacttc
     2821 aagcgtatca acgacacgca cggccacgcg gtgggcgacc aggtgatcac gcagatcgcc
     2881 gacgtgatct gcagcgccgt gcagcgcgac gacctgtgcg cgcgctgggg cggcgaggaa
     2941 ttcctgctga tcttccccgg cacgaaggtc agcgacgcgc gcgccatcgc ggagcgcctg
     3001 cgccgcgcgc tgcaccagcg cgaatggccg atgggcatgc aggtgacggg cagcttcggc
     3061 gtggcccagg ccagcgccgg cgaagacctc gtcgagggca tccgccgcgc cgacgaggcg
     3121 atgtacgcgg ccaagagcca ggggcgcgac cgggtcgaga tggccacggg ctccggcgtg
     3181 ccgtcgctgc ccccgccggc ggtgcccgag tcggtctgag cgaagcggcg ccgttcgacg
     3241 cgcgcgccgg ccatgccgct gctgcctcgc cggccgccat cctgcgcgca gcggcgggat
     3301 ccacagtggc catgagactg gcttgccggg gtggatcctg cgaccccgct tcgcttcgcg
     3361 caggatgacg gcttggcgct cggcgtgacg cgctgctggc gctcggcgtg ccgtgttgtt
     3421 ggcgctcggc gggatgagtt gttgcggccg cgcttgacgc gcctgtcgca tcgcgcccac
     3481 cccgtgtcat cctgcgctca gtcgcaggat ccaccccggc gatgccgcgc cattgcagga
     3541 agggcgtgcc cgacgccggc tgaggccggg cgtcgcgagc tggaagcctt acttcgacga
     3601 gatcagatcg acctcgaaca cgagcgtggc gttgggcgga atgacgccgc cggcgccgcg
     3661 cgcgccgtag cccagttccg gcgggatcgt cagcacgcgg gtgccgccgg cggccatgcc
     3721 ggccacgcct tcgtcccagc ccttgatgac catgccctgg cccaggccga acacgaaggg
     3781 ctggccgcga tccttgctgg agtcgaactt gctgccgcgg ccgttgggcg cggacgggtc
     3841 ggacagccag ccggtgtagt gcacggtgac ggactggccg gccttggcga cggggccgtc
     3901 gccgaccttg gtgtcttcgt attgcaggcc ggagggggtg gtggtcatgt gggcttcgct
     3961 ctggttgagg tgatcggacg acgccgcggg cgccgtggag gcagcgatca tgccaccgac
     4021 cgcgcgcaac ctgccggtcc aggccggcac ggccttggcg agccagcccg acggcgtctc
     4081 gctgtggtcg ctgccgtcgt gcagcgcggc gggcaacccg agcacgcggg cggcgcggcg
     4141 cagcgcgtgc acggcgtcga cgtcgctgcg cagcgcgagc ggccgcgcgc cggtctgctt
     4201 ggacagcttc tggccgtcgt cggcaaacac gagcggcgtg tgcaggtagc gcggcgtcgg
     4261 caggcccagc acgcgttgca ggtggatctg gcgcgccgtg ttgtcggcca ggtcctcgcc
     4321 gcgcacgacg tcggtgacgc cttgcgcggc gtcgtcgacc accaccgcca gctggtaggc
     4381 ccacaggccg tcggcgcgct tgagcacgaa gtcgccgacg gcctgcgtca cgtcctggcg
     4441 ctggtcgccg agcaggcggt cgtgccagtc gacgacgacg tcggcgccgc cgggcgtgcc
     4501 cgtgcgcagg cgccaggcgg gcgtgcgccg gaacgggacc tcgggctcga cgtcggagga
     4561 cgccatcgtg ggcgggcggc acgtgcccgg gtacacgcgc tcgacgtgcg cgacgtgcgg
     4621 gacgcccgcg cgcgcgagcg cggcggcgat gtccgagcgc gagcagacgc acggataggc
     4681 ccagcccgcg cgcttgagct gcgtgagcgc ggcctcgtag cgctcgccgc ggcgcgactg
     4741 ccacaccggc actgcgtcgg gcgccatgcc cagcgcggcc agctgcgcga ggatgccggt
     4801 gtcggcgtcg cgcacgcagc gcggcgtgtc gacgttctcg atgcgtacca gccacgtgcc
     4861 gccgtgggcg cgcgcgtcca gccagctggc gagcgcggcc accagcgagc cggcgtgcag
     4921 caggccggtg ggcgagggcg cgaagcggcc gacgtaggtc atcgggcgat gggcggtggc
     4981 gccgaggatg cgtgaacggg cccatcgcct gcgcgggcgt gcatcggcga gccgtgctgc
     5041 agcgtgccgg cgcgcgaggc gccgcggcga tggacggcgt tcatcgtggc aaatccgggt
     5101 gcgaggaatg cggcaggcgc cgggctcacg attccgccgg cgggcacggg ccgccgcgaa
     5161 gcagcagcgc cgagcgcgtc gtcgggctct cgagctgctg cagccaccat tgcagcgcca
     5221 gcccttgcgg cgccttctgc ggctgcggcg ccgcggcgct gcgccagccg tacgacaggc
     5281 tcgccgtcat gcgctgccgc gccacctcct tgacgaccag ccgcccggcg gccacgtgct
     5341 cgcgcaccag cacctcgggc aggaaaccgg cgcccaggcc catcatctgc gcctcgagct
     5401 tggcctgcat gctgctgacc gtgagcacgt cctgccccgg ctggatgttg acggtgatcg
     5461 gcgacatgcg ctgggccgag tcggccaccg cgacgccgcg gtgctgccgc agcaccgcgt
     5521 cgctgatggg ctctgcggcc ttggcgagcg gatggtgcgg tgcgacgacg tagacgaagg
     5581 gcgcgtcgcc gagcggccgg atctcgatgc ccgcgggcgc gagcgtgtcc gccggcaggc
     5641 cgatggcgag atcggccgat ccgagcagca gcgcctcggt cgtgccggcc agcacctcgg
     5701 tgcgaaggcg cagccgtgtg ccgggccccg cgccgtcgtg cagctcgggt gggcgcagcg
     5761 cgtagaacgc ctcgcacagc tcgaacagcg tctggcgcga caccacggcg tcggccgcga
     5821 tggtcagctg cgtctcccag ccggtggaca cgcggtgcac gcgattggcg acggcgtcca
     5881 tctcggccag caggcgccgg ccctcgttga gcagctccgt gcccgcggcc gtgagccgcg
     5941 cctggcgcga gctgcggtcg aacagcagca cgtcgagcgc gtcctccagc tgccgcacgc
     6001 tgtaggtgag ggcggagggc accttgccca gctcgcgcgc ggcggcggcg aagctgccgg
     6061 tgcgggcgat ggtgtccatc atcgtgagcg cctcgggcgt gagggcgaaa cgacggtcgg
     6121 tgctcatggg gtggccttgg tcggtgctgc gggcgcgtcg tcggccggct tgtccaggcc
     6181 caggaaggcg cgcaccgggg cgacgctctg cggcgcgtcc tcctcctgcg gcacgtggcc
     6241 cagcgcgggg aagacgacca gccggctgcc ggcgatgtcg ccctggaagc gttgggcgtt
     6301 gaccggcggc acgaggcgat ccttgccgcc ccacaggatc agcgtgggca gcttcagcgt
     6361 cttgatccgc tcgggcgcga ggtcgctcgc gatcacctcc atgcgcaggt tcagtgcgtg
     6421 gcggttgccc tcgcgcatca tcatctcgaa gtagcggtcg accagggcgc ccgtgacgcg
     6481 cgacgggtca ccgtagacgt cgcgcacgct gtcttcgacg acggagcgcg gcgtcaggaa
     6541 ctcgccgagc cggttcaggc ccttgacacg cgccagctgg aagccgacgg ggatgtgctc
     6601 gggcacgaag gcgtagccgg tggcatccac gaggatcagc cggtcgacgc gcgccggcgc
     6661 catggccgcg acgcgccacg ccacctcgcc gccgagcgag ttgccgccga tcgccacgcg
     6721 ctgcacgtgc agggcgtcca ggaagtcgag cgtgaagctc gccaggttgt cggcgtggta
     6781 gtcgtcggcc gggtactgcc ccgtgaacgg gccggtgagg ccgaagccgg gcaggtcgaa
     6841 cgtgatgacc cgcttgcggg cgcgcaggtc gcccgcccag ccctgccatg tgtgcaggct
     6901 ggacgcggtg ccgtggatca gcaccagcgg cagcgggtcg ttcttcgggc cctcgtcgcg
     6961 gaagtgcacg aactggccct tgacgtacat gaagtccgac ggcggcggtg cccagcgcgt
     7021 gaccaggctg tcgacgctgc ggtcgggcgc gtgcgcgagc gtgacggcca gcgccgtcag
     7081 catcagcagc acgccgaagg cgcggacgag ggcttgcatg tcagcggggc tccgcgacgg
     7141 cgccggaccg ctcgcacggc ggtgggtcgc cgggggcgag ctcggggtcg acggtgcggc
     7201 gctcgtcgaa cacgaagcac tcgccgcgcc agccgggcgc ggcgccggcg gtggcctcga
     7261 agtagccgag gatgccgccc tcgagctgca ggacgtcggg caggccgcgt tcgcgcatgt
     7321 acagcgccgc cttctcgcag cggatgccgc cggtgcagaa gctcaccacc gtcttgcccg
     7381 cgagctcggc gcggtgcgcg tcgaaggcgg cggggaagtc gctgaacttg gcgatgcgcc
     7441 agtcgtgcgt gccctcgaag gtgccggcgt cgacctcgaa cgcgttgcgg gtgtcgagca
     7501 tcgccaccgc gcggccgtcg tcgtcatggc cttgcgcgag ccagcgcgcg agcgtggccg
     7561 gcgtcaccga cgcggcgcgg ccgtcctgcg gcttgatggt gggatggttc atgcggatca
     7621 gctcgttctt gaccttcacc ttcaggcgct tgaacggcat cgcgacgctg aagctgtcct
     7681 tggtgggcag gcccgcgaac ggcggctcgg catgcagcgc gccggcaacc cagccgcgca
     7741 gcgcgtcctc gtcgccggcc atgaacaggt tgatgccctc gtgcgcgagc agcacggtgc
     7801 ccttcaggcc ggccgcggcg gcgctcgcct cgaggcggtc gcgcagcgcg ggcaggtcgt
     7861 ccagcgcgac gaagcggtag gtggagatgt tcaggaactg catgggatgc cggatgggag
     7921 cgcggggccg atacgccgag cgctcgctgt gccgcatttt gccgccgttc cgccgtcttc
     7981 gaaggcggcc gagccaatgg ccgggctgcg gcgtacgtaa gattcggtct ccaagaacat
     8041 gtgggtggga ggaatcatga gaatcgtgcg ggccttggcg tgccttgccg cgaccgcggg
     8101 gtgcacgtcg atccaggtca agccgctgga cgcgtcctcg ccgttgaagc aggtctgcat
     8161 cgtgcagaac ccgcgggtgg aggtctcgga cttcgtcgat gtcctgcgtg acggcttcga
     8221 tcgtcacggc atcgcctcgt cggtggtggc atcggaggat gcgaagccat gcgacgccac
     8281 cttgacgtac acggcgctgc ggacgtggga cctcgcgccg tacctgtccc acgccgaatt
     8341 gcggctgtgg cgcgacggac gccagatcgg ttcggccacc tatcacctca atggcggcgg
     8401 cggcctgtcg ctgatgaaat ggcaaggcac gaagaccaag atggatccgg tgatcgatca
     8461 gctcctggcc gccaagcctt gacgcaaggg tagctgggaa tctcgaattc ctagcacgga
     8521 ttccgggccg aaaagtgcgc attccgcgca gggcggccgt cgcccggctc cctacaatcc
     8581 gggcatggct ttcgttcacc tgcgcaccca taccgagttc tccgtcgtcg acggcacgct
     8641 tcgcatcgac gacgtggcgg ccgcggccaa gggcgacggg caggtggcgc tgggcatcac
     8701 cgacctgtcc aacctgttcg gcgcggtgaa gttctacaag gcctgccgcg gcaaaggcgt
     8761 caagcccatc gtgggcgtcg acgtgtggat ggacccgcgg cccgatgccg ctgatcgcgt
     8821 gcccacgcgc ctcgtgctgc tcgtgcagag ccgccagggc tacctcaacc tctgcgagct
     8881 gctgggccgc agctggacga agaacgtgca gcgcgcgcag gcctgggtgc gctgggactg
     8941 gctcgaggag ctgggcgacg gcatgatcgc gctgtccggc gccgaccacg gcgccgtcgg
     9001 ccaggcgctg ctcgccggcg acgtgccgcg agccgaggcc gcggcgctgg agctcgcgcg
     9061 catcttcccg ggtcgcttct atctcgagct gcagcgcgcc gggctgccca accacgagcc
     9121 ccacgtgcgc gccacggtgc cgctggccgc caggctgcag ctgccggtcg tcgccacgca
     9181 tccggtgcag ttcctcgacc ccgacgactt cgacgcgcac gaggcgcgcg tgtgcatcgc
     9241 cgaaggcgag acgctggcca atccgcgccg cgtcaagcgc ttcacgcgcg accagtactt
     9301 caagggccag gccgagatgg aggcgctgtt cgccgacatc ccgtcggcca tcgccaacac
     9361 cgtggagatc gcgcgccgct gcaacctcac gctcgcgatg ggcaagaact acctgcccga
     9421 ctttcccacg cccatcctgg ccgacggttc ggccatgccg atggacgact acttccgcca
     9481 gctcagcttc gagggcctgg aggatcgcct ggcgctgctg taccccgatg ccgccgtgcg
     9541 cgacgcgaag cgtcccgagt acatcgagcg gctcgagttc gagatcaaga cgatcctgaa
     9601 catgggcttc cccggctact tcctgatcgt gtcggacttc atcaagtggg ccaaggccaa
     9661 cggctgcccg gtgggtccgg gccggggttc gggcgccggc tcgctggtcg cctacgtgct
     9721 caagatcacc gacctcgacc cgctgcagta caagctgctg ttcgagcgct tcctgaaccc
     9781 ggagcgggtg tccatgcccg acttcgacgt cgacttctgc cagggcaacc gcgaccgcgt
     9841 catcgagtac gtgaaggaca agtacggccg cgcggccgtc agccagatcg ccaccttcgg
     9901 cacgatggcc gccaaggcgg cgctgcgcga catcggccgc gtcctgggca tgggctacgg
     9961 ccatgtcgat tccatcgcca agctgatccc cgcgcccccg ggcaagacgg tgacgctggc
    10021 caaggtgccc gagaagcccg atcccggcct gatctacgca cgcctggagg cacccgagct
    10081 cgaccagcgc gagaaggcgg aagaggaagt cgccgaactg ctggcgctgg ccgcgcgcgt
    10141 cgagggcatc gtccgcaaca tcggcatgca cgccggcggc gtgctgatcg cgccgggcaa
    10201 gatcaccgac ttctgcccgc tgtaccagca gccgggcagc gagtccgcgg tgagccagtt
    10261 cgacaaggac gacgtcgagg cgatcggcct ggtcaagttc gacttcctgg ggctggccac
    10321 gctcacgatc ctggaactcg ccaaggattt catcgtggcg cgccgcccgg ggcgcgagaa
    10381 cttctcgtac gagacgctgc cgctggacga tccgaaggtc taccgcctgt tctccgacgg
    10441 cctgaccgag gccgtgttcc agttcgaatc gcgcggcatg cagggcatgc tccgcgacgc
    10501 caagcccagc cgcctcgagg acctgatcgc gctgaacgcg atgttccgcc ccgggccgat
    10561 ggacaacatc ccgtcgttct gcgcgcgcaa gcatggccgc gaggaggtga cctacccgca
    10621 tccgctgctc gagaaggtgc tgggcgagac ctacgggatc atggtctacc agg
//
LOCUS       DATCRO010000259         7569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1015188, whole genome shotgun sequence.
ACCESSION   DATCRO010000259 DATCRO010000000
VERSION     DATCRO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015188"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2343)
                     /locus_tag="VIN75_07060"
     CDS             complement(<1..2343)
                     /locus_tag="VIN75_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8973958.1"
                     /translation="MTDLSAEFKALAAYKPAHKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVIHLGHNRSVDEVVTAALQEDVQGIAVSSYQGGHNEYFAYMVDLLRERGGEHI
                     QVFGGGGGVIVPAEIRALAEKGVRIYSPEDGQRMGLQGMIGEMVMTCDKDISGFAPAR
                     LDELRGHGPQAWRKLAQLITALENKGLDDARVAAVHEAAAGVRTPVIGITGTGGAGKS
                     SLTDELIRRFRLDQRDRLHIAVISIDPSRRKSGGALLGDRIRMNAINPWADGQPKVYM
                     RSLATRDAGSEISQALPDVLAATKLAGFDLIIVETSGIGQGDAAIVPLVDVPMYVMTP
                     EFGAASQLEKIDMLDFAEFVAINKFDRKGAADALRDVAKQVQRNREAFTVMPDKMPVF
                     GTMASRFNDDGVTALYQAMKPRLAQLGLDLGEGALPTVDTRHSTHQTPIVPAARTRYL
                     AEISDTVRGYKKLARVQATLAREVQQLRGAARMLKEADAQKANAHDAVAQLAVEREAR
                     LNAEARKLLAMWPDMQKAYAGDEYVVKIRDKEIRTNLVHTTLSGTKIRKVVLPRFEDH
                     GEILKWLMLDNVPGSFPYTAGVFAFKREGEDPTRMFAGEGDAFRTNRRFKLVSEGMPA
                     KRLSTAFDSVTLYGADPAPRPDIYGKVGNSGVSIATLEDMKVLYSGFDLCSPTTSVSM
                     TINGPAPTILAMFMNTAIDQNLDKFKADNGRQPTADEAAKIKDWVLANVRGTVQADIL
                     KEDQGQNTCIFSTEFSLKVMGDVAEYFVHHNVRNFYSVSIS"
     gene            2533..3312
                     /locus_tag="VIN75_07065"
     CDS             2533..3312
                     /locus_tag="VIN75_07065"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8973959.1"
                     /translation="MSFLAIDIGNTRLKWALFAQPVPGQSPVRHGAVFLETIDDLAES
                     EWADLPPPDQMLGCVVAGEAVRRRVEEQMELWDVVPRWVVPRPAEAGVTNGYDHPSRL
                     GADRWVALVGARARVTAEAAASGRPPRPALVVMVGTAVTIDALDADGRFLGGLILPGF
                     GLMLRALEMGTAGLKVPTGETTEFPTNTSDALMSGGVFAIAGAVERQYKRLAARGGQA
                     PKVLIAGGAAVKLAPALDVEFEIADSLLFEGLVQMEAARGM"
     gene            complement(3443..4033)
                     /locus_tag="VIN75_07070"
     CDS             complement(3443..4033)
                     /locus_tag="VIN75_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8973960.1"
                     /translation="MKKTFIAGAVAVLSLAGSSAFAQAYVGINLGESHANRGCDAGAA
                     SGLIDSCDKTDFAWKINGGYQLPGTPWAGELTYFNLGKFKGTGPTASGTAKDSYWGLG
                     GAYHQDFGSGWGGVARAGAAYGTSKVDYSLAGVSGEQTKNGWHPYYGLGVTYQVAKNI
                     KIEGDWDNTRITSQVPTYASSTAVVNSYTIGASFGF"
     gene            complement(4287..6185)
                     /locus_tag="VIN75_07075"
     CDS             complement(4287..6185)
                     /locus_tag="VIN75_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8973961.1"
                     /translation="MQPRDTDIRATEFDKAEMKAMIERKRRNDYVRKRELDTLRRIRR
                     EGLSGDQAAALTMQSSRMDESVEARSTQSSVQSVGIKAKIDAIERQMVGATSQVSAGG
                     PPPPAAKPARPTPTMPPGVTPPILTTTIFDPEEQTATLPVPLKQDDDHVTVARLDGGP
                     LHLDSPETSPSELGPETAVPTRNFANTMPYAADADPFAPPKASPPPAPIEHAPLEFTP
                     TPFPPPAAPVAAAPTPANVNPYAAGAPAPASRAPANARSADLGGGEKVEVSEVVHDEA
                     LDEAVIAFAGGDFAGCEKALQALTGPRGERRDHDETWLVLFDLYRAIGQQAKFEAITM
                     EYVERFHRSAPQWFSLPKQLAEATKKPAEAGKPKGGIGWVCPPNLDGDAVSRLDALSM
                     QLPSPWVMDWTALSSIDVEGASRLQKLFVGWSKQPLSLEWIAGEKLFSVLQDIAPTGV
                     RDADPVYWMLRLEALRLAHRPDQFDEVAIDYCVTFEVSPPSWENSRCKAQLSGSTQNT
                     QPLSLSVVGEATTSFMDTSMLSEEEAPAQQVQITSLELAGQLSGDLSELLGRLSAQVG
                     DSRVIRISCALLMRVDFVAAGDLLNWVAQRHAEGRELVFHETHRLVALMFGAMGINET
                     ARVQLRHI"
     gene            complement(6320..6973)
                     /gene="dksA"
                     /locus_tag="VIN75_07080"
     CDS             complement(6320..6973)
                     /gene="dksA"
                     /locus_tag="VIN75_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY8973962.1"
                     /translation="MTKTSAKTLAPSTASASGAGAAAPKARAKPATKVSSSADGSAAA
                     GPSLKKPSAKADIEPMNAAKSTATDTKLASAWKSKAGKDLTESELLAMPDSEYMGEKQ
                     LEFFRAKLQALKDDLLSNAGETTEHLREDTSIVPDPADRATIEEEHALELRTRDRERK
                     LLKKISQSISRIDAGDYGYCDETGEPIGLGRLLARPTATLSLEAQQRREMKQKMFGD"
     gene            complement(7150..>7569)
                     /locus_tag="VIN75_07085"
     CDS             complement(7150..>7569)
                     /locus_tag="VIN75_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8973963.1"
                     /translation="EIDPEFLTGQGHDHHHHDHGHEGHDHGPGEACDHPHHHAHDDDV
                     KSFAFKSERAFNPTKLEDFLGAMVQVYGPKMLRYKGVLFMKGSDRKVIFQGVHQMMGS
                     DLGPKWAPGEKKLSKLVFIGIDLPREILLQGLEGCLV"
BASE COUNT         1014 a         2528 c         2706 g         1321 t
ORIGIN      
        1 gctgatcgac accgagtaga agttgcgcac gttgtggtgc acgaagtact cggcgacgtc
       61 gcccatcacc ttcagcgaga actcggtcga gaagatgcag gtgttctggc cctggtcttc
      121 cttgaggatg tcggcctgca cggtgccgcg cacgttggcg agcacccagt ccttgatctt
      181 ggcggcctcg tcggcggtgg gctggcggcc gttgtcggcc ttgaacttgt ccaggttctg
      241 gtcgatggcg gtgttcatga acatcgccag gatcgtcggc gcggggccgt tgatcgtcat
      301 cgacaccgac gtcgtcgggc tgcacaggtc gaagcccgag tacagcacct tcatgtcctc
      361 cagcgtggcg atggacacgc cggagttgcc gaccttgccg tagatgtcgg gccgcggcgc
      421 ggggtctgcg ccgtacagcg tgaccgagtc gaaggccgtg gacagccgct tggccggcat
      481 gccttccgac acgagcttga agcggcggtt cgtgcggaac gcgtcgccct cgccggcgaa
      541 catgcgcgtc gggtcctcgc cctcgcgctt gaacgcgaac acgcccgcgg tgtacgggaa
      601 gctgcccggc acgttgtcca gcatcagcca cttcaggatc tcgccgtggt cctcgaagcg
      661 cggcagcacc accttgcgga tcttggtgcc cgacagcgtc gtgtggacca ggttggtgcg
      721 gatctccttg tcgcggatct tcacgacgta ctcgtcgccc gcgtaggcct tctgcatgtc
      781 gggccacatg gccagcagct tgcgggcctc ggcgttcagg cgcgcctcgc gctcgaccgc
      841 cagctgggcg acggcgtcgt gggcgttggc cttctgggca tcggcctcct tgagcatgcg
      901 ggccgcgccg cgcagctgct gcacctcgcg ggccagcgtc gcctgcacgc gcgcgagctt
      961 cttgtagccg cgcaccgtgt cgctgatctc ggccaggtag cgcgtgcgcg ccgccggcac
     1021 gatgggcgtc tggtgcgtgc tgtggcgcgt gtcgacggta ggcagcgcgc cctcgcccag
     1081 atcgaggccc agctgcgcga ggcgcggctt catcgcctgg tacagcgcgg tgacgccgtc
     1141 gtcgttgaag cggctggcca tcgtgccgaa caccggcatc ttgtcgggca tgacggtgaa
     1201 cgcctcgcgg ttgcgctgca cctgcttggc gacgtcgcgc agcgcgtcgg ccgcgccctt
     1261 gcggtcgaac ttgttgatgg ccacgaactc ggcgaagtcg agcatgtcga tcttctcgag
     1321 ctggctggcg gcgccgaact cgggcgtcat cacgtacatc ggcacgtcga ccagcggcac
     1381 gatcgccgcg tcgccctggc cgatgccgga ggtctcgacg atgatcaggt cgaagcccgc
     1441 gagcttggtg gcggcgagca cgtcgggcag ggcctggctg atctcggagc cggcgtcgcg
     1501 cgtggccagc gaacgcatgt agaccttggg ctggccgtcg gcccacgggt tgatcgcgtt
     1561 catgcggatg cggtcgccca gcagcgcgcc gccgctcttg cgccgcgacg ggtcgatgga
     1621 gatcaccgcg atgtgcaggc ggtcgcgctg gtcgaggcgg aagcggcgga tcagctcgtc
     1681 ggtcagcgac gacttgcctg cgccgccggt gccggtgatg ccgatcaccg gcgtcctgac
     1741 gcccgcggcc gcctcgtgca cggcggcgac gcgcgcgtcg tccagcccct tgttctcgag
     1801 cgccgtgatc agctgcgcca gcttgcgcca ggcctgcggg ccgtggccgc gcagctcgtc
     1861 gagcctggcg ggcgcgaaac cggagatgtc cttgtcgcag gtcatcacca tctcgccgat
     1921 catgccctgc aggcccatgc gctggccgtc ctcggggctg tagatgcgta cgcccttctc
     1981 ggcgagcgcg cggatctcgg ccggcacgat cacgccgccg ccgccgccga acacctggat
     2041 atgctcgccg ccgcgttcgc gcagcaggtc caccatgtag gcgaagtact cgttgtggcc
     2101 gccctggtag ctgctcacgg cgatgccctg cacgtcttcc tgcagcgcgg cggtgaccac
     2161 ctcgtcgacc gagcggttgt ggcccaggtg gatcacctcg gcgcccatgc cctgcaggat
     2221 gcggcgcatg atgttgatgg cggcgtcgtg gccgtcgaac aggctggcgg cggtcacgaa
     2281 gcgcaccttg tgggcgggct tgtacgcggc cagggccttg aattctgcgg agaggtcggt
     2341 catggcaggg gtgtcggtcg ggtttgcgac aaggacgcaa gtatcgggcg aattgacgtt
     2401 tacgtaaacg tcaatttccg ccgggatggc aagccggcgc ggacccgagt gtgaccttcc
     2461 cgacacacct gtgggtttcc actgcccacg accgggtcgc cggcagggag tgggctgata
     2521 cgctcgcgca ctgtgagctt ccttgccatc gacatcggca acacgcgcct gaaatgggcg
     2581 ctgttcgccc agcccgtgcc gggccaatcg cccgtgcgcc acggcgccgt cttcctcgag
     2641 accatcgacg acctggccga gtccgaatgg gccgacctgc cgccgcccga ccagatgctc
     2701 ggctgcgtgg tggccggcga ggccgtgcgc cgccgcgtcg aggagcagat ggaactgtgg
     2761 gacgtcgtcc cgcgctgggt ggtgccgcgc ccggccgagg ccggcgtcac caacggctac
     2821 gaccacccgt cgcgcctggg cgccgaccgc tgggtcgcgc tcgtgggcgc ccgcgcgcgc
     2881 gtgacggccg aggcggctgc gtcgggccgt ccgccgcggc cggcgctggt ggtgatggtc
     2941 ggcacggccg tcaccatcga cgcgctggac gccgacggcc ggttcctggg cggcctgatc
     3001 ctgccgggct tcggcctgat gctgcgggcg ctggagatgg gcaccgccgg cctgaaggtg
     3061 cccaccggcg agaccaccga gttcccgacc aacaccagcg acgcgctgat gtcgggcggc
     3121 gtgttcgcga tcgccggggc cgtcgagcgc cagtacaagc gcctggccgc gcgcggcggc
     3181 caggcgccca aggtgctgat cgccggcggc gccgctgtga agctggcgcc cgccctggac
     3241 gtcgagttcg agatcgccga cagcctgctc ttcgaaggcc tggtccagat ggaagcggcc
     3301 cgaggaatgt gagcccccac gctccctgac ggtcgctgcc ccccgagggg ggctccggtt
     3361 cggctcggat gtgatcctcg cttcccggcc ccttgcgtgg gcctgcggcc ggctgggccg
     3421 caccgcaggt cggctggctt cctcagaagc cgaacgaggc gccgatcgtg tagctgttga
     3481 ccacggccgt cgacgaggcg taggtcggca cctgcgaggt gatgcgggtg ttgtcccagt
     3541 cgccttcgat cttgatgttc ttggccacct ggtaggtcac gcccaggccg tagtacggat
     3601 gccacccgtt cttggtctgc tcgcccgaca cgccagccag gctgtaatcg accttgctgg
     3661 tgccgtaggc cgcgccggca cgggccacgc cgccccagcc gctgccgaag tcctggtgat
     3721 aggcgccgcc caggccccag taggagtcct tggccgtgcc ggaggccgtc gggcccgtgc
     3781 ccttgaactt gccgaggttg aagtaggtca gttcgcccgc ccacggggtg cccgggagct
     3841 ggtagccgcc gttgatcttc caggcgaagt cggtcttgtc gcagctgtcg atcaggccgc
     3901 tggccgcgcc ggcgtcgcag ccgcggttgg cgtggctttc gcccaggttg atgcccacgt
     3961 aggcctgggc gaaggccgaa ctgccggcca gggagagcac ggcgacggcg ccggcgatga
     4021 aagtcttttt cacgatgtgt tccctttggg gtgatgtgct ctcaaggagc gacgatcaaa
     4081 tcctacctag gggtttgccc ttgccggctt aaaaagtcgg tcaggagcaa cacaaaggag
     4141 ttaattcgtg gtaaaggcgt ctttacctcg attccaggga actttctgcg gcgcagggtc
     4201 aatcttgacg ccgacaaaaa aggcgccgga ctcgcgtcgg gcgcctggat tggcggaata
     4261 tcgggagagt gggcgctgac tggatatcag atgtggcgca gctggacgcg cgccgtctcg
     4321 ttgatgccca tggcgccgaa catcagcgcc accagccggt gtgtctcgtg gaacacgagt
     4381 tcgcggcctt cggcatggcg ctgcgccacc cagttgagca ggtcgccggc ggccacgaag
     4441 tccacgcgca tcagcagcgc gcacgagatc cggatgacgc gcgagtcgcc cacctgggcg
     4501 ctgagacggc ccagcagctc ggacaggtcg cccgacagct gcccggccag ctccagcgag
     4561 gtgatctgca cctgctgcgc cggcgcctcc tcctcgctga gcatggaggt gtccatgaag
     4621 ctggtcgtcg cctcgcccac gaccgacagc gacagcggct gcgtgttctg cgtgctgccg
     4681 ctcagctgcg ccttgcagcg cgagttctcc cagctgggcg gggacacctc gaaggtgacg
     4741 cagtagtcga tcgcgacctc gtcgaactgg tcggggcggt gggccaggcg cagcgcctcc
     4801 aggcgcagca tccagtagac ggggtcggcg tcgcgcacgc ccgtgggcgc gatgtcctgc
     4861 agcaccgaga acagcttctc gccggcgatc cactcgagcg acagcggctg cttggaccag
     4921 ccgacgaaca gcttctgcag cctgctggcg ccttcgacgt cgatcgacga cagggccgtc
     4981 cagtccatca cccagggcga gggcagctgc atcgacagcg catccaggcg cgacaccgcg
     5041 tcgccgtcga ggttgggcgg gcagacccag ccgatgccgc ccttgggctt tccggcctcg
     5101 gcgggcttct tggtggcctc ggccagctgc ttgggcagcg agaaccattg cggcgccgag
     5161 cgatggaagc gctcgacgta ctccatcgtg atcgcctcga acttggcttg ctggccgatc
     5221 gcccggtaca ggtcgaacag caccagccag gtctcgtcgt ggtcgcggcg ctcgccgcgc
     5281 gggccggtca gcgcctgcag cgccttctcg cagccggcga agtcgccgcc ggcgaacgcg
     5341 atgacggcct cgtcgagcgc ctcgtcgtgc acgacctcgc tgacctcgac cttctcgccg
     5401 ccgcccaggt cggcgctgcg ggcgttggcc ggggcgcgcg acgccggggc cggggcgccg
     5461 gcggcgtacg ggttgacgtt ggccggcgtc ggcgccgccg ccacgggcgc ggcggggggc
     5521 gggaacggcg tgggggtgaa ctccagcggc gcgtgctcga tcggcgcggg cggcgggctc
     5581 gccttgggcg gcgcgaacgg atccgcgtcg gccgcgtagg gcatcgtgtt ggcgaagttg
     5641 cgcgtcggca cggccgtctc ggggccgagc tccgagggcg aggtctccgg cgagtccaga
     5701 tgcagcggac cgccgtccag gcgcgcgacc gtcacgtggt cgtcgtcctg cttgagcggc
     5761 accggcagcg tggccgtctg ttcctcggga tcgaagatcg tcgtggtcag gatgggcggc
     5821 gtgacgcccg gcggcatcgt cggtgtgggg cgtgccggct tggcggcggg cggcggcggg
     5881 ccgccggccg acacctgcga cgtggcgccc accatctggc gctcgatcgc gtcgatcttg
     5941 gccttgatgc ccacgctctg cacggaggac tgcgtcgagc gcgcctccac gctttcgtcc
     6001 atgcgcgacg actgcatcgt cagcgccgcg gcctgatcgc ccgacaggcc ctcgcggcgg
     6061 atgcgccgca gcgtgtcgag ctcgcgcttg cgcacgtagt cgttgcgccg cttgcgctcg
     6121 atcatggcct tcatctcggc cttgtcgaac tccgtggcgc ggatgtccgt gtcgcgcggc
     6181 tgcatgccga cgagctccct cgcaggcgcc gcgaccttgc gcgccatgcg ccggaacaag
     6241 cctccgccgc cgctttcgtc tttatcggac atggcgcagg gcgctccggc tggagaggga
     6301 agcgggcgtc gggctcaggt cagtctccga acatcttctg cttcatctcg cggcgctgct
     6361 gggcctcgag cgacagcgtg gccgtggggc gcgccagcag gcggcccagg ccgatcggtt
     6421 cgcccgtctc gtcgcagtag ccgtagtcgc ccgcgtcgat gcggctgatc gactgcgaga
     6481 tcttcttgag cagcttgcgc tcgcggtcgc gcgtgcgcag ctcgagggcg tgttcctcct
     6541 cgatcgtggc gcggtcggcc ggatcgggga cgatcgacgt gtcctcgcgc aggtgctcgg
     6601 tggtctcgcc tgcgttggac aggaggtcgt ccttgagcgc ctggagcttc gcgcggaaga
     6661 attccagctg cttctcgccc atgtactcgc tgtcaggcat cgccagcagc tcggattcgg
     6721 tcaggtcctt gcccgccttg gacttccagg cagacgccag cttcgtgtcg gtggcagtgg
     6781 attttgcggc gttcatcggt tctatgtcgg ccttggcgga aggcttcttg agagatgggc
     6841 ccgcggcggc gctgccgtcc gcggacgagg aaaccttggt ggccggcttg gcacgcgcct
     6901 tcggcgcagc ggcgcctgcg ccggatgccg aggcggtgct gggtgccagc gtcttggccg
     6961 aagtcttggt cacggtcggt ctcctcgcga agggtcgctc ggaaggctgt tatacccgca
     7021 gcgagcaaca gtggatagtt gttccaagcg gggctgacct tgcaaaaagg cgggccgatt
     7081 gtagccatgt cccgtcgcga tccgggaaag catttcgccc ggattgcgcg gctttggcaa
     7141 gaaagatgcc tagaccaggc aaccctccag gccctggagc aggatctcgc ggggcaggtc
     7201 gatgccgatg aacaccagct tgctcagctt cttctcgccc ggcgcccact tcgggcccag
     7261 gtcgctgccc atcatctggt gcacgccctg gaagatcacc ttgcggtcgc tgcccttcat
     7321 gaacagcacg cccttgtagc gcagcatctt cgggccgtag acctggacca tcgcgccgag
     7381 gaagtcctcc agcttggtcg ggttgaaggc gcgctcggac ttgaaggcga acgacttcac
     7441 gtcgtcgtcg tgggcgtggt ggtgcgggtg gtcgcaggcc tcgccggggc cgtggtcgtg
     7501 gccctcgtgg ccatggtcgt gatggtggtg gtcgtggccc tggccggtca ggaactcggg
     7561 gtcgatctc
//
LOCUS       DATCRO010000260         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1026602, whole genome shotgun sequence.
ACCESSION   DATCRO010000260 DATCRO010000000
VERSION     DATCRO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026602"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..484
                     /locus_tag="VIN75_07090"
     CDS             14..484
                     /locus_tag="VIN75_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY8973964.1"
                     /translation="MNRLAAILPLANVQVGVDATSKKRVFEQAGVLFENQHAIARATV
                     TDNLFARERLGSTGLGHGVAVPHGRVKGLKNPLAAVLRVQQPIPFDAPDDESVGLLIF
                     LLVPEAATQRHLEILSEIAEMLSDRELRDRLKGDVDAATLHRLISDWEPLKSVA"
     gene            632..1585
                     /gene="hprK"
                     /locus_tag="VIN75_07095"
     CDS             632..1585
                     /gene="hprK"
                     /locus_tag="VIN75_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY8973965.1"
                     /translation="MKPRVISAEALFEEHQPALRWEWVAGHAHPERRFDEAAVREARS
                     AADLIGYLNYIHPYRVQLVGRREVAYLENADQEVQARRIQRIVTLEPPVIIVADGERP
                     PDQLVALCDRAEIPLFVTSASAGHVIDLVRSYLSQLFADRTTRHGVFMDILGVGVLIT
                     GESGLGKSELGLELISRGNGLVADDAVDLYRVSQSTLEGRCPDLLLNLLEVRGIGLLD
                     IKAIFGETAVRRKMRLKLIVHLVRKETMEREFERLPYEPMYDEILGIPVRKVVIAVDA
                     GRNLAVLVEAAVRNTILQLRGIDTYQEFVKRHQDAMAKGGE"
     gene            complement(1746..2201)
                     /gene="fur"
                     /locus_tag="VIN75_07100"
     CDS             complement(1746..2201)
                     /gene="fur"
                     /locus_tag="VIN75_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY8973966.1"
                     /translation="MTHAEELKSSGLKATLPRIKILEVFQQSVERHLTAEDVYKTLLN
                     EGSDIALATVYRVLMQFEQAGILARSHFESGKSVFELNEGTHHDHLLCLTCGRVEEFF
                     DPEIEARQREVAASRGFTLQEHALSLYAVCTRENCEHRPAPKVHPRHHG"
     gene            2308..2871
                     /gene="bamE"
                     /locus_tag="VIN75_07105"
     CDS             2308..2871
                     /gene="bamE"
                     /locus_tag="VIN75_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY8973967.1"
                     /translation="MRITVFSRFLPLSASVLAALALGGCSSFNEPASVKGFLAFIAPY
                     KPDIVQGNVVTTEQMAQVKEGMSKVQVKEILGSPLITDPFHADRWDYVFTLKRQGFDD
                     QRRAFAVMFDKDKVQKIDAPELPSEDEFVAQISRKKLPTSTPKLELTDAEKAALPAPV
                     APAAPAASGVEVAGVTRSYPPLETATP"
     gene            2919..>3450
                     /gene="dapB"
                     /locus_tag="VIN75_07110"
     CDS             2919..>3450
                     /gene="dapB"
                     /locus_tag="VIN75_07110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY8973968.1"
                     /translation="MGRMLIEAVLDAPDCALAGALDVAGSPLLGQDAGAFLGRTTGVA
                     IAADLDAALAASQVLIDFTRPEGTLHHLEACAARGVALVIGTTGFSAEQKAAIGGFAQ
                     RIPVFMAPNMSVGVNVVMRLIEQAAKAFSHGYDIEIIEAHHRNKIDAPSGTALKMGEV
                     VAGALGRNLADNAVFGR"
BASE COUNT          553 a         1234 c         1147 g          516 t
ORIGIN      
        1 cccaggcacg ctcatgaacc gtctcgctgc catccttcct cttgccaacg tccaggtggg
       61 cgtggacgcc accagcaaga agcgcgtgtt cgaacaggcc ggcgtgctgt tcgagaacca
      121 gcacgcgatc gcgcgcgcga cggtcaccga caacctgttc gcccgcgaac gactgggttc
      181 caccggcctg ggccacggcg tcgccgtccc gcacggccgc gtcaagggcc tgaagaaccc
      241 gctggccgcc gtgctgcgcg tccagcagcc catcccgttc gacgcgccgg acgacgagtc
      301 cgtcggcctg ctcatcttcc tgctggtgcc cgaagcggcc acgcagcgcc acctcgagat
      361 cctgtccgag atcgccgaga tgctgtcgga tcgcgagctg cgcgaccgcc tcaagggcga
      421 cgtcgacgcc gccacgctgc atcgactcat ctccgattgg gaaccgctaa agtccgtcgc
      481 gtaagccgca aatgctgatc cccgaacctc tccggcgctg ccgctcgatc ggcccaagcg
      541 gagttcaagg gagcggcgga gccggctttg ccgggccgct cgcggagcac cccctcgggg
      601 ggcagcgagc gcaagcgagc gtgggggcac catgaaaccc cgcgtcatca gtgccgaggc
      661 gctcttcgag gagcaccagc cggcgctgcg ctgggagtgg gtcgcggggc acgcgcaccc
      721 ggaacgccgc ttcgacgagg ccgcggtgcg cgaggcgcgc tcggccgccg acctgatcgg
      781 ctacctcaat tacatccacc cctaccgcgt gcagctggtg ggccgccgcg aggtcgcgta
      841 cctcgagaac gccgaccagg aagtgcaggc gcgccgcatc cagcgcatcg tgacgctgga
      901 gcccccggtc atcatcgtgg ccgacggcga gcgtccgccc gaccagctcg tcgcgctgtg
      961 cgaccgcgcc gagatcccgc tgttcgtgac gtcggcctcg gccggccacg tcatcgacct
     1021 ggtgcgctcg tacctgagcc agctgttcgc cgaccgcacc acgcgccacg gcgtgttcat
     1081 ggacatcctg ggcgtgggcg tgctgatcac cggcgagtcc gggctcggca agagcgaact
     1141 cggcctggag ctgatctcgc gcggcaacgg cctggtcgcc gacgacgccg tcgatctcta
     1201 ccgcgtctcg cagtccacgc tcgaagggcg ctgtcccgac ctgctgctga acctgctgga
     1261 agtgcgcggc atcggcctgc tcgacatcaa ggcgatcttc ggcgagaccg ccgtgcgccg
     1321 gaagatgcgc ctgaagctca tcgtgcacct cgtgcgcaag gagacgatgg agcgcgagtt
     1381 cgagcgcctg ccctacgagc cgatgtacga cgagatcctg ggcatccccg tgcgcaaggt
     1441 cgtcatcgcc gtcgatgccg gccgcaacct cgccgtgctg gtggaggccg ccgtgcgcaa
     1501 cacgatcctg cagctgcgcg ggatcgatac ctaccaggaa ttcgtcaagc gccaccagga
     1561 cgcgatggcc aaaggcggcg agtagcccgg cgaggtcgcc aaccacaaca acgccgtcat
     1621 cctggcgcga agtcgcagga tccacgctgg cgagcagcgc tgcctcaagg ttgaggtgga
     1681 ttccgcgact gcgcgcggaa tgacgggtcg tgcgtgccga gtctcgcgcg acgacgacgc
     1741 cgccctcagc cgtgatgccg cggatggacc ttcggcgccg gccgatgctc gcagttctcg
     1801 cgcgtgcaca ccgcgtacag cgacagcgca tgctcctgca gcgtgaagcc gcgcgacgcg
     1861 gccacctcgc gctggcgcgc ctcgatctcg ggatcgaaga actcctcgac gcggccgcag
     1921 gtcaggcaca gcaggtggtc gtggtgggtg ccctcgttga gctcgaacac cgacttgccg
     1981 gactcgaaat ggctgcgcgc gaggatgccg gcctgctcga actgcatgag cacgcggtag
     2041 acggtggcga gcgcgatgtc agacccttca ttaagcaggg tcttgtagac gtcctcggcc
     2101 gtcaggtggc gttcgacgga ctgctggaag acctcgagga tcttgatccg cggcaaggtc
     2161 gccttcaggc cgctgctctt gagttcttca gcgtgggtca tgttaagcag gacaaagtcg
     2221 cgcttcgcgg gacgcggcgc agactagaat caccggtcaa tcatatccac ccttggcctc
     2281 gttggggcgg cggttcagag cttgtcgatg cgcatcacgg tcttctcccg gttccttccc
     2341 ctgtccgcct ccgtcctggc cgctctcgca ctcggcggct gctccagctt caacgagccg
     2401 gcgtccgtca agggcttcct cgccttcatc gcgccgtaca agcccgacat cgtccagggc
     2461 aacgtcgtca cgaccgagca gatggcgcag gtcaaggaag gcatgtccaa ggtgcaggtc
     2521 aaggagatcc tcggctcgcc gctgatcacc gacccgttcc acgccgaccg ctgggactac
     2581 gtcttcacgc tcaagcgcca gggcttcgac gaccagcggc gcgcgttcgc cgtgatgttc
     2641 gacaaggaca aggtgcagaa gatcgacgcg ccggaactgc ccagcgagga cgagttcgtc
     2701 gcccagatca gccgcaagaa gctgcccacg tccacgccca agctcgagct gaccgacgcc
     2761 gagaaggccg cgctgcccgc gccggtggcc ccggccgcgc ccgcggcgtc gggtgtcgag
     2821 gtggccggcg tcacgcgcag ctatccgccg ctcgagacgg ccacgccgtg agcgccgcgg
     2881 ccccgcttcg catcgcgatc gccggcgcct ccgggcgcat gggccgcatg ctgatcgaag
     2941 ccgtgctgga cgcaccggac tgcgcgctcg ccggcgcgct cgatgtcgca ggcagcccgc
     3001 tgctgggcca ggacgccggc gcgttcctcg gccgcaccac gggcgtggcc atcgccgccg
     3061 acctcgacgc cgcgctcgcc gccagccagg tgctgatcga cttcacgcgc cccgagggca
     3121 cgctgcacca cctggaggcc tgcgccgcgc gcggcgtcgc gctggtgatc ggcaccaccg
     3181 gtttcagcgc cgagcagaag gccgcgatcg gcggcttcgc ccagcgcatc cccgtgttca
     3241 tggcgcccaa catgagcgtg ggcgtgaacg tcgtgatgcg cctgatcgag caggccgcca
     3301 aggcgttctc gcacggctac gacatcgaga tcatcgaggc ccaccaccgc aacaagatcg
     3361 acgcgcccag cggcaccgcg ctgaagatgg gcgaggtcgt ggcgggcgcc ctggggcgca
     3421 acctggccga taacgcggtg ttcggacgcg
//
LOCUS       DATCRO010000261         7374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_419417, whole genome shotgun sequence.
ACCESSION   DATCRO010000261 DATCRO010000000
VERSION     DATCRO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419417"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VIN75_07115"
     CDS             <1..666
                     /locus_tag="VIN75_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8973969.1"
                     /translation="WLRAHGAAVHLRKRVTALARDEGRWRVDCAAAGDDSCKPEPPFD
                     QVILATPATDAARLTHVLAPAWSAVAAALAHEPIVTITVTAPPRAWPAPMLALDANDV
                     DAPAQFAFKLGEQPAGADRVTLVVSAAGRWLARGSDAVAAAAIGQYRRAFGIAERAPI
                     DLSSVRADKRATFRCTAGVLRPGMDIAPGLRAAGDWVAGPYPATLESAVRAGEAAARA
                     SVG"
     gene            747..1520
                     /locus_tag="VIN75_07120"
     CDS             747..1520
                     /locus_tag="VIN75_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8973970.1"
                     /translation="MSKSEGSGPTIQVLARAYALLDVLAAHTDPVPLKELSARTGLHP
                     STAHRILNDLAEGRFVERPEAGSYRLGMRLLELGNLVKARLDVREAAIGPMRELHKLT
                     HQPVNLSVRQGDEIVYIERTYSERSGMQVVRAVGGRAPLHLTSVGKLFLANDEPQRVR
                     AYATRTGLTGHTRNSLTDLPMLERELDKIRRTSISRDDEELELGVRCMAAGIYDDQGR
                     LVAGLSISAPADRLEETWLDRVKETAAKISAALGHNVAA"
     gene            complement(1935..2960)
                     /gene="pbpG"
                     /locus_tag="VIN75_07125"
     CDS             complement(1935..2960)
                     /gene="pbpG"
                     /locus_tag="VIN75_07125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY8973971.1"
                     /translation="MTCAVGSALVCASFAASATVTESSTTATPHHRKAATHAVRGKRV
                     VSVRRTTTVTRTTTRISEVPSQSVGYAAGLHSTEDPLLLRSGAALVLDQDTKEVLYSK
                     NPEAVLPIASLSKLMTALVISEAHQPLDEELTITTDDIDTEKNTRSRLKPGTTLTRGV
                     MLHLALMASENRAANALGRNYPGGLTAFVAAMNAKAKELGMNDTHYVEPTGLSSQNKS
                     SARDLAVLVNAAHQVPLLREYSTTPSLDVELGRRQVQFHTTDRLVANPTWDIGLQKTG
                     FINEAGQCLVMQAQMAGRKLIMVLLDSAGKYSRIADAERIRKWVMANHGASQTPSPTT
                     RTADATS"
     gene            complement(3467..5164)
                     /gene="leuA"
                     /locus_tag="VIN75_07130"
     CDS             complement(3467..5164)
                     /gene="leuA"
                     /locus_tag="VIN75_07130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8973972.1"
                     /translation="MLKNPATKYRPVAPVRLADRTWPDVVLTKAPVWCSSDLRDGNQA
                     LIEPMDGERKMRMFETLVRTGFKEIEIGFPSASQTDFDFVRKLIEENLVPDDVTIQVL
                     TPAREHLIRRTFEAVVGAKKCIMHLYNATAPIMRKVVLGVDVEGVIELAASHARLIKE
                     LAARQPGTDWRFEYSPEMFSDTELDVSRRVIDAVTEVWQPTPERTCIINLPTTVEHST
                     PNIYADMIEWTCRNIQRRDAIVISAHPHNDRGTGTATAELAMMAGADRIEGCLFGNGE
                     RTGNVDIVNIALNLYTQGVDPQLDFSDIDEIRRVVEYCNQLPVHPRHPYVGDLVFTSF
                     SGSHQDAIKKAFAARREGDVWEMPYLPIDPKDLGRSYEAIIRVNSQSGKGGISYLLES
                     EYGLELPRRLQIEFSQVVQAVMDAQGREVTASDIWRIFESEYRLAEGAITMSDLTQTD
                     VGSGRVRVVGHAAVTGSRIAFEGEGNGPVDAFVDGLSRAAGVPVKVLNYHEHSMGGGA
                     QARAAAYVEVRVGEAAPVFGVGIDPNIVRATLLAVLSATSRGASTLVVEEGAAQAVA"
     gene            complement(5432..6067)
                     /locus_tag="VIN75_07135"
     CDS             complement(5432..6067)
                     /locus_tag="VIN75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8973973.1"
                     /translation="MIDLATLATFMAVVLGLFLVPGPAVLLVLTRTAQGGRRVGIATG
                     AGIALGDLVHTLLAAVGLSALLLTSALAFGIVKLCGAGYLLWLGVRAFRERPVAGESS
                     PASSVPAVSASRAFWQAIPAEVLNPKTALFFLAFMPQFVHAGRGHALAQFLVLGLVFV
                     AMSLVYSTTLAFVARRLGQAVGRLRWLDRWKGRLIGAIFVSLGVRVAFQQQ"
     gene            complement(6152..6988)
                     /gene="pssA"
                     /locus_tag="VIN75_07140"
     CDS             complement(6152..6988)
                     /gene="pssA"
                     /locus_tag="VIN75_07140"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY8973974.1"
                     /translation="MNDLIPTPDDEVDDDVHVAPRRRRKGVYILPNLFTLAALFGGFY
                     AIVMAMNGHYVLSCYGIFCAMVLDSLDGRVARMTNTQSAFGAEMDSLSDMVSFGAAPA
                     LIVYEWALKGLGKAGWGIAFFYCACAALRLARFNTNLAVVDKRYFQGLPSPAAAALVV
                     GFIWVVVDTSSGDVNGDMVLSWAAAIATFYAGVTMVVNVPFYSFKDLSLKRSVPFIVI
                     VLIALAIGIISLQPARMIFAIFCLYGVSGYFVYAYKRAKGRPVSAIALSTDEPDEVGL
                     HR"
     gene            complement(7224..>7374)
                     /locus_tag="VIN75_07145"
     CDS             complement(7224..>7374)
                     /locus_tag="VIN75_07145"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY8973975.1"
                     /translation="FILENRAGAPTLLSRRRLNAEHPIEVVGEQLRAMMPWIKANKLV
                     DKSRN"
BASE COUNT         1208 a         2499 c         2525 g         1142 t
ORIGIN      
        1 tggctgcgcg cgcacggcgc ggccgtccat ttgcgcaaac gcgtcaccgc gctcgcacgc
       61 gacgagggcc gctggcgcgt cgattgcgca gcggccggcg acgactcgtg caagccggag
      121 ccgccgttcg atcaagtgat cctcgccacg cccgcaaccg acgccgcgcg cctgacgcac
      181 gtgctggcgc ccgcctggtc agccgtcgcg gccgcgctcg cgcacgagcc catcgtcacc
      241 atcacggtga ccgcgccgcc gcgcgcgtgg cctgcgccca tgctggcgct cgacgcgaac
      301 gacgtcgacg cgcccgcgca gttcgcgttc aagctgggcg agcaaccggc cggcgccgac
      361 cgcgtcacgc tggtggtgag cgccgccgga cgctggctgg cgcgcggcag cgacgcggtc
      421 gccgcggcgg ccatcgggca gtaccggcgc gcgttcggca tcgccgagcg cgcgccgatc
      481 gacttgtcga gcgtgcgcgc cgacaagcgc gccacgttcc gctgcaccgc cggcgtgctg
      541 cggcccggca tggacatcgc gcccggcctg cgcgccgccg gcgactgggt ggccggcccc
      601 taccccgcca cgctggagtc ggccgtccgt gccggcgaag cggccgcccg cgcatcggtc
      661 ggttagagcc ggctgtcgtg actcgcagcg tgaatccggg tttcaccatg cgagaataag
      721 catcccacag cccaggccga ccatccatgt ccaagtcaga aggctccgga cccaccatcc
      781 aggtcctcgc acgcgcctac gcgctgctcg acgtcctggc cgcgcacacc gaccccgtgc
      841 cgctgaagga gctcagcgcc cgcaccgggc tgcacccgtc caccgcccac cgcatcctca
      901 acgatctggc cgaaggtcgc ttcgtcgaac gccccgaggc cggcagctac cgcctgggca
      961 tgcgcctgct ggagctcggc aacctcgtga aggcgcggct ggacgtgcgc gaggccgcca
     1021 tcggcccgat gcgcgaactg cacaagctga cgcaccagcc ggtcaacctg tcggtgcgcc
     1081 agggcgacga gatcgtctac atcgagcgca cctacagcga gcgctcgggc atgcaggtcg
     1141 tgcgcgccgt cggcggccgc gcgccgctgc acctcacgtc ggtcggcaag ctgttcctgg
     1201 ccaacgacga accccagcgc gtgcgcgcct acgcgacgcg cacgggcctg accggccaca
     1261 cgcgcaacag cctcaccgac ctgccgatgc tcgaacgcga gctcgacaag atccggcgca
     1321 cctcgatctc gcgcgacgac gaggagctcg agctcggcgt gcgctgcatg gccgccggca
     1381 tctacgacga ccagggccgc ctcgtggccg ggctgtcgat ctcggcgccg gccgatcggc
     1441 tcgaggagac ctggctggat cgcgtgaagg aaaccgcggc gaagatctcg gcggcgctgg
     1501 ggcataacgt cgcggcttga ggaggcgttc gcattccgca aggcgaaggg ccgcattccg
     1561 cggctcttct tgtctgggtc cgcacctcgt cgcgggatgg cgagccacgt tgccgcgtct
     1621 gcaggaagcg tgtcatcctg cgcgcagtcg caggacccac cccgccctgg aggtggccgt
     1681 tccgggacgc cgcaggcctt ggccgacgtg gcttccgcga ctccgcttcg ctccgcgcag
     1741 aatgacggct cgacgccgcg caaattgaaa gggcagcctc gcggcagctc cttttatttg
     1801 ctccgcaaat tcaaagggca gcctcgcggc agctcctttt atttgctctg caaattgaaa
     1861 gggccggcag cttgcgccac cggccctttt cccatcgcgt tgttgttctg gcgatcgagg
     1921 agaaaccttg cgggtcagct cgtggcgtcg gccgtgcggg tcgtcgggga cggcgtctgc
     1981 gatgcgccgt gattggccat gacccacttg cggatgcgct cggcatcggc gatgcgcgag
     2041 tacttgccgg ccgaatcgag cagcaccatg atcagcttgc ggccggccat ctgggcctgc
     2101 atcaccaggc actggccggc ttcgttgatg aagccggtct tctgcaggcc gatgtcccag
     2161 gtcggattgg cgaccaggcg gtcggtggtg tggaactgca cctggcggcg gcccagttcg
     2221 acgtccagcg acggcgtcgt cgagtattcg cgcagcagcg gcacctggtg ggccgcgttc
     2281 accagcacgg cgaggtcgcg cgcgctggac ttgttctggc tcgacaggcc ggtgggctcg
     2341 acgtagtgcg tgtcgttcat gcccagttcc ttggccttgg cgttcatcgc cgcgacgaag
     2401 gccgtcaggc cgcccgggta gttgcggcca agggcattgg cggcgcggtt ctccgacgcc
     2461 atcagcgcga ggtgcagcat cacgccgcgc gtcagcgtgg tgccgggctt gaggcgcgat
     2521 cgcgtgttct tctcggtgtc gatgtcgtcc gtcgtgatcg tcagctcttc gtcgagcggc
     2581 tggtgcgcct cgctgatcac cagcgcggtc atcagcttgc tcagcgaggc gatcggcagc
     2641 acggcctccg gattcttgct gtacaggact tccttggtgt cctggtccag caccagcgcc
     2701 gcgcccgaac gcagcagcag cggatcctcg gtggaatgca ggccggcggc gtagccgacc
     2761 gactgcgacg gcacttcgct gatgcgggtg gtcgtgcgcg tgacggtggt cgtgcggcgc
     2821 accgagacga cacgcttgcc gcgcacggcg tgcgtcgccg ccttgcgatg gtgcggcgtg
     2881 gccgtggtgc tgctctcggt gacggtcgcc gacgcggcga acgaggcaca aaccagggcg
     2941 ctgccgaccg cgcaggtcaa ggcccaacgc gacagttgct tgaatttccg cgaaacggaa
     3001 gagctggggg ccagttgcat caccatttgt ttcagtctaa agcaaaggaa aaatccatgc
     3061 aagatcaaaa gcttgcgtgg aattcctcaa ccggacgtcg aacgagcgta ccagctgtcg
     3121 accggcctag ggatggtcag accgccggat aaagctgtct aacgcaggaa accgggtttc
     3181 ggctgacgca gcgacgaaaa atctgggagt cggatccacg ccgggacgga acgttcacat
     3241 tgaagaaaac gcaatgccat cctcaggatc ggattttccg catccaccat gagccggatg
     3301 gtccgaaaag cgggcatttg ccacggtaat ccgggccggc acgtgactta aggtaacgcg
     3361 aggcccgtcc gcgtttccca acgcaacggg cctctactga ctttcccgga tccgggacgc
     3421 tcgcgcgatc ccggagcccg caggcgggcc gggatcgcgg gcaggatcag gcgacggcct
     3481 gcgccgcgcc ttcttcgacc accagcgtgc tggcgccgcg cgacgtggcc gacagcaccg
     3541 ccagcaaggt ggcgcgaacg atgttggggt cgatgccaac gccgaacacg ggcgccgcct
     3601 cgcccacacg cacctcgacg taggccgccg cgcgcgcctg cgcgccgccg cccatcgagt
     3661 gctcgtggta gttcagcacc ttgacgggca cgcccgcggc gcgcgacagg ccgtcgacga
     3721 acgcgtcgac cgggccgttg ccctcgcctt cgaacgcgat gcggctgccg gtcaccgcgg
     3781 catgccccac gacgcgcacg cggcccgagc cgacgtccgt ctgcgtcagg tcggacatcg
     3841 tgatggcgcc ttcggccaac cggtattcgg attcgaagat tcgccaaatg tccgacgccg
     3901 tgacttcgcg cccctgcgca tccatgacgg cctgcacgac ctggctgaac tcgatttgga
     3961 ggcggcgcgg cagctcgagc ccgtactccg actcgagcag gtacgagatg ccgcccttgc
     4021 cggactggct gttgacgcgg atgatcgcct cgtagctgcg gcccaggtcc ttcgggtcga
     4081 tcggcaggta cggcatctcc cagacgtcgc cctcgcggcg cgcggcgaac gccttcttga
     4141 tggcgtcctg gtgcgagccc gagaacgacg tgaagacgag gtcgcccacg tacgggtggc
     4201 gcgggtgcac cggcagctgg ttgcagtact cgacgacgcg gcggatctcg tcgatgtcgg
     4261 agaagtccag ctgcgggtcg acgccctgcg tgtacaggtt cagcgcgatg ttgacgatgt
     4321 cgacgttgcc cgtgcgctcg ccgttgccga acaggcagcc ctcgatgcgg tcggcgccgg
     4381 ccatcatcgc cagctcggcg gtggcggtgc cggtgccgcg gtcgttgtgc ggatgcgcgg
     4441 agatcacgat ggcgtcgcgg cgctggatgt tgcggcacgt ccactcgatc atgtcggcgt
     4501 agatgttggg cgtggaatgc tccaccgtcg tgggcaggtt gatgatgcag gtgcgctcgg
     4561 gcgtgggctg ccacacctcg gtgacggcat cgatcacgcg gcgcgagacg tcgagctcgg
     4621 tgtccgagaa catctcgggc gaatactcga agcgccagtc ggtgccgggc tgcctggcgg
     4681 ccagttcctt gatgagacgc gcatggctgg cggccagctc gatgacgccc tcgacgtcca
     4741 cgccgagcac gaccttgcgc atgatcggcg cggtggcgtt gtacagatgc atgatgcact
     4801 tcttggcgcc cacgacggcc tcgaacgtgc ggcggatcag gtgctcgcgt gcgggcgtca
     4861 gcacctggat cgtgacatcg tcgggcacca ggttttcctc gatcagcttg cgcacgaaat
     4921 cgaagtccgt ctgcgacgcc gacggaaagc cgatctcgat ctccttgaag ccggtgcgca
     4981 ccagcgtctc gaacatgcgc atcttgcgct cgccgtccat cggctcgatc agcgcctggt
     5041 tgccgtcacg caggtcggaa ctgcaccaca cgggggcctt ggtcaggacg acgtcgggcc
     5101 aggtgcggtc ggccaggcgc acgggggcga cgggacggta cttcgtggcg gggttcttga
     5161 gcatggacat cgctggttct ccaggaggga tttttaggat ggcaacgccg cactggaaat
     5221 gaaaaacggc ccgctgcgtt gcgcaaggcg ggccgttgat cggaatttcg gttcagacgt
     5281 gactgatcaa tgcacccgcg gtactccggc tagggatagc gtgatcagcg caattcggga
     5341 cgtcatgccg acgaatatag cacggccttt ccggcttgtg gcgaccaatt cttcgcgttg
     5401 gacgaagcaa tccttcgcaa ggacgaatct ttcactgctg ctggaacgcg acgcgcacgc
     5461 ccagcgacac gaagatcgcg ccgatcagcc tgcccttcca gcggtcgagc catcgcaggc
     5521 ggccgacggc ctggcccagg cggcgggcga cgaaggccag ggtcgtcgaa tagaccaggc
     5581 tcatggcgac gaataccagg cccagcacga ggaactgcgc cagcgcgtgg ccgcgcccgg
     5641 catggacgaa ctgcggcatg aacgcgagga agaacaacgc cgtcttcgga ttgagcacct
     5701 cggcggggat cgcctgccag aaggcgcgcg acgccgatac cgccggcacc gaactcgccg
     5761 gcgacgactc gccagcgacg ggacgctccc ggaacgcccg cacgcccagc cacagcaggt
     5821 agcccgcgcc gcacagcttg acgatgccga acgccagcgc cgaggtcagc agcagcgccg
     5881 acagcccgac cgccgccagc agcgtgtgca cgaggtcgcc cagggcgata cccgcgccgg
     5941 tggcgatgcc cacgcggcgc ccgccctggg cggtgcgcgt cagcaccagc aggacggccg
     6001 ggcccggcac caggaacagc ccgaggacga cggccatgaa ggtggccagg gtagcgaggt
     6061 cgatcacggc agtcccgtcg gcgagtgagg tcgtcgtgga ttgaaccaga tcgcgcccgg
     6121 acgcgcacgc gcccgcgcgt gacgcgaggc ctcagcgatg caggccgacc tcgtcgggct
     6181 cgtcggtgga cagcgcgatg gcgctgaccg gccggccctt ggcgcgcttg tacgcataga
     6241 cgaaatagcc cgagacgccg tacaggcaga agatcgcgaa gatcatgcgc gccggctgca
     6301 ggctgatgat gccgatcgcc agcgcgatca gcacgatcac gatgaagggc accgaacgct
     6361 tcaggctgag gtccttgaag ctgtagaacg gcacgttcac gaccatcgtg acgccggcgt
     6421 agaacgtggc gatggccgcg gcccaggaca gcaccatgtc gccgttgacg tcgccgctgg
     6481 aggtgtcgac caccacccag atgaagccca ccaccagcgc ggcggccgcc ggactgggca
     6541 ggccctggaa gtagcgcttg tccaccaccg cgaggttggt gttgaagcgc gccaggcgca
     6601 gcgccgcgca ggcgcagtag aagaacgcga tgccccagcc cgccttgccc agtcccttga
     6661 gcgcccattc gtagacgatc agcgccggcg cggcaccgaa gctgaccatg tcggacaggc
     6721 tgtccatctc ggcgccgaac gcgctctgcg tgttggtcat gcgggcgacg cggccgtcca
     6781 ggctgtccag caccatcgcg cagaagatgc cgtagcacga cagcacgtag tgcccgttca
     6841 tcgccatcac gatggcgtag aagccgccga acagcgcggc cagcgtgaac aggttgggca
     6901 ggatgtagac gcccttgcgg cgccggcgcg gcgccacatg cacgtcgtca tcgacctcgt
     6961 cgtccggagt cgggatcagg tcgttcatcg cgcacgcatc gggaaaaaat tcatggtgcg
     7021 ggaggatacc gcagcgccgg ccgcggcccg tgtgtcgata ccaaagaaaa aggccaccga
     7081 agtggccttg ggacctgccc ggcgccgccg ctcattcgac tcgaacgaga gtcgagggag
     7141 cggcggatcc ggcttcgccg ggccgctcgc gtccggcccc tcgggggcag ggagcgcgag
     7201 cggaccgtgg ggtcgtcagt tcttcagttc ctggacttgt cgaccagctt gttggccttg
     7261 atccacggca tcatcgcgcg cagctgctcg cccaccacct cgatcgggtg ctcggcgttc
     7321 aggcggcggc gcgacagcag cgtcggggcg ccggcgcggt tctccaggat gaag
//
LOCUS       DATCRO010000262        12577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_295502, whole genome shotgun sequence.
ACCESSION   DATCRO010000262 DATCRO010000000
VERSION     DATCRO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295502"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIN75_07150"
     CDS             complement(<1..252)
                     /locus_tag="VIN75_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973976.1"
                     /translation="MDPLDIELDRLFAVASGATPMTLADAAGRTRALVLEVVQPTGWD
                     ALAPLWQGVQAELGLPAPAIAVSGTDGLQLWFSTESPVDP"
     gene            complement(257..1210)
                     /locus_tag="VIN75_07155"
     CDS             complement(257..1210)
                     /locus_tag="VIN75_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HEY8973977.1"
                     /translation="MTTPHDTAPYPIGTPGTPWGPQERAAWLARQRIRRSYETEVVQP
                     LKARLPAAAHLFSYGVLDYARLGLPAYPLLAVRSRDWQAGRPTVLVTGGVHGYETSGV
                     QGALQWIADDFERHARDVNVLVLPCISPWGYETVNRWNPDALDPNRNFRQDSPAAESA
                     LAMACVAAHGGAVDLHVDLHETTDTDNSEFGPAKAARDGKAFDWHAIPDGFYLVGDTE
                     RPAPDFQRALVEAVRPVTHIADADEHGCIIGTKLEQPGVINYAKRELGLCGGMTAARF
                     VTTTEVYPDSPAATPAQCNAAQVATVVAAIDYLLDDGAAAR"
     gene            1414..2325
                     /gene="ylqF"
                     /locus_tag="VIN75_07160"
     CDS             1414..2325
                     /gene="ylqF"
                     /locus_tag="VIN75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEY8973978.1"
                     /translation="MAIQWFPGHMHTTRKAIAERLPDIDVVIELLDARLPGSSANSLL
                     AEMTQGKPALKVLNKQDLADPELTDAWLAHYRAQPGTRAIGLDASVAGPAKALVTECR
                     ALAPNRGGMVKPVRVLICGIPNVGKSTLVNTLNGQRAAKTGDEPGITKMEQKIVLAND
                     FYLFDTPGMLWPRILIEEGGYNLAASGAIGRNAYDEELVAMALLSRVRHRYADRITTR
                     YKTADFTAMTDEELLAEVGRRRGGLMAGGRVNMQKAAEIALNDFRSGAWGRVTLETPD
                     EFAAWWAAGMAAEAQRLEKKAASRKKR"
     gene            complement(2335..2622)
                     /locus_tag="VIN75_07165"
     CDS             complement(2335..2622)
                     /locus_tag="VIN75_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEY8973979.1"
                     /translation="MTDAASLPTLPDRLSVDPRSPHHVAAIFDHDIGIRFNGKERFDV
                     EEYCISEGWVRVPAGAKKVDRKGNPLMIKLKGTVEAFVKPAPAAAPDAPAA"
     gene            complement(2671..3075)
                     /locus_tag="VIN75_07170"
     CDS             complement(2671..3075)
                     /locus_tag="VIN75_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY8973980.1"
                     /translation="MKTLPLRLSPGDDLRAALADAAAAAGAEAAFVLAGIGSLREARL
                     RLAGRDALERLAGDLEILTLSGTLGAGGAHLHASLSDADGRVLGGHVAPGCIVRTTAE
                     VLLALLDDVRFTREPDAATGYAELHVWPRGGA"
     gene            3138..3464
                     /locus_tag="VIN75_07175"
     CDS             3138..3464
                     /locus_tag="VIN75_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8973981.1"
                     /translation="MPTEPDDVFKALADPTRRALLERLLREGRELNVRALTAQAGVSQ
                     PMVSKHLNVLKAAGLVQERPDGRVVHYRAEPKGLAPLMDWMRFYAVFWDERMDRLENL
                     LNRMDQ"
     gene            3464..3913
                     /locus_tag="VIN75_07180"
     CDS             3464..3913
                     /locus_tag="VIN75_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8973982.1"
                     /translation="MNTATASATTRSVVVERRMPHSQDKIWRALTQGPLLEDWLMSND
                     FEPRVGHRFTFRMPPMEGWNGVTDCEVLSVEPPNELSYTWNSSGEEAANGLKTVVKWT
                     LEPVDGGVLVRMEQSGFREQDQRNFVGARYGWERNLGELERVVASLA"
     gene            3910..4506
                     /locus_tag="VIN75_07185"
     CDS             3910..4506
                     /locus_tag="VIN75_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8973983.1"
                     /translation="MSAHAPTTGRAGPGARAGARANKYHPVLVLLHWTLAWFIIGSLL
                     FGFFVLRTLPNSDPRKIDMLEWHMGLGMAILAAMLIRLAVRWRTARPAPAGLGTPASA
                     RAAGRAHIAFYVLVAGMVASGYATALIAHLNDIVFARNGAPLPPSFDPYPTFHLHGWL
                     AALLAVLVTLHVLGALWHWLVRRDGVMRRMGWGGRRVE"
     gene            4586..5485
                     /locus_tag="VIN75_07190"
     CDS             4586..5485
                     /locus_tag="VIN75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY8973984.1"
                     /translation="MTIRRTGHRYRDLATGKTLDVWFPRDPANARRGCLAEKLGLVTG
                     DVVQVQIESLDAAPASTEDVWLRLHLLSERAVKPNELNLEGVFALLNNIAWTSAGPVL
                     PARVEELRALAAAEFHHLAVPSVDKFPRMSDYVVPEGVRVADADRVRLGAHLAAGTTV
                     MHEGFVNFNAGTLGESMVEGRVTPGVVVGRNSDVGAGASIMGTLSGGGKTKNSIGERS
                     LLGANAGIGISLGDECIVEAGLYVTAGTKVKLPDGRVVAARTLSGRPGLLFRRNSQSG
                     AVEVLETDAQRWGGLNTALHSND"
     gene            5541..6005
                     /locus_tag="VIN75_07195"
     CDS             5541..6005
                     /locus_tag="VIN75_07195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8973985.1"
                     /translation="MKAFQDHYAADVAHCYGCGALNTHGHRIKTHWDGDGTVTRFTPL
                     PFHTAVPGFVYGGLIASLVDCHSTGSAFAAMVRSQGRELDAQPAPRFVTGSLQVSYLK
                     PTPLGVELELRGTIKEIKGRKVIVETTVLAGGVATATGTVVAVQMPDNFGAP"
     gene            6186..6854
                     /locus_tag="VIN75_07200"
     CDS             6186..6854
                     /locus_tag="VIN75_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY8973986.1"
                     /translation="MDLPGFTDRWQTERQQLFKSVPPEGRIVRYLDKEFLVYPDTFWP
                     FADSLPLVTHLRIEPGDSVLDVGTGSGVIGIFACHGGASRVVGVDINPAAIRSATHNA
                     RMHGFAGTMEVRQSDLFEALGDERFDVVTANLPFRNKPAHDVVAMSQWDTGFRTNTRF
                     FEGVGRHLRPGGRIYFAQSNFGEIEAALRLARSAGLQVKELARESIDDTRRQEFLVYE
                     MTMA"
     gene            6858..8096
                     /locus_tag="VIN75_07205"
     CDS             6858..8096
                     /locus_tag="VIN75_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973987.1"
                     /translation="MDSRLSTIKARRGVPVTELGPAVRRVVVVLCGSRGGSSLFKEAL
                     AQHPQVASLDGELDPLLALSGNGFNHNSDSDCLEAVANASELASSILDEISLPSPEIL
                     ETALLQRKWRNRLALQFPSHFLDPQAYDRLEQSLQAALSRIDRADRRNEADLQRLILA
                     SVFSEEPWRIGYYDGNAALGDSRHFDEPLKIEEPPFVMPTLRRRPFTPSDARDAVLLF
                     KSPSDAYRIGMYERLFPNAEVTYIHLTRGYAQSVNGLMDGWLYPLGFFSHDLSKVGCE
                     LAIRGYSDQVSFGRKWWKFDLPRNWRRFTQATLEEVCLNQWYSIHAAILRRGVCSLRV
                     SFEDFVASPASVLRRVTDHLGLPAMALPGALPVKMATDQPRPGRWHKRAAMLIPLSAR
                     PDVRTMMAMLGYSMDEATWL"
     gene            8087..8917
                     /locus_tag="VIN75_07210"
     CDS             8087..8917
                     /locus_tag="VIN75_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY8973988.1"
                     /translation="MAVNARLLSPYLMGQRRDGLRRVRDVDHHPWELLTLHPQPAARI
                     DDPIEKLRNLGDLYAMLAARVAALVRSGCRPVSLAGDCVSTLGMLAGLQQAGQPPDRV
                     LWLDAHGDFHTWSSTQTQYIGGMPLAMFVGRVDDGPDPRSQATRACLSAIGAQAYPPQ
                     RVVLADARDLDPGEREALLGSAILRCRLGDVLAQLRPHERLYVHWDTDVIDDPARMPA
                     LKYHVRSGPSAADIATLFRALRGHQVVAVSVSAWHEEQDGDNRTARACLDVLDALLDA
                     "
     gene            complement(8940..10520)
                     /locus_tag="VIN75_07215"
     CDS             complement(8940..10520)
                     /locus_tag="VIN75_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973989.1"
                     /translation="MTVMNERTIRILSVEDSENDFRLAMRQLRKAGFAPEGRRVQDLP
                     SLRQALQEGPWDAMISDFSMPGFSGLEALSEFRTTGLDIPFIFVSGTIGEEMAVAAMR
                     AGASDYVMKENMGRLGPALERELAQVAQRAAHRQAEAELVRFEAQLHQAQKMESLGTL
                     AGGIAHDFNNILGAILGNIEIALADIEPGHPAQPALGEIQQASLRARDLVRQILTFSR
                     RRPQQLEVVALKPIVEETFRLLRATLPAGVDLRQDLAQAPMYVHADATQLQQVVMNLC
                     TNAWHALHDKAGLILIGLDAAEVDADDGLARGLDPGAYAHLWVKDTGSGMDARTLERV
                     FEPFFTTKSTGQGTGLGLSVVHGIVAAHHGSITVDTLPGRGSTFHVLLPATSEPLPLP
                     ASHERAAMVAAHGEHVVYLDDDETVMLVMVRILERAGYRCSGYLDPAAALASIQAAPA
                     TVDVFITDYNMPGRSGLDVAREAAALKPGMPIAISTGLITDELREQARALGVVAVIEK
                     ENAFREIVPLVRRLVDKD"
     gene            complement(10517..10960)
                     /locus_tag="VIN75_07220"
     CDS             complement(10517..10960)
                     /locus_tag="VIN75_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8973990.1"
                     /translation="MNEESKTILLVEDNDDDVALTLRAFKRSHLMNPVTVVRDGVEAL
                     DFLFQRGAYAGRSEGLPTLVILDLKLPKLDGLGVLKAMRADARTSLLPTVILTSSREE
                     QDVIAGYSLGANSYVRKPVDFAEFLEAVKVLGIYWLALNQVAPPQ"
     gene            complement(10926..12395)
                     /locus_tag="VIN75_07225"
     CDS             complement(10926..12395)
                     /locus_tag="VIN75_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8973991.1"
                     /translation="MKIRTKATFAAAMMLGTMVLIGASIGWADAQVRAAVAQRRHSAD
                     IASALNNMRMVTFEFLLNRRDRARIQEQVVWERLERLFATPVALDDSAAGVLASLRSQ
                     GEQGHRLFNEVEAASGAGATDDVQRRFEAQLSSRIMILQQNSLVQAEHLIQDAEKRID
                     AAQRRVVLVTGLGLLVMALLTAATAWLFRRDVLRPIARLELATREVAAGNWSFELDVG
                     STDELGDVARHFDAMTRALRDSFNQLQVSNRDLVVLNKELESFSYSVSHDLRSPLRSM
                     DGFSLALLEDYGDRLDEEARDSLQRIRAASQRMGRLIDELLGLARVTRAELRIQEVDL
                     SAMAEEILSTLAKAEPERQVRWEIDGGMRVLGDRELVAIALQNLLANAWKFTSKVAQA
                     VIRVGMRDEDGKQVCFVSDNGAGFDMAYASRLFGAFQRLHHERDFPGTGVGLATVQRV
                     MHRHGWSLGASAALGQGATFYFRPTEQVDERREQDHPAG"
     gene            complement(12392..>12577)
                     /locus_tag="VIN75_07230"
     CDS             complement(12392..>12577)
                     /locus_tag="VIN75_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973992.1"
                     /translation="AWQDVTLEVEQQQAQLVTLEDVATWAMTRHYAPSQPVPNFFSHL
                     ELETMLAVSPGRVTVVR"
BASE COUNT         1819 a         4430 c         4471 g         1857 t
ORIGIN      
        1 cggatcgacg ggagactccg tcgagaacca cagttgcagg ccgtcggtgc ccgacacggc
       61 gatggccggc gccggcaggc cgagctcggc ctgcacgcct tgccaaagcg gcgccagcgc
      121 gtcccagccc gtgggctgaa ccacctccag cacgagggcg cgcgtgcgac ccgcggcgtc
      181 ggcgagcgtc atcggcgtcg cgcctgaggc gacggcgaag agccgatcga gttcgatgtc
      241 cagagggtcc atggcgtcac cgcgcggccg cgccgtcgtc gagcaggtag tcgatcgcgg
      301 cgaccaccgt ggccacctgc gccgcgttgc attgcgcggg cgtggcggcg gggctgtcgg
      361 ggtagacctc ggtcgtcgtc acgaagcggg cggcggtcat gccgccgcac aggcccagtt
      421 cgcgcttggc gtagttgatg acgccgggct gctcgagctt cgttccgatg atgcagccgt
      481 gctcgtcggc gtcggcgatg tgggtcacgg gacgtacggc ctcgaccagc gcgcgctgga
      541 aatcgggcgc cgggcgctcg gtgtcgccga ccaggtagaa gccgtcgggg atcgcgtgcc
      601 agtcgaaggc cttgccgtcg cgcgcggcct tggccgggcc gaactcgctg ttgtcggtgt
      661 cggtggtctc gtgcaggtcg acgtgcaggt cgaccgcgcc gccgtgcgcc gccacgcacg
      721 ccatcgccag ggccgattcg gcggccgggc tgtcctggcg gaagttgcgg ttcgggtcga
      781 gcgcgtcggg gttccagcgg ttgacggtct cgtagcccca cgggctgatg cagggcagca
      841 ccagcacgtt gacgtcgcgc gcgtggcgct cgaagtcgtc ggcgatccat tgcagcgcgc
      901 cctgcacgcc gctggtctcg tagccgtgca cgccgccggt caccagcacg gtcggcctgc
      961 cggcttgcca gtcgcggctg cgcacggcaa gcagcggata ggcgggcagg cccaggcgcg
     1021 cgtaatcgag cacgccgtac gagaacaggt gcgccgccgc gggcaggcgc gccttgagcg
     1081 gctggacgac ctcggtctcg tagctgcgcc ggatgcgctg gcgcgcgagc caggcggccc
     1141 gttcctgcgg gccccagggc gtgccggggg tgccgatggg gtagggcgcg gtgtcgtgtg
     1201 gcgtggtcaa ggaatcgtcc cggagatggc gatcccggac gatagcgcaa cgccgggtcg
     1261 cgtgacatgc cggcgaccgg ccgaccgccc gcgcggggga cgggcgaccc ggcggtcatc
     1321 ctggcgggtc ggacgacctg gcccgcgagc ggccacgctt cgcgcatgac ggaaaatgcc
     1381 gcctgttcct tcgtcccaga aagcttcctc cagatggcca tccagtggtt cccgggtcac
     1441 atgcacacga cccgcaaggc gatcgccgag cgcctgccgg acatcgacgt cgtcatcgaa
     1501 ctgctcgacg cccgcctgcc cggctccagc gcgaactcgc tgctggccga gatgacgcag
     1561 ggcaagccgg cgctcaaggt gctgaacaag caggacctgg ccgaccccga gctcaccgac
     1621 gcctggctcg cgcactaccg cgcgcagcct ggcacgcggg ccatcgggct cgatgcgagc
     1681 gtcgccgggc cggccaaggc gctcgtcacc gaatgccgcg cgctcgcgcc caaccgcggc
     1741 ggcatggtca agccggtgcg cgtgctgatc tgcggcatcc ccaacgtggg caagtccacg
     1801 ctcgtgaaca cgctcaacgg ccagcgcgcg gccaagacgg gcgacgagcc gggcatcacg
     1861 aagatggagc agaagatcgt cctggccaac gacttctacc tgttcgacac ccccggcatg
     1921 ctgtggccgc gcatcctgat cgaggagggc ggctacaacc tggccgccag cggcgccatc
     1981 ggccgcaacg cgtacgacga ggagctcgtg gcgatggcgc tgctgtcgcg cgtgcgccat
     2041 cgctacgccg accgcatcac cacgcgctac aagacggccg acttcacggc gatgaccgac
     2101 gaggagctgc tggccgaggt gggccgccgg cgcggcggcc tgatggccgg cgggcgcgtg
     2161 aacatgcaga aggccgcgga gatcgcgctc aacgacttcc gcagcggcgc ctgggggcgc
     2221 gtcacgctgg agacgccgga cgaattcgcg gcgtggtggg cagccgggat ggccgccgag
     2281 gcgcagcggc tcgagaagaa ggccgcgtcg cgcaagaagc gctgatcgcg caggtcaggc
     2341 ggcgggcgcg tccggcgccg cggcgggcgc gggcttcacg aaggcctcga cggtgccctt
     2401 gagcttgatc atcagcgggt tgcccttgcg gtcgaccttc ttggcgccgg cgggcacgcg
     2461 cacccagcct tcgctgatgc agtactcctc gacgtcgaag cgctccttgc cgttgaagcg
     2521 gatgccgatg tcgtggtcga agatcgcggc cacgtgatgc gggctgcgcg ggtcgacgga
     2581 caggcggtcg ggcagcgtgg gcagggaggc ggcgtcggtc atggcgggag aactctggcg
     2641 ggaaacccgt cattctagat cgcgaggacc tcacgccccg ccgcgcggcc agacgtgcag
     2701 ctcggcgtag ccggtggccg catcgggctc gcgcgtgaag cgcacgtcgt ccagcagcgc
     2761 cagcagcacc tcggccgtgg tgcgcacgat gcagcccggc gcgacgtggc cgcccagcac
     2821 gcggccgtcg gcgtccgaca ggctcgcgtg caggtgcgca ccgcccgcgc ccagcgtgcc
     2881 cgacagcgtg aggatctcga ggtcgccggc gaggcgctcg agcgcgtcgc gtccggccag
     2941 ccgcagacgg gcttcgcgca ggctgccgat gcccgcgagc acgaacgcgg cctcggcgcc
     3001 ggcggcggcg gccgcgtcgg ccagggcggc gcgcaggtcg tcgccgggcg agagtcggag
     3061 tggcagcgtc ttcatgattg ccagcgtagc gcatggggcc tttgacgttt aaataaccat
     3121 atggttatga ttcgtccatg cccaccgaac cggatgacgt cttcaaggcc ctggccgacc
     3181 ccacgcggcg cgcgctcctc gagcgcctgc tgcgcgaggg ccgcgagctc aacgtccgcg
     3241 cgctcaccgc gcaggcgggt gtctcgcagc cgatggtctc caagcatctg aacgtgctga
     3301 aggccgctgg gctggtgcag gaaaggccgg acggccgcgt cgtgcactac cgcgccgagc
     3361 ccaagggcct ggccccattg atggactgga tgcgcttcta cgccgtcttc tgggacgagc
     3421 gcatggatcg cctcgaaaat ttgctcaaca ggatggacca atgatgaaca ccgcaactgc
     3481 ctccgccacc acccgcagcg tcgtcgtcga gcgtcgcatg ccgcattcgc aggacaagat
     3541 ctggcgcgcc ctcacgcagg ggccgctgct ggaggactgg ctgatgtcca acgacttcga
     3601 gccgcgcgtg ggccaccgct tcaccttccg catgccgccg atggagggct ggaacggcgt
     3661 gaccgactgc gaggtgctgt cggtcgagcc gccgaacgaa ctgtcgtaca cgtggaactc
     3721 gtcgggcgag gaggccgcca acggcctgaa gaccgtcgtc aagtggacgc tggagccggt
     3781 cgacggcggc gtgctggtgc gcatggagca gtcgggcttc cgcgagcagg accagcgcaa
     3841 cttcgtgggc gccaggtacg gctgggaacg caacctgggc gaactggagc gcgtcgtcgc
     3901 gagcctggca tgagcgcgca cgcaccgacc acgggccggg ccggccccgg ggcgcgggcc
     3961 ggcgcccgcg ccaacaagta ccaccccgtg ctggtgctgc tgcattggac gctggcgtgg
     4021 ttcatcatcg gctcgctgct gttcgggttc ttcgtgctgc gcacgctgcc caactccgat
     4081 ccgcgcaaga tcgacatgct cgagtggcac atgggcctgg gcatggcgat cctggcggcg
     4141 atgctgatcc gcctggccgt gcgctggcgc acggccaggc ccgcgcccgc gggcctgggc
     4201 acgcccgcgt ccgcgcgcgc ggcgggccgg gcgcacatcg cgttctacgt gctggtggcg
     4261 ggcatggtgg ccagcggcta tgcgacggcg ctcatcgcgc acctgaacga catcgtcttc
     4321 gcgcgcaacg gcgcgccgct gccgccgtcg ttcgacccgt accccacgtt ccacctgcat
     4381 ggctggctcg cggcgctgct ggccgtcctc gtcacgctgc acgtgctggg ggcgctgtgg
     4441 cactggctgg tgcgccgcga cggcgtgatg cggcgcatgg gctggggcgg gcggcgcgtc
     4501 gaatagaggg ttgccgcacg gcgaaccggg cggcccgcgg tgcgtggcat cctgcggggt
     4561 cccgacgcct tgcacgaccc gcatcatgac catccgacgc accggccacc gctaccgcga
     4621 cctcgccacc ggcaagaccc tggacgtctg gttcccgcgc gacccggcca acgctcgccg
     4681 cggctgcctg gccgagaagc tggggctcgt caccggcgac gtcgtccagg tgcagatcga
     4741 atcgctggac gcggcgcccg cgtcgaccga ggacgtgtgg ctgcggctgc acctgctgtc
     4801 ggagcgtgcg gtcaagccca acgagctcaa cctcgagggc gtgttcgcgc tactgaacaa
     4861 catcgcgtgg acgtccgccg gccccgtgct gccggcgcgc gtcgaggaac tgcgcgcgct
     4921 cgcggccgcc gagttccacc acctggcggt gccgtccgtc gacaagttcc cgcgcatgag
     4981 cgactacgtg gtgcccgagg gcgtgcgcgt ggccgacgcc gaccgcgtgc gcctgggcgc
     5041 gcacctggcg gctggcacca ccgtcatgca cgagggcttc gtcaacttca acgcaggcac
     5101 gctgggcgag tcgatggtcg agggccgcgt cacgccgggc gtggtcgtcg gccgcaactc
     5161 cgacgtcggc gcgggcgcgt ccatcatggg cacgctgtcg ggcggcggca agacgaagaa
     5221 ttccatcggc gagcgcagcc tgctgggcgc caacgcaggc atcgggatct cgctgggcga
     5281 cgagtgcatc gtcgaggcgg gcctgtacgt gaccgccggc accaaggtca agctgcccga
     5341 cggccgcgtg gtggccgcgc gcacgctctc cggccgcccg ggcctgctgt tccgccgcaa
     5401 cagccagagc ggcgccgtcg aggtgctgga gaccgacgcg cagcgctggg gcgggctcaa
     5461 cacggcgctg cattcgaacg actgaaggct tgctacagtg cctcggcgcg gccacgagcc
     5521 gcgaaccatc gaggtcgccc atgaaagcct tccaggacca ctacgccgcg gacgtcgcgc
     5581 actgctacgg ctgcggcgcg ctcaacacgc acggccaccg catcaagacg cactgggacg
     5641 gcgacgggac ggtcacgcgc ttcacgccgc tgcccttcca cacggcggtg cccggcttcg
     5701 tctacggcgg cctgatcgcc tcgctggtgg actgccacag caccggcagc gccttcgcgg
     5761 cgatggtccg ctctcagggc cgggagctcg atgcgcagcc ggcgccgcgc ttcgtcaccg
     5821 gctcgctgca ggtcagctac ctcaagccga cgccgctggg cgtcgagctg gagctgcgcg
     5881 gcacgatcaa ggagatcaag ggccgcaagg tgatcgtcga gacgacggtg ctggcgggcg
     5941 gcgtggccac cgcgacgggc acggtggtgg cggtgcagat gccggacaac ttcggggcgc
     6001 cctgaaagcg ccgtggcgca ccgggcgctg cccgggcgtg ccgtcgaacg ccgccgcata
     6061 ccccgtgggg ctccgctcgg ccatgccccc gcgtcgccat gatgagatac acccaggggc
     6121 ataccacgat cgcgctgcgt ggcgggccgg cctatcctcg ctcgacagac tccgaggagt
     6181 gatcaatgga cttgcctggc ttcaccgatc gctggcagac cgagcggcag caactcttca
     6241 agtccgtccc tcccgagggc cggatcgtcc gctacctgga caaggagttc ctagtctacc
     6301 cggacacctt ctggccgttc gccgacagcc tgccgctggt cacgcatttg cggatcgaac
     6361 cgggtgacag cgtgctggac gtgggcacgg gctcgggcgt catcggcatc ttcgcctgcc
     6421 acggcggcgc gtcgcgggtg gtgggcgtgg acatcaatcc ggccgccatc cgcagcgcga
     6481 cgcacaacgc ccggatgcac ggctttgccg gcaccatgga agtccggcag tcggacctgt
     6541 tcgaggcgct gggagacgag cgtttcgacg tcgtcacggc caacctgcca ttccgcaaca
     6601 agcccgcgca cgacgtggtg gccatgtccc agtgggacac cggctttcgc accaacacgc
     6661 gcttcttcga aggggtcgga cggcacctgc gtcccggcgg ccgcatctac ttcgcgcagt
     6721 ccaacttcgg cgagatcgag gcggccctgc gcctggcgcg ttcggccggc ctgcaggtca
     6781 aggaactggc ccgcgagtcg atcgacgaca cgcggcgcca ggaattcctg gtctacgaga
     6841 tgaccatggc ctaggcgatg gacagcaggc tgtcgacgat caaggcgcgt cgcggtgtcc
     6901 ccgtcaccga gctggggccc gccgtccggc gggtggtggt cgtgctgtgc ggctcgcggg
     6961 gcggctccag cctgttcaag gaagcgctgg cccagcatcc gcaggtggcc tcgctagacg
     7021 gcgagctcga tccgctgctg gcgctgtcgg gcaacggctt caaccacaat tccgacagcg
     7081 actgcctcga ggccgtggcc aacgcgtccg aactggccag cagcatcctg gacgagatct
     7141 cgctgccctc gccggagatc ctcgagacgg ccttgctgca gaggaaatgg cggaaccggc
     7201 tcgcgctgca gttcccgtcg catttcctgg atccgcaggc ctacgaccgg ctcgagcagt
     7261 cgctgcaggc cgcgttgagc cggatcgacc gcgcggaccg ccgcaacgag gccgacctgc
     7321 agcgcctgat cctcgcatcg gtcttcagcg aggaaccctg gcgcatcggc tactacgacg
     7381 gcaacgcggc gctcggcgac agccggcatt tcgacgaacc gctcaagatc gaggagccgc
     7441 cgttcgtgat gcccacgctc cggcgccgcc ccttcacgcc gtcggacgcg cgcgacgccg
     7501 tgctgctgtt caagtcaccc tccgacgcct accgcatcgg catgtacgag cgcctgttcc
     7561 ccaacgccga agtcacctac atccacctca cccggggcta cgcgcagtcg gtgaacggcc
     7621 tgatggacgg gtggctgtat ccgctgggct tcttctcgca cgacctgagc aaggtcgggt
     7681 gcgagctcgc catccggggc tattcggacc aggtgtcgtt cggccgcaag tggtggaagt
     7741 tcgacctgcc gcgcaactgg cggcgtttca cccaggcgac gctggaagag gtctgcctga
     7801 accagtggta ctccatccac gcggcgatcc tgcggcgcgg ggtctgctcg cttcgggtgt
     7861 ccttcgagga cttcgtcgcc agtcccgcgt cggtgctcag gcgggtgacg gatcacctgg
     7921 gcctgcccgc catggcgctg cccggcgcgc tgcccgtcaa gatggcgacg gaccagccgc
     7981 gacccggccg ctggcacaag cgagcggcca tgctcatccc gctgagcgcg cggcccgacg
     8041 tgcgcaccat gatggccatg ctcggctatt cgatggacga agcgacatgg ctgtgaacgc
     8101 gcgactcctg tcgccctacc tgatgggcca gcgccgcgac ggcctgcgcc gcgtgcgcga
     8161 tgtcgaccac catccgtggg agctgctgac gctccatccg cagccggccg cgcggatcga
     8221 cgaccccatc gagaagctgc gcaacctggg cgacctgtac gcgatgctgg ccgcgcgcgt
     8281 cgcggcgctg gtccgcagcg gctgcaggcc ggtcagcctc gccggcgatt gcgtgtcgac
     8341 gctgggcatg ctggcggggc tgcaacaggc cggccagccg cccgatcgcg tgctgtggct
     8401 ggacgcccac ggcgacttcc atacctggtc gagcacgcag acccagtaca tcggcggcat
     8461 gcccctggcc atgttcgtcg ggcgcgtcga cgacgggccg gacccgcgca gccaggcgac
     8521 ccgcgcgtgc ctgtcggcca tcggcgcgca ggcgtatccg ccgcagcggg tcgtcctcgc
     8581 cgacgcgcgc gacctggacc cgggcgagcg cgaggcgctg ctgggctcgg ccatcctgcg
     8641 ctgccggctc ggcgacgtgc tggcgcagct gcggccgcac gagcgcctct acgtgcactg
     8701 ggacaccgac gtcatcgacg acccggcccg catgcccgcg ctgaagtacc acgtccgcag
     8761 cggcccgagc gccgcggaca tcgcgacgct cttccgcgcc ctgcggggcc accaggtcgt
     8821 cgccgtgtcc gtctcggcct ggcatgagga gcaggacggc gacaaccgca ccgcccgcgc
     8881 ctgcctggac gtcctggacg cgctgctgga cgcctgacgc ctcgacaggc ccggcgacac
     8941 tagtccttgt ccaccaggcg ccgcacgagc gggacgatct cgcggaacgc gttctccttc
     9001 tcgatgacgg ccaccacgcc cagggcgcgc gcctgttcgc ggagctcgtc cgtgatgagc
     9061 cccgtgctga tcgcgatggg catgcccggc ttcagggcgg ccgcctcgcg ggcgacgtcc
     9121 agacccgagc gtccgggcat gttgtagtcg gtgatgaaga catccaccgt cgcgggcgcg
     9181 gcctggatgg atgccagcgc cgcggcggga tccaggtagc cgctgcagcg gtagccggcg
     9241 cgctcgagga tgcgcaccat caccagcatc accgtctcgt cgtcatcgag gtagacgacg
     9301 tgctcgccat gcgcggccac catcgcggcg cgctcgtggc tggccggcag gggcagcggc
     9361 tcggacgtcg ccggcagcag cacgtggaag gtgctgccgc ggcccggcag ggtgtcgacg
     9421 gtgatgctgc cgtggtgcgc cgcgacgatc ccgtgcacca cggacaggcc caggcccgtg
     9481 ccctggcccg tcgacttggt cgtgaagaac ggctcgaaga cacgctcgag cgtgcgggcg
     9541 tccatgccgg agccggtgtc cttgacccac aggtgcgcat aggcgcccgg gtcgaggccg
     9601 cgcgccaggc cgtcgtcggc gtcgacttcg gcggcgtcca ggccgatgag gatcaggccg
     9661 gccttgtcgt ggagcgcgtg ccaggcgttg gtgcacaggt tcatcaccac ctgctgcagc
     9721 tgcgtggcgt cggcgtgcac gtacatgggc gcctgggcca ggtcctgccg caggtcgacc
     9781 cccgcgggca gcgtcgcgcg caggaggcgg aacgtctcct cgacgatcgg cttgagcgcg
     9841 acgacctcca gctgctgcgg acggcggcgg ctgaaggtca ggatctgccg cacgaggtcg
     9901 cgggcgcgca ggctggcctg ctggatctcg cccagggcgg gctgcgccgg atggccgggc
     9961 tcgatgtcgg ccagcgcgat ctcgatgttt cccaggatcg cgccgaggat gttgttgaag
    10021 tcgtgcgcga tcccgccggc cagcgtgccc agggactcca tcttctgggc ctggtgcagc
    10081 tgcgcctcga agcggaccag ctcggcctcc gcctggcggt gcgccgcgcg ctgcgcgacc
    10141 tgcgccagct cgcgctcgag cgccgggccc aggcgcccca tgttctcctt catgacgtag
    10201 tcgctggcgc cggcccgcat cgcggcgacg gccatctcct cgccgatcgt tccggagacg
    10261 aagatgaagg ggatgtccag ccccgtggtg cggaactcgg acagcgcttc caggccgctg
    10321 aagccgggca tgctgaagtc ggagatcatc gcgtcccacg gtccctcctg cagcgcctgc
    10381 cgcagcgagg gcaggtcctg cacgcgccgt ccctccggcg cgaagcccgc cttgcgcagc
    10441 tgccgcatcg cgaggcggaa gtcgttctcg gagtcctcga ccgagaggat cctgatcgtg
    10501 cgctcgttca tgacggtcat tgcggaggcg caacctggtt gagcgcgagc cagtagatgc
    10561 ccagcacctt gacggcttcc aggaactcgg cgaagtccac cggcttgcgc acatagctgt
    10621 tggcgcccag cgaatagccg gcgatgacgt cctgctcctc tctcgacgac gtcaggatga
    10681 cggtcggcag gagcgacgtg cgcgcgtcgg cgcgcatggc cttgagcacg cccaggccgt
    10741 cgagcttggg cagcttcagg tcgaggatca ccagcgtcgg caagccctcg ctcctgcccg
    10801 cgtaggcgcc gcgctggaac aggaagtcga gcgcctcgac gccgtcgcgc accacggtga
    10861 ccgggttcat caggtggctg cgcttgaagg cgcgcagcgt caacgcgacg tcgtcgtcgt
    10921 tgtcctcaac cagcaggatg gtcttgctct cttcgttcat cgacttgctc cgtgggtcgg
    10981 aaatagaagg tcgcgccctg gccgagggcg gcggacgcgc cgagcgacca gccgtggcga
    11041 tgcatcacgc gctgcacggt ggccaggccg acgcccgtgc cagggaagtc gcgctcgtga
    11101 tgcaggcgct ggaacgcgcc gaagagacgg gaggcgtagg ccatgtcgaa gccggcgccg
    11161 ttgtccgaga cgaagcacac ctgcttgccg tcctcgtcgc gcatgcccac gcggatgacg
    11221 gcctgggcga ccttgctcgt gaacttccag gcgttggcga gcaggttctg cagcgcgatc
    11281 gcgaccagct cgcggtcgcc caggacccgc atccccccgt cgatctccca gcggacctgt
    11341 cgctcgggtt cggccttggc cagggtcgag aggatctcct cggccatcgc gctgaggtcg
    11401 acctcctgga tccgcagctc ggcgcgggtg acccgcgcga ggcccagcag ctcgtcgatc
    11461 aggcgaccca tccgctggct ggccgcgcgg atgcgctgca ggctgtcgcg ggcctcctcg
    11521 tcgaggcggt cgccgtaatc ctccagcaat gccagggaga agccgtccat gctgcgcagc
    11581 gggctgcgca ggtcgtgcga gacggaatag ctgaacgact ccagttcctt gttcagcacc
    11641 acgaggtcgc ggttgctgac ctgcagctgg ttgaacgaat cgcgcagcgc ccgcgtcatc
    11701 gcgtcgaagt ggcgcgccac gtcccccagc tcgtcggtgc tgcccacgtc gagctcgaac
    11761 gaccagttgc cggcggccac ctcgcgcgtg gccagctcca ggcgggcgat cggccggagg
    11821 acgtcgcgcc ggaacagcca cgccgtcgcg gcggtcagca gggccatcac cagcaggccc
    11881 agccccgtga cgaggacgac acgccgctgg gcggcgtcga tccgcttctc cgcatcctgg
    11941 atcaggtgct cggcctgcac gaggctgttc tgctgcagga tcatgatccg gctggacagc
    12001 tgcgcctcga agcgccgctg cacgtcgtcc gtggcgcccg cgccggacgc tgcctcgacc
    12061 tcgttgaaca ggcgatgtcc ctgctcgccc tggctgcgca ggctcgcgag caccccggcc
    12121 gccgaatcgt cgagcgccac cggcgtggcg aacaggcgct cgagccgctc ccacacgacc
    12181 tgctcctgga tccgcgcccg gtcgcgccgg ttcagcagga actcgaaggt caccatgcgc
    12241 atgttgttga gcgcgctggc gatgtccgcg ctgtggcgcc gctgggcgac cgcggcgcgc
    12301 acctgggcgt cggcccagcc gatgctcgcg ccgatcagca ccatcgtgcc gagcatcatc
    12361 gccgccgcga acgtggcctt ggtacggatc ttcatcggac caccgtcacg cgtccggggc
    12421 tgaccgccag catcgtctcg agctcgaggt gcgagaagaa gttgggcacc ggctggctgg
    12481 gcgcgtagtg gcgtgtcatc gcccaggtcg ccacgtcctc cagcgtgacc agttgcgcct
    12541 gctgctgctc cacttcgagc gtcacgtcct gccaggc
//
LOCUS       DATCRO010000263         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_621692, whole genome shotgun sequence.
ACCESSION   DATCRO010000263 DATCRO010000000
VERSION     DATCRO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621692"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..455)
                     /locus_tag="VIN75_07235"
     CDS             complement(36..455)
                     /locus_tag="VIN75_07235"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8973993.1"
                     /translation="MTHAELQASSKAPAALPADKELVLRVLPMPADSNANGDIFGGWI
                     MAQVDLAGSVLPARIAKGRITTVAVNQFIFKQPVSIGDLLSFYATVERVGNTSITVRV
                     EVYAERNPADVQVVKVTEANLTYVAIDRDGKPRQIPR"
     gene            632..>2514
                     /locus_tag="VIN75_07240"
     CDS             632..>2514
                     /locus_tag="VIN75_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8973994.1"
                     /translation="MHPNPPSAARDAAPATEPRSDLATLKRLMPYFWEYRVRMAVALA
                     FLVGAKVASVSVPILLKKLVDALNISVSDPRALLVVPLGLLLGYAGLRLAMTLFTELR
                     EIVFARATFGAAKRVALETFEHLNALSLRFHLERQTGGLTRDIERGTRALQTLVSYSL
                     YTIIPTLVELTFVLVLLGHRFDMGYVWITLAALLVYGTYTISITEWRTKYRKAVNELD
                     SKANTKAIDALINFETVKYFNNEQFESARYETGLEAYRAAQMKSQATLSMLNTGQQLI
                     IAGSLVALLWRATGGVIAHSMTLGDFVMVNALLIQLYVPLNFLGVLYREVKQGLTDLE
                     RMFALLEKEREIADAPDAVPLRVDGGAVRFEHVSFAYEPARPILHDVSFEIPAGKTVA
                     VVGPSGAGKSTLARLLYRFYDVTGGAIRIDGQDLRQVTQHSLRKAIGIVPQDTVLFND
                     TVAYNIAYGRPGASQAEVEGAARAAHIHDFIAATPKGYETMVGERGLKLSGGEKQRVA
                     IARTLLKNPPLMIFDEATSALDSANERAIQAELAQAAQGRTTLVIAHRLSTVVNAAQI
                     LVMAEGRIVERGTHPELLAMNGRYAHMWRLQQTHGDTAAATDAPGSPTLGDSAFAAAA
                     PAR"
BASE COUNT          385 a          898 c          841 g          390 t
ORIGIN      
        1 cgcctgcggc tgacccgccc taccgcagat tccgctcagc gcggaatctg ccgcggcttc
       61 ccgtcgcggt cgatcgccac gtaggtcagg ttcgcctcgg tcaccttgac cacctgcacg
      121 tcagccgggt tgcgctcggc gtagacctcc acgcgcaccg tgatcgacgt gttgcccacg
      181 cgctcgaccg tggcgtagaa gctgagcagg tcgccgatcg agacgggctg cttgaagatg
      241 aactggttca ccgcgaccgt cgtgatgcgc cccttggcga tgcgcgccgg cagcaccgag
      301 ccggccaggt cgacctgggc catgatccag ccgccgaaga tgtcgccgtt cgcgttggaa
      361 tcggccggca tcggcagaac gcgcagcacc agctccttgt cggcgggcag cgcggcgggc
      421 gccttcgagg aggcttgcaa ttcggcgtgg gtcatcgggg gctttctgcg gtgtcgggct
      481 cgacgggcat tgtccatcaa agggtttacc ctgtgttttc gattgtgccg cgcatccatc
      541 gacggtcgac cctaaactgc cccgccggcg ccgacacgcg gactttgttc acgcttgacc
      601 tggcgcccgc cgcccccacc tgcgcagtcc catgcacccg aatccccctt ccgccgcccg
      661 cgacgccgcg cccgccaccg aaccgcgcag cgacctggcc acgctcaagc ggctgatgcc
      721 gtatttctgg gaataccgtg tgcgcatggc ggtcgcgctc gctttcctgg tcggcgccaa
      781 ggtggccagc gtcagcgtgc cgatcctgct caagaagctg gtcgacgcgc tcaacatctc
      841 ggtgagcgac ccgcgcgcgc tgctggtggt gccgctcggc ctgctgctgg gctacgccgg
      901 cctgcgcctg gcgatgacgc tgttcaccga gctgcgcgag atcgtgttcg cgcgcgccac
      961 cttcggcgcg gccaagcgcg tcgcgctgga gaccttcgag cacctgaacg cgctgtccct
     1021 gcgctttcac ctcgagcgcc agaccggcgg cctgacgcgc gacatcgagc gcggcacgcg
     1081 cgcgctgcag acgctggtgt cgtactcgct gtacacgatc atcccgacgc tggtggagct
     1141 cacgttcgtc ctggtgctgc tgggccaccg cttcgacatg ggctacgtgt ggatcacgct
     1201 ggccgcgctg ctggtctacg gcacgtacac gatctcgatc accgagtggc gcacgaagta
     1261 ccgcaaggcc gtcaacgagc tcgactccaa ggccaacacc aaggcgatcg acgcgctgat
     1321 caacttcgag acggtcaagt acttcaacaa cgagcagttc gagtcggcgc gctacgagac
     1381 cggcctggag gcctaccgcg cggcgcagat gaagtcgcag gccacgctgt cgatgctcaa
     1441 caccggacag cagctgatca tcgccggcag cctcgtggcg ctgctgtggc gcgccaccgg
     1501 cggcgtcatc gcgcactcga tgacgctggg cgacttcgtg atggtcaacg cgctgctgat
     1561 ccagctgtac gtgccgctga acttcctggg cgtgctgtac cgcgaggtca agcagggcct
     1621 gaccgacctg gagcgcatgt tcgcgctgct ggagaaggag cgcgagatcg ccgatgcgcc
     1681 ggacgccgtg ccgctgcggg tcgatggcgg cgccgtgcgc ttcgagcacg tgagcttcgc
     1741 ctacgagccg gcgcggccca tcctgcacga cgtcagcttc gagatccccg ccggcaagac
     1801 ggtggccgtg gtcggcccca gcggcgcggg caagagcacg ctggcgcggc tgctctatcg
     1861 cttctacgac gtcaccggcg gcgcgatccg catcgacggg caggacctgc gccaggtcac
     1921 gcagcacagc ctgcgcaagg cgatcggcat cgtgccgcag gacaccgtgc tgttcaacga
     1981 caccgtcgcc tacaacatcg cctacggccg gccgggggcg tcgcaggcgg aggtcgaggg
     2041 cgccgcgcgc gccgcgcaca tccacgactt catcgccgcc acgcccaagg gctacgagac
     2101 gatggtgggc gagcgcggcc tgaagctgtc cggtggcgag aagcagcgcg tcgcgatcgc
     2161 gcgcaccctg ctcaagaacc cgccgctgat gatcttcgac gaggccacct ccgcgctcga
     2221 ctccgccaac gagcgcgcga tccaggccga gctcgcgcag gccgcgcaag gacgcacgac
     2281 gctggtcatc gcgcaccggc tgtccacggt ggtcaacgcc gcgcagatcc tggtgatggc
     2341 cgagggccgc atcgtcgagc gcggcacgca ccccgaactg ctggcgatga atggccgcta
     2401 cgcgcacatg tggcggctgc agcagacgca cggcgacacg gccgcggcga ccgacgcgcc
     2461 cgggtcgccg acgctgggcg actcggcctt cgcggccgct gcgccggcgc gctg
//
LOCUS       DATCRO010000264         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_273010, whole genome shotgun sequence.
ACCESSION   DATCRO010000264 DATCRO010000000
VERSION     DATCRO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273010"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VIN75_07245"
     CDS             <1..1828
                     /locus_tag="VIN75_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY8973995.1"
                     /translation="KYLVDARTGALLRVDTGNQSLAPANPPALTASDVPAIGSGNSQY
                     SGVVPLSTTRRADGTFMLVDQTRGSKWNPWLHDSLWDMNGDPILDPNGNPVSVIGLQT
                     MAETHEGDVNDYTFAVAYHWFDENPTNAWGDGNQFVMYPYGMETSANGQTAAVDAHWG
                     MAVTWDFYRNVFGRDGVDNQGTSLVSEVHILGPFGYYYDNAFWDSFTMGMFYSDGTRN
                     VGVDPYTGGPTTPDPNGFDSLTAIDIIGHEMTHGVTAFSSGLVYDGESGGINESTSDF
                     MGSMVEAYSTRPAGADATIPETGTDWLMGAKVGNAPLRSMINPGMDGYSPNSWYSGIE
                     YLDVHYCSGPMNRFFYFLSQGSSPDPASPAYSRYLPGGMAGIGNDKAARIWYTAMTEW
                     LTPVDKYAQARVAAVNAAIELYGDGSSELQAVRNAFAAINVGSVSDTPGISITFSIQQ
                     PDGSLFNPGGNSFLARMPIVAMNSAVHLSADVRGTDDTRVTWRTGGMPGDLANPGFRV
                     DGGKVDADGNWTPDNLWGFHALTVTSDADPLEFAEGAAWVINGDADADNEFDAIDLGA
                     VALSWGLDQYVNASHSIVLDAWVDSFDVEAISQAFKNAYGGA"
     gene            1847..2536
                     /locus_tag="VIN75_07250"
     CDS             1847..2536
                     /locus_tag="VIN75_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973996.1"
                     /translation="MNTKLVSTLALFAALSGCGGGDSTPPAPPPAPAPAPMAAPTSLA
                     YTDPASSGWRLVKDASSTSTRLVLDLVGPAGTSTRGVGFNLKRGMGLAFGKFDNGGYA
                     HDTGVFQLKGTNPNFESYAGTDADPVLFASAPLKSGDVLSTGIFQKDRTNGAKDSAAP
                     LVQVVVTLADFTKVDPAAVAAATDPYGLHVIKARIVPADIGGFTLTTEVLKKARMVDI
                     TVDTGTITATP"
     gene            2557..>3404
                     /locus_tag="VIN75_07255"
     CDS             2557..>3404
                     /locus_tag="VIN75_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973997.1"
                     /translation="MNLHASQSEIDVLEGVLRRSPPGEGVVATKVALAWHLRQRDSGR
                     TLQLVQDVMPIVRASRASTHGDAIASCHARAALAAAEASALYGETVEAERCLGDARAH
                     LDPAWDPQADGDAWLVESLIARLRGDQDRVARGIGLAALAARGPGLAGGRLGAGRGAF
                     ERDPGLGHLQPLAILQPREAAARLAQRGLQSLGLSAQAGDQRLDQPGVALRLRIPRGI
                     EMGARVAQTALGLHRLAVQRRGFGRRERGARVAAGDGVAVRARARGAHDRHDVLHQLQ
                     RAAAVAL"
BASE COUNT          533 a         1282 c         1131 g          458 t
ORIGIN      
        1 gaagtacctc gtcgacgccc gcacaggcgc gctgctgcgc gtggacacgg gcaaccagtc
       61 cctggcgccg gccaacccgc cggcactgac cgcctcggac gtgcccgcga tcggcagcgg
      121 caacagccag tacagcggcg tcgtgccgct gtcgaccacg cggcgcgccg acggcacctt
      181 catgctggtc gaccagacgc gcggctcgaa gtggaatccg tggttgcacg actcgctgtg
      241 ggacatgaac ggcgacccga tcctcgaccc gaacggcaac ccggtgtcgg tgatcggcct
      301 gcagacgatg gccgagacgc acgaaggcga cgtcaacgac tacaccttcg ccgtcgcgta
      361 ccactggttc gacgagaacc cgaccaacgc ctggggcgac ggcaaccagt tcgtcatgta
      421 cccgtacggc atggagacga gcgccaacgg ccagaccgcc gcggtcgacg cgcactgggg
      481 catggccgtc acctgggact tctaccgcaa cgtgttcggc cgcgacggcg tcgacaacca
      541 gggcacgtcg ctggtctcgg aagtgcacat cctgggaccg ttcgggtact actacgacaa
      601 cgccttctgg gactcgttca ccatgggcat gttctattcg gacggcacgc gcaacgtcgg
      661 cgtcgatccg tacacgggcg gcccgacgac ccccgacccg aacggcttcg actcgctgac
      721 ggccatcgac atcatcggcc acgagatgac gcacggcgtc acggccttct cgtccggcct
      781 ggtctacgac ggcgagtcgg gcggcatcaa cgagtccacc tccgacttca tgggctcgat
      841 ggtcgaggcg tactcgaccc gcccggcggg cgccgacgcg accatccccg agaccggcac
      901 cgactggctc atgggcgcca aggtgggcaa cgccccgctg cgctcgatga tcaacccggg
      961 catggacggc tacagcccga actcctggta ctcgggcatc gagtacctgg acgtgcacta
     1021 ctgcagcggt ccgatgaacc gcttcttcta cttcctgtcg cagggctcgt cgcctgaccc
     1081 ggcgtcgccc gcatacagcc gctacctgcc cggcggcatg gccggcatcg gcaacgacaa
     1141 ggccgcgcgc atctggtaca cggcgatgac cgagtggctc acgccggtgg acaagtacgc
     1201 ccaggcgcgc gtggccgcgg tcaacgccgc catcgagctg tacggcgacg gctccagcga
     1261 gctgcaggcg gtccgcaacg cgttcgccgc gatcaacgtg ggcagcgtct ccgacacgcc
     1321 gggcatctcg atcacgttct cgatccagca gcccgacggc agcctgttca atccgggcgg
     1381 caacagcttc ctcgcgcgca tgcccatcgt cgcgatgaac tcggcggtgc acctgagcgc
     1441 cgacgtccgg ggcacggacg acacccgcgt cacctggcgc accggcggca tgcccggcga
     1501 cctcgccaac ccgggcttcc gcgtcgacgg cggcaaggtc gatgccgacg gcaactggac
     1561 gcccgacaac ctctggggct tccacgcgct gacggtcacg agcgacgccg acccgctgga
     1621 attcgccgag ggcgcggcct gggtcatcaa cggcgacgcc gacgcggaca acgagttcga
     1681 cgcgatcgac ctgggcgccg tcgcgctgtc ctggggcctc gaccagtacg tcaacgccag
     1741 ccacagcatc gtcctcgacg cctgggtcga cagcttcgac gtcgaagcca tttcgcaagc
     1801 cttcaagaac gcctacggcg gcgcctgaca cggaaaacct cgcatcatga acaccaagct
     1861 cgtctcgacc ctggccctct tcgccgcgct ctctggttgc ggcggcggcg actcgacgcc
     1921 gccggcgcct ccacccgcgc cggcgcccgc gccgatggcc gcgcccacca gcctggccta
     1981 caccgacccg gcgagctcgg gctggcgcct ggtcaaggac gcgtcgtcga cgtcgacgcg
     2041 cctcgtgctc gacctcgtcg gccccgccgg cacgtccacg cgcggcgtcg gcttcaacct
     2101 caagcgcggc atgggcctgg cgttcggcaa gttcgacaac ggcggctacg cgcacgacac
     2161 gggcgtgttc cagctcaagg gcaccaatcc gaacttcgag tcctacgccg gcaccgacgc
     2221 cgatccggtg ctgttcgcgt cggcgccgct gaagtcgggc gacgtgctgt ccaccggcat
     2281 cttccagaag gaccgcacca acggcgccaa ggactcggcc gctccgctgg tgcaggtcgt
     2341 ggtgacgctg gccgacttca cgaaggtcga cccggccgcc gtcgccgccg cgaccgaccc
     2401 ctacggcctg cacgtgatca aggcccgcat cgtgccggcc gacatcggcg gcttcacgct
     2461 caccaccgag gtgctcaaga aggccaggat ggtcgacatc acggtggaca ccggcaccat
     2521 caccgcgacg ccctgaccga ccgctgcaga ccggacatga acctgcacgc gtcgcaatcg
     2581 gagatcgacg tcctcgaagg cgtcctgcgg cgatcgccgc cgggcgaagg cgtggtcgcg
     2641 accaaggtgg cgctcgcctg gcacctgcgc cagcgcgaca gcggccgcac gctgcagctg
     2701 gtgcaggacg tcatgccgat cgtgcgcgcc tcgcgcgcga gcacgcacgg cgacgccatc
     2761 gccagctgcc acgcgcgcgc cgcgctcgcg gcggccgagg cctcggcgct gtatggcgag
     2821 acggtggagg ccgagcgctg cctcggcgat gcgcgcgcgc acctcgatcc cgcgtgggat
     2881 ccgcaggccg acggcgacgc gtggctcgtc gagtcgctga tcgcccgcct gcgcggcgac
     2941 caggatcgcg tcgcgcgcgg catcggtctc gcggcactcg ctgcccgcgg gccaggcctg
     3001 gccggagggc gcctcggggc aggccgcggt gcgttcgaac gcgatccagg cctgggccac
     3061 ctccagccgc tcgcgatcct ccagccgcgc gaagcagcgg ctcgcctggc gcagcgcggc
     3121 ctgcagtcgc tcggcctgtc cgcgcaggcg ggcgaccagc gactcgacca gccaggcgtc
     3181 gccctccgcc tgcggatccc acgcgggatc gagatgggcg cgcgcgtcgc gcagacagcg
     3241 ctcggcctcc accgcctcgc cgtacagcgc cgaggcttcg gccgccgcga gcgcggcgcg
     3301 cgcgtggcag ctggcgatgg cgtcgccgtg cgtgctcgcg cgcgaggcgc gcacgatcgg
     3361 catgacgtcc tgcaccagct gcagcgtgcg gccgctgtcg cgct
//
LOCUS       DATCRO010000265         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_632830, whole genome shotgun sequence.
ACCESSION   DATCRO010000265 DATCRO010000000
VERSION     DATCRO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632830"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VIN75_07260"
     CDS             <1..212
                     /locus_tag="VIN75_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEY8973998.1"
                     /translation="PRPAALEPLPAGLRWLGLDVRRHVQQDADHAIVEFVARSKLGGK
                     AERLHETSRFVREGGRWFYVDGDIA"
     gene            complement(267..797)
                     /locus_tag="VIN75_07265"
     CDS             complement(267..797)
                     /locus_tag="VIN75_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8973999.1"
                     /translation="MPQTYNVGSRSRFVNFIAWMFILLGAFACEWALIQNATQSWRAL
                     AADPATLPGLTGILMRHAPWVFSAAVALSLATMVCAAGLLRRIEWARRVFIGLLMVAI
                     AADLAGLWLQQEFLHLLVDPALQRTALTQPAAELFGGVVTVARVLAGLLTLVASLALA
                     AIIRRLMSPSVRQEFA"
     gene            1138..2025
                     /gene="rarD"
                     /locus_tag="VIN75_07270"
     CDS             1138..2025
                     /gene="rarD"
                     /locus_tag="VIN75_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY8974000.1"
                     /translation="MPGLVQAALAYVLWGLFPIYFHALSRVDAFRIVLHRSIWCFVFV
                     WGLLVAMRRLRWVPDVVRQPRLIGLFTVSALLLTSNWLLYVWAVNHGHVVEASLGYFV
                     TPLVNVMLGTLVLKERPRRLQWIAVAVAACGVLWLTLTLGRPPWIALGLAASFGCYGL
                     LRKTAPLGALEGLALETAVLAPVALVALALLAPGGPTGLFAGMDGTTIAWLLCAGPIT
                     AIPLLLFAGGARRITLATLGTLQYVSPTIQFLLGVAWFGEPLQATRLVGFVLIWAALA
                     IYSADGFLWMRRLRAQAAA"
     gene            complement(2033..3262)
                     /locus_tag="VIN75_07275"
     CDS             complement(2033..3262)
                     /locus_tag="VIN75_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8974001.1"
                     /translation="MTRPPITGDQTYVKSINRNVVLRLLLDEPGQSRATLAERSGLTK
                     STVSLLAKELLDEGWLAEDDVAVTGSLGRRPTPLRIAGRGLALIGAELGTDSIRVVAT
                     SLHGEVLQSMAVPLRDRAPDVACHQLVELVTAESRRVTRTGARLLGIGVGLPGAVDTA
                     HGVIQFAPNLGWHDVEVGQRLARELVAGGLAQVPVYCHNEADLAAIGETAFGSRPIDD
                     PLVYVSCAVGVGAGIVLKGELFTGATGAAGEIGHTILHVDGLPCTCGRRGCVEAYVGL
                     KAVAHAAGQLKAGEVDRDGLATDLANPRASRARKAFAAAGDALGVLLQNAWTTFNPKA
                     IVLGGEAVTLGGPHFLDAAVDRLARFGRDAGVAPPAVRVARYGDLAVAVGGAAFALNA
                     LLRPVGHDSGALRVNPD"
BASE COUNT          491 a         1307 c         1301 g          495 t
ORIGIN      
        1 ccccccgccc cgccgccctc gagcccctgc ccgccgggct gcgctggctg ggcctggacg
       61 tgcgccggca cgtgcagcag gacgccgacc acgcgatcgt cgagttcgtc gcgcgcagca
      121 agctgggcgg caaggccgag cggctgcacg agacgagccg gttcgtgcgc gaaggcgggc
      181 gctggttcta cgtcgacggc gacatcgcct gaaacagcga cggccgcaag cgcggccgtc
      241 gatcggggcg aagcgcgcaa gcgcctttag gcgaattcct gccgcaccga cggcgacatc
      301 aggcgacgga tgatggccgc cagcgccagg ctggccacca gcgtcagcag gccggcgagc
      361 acacgggcga cggtcacgac gccgccgaac agttcggccg ccggctgcgt gagcgcggtg
      421 cgctgcagcg ccgggtcgac cagcaggtgc aggaactcct gctgcagcca caggccggcg
      481 aggtcggcgg cgatggccac catcagcagg ccgatgaaga cgcggcgcgc ccactcgatg
      541 cggcgcagca ggcccgcggc gcagaccatc gtggccagcg acagcgcgac ggccgccgag
      601 aacacccacg gcgcatggcg catcaggatg ccggtgaggc cgggcagcgt ggccggatcg
      661 gcggcgagcg cccgccacga ctgcgtcgcg ttctggatca gcgcccactc gcacgcgaac
      721 gcgcccagca ggatgaacat ccaggcgatg aagttcacga agcgcgaacg cgatccgacg
      781 ttgtacgttt gcggcatggc tgtctcccga acgcaccgac catcatgaag tgcgttgatg
      841 acaaccattt ttcagtgtgc aggtgctcgc caacaatccc gcagaagggg gaactcgtgc
      901 gaaagcaacg gctggatgcg aactcctgcg cacttcgaga cgaacgtttc ttgcgcgcgg
      961 cgtgacgtcc acgtgacgtc gccgaaaggg tctggcaccg ccccggatcg cgccggggcg
     1021 atggcagccg cgcgcgacgc gacgccaccc cacgcttcgt cctcgcccga agtccggtca
     1081 ttcgcgggcc tccgataatg gcgcgcgccg ccgcccgcag gcggccacca gccctccatg
     1141 cccggtctcg tccaagccgc cctcgcctac gtcctctggg gcctgttccc gatctacttc
     1201 catgcgctga gccgcgtcga cgcgttccgg atcgtcctgc accgctcgat ctggtgcttc
     1261 gtgttcgtgt ggggactgct ggtggcgatg cggcggctgc gctgggtgcc ggacgtcgtc
     1321 cgccagccgc ggctcatcgg gctgttcacc gtcagcgcgc tgctgctgac cagcaactgg
     1381 ctgctgtacg tctgggccgt caaccacggc cacgtggtcg aggcgagcct gggctacttc
     1441 gtcaccccgc tggtcaacgt gatgctgggc acgctggtgc tcaaggaacg gccgcggcgg
     1501 ctgcagtgga tcgccgtggc ggtcgccgcc tgcggcgtgc tgtggctgac gctcacgctg
     1561 ggccgcccgc cctggatcgc gctgggcctg gccgcctcgt tcggctgcta cggcctgctg
     1621 cgcaagacgg cgccgctcgg cgcgctggag ggcctggcgc tggagacggc ggtgctcgcg
     1681 ccggtggcgc tcgtcgcgct ggcgctgctc gcgcccggcg gcccgaccgg cctgttcgcc
     1741 ggcatggacg gcacgaccat cgcctggctg ctgtgcgccg gcccgatcac cgcgatcccg
     1801 ctgctgctgt tcgccggcgg cgcgcgccgc atcacgctgg ccacgctcgg aacgctgcag
     1861 tacgtgtcgc cgacgatcca gttcctgctg ggcgtcgcgt ggttcggcga accgctgcag
     1921 gccacgcgcc tcgtcggctt cgtgctgatc tgggcggcgc tggcgatcta cagcgcggac
     1981 gggttcctct ggatgcggcg gctgcgggcg caggcggcgg cctgacggcg cgtcagtccg
     2041 gattgacccg caaggccccg ctgtcgtgcc ccaccggccg cagcagcgcg ttcagcgcga
     2101 acgccgcgcc gccgaccgcc accgccaggt cgccgtagcg ggcgacgcgc accgccggcg
     2161 gcgcgacgcc ggcgtcgcgg ccgaagcggg ccaggcggtc gacggcggcg tccaggaagt
     2221 gcgggccgcc cagcgtcacc gcctcgccgc ccagcacgat ggccttcgga ttgaaggtcg
     2281 tccaggcgtt ctgcagcagc acgccgagcg cgtcgccggc cgcggcgaag gccttgcgcg
     2341 cgcgtgacgc gcgagggttc gccaggtcgg tggccagccc gtcgcggtcg acctcgccgg
     2401 ccttgagctg gccggccgcg tgcgccaccg ccttcaggcc gacataggcc tcgacgcagc
     2461 cgcggcgtcc gcaggtgcac ggcaggccgt cgacgtgcag gatggtgtgg ccgatctcgc
     2521 cggccgcgcc ggtcgcgccg gtgaacagct cgcccttgag gacgatgccg gcgcccacgc
     2581 ccaccgcgca gctgacgtag accagcgggt cgtcgatcgg acggctgccg aacgccgtct
     2641 cgccgatggc ggccaggtcg gcctcgttgt ggcagtagac gggcacctgc gcgaggccgc
     2701 cggccaccag ctcgcgcgcc aggcgctggc ccacctccac gtcgtgccag cccaggttgg
     2761 gcgcgaactg gatgacgccg tgcgccgtgt cgaccgcgcc gggcaggccg acgccgatgc
     2821 ccagcaggcg cgcgccggtg cgcgtcacgc gccgggactc ggccgtgacc agttccacga
     2881 gctggtggca ggcgacgtcg ggcgcgcggt cgcgcagcgg caccgccatc gactgcagca
     2941 cctcgccgtg cagcgaggtc gccacgacgc ggatcgagtc ggtgcccagc tcggcgccga
     3001 tcagcgccag gccgcggccg gcgatgcgca gcggcgtggg ccggcggccc agcgaacccg
     3061 tgacggccac gtcgtcctcg gccagccagc cctcgtccag cagttccttg gccagcaggc
     3121 tcacggtgga cttggtcagc ccgctgcgct cggccagcgt ggcgcgcgac tggcccggct
     3181 cgtccagcag caggcgcagc acgacgttgc ggttgatcga cttgacgtag gtctggtcgc
     3241 ccgtgatcgg gggacgggtc atcggcgggt tctccatggc gggtctcgtc gcattgtccg
     3301 gccccgcgag gtggtgcccg gcgcggccgg gctgggctgg gctggacggg gcggtgtccg
     3361 caggctaggc gcctccgccg gatgccgcat tggggaaaac aagtaattgg ttcgaccgtc
     3421 gaacgaatta atcttcgccc cgcgctctgc aacaaagggc gagacgacgc acgacaacaa
     3481 ccggagacaa gccatgaacc tcaagcacgc actcttgacc gccggcctcc tcgcgggcgc
     3541 cgccgcggcc cacgcccagg tggtggtcgg cgtgagctgg tccaacttcc agga
//
LOCUS       DATCRO010000266         6198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_92712, whole genome shotgun sequence.
ACCESSION   DATCRO010000266 DATCRO010000000
VERSION     DATCRO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92712"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /gene="rplP"
                     /locus_tag="VIN75_07280"
     CDS             <1..359
                     /gene="rplP"
                     /locus_tag="VIN75_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8974002.1"
                     /translation="ATRGAAVSFGEFGLKATERGRITARQIEAARRAISRHIKRGGRI
                     FIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGKVLYEINGVPEQLAREAFTLAA
                     AKLPLSCTFVTRQIGM"
     gene            374..574
                     /gene="rpmC"
                     /locus_tag="VIN75_07285"
     CDS             374..574
                     /gene="rpmC"
                     /locus_tag="VIN75_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8974003.1"
                     /translation="MKASELRTKDVPALEKEVSDLLRAHFGLRMQKGTQQLTNHSQLK
                     NTRRDIARAKTILAEKKKGAAK"
     gene            571..861
                     /gene="rpsQ"
                     /locus_tag="VIN75_07290"
     CDS             571..861
                     /gene="rpsQ"
                     /locus_tag="VIN75_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY8974004.1"
                     /translation="MTQAQTAATEKPQTNTRTLVGRVVSDKRAKTITVLVERRHTHEL
                     YGKIVGRSRKYHAHDEKGEYKMGDLVEIAETRPISKTKSWVVTKLIEKARLA"
     gene            995..1513
                     /locus_tag="VIN75_07295"
     CDS             995..1513
                     /locus_tag="VIN75_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8974005.1"
                     /translation="MTIQVGDKLPAGTLKEFIEVETEGCSLGPNNVDVAAATAGKTIA
                     LFALPGAFTPTCSAKHVPGYVARFDALRAAGVDEIWCLSVNDAFVMGAWARDQKTAGK
                     VRMLGDGSAAFTQAVGLPLDLTANGMGVRSQRYSMLVVDGVVKTLNIEAPGKFEVSDA
                     DTMLKQAQALKG"
     gene            complement(1601..3646)
                     /locus_tag="VIN75_07300"
     CDS             complement(1601..3646)
                     /locus_tag="VIN75_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8974006.1"
                     /translation="MKSSRQPVLGVILAAWATLSTSASAADPAAAPATVPADTFFRRA
                     NMDDVSMSPDGGAIALLVRNAAGRRQLAVIDTADLHRVRIVASFDEYDIAWATWASDK
                     SLVFGLGDESASAAETRGSGLYSIGRDGQNLRVLINLNRGNFETGSHIKVRSLDPAAY
                     SFGGTLHDGSGDIVVLHWTANLKLGTFGSQWISTAPLRMNLQTGVMSEMGQKWPDHVF
                     QWVIDGQGRALAGVQTMEGQTTVLAADGAEWKPLARFPSYVPSADSFDVGDVGADGRM
                     YVSKAAEAAGARALYRYDLAAGKVEGEPVVSARGFDIAGDLVEDYHAHQVLGVHYDAD
                     AEGTVWFDPAMKALQAKIDARLPGLVNRIDPADCGCAQRVLVTSHSDHQPPVYFLYDR
                     SDDSLIAFGSSRPAVNPRQMADTDFVRIKARDGLEIPVYVTKPHGKGPWPTVVLVHGG
                     PWVRGWDWRWDPESQFLASRGYLVVKPEFRGSAGYGAALQSAGDRQWGLRMQDDIADA
                     TTWAAKQGLADPGRTCIAGASYGGYATLMGLVRYPDLYRCGVAWAAVSDIDLMYDIYW
                     SDADAEQKAFEMPALIGDQVKDAAQLAATSPLRQAARITHPLLLAHGGIDRRVPIEHA
                     TKLRSALEAAHAPVTWVEYKDEAHGWKKPETRIAFYQQMLDFLDANIGAKAAVATAH"
     gene            complement(3807..4556)
                     /locus_tag="VIN75_07305"
     CDS             complement(3807..4556)
                     /locus_tag="VIN75_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974007.1"
                     /translation="MKRQLLAAIAGLTLGAAAHAAPSYAVLSLVGDKLDIVTYQPVTG
                     SQLDPNSHTTLPMPQDELDVTALRTINRAVKAAVPDAQVALLAASTAADFEGQEHIFD
                     GNRVTLPAEIDAAVHRESVTTLVLVTKHQGEAHLEAWNDKLGSGRIRGLGFYVDGNKW
                     IRNRDTNLTSQGYVAPFVYVDVSVVDVASGTVTRRTTIESGRVVDSTRSTEGGNAWQA
                     LSAAQKVALLTNLLTEQLQSAVPRLLAATQH"
     gene            5125..5493
                     /gene="rplN"
                     /locus_tag="VIN75_07310"
     CDS             5125..5493
                     /gene="rplN"
                     /locus_tag="VIN75_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8974008.1"
                     /translation="MIQMQSRLDVADNTGAKSVQCIKVIGGSKRRYAGIGDVIKVSIK
                     EAAPRGRVKKGEVYNAVVVRTAKGVRRQDGSLIKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRSERFMKIVSLAPEVL"
     gene            5520..5837
                     /gene="rplX"
                     /locus_tag="VIN75_07315"
     CDS             5520..5837
                     /gene="rplX"
                     /locus_tag="VIN75_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8974009.1"
                     /translation="MNKIRKGDQVIVTTGRDKGKRGTVAERVSEQLLLVEGINVAKKH
                     VKPNPLKGTTGGVIEKTMPIQQSNVALFNPATGKADRVGFKILADGKKVRVFKSSGEE
                     VKA"
     gene            5848..>6198
                     /locus_tag="VIN75_07320"
     CDS             5848..>6198
                     /locus_tag="VIN75_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8974010.1"
                     /translation="MARLQTIYREKIVPDLTAKFGYKSVMEVPRLTKITLNMGVSEAV
                     ADKKVMDHAVGDLTKIAGQKPVVTKSKKAIAGFKIRDGVPIGCMVTLRGVQMYEFLDR
                     FVTIALPRVRDFRGI"
BASE COUNT         1035 a         2111 c         2085 g          967 t
ORIGIN      
        1 tcgccacccg tggcgccgcc gtctcgttcg gcgagttcgg cctgaaggcc accgagcgcg
       61 gccgcatcac ggcccgccag atcgaagctg cccgtcgtgc catctcgcgc cacatcaagc
      121 gcggtggccg catcttcatc cgcatcttcc cggacaagcc gatttcgcag aagccggccg
      181 aagtccgcat gggtaacggc aagggcaatc cggagtacta cgtggctgag atccagccgg
      241 gcaaggtgct ctacgagatc aacggtgtgc cggagcagct ggctcgcgaa gcgttcacgc
      301 tcgcggctgc caagctgccg ctgagctgca ccttcgtcac ccgccagatc ggcatgtgac
      361 ccggagcaaa tcaatgaaag catctgaact gcggaccaag gacgtcccgg cgctcgaaaa
      421 ggaagtctcc gacctcctgc gcgcccactt cggcctgcgc atgcagaagg gcactcaaca
      481 gctgacgaac cactcgcagc tgaagaacac ccgtcgtgac atcgctcgtg ccaagaccat
      541 cctggccgag aagaagaagg gagccgcgaa gtgacgcaag ctcaaaccgc cgcgaccgag
      601 aagccgcaaa ccaacacgcg taccctcgtg ggccgcgtcg tcagcgacaa gcgcgccaag
      661 accatcacgg tcctggtcga gcgccgccac acgcacgagc tgtacggcaa gatcgtcggc
      721 cgctcgcgca agtaccacgc ccacgacgaa aagggcgagt acaagatggg cgacctggtc
      781 gagatcgctg aaacccgtcc catctcgaag accaagagct gggtcgtgac caagctgatc
      841 gagaaggccc gcctggcctg atcgacgcgc tgcgcgggcc gcaaagcccg ccgccgcaca
      901 acgcggccgg aacgctggaa tactggcgtc ccggccgttt tcactttcag cgccgcataa
      961 agcgctggac cgacattcaa ctcaggagcg agacatgacc atccaggttg gcgacaagct
     1021 gcccgcgggc accctcaagg aattcatcga agtcgagacc gaaggctgct cgctcggtcc
     1081 gaacaacgtc gacgtggccg ccgccactgc cggcaagacg atcgcgctgt tcgcgctgcc
     1141 cggcgcattc acgcccacct gctcggccaa gcacgtgccg ggctacgtcg cccggttcga
     1201 cgcgctgcgc gccgcgggcg tcgacgagat ctggtgcctg tcggtcaacg acgccttcgt
     1261 catgggcgcc tgggcgcgcg accagaagac ggccggcaag gtgcgcatgc tgggtgacgg
     1321 ctcggccgcg ttcacgcagg ccgtgggcct gccgctggac ctgaccgcca acggcatggg
     1381 cgtgcgctcg cagcgctact cgatgctcgt ggtcgacggc gtcgtcaaga cgctcaacat
     1441 cgaggcgccg ggcaagttcg aggtcagcga cgcggacacg atgctcaagc aggcgcaggc
     1501 gctcaagggc tgaggcccgc gttcccgcag gccggacggg gccgcccgcg cgtcacgcgc
     1561 gcgcggccct ttccgtctcc ggcgccacag gcgggcgtcc tcagtgcgcc gtggcgacgg
     1621 cggccttggc gccgatgttg gcgtccagga agtccagcat ctgctggtag aacgcgatgc
     1681 gggtctcggg cttcttccag ccgtgcgcct cgtccttgta ctcgacccag gtcacggggg
     1741 cgtgcgcggc ctcgagcgcc gagcgcagct tggtggcgtg ctcgatcggg acacgccgat
     1801 cgatgccgcc atgggccagc agcagcgggt gcgtgatccg ggccgcctgg cgcagcgggg
     1861 aggtggccgc cagctgggcc gcgtccttga cctggtcgcc gatcagcgcg ggcatctcga
     1921 acgccttctg ctcggcgtcg gcgtcgctcc agtagatgtc gtacatcagg tcgatgtcgc
     1981 tcaccgccgc ccaggccacg ccgcatcggt acaggtcggg atagcgcacg aggcccatca
     2041 gcgtcgcgta gccgccgtag ctggcgcccg cgatgcaggt gcgccccgga tcggccagcc
     2101 cttgcttcgc ggcccatgtc gtcgcatcgg cgatgtcgtc ctgcatcctc aggccccatt
     2161 gtcggtcgcc ggcgctctgc agcgccgcgc cgtagcccgc gctgccgcgg aattccggct
     2221 tcacgacgag gtagccgcgc gacgcgagga actgcgactc gggatcccag cgccagtccc
     2281 agccgcgtac ccacgggccg ccgtgcacca gcaccacggt cggccatggg cccttgccgt
     2341 gcggcttggt cacgtagacc gggatctcga ggccgtcgcg cgccttgatg cgaacgaagt
     2401 ccgtgtcggc catctggcgc gggttcaccg cgggccggga cgatccgaat gcgatcagcg
     2461 aatcgtcgct gcgatcgtag aggaagtaga ccgggggctg gtgatcggag tgcgaggtga
     2521 caagcacgcg ctgagcgcag ccgcagtcgg cggggtcgat ccggttgacg aggcccggaa
     2581 gccgggcgtc gatcttcgcc tgcagcgcct tcatggccgg gtcgaaccac accgtgccct
     2641 ccgcgtcggc gtcgtagtgc acgcccagca cctggtgcgc gtggtagtcc tcgaccaggt
     2701 caccggcgat gtcgaagccg cgcgcgctca cgaccggttc gccctcgacc ttgcccgcgg
     2761 ccaggtcgta gcggtacagg gcgcgagccc cggcggcctc cgcggccttg ctcacgtaca
     2821 tccggccgtc ggcgccgacg tcgcccacat cgaagctgtc ggcgctcggc acgtagctcg
     2881 ggaacctggc cagcggcttc cattcggcgc cgtccgcggc aagcacggtc gtctggcctt
     2941 ccatcgtctg cactccggcg agcgcgcgac cctggccatc gatcacccac tggaagacgt
     3001 ggtcgggcca cttctggccc atctcgctca tgacgccggt ctgcaggttc attcgcagcg
     3061 gcgcggtcga gatccattgc gagccgaacg tgcccagctt cagattggcg gtccagtgca
     3121 gcacgacgat gtcgccgctg ccgtcgtgca acgtgcctcc gaagctgtag gcggccgggt
     3181 cgagcgagcg caccttgatg tggctgcccg tctcgaagtt gccccggttg aggttgatca
     3241 gcactcgcag gttctggccg tcgcgtccga tgctgtacag gcccgagccg cgcgtctcgg
     3301 cagccgaagc gctttcgtcg ccgagtccga acacgagcga cttgtcgctg gcccacgttg
     3361 cccaggcgat gtcgtactca tcgaagctcg cgacgatcct gacgcggtgc aggtcggcgg
     3421 tgtcgatcac cgccagctgg cgccggccgg ccgcgttgcg caccaggagg gcgatggcac
     3481 cgccgtccgg cgacatgctg acatcgtcca tgttcgcgcg ccggaagaac gtgtcggcag
     3541 gcacggtagc cggcgcggcg gccggatcgg cggccgaggc actcgtggac agggtggccc
     3601 atgcggccag gatgacgccc aggacgggct ggcgtgatga cttcacggaa agactccctc
     3661 gatctgcaag cggaagcggc gctccatggg cgccggcgga ttcgtgcgac agcaccgggc
     3721 tgtcgtgccg gccgattgtg gcttgagtcg agagacgaat cggcgcggcg gcgccggatt
     3781 cgcgttcagg ttttcccggg ccggcctcag tgctgcgtgg ccgccagcag ccgcggcacc
     3841 gcggactgca gctgctcggt cagcaggttg gtcagcaagg ccaccttctg cgcggcactg
     3901 agcgcctgcc aggcattccc gccctcggtg ctccgcgtgg agtcgacgac acggcccgac
     3961 tcgatcgtcg tgcgccgggt caccgtgccg ctggccacgt ccaccaccga cacgtccacg
     4021 tagacgaacg gcgcgacgta gccctgcgag gtgaggttgg tgtcccggtt gcggatccac
     4081 ttgttgccgt cgacgtagaa gcccagcccg cggatgcggc cgctgccgag cttgtcgttc
     4141 cacgcctcca ggtgggcctc gccctggtgc ttggtcacga gcaccagcgt ggtgacgctc
     4201 tcgcggtgga cggcggcgtc gatctccgcc ggcagcgtca cgcggttgcc gtcgaagatg
     4261 tgctcctggc cctcgaaatc ggcggcggtg gaggccgcca gcaacgccac ctgggcgtcg
     4321 ggcacggcag ccttgaccgc ccggttgatc gtgcgcagcg cggtgacgtc cagctcgtcc
     4381 tgcggcatgg gcaacgtcgt gtgcgagttc gggtccagct gcgagccggt gaccggctgg
     4441 taggtgacga tgtcgagctt gtcgccgacg agggacaaca cggcgtagct gggcgccgcg
     4501 tgggccgccg cgccgagcgt caggccggcg atcgccgcaa gcagttgtcg tttcatggtt
     4561 cgttctccct ggttgtctgt cgaaggaccg catcatcacc gggccggcct gagcggacgc
     4621 tgacttgtcg gaggcccgcg ctgccgccgt gcgctccgga cgtctgcggg cactgtaggc
     4681 gcgcgcccga gccgtgtcgc ccgtcccgcg acgcaggacg gccccgcgcc gaccaaacgc
     4741 ctgttccggg cgatgaacgg cgatccgccc ggcgccggtg ccgggctgcc gtcgagcccg
     4801 gcgcgccggg acgccggtca ggcgggccca tcgtccggcg ggcctgcacg acggcgacgt
     4861 cagggtgttt gacatgtcgt ccaccttcga tgcataatgg ccgacttcgc cgaagaggcg
     4921 tgagcaacgc atgccgcttc cagtcaacac tggaggcgtc tggagtgacc agaaggctgc
     4981 gctgcttgtc tccgaggaat ttcagcccaa ccggggtgtt tcggaagccg caggccctgc
     5041 ctgcagtgaa agagatgtca cgacgggccc aatactgacc gctctcgggt gttgccgtct
     5101 gcgggagcaa gttggggata aaccatgatt caaatgcagt cgcgactcga cgtcgcggac
     5161 aacaccggtg cgaagtccgt ccagtgcatc aaggtcattg gcggctcgaa gcgccgctat
     5221 gccggcattg gtgacgtgat caaggtcagc atcaaggaag ccgcgccgcg tggccgcgtg
     5281 aagaagggcg aggtctacaa cgccgtcgtc gttcgcaccg ccaagggcgt gcgtcgccag
     5341 gacggctcgc tgatcaagtt cgacggcaac gccgccgtcc tgctcaacgc caagctggag
     5401 cccatcggca cccgcatctt cggccccgtg acgcgcgaac tgcgctccga gcgcttcatg
     5461 aagatcgtgt cgctggcccc ggaagtgctc taagcgcgcc cgcagtacaa ggaaacgcca
     5521 tgaacaagat ccgcaagggc gaccaggtca tcgtgacgac cggtcgtgac aagggcaagc
     5581 gcggcaccgt cgccgagcgc gtcagcgaac agctgctgct cgtcgagggc atcaacgtcg
     5641 ccaagaagca cgtcaagccg aacccgctga agggcaccac cggtggtgtc atcgagaaga
     5701 cgatgcccat ccagcagtcc aacgtcgcgc tattcaaccc ggcgaccggc aaggccgatc
     5761 gcgtgggctt caagattctg gccgacggca agaaggttcg cgtcttcaag tccagcggcg
     5821 aagaagtcaa ggcataagag gtccgacatg gctcgtctcc aaaccattta ccgcgaaaag
     5881 atcgtcccgg acctcacggc caagttcggc tacaagtccg tgatggaagt gccgcgcctg
     5941 accaagatca ccctgaacat gggtgtgtcc gaggccgtcg ccgacaagaa ggtcatggac
     6001 cacgccgtgg gcgacctgac caagatcgcc ggccagaagc ccgtggtcac caagtcgaag
     6061 aaggccatcg ccggcttcaa gatccgtgac ggcgtgccca tcggctgcat ggtcacgctg
     6121 cgtggcgtcc agatgtacga attcctggac cgcttcgtga cgatcgccct gccgcgcgtg
     6181 cgcgacttcc gtggtatc
//
LOCUS       DATCRO010000267         8946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_81692, whole genome shotgun sequence.
ACCESSION   DATCRO010000267 DATCRO010000000
VERSION     DATCRO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81692"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VIN75_07325"
     CDS             <1..499
                     /locus_tag="VIN75_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974011.1"
                     /translation="GNATGSISHGAPSTGTPSTAAPSTTTPPATPAAHSIAGNVAGTG
                     SATASHAHDTGSSTTPSTSGRSLTGNVAGNGAATASRTHDTGSSTTPSSSGRSVSGNV
                     AGNGSGSATATRSTGSGSRSVAANGSGNGALQGSASRHDGVNVGANGSASGSMQAGHP
                     TQSGN"
     gene            complement(752..1864)
                     /locus_tag="VIN75_07330"
     CDS             complement(752..1864)
                     /locus_tag="VIN75_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8974012.1"
                     /translation="MFPFLSRFKRSLLVALVLIASSAAADERRLVIIDQDMFGPGGSN
                     MNSVLMLLQAPDVDVLGIVVSSGDGWRDEEIAQTLRMLELVGRPEVPVYAGATFPLLN
                     SAARTKAWERRYGPLVWKGAWTESFGGKPRAQYHSDPFLVPPLVAGAPSLKVQAESGV
                     SFMIRTVRQHPGRVTLWMGGPLTDLALAARQDPGFASAARELVFMGGSFNPVAADNGF
                     AEEYVHSPRREFNMLWDAESASAVLHEAWPRIVQVPVDPTTKTFFRTELYAEIGHAGS
                     PVAAYVARYGEGFPMWDELAAAVWLDPSLVRRSQKMLEDVAAGDGADYGSTLSWPIGS
                     GPGLGERVVEVVQDVDVPRFERLTVKLLSARRAPGH"
     gene            complement(2125..2622)
                     /locus_tag="VIN75_07335"
     CDS             complement(2125..2622)
                     /locus_tag="VIN75_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974013.1"
                     /translation="MTIIPQPLHEFAGRQTVALVLLAATGIADAQTWRCETADGRVEY
                     RDRECAASAAKASAGPAPAPAAQASLPQGRQARREARGAPTRVPVNKHPVSLEFTDVR
                     IDEVMSILARFDGRRLVVDPSVAGHRIACHYRNMPWDAAVADIAARGDVDISYDDRQI
                     MVKKR"
     gene            complement(2689..3462)
                     /locus_tag="VIN75_07340"
     CDS             complement(2689..3462)
                     /locus_tag="VIN75_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8974014.1"
                     /translation="MKLHSWDISPYAARVRMQIYAKGLTHIATEQAPFLLTQKSYEEC
                     PLSRIPSLETDDGHAIPESAVIAEYLEELYPEPSLLGSSPSENAHIRTLARIGDLYLI
                     NTAFTLASLTRTLADKPDPAGIESVAAQLGGQLASSIRTLDRMIGSDGFACLGRLTLA
                     DCALVPALSYVQFMLMNRGLPDPIAANANVAAYQAAIQEQEPAARVLAEFRRGREERR
                     EQIASGELEKIRAANQAAREAAERATSARPPSVHGVGQP"
     gene            3451..4563
                     /locus_tag="VIN75_07345"
     CDS             3451..4563
                     /locus_tag="VIN75_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974015.1"
                     /translation="MEFHGHVASPSLHGASLHDEIEYARILVSATRFFKFIIFTTIML
                     AKHDSASSPENPQGIALLEDLRAFVEFARAGSIAGAADRLFRTPSAVTRQVQRLEAAL
                     GAELLDRSVKPPRLNALGSRVLEQAQDLLQRTEALKSLTSFGAEPEGLLRIGISYTLA
                     EEMLVEPIRALNEKYPKVRVQLSSEQLIQRLLAGELDVAALPLPQGRTAPAPLLTNII
                     ATDRMEIVQGGAGKLDADWKALGRAPWVLNPPGCFLRASLIDRMERAGFTPRIAAEVN
                     NIQMQLAFVQAGYGVGLLPSRFIARNRSIGHLKILRPPSFDLHRSVGIVRAGQLGALG
                     KVVDLLEDGLRTLLEAPEVTRPASRRTGARAKHSRR"
     gene            complement(4628..5149)
                     /locus_tag="VIN75_07350"
     CDS             complement(4628..5149)
                     /locus_tag="VIN75_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8974016.1"
                     /translation="MTGPTLRPRVPAGPVLAASLALALALSAARTIHEAPAAMRDHGP
                     HALMAPPAAPDADGAPARPATIVRPLSCTPLPDVPGSSVTTALVEFPPGARTGPHRHP
                     GSVTAFVISGTVRSQMAGGPAQDYTKGQTWFEAPQALHLFAENPSPTAPAILLATFIT
                     DDGCTKLVIPEPH"
     gene            complement(5146..5889)
                     /locus_tag="VIN75_07355"
     CDS             complement(5146..5889)
                     /locus_tag="VIN75_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8974017.1"
                     /translation="MKIFVAGASGAIGRRLVPLLVQAGHEVHGGTRGERGAAVLRALG
                     ATPCVADVFDRDALHATLRRSRPDVVIHQLTDLPPGLDPARMGDAIARNARVRSEGTA
                     NLVAAALASGCGRMVAQSIAWAYAPGDGARRETDPLDLAAVGARATTVQGVAALEAAV
                     LGTPALAGTVLRYGHLYGPGTGTGQTADPLRVHVDAAASAALLALRPDALGAFNICEP
                     NEQVSPARAVDLLGWSAAFRLPEAAGSAR"
     gene            5980..7440
                     /locus_tag="VIN75_07360"
     CDS             5980..7440
                     /locus_tag="VIN75_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8974018.1"
                     /translation="MIDIPGLLDRPLTRAGALPLHRQIYERVRRAILQGQLAPGQRLA
                     SSRALASQLGLARGTVDAAYAQLAVEGYLEALGQKGTRVSLEVRRAAARATSPREPKS
                     APQPDGLPAPLLPFQMGMPAFDAFPRKLWASMGARRVRAVGVDDLAYPDPRGSPRLRR
                     AIAAYLLVSRGVDCDPDQVFVTAGHRASLDLVLRLLVRPGDRVCIEDPYYPAAFELVR
                     GAGARVACVPVDDHGLRVDRLVARHADARLALVTPAHQAPMGVSLTLARRMALLAWAG
                     ARRAWIVEDDYDSEFRYVGAPLPALHSADVAGCVLYAGSFSKVLHPGLGLAYLVVPPS
                     QVRAFTQHVVRSSNGCSPLQQELVADFMELGHFPRHIRKMRTLYARRRQWLVEALHAA
                     FGSQVRIDLQAGGMHLLARFPRHGADAELAERARACGLAAQALSRRSSGSHAGQGLLL
                     GFTNIRSLEEAQAQAAQLRSALGIVAGAGAGRVRRP"
     gene            complement(7488..7901)
                     /gene="arr"
                     /locus_tag="VIN75_07365"
     CDS             complement(7488..7901)
                     /gene="arr"
                     /locus_tag="VIN75_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEY8974019.1"
                     /translation="MSTAASMFAPSFFHGTRADLQPGDLIVVGHPSNFTGGDSLSWVY
                     FSGTLDAAVWGAELAIGDGPGRIYVVEPTGEVEDDPNLTDKKYPGNPTRSFRSREPLR
                     IIGEVTRWEGHPPERLRQMQAHVARARAQGAGIID"
     gene            complement(8003..>8946)
                     /locus_tag="VIN75_07370"
     CDS             complement(8003..>8946)
                     /locus_tag="VIN75_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8974020.1"
                     /translation="ARDWLAQGHAVWLVTVLETWGSAPRPPGALLALRDDGPVVGSVS
                     GGCVEDDLIDRVRHGERVAAPALVSYGVTREEAARFGLPCGGTLRLVQEPLRDAAWIE
                     EILARCARHELVARTLELATGAVAVEPATRGERFSFDGQRMRTLFGPRWRLVIIGAGQ
                     LSRVVAPMALALDFEVLCCDPREEYHVTWDVPGTEFSREMPDDLVQRLQLDAHSALVA
                     LTHDPKLDDLALMEALKSAAFYVGALGSRANTARRKERLAMFDVSPEEIGRLHGPVGL
                     DLGGRTPAEIAVSILAEIVAVKNEVAIVQKKPALSAG"
BASE COUNT         1294 a         3170 c         3158 g         1324 t
ORIGIN      
        1 gggcaatgcc accgggtcga tctcgcacgg cgcccccagc accggcacgc cgtcgaccgc
       61 cgcaccgtcg acgacgaccc cgcccgcgac gcctgccgcg cactcgatcg cgggcaatgt
      121 cgccggcacc ggcagcgcga cggcctcgca cgcgcacgac accgggtcgt cgacgacgcc
      181 gtcgaccagc ggccgctcgc tgacgggcaa cgtcgctggc aacggcgcgg cgaccgcctc
      241 gcgcacgcac gacacgggtt cgtcgaccac cccgtcgtcc agcggccgct cggtctccgg
      301 caacgtcgcc ggcaacggca gcggctcggc gacggccacg cgctcgaccg gttcgggcag
      361 ccgctccgtc gcggccaacg gctcgggcaa cggcgcgctg cagggttccg cgtcgcgcca
      421 cgacggcgtg aacgtcggcg cgaacggctc ggcgagcggc tcgatgcagg ccggccaccc
      481 gacgcagtcc ggcaactgat cgcgaggggc acgcagcgat gcggcccgcc tcggcgcggc
      541 cagccttcca tgggctggcc gcctattttc gactcgtcgt ccaggccatg acccgcctcg
      601 ccacccaccc cgaagcccgc gccaggctgg ccgtgactgc tgacgccctc cgacgggatc
      661 gtgccggcga gcgaacgggc ttccctcgcg ctcgattgac gaccgaaccg cacgccggaa
      721 tggtcatggc aacgctcgcc cacgacgcct gtcaatgtcc gggcgctcgt ctcgcgctca
      781 gcagcttgac ggtcaagcgt tcgaagcgcg gcacgtccac gtcctggacg acctcgacca
      841 cgcgctcgcc caggcccggg ccgctgccga tcggccagct gagcgtgctc ccatagtccg
      901 caccatcgcc ggccgccacg tcttccagca tcttctggct gcgcctgaca agcgatggat
      961 ccagccacac ggccgccgcc agctcgtccc acatcggaaa accctctcca tagcgcgcga
     1021 cataagcggc gaccggcgag ccggcatggc cgatctccgc gtacagctcc gtgcggaaga
     1081 acgtcttcgt cgtcgggtcg accgggacct gcacgatgcg cggccacgct tcgtgcagca
     1141 cggccgatgc cgattcggcg tcccacagca tgttgaactc gcgccgcggc gaatggacat
     1201 attcctcggc gaacccgttg tccgctgcaa cggggttgaa gctcccgccc atgaagacga
     1261 gctctcgcgc cgccgaggcg aagcctggat cttgtcgcgc ggccagcgca aggtcggtca
     1321 acgggccgcc catccagagc gtcacacggc ccggatgctg ccgcacggtg cgaatcatga
     1381 aactgacgcc gctctcggcc tgcaccttca gagagggggc gcccgcaacc agcggcggca
     1441 ccaggaacgg gtcggaatgg tactgggcgc gcggctttcc cccgaacgac tccgtccatg
     1501 cgcccttcca caccagaggt ccatagcgcc gctcccaggc cttcgttcgc gcggcgctat
     1561 tgagcaacgg gaacgtcgcg cccgcgtaga cgggcacctc tggacgcccg accagctcca
     1621 gcatgcgcaa ggtctgagcg atctcttcgt cacgccagcc gtcgccgctg ctgacgacga
     1681 ttcccagcac atcgacgtcc ggcgcctgaa gcagcatcag caccgagttc atgttgctgc
     1741 cgccgggccc gaacatgtcc tggtcgatga tgaccaggcg acgttcgtca gcggcggcgg
     1801 aagaagcgat cagcaccagc gcgaccagca gcgatcgctt gaaacgtgag aggaacggga
     1861 acatggccgg cctttgcatg aagagatgcc agcggtgcaa gcaaggccca taccgtggag
     1921 tccaggcacg tcgcccccag ttcgccacgc cgttcaagtc cccctgacgg cgcattgcgc
     1981 agcggcgaat cttgcgagag tcatgacggc tctggaccgg cctgcttcga atgttcggtc
     2041 cggctgcgag cggaggttcg tcgaactcgg catgcggcct ggcctccacc ttgtcgatgc
     2101 ctgccaacag cggcgtgccc gaattcagcg cttcttcacc atgatctggc gatcgtcgta
     2161 gctgatgtcc acgtcgcccc gggcggcgat gtccgccacg gccgcgtccc aaggcatgtt
     2221 cctgtagtga caggcgatgc gatggccggc gacggatggg tcgaccacga gccgcctgcc
     2281 gtcgaacctg gccaggatgg acatcacctc gtcgatgcgg acgtcggtga actcgaggct
     2341 gaccgggtgc ttgttcacgg ggacgcgggt cggggctccg cgggcttcgc gccgtgcttg
     2401 ccggccctga ggcagcgatg cctgcgcggc aggggccggc gccgggccgg cgctcgcctt
     2461 cgcggcggat gccgcgcact cgcggtcacg gtactcgacg cgcccatccg ccgtctcgca
     2521 gcgccaggtt tgcgcgtcgg cgatgccggt cgcggcgaga agcacgaggg cgacagtctg
     2581 ccggccggcg aactcatgca ggggctgggg gatgatggtc atggccttgt ccttcacgga
     2641 tttgcttcgt gtacgcaact cttccgagcc aatgacgccc agcatagttc agggctgtcc
     2701 gacaccgtgc acggatggag gccgcgcgct cgtcgcccgc tccgccgctt cccttgcggc
     2761 ctggttcgcc gccctgatct tctcgagctc gccgctggcg atctgttcgc ggcgctcctc
     2821 ccgccctcgg cggaactcgg cgaggacgcg cgccgcaggc tcctgttcct ggatggctgc
     2881 ctggtaggcg gccacgttgg cgttcgctgc gatcgggtcg ggaagtcccc ggttcatcag
     2941 catgaactgc acataggaga gcgccggcac gagcgcgcaa tcggccaggg tcaggcggcc
     3001 gaggcaggcg aacccgtcgc tgccgatcat ccggtccaag gtcctgatgc tgctcgccag
     3061 ttgcccgccg agctgcgcgg cgacgctctc gatcccggcg ggatcgggct tgtcagcgag
     3121 ggtccgggtc agagacgcga gcgtgaacgc ggtgttgatc aggtacaggt cgccgatacg
     3181 cgccagcgtg cggatgtgcg cgttctcgct cggcgacgag cccagcagcg agggctcagg
     3241 gtagagctcc tcgagatact cggcgatcac cgccgactcc ggtatggcgt gtccgtcgtc
     3301 ggtctccagc gacgggatgc ggctgagcgg gcattcttcg taggacttct gcgtcaggag
     3361 aaatggcgcc tgctcggtgg cgatatgggt gagacccttg gcgtagatct gcatccgcac
     3421 ccgcgccgcg taagggctga tgtcccagga atggagtttc atggtcacgt cgcttctccg
     3481 agtcttcatg gggcgtccct gcacgacgag atcgaatacg cacgcattct ggtgagcgcg
     3541 acaagattct tcaagttcat tattttcacg actatcatgc tggcaaagca cgattccgct
     3601 tccagcccag agaacccgca ggggatcgcc ttgctcgaag atttgcgcgc cttcgtggag
     3661 ttcgcccggg ccggctcgat cgccggtgca gccgatcgcc tgtttcgcac tccgtctgcg
     3721 gtcacccggc aagtgcaaag gctcgaggcc gcgttgggcg ccgaattgct cgaccgctcc
     3781 gtgaagccgc cgcgcctgaa cgcgctgggc tccagggtgc tcgaacaagc gcaggacctg
     3841 ctgcagcgga ccgaggcgct caaatcgctc acgtcgttcg gcgctgaacc cgagggcctg
     3901 ctgcgcatcg ggatctccta caccctcgcc gaggaaatgc ttgtcgaacc gatccgggcg
     3961 ctgaacgaga aatacccgaa ggtgcgggtg cagctttcga gcgagcaatt gatccagcga
     4021 ctgctcgcgg gtgaactcga cgtcgccgcc ttgcccttgc cccaaggcag gacggcgccg
     4081 gcacccctgc tcacgaacat catcgccacc gatcgcatgg agatcgtcca gggcggcgca
     4141 ggcaagcttg atgccgactg gaaagcgctg ggccgggcgc cttgggtgct caatcccccc
     4201 ggttgcttcc ttcgggccag cttgatcgat cgaatggagc gtgccggctt cacccccagg
     4261 atcgcggccg aagtcaacaa catccagatg cagctggcgt tcgtgcaggc cggttatggc
     4321 gtcgggctgc tgccgtcccg attcatcgcc aggaatcgtt cgatcggtca cttgaagatc
     4381 ttgcgccccc catcgttcga tctgcaccga tccgtcggga tcgtccgggc gggccagttg
     4441 ggcgccttgg gaaaggtcgt cgacttgctg gaggacgggc ttcgcacgtt gctcgaggcg
     4501 cccgaggtca cccggccagc aagcaggagg accggcgcgc gggcgaagca ttcgcggcgc
     4561 tgatctccat gacagttcct ggcccggctt gtcgcctgca cgcggcaggc cggggccgcg
     4621 catgaactca gtgcggctcg ggaatcacga gcttggtgca accgtcgtcg gtgatgaacg
     4681 tcgccagcag gatcgcgggc gccgtcgggc tcgggttctc ggcgaacagg tgcagcgcct
     4741 gcggagcctc gaaccatgtc tggcccttcg tgtagtcctg cgccgggccg ccggccatct
     4801 gcgaacgcac ggtgccggag atgacgaatg cggtgacgga accgggatgg cggtgcgggc
     4861 cggtgcgcgc gcccggcggg aactccacca gggccgtcgt cacgctgctg cccggcacgt
     4921 cgggcagcgg cgtgcaggac aaggggcgca cgatcgtcgc gggtcgcgcc ggggcgccgt
     4981 cggcatccgg cgcggccggc ggcgccatca gcgcatgagg gccgtggtcg cgcatcgcgg
     5041 cgggcgcctc gtggatcgtc cgcgccgccg acagcgcgag ggcgagcgcg agcgatgctg
     5101 cgagcaccgg ccctgccggc acgcgaggtc gaagcgtcgg gccggtcatc gtgcgctccc
     5161 cgcggcctcg ggcaggcgga acgcggccga ccagcccagg aggtcgacgg cgcgtgcggg
     5221 cgacacctgc tcgttcggct cgcagatgtt gaacgcgccg agcgcgtccg gccgcagggc
     5281 cagcagcgcg gccgaggcgg cggcgtccac gtgaacgcgc agcggatcgg ccgtctggcc
     5341 ggtgcccgtg cccggcccgt agaggtggcc atagcggagg acggtgccgg cgagcgcggg
     5401 cgtgcccagc acggccgcct cgagcgcggc gacgccttgc accgtggttg cgcgagcgcc
     5461 gacggcggcg aggtccagcg gatccgtttc gcggcgggcg ccgtcacccg gtgcataggc
     5521 ccaggcgatg ctctgggcca ccatgcggcc gcaaccggac gccagcgcgg cggcgaccag
     5581 gttggcggtg ccttcggagc ggacgcgcgc attgcgggca atggcgtcgc ccatccgggc
     5641 ggggtccagg ccgggcggca ggtcggtgag ctgatggatg acgacgtccg gccgcgaccg
     5701 ccgcagcgtc gcgtgcaggg catcgcggtc gaacacgtcg gccacgcagg gtgtcgctcc
     5761 gagcgcgcgc agcacggcgg ccccgcgctc gccgcgtgtc ccgccgtgca cctcgtggcc
     5821 cgcctggacg agcaggggaa ccaggcggcg gccgatggcg ccactggcgc cggcaacgaa
     5881 gattttcatg atgagctccg gaagtgaggc tgtcagggct cactgtagga ataggatggc
     5941 ctacctggaa gtgccaagaa ttcgaaaacg gactgggcca tgatcgacat ccccgggttg
     6001 ctcgaccgac cgctgacgcg cgccggcgcg ctgccgctgc accggcagat ctacgaacgc
     6061 gtgcggcggg cgatcctgca ggggcagctc gcgccgggcc agcgcctggc gtcctcccgc
     6121 gcgctggcga gccagctggg cctggcgcgc gggacggtcg acgcggcgta tgcgcagctg
     6181 gccgtcgaag gctacctcga ggccctcggc cagaagggca cgcgggtctc gctcgaggtg
     6241 cgccgcgccg ctgcccgcgc gacctcgccg cgcgaaccga agtcggcgcc gcagcccgat
     6301 ggcctgccgg ccccgctgct gccgttccag atgggcatgc ccgcgttcga tgcgtttccg
     6361 cgcaagctct gggcgagcat gggcgcgcgc cgcgtgcgcg ccgtcggcgt cgacgacctc
     6421 gcctatcccg acccacgcgg atcgccgcgc ctgagacgcg cgatcgcggc ctacctgctg
     6481 gtctcgcgcg gcgtcgactg cgacccggac caggtcttcg tcaccgcggg acaccgcgcc
     6541 agcctcgacc tggtgctgcg cctgctcgtg cggccgggcg atcgcgtctg catcgaggat
     6601 ccatactatc cggccgcgtt cgagctcgtg cgcggcgcgg gcgcgcgcgt ggcctgcgtg
     6661 ccggtggacg accacggcct gcgcgtcgac cggctcgtcg cgcgccacgc cgatgcgcgg
     6721 ctggcgctgg tgacgccggc ccaccaggcg ccgatgggcg tgtcgctgac gcttgcacgg
     6781 cggatggcgt tgctggcctg ggccggcgcc aggcgcgcct ggatcgtcga ggacgactac
     6841 gacagcgagt tccgctacgt cggcgcgccg ctgccggcgc tgcacagcgc cgacgtggcc
     6901 ggatgcgtcc tgtacgcggg cagcttcagc aaggtgctgc atcccgggct cggactggcc
     6961 tacctcgtcg tgccgccgtc ccaggtgcgc gccttcacgc agcacgtcgt gcgttcgagc
     7021 aacggctgct cgccgctgca gcaggagctg gtggccgact tcatggagct cggccacttc
     7081 ccgcgccaca tccgcaagat gcgcacgctc tatgcgcggc gccgccagtg gctcgtggag
     7141 gcgctgcatg cggccttcgg gtcgcaggtg cgcatcgacc tgcaggccgg tggcatgcac
     7201 ctgctcgcac gcttcccgcg gcacggcgcg gacgccgaac tcgccgagcg agcgcgggca
     7261 tgcggactcg cggcccaggc gctgtcgcgg cgttcgtcag gcagccacgc ggggcagggc
     7321 ctgctgctgg gcttcaccaa catccgctcc ctggaggagg cgcaggcgca ggcggcgcag
     7381 ctgcgcagcg cgctgggcat cgttgcgggc gcgggcgcgg gacgtgtccg gcggccatga
     7441 tgtccagccg ccgcgccaat gctgcggccc gccacatgcc cggacggtca atcgatgatg
     7501 ccggcgccct gcgcccgtgc gcgggcgaca tgcgcctgca tctgccgcag gcgttcgggc
     7561 gggtgccctt cccagcgcgt gacctcgccg atgatccgca gcggctcgcg cgagcggaac
     7621 gaccgtgtcg gattgccggg atacttcttg tccgtcaggt tcggatcgtc ctcgacctct
     7681 ccggtcggct ccacgacgta gatgcgcccg ggcccgtcgc cgatggccag ttcggcgccc
     7741 cagacggcgg cgtccagcgt gccggagaag tacacccacg acagcgaatc gcccccggtg
     7801 aagttcgatg gatgcccgac gacgatcagg tctccgggct gcaggtcggc acgcgtgccg
     7861 tggaagaacg acggagcgaa catgctggcg gcggtggaca tggatcggcg gctctcgttc
     7921 gtacgggact cggatcccgt gatcaggtac cggttgcggg ccggctagcg gcgaccggcc
     7981 gcggcatgcg gcgcgcctgg tctcaaccgg ccgacagcgc cggcttcttc tgcacgatcg
     8041 cgacctcgtt cttgaccgcg acgatctcgg ccaggatcga caccgcgatc tcggccggcg
     8101 tgcggccgcc taggtccagg ccgacggggc cgtgcaggcg gccgatctcc tcgggcgaga
     8161 cgtcgaacat cgcgaggcgc tccttgcggc gcgccgtgtt ggcgcgcgaa ccgagcgcgc
     8221 cgacgtagaa ggcggctgat ttcagcgcct ccatcagcgc gaggtcgtcg agcttggggt
     8281 cgtgcgtgag cgccaccagc gcgctgtgcg cgtccagctg caggcgctgc acgaggtcgt
     8341 cgggcatctc gcggctgaac tccgtgccgg gcacgtccca ggtgacgtgg tactcctcgc
     8401 gcgggtcgca gcacagcacc tcgaagtcga gcgcgagcgc catcggcgcc acgacgcgcg
     8461 agagctggcc ggccccgatg atgacgagcc gccagcgcgg cccgaacagc gttcgcatgc
     8521 gctggccgtc gaagctgaag cgctcgccgc gcgtcgcggg ttcgacggcg accgcgccgg
     8581 tggccagctc cagcgtgcgg gcgacgagct cgtggcgcgc gcagcgggcg aggatctcct
     8641 cgatccaggc ggcgtcgcgc agcggctcct gcaccaggcg cagcgtgccg ccgcacggca
     8701 gcccgaagcg cgcggcctcc tcgcgcgtga cgccgtacga cacgagcgcc ggcgccgcga
     8761 cgcgctcgcc gtggcgcacg cggtcgatca ggtcgtcctc gacgcagccg ccggacaccg
     8821 agccgacgac cgggccgtcg tcgcgcagcg cgagcagcgc gccgggcggc cgcggggcgg
     8881 agccccaggt ctcgagcacc gtcaccagcc agacggcgtg gccctgcgcg agccagtcgc
     8941 gcgcct
//
LOCUS       DATCRO010000268         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1049022, whole genome shotgun sequence.
ACCESSION   DATCRO010000268 DATCRO010000000
VERSION     DATCRO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049022"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VIN75_07375"
     CDS             complement(<1..788)
                     /locus_tag="VIN75_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974021.1"
                     /translation="MLFNERLRRIASRSVAGLVAVGALVALASCGGGTYQQQAFVPAR
                     ILSFGDENSRMEGSQGLKYSINGISQSTLQVDCTLNPIWNEVIATSYGLVYQRCNINA
                     NFETNAIDLTTVGATVDDVASSVAAFTAGDTFNGNDLVTLWVGMHDVLNEYKSNATGD
                     ETVLLQDMKTQGIKLASIVNQIEASGAKVIVLTIPDMSDSPFAYTESLKGDFDRMKLL
                     QDMKEQGIKLANLVNQIEASGAKVIVLTIPDMSDSPFAYTESLKG"
     gene            complement(846..1829)
                     /locus_tag="VIN75_07380"
     CDS             complement(846..1829)
                     /locus_tag="VIN75_07380"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8974022.1"
                     /translation="MAIVSGNDPAALQRAAAELAAGELVAFPTETVYGLGARADDDAA
                     VAKIFAAKGRPADHPLIVHVRDVPGAQAFAREWPETARKLADAFWPGPLTVIVPRREG
                     IGTASAAGQDSIGLRCPAHPAAQALLEAAAALGVAGVSGPSANRFGRISPTTAAHVFE
                     EFGDAMTIVDGGECDAGIESAIVDCSRGAPALLRPGVLTRESIEAVLGQPLRQAGRDA
                     PRASGTLEAHYAPRAKLRLMPADQLRVALGVLAKSTVNPVAVYCRTVTPDRSVRASRT
                     MPDDPVAAAHELFASLRELDSTGASLIWVESPPDAPEWDGVRDRLQRAAAA"
     gene            complement(1829..3001)
                     /locus_tag="VIN75_07385"
     CDS             complement(1829..3001)
                     /locus_tag="VIN75_07385"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY8974023.1"
                     /translation="MSGSPTRRALPPGATLGVLGGGQLGRMFVHAAQTLGFDTLVLDP
                     DAHSPAGAAAHAQLCAPYDDERALAEMAGRCDAVTTEFENVPAPSLASLAQDIPVAPS
                     ADAVAICQDRAAEKAHFGRAGVPCAPHAVIETAAQLAAVPDDLLPGILKTARLGYDGK
                     GQVTVVDRAALARAWAQLRNVPCVLEKRLPLAYELSIIVARGADGQVVTLPVQQNLHR
                     GGILALTTVPAPDVTQSQSDQAIARATAIAESMGYVGVLCVEFFVLQDGSLVANEMAP
                     RPHNSGHYSIDACDLSQFELQVRTLAGLPLVTPRQHSPCEMVNLLGDLWFEDGREREP
                     DWGALLALPGMHLHLYGKKQARPGRKMGHLTVTAATPALARERANAAAAVLGLQGF"
     gene            complement(2998..3462)
                     /gene="purE"
                     /locus_tag="VIN75_07390"
     CDS             complement(2998..3462)
                     /gene="purE"
                     /locus_tag="VIN75_07390"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8974024.1"
                     /translation="MGSASDWETMKAAADILAEFAIPFEARVVSAHRMPDDMFAYAEG
                     AVARGLQAIIAGAGGAAHLPGMLAAKTVVPVLGVPVASRHLSGVDSLHSIVQMPKGVP
                     VATFAIGAAGAANAALFAVAQLATRDEALRGRLEAFRAKQTEAARAMTQELK"
     gene            3549..>3806
                     /locus_tag="VIN75_07395"
     CDS             3549..>3806
                     /locus_tag="VIN75_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8974025.1"
                     /translation="MIDITLQNFENDLLQDSMRQPILLDIWAPWCGPCRSLGPILEKL
                     EAAYEGRFKLAKLNSDEQPEIAGQLSQAFGVRSIPFCVMFHQ"
BASE COUNT          529 a         1310 c         1398 g          569 t
ORIGIN      
        1 cccttgagcg attcggtgta ggcgaacggc gagtcgctca tgtcggggat ggtcagcacg
       61 atcaccttgg cgccgctggc ctcgatctgg ttgacgaggt tggccagctt gatgccctgc
      121 tccttcatgt cctgcagcag cttcatgcgg tcgaagtcgc ccttgagcga ttcggtgtag
      181 gcgaacggcg agtcgctcat gtcggggatc gtcagcacga tcaccttggc gccggaagcc
      241 tcgatctggt tgacgatgct ggccagcttg atgccctgcg tcttcatgtc ctgcagcagc
      301 accgtctcgt cgccggtcgc gttggacttg tactcgttga gcacgtcgtg catgccgacc
      361 cacagcgtga cgaggtcgtt gccgttgaag gtgtcgccgg ccgtgaacgc ggccacgctg
      421 ctcgcgacgt cgtcgacggt ggcgccgacg gtggtcaggt cgatcgcgtt ggtctcgaag
      481 ttcgcgttga tgttgcagcg ctggtagacc aggccgtacg aggtcgcgat cacctcgttc
      541 cagatcgggt tcagcgtgca gtcgacctgg agcgtcgact gggagatgcc gttgatgctg
      601 tacttcaggc cctgcgagcc ttccatgcgg ctgttctcgt ccccgaacga caggatgcgc
      661 gccggcacga atgcctgttg ctggtaggtg ccgccaccgc acgaggccag cgcgacgagc
      721 gcgccgacgg cgaccagtcc cgcgaccgat cgcgacgcga tgcggcgcag gcgttcattg
      781 aaaagcattc gttctcctga tggatgttct cggttccggc ggcgcgcgcc gggctccctg
      841 ggttgtcatg cggcggccgc gcgctgcagt cggtcgcgca cgccgtccca ttcgggggca
      901 tcggggggcg actcgaccca gatcagactg gcgcccgtac tgtcgagttc ccgtaaagat
      961 gcgaacagtt cgtgcgcggc ggccaccgga tcgtccggca tcgtgcggct ggcacgcacc
     1021 gagcggtcgg gcgtgacggt gcggcaatat acagcgaccg ggttaacggt cgatttcgcg
     1081 agcacgccca gcgcgacgcg caactggtcg gcgggcatga ggcgcagctt ggcgcgcggc
     1141 gcgtagtgcg cctcgagcgt gcccgaggcg cgcggagcgt cgcggccggc ttggcgcagc
     1201 ggctgtccga gcaccgcctc gatgctttcg cgcgtgagca cgcccggtcg cagcagcgcg
     1261 ggcgcgccgc ggctgcagtc gacgatggcc gactcgatgc cggcgtcgca ctcgccgccg
     1321 tcgacgatgg tcatcgcgtc gccgaactcc tcgaacacgt gcgcggcggt ggtcgggctg
     1381 atgcggccga agcggttggc gctcgggccg gagacgcccg cgacaccgag cgcggccgcc
     1441 gcctcgagca acgcctgcgc ggccggatgc gccgggcagc gcaggccgat ggaatcctgt
     1501 cccgcggccg atgccgtgcc gatgccctcg cggcgtggca cgatcaccgt cagcgggccc
     1561 ggccagaacg cgtcggccag cttgcgcgcg gtctcgggcc actcgcgggc gaacgcctgc
     1621 gcgccgggga cgtcgcgcac gtgcacgatc agcggatggt cggccggccg ccccttggcc
     1681 gcgaagatct tcgcgaccgc ggcgtcgtcg tcggcacgcg cgcccaggcc gtagaccgtc
     1741 tcggtgggga acgcgacgag ctcgcccgcg gccagttcgg ccgcggcgcg ctgcagggcc
     1801 gccgggtcgt tgccgctgac gatggccatc agaacccttg cagccccagc acggccgcgg
     1861 ccgcgttcgc gcgctcgcgg gcgagcgcgg gcgtggcggc cgtcacggtc aggtggccca
     1921 tcttgcggcc cgggcgcgcc tgcttcttgc cgtaaaggtg caggtgcatg ccaggcagcg
     1981 ccaggagcgc gccccagtcg ggctcgcgct cgcggccgtc ctcgaaccac aggtcgccca
     2041 gcaggttgac catctcgcac ggcgagtgct ggcgcggcgt gaccagcggc aggccggcca
     2101 gggtgcgcac ctgcagctcg aactgcgaca ggtcgcacgc gtcgatgctg tagtggccgg
     2161 agttgtgcgg gcgcggcgcc atctcgttgg ccaccagcga gccgtcctgc agcacgaaga
     2221 actcgacgca cagcacgccg acgtagccca tcgactcggc gatggccgtc gcgcgcgcga
     2281 tggcctggtc cgactgcgac tgcgtgacgt cgggcgcggg cacggtggtc agcgcgagga
     2341 tgccgccgcg gtgcaggttc tgctgcaccg gcagcgtgac gacctggccg tcggcgccgc
     2401 gcgccacgat gatgctcagc tcgtaggcga gcggcaggcg cttctcgagc acgcagggga
     2461 cgttgcgcag ctgcgcccag gcccgggcca gcgcggcgcg gtcgaccacg gtgacctggc
     2521 ccttgccgtc gtagcccagg cgggccgtct tgaggatgcc gggcagcagg tcgtcgggca
     2581 cggccgcgag ctgagcggcg gtctcgatga ccgcatgcgg cgcgcacggc acgccggcgc
     2641 ggccgaagtg ggccttctcg gcggcgcggt cctggcagat ggccacggcg tcggccgacg
     2701 gcgcgacggg gatgtcctgc gccagcgagg ccagcgacgg cgccggcacg ttctcgaact
     2761 cggtggtgac ggcgtcgcag cgaccggcca tctcggccag cgcgcgctcg tcgtcgtagg
     2821 gcgcgcacag ctgggcgtgg gcggcggcgc cggcggggct gtgcgcatcg gggtcgagca
     2881 ccagcgtgtc gaagcccagc gtctgcgcgg cgtgcacgaa catgcggccc agctggccgc
     2941 cgccgagcac tcccagcgtg gcgccgggcg gcagcgcgcg gcgggtcggg gaaccgctca
     3001 cttgagctcc tgcgtcatcg cgcgggcggc ctcggtctgc ttcgcgcgga aggcctcgag
     3061 ccggccgcgc agcgcctcgt cgcgcgtggc cagctgcgcc accgcgaaca gggccgcgtt
     3121 ggcggcgccg gcggcgccga tcgcgaacgt ggccacgggc acgcccttgg gcatctggac
     3181 gatggagtgc agcgaatcga cgccgctcag gtggcggctg gccaccggca cgcccagcac
     3241 gggcacgacc gtcttggcgg ccagcatgcc gggcaggtgc gcggcgccgc cggcgcccgc
     3301 gatgatggcc tgcaggccgc gcgcgacggc gccttccgca taggcgaaca tgtcgtcggg
     3361 catgcggtgt gcagagacga cgcgcgcttc gaagggaatg gcgaactcgg cgagaatgtc
     3421 ggcagccgcc ttcatcgtct cccagtcgct ggccgacccc atgacgacgc cgaccacagg
     3481 cgaggcacta ttcaagaatc actcccggta aaacagcgat tttagccggg ggcaaggcag
     3541 agccccacat gatagacatc accctccaga atttcgagaa cgacctgctg caggattcga
     3601 tgcgccagcc catcctcctg gacatctggg cgccgtggtg cggcccgtgc cgcagcctgg
     3661 gcccgatcct ggagaagctc gaggccgcct acgagggccg cttcaagctc gccaagctca
     3721 acagcgacga gcagcccgag atcgccggcc agctgagcca ggccttcggc gtgcgctcca
     3781 tcccgttctg cgtgatgttc caccag
//
LOCUS       DATCRO010000269         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_891813, whole genome shotgun sequence.
ACCESSION   DATCRO010000269 DATCRO010000000
VERSION     DATCRO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891813"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1578)
                     /locus_tag="VIN75_07400"
     CDS             complement(88..1578)
                     /locus_tag="VIN75_07400"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8974026.1"
                     /translation="MDDTERGSAATPWSAIIIGAGFGGLGMAIALRRAGVDDILLLEK
                     GRDVGGVWRENTYPGAACDIPSHLYSFSFEPNPQWTRRYSPQAEILAYLRHCADKYGV
                     REKIRLGCEVASATFDEASASWHVEWTDAAGQPRTGHARALVSAVGLLTRPIIPALPG
                     LADFAGPVFHSAQWNHDVDLRGKRVAVVGTGASAIQFVPELARIASRLTVFQRTPPWI
                     LPRGDRGYSEAERRRFTRWPWLMAAHRAWLYVQHDGRALGFTRLPWLMDIVGAAPARK
                     LLRRQVADPGLRDRLTPRYTIGCKRVLISDDWLPTFAQPHVELVTDAIEAVTPAGVRV
                     AGGREVEADAIVLGTGFAAHQFLAPMTIRGRGGRTLDEAWADGAKAWLGISVPAFPNF
                     FMLYGPNTNLGHSSIVFMLECQVAHVMRCLRRRGDARGSAIEVRSAPFERYNAKLQRR
                     IRGTVFTGCGNWYVDPQGRHTVNWPGFTTGYRWLTRHAGLSAYEVR"
     gene            complement(1752..2768)
                     /locus_tag="VIN75_07405"
     CDS             complement(1752..2768)
                     /locus_tag="VIN75_07405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8974027.1"
                     /translation="MPSLSSPKLPPLSILDLVPVQQGSTPGEALRNSLALAKHAEQLG
                     YTRYWVAEHHNMTGIASAATSVVIGFLAQGTSKIRIGSGGIMLPNHSPLVIAEQFGTL
                     ASLYPGRIDCGLGRAPGTDPTTARALRRDPRAADTFPEDVQELQALFQPVRPGQAVQA
                     FPGAGLDVPVWILGSSTYGAQLAAALGLPYAFASHFAPDALTQAMELYRHLFKPSEQL
                     RAPYAMAGVNVVVADTDAEARRLFTTHQQSFANLRRGRPGPQQPPIDDIDAYWSPAEK
                     QMAESMLACSIVGSPDTVRHGLERFAAYSGGDELMVVSAIFDPAARRKSYSMLAQIAG
                     LAPR"
     gene            3048..3725
                     /locus_tag="VIN75_07410"
     CDS             3048..3725
                     /locus_tag="VIN75_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8974028.1"
                     /translation="MPRPTRLQYPGALYHVTARGVQQGTIFLDDLDRGALLKYIARAL
                     EEGNARAFAYCLMGNHYHLVLQTHQSNLSKLMQRINSGFCQSVNRRHQRCGHLLEGRF
                     KAIVVDRDSHLLEACRYVDLNPVRAGLVASPLQWRWSSYAAHVGRAPVPAWLATDELH
                     GVLTGRATPSCDALRTARRRYADWVAAGRGVRLWDESLHHGLYLGDQAFVERMRGKVG
                     PGDRDQA"
BASE COUNT          562 a         1284 c         1347 g          534 t
ORIGIN      
        1 cgcgatggcc tcggcgttcg accacgagca cgtgtgggcg tcgacggcgc ggttgcgcgc
       61 gcgcgagcgc gaacgcgcgg cgcaggctca gcgcacctcg taggccgaca gcccggcgtg
      121 ccgcgtgagc cagcggtagc ctgtggtgaa gcccggccag ttgaccgtgt ggcggccctg
      181 cgggtcgacg taccagttgc cgcagccggt gaacaccgtg ccccggatgc gccgctgcaa
      241 cttcgcgttg tagcgctcga acggcgcgct cctcacctcg atggcgctgc cgcgcgcgtc
      301 gccgcgcctg cgcaggcagc gcatcacgtg cgcgacctgg cactcgagca tgaagacgat
      361 cgagctgtgg ccgaggttgg tgttggggcc gtacagcatg aagaagttcg ggaaggcggg
      421 gacgctgatg cccagccagg ccttggcgcc gtccgcccag gcctcgtcga gcgtgcgtcc
      481 gccgcggccg cggatcgtca tcggcgcgag gaactggtgc gcggcgaagc ccgtgcccag
      541 cacgatggcg tcggcctcga cctcgcgccc gcccgcgacg cgcacgcccg cgggcgtgac
      601 ggcttcgatg gcgtcggtga cgagttcgac atggggctgg gcgaacgtcg gcagccagtc
      661 gtcggagatc agcacgcgct tgcagccgat cgtgtagcgc ggcgtcaggc gatcgcgcag
      721 cccggggtcg gcgacctgcc ggcgcagcag cttgcgcgcg ggcgcggccc cgacgatgtc
      781 catcagccag ggcaggcgcg tgaagcccag cgcacggccg tcgtgctgca cgtacagcca
      841 cgcgcgatgc gccgccatca gccacggcca gcgcgtgaag cgccggcgct cggcctccga
      901 atagccgcgg tcgccgcgtg gcaggatcca tggcggcgtg cgctggaaca ccgtcagccg
      961 cgacgcgatg cgcgccagct ccggcacgaa ctggatcgcc gacgccccgg tgccgacgac
     1021 cgcgacgcgc ttgccgcgca ggtcgacgtc gtggttccac tgcgccgaat ggaacacggg
     1081 gccggcgaag tcggccaggc cgggcaacgc cgggatgatc ggccgcgtca gcagcccgac
     1141 ggcgctcacg agcgcgcggg cgtggccggt gcgcggctgc ccggccgcgt ccgtccactc
     1201 gacgtgccac gacgcactcg cctcgtcgaa cgtggccgag gcgacttcgc agccgaggcg
     1261 gatcttctcg cgcacgccgt acttgtcggc acagtggcgc aggtaggcaa ggatctcggc
     1321 ctgcggcgaa taccggcgcg tccattgcgg gttgggctcg aacgagaacg agtacaggtg
     1381 cgacgggatg tcgcaggccg cgcccgggta ggtgttctcg cgccagacgc cgccgacgtc
     1441 gcggcccttc tccagcagca ggatgtcgtc cacgcccgca cgccgcagcg cgatggccat
     1501 gcccaggccg ccgaagccgg cgccgatgat gatggcgctc cagggcgtgg cggcgctgcc
     1561 gcgttcggtg tcgtccatgg agcggacttt aggccgcgcg atgcagggag cggggcgtgg
     1621 atatcccgca gcccggtcgc gacgccggcc aatggcgggt tacggctgcg gctgacccgc
     1681 cctgcggcgc acggcttttg cagcgcgggt tggccgaagg cgcgacccgc caaaacgaac
     1741 gcagcgcggc atcatcgcgg cgcgagaccc gcgatctgcg ccagcatgct gtacgacttc
     1801 cggcgcgctg ccgggtcgaa gatcgccgac accaccatca gctcgtcgcc accgctgtac
     1861 gccgcgaagc gctcgagccc gtggcgcacg gtgtcgggcg agccgacgat ggagcacgcg
     1921 agcatgctct cggccatctg cttctcggcg ggcgaccagt acgcgtcgat gtcgtcgatc
     1981 ggtggctgct gcggccccgg ccggccgcgg cgcaggttcg cgaacgattg ctggtgcgtg
     2041 gtgaacaggc gtctcgcctc ggcgtcggtg tcggccacga ccacgttgac gccggccatc
     2101 gcatacggcg cgcgcaactg ctccgacggc ttgaacaggt ggcggtagag ctccatcgcc
     2161 tgtgtgagcg cgtcgggcgc gaagtgcgac gcgaacgcgt acggcaggcc cagcgcggcg
     2221 gccagttgcg cgccataggt gctcgagccg aggatccaga ccggcacgtc caaccccgcg
     2281 ccgggaaagg cctgcaccgc ctggcccggc cgcacgggct ggaacagcgc ctgcaattcc
     2341 tgcacgtcct cggggaaggt gtcggccgcg cgcgggtcgc gccgcagcgc ccgcgccgtc
     2401 gtcggatcgg tgccgggcgc gcggccgagg ccgcagtcga tgcggccggg gtacagcgac
     2461 gccagcgtgc cgaactgctc ggcgatgacc agcggcgagt ggttgggcag catgatgccg
     2521 cccgagccga tgcggatctt cgacgttccc tgggcaagaa agccgatcac caccgacgtc
     2581 gccgcgctgg cgatgcccgt catgttgtgg tgctccgcca cccagtagcg cgtgtagccc
     2641 agctgctcgg cgtgctttgc gagcgccagg ctgttgcgca gcgcctcgcc gggcgtcgaa
     2701 ccttgctgca cggggacgag atcgaggatg gacagcggcg ggagcttggg ggacgagagc
     2761 gagggcatca ggggcgggga tcggaagcgg ggttggaggc gataccttaa ggggaaatcc
     2821 gagaccgagg tggtggtggg gaggaattgg gcagcgacgc accgaagccg gaggggtctg
     2881 gcctatccac cgtccgcgct gcagcgctga tcagggcggc tgcggcgttc gcggacgggg
     2941 gccaggcctg acctcgatcc cacgagatct ctcaccggcg cattcggtgc cgaaaatcgg
     3001 gcgatccgcg caccccgacg gtctggcaag tccgctagcc tgacgccatg ccccgcccca
     3061 cccgtctcca atatcctggc gccctctacc acgtcaccgc gcgcggtgtg cagcaaggca
     3121 ccatcttcct ggacgatctc gatcgaggag ccttgctcaa gtacatcgcc cgcgccctgg
     3181 aggaaggaaa cgcccgcgca ttcgcctatt gcctcatggg caaccactac cacctcgtcc
     3241 tccagacgca ccagtcgaac ctgtccaagc tgatgcaacg catcaacagc ggcttctgcc
     3301 aatccgtcaa ccgacgccac cagcgctgcg gccacctgct ggagggccgg ttcaaggcca
     3361 tcgtcgtgga tcgcgacagc catctgctcg aagcgtgtcg ctacgtggat ttgaaccccg
     3421 tgcgtgctgg cttggttgca tcgccattgc aatggcgatg gtccagctac gcggcacatg
     3481 ttggccgcgc accggtcccg gcgtggctcg ccacggacga actgcatggc gtgttgaccg
     3541 gacgcgcgac cccttcgtgc gacgcgcttc gaacggctcg tcgacgctac gccgattggg
     3601 tcgctgcggg acgcggcgtg cgactctggg acgagtcgct gcatcacggt ctctacctcg
     3661 gcgaccaagc cttcgtcgag cgaatgaggg gcaaggttgg cccgggggat cgggatcagg
     3721 cctgacc
//
LOCUS       DATCRO010000270         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_14330, whole genome shotgun sequence.
ACCESSION   DATCRO010000270 DATCRO010000000
VERSION     DATCRO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14330"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VIN75_07415"
     CDS             complement(<1..311)
                     /locus_tag="VIN75_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974029.1"
                     /translation="MNARLRRYHPLRAERHGRHTALAIALLALGAGLTGCASRRPPPE
                     PPAVVAPAMAPLPPAPPLDVRDGHVWSPDIAAVARELQQLALDNGQVEVMRTAGNQVR
                     VR"
     gene            complement(405..1727)
                     /gene="sctN"
                     /locus_tag="VIN75_07420"
     CDS             complement(405..1727)
                     /gene="sctN"
                     /locus_tag="VIN75_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HEY8974030.1"
                     /translation="MKALQGFTDLMEAELASATMVGRTGKVTEVIGTLMRVSGLDARL
                     GELCEVLDDDGGVLQTAEVVGFAGGRSILSPFGSILGVRGGTRVVGLGQVLSVPVGEA
                     LLGRVIDSLGRPIDGKGPVRTTESRPVFALPPTPMEREMIERPLPTGVKVIDGLITLG
                     EGQRMGIFAPAGVGKSTLMGMLARGTQCDISVIALIGERGREVREFIELIMGEAGMAR
                     SVVVCATSDRSSSERAKAAHVATAVAEYYRDQGLKVLLMMDSLTRFARAQREIGLAAG
                     EPPARRGFPPSVFAEIPRLLERAGMGARGSITALYTVLYEDEGGNDPISEEVRGILDG
                     HMILSRKIAARNQYPAIDVLGSLSRVMSQIVPKSHQAAAGHLRALMAKYDEIEPLVQM
                     GEYKPGNDALADEAMRKLQPVREFLCQATDDLWAFDRTLGSLGQVVAG"
     gene            complement(1741..2640)
                     /gene="sctL"
                     /locus_tag="VIN75_07425"
     CDS             complement(1741..2640)
                     /gene="sctL"
                     /locus_tag="VIN75_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system stator protein SctL"
                     /protein_id="HEY8974031.1"
                     /translation="MIWLQGGAGADRLGGLGAEDGILRAAELDGAAELDALRRAMEEQ
                     RDAVLAQARGQALALVNDARDEAARLLAAARADAERLRGEACEAGRRQAALEWHERQT
                     VQAIDNAQVLRGMHEKLATIVTGAVERIVHTEERAALYQRALRSVRSFARGASTLALR
                     VSADDHAHARAALDSLKALQTQGLHIEVSVDPSLRAGSCVFESEVGILDASLQTQLDG
                     LRAAMDRAVRKALSSSLPLADADDQEAPEHGLDDGPGDGIGDRGIEAQAHASFGFPHT
                     DADARADADDAEDILHAHDLAEH"
BASE COUNT          347 a         1029 c          955 g          384 t
ORIGIN      
        1 cgcacgcgca cctggttgcc ggccgtgcgc atcacctcca cctggccgtt gtccagcgcc
       61 agctgctgca gctcccgcgc cactgccgcg atgtcgggtg accagacgtg gccgtccctg
      121 acgtcgagcg gaggggccgg cggcaacggc gccatcgccg gcgcgacgac ggccggcggt
      181 tccggcggtg ggcggcgcga ggcgcagccg gtcaggccgg cgcccagcgc gagcagcgcg
      241 atcgcgagcg cggtgtgccg gccgtggcgc tcggcgcgaa gggggtgata gcggcgaagg
      301 cgtgcattca tcgggtcact ccggatcgtt gaaacggccc ggccccagga ccagcggggc
      361 agggcgcgcg atcggcctgc gcgtcgcgga cgcgcgtgcc ttcctcatcc cgccacaacc
      421 tggcccagcg accccagcgt gcggtcgaag gcccagaggt cgtcggtggc ctggcacagg
      481 aactcgcgca ccggctgcag cttgcgcatg gcctcgtcgg cgagcgcgtc gttgccgggc
      541 ttgtactcgc ccatctgcac cagcggctcg atctcgtcgt acttggccat cagcgcccgc
      601 aggtggcccg cggccgcctg atgcgacttg ggaacgatct gcgacatcac ccgcgacagg
      661 ctgcccagca cgtcgatcgc cgggtactgg ttccgcgcgg cgatcttgcg cgagaggatc
      721 atgtggccgt ccaggatgcc gcgcacctcc tcggagatcg ggtcgttgcc gccctcgtcc
      781 tcgtacagga cggtgtagag cgcggtgatc gatccgcgcg cgcccatgcc ggcgcgctcg
      841 agcagccgcg ggatctcggc gaacaccgac ggcggaaagc cgcgccgcgc cggcggctcg
      901 cccgccgcca ggccgatctc gcgctgcgcc cgcgcgaagc gggtcagcga gtccatcatc
      961 agcagcacct tcaggccctg gtcgcggtag tactcggcca ccgcggtggc gacatgcgcc
     1021 gccttggcgc gctcgctcga cgagcggtcc gaggtcgcgc acaccaccac cgagcgcgcc
     1081 atgccggcct cgcccatgat gagctcgatg aactcgcgca cctcgcggcc gcgctcgccg
     1141 atcagcgcga tgacgctgat gtcgcactgc gtgccgcgcg ccagcatgcc catcagggtg
     1201 gacttgccga cgccggcggg cgcgaagatg cccatccgct gaccttcgcc caaggtgatc
     1261 aggccgtcga tcaccttgac cccggtcggc agcgggcgct cgatcatctc gcgctccatc
     1321 ggcgtgggcg gcagcgcgaa caccgggcgc gattcggtcg tgcgcaccgg gcccttgccg
     1381 tcgatcggcc ggcccaggct gtcgatcacg cggcccagca gcgcctcgcc gaccggcacc
     1441 gacagcacct ggcccaggcc gacgacgcgg gtgccgccgc gcacgcccag gatcgatccg
     1501 aacggcgaga ggatgctgcg cccgccggcg aagcccacca cctcggccgt ctgcagcacg
     1561 ccgccgtcgt cgtcgagcac ctcgcacaac tcgcccagcc gcgcgtccag gccgctcacc
     1621 cgcatcagcg tgccgatcac ctcggtgacc ttgccggtgc gtcccaccat cgtggccgag
     1681 gccagctcgg cctccatcag gtcggtgaat ccctgcaatg ccttcatcgg cgctcctggt
     1741 tcagtgttcg gcgaggtcgt gcgcgtgaag gatgtcctcg gcgtcatcgg catcggcccg
     1801 cgcatccgcg tccgtgtgcg ggaatccgaa ggacgcgtgg gcctgggcct cgatgccgcg
     1861 atcgccgatg ccgtcgcccg gtccgtcatc gaggccgtgt tcgggtgctt cttggtcgtc
     1921 ggcatcggcc agcggcaggc tgctggacag cgccttgcgc accgcgcggt ccatcgccgc
     1981 gcgcaggccg tcgagttgcg tctgcaggct cgcatcgagg atgccgacct ccgactcgaa
     2041 cacgcagctg cccgcccgca gcgacgggtc gacgctgacc tcgatgtgaa gcccctgggt
     2101 ctgcagcgcc ttgagcgaat ccagcgcggc gcgcgcatgg gcatggtcgt cggcgctgac
     2161 gcgcagggcc agcgtgctgg cgccgcgcgc gaagctgcgc acgctgcgca gcgcgcgctg
     2221 gtacagcgcc gcccgttcct cggtgtggac gatgcgctcg acggcgccgg tgacgatggt
     2281 cgccagcttc tcgtgcatgc cgcgcagcac ctgcgcgttg tcgatcgcct gcaccgtctg
     2341 gcgctcatgc cattcgagcg ccgcctggcg ccggccggct tcgcaggcct cgccccgcag
     2401 gcgctcggcg tcggcgcgcg cggcggcgag caggcgggcg gcctcgtcgc gcgcgtcgtt
     2461 caccagcgcc agcgcctggc cgcgggcctg cgccaggacc gcatcgcgct gctcctccat
     2521 cgcgcggcgc agcgcgtcga gttccgccgc gccgtcgagc tcggcggcgc gcaggatgcc
     2581 gtcctcggcg ccgagtccgc cgagacggtc cgcgccagcg ccgccctgca gccagatcat
     2641 catgtcggtt ccttcgcgaa gagcgcgtcc aggcgggcca ggccctccag cgcgcaggcg
     2701 tgacggaccg gcacc
//
LOCUS       DATCRO010000271         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_396748, whole genome shotgun sequence.
ACCESSION   DATCRO010000271 DATCRO010000000
VERSION     DATCRO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396748"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VIN75_07430"
     CDS             <1..1091
                     /locus_tag="VIN75_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974032.1"
                     /translation="PLLPIAAAAQTLQKNVAVPPELSELLPMIRRNVLLEARLIEDLL
                     DLTAISEGKLSLRRAPVDMRKIIDAVVEMVQETVDEKQIDLAVHWNARRGIVDGDAAR
                     LQQVLWNLVRNAVKFTPAGGHIRIAVDDDADDARLRLRCIDDGIGIEPGALGTIFSAF
                     EQAGDDVARQFGGLGLGLAIAQALVQGHGGELSASSEGPGRGATFTLRLDTIEAPDVA
                     ADRPPAPAPHSGASRPLRMLLVEDNEDAACALSMSLEFMGYEVTHAANLRAAMAAGQG
                     GAFDVIVTDLGLPDGSGLALGPALSARVPLIALSGFGSAEDVARSMAAGFAAHLVKPT
                     DPDDVHATVGKILMRAMAAPASMGAGQA"
     gene            complement(1190..2797)
                     /gene="rmuC"
                     /locus_tag="VIN75_07435"
     CDS             complement(1190..2797)
                     /gene="rmuC"
                     /locus_tag="VIN75_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8974033.1"
                     /translation="MNPASPTPVIVFAVLFVLGAVACVAVSLLLARRGQGTPDAAAAE
                     AGHAAQLELAQLRERLRATEQAAAALQADLERSRGEMRAAREELATAQETVLRAAAGE
                     AQKNQQVASLHERVADLEQKARDTAAQLDARNVALQAAHAELAHARTRADAERASAQE
                     KLQLLTEARTELSNQFKALASEILEEKAKRFAEQNQQNLGTLLDPLRMQLNEFKGKVE
                     EVYVKEGQERFALTEQVKQLMELNKTLSNDANNLTKALKGQAKTQGNWGELILERVLE
                     ASGLEKGRVFHVQVSQRDAEGNLLQPDVVIDLPQDRKIVVDAKVSLVAYDQYANAESD
                     ESRLLSARAHLDSVRAHIKGLSAKNYQALYGVKSLDMVVMFVPIEPAFMLAVTSDESL
                     FMEAWQRNVLLVSPSTLLFVVRTIAHLWRQEAQTRNAQDIAKRGAELYDKLAAFVGDL
                     QKVGAKLKDAQSAYEDAEKRLSTGKGNVIRQAEMLKDLGVKSTKALPGTLVDASGARD
                     DVAPPPAGLPPSLSLFDEAPDPEAPLN"
     gene            2897..3202
                     /locus_tag="VIN75_07440"
     CDS             2897..3202
                     /locus_tag="VIN75_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8974034.1"
                     /translation="MGYAVVHLFLRDPPAKNAAPAPAPAPTPAADPAPPHWTTVLALP
                     PDASVEQIREAYRRLISQYHPDKVASLGRELQALAEAKSKEIALAYQDALMPRGGAR"
     gene            complement(3203..>3545)
                     /locus_tag="VIN75_07445"
     CDS             complement(3203..>3545)
                     /locus_tag="VIN75_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY8974035.1"
                     /translation="PETLDNLLEEITDRFGKLPPQGQTLFDTHRLRLLAKSYGVIKVD
                     ANPKVMNVTFRPNPPIDPMRIIELVQKNKSVKLAGNDRLRIDREIAEPRDRAQYVRDL
                     LRSLGPPTVAA"
BASE COUNT          454 a         1253 c         1282 g          556 t
ORIGIN      
        1 cgccgctgct gccgatcgcc gcggcggccc agacgctgca gaagaacgtc gcggtgccgc
       61 cggagctgtc ggagctgctg ccgatgatcc ggcgcaacgt gctgctggag gctcgcctga
      121 tcgaggacct gctcgatctg actgcgattt ccgagggcaa gctgtcgctg cggcgcgcgc
      181 cggtggacat gcgcaagatc atcgacgcgg tcgtcgagat ggtgcaggag accgtcgacg
      241 agaagcagat cgacctcgcg gtgcactgga acgcccgtcg cggcatcgtc gacggcgacg
      301 ccgcgcggct gcagcaggtg ctgtggaacc tggtgcgcaa cgcggtcaag ttcacgccgg
      361 ccggcggcca catccgcatc gcggtcgacg acgacgccga cgacgcgcgg ctgcgcctgc
      421 gctgcatcga cgacggcatc gggatcgagc cgggcgcgct ggggaccatc ttctcggcgt
      481 tcgagcaggc cggcgacgac gtcgcgcgcc agttcggcgg cctgggcctg ggcctggcga
      541 tcgcgcaggc gctggtgcag gggcacggcg gcgagctgtc cgccagcagc gaggggccgg
      601 gccgcggcgc cacgttcacg ctgcggctgg acaccatcga ggcgcccgac gtcgccgccg
      661 accggccgcc cgcccccgcg ccccacagcg gcgcgtcgcg cccgctgcgc atgctgctgg
      721 tcgaggacaa cgaggacgcc gcctgcgccc tgtcgatgtc gctcgagttc atgggctacg
      781 aggtcacgca cgcggccaac ctgcgcgccg cgatggccgc ggggcagggc ggcgcgttcg
      841 acgtgatcgt cacggacctg ggcctgcccg acggctcggg cctggccctg ggcccggcgc
      901 tgtcggcgcg cgtcccgctg atcgcgctga gcggcttcgg cagcgccgag gacgtggccc
      961 gctcgatggc cgccggcttc gccgcgcatc tggtcaagcc gaccgatccg gacgacgtgc
     1021 atgcgaccgt gggcaagatc ctgatgcgcg cgatggccgc gccggcgtcg atgggcgcgg
     1081 ggcaggccta gggcgaggcg ttcgaaaccg gcgtcaggca tctgccgcgc atcgccgccg
     1141 ggacgacgat gacgatgcct gacgctgccc gaggccgatc gctgcgcctt cagttcagcg
     1201 gcgcctcggg atccggcgct tcgtcgaaca gcgacaacga tggcggcagg ccggcaggcg
     1261 gcggggccac gtcgtcgcgc gcgcccgagg cgtcgaccag cgtgccgggc agcgccttgg
     1321 tggacttcac cccgagatcc ttgagcatct ccgcctggcg gatgacgttg cccttgccgg
     1381 tggacaggcg cttctccgcg tcctcgtacg ccgactgcgc gtccttgagc ttggcgccca
     1441 ccttctgcag gtcgccgacg aacgcggcga gcttgtcgta gagctcggcg ccgcgcttgg
     1501 cgatgtcctg cgcgttgcgc gtctgcgcct cctggcgcca caggtgggcg atggtgcgca
     1561 cgacgaacag cagcgtcgac gggctgacca gcagcacgtt gcgctgccag gcctccatga
     1621 acagcgattc gtcgctggtg accgccagca tgaacgccgg ctcgatcggc acgaacatca
     1681 ccaccatgtc gagcgacttc acgccgtaca gcgcctggta gttcttcgcc gacaggccct
     1741 tgatgtgcgc gcgcaccgaa tcgaggtggg cccgcgcgga gagcaaacgg ctctcgtcgg
     1801 attcggcgtt ggcgtactgg tcgtaggcga ccagtgacac cttggcgtcg accacgatct
     1861 tgcgatcctg cggcaggtcg atcacgacgt cgggctgcag caggttgcct tcggcgtcgc
     1921 gctggctcac ctgcacatgg aagacccggc ccttctcgag gccggaggcc tccagcacgc
     1981 gctccaggat cagctcgccc cagttgccct gcgtcttggc ctggcccttg agcgccttgg
     2041 tgaggttgtt ggcgtcgttg ctgagggtct tgttcagctc catcagctgc ttgacctgct
     2101 cggtcagcgc gaagcgctcc tggccttctt tgacgtagac ctcctcgacc ttgcccttga
     2161 actcgttcag ctgcatgcgc agcggatcga gcagggtacc caggttctgc tggttctgct
     2221 cggcgaagcg cttggccttc tcctcgagga tctcgctcgc cagcgccttg aactggttgg
     2281 acagctcggt cctggcctcg gtgagcagtt gcagcttctc ctgcgcgctc gcgcgctccg
     2341 cgtcggcgcg cgtgcgcgcg tgggcgagct cggcgtgcgc cgcctgcagt gcgacgttgc
     2401 gggcgtcgag ctgggcggcg gtgtcgcgcg ccttctgctc gaggtcggcg acgcgctcgt
     2461 gcagcgacgc gacctgctgg ttcttctgcg cctcgccggc ggcggcgcgc agcaccgtct
     2521 cctgggccgt ggccagctcc tcgcgggccg cgcgcatctc gccgcggctg cgttcgaggt
     2581 cggcctgcaa cgcggccgcg gcctgctcgg tggcgcgcag gcgctcgcgc aactgggcca
     2641 gctccagctg cgcggcgtgg ccggcctcgg ccgccgcggc gtcgggcgtc ccctggccgc
     2701 ggcgggccag cagcaggctc acggccacgc aggcgacggc tccgaggacg aacagcacgg
     2761 caaagacgat gacgggagtg ggggaggccg gattcatgcg gcgattgtcg ccgctccact
     2821 ggctcacggc atgcgccacc ggtgggcttc cgggccgcca gggcgagggt tttcgagggc
     2881 cggcgctggc gccctcgtgg gctacgccgt ggtgcacctg ttcctgcgcg acccgcccgc
     2941 caagaacgcc gcgcccgcgc ccgcgcccgc accgacgccc gcggccgacc ctgcgccgcc
     3001 gcactggacg acggtgctgg cgctgccgcc cgacgcgagc gtcgagcaga tccgcgaggc
     3061 ctaccggcgc ctgatcagcc agtaccaccc cgacaaggtg gcctcgctgg gccgcgagct
     3121 gcaggcgctg gccgaggcca agtccaagga gatcgcgctc gcctaccagg acgcgctgat
     3181 gccgcgcggc ggcgcccgct gatcaggccg ccaccgtcgg cggacccagc gagcgcagca
     3241 ggtcgcgcac gtactgcgcg cggtcgcgcg gctcggcgat ctcgcggtcg atgcgcaggc
     3301 ggtcgttgcc ggccagcttc acgctcttgt tcttctgcac cagctcgatg atgcgcatcg
     3361 ggtcgatcgg cggattgggg cggaaggtga cgttcatcac cttcgggttg gcatcgacct
     3421 tgatcacgcc gtagctcttg gccagcagcc gcaggcggtg cgtgtcgaac agcgtctggc
     3481 cctgcggcgg gagcttgccg aaacggtcgg tgatctcctc gagcaggttg tccagcgtct
     3541 ccggg
//
LOCUS       DATCRO010000272         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_362806, whole genome shotgun sequence.
ACCESSION   DATCRO010000272 DATCRO010000000
VERSION     DATCRO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362806"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="VIN75_07450"
     CDS             <1..1616
                     /locus_tag="VIN75_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY8974036.1"
                     /translation="LGLTNGGEPVRRFGASLLTDPAVGASATDPLPTVPADYVIKPGD
                     EVAVTIWGALDADLRLTVSRAGTISIPRVGAINVGGLHYSELAPAITRQVGQQFHNFQ
                     LTATMGAVRAIRVFVNGYARRPGSIAVNGLSSLLHVIMRAGGPSAAGSFRDVRLNRGG
                     KEIARFDLYDVLLKDDRRVDTLVQPDDIVYIGPIGAQVAVLGSVNQQAIFELKPGETL
                     ADALQMAGGFTSVADASRVSIERLADRHGGRVRDLPLPQHAGDTVSNGDVVWAYSAVS
                     AALPKLKQNERIHIEGEVAMPGDYVLPPGSTSADAVRVAGGLTQAAYPFGTEFTRRSV
                     RKTQQANYDRAIRELETSMAKTSTTRVSTTEEATARAASAASNQRLLERLRELKPSGR
                     IVLEIDPEATALPDFPLEDGDSITVPGRDSSVGVFGSVFNAGSFAFKPSRTTEQYLHL
                     AGGPTRGADKHSMFLIRANGSVVSALQGSSFWHSGNEFRDAIIYPGDAIFVPEELDKS
                     TFVQDAKDWTQILYQFGLGIAGINALNIR"
     gene            1613..2800
                     /locus_tag="VIN75_07455"
     CDS             1613..2800
                     /locus_tag="VIN75_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY8974037.1"
                     /translation="MTDPHLGAAPAADPAAAAPAPAAPRRADIVDLLLPLTSRWRSLL
                     VVPVVAALLAAGATFLVKPTYSSSVTFLPPQQQNTAASALSSLGALAGISTGSGKSSA
                     DQMVSLMTSIDATDRLIDKYHLLEVYDKRWREQARIELGKHSDFRIGKKDGLITVSVE
                     DTDRARAAAMANDYVAELRRMTSALAVSEAQQRRMFFEAQLKQTKDRLVAAQLALGQS
                     GFDIGALKSEPKAAAETYANLRAQLTTAQVKLQMLRSSRSETNVEVLQQKAVVDELQQ
                     KTDALEASTKPDASNQPDYVGKYREFRYQETLFDQMARQYELARIDEAKEGALIQVVD
                     PARPAERKSGPRRLRIAIEAGVAALLLFALGLVVRDRWRMAAGDPHVAQRLRVLRDSL
                     RRH"
     gene            3164..>3758
                     /locus_tag="VIN75_07460"
     CDS             3164..>3758
                     /locus_tag="VIN75_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEY8974038.1"
                     /translation="MKKIVSVVGARPQFVKAAVVSEQLRRVGVQEVMIHTGQHYDANM
                     SDAFFDALGIPAPRYNLGIGGGAHGEMTGRQLIEIERRLMDEKPQAVLVYGDTNSTLA
                     GALAAVKLHIPIAHVEAGLRSFNTRMPEEINRIVTDRISAVLFTPSRLASDNLRAEGV
                     ADSRIVEVGDVMYDAALRYGNAPAAGGNGRPFFLVTIH"
BASE COUNT          576 a         1438 c         1256 g          488 t
ORIGIN      
        1 gcctgggcct gaccaacggc ggcgagccgg tgcgccgctt cggcgcgtcg ctgctgaccg
       61 acccggccgt cggtgccagc gccaccgacc cgctgcccac ggtgccggcc gactacgtga
      121 tcaagccggg cgacgaggtc gcggtgacga tctggggcgc gctcgacgcc gacctgcgcc
      181 tgacggtgtc gcgcgccggc acgatctcga tcccgcgcgt cggcgcgatc aacgtcggcg
      241 gcctgcacta ctcggagctg gccccggcca tcacgcgcca ggtcggccag cagttccaca
      301 acttccagct gaccgccacg atgggcgcgg tgcgcgcgat ccgcgtgttc gtcaacggct
      361 acgcgcggcg ccccggcagc atcgcggtca acgggttgtc gtcgctgctg cacgtcatca
      421 tgcgcgccgg cggcccgtcg gcggccggca gcttccgcga cgtccgcctg aaccgcggcg
      481 gcaaggagat cgcgcgcttc gacctctacg acgtgctgct caaggacgac cgccgcgtcg
      541 acacgctggt gcagccggac gacatcgtct acatcggccc gatcggcgcg caggtggcgg
      601 tgctgggcag cgtgaaccag caggcgatct tcgagctcaa gccgggcgag acgctggccg
      661 acgcgctgca gatggcgggc ggcttcacgt ccgtggccga cgccagccgc gtctccatcg
      721 aacgcctggc cgaccggcac ggcggccgcg tgcgcgacct gccgctgccc cagcacgccg
      781 gcgacacggt ctccaacggc gacgtcgtct gggcctacag cgcggtgagc gccgcgctgc
      841 ccaagctcaa gcagaacgag cgcatccaca tcgagggcga ggtcgccatg ccaggcgact
      901 acgtcctgcc gccgggcagc acctcggccg acgccgtgcg cgtggccggc ggcctgacgc
      961 aggcggccta tccgttcggc accgagttca cgcgccgctc cgtgcgcaag acccagcagg
     1021 ccaactacga ccgcgcgatc cgcgagctcg agacgtcgat ggccaagacc tccacgacgc
     1081 gcgtgtcgac caccgaggaa gcgaccgcgc gcgcggcgtc ggccgcctcc aaccagcggc
     1141 tgctcgaacg cctgcgcgag ctcaagccca gcggccgcat cgtgctggag atcgatcccg
     1201 aagcgacggc cctgcccgat ttcccgctcg aggacggcga cagcatcacc gtgcccggcc
     1261 gcgactcgtc ggtcggcgtg ttcggcagcg tcttcaacgc cggcagcttc gcgttcaagc
     1321 cgagccgcac caccgagcag tacctgcacc tggccggcgg cccgacgcgc ggcgccgaca
     1381 agcacagcat gttcctgatc cgcgccaacg gctcggtcgt gagcgccctg cagggcagct
     1441 cgttctggca ctcgggcaac gagttccgcg acgccatcat ctatccgggc gacgccatct
     1501 tcgtgccgga ggaactggac aagtccacct tcgtgcagga cgccaaggac tggacgcaga
     1561 tcctgtacca gttcgggctc ggcatcgccg gcatcaacgc actgaacatc cgatgaccga
     1621 tccgcacctc ggcgccgccc ccgccgccga ccccgccgcg gcggcgcccg cgccggccgc
     1681 gccgcgccgg gccgacatcg tcgacctgct gctgccgctg acctcgcgct ggcgctcgct
     1741 gctggtcgtg cccgtcgtgg ccgcccttct cgccgccggc gccacgttcc tcgtgaagcc
     1801 gacgtactcg tcgagcgtca ccttcctgcc gccgcagcag cagaacacgg ccgcctcggc
     1861 gctgtcgtcg ctcggcgcgc tggcgggcat ctccacgggc agcggcaaga gctcggccga
     1921 ccagatggtc tcgctgatga cgagcatcga cgcgaccgac cgcctgatcg acaagtacca
     1981 cctgctcgag gtctacgaca agcgctggcg cgagcaggcc cgcatcgagc tgggcaagca
     2041 ctccgacttc cgcatcggca agaaggacgg cctgatcacg gtgtcggtcg aggacaccga
     2101 ccgcgcgcgc gccgcggcca tggccaacga ctacgtcgcc gagctgcgcc gcatgacctc
     2161 cgcgctggcg gtcagcgagg cccagcagcg ccgcatgttc ttcgaggcgc agctcaagca
     2221 gaccaaggac cgcctggtcg ccgcgcagct ggcgctcggc cagagcggct tcgacatcgg
     2281 cgcactgaaa tccgagccca aggccgccgc cgagacctat gccaacctgc gcgcgcagct
     2341 gacgaccgcg caggtcaagc tgcagatgct gcgcagctcg cgctccgaga cgaacgtcga
     2401 ggtgctgcag cagaaggcgg tcgtcgacga gctgcagcag aagacggacg cgctcgaggc
     2461 ctcgaccaag ccggacgcgt cgaaccagcc cgactacgtc ggcaagtacc gcgagttccg
     2521 ctaccaggag acgctgttcg accagatggc gcgccagtac gagctcgcgc gcatcgacga
     2581 ggccaaggaa ggcgcgctga tccaggtcgt cgatccggcg cggccggccg agcgcaagag
     2641 cggcccgcgc cgcctgcgca tcgccatcga ggcgggtgtc gccgcgctgc tgctgttcgc
     2701 gctcggactg gtggtgcgcg accgctggcg catggcggcc ggcgatcccc atgtggcgca
     2761 gcgcctgcgc gtgctgcgcg actccctgcg ccggcactga gcggccgctc gcggcccgca
     2821 ccggcagccg ggccgatgcg cgcaccgcgg cgcgccggga gccgccgccg gcggttccgt
     2881 cggcgcggac gtggcgcaac gcacaaaagg gcgcgaatta taggcagccc acctgtcgat
     2941 ctgccttcga atacatgtgc ggatgcccgc cggcggccgc gccactggta aactgtggcc
     3001 cctcggaact gctgaccgcc gcctcctgct gcacgtgccc cacccggtgc gctcgcacct
     3061 cgtggccgcc gtcgcggcgc cctcctgcgg ccagccaggt cgcgccaggc cagtccgccg
     3121 gtgcaacccc gcgccggcgc tcgcctccag cccagaccca agaatgaaga agatcgtttc
     3181 cgtcgtcggc gcccgccccc agttcgtcaa ggcagccgtg gtcagcgagc aactgcggcg
     3241 cgtcggcgtc caggaggtca tgatccacac cggccagcac tacgacgcca acatgtccga
     3301 cgcgttcttc gacgcgctgg gcatccccgc gccgcgctac aacctgggca tcggcggcgg
     3361 cgcccacggc gagatgaccg gccggcagct gatcgagatc gagcgccgcc tgatggacga
     3421 gaagccgcag gccgtgctgg tctacggcga caccaacagc acgctggccg gcgcgctggc
     3481 cgccgtcaag ctgcacatcc ccatcgccca cgtcgaggcc ggcctgcgct cgttcaacac
     3541 gcgcatgccc gaggagatca accgcatcgt gaccgatcgc atctccgcgg tgctgttcac
     3601 gccgtcgcgc ctggcgtccg acaacctgcg cgccgagggc gtggccgaca gccggatcgt
     3661 cgaggtcggc gacgtcatgt acgacgccgc gctgcgctac ggcaacgcgc cggcggccgg
     3721 cggcaacggc cgcccgttct tcctcgtcac catccacc
//
LOCUS       DATCRO010000273         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_992966, whole genome shotgun sequence.
ACCESSION   DATCRO010000273 DATCRO010000000
VERSION     DATCRO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992966"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VIN75_07465"
     CDS             <1..421
                     /locus_tag="VIN75_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974039.1"
                     /translation="RGFDAMRVVLHQAEADLHHRFALAPPDGMTLPTAALHHGEAALA
                     HGQRLTGWSPRATLLDFLAERWADTGDHERAYGYARRALAAKERETAQKMSYPLALLR
                     VRRHAEVRAGPPPALPAAAPAAARTLLPANRRAPRIL"
     gene            444..2876
                     /locus_tag="VIN75_07470"
     CDS             444..2876
                     /locus_tag="VIN75_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY8974040.1"
                     /translation="MNSAPFSMPRCPQPLRPLALGVGLALLTMCAGAATRLQAPDARR
                     VAQAVDAAHERSGLELAARTSLVNQFGQTVVRASQTWQGHRVWGSEVVVRSDKTGKST
                     IAATSASAGVAPAGSPTLSQDQAIAVARRAIGLQGRSAPPRAELVVFPTRCTGDVALA
                     WNESTHRYGFDRANSVMAVRPADPFVWAYEVHLFANNRLDGVRDMTYVVDARTGAILH
                     VRNGRQSIAPMNPPVQLPTDVAAVGTGYSQYSGTVKLDTTMHADGTFQMIDRTRGSRW
                     NPYLHDNFYDAQGNQVLAPDGNPISVVGLQSITETHGGFGDSWVEDNWWFDGNPTDTW
                     GDGRQFVMFPYGGETTANGQTAAVDAHHGMALTWDFYLNVFGRNGIDNIGTSPISVVH
                     IVNDHGYYYDNAYWTDYVFGMFYSDGTKNPGVDPSTGLPTAGDPNGLDTLTSIDIIGH
                     EMTHGVTANSDGLVYEGESGGLNESTSDFMGSMVEAWATRTGPDWAIPNTGTDWLMGA
                     QISDAPLRSMIHPSIDGVSSDIWYAGIQYLDVHYSSGPLNRFFYFLSQGASPDPDSDS
                     YSPYLPGGMKGIGNDHAAHIWYTAMTEWLMPLARYADARNAAINAAEEDYGPGTPEVD
                     AVKKAFAAINVGSADDTPRVRIDFPVAHPGGTALNNSGNGVFTRMPIVAMGTTVHLAA
                     EVHNTNDTSVTWKTGGSSAAYNSRGFRNAGGTVTPDGGWSPDSVWGFHAMTVTSNADP
                     LQFAEGVAWVIDADADGDTEFDAIDLGGVALSWGLDGWVEASHSMVGDGFDDSMDVTA
                     IIEAFRNAYGGT"
     gene            complement(2895..4637)
                     /locus_tag="VIN75_07475"
     CDS             complement(2895..4637)
                     /locus_tag="VIN75_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEY8974041.1"
                     /translation="MQLFAAEEEVSALEASLSELRGDERLASTVALAWHLRQRDSTRA
                     VRLVNGVMAPLGGASPQGSTPVPHRARAALAVCEAKSLLCEFDAAERWLAEARAHLDP
                     VSDAQAEGDAFLVESLLAKTRGQRERELSAFEQAIAFYAASGAPERLAIARMWASYER
                     SFTQPELESTLGLEPPGKAWPAACVAWNALWSAARALGLSFRSPSTSAAVFQHAAEQA
                     RQVGMVRLEIVAMINAGAARQGTGDMDMASDCFDLAATRARMTGWPTLVGAANTRLGE
                     LLQTLGALDESRALLSEAILQLSVVPRSMHVAIACAALAQTLLAMERPVEALEPMGEA
                     IAMYRETGFPHNLALNLIFQARVLAAAGRVDEALAVLAEAQEMIARLELDSLRVGVSD
                     VLADLHHRFDLPPPVGMVLPSGALHFAEATLAQGLAIEGWKAPARLYAFLAERWAEAG
                     DHARAYGHARQALQAKEQESTQKMNYPLALARLRRRTTRASDAAAEPSVAPLAGADRE
                     RRPGGKSLTPKERSILLLLARNYSNKEIAKAIAMSDETVKWHLKNLFNKLGAGSRKHA
                     VTRARTLGFISFSA"
     gene            complement(4709..>5783)
                     /locus_tag="VIN75_07480"
     CDS             complement(4709..>5783)
                     /locus_tag="VIN75_07480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8974042.1"
                     /translation="VRCFRHPGVTAPAAAGRAAVLADAWTPEFARWLQAHAADADWFT
                     PWQRQARRAAGAWSPDGTGRHVVARWSSWRRTWLLRRRAGEGGGRQEALQRANGWLAR
                     DFARRLQSRHTALVVDQGLLVPLADAGVLQGRHYEVLVHALPAGELQRRLDVAARAWP
                     QAPSLRDFRADVGFCDSELRALRGARRLVTPHADVARHLRGLGLAQVECLAWIAPPAD
                     ATPAPRPAPASTPLVAFPASALARKGAHELAQALRQLGWRLLVLGTPSPDASLWRGID
                     AEHANYRDAAWIARADVVALPAHVEHAPRALLRALAHGLPVVATAACGLPAAPGWHEV
                     AAGDVAGLVDALQAALAGARTRY"
BASE COUNT          829 a         2176 c         2054 g          724 t
ORIGIN      
        1 gcgcggcttc gacgcgatgc gcgtcgtgct gcaccaggcc gaggccgacc tgcatcaccg
       61 tttcgcgctc gcgccgcccg acggcatgac gctgccgacc gccgcgctgc atcacggcga
      121 agccgcgctc gcgcacggcc agcgcctgac cggctggagc ccgcgcgcga cgctgctgga
      181 cttcctggcc gagcgctggg ccgacaccgg cgaccacgag cgcgcctacg gctacgcgcg
      241 ccgcgcgctc gccgccaagg agcgcgagac ggcccagaag atgagctatc ccctggcgct
      301 gctgcgcgtg cgccgccacg ccgaggtgcg tgccggcccg ccgccggccc tgcccgcggc
      361 cgcgcccgcc gcggcgcgca ccctgcttcc cgcgaaccgc cgcgcgccgc gcatcctgtg
      421 acatccaacc cgagcccgca tccatgaact ccgctccctt ttccatgccg cgctgccccc
      481 agcccctgcg cccgctcgcg ctgggcgtcg gcctcgcgct gctgacgatg tgcgccggcg
      541 ccgcgacgcg cctgcaggcg ccggacgccc gccgcgtggc gcaggccgtc gatgccgcgc
      601 acgagcgctc gggcctggag ctggcggcgc gcacctcgct cgtcaaccag ttcggccaga
      661 cggtcgtgcg cgccagccag acctggcagg gccatcgcgt ctggggttcg gaggtggtgg
      721 tgcgctcgga caagaccggc aagtcgacca tcgccgcgac gtccgcatcg gccggcgtcg
      781 cgccggccgg cagcccgacg ctgagccagg accaggccat cgccgtcgcg cggcgcgcga
      841 tcggcctgca gggacgcagc gcgccgccca gggccgagct cgtcgtcttc ccgacgcgct
      901 gcaccggcga cgtcgccctc gcctggaacg agtccacgca ccgctatggc ttcgaccgtg
      961 cgaacagcgt gatggccgtg cgtccggccg acccgttcgt ctgggcgtac gaggtgcacc
     1021 tgttcgcgaa caaccgcctc gacggcgtgc gcgacatgac ctacgtcgtc gacgcgcgca
     1081 ccggcgcgat cctgcatgtg cgcaacggac gccagtccat cgccccgatg aacccgcccg
     1141 tgcagctgcc caccgacgtg gccgccgtgg gcaccggcta cagccagtac agcggcaccg
     1201 tcaagctcga cacgacgatg cacgccgacg gcacgttcca gatgatcgac cgcacccgcg
     1261 gctcgcgctg gaacccgtac ctgcacgaca acttctacga cgcccagggc aaccaggtgc
     1321 tggcgcccga cggcaacccg atctcggtcg tcggcctgca gtcgatcacc gagacgcatg
     1381 ggggcttcgg cgacagctgg gtcgaggaca actggtggtt cgacggcaac ccgaccgaca
     1441 cctggggcga cggacgccag ttcgtgatgt tcccgtacgg cggcgagacg accgccaacg
     1501 gccagaccgc ggcggtcgat gcgcaccacg gcatggcgct gacctgggac ttctacctga
     1561 acgtcttcgg ccgcaacggc atcgacaaca tcggcacctc gccgatctcg gtggtgcaca
     1621 tcgtcaacga ccacggctac tactacgaca acgcgtactg gaccgactat gtcttcggca
     1681 tgttctattc ggacggcacg aagaaccccg gcgtcgaccc gagcacgggc ctgcccacgg
     1741 cgggcgaccc gaacggcttg gacacgctca cctcgatcga catcatcggc cacgagatga
     1801 cgcacggcgt cacggccaac tccgacggcc tcgtctacga aggcgagtcg ggtggcctga
     1861 acgagtcgac gtccgacttc atgggctcga tggtcgaggc ctgggccacg cgcacgggcc
     1921 cggactgggc gatccccaac accggcaccg actggctgat gggcgcgcag atctcggacg
     1981 ccccgctgcg ctcgatgatc cacccgagca tcgacggcgt cagctccgac atctggtacg
     2041 ccggcatcca gtacctggac gtgcactaca gcagcggccc gctgaaccgc ttcttctact
     2101 tcctgtcgca gggcgcgtcg ccggatccgg actcggactc gtacagcccg tacctgcccg
     2161 gcggcatgaa gggcatcggc aacgaccatg cggcgcacat ctggtacacg gcgatgaccg
     2221 agtggctcat gccgctggcc aggtacgccg acgcgcgcaa cgcggccatc aacgcggccg
     2281 aggaggacta cggccccggc acgcccgagg tcgatgccgt caagaaggcg ttcgcggcca
     2341 tcaacgtcgg cagcgccgac gacacgccgc gcgtgcgcat cgacttcccc gtcgcgcatc
     2401 cgggcggcac cgcgctgaac aattccggca acggcgtctt cacgcgcatg cccatcgtgg
     2461 ccatgggaac gaccgtgcac ctggccgccg aggtgcacaa cacgaacgac accagcgtca
     2521 cgtggaagac cggcggcagc agcgccgcct acaacagccg cggcttccgc aacgccggcg
     2581 gcaccgtcac ccccgacggc ggctggtcgc ccgacagcgt ctggggtttc catgcgatga
     2641 ccgtcaccag caacgccgac ccgctgcagt tcgccgaagg cgtcgcgtgg gtgatcgacg
     2701 ccgacgccga cggcgacacg gagttcgacg cgatcgatct cggcggcgtc gcgctgtcct
     2761 ggggcctgga cggctgggtc gaggcgagcc acagcatggt gggcgacggc ttcgacgaca
     2821 gcatggacgt gacggccatc atcgaggcgt tccgcaacgc gtacggaggg acatgactcc
     2881 gtgcggccgc ccgctcaggc gctgaagctg atgaacccca gcgtgcgcgc ccgcgtgacg
     2941 gcgtgcttgc gcgagccggc gcccagcttg ttgaacaggt tcttcagatg ccacttgacc
     3001 gtctcgtcgc tcatcgcgat ggccttggcg atctccttgt tggagtagtt gcgcgcgagc
     3061 agcagcagga tgctgcgctc cttgggcgtc agcgacttgc cgcctggccg gcgctcgcgg
     3121 tcggcgcccg ccagcggcgc caccgacggc tcggccgcgg cgtcgctcgc ccgcgtcgtg
     3181 cgccggcgca gccgcgcgag cgccagcggg tagttcatct tctgggtcga ctcctgctcc
     3241 ttggcctgca gcgcctggcg cgcgtggccg tacgcgcggg catggtcgcc cgcctcggcc
     3301 cagcgctccg cgaggaacgc gtacagccgc gccggcgcct tccagccctc gatggccagg
     3361 ccctgggcca gggtggcctc ggcgaagtgg agagcaccgc tgggcaggac catgccgacg
     3421 ggcgggggta ggtcgaagcg gtgatgcagg tcggcgagca cgtcgctgac gcccacgcgc
     3481 agcgagtcga gctccaggcg cgcgatcatc tcctgcgcct cggccagcac ggcgagggcc
     3541 tcgtccacgc ggcccgcggc ggcgagcacg cgcgcctgga agatcaggtt cagcgcgagg
     3601 ttgtggggaa agcccgtctc gcggtacatg gcgatcgcct cgcccatcgg ctcgagcgct
     3661 tcgacgggac gctccatcgc gagcagcgtc tgcgccagcg cggcgcacgc gatcgccacg
     3721 tgcatgctgc gtggcaccac cgacagctgc aggatggcct cgctgagcag cgcgcggctt
     3781 tcatcgagcg cgcccagcgt ctgcagcagc tcgcccaggc gcgtgttggc cgcgccgacc
     3841 agcgtgggcc agccggtcat gcgcgcccgc gtggcggcga ggtcgaagca gtcgctggcc
     3901 atgtccatgt cgccggtgcc ctggcgcgcg gcgccggcgt tgatcatggc cacgatctcc
     3961 aggcgcacca tgccgacctg gcgcgcctgt tcggcggcgt gctggaagac ggcggccgag
     4021 gtcgacggac tgcggaacga caggcccagc gcgcgcgcgg cgctccacag cgcgttccat
     4081 gcgacgcagg ccgcgggcca ggccttgccg ggcggctcca ggcccaacgt ggattcgagc
     4141 tcgggctgcg tgaacgagcg ctcgtagctc gcccacatgc gagcgatcgc gagccgctcc
     4201 ggcgcgccgc tggcggcgta gaacgcgatc gcctgctcga aggccgaaag ctcgcgttcg
     4261 cgctggccgc gcgtcttggc gagcaggctc tcgacgagga aggcgtcgcc ctcggcctgc
     4321 gcgtcggaga cgggatccag gtgcgcgcgc gcctcggcca accagcgttc ggcggcgtcg
     4381 aactcgcaca gcagcgactt cgcctcgcat acggccagcg cggcgcgggc gcggtgcggc
     4441 acgggcgtcg acccctgggg cgatgcgccg ccgagcggcg ccatcacgcc gttgaccagt
     4501 cgcacggcgc gcgtgctgtc acgctggcgc aggtgccagg cgagggcgac ggtggacgcc
     4561 aggcgctcgt cgccgcgcag ctcggacagc gaggcctcga gcgcggagac ttcctcttcg
     4621 gcggcgaaca actgcatggg gcaattgtag ggatcgcgcc gcgcgcgagc caccgtcgga
     4681 acccgggccc gcccggagcg gcgtggcttc agtaccgggt gcgggcgccg gccagcgccg
     4741 cctgcaacgc gtccacgaga cccgccacgt cgccggccgc gacctcgtgc cagcccggcg
     4801 ccgcgggcag gccgcaggcc gccgtcgcca ccaccggcag cccgtgcgcg agcgcccgca
     4861 gcaacgcgcg tggcgcgtgc tcgacgtgcg ccggcagcgc gacgacgtcg gcccgcgcta
     4921 tccacgccgc atcgcgatag ttggcgtgtt cggcatcgat gccgcgccac aggcttgcat
     4981 cgggcgacgg cgtgcccagc accagcagcc gccaacccag ttgccgcagc gcctgggcga
     5041 gttcgtgcgc gcccttgcgg gccagcgcgc tcgcggggaa ggcgaccagc ggggtcgatg
     5101 ccggcgcagg acgcggcgcg ggcgtcgcat cggcgggcgg cgcgatccag gccaggcact
     5161 cgacctgcgc gaggcccagg ccgcgcaggt ggcgcgcgac gtccgcgtgc ggcgtgacga
     5221 gccggcgcgc gccgcgcagc gcgcgcagct cgctgtcgca gaagccgacg tcggcgcgga
     5281 agtcacgcag ggacggcgcc tgcggccagg cgcgcgccgc gacgtcgagc cggcgctgca
     5341 gctcgccggc aggcagcgcg tgcacgagca cctcgtagtg gcgaccctgc agcacgcccg
     5401 cgtcggccag cggcaccagc aggccctggt cgacgaccag cgccgtgtgc cgcgattgca
     5461 gccgacgcgc gaagtcgcgc gcgagccacc cgttcgcgcg ctggagcgct tcctggcggc
     5521 cgccgccctc gccggcgcgg cgccgcagga gccacgtgcg gcgccacgac gaccagcgcg
     5581 cgacgacgtg gcgtcccgtg ccgtcgggcg accatgcgcc ggcggcgcgg cgggcctggc
     5641 gttgccacgg cgtgaaccag tcggcgtcgg cggcgtgcgc ctgcagccag cgcgcgaact
     5701 ccggcgtcca ggcgtcggcc agcacggcgg cgcggccggc cgcggccggc gccgtcacgc
     5761 cgggatggcg gaagcagcgc acc
//
LOCUS       DATCRO010000274         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_531763, whole genome shotgun sequence.
ACCESSION   DATCRO010000274 DATCRO010000000
VERSION     DATCRO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531763"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..862)
                     /locus_tag="VIN75_07485"
     CDS             complement(176..862)
                     /locus_tag="VIN75_07485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8974043.1"
                     /translation="MASSTITLTAADGFQFPAYVSEPASKARGSIVVLQEIFGVNSHI
                     RSVADRFAAAGYLAIAPATFERAERGVEMGYGPDDMKRGSALKAAIEALPAPGVLQDI
                     QAAVERAGESGRVGVIGYCWGGLLSWRAAAQVQGLAAAVTYYGGGMTMGKEPDRKPAV
                     PTLSHFGELDKHITLDSVKAFEQKHPEVEVHLYAADHGFNCDQRGSYNAGAAATALER
                     TLFHFARHLG"
     gene            1452..3137
                     /locus_tag="VIN75_07490"
     CDS             1452..3137
                     /locus_tag="VIN75_07490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY8974044.1"
                     /translation="MIRSGIAGNPLATTLALAAALALAGAAHAEGAHTKHVHAAIQGG
                     EAQMVGSPAPTQVMNLDIVLPVRDREGLKALAQAVSDPDSPMFHHYLTPAQFTERFGP
                     TQADYDTVVRYLEDRGLKVTGGSRDGMEVQVRGTVDKVEGAFHVTMRNYRHPTENRTF
                     FSPDREPTTDMAVKLWHVSGMDNYSIPHPKYVKRSDYAAAKGISVDAVVSHAATGSGP
                     SGSFTGSDMRAAYYGTGSLTGAGQHVALFEYYGTDLADLTTYYKNIGQTNNVPVTLIS
                     TDGTSTSCLAKNNCDDGEQELDITQALGMAPGLAGLEMYVGSTDTAIISAMTTHSPLA
                     LAIGCSWGWTPADASTLDPYFEKMAVQGQSFFVASGDNSTWKKSGAAEAWPGDDAHII
                     SVGGTDLVTASAGGAWASETAWVDSGGGISPDRVAIPSWQQISGVITTANKGSTTLRN
                     GPDVAANANFTFYTCDNQTTCQANTYGGTSFAAPMWAGLVALANQQAASTGKSPVGFF
                     NPKIYAQNVTSSYSTGFHDVTSGTSGSYKAGTNFDLVTGWGSPKPAIIDQLLK"
     gene            3299..>3647
                     /locus_tag="VIN75_07495"
     CDS             3299..>3647
                     /locus_tag="VIN75_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974045.1"
                     /translation="MKNILAASLLLALVGCGDSGPDRGDPPGPTTFNIGGTITGLSGS
                     GLVLANGSESVSVTAGATQFTLPAHASGTAYSVAVQTQPGGLTCTVSAGSGTIGGQPV
                     TDVKVDCHGHTLYV"
BASE COUNT          626 a         1312 c         1205 g          504 t
ORIGIN      
        1 ggcccgcggc aaggccaaga tgagtgccct cggcgccgcc atgcataagc tcgcgcaatt
       61 gtgcttcggc gtcgttcaca caggccagcc ctaccgcgct gattggcctg tcaaggcttg
      121 acgcccgaga cggtatctac ggatgcacgg aacgtacatc agtcgcgcta agtcgtcagc
      181 ccaggtgcct ggcgaaatgg aacagcgtcc gctccagcgc cgtcgccgcc gcgccggcgt
      241 tgtacgaacc gcgctggtcg cagttgaagc cgtggtcggc ggcgtacagg tgcacctcga
      301 cctcggggtg cttctgctcg aaggccttca cgctgtcgag cgtgatgtgc ttgtccagct
      361 cgccgaagtg cgacagggtc ggcacggcgg gcttgcggtc cggctccttg cccatcgtca
      421 tgccgccgcc gtagtacgtc acggccgcgg ccaggccctg gacctgcgcg gccgcgcgcc
      481 atgacagcag cccgccccag caatagccga tgacgcccac gcggcccgac tcgccggcgc
      541 gttcgacggc cgcctggatg tcctgcagca cgcccggcgc gggcagcgcc tcgatggcgg
      601 ccttgagcgc gctgccgcgc ttcatgtcgt cggggccgta gcccatctcc acgccgcgct
      661 cggcgcgctc gaacgtggcc ggcgcgatcg cgaggtagcc cgcggccgcg aagcggtcgg
      721 ccacgctccg gatgtgcgag ttcacgccga agatctcctg cagcaccacg atggacccgc
      781 gcgccttgga agcaggctcg ctgacgtagg ccgggaactg gaagccgtcg gcggcggtga
      841 gggtgatcgt ggaagacgcc atggaggact cctgggatgc gggatcgaag agggcttgcc
      901 cgtttgtaca tcgcgtcgcg agaccccgtc gtggcgaggc cggcggcccg acgaagcggc
      961 aagtcgcgcg ccccggcggc gggcccgcga agtcggccgc gccgcccatc cgggaatatc
     1021 agatattcag gctccgcaca gtttgtcgac agtttggcga cccctacccg gattcgggga
     1081 ttgcgccagc cggcgatgaa taaaccagtc accgcgcatt tgaatgcacc aagcccggga
     1141 ttatggaatc gccgggatcc accgccggca cggggcgccg gcctcgacag gccgctccct
     1201 cggccgtgcc caggcgtgac gcgcacctcc acgacgcatc tcgaaggcac cgaaggtgcg
     1261 cgcgatcagc gcaggggctt gcttcggcca tggcctccaa tacctcgcaa gttggcatcc
     1321 aagcaacatc cacatactcg cgggaaaacg cgatgagcgt ttccgatatc tctcgacgac
     1381 acgcctcaaa acctatattg cggcctccgg gcacaacccg gctccttcac ttgcacatat
     1441 tggagttgcc aatgatccga tcgggaatcg caggaaatcc gctggccacc acgctggcac
     1501 tggccgccgc gctggcactg gccggcgccg cacacgccga gggcgcgcac acgaaacacg
     1561 tccacgcggc catccagggc ggcgaggcgc agatggtggg cagccccgcc ccgacgcagg
     1621 tgatgaacct ggacatcgtg ctgcccgtgc gcgaccgcga aggcctgaag gcgctggcgc
     1681 aggcggtgtc cgacccggac agcccgatgt tccaccacta cctgacgccg gcgcagttca
     1741 ccgagcgctt cggcccgacg caggccgact acgacacggt ggtgcgctac ctggaggacc
     1801 gcggcctgaa ggtgaccggc ggctcgcgcg acggcatgga agtgcaggtg cgcggcacgg
     1861 tcgacaaggt cgagggcgcg ttccacgtga cgatgcgcaa ctacaggcac ccgaccgaga
     1921 accgcacgtt cttctcgccg gaccgcgagc ccacgaccga catggccgtc aagctgtggc
     1981 acgtctcggg catggacaac tactccatcc cgcatcccaa atacgtcaag cgcagcgact
     2041 acgccgccgc caagggcatc tcggtcgacg ccgtcgtcag ccacgccgcc accggctcgg
     2101 gcccgtcggg ctcgttcacc ggcagcgaca tgcgcgccgc ctactacggc accggctcgc
     2161 tcaccggcgc cggccagcac gtcgcgctgt tcgagtacta cggcaccgac ctggccgacc
     2221 tgaccacgta ctacaagaac atcggccaga ccaacaacgt gccggtgacg ctgatctcga
     2281 cggacggcac cagcacctcg tgcctggcca agaacaactg cgacgacggc gagcaggagc
     2341 tcgacatcac gcaggcgctc ggcatggcgc cgggcctggc cgggctcgag atgtacgtgg
     2401 gctcgaccga cacggccatc atcagcgcga tgaccacgca cagcccgctc gcgctggcga
     2461 tcggctgctc gtggggctgg acgcccgccg acgcgagcac gctcgacccg tacttcgaga
     2521 agatggccgt gcagggccag agcttcttcg tggcctcggg cgacaactcg acgtggaaga
     2581 agagcggcgc ggccgaggcc tggccgggcg acgacgcgca catcatctcg gtgggcggca
     2641 ccgatctcgt gaccgccagc gccggcggcg cctgggcgtc ggaaaccgcc tgggtcgact
     2701 ccggcggcgg catctcgccg gaccgcgtcg cgatcccgag ctggcagcag atctcgggcg
     2761 tgatcaccac cgccaacaag ggctcgacga cgctgcgcaa cggcccggac gtcgcggcca
     2821 acgcgaactt cacgttctac acctgcgaca accagaccac ctgccaggcc aacacctacg
     2881 gcggcaccag cttcgccgcg ccgatgtggg cgggcctggt cgcgctcgcc aaccagcagg
     2941 ccgcgagcac gggcaagtcg ccggtgggct tcttcaaccc gaagatctac gcgcagaacg
     3001 tgacgtccag ctattcgacc ggcttccatg acgtgaccag cggcacgtcc ggcagctaca
     3061 aggccggcac gaacttcgac ctcgtcacgg gctggggcag cccgaagccg gcgatcatcg
     3121 accagttgct gaagtaaccg gcattcctct cggtccggcc gcgggacgaa gggcgcgcct
     3181 tcgtcccgcc gccttttcgg acgcctccgg cagtctggta cgctgcggcc gcgccgcgcg
     3241 acggtggaca cccgtcccgc gcgcgacgac gacaacgatc cagaccaggg aggaatccat
     3301 gaagaacatc cttgcggcga gcctgctgct cgccctcgtc ggctgcggcg attccggccc
     3361 ggaccgcggc gatccgccgg gcccgaccac gttcaacatc ggcggcacca tcaccggcct
     3421 gagcggctcc ggcctcgtgc tggccaacgg cagcgagagc gtcagcgtca cggcgggggc
     3481 cacgcagttc acgctgccgg cgcacgccag cgggacggcc tattccgtgg cggtgcagac
     3541 gcagcccggc ggcctgacct gcacggtgag cgcgggctcg ggcacgatcg gcgggcagcc
     3601 cgtcaccgac gtcaaggtcg attgccacgg ccacacgctc tacgtgg
//
LOCUS       DATCRO010000275         6175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_745840, whole genome shotgun sequence.
ACCESSION   DATCRO010000275 DATCRO010000000
VERSION     DATCRO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745840"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1222)
                     /locus_tag="VIN75_07500"
     CDS             complement(293..1222)
                     /locus_tag="VIN75_07500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8974046.1"
                     /translation="MQQRSHARTVRSIGVAATSALLLAALSACGGGGDSSPATVSAMN
                     VATTKYGVPGLITVTGTHLGNLSISAPGCRNVARLSSGATVSSDTTSYYSCTPSGTYS
                     STITASTQGTTLASQTLTVQPPVVTMSVSGGGVIGNIVFNLKGNVAPVTVDNFLDYVN
                     AGFYTGLVFHRVAHFNNPLVGPEYFVVQGGNYGAVTSGTLPAAVKTPLYNPIKFEGSG
                     GNNVQWTAAMARGQDRDSATSGFFFNVINNSAAFETSSGPGYAVFADVSGSAAVITQI
                     TQAACTSLAPSNFNDGSCVPIPNVVITTATQTQ"
     gene            complement(1337..3235)
                     /locus_tag="VIN75_07505"
     CDS             complement(1337..3235)
                     /locus_tag="VIN75_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974047.1"
                     /translation="MSQSHPSQFALLRQRRFAPFFWTQFFGAGNDNVLKFAFTVLVTY
                     QLNVSWLPPEKAGLVISAVFILPFVLLSATSGQLADKYDKARVMRFIKSFEILIMVLA
                     IWGFVGQLTWLLLGCLFLMGVHSTLFGPVKYAYLPQHLDDHEITGGNGMVEMGTFVAI
                     LLGQLAGGLLVVVPGIGPELAGGACLAIAVIGRLTAQAIPSSPAHDTALKINWNPVTE
                     TLRNLKLARTDIVVFRSLLGISWMWFFGATFLSQFPSFAKDVLHGDVHVASLLLVLFS
                     VGVGVGSLLCEVLSRRQVEIGLVPLGAIGMSVFAIDLYFAVSHLAAPTTTFWTLSSFL
                     AQGQHVRPMIDLFMLSLSAGLYSVPMYALIQTRALASHRARIIASNNILNALFMIASS
                     LIAGALLHFGVSIGGVFLITGIANAVVAGYIFCLVPEYLLRFIAFVLTRTVYRFRMQG
                     DTNIPTTGAAIVVCNHVSFVDPVLLMAASPRPIRFLMDARIFRTPGLGLFFRLAKAIP
                     IAPKNEDPAAYEAAFAAADQVLAEGDLLGIFPEGGITNDGTLKPFKGGVMRILEQRPV
                     PVIPLALQNLWGSFFSRVEGKAMSKPFRRGLFSRVGLNVGPVIAADAVTPEALHQQVG
                     QLLATKVV"
     gene            complement(3348..4229)
                     /locus_tag="VIN75_07510"
     CDS             complement(3348..4229)
                     /locus_tag="VIN75_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEY8974048.1"
                     /translation="MSRLSRWGRVLAAAAGLAVATLAGAGQTCDTRPPDTYAMTRALG
                     LAERVQQSLDASGARVVIIARAGQDLTKYGLQWSHLAFAYRDDPAPAALPVAVPTSPV
                     LGQISDQPVAVPLQRRGTWRIVHKLNQCGTADAAVFRQGLAEFFLDTPFRYEAAYVVP
                     DPEVQAALLRTVVDDQRLVQWHTPTYSVVAYPWSTRYQQSNQWALETLAGSLDPAATT
                     RERAQAWLQLHDYQPTVLHIDALTRLGARMTRANVAFDDHPNSKRFSDHIETVTVESM
                     FAWMQRMQIAGRPTVVR"
     gene            complement(4226..4594)
                     /locus_tag="VIN75_07515"
     CDS             complement(4226..4594)
                     /locus_tag="VIN75_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974049.1"
                     /translation="MAAGTTQAQGEPSRISEESLVPVAISVALPVVLVAGAGSIVVTG
                     VEASADGVVWFVENVADGVKGSIRFAANAVSASAMVAGTVVVVTVVATGMLLSAAGHV
                     IAFIPNEIGRSLSYNQRVSR"
     gene            4780..5529
                     /locus_tag="VIN75_07520"
     CDS             4780..5529
                     /locus_tag="VIN75_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8974050.1"
                     /translation="MSTTAEIVNVLKSELKLAKLTYADLGRELGLAESSVKRMFAKGD
                     MPLKRIDEICRVLRCDFSELARKVADLELLRDELTLDQERAVVADKRLLVMALCTLSQ
                     WPFEQIMGMYAFTEAEAVRHLTQLDRLGIIELKPLNRYRLKVAKTFRWRAHGPVMQFF
                     RDHGVADYFSGGFNGECEMVSLVHGHLSLPMAKVFQERLQRVAQDFAQQHLADQRLPA
                     AHKRGFTAVMALRSWTFGPLRELLRDPNIEP"
     gene            complement(5554..>6175)
                     /locus_tag="VIN75_07525"
     CDS             complement(5554..>6175)
                     /locus_tag="VIN75_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8974051.1"
                     /translation="PMTVYDVLLESATITEAAQKSGSGGYDVLGANRELAGAEVELVA
                     LERRNERLRRALKAVDGDYDFVLIDCPPSLNLLTLNGLCSAHGVIVPMQCEYFALEGV
                     SDLVNTIKQVHANLNRDLELIGLLRVMYDPRITLQAHVSEQLKAHFGEKVFNTVIPRN
                     VRLAEAPSYGQPGVVFDPTSRGAVAFVEFAQEMVAREKGPALADVR"
BASE COUNT         1012 a         1966 c         2246 g          951 t
ORIGIN      
        1 ggcgatgagc cgccgccacc gccgcaggcg gacaggccgg cgacggtggc catggtgagg
       61 gcgaggagcg cgaggcgatc cgcgcgttgg gtggaagtgg cgctcatcgt gggacgggaa
      121 agtcggatga aggtacggaa cgcgcgcgtg gcgcgcgccg tcgcgtttcg gcgtggtatt
      181 ggcgccgggc ggggcgtgga catgggctcg tgcggctcgc cgtcctcaac cggcgcgacg
      241 ggacgcgact cgtacgggca cgtcgcgcga cgtgcccggc cggcgcgaag tcctactgcg
      301 tctgcgtggc cgtcgtgatc acgacgttgg ggatcggcac gcacgagccg tcgttgaagt
      361 tcgacggcgc caacgacgtg caggcggctt gcgtgatctg cgtgatcacg gcggcactgc
      421 cgctgacgtc ggcgaacacg gcatagcccg ggccgctcga cgtctcgaag gcggccgagt
      481 tgttgatcac gttgaagaag aagccggagg tggcggagtc gcggtcctgg ccgcgggcca
      541 tcgccgctgt ccactgcacg ttgttgccgc cgctgccttc gaacttgatc gggttgtaca
      601 gcggcgtctt gaccgcggcg ggcaaggtgc cgctggtcac ggcgccgtag ttgccgccct
      661 gcaccacgaa gtactcggga ccgaccagcg ggttgttgaa gtgggcgacg cggtggaaca
      721 cgaggccggt gtagaagccc gcgttcacgt agtccaggaa gttgtcgacc gtgacgggcg
      781 cgacgttgcc cttcaggttg aacacgatgt tgccgatcac gccgccgccg ctgactgaca
      841 tcgtcacgac gggcggctgc acggtgaggg tctgcgaggc cagcgtggtg ccctgcgtgc
      901 tggccgtgat cgtgctggag taggtgccgg acggcgtgca cgagtagtag ctggtggtgt
      961 ccgagctgac cgtggcgccg ctggacagtc gcgcgacgtt tctgcagccc ggggcgctga
     1021 tgctgaggtt gcccaggtgc gtgccggtga cggtgatcag gcccggaacg ccgtacttgg
     1081 tggtcgccac gttcatcgcg ctgacggtcg ccggcgagga gtcgccgcct ccgccgcagg
     1141 cgctcagcgc cgcgagcagg agggctgacg ttgccgcgac gccgatcgag cgcacggtgc
     1201 gagcatggga gcgttgctgc atgatggatg gggaagacga aagcgtgaat ggtagggcat
     1261 gcacgcgcga cattcggtta agtccgcgtt aacggggccg ggcgtggcgc gcaacgtggg
     1321 gtatgcgacc gacggctcag acgaccttcg tggcgagcag ttgtcctact tgctggtgca
     1381 aggcctcggg cgtcaccgcg tccgcggcga tgaccggacc cacgttgagc ccgacgcgac
     1441 tgaacagccc acggcggaac ggcttgctca tggccttgcc ctccacgcgc gagaagaacg
     1501 agccccacag gttctgcagg gccagcggga tcaccggcac gggccgctgc tcgaggatgc
     1561 gcatgacgcc gcccttgaac ggcttgagcg tgccgtcgtt ggtgatgccg ccttcgggga
     1621 agatgcccag caggtcgccc tcggccagca cctggtcggc ggcggcgaac gccgcttcgt
     1681 aggctgccgg gtcctcgttc ttcggggcga tgggaatcgc cttggccaga cggaagaaca
     1741 ggccgagccc gggcgtgcgg aagatgcggg cgtccatcag gaatcggatc ggccgcgggc
     1801 tggcggccat cagcagcacg gggtcgacga agctgacgtg gttgcacacg acgatggcgg
     1861 cgccggtggt cgggatgttc gtgtcgccct gcatgcggaa gcggtagacc gtgcgcgtca
     1921 ggacgaaggc gatgaaacgc agcaggtatt ccggcacgag gcagaagatg tagccggcca
     1981 cgacggcgtt ggcgatgccg gtgatcagga acacgccccc gatgctgacg ccgaagtgca
     2041 gcagcgcgcc ggcgatcagc gaactggcga tcatgaacag cgcattcagg atgttgttcg
     2101 acgcaatgat gcgggctcgg tggctagcaa gcgctcgcgt ctggatcagc gcatacatcg
     2161 gcacgctgta caacccggcg ctgagcgaca gcatgaacag gtcgatcatc gggcgcacgt
     2221 gctggccctg tgccaggaag ctcgacaggg tccagaacgt cgtggtgggc gcggccaggt
     2281 ggctgacggc gaagtacagg tcgatcgcga acacgctcat cccgatggcg cccagcggca
     2341 cgaggccgat ctcgacctgg cgccgggaca gcacctcgca gagcagcgag ccgacgccca
     2401 cccccaccga gaacagcacc agcagcagcg acgcgacatg gacgtcgccg tgcagcacgt
     2461 ccttggcgaa ggatgggaac tgcgacagga aggtggcgcc gaagaaccac atccacgaga
     2521 tgcccagcag cgaccggaac acgacgatgt ccgtgcgcgc cagcttcagg ttgcgcagcg
     2581 tctccgtcac cgggttccag ttgatcttga gcgcggtgtc gtgcgccggc gaggacggga
     2641 tggcctgggc cgtcaggcgc ccgatcaccg cgatggccag gcaggcgccg cccgccagct
     2701 ccggcccgat gcccggcacc accaccagca ggccgccggc gagctggccg agcaggatcg
     2761 cgacgaaggt gcccatctcg accatgccgt tgccgccggt gatctcgtgg tcgtccaggt
     2821 gctgcggcag gtaggcgtac ttgaccgggc cgaacagcgt cgagtgcacg cccatcagga
     2881 acaggcagcc cagcagcagc caggtcagct gcccgacgaa gccccagatc gccagcacca
     2941 tgatgaggat ctcgaacgac ttgatgaagc gcatcacccg cgccttgtcg tacttgtcgg
     3001 cgagctggcc gctggtggcc gacagcagaa cgaacggcag gatgaacacc gcgctgatga
     3061 cgaggcccgc cttctcgggc ggcagccagg atacgttcag ctggtacgtg acgagcaccg
     3121 tgaacgcgaa cttcaggacg ttgtcgttgc cggcgccgaa gaactgcgtc cagaagaacg
     3181 gcgcgaagcg gcgctggcgc agcagcgcga actggctggg atgggattgg ctcattgcag
     3241 tcgtgttctt ccgtgctcgg cgccgcgcgc cccgtggcgc cactgtagcc gcagcgccgc
     3301 cgggtcgacc ccggcagagc cctcgattcg gagacctttg gcgcgcgtca gcgcacgacc
     3361 gtcggccggc cggcgatctg catgcgctgc atccatgcga acatcgactc gacggtcacc
     3421 gtctcgatgt ggtcgctgaa gcgcttggag ttcgggtgat cgtcgaaggc gacgttcgcg
     3481 cgcgtcatcc gcgcacccag gcgcgtgagc gcgtcgatgt gcagcaccgt cggctggtag
     3541 tcgtgcagtt gcagccaggc ctgcgcgcgc tcgcgcgttg tcgcggccgg gtcgagcgag
     3601 ccggccagcg tctccagcgc ccactggttg ctctgctggt agcgggtcga ccacgggtag
     3661 gcgacgacgc tgtaggtcgg cgtgtgccac tgcaccaggc gctggtcgtc gacgacggtg
     3721 cgcagcagcg cggcctgcac ttccggatcg gggacgacgt acgcggcctc gtagcggaac
     3781 ggcgtgtcca ggaagaactc ggccaggccc tggcggaaca cggcggcatc ggccgtgccg
     3841 cattggttga gcttgtggac gatgcgccag gtgccgcggc gctggagcgg cacggcgaca
     3901 ggctggtcgg agatctggcc gaggacgggc gaggtcggca cggcgacggg cagcgcggcc
     3961 ggcgccggat cgtcgcggta ggcgaatgcc aggtgcgacc actgaaggcc gtacttggtc
     4021 aggtcctggc cggcgcgggc gatgatcacg acgcgcgccc cgctggcgtc caggctctgc
     4081 tgtacccgct cggccaggcc gagggcgcgc gtcattgcgt aggtgtcggg cggccgggta
     4141 tcgcaggtct ggccggcgcc ggccagcgtg gcgaccgcca atcccgcagc ggcggccagc
     4201 acgcgacccc agcggctcag gcggctcatc gcgacacccg ctggttgtac gacagggagc
     4261 ggccgatctc gttcgggatg aaggcgatca catggccggc tgcgctgagg agcatgcccg
     4321 tcgcgacgac cgtcacgacg accaccgttc cggccaccat cgccgaggcg ctgacggcgt
     4381 tggccgcgaa gcggatgctg cccttgacgc cgtcggcgac gttctcgacg aaccagacga
     4441 caccgtcggc actcgcctcg acccccgtca cgacgatcga gccggcgccg gcgacgagca
     4501 cgaccggcag cgcgaccgag atcgcgacgg gcaccagcga ttcctcgctg atgcggctgg
     4561 gctcgccttg cgcctgggtg gtgccggcag ccatgaggct caatgcgagc gcggaggcgg
     4621 cgatgcggga cgggagattt ggcccgggtt ccatgtgaaa cctcgaagtg gaagacgcgg
     4681 cgatggtgcg cagcagccga gcggccggca acgggattgc gccgagttcc gctgaccgca
     4741 tcgtgaggta tcgttcgcgg atactttcat cgaggctcca tgagcaccac cgccgagatc
     4801 gtcaacgtcc tcaagagcga gctgaagctg gccaagctca cctacgccga cctgggccgc
     4861 gagctcggcc tggcggagtc cagcgtcaag cggatgttcg ccaagggcga catgccgctc
     4921 aagcgcatcg acgagatctg ccgcgtcctc aggtgcgact tttcggagct ggcgcgcaag
     4981 gtcgccgacc tcgagctgct tcgcgacgag ctgacgctgg accaggagcg cgccgtcgtg
     5041 gcggacaagc ggctgctggt catggccctg tgcacgctga gccagtggcc gttcgagcag
     5101 atcatgggca tgtacgcctt cacggaggcc gaagcggtgc ggcacctgac gcagctggac
     5161 cggttgggca tcatcgagct caagccgctg aatcgctatc gcctcaaggt ggccaagaca
     5221 tttcgctggc gcgcgcacgg tccggtgatg cagttcttcc gcgatcacgg cgtggccgac
     5281 tacttctcgg gcggcttcaa cggcgagtgc gagatggtca gtctcgtgca cggccacctg
     5341 agcttgccga tggccaaggt gttccaggaa cggctgcagc gcgtcgccca ggatttcgcc
     5401 cagcagcacc tggccgacca gcgcctgccg gcagcgcaca agcggggctt cacggcggtg
     5461 atggcgttgc gctcgtggac cttcggcccg ctgcgcgagc tgctgcgcga cccgaatatc
     5521 gagccctgag cgggcgcgat cgccctgccc ggctcagcgg acgtcggcga gtgccgggcc
     5581 tttttcgcga gcgaccattt cctgcgcgaa ctcgacgaag gctaccgcgc cgcgggaagt
     5641 cgggtcgaac acgacacctg gctgaccgta gctgggggcc tccgccagac gaacgttgcg
     5701 cgggatgacg gtgttgaata ccttctcgcc gaagtgcgcc ttgagctgtt cgctgacgtg
     5761 cgcctgcagc gtgatgcgcg ggtcgtacat gacgcgcagc aggccgatga gttccaggtc
     5821 gcggttcagg ttggcgtgca cctgcttgat cgtgttgacc aggtcggaga cgccttcgag
     5881 cgcgaaatac tcgcactgca tcgggacgat gacgccgtgg gcgctgcaaa ggccgttgag
     5941 cgtcagcagg ttcagcgacg gcgggcagtc gatcaggacg aaatcgtaat cgccgtccac
     6001 ggccttgagg gcgcggcgca ggcgctcgtt gcggcgctcg agcgcgacca gttccacctc
     6061 ggcgccggcc agttcacgat tcgcgcccag cacgtcgtaa ccaccgctgc ccgacttctg
     6121 ggcggcttcg gtgatggtcg cgctctccag caggacgtcg tagaccgtca tcggc
//
LOCUS       DATCRO010000276         8494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_396776, whole genome shotgun sequence.
ACCESSION   DATCRO010000276 DATCRO010000000
VERSION     DATCRO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396776"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VIN75_07530"
     CDS             <1..610
                     /locus_tag="VIN75_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8974052.1"
                     /translation="AQKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRK
                     LAERSQVAAEEIANVASSSVELAEKAGHLLDTMVPNIRKTSDLVQEITAASEEQSAGV
                     SQINAAVTQLSQTTQQNASSSEELAATAEEMSSQAEQLQQTMSFFKVAGVAQNVRRGA
                     PLAPALRKVAASVRQAPRKSRIAAPVTPAAEADVDETNFARF"
     gene            663..1469
                     /locus_tag="VIN75_07535"
     CDS             663..1469
                     /locus_tag="VIN75_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY8974053.1"
                     /translation="MNTITDTEFTRFQRFIYDAAGITLSPAKKALVCGRLSKRLQANR
                     LESFGAYFELLSSGRASDEVQTAVDLLTTNETYFFREPRHFELLRELATQARDRPQPL
                     RVWSAASSSGEECYSIAMVLADCLDASQTSWDILGTDISTRVLRRARTGHYPLERTRN
                     IPPAYLKRFCLRGHGEQEGTLLVERALRSRVNFAQVNLNTDLPRLGTFDVVFLRNVMI
                     YFNGDTKRQVVARVLSALKPGGHLFIGHSESLNDISTAVDQLAPSIYRKP"
     gene            1466..2002
                     /locus_tag="VIN75_07540"
     CDS             1466..2002
                     /locus_tag="VIN75_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEY8974054.1"
                     /translation="MTTASPAPLRELFLQPGQFALGDASCRIRTVLGSCISIALWCPG
                     QRIGAMSHCLLPTRGREGVTRARGLELRGLDARYADEALQLMLQGLERRHVRAAQCSA
                     KIFGGGNMFPSQRAAGVPVGRRNGEAARGLLQAHGIEVVSESLFGDGHRQVVFDVATG
                     DVWARQMAPADAGPGPAA"
     gene            1999..3069
                     /locus_tag="VIN75_07545"
     CDS             1999..3069
                     /locus_tag="VIN75_07545"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY8974055.1"
                     /translation="MSVIKVMVVDDSAVVRQVVAGLLASAPGIEVLAAVSDPLLAIER
                     LRQSWPDVIVLDVEMPRMDGITFLRKIMAERPTPVVICSTLTEKGARTTLDALAAGAV
                     AIVTKPRLGLKQFLTESGDELVSTVRAAARANVRQVMPRADAAPLVASPKNTADVILA
                     PGGPRAMTQTTERVVALGTSTGGTQALEAVLSELPRVCPGIVVVQHMPEMFTAAFAAR
                     LDGICRIEVREARHNDRVMPGRALIAPGGRHLLLKRSGAQYFVEVIDGPMVNRHRPSV
                     DVLFRSVAKSAGANALGVIMTGMGDDGAAGLAEMRNAGARTLAQDEDSCVVFGMPKEA
                     IRRGGVERSVPLQGIAAEIMKQ"
     gene            3159..3485
                     /locus_tag="VIN75_07550"
     CDS             3159..3485
                     /locus_tag="VIN75_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8974056.1"
                     /translation="MKEDNVFAALASGPRRRILAYLSEAELTAGEIAERFDMTKPSLS
                     RHLRILEEAGLVKSDKRGQFVHYGLADDNLLASLHTMLMTFCPVGHPLKKESAAIARE
                     RRRKAT"
     gene            3523..4950
                     /locus_tag="VIN75_07555"
     CDS             3523..4950
                     /locus_tag="VIN75_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEY8974057.1"
                     /translation="MDTASAHPGTRLARRTRRTATFPFAALPIGPGWVPVEVSLVARL
                     GHGAGDKLYHEAALRQGRHGRFVNALDEPSAKLGNTDLARGDATALYSFGVGHRGHPF
                     HRHAGHRVFTAVAGSGGALLRFSSATPAELERDPGSFLRAMHVVSVPPDCLFTVRFSG
                     DTWHQFVSQDARGRHPAFFALSTHTNELGGDLSDALRQRVLAGEADIPSLTELLPETV
                     VRHLASAPRALAGAPVTALSLHDRPGGLLEAACRRVRSLAGRLQRWRIERGVRPGYVS
                     EVATTQVTEGTALAADSLLRDALPDRVVHHQDRFALALAGHDVAGVDAAAWLAAVLDG
                     FLANRPRGVARMMAWRNALVRPLGLRTSPLGCPVSSLLSTTASRRFAGRFPVHDFDVS
                     ADGRRAQVLLGADDKHLVFRSCVAVRRLDDGGVEFELSNRIACNNRFGRFYVAAIDWP
                     HRRFVAPTLLMHAVDAAVVALRAQR"
     gene            5234..7093
                     /locus_tag="VIN75_07560"
     CDS             5234..7093
                     /locus_tag="VIN75_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY8974058.1"
                     /translation="MKCRQHLACVALAAACAVLAAPAGAASLHQPGDGSDAVLGDGAA
                     TPETKAVYNLLKGLTLKANSNMLEGQHLGGPGDLVVDPGTQDMFDMQGYRIDSVSPGP
                     HAAYPRMVGARYDANVTPNQYVLDPALVAQINQKLVGIEAAYGPVISITATPLNPWNP
                     AAGRSYDTGDGALSKLFIANRGQNAAATAFWNDIDTIATGLANLKTADGTPIPVLFRP
                     FAEFNTDKYYFHKQSSADFVTLWQQVVDYYRGKQLHNLIICWEAWVWGSSSAMQPSQV
                     DIAPWYPGDSYVDVVSGAFYFQKNANVDNTPYFNLDIPPGSDDFNVFYNLTNLAVTHD
                     KPFGAAQWGLNYKSTPPGDDANTLAFMSAMDAKHANNANQPAGQKVQHMGFAYYWTGA
                     MQVQTQDNAAAFVNDPRVSSITGVQSLAAEDGWIQEANAGSNTGGSKGPAAYLQTGDS
                     GAATCKNCQLRSIVSFDTTSTLPAGATLVPSTPVTLLVTSQGTVGTSPFANAAAFGPL
                     CVDAAWTAATQAPGWFGAGNAFALDDFPLVAASNASCVATLPDPSLDAYGRSFANIGA
                     KFVNRAAGGRTQLRLRFTGATDNNNSNNYVSWYAGDAGRPNAPQLVFTTSLPN"
     gene            complement(7364..7954)
                     /locus_tag="VIN75_07565"
     CDS             complement(7364..7954)
                     /locus_tag="VIN75_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8974059.1"
                     /translation="MRLVHVVTHPEVRIDPAIPVTRWSLSDTGRARMQAFAAWPRLRR
                     ADRLFCSTEAKAVEAAQILQRSLGLSAHAVAALGENDRSATGFLHKEAFEQAADEFFA
                     HPAQSFRGWERAVDAQRRIVDAMERLLREAGAGDTVVVSHGAVGTLLKCHLKGCAITR
                     AEDQPAQGHVYTFEAATRRLLHDWRRLEDAAADAPA"
     gene            complement(8013..>8494)
                     /locus_tag="VIN75_07570"
     CDS             complement(8013..>8494)
                     /locus_tag="VIN75_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8974060.1"
                     /translation="LGLALAHPDGLARALSAGAGAPVATVTPLRAGRYAQASAALARG
                     RLAHPLLKFGLLPLLLAIPAFRLHQHIAYGGAFGEYYTFGLKAYLTTFALWWAAWAIA
                     VVLCAAAVRALIEAGTLAGVVVAPARAIAARRELERLGLAALYIGLPAWLLLRIVGA"
BASE COUNT         1271 a         3059 c         3007 g         1157 t
ORIGIN      
        1 cgcgcagaag atcggcatca tcgacgacat cgcctaccag acgaacctgc tggcgctgaa
       61 cgcggccatc gaggcggcgc gggcgggcga gcacggcaag ggcttcgcgg tcgtcgcggc
      121 cgaggtgcgc aagctggccg aacgcagcca ggtcgccgcc gaggagatcg ccaacgtcgc
      181 cagctccagc gtcgagctcg ccgagaaggc cggacacctg ctggacacga tggtgcccaa
      241 catccgcaag acgtcggacc tggtgcagga gatcacggcg gcctccgagg aacagtccgc
      301 gggcgtcagc cagatcaacg cggccgtcac ccagctgagc cagacgacgc agcagaacgc
      361 gtcgagctcg gaggaactcg ccgcgacggc cgaggagatg agctcgcagg ccgagcagct
      421 gcagcagacc atgtcgttct tcaaggtcgc cggcgtggcg cagaacgtgc gccgcggcgc
      481 gccgctggcg ccggccctgc gcaaggtggc ggcgtccgtg cgtcaagcgc cgcgcaagtc
      541 gcgcatcgcg gcgccggtga cgccggcggc cgaggccgac gtcgacgaga cgaacttcgc
      601 gcgattctga tgggcgcgtc catgaggcga acccgccggc cgggtgaacg ggacccactg
      661 cgatgaacac gatcaccgac accgaattca cccgcttcca gcgcttcatc tacgacgccg
      721 ccggcatcac gctgtcgccg gccaagaagg cgctggtatg cggccgcctg tccaagcggc
      781 tgcaggccaa ccggctggag agcttcggcg cctacttcga gctgctgtcc agcggccggg
      841 cgtcggacga ggtgcagacc gccgtcgacc tgctcaccac caacgagacc tatttcttcc
      901 gcgagcccag gcacttcgag ctgctgcgcg agctcgcgac gcaggcgcgc gaccggccgc
      961 agccgctgcg cgtgtggagc gccgcgagtt ccagcggcga ggagtgctac agcatcgcga
     1021 tggtgctggc cgactgcctg gacgcgtcgc agacgtcatg ggacatcctg ggcacggaca
     1081 tcagcacccg cgtgctgcgc cgcgcccgca cgggccacta cccgctggaa cgcacccgca
     1141 acatcccgcc ggcctacctc aagcgcttct gcctgcgcgg ccacggcgag caggagggca
     1201 cgctgctggt ggagcgcgcg ctgcgcagcc gcgtgaactt cgcgcaggtc aacctcaaca
     1261 cggacctgcc gcgcctgggc accttcgacg tcgtcttcct gcgcaacgtg atgatctact
     1321 tcaacggcga caccaagcgc caggtcgtcg cgcgggtgct gtccgcgctc aagccgggcg
     1381 gccacctgtt catcgggcac tccgagagcc tgaacgacat cagcacggcc gtcgaccagc
     1441 tcgcgccgtc gatctatcgc aagccatgac gacggcgtcg cccgcgccgc tgcgcgagct
     1501 cttcctgcaa cccgggcaat tcgcgctcgg cgacgcctcg tgccgcatcc gcacggtgct
     1561 gggctcgtgc atctcgatcg ccctgtggtg tccgggccag cgcatcggcg cgatgtcgca
     1621 ttgcctgctg cccacgcgcg gtcgcgaggg cgtgacgcgc gcgcgcgggc tggagctgcg
     1681 cgggctggac gcgcgctatg ccgacgaggc gctgcagctg atgctgcaag ggctggagcg
     1741 ccgccacgtg cgcgcggcgc agtgcagcgc caagatcttc ggcggcggca acatgttccc
     1801 gtcgcagcgc gcggccggcg ttcccgtggg gcgccgcaac ggcgaggccg cgcgcggcct
     1861 gctgcaggcg cacggcatcg aggtggtgtc cgagagcctg ttcggcgacg gccatcgcca
     1921 ggtcgtgttc gacgtggcca ccggcgatgt ctgggcacgc cagatggcgc cggccgacgc
     1981 gggcccgggg ccggcggcat gagcgtgatc aaggtcatgg tggtggacga ctccgcggtc
     2041 gtgcgccagg tcgtcgcggg gctgctcgcg tccgcgccgg gcatcgaggt gctggccgcg
     2101 gtgtccgatc cgctgctcgc gatcgagcgc ctgcgccagt cgtggccgga cgtcatcgtg
     2161 ctggacgtcg agatgccgcg catggacggc atcaccttcc tgcgcaagat catggccgag
     2221 cggccgacgc cggtggtgat ctgctcgacg ctgaccgaga agggcgctcg cacgacgctg
     2281 gacgcgctgg ccgccggcgc cgtggccatc gtgaccaagc cgcggctggg gctcaagcag
     2341 ttcctcaccg agtccggcga cgagctggtg tccaccgtgc gcgccgccgc gcgcgcgaac
     2401 gtgcgccagg tgatgccgcg ggcggacgcc gcgccgctcg tggcatcgcc caagaacacg
     2461 gccgacgtca tcctggcgcc cggcggtccg cgcgcgatga cgcagaccac cgagcgcgtc
     2521 gtggcgctgg gcacgtccac cggcggcacg caggcgctgg aggcggtgct ctccgagctg
     2581 ccgcgggtat gccccggcat cgtggtcgtg cagcacatgc ccgagatgtt cacggcggcc
     2641 ttcgcggcgc ggctggacgg catctgccgc atcgaggtgc gcgaggcgcg ccacaatgac
     2701 cgcgtcatgc cgggccgcgc gctgatcgcg cccggcggca ggcacctgct gctcaagcgc
     2761 agcggcgcgc agtacttcgt cgaggtgatc gacgggccca tggtcaaccg ccaccggcca
     2821 tcggtggacg tgctgttccg ctcggtggcc aagagcgccg gcgcgaacgc gctgggcgtc
     2881 atcatgaccg gcatgggcga tgacggcgcc gcaggactcg ccgagatgcg caacgccggt
     2941 gcccggacgc tggcgcagga cgaggacagc tgcgtggtct tcggcatgcc caaggaagcc
     3001 atccggcgcg gcggcgtcga gcgcagcgtg ccgttgcagg gcatcgccgc cgagatcatg
     3061 aagcagtagc gcgccgacgc ttgcggcatg gcgggcggcc tgctagcatg aggtatttag
     3121 ctaattggct aaataacgaa gcacatcgcc gacgccccat gaaggaagac aacgtctttg
     3181 ccgcgctggc ctcgggcccg cgccgccgca tcctcgccta cctgtccgag gcggagctca
     3241 cggccggcga gatcgccgag cgcttcgaca tgaccaagcc gtcgctgtcc aggcacctgc
     3301 gcatcctgga ggaggcgggc ctggtcaaga gcgacaagcg cggccagttc gtgcactacg
     3361 gcctggccga cgacaacctg ctggccagcc tgcacacgat gctgatgacg ttctgcccgg
     3421 tgggccatcc gctgaagaag gaaagcgcgg cgatcgcgcg cgagcgcagg cgcaaggcga
     3481 cctgaggcct tccttcaccg acgacggacg aggagcgacg acatggacac agcgtcggca
     3541 catcccggca cccggctcgc gcgccgcacg cgccgcaccg ccaccttccc gttcgccgcg
     3601 ctgcccatcg gcccgggctg ggtccccgtc gaggtgagcc tggtcgcgcg gctggggcat
     3661 ggcgccggcg acaagctgta tcacgaggcg gcgctgcgcc agggccggca cggccgcttc
     3721 gtcaatgcgc tcgacgagcc gtcggccaag ctgggcaaca cggacctcgc gcgcggcgac
     3781 gccacggcgc tgtactcgtt cggtgtcggc catcgcggcc atccgttcca tcgccatgcg
     3841 gggcaccgcg tgttcaccgc ggtcgcgggc agtggcgggg cgctgctgcg cttttccagc
     3901 gcgacgccgg ccgaactcga gcgcgacccc ggcagcttcc tgcgcgcgat gcacgtcgtc
     3961 agcgtgccgc cggattgcct gttcacggtg cgcttctcgg gcgacacctg gcaccagttc
     4021 gtctcgcagg acgcgcgcgg ccgccacccg gcattcttcg cgttgtccac gcacaccaac
     4081 gagctcggcg gcgacctgag cgacgcgttg cggcagcggg tgctggccgg cgaggccgac
     4141 atcccgtccc tgaccgaact gctgcccgag accgtcgtgc gccacctggc gtcggcgccg
     4201 cgggcgctgg cgggggcccc ggtcaccgcg ctgtcgctgc acgaccgccc cggcggcctg
     4261 ctggaggctg catgccgccg cgtgcgctcg ctggcggggc gcctgcagcg ctggcgcatc
     4321 gagcgcggcg tgcgtccggg ctacgtcagc gaggtggcca cgacgcaggt caccgaaggc
     4381 acggcgctgg cggccgattc gctgctgcgc gacgcgctgc cggaccgcgt cgtccaccac
     4441 caggatcgct tcgcgctggc gctggcgggg cacgacgtgg ccggcgtcga cgcggcggcg
     4501 tggctcgcgg cggtgctgga cggattcctc gcgaaccggc cgcgcggcgt ggcgcggatg
     4561 atggcctggc gcaacgcgct ggtgcgaccg ctcgggctgc gcacgtcccc gctcggctgc
     4621 ccggtgtcgt cgctgctctc gaccaccgcg tcgcgccgct tcgccgggcg cttcccggtg
     4681 cacgacttcg acgtctcggc cgacggccgg cgcgcgcagg tgctcctggg ggccgatgac
     4741 aagcacctcg tgttccgatc gtgcgtcgcc gtgcggcggc tggacgacgg cggcgtcgag
     4801 ttcgagctgt ccaaccggat cgcctgcaac aaccggttcg ggcgcttcta tgtcgccgcc
     4861 atcgactggc cgcatcgccg cttcgtcgcg cccaccctgc tgatgcacgc cgtggacgcc
     4921 gccgtcgtcg cgctgcgcgc gcaacgctga tcgcgccacg acggtccgga cggcatttgc
     4981 cggcggagtc cgacgatcta gggggagcca acataccgtt aacggtatgt gccaaaacct
     5041 tcacggtcgt cacctagagt cggcgcctgt gtcgacaggc ggtaccgcct gcagccgcaa
     5101 cgcccgttgg cgtccggcaa cgagagggat cgacatgacc gcgggacggc tcctgtgccg
     5161 cgccgctccc gggccgaggc gacacgatcg ccgcggaacc cgaccgtcat ccattcacga
     5221 agggagtttc ctcatgaagt gtcgacaaca cctcgcctgc gtggccctcg cggccgcctg
     5281 cgccgtgctc gccgcgcccg ccggcgccgc ctcgctgcac caacccggcg acggctcgga
     5341 cgccgtgctg ggcgacggcg cagcgacgcc ggagaccaag gccgtctaca acctgctcaa
     5401 gggcctcacg ctcaaggcca actccaacat gctggagggg cagcatctgg gcgggccggg
     5461 cgacctggtc gtggatccgg gcacgcagga catgttcgac atgcagggct accgcatcga
     5521 cagcgtctcg ccggggccgc atgccgcgta cccgcggatg gtcggcgcgc gctacgacgc
     5581 caacgtcacg cccaaccagt acgtgctcga cccggcgctc gtggcccaga tcaaccagaa
     5641 gctggtcggc atcgaggcgg cctacggccc ggtgatctcg atcaccgcga cgccgctgaa
     5701 tccgtggaac cccgccgcgg ggcggtcgta cgacacgggc gacggcgcgc tgagcaagct
     5761 gttcatcgcc aaccgcgggc agaacgcggc cgcgacggcc ttctggaacg acatcgacac
     5821 gatcgccacc ggcctcgcca acctgaagac cgccgacggc acgccgatcc ccgtcctgtt
     5881 ccggcccttc gccgagttca acaccgacaa gtactacttc cacaagcaga gcagcgccga
     5941 cttcgtcacg ctgtggcagc aggtggtcga ctactaccgc ggcaagcagc tgcacaacct
     6001 gatcatctgc tgggaggcct gggtctgggg cagcagcagc gcgatgcagc cgtcgcaggt
     6061 cgacatcgcc ccgtggtacc cgggcgactc gtacgtggac gtcgtctccg gcgcgttcta
     6121 tttccagaag aacgccaatg tcgacaacac gccgtacttc aacctcgaca tcccgcccgg
     6181 cagcgacgac ttcaacgtct tctacaacct gacgaacctg gcggtgacgc acgacaagcc
     6241 cttcggcgcg gcgcagtggg gcctcaacta caagagcaca ccccccggcg acgacgccaa
     6301 cacgctcgcg ttcatgtccg cgatggacgc caagcatgcc aacaacgcca accagccggc
     6361 gggacagaag gtgcagcaca tgggcttcgc gtactactgg acgggcgcga tgcaggtgca
     6421 gacccaggac aacgcggccg cgttcgtgaa cgacccgcgc gtgtcgtcca tcaccggcgt
     6481 gcagtcgctc gcggccgagg acggctggat ccaggaggcg aacgcgggct cgaacaccgg
     6541 cggctccaag gggccggcgg cctacctgca gacgggcgac tccggcgcgg cgacctgcaa
     6601 gaactgccag ctgcgcagca tcgtctcgtt cgacaccact tcgacgctgc cggccggcgc
     6661 gacgctcgtc cccagcacgc cggtgaccct gctcgtgacc tcgcagggca cggtcggcac
     6721 cagcccgttc gccaacgcgg ccgccttcgg cccgctgtgc gtcgacgcgg cctggacggc
     6781 ggccacgcag gcgccggggt ggttcggcgc cggcaacgcg ttcgcgctcg acgacttccc
     6841 gcttgtcgcc gccagcaacg cgagctgcgt ggcgaccttg cccgatccat cgctcgacgc
     6901 gtacgggcgc tcgttcgcca acatcggcgc gaagttcgtc aaccgcgccg ccggggggcg
     6961 cacgcaactg cgcctgcgct tcaccggcgc gaccgacaac aacaacagca acaactacgt
     7021 gagctggtat gcgggcgatg cgggcaggcc gaatgccccg cagctggtct tcacgacctc
     7081 gctgccgaac tgacgctggt gcacgacgtg catgccgtgc ccggtcgcga agggccgggc
     7141 agatcagcgg cttggccggc gagacgtcgt tgacgtcgat gacggcctcg tgctcgatgt
     7201 tcgcggcagt cgtgaggagg gtgtgatagt ggaggaactt gtccttgtcg ggctcgtctc
     7261 gcttcccgag gaagctcttg ccgggctcca gggtgccgat cgtgcggtcg cgcgggcgcg
     7321 gccacacccc tgtggatacg gtagggagtg ggcgcgctcg acgtcaggcg ggcgcgtcgg
     7381 cggcggcgtc ctccaggcgg cgccagtcgt gcagcaggcg gcgcgtggcc gcctcgaagg
     7441 tgtagacgtg gccttgcgcc ggctggtcct cggcgcgcgt gatcgcacag cctttcaggt
     7501 ggcacttcag cagggtgccg accgcgccgt gcgagacgac gaccgtgtcg ccggcgccgg
     7561 cttctcgcag caggcgctcc atggcgtcga cgatgcgccg ctgcgcgtcg acggcgcgct
     7621 cccagccgcg gaacgactgc gccggatgcg cgaagaattc gtccgccgct tgctcgaagg
     7681 cctccttgtg caggaagccg gtggcgctgc ggtcgttctc cccgagtgcc gcgacggcat
     7741 gcgcgctcag cccgagcgat cgctgcagga tctgcgccgc ctcgacggcc ttggcctcgg
     7801 tgctgcagaa cagccggtcc gcgcgccgca gccgcggcca ggcggcgaag gcctgcatgc
     7861 gcgcgcggcc ggtgtcggac agcgaccagc gcgtgacggg gatcgcgggg tcgatccgga
     7921 cttcggggtg agtgacgacg tggaccaggc gcatggctgc gggcgttgac ggcgagggcg
     7981 ccgattctgc cccgccgtgc gcatcctggc cctcatgcac ccacgatgcg cagcagcagc
     8041 cacgcgggca ggccgatgta cagcgccgcc aggcccagcc gctccagctc gcgccgcgcc
     8101 gcgatcgcgc gcgccggcgc gacgacgacg ccggccagcg tgccggcctc gatcagcgcg
     8161 cgcacggccg cggcgcacaa caccacggcg atcgcccagg cggcccacca cagcgcgaag
     8221 gtcgtcaggt acgccttcag gccgaaggtg tagtactcgc cgaacgcgcc gccgtacgcg
     8281 atgtgctggt gcagccggaa cgcggggatg gccagcagca gcggcaacag gccgaacttc
     8341 agcagcgggt gcgcgaggcg gccgcgcgcg agcgccgcgc tggcctgcgc gtagcggccg
     8401 gcgcgcagcg gcgtcacggt ggcgacgggc gcgccggcgc cggccgacag cgcgcgcgcc
     8461 aggccgtcgg ggtgcgccag cgcgagtccc aggc
//
LOCUS       DATCRO010000277         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_340501, whole genome shotgun sequence.
ACCESSION   DATCRO010000277 DATCRO010000000
VERSION     DATCRO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340501"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /gene="fadH"
                     /locus_tag="VIN75_07575"
     CDS             complement(<1..256)
                     /gene="fadH"
                     /locus_tag="VIN75_07575"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544654.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEY8974061.1"
                     /translation="MTSPYPKLLAPLDLGFTTLRNRTIMGSMHTGLEDAAKDFPRMAA
                     FYAERARHGCGLIVTGGFAPNFEGWVKPFAGTLWTHAGARR"
     gene            612..1976
                     /locus_tag="VIN75_07580"
     CDS             612..1976
                     /locus_tag="VIN75_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEY8974062.1"
                     /translation="MNKREFLAVGGAVPLVLAGCGGSGTGSAPVRLVNASVGYPNLGF
                     MVESTQETTTDVAYGHASSYINVQAGAVGITLTVGNNAATSAQTRTINKDQRYSLIAY
                     GYLNELKPLLVAESTITPDTGKANINVLNTSVDMGAVDVYLSTTADLSVATQIGTSVG
                     GVSQSAFTGVLAGSYFITVVGAGSVQQGISDVRFQTPAAVALTALKTYTVVLTPGASG
                     VLANAIILTQGTADAATPTAPYDNTTARVRAVTAVPGMAASVVGATAAGATVTVLSTS
                     TTQKYSKYFVVHTGTPPTVTVNGTAVPVKMDSTDSSGNPTTVAATLLAGGDYTILVYL
                     DGSGNPVAKAVEDNNTAAVTSAGVKFRLINLASDNQGLELSMSLNGVGIASNIPYGTA
                     SDYHEVTNVPQGTQSQLSVENGAASIITKNLVVPLGNVFTEIVVSVPATGSPLDFFGS
                     ASGN"
     gene            complement(2064..2978)
                     /locus_tag="VIN75_07585"
     CDS             complement(2064..2978)
                     /locus_tag="VIN75_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8974063.1"
                     /translation="MTTTLTAPTATPDAVVRKPRTVERLVAGQATSDGDGVKLTRVLT
                     QPLQRRLDPFLMLDAFGSDDAGDYIGGFPDHPHRGFETITYMIAGRMRHRDSTGNEGL
                     LQNGGVQWMTAGRGIIHSELPEQEQGRMEGFQLWLNLPAADKMTTPGYRDIPTGEIPE
                     LHLPGATVRVIAGDSHGVAGAVHKAHTEPLYLDLHLEPGATFEQPLPAGHNAFVYVYR
                     GELDVDGGAVRTAVPRERMAVLAHGADSDGVRVQAGAGGARALLIAGKPLNEPIAQYG
                     PFVMNKQAEIFQAVEDFRAGKFGTPPGR"
     gene            complement(3034..3663)
                     /locus_tag="VIN75_07590"
     CDS             complement(3034..3663)
                     /locus_tag="VIN75_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8974064.1"
                     /translation="MNILQINSSARPFANGQGSQSTRLAAELVETLRAGDADVTLTLR
                     DLAANPHPALDEATLGALFTPADARTPEQHARVAMDQALIDEVKAADVIVIGVPMINF
                     GISSQLKNWIDAIAKAGVTFKYTATGPVGLIEGKKVYAVLTRGGIYRDQPHDTQVPYL
                     RQTLGFLGIKDVEFIYAEGLNMGPEAADKGIASAREHIARIADGAAVAA"
     gene            3832..5175
                     /locus_tag="VIN75_07595"
     CDS             3832..5175
                     /locus_tag="VIN75_07595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8974065.1"
                     /translation="MQALIASQSGGSIRDQRAALLCLLALVVLSVASLMWNSLGMNQT
                     LVIDGLSPYPVRAVDDRDPSNHGSSVATVERHGRRLTLQCEVGTAAPYPFCSMRFTLA
                     PEPLGIDLSEFSVMRIWLDATGPESLQEVRIALGNFNPAYSRPNSYETLKNHELVYIQ
                     HEPHGVIEVPMDRMSVASWWIEERNLPLQYTGTELEHVVALDVVTGANLRAGAHRIAV
                     DRIELQRKLVSPAAFRLGLLLVWLAAVSLYVLVQLVNSKRQLLESRRQQQVLAQTITS
                     LNARSESLETLARSDPATGLLNRAGLKLGLEQRLLQHGLTQFPMAVIFVDIDHFKRIN
                     DGFGHATGDAVIAQFARHLRDHVPHDDLVARWGGEEFLLLCPGTAAEEALRIAERLRQ
                     DIERQPWPHALEVTCSFGITVLKAGEDWLQAIERADAAMYQAKQAGRNRVVMAQG"
     gene            5376..>6025
                     /locus_tag="VIN75_07600"
     CDS             5376..>6025
                     /locus_tag="VIN75_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974066.1"
                     /translation="MSPDADDLLLFARVAESGSFSRAAERVQLPKSTVSRRIAALEKR
                     LGERLLQRTTRKLVITEFGQGVLDHARAMSEEVDAALAFALSRQARPSGRLRVSMPGD
                     FASTALERPLAEFIRDFPEVSLELDLSPRRVDLIGENFDLAIRMGTLPDDSQLAARRL
                     AVFTTGLYAAPSLLREHGEPLAPEALHTMPALMLLSRSGEAVAWELSPREPATQGG"
BASE COUNT          893 a         2102 c         2119 g          911 t
ORIGIN      
        1 ggcggcgggc gccggcgtgc gtccacagcg tgccggcgaa cggcttgacc cagccctcga
       61 agttgggcgc gaagccgccg gtgacgatca gcccgcagcc gtggcgcgcg cgctccgcgt
      121 agaacgccgc catgcgcggg aagtccttgg cggcgtcctc caggccggtg tgcatgctgc
      181 ccatgatcgt gcggttgcgc agggtggtga accccagatc gagcggggcg agcagcttcg
      241 gatagggcga cgtcatcggg ttttctcgcg ggcgttggcg gctggaaggt gccgcatggt
      301 agagcggcgc gcgccgccag aatcggggcg gcgccctaaa ccgcaggccc tctcgccgaa
      361 cttccgggcg ctttggcact cgatgtccgg gcgcgacagc gtggctgcct gacatggggg
      421 caacagaccg gcaacacggc atgcctccaa tacgcgggtc gcaccgccga gctttcttgg
      481 gcagcatttc actggatgtc ccggcgcgac tgcgtcgctg ccgcgttgcg ggcaacagac
      541 cggcaacacg gcatgcccca taatgttgga ttcagatgac aggcgtccgc ctggaaaagt
      601 ggagtgtttc gatgaacaaa cgtgagttcc tggcagtggg cggcgccgtg cccctggtct
      661 tggccggctg cggcggcagc ggtacgggaa gcgccccggt tcgcctggtc aacgcgagcg
      721 tcggataccc caacctcggc ttcatggtcg agtccacgca ggagacgacc acggacgtcg
      781 cctatggtca cgcgagctcg tacatcaacg tgcaggccgg cgccgtcggc atcacgctga
      841 cggtcggcaa caacgccgcc acctcggcgc agacgcgcac catcaacaag gaccagcgct
      901 actcgctgat cgcctacggc tacctcaacg agctcaagcc cctgctggtc gccgaaagca
      961 ccatcacgcc cgacaccggc aaggccaaca tcaacgtgct gaacacgtcg gtggacatgg
     1021 gcgccgtcga cgtctacctg tcgaccaccg ccgacctgag cgtcgccacg cagatcggca
     1081 cctcggtggg cggcgtctcg cagagcgcct tcacgggcgt cctggcgggc tcgtacttca
     1141 tcaccgtcgt gggcgccggc tccgtgcagc agggcatcag cgacgtgcgc ttccagacgc
     1201 cggcggccgt cgcgctgacc gcgctgaaga cctacaccgt cgtcctgacg ccgggcgcca
     1261 gcggcgtgct cgccaacgcg atcatcctga cgcaaggcac ggccgacgcc gccacgccca
     1321 ccgcgccgta cgacaacacg acggcccgcg tgcgcgccgt caccgcggtc cccggcatgg
     1381 ccgcctccgt cgtcggcgcc accgccgccg gcgcgaccgt cacggtgctg tccaccagca
     1441 cgacgcagaa gtactcgaag tacttcgtgg tccacaccgg caccccgccg accgtcacgg
     1501 tcaacggcac ggccgtcccg gtcaagatgg attcgaccga ctcgtccggc aatcccacca
     1561 cggtcgcggc gacgctgctg gccggcggcg actacacgat cctggtctac ctggacggct
     1621 cgggcaaccc ggtcgccaag gccgtcgagg acaacaacac cgccgccgtc acgtcggccg
     1681 gcgtgaagtt ccgcctgatc aacctcgcgt ccgacaacca gggcctcgag ctgtcgatgt
     1741 cgctgaacgg cgtgggcatc gcctcgaaca tcccgtacgg cacggcgtcc gactaccacg
     1801 aggtgacgaa cgtgccgcag ggcacgcagt cgcagctgtc ggtcgagaac ggcgcggcca
     1861 gcatcatcac caagaacctg gtggtgccgc tcggcaacgt gttcacggaa atcgtcgtca
     1921 gcgtcccggc caccggctcg ccgctcgact tcttcggctc ggccagcggc aactgatcgc
     1981 cgccgtcgac ccgacacgaa aaaggcgccc gcgggcgcct tttttttgcg cccgcaggcg
     2041 ccttctcggc ctgaacgcgc ggctcagcgc cccggcggcg tgccgaactt gccggcgcgg
     2101 aaatcctcca cggcctggaa gatctcggcc tgcttgttca tcacgaacgg gccgtactgc
     2161 gcgatgggct cgttcagcgg cttgcccgcg atcagcaacg cgcgcgcacc gccggccccg
     2221 gcctgcacgc gcacgccgtc gctgtcggcg ccgtgcgcca gcaccgccat gcgctcgcgc
     2281 ggcaccgcgg tgcgcaccgc gccgccgtcg acgtccagtt cgccgcggta cacgtagacg
     2341 aacgcgttgt ggcccgcggg cagcggctgc tcgaacgtgg cgcccggctc caggtgcagg
     2401 tcgaggtaca gcggctccgt gtgcgccttg tgcaccgcgc cggccacgcc atggctgtcg
     2461 cccgcgatca cgcgcacggt ggcgccgggc aggtgcagct cggggatctc gccggtcggg
     2521 atgtcgcggt agccgggcgt cgtcatcttg tcggcggcgg gcaggttcag ccacagctgg
     2581 aagccttcca tgcggccctg ctcctgctcc ggcaactcgc tgtggatgat gccgcggccc
     2641 gcggtcatcc actgcacgcc gccgttctgc aacaggcctt cgttgcccgt cgaatcgcgg
     2701 tggcgcatgc ggccggcgat catgtacgtg atcgtctcga agccgcggtg cgggtggtcg
     2761 gggaagccgc cgatgtagtc gcccgcgtcg tccgagccga aggcgtcgag catcaggaac
     2821 gggtcgagcc gccgctgcag cggctgcgtg aggacgcgcg tcagcttgac gccgtcgccg
     2881 tcgctggtcg cctggcccgc gaccaggcgc tccaccgtgc gcggcttgcg cacgacggcg
     2941 tccggcgtcg cggtgggggc ggtcagggtc gtggtcatgg cagggtgctc ctttggatgc
     3001 ggcccggggg ccgcgaggtc gaatgaactc ggttcaggcc gcgacggccg cgccgtcggc
     3061 gatgcgggcg atgtgctcgc gggcggacgc gatgcccttg tccgcggcct ccgggcccat
     3121 gttcaggcct tcagcgtaga tgaactcgac gtccttgatg cccaggaacc cgagcgtctg
     3181 gcgcaggtag ggcacttgcg tgtcgtgcgg ctggtcgcgg tagatgccgc cacgagtcag
     3241 gacggcatag accttcttgc cctcgatcag gccgaccggc ccggtggcgg tgtacttgaa
     3301 cgtgacgccg gccttggcga tcgcgtcgat ccagttcttg agctggctgc tgatgccgaa
     3361 gttgatcatc ggcacgccga tcacgatgac gtcggcagcc ttgacctcgt cgatcagcgc
     3421 ctggtccatc gccacgcggg cgtgctgctc gggcgtgcgc gcgtcggcgg gcgtgaacag
     3481 ggcgcccagc gtcgcttcgt cgagcgcggg gtgcgggttg gcggcgaggt cgcgcagcgt
     3541 cagcgtcacg tcggcgtcgc cggcgcgcag cgtctcgacc agctcggcgg cgaggcgggt
     3601 ggattgcgag ccctggccat tcgcgaaggg gcgggcgctg gagttgatct ggaggatgtt
     3661 catggtggct cctgggtggg tggggcggtc agccaggatg gttggccgtt ggaatgaact
     3721 gtattgattc gccaacgcgt ggagaagatg tcaaagcgga gttcatcgtt ccgtcaatgg
     3781 aacaatggga gaggctggca accgcgtgga ggtcgagttc acaatgcagg aatgcaggca
     3841 ctgatcgcca gccagtccgg agggtcgatc cgcgatcagc gggccgccct gctgtgcctg
     3901 ctggcgctcg tcgtgctctc cgtggcctcc ctgatgtgga acagcctggg catgaaccag
     3961 acgctggtca tcgacgggct gtcgccctat ccggtacgcg ccgtcgacga tcgcgacccg
     4021 agcaaccacg gcagcagcgt cgcgaccgtc gagcgccacg ggcgccgcct gacgctccag
     4081 tgcgaggtcg gcacggcggc gccgtacccg ttctgctcga tgcgcttcac gctggcgccc
     4141 gagccgctcg gcatcgatct cagcgagttc tccgtcatgc gcatctggct ggacgccacg
     4201 ggccccgagt cgctgcagga ggtgcgcatc gcgctcggca acttcaaccc ggcttattcc
     4261 aggccgaaca gctacgagac gctgaagaac cacgagctcg tgtacatcca gcacgagccg
     4321 catggggtga tcgaggtgcc gatggatcgc atgagcgtcg cctcctggtg gatcgaggag
     4381 cgcaacctgc cgctgcaata caccgggacg gaactggagc atgtcgtggc gctcgacgtc
     4441 gtgaccggcg cgaacctgcg cgcgggcgcg catcgcatcg ccgtcgaccg catcgagctg
     4501 cagcgcaagc tcgtctcgcc ggcggcgttc cgcctgggcc tgctgctggt gtggctggcc
     4561 gcggtgagcc tgtacgtgct ggtgcagctg gtcaactcca agcgccagct gctggagagc
     4621 cgtcgccagc agcaggtgct cgcgcagacc atcacttcgc tcaacgcgcg ttcggaatcg
     4681 ctcgagacgc tcgcgcgctc cgaccccgcc accggcctgc tcaatcgcgc ggggctgaag
     4741 ctggggctcg agcagcggct gctgcagcac gggctcacgc agttccccat ggccgtgatc
     4801 ttcgtggaca tcgaccactt caagcgcatc aacgacggct tcggtcacgc gaccggggac
     4861 gccgtgatcg cgcagttcgc ccggcacctg cgcgaccacg tgccgcacga cgacctggtc
     4921 gcgcgctggg gcggcgagga gttcctgctg ctgtgtcccg gcacggccgc cgaggaggcg
     4981 ctgcggatcg cggagcggct gcggcaggac atcgagcgcc agccctggcc ccacgcgctg
     5041 gaggtgacct gcagcttcgg catcacggtg ctcaaggcgg gcgaggactg gctgcaggcc
     5101 atcgaacgcg ccgacgccgc gatgtaccag gccaagcagg cgggccgcaa ccgggtcgtg
     5161 atggcgcagg gctgacaacg ccggcgcgca ccgagccgcc ggccgccagg ccgatggcca
     5221 ggccgccccg cgagcgatcc ggcgcgcgct ctccctgcat gaacaggtcg aacacgtgcg
     5281 gcagcagcac aggcgcggcg cgacgtccgc cggcgcgccg atccggcgca ggcgaaaatg
     5341 ccgttgcgca cgcccgtgcg cgccgacttc ccgccatgag ccccgacgcc gacgacctcc
     5401 tgctgtttgc ccgcgtggcc gagtccggca gcttcagccg tgcggccgaa cgcgtgcagc
     5461 tgcccaagtc gacggtgtcg cgccgcatcg ccgcgctcga gaagcgcctg ggcgagcggc
     5521 tgctgcagcg cacgacgcgc aagctggtca tcaccgagtt cgggcagggc gtgctcgacc
     5581 acgcccgggc gatgtccgag gaggtggacg cggcgctcgc gttcgcgctg tcgcgccagg
     5641 cccgcccgag cgggcggctg cgcgtgtcga tgccgggcga cttcgcgagc accgcgctgg
     5701 aacggccgct ggccgagttc atccgcgact ttcccgaggt gtcgctggag ctggacctgt
     5761 cgccgcgccg cgtggacctg atcggcgaga acttcgacct ggcgatccgc atgggcacgc
     5821 tgcccgacga cagccagctg gccgcgcgcc ggctcgcggt gttcacgacg ggcctgtatg
     5881 ccgcgccgtc gctgctgcgc gaacacggcg agccgctggc gcccgaggcg ctgcacacga
     5941 tgccggcgct gatgctgctg tcgcgctcgg gcgaggcggt ggcgtgggag ctgtcgccgc
     6001 gcgagccggc gacgcagggc ggaga
//
LOCUS       DATCRO010000278         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_846951, whole genome shotgun sequence.
ACCESSION   DATCRO010000278 DATCRO010000000
VERSION     DATCRO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846951"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VIN75_07605"
     CDS             <1..691
                     /locus_tag="VIN75_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8974067.1"
                     /translation="SYVQGRPFSPSGVEWEQAVACWRNLHSDAGAHFDRVVDIDAATI
                     RPQVTWGTSPEMVLSIEDRVPDPEREKDDVKRGAMERALTYMALEPNKAIADIRIDKV
                     FIGSCTNSRIEDLREAAAVVRRVGGRIAENVRLAMVVPGSGLVKEQAEREGLDKVFTS
                     AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAL
                     HGHFVDARKYI"
     gene            814..1464
                     /gene="leuD"
                     /locus_tag="VIN75_07610"
     CDS             814..1464
                     /gene="leuD"
                     /locus_tag="VIN75_07610"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY8974068.1"
                     /translation="MQAFTLHTGLVAPMDRENVDTDAIIPKQFLKSIKRTGFGPNLFD
                     EWRYKDPGYPGQDPAARVPNPDFVLNQPRYQGASVLLARRNFGCGSSREHAPWALDQY
                     GFRAIIAPSFADIFFTNCFKSGLLPIVLPEDAVAKLFDEVNAFVGYKLTIDLPRQVVA
                     KPDGAEIPFDVQAFRKYCLVNGFDDIGLTLRHADKIRGYEAERLAKMPWLGHRLVG"
     gene            1478..1843
                     /locus_tag="VIN75_07615"
     CDS             1478..1843
                     /locus_tag="VIN75_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974069.1"
                     /translation="MAWPRALQLRRHFQRVVLGRPWLAFVVLGLAFCCFGMVTLNLLH
                     LLRANAELILDNGVMALADGGARQLVELVANGYLAMAAYVVMKTCEHALSHWLADTSA
                     FAPSRQDRADASTDSNETH"
     gene            1845..2954
                     /gene="leuB"
                     /locus_tag="VIN75_07620"
     CDS             1845..2954
                     /gene="leuB"
                     /locus_tag="VIN75_07620"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8974070.1"
                     /translation="MKIAILPGDGIGHEIIAEAVKVLHALELPFEAEFADVGGTAYAN
                     HGHPLPESTLALCKAADAILFGAVGDFKYDKLERHLRPEQAILGLRKHLQLFANLRPA
                     ICHPQLTHASSLKPELVSGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGADEAFDTM
                     RYSRPEIERIAHVAFQAARKRGRKVTSVDKANVLETFQFWRDVVTEVHAHYPDVELDH
                     MYVDNAAMQLVKAPKKLDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDAANKGLYE
                     PSHGSAPDIAGKGIANPLATILSAAMMLRYTLQQASAADRIESAVSSVLEAGLRTVDI
                     ASAGMRTVGTREMGDAVVAAIRAQAITTTTIKTTS"
     gene            3106..4233
                     /gene="asd"
                     /locus_tag="VIN75_07625"
     CDS             3106..4233
                     /gene="asd"
                     /locus_tag="VIN75_07625"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8974071.1"
                     /translation="MALVGLVGWRGMVGSVLMERMQAEGDFALIEPVFFSTSAVGGKA
                     PAQARNETTLKDAFDIDALKRCDIVITCQGGDYTTEVFPKLRAAGWNGHWIDAASSLR
                     MTDDSVIVLDPVNMPVIKNALAKGQRNWIGGNCTVSCMLMGVGALYKAGLVEWMSTQT
                     YQAASGGGAQHMRELLTQYGTLNASVRSLLDDPKSAILEIDRLVVEKQRALSAEETKN
                     FGVPLGGSLIPWIDKDLGNGTSKEEWKGMAETNKILGQGDGFGTPAVPVDGFCVRVGA
                     MRCHSQALTFKLKKDVPLADIEAMIAADNEWVKVVPNTREATLRDLTPVSVTGTMTIP
                     VGRIRKLAMGPEYLGAFTIGDQLLWGAAEPLRRMLRILLDK"
     gene            4688..>5694
                     /locus_tag="VIN75_07630"
     CDS             4688..>5694
                     /locus_tag="VIN75_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY8974072.1"
                     /translation="MVTKGRALNVMAAAALVLLSTGASALGLGRVNVQSVLGETLRAE
                     IEVTSLNAEEASSLRVSLASPEAFKASGVEYNAVLAGATIALQRRADGRPVLVLTSDK
                     VVQEPFVDVIVDFAWSTGHLTRSYTLLIDPPGRNTAAQPGTTATAPVFASPPPAPRAA
                     APAPAPAPQVALAPPPAPVQAPSPAPAARASKPAPAPKPTPAPKPAAAEREAPSPAGS
                     NAGESSVKVNPGDTLTSLAAANKPSGVSLDQMLVSLYRNNPSAFIGDNMNRMKSGVTL
                     QVPSAETAKQLSSEEAHQIIQAQSADFAEYRHRLAGGVSTVHTDEGKRVASGKVEAKV
                     N"
BASE COUNT          966 a         2006 c         1867 g          855 t
ORIGIN      
        1 gtcctacgtc cagggacgcc cgttctcgcc gtcgggcgtc gaatgggagc aggcggtggc
       61 ctgctggcgc aacctgcact cggacgcggg cgcgcacttc gaccgcgtcg tcgacatcga
      121 cgccgccacg atccgcccgc aggtcacctg gggcacgtcg ccggagatgg tgctgtcgat
      181 cgaggaccgc gtgcccgatc ccgagcgcga gaaggacgac gtcaagcgcg gcgcgatgga
      241 gcgggcgctg acctacatgg cgctcgagcc gaacaaggcg atcgccgaca tccgcatcga
      301 caaggtcttc atcggctcgt gcaccaacag ccgcatcgag gacctgcgcg aggccgccgc
      361 cgtggtgcgc cgcgtgggcg ggcgcatcgc cgagaacgtg cggctggcga tggtggtgcc
      421 gggctcgggc ctcgtcaagg agcaggccga gcgcgagggc ctggacaagg tcttcaccag
      481 cgccggcttc gaatggcgcg agccgggctg ctcgatgtgc ctggccatga acgccgaccg
      541 gctcgagccg ggcgagcgct gcgcgtccac cagcaaccgc aacttcgagg gccggcaggg
      601 cgccggcggc cgcacgcacc tggtgagccc ggcaatggcc gcggccgccg cgctgcacgg
      661 ccacttcgtc gacgcgcgca agtacatttg aacgacatga cgacccccgt ccgcggtgta
      721 ccgctgaccg ccccccgagg gggctgcggc cttgctccgg agcggccgag cgcggcggcc
      781 gcaggcagct cgacttcgta ggaatcttca accatgcaag cgttcaccct gcacaccggc
      841 ctcgtcgccc cgatggatcg cgagaacgtc gacaccgacg cgatcatccc caagcagttc
      901 ctcaagtcga tcaagcgcac gggcttcggg cccaacctgt tcgacgagtg gcgctacaag
      961 gatccgggct accccggcca ggatccggcc gcgcgcgtgc ccaatcccga cttcgtgctg
     1021 aaccagccgc gctaccaggg cgcctcggtg ctgctcgcgc gccgcaactt cggctgtggc
     1081 tccagccgcg agcacgcgcc ctgggcgctc gaccagtacg gatttcgcgc catcatcgca
     1141 cccagcttcg ccgacatctt cttcaccaac tgcttcaaga gcggcctgct gcccatcgtg
     1201 ctgccggaag acgcggtggc caagctgttc gacgaggtca acgccttcgt cggctacaag
     1261 ctcacgatcg acctgccgcg ccaggtcgtg gccaagcccg acggcgccga gatcccgttc
     1321 gacgtccagg cgttccgcaa gtactgcctg gtcaacggct tcgacgacat cggcctgacg
     1381 ctgcgccatg ccgacaagat ccgcggctac gaggccgagc ggctcgcgaa gatgccgtgg
     1441 ctgggccacc ggctggtcgg ctgaccggtg gacggacatg gcctggccgc gcgcgctcca
     1501 gctgcgacgc catttccagc gcgtcgtcct gggccgtccg tggctcgcgt tcgtcgtgct
     1561 gggcctggcg ttctgctgct tcggcatggt cacgctcaac ctgctgcacc tgctgcgcgc
     1621 gaacgcggaa ctgatcctcg acaacggggt catggcgttg gccgacggcg gggcgcggca
     1681 gctcgtcgag ctggtggcca acggctacct cgcgatggcc gcctacgtcg tcatgaagac
     1741 ctgcgagcac gcgctgtcgc actggctcgc cgacacctcc gccttcgcgc cgtcccgcca
     1801 ggaccgcgcg gacgcttcca ccgattccaa cgagacgcac tgacatgaag atcgcaatcc
     1861 tccccggcga cggcatcggc cacgagatca tcgcggaggc cgtcaaggtg ctccacgcgc
     1921 tcgagctgcc gttcgaggcc gagttcgccg acgtcggcgg caccgcctac gccaaccatg
     1981 gccatccgct gcccgaatcg acgctggcgc tgtgcaaggc ggccgacgcc atcctgttcg
     2041 gcgccgtggg cgacttcaag tacgacaagc tcgagcgcca cctgcgcccc gagcaggcga
     2101 tcctgggcct gcgcaagcac ctgcagctgt tcgccaacct gcgtccggcg atctgccatc
     2161 cccagctgac gcatgcgtcc agcctgaagc cggagctggt gtcggggctg gacatcctga
     2221 tcatccgcga gctcaccggc gacatctact tcggccagcc gcgcggccgc cgcaccgccg
     2281 tcgacggcca cttcccgggc gccgacgaag cgttcgacac gatgcgctac agccgccccg
     2341 agatcgagcg catcgcgcat gtcgcgttcc aggcggcgcg caagcgcggc cgcaaggtga
     2401 ccagcgtcga caaggccaac gtgctggaga ccttccagtt ctggcgcgac gtcgtcaccg
     2461 aggtgcacgc ccactatccg gacgtcgagc tcgaccacat gtatgtcgac aacgccgcga
     2521 tgcagctggt gaaggcgccc aagaagctgg acgtcgtcgt caccggcaac atgttcggcg
     2581 acatcctgtc cgacgaagcc gcgatgctca ccggctcgat cggcatgctg ccctcggcct
     2641 cgctggacgc ggccaacaag ggcctgtacg agcccagcca cggcagcgcg cccgacatcg
     2701 cgggcaaggg catcgccaat cctttggcta caatactgtc tgccgccatg atgctccgct
     2761 acaccctcca gcaagccagc gccgccgacc gaatcgagtc cgcggtgagc tcggtgctgg
     2821 aagcgggcct gcgcaccgtc gacatcgcgt cggcgggcat gcgcacggtc ggcacgcgcg
     2881 agatgggcga cgccgtggtc gcggccatcc gcgcccaggc gatcaccacc accacgatca
     2941 aaaccacgag ctgacgctcc gactcgcttc gcccctgctt cctccaagat ggttcccagg
     3001 cgacgcgagt cgcagggcgg gaagcaggac aagcggtggc tccggacgga gccttgatcg
     3061 acaacgttca atatgcgtgc agggtcacgc gacaaggcga acgacatggc attggtagga
     3121 ctcgtaggct ggcgcggcat ggtcggctcg gtgctgatgg agcggatgca ggccgaaggc
     3181 gacttcgcgc tcatcgaacc ggtcttcttc tcgacgagcg cggtgggcgg caaggccccg
     3241 gcgcaggcca ggaacgagac gacgttgaag gacgcgttcg acatcgacgc gctcaagcgc
     3301 tgcgacatcg tcatcacctg ccagggcggc gactacacca ccgaggtctt ccccaagctg
     3361 cgcgcggccg gctggaacgg ccactggatc gacgccgcgt cgtcgctgcg catgacggac
     3421 gactcggtca tcgtgctcga tcccgtcaac atgcccgtca tcaagaacgc gctcgccaag
     3481 ggccagcgca actggatcgg cggcaactgc accgtcagct gcatgctgat gggcgtgggc
     3541 gcgctgtaca aggcgggcct ggtcgagtgg atgagcacgc agacctacca ggcggcctcc
     3601 ggcggcggcg cgcagcacat gcgcgagctg ctgacgcagt acggcacgct caacgcatcg
     3661 gttcgctcgc tgctcgatga tcccaagagc gcgatcctcg agatcgaccg cctggtcgtc
     3721 gagaagcagc gcgcactgtc ggccgaggaa acgaagaatt tcggcgtccc gctgggcggc
     3781 tcgctgattc cctggatcga caaggacctg ggcaacggca cgtccaagga agaatggaag
     3841 ggcatggccg agaccaacaa gattctcggc cagggcgatg gtttcggcac gccggcggtt
     3901 cccgtcgatg gtttctgcgt gcgcgtcggc gcgatgcgct gccacagcca ggcgctcaca
     3961 ttcaagttga agaaggacgt gccgctggcc gacatcgaag cgatgatcgc cgccgacaac
     4021 gagtgggtga aggtggttcc gaatactcgc gaggcgacct tgcgcgacct cacgcccgtg
     4081 tccgtcacgg gcacgatgac gattcccgtc ggccgcatcc gcaaactcgc gatgggcccg
     4141 gaatatctcg gcgcattcac cattggcgac caattgctgt ggggagcggc cgagccgctg
     4201 cgccggatgc tgcgcattct tctcgacaag taatccacgc cgcaaggcgt tttcgggcgc
     4261 gcaacgtcgg gagatcgttg cgcgcacgcc aaaccctaca cacgaaatgt cttgttacgg
     4321 ttgagcgacc ggcgggacgc aggaaatacg gccgtgcgtg tcgtattcga gtattttcgg
     4381 ggttaactcc gcgcttgcgg cggccgcggc gcaggcaaaa tgcgcgcctg cgctggttaa
     4441 acccggcgcc gaagtgcgag aatattcgga cagggatcgg gccaaaacgg cggtcggttg
     4501 cgcaggcgaa ggcttcacgg atgagcttgt cagcgtatct gcctggtgct aaggtctttt
     4561 ccctgacgag cgtgggcctt tattgcccac gtctgtgacc gatgaactcg tcgagagatc
     4621 tgtcattcac ggcggtcttg ccgagatcgc ccatttgcat tcgggataca aattgaagaa
     4681 tcgctcgttg gtcaccaaag gtcgcgcgct gaacgtcatg gccgccgccg ccctggtcct
     4741 cctgtcgacc ggtgcttccg cactgggcct ggggcgcgtg aacgtgcaat ccgttctggg
     4801 cgagaccctg cgcgccgaaa tcgaggtgac gagcctgaac gcggaagagg cgtcttccct
     4861 gcgcgtgagc ctcgcgtctc ccgaagcctt caaggcgtcc ggcgtcgaat acaacgcggt
     4921 gctcgcgggc gccaccatcg cgctgcagcg tcgtgccgac ggccgtccgg tgctggtgct
     4981 gacgagcgac aaggtcgtcc aggagccgtt cgtcgacgtc atcgtcgact tcgcctggtc
     5041 caccggccac ctcacgcgtt cgtacacgct gctgatcgac ccgccgggcc gcaacaccgc
     5101 cgcccagccg ggcaccaccg ccaccgcgcc ggtgttcgca tcgccgccgc ccgcgccgcg
     5161 cgccgcggcc ccggcgcccg cgcccgcccc gcaggtcgcg ctcgcgccgc cgccggcacc
     5221 ggtccaggcg ccgtcgccgg cgccggccgc ccgcgcgtcc aagcccgcgc ccgcccccaa
     5281 gcccacgccg gcgcccaagc ccgcggcggc cgagcgtgaa gcgccgtctc cggccggcag
     5341 caacgcgggc gagtcctccg tcaaggtcaa ccccggcgac acgctgacgt cgctggccgc
     5401 cgcgaacaag ccgagcggcg tctcgctgga ccagatgctg gtctcgctgt accgcaacaa
     5461 cccgtccgcg ttcatcggcg acaacatgaa ccggatgaag tccggcgtca cgttgcaggt
     5521 gcccagcgcc gagaccgcca agcagctgtc gagcgaggaa gcgcaccaga tcatccaggc
     5581 ccagagcgcc gacttcgccg agtaccgcca tcgcctggcc ggtggcgtga gcacggtgca
     5641 caccgacgaa ggcaagcgcg tcgcgtcggg caaggtcgag gccaaggtca acga
//
LOCUS       DATCRO010000279         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_205670, whole genome shotgun sequence.
ACCESSION   DATCRO010000279 DATCRO010000000
VERSION     DATCRO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205670"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..2027
                     /locus_tag="VIN75_07635"
     CDS             336..2027
                     /locus_tag="VIN75_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8974073.1"
                     /translation="MTPPPSSSLALQRLYHWERTTPDRTVFTQPMGGGLLKEFTWKDM
                     VDESRRMAAFLRQQGFEPGARIAILSKNCAWWLMSDFAIWMAGYVSVPLYPTLAAGTI
                     TQILQHSEARLLFLGKLDDWEAMRPGVPAGLPCISYPLSPEDVQARVDMRWDEIVGRT
                     APLPGHPVREGGDLATIMYTSGTTGMPKGVMHSFDNFAWAIDAGLKRISLNSDARMLS
                     YLPLAHVAERALVEHGLLATGLHLFFAESLQTFARDLQRARPTTFFSVPRLWVKFQQG
                     IHAKMPPQRLERLLKIPFAGRLIRRKILKALGLDACRYAAGGAAPMPPDLLRWYSTLG
                     LDIIEVYGMTENCGVSHATLADSRRPGTVGLPYDGVESRLDPATGEIQVKTGCLMLGY
                     YKEPELSAAAFTADGWLHTGDKGALEADGWMRITGRVKDLFKTSKGKYVAPAPIEDKL
                     VTSPAIEACCVVGANYPQPFAMLMLNEDAAAKARDPAGRAALEDAMANHLLTVNHGLD
                     PHEQLEVLVLMTEPWTVENGLITPTFKVKRNVLETRYVANFDKWTSGAGKIVWAN"
     gene            2262..3821
                     /locus_tag="VIN75_07640"
     CDS             2262..3821
                     /locus_tag="VIN75_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8974074.1"
                     /translation="MPFQRLVLRAASPAAGVDAHRLRPRVFWPAVAAVCLLGLLLRLP
                     TLASRSLWLDETYSAWFSALTLHQLWTDVPRYETHPPMYYSMLAGWRQLFGASEAALR
                     SLSVLAGVLVIAVVGCAGRVLRSLPGRDVTALVAALFLAVNTGSVAYAQQARPYALET
                     LFATLAILAAWSLLERFLAGRDGTHDVSAPAATLAASTGATLWMHDTGAIVALGIWVG
                     MTVTLLVHTRGRHGREALVLAAAGLGALAIFSPFLARLAQQMSHVSATAFWVRAEPKD
                     LMAAWYLVTGGSDSTLAGFGALMLAGLALTWKRCRPQALFIAIVLLLPLSVVLAFSFL
                     VKPIFIDRLFEWMTPLAMLAAAIGAVAALPARAWRAAAIVVVTGLSLKGTYWLYGTQR
                     EDWRGIVQAIARQARPGDLVVVVPNELSVPLAYYARDPAFPQVLVLPGPFPALGLPRQ
                     YVANLGAPRIEPEDIATLRASLATHGRVWYIDRLADIYDVDDSVRREIAARHTLRSRT
                     KNLGIVWSLYD"
     gene            complement(3876..>4783)
                     /locus_tag="VIN75_07645"
     CDS             complement(3876..>4783)
                     /locus_tag="VIN75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY8974075.1"
                     /translation="RAQDKLGLPQGTIKATVLIETILAAFEMDEILYELREHSAGLNA
                     GRWDYIFSCIKKFKVDTDFCLAERSKVTMTSPFMRAYALLLLKTCHKRGAPAIGGMSA
                     LIPIKNDPEKNAIAMQGIINDKKRDAGDGYDGGWVAHPGLVEPAMKEFLAVLGDKPNQ
                     WEKQRDDVNVAAAQLLDFQPETPITEAGLRNNINVGIHYLGAWLAGNGCVPIHNLMED
                     AATAEISRSQVWQWIRSPKGVLDDGRKVTAEMVRPLVGEELARIKATGAVGQFDRAAQ
                     IFEKMSTDSAFAEFLTLPLYEEASF"
BASE COUNT          650 a         1728 c         1656 g          749 t
ORIGIN      
        1 gttccgggcg gcggcgccgt gcgcccaggc ccaggcaccc agtcgaagtc gtgcgagggc
       61 cagtgattcg gacaggttca tcgtcgaagc ggaagcggaa aggggatcgc gcccgctacg
      121 ctaacaggtc ggcgcgggcg acggcggccg acgtgcgcga cggccaaggg gagcgccagc
      181 aggcgtcgtg cccgccccgg gaccagcgcc gcgactgacc ttgcgggcca atgcgctggc
      241 cgggcgtccg ccgggccgcc gcggcgcggg ctttcaccat ggcgtcgcga gcgagtcccc
      301 actagtctgg ctcgagactt cgccgcacgc ccgtcatgac gccaccgccc tcgtcctccc
      361 tcgccctgca gcgcctgtac cactgggaac gcacgacgcc cgaccgcacc gtgttcaccc
      421 agcccatggg cggcgggctg ctcaaggaat tcacctggaa ggacatggtc gacgagtcgc
      481 gccgcatggc cgccttcctg cgccagcagg ggttcgagcc gggcgcgcgc atcgcgatcc
      541 tgtccaagaa ctgcgcgtgg tggctcatga gcgacttcgc gatctggatg gccggctacg
      601 tctcggtgcc gctgtatccg acgctggccg ccggcaccat cacgcagatc ctgcagcaca
      661 gcgaggccag gctcctgttc ctgggcaagc tcgacgactg ggaggcgatg cgcccgggcg
      721 tgccggccgg cctgccgtgc atcagctatc cgctgtcgcc cgaggacgtg caggcgcggg
      781 tggacatgcg ctgggacgag atcgtcggcc gcaccgcgcc cctgcccggc cacccggtgc
      841 gcgagggcgg cgacctggcc accatcatgt acacgtccgg caccaccggc atgcccaagg
      901 gcgtgatgca cagcttcgac aacttcgcct gggcgatcga cgccgggctg aagcgcatct
      961 cgctgaactc ggacgcgcgc atgctcagct acctgccgct ggcccatgtg gccgaacgcg
     1021 cgctggtcga gcacggcctg ctggccacgg gcctgcacct gttcttcgcc gagagcctgc
     1081 agaccttcgc gcgcgacctg cagcgggcgc gtccgaccac gttcttctcc gtgccgcgcc
     1141 tgtgggtcaa gttccagcag ggcatccacg cgaagatgcc gccgcagcgg ctcgagcgcc
     1201 tgctcaagat cccgttcgcc ggtcgcctga tccgccgcaa gatcctcaag gcgctgggcc
     1261 tggacgcctg ccgctacgcc gccggcggcg cggcgccgat gccgcccgac ctgcttcgct
     1321 ggtactccac gctgggcctg gacatcatcg aggtctacgg catgaccgag aactgcggcg
     1381 tatcgcacgc cacgctggcc gactcgcgcc ggcccggcac ggtggggctg ccgtacgacg
     1441 gcgtcgagtc gcggctggat ccggccaccg gcgagatcca ggtcaagacg ggctgcctga
     1501 tgctgggcta ctacaaggag cccgagctga gcgccgcggc gttcaccgcc gacggctggc
     1561 tgcacaccgg cgacaagggc gcgctggagg cggacggctg gatgcgcatc acgggccgcg
     1621 tgaaggacct gttcaagacc agcaagggca agtacgtggc gccggcgccc atcgaggaca
     1681 agctggtcac cagccccgcc atcgaagcct gctgcgtggt cggcgccaac tacccgcagc
     1741 cgttcgcgat gctcatgctc aacgaggacg cggccgccaa ggcgcgcgat ccggcgggcc
     1801 gcgccgcgct ggaggacgcg atggccaacc acctgctcac cgtcaaccac ggcctcgacc
     1861 cgcacgagca gctcgaggtg ctggtcctca tgaccgagcc gtggacggtg gagaacgggc
     1921 tgatcacgcc gacgttcaag gtcaagcgca acgtgctgga gacgcgctac gtggccaact
     1981 tcgacaagtg gacctcgggg gcgggcaaga tcgtctgggc gaactgacga cgtcggcgcg
     2041 gcccgaggcc gccgggcgcg cacgaggcgt catcctgcgc gcagtcgcag gatgcacctt
     2101 gccctgaggc acctcgttcg ccagcgtgga tcctgcgact tcgcgcagga tgacacgtga
     2161 ttggcgcccg cggcgggcgc gggccccgac gccggcacca tgcaccgcta acatcccggc
     2221 gggctcgtcg cccgaccttg cccggatcct gcctcgctgc cgtgccgttc caacgcctcg
     2281 ttcttcgcgc tgcctcgccc gctgccggcg tcgacgccca ccgcctgcgc ccgcgcgtgt
     2341 tctggccggc cgtcgccgcc gtctgcctgc tgggattgct gctgcgactg ccgacgctgg
     2401 ccagccgctc gctgtggctg gacgagacgt acagcgcctg gttctcggcg ctgacgctgc
     2461 accagctgtg gacggacgtg ccgcgctacg agacgcatcc gccgatgtac tactcgatgc
     2521 tggccggctg gcgccagctg ttcggcgctt cggaggccgc gctgcgctcg ctgtcggtgc
     2581 tggcaggcgt gctcgtgatc gccgtcgtgg ggtgcgccgg gcgcgtgctg cgctcgctgc
     2641 cgggccgcga cgtcaccgcc ctggtggccg cgctgttcct ggcggtcaac accggcagcg
     2701 tcgcctatgc gcagcaggcg cgcccctacg cgctcgagac gctgttcgcc accctcgcga
     2761 tcctcgccgc atggtcgctg ctggaacggt tcctggcagg ccgcgacggc acgcatgacg
     2821 tgtcggcccc ggccgccacg ctggccgcgt cgaccggcgc cacgctctgg atgcacgaca
     2881 cgggcgccat cgtcgccctc ggcatctggg tcggcatgac cgtgacgctg ctcgtgcaca
     2941 cgcgaggccg gcacggccgc gaggcgctgg tgctggccgc ggcgggcctg ggcgccctgg
     3001 ccatcttctc gcccttcctg gcgcgcctgg cccagcagat gtcgcacgtc tcggccacgg
     3061 cgttctgggt gcgcgcggag ccgaaggacc tgatggccgc gtggtacctc gtcaccggcg
     3121 gcagcgactc gacgctcgcc ggcttcggcg cgctgatgct ggccggactc gcgctgacgt
     3181 ggaagcgatg ccggccccag gcgctgttca tcgccatcgt gctgctgctg ccgctgtcgg
     3241 tggtgctggc cttcagcttc ctggtcaagc cgatcttcat cgaccgcctg ttcgagtgga
     3301 tgacgccgct ggccatgctg gccgcggcca tcggcgcggt ggccgccctg cccgcgcgcg
     3361 cctggcgcgc cgccgccatc gtggtggtga cgggcctgag cctgaaaggc acgtactggc
     3421 tctatggcac gcagcgcgag gactggcgcg gcatcgtgca ggcgatcgcg cgacaggccc
     3481 ggcccgggga cctggtcgtc gtggtgccca acgagctgag cgtgccgctg gcctactatg
     3541 cccgcgaccc ggcgttcccg caggtgctgg tcctgcccgg cccgttcccg gcgctgggcc
     3601 tgccgcgcca gtacgtcgcc aacctcggcg cgccgcgcat cgagcccgag gacatcgcca
     3661 cgctgcgcgc gagcctggcc acgcacgggc gcgtctggta catcgaccgc ctggccgaca
     3721 tctacgacgt cgacgacagc gtgcgccgcg agatcgccgc gcgccacacg ctgcgcagcc
     3781 ggaccaagaa cctgggcatc gtctggtcgc tgtacgactg acgcgcatga aaaaggccgc
     3841 ccgcgggcgg cctggtcgca acgcgcggac gtcgatcaga acgaggcttc ctcgtacagc
     3901 ggcagcgtca ggaactcggc gaaggccgaa tcggtgctca tcttctcgaa gatctgcgcg
     3961 gcgcggtcga attgcccgac ggcgccggtc gccttgatgc gcgcgagctc ctcgcccacc
     4021 agcggacgca ccatctcggc cgtcaccttg cggccgtcgt ccagaacgcc cttggggctg
     4081 cggatccact gccagacctg cgagcggctg atctcggcgg tggccgcgtc ctccatcagg
     4141 ttgtggatcg gcacgcagcc gttgccggcc agccaggcgc ccaggtagtg gatgccgacg
     4201 ttgatgttgt tgcgcaggcc cgcttcggtg atgggcgtct cgggctggaa atccagcagc
     4261 tgggccgctg ccacgttgac gtcgtcacgc tgcttttccc actggttggg cttgtcgccc
     4321 agcaccgcca ggaactcctt catcgcgggc tcgaccaggc ccgggtgcgc cacccagccg
     4381 ccgtcgtagc cgtcgccggc gtcgcgcttc ttgtcgttga tgatgccctg catcgcgatg
     4441 gcgttcttct ccggatcgtt cttgatcggg atcagcgcgc tcatgccgcc gatggccggc
     4501 gcgccgcgct tgtggcaggt cttgagcagc agcagcgcgt aggcgcgcat gaacggcgac
     4561 gtcatcgtga ccttggagcg ctcggccagg cagaagtcgg tgtcgacctt gaactttttg
     4621 atgcagctga agatgtagtc ccagcggccg gcgttcaggc ccgcgctgtg ttcgcgcagc
     4681 tcgtacagga tctcgtccat ctcgaacgcg gcgaggatgg tctcgatcag cacggtggcc
     4741 ttgatcgtgc cctgcggcag gcccagcttg tcctgggcgc gca
//
LOCUS       DATCRO010000280         8823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_790742, whole genome shotgun sequence.
ACCESSION   DATCRO010000280 DATCRO010000000
VERSION     DATCRO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790742"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VIN75_07650"
     CDS             complement(<1..440)
                     /locus_tag="VIN75_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HEY8974076.1"
                     /translation="MEQALAPAVPLAVTPGPLDRMMAMPASAKMKLGAGVAGLAAVIV
                     AICLWSSQGDYRVLFANLPDKEGGAVVAQLATMQVPYKFAEGGTAILVPADKVNEVRL
                     KLASAGLPKSSVVGFELMDNAKFGQTQTQERVNLQRALEGELTRT"
     gene            716..1078
                     /gene="fliE"
                     /locus_tag="VIN75_07655"
     CDS             716..1078
                     /gene="fliE"
                     /locus_tag="VIN75_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY8974077.1"
                     /translation="MDVRLKPFDFAAAVARAGLKTDGTPIAGGSSPLGSSAGATGAGA
                     AGKAGFGTALSDALKGVSDLQNQASWMQNELQMDNPTVSLEQTMVTMQKAQIGFQAAI
                     SVRNRLVQSYTDIMNMQV"
     gene            complement(1220..1540)
                     /locus_tag="VIN75_07660"
     CDS             complement(1220..1540)
                     /locus_tag="VIN75_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974078.1"
                     /translation="MSSNARLVQRVGLAVAIIALVLAVAAWLRPGPDDEVDAVEHQRE
                     MQQLERLGGRASVRTAEFDQWLSSLWHGRRLAATLAVLGLVVGGACWRIGALMAEDDS
                     ASEA"
     gene            complement(1802..3832)
                     /locus_tag="VIN75_07665"
     CDS             complement(1802..3832)
                     /locus_tag="VIN75_07665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8974079.1"
                     /translation="MRGLRYFGGSLKIRLALAGLVLIAASVALTVVLVLRAMEQRSQR
                     SALDSEVANAERIGLVLSTKIVSLQNSLRAATSQLPVAQLADPDAVRHWLDGQRVLSS
                     LFDGVFLADGRLHVIGFAENGAVRSTDIDASDRDYLRRTVRERRSIVSRVGVSRTSGT
                     PMVAITMPVFDADGHLAAVLIGGMKLSSNALLNDLTRPAMDDHNPLATIIADAEANVV
                     SSADPSRVMTKVVADPHFAGVVAQWRAEGAPIEPQGSARHLGDDIVAVAGVPAADWVV
                     FRSAPAEVLLGGPTAGRRQAIWIGSAVAVAGGLIILLVMLALLRPLRQLEKRALRLIS
                     EGFSAQEPWPRAGGELGELTRVFQYVLRQKAEWRHASDELLGKMRAVMSTAPVGIAFT
                     RMGRFELVSDEFARLFGYDSARVHGMQTHVICPSDEAHREFIRMAAETFAAGGTCDAE
                     RELVRADSSRFWARLQGAPVRVGDASAGTIWIFTDITESRRHREQLSWSASHDELTRL
                     ANRREFELRLAGEIGERAGAEPSAALFIDLDRFKAVNDGSGHAAGDVLLKDIAAILLT
                     RARAHDTVARIGGDEFAVLLRGCDARAAERIAAGICMRVAEHTLVWSSGGRSQLLSVG
                     ASIGIVAIDATFDSVKQVLEAADEACYEAKRAGRGTVRMYRRAAGAATLAAG"
     gene            complement(3865..4626)
                     /locus_tag="VIN75_07670"
     CDS             complement(3865..4626)
                     /locus_tag="VIN75_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY8974080.1"
                     /translation="MFEDTRPASLDALGSGDGLAEFRVSAPGEIRALLKQLMDEVVPL
                     NLSASDGSAYTTTLWTIDAANARVSFTADLQQPAVHDIVEADECVAVAYLDRIKVQFD
                     VGDLVLVQGPKASVLQARLPRELFRFQRRNTFRVRTIERTLPTAQFRHPGIPDMSVAL
                     RVLDVSIGGCALLQPSNVPGLQPGAVIKGVRLSLDADTQIDAGLMLHHVTSTGAENGS
                     VRLGCEIVGMSPTAQRALQVYIDQTQKRRRLMPNE"
     gene            complement(4755..5060)
                     /locus_tag="VIN75_07675"
     CDS             complement(4755..5060)
                     /locus_tag="VIN75_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEY8974081.1"
                     /translation="MSTKLLNYYEAIEKASAEMLAAARAGNWDEVVKLEGACAVLIAQ
                     LKNAAQSTQLMQTEAKEKSRIMQRILLNDAEIRHLAEPWLEDLDQMMGGGSRPAFLH"
     gene            complement(5093..5527)
                     /gene="fliS"
                     /locus_tag="VIN75_07680"
     CDS             complement(5093..5527)
                     /gene="fliS"
                     /locus_tag="VIN75_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY8974082.1"
                     /translation="MFSAATPSAFGRSYGFANAYARVGVETSVNAASAHKLIELLFNG
                     FMDSIVSARGAMRAGQIEAKGKAIGRAARIVEEGLKAGLNMEGGGRLAADLSSLYQYV
                     GLRLLQANLRNDEDALDECARLLGPLRDAWVAIGPQVEAGNL"
     gene            complement(5705..7207)
                     /gene="fliD"
                     /locus_tag="VIN75_07685"
     CDS             complement(5705..7207)
                     /gene="fliD"
                     /locus_tag="VIN75_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY8974083.1"
                     /translation="MTTTTNLSSTTGSISSAGIGSGLDVKSIITSLMAVESQPLTLLQ
                     QKAASVQTEISAVGQIQSLTSTLSDKAHALSSSTLWTQTTSTSADTTAVTADTANGTA
                     APGDYSVSVQQLAQGQTVTSPAAAGSTLNAGTISIQLGTYKSDGATPPTVTFEAASSP
                     KVDIPIGASQTSLSAIRDQINSYNAGVQASIITDANGSRLSLRSTSTGAANAFQVTTT
                     ETTDDGVAGTGLSALNFDASTAATNSQMVLNQSAVNAKATVNGIDIESASNKLSNISD
                     GLSLTLLRKTTDPVDVTVAADTGSMQKAITDFVSAYSALQSYISLQTKYDASTAVAQG
                     TSRQDGPLQGDPSIVGFQNQLRGIINTTSTTSSMFARLSDIGIAVQTDGTLAVSSPNK
                     MTSALAHPDELAKLFSTWGTTNENTGIAQRFAALADKANATDGSLFSRTAGLKGELSR
                     NQQQQDDMQTHLDATQERLTAQYQALDTTMSKMNALSSYVTQQIAVMNKS"
     gene            complement(7417..8268)
                     /locus_tag="VIN75_07690"
     CDS             complement(7417..8268)
                     /locus_tag="VIN75_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin domain-containing protein"
                     /protein_id="HEY8974084.1"
                     /translation="MPLTINTNIASLNAQRNLNMSQGSLATSMQRLSSGLRVNSAKDD
                     AAGLAIAERMNAQSRGMNVAIRNANDGISMSQTAEGALSQVSNSLQRMRELAVQARNA
                     TNSSSDKDSLNKEFSQLQQEISRVLAGTTFNGKHILGADATALTFQIGANTTADDTIT
                     IATTDMSMDTTITAVTNTTTALIDASATLGAIGTVIDNIDKALDSVNDTRATYGATQS
                     RFDAIITNLQVGVENQSAARSRIMDADFASETANMSRAQVLQQAGTAMVAQANQLPQQ
                     VLKLLQG"
     gene            complement(8656..>8823)
                     /locus_tag="VIN75_07695"
     CDS             complement(8656..>8823)
                     /locus_tag="VIN75_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8974085.1"
                     /translation="QSSVENQTAARSRIMDADFATETANMSRANVLQQAGTAMIAQAN
                     QQPSQVLSLLR"
BASE COUNT         1251 a         2928 c         3172 g         1472 t
ORIGIN      
        1 gtgcgggtga gctcgccctc gagcgcgcgc tgcaggttca cgcgctcctg ggtctgggtc
       61 tggccgaact tggcgttgtc catcagctcg aagccgacga cactggactt gggcagcccg
      121 gcggaagcca gcttcaggcg cacctcgttg accttgtcgg cgggcaccag gatggcggtg
      181 ccgccctcgg cgaacttgta gggcacctgc atcgtggcca gctgggcgac gacggcgccg
      241 ccttccttgt ccggcaggtt ggcgaacagc acgcggtagt cgccttgcga gctccacagg
      301 cagatcgcca cgatcaccgc ggccaggccg gcgacgccgg cgcccagctt catcttcgcg
      361 ctggcgggca tcgccatcat gcgatcgagc gggccgggcg tgacggcgag gggaacggca
      421 ggggcgagag cttgttccat ggaggcggcg agagagggct ggtttgttcg ctggcgccgg
      481 acagggaaag acccagcctc ggcgcgatgg aagcattctt tagcctcgac ctatcgggcg
      541 aagccacgag aaaggcgcca ttcggccagc agatctcttc ttctgttaca ggcccgatcg
      601 ctaaagttca gaccatgcca gccgaacccg tcctccgcct cgcacgcgcc ctgcccgcct
      661 tcccggcggc cggccgtcgc ccgcgcgcgg gggcgacgag gagcgcctca tgacgatgga
      721 cgtccgactc aagcccttcg acttcgccgc cgccgtcgcg cgcgccggcc tgaagacgga
      781 cggcaccccg atcgccggcg gttcgagccc gctcggctcg tcggcgggcg ccaccggcgc
      841 gggagccgcc ggcaaggccg gcttcggcac ggccctgtcg gacgcgctca agggcgtcag
      901 cgacctgcag aaccaggcca gctggatgca gaacgagctg cagatggaca accccaccgt
      961 cagcctcgag cagacgatgg tgacgatgca gaaggcccag atcggcttcc aggccgcgat
     1021 ctcggtgcgc aaccggctgg tgcagtccta cacggacatc atgaacatgc aggtctgagc
     1081 ctcccgctcg gctccgctga ggcgcccgcg ggcgccttct cgcattccgg cgaccgttcc
     1141 ggggtccgac cgttccgggg tcaggtcttg aacgttgcgg ggtcaggtct tgaatcgtgc
     1201 agtcggcaat gctgcgggct caagcctcgc ttgcgctgtc gtcttcggcc atcagtgcgc
     1261 cgatgcgcca gcaggcgccg cccacgacca ggcccagcac cgccagcgtg gcagccaagc
     1321 gccgcccgtg ccacagcgag gacagccact ggtcgaactc cgccgtccgt acgctggccc
     1381 ggccgcccag ccgttcgagc tgctgcatct cgcgctggtg ctcgacggcg tcgacttcgt
     1441 cgtccggccc ggggcgcagc caggcagcga cagccagcac gagggcgatg atggcgacgg
     1501 cgaggccgac gcgctggacg aggcgggcgt tcgaagacat gggatcgcat tgtcgccgcg
     1561 cgacggcgca tcgacgcccg gggtcaggtc ttgaatcgtg cgctttgccg acggcgcgat
     1621 ggccgactgc atgattcaag acctgacccc gcaacgttca agacctgacc ccgggagaat
     1681 gcgccaagct tccacactcg atggcatcgc tgtgcccgta cgtcttccgc ttcagtggcg
     1741 tcccccggcg tagatggccg tcgttgagga acacgcgctg ttcggtgtcg atgcgcgagc
     1801 gtcagccggc cgccagcgtc gccgccccgg ccgcacgccg gtacatgcgc accgtgcccc
     1861 gccctgcccg cttggcctcg tagcacgcct cgtcggcggc ctccagcacc tgcttgaccg
     1921 agtcgaaggt ggcgtcgatg gcgacgatgc cgatgctcgc gcccacggac agcagctgcg
     1981 accggccgcc gctgctccag acgagcgtgt gctcggccac gcgcatgcag atgcccgcgg
     2041 cgatgcgctc ggccgcgcgc gcgtcgcagc cgcgcagcag cacggcgaac tcgtcgccgc
     2101 cgatgcgcgc gacggtgtcg tgcgcgcggg cgcgcgtgag caggatggcg gcgatgtcct
     2161 tgagcagcac gtccccggcg gcgtggccgc tgccgtcgtt gacggccttg aagcggtcca
     2221 ggtcgatgaa cagcgcggcc gacggctcgg cgccggcgcg ctcgccgatc tccccggcca
     2281 ggcgcagctc gaactcgcgg cggttggcca ggcgcgtgag ctcgtcatgc gaggccgacc
     2341 acgacagctg ctcgcgatgg cggcggctct cggtgatgtc ggtgaagatc cagatcgtgc
     2401 cggcgctggc gtcgcccacg cgcacgggcg cgccctgcag ccgcgcccag aagcggctgg
     2461 agtccgcgcg cacgagctcg cgctcggcgt cgcaggtgcc gccggcggcg aaggtctcgg
     2521 cggccatgcg gatgaactcg cgatgcgcct cgtcggacgg gcagatcacg tgggtctgca
     2581 tgccgtgcac gcgcgcgctg tcgtagccga acaggcgcgc gaactcgtcg ctcaccagct
     2641 cgaagcggcc catgcgcgtg aaggcgatgc ccaccggcgc cgtggacatc accgcgcgca
     2701 tcttgcccag cagctcgtcg ctcgcgtgac gccactcggc cttctggcgc agcacgtact
     2761 ggaacacgcg cgtcagctcg cccagttcgc cgccggcgcg cggccagggc tcctgcgcgg
     2821 agaagccctc gctgatcagg cgcagcgcgc gcttctcgag ctggcgcagc gggcgcaaca
     2881 gcgccagcat caccagcagg atgatcagcc cgccggccac ggcgacggcc gagccgatcc
     2941 agatcgcctg gcggcgtccc gcggtcggac cgcccagcag cacctcggcg ggcgcgctgc
     3001 ggaacaccac ccagtcggcc gcgggaacgc ccgccacggc cacgatgtcg tcgcccaggt
     3061 gccgcgccga accctgcggc tcgatcggcg cgccctcggc ccgccactgc gccacgacgc
     3121 ccgcgaagtg cggatccgcg acgaccttgg tcatcacgcg ggacgggtcg gcgctggaga
     3181 cgacgttggc ctcggcgtcg gcgatgatcg tcgccaacgg gttgtggtcg tccatcgccg
     3241 gccgcgtcag gtcgttgagc agcgcattgg acgacagctt catgccgccg atcagcaccg
     3301 cggccaggtg gccgtcggcg tcgaacaccg gcatcgtgat cgccaccatc ggcgtgccgc
     3361 tggtgcggct gacgcccacg cgcgacacga tgctgcggcg ctcgcgcacc gtgcggcgca
     3421 ggtagtcgcg gtcggacgcg tcgatgtcgg tgctgcgcac ggcgccgttc tcggcgaagc
     3481 cgatcacgtg caggcggccg tcggcgagga aaacgccgtc gaacaggctg gacagcacgc
     3541 gctgcccgtc gagccagtgc ctgacggcgt cggggtcggc cagctgcgcg acgggcagct
     3601 gcgacgtcgc cgcgcgcagc gagttctgca acgacacgat cttggtcgac agcaccaggc
     3661 cgatgcgctc ggcgttggcg acctccgaat cgagggccga acgctggctg cgctgctcca
     3721 tggcgcgcag cacgaggacg acggtgagcg cgacgctcgc cgcgatgagc accaggcccg
     3781 cgagcgcgag ccggatcttg agcgagccgc cgaaatagcg cagaccacgc atcgaggatg
     3841 tccgacagga agcagcgccg cgcgctactc gttgggcatc agccgccggc gcttctgcgt
     3901 ctggtcgatg tagacctgca atgcacgctg cgccgtcggg ctcatgccga cgatctcgca
     3961 cccgagccgc acggagccgt tctcggcgcc ggtggaggtg acgtggtgca gcatgagtcc
     4021 ggcatcgatc tgcgtgtcgg cgtccagcga cagccgcacc cccttgatca ccgccccggg
     4081 ctgcaggccg gggacgttgg acggctgcag caacgcgcag ccgccgatgc tgacatcgag
     4141 cacgcgcagc gccacgctca tgtcggggat gcccggatgg cggaactgcg ccgtgggcaa
     4201 ggtgcgctcg atggtgcgca cccggaaggt gttgcgccgc tggaagcgga acagctcgcg
     4261 cggcaggcgg gcctgcagca cgctggcctt ggggccctgc accagcacca ggtcgccgac
     4321 gtcgaactgc accttgatgc ggtccaggta ggcgacggcg acgcactcgt cggcctcgac
     4381 gatgtcgtgc accgcgggct gctgcaggtc ggccgtgaac gagacccggg cgttggccgc
     4441 gtcgatcgtc cacagcgtgg tcgtgtaggc gctgccgtcg gaggcgctca ggttcagcgg
     4501 caccacctcg tccatcagct gcttgagcag cgcacggatc tcaccgggcg cgctgacgcg
     4561 gaactcggcg aggccgtcgc cggatcccag cgcgtcgagc gaggcggggc gggtgtcttc
     4621 gaacatggcg ttggtgggtt cgttggggtg ctcgcgaagc gactgatcgg taccagccct
     4681 cgggaagcga gggtcacgcc ccgcgccgat ccgggttccc catcgggggg cagcggccag
     4741 cggagcgtgg gggctcaatg aaggaatgca ggtcggctgc cgccacccat catctggtcg
     4801 aggtcttcca gccagggctc ggccaggtgg cggatctcgg cgtcgttcag gaggatgcgc
     4861 tgcatgatgc gcgacttctc cttggcctcg gtctgcatca gctgcgtcga ttgcgcggcg
     4921 ttcttgagct gcgcgatcag gacggcacag gcgccttcga gcttgaccac ctcgtcccag
     4981 ttgccggcgc gagctgctgc gagcatctcg gcgctggcct tctcgatggc ctcgtagtag
     5041 ttgagcagct tggtactcat gatcttcttc tgggtgaatg tgctcgagat gttcacaggt
     5101 tgcccgcttc cacttgaggt ccgatggcga cccaggcgtc ccgcaggggg cccaggaggc
     5161 gtgcgcactc gtccagcgcg tcctcgtcgt tcctcaggtt ggcctgcagc aggcgcaggc
     5221 cgacgtattg gtacagcgag ctcaggtccg ccgccaggcg gccgccgcct tccatgttca
     5281 ggccggcctt caggccttcc tcgacgatgc gggcggcgcg gccgatggcc ttgcccttgg
     5341 cctcgatctg gcccgcgcgc atggcgccgc gcgccgacac gatggagtcc atgaagccgt
     5401 tgaacagcag ctcgatcagc ttgtgggccg acgcggcgtt gacgctggtc tcgacgccga
     5461 cgcgggcgta cgcgttggca aagccgtagg agcggccgaa tgcggaaggg gtggcggcgg
     5521 agaacatggt gagttcggga tccgtatccg tgaagtgttc gtcttgacct gttatcggaa
     5581 gtgtcggcgc gggtcttgaa gcggaactgt ccggtttgag ccgtcaagtt cgcgcaagca
     5641 gggattcagc ggaacttttc aaggcctccc tacggcggcc gcgggcgccc gaagccgggg
     5701 cgcgtcagct cttgttcatc accgcgatct gctgcgtcac gtagctggag agcgcgttca
     5761 tcttggacat cgtcgtgtcg agcgcctggt actgcgcggt caggcgttcc tgcgtcgcgt
     5821 ccaggtgcgt ctgcatgtcg tcctgctgct gctggttgcg cgagagctcg cccttgaggc
     5881 cggcggtgcg cgagaacagc gagccgtcgg tggcgttggc cttgtcggcg agcgccgcga
     5941 agcgctgggc gatgccggtg ttctcgttgg tcgtgcccca ggtcgagaac agcttggcca
     6001 gctcgtccgg gtgcgccagc gcggaggtca tcttgttcgg gctcgacacg gccagcgtgc
     6061 cgtccgtctg caccgcgatg ccgatgtcgg acaggcgcgc gaacatcgac gaggtcgtcg
     6121 atgtcgtgtt gatgatgccg cgcagctggt tctggaagcc gacgatggac gggtcgccct
     6181 gcagcgggcc gtcctgccgc gacgtgccct gggccaccgc ggtgctggcg tcgtacttgg
     6241 tctgcagcga gatgtaggac tgcagcgccg aatacgccga cacgaagtcg gtgatggcct
     6301 tctgcatgga gcccgtgtcg gccgccaccg tgacgtccac cgggtccgtg gtcttgcgca
     6361 gcagggtcag gctcaggccg tcggagatgt tgctcagctt gttggacgcc gactcgatgt
     6421 cgatgccgtt gaccgtggcc ttggcattca ccgccgactg gttcagcacc atctgcgagt
     6481 tcgtcgcggc ggtgctggcg tcgaagttca gcgccgacag gcccgtgccc gcgacgccgt
     6541 cgtcggtcgt ctcggtggtg gtgacctgga atgcgttggc cgcaccggtg ctggtcgagc
     6601 gcagcgacag gcgcgagccg ttcgcgtcgg tgatgatcga cgcctgcacg ccggcgttgt
     6661 acgagttgat ctggtcgcgg atggccgaca gcgaggtctg gctggccccg atcgggatgt
     6721 cgaccttggg ggagctggcc gcctcgaagg tgaccgtggg cggcgtggcg ccgtcggact
     6781 tgtaggtgcc cagctggatc gagatcgtgc cggcattcag cgtcgagccc gcggcggccg
     6841 gggacgtgac cgtctggccc tgcgccagct gctgcacgct gaccgagtag tcgcccggcg
     6901 cggcggtgcc gttggcggtg tcggccgtca cggcggtcgt gtcggcggag gtggacgtcg
     6961 tctgcgtcca cagcgtcgac gacgacagcg catgcgcctt gtcgctgagc gtggaggtca
     7021 ggctctggat ctggcccacc gccgagatct cggtctgcac cgaggcggcc ttctgctgca
     7081 gcagcgtgag cggctgcgac tccaccgcca tcagcgacgt gatgatgctc ttgacatcca
     7141 gtccgctgcc gatgcccgcg gagctgatgg agccggtcgt cgagctcagg ttggtggtgg
     7201 tcgtcatgca agtccttggc ggttcctagg cggactatcg gcggcccgcc ggcaaacttg
     7261 agggacgagg ccgcgcccga acgcgattgc acgtccggaa acccccgtca attcgaccga
     7321 tcgacggcgc cgccccaatg gaaaggcgcg ccgcgcaaac ctcgggacag ggtcgccggg
     7381 gcttgcgggg cgcgccttcg gggggcgcgg gaaggatcag ccctgcagca gcttgagcac
     7441 ctgctggggc agctggttgg cctgggcgac catcgcggtg ccggcctgct gcagcacctg
     7501 ggcgcggctc atgttggcgg tttccgacgc gaagtcggcg tccatgatcc ggctgcgggc
     7561 ggccgactgg ttctcgacgc cgacctgcag gttggtgatg atcgcgtcga agcggctctg
     7621 cgtggcgccg taggtggcgc gcgtgtcgtt gaccgagtcc agcgccttgt cgatgttgtc
     7681 gatcacggtg ccgatggcgc ccagcgtggc cgaggcgtcg atcagggcgg tcgtggtgtt
     7741 cgtgaccgcg gtgatcgtcg tgtccatcga catgtcggtc gtcgcgatgg tgatcgtgtc
     7801 gtcggcggtg gtgttggcgc cgatctggaa cgtcagcgcg gtcgcgtcgg cgccgaggat
     7861 gtgcttgccg ttgaaggtcg tgccggcgag gacgcggctg atttcctgct gcagctggct
     7921 gaattccttg ttcagcgagt ccttgtcgga cgagctgttg gtggcgttgc gggcctgcac
     7981 cgccagctcg cgcatgcgct gcagcgagtt gctgacctgc gagagcgcgc cttcggcggt
     8041 ctgcgacatc gagatgccgt cgttggcgtt gcggatcgcc acgttcatgc cgcgcgactg
     8101 ggcattcatg cgctcggcga tcgccaggcc ggcggcgtcg tccttggcgg agttcacgcg
     8161 caggccggaa gacaggcgct gcatggacgt cgcgagcgag ccctgcgaca tgttgaggtt
     8221 gcgctgcgca ttcagcgaag caatgttggt attgatcgtc aatggcatgt gaatactcca
     8281 ggactcaggc acaaccggcg aaagtgccgg aactcagttc gatgcgtggc tgtcaggctg
     8341 gccttgcgag ccgcgcacct ccaagcccgg tacttggtca ccccgacttc gcttctcgct
     8401 tcatcgccgg tgcttccgga ccgcgtcccc gacttcccgt gggaggaagg agacgttgga
     8461 ttggattgtg cgaatacttg agtccgggca aaccaccgat atgaggcctc aagttccccc
     8521 aattcctgtc cggggggccc gggatcaccc gagttgtaac cggcgcccgg aatgaagaaa
     8581 gccccgccgg cttgcgcctg gcggggcttg ttttcgaggg gcgggcgggg ctggtcccgc
     8641 ctgctgccgg gcgggtcagc gcagcagcga cagcacttgg gacggctgct ggttggcctg
     8701 ggcgatcatc gcggtgccgg cctgctgcag gacgttggcg cggctcatgt tggccgtttc
     8761 cgtcgcgaag tcggcgtcca tgatgcggct gcgggcggcc gtctggttct cgacgctcga
     8821 ttg
//
LOCUS       DATCRO010000281         8120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_171775, whole genome shotgun sequence.
ACCESSION   DATCRO010000281 DATCRO010000000
VERSION     DATCRO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171775"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VIN75_07700"
     CDS             complement(<1..273)
                     /locus_tag="VIN75_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEY8974086.1"
                     /translation="MSDSPSHPHDARWKHDGVRVVPADKLDPNTAQTPGMDRQAAITF
                     ARTGAQKLWAGTVHIHPNARTGAHHHGPLESVIYVVKGRARMRWGER"
     gene            complement(312..1757)
                     /locus_tag="VIN75_07705"
     CDS             complement(312..1757)
                     /locus_tag="VIN75_07705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8974087.1"
                     /translation="MVAAVQRIERLDRLERIIQTQSLLVQADLALNPFMQLVVDKLRE
                     LTQSHGASVDFVEGRHTVVRRASGAIAGHLGLRVSLDASLSGVCVRTGAVQVCEDSET
                     DPHVDRAACRLLGVRSMICAPLRQGGAVAGVLKVFSDHPRAFDDGDVQMLQLMASAIV
                     AALAKKTAQDARSEAEARLRTSEERMRALLEHAHDAVISIDEHGRVSQWNRAAERLFG
                     WSPIETIGQPVAGLVVPPQMRGEIASVIARYASAERLEDAHQRVTLPAIDRAGQQLSV
                     EVSLTATRVAGRWELTAFAHDVSEKRQLEARLRAMALSDGLTGLANRRGFMEALEKAV
                     SRHARGGPPLALLFLDLDGFKQINDEQGHHVGDLALQTFARRVEACVRKGDTVARLGG
                     DEFTVLAEGIGSLEQARAIADKIIEAMKPPLDTHKAVRLRASIGISLYKPPADASRFL
                     READHAMYHAKHHHDEVEGVSAFMSDEEFLD"
     gene            1875..2321
                     /locus_tag="VIN75_07710"
     CDS             1875..2321
                     /locus_tag="VIN75_07710"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEY8974088.1"
                     /translation="MGVEALSTGPEVQNARRRLTKTLGASSWNRQAARLDPAGGSSMP
                     LAYAACAGGAPIACAARPTFPAQEPRQMEDKTILDRITALVDEEHRLRENPAAPEANA
                     ERLKSVAEQLDQCWDLLRQRRAKREFGADPESAAPRDIGTVENYTN"
     gene            2460..2726
                     /locus_tag="VIN75_07715"
     CDS             2460..2726
                     /locus_tag="VIN75_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974089.1"
                     /translation="MQKNQNQQGQQPSQQDRDSSQSRQQSQSGSQQQQGGHGKLTPSG
                     GSNQQSQSGGGRDQQGSNLKSDQGQAQSRNLQQGSDHTNRSNQR"
     gene            2843..4630
                     /locus_tag="VIN75_07720"
     CDS             2843..4630
                     /locus_tag="VIN75_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974090.1"
                     /translation="MPATAPPSPNPIDAGLYRGIWRHAAGARAAMVGSMLLLMGSQAV
                     RLAVPWFAAQAIDALQKGGPGSLVRALEWVVGVMAASVVSWMLHGPGRVLERRVGMRV
                     RETLSDELYTRLTAAPLAWHDRHHSNDMQQRVRQATQALYEFSANQFIYLQSLVGLVG
                     PLVALVALAPVIGAAAFLAFGALALVSMRIDRTLMRLSMQEMEAERRYGSGMADFLGH
                     IGTVLSLRLQDASRRLLQGRLARVFVPLGRNIVVNEAKWCMVDLVSTLTTWALVVAYV
                     WLLSRDGSGAIAIGAVFMVQQYASRTAAVATDMTGRLQGLSRTRADYASARPIFDAPL
                     AEPVGALPAEWRRIVVRDIAYRHAAAAPDSPPLGLDELALQRGERIALVGGSGAGKST
                     LMRVLAGLYAAPGVVVEVDGRVQPGVHSLAALATLIPQEADVFEGTVRENLDFGTALP
                     EPALAAAMHGSAFDEVMGGLAGGLDFAVAERGSNLSGGQRQRLCLARGALAAAGSSIV
                     FLDEPTGALDPITEARIHRNLDETFPSACIVASVHRMSLLPHFDRVGLMEAGRLVDIG
                     PLAQLRERQPGFARMLEGSAHEADAALGD"
     gene            complement(4732..4875)
                     /locus_tag="VIN75_07725"
     CDS             complement(4732..4875)
                     /locus_tag="VIN75_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974091.1"
                     /translation="MISLNRYRNYIDVVVNPGFAPDLPGQAKGRHGALATAAIGGVAM
                     NLD"
     gene            4967..>8120
                     /locus_tag="VIN75_07730"
     CDS             4967..>8120
                     /locus_tag="VIN75_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEY8974092.1"
                     /translation="MKTSWMTTWRALRHVLAGTVAALALAACGGGGSDSRPPPVTPPV
                     VDPNLADGNSTTLRLVLNAVPSQSASSPTGGTATTLRIHWHRTSGDYTGALIHTWGAA
                     NSPVWNEGFAPDGSDAFGVYFNVQLAQTTGTVGYILHVGDTKDDGGADQSYTLQPGAN
                     EIWRVEGDLTTYTSNPSGAATPDIDTVRVHYLRYANDYSLWGLHLWGGSGLDTSRMGT
                     IAYGDWNNPTPFSAMPNYALGASEATFDIPVLNPTSNAGATSLQFIIHGMPPNTGDKD
                     GRSDNIVVSYANLAIAGRVGQVWLVQGDPTVYASAPDTRSVSTTDQRAVWLTGKLLQW
                     PAVASGSKVKLYWSATGQIVAGLGVPISGADGSITLDDYTGTIPSDVATRFKWVGAGS
                     VYTVRDADVARLATMHTAQVVLVQEDAGGNVQNATTAQIAGAMDDLYAGATSATLGPV
                     VAGGATTYKLWAPTAQSVMLYRYASATGGATNADAMVFDPATGIWSTTVASDTSGSYY
                     RFGVKVFVRGVGLVRNLVTDPYSLTLAADSARSEVVNLDDASTQPAGWTASAPPATVS
                     TEVDQSIYELHVRDFSANDASVPAGHRGKYLAFTDTGSNGMKHLAALAAAGLTDVHLM
                     PMFDFSTVPETGCVTPSPSGAADSQAQQAAVAATQSTDCFNWGYDPYHFNAPEGSYAT
                     SVDDGVTRVKEFRAMVQALNAAGLRVGMDVVFNHTTAAGENDHSVLDEIVPGYYYRLG
                     ANGRQLTDSCCADTAPENHMMGKLVQDSVIQWARDYHVSSFRFDIMSFIPRDVLVALQ
                     AKVDAAAGRHVEMIGEGWNFGTPANGARFVQSSMFSLNGTNIGTFNPYLRDAVRGGSG
                     FDSGNAMVANQGWINGQFYDPNGQGSASAGDLMWSSDVIKAGLAGSVKGYVMNTSWDA
                     SLPLEQINDGGTPLGYAAQPSEAVSYVENHDNQTLFDNNAYKLPATTTQDDRARVQML
                     GAAIVSFSQGVSYFHAGVDTLRSKSLDKNSYDSGDWFNKLDWSYADNNFGVGAPPSGD
                     NSANWSIIKPLLANSSIKPGS"
BASE COUNT         1270 a         2926 c         2795 g         1129 t
ORIGIN      
        1 ccgctcgccc cagcgcatgc gcgcccggcc cttgacgacg tagatcacgc tctccaacgg
       61 cccgtggtgg tgcgcgcccg tcctggcgtt cgggtggatg tgcacggtgc ccgcccacag
      121 cttctgcgcg ccggtgcgcg cgaacgtgat cgcggcctgg cgatccatgc cgggggtctg
      181 ggcggtgttc gggtcgagct tgtcggcggg cacgacgcgc acgccgtcgt gcttccagcg
      241 tgcgtcgtgc ggatgggatg gcgagtcgga cacggcgggc ctttcgcgaa tggcgtcgcc
      301 cgattgtgga ctcagtcgag gaattcctcg tcggacatga acgcggacac gccctcgacc
      361 tcgtcgtggt ggtgcttggc gtggtacatc gcgtggtcgg cctcgcgcag gaagcgcgac
      421 gcgtcggccg gcggcttgta caggctgatg ccgatgctgg cgcgcaggcg caccgccttg
      481 tgcgtgtcca gcggcggctt catcgcctcg atgatcttgt cggcgatcgc gcgcgcctgt
      541 tccagcgagc cgatgccctc ggcgagcacg gtgaactcgt cgccgcccag gcgcgccacg
      601 gtgtcgccct tgcgcacgca ggcctcgacg cggcgcgcga aggtctgcag cgcgaggtcg
      661 ccgacgtggt ggccctgctc gtcgttgatc tgcttgaagc cgtccaggtc gaggaacagc
      721 agcgccaggg gcggtccccc gcgcgcatga cgcgagacgg ccttctcgag cgcctccatg
      781 aagccgcgcc ggttcgcgag gccggtgagg ccgtcgctca gcgccatggc gcgcagccgc
      841 gcctcgagct ggcgcttctc ggagacgtcg tgcgcgaacg cggtcagctc ccagcggccg
      901 gccacgcgcg tggccgtcag gctcacctcc acggacagct gctgccccgc gcgatcgatg
      961 gccggcagcg tgacgcgctg atgggcgtcc tccagccgtt cggcgctggc gtagcgcgcg
     1021 atgacgctgg cgatctcgcc gcgcatctgc ggcgggacga ccaggccggc gaccggctgg
     1081 ccgatcgtct cgatgggcga ccagccgaac agccgttcgg cggcccggtt ccactgcgag
     1141 acgcggccgt gctcgtcgat ggagatgacc gcgtcgtgcg catgctccag cagcgcgcgc
     1201 atgcgctcct cgctggtgcg caggcgcgcc tcggcctcgc tccgggcgtc ctgcgccgtc
     1261 ttcttggcga gcgccgcgac gatggcgctg gccatcagct gcagcatctg cacgtcgccg
     1321 tcgtcgaaag cacgcgggtg gtcggagaag accttgagca ccccggcgac ggcgccgccc
     1381 tggcgcagcg gcgcgcagat catcgagcgc acgcccagca ggcggcaggc ggcgcggtcg
     1441 acgtgcgggt cggtctcgga gtcctcgcag acctgcaccg cgcccgtgcg cacgcacacg
     1501 cccgacaggc tggcgtcgag cgagacgcgc agtccgaggt ggccggcgat ggcgccgctg
     1561 gcgcgccgca cgacggtgtg gcggccttcg acgaagtcga ctgacgcgcc gtgcgactgc
     1621 gtgagctcgc gcagcttgtc gacgacgagc tgcatgaacg ggttcagggc gaggtcggcc
     1681 tggaccagca gagattgcgt ctggatgatg cgttcgagcc gatcgagacg ctcgatccgc
     1741 tgcacagctg ccaccatgga agatccctcg tgcccaagcg cgcgactggc ggccttcgct
     1801 agcgaccgcg tcgcgcgggc gtctgcgaag actcttcttg cgtttggtgc cggaacacca
     1861 aacgcgcagt atgcgtgggc gtcgaagcgc tgtccactgg cccggaagtc caaaacgcgc
     1921 ggcgccgcct gacgaaaacc ctaggtgcgt cgtcatggaa tcgccaggcg gcgcggcttg
     1981 accctgctgg cggcagcagc atgccgctgg cctatgctgc ctgcgctgga ggcgcgccga
     2041 ttgcctgcgc cgcccgaccc acgtttcccg ctcaggagcc acgccaaatg gaagacaaga
     2101 ccattctcga tcgcatcacg gccctcgtcg acgaagagca tcgcctgcgc gagaacccgg
     2161 ccgcgcccga ggccaacgcg gagcgcctca agagcgtcgc ggaacagctc gatcagtgct
     2221 gggacctgct gcgccagcgc cgcgccaagc gcgagttcgg cgccgatccc gagagcgcgg
     2281 cgccgcgcga catcggcacg gtggagaatt acaccaactg agcggttctc ccgatgcgaa
     2341 cgtgaacggg cttcacgaat tgggaatgca ccttgccgcg ctccagggac gacctccgaa
     2401 cttcctcgaa ggccgtctcc accggagaac ggcggggcaa cccaaccaag gagcaacacg
     2461 tgcaaaagaa ccagaaccag caaggccagc aaccgtcgca gcaggatcgc gacagctcgc
     2521 agagccgcca gcagagccag tccggcagcc agcagcagca aggcggccac ggcaagctga
     2581 cgcccagcgg cggttccaac cagcagagcc agtcgggcgg cggacgcgac cagcagggca
     2641 gcaacctgaa gtccgaccag ggccaggcac agagccgcaa cctgcagcaa ggcagcgacc
     2701 acaccaatcg ctcgaaccag cgttgagcga ccgggccgct cggcccgggc atcacgcgac
     2761 agcggccggc ctcgccggcc gtttccgttt gcgtttgcgt ttgccgtatc gtgtccggca
     2821 tcgcaacgcc accgccgccg tcgtgcctgc cactgccccg ccctcgccca acccgatcga
     2881 cgccggtctg taccgcggca tctggcgcca tgccgccggc gcccgtgcgg cgatggtcgg
     2941 ctcgatgctg ctgctgatgg ggtcgcaagc cgtgcgtctg gcagttccct ggttcgcggc
     3001 gcaggcgatc gacgcgctgc agaaaggcgg cccgggcagc ctggtgcgcg cgctcgaatg
     3061 ggtggtcggc gtgatggccg cgtccgtcgt ctcgtggatg ctgcacgggc ccgggcgcgt
     3121 gctcgagcgc cgcgtgggca tgcgcgtgcg cgagacgctg tccgacgagc tctacacgcg
     3181 gctgacggcc gctccgctcg cctggcacga ccgccaccac tccaatgaca tgcagcagcg
     3241 cgtgcgccag gccacgcagg cgctctacga gttctcggcc aaccagttca tctacctgca
     3301 gagcctggtc ggactcgtcg ggccgctcgt cgcgctcgtg gcgctggcgc ccgtcatcgg
     3361 cgccgcggcc ttcctggcgt tcggcgcgct ggcgctcgtc agcatgcgca tcgaccgcac
     3421 cctgatgcgg ctgtcgatgc aggagatgga ggccgagcgc cgctacggct cgggcatggc
     3481 cgacttcctc ggccacatcg gcaccgtgct gagcctgcgg ctgcaggacg cgtcgcggcg
     3541 cctgctgcag ggccggctcg cgcgcgtgtt cgtgccgctg ggtcgcaaca tcgtggtcaa
     3601 cgaggccaag tggtgcatgg tcgacctggt cagcacgctg acgacctggg cgctggtggt
     3661 cgcctatgtc tggttgctga gccgcgacgg ttcgggggcg atcgccatcg gcgcggtctt
     3721 catggtgcag cagtacgcca gccgaaccgc ggcggtcgcc accgacatga ccggccgcct
     3781 gcagggcctg tcgcggacgc gcgcggacta cgccagcgcc cgcccgatct tcgacgcgcc
     3841 cttggccgag ccggtcggcg cgctgcccgc cgagtggcgg cgcatcgtgg tgcgcgacat
     3901 cgcgtaccgc cacgcggccg ccgcgcccga ctcaccgccg ctgggcctgg acgagctcgc
     3961 gctgcagcgc ggcgaacgca tcgcgctggt gggcggcagc ggggccggca agagcaccct
     4021 gatgcgcgtg ctcgccggcc tgtacgcggc gccgggcgtc gtcgtcgagg tcgatggccg
     4081 cgtccagccg ggcgtgcaca gcctggccgc cctcgcgacg ctgatcccgc aggaggccga
     4141 cgtgttcgag ggcacggtgc gcgagaacct ggacttcggc accgccctgc ccgaacccgc
     4201 gctcgccgcc gcgatgcacg gcagcgcctt cgacgaggtg atgggcggcc tggccggcgg
     4261 actcgacttc gccgtggccg agcgcggcag caacctgtcc ggcgggcagc gccagcggct
     4321 gtgcctggca cgcggcgcgc tcgcggcggc gggcagctcg atcgtcttcc tggacgagcc
     4381 caccggtgcg ctcgatccga tcaccgaggc ccgcatccac cgcaacctcg acgaaacctt
     4441 tccgtcggcc tgcatcgtgg catcggtgca ccgcatgagc ctgctgccgc atttcgaccg
     4501 cgtcgggttg atggaggccg gacgcctggt cgacatcggc ccgctcgcgc agctgcgcga
     4561 acgccagccc ggcttcgcgc gcatgctcga gggcagcgcg cacgaggccg acgcggcgct
     4621 cggcgactga tcggccgcgg aagatcgcgc gcagcagcag gacgtacccg gcaaccgcca
     4681 gcacggcgtg tccgatcgtc agcccgatcg gcagcggcag ccccgccagt gctagtcgag
     4741 gttcatcgcg acgccgccga tggccgcggt cgcaagcgca ccgtgcctgc ccttcgcctg
     4801 gcccggcagg tccggcgcga agcctgggtt gactacaaca tcgatgtagt tacggtaacg
     4861 gttcagtgaa atcaccattt gtcgatgtag tttgacttcc ttacgatgtc gccaagccga
     4921 gcagcgttcc tggagcagcg cgccccggca ccacaacgga gacacgatga agacaagttg
     4981 gatgacaaca tggcgcgcgc tgcgtcacgt cctggccggc accgtcgcgg cgctcgcgct
     5041 cgcggcctgc ggtggcggag gcagcgacag ccggccgccg ccggtgactc cccccgtcgt
     5101 cgatcccaac ctggcggacg gcaattccac caccctgcgc ctcgtgctga acgcggtgcc
     5161 gtcccagtcc gcctcgtccc cgaccggcgg cacggcgacg acgctgcgga tccactggca
     5221 ccgcacgagc ggcgactaca ccggcgcgct gatccacacc tggggcgccg cgaactcgcc
     5281 ggtctggaac gagggcttcg ccccggacgg cagcgacgcc ttcggcgtgt acttcaacgt
     5341 gcagctggcc cagaccaccg gcacggtcgg ctacatcctg cacgtgggcg acaccaagga
     5401 tgacggcggc gccgaccagt cctacacgct gcagcccggc gccaacgaga tctggcgcgt
     5461 cgagggcgac ctgacgacgt acaccagcaa tccgtccggc gccgccacgc ccgacatcga
     5521 caccgtgcgc gtgcactacc tgcgctacgc caacgactac tcgctgtggg gcctgcacct
     5581 gtggggcggc agcgggctgg acacgtcccg catgggcacc atcgcctacg gcgactggaa
     5641 caacccgacg ccgttcagcg cgatgcccaa ctacgcgctc ggcgcgtccg aggccacctt
     5701 cgacatcccg gtgctgaacc cgacgagcaa cgccggcgcg acgtcgctgc agttcatcat
     5761 ccacggcatg ccgcccaaca ccggcgacaa ggatggccgc agcgacaaca tcgtcgtcag
     5821 ctacgcgaac ctggccatcg ccgggcgcgt cgggcaggtg tggctggtgc agggcgaccc
     5881 caccgtctac gccagcgcgc ccgacacgcg ctcggtctcc acgaccgacc agcgcgccgt
     5941 gtggctgacg ggcaagctgc tgcagtggcc ggcggtcgcc agcggaagca aggtcaagct
     6001 gtactggtcg gccaccggcc agatcgtcgc cggactgggc gtgcccatca gcggcgccga
     6061 cggctcgatc acgctggacg actacaccgg caccatcccg tccgacgtcg ccacgcgctt
     6121 caagtgggtc ggcgcgggca gcgtgtacac ggtgcgcgac gccgatgtcg cgcgcctggc
     6181 caccatgcac acggcgcagg tcgtcctcgt tcaggaggat gccggcggca acgtgcagaa
     6241 cgcgaccacc gcgcagatcg ccggcgcgat ggacgacctg tacgccggcg ccacgagcgc
     6301 cacgctgggc ccggtcgtgg ccggcggcgc caccacgtac aagctgtggg cgccgacggc
     6361 gcagagcgtc atgctgtacc gctacgcgtc ggccacgggc ggcgcgacga acgcggacgc
     6421 catggtgttc gacccggcga ccggcatctg gagcaccacg gtcgcctccg acaccagcgg
     6481 aagctactac cgcttcggcg tcaaggtgtt cgtgcgcggc gtcgggctcg tgcgcaacct
     6541 cgtcaccgat ccctactcgc tgacgctggc cgccgacagc gcgcgcagcg aggtcgtcaa
     6601 cctcgacgac gcaagcacgc agcccgccgg ctggaccgcg agcgccccgc cggcgacggt
     6661 gtccaccgag gtcgaccagt cgatctacga gctgcacgtg cgcgacttct cggccaacga
     6721 cgcgagcgtg ccggccggac accgcggcaa gtacctggcg ttcaccgaca ccggctccaa
     6781 cggcatgaag catctggccg cgctggccgc cgccggcctg accgacgtgc acctgatgcc
     6841 catgttcgac ttctccaccg tgccggagac cggctgcgtc acgcccagcc cgagcggcgc
     6901 ggcggacagc caggcccagc aggcggccgt cgctgcgacg caatcgaccg actgcttcaa
     6961 ctggggctac gacccgtacc acttcaacgc gcccgaaggc agctacgcca cctcggtgga
     7021 cgacggcgtc acgcgcgtca aggagttccg cgcgatggtc caggcgctca acgccgcggg
     7081 cctgcgcgtg ggcatggacg tcgtgttcaa ccacaccacc gcggcgggcg agaacgacca
     7141 ttcggtactc gacgagatcg tgccgggcta ctactaccgc ctgggcgcga acggccggca
     7201 gctgaccgat tcgtgctgcg ccgacaccgc gcccgagaac cacatgatgg gcaagctggt
     7261 gcaggactcg gtgatccagt gggcgcgcga ctaccacgtc agctcgttcc gcttcgacat
     7321 catgagcttc atcccgcgcg acgtcctcgt ggcgctgcag gccaaggtgg atgcggccgc
     7381 cgggcggcac gtggagatga tcggcgaagg ctggaacttc ggcacgccgg ccaacggcgc
     7441 gcgcttcgtg cagtccagca tgttcagcct caacggcacg aacatcggca cgttcaaccc
     7501 gtacctgcgc gacgccgtgc gcggcggcag cggcttcgac agcggcaacg cgatggtcgc
     7561 caaccagggc tggatcaacg gccagttcta cgacccgaac ggccagggca gcgccagcgc
     7621 cggcgacctg atgtggagca gcgacgtcat caaggcgggc ctggccggct ccgtcaaggg
     7681 ttacgtcatg aacaccagct gggacgcgag cctgccgctg gagcagatca acgacggcgg
     7741 cacgccgctg ggctacgcgg cgcagccgtc ggaggcggtc agctacgtcg agaaccacga
     7801 caaccagacc ctgttcgaca acaacgccta caagctgccg gccacgacga cacaggacga
     7861 ccgcgcccgc gtgcagatgc tgggggcggc catcgtgtcg ttcagccagg gcgtgtcgta
     7921 cttccacgcg ggcgtggaca cgctgcgcag caagtcgctg gacaagaaca gctacgactc
     7981 gggcgactgg ttcaacaagc tggactggag ctacgccgac aacaacttcg gcgtgggcgc
     8041 gccgccctcg ggcgacaaca gcgcgaactg gtcgatcatc aagccgctcc tggccaacag
     8101 ctcgatcaag ccgggctcgg
//
LOCUS       DATCRO010000282         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_734492, whole genome shotgun sequence.
ACCESSION   DATCRO010000282 DATCRO010000000
VERSION     DATCRO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734492"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VIN75_07735"
     CDS             <1..1213
                     /locus_tag="VIN75_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8974093.1"
                     /translation="WAWAVIARHGAQSLMPEAVDWADRAWAARKPARKGARLGPPDWS
                     DDVLAWHARAALRAGEGDKRWKDVERAIDAMSERERAMDNWVYWRAQAELALAIGMPA
                     AESDATVAAARAQLKALSSSVSFYGLLASEDLGVPFALPPKPIPVTPLERGLARANPG
                     LQRGLALTALELRDEGRREWNYTLRGMNDRDMLAAAQWACDMSDWQLCINTSERTKVE
                     IDVAQRYPMPFRRDIVEAATNAGLDPAFVFGLIRQETRFMGNMRSAVGANGLMQLMPA
                     TAKWAARKANIPYRPDLITDATTNLRIGSFYLKMVLESFGGSDPMAAAAYNAGPARPR
                     RWRGGLMLAPEVWTENVPFYETRDYVKKVMTNAAIYAALLGNKGPQLRPRLGPQIGPR
                     DAEQPPAPDMP"
     gene            1210..2442
                     /locus_tag="VIN75_07740"
     CDS             1210..2442
                     /locus_tag="VIN75_07740"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY8974094.1"
                     /translation="MKAFVVGGAVRDGLLGEPVNDRDWVVTGASPQDMLDAGYLPVGK
                     DFPVFLHPQTHEEYALARTERKTGPGYRGFEVYAAPDVTLEDDLRRRDLTINAIAQQP
                     DGTLVDPHHGQRDLQARVLRHVSEAFTEDPVRILRLARFAARWPEFTVAPETDALMKQ
                     MVAAGEVDALVAERVWQELSRGLMERAPARMFEVLRGVGALARLLPEVDALFGVPQPP
                     QHHPEIDTGVHTLMVLDRCAATAQPLAVRYACLVHDLGKGTTPADILPRHIGHEQRGE
                     DLIRGVNERLRVPTDCGELALLTAREHSNVHGSGKLDATAVMRLLERCDALRRPERFL
                     QMLAACECDATGRLGLEDLPYPQRARLAAALKIAQDVDARAASTRAIERGLKGEAIAA
                     EIRYARVLALKAGLDAAD"
     gene            2535..3080
                     /locus_tag="VIN75_07745"
     CDS             2535..3080
                     /locus_tag="VIN75_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8974095.1"
                     /translation="MKLSARDTALVVIDMQAILFDPEPAEAEEVVARINGLASRARAA
                     RVPVVWVQHETATGDLVADTPGWRLHPGLVADAADLFLRKTMSDAFLRTPLHERLAER
                     GVRHVVVCGYSSEFCVDSSVRGAASRGYAVTLAADAHTSHDKPHATGLQIRLHENETL
                     RNISSFHVVIDAVPAAEITFG"
     gene            complement(3105..3464)
                     /locus_tag="VIN75_07750"
     CDS             complement(3105..3464)
                     /locus_tag="VIN75_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8974096.1"
                     /translation="MLDWLAYRRELTGRIGQFAKLSPGTVQGYQALSKAGAATGHLDA
                     KTRELIALAVSVTTRCDGCITVHTAEALRQGASKDEIAEALGVAVALNAGAAMVYSAR
                     TLDAVDAYAAGAATPAA"
     gene            complement(3792..3899)
                     /locus_tag="VIN75_07755"
     CDS             complement(3792..3899)
                     /locus_tag="VIN75_07755"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HEY8974097.1"
                     /translation="MPGDIRLRIAGRELFVPLGSGVALFLVASAIAWIR"
BASE COUNT          555 a         1459 c         1384 g          505 t
ORIGIN      
        1 ctgggcgtgg gccgtcatcg cccggcatgg cgcgcagtcg ctgatgcccg aggccgtcga
       61 ctgggccgac cgcgcctggg cggcgcgcaa gccggcgcgc aagggcgcca ggcttggccc
      121 gcccgactgg agcgacgacg tgctcgcgtg gcacgcgcgc gccgcgctgc gcgcgggcga
      181 gggcgacaag cgctggaagg atgtcgagcg cgcgatcgac gcgatgagcg aacgcgagcg
      241 cgcgatggac aactgggtct actggcgcgc gcaggcggag ctcgcgctgg cgatcggcat
      301 gccggccgcc gagagcgacg ccaccgtggc cgccgcgcgc gcgcagctca aggcgctgtc
      361 gtccagcgtg agcttctacg gcctgctcgc ctcggaggat ctcggcgtgc cgttcgcgtt
      421 gccgcccaag cccatcccgg tcacgccgct ggagcgcggg ctggcgcgcg ccaacccggg
      481 cctgcagcgc ggcctggcgc tcacggcgct cgaactgcgc gacgaggggc gccgcgagtg
      541 gaactacacg ctgcgcggca tgaacgaccg cgacatgctc gccgccgcgc agtgggcctg
      601 cgacatgagc gactggcagc tgtgcatcaa caccagcgag cggaccaagg tcgagatcga
      661 cgtcgcgcag cgctacccga tgccgttccg gcgcgacatc gtcgaggccg ccaccaacgc
      721 gggactcgac ccggccttcg tgttcggcct gatccgccag gagacgcgct tcatgggcaa
      781 catgcgctcg gccgtcggcg ccaacggcct gatgcagctg atgccggcca ccgccaagtg
      841 ggccgccagg aaggccaaca tcccgtaccg gcccgacctc atcaccgacg ccaccaccaa
      901 cctgcgcatc ggcagcttct acctcaagat ggtgctggag agcttcggcg gctccgatcc
      961 gatggccgcg gccgcctaca acgccggccc cgcgcgcccg cggcgctggc gcggcgggct
     1021 catgctcgca cccgaggtct ggacggagaa tgtgccgttc tacgagaccc gggactacgt
     1081 caagaaggtg atgaccaacg ccgccatcta cgccgcgctg ctgggcaaca agggcccgca
     1141 gctgcgcccc cgcctcggcc cgcagatcgg cccgcgcgac gccgagcagc cgccggcgcc
     1201 ggacatgcca tgaaggcctt cgttgtcggc ggcgcggtgc gcgacggcct gctcggcgag
     1261 ccggtcaacg accgcgactg ggtcgtcacc ggcgcgagcc cgcaggacat gctggacgcc
     1321 ggctacctgc cggttggcaa ggacttcccg gtcttcctgc acccgcagac gcacgaggaa
     1381 tacgcgctgg cgcgcaccga gcgcaagacc gggcccggct accgcggctt cgaggtctac
     1441 gcggcgcccg acgtcacgct ggaggacgac ctgcgccggc gcgacctcac gatcaacgcg
     1501 atcgcgcagc agcccgacgg cacgctggtc gacccgcacc acggccagcg cgacctgcag
     1561 gccagggtgc tgcgccacgt ctccgaggcg ttcaccgagg acccggtgcg catcctgcgc
     1621 ctggcacgct tcgcggcgcg ctggccggag ttcacagtcg cgcccgagac ggacgccttg
     1681 atgaagcaga tggtcgcggc cggcgaggtc gacgcgctgg tggccgagcg cgtgtggcag
     1741 gagctgtcgc gcggcctgat ggagcgcgcg ccggcccgca tgttcgaggt gctgcgcggg
     1801 gtcggcgcgc tcgcgcgcct gctccccgag gtcgacgcgc tgttcggcgt gccgcagccg
     1861 ccgcagcacc atccggagat cgacaccggc gtgcacacgc tgatggtgct cgatcgctgc
     1921 gccgccaccg cgcagccgct ggcggtgcgc tacgcctgcc tggtgcacga cctgggcaag
     1981 ggcacgacgc ccgccgacat cctgccgcgc cacatcggcc atgagcagcg cggcgaggac
     2041 ctgatccgcg gcgtcaacga gcgcctgcgg gtgcccaccg actgcggcga gctcgcgctg
     2101 ctgacggcac gcgagcacag caacgtccac ggcagcggca agctcgacgc cacggccgtc
     2161 atgcgcctgc tcgaacgctg cgacgcgctg cgccgccccg agcgcttcct gcagatgctg
     2221 gccgcctgcg agtgcgatgc caccggccgc ctgggcctgg aggacctgcc gtacccgcag
     2281 cgcgcgcgcc tggccgcggc cctgaagatc gcgcaggacg tcgatgcccg cgccgcgtcg
     2341 acgcgcgcca tcgaacgcgg actgaaaggc gaggcgatcg ccgccgagat ccggtacgcg
     2401 cgtgtcctcg cgctcaaggc cggcctggac gccgccgatt gatccgcgcc gcgcagcagt
     2461 gtcgtgcccg cgccggcgtt tacaccgcgc gccggacggc cgatgatgcg gctccccgag
     2521 accgcccttc ctccctgaag ctttccgccc gcgacaccgc cctcgtcgtc atcgacatgc
     2581 aggcgatcct gttcgacccc gagccggccg aggccgagga ggtcgtcgcc cgcatcaacg
     2641 gcctcgcgtc ccgcgcgcgc gccgcgcgcg tgccggtcgt ctgggtgcag cacgagacgg
     2701 ccacgggcga cctcgtggcc gacacgccgg gctggcggct gcatccgggc ctggtcgccg
     2761 acgcggccga cctgttcctg cgcaagacga tgtccgatgc cttcctgcgc acgccgctgc
     2821 acgagcgcct ggccgagcgc ggcgtgcgcc acgtcgtcgt gtgcggctat tcgagcgagt
     2881 tctgcgtcga ctccagcgtc cgcggcgccg cctcgcgcgg ctacgcggtg acgctcgccg
     2941 ccgatgccca cacctcgcat gacaagccgc acgccacggg cctgcagatc cggctgcacg
     3001 agaacgagac gctgcgcaac atcagcagct tccacgtcgt gatcgacgcg gtgccggcgg
     3061 ccgagatcac gttcgggtga acgcgatccc tttctcgcgg agggtcaggc tgccggcgtg
     3121 gcggcgcccg cggcgtatgc gtcgaccgca tccagcgtcc gcgccgaata gaccatcgcg
     3181 gcgccggcgt tgagcgccac ggccacgccg agcgcctcgg cgatctcgtc cttgctcgcg
     3241 ccctgcctga gcgcctcggc ggtgtgcacc gtgatgcagc cgtcgcagcg cgtggtcacc
     3301 gacacggcca gcgcgatcag ctcgcgcgtc ttggcgtcga ggtgcccggt ggccgccccg
     3361 gccttggaca gcgcctggta gccctgcacg gtgcccggcg acagcttggc gaattggccg
     3421 atgcggccgg tgagttccct gcgataggcg agccagtcca gcatgtcgtt tctcctgggt
     3481 tcgtgacgcc ggggatgtcc ggcgccgacg tcgccagtgt gaaccgatgg ggcgagacct
     3541 tccatgccgg aacgtcgcgg tggattgctc ggacgtctcc accctccacg cggcgtcatc
     3601 ccgcgcgcag cgaagcggag tcgcaggatg acactttggt gggcgcgagg cggcaacgtc
     3661 gcacggcgtc gggacgacga tgccgctcat cgttcgcgga gcggcatgga caggttggcc
     3721 gcaggcccga cccgccgcgt cggcacatcg gaacgccggc gaacggcggg ttacgcctcc
     3781 ggctgacccc cctaccggat ccaggcgatc gcgctcgcga cgaggaacaa cgccacgccg
     3841 ctgcccagcg gcacgaacag ctcgcggcca gcgatgcgca ggcggatgtc gcccggcagg
     3901 cgg
//
LOCUS       DATCRO010000283         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_925672, whole genome shotgun sequence.
ACCESSION   DATCRO010000283 DATCRO010000000
VERSION     DATCRO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925672"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..694
                     /locus_tag="VIN75_07760"
     CDS             44..694
                     /locus_tag="VIN75_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8974098.1"
                     /translation="MKRRHFSALAASVATAALPRLAVAQDDAPAIARPRDWTAIAPPV
                     PVDVPAGQVDVVEFFSFACPHCFAFERPLQAWLARKPPQVRFRRSPVPFQRNFRNFQP
                     MWFALEALGLADTLARATFDAVHRDGLRLDTPADIAAFMARHGVDPDRFMRLFDAPDT
                     AAQVAQADRLFDASGAGSVPTLMVQGRFLTSPWTARGGARALEVVDALVARIRADG"
     gene            838..1476
                     /locus_tag="VIN75_07765"
     CDS             838..1476
                     /locus_tag="VIN75_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8974099.1"
                     /translation="MQRRHFSTLAAASLGLGLTRLANAQGPITSQDYKPVAMPVVMTA
                     PAGQVDVVEFFSYACPHCNDFEPVLEAWLRKKPAGIHFHRYPVRFLQNAANFQPMYFA
                     LEAMDQVDAMQQKIFDAVHKEGQRLDKPDAIAAFMARNGLDAKRFMSLFTSFSVRTKV
                     QQANQMMETFGIDSVPTIVVDGKWTTSPADAKGEERTTQVVEYLAAKARGGH"
     gene            complement(1521..2285)
                     /locus_tag="VIN75_07770"
     CDS             complement(1521..2285)
                     /locus_tag="VIN75_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8974100.1"
                     /translation="MTKALSVVRSILFLAYMTITVIPWGFVVVVCSPFLSSDAVYSWC
                     INWVKLAHNGSRWICGVSKRVIGMENLPTAEQATAAVILASKHQSTWETFALPAVMPH
                     PLAYVFKKELLYIPFFGWAMSRLDMIHIDRSKRTEAWNKVAEQGKRLSKLGKWVIMFP
                     EGTRTPRGSQGNYKAGATRLAIAVGVPVVPIAVDSARCWPRKSFWLKPGVVTISIGKP
                     ISPQGREPDEMMREVEAWIEAEMRRLDPEAYAGTPA"
     gene            complement(2310..2876)
                     /gene="gmhB"
                     /locus_tag="VIN75_07775"
     CDS             complement(2310..2876)
                     /gene="gmhB"
                     /locus_tag="VIN75_07775"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY8974101.1"
                     /translation="MPMQAPKLVITGRDGILNVFRSDHVKAPEEWQPIPGALEAVARL
                     NHAGWHVVVATNQSGIGRGMIDMTSVNAVHQHMMKLLAEKGGRIDAVFFCPHAATEQC
                     ECRKPAPGLMHDIGERYGVELHTVPMVCDTLRDLEAARAAGCQPHLVRSGRAAEADDA
                     TIAAWMQAVPGTVVHDDLSAFAEHVTGN"
     gene            complement(3042..>3518)
                     /locus_tag="VIN75_07780"
     CDS             complement(3042..>3518)
                     /locus_tag="VIN75_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY8974102.1"
                     /translation="AGSLREQGYSAHEVDAVLALGVQRLADIPRRLEAVRAFAALPEA
                     AALAAANKRIANILKKSADPVEAKADAALFSEDAERALWADVATVRRSAEVLFEAGDY
                     TSSLKALAALRAPVDRFFDDVMVNADDPKVRANRLGLLATLHGVMNRVADLSRLTT"
BASE COUNT          485 a         1295 c         1202 g          536 t
ORIGIN      
        1 atgcggcgcg gccccgccgc caaccccctg cacaggattc cgcttgaagc gtcgccattt
       61 ctccgccctc gccgccagcg tcgccaccgc cgccttgccc cgcctggccg tcgcgcagga
      121 cgacgcgccc gccatcgccc ggccgcgcga ctggacggcc atcgccccgc cggtgccggt
      181 cgacgtcccc gcggggcagg tggacgtcgt cgagttcttc agcttcgcct gcccgcactg
      241 cttcgccttc gagcgcccgc tgcaggcctg gctcgctcgc aagccgccgc aggtccgctt
      301 ccgccgctcg cccgtgccgt tccagcgcaa cttccgcaac ttccagccga tgtggttcgc
      361 gctggaggcg ctggggctgg ccgacaccct cgcgcgcgcg accttcgacg ccgtgcatcg
      421 ggacggcctg cgcctggaca cgccggccga catcgccgcg ttcatggcgc gccatggcgt
      481 cgatcccgac cggttcatgc gcttgttcga cgcccccgac accgccgcgc aggtcgcgca
      541 ggccgaccgg ctgttcgatg ccagcggcgc cggcagcgtg cccacgctga tggtgcaggg
      601 ccgcttcctc acctcgccct ggacggcgcg cggcggcgcc cgggcgctgg aggtggtcga
      661 cgccctggtt gcgcggatcc gggccgacgg ctgaccgccc cggctcgacg gcaggacgcg
      721 cgcggccggg aacctccgcc gtgcagccgg aaccaagagt ggtcacaatg cccccttcct
      781 ggcgccggcg cttccgcgat ggagcgcccg cgccttcagc cccctgaaag gattcgcttg
      841 caacgccgcc acttctccac gctcgccgcc gcctcgctcg gcctgggcct gacccgactc
      901 gcgaacgccc agggcccgat caccagccag gactacaagc ccgtggccat gccggtcgtc
      961 atgaccgcgc ccgccggcca ggtcgacgtc gtcgagttct tcagctatgc ctgcccccat
     1021 tgcaacgact tcgagcccgt gctcgaggcc tggctgcgca agaagccggc cggcatccat
     1081 ttccaccgct atccggtgcg cttcctgcag aacgccgcca acttccagcc gatgtacttc
     1141 gcgctcgagg ccatggacca ggtcgacgcg atgcagcaga agatcttcga cgccgtgcac
     1201 aaggaaggcc agcgcctgga caagcccgac gccatcgccg cgttcatggc caggaacggc
     1261 ctggacgcca agcgcttcat gtcgctgttc acgtcgttca gcgtgcgcac caaggtgcag
     1321 caggccaacc agatgatgga gaccttcggc atcgactcgg tgcccaccat cgtcgtcgac
     1381 ggcaagtgga ccacctcgcc cgccgacgcg aagggcgagg agcgcacgac gcaggtcgtc
     1441 gagtacctgg ccgccaaggc gcgcggcggc cactgagcct cccgtgcgag cgcgcgccgg
     1501 ccgaccggcc cgcgcgggcg tcaggccggc gtgccggcgt aggcctcggg gtcgagccgg
     1561 cgcatctcgg cctcgatcca cgcttcgacc tcgcgcatca tctcgtcggg ctcgcggccc
     1621 tgcggcgaga tcggcttgcc gatggagatg gtcacgacgc cgggcttgag ccagaagctc
     1681 ttgcgcggcc agcagcgcgc cgaatcgacg gcgatcggca ccaccggcac gcccaccgcg
     1741 atggccaggc gcgtggcgcc ggccttgtag ttgccctggc tgccgcgcgg cgtgcgcgtt
     1801 ccctccggga acatgatcac ccacttgccc agcttgctca ggcgcttgcc ctgctcggcc
     1861 accttgttcc aggcctcggt tcgcttgctg cggtcgatgt ggatcatgtc cagccgcgac
     1921 atcgcccagc cgaagaacgg gatgtagagc agttccttct tgaagacgta ggccagcggg
     1981 tgcggcatca ccgccggcag cgcgaacgtc tcccaggtgg actggtgctt ggaagccagg
     2041 atcaccgccg cggtggcctg ttcggccgtc ggcaggttct ccatgccgat cacgcgcttg
     2101 ctcacgccgc agatccagcg cgagccgttg tgcgccagct tcacccagtt gatgcaccag
     2161 gagtagacgg cgtcgctgct gaggaacggc gaacagacga ccaccacgaa gccccacggg
     2221 atcaccgtga tcgtcatgta ggccaggaac aggattgaac ggacgacgga gagggccttg
     2281 gtcatgcctt gatgttgttc tcgaggattt cagttgccgg tgacgtgctc ggcgaacgcc
     2341 gacaggtcgt cgtgcaccac ggtgcccggc acggcctgca tccacgcggc gatggtggcg
     2401 tcgtcggcct cggcggcgcg gccgctgcgc accaggtgcg gctggcagcc ggcggcccgg
     2461 gcggcctcga ggtcgcgcag cgtgtcgcag accatgggga cggtgtggag ctccacgccg
     2521 tagcgctcgc cgatgtcgtg catcaggccg ggcgcgggct tgcggcactc gcactgctcg
     2581 gtggccgcat gcgggcagaa gaacacggcg tcgatgcgcc cgcccttctc cgccagcagc
     2641 ttcatcatgt gctggtgcac ggcgttcacc gaagtcatgt cgatcatgcc gcggccgatg
     2701 cccgactggt tggtcgccac caccacgtgc cagcccgcat ggttcagccg cgcgacggcc
     2761 tccagcgcgc cggggatcgg ctgccactcc tccggcgcct tcacgtggtc ggagcggaag
     2821 acgttgagga tgccgtcgcg accggtgatc acgagcttgg gggcttgcat gggcatgtcg
     2881 gggcctttag tcgcagtgaa ctcgaagctg cgcatccccg gccggcgagc gctcggccgc
     2941 tccggagaga gccggccttc cgtgaggcgt tcgccagtcc actgggctgg cgcacgtccg
     3001 cacggccccc tcgggggcgg ggagcggagc gagccgggtc gtcatgtcgt caggcgcgag
     3061 aggtccgcga cgcggttcat cacgccgtgc agcgtggcca gcaggcccag ccggttggcg
     3121 cgcaccttcg ggtcgtcggc gttgaccatc acgtcgtcga agaacctgtc gaccggcgcg
     3181 cgcagcgcgg ccagcgcctt caggctgctg gtgtagtcgc cggcctcgaa cagcacttcg
     3241 gccgagcggc gcaccgtggc gacgtcggcc cacagcgcgc gctcggcgtc ctcggagaac
     3301 agcgccgcgt cggccttggc ctcgaccgga tcggcgctct tcttgaggat gttggcgatg
     3361 cgcttgttgg cggcggccag cgcggcggcc tcgggcagcg cggcgaacgc gcgcacggcc
     3421 tcgaggcggc gcgggatgtc ggccaggcgc tgcacgccga gcgccagcac ggcgtcgacc
     3481 tcgtgcgccg agtagccctg ctcgcgcagg ctgcccgc
//
LOCUS       DATCRO010000284         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_25445, whole genome shotgun sequence.
ACCESSION   DATCRO010000284 DATCRO010000000
VERSION     DATCRO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25445"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2159
                     /locus_tag="VIN75_07785"
     CDS             <1..2159
                     /locus_tag="VIN75_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974103.1"
                     /translation="LITITLQLTAPGTANADPATHTASLFLFLLSVSSLTLIVNVVVQ
                     QQRRLAGELAFRAQQERVELMLQAATDAVISFDEGGALSYWNPAAERLFGRSAADALG
                     RPVTQILPLPKLVAATDGIAALCDANDELFSGAVTEFDALDAAGRRLPVELSLTAYRN
                     DRGWAATAFMRDARTHKRQEEALRLARDKAEEATRAKSLFLATMSHELRTPLSGIIGM
                     LQLGLREAMPAQSRSRVSLALGNAEALLAIINDILDFSKIEAGKMGFESVDFDLREMV
                     QSLVELLDLRAQEEGLSLLSHVDPNVPRWLRGDPMRLRQILLNLIGNALKFTEVGTVE
                     LRVACGATETGRLLLEIEVEDTGIGISHEAQQRLFSSFEQADVTTTRKYGGTGLGLTI
                     TRALVEGMAGEITLRSAVGVGSTFRVQLPVALGSPVETVAEEALPAFDCRLNVLCAED
                     GHTNRVIVQAFVQGLGHDVSFVENGLQAVQRCAEENFDVILMDGRMPVMDGLAAARAI
                     RAGGIGETWVLDPAIRMIALTANATGNDRELCLAAGMDDFLSKPVDERLLAQALHRTV
                     AALRARGRELRPLLDTMAMAGGSTAALDALFLDNEPDAASVDAAEAVLADAAAKPPPS
                     APAVRSLSERLADAFRTETPRLLADLADAITRRDFGRAARIAHNIKGSAFYIHSDPMA
                     DDAGGLERACDIGDDAAVERHWAALQESIDDWMTAH"
BASE COUNT          313 a          791 c          766 g          297 t
ORIGIN      
        1 gcctgatcac catcacgctg cagctcaccg cgccgggcac cgccaacgcc gatccggcca
       61 cgcacaccgc ctcgctgttc ctgttcctgc tgagcgtgtc gtcgctgacg ctgatcgtca
      121 acgtcgtcgt gcagcagcag cgccggctgg ccggcgagct ggccttccgg gcgcagcagg
      181 agcgcgtcga gctgatgctc caggccgcca ccgacgccgt catcagcttc gacgagggcg
      241 gggcgctgtc gtactggaat ccggcggccg agcggttgtt cgggcgcagc gccgccgacg
      301 cgctgggccg gccggtgacg cagatcctgc cgttgcccaa gctggtggcc gccaccgacg
      361 gcatcgccgc gctgtgcgac gccaacgacg agctgttctc cggcgccgtc accgagttcg
      421 atgcgctgga cgctgccggc cggcgcctgc cggtggagct gtcgctgacg gcctaccgca
      481 acgatcgcgg ctgggccgcc acggccttca tgcgcgacgc gcgcacgcac aagcgccagg
      541 aagaggcgct gcgcctggcg cgcgacaagg ccgaggaggc cacgcgcgcg aagtcgctgt
      601 tcctcgccac gatgagccac gagctgcgca cgccgctgtc gggcatcatc ggcatgctgc
      661 agctgggcct gcgcgaggcg atgccggcgc agtcccgctc gcgcgtgtcg ctggcgctgg
      721 gcaacgccga ggcgctgctg gccatcatca acgacatcct cgacttctcg aagatcgagg
      781 ccggcaagat gggcttcgag tcggtcgact tcgacctgcg cgagatggtg cagtcgctgg
      841 tcgagctgct cgacctgcgc gcccaggagg aaggcctgtc gctgctgagc catgtcgacc
      901 cgaacgtgcc gcggtggctg cgcggcgacc cgatgcggtt gcgccagatc ctgctcaacc
      961 tgatcggcaa cgcgctcaag ttcaccgagg tcggcaccgt ggagctgcgc gtcgcctgcg
     1021 gagccaccga gacgggccgg ctgctgctgg agatcgaggt cgaggacacc ggcatcggca
     1081 tctcgcacga ggcgcagcag cgcctgttca gcagcttcga gcaggcggac gtcaccacca
     1141 cgcgcaagta cggcggcacc ggcctgggcc tgacgatcac gcgcgcgctg gtcgagggca
     1201 tggccggcga gatcaccttg cgcagcgcgg tgggcgtggg cagcacgttc cgcgtgcagt
     1261 tgccggtggc gctcggctcg ccggtcgaga cggtggccga ggaggccctg cccgcgttcg
     1321 actgccgcct gaacgtgctg tgcgcggagg acggccacac caaccgcgtg atcgtgcagg
     1381 ccttcgtgca gggcctggga cacgacgtca gcttcgtcga gaacggcctg caggcggtgc
     1441 agcgctgcgc cgaggagaac ttcgacgtca tcctcatgga cggccgcatg ccggtcatgg
     1501 acggcctcgc ggccgcacgg gcgatccgcg ccggcggcat cggcgagacc tgggtgctgg
     1561 atccggccat ccgcatgatc gcgctcaccg ccaacgccac cggcaacgac cgcgagctgt
     1621 gcctggccgc gggcatggac gacttcctgt ccaagccggt cgacgaacgc ctgctcgcgc
     1681 aggcgctgca tcgcaccgtc gccgcgctgc gcgcgcgcgg ccgcgagctg cgcccgctgc
     1741 tggacacgat ggcgatggcc ggcgggtcga ccgcggcgct cgacgcgctg ttcctcgaca
     1801 acgagcccga cgccgcgtcc gtcgacgccg ccgaggccgt gctggccgat gccgcggcca
     1861 agccgccgcc atcggcgccc gcggtgcgct cgctgtccga gcgcctggcc gacgcgttcc
     1921 gcaccgagac gccgcgcctc ctggccgacc tcgccgacgc gatcacgcgg cgcgatttcg
     1981 gccgggccgc gcgcatcgcg cacaacatca agggcagcgc gttctacatc cacagcgatc
     2041 cgatggccga cgacgccggc ggcctcgagc gcgcctgcga catcggcgac gacgccgccg
     2101 tcgaacgcca ctgggccgcg ctccaggaaa gcatcgacga ctggatgacc gcgcactgat
     2161 acgctag
//
LOCUS       DATCRO010000285         7124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_880685, whole genome shotgun sequence.
ACCESSION   DATCRO010000285 DATCRO010000000
VERSION     DATCRO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880685"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VIN75_07790"
     CDS             <1..569
                     /locus_tag="VIN75_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8974104.1"
                     /translation="FWADFYERLFNFREIRYFDIQGEYTGLTSKALTAPDGMIRIPLN
                     EESKKGSGQIEEFLMKFNGEGIQHIALLCDDLVPVIDKLQMAGVQLMTAPNDVYYEML
                     AERLPGHGQPVAELQSRGILLDGSTEGGKPRLLLQIFSQTHLGPVFFEFIQRKGDDGF
                     GEGNFKALFQSIERDQIRRGVLSAETAA"
     gene            830..1063
                     /locus_tag="VIN75_07795"
     CDS             830..1063
                     /locus_tag="VIN75_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974105.1"
                     /translation="MADALTSAQLEILRRIRDGAELTAPPFIPGMINELSFLRSFKLL
                     AFQRTFEAELTPLGRAYLAASERPGDVESAPGA"
     gene            complement(1088..1294)
                     /locus_tag="VIN75_07800"
     CDS             complement(1088..1294)
                     /locus_tag="VIN75_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974106.1"
                     /translation="MQADLFSARDAAALLAIRQLLDEGRLSRGALRAVFTAAGLMREM
                     DEAGPQPMQPLVGDRSGGHAKRAA"
     gene            complement(1372..1824)
                     /locus_tag="VIN75_07805"
     CDS             complement(1372..1824)
                     /locus_tag="VIN75_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974107.1"
                     /translation="MRHILRLAAFALAAALAAPAFASPTSDALSACLVDKSTGRDRKD
                     LARWIVVAVSSHPDMQDMFVLNQAMRETANKTAADIIMRLVTVECVDQARASAAVDGG
                     SSWGQAFGKLGEVAMKELTTNPAVATSFEDPVKYMDRAQLMKVFAPKP"
     gene            complement(1992..3737)
                     /locus_tag="VIN75_07810"
     CDS             complement(1992..3737)
                     /locus_tag="VIN75_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8974108.1"
                     /translation="MKLYAAEAEISTLETVLTQPWSEQVLSATVALAWYLRQRDSARA
                     LRLVDGVIPLLVPPPEGSRRPDAHRARAALAACEASALLCDFDVAEHWLSEARQHLDA
                     RVDALAEGDAWLVEAVVAKMRVQREREMAAHERAVAFFQGSGAPDRLAVAQVWLSFER
                     GYAQPDVDHSVGLQPPGPQWPEAAAAWQALWGASRGMALSFREPSESARMYRQAAASA
                     SAVGMVRLEIICMINAGAATQGLGDLDLAAECFEQAATRANATGWPTLIGTANTRVGE
                     LMGHLGAFEESRSILADAIVALSIGAPGLHLAIACASLAETLRAMGRAEESLEPMSRA
                     IALYREVGFTHTLALNLIFQARGLTAAGRPHEALAACDEARALIVANDLVSLHVGVTD
                     VLAELHHRFPLPAPAGMTLPSAALHYGEAALMEGLRVANWKAPGRLFTFLAERWAEAG
                     DHAKAYDYARRALEANEKESVQRMNYPLALVRLRKRAAQGETTGAGAASALHDAIGDR
                     PRRQASRTLTPKERSILLLLARDYSNKEIAKSIDVSDETVKWHLKNLFNKLEAGSRKH
                     AVTRARTLGFLSFTG"
     gene            complement(3902..4795)
                     /locus_tag="VIN75_07815"
     CDS             complement(3902..4795)
                     /locus_tag="VIN75_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8974109.1"
                     /translation="MKVSDTSSRQEPPFPGLVAFEATARLGSMSAAADELDLTQSAVS
                     QRVLKLEAHVGQRLFVRQGHGVRLTGAGELLMETAADTLQRLRAGFARIEPYRNKQSL
                     LLACPSDFAHGWLMPRLEALRAAHGGLEVWLMSEHDVMAIDRIDVDLVVSRRPLQGAD
                     IECVPLLQDRAVAVCAAALAARLQGVAFPKVLDKSPLLLLESAPEWGGVIPPASGRSA
                     RRRAATIQDERLLLEAAERGAGIAYVSQVLAADALASGRCVQLSQVPASARPPLWAMR
                     SRLTPRTPMADRVFDWLRAQA"
     gene            4873..5826
                     /gene="speB"
                     /locus_tag="VIN75_07820"
     CDS             4873..5826
                     /gene="speB"
                     /locus_tag="VIN75_07820"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8974110.1"
                     /translation="MNEAIRDTDHAIHRQSLLGYEMENTYSGVLSFMRRRYTRDLAGV
                     DVAVTGFPFDLATTFRTGARLGPAAIRAATVQLNEKIHPWGFGPCERLAIVDYGDCPL
                     DSHRPHTIVPSIVEHTRKIIASGATMLGLGGDHFITWPILQAMVEKHGAPLSLIHFDA
                     HCDTWQDDAPDSLNHGSMFYKAIKEGLVDPKRSVQVGIRTWNDDFMGVNVLGADWVHE
                     HGTKATVDRILQVVGANPSYLTFDIDCLDPAFAPGTGTPVAGGLSSAQAFAILRGLVP
                     VKIVGMDVVEVAPSYDHAEITAIAAAHVAAEMLCVLAAQRG"
     gene            6387..>7124
                     /gene="kdsA"
                     /locus_tag="VIN75_07825"
     CDS             6387..>7124
                     /gene="kdsA"
                     /locus_tag="VIN75_07825"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY8974111.1"
                     /translation="MSTVKITPTVECSNSAPFVLFGGINVLESKDLALRAAEEYVRVT
                     SKLGIPYVFKASYDKANRSSIHSYRGPGLEEGLRIFEAVKSAFGVPVITDVHEPHQAA
                     AVAQVVDVLQLPAFLARQTDLVVALAKTGRVINIKKPQFLSPSQMANICEKFREAGND
                     QLILCDRGTCFGYDNLVVDMLGFSVMKKTTGDAPVIFDVTHSLQQRESGAAASGGRRA
                     QVAELARAGMAVGLGGLFLEAHPDPDQA"
BASE COUNT         1086 a         2562 c         2466 g         1010 t
ORIGIN      
        1 cgttctgggc cgacttctac gagcgcctgt tcaacttccg cgagatccgc tacttcgaca
       61 tccagggcga gtacacgggc ctcacgtcca aggcgctgac ggcgccggac ggcatgatcc
      121 gcatcccgct gaacgaggaa tccaagaagg gctccggtca gatcgaggag ttcctgatga
      181 agttcaacgg cgagggcatc cagcacatcg cgctgctgtg cgacgacctc gtccccgtca
      241 tcgacaagct gcagatggcc ggcgtgcagc tgatgaccgc acccaacgac gtctactacg
      301 agatgctcgc cgagcgcctg cccggccacg gccagccagt cgcggaactg cagtcgcgcg
      361 gcatcctcct cgacggcagc accgagggcg gcaagccgcg cctgctgctg cagatcttct
      421 cgcagaccca cctgggcccg gtgttcttcg agttcatcca gcgcaagggc gacgacggct
      481 tcggcgaagg caacttcaag gcgctgttcc agtcgatcga acgcgaccag atccgccgcg
      541 gcgtgctcag cgccgagacg gctgcctgag cgtccgcacg cgcgacacgc ccgacccgcg
      601 gctgaagcag ggcctgacgt cgctggtcac gcacccgcac gactgcgtgc gcgaggtgcg
      661 cctgcctcgc gcgccggcgt tgagggtggc tggcacgtcg catcaaggat tgcctacaga
      721 cccgcccgcg gtccgctcgg aaaatcgcgc caacgactga cgaattgccc gcttcgggaa
      781 caacggcgcc gacggcgcac ggtcgcttcc accgaaggca acacgactca tggcagatgc
      841 actgaccagc gcgcagctgg agatcctgcg tcgcatccgc gacggcgccg aactcaccgc
      901 gccgcccttc attcccggca tgatcaacga gctgagcttc ctgcgctcgt tcaagctgct
      961 cgcgttccag cggacgttcg aagccgagct cacgccgctg gggcgcgcct acctggccgc
     1021 ctccgagcgg cccggcgacg tcgagtccgc gcccggggcg tgacggacgc gcaggccgtg
     1081 gccgcgttca ggccgcgcgc ttcgcgtgcc cgcccgagcg gtcgccgacg agcggctgca
     1141 tcggctgcgg acccgcctcg tccatctcgc gcatcaggcc cgcggcggtg aagaccgcgc
     1201 gcaacgcgcc gcgggacagg cgtccctcgt cgagcaactg ccggatggcg agcagcgcgg
     1261 cggcatcccg cgcggagaac aggtcggctt gcatgcctgt tgaatcggac gcgggcctgg
     1321 aggcctgaag gccgccggcg tggagaaggt cacgccgcgg cggcgtcttg ctcagggctt
     1381 gggcgcgaac accttcatca gctgggcgcg gtccatgtac ttgaccggat cctcgaacga
     1441 cgtcgccacg gccgggttgg tggtgagctc cttcatcgcc acttcgccca gcttgccgaa
     1501 ggcctgtccc caggacgagc cgccgtcgac ggcggccgag gcccgcgctt ggtcgacgca
     1561 ctcgaccgtg acgagccgca tgatgatgtc ggcggccgtc ttgttggcgg tctcgcgcat
     1621 cgcctggttg aggacgaaca tatcctgcat gtcggggtgc gaggacaccg cgacgacgat
     1681 ccagcgcgcg agatccttgc gatcgcggcc agtggacttg tcgaccaggc aggcgctgag
     1741 cgcgtccgag gtggggctgg cgaaggccgg ggcggcgagc gcggcggcca gcgcgaaggc
     1801 ggcgaggcgc aggatgtggc gcatggagat ctccctcttg gatgtcattg gatgtgcgga
     1861 ggaccgcggc gccattgtcg ccgcacgctg cttcaagggc ctggcgcggc ggcgccctcg
     1921 gcgagcagcc agctgcacaa tcgcggcacg ccctcgacca gtcgggccgt gtccttggcc
     1981 gcgaagcacg ctcagcccgt gaagctcagg aaccccagcg tccgcgcccg cgtgacggcg
     2041 tgcttgcgcg aacccgcctc gagcttgttg aacaggttct tcagatgcca cttcaccgtc
     2101 tcgtcgctga cgtcgatgga cttggcgatc tccttgttgg agtaatcgcg ggcgagcagc
     2161 agcaggatgc tgcgctcctt gggcgtcaag gtgcgcgagg cctggcgccg cgggcggtcg
     2221 ccgatggcgt cgtgcagggc cgatgcggcc ccggcgccgg tcgtctcgcc ctgcgcggcg
     2281 cgcttgcgca ggcgcaccag cgcgagcggg tagttcatcc gctgcaccga ctccttctcg
     2341 ttggcctcga gtgcccggcg tgcgtagtca taggccttgg catggtcgcc ggcctcggcc
     2401 cagcgctcgg ccaggaacgt gaacagccgg ccgggcgcct tccagttggc gacgcgcagg
     2461 ccttccatga gcgccgcctc gccgtagtgc agcgccgcgc tcggcagcgt catgccggcc
     2521 ggcgcgggca gagggaagcg atggtgcagt tccgcgagga catcggtcac gcccacgtgc
     2581 agcgagacga ggtcgttggc gacgatcagc gcgcgtgcct cgtcgcaggc ggccagcgct
     2641 tcatggggcc ggccggccgc ggtcaggccg cgggcctgga agatcaggtt cagcgcgagg
     2701 gtgtgcgtga agccgacctc gcggtacagc gcgatcgcgc ggctcatcgg ctcgagcgat
     2761 tcctcggcgc ggcccatcgc gcgcagcgtc tcggccagcg acgcgcaggc gatcgccagg
     2821 tgcaggcccg gcgcgccgat cgacagcgcc acgatcgcgt cggccaggat gctgcggctc
     2881 tcctcgaagg cgcccaggtg ccccatcagc tcgccgacgc gcgtgttcgc ggtgccgatc
     2941 agcgtcggcc agccggtggc gttggcgcgc gtggcggcct gctcgaagca ctcggcggcg
     3001 aggtcgaggt cgcccaggcc ctgcgtcgcg gcgccggcgt tgatcatgca gatgatctcc
     3061 agccgcacca tgcccaccgc ggaggcgctg gcggcggcct ggcggtacat gcgggccgac
     3121 tcggacggct cgcggaacga cagcgccatg ccgcgcgacg cgccccacag tgcctgccac
     3181 gcggcagcgg cctcgggcca ctgggggccg ggtggctgca gcccgacgct gtggtcgacg
     3241 tccggctgcg catagccgcg ctcgaagctg agccacacct gcgcgaccgc caggcgatcc
     3301 ggggcacccg agccctggaa gaacgcgacg gcgcgctcgt gcgcggccat ctcgcgctcg
     3361 cgctgcacgc gcatcttggc gacgaccgcc tccacgagcc aggcgtcgcc ttcggcgagc
     3421 gcgtcgacgc gcgcgtccag gtgctggcgc gcctccgaca gccagtgctc ggcgacatcg
     3481 aagtcgcaca gcagcgccga ggcctcgcac gccgcgagcg cggcgcgtgc gcggtgcgcg
     3541 tcggggcgcc ggctgccctc gggcggcggc accagcagcg gaatgacgcc gtcgaccagg
     3601 cgcagcgccc gcgcgctgtc acgctggcgc aggtaccagg ccagcgccac ggtggcagac
     3661 agcacctgct cggaccaggg ctgcgtcaac accgtttcga gcgtcgagat ctcggcttcg
     3721 gccgcgtaaa gcttcatttc ttgtattttc gcccgcggtc gagcagaaat ggccatgtcc
     3781 gacggggacc tcctgccaaa tccgccacgc gggagcgcgc gaaatgcggt ctgacggcgc
     3841 attgcatcgg cggagcggcg ggcgacgcct gcggtcaaac cgcccgacgg atcgaaggac
     3901 gtcaggcctg cgcacgcagc cagtcgaaca cgcggtccgc catcggcgtg cgcggcgtca
     3961 gccggctgcg catcgcccac agcggcggcc gcgcgctggc cggcacctgc gaaagctgca
     4021 cgcagcgccc gctggccagc gcgtcggcgg ccagcacctg cgagacatac gcgatgcccg
     4081 ccccgcgctc ggcggcctcg agcagcaggc gctcgtcctg gatcgtggcg gcccgccggc
     4141 gcgccgaccg gcccgatgcc ggcgggatca cgccacccca ctccggcgcg ctctccagga
     4201 gcagcagcgg cgacttgtcc agcaccttgg ggaaggccac gccctggagc ctggccgcca
     4261 gcgccgcggc gcacaccgcg acggcgcggt cctgcagcag cggcacgcat tcgatgtcgg
     4321 cgccctgcag cggccgacgc gagaccacca ggtcgacgtc gatgcgatcg atcgccatga
     4381 cgtcgtgctc ggacatcagc cacacctcca gcccgccatg cgcggcgcgc agcgcctcca
     4441 gccgaggcat caaccagccg tgcgcgaaat cggacgggca cgccagcagc agcgactgct
     4501 tgttgcgata gggctcgatg cgcgcgaagc ccgcgcgcag gcgctgcagc gtgtcggcgg
     4561 ccgtctccat cagcagctcg cccgcgcccg tcagtcgcac gccgtggccc tggcgcacga
     4621 acaggcgctg gccgacgtgg gcctcgagct tgagcacgcg ctggctgacg gccgactgcg
     4681 tcagatcgag ctcgtccgcc gccgccgaca tgctgcccag gcgcgcggtc gcctcgaacg
     4741 cgacgaggcc cgggaacggt ggttcctggc gggacgaggt atcagaaact ttcatatctc
     4801 ttccgaacga gctaatggcc cccgaggcgc cgtcctggga tcatccgggc ctggttccaa
     4861 cgagatttcc ccatgaacga agccatccgc gacaccgatc atgccatcca tcgccagtcc
     4921 ctcctgggct acgagatgga gaacacgtat tccggcgtct tgtccttcat gcgccgccgc
     4981 tacacccgcg acctggccgg cgtggacgtc gccgtcaccg gcttcccgtt cgacctggcc
     5041 accacgttcc gcaccggcgc gcgcctgggc ccggccgcga tccgtgcggc cacggtgcag
     5101 ctcaacgaga agatccaccc gtggggattc ggtccgtgcg agcgcctcgc catcgtcgac
     5161 tacggcgact gcccgctgga cagccaccgc ccgcacacga tcgtcccgtc catcgtggaa
     5221 cacacgcgca agatcatcgc gagcggcgcc acgatgctgg gcctgggcgg cgaccacttc
     5281 atcacctggc cgatcctcca ggcgatggtc gagaagcacg gcgcgccgct gtcgctgatc
     5341 cacttcgacg cgcactgcga cacctggcag gacgacgcgc ccgattcgct gaaccacggc
     5401 tcgatgttct acaaggccat caaggaaggc ctggtcgacc cgaagcgctc ggtgcaggtg
     5461 ggcatacgca cgtggaacga cgacttcatg ggcgtgaacg tgctgggcgc cgactgggtg
     5521 cacgagcacg gcacgaaggc gacggtcgac cgcatcctgc aggtcgtcgg cgcgaacccg
     5581 agctacctga ccttcgacat cgactgcctg gaccccgcgt tcgcgcccgg caccggcacg
     5641 ccggtggccg gcggcctgag cagcgcgcag gcgttcgcga tcctgcgcgg cctcgtgccg
     5701 gtgaagatcg tcggcatgga cgtcgtcgag gtcgcgccgt cgtacgacca cgccgagatc
     5761 accgccatcg ccgccgccca cgtggccgcg gagatgcttt gcgtgctggc cgcgcagcgc
     5821 ggctgagcgc gtgcgcccgc gcgtgacgcg cgagctcggc ggccgctgaa gggcagcacg
     5881 aggtacagct tgccgagtcg gccggtcagc tggccggcaa ggccgtcgcc ggaccagtgg
     5941 aaggcgaggc aatcgacccg cgggctgcgg ccgccgtgcg cagccgatcc atggcctgtc
     6001 tccgggtgct ttcgttcgaa agaggccggg gatcgctccc cggccgaaga cgtcccaacg
     6061 acgccctaag ttgcctgtca agcggatcgc aagtcccatg cccgcggcgc ccggtcgtat
     6121 ccaaatgaaa ggccgggggc ccgcttcgtc tggaaccggt tccggaacag ttgtccagct
     6181 ccacggcgcg ggctgacggc ggaccagatt tgtcacaccc gcgggtgacc aatcgggtca
     6241 cttcgggcgc gcggctgacg tggctggtcg ccccggcggc ggcggccgta tccattcgtg
     6301 acggcgccag cggccgagga atgccggccc ggcgggccgc cgcggcgcgc cgttgcaaaa
     6361 tgccggttgc gaaccaagga gacgccatgt ccaccgtcaa gatcaccccc accgtcgagt
     6421 gcagcaacag cgcgccgttc gtgctgttcg gcggcatcaa cgtgctggag tcgaaggacc
     6481 tcgccctgcg cgcggccgag gaatacgtgc gcgtcacgag caagctgggc atcccctacg
     6541 tgttcaaggc cagctacgac aaggccaacc gctcgtcgat ccactcgtac cgcggccccg
     6601 gcctggagga aggcctgcgc atcttcgagg cggtcaagtc ggccttcggc gtgcccgtca
     6661 tcaccgacgt gcacgagccg caccaggccg ccgccgtcgc gcaggtggtc gacgtgctgc
     6721 agctgcccgc cttcctcgcg cgccagaccg atctggtcgt ggcgctggcg aagaccggcc
     6781 gcgtcatcaa catcaagaag ccgcagttcc tgagcccgtc gcagatggcc aacatctgcg
     6841 agaagttccg cgaggccggc aacgaccagc tgatcctgtg cgaccgcggc acctgcttcg
     6901 gctacgacaa cctggtcgtc gacatgctcg gcttctccgt gatgaagaag acgaccggcg
     6961 atgcgccggt gatcttcgac gtcacgcact cgctgcagca gcgcgagtcc ggcgcggcgg
     7021 cttccggcgg gcggcgcgca caggtcgcgg agctcgcgcg cgcgggcatg gcggtcggcc
     7081 tgggcgggct gttcctggag gcccatcccg atcccgacca ggcc
//
LOCUS       DATCRO010000286         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_81871, whole genome shotgun sequence.
ACCESSION   DATCRO010000286 DATCRO010000000
VERSION     DATCRO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81871"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VIN75_07830"
     CDS             <1..690
                     /locus_tag="VIN75_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEY8974112.1"
                     /translation="LPADASADANPEDVPNALSQLAREGLVEPCDPSDGDPEPPDITR
                     DPPAYPLPRTGWLQMLARADEGWVLGMGYSTQRGYAHTHPFAGDLRSGKVAVELVPDE
                     LDFAIEIGEVELTECQMVNQFIGSAEVAPVFTRGQGLVFGRGERKAIAMSLVDRALRA
                     AEFGEDATSPAQQQEFALPQGDSVEASGFVQHLKLPHYVTFESELQLIRKLREDVHAR
                     AAGAARQGEPE"
     gene            797..1639
                     /locus_tag="VIN75_07835"
     CDS             797..1639
                     /locus_tag="VIN75_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEY8974113.1"
                     /translation="MMQGYNFAYLDERTKKMIRRALLKAVAIPGHQVPFGSREMPLAY
                     GWGTGGIQVTAAVIGQDDVLKVIDQGSDDTTNAVNIRQFFARTTGVRTTTRTRDATLI
                     QTRHRIPETPLVDGQVLVYQVPQPEPLYRLEARRAETTKLHAAEEYGLMNVKLYEDLA
                     QWGRVETSYDYPVKVDGRYVMSPSPIPAFDNPKMHMSPALQLFGAGREKRLYAIPPYT
                     EVRSLDFEDHPFRIEPHGQACARCGDTTSFLDEVADAEGGVRWTCSDTDWCADRVDQG
                     HGHE"
     gene            1632..2417
                     /gene="phnK"
                     /locus_tag="VIN75_07840"
     CDS             1632..2417
                     /gene="phnK"
                     /locus_tag="VIN75_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEY8974114.1"
                     /translation="MSEPLLRVRGLSHRFAAARDERFAVRDAELDLFPGEVVAIVGES
                     GSGKSTLLNCIAGRIAPTHGQVHYRDADGRWLDVHALTERQQRVLARTEWGFVEQNAS
                     DGLRLDVSAGANIGERLMGQGDRHYGRLRATARRWMERVELDTARIDDSPRAFSGGMR
                     QRLQIARNLVTRPRLVLLDEPTSGLDVSVQARLLDLIRMLTTRMHLCALIVTHDLGVA
                     RLLAQRTLVMRHGQVVEQGLTDRVLDDPRHPYTQLLVSSVVMP"
     gene            2429..>2855
                     /locus_tag="VIN75_07845"
     CDS             2429..>2855
                     /locus_tag="VIN75_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974115.1"
                     /translation="MPRLSVRGLAKHFTLHLRGGARLQVLEDTALDVRAGECVALVGA
                     SGSGKSTLMKCLFGSYGSDAGTLEFDSSDGRVDIAAATPQRMLALRRRDIAYVSQFLR
                     AVPRVPALDVVAQRLVDADADAAPASDDLDDDAHARQMAA"
BASE COUNT          442 a         1024 c         1009 g          380 t
ORIGIN      
        1 ctgccggccg acgcatccgc cgacgcgaac cccgaggacg tccccaacgc gttgtcgcaa
       61 ctggcccgcg aaggcctggt cgagccgtgc gacccgtccg acggcgaccc cgagccgccg
      121 gacatcacgc gcgatccgcc cgcctatccg ctgccgcgga ccggctggct gcagatgctg
      181 gcgcgcgccg acgagggctg ggtgctgggc atgggctact cgacgcagcg cggctacgcg
      241 cacacgcacc cgttcgcggg cgacctgcgc tcgggcaagg tggcggtgga gctggtgccc
      301 gacgagctcg acttcgcgat cgagatcggc gaggtcgagc tcaccgaatg ccagatggtc
      361 aaccagttca tcggcagcgc cgaggtcgcg cccgtgttca cgcgtggcca gggcctcgtc
      421 ttcgggcgcg gcgagcgcaa ggcgatcgcg atgtcgctgg tcgaccgcgc gctgcgcgcc
      481 gcggagttcg gcgaggacgc gaccagcccc gcgcagcagc aggaattcgc gctgccacaa
      541 ggcgacagcg tcgaggcctc gggcttcgtg cagcacctga agctgccgca ctacgtgacc
      601 ttcgagtcgg agctgcagct catccgcaag ctgcgcgagg acgtgcatgc gcgggcggcc
      661 ggcgccgctc gccagggaga gccggaatga cgaacgaaag gcgaaagcac gcagccgccg
      721 cgcccggccg ctccggaaca ggggcgcgcc cgtccttgga gggctggcag cggtactccg
      781 ctgacggggg gctcacatga tgcaagggta caacttcgcc tatctcgacg aacgcacgaa
      841 gaagatgatc cgccgcgccc tgctcaaggc cgtcgcgatc cctggccacc aggtgccgtt
      901 cggctcgcgc gagatgccgc tggcctacgg ctggggaacg ggcggcatcc aggtgacggc
      961 ggcggtgatc ggccaggacg acgtgctcaa ggtgatcgac cagggcagcg acgacaccac
     1021 gaacgccgtg aacatccgcc agttcttcgc gcgcacgacg ggcgtgcgca cgaccacgcg
     1081 cacgcgcgac gccacgctga tccagacgcg ccatcgcatc cccgagacgc cgctcgtcga
     1141 cggccaggtg ctcgtgtacc aggtgccgca gcccgagccg ctgtaccgcc tggaagcgcg
     1201 ccgcgccgag acgacgaagc tgcacgcggc cgaggaatac gggttgatga acgtcaagct
     1261 gtacgaggat ctggcgcagt ggggccgcgt cgagacgagc tacgactacc cggtgaaggt
     1321 ggatggccgc tacgtgatgt cgccctcgcc gatccccgcg ttcgacaacc cgaagatgca
     1381 catgagcccc gcgctgcagc tgttcggcgc gggccgcgag aagcgcctct acgcgatccc
     1441 gccgtacacc gaggtgcgca gcctcgactt cgaggatcac ccgttccgca tcgagccgca
     1501 cggccaggcc tgcgcgcgct gcggcgacac caccagcttc ctcgacgagg tggccgacgc
     1561 cgagggcggc gtgcgctgga cctgctctga caccgactgg tgcgccgatc gcgtcgacca
     1621 aggacatggc catgagtgaa cccctgctgc gcgtgcgcgg cctgtcgcac cgtttcgccg
     1681 ctgcgcgcga cgagcgcttc gccgtgcgcg atgccgaact cgacctgttc ccgggcgagg
     1741 tggtcgccat cgtcggcgaa tcggggtcgg gcaagtcgac cctgctgaac tgcatcgccg
     1801 gccgcatcgc gcccacccac ggccaggtgc actaccgcga cgcggacggt cgctggctgg
     1861 acgtgcacgc cctgacggag cgccagcagc gcgtgctcgc gcgcaccgaa tggggcttcg
     1921 tcgagcagaa cgcgagcgac gggctgcggc tggacgtgag cgcgggcgcc aacatcggcg
     1981 aacggctgat ggggcagggc gaccgccact atggccggct gcgcgcgacg gcgcgacgct
     2041 ggatggagcg cgtggagctc gataccgctc gcatcgacga ctcgccacgc gccttctccg
     2101 gcggcatgcg ccagcgcctg cagatcgcgc gcaacctggt cacgcggccg cggctcgtgc
     2161 tgctggacga accgacctcg ggcctggacg tgtccgtgca ggcgcgcctg ctcgacctca
     2221 tccgcatgct gaccacgcgc atgcacctgt gcgcgctgat cgtcacgcac gacctcggcg
     2281 tggcgcgcct gctggcgcag cgcacgctgg tgatgcgcca cgggcaggtc gtcgagcagg
     2341 ggctcaccga ccgcgtgctc gacgaccccc ggcacccgta cacgcaactg ctggtctcct
     2401 cggtggtgat gccatgaacg aagattccat gccgcgcttg tccgtgcgcg gcctcgccaa
     2461 gcacttcacg ctgcacctgc gcggcggggc acgtcttcag gtgctcgagg acaccgcgct
     2521 cgacgtgcgc gccggcgagt gcgtcgcact ggtgggcgcg tcgggcagcg gcaagtcgac
     2581 gctgatgaag tgcctgttcg gcagctacgg cagcgacgcc ggcacgctgg aattcgactc
     2641 gtcggacggc cgggtcgaca tcgccgccgc cacgccgcag cgcatgctgg ccctgcgccg
     2701 ccgcgacatc gcctacgtca gccagttcct gcgcgcggtg ccgcgcgtgc cggcgctgga
     2761 cgtcgtcgcg cagcggctgg tcgacgccga cgccgacgcc gcgcctgcgt cggacgacct
     2821 cgacgacgac gcgcatgcgc ggcagatggc agcgg
//
LOCUS       DATCRO010000287         6379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1060673, whole genome shotgun sequence.
ACCESSION   DATCRO010000287 DATCRO010000000
VERSION     DATCRO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060673"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VIN75_07850"
     CDS             complement(<1..1138)
                     /locus_tag="VIN75_07850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8974116.1"
                     /translation="MRWNLFRRADSAGASDSNFETTVVDDAAAARLAQGNTAETEGEI
                     TVAPPPPVLAGIGPGTEGESIGHVGRYALKSVLGKGGLGTVYNAWDPLLSRTVALKTL
                     HIDSDPASRESLDAMFLNEARAAAGLSHPHIVTVFDAGPSDSGVYIAMERLQGQDLRH
                     LLLQGVRPGPIEAAQVVRRVADALAYAHRKGVVHCDVKPANIFMTGRMHPKVLDFGIA
                     RVAHRDVPALDGVVAGSPYYLAPEQVDGRSVDRRSDVYSLGVVLYELLVGKKPFEGKS
                     LEEITAAVKAGDAVPAHRVSPKIPLGLSVIASRAMAADPDQRYPSARHLSMELRHWVD
                     SAEARALKDEARGVSAPAPTKNPWLRIGLPLVGFVAVIGVLGWLK"
     gene            complement(1272..2456)
                     /locus_tag="VIN75_07855"
     CDS             complement(1272..2456)
                     /locus_tag="VIN75_07855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8974117.1"
                     /translation="MKLAGRLAHIDPFYVMECAKVAQEIARSPACDPAQGGEPMIYLN
                     IGEPDFTAPAPVIAAAEAALHAGRTQYTNALGLPALRECISQWYASRWGLDVPARRIV
                     VTAGASAGLQLVCQALFEAGDEVLMPDPSYPCNRHFVAATGAAARLVPTTPAMRFQLD
                     AATVSAHWQPQTRGVLLATPSNPTGTSIAPDELARIVDVVRGHDGIAIVDEIYQGLSY
                     DPAFERCALALGDDIVSVNSFSKYFSMTGWRLGWLVLPEALIEPVERLAQNLYICASA
                     VAQHAAIAAFDAESLAECEIRRDAFRQRRDFIVPALQKLGLDVPVVPDGAFYVWADCR
                     RFAADSWDFAMQLMRSAHVALTPGKDFGPAHASTYFRLSFATALPQLRETVRRLEREL
                     VR"
     gene            complement(2461..2931)
                     /gene="nusB"
                     /locus_tag="VIN75_07860"
     CDS             complement(2461..2931)
                     /gene="nusB"
                     /locus_tag="VIN75_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8974118.1"
                     /translation="MNSPEKAEKPAKPKSARRRSREVALQGLYEWLVSGTDAGVIDAH
                     MREQEGFDKADRAHFDALLHGCIAEAADLDGVLARFVDRKTTELSPVEHGVLMIGAYE
                     LKHCIDIPYKVAINEAVELAKSFGGTDGHKYVNGVLDKAAAVLRATEVQASHKR"
     gene            complement(3038..3502)
                     /gene="ribH"
                     /locus_tag="VIN75_07865"
     CDS             complement(3038..3502)
                     /gene="ribH"
                     /locus_tag="VIN75_07865"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8974119.1"
                     /translation="MQAADQGTATDLDGRDLVIGIVRARFNDAITSRMADICHAELLA
                     LGVSAKHVTTITVPGALEVPIALKAMADQDDFDALVAIGCVIRGETYHFELVANESGA
                     GVTRVSLDHQVPIANAILTVENEAQALARVEDKARDAARVAVEMANLMDDLL"
     gene            complement(3661..4767)
                     /gene="ribBA"
                     /locus_tag="VIN75_07870"
     CDS             complement(3661..4767)
                     /gene="ribBA"
                     /locus_tag="VIN75_07870"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY8974120.1"
                     /translation="MPISPVPALVNELAAGRMIILVDEEDRENEGDLVLAAEFVTPEA
                     INFMARHARGLICLTMTREMCERLHLPPMATRNGSPHGTAFTVSIEAAEGVTTGISAA
                     DRARTVQAAVARGAKPSDLVQPGHIFPLQAQEGGVLMRAGHTEAGCDMAAMAGLVPSA
                     VICEVMNEDGTMARLPDLEIFAKEHGLLIGTIADLIHHRSRNESLISRIGQRELATPY
                     GRFDCHLFRDKSGGAHMALVKGTWKAGEEVPVRVHEPFSAIDLLDSSCGKHSWPLPQA
                     LAAIEKRGQGVAVLLNCGEPADHLVAQLMPESDPCEPVAKRPMDLRTYGVGAQILREL
                     GVTRMQLLGQPRRMPSMTGYGLEVSSFITAADAA"
     gene            complement(4814..5920)
                     /gene="ribD"
                     /locus_tag="VIN75_07875"
     CDS             complement(4814..5920)
                     /gene="ribD"
                     /locus_tag="VIN75_07875"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY8974121.1"
                     /translation="MKPWLLTLALAEQSVGLTDPNPRVGCVIVSADGSAVLGQGHTQA
                     AGGPHAEVMALRDAQARGHDVRGATVYVTLEPCAHHGRTPPCCDALVAAGVGRVEIAT
                     GDPNPLVNGQGTARIAAAGIAIGHAPEDVARRARELNIGFFSRMQRGRPWVRMKIAAS
                     LDGRTALPNGASQWITGADARADGHAWRRRAGAVLTGIGTVLADDPSLDVRLVETARQ
                     PMRCVVDARLDTPPDARLFETASPVILYTASTDTARRAPLEERGAEIVDVPSNHGRLD
                     LEAVLANLAARHVNELHVEAGARLNGALLRTGQVDELLLYLAPTLLGAGRDMAALGPF
                     EALTQGLAFEFHDAARVGADLRILARNRSVTGTP"
     gene            6050..>6379
                     /locus_tag="VIN75_07880"
     CDS             6050..>6379
                     /locus_tag="VIN75_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY8974122.1"
                     /translation="MPVLPVLPVLPLLARRRPRPGSPPAAVRGFTLVELAVVLAIVAV
                     LLRVATPGLSRGAAARALAAQASEFMAALRFARAQALARGAAVTICASAPGADGPACQ
                     RARSADWR"
BASE COUNT          869 a         2196 c         2359 g          955 t
ORIGIN      
        1 acttgagcca gccgagcacg ccgatgaccg cgacgaagcc gaccagcggc aggccgatgc
       61 gcagccacgg gttcttcgtc ggcgccgggg cgctgacgcc gcgggcctcg tccttgagcg
      121 cgcgtgcctc ggccgagtcg acccagtggc gcagttccat cgacaggtgg cgcgccgacg
      181 ggtagcgctg gtcggggtcg gcggccatcg cgcggctggc gatcacggac agccccagcg
      241 ggatcttggg cgagacgcgg tgcgcgggga cggcgtcgcc ggccttgacg gcggcggtga
      301 tctcctcgag cgacttgccc tcgaacggct tcttgcccac cagcagctcg tacagcacga
      361 cgcccagcga atagacgtcg ctgcggcgat ccaccgagcg gccgtcgacc tgctcgggcg
      421 cgaggtagta cggcgagccg gcgaccacgc cgtcgagcgc gggcacgtcg cggtgcgcga
      481 cgcgcgcgat gccgaagtcc agcaccttgg ggtgcatgcg gccggtcatg aagatgttgg
      541 ccggcttgac gtcgcagtgg acgacgccct tgcggtgcgc gtaggccagg gcgtcggcca
      601 cgcggcgcac tacctgggcc gcctcgatcg ggcccgggcg cacgccctgc agcagcaggt
      661 ggcgcaggtc ctggccctgc agccgctcca tggcgatgta gacgccgctg tcggacggac
      721 cggcgtcgaa cacggtgacg atgtgcgggt gcgacaggcc ggccgcggcg cgcgcctcgt
      781 tcaggaacat cgcgtccagc gactcgcgcg aggccgggtc ggagtcgatg tgcagcgtct
      841 tgagcgcgac cgtgcgcgac agcagcggat cccacgcgtt gtagacggtg cccagcccgc
      901 ccttgcccag gacggacttc agcgcgtagc ggccgacgtg gccgatgctc tcgccttccg
      961 tgccggggcc gatgccggcc agcaccggcg gcggcggcgc gaccgtgatc tcgccttcgg
     1021 tctcggccgt gttgccctgc gcgaggcgag cggcggccgc gtcatcgacg acggtcgtct
     1081 cgaaattgga gtcggaggcg cccgccgaat cggcgcgcct gaaaagattc catcgcatgg
     1141 gcgaaagtta accgatgggc gccccgtcga agcgcggaat aaccggaaga tacccccgac
     1201 gatgacacaa atatcaccgg tctggcgaat gcgtcacgct tgccgggagg gttgaaagag
     1261 gcttgaaagg gtcaacggac gagctcgcgt tccagccggc gcaccgtttc ccgcagctgc
     1321 ggcaacgccg tcgcgaacga caggcggaag tatgtgctcg catgcgcggg gccgaagtcc
     1381 ttgcccgggg tcagggccac gtgcgcgctg cgcatcagct gcatcgcgaa atcccagctg
     1441 tcggccgcga agcggcggca atcggcccac acgtaaaagg ccccgtcggg aaccaccgga
     1501 acgtccagcc ctagtttctg cagcgcgggg acgatgaaat cgcggcgctg gcggaaggca
     1561 tcccggcgaa tctcgcattc ggccaggctt tcggcgtcga aggcggcgat cgcggcatgc
     1621 tgggcgaccg cgctcgcgca gatgtagagg ttctgcgcca gccgttcgac gggctcgatc
     1681 agcgcctcgg gcagcaccag ccagcccagg cgccagccgg tcatgctgaa gtacttcgag
     1741 aagctgttga cggagacgat gtcgtcgccc agcgcgagcg cgcagcgctc gaaggccggg
     1801 tcgtagctca ggccctggta gatctcgtcg acgatggcga tgccgtcgtg cccgcggacg
     1861 acgtcgacga tgcgcgccag ttcgtccggc gcgatcgacg tgccggtcgg attcgacggc
     1921 gtggccagca ggacgccgcg cgtctgcggc tgccagtggg cgctcaccgt cgcggcgtcg
     1981 agctggaagc gcatggctgg cgtcgtgggc accagccgcg ccgctgcgcc cgtggcggcc
     2041 acgaagtgcc ggttgcacgg gtagctcgga tcgggcatca gcacctcgtc gccggcctcg
     2101 aacagcgcct ggcagaccag ctgcagtcct gccgaagcgc ccgccgtgac gacgatgcgc
     2161 cgcgccggca cgtccagccc ccagcggctc gcgtaccact ggctgatgca ttcgcgcagc
     2221 gcgggcaggc ccagggcgtt cgtgtactgc gtgcggccgg cgtgcagcgc ggcctcggcg
     2281 gcggcgatga ccggcgccgg cgcggtgaag tccggttcgc cgatgttcag gtagatcatc
     2341 ggctcgccgc cctgcgcggg atcgcaggcg ggtgaacgcg cgatctcctg cgccaccttg
     2401 gcgcactcca tcacgtagaa cggatcgatg tgggcgaggc ggccggcgag cttcatgccg
     2461 tcaacgcttg tgcgaggcct gcacctcggt cgcccgcagc acggcagcgg ccttgtccag
     2521 cacgccgttg acgtacttgt ggccgtcggt gccgccgaag ctcttggcca gttcgacggc
     2581 ctcgttgatc gcgaccttgt acgggatgtc gatgcagtgc ttgagctcgt aggcgccgat
     2641 catgagcacg ccgtgttcga ccggcgacag ctcggtcgtc ttgcggtcga cgaaccgggc
     2701 cagcacgccg tccaggtcgg cggcctcggc gatgcagccg tgcagcagcg cgtcgaagtg
     2761 cgcgcggtcg gccttgtcga agccttcctg ctcgcgcatg tgggcgtcga tcacgccggc
     2821 gtcggtgccg gagaccagcc actcgtacag gccctgcagc gcgacctcgc gcgaacggcg
     2881 gcgcgccgac ttgggcttgg ccggcttctc ggccttctcg ggggaattca ttggagcccc
     2941 tcccgcgccg agtacgtcgc tccgtccccc gaggggactg cgggcctcct tggggcggcc
     3001 cggcgcacgg cccgcaaaca ggttggcacg gaattcttca tagcaggtcg tccatcaggt
     3061 tggccatctc cacggccacg cgcgcggcgt cgcgcgcctt gtcctcgacg cgggccagcg
     3121 cctgggcctc gttctcgacg gtcaggatcg cgttggcgat cggcacctgg tggtcgagcg
     3181 acacgcgcgt gacgcccgcg ccgctctcgt tggccaccag ttcgaagtgg taggtctcgc
     3241 cgcggatcac gcagccgatg gccacgagcg cgtcgaagtc gtcctggtcc gccatcgcct
     3301 tcagcgcgat cggcacttcg agcgcgccgg gcacggtgat cgtcgtgacg tgcttggccg
     3361 acacgcccag cgcgagcagc tcggcgtggc agatgtcggc catgcgcgag gtgatcgcgt
     3421 cgttgaagcg ggcgcgcacg atcccgatga cgaggtcgcg gccgtccaga tcggtggcgg
     3481 tgccttggtc tgctgcttgc atcaggaaac gctccaaatg aaaagaacaa tgcgccgggt
     3541 ggcgcgtgat ggttttgctt ttccccaagc tctcgggagg cgaggatcgc cacccgagcc
     3601 tgaccgagca ctccctcggg gggcagcgac cggagggagc gtgggggcaa acatctcttg
     3661 tcaggcggcg tcggccgccg tgatgaaact ggacacttcc agaccgtagc ccgtcatgct
     3721 gggcatgcgg cgtggctgcc cgagcagctg catgcgcgtg acgcccagct cgcgcaggat
     3781 ctgggcgccg acgccatagg tgcgcaggtc catcggccgc ttggccacgg gctcgcacgg
     3841 gtcggactcg ggcatcagct gggcgaccag gtggtcggcc ggctcgccgc aattgagcag
     3901 caccgccacg ccctggccgc gcttctcgat ggcggccagc gcctgcggca gcggccacga
     3961 gtgcttgccg cacgacgagt cgagcaggtc gatcgccgag aacggctcgt gcacgcgcac
     4021 cggcacttcc tcgccggcct tccaggtgcc cttgacgagc gccatgtgcg cgccgccgga
     4081 cttgtcgcgg aacaggtggc agtcgaagcg gccgtacggc gtggccagct cgcgctggcc
     4141 gatgcgcgag atcagcgact cgttgcggct gcggtggtgg atcaggtcgg cgatggtgcc
     4201 gatcagcagg ccatgctcct tggcgaagat ctcgaggtcg ggcaggcgcg ccatcgtgcc
     4261 gtcctcgttc atcacctcgc agatcaccgc ggacggcacg aggccggcca tcgccgccat
     4321 gtcgcagccg gcctcggtat ggccggcgcg catcagcacg ccgccttcct gggcctgcag
     4381 cgggaagatg tggcccggct gcacgaggtc ggacggcttg gcgccgcgcg cgacggcggc
     4441 ctgcacggtg cgggcgcggt cggcggcgga gatgccggtg gtcacgccct cggcggcctc
     4501 gatggacacg gtgaacgcgg tgccgtgcgg cgagccgttg cgcgtggcca tcggcggcag
     4561 gtgcaggcgc tcgcacatct cgcgcgtcat cgtcaggcag atcaggccgc gcgcgtggcg
     4621 ggccatgaag ttgatcgcct cgggcgtcac gaattcggcc gcgaggacca ggtcgccttc
     4681 gttttcgcgg tcttcctcgt cgacgaggat gatcatgcgg cccgcggcca attcgttgac
     4741 cagggcggga acaggagaga tgggcatccc gcgattgtag gcggcggctc cgggtttccc
     4801 aggagcaagg gctctacggc gtgccggtta cagaccggtt ccgtgcaagg atgcgcaggt
     4861 ccgctcccac tctggccgcg tcgtggaact cgaacgccag gccctgcgtg agcgcctcga
     4921 acgggccgag cgccgccatg tcgcggccgg cgcccagcag cgtgggcgcc aggtacagca
     4981 gcagctcgtc gacctggccg gtgcgcagca gcgccccgtt gaggcgagcg cccgcttcga
     5041 cgtgcagttc gttgacgtgg cgcgcggcga ggttggccag cacggcctcg aggtccaggc
     5101 ggccgtggtt gctcgggacg tcgacgatct cggccccgcg ttcctccagc ggggcgcggc
     5161 gcgcggtgtc ggtggacgcg gtgtagagga tcacgggcga ggccgtctcg aacaggcggg
     5221 cgtcgggcgg ggtgtccagt cgcgcgtcga ccacgcagcg catcggctgc ctggcggttt
     5281 cgacaagacg gacgtccagc gacggatcgt cggccagcac ggtgccgatg ccggtgagca
     5341 cggcgccggc ccgcctgcgc caggcatggc cgtcggcgcg cgcgtcggcg ccggtgatcc
     5401 attggctggc gccgttgggc agggcggtgc ggccgtccag cgacgccgcg atcttcatgc
     5461 gcacccaggg gcggccgcgc tgcatgcgcg agaagaagcc gatgttgagc tcgcgggcgc
     5521 gccgcgccac gtcctcgggc gcatggccga tcgcgatgcc ggccgcggcg atgcgcgccg
     5581 tgccctggcc gttgaccagc gggttggggt cgccggtggc gatctcgacg cggccgacgc
     5641 ccgcggccac gagggcgtcg cagcagggcg gcgtgcggcc gtggtgtgcg caaggctcca
     5701 gcgtgacgta gacggtggcg ccgcgcacgt cgtggccgcg cgcctgcgcg tcgcgcaggg
     5761 ccatcacctc ggcgtgcggc ccgccggcgg cctgcgtgtg gccctgcccc agcacggccg
     5821 aaccgtccgc cgacacgatc acgcagccca cgcgcgggtt cgggtcggtc aggccgaccg
     5881 actgctcggc gagcgccagc gtcaaaagcc agggtttcat cgtgatcgtc cggttggccg
     5941 cgcgcccagg cgccggtttt cgagcgggat ggcgaaggcg gccgtcccgg ggggtgattt
     6001 tccacgacgg cgcctgaaca atcggcggcc cgttcgtcga gtcagaccca tgcctgtcct
     6061 gcctgtcctg cctgtcctgc ccttgctcgc ccggcgccgt ccgcggcccg gttccccgcc
     6121 cgccgccgtc cgcggcttca cgctggtcga actggccgtc gtgctggcga tcgtcgccgt
     6181 cctgctgcgc gtggccacgc cgggcctgtc gcgcggcgcg gcagcgcgcg cgctcgcggc
     6241 gcaggcctcc gaattcatgg cggcgctgcg cttcgcccgc gcccaggcgc tggcgcgcgg
     6301 cgccgcggtc acgatctgcg ccagcgcgcc gggcgcggac gggccggcct gccagcgtgc
     6361 gcgctcggcc gattggcgg
//
LOCUS       DATCRO010000288         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_610798, whole genome shotgun sequence.
ACCESSION   DATCRO010000288 DATCRO010000000
VERSION     DATCRO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610798"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /gene="rodA"
                     /locus_tag="VIN75_07885"
     CDS             <1..1044
                     /gene="rodA"
                     /locus_tag="VIN75_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8974123.1"
                     /translation="TTMYSAGYDSGTRFTDHGRNMLLALGVLFVAAQISPQNLMRVAV
                     PLYSVGLALLLATAVMGITKKGATRWLNVGVVIQPSEIMKLAMPLMLAWWFQKREGQL
                     RALDFAIAGLILLLPVGLVVKQPDLGTAILILSAGLYVIFFAGLSWKLILPVLLLAAV
                     GIVAVVWSEDRICQDSVVWPLLRDYQKHRICTLLDPTTDPLGKGFHIIQGMIAIGSGG
                     VTGKGFMSGTQTHLEFIPERTTDFIFAAYSEEFGMLGALLLLFGFTLLIFRGLAIAAD
                     APSVFTRLLAGSITLSFFTYAFVNMGMVSGILPVVGVPLPFVSYGGTAMVTLGLGLGF
                     LTSIARNRRSVQT"
     gene            complement(1119..2816)
                     /locus_tag="VIN75_07890"
     CDS             complement(1119..2816)
                     /locus_tag="VIN75_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8974124.1"
                     /translation="MRPRSWFAEDGAPVQEASGPRTGPGRRPGLGFVLEAAWIALLVA
                     PAASRLPDDLAWQLDDVARIVGPLWLVALLRLLLPRRAFFPATWPIAAAGVLVVGASL
                     LRAVDALDLLLQLRTYSSAELEEALSPYAGMALGSLVLVSALAWGAWRHAASRSPTRR
                     LRVAAVVATLAAALVLPATTLVKLWPLEPALATASVATNAHWLANRLYPEASTFDPRD
                     PAASWHATRAPGASARETVVMVVGESVRDDYLKECDGPALVRPVDADALVACDVVAGA
                     DQTSISVPLLISRELPGHPVRVSSDGSFLRALDETGFATHWIALNRQRRDVAWPDAAR
                     VDFTTSAHDTDAWLLPALDASLREPASLKGIVLHGYNAHDPYCERFDAATAPYPVDCS
                     ARGQHWRKETVATRRQAYADAVDASIGFVNQVIARLRQQREPVFLVYSSDHGDALMDD
                     GRQIVGHARRYPTRWDSHVPAVFWANDAWRRLHAAQWARLAAQKGRPLMHMDLVPTLL
                     DAAGVRYDEPRTQAVDLLAQPVPERQRVVQVARGRAVAWQALVDEARAAGPAAPGGR"
     gene            complement(2944..>3502)
                     /locus_tag="VIN75_07895"
     CDS             complement(2944..>3502)
                     /locus_tag="VIN75_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8974125.1"
                     /translation="FDPHTAPYAVDCLLPAEDLDPISLRRLRLSYANAVDATVGFLDE
                     VIAQLRQRPEPVFFVYTSDHGDALLDDSRQIWSHGLRHPTRWDVQVPAVFWANDAWRA
                     THAAQWANLQAQVGAPLMHADVVPTLLAAADVRYDEPRRVPVDLLARAVPPRRRDVQV
                     RLGETISWQTLVDEAREAGPYVPHY"
BASE COUNT          474 a         1263 c         1286 g          479 t
ORIGIN      
        1 acgacgatgt attcggccgg ctacgactcc ggcacgcgct tcaccgacca cgggcgcaac
       61 atgctgctcg cgctcggcgt gctgttcgtc gcggcgcaga tctcgccgca gaacctgatg
      121 cgcgtcgcgg tgccgctgta ctcggtcggg ctggcgctgc tgctggccac cgcggtgatg
      181 ggcatcacga agaagggcgc cacgcgctgg ctcaacgtag gcgtcgtcat ccagcccagc
      241 gagatcatga agctcgcgat gccgctgatg ctggcctggt ggttccagaa gcgcgaggga
      301 cagctgcgcg cgctcgattt cgccatcgcc gggctgatcc tgctgctgcc ggtgggcctg
      361 gtggtcaagc agcccgacct cggcacggcc atcctgatcc tgtcggccgg cctgtacgtg
      421 atcttcttcg cgggcctgtc gtggaagctg atcctgccgg tgctgctcct ggcggccgtc
      481 ggcatcgtgg ccgtcgtctg gagcgaggac cggatctgcc aggactccgt cgtctggccg
      541 ctgctgcgcg actaccagaa gcaccgcatc tgcacgttgc tcgaccccac caccgacccg
      601 ctcggcaagg gcttccacat catccagggc atgatcgcga tcggctcggg cggcgtcacc
      661 ggcaagggct tcatgagcgg cacgcagacc cacctcgagt tcatcccgga acgcacgacg
      721 gacttcatct tcgccgccta ctccgaggag ttcggcatgc tgggcgcgct gctgctgctg
      781 ttcggcttca cgctgctgat cttccgcggg ctggcgatcg ccgccgacgc gccgtcggtg
      841 ttcacgcggc tgctggccgg ctcgatcacg ctgagcttct tcacctacgc gttcgtgaac
      901 atgggcatgg tcagcggcat cctgcccgtg gtgggcgtgc cgctgccgtt cgtcagctac
      961 ggcggcaccg cgatggtgac gctgggcctg ggcctcgggt tcctgacctc gatcgcgcgc
     1021 aaccgccgca gcgtccagac ctgagaagct gcggacgaat cccgcacgcc ccgggcggcg
     1081 caccggattc attcacggcg cctgaacgac cggcggcctc accgcccgcc cggcgccgcc
     1141 gggccggccg cgcgggcctc gtccaccagc gcctgccagg cgaccgcccg gccgcgcgcc
     1201 acctgcacga cccgctgccg ctcgggcacc ggctgggcga gcaggtccac ggcctgcgtc
     1261 cgcggctcgt catagcgcac gcccgcggcg tcgagcagcg tcgggacgag gtccatgtgc
     1321 atcagtggcc ggcccttctg cgcggcgagc cgggcccatt gcgcggcgtg caggcgacgc
     1381 caggcgtcgt tggcccagaa gacggcgggc acgtggctgt cccagcgggt cggatagcgg
     1441 cgcgcgtggc cgacgatctg gcggccgtcg tccatcagcg cgtcgccatg gtcggacgag
     1501 tacacgagga acaccggctc gcgctgctgg cgcaggcgtg cgatgacctg gttgacgaag
     1561 ccgatggacg cgtcgaccgc gtcggcgtag gcctgccgcc tggtcgccac ggtctccttg
     1621 cgccagtgct ggccgcgggc gctgcagtcg accggatacg gcgccgtggc cgcatcgaag
     1681 cgctcgcagt acggatcgtg cgcgttgtag ccgtgcagga cgatgccctt gagcgaggcc
     1741 ggctcgcgca agctggcgtc gagcgccggc agcagccacg cgtccgtgtc gtgcgcgctg
     1801 gtggtgaagt ccacccgggc ggcgtcgggc caggcgacgt cgcggcgctg gcggttcagg
     1861 gcgatccagt gcgtcgcgaa gccggtctcg tccagggcgc gcaggaagct cccgtcgctg
     1921 gagacgcgca ccgggtgccc gggcagctcg cgcgagatca gcagcggcac cgagatgctg
     1981 gtctggtcgg cgccggcgac gacgtcgcac gcgacgagcg cgtcggcgtc cacggggcgc
     2041 accagcgccg ggccgtcgca ttccttcagg tagtcgtcgc gcacgctctc gccgacgacc
     2101 atcacgacgg tctcgcgggc ggacgcgccg ggcgcgcgcg tcgcgtgcca cgaggcggcg
     2161 gggtcgcgcg ggtcgaaggt cgacgcttcg ggatagagcc ggttcgccag ccagtgcgcg
     2221 ttcgtcgcca ccgacgcggt cgccagcgca ggctccagcg gccacagctt gaccagggtc
     2281 gtggccggca gcaccagggc cgcggccagc gtcgcgacca cggccgcgac gcgcaggcgc
     2341 cgcgtcggcg atcgcgacgc ggcgtggcgc caggcgcccc atgcgagcgc agacaccagg
     2401 accaggctgc cgagcgccat gccggcgtac ggcgacagcg cctcctccag ctcggccgac
     2461 gagtacgtgc gcagctgcag gagcaggtcc agcgcgtcga cggcgcgcag cagcgacgcg
     2521 ccgaccacca gcacgccggc cgcggcgatg ggccaagtgg cagggaagaa ggcgcgccgc
     2581 ggcagcagca accgcagcag cgccaccagc cacagcggcc cgacgatgcg ggcgacgtcg
     2641 tccagctgcc acgcgaggtc gtcgggcagc cgcgatgccg cgggcgcgac cagcagcgcg
     2701 atccacgcgg cctcgagcac gaagccgagg cccggccggc ggcccggacc ggtcctcggg
     2761 ccggacgcct cctgcacggg cgcgccgtcc tcggcgaacc aggaacgtgg ccgcatcgcg
     2821 ggcgcgggcc acgcgcgcct gtccggcgcc cgctgcgccg ggcgcgacct gcgcctcgtg
     2881 ggggcccgga cccggcggcc cggtccgctc gtcggcatgt ccgtctcttg catgcgcacc
     2941 ttgctagtaa tggggcacgt acgggcctgc ctcgcgggcc tcgtcgacga gcgtctgcca
     3001 ggaaatcgtc tcgccgagcc ggacctgaac gtcgcggcgg cgcggcggca cggcgcgggc
     3061 caggaggtcc acggggacgc ggcgcggctc gtcgtagcgc acgtcggcag cggccagcag
     3121 cgtgggcacg acgtcggcat gcatcagcgg cgcgccgacc tgcgcctgca ggttggccca
     3181 ctgcgcggca tgcgtcgcgc gccacgcgtc gttggcccag aacaccgcgg ggacctgcac
     3241 gtcccagcgc gtcggatggc gcagcccgtg gctccagatc tggcggctgt cgtcgagcag
     3301 cgcgtcaccg tggtcggagg tgtagacgaa gaagacgggc tccgggcgct ggcgcaactg
     3361 cgcgatgacc tcgtcgagga atccgaccgt ggcgtcgacc gcgttcgcgt aggacaggcg
     3421 cagccgtcgc aggctgatcg gatcgagatc ctcggccggc agcaggcaat cgacggcgta
     3481 cggcgccgtg tgcggatcga ac
//
LOCUS       DATCRO010000289         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1038103, whole genome shotgun sequence.
ACCESSION   DATCRO010000289 DATCRO010000000
VERSION     DATCRO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038103"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /gene="mutS"
                     /locus_tag="VIN75_07900"
     CDS             complement(<1..1617)
                     /gene="mutS"
                     /locus_tag="VIN75_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY8974126.1"
                     /translation="MMQQYLGLKADHPETLLFYRMGDFYEVFFDDARKANRLLDITLT
                     TRGASNGQPVVMAGVPVHALESYLARLVKLGEAVAIAEQVGEVGAGKGPVERKVVRVV
                     TPGTVTDSELLAERQETVLLAVFANRARVGLAWLALAEGRLGLGECTEAELPSWLARL
                     APAEILVSTEGAPTAVRQGTVAVVNRPAWQFDTELGLRKLYELLGVTTLAGFNAQELP
                     RAHAAAAALISYAEHTSGGARSALGHVKSLHVDKASELLELPPATLRNLELTQTLRGE
                     AAPTLLSLLDTCRTGMGSRQLRHWLTHPMRERKDATERHDAIAVLAAHGPEPLRQALR
                     GVSDVQRIVARIALRQVRPRELAGLRDTLALLPQLVQCLPPNHCRPQAAAVEPELDDL
                     GDGATSALLLRLHDALHPDPRIGAHLAAAIAAEPAVLLRDGGVIASGFDAELDELRAI
                     ATNCDDFLVALEARERERTGIGNLRVQYNKVHGFYIEVTTSGLDKVPADYQRRQTLKN
                     AERFITPELRAFEDKALSAQERSLSREKLLW"
BASE COUNT          255 a          723 c          767 g          280 t
ORIGIN      
        1 ccacagcagc ttctcgcgcg acagcgagcg ctcctgcgcc gacagcgcct tgtcctcgaa
       61 cgcgcgcagc tccggcgtga tgaagcgctc ggcgttcttg agcgtctggc ggcgctggta
      121 gtcggcgggc accttgtcca gtccggacgt ggtgacctcg atgtagaagc catgcacctt
      181 gttgtactgc acgcgcaggt tgccgatgcc ggtgcgctcg cgctcgcggg cctccagcgc
      241 gacgaggaag tcgtcgcagt tggtggcgat ggcgcgcagc tcgtcgagct cggcgtcgaa
      301 gcccgacgcg atcacgccgc cgtcgcgcag cagcaccgcg ggctcggcgg cgatggcggc
      361 ggccaggtgc gcgcctatgc gcgggtcggg atgcagcgcg tcgtgcaggc gcagcagcag
      421 ggcgctggtg gcgccgtcgc ccaggtcgtc gagctcgggt tcgacggccg cggcctgcgg
      481 gcggcagtgg ttgggcggca ggcactgcac gagctgcggc agcagcgcca gcgtgtcgcg
      541 cagcccggcg agctcgcgcg ggcgcacctg gcgcagcgcg atgcgcgcga cgatgcgctg
      601 cacgtcgctg acgccgcgca gcgcctggcg cagcggctcc gggccgtgcg cggcgagcac
      661 cgcgatggcg tcgtggcgct ccgtcgcgtc cttgcgctcg cgcatcggat gcgtcagcca
      721 gtggcgcagc tggcggctgc ccatgccggt gcggcaggtg tccagcagcg acaggagcgt
      781 gggcgccgcc tcgccgcgca gcgtctgcgt cagctcgagg ttgcgcagcg tggccggcgg
      841 cagctccagc agctcgctgg ccttgtcgac gtgcagcgac ttcacgtggc ccagcgcgct
      901 gcgggcgccg cccgaggtgt gctcggcgta gctgatcagc gccgccgcgg ccgcatgcgc
      961 gcgcggcagc tcctgcgcgt tgaacccggc cagcgtggtc acgcccagca gctcgtacag
     1021 cttgcgcagg cccagctcgg tgtcgaactg ccaggcggga cggttgacga ccgccaccgt
     1081 cccctggcgc accgccgtcg gcgcgccctc ggtcgacacc aggatctcgg ccggcgccag
     1141 gcgcgcgagc cacgacggca gctcggcctc cgtgcactcg cccaggccca gccggccttc
     1201 ggccagtgcg agccaggcca ggccgacgcg ggcgcggttc gcgaacaccg ccagcagcac
     1261 cgtctcctgg cgctcggcca gcagttccga atcggtgacg gtgcctggcg tgacgacgcg
     1321 caccaccttg cgctcgaccg gccccttgcc ggcgccgacc tcgcccacct gttcagcgat
     1381 cgccaccgcc tcgcccagct tgacgagccg cgcgaggtag gactccagcg cgtgcaccgg
     1441 aacgcccgcc atcaccaccg gctggccgtt ggacgcgccg cgcgtggtca gcgtgatgtc
     1501 cagcagccgg ttcgccttgc gggcgtcgtc gaagaacacc tcgtagaagt cgcccatgcg
     1561 gtagaacagg agcgtctcgg ggtggtcggc cttgagcccg agatactgct gcatcatcgg
     1621 cgtgtggccg ctgagggcta tcggatcttt cgaggtttcc tggggcggga ctgcagacac
     1681 gtcggggctt cacgcaaggg cgcgccgcgc gacggccgcg cggcagggag gcggattatc
     1741 gcgtggccgc gcatccggag ccgcggccgg ggcggggcgg cgatgcattg gggcattgca
     1801 cgcggaccga cgccacctcc ggcgacaagg cgccggcgca acggcgtgtc gaactcacgc
     1861 cggcgctcgg ccgggtgcat ggatcgtgca cgccggctga cccgcgcgga gagggtccgg
     1921 ccggcctgcg agggaatcga catctgcagg cggccgggtc attcggccgg cggggcgggg
     1981 tcggcggcgg tgcgggtcca gacctcgacc cggcgatcct tggcc
//
LOCUS       DATCRO010000290         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_520446, whole genome shotgun sequence.
ACCESSION   DATCRO010000290 DATCRO010000000
VERSION     DATCRO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520446"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VIN75_07905"
     CDS             complement(<1..712)
                     /locus_tag="VIN75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8974127.1"
                     /translation="MNSTATPFAIVSTAYVEGAFVEPHGHEMADLHDPSTEERIGQVR
                     LGDRADALAAIAAAKRAFPAMARTSKAERIAMLGRLHAAVSARCGDLRDAMLLEYGAP
                     RSFATFAAAHAADAFRHAADTLEAYPLERRIGTADVRMEPVGVAALITPWNSNAGFIC
                     NKLAAAIAAGCTAVVKPSEMSALQTQVVLEALHAAGLPAGVFNVVNGRGDVVGAEFAS
                     HPDVAKVSFTGSTAVGKSI"
     gene            788..1702
                     /locus_tag="VIN75_07910"
     CDS             788..1702
                     /locus_tag="VIN75_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974128.1"
                     /translation="MHRSGLIELEAVLAVARLGSFRGAAVELAMSTSAVSNAVAGLES
                     RLGVRLFNRTTRSVALSEAGDRFVAEVAPALAQIRDAMAAANDRAAAPHGTLRINTSP
                     GAALMTAPIVHEFVRRHPQMHVDIVTEGRLVDIVAAGHDAGIRLAESVPRDMVSVPMG
                     PDLGMAVVGSPAYFEHRPIPRTPSELAGHACVRTRMPSGTLARWEFTKKGRTIGVDID
                     GPLTLDDMPSTRAAALAGVALAWVSDQSVREDVERGRLVRVLADWCPSGPGLCLYYPS
                     RRHAPAGLRAFVDLIRELDPRGKAPRGR"
     gene            1777..>2002
                     /locus_tag="VIN75_07915"
     CDS             1777..>2002
                     /locus_tag="VIN75_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8974129.1"
                     /translation="MTLQRRKLGGQGLEVSAIGLGCMGMSQSYGPADEAEAIATLHRA
                     IELGCNFLDTAEVYGPLTNEALLGKALKGRR"
BASE COUNT          273 a          732 c          724 g          273 t
ORIGIN      
        1 ggatggactt gcccaccgcg gtgctaccgg tgaacgacac cttggcgacg tccggatggc
       61 tcgcgaattc cgcgcccacc acgtcgccac ggccgttgac gacgttgaac acgcccgccg
      121 gcaggcccgc ggcgtgcagc gcctcgagca cgacctgcgt ctgcagcgcg ctcatctcgc
      181 tgggcttgac gacggccgtg cagccggccg cgatcgcggc ggcgagcttg ttgcagatga
      241 agccggcgtt gctgttccac ggcgtgatca gcgcggcgac accgaccggc tccatgcgca
      301 cgtcggccgt gccgatgcgg cgttccagcg gataggcctc cagcgtgtcg gccgcgtgcc
      361 tgaacgcgtc tgcggcgtgc gcggcggcga aggtcgcgaa gctgcgcggg gcgccgtact
      421 cgagcagcat cgcgtcgcga aggtcgccgc aacgcgcgct cacggccgcg tgcagccgcc
      481 cgagcatcgc gatgcgctcg gccttggacg tccgggccat cgccgggaac gcgcgcttcg
      541 cggcggcgat cgcggccagc gcgtccgccc ggtcgccgag gcgtacctgg ccgatgcgct
      601 cctcggtgga cgggtcgtgc aggtcggcca tctcgtggcc gtgcggctcg acgaacgcgc
      661 cctcgacgta ggccgtggac acgatggcga agggggtggc ggtggagttc atggcgccag
      721 tctgcgcgcc ggccgtcgat ttgataagcc acacgattcg cgacgattca ttgcgataat
      781 cgggacaatg caccgaagcg gactgatcga actggaggcc gtgctcgccg tcgcgcggct
      841 cggcagcttc cgcggcgcgg ccgtcgagct ggcgatgtcc acgtcggcag tgagcaacgc
      901 cgtcgccggg ctggagtcgc gcctgggcgt gcggctgttc aaccgcacca cgcgcagcgt
      961 cgcgctgtcg gaggccggcg atcgcttcgt ggccgaggtc gcgccggcgc tcgcgcagat
     1021 ccgcgacgcg atggccgccg ccaacgaccg ggcggccgcg ccgcacggca cgctgcgcat
     1081 caacacgagc cccggcgccg cgctgatgac ggcgcccatc gtccacgagt tcgtgcgccg
     1141 tcacccgcag atgcacgtcg acatcgtgac cgagggccgg ctggtcgaca tcgtcgcggc
     1201 cggccacgac gccggcatcc gcctggccga gagcgtgccg cgcgacatgg tctcggtgcc
     1261 gatgggcccc gatctcggca tggccgtcgt cggctcgccg gcctacttcg agcaccgccc
     1321 gatcccgcgg acgccgtccg agctcgccgg ccacgcctgc gtgcgcacgc gcatgcccag
     1381 cggcacgctg gcgcgctggg agttcacgaa gaaaggccgg acgatcggcg tcgacatcga
     1441 cggcccgctg acgctcgacg acatgcccag cacgcgcgcc gccgcgctcg cgggggtggc
     1501 gctggcgtgg gtctccgacc agtcggtgcg cgaggacgtc gaacgcggcc gcctcgtgcg
     1561 cgtgctggcc gactggtgcc cgtccggccc gggcctgtgc ctgtactacc ccagccgccg
     1621 ccatgccccc gcgggcctgc gcgccttcgt cgacctgatc cgcgagctcg acccgcgcgg
     1681 caaggcccca cgcggacgtt gacatccgcc gcggtcgctg ccggccccgc gcgttcgaca
     1741 tagagtcgac ggcaaaccct tcatgacgga gcgacgatga cactgcaacg acgcaagctg
     1801 ggcggccagg ggctggaggt ctcggccatc ggcctgggct gcatgggcat gagccagtcc
     1861 tacgggccgg ccgacgaggc cgaggccatc gccacgctgc accgggcgat cgagctgggc
     1921 tgcaacttcc tggacaccgc cgaggtctat ggcccgctga ccaacgaggc cctgctgggc
     1981 aaggcgctca aggggcggcg cc
//
LOCUS       DATCRO010000291         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_655841, whole genome shotgun sequence.
ACCESSION   DATCRO010000291 DATCRO010000000
VERSION     DATCRO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655841"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VIN75_07920"
     CDS             complement(<1..296)
                     /locus_tag="VIN75_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8974130.1"
                     /translation="MPTAAPGAVEGGRFNVSRWALQHPELTRYLMIVLMLLGLAAYFQ
                     LGQDEDPPFTFRAMVVQAYWPGASAKDVAEQVTDKIERTLQEVPYSDKIRSYTKP"
     gene            complement(322..1452)
                     /locus_tag="VIN75_07925"
     CDS             complement(322..1452)
                     /locus_tag="VIN75_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8974131.1"
                     /translation="MNTPLAPNRPPARRALTALPLALSLAASVVLVACGKEPAAPEPV
                     RAVRTMTVGQLSGATVRDYAGEVHARIESRLAFRVGGKIVQRPVNLGDRVKGGQALAE
                     VDPADLRFGQDAARAGLAAAKVSYEQTAADYKRYEDLRAQGFISAAELERRNSGLEAA
                     KAQFDQARAQAGLQTNQASYAALTADAPGVITAVFADVGAVVGAGSPVVSLAHDGPRD
                     VVFAVPEDQLAIFRKLQGKPGGVSVTVWGGNATIPATVREVAAAADPASRTFQVKADL
                     PAGAVELGQTATVHVELAPADGKLRLPLQAVAGSGNESHVWVLDKATMKVREQPVTVL
                     RPEGDALVIDGGLKSGDVVVTAGAHVLSPNQQVTLYVEPAKR"
     gene            1563..2468
                     /gene="hpnC"
                     /locus_tag="VIN75_07930"
     CDS             1563..2468
                     /gene="hpnC"
                     /locus_tag="VIN75_07930"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY8974132.1"
                     /translation="MASPGPSPRRTRRAQSRAVSVDHYENFPVASWLCPPALRPAVRA
                     IYWFARTADDIADEGDAPAAARQADLAAYRQDLLAALAGQPPSARWPAIFGPLAQVIA
                     AHHLPASELLALLDAFEEDTRHAGYADRAALVQYCSRSANPVGRLLLHLHGLADPELQ
                     RRSDAVCTALQLVNFWQDLSVDLPRGRDYVPRSDAAAHATTSMDCLREPHAHASRALL
                     RELCAWARELMLEGAPIAPAVPGRVGWELRLVVQGGLRILAKIEAMNFASFARRPRLH
                     AADHLLIGLRALTMRPSLPERSRIA"
     gene            2465..3313
                     /gene="hpnD"
                     /locus_tag="VIN75_07935"
     CDS             2465..3313
                     /gene="hpnD"
                     /locus_tag="VIN75_07935"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY8974133.1"
                     /translation="MTPEQYVQDKAQKSGSSFYYAFMFLPPARRQAMTAFYAFCREVD
                     DVVDEVQDPGVAATKLDWWRREVDASFAGNPSHPVMKALMPHVPLYGVRVDHLHAVIQ
                     GCQTDLEQTRFLDYPGLQRYCHQVAGIVGEVASNIMGRTQGQTVKFAHQLGLAMQLTN
                     IIRDVGDDARRGRIYLPISELQQFDVKANEILKREAPWGYSERFTALMKFQAARAHAL
                     FDEALALLPDEDRYAQRPALMVANIYRRLLREVEAAGFQVLHQRISLTPLRKLWIACQ
                     TQWRGR"
     gene            3561..>3780
                     /locus_tag="VIN75_07940"
     CDS             3561..>3780
                     /locus_tag="VIN75_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY8974134.1"
                     /translation="MRLAVIGGGWAGLAAAVRATQAGHAVTLFEAAAMPGGRARSDDA
                     GDGATDNGQHILLGGYGRTLDLMRTVGVD"
BASE COUNT          516 a         1402 c         1320 g          542 t
ORIGIN      
        1 ggcttggtgt agctgcggat cttgtcggag tacggcacct cctgcagcgt gcgctcgatc
       61 ttgtcggtca cctgctcggc gacgtccttg gcgctcgcgc ccggccagta ggcctgcacg
      121 accatcgcgc ggaacgtgaa cggcggatcc tcgtcctggc cgagctggaa gtaggccgcc
      181 aggcccagca gcatcagcac gatcatcagg tagcgcgtga gttccgggtg ctgcagcgcc
      241 cagcgcgaga cgttgaagcg tccgccctcg accgcgcccg gagcggcggt cggcatcgtt
      301 tgttcgttga tcacgcagac ctcagcgctt ggcgggttcg acgtacaggg tgacctgctg
      361 gttcggcgac agcacgtgcg cgccggcggt gacgacgacg tcgccgctct tgagcccgcc
      421 gtcgatcacc agcgcatcgc cttcgggccg cagcacggtc acgggctgct cgcgcacctt
      481 catcgtggcc ttgtccagca cccacacgtg gctctcgttg ccgctcccgg ccacggcctg
      541 cagcggcagg cgcagcttgc cgtcggccgg cgcgagctcg acgtgcaccg tcgccgtctg
      601 ccccagctcg accgcgcccg cgggcaggtc ggccttgacc tggaacgtgc gcgaggccgg
      661 atccgcggcg gcggccacct cgcgaaccgt cgcggggatc gtcgcgttgc cgccccacac
      721 ggtgacgctg acgccgccgg gcttgccctg cagcttgcgg aagatcgcga gctggtcctc
      781 gggcaccgcg aacaccacgt cgcgcgggcc gtcgtgcgcg aggctcacca ccggcgagcc
      841 ggcgcccacc accgcgccga cgtcggcgaa gacggccgtg atcacacccg gggcgtcggc
      901 ggtcagcgcg gcgtagctgg cctggttggt ctgcaggccg gcctgcgcgc gcgcctggtc
      961 gaactgcgcc ttcgcggcct ccagcccgct gttgcggcgc tcgagctcgg cggcactgat
     1021 gaagccctgg gcgcgcaggt cctcgtagcg cttgtagtcg gcggccgtct gctcgtagct
     1081 caccttggcg gcggccagcc cggcgcgcgc ggcgtcctgt ccgaagcgca ggtcggccgg
     1141 atcgacctcc gccagcgcct ggccgccctt gacgcgatcg ccgaggttga cggggcgctg
     1201 cacgatcttg ccgccgaccc ggaacgccag gcgcgactcg atgcgggcgt gcacctcgcc
     1261 ggcgtagtcg cgcaccgtgg cgcctgacag ctggccgacg gtcatggtgc gcaccgcgcg
     1321 caccggttcg ggggcggccg gctccttgcc gcaggccacc agcaccacgc tggcggccag
     1381 ggacagcgcg agaggcagcg ccgtgagggc gcggcgggcc gggggacgat tcggggcgag
     1441 gggggtgttc atgggtgttc gatctactta ctgaccagtc agtcagtaat gtacgaacag
     1501 cgccgcgact cgtcaatgga cctgtccggc ggaaacaagg gaaaaccggg aattcagggc
     1561 ggatggcgtc ccccggcccc tcgccccgcc gcacgcggcg ggcacaatcg cgcgccgtga
     1621 gcgtcgatca ttacgagaac ttccccgtcg catcgtggct gtgcccgccg gcgctgcgtc
     1681 cggccgtgcg cgccatctac tggttcgcgc gcaccgcgga cgacatcgcc gacgaaggcg
     1741 acgccccggc cgccgcgcgc caagccgatc tcgcggccta tcggcaagac ctgctcgccg
     1801 cgctggccgg ccagccaccg tcggcgcgtt ggccggcgat cttcggcccg ctcgcgcaag
     1861 tgatcgccgc gcaccatctg cccgcgtcgg agctgttggc gctgctcgac gccttcgagg
     1921 aggacacgcg ccacgccggc tatgccgacc gcgccgccct tgtgcagtac tgcagccgct
     1981 cggccaatcc ggtcggccgc ctgctgctgc acctgcacgg cctcgccgac cccgaactgc
     2041 agcgccgcag cgacgccgtc tgcaccgcgc tgcaactggt caacttctgg caggacctga
     2101 gcgtcgatct gccgcgcggc cgcgactacg tgccgcgcag cgacgccgcc gcgcacgcga
     2161 cgacgtccat ggactgcctg cgcgaaccgc acgcgcacgc gtcgcgtgcg ctcctgcgcg
     2221 agctgtgcgc atgggcgcgc gagctgatgc tcgagggtgc gcccattgcg ccggcggtgc
     2281 ccggccgcgt gggctgggag ctgcgactcg tcgtccaggg cgggctgaga attcttgcga
     2341 aaatcgaggc catgaacttc gcgagctttg cccgccgtcc ccgactccac gccgccgatc
     2401 acctgctgat cggccttcgc gccctgacga tgcgcccttc cctcccagaa cgatcgcgca
     2461 tcgcatgacc cccgagcaat acgtccagga caaggcccag aagagcggct cgtcgttcta
     2521 ctacgccttc atgttcctgc cgcccgcgcg gcgccaggcg atgacggcct tctacgcgtt
     2581 ctgccgcgag gtcgacgatg tcgtcgacga ggtgcaggac ccgggcgtcg ccgccaccaa
     2641 gctcgactgg tggcgccgcg aggtcgacgc gtccttcgcc ggcaacccgt cgcaccccgt
     2701 gatgaaggcg ctgatgccgc acgtgccgct gtacggcgtg cgcgtggatc acctgcacgc
     2761 cgtgatccag ggctgccaga ccgacctcga gcagacgcgc ttcctcgact accccggcct
     2821 gcagcgctac tgccaccagg tggccggcat cgtcggcgag gtcgccagca acatcatggg
     2881 ccgcacgcag ggccagaccg tgaagttcgc gcaccagctg ggcctggcga tgcagctcac
     2941 caacatcatc cgcgacgtcg gcgacgacgc gcgccgcggc cgcatctacc tgccgatctc
     3001 cgagctgcag cagttcgacg tcaaggccaa cgagatcctc aagcgcgagg cgccgtgggg
     3061 ctacagcgag cgcttcaccg cgctgatgaa gttccaggcc gcgcgcgccc acgcgctgtt
     3121 cgacgaggcg ctggcgctgc tgcccgacga ggaccgctac gcccagcgcc ccgcgctgat
     3181 ggtcgccaac atctatcgcc gcttgctgcg cgaggtcgag gccgccggct tccaggtgct
     3241 gcaccagcgc atctcgctca caccactgcg caagctgtgg atcgcatgcc agacgcagtg
     3301 gcggggccgc tgaaggcggc catcgcaccc gcttgcgagg cggggccgct gaaggcggcc
     3361 atcgcacccg cttgcggggc ggggccgctg aaggcggcca tcgcggccgc ttgcggggcg
     3421 gggccgctga aggcggccat cgcggccgct tgcagggcgg ggccgctgaa ggcggccatc
     3481 gcggccgctt gcaggccggg gccgctgaag gcggccatcg cacccgcttg cggggcgctg
     3541 ccgctgaagc cagtcaggcc atgagactcg ccgtcatcgg cggaggatgg gccggcctcg
     3601 cggcggccgt ccgcgcgacg caggcgggcc atgccgtcac gctcttcgag gccgcggcca
     3661 tgcccggcgg tcgtgcgcgc tcggacgacg cgggcgacgg cgcgaccgac aacggccagc
     3721 acatcctcct gggcggctac ggccgcacgc tggacctgat gcgcaccgtg ggcgtcgacc
//
LOCUS       DATCRO010000292         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_756591, whole genome shotgun sequence.
ACCESSION   DATCRO010000292 DATCRO010000000
VERSION     DATCRO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756591"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1287
                     /locus_tag="VIN75_07945"
     CDS             136..1287
                     /locus_tag="VIN75_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974135.1"
                     /translation="MKDLPGHAPPMLLERLKAVITLALGIALAGPAHAQLIIGQTTGV
                     TGNSAVNVAETTLGAKLWIDAVNARGGVNGNLVELRTLDDQGSATQAAANAKTLIERD
                     QVLALFMIRGTPQNEAVMPLLDRYDIASIAPSSGALLLSAPVNRHVFNVRNSYQAEAE
                     RAVEQLASMGSTRIAVLKTDDAFGDDSLAGATRGFARMQLEPVFVEGFDKAAPKFDGV
                     AARLTRSNAQAVIVLGTASSVVKATRAIRAAGNSARVVTLSNNASEGLVRELGPLARG
                     VIVTQVFPGENEITLPMIRDATRLLQAREPGARPSPAMIEGFAAARVLVEALRRAGPH
                     PTRRSVIAALDSMDDFDLGGVKVHYRESDHSGVRYADLSVIDERGRFRR"
     gene            1334..2362
                     /locus_tag="VIN75_07950"
     CDS             1334..2362
                     /locus_tag="VIN75_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8974136.1"
                     /translation="MHKIPATLIAGDGIGPEIVDATVTVLDALGAPFDWDRQAAGLAG
                     VAAHGDPLPDVTLQSIRRTRLALKGPLETPTGGGFRSSNVRLREEFKLYANLRPAVTL
                     VPGGRFEDIDLVVVRENLEGLYIGHEHFVAIDDDPHAVAIATGINTRAGSRRLLKFAF
                     DQAVAMRRRKVTVVHKANIMKALTGIFLETARDLYEGDYKGRFEFDTVIVDACAMKLV
                     TNPWQFDMLVTTNLFGDILSDLVAGLVGGLGMAPGANIGADAAIFEAVHGSAPDIAGR
                     DIANPTALILAAGLMLDHVGLREQASRLRRALSDTLVVDSIRTRDLGGTARIQGFTQA
                     LVRRIQAA"
     gene            complement(2400..2747)
                     /locus_tag="VIN75_07955"
     CDS             complement(2400..2747)
                     /locus_tag="VIN75_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974137.1"
                     /translation="MVFSGERRVHDFEKLEQQWEFWPEATALCRRKGIRRVLVLNELV
                     GEISSTYVRGLHLNLEEFGLDQGLRYAMVVGEPRARGVLTFGIELAKGLGWDIEIFAE
                     TAPARAWLARPFP"
     gene            complement(2977..>3459)
                     /locus_tag="VIN75_07960"
     CDS             complement(2977..>3459)
                     /locus_tag="VIN75_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8974138.1"
                     /translation="SEVWSSGHRTPYGLAFAPDGRLWELEHGPRGGDELNLIEPGRNY
                     GWPLVSYAVNYDGVPIASPDTRPDLAKPVIYWTPVLAPGSLMFYSGAMFPKWKGSAFA
                     GGLVSHALHRIEVHGATATPAEHWTVGFRLRDVAQAPDGALWLIEDAHDGGLYRLTPK
                     "
BASE COUNT          538 a         1261 c         1169 g          491 t
ORIGIN      
        1 cccatcaccc ccgcctcccg acccgggatg cgtgtccgtc agtcaatcac cgccacgacg
       61 gtgagcgcgg gcgcgacgcc acgcgcacgg acgcaggccg tctgccgccg gcgacgtcaa
      121 ccaacggaga cgaccatgaa ggaccttccc ggtcacgcac cgcccatgct cctggagcgc
      181 ttgaaggcgg tcatcacgct cgccctgggc atcgccctcg ccggcccggc ccacgcccag
      241 ttgatcatcg gccagacgac gggcgtcacc ggcaattcgg ccgtcaacgt ggccgagacg
      301 acgctgggcg cgaagctctg gatcgacgcc gtcaatgcgc gcggcggcgt gaacggcaat
      361 ctcgtcgagc tgcgcacgct ggacgaccag ggctccgcga cgcaggccgc ggccaatgcg
      421 aagacgctga tcgagcggga ccaggtgctc gcgctgttca tgatccgcgg cacgccgcag
      481 aacgaggcgg tgatgcccct gctggatcgc tacgacatcg cgtcgatcgc gccgtccagc
      541 ggcgccctgc tgctcagcgc cccggtcaac cggcatgtct tcaacgtgcg caactcctac
      601 caggccgagg cggagcgcgc cgtcgaacag ctcgccagca tgggcagcac gcgcatcgcc
      661 gtgctcaaga ccgacgacgc cttcggcgac gactcgctgg ccggcgcgac ccggggcttt
      721 gcgaggatgc agctcgaacc ggtgttcgtc gaaggcttcg acaaggccgc tccgaagttc
      781 gacggcgtcg cggcgcggct gacgcgatcg aacgcgcagg ccgtcatcgt cctgggcacg
      841 gcctcttcgg tggtcaaggc gacgcgcgcg atccgggcgg cgggcaacag cgcgagggtc
      901 gtgaccttgt cgaacaacgc ctcggagggc ctggtccgcg aactcggtcc gctcgcgcgc
      961 ggcgtcatcg tcacgcaggt ctttcccggc gagaacgaga tcaccctgcc gatgatccgc
     1021 gacgccacgc ggctcctgca ggcccgcgag cccggcgccc gcccgtcgcc cgcgatgatc
     1081 gaaggcttcg ccgcggcgcg cgtgctggtg gaggccctcc gacgcgcggg cccccatccg
     1141 acgcgcagga gcgtcatcgc ggccctcgac tccatggacg acttcgacct gggcggcgtc
     1201 aaggtgcact accgcgaatc ggaccattcc ggcgtgcgct acgccgactt gagcgtcatc
     1261 gacgaacgcg gccggttccg ccgctgatgc gaccccggcc catcgacttc cccccaacga
     1321 ctccccgacc ccgatgcaca agatccctgc caccctcatc gccggcgacg gcatcggccc
     1381 cgaaatcgtc gacgccaccg tgaccgtgct cgacgcgctc ggcgccccct tcgactggga
     1441 tcgccaggcc gccggcctgg ccggcgtggc cgcccacggc gatccgctgc ccgacgtgac
     1501 gctgcagagc atccggcgca cgcgcctggc cctcaaggga ccgctggaga cgccgacggg
     1561 cggcggattc cggtcgtcca acgtccgcct tcgcgaggag ttcaagctct acgccaacct
     1621 gcgcccggcg gttacgctcg tgcccggcgg ccgcttcgag gacatcgacc tcgtggtcgt
     1681 ccgggagaac ctcgaggggc tctacatcgg gcacgagcac ttcgtggcga tcgacgacga
     1741 tccgcacgcc gtggccattg ccaccgggat caacacgcgc gccggcagcc gccggctcct
     1801 gaagttcgcc ttcgaccagg cggtggccat gcgccgccgg aaggtcaccg tcgtccacaa
     1861 ggccaacatc atgaaggccc tgacgggcat cttcctggag acggcgcgcg acctctacga
     1921 gggcgactac aagggccggt tcgagttcga cacggtcatc gtcgacgcct gcgcgatgaa
     1981 gctggtcacc aacccgtggc agttcgacat gctggtcacg accaacctgt tcggcgacat
     2041 cctgtcggac ctggtcgccg ggctcgtcgg ggggctgggc atggcgccgg gcgcgaacat
     2101 cggcgcggat gcggcgatct tcgaggccgt gcacggatcc gcgcccgaca tcgccgggcg
     2161 cgacatcgcg aatccgaccg cgctgatcct ggcggccggc ctgatgctgg accacgtggg
     2221 cctgcgcgaa caggccagcc ggcttcgccg cgcgctgagc gacacgctgg tcgtcgactc
     2281 gatccggacg cgggacctgg gcggcacggc gcggatccag ggcttcacgc aggcgctggt
     2341 gcggcgcatc caggccgcct gaggccgcgc ggacgggcga ctcgctgcct gcggcgggct
     2401 catggaaacg gtcgcgccag ccaggctcgc gcgggggcgg tctccgcgaa gatctcgatg
     2461 tcccagccca ggcccttcgc gagctcgatg ccgaaggtca gcacgccccg ggcgcgcggc
     2521 tcgccgacca ccatcgcgta gcgcaggccc tggtcgaggc cgaactcctc gaggttcagg
     2581 tgaagtccgc gcacataggt gctggagatc tccccgacca gttcgttgag cacgagcacg
     2641 cgacgaatgc ccttgcgccg gcaaagcgcc gtcgcctcag gccagaactc ccattgctgt
     2701 tcgagcttct cgaaatcgtg gacacggcgt tcgccgctga agaccaccgt caaggtgtct
     2761 ccagtctcgg aaatggagaa gttcgatcca gccgtggcag cctccgtctg cggctcgccg
     2821 catgcgcggt gcgtgcccag tttggccgat gtcgctcgag atggacatgg cccgcgtcaa
     2881 atgcatccgt gcagcgtgag gcattcgaca cacccgatgc cgtgcgccgc aaggcgcgtt
     2941 ccccggcagc gcgacgttcg acagcaccgt cccgcgtcac ttcggcgtga gccggtacaa
     3001 cccgccgtcg tgggcgtcct cgatcaacca cagcgcgccg tccggcgcct gcgcgacgtc
     3061 gcgcagccgg aagccgacgg tccagtgttc ggccggcgtc gcggtggcgc cgtgcacctc
     3121 gatgcggtgc agcgcgtggc tcacgaggcc accggcgaac gccgaaccct tccacttcgg
     3181 gaacatcgcg ccgctgtaga acatgaggct gccgggcgcg agcaccggcg tccagtagat
     3241 gacgggcttg gcgaggtcgg gccgcgtgtc ggggctcgcg atgggcacgc cgtcgtagtt
     3301 cacggcatac gagacgagcg gccagccgta gttcctgcct ggctcgatca ggttgagctc
     3361 gtcgccaccg cgcggcccgt gctccagctc ccacaggcgg ccgtcgggcg cgaaggcgag
     3421 gccgtagggg gtgcgatggc cggaggacca cacttccga
//
LOCUS       DATCRO010000293         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_239324, whole genome shotgun sequence.
ACCESSION   DATCRO010000293 DATCRO010000000
VERSION     DATCRO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239324"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1000
                     /locus_tag="VIN75_07965"
     CDS             134..1000
                     /locus_tag="VIN75_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY8974139.1"
                     /translation="MSDIPNFAKLVPGFDFLQGLVKNAGSALPSMGQWVAPTLNPEEL
                     QKRIDELKTVQFWLEQNARMLGTTIQALEVQRMTLSTLKTMNVQMTDLRDAMKVAPVA
                     SAGSAAAAAAGSVASSLGADALMQAARTAMGGIPGLGKAAAPAPAAAEEPEPAPAPKP
                     KAARPRPAPAEPPAVAATGVVDPMQWWGALTKQFTQLAASAMKDSATDAAKNLAGAMM
                     KQGMDAAGETFKKAVAVPAGVAAKAMSAATQAAGATGARAANGKAAAAKKAPARKTPA
                     RKTTRAAKPRAS"
     gene            990..>2378
                     /locus_tag="VIN75_07970"
     CDS             990..>2378
                     /locus_tag="VIN75_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8974140.1"
                     /translation="MRVEPTTIVDTMPMPADPGWASSAPASVPVSAPPHGREPAGAPD
                     ASAPEPARPAVSQQALVDALRGIVPAHALLWRGEETTPYECDGLTAYRERPLAVALPE
                     TEEQVAAILSACHRLGVPVVARGAGTGLSGGAMPHAHGLTLSLARFNRIVKVDARART
                     ATVQSGVRNLAISEAVAPHGFYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTLHNV
                     LQVRGFLADGSPATFGSLALDAPGLDLMSVVVGSEGMLAVVTEVVVKLTPKPQVARCI
                     MASFSDVRHAGDAVAAIIADGLIPAGLEMMDKPMTAAVEAFVHAGYDLDAAAILLCES
                     DGTPEEVEEEIGRMSEVLRAAGATAIAVSRDEAERLRFWSGRKNAFPASGRISPDYMC
                     MDSTIPRKRLADILLAIQEMEKKYQLRCANVFHAGDGNLHPLILFDANDPDQLHRAEL
                     FGADILETSVALG"
BASE COUNT          358 a          878 c          857 g          285 t
ORIGIN      
        1 ggtcgcgtcg ggctgcccac gctgctgagg gcgcgcggct tccaggtcga gcgcgtgccg
       61 gtcgccgacg cgcgctgaag atgcggccgt gaaagcgtag gatcatccac ctgccgccac
      121 cgacgaggtt cccatgtccg acatcccgaa tttcgccaag ctcgtgcccg gcttcgactt
      181 cctgcagggc ctggtcaaga acgccggctc cgcgttgccg agcatggggc agtgggtggc
      241 cccgacgctc aaccccgagg agctgcagaa gcgcatcgac gagctcaaga cggtgcagtt
      301 ctggctcgag cagaacgcca ggatgctcgg gacgacgatc caggcgctcg aggtgcagcg
      361 catgacgctg tcgacgctca agacgatgaa cgtgcagatg accgacctgc gcgacgcgat
      421 gaaggtggcg cccgtcgctt cggccggctc cgccgcggcg gccgccgccg ggtcggtggc
      481 cagcagcctg ggcgccgatg cgctgatgca ggccgcgcgc accgcgatgg gcggcatccc
      541 cggcctgggc aaggcggccg ccccggcgcc cgccgccgcg gaggaacccg agccggcgcc
      601 cgcgcccaag cccaaggccg ccaggcccag gcccgcgccg gccgagccgc cggccgtcgc
      661 cgccaccggt gtcgtcgacc cgatgcagtg gtggggcgcg ctgaccaagc agttcacgca
      721 gctggccgcc agcgcgatga aggacagcgc caccgacgcc gccaagaacc tggccggcgc
      781 gatgatgaag cagggcatgg acgccgccgg cgagaccttc aagaaggccg tggcggtgcc
      841 ggccggcgtg gccgccaagg cgatgtcggc cgccacccag gcggccggcg ccaccggggc
      901 gcgcgccgcc aacggcaagg ccgcggccgc caagaaggcg ccggcgcgca agaccccggc
      961 ccgcaagacc acccgagccg ccaagccccg tgcgagttga accgaccacg atcgtcgaca
     1021 cgatgcccat gcccgccgac cccggctggg ccagttcggc gccggccagc gtgccggtgt
     1081 cggcgccgcc gcacgggcgc gagcccgcgg gcgcgcctga cgcgtccgcg cccgagcccg
     1141 cgcggccggc ggtgagccag caggcgctgg tcgacgcact gcgcggcatc gtgccggcgc
     1201 acgcgctgct gtggcgcggc gaggagacga cgccctacga gtgcgacggc ctgaccgcgt
     1261 accgcgagcg tccgctggcc gtggcgctgc ccgagacgga ggaacaggtc gccgcgatcc
     1321 tgtccgcctg ccatcgcctg ggcgtgccgg tggtggcgcg cggcgcgggc accgggctgt
     1381 ccggcggcgc gatgccgcat gcgcacgggc tcacgctgtc actggccagg ttcaaccgca
     1441 tcgtcaaggt cgacgcccgg gcccgcaccg ccaccgtgca gtcgggcgtg cgcaacctgg
     1501 ccatcagcga ggccgtcgcg ccgcacggct tctactacgc gcccgatccg tccagccaga
     1561 tcgcgtgcac catcggcggc aacgtggcgg agaactcggg cggcgtgcac tgcctgaagt
     1621 acggcctgac gctgcacaac gtgctgcagg tgcgcggctt cctggctgac ggcagcccgg
     1681 cgaccttcgg ctcgctggcg ctcgacgcgc cggggctgga cctgatgtcg gtggtggtcg
     1741 gcagcgaggg catgctggcc gtcgtcaccg aggtcgtcgt caagctcacg cccaagcccc
     1801 aggtggcgcg ctgcatcatg gccagcttct cggacgtgcg ccacgcgggc gacgcggtcg
     1861 cggccatcat cgcggacggt ctgatccccg ccggcctcga gatgatggac aagccgatga
     1921 cggccgccgt cgaggccttc gtgcatgccg gctacgacct cgacgccgcc gccatcctgc
     1981 tgtgcgagag cgacggcacg cccgaggaag tcgaggaaga gatagggcgc atgagcgagg
     2041 tcctgcgtgc ggcgggcgcc accgccatcg ccgtgagccg cgacgaggcc gagcgcctgc
     2101 ggttctggag cggccgcaag aacgccttcc cggcctccgg tcgcatcagc cccgactaca
     2161 tgtgcatgga ctccaccatc ccgcgcaagc ggctggcgga catcctgctg gccatccagg
     2221 agatggagaa gaagtaccag ctacgctgcg cgaacgtctt ccacgcgggc gacggcaacc
     2281 tgcacccgct gatcctgttc gacgcgaacg atccggacca gctgcaccgc gccgagctgt
     2341 tcggcgccga catcctggag accagcgttg cgctcggc
//
LOCUS       DATCRO010000294         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_374253, whole genome shotgun sequence.
ACCESSION   DATCRO010000294 DATCRO010000000
VERSION     DATCRO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374253"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1214
                     /locus_tag="VIN75_07975"
     CDS             282..1214
                     /locus_tag="VIN75_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8974141.1"
                     /translation="MSAIVDSFFPLLALILLGWILRRRDLLGAAAARELTAFVVWLAL
                     PSLLFEGIAGASWQQLWQPRFVLAYAGAMVATFLLLFWWLPRTLPFAERCLDCFGATY
                     ANSGFIGIPLSVLLLGKEALIPATIATLLTAVLLFAVGIVLLELGAGSPTRPLATVGK
                     AAHAVARNPLVVAAVAGGLWSAGGWTMPQAPHRLLELLAVAAGPCALVSIGALLAQPA
                     PPGSAATPLAPVVVAKLVVLPAICALLAIEVFALPPPWATTAVLLSALPAGTGPFMLA
                     EFHGRDVARASRIMFVTTILSLASVPACIALLRC"
     gene            complement(1251..1925)
                     /locus_tag="VIN75_07980"
     CDS             complement(1251..1925)
                     /locus_tag="VIN75_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974142.1"
                     /translation="MSIDDSFAFGHDEIFLAWIAGWARSRDTPPPVAHADGFRIEVGL
                     PQQAARFVFPHLSPTLRELGATIAQPWVLLKACGTSAQLRALLPAHWRIEDQHWMMTC
                     GARRFTGDAALPAGYSLQVDVDTARSGRAHVLVVAPDGVQAAAGHVGLDGPLAIYDRI
                     VTDPAHQRRGLGRAVMHALQALAHAHGLRAGALVATDQGRALYESLGWRLHAPWAGAV
                     IPGPAA"
     gene            complement(1958..2224)
                     /locus_tag="VIN75_07985"
     CDS             complement(1958..2224)
                     /locus_tag="VIN75_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974143.1"
                     /translation="MDWHCIKFDRRGAHESEAAELVLALEDAYGSAGEPPSAEVFMAH
                     GRVGDYAFYLSPEASRMAPAVLQRFHAVLCEPPVDLHRCTPMIL"
     gene            2311..2398
                     /locus_tag="VIN75_07990"
     tRNA            2311..2398
                     /locus_tag="VIN75_07990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2346..2348,aa:Leu,seq:taa)
BASE COUNT          332 a         1096 c         1072 g          399 t
ORIGIN      
        1 gctggcgggg ctggcggtgg tcgcgctgct cgcgctgctc gcgctggggc cgctgcggcg
       61 ccgcgtgcgc gccggcctcg cgcggcgcgg cgcgtcgggg gcggctggcg tgggggagca
      121 cgcggccgcc gccaacgact cgcgccagga cctggccgcc tgagcgcgcc ggttcacact
      181 gtggcgcgaa gccgcggccg gcgatggccg cgcgcaggtc gccgccgcgg gccctgtggt
      241 ggaatcgact gtcctcgcgc cgccggggcc acgcgcccgc catgtccgcc atcgtcgaca
      301 gcttctttcc gctcctcgcg ctgatcctgc tcgggtggat cctgcgccgc cgcgacctgc
      361 tgggcgccgc cgccgcacgc gagctcaccg cattcgtcgt ctggctcgcg ctgccgtcgc
      421 tgctgttcga gggcatcgcc ggcgcgtcgt ggcagcagct gtggcagccg cgcttcgtgc
      481 tggcctacgc gggcgccatg gtggccacgt tcctgctgct gttctggtgg ctgccgcgca
      541 cgctcccgtt cgccgagcgc tgtctcgact gcttcggcgc cacttatgcg aactcgggct
      601 tcatcggcat cccgctgagc gtgctgctgc tgggcaagga ggcgctgatc cccgcgacga
      661 tcgccacgct gctgacggcc gtgctgctgt tcgccgtcgg catcgtgctg ctcgagctcg
      721 gcgcgggctc gcccacgcgg ccgctcgcca cggtgggcaa ggcggcgcat gcggtggcgc
      781 gcaatccgct cgtcgtcgcc gccgtcgccg gcggcttgtg gtcggccggc ggctggacga
      841 tgccgcaggc gccgcaccgg ctgctcgaac tgctggcggt ggcggccggg ccgtgcgcgc
      901 tggtgtcgat cggcgcgctg ctcgcgcaac ccgcgccgcc gggcagcgcc gcgacgccgc
      961 tcgcgccggt ggtcgtcgcc aagctggtcg tgctgcctgc gatctgcgcg ctgctggcga
     1021 tcgaggtgtt cgcgctgccg ccgccgtggg cgacgaccgc cgtgctgctg agcgcgctgc
     1081 ccgcgggcac cggcccgttc atgctggccg agttccatgg gcgcgacgtg gcgcgcgcgt
     1141 cgcgcatcat gttcgtgacg acgatcctgt cgctggccag cgtgccggcg tgcatcgcgc
     1201 tgctgcgctg ctgaccggcc gtcgcgtcgc cgcgcgaccg cggctgtcgt tcatgcggcc
     1261 ggtccgggga tgacggcgcc ggcccagggc gcatgcaggc gccatcccag cgattcgtac
     1321 agcgcccggc cctggtcggt ggccacgagc gccccggcgc gcagcccgtg cgcgtgcgcg
     1381 agcgcctgca gcgcgtgcat gacggcgcgc ccgaggccgc gccgctgatg cgccgggtcg
     1441 gtgacgatgc ggtcgtagat cgcgagcgga ccgtccaggc cgacgtgccc ggccgcggcc
     1501 tggacgccgt cgggcgcgac gaccagcacg tgcgcgcgcc cgctgcgggc cgtgtcgacg
     1561 tcgacctgga gcgagtagcc cgccggcagc gcggcgtcgc cggtgaaccg gcgcgcgcca
     1621 caggtcatca tccagtgctg gtcctcgatg cgccagtgcg cgggcagcag ggcgcgcaac
     1681 tgcgcggacg tgccgcaggc cttgagcagc acccagggct gcgcgatcgt ggcgccgagc
     1741 tcgcgcaggg tgggcgacag gtgcgggaag acgaagcgcg ccgcctgctg cggcaggccg
     1801 acctcgatgc gaaatccgtc cgcgtgggcc acgggcggcg gcgtgtcgcg gctgcgagcc
     1861 cagcccgcga tccaggcgag gaagatctcg tcgtgcccga aggcgaagga atcgtcgatc
     1921 gacatggggc gccattatcg cggccccgat gcgcccgtca gaggatcatc ggcgtgcagc
     1981 gatgcaggtc gactggcggt tcgcacagga ccgcatggaa acgttgcagc acggccggcg
     2041 ccatgcgcga cgcctcgggc gacaggtaga acgcgtagtc gcccacgcgg ccgtgcgcca
     2101 tgaacacctc cgccgacggc ggctcgcccg cgctgccata ggcgtcctcc agggccagca
     2161 cgagctccgc ggcctccgat tcatgcgcgc cgcggcggtc gaacttgatg caatgccaat
     2221 ccatggccgg ctcctcgaag gcggtcgtct gctcgacagg gcaaccggcg tgcccgcccg
     2281 cgcccgcggc tgctgccaca atgcgccggc gccccggtgg tgaaattggt cagacactcg
     2341 ggacttaaaa tcccgcgcct tcacgggcat gccggttcga gtccggcccg gggcaccacg
     2401 ccgaacgcat caggacgtgt ccgcgccacc gggcgccggg gcatgcgatg cccgcgcgca
     2461 agcgccggct gatgcctctc gcaccactcg tgcgcgatgc ctcccgggcc atcgcccggc
     2521 ggtgtcgcgc ccggactcgc aggccgaacc tttacaaaca tgttcgcgcc cgcgccgcgc
     2581 gcgcgagcct gtagaaatgc gaccttgcgc gcgttgggcg cctggcacga gagggttgca
     2641 acgatgattc gacaaggcgc gatggcgcga ctggccgcgc tcatgagctg cgtggcgctg
     2701 agcgcgtgcg gcggcggcga tggcgccgag gcccaggccg caccgagcgc ggctcccacg
     2761 cccgctcccg tacccgcgcc cgcgccggcc cccacgccgg caccgactcc ggcgcccaca
     2821 ccgacaccaa cgccgacgcc ggctcctgca ccgacgcccg cgccgacccc tgcgcccacg
     2881 ccggccccag cgccgacgc
//
LOCUS       DATCRO010000295         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_745968, whole genome shotgun sequence.
ACCESSION   DATCRO010000295 DATCRO010000000
VERSION     DATCRO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745968"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VIN75_07995"
     CDS             <1..476
                     /locus_tag="VIN75_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8974144.1"
                     /translation="VGPRCHCGNVGCVETMAGGPAIAREATEAAHAGQSAMLAERLAA
                     NGVLTPEDVGNASRAGDAAANQIVQRAGTQIGQMLASVVNFFNPSHVYVGGGVTRIGP
                     LFLASLRQCVYQRSLALSTRHLEVGYMPLGDQAGLIGAGVLALQETLRGRAKVGA"
     gene            535..2034
                     /locus_tag="VIN75_08000"
     CDS             535..2034
                     /locus_tag="VIN75_08000"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY8974145.1"
                     /translation="MTGVPRSVTVAFDGIVKSFGAVQVLHGVSFELAPGRVVGLLGEN
                     GAGKSTLMKILAGYEAPGGGELRIDGAAASFDGPRAAEARGIVMIHQEFALAEDLTIA
                     QNVFLGHERRRGWWLDDKAMDAETARVLKLVGLQASPGTPVRQLIVAEKQLVEIAKAL
                     QRDARVLVMDEPTATLTPGETERLFSLIAQLKADGVTVVYISHKLDEIERITDEVVVM
                     RDGRFVARSATRELTRHQMANLMVGREIADLYPPRDARPAAAPLLAVRGLNVPGWARE
                     LDFDVAPGEILGFAGLVGAGRTEAFEGLLGLRPHTVRSLRIDGRAVRIRTPREAASHG
                     LTYLSEDRKGKGLHVHFGLAENLTLMALQRYATPWLHPGAERDALQAAVGEFGIRAGA
                     LDIPASSLSGGNQQKLALAKVLHPGPRVLVLDEPTRGVDVGAKRDIYFLIQRLAREGK
                     AIVVISSELVELIGICHRIVVMRAGAVQATLAADELTEEALIAHATGLH"
     gene            2108..3049
                     /locus_tag="VIN75_08005"
     CDS             2108..3049
                     /locus_tag="VIN75_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974146.1"
                     /translation="MHGLGPVLGLVVLCIAGFLLNHDFATIDNAMNVLTRTAFIGIIS
                     VGMCFVIISGGIDLSVGSMAALIAGGVILLMNALGARHLAPWLTVVLGAGFAVMLGAL
                     FGLAHGLLITRGRIEPFIVTLGTLGIFRATLTWLSDGGAITLDNDLADAYAPVYYAAV
                     LGIPVPVWVFLVVAVLGAAILNRTAYGRYVQAIGSNEQVARYAAVDVDFTKLVTYMLL
                     GACVGVATLLYVPRLGSASPTTGLLWELEAIAAVIVGGTALKGGAGSITGTVVGAILL
                     SVISNILNLTSIISVYLNAAVQGGVIIVVAFMQRRRR"
     gene            3105..>3823
                     /locus_tag="VIN75_08010"
     CDS             3105..>3823
                     /locus_tag="VIN75_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8974147.1"
                     /translation="MKPIQRRLVLALGAASLALAAPLAFAQNKVTLGVAIPTADHGFT
                     GGIVWWANQAKKELEKKYPDLKVIVKTSKDPGEQANQLQDLVTANKINALVIFPHESA
                     ALTKPVQQVHSKGVYVTVVDRGLTDESGQDAYVAGDNTAFGKVPAEYIAKALNGKGDI
                     VALRGIATTLDNERMDAFNGVLKNYPDIHLLDAKYANWNRDDAFKTMQDFLTRFKHID
                     AVWAADDDMAVGVLKAIDQAKR"
BASE COUNT          569 a         1327 c         1378 g          549 t
ORIGIN      
        1 cggtggggcc gcgctgtcat tgcggcaacg tcggctgcgt cgagacgatg gccggcggcc
       61 cggcgatcgc gcgcgaggcc accgaagcgg cgcacgccgg ccagagcgcg atgctcgccg
      121 agcgtctggc cgccaacggc gtgctcacgc ccgaggacgt gggcaacgcc agccgcgccg
      181 gcgacgccgc cgccaaccag atcgtgcagc gcgcgggcac gcagatcggc cagatgctcg
      241 cgtcggtcgt caacttcttc aacccgtcgc acgtgtacgt cggcggcggg gtcacgcgca
      301 tcgggccgct gttcctggcc tcgctgcgcc agtgcgtcta ccagcgctcg ctggcgttgt
      361 cgacgcgcca tctcgaagtc ggctacatgc cgctgggcga ccaggccggc ctgatcggcg
      421 cgggcgtgct cgcgttgcag gagacgctgc gcggccgggc gaaggtgggc gcatgaaccc
      481 gcacggccac ccgaaaggcg aatcgaccgc agtccgcggg acggaggttt caccatgacg
      541 ggcgtgcccc gctcggtcac cgtggccttc gacggcatcg tgaagtcctt cggcgcggtg
      601 caggtgctgc acggcgtgag cttcgagctg gcgccggggc gcgtcgtcgg gctgctgggc
      661 gagaacggcg ccggcaagag cacgctgatg aagatcctgg ccggctacga agcccccggc
      721 ggcggcgagc tgcggatcga cggcgccgcc gcgagcttcg acggcccgcg cgcggccgag
      781 gcgcgcggca tcgtgatgat ccaccaggag ttcgcgctgg ccgaggacct gaccatcgcg
      841 cagaacgtgt tcctcggcca cgagcgacgc cgcggctggt ggctggacga caaggcgatg
      901 gacgcggaga ccgctcgcgt gctcaagctc gtcggcctgc aggcgagtcc cggcacgccg
      961 gtgcgccagc tgatcgtcgc cgagaagcag ctggtcgaga tcgccaaggc gctgcagcgc
     1021 gacgcgcgcg tgctggtgat ggacgagccc accgccacgc tcacgccggg cgagacggag
     1081 cgcctgttct cgctgatcgc gcagttgaag gccgacggcg tgacggtggt ctacatctct
     1141 cacaagctcg acgagatcga gcgcatcacc gacgaggtcg tcgtgatgcg cgacggccgc
     1201 ttcgtcgcgc gctcggccac gcgcgagctc acgcgccacc agatggccaa cctcatggtg
     1261 ggccgcgaga tcgccgacct gtatccgccc agggacgcgc ggcccgccgc cgcgccgctg
     1321 ctggccgtgc gcgggctgaa cgtgccgggc tgggcgcgcg agctcgactt cgacgtcgcg
     1381 ccgggcgaga tcctgggctt cgccgggctg gtcggggcag ggcgcaccga agccttcgag
     1441 ggcctgctgg gactgcgtcc gcacacggtg cgatccctcc ggatcgatgg ccgcgcggtg
     1501 cgcatccgca ccccgcgcga ggccgcgtcg cacgggctca cgtatctcag cgaggaccgc
     1561 aagggcaagg gcctgcacgt gcacttcggg ctggccgaga acctgacgct gatggcgctg
     1621 cagcgctacg cgacgccgtg gctgcacccg ggcgcggagc gcgacgcgct gcaggcggcg
     1681 gtcggcgagt tcggcatccg cgccggcgcg ctcgacatcc cggcgtcgtc gctgtcgggc
     1741 ggcaaccagc agaagctcgc gctggccaag gtgctgcatc cgggcccgcg cgtgctggtg
     1801 ctcgacgagc ccacgcgcgg cgtggacgtc ggcgccaagc gcgacatcta tttcctgatc
     1861 cagcggctcg cgcgcgaggg caaggcgatc gtcgtcatct cgtccgagct ggtcgagctg
     1921 atcggcatct gccaccgcat cgtcgtgatg cgcgccggcg ccgtgcaggc cacgctggcc
     1981 gcggacgaac tcaccgagga ggccctgatt gcccacgcca ccggactcca ctgaagtgcc
     2041 cgcctccgcg cgcggggacg cgggcgcgct cgcgggcgtc acgcgcgcgc gccgcgcctg
     2101 ggcgcgcctg cacgggctcg ggcccgtgct ggggctggtc gtgctgtgca tcgccggatt
     2161 cctgctgaac cacgacttcg ccacgatcga caacgcgatg aacgtgctca cgcgcacggc
     2221 gttcatcggc atcatctcgg tgggcatgtg cttcgtgatc atcagcggcg gcatcgacct
     2281 gtcggtgggt tcgatggcgg cgctgatcgc gggcggcgtc atcctgctga tgaacgcgct
     2341 gggcgcgcgc cacctcgcgc cgtggctgac cgtggtgctg ggcgccggct tcgcggtgat
     2401 gctgggcgct ctgttcgggc tcgcgcacgg gctgctgatc acgcgcgggc gcatcgagcc
     2461 cttcatcgtg acgctgggca cgctgggcat cttccgcgcg acgctgacgt ggctgtcgga
     2521 cggcggcgcc atcacgctgg acaacgacct ggccgacgcc tacgcgccgg tctactacgc
     2581 ggcggtgctg ggcatcccgg tgccggtgtg ggtgttcctg gtcgtcgcgg tgctgggcgc
     2641 cgcgatcctg aaccgcacgg catacggccg ctacgtgcag gccatcggct ccaacgagca
     2701 ggtggcgcgc tacgccgcgg tcgacgtcga cttcaccaag ctcgtgacct acatgctgct
     2761 gggcgcctgc gtgggcgtgg ccacgctgct gtacgtgccg cggctgggct cggcatcgcc
     2821 gaccaccggc ctgctgtggg agctggaggc gatcgccgcg gtgatcgtcg gcggcacggc
     2881 gctcaagggc ggggcgggct cgatcaccgg cacggtggtg ggcgcgatcc tgctgtccgt
     2941 catcagcaac atcctgaacc tcaccagcat catcagcgtc tacctgaacg cggcggtgca
     3001 gggcggcgtg atcatcgtcg tggccttcat gcagcggcgg cggcggtgat cccttgcggc
     3061 ccgcaggccg cgtttccttc gatcgacaac aggcaggaga catgatgaaa ccgatccaac
     3121 gcagactcgt cctcgcgctc ggcgccgcat cgctggcgct ggccgcgccg ctcgccttcg
     3181 cgcagaacaa ggtcacgctc ggcgtggcga tccccacggc cgaccacggc ttcaccggcg
     3241 gcatcgtctg gtgggcgaac caggccaaga aggagctcga gaagaagtac cccgacctca
     3301 aggtcatcgt gaagacgtcc aaggacccgg gcgagcaggc gaaccagctg caggacctcg
     3361 tgacggccaa caagatcaac gcgctggtga tcttcccgca cgagtcggcg gcgctgacca
     3421 agcccgtgca gcaggtgcat tccaagggcg tgtacgtgac ggtggtcgac cgcggcctga
     3481 ccgacgagag cggccaggac gcctacgtgg ccggcgacaa caccgcgttc ggcaaggtgc
     3541 ccgccgaata catcgccaag gcgttgaacg gcaagggcga catcgtggcc ctgcgcggca
     3601 tcgccaccac gctggacaac gagcgcatgg acgcgttcaa cggcgtgctg aagaactacc
     3661 ccgacatcca cctgctggac gccaagtacg ccaactggaa ccgcgacgac gcgttcaaga
     3721 cgatgcagga cttcctcacg cgcttcaagc acatcgatgc cgtgtgggcg gccgacgacg
     3781 acatggccgt cggcgtgctc aaggcgatcg accaggccaa gcg
//
LOCUS       DATCRO010000296         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_993083, whole genome shotgun sequence.
ACCESSION   DATCRO010000296 DATCRO010000000
VERSION     DATCRO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993083"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIN75_08015"
     CDS             <1..195
                     /locus_tag="VIN75_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974148.1"
                     /translation="AYLSGPRHFPPEMRLSQRVLQRIAAASVPLEKGPAGQGYRPQNI
                     VNSYVMLDWSGAAPHVVVEP"
     gene            521..2389
                     /locus_tag="VIN75_08020"
     CDS             521..2389
                     /locus_tag="VIN75_08020"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY8974149.1"
                     /translation="MNRPAMEGLRLNVPAYVKHDRLVSWVAEIAALTQAAEVYWCDGS
                     QAEYDRLCDLLVSAGTFRRLNPAKRANSYLACSDPSDVARVEDRTFICSEKKESAGPT
                     NNWESPEKMRALLQHGQAGTPALFAGSMRGRTMYVVPFSMGPLGSPISHIGVELSDSP
                     YVAVNMRLMTRMGRGALDVLGADGDFVPCVHTVGAPLEPGQKDVSWPCNKTKYIVHYP
                     ETREIWSYGSGYGGNALLGKKCFALRIASNMGRDEGWLAEHMLILGVTSPQGVKRHIA
                     AAFPSACGKTNFAMLIPPAGMPGWKVTTIGDDIAWIKPSADGRLMAINPEAGYFGVAP
                     GTNQLTNPNCMASLTRDVIFTNVALTDDGDVWWEGMGEAPAHCIDWQGKDWTPAIAKE
                     TGAKAAHPNARFTVAATNNPALDPAWDDPKGVAIDAFIFGGRRSTTVPLVTEARDWIE
                     GVYMAATMGSETTAAAVGQQGVVRRDPFAMLPFTGYNMADYFQHWLDVGARLESLGAK
                     RPHIFCVNWFRKGPDGKFVWPGYGENMRVMEWMLGRIDGTAQGQENVFGTSPRYEDIN
                     WTGLDFTRAQFDSVIGIDKAAWQQELTMHDELFTKLAYHLPQELQATKGRIAAELG"
     gene            2481..3257
                     /locus_tag="VIN75_08025"
     CDS             2481..3257
                     /locus_tag="VIN75_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8974150.1"
                     /translation="MRLQILSDLHLETETFEPEPAPGAELLVLAGDIDREWEGLRRYA
                     DWPVPVLFVPGNHEFDNRDVDQAAIELRAFCDELGLRMLHRDTAILVDDRGRRIRFCG
                     TVRWSDFDLFGATGREKALRSGSYFQRVMNATRHGQPFDAEAVRVLGLSCRDWLERTL
                     PEPALGRWDATVAITHFAPSLRSADPRYGRQPTTASFCNADDDLIPRVDLWIHGHLHC
                     RHDYLVERPGQHASRVVCQARGLEKKGEAEGFDPMRLIEV"
     gene            complement(3297..3527)
                     /locus_tag="VIN75_08030"
     CDS             complement(3297..3527)
                     /locus_tag="VIN75_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974151.1"
                     /translation="MTNASRPTFLRNFGRGATPPRRPPDLADMGTAFALDEALESGHI
                     DSIVPGDPSPPPVRAAVPVAPWRLWLGRKLGA"
     gene            3745..3969
                     /locus_tag="VIN75_08035"
     CDS             3745..3969
                     /locus_tag="VIN75_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974152.1"
                     /translation="MRATQLQSLTWGTVTAAIGCAVWLSSSDQAALFCSVGYRATSQA
                     MGLDLPAEPTQAGKAAQAPGSSAARQAQAG"
     gene            4204..>4523
                     /locus_tag="VIN75_08040"
     CDS             4204..>4523
                     /locus_tag="VIN75_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974153.1"
                     /translation="MKVIVLGAGIIGVATAWHLLEEGHEVTVVDRQPEAALETSFANG
                     AQISVCFCEPWANAGAPFKVARWMLQADSPLLFRPRLDPHQWRWALSFLTQCTDAAFA
                     RNVE"
BASE COUNT          708 a         1606 c         1533 g          676 t
ORIGIN      
        1 gcgtacctgt ccggcccgcg ccacttcccg cccgagatgc gcctgagcca gcgcgtgctg
       61 cagcgcatcg ccgccgccag cgtgccgctg gagaagggcc cggcgggcca gggctaccgg
      121 ccgcagaaca tcgtcaacag ctacgtgatg ctggattggt ccggcgcggc gccgcacgtg
      181 gtcgtcgagc cttgagccga cgcgccggaa cacgccttgc cgtgtggcgg ccaggctgcg
      241 ggccggttcg tgcatcgatc tattccggaa atgcatgatc cgcgtccgtc gcctggttac
      301 gacggaatta cactgtggac tcgatgcttt cccggtactt ttcagggata acccgcgatt
      361 tccgcgagtt cctcggtgta actcccgttc gccccctcgg ggcggccggg tacggtgcga
      421 acaggcatcg cgagattggg cgagccccgc gccacggcag cggccgtggc agcgaggttc
      481 gcccaatctt gtcttcgaac cttgtcattg gagtctcctc atgaaccgtc ccgccatgga
      541 agggctgcgc ctgaacgtgc ccgcctacgt caaacatgac cgcctggttt cctgggtcgc
      601 cgagatcgcg gcgctcacgc aggccgccga ggtctactgg tgcgacggct cgcaggccga
      661 gtacgaccgc ctgtgcgacc tgctggtgtc ggccggcacc ttcaggcgcc tgaacccggc
      721 caagcgcgcc aacagctacc tcgcctgctc cgacccgtcg gacgtggcgc gcgtggagga
      781 ccgcaccttc atctgcagcg agaagaagga atcggccggc ccgaccaaca actgggaatc
      841 gcccgagaag atgcgcgcgc tgctgcagca cggccaggcc ggcacgccgg cgctgttcgc
      901 cggctcgatg cgcggccgca cgatgtacgt ggtgccgttc tcgatgggcc cgctgggctc
      961 gccgatctcg cacatcggcg tcgagctgtc cgacagcccg tacgtggccg tcaacatgcg
     1021 cctgatgacc cgcatgggcc gcggcgcact cgacgtgctg ggcgccgacg gcgacttcgt
     1081 cccctgtgtg cacaccgtgg gcgcgccgct ggagccgggc cagaaggacg tctcctggcc
     1141 ctgcaacaag accaagtaca tcgtccacta ccccgagacg cgcgagatct ggagctacgg
     1201 ctcgggctac ggcggcaatg cgctgctggg caagaagtgc ttcgcgctgc gcatcgcgtc
     1261 caacatgggc cgcgacgagg gctggctggc cgagcacatg ctgatcctgg gcgtgaccag
     1321 cccgcagggc gtcaagcgcc acatcgcggc ggcgttcccg agcgcctgcg gcaagaccaa
     1381 cttcgccatg ctgatcccgc cggcgggcat gcccggctgg aaggtcacga ccatcggcga
     1441 cgacatcgcc tggatcaagc cgagcgccga cggccgcctg atggccatca accccgaggc
     1501 cggctacttc ggcgtcgccc cgggcaccaa ccagctgacc aacccgaact gcatggccag
     1561 cctcaccagg gacgtcatct tcaccaacgt cgccctaacc gacgacggcg acgtgtggtg
     1621 ggaaggcatg ggcgaggcgc ccgcgcactg catcgactgg cagggcaagg actggacgcc
     1681 cgccatcgcc aaggagaccg gcgccaaggc cgcgcacccg aacgcccgct tcacggtggc
     1741 cgccaccaac aacccggcgc tcgacccggc ctgggacgac cccaagggcg tggcgatcga
     1801 cgcgttcatc ttcggcggcc gccgctccac cacggtgccg ctggtgaccg aggcgcgcga
     1861 ctggatcgag ggcgtctaca tggccgcgac catgggctcc gagacgaccg ccgcggccgt
     1921 cggccagcag ggcgtggtgc gccgcgaccc gttcgcgatg ctgccgttca ccggctacaa
     1981 catggccgac tacttccagc actggctcga cgtcggcgcg cggctggagt cgctgggcgc
     2041 caagcgcccg cacatcttct gcgtgaactg gttccgcaag ggcccggacg gcaagttcgt
     2101 gtggccgggc tacggcgaga acatgcgcgt catggagtgg atgctgggcc gcatcgacgg
     2161 cacggcgcag gggcaggaga acgtcttcgg caccagcccg cgctacgagg acatcaactg
     2221 gaccggcctg gacttcaccc gcgcccagtt cgacagcgtg atcggcatcg acaaggccgc
     2281 ctggcagcag gagctgacga tgcacgacga gctgttcacg aagctcgcgt accacctgcc
     2341 gcaggaattg caggccacca agggccgcat cgcggcggag ctgggctgac agatttcaag
     2401 tcttcctcga tggccgcctt cgggcggcct ttttttcggg tcgcaggacg ccgcggtcgg
     2461 agcggtatgc tgccgccacg atgcgactgc agatcctgtc cgacctccac ctcgagaccg
     2521 agaccttcga gcccgagccc gcgcccggcg ccgagctgct ggtcctggcg ggcgacatcg
     2581 accgcgagtg ggaaggcctg cgccgctacg ccgactggcc cgtgccggtg ctgttcgtgc
     2641 ccggcaacca cgagttcgac aaccgcgacg tcgaccaggc cgcgatcgag ctgcgcgcgt
     2701 tctgcgacga gctgggactg cgcatgctcc atcgcgacac ggccatcctc gtcgacgacc
     2761 gcggccgccg catccgcttc tgcggcaccg tgcgctggag cgacttcgac ctgttcggcg
     2821 ccaccggccg cgagaaggcg ctgcgctcgg gctcgtactt ccagcgcgtg atgaacgcga
     2881 cccgccacgg ccagccgttc gacgccgagg ccgtgcgcgt tctggggctg tcatgccgcg
     2941 actggctgga gcgcacgctg ccggagccgg cgctcgggcg ctgggacgcc acggtggcga
     3001 tcacgcattt cgcgccgagc ctgcgcagcg cggatccgcg ctatggacga cagccgacga
     3061 cggccagctt ctgcaacgcg gacgacgacc tgatcccgcg cgtggacctg tggatccacg
     3121 gccacctgca ctgccgtcac gactatctgg tggaacggcc tggccagcac gcctcccgcg
     3181 tggtgtgcca ggcgcgcggc ctggagaaga agggcgaggc cgagggcttc gacccgatgc
     3241 ggctgatcga ggtgtgagac gcggcgcgcg ccgcgcgcac gtcggtcagg agaccatcac
     3301 gcgccgagct tgcggcccag ccacaggcgc cacggcgcca ccggcacggc ggcgcgcacg
     3361 ggcggcgggc tcgggtcgcc cggcacgatc gagtcgatgt ggccgctctc cagcgcctcg
     3421 tcgagggcga aggcggtgcc catgtcggcg aggtccggcg gccggcgtgg cggcgtcgcg
     3481 ccgcggccga aattgcgcag gaacgtgggg cgggaggcgt tggtcatcgg tctctccagg
     3541 tttccaaccg tgagaccagt gtgtgggaat gtttccgagt cggtgcgcca aatagtgtgt
     3601 cgtatgcccg acagttcgtg tgtatcgaat gcacgactcc cccgaacgga cgatggcagg
     3661 gtcatggctt cggacttaag ctgcaagcgt cgatgccgag ggtgcagctg cgcacccgcg
     3721 caaaccacct tgggagccgc cgccatgcgc gccacgcaac ttcaatctct cacctggggc
     3781 accgtcaccg ctgccatcgg ctgtgcggtg tggttgtcgt cttcggacca ggcggcgctg
     3841 ttctgctccg tcggctatcg cgccaccagc caggcgatgg gcctcgacct gccggccgaa
     3901 cccacgcagg ccggcaaggc ggcgcaggcg ccgggttcga gcgcggcccg ccaggcgcag
     3961 gccggctgac acggcccgct cggcgcctgc ccggcgccct cttgggtcgc gaacgagccg
     4021 ctgcagcgga cgtccatgcg cgcccgcgcc aaccacccac ccacgatcag tagatcgcct
     4081 actgaacttg gaattcgatt aatccagctt cagttacatc gccacctctc cgagaatccg
     4141 gtcgcagccc gacggtgccg ctggtcgttc ccggccggcc gccgagcggc gaagggattc
     4201 gcgatgaaag tgatcgttct gggcgccggc atcatcggcg tcgccaccgc ctggcacctg
     4261 ctggaagaag gccacgaggt gaccgtcgtc gaccgccagc cggaagccgc gctggagacg
     4321 agctttgcca acggcgccca gatctcggtg tgcttctgcg agccctgggc caacgccggc
     4381 gcgccgttca aggtcgccag gtggatgctg caggccgatt cgccgctgct gttccgcccg
     4441 cgcctcgatc cgcaccagtg gcgctgggcc ctgagcttcc tcacgcagtg caccgacgcc
     4501 gccttcgcgc gcaacgtcga aca
//
LOCUS       DATCRO010000297         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_869242, whole genome shotgun sequence.
ACCESSION   DATCRO010000297 DATCRO010000000
VERSION     DATCRO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869242"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1348)
                     /locus_tag="VIN75_08045"
     CDS             complement(173..1348)
                     /locus_tag="VIN75_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974154.1"
                     /translation="MTDKPAPSAAADRPDLGWAALLRLSCAYAIATNGTLLMPLFVGV
                     LMRRFAIGDDAATGVAALEIGGIAISCAVFPRWIARVPRRLAWIATLGTLLAQAASAW
                     MPSLAAVGAARLVTGSFEGVLFVVVAASLSNRPAAERAWGVIVLASGVFDCALLVGAY
                     ALPQDAVLQWVFVIVAIAFALIAWPAATAGADAVPPTSSALPPPRAIRWRVLLPIWAV
                     MALVYSVLSAQWALADVVGHQIGLPPERIGPLLALVSLLGTVGALAASHRRSHALRVP
                     ILWIAQAVMAGGVLWFFVVRGAAGFFAAQLLVSAAFYAVTPFLTSRLSGLDADGSLLS
                     RSIVITFVAAFAGTALAGTALTQLGGLGCGIALGLCALVAMPFTWTAFAGELRAKAG"
     gene            1411..2079
                     /locus_tag="VIN75_08050"
     CDS             1411..2079
                     /locus_tag="VIN75_08050"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8974155.1"
                     /translation="MTNPTPDTAPAPGALPGRRPRQERSRLRVENILAIALRLMVEQG
                     AEALAMREIARRAEVQISSIYQYFPSKSAIIRELAQRDLARVRQLLQEAVDALLAGPR
                     TPPISAAVDHLVDAYFAHYRDQPEALAVWAGAQSDPSLRELDQEDTRSTAEFLVAPLL
                     HLLGRPQWDGVHALSLLISEVTGAAARLALSLESPLREQLIAQHKAMLVATLESHKAR
                     GKNA"
     gene            2138..>2970
                     /locus_tag="VIN75_08055"
     CDS             2138..>2970
                     /locus_tag="VIN75_08055"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8974156.1"
                     /translation="MSADYDTYRSALVGRRLPAAFVDLDALDANLADLRRRAGDLPIR
                     LVTKSVRCVSILRRALASSPKFQGLMCYSPAEAAWLASLGFHDLLVAYPSVEPDDLRA
                     VAAQVRAGRSITLMVDAAAQVQRIAQVAQAEGVVQPLAIDLDMSSDFPGLHFGVFRSP
                     VDGVAAALALATEIARHPSLRLDGLMGYESQIAGLMDDVPGQALKSAAIRLLKRRSIP
                     EINERRRATVAALEAAGHALRFVNGGGTGSFESTRLDASVTELAAGSGLYVPTLFDHY
                     RA"
BASE COUNT          456 a         1091 c         1060 g          363 t
ORIGIN      
        1 ggactcgggg cgcaacgcac gacagactcg caagcgtgca agcgcgtcct gcaagggctt
       61 gtccagaggg ccgggacggt cggcgcgcag gggcgaacgc ctggcattcc gaatggccgc
      121 cagcgggtgt cgagatcgag cgtctgattt tcgccttcgg gctcgctggt cgtcagccgg
      181 cctttgcccg aagctcgcct gcgaacgccg tccacgtgaa cggcatcgcc accagcgcgc
      241 agagcccgag cgcgatgccg cacccgagcc cgcccagttg cgtcagcgcc gtgcccgcca
      301 gcgccgtgcc cgcgaacgcg gccacgaagg tgatgacgat gctgcgtgac aggagcgagc
      361 cgtcggcgtc caggccggac aggcgcgacg tgaggaacgg cgtgacggcg tagaacgcgg
      421 ccgacaccag cagctgcgcg gcgaagaacc cggccgcgcc gcgcacgacg aagaaccaca
      481 gcacgccgcc ggccatcacg gcttgcgcga tccacaggat gggcacgcgc agcgcatggc
      541 tgcgccggtg cgaggccgcg agcgcgccga cggtgcccag cagcgacacc agcgccagca
      601 gcggcccgat gcgctcgggc ggcaggccga tctggtggcc cacgacatcg gccagcgccc
      661 attgcgccga cagcacgctg tagacgagcg ccatcaccgc ccagatcggc agcagcacgc
      721 gccagcggat cgcgcgcggc ggcggcagcg ccgacgacgt cggcggcacg gcgtcggccc
      781 ccgccgtggc cgcgggccag gcgatgagcg cgaacgcgat cgcgacgatc acgaacaccc
      841 actgcagcac ggcgtcctgc ggcagcgcgt acgcgcccac cagcagcgcg cagtcgaaca
      901 cccccgacgc cagcacgatg acgccccacg cgcgctcggc cgcgggccgg ttcgacaggc
      961 tggccgcgac cacgacgaac agcacgcctt cgaacgatcc ggtgaccagc cgcgcggcgc
     1021 ccacggccgc caggctgggc atccacgcgc tggcggcttg cgcgagcagc gtgccgagcg
     1081 tcgcgatcca cgccaggcgc cgcggcacgc gcgcgatcca gcgcgggaac acggcgcagc
     1141 tgatggcgat gccgccgatc tcgagcgcgg ccacaccggt ggcggcgtcg tcgccgatgg
     1201 caaagcgtcg catcagcacg cccacgaaca acggcatcag cagggtgccg ttggtggcga
     1261 tcgcataggc gcaggacagt cgcagcagcg cggcccatcc caggtcggga cggtcggcgg
     1321 cggccgaagg tgcgggtttg tccgtcatgg cgccggcttg aaagtgatca atgttcacaa
     1381 tatgatgtga tcattgatca catgtcaatg atgaccaacc cgacccctga taccgccccg
     1441 gcgcccggcg ccctccccgg ccggcggccg cgccaggagc gcagccgcct gcgggtggag
     1501 aacatcctgg ccatcgcgct gcggctgatg gtggagcagg gcgcggaggc gctggcgatg
     1561 cgcgagatcg cccgccgcgc cgaggtgcag atcagctcga tctaccagta cttcccgtcg
     1621 aagtcggcca tcatccgcga gctcgcgcaa cgcgacctcg cgcgcgtgcg ccagctgctg
     1681 caggaggccg tcgacgcgct gctcgcgggc ccgaggacgc cgcccatctc ggcggccgtc
     1741 gaccacctcg tcgacgccta cttcgcgcac taccgcgacc agcccgaggc gctggccgtg
     1801 tgggccggcg cgcagagcga cccgagcctg cgcgagctcg accaggagga cacgcgcagc
     1861 accgccgaat tcctcgtggc gccgctcctg cacctgctgg gccggccgca gtgggacggc
     1921 gtgcacgcgc tgtcgctgct catctcggaa gtgacgggcg cggccgcgcg gctcgcgctg
     1981 tcgctcgaat cgccgctgcg cgagcagttg atcgcgcagc acaaggcgat gctcgtggcg
     2041 acgctcgagt cgcacaaggc gcgcggaaag aacgcatgag cgccgcccgg ccgcccgaag
     2101 gcgctcgccc cgcagtccgc aggacggagg ttgccttatg agcgccgact acgacacata
     2161 ccgaagcgcg ctcgtcgggc gccgactgcc cgcggcgttc gtcgacctcg acgcgctgga
     2221 tgccaatctc gccgacctgc ggcggcgcgc cggcgacctg ccgatccggc tggtgacgaa
     2281 gtcggtgcgc tgcgtctcga tcctgcggcg cgcgctcgcg tcgtcgccga agttccaggg
     2341 cctgatgtgc tactcgccgg ccgaggccgc gtggctcgcg tcgctgggct ttcacgacct
     2401 gctcgtggcc tacccgtcgg tcgagccgga cgacctgcgc gcggtcgcgg cgcaggttcg
     2461 cgcggggcgc tcgatcacgc tgatggtcga cgccgcggcg caggtgcagc gcatcgcgca
     2521 ggtcgcgcag gccgagggcg tcgtgcagcc gctggcgatc gacctggaca tgtcgtccga
     2581 cttccccggc ctgcacttcg gcgtgttccg ctcgcccgtc gacggcgtcg ccgccgcact
     2641 ggcgctggcc acggagatcg cgcgccatcc gtcgctgcgg ctcgacggcc tgatgggcta
     2701 cgaaagccag atcgccgggc tgatggacga cgtgccaggg caggcgctca agagcgcggc
     2761 gatccggctg ttgaagcgcc gctcgattcc cgagatcaac gagcgccgcc gcgcgacggt
     2821 cgcagcgctc gaggcggcgg gccacgcgct gcgcttcgtc aacggcggcg gtacgggcag
     2881 cttcgagagc acgcgcctgg atgcgagcgt caccgagctg gcagccggct ccggcctgta
     2941 cgtgcccacg ctgttcgacc actaccgcgc
//
LOCUS       DATCRO010000298         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_171874, whole genome shotgun sequence.
ACCESSION   DATCRO010000298 DATCRO010000000
VERSION     DATCRO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171874"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2300)
                     /locus_tag="VIN75_08060"
     CDS             complement(<1..2300)
                     /locus_tag="VIN75_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974157.1"
                     /translation="MDSNLFKSMMLRAAAAIALSAAMAGSAVAAGTVTLQASPSTTTL
                     PDGRTVPMWGYTCGDVLPASAPSTGATCTAANGAAQNGITWQPPLITVPAGQLTINLA
                     NQLPAGLPTSVIIDGQVGGGLGGSRTTAPSPTHGLQGTTWPGTLGGIDPATSAITLGA
                     SGAGYNAPPVVTITNANAHGGAGATAVAYVGNGAVTRVVVSNPGSGYSAADSLTVSIA
                     PPPCTPSATCVPATATIDIGVLSSSDGSPTFVPPPQVDRVRSLASEVANGTTGSLVWA
                     NLRPGTYVLHSGTETSIQHTMGLYAILVVTDPAGATTAYGNAIDKDVPLLFSEIDPEF
                     NDGVTKAVGTAGFDPRAVWNGQPGMCGDPTVHSCYPPIVNYSPLYYLINGTAFDRTNM
                     AASMFAGPTGTPTAGTVLLRLANAGLRMHVPSVVGAPMTLLAEDGNALHGPTRIQDTV
                     LLTAGKTYDVTIAPAVASGAYVASAYPVFDRQLSLSTGNARDGGMQAYISVNGGAASG
                     VGSAALNTSASANDKTYYCSAGQTLAVSNPGLGLLAGATGVNGVALGAMTGDLSATTL
                     TLLSNGTFTYVPSSAASCGGTFQYLPNGNLALAKTVTITECDATSSVAGCGFDTGLKA
                     NPVQFVSNVASGYVSTPPGVLSGVTNPAGLALTASGAGVNADGSFSVAGPGAAGNCSG
                     WDATKGALPAGATCALVPFTVTNVQGSTSSSSAIVAFQAGSGLTINVVDAVDPTKAIT
                     DYRWIIEEDRTFWIDPKCQVNSTNPAL"
     gene            complement(2318..3958)
                     /locus_tag="VIN75_08065"
     CDS             complement(2318..3958)
                     /locus_tag="VIN75_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY8974158.1"
                     /translation="MQLRRVAAVAAWATLASGAHAASPGITGPAFDLVAKDGYISQPD
                     GASVYTWGYGCNSAPASFVPATIANAACPAGQMQLPGPTLVVNQGDTVSVTLHNGLPA
                     AAGSTSILFPGFHVTSTGGSPGVLAQEAVPGGAVTYTFVADTPGTHSYYSGTQGDLQI
                     EMGMFGALIVLPAHVTSNTYAPLNGAVPSSYTPQAAGGGLPNTCFAASPASAGATLPN
                     GAPDYRLAAAAYDHPEACYDREYLIQLSEMDIRIHNEAQAQKDLACANPAGCMVIDTE
                     PYHPAYFMVNGRSMPDDMDPNYASQYPLQPYNGNPHMHPGELVLLRIIGQGRMQHPLH
                     EHANHVRVLARDGNMLLSPKGNLAGPLLFTTSTTPGQTIDGIFQYTGKGLNWDVYGHT
                     ANAALDGSPEGNGGAPCTPDKNGYYTSGSNPPAPFTAPNYFEWCEDHMKPLEAHPFGS
                     VAGGGPVTLPDPNILTNGAWYSGSPYLGPDANARSVGNTPIPPFGTIANSPSTEAGFA
                     FMWHSHNEREITTNNIFPGGMMMMMLVDPWVFQIIEAN"
     gene            complement(3993..>5590)
                     /locus_tag="VIN75_08070"
     CDS             complement(3993..>5590)
                     /locus_tag="VIN75_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8974159.1"
                     /translation="YMFSFGPLSGLGNIVHGLPGTVSASEFNQSNLDAQGNVLDVVKI
                     GAPDSADYAFNGAIGLAPDINAEQVAGVTFNIDPATDLPITGTRYKVGDAVVFTGGGG
                     SGAVGAVATVSGTGAILTISITNPGVNYTSVPDVSIVSAQGTGAQPVATLMMATNAID
                     GHVDPRLIMDVGVMNGNIPAPMISIDEGDELFLTLTNVGMVMRPDLFEQHTVHFHGYP
                     NASAFYDGVPDASVAINIGGSFTYYYLAPEAGTYMWHCHISPPEHLQMGMVGQLYVRP
                     RQNGVTSGTLLASSQAAQLSIDTKCSSNDMLCSTQVPATDTGAVAASGAMYAFNDGDG
                     TTAYDVEYPIQMHGFDPNFHFVGMTFNPESFVDTKDKFFLLNGRSYPDTVNPAPLSTQ
                     STDGTQHFSQPLSSLINIPLGGKALLRFSDLNVTEYHTLSSLGIPMHVVGYNARLLRD
                     QDGNNTDYFTNSISLAGGESADVILDAHDTNAYPAGSVFYLYTPQLDHLANDAENFGG
                     MMTEVHVLGCTAANFNPQTKQCK"
BASE COUNT          783 a         1773 c         2092 g          942 t
ORIGIN      
        1 agcgcagggt tggtcgagtt gacctggcac ttcgggtcga tccagaacgt gcggtcctcc
       61 tcgatgatcc agcggtagtc ggtgatcgcc ttggtcgggt cgacggcgtc gacgacgttg
      121 atggtcaggc ccgagccggc ctggaaggcg acgatcgccg aggacgacga cgtgctgccc
      181 tggacattgg tcacggtgaa ggggaccagc gcgcaggtcg caccggcggg caacgcgccc
      241 ttggtcgcat cccagccgct gcagttgccc gctgccccgg ggccggccac ggagaacgag
      301 ccgtccgcgt tcacgccggc accggatgcc gtcaacgcga ggccggcggg attggtgacg
      361 ccggacagga cccccggcgg cgtcgagaca taacccgagg cgacgttgct gacgaactgc
      421 accggattgg ccttcaggcc ggtgtcgaat ccgcacccag cgacgctgga cgtggcgtcg
      481 cactcggtga tggtcacggt cttggcgagc gccaggttgc cgttgggcag gtactggaac
      541 gtgccgccgc agctcgcggc gctcgagggc acgtaggtga aggtgccgtt ggacagcagc
      601 gtcagcgtcg tggcgctgag gtcgccggtc atcgcgccga gcgcgacgcc gttgacgccg
      661 gtcgcgccag ccagcaggcc gaggccgggg ttggacaccg ccagcgtctg gccggcggaa
      721 cagtagtagg tcttgtcgtt cgcggacgcg ctcgtgttca gcgcggccga gccgacgccg
      781 ctggcggccc cgccattgac gctgatgtag gcctgcatgc cgccgtcgcg cgcgttgccg
      841 gtcgacaggc tcagctggcg gtcgaacacc gggtaggccg acgcgacgta ggcgccgctc
      901 gcgaccgccg gagcgatcgt gacgtcatag gtcttgcccg cggtcagcag gacggtgtcc
      961 tggatgcggg tcgggccgtg cagcgcgttg ccgtcctcgg ccagcagcgt catgggtgcg
     1021 ccgaccacgc tgggcacgtg catgcgcagg ccggcgttgg ccaggcgcag cagcacggtg
     1081 cctgccgtcg gcgtccccgt cgggcctgcg aacatggacg cggccatgtt ggtgcggtcg
     1141 aacgcggtgc cgttgatcag gtagtacagc ggcgagtagt tgacgatggg cggatagcag
     1201 gagtgcaccg tcggatcgcc gcacatgccc ggctggccgt tccagaccgc gcgcggatcg
     1261 aagcccgccg tgcccaccgc cttggtgacg ccgtcgttga actccgggtc gatctcgctg
     1321 aacagcaggg gcacgtcctt gtcgatcgca ttgccgtagg ccgtcgtggc cccggccgga
     1381 tccgtcacca cgaggatcgc gtacaggccc atcgtgtgct ggatcgatgt ctccgtgccc
     1441 gagtgcagga cgtaggtgcc ggggcgcagg ttggcccaga ccaggctgcc cgtcgtgccg
     1501 ttggccacct cggaggcgag cgaacgcacg cggtcgacct gcggcggcgg cacgaaggtc
     1561 gggctgccgt cgctgctgga gagcacgccg atgtcgatcg tcgcggtcgc gggcacgcag
     1621 gtcgcggacg gcgtgcacgg cggcggtgcg atgctcacgg tcagcgagtc ggcggcggag
     1681 tagccgctgc ccgggttgct gaccacgacg cgggtgaccg cgccgttgcc aacgtaggcg
     1741 accgccgtcg cgccggcgcc gccgtgggcg ttggcgttcg tgatggtcac caccggcggc
     1801 gcgttgtagc cggcacccga ggcaccgagc gtgatggccg acgtcgccgg atcgatcccg
     1861 ccgagcgtgc cgggccaggt ggtgccctgc aggccgtggg tcgggctcgg cgcggtggtg
     1921 cggctgccgc cgagtccgcc gccgacctgg ccgtcgatga tgacggaggt gggcagcccg
     1981 gcgggcagct ggttggcgag gttgatcgtc agctggccgg ccggcacggt gatcagcggc
     2041 ggctgccagg tgatcccgtt ctgcgccgcg ccgttggccg ccgtgcaggt cgcgccggtc
     2101 gacggcgcgg acgccgggag cacgtcgccg caggtgtagc cccacatcgg gacggtgcgc
     2161 ccgtcgggca gcgtcgtcgt gctgggcgat gcctgcaagg tgacggtgcc ggcagcgacc
     2221 gccgaaccag ccattgcagc ggacaaagcg atggccgctg cggcccgcag catcatcgac
     2281 ttgaagagat tcgagtccat gccagtctcc ctcgcctcta gttcgcttcg atgatctgga
     2341 agacccatgg atccaccagc atcatcatca tcattccgcc agggaagatg ttgttcgtcg
     2401 tgatctcgcg ctcgttatgc gagtgccaca tgaatgcgaa gcctgcctcc gtgctcgggc
     2461 tgttcgcgat ggtcccgaac ggcgggatcg gcgtgttgcc cacggagcgg gcgttggcgt
     2521 ccgggcccag gtagggactg ccggagtacc acgcgccgtt ggtcaggatg ttcggatccg
     2581 gcagggtcac cgggccgccc ccggcgaccg acccgaaggg atgcgcctcg agcggcttca
     2641 tgtggtcctc gcaccattcg aagtagttcg gcgcggtgaa cggcgcgggc gggttgctgc
     2701 ccgaggtgta gtagccgttc ttgtcaggcg tgcacggcgc gccgccattg ccttccggcg
     2761 agccgtcgag cgcggcgttg gcggtgtggc cgtagacgtc ccagttcagg cccttgccgg
     2821 tgtactggaa gatgccgtcg atcgtctggc ccggcgtcgt gctggtggtg aacagcagcg
     2881 gcccggcgag gttgcccttc gggctcagca gcatgttgcc gtcgcgcgcc agcacgcgca
     2941 cgtggttggc atgctcgtgc agcgggtgct gcatccggcc ctggccgatg atgcgcagga
     3001 gcacgagttc gcccgggtgc atgtgcgggt tgccgttgta cggctgcaat gggtattgcg
     3061 aggcgtagtt cggatccatg tcgtcgggca tcgagcgtcc gttgaccatg aagtaagccg
     3121 ggtggtacgg ctcggtgtcg atcaccatgc agccggccgg gttggcgcag gccaggtcct
     3181 tctgcgcctg cgcctcgttg tggatgcgga tgtccatctc ggacagctgg atcaggtact
     3241 cgcggtcgta gcacgcttcc ggatggtcgt aggcggccgc ggcgaggcgg tagtccggtg
     3301 ctccgttggg cagcgtcgcg ccggccgagg cgggactcgc ggcgaagcac gtgttgggca
     3361 acccgccgcc ggcggcctgc ggcgtgtagg acgagggcac ggcgccgttg agcggcgcgt
     3421 aggtgttgct cgtcacgtgc gcgggcagca cgatcagcgc gccgaacatc cccatctcga
     3481 tctgcaggtc gccctgcgtg ccgctgtagt aggaatgcgt cccgggggta tcggccacga
     3541 acgtgtacgt cactgcgccg ccgggcacgg cctcctgcgc gagcacgccg ggcgagccgc
     3601 cggtgctcgt cacgtggaag cccgggaaca ggatcgacgt gctgcccgcc gcggccggca
     3661 ggccgttgtg gagcgtcacg gagaccgtgt cgccctggtt gacgaccagg gtcggcccgg
     3721 gcagttgcat ctggcccgcc gggcaggcgg cgttggcgat cgtcgccggg acgaagctcg
     3781 ccggggcgct gttgcaaccg tagccccagg tgtagaccga ggccccgtcc ggctgcgaga
     3841 tgtagccgtc cttggcgacc aggtcgaagg cgggcccggt gatgcccggc gacgcggcgt
     3901 gcgcgccgct cgccagcgtc gcccacgccg ccacggccgc gacgcgccgc agttgcatca
     3961 ggaatccatg gagattgcgc atgtccctct ccctacttgc actgcttggt ctgggggttg
     4021 aagttggccg ccgtgcatcc gagcacgtgg acctcggtca tcattccgcc gaagttctcg
     4081 gcatcgttgg ccaggtgatc cagttgcggc gtgtacaggt agaacacgct gcccgccgga
     4141 tatgcgttgg tgtcgtgggc gtccaggatc acgtccgccg actcgccgcc ggccagcgag
     4201 atggagttgg tgaagtagtc ggtgttgttg ccgtcctggt cgcgcagcag gcgcgcgttg
     4261 tagccgacga cgtgcatcgg gatgccgagc gacgacagcg tgtggtactc ggtgacgttg
     4321 aggtcggaga agcgcaacag cgccttgccg cccagcggga tgttgatcag cgaggacagc
     4381 ggctgcgaga agtgctgcgt gccgtcggtc gactgcgtgc tcagcggcgc ggggttgacg
     4441 gtgtccgggt agctgcggcc attgagcagg aagaacttgt ccttggtgtc gacgaaggac
     4501 tcggggttga acgtcatgcc cacgaagtgg aagttcgggt cgaagccgtg catctggatc
     4561 ggatactcca cgtcgtatgc cgtggtgccg tcgccgtcgt tgaacgcgta catcgcgccg
     4621 ctggccgcca ccgcgccggt gtcggtcgcg ggcacctgcg tggagcagag catgtcgttc
     4681 gacgagcact tcgtgtcgat cgacagctgg gccgcctgcg aggaggccag cagcgtgccg
     4741 ctggtcacgc cgttctgccg cggccgcacg tacagctgtc cgaccatgcc catctgcagg
     4801 tgctccggcg gcgagatgtg gcagtgccac atgtacgtgc cggcctcggg cgccaggtag
     4861 tagtaggtga agctgccgcc gatgttgatg gccaccgagg cgtcgggcac gccgtcgtag
     4921 aaggccgagg cgttcgggta gccgtggaag tgcacggtgt gctgctcgaa caggtcgggg
     4981 cgcatcacca tgccgacgtt ggtcagcgtc aggaacagct cgtcgccttc gtcgatcgag
     5041 atcatcggcg ccgggatgtt gccgttcatc acgccgacgt ccatgatcag ccgcgggtcg
     5101 acgtggccgt cgatcgcatt ggtggccatc atcagcgtgg cgaccggctg cgcgccggtt
     5161 ccctgggcgc tgacgatcga gacgtccggc accgaggtgt agttcacgcc cgggttggtg
     5221 atgctgatgg tgaggatcgc gccggtgccg ctcaccgtgg cgaccgcgcc caccgccccc
     5281 gagccgccgc cgccggtgaa gacgacggca tcgccgacct tgtagcgcgt gccggtgatg
     5341 ggcaggtcgg tggccggatc gatgttgaac gtgacgccgg ccacctgctc ggcgttgatg
     5401 tcgggcgcca ggccgatcgc gccgttgaac gcgtagtccg cgctgtcggg cgcgccgatc
     5461 ttgaccacgt ccagcacgtt gccctgcgcg tcgaggttgc tctggttgaa ctccgacgcg
     5521 gagacggtgc cgggcaggcc gtgaacgatg ttgcccaggc ccgagagcgg gccgaacgag
     5581 aacatgtagg
//
LOCUS       DATCRO010000299         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_801883, whole genome shotgun sequence.
ACCESSION   DATCRO010000299 DATCRO010000000
VERSION     DATCRO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801883"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1478
                     /locus_tag="VIN75_08075"
     CDS             168..1478
                     /locus_tag="VIN75_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974160.1"
                     /translation="MNSITAPSQGQPGRIDSVADAVYRKVAWRLIPLLFLCYIAAYLD
                     RVNVAFAKLQMLQALNLSEAVYGLGASIFFIGYFIFEIPSNMMLHRVGAGRWIARIMV
                     SWAVLSAAMMFVNSATSFYIVRFLLGVAEAGFFPGIILYLTYWFPAARRGRATSLFLA
                     AVAVAGLVGNPVSGWILHSLNGTFGLQGWQWLFVLEALPSLVLGVLAWFHLEDRVKNA
                     QWLTIEERALVARDIEAEESLKSHGKLAKVLADGKVWLLAFVYFAIVSGLYGVTFWLP
                     TIIKELGIADPLQVGLVGAVPWAFGMVAMYVMARNADRRNEYRWHTAIPCVVGAAGLL
                     VSVAFHADVVLAMAGLTICTMGIMAALPIFWSNPTAILGGAAAAAGIAFINSMGNLAG
                     FAMPSIIGLIKEKLHSIDAGLEMLAALMVLGAAAVLALRKPASH"
     gene            1573..2106
                     /locus_tag="VIN75_08080"
     CDS             1573..2106
                     /locus_tag="VIN75_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEY8974161.1"
                     /translation="MSLVFPAVHDARLEADTREPVTLLAPDQHRDAADAAYEDAELLL
                     TSILPDARIEHVGATAVAGARSRGGVDVCVAVPRGAFDEALAVLVEAGCLQRSQEDAA
                     DRHAALAMPSAALPVTLQLIETGSVHESLMRVRDALRADAALMGRLNAAKAGAAGQGD
                     AAYAAAKARFFEALIKR"
     gene            complement(2150..2515)
                     /gene="speD"
                     /locus_tag="VIN75_08085"
     CDS             complement(2150..2515)
                     /gene="speD"
                     /locus_tag="VIN75_08085"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY8974162.1"
                     /translation="MQGLHLIADLRDCAGPTPLDDEAALAALCLDAVARAGLRAVGSL
                     FHPFTGPDGSPQGVTGVVLLVESHLAVHTWPELGSVTADVYVCNVGADNSARAEAVMA
                     RLVEAFGPREPRVQRVLRG"
     gene            2615..3499
                     /locus_tag="VIN75_08090"
     CDS             2615..3499
                     /locus_tag="VIN75_08090"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEY8974163.1"
                     /translation="MQHDLNADLHSHSTASDGTLDSSQLAARAKANGVELWALTDHDE
                     LAGLPAARAAAERAGLPFLDGVEISVTFAGTTVHIVGLGIDPANEELAAGIAQVRAGR
                     EERARQMGDGLAQVGIKGAYEGAMKFAPNPNLVSRTHFGRFLVASGISPDMRSVFGRY
                     LTEGKPGYVEHRWARLGDAVRWIRAAGGVAVIAHPGRYKFTPTEEYALFSEFQAHGGE
                     GVEVVTGSHHAGDIAKYTDMALEFGMLASRGSDFHDPQESRLDLGLRPTPLALSLRLT
                     PVWSRWSKDDLRPKRPAS"
     gene            3608..4240
                     /locus_tag="VIN75_08095"
     CDS             3608..4240
                     /locus_tag="VIN75_08095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8974164.1"
                     /translation="MAQFFDVHPDNPQLRLLRQAAQILHDGGLAAVPTDSSYAFVCHL
                     DDKDAVEALRRVRGVDEKHHLTLLCRDLSELANYARVDNRQYRLLKNATPGPFTFILD
                     ATKEVPRRVSHPSRRTIGLRVPDHKVLQALLEELGQPLLATTLIPPGETQPLNDTDEI
                     RRRFEKSIQAIVCAGACPMEPTTVIDLTGDDPVLVREGRGDPALLGLVTS"
     gene            complement(4282..4632)
                     /locus_tag="VIN75_08100"
     CDS             complement(4282..4632)
                     /locus_tag="VIN75_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8974165.1"
                     /translation="MSEIKRFDVGQRMSEMAVHNGVAYLAGQVASDASLDAQGQTANV
                     LAQIDALLARAGTDKSRILMAQIYLADVADFAAMNAAWDAWVAGGNTPPRATVEARLA
                     KPEWKVEIVVKAAV"
     gene            complement(4658..5287)
                     /gene="plsY"
                     /locus_tag="VIN75_08105"
     CDS             complement(4658..5287)
                     /gene="plsY"
                     /locus_tag="VIN75_08105"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY8974166.1"
                     /translation="MFWSVCVVLLAYLLGSLSFAVIVSRAMRLDDPRSYGSGNPGATN
                     VLRSGNKLAAILTLLLDALKGVVAVLIARFLAERLGYGDGVVALAGLASFLGHLAPIF
                     FRFQGGKGVATAAGVLLALNWVTGLACLGTWLAIAVLTRYSSLAALVTAVLAPVYFHF
                     MHGDSQVVGAVAVMSVLLIWRHRSNITKLLNGTESKLGSKAGKPSDSAT"
BASE COUNT          810 a         1984 c         1909 g          807 t
ORIGIN      
        1 gtcttgaacg ttgcggggtc aggtcttgaa tcgtgcactc cactgcgcga ttcaagacct
       61 gaccccgcaa cgttcaagac ctgaccccga tggaatgggt gtttccccgc tgcccacggc
      121 gcgcggccgg tcgcaggatc gcggcgtcac aggacaggag acgctcgatg aacagcatca
      181 ccgcgccctc gcaggggcag ccgggccgga tcgattcggt cgccgacgcg gtctaccgca
      241 aggtcgcgtg gcggctgatc ccgctgctgt tcctgtgcta catcgcggcc tacctcgatc
      301 gcgtcaacgt cgccttcgcg aagctgcaga tgctgcaggc gctcaatctc agcgaggcgg
      361 tgtacggcct gggcgcgagc atcttcttca tcggctactt catcttcgag atcccgagca
      421 acatgatgct gcacagggtg ggcgcgggcc gctggatcgc gcgcatcatg gtcagctggg
      481 cggtcctgtc ggccgcgatg atgttcgtga actcggcgac gagcttctac atcgtccggt
      541 tcctgctcgg cgtcgcggag gccggcttct ttcccggcat catcctgtac ctgacctact
      601 ggttcccggc ggcgcggcgc ggacgggcga cctcgctgtt cctggcggcc gtcgcggtcg
      661 ccgggctggt cggcaacccg gtgtcgggct ggatcctgca ttcgctcaac gggaccttcg
      721 gcctgcaggg ctggcagtgg ctgttcgtgc tggaggccct gccctctctg gtgctgggcg
      781 tgctggcctg gttccatctc gaggatcgcg tgaagaacgc gcagtggctg accatcgagg
      841 agcgcgccct cgtcgcgcgc gacatcgagg ccgaggaatc gctgaagtcg cacggcaagc
      901 tcgccaaggt gctggccgac ggcaaggtct ggctgctcgc gttcgtctac ttcgcgatcg
      961 tctcgggcct gtatggcgtg acgttctggc tgcccaccat catcaaggaa ctcggcatcg
     1021 ccgatccgct ccaggtaggc ctggtcggcg cggtgccgtg ggcgttcggc atggtcgcga
     1081 tgtacgtgat ggcgcgcaac gccgatcgcc gcaacgagta ccgctggcac acggcgatcc
     1141 cgtgcgtcgt cggcgccgcg gggctgctgg tcagcgtcgc cttccacgcc gacgtggtgc
     1201 tggccatggc gggcctgacg atctgcacga tgggcatcat ggcggcgctg ccgatcttct
     1261 ggagcaaccc gaccgccatc ctcgggggcg ccgcggcggc cgcgggcatc gccttcatca
     1321 actcgatggg caacctcgcg ggcttcgcga tgccgtcgat catcggcctg atcaaggaga
     1381 agctgcactc catcgatgcc ggcctcgaga tgctggccgc actgatggtg ctgggcgccg
     1441 ccgccgtgct cgcgctgcgc aagccggcgt cgcattgacg agacgccccg ggtcagcgcc
     1501 gatgccgggc cgtcgcgcgc ggggcagaat ccgcgccatc ccagcctggc gcgccctgcg
     1561 ccgaatccat ccatgagcct ggtcttcccc gccgtgcatg acgcgcgcct cgaagccgac
     1621 acccgcgagc cggtgacgct gctcgcgccc gaccagcacc gggatgccgc cgacgcagcg
     1681 tacgaggacg ccgagctgct gctgacgtcc atcctgcccg atgcgcgcat cgagcacgtg
     1741 ggcgccaccg ccgtcgccgg cgcgcgctcg cgcgggggcg tcgacgtctg cgtcgcggtg
     1801 ccgcgcggcg cgttcgacga ggcgctcgcc gtgctcgtcg aggccggctg cctgcagcgc
     1861 tcgcaggagg acgccgccga ccgccacgcc gcgctggcca tgccgtcggc agccctgccc
     1921 gtgacgctgc agctgatcga gaccggctcg gtccacgagt cgctgatgcg ggtgcgcgat
     1981 gcgctgcggg cggacgccgc cctgatgggg cgcctgaacg ccgccaaggc cggcgccgcg
     2041 ggccagggcg acgccgccta cgcggccgcg aaggcgcgct tcttcgaggc gctgatcaag
     2101 cgctgatcga tcgctgccga gtcccgcgag gcgggcatgc cgcccggcgc tacccgcgca
     2161 gcacgcgctg cacgcgcggc tcgcgcgggc cgaaggcctc caccagccgc gccatgacgg
     2221 cctccgcgcg cgccgaattg tcggcgccga cgttgcacac gtagacgtcc gccgtcacgc
     2281 tgcccagctc gggccaggtg tgcacggcca ggtgcgactc caccagcagc accacgccag
     2341 tgaccccctg cgggctgccg tcgggaccgg tgaacggatg gaacaggctg ccgaccgcgc
     2401 gcaggccggc gcgggcgacc gcgtccaggc acagcgcggc gagcgcggct tcgtcgtcca
     2461 gcggcgtggg gcccgcgcag tcgcgcaggt cggcgatgag atggagtcct tgcatcgggc
     2521 aacggtgacg cgcatgaggc gtgcgcgccg caatggtagc cgcgcattca ggcggcaccg
     2581 acggggacac cgcaccctcg ctaaaatcgg cgcagtgcag cacgatctca acgccgacct
     2641 ccacagccat tcgaccgcct cggacggcac gctggactcg agccagctcg cggcgcgggc
     2701 caaggccaac ggcgtcgagc tgtgggcgct gaccgaccac gacgagctcg ccggcctgcc
     2761 cgccgcgcgc gccgcggccg agcgcgccgg cctgcccttt ctcgacggcg tcgagatctc
     2821 cgtcaccttc gccggcacca cggtgcacat cgtcggcctg ggcatcgatc cggccaacga
     2881 ggagctggcc gccggcatcg cgcaggtgcg cgccggccgc gaggagcgcg ccaggcagat
     2941 gggcgacggc ctcgcgcagg tcggcatcaa gggcgcctac gaaggcgcga tgaagttcgc
     3001 accgaacccc aatctcgtct cgcgcacgca cttcggccgc ttcctggtgg ccagcggcat
     3061 ctcgcccgac atgcgcagcg tgttcggccg ctacctcacc gagggcaagc cgggctacgt
     3121 cgagcaccgc tgggccaggc tgggcgacgc cgtgcgctgg atccgcgcgg ccggtggcgt
     3181 ggccgtgatc gcgcatccgg gccgctacaa gttcacgccg accgaggaat acgcgctgtt
     3241 ctccgaattc caggcgcacg gcggcgaggg cgtcgaggtc gtcaccggca gccaccatgc
     3301 cggcgacatc gccaagtaca ccgacatggc gctcgagttc gggatgctcg cctcgcgcgg
     3361 cagcgacttc cacgatccgc aggagagccg cctcgacctc ggcctgcggc cgacgccgct
     3421 cgcgctgtcg ctgcgcctga cgccggtgtg gtcgcgctgg agcaaggacg acctgcgccc
     3481 gaagaggccc gcgtcgtgag cgcgcgctgg cccgtccacg ccatccgctg cagggcgccg
     3541 cggtcgacgt gctgcccgga taggcactgt cgtcgccggg gaggcgtcgc gcgttgacaa
     3601 tgcacccatg gcccagttct tcgacgttca ccccgacaac ccgcagctgc ggctgctgag
     3661 gcaggccgcg cagatcctgc acgacggcgg cctggcggcg gtgcccaccg attcgagcta
     3721 cgccttcgtc tgccacctgg acgacaagga cgccgtcgag gcgctgcgcc gcgtgcgcgg
     3781 cgtcgacgag aagcaccacc tgacgctgct gtgccgcgac ctgtcggagc tcgcgaacta
     3841 cgcgcgcgtc gacaatcggc agtaccgctt gctcaagaac gcgacgccgg gtccgttcac
     3901 gttcatcctc gacgcgacca aggaagtacc gcgccgggta tcgcacccgt cgcgccgcac
     3961 catcggcctg cgcgtgcccg accacaaggt gctgcaggcc ctgctcgagg aactgggcca
     4021 gccgctgctg gccaccacgc tgatcccgcc gggcgagacg cagccgctga acgacaccga
     4081 cgagatccgc cggcgcttcg agaagagcat ccaggccatc gtctgcgcgg gcgcctgccc
     4141 gatggagccg accacggtga tcgacctcac cggcgacgat ccggtgctgg tgcgcgaggg
     4201 ccggggcgac ccggccctgc tcggcctcgt gacgagctag gagtccgcgc ggcttctgcc
     4261 gcgcagactc ctagtctcgc ctcagaccgc ggctttcacg acgatctcga ccttccattc
     4321 cggcttggcg agcctggcct cgaccgtggc gcgcgggggc gtgttgccgc cggcgaccca
     4381 ggcatcccac gcggcgttca tcgccgcgaa gtcggccacg tcggccaggt agatctgcgc
     4441 catcaggatg cgcgacttgt cggtgccggc gcgcgccagc agggcgtcga tctgcgccag
     4501 gacgttggcc gtctggccct gcgcgtcgag gctggcgtcg gaggccacct ggccggccag
     4561 gtaggcgacg ccgttgtgca cggccatctc ggacatgcgc tggccgacgt cgaatcgctt
     4621 gatttcgctc atgaagattc tctcggggtg atgttgttca ggtcgcggag tcggaaggct
     4681 tgccggcctt gctgcccagc ttggattcgg tgccgttcag cagcttggtg atgttgctgc
     4741 ggtgacgcca gatcagcagc acgctcatga cggccaccgc gccgacgact tgtgagtcgc
     4801 cgtgcatgaa atggaaatag acgggggcca gcaccgccgt caccagcgcg gcgagcgacg
     4861 agtagcgcgt gagcacggcg atcgcgagcc aggtgcccag gcaggcgagg cccgtcaccc
     4921 agttcagcgc cagcagcacg ccggccgccg tggccacgcc cttgccgccc tggaagcgga
     4981 agaagatcgg ggccaggtgg ccgaggaagg acgcgaggcc cgccagcgcc accaccccgt
     5041 cgccatagcc caggcgctcg gccaggaagc gcgcgatcag caccgccacg acgcccttga
     5101 gcgcgtccag cagcagcgtg aggatggccg ccagcttgtt gccgctgcgc agcacgttgg
     5161 tggcgccggg gttgcccgag ccgtaggagc gcggatcgtc caggcgcatc gccctggaga
     5221 cgatcaccgc gaagctcagc gacccgagca ggtacgcgag cagcaccaca cacaccgacc
     5281 agaacatcgc caccgacggc accctcgttg aatgcgaaag cggcgatttt acgtgcgcgc
     5341 tacacggcgc ccagcgcccg cacctgctcc accagcgcca gcggcttctg cacccagccc
     5401 ttgcggaagg ccaggaactc gtcgagcggg cacgccggcg agacgagcca gccctggatc
     5461 tcgtcgcagc cgacggccgt cacggcgctc agctgcgcgg gcgattcgac
//
LOCUS       DATCRO010000300        10327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_25532, whole genome shotgun sequence.
ACCESSION   DATCRO010000300 DATCRO010000000
VERSION     DATCRO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25532"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2015
                     /locus_tag="VIN75_08110"
     CDS             249..2015
                     /locus_tag="VIN75_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974167.1"
                     /translation="MFKNLLNRILPSPAPAPAPAPAPTPTPAPAARETTANDIAAADA
                     LIVQGNALEDAGMLPRAEALYRAAVEKAPGHARAHLNLGIVLAAQGDDDGAIAAYERV
                     LAIDPAHPFGNYNYARLLVVRGDNDRAETLAAQALRARPDFPQATELLARILRDQGFA
                     REALVPLRSIIERDPANWLHRSFELLVMNSAGDDDADAMFRRHVQFGAGLEQAVPPRF
                     AHHPERGDPARRLRVGYLGSDFLLHPVAFFLIPVLEHHDRTQVEVFCYSSETRRDGMT
                     DRIQDTCDHWREVAALSDDALADLIHADGIDVLVDLIGHTGIPRLGVFCQRPAPVQVE
                     WLGYLNTTGLTRMDFRLSDERADPPAISQPRHTERLVSMPASQWCYRAMVDQDIEPAP
                     PFERNGHLTFGSFNAAIKITPAACRRWAQVLLRVPGSRLVIGDINSGRKRAAIRRDIE
                     GEGVAADRVEFMPRVGFDKYLGLYNAVDITFDTFPYGGGTTTFDSLWMGVPVVAAVGD
                     TPVSRSAASILAALGLDDWIAPDADRFVDLAVARASDLEALRTLRRDLRPRLQASPLT
                     DLPRYTRDLEAAWRRMWLDRTA"
     gene            2056..4014
                     /locus_tag="VIN75_08115"
     CDS             2056..4014
                     /locus_tag="VIN75_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974168.1"
                     /translation="MLKNLLNRILSSPTAPTPAPAPSQVEAPAPVPVVPDDVAGADAL
                     IAQGNALEDAGQLARAEALYREAVARAPGHARAHLNLGIALAAAGDEDGAGAAYERVL
                     AIDAAHPFGNYNYARLALVRGDLARAETLIAAAVRAKPDFAQALVVQSNVLDARGQTA
                     GAVAAIEAALRLRDDDAGAWYNLGTLLMRQARADDAEPALRRALELDPGNADSLALLA
                     RVLRDQGFADAALATISPVTAQRPQSWPDRSLELLLTLFAQGLSQDEIFRRHARLGAD
                     LEQAVPARFQHAASPAPKRLRVGYLSADFVLHPVTFFVVPVLEQHDRTQVEVFCYSFG
                     KGSDGVTERIRALSDHWRDVAALSDTQIADRVHADGIDVLVDLAGHTGVPRTGVFAQK
                     PAPVQVGWVGYLGTTGLSRMDYRLCDARTDPLALAQPFHTERLVHLPTSQWCYRPMLV
                     EAIGEAPFERNGHLTFGSFNASLKLSDAVLQRWGEALARLPGSRLRVANINSERKRAA
                     IREALARQGVAGDRVEFLARVPLNKYFDLYNGVDIALDSFPYGGGTTTLDALWMGVPV
                     ATAVGETSVSRSAASVLAEVGLDDWVAPSLEAFVDRVVERASDREALRRLRRELRPRL
                     QASPLTDMPRFVRDLEAAYRAMRGGELR"
     gene            complement(4025..5038)
                     /locus_tag="VIN75_08120"
     CDS             complement(4025..5038)
                     /locus_tag="VIN75_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974169.1"
                     /translation="MARIELATLTRTITADALAHPTDLGPTLARRHSVSRGTMTKQLK
                     RLVDSGWLVRTGVTRPHFKPGVQREVTNEYAIAGLDEHTPWLRDVHPVLQLSPAVARV
                     AQHVFGELLNNAIDHAQATRVTVSVRQTPTHLHILIGDDGLGVFNQLAAKAELPDAQR
                     AALEISKGRLTTQPECHGGRGLFFVARMVDAMNLQSNGQVLQWRHRGESVQFRSHPLN
                     RTGTTVFVSLALDSTVRPTDTYSAFGSEDAPLDFSRTQVPLRLLTDNGLTLDSRAQAR
                     WVVSRLELFATAELDFDGVDDVGPSFIDEVFRVYAKAHPEVALQPTKASERVGKLLKG
                     VMH"
     gene            complement(5347..7911)
                     /locus_tag="VIN75_08125"
     CDS             complement(5347..7911)
                     /locus_tag="VIN75_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974170.1"
                     /translation="MPRPSSYTAVSPATAQLNQAVALHQAGRLEEAAAAYRQFLQQFP
                     GQFDATHLLGVIALQRGDLATAETQITQALASKPKDAAALNNLGTVLLRARRLDEARE
                     QFERAAKAQPTFADAQSNLGNVLRQLGRVAESLVPLKRAYGASPKSAEIADLLGASLL
                     ETGDGRGAVAIFETSVKSRPRDARAWLNLSIACDLAGNPKRALEAADQSLALEPAAPA
                     HGARGRALAALGDRAAARTAFDAGVRGAPRDAVAHHNFGVFLREQGEIDAALTHLREA
                     LRLDPQLAAAHEALVGALVDGGRGDEALRHGQAFGQSHPGSSAALAMQASAQFALGHM
                     EDAVELYARAVGAPGADAATFLGHGNALFGAGDAAAATRQFARAVELDPQNARARFAL
                     AMSHLRPVYDDAAQMDASRKAFTRALGELDAWFTPARAAAGSTAVGSALPFYLAYHPV
                     DVRALLLPYGKLATRLMAAGAPLAGAQTSRASAGAARKLRVGIAAAHVRDHSVWNAIT
                     QGWVRHLDRARIELTIFKLDRASDDETARARRDAAAFVDTPTTLDEWTRAITDARLDV
                     LIYPEIGMHPLTARLAALRLAPVQAVAWGQPLTSALPTMDLFLSAQALEPDGAASHYA
                     EKLVALPHLGVCLQPLQPEEVVPDFAGLGLNPDAPLLLCPGTPFKYTPQGDAALVAIA
                     KGLQARGRGRLVFFLSHRPGMSRQVEQRMRQAFGKAGVDYDRTVAWVPTLTRGRFHGL
                     MRRSTALLDTLGFSGFNTAVQALECDLPVVAFEGPFMRGRLASGPLRHLGLDELVAGT
                     PEAFAAIALRLAEDEAWRAHLEREIAQRRHALFFDQAPVRALEDALFEAAGVARPT"
     gene            complement(7911..8246)
                     /locus_tag="VIN75_08130"
     CDS             complement(7911..8246)
                     /locus_tag="VIN75_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974171.1"
                     /translation="MVFSWFNTSKVDAFVDAEVADLVKRVPPARLEGDGAGARKAAEQ
                     LAKGHDLLLRHAKDFVQREKPNLYQKARIANRMKWALLEARYPKSFVDEFAYALASVV
                     AAANANREV"
     gene            8442..9506
                     /gene="fba"
                     /locus_tag="VIN75_08135"
     CDS             8442..9506
                     /gene="fba"
                     /locus_tag="VIN75_08135"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870050.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8974172.1"
                     /translation="MPVVSMRQLLDHAAENGYGIPAFNVNNLEQVQAVMSAAAEVGAP
                     VILQASAGARKYAGEPFIKHLIQAATESWPQIPLVMHQDHGQNPEVCRGAMDLGFSSV
                     MMDGSLQADGKTIASYDYNVDVTRTVVDMAHARGITVEGELGCLGSLETMKGDKEDGH
                     GTDATMTRDQLLTDPEQAADFVKRTQLDALAIAIGTSHGAYKFTRKPTGDILAIDRIK
                     EIHRRIPTTHLVMHGSSSVPQDLLAIIRQFGGNMKETYGVPVEEIQEAIKHGVRKINI
                     DTDIRLAMTGAIRKYLFENPDKFDPRDYLKPAREAAKAICRQRYVEFGCEGQAGKIKT
                     DTLQVVAAKYASGELAQTVH"
     gene            9682..>10327
                     /locus_tag="VIN75_08140"
     CDS             9682..>10327
                     /locus_tag="VIN75_08140"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8974173.1"
                     /translation="MTAALLTTSIHSLPLLARGKVRDNYAVGDDRILMVATDRLSAFD
                     VILGEPIPGKGALLTQMALFWFGKLEGIVPNHLTGEAPESVVQADEVAQVRERSMLVK
                     RLKPLPIEAVVRGYLAGSGWKEYQASQSVCGVQLPAGLKNASKLPEPIFTPATKAEAG
                     EHDENITFKVASAMIGNELANHVRDVSIALYERAAEYALTKGIIIADTKFEFGLD"
BASE COUNT         1510 a         3737 c         3681 g         1399 t
ORIGIN      
        1 ggcggcgagc gcgaggaagc ggtcgaagcc acggaacccg ctgaatccgg ccgtccaggc
       61 cggattccga cgcacggcaa gccggagagc ggagcccctg cccaccctgt cgcaagcgca
      121 acgccggcca gcgcgaagcg acacaccggt ctcaagccca acgccacccg ccgcgaagcg
      181 acacgcgccc catcgcaaga gaaggagatt cccggcccac aatgcagcat ccgccggacg
      241 ccccagccat gttcaagaac ctgctgaacc gaatcctgcc gtcacccgca cccgcacccg
      301 cacccgcacc ggcaccgaca ccgacaccgg cacccgcagc gcgcgagacg accgccaacg
      361 acatcgccgc cgccgacgcg ctgatcgtgc agggcaacgc actggaagat gcgggcatgc
      421 tgccgcgcgc cgaggcgctg taccgcgcgg cggtcgagaa ggcgccaggg catgcgcggg
      481 cgcacctgaa cctgggcatc gtgctcgcgg cgcaaggcga cgacgacggc gcgattgccg
      541 cctacgaacg cgtgctcgcg atcgacccgg cccacccgtt cggcaactac aactacgcgc
      601 ggctgctggt cgtgcgcggc gacaacgacc gggccgagac gctggccgcg caggcgctgc
      661 gtgccaggcc cgacttcccg caggccaccg aactgctcgc ccgcatcctg cgcgaccagg
      721 gcttcgcgcg cgaggccctc gtgccgctgc gcagcatcat cgagcgcgac cccgccaact
      781 ggctgcatcg ctcgttcgag ctgctggtca tgaattccgc gggcgacgac gatgccgacg
      841 cgatgttccg gcgccacgtg caattcggcg ccggcctgga gcaggccgtg ccgccgcgtt
      901 tcgcgcacca tcccgagcgg ggcgacccgg cgcggcggct gcgcgtgggc tacctcgggt
      961 cggacttcct gctgcacccg gtcgcgttct tcctgattcc cgtgctcgag caccacgatc
     1021 gcacgcaggt cgaggtgttc tgctattcgt ccgagaccag gcgcgacggc atgaccgacc
     1081 ggatccagga cacctgcgac cactggcgcg aggtcgccgc gctgtcggac gacgcgctgg
     1141 ccgacctgat ccatgccgac ggcatcgacg tgctggtcga cctgatcggc cacaccggca
     1201 tcccgcgcct gggcgtgttc tgccagcgtc ccgcgccggt gcaggtggag tggctgggct
     1261 acctcaacac gaccggcctg acccgcatgg acttccgcct gagcgacgag cgcgccgatc
     1321 cgcccgccat ctcgcagccg cgccacaccg agcggctggt ctcgatgccc gcgagccagt
     1381 ggtgctaccg cgcgatggtg gaccaggaca tcgagcccgc gcccccgttc gagcgcaacg
     1441 gccacctgac cttcggctcg ttcaacgcgg ccatcaagat cacgcccgca gcctgccggc
     1501 gctgggcgca ggtgctgctg cgggtgcccg gctcgcggct ggtcatcggc gacatcaact
     1561 ccgggcgcaa gcgtgcggcc atccggcgcg acatcgaggg cgagggcgtg gccgccgatc
     1621 gcgtcgagtt catgccgcgc gtcggcttcg acaagtatct cggcctgtac aacgcggtcg
     1681 acatcacgtt cgacaccttc ccgtacggcg gtggcaccac gacgttcgac tcgctgtgga
     1741 tgggcgtgcc cgtggtggcg gcggtcggcg acacgcccgt ctcgcgcagc gcggccagca
     1801 tcctcgcggc gctcgggctg gacgactgga tcgcgccgga cgccgaccgc ttcgtggacc
     1861 tcgccgtggc gcgcgcgtcc gacctcgagg ccctgcgcac cctgcgacgc gacctgcgcc
     1921 cgcgcctgca ggcatcgccg ctgaccgacc tgccgcgcta tacccgcgac ctcgaggcgg
     1981 cctggcgccg gatgtggctg gacaggacgg cctgacaaac cggccgacaa tgcgccacgg
     2041 agacgccgcc gccccatgct caagaacctg ctgaaccgaa tcctgtcgtc gcccaccgcg
     2101 ccgacgcctg cgcccgcacc ctcgcaggtg gaggcgcccg cgccggtgcc cgtggtgcct
     2161 gacgacgtcg ccggcgccga cgcgctcatc gcgcagggca acgcgctcga ggacgccggc
     2221 cagctcgcgc gcgccgaggc gctgtaccgc gaggccgtgg ccagggcgcc ggggcatgcg
     2281 cgggcgcacc tgaacctggg catcgcgctg gcggccgccg gcgatgagga cggcgccggc
     2341 gccgcctacg agcgcgtgct ggcgatcgac gcggcgcatc cgttcggcaa ctacaactac
     2401 gcgcggctcg cgctggtgcg cggcgacctg gcgcgcgcgg agacgttgat cgccgccgcc
     2461 gtgcgcgcga agccggattt cgcccaggcg ctggtcgtgc agtccaacgt gctggacgcg
     2521 cgcggccaga ctgccggggc cgtcgccgcg atcgaggcgg cgctgcgcct gcgcgacgac
     2581 gacgccggtg cctggtacaa cctgggcacg ctgctgatgc gccaggcgcg cgcggacgac
     2641 gccgagccgg cgttgcggcg ggcgctcgaa ctcgatcccg gcaacgccga ctcgctcgcg
     2701 ctgctggccc gcgtgctgcg cgaccagggc ttcgcggacg cggcgctcgc gacgatctcg
     2761 ccggtcaccg cgcagcggcc gcaatcgtgg ccggaccgat cgctcgagct gctgctgacg
     2821 ctgttcgcgc aaggcctgtc gcaggacgag atcttccgcc gccacgcgcg cctgggcgcc
     2881 gacctcgagc aggcggtgcc cgcgcgcttc cagcacgccg cgtcgcccgc cccgaagcgc
     2941 ttgcgcgtgg gctacctctc ggccgacttc gtgctgcacc cggtcacttt cttcgtggtg
     3001 ccggtgctcg agcagcacga tcgcacgcag gtcgaggtgt tctgctactc gttcggcaag
     3061 ggcagcgacg gcgtcaccga gcgcatccgc gccctgagcg accactggcg cgacgtggcg
     3121 gccctgagcg acacgcagat cgccgaccgc gtccacgccg acggcatcga cgtgctcgtc
     3181 gacctcgccg gccacaccgg cgtgccgcgc acgggcgtgt tcgcgcagaa gccggcgccg
     3241 gtgcaggtgg gctgggtcgg ctacctcggc accacgggcc tgtcgcgcat ggactaccgc
     3301 ctgtgcgacg cgcgcaccga cccgctcgcg ctggcgcagc ccttccacac ggagcggctg
     3361 gtgcaccttc cgacgagcca gtggtgctac cggccgatgc tggtcgaggc gatcggcgag
     3421 gcgccgttcg agcgcaacgg ccacctgacg ttcggctcgt tcaacgcgtc gctcaagctg
     3481 tcggacgccg tgctgcagcg gtggggcgag gcgctggcgc gcctgcccgg ctcgcgcctg
     3541 cgggtggcca acatcaactc cgagcgcaag cgtgcggcca tccgcgaggc gctcgcgcgc
     3601 cagggcgtgg ccggcgaccg cgtcgaattc ctggcccgcg tgcccctgaa caagtacttc
     3661 gacctgtaca acggcgtcga catcgccctg gacagcttcc cgtacggcgg cggcaccacc
     3721 acgctggacg cgctgtggat gggcgtgccc gtggccacgg cggtcggcga gacctcggtg
     3781 tcgcgcagcg cggccagcgt gctggcggag gtgggcctgg acgactgggt cgcgccgtcg
     3841 ctggaggcct tcgtcgaccg ggtcgtcgaa cgcgcgtccg accgcgaggc gctgcgacgg
     3901 ctgcgccgcg agctgcgccc gcgcctgcag gcctcgccgc tcaccgacat gccgcgcttc
     3961 gtgcgcgacc tcgaggcggc gtaccgcgcg atgcgcggcg gcgagctgcg ctgagccgcg
     4021 cggctcagtg catcacgccc ttgagcagct tgcccacgcg ttcgctggcc ttggtcggct
     4081 gcagcgccac ttccgggtgc gccttggcgt agacgcggaa gacctcgtcg atgaagctgg
     4141 gtccgacgtc gtcgacgccg tcgaagtcga gttcggcggt ggcgaacagc tccaggcgcg
     4201 acacgaccca gcgggcctgc gcgcgcgagt ccagcgtcag gccgttgtcg gtcagcaggc
     4261 gcagcggcac ctgcgtgcgg ctgaagtcca gcggcgcatc ctcggagccg aacgcgctgt
     4321 aggtgtcggt ggggcgcacg gtggaatcga gcgccaggct cacgaacacg gtggtgccgg
     4381 tgcggttgag cggatggctg cggaactgca ccgactcgcc gcggtggcgc cattgcagca
     4441 cctggccgtt ggactgcagg ttcatcgcgt cgaccatgcg cgcgacgaag aacaggccgc
     4501 ggccgccgtg gcattcgggc tgcgtcgtca ggcggccctt ggagatctcg agcgcggcgc
     4561 gctgggcgtc gggcagctcg gccttggcgg caagctggtt gaacacgccc aggccgtcgt
     4621 cgccgatcag gatgtgcagg tgggtcggcg tctggcgcac cgacacggtc acgcgcgtgg
     4681 cctgcgcatg gtcgatcgcg ttgttcagca gctcgccgaa aacgtgctgc gcgacgcgcg
     4741 cgacggccgg gctcagctgc agcacggggt ggacgtcgcg cagccacggc gtgtgctcgt
     4801 ccaggccggc gatcgcgtac tcattggtga cctcgcgctg cacgcccggc ttgaagtgcg
     4861 gacgcgtgac gcccgtgcgt acgagccagc cgctgtcgac caggcgcttg agctgcttgg
     4921 tcatcgtgcc gcgcgagacc gagtggcgcc tggccagcgt cgggccgagg tcggtcgggt
     4981 gcgccagggc gtcggcggtg atcgtgcggg tcagggtagc gagttcgatg cgggccatca
     5041 ggttctccgg gccgtccccg gacgggcggc gcactcggca gagtttcccg gacctgtcac
     5101 tcggggagcc gccgaacaag gccggaaaac cggcggagtt cggcgctccg gcggggggca
     5161 ggtcttgaat cgcgcggatg gggtcaggtc ttgaaagttg cgtgctcgac ttgcgagcgt
     5221 cggccatggc gccgccacgc aactttcaag acctgacccc atgcttgcat gaccccatcc
     5281 ttgcaccgcc gcccacgcaa cgattcaaga cctgacaccg gactggcgcc ggcgtgggtg
     5341 gcccgctcag gtcggcctgg cgacgccggc agcctcgaag agggcgtcct cgagcgcccg
     5401 caccggcgcc tggtcgaaga acagtgcgtg gcggcgctgg gcgatctcgc gctccaaatg
     5461 ggcgcgccag gcctcgtcct ccgccagccg caatgcgatc gcggcgaacg cctccggggt
     5521 gcccgcgacc agctcgtcca gcccgaggtg gcgcagcggg ccgctggcca ggcggccgcg
     5581 catgaagggg ccctcgaacg cgacgaccgg caggtcgcac tcgagcgcct gcacggcggt
     5641 gttgaagccg gaaaagccga gcgtgtccag cagcgccgtg gagcgccgca tcaggccgtg
     5701 gaagcggccg cgggtgagcg tgggcaccca ggccacggtg cggtcgtagt cgacgccggc
     5761 cttgccgaat gcctggcgca tgcgctgctc gacctggcgc gacatccccg ggcgatggct
     5821 caggaagaac acgaggcggc cacggccgcg cgcctgcagg cccttggcga tcgccacgag
     5881 cgcggcgtcg ccctgcggcg tgtacttgaa cggcgtgccg gggcacagca gcagcggcgc
     5941 gtcggggttc aggcccagcc cggcgaagtc gggcaccacc tcttcgggct gcagcggctg
     6001 caggcacacg cccaggtgcg gcagcgcgac cagcttttcc gcgtagtggc tcgccgcgcc
     6061 gtcgggctcg agcgcctggg cggacaggaa caggtccatc gtgggcagcg cgctggtcag
     6121 cggctggccc caggccacgg cctgcacagg cgccagccgc agcgcggcca ggcgcgccgt
     6181 gagcgggtgc atgccgatct cggggtagat cagcacgtcg aggcgggcgt cggtgatcgc
     6241 gcgggtccac tcgtcgagcg tcgtcggcgt gtcgacgaac gcggccgcgt cgcggcgcgc
     6301 gcgggccgtc tcgtcgtcgc tggcgcgatc gagcttgaag atcgtcagct cgatgcgggc
     6361 gcggtccagg tgccggaccc agccctgcgt gatcgcgttc cacacggagt ggtcgcgcac
     6421 gtgggcggcg gcgatgccca cacgcagctt gcgcgccgcg cccgcgctcg cgcgtgacgt
     6481 ctgcgcaccc gcgagcggcg cgccggcggc catcaggcgc gtggcgagct tgccgtacgg
     6541 cagcagcagc gctcgcacgt cgacggggtg gtacgcgagg tagaacggca gcgcgctgcc
     6601 cacggccgtc gaaccggcgg cggcgcgagc gggcgtgaac cacgcgtcga gctcgccgag
     6661 cgcgcgcgtg aaggccttgc gcgatgcgtc catctgggcg gcgtcgtcgt agaccggccg
     6721 cagatgcgac atcgccagcg cgaagcgggc gcgcgcgttc tgcggatcga gttcgacggc
     6781 gcgcgcgaac tgcctggtcg cggcggcggc atcgcccgcg ccgaacagcg cgttgccatg
     6841 gccgaggaac gtggccgcgt ccgcccccgg cgcccccacg gcgcgggcgt agagctcgac
     6901 cgcgtcctcc atgtggccga gcgcgaactg cgccgacgcc tgcatcgcga gcgccgcgct
     6961 gctgccgggg tgcgactggc cgaaggcctg gccgtgccgc agcgcttcgt cgccgcggcc
     7021 gccgtccacc agcgcgccga ccagcgcctc gtgcgccgcg gccagctgcg ggtcgagcct
     7081 gagcgcctcg cgcaggtgcg tgagcgcggc gtcgatctcg ccctgctcgc gcaggaacac
     7141 gccgaagttg tggtgcgcca cggcatcgcg cggcgcgccg cgcacgccgg cgtcgaaggc
     7201 ggtgcgcgcg gcggcgcggt cgccgagcgc ggccagcgcg cggccacgcg cgccgtgcgc
     7261 cggcgcggcg ggctcgaggg ccagcgactg gtcggccgcc tccagcgcgc gcttggggtt
     7321 gccggccagg tcgcaggcga tcgacaggtt cagccaggcc cgggcgtcgc gcgggcgcga
     7381 cttcacgctg gtctcgaaga tggcgacggc gccgcggccg tcgcccgtct ccagcagcga
     7441 ggcgcccagc aggtcggcga tctcggccga tttcgggctg gcgccgtacg cgcgcttgag
     7501 cggcaccagc gattcggcga cgcgtccgag ctggcgcagg acgttgccca ggttggactg
     7561 cgcgtccgcg aacgtgggct gcgccttggc ggcgcgctcg aactgctcgc gcgcctcgtc
     7621 gagccgccgc gcgcgcagca gcacggtgcc caggttgttc agcgccgccg cgtccttggg
     7681 cttgctcgcg agcgcctggg tgatctgcgt ctccgccgtc gcgaggtcgc cgcgctgcag
     7741 cgcgatcacg cccagcaggt gcgtggcgtc gaactggccg gggaactgct gcaggaactg
     7801 ccggtaggcc gcggcggcct cttccagcct gccggcctgg tgcagcgcca cggcctggtt
     7861 caactgggcc gtcgcgggcg agacggccgt gtacgaggac gggcgcggca tcagacctcg
     7921 cgattggcgt tggccgccgc cacgacgctg gccagcgcat aggcgaattc gtcgacgaag
     7981 ctcttggggt acctggcttc cagcagcgcc cacttcatgc ggttggcgat gcgcgccttc
     8041 tggtacaggt tgggcttctc gcgctggacg aagtccttgg cgtggcgcag gagcaggtcg
     8101 tgccccttgg ccagctgttc ggccgccttc ctggcgccgg cgccgtcgcc ctcgagcctg
     8161 gccggcggga cacgcttgac gaggtccgcg acctcggcgt cgacgaacgc atcgaccttg
     8221 cttgtgttga accaggaaaa gaccatcgga aacccgtcgt tgcgattgcg gaccaggggc
     8281 gattatgcgg tccagtaccc gaccttctag aatgggcaag ttacggcgca gttacgccac
     8341 cgcgccgccg gatttcgtgg caccgtcggg ttcgtgagcc ggagacagct cacggcaccg
     8401 gttgccgaaa cgcatttgca acatccttca aaggacttcc catgcccgtc gtttccatgc
     8461 gccaactgct ggaccacgca gccgagaacg gctacgggat cccggccttc aacgtcaaca
     8521 acctggagca ggtgcaggcc gtgatgtcgg ccgccgccga agtcggcgcc cccgtgatcc
     8581 tgcaggcatc cgccggcgcc cgcaagtacg ccggcgaacc gttcatcaag cacctgatcc
     8641 aggcggccac cgagagctgg ccgcagatcc cgctggtgat gcaccaggac cacggccaga
     8701 accccgaagt ctgccgcggc gcgatggacc tgggcttcag ctccgtcatg atggacggct
     8761 cgctgcaggc cgacggcaag accatcgcca gctacgacta caacgtggac gtcacccgca
     8821 ccgtcgtcga catggcgcat gcgcgcggca tcacggtcga aggcgaactg ggctgcctcg
     8881 gctcgctcga gacgatgaag ggcgacaagg aggacggcca cggcaccgac gcgacgatga
     8941 cgcgcgacca gctgctcacc gatcccgagc aggccgccga cttcgtcaag cgcacgcagc
     9001 tggacgcgct ggccatcgcc atcggcacca gccacggcgc ctacaagttc acgcgcaagc
     9061 ccacgggcga catcctggcc atcgaccgca tcaaggagat ccaccgccgc atccccacca
     9121 cccacctggt gatgcacggc tcgtcctcgg tgccgcagga cctgctggcg atcatccgcc
     9181 agttcggcgg caacatgaag gagacctacg gcgtgccggt cgaggagatc caggaagcca
     9241 tcaagcacgg cgtgcgcaag atcaacatcg acaccgacat ccgcctggcg atgaccggcg
     9301 cgatccgcaa gtacctgttc gagaacccgg acaagttcga cccgcgcgac tacctcaagc
     9361 ccgcgcgcga ggccgccaag gcgatctgcc gccagcgcta cgtcgagttc ggctgcgaag
     9421 gccaggccgg caagatcaag acggacacgc tgcaggtggt ggccgccaag tacgccagcg
     9481 gcgagctcgc gcagacggtc cattgagccc ccacgccccg gagggggcgc tgcgcagcgg
     9541 tcgctgtccc gcgaggggag gcttcgcgcc cgcgccctcc aacttctcac ggaagcgacc
     9601 tgcgacggcg tcgggcaatg aagaactgca gcgctcaggc aggcacaata cgggtttagc
     9661 cgaccaaagc ctgcccgatc catgaccgcc gcgctgctca ccacctccat ccactccctg
     9721 ccgctgctgg cgcgcggcaa ggtgcgtgac aactatgccg tcggcgacga ccgcatcctg
     9781 atggtggcca ccgaccgcct gtcggcgttc gacgtcatcc tgggcgagcc gatccccggc
     9841 aagggcgccc tgctgacgca gatggcgctg ttctggttcg gcaagctcga gggcatcgtc
     9901 ccgaaccacc tgaccggcga ggcgccggag tccgtcgtgc aggccgacga ggtggcgcag
     9961 gtgcgcgagc gctcgatgct ggtcaagcgc ctgaagccgc tgcccatcga ggcggtggtg
    10021 cgcggctacc tggcaggctc cggctggaag gaataccagg cgagccagtc ggtgtgcggc
    10081 gtgcagctgc ccgccggcct gaagaacgcg tcgaagctgc ccgagcccat cttcacgccc
    10141 gcgaccaagg ccgaggccgg cgagcacgac gagaacatca cgttcaaggt ggccagcgcc
    10201 atgatcggca acgagctggc caaccacgtg cgcgacgtct ccatcgcgct gtacgagcgg
    10261 gccgccgaat acgcgctgac caagggcatc atcatcgccg acacgaagtt cgagttcggc
    10321 ctcgacg
//
LOCUS       DATCRO010000301         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_295752, whole genome shotgun sequence.
ACCESSION   DATCRO010000301 DATCRO010000000
VERSION     DATCRO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295752"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIN75_08145"
     CDS             complement(<1..305)
                     /locus_tag="VIN75_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY8974174.1"
                     /translation="MSLVDRWICGAEVALRTLSGGSRAERATPLPAPAGDPPDAVVPA
                     ELAPADKRLSGALMRVNHVGEVCAQALYQAQAVATSDPALKARMAQAAREEVDHLAWT
                     "
     gene            547..1620
                     /locus_tag="VIN75_08150"
     CDS             547..1620
                     /locus_tag="VIN75_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8974175.1"
                     /translation="MKKSLLALAVLTAVSGAAFAQSSVTLYGKVDLGLVLDSGSAAGK
                     SVRLDSGVTGGSRIGFKGVEDLGGGMKASFQLETGYCADSAAPATSTLTVKDGAGNTV
                     GTTTQTSTNFCTGSNAFMGRQAHGDLTGAFGAISAGRQYSIGFNNLSTIDPFGTGFAG
                     QINNIVDPSGIRLNNSVTYATPNMGGFVGSAEIAFGEQTGNWEANRETGAGLTYASGP
                     AYVGFSFYDVSNSAGNGAARKNYTLGGTYDFGVIKLHALAQKSTGPSTLDVIDLMGGV
                     TVPVAGGNMMASYIHHNDRTVADKDANQIGIGYIYPLSKRTSVYSSFARIQNQHGAAF
                     TVGNATSSGTGDKAFNVGVVHNF"
     gene            1799..2788
                     /locus_tag="VIN75_08155"
     CDS             1799..2788
                     /locus_tag="VIN75_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974176.1"
                     /translation="MLPFILRRLAQAVLVMLVVGLGAFSLFQYVGDPVSNMVGPDPSA
                     AQRERLHAELGLDEPAPVQFARFVRHALAGDFGLSLRQGREVSALIAERLPATLELAV
                     VAALISVLVAVPLGVHAALRRRHWDARLTMAVSLLGVSLPTFLVGIVLIEVFSVLLGW
                     LPSFGRGATVQVGGWSSGLLTLDGWRHILLPAVTLAVYQLALLMRLVRGEMLEALRTD
                     YVKFARARGLPDRVVHFGHALRNALMPVLTISGMQLGSLIAFSVITETVFAWPGMGSL
                     FIQAVQFPDVPVMAAALCLIALMFVLINLVVDLLYVVVDPRLRLHGARAGASR"
     gene            2785..3747
                     /locus_tag="VIN75_08160"
     CDS             2785..3747
                     /locus_tag="VIN75_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974177.1"
                     /translation="MSARLPSTPDAGRAATTRHARSADRSGGGLRHDFVRSPTALLAA
                     AVALLCLAGALLAPWLAPHDPSDLAHLDLADSHLPPAWLADGRATYPLGTDDQGRDML
                     SALLYGLRISLMVGLSSVALSVAIGVTTGLLAGFAGGFVDAALMRLSDVMLSFPPILI
                     ALLIDGVGRVVFPHAHEVLAFAVLIAALTLTEWVRYARTVRGSALVERHKEYVQAARL
                     AGVPTLRLLRRHVLPNVLGPVLVLATIQVGQAIVAEATLSFLGVGVPPTTPSLGTLIR
                     IGNDFLYAGEWWITLFPAAALVLVALSVNLLGDWLRDALNPRLQ"
     gene            3744..>4218
                     /locus_tag="VIN75_08165"
     CDS             3744..>4218
                     /locus_tag="VIN75_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974178.1"
                     /translation="MTGPLLEVRDLCVEFPTRRGTPRALDGVTFDIAAGEILGVVGES
                     GAGKSLTGAAIIGLLEPPGRMAAGQVLLESQRIDDLPREAMRLVRGRRIGAIFQDPLT
                     SLNPLHTIGHQLVETLRTHLPLGAAQARERAIGLLRQVGLPAPQARLAQYPHEFSG"
BASE COUNT          546 a         1514 c         1473 g          685 t
ORIGIN      
        1 gtccaggcga ggtggtcgac ctcctcgcgc gcggcctgcg ccatccgggc cttgagcgcc
       61 gggtccgacg tggccacggc ctgcgcctgg tagagagcct gggcgcagac ctcccccaca
      121 tggttcacgc gcatcagcgc gcccgacagg cgcttgtccg cgggcgccag ctcggccggc
      181 acgaccgcgt ccggcgggtc gccggcgggc gcgggcagcg gcgtcgcgcg ttcggcgcgg
      241 ctgccgccgg acagcgtgcg cagcgccact tcggcgccgc agatccagcg atcgacgaga
      301 ctcatttcca tgccggaacg atacccgatc gggccggaaa ggccccgcct gccaccaaat
      361 ggggccactc aatgtgacgt tgtgcgagga caacaggcgc ccgcgatttc ggccgccatt
      421 cctttgcaac cgtgggcccc gatggtgcaa tacgtccagc ttccgatgag gttgcggtcc
      481 ttgcggtgaa acacgcccag ccgcgccgcg accggaatct cttgttaacc ctaggagata
      541 ttcgcaatga aaaagtcgct gctcgctctc gcagtgctca ccgccgtgtc cggcgccgct
      601 ttcgcccaat cgtccgtgac cctgtacggc aaggttgacc tgggcctggt tctcgacagc
      661 ggcagcgctg ccggcaagtc ggtccgcctg gactccggcg tgactggcgg ctcgcgcatc
      721 ggcttcaagg gcgtcgaaga cctcggcggc ggcatgaagg cctccttcca gctggaaacc
      781 ggctactgcg ccgacagcgc cgcgccggct accagcaccc tgaccgtcaa ggacggcgcc
      841 gggaacacgg tcggcacgac cacgcagacc tccacgaact tctgcacggg cagcaacgcc
      901 ttcatgggcc gccaggccca cggtgacctg acgggcgcct tcggtgccat cagcgccggc
      961 cgccagtaca gcatcggctt caacaacctg agcacgatcg acccgttcgg cacgggcttc
     1021 gccggccaga tcaacaacat cgtcgatccg tcgggcatcc gcctgaacaa ctcggtgacg
     1081 tacgccaccc cgaacatggg cggcttcgtc ggttctgctg aaatcgcctt cggcgagcag
     1141 accggcaact gggaagccaa tcgcgaaacc ggcgccggcc tgacctacgc ctcgggcccg
     1201 gcctacgtcg gcttctcgtt ctacgacgtg agcaacagcg ccggcaacgg cgccgcgcgc
     1261 aagaactaca cgctcggcgg cacctacgac ttcggcgtca tcaagctcca cgccctggcc
     1321 cagaagtcca ccggcccgtc gaccctggac gtcatcgacc tgatgggcgg cgtgaccgtt
     1381 cccgtcgccg gcggcaacat gatggcttcg tacatccatc acaacgaccg cacggtcgcc
     1441 gacaaggacg ccaaccagat cggcatcggc tacatctacc cgctgtccaa gcgcacctcg
     1501 gtgtacagct ccttcgcccg catccagaac cagcacggcg cggccttcac ggtcggcaac
     1561 gccacgtcgt ccggcacggg cgacaaggcg ttcaacgtgg gcgtcgtcca caacttctga
     1621 tcgccgcccg gcctggccgg gatgctcagt gctccgcctc gcgcggagcc ccttcgaggc
     1681 tcgtctgcgg acgggccttt ttcattgcgc ggcgtcggca gcagccaggc ccgttcgctg
     1741 cccactgcgg tggaacccag ggcgcgccgg cggcccttcg ggggtattct tgccctcgat
     1801 gctgccgttc atcctccgcc gactcgcgca ggccgtgctc gtgatgctgg tcgtcgggct
     1861 gggggcgttc tcgctgttcc agtatgtcgg cgatccggtc agcaacatgg tcgggccgga
     1921 tccgagcgcc gcgcagcggg agcggctgca cgccgagctg ggcctggacg aaccggcgcc
     1981 cgtgcagttc gcgcgcttcg tccgccatgc gctggccggt gacttcggcc tgagcctgcg
     2041 ccagggccgc gaggtctcgg cgctgatcgc cgagcgcctg ccggcgacgc tggaactggc
     2101 ggtggtggcg gcgctgatct cggtgctggt cgccgtgcca ttgggcgtgc acgccgcgct
     2161 gcggcgccgt cattgggacg cgcggctgac gatggcggtg tcgctgctgg gcgtgtcgct
     2221 gcccacgttc ctggtgggca tcgtgctgat cgaggtgttc tcggtgctgc tcggatggct
     2281 gcccagcttc ggccgcggcg ccacggtgca ggtaggcggc tggagctcgg ggctgctgac
     2341 gctcgacggc tggcgccaca tcctgctgcc ggcagtcacg ctggccgtct accagctcgc
     2401 gctgctcatg cggctcgtgc gcggggagat gctggaggcg ctgcgcacgg actacgtgaa
     2461 attcgcacgc gcccgcgggc tgccggaccg cgtcgtgcac ttcggccacg cgctgcgcaa
     2521 tgcgctgatg cccgtgctca cgatctcggg catgcagctg ggcagcctga tcgcgttctc
     2581 ggtcatcacc gagaccgtgt tcgcctggcc cggcatgggc agcctgttca tccaggccgt
     2641 gcagttcccc gacgtgccgg tgatggcggc cgcgctgtgc ctcatcgcgc tgatgttcgt
     2701 cctgatcaac ctggtcgtcg acctgctgta cgtggtcgtg gatccgcggc tgcgcctgca
     2761 cggcgcgcgc gccggggcca gccgatgagc gcgcggctgc cgtcgacgcc ggatgcgggc
     2821 cgcgcggcga cgacgcgcca tgcgcgctcg gccgatcgca gcggcggcgg tctccggcac
     2881 gacttcgtgc gttcgccgac ggcgctgctg gccgccgcgg tggcgctgct gtgcctggcc
     2941 ggcgcgctgc tcgcgccgtg gctggcgccg cacgatccgt ccgacctggc gcatctggac
     3001 ctggccgact cgcacctgcc gccggcctgg ctggccgacg gacgggcgac ctatccgctg
     3061 ggcaccgacg accagggccg cgacatgctg tcggccctgc tgtacggcct gcgcatctcg
     3121 ctgatggtgg gcctgagctc ggtggcgctg tcggtcgcga tcggcgtgac gacgggcctg
     3181 ctcgcgggct tcgcgggtgg cttcgtcgat gcggcgctga tgcggctgtc cgacgtcatg
     3241 ctgtcgttcc cgccgatcct gatcgcgctg ctgatcgacg gcgtgggccg cgtcgtcttc
     3301 ccgcatgcgc acgaggtgct ggcgttcgcc gtgctgatcg ccgcgctgac gctgaccgag
     3361 tgggtgcgct acgcacgcac ggtgcgcggt tcggcgctcg tggagcggca caaggagtac
     3421 gtgcaggccg cgcgcctggc cggcgtgccg acgctgcggc tgctgcgccg ccacgtgctg
     3481 cccaacgtgc tcgggccggt gctggtgctg gccacgatcc aggtcgggca ggcgatcgtg
     3541 gccgaggcga ccctgtcgtt cctgggcgtc ggcgtgcccc ccacgacgcc gtcgctgggc
     3601 acgctgatcc gcatcggcaa cgacttcctc tacgccggcg aatggtggat cacgctgttc
     3661 ccggccgcgg cgctggtgct ggtcgcgctg agcgtgaacc tgctgggcga ctggctccgc
     3721 gacgcgctga acccgaggtt gcagtgaccg gtccgctgct cgaggtgcgc gacctgtgcg
     3781 tcgagttccc gacgcgccgc ggcacgccgc gcgcgctcga cggtgtgacg ttcgacatcg
     3841 ccgccggcga gatccttggc gtcgtcggcg aatcgggcgc gggcaagtcg ctgaccggcg
     3901 ccgcgatcat cggcctgctg gaaccgccgg ggcgcatggc ggccgggcag gtgctgctgg
     3961 agagccagcg catcgacgac ctgccgcgcg aggcgatgcg cctcgtgcgt ggccggcgca
     4021 tcggcgcgat cttccaggat ccgctgacct cgctgaaccc gctgcacacg atcggccacc
     4081 agctcgtcga gacgctgcgc acgcacctgc cgctgggcgc cgcgcaggcg cgcgagcggg
     4141 cgatcgggct gctgcgccag gtcggactgc ccgcgccgca ggcccgcctg gcgcagtacc
     4201 cgcacgagtt ctcgggtg
//
LOCUS       DATCRO010000302        13363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_756717, whole genome shotgun sequence.
ACCESSION   DATCRO010000302 DATCRO010000000
VERSION     DATCRO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756717"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VIN75_08170"
     CDS             complement(<1..377)
                     /locus_tag="VIN75_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8974179.1"
                     /translation="MSHDPTSYPDLTRAISRRVSSLRDGQPEAMHGFRQLSDGAMAAG
                     AIDAKTKELMALALGIAAHCAACIGFHAKALVALGARRAEIEETVAVAVYMGGGPSLM
                     YGADALAAFEQFGEKAAAHAAKT"
     gene            complement(439..1362)
                     /locus_tag="VIN75_08175"
     CDS             complement(439..1362)
                     /locus_tag="VIN75_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY8974180.1"
                     /translation="MHCAYITHADCMRHEMGPSHPESPARLSAIHDRLLFDGILDFMS
                     VHDAPMATQEQLCRVHTPEHVAHVMASTPMDGYVQVDPDTSMNPHTVVAALRAAGAVV
                     LATDLVLGERADAAFCCVRPPGHHAERAAAMGFCFFDNVAVGIRHALDVHGLDRVALV
                     DFDVHHGNGSQDIFRDDPRVLMCSTYELDLYPFPTDTTGPNMVNVGLPPRSGGEAFRR
                     AVEEIWIPAIDLFQPQLIVVSAGFDGHRADDMGNLGLVEADYQWVTCELVKLARRHCG
                     GRVVSSLEGGYHLPSLARSVAAHVSALIGAA"
     gene            complement(1376..1831)
                     /locus_tag="VIN75_08180"
     CDS             complement(1376..1831)
                     /locus_tag="VIN75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8974181.1"
                     /translation="MYSRILVPVDGSPPSLAGLKEAITLATDGGATIVVLNVASELPL
                     ATAPEGFVAYREFVDALSAAGRAIVKEAGKEVEKAGVKVESLVLDVGMSPVSDAIVEQ
                     IGRQRCDLVVMGTHGRRGLKRLTLGSDAELVVRQSPVPVLLVKAPPAAK"
     gene            complement(1982..2809)
                     /locus_tag="VIN75_08185"
     CDS             complement(1982..2809)
                     /locus_tag="VIN75_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8974182.1"
                     /translation="MGALRRVVLALLACAWLACAGAWGQAAVPPATAHVIDQTGSLDA
                     AQSGTLETRLAAFEREHGAQVVVLIVPTTAPETIEQFGIRVADQWRIGRKGVDDGVIV
                     LVALQDRAARIEVGYGLEGVLTDVASHRILAETMEPKLRAGDVAGGVSDGVDRILSML
                     SSVPAPASGTSAGQASPRDDAGEYSPYFWVVVVAIALGGLLRRAFGPVKAAALTAPVG
                     ALLGWLAGGSIVVILAAAALAAVLAALGISAWLPWGGGSSFKGRGGGFGGGGASGRW"
     gene            complement(2811..3464)
                     /locus_tag="VIN75_08190"
     CDS             complement(2811..3464)
                     /locus_tag="VIN75_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8974183.1"
                     /translation="MNPRSIIGTALRGLGALVLALLLGNLAGCGYNRLVSADEDVKAC
                     WSDVLNEYQRRADLIPRLVDVVKGEAAFERSTLTQVIEARARATSVQATPALVNDPEA
                     FARFEQAQLQLGGALSRLLAVAESYPTLQASQAFRDLQSQVEGTENRIAVARHRYIQA
                     VNAYNTQVRSLPTNMTAWAFGFQAKANFRGEDEARIAQPPRIDFGNPAPASAASAGG"
     gene            3642..4100
                     /locus_tag="VIN75_08195"
     CDS             3642..4100
                     /locus_tag="VIN75_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8974184.1"
                     /translation="MYRRILVPIDGSPTARSGLDEAIRLARQTGASLRVFHAIDDPFT
                     ALGIDEVVSAPRNIDALLEQNARRIVDDAMAAARLAGVYADSEIEDRLHGPLQDLVAE
                     AALTWKADLIVMGTHGHRGMVRMLLGSDAEQILRAAPVPVMLVHGPREEQ"
     gene            4142..5959
                     /locus_tag="VIN75_08200"
     CDS             4142..5959
                     /locus_tag="VIN75_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8974185.1"
                     /translation="MAPATMPPSSVRTLPDLLAWRAKTTPWRPAYSEFDAGRDAWREV
                     TWRGTGDAVRRISHLLASLRLEHGARVALLLPNGHRAACLDLAVLASGLVPVPLHALD
                     NPASIAYILQDTAAQVLVVDSAAQWRAIRALGAPPGSLRLVLVRRDPEGVVGKDGAAS
                     VLSLDDWLNGPGVALGLPLHAPGEDDLAAIVYTSGTTGRPKGVMLTHANVMSNVRACL
                     ARFEAREDDVFLSFLPLSHTFERTAGFYLPIAAGSCVAYARSVQSLPDDLLRTRPTIL
                     VSVPRIYEKVQAQIETTIAGSWLARVAFRCAVAAGWRRHLRDMELSAAAEWLRPLDAL
                     LTRLLDARVGAPLRARFGGRLRVAVSGGAALVPDIARTFIGLGIEIVQGYGMTESSPV
                     VTANAPGDNDPATVGRPLDGVEVRIGPSLELQVRGPNVMRGYWNRPEDTAAAFCDGWL
                     RTGDQAAIEDGRVRILGRIKEIIVTATGEKIAPVDVEQALMRDPLIEQAYVFGEGRPF
                     IGCVLQLQAREWAFLCGGLGLDAEAPESLFAPEAVRAVEARMTARTATLPRHGQPRRA
                     ILTLAPWTTDNGLLTPTLKLKRRNLQARFASEIEQLYEG"
     gene            complement(6109..8784)
                     /locus_tag="VIN75_08205"
     CDS             complement(6109..8784)
                     /locus_tag="VIN75_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY8974186.1"
                     /translation="MSVRNLDALLAPRSVVVVGASDRPATVGGTVWRNVRAGGFAGAV
                     HAVNPAHRELDGVAVFGRCSDLPEPADLAVVCVPPAAVAPTIAELAQAGTRAAIVVTA
                     GLSPTDKAAMLEAARPHLMRVLGPNCVGLLSPHQRLNASFAHTDAPAGDLAFVTQSGA
                     LLTAVLDWAKARGVGFSSLVSLGDHADVDFGDLLDHLGSDVHTRAILLYIESIESPRK
                     FMSAARAAARNKPVVVVKAGRAGGGVRAAASHTGALAGSDIVFDAALRRAGVLRVDTL
                     EELFLGAETLTRLRGPIEGGLTLFTNGGGAGVVAADAAARAGIALPEPDPATLAALSA
                     CLPPTWSHGNPVDIIGDAPAQRYVDALRVLLDAPSTGTLLFMHAPTAIVDSRDIARAC
                     LPLLQPRRERVLACWVGATAVADARRQFEEAGIACLDTPEEAVRAFEMLSDYRRNQAL
                     LLEAPSAADVRPPRVDEARAIVDAAIAGGRTSLDEVDAKRLLGAYGIPIVATRRTALS
                     AEAAVDAARAIGWPVALKILSPDLSHKSEAGGVALDLVDETAVRQAADVMLATVRERR
                     PQARLEGFSVQAMARRPHARELIVGASLDATFGPVLMVGQGGTAVEMLGDRAIALPPL
                     NRVLARDAIGRTRVSRLLQGWRDHPAARLDAVADALIALARMQADLPELAELDINPLW
                     ADEDGVLALDARLRLQADRPAGVARFAILPYPAELASHVEWDGRDLVLRPIRPEDEPM
                     HAAFLDRCSPDDLRLRFFSVRRSLPRSEIARLVQIDYAREMAFVAVAPGDDGQDEILG
                     VSRAVSDPDGVEAEFAVLVRSDLHKRGLGHLLMARIVDWATKRGTQRLFGDVLHDNEA
                     MRILMRECGFAATPSPEHGATRFVLELAGAVAQNL"
     gene            8998..9492
                     /locus_tag="VIN75_08210"
     CDS             8998..9492
                     /locus_tag="VIN75_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8974187.1"
                     /translation="MYAKILVPVDDSRAAEAALDEAIRLARLCGGELRLLHVSPDPPF
                     VPASSVFRDEAQAEASEGARRGSALLARLGEHVGAQGIGVDTVLLETRGCHLHGLVNE
                     QARAWGAELVVLGTHGRRGVERALVGSVAEQVIRHAEVPVLLVRRPEAPQDTGLQSIA
                     AAIA"
     gene            complement(9558..10406)
                     /locus_tag="VIN75_08215"
     CDS             complement(9558..10406)
                     /locus_tag="VIN75_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8974188.1"
                     /translation="MIYPTLLLHLDDDKRADARNAQAVAFALKIGAHVNGLSCHRPAA
                     MGADFAAAMGGPTDPLTRELQRAQQQAVERERRFRSLCERLALPSFESTIDDTEEAGR
                     AILRRAALHDLVVLGQPDPDDAQAAGRRDFVADVVQNSPRPTLLFPYAGNFEEVGRTV
                     LIAWDGSHGCARAASDALPLLERARDVVVVQFDARASVSGPIDQAPLFGIVRWLARHK
                     VEARASVRFTDGDIGNSLLSYAADVGADLLVMGCWGHARWAERLLGGATRTVMESMTV
                     PVLTSH"
     gene            10723..11481
                     /locus_tag="VIN75_08220"
     CDS             10723..11481
                     /locus_tag="VIN75_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974189.1"
                     /translation="MPDATAAIPLRPCMRILIVDDHAVVREGLKRMLEASEPSWAVEE
                     AANAFEALDRIRAQAFDVAIVDISMPGMSGLDFLKRVRASQMTLRVLMLSMYAEEQYA
                     MRAFKGGANGYVTKDSATRELVSAIRKVTSGSAYVSESLAQSLALGLGNAAGDAAHER
                     LSDRELDILRRLATGDRPTEIAQALHISVKTVSTHKARILARLQLPNSAALVRYAMEH
                     QLIPEGSMRPREDFGGSGWQPLSEDDPVDPEAGA"
     gene            11565..>13363
                     /locus_tag="VIN75_08225"
     CDS             11565..>13363
                     /locus_tag="VIN75_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8974190.1"
                     /translation="MLLPAALALAWRLGDELRESREEALQTVSLLRDDSTRHLSWVLQ
                     HASSLLGFVAAHARMRDGDAAACQATVQAVPLLQPGFMRLEMRDASGRLVCWSSRQAP
                     PTLPPDMDRGPVTGLAVGRVRVDPPTGRLVVPLSQAVVDDAGVVQGRLRLLLDMGSMS
                     EELSRNAAAGAGAVITVIDPDATILMRTHHAEEFIGKRGSIPDPSGGQGTGFIDGAGR
                     DGIDRLYAYGTVPEAGWRVFAGLPRDHVYAGYQETRRRTIAVGSMVLALACVMAWQLA
                     SVIARPMASLQAAARRVGAGDLGHVTVEGPPELRDVAEDFNHMVDALSLSRSRLQALF
                     DTMSEAVITVDDAQTVVMANPAAATLLRCAMPVLIGSKLDRWLPERVREAHRLDVERF
                     GTSASGPRDMGHRPEISALRHDGEETPIEASISLVQVEGRRFYTAVLRDVSERRRTMN
                     ALARNKALLTAALANMSDAVAILDAQGRFIAVNDAFTTFYRLPPGSPPLANVQELAAL
                     LDIRLPDGRAAGPHECAGLRAIAGESGTGVLYKLRRLDGGDGWTGSFNFAPIRDAHGA
                     ITGAVSTARDVTAQLAAQHELEHSRDALRRLVGS"
BASE COUNT         1947 a         4834 c         4736 g         1846 t
ORIGIN      
        1 catgtcttgg ccgcgtgcgc agccgccttc tcgccgaact gctcgaaggc cgccagcgcg
       61 tccgcgccat acatcagcga cgggccgccg cccatgtaga ccgccaccgc gacggtctcc
      121 tcgatctcgg cgcgccgggc gcccagggcc accagcgcct tcgcatggaa gccgatgcag
      181 gcagcgcaat gcgcggcgat gcccagggcc agcgccatga gctccttggt cttcgcgtcg
      241 atcgcgcccg cggccatggc gccgtcggac agttgcctga atccgtgcat cgcctcgggc
      301 tggccgtcgc gaagagagga cacccggcgc gagatggctc gtgtcaggtc gggataggag
      361 gtcggatcat ggctcatggg tgcatcttgc ttgcacgggc gaatgccgcg ttgcgcctgg
      421 tcaatcaagc gcccgtcgtc acgcggcgcc gatcagcgcc gacacgtgcg ccgccacgct
      481 gcgcgccagg ctcggcaggt ggtagccgcc ctccagcgac gacaccacgc ggccgccgca
      541 atgccgccgc gcgagcttca cgagttcgca ggtgacccac tggtagtcgg cctcgacgag
      601 gcccaggttg cccatgtcgt ccgcgcgatg gccgtcgaat ccggccgaca cgacgatcag
      661 ctgcggctgg aagaggtcga tcgcggggat ccagatctct tcgaccgcac ggcgaaaggc
      721 ctctccgccg gagcgcggcg gcaggccgac gttgaccatg ttcggcccgg tcgtgtcggt
      781 cgggaacggg tagagatcga gttcgtaggt cgagcacatc agcacgcgcg gatcgtcgcg
      841 gaagatgtcc tggctgccgt tgccgtggtg cacgtcgaaa tcgacgagcg cgacgcggtc
      901 caggccgtgc acgtcgagtg cgtggcggat gccgaccgcg acgttgtcga agaagcagaa
      961 gcccatcgcc gccgcccgct ccgcatgatg gcccggtggc cggacgcagc agaaggcggc
     1021 gtcggcccgc tcgcccagca cgaggtcggt ggcgagcacc acggcgccgg ccgcgcgcaa
     1081 ggcggcgacg acggtgtgcg ggttcatgct ggtgtccggg tcgacctgga cgtagccgtc
     1141 catcggcgtc gaggccatga cgtgcgccac gtgctccggc gtgtggacgc ggcacagttg
     1201 ctcctgtgtc gccatcggtg cgtcgtgcac gctcatgaaa tcgaggatgc cgtcgaacag
     1261 caggcggtca tggatggctg acaggcgcgc cggcgactcg ggatggctgg gccccatctc
     1321 gtggcgcatg cagtcggcat gcgtgatgta tgcgcagtgc atggcgacca ggccgtcact
     1381 tggcggcggg gggcgccttg accagcagca ccggcacggg cgactggcgc accacgagtt
     1441 ccgcgtcgct gcccagcgtc aggcgcttga gcccgcggcg cccgtgcgtt cccatcacga
     1501 ccaggtcgca gcgctggcgg ccgatctgct cgacgatcgc gtcgctgacg ggcgacatgc
     1561 cgacgtccag gaccaggctc tcgaccttca cgcccgcctt ctcgacctcc ttgccggcct
     1621 ccttgacgat cgctcgtccg gcggcggaca gggcgtccac gaattcgcga taggcgacga
     1681 agccctcggg cgcggtcgcg agcggcagct cggaggcgac gttgagaacg acgatcgtcg
     1741 cgccgccatc ggtcgccagg gtgatggcct ccttcaggcc ggcgagcgac ggcgggctgc
     1801 catcgacggg gacgaggatg cgtgaataca tggcagcgga ctccttggcc gtgaccgccg
     1861 atatcgggac tgattcggac ggggttgcat ccattcttcg cgccgcggcg cgaacccacc
     1921 ttgcgctcgc gcaaccgcgg cgacggcgac gtccgcgacc gaccccgtcg ccggcgcggt
     1981 gtcaccagcg acccgacgcg ccgccaccac caaatccgcc gccacgcccc ttgaaggacg
     2041 agccgccgcc ccacggcagc caggccgaga tgccgagtgc ggccagcacg gcggccagcg
     2101 ccgcggccgc gaggatcacg acgatcgacc cgccggccaa ccagccgagg agcgcgccga
     2161 cgggtgccgt cagtgcggcg gccttcaccg gcccgaaggc ccggcgcagc agtccgccga
     2221 gggcgatggc gaccacgacg acccagaagt acggcgagta ctcgcccgca tcgtcacgcg
     2281 gacttgcctg gcccgcactc gtcccggagg ccggcgccgg cacggaactc aacatcgaca
     2341 ggatgcggtc gacaccgtcc gacacgccgc cggcgacgtc gcccgcgcgc agcttcggct
     2401 ccatggtttc cgcgaggatc cggtgcgacg ccacgtcggt caggacgcct tcgagcccgt
     2461 agcccacctc gatgcgggcg gcccggtcct gcagcgcaac gaggacgatg acaccgtcgt
     2521 cgacgccctt gcggccgatg cgccactggt cggccacgcg gatcccgaac tgctcgatgg
     2581 tctcgggcgc cgtggtcggc acgatcagca cgacgacctg ggcgccgtgt tcgcgctcga
     2641 aggccgccag gcgcgtctcc agcgtgcccg attgcgccgc gtcgagggag cccgtctggt
     2701 cgatgacgtg cgccgtcgcc ggcggcacgg ccgcctggcc ccatgcaccg gcgcacgcca
     2761 gccaggcgca ggccagtagc gccagcacga cgcggcgcag cgcgcccatg tcagccgccc
     2821 gcgctcgccg ccgaagccgg ggccgggttg ccgaagtcga tgcgcggcgg ctgggcgatg
     2881 cgggcctcgt cctcgccgcg gaagttggcc ttggcctgga agccgaaggc ccacgccgtc
     2941 atgttggtcg gcaggctgcg cacctgcgtg ttgtaggcgt tgacggcctg gatgtagcga
     3001 tgccgcgcga cggcgatgcg gttctcggtg ccctcgacct gcgactggag gtcgcggaag
     3061 gcctggctcg cctgcagcgt cggatagctc tcggccaccg cgagcaggcg cgacaacgcg
     3121 ccgccgagtt gcaactgcgc ctgctcgaac cgggcgaagg cctcgggatc gttcaccagc
     3181 gcgggcgtcg cctgcaccga ggtcgcccgg gcccgcgcct cgatcacctg ggtgagcgtc
     3241 gaccgctcga aggcggcctc gcccttgacg acgtccacca gccggggaat caggtcggcg
     3301 cgccgctggt actcgttgag cacgtccgac cagcaggcct tgacatcctc gtcggcgctc
     3361 acgaggcggt tgtagccgca gccggccagg ttgccgagca gaagcgccag cacgagcgcg
     3421 ccgaggcccc gcagcgccgt tccgatgatg gatcgagggt tcattcgacc atcgtaggaa
     3481 cgctggcgac gcgcctgttg aggcggatca acccatggcg ccggacggcg tcgatggcca
     3541 cggtcatcga ggccgacctg tcttgcgcga gcgcaagccg cgggttgcac ggtcgccgca
     3601 gactcggcga accaccgcac acggtcatcc cggagctcac catgtaccga cgcatcctcg
     3661 ttcccatcga cggcagcccg accgcgcgca gcggactcga cgaggcgatc cggctggccc
     3721 ggcagaccgg cgccagcctg cgcgtgttcc atgcgatcga cgacccgttc accgccctgg
     3781 gcatcgacga ggtcgtgtcg gcgccccgca acatcgacgc gttgctggag cagaatgcgc
     3841 gccgcatcgt ggacgacgcc atggcggcgg cgcgcctggc cggcgtctac gcggacagcg
     3901 agatcgagga tcgcctgcat ggcccgctgc aggacctggt ggccgaggcg gcgctcacat
     3961 ggaaggccga cctgatcgtc atgggcacgc atggccaccg cggcatggtg cgcatgctgc
     4021 tgggcagcga cgccgagcag atcctccgcg cggcgccggt gcccgtcatg ctggtgcatg
     4081 gccccaggga agaacaatga ggacggaacg cggcgaggcc ggcccgcgaa tcctgcgacc
     4141 gatggccccg gcgacgatgc cgccgtcgtc cgtgcgcacg ctgcccgact tgctcgcctg
     4201 gcgcgcgaag acgacgccat ggcgaccggc ctattcggaa ttcgacgccg ggcgggacgc
     4261 ctggcgcgag gtcacgtggc gggggacagg cgatgccgtg cgccggatct cgcacctgct
     4321 ggcttcgctg cgcctggaac acggcgcgcg cgtggcgctg ctgctgccca atggccaccg
     4381 ggcggcctgc ctggacctgg cggtgctcgc gagcggcctc gtgccggtgc ccctgcacgc
     4441 gctcgacaat ccggcgagca tcgcctacat cctgcaggac acggccgccc aggtcctggt
     4501 cgtcgacagc gccgcgcagt ggcgcgccat ccgggccctc ggggcgccgc caggctcgtt
     4561 gcgcctcgtc ctcgttcgcc gcgaccccga gggcgtcgtc ggcaaggacg gcgccgcgag
     4621 cgtgctcagc ctcgacgact ggctgaatgg gcccggcgtc gcgcttggcc tgcctctgca
     4681 cgcgccgggc gaagacgatc tcgccgccat cgtctacacc tccggcacca cgggccggcc
     4741 caagggcgtg atgctcacgc acgcgaacgt gatgtccaac gtccgggcgt gcctcgcccg
     4801 cttcgaggcg cgggaggacg acgtcttcct gtccttcctg ccgctgtcgc acaccttcga
     4861 gcgcacggcc ggcttctacc tgcccatcgc ggcgggcagc tgcgtggcct acgcgcgatc
     4921 cgtgcagtcg ctgcccgatg acctgctgcg cacgcgcccg accatcctgg tctcggtgcc
     4981 gcgcatctac gagaaggtgc aggcgcagat cgaaacgacg atcgccggct cgtggctcgc
     5041 tcgcgtcgct ttccgctgcg cggtcgcggc cggctggcgg cgacatctgc gcgacatgga
     5101 gttgtctgcc gccgccgagt ggctgagacc gctcgacgcg ctgctcacgc gcctgctcga
     5161 cgcgcgcgtc ggcgccccct tgcgggcccg cttcggcggg cggctgcgcg tcgccgtcag
     5221 cggcggcgcg gcgctcgtcc cggacatcgc gcgcacgttc atcggcctgg gcatcgagat
     5281 cgtgcagggc tatggcatga ccgagtcgtc gccggtggtg accgcgaacg cgccgggcga
     5341 caacgatccg gccaccgtgg gacggccgct cgacggcgtc gaggtgcgaa tcggcccctc
     5401 gctcgagttg caggtgcgcg ggccgaacgt gatgcgcggc tactggaacc gtcccgagga
     5461 caccgccgcc gccttctgcg acggctggct gcgcaccggc gaccaggcgg ccatcgagga
     5521 cggccgcgtg cgcatcctcg ggcgcatcaa ggaaatcatc gtcacggcca ccggcgagaa
     5581 gattgccccg gtcgacgtcg agcaggcgct gatgcgcgat ccgctcatcg agcaggcgta
     5641 cgtcttcggc gagggccggc cgttcatcgg ttgcgtgctg cagctgcagg ccagggaatg
     5701 ggccttcctg tgcggcggcc tcggcctgga cgcggaagca ccggagtcct tgttcgcgcc
     5761 cgaggcggtt cgcgcggtgg aggctcgcat gacggcgcgc accgcaacgc tcccgcgcca
     5821 cggccagccg cggcgcgcga tcctgacgct cgcgccctgg acgaccgaca acggactgct
     5881 cacgccgacc ttgaagctga agcgacgcaa cctgcaggcc cgcttcgcgt ccgagatcga
     5941 gcagctctac gagggctgag aacctgtggc caaatccgcc acacccgctc gtgccgccca
     6001 aagacgccca ctcgtcgttg caaatgctcg ccgtaagcac cgctacgact cgcatttgcg
     6061 cctagattgg acgtctttgg acgacccgct cgggcgcgcc ggattcattc acaggttctg
     6121 agccaccgcg cctgcgagct cgagcacgaa gcgcgtcgcg ccgtgctcgg gcgacggcgt
     6181 ggccgcgaag ccgcactcgc gcatcaagat gcgcatcgcc tcgttgtcgt gcagcacgtc
     6241 gccgaacagg cgctgcgtgc cgcgcttggt cgcccagtcc acgatgcgcg ccatgagcag
     6301 gtggccgagg ccccgcttgt gcaggtcgct gcggacgagg acggcgaact ccgcctcgac
     6361 gccatcgggg tcggacaccg cgcgcgagac gcccaggatc tcgtcctgcc cgtcatcgcc
     6421 gggggcgacg gccacgaacg ccatctcgcg ggcgtagtcg atctgcacca gccgtgcgat
     6481 ctcgctgcgc ggcaggctgc gccgcacgct gaagaagcgc aggcgcaggt catccgggct
     6541 gcagcgatcc aggaacgcgg cgtgcatcgg ctcgtcctcc ggccggatcg ggcggaggac
     6601 caggtcgcgc ccgtcccatt cgacatggga ggcaagttcg gccgggtacg gcaggatcgc
     6661 gaagcgcgcg acgccggccg ggcgatcggc ctgcaggcgc aggcgggcgt ccagcgcgag
     6721 gacgccgtcc tcgtcggccc atagcgggtt gatgtcgagt tccgccagtt ccggcaagtc
     6781 cgcttgcatc cgtgccagtg cgatcagcgc gtcggccacg gcatcgaggc gcgccgccgg
     6841 atggtcgcgc cagccctgca gcagccgcga cacacgcgtc ctgccgatcg cgtcgcgcgc
     6901 cagcacccgg ttgagcggcg gcagcgcgat cgcgcgatcg cccagcatct ccacggcggt
     6961 gccgccctgg ccgaccatca gcacgggccc gaaggtcgcg tcgaggctcg ccccgacgat
     7021 cagctcccgg gcgtgcggcc ggcgcgccat cgcctggacg ctgaagcctt ccagccgcgc
     7081 ctgcggccgg cgctcgcgca ccgtcgccag catcacgtcg gcggcctggc ggacggctgt
     7141 ctcgtccacg agatcgagcg ccacgccccc tgcctccgac ttgtgcgaca ggtcgggcga
     7201 caggatcttg agcgcaacgg gccagccgat cgcgcgggcc gcgtcgacgg ccgcctcggc
     7261 ggacagcgcc gtccgtcgcg tcgcgacgat cggtatgccg tacgcgccca gcagccgctt
     7321 cgcatcgacc tcgtccagcg acgttcgtcc gccagcgatc gccgcgtcga cgatggcccg
     7381 cgcctcgtcc acgcgcggcg gacgcacatc ggccgcgctc ggtgcttcca ggagcagcgc
     7441 ctggttgcgg cggtagtcgg agagcatctc gaaagcgcgc acggcttcct cgggcgtgtc
     7501 caggcacgcg atgccggcct cctcgaactg ccgccgcgcg tcggccaccg cggtcgcgcc
     7561 gacccagcag gccagcacgc gctcgcgccg cggctgcagc aacggcaggc aggcgcgcgc
     7621 gatgtcgcgg ctgtcgacga tggccgtggg cgcgtgcatg aaaaggagcg tcccggtgga
     7681 cggcgcatcc aggagcacac gaagcgcgtc gacatagcgc tgcgccggcg cgtcgccgat
     7741 gatgtcgacc ggattgccgt gggaccacgt cggcggcagg caggcggaca gcgccgccag
     7801 cgtcgccgga tcgggctcgg gcagcgcgat gccggcccgc gccgccgcat ccgcggccac
     7861 caccccggcg ccgccgccgt tcgtgaacag cgtcagcccg ccctcgatcg gaccacgcag
     7921 gcgggtcagc gtctcggcgc ccaggaacag ctcctccagc gtgtccacgc gcagcacccc
     7981 ggcgcggcgc aacgccgcgt cgaagacgat gtcggagccg gccagggcgc cggtgtgcga
     8041 ggctgcggcc cgcacgccgc cccccgcccg ccccgccttg acgaccacga ccggcttgtt
     8101 gcgcgccgcg gcgcgcgcgg cggacatgaa cttgcgcggc gactcgatcg actcgatgta
     8161 gagcaggatg gcacgggtat ggacgtcgct gcccaggtgg tccagcaggt cgccgaagtc
     8221 gacgtcggcg tggtcgccca gcgagaccag cgacgagaag ccgacgccac gggccttggc
     8281 ccaatccagc accgcggtca gcagcgcgcc ggactgcgtc acgaaggcga ggtcgccggc
     8341 cggcgcatcc gtgtgggcga aactggcgtt gagccgctgg tgcggcgaca gcaggcccac
     8401 gcagttcgga ccgagcacgc gcatcaggtg gggccgcgcg gcctccagca tcgccgcctt
     8461 gtcggtcggc gacaggcccg cggtcacgac gatggcggcg cgcgtgccgg cctgcgccag
     8521 ttctgcgatg gtcggcgcga cggcggccgg cgggacgcac acgacggcga ggtcggccgg
     8581 ctccggaaga tcgctgcacc ggccgaagac ggcgacgcca tcgagctcgc ggtgagccgg
     8641 gttgacggcg tggacggcac cggcgaatcc gccggcacgc acgttgcgcc agaccgtgcc
     8701 gccgacggtc gcggggcggt cggacgcccc caccacgacg accgagcgcg gcgcaagcag
     8761 cgcgtcgagg ttgcgcacac tcatcggagt ctcactgtca tcgggcagag cgcgaaggcc
     8821 ggtgcgggcg agttcatgga tggcggacgt gcgggcatgc cgcgacgata ggccgccttg
     8881 ccgttgcgcc gttgcgctgc gtcaagggag ctgccatcgc cggcggttga tgcgcatcaa
     8941 cgagctgcgg cgcccgcgcg ccagactcca tgcatccgaa ccgccgtgga gagcgccatg
     9001 tacgccaaga tcctggtccc cgtcgatgac agccgggccg ccgaagccgc cctcgacgag
     9061 gcgatccgcc tggcccggct gtgcggcggc gagctgcggc tgctgcatgt ctcgccggac
     9121 ccgccgtttg tcccggcgtc ctcggtcttt cgcgacgagg cgcaggccga agcatcggaa
     9181 ggcgccaggc gtggcagcgc gctgctcgcc cgcctcggcg agcatgtcgg agcgcagggc
     9241 atcggcgtcg acaccgtgct gctggagacc cgcggctgcc acctgcacgg gctcgtcaac
     9301 gaacaggcgc gggcctgggg cgccgaactg gtcgtcctgg gaacgcacgg ccgtcgcggc
     9361 gtcgagcgag ccctcgtcgg cagcgtcgcc gaacaggtca tccgccacgc cgaggtgccg
     9421 gtgctgctcg tgcggcggcc cgaagctccg caggacacgg gcctgcagtc catcgcggcg
     9481 gcgatcgcct gaggagaaaa cgcccggcgg gtgccgggcg ctcgcgagcc acggcggcag
     9541 gacgccgcgc gtgtcgctca gtgcgacgtc agcaccggca cggtcatgct ctccatcacc
     9601 gtgcgggtcg cgccacccag caggcgctcg gcccagcggg cgtggcccca gcagcccatc
     9661 accagcaggt cggcgccgac atccgccgcg tacgacaaca gcgaattgcc gatgtcgcca
     9721 tcggtgaacc gaacgctcgc gcgcgcctcg accttgtgtc ttgccagcca acggacgatg
     9781 ccgaagagcg gcgcctggtc gatcggtccg gagacgctgg ctcgggcatc gaattgcacc
     9841 accacgacgt cgcgggcccg ctcgagcaag ggcagcgcgt cggaggccgc gcgggcgcaa
     9901 ccgtggctgc cgtcccacgc gatcagcacc gtccgtccca cttcctcgaa gttgccggcg
     9961 tacgggaaca gcagcgtcgg ccgcgggctg ttctggacga catcggcgac gaagtcgcgc
    10021 ctgccggccg cctgggcatc atccggatcg ggttggccca gcaccacgag gtcgtgcagg
    10081 gccgcccgcc gcaggatcgc gcggccggcc tcctcggtgt catcgatggt gctttcgaag
    10141 gacggcagcg caaggcgctc gcacagcgac cggaagcggc gttcgcgctc cacggcctgc
    10201 tgttgcgccc gttgcagctc gcgcgtcagc ggatccgtcg gcccgcccat ggccgccgcg
    10261 aagtccgctc ccatcgccgc gggtcggtgg cacgacagtc cgttgacatg ggcgccgatc
    10321 ttcagcgcga acgcgacggc ctgcgcgttg cgcgcgtcgg cccgcttgtc gtcgtccagg
    10381 tgcaggagga gggtcggata gatcatgggc aactcccgtt gcgattgaga ccggtgcatt
    10441 ctggaaagcg ccgcgacccg cgggcttgcg cagcctcaag gcggcaacgg ccgccatggc
    10501 ccgtcgaacg acaccgatgg gatccaacgc aatgcccggt gcccacccat gccgtcagac
    10561 gagggttcag ccgcgtcagg aggtcagcca tgctgctcga ggcgcaagcc ttcccgccgc
    10621 ttgcgattcg tcaagcaccg cgcgagcgcg ctcatccaga ctttcgggca atcgaagtcc
    10681 gcaccttcct cgcgtgcgcc ctttggttcc cgaaaggtgt ccatgcccga cgccaccgcc
    10741 gccattcccc tgcgcccgtg catgcgcatc ctgatcgtcg acgaccatgc cgtcgtgcgc
    10801 gaggggctca agcgcatgct ggaggccagc gagccctcct gggccgtgga ggaggcggcc
    10861 aatgcgttcg aggcgctcga tcgcatccgc gcgcaggcct tcgatgtcgc catcgtcgac
    10921 atctcgatgc ccggcatgag cgggctcgac ttcctcaagc gggtgcgcgc gtcgcagatg
    10981 accctgcgcg tgctgatgct gagcatgtac gcggaggagc agtacgccat gcgcgccttc
    11041 aagggcggtg ccaacggcta cgtcacgaag gattccgcga cgcgcgagct cgtgagcgcc
    11101 attcgcaagg tgacctccgg cagcgcctac gtcagcgaat cgctcgccca gagcctggcg
    11161 ctgggcctgg gcaatgcggc gggcgacgcc gcgcacgagc ggctgtcgga tcgggaactg
    11221 gacatcctgc ggcgactggc gaccggagac cggcccacgg agatcgcaca ggcgctccac
    11281 atctccgtga agaccgtcag cacccacaag gcccggatcc tggcccgcct gcaactgccg
    11341 aactcggccg ccctggtgcg ctacgcgatg gagcaccagc tgatcccgga aggatcgatg
    11401 cgcccgcgcg aggacttcgg aggcagcggc tggcagccgc tgtcggaaga cgatccagtg
    11461 gatccggagg ccggagcgtg agtggatcgg acgccccggc cacaggcaaa tccgtctccg
    11521 gcgggctgac gatccgcgcg cgcatcctgc tgctggcgct ggcggtgctc ctgcccgccg
    11581 cactggccct ggcgtggcgc ctcggcgacg agctccggga gtcccgggag gaagccttgc
    11641 agaccgtttc cctgttgcgg gacgactcga cccgccacct gtcctgggta cttcaacatg
    11701 cgtcctcgtt gctgggcttc gtcgccgcgc acgcgcggat gcgcgacggc gatgcggcgg
    11761 catgccaggc caccgtccag gccgtgccgc tgctgcaacc cggcttcatg cgcctggaga
    11821 tgcgtgacgc ctcgggccgg ctcgtctgct ggtcgagccg ccaggcgccg ccaacgctgc
    11881 ctccggacat ggacaggggt ccggtgacag gcctcgcagt gggtcgggtg cgggtcgatc
    11941 cgcccacggg acgcctcgtc gtgcccctgt cgcaggcggt ggtcgacgac gccggcgtcg
    12001 tccagggccg gctgcggctg ctgctggaca tgggcagcat gagcgaggag ctctcgcgca
    12061 acgccgccgc cggcgccggc gccgtgatca cggtgatcga tccggacgcc accatcctga
    12121 tgcgcaccca ccacgccgag gagttcatcg gcaagcgcgg gtcgattccc gatccgtcgg
    12181 gcggccaggg gaccggcttc atcgacggtg ccgggcggga cgggatcgac cggctctatg
    12241 cctacggcac ggttccagag gcaggctggc gcgtgttcgc cgggttgccg cgcgaccacg
    12301 tctacgccgg ctaccaggag acgcgccggc gcaccatcgc cgtcgggtcg atggtgctgg
    12361 cgctcgcgtg cgtgatggca tggcagctgg catcggtcat cgcccggccg atggcgtccc
    12421 tgcaggccgc ggcgcggcgc gtcggggccg gcgacctggg gcacgtgacc gtcgaagggc
    12481 cgccggagct cagggacgtc gccgaggact tcaaccacat ggtggatgcg ttgtcgctga
    12541 gccgctcgcg gctccaggcg ctgttcgaca cgatgtccga ggccgtcatc acggtcgacg
    12601 acgcccagac cgtcgtgatg gccaatccgg cagcggccac cctgctccgc tgcgcgatgc
    12661 ccgtgctgat cggctcgaag ctggatcgct ggctgccgga gcgggtgcgg gaagcgcacc
    12721 ggctcgacgt cgaacgcttc ggaacgagcg catccggacc gcgggacatg ggccaccgcc
    12781 ccgagatcag cgccctgcgc cacgacggcg aggagacgcc gatcgaggcg tcgatctcgc
    12841 tggtgcaagt cgagggccgg cggttctaca ccgcagtgct tcgcgacgtg agcgaacggc
    12901 gcaggacaat gaacgcgctg gcgcggaaca aggccttgct gaccgccgcc ctggccaaca
    12961 tgagcgacgc ggtcgccatc ctggacgcgc agggacgctt catcgccgtc aacgacgcct
    13021 tcacgacgtt ctaccgcctg cctcctggca gccccccgct cgcgaacgtc caggaactcg
    13081 ccgccctgct ggacatccgc cttcccgacg gccgcgcggc cggcccccac gagtgcgccg
    13141 gcctgcgggc gatcgcggga gagtcgggaa ccggcgtcct gtacaagctg cgtcgcctcg
    13201 atggtggcga cggatggacc ggcagtttca actttgcgcc gatccgcgac gcgcatggcg
    13261 ccatcacggg cgccgtctcc acggcgcgcg acgtcactgc acagctggcc gcgcagcacg
    13321 aactcgaaca ttcgcgcgac gcattgcggc gcctggtcgg ctc
//
LOCUS       DATCRO010000303         8815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_532085, whole genome shotgun sequence.
ACCESSION   DATCRO010000303 DATCRO010000000
VERSION     DATCRO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532085"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VIN75_08230"
     CDS             complement(<1..1318)
                     /locus_tag="VIN75_08230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8974191.1"
                     /translation="MTSAELIAQYGPREAMEYDVVIVGAGPAGLSTAIRLKQLAAEAG
                     KEVSVVVLEKGSEPGAHILSGAVMDPIAINELLPNWKADGGPLLQPVTGDEFLFLSET
                     GARSVPEWLMPMPFHNKGNYVISLGNVVKWLASQAEALGVEIFPGFAAAEVLYDDKGA
                     VKGVATGNVGVGRDGEPHDGFQLGMELHGKYTIFAEGARGHLGKQLISKFDLLAGRDA
                     QSWAIGIKELWEIPADKARPGHVVHTGGWPMMDDTFGGGFLYHLEGNKVTLGFVIGLD
                     YKNPWMSPFEEMQRWKTHPSIRKHLEGGKRLSYGARAINNGGLQCLPKLVFPGGALVG
                     CDAGTLNASRIKGSHAAIKTGMLAAEAAFAALQAGRQGDELAAYPQAFEKSWLHTELQ
                     RTKNFKLWIKKGKLIGNLMTGIEQWLMPKLGMIPSWTLHNTKKDNEA"
     gene            1530..2312
                     /locus_tag="VIN75_08235"
     CDS             1530..2312
                     /locus_tag="VIN75_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8974192.1"
                     /translation="MGMKIDLAGRVAFVTGASSGLGAQFAKTLADSGAGVVLAARRVE
                     RLKTLRAEIEAAGGDAHVVELDVTDPDSVKAAVAHAETEMGVIDILVNNSGVSTTQKL
                     VDVKPADFDYVFDVNAKGAFFVAQEVAKRMIARARGAAPGTYTGGRIVNVASMAGLRP
                     LSQIGIYAMSKASVIHMTRAMALEWGRYDINVNALCPGYIDTEINHHHWATEAGQKLI
                     QMTPRKRIGKPADLDSALLMLCANESHFINGAVLQADDGFGL"
     gene            2356..2781
                     /locus_tag="VIN75_08240"
     CDS             2356..2781
                     /locus_tag="VIN75_08240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8974193.1"
                     /translation="MRFDLPEGLKLVHEMRIPVRWGDLDAMGHVNNTIYFRYFESLRI
                     EWLKTLASEPNPEGVGPVMANGFCNFIRQIEYPGEIVARHYVVSPRQGAKSLDTYFTL
                     SMADAPDVVSANGGATLVWLDFPNRRTVPLPDHLRAACS"
     gene            complement(2789..3337)
                     /locus_tag="VIN75_08245"
     CDS             complement(2789..3337)
                     /locus_tag="VIN75_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8974194.1"
                     /translation="MRAGFDYRTAFEFAPIGLVLSRHRLMIDCNRAALAMFRAEREQL
                     IGQSFRVLYPTQGEYERTGERIIASLDTNGRYADERVMRRVDGELFWCHVWGHALDTA
                     DPHAAGIWSFEDLSSRRSLKLEFTPREREIAALLIEGLTSKMVGKRLNISPRTVDVYR
                     ARLMRKVGASTTAELVHKLLAA"
     gene            complement(3408..3815)
                     /locus_tag="VIN75_08250"
     CDS             complement(3408..3815)
                     /locus_tag="VIN75_08250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8974195.1"
                     /translation="MTIAELQALYAEVFAEWVRELELEVRAADGDTVTLALPVRARHV
                     HAGGVVCGQTLMAAADTALVLAISHRLGGFRPQTTVQLQTSFLRAVGQDVAEVAVQAR
                     ILKAGRSLVFGEVTVRLPDGQVAAHATSTSALL"
     gene            3965..4672
                     /locus_tag="VIN75_08255"
     CDS             3965..4672
                     /locus_tag="VIN75_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY8974196.1"
                     /translation="MNKVYPSAAAALQGIVKDGQLLAVGGFGLCGIPEALIDALRDSG
                     AKNLTVISNNAGVDGFGLGKLLATRQISKMISSYVGENKEFERQYLAGELQLEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTIVAEGKETREFNGQAYVMELALNPEVSLVKAWKA
                     DKSGNLMFRRTARNFNPNVAQAGKITVAEVEEIVETGTFDPDEVHLPGIFVHRIVLNA
                     HPEKRIEQRTVRKAATA"
     gene            4709..5347
                     /locus_tag="VIN75_08260"
     CDS             4709..5347
                     /locus_tag="VIN75_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY8974197.1"
                     /translation="MAWTRDEMAARAASELQDGFYVNLGIGLPTLVANHVPAGMEVWL
                     QSENGLLGIGPFPTEDAVDPDMINAGKQTVTTLPGSSIFSSADSFGMIRGGKINLAIL
                     GAMQVSEHGDLANWMIPGKMVKGMGGAMDLVAGVGRVVVLMEHVATKKDGSTDLKLIP
                     KCTLPLTGVGVVNRIITDLCVLDVTPQGLKLVELAPGVTRDEVQAKTGATLH"
     gene            complement(5557..6594)
                     /locus_tag="VIN75_08265"
     CDS             complement(5557..6594)
                     /locus_tag="VIN75_08265"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY8974198.1"
                     /translation="MSLVPYALTRPFLFGLDPEHAHEVTLDAIARLQNTPLQCLWGET
                     RVVDPVTVAGLNFPNRVGLAAGLDKNGRCIDGLGAMGFGFIEVGTVTPKAQPGNPKPR
                     MFRLPEANALINRLGFNNLGLQAFLDNVKRARSFRQSGGILGLNIGKNAATPIENATD
                     DYLIGLAGVYPHADYVTVNISSPNTKNLRALQSDEALDALLGALAERRAALARELGRQ
                     VPMFLKIAPDLDEAQVKVIAATLGRHGMDGVIATNTTISRDAVKGLRHAEETGGLSGA
                     PVLQASNDVIRQLRAELGSRFPIIGVGGVMSGADAKSKIDAGADLVQVYTGLIYQGAG
                     LVRDAALAVKR"
     gene            complement(6653..7282)
                     /locus_tag="VIN75_08270"
     CDS             complement(6653..7282)
                     /locus_tag="VIN75_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974199.1"
                     /translation="MNFVRIAACAALLAAAAGCTTPDYSPSALTPAQFSDPSRGVVLV
                     STGAVERCIGAAMWAPIYDARTEALVAGHPLLPIDATRQSDFPDHYGTLSALSLPPGR
                     YVITAEWANPVSEPTSRVPAWSFEVAAGESVYIGEIWRATPCQLSANVVLRDNYERDV
                     ALADKFNTRFVTQPPRKALAGSLHNDFVGRKTKDSIPERVQTHGQVMRQ"
     gene            7361..8029
                     /gene="rpiA"
                     /locus_tag="VIN75_08275"
     CDS             7361..8029
                     /gene="rpiA"
                     /locus_tag="VIN75_08275"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY8974200.1"
                     /translation="MTQDELKALVGQAAIAYVQPGSIVGVGTGSTVNHFIEALAKMPV
                     RVAGAVSSSVQSTERLKKLGIPVLEASEVTGLGVYVDGADEIDGRGCMIKGGGAALTR
                     EKIVADLADKFVCIADESKKVAVLGRFPLPVEVIQMAVAQVMRRFEAMGGQPVVRAGV
                     LTDNGHPIVDVRGLSITDPVALEREINQWPGVVCVGIFARHRANVCLLGTATGVQTLA
                     YDKA"
     gene            complement(8014..>8815)
                     /locus_tag="VIN75_08280"
     CDS             complement(8014..>8815)
                     /locus_tag="VIN75_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974201.1"
                     /translation="DEATRRAGFAAFATGTAASSGFGPFLAGQLMSRSQASTTFGVLA
                     AVSLAALAGASSTASRLQSTRATFAPTHAATATRSSLRGLGGLRRLLLADLGMALAWN
                     ANGFVVPIVVHAHGWPADIAGNLLGCFGAAVLLVRALPHAWRAPGSDWGAITRALLAS
                     GAVLALLPVLPSMGAHTLLQALLGCGLGSSLPSVMALVEARTPDGRRAEVLGLRQAVL
                     GFGAATTPTAVGALVTAAGLVPALAAVGGGLLVLGAAIAPRDASQALS"
BASE COUNT         1377 a         3026 c         3090 g         1322 t
ORIGIN      
        1 gtgcctcgtt gtccttcttc gtgttgtgca gcgtccagga cgggatcatg cccagcttgg
       61 gcatcagcca ctgctcgatg ccggtcatca ggttgccgat cagcttgccc ttcttgatcc
      121 acagcttgaa gttcttcgtg cgctgcagct cggtgtgcag ccagctcttc tcgaacgctt
      181 gcgggtaggc cgcgagttcg tcgccctggc ggccggcctg cagcgcggcg aacgcggcct
      241 cggccgccag catgcccgtc ttgatggcgg cgtggctacc cttgatgcgc gacgcgttga
      301 gcgtgccggc gtcgcaaccc accagcgcgc cacccgggaa cacgagcttg ggcaggcact
      361 gcaggccgcc gttgttgatg gcgcgggcgc cgtacgacag acgcttgccg ccctcgaggt
      421 gcttgcggat ggacgggtgc gtcttccagc gctgcatctc ctcgaacggg ctcatccacg
      481 ggttcttgta gtcgaggccg atcacgaagc cgagcgtgac cttgttgccc tcgaggtggt
      541 acaggaagcc gccgccgaag gtgtcgtcca tcatcggcca gccgccggtg tgcaccacgt
      601 ggcccggcct ggccttgtcg gccgggatct cccacagctc cttgatgccg atcgcccagc
      661 tctgggcgtc gcggccggcg agcaggtcga acttggagat cagctgcttg ccgaggtggc
      721 cgcgcgcgcc ttccgcgaag atcgtgtact tgccgtgcag ctccatgccc agctggaaac
      781 cgtcgtgcgg ctcgccgtca cgtcccaccc cgacgttgcc ggtggccacg cccttgacgg
      841 cgcccttgtc gtcgtacagc acctccgccg cggcgaagcc ggggaagatc tcgacgccca
      901 gcgcctcggc ctggctcgcc agccacttga cgacgttgcc gaggctgatg acgtagttgc
      961 ccttgttgtg gaagggcatg ggcatcagcc attcgggcac gctcctggcg cccgtctcgg
     1021 acaggaacag gaactcgtcg ccggtcaccg gctgcagcag cgggccgccg tcggccttcc
     1081 agttcggcag cagctcgttg atcgcgatcg gatccatcac cgcgccggac aggatgtgcg
     1141 cgcccggctc ggaacccttc tcgagcacca cgaccgacac ctccttgccg gcctccgcgg
     1201 cgagctgctt caggcggatc gcggtggaca gtccggccgg gccggcgccg acgatcacca
     1261 cgtcgtattc catcgcctcg cgcgggccgt attgagcaat gagttctgcc gaagtcatgg
     1321 ggagcgttcc tggggaaggg gagatcctgg ggcgccggtc ggagccgatg gacaggcatt
     1381 ctaagggcgt gccacgagaa aatagcacga tcgttcgatt tttatcgtgc gtgctatggt
     1441 ggcatccatc taatccgggc gcggccttgt cgcacaggcg actcgaggcc gtcgctccgg
     1501 cgcgaaacga acacaggagc gagaagatca tgggcatgaa gatcgacctg gcgggccgcg
     1561 tggccttcgt caccggcgcg tccagcggac tgggtgcgca attcgccaag acgctggccg
     1621 actccggcgc gggcgtcgtg ctggccgcgc gccgcgtcga gcgcctgaag acgctgcgcg
     1681 ccgagatcga ggccgccggc ggcgacgccc acgtcgtcga gctcgatgtc accgaccccg
     1741 acagcgtcaa ggccgccgtc gcgcacgccg agacggagat gggcgtcatc gacatcctgg
     1801 tcaacaactc cggcgtcagc accacgcaga agctggtcga cgtcaagccc gcggacttcg
     1861 actacgtgtt cgacgtcaac gccaagggcg cgttcttcgt cgcccaggaa gtcgccaagc
     1921 gcatgatcgc ccgcgcccgc ggcgccgcgc cgggcacgta taccggcggc cgtatcgtca
     1981 acgtcgcctc gatggccggc ctgcgcccgc tgtcgcagat cggcatctac gcgatgagca
     2041 aggcctcggt gatccacatg acgcgcgcca tggcgctcga gtggggccgc tacgacatca
     2101 acgtcaacgc gttgtgcccc ggctacatcg acaccgagat caaccaccac cactgggcca
     2161 ccgaggccgg ccagaagctg atccagatga cgccccgcaa gcgcatcggc aagcccgccg
     2221 acctcgacag cgcgctgctg atgctgtgcg ccaacgagag ccatttcatc aacggcgcgg
     2281 tgctccaggc cgacgacggc ttcgggttgt gacagccccc gcgctcctgc cgtcgctgcc
     2341 cgggttctct tcgacatgag attcgacctc cccgaaggat tgaagctcgt ccacgagatg
     2401 cgcatccccg tgcgctgggg cgacctcgac gcgatgggcc acgtcaacaa cacgatctac
     2461 ttccgctact tcgagtcgct gcgcatcgag tggctgaaga cgttggcctc cgagcccaat
     2521 cccgagggcg tcggcccggt gatggccaac ggcttctgca acttcatccg ccagatcgag
     2581 taccccggcg agatcgtggc gcgccactac gtcgtctcgc cgcgacaggg cgccaagagc
     2641 ctggacacct acttcacact gtccatggcc gatgcgcccg acgtggtgtc tgccaacggc
     2701 ggcgcgacgc tggtgtggct ggacttcccg aacaggagga cggtgccgct gcccgatcac
     2761 ctgcgcgcgg cgtgcagttg atggagcctc acgccgccag cagcttgtgc accagctccg
     2821 cggtggttga cgcgcccacc ttgcgcatca gccgcgcccg gtagacgtcg acggtgcgcg
     2881 ggctgatgtt gagccgcttg ccgaccatct tgctggtcag cccctcgatc agcagcgcgg
     2941 cgatctcgcg ttcgcgcggg gtgaactcga gtttcagcga acgcctggac gacaggtcct
     3001 cgaagctcca gatgcccgcg gcgtgcgggt cggcggtgtc gagcgcgtgg ccccacacgt
     3061 ggcaccagaa cagctcgccg tcgacgcggc gcatcacgcg ctcgtcggcg tagcggccgt
     3121 tggtgtccag gctcgcgatg atgcgttcgc cggtgcgctc gtattcgccc tgggtcgggt
     3181 agagcacgcg gaacgactgg ccgatcagct gttcgcgctc ggcgcggaac atcgcgagcg
     3241 cggcccggtt gcagtcgatc atcagccggt ggcgcgacag cacgaggccg atgggcgcga
     3301 actcgaacgc ggtgcggtag tcgaagcccg cgcgcaggtc ggcggcgtgg gccagggcct
     3361 ccggcgaggc cacgggcgga tgcagcgcgt cgcggctcac gcgagcctca cagcagcgcc
     3421 gacgtgctgg tcgcgtgcgc ggccacctgg ccgtcgggca ggcgcacggt cacctcgccg
     3481 aacaccaggc tgcggccggc cttgaggatg cgggcctgga cggcgacctc ggccacgtcc
     3541 tggcccaccg cgcgcaggaa gctggtctgc agctgcacgg tggtctgcgg tcggaagccg
     3601 ccgagcctgt ggctgatggc gagcaccagc gccgtgtcgg cggctgccat cagggtctgg
     3661 ccgcacacga cgccgcccgc gtgcacgtga cgcgcgcgca cgggcagggc gagcgtgacg
     3721 gtgtcgccgt cggcggcgcg cacctcgagc tcgagttcgc gcacccattc ggcgaagact
     3781 tcggcgtaga gtgcctgcag ttcggcgatc gtcattcgaa tcgtccctcc ggggcgtcgc
     3841 gggcccctga tagtcaattc tacgtaatgg ctacgtaacg ccttaagtag tacgcttccg
     3901 catcgacgca ccggatcagg ctccgggctc acgcatctcg aatttccacc ggagacaagc
     3961 gcgaatgaac aaggtctatc cgagcgccgc cgcggcgctt caaggcatcg tcaaggacgg
     4021 gcaactgctc gccgtcgggg gcttcggcct ctgcggcatc cccgaggccc tgatcgacgc
     4081 gctgcgtgac agcggtgcga agaacctgac ggtcatctcg aacaacgcgg gcgtcgacgg
     4141 cttcggcctc ggcaagctgc tggccacgcg ccagatctcc aagatgatct ccagctacgt
     4201 cggcgagaac aaggagttcg agcgccagta cctggccggc gaactgcagc tggagttcac
     4261 gccgcagggc acgctggccg agaagctgcg cgccggtggc gccggcatcc cggcgttctt
     4321 cacgaagacg ggcgtgggca ccatcgtggc cgaaggcaag gagacgcgcg agttcaacgg
     4381 ccaggcctac gtgatggagc tggcgctgaa ccccgaggtg tcgctcgtga aggcgtggaa
     4441 ggccgacaag tcgggcaacc tgatgttccg ccgcaccgcg cgcaacttca atccgaacgt
     4501 ggcgcaggcc ggcaagatca ccgtcgccga ggtcgaggag atcgtcgaga cgggcacgtt
     4561 cgaccccgac gaggtccacc tgccgggcat cttcgtgcac cgcatcgtgc tgaacgcgca
     4621 ccccgagaag cgcatcgagc agcgcacggt ccgcaaggcg gcgaccgcct gaatccaccc
     4681 gaacacacga attcccggag caacacgaat ggcctggacc cgagacgaga tggcggcgcg
     4741 tgccgcgagc gaactgcagg atggtttcta cgtgaacctg ggcatcggcc tgcccacgct
     4801 ggtcgccaac cacgtgcccg cgggcatgga ggtgtggctg cagagcgaga acggcctgct
     4861 gggcatcggc ccgttcccga ccgaggacgc ggtcgacccc gacatgatca acgccggcaa
     4921 gcagacggtg accacgctgc cgggctccag catcttctcc agcgcggaca gcttcggcat
     4981 gatccgcggt ggcaagatca acctggccat cctgggcgcg atgcaggtca gcgagcacgg
     5041 cgacctcgcc aactggatga tccccggcaa gatggtcaag ggcatgggcg gcgcgatgga
     5101 cctcgtcgcc ggcgtcggcc gcgtcgtcgt gctgatggag cacgtggcca cgaagaagga
     5161 cggttcgacg gacctgaagc tgatccccaa gtgcacgctg ccgctgaccg gcgtgggcgt
     5221 ggtcaatcgc atcatcaccg acctgtgcgt gctggacgtg acgccgcaag gcctcaagct
     5281 cgtggagctg gcgccgggcg tgaccaggga cgaggtgcag gccaagacgg gagcgacgct
     5341 gcactgaggc gcgcgacgcg ccgatgacga aggggccgcg cggccccttt gtcgcttctc
     5401 gtgggggcct ggcgtggtcg aaggggtctg gcctctcctc gaatcgcgcc gtttggtcga
     5461 tcaaggccat gccgctgatc gcggttcgag gcgaaggagg gcctccgctt cgatcgccga
     5521 tgcgccgcgg atgaaggaga ggccagaccc ctccagtcag cgcttgacgg ccagcgccgc
     5581 gtcgcgcacc agccccgctc cctggtagat cagccccgtg tagacctgca ccaggtcggc
     5641 gccggcgtcg atcttggact tcgcatcggc gccggacatc acgccgccca cgccgatgat
     5701 ggggaagcgg ctgccgagtt cggcgcgcag ctggcggatg acgtcgttgc tcgcctgcag
     5761 caccggcgca ccggacaggc cgcccgtctc ctcggcatgg cgcaggccct tgaccgcgtc
     5821 gcgcgagatc gtcgtgttgg tggcgatgac gccgtccatg ccgtgccggc ccagcgtggc
     5881 ggcgatgacc ttgacctgcg cttcatcgag atcgggcgcg atcttcagga acatcggcac
     5941 ctggcggccg agctcgcgcg ccagcgcggc gcggcgctcg gccagtgcgc ccagcagcgc
     6001 gtcgagcgcc tcgtcgctct gcagcgcgcg caggttcttg gtgttggggc tggagatgtt
     6061 gaccgtcacg tagtcggcgt gcgggtagac gccggccagg ccgatcaggt agtcgtcggt
     6121 cgcgttctcg atcggcgtcg ccgcgttctt gccgatgttc aggcccagga tgccgcccga
     6181 ctggcggaag ctgcgcgcgc gcttgacgtt gtccaggaag gcctgcaacc ccaggttgtt
     6241 gaagcccagc cggttgatca gcgcgttggc ctcgggcagg cggaacatgc gcggcttggg
     6301 gttgccgggc tgtgccttgg gcgtgacggt gcccacctcg atgaagccga agcccatcgc
     6361 gcccaggccg tcgatgcagc ggccgttctt gtccaggccg gcggccaggc ccacgcggtt
     6421 ggggaagttc aggccggcga ccgtcaccgg gtcgacgacg cgcgtctcgc cccaaaggca
     6481 ttgcagcggc gtgttctgca gccgcgcgat cgcgtccagc gtcacctcat gggcgtgctc
     6541 ggggtcgagg ccgaacagga acgggcgggt gagggcgtag gggacgagcg acatggaacg
     6601 gctggagcgg tgggcgatgc gaagcgccta ttgtcggtca tggccccacg tctcattgac
     6661 gcatgacctg gccgtgcgtc tgcacccgct cggggatcga gtcctttgtc ttgcggccca
     6721 cgaagtcgtt gtgcaggctg ccggcgagcg ccttgcgagg cggctgggtg acgaatcgcg
     6781 tgttgaactt gtctgccagc gccacgtcgc gctcgtagtt gtcgcgcagc accacgttgg
     6841 cggaaagctg gcagggcgtg gcgcgccaga tctcgccgat gtagacggac tcgcccgcgg
     6901 cgacctcgaa cgaccaggcc ggcacccggc tggtgggttc ggagaccgga ttggcccact
     6961 ccgcggtgat cacgtagcgg ccgggcggca gcgagagcgc gctcagcgtg ccgtagtggt
     7021 cggggaagtc gctctggcgg gtggcgtcga tcggcagcag cggatggccg gccaccaggg
     7081 cctcggtgcg ggcgtcgtag atcggcgccc acatcgccgc gccgatgcag cgctcgacgg
     7141 ctccggtgct caccagcacg acgccgcgcg acgggtcgga gaattgcgcc ggggtcagcg
     7201 cgctcggcga gtagtcgggc gtggtgcagc cggcggccgc ggcgagcaag gcggcacagg
     7261 cggcgattcg gacgaagttc atggacgcac gggtcgtgga agtggccgta ttgtcgcggc
     7321 aacggtcgcc cgcgcgatcg gggccagcga taatcggcgg atgacccaag acgaactcaa
     7381 ggccctcgtc ggccaggccg ccatcgccta cgtccaaccc ggcagcatcg tcggcgtggg
     7441 gacgggctcg accgtgaatc acttcatcga ggcgctggcg aagatgccgg tgcgcgtggc
     7501 gggcgccgtc tccagctccg tccagagcac ggagcgcctg aagaagcttg gcatccccgt
     7561 gctggaagcg agcgaggtga cgggcctggg cgtgtacgtc gacggcgccg acgagatcga
     7621 cggccggggc tgcatgatca agggcggcgg cgcggccctg acgcgcgaga agatcgtcgc
     7681 cgacctcgcg gacaagttcg tctgcatcgc cgacgaatcc aagaaggtcg ccgtgctggg
     7741 ccgcttcccg ctgcccgtcg aggtgatcca gatggccgtg gcgcaggtga tgcgccgctt
     7801 cgaggcgatg ggcgggcagc ccgtcgtgcg cgccggcgtg ctgaccgaca acggccaccc
     7861 gatcgtcgac gtgcgcgggc tgtccatcac cgacccggtg gcgctggagc gcgagatcaa
     7921 ccaatggccc ggcgtcgtct gcgtgggcat cttcgcgcgc catcgcgcca atgtgtgcct
     7981 gctgggcacc gccaccggcg tgcagacgct cgcctacgac aaggcctgag acgcgtcgcg
     8041 cggcgcgatc gcggcaccca gcaccagcag gccgccgccg accgccgcca gcgccggcac
     8101 cagccctgcg gccgtgacca gcgcgcccac cgcggtgggc gtcgtcgcgg cgccgaaccc
     8161 gagcacggcc tggcgcaacc cgagcacctc cgcgcgccgg ccgtccggcg tgcgcgcctc
     8221 caccagcgcc atcaccgacg gcagtgagct gcccaggccg cagcccagca gcgcctgcag
     8281 cagggtgtgc gcccccatcg acggcagcac cggcagcagc gcgagcaccg cgccgctggc
     8341 cagcagcgcg cgcgtgatcg cgccccagtc gctgcccggc gcgcgccacg cgtgcggcaa
     8401 cgcgcgcacg agcagtaccg ccgcgccaaa gcagcccagc aggttgccgg cgatgtccgc
     8461 cggccagccg tgcgcatgca cgacgatcgg cacgacgaag ccgttcgcgt tccacgccag
     8521 cgccatgccc aggtcggcca gcagcaggcg gcgcaggccg cccaggccgc gcagcgagga
     8581 gcgcgtcgcc gtggccgcgt gcgtcggcgc gaacgtggcg cgcgtcgatt gcagccgcga
     8641 ggcggtcgag gacgcaccgg ccagcgccgc cagcgacacg gccgccagca cgccgaaggt
     8701 cgtcgacgcc tgcgagcgcg acatgagctg ccccgccagg aagggcccga acccgctcga
     8761 ggccgcagtg ccggtcgcga aggccgcgaa gcccgcgcga cgcgtggcct cgtcg
//
LOCUS       DATCRO010000304         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_475851, whole genome shotgun sequence.
ACCESSION   DATCRO010000304 DATCRO010000000
VERSION     DATCRO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475851"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VIN75_08285"
     CDS             complement(<1..292)
                     /locus_tag="VIN75_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974202.1"
                     /translation="MRADRPAPATDAAARRWTLVSAVLGSGMAFMDGTVVNVALPAIQ
                     RGMDASGADAQWVIEAYALFLAALLLVGGALDDRFGRKRVFLVGAAVFTLASA"
     gene            407..724
                     /locus_tag="VIN75_08290"
     CDS             407..724
                     /locus_tag="VIN75_08290"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY8974203.1"
                     /translation="MRKLLLALLLPFVFLLSQQGAVTHELSHLTDAVSRAAGGPGEPG
                     HQPGDAQCEACLAFAHLVGVATAQVLPPALLSFDFGIPASEAPAFIAADAPAARSRGP
                     PVL"
     gene            887..>2768
                     /locus_tag="VIN75_08295"
     CDS             887..>2768
                     /locus_tag="VIN75_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8974204.1"
                     /translation="MFGFNHRDVARALVLSAPLSVALSALADEAAAPAAAASAPPFPR
                     TQLASAADAAQTVIVDAVRQKLDAARNGLSPDTGSSVYKFGEADIDALPLGQATPLNQ
                     VVLQAPGVVQDSYGQLHVRGDHANVQYRIDGVVIPEAISGFGQALETRLADNIAVLTG
                     ALPAQYGYRTAAVVDIKTRQQAQGDSGSVSYTGGSNAHNEGSGEIGGAHGPISWYMTG
                     SLLRDNMGIENPTADRNALHDKTHQAKGFGTVSYLLGDDSRLNLMFGVSNNRFQIPDV
                     PGQAPQYSLAGDASVDSATLDARQREHDAFEVLSYQGHAGAALDYQLSLFHRETDVHY
                     APDPVGDLVFDGIAADIRRKNTADGLQLDSSYALGKSHTLRGGLFLQHEKFGVDNQSQ
                     VFPADAEGNQTSTTPIGITDDTSIHGNLWGAYLQDEWQPVEGLTVNYGARFDDVDTVV
                     KEHQLSPRLGLVFDAAPTLRLHAGYARYFTPPPTEKIDTTSVAKFAGTTNALPSDADT
                     SVRSERSNYFDAGLAWDATPELSLGLDAYDREVRNLQDEGQFGNALVYSAFNYAKGRV
                     RGLELTANYRHDAFSAYGNLAVSKAQGKQIVTGQFNFDADEIDYIASHWVHLDHDQAV
                     TAS"
BASE COUNT          422 a         1062 c          917 g          367 t
ORIGIN      
        1 ccgccgacgc cagcgtgaac accgcggcgc ccacgaggaa cacgcgcttg cgcccgaagc
       61 ggtcgtcgag agcgccgccc accagcagca gcgcggccag gaagagcgcg taggcctcga
      121 tcacccactg ggcgtccgcg ccgctcgcgt ccatgccccg ctggatcgcg ggcaacgcga
      181 cgttgacgac ggtgccgtcc atgaacgcca tgcccgagcc gagcaccgcg ctgaccagcg
      241 tccagcggcg cgccgcggcg tcggtggcgg gagcagggcg gtcggcgcgc atgcgggcat
      301 tctcgtccgg ggcgaatgtc cctgcgggcg cgttcgcgcg tgcgggggcc gcggccatgt
      361 ccctcgacag ccccgggtca ggcgttccct ccataatcga cggtccatgc gcaagctgct
      421 gctcgccctc ctgctgccgt tcgtcttcct gctctcgcag cagggcgcgg tcacgcacga
      481 gctgagccac ctgaccgacg ccgtgtcccg cgcggccggc ggtccgggcg agcccggcca
      541 ccagcccggc gatgcgcaat gcgaggcctg cctggccttc gcgcacctgg tcggcgtggc
      601 caccgcgcag gtgctgccgc ccgcgctgct gagcttcgac ttcggcatcc cggccagcga
      661 ggcgccggcg ttcatcgccg ccgatgcgcc cgccgcgcgc agccgcggcc cgccggtcct
      721 ttgagccccc tcgctcgcta gcgctcgctg ccccccgagg gggccggccg cgacgcgccc
      781 ggcaaagccg gttcgcgcgc tcccttgcac ttcgtgcgtc tgcccgagcg gcagcgccgg
      841 gtgcttgcgt gcgccgacgt ctcatccatc ttgaaggatc tctcacatgt tcggtttcaa
      901 tcaccgggac gtggcgcgcg cgctcgttct gtccgcaccc ctgtcggtcg ccctgtccgc
      961 ccttgccgac gaggccgccg cgcccgccgc cgcggcctcg gcgccgccgt tcccccgcac
     1021 ccagctggct tcggccgccg atgccgcgca gaccgtcatc gtcgacgccg tgcgccagaa
     1081 gctcgacgcc gcgcgcaacg ggctgtcgcc ggacaccggc agcagcgtct acaagttcgg
     1141 cgaggccgac atcgacgcgc tgccgctggg ccaggccacg ccgctgaacc aggtggtgct
     1201 gcaggcgccc ggcgtcgtgc aggactcgta tggccagctg cacgtgcgcg gcgaccacgc
     1261 caacgtgcag taccgcatcg acggcgtcgt catccccgag gccatcagcg gcttcggcca
     1321 ggcgctggag acgcgcctgg ccgacaacat cgcggtgctc accggcgcgc tgccggcgca
     1381 gtacggctac cgcaccgcgg ccgtggtcga catcaagacc aggcagcagg cccagggcga
     1441 ctcgggcagc gtcagctaca ccggcggcag caacgcgcac aacgagggca gcggcgagat
     1501 cggcggcgcg cacggcccga tcagctggta catgaccggc tcgctgctgc gcgacaacat
     1561 gggcatcgag aaccccacgg ccgaccgcaa cgcgctgcac gacaagacgc accaggccaa
     1621 gggcttcggc accgtctcgt acctgctggg cgacgacagc cgcctgaacc tcatgttcgg
     1681 cgtgtcgaac aaccgcttcc agatccccga cgtgcccggc caggcgccgc agtattcgct
     1741 cgcgggcgac gcctcggtcg actccgcgac gctggacgcg cgccagcgcg agcacgacgc
     1801 cttcgaggtg ctgtcgtacc agggccatgc gggcgccgcg ctggactacc agctgtcgct
     1861 gttccaccgc gagaccgatg tgcactacgc gcccgacccg gtcggcgatc tcgtcttcga
     1921 cggcatcgcg gccgacatcc gccgcaagaa cacggccgac ggactgcagc tggactcgag
     1981 ctacgcgctc ggcaagtcgc acacgctgcg cggcggcctg ttcctgcagc acgagaagtt
     2041 cggcgtcgac aaccagtcgc aggtcttccc ggccgacgcc gagggcaacc agacgagcac
     2101 gacgccgatc ggcatcacgg acgacacctc catccacggc aacctgtggg gcgcgtacct
     2161 gcaggacgaa tggcagccgg tcgagggcct gaccgtcaac tacggcgcgc gcttcgacga
     2221 cgtcgacacc gtcgtcaagg agcaccagct cagcccgcgc ctgggcctgg tgttcgacgc
     2281 ggcgccgacg ctgcgcctgc acgccggcta cgcgcgctac ttcacgccgc cgcccaccga
     2341 gaagatcgac accacctcgg tggccaagtt cgccggcacc accaacgcgc tgccgtccga
     2401 cgccgacacg tcggtgaggt ccgaacgctc gaactacttc gacgccggcc tcgcctggga
     2461 cgccacgccc gagctgtcgc tgggtctcga cgcctacgac cgcgaggtgc gcaacctgca
     2521 ggacgagggg cagttcggca acgcgctggt ctactcggcc ttcaactacg cgaagggacg
     2581 cgtgcgcggc ctggagctga cggcgaacta ccggcacgac gcgttcagcg cctacggcaa
     2641 cctggccgtc tcgaaggcgc aaggcaagca gatcgtcacc ggccagttca acttcgatgc
     2701 cgacgagatc gactacatcg ccagccactg ggtgcatctc gaccatgacc aggccgtgac
     2761 ggcgtcgg
//
LOCUS       DATCRO010000305         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1094948, whole genome shotgun sequence.
ACCESSION   DATCRO010000305 DATCRO010000000
VERSION     DATCRO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094948"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1510)
                     /gene="glmU"
                     /locus_tag="VIN75_08300"
     CDS             complement(14..1510)
                     /gene="glmU"
                     /locus_tag="VIN75_08300"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8974205.1"
                     /translation="MALSVVIMAAGKGTRMRSARPKVLHTLGGKPLLGHVVATAEALS
                     PGKCIVVTGHGAEAVEAAFEGAQRVFVRQMPQLGTGHAVQQAVPALTDDEGVTLVLNG
                     DVPLIREATLRALVEACAGKNLALLTVDLDNPFGYGRIVRTPEAGDADGIGAVQAIVE
                     EKDATPLQRQITEVYTGILAAPTKRLKGWLARLTDDNAQREFYLTDVVAHAVSDGVEV
                     RTARAQDETEVLGVNSPMQLSDLERRLQRRHADALMEAGVRLADPARFDLRGTLACGM
                     DVEIDVGCVFEGDVKLGDNVHVGPHCVIRDAEIAAGAVIHPFTHIEGATVGAGTLVGP
                     FARLRPGAKLGEEVHIGNFVEVKNSTLANGAKANHLAYLGDATVGERVNYGAGSITAN
                     YDGANKHRTVIGADVHVGSNCVLVAPVELGAGATIGGGSTITKTVAPGSLAIGRARQV
                     AFENWERPFVCLRSPSAQTKGAPRSAFFAWAVPVSGRTGCGPAPVRRR"
     gene            complement(1846..2547)
                     /locus_tag="VIN75_08305"
     CDS             complement(1846..2547)
                     /locus_tag="VIN75_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY8974206.1"
                     /translation="MNSILFLRRMAAVVALGAAAALAGCASMNVLDAQVATFGAWPAG
                     ATPGTYAFDRLPSQEKNPQRTQAIENAAAQALANAGFHPVAEGGKPAVMVQIGARTER
                     FEQAPWDDPFWWGGPRRWGYRGWAGYGGPYGPWGPYGYHHGLWAPFPPEPDVYLHEVA
                     LLIRDADTGKALYEARASTDGYSSGGDRMLAAMFDASMKDFPNTNDKAHTIRVPLPMV
                     PSALPVAPAASAPRA"
     gene            complement(2730..3671)
                     /gene="ttcA"
                     /locus_tag="VIN75_08310"
     CDS             complement(2730..3671)
                     /gene="ttcA"
                     /locus_tag="VIN75_08310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY8974207.1"
                     /translation="MSLEEPRFTADAAAAAPDAQGEAARKKHAYEINKLSKRLHRQVG
                     AAIIDFNMIEEGDKVMVCLSGGKDSYAMLDILLSLRERSPVKFELVAVNLDQKQPGFP
                     EHVLPDYLRSRGVPFHIEEQDTYSIVTKHIEPGKTMCSLCSRLRRGILYRVASELGCT
                     KIALGHHRDDIVVTMFMNMFFGSRMKGMPAKLVSDDGKNVVIRPLAYVDETDLVRWAE
                     HRQFPIIPCTLCGSQDNLQRVQVKAMIREWEKQYPGRIENVFTAMANITPSHMMDRKL
                     HPFTTIRASGRPEPDGDIAFDEDETCGTPRPGVIRIT"
     gene            complement(3668..>3791)
                     /locus_tag="VIN75_08315"
     CDS             complement(3668..>3791)
                     /locus_tag="VIN75_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8974208.1"
                     /translation="LALMLAHPNVRAARVSTEKPDVYPDCDSVGVEVFGLKDAA"
BASE COUNT          501 a         1311 c         1366 g          613 t
ORIGIN      
        1 gccgaagagg cgctcatcga cgccgaactg gcgccggccc gcagccagtt ctgccactga
       61 cgggcaccgc ccacgcaaaa aaggcgctcc gaggagcgcc ttttgtttgg gcagacggcg
      121 atctcaagca aacgaagggc cgctcccagt tctcgaacgc cacctgccgc gcgcggccga
      181 tcgccagcga gccgggggcc accgtcttgg tgatcgtcga gccgccgccg atggtggcgc
      241 cggcgcccag ttcgaccgga gcgaccagca cgcagttcga gcccacgtgc acgtcggcgc
      301 cgatgacggt gcggtgcttg ttggcgccgt cgtagttggc cgtgatgctg cccgcgccgt
      361 agttgacgcg ctcgccgacg gtcgcgtcgc ccaggtaggc caggtggttg gccttggcgc
      421 cgttggccag cgtcgagttc ttgacctcga cgaagttgcc gatgtgcact tcctcgccca
      481 gcttcgcgcc cgggcgcagg cgagcgaacg ggccgaccag cgtgcccgcg cccaccgtcg
      541 cgccctcgat gtgcgtgaaa ggatggatca ccgcgccggc ggcgatctcg gcgtcgcgga
      601 tgacgcagtg cgggccgacg tgcacgttgt cgcccagctt gacgtcgccc tcgaacacgc
      661 agccgacgtc gatctcgacg tccatgccgc aggccagcgt gccgcgcagg tcgaagcgcg
      721 ccgggtcggc caggcgcacc ccggcttcca tcagtgcgtc ggcatggcgg cgctgcaggc
      781 ggcgctcgag gtcggacagc tgcatcgggc tgttgacgcc cagcacctcg gtctcgtcct
      841 gggcgcgggc cgtgcgcacc tccacgccgt cggacaccgc atgcgcgacg acgtcggtca
      901 ggtagaactc gcgctgcgcg ttgtcgtcgg tcaggcgggc gagccagccc ttgagccgct
      961 tggtgggcgc ggccaggatg ccggtgtaga cctcggtgat ctggcgctgc agcggcgtgg
     1021 cgtccttctc ctcgacgatg gcctggacag cgccgatgcc gtcggcatcg ccggcttccg
     1081 gcgtgcgcac gatgcggccg tagccgaacg ggttgtccag gtcgacggtc agcagcgcaa
     1141 ggttcttccc ggcgcaggcc tcgaccagcg cgcgcagcgt ggcctcgcgg atcagcggca
     1201 cgtcgccgtt gagcaccagc gtgacgccct cgtcgtcggt cagcgccggg acggcctgct
     1261 gcacggcgtg gccggtgccc agctgcggca tctggcgcac gaacacgcgc tgcgcgccct
     1321 cgaacgcggc ctcgaccgcc tccgcgccat ggcccgtgac gacgatgcac ttgccgggcg
     1381 acagggcctc ggcggtggcc accacgtggc ccagcagcgg cttgccgccc agcgtgtgca
     1441 gcaccttggg tcgcgcggag cgcatccgcg tgcccttgcc cgccgccatg atcacgacac
     1501 tgagtgccat aggagattcc ggttcgtctg aggaagcgga attatcgcaa gccggggcgg
     1561 cggcccggtg accccctgtc gggggagcct gcggggaaaa ttgcacgccg cgagggcttt
     1621 cgcggcggcg agaggggtgt caggtctcgc cttcgcgcgc tcggccgctt cccttggggt
     1681 ccgcttccct tggggtcagg tcttgaaagt tgcgcgctgc gcttcccttg gggtcaggtc
     1741 ttgaaagttg cgcgacgacc tgccggcgac cgacatgcgg tacagcgcgc aactttcaag
     1801 acctgacccc gatgcaggac ctgacacccg gcgccgcccg gctgttcagg cgcggggcgc
     1861 gctcgccgcc ggggcgaccg gcagcgccga cggcaccatc ggcagcggca cgcggatcgt
     1921 gtgggccttg tcgttggtgt tcgggaagtc cttcatcgag gcgtcgaaca tggcggccag
     1981 catgcggtcg ccgccggagc tgtagccgtc ggtgctggcg cgcgcctcgt acagcgcttt
     2041 gccggtgtcg gcgtcgcgga tcagcagcgc cacctcgtgc aggtagacgt cgggctcggg
     2101 cgggaacggg gcccagaggc cgtggtggta gccgtagggg ccccaggggc cgtacgggcc
     2161 gccgtagccg gcccagccgc ggtagcccca gcggcgcggg ccgccccacc agaacgggtc
     2221 gtcccagggc gcctgctcga agcgctcggt gcgcgcgccg atctgcacca tcaccgccgg
     2281 cttgccgcct tcggcgaccg ggtggaagcc ggcgttggcc agggcctgcg ccgcggcgtt
     2341 ctcgatggcc tgcgtgcgtt gcgggttctt ctcctgcgac ggcaggcggt cgaaggcgta
     2401 ggtgcccggc gtggcgccgg cgggccaggc gccgaaggtc gcgacctggg cgtcgagcac
     2461 gttcatgctg gcgcagccgg cgagcgccgc ggcggcgccg agcgcgacga ccgcggccat
     2521 gcgacggagg aagaggatgc tgttcatgac ggactcccgg gtgccggtcg gcggcaccgc
     2581 aatgccgagc ttcgcgctcg tgtgtaactc gagtatgtac cggcacgcgg cgcgcccgcc
     2641 gggccgctgt ccgatggctg tagccagcct tgcggcagcg ggggcggtcc tcgtcgattc
     2701 cgcgagcgcc gcgcgcggtt caacccgcgt caggtgatgc ggatcacgcc cggcctgggc
     2761 gtgccgcagg tctcgtcctc gtcgaacgcg atgtcgccgt ccggctcggg ccggccgctc
     2821 gcccggatcg tcgtgaacgg atgcagcttg cggtccatca tgtgcgacgg cgtgatgttg
     2881 gccatcgcgg tgaagacgtt ctcgatgcgg ccggggtact gcttctccca ctcgcggatc
     2941 atcgccttga cctgcacgcg ctgcaggttg tcctggctgc cgcacagcgt gcacgggatg
     3001 atcgggaact ggcggtgctc cgcccagcgc acgaggtcgg tctcgtcgac gtaggccagc
     3061 ggccggatga cgacgttctt gccgtcgtcg ctcacgagct tggccggcat gcccttcatg
     3121 cggctgccga agaacatgtt catgaacatc gtcacgacga tgtcgtcgcg gtggtggccc
     3181 agcgcgatct tggtgcagcc cagctcgctg gccacccggt acaggatgcc gcggcgcagg
     3241 cgcgagcaca gcgagcacat cgtcttgccc ggctcgatgt gcttggtcac gatcgagtac
     3301 gtgtcctgct cctcgatgtg gaacggcacc ccgcgcgagc gcaggtagtc gggcagcacg
     3361 tgctccggga agccgggctg cttctggtcg aggttgacgg cgaccagctc gaacttcacg
     3421 ggcgagcgct cgcgcagcga cagcaggatg tccagcatcg cgtagctgtc cttgccgccg
     3481 gacaggcaca ccatgacctt gtcgccttcc tcgatcatgt tgaagtcgat gatggcggcg
     3541 ccgacctggc ggtgcaggcg cttggacagc ttgttgatct cgtacgcgtg cttcttgcgc
     3601 gcggcctcgc cctgcgcgtc gggcgcggcg gcggccgcgt cggccgtgaa cctgggttcc
     3661 tcgaggctca tgctgcgtcc ttcaggccga acacttccac gcccaccgaa tcgcagtccg
     3721 ggtagacgtc gggcttttcc gtcgacaccc gcgccgcccg cacgttcggg tgcgccagca
     3781 tcagcgccag c
//
LOCUS       DATCRO010000306         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_734729, whole genome shotgun sequence.
ACCESSION   DATCRO010000306 DATCRO010000000
VERSION     DATCRO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734729"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VIN75_08320"
     CDS             <1..219
                     /locus_tag="VIN75_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974209.1"
                     /translation="VLPSPAWIAGCVLFSLVGLAAWRYGKAVERPAIRWLGLALMLYS
                     YFTAPTWLLYGVGLALCAAIWWNRPRAD"
     gene            complement(224..649)
                     /locus_tag="VIN75_08325"
     CDS             complement(224..649)
                     /locus_tag="VIN75_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974210.1"
                     /translation="MNKREFVAGGMAAMVAAPALAQAGGAPAHAPLQGLLARTRRLPD
                     LVERAGADAFQAYVGEQFDIAAGPDSGRRLAVAAVERVARCRSTEQFNVAFTRVGPAA
                     RGESGVEGVRVLVHGTGQRLSLHLEPDAAGYTARFNLLA"
     gene            complement(636..1166)
                     /locus_tag="VIN75_08330"
     CDS             complement(636..1166)
                     /locus_tag="VIN75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974211.1"
                     /translation="MTAVDAFTLRDATPSDRAFLLALYTSTRADEFAQFGWPPDMERA
                     FMQLQFEAQRGDYERRHPRARCRIVELHACPIGRLWTARERDRIVVLDITIVGDLRGQ
                     GIGSECLRGVQRQAANAGLAVELQVVVGNPAQHLYERLGFRQVGESGVRQEMSWRLEP
                     AAAPAPHSEEIHHEQA"
     gene            complement(1181..1711)
                     /locus_tag="VIN75_08335"
     CDS             complement(1181..1711)
                     /locus_tag="VIN75_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY8974212.1"
                     /translation="MADQFVGEVRMFCGNFAPAGWALCNGQLLPISQNTALFSLLGTN
                     YGGDGRSTFALPNLQGAFALHAGASAGPGLSARVTGQAGGAETVVLTTSQLPAHGHGM
                     AAVSGATTGTPDAAVSLAPTSNGSPLYRVPDNVWLNTAPGDIGASGGGAAHNNLPPYL
                     AVTFIIALQGIFPSRA"
     gene            complement(1724..2227)
                     /locus_tag="VIN75_08340"
     CDS             complement(1724..2227)
                     /locus_tag="VIN75_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY8974213.1"
                     /translation="MSEPYVGEIRLFAGNFAPQGWAFCDGQLLSIAQNEVLFDLIGTI
                     YGGDGVSTFQLPDLRGRVPVHQGNGYVQGQQGGQETVTLALAQMPAHRHAMNASTSPA
                     SATQGPSQVPGSSAAMRLYGSGAPNMTMASDALAPAGAGQPHENMPPFAALNYIISLF
                     GIFPSQS"
     gene            complement(2237..2746)
                     /locus_tag="VIN75_08345"
     CDS             complement(2237..2746)
                     /locus_tag="VIN75_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY8974214.1"
                     /translation="MSTPYLAEIRLTSFTFPPKGWAFCDGQLLAINQNQALFAVLGTM
                     YGGDGRTNFALPDLRGRAAMHASATHPAGEAGGEEAHTLTTGEMPAHGHALRASGKVA
                     ASSSPNGGVLAAVPRGGTPAYAAAGNLVPLNPAAIAASGGSQPHENRPPYLTLNFIIA
                     LQGIFPSRN"
     gene            3247..3702
                     /locus_tag="VIN75_08350"
     CDS             3247..3702
                     /locus_tag="VIN75_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974215.1"
                     /translation="MKLSCIPQGLVVAAALALASSGVFATELRALSETEMSAVYGQGL
                     SVPALNALGALSTKDQGNTALSASASDAQAGLAALASDAQNLDRQLQQQRLQNAAQGF
                     QTTLKITQTMLAVDKALAPIPINMGLPMMGMPFMFPLSLAGLDAIPGKH"
     gene            complement(3758..>5534)
                     /locus_tag="VIN75_08355"
     CDS             complement(3758..>5534)
                     /locus_tag="VIN75_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY8974216.1"
                     /translation="HEAAHRHHGAVTMASPRTLAALGALFALAACGGGGGGTSAPPPP
                     PAASTDAVLSVSLAGGATPGIDHLWVTVTGAAMHADATRVYGDGDPGWVVMTLPTPVT
                     LDLSDPALANGASVSLIRQSVSTLGTYAQLRLLLAPSDPSLAPLASATAKGLLFNEQV
                     QYTDANGAHVVPLEVPDAQGGLRVLMPFTLSADTTTPLSIEWSPEHSLVRRAATGGGA
                     DRFTLRNELQGYNQTLLTALSDGNLQIGGSIFDSISGQLDTTQFCTGASHTGCIHDVV
                     ATATSLSADKRFFAEARTVSVAADGSFELYPLPSQFAYDVVIRGGNMETIVVHGVVVD
                     PTGILKPAPTALSSKATPIVPVLDSGERAVTVTNALAPTSSRVFFGQTIAGTGGAGAD
                     VPYEIVSGAADPATGRLLYPVTLPGGPLHYASFNSTTDGNGTPPAFTTVTPVEGAGAW
                     STWSQGTLATTTSATAPLSSGATTVAAPQPVAAAGFTTGTLTVTLAGTASSGADHAEL
                     IVANDGGVVSVVDVSSQIASHGGSTTINVPSGSSSSAPGAAIYAVSVRTWTGSTEGAS
                     AHWSRTGAPVDLSTSATANVNLVLP"
BASE COUNT          760 a         1930 c         2062 g          782 t
ORIGIN      
        1 gtgctgccca gcccggcctg gatcgccggc tgcgtgctgt tcagcctcgt cggcttggcg
       61 gcctggcgct acggcaaggc cgtcgagcga cccgcgatcc gctggctcgg cctggcgctg
      121 atgctgtact cgtacttcac cgcgccgacg tggctgctct acggcgtggg cctcgcgctg
      181 tgcgcagcga tctggtggaa ccgccccagg gcggattgag ggctcaggcc agcaggttga
      241 agcgggccgt gtagcccgcg gcatcgggct ccaggtgcag cgacaggcgc tgtcccgtcc
      301 cgtgcaccag cacgcgcacg ccctcgacgc cggactcgcc ccgggccgcc ggccccacgc
      361 gcgtgaacgc cacgttgaac tgctccgtcg accggcagcg ggcgacgcgc tcgaccgcgg
      421 cgacggccag ccgccttccg gagtccgggc cggcggcgat gtcgaactgc tcgccgacgt
      481 aggcctggaa ggcgtcggcg ccggcgcgtt cgaccaggtc gggcaggcgg cgcgtgcgcg
      541 ccagcaggcc ttgcagcggc gcgtgcgccg gtgcgcctcc ggcctgcgcg agcgccggcg
      601 ccgcgaccat cgccgccatg ccgcccgcca cgaattcacg cttgttcatg atggatctcc
      661 tcggaatggg gcgccggcgc cgcggcgggc tcgaggcgcc aggacatctc ctggcggacg
      721 ccggattcgc cgacctggcg gaagcccagg cgctcgtaaa gatgctgcgc gggattgccg
      781 acgacaacct gcagctcgac ggccagcccg gcgttcgcgg cctggcgctg cacgccgcgc
      841 aggcactcgg agccgatgcc ctggccgcgc aggtcgccga cgatcgtgat gtcgagcacg
      901 acgatgcggt cgcgctcgcg cgccgtccac aggcggccga tcgggcaggc atgcagctcg
      961 acgatgcggc agcgcgcacg gggatgacgc cgctcgtagt cgccgcgctg ggcctcgaac
     1021 tgcagttgca tgaaggcgcg ctccatgtcc ggcggccagc cgaactgcgc gaactcgtcg
     1081 gcgcgcgtgc tggtgtacag cgcgagcagg aacgcgcggt cggaaggcgt cgcgtcacgc
     1141 agggtgaagg cgtcgacggc agtcatggcg gggcgtgccc tcaggcgcgc gaggggaaga
     1201 tgccttgcag ggcgatgatg aaggtcacgg ccaggtacgg cggcaggttg ttgtgcgccg
     1261 cgccgccgcc gctggcgccg atgtcgccgg gcgccgtgtt gagccagacg ttgtccggca
     1321 cgcggtacag cgggctgccg ttggaggtcg gcgccaggct caccgcggcg tcgggcgtgc
     1381 cggtggtggc gccggacacc gccgccatgc cgtggccgtg cgcgggcaac tgcgacgtcg
     1441 tgagcacgac cgtctcagcg ccgccggcct ggccggtgac tcgcgcgctc aggcccggtc
     1501 cggcgctggc cccggcatgc agcgcgaacg cgccctgcag gttgggcaat gcgaaggtcg
     1561 aacgcccgtc gccgccgtag ttggtgccca gcaatgagaa gagggcggtg ttctgcgaga
     1621 tcggcagcag ctggccgttg cagagcgccc acccggcggg cgcgaagttg ccgcagaaca
     1681 tccgcacctc gcccacgaat tgatcggcca tggggcagtc ctttcagctt tgcgagggga
     1741 agatgccgaa cagcgagatg atgtagttca gcgcggcgaa cggcggcatg ttctcgtgcg
     1801 gctggcccgc gcccgcaggc gcgagggcgt cggaggccat cgtcatgttc ggcgcgccgc
     1861 tgccgtacag gcgcatggcc gcggagctgc ccggcacctg cgacggtcct tgcgtggcgc
     1921 tggccggcga cgtgctcgcg ttcatcgcgt ggcggtgcgc cggcatctgg gccagcgcga
     1981 gcgtcaccgt ctcctggccg ccctgctggc cctgcacgta gccgttgccc tggtgcaccg
     2041 gcacgcgccc gcgcaagtcg ggcagctgga acgtggacac gccgtcgccg ccgtagatcg
     2101 tgccgatcag gtcgaacaac acctcgttct gcgcgatgga caggagctgc ccgtcgcaga
     2161 aggcccagcc ttgcggcgcg aaattgcctg cgaacaggcg gatctctccg acataaggtt
     2221 cagacatggg acctcgctag ttgcgggacg ggaagatgcc ttgcagcgcg atgatgaagt
     2281 tcagcgtgag atagggcggc cggttctcgt gcggctggct gccgccgctc gcggcgatgg
     2341 ccgcggggtt cagcggcacc aggttgccgg cggcggcata ggccggcgtc ccgccgcgcg
     2401 gcaccgcggc cagcacgccg ccgttgggcg acgaggacgc ggccaccttg cccgacgcgc
     2461 gcagcgcgtg tccgtgcgcg ggcatctcgc cggtggtgag cgtgtgcgcc tcctcgccgc
     2521 cggcctcgcc ggccgggtgc gtcgccgagg catgcatcgc cgcgcgcccc cgcaggtcgg
     2581 gcaacgcgaa attggtgcgg ccgtcgccgc cgtacatcgt gcccaggacc gcgaacagcg
     2641 cctggttctg gttgatcgcg agcaactggc cgtcgcagaa cgcccagccc ttgggcggga
     2701 acgtgaagct cgtgaggcgg atctcggcga ggtacggcgt cgacatggcg atcctttgcg
     2761 ggcaggacgg ggccggctgg ctgaggcgga tcacagcacc ggccacgggg cgtcgccatc
     2821 cccaggatgg aacttggggc gaacgctgcg gctttcagcg cgagctgaag gtgacgccgg
     2881 cccctgcgtc gcatgctcga ctgcgcggcc ggtcgcgcgc tcaccagcgt gtcccactca
     2941 agcgatggtc aggacggtcg atcgcgcgcg cgaggacgat tcgcttcttg gtatataccg
     3001 ggtgcggtat gcgatgcacc cgtccatgca tcacgattgc acatcgcctc gacgccagcg
     3061 gatgtgcacg gtcgttcgat cgatgcaggc gcgcgccgtc gccggtcagc gccggacgtg
     3121 aagtagttct cgcagtcgga tcgacggccg ctcatccgcc cggcgcagct gtcgatatgc
     3181 agggcatgcg ccgcagcgga ccgccaggtc gccgccgcgg ccgccccgca accgctggag
     3241 acgcgaatga agttgagctg catcccccaa ggcctcgtcg tggccgccgc cctcgcgctg
     3301 gcctcgtccg gggtgttcgc gaccgagctg cgcgcgctga gcgagacgga gatgtcggcg
     3361 gtctacggcc agggcctgag cgtcccggcg ctgaacgcgc tcggcgcgct gtcgacgaag
     3421 gaccagggca acaccgcgct gagcgcgtcc gcgagcgacg cgcaggccgg cctcgcggcg
     3481 ctcgcgagcg acgcccagaa cctcgaccgc cagctgcagc agcaacgcct gcagaacgcg
     3541 gcgcagggct tccagaccac gctcaagatc acgcagacga tgctcgcggt cgacaaggcg
     3601 ctggccccga tcccgatcaa catgggactg ccgatgatgg gcatgccgtt catgttcccg
     3661 ctgtcgctgg ccggcctgga cgcgatcccc ggcaagcact gagccacgcg gccgcaaagg
     3721 tcctcgggcc gcgccgcgga cccgaggcaa gcgccattca ggggaggacg agattgacgt
     3781 tggcggtggc cgacgtcgac aggtccaccg gcgcgcccgt gcgcgaccag tgtgcgctcg
     3841 cgccttcggt gctgccggtc cacgtgcgca ccgacaccgc gtagattgcc gcaccaggcg
     3901 ccgacgacga cgagccgctg ggcacgttga tcgtcgtgct gcccccgtgc gacgcgatct
     3961 gcgacgacac gtcgaccacg ctcaccacgc cgccgtcgtt ggcgacgatc agctccgcat
     4021 ggtccgcgcc gctgctggcg gtgcctgcca gcgtgaccgt cagcgtgcct gtcgtgaagc
     4081 ccgcggccgc gaccggctgc ggcgcagcca ccgtcgtcgc gccggagctc agcggcgccg
     4141 tcgcgctggt cgtcgtggcc agcgtgccct gcgaccatgt cgaccacgcg ccggcgcctt
     4201 ccaccggcgt caccgtcgtg aatgccggcg gcgtgccgtt gccgtcggtc gtgctgttga
     4261 agctcgcgta atgcaacggg ccgcccggca gcgtgaccgg gtacagcagc cggccggtcg
     4321 ccggatcggc ggcgcccgac acgatctcgt acgggacatc ggcgccggcg ccgccggtgc
     4381 cggcgatcgt ctggccgaag aacacgcggc tggacgtcgg cgccagcgcg ttcgtgacgg
     4441 tgacagcgcg ctcgccgcta tcgagcaccg gcacgatggg cgtggccttg ctgctgagcg
     4501 cggtgggtgc gggcttgagg atgccggtgg gatcgacgac cacgccatgc accacgatcg
     4561 tctccatgtt gccgccgcgg atgacgacgt cgtaggcgaa ctggctgggc agcgggtaca
     4621 gctcgaagct gccgtcggcc gccacgctga cggtgcgcgc ctccgcgaag aagcgcttgt
     4681 cggccgaaag gctggtggcc gtggccacga cgtcgtggat gcagcccgtg tggctcgcgc
     4741 cggtgcagaa ctgcgtggtg tcgagctggc cggagatcga gtcgaagatg ctgccgccga
     4801 tctgcaggtt gccgtcgctc agcgccgtca gcagcgtctg gttgtagccc tgcagctcgt
     4861 tgcgcagcgt gaagcggtcg gcgccgccgc cggtggccgc gcggcgcacc agcgagtgtt
     4921 cggggctcca ctcgatcgac aacggcgtcg tcgtgtcggc gctgagggtg aacggcatga
     4981 gcacgcgcag gccgccctgc gcgtcgggca cctccagcgg cacgacgtgc gcgccgttcg
     5041 cgtcggtgta ctgcacctgc tcgttgaaca gcaggccctt ggcggtggcc gaggccagcg
     5101 gggccagcga cggatcggac ggcgccagca gcaggcgcag ctgcgcgtag gtgcccagcg
     5161 tggacacgct ctgcctgatc agcgagaccg aggcgccgtt ggccagcgcc ggatcggaca
     5221 ggtcgagcgt gaccggcgtg ggcagcgtca tcacgaccca gccggggtcg ccgtcgccgt
     5281 acacgcgcgt cgcgtcggca tgcatcgccg cgccggtgac cgtgacccac aggtggtcga
     5341 tgcccggcgt ggcgccaccg gccagcgaga cgctcaggac cgcgtccgtc gaggcggccg
     5401 gcggcggcgg gggcgcgctg gtgccgccgc ctccgccgcc gcacgcggcg agcgcgaaca
     5461 gggcgccgag cgcggcgagc gtgcggggcg aggccatggt cactgcgccg tggtggcgat
     5521 gcgcagcttc gtgc
//
LOCUS       DATCRO010000307         8116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_903201, whole genome shotgun sequence.
ACCESSION   DATCRO010000307 DATCRO010000000
VERSION     DATCRO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903201"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VIN75_08360"
     CDS             complement(<1..1779)
                     /locus_tag="VIN75_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY8974217.1"
                     /translation="MPENKTTKPMSHVLVVSADPGWREEVVAGINAAAPKLDNPFDLL
                     AIAAADAAAAVAAVLADGDIQIVVVDHGEHAPAGKAGKGAARHQDVAAMAQEIIALRP
                     ELSLYVTLVDDENKLLLEQLASDSVHGYFYRGEHDYAGWARILAAEIAEKSATPFYDE
                     LKNYVRMAKDSWHTPGHAGGDSLKGSPWVGDFYDFAGENLLRADLSVSVEMLDSLLHP
                     TGVIVQAQRLAAQAFGARKTYFATNGTSTANKVIFQTLLAPGDKMLLDRNCHKSVHHG
                     VILSGAQPVYLDSSVNRKYGLFGPVPRATVLAAIEQHPDARVLILTSCTYDGLRYDLK
                     PIIDAAHAAGIKVIVDEAWYGHARFHPALRPTALESGADYCTQSTHKVLSAFSQASMI
                     HVNDPLFDEHLFRENFNMHASTSPQYNLIASLDVARKQSVMEGYRLLDRSLKYAQELR
                     EKINATGVFRVLDLADLLPEELAGDGIRLDPTKITVDITASGFSSDELRAELFERFNI
                     QVEKSTHNTLTLLLTVGTTRSKVSRLFDALLRLSKERRPERASARMPELPHFTRLACL
                     PRDAFYEYGERIPLLDDQGQPNKALDG"
     gene            2342..4165
                     /locus_tag="VIN75_08365"
     CDS             2342..4165
                     /locus_tag="VIN75_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8974218.1"
                     /translation="MSFDTQSPLGNEDQQDALPEEAATGGFDTLGLAPEIVRSVKEAG
                     YTDPTEVQAQAIPAAISGVDLLVSSRTGSGKTAAFVLPALQRIIEARRDPAKKRERGQ
                     PSGPRVLVLAPTRELAMQVSKACSVYGQMVQGLRVATIVGGVPYHAQLKALRGPLDIL
                     IATPGRLIDHMGSGKAMMSQVELLVLDEADRMLDMGFIDDIHHVAEQLSKERQTMMFS
                     ATFEGNVGNLAKELTHEAQRIRVSAPTEQHSQIEQRLHWADNRKHRDDLLDQLLTDRD
                     MDQAVVFTSTQRDADELADRLQQIGHSVAALHGGMPQGRRNRVLGALRSRQLRVLIAT
                     DVAARGIDVPTITHVINYGLPMKAEDHVHRIGRTGRAGRSGLAITLAERRDLGMIRRI
                     ERFTGNNLPVAIVPGLEPRLPAPAPARAGGPRRFDDERPHGGHRSHFNNGPRGGFGGH
                     RGGDRDGFGQRPGFGGEQRPRFGHGGDRPQHPGERPHFGERPQQHGDRRPFGDRPQFQ
                     REGGEQRPFRTDNPFQRNDGPRGDYRGQRPQQGDRPAFNRDGGDRPAFNREGGERAHA
                     RPHGDRPSFGDRPQREGYGERKGFGAKQGGNGPRDPRFRGR"
     gene            complement(4464..5552)
                     /gene="rsgA"
                     /locus_tag="VIN75_08370"
     CDS             complement(4464..5552)
                     /gene="rsgA"
                     /locus_tag="VIN75_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8974219.1"
                     /translation="MHDSFDLRALRDIGLSHDTVQAALARPRPDGTRLARVIEIQRDH
                     LVAHDGTDAHAVQPTPALRAALEHEHDTLVVGDWVWWRAAASDDDRGWVVARLPARNR
                     VSRRDPAGGRQGLVANIDTALIVMGLDHDFNLRRLDRFLVLAHSADARVVVVLTKADA
                     CGDPAGAIARVRAHLGDARDDVAVLAVDGRSHDTVAALAPWLVRGATLVTLGSSGAGK
                     TTLTNTLAGAAALTGAVRATDERGQHTTTVRTLHRTDGGACLIDTPGLRQLWLDANAD
                     TLNDAFPEIGMLALRCRYRNCRHAQEPGCAVRELVPRERLVSFQKLLREANRESDDIF
                     QRRRHVAVMKQRTRENRARNAGRANGEW"
     gene            5974..6438
                     /locus_tag="VIN75_08375"
     CDS             5974..6438
                     /locus_tag="VIN75_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8974220.1"
                     /translation="MRPALLACALSVCAFDALAAAPLPSADDLAAIAAEVRASEIAFA
                     RTMADRKLDRFTDFVAEDAVFNGRSAQIGRTAVIDAWKRFFEGAQAPFSWGPDAVAPT
                     ADGRFAISTGLVHNPEGKVTGRFTSIWRKDADGHWRVVADQGVDAGCAEEKK"
     gene            complement(6644..>8116)
                     /locus_tag="VIN75_08380"
     CDS             complement(6644..>8116)
                     /locus_tag="VIN75_08380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8974221.1"
                     /translation="PFVRASARQAAIDRGMAEGMHVVTLSRSLIVPFLTFSNRRELRE
                     RAWKAWVGRGEIEGETDNHEVARDILRLRQEQAWMHGHSSYAEYALANTMAGNVASVN
                     TLLDDVYSRALTALQDERKAVHEALRDAGVPAPYMAWDWRWGSEKVRQQRYAFDDGEV
                     KPYFSLERMVQAAFDCAQRLFGIRMVRNDAIPVYHPDVVPYEVHDGDRLIGIFLHDNF
                     ARQTKRSGAWMSALRWQCRNGGVDLPVIMNNNNFAKGAPGEPTLLSFDDARTLFHEFG
                     HGLHGLLSNVTYQRLSGTNVLRDFVELPSQLFEHWLLEPEVIARHARHWQTGEPIPET
                     LVRRLQEARRWGQAYETVRYAGSALVDMAVHSRQDKEPPPDLPEFEAATLARLNLPPE
                     VGINHRLLHFQHLFSGSAYAAGYYVYLWAEVLDADAFDAFKEAGNAFDPATAARLRQC
                     IYGAGDSVAPQQAYASFRGRMPSIEPLLRKRGLLPEDVEY"
BASE COUNT         1189 a         2868 c         2859 g         1200 t
ORIGIN      
        1 gccgtcgagg gccttgttcg gctggccctg gtcgtccagc agcgggatgc gctcgccgta
       61 ctcgtagaac gcgtcgcgcg gcaggcaggc gaggcgcgtg aagtgcggca gctcgggcat
      121 gcgggcgctc gcgcgctcgg gacgacgctc cttggacagg cgcagcagcg cgtcgaacag
      181 gcgcgacacc ttcgagcgtg tcgtgcccac cgtcagcagc agcgtgagcg tgttgtgcgt
      241 cgacttctcg acctggatgt tgaagcgctc gaacagctcg gcgcgcagct cgtccgacga
      301 gaagcccgag gcggtgatgt ccaccgtgat cttcgtcggg tcgaggcgga tgccgtcgcc
      361 ggcgagttcc tcgggcagca ggtcggccag atccagcacg cggaacacgc cggtcgcgtt
      421 gatcttctcg cgcagctcct gcgcgtactt cagcgagcgg tcgagcagcc ggtagccttc
      481 catcaccgac tgcttgcgcg ccacgtccag gctcgcgatc aggttgtact gcgggctggt
      541 cgacgcgtgc atgttgaagt tctcgcggaa caggtgctcg tcgaacagcg ggtcgttgac
      601 gtggatcatg ctggcctgcg agaaggccga cagcaccttg tgcgtgctct gcgtgcagta
      661 gtcggcgccg ctctccagcg cggtggggcg cagcgccgga tggaagcgcg cgtggccgta
      721 ccaggcctcg tcgacgatca ccttgatgcc cgcggcgtgc gcggcgtcga tgatgggctt
      781 gaggtcgtag cgcaggccgt cgtaggtgca ggacgtcagg atcagcacgc gcgcgtccgg
      841 atgctgctcg atggcggcca gcacggtggc gcgcggcacc gggccgaaca ggccgtactt
      901 gcggttgacc gacgagtcca ggtacaccgg ctgcgcgccc gacaggatca cgccatggtg
      961 gaccgacttg tggcagttgc gatccagcag catcttgtcg ccgggcgcca gcagcgtctg
     1021 gaagatcacc ttgttggcgg tggacgtgcc gttggtggcg aagtaggtct tgcgcgcgcc
     1081 gaaggcctgc gcggccaggc gctgcgcctg cacgatgacg ccggtcgggt gcagcagcga
     1141 gtcgagcatc tccaccgaca ccgacaggtc ggcgcgcagc aggttctcgc cggcgaagtc
     1201 gtagaagtcg ccgacccacg ggctgccctt gagggagtcg ccgcctgcgt ggcccggcgt
     1261 gtgccagctg tccttggcca tgcgcacgta gttcttgagc tcgtcgtaga acggcgtggc
     1321 ggacttctcg gcgatctcgg cggccaggat gcgcgcccag cccgcgtagt cgtgctcgcc
     1381 gcgatagaag tagccgtgca cggaatcgct ggccagctgt tccagcagca gcttgttctc
     1441 gtcgtccacc agcgtgacgt acagcgacag ctccggccgc agcgcgatga tctcctgcgc
     1501 catcgccgcg acatcctggt ggcgcgccgc gccctttccg gccttgcccg cgggcgcatg
     1561 ctcgccgtgg tcgaccacga cgatctggat gtcgccgtcg gccagcaccg cggccacggc
     1621 cgcggccgca tcggcggcgg cgatcgccag caggtcgaac gggttgtcga gcttcggcgc
     1681 ggccgcgttg atgccggcga cgacctcctc tcgccagccc gggtcggcgc tcaccaccag
     1741 gacgtggctc atcggtttgg tcgtcttgtt ctcgggcatg ggtgtctcgc ggtggatggg
     1801 cgcggccctc gtggggcggc ccggtcgttg tcgtgtctgc gggcgtcccc ggaggtcgac
     1861 ttgggacgcg tgaagcacgt cacggaacgt gctgcgcagc aaacccgtca tggtagacca
     1921 gtgcgcgaag tggaaaattc tggcaaaata ccagttgacg ctgcgaatgc actctcagga
     1981 acgcccgacc gtcataggca aggcaccaaa agggcgcgca cgtcttcgct catcccctct
     2041 gaccctttca agccggtctg ctgccgatat ccgacaaacc tcagggttat ccctggaggc
     2101 ctgctcggtg aacggcactc ggaaacggtt gtggtcggcg gcgatgccgt cgctaccaga
     2161 cgtttcctgg gcattcaccc gccgctcgcc acctggcgca cgcgacggcc cgccccgtta
     2221 tcttctttac gcgacctgtt tggcgctgcc gagacctcac ccggcaggcg gcgccgtggc
     2281 gcatcctcgt gcgtggccga ccggccaggc gcgcgggctg cgtcgtgctt gaaagtgact
     2341 gatgagtttc gatacgcaat ccccgctggg caacgaagac caacaagacg ccctgcctga
     2401 agaagccgcc acgggcggct tcgacaccct gggcctcgcg cccgagatcg tccgctccgt
     2461 caaggaagcg ggctacaccg acccgaccga agtccaggcg caagccatcc ccgccgccat
     2521 ctccggcgtc gacctgctgg tgtcctcgcg cacgggcagc ggcaagaccg ccgccttcgt
     2581 gctgccggcg ctgcagcgca tcatcgaggc gcgccgcgac cccgccaaga agcgcgagcg
     2641 cggccagccg agcggcccgc gcgtgctggt gctcgccccc acgcgcgaat tggcgatgca
     2701 ggtcagcaag gcctgttccg tctatggcca gatggtgcag ggcctgcgcg tggccaccat
     2761 cgtcggcggc gtgccgtacc acgcccagct caaggccctg cgcggcccgc tggacatcct
     2821 gatcgccacc cccggccgcc tgatcgacca catgggcagc ggcaaggcca tgatgtcgca
     2881 ggtcgagctg ctggtgctgg acgaggccga ccgcatgctg gacatgggct tcatcgacga
     2941 catccaccac gtcgccgagc agctgtccaa ggaacgccag acgatgatgt tcagcgcgac
     3001 gttcgaaggc aacgtcggca acctcgccaa ggaactgacg cacgaagcgc agcgcatccg
     3061 cgtctcggcg cccaccgagc agcactcgca gatcgagcag cgcctgcact gggccgacaa
     3121 ccgcaagcat cgcgacgacc tgctggacca gctgctgacc gaccgcgaca tggaccaggc
     3181 cgtcgtcttc acgagcaccc agcgtgacgc cgacgagctc gccgaccgcc tgcagcagat
     3241 cggccactcg gtcgccgcgc tgcacggcgg catgccgcaa ggccgccgca accgcgtcct
     3301 gggcgcgctg cgcagccgcc agctgcgcgt gctgatcgcc accgacgtcg ccgcgcgcgg
     3361 catcgacgtg cccacgatca cgcacgtcat caactacggc ctgccgatga aggccgaaga
     3421 ccacgtgcac cgcatcggcc gcaccggccg tgccggccgc tcgggcctgg cgatcacgct
     3481 ggccgagcgc cgcgacctgg gcatgatccg ccgcatcgag cgcttcacgg gcaacaacct
     3541 gcccgtggcg atcgtcccgg gcctcgagcc gcgcctgccc gcgcccgccc cggcgcgcgc
     3601 cggcggcccg cgccgcttcg acgacgagcg cccgcacggc ggccaccgca gccacttcaa
     3661 caacggcccg cgcggcggct tcggcggcca tcgcggcggc gaccgcgatg gcttcggcca
     3721 gcgccccggc ttcggcggcg agcagcgccc gcgcttcggc cacggcggcg atcgtccgca
     3781 gcaccctggc gagcgcccgc acttcggcga gcgtccgcag cagcacggcg accgccgccc
     3841 gttcggcgac cgtccgcagt tccagcgcga gggcggcgag cagcgcccgt tccgcacgga
     3901 caacccgttc cagcgcaacg acggcccccg cggcgactac cgtgggcagc gtccgcagca
     3961 gggcgatcgt ccggcgttca atcgcgacgg cggcgatcgc ccggcattca accgcgaagg
     4021 cggcgagcgc gcgcacgcgc gtccgcatgg cgaccggccg tcgttcggtg atcgtcccca
     4081 gcgcgaaggc tacggcgagc gcaagggctt cggcgccaag cagggcggca acggcccgcg
     4141 cgacccgcgc ttccgcggcc gctgagcgtc gggcgcctcg gcgctgacgt gagccgaccg
     4201 agaaggccgc cccgcaacgg gcggcctctt ttttttttca tggctccgag gggtctggcc
     4261 tctccttcgt tcgcggcgct ctgctgatcg aagccgcttg atcgaatcgc ggacgaaggc
     4321 gaaggcctga ccccgatcgt gtgcagatga tcgaggtcag gccttcgcct cgaatcgcgg
     4381 ccagcgcatc gacgggtgga gacagcgagc gcgatccgag gagaggccag cccccccggc
     4441 aacggcgtgc caccgccatg ggatcaccac tccccgttgg cccgccccgc attgcgtgcc
     4501 cggttctccc gcgtccgctg cttcatcacg gccacatgcc gccgccgctg gaagatgtcg
     4561 tcgctctcgc gattggcctc gcgcagcagc ttctggaagc tcacgaggcg ctcgcgcggc
     4621 accagttcgc gcaccgcgca gccaggctcc tgcgcgtggc ggcagttgcg gtagcggcag
     4681 cggagcgcca gcatgccgat ctcggggaag gcgtcgttga gcgtgtcggc gttggcgtcg
     4741 agccacagct ggcgcaggcc cggcgtgtcg atcaggcagg cgccgccatc ggtgcgatgc
     4801 agcgtgcgca ccgtcgtcgt gtgctggccg cgttcgtcgg tggcacgcac cgcgccggtg
     4861 agcgcggcgg cgcccgccag cgtgttggtg agcgtcgtct tgcccgcgcc gctggagccc
     4921 agcgtcacca gcgtcgcgcc gcgcacgagc cagggcgcga gcgcggccac ggtgtcgtgc
     4981 gagcggccgt cgacggccag caccgcgacg tcgtcgcgcg cgtcgcccag gtgcgcgcgc
     5041 acgcgcgcga tcgcgcccgc agggtcgccg cacgcgtcgg ccttggtgag cacgaccacc
     5101 acgcgcgcgt cggcggagtg ggcgagcacg aggaagcggt cgaggcggcg caggttgaag
     5161 tcgtggtcga ggcccatgac gatgagcgcg gtgtcgatgt tggccaccag cccctggcgg
     5221 ccgccggccg ggtcgcggcg cgacacgcgg ttgcgcgcgg gcaagcgcgc gacgacccag
     5281 ccgcggtcgt cgtccgacgc ggccgcgcgc caccacaccc agtcgccgac gaccagggtg
     5341 tcgtgctcgt gctccagcgc cgcgcgcagg gccggcgtcg gctgcaccgc gtgcgcgtcg
     5401 gtgccgtcgt gcgcaacgag gtggtcgcgc tggatctcga tgacgcgcgc cagccgcgtg
     5461 ccatccggtc gcggtcgcgc gagcgcggcc tgcaccgtgt catgggaaag tccgatgtcg
     5521 cgcagcgcgc gcagatcgaa ggaatcgtgc atttggaaag gttccggacg cgccgcatcg
     5581 cgtgttcggc gatgcggcag cttcgctcga gctcggcgtg agcgcgaacg aacgaaggcc
     5641 ttgcacggtc aaggccggcg atgggagtgt gggcgtcgga accggctggc gtcggtgcgg
     5701 gcaggtgcgc tctgcttgga gcggggcctc gcaccggagg ctgcggcgtt cagtcggaag
     5761 gactgtctgc gggagcctgc gattaccgtg acgacaaggc ggtcgacgca ctggcgaatt
     5821 gggtatccat ggcgacctcc tgaaggcgag acggttgaag ggagccgcga ctttgcgccc
     5881 gcatgcgcgc catgtcaaga ccgcgcgccc gcatggcggc gtgcgcgcca cacgtccaca
     5941 atcgccgcac ctcgactgcc ggagcgatcc gccatgcgcc ctgccctgct cgcctgcgcc
     6001 ctgtccgtct gcgcgttcga cgcgctcgcc gccgcgccgc taccgtcggc cgacgacctg
     6061 gcggccatcg ccgccgaggt cagggccagc gagatcgcgt tcgccaggac gatggccgac
     6121 cgcaagctcg accggttcac cgacttcgtc gccgaggacg cggtgttcaa cggccgcagc
     6181 gcgcagatcg gccgcaccgc cgtgatcgac gcctggaagc gcttcttcga gggggcgcag
     6241 gcgccgttca gctggggccc tgacgccgtg gccccgaccg ccgacggtcg cttcgcgatc
     6301 tcgacgggcc tggtgcacaa ccccgaaggc aaggtcaccg ggcgcttcac cagcatctgg
     6361 cgcaaggatg cggacggcca ctggcgcgtg gtggccgacc agggcgtgga cgcgggctgc
     6421 gccgaagaaa agaagtagcg cccgaaggtg ccggaggggt ctggcctctc ctcgaatcgc
     6481 gtggcaacgt ccgtcgcgag ccgtgatgca ggccgcgatt cgaggccaag gcctgacccc
     6541 gatcatcggc tgacgttcgg ggtcaggcct tcgcctcaag tcgccggttg ccgtatcgcc
     6601 gatggcgacg aaatggcggc gacgtgagga gaggccagac ccctcagtac tcgacgtcct
     6661 ccggcagcag cccgcgcttg cgcagcagcg gctcgatcga cggcatgcgc ccgcggaacg
     6721 acgcgtaggc ctgctgcggc gccacgctgt cgccggcccc gtagatgcac tggcgcaggc
     6781 gcgccgcggt ggccggatcg aacgcgttgc ccgcctcctt gaacgcgtcg aacgcgtcgg
     6841 cgtccagcac ctcggcccac aggtagacgt aataccccgc ggcgtacgcc gagcccgaga
     6901 acaggtgctg gaagtgcagc aggcggtggt tgatgccgac ctcgggcgga aggttcaggc
     6961 gcgcgagcgt ggcggcctcg aactcgggca ggtccggcgg cggctccttg tcctggcgcg
     7021 aatgcacggc catgtccacc agcgcgctgc cggcgtagcg caccgtctcg taggcctggc
     7081 cccagcggcg cgcctcctgc aggcggcgca ccagcgtctc ggggatgggc tcgcccgtct
     7141 gccagtggcg cgcgtggcgc gcgatcacct cgggctccag cagccagtgc tcgaacagct
     7201 gcgacggcag ctcgacgaag tcgcgcagca cgttggtgcc cgacaggcgc tggtaggtca
     7261 cgttcgacag cagcccgtgc aggccgtggc cgaattcgtg gaacagcgtg cgcgcgtcgt
     7321 cgaacgacag cagcgtcggc tcgccgggtg cacccttggc gaagttgttg ttgttcatga
     7381 tgaccggcag gtcgacgccg ccgttgcggc actgccagcg cagcgcgctc atccacgcgc
     7441 ccgaccgctt ggtctggcgt gcgaagttgt cgtgcaggaa gatgccgatc agcctgtcgc
     7501 cgtcgtgcac ctcgtagggc acgacgtccg ggtggtacac ggggatcgcg tcgttgcgca
     7561 ccatgcggat gccgaacagc cgctgcgcgc agtcgaacgc ggcctgcacc atgcgctcga
     7621 gcgagaagta cggcttgacc tcgccgtcgt cgaacgcgta gcgctgctgg cgcaccttct
     7681 ccgagcccca gcgccagtcc caggccatgt acggcgccgg cacgccggca tcgcgcaagg
     7741 cctcgtgcac cgccttgcgc tcgtcctgca gcgcggtcag cgcgcgcgag tagacgtcgt
     7801 ccagcagcgt gttgaccgat gccacgttgc cggccatggt gttggcgagc gcgtattcgg
     7861 catagctcga atggccgtgc atccaggcct gctcctggcg cagccgcagg atgtcgcgcg
     7921 cgacctcgtg gttgtcggtc tcgccctcga tctcgccgcg gccgacccag gccttccatg
     7981 cgcgttcgcg cagctcgcgc cggttcgaga aggtcaggaa cggcacgatc agcgagcgcg
     8041 acagcgtcac cacgtgcatg ccttcggcca tgccgcggtc gatggcggcc tggcgcgcgg
     8101 acgcgcgcac gaacgg
//
LOCUS       DATCRO010000308         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_858345, whole genome shotgun sequence.
ACCESSION   DATCRO010000308 DATCRO010000000
VERSION     DATCRO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858345"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VIN75_08385"
     CDS             complement(<1..1008)
                     /locus_tag="VIN75_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8974222.1"
                     /translation="MPAVPAASSARPRRFAAAAAVAAFALVLVPVSANVPRKPAAAAP
                     AKAAAASAPPPATTTDLAQWCARLAPAVPGVDQPACRASGLRPGDGRSVHGVPLWTND
                     VLPATPPRFKVLLLGGIHGDELASVTLAFDWIARSRGVQASAPTRTPKAAKPSTSDVA
                     WRFVPLLNPDGLLHSPSTRVNAHGVDLNRNFPTADWARDAQPYWIKRTRRDPRRFPGL
                     QPLSEPESRWLIEQIDQFHPDLIVSVHAPYGLLDFDGPPPAPRRLGSLQLDPVGVYPG
                     SLGNYGGLVKNVPVMTLELKNARAVPPQELAAMWSDLQRWIREQVTNPGVQASAQRSP
                     VP"
     gene            1119..2594
                     /locus_tag="VIN75_08390"
     CDS             1119..2594
                     /locus_tag="VIN75_08390"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY8974223.1"
                     /translation="MIEPLLVPRESFDDPDAAVARVREIYQAGVDHLRRHLHDFVAGK
                     VPAAHVRACYPSVRVHTDTVARADSVSSYGFVAGPGSFETTLTRPELFAEYYRTQFEL
                     LRQHHGVKIEVGVSREPMPLHFSFAEHDHVEGTLTMAQRLQLRDLFDLPNLAAMDDGI
                     ANGTHEPGPNEPLPLALFTAPRVDYSLHRLRHYTGTAPDHFQNFVLFTNYQFYIDEFI
                     ALGHREMAREDSVYDAFVEPGNVVTMRAGLPAPVGPAGHAPPRLPQMPAYHLRRPDNS
                     GITMVNIGVGPSNAKNISDHIAVLRPHAWIMLGHCAGLRTTQQLGDYVLAHGYVREDH
                     VLDEELPLWVPIPPLAEVQVALEQAVADITQLQGLNLKRVMRTGTVASTDNRNWELLP
                     HPGPERRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLPGMANHF
                     YRERVEQHLRIGIRALEILRGAGVDKLHSRKLRGFAEVAFQ"
     gene            2769..>3050
                     /locus_tag="VIN75_08395"
     CDS             2769..>3050
                     /locus_tag="VIN75_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8974224.1"
                     /translation="MTILESSDTAAIRNVALVGASATGKTTLTEALLLRAGVLKQPGS
                     VERGSTVSDHDPLEKRVGHSLNTAVVNCVHDGIRIQLLDTPGALEFVGQS"
BASE COUNT          452 a         1123 c         1064 g          411 t
ORIGIN      
        1 cggcaccggg ctgcgctgcg cgctcgcctg cacgccgggg ttggtcacct gctcgcggat
       61 ccaccgctgc aggtcgctcc acatcgccgc cagttcctgc ggcggcacgg cgcgcgcgtt
      121 cttgagctcc aacgtcatca cgggcacgtt cttcaccagg ccgccgtagt tgcccagcga
      181 acccggatag acgcccaccg ggtccagctg caggctgccg aggcggcgcg gcgcgggcgg
      241 cgggccgtcg aaatcgagca ggccgtacgg cgcgtgcacc gacacgatga ggtcggggtg
      301 gaactggtcg atctgctcga tcagccagcg cgactcgggc tcggacagcg gctgcaggcc
      361 gggaaagcgc cgcgggtcgc gcctggtccg cttgatccag tacggctggg cgtcgcgcgc
      421 ccagtcggcg gtggggaagt tgcggttcag gtcgacgcca tgcgcgttga cgcgggtcga
      481 cggcgagtgc agcaggccgt cggggttgag cagcggcacg aaacgccagg cgacgtcgga
      541 ggtcgacggc ttggccgcct tcggtgtccg cgtcggcgcc gacgcctgca cgccgcgcga
      601 gcgggcgatc cagtcgaagg ccagcgtgac ggaggccagc tcgtcgccgt ggatgccgcc
      661 cagcagcagc accttgaacc tgggcggggt cgcgggcagc acgtcgttcg tccacagcgg
      721 cacgccgtgc accgagcggc cgtcgccggg ccgcaggccc gaggcgcggc acgcgggctg
      781 gtcgacgccg ggcaccgccg gcgcaagacg cgcgcaccac tgcgcgaggt cggtggtggt
      841 cgcgggcggc ggcgcggacg cggctgccgc cttcgccggc gcggcggccg cgggcttgcg
      901 cggcacgttg gccgatacgg gcaccagtac cagcgcgaac gcggcgacgg cggccgccgc
      961 cgcgaagcgg cgtgggcgcg ccgaagaggc cgccggaact gccggcaagc cagaaaaatc
     1021 gggggtctcg ctgttcttca ccccgacagt gtagaagtgc gcggaccggg ccctgaggcg
     1081 gatgcgccgc cgtcacgcct ttcatccaca atgaacccat gatcgaaccg ctgctcgtcc
     1141 cccgcgaatc cttcgacgat cccgacgccg ccgtggcgcg cgtgcgcgag atctaccagg
     1201 ccggcgtcga ccacctgcgc aggcacctgc acgacttcgt ggccggcaag gtgcccgccg
     1261 cgcacgtgcg cgcctgctat ccgagcgtgc gcgtgcacac cgacacggtg gcgcgcgccg
     1321 actcggtctc cagctacggc ttcgtcgccg gccccggcag cttcgagacg acgctgacgc
     1381 gccccgagct gttcgccgag tactaccgca cccagttcga gctgctgcgc cagcaccacg
     1441 gcgtgaagat cgaggtgggc gtcagccgcg agccgatgcc gctgcacttc agcttcgccg
     1501 agcacgacca cgtcgagggc acgctgacca tggcgcagcg cctgcagttg cgcgacctgt
     1561 tcgacctgcc caacctggcg gcgatggacg acggcatcgc caacggcacg cacgagccgg
     1621 gccccaacga gccgttgccg ctggccctgt tcacggcgcc gcgcgtcgac tactcgctgc
     1681 accgcctgcg ccactacacc ggcacggcgc ccgaccactt ccagaacttc gtgctgttca
     1741 cgaactacca gttctacatc gacgagttca tcgcgctggg tcaccgggag atggcccgcg
     1801 aggactcggt gtacgacgcg ttcgtcgagc cgggcaacgt cgtgacgatg cgcgccggcc
     1861 tgcccgcacc cgtgggcccg gccggccacg caccgccgcg cctgccgcaa atgcccgcgt
     1921 accacctgcg ccggccggac aacagcggca tcacgatggt caacatcggc gtcgggccgt
     1981 ccaacgccaa gaacatcagc gaccacatcg cggtgctgcg cccgcacgcc tggatcatgc
     2041 tgggccactg cgccggtctg cgcaccactc agcagctggg cgactacgtg ctcgcgcacg
     2101 gctacgtccg cgaggaccat gtgctcgacg aggagctgcc gctgtgggtg ccgatcccgc
     2161 cgctggccga ggtgcaggtg gcgctggagc aggcggtggc ggacatcacg cagctgcagg
     2221 gcctgaacct caagcgcgtg atgcgcaccg gcactgtcgc gtccaccgac aaccgcaact
     2281 gggagctgct gccgcacccg ggccccgagc gccgcttcag ccagagccgc gcgatcgcgc
     2341 tcgacatgga atcggctacc atcgccgcca acgggttccg cttccgcgtg ccctacggca
     2401 ccttgctgtg cgtgtcggac aagccgctgc acggcgagat caagctgccc ggcatggcca
     2461 accatttcta ccgcgagcgc gtcgagcagc acctgcgcat cggcatccgc gcgctcgaga
     2521 tcctgcgcgg cgcgggtgtg gacaagctgc acagccgcaa gctgcgcggg ttcgcggaag
     2581 tggccttcca ataggttgcc gccatcctgc ggctgcgcgc gggatgacga cgtcgccggg
     2641 tcgcggcgaa ggcggccatt gcgcgcccgc ctcgagcggc gtaggctggg ggttcctgcc
     2701 atccggccgt gacgcggttc gggtcgccgc ggtgccgcct ggcgtcacgg cccgtggaga
     2761 cacgcgccat gacgatcctg gagtcctccg ataccgcggc gattcgcaac gtggccctgg
     2821 tgggcgcctc ggccaccggg aagaccaccc tcaccgaggc cctgctgctg cgcgccggcg
     2881 tcctcaagca gcccggcagc gtcgaacgcg gctcgaccgt cagcgaccac gacccgctcg
     2941 agaagcgcgt cggccactcc ctcaacaccg ccgtcgtcaa ctgcgtgcac gacggcatcc
     3001 gcatccagct gctcgacacg cccggggcgc tcgagttcgt cgggcaatcg
//
LOCUS       DATCRO010000309         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_149738, whole genome shotgun sequence.
ACCESSION   DATCRO010000309 DATCRO010000000
VERSION     DATCRO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149738"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIN75_08400"
     CDS             <1..466
                     /locus_tag="VIN75_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY8974225.1"
                     /translation="HPAALQRAREIAKKVTDDLGGLGLFGVELFVKGEQVWFSEVSPR
                     PHDTGMVTMITQWQSEFELHARAILGLPVDVSLKSPGASAVIYGGVDAKAVRFDGVAD
                     AMRVPQTELRLFGKPESFERRRMGVALAHDTDVERARVKAKEAAAKVKPRAA"
     gene            complement(482..1090)
                     /gene="gstA"
                     /locus_tag="VIN75_08405"
     CDS             complement(482..1090)
                     /gene="gstA"
                     /locus_tag="VIN75_08405"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY8974226.1"
                     /translation="MELYYSPGACSLSPHIALREAGVKFDLVKTALQTHTLADGSDYY
                     QITAKGQVPLLELDDGARLSEGPVILQYIADHAPASGLAPAAGTMERYRVMEWLNFIT
                     SELHKGFSPMFNPKLAKDAKDVFAARLQERYRLVDARLEGNDYLMGKQFSVADAYLFT
                     VTNWAAGVGVDLAGLKNLEAFMARMKARPAVQDAMKAEGLVK"
     gene            1278..1658
                     /locus_tag="VIN75_08410"
     CDS             1278..1658
                     /locus_tag="VIN75_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY8974227.1"
                     /translation="MTRPILDVSRIHPAIRARIADEGRAVIAEVQAAIAANKVVVVGM
                     GWNPFPRKARKWLDAAGVPYQYIEYGNYASQWRKRLPLKLWTGWTTFPMVFVDGQLVG
                     GFRDLARLDPATLKKGAGAASAHP"
     gene            1655..>2801
                     /locus_tag="VIN75_08415"
     CDS             1655..>2801
                     /locus_tag="VIN75_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8974228.1"
                     /translation="MTRRFVRRAALAAALAFAGAGAWGATLALHTCRLAGLEHDAQCG
                     VLKRPLDPSRPQGTQIDLHVVVIPALARQKQPDPVFFFAGGPGQSAVGLAGTVQSLLQ
                     RLGQRRDLVLIDQRGTGHSAPLQCDIPPPDEALARALDRSREVAALQACRAKLQALPW
                     GDLRFYTTTIAMADADAVRVALGAEQVDLVGISYGTRAALEYLRVYPAHVRRAVIDGV
                     APPDMVLPASDDVDGNAALDKLFADCAADKACAQTHPALPQRWQALQASLPRAVDIHD
                     PVTGRPLHVTITTEAVRGAVRRPLYQPAQAAILPYAIEEAAAGRFDVLMAIASSTNDL
                     PTDLSEGQHFSVICSEDAPRLPAAGGPASDEGAQGLYRAVCAQWPRGE"
BASE COUNT          393 a         1024 c          999 g          385 t
ORIGIN      
        1 gcatccggcc gcgctgcagc gggcgcgcga gatcgcgaag aaggtgaccg acgacctcgg
       61 cggcctcggc ctcttcggcg tcgagctgtt cgtgaagggc gagcaggtct ggttcagcga
      121 agtctcgccg cgcccgcacg acaccggcat ggtgacgatg atcacgcagt ggcagagcga
      181 gttcgagctg cacgcccgcg cgatcctcgg cctgccggtg gacgtgtcgc tcaagagccc
      241 gggcgccagc gcggtgatct acggcggcgt cgacgcgaag gcggtgcgct tcgacggcgt
      301 cgccgacgcg atgcgcgtgc cgcagaccga gctgcgcctg ttcggcaagc ccgagagctt
      361 cgagcgccgc cgcatgggcg tggcgctcgc gcacgacacg gacgtcgagc gcgcgcgcgt
      421 caaggccaag gaggccgcgg ccaaggtcaa gcccagggcg gcctgacctc ggccctgcga
      481 atcacttgac caggccttcg gccttcatcg cgtcctgcac cgcgggacgc gccttcatgc
      541 gcgccatgaa cgcctcgagg ttcttcaggc ctgcgaggtc gacgccgacg cccgcggccc
      601 agttcgtcac cgtgaacagg taggcgtcgg cgacgctgaa ctgcttgccc atcaggtagt
      661 cgttgccctc caggcgcgcg tcgacgagcc ggtagcgctc ctgcagccgc gccgcgaaga
      721 cgtccttggc gtccttcgcc agcttcgggt tgaacatcgg cgagaagccc ttgtgcagct
      781 cgctggtgat gaagttgagc cactccatca cgcgatagcg ttccatcgtg ccggccgccg
      841 gcgcgaggcc gctggccggc gcgtggtcgg cgatgtactg caggatgacc gggccctcgc
      901 tcagccgcgc gccgtcgtcg agctcgagca gcggcacctg gcccttggcg gtgatctggt
      961 agtagtcgct gccgtcggcg agcgtgtgcg tctgcaaggc ggtcttgacg aggtcgaact
     1021 tcacgcccgc ctcgcgcagc gcgatgtggg gcgacagcga gcatgcgccg gggctgtaat
     1081 aaagctccat gacggtcttt cggtgaatgg ggaacgcgcc ggactgcccg gcgtgggcgg
     1141 gcgcccatga tgcccaaaac gccgccatgt tgcagggcag ggcattcgac gaaacgacgt
     1201 ggatcgccgc cgaacgggag cggggcgaca ggcgggtctg ctacgctgcg agccagctca
     1261 acgacaggct ccgcaccgtg acccgaccga tcctcgacgt ctcccgcatc caccccgcca
     1321 tccgcgcccg catcgccgac gaaggccgcg ccgtgatcgc cgaggtgcag gccgcgatcg
     1381 cggccaacaa ggtggtcgtc gtgggcatgg gctggaaccc gttcccgcgc aaggcgcgca
     1441 agtggctcga cgccgccggc gtcccgtacc agtacatcga gtacggcaac tacgcgagcc
     1501 aatggcgcaa gcgcctgccg ctcaagctgt ggacgggctg gacgacgttc ccgatggtgt
     1561 tcgtcgacgg ccagctcgtg ggcggcttcc gcgacctcgc caggctcgac ccggccacgt
     1621 tgaagaaggg ggcgggcgcc gcgtccgcgc atccgtgacc cggcgcttcg tccggcgcgc
     1681 ggcgctcgcc gcggcgctgg cgttcgccgg cgcaggcgcc tggggcgcca cgctggcgct
     1741 gcacacctgc cgcctcgccg gcctcgagca cgacgcgcag tgcggcgtgc tcaagcggcc
     1801 cctcgacccg agccggccgc agggcacgca gatcgacctg cacgtggtcg tcattccggc
     1861 gctcgcccgc cagaagcagc ccgacccggt cttcttcttc gccggcggcc ccggtcagag
     1921 cgccgtcggc ctcgccggca cggtgcagtc gctcctgcag cgcctcggcc agcggcgcga
     1981 cctggtgctg atcgaccagc gcggcaccgg ccacagcgcg ccgctgcagt gcgacatccc
     2041 gccgcccgac gaggcgcttg cccgtgcgct cgatcgctcg cgcgaggtgg cggcgctgca
     2101 ggcctgccgc gccaagctgc aggcgctgcc ctggggcgac ctgcgcttct acacgaccac
     2161 catcgcgatg gccgacgccg acgccgtgcg cgtcgcgctc ggcgcggagc aggtcgatct
     2221 cgtgggcatc agctacggca cgcgcgccgc gctcgaatac ctgagggtgt atccggcgca
     2281 cgtgcggcgc gcggtcatcg acggcgtggc gccgcccgac atggtgctgc ccgccagcga
     2341 cgacgtcgac ggcaatgcgg cgctcgacaa gctgttcgcc gactgcgccg ccgacaaggc
     2401 ctgcgcgcaa acgcatccgg cgctgccgca gcgctggcag gcgctgcagg cctcgctgcc
     2461 gcgtgccgtc gacatccacg acccggtcac cgggcgcccg ctgcacgtga cgatcacgac
     2521 cgaggccgtg cgcggcgccg tgcgccggcc gctgtaccag ccggcgcagg cggcgatcct
     2581 gccctacgcg atcgaggagg cggccgccgg ccgcttcgac gtgctgatgg cgatcgcatc
     2641 gtcgacgaac gacctgccga ccgacctgtc cgaaggccag cacttctccg tgatctgctc
     2701 ggaggacgcc ccgcgcctgc ccgcggccgg cggcccggcg agcgacgagg gcgcgcaggg
     2761 cctgtaccgc gccgtgtgcg cgcaatggcc gcgcggcgag g
//
LOCUS       DATCRO010000310         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_329721, whole genome shotgun sequence.
ACCESSION   DATCRO010000310 DATCRO010000000
VERSION     DATCRO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329721"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIN75_08420"
     CDS             complement(<1..227)
                     /locus_tag="VIN75_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974229.1"
                     /translation="MFSRTKICTALFAAMAANAWAQNAAPAANAPEATQRVEITGSAI
                     RRVETEGALPVTVLSRDEIDKSGAASITELI"
     gene            complement(458..943)
                     /locus_tag="VIN75_08425"
     CDS             complement(458..943)
                     /locus_tag="VIN75_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEY8974230.1"
                     /translation="MARKNHVSETPATQWLRAQGVAFTEHVYDYVEHGGTGESSAQLG
                     VPEHEVVKTLVMQDEAARPLVVLMHGDRTVSTKNLARAIGAKSVEPCKPEVAQRHSGY
                     QVGGTSPFGFRKEVPVYVQETILALPRILINGGRRGYLVGIPSAVLVDKLGAKPVQCA
                     N"
     gene            1134..1694
                     /locus_tag="VIN75_08430"
     CDS             1134..1694
                     /locus_tag="VIN75_08430"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEY8974231.1"
                     /translation="MAQPPRGGVPDELAQRLRRLALLVRVLIVVGALLTVGCAAWVWV
                     VPGHAHSQIKEAADVDIDQMALHTQVLGGLWTLLPVGIALLALQRLWRLFGEYARGRV
                     FSQRALLDLRGFARCVLTLAFVSPIYGALLSVIVTFDRQPGTRQLNLQFTSGDFAMLL
                     IGAVLLAIASVMAEAARVAEDNAGFV"
     gene            1696..1941
                     /locus_tag="VIN75_08435"
     CDS             1696..1941
                     /locus_tag="VIN75_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8974232.1"
                     /translation="MAIIVNLDVMLARRKMRSRELAERVGITEQNISLLKSGKVRGVR
                     FDTLAAICEVLQCQPGDLLEYRPGDAPAAADGEPGDD"
BASE COUNT          298 a          699 c          716 g          389 t
ORIGIN      
        1 tggatcagtt cggtgatcga agcggcgccg gacttgtcga tctcgtcgcg cgagaggacg
       61 gtgaccggca gggcgccttc ggtctcgacg cggcggatcg cggaaccggt gatctcgacg
      121 cgctgcgtgg cttcgggagc gtttgcggcg ggagcggcgt tctgggccca ggcgttcgcg
      181 gccatggcgg cgaacaaagc agtacagatc ttggtgcggc tgaacatgga gttccttcct
      241 cgtgggtggg gtcgttcttg tccgatcaac cgcgttgttc gtatcctttt gttaaggaaa
      301 cattacaacg ggtccctcag gtgcgcgatt gtgtgcattg ccacaatgtg aaccctccca
      361 gcacaaaccc tgctgtcagt ttttcgccgc gcaaattgac tagacaagcg gtccgtgcat
      421 cgaaaaaccg caccatgttc ggcgcttcag cgcaccttca gtttgcgcat tgcaccggct
      481 tggcgcccag cttgtcgacg agcaccgccg aggggatccc gaccaggtag ccgcgccgtc
      541 cgccgttgat gaggatgcgc ggcaacgcga ggatcgtctc ctgcacgtac accggcacct
      601 ccttgcggaa accgaacggc gatgtgccgc cgacctggta gccgctgtgg cgttgcgcca
      661 cctcgggctt gcacggctcg accgacttcg cgccgatcgc gcgcgccagg ttcttggtcg
      721 acaccgtgcg gtcgccgtgc atcagcacga cgagcggccg cgccgcctcg tcctgcatca
      781 ccagcgtctt gacgacctcg tgctcgggca cgcccagctg cgccgaggat tcgccggtgc
      841 cgccgtgctc gacgtagtcg tagacatgct cggtgaaggc gacgccctgc gcacgcagcc
      901 actgcgtggc gggggtttcg ctgacgtggt tcttgcgggc catgcgcgga ttgtcgcccg
      961 gccgcccggg ccggttcctg atggcctgtc aatgggattg cggtcgaggg ctcatcggag
     1021 taaagggccg tgcccatcgg ggtcttggtg catggctcgg agaccttatc attgcatgat
     1081 gatgttttat cgttttacaa taaattcttg tcgaactgcg ttcaacgtca cccatggccc
     1141 aacctccccg cggcggtgtc cccgacgagc ttgcccagcg cctgcggcgg ctcgcactgc
     1201 tggtgcgcgt cctgatcgtc gtcggcgcat tgctgacggt gggttgcgcc gcgtgggtgt
     1261 gggtcgtgcc cggccacgcg cactcgcaga tcaaggaagc cgccgacgtc gacatcgacc
     1321 agatggcgct gcacacgcaa gtgctgggcg ggctctggac gctgctgccg gtgggcatcg
     1381 cgctgctggc gctgcagcgc ctgtggcggc tgttcggcga gtacgcgcga ggccgcgtgt
     1441 tcagccagcg cgcgctgctg gacctgcgcg gcttcgcgcg ctgcgtcctg acgctggcgt
     1501 tcgtctcccc gatctacggc gcgctgctgt cggtgatcgt cacgttcgac cgccagcccg
     1561 gcacgcgcca gctcaacctg cagttcacgt cgggcgactt cgcgatgctg ctgatcggcg
     1621 ccgtgctgct ggccatcgcg agcgtgatgg ccgaggctgc ccgcgtggcc gaggacaacg
     1681 cgggattcgt ctgacatggc catcatcgtc aacctcgacg tgatgctcgc ccgacgcaag
     1741 atgcgttcgc gcgagctcgc cgaacgcgtg ggcatcaccg aacagaacat ctcgctgctc
     1801 aaatcgggca aggtgcgcgg cgtgcgcttc gacacgctgg ccgccatctg cgaggtgctg
     1861 cagtgccagc ccggcgacct cctcgaatac cggcccggcg acgccccggc cgcagccgac
     1921 ggcgagcccg gcgatgactg agcgcgcgcc cgcggccgcc gactgggcga gcctgcgacg
     1981 gcgccgctgg agcgtcgcgc ggctcgccgc cgcggcggcg ttgaacctgc tggtgctcgc
     2041 gtcgctggaa ctcgcggtcg accgcgtcga gctggcgccg cacggcccgg atcgcgtgac
     2101 gc
//
LOCUS       DATCRO010000311         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_37008, whole genome shotgun sequence.
ACCESSION   DATCRO010000311 DATCRO010000000
VERSION     DATCRO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37008"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="VIN75_08440"
     CDS             <1..2770
                     /locus_tag="VIN75_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8974233.1"
                     /translation="APEATQRVEITGSAIRRVETEGALPVTVLSRDEIDKSGAASITE
                     LIQKLPAMTGGNFQQSSSSVNGNGNGNTTAAIHGLDQKYTLVLLNGRRVAPFGGFGAS
                     GGDGSVNLSTLPLDAIERVEVLTDGASALYGSDAIAGVVNFITKKNTTDGLFSVTGSK
                     PHLAGGAKWSAGISKGFGDLDTTGNNLFLSYSHDVQKQLMASQRAVSRRGGLIPITID
                     GQPAILDQTSRNSLPGNVYLDSGLSFNAAFNQNGDCGDNPAVFVAGTKCRTNYAATVQ
                     DIPSTRRDSFYVSDHFKLNETNTLFAEYMWSKFDMTAAFAPPAQPMGLGSHDLPGRPL
                     DILWNKYVVPYENAHGDTSAGGTLYYRAFEAGDRTDQWETVANHYVLGAEGTAFNWDY
                     TASFTVSKNQDSDNLAGGYMDFNKFVNLIASGAYDPMVPKDGQTLAPALLSGTFLHTK
                     VAQDVLNLHASKDLFDLPGGRSSLALGFDYTRQSMEQSTSELAEFGNGTAAQAGKTDY
                     AVGGFYGYVPMEARRSNYGVFGELLMPIAKKTELTASVRFDRYDRVHNEKPFTQVPNN
                     LSPRPAEQEGNSFSHPTFKLSFRSQPAEMLLLRGSFGTGFKAPSINQIAAPLAFSTNT
                     SGSYECPFPTAPACVAHGPGADQWDLIALGNPASGAAGLKAETSKQWTLGGRLEPTKS
                     ISLGLDYWAVKLKNQILSGMPEGYAFANAAALQSLFVVPYTDPAGYQTVGLLQKPFNA
                     GSANYQGLDWDIALNSRTPFGKITADLGGTHMLKADYELPGGKQTDLGRFGGDNNVTF
                     RDIWRLALGLEYGDFTHTLIVNYKSGYADQAFTADSGNVSALLPDGSEGDGVAYNGHV
                     RSYKVVDWQTRWQASKNLRVTFGITNLFDEKPPLSLKIVGGNQVGYDGRYADPTGRAV
                     VVGANYKF"
     gene            complement(2923..>3929)
                     /locus_tag="VIN75_08445"
     CDS             complement(2923..>3929)
                     /locus_tag="VIN75_08445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY8974234.1"
                     /translation="DADGSGQCIGIIELGGGYRPADITAYFAALGIPTPKVKAVLVDH
                     AKNHPTNANSADGEVMLDIEVAAAIAPKATIAVYFAPNTTAGFLDAITKAVHDDVNKP
                     SVISISWGASESNWTAQAMTQYDQAFQAAAAMGVTICAASGDNGSSDGVPDGKVHVDF
                     PASSPNVLACGGTKLLASSATRISSEVVWNEGATTSATGGGVSGFFALPSYQGKAGVP
                     VAAGAGGKAGRGVPDVAGDADPSTGYEVRVDGENMVIGGTSAVAPLWAGLIALLNQKL
                     GHPVGLINPLLYGSLAGKGATHDITSGNNGSYSAKAGWDPCTGWGSPDGAKLLKALGT
                     "
BASE COUNT          642 a         1398 c         1302 g          587 t
ORIGIN      
        1 cgctcccgaa gccacgcagc gcgtcgagat caccggttcc gcgatccgcc gcgtcgagac
       61 cgaaggcgcc ctgccggtca ccgtcctctc gcgcgacgag atcgacaagt ccggcgccgc
      121 ttcgatcacc gaactgatcc agaagctgcc ggcgatgacc ggcggcaatt tccagcagtc
      181 ctcgagctcc gtcaacggca acggcaacgg caacaccacg gcggccatcc acggcctcga
      241 ccagaagtac acgctggtgc tgctcaatgg ccggcgcgtc gcgccgttcg gcggcttcgg
      301 tgcctcgggc ggcgacggtt ccgtgaacct gtcgacgctg ccgctcgacg ccatcgagcg
      361 ggtggaagtg ctgaccgacg gcgcgtcggc gctgtacggc tccgacgcca tcgccggcgt
      421 ggtgaacttc atcaccaaga agaacacgac ggacggcctg ttctccgtca ccggcagcaa
      481 gccgcacctg gccggcggtg ccaagtggag cgcgggcatc agcaagggct tcggcgacct
      541 cgacaccacg ggcaacaacc tgttcctgtc ctacagccac gacgtgcaga agcagctgat
      601 ggccagccag cgcgccgtct cccggcgcgg cggcctgatc ccgatcacga tcgacgggca
      661 gccggcgatc ctcgaccaga cctcccggaa ttcactgccg ggcaacgtgt acctggacag
      721 cggcctgtcg ttcaacgcgg ccttcaacca gaacggggac tgcggggaca acccggccgt
      781 gttcgtggcc ggcaccaagt gccgcaccaa ctacgcggcg acggtgcagg acatcccgag
      841 cacccgccgt gacagcttct acgtgagcga tcatttcaag ctcaacgaga ccaacaccct
      901 gttcgccgag tacatgtggt cgaagttcga catgaccgct gcgttcgcgc cgccggccca
      961 gccgatgggc ctgggatcgc acgacctgcc gggccggccg ctcgacatcc tctggaacaa
     1021 gtacgtcgtt ccctacgaga acgcccatgg cgacaccagc gccgggggta ccttgtacta
     1081 ccgcgccttc gaggccggcg accgcaccga ccagtgggag acggtggcca accactacgt
     1141 cctcggcgcc gaaggcaccg cgttcaactg ggactacacc gccagcttca ccgtgtccaa
     1201 gaaccaggat tcggacaacc tcgccggcgg ctacatggac ttcaacaagt tcgtgaacct
     1261 gatcgcgtcg ggcgcctacg accccatggt gcccaaggac ggccagacgc tggccccggc
     1321 cctgctgagc ggcacgttcc tgcacaccaa ggtcgcccag gacgtgctca acctgcacgc
     1381 gtccaaggac ctgttcgacc tgccgggtgg ccgctcttcg ctggcgctcg gcttcgacta
     1441 cacgcgccag agcatggagc agtcgaccag cgagctggcc gagttcggca acggcaccgc
     1501 ggcccaggcc ggcaagaccg actacgccgt cggcggtttc tacggctacg tgccgatgga
     1561 agcccgccgc agcaactacg gtgtgttcgg cgaactgctg atgcccatcg ccaagaagac
     1621 ggagctcacg gcctcggtgc gcttcgaccg ctatgaccgc gtccacaacg agaagccttt
     1681 cacgcaggtc cccaacaacc tgtcgccgcg gccggccgag caggaaggca acagcttcag
     1741 ccacccgacc ttcaagctga gcttccgctc gcagccggcc gagatgctgc tgttgcgcgg
     1801 ctccttcggc acgggcttca aggcgccctc gatcaaccag atcgccgcgc cgctggcctt
     1861 cagcaccaac acctcgggtt cctacgagtg tccgttcccg accgccccgg cctgcgtggc
     1921 gcacggtccg ggagccgacc agtgggacct gatcgccctg ggcaacccgg ccagcggcgc
     1981 cgcgggcctg aaggccgaga cgtccaagca gtggacgctc ggtggccgcc tggagccgac
     2041 gaagtcgatc tcgctgggcc tcgactactg ggccgtgaag ctgaagaacc agatcctgtc
     2101 gggcatgccc gaaggctacg cgttcgccaa cgcggcagcg ctgcagagcc tgttcgtggt
     2161 cccctacacg gatccggccg gctaccagac ggtgggcctg ctgcagaagc cgttcaacgc
     2221 cggcagcgcc aactaccagg gcctggactg ggacatcgcc ctgaacagcc ggacgccgtt
     2281 cggcaagatc acggcggacc tcggcggcac gcacatgctc aaggccgact acgagctgcc
     2341 cggcggcaag cagaccgacc tgggacgctt cggcggcgac aacaacgtca cgttccgcga
     2401 catctggcgc ctggcgctgg gcctggagta cggcgacttc acgcatacgc tcatcgtgaa
     2461 ctacaagtcg ggctatgccg accaggcgtt cacggccgac tcgggcaacg tctcggccct
     2521 gctgcctgat ggctcggaag gcgacggcgt ggcgtacaac ggccatgtcc ggtcgtacaa
     2581 ggtcgtcgac tggcagacgc gctggcaggc ctcgaagaac ctgcgcgtca ccttcggcat
     2641 caccaacctg ttcgacgaga agccgccgct gtcgctgaag atcgtgggcg gcaaccaggt
     2701 cggctacgac ggccgttacg ccgacccgac gggtcgcgcg gtcgtggtcg gcgccaacta
     2761 caagttctga ccccgcggca ggttgcggcc acgtgccgca acgacgacgc cccggcgcgc
     2821 gagcggccgg ggcgtctttc attcgacctc gcgcgtgcgc cagggcgcgt gaggcgcggg
     2881 caggcgcgcg cgcgtggccc gcgcgcggcg ccgcccggtt tttcaggttc ccagcgcctt
     2941 cagcagcttg gccccatcgg gcgagcccca gccggtgcac ggatcccagc cggccttggc
     3001 gctgtagctg ccgttgttgc ccgacgtgat gtcgtgcgtc gcgcccttgc cggcgagcga
     3061 gccgtacaac agcgggttga tcaggcccac cgggtggccc agcttctggt tcaggagcgc
     3121 gatcaggccg gcccacagcg gcgcgacggc gctggtgccg ccgatgacca tgttctcgcc
     3181 gtcgacacgc acctcgtagc cggtggacgg gtcggcgtcg ccggccacgt cgggcacgcc
     3241 gcgcccggcc ttgccgcccg cgccggcggc cacgggcacg ccggccttgc cctggtacga
     3301 cggcagcgcg aagaagccgc tgacaccgcc gccggtggcg ctggtggtgg cgccttcgtt
     3361 ccacacgacc tcggacgaga tcctggtggc gctggaggcc agcagcttgg tgccgccgca
     3421 cgcgagcacg ttcgggctgg acgcggggaa gtcgacgtgc accttgccgt cggggacgcc
     3481 gtccgacgag ccgttgtcgc cggaggccgc gcagatcgtc acgcccatcg cggccgcggc
     3541 ctggaacgcc tggtcgtact gcgtcatcgc ctgggccgtc cagttcgatt ccgacgcgcc
     3601 ccagctgatc gagatcaccg acggcttgtt gacgtcgtcg tgcacggcct tcgtgatggc
     3661 gtcgaggaag ccggccgtgg tgttgggcgc gaagtagacg gcgatggtcg ccttgggcgc
     3721 gatggcggcg gcgacctcga tgtccagcat cacctcgccg tccgcgctgt tggcgttggt
     3781 ggggtggttc ttggcgtggt cgacgagcac cgccttcacc ttgggcgtgg gaatgcccag
     3841 tgcggcgaag tacgcggtga tgtccgcggg ccggtagccg ccgcccagct cgatgatgcc
     3901 gatgcactgg cccgagccgt cggcgtcgg
//
LOCUS       DATCRO010000312         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1004721, whole genome shotgun sequence.
ACCESSION   DATCRO010000312 DATCRO010000000
VERSION     DATCRO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004721"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="VIN75_08450"
     CDS             <1..1993
                     /locus_tag="VIN75_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8974235.1"
                     /translation="FLVEARTLATFRHGNIVRVARFFEANRTAYMVMDYERGSPLLNW
                     WAEQFNAPPAAAPDSVPPSQRPAPGITEADLVMLLAPLLDGLALVHRTGVLHRDIKPG
                     NICVRESDGSFVLLDFGAARPAAGDLPDAPSMLTPGYAPIEQYLGDNQGPWTDLYAVG
                     ATLYWMVSGKRPRDAMDRKRTPQADISAEEAGAGRFGPDFLRAIDWALQVRAEDRPRD
                     VGSFRRALFAAHASSLGLQEALAGDADAPGAGRGLVARLLAHRALHPTSWPLVVKMVM
                     ALVLAAVLPMSITAWYNYTQNVDALANSERRNLERLALATSGRLAQLVEDSRRVTAFI
                     AAGPDVADLLAHPADPDKRALLLARLNSIVGTNADLHLLTLMDARGTALVSTAPEVVG
                     GNYAFREYYKVAMGGHSFITSVIVGSVAGRAGVYIAYPVMHEGQAIGVVTLRLRAEAI
                     DAIVDGVRNMNQEPFLVDGDGVLIHHRIERLRFHSLADLRPQAQERIAADQRFRLKHV
                     DSLGMPMLAGAMIGAKGEGTIDYESSISRTREVAGFAPVAGVDWVVGVTESRDSFESP
                     LRLIFVQLLVSVAAVGTFFVLLAIVFARSISRPVQALTHAADALKRGDYDAARVRVAS
                     GDEIGALGRTFNVMIDVLRQREREHGLREGRGDKGRPRR"
     gene            2074..2442
                     /locus_tag="VIN75_08455"
     CDS             2074..2442
                     /locus_tag="VIN75_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY8974236.1"
                     /translation="MSSAMQTVTSSNPVITSDRVAGTDVFNPAGDSLGSIDYLVIDKH
                     SGRIRYAVMEFGGILGMGADRYPLPWSMLRYDVDKEGYVVPLEKEQLGDAPRHPIDAR
                     PSYDESYSRAVHEHYGAPWA"
     gene            2800..3051
                     /locus_tag="VIN75_08460"
     CDS             2800..3051
                     /locus_tag="VIN75_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974237.1"
                     /translation="MPDRILRTVHGLRWDIKILWRLDNGAWSMFGDAPDEVVSRWAAT
                     DPNTVHRHQLHNAILAARDELALRERQVPVEFGSAMPVV"
     gene            complement(3221..3311)
                     /locus_tag="VIN75_08465"
     tRNA            complement(3221..3311)
                     /locus_tag="VIN75_08465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3275..3277),aa:Ser,seq:gga)
     gene            3543..>3973
                     /locus_tag="VIN75_08470"
     CDS             3543..>3973
                     /locus_tag="VIN75_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8974238.1"
                     /translation="MLRFRFPIVIIDEDFRSENTSGLGIRALAAAIEKEGIEVLGVTS
                     YGDLSQFAQQQSRASAFILSIDDEEFTPGPELDPAVLSLRKFIVEIRSRNPEIPIYLY
                     GETRTSQHIPNDILRELHGFIHMFEDTPEFVARHIIREATS"
BASE COUNT          586 a         1446 c         1364 g          577 t
ORIGIN      
        1 gttcctcgtc gaggcgcgca ccctggccac gttccgccac ggcaacatcg tgcgcgtggc
       61 gcgcttcttc gaggccaatc gcaccgccta catggtgatg gactacgagc gcggcagccc
      121 gctgctgaac tggtgggccg agcagttcaa tgccccgccg gcggccgccc ccgacagcgt
      181 gccgccgtcg cagcggccgg cgccgggcat caccgaggcc gacctcgtga tgctgctggc
      241 cccgctgctg gacggcctcg cgctcgtgca tcgcaccggc gtgctgcacc gggacatcaa
      301 gcccggcaac atctgcgtgc gcgagtccga cggcagcttc gtgctgctcg acttcggcgc
      361 cgcgcggccc gccgccggcg acctgcccga cgcgcccagc atgctcacgc cgggctacgc
      421 gcccatcgag cagtacctgg gcgacaacca ggggccgtgg accgacctct acgccgtggg
      481 cgcgacgctg tactggatgg tcagcggcaa gcgcccgcgc gacgccatgg atcgcaagcg
      541 cacgccgcag gccgacatca gcgcggagga ggccggggcc ggccgcttcg gcccggactt
      601 cctgcgtgcc atcgactggg cgctgcaggt gcgggcggaa gaccggccgc gcgacgtcgg
      661 cagcttccgc cgcgcgctgt tcgccgccca cgcgtccagc ctgggcctgc aggaggcgct
      721 ggccggcgac gccgacgcgc cgggcgcagg ccgcggcttg gtcgcccggt tgctggccca
      781 tcgcgcgctg catccgacgt cctggccgct ggtggtcaag atggtgatgg cgctggtgct
      841 cgccgccgtg ctgcccatga gcatcaccgc ctggtacaac tacacgcaga acgtggacgc
      901 gctcgccaac tccgagcggc gcaacctgga gcggctggcg ctggccacgt cggggcggct
      961 cgcgcagctc gtcgaggata gccggcgggt gaccgccttc atcgccgcgg gccccgacgt
     1021 cgccgacctg ctcgcgcatc cggccgaccc cgacaagcgc gcgctgctgc tggcgcgcct
     1081 gaacagcatc gtcggcacca acgccgacct gcacctgctc acgctgatgg acgcgcgcgg
     1141 cacggcgctg gtctcgaccg cgcccgaggt cgtcggcggc aactacgcct tccgcgagta
     1201 ctacaaggtg gcgatgggcg gccactcgtt catcaccagc gtcatcgtcg gctcggtcgc
     1261 ggggcgcgcg ggcgtctaca tcgcctaccc ggtgatgcac gagggccagg ccatcggcgt
     1321 cgtcacgctg cggctgcgcg ccgaggcgat cgacgccatc gtcgacggcg tgcgcaacat
     1381 gaaccaggag ccgttcctcg tcgacggcga cggcgtgctg atccaccacc gcatcgagcg
     1441 cctgcgcttc cacagcctgg ccgacctgcg gccacaggcg caggagcgca tcgccgccga
     1501 ccagcgcttc cgcctcaagc atgtcgacag cctgggcatg ccgatgctcg ccggcgcgat
     1561 gatcggagcc aagggcgagg gcacgatcga ctacgagtcg tcgatctcca ggacgcgcga
     1621 ggtcgcgggc ttcgcgccgg tcgcgggtgt cgactgggtg gtgggcgtga cggaatcgcg
     1681 cgacagcttc gagtcgccgc tgcgcctgat cttcgtgcag ctgctggtca gcgtggccgc
     1741 cgtcggcacg ttcttcgtgc tgctggcgat cgtgttcgcg cgcagcatca gccggcccgt
     1801 ccaggcgctg acgcacgcgg ccgacgcgct caagcgcggc gactacgacg cggcgcgcgt
     1861 gcgcgtggcc agcggcgacg agatcggcgc gctgggccgc acgttcaacg tcatgatcga
     1921 cgtgctgcgc cagcgcgaac gcgagcacgg gctgcgcgaa gggcggggcg acaaggggcg
     1981 gccgcgacgc tgaaggcgcg gcgcgcggtc gctcgggtac gcggcgtgcc atcgacgggc
     2041 caggtcgcgg ccaccgcccg cgacgttcct cccatgagca gcgccatgca gaccgtcacc
     2101 tcgtccaacc ccgtcatcac gtccgaccgc gtcgccggca cggacgtctt caaccccgcc
     2161 ggcgactcgc tcggctccat cgactacctc gtcatcgaca agcattccgg ccggatccgc
     2221 tacgcggtga tggagttcgg cggcatcctc ggcatgggcg ccgaccgcta cccgctgccg
     2281 tggagcatgc tgcgctacga cgtcgacaag gaaggctacg tcgtgccgct cgagaaggaa
     2341 cagctcggcg acgcgccgcg ccacccgatc gacgcgcgtc cgagctacga cgagtcgtac
     2401 agccgggccg tgcacgagca ctacggggcg ccctgggcct gaggccacgc cggcgtcgac
     2461 cggccggtcc gccggacccg cagcggcccc gggggtcgcg ccgcacgcct gcgcgacggg
     2521 tggcgaaatt ccctcttcct gccgggccgt tgcggcagaa accgccgctt gcacggcgaa
     2581 tccatccagc aggtcgatcg atcggtagcg gctactcgtg cattgcgaca cgcccgggag
     2641 gccgttcgcc ccgattcgag cgcgccgcat gcgggatcgc ccgcgttggc tcgaagattg
     2701 ctctgcacgt gcgccattgg ctcgcggccc catccgccgc gacgtcgagg cgcgcgcgac
     2761 ccgtcgccgc cgttgaatca acagaaagag ggtagcaacg tgccagaccg cattctccgc
     2821 accgtccacg gcctgcgctg ggacatcaag atcctctggc gcctggacaa cggcgcctgg
     2881 tccatgttcg gcgacgcccc cgacgaggtc gtcagccgct gggccgcgac cgacccgaac
     2941 acggtgcacc ggcaccagct ccacaacgcc atcctcgccg cccgcgacga gctggctctg
     3001 cgcgagcgcc aggtgccggt cgagttcggg agcgcgatgc cggtggtgtg agcgtggggt
     3061 gcgcggcgca ccgcttcgtg cgcttcgagg tcgagacggg cccgggactc gagccggcgg
     3121 ccagttcgtt ggaacgccgc gggatgcggc acgcggcgcg gtaatggcgc gcccggaaag
     3181 caaaaaaccc caagcaagtc gattgctcgg ggctcctgtc tggcggaagg ggcgggattc
     3241 gaacccgcgg taggctatta acctacacat gctttccagg catgcgactt aaaccgctca
     3301 tccacccttc cggggtagcc ggctattcta gctgatgaac gccggggtct gtccagtgcc
     3361 gtgacgctgt cgagacaccg gccgcggtcg ggcagggcgt ttgttgcgga atcgggtcgc
     3421 cggaccgggg gtgtgcccgg agatggcccg attgacattg cgcggaaagc gttcggccct
     3481 acacttcgcg cttcgcccgt tttccacccc gccacgcccg ccctccgagc gggcctcgtg
     3541 ccatgcttcg cttccgcttc ccgatcgtca tcatcgacga ggacttccgt tccgagaaca
     3601 cctcgggcct gggcatccgc gcgctcgccg cggcgatcga gaaggagggc atcgaggtgc
     3661 tgggcgtcac cagctacggc gacctgtcgc agttcgcgca gcagcagagc cgcgcgtcgg
     3721 cgttcatcct gtcgatcgac gacgaggagt tcacgcccgg tcccgagctc gatccggccg
     3781 tgctgagcct gcgcaagttc atcgtcgaga tccgctccag gaatccggag atcccgatct
     3841 acctgtacgg cgagacgcgc acgtcgcagc acatccccaa cgacatcctg cgcgagctgc
     3901 acggcttcat ccacatgttc gaggacacgc cggagttcgt ggcgcgccac atcatccgcg
     3961 aggccacgag cta
//
LOCUS       DATCRO010000313         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_824916, whole genome shotgun sequence.
ACCESSION   DATCRO010000313 DATCRO010000000
VERSION     DATCRO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824916"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1810)
                     /locus_tag="VIN75_08475"
     CDS             complement(104..1810)
                     /locus_tag="VIN75_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8974239.1"
                     /translation="MRTADNHASLHAGFAWSVPEHFNIAQVCLHRWARDTPAAVAIVQ
                     DAGDEPAVAFTYAALQRDARRLSNALGSLGVTRGDRVAIVMPQRPQTAVALMAVFQMG
                     AIALPLSTLFGAEVLQARLNDSGARLAIVDEASAGTIAALRGSCPALLRVMGAGAADG
                     QGDVSWDASLAAARDVFEPTDTRADDPALLIYTSGTTGPAKGAVIPHRALIGNLSGFV
                     ASQDWFEGDGPFWSPADWAWTGGLWDALLPTLYFGRTIVASRARFDADLALKLMERHR
                     VTHTFLFPTALKAMMRVAPEPRKSHPRLALKSIMSAGEAVGEGVFAYCRDKLGVVVNE
                     MFGQTEINYVVGNCGRYRAVDGTLAPVWPPRPGSMGRPYPGHRVAVIDDDGNECPRGT
                     PGDIAVHARDVHGARDPIFFLGYWNNERATRAKFTGKPFESWCRTGDRAVMDEDGYLW
                     YQGRSDDQFKSSGYRIGPTEIEDCLARHPAVAQVAVVPKPDPERGAVVKAFIMCTAGV
                     RPDAALVEALQQLVRSRLAPYETPKDIEFVDALPMTATGKLQRNVLRKLEEDRAAGRV
                     GA"
     gene            complement(1820..2323)
                     /locus_tag="VIN75_08480"
     CDS             complement(1820..2323)
                     /locus_tag="VIN75_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974240.1"
                     /translation="MIPATAPRATVRFATRADLPVIVSLIRELAVYEKLEHLATATPE
                     RLEPELFGDGSVIECLVGEQDGEPVGFALFFHNFSTFLCRKGLYLEDLFVRPSARGTG
                     MGKLLLQRLAQIAVERGCGRFEWSVLDWNVDAQAFYAKMGATMLPDWRICRVTGDALQ
                     ALGKPAA"
     gene            2382..3308
                     /locus_tag="VIN75_08485"
     CDS             2382..3308
                     /locus_tag="VIN75_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8974241.1"
                     /translation="MTSSNAYVERRPGRKRLIPIRNLHYQLREWGDPSLATPERPPLV
                     FTHGWMDVGASFQFIVDELERIEGGTRHVIAIDWRGFGGSTSGGADAYWFHDYLGDLD
                     AVLDALSPDAPVDLAGHSMGGNVVMNYAGARPARVRRLVNLEGFGLPDMKPEHAPERL
                     AKWLDELKAPPSLRSYDTVDDVAARLRANNPRLAADKAAWLAPHWSEQRADGRWHIRA
                     DPAHKLTNPVPYRAAEIVAGWRRIAAPVLWVEGADTDVTKYWGNRYPRAEFEERFAQV
                     PRVERLLLDDCGHMLHHDQPAAVAARLAAFLA"
     gene            3384..3920
                     /locus_tag="VIN75_08490"
     CDS             3384..3920
                     /locus_tag="VIN75_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974242.1"
                     /translation="MTKIVPLDPVPAPPAVADTGANRDPLSGEVGSHPIGVGLGAAAA
                     GMAFGAAVASVAGPIGTAVGAAVGAIAGGLAGKGVAEAIEPTAEDAYWRANYASRAYA
                     TPRDADDEWGPAYAYGVAHYNRNVVAHPEATFEELEPEMERGWAEARGRSSMEWTRAR
                     LAAYDAWQNARAADDRFA"
BASE COUNT          571 a         1470 c         1435 g          530 t
ORIGIN      
        1 gctcagcgcg ccctttctgc tcgccgccga ggcgctcggc aaatgcgcgc acaagctctg
       61 atcgtctgat cgcgccgcca tcgcggcggc ggaagttcga cgctcaggcg ccaacgcgac
      121 ccgcggcgcg atcttcctcg agcttgcgca gcacgttgcg ctgcagcttg ccggtggcgg
      181 tcatcggcag ggcgtcgacg aactcgatgt ccttgggcgt ctcgtagggc gcgaggcggc
      241 tgcgcaccag ctgctgcagc gcctccacca gcgcggcgtc gggccgcacg cccgccgtgc
      301 acatgatgaa ggccttgacg acggcgccgc gctcggggtc gggcttgggc acgacggcca
      361 cctgcgcgac cgccgggtgg cgcgcgaggc agtcctcgat ctcggtcggg ccgatgcggt
      421 agcccgagga cttgaactgg tcgtcgctgc ggccctggta ccagaggtag ccgtcctcgt
      481 ccatcaccgc gcggtcgccg gtgcggcacc acgactcgaa cggcttgccg gtgaacttgg
      541 cgcgggtggc gcgctcgttg ttccagtagc cgaggaagaa gatcgggtcg cgcgcgccgt
      601 gcacgtcgcg cgcgtgcacc gcgatgtcgc ccggcgtgcc gcgcggacac tcgttgccgt
      661 cgtcgtcgat caccgcgacg cgatggccgg ggtacggtcg ccccatgctg cccgggcgcg
      721 gcggccagac gggcgccagc gtgccgtcga ccgcgcggta gcggccgcag ttgccgacga
      781 cgtagttgat ctcggtctgg ccgaacatct cgttgacgac cacgccgagc ttgtcgcggc
      841 agtacgcgaa cacgccctcg cccaccgcct cgccggcgct catgatggac ttcagcgcga
      901 gccgcgggtg cgacttccgc ggctcgggcg cgacgcgcat catcgccttc agcgcggtcg
      961 ggaacaggaa ggtgtgcgtg acgcgatgcc gctccatcag cttgagcgcg aggtcggcgt
     1021 cgaagcgggc gcgcgacgcg acgatggtgc ggccgaagta cagcgtgggc agcagcgcgt
     1081 cccacaggcc gcccgtccac gcccagtcgg cgggactcca gaacggaccg tcgccctcga
     1141 accagtcctg gctcgcgacg aagccgctca ggttgccgat gagcgcgcga tgcgggatca
     1201 cggcgccctt ggccgggccc gtcgtgccgc tcgtgtagat cagcagcgca gggtcgtcgg
     1261 cgcgcgtgtc ggtgggttcg aagacatcgc gcgccgcggc cagcgaggcg tcccacgaca
     1321 cgtcgccctg gccgtcggcc gcgcccgcgc ccatcaccct cagcagcgcc gggcatgatc
     1381 cacgcagcgc ggcgatcgtg ccggccgagg cctcgtcgac gatcgcgagc cgcgctccgc
     1441 tgtcgttgag ccgcgcctgc agcacctccg cgccgaacag ggtcgacaac ggcagcgcga
     1501 tggcgcccat ctggaacacg gccatcaacg cgacggctgt ctgcgggcgc tggggcatga
     1561 cgatggcgac gcggtcgccg cgcgtcacgc ccagcgagcc gagcgcgttc gacagccgcc
     1621 gcgcgtcgcg ctgcaacgcg gcgtaggtga aggccacggc cggctcgtcg cccgcgtcct
     1681 gcacgatggc caccgcggcc ggcgtgtcgc gcgcccagcg atgcaggcac acctgcgcga
     1741 tgttgaagtg ctcggggacg ctccaggcga aacccgcgtg gagcgaggcg tggttgtcgg
     1801 cggttctcat ccgggcagct tacgccgccg gcttgcccag tgcttgcagt gcgtctccgg
     1861 tgacgcggca gatgcgccag tccggcagca tcgtcgcgcc catcttcgcg tagaacgcct
     1921 gcgcgtcgac gttccagtcg agcaccgacc actcgaagcg accgcagccg cgctccaccg
     1981 cgatctgcgc caggcgctgc agcagcagct tgcccatgcc ggtgccgcgc gccgacgggc
     2041 gcacgaacag gtcctccagg tacaggccct tcctgcacag gaacgtcgag aagttgtgga
     2101 agaacagcgc gaagccgacg ggctcgccgt cctgctcgcc gacgaggcac tcgatgaccg
     2161 agccgtcgcc gaacagctcc ggctcgaggc gctcgggcgt ggcggtggcc aggtgctcga
     2221 gcttctcgta gaccgcgagc tcgcggatca gcgacacgat gacgggcagg tccgcgcgcg
     2281 tggcgaagcg gacggtggcg cggggggcgg tggcaggaat catgtcggca ttgtcgcccg
     2341 caggtcagcg cgcgaacgcg aagtcctcta cagtgcaacg gatgacctcg tccaatgcct
     2401 acgtcgagcg gcggcccggc cgcaagcggc tgatcccgat ccgcaacctg cactaccagc
     2461 tgcgcgaatg gggcgacccg tcgctcgcca cgcccgagcg gccgccgctg gtcttcacgc
     2521 acggatggat ggacgtgggc gcgtcgttcc agttcatcgt cgacgagctc gaacgcatcg
     2581 agggcggcac gcgccacgtg atcgcgatcg actggcgcgg cttcggcggc agcaccagcg
     2641 gcggcgccga cgcgtactgg ttccacgact acctcggcga cctggacgcc gtgctggacg
     2701 ccctgtcgcc cgacgcgccc gtcgacctgg ccggccacag catgggcggc aacgtggtga
     2761 tgaactacgc cggcgcgcgg ccggcccggg tgcggcgcct ggtcaacctc gagggcttcg
     2821 gcctgcccga catgaagccc gagcacgcgc ccgagcgcct cgccaagtgg ctcgacgaac
     2881 tgaaggcgcc gccctcgctg cgctcctacg acacggtgga cgacgtcgcc gcgcggctgc
     2941 gcgccaacaa cccgcgcctg gccgccgaca aggccgcctg gctcgcgccg cactggtcgg
     3001 aacagcgtgc cgacggccgc tggcacatcc gcgccgatcc cgcgcacaag ctgaccaacc
     3061 cggtgccgta ccgcgcggcc gagatcgtcg ccggctggcg ccgcatcgcc gcgccggtgc
     3121 tgtgggtcga aggcgccgac accgacgtca cgaagtattg gggcaaccgc tatccgcgcg
     3181 ccgagttcga ggaacgcttc gcgcaggtgc cgcgcgtcga acgactgctg ctggacgact
     3241 gcggccacat gctgcaccac gaccagccgg ccgccgtcgc cgcgcggctg gcagcgttcc
     3301 tggcctgaac gtcccgccgc agcaggaacg gtggttgccc cgaccggccg atgcggcgcc
     3361 aacccgccgc gcaggagttc gccatgacca agatcgttcc gctggatccc gtccccgccc
     3421 cgcccgccgt cgccgacacg ggcgccaacc gcgacccgct gtcgggcgag gtcggctcgc
     3481 atccgatcgg cgtgggcctg ggtgccgccg ccgccggcat ggccttcggc gcggcggtcg
     3541 cgtcggtcgc cgggcccatc ggcaccgcag tgggcgctgc cgtcggcgcg atcgcgggcg
     3601 gcctggccgg caagggcgtg gccgaggcca tcgaaccgac cgccgaggac gcctactggc
     3661 gcgccaacta tgcctcgcgc gcctacgcca cgccgcgcga cgccgacgac gaatggggcc
     3721 cggcctacgc gtacggcgtc gcccactaca accgcaacgt cgtcgcccat cccgaagcca
     3781 ccttcgagga actcgagccc gagatggagc gcggctgggc cgaggcacgc ggccggtcca
     3841 gcatggaatg gacccgtgcg cggctggccg cctacgatgc gtggcagaac gcgcgggcgg
     3901 cggacgaccg gttcgcctga gcgggcgctg ggctcgcagg aaccgatggc gcaggccggc
     3961 gtgcgagcgc cggtcggtcg agggatccgc ccggagccat cgtgcc
//
LOCUS       DATCRO010000314         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_419982, whole genome shotgun sequence.
ACCESSION   DATCRO010000314 DATCRO010000000
VERSION     DATCRO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419982"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VIN75_08495"
     CDS             <1..426
                     /locus_tag="VIN75_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY8974243.1"
                     /translation="VFTNGVFDLLHRGHIAWLAEARQLGDRLVVGISGDTAARLADTP
                     HDGPLNPEGDRAFAVAALESVDAVVIFHEPTPIALLTRLRPSVYVKGGDEDMSKLAAS
                     ALVRGWGGHAVALPFVDVFSTASLMDRLRASSRLAPTES"
     gene            464..1051
                     /locus_tag="VIN75_08500"
     CDS             464..1051
                     /locus_tag="VIN75_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8974244.1"
                     /translation="MIPTVRRAVFLGRDGVLNRDHGHVSRPEEFEWLPGVMHALRALQ
                     RAGWGLVVVTNQGGIARGLYGPAEYANLTAWMHSELAQHGIALDEVYHCPHLADAPLA
                     AWRRQCDCRKPAPGLLRRAARDLSLDLASCVIVGGKPCDIQAGRAAGVAACIRITDPI
                     DPAAIATASPAADYTCTNLAEAARWLLEHEQTRSA"
     gene            1217..1292
                     /locus_tag="VIN75_08505"
     tRNA            1217..1292
                     /locus_tag="VIN75_08505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Thr,seq:cgt)
     gene            1903..2217
                     /locus_tag="VIN75_08510"
     CDS             1903..2217
                     /locus_tag="VIN75_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974245.1"
                     /translation="MPIATPRFSKWQAAEQAAQAVEGEIRKRLLEAPADAPRPDLDAE
                     LALAGELRRIAHALFEDAMIELDIVASALEQSALHAYSAYSAYSAYSADMRRSIPPSR
                     RN"
     gene            complement(2321..>2604)
                     /locus_tag="VIN75_08515"
     CDS             complement(2321..>2604)
                     /locus_tag="VIN75_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8974246.1"
                     /translation="AEHAALAGVVYYAVVPTVCGYLLWYAGAARVSAGVASLYTAVLP
                     VSALVLAAIVLREPVGAGQLIGTGCVLAAIAVGRPRGGGSQRGTDRHAG"
BASE COUNT          381 a          961 c          888 g          374 t
ORIGIN      
        1 gtgttcacca atggcgtctt cgacctgctg catcgcgggc acatcgcctg gctggccgag
       61 gcgcggcaac tcggcgatcg gctggtcgtc ggaatcagcg gcgacaccgc cgcccgcctt
      121 gccgacacgc cgcacgacgg cccgctgaac cccgaaggcg accgcgcctt cgccgtcgcc
      181 gcgctcgaga gcgtcgacgc cgtcgtgatc ttccacgagc ccacgccgat cgcgctgctg
      241 acccggctgc gtccgtcggt ctacgtcaag ggcggcgacg aggacatgag caagctggcg
      301 gcgagcgcgc tggtgcgcgg ctggggcgga cacgcggtgg cactgccctt cgtcgacgtc
      361 ttctccacgg cctcgctgat ggaccggctg cgtgcgtcgt cgcgcctggc gccgacggaa
      421 tcatgagcgg ctcgacatca tcggcgcgcg gcggggaggc ctcgtgatcc ccaccgtgcg
      481 tcgtgccgtg ttcctgggtc gcgacggcgt cctcaatcgc gatcacggcc atgtgagccg
      541 tcccgaggag ttcgagtggc tgcccggcgt gatgcatgcg ctgcgggcgc tgcaacgggc
      601 gggctgggga ctggtggtcg tcaccaacca gggcggcatc gcgcgcggac tctacgggcc
      661 ggccgagtac gccaacctga cggcctggat gcacagcgaa ctcgcgcagc acggcatcgc
      721 actcgacgag gtctaccact gcccgcacct ggccgacgcc ccgctggccg cctggcgccg
      781 ccagtgcgac tgccgcaagc ccgccccggg cctcctgcgt cgcgcggcgc gcgacctgtc
      841 gctcgacctc gcgtcctgcg tgatcgtggg cggcaagccc tgcgacatcc aggccgggcg
      901 cgccgcgggc gtggcggcct gcatccgcat caccgatccg atcgacccgg ccgccatcgc
      961 gaccgccagc ccggccgccg actacacctg cacgaacctt gccgaggcgg cgcgctggct
     1021 cctggaacat gagcagacgc gttcggcatg agcgggaggt ggcgcgtcca cggccgttgc
     1081 cgtccacggt cgagcggcac gacgcgcttg tgcccgggac gccacggctt gattcgatct
     1141 cttcttgcca ctgcgaaaat ctttgcgcta gaatggcagg cttcgcggta tcccacttgt
     1201 ggggcattgc tgaaacgccg gtgtagctca gttggtagag cagctcattc gtaatgagaa
     1261 ggtcgagggt tcgactcctt tctccggcac cagaatcatc gttgcagatc aacgacttgc
     1321 agcgatcgaa agccgacccc cgggtcggct ttttcgttcc tggctcgctt tgacgcccga
     1381 ccgcggccat cgggccatcg cgtcgagaga agctcgctcc aacgaggcgc cgggccggcc
     1441 gggtcgacgg gcggccgttg cacacccgat gccgtctgcg cgagaactcc tgcatcgttt
     1501 tcaaacctcg ccgtggctgc gacgcctccc ccctcggcgt gcgcggccac tcgacccacg
     1561 cggcgtctcg gcagttcgtc gcggggcctt caaggacgag gacggccggg tcgggacgcc
     1621 gttcctgcaa catcgagacg cgatgatggc gggcaagagg caccagccgg ccatgccggt
     1681 cgtcgatctg cgtcgtgcgc gtgggaggcg aaggcgggtc gcgcccaggg ctgacctgcc
     1741 ctgcgaaggc gcgcggcgtg tgcggtgacg cccccgcctg gccggccgga ttgcggggca
     1801 gcggactggc ggcgtaaagt cgagcctcgg acggcttgcc gcgcgacacc gccggggcga
     1861 ccgccccccc ctctcgcagc tcagcgttcg aggattcgat cgatgccgat cgcgacaccc
     1921 aggttcagca agtggcaggc ggccgaacag gccgcgcagg cggtcgaggg cgagatccgc
     1981 aagcgcctgc tcgaggcgcc agcggacgcg ccgcggcctg acctggatgc ggaactggcg
     2041 ctggccggcg agctcaggcg catcgcccat gcgttgttcg aggacgcgat gatcgagctg
     2101 gacatcgtcg catcggccct ggaacagagc gcgctgcacg cctactccgc ctactccgcc
     2161 tactccgctt actctgccga catgcgccgg agcatcccgc caagccggcg caactgaggt
     2221 gcccgcgcgc cttcgcccgc gctccggcgc gacgacaccc gatcaatcgc gggcccgggg
     2281 cacgctgacc gtgacccagc tcccatgacg ccgggccgcg ctacccggca tggcgatccg
     2341 tgccacgttg cgacccgccc ccacgaggcc gcccgaccgc gatcgcggcc agcacgcacc
     2401 ccgtgccgat caactgtccg gcgccgacgg gttcgcgcag cacgatcgcc gcgagcacca
     2461 acgccgacac cggcagcacc gcggtgtaga gcgacgccac gcccgcgctg acccgggctg
     2521 cgcccgcata ccacagcagg tagccgcaga ccgtcggcac gaccgcgtaa tacaccacgc
     2581 ccgccagcgc ggcgtgctcg gcgg
//
LOCUS       DATCRO010000315        10744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_487157, whole genome shotgun sequence.
ACCESSION   DATCRO010000315 DATCRO010000000
VERSION     DATCRO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487157"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VIN75_08520"
     CDS             <1..1207
                     /locus_tag="VIN75_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8974247.1"
                     /translation="GNMERVLHLMADKGASDVYLTANSPIQLKINGQLVPVSDQILTP
                     GQPRALLAELLTPVQLEELDQTGELNLGIGIRAVGSFRLSAFKQRASIAAVIRCIPHK
                     IPSMDTLNLPPVLASLALERRGLVLVVGATGNGKSTSLASMIEWRNTQLSGHILTIEE
                     PIEFLFSNKRSIINQREVGRDTASLHIALKNALRQAPDCILIGEIRDRETMSMAISYA
                     LSGHLVLATLHANNSSHTLGRILSFYTPEARPALLGDLAAALKAIVSQRLLRSTAGGR
                     VPAVEVLLNSRFIAELIENGDIQGVREAMDKSLAEGSQMFEADIARLIGQGLVTQEEG
                     LLGADSPTNLLWRLQNEMTPVSRVQPAKVESDAPSFTEITLDVIPEEPAPRQRLGGVA
                     VEMHRSKHG"
     gene            1231..2391
                     /gene="dapE"
                     /locus_tag="VIN75_08525"
     CDS             1231..2391
                     /gene="dapE"
                     /locus_tag="VIN75_08525"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY8974248.1"
                     /translation="MDATLPLLEQLIARPSVTPDDAGCQALIAQRLQAIGFACEHLPF
                     GPADGIVSNLWAVRAGARPGPTLVFAGHTDVVPTGPHDQWSSAPFVPSHREGKLYGRG
                     TADMKTSLAAMVVAAEEFVAAHPDHAGRVAFLLTSDEEGPSVDGTVRVVDTLQARGEK
                     LDCCIVGEPTCVERLGDMIKNGRRGSLSARLTVVGVQGHVAYPHLARNPIHVAAPAIA
                     ELAATRWDDGNDHFPPTSFQISNIHAGTGATNVIPGEAVIDLNFRFSTESTPESLKAR
                     VVEVLQRHGVEHRVAWTLGGSPFLTRPGTLSEALVQAIVDETGGVRAELSTTGGTSDG
                     RFISRICPQVVEFGPINATIHKIDEHVDVADVPRLKNIYRRAMENLLVPNAT"
     gene            2388..3287
                     /gene="prmB"
                     /locus_tag="VIN75_08530"
     CDS             2388..3287
                     /gene="prmB"
                     /locus_tag="VIN75_08530"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8974249.1"
                     /translation="MTLIDYLHRAGARLDEAGVSFGHGTTNAFDEAAWLVLWKLGLPL
                     DDLDGVARRELTDVEIATLDAFVGERIATRRPAAYLTGEAWLQGVPFTVDERVIVPRS
                     LIAEVLAEGVIDAWMPPDTARVLDLCTGNGSLAVLAAMAWPDVAVDAADLSVDALAVA
                     ALNVKRHDLAGRIALHRGDGLAALPAGRRYDLILCNPPYVNAQSMSELPAEYKAEPDM
                     ALAGGDDGMDFIRAMFAAAPDRMTDDAVLVLEIGNERDFFEAAFPGLEVVWLPTQLHD
                     DAVLLVTRAMLEDGLAPLPKHGS"
     gene            3290..5347
                     /locus_tag="VIN75_08535"
     CDS             3290..5347
                     /locus_tag="VIN75_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974250.1"
                     /translation="MITLRNVQLRRGTKIVLDGVNVNVNPGEKVGLVGRNGAGKSSLF
                     ALLAGRLQADQGDFEIPARWRLGEVAQHMPETEDSATDFVLEGDIPLMEAQRELAAAE
                     AAEDGEAMAHAHMKIGEAGGFDARARAQALLMGLGFKNEQVDAPVNSFSGGWRMRLQL
                     ARALMCPADLLLLDEPTNHLDLDALVWLESWLQRFDGTIVMISHDREFLDAITGVTVH
                     LDEGTLTRYGGNYSTFEEMRAERMSQQAAAHSRQQEKVAQLQRFIDRFKAKASKAKQA
                     QSRVKALARMERIAPVLTASDFAFEFKEPKSLPNPMLAMSNMECGYLVQDDVDPDTEP
                     TRKVIVRNINKTVLAGQRIGILGANGQGKSTLVKTIARSVPALGGEVTEGKGLSIGYF
                     AQQEMDVVHADDTPLQHMVRLARTVGPEGREQELRDFLGQFRFVGDMVHQVIGTLSGG
                     EKARLVLASLVWQRPNLLLMDEPTNHLDLTTREALSMALNEFEGTVMLVSHDRALLRE
                     VCDEFWLVAKGAVLPFDGDLDDYQKWLLETSRAVARGQPAPPLPGNLDSLVGGTPPPA
                     KAAKGPATPAAAPAPTPAATQTRPGAARNDDRKAAAQARTQAAAKTRPLRIEVEQIDK
                     RMEKLGAEKAEIEAAMSTGTMSGSEIADAGRRLAHVGAEIAMLEERWLELNEQIEALQ
                     AGT"
     gene            5513..6196
                     /locus_tag="VIN75_08540"
     CDS             5513..6196
                     /locus_tag="VIN75_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974251.1"
                     /translation="MTRLLRLLPLALCALLLEAAAAPEPPAPTYRSIGPDGKITYSDR
                     KPTDPGMRSRELGKTIPAPLVAPATGPFDLRPSSKPITERPVVGEGQTPAVDVSGRPF
                     PPGLPDAILTVLVHQFFVQSLVETCTRQRPAFVERYQGGVRNWRDRNADILARSNRVT
                     FARFTGEQRDTLRAIARQRLATVMPPPGASEAERATWCDRMSTDLATHQFELVGDMRI
                     APLLYVDVP"
     gene            6252..6893
                     /locus_tag="VIN75_08545"
     CDS             6252..6893
                     /locus_tag="VIN75_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974252.1"
                     /translation="MVTPQLPPAPQITDLIALSIRLVNANQPERARIVCDQALAMFEP
                     HPAVHQLLAVLDLQDGHVAAAREHAVRSLVLRPDHVPTLLVMGDIALAQHDLRAASQS
                     FERAVVLAPGLPEAWFKAALTRQDLHDLDGALAALERVLATSPERADALVNYGIVLQE
                     SGRLDDALRAYGRAFRLREQTFGRIAHALSTPGTGRLWLNLDDLRAELRALPA"
     gene            complement(6900..7184)
                     /locus_tag="VIN75_08550"
     CDS             complement(6900..7184)
                     /locus_tag="VIN75_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974253.1"
                     /translation="MIGRIVVLAGALACAGCAVLANVTPIGDGAYMAVVRSNDVNARV
                     QEQRSKAMSQAEAYCAERGAAVDVIKSVAEPPPPGQAPSAEVDFRCRGGS"
     gene            complement(7181..7891)
                     /locus_tag="VIN75_08555"
     CDS             complement(7181..7891)
                     /locus_tag="VIN75_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974254.1"
                     /translation="MKITFRCDPALIDLLPRPVPARTALPDWLRAMAPRVPSAVHGRE
                     IRTVKQCPPFVDAMRHGVLLVLPCDVKVERGMRLSWDWPLPALTVEGHPRAPLSFHVP
                     EQIAGSPLAHGQRSALKFNSFWTVELEPGWSLLAMHPFNRDDLPFRTVTGLVDADRFN
                     EVGINFPAVWLDEGFEGVLPKGLPFAQCCAVPREAPELRFEPMAPERVAGYNDVAARI
                     MAGPGVYRKGYRDKEGRG"
     gene            complement(8107..9840)
                     /locus_tag="VIN75_08560"
     CDS             complement(8107..9840)
                     /locus_tag="VIN75_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974255.1"
                     /translation="MKMRMNAVAFAAAALALGHGAWAGEAEAKKWIDNEFQPSTLTKD
                     QQMAEMKWFIDAAAKLKAKGVTQISVVSETLTTHEYESKTLAKAFEEITGIKVKHDII
                     QEGDVVEKLQTSMQSGKSIYDGWINDSDHIGTHYRYGTAIALTDAMAGPWKEYTNPGL
                     DLKDFIGTSFTTAPDGKMYQLPDQQFANLYWFRADLFARKDLQDKFKAKYGYDLGVPL
                     NWSAYEDIAEFFTNDVKTIDGKPIYGHMDYGKKDPSLGWRFTDAWLSMAGEADVGIPN
                     GLPVDEWGIRVADDKCTPVGASVERGGATNSPAAVYALTKYIDWMKKYAPKEATGMTF
                     GEAGPVPAQGQIAQQIFWYTAFTADMTKPGLPVVNADGSPKWRMAPGPNGPYWKQGMQ
                     NGYQDVGSWTFLKSNDPNRAAAAWLYAQFVTAKTTSLKKSITGLTFIRDSDIHSDYFT
                     KNANKYGGLIEFYRSPARVAWTPTGNNVPDYPKLAQLWWKNVAVAVTGEKTPQQAMDN
                     LASEMDDVMARLQRAGMAHCAPKLNPKSAPSKWLSTKGAPWAKLANEKPKGETIAYDT
                     LLNAWKAGKVR"
     gene            complement(9927..10235)
                     /locus_tag="VIN75_08565"
     CDS             complement(9927..10235)
                     /locus_tag="VIN75_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HEY8974256.1"
                     /translation="MFDWMAWTLPVAVFFSCIVLMLIGMTFWEVKSPTTLRRGWLPIA
                     TTRGDRLFIGLMAAAWVNLGFVAISEKLMTWFNLEAEPSIWWSFGLSMALLALVMRKG
                     "
     gene            complement(10352..>10744)
                     /locus_tag="VIN75_08570"
     CDS             complement(10352..>10744)
                     /locus_tag="VIN75_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8974257.1"
                     /translation="AHLLFNVPLAVWILEGFMSGIPREIDETAYIDGYSFPRFFLTIF
                     VPLIKAGIGVAAFFCFMFSWVELLMARTLTSVNAKPIVAVMTRTVSAAGMDWGTLAAA
                     GVLTIVPGAIVIWFVRNYIAKGFAMGRV"
BASE COUNT         1651 a         3885 c         3636 g         1572 t
ORIGIN      
        1 cggaaacatg gaacgcgtgc tgcatctgat ggccgacaag ggcgcatcgg atgtctacct
       61 gacggccaac tcgccgatcc agctgaagat caacggccag ctggtgccgg tgtccgacca
      121 gatcctcacg cccggccagc cccgcgcgct gctcgccgag ctgctgacgc ccgtgcaact
      181 ggaggaactc gaccagaccg gcgagctgaa cctgggcatc ggcattcgcg cggtgggcag
      241 cttccgcctg tccgcgttca agcagcgtgc gtcgatcgcc gccgtgatcc gctgcatccc
      301 gcacaagatc ccgtcgatgg acacgctgaa cctgccgccg gtgctggcgt cgctggcgct
      361 cgagcgccgc ggcctcgtgc tggtggtggg cgccaccggc aacggcaaga gcacgtcgct
      421 cgcgtcgatg atcgaatggc gcaacacgca gctgtcgggc cacatcctca ccatcgagga
      481 gccgatcgag ttcctgttct cgaacaagcg ctcgatcatc aaccagcgcg aagtgggccg
      541 cgacaccgcg tcgctgcaca tcgcgctgaa gaacgcgctg cgccaggcgc ccgactgcat
      601 cctgatcggc gagatccgcg atcgcgagac gatgtcgatg gccatctcgt acgcgctgtc
      661 cggccacctg gtgctggcca cgctgcacgc caacaacagc tcgcatacgc tgggccgcat
      721 tctgtcgttc tacacgccgg aagcgcggcc cgcgctgctg ggcgacctgg ccgcggcgct
      781 caaggccatc gtgtcgcagc gcctgctgcg ctcgacggcc ggcggccgcg tgccggcggt
      841 cgaggtgctg ctgaacagcc gcttcatcgc cgagctgatc gagaacggcg acatccaggg
      901 cgtgcgcgag gcgatggaca agtcgctggc ggaaggctcg cagatgttcg aggcggacat
      961 cgcgcgcctg atcggccagg gcctggtcac gcaggaagaa ggcctgctgg gcgccgactc
     1021 ccccaccaac ctgctgtggc gcctgcagaa cgagatgacg ccggtgtcgc gcgtgcagcc
     1081 ggccaaggtc gagtccgacg cgccgtcgtt caccgagatc acgctcgacg tgatccccga
     1141 ggaaccggcc ccgcgccagc gcctgggcgg cgtggccgtc gagatgcacc gctccaagca
     1201 cggttgacgc accgcattcc cgacccgtcg atggatgcca cgctccccct gctcgagcag
     1261 ctgatcgccc gtccctccgt cacgcccgac gacgcgggtt gccaggcgct gatcgcgcag
     1321 cggctgcagg ccatcgggtt cgcctgcgag cacctgccgt tcggccccgc cgacggcatc
     1381 gtcagcaacc tgtgggccgt gcgcgcaggc gcgcgcccgg gcccgacgct cgtcttcgcg
     1441 ggccacaccg acgtggtgcc caccggcccg cacgaccagt ggagcagcgc gcccttcgtg
     1501 ccgagccatc gcgaaggcaa gctgtacggc cgcggcaccg ccgacatgaa gacttcgctg
     1561 gccgcgatgg tcgtggccgc cgaggagttc gtcgcggcgc acccggatca cgccggccgc
     1621 gtcgcgttcc tgctcaccag cgacgaggaa ggcccgtcgg tggacggcac ggtgcgtgtc
     1681 gtcgacacgc tgcaggcgcg cggcgagaag ctcgactgct gcatcgtcgg cgagcccacc
     1741 tgcgtcgagc ggctcggcga catgatcaag aacggccgcc gcggcagcct ctcggcgcgc
     1801 ctgaccgtcg tcggcgtgca gggccacgtc gcctacccgc acctggcgcg caacccgatc
     1861 cacgtcgcgg cgccggccat cgccgagctc gccgccacgc gctgggacga cggcaacgac
     1921 cacttcccgc ccaccagctt ccagatctcc aacatccacg cgggcaccgg cgccaccaac
     1981 gtcatccccg gcgaggcggt gatcgacctg aacttccgct tctcgaccga gtcgacgccc
     2041 gagtccctga aggcgcgcgt cgtcgaggtc ctgcagcgcc acggcgtcga gcaccgcgtc
     2101 gcctggacgc tcggcggctc gcccttcctc acgcggccgg gcacgctcag cgaagcgctg
     2161 gtgcaggcca tcgtcgacga gaccggcggc gtccgcgcgg agctgtcgac gaccggaggc
     2221 acgtccgacg gccgcttcat ctcgcgcatt tgcccgcagg tggtggaatt cgggccgatc
     2281 aacgcgacga tccacaagat cgacgaacat gtggacgtgg ccgatgtgcc acggctcaag
     2341 aacatctacc ggcgcgcgat ggagaacctc ctcgtgccga acgcgacatg accctgatcg
     2401 actacctgca ccgggccggc gcccgcctcg acgaggcggg cgtttcgttt gggcacggca
     2461 ccaccaacgc gttcgacgag gccgcctggc tcgtgctgtg gaagctgggc ctgccgctgg
     2521 acgacctcga cggcgtcgca cggcgcgagc tcacggacgt cgagatcgcc acgctcgacg
     2581 ccttcgtcgg cgagcgcatc gccacgcgcc gcccggccgc gtatctcacg ggcgaggcct
     2641 ggctgcaggg cgtgccgttc acggtcgacg agcgcgtgat cgtcccgcgc tcgctgatcg
     2701 ccgaggtgct cgccgagggc gtcatcgacg cctggatgcc gccggacacg gcgcgcgtgc
     2761 tggacctgtg caccggcaac ggcagcctgg cggtgctcgc cgcgatggcc tggcccgacg
     2821 tcgccgtcga cgccgccgac ctgtccgtcg acgcgctcgc cgtcgccgcg ctcaacgtca
     2881 agcgccacga tctggcgggc cgcatcgcgc tgcaccgggg cgacggactt gccgcgctgc
     2941 ccgcgggccg gcgctacgac ctgatcctgt gcaatccgcc gtacgtcaac gcacagtcca
     3001 tgagcgagct gccggccgag tacaaggccg agcccgacat ggcgctggcc ggcggcgacg
     3061 acggcatgga cttcatccgc gcgatgttcg ccgccgcgcc cgaccgcatg accgacgacg
     3121 ccgtgctcgt gctggagatc ggcaacgagc gcgacttctt cgaggccgcc ttccccggcc
     3181 tcgaggtcgt ctggcttccc acgcaattgc atgacgacgc ggtgctcctg gtgacccgcg
     3241 ccatgctgga agacggcctt gcgccgcttc cgaaacacgg ttcctgaaca tgatcacgct
     3301 acgcaacgtg caactgcgcc gggggacgaa gatcgtcctg gacggcgtca acgtcaacgt
     3361 caatcccggc gagaaggtgg gcctggtcgg gcgcaacggc gccggcaagt ccagcctgtt
     3421 cgcgctgctg gccggccgcc tgcaggccga ccagggcgac ttcgagatcc cggcgcgctg
     3481 gcgcctgggc gaggtcgcgc agcacatgcc cgaaaccgag gactcggcca ccgacttcgt
     3541 gctcgaaggc gacatcccgc tgatggaggc ccagcgcgag ctggccgccg ccgaggccgc
     3601 cgaggacggc gaggcgatgg cgcacgcgca catgaagatc ggcgaggccg gcggcttcga
     3661 cgcgcgggct cgcgcacagg cgctgctgat gggcctgggc ttcaagaacg agcaggtcga
     3721 tgcgcccgtc aacagcttct ccggcggctg gcgcatgcga ctgcagctgg cccgcgcgct
     3781 catgtgcccg gccgacctgc tgctgctcga cgaaccgacc aaccacctgg acctcgatgc
     3841 cctcgtctgg ctcgagtcct ggctgcagcg cttcgacggc acgatcgtca tgatcagcca
     3901 cgaccgcgag ttcctcgacg ccatcaccgg cgtcacggtg cacctggacg aaggcacgct
     3961 gacgcgctac ggcggcaact actccacctt cgaggagatg cgggccgagc gcatgtcgca
     4021 gcaggccgcc gcccacagcc gccagcagga gaaggtggcg cagctgcagc gcttcatcga
     4081 ccgcttcaag gccaaggcca gcaaggccaa gcaggcgcag agccgcgtca aggcgctggc
     4141 gcgcatggag cgcatcgcgc cggtgctgac cgcgtcggac ttcgcgttcg agttcaagga
     4201 gccgaagagc ctgcccaacc cgatgctggc gatgtcgaac atggagtgcg gctacctggt
     4261 gcaggacgac gtcgatccgg acaccgagcc gacgcgcaag gtcatcgtcc gcaacatcaa
     4321 caagacggtg ctcgcgggcc agcgcatcgg catcctgggc gccaacggcc agggcaagtc
     4381 gacgctggtc aagacgatcg cgcgcagcgt gcccgcgctc ggcggcgagg tcaccgaggg
     4441 caagggcctg tcgatcggct acttcgcgca gcaggagatg gacgtcgtgc acgccgacga
     4501 caccccgctg cagcacatgg tgcgcctcgc caggaccgtg ggccccgagg gccgtgagca
     4561 ggagctgcgc gacttcctgg gccagttccg cttcgtcggc gacatggtgc accaggtgat
     4621 cggcacgctg tccggcggcg agaaggcgcg cctcgtgctc gcctcgctcg tgtggcagcg
     4681 ccccaacctg ctgctgatgg acgagccgac caaccacctg gacctcacca cccgcgaggc
     4741 gttgtcgatg gcgctcaacg agttcgaggg caccgtcatg ctggtcagcc acgaccgcgc
     4801 gctgctgcgc gaggtgtgcg acgagttctg gctggtcgcc aagggcgcgg tgctgccgtt
     4861 cgacggggac ctggacgact accagaagtg gctgctcgag acctcgcgcg ccgtggcccg
     4921 cggccagccc gccccgccgc tgccgggcaa cctcgacagc ctggtgggcg gcaccccgcc
     4981 gccggccaag gccgccaagg ggcccgccac gcccgcggcg gcgcccgcgc ccaccccggc
     5041 cgcgacgcag accaggcccg gcgccgcccg caacgacgac cgcaaggccg cggcgcaggc
     5101 ccgcacgcag gccgccgcga agacgcgccc gctgcgcatc gaggtcgagc agatcgacaa
     5161 gcgcatggag aagctcggcg ccgagaaggc cgagatcgag gcggcgatgt ccaccggcac
     5221 gatgtccggg tccgagatcg ccgacgccgg ccggcgcctg gcgcacgtgg gcgccgagat
     5281 cgcgatgctg gaggagcgtt ggctggagct caacgagcag atcgaggcgc tgcaggcggg
     5341 cacctgaaac tcggggggcg ccctggctcg cccgggcgcc gggctgtcgg aaggtggtac
     5401 cgtcgcggcg agacatcccg tgacctccgt cgcacgtgcg acacaggatg gcttccattc
     5461 cgtgcgccca ccagccgaca atcccgccat cccgtcctgg ccacgccaat cgatgacccg
     5521 actgctgcgc ctgcttcccc tcgcgctgtg cgccctcctg ctggaggccg ccgcggcgcc
     5581 cgagccgccc gcgcccacct accgcagcat cggcccggac ggcaagatca cgtactcgga
     5641 ccgcaagccg accgacccgg gcatgcgctc gcgcgagctc ggcaagacca tcccggcgcc
     5701 gctggtggcg ccggccaccg ggccgttcga cctgcgtccg tccagcaagc cgatcaccga
     5761 gcgccccgtc gtgggcgaag gccagacgcc cgcggtcgac gtcagcggcc ggcccttccc
     5821 gcccggcttg cccgacgcga tattgaccgt gctggtgcac cagttcttcg tgcagtcgct
     5881 cgtcgagacc tgcacccgcc agcgccccgc gttcgtcgag cgctaccagg gcggcgtgcg
     5941 caactggcgc gaccgcaacg ccgacatcct ggcccgcagc aaccgcgtca cgttcgcgcg
     6001 cttcaccggc gagcagcgcg acacgctgcg cgccatcgcg cgccagcggc tggccaccgt
     6061 gatgccgccg cccggcgcga gcgaggccga gcgcgccacc tggtgcgacc gcatgagcac
     6121 cgacctggcc actcaccagt tcgagctcgt cggggacatg cgcatcgcgc cgctgctgta
     6181 cgtcgacgtc ccctgagccg cggccgggcc acgagcgcga gtctcgtcgc cggagcgact
     6241 acgatgcacc catggtcacg ccgcagcttc cgccagcccc gcagatcacc gacctgatcg
     6301 ccctgtcgat ccggctcgtc aacgcgaacc agccggagcg ggcccgcatc gtgtgcgacc
     6361 aggcgctggc catgttcgag ccccatccgg cggtgcacca gctgctggcg gtgctggacc
     6421 tgcaggatgg ccacgtcgcc gccgcgcgcg agcacgcggt gcgcagcctc gtgctgcgcc
     6481 ccgaccacgt gcccacgctg ctcgtgatgg gcgacatcgc gctggcgcag cacgacctgc
     6541 gcgcggcgtc gcaatcgttc gaacgcgcgg tggtgctcgc gcccggcctg cccgaggcct
     6601 ggttcaaggc ggcgctgacg cgccaggacc tgcacgacct ggacggcgcc ctcgcggcgc
     6661 tggagcgggt gctggccacc agccccgagc gggccgacgc gctggtcaac tacggcatcg
     6721 tgctgcagga gagtggccgc ctcgacgacg ccctgcgcgc ttacggccgc gcgttccgcc
     6781 tgcgcgagca gaccttcggc cgcatcgcgc acgcgctgtc cacgcccggc accgggcggc
     6841 tctggctgaa cctggacgac ctgcgggccg agctgcgcgc cctgcccgcc tgaggcggct
     6901 caggacccgc cgcggcagcg gaagtcgact tcggccgatg gcgcctgtcc gggcggcggt
     6961 ggctcggcga ccgacttgat gacgtcgacc gcggcgccgc gttcggcgca gtacgcctcg
     7021 gcctgcgaca tcgccttgga ccgctgctcc tgcacgcgag cgttgacgtc gttgctgcgg
     7081 acgaccgcca tgtacgcgcc gtcgccgatg ggcgtgacgt tggcgaggac ggcgcagccg
     7141 gcgcaggcga gcgcaccggc caggacgacg atgcggccga tcatccgcgc ccttccttgt
     7201 cgcgataccc cttgcggtac acgcccggcc ccgccatgat gcgcgccgcg acgtcgttgt
     7261 agccggccac gcgctcgggc gccatcggct cgaagcggag ctccggcgcc tcgcgcggca
     7321 ccgcgcagca ctgcgcgaag ggcaggccct tgggcagcac gccttcgaag ccctcgtcca
     7381 gccacacggc gggaaagttg atgccgacct cgttgaagcg gtcggcgtcc accaggccgg
     7441 tgacggtgcg gaacggcagg tcgtcgcggt tgaacgggtg catcgcaagc agcgaccagc
     7501 ccggctccag ctcgaccgtc cagaagctgt tgaacttcag cgcggaacgc tggccgtgcg
     7561 ccagcggcga gcccgcgatc tgctcgggca cgtggaagct cagcggcgcg cgcgggtgcc
     7621 cctccaccgt caacgccggc agcggccagt cccagctcag gcgcatgccg cgctcgacct
     7681 tcacgtcgca cggcagcacc agcagcacgc catggcgcat cgcgtcgacg aacggcgggc
     7741 attgcttgac ggtgcggatc tcgcggccgt gcacggcgga cggcacacgc ggcgccatcg
     7801 cgcgcagcca gtcgggaagc gccgtgcgcg ccggcacggg gcgcggcagc aggtcgatca
     7861 gcgcggggtc gcagcgaaag gtgatcttca tcgggagcgg tcaatggcgg gcggccgggc
     7921 caaggggtct ggcctctcct tcatccgcgg ccaatgtgca ggccattgcg gcggcgctgc
     7981 ctgcggatga aggcgaaggc ctgccccctt tcgacatgcc gaagaataac gccccggacg
     8041 ccttgcggcg accggggcgc tcgagagtcc ggcccgccgg ccggacacgg agacaaccgt
     8101 tgcaggtcag cggaccttgc ccgccttcca cgcgttcagc agcgtgtcgt aggcgatggt
     8161 ctcgcccttg ggcttctcgt tggccagctt ggcccacggc gcgcccttgg tcgacagcca
     8221 cttgctcggc gcgctcttgg ggttgagctt gggcgcgcag tgcgccatcc cggcgcgctg
     8281 caggcgggcc atcacgtcgt ccatctcgct ggccaggttg tccatcgcct gctgcggcgt
     8341 cttctcgccg gtgacggcca cggccacgtt cttccaccac agctgggcga gcttgggata
     8401 gtcgggcacg ttgttgccgg tgggcgtcca ggccacgcgc gcgggactgc gatagaactc
     8461 gatcaggccg ccgtacttgt tggcgttctt ggtgaagtag tcggagtgga tgtccgagtc
     8521 gcggatgaac gtcaggccgg tgatcgactt cttgagcgac gtcgtcttgg ccgtcacgaa
     8581 ctgcgcgtag agccaggcgg ccgccgcgcg gttcgggtcg ttggacttca ggaacgtcca
     8641 cgagcccacg tcctggtagc cgttctgcat gccctgcttc cagtacgggc cgttcgggcc
     8701 gggggccatg cgccacttcg gcgaaccgtc ggcgttgacc accggcaggc ccggcttggt
     8761 catgtcggcg gtgaaggccg tgtaccagaa gatctgctgg gcgatctggc cctgggcggg
     8821 caccgggccg gcctcgccga aggtcatgcc cgtggcttcc ttgggcgcgt acttcttcat
     8881 ccagtcgatg tacttggtca gcgcgtagac ggccgccggc gagttggtcg ccccgccgcg
     8941 ttcgaccgac gcacccacgg gcgtgcactt gtcatccgcg acgcggatgc cccactcgtc
     9001 caccggcagg ccgttgggga tgccgacgtc ggcctcgccg gccatcgaca gccacgcgtc
     9061 ggtgaagcgc cagcccagcg acggatcctt cttgccgtag tccatgtggc cgtagatcgg
     9121 cttgccgtcg atggtcttga catcgttcgt gaagaactcg gcgatgtcct cgtacgcgct
     9181 ccagttcagc ggaacgccca ggtcgtagcc gtacttggcc ttgaacttgt cctgcaggtc
     9241 cttgcgcgcg aacaggtcgg cgcggaacca gtacaggttg gcgaactgct ggtcgggcag
     9301 ctggtacatc ttgccgtccg gcgccgtggt gaagctggtg ccgatgaagt ccttcaggtc
     9361 caggcccggg ttcgtgtact ccttccacgg gccggccatc gcatcggtca gcgcgatcgc
     9421 ggtgccgtag cggtagtgcg tgccgatgtg gtccgagtcg ttgatccagc cgtcgtagat
     9481 cgacttgccc gactgcatcg aggtctgcag cttctcgacg acgtcgcctt cctggatgat
     9541 gtcgtgcttg accttgatgc cggtgatctc ctcgaacgcc ttggcgagcg tcttggattc
     9601 gtattcgtgc gtggtcagcg tctcggacac caccgagatc tgcgtcacgc ccttggcctt
     9661 caacttggcg gcggcgtcga tgaaccactt catctcggcc atctgctgat ccttggtcag
     9721 cgtggacggc tggaactcgt tgtcgatcca cttcttggct tcggcctcgc ccgcccacgc
     9781 gccatggccg agagccaggg cggcagcggc aaaggccacg gcattcatgc gcatcttcat
     9841 gtgtgtctcc tgtgtgcctt cccgtcttct cccacggcac cggctgggaa ggattagccg
     9901 gtccctcgaa ctcgaatacc cggacttcaa cccttgcgca tgaccagcgc gagcagcgcc
     9961 atcgacaagc cgaagctcca ccagattgac ggctccgcct cgaggttgaa ccaggtcatc
    10021 aacttctcgc tgatcgcgac gaagccgagg ttgacccacg cggccgccat caggccgatg
    10081 aacaggcggt cgccgcgcgt cgtggcgatc ggcagccagc cgcggcgcag cgtcgtcggg
    10141 gacttgacct cccagaacgt catgccgatc agcatcagca cgatgcagct gaagaacacc
    10201 gcgacgggga gcgtccaggc catccaatcg aacatctctc atgcctccat atcgagcgaa
    10261 gagcagctcc caggccggcg cgcgcgcggc cgctccggag caagccggcc cagcccccac
    10321 ggggggcggc gagcgaagcg agtgggagtc gtcatactct gcccatcgcg aaacccttcg
    10381 cgatgtagtt gcgcacgaac cagatcacga tcgcgcccgg cacgatggtc agcacgccgg
    10441 ccgcggcgag cgtgccccag tccatgccgg ccgccgacac cgtgcgcgtc atcaccgcca
    10501 cgatgggctt ggcgttgacg ctggtcagcg tgcgcgccat cagcagctcg acccagctga
    10561 acatgaagca gaagaacgcg gccacgccga tgccggcctt gatcagcggc acgaagatcg
    10621 tcaggaagaa gcgcgggaac gagtagccgt cgatgtaggc cgtctcgtcg atctcgcgcg
    10681 ggatcccgct catgaagcct tccaggatcc acaccgccag cggcacgttg aacagcaggt
    10741 gcgc
//
LOCUS       DATCRO010000316         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_791080, whole genome shotgun sequence.
ACCESSION   DATCRO010000316 DATCRO010000000
VERSION     DATCRO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791080"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VIN75_08575"
     CDS             <1..406
                     /locus_tag="VIN75_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974258.1"
                     /translation="ITGGFLAAHAEIVDQKLNVTGGVLDWIGVPPVGQTDAAGNPLVA
                     VYYLVTLMQAGPDDHRKPYRMTTEAVEVDGSSRVLIDATIAVDAHSGENRFWVTAFGM
                     AGTTSGRRVLVQTIEGGGSIALPVEVRVVEPL"
     gene            472..2037
                     /gene="lnt"
                     /locus_tag="VIN75_08580"
     CDS             472..2037
                     /gene="lnt"
                     /locus_tag="VIN75_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8974259.1"
                     /translation="MTVGGVSVVAGLLLWASFPVLDWWFAAIAAFALLAWVLITPATT
                     ALGGLACGVVFGLACYLPLLRWSGDFVGVWPWLVLAAGCSLYPGVFGVCAVLVRDLPG
                     WPIWFAALWAAQEWVKARVPFGGFPWGEVGTGQSTGLLLPVAAVGGVGLLSATVVLAG
                     CTVTACSLRLARRVGAAVGASRHGRHAAVSMWLPGLCLGLIASATVVVWMQLGDGRAG
                     PTVQVAVVQGNVPRLGLDFNAQRRAVLDFHVRETLRLAADVAAGRARPPRLVIWPENS
                     SDIDPLTNHDAAQEIAAAAAAINAPILLGGVRAGPGWSREHPRWLNSVMVFDPRNGWG
                     PRHDKAIIQPFGEYLPWPGLFGRLPYARHVSHFVPGAGPGVVHAAGVAVGVATCWEIL
                     FDRAPRGAVLAGAQLLAVPTNNATFDATMSHQQLAFAAVRAVEHHRYVLVAGTTGISA
                     VIAPDGHPIARTGFNTSAYLDEPVHFNAVMTPATQWAPAIEWLLIGIGVTALAAATLT
                     HRHATTPRHAAIV"
     gene            complement(2972..>3367)
                     /locus_tag="VIN75_08585"
     CDS             complement(2972..>3367)
                     /locus_tag="VIN75_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974260.1"
                     /translation="LQGGATYTAAGGAGGASSETTAATVGAAPGDISFDGHSYFGGWA
                     GAAGYNSAGGNATAPGAAGGGGGSYGSLGGAGGASGAYFTITVPITSGMTEITGNVGA
                     GGTGGAAINTAVGGNGAPGAAWFVFYSEE"
BASE COUNT          545 a         1053 c         1122 g          647 t
ORIGIN      
        1 gatcaccgga gggttcctag cagcacacgc cgaaatcgtc gaccagaaac tcaacgtcac
       61 cggcggagtg ctcgactgga ttggcgtccc cccggtcggc caaacagacg ctgccggaaa
      121 cccgctcgtg gccgtctact atctggtcac actgatgcaa gccggtccgg acgatcatcg
      181 aaagccttac cgcatgacga ctgaggccgt cgaggtcgac gggagcagcc gcgtcctcat
      241 cgacgcaacg atcgccgtcg atgcccactc gggcgaaaac cgcttttggg taacagcttt
      301 cggcatggcc ggcacaacca gcggccgcag agtgctggtc caaaccatcg aaggcggcgg
      361 atccattgct ctccccgtcg aagtgcgcgt tgttgaacca ctgtgacttc ggtatctgcc
      421 aacgttaatc cgtttgcctt gaggcactgg ggtgttgcac cggcccgccg gttgacggtc
      481 ggcggtgtct cggtagttgc cggattgttg ttgtgggcca gcttccccgt gttggactgg
      541 tggtttgcag cgatcgcggc gttcgcgttg ttggcgtggg tgctgatcac gccggcgacc
      601 acagcgctgg gcgggctggc ctgcggtgtc gtgttcgggc tcgcgtgcta cctgccgctg
      661 ttgcggtgga gcggtgattt cgtgggggta tggccgtggt tggtgttggc ggcggggtgt
      721 tctctgtatc caggcgtgtt cggggtgtgc gcggtgttgg tgcgtgacct gccaggctgg
      781 ccgatctggt tcgcggcgct ttgggctgcc caggaatggg tcaaggcgcg tgttcctttc
      841 ggcgggtttc cctggggtga ggtcgggacc ggtcaaagca caggactgct gttaccggtg
      901 gccgcggtcg gtggggtggg cttgctgtcg gcgacggtcg tgctggcggg gtgcacggtg
      961 acggcgtgct cgctgagact ggcgcgtcgc gtaggcgccg cggtcggggc tagtcgccac
     1021 ggcaggcatg cggcggtgtc gatgtggctg ccggggttgt gtctggggtt gatcgcgtcg
     1081 gcgaccgttg tggtgtggat gcagctgggt gacggtcggg cgggcccgac ggttcaggtg
     1141 gccgtggtgc aaggcaatgt gccgcgtctg gggttggatt tcaacgctca acgccgtgcg
     1201 gtactcgact tccacgtgcg tgaaaccctg cggttggctg ccgatgtcgc ggccgggcgg
     1261 gcccgcccgc cacggctggt gatctggccg gagaactcct cagatatcga tccgttgacc
     1321 aaccacgacg ccgcgcaaga gattgcggcc gcggccgcgg cgattaacgc gccaatcctg
     1381 cttggcgggg ttcgcgccgg gccgggctgg agccgtgagc atccgcgctg gttgaactcg
     1441 gtgatggtgt tcgatccgcg caacgggtgg ggtcctcgcc acgacaaggc gataatccag
     1501 ccgttcgggg agtacctacc ctggccaggc ctgttcggac gcctgccgta tgccaggcac
     1561 gtgagtcatt tcgttcccgg tgcgggcccc ggtgtggtgc acgccgccgg tgtggcggtt
     1621 ggggtggcga cctgctggga gatcctcttc gatcgtgcgc cgcgcggtgc ggtgctcgcc
     1681 ggggcgcaac tgctggccgt gccgactaac aacgccacct tcgacgccac gatgagccat
     1741 caacaattgg ccttcgccgc cgtacgggcc gtcgagcatc accgttacgt gctggtggcc
     1801 ggtaccaccg gcatcagcgc cgtaatcgct cctgatggcc acccgatcgc gcgcaccggc
     1861 ttcaacacat cggcctacct cgatgaaccg gtgcatttca acgccgtcat gacccccgcc
     1921 acccagtggg cacccgccat cgagtggctc ctgatcggca tcggtgtcac agctctcgcc
     1981 gccgccaccc tcacacaccg acacgcaacc accccacgac acgcggcgat cgtctgaggc
     2041 tcaaattcgt ccacggcaca aagagattcg atagccgtaa gccacgacga gcgggtgcgg
     2101 cactcatatg ccacgcctaa cttctccagc actgccgccc ggcatacgac tgaaaacaga
     2161 attaattaac tgttatttgg gaactattac cgggacaaag gggacaggag cggagtggtg
     2221 ttccggaggg tgacaagccg ggtccgggga atccggtgtg caccctggcg gcaacggcat
     2281 ttccggacca gggtcatgtc cgccggtcat ccaaaccacc gaatccacaa cgccctgccc
     2341 agccattaca ccaggggcag cgccagatgg gccggcactt gtcgtgcctg ccaccagcaa
     2401 cacgcccgct acacacgtcg caactctgct gcgaccgatt ccacgcgaca ccaacaacac
     2461 cgccttcgtc accaacatgg cgcaccgaaa tgcgcagcgg actagagcac ttcagtaact
     2521 gcgtacaccc gcgattcgtc gataataata atttatttgg ctgggatcag gctgtcacta
     2581 tcccgcacaa ccactgccag caggatgcga attttcgatc gtgcgcggcg cggtattcgt
     2641 gttaggtcgg tacctggtgt gccgctccgt tgtggcgacg atctggtcga gccagtatac
     2701 cgacaacgca aggccagaga ctggcgcaca cccggatcaa tcaatggacg tcaggctact
     2761 cctgaatcgg aggtgggcgt aggcatgtct tttgtagccg cctaccatta gtgctggaat
     2821 atcaccggct tgcgttcccc gcatgcatat ttcagtctga gaccgtcggc gttgagcaac
     2881 gatcggcgca gacgcctgct ggcagcagct aagcgaaagc cgccccggtc tgccccgggg
     2941 cggcttcgaa tggctgaggc cgatgggccc cttactcctc gctgtagaag acgaaccatg
     3001 cagcgcccgg agcgccgttg cctccgacgg cggtgttgat ggcagcaccg ccggttccgc
     3061 ctgcgccgac gttgccggtg atctcagtca tgccggaggt gatgggaact gtgatcgtga
     3121 aatatgcgcc tgaggctccg cctgcaccgc cgaggctgcc gtatgagccg cccccgccgc
     3181 ctgccgcgcc gggcgctgtc gcgtttccgc ccgcgctgtt gtagccggcg gcgccggccc
     3241 agcctccgaa gtagctgtga ccgtcgaagg atatgtcgcc gggcgctgcg cctacggtgg
     3301 cggcagtggt ctcgctactg gcgccgccag cgccgccggc ggcggtgtaa gtggcacctc
     3361 cttgaag
//
LOCUS       DATCRO010000317         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_554843, whole genome shotgun sequence.
ACCESSION   DATCRO010000317 DATCRO010000000
VERSION     DATCRO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554843"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..870)
                     /gene="panC"
                     /locus_tag="VIN75_08590"
     CDS             complement(16..870)
                     /gene="panC"
                     /locus_tag="VIN75_08590"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8974261.1"
                     /translation="MQVIHTLADLRAVLAGSSKTALVPTMGNLHEGHLALVREAALRG
                     FPVVATIFVNRLQFLPHEDFDQYPRTLERDCQLLEGAGCDIVFAPSESELYPEPQTFK
                     VTPPAAIGDLLEGEFRPGFFTGVCTVVMKLFQCVQPAVAVFGQKDYQQLMVIKAMARQ
                     FALPIEIIGAPTVRANDGLALSSRNGYLSQDERAEATALARALRAIAAALREGARDVA
                     AVEARQKAELDARGWKTDYLTVRRRADLLAPTPEDLAAKTPLVALAASRLGKTRLIDN
                     LEIDLQDT"
     gene            complement(906..1751)
                     /gene="panB"
                     /locus_tag="VIN75_08595"
     CDS             complement(906..1751)
                     /gene="panB"
                     /locus_tag="VIN75_08595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8974262.1"
                     /translation="MSAHSSPTSSAAQAARAAVTLPKLREMRARGEKVAMLTCYDATF
                     AQLLDEAGVDTLLVGDSLGNVMQGRTTTLPVTLEHMVYHTECVARVQPAAWLIADMPF
                     GSYENGKGPALDSAVALMRAGARMVKLEGGGWTAEVVSHLVARGIPVCAHLGLTPQRV
                     HALGGYRVQGREGDSAAALHRDARELAEAGAAMLVLEMVPAALATEITSENPELITIG
                     IGAGGGTSGQVLVLHDMLGLGGGRKPRFVRNFMAEGGSIASAIGRYVAAVKDGSFPQA
                     DVHTY"
     gene            complement(1894..3384)
                     /locus_tag="VIN75_08600"
     CDS             complement(1894..3384)
                     /locus_tag="VIN75_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY8974263.1"
                     /translation="MNAVFAILAVQALLGGFDNLWHHELHAKLPRRRSARRELALHAA
                     REAIYAVVFLALAWTEWHGALALLLATLLGVEVVITLADFLEEDRSRRLPPFERVLHT
                     VLALTYGLFLAALAPHLADWTRRPTGIAWTPHGLVSLAFSACAAGLAAWSVRNALAVR
                     ALGRVPEAAAPAPRHDGRIVPAVLVTGATGFVGSALVEQLRRDGRRVIVLARDLLQAR
                     GLFGDDARVVDRLDDIPSETVIDAVVNLAGARVLGMPWTRARRAELLRSRTATTAAVV
                     DLMRRLQQRPRVLVSASAVGFYGATPQGAFDPCDERSPPRPGQFQSDLCAAIEHEARR
                     AEALGVRVVRMRLGVVLGRGDGAYPMLALSARLGFGAVLGSGRQPAPWIHLDDAVGLI
                     RFALADGGLAGPVNAVAPGTPSQEAFMRALAASFGRGVHLRMPHGVLRRALGEMAELL
                     LEGQNAVPAAALAAGYRYRHPVLEEALAELAGRSHRRAGQPGPAAL"
     gene            complement(3381..>3878)
                     /locus_tag="VIN75_08605"
     CDS             complement(3381..>3878)
                     /locus_tag="VIN75_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEY8974264.1"
                     /translation="LRCSPAGRVFAALAKAFGSPLSGATDAAVPTSVRVLDDGRGGMV
                     WERSFHTNAAGAPHVVRSTKELDAGGRLVERTDGGLGMALRVFEEQGALVFESHRFFL
                     VLGRLRLPVPALLTPGTCRVVHTDLGGGLFRFDLDMVHPLWGRTFHQSGVFADPVAAS
                     EGSAR"
BASE COUNT          534 a         1413 c         1451 g          480 t
ORIGIN      
        1 gtcggagccc ggcccttacg tgtcctgcag gtcgatctcg aggttgtcga tcagccgcgt
       61 cttgcccagg cgcgacgcgg cgagcgcgac cagcggggtc ttggcggcaa ggtcctcggg
      121 cgtgggcgcc agcaggtccg cgcggcggcg caccgtcagg tagtcggtct tccagccgcg
      181 cgcgtcgagc tcggctttct gcctggcctc gacggccgcg acgtcgcgcg cgccctcgcg
      241 cagcgcggcc gcgatcgcgc ggagcgcgcg cgcgagcgcc gtggcctcgg cgcgctcgtc
      301 ctggctcagg tagccgttgc gcgaggacag cgccaggcca tcgttggcgc gcacggtggg
      361 cgcgccaatg atctcgatgg gcagcgcgaa ctggcgcgcc atcgccttga tgaccatcag
      421 ctgctggtag tccttctggc cgaacacggc gacggccggc tgcacgcact ggaacagctt
      481 catgacgacg gtgcacacgc cggtgaagaa gccggggcgg aactcgcctt cgagcaggtc
      541 gccgatggcc gcgggcggcg tcaccttgaa ggtctgcggc tcggggtaga gctcggattc
      601 gctcggcgcg aacacgatgt cgcagcccgc gccctcgagc agctggcagt cgcgctccag
      661 cgtgcgcggg tactggtcga agtcctcgtg cggcaggaac tgcaggcggt tgacgaagat
      721 cgtggcgacg acggggaaac cgcgcagcgc ggcctcgcgc accagcgcca ggtggccctc
      781 gtgcaggttg cccatcgtcg gcacgagggc cgtcttgctc gagccggcga gcacggcgcg
      841 caggtcggcg agggtgtgga tgacttgcat ggtggcttcc agtgtgccgc gtggcccggt
      901 cgggatcagt aggtgtggac gtccgcctgc gggaagctgc cgtccttgac cgcggcgacg
      961 tagcgcccga tggccgaggc gatcgagccg ccctcggcca tgaagttgcg cacgaagcgc
     1021 ggcttgcggc cgccgcccag gcccagcatg tcgtgcagca ccagcacctg gccggacgtg
     1081 ccgccgcccg cgccgatgcc gatcgtgatg agctcggggt tctcgctggt gatctcggtg
     1141 gcgagcgcgg ccggcaccat ctcgagcacc agcatcgcgg cgccggcctc ggccagctcg
     1201 cgcgcgtcgc ggtgcagtgc ggcggcgctg tcgccctcgc ggccctgcac gcggtagccg
     1261 cccagcgcgt gcacgcgctg cggtgtcagg cccaggtggg cgcacacggg gatgccccgc
     1321 gcgaccaggt gcgagacgac ctcggccgtc cagccgccgc cttcgagctt gaccatgcgc
     1381 gcgccggcgc gcatcagcgc cacggccgaa tcgagcgccg ggcccttgcc gttctcgtag
     1441 ctgccgaacg gcatgtcggc gatcagccag gccgccggct gcacgcgggc gacgcactcg
     1501 gtgtgataga ccatgtgctc cagcgtcacg ggcagcgtgg tggtgcgccc ctgcatgacg
     1561 ttgcccagcg aatcgcccac cagcagcgtg tcgacgccgg cttcgtcgag cagttgcgcg
     1621 aaggtggcgt cgtagcaggt gagcatggcc accttctcgc cgcgggcgcg catctcgcgc
     1681 agcttgggca gcgtgacggc ggcgcgggcg gcctgggcgg ccgacgaggt ggggctggaa
     1741 tgggcgctca tgctgatgtc ctgtggggag acgggtagct gcctggactc cggcgtcccc
     1801 ccggtcaggg gtgcccggat gcggcgcgag gcgcattgtg cacaggtccg cgggcacttg
     1861 tgcggcggtg cacaaaaagt tgggcgaatc gctctaaagc gccgcgggac cgggctgccc
     1921 ggcacgtcga tgcgagcgtc cggccagttc ggccagcgcc tcttccagca cggggtgacg
     1981 gtagcggtag cccgccgcca gggcggccgc cggcaccgca ttctggccct ccagcagcag
     2041 ttcggccatc tcgccgagcg cccgccgcag gacaccatgc ggcatcctca ggtgcacgcc
     2101 gcgaccgaac gacgccgcca gcgcgcgcat gaacgcctcc tggctgggcg tgcccggcgc
     2161 caccgcgttg accggtccgg ccaggccccc gtcggccagc gcgaagcgga tcaggccgac
     2221 cgcgtcgtcc aggtggatcc acggcgccgg ttggcgcccc gagcccagca ccgcgccgaa
     2281 ccccagccgc gccgacagcg ccagcatcgg gtacgcgccg tcgccgcgcc ccagcacgac
     2341 gcccaggcgc atgcgcacga cgcgcacgcc gagcgcctcc gcgcgccgcg cctcgtgctc
     2401 gatcgcggcg cacaggtcgg actggaactg gcccggccgc ggggggctgc gctcgtcgca
     2461 ggggtcgaac gcgccctgcg gcgtcgcgcc gtagaaaccg accgccgacg cgctgaccag
     2521 cacgcgcggc cgctgctgca accggcgcat cagatcgacg acggccgccg tcgtcgcggt
     2581 gcggctgcgc agcagctcgg cgcggcgggc gcgggtccat ggcatgccca gcacgcgcgc
     2641 gccggccagg ttgacgacgg cgtcgatcac ggtctcggac gggatgtcgt cgaggcggtc
     2701 gaccacccgg gcgtcgtcgc cgaacagccc gcgcgcctgc agcaggtcgc gggcgagcac
     2761 gatgacgcgc cggccgtcgc gccgcaattg ctcgaccagc gccgagccga cgaagccggt
     2821 cgcgcccgtc accagcacgg ccggcacgat gcggccatcg tggcgcggtg cgggagcggc
     2881 ggcctcgggg acgcggccca gcgcgcgcac ggccagcgcg ttgcgcacgc tccacgcggc
     2941 caggcccgcc gcgcatgcgc tgaacgccag cgagacgagg ccatgcggcg tccacgcgat
     3001 gccggtcggc cggcgcgtcc agtcggccag atgcggcgcg agcgcggcca ggaacaggcc
     3061 gtaggtcagc gccagcaccg tgtgcagcac gcgctcgaac ggcggcagcc ggcggctgcg
     3121 gtcctcttcc aggaagtccg ccagcgtgat caccacctcc acgcccagca gcgtggccag
     3181 cagcagcgcg agcgcgccgt gccactccgt ccacgcgagc gccaggaaga cgactgcgta
     3241 gatcgcctcg cgcgccgcgt gcagcgccag ctcgcggcgt gccgagcggc gccgcggcag
     3301 cttcgcgtgc agctcgtggt gccagaggtt gtcgaagccg ccgagcagcg cctgcacggc
     3361 caggatcgcg aagacggcgt tcatcgtgcg ctcccttcgg aagcggcgac cgggtcggcg
     3421 aacacgcccg actggtggaa ggtgcggccc cacagcgggt gcaccatgtc cagatcgaag
     3481 cggaacaggc cgccgcccag gtcggtgtgc acgacgcggc aggtgccggg cgtcagcagc
     3541 gcgggcaccg gcaggcgcag ccggcccagc acgaggaaga agcggtggct ctcgaacacg
     3601 agggcgccct gctcctcgaa cacgcgcagc gccatgccga gaccgccgtc ggtgcgctcg
     3661 accagccggc cgccggcgtc cagttccttg gtggaacgca cgacgtgcgg cgcgccggcc
     3721 gcgttcgtgt ggaagctgcg ctcccagacc atgccgccgc ggccgtcgtc cagcacgcgc
     3781 accgacgtgg gcacggcggc gtcggtcgcg cccgacaacg ggctgccgaa ggccttggcg
     3841 agcgcggcga acacgcggcc cgccggcgag cagcgcag
//
LOCUS       DATCRO010000318         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_374499, whole genome shotgun sequence.
ACCESSION   DATCRO010000318 DATCRO010000000
VERSION     DATCRO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374499"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VIN75_08610"
     CDS             <1..848
                     /locus_tag="VIN75_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974265.1"
                     /translation="GAAAREATVAASAAAAAASARANVDISDVDPLAPRYKEWDALAA
                     REHAQARPVAATLPFGADRLGRDVLSKALKGTQISVSVGVLAAIVATLIGASLGAVAG
                     FFGGRVGDLVEWVYNVLTAVPEILLFFAFAAVFGRGISTLVLIMGLCGWTGQYRQVRA
                     EFIKHARREYVRAAEAIGAGAGARMFRHILPNVSHVILVRMSLMVVGFIKAEVILSYL
                     GLGVATDQVSWGTMLAESQSELILGHWWQLAAATIAMAVFVTAFSLMADSWRDALDPR
                     IRGQR"
     gene            1006..2775
                     /locus_tag="VIN75_08615"
     CDS             1006..2775
                     /locus_tag="VIN75_08615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8974266.1"
                     /translation="MTSLLKVEDLHVAFRLHDERGRSRHVEAVGRGAQGISFEVPANT
                     NVALVGESGSGKSVTAMSLVQLLPRNAECAGRILWKGEDLLQSTRARMQRLRGREIAC
                     VFQDPMSSLNPVLSVGEQVAEPLRKHLRMSRAVARARAEELLAEVGIADPRRRLDAYP
                     HQLSGGQQQRVMIAMALACEPQLLIADEPTTALDVTIQRQILELLARLQAQRGLSMLF
                     ISHDLSLVGEIAHQIVVMRQGTVRERGSVADLFGAPKDAYTKALLACRPRLDDKPRRL
                     TVIDDVLVVEAAASGASKAQAEPAAPARSVDVDLNPRRADDDDPVVLQVDGLSKSFWL
                     RSGLFARAEFKAVQDVRFTLRRGHTLGVVGESGSGKTTMGLTLLRLHDATSGPLSGRV
                     LFHGHDLLALAPADRLAMRRRIQIVFQNPYEALNPRFTIGQALVEPMTIHGIGVDAPA
                     RERKARDWLVRVGLDADAMHKYPHEFSGGQRQRIAIARSLTLSPDVLVLDEAVAALDV
                     SVQAQVLNLLKDLQDELGLAYVFISHDLAVVRFMADDVMVMKDGRVVEHRRTDALLAY
                     PQDDYTRRLIGAVTRAPAAGAPA"
     gene            complement(2779..3816)
                     /locus_tag="VIN75_08620"
     CDS             complement(2779..3816)
                     /locus_tag="VIN75_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8974267.1"
                     /translation="MQSLKVLFALTAASALVACGGGGGDSPSPAPVPAPAPSPGVGSN
                     GTLQTSVPAAAYADNRHLSAFNQLNAVRLGAGAGLLAQSTALDTSAQDHADYLTTNGY
                     NSADSPHDENAADPGFTGANPFVRMTNAGYDWGYATEVIGDIGSSSSTSDCVGDLLDT
                     VYHAVSMLSRATDVGFGYGTGGAAGMCTIDMASPLNGGDAKQIPPSGAIVAYPFDGST
                     VAHGTFRVSAETPRVSLSLLPNATAGTPIILGFRNQDVVAGGKVTISQLTLATSTGTA
                     VPTVILGGANVSGTNVTADATLGTDYPDFAVLVSTSPLAAGSYTVTLHASISGGRALA
                     LNTWTFNVAAP"
BASE COUNT          538 a         1383 c         1513 g          584 t
ORIGIN      
        1 cgggtgcggc cgcgcgcgag gcgacggtcg ccgcgtcggc cgccgccgcg gcggcctcgg
       61 cgcgggccaa cgtcgacatc tcggacgtcg acccgctggc gccgcgctac aaggaatggg
      121 acgcgctggc cgcccgcgag cacgcgcagg caaggccggt ggcggccacg ctgcccttcg
      181 gcgccgaccg gctcgggcgc gacgtgctgt ccaaggcgct caagggcacg cagatctcgg
      241 tgtcggtggg cgtgctggcg gcgatcgtgg ccacgctgat cggcgcctcg ctgggcgcgg
      301 tggccggctt cttcgggggg cgcgtcggcg acctcgtcga atgggtctac aacgtgctga
      361 ccgcggtgcc cgagatcctg ctgttcttcg cgttcgcggc ggtgttcggg cgcggcatct
      421 ccacgctggt gctgatcatg ggcctgtgcg gctggaccgg ccagtaccgc caggtgcgcg
      481 ccgagttcat caagcacgcg cggcgcgaat acgtgcgcgc cgccgaggcc atcggcgccg
      541 gcgccggcgc gcgcatgttc cgccacatcc tgcccaacgt gagccacgtg atcctggtgc
      601 gcatgtcgct gatggtggtg ggcttcatca aggccgaggt catcctgtcg tacctgggcc
      661 tgggcgtggc caccgaccag gtcagctggg gcacgatgct ggccgagtcg cagagcgagc
      721 tgatcctcgg gcactggtgg cagctggctg cggcgacgat cgcgatggcg gtgttcgtca
      781 ccgcgttctc gctgatggcc gactcctggc gcgacgcgct cgacccgcgc atccgcggac
      841 aaagatgaca gccccctcgc tcctgccgtc gggaagcatc cggctcgcag gccggatgcc
      901 gttccgtcgg ggccttgctg cgcaagtcct ttgccgcctt cactccccca agggggagct
      961 cggttcggct cgggtggtga ccctcgcttc ccgaggacgc ttcgcatgac gtcgctcctg
     1021 aaggtcgagg acctgcacgt cgccttccgc cttcacgacg agcgcggccg ctcccgccat
     1081 gtcgaggccg tgggccgcgg cgcccagggc atcagcttcg aggtgccggc caacaccaac
     1141 gtggcgctgg tgggcgagtc gggctcgggc aagagcgtca cggcgatgtc gctcgtgcag
     1201 ctgctgccgc gcaacgccga gtgcgcgggg cgcatcttgt ggaagggcga ggacctgctg
     1261 cagtcgacgc gcgcgcgcat gcagcgcctg cgcggacgcg agatcgcctg cgtgttccag
     1321 gatccgatgt cctcgctcaa cccggtgctg agcgtgggcg agcaggtcgc cgagccgctg
     1381 cgcaagcacc tgcgcatgag ccgcgccgtc gcgcgggcgc gggccgagga actgctggcc
     1441 gaggtgggca tcgccgaccc gcggcggcgg ctcgatgcgt atccgcacca gctctcgggc
     1501 gggcagcagc agcgcgtcat gatcgcgatg gcgctcgcgt gcgaaccgca gctgctgatc
     1561 gccgacgagc ccaccacggc gctggacgtc acgatccagc gccagatcct ggagctgctc
     1621 gcgcgcctgc aggcccagcg cggactgtcg atgctgttca tcagccacga cctgtcgctg
     1681 gtgggcgaga tcgcgcacca gatcgtcgtg atgcgccagg gcacggtgcg cgagcgcggc
     1741 tccgtggccg acctgttcgg cgcgcccaag gacgcctaca ccaaggcgct gctcgcctgc
     1801 cgcccgcgcc tggacgacaa gccgcgccgg ctcaccgtca tcgacgacgt tctggtcgtc
     1861 gaagcggcgg ccagcggcgc gtcgaaggcc caagccgagc ccgccgcgcc ggcgcgcagc
     1921 gtcgacgtcg acctgaaccc gcgccgcgcc gacgacgacg atccggtggt gctgcaggtc
     1981 gacggcctgt ccaagagctt ctggctgcgc agcggcctgt tcgcgcgcgc cgagttcaag
     2041 gcggtgcagg acgtgcgctt cacgctgcgc cgcggccaca cgctgggcgt ggtgggcgag
     2101 tcgggctcgg gcaagacgac gatgggcctg acgctgctgc gcctgcacga cgccacgagc
     2161 ggcccgctgt cgggccgcgt gctgttccac ggccacgacc tgctcgcgct cgcgccggcc
     2221 gaccgcctgg cgatgcggcg ccgcatccag atcgtcttcc agaacccgta cgaggcgctg
     2281 aacccgcgct tcacgatcgg gcaggcgctg gtcgagccga tgacgatcca cggcatcggc
     2341 gtcgacgcgc cggcgcgtga acgcaaggcc cgtgactggc tggtgcgcgt gggcctggac
     2401 gccgacgcga tgcacaagta cccgcacgag ttctcgggcg gccagcgcca gcgcatcgcc
     2461 atcgcgcgca gcctcacgct gtcgcccgat gtgctggtgc tggacgaggc ggtcgccgcg
     2521 ctcgacgtgt cggtgcaggc gcaggtgctc aacctgctga aggacctgca ggacgagctg
     2581 ggcctggcct acgtgttcat cagccacgac ctggccgtcg tgcgtttcat ggccgacgac
     2641 gtgatggtga tgaaggacgg ccgcgtcgtc gagcaccgcc gcaccgacgc gctgctggcg
     2701 tatccgcagg acgactacac gcgccgcctg atcggcgcgg tgacgcgcgc gcccgccgca
     2761 ggcgcgccgg cctgagcgtc agggcgccgc cacgttgaac gtccaggtgt tcagcgcgag
     2821 cgcccggccg ccgctgatcg aggcgtgcag cgtgacggtg tagctgccgg cggccagcgg
     2881 gctggtggaa accagcacgg cgaagtcggg gtagtcggtg cccagcgtgg cgtcggcagt
     2941 gacgttggtg ccggagacgt tcgcgccgcc gaggatcacc gtcggcaccg ccgtgcccgt
     3001 cgaggtcgcc agggtcagct gcgagatcgt caccttgccg cccgcgacca cgtcctggtt
     3061 gcggaagccg aggatgatgg gggtaccggc cgtggcattg ggcagcagcg acagggacac
     3121 ccgcggggtc tcggcggaca cgcggaaggt cccgtgcgcc acggtcgagc cgtcgaacgg
     3181 atacgccacg atcgcgcccg acggcgggat ctgcttcgca tcgccgccgt tcagcggcga
     3241 ggccatgtcg atggtgcaca tgccggccgc gccgcccgtg ccgtagccga agccgacgtc
     3301 ggtggctcgg ctgagcatcg agacggcgtg gtagaccgtg tcgagcaggt cgcccacgca
     3361 gtcgctggtg gacgaggacg agccgatgtc gccgatcacc tcggtggcgt agccccagtc
     3421 gtagccggcg ttggtcatgc gcacgaaggg attggcgccg gtgaagcccg ggtcggcggc
     3481 gttctcgtcg tggggggaat cggcgctgtt gtagccgttg gtggtgaggt agtcggcgtg
     3541 gtcctgggcc gaggtgtcga gcgcggtgga ctgcgccagc agcccggcgc ccgcgcccag
     3601 gcgcacggcg ttgagctggt tgaacgcgct caggtggcgg ttgtcggcgt aggccgcggc
     3661 ggggacgctc gtctgcagcg tgccgttgct gccgacgccc ggggacggcg cgggtgccgg
     3721 gacgggggcg ggcgacggcg aatcgccgcc cccaccgccg caggcgacga gggccgaggc
     3781 cgcggtcagg gcgaacagga ctttgaggga ctgcatcggg agcgggatgg tgagcggcaa
     3841 aacgggcggg cggggcccgc gctcgcgcgc ccggccccgc ccaggggttc cattttcgac
     3901 ggcgcggctg cgcgcccgcg cggcatttac cggaggtttc caattcgctg ggcggtgcgg
     3961 cgcacggaac tgttcgccac ggtcgagatc catccaccac ctcgaccaac acggagac
//
LOCUS       DATCRO010000319         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_149844, whole genome shotgun sequence.
ACCESSION   DATCRO010000319 DATCRO010000000
VERSION     DATCRO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149844"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VIN75_08625"
     CDS             <1..607
                     /locus_tag="VIN75_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974268.1"
                     /translation="GNPLPPVSLLTLGCPLRQLYAARFPGLYAWVLARNGAMFGPRAA
                     DVGVQRWMNAFCSGDYVGRWLWSHAGAEAALRHPMVDTLDADPFGRADAYTGFDPMPP
                     VDTVLDAAREAEVCLGLGAHTHYFERDQTTVAWMIDYLVRARPVDDADVPAGVAHAVP
                     TVVAAAGQPAAVVPAQASVVSDGSAAPAAAPDSTTPSPSAP"
     gene            713..1303
                     /locus_tag="VIN75_08630"
     CDS             713..1303
                     /locus_tag="VIN75_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY8974269.1"
                     /translation="MNTARLMSIAALASAAALAGCSTTASVTKPGEGDHPSTAESRHA
                     VDASYDETLERLYASNPGTRELVAKARGVLVFPRVISAGLVIGGAYGEGELREHGRVD
                     GYYRTTTGSVGWQIGAQSRALVFLFMTEDALERFKAGHGWSGGVDASVALLTVGANGQ
                     IDANAARAPTLAFVMTNAGIMAGATLEGTKVTRIDY"
     gene            1398..>2052
                     /locus_tag="VIN75_08635"
     CDS             1398..>2052
                     /locus_tag="VIN75_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8974270.1"
                     /translation="MTYAAETHRYDGRMPYRTCGRSGLKLPAISLGLWHNFGDTTSQA
                     TQRQMLRTAFDLGITHFDLANNYGPPYGSAEINFGEHLRRDFRPYRDELVISSKAGWD
                     MWPGPYGQGGGSRKYVLASLDQSLKRLGLDYVDIFYSHRFDPDTPLEETMGALASAVQ
                     QGKALYVGISSYSAGKTREAAAILQRMGVPLLIHQPSYSMLNRWIEDDLLDALDDTGA
                     "
BASE COUNT          300 a          802 c          688 g          262 t
ORIGIN      
        1 cggcaacccg ctgccgccgg tcagcctgct gacgctgggc tgcccgctgc gccagctgta
       61 cgcggcgcgc tttcccgggc tctacgcctg ggtgctggcg cgcaacggcg ccatgttcgg
      121 gccgcgcgcg gccgatgtcg gcgtgcagcg ctggatgaac gcgttctgca gcggcgacta
      181 cgtcgggcgc tggctctgga gccacgccgg cgccgaggcc gccctgcggc acccgatggt
      241 cgacaccctg gacgcggacc cgttcggccg cgccgacgcg tacacgggct tcgacccgat
      301 gccgccggtc gacacggtgc tggacgccgc gcgcgaggcc gaggtctgcc tcggactggg
      361 cgcgcacacg cattacttcg aacgcgacca gaccaccgtc gcctggatga tcgactacct
      421 ggtgcgcgcc cgccccgtcg acgatgccga cgtgccggcc ggcgtcgcgc acgcggtccc
      481 caccgtcgtc gcggccgcgg gccagccggc cgccgtggtg ccggcccagg cctcggtggt
      541 gtccgacggc agcgccgccc cggcggcagc gccggattcg acgacgccgt cgccctcggc
      601 gccctgacga ggcgccctgc ccggcacccc gattgccgcg gaatgggcaa aaccgccatg
      661 ccggcggtcc ggcctcggcg cggtccggcg ccgcaacccc caggaaccga ccatgaacac
      721 cgcccgcctg atgtccatcg ccgcgctcgc gtccgccgcc gcgctcgccg gctgctccac
      781 caccgccagc gtcaccaagc ccggcgaagg cgaccacccg agcaccgcgg aatcgcgcca
      841 cgcggtcgac gccagctacg acgagacgct cgagcgcctg tacgcgtcca accccggcac
      901 gcgcgaactc gtcgccaagg cgcgcggcgt gctcgtcttc ccgcgcgtga tctccgccgg
      961 cctggtgatc ggcggcgcat acggcgaagg ggaactgcgc gagcacggcc gcgtcgacgg
     1021 ctactaccgc accaccaccg gctcggtcgg ctggcagatc ggcgcccagt cgcgcgcgct
     1081 cgtgttcctg ttcatgaccg aggacgcgct ggagcgcttc aaggccggcc acggctggtc
     1141 gggcggcgtc gatgcctccg tcgcgctgct gaccgtcggc gccaacggcc agatcgacgc
     1201 caacgccgcg cgcgccccga cgctcgcgtt cgtgatgacc aacgccggca tcatggcggg
     1261 cgcgacgctc gagggaacga aggtcacgcg catcgactat tgatgtccat tcccgcatcg
     1321 caggcgctcg tctcccgagc cctgcgatcg ccggcggcgg gacagaatcg cggcaaacgc
     1381 cactgcccga gctcgccatg acctacgccg ccgaaaccca ccgctacgac ggccgcatgc
     1441 cctaccggac ctgcggccgc agcggcctga agctccccgc gatctcgctg ggcctgtggc
     1501 acaacttcgg cgacaccacg tcgcaggcca cgcagcgcca gatgctgcgc acggcgttcg
     1561 acctgggcat cacgcacttc gacctcgcca acaactacgg gccgccgtac ggcagcgcgg
     1621 agatcaactt cggcgagcac ctgcggcgcg acttccggcc gtaccgcgac gagctggtga
     1681 tctccagcaa ggccggctgg gacatgtggc ccggcccgta cggccagggt ggcggctcgc
     1741 gcaagtacgt gctcgcgagc ctcgaccaga gcctcaagcg cctgggactg gactacgtcg
     1801 acatcttcta ctcgcaccgc ttcgaccccg acacgccgct ggaggagacc atgggcgcgc
     1861 tcgccagcgc ggtgcagcag ggcaaggcgc tgtacgtcgg catcagcagc tattccgccg
     1921 gcaagacgcg cgaggcggcg gccatcctcc agcgcatggg cgtgccgctg ctgatccacc
     1981 agccgtcgta cagcatgctc aaccgctgga tcgaggacga cctgctcgac gcgctggacg
     2041 acaccggcgc cg
//
LOCUS       DATCRO010000320         6231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1038446, whole genome shotgun sequence.
ACCESSION   DATCRO010000320 DATCRO010000000
VERSION     DATCRO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038446"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VIN75_08640"
     CDS             complement(<1..543)
                     /locus_tag="VIN75_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY8974271.1"
                     /translation="MNQLDQLKAFTVVVADTGNFKQMAQYAPRDATTNPSLILKAVQQ
                     ADYAPLLADTVAAHQGKPLADVVDQVLVRFGLEILKVVPGRVSTEVDARLSFDTAATV
                     ARARRIVGLYEAAGIGRERVLIKIASTWEGIAAARQLEAEGIHCNLTLLFSFCQAVAC
                     GDGGITLVSPFVGRIYDWYKK"
     gene            699..2156
                     /gene="zwf"
                     /locus_tag="VIN75_08645"
     CDS             699..2156
                     /gene="zwf"
                     /locus_tag="VIN75_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8974272.1"
                     /translation="MSFDLVFFGGTGDLTWRKLMPALFQAFRHGKLPEGGRILAVARD
                     ERTDDEYRAFIKERFVEVERSKQPSDEEFARFAELLHYRRMDLSKPDDYAGLKEWIDA
                     RGADTAVLYLATSPHLFPQICEQLGAAGLNGPHVRVVLEKPLGHDLASAQEINRVVKS
                     VFAEKQAFRIDHYLGKPSVQNLMALRFGNALFEPLWNRESIANIQITLAESLGVGTRG
                     DFYDRTGTLRDMVQNHALQLLTMIAMEPPATNDADAIRDEKLKVLRSLKPFNAESVSR
                     DVVRGQYRAGNIDGRAVPGYLEEVKVPAGSHTETFLALRTEVQNWRWAGVPFYIRTGK
                     RLAARDAQIVINFRPVPHPIFGGPNRANQLIIKLQPEDGLELRLQAAKGGLGTPGGVE
                     QLSTVSLDLDFDKAFPTDRVGAYERLLLDAIAGRLNLFVRSDEQEQAWRWVGPILEAW
                     SNDTDGPRSYAAGSWGPAAASSLIARDGFAWAEEE"
     gene            2174..3040
                     /locus_tag="VIN75_08650"
     CDS             2174..3040
                     /locus_tag="VIN75_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8974273.1"
                     /translation="MLERVRASIPALPPAEQRVAKLVLSDPRGFASTPVGELAERAHV
                     SKPTVVRFCRSVGYDGLADFKLKLAGTVNEGVPFVHRSVDEDDGPSELIVKVIDNAVS
                     AMLKYRNNAASHAFERAIAALTAAGTEHRRIDFYGVGNSGIVAQDAQHKFFRLGVNTS
                     ASSDGHVQVMSATMLGPGDCAVIISNSGRSRDLIDVADIARKKGATLIVITASASPLA
                     QMTQAHSQILLAADHPEDFDRYSPMVSRMLHLMIIDILTTGVALRLGQGLRPALQEIK
                     RNLRARRYATPE"
     gene            complement(3289..3633)
                     /locus_tag="VIN75_08655"
     CDS             complement(3289..3633)
                     /locus_tag="VIN75_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8974274.1"
                     /translation="MTALPASLTLKDAQAVLESLRQSLAADGGDTWRIDAAAVTQLDT
                     SALAVLLECARIAAAARRKFEIVNAPARMSDLAHLYGVDGLLGVAAADFRPAANVGES
                     RPQDIRTVPILR"
     gene            complement(3651..4271)
                     /locus_tag="VIN75_08660"
     CDS             complement(3651..4271)
                     /locus_tag="VIN75_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974275.1"
                     /translation="MFQRTISSLIFAAATLGATAAFAQTAPDAMIKQVAGDVIESVKN
                     DKAIQAGDVGLIQGLVDTQVMPHVDFQRMTAATVGRAWKTATPEQQAKLQAEFKTLLI
                     RTYAGALTKVNAQTSVELKPSRNAAEDTDVTVRTAIKGTGGDPIEIDYKLEKQPAEWK
                     IFDVNVLGVWLVDQYKSSFQQQIASGGVDGLINALVAKNKAGTAAK"
     gene            complement(4399..5472)
                     /locus_tag="VIN75_08665"
     CDS             complement(4399..5472)
                     /locus_tag="VIN75_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY8974276.1"
                     /translation="MTSLAPVRAFLRVVVAALALAAVAGCATAPVPGVVDRHTAGPKD
                     PYEPFNRKMFAFNDTLDTYALKPVATAYTKVVPSPIRTGVHNFFGNFSDAWSAVNQLL
                     QGKPADAGSMTLRVLTNTTIGIGGIFDPATSLGLERKSEDLGQTLGVWGLEPGPYLVL
                     PLFGSSDVRDALALPADTYVSPSLLVAPKYWKEVAIDAVGVVDTRAGLLGASQMLDEL
                     AFDRYTFMRDAYITRRRSLVYDGNPPELPDEDSDQDDSKSAPAPAGNGSQSGTKPALP
                     AAPAASGAASGAQSSMVDGDVRVAATAKPSTPPSQPVPTPPPAQPASGVQPAPEPASS
                     PTLPVPRVGPVPVPPPPASGAHG"
     gene            complement(5469..5996)
                     /gene="mlaD"
                     /locus_tag="VIN75_08670"
     CDS             complement(5469..5996)
                     /gene="mlaD"
                     /locus_tag="VIN75_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY8974277.1"
                     /translation="MAKKSTETLVGVFVLLGILALAFLALKAANLTSFNPAKTYTVRA
                     RFDNIGGLKKNAPVRSAGVSVGSVTSITLDKTTYQGLVVMKINEGVQFPVDSSAKILT
                     AGLLGDQYIGIEPGSDDKNLKNGDLIKQTQSAVVLENLIGQLIFNKAADAGSSTAPAP
                     GASLAPAGAASESKK"
     gene            complement(6029..>6231)
                     /locus_tag="VIN75_08675"
     CDS             complement(6029..>6231)
                     /locus_tag="VIN75_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974278.1"
                     /translation="TRDVGNGIVKSAVFGVICTGVALYQGYETQATPEGVAYATTRTV
                     VFSSLAVLAMDFILTAMMFSTS"
BASE COUNT          990 a         2108 c         2178 g          955 t
ORIGIN      
        1 cttcttgtac cagtcgtaga tgcggccgac gaagggcgag accagcgtga tgccgccgtc
       61 gccgcaggcc acggcctggc agaagctgaa cagcagcgtc aggttgcagt ggatgccctc
      121 ggcctccagc tggcgcgcgg cggcgatgcc ctcccacgtc gacgcgatct tgatcaggac
      181 gcgctcgcgg ccgatgccgg cggcctcgta gagcccgacg atgcggcgcg cgcgggcgac
      241 ggtggcggcg gtgtcgaacg acagccgcgc gtcgacctcg gtgctgacgc ggcccggcac
      301 gaccttcagg atctcgaggc cgaagcgcac cagcacctgg tcgaccacgt cggccagcgg
      361 tttgccctgg tgggcggcga cggtgtcggc cagcaagggg gcgtagtcgg cctgctgcac
      421 ggccttcagg atcagcgagg ggttagtggt ggcgtcgcgc ggcgcgtact gcgccatctg
      481 cttgaagttg ccggtatcgg ccaccacgac ggtgaaggcc ttgagttggt cgagctggtt
      541 catgtcatcc cctggaggaa aggcgggtgg cggatttgac cgcgggaaga aacttgtgca
      601 tcattattcc tgaaacaaag ttacaccacc actacttcca ggcaagcccc ggcagcggcg
      661 tcgctccggg ttcccatcct gctccacaag aaaacaccat gtcattcgat ctcgtattct
      721 tcggcggcac cggcgacctc acgtggagaa agctgatgcc cgcgctgttc caggcattcc
      781 gccacggcaa gctgcccgag ggcggccgca tcctggccgt cgcacgcgac gagcgcaccg
      841 acgacgagta ccgcgcgttc atcaaggagc gcttcgtcga ggtcgagcgc tccaagcagc
      901 ccagcgacga ggagttcgcc aggttcgccg agctgctgca ctaccgccgc atggacctgt
      961 ccaagccgga cgactacgcc ggcctgaagg aatggatcga cgcccgcggc gccgacaccg
     1021 cggtgctgta cctggccacc agcccgcacc tgttcccgca gatctgcgag cagctgggcg
     1081 ccgccgggct caacggcccg cacgtgcgcg tggtgctgga aaagccgctg ggccacgacc
     1141 tggccagcgc gcaggagatc aaccgcgtcg tgaagtcggt gttcgccgag aagcaggcct
     1201 tccgcatcga ccactacctg ggcaagccgt cggtgcagaa cctgatggcg ctgcgcttcg
     1261 gcaacgcgct gttcgagccg ctgtggaacc gcgagagcat cgccaacatc cagatcacgc
     1321 tggccgaatc gctgggcgtg ggcacgcgcg gcgacttcta cgaccgcacc ggcacgctgc
     1381 gcgacatggt gcagaaccac gcgcttcagc tgctgacgat gatcgcgatg gagccgccgg
     1441 ccaccaacga cgccgacgcc atccgcgacg agaagttgaa ggtgctgcgc tcgctcaagc
     1501 cgttcaacgc cgagagcgtg tcgcgcgacg tcgtgcgcgg ccagtaccgc gccggcaaca
     1561 tcgatggccg tgccgtcccg ggctacctcg aggaagtgaa ggtgcccgcg ggcagccaca
     1621 ccgagacgtt cctcgcgctg cgcaccgagg tgcagaactg gcgctgggcc ggcgtgccgt
     1681 tctacatccg cacgggcaag cgcctggcgg cgcgcgacgc gcagatcgtc atcaacttcc
     1741 gccccgtgcc gcacccgatc ttcggcggcc cgaaccgcgc caaccagctg atcatcaagc
     1801 tgcagcccga ggacggcctc gagctgcgcc tgcaggcggc caagggcggc ctgggcacgc
     1861 cgggcggcgt ggagcagctc agcacggtct cgctggacct cgacttcgac aaggccttcc
     1921 cgaccgaccg cgtcggcgcc tacgagcgcc tgctgctgga cgccatcgcc ggccgcctga
     1981 acctgttcgt gcgcagcgac gagcaggagc aggcctggcg ctgggtcggc ccgatcctgg
     2041 aggcctggtc caacgacacc gacggcccgc gctcgtacgc ggccggcagc tggggccccg
     2101 cggcggcgag ttcgctgatc gcgcgcgacg gcttcgcgtg ggccgaggaa gagtgacggc
     2161 ataacccatg gccatgctcg aacgcgtccg cgcgtccatc cccgccctgc cgccggccga
     2221 acagcgcgtc gccaagctcg tgctgagcga cccgcgcggg ttcgcgtcca cgccagtggg
     2281 cgagctggcg gagcgcgcgc acgtgtccaa gccgaccgtc gtgcgcttct gccgcagcgt
     2341 gggctacgac ggcctggccg acttcaagct gaagctggcc ggcaccgtca acgagggcgt
     2401 gccgttcgtg caccgctcgg tggacgagga cgacggcccg agcgagctga tcgtcaaggt
     2461 gatcgacaac gcggtcagcg cgatgctcaa gtaccgcaac aacgcggcca gccacgcgtt
     2521 cgagcgcgcg atcgcggcgc tgaccgccgc cggcaccgag cacaggcgca tcgacttcta
     2581 cggcgtcggc aactccggca tcgtcgcgca ggacgcgcag cacaagttct tccgcctcgg
     2641 cgtcaacacc tcggccagca gcgacggcca cgtgcaggtg atgagcgcca cgatgctcgg
     2701 gccgggcgac tgcgcggtca tcatcagcaa ctcgggccgc agccgcgacc tgatcgacgt
     2761 ggccgacatc gcgcgcaaga agggcgcgac actgatcgtc atcacggcca gcgcctcgcc
     2821 gctcgcgcag atgacgcagg cgcactcgca gatcctgctg gccgccgacc atcccgagga
     2881 cttcgaccgc tacagcccga tggtctcgcg catgctgcac ctgatgatca tcgacatcct
     2941 gaccacgggc gtcgcattgc gcctcggcca gggcctgcgg ccggcgctgc aggagatcaa
     3001 gcgcaacctg cgcgcgcgcc ggtacgcgac gccggagtga cctgaagggg tctggcctct
     3061 ccgccgtccg cgacaccctt tcgatcgaag aaccggtctc gaatcgcgga cggaggcgaa
     3121 ggcctgaccc cgatcatctg acgacgaggg ctgacgacgg ggtcaggtct tgaatcgtgc
     3181 actttcgcca ctgcacgatt caagacctga ccccgcaaga ttcaagacct gaccccggaa
     3241 tgagaaagac ccgccgcggc gggtcttcct gcttggggcg gcgggcactc agcgcaggat
     3301 cggcaccgtc cgaatgtcct gcggcctgga ctcgcccacg ttggccgcgg gcctgaagtc
     3361 ggccgccgcg acgcccagca ggccgtcgac gccgtacagg tgcgcgagat cgctcatgcg
     3421 cgcgggggca ttgacgatct cgaacttgcg cctggccgcc gccgcgatgc gcgcgcactc
     3481 gagcagaacc gccagcgccg acgtgtcgag ctgcgtcacg gcggccgcgt cgatgcgcca
     3541 ggtgtcgccg ccgtccgcgg cgagcgactg gcgcagcgac tccagcaccg cctgcgcgtc
     3601 cttgagcgtc aggctcgccg gcagcgcggt cattgcaccc gattgcgtca ttacttggcc
     3661 gccgtgccgg ccttgttctt cgcgacgagc gcgttgatca ggccgtcgac gccacccgag
     3721 gcgatctgct gctggaagct gctcttgtac tggtcgacca gccagacgcc cagcacgttg
     3781 acgtcgaaga tcttccactc ggccggctgc ttctcgagct tgtagtcgat ctcgatcggg
     3841 tcgccgcccg tgcccttgat ggcggtgcgc acggtgacgt cggtgtcctc ggcggcgttg
     3901 cggctgggct tgagttcgac cgacgtctgc gcgttgacct tggtcagcgc gccggcgtac
     3961 gtgcggatca gcagcgtctt gaactcggcc tgcagcttgg cctgctgctc gggcgtggcc
     4021 gtcttccagg cgcggccgac ggtggcggcg gtcatgcgct ggaagtcgac gtggggcatg
     4081 acctgcgtgt cgacgaggcc ctggatgagg ccgacgtcgc cggcctggat cgccttgtcg
     4141 ttcttgaccg actcgatgac gtcaccggcc acctgcttga tcatggcgtc cggcgcggtc
     4201 tgcgcgaacg cggcggtggc gccgagggtg gcggcggcga agatcagcga agaaatcgtg
     4261 cgttgaaaca ttcctgtcct ttcgagcggg agatcgctcc attccttaac gtgggccgcc
     4321 cggaagcggt tcacagggcc gacatgcaaa gaaacgtaca gctcgacggc gggccgattg
     4381 tcgcctgtcg cgccgggctc agccgtgcgc gccggatgcc gggggcggcg ggacgggcac
     4441 cgggccgaca cgcggcaccg gaagcgtggg cgacgaggcc ggctcgggcg cgggctgcac
     4501 gccgctggcc ggctgagccg gcggcggggt gggcaccggc tgcgacggcg gcgtggacgg
     4561 cttggcggtg gccgcgacac ggacgtcgcc gtccaccatc gacgactgcg cgccggaggc
     4621 cgcgcccgac gccgccggag ccgcggggag cgccggcttg gtgccgctct ggctgccgtt
     4681 gcctgccgga gcgggcgccg acttcgagtc gtcctggtcg gaatcctcgt ccggcagttc
     4741 gggcgggttg ccgtcgtaga ccaggctgcg gcggcgcgtg atgtaggcgt cgcgcatgaa
     4801 ggtgtagcgg tcgaacgcca gttcgtcgag catctggctc gcgcccagca ggcccgcgcg
     4861 ggtgtcgacc acgccgaccg cgtcgatggc cacttccttc cagtacttgg gcgccaccag
     4921 cagcgacggc gacacgtagg tatcggccgg cagcgcgagg gcatcgcgga cgtcggacga
     4981 gccgaacagc ggcagcacca ggtacgggcc gggctcgagg ccccagacac ccagggtctg
     5041 tccgagatcc tcggacttgc gctccaggcc caggctcgtg gccggatcga agatgccgcc
     5101 gatgccgatg gtggtgttgg tgagcacgcg cagcgtcatc gagccggcgt cggcgggctt
     5161 gccctgaagc agctggttga cggccgacca cgcgtccgag aagttgccga agaagttgtg
     5221 cacgccggtg cggatcggcg acggcacgac cttggtgtag gccgtggcga ccggcttgag
     5281 cgcataggtg tccagcgtgt cgttgaacgc gaacatcttg cggttgaacg gctcgtacgg
     5341 atccttcggg ccggcggtgt ggcgatcgac gacccctggc acgggcgccg tggcacagcc
     5401 ggcgacggcg gccagggcga gcgcggcgac gacgacgcgc aggaaggcgc gaaccggggc
     5461 gagggaggtc atttcttgga ttccgaagcg gccccggcag gggcaagcga cgcgccaggg
     5521 gcgggcgcag tggagctgcc ggcgtcggcg gccttgttga agatcagctg gccgatcagg
     5581 ttctcgagca cgacggcgga ctgcgtctgc ttgatgaggt cgccgttctt caggttcttg
     5641 tcgtccgaac cgggctcgat gccgatgtac tggtcgccca gcaggccggc ggtcaggatc
     5701 ttggcggacg agtcgaccgg gaactgcacg ccctcgttga tcttcatgac caccaggccc
     5761 tggtaggtgg tcttgtccag ggtgatcgac gtcaccgagc cgaccgacac gccggcgctg
     5821 cgcacgggcg cgttcttctt gagcccgccg atgttgtcga agcgggcgcg gacggtgtac
     5881 gtcttggccg ggttgaagct ggtcaggttg gccgccttca gcgccaggaa tgccagcgcg
     5941 aggatgccca gcaagacgaa aacgcccacc agggtctcag tgcttttctt tgccatgtcg
     6001 gattccgatt cctacccttg acgccgcttt aggacgtcga aaacatcatt gccgtgagga
     6061 tgaagtccat cgccagcacc gccagcgaag agaacaccac cgtgcgcgtg gtcgcatagg
     6121 ccacgccctc gggcgtcgcc tgcgtctcat agccctggta cagggcgact ccggtgcaga
     6181 tcacgccgaa cacggcggac ttgacgatgc cgttgcccac gtcgcgcgtg a
//
LOCUS       DATCRO010000321         7113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_566268, whole genome shotgun sequence.
ACCESSION   DATCRO010000321 DATCRO010000000
VERSION     DATCRO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566268"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VIN75_08680"
     CDS             <1..1059
                     /locus_tag="VIN75_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY8974279.1"
                     /translation="EQDLPIGWVAGVTLACLVPIGLLLADQSNGAGLGSATAILVVGG
                     IAFIALMSFFVAAVCGYMAGLIGSSNSPLSGVGIIVVISAALLVAIGVKPMLPAGAGP
                     ALVAFTLFVTAVIIAAGTVANDNLQDLKTGQLVDATPWKQQVALAIGTVAGALVIPPV
                     LDLLQKAYGFAGAPGATAKALAAPQAALISALAKGVLGGDLDWNLLGKGVLLGVVLVV
                     VDEIIKRTRKPGSHAQLPPLAVGLGIYLPMATTLMVVVGAFVGHAWERHADTTKRPVS
                     ARQLGVLLASGMIVGEGLFGVAIAALTVFSGKDAPLAVVGDGFAGTALWLGGIGFVVA
                     TALLYRWLSRLSAKVAAA"
     gene            1073..1462
                     /locus_tag="VIN75_08685"
     CDS             1073..1462
                     /locus_tag="VIN75_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974280.1"
                     /translation="MLDATPDSVRLFAEHLLELMAIRCGGARLYLAAAAVGADGGLNH
                     YAVNQRGDVLVRGDVALSASRVLDEAPELRVTTLASLPFAQLGAIWIALCDGAPGQGL
                     FTLPELPPRWAPYTEVVRDVLGGPEAR"
     gene            complement(1824..2354)
                     /gene="pal"
                     /locus_tag="VIN75_08690"
     CDS             complement(1824..2354)
                     /gene="pal"
                     /locus_tag="VIN75_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY8974281.1"
                     /translation="MRISPLSITLTLSFLAACSTPPKATEPAAPPPPAAPVVPAPKPA
                     PAAPAVDDAQALAAKLRQQVADLDSKSVFFDFDSFVVKSEYDATLHAQADFLKAHPGS
                     RLTVQGNTDERGGSEYNLALGTKRAEAVKKQLGLLGVSTDRIEATSFGKEKPRATCHD
                     ESCWSQNRRDDFVNKP"
     gene            complement(2450..3796)
                     /locus_tag="VIN75_08695"
     CDS             complement(2450..3796)
                     /locus_tag="VIN75_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974282.1"
                     /translation="MRAEATHSLRTRLLGWLMAAIVLAAIAQAAFAYFTALSQADVIF
                     DRHLRTMATALISGTSLGSLPPSGEVNADRAGEDFVVQLWNAKGEPIFRTAAHQKLRQ
                     PAQPGFSTVQALDGRDYRVYATTAPGLTVLVAQDMTARRDMARTLAVRTALPELPTAP
                     LLALLVWWVVSRSLAPVARLRAQMAARKVDELAPLADRGLPAEIQPLIQELNLLFERL
                     RQAFDSQRVFVADAAHELRSPLAALKIQLQGLQRAGDDPSRELAVKRLAAGIDRAGHL
                     VDQMLVLARQEASVAEGVALEPLDLAQPGFLALSDTLASAQARQIDLGVHRAESVTVA
                     GNAESLRILVRNLLDNGVKYTPAGGTVDLSVHATPEGALVTVEDSGPGIPEKDRARAL
                     DRFYRVVNGAGATGSGLGLAIVKSIADMHGATITLDDSQRLGGLRVRVSFGLRSAA"
     gene            complement(3793..4452)
                     /locus_tag="VIN75_08700"
     CDS             complement(3793..4452)
                     /locus_tag="VIN75_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974283.1"
                     /translation="MRLLFVEDDHMIGETVVDVLRAENYAVDWIKDGQTAQTVLRTQA
                     YDLLLLDLALPGKDGIEVLRALRAARLRIPVLIATARDSVEQRIAGLDAGADDYILKP
                     YDLGELLARVRALLRRAAGRAEPVYEHLGVTLNPATREATVNGQPVLLSSREWAVLEP
                     LIARPGAVLSRAQLEEKIFGFDDSIASNAVEVYVHGLRKKLGANLIQNVRGLGYLVPR
                     E"
     gene            complement(4555..5394)
                     /locus_tag="VIN75_08705"
     CDS             complement(4555..5394)
                     /locus_tag="VIN75_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974284.1"
                     /translation="MSALNEHDRIAVMAYVDGEMPPEEVALFEPRLALEPALADAVAR
                     ERALRSSLQSAYAPVLDEPMPAGLLDLLAIPDAAAKAATIHLVAAPAPAAAANQALPA
                     GANDAARAALPHARRWAWPQFGAMAASLVLGVLFGARLFSPHPAPGGDTLALAVAPDG
                     AITAQGALRHALEDDIAGTVLDPNAKVGVGLTFRDHARQYCRTFTLDSESSGIACKQA
                     DGWVVAHLEHGRNAREGEQGAYRTAGSPLSPALLQAIDAMRDGDTLDAAGEAAAKAKG
                     WKP"
     gene            complement(5515..6018)
                     /locus_tag="VIN75_08710"
     CDS             complement(5515..6018)
                     /locus_tag="VIN75_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8974285.1"
                     /translation="MSQSPLALRAEIAALLPRLRRFARSVTHHREDADDLVQIAVERA
                     LKHLDHWQDGTRLDSWLFRIMKNAWIDEVRARGRRDEVLAPEEAGEHVGYSETGSRDI
                     RLSIEKAMARLPEDQRMAVSLVLVEGLPYKEASEVLDIPIGTLTSRLARGRDALQAML
                     GDGDLTP"
     gene            6080..>7113
                     /locus_tag="VIN75_08715"
     CDS             6080..>7113
                     /locus_tag="VIN75_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8974286.1"
                     /translation="MSYTTCRFLALAAALLSGAAHAQLRLPSLGSTLSPLTRSPLVQR
                     VDPLEGDVLAPVDLAAARLDETAARARAHRRELDRDPRGELIVRAELLAQPSGPAARD
                     ALLAVGFEPLREDALDGLDDDALLVLRAPSGMSLADALARARALDPAGAYDFHHVYLG
                     SGSVGAPSSTASSTPAPAVQAAVASDAPLQVGLIDSGVDASHPAFRHATIRRHGCSDG
                     DHPAPHGTAVASLMVGEAAHFQSALPRAVLVAGDVYCDAPTGGSVEAIARELAWMARQ
                     RVAVVNISLVGPPNQILQRLVERAIGQGVLIVAAVGNDGPAAPPLYPASWPGVVGVGA
                     VDGKLRLLVEG"
BASE COUNT          890 a         2517 c         2662 g         1044 t
ORIGIN      
        1 gagcaggacc tgccgatcgg ctgggtcgcg ggcgtcacgc tcgcctgcct ggtgcccatc
       61 ggcttgctgc tggccgacca gagcaacggc gccggcctgg gttcggccac cgccatcctg
      121 gtcgtcggcg gcatcgcctt catcgcgctg atgagcttct tcgtggccgc tgtgtgcggc
      181 tacatggccg gcctgatcgg ctcgagcaac agcccgctgt cgggcgtggg catcatcgtc
      241 gtgatctcgg cggcgctgct ggtggcgatc ggggtcaagc ccatgctgcc ggccggcgcg
      301 ggccccgcac tggtggcgtt cacgctgttc gtcaccgccg tcatcatcgc ggcgggcacg
      361 gtcgccaacg acaacctgca ggacctgaag acgggccagc tggtcgacgc cacgccgtgg
      421 aagcagcagg tggcgctggc gatcggcacg gtggccgggg ccctcgtcat cccgccggtg
      481 ctggacctgc tgcagaaggc ctacggcttc gccggcgcgc ccggcgccac cgccaaggcg
      541 ctcgccgcgc cgcaggccgc gctgatctcg gcgctcgcca agggcgtgct gggcggcgac
      601 ctcgactgga acctgctggg caagggcgtg ctgctgggcg tcgtgctcgt cgtcgtcgac
      661 gagatcatca agcgcacgcg caagcccggc agccacgcgc agctgccgcc gctggccgtg
      721 ggcctgggca tctacctgcc gatggccacg acactgatgg tcgtggtcgg cgccttcgtc
      781 ggccacgcgt gggagcgcca cgccgacacc acgaagcgcc cggtgtcggc caggcagctg
      841 ggcgtgcttc tggccagcgg catgatcgtc ggcgagggcc tgttcggcgt ggccatcgcg
      901 gcgctgaccg tgttcagcgg caaggacgcg ccgctcgcgg tcgtgggcga cggtttcgcc
      961 ggcaccgcgc tgtggctcgg cggcatcggc ttcgtcgtcg cgacggcgct gctgtatcgc
     1021 tggctcagcc gcctgagcgc caaggtggcg gccgcgtagg cggcgcggcg cgatgctgga
     1081 cgccacgccc gacagtgtcc ggctcttcgc cgagcacctg ctcgagctga tggcgatccg
     1141 ctgcggcggc gcgcggctgt acctggccgc cgcggcggtg ggcgcggacg gcgggctgaa
     1201 ccactacgcc gtgaaccagc gcggcgacgt gctggtgcgc ggcgatgtcg ccttgtccgc
     1261 gagccgcgtg ctcgacgaag caccggagct gcgcgtgacc acgctcgcga gcctgccgtt
     1321 cgcgcagctg ggcgcgatct ggatcgcgct gtgcgacggc gcgccggggc agggcctgtt
     1381 caccctgccg gagctgccgc cgcggtgggc gccctacacc gaggtcgtgc gcgatgtgct
     1441 gggcgggccg gaagcgagat gacacctgtt tcgcgcgagg tgccaaaggg tgccgcgcgg
     1501 cagcgccaag ccgtcatcct gcgcgcagcg aagcggagtc gcaggatcca tccccaggga
     1561 aatcgcctcg gtccgcgtgg atcctgcggc tgcgcgcagg atgacagctc gtgatcccga
     1621 cgccgcgcga ggccggggcc ggagcacgcc accctgctgc atgggcggcg gtcggcatgc
     1681 gtccaggcgc atggctgtgc gcccgcgcgg cgcgtgcgcg ccccaatgga agaaggccct
     1741 cgcgggcctt cttggttgca tgcggacccg tggcccgcgc gcccggccat cccaacgacg
     1801 gcctcgttgt cgaacttgaa gcctcagggc ttgttgacga aatcgtcgcg gcggttctgc
     1861 gaccagcacg attcgtcgtg gcaggtcgcg cgcggcttct ccttgccgaa gctcgtcgcc
     1921 tcgatgcggt cggtggacac gcccagcagg ccgagctgct tcttcacggc ttcggcgcgc
     1981 ttggtgccca gggccaggtt gtactcggag ccgccgcgtt cgtcggtgtt gccctgcacg
     2041 gtcagcctgg agccggggtg ggccttgagg aagtcggcct gcgcatgcag cgtggcgtcg
     2101 tactcggact tgacgacgaa cgagtcgaag tcgaagaaca cgctcttgga gtcgaggtcg
     2161 gcgacctgct ggcgcagctt ggcggcgagc gcctgggcgt cgtcgacggc gggcgccgcg
     2221 ggggcgggct tgggggccgg gacgaccggc gcggccggcg gcggcggagc ggccggctcg
     2281 gtggccttgg gcggggtgct gcaggctgcc aggaaggaca gggtcagggt gatgctcagg
     2341 ggagagatgc gcacggggat cctcgtggtg gtacgggagg tcgctcgaaa ccgattcagg
     2401 gggcggccga gttgccgggg aattctgggc gtgctgtctt aggcggcgct taagcggctg
     2461 aacgcaagcc gaacgacacg cgcacgcgca ggccgccgag ccgttgcgag tcgtccaggg
     2521 tgatcgtggc gccgtgcatg tccgcgatcg acttgacgat cgccaggccc agcccgctgc
     2581 ccgtcgcgcc cgcgccgttg acgacgcggt agaagcgatc gagcgcgcgc gcccggtcct
     2641 tctccgggat gcccgggccg ctgtcctcca ccgtcaccag cgcgccctcg ggcgtcgcat
     2701 gcacgctgag gtcgacggtg ccgccggcgg gcgtgtactt cacgccgttg tccagcaggt
     2761 tgcgcaccag gatgcgcagc gactccgcgt tgcccgccac ggtgacgctc tcggcgcggt
     2821 gcacgcccag gtcgatctgc cgcgcctgcg cgctggccag cgtgtcgctg agcgcgagga
     2881 agccgggctg cgccagatcg agcggctcca gcgccacgcc ctcggccacg ctggcctcct
     2941 ggcgcgcgag caccagcatc tggtcgacga gatggccggc gcggtcgatg cccgcggcca
     3001 gccgcttgac ggcgagctcg cggctcgggt cgtcgccggc gcgctgcagg ccctgcagct
     3061 ggatcttgag cgcggccagc ggcgagcgca gctcgtgcgc ggcatcggcc acgaacacgc
     3121 gctgcgagtc gaaggcctgg cgcaggcgct cgaacagcag gttcagctcc tggatcagcg
     3181 gctggatctc ggcgggcagg ccgcgatcgg ccagcggcgc gagctcgtcg accttgcggg
     3241 cggccatctg cgcgcgcagc cgcgccacgg gcgccagcga gcggctgacc acccaccaca
     3301 ccagcagggc cagcagcggc gcggtcggca actcgggcag ggcggtgcgc accgccagcg
     3361 tgcgggccat gtcgcggcgc gcggtcatgt cctgcgccac cagcaccgtc agcccgggcg
     3421 cggtcgtcgc atagacgcga tagtcgcggc cgtcgagcgc ctgcacggtc gagaagccgg
     3481 gctgcgccgg ctggcgcagc ttctggtgcg ccgcggtgcg gaagatcggc tcgcccttgg
     3541 cgttccacaa ctgcacgacg aagtcctcgc ccgcgcgatc ggcgttgacc tcgccgctgg
     3601 gcggcagcga gccgagcgac gtgcccgaga tcagcgccgt ggccatcgtg cgcaggtggc
     3661 ggtcgaagat gacgtcggcc tgcgacagcg cggtgaagta cgcgaacgcg gcctgcgcga
     3721 tggccgccag cacgatggct gccatcagcc atccgagcag gcgcgtgcgc agcgagtggg
     3781 tcgcctccgc cctcattcgc gcggcaccag gtagcccagt ccgcgcacgt tctggatcag
     3841 gttggcgccc agcttcttgc gcaggccgtg cacgtagacc tcgacggcgt tgctcgcgat
     3901 ggagtcgtcg aagccgaaga tcttctcctc gagctgcgcg cgcgacagca ccgcgccggg
     3961 acgcgcgatc agcggctcga gcaccgccca ctcgcgcgag gacagcagca ccggctgccc
     4021 gttgaccgtg gcctcgcgcg tggccgggtt cagcgtgacg cccaggtgct cgtagacggg
     4081 ctcggcccgt ccggcggcgc gccgcagcag ggcgcgcacg cgcgccagca gctcgcccag
     4141 gtcgtagggc ttgaggatgt agtcgtcggc gcccgcgtcc agtccggcga tgcgctgctc
     4201 caccgaatcg cgcgccgtgg cgatcagcac cgggatgcgc agcctggcgg cgcgcagcgc
     4261 acgcagcacc tcgatgccgt ccttgccggg cagcgccaga tcgagcagca gcaggtcgta
     4321 ggcctgcgtg cgcagcaccg tctgcgccgt ctggccgtcc ttgatccagt cgaccgcgta
     4381 gttctcggcg cgcaggacgt ccacgacggt ctcgccgatc atgtggtcgt cctcgacgaa
     4441 aagcagtctc atgcgtgcga ttgtgtgccc gggccaacgg cggaatctgc gggcggtcgc
     4501 ttagggcacg cttaagtcct gccgatgtgc tgtgccggag atgcgcgcgc ggtcctacgg
     4561 cttccagccc ttcgccttgg cggccgcctc gcccgcggcg tcgagggtgt cgccatcgcg
     4621 catcgcgtcg atcgcctgca gcagcgccgg actcagcggg ctgccggcgg tgcggtaggc
     4681 gccctgctcg ccctcgcgtg cgttgcgtcc gtgctccagg tgggccacca cccagccgtc
     4741 ggcctgcttg cacgcgatgc ccgaggactc gctgtccagc gtgaaggtgc ggcagtactg
     4801 ccgggcatgg tcgcggaagg tcaggccgac gccgaccttg gcgttcgggt cgagcaccgt
     4861 gccggcgatg tcgtcctcga gcgcgtggcg cagcgcgccc tgcgcggtga tcgcgccgtc
     4921 gggcgcgacg gcgagcgcca gcgtgtcgcc gcccggcgcc gggtgcggcg agaacaggcg
     4981 cgcgccgaac agcacgccca gcaccagcga cgcggccatc gcgccgaact gcggccaggc
     5041 ccagcggcgc gcgtgcggca gcgcggcgcg cgcggcgtcg ttggcgccgg cgggcagggc
     5101 ctggttcgca gcggcggccg gcgcgggcgc cgcgaccaga tggatggtcg cggccttggc
     5161 ggcggcgtcg gggatggcca gcaggtcgag caggccggcc ggcatcggct cgtcgagcac
     5221 cggcgcatag gccgactgca ggctcgagcg cagcgcgcgc tcgcgcgcga ccgcgtccgc
     5281 gagcgcgggc tcgagcgcga ggcgcggctc gaacagcgcg acctcctcgg gcggcatctc
     5341 gccgtcgacg taggccatca cggcgatgcg atcgtgttcg ttcaaagcag acattggata
     5401 gcctccatcc tgcggatttc ggcgcgagcg ccttcgggcg gccgtgcgtc gctcatcagg
     5461 caacctccgc gctgcgcgct gcggtcaatt cgcacttcgg gcggccgtgc gggctcatgg
     5521 cgtcaggtct ccgtcgccca gcatggcctg cagcgcgtcg cggccgcgcg cgagccggct
     5581 ggtgagcgtg ccgatgggga tgtccagcac ctcggacgct tccttgtagg gcaggccctc
     5641 gaccagcacc aggctcaccg ccatgcgctg gtcctccggc agccgggcca tcgccttctc
     5701 gatcgacagc cggatgtcgc ggctgcccgt ctcggaatag ccgacgtgtt cgccggcctc
     5761 ctcgggggcg agcacctcgt cgcggcggcc gcgcgcgcgc acttcgtcga tccaggcgtt
     5821 cttcatgatt cgaaacagcc agctgtccag tcgggttcca tcctgccaat ggtcgaggtg
     5881 cttcaacgca cgctcgaccg cgatctgcac gaggtcgtcc gcgtcctccc ggtgatgggt
     5941 gacggatcgg gcgaaacggc gcagccgcgg cagcagggcc gcgatctccg cgcgcaacgc
     6001 aaggggcgat tggctcatga catgatggga gaacgtcgtg gcgatggatt ttcttccatc
     6061 gctccaacgt tattcgaaca tgagctacac aacttgcagg ttccttgcgc tcgcggccgc
     6121 cctgctgtcg ggcgccgccc acgcgcagct gcgcctgccc tcgctgggtt cgaccctgtc
     6181 gccgctgacg cgatcgccgc tggtgcagcg cgtcgatccg ctggagggcg acgtgctggc
     6241 gccggtcgac ctcgccgccg cgcggctgga cgagaccgcc gcgcgcgccc gcgcgcaccg
     6301 gcgcgagctc gaccgcgatc cccgcggcga gctgatcgtg cgcgccgagc tgctggccca
     6361 gccctccggc cccgccgcgc gcgacgcact gctggccgtc ggcttcgagc cgctgcgcga
     6421 ggacgcgctc gacggcctgg acgacgacgc gttgctggtg ctgcgcgcgc cgtcgggcat
     6481 gtcgctggcg gacgcgctgg cgcgggcgcg cgcgctcgac cccgcgggcg cgtacgactt
     6541 ccatcacgtc taccttggca gcggcagcgt gggcgcgccg tcgtcgacgg cgtcgtccac
     6601 gccggccccg gcggtgcagg cggcagttgc gagtgatgcc ccgctgcagg tcggcctgat
     6661 cgacagcggc gtcgacgcgt cgcacccggc cttccgccac gcgacgatca ggcgccacgg
     6721 ctgcagcgac ggcgaccatc cggcgccgca cggcacggcc gtcgcctcgc tgatggtggg
     6781 cgaggccgcg cactttcagt ccgcgctgcc gcgcgcggtg ctggtggccg gcgacgtcta
     6841 ctgcgacgcg cccaccggcg gctcggtgga ggcgatcgca cgcgagctgg cgtggatggc
     6901 caggcagcgc gtggccgtcg tcaacatcag cctggtcggg ccgcccaacc agatcctgca
     6961 gcgcctcgtc gaacgcgcga tcgggcaggg cgtgctgatc gtcgcggcgg tcggcaacga
     7021 cgggcccgcg gcgccgccgc tgtatccggc ctcgtggccc ggcgtggtgg gcgtgggcgc
     7081 ggtcgacggc aagctgcgac tgctggtcga ggg
//
LOCUS       DATCRO010000322         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_431185, whole genome shotgun sequence.
ACCESSION   DATCRO010000322 DATCRO010000000
VERSION     DATCRO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431185"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="urtA"
                     /locus_tag="VIN75_08720"
     CDS             complement(<1..1262)
                     /gene="urtA"
                     /locus_tag="VIN75_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEY8974287.1"
                     /translation="MTVSRRLFNLSLVAAAAAVASSGALAADTIKVGILHSLSGTMAI
                     SETVLKDTALMTIAEINAKGGVLGKQLEPVVVDPASNWPLFAEKARQLIAQDKVAVVF
                     GCWTSVSRKSVLPVFEELNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYLMSK
                     DGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVADADIMEEYTPFGHSDYQSIIAKI
                     KKFSSEGKKTAVVSTINGDSNVPFYKELGNQGVKATEVPVVAFSVGEEELRGVDTKPL
                     VGNLAAWNYFMSVKNPTNTAFIKAWSDYAKKNNLAGDKDKPLTNDPMEATYVGIHMWA
                     QAVEKAKSTDTDKVIAAMAGQTFKAPSGFVLEMDPKNHHLHKPVFIGEVKADGQFNVV
                     WKTPGPVQADPWSDYIPENKGKKNVPEK"
     gene            complement(1387..2343)
                     /locus_tag="VIN75_08725"
     CDS             complement(1387..2343)
                     /locus_tag="VIN75_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974288.1"
                     /translation="MSSSADGWGSAGAGRVLIVDDVPDNLAVLHDALDESGYTVLVAT
                     DGQSAIARAAQAQPDIVLLDALMPGMDGFEVARRMKADQSIAPIPIIFMTALTDTEHV
                     LAAFAAGGVDYVPKPIRPREVVARIAAHLRSARHATQARNALDAFGHASMALRPRDGR
                     VLWRTPLARELMERHFGGALDGAVRAPAGLCAWVHARLAAGDAGTVPPHWSSIAGGRR
                     LQCTLHPASAEDEWLVVLTESDNGTAIEALALRFRLTAREAEVLYWVAQGKTNRDVGE
                     ILGTSPKTVTKHLERVFEKLGVETRTAAASRVLGTLPEVMRR"
     gene            complement(2348..5896)
                     /locus_tag="VIN75_08730"
     CDS             complement(2348..5896)
                     /locus_tag="VIN75_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974289.1"
                     /translation="MTSTDAAPDSLAPAPQRVVKVRRDYNSWVARESLEDYALRYTPQ
                     SFRRWSEARVANTAFGAASFLVLEAVGGTLLVQAGFVNAFWAILATGFVIALAGWPIA
                     LHAARHGLDMDLLTRGAGFGYLGSTITSLVYASFTFIFFALEAAVMAYALELAFGIPP
                     AWGYLACALAVLPLVAHGVTAIGRLQVWTQPLWLALLVLPYLFVFHANPQALAGLWRY
                     AGQDGGGGTFDMLRFGAATTVGIALMTQMGEQADYLRFMPALTAANRRRWFTSVAIGG
                     PGWVLPGVLKMLGGALLAWIAIGHSVPADRAVDPNQMYLAAWDTVFSRPAAAVAATTV
                     FVLLSQLKINVTNAYAGSLAWSNFFSRLTHSHPGRVVWVAFNTAIALMLMELDVFTML
                     GQVLGLYANIAISWLMAVVADLVVNKPLGLSPKGIEFRRAYLYDVNPVGVGAMGIASV
                     LGIAAHLGAFGPLAQAFSALISLVAAFTASPLIAWATKGRYYLARTYEQGDAAERRPD
                     DPPIPVHPAAAAAPAAAQPRMRRCCICEREYEQADIAHCPAYQGAICSLCCSLDARCG
                     DLCKPHARLATQARDAAHALLPRRLWPAFDGGLGAWLALMLPVAALLATVCSLLLRQE
                     LQALGDLPAAAGTLRRSCVGVFAVLLLAGGVAGWWAVLARQSRRVAQEESNRQTQALG
                     EQTALLRLEIDSHRRTDAALQLAKAAADAANQAKSRYISALSHELRTPLNSVIGYAQL
                     LGDDPALPAHRRQAVSVIRRGGEHLLSLIEGTLDLARIEGGKVSLAIAPVSLRQLLDE
                     LADMFALQARAKGLDFAAEFDAGLPRAVRTDERKLRQVLINVLGNAVKFTDRGEVRLR
                     VRHEREIAQFDIEDTGPGIAATDLERVFEPFARGSAASDGKGPGTGLGLTIAKMLTDV
                     MGGEMTVRSTPGSGTAFRIRLFLPAQSAAAASCASPPRRVGYRGDRRRVLAVDNEQVD
                     RELLRQLLVPLGFEVELASSGEDAIARLRQGARPDAILMDLAMPGIDGWETIRRLRAL
                     GLSRAPVAIVSANAFDRRLDNDLGLQADDYVLKPVRADDLLDWLGRALAVDWCTDPAV
                     DPAHETADPQATARQATAPQAEALPVIEALRALSEQVRLGYPRGIHRWLDHVDATEPA
                     CAAFTGRLRAMARQFRLDAMEAVLAEALREREAGLG"
     gene            5968..6270
                     /locus_tag="VIN75_08735"
     CDS             5968..6270
                     /locus_tag="VIN75_08735"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY8974290.1"
                     /translation="MNLTPREKDKLLLFTAALLAERRKARGLKLNHPEAIALISAAIL
                     EGARDGRSVAELMSEGRRVLTRADVMPGIAEMIPEIQVEATFPDGTKLVTVHQPIP"
     gene            6326..6925
                     /locus_tag="VIN75_08740"
     CDS             6326..6925
                     /locus_tag="VIN75_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY8974291.1"
                     /translation="MTLTNSRHPLRIALSLASCALPIAAMAHPGSLAEHAARSHGFID
                     GFAHPFTGLDHLGAMLAVGLWSARSLRRAWIAPAAFVACLLAGALATSNGLAVAGAEP
                     MIAASLLVIGLLLAARAALPAAAAAAVVGGFAFFHGASHGVELGAGPALAGMVLATAL
                     LHLAGIAIGLALRGRSAWWTRIAGAGLAMFGLVLLGAGA"
     gene            6925..7242
                     /locus_tag="VIN75_08745"
     CDS             6925..7242
                     /locus_tag="VIN75_08745"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY8974292.1"
                     /translation="MASPVHPGELIVDGPDLELNPGRRRTTLVVENAGDRPIQVGSHY
                     HFAETNAALRFDRAAARGMRLDIASGTAVRFEPGQQRTITLVELAGARTVFGFRGLVQ
                     GQL"
     gene            7242..>7690
                     /gene="ureC"
                     /locus_tag="VIN75_08750"
     CDS             7242..>7690
                     /gene="ureC"
                     /locus_tag="VIN75_08750"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY8974293.1"
                     /translation="MPTIGRRAYAEMFGPTVGDRVRLADTNLVIEVERDCTLAAGGYG
                     EEVKFGGGKTIRDGMGQGQAINGPGPAEAVDCVITNALIVDHWGIVKADVAIKNGLIA
                     GIGKAGNPDVQPGVDIVIGPGTEIIAAEGLIVTAGAIDTHIHFICPQ"
BASE COUNT         1152 a         2711 c         2694 g         1133 t
ORIGIN      
        1 ttcttttcag ggacgttctt cttgcccttg ttctcgggga tgtagtcgct ccacggatcg
       61 gcctgcaccg ggcccggcgt cttccacacc acgttgaact ggccgtcggc cttgacctcg
      121 ccgatgaaga cgggcttgtg caggtggtgg ttcttcgggt ccatctccag cacgaagccg
      181 ctgggcgcct tgaaggtctg gccggccatg gccgcgatca ccttgtcggt gtcggtcgac
      241 ttggccttct ccactgcctg ggcccacatg tggatgccga cgtaggtggc ttccatcggg
      301 tcgttggtca gcggcttgtc cttgtcgccg gccagattgt tcttcttggc gtagtccgac
      361 cacgccttga tgaaggccgt gttggtcggg ttcttgaccg acatgaagta gttccacgcc
      421 gccaggttgc ccaccagcgg cttggtgtcg acgccgcgca gttcctcctc gccgaccgag
      481 aacgcgacga cgggcacctc ggtcgccttc acgccctggt tgccgagctc cttgtagaac
      541 ggcacgttgg agtcgccgtt gatcgtggag acgaccgccg tcttcttgcc ttcgctcgag
      601 aacttcttga tcttggcgat gatggactgg tagtcgctgt ggccgaaggg cgtgtactcc
      661 tccatgatgt cggcgtcggc gacgcccttg ctgtggagga aggcgcgcag gatcttgttg
      721 gtcgtgcgcg ggtagacgta gtcggtgccc agcagcacga agcgcttggc ccctccgccg
      781 tccttggaca tcaggtactc caccgccggg atggcctgct ggttgggcgc ggcgccggtg
      841 tagaagacgt tcttggacag ctcctcgccc tcgtactgca ccggatagaa cagcaggctg
      901 ttcagctcct cgaacacggg cagcaccgac ttgcgcgaca cgctcgtcca gcagccgaag
      961 acgacggcga ccttgtcctg cgcgatcagt tgccgcgcct tctcggcgaa cagcggccag
     1021 ttggaggccg ggtcgaccac gaccggctcg agttgcttgc cgaggacgcc gcccttggcg
     1081 ttgatctcgg cgatggtcat gagcgcggtg tccttgagga ccgtctcgga gatggccatc
     1141 gtgcccgaca gcgagtgcag gatgccgacc ttgatcgtgt cggcggccag cgcgccgctc
     1201 gaggcgacgg cggccgcggc agcgaccagg gacaggttga agaggcggcg tgaaacggtc
     1261 atcgcggaat ccctcctggg atgtgggttg gcgtggctcg ggaagctgac ctccctgtgc
     1321 gggaggcgat ggctcgacga tagggatgca tgcgccgggc gggaatacga catgtgccgt
     1381 acgtcgctac cggcgcatca cctccggcaa cgtcccgagc acgcgcgacg ccgccgccgt
     1441 gcgcgtctcg acacccagct tctcgaagac gcgctcgagg tgcttggtga ccgtcttggg
     1501 gctcgttccc aggatctcgc cgacatcccg gttggtcttg ccctgcgcga cccaatagag
     1561 cacctccgcc tcgcgcgccg tgagccggaa ccgcagggcc agcgcctcga tcgcggtgcc
     1621 gttgtcgctc tcggtgagca cgaccagcca ctcgtcctcg gcgctcgcgg gatgcagggt
     1681 gcactgcagg cgacggccgc cggcgatcga gctccagtgc ggcggcaccg tgccggcatc
     1741 gccggccgcc agccgcgcgt ggacccacgc gcacaggccg gcaggtgccc gcaccgcgcc
     1801 gtcgagcgcg ccgccgaagt ggcgttccat cagctctcgc gcgagcggcg tccgccagag
     1861 cacccgtccg tcgcgcggcc gcaatgccat ggaggcgtgg ccgaacgcat cgagcgcatt
     1921 gcgcgcctgc gtggcgtggc gggcgctgcg caggtgggcg gcgatgcggg cgacgacctc
     1981 gcgcggtcgg atcggcttgg gcacgtagtc gacgccgccg gccgcgaagg cggcgagcac
     2041 gtgctcggtg tcggtcagcg cggtcatgaa gatgatcggg atgggcgcga tggactggtc
     2101 ggccttcatg cggcgggcga cttcgaagcc gtccatgccg ggcatgagcg cgtccagcag
     2161 cacgatgtcg ggttgcgcct gcgccgcgcg cgcgatcgcg ctctggccat cggtggccac
     2221 cagcacggtg tagcccgatt cgtcgagcgc gtcgtgcagg acggccaggt tgtcgggtac
     2281 gtcgtcgacg atgagcacgc gcccggcccc ggcgctgccc cagccgtccg ccgaggagga
     2341 caccgcctca gccaaggccg gcctcccgtt cccgcagcgc ctcggccagc accgcctcca
     2401 tcgcgtccag ccggaactgc cgtgccatcg cacgcagccg gccggtgaac gccgcgcagg
     2461 ccggttccgt ggcgtcgaca tggtcgagcc agcgatggat gccgcgcggg tagcccaggc
     2521 gcacctgctc ggacagcgcg cgcagcgcct cgatgaccgg gagagcctcg gcctgcggcg
     2581 cggtcgcttg gcgcgcggtc gcctgcggat cggccgtctc atgcgcggga tcgacggcgg
     2641 gatcggtaca ccagtcgaca gcgagcgcgc gtccgagcca gtcgagcagg tcatcggcgc
     2701 gcaccggctt gaggacgtaa tcgtcggctt gcaggcccag gtcattgtcc aggcgccggt
     2761 cgaaggcgtt ggccgagacg atggcgaccg gcgcgcggct cagccccagg gcgcgaagcc
     2821 ttcggatcgt ctcccagccg tcgatgccgg gcatcgcgag atccatcagg atggcgtcgg
     2881 gcctggcgcc ctggcgcagc cgtgcgatcg cgtcctcgcc cgagctcgcg agctccacct
     2941 cgaatcccag cggcacgagc aattggcgca gcagttcgcg atcgacctgc tcgttgtcta
     3001 cggccagcac gcgccggcgg tcgccgcgat agccgacgcg ccggggcggt gacgcgcagg
     3061 acgccgccgc ggcagactgc gcgggcagga acagccggat gcggaatgcc gtgcccgagc
     3121 caggcgtgct gcgcaccgtc atctcgccgc ccatgacatc ggtcagcatc ttggcgatcg
     3181 tcaggcccag gccggtgccc gggcccttgc catcggacgc cgccgaacct cgcgcgaacg
     3241 gctcgaagac gcgctccagg tcggtcgccg cgatgcccgg gcccgtgtcc tcgatgtcga
     3301 actgcgcgat ctcgcgctcg tggcggacgc gcaggcgcac ctcgccgcgg tcggtgaact
     3361 tcacggcgtt gcccagcacg ttgatcagca cctggcgcag cttgcgctcg tcggttcgca
     3421 ccgcgcgcgg cagcccggcg tcgaactcgg cggcgaagtc gagtcccttg gcgcgggcct
     3481 gcagcgcgaa catgtcggcg agctcgtcga gcaactggcg cagcgacacg ggagcgatcg
     3541 ccagcgagac cttgccgccc tcgatgcggg cgaggtcgag cgtgccctcg atcagcgaca
     3601 gcaggtgctc gccgccgcgc cggatcacgc tcacggcctg gcggcggtgc gcgggcaatg
     3661 ccgggtcgtc gccgagcagt tgcgcgtagc cgatcacgct gttcagcggc gtgcgcagct
     3721 catggctcag cgccgagatg tagcggctct tggcctggtt ggcggcatcg gcggcggcct
     3781 tggcaagctg caacgcggcg tcggtgcggc gatgcgaatc gatctccagg cgcagcaatg
     3841 ccgtctgctc gccgagcgcc tgcgtctggc ggttgctctc ttcctgggcg acgcgccggc
     3901 tctgccgcgc gagcaccgcc caccagccgg caacgccgcc ggccagcagc agcacggcga
     3961 acacgcccac gcaggaacgc cgcagggtgc cggcggctgc gggcaagtcg cccaaggcct
     4021 gcagttcctg ccgcagcagc agcgaacaga ccgtcgccag cagcgccgcg accggaagca
     4081 tcagcgcaag ccaggccccc agtccgccgt cgaacgccgg ccacagacgc cgcggcagca
     4141 gcgcgtgcgc ggcgtcgcgg gcctgcgtgg ccaggcgcgc gtgcggcttg cacaggtcgc
     4201 cgcagcgcgc atcgagcgag cagcacagcg agcagatcgc tccctggtag gccgggcagt
     4261 gcgcgatgtc cgcctgctcg tactcgcgct cgcagatgca gcagcgccgc atgcggggtt
     4321 gcgcggccgc cggcgccgcg gccgctgccg ggtggacggg aatgggcgga tcgtccggcc
     4381 ggcgctcggc ggcatcgcct tgctcatagg tccgtgccag gtagtagcgc cccttcgtcg
     4441 cccaggcgat caacggcgag gccgtgaatg ccgccaccag tgagatgagg gccgagaacg
     4501 cctgggccag cggcccgaac gcgcccaggt gcgccgcgat gcccagcacc gaggcgatgc
     4561 ccatcgcgcc gacgcccacc ggattgacgt cgtacaggta ggcgcgccgg aactcgatgc
     4621 ccttcggcga caaacccagg ggcttgttga ccaccaggtc cgccaccacc gccatcagcc
     4681 aggagatcgc gatgttggcg tacagcccca gcacctggcc gagcatcgtg aagacgtcca
     4741 gttccatcag catcagcgcg atggcggtgt tgaacgcgac ccagacgacg cgccccgggt
     4801 ggctgtgcgt gaggcgcgag aagaaattgg accaggcgag cgagccggcg tacgcgttcg
     4861 tcacgttgat cttgagctgc gacagcagca cgaagacggt cgtggcggcc acggccgccg
     4921 cgggccggct gaagacggtg tcccaggcgg cgaggtacat ctggttcggg tcgaccgcac
     4981 ggtcggccgg caccgagtga ccgatcgcga tccatgcgag cagtgcgccg cccagcatct
     5041 tcaacacgcc gggaagcacc cagccgggcc cgccgatcgc cacgctcgtg aaccagcgcc
     5101 gccggttggc cgccgtgagc gcgggcatga agcgcaggta gtccgcctgc tcgcccatct
     5161 gggtcatcag cgcgatgccc accgtcgtcg cggccccgaa ccgcagcatg tcgaacgttc
     5221 cgccgccgcc atcctggccc gcatagcgcc acaggccggc gagggcctgc ggattggcgt
     5281 ggaatacgaa caggtacggc aggaccagca atgcgagcca cagcggctgc gtccacacct
     5341 gcaaccgccc tatggcagtg acgccatgcg cgaccagcgg gagcacggcc agcgcgcagg
     5401 cgaggtagcc ccaggccggc ggaatgccga acgccagctc caatgcgtag gccatcaccg
     5461 cggcctcgag cgcgaagaag atgaacgtga agctcgcgta gaccagcgag gtgatcgtcg
     5521 aaccgaggta gccgaagccc gcgccgcgcg tgagcaggtc catgtccagg ccatggcggg
     5581 cagcgtgcag ggcgatcggc cagcccgcca gcgcgatcac gaagccggtc gccaggatcg
     5641 cccagaacgc gttgacgaag ccggcctgca ccagcaacgt gccgcccacc gcctcgagca
     5701 cgaggaagga ggccgccccg aacgccgtgt tggcgacccg cgcttcggac cagcggcgga
     5761 acgactgcgg cgtgtagcgc agggcgtagt cctcgagcga ttcccgcgcg acccagctgt
     5821 tgtagtcgcg gcgcaccttg accacgcgct gcggcgccgg cgcaaggctg tcgggggcgg
     5881 cgtcggtgga ggtcatcggg ccggtcgcat tcaagacccg tgccgccccg cttgcgcatg
     5941 gcaccaaacc tgcaataggc tggcgcaatg aacctcacgc cgcgcgaaaa ggacaagctc
     6001 ctgctgttca ccgccgccct gctggccgag cgccgcaagg cgcgcgggct caagctcaat
     6061 cacccggagg cgatcgcgct gatcagtgcg gcgatcctcg aaggcgcgcg cgacggccgc
     6121 agcgtggccg agctgatgag cgaggggcgc cgcgtgctca cgcgcgccga cgtgatgccc
     6181 ggcatcgccg agatgattcc cgagatccag gtcgaagcca ccttccccga cggcaccaag
     6241 ctggtgaccg tgcaccaacc gattccatga gcgcgcgcga ccgcaccaca tcggcgcacg
     6301 ccgaccgaca cgacacgagg acgacatgac cttgacgaat tcccggcacc cccttcgcat
     6361 cgccctgtcc ctcgcgtcgt gcgccctgcc gatcgccgcc atggcgcatc ccggctccct
     6421 ggccgaacac gccgcccggt cgcatggctt catcgacggc ttcgcgcatc cgttcaccgg
     6481 cctcgaccac ctgggtgcga tgctcgcggt cggcctctgg agcgcccgtt cgttgcggcg
     6541 agcctggatc gcgccggccg cgttcgtcgc ctgcctgctc gcgggcgcgc tggccacatc
     6601 gaatggcttg gccgtggctg gcgcggaacc gatgatcgcc gcatcgctgc tggtgatcgg
     6661 cctgctgctg gccgcgcgtg ccgcgctccc ggcggcggcg gccgcggcgg tcgtcggcgg
     6721 cttcgcgttc ttccacgggg cgtcgcacgg cgtggaactg ggcgctggtc cggcgctcgc
     6781 agggatggtg ctcgcgacgg cgctgctgca cctggccggc atcgcgatcg gcctcgcctt
     6841 gcgcgggcgc agcgcctggt ggactcgcat cgccggcgcg ggcctcgcga tgttcggcct
     6901 cgtgctgctg ggtgcaggcg cctgatggcc agcccggtcc atcccggcga gctgatcgtc
     6961 gacggccccg atctcgagct caacccgggc cgccggcgca cgacgctggt ggtggagaac
     7021 gccggcgacc gcccgatcca ggtcggctcc cactatcact tcgccgagac gaatgccgcc
     7081 ctgcgcttcg accgcgcggc cgcacgcggg atgcgcctgg acatcgcctc gggcaccgcc
     7141 gtgcgtttcg agccgggcca acaacgcacc atcacgctcg tggaactcgc cggcgcacgc
     7201 accgtcttcg gcttccgcgg cctggtccaa ggacaactct gatgccaacg atcggccgcc
     7261 gtgcctacgc cgagatgttc ggccccaccg tcggcgaccg cgtgcggctg gccgacacga
     7321 acctcgtgat cgaggtcgag cgcgactgca cgctggccgc cggcggctac ggcgaggagg
     7381 tcaagttcgg cggcggcaag acgatccgcg acggcatggg ccagggccag gcgatcaacg
     7441 ggccggggcc ggcagaggcg gtggactgcg tgatcaccaa cgcgctgatc gtcgaccact
     7501 ggggcatcgt gaaggccgac gtcgcgatca agaacggatt gattgccggc atcggcaagg
     7561 ccggcaaccc ggacgtccag cccggcgtgg acatcgtgat cggcccaggc accgagatca
     7621 tcgcagccga gggcctgatc gtgacggcag gagcgatcga cacccacatc cacttcatct
     7681 gcccgcagca
//
LOCUS       DATCRO010000323         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1038463, whole genome shotgun sequence.
ACCESSION   DATCRO010000323 DATCRO010000000
VERSION     DATCRO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038463"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VIN75_08755"
     CDS             <1..1078
                     /locus_tag="VIN75_08755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8974294.1"
                     /translation="PPPAPTPAPTPLTVTSTNVTTSTAGGPAVLTVTGTGLDSTLDVT
                     SSTCTNIVRSAASTSTTALYQCTTTDYTGSIVVSSAAGTALSTATFAIPAPSVTSSSV
                     SATRYGAPVLLTLTGANLDAGISVTAPGCKGLTLSTTAPNASSATTAYYTCTASGAFN
                     SSFTVKGAFGEALGSATLTVPVPIVTMAVSGDGVSGDLVFTLRGDKAPTTVDNFLAYV
                     NSGFYAGTIFHRVVPNFVVQAGGYTSATSGTLGTPKTATYAPIPLEVTGGSNVQWTAA
                     MARTSAPASATSQFFINLVDNSSFLDASQASPGYAVFGDVTASASLVQAIVAAPHCTA
                     LTGISDGSCVPIPNIVITSATQTQ"
     gene            complement(1146..2273)
                     /gene="tgt"
                     /locus_tag="VIN75_08760"
     CDS             complement(1146..2273)
                     /gene="tgt"
                     /locus_tag="VIN75_08760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY8974295.1"
                     /translation="MLKFEILGTEAQARRGRLTLNHGVVETPVFMPVGTYGTVKGVLP
                     RSLEEMGAQIILGNTFHLWMRPGLDVMAQFGGLHRFEGWKAPILTDSGGFQVWSLGEM
                     RKISEEGVRFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTPYETKGVLTTERE
                     ARLSMELSLRWAARCRDEFARLENPNALFGIVQGGMFESLREFSLDALVAMDFPGYAI
                     GGVSVGEPKEDMQRLVAHTPHRLPAHKPRYLMGVGTPEDLVHGVAHGVDMFDCVMPTR
                     NARNGHLFTRFGDLKVRNARHKADESPVDATCSCYTCGHFSRAYLHHLDRCGEMLGPM
                     LNTIHNLHYYLNLMREVRASLDEGRFAEFRVQFARDRARGI"
     gene            complement(2379..2708)
                     /locus_tag="VIN75_08765"
     CDS             complement(2379..2708)
                     /locus_tag="VIN75_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974296.1"
                     /translation="MQVRQVVAAALLAVTAVGAMSQELDPSETLQARNIAAAQAATAA
                     RTRNSVVAETHNLRADGQLSAVGERADAPVVAAVPDAQAGRTRADVKAELAQWRQSHK
                     LVTGERG"
     gene            2891..3838
                     /locus_tag="VIN75_08770"
     CDS             2891..3838
                     /locus_tag="VIN75_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974297.1"
                     /translation="MDQLRAMRVFSRVIDAGGFAKAAVAMDIAPAVATRLVAELEEHL
                     GARLINRTTRRLALTDVGEEYLERVRSILSEVDDAEAVAGLAAAEARGRLSVLSPPGF
                     TVHQLAKHLPRFRTLHPKVTLDLTVSPSVEAPDENHDVTIIVQRARPIEGAFIARRIA
                     TAEVVLCATPEYLDQHGRPKHPRDLAEHDTLMPIPASEQRELTFRPAWGGGDPAVVEL
                     RKLPPLMTTHSDTLYAAALAGLGIAGLPSFMAEDALLEQALERVLPQWHMQTVTLFAA
                     MPTRKHVPMRTRAFVDFLVATFGQNQRDPWLASAGCESC"
     gene            complement(3937..5043)
                     /gene="queA"
                     /locus_tag="VIN75_08775"
     CDS             complement(3937..5043)
                     /gene="queA"
                     /locus_tag="VIN75_08775"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY8974298.1"
                     /translation="MTRDAVHLPPPSGGFRLGDFDFELPDELIAQHPAAERTGSRLLD
                     GTATPFVDRVFREFPGLLRAGDLLVFNDTRVIKARLFGTKATGGGVEVLVERVLPPGE
                     AWAHIRASKSPRAGTTLTFGDAAAGTAFGVEVLGRCGPDDSMFHLRFPGDVFALLERH
                     GHVPLPPYIAHADEADDVRRYQTVFADRPGAVAAPTAALHFDEALLEQIAVRGVHSAR
                     VTLHVAAGTFQPVRSEDLGEHKMHTEWFDVPQVTVDAVLRTRAAGGRVVAIGTTTVRA
                     LESAAVAAEQAGHPGTLRAGADETRIFITPGYRFRVVDRLVTNFHLPKSTLMMLVSAF
                     SGMERIRALYAHAISQRYRFFSYGDAMLLDRIDG"
     gene            complement(5072..5200)
                     /locus_tag="VIN75_08780"
     CDS             complement(5072..5200)
                     /locus_tag="VIN75_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974299.1"
                     /translation="MRLIARFVVLMVAVFGVSLAFAWAWDKSDKPHDATAAAHAAP"
     gene            5202..>5391
                     /locus_tag="VIN75_08785"
     CDS             5202..>5391
                     /locus_tag="VIN75_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974300.1"
                     /translation="MDCKSCEFRGAGRYGERPPWAAGDIVRQLADQVGIGMYAPVLNR
                     SNPPVRLAWKQHGRRENRG"
BASE COUNT          815 a         1883 c         1897 g          796 t
ORIGIN      
        1 cccgccgccc gccccgacgc cggcgccgac gccgctcacc gtcacgagca cgaacgtcac
       61 caccagcacc gccggcggtc ctgccgtgct gaccgtcact ggcaccggcc tggactcgac
      121 cctcgacgtc acttcgagca cctgcaccaa catcgtccga tcggcggcga gcacgtcgac
      181 gaccgcgctg taccaatgca cgacgaccga ctacacgggc agcatcgtgg tgtcgtccgc
      241 ggccgggacg gcgctgtcca ccgccacctt cgccatcccc gcgccgtcgg tgaccagcag
      301 cagcgtcagc gcgacgcgct acggcgcacc ggtgctgctg acgctgaccg gcgcgaacct
      361 ggacgccggc atctcggtga ccgccccggg ttgcaagggc ctcacgctgt cgaccacggc
      421 gcccaacgcc agctccgcga ccaccgcgta ctacacctgc accgcgtcgg gcgccttcaa
      481 cagctcgttc acggtcaagg gggcgttcgg cgaagccctg ggctcggcaa cgctcacggt
      541 gccggtgccg atcgtgacga tggcggtctc cggcgacggc gtcagcggcg acctcgtctt
      601 cacgctgcgc ggcgacaagg cgccgaccac ggtcgacaac ttccttgcct acgtgaactc
      661 gggcttctac gcgggcacca tcttccaccg cgtcgtgccc aacttcgtcg tgcaggccgg
      721 cggctacacg tcggccacgt cgggcaccct gggcacgccc aagaccgcga cctacgcgcc
      781 catcccgctg gaggtgaccg gcggcagcaa cgtgcagtgg acggcggcca tggcgcgcac
      841 gagcgctccg gcctcggcga cctcgcagtt cttcatcaac ctggtcgaca actcgagctt
      901 cctcgacgca tcgcaggcgt ccccgggcta cgccgtcttc ggcgacgtga ccgccagcgc
      961 gtcgctggtg caggcgatcg tcgcggcgcc gcactgcacc gcactgactg gcatcagcga
     1021 cggctcgtgc gttccgatcc ccaacatcgt gatcacgtcg gcgacgcaga cgcaatagga
     1081 cgcgtcgttc gaggcacggg cgggccggct tcggccgccc ttgtcgttgc cggggcctgc
     1141 ggaccctaga tgccgcgcgc gcgatcgcgt gcgaactgca cccggaactc ggcgaagcgg
     1201 ccttcgtcga gcgacgcgcg gacctcgcgc atcaggttca ggtagtagtg caggttgtgg
     1261 atggtgttga gcatcgggcc cagcatctcg ccgcaacgat cgaggtggtg caggtaggcg
     1321 cggctgaaat ggccgcaggt gtagcagctg caggtggcgt ccaccggcga ctcgtcggcc
     1381 ttgtggcgcg cgttgcgcac cttcaggtcg ccgaagcgcg tgaacaggtg gccgttgcgc
     1441 gcgttgcgcg tgggcatgac gcagtcgaac atgtccacgc cgtgcgccac gccgtggacg
     1501 agatcctccg gcgtgcccac gcccatcagg tagcgtggct tgtgcgcggg caggcggtgg
     1561 ggcgtgtgcg ccaccaggcg ctgcatgtcc tccttgggct cgcccacgga cacgccgccg
     1621 atggcgtagc cggggaagtc catcgccacg agcgcatcga gcgagaactc gcgcagcgac
     1681 tcgaacatgc cgccctgcac gatgccgaac agcgcgttgg ggttctccag ccgcgcgaac
     1741 tcgtcgcggc agcgcgcggc ccagcgcagc gacagctcca tcgacagccg cgcctcgcgc
     1801 tcggtggtca gcacgccctt ggtctcgtac ggcgtgcatt cgtcgaactg catgacgatg
     1861 tcggagttca gcaccgtctg gatctgcatg ctgatctcgg gcgtgaggaa cagcttgtcg
     1921 ccgttgacgg gcgaggcgaa gcgcacgcct tcctccgaga tcttgcgcat ctcgccgagc
     1981 gaccacacct ggaagccgcc ggagtcggtc aggatcggcg ccttccagcc ctcgaacctg
     2041 tgcaggccgc cgaactgcgc catcacgtcg aggccgggcc gcatccacag gtggaaggtg
     2101 ttgcccagga tgatctgcgc gcccatctcc tcgagcgagc gcggcagcac gcccttgacg
     2161 gtgccatagg tgcccaccgg catgaagacg ggggtctcga cgacgccgtg gttgagcgtc
     2221 aggcggccgc ggcgggcttg ggcctcggtg cccaggatct cgaatttcaa catccccgca
     2281 ttgtcgcccg ggcaaggaga acgggcggtg cgtttcggct cgcaccgccc gtcgggcatt
     2341 cctcatcggg cggacgcgcg cggcgtccgc ggcggcgatc agccgcgttc gccggtcacc
     2401 agcttgtgcg actggcgcca ttgggccagt tcagccttga cgtcggcgcg cgtgcggccc
     2461 gcctgcgcat ccggcaccgc ggcgaccacg ggcgcatcgg cgcgctcgcc gacggcgctc
     2521 agctgcccgt cggccctcag gttgtgcgtc tcggcaacga cggaattgcg ggtgcgggcg
     2581 gcggtggcgg cctgcgccgc tgcgatgttc ctggcctgca gcgtttcgct cgggtcgagt
     2641 tcctgcgaca tggcgcccac ggcggtgacg gcgaggaggg cggcggcgac gacttgacgg
     2701 acttgcatga tgaactttct tgtctacacg gtttgaggat gtccggcggc ttgccttgcg
     2761 gggcttccgg ccgggcctcg gtgaagtggt gtatgtctgc gcttcatcga tggcttcact
     2821 gtattccgcc gatctttgtg gataaagaag acgccataga attgtttatt cccgattcag
     2881 gaagaattaa atggatcaac tgcgcgccat gcgggtgttc tcccgcgtca tcgatgccgg
     2941 cggcttcgcc aaggccgccg tggcgatgga catcgcgccg gccgtggcca cgcggctggt
     3001 ggcggagctc gaggagcatc tcggtgcgcg actgatcaac cgcaccacgc gccgcctggc
     3061 gctgaccgac gtcggcgagg agtacctcga gcgcgtgcgc agcatcctca gcgaggtgga
     3121 cgacgccgag gccgtcgcag gcctggccgc cgccgaggcg cgcgggcgcc tgagcgtgct
     3181 gagcccgccc ggcttcaccg tgcaccagct ggccaagcac ctgccgcgct tccggacgct
     3241 gcacccgaag gtgacgctgg acctcaccgt cagcccctcc gtcgaggcgc ccgacgagaa
     3301 ccacgacgtc acgatcatcg ttcaacgcgc gcggccgatc gaaggcgcgt tcatcgcgcg
     3361 gcgcatcgcg acggcggagg tcgtgctgtg cgcgacgccg gaatacctcg accagcacgg
     3421 ccgcccgaag catccacgcg atctggccga gcacgacacg ctgatgccga tccccgcgag
     3481 cgagcagcgc gagctgacgt tccggcccgc gtggggcggc ggcgacccgg ccgtggtcga
     3541 gctgcgcaag ctgccgccgc tgatgacgac gcacagcgac acgctctacg cggccgcgct
     3601 ggccggcctg ggcatcgccg gcctgccgtc cttcatggcc gaggacgcgc tgctcgaaca
     3661 ggcgctggag cgcgtgctgc cgcagtggca catgcagacg gtgacgctgt tcgccgcgat
     3721 gcccacgcgc aagcacgtgc ccatgcgcac gcgcgcgttc gtcgatttcc tggtggccac
     3781 cttcggccag aaccagcgcg acccgtggct ggcgtcggcc gggtgcgaga gctgctgagg
     3841 cgcaggcggg tcagcccgtg gcgtcacccg cgtgaccgcg cagcgcccag ccggcggatg
     3901 gcgggtcact cccgcggcca gccccgccgt gcgcgctcaa ccgtcgatgc gatcgagcag
     3961 catcgcgtcg ccatagctga agaaacggta gcgctgcgag atcgcgtggg cgtacagcgc
     4021 gcggatccgc tccatgcccg agaacgcgct caccagcatc atcagcgtgc tcttgggcag
     4081 atggaaattg gtgaccagcc ggtcgacgac ccggaagcgg taacccggcg tgatgaagat
     4141 gcgcgtctcg tcggccccgg cccgcagggt gcccgggtgg ccagcctgct cggcggcgac
     4201 cgccgcggat tcgagcgcgc gcacggtggt cgtgccgatc gcgaccacgc gtccgccggc
     4261 ggcccgggtg cgcagcacgg cgtcgacagt gacctgcggc acgtcgaacc actcggtgtg
     4321 catcttgtgc tcgcccaggt cctcgctgcg caccggctgg aaggtgccgg cggcgacgtg
     4381 cagcgtgacg cgcgcggagt gcacgccgcg gacggcgatc tgctccagca gcgcctcgtc
     4441 gaaatgaagc gcggcggtcg gcgccgcgac cgcgccgggg cggtcggcga acaccgtctg
     4501 gtagcggcgg acgtcgtcgg cctcgtcggc gtgcgcgatg tagggcggca ggggcacgtg
     4561 gccgtgccgc tcgagcagcg cgaagacgtc gccggggaag cgcaagtgga acatcgagtc
     4621 gtccgggccg cagcggccca gcacctcgac cccgaacgcc gtgcccgccg cggcgtcgcc
     4681 gaaggtcagc gtggtgcccg cccgtggcga cttgctggcg cggatgtgcg cccaggcctc
     4741 gcccggcggc agcacgcgct cgaccagcac ctcgacgccg ccgcccgtcg ccttcgtgcc
     4801 gaacagccgg gccttgatga cgcgggtgtc gttgaagacg agcaggtcgc cggcccgcag
     4861 cagcccgggg aattcgcgga agacgcggtc gacgaacggg gtggccgtgc catcgagcag
     4921 ccgcgagccg gtgcgctcgg cggccggatg ctgcgcgatc agctcgtcgg gcagctcgaa
     4981 gtcgaagtcg ccaagacgaa agccgcccga aggcggcggc aggtggacgg catcgcgggt
     5041 catcgcgacg ttttcagggg acgggcgccg ctcagggggc ggcgtgcgca gcggcggtgg
     5101 cgtcgtgcgg cttgtcggac ttgtcccagg cccaggcgaa ggccaggctc acgccgaaga
     5161 cggcaaccat caggacgaca aaacgggcaa tgaggcgcat gttggactgc aagtcctgcg
     5221 agtttcgcgg cgcaggacgt tatggagaaa gaccgccgtg ggcggcaggc gatattgtca
     5281 ggcaactggc agaccaggtg ggtatcggca tgtacgcgcc tgtgctgaat cgttcgaatc
     5341 cgcccgttcg gctcgcatgg aaacaacacg gcaggcgcga gaatcgcggc c
//
LOCUS       DATCRO010000324        11389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_824978, whole genome shotgun sequence.
ACCESSION   DATCRO010000324 DATCRO010000000
VERSION     DATCRO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824978"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1256)
                     /locus_tag="VIN75_08790"
     CDS             complement(228..1256)
                     /locus_tag="VIN75_08790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY8974301.1"
                     /translation="MNEQTLKATAAAMTADGKGLLAMDESNPTADKRFAALGIPQTPE
                     MRRAYRDMIVTAPGLSDAISGAILYDETIRSTTFTDTPFAQALEKAGIIPGIKVDGGT
                     RPLALHDGEVITEGLDGLRDRLAAYAKMGARFAKWRAVITIDDARNAPSRGCIEANAH
                     ALARYAALCQEAGIVPIVEPEVLMDGGHTLARCAEVTERTLHIVFDALRTQRVLLEGM
                     VLKPNMVVPGLKCPQQASVDDVADATVEVFRRVVPAAVGGVAFLSGGQSAQDASARLN
                     AMHVRHVDLPWPLAFSFARAIQDPAMKAWAGRKENVGAAQKALVERARLNRLARRGEY
                     TVAMETPR"
     gene            1484..2497
                     /gene="gnd"
                     /locus_tag="VIN75_08795"
     CDS             1484..2497
                     /gene="gnd"
                     /locus_tag="VIN75_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY8974302.1"
                     /translation="MQLGMIGLGRMGANMARRLMSQGHECVVQDPDPDAVARMVSAGA
                     TGAATLAEMAAKMARPRAVWMMVPAAVVDHVIDDLVAHLEPGDILIDGGNSWYRDDID
                     RAARLKEKGLHYVDCGTSGGIAGADRGYCLMVGAEQDIFDHLTPIFSALAPGVDAAER
                     TVGRTGEPTNAEQGFLHCGPNGAGHFVKMVHNGIEYGMMAAFAEGFNILRHANVGKQD
                     HAKDAETAPMRDPQYYQFDIDVPQVAEVWRRGSVVGSWLLDLTAKALVADPQLSKFGG
                     RVSDSGEGRWTIQAAIDEGVPTHVLSAALYDRFSSRGEATFANQILSAMRAEFGGHAE
                     KKA"
     gene            2615..4015
                     /gene="zwf"
                     /locus_tag="VIN75_08800"
     CDS             2615..4015
                     /gene="zwf"
                     /locus_tag="VIN75_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8974303.1"
                     /translation="MSDALVLFGFTGDLANKKIFPALYAMSKRGELVPRVVGVAGRAQ
                     SDDEVHERVRESIEQHGGGIDDGPAFDKLVHAIRYVGGDYNKRETFDALKQALQGSKR
                     PAHYLAVPPSLFETVIRSLGAAGLAKDARLIVEKPFGRDLASAKELDAVAHSVFPEDA
                     IFRIDHYLGKEAIMNLLYFRFANSFLEPLWCRDHVTSVQMTMSEDFGVEERGAFYESA
                     GALRDVVENHLFQIVALLAMEPPKSRAFADLQAAKADVFKAMRPIRPEDVVRGQYDGY
                     RQEKDVAPDSDVETFVALRLFVDTPRWQDVPFFLRAGKELPTTAVEVQIQFKPPAQVL
                     FEDAGDRPNYLRFRLQPSSSIALAVRVKDVGKKFVGTQRELYACDDLAGEEAPYDRLL
                     GDAMAGDGSLFTSPEAVEAAWKAVDDVLVHHPKVVAYERGTWGPKEAEALIAGNGCWK
                     APQPEPGCGEHRKKAA"
     gene            4015..5655
                     /gene="pgi"
                     /locus_tag="VIN75_08805"
     CDS             4015..5655
                     /gene="pgi"
                     /locus_tag="VIN75_08805"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8974304.1"
                     /translation="MSESLTQRETWKALQDHCAKVSRRHLRELFDEDDGRGTRLTLEA
                     AGLYLDYSKNRVTDKTMKLLVELAHDCGLEKKIAAMFSGERINTTEHRAVLHVALRAP
                     RGQRILVDGVDVVPGVHEVLDRMAVFAGQVRDGTWRGFTGQRIRHVINIGIGGSDLGP
                     VMAYEALRHYSQRDLDMRFVSNVDSTDLVEATRGLDPACTLFIVSSKTFTTLETMTNA
                     RAARDSLVAQLGSEDAVARHFVAVSTNAEGVGRFGIAPDNMFGFEDWVGGRYSMDSAI
                     GLSTMIAIGADHFRAMLHGFHEMDEHFRSAPLAQNLPALMGLLSIWNNDFLGFSTLAV
                     LPYEQYLKRFPAYLQQLTMESNGKHVTLQGDAVKADTSPVVWGEPGTNGQHSFYQLIH
                     QGTRIVPCDFIGFCTALAPIGGQHDLLIANLIAQAEALAFGKTARQVEAEGVAPELVP
                     HRVFEGNRPSNTILADALTPETLGKLVALYEHSVFTQGAIWDIDSFDQWGVELGKQLA
                     AKVIPELQSPHDPELAHDSSTNALIRRYRERRNGALPE"
     gene            5665..7314
                     /gene="pgm"
                     /locus_tag="VIN75_08810"
     CDS             5665..7314
                     /gene="pgm"
                     /locus_tag="VIN75_08810"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY8974305.1"
                     /translation="MAQIISPLAGKPAPRSSLVDIPKLISAYYTVVPDPAEPAQRVAF
                     GTSGHRGNAFDGSFNERHVLAITQAICDHRAAQGIAGPLFIGIDTHALSEPALRSALE
                     VLAANGVDTMLAMNDEYTPTPAISHAILTHNRERTSRLADGIVVTPSHNPPDNGGFKY
                     NPTNGGPAADDITKAIQAAANAYLEKDLAGVKRTTYAKALAAPTTHRRDYLGWYVADL
                     GNVIDMAAIRDARVRLGVDPLGGAGVHYWSRIADQYRLDLTVVSDVVDPTFSFMSMDW
                     DGKIRMDPSSPYAMARLVDLKSQFDVAFACDTDHDRHGIVTRSAGLMQPNSYLAVAID
                     YLYRNRPQWGAQAAIGKTVVSSRMIDLVAQRLQRRMVEVPVGFKWFAAGLADGSFGFG
                     GEESAGAAFLRRDGTAWTTDKDGITAALLAAEITARTGRDPGAAYDEMTRKLGKPVSD
                     RVEAPADAQQKKRLSALSPEAITSKEIAGEPITEVIAKAPGNGAAIGGIRVSSRSGWF
                     AARPSGTEEIYKIYAESFEGEAHLKRLLDEAQALVDKAIAS"
     gene            7373..7813
                     /locus_tag="VIN75_08815"
     CDS             7373..7813
                     /locus_tag="VIN75_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8974306.1"
                     /translation="MKSALSLALVAAALAGGAHAVDVYKWKDSKGVVHYGDRPASGAS
                     AARVSVRDDQVDPDDAARAQQRLSETQDRLQDDAGSDEPETPPRETARPKTQADTGSC
                     AYKWRLYMQSQGCFDSNRVAGGKGVTRNGARVCPQIVQPDCPHP"
     gene            7810..9042
                     /locus_tag="VIN75_08820"
     CDS             7810..9042
                     /locus_tag="VIN75_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY8974307.1"
                     /translation="MKALRAGALSLLLLSTFAHGAPDPLAAQAVPVHVDAIAGDDFAD
                     LAPFGRAVGPARVVALDEQTHGGHEEFVLKTRLLRYLHERRGFDVLVIESGFFDVGRI
                     EQARQAGGRVDDLAPDNIFYMYSKSAEGRALLRYVDSTRATTHPLMLVGMDSQLSGVL
                     SRSRLVPELAGFLVAHHSHATESRDWPGFAATVAAMTALRSTAPPPTDRAAFERLGAQ
                     LAGELEALGDAPGAGVGSAGWWGRVVAGLRAQATQLWVAPRGNARDVAMGDNVAWIAD
                     RLVPGRRIVVWGHAIHLMHDAPRPDAPPLAGTVMHRHLGAGYHVAHLTGLEGRYLDYE
                     TLQPRAIPAAAPGSLEARLATRPGAALYLAPPKALAPDAPAARYIDYLPLGPLPGRGL
                     HWESTFFIRAITPVTMVR"
     gene            9047..9505
                     /locus_tag="VIN75_08825"
     CDS             9047..9505
                     /locus_tag="VIN75_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974308.1"
                     /translation="MTDLTIRSIAPADRAAWAPLWDGYNAFYGREGTTALAPEITEVL
                     WQRLFDADEPVHGLVAEGGGRLLGIAHFLFHRSTTRIEPVCYLQDLFTAPEARGRGVG
                     RALIEGVYARALEAGAKRVYWQTRQDNATAQRLYDQVARHHGFVVYAHDA"
     gene            complement(9523..10113)
                     /locus_tag="VIN75_08830"
     CDS             complement(9523..10113)
                     /locus_tag="VIN75_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974309.1"
                     /translation="MSKTYVVSGCGMKFDAALWPVPANVEIHFYFQCMREPQLYKTRT
                     ILDKLMLEVGMFPDRVAVAGDMIPSHFVWAHAGEQPASGVFRRQSGQLAMDLADTDAS
                     RPVALSHIVKTLSADRSRSTTAIHWIVRESEDLAELSGLDCQYPVRLRGGVADDGDES
                     REMPLSGQMPLRPSQRMPLSPMGMGPTEAWPGVRRR"
     gene            complement(10556..11386)
                     /gene="cobA"
                     /locus_tag="VIN75_08835"
     CDS             complement(10556..11386)
                     /gene="cobA"
                     /locus_tag="VIN75_08835"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY8974310.1"
                     /translation="MSDAMTVTLHPVHLVGAGPGDPELLTLKALRVLRQATVIVVDDL
                     VGEAVLDAALADAVAPPRVVRVGKRGGCASTPQEFIEKLMVREALAGERVVRLKGGDP
                     LVFGRAGEEIAALQAAGVEVEIVNGITSGLAAAGSAGIGWTDRRAGAQGVLLVTGHAG
                     HGCEEPDWDAIARVAASGVTLVVYMGVARAEAVVGRLRTALPGDLPVAIVQRASAADE
                     VIARTTLDALVDTIRSERIGSPAILVIGRVAGLAAELAHRANVVTGHRAKPRRPSSCA
                     "
BASE COUNT         1732 a         4126 c         3946 g         1585 t
ORIGIN      
        1 gagcttcacg ccgcaggcgt cgatgcgggt gcagctggga gcgcccgcgg ggcggccggg
       61 gtttccgtga gcgccgagcc cgcgtcgtca gccccatagc ctgtcatcct gcgcgaagtc
      121 gcgggatcac cccaccccgg cgagaacgct tccttcacga ggcgtggatc ctgcgacttc
      181 gcgcaggatg acggctcttg gtaccttggg cggactgacg atgtcagcta gcgcggcgtc
      241 tccatcgcca ccgtgtactc cccgcgccgc gccagccggt tcagccgcgc ccgctcgacc
      301 agcgccttct gcgccgcgcc gacgttctcc ttcctcccgg cccacgcctt catcgcggga
      361 tcctggatcg cgcgggcgaa cgagaacgcc agcggccacg gcaggtcgac atggcggacg
      421 tgcatcgcgt tcaggcgcgc cgacgcgtcc tgcgccgact gcccgcccga caggaacgcg
      481 acgccgccga cggccgccgg caccacgcgg cggaagacct cgaccgtggc gtccgcgaca
      541 tcgtcgacgc tcgcctgctg cgggcatttc agccccggca cgaccatgtt gggcttgagc
      601 accatgccct ccagcagcac gcgctgggtg cgcagcgcat cgaacacgat gtgcagcgtg
      661 cgctcggtga cctcggcgca gcgcgcgagc gtgtgcccgc cgtccatcag cacctcgggc
      721 tcgacgatgg gcacgatgcc ggcctcctgg cacagcgccg cgtagcgggc gagcgcgtgc
      781 gcgttggcct cgatgcagcc gcgtgaaggg gcgttcctgg catcgtcgat cgtgatgacc
      841 gcgcgccact tggcgaagcg cgcgcccatc ttcgcgtagg ccgcgagccg gtcgcgcagg
      901 ccgtccagac cctcggtgat gacctcgccg tcgtgcagcg cgagcggcct ggtgcccccg
      961 tccaccttga tgccggggat gatgcccgcc ttttcgagcg cctgggcgaa cggcgtgtcg
     1021 gtgaaggtcg tggagcggat cgtctcgtcg tacaggatcg cgccggagat cgcatccgac
     1081 aggcccggcg cggtgacgat catgtcgcgg taggcgcggc gcatctcggg cgtctgcggg
     1141 atgcccagcg cggcgaagcg cttgtccgcg gtgggattgc tctcgtccat cgccagcagg
     1201 cccttgccgt cggcggtcat cgccgcggcg gtggccttca aggtctgttc gttcatcgtc
     1261 gctgctcccg gttcgcgcca gtgtgcgtcg cggccaaagc ccatgttccc gcgaagaggc
     1321 gccagcccgg ccttgcggca aatcgcgcac ccgtcgcgca ggctgtcggc gggcttgcga
     1381 gctcggccat tgggcatggc gcttgccgcc cgtccggatc gcccccgttc gcgtcgactc
     1441 atccgtgcgc gatgatgacg ccactcaact tcgaaagaac aacgtgcaac tcggaatgat
     1501 cggactggga cggatgggtg cgaacatggc gcgccgcctg atgagccagg gccacgaatg
     1561 cgtggtgcag gaccccgatc ccgatgccgt ggccaggatg gtgtccgcgg gcgccaccgg
     1621 cgcggcgacc ctggcggaga tggccgcgaa gatggccagg ccgcgcgcgg tctggatgat
     1681 ggtgccggcc gccgtcgtcg accatgtgat cgacgacctc gtcgcgcacc tggagcccgg
     1741 cgacatcctc atcgacggcg gcaactcctg gtaccgcgac gacatcgacc gcgcggcgcg
     1801 cctgaaggaa aagggcctgc actacgtcga ctgcggcacc agcggcggca ttgcgggcgc
     1861 cgaccgcggc tactgcctga tggtgggcgc cgagcaggac atcttcgacc acctgacgcc
     1921 catcttcagc gcactggccc ccggcgtcga cgctgccgag cgcaccgtcg gccgcaccgg
     1981 cgagcccacg aacgcggaac agggcttcct gcactgcggt ccgaacggcg ccggccactt
     2041 cgtcaagatg gtgcacaacg gcatcgagta cgggatgatg gccgcgttcg ccgagggctt
     2101 caacatcctg cgccacgcca acgtcggcaa gcaggaccac gccaaggacg ccgagacggc
     2161 gcccatgcgc gacccgcagt actaccagtt cgacatcgac gtgccccagg tggccgaggt
     2221 gtggcggcgc ggcagcgtcg tcggctcgtg gctgctcgat ctcaccgcca aggcgctcgt
     2281 cgccgacccg cagctctcga agttcggcgg ccgcgtgtcc gactcgggcg agggccgctg
     2341 gacgatccag gccgccatcg acgagggcgt gcccacccac gtgctcagcg ccgcgctgta
     2401 cgaccgcttc tcgtcgcgcg gcgaggccac cttcgccaac cagatcctgt ccgcgatgcg
     2461 cgccgagttc ggtgggcacg cggagaaaaa ggcctgactc tccatgaacg cggacaacgc
     2521 ctcctcttcc gcgggccgag cgccgggccg ctcccaagcc ggcccgcatc ccctgggggg
     2581 atcggccgac gtacccggcg gacgaggggc cgacatgtct gacgcccttg tcctgttcgg
     2641 attcaccggc gacctcgcga acaagaagat ctttcccgcg ctgtatgcga tgagcaagcg
     2701 cggcgaactg gtgccccggg tagtcggggt cgcgggacgg gcgcagtccg acgacgaggt
     2761 ccacgagcgg gtgcgcgagt cgatcgagca gcatggcggc ggcatcgacg acggccccgc
     2821 gttcgacaag ctggtgcacg cgatccgcta tgtcggcggc gactacaaca agcgcgagac
     2881 cttcgatgcg ctgaagcagg cgctgcaagg cagcaagcgc cccgcgcact accttgcggt
     2941 cccgccgtcg ctgttcgaga ccgtcatcag gagcctgggc gcggcgggac tggccaagga
     3001 cgcccggctg atcgtcgaga agccgttcgg acgcgacctc gcgtccgcga aggagctcga
     3061 cgcggtggcg cactcggtgt tcccggagga cgcgatcttc cgcatcgacc actacctggg
     3121 caaggaggcg atcatgaacc tcctgtactt ccgcttcgcc aactcgttcc tcgagccgct
     3181 gtggtgccgc gatcacgtca ccagcgtgca gatgacgatg tccgaggact tcggcgtcga
     3241 ggagcgcggc gcgttctacg aatcggccgg cgcgctgcgc gacgtcgtcg agaaccacct
     3301 gttccagatc gtcgcgctgc tcgcgatgga gccgcccaag tcgcgcgcct tcgccgacct
     3361 gcaggccgcc aaggccgacg tgttcaaggc catgcgcccg atcaggcccg aggacgtcgt
     3421 gcgcggccag tacgacggct accgccagga aaaggacgtg gcgcccgact ccgacgtcga
     3481 gaccttcgtc gccctgcgcc tgttcgtcga caccccgcgc tggcaggacg tgccgttctt
     3541 cctgcgtgca ggcaaggagc tgccgaccac ggccgtcgag gtgcagatcc agttcaagcc
     3601 gcccgcgcag gtgctgttcg aggatgccgg cgatcgcccc aactacctgc gcttccgcct
     3661 gcagccgtcc tcgtcgatcg cgctggcagt gcgcgtcaag gacgtcggca agaagttcgt
     3721 cggcacccag cgcgagctgt acgcgtgcga cgaccttgcc ggagaggagg cgccgtacga
     3781 ccgcctgctg ggcgacgcga tggccggcga cggctcgctg ttcaccagcc ccgaggcggt
     3841 cgaggccgcg tggaaggcgg tcgacgacgt gctcgtccac catccgaagg tcgtcgcgta
     3901 cgagcgcggc acgtgggggc cgaaggaagc cgaggcgctg atcgcgggca acggttgctg
     3961 gaaggcgccg cagcccgagc ccggctgcgg cgagcatcgg aagaaggcgg cgtgatgagc
     4021 gagtccctca cccagcgcga gacctggaag gcgctgcagg atcactgcgc caaggtctcg
     4081 cgcaggcacc tgcgcgagct gttcgacgag gacgacgggc gcggcacgcg gctgacgctg
     4141 gaggcggcgg gcctgtacct ggactactcg aagaaccgcg tcaccgacaa gacgatgaag
     4201 ctgctcgtcg agctcgcgca cgattgcggc ctggagaaaa agatcgccgc gatgttctcc
     4261 ggcgagcgca tcaacacgac cgagcaccgc gccgtgctgc acgtggcgct gcgcgcgccg
     4321 cgcggccagc gcatcctggt cgatggcgtc gacgtcgtgc cgggcgtgca cgaagtgctc
     4381 gaccgcatgg ccgtcttcgc cgggcaggtg cgcgacggca cctggcgcgg cttcacgggc
     4441 cagcgcatcc gccacgtgat caacatcggc atcggcggct ccgacctcgg cccggtgatg
     4501 gcgtacgagg cgctgcgcca ctacagccag cgcgacctgg acatgcgctt cgtctccaac
     4561 gtcgactcca ccgacctcgt cgaggccacg cgcgggctcg atccggcgtg cacgctgttc
     4621 atcgtgtcgt ccaagacgtt cacgaccctg gagaccatga ccaacgcgcg ggccgcgcgc
     4681 gactcgctgg tcgcgcagct gggcagcgag gacgcggtgg cgcggcactt cgtcgccgtc
     4741 tccaccaacg cggaaggcgt cggcaggttc ggcatcgcgc cggacaacat gttcggcttc
     4801 gaggactggg tcggcgggcg ctactcgatg gactcggcga tcggcctgtc gacgatgatc
     4861 gcgatcggcg cggaccactt ccgcgcgatg ctgcacggct tccacgagat ggacgagcac
     4921 ttccgcagcg cgccgctcgc gcagaacctg ccggcgctga tgggcctgct gtcgatctgg
     4981 aacaacgact tcctgggctt ctccacgctg gccgtgctgc cctacgagca gtacctcaag
     5041 cgcttcccgg cctacctgca gcagctgacg atggagagca acggcaagca cgtcacgctg
     5101 cagggcgatg cggtgaaagc cgacacctcg cccgtcgtct ggggcgagcc cggcaccaac
     5161 ggccagcact cgttctacca gctgatccac cagggcacgc gcatcgtgcc gtgcgacttc
     5221 atcggcttct gcaccgcgct ggcgccgatc ggcggacagc acgacctgct catcgccaac
     5281 ctcatcgcgc aggccgaggc gctggcgttc ggcaagactg ccaggcaggt cgaggccgag
     5341 ggcgtcgcgc cggaactggt gccgcaccgc gtgttcgagg gcaaccggcc cagcaacacg
     5401 atcctggccg acgcgctcac gcccgagacg ctgggcaagc tggtggcact ctacgagcac
     5461 agcgtgttca cgcagggcgc gatctgggac atcgactcgt tcgaccagtg gggcgtggag
     5521 ctgggcaagc agctcgccgc caaggtcatc cccgagctgc agagcccgca cgatccggag
     5581 ctcgcgcacg acagctccac caacgctctc atccgccgct atcgcgaacg ccgcaacggc
     5641 gccttgcctg aataatccga ccccatggcc cagatcatct cgcccctcgc cggcaagccg
     5701 gccccgcgct cgtcgctcgt cgacatcccc aagctgatca gcgcgtacta caccgtcgtg
     5761 cccgatccgg ccgaacccgc gcagcgcgtg gcgttcggca cctcgggcca tcgcggcaac
     5821 gcgttcgacg gctcgttcaa cgagcgccac gttctggcga tcacgcaggc gatctgcgac
     5881 caccgcgcgg cgcaaggcat cgccggcccg ctgttcatcg gcatcgacac gcatgccttg
     5941 tccgagcctg cgctgcgcag cgcgctcgag gtgctggcag ccaacggcgt cgacacgatg
     6001 ctggcgatga acgacgagta cacgcccacg ccggccatct cgcacgcgat cctcacgcac
     6061 aaccgcgagc gcacgtcgcg gctggccgac ggcatcgtgg tgacgccgtc gcacaacccg
     6121 cccgacaacg gcgggttcaa gtacaacccg accaacggcg gcccggcggc cgacgacatc
     6181 accaaggcca tccaggccgc ggccaacgcc tatctggaaa aggacctggc gggcgtcaag
     6241 cggacgacgt acgcgaaggc gctcgccgcg cccaccacgc accggcgcga ctacctgggc
     6301 tggtacgtgg ccgacctggg caacgtcatc gacatggccg cgatccgcga cgcgcgcgtc
     6361 cgcctcggcg tcgatccgct gggcggcgcc ggcgtgcact actggagccg catcgccgac
     6421 caataccggc tcgacctgac ggtggtcagc gacgtggtcg acccgacctt ctcgttcatg
     6481 agcatggact gggacggcaa gatccgcatg gatccgtcct cgccgtacgc gatggcgcgc
     6541 ctggtcgacc tcaagagcca gttcgacgtc gcgttcgcct gcgacaccga ccacgaccgc
     6601 cacggcatcg tcacgcgcag cgccggcctg atgcagccca acagctacct ggccgtggcc
     6661 atcgactacc tgtaccgcaa ccgcccgcag tggggcgcgc aggcggcgat cggcaagacg
     6721 gtggtgtcct cgcgcatgat cgacctcgtc gcgcagcggc tgcagcgccg catggtggag
     6781 gtgcccgtcg gcttcaagtg gttcgccgcg ggcctggccg acggcagctt cggcttcggc
     6841 ggcgaggaga gcgcgggcgc cgcgttcctg cgccgcgacg gcaccgcgtg gacgaccgac
     6901 aaggacggca tcaccgccgc gctgctggcc gcggagatca ccgccaggac gggccgcgac
     6961 ccgggcgcgg cctacgacga gatgaccagg aaactgggca aaccggtgtc cgaccgcgtc
     7021 gaggcgccgg ccgacgcgca gcagaagaag cggctgtccg cgctgtcgcc cgaggcgatc
     7081 acgagcaagg agatcgccgg cgagccgatc accgaggtca tcgcgaaggc gccgggcaac
     7141 ggcgcggcca tcggcggcat ccgcgtgagc tccaggagcg gctggttcgc ggcgcggcca
     7201 tcgggcaccg aggagatcta caagatctac gccgagagct tcgagggcga ggcgcacctg
     7261 aagcgcctgc tcgacgaggc gcaggcgctg gtggacaagg cgatcgcgtc ctgaggccgc
     7321 ggcgcgcggc acaatggggg atcgcgtctc gccggcccgg agcgccgcca ccatgaagtc
     7381 tgccctctcc ctggccctcg tcgccgccgc gctggccggc ggcgcgcatg cggtggatgt
     7441 ctacaagtgg aaggacagca agggcgtcgt ccactacggc gaccgcccgg cctccggcgc
     7501 ctcggcggcc cgggtcagcg tgcgcgacga ccaagtcgat ccggacgacg ccgcgcgggc
     7561 gcagcagcgg ctgtccgaaa cgcaggaccg gctgcaggac gacgccggca gcgacgagcc
     7621 cgagacgcca ccccgcgaga ccgcgcggcc caagacgcag gcagacacgg gaagttgcgc
     7681 ctacaaatgg cggctctaca tgcaatcgca gggctgcttc gacagcaacc gcgtcgccgg
     7741 cggcaagggc gtcacgcgca acggcgcccg cgtctgcccg cagatcgtcc agccggactg
     7801 cccgcatcca tgaaggcgct gcgcgccggc gcgctgtcgc tcctgctgct gtccaccttc
     7861 gctcatgggg cgcccgaccc gctcgcggcg caggccgtgc cggtgcacgt cgacgccatc
     7921 gcgggcgacg acttcgccga cctcgcgccg ttcggccgcg ccgtcggccc cgcgcgcgtc
     7981 gtggcgctgg acgagcagac gcacggcggc cacgaggagt tcgtgctgaa gacgcgcctg
     8041 ctgcgctacc tgcacgagcg gcgtggcttc gacgtgctcg tgatcgagag cggcttcttc
     8101 gacgtcggac gcatcgagca ggcgcgccag gccgggggac gcgtcgacga cctcgcgccc
     8161 gacaacatct tctacatgta ctcgaagtcg gccgagggcc gcgcgctgct gcgctacgtc
     8221 gactcgacgc gcgcgacgac tcacccgctc atgctggtgg gcatggacag ccagctgtcg
     8281 ggcgtgctga gccggtcgcg gctcgtgccg gagctcgccg gcttcctcgt ggcgcaccac
     8341 tcgcacgcaa cggagtcgcg cgactggccc ggcttcgcgg ccacggtcgc ggcgatgacc
     8401 gcgttgcgtt cgaccgcgcc gccgccaacc gatcgcgccg cgttcgagcg cctgggcgcg
     8461 cagctcgccg gcgagctgga agccctcggc gacgcgccgg gcgcgggcgt gggctcggcc
     8521 ggctggtggg gccgtgtcgt cgcggggctg cgcgcccagg cgacgcagct ctgggtggcg
     8581 ccccgaggca acgcccgcga cgtggcgatg ggcgacaacg tcgcgtggat cgccgaccgt
     8641 ctggtgccgg gccggcgcat cgtcgtctgg ggccatgcga tccatctcat gcacgacgca
     8701 ccgaggcccg acgcgccccc gctcgccggc acggtgatgc accggcactt gggcgccggc
     8761 tatcacgtgg cgcacctgac cggcctggag ggccgctatc tcgactacga gacgctgcag
     8821 ccgcgcgcca tccccgcggc cgcacccggc agcctcgagg ctcgcctcgc gacccgcccc
     8881 ggtgccgcgc tgtacctcgc gccgcccaag gcgctcgcgc cggatgcgcc cgccgcgcgc
     8941 tacatcgact acctgccctt gggccccctg cccggccgcg ggctgcactg ggagagcacg
     9001 ttcttcatcc gcgcgatcac gccggtgacg atggtgcgct gaccgcatga ccgacctgac
     9061 catccgctcc atcgcccccg ccgaccgcgc cgcctgggcg ccgctctggg acggctacaa
     9121 cgccttctac ggtcgcgagg gcacgacggc gctcgcgccc gagatcaccg aggtgctgtg
     9181 gcagcgcctg ttcgacgccg acgagccggt gcacgggctg gtggccgagg gcggcggccg
     9241 cctgctgggc atcgcgcatt tcctgttcca ccgcagcacc acgcgcatcg agcccgtgtg
     9301 ctacctgcag gacctgttca ccgcgcccga agcgcgcggc cgcggcgtgg gccgggcgct
     9361 gatcgaagga gtctatgcgc gggccctcga ggcgggcgcg aagcgcgtct actggcagac
     9421 caggcaggac aacgccacgg cgcagcgcct ctacgaccag gtggcgcgcc accacggctt
     9481 cgtggtgtac gcgcacgacg cctgaagcgg cgccccaggc tttcagcggc ggcggacgcc
     9541 cggccaggcc tcggtcgggc ccatgcccat cggcgacagc ggcatgcgct ggctgggacg
     9601 cagcggcatc tggccggaca gcggcatctc gcgcgattcg tcgccgtcgt cagcgacgcc
     9661 gccccgcagg cgcaccggat attggcagtc gaggccggac agctcggcca ggtcctcgga
     9721 ctcgcggacg atccagtgga tggcggtggt gctgcgcgag cggtcggcgc tcagcgtctt
     9781 gacgatatgg ctcagggcca ccgggcgcga cgcgtcggtg tcggccagat ccatcgcgag
     9841 ctggccggac tggcggcgga acacgcccga ggcgggctgc tcgccggcgt gcgcccagac
     9901 gaaatggctg gggatcatgt cgccggccac ggcgacgcgg tcggggaaca tgccgacttc
     9961 cagcatcagc ttgtccagga tcgtgcgcgt cttgtagagc tggggctcgc gcatgcactg
    10021 gaaatagaag tggatctcga cgttggccgg caccggccac agcgccgcgt cgaacttcat
    10081 cccgcacccg gaaacgacgt atgtcttgct catggttcca aggcgggttc ctggcccgcg
    10141 atgccgggcc cgacgcccgt tgcctcgatt ctaggcagcg acgtgccgcc gagcatgggt
    10201 ggaaaacaac aaggtcagga catgcctcac gcccggcgga gatttttgtt caccgtcgta
    10261 acgtctgacc gtccgcggcg tgattttcta ccgcttggcg cccctggcgg cccaccgaac
    10321 gcctggaagt gtaagcgcgg agagcgcctc ccctacgttc gtgggatggg ccatatcaac
    10381 caattcgtgc tgcccagcgg ccgggcgcag cgcgcgatgc aacgccgccg catcgcgccc
    10441 gcaccaagtg tcatcctgcg cgcagtcgca ggatccacgg tggccatgaa accgcctcgc
    10501 cggggtggat cctgcgactc cgcttcgctg cgcgcaggat gacggtcgtc ggggcctaag
    10561 cgcatgatga gggtcgtcgg ggcttcgcgc gatggccggt cacgacgttc gcgcggtgcg
    10621 caagctccgc ggccaacccg gccacccggc cgatcaccag gatcgccggg ctgccgatcc
    10681 gctcgctgcg gatcgtgtcg acgagcgcgt ccagcgtcgt gcgagcgatc acttcgtcgg
    10741 ccgcactcgc acgttggacg atggccaccg gcaggtcgcc gggcaaggcg gtgcgcaggc
    10801 ggccgacgac ggcctcggcc cgcgccacgc ccatgtagac gaccagcgtc acgccgctcg
    10861 ccgcgacgcg ggcgatggcg tcccagtcgg gctcctcgca gccgtgcccc gcgtggccgg
    10921 tgaccagcag caccccctgc gcgccggcgc ggcggtcggt ccagccgatg cccgccgatc
    10981 ccgccgcggc caggccggag gtgatgccgt tgacgatctc gacctcgacg ccggccgcct
    11041 gcagcgcggc gatctcctcg ccggcgcggc cgaagacgag cgggtcgccg cccttgagcc
    11101 gcacgacgcg ttcgcccgcc agcgcctcgc gcaccatcag cttctcgatg aactcctgcg
    11161 gcgtcgacgc gcagccgccg cgcttgccga cgcgcacgac ccgcggcggc gcgacggcgt
    11221 cggccagggc cgcgtccagc accgcctcgc cgacgaggtc gtcgaccacg atcaccgtgg
    11281 cctggcgcag cacgcgcagc gccttcagcg tgagcagctc gggatcgccg gggccggcgc
    11341 cgacgaggtg gacggggtgc agggtgacgg tcatggcgtc gctcatggc
//
LOCUS       DATCRO010000325         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1038502, whole genome shotgun sequence.
ACCESSION   DATCRO010000325 DATCRO010000000
VERSION     DATCRO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038502"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIN75_08840"
     CDS             complement(<1..297)
                     /locus_tag="VIN75_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8974311.1"
                     /translation="MAEGARSPATTALAACAVAVAVLLAGCASGPRSGGGVAAGRDGP
                     GTNIPPDLANVPDAEPRVEPIRSLGGTSKPYTVLGRGYVPITDDRPFRESGLASW"
     gene            complement(290..>2395)
                     /gene="glnE"
                     /locus_tag="VIN75_08845"
     CDS             complement(290..>2395)
                     /gene="glnE"
                     /locus_tag="VIN75_08845"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY8974312.1"
                     /translation="ALEEYFQVQGREWERFAWMKSRVVAPRDIVLAGQAQALRGAVLP
                     FVFRRYLDYSVFDSLRVLHRQIREQSARRSAGRPERANDVKLSRGGIREIEFTVQLLQ
                     VVRGGQFPELRTRPTLDALQRLARANLMPQETADALAAAYEFLRRVEHRIQYLDDQQT
                     HVLPVSDDDLRWIAQSMGYASCCPFLEQLDTHREFVAQEFDKLLGGEKPCNGKCSGKK
                     AAAPADLADLLGDLPPVFAERIRDWCEQPRVLALREETRARLRQLVQRTGQWLKEPDG
                     DGAGQTPRHVDAAMRWADWIEPLMRRESYLALLVERPAVQERLLRLLGAAKWPARYLM
                     QHPGVIDELASDEMLSGRFVAAEFERELDARHASLTRTGEADEERLLNLLRHAHHAEV
                     FRTLARDVDGRLTVEQVADDLSALADATLRVTARWCWPHVRNRHREVPQFAIIGYGKL
                     GGKELGYGSDLDIVFVYDDDDERAGEVYAAYVRKLINWLTVKTREGDLFEIDTALRPN
                     GNSGLLTTSFEAYEKYQLGRGSNTAWTWEHQAMTRARFVLGSEDHGAELGARFDQVRE
                     AVLVAPRDREALKAEIIAMRNKLRSARPVKPDRFDVKHSPGGMVDAEFAVQFLVLSSS
                     ADHPELIPNVGNIALLLRAELAGLLPEGVGRSAARAYRELRRVQHRARLNEEPTQVTP
                     PALAAERDAMLALWKAVFG"
BASE COUNT          310 a          838 c          895 g          352 t
ORIGIN      
        1 ccacgaggcc agccccgatt cgcggaacgg tcggtcgtcg gtgatcggca catagccgcg
       61 gcccagcacc gtgtagggct tgctggtgcc gcccaggctg cggatcggct cgacgcgggg
      121 ttcggcgtcg ggcacgttgg cgaggtcggg cgggatgttg gtcccggggc cgtcgcggcc
      181 ggcggccacg ccgcccccgc tgcgcggacc gctggcgcag cccgcaagaa gaacggcgac
      241 ggctaccgcg cacgccgcca gcgcggtggt ggccggggaa cgggctccct cagccaaata
      301 cggccttcca cagcgccagc atggcatcgc gctcggccgc cagcgcgggc ggcgtgacct
      361 gcgtaggttc ctcgttgagg cgcgcgcggt gctgcacgcg gcgcagctcg cgataggcgc
      421 gcgccgcgct gcggcccacg ccttcgggca gcaggccggc cagttcggca cgcagcagga
      481 gggcgatgtt gcccacgttc ggaatcagct cgggatggtc ggccgaagac gacagcacca
      541 ggaactgcac cgcgaactcg gcatcgacca tgccgccggg gctgtgcttc acgtcgaagc
      601 ggtcgggctt caccggccgc gcgctgcgca gcttgttgcg catcgcgatg atctcggcct
      661 tcagcgcctc gcggtcgcgc ggcgccacga gcacggcctc gcgcacctgg tcgaatcgcg
      721 cgcccagctc ggcgccgtgg tcctcgctgc cgagcacgaa gcgggcgcgc gtcatggctt
      781 ggtgctccca ggtccacgcg gtgttgctgc cgcggccgag ctggtacttc tcgtaggcct
      841 cgaagctggt ggtcagcagg ccggagttgc cgttgggccg cagggcggtg tcgatctcga
      901 acaggtcgcc ctcgcgggtc ttcaccgtga gccagttgat gagcttgcgc acgtaggccg
      961 catagacctc gccggcgcgc tcgtcgtcgt cgtcgtagac gaacacgatg tcgaggtcgc
     1021 tgccgtagcc cagctcctta ccgccgagct tgccgtagcc gatgatcgcg aactgcggca
     1081 cctcgcgatg ccggttgcgc acgtgcggcc agcaccagcg ggcggtcacg cgcagcgtgg
     1141 cgtcggccag cgcgctgagg tcgtcggcca cctgctcgac ggtgagccgg ccgtccacgt
     1201 cgcgcgccag ggtgcggaac acctcggcgt ggtgcgcgtg gcgcagcagg ttcagcagcc
     1261 gctcctcgtc ggcctcgccg gtgcgggtga gcgatgcgtg gcgcgcgtcg agctcgcgct
     1321 cgaactcggc ggcgacgaag cggcccgaga gcatctcgtc gctcgcgagc tcgtcgatca
     1381 cgccggggtg ctgcatcagg tagcgtgcgg gccacttggc cgcgcccagc aggcgcagca
     1441 ggcgttcctg cacggcgggg cgctcgacca ggagcgccag gtagctctcg cggcgcatca
     1501 gcggctcgat ccagtcggcc cagcgcatgg cggcgtcgac gtggcgcggg gtctggcccg
     1561 cgccgtcgcc gtcgggctcc ttgagccact ggccggtgcg ctgcaccagc tggcgcaggc
     1621 gcgcgcgggt ttcctcgcgc agggccagca cgcggggctg ctcgcaccag tcgcggatgc
     1681 gctcggcgaa cacggggggc aggtcgccga gcaggtcggc caggtcggcg ggggcggcgg
     1741 ccttcttgcc gctgcacttg ccgttgcagg gtttctcgcc gccgagcagc ttgtcgaact
     1801 cctgcgcgac gaactcgcgg tgcgtgtcga gctgctcgag gaaggggcag cagctcgcgt
     1861 agcccatgct ctgggcgatc cagcgcaggt cgtcgtcgga taccggcagc acgtgggtct
     1921 gctggtcgtc gaggtactgg atgcggtgct cgacgcggcg caggaactcg taggccgcgg
     1981 ccagcgcgtc ggccgtctcc tgcggcatga ggttggcgcg cgccaggcgc tgcagcgcgt
     2041 cgagcgtggg ccgcgtgcgc agctcgggga actgcccgcc gcgcaccacc tgcagcagct
     2101 gcacggtgaa ctcgatctcg cggatgccgc cgcgcgagag cttcacgtcg ttggcacgct
     2161 ctggccggcc ggcgctgcgg cgcgccgact gctcgcggat ctgccggtgc agcacgcgca
     2221 gcgaatcgaa cacgctgtag tcgagatagc ggcggaacac gaagggcagc accgcgccgc
     2281 gcagcgcctg ggcctggcct gcgagcacga tgtcgcgcgg cgcgaccacg cggctcttca
     2341 tccaggcgaa gcgctcccac tcgcggccct gcacctggaa atactcttcc agcgc
//
LOCUS       DATCRO010000326         7520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_453435, whole genome shotgun sequence.
ACCESSION   DATCRO010000326 DATCRO010000000
VERSION     DATCRO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453435"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1741)
                     /locus_tag="VIN75_08850"
     CDS             complement(101..1741)
                     /locus_tag="VIN75_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8974313.1"
                     /translation="MTDTPTLFGTMDTCTIPNEDVVARPRPAGELPEPPIRHVAVVGG
                     GTAGWMTALLLANTKFGERLKVTVLESPQVGIIGVGEGSTPWLRKFFEALDIEEREWM
                     PACNATYKSGITFEGWSTRPGYESYFHPFASILDNLTMPQFVHNVHARLNDADVQAHP
                     DRFFISNKVAQSGRAPRPRRNFPFDIWYGYHFDSTLLGAFLAKKAAERGITHVARHMT
                     SVSLDERGDIRALELEGGDTLTADFFVDCTGFASLLIGKALQTPYVSFKENLFNDAAI
                     AMPTPSDGGPLRSQTISSAMRHGWAWKIPLTSRNGNGYVYSSAHVSADEAETELRTRL
                     GLLDADVPARHLKMRIGRYTRHWNHNCVAVGLSQGFIEPLEATALLFVQRTAGWLVEA
                     LEKGELDEPARAHFNDKVNAQFEGTRDYIVTHYKTNTRTDTPYWRDNATNTALSDPLR
                     LLLGAWLASKPIVGAIRQGQVGRGYPTLSWYCLLAGVGLFPEDADLRAPTAQEAQYDL
                     DGLDHLLERSVLNFPDHREFLEDIPAAVTEKTFQVYQW"
     gene            complement(1774..3360)
                     /locus_tag="VIN75_08855"
     CDS             complement(1774..3360)
                     /locus_tag="VIN75_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8974314.1"
                     /translation="MQSRRTEPPVQHVVVVGGGAAGWMTALLLSTSTFGARLKVTVLE
                     SPTAADLGVGEGSTPGLRGFFDSLDIDEAEWMPACNATYKTGVAFDGWSTRPGHENWF
                     HPFPSMLDTLTMPTFLRNVHARLDGADVHAHPDRFFVSRRVAIEGRAPRPVHEFPFEI
                     GYGYHLDPTLLGAFLRAKALQRGVAHVARPMRSATLDERGDIRALELDDGDSLAADFF
                     VDCSGSASLLIGEALKTPWVSFADGLVNDAAITFSTPHGDGPLMSQTVATALGHGWAW
                     KIPLATHGGSGYVYSSAHLSADAAETELRAHLGLLDADVPMRHLKMRMGRHAQHWNRN
                     CVAIGAAQGFIDPLEATALQSAQRAAASLVDVLERGDVGAGARASFNDGLNARADGER
                     DFVVTHYKSNSRVDTDYWRANAANDALSEPLQRLLHTWLSAQPIEPGIENGSFGIGHA
                     TLSWYCLLAGVGVFPEVRQTAANRAGADLSGIDDLVRRSTSNFPDHRAWLRDMPPHAE
                     RITLRRQPADAGRRATLGAL"
     gene            complement(3416..4423)
                     /locus_tag="VIN75_08860"
     CDS             complement(3416..4423)
                     /locus_tag="VIN75_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY8974315.1"
                     /translation="MTASLPSMPEVHGVDRARFEAEILPGNAPVVLRGVASGWPAVAK
                     GHASPLALAQYLATKDNGRPVDALMTRPEEGGRLFFDATMTGFNYVHSKLPVSHVLEQ
                     ILRYAQFERAPAVVVQSAAIVNCMPELKRDHVLPLLDDSVEPRAWIGTATVTPAHFDE
                     SHNIAVCVAGRRRFTLLPPEQVANLYVGPLDHAPAGRPISVVDFLKPDFDRFPRFRDA
                     LAHAQVAELEPGDAIYMPPLWWHHVESLAQLNMLVNYWWEPPPSPGLATLIYATAALR
                     ALPPAQRHAWRHLFEHYVFNAEPGDADHIPPDRRSVLGDMSPEDHVRLRKFLSERLAR
                     G"
     gene            complement(4556..7393)
                     /locus_tag="VIN75_08865"
     CDS             complement(4556..7393)
                     /locus_tag="VIN75_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8974316.1"
                     /translation="MKTKLTPIAAAAALALAGAVAPAFAQTADAPAPAASAAVAAAPA
                     PAAAKDDVQRVEVTGIRASLQSAINQKRIAETHVDVISAEDIGKMPDKNVADSLARVP
                     GVTILNSPTGGSGGFDERDRVGLRGTNPSLTQTLLDGHAVANGDWFVLDQTGAGVGRS
                     VSFSLLPSELVSRVEVHKNSVASDIEGGTAGSVNIVTRKPLEFSKQFTLEGSLGAVYA
                     DLPKKTDPQASVLVGWHNESKTFGFLVQGFDEKRHLRRDGVETFALDGTKAYPPVDAT
                     TNATLVAAGVPAGTKFVNLMGEALFTQDRNRKGGSISVELKPTSTMDFTLSGFSSKMD
                     ANNYNRNFMLVPFWSLDDSRHAGNLPTISNVNVQNGILTSVDYTFAAPTVVGVYDLIS
                     RKASASTNYLNLDGSWNVTSSLKLSGNGGVSNGEGRTGSQDVWEGDVYGTGGSYQLHG
                     SNNAPSFGMTLAPVPAGKVGTGPTGSFATDWIFGDQDVDVKDKDTWGQIDAEYSLDSG
                     MLRSLKFGLRGNEHKRYTDGPIIAQGPNWGTDPFANPIPASDFTQSYPSNFGSGLGGS
                     FPSGVMFPTPAALAAYDNTYAARPISRRIVTHEYSVKEQNQAAYVQGNLEGEAWSGNV
                     GVRVVNTKETSLIWAPTSDTSLPGYVDSVWAAGAIPYTGYYQTYDKRSYTDVLPSASI
                     RIDVTPKVVARFALSKTMTRPDYTALSSAQLLGNYSDPTGKSVGSGSTGNPDLNPVRS
                     TNVDANLEYYFAPRAFVSVGAFHMEMSSYITDGIKTGTYTTDLVGGTPGQTVTAPFAL
                     STSVNMKASVTGMEFAVETPIFENFGVSANYTLADAHDDTGHVLKGAVKNSGTVSGYY
                     ENDMFSARINYNYSGDNYLGRDRGTDYFQRGVGVVSASLGLKFGQHLSFSLDAQNLND
                     PILKYYGDTKDEPRAIYRNGRQFYLSAHFKY"
BASE COUNT         1125 a         2401 c         2737 g         1257 t
ORIGIN      
        1 gatcgggccg ggccttgggc gcgaggcggc cggagcgccg gacgccgatc gcgacggccc
       61 gacgaagccc cggcgccgat cgcgcgccgc cgcgctcgac tcaccactga tagacctgga
      121 atgtcttctc cgtcacggcc gccgggatgt cttccaggaa ttcgcgatgg tcggggaagt
      181 tgagcacgct gcgttcgagc agatggtcga gcccgtccag gtcgtactgc gcttcctgcg
      241 cagtcggcgc gcgcaggtcg gcgtcctccg ggaacaggcc gacgcccgcc agcaggcagt
      301 accacgacag cgtcggatag cccctgccga cttggccctg gcggatggcg cccacgatcg
      361 gcttgcttgc gagccacgcg cccagcagca ggcgcaacgg gtcggacagc gcggtgttgg
      421 tggcgttgtc gcgccagtac ggcgtatcgg tgcgggtatt cgtcttgtag tgggtgacga
      481 tgtagtcgcg cgtgccctcg aactgcgcgt tgaccttgtc gttgaagtgc gcgcgcgccg
      541 gttcgtcgag ctcgcccttc tcgagcgcct cgacgagcca ccccgccgtg cgctgcacga
      601 acagaagtgc cgtggcctcc aacggctcga tgaagccctg agacaggccg acggccacgc
      661 agttgtggtt ccagtgccgc gtgtagcggc cgatgcgcat cttcaggtgg cgcgcgggga
      721 cgtcggcgtc gagcagcccc aggcgcgtgc gcagctcggt ctcggcctcg tcggccgaga
      781 cgtgcgcgct gctgtagacg tagccgttgc cgttgcgcga ggtcagcggg atcttccacg
      841 cccagccgtg gcgcatcgcg ctggaaatgg tctgcgacct cagcggcccg ccgtccgacg
      901 gcgtgggcat cgcaatggcc gcgtcgttga acaggttctc cttgaacgag acgtacggcg
      961 tctgcagcgc cttgccgatc agcagcgagg cgaagcccgt gcagtcgacg aagaagtcgg
     1021 cggtcagcgt atcgccgccc tcgagctcga gggcgcggat gtcgccgcgc tcgtcgagcg
     1081 agacggacgt catgtggcgc gccacgtgcg tgatcccgcg ctcggcggcc ttcttggcga
     1141 ggaatgcgcc gagcagcgtg gagtcgaagt ggtagccgta ccagatgtcg aacgggaaat
     1201 tgcgccgcgg ccggggcgca cggcccgact gcgcgacctt gttggagatg aagaaccggt
     1261 cgggatgggc ctggacatcg gcgtcgttca ggcgcgcgtg cacgttgtgc acgaactgcg
     1321 gcatcgtgag gttgtccagg atcgatgcga acgggtggaa gtagctctcg tagccggggc
     1381 gagtggacca gccctcgaac gtgatgccgc tcttgtacgt ggcgttgcac gcgggcatcc
     1441 attcgcgctc ctcgatgtcc agcgcctcga agaacttgcg cagccacggc gttgagcctt
     1501 cgccgacgcc gatgatgccc acctgcggcg actccagcac cgtcaccttg aggcgctcgc
     1561 cgaacttcgt gttggccagc agcagcgcgg tcatccagcc ggcggtgccg ccacccacca
     1621 ccgccacgtg gcggatcggc ggctcgggca gttcgcccgc gggacggggg cgcgcgacga
     1681 cgtcctcgtt ggggatcgtg caggtgtcca tcgtgccgaa gagcgtgggg gtgtccgtca
     1741 tgccgctgcc agtgtcaggg gtcgggtgga gggtcagagc gcgccgagcg tggcgcggcg
     1801 ccctgcgtcc gcgggctggc gccggagggt gatgcgttcc gcgtgtggcg gcatgtcgcg
     1861 cagccaggcc cggtggtcgg gaaagttgga ggtactgcgc cgcacgaggt cgtcgatgcc
     1921 cgagaggtcg gcgcccgccc tgttcgcggc cgtctgccgc acctcgggga acacacccac
     1981 tccggcgagc aggcagtacc acgacaacgt ggcatggccg atgccgaagc tgccgttctc
     2041 gatgccgggc tcgatgggct gggccgagag ccacgtgtga agcagccgtt gcaggggctc
     2101 cgacaacgcg tcgttggccg cgttcgcacg ccagtagtcg gtgtcgacgc gcgagttgct
     2161 cttgtagtgg gtgacgacga agtcgcgctc gccgtccgcg cgggcattga ggccgtcgtt
     2221 gaacgaggcc cgcgcgcccg cgccgacgtc gccgcgctcg agcacgtcca ccaatgacgc
     2281 cgcggcgcgc tgtgcggact ggagcgcggt ggcttccagc ggatcgatga agccctgcgc
     2341 cgcgccgatg gccacgcagt tgcggttcca gtgctgggcg tgccggccca tgcgcatctt
     2401 caggtggcgc atgggcacgt cggcgtcgag caggcccagg tgtgcgcgca gctcggtctc
     2461 ggcagcgtcg gcggacaagt gcgcgctgct gtagacgtag ccgctgccgc cgtgcgtggc
     2521 cagcgggatc ttccacgccc agccatgccc gagggccgtc gccacggtct gcgacatcag
     2581 cggcccgtcg ccatggggcg tggaaaacgt gatggccgcg tcgttgacca ggccgtcggc
     2641 gaatgacacc cagggcgtct tcaacgcctc gccgatcagc agcgacgcgg aacccgagca
     2701 gtcgacgaag aagtcggccg cgaggctgtc gccatcatcg agttcgagcg cgcggatgtc
     2761 gccgcgctcg tcgagcgtgg ccgaccgcat gggccgcgcg acgtgcgcga cgccgcgctg
     2821 caacgccttg gcgcgcaaga acgcgcccag cagggtgggg tcgaggtggt agccgtagcc
     2881 gatctcgaac gggaactcgt gcacgggccg cggcgcgcgc ccttcgatcg ccacgcgccg
     2941 cgagacgaag aagcggtcgg gatgggcgtg gacgtccgcg ccgtccagcc gggcgtgcac
     3001 gttgcgcagg aatgtcggca tcgtgagcgt gtccaacatc gacgggaacg ggtggaacca
     3061 gttctcgtgg cccgggcgcg tgctccagcc gtcgaacgcg acaccggtct tgtacgtcgc
     3121 gttgcaggca ggcatccact ccgcttcgtc gatgtccagg ctgtcgaaga agccgcgcaa
     3181 cccgggcgtg gagccctcgc cgacgcccag gtcggccgcc gtcggcgact cgagcaccgt
     3241 caccttcagg cgcgcgccga acgtcgacgt ggacaacaac aacgcggtca tccagcccgc
     3301 cgcgccgccg cccacgacga cgacgtgctg gacgggtggc tcggtgcgac ggctctgcat
     3361 gtgagggagt ggcaggttat gggtcatgga acgccgacgg ccaggcagcg cgcgctcatc
     3421 cgcgggcgag ccgctcgctc aggaacttgc gcagtcgcac gtgatcctcg ggactcatgt
     3481 cgcccagcac gctgcgccgg tcgggtggaa tgtgatcggc gtcgcccggc tcggcattga
     3541 acacgtagtg ctcgaagagg tggcgccagg catgtcgctg cgccggcggc agggcgcgca
     3601 gcgcggcggt cgcgtagatc agggtggcca ggccaggcga cggcggcggc tcccaccagt
     3661 agttcacgag catgttgagc tgcgccagcg attccacgtg gtgccaccac agcggcggca
     3721 tgtagatggc gtcgccgggc tccagctcgg cgacctgcgc gtgggccaac gcgtcgcgga
     3781 agcgcggaaa gcgatcgaaa tcgggcttga ggaaatccac gacggagatc ggccggccgg
     3841 cgggcgcgtg atcgagcggt ccgacgtaaa ggttggcgac ctgctccggc ggcagcagcg
     3901 tgaagcgcct gcgtccggcc acgcacacgg cgatgttgtg cgactcgtcg aagtgcgccg
     3961 gcgtgacggt cgcggtaccg atccaggcac gcggctccac gctgtcgtcc agcagcggca
     4021 gcacgtggtc gcgcttgagc tccggcatgc agttgacgat cgccgcgctc tggaccacca
     4081 ccgccggcgc ccgctcgaac tgagcgtaac gcaggatctg ctcgagcacg tgcgacaccg
     4141 gcaacttgct gtgcacgtaa ttgaagcccg tcatcgtggc gtcgaagaag aggcggccgc
     4201 cctcctcggg gcgcgtcatg agcgcgtcca cgggccggcc gttgtccttg gtggcgagat
     4261 attgcgccag cgccagcggc gaagcgtggc ccttggccac cgcgggccag ccgctcgcca
     4321 ccccacgcag gacgacgggt gcgttgccag gcaggatctc ggcctcgaac ctggcgcggt
     4381 cgacgccatg cacctcgggc atggagggaa gggacgcggt catcgtggca tcgctgaacg
     4441 tggcgtgatc ttcgagcata gccgagcccg gcatcgggtc gagatggaaa aaaggacggc
     4501 gcgggcgccg tcctttgcgt tgtcgcgaag acccgccgaa gcgggccgca ggcgatcagt
     4561 acttgaagtg ggcgctcagg tagaactggc ggccgttgcg atagatcgcg cgcggctcgt
     4621 ccttggtgtc gccgtagtac ttcaggatcg ggtcgttcag gttctgggcg tccaggctga
     4681 aggacaggtg ctggccgaac ttcaggccca gcgaggcgga caccacgccg acaccacgct
     4741 ggaagtagtc ggtgccacgg tcgcggccca ggtagttgtc gccgctgtag ttgtagttga
     4801 tgcgggccga gaacatgtcg ttctcgtagt agccgctcac cgttcccgag ttcttgaccg
     4861 cgcccttgag cacgtggccc gtgtcgtcat gcgcatcggc cagcgtgtag ttcgcgctga
     4921 cgccgaagtt ctcgaagatc ggcgtctcga ccgcgaactc catgcccgtg acggaggcct
     4981 tcatgttgac cgacgtgctc agggcgaacg gggccgtgac ggtctggccc ggcgtgccgc
     5041 ccacgagatc ggtcgtgtag gtccccgtct tgatgccgtc ggtgatgtag ctggacatct
     5101 ccatgtggaa ggcacccacc gacacgaagg cgcgcggcgc gaagtagtac tcgaggttgg
     5161 cgtcgacgtt ggtcgagcgc accggattga gatcggggtt gccggtgctg ccggagccca
     5221 cggacttgcc ggtcgggtcg gagtagttgc ccagcagctg ggccgacgac agcgccgtgt
     5281 agtcaggacg ggtcatcgtc ttggacagcg cgaagcgggc cacgaccttc ggcgtcacgt
     5341 cgatgcggat gctggcgctg ggcagcacgt ccgtgtagct gcgcttgtcg taggtctggt
     5401 agtagccggt gtagggaatc gcgccggcgg cccaaacgga gtcgacgtag cctggcaggg
     5461 aggtgtcgct ggtgggcgcc cagatcagcg aggtttcctt cgtgttcacg acgcgcacgc
     5521 cgacattgcc gctccaggct tcgccttcga ggttgccctg cacgtaggcc gcctggttct
     5581 gctccttcac ggagtattcg tgcgtcacga tgcggcgcga aatcgggcgc gcggcatagg
     5641 tgttgtcgta cgccgccagc gcggccggcg tcgggaacat cacgcccgag gggaagctgc
     5701 cacccaggcc gctgccgaag ttgctcggat agctctgggt gaagtcggag gcggggatcg
     5761 gattggcgaa cggatcggtc ccccagttcg ggccctgggc gatgatcggg ccgtcggtgt
     5821 agcgcttgtg ctcgttgccg cgcaggccga acttcaggct gcgcagcatg ccgctgtcga
     5881 gcgagtactc ggcatcgatc tggccccagg tgtccttgtc cttcacgtcc acgtcctggt
     5941 cgccgaagat ccagtcggtg gcgaaggagc cggtcgggcc ggtgccgacc ttgcctgcag
     6001 gcacgggggc cagcgtcatg ccgaacgagg gcgcgttgtt gctgccgtgg agctggtagc
     6061 tgccgccggt gccatagacg tcgccttccc agacgtcctg cgagcccgtg cggccttcgc
     6121 cgttggacac gccgccgttg cccgacagct tcaggctgct ggtgacgttc caggaaccgt
     6181 cgaggttcag atagttggtc gaagccgacg ccttgcgcga gatcaggtca tacacgccca
     6241 cgaccgtcgg ggccgcgaag gtgtagtcga ccgaggtcag gatgccgttc tggacgttga
     6301 cgttgctgat ggtgggcagg ttgcccgcgt gccgcgagtc gtcgagcgac cagaagggca
     6361 ccagcatgaa gttgcggttg tagttgttcg cgtccatctt ggacgagaag cccgacagcg
     6421 tgaagtccat cgtgctggtc ggcttgagtt cgacgctgat ggacccgccc ttgcggttgc
     6481 gatcctgcgt gaacagggcc tcgcccatca ggttgacgaa cttggtgcct gcgggaaccc
     6541 ctgcggccac gagcgtcgcg ttcgtggtgg catcgacggg cggatacgcc ttggtgccgt
     6601 cgagagcgaa cgtctcgacg ccgtcgcggc gcaggtggcg cttttcgtcg aagccttgca
     6661 ccaggaagcc gaacgtcttg gactcgttgt gccagcccac cagcacgctg gcctgcggat
     6721 cggtcttctt cggcaggtcg gcgtacacgg cgccgaggct gccttccagg gtgaactgct
     6781 tggagaattc gagcggcttg cgggtgacga tgttcaccga accggccgtg ccgccttcga
     6841 tgtcggaagc gacggagttc ttgtgcacct cgacgcggct cacgagctcg gacggcagca
     6901 gcgagaagct gacgctgcgg cccacgcccg cgccggtctg atccagcacg aaccagtcgc
     6961 cattggcgac cgcgtggccg tccagcagcg tctgcgtcag gctcgggttg gtgccccgca
     7021 ggcccacgcg gtcgcgttcg tcgaagccgc cggagccacc cgtggggctg ttcaggatgg
     7081 tcacgcccgg aacgcgcgcc agcgagtcgg ccacgttctt gtcgggcatc ttgccgatgt
     7141 cttcggcgga gatgacgtcg acgtgcgtct cggcgatgcg cttctggttg atggcggatt
     7201 gcagcgaggc gcggatgccc gtgacttcca ctcgttggac atcgtccttg gccgcggcgg
     7261 gcgccggggc ggcggccacg gcggcggagg ccgcgggggc cggcgcatcg gcggtctgcg
     7321 cgaatgccgg ggcgaccgcg ccagccagcg cgagcgcggc agcggcggca atgggcgtaa
     7381 gtttcgtctt catggggctc ctcttttcgg gtggcagcat ttcttcggga acgcggccgg
     7441 ttgggttttg ggtcgtgccg tctctccacg agctcagacg gtgcaccctc gggctagaaa
     7501 cctggtatgc gacaaggtag
//
LOCUS       DATCRO010000327         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_363398, whole genome shotgun sequence.
ACCESSION   DATCRO010000327 DATCRO010000000
VERSION     DATCRO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363398"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2505
                     /locus_tag="VIN75_08870"
     CDS             <1..2505
                     /locus_tag="VIN75_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8974317.1"
                     /translation="IAQATVPQDRLFQVLDDSMRKAGSYSHRYRLFTADGGLRRVQAH
                     WEVIAGADGQPEQVVGIVMDDTETFKLASSMDDAAAQLQLATELAEVSMWRHDLVTQR
                     VYMNGRAAVVMGMEARPEGIPIDEIRARVHPDDLLEVLSTYQRALTSNRAEDAQARYL
                     GQDGRWHHIFTRRVTQRDGFGAPVALNGVALDITEQMERVQRVDELARRLDDVTSASG
                     IGVWRVLRDDDSAEWNAQMRAMTGLPADEAAPGFMAWLERFVHPDDRERVRDVGLEWI
                     KQPTAQLELAHRIVNVHGQVRHVVSRTRAEPRGSPYRATGVVIDVTERELALEALREA
                     NERSAMATRAAGIGIWEWDADAGITRWDEEMFVLRGREPRPAPPDFDEMSSWVHPDDR
                     AYVNDQIQTARVGDGQASYEFRILLPDGRVRWIASRSLPVRDERGRTRMRLGVNWDIT
                     EAHEAAAARHERELVLRESQAKSELLARMSHELRTPMNAVLGFTRLLMADDAQGDDAE
                     TTRQARLEHIHSAGEHLLTLINNVLELSRLDGPARDVVLQPVRVATVIEQTLPLVEGL
                     AVQHGVTVVAPPGALHVRADATRLKQVLVNLLTNAIKYNRPGGSVRVDVSPDGGQVLI
                     RVSDDGMGMTSEQQQHAFEPFNRAGAERAGIEGTGIGLTIVKALVEGMGGRIDVHSTR
                     GVGSTFEVRLDAAAESGPLALPARRLLYIEDNAVNVMIVAELVRRRPGIEFDSMPDGA
                     SGVARAAQTLPGLVLVDMQLPDFDGLEVLRRLRADPATANLRVVALSANAIPADIQRA
                     LQAGFDDYWTKPLDFKVFNAALDQVFGGGATAPRPS"
BASE COUNT          400 a          976 c          966 g          328 t
ORIGIN      
        1 atcgcgcagg ccacggtgcc gcaggaccgc ctgttccagg tgctggacga ctcgatgagg
       61 aaggcgggca gctactcgca ccgctaccgc ctgttcaccg ccgacggcgg cctgcggcgc
      121 gtgcaggcgc actgggaggt gatcgccggc gccgacggcc agcccgagca ggtggtgggc
      181 atcgtgatgg acgacaccga gacgttcaag ctcgcctcgt cgatggacga cgccgccgcc
      241 cagctgcaat tggccaccga gctggccgag gtgtcgatgt ggcggcacga cctcgtcacg
      301 cagcgcgtgt acatgaacgg ccgcgccgcg gtcgtcatgg gcatggaggc gcgccccgag
      361 ggcatcccga tcgacgagat ccgcgcccgc gtccaccccg acgacctgct tgaggtgctt
      421 tcgacctacc agcgggcgct cacgtccaac cgcgccgagg acgcgcaggc gcgctacctc
      481 ggccaggacg gccgctggca ccacatcttc acgcgccgcg tcacgcagcg cgacggcttc
      541 ggtgcgcccg tggccctgaa cggcgtggcg ctcgacatca ccgagcagat ggagcgcgtg
      601 cagcgcgtcg acgagctcgc gcgccggctc gacgacgtga catcggcctc gggcatcggc
      661 gtctggcgcg tgctgcgcga cgacgactcg gccgaatgga acgcgcagat gcgcgcgatg
      721 acgggcctgc ctgccgacga agccgcgccc ggcttcatgg cctggctcga acgcttcgtg
      781 cacccggacg accgcgagcg cgtgcgcgac gtcggcctcg aatggatcaa gcagccgacg
      841 gcgcagctgg aactcgcgca ccgcatcgtc aacgtgcacg ggcaggtgcg ccacgtcgtg
      901 tcgcgcacgc gggccgagcc gcgcggcagc ccctaccgcg ccaccggcgt ggtgatcgac
      961 gtcaccgagc gcgagctcgc gctggaggcg ctgcgcgagg ccaacgagcg cagcgcgatg
     1021 gccacccgcg cggccggcat cggcatctgg gaatgggacg ccgacgccgg catcacgcgc
     1081 tgggacgagg agatgttcgt gctgcgtgga cgcgagccgc ggcccgcgcc ccccgacttc
     1141 gacgagatga gctcctgggt gcacccggac gaccgcgcct acgtcaacga ccagatccag
     1201 accgcgcgcg tcggcgacgg ccaggcgtcg tacgagttcc gcatcctgct gcccgacggg
     1261 cgggtgcgct ggatcgcctc gcgctcgctg ccggtgcgcg acgagcgcgg ccgcacccgc
     1321 atgcggctcg gcgtgaactg ggacatcacc gaggcgcacg aggcggccgc cgcgcgccac
     1381 gagcgcgagc tggtgctgcg cgagagccag gccaagtccg agctgctcgc gcgcatgagc
     1441 cacgagctgc gcacgccgat gaacgccgtg ctgggcttca cccgcctgct gatggccgac
     1501 gacgcacagg gcgacgacgc cgagacgacg cgccaggcgc gcctggaaca catccactcg
     1561 gccggcgaac acctgctcac gctgatcaac aacgtgctgg agctgtcgcg cctggacggc
     1621 cccgcgcgcg acgtcgtgct gcagccggtg cgggtggcca ccgtcatcga gcagacgctg
     1681 ccgctggtcg agggactggc cgtgcagcac ggcgtgacgg tcgtcgcccc gcccggcgcg
     1741 ctgcacgtgc gcgccgacgc cacgcgcctg aagcaggtgc tggtcaacct gctgacaaac
     1801 gcgatcaagt acaaccgtcc cggcggcagc gtgcgcgtcg acgtctcgcc cgacggcggg
     1861 caggtgctga tccgcgtgtc ggacgacggc atgggcatga cgtccgagca gcagcagcac
     1921 gcgttcgagc ccttcaaccg cgccggggcc gaacgcgcgg gcatcgaggg caccggcatc
     1981 ggcctgacca tcgtcaaggc gctcgtcgag ggcatgggcg ggcgcatcga cgtgcacagc
     2041 acgcgcggcg tcggcagcac gttcgaggtg cggctcgacg ccgcggccga gtccggcccg
     2101 ctggcgctgc cggcgcggcg cctgctgtac atcgaggaca acgcggtcaa cgtgatgatc
     2161 gtcgccgagc tggtgcgccg ccgccccggc atcgagttcg acagcatgcc cgacggcgcc
     2221 agcggcgtcg cgcgcgcggc gcagacgctg cccggcctcg tgctggtgga catgcagctg
     2281 cccgacttcg acggcctgga agtgctgcgc cggctgcgcg ccgaccctgc caccgccaac
     2341 ctgcgcgtgg tggcgctgtc ggccaacgcg atccccgccg acatccagcg cgcgctgcag
     2401 gccggcttcg acgactactg gaccaagccg ctggacttca aggtgttcaa cgccgcgctc
     2461 gaccaggtgt tcggcggcgg cgcgacggcg ccgcgcccgt catgagcccg cacggccgcc
     2521 ggacgggcga atcgaccgca gcgcgcagcg cggaggttgc ctgatgggcg cgctgcgctg
     2581 gctcacgctc gacgtcagcg acaacgacga gggcgtggtc acgatcgagg cgctcgcgtc
     2641 cacggacgcc gcgggccacg cgttcgcgct
//
LOCUS       DATCRO010000328         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_115979, whole genome shotgun sequence.
ACCESSION   DATCRO010000328 DATCRO010000000
VERSION     DATCRO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115979"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VIN75_08875"
     CDS             complement(<1..398)
                     /locus_tag="VIN75_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974318.1"
                     /translation="MRIVSSLTAALLAGLSAASAHAAPSLLFIGNSFTYGQGSAVHYY
                     RASTVTDLNGEGVGGLPALFKSFATQAGLDYDVYLETHGGVGLDWHLADKSGVIGSRP
                     WDGVVMHGLSTLDKDKPGDPALLVDTVRRMS"
     gene            complement(496..1947)
                     /locus_tag="VIN75_08880"
     CDS             complement(496..1947)
                     /locus_tag="VIN75_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8974319.1"
                     /translation="MDAPDTLYLQIAETLAASIRGGVLARGERLPSLRDLAAQHGVSL
                     STAVQAYRALEDMRLVETRPRSGFFVAARPPRPPVPETTQPPADSREVDIDAQAAHAM
                     RYAHEEGYITFGAAAPSDELFSVERIRRTVMRAAQRHRAVLCEYVLGSGNETLRRAIA
                     RYALRLGCQLRPRDIVATNSCLESITLCLRAVAKPGDIVALESPTYFGFLEILQSLHL
                     RALEIPTHPRDGLSLDALELALDTQPVKALLLSPTLSNPMGGSMPTAERKRLARLVAS
                     RGIAMIEDVLHNDLAEQEDKRRAVRSFDTSGHVMLCGSFSKTVAPGLRVGWVEAGRWA
                     EPVRRMKMATSGSQTTFIELAMADLLNHTGNEAGFRQMRHTIGVRVDEARRLIAGSFP
                     KGTRVTDPAGGYILWVEMPRGVDSQALFTACLAERIAIAPGTLFSASDRYRHCIRLGV
                     GGRWDDTQRHALMRIGELARALLAAAPALPEAA"
BASE COUNT          306 a          797 c          817 g          302 t
ORIGIN      
        1 gacatgcgcc gcacggtgtc gaccagcagc gcggggtcgc cgggcttgtc cttgtcgagc
       61 gtgctcagcc cgtgcatgac gacgccgtcc cacggccgcg agccgatgac gccggacttg
      121 tccgccaggt gccagtccag cccgacgccg ccgtgcgtct cgaggtacac gtcgtagtcc
      181 agccccgctt gggtggcgaa ggacttgaac agcgcgggca gcccgcccac gccctcgccg
      241 ttgaggtcgg tgacggtgct cgcgcggtag tagtgcaccg cggagccttg gccgtaggtg
      301 aagctgttgc cgatgaacag caggctgggg gcggcgtggg ccgacgcggc ggacaggccg
      361 gcgagcaggg ccgcggtgag cgaagagacg atcctcaagt cggactccgt gtgtggcgcg
      421 acccaaccag ccgcggctcg tgacgacgga tcttagcgag cgggcaaggc cctgcgccat
      481 cgcaggtcgc ctgcgtcacg ccgcctcggg caacgccggc gccgccgcga gcagcgcgcg
      541 cgccagctcg ccgatgcgca tcagcgcgtg gcgctgcgtg tcgtcccagc gcccgcccac
      601 gcccagccgg atgcagtggc ggtagcggtc gctggcgctg aacagggtgc ccggcgcgat
      661 ggcgatgcgc tcggccaggc acgcggtgaa cagcgcctgc gagtcgacgc cgcgcggcat
      721 ctccacccac aggatgtagc cgccggccgg gtcggtgacg cgcgtgccct tgggaaagct
      781 gccggcgatc aggcgccgcg cctcgtcgac gcgcacgccg atcgtgtggc gcatctggcg
      841 gaagcccgcc tcgttgccgg tgtggttcag cagatcggcc atcgcgagct cgatgaacgt
      901 cgtctgcgag ccgctggtgg ccatcttcat gcggcgcacc ggctcggccc agcgccccgc
      961 ctcgacccag cccacgcgca ggcccggcgc gaccgtcttg gagaacgaac cgcacagcat
     1021 gacgtggccg ctggtgtcga acgagcgcac ggcgcggcgc ttgtcctcct gctcggcgag
     1081 gtcgttgtgc agcacgtcct cgatcatcgc gatgccgcgc gacgcgacca ggcgcgcgag
     1141 gcgcttgcgc tcggccgtcg gcatgctgcc gcccatcgga ttggacagcg tgggcgacag
     1201 cagcagcgcc ttcacgggct gcgtgtcgag cgccagctcc agggcgtcca gcgacaggcc
     1261 gtcgcgcgga tgcgtcggaa tctcgagcgc gcgcaggtgc aggctctgca ggatctcgag
     1321 gaagccgaag tacgtgggcg actcgagcgc gacgatgtcg cccggcttgg cgaccgcgcg
     1381 caggcacagc gtgatcgact ccaggcagct gttggtggcg acgatgtcgc gcggccgcag
     1441 ctggcagccc agccgcagcg cgtagcgcgc gatggcgcgg cgcagcgtct cgttgcccga
     1501 gcccagcacg tactcgcaca ggacggcgcg atggcgctgc gccgcgcgca tcaccgtgcg
     1561 gcggatccgc tccaccgaga acagctcgtc gctgggcgcc gcggcgccga aggtgatgta
     1621 gccctcctcg tgcgcgtagc gcatcgcgtg cgcggcctgg gcgtcgatgt cgacctcgcg
     1681 cgagtcggcg ggcggctgcg tcgtctcggg caccggcggc ctgggcgggc gcgccgcgac
     1741 gaagaagccg gaacgcgggc gcgtctccac gagccgcatg tcctccagcg cgcggtaggc
     1801 ctgcacggcg gtcgacagcg acaccccgtg ctgcgccgcg agatcgcgca gcgacggcag
     1861 ccgctcgccg cgggcgagca cgccgccgcg aatcgaggcc gcgagcgtct cggcgatctg
     1921 caggtagagc gtgtcgggcg cgtccatggc ccgattctgt ctgcaagccg gcaaggcaga
     1981 ccagtacaga tggcgggcaa ttcgaccgtg acagtggcgc cgctggcgga tctgtactgc
     2041 accagaaggc cgcgggttgt ggctgtctcg cgcgggcgcc cgctcctaga gtcgattcca
     2101 tggaacacgc cctcaccctg ccgccgtccc tcgcccgtcg cccggccgag gcccccgacc
     2161 acgtcctgcg cctgtcttcc cgggccacgt tcgcgcgcca gctgccggcg ggcgcgcgca
     2221 tc
//
LOCUS       DATCRO010000329         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_948310, whole genome shotgun sequence.
ACCESSION   DATCRO010000329 DATCRO010000000
VERSION     DATCRO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948310"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VIN75_08885"
     CDS             <1..360
                     /locus_tag="VIN75_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEY8974320.1"
                     /translation="PERASARMPELPHFTRLACLPRDAFYEYGERIPLLDDQGQPNKA
                     LDGRVCCDQITPYPPGIPVLVPGQVITSEIIAFLTRIMRMQKSIEMHGLATHDGEPSL
                     RVLAPGELEAMAARSAL"
     gene            complement(357..896)
                     /locus_tag="VIN75_08890"
     CDS             complement(357..896)
                     /locus_tag="VIN75_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY8974321.1"
                     /translation="MSSADDVLAFWFDPATEPDWWRRSDAFDARCAARFGALHESATR
                     CELWQWRTTAEGRLAEIILLDQFSRNLHRDSPRAFAADPLALGLAQEAVALGAHRALD
                     PRRRVFMLMPYMHSESRAVHEVALQVFADCPAGNLDYERQHKAIVDRFGRYPHRNAVL
                     GRASTPEEIEFLKTPGSSF"
     gene            995..2056
                     /locus_tag="VIN75_08895"
     CDS             995..2056
                     /locus_tag="VIN75_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8974322.1"
                     /translation="MNQPPVRRKRSPSTAFTPSGSGTVTLGMVAKAAGVSPSTVSRIL
                     NGTATVSPEKREAIDEAIRTLGFRPNPVARGLAGGRTLSVGVLTQTINSPFYGEALRG
                     IEDRLEVAGYIPIFVSGHWHEAEEHKAMDALMSRRVDGLIVLAGRMSNDALETYSNQV
                     PMVVVGRELRTDRIFSIDFDNFTGGLLATQHLIECGHHRIAFIAGDPLHKDALDREAG
                     YRAALERAGIPFDPALVLMGDFTEAGGLLAVNRLLESGLSFSAIFAANDQTAIGAALG
                     LYRRNVRVPDDVSLVGFDDLAPAKFSVPPLTTIRQSVYEIGSEAAAAMLAMLKGEEPG
                     ADLPPPELVARESTRRVLR"
     gene            2645..3661
                     /locus_tag="VIN75_08900"
     CDS             2645..3661
                     /locus_tag="VIN75_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8974323.1"
                     /translation="MTSSDDSQRGPRTFATRRRSVLAAGLAAAIAPAVRATEQRTLTV
                     AAFPLVDQIVKDAIPQWQKRHPDVAIDIVMRQYDDHHTAMTTALSTAVYLPDVMALEA
                     SYVGKFSDGGGLSDLLQPRYDIGRYRDRFVAYAYDQAINRKGQVVAAPTDIGPGTMLY
                     RQDLLERAGLVASDLQPSWDAYIDAGIRIKKATGANLIGNVRTFKDLVVRGGLKKGQG
                     IYFDSDSNVLVDTPRFHRAFELSLRMRRNGLEGHLETWSNEWAEAFRRNATATELGGS
                     WMVGQLANWVAPATKGLWRAAQLPESTWVSYGGTYYAIPRDSDPTRKALSDVARLLEL
                     RARR"
BASE COUNT          576 a         1347 c         1278 g          547 t
ORIGIN      
        1 cccgagcgcg cgagcgcccg catgcccgag ctgccgcact tcacgcgcct cgcctgcctg
       61 ccgcgcgacg cgttctacga gtacggcgag cgcatcccgc tgctggacga ccagggccag
      121 ccgaacaagg ccctcgacgg ccgcgtctgc tgcgaccaga tcacgccgta cccgccgggc
      181 atcccggtgc tggtgcccgg ccaggtgatc acgtccgaga tcatcgcctt cctcacgcgc
      241 atcatgcgca tgcagaagtc catcgagatg cacggcctgg ccacgcacga cggcgagccg
      301 tcgctgcgcg tcctggcgcc gggcgagctc gaggcgatgg cggcgcggtc cgcgctctag
      361 aacgacgacc ccggcgtctt caggaactcg atctcctcgg gcgtggacgc gcgcccgagc
      421 accgcgttgc gatgcggata gcggccgaag cggtcgacga tggccttgtg ctggcgctcg
      481 tagtcgaggt tgccggcggg gcagtcggcg aacacctgca gcgcgacctc gtgcaccgcg
      541 cgcgactcgc tgtgcatgta gggcatcagc atgaagacgc gccggcgcgg gtcgagcgcg
      601 cgatgggcgc cgagcgccac ggcctcctgc gccaggccca gtgccagcgg gtccgccgcg
      661 aacgcgcgcg gcgagtcgcg atgcaggttc cgcgagaact ggtcgagcag gatgatctcc
      721 gcgagccggc cttcggcggt ggtgcgccat tgccagagct cgcagcgcgt cgccgattca
      781 tgcagcgcgc cgaagcgtgc ggcgcagcgc gcgtcgaacg cgtcggagcg acgccaccag
      841 tcgggctcgg tggcggggtc gaaccagaag gcgaggacat cgtcggcaga ggacatgcgg
      901 ccatgatacg agcccgcatc gggcctgggg atgcgcgcga aagcgctacc gcgcctacac
      961 tccggggatt gctcagccgt caggcccttc tccgatgaac caaccgcccg tccgtcgcaa
     1021 gcgctctccc agcaccgcgt tcaccccgtc cggctcgggc acggtgaccc tcggaatggt
     1081 ggccaaggcg gccggggtct cgcccagcac ggtctcgcgc atcctcaatg gcacggccac
     1141 ggtcagcccc gagaagcgcg aggcgatcga cgaggcgatc cgcaccctcg gcttccgccc
     1201 gaacccggtc gcgcgcggcc tggccggcgg ccgcacgctg agcgtgggcg tgctgacgca
     1261 gaccatcaac agcccgttct acggcgaagc gctgcgcggc atcgaggacc gcctcgaggt
     1321 ggccggctac atcccgatct tcgtcagcgg ccactggcac gaggccgagg agcacaaggc
     1381 gatggacgcg ctgatgtcgc gccgcgtcga cgggctgatc gtgctggcgg ggcgcatgtc
     1441 caacgacgcg ctcgagacct actcgaacca ggtgccgatg gtggtggtgg ggcgcgaact
     1501 gcgcaccgac cgcatcttct cgatcgactt cgacaacttc accggcggcc tgctcgccac
     1561 gcagcacctg atcgaatgcg gccaccaccg catcgcgttc atcgccggcg atccgctgca
     1621 caaggacgcg ctcgatcgcg aggccggcta ccgcgccgcg ctggaacgcg cgggcatccc
     1681 gttcgatccg gcactggtgc tgatgggcga cttcacggag gccggcggcc tgctggccgt
     1741 caaccggctg ctggagagcg gcctgtcgtt cagcgcgatc ttcgccgcca acgaccagac
     1801 ggccatcggc gccgcgctgg gcctgtaccg gcgcaacgtg cgcgtgcccg acgacgtctc
     1861 gctcgtgggc ttcgacgacc tggcgcccgc caagttctcg gtgccgccgc tgacgacgat
     1921 ccggcagtcg gtctacgaga tcggctccga ggccgcggcc gcgatgctgg cgatgctcaa
     1981 gggcgaagag cccggcgccg acctgccgcc gccggaactg gtggcgcgcg agtccacgcg
     2041 gcgcgtgctg cgctgagcgt cgatcccttg gcggtgccag gtcttgcctg gcaatcggcg
     2101 gtgccaggtc ttgcctggca aagccacgct tgacgagttg cctggcaaga cctggcaccg
     2161 ctgcatgacg agttgcctgg caagacctgg cacccatgga gggcgggcaa gccgcgaggc
     2221 cgccgttgcg gcgaagcggc gcgccgcgtg gccacgtcgt ggtgccgtcc tgcgcgcagt
     2281 cgcagctccg cacgcgccga tccattcgtt cgaacgtagg cgatgggtcc ttacgccgcg
     2341 atgcgtgccc ggaggggccg gtaggcacgg acgagttatc atgaaagcgc ttgcagccga
     2401 cgatccgacg gcgtcgacgc ctcgcgacgc ccggctttgc ctcgtgggca gctcatcttc
     2461 gggtttacgc tgtaacggct cggcagtggc tcgttgaaca atgttgaaag cgctttcaag
     2521 gcacctgccg gagcgtgaca ccccgttccg ataaaaagac cgtcccgaga caacccatgt
     2581 cgacgcctgc ctcgcgcgcc cgctccagcg cctccgcgcc cccgccgggc cgtccgcaca
     2641 tccgatgacc tcttccgacg attcccagcg cggcccccgc acgttcgcga cccgccgccg
     2701 cagcgtgctg gccgcgggcc tggccgccgc catcgcgccg gccgtgcgcg ccaccgagca
     2761 gcgtacgctg acggtcgccg cgttcccgct cgtcgaccag atcgtcaagg acgccatccc
     2821 gcaatggcag aagcgccacc cggacgtggc catcgacatc gtcatgcggc agtacgacga
     2881 ccaccacacg gcgatgacga cggcgctgtc gaccgcggtc tacctgcccg acgtgatggc
     2941 gctcgaggcg tcgtacgtcg gcaagttctc cgatggcggc ggcttgtccg acctgctgca
     3001 gccgcgctac gacatcggcc gctaccggga tcgcttcgtc gcctatgcct atgaccaggc
     3061 gatcaaccgc aagggccagg tggtggccgc gccgaccgac atcggcccgg gcacgatgct
     3121 ctatcgccag gacctgctcg agcgcgcggg tctcgtggcg agcgacctgc agccgtcgtg
     3181 ggacgcgtac atcgacgcgg gcatcaggat caagaaggcg acgggcgcca acctgatcgg
     3241 caacgtccgc accttcaagg acctggtcgt gcgcggcggc ctcaagaagg gccagggcat
     3301 ctacttcgac agcgactcga acgtgctggt ggacacgccg cgcttccatc gcgcattcga
     3361 gctgtcgctg cgcatgcgac gcaacgggct ggagggccac ctcgagacct ggagcaacga
     3421 atgggccgag gcgttccgcc gcaacgcgac cgccaccgaa ctgggcggct cgtggatggt
     3481 ggggcaactg gccaactggg tcgcgcccgc gaccaagggc ctgtggcgcg ctgcgcaact
     3541 gcccgagagc acctgggtgt cgtacggcgg cacctactac gcgatcccgc gcgattccga
     3601 cccgacccgc aaggcgctgt ccgacgtggc gcgactcctc gagctgcgcg ctcgccgctg
     3661 atcatgagac tgccatcctt gctccggctg tcgcccaagt gggcgccgta cgtgttcatc
     3721 agcccgttcc tcgtgctgtt cggcgtgt
//
LOCUS       DATCRO010000330        12986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_768939, whole genome shotgun sequence.
ACCESSION   DATCRO010000330 DATCRO010000000
VERSION     DATCRO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768939"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..823
                     /locus_tag="VIN75_08905"
     CDS             134..823
                     /locus_tag="VIN75_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HEY8974324.1"
                     /translation="MFTGIVQAVATVSVLVDKPGLRSFTLAFPRGFCQGLEIGASVSV
                     DGVCLTVTELAGEDAASFDVMQQSLSLTTLDALEVGSRVNVERAARDGAEIGGHPLSG
                     HVDFQARLASVRNPENNHVMRIAVPSPWMRYIFPKGYIAINGASLTVSDANKAEGWFE
                     VWLIPETLRMTTFGEKTAGAALNIEIERSTQVMVDTVRDAVDERLGALLPALTDLLRE
                     RGVDVDALLKG"
     gene            823..1506
                     /locus_tag="VIN75_08910"
     CDS             823..1506
                     /locus_tag="VIN75_08910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8974325.1"
                     /translation="MSARTVPDADAYRPPPDTGLEVLHVEPAFVVLAKPPGLLSEPGR
                     GPDKRDSLLVRAQAVYPDARIVHRLDMMTSGVIVLAREALAHASLSEAFREREVDKRY
                     EALVHGRPAADEGEIDLPLVVDWPNRPRQVVCHDTGKPSLTHYRVAGQVRVDGVGTLT
                     RIALTPVTGRTHQLRVHLASLGCPIAGDPFYGVADDPSPRMMLHACRLALAHPLTGEP
                     LVFVSAVPF"
     gene            1632..2753
                     /locus_tag="VIN75_08915"
     CDS             1632..2753
                     /locus_tag="VIN75_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8974326.1"
                     /translation="MPQAVISGTGLYQPPHVVTNEELVAAYNAYAERQNAINADAIER
                     GEKTALTASSVEFIEKASGIKQRYVIEKDGVLDPARMRPRFTERGDDELSLMAEIGAD
                     ACRKALAAAGKTGADVDAVLCAASNMQRAYPAMAIEIQQAIGAGGYGFDMNVACSSAT
                     FGIEQAVNAVRAGSARCVLVVNPEITSAHIEYKDRDCHFIFGDVCTATVVESAETATS
                     ADQWEIVGTRLATQFSNAIRNNFGFMNRSEDSDPAARDKTFRQEGRKVFKEVVPMAAA
                     HIEAHLQSLGLEPTQMRRFWLHQANLGMNQLILKKLVGAEAGGDMAPLVLDTYANTSS
                     AGSIIAFHAYHADMKPGELGVICSFGAGYSIGSVIVRKK"
     gene            2954..5317
                     /locus_tag="VIN75_08920"
     CDS             2954..5317
                     /locus_tag="VIN75_08920"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEY8974327.1"
                     /translation="MSPIRAVHAAVAAAWIALAAWPLAAAAEVGAQAEARAVQPAPEA
                     ALSPLTGHVDPITSDPLAMHAVRLLPDEHIVLDGTLSNPAWQRAPVYDNFVENEPHRD
                     AKPRYATKVQILFDEQALYVGVTSLDDHPEEIRAPLVRQDKVIRTQDFVVVYIDAIGK
                     RQSAQFFRVNASGSTGDGMHTAADDDEDFSPDFDFDAASARTSSGYTSVFRIPFASLR
                     FSSDSHAHWRIMVARRMPRDQAYMWTSVKVPTDAPAFISAMQPLEGIELPQQKNFLTI
                     RPSITARHTSDTEPGQARHTADGVQGSLDVKWRASPELVIDGTLKPDFSQVALDVPQL
                     SGNTDFALYLPEKRPFFFESSDLLHSPTEAMYTRSFTAPRWGVRSTWRGASLAGTAFV
                     IDDKGGGETLLPYGYGTNTALQPGSRSLSARGLFDVNDDGRFEVGGILAARRYEGERG
                     DNTVAGPDVNWQLNDAWRIKAQWLRSQTTAQPFTDPAGDVSLVKGPATTGDSVFLKVM
                     RYTDRTQLEISDLDISPQFRHDTGFVNQVGVRDIEFHPSMVFRDLAPINEMWLNLRGS
                     LVRDRINGDVIQESLAPDVWVSLPHNTQAWISWHGLSRLRATAGGRLLTEHYWKGEYT
                     STLAQSIPLFDATVSLGRMADVTAGVVRPGGQVNLMLSTRPFAAIEIEPSVWAAWLRN
                     GPLLAYRETASQLLGVWHIDARQNLRLIVQRSTVDRKPEAGVAADRESGLTTSLTYAF
                     RKSMGTVMYVGATRSHSGVGAQNIARGTEAFVKLQVDVDEVRGMFGH"
     gene            complement(5569..6069)
                     /locus_tag="VIN75_08925"
     CDS             complement(5569..6069)
                     /locus_tag="VIN75_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8974328.1"
                     /translation="MKAKTTPTAAELAQLDAYHAQVRGHLDRLAALASRVENGQLDAE
                     TSAEACAVEAFFSGAMQEHHRVEESGVFAFLLASDDKELVDVVRTLQQDHGWMEQNWI
                     ELAPQLRAIAQGNAWVDPAEFMHATEVFLELSYGHSALEEAVVYPRAQAARDAQADRL
                     VAGTPG"
     gene            6238..8061
                     /locus_tag="VIN75_08930"
     CDS             6238..8061
                     /locus_tag="VIN75_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974329.1"
                     /translation="MQFANSASPDYFHKVVDCQWACPAHTPVPEYIRLIGQGRYDDAY
                     MVNWVSNVFPGILGRTCDRPCEPACRRGRVEESNRDKPEPVAICRLKRVAADHKGDVR
                     ARMPSVAPLNGRRIACVGAGPASLTVARDLAPWGYEVTVFDGEARAGGFIRSQIPHFR
                     LPESVIDEETGYILDLGVKFEAGRRIDSMKALLALGYDAVFVGCGAPRGRDLDLPGRR
                     EAAENIHIGIDWLASVSFGHVTSVGERVIVLGGGNTAMDCCRSARRLGGREVTVIVRS
                     GFAEMKASPWEKEDAMHEGIPILDLHVPKSFEHVDGKLTGMTFEKVVARVDAQGRRQL
                     VPTGEPDVLVPCDTVLVAVGQENAFPWIEPDCGLLFDRHGLPVLDKATFQSSLPHVFF
                     GGDAAFGPKNIISAVAHGHDAAVSIDRLLNGEDVRLRPPPMSTLMSQKMGIHEWSYRN
                     DVSLDTRFKVPWAAAERALADIRVEVELGFDAATAYKEVERCLNCDVQTVFNRDTCIE
                     CDACVDICPMDCITFTGAGDEADLRTRLKAPALNLAQDLYVSQELKTGRVMVKDEDVC
                     LHCGLCAERCPTGAWDMQKFLFETTQAGPRARDARTNAEAA"
     gene            8058..9893
                     /locus_tag="VIN75_08935"
     CDS             8058..9893
                     /locus_tag="VIN75_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY8974330.1"
                     /translation="MKTIQAVNDFVIKFANVNGSGSASANELFAKAVMRMGVPVSPRN
                     IFPSNIQGLPTWYEARVCEAGWSGRRGGVDMMVAMNPQTWDADVAEIESGGYLFHDST
                     RPMPPNRFRDDVQVIGIPLTAIANAAYDEARQRQLFKNIIYVGALCALLDMEPAVFEK
                     LLAEQYKGKERLLDSNIRALHMGRDHVLQNLPHPLGIRVQRADRVGDSIFVDGNSAIA
                     LGCVYGGATVAAWYPITPSSSIPEAFQKYCDKYRVDAETGQHRFAIVQAEDELASIGI
                     AVGAGWNGARAFTATSGPGISLMQEFIGLAYFAEIPVTIIDVQRGGPSTGMPTRTQQS
                     DLLACAYASHGDTKHVLLFPEDPHECFDHAAAALDLADRLQTPVFVMTDLDIGMNQRL
                     ARPFAWDAAREYDRGKVMTAEELEAGANFGRYKDVDGDGIAWRTLPGTHPSKGAFFTR
                     GTTRDPYARYSEKGPDYIYNMERLQRKFRTAASLVPQPVLRPARAATRVGAIYFGSTS
                     PVMAEALDVLESEDIHIDALRLRAFPFPQAVRDFIAAHDKVFVVEQNRDAQMHLMLVN
                     ELDVDPERLVRVLHYDGTPINARFIAGAIRNSLPHAMARGQEMAK"
     gene            9890..10945
                     /locus_tag="VIN75_08940"
     CDS             9890..10945
                     /locus_tag="VIN75_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY8974331.1"
                     /translation="MTYIAKPLLRHPTLHENQVGYTRRDYEGRVSTLCAGCGHDSISA
                     AIVQACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANLANR
                     DLLYLGVSGDGDSASIGLGQFANALRRGVRMAYIVENNGVYGLTKGQFSATADRGSKS
                     KKGVLNTDNPIDLVGIALQLGATYVARSFSGDKEQLVPLVKGAMSHGGAAFIDVISPC
                     VTFNNHAGSTKSFDYVRQHNEAVSRIDFMAPGEQITTSCAPGEAVDVPQHDGSVLRLR
                     KLHADYDATDRYAALSYMQQHAAQGEIVTGLLYLDPLAGDLHASLNTVAAPLQSLGRD
                     TLCPGSAVLDRINAGLR"
     gene            10987..11427
                     /locus_tag="VIN75_08945"
     CDS             10987..11427
                     /locus_tag="VIN75_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8974332.1"
                     /translation="MSERTVLQSLSRGHVLSLGPQASVRDAACAMTRANCGSVLIIDG
                     AGPMVGILTERDLMTRVLAKGLDADATTVTQVMTRNPRCVAPETKVADAVVIMMERGF
                     RHLPVVGEGGRVLGVFSVRDALPREVNTAVDLAEFNEQVNDALG"
     gene            complement(11515..12729)
                     /locus_tag="VIN75_08950"
     CDS             complement(11515..12729)
                     /locus_tag="VIN75_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974333.1"
                     /translation="MSNTACTPLATLPAVPGRMSRSAALWLNASILVAYLAASTAPSP
                     LYALYREAWGFSALTLTVVFSTYAFALLGALLFFGALSDHRGRREVVLVSLGLEIASL
                     LVFRHADSVAWLIVARAVQGVATGIATSALSAGMLDISRERGSLLNSVAPLVGMGVGA
                     LGAGALAQFAPAPTRLVFDVLLAILAAQALAAFFLPETVSKRAGALAAMKPQLAVPAR
                     AWPTLWQVLPVNTAQWALGGFYAPLGPSLARIVTGIHSPLIGGGVVATLVLAGAAAVL
                     AVRARPARAVLSGGTAALVVGLVVTLAGVELHSTAMFFAGSFIAGLGFGAAFNGTMRS
                     LVPLAEAHERAGLMSTFFVLSYLAFSLPAILAGLMAGRFGLQAASVGYGLLLVTLGAV
                     ALAMMARRKGAA"
     gene            12829..>12986
                     /locus_tag="VIN75_08955"
     CDS             12829..>12986
                     /locus_tag="VIN75_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8974334.1"
                     /translation="MTTTSRAIPRPGGRSARVQAAVHQATLDLLKQHGRAGLTVPLIA
                     ARAGVTPST"
BASE COUNT         2061 a         4677 c         4451 g         1797 t
ORIGIN      
        1 gtcttgattc gtccagcgcc tccctccaga gtgcatgatt caagacctga caccgcaact
       61 ttcaagacct gacacccgcg gggggaggcg cgagcagcga caatcccggc ttcgtccacc
      121 aggaagctcc gccatgttca ccggcatcgt ccaggccgtc gccaccgtct ccgtgctcgt
      181 cgacaagccg ggcctgcgca gcttcacgct cgccttcccg cgcggcttct gccaaggcct
      241 ggagatcggc gccagcgtgt cggtggacgg cgtatgcctg accgtgacgg agctcgcggg
      301 cgaggacgcc gccagcttcg acgtgatgca gcagagcctg tcgctcacga cgctcgacgc
      361 gctcgaggtc ggcagccgcg tcaacgtcga gcgcgcggcg cgcgacggcg cggagatcgg
      421 cggccacccg ctgtcgggcc acgtcgactt ccaggccaga ctcgcctcgg tgcgcaaccc
      481 cgagaacaac catgtcatgc gcatcgccgt gccgtcgccg tggatgcgct acatctttcc
      541 caagggctac atcgccatca acggcgccag cctgacggtg tccgatgcga acaaggccga
      601 aggctggttc gaggtttggc tgatccccga gacgctgcgc atgaccacct tcggcgagaa
      661 gaccgcgggc gccgcgctca acatcgagat cgagcgcagc acccaggtga tggtggacac
      721 cgtgcgcgac gccgtcgacg agcgcctggg cgcgctgctg ccggccttga ccgacctgct
      781 gcgcgagcgc ggcgtcgacg tcgacgcgct gctcaagggt tgatgtccgc ccgcaccgtt
      841 cccgacgccg acgcctaccg gccgccgccg gacaccggcc tggaggtgct gcacgtcgag
      901 ccggccttcg tggtgctcgc caagccgccc gggctgctgt ccgagccggg ccgcggaccg
      961 gacaagcgcg acagcctgct ggtccgcgcg caggccgtgt acccggatgc gcgcatcgtc
     1021 catcgcctgg acatgatgac cagcggcgtc atcgtgctgg cgcgggaggc gctcgcgcac
     1081 gcgtcgctca gcgaggcctt ccgcgagcgc gaggtcgaca aacgctacga ggcgctggtg
     1141 cacggccgcc ccgcggccga cgagggcgag atcgacctgc cgctggtcgt cgactggccc
     1201 aaccgcccgc gccaggtcgt gtgccacgac accggcaagc cgtcgctgac gcactaccgc
     1261 gtggctggac aggtgcgggt cgacggcgtg ggcacgttga cgcgcatcgc gctcacgccg
     1321 gtcaccggcc ggacgcacca gttgcgcgtg cacctcgcgt cgctcggctg cccgatcgcg
     1381 ggcgacccgt tctacggcgt cgcggacgac ccgtcgccgc gcatgatgct gcacgcctgc
     1441 cggctcgcgc tggcgcatcc gctcacgggc gagccgctgg tcttcgtgag cgcggtgccg
     1501 ttctgagctg gcgcgtcaac ctgcgcgatc ttgccggcaa cgtcgcgcgt cctgggtgct
     1561 ttccccgaga acgcacgccg atcacgccgt gccccgccag ctaaaatcgg ccggttcgaa
     1621 caggaatctc catgccacaa gcagtcatca gcggcaccgg cctctaccag ccgccgcacg
     1681 tcgtcaccaa cgaggaactc gtcgccgcct acaacgcgta cgccgagcgg cagaacgcga
     1741 tcaacgccga cgccatcgag cgcggcgaga agaccgcgct gacggcctcc agcgtggagt
     1801 tcatcgagaa ggcctcgggc atcaagcagc gctacgtcat cgagaaggac ggcgtgctgg
     1861 acccggcccg catgcgcccg aggttcaccg agcgcggcga cgacgagctg tcgctgatgg
     1921 ccgagatcgg cgccgacgcc tgccgcaagg cgctcgcggc cgccggcaag acgggcgcgg
     1981 acgtcgacgc cgtcctgtgc gccgcgtcca acatgcagcg tgcctacccg gcgatggcga
     2041 tcgagatcca gcaggcgatc ggcgccggcg gctacggttt cgacatgaac gtggcgtgct
     2101 cgtcggccac cttcggcatc gagcaggccg tcaacgcggt gcgcgcgggc agcgccaggt
     2161 gcgtgctggt cgtgaacccg gagatcacgt cggcgcacat cgaatacaag gaccgcgact
     2221 gccacttcat cttcggcgac gtctgcacgg ccacggtggt cgagagcgcc gagacggcca
     2281 cctcggccga ccagtgggaa atcgtcggca ccaggctggc gacgcagttc tcgaacgcga
     2341 tccgcaacaa cttcggcttc atgaaccgca gcgaggattc cgatcccgcc gcgcgcgaca
     2401 agaccttccg ccaggagggc cgcaaggtgt tcaaggaggt cgtgccgatg gccgcggcgc
     2461 acatcgaggc gcacctgcag agcctgggcc tggagccgac gcagatgcgc cgcttctggc
     2521 tgcaccaggc caacctcggc atgaaccagc tgatcctcaa gaagctggtc ggcgcggagg
     2581 ccggcggcga catggcgccg ctggtgctcg acacctacgc caacacctcg tcggccggct
     2641 ccatcatcgc cttccacgcg taccacgccg acatgaagcc gggcgagctc ggcgtgatct
     2701 gctcgttcgg cgcgggctac tccatcggca gcgtgatcgt ccgcaagaag tgatgcgcct
     2761 cccggcgccg accgcacaag gggcccgcgt ggccccttcc tgttgcccga aaaccgtcat
     2821 tcgtcgcgtc gggtgcccgc cgacccccgg ggctcgctga caatcccgtc agaaacgcgg
     2881 cctggctgac ggccgcccca tgacgaacga agaacaggcg cggccgccga ggcggccggc
     2941 gcggggaagg tccatgagtc cgattcgagc agtgcatgcc gccgtggccg cggcatggat
     3001 cgcgctggcc gcgtggccgc tcgcggccgc cgcggaggtg ggcgcgcagg ccgaggcgcg
     3061 cgcggtccag ccggcgcccg aggccgcgct gtcgccgctg acgggccacg tcgacccgat
     3121 cacgagcgat cccctcgcca tgcacgccgt gcgcctgctg cccgacgaac acatcgtcct
     3181 ggacggcacg ctgagcaacc cggcctggca gcgcgcgccg gtctacgaca acttcgtcga
     3241 gaacgagccg caccgcgacg ccaagcccag gtatgccacc aaggtgcaga tcctgttcga
     3301 cgagcaggcg ctgtacgtgg gcgtcacgtc gctggacgac caccccgagg agatccgcgc
     3361 accgctggtg cgccaggaca aggtgatccg cacgcaggac ttcgtggtcg tctacatcga
     3421 cgcgatcggc aagcgccagt cggcgcagtt cttccgcgtc aatgcgtccg gctcgaccgg
     3481 cgacggcatg cacacggccg ccgacgacga cgaggacttc tcgcccgact tcgatttcga
     3541 cgccgcctcc gcgcgcacgt ccagcggcta cacctcggtg ttccgcatcc cgttcgcgtc
     3601 gctgcgcttc tcgtcggaca gccacgcgca ctggcgcatc atggtcgcgc gccgcatgcc
     3661 gcgcgaccag gcctacatgt ggacgtctgt gaaggtgccc accgacgcgc cggccttcat
     3721 ctcggcgatg cagccgctcg agggcatcga gctgccgcag cagaagaact tcctcacgat
     3781 ccgccccagc atcacggcgc gccacaccag cgacaccgaa cccgggcagg ccaggcacac
     3841 ggccgatggc gtgcagggca gcctggacgt gaagtggcgc gcgtcgcccg agctggtgat
     3901 cgacggcacg ctcaagcccg acttctcgca ggtggcgctc gacgtgccgc agctcagcgg
     3961 caacaccgac ttcgcgctgt acctgcccga gaagcggccg ttcttcttcg agtccagcga
     4021 cctgctgcac tcgcccaccg aggcgatgta cacgcgcagc ttcacggcgc cgcgctgggg
     4081 cgtgcgatcg acgtggcgcg gcgcgtcgct ggccggcacg gccttcgtca tcgacgacaa
     4141 gggcggcggc gagacgctgc tgccctacgg ctacggcacc aacaccgcgc tgcagccggg
     4201 ctcgcgctcg ctgtccgcgc gcggactgtt cgacgtcaac gacgacggac gcttcgaggt
     4261 cggcggcatc ctggccgcgc gccgctacga gggcgagcgc ggcgacaaca cggtcgccgg
     4321 gccggacgtc aactggcagc tgaacgacgc gtggcgcatc aaggcccagt ggctgcgctc
     4381 gcagacgacg gcgcagccgt tcacggaccc tgccggcgac gtctcgctgg tcaaggggcc
     4441 cgcgacgacc ggcgactcgg tgttcctgaa ggtgatgcgc tacaccgacc gcacgcagct
     4501 ggagatctcg gacctcgaca tcagcccgca gttccgccac gacaccggct tcgtcaacca
     4561 ggtgggcgtc cgcgacatcg agttccaccc gtcgatggtg ttccgcgacc tcgcgcccat
     4621 caacgagatg tggctgaacc tgcgcggctc gctggtgcgc gaccgcatca acggcgacgt
     4681 catccaggaa tcactggcac cggacgtgtg ggtgagcctg ccgcacaaca cgcaggcgtg
     4741 gatctcgtgg cacgggctgt ccaggctgcg cgccaccgcc ggcggccggc tgctgaccga
     4801 gcactactgg aagggcgagt acacgtcgac gctggcgcag tccattccgt tgttcgacgc
     4861 cacggtctcg ctgggcagga tggccgacgt cacggccggc gtcgtgcgac cgggcgggca
     4921 ggtcaacctg atgctgtcca cgcgcccgtt cgcggcgatc gagatcgagc ccagcgtctg
     4981 ggcggcgtgg ctcaggaacg ggccgctgct ggcctatcgc gagacggcct cgcagctgct
     5041 gggcgtgtgg cacatcgacg cgcgccagaa cctgcgcctg atcgtgcagc gctccaccgt
     5101 cgaccgcaag cccgaggccg gcgtcgcggc ggaccgcgag tcgggcctga cgacctcgct
     5161 gacctatgcg ttccgcaagt cgatgggcac cgtgatgtac gtgggcgcga cgcgctctca
     5221 cagcggcgtc ggcgcacaga acatcgcgcg cggcaccgag gccttcgtga agctgcaggt
     5281 ggatgtcgac gaggtgcggg gcatgttcgg gcactgatgt cggacgcggt cgcacgccgg
     5341 gcgatcgccg aaggggtctg gcctctcctc gaatcgcgct ggcggctcgt gccgagagtc
     5401 gaggacatgg ccgcgattcg tggcgaaggc ctgaccccga tcgtcagcga atgttcgggg
     5461 tcaggccttt gcctcgaatc gcgatccacg ctggcgctgc gatgagcgag tcggcgcgat
     5521 ccgaggagag gccagacccc tctggcctcg gcgcgccacc cgaagtgttc aacccggcgt
     5581 gcccgccacc agccggtcgg cctgggcgtc ccgcgccgcc tgcgcgcgcg ggtagacgac
     5641 ggcctcctcg agcgccgagt gaccgtagct cagctccagg aacacctcgg tcgcgtgcat
     5701 gaactcggcc gggtcgaccc aggcgttgcc ctgggcgatg gcgcgcagct gcggcgcgag
     5761 ctcgatccag ttctgctcca tccagccgtg gtcctgctgc agcgtgcgaa cgacgtcgac
     5821 gagttccttg tcgtcgctgg ccagcaggaa ggcgaacacg ccggactcct cgacgcggtg
     5881 gtgctcctgc atggcgccgg agaagaaggc ctcgacggca caggcctcgg cgctggtctc
     5941 cgcgtcgagc tggccgtttt ccacccggga ggcgagggcg gccaggcgat ccaggtggcc
     6001 gcggacctgg gcatggtatg cgtccagctg ggcgagttcg gcggccgtgg gcgtggtctt
     6061 ggctttcatc gcgggctcct ggacgacgga tgcttccttt atacgccgcc ggacgggcgg
     6121 cgtgggcgct ttcgcggcaa cgtttgacct gagcccaggg ccaacgcgat cggcctagac
     6181 tcgggaggcc ccgtccggcg catgctcgtg cccgacccgc gcgaaggaga ctgcaacgtg
     6241 caattcgcca acagtgcctc gcccgactat ttccacaagg tggtcgattg ccagtgggcc
     6301 tgcccggcac atacgccggt gcccgagtac atccggctga tcgggcaagg gcgctacgac
     6361 gacgcctaca tggtcaactg ggtgtccaac gtcttcccgg gcatcctcgg gcgcacctgc
     6421 gaccgcccat gcgagccggc ctgccggcgc gggcgcgtgg aggaaagcaa ccgcgacaag
     6481 cccgagccgg tggccatctg ccggctcaag cgcgtggccg ccgaccacaa gggcgacgtg
     6541 cgcgcccgca tgccgtcggt ggcgccgctc aacggccggc gcatcgcctg cgtcggcgcc
     6601 gggccggcct cgctgacggt ggcgcgcgac ctggcgccct ggggctacga agtcaccgtg
     6661 ttcgacggcg aggcccgcgc cggcggcttc atccgctccc agatcccgca cttccggctg
     6721 cccgagtcgg tcatcgacga ggagacgggc tacatcctgg acctgggcgt gaagttcgag
     6781 gcgggccgcc gcatcgactc gatgaaggcg ctgctcgcgc tcggctatga cgcggtgttc
     6841 gtcggctgcg gcgcgccgcg cgggcgcgac ctcgacctgc ccggccggcg cgaggcggcc
     6901 gagaacatcc acatcggcat cgactggctg gcctcggtct cgttcggcca cgtcaccagc
     6961 gtgggcgagc gcgtgatcgt gctgggcggc ggcaacaccg cgatggattg ctgccgatcc
     7021 gcgcggcgcc tcggcggccg cgaggtgacc gtgatcgtgc gcagcggctt cgccgagatg
     7081 aaggcctcgc cgtgggagaa ggaggatgcg atgcacgagg gcatccccat cctcgacctg
     7141 cacgttccga aatccttcga gcacgtggac ggcaagctga ccggcatgac cttcgagaag
     7201 gtggtcgcaa gggtcgacgc ccagggccgg cgccagctgg tgcccaccgg cgaaccggac
     7261 gtgctggttc cctgcgacac cgtcctggtg gccgtcggcc aggagaacgc gttcccgtgg
     7321 atcgagcccg actgcggcct cctgttcgac cgccacggcc tgccggtgct cgacaaggcc
     7381 acgttccagt cctcgctgcc ccacgtgttc ttcggcggcg atgcggcgtt cgggccgaag
     7441 aacatcatct cggcggtcgc gcacgggcac gacgccgcgg tgtcgatcga ccgcctgctc
     7501 aacggcgagg acgtgcggct gcggccgccg cccatgtcga cgctgatgtc gcagaagatg
     7561 ggcatccacg aatggagcta ccgcaacgac gtgtcgctgg acacgcgctt caaggtgccc
     7621 tgggccgcgg ccgagcgcgc gctcgccgac atccgcgtgg aggtcgagct cggcttcgac
     7681 gccgccaccg cgtacaagga agtggagcgc tgcctgaact gcgacgtgca gaccgtcttc
     7741 aaccgcgaca cctgcatcga gtgcgacgcc tgcgtcgaca tctgccccat ggactgcatc
     7801 acgttcaccg gcgcgggcga cgaggccgac ctgcgcacgc ggctgaaggc gccggcgctc
     7861 aacctcgcgc aggacctgta cgtctcgcag gagctcaaga cgggccgcgt gatggtcaag
     7921 gacgaggacg tctgcctgca ctgcggactt tgcgccgagc gctgccccac cggcgcatgg
     7981 gacatgcaga agttcctgtt cgagacgacg caggcgggcc cgcgcgcccg tgacgcgcgc
     8041 acgaacgcgg aggccgcatg aagaccatcc aggccgtcaa cgacttcgtc atcaagttcg
     8101 ccaacgtcaa cggctcgggt tcggcctcgg ccaacgagct gttcgccaag gccgtgatgc
     8161 gcatgggcgt gccggtgagc ccgcgcaaca tcttcccgag caacatccag ggcctgccca
     8221 cctggtacga ggcgcgcgtg tgcgaggccg ggtggtcggg ccggcgcggc ggcgtcgaca
     8281 tgatggtggc gatgaacccg cagacctggg acgccgacgt cgcggagatc gagtccggcg
     8341 gctacctgtt ccacgactcg acgcggccga tgccgcccaa tcgcttccgc gacgacgtcc
     8401 aggtgatcgg catcccgctg accgcgatcg ccaacgccgc ctacgacgaa gcgcgccagc
     8461 gccagctgtt caagaacatc atctacgtcg gcgcgctgtg cgcgctgctg gacatggagc
     8521 cggcggtctt cgagaagctg cttgccgagc agtacaaggg caaggagcgg ctgctcgact
     8581 ccaacatccg cgcgctgcac atgggccgcg accacgtgct gcagaacctg ccgcatccgc
     8641 tgggcatccg cgtgcagcgg gccgatcgcg tcggcgactc gatcttcgtc gacggcaact
     8701 cggcgatcgc gctgggctgc gtctacggcg gcgcgacggt ggcggcgtgg tatccgatca
     8761 cgccgtcgtc ctcgatcccc gaggccttcc agaagtactg cgacaagtac cgcgtcgacg
     8821 cggagacggg ccagcatcgc ttcgcgatcg tccaggccga ggacgagctc gcgtcgatcg
     8881 gcatcgcggt gggcgccggc tggaacggcg cgcgcgcatt cacggccacc tcggggcccg
     8941 gcatctcgct gatgcaggag ttcatcgggc tcgcgtactt cgccgagatc cctgtcacga
     9001 tcatcgacgt gcagcgcggc ggcccgtcca ccggcatgcc cacgcgcacg cagcagtcgg
     9061 acctgctcgc ctgcgcgtac gcgtcgcacg gcgacaccaa gcacgtgctg ctgttccccg
     9121 aggatccgca cgagtgcttc gaccacgcgg ctgcggcgct cgacctcgcc gaccggttgc
     9181 agacgcccgt cttcgtgatg accgacctgg acatcggcat gaaccagcgc ctcgcccggc
     9241 cgttcgcctg ggacgccgcg cgcgagtacg accgcggcaa ggtgatgacg gccgaggagc
     9301 tcgaggccgg cgcgaatttc ggccgctaca aggacgtcga cggcgacggc atcgcgtggc
     9361 gcacgctgcc cggcacgcac ccgagcaagg gcgcgttctt cacccgcggc acgacgcgcg
     9421 acccgtacgc gcgctactcc gagaaagggc ccgactacat ctacaacatg gagcggctgc
     9481 agcgcaagtt ccgcacggcg gcgtcactcg tgccgcaacc ggtgctgcgt cccgcgcgcg
     9541 ctgcgacgcg ggtcggcgcg atctacttcg ggtcgaccag cccggtgatg gccgaggcgc
     9601 tcgacgtgct ggagtccgag gacatccaca tcgacgcgct gcggctgcgc gcgttcccgt
     9661 tcccgcaggc ggtgcgcgac ttcatcgcgg cgcacgacaa ggtgttcgtc gtcgagcaga
     9721 accgcgacgc gcagatgcac ctgatgctgg tcaacgagct cgacgtcgac cccgagcgcc
     9781 tcgtgcgcgt gctgcactac gacggcacgc cgatcaacgc gcgcttcatc gccggcgcga
     9841 tccgcaacag cctgccgcac gccatggcgc gcggccagga gatggccaaa tgacctatat
     9901 cgccaagccg ctgctgcgcc acccgacgtt gcacgagaac caggtcgggt acacgcggcg
     9961 cgactacgag gggcgcgtct ccacgctgtg cgcgggctgc gggcacgact cgatctccgc
    10021 ggccatcgtg caggcctgct gggaactgga catcgaaccg caccgcgtgg ccaagctctc
    10081 gggcatcggc tgcagctcca agacgccgga ctacttcctg ggcgcctcgc acggcttcaa
    10141 caccgtgcac ggccgcatgc cgtcggtgct caccggcgcc aacctcgcca accgcgacct
    10201 gctgtacctg ggcgtctcgg gcgacggcga ttcggcctcg atcggcctgg gccagttcgc
    10261 caacgcgctg cggcgcggcg tgcgcatggc ctacatcgtc gagaacaacg gcgtctacgg
    10321 cctgaccaag ggccagttct cggccaccgc cgaccgcggc tccaagagca agaagggcgt
    10381 gctcaacacg gacaacccga tcgacctggt gggcatcgcg ctgcagctgg gcgccaccta
    10441 cgtcgcgcgc agcttctcgg gcgacaagga gcagctggtg ccgctggtca agggcgcgat
    10501 gtcgcacggc ggcgcggcgt tcatcgacgt catcagtccc tgcgtcacgt tcaacaacca
    10561 cgccggctcg accaagagct tcgactacgt gcgccagcac aacgaggccg tcagccgcat
    10621 cgacttcatg gcgcccggcg agcagatcac gacgagctgc gcccccggcg aggccgtcga
    10681 cgtgccgcag cacgacggca gcgtgctgcg cctgcgcaag ctgcacgccg actacgacgc
    10741 caccgaccgc tacgccgcgc tcagctacat gcagcagcat gccgcgcagg gcgagatcgt
    10801 caccgggctg ctgtacctgg acccgctcgc gggcgacctg cacgcgtcgc tcaacacggt
    10861 cgccgcgccg ctgcagtcgc tcgggcgcga cacgctgtgc ccgggcagcg ccgtgctcga
    10921 caggatcaac gccggcctgc gctgaggccg cccgccctcg aacgtccacg tgcaaggagc
    10981 tccgccatgt ccgaacgcac cgtgctccag tccctgtccc gcggccacgt cctgagcctg
    11041 ggcccgcagg ccagcgtgcg cgacgcggcc tgcgcgatga cgcgcgccaa ctgcggcagc
    11101 gtcctcatca tcgacggcgc cgggccgatg gtgggcatcc tcaccgagcg cgacctcatg
    11161 acgcgcgtgc tggccaaggg cctggacgcc gacgccacga ccgtcacgca ggtgatgacg
    11221 cgcaacccgc gctgcgtggc gcccgagacg aaggtggccg acgccgtcgt gatcatgatg
    11281 gagcggggct tccgccacct gcccgtggtg ggcgagggcg ggcgcgtgct gggcgtcttc
    11341 tcggtgcgcg atgcgctgcc gcgggaggtg aacacggcag tggacctggc ggagttcaac
    11401 gagcaggtga acgacgcgct ggggtgagcg cgaagcgacg acatcgcgtc tcgcggtgcg
    11461 tggatcccgc gacttcgcgc aggacgacag gtttgagcgg tgtccggcgt cgcgtcaggc
    11521 cgcccccttc cgccgcgcca tcatcgccag cgcgacggcc cccagcgtca ccagcagcag
    11581 cccgtagccc accgaagcgg cctgcagccc gaagcgtccc gccatcagcc cggccaggat
    11641 cgccggcagg ctgaacgcga ggtagctcag cacgaagaac gtcgacatca ggccggcgcg
    11701 ctcgtgcgcc tcggccagcg ggaccaggct gcgcatcgtg ccgttgaacg cggcgccgaa
    11761 gcccaggccg gcgatgaacg agccggcgaa gaacatcgct gtggaatgca gttccacgcc
    11821 ggccagcgtc acgaccagcc cgaccaccag cgccgccgtg ccacccgaca gcacggcgcg
    11881 ggccggtcgc gcgcgcaccg cgagcaccgc ggccgcgccg gcgagcacca gcgtggccac
    11941 cacgccgccg ccgatcagcg gcgagtggat gcccgtgacg atgcgcgcca gcgacgggcc
    12001 cagcggcgca tagaagccgc ccagggccca ctgcgcagtg ttgaccggca gcacctgcca
    12061 cagcgtgggc cacgcacgcg ccggcacggc cagctgcggc ttcatcgcgg ccagcgcgcc
    12121 ggcccgcttc gagaccgtct cgggcaggaa gaacgccgcg agcgcctgcg ccgccaggat
    12181 cgcgagcagc acgtcgaaca ccagtcgcgt cggggcgggc gcgaactgcg ccagcgcgcc
    12241 ggcgccgagc gcgccgacac ccatgcccac cagcggcgcg acgctgttca gcagcgagcc
    12301 ccgctcgcgg gagatgtcca gcatgccggc gctcagcgcg ctggtcgcga tgccggtggc
    12361 cacgccctgc acggcgcgcg cgacgatgag ccaggcgacg gagtccgcat gccggaacac
    12421 cagcagcgag gcgatctcca gtcccagcga gaccagcacc acttcgcggc gaccgcggtg
    12481 gtcggacagc gcgccgaaga acagcagcgc gcccagcagc gcgaacgcgt aggtcgagaa
    12541 gaccacggtg agcgtgagcg ccgagaagcc ccaggcctcg cggtacagcg cgtacagcgg
    12601 cgacggcgcg gtcgacgccg ccaggtaggc gacgaggatg gacgcgttga gccacaacgc
    12661 ggcgctgcgg ctcattcggc cggggacggc gggcagggtg gcgagcggcg tgcaggcggt
    12721 gttcgacatg gtgggctccg actcggaaca aacgcaaagt ttttgcgtta gcgaattctg
    12781 caccccttcg gtcgctaatg caattccttt gctttaagat gggttcccat gacgacgact
    12841 tcccgcgcga ttccccgtcc cggcggccgc agcgcccgcg tccaggcggc ggtgcaccag
    12901 gccacgctcg acctgctcaa gcagcacggc cgcgccgggc tgacggtgcc gctgatcgcc
    12961 gcccgggcgg gcgtcacgcc ctcgac
//
LOCUS       DATCRO010000331         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_588877, whole genome shotgun sequence.
ACCESSION   DATCRO010000331 DATCRO010000000
VERSION     DATCRO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588877"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VIN75_08960"
     CDS             <1..454
                     /locus_tag="VIN75_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974335.1"
                     /translation="PPLEARDLAARHLLATEDRFSALNTADLTAEAARNTEGATIEQQ
                     MDQALALSMTHAPGDLGRAQALLDQVLRNNTPQADPWRSLARLLAYRLGEQKRAEDAV
                     ERGAQQLRDAQRDNQRKLDDANAKLEALKAIERSLNTRQAPAASSPKP"
     gene            464..1834
                     /locus_tag="VIN75_08965"
     CDS             464..1834
                     /locus_tag="VIN75_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8974336.1"
                     /translation="MSTGAHILVVDDDPDLLKLLSMRLSGAGYRVTAAESAEAALVKI
                     AMERPQLVLSDVQLPGKDGLALFDAIRVQHPSLPVILLTAHGTIPDAVEATSRGVFTY
                     LTKPFDGKALLDVIANALAMEGAADTAADGGEIEAWRAEIVSRSTRMAEVLAEAKLVA
                     ASDASVLIRGESGSGKELIARAIHRASPRAAAPFVAVNCGAIPENLLESELFGHVKGA
                     FTGAVANHPGLFQAANGGTLFLDEIGDMPVALQVKLLRVLQERMVRPVGASHAVPVDV
                     RILSATHRDLDLAIIDGSFRKDLYYRLNVVALSLPTLAERREDIPLLASHFLSRLALK
                     YGRKVNGFAPDAMKALTTASWPGNVRQLHNVVEQVTALATTPLVPLALVQRALRVPSM
                     EVLSYTEARTRFERDYLVGLLKITDGNVADAARLADRNRTEFYRLMQKHGLTPGLFRG
                     DAPAAE"
     gene            2064..3614
                     /locus_tag="VIN75_08970"
     CDS             2064..3614
                     /locus_tag="VIN75_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEY8974337.1"
                     /translation="MHLPGRRKRPFPSVVLRSALACAALALASAQACAALFDDVGARA
                     RQLAEKPFVAQPKPTAPAAASDTLTYDQYRDIRFRPDHSLWRDQKLPFEVQFFHPGFV
                     NTETVKIHEVEPGGAAHEVRFDTADFDYGHNKPPSRALASGYSGFRVHFPINNANHKD
                     EVIVFQGASYFRAIGKDQRYGLSARGLAVDTVGAGAEEFPRFTEFWLEKPAADATSLV
                     ITALLDSKSVTGAYRFVVTPGQDTVVDVQARLYLRKDVKTLGIAPLTSMFFSGENQPQ
                     PGDFRPEVHDSDGLMVQTGTGEWLWRPLSNPRHILVTSFSAPSSIKGFGLMQRDRAYT
                     SYEDDEARYELRPSAWITPVGDWGPGRVELVQLNTVDETNDNIVAYWVPAKLPAPGAP
                     LDLAYRMRWQGDAEQHPPGAWVTQTRAGRSFATLAKGEEQLIVDFTGPSLAALPADAK
                     VEAVVTGVANVDVVSTNVYRNEANGTWRLALRFKQRKADEPVELRAFLQHGKDILSET
                     WTHVIPPR"
BASE COUNT          583 a         1336 c         1195 g          503 t
ORIGIN      
        1 cccgccgctg gaggcgcgcg acttggcggc ccgccacctg ctcgccaccg aggatcgctt
       61 cagcgcgctc aacacggccg acctgaccgc cgaggccgcg cgcaacaccg aaggcgcgac
      121 gatcgagcag cagatggacc aggcgctcgc gctgagcatg acgcacgcgc cgggcgacct
      181 cggccgcgcg caggcgctgc ttgatcaggt gctgcgcaac aacacgccgc aggccgaccc
      241 gtggcgctcg ctcgcgcgcc tgctggccta ccgcctgggc gaacagaagc gcgccgagga
      301 cgcggtcgag cgcggcgcgc agcagctgcg cgacgcccag cgcgacaacc agcgcaagct
      361 cgacgacgcc aacgccaagc tcgaggcgct caaggcgatc gagcgcagcc tcaacacgcg
      421 ccaggcgccg gcagcatcgt cgcccaagcc gtaagctgcg accatgagca ccggagccca
      481 catcctggtc gtcgacgacg accccgacct gctgaagctg ctgtcgatgc ggctgtccgg
      541 cgcgggctac cgcgtcaccg cggccgagtc cgccgaggcg gcgctggtca agatcgcgat
      601 ggagcggccg caactggtgc tcagcgacgt gcagctgccg ggcaaggacg ggctcgcgct
      661 gttcgacgcg atccgcgtgc agcacccttc gctgcccgtg atcctgctga ccgcgcacgg
      721 cacgatcccc gacgccgtgg aggccacgtc gcgcggcgtc ttcacgtacc tcaccaagcc
      781 gttcgacggc aaggcgctgc tggacgtcat cgccaacgcg ctggcgatgg aaggcgccgc
      841 cgacacggcg gccgacggcg gcgagatcga ggcctggcgc gccgagatcg tcagccgctc
      901 cacgcgcatg gccgaggtgc tggccgaggc caagctcgtc gccgcctccg acgcgagcgt
      961 gctgatccgc ggcgagagcg gcagcggcaa ggagctgatc gcgcgcgcca tccaccgcgc
     1021 cagcccgcgc gcggcggcgc cgttcgtggc cgtcaactgc ggcgcgatcc cggagaacct
     1081 gctcgagtcc gagctgttcg gccacgtcaa gggcgcgttc acgggcgcgg tggccaacca
     1141 tcccggcctg ttccaggcgg ccaacggcgg cacgctgttc ctcgacgaga tcggcgacat
     1201 gcccgtcgcg ctgcaggtca agctgctgcg cgtgctgcag gagcggatgg tgcggccggt
     1261 gggcgcgagc cacgcggtgc ccgtcgacgt gcgcatcctg tcggccacgc accgcgacct
     1321 cgacctggcg atcatcgacg gcagcttccg caaggacctg tactaccggc tcaacgtggt
     1381 cgcgctgtcg ctgcccacgc tggccgagcg gcgcgaggac atcccgctgc tggcgtcgca
     1441 cttcctgtcg cggctggcgc tcaagtacgg ccgcaaggtc aacggcttcg cgcccgacgc
     1501 gatgaaggca ctgacgaccg cgtcctggcc cggcaacgtg cgccagctgc acaacgtggt
     1561 cgagcaggtc acggcgctcg ccaccacgcc gctggtgccg ctggcgctgg tgcagcgcgc
     1621 gctgcgcgtg cccagcatgg aggtgctcag ctacaccgag gcgcgcacgc gcttcgagcg
     1681 cgactacctg gtcgggctgc tcaagatcac cgacggcaac gtggcggacg cggcccgcct
     1741 ggccgaccgg aaccgcaccg agttctaccg gctgatgcag aagcacggcc tgacgccggg
     1801 cctgtttcgc ggcgacgcgc cggccgccga gtagcagggc ttgtcgctga tcagcgacaa
     1861 gagaaatccc ttttgaatca acgacttgcg ttattgtgcg gtgcagcggt cgctgcggcc
     1921 cgacactttt cgaccatttc cgacagcgcg tcgcaagctt tccccacagg ggtgcggcaa
     1981 gtcattgatt ccaaacgact tttcgccctg gcacgtgcat tgcgaatgga gagcagacca
     2041 cctgcttacc agctcgtcag tacatgcatc ttccaggacg tcgaaagcgc cctttcccct
     2101 ctgttgtcct gcgttccgcc ctggcttgcg ccgcgctggc gctcgcgagc gcccaggcct
     2161 gcgcggcgct ctttgacgac gtcggcgcgc gtgcgcggca actggccgaa aagccgttcg
     2221 tcgcgcagcc gaagccgacc gcgcccgcgg ccgcgtcgga cacgctgacc tacgaccagt
     2281 accgcgacat ccgcttccgt cccgaccact cgctgtggcg cgaccagaag ctgccgttcg
     2341 aggtgcagtt cttccacccg ggcttcgtca acacggagac cgtgaagatc cacgaggtcg
     2401 agcccggcgg cgccgcgcac gaggtcaggt tcgacacggc cgacttcgac tacggccaca
     2461 acaagccgcc gtccagggcg ctcgcgtcgg gctactcggg ctttcgcgtc cacttcccga
     2521 tcaacaacgc gaaccacaag gacgaggtca tcgtcttcca gggcgcgagc tatttccgcg
     2581 cgatcggcaa ggaccagcgc tatggcctgt cggcgcgcgg cctggccgtc gacaccgtgg
     2641 gcgccggcgc cgaggagttc ccgcgcttca ccgagttctg gctcgagaag cccgccgccg
     2701 acgcgacctc gctggtgatc accgcgctgc tcgattcgaa gagcgtcacc ggcgcctacc
     2761 gcttcgtcgt cacgcccggc caggacaccg tcgtcgacgt gcaggcgcgc ctgtacctgc
     2821 gcaaggacgt caagacgctg ggcatcgccc cgctgaccag catgttcttc tccggcgaga
     2881 accagccgca gcccggcgac ttccgccccg aggtgcacga ctccgacggc ctgatggtgc
     2941 agacgggcac cggcgaatgg ctgtggcgcc cgctgagcaa tcccaggcac atcctcgtca
     3001 cctcgttttc cgcgccgtcg tcgatcaagg gcttcggcct gatgcagcgc gatcgcgcgt
     3061 acacgagcta cgaggacgac gaggcgcgct acgagctgcg ccccagcgcc tggatcacgc
     3121 cggtgggcga ctggggcccc ggccgcgtcg agctcgtcca gctcaatacc gtcgacgaga
     3181 ccaacgacaa catcgtcgcg tactgggtgc cggcgaagct gcccgcgccc ggcgcgccgc
     3241 tggacctcgc ctaccgcatg cgctggcagg gcgacgcgga gcagcacccc cccggcgcct
     3301 gggtcacgca gacgcgcgcg ggccgcagct tcgcgacgct ggccaagggc gaggagcagc
     3361 tcatcgtcga cttcaccggc ccgtcgctgg cggcgctgcc ggccgacgcc aaggtcgagg
     3421 ccgtcgtcac cggcgtcgcc aacgtcgacg tcgtgtcgac caacgtctac cgcaatgagg
     3481 ccaacggcac ctggcgcctg gccctccgat tcaagcaacg caaggccgac gagcccgtcg
     3541 agctgcgcgc gttcctccag cacggcaagg acatcctgag cgaaacatgg actcacgtca
     3601 tacctccccg atgaaca
//
LOCUS       DATCRO010000332         8444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_858644, whole genome shotgun sequence.
ACCESSION   DATCRO010000332 DATCRO010000000
VERSION     DATCRO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858644"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1568
                     /locus_tag="VIN75_08975"
     CDS             15..1568
                     /locus_tag="VIN75_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8974338.1"
                     /translation="MAEPLLRAVHEAGYTTPTPIQLQAIPVVLQGGDLLAGAQTGTGK
                     TAGFTLPMLHRLLEGGPKKDARGKPPVRALILTPTRELAAQVEESVRTYGKHVSLSSM
                     VIFGGVGMQPQINRLHKGVDILVATPGRLLDLHQQRAVDLSHIEIFVLDEADRMLDIG
                     FIHDIKKVLAVLPQKKQSLLFSATFSDEIKALADRLLNKPGLIEVARRNQTNEMIEQA
                     VHPVSRDRKKQLLSHLIKSGDWHQVLVFTRMKHGANRLAEFLNDDGITAMAIHGNKSQ
                     TARTRALSEFKAGTLQVLVATDIAARGIDIDQLPHVVNYELPNVPEDYVHRIGRTGRA
                     GAQGRAVSLVCVDEDIFLRDIEKLIKRPIPKEVVPGFEPDPGERAEPIVLGRTTIGVR
                     GGPSSGGRRDGGGHHARAGHGDRGGNGHGNGGGDRNRGQGRPGGAPGGRPAQQRPQGS
                     SGSHAGAKPAHGGAHAGHGGARPAGGQGHGNHGGGRPAGGGGGGRPQGGGQRAALTSP
                     SGGKGPVKR"
     gene            1747..2874
                     /locus_tag="VIN75_08980"
     CDS             1747..2874
                     /locus_tag="VIN75_08980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8974339.1"
                     /translation="MDLHTLFLAQTCLLAATAAMLWVSRSDADRGNGMQAWRLAITSQ
                     AVAYLVLAIPASGVAATFTGLVANLAGAVGVALYFIGVRRFAGLPVDRRPLAAMVAAV
                     TVAGAATGEHVAWAAIFNGFAYAGCQALAALTLWRAPRSGMVRVQQVVAAAYAAMAAV
                     LPLRAIALLAMRESRLDLRHDAWQVPIYVFGFLYLVVTNLGFILMCKMRAEAEIRLQA
                     RTDDLTGLPNRRALDEEIAHALAAARRSGRPFGVVMVDVDRFKFINDTHGHAVGDATL
                     SAFARRLASALREPDRAYRYGGEEFCVLLDDTDLAGARTLAERAREHIMLPFEGTMSA
                     LTASFGATAWQPDDTGDTLFRRADRALYAAKRNGRNRVETA"
     gene            complement(2933..3850)
                     /locus_tag="VIN75_08985"
     CDS             complement(2933..3850)
                     /locus_tag="VIN75_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY8974340.1"
                     /translation="MQTQSSTQNTGGKRQQYISVELRQWIVQQAQAGVAPETVLEAMK
                     SSGWDEDVAIQALEETLRSHLNQQVANQQAQQAAAPEGHEPQPVPVPDAAVRDQPSKL
                     RVLDRDVTVLSVMKLPRVIVLGGFLSDAECDQIIALAKPRMARSETVDNLTGGSEVNV
                     ARTSRGMFFERGETGVIDKVEKRIAALLDWPVQNGEGLQVLHYQPGAEYKPHYDYFDP
                     VHPGSAPILKRGGQRVGTVLMYLNTPRKGGGTTFPDVGFEVAPIKGNAVFFSYDRAHV
                     NTKSLHGGAPVIEGEKWVATKWLREREFV"
     gene            complement(4121..4939)
                     /gene="yaaA"
                     /locus_tag="VIN75_08990"
     CDS             complement(4121..4939)
                     /gene="yaaA"
                     /locus_tag="VIN75_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEY8974341.1"
                     /translation="MLLLLSPAKSLDYDSPLPEPVLAARAGLKPAATAPQYAAQAAEL
                     ISVLRTKTVADIADLMDLSEDLAKLNVKRYKAWSTRATERNSRPAMWAFNGDVYDGLR
                     ASTLNGDEIAWAQEHVLMLSGLYGVLRPLDRLQPYRLEMGTGLATERGRSLYAWWGDT
                     VAAHINKLQARERDPLVINLASQEYFKVTQAKAGRAKVLKARVVECVFEDWKDGDYKI
                     ISFFAKRARGMMARHAIEKRADSADALLDFRGDGYRLAERASEPDRLVFRRRQA"
     gene            complement(5075..6139)
                     /locus_tag="VIN75_08995"
     CDS             complement(5075..6139)
                     /locus_tag="VIN75_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY8974342.1"
                     /translation="MVDRAWVASRKGLIELRRRAGAWAVERTSFVGDPVSMVLPPDAS
                     GRMFAALNLGHFGVKLHASDDAGTSWREVTAPAFGPQPEGAPGPAWKVVMVWSLERQG
                     DALYAGTLPGALFKSTDAAQSWQLDDVLWNQPGRPTWGGGGYDTPGIHSISPLGDDTV
                     LVGVSSAGAWITRDAGATWGQTAHGMRAEYMPPEQAYDPGVQDPHAIARCAAQPDVLW
                     CQHHNGIFRSTNGGARWDELQAPLSSFGFAVAAHPADPQTAWFVPAVKDQCRVPVDRA
                     LVVNRTRDGGASFETLRAGLPQQDCYDLVYRHGLAVADDGTSLLMGSTTGGVWASDDG
                     GDHWTALPARLPPVYAVRFG"
     gene            complement(6167..6436)
                     /locus_tag="VIN75_09000"
     CDS             complement(6167..6436)
                     /locus_tag="VIN75_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8974343.1"
                     /translation="MPHIAFTSQLQRFTETPELHTAAATLRGALDAAFDVNPRLRHYV
                     LDEQGHLRQHVVVYISGRRVDDREALTDALAPDDKVYVLQALTGG"
     gene            complement(6690..8243)
                     /locus_tag="VIN75_09005"
     CDS             complement(6690..8243)
                     /locus_tag="VIN75_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974344.1"
                     /translation="MAMVLACTAVRAGTAEGYEAGRRGDYDTALREFQPAAEKGDRDA
                     QLALADMYLRGYGVAADPAIAARWFRKAAEQGDPRAQTNLGFLYEQGKGVARDFEEAA
                     KWYRKAADQGEPTAQSNLSYMALIGRGVPQDDMQAAHWATLAARQGLAVAQYRLGVFY
                     TNGQGVERDDAQALHWYQAAADQGDARAQSALGWVYSEGRGVPRDLATSLRWLQQSAA
                     QGDPTAEHNLGMALLNGRGLPRDPAGAERWIRKSAEAGYAPAQVSLGLMYAAGDAVVA
                     DEKQAVEWYQKAAAQGNASGQNNLGWMLTNGLGIARDDAAATRQFRLAARKGNAFAMD
                     NLGWMAEEGRGQPADPAEAQRWYRLAEARGNAPARNHLALLYARGKGVARDDAAALGG
                     FRAAAAQGLAPARHNLGYMVANGLGGVPRPDPAAGVALQSLAREASPPSPEELPTLDP
                     ARLSAGERARSAQLLAAFRSAPNVLAVLDAIAGPPSVTSLGPATANPGTTGNGMDGHS
                     VAPPSATQR"
BASE COUNT         1145 a         3030 c         3053 g         1216 t
ORIGIN      
        1 ttgacgaact cggcctggcc gagccgctgc tgcgtgcggt gcacgaagcc ggctacacga
       61 ctcccacccc gatccagctc caagccatcc ccgtcgtcct ccagggcggc gacctcctcg
      121 ccggcgcgca gaccggcacc ggcaagaccg ccggcttcac gctcccgatg ctgcaccgcc
      181 tgctcgaagg cggcccgaag aaggacgccc gcggcaagcc gccggtgcgc gccctgatcc
      241 tgacgcccac gcgcgaactg gccgcccagg tcgaggaaag cgtgcgcacc tacggcaagc
      301 acgtctcgct gtccagcatg gtcatcttcg gcggcgtcgg catgcaaccg cagatcaacc
      361 gcctgcacaa gggcgtcgac atcctggtgg ccacgcccgg ccgcctgctc gacctgcacc
      421 agcagcgcgc ggtcgacctg tcgcacatcg agatcttcgt gctcgacgag gccgatcgca
      481 tgctcgacat tggcttcatc cacgacatca agaaggtgct cgcggtgctg ccccagaaga
      541 agcagagcct gctgttctcg gccaccttca gcgacgagat caaggcgctg gccgaccgcc
      601 tgctgaacaa gccgggcctg atcgaggtcg cgcgccggaa ccagaccaac gagatgatcg
      661 agcaggccgt gcacccggtc tcgcgcgacc gcaagaagca gttgctgtcg cacctgatca
      721 agtcgggcga ctggcaccag gtgctcgtgt tcacgcgcat gaagcacggc gccaaccgcc
      781 tggccgaatt cctgaacgac gacggcatca cggcgatggc catccacggc aacaagagcc
      841 agacggcccg cacccgcgcg ctgtcggagt tcaaggccgg cacgctgcag gtgctggtgg
      901 ccaccgacat cgccgcgcgc ggcatcgaca tcgaccagct gccgcacgtc gtcaactacg
      961 agctgcccaa cgtgccggaa gactacgtgc accgcatcgg ccgcacgggc cgcgccggcg
     1021 cgcagggccg cgcggtgtcg ctggtgtgcg tcgacgagga catcttcctg cgcgacatcg
     1081 agaagctgat caagcgtccg attcccaagg aagtggtgcc cggcttcgag cccgatccgg
     1141 gcgagcgtgc cgagcccatc gtgctgggac gcaccaccat cggcgtgcgc ggcggcccgt
     1201 cctcgggcgg ccgacgcgac ggcggcggcc accatgcgcg cgccggccac ggcgaccggg
     1261 gcggcaatgg ccacggcaac ggcggcggcg atcgcaaccg cggccagggc cgtcccggcg
     1321 gcgcgccggg cgggcgtccg gcgcaacagc gtccgcaggg ctcgtcgggc tcgcatgcgg
     1381 gcgccaagcc ggcgcatggc ggcgcgcatg ccggccacgg cggcgcgcgt ccggccggcg
     1441 gccagggcca tggcaaccac ggaggcggtc gcccggcggg tggcggtggt ggtggccgtc
     1501 cgcaaggcgg cggccagcgc gcggcgctga cctcgccgtc cggcggcaag ggtcccgtca
     1561 agcgctgatc agctcgcctg atccggcaat ccaggcccgc gtgcgcaagc cgcgggcctt
     1621 ttgcatggcg gcgccgaact cgggcgcttc gtggattgct gcacgcacgc caggcgcgcg
     1681 cggatcctgc tcgacggaaa atcgagagct ggccgcacga cggcatcccc gcccccgccc
     1741 acgcgcttgg acctgcacac cctcttcctc gcccagacct gcctgctcgc cgcgacggcc
     1801 gcgatgctgt gggtgtcgcg cagcgacgcc gaccgcggca acggcatgca ggcctggcgc
     1861 ctggcgatca cgagccaggc cgtcgcctat ctcgtgctcg cgatccccgc gtcgggggtg
     1921 gccgcgacgt tcaccggcct ggtcgccaac ctggccgggg cggtgggcgt ggcgctgtat
     1981 ttcatcggcg tgcggcgctt cgcgggcctg cccgtcgacc ggcgcccgct ggcggcgatg
     2041 gtcgcggccg tcacggtggc cggggcggcc acgggcgagc acgtcgcctg ggccgcgatc
     2101 ttcaacggct tcgcctacgc cggctgccag gccttggccg cgctcacgct gtggcgcgcg
     2161 ccgcggtccg gcatggtgcg ggtgcagcag gtggtcgcgg cggcgtacgc cgccatggcg
     2221 gcggtgctgc ccctgcgcgc catcgcgttg ctggcgatgc gcgaatcacg actggatctc
     2281 cgacacgatg cctggcaggt gccgatctac gtgttcgggt tcctctacct tgtcgtcacc
     2341 aacctcggct tcatcctgat gtgcaagatg cgggcggagg ccgagatccg cctgcaggcc
     2401 cgcaccgacg acctgaccgg cctgcccaac cggcgcgcgc tcgacgagga gatcgcccat
     2461 gccctggccg ccgcgcgccg cagcgggcgg cccttcggcg tcgtgatggt ggacgtcgac
     2521 cgcttcaagt tcatcaacga cacccacggc cacgccgtcg gcgacgcgac gctgagcgcg
     2581 ttcgcgcggc gcctggccag cgcgctgcgc gagcccgatc gcgcctatcg ctacggcggc
     2641 gaggagttct gcgtgctgct ggacgacacc gacctcgcgg gcgcccgcac gctcgcggaa
     2701 cgcgcgcgcg agcacatcat gctgcccttc gagggcacga tgagcgcgct gaccgccagc
     2761 ttcggcgcca ccgcctggca gcctgacgac accggcgaca cgctgttccg ccgcgcggac
     2821 cgcgcgctct acgcggccaa gcgcaacggc cgcaaccggg tcgagacggc ctgagacgac
     2881 cgcagcccgc acgagggcgg gctgctggca tcggaacctc gggtcggcgc gttcagacga
     2941 actcgcgctc gcgcagccac ttcgtggcga cccacttctc gccctcgatc accggagcgc
     3001 cgccgtgcag gctcttggtg ttgacgtggg cgcggtcgta gctgaagaag acggcgttgc
     3061 ccttgatcgg ggcgacctcg aagccgacgt cggggaacgt ggtgccgccg cccttcctgg
     3121 gcgtgttcag gtacatcagc acggtgccca cgcgctggcc gccgcgcttg aggatgggcg
     3181 cggagccggg gtgcaccggg tcgaagtagt cgtagtgcgg cttgtactcg gcgcccggct
     3241 ggtagtgcag gacctgcagg ccctcgccgt tctggacggg ccagtccagc agcgccgcga
     3301 tgcgcttttc gaccttgtcg atcacgcccg tctcgccgcg ctcgaagaac atgccgcggc
     3361 tggtgcgcgc gacgttgacc tcgctgccgc cggtcaggtt gtccaccgtc tcggagcgtg
     3421 ccatgcgcgg cttggccagc gcgatgatct ggtcgcactc ggcgtcggac aggaagccgc
     3481 ccagcacgat cacgcgcggc agcttcatga ccgacagcac cgtcacgtcg cggtcgagga
     3541 cgcgcagctt gctgggctgg tcgcggacgg cggcgtcggg cacgggcacc ggctgcggct
     3601 cgtggccctc gggcgcggca gcctgctgcg cctgctggtt ggcgacctgc tggttcaggt
     3661 ggctgcgcag cgtctcctcg agggcctgga tggccacgtc ctcgtcccag ccgctggact
     3721 tcatggcctc cagcacggtt tccggcgcga cgccggcctg tgcctgctgg acgatccatt
     3781 ggcgaagttc caccgagatg tactgctggc gcttgccacc ggtgttctgc gtgctcgatt
     3841 gcgtttgcat gaaatcgact ccgacgtgcg gtcgagggaa aaggcccggg cgccgccgcc
     3901 tgcaggagcg gcggtcttcc ggattcgtat tctgcgtatt ttgacagtgc gccacggtcg
     3961 agtcggcgcg ggaatccctc ggtttggcga tgccgggatg caacttcccg tgaatcccgc
     4021 cggcaaacgt tgcccgggcc ccgcccggcg cgcgattgcg tcagaagctg tgaatgaatc
     4081 cggcgcgccg ctcgggcgtg cggaattcgt tcacggcttc tcaggcctgg cggcgccgga
     4141 agacgaggcg gtcgggttcc gacgcgcgct cggcgaggcg gtagccgtcg ccgcggaagt
     4201 ccagcagcgc gtcggccgaa tcggcccgct tctcgatcgc gtgccgggcc atcatgccgc
     4261 gggcgcgctt ggcaaagaag ctgatgatct tgtagtcgcc gtccttccag tcctcgaaga
     4321 cgcactcgac gacacgcgcc ttcaacacct tggcgcgccc cgccttggcc tgcgtgacct
     4381 tgaaatactc ctgcgacgcg aggttgatca ccagcggatc gcgctcgcgg gcctgcagct
     4441 tgttgatgtg cgcggcgacg gtgtcgcccc accacgcgta cagcgagcgg ccgcgctcgg
     4501 tggccaggcc ggtgcccatc tcgaggcggt acggttgcag ccggtcgagc ggacgcagca
     4561 cgccgtacag gccgctgagc atcagcacgt gctcctgcgc ccacgcgatc tcgtcgccat
     4621 tgagcgtgga agcgcgcagg ccgtcgtaga cgtcgccgtt gaacgcccac atcgcgggcc
     4681 ggctgttgcg ctcggtggcg cgggtcgacc acgccttgta gcgcttgacg ttgagcttgg
     4741 cgaggtcctc ggagaggtcc atcaggtcgg cgatgtcggc caccgtcttg gtgcgcagca
     4801 ccgagatcag ttcggcggcc tgcgccgcgt actgcggagc ggtggcggcg ggcttgagtc
     4861 cggcgcgggc ggcgagcacc ggttcgggca gcggggagtc gtagtcgagg gacttggcgg
     4921 gggacagcaa caggagcatg gccggcagtg tagcgacgcg cgcgaggccg tcgggcgccc
     4981 gcgaaaacga aggcaaaaac gaagggggcc gtcatccgat gcgggccggc gagcccacgg
     5041 ccaggaggtc gagccggatc gcccccgtcg acgctcagcc gaagcgcacc gcgtacaccg
     5101 gcggcaggcg cgcgggcagc gccgtccagt ggtcgccgcc gtcgtcgctc gcccagacgc
     5161 cgccggtggt gctgcccatc aacaggctgg tgccgtcgtc ggccaccgcc aggccgtggc
     5221 gatagacgag gtcgtagcag tcctgctgcg gcagcccggc gcgcagggtc tcgaagctgg
     5281 cgccgccgtc gcgcgtgcgg ttgaccacca gcgcgcggtc gaccggcacg cggcactggt
     5341 ccttgacggc cggcacgaac cacgccgtct ggggatcggc cggatgggcc gccacggcaa
     5401 agccgaacga agacagcggc gcctgcagct cgtcccagcg cgcgccgccg ttggtggatc
     5461 ggaagatgcc gttgtggtgc tggcaccaga gcacgtcggg ctgcgcggcg cagcgcgcga
     5521 tggcgtgcgg atcctgcacg ccggggtcgt aggcctgctc gggcggcatg tactccgcgc
     5581 gcatgccgtg ggcggtctgg ccccaggtgg cgccggcatc gcgcgtgatc caggcgccgg
     5641 cgctgctcac gcccaccagc accgtgtcgt cgcccagcgg gctgatcgag tggatgccgg
     5701 gcgtgtcgta gccgccgccg ccccaggtcg gccgtcccgg ctggttccac agcacgtcgt
     5761 cgagctgcca gctctgcgcc gcgtccgtcg acttgaacag cgcgccgggc aaggtgcccg
     5821 cgtacagcgc gtcgccctgg cgctccaggg accacaccat gacgaccttc cacgccggcc
     5881 ccggagcgcc ttcgggctgc gggccgaagg ccggcgcggt gacctcgcgc cagctcgtgc
     5941 cggcatcgtc ggacgcatgc agcttgacgc cgaagtggcc caggttcagg gccgcgaaca
     6001 tccgccccga tgcatcgggc ggcagcacca tgctcaccgg atcaccgacg aagctcgtgc
     6061 gctccacggc ccacgccccg gcgcgccggc gcagctcgat caagcccttg cgggaggcca
     6121 cccatgcgcg atcaaccatg tccctgctcc ttcgtggatt gccgcttcag ccgccggtga
     6181 gcgcctgcag cacgtagacc ttgtcgtccg gcgcgagcgc gtcggtgagc gcctcgcggt
     6241 cgtccacgcg tcgtccgctg atgtagacca ccacgtgctg ccgcaggtgc ccctgctcgt
     6301 cgagcacgta gtgccgcagc cgcggattga cgtcgaacgc cgcgtccagc gcgccgcgca
     6361 gcgtggccgc ggccgtgtgc agctccggcg tctcggtgaa gcgctgcagt tgcgacgtga
     6421 acgcgatgtg aggcatgtct gcgatggtgt gcgagctcgg cgcggggggg caatcgggcg
     6481 tatcccgtga ttgctggatg gccaggcgtg gcgagacgga acgcgggcga cgcgagcgcg
     6541 cgcaagcttg cgtgatggac ggactgcgcg atgacggcgc cggcatccgc ggcacgggac
     6601 cgacatcggc accgcgtcgc tgacccagag cgacacgccg ggccgtccgc ggcgttgccg
     6661 gtcctgcccc ggatgcggga gaccgcgcct cagcgttgcg tcgccgacgg cggcgcgacg
     6721 ctgtggccgt ccatgccgtt gcctgtcgtg ccggggttgg cggtggccgg gcccagcgag
     6781 gtcacgctcg gcggtccggc gatggcgtcg agcacggcca gcacgttggg cgcgcttcgg
     6841 aaggccgcga gcaattgcgc gctgcgcgca cgctcgccgg ctgacaggcg tgccgggtcg
     6901 agcgtgggca gttcctcggg gctgggcggc gaggcttcgc gcgccaggct ctgcagcgcg
     6961 acgccggcgg ccgggtcggg gcgcggcacg ccgcccaggc cgttggccac catgtagccc
     7021 aggttgtggc gggcgggcgc caggccctgc gcggccgccg cacggaagcc gccgagcgcg
     7081 gccgcgtcgt cgcgcgcgac gcccttgccg cgcgcataca gcagcgcgag gtggttgcgc
     7141 gcgggcgcgt tgccgcgcgc ctcggcgagg cggtaccagc gctgcgcctc cgccggatcg
     7201 gcaggctggc cgcgcccttc ctcggccatc cagcccaggt tgtccatcgc gaaggcgttg
     7261 cccttgcgcg ccgcgagccg gaactggcgc gtggcggcgg cgtcgtcgcg cgcgatgccc
     7321 aggccgttgg tgagcatcca gccgaggttg ttctgccccg acgcgttgcc ctgcgcggcg
     7381 gccttctggt accactcgac ggcctgcttc tcgtcggcca cgaccgcgtc gcccgccgcg
     7441 tacatcaggc ccaggctgac ctgcgcgggc gcgtaccccg cttcggcgga cttgcggatc
     7501 cagcgctccg cgcccgccgg gtcgcgtggc aggccgcggc cgttcaggag cgccatgccg
     7561 aggttgtgtt ccgcggtggg gtcgccctgc gcggccgatt gctgcagcca gcgcagcgag
     7621 gtggcgaggt cacgcggcac gccgcggccc tcgctgtaga cccagccgag cgcgctctgg
     7681 gcgcgcgcgt cgccctggtc ggcggcggcc tggtaccagt gcagcgcctg cgcatcgtcg
     7741 cgctccacgc cctggccgtt cgtgtagaag acgcccagcc ggtactgcgc gacggctagg
     7801 ccctgcctgg cggcgagcgt ggcccagtgc gcggcctgca tgtcgtcctg cggcacgcca
     7861 cggccgatca gggccatgta cgacaggttg ctctgcgcgg tgggttcgcc ctggtcagcg
     7921 gccttgcggt accacttggc ggcctcctcg aagtcgcgcg cgacgccctt gccctgttcg
     7981 tagaggaagc ccaggttggt ctgcgcgcgc gggtcgccct gctcggcagc cttgcgaaac
     8041 cagcgcgcgg cgatcgccgg gtcggccgcg acgccatacc cgcgcagata catgtcggcc
     8101 agcgccagct gcgcatcgcg atcgcccttc tcggccgctg gctggaactc gcgcagcgcg
     8161 gtgtcgtagt cgccacggcg tccggcctcg tagccctcgg ccgtgccggc gcgcaccgcc
     8221 gtgcaggcga gcaccatggc catggcaacg agcgaacacg cgatcagcga acgcggcgcc
     8281 tcaccccgag acaacacgca caccatgcca gacgccgacc gattcgagcg gcgcggcagg
     8341 cgcaccgaga gcgcggaggc gtggcaggca ggcaatggat tcatttcgag cggaagtcct
     8401 cgagggatgt tagcgcgaag gatccggggg cgggggccga gtcg
//
LOCUS       DATCRO010000333         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_566507, whole genome shotgun sequence.
ACCESSION   DATCRO010000333 DATCRO010000000
VERSION     DATCRO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566507"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VIN75_09010"
     CDS             <1..901
                     /locus_tag="VIN75_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8974345.1"
                     /translation="VNQAAAALGYRPNHAARVLRSRLSRTVGCLLPDISNPLYAQVFR
                     ALEDEFLRHDYLLLLANGANDVDRETKALGTFTQRGMDGVILAPGNERNAALVEALRV
                     LPMPAVVFDREVSGAHDAVLIDHAGGVRRVVEHLLGLGHRDIALVLWQGDTRPVRRRV
                     QGFRTAFQAAGLPVPDLLVQARSVTASVKAEVTELLARPERPTALIVQGTHMLNSALR
                     AIAARGLQVPRDISVVAIGDTPFAAEHDPAVSVLDIDQSETARCLVDLLLTRIADNGL
                     PQRKARVGLRYVERDSVARPASA"
     gene            complement(898..1857)
                     /locus_tag="VIN75_09015"
     CDS             complement(898..1857)
                     /locus_tag="VIN75_09015"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8974346.1"
                     /translation="MTHALPFLDRLALPLIAAPMLRVSGIELVSAACAAGVIGAFPTA
                     NCQTLDEFDAWLTRLDEEAAAAPERTAPCCPNLIMRRDPARVMAEVELIARHRAQLVI
                     TSVGSPASVVPRLHEASCRVFADVASLHHAVRAIESGVDGLVLLTAGAGGHTGWANPF
                     AFVRAVRRVFDGPIVLAGGLSDGVALRAARELGCDLGIMGTRFIATQESLASDDYRHM
                     LVDSSIDDVLLTRGFTGLPASFLAPSARRLGIDISTLDESISVEEARRRYGGGANAAP
                     NQRWTDIWSAGHSVSGVRAVPTVAELVDALVEEYRGTAAADRS"
     gene            complement(1854..2858)
                     /locus_tag="VIN75_09020"
     CDS             complement(1854..2858)
                     /locus_tag="VIN75_09020"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8974347.1"
                     /translation="MSQPPLSTRLTRLFGLAYPILGGGLMWLSDAHYVGALTNAGCMG
                     FITPRSFDGEAALREQLRRCSEITGGRPFGVNLTLSARPQANAGVQASLETALAHGVR
                     HFETAGYAPDALIRAIHAAGGIVIHKASSVRHALAAQAAGADAVALVGMEEGGHPGAN
                     ELPTMLMGALAMARGQFHVPLVLGGGIGHGSQLAAALALGADGVLMGSRFLVSNEVSA
                     HAAYKNHLVDCDEHSTMRLLHTLGNTWRVLRNDTAAQVDTIERAGAADHAAFGALLDS
                     RVARDRCYGDGDWRNGMLSLGPAIGFATRTEPLAAIVEQLVLQTRAALAHLNASTALP
                     "
     gene            complement(2855..>3150)
                     /locus_tag="VIN75_09025"
     CDS             complement(2855..>3150)
                     /locus_tag="VIN75_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8974348.1"
                     /translation="TLGARKKQAQAINDRIAQQLAARDSAEAFETMARNDLPVAPVVP
                     PAELAALPQFAQRGLVNGTEALSLVRFPVPLEGVDPSALGDAPGLDNARQLLP"
BASE COUNT          443 a         1093 c         1182 g          432 t
ORIGIN      
        1 ggtcaaccag gcggcggcgg cattgggcta ccgccccaac catgcggcgc gcgtgctgcg
       61 ttcgcggctc agccgcaccg tcggctgcct gctgcccgac atctcgaacc cgctgtatgc
      121 gcaggtgttc cgcgcgctgg aggacgagtt cctgcggcac gactacctgc tgctgctggc
      181 caacggcgcc aacgacgtcg accgcgagac gaaggcactg ggaacgttca cgcagcgcgg
      241 catggacggc gtgatcctcg cgcccggcaa cgagcggaat gcggcgctgg tggaggcgct
      301 gcgggtgctg ccgatgccgg ccgtggtgtt cgaccgcgaa gtcagcggcg cccacgatgc
      361 cgtgctcatc gatcacgcgg gcggggtgcg gcgcgtggtc gagcacctgc tcggcctggg
      421 gcaccgcgac atcgccctcg tgctctggca gggcgatacg cgcccagtgc gccgccgcgt
      481 gcagggcttc cgcacggcgt tccaggccgc gggcctgccc gtgcccgatc tgctggtgca
      541 ggcgcgcagc gtgacggcct cggtcaaggc ggaggtcacc gaactgcttg cgcgaccgga
      601 gcgacccacc gcactcatcg tgcagggcac ccacatgctc aacagcgcac tgcgcgcgat
      661 tgctgcgcgc gggctccagg tgccgcgcga catctcggtg gtcgccatcg gcgatacgcc
      721 cttcgccgcg gagcacgacc ccgcggtgag cgtgctcgac atcgaccagt ccgagaccgc
      781 gcgctgcctg gtcgacctgc tgctcacgcg gatcgccgac aacggcctgc cgcagcgcaa
      841 ggcgcgcgtc ggcctgcgct atgtggagcg cgattcggtg gccaggcccg cgtcggccta
      901 ggaccgatca gccgccgcag tgccacggta ttcctcaacg agcgcatcca ccagctcggc
      961 gaccgtcggc acggcgcgca cgccggacac tgaatgcccc gcgctccaga tgtcggtcca
     1021 gcgctggttg ggcgccgcat tggcgccgcc gccgtagcgt cttctcgctt cctcgaccga
     1081 gatgctctcg tcgagcgtcg agatgtcgat gcccagccgg cgcgccgacg gcgcaagaaa
     1141 gctggcgggc agcccggtga agccgcgcgt gagaagcacg tcgtcgatcg agctgtcgac
     1201 cagcatgtgc cggtagtcgt cgctcgccag gctttcctgc gtggcgatga agcgcgtgcc
     1261 catgatgccg aggtcgcagc ccagttccct ggcggcgcgc agggccacgc cgtccgacag
     1321 cccgcccgcc agcacgatgg ggccgtcgaa cacgcggcgc accgcgcgca cgaaggcgaa
     1381 cgggttcgcc cagccggtgt ggccgccggc gcccgcggtg agcagcacga ggccatcgac
     1441 gcccgactcg atggcgcgca ccgcatggtg cagcgaggcc acgtcggcga acacgcggca
     1501 gctggcttcg tgcaggcgcg gcaccactga ggccggcgag cccacgctgg tgatcacgag
     1561 ctgggcgcgg tgccgggcga tgagttcgac ctcggccatc accctggcgg ggtcgcgccg
     1621 catgatgagg ttggggcagc agggcgccgt gcgctcgggc gcggcggcgg cttcttcatc
     1681 caggcgggtg agccaggcgt cgaattcgtc gagcgtctgg cagttggccg tcggaaaggc
     1741 gccgatcacg ccggcagcgc aggcggcgga caccagttcg atgccagaga cacgcagcat
     1801 gggcgcggcg atcagcggca gagccagtcg gtcgaggaag ggaagggcgt gggtcatggc
     1861 agcgcagtgg atgcgttcag gtgcgccagg gcagcgcgcg tttgcaggac gagttgctcg
     1921 acgatcgcgg cgagcggctc ggtgcgcgtg gcgaagccga tggccgggcc gagcgagagc
     1981 atgccgttgc gccagtcgcc gtcgccgtag cagcggtcgc gcgccacgcg gctgtcgagc
     2041 agggcgccga aggcggcgtg gtcggccgcg ccggcgcgct cgatggtgtc gacctgcgcg
     2101 gcggtgtcgt tgcgcagcac gcgccaggtg ttgcccagcg tgtgcagcag gcgcatggtg
     2161 ctgtgctcgt cgcagtccac caggtggttc ttgtaggccg catgggccga gacctcgttg
     2221 ctcacgagga agcggctgcc catcagcacg ccgtcggcac cgagcgcgag tgcggcggcg
     2281 agctggctgc cgtggccgat gccgccgccc agcaccagcg gaacgtggaa ctgcccccgc
     2341 gccatggcca gcgcgcccat cagcatggtc ggaagctcgt tggcgccggg gtgcccgcct
     2401 tcctccatgc cgaccagcgc gaccgcgtct gcgccggccg cctgggcggc gagggcatgc
     2461 cgcacgctcg atgccttgtg gatcacgatg ccgccggctg cgtggatggc ccggatcagc
     2521 gcatcgggcg cgtagcccgc ggtctcgaag tggcgcacgc cgtgcgccag cgcggtctcc
     2581 agcgaagcct gcacgccggc attggcctgc gggcgtgccg acagcgtgag gttgacgccg
     2641 aagggacggc ctccggtgat ctcgctgcag cgccgcagct gctcgcgcag cgcggcctcg
     2701 ccgtcgaagg agcgcggggt gatgaagccc atgcacccgg cgttggtcag cgcgcccaca
     2761 tagtgcgcgt ccgagagcca catcagcccg ccgccgagga tcgggtaggc caggccgaag
     2821 agacgcgtca ggcgggtgga cagcggaggc tggctcacgg cagcagctgg cgtgcgttgt
     2881 cgaggccggg cgcatcgccc agcgccgacg gatcgacgcc ctcgagcggc accggaaagc
     2941 gcaccagcga cagcgcttcg gtgccgttga ccaggccgcg ctgcgcgaac tgcggcagcg
     3001 cagcgagctc ggcgggcggc accacgggcg cgacgggcag gtcgttgcgc gccatcgtct
     3061 cgaaggcttc ggcggagtcg cgcgcggcga gctgctgcgc gatgcggtcg ttgatggcct
     3121 gtgcctgctt cttgcgcgcg cccagcgtgc
//
LOCUS       DATCRO010000334         6738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_93408, whole genome shotgun sequence.
ACCESSION   DATCRO010000334 DATCRO010000000
VERSION     DATCRO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93408"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /gene="earP"
                     /locus_tag="VIN75_09030"
     CDS             complement(<1..164)
                     /gene="earP"
                     /locus_tag="VIN75_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEY8974349.1"
                     /translation="MVPATTSPSPAPPLRWDIFCRVIDNFGDIGVCWRLACNLAERGQ
                     RVRLWVDDPSA"
     gene            261..2597
                     /gene="uvrC"
                     /locus_tag="VIN75_09035"
     CDS             261..2597
                     /gene="uvrC"
                     /locus_tag="VIN75_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8974350.1"
                     /translation="MPRPKSPKSAPADAGAPIPAAVAKKGGPTAFPVSSSQLGSVEDV
                     EAWIGNAAQEDTRASKAEAADRAAVDAAREARERVLAEVAALPAMPGVYRYFDVAGTV
                     LYVGKARNLRRRVASYFQKDHGGTRIGHMIGRIARMETTVVRTEAEALLLENNLIKTL
                     NPKFNILFRDDKSYPYLRLVTHAYPRVAYYRGAVDRKHKYFGPYPGAWAVKETIQLIQ
                     KVFRLRTCEDTVFANRSRPCLLYQIQRCSGPCVGLVSPADYARDVNDAERFLLGDQET
                     VMAELQSQMLRHAEVLEFERAAEFRNRISALSRVLHQQAMDESSLSARDKDVDILAVK
                     VQGGRACVNLAMVRGGRHLGDRAYFPTHVEDATAILGGIESDLGEDDDAPVQVAEPAV
                     QVLEAFIAQHYLGSAPPPQWVTSHPVSKSLVEALSQQTGVKISVQHNPREQRRIWLEM
                     CEKGADLALGRLLAEEGSQRERTRALADAIDLAPDDLDTLRIECFDISHTAGEATQAS
                     CVVYEGHKMQGAQYRRFNITGIVGGDDYAAMRQVLTRRYSRFGVAPAAASADVQPASA
                     DEASADAAQQVSQDVAADAQSDPLTGAPLAAESAGSEEPLVDAALAEPAAGADARPGK
                     KSSSARLPDLVLVDGGRGQVAMAREVFQSLGLDISRIVGVEKGEGRKVGLEELVFADG
                     RDKLALPPDSAALMLVAQIRDEAHRFAITGMRAKRASVRTGGSKLEEIPGIGAKKRAK
                     LLQRFGGVRGVVAASVEELMAVDGISRTLAEEIYRALH"
     gene            2584..3108
                     /locus_tag="VIN75_09040"
     CDS             2584..3108
                     /locus_tag="VIN75_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974351.1"
                     /translation="MPCTDQRDGAPHAPGRLRRVAAWLGLCALSACQTPAPAPQPARA
                     PSPAPAARVTPAPAPAPAPYARPARSAEEYKRQIALRLVAANPKITYLTRAPDILLAI
                     PILEVEVNADGSVRHISVVRTPTQATDTVQIAIDAVRRSAPFGDASQVPRPWKFMQVF
                     LFNDDRHFKPKDLD"
     gene            3166..3732
                     /gene="pgsA"
                     /locus_tag="VIN75_09045"
     CDS             3166..3732
                     /gene="pgsA"
                     /locus_tag="VIN75_09045"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8974352.1"
                     /translation="MFFTIPTLLTWARIVAIPLIVGVFYLPIADPMRNLIATVLFVAT
                     ALTDWLDGYLARKLNQTSAFGAFLDPVADKFLVCASLLVLVHLQRCNALLALVIIGRE
                     IAISSLREWMAQIGASKSVAVHMLGKWKTTMQMVAIPFLLFDGSLFGLIDTHLWGTWL
                     LVIAAVLTIWSMVYYLQKAIPEIRAKAR"
     gene            3977..4249
                     /locus_tag="VIN75_09050"
     CDS             3977..4249
                     /locus_tag="VIN75_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8974353.1"
                     /translation="MNKSELIEHIARQADISKAAATRALEAVIGGVKTTLKKGGSVSI
                     VGFGTFAVTKRAARVGRNPRTKEPIKIKSAKVPKFRPGKGLKDAVN"
     gene            4305..4380
                     /locus_tag="VIN75_09055"
     tRNA            4305..4380
                     /locus_tag="VIN75_09055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4338..4340,aa:Val,seq:tac)
     gene            4490..6409
                     /locus_tag="VIN75_09060"
     CDS             4490..6409
                     /locus_tag="VIN75_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY8974354.1"
                     /translation="MFDFVRSHNRLLQVALGLLIIPSFAIFGVSSYTSSNNEASQAVA
                     SVDGRDISRAEWDAQHRKDVELIRQRAPQVDLKLLDTPEAQRRSLDTIVRERVTTAAI
                     DHEHLAVSEDRALHEYQTNPEFAPQRAMSKQERDAWLADRRLTSGQLLANIAQSMQRQ
                     EATRGAADSGFMPNTTLKVSTDAWFDQREIQWQHFDPKDYAAKIQPTDAQVDAYYKAH
                     SADFFAPEQARIEYVVLDPAALTSQVKADPTLVQEYYKNNAARYTTPEERSASYILSK
                     VAPNASPADVAKAKALAETELAEVRKNPAAFSEIARKIPQDGGPLEGGDLDFMRKGAL
                     GIPALDDALFKMKDGEISDIIRIDSGFQIVKVTGVRGGAVKTFEQVKGEIEDLLKQQE
                     AQKLFTSDAEKFSNMVYEQPDSLDPAAKAFSLTKQTATVTRTPAADAQGPLASPKLLS
                     AIFASDAIKARHNTEAIEAGQSQLVSAHVVEYLPQRTRPLAEVHDQVVDALRKAEAAA
                     AAKRDGEARVAEAKKDPSLALPQAATVGRMDRGGAVPVQVMQAALKADLSKGPTVVGV
                     ALPDGGYAAIRVVKSTPHVPNANESAQWKTVATQAYEDSVAEAIYDSQKLRYKVKYNE
                     ERIAKVTSQAASAPN"
     gene            6440..6515
                     /locus_tag="VIN75_09065"
     tRNA            6440..6515
                     /locus_tag="VIN75_09065"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6473..6475,aa:His,seq:gtg)
BASE COUNT         1163 a         2348 c         2249 g          978 t
ORIGIN      
        1 gcgctcggat cgtccaccca tagccgcacg cgctgcccgc gctcggccag gttgcaggca
       61 agccgccagc acacgccgat gtcgccaaag ttgtcgatga cgcggcagaa gatgtcccac
      121 cgcaggggcg gcgccgggga aggcgaagtc gtcgcgggaa ccatggaggg cgtcaggtct
      181 tggagcgttg cggtggcagg ttgcgaatct tgcaccacgt cccgtgggac gtcacgacgg
      241 cgcggcacaa tgacgagtcc atgcctcgtc ccaagtcccc gaagtccgcg ccggccgatg
      301 ccggtgcgcc gatcccggcc gccgtcgcga agaagggcgg ccccacggcg tttccggtgt
      361 cgtcgtcgca gctgggcagc gtcgaggacg tcgaggcgtg gatcgggaat gcggcgcagg
      421 aagacacccg cgccagcaag gccgaggccg ccgaccgtgc cgccgtcgac gccgcgcgcg
      481 aggcgcgcga gcgcgtgctg gccgaggtcg cggcgctgcc cgcgatgccc ggcgtctacc
      541 gctacttcga cgttgccggc accgtgctgt acgtgggcaa ggcgcgcaac ctcaggcgcc
      601 gggtcgccag ctacttccag aaggatcacg gcggcacgcg catcggccac atgatcggcc
      661 ggatcgcgcg gatggagacc acggtcgtgc gcaccgaggc cgaggcgctg ctgctcgaga
      721 acaacctgat caagacgttg aacccgaagt tcaacatcct gttccgcgac gacaagagct
      781 acccgtacct gcgactggtc acgcacgcct atccgcgcgt ggcgtactac cgcggggcgg
      841 tcgaccgcaa gcacaagtac ttcgggccgt atccgggcgc gtgggccgtc aaggagacga
      901 tccagctgat ccagaaggtg ttccgcctgc gcacctgcga ggacaccgtg ttcgccaacc
      961 gctcgcggcc ctgcctgctg taccagatcc agcgctgctc cgggccgtgc gtgggcctgg
     1021 tctcgccggc cgactacgcg cgcgacgtca acgacgccga gcgcttcctg ctgggcgacc
     1081 aagagacggt gatggccgag ctgcagtcgc agatgctgcg ccacgccgag gtgctcgagt
     1141 tcgagcgcgc ggcggaattc cgcaaccgca tctcggcgct gtcgcgcgtg ctgcaccagc
     1201 aggcgatgga cgagagcagc ctgtccgcgc gcgacaagga cgtcgacatc ctggccgtga
     1261 aagtgcaggg cggccgggcc tgcgtcaacc tggcgatggt gcgcggcggc cgccacctcg
     1321 gcgaccgcgc gtatttcccg acgcacgtgg aggacgccac cgcgatcctc ggcggaatcg
     1381 agtccgacct cggcgaggac gacgacgcgc ccgtccaggt cgccgaaccc gcggtgcagg
     1441 tgctggaggc gttcatcgcg cagcactacc tgggcagcgc gccgccgccg caatgggtca
     1501 ccagccatcc ggtgagcaag tcgctggtcg aggcgctgtc gcagcagacc ggcgtgaaga
     1561 tcagtgttca gcacaatccg cgcgagcagc gccgcatctg gctcgagatg tgcgagaagg
     1621 gggccgacct cgcgctgggc cgcctgctgg ccgaggaagg ctcgcagcgc gagcgcaccc
     1681 gcgcgctggc cgacgcgatc gacctcgcgc ccgacgacct cgacacgctg cgcatcgagt
     1741 gcttcgacat cagccacacc gccggcgagg cgacgcaggc ctcgtgcgtc gtctacgagg
     1801 gccacaagat gcagggcgcc caataccggc gcttcaacat caccggcatc gtcggcggcg
     1861 acgactacgc cgcgatgcgg caggtgctca cgcgccgcta ttcgcgcttc ggcgtggcgc
     1921 cggcggcagc gtcggccgac gtgcagccgg cctccgcgga cgaggcatcg gccgacgccg
     1981 cgcaacaggt gtcgcaggat gtcgcggccg acgcgcagtc cgacccgctg accggcgcgc
     2041 cgctggccgc cgaatcggcc ggcagcgagg agccgctggt ggacgccgcg ctcgccgaac
     2101 cggccgccgg ggcggacgcc aggcccggga agaagtcctc cagcgcgcga cttccggatc
     2161 tcgtgctggt ggatggcggc cgcggccagg tcgcgatggc gcgcgaggtc ttccagtcgc
     2221 tgggcctgga catctcgcgc atcgtcggcg tcgagaaggg cgaggggcgc aaggtcggcc
     2281 tcgaggagct cgtcttcgcc gacggccgcg acaagctcgc gctgccgccc gattccgccg
     2341 cgctgatgct ggtggcgcag atccgcgacg aggcacaccg cttcgcgatc accggcatgc
     2401 gcgccaagcg cgcgagcgtg cgcacggggg gcagcaagct ggaggaaatc cctggcatcg
     2461 gcgccaagaa gcgcgcaaag ttgttacaac gttttggcgg cgtccgcggc gtcgtggccg
     2521 ccagcgttga agaattgatg gccgtcgacg gcatctcgcg aaccctggcc gaggagatct
     2581 accgtgccct gcactgacca acgagacggc gcgccgcacg cgcccggacg gctgcgccgc
     2641 gtcgccgcct ggctggggct gtgcgcgctg tccgcctgcc agacgccggc gcccgcgccg
     2701 cagcccgcgc gcgccccgtc gcccgcgccc gccgcgcgcg tgacgcccgc ccccgcgccg
     2761 gcgcccgcgc cctacgcgcg ccccgcgcgc tcggccgagg agtacaagcg ccagatcgcg
     2821 ctgcgcctgg tcgccgccaa ccccaagatc acctacctca cccgcgcgcc ggacatcctg
     2881 ctggcgatcc cgatcctgga ggtcgaggtc aacgccgacg gcagcgtgcg ccacatcagc
     2941 gtcgtgcgca ctcccacgca ggccaccgac acggtgcaga tcgcgatcga cgccgtccgc
     3001 cgctcggcgc cgttcggcga cgcgtcgcag gtgcccaggc cctggaagtt catgcaggtg
     3061 ttcctgttca atgacgaccg gcacttcaag cccaaggatc tcgactgaag cgagattgcg
     3121 gacacccgca gccgcgcgcc acgcattgca aggcagaatg ccccgatgtt cttcacgatc
     3181 ccgactctgc tgacctgggc gcgcatcgtc gcgatcccgc tgatcgtcgg cgtgttctac
     3241 ctgccgatcg ccgatccgat gcgcaacctg atcgccacgg tgctgttcgt ggcgaccgcg
     3301 ctgaccgact ggctcgacgg ctacctcgcg cgcaagctga accagacctc ggccttcggc
     3361 gcgttcctcg atccggtggc cgacaagttc ctcgtgtgcg cgtcgctgct ggtgctggtg
     3421 cacctgcagc gctgcaacgc gctgctggcg ctggtcatca tcggccgcga gatcgcgatc
     3481 tcgtcgctgc gcgaatggat ggcccagatc ggcgcgtcca agagcgtggc ggtgcacatg
     3541 ctcggcaagt ggaagacgac catgcagatg gtggccatcc cgttcctgct gttcgacggc
     3601 agcctgttcg ggctgatcga cacgcacctg tggggcacct ggctgctcgt cattgctgcg
     3661 gtgctgacga tctggtcgat ggtctattac ctgcagaagg ccatcccgga gatccgtgcg
     3721 aaagccaggt gagcgagccc tggcgatcag ggtttgcgct gatcgctccc gcggccgctg
     3781 cgcaagcttg tgtaacgaat cgccatacaa tccgcctctc gcttgacact gtcaggccac
     3841 aaggtctgta atcatccgtg gccgccgagg cctcgatttt tcaaggcggg tcgccatgca
     3901 gatggcggtc cgccgcggtc aggaacgacc gctccatcaa acaatcgcca gcgtatttcc
     3961 gagagggggt tccttcgtga acaagtctga attgatcgaa cacatcgccc gccaggccga
     4021 catctccaag gccgcggcaa cccgcgcgct ggaggcggtc atcggcggcg tgaagaccac
     4081 cttgaagaag ggtggcagcg tgtcgatcgt cgggttcggc accttcgccg tgaccaagcg
     4141 cgccgcgcgc gtgggccgca atccgcgcac caaggaaccc atcaagatca agtcggccaa
     4201 ggtgccgaag ttccgtccgg gcaagggcct gaaggacgcg gtcaactgac acccgggtgc
     4261 cgcgccgccc gcaccgaggc ggcgcacggc accctggctc cagcgggtgc ttagctcagt
     4321 tggtagagca gcgcccttac acggcgtagg tcggcggttc gagcccgtca gcacccacca
     4381 cccctcgatc gattccctgt tccggtcgaa ccaggcgaac tccggcaacc gagttcgcct
     4441 ttgtcgtttt ttgcgatagc gatcctcgtt ccgttccaag aggctttcca tgtttgattt
     4501 cgtccggtcg cacaaccgac tcctgcaggt cgccctcggc ctgctgatca tcccgtcgtt
     4561 cgcgatcttc ggtgtgtcga gctacacgtc gagcaacaac gaggcgtcgc aggccgtggc
     4621 ctccgtcgac ggccgcgaca tctcgcgcgc ggagtgggat gcccagcatc gcaaggacgt
     4681 cgagctgatc cgccagcgcg cgccgcaggt cgacctgaag ctgctggaca cgcccgaggc
     4741 gcagcgccgc tcgctcgaca cgatcgtgcg cgaacgcgtg acgacggccg ccatcgacca
     4801 cgagcacctg gccgtgagcg aggatcgcgc gctgcacgag taccagacga atcccgagtt
     4861 cgcgccgcag cgcgcgatgt ccaagcagga acgtgacgca tggctggccg accgccgcct
     4921 caccagcggc cagctgctgg ccaacatcgc ccagtcgatg cagcgccagg aggccacgcg
     4981 cggcgcggcc gacagcggct tcatgccgaa caccacgctc aaggtcagca ccgatgcctg
     5041 gttcgaccag cgcgagatcc agtggcagca cttcgatccg aaggactacg ccgccaagat
     5101 ccagcccacc gacgcgcagg tcgacgccta ctacaaggcg cactcggccg acttcttcgc
     5161 gcccgagcag gccaggatcg aatacgtcgt gctggatccg gcggcgctga cgtcgcaggt
     5221 caaggccgat ccgacgctgg tgcaggagta ctacaagaac aacgcggccc gctacacgac
     5281 gcccgaggag cgcagcgcca gctacatcct gtccaaggtg gcgcccaacg cgtcgcccgc
     5341 cgacgtcgcc aaggccaagg ccctggccga gaccgagctg gccgaggtgc gcaagaaccc
     5401 ggccgcgttc tccgagatcg ccaggaagat cccgcaggac ggcggcccgc tcgaaggcgg
     5461 cgacctcgac ttcatgcgca agggcgcgct gggcatcccg gcgctcgacg acgcgctgtt
     5521 caagatgaag gacggcgaga tcagcgacat catccgcatc gacagcggct tccagatcgt
     5581 caaggtcacc ggcgtgcgcg gcggtgcggt caagacgttc gagcaggtca agggcgagat
     5641 cgaggacctg ctcaagcagc aggaggcgca gaagctgttc acgagcgacg ccgagaagtt
     5701 cagcaacatg gtctacgaac agcccgacag cctcgatccg gcggccaagg cgttctcgct
     5761 gaccaagcag accgccacgg tcacgcgcac gcccgcggcc gacgcgcaag gcccgctggc
     5821 ctcgcccaag ctgctgtcgg ccatcttcgc gtccgatgcc atcaaggcca ggcacaacac
     5881 cgaggccatc gaggccggcc agagccagct cgtgtccgcg cacgtcgtgg agtacctgcc
     5941 gcagcgcacg cgtccgctgg ccgaggtgca cgaccaggtc gtcgacgcgc tgcgcaaggc
     6001 cgaggccgcc gcggccgcga agagggacgg cgaggcccgc gtcgccgagg ccaagaagga
     6061 cccgtcgctg gcgctgccgc aggcggccac cgtcggccgc atggaccgcg gcggcgccgt
     6121 gccggtgcag gtgatgcagg ccgcgctgaa ggccgacctg tccaagggcc cgaccgtggt
     6181 cggcgtcgcg ctgcccgacg gcggctacgc ggcgatccgc gtcgtgaagt ccacgccgca
     6241 cgtgcccaac gccaacgaat cggcgcagtg gaagaccgtg gccacccagg cctacgagga
     6301 ttccgtggcg gaggccatct acgactcgca gaagctgcgc tacaaggtca agtacaacga
     6361 agagcgtatc gccaaggtca cgtcgcaagc ggcatctgcc ccgaactgaa aaagcgcgct
     6421 ataatcattg gctccggtgg tggctatagc tcagttggta gagtcccaga ttgtgattct
     6481 ggttgtcgtg ggttcgagtc ccattagcca ccccaaaaaa cacgaggcct cgtaagtact
     6541 tgatcttcaa ggactttcgg ggccttcctg tttctgcatg ggtacagcgt aggggtacgt
     6601 cacgggtaca gtagcatcgc ttgcccggag cataagagtc gagtcgcttg aatgcctact
     6661 cccgaacgcc tcatcgaaaa gctgaacgcc ctagtcgctg aggctgacaa catcaataag
     6721 cgctccatcc tgagggtg
//
LOCUS       DATCRO010000335         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_82431, whole genome shotgun sequence.
ACCESSION   DATCRO010000335 DATCRO010000000
VERSION     DATCRO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82431"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VIN75_09070"
     CDS             complement(<1..1142)
                     /locus_tag="VIN75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8974355.1"
                     /translation="MLSDDDLSALVRARHPDPFAVLGMHADPKGRLWVHAFLPHAVAV
                     EVLDAEGEFVARLDRRTGGDVFEGQVAGRKERFDYRLRVRWDSGVDGVYADPYAYGPL
                     IADDDLHFLAEGKHLRPYEVLGALPMTLGEGAHAVAGVRFAVWAPNARRVSVVGDFNN
                     WDGRRHPMRSRGGSGVWEIFVPHLVEGDRYKYEIVGPDDALQPLKADPYARAAQLRPD
                     SASLVAPMPPRRELPASRAGMNDRHAPVSIYEVHLGSWRRHVNGGFKTWDELAAELPP
                     YVADLGFTHVELMPITEFPFDGSWGYQTLGLFAPTARFGPPEGFARFVDACHAKGIGV
                     ILDWVPAHFPVDAHGLARFDGTALYEYEDPREGFHRDWNTYIYNFGR"
     gene            complement(1172..3577)
                     /locus_tag="VIN75_09075"
     CDS             complement(1172..3577)
                     /locus_tag="VIN75_09075"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY8974356.1"
                     /translation="MTQEFTTAWKRELSQRSDAPSPDACLRAAVVACRPLLADRWAAT
                     QAADAKRPRGTRRVHYLSMEFLMGRALRNALSVMGLEGAMQAALDSRSGAASLAEVLE
                     QEPDAALGNGGLGRLAACFLDSFATLELPSFGYGLRYAHGMFAQAIQEGRQVELPDEW
                     LRHGNPWEFVRPELRYPVGFGGVVESSGGRRAWKPAEEVVAQACDFVVPGHATERVST
                     LRQWHAQPSRPIDFAMFCRGEHLAAGQHYEAADTLNWVLYPDDSTAAGRQLRLKQEFF
                     LCSASLQDMIARHLREHGEIDTFGRRNAVHLNDTPPALAPAELMRILLDEFHMGWDEA
                     WAVTTQAVSYTNHTLMPEALETWPVRMFEELLPRHLEIVYEINRRFLDEVGTRFPGDT
                     GLIARLSLIDENGERRVRMASLAIVASHKVNGVSALHSRLMVETIFADFAAMFPQRFL
                     NVTNGVTPRRWLMQANPGLSGLLDRHIGNEWRANLDGLKALAAKAGNRKIGEEFLQVK
                     RANKERLAERVRRDFGVTISPDSLFDVQIKRIHEYKRQLLNVLHVVARYQAIVANPGA
                     DWVPRTVIFAGKAASAYHAAKLTIQLIHDVARVVNADPRVGNKLKVVFLPNYGVSLAE
                     VILPAADLSEQISTAGTEASGTGNMKFALNGALTIGTWDGANIEMAEAMDVENMFVFG
                     LRTDAVAKMKSWGYDPRLYAEENQALQQVLDAIGGGVFSWGEPDRYRSLVQPLLHRDP
                     YMLLADFADYVHAQEQVDALYRQPQAWAERALRNIAGMGAFSTDRTIAEYIQHVWAEP
                     KGR"
     gene            3969..>4258
                     /locus_tag="VIN75_09080"
     CDS             3969..>4258
                     /locus_tag="VIN75_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974357.1"
                     /translation="MAHVQLVKVQKAFGDIQILRDIDLEIRDGEFMVFVGPSGSGKST
                     LLRTIAGLEDITGGTLSIGGKVVNDLPPADRGIAMVFQSYALYPHMNLYDNMA"
BASE COUNT          656 a         1439 c         1505 g          658 t
ORIGIN      
        1 cggccgaagt tgtagatgta ggtgttccag tcgcggtgga agccctcgcg cggatcctcg
       61 tactcgtaca gcgccgtgcc gtcgaagcgc gcgaggccgt gggcatcgac cgggaaatgc
      121 gccggcaccc agtccaggat cacgccgatg cccttggcgt ggcaggcgtc gacgaaacgc
      181 gcgaagcctt cgggcgggcc gaagcgcgcg gtgggcgcga acaggcccag cgtctggtag
      241 ccccaggagc cgtcgaacgg gaactcggtg atgggcatca gctccacgtg cgtgaagccc
      301 aggtcggcga cgtagggcgg cagttcggcc gccagctcgt cccaggtctt gaagccgccg
      361 ttgacgtggc gccgccacga gcccaggtgc acctcgtaga tggacaccgg cgcatggcgg
      421 tcgttcatgc cggcgcgcga ggccggcagc tcgcgccgcg gcggcatcgg ggccaccagg
      481 ctggcgctgt ccgggcgcag ttgcgcggcg cgcgcgtacg ggtcggcctt gagcggctgc
      541 agcgcgtcgt cggggccgac gatctcgtac ttgtagcggt cgccctcgac caggtgcggg
      601 acgaagatct cccacacgcc cgagccgccg cgcgagcgca tcggatggcg acggccgtcc
      661 cagttgttga agtcgcccac cacgctgacg cggcgcgcgt tgggcgccca cacggcaaag
      721 cgcacgccgg cgaccgcgtg cgcgccctcc cccagcgtca tgggcagcgc gcccagcacc
      781 tcgtagggcc gcaggtgctt gccctcggcc aggaaatgca ggtcgtcgtc ggcgatcagc
      841 gggccatacg catacgggtc ggcgtacacg ccgtcgacgc ctgaatccca gcgcacgcgc
      901 aggcggtagt cgaagcgctc cttgcggccc gcgacctggc cctcgaacac gtcgccgccg
      961 gtgcggcgat cgagcctggc gacgaactcg ccctcggcgt ccagcacctc gacggccacg
     1021 gcgtgcggca ggaacgcgtg cacccagagc cggcccttcg gatcggcgtg catgcccagc
     1081 accgcgaacg ggtcggggtg gcgcgcgcgc acgagcgcgc tcaggtcgtc atcggacagc
     1141 atgggcgact gctccgcggc aggcatggcg atcaacggcc cttgggttcc gcccacacgt
     1201 gctggatgta ctcggcgatc gtgcgatcgg tgctgaacgc gcccatgccc gcgatgttgc
     1261 gcaacgcgcg ctcggcccag gcctgcggct ggcgatacag cgcgtcgacc tgctcctgcg
     1321 cgtggacgta gtccgcgaag tcggccagca gcatgtacgg atcgcggtgc agcaggggct
     1381 ggacgagcga ccggtagcga tcgggctcgc cccaggagaa caccccgccg ccgatggcgt
     1441 cgaggacttg ttggagtgct tggttttctt ccgcatacag acgcggatcg tatccccagc
     1501 tcttcatctt cgcgacggca tccgtgcgca ggccgaacac gaacatgttc tccacgtcca
     1561 tcgcctcggc catctcgatg ttggcgccgt cccaggtgcc gatggtcaag gcgccgttga
     1621 gcgcgaactt catgttgccg gtgcccgagg cctcggtgcc ggcggtggag atctgctcgc
     1681 tcaggtccgc ggccggcagg atgacctcgg ccaggctgac gccgtagttc ggcaggaaca
     1741 ccaccttcag cttgttgccc acgcgcgggt cggcgttgac gacgcgcgcc acgtcatgga
     1801 tcagctggat ggtcagcttg gccgcgtggt aggccgaggc cgccttgccg gcgaagatga
     1861 cggtgcgagg cacccagtcg gcgccggggt tggcgacgat ggcctggtag cgcgccacca
     1921 cgtgcagcac gttgagcagc tggcgcttgt actcgtggat gcgcttgatc tgcacgtcga
     1981 acaggctgtc cggcgagatc gtgacgccga agtcgcgccg cacgcgctcg gccaggcgct
     2041 ccttgttggc gcgcttgacc tgcaggaatt cctcgccgat cttcctgttg ccggccttgg
     2101 cggccagcgc cttcaggccg tcgaggttgg cgcgccactc gttgccgata tggcgatcga
     2161 gcagcccgct caggcccggg ttggcctgca tcagccagcg ccgcggcgtc acgccgttgg
     2221 tgacgttgag gaagcgctgc gggaacatcg cggcgaaatc ggcgaagatc gtctcgacca
     2281 tcaggcgcga gtgcagcgcc gagacgccgt tgaccttgtg cgacgcgacg atcgccagcg
     2341 acgccatgcg cacgcggcgc tcgccgttct cgtcgatcag cgacaggcgc gcgatcaggc
     2401 ccgtgtcgcc ggggaaccgc gtgcccacct cgtcgaggaa ccggcggttg atctcgtaga
     2461 cgatctcgag gtggcgcggc agcagttcct cgaacatgcg caccggccag gtctccagcg
     2521 cctcgggcat cagcgtgtgg ttggtgtagc tcaccgcctg cgtggtgacg gcccaggcct
     2581 cgtcccagcc catgtggaac tcgtcgagca ggatgcgcat cagctcggcg ggcgcaagcg
     2641 ccgggggcgt gtcgttcagg tggacggcgt tgcggcggcc gaaggtgtcg atctcgccgt
     2701 gctcgcgcag gtggcgcgcg atcatgtcct gcagcgacgc gctgcacagg aagaactcct
     2761 gcttcaggcg cagctggcgg cccgcggcgg tcgagtcgtc ggggtacagc acccagttga
     2821 gcgtgtcggc ggcctcgtag tgctggccgg cggccaggtg ctcgccgcgg cagaacatcg
     2881 cgaagtcgat cgggcgcgac ggctgcgcgt gccactggcg cagcgtggac acgcgctccg
     2941 tggcatggcc cggcacgacg aagtcgcacg cctgcgcgac gacctcctcg gccggcttcc
     3001 acgcgcgccg gccgccggag ctctcgacga cgccgccgaa gcccaccggg tagcgcagct
     3061 cgggccggac gaactcccac gggttgccgt gcctgagcca ttcgtcgggc agctcgacct
     3121 gccggccctc ctggatggcc tgcgcgaaca tgccgtgcgc ataccgcagg ccgtatccga
     3181 aggacggcag ctccagcgtc gcgaacgagt cgaggaagca ggcggccagg cggccgaggc
     3241 cgccgttgcc cagtgccgcg tcgggctcct gctccagcac ctcggccagc gaggccgcgc
     3301 cgctgcgcga atcgagcgcg gcctgcatgg cgccctcgag ccccatcacg gacagcgcgt
     3361 tgcgcagcgc gcggcccatc aggaactcca tcgacaggta gtgcacgcgg cgcgtgccgc
     3421 gcggacgctt cgcatcggcc gcctgcgtgg cggcccagcg gtcggccagc agggggcggc
     3481 aggcgacgac ggcggcgcgc aggcaggcgt cgggcgacgg cgcgtcgctg cgttgcgaga
     3541 gttcgcgctt ccaggctgtg gtgaattctt gggtcaaggc agggtcggct gggtcgcctg
     3601 cgcgcgtgcc cgcaggctcg cgagccgccg tggcgacggt ggtcttgctc attacggaag
     3661 gatccttcta gcgggacggg cggcagcgtg tgacgctcag tcgcccgact gaacaacaac
     3721 agtatcgcgt ttcgcggcgc gcttgtgtag ttctcctaca gagggttatc cctgagcaac
     3781 aacaaaatca cgactttacg aggcaggacc gcgatgtact taatgtagtt tcgcattgat
     3841 cagtggacaa ggtgattgta tattctctac attccgtgcc caagcggcag ccttgctgcc
     3901 gcgcggtgac acccccaaga gacaagcaag cggacgtggc gcacgatccg cccgaggaga
     3961 cgatagaagt ggcccacgta cagctcgtga aggtgcagaa ggcgttcgga gacatccaga
     4021 tcctgcgcga catcgacctg gagatccgcg acggcgagtt catggtgttc gtcggcccct
     4081 cgggcagcgg caagtccacc ctgctgcgca ccatcgccgg cctggaggac atcaccggcg
     4141 gcacgttgtc gatcggcggc aaggtggtca acgacctgcc cccggccgac cgcggcatcg
     4201 cgatggtgtt ccagtcgtac gccctgtacc cgcacatgaa cctgtacgac aacatggc
//
LOCUS       DATCRO010000336         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_161047, whole genome shotgun sequence.
ACCESSION   DATCRO010000336 DATCRO010000000
VERSION     DATCRO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161047"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="pilV"
                     /locus_tag="VIN75_09085"
     CDS             complement(<1..392)
                     /gene="pilV"
                     /locus_tag="VIN75_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY8974358.1"
                     /translation="MHAVSRQRGVSLIEVLMAVLIFSIGLIGLAGLMVMATRSNHSAY
                     QRTQVTFLAGTMADRMGANPVGVWSGFYNSTGYPVAASTASNNDCSSGCTPQALAAAD
                     QRAWSRQLSTFLPNVSAVISCNNGPAGYT"
     gene            complement(595..>2565)
                     /locus_tag="VIN75_09090"
     CDS             complement(595..>2565)
                     /locus_tag="VIN75_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY8974359.1"
                     /translation="GQTLADTSKTVVANRNILTAKEGSDGSFAGGVAFKTFANLDSAG
                     QTLLSGSPASIDATNDTGQTRLDYLRGDRTKETTTFRPRNTLNLLGAIIGSQAVYVSY
                     PSSGYRNSWPAGSPEQTAVAGDAANCATQTPLTCHTYEAFVQNNLSRKPVVYVGANDG
                     MLHAFDASMTTDASGNVVKTATAGTEVFAYVPRAVYPNLGNLTVKDGFKFMPTADGIP
                     VTRDVYFGAATTTPVSTSAGWHTLLVGGLRLGGRGVYALDITDPTAMAAGKVLWEFNA
                     DAPAQAAWSDGSISNPGGNPANLGYTYGQPNIGRLNNGKWVVLVPGGYFPDCTKAPFT
                     GANCTSPAAASNTYSSLFVLDAQTGKLIRELKTPDANSGGAASHGLSSPVLGDYNDDQ
                     VDDVAFAGDLDGNLWRYDLSSSDPNSWAVTLAFKPATAGAQPITSMPRLFADPVTNKF
                     IVVFGTGKYLGVGDNTSGSATTQSVYGIRDNGTTVTRTQLVAQTLSEATADDGKTIAR
                     GLTDNAVDSSKGGWYFDLGPSTSSAGERVVVTPGALFDTGRAVIQTLIPGTNDPCNAS
                     IQGALMVVNAATGGANGGLSAPGVSGWNGTGKYVVGGRVNDPRTTGTVPLVTTVGGGS
                     VLVPGLKLTGSNNVLNINDAVWRRRSWRQIIR"
BASE COUNT          378 a          866 c          883 g          438 t
ORIGIN      
        1 gtatagcccg ccggtccgtt gttgcagctg atcacggcgc tcacgttcgg caggaaggtg
       61 ctgagctggc ggctccaggc gcgctggtcc gcggcggcca gcgcctgcgg ggtgcagccg
      121 gagctgcagt cgttgttcga agccgtgctg gcggctaccg gatagccggt ggagttgtag
      181 aaaccgctcc acacgcctac cgggttggcg cccatgcggt cggccatggt gccggccagg
      241 aaggtgacct gggtgcgctg gtaggccgaa tgattcgaac gcgtcgccat gaccatgagg
      301 ccggccaggc cgatcaggcc aatgctgaag atcagcacgg ccatcagcac ttcgatcagc
      361 gaaacgccgc gctgacgcga cacagcatgc atgacggatt ctcccctgga agcgcggcta
      421 ctctccttag gaggggagaa ttcccacaag cggaatccga caagcggcag gattcgtccg
      481 atgagcggtg aggccgattc ggcgggaatg cgtggcttcg caatagaaat gagcactgga
      541 tcgcactaga cagagcccgc ccgccgggga ccccggcgag gcggatggaa ccgctcagcg
      601 gatgatttgc cgccaggaac ggcggcgcca gacggcgtcg ttgatgttca gaacgttgtt
      661 gctgccggtg agcttcaggc ccggcaccag cacgctgccg ccgcccaccg tcgtcaccag
      721 cggcaccgtg ccggtggtgc gcgggtcgtt cacccggccg cccaccacgt acttgcccgt
      781 gccattccag cccgacacgc ccggcgccga caggccgccg ttcgccccgc ccgtcgccgc
      841 gttcaccacc atcagcgcgc cctggatact cgcgttgcac ggatcgttgg tgcccgggat
      901 caaggtctgg atcaccgcac ggcccgtgtc gaacaaggcc ccgggcgtca ccaccacccg
      961 ctcgccggcc gaggacgtcg acggacccag gtcgaagtac cagccgccct tgctgctatc
     1021 gaccgcgttg tcggtcaggc cgcgcgcgat ggtcttgccg tcgtccgccg tcgcctcgct
     1081 caaggtctgc gccaccagct gggtgcgcgt caccgtcgtg ccgttgtcgc ggatgccgta
     1141 caccgactgc gtcgttgcgc tgccgctggt gttgtcgccc acgcccaggt acttgcccgt
     1201 gccgaacacc acgatgaact tgttggtcac cgggtcggcg aacagccgcg gcatgctggt
     1261 gatcggctgc gcaccggccg tggccggctt gaacgccagg gtcaccgccc agctgttggg
     1321 gtcgctcgag gacaggtcgt agcgccacag gttgccgtcc aggtcgcccg caaaggccac
     1381 gtcgtcgacc tggtcgtcgt tgtagtcgcc cagcaccggc gaggacaggc catggctggc
     1441 cgcgccgccg ctgttggcat ccggcgtctt cagttcgcgg atcagcttgc cggtctgcgc
     1501 atccagcacg aacagcgagc tgtaggtgtt ggaggccgcc gccggcgagg tgcagttggc
     1561 gccggtgaac ggggccttgg tgcaatccgg gaaataaccg cccggcacca gcaccaccca
     1621 cttgccgttg ttgaggcggc cgatgttggg ctggccgtag gtgtagccga ggttggcggg
     1681 gttgccgccc gggttgctga tcgaaccgtc gctccacgcg gcctgcgccg gtgcatcggc
     1741 gttgaattcc cacagcacct tgcccgccgc catcgccgtc gggtcggtga tgtccagcgc
     1801 atacacgccg cggccgccca ggcgcagccc gcccaccagc agggtgtgcc agccggccga
     1861 ggtcgacacc ggcgtggtgg tggcggcgcc gaagtacacg tcgcgcgtca ccggaatacc
     1921 gtcggcggtc ggcatgaact tgaaaccgtc cttcaccgtc aggttgccca ggttcgggta
     1981 gaccgcgcgc ggcacgtagg cgaacacctc cgtgcccgcc gtggcggtct tgaccacgtt
     2041 gccgctggcg tccgtggtca tcgaggcgtc gaacgcatgc agcatgccgt cgttggcgcc
     2101 cacgtacacc accggcttgc ggctgaggtt gttctgcacg aacgcctcgt aggtgtggca
     2161 ggtcagcggc gtctgcgtcg cgcagttcgc cgcatcgccc gccaccgccg tctgctccgg
     2221 cgaaccggcc ggccagctgt tgcggtagcc gctcgacggg tagctcacgt acaccgcctg
     2281 cgagccgatg atcgcgccca gcaggttgag ggtgttgcgc ggacggaagg tggtggtttc
     2341 cttggtgcgg tcgccgcgca ggtagtccag gcgcgtctgg ccggtgtcgt tcgtggcgtc
     2401 gatgctggcc ggcgaacccg acagcagcgt ctgtccggcg ctgtccaggt tggcgaaggt
     2461 cttgaacgcc acgccaccgg cgaagctgcc gtcgctgcct tccttggcgg tcagaatgtt
     2521 gcggttggcc accaccgtct tgctggtgtc ggcaagggtc tgacc
//
LOCUS       DATCRO010000337         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_250954, whole genome shotgun sequence.
ACCESSION   DATCRO010000337 DATCRO010000000
VERSION     DATCRO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250954"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VIN75_09095"
     CDS             complement(<1..455)
                     /locus_tag="VIN75_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY8974360.1"
                     /translation="MFRQLGLIGCGLMGGSFALAMKRAGLVKHVVGYSKSPSTVITAQ
                     KMGVIDQMAESALMAVSGADIVLIAVPVAATEATLRAIRHLVHPNTLFMDVGSTKANV
                     VDAARRVLRERLPSFVPAHPIAGKEVSGITHADATLYQDAQVVLTPVLET"
     gene            843..1160
                     /locus_tag="VIN75_09100"
     CDS             843..1160
                     /locus_tag="VIN75_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEY8974361.1"
                     /translation="MTDLQQLIRQKADLERQIAQLNSKGRQDAIEEIRRIMTEHGLTS
                     DDISAQARGRKAGTKLDGSERKAVAAKYKDDQGNQWTGRGLKPRWLTAALASGKKLED
                     FAV"
     gene            complement(1226..2203)
                     /locus_tag="VIN75_09105"
     CDS             complement(1226..2203)
                     /locus_tag="VIN75_09105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8974362.1"
                     /translation="MRVSVVIPTRNRATLLARAVAGVAAQGHADLELIVVDDGSRPDE
                     AARNAQLVQSVPGARHVVLHPADPQGSGPARARNAGLAAASGALVAFCDDDDQWIAPD
                     FLATAATAFGADERLDLLFANQHAMAGGAIRRTVWQPRLDALLDARGAAVGATTPVSR
                     AECLVDAGDFAHMNTCVFRRSLLEAAGGFDGELRYCEDLDLFVRAVDQARAVACLRRT
                     VAIHHVPDRTRHDNASTRVDAHTRHATLDRIARRLVDRCRTAEALDYSRRLGADACRT
                     LAREAAAGARGHEALAWARRAGDWRPTLRWSIYTQLLRWRALVTPGSAA"
     gene            complement(2356..>3201)
                     /locus_tag="VIN75_09110"
     CDS             complement(2356..>3201)
                     /locus_tag="VIN75_09110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974363.1"
                     /translation="PLDSTRNFLRRARDPALADHAPGRMPAGPAMAAAPRLSLAIVPT
                     TSLTSADWDELWQLTDAFFETDRGWAQDGARRHPLTAMVRSRDDGRLMGSASISMHHV
                     LIGGRMVTAIYTSQVLLRPEARGRNVLQKLGFRCFLAERLRHPFRRIYWFFDTFGWKS
                     YLLLPRNFRDFWPRHDRPTPPRVQSLIDRLASRIHGADWRPAQGVVVRSGRKRLRPAT
                     APLAGARPSPDIDFFLRANPGHAEGDMLVCLCPLSAANWLSAGLRALARGWRRWRRGG
                     RAAGG"
BASE COUNT          491 a         1063 c         1237 g          410 t
ORIGIN      
        1 gtctcgagca ccggcgtgag cacgacctgg gcgtcctggt acagcgtggc gtcggcgtgg
       61 gtgatgcccg agacttcctt gccggcgatc gggtgcgcgg gcacgaagct gggcaggcgc
      121 tcgcgcagca cgcggcgcgc ggcgtcgacg acgttggcct tggtgctgcc cacgtccatg
      181 aacagcgtgt tgggatgcac caggtggcgg atggcgcgca gcgtggcctc ggtggcggcc
      241 accggcacgg cgatcagcac gatgtcggcg ccggagacgg ccatcagcgc cgactcggcc
      301 atctggtcga tgacgcccat cttctgcgcc gtgatcacgg tcgacggcga cttgctgtag
      361 ccgaccacgt gcttcaccag gcccgcgcgc ttcatcgcga gggcgaacga gccgcccatc
      421 aaaccacaac cgatcagccc tagctgcctg aacatctgga ttctttttcc gtggaaaggt
      481 caaaacatgt cgaacccgtt gcaaacccgg acgcgcgaat attcgcggcc cgggaatcca
      541 atcccgacga cccggtcgac atggcgccca tttccggccg cgtcgatgga gttacaaaag
      601 taacagccaa tcggccggcg cgggtgacgg acgcggacga aatggccgtg cgaacacggc
      661 cgatcgccca atttttgcac atccggacgg ctcctccgac ggcggtggcg ggcgttgcga
      721 cgatttcggc ggggctccaa cggtcattcg agggggatga acgacaccca agggacagcc
      781 gtcccggcta aactcggtcg caactgggca tattcgaccc ggccgacacc tcaaggatgg
      841 gtatgactga ccttcagcag ctgattcggc aaaaggccga tctcgagaga caaatcgccc
      901 aactgaattc caaaggtcgc caggacgcca tcgaggaaat tcgccggatc atgaccgagc
      961 acggcctgac ttcggacgac atctccgcgc aggcgcgggg gcgcaaggcc ggcaccaagc
     1021 tcgatggaag cgaacgcaag gccgttgccg ccaagtacaa ggacgaccag ggcaaccagt
     1081 ggacgggccg gggcctgaag ccgcgctggc tgacggcggc gctggcctcc ggcaagaagc
     1141 tggaggactt cgccgtctga gggcgcgggt ccgacatgcg aaggggagcc gcggctcccc
     1201 tttttcatgg cggcggccgt ccggttcagg cggcgctgcc gggcgtgacg agcgcccgcc
     1261 agcgcaggag ttgcgtgtag atcgaccatc gcagcgtcgg ccgccagtcg ccggcgcgcc
     1321 gcgcccaggc cagcgcctcg tggccgcgcg ccccggccgc ggcctcgcgc gccagcgtcc
     1381 ggcaggcgtc ggcgccgagg cgccggctgt agtccagggc ctcggccgtg cggcagcgat
     1441 cgaccagccg gcgggcgatg cggtccagcg tggcgtggcg ggtgtgggcg tcgacgcggg
     1501 tcgacgcgtt gtcgtggcgg gtgcggtcgg ggacgtggtg gatcgccacc gtgcgacgca
     1561 ggcaggcgac ggcgcgcgcc tggtcgacgg cccgcacgaa caggtcgagg tcctcgcagt
     1621 agcgcagttc gccgtcgaag ccgccggcgg cctcgagcag cgagcggcgg aagacgcagg
     1681 tgttcatgtg cgcgaagtcg cccgcgtcga cgaggcattc ggcgcgcgag accggcgtcg
     1741 tcgccccgac cgccgcgccg cgggcgtcga gcagggcgtc gagcctgggc tgccacacgg
     1801 tcctgcggat ggcgccgccg gccatcgcgt gctggttggc gaacagcagg tccaggcgct
     1861 cgtcggcgcc gaaggcggtg gcggcggtgg ccaggaagtc gggcgcgatc cactggtcgt
     1921 cgtcgtcgca gaacgccacc agggcgccgc tggccgcggc gaggccggcg ttgcgggccc
     1981 gcgcggggcc gctgccctgc gggtcggccg gatgcagcac gacgtggcgc gcgcccggga
     2041 ccgactgcac gagctgcgcg ttgcgcgcgg cctcgtccgg cctggagccg tcgtcgacga
     2101 cgatcagttc gaggtcggcg tggccctggg cggcgacgcc cgcgacggcg cgcgcgagga
     2161 gggtggcgcg gttgcgcgtg gggatgacga cggagacgcg cacgggagac gcttgcggcg
     2221 gacggaggac gcggggcgcc gacgatagca gcgggcagtg cgctgcggcg agggccctcg
     2281 tgcgatcgcg tgacggaatt ggcgtcgcgc gcgacggcgt gcgcacgcgc gggtgagggt
     2341 ggggcgcgcg aagcctcagc cgccggcggc ccggccgccc cggcgccacc gccgccagcc
     2401 gcgggccagc gcgcgcaggc cggcgctgag ccagttcgcc gccgacagcg gacacaggca
     2461 cacgagcatg tcgccctcgg catggccggg gttggcgcgc aggaagaagt cgatgtccgg
     2521 cgacgggcgg gcgcccgcga gcggcgcggt cgccggccgc aggcgcttgc ggccggagcg
     2581 cacgacgacg ccctgcgcgg gccgccagtc cgcgccgtgg atgcgcgacg cgaggcggtc
     2641 gatcagcgac tgcacgcgcg gcggcgtggg gcggtcatgg cgcggccaga agtcgcggaa
     2701 gttgcgcggc agcagcaggt agctcttcca gccgaaggtg tcgaagaacc agtagatgcg
     2761 tcggaacgga tggcgcaggc gctcggccag gaagcagcgg aagccgagct tctgcagcac
     2821 gttgcggccg cgcgcctcgg gtcgcagcag cacctgcgag gtgtagatcg cggtgaccat
     2881 gcggccgccg atcaggacat ggtgcatgct gatcgacgcg ctgcccatca ggcgcccgtc
     2941 gtcgcgcgat cgcaccatcg ccgtcagcgg atgacggcgc gcgccgtcct gcgcccagcc
     3001 gcgatcggtc tcgaagaacg catccgtgag ctgccacagc tcatcccagt cggcggacgt
     3061 cagcgacgtc gtcgggacga tggccaagct cagtcgcggc gcggcggcca tggcggggcc
     3121 ggctggcata cggccagggg catggtcggc gagggcgggg tcacgggcgc ggcgcaggaa
     3181 gttgcgtgtc gagtctagtg g
//
LOCUS       DATCRO010000338        29133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_656406, whole genome shotgun sequence.
ACCESSION   DATCRO010000338 DATCRO010000000
VERSION     DATCRO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656406"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(610..3729)
                     /locus_tag="VIN75_09115"
     CDS             complement(610..3729)
                     /locus_tag="VIN75_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974364.1"
                     /translation="MHTRLMLALAVLVAIVAFVSAYVLNERERERRIVELEGRATRIA
                     DLFSRSVAYPLWNLNRAAIDGQLAALAPNPEVAQFSVTTVDDGTVSAVTKIPPSKLVD
                     PIVRTQAIEYTPPGGAKQKIGEVRVVMTRALVDAGIAKARRTTLALAATIVAVLYAAT
                     FVLLRRMVSAPIRRLEGTVDRIAAGDMDARCVSESGDELGRLAARFNSMADRLSDSAR
                     RLRESEATYRGIFENSVEGIFHLDRSGRLNDANAALARLMGHATPAALMAAVNGDDPS
                     GPPLACAPLFTPAQAAAMFAMLQRDGQITGMELQLTRADGTPVWVELNAWRQDRADGD
                     ATTGLHGLITDITTRRQALEDLRSHRDHLEEAVHERTVELADAMKRAEIANQAKSDFL
                     ANMSHEIRTPMNVILGMSRLALLSDPNPQQQNYMQKVHRSAETLLHIINDILDFSKIE
                     AGKLDMEALTFDLGNVMDDLANLVGMKAEEKGLELVFALPENLPDALVGDPSRLGQVL
                     INLCNNAVKFTERGEVVVSVDVLERDAASVRLRFEVRDTGIGIAAEERERLFKPFSQA
                     DTSTSRRFGGTGLGLAISRHLVRLMDGELGVDSAPGRGSRFHFSARFGLGAAAAAPRV
                     PSSSRGLQDCRVLVVDDNDAARDVLVPMARSLGLHPSAANGGRQAVDEVASADARGEP
                     YDLVLLDWKMPGMSGIECAKELARTPLGHPPPTVLMLTAFARDDMARLLAAEQLTVAA
                     TLTKPVTPSTLLNACLQAIGQPAQQPARSELRDEALQSNRAALGGARVLLVEDNPFNQ
                     ELASDLLSRAHVAVQIANNGREAIDLVARERFDAVLMDCQMPVLDGYAATRQLRSDPR
                     WRDLPIIAMTANAIVGDRERVLAAGMNDHIAKPIDVVEMFGTLARWIRRREAAATSGL
                     LHFDREAALAGMMGDDALYRRMLRKFRDREADFATRFDAARADRDLVTATRMAHDLKS
                     VSGSLGATRVQEAADRLEQACTNGASSADIDALLAAVVERLSPVIASLHSLGDGTGST
                     GGAPRPGAA"
     gene            4090..4821
                     /locus_tag="VIN75_09120"
     CDS             4090..4821
                     /locus_tag="VIN75_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8974365.1"
                     /translation="MRRRSIHRAALLALLAPVLQASNAAERVIKVVAAEFPPLTTEAG
                     GHPGGVVLEVVQEACRKIGIALEFHFLPWQRAQLEALGSDDVLIIPFTRTPSREAQYQ
                     WIAPVLEFHTVLVTLTPPPSSIEDARTRVVGYVRGTSFKDEVEHAGFPYVEETDDDVT
                     NAKKLKRGRIGAWITTDLMARGVYRQAGFDPAELMYGPTLGPVKVSWVAASREFPKDV
                     ARRIAAAIEQMRADGSYQAIVKRYQ"
     gene            5077..5853
                     /locus_tag="VIN75_09125"
     CDS             5077..5853
                     /locus_tag="VIN75_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY8974366.1"
                     /translation="MRSHHYTLAAAALLPLALASTGAQAATWGASSPATSSCSASNLF
                     ASWTFGSYTFNNDVWAPCSNVAVGPQTIWANSNADWGVWSDQPNTGGIKSYPHIGYTV
                     NRTISSLSSLTTTCAATTPSGGAWESTFDVWVGNNAHEIMLWLNYTGTSAGCGNVKPI
                     SYNWTSAGCAIPVYTNVSVGGGTWNVYRGNNGSNMVYSFLRTSKTNSTAVDVLAILKY
                     LKNLGWFGDLAVGDVQYGFEITSSSGGLNYSSSNFTVNAR"
     gene            5945..7051
                     /locus_tag="VIN75_09130"
     CDS             5945..7051
                     /locus_tag="VIN75_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY8974367.1"
                     /translation="MLVSATTLAAAGGGASPQVEAIASLPRDAVALVDRAVGRVERPA
                     GAPGRRYQWPGTYFEARFGGDAVYVDLGTGEKHARISIDGREVADLMRPDRRAFRIHG
                     LAPGAHAVRVDIVSESRDGPQTFGGFAVSGPRQAMPPAPARARSIEFIGDSYTVGYGA
                     ASSGRECSDDEVWRTTDNSLAFGPQVARRFDADYRILAISGRGMVRNFANGAGDTLPA
                     AYARWLPGDARPGAAPADEAWSPQLIVIGLGTNDFSVPVAAGEPWSDAAALSAAFESR
                     YVDFVRGLHRRHPTARVLLLATDAGQGAAERAVGRVRGRLAADGVVVGDVLALGPLAL
                     DACNWHPSRADHVSIARRLDAAIEALMPEWRAAP"
     gene            complement(7058..8350)
                     /locus_tag="VIN75_09135"
     CDS             complement(7058..8350)
                     /locus_tag="VIN75_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974368.1"
                     /translation="MSTSTPARSPLTWVPSLYLAMGLPNVTVGVVAAIIFKNLGVSNE
                     DIALYTSQMYLPWVLKPVWSPLLEPFRTKRWWVISMEFTMMAALGLVAFCLPLPGFFA
                     ASLAFFWITGFASATQDIVADGVFMTTMPLRDQARYAGLQGMCWNMGAVIASGLLVSL
                     TGWLHGGLGWSWVHCWMAVMAGTALVMGAFGLWHLRVLPPGAPSGLQGQGLRAAWPAL
                     RESWVTFFRKPHIWTMLAVVFFYRFGEGFIEKFGPLFMLDPRSAGGLGLDNQALGTIN
                     GTVGTIAFIAGAFLGGFVIARMTLRRSLFLLALVLNVPHVTYFYLSHALPDNVAWIGA
                     IVAAEKFGFGMGSVGQMLYMMQQIAPGPFKMTHYAFATGVMALTKWATGSVSGWVYAA
                     FDHHYAPYFAFVLAASIPPLLFAWFAPFPQDERADAPA"
     gene            complement(8367..9953)
                     /locus_tag="VIN75_09140"
     CDS             complement(8367..9953)
                     /locus_tag="VIN75_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY8974369.1"
                     /translation="MSSPTRRDAFKTLVAGTLVVGGSPAATAFPSSSGGIEGQRRADR
                     GDGTYVNPIVPGDHPDPTVLKDGDDYHMTFSSFFSYPGVVIWHSRNLVDWTPIGPALT
                     KPLGTVWAMDLVKHAGRYYIYIPANPGDRQSIFVIHADDIRGPWSDPVDLHIDGCIDP
                     GHAVGEDGKRYLFFNGVRRIGLADDGLSTVGELAHVYDPWRYPADWVVEMFAGEGPKI
                     FRRGEWFYLMAAVGGTSGPPTSHMVIVARSRSIHGPWADCPHNPIVHTRSENEPWWSR
                     GHATAVEGPAGDWWLVYHGYENGFRTLGRQTLLEPLAWTPDGWPRAAGGTLAKPLRKP
                     VPRSTGAAGMALSDDFSTDRFGTQWTFFAPGPREMDRVRREPRALVIAAKGATPADTS
                     PLTMLVGDRRYQAEVTLEPEGGAQAGLTLFYNERMFCGLGFDGTTMATFNYGEEQRWM
                     RQPAAARRVRVRVTNRDHVVTFHYALDGGPWVQHPWQMETSGMHHNVFGGFLSLKIAV
                     HSAGAGQVRVSNFTYRGLPA"
     gene            complement(9967..11418)
                     /locus_tag="VIN75_09145"
     CDS             complement(9967..11418)
                     /locus_tag="VIN75_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974370.1"
                     /translation="MDLSLNEGQAAKPVQADATTDRVPLPQKVGYGLGSILDMWGHWL
                     YPSLSFQVYNIFLGMSPGLISTAQAIKIFVDAASDALFGWASDNTRTRFGRRRPYILV
                     GGVLAGIGLPLMFAVGRGWTDTEYFLFMLVSTLLYVPVMSCFNMPWVSLGSEMTPAYH
                     ERTSVMSIKNAIQKLPELGLFFAAQFTTLAIFRDASGKPDILRGAQVYCTALGVVMVV
                     VTVLIFLLTRERYYEHLVARKQQKIPLADTLWRTLRFRPFRQMLSIALAYAVGTAMVS
                     ALGYYATVYYVCKGDVSLGAAWNFKMGVAGMVFGLAGIPFFNRVARHFGKPRALCLVL
                     TLAIAAFIGDWWFYDPDHPALQLLACGCVAFTGAGFWVLTGSIIADVVDADELESGAR
                     REGSFAACQSWITKVGIAAGVFCSGWILQFTGFDAKLPVQSDHAILMIRILLSSVPVV
                     GLVIGLVVALRFPLTERRMAEIRTRLEARRGTV"
     gene            complement(11453..12544)
                     /locus_tag="VIN75_09150"
     CDS             complement(11453..12544)
                     /locus_tag="VIN75_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8974371.1"
                     /translation="MAKPPAKSTRARHAATLADVGREAGVSIMAASAVLNGAKTSSRI
                     SDETRARILAAAQKLHYRANATARALVDRRMNAIGVATTLSRNELNQYFLEVFNGVIE
                     AAAEFEQNTTVFAVPDWQEGASRIPRICDSRIDGLILLAPLMEIESAKYLPDHTPIVS
                     VHANHEMPNVVNLESDEEAGAFEMVRHMLSLGHRRILHVGGPSDSIGAMRRVSGYLGA
                     HAAAGVKPQDDYIIPGFFSSDSGRRELEAWLQRHRGDPLPQAIFAASDAIAMGCLDTL
                     SARGLRTPADISVVGFDDTMLARSAHLATVRQPLREMGHRAVRLLMDRIEAKHQDTIY
                     RAPSNIVLPTEIVLNHSLAAPRSKALVIS"
     gene            complement(12553..13902)
                     /locus_tag="VIN75_09155"
     CDS             complement(12553..13902)
                     /locus_tag="VIN75_09155"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8974372.1"
                     /translation="MLTYSSPFPPSFVFGVAAASAQIEGAAFEDGKGESIWDRFCRVP
                     GAIHDGDTLDVACDHYHRFDEDFALMASLGVKDYRLSIAWPRLYPDGDGELNRAGVEF
                     YHRLLASMARHGIKPWVTLFHWDLPQALEDRGGWTSRATVDAFARYADTVVKEYGHVV
                     KDWITLNEIRCFTWLGYGAGTKAPGRKESDAVVNQTFHHALLCHGHGVRAVREHGGAG
                     ARVGLTDNCDVSVPVTETPADIAAARAWFVEKNLHVLGAIHGKGYAPRYLERTGPDAP
                     VVRPGDFDLISLPTDFLGLNIYTATYVRAGRDGRPEALALPANYPRADSPWLNLVPQS
                     IYWASRMVHEEYGHRSIYITENGCGYNEEPVVGGEVLDLHRRDYLRSHLREMHRAIAD
                     GVPLDGYFLWSFIDNYEWEDGYQRRFGIVHCDFQTQVRTPKLSARYYAETIRTRCIV"
     gene            complement(13980..15329)
                     /locus_tag="VIN75_09160"
     CDS             complement(13980..15329)
                     /locus_tag="VIN75_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY8974373.1"
                     /translation="MNALVRPFVLLWRGIACRLLALSWALLAGCASFHHAAPADDEVV
                     LRSGKPSDGFSVTLADFDTSLPMTGDSVVLPRAASPHGPDNRVEARATLPGALTMKWH
                     DAWYANLRLDAARPLDLRPFLDDGTLEIDLEAVDMARAGLTFVVGCGPDCERKLPWVV
                     PSRGLVGKGRQHLAIPLRCFVRDGADFSAVTHAFRLDSSGTGEVALSDVRFVRHAKGN
                     VDCPDWRTESVSPRPLEQVWSMDWWMPRHEEKLARIREAAAARHPIDLVFIGDSITHN
                     WEKAGAAVWARHYAKYNALDLGFGGDHTENVLWRLQHGEIDGYRPKVAVVMIGTNNTG
                     DRQEDPATTAAGVRRILDEIRSRQPDTRILLLAVFPRDEQPTSPLRRLNERLSTILSG
                     YADGRHVFFIDIDAALTNADGTLSRDVMPDLLHPDEKGYEIWASKMEPTLLQLMARP"
     gene            complement(15511..17739)
                     /locus_tag="VIN75_09165"
     CDS             complement(15511..17739)
                     /locus_tag="VIN75_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974374.1"
                     /translation="MILRRRFIQQAGALGLLGTGLPAALGAVAAADDAPYDWRSVPFG
                     GGGFIDGFVFHPREKGLLYARTDIGGAYRFDPGTRSWIPLLDQLTKSQTDLSGVLSLA
                     LDPNDANRVYVAGGLYLGSTARNGALLASADRGVTWQINELPIKLGGNSPGRGSGERL
                     QVDPDNGEILYLGTSQDGLMASADRGRSFKPLGFEPRHVSLVLVDPRSGRQGKGSQVL
                     WAGSHDKPGLYVSRDAGRSFERESATPAQAPQHAVFGADGTLYVTFAIGDGTVACNPS
                     YARRGSVWKRSTSGKWTDITPEDPAKDAHGFGYSGIDVDRQVPGRLVVSTIERWSVGD
                     DVFVSNDDGAHWTSVGAHSHHDASAYPWLVDYLNGEDKMGHWLADIKLDPFDGERAIY
                     GTGYGLWLTDNLGAVHKGGTVKWDFAVTNLEETATLEIRSPSGGATLLGAMGDVSGAA
                     WDDTTRTPRNGLFAPTRQTNRAVDFAQLRPAILARTADQAATGGWWSGDGSVTWHPFG
                     KSTRTTTSATGGYLNAGTVAVSAGGSAFVWAPEKQPALCSRDHGKTWVEVAGWPRGEV
                     DAIPVADRQADGVFYTHDRAAGQILTSVDGGLSFRTSVTGLPKLASWQSSQLVSAPGA
                     VRDLWLVLHDTLLHMPGPDKPAVAIRNIVECWKVALGKAAPGRDYHCVYVWGKLQVGD
                     RQVEGLFRSDDAGAHFTRIDDDRHRYGLLLSMTADPLEYGTVYVAPHGRGVVVGKPRR
                     GA"
     gene            complement(17816..19465)
                     /locus_tag="VIN75_09170"
     CDS             complement(17816..19465)
                     /locus_tag="VIN75_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEY8974375.1"
                     /translation="MAVAPLLLAACVGGANAAPAANPAPHLETRDGRTALVVDGAPFL
                     MLGAQANNSSNYPAVLGKVWPALDDLHANTLEIPVAWEQVEPVEGRFDFSYVDTLVTQ
                     AREHGKRLVLLWFGAWKNTSPSYAPEWVKFDNRRFPRMLDRDGKPSYCLSPFGEQTLA
                     ADRKAFVALMTHLAQIDGTRHTVIMMQVENEVGTYGLVRDFGAKAQAAFDKPVPAEVL
                     AHQPAPVKHASHGSWREVYGDYADEYFHAWAIASYIEQVARAGRQSLDIPVYVNNALR
                     DAVAPLAPWNKDFASGGPTYDVIGIYKAAAPHVDIAAPDLYNPASAQISATLAKFQRP
                     DNPLFVPEMSLAPGYSRIVYEVLGRGSLGISPFGIDYTSYTNFPLGAQAAGGPAIVEP
                     FAATYAVFGSMDRLWATWAFEGRTRGVAEGDDRQPQAIALGAWTATVSYQEWQFGEKS
                     WPNHPAEIPPGTDKPSGGVAIAQLGPDEFVITGQHARVRLAPTQPLPADHGAMFARVE
                     EGHFDAAGQWVMEHNWNGDQTDWGLNFTATPVILKVRMGRW"
     gene            complement(19590..22628)
                     /locus_tag="VIN75_09175"
     CDS             complement(19590..22628)
                     /locus_tag="VIN75_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8974376.1"
                     /translation="MRQFKRTALSAAAAQAALLWSGVALAQSAPVPADAASSAPAPAA
                     AASSAPAPAAAASAAQEVTQVVVTGQRAALQSAQKIKQNSDEIVDSIVAEDIGKLPDR
                     SVTEVLQRVVGVTIGHLISNNDPEHYSVEGSGVNIRGLTYVRAELNGRDAFSANGGRS
                     LNFEDVTPELMAGVDVYKNPSAEQIEGGIGGLVNLRTAMPFDFTGQKVSISLSEGYST
                     LAGKKSPKISGLYSNRWDLGSAGQFGALIDLAHSESNTRTDAFQVDPYFNVQSNGQSV
                     WVPESASWRRVDFNNRRNGIYAALQWKKDTVSSSLTYFKSRYDQEWDENAIFTQASTP
                     YNLTVDPGAKFSSGGVFQSGTLRDDADGGLNFGADTRVAKRKSDTQDISWNVNWRASD
                     RWNFNADLQLVKATTRDFDSTVATGTQMAKETLDLSGGLPKLSFDSADLAALANPASY
                     YWAFTMEHFDKGIATEKALKLDARYSFDDPVLSDLRFGIRLTDRGATTENSNPSYNWA
                     AITQTWQKGWDITNLAYLSDPRFSGNTHLVSFSNFMNGKVSVPSLVMPDTSLAAGYPN
                     TYETLHGYTNLLCVSGNTCGNWTPAAYGANNQAGTNDQGERTQTAYGQLRFAWDDWKV
                     PVDGNLGLRMVRTESEAHGYVTYTAPSGIPSGTLTGVTIPTITAFAVEKDFKNTFNDF
                     LPSLNLRAKPRDDLQFRFALARAMTRPDFSQLQAYMPLSTSFKNTGTDITSVSNTGTA
                     TGNPNLRPIKSNQADATAEWYFSRTGSLTFAAFDKELKDIIINQSYIVPVTDTSGNTV
                     NFVTTGPVNGAKGYARGFELAYQQYFDKLPGFLSGFGMQANYTFVNTHTSLYSPVTAT
                     TCGSSNGADNFNLNLNGCDTNGQPFGNLPLQGVSRNSFNFALLYDQGPLSARVAYNWR
                     SKYLQGVNVNGTQGTNGTDPANPTVHNIAWGLPTWADSYGEVDAGIFWKFDDHLTVGL
                     EGTNLTDSTYKQLMQQHIGMMGRAWFTSGPSYNLQLRYTF"
     gene            complement(22884..24203)
                     /gene="xylA"
                     /locus_tag="VIN75_09180"
     CDS             complement(22884..24203)
                     /gene="xylA"
                     /locus_tag="VIN75_09180"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8974377.1"
                     /translation="MTSYFKDIAPITYRGPDSDDALAFRHYDKDRLVLGKRMEDHLRF
                     AVCYWHTFCWNGLDPFGTGTFQRPWFEGGSPMELARVKADAAFDFFARLGAPYYCFHD
                     RDVAPEGATPRESRENFLRMADVLGEHQQRTGMKLLWGTANLFSHRRFMSGAATNPDP
                     AIFALAAVQVRDALEATHRLGGENYVLWGGREGYETLLNTRIGQELDQMGRFLNLVVE
                     HKHKIGFKGTILIEPKPREPSKHQYDFDVATVYGFLVRHGLDKEVKVNIEANHATLSG
                     HSFEHEIATAAALGILGSIDMNRGDPQLGWDTDQFPHNVPDTALAMYHILQAGGLGSG
                     GLNFDAKVRRQSIDAVDLFHGHIGGMDVSARALLVAEQMILDGRLKAAVDARYAGWND
                     AHGQAVLAGRVSLADLADRALAANIDVPPVSGRQEALENLVNRFCGN"
     gene            complement(24237..25712)
                     /gene="xylB"
                     /locus_tag="VIN75_09185"
     CDS             complement(24237..25712)
                     /gene="xylB"
                     /locus_tag="VIN75_09185"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY8974378.1"
                     /translation="MFLGIDLGTSEVKLLLLDADGHAVGMSGAPLEVERPKPLWSEQD
                     PARWWRATNDAVARLRAAHPLKFARVQGIGLSGQMHGAVLLDADDAVLRPAILWNDGR
                     SHAECAELTARVPRLHDIAANLAMPGFTAPKLLWVRRHEPETFKRARSVLLPKDWLRL
                     QLTGEKVSDPSDAAGTLWLDVARRDWSDELLAACGLDRSHMPRLVESNAPSGVLRPEL
                     ARAWGLGPEVVVAGGAGDNAASAIGVGATEPGDGFISLGTSGVLFVVDDRHRANAGSA
                     VHAFCHALPQRWHQMSVMLSAASCLRWFTQFCGAADEPALLAEVAALSPDALASAPLF
                     LPYLAGERTPHNDPFAPGVFYGLSHQSGRALGGYSVLEGVAFGLADGLAALRASGTQV
                     RSLSLVGGGARSALWAQLIADALDVEIVVHRGADAGAALGAARLGWMAAGAGVAEACA
                     KPAVAARFMPDPARRAVLQPRLQQFRQIYGQLKPLWGPAAA"
     gene            complement(25712..27025)
                     /locus_tag="VIN75_09190"
     CDS             complement(25712..27025)
                     /locus_tag="VIN75_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8974379.1"
                     /translation="MAFLAELPRPWPSAAASPRAASAPAARADRADPAGQQRLLKGIN
                     RMTLVRHLCARPGLSRADLAASVQLTKSTVGLLVRELIAEGWLLERESVATGDIGRRP
                     TPLFIDPTRLVLLGAEIGIESTRVVATSLLGEVVAASQVTHGADRGAPACIERLAAAL
                     RALRRELDGAREVIGIGIGVPGGVDETTGFLHFAPNLGWRDVALGALLEGALVDSALA
                     GVPLFVQNEADVAALGELEFDTGTASDPLLYVSINHGVGAGVIVGDRLLIGNRGFAGE
                     IGHTILQPDGPPCSCGRRGCAEALIGVRAMLPARSRAATRRHAAPIAGIARRLAAHDP
                     DTVQAVERAGGHLGTLLQNLAAAYDPACIVLGGAGVDLGDAFIEPALRTLGDYCAAAS
                     LPVPVVRLSQRGADAVAIGAAALVRYRLTRPLIAAIERHAVRGDQ"
     gene            27304..28923
                     /locus_tag="VIN75_09195"
     CDS             27304..28923
                     /locus_tag="VIN75_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8974380.1"
                     /translation="MTDKALRKIVIVGGGTAGWMAAAPLARMLRGGAGAPCGIVLVES
                     PDIGAIGVGEATLPPIRAYNRSLGIDEADFVRRTQASFKLGIEFRDWGRLGHRFFHGF
                     GDFGPSIDNRSSFMHWLRLARAGGMASYEDWSTATVMARANRFTPPRGEVPSAANGYS
                     YAYHFDAALYAAYLREYAMRLGAQRIEGTIVDVRLRTTDGFIESLQLADGRLVDGDLF
                     IDCSGFRGLLIEGALHAGYEDWSDLLPCDSALAVPSARAGALTPYTMSTAKTAGWQWR
                     IPLQHRTGNGHVYCSRFTSDDEAARTLLEGLDTPALGDPRPLRFTTGRRRKSWVRNCV
                     ALGLAAGFVEPLESTSLQLIMDGVGRLVENFPDRDFAPRLADEYNRRIALQYESIRDF
                     IVMHYKLTARDDTAFWRHCAAMPIPDSLQHQIELFRSSGRVAIVDRDGFAEPSFISIL
                     FGLGVEPRSWDPFIDRIDEQALRTHYARLRAAIAGTVDGMPDHAHYIARNARAEPPRM
                     ACTAPAQRMERLPTSADAPNEAGAGNGRIQR"
BASE COUNT         4373 a         9773 c        10475 g         4512 t
ORIGIN      
        1 cgaggcggga gtccgccgca atgggccggc gcgttcgcac ggctggcgag ccaggcccgg
       61 gcctgctcgt cgatgaaggc ggcggcttcg cgccgggtct cgaaggtggg tgcgaatcgg
      121 aagatgcgat cgcaggcttg cgttccgcga ccgctcttga tcgaaagcga tgcacggtag
      181 cgtccctgag tgtccgggac ggtggatgcg gaaagggtgt acttcccgac ttgatagggg
      241 gtgttgattc aggactttca aaggaacgtc gctgccagcg agcgacggcc agagaggagc
      301 gcgcaggctg cgcgcgagca gatgagctct ttccggcaaa gggctggcag ggtttttcgc
      361 tgcaaccgtc agcaaccagg acacggggat tgctggcgca atcgaatggt ccggcatggg
      421 agggagatca actcatgatc tcaaggcgaa ccagactcgt ttgctcgggc gagcaggctg
      481 atccgcgaag aaggcgttca tcctaccacg gccaccctgt cgcgttcccg gcttcgctgc
      541 cgcgtcgaac tgtcgatcac gcttgatgag atgacgcgtc gtgcaagtcg gatccacgat
      601 gcgccgactt caggctgctc caggacgtgg agcgccgcct gtcgatcccg tcccgtctcc
      661 cagcgagtgc aggctggcga tcacggggct gagacgttcg accacggcgg ccagcagcgc
      721 atcgatgtcg gcggaactcg ctccatttgt gcacgcctgc tccaatctat cggcggcctc
      781 ctggacgcgc gtcgccccga ggctgccgga cacgctcttc aggtcgtggg ccatccgcgt
      841 cgccgtcacc aggtcccggt ctgcacgggc tgcgtcgaat cgcgtcgcga agtcggcctc
      901 ccggtcccgg aacttgcgca gcatgcgacg atacagcgcg tcatcgccca tcatgccggc
      961 gagcgcagcc tcgcgatcga agtgcagcaa gccgctcgtc gctgctgcct cacgccgccg
     1021 gatccagcgc gccagcgtgc cgaacatctc gaccacgtcg atcggcttgg cgatgtgatc
     1081 gttcatgccg gccgccagca cgcgttcgcg gtcgccaact atcgcattgg cggtcatcgc
     1141 gatgatgggc agatcgcgcc accgcgggtc gctgcgcagc tggcgcgtgg cggcgtagcc
     1201 gtcgaggacg ggcatctggc aatccatgag aacggcgtcg aaacgttcgc gcgccacgag
     1261 gtcgatggcc tcccgcccgt tgttggcgat ctggaccgcg acgtgcgccc ggctcagcag
     1321 gtcgctcgcc agttcctggt tgaacgggtt gtcctccacg aggagaacgc gcgcgccgcc
     1381 gagcgcggcg cgattgctct gcagcgcctc gtcgcgcagc tcgctcctgg cgggctgctg
     1441 ggcgggctgg ccaatcgcct gcaggcatgc gttcagaagt gtcgatggcg tcaccggctt
     1501 ggtcagcgtc gcggccacgg tcaactgttc cgcggccaga aggcgcgcca tgtcatcgcg
     1561 ggcaaaggcg gtgagcatca gcacggtcgg cggcgggtgc ccaagagggg tgcgagccag
     1621 ttccttggcg cattcgatgc cggacatgcc cggcatcttc caatccagca gaaccaagtc
     1681 atagggctcg ccgcgcgcgt cggccgaggc cacttcatcg acggcctggc gtccgccgtt
     1741 ggcggccgac ggatgcagcc cgagggagcg ggccatgggc acgagcacat cgcgcgcagc
     1801 atcgttgtcg tcgaccacga gcacgcggca gtcctgcagg cccctgctgg aggaaggcac
     1861 gcgaggcgcg gcagctgcgg ctccgagccc gaatctcgcg ctgaagtgga accggctgcc
     1921 gcgccccggc gcgctatcga caccgagttc tccgtccatc aggcgaacga ggtgacggct
     1981 gatggccaag cccagcccgg tgccgccgaa tcgccggctc gtcgatgtgt ctgcttgcga
     2041 gaagggcttg aagagccgct cgcgctcttc ggcagcgatg ccgatccccg tatcccgcac
     2101 ttcgaagcgt agccgcacgg acgcggcgtc ccgctccagc acgtcgacgg agacgacaac
     2161 ctcgcctcgc tcggtgaact tgaccgcgtt gttgcacagg ttgatcagta cctgccccag
     2221 gcgcgatggg tcgcccacca gcgcgtcggg gagattttcc ggcagcgcga acaccagctc
     2281 caggcctttt tcctcggctt tcatgccaac caggttggcg aggtcgtcca tcacgtttcc
     2341 gagatcgaac gtcaaggcct ccatgtccag cttgcctgcc tcgatcttgg agaagtcgag
     2401 aatgtcattg atgatgtgca gcagcgtctc cgccgaccgg tgaaccttct gcatgtagtt
     2461 ctgctgctgc ggattcgggt cgctcagcag ggccagtcgc gacatgccga ggatgacgtt
     2521 catgggcgtg cgaatctcgt ggctcatgtt ggcgaggaag tcgctcttgg cctggtttgc
     2581 gatctcggcc cgcttcatgg cgtcggcgag ttccaccgtt cgctcgtgta ctgcttcctc
     2641 gagatggtca cggtggctgc gcaaatcctc caatgcttgc ctgcgggtgg tgatgtcggt
     2701 gatcaagccg tgcaatccgg tcgtggcgtc gccgtcggct cggtcttgcc gccatgcatt
     2761 gagctcgacc cagacagggg ttccatccgc gcgcgtcagc tggagttcca tgcccgttat
     2821 ctggccatcg cgctggagca tggcgaacat cgccgcggcc tgtgctggcg tgaaaagagg
     2881 cgcgcatgcg agcggcggcc cgctcgggtc gtcgccgttc actgcggcca tcagcgcggc
     2941 tggcgtcgca tgacccatga gtcgcgccaa cgcggcgttg gcgtcgttca accttcccga
     3001 tcgatccagg tggaagatgc cttcgaccga gttctcgaag atccctctgt aagtcgcctc
     3061 gctctcgcgc aaccgccgcg cggagtcgct caggcgatcc gccatcgagt tgaaacgggc
     3121 cgccagtcgg cccagctcgt cgcccgattc gctcacgcag cgcgcgtcca tgtcgccagc
     3181 ggcaatccgg tcgaccgtcc cttcgagccg gcggatgggc gcgctcacca ttcgcctgag
     3241 gagcacgaag gtcgcggcat acagcacggc gacgatcgtt gccgcgagcg ccagcgtggt
     3301 tcggcgcgcc ttggcaatgc ccgcatccac gagcgcccgc gtcatgacga cacggacctc
     3361 gccgatcttc tgcttcgctc ctccgggcgg ggtgtactcg atcgcctggg tccgcacgat
     3421 cggatcgacc agtttcgacg gcgggatctt ggtgactgca gaaaccgtcc cgtcgtcgac
     3481 ggtcgtgacg ctgaactgcg cgacctcggg attgggcgcg agcgcggcga gctgcccgtc
     3541 gatggcggca cggttcaggt tccagagcgg gtaggccacg gaccggctga acaggtctgc
     3601 gatgcgcgtg gcgcgtcctt ccagttccac gatgcgacgc tcgcgctccc gttcgttgag
     3661 cacgtaggct gatacgaagg ccacgatggc gaccagcacc gcaagagcga gcatcagcct
     3721 ggtatgcaag ctggtcgcct gtggaatcag tcggaaccgc gctggcacct catcctctcc
     3781 tcgacgcgtc gagcaggccc tgacccgcgg cgaggcggac cgacgccgat cctcgtgtcg
     3841 gacaggcgcc cggcacgtga tccgcaaccg ggcccggcga cacctcggcc tcacctcgag
     3901 gtccgaaatg gtgtatatct tgatattacg ctggcgcgga tcgggcagac aagccgtcgc
     3961 cggtgttcgg tcgtcgcgcg aaagcccccg acggcgcatc acgcccttgc ccggcgcttc
     4021 gtcttgtcgg gacggtgcgg acgaacagga ccggttgact gcctgggctc ttctcgaagg
     4081 agactccgca tgcgaagacg gtcgatccac cgcgcagcct tgctcgcgct gcttgcaccg
     4141 gttcttcagg catcgaacgc ggccgaacgg gtcatcaagg tcgtcgcggc ggagtttcct
     4201 ccgctgacga cagaggctgg cggacacccg ggcggcgtcg tcctggaggt ggtccaggag
     4261 gcgtgcagga agatcggcat cgcgctcgaa ttccactttc tgccgtggca gcgagcccag
     4321 ctggaagcac ttggcagcga cgacgtactc atcattccct tcacccggac gccgagcagg
     4381 gaagcgcaat accaatggat cgcgcccgtg ctggagttcc acaccgtcct ggtgaccctc
     4441 acgccaccgc cttcgtcgat cgaagacgcg cggacgcgag tggtgggtta tgtgcgtggc
     4501 accagcttca aggacgaggt cgagcatgcg ggatttccgt acgtcgagga gaccgacgac
     4561 gatgtgacga acgcaaagaa actgaagcgg gggcgcatcg gtgcatggat cacgaccgac
     4621 ctcatggcgc gcggcgtcta ccgtcaggcc ggattcgatc cggccgaact gatgtacggc
     4681 ccgacgttgg ggccggtcaa ggtttcatgg gtcgcggcgt cccgggagtt tccaaaggat
     4741 gtcgccagga ggatcgcggc cgccatcgag cagatgaggg cggatgggtc gtaccaggcg
     4801 atcgtcaagc gctaccagtg agctgcgggc aactcgatcg gctggtgatc gtcacgcgac
     4861 agcgtgcagc cgacgtctga ccgcttgcgc cccggaggcg ttcgcatcat gcgatcaccg
     4921 ggtccgggtc agcccgggtg ttcgaacaaa ttcttcgccg atggaatggc cttcgcgtcg
     4981 gatgacagcg ttgccaactg gaggtgcgtc gtccgcgcgg agccgtcgca gaacggtcat
     5041 ggcgacttcc accaacatcc accattccgg agacatatgc gttcgcatca ctacacactc
     5101 gctgcagccg cgctgcttcc actggccctc gcctcgacgg gcgcgcaggc cgcgacctgg
     5161 ggggcgtcgt cgcccgccac gtcgtcgtgc tcggcttcga acctcttcgc cagctggaca
     5221 ttcggcagct acacgttcaa caacgacgtc tgggcgccgt gcagcaacgt cgcggtgggg
     5281 ccccagacga tctgggccaa ctccaatgcc gactggggcg tctggtcgga ccagccgaac
     5341 accggcggca tcaagtcgta cccgcacatc ggctacacgg tcaaccgcac catcagctcg
     5401 ctgtcctcgc tcaccacgac ctgcgcagcg acgacgcctt cgggcggcgc gtgggaatcg
     5461 acgttcgacg tctgggtcgg gaacaacgcg cacgagatca tgctgtggct caactacacc
     5521 ggcacgtcgg ccggctgcgg gaacgtcaag ccgatctcgt acaactggac gtcggccggc
     5581 tgcgcgatcc ccgtctacac gaacgtgagc gtgggcggcg ggacgtggaa cgtctaccgc
     5641 ggcaacaacg gcagcaacat ggtgtattcg ttcctgcgca cgagcaagac caacagcact
     5701 gccgtcgacg tgctcgcgat cctcaagtac ctgaagaacc tgggctggtt cggcgacctg
     5761 gccgtgggcg acgtgcagta cggcttcgag atcacctcgt cgtcgggcgg cctgaactac
     5821 agttcgtcca acttcacggt gaacgccagg tgacgcggac gccggcgtgg cgcgcgccgc
     5881 gccggcgctc gcggagcaac tgtccatggc gaccacgccc cttcttcgaa aggccttgac
     5941 gctcatgctc gtcagcgcga cgaccctggc ggcggccggc ggcggcgctt cgccgcaggt
     6001 cgaggcgatc gcctcgctgc ctcgcgatgc ggtcgcgctg gtcgaccggg ccgtcgggcg
     6061 cgtcgagcgc ccggccggcg cgccgggccg ccgctaccag tggcccggca cctacttcga
     6121 ggcgcgcttc ggcggcgacg ccgtctacgt cgacctgggc accggcgaga agcacgcgcg
     6181 catctcgatc gacggccgcg aggtcgccga cctcatgcgg cccgaccggc gcgcatttcg
     6241 catccacgga ctggcgcctg gcgcgcatgc ggtgcgcgtg gacatcgtga gcgaaagccg
     6301 cgacggcccg cagaccttcg gcgggtttgc ggtttccggg cctcggcagg cgatgccacc
     6361 ggcgccggcc cgcgcacgct cgatcgagtt catcggcgac tcctacaccg tcggctacgg
     6421 cgccgcctcg agcggacgcg agtgcagcga cgacgaggtc tggcgcacga cggacaactc
     6481 gctggccttc ggtccgcagg tcgcgcgccg cttcgatgcc gactaccgga tcctggcgat
     6541 ctccggccgc ggcatggtgc gcaacttcgc caacggcgcg ggcgacacgc tgccggcggc
     6601 gtacgcgcgc tggttgcccg gcgatgcgcg ccccggcgcg gcacctgccg acgaagcctg
     6661 gtcgccgcag ctcatcgtga tcggcctggg caccaacgat ttctcggtgc ccgtggccgc
     6721 cggcgaaccg tggtccgacg ccgcggcgct gtcggcggcg ttcgaatcgc gctacgtcga
     6781 cttcgtgcgc ggcctgcatc gccgccatcc gacggcgcgc gtgctgctgc tggccaccga
     6841 cgccggccaa ggcgccgcgg agcgcgcggt cggccgcgtg cgtgggcgcc tggcggcgga
     6901 cggggtcgtc gtgggcgacg tgctcgcgct gggcccgctc gcgctcgacg cgtgcaactg
     6961 gcatccctcg cgcgccgacc acgtgtcgat cgcgcgccgg ctcgacgcgg cgatcgaggc
     7021 gctgatgccc gagtggcgcg cggcgccctg agcggcgtca ggcgggcgcg tccgcgcgct
     7081 cgtcctgcgg gaacggcgcg aaccacgcga acagcagcgg cgggatcgac gcggcgagca
     7141 cgaacgcgaa gtagggcgcg tagtggtggt cgaacgccgc gtacacccag ccgctgaccg
     7201 agccggtggc ccacttggtc agcgccatga cgccggtcgc gaacgcatag tgcgtcatct
     7261 tgaacgggcc cggcgcgatc tgctgcatca tgtagagcat ctggccgacc gagcccatgc
     7321 cgaagccgaa cttctcggcg gcgacgatcg cgccgatcca ggcgacgttg tcgggcagtg
     7381 cgtggctcag gtagaagtag gtcacgtgcg gcacgttcag cacgagggcc agcaggaaca
     7441 acgaacgccg cagcgtcatg cgcgcgatca cgaagccgcc gaggaacgcg ccggcgatga
     7501 aggcgatggt gcccaccgtg ccgttgatgg tgcccagcgc ctggttgtcc aggcccaggc
     7561 cgcccgcgct gcgcgggtcg agcatgaaca gcggcccgaa cttctcgatg aagccctcgc
     7621 cgaagcggta gaagaagacg acggccagca tcgtccagat gtgcggcttg cggaagaacg
     7681 tcacccacga ttcgcgcagc gcgggccatg cggcgcgcag cccctggccc tgcaggcccg
     7741 acggcgcgcc gggcggcagc acgcgcaggt gccacaggcc gaaggcgccc atcacgagcg
     7801 cggtgccggc catcacggcc atccagcagt gcacccacga ccatccgagt ccgccatgca
     7861 gccatcccgt gagcgagacc agcaggcccg acgcgatcac cgcgcccatg ttccagcaca
     7921 tgccctgcag cccggcgtag cgcgcctggt cgcgcagcgg catggtcgtc atgaacacgc
     7981 cgtcggcgac gatgtcctgc gtggccgacg cgaagccggt gatccagaaa aaggccagcg
     8041 acgctgcgaa gaagccgggc agcggcaggc agaacgcgac caggccgagc gcggccatca
     8101 tcgtgaactc catgctgatc acccaccagc gcttggtgcg gaagggctcg agcagcggcg
     8161 accacacggg cttgagcacc cagggcaggt acatctgcga ggtgtagagc gcgatgtcct
     8221 cgttggagac gccgaggttc ttgaagatga tcgcggcgac cacgccgacc gtcacgttgg
     8281 gcaggcccat cgcgaggtag aggctgggca cccaggtcag cggcgagcgg gcgggagtgg
     8341 aagtggacac gttgggggaa ccgggttcag gccggcaggc cgcggtaggt gaagttgctg
     8401 acgcgcacct ggccggcccc ggcgctgtgc acggcgatct tcaggctcag gaagccgccg
     8461 aagacgttgt ggtgcatgcc cgaggtctcc atctgccacg ggtgctgcac ccacgggccg
     8521 ccgtcgagcg cgtagtggaa ggtgacgacg tggtcgcggt tggtcacgcg cacgcgcacg
     8581 cggcgcgcgg cggcgggctg gcgcatccag cgctgctcct cgccgtagtt gaacgtggcc
     8641 atcgtcgtgc cgtcgaagcc caggccgcag aacatgcgct cgttgtagaa cagcgtgagg
     8701 ccggcctgag cgccgccctc gggctcgagc gtgacctcgg cctggtagcg gcggtcgccg
     8761 acgagcatgg tcagcggcga ggtgtcggcc ggcgtcgcgc ccttggcggc gatcacgagg
     8821 gcgcgcggct cgcggcgcac gcgatccatc tcgcgcgggc cgggcgcgaa gaacgtccat
     8881 tgcgtgccga agcggtccgt cgagaagtcg tcggacagcg ccatgcccgc cgcgccggtc
     8941 gaacgcggca cgggtttgcg cagcggcttg gcgagcgtgc cgccggccgc gcgcggccag
     9001 ccgtcgggcg tccaggcaag cggctccagc agcgtctggc ggccgagcgt gcgaaagccg
     9061 ttctcgtagc cgtggtagac cagccaccag tcgcctgccg ggccttcgac ggccgtcgcg
     9121 tggccgcgcg accaccacgg ttcgttctcg ctgcgcgtgt gcacgatcgg gttgtgcggg
     9181 cagtcggccc aggggccgtg gatcgagcgc gaacgcgcga cgatcaccat gtggctggtc
     9241 ggcgggcccg aggtgccgcc caccgcggcc atcaggtaga accactcgcc acgccggaag
     9301 atcttcggcc cctcgccggc gaacatctcc acgacccagt cggccggata gcgccagggg
     9361 tcgtagacgt gcgccagctc acccacggtg gacagcccgt cgtccgccag gccgatgcgg
     9421 cgcacgccgt tgaagaacag gtaccgcttg ccgtcctcgc cgaccgcgtg gcccgggtcg
     9481 atgcagccgt cgatgtgcag gtcgaccggg tcgctccacg ggccgcggat gtcgtcggcg
     9541 tggatgacga agatcgactg ccggtcgccc gggttggccg ggatgtagat gtagtagcgg
     9601 ccggcgtgct tgaccaggtc cattgcccag acggtgccca gcggcttggt cagcgccggg
     9661 ccgatcggcg tccagtcgac caggttgcgc gagtgccaga tgacgacgcc ggggtacgag
     9721 aagaacgacg agaacgtcat gtggtagtcg tcgccgtcct tgagcaccgt cggatcggga
     9781 tggtcgcccg gcacgatggg gttcacgtac gtgccgtcgc cgcggtcggc gcggcgctgg
     9841 ccttcgatgc cgccgctcga cgacgggaag gcggtcgcgg ccgggctgcc gccgacgacc
     9901 agcgtgccgg ccacgagggt cttgaacgcg tcgcggcgtg tgggagaact catgcgcctt
     9961 gctccgtcac acggtgccgc ggcgcgcctc gaggcgcgtg cggatctctg ccatgcgccg
    10021 ctccgtcagc gggaagcgca gcgccacgac cagcccgatc acgagcccca ccaccgggac
    10081 gctggacagc aggatgcgga tcatgaggat cgcgtgatcg ctctgcaccg gcagcttcgc
    10141 gtcgaagccg gtgaactgca ggatccagcc cgagcagaac acgccggcgg cgatgccgac
    10201 cttggtgatc caggactggc aggcggcgaa cgagccttcg cggcgggctc cgctctcgag
    10261 ctcgtcggca tcgaccacgt ccgcgatgat cgagccggtc agcacccaga agcccgcgcc
    10321 ggtgaacgcg acgcacccgc acgcgagcaa ctgcagcgcg gggtggtccg ggtcgtagaa
    10381 ccaccagtcg ccgatgaacg cggcgatggc cagcgtcagc accaggcaca gcgcgcgcgg
    10441 cttgccgaag tggcgcgcga cgcggttgaa gaacgggatg ccggccaggc cgaacaccat
    10501 gcccgccacg cccatcttga agttccatgc cgcgcccagg gacacgtccc ccttgcagac
    10561 gtagtagacc gtcgcgtagt agcccagggc gctgaccatc gcggtgccga cggcgtaggc
    10621 cagcgcgatc gacagcatct ggcggaacgg ccggaagcgc agcgtgcgcc acagggtatc
    10681 ggccaagggg atcttctgct gcttgcgggc gaccaggtgc tcgtaatagc gctcccgcgt
    10741 gagcaggaag atgagcaccg tgacgacgac catcaccacg cccagcgccg tgcagtagac
    10801 ctgcgcgccg cgcaggatgt cgggcttgcc gctcgcgtcg cgaaagatgg ccagcgtcgt
    10861 gaactgcgcg gcgaagaaca gccccagctc gggcagcttc tggatcgcgt tcttgatcga
    10921 catcacgctg gtgcgctcgt ggtacgcggg cgtcatctcc gagcccaggc tgacccacgg
    10981 catgttgaag cagctcatca ccggcacgta cagcagcgtg gagacgagca tgaacaggaa
    11041 gtattcggtg tccgtccagc cgcggcccac ggcgaacatc agcggcagcc cgatgcccgc
    11101 caggacgccg cccaccagga tgtagggccg gcggcggccg aagcgcgtgc gggtgttgtc
    11161 cgaggcccat ccgaacagcg catcggaggc cgcgtcgacg aagatcttga tggcctgcgc
    11221 cgtggagatc aggcccggcg acatgcccag gaagatgttg tagacctgga acgacagcga
    11281 cgggtagagc cagtggcccc acatgtccag gatggagccg agcccgtagc ccaccttctg
    11341 gggcagcggc acgcggtccg tcgtggcgtc ggcctggacc ggtttggccg cctggccttc
    11401 atttagcgat aaatccatgg tggccatcgt ggtgtgtctt gcgcaggaag ggtcaggaga
    11461 tgacaagggc cttggaccgc ggcgcggcca gggagtggtt caggacgatc tctgtgggca
    11521 gcacgatgtt agagggcgcc cggtagatcg tgtcctggtg ttttgcctcg atgcggtcca
    11581 tcagcagccg cacggcgcgg tggcccatct cgcgcagcgg ctggcgcacc gtggccaggt
    11641 gcgctgatcg cgcgagcatc gtgtcgtcga agccgacgac ggagatgtcc gccggcgtgc
    11701 gcaggccgcg cgcggacagc gtgtcgaggc agcccatcgc gatggcgtcg ctggccgcga
    11761 agatcgcttg cggcagcggg tcgccgcggt ggcgctgcag ccaggcctcc agctcccgcc
    11821 ggcccgagtc gctgctgaag aagccgggga tgatgtagtc gtcctgcggc ttgacgcccg
    11881 ccgcggcgtg cgcgcccagg tagcccgaga cgcggcgcat cgcgccgatc gagtccgacg
    11941 gcccgcccac gtgcaggatg cgccggtggc ccagcgacag catgtggcgc accatctcga
    12001 acgcaccggc ctcctcgtcc gactcgaggt tcaccacgtt gggcatctcg tggttggcgt
    12061 gcaccgagac gatgggcgtg tggtccggca ggtacttggc cgattcgatc tccatcagcg
    12121 gcgccaggag gatcaggccg tcgatgcggc tgtcgcagat cctgggaatc cggctcgcgc
    12181 cttcctgcca gtcgggcacc gcaaagacgg tggtgttctg ctcgaattcc gccgcggcct
    12241 cgatcacgcc gttgaacact tccaggaagt actggttgag ctcgttgcgc gacagcgtcg
    12301 tcgccacgcc gatcgcgttc atgcggcggt cgaccagcgc gcgcgccgtg gcgttggctc
    12361 gatagtgcag cttctgggcg gctgccagga tgcgggcgcg cgtctcgtcg gagatgcgcg
    12421 aggacgtctt ggccccgttc aggaccgccg aggcggccat gatggacacg cccgcctcgc
    12481 gccccacgtc ggccagcgtg gcggcgtgtc gggcgcgggt ggacttggcg ggcggtttgg
    12541 ccatgggcaa ggtcagacga tgcagcgggt gcggatggtt tcggcgtaat agcgggcgga
    12601 cagcttgggc gtgcgcacct gcgtctggaa gtcgcagtgc acgatgccga agcggcgctg
    12661 gtagccgtcc tcccactcgt aattgtcgat gaaagaccac aggaagtagc cgtccagcgg
    12721 gacgccgtcc gcgatggccc ggtgcatctc gcgcaggtgg ctgcgcaggt agtcgcggcg
    12781 atgcaggtcc agcacctcgc cgccgacgac cggctcctcg ttgtagccgc agccattctc
    12841 cgtgatgtag atcgagcggt ggccatattc ctcgtgcacc atgcgcgagg cccagtagat
    12901 cgactgcggc acgaggttca gccacgggct gtcggcgcgc gggtagttcg ccggcagcgc
    12961 cagcgcctcg gggcggccgt cgcggccggc gcgcacgtag gtggccgtgt agatgttcag
    13021 gccgaggaag tcggtgggca ggctgatcag gtcgaagtcg cccgggcgca cgacgggcgc
    13081 gtcgggaccg gtgcgctcga ggtagcgcgg cgcgtagccc ttgccgtgga tcgcgcccag
    13141 cacgtgcagg ttcttctcga cgaaccaggc acgcgcggcg gcgatgtcgg ccggcgtctc
    13201 ggtgacgggc acgctcacgt cgcagttgtc ggtcaggccc acgcgcgcgc cggcgccgcc
    13261 gtgctcgcgc accgcgcgca cgccgtggcc gtggcacagc agagcgtggt ggaaggtctg
    13321 gttgacgacg gcgtcgctct ccttgcggcc cggcgccttg gtgcccgcgc cgtagcccag
    13381 ccacgtgaag cagcggatct cgttgagcgt gatccagtcc ttgacgacgt ggccgtactc
    13441 cttgacgacc gtgtcggcat agcgcgcgaa ggcgtcgacc gtcgcgcgcg acgtccagcc
    13501 gccacgatcc tccagcgcct gcggcaggtc ccagtggaac agcgtgaccc agggcttgat
    13561 gccgtggcgc gccatgctcg cgagcaggcg atggtagaac tcgacgccgg cgcggttgag
    13621 ctcgccgtcg ccgtccgggt acaggcgcgg ccacgcgatc gacaggcggt agtccttgac
    13681 gcccagcgag gccatcagcg cgaagtcctc gtcgaagcgg tggtagtggt cgcaggcgac
    13741 gtcgagcgtg tcgccgtcgt ggatcgcgcc ggggacgcga cagaagcggt cccagatgga
    13801 ctcgcccttg ccgtcctcga aggcggcccc ctcgatctgg gcggacgcgg cggccacgcc
    13861 gaagacgaac gagggaggaa acggactgga ataggtgagc atggaaagcg gatcggaatc
    13921 ggaggggtga ggggaatgcc gcgtggtcgc ggcgtctggt cgtctctggt cgagggcgtt
    13981 cagggccggg ccatcagctg gagcagcgtc ggctccatct tgctggccca gatctcgtag
    14041 cccttctcgt cgggatggag caggtcgggc atgacgtcgc gcgacagcgt gccgtcggcg
    14101 ttcgtcagcg ctgcgtcgat gtcgatgaag aagacgtggc gcccgtcggc gtagccggac
    14161 aggatcgtgc tgaggcgctc gttgaggcgg cgcagcgggc tcgtcggctg ctcgtcgcgc
    14221 gggaacacgg ccagcagcag gatgcgcgtg tcgggttgac gcgaccggat ctcgtcgagg
    14281 atgcgccgca cgccggccgc cgtggtggcc gggtcctcct ggcggtcgcc ggtgttgttg
    14341 gtgccgatca tcacgaccgc gaccttgggc cggtagccgt cgatctcgcc gtgctgcagg
    14401 cgccacagca cgttctcggt gtggtcgccg ccgaagccca gatccagcgc gttgtacttc
    14461 gcgtagtgcc gcgcccagac ggccgcgccg gccttctccc agttgtgggt gatggagtcg
    14521 ccgatgaaga cgaggtcgat cgggtggcgc gcggcggccg cctcgcggat gcgcgcgagc
    14581 ttctcctcgt ggcgcggcat ccaccagtcc atcgaccaca cctgctcgag cggccgcggc
    14641 gagacggatt ccgtcctcca gtcggggcag tcgacgttgc ccttggcgtg gcgcacgaag
    14701 cgcacgtccg acagggcgac ctcgcccgtg ccgctggagt ccagccggaa cgcgtgcgtg
    14761 acggcgctga agtcggcgcc gtcgcgcacg aagcagcgca gcgggatggc caggtgctgc
    14821 cggcccttgc ccaccaggcc gcgcgacggc acgacccacg gcagcttgcg ctcgcagtcg
    14881 gggccgcagc ccacgacgaa ggtcaggccg gcccgggcca tgtcgacggc ctcgaggtcg
    14941 atctccaacg tgccgtcgtc gaggaaggga cgcaggtcca ggggacgcgc ggcgtccaag
    15001 cgcaggttcg cgtaccaggc gtcgtgccac ttcatcgtca gcgcgcccgg gagcgtggcg
    15061 cgggcctcga cgcggttgtc cgggccgtgc ggcgaggcgg cccgcggcag cacgacggag
    15121 tccccggtca tcggcaggct cgtgtcgaag tcggcgagcg tcacggaaaa gccgtccgac
    15181 ggcttgccgc tgcgcagcac cacctcgtcg tcggccgggg cggcgtggtg gaacgacgcg
    15241 cagccggcca gcagggccca ggacagggcg agcagccggc aggcgatgcc gcgccagaga
    15301 aggacgaagg gacggacgag ggcattcatg ggggggcgca gaggcgactc gcccagccgg
    15361 ccgttgccga ccggcagcgc ctcgcattcc cggccggcgt gcgtcgcggg cgccggcggg
    15421 tcgtggcgca gcgcgggatc ggcgcatgcg gccgaacgcg tcgcgcaggt ggcgacgagc
    15481 gagaggagcg agggaagcgc gcccatggcc tcaggcgccc cggcgtggct tgccgacgac
    15541 cacgccgcgc ccatgcggcg cgacatacac cgtgccgtat tcgagcggat cggccgtcat
    15601 cgagagcagc aggccgtagc ggtggcggtc gtcgtcgatg cgcgtgaaat gcgcgccggc
    15661 gtcgtccgag cggaacaggc cttcgacctg gcgatcgccc acctgcagct tgccccacac
    15721 gtagacgcag tgatagtccc ggccgggcgc ggccttgccg agcgccacct tccagcattc
    15781 gacgatgttg cggatcgcga cggccggctt gtccgggccg ggcatgtgca gcagcgtgtc
    15841 gtgcagcacc agccacaggt cgcgcaccgc accgggcgcg gacacgagct gcgaggactg
    15901 ccagctcgcg agcttgggca ggccggtgac gctggtgcgg aagctcaggc cgccgtcgac
    15961 gctggtcagg atctggccgg ccgcgcggtc gtgcgtgtag aagacgccgt cggcctggcg
    16021 atcggccacg gggatcgcgt cgacctcgcc gcgcggccag ccggccacct cgacccaggt
    16081 cttgccatgg tcgcgcgagc acagcgcggg ctgcttctcg ggcgcccaga cgaacgcgct
    16141 gccgccggcc gacacggcga ccgtgcccgc gttgaggtag ccgcccgtgg ccgacgtcgt
    16201 cgtgcgcgtc gacttgccga acgggtgcca ggtgaccgag ccgtcgccgg accaccagcc
    16261 acccgtggcc gcctggtcgg cggtgcgcgc gaggatggcg gggcgcagct gcgcgaagtc
    16321 caccgcgcgg ttggtctgcc gggttggggc gaacaggccg ttgcgcggcg tcctggtcgt
    16381 gtcgtcccac gccgcgccgc tgacatcgcc catcgcgccc agcagcgtcg cgccgcccga
    16441 ggggctgcgg atctcgagcg tggccgtctc ctcgaggttg gtcacggcga agtcccactt
    16501 gacggtgccg cccttgtgca cggcgcccag gttgtcggtg agccacaggc cataccccgt
    16561 gccgtagatg gcgcgctcgc cgtcgaacgg atcgagcttg atgtcggcca gccagtggcc
    16621 catcttgtcc tcgccgttca ggtagtccac gagccaggga tacgcgctcg cgtcgtggtg
    16681 cgagtgcgcg ccgaccgacg tccagtgcgc gccgtcgtcg ttcgagacga acacgtcgtc
    16741 gccgacgctc cagcgctcga tggtcgagac cacgagccgg cccggcacct ggcgatcgac
    16801 gtcgatgccc gagtagccga atccgtgcgc gtccttggcc ggatcctcgg gggtgatgtc
    16861 cgtccacttg ccggacgtgc tgcgcttcca cacgctgccg cgcctggcgt agctcgggtt
    16921 gcacgccacc gtgccgtcac cgatggcgaa tgtgacgtac agcgtgccgt cggcgccgaa
    16981 caccgcgtgc tgcggcgcct gcgcgggcgt ggcggactcg cgctcgaagc tgcggccggc
    17041 gtcgcgcgac acgtacaggc ccggcttgtc gtggctgccc gcccacagca cctgcgagcc
    17101 cttgccctgg cggccgctgc gcgggtcgac cagcaccagc gagacatggc gcggctcgaa
    17161 gccgagcggc ttgaagctgc ggccgcggtc ggcgctggcc atcaggccgt cctgcgacgt
    17221 cccgaggtag aggatctcgc cgttgtccgg gtcgacctgc aggcgctcgc ccgagccgcg
    17281 gcccggcgag ttgccgccca gcttgatcgg caactcgttg atctgccagg tcacgccgcg
    17341 gtcggcggag gccagcaggg cgccgttgcg cgcagtcgag ccgaggtaca ggccgccggc
    17401 cacgtagacg cggttcgcgt cgttcggatc gagcgcgagg ctcagcacgc ccgacaggtc
    17461 ggtctgcgac ttcgtcagct ggtccagcag cgggatccac gagcgcgtgc cggggtcgaa
    17521 gcggtaggcg ccaccgatgt cggtgcgcgc gtacagcagg cccttctcgc gcggatggaa
    17581 cacgaagccg tcgatgaagc cgccgccgcc gaacggcacg ctgcgccagt cgtagggggc
    17641 gtcgtcggcg gccgcgaccg cgccgagcgc ggcgggcagg ccggtgccga gcaggccgag
    17701 cgcgccggcc tgctggatga aacgacgtct gaggatcatg caatgtctcc aacgagggcc
    17761 cgcgcgctcg cggcggcggc ctggcttgtg cttgtcgatg gcgcggcgga gtgcgtcacc
    17821 agcggcccat gcgcaccttg aggatcacgg gcgtggccgt gaagttcagg ccccagtcgg
    17881 tctggtcgcc gttccagttg tgctccatca cccactgccc ggcggcgtcg aagtggcctt
    17941 cctcgacgcg cgcgaacatc gcgccgtggt cggccggcag cggctgcgtg ggcgcgaggc
    18001 gcacgcgggc gtgctggccg gtgatcacga attcgtcagg accgagctgc gcgatcgcca
    18061 cgccgccgct gggcttgtcg gtgcccggcg ggatctcggc cggatggttc ggccaggact
    18121 tctcgccgaa ctgccactcc tggtacgaca cggtcgccgt ccacgcgccg agcgcgatcg
    18181 cctgcggctg gcggtcgtcg ccctcggcca cgccgcgcgt gcggccctcg aaggcccagg
    18241 tcgcccacag ccggtccatc gagccgaaca ccgcataggt cgccgcgaag ggctcgacga
    18301 tggcggggcc gccggccgcc tgcgcgccga gcgggaagtt ggtgtagctc gtgtagtcga
    18361 tgccgaaggg cgagatgccc agcgatccgc ggcccagcac ctcgtagacg atgcgcgaat
    18421 agcccggcgc gaggctcatc tcgggcacga acagcgggtt gtccgggcgc tggaacttcg
    18481 cgagcgtcgc ggagatctgc gccgacgccg ggttgtagag atcgggcgcg gcgatgtcca
    18541 cgtgcggcgc cgcggccttg tagatgccga tgacgtcgta ggtcggaccg ccgctggcga
    18601 agtccttgtt ccagggcgcg agcggcgcca ccgcgtcgcg cagcgcgttg ttcacgtaga
    18661 cggggatgtc gagcgactgg cggcccgcgc gcgccacctg ctcgatgtag ctggcgatgg
    18721 cccaggcgtg gaagtactcg tcggcgtagt cgccgtagac ctcgcgccag ctgccatgcg
    18781 aggcgtgctt gacgggcgcg ggctggtgcg cgagcacctc ggccggcacc ggcttgtcga
    18841 acgcggcctg cgccttcgcg ccgaagtcgc gcacgaggcc gtaggtgccg acctcgttct
    18901 ccacctgcat catgatgacg gtgtggcgcg tgccgtcgat ctgcgcgagg tgggtcatca
    18961 gcgcgacgaa ggccttgcgg tcggcggcca gcgtctgctc gccgaagggc gacaggcagt
    19021 agctcggctt gccgtcgcgg tcgagcatgc gcgggaagcg cctgttgtcg aacttgaccc
    19081 attcgggcgc gtacgacggg ctcgtgttct tccaggcgcc gaaccacagc agcaccaggc
    19141 gcttgccgtg ctcgcgcgcc tgggtcacga gcgtgtcgac gtacgagaag tcgaagcgac
    19201 cctcgaccgg ctcgacctgc tcccacgcga ccgggatctc gagcgtgttg gcgtgcaggt
    19261 cgtccagcgc gggccagacc ttgccgagca cggccgggta gttgctggaa ttgttcgcct
    19321 gcgcgcccag catcaggaac ggcgcgccgt cgacgacgag cgccgtgcgc ccgtcgcgcg
    19381 tctcgaggtg cggcgccggg ttcgcggccg gggccgcgtt cgcgccgccg acgcaggcgg
    19441 ccagcagcag gggggcgacg gccaagcggg gtgggatcgt gtacatgcca gggtcggtgc
    19501 aagtgcggtc aggcggtgga cgcgccgggc cgcgatgggg aatcgggtgg cccgtgccgc
    19561 cggatcgcgg cacgggcggc gtgctcacgt cagaacgtgt agcgcagctg gaggttgtag
    19621 ctcgggcccg aggtgaacca cgcgcgaccc atcatgccga tgtgctgctg catcagctgc
    19681 ttgtaggtcg agtcggtcag gttggtgccc tccaggccga cggtcaggtg gtcgtcgaac
    19741 ttccagaaga tgcccgcgtc cacctcgcca tagctgtccg cccaggtcgg caggccccat
    19801 gcgatgttgt gcacggtcgg attggccggg tccgtcccgt tggtgccctg ggtgccgttc
    19861 acgttgacgc cctgcaggta cttcgagcgc cagttgtatg ccacgcgcgc cgagagcgga
    19921 ccttggtcgt acagcagcgc gaagttgaac gagttgcgcg agacgccctg cagcggcagg
    19981 ttgccgaagg gttggccgtt ggtgtcgcag ccgttcaggt tcaggttgaa gttgtcggcg
    20041 ccgttgctgc tgccgcaggt cgtggcggtc accgggctgt agagcgatgt atgcgtgttg
    20101 acgaaggtgt agttcgcctg catcccgaag cccgacagga agcccggcag cttgtcgaag
    20161 tattgctggt acgccaactc gaagccgcgc gcgtagccct tggcgccgtt gaccgggccg
    20221 gtggtcacga agttgaccgt gttgcccgag gtgtcggtga cggggacgat gtagctctgg
    20281 ttgatgatga tgtccttgag ctccttgtcg aaggcggcga aggtcaggga cccggtcctg
    20341 gagaagtacc actccgccgt cgcgtccgcc tggttcgact tgatgggccg cagattcgga
    20401 ttgcccgtcg cggtgccggt gttgctcacg gacgtgatgt cggtcccggt gttcttgaag
    20461 ctcgtgctca gcggcatgta cgcctgcagc tgcgagaagt cgggccgggt catggcccgg
    20521 gcgagcgcga agcggaactg caggtcgtcc cgcggcttgg cccgcaggtt caggctgggc
    20581 aggaagtcgt tgaaggtgtt cttgaagtcc ttctcgacgg cgaacgccgt gatcgtcggg
    20641 atggtgacgc cggtgagcgt gccgctcggg atgccgctcg gcgccgtgta ggtgacgtag
    20701 ccatgggcct cggattcggt gcgcaccatc cgcaggccca ggttgccgtc gaccggcacc
    20761 ttccagtcgt cccaggcgaa gcgcagctgg ccgtacgccg tctgcgtgcg ctcgccctgg
    20821 tcgttggtgc cggcctggtt gttggcgccg taggccgcgg gcgtccagtt gccgcaggtg
    20881 tttccgctga cgcacaggag gttggtgtat ccgtgcagcg tctcgtacgt gttcgggtag
    20941 ccggcggcca gcgacgtgtc gggcatgacc agcgacggca ccgagacctt gccgttcatg
    21001 aagttcgaga agctcacgag gtgcgtgttc ccgctgaagc gcgggtcgct caggtatgcc
    21061 aggttcgtga tgtcccaacc cttctgccag gtctgcgtga tcgcggccca gttgtagctc
    21121 gggttggagt tctcggtcgt ggcgccgcgg tcggtcaggc ggatgccgaa gcgcaggtcg
    21181 ctgaggacgg gatcgtcgaa gctgtacctc gcgtcgagct tgagcgcctt ctcggtggcg
    21241 atgcccttgt cgaagtgctc catcgtgaag gcccagtagt agctggccgg attcgccagg
    21301 gcggcgaggt cggccgagtc gaagctgagc ttgggcaggc cgccagacag atccagcgtc
    21361 tccttggcca tctgcgtgcc cgtggccacg gtggagtcga agtcccgggt ggtcgccttg
    21421 accagttgca ggtcggcgtt gaagttccag cggtcgctgg cgcgccagtt cacgttccag
    21481 gagatgtcct gcgtgtcgga cttgcgcttg gccacgcggg tgtcggcgcc gaagttcagg
    21541 ccgccatcgg cgtcgtcgcg cagcgtgccg ctctggaaca cgccgccgga gctgaacttc
    21601 gcgcccgggt cgaccgtgag gttgtagggc gtggacgcct gcgtgaagat cgcgttctcg
    21661 tcccactcct ggtcgtagcg cgacttgaag tacgtcagcg aggaggagac ggtgtccttc
    21721 ttccactgca gcgccgcgta gatgccgttg cgcctgttgt tgaagtcgac gcggcgccag
    21781 gaggcgctct cgggcaccca gacggattgc ccgttggact ggacgttgaa gtacgggtcg
    21841 acctggaacg cgtcggtgcg cgtgttgctc tccgagtgcg cgaggtcgat cagcgcgccg
    21901 aactggcccg cgctgcccag atcccagcga ttggagtaca ggccggagat cttggggctc
    21961 ttcttgccgg cgagcgtcga gtagccttcc gacagcgaga tcgacacctt ctggccggtg
    22021 aagtcgaacg gcatcgccgt gcgcaggttc acgagaccgc caatgccgcc ttcgatctgc
    22081 tcggccgacg ggttcttgta gacgtccacg ccggccatca actcgggcgt gacgtcctcg
    22141 aagttcaacg agcggccgcc gttggccgag aacgcgtcgc ggccgttcag ctcggcgcgc
    22201 acgtaggtca ggccgcggat gttcacgccc gagccttcga ccgagtaatg ctcggggtcg
    22261 ttgttggaga tcaggtggcc gatggtgacg ccgacgacgc gctgcagcac ctcggtgacc
    22321 gagcggtcgg gcagcttgcc gatgtcctcg gcgacgatcg agtcgacgat ctcgtcggaa
    22381 ttctgcttga tcttctgcgc ggattgcagc gcggcgcgct ggccggtcac caccacctgg
    22441 gtgacctcct gcgcggccga cgcggcggcg gccggggcgg gtgccgagct ggccgccgcg
    22501 gcgggcgcgg gcgccgaact ggcggcgtcg gccggcacgg gcgccgattg cgccagcgcc
    22561 acaccactcc acaacagggc cgcttgcgct gccgctgctg acaacgctgt cctcttgaat
    22621 tgtctcatgt cgttctactc ttttttgata acgatggatc cccagccgat gatggtgggg
    22681 cgcggtgggc aagtcaaaga ctgccggaag tgctcgctcg ttcgggaccg cctgcggcga
    22741 gcactccgct caccgatgtc cccgcgttgt cgatggggga cgcgtcgctt ggccaggatc
    22801 cggcacgacg agcgcgcgcc ggaatcgaca ggcccggaat gcgggcgcgc gtcgcgtctg
    22861 ccaagggtgg tgggcctcct gtctcagttg ccgcagaagc ggttgacgag gttctcgagc
    22921 gcctcctggc ggcccgagac gggtgggaca tcgatgttgg cggccagcgc ccggtcggcc
    22981 agatcggcca gcgagacccg gcccgccagc accgcctgcc cgtgcgcgtc gttccagccg
    23041 gcgtagcgcg cgtcgacggc ggccttcagg cggccatcga ggatcatctg ttcggccacg
    23101 agcagggcgc gcgcggacac gtccatgccg ccgatgtgcc cgtggaacag gtcgacggcg
    23161 tcgatcgact ggcgccgcac cttggcgtcg aagttcaggc cgcccgagcc caggccgccg
    23221 gcctgcagga tgtggtacat cgccagcgcg gtgtcgggga cgttgtgcgg gaactggtcg
    23281 gtgtcccagc ccagctgcgg gtcgccgcgg ttcatgtcga tcgagcccag gatgccgagg
    23341 gcggccgcgg tggcgatctc gtgctcgaag ctgtggcccg acagcgtggc gtggttggcc
    23401 tcgatgttga ccttgacctc cttgtccagg ccgtggcgca ccaggaagcc gtagacagtg
    23461 gcgacgtcga agtcgtactg gtgcttggac ggctcgcgcg gcttgggctc gatgaggatc
    23521 gtgcccttga agccgatctt gtgcttgtgc tcgacgacga ggttcaggaa gcggcccatc
    23581 tggtccagtt cctggccgat gcgcgtgttc agcagcgtct cgtagccttc gcggccgccc
    23641 cacagcacgt agttctcgcc gcccaggcga tgcgtcgcct cgagcgcgtc gcgcacctgc
    23701 acggcggcca gcgcgaagat ggccgggtcg gggttcgtcg ccgcgcccga catgaagcgg
    23761 cgatggctga acaggttggc cgtgccccac agcagcttca tgccggtgcg ctgctggtgc
    23821 tcgcccagca cgtcggccat gcgcaggaag ttctcgcggc tctcgcgcgg ggtggcgcct
    23881 tcgggcgcga cgtcgcggtc gtggaagcag tagtacggcg cgcccagcct ggcgaagaag
    23941 tcgaacgcgg cgtcggcctt gacgcgcgcc agctccatcg gcgagccgcc ctcgaaccac
    24001 ggccgctgga acgtgccggt gccgaacgga tcgaggccgt tccagcagaa cgtgtgccag
    24061 tagcagacgg cgaagcgcag gtggtcctcc atgcgcttgc ccagcaccag gcggtccttg
    24121 tcgtaatggc ggaatgccag cgcgtcgtcg ctgtcggggc cgcggtacgt gatcggggcg
    24181 atgtccttga aatacgaagt cattgaggtt ctcggtcggg gtgatcgtgg ccgcgctcag
    24241 gccgcggcag ggccccacag gggcttgagt tggccgtaga tctggcggaa ctgctgcagg
    24301 cgcggctgga gaaccgcacg gcgcgcgggg tcgggcatga agcgcgcggc gaccgcgggc
    24361 ttggcgcagg cctcggcgac gccggcgccg gcggccatcc agcccaggcg cgcggcgccg
    24421 agtgcggcgc cggcgtccgc gccgcggtgg acgacgatct cgacgtccag cgcatcggcg
    24481 atgagctgcg cccacagcgc gctgcgggcg ccgccgccca ccagcgacag gctgcgcacc
    24541 tgcgtgcccg aggcgcgcag cgcggccagg ccgtccgcca ggccgaaggc gacgccttcc
    24601 agcacggagt acccgccgag cgcgcggccg ctctggtgcg acaggccgta gaacacgccg
    24661 ggcgcgaacg gatcgttgtg cggcgtacgt tcgccggcca ggtagggcag gaacaacggc
    24721 gcgctggcca gcgcgtcggg cgacagggcc gccacctcgg ccagcagcgc gggctcgtcg
    24781 gcggcgccgc aaaattgcgt gaaccagcgc aggcagctgg ccgccgacag catcacgctc
    24841 atctggtgcc agcgctgcgg gagcgcgtgg cagaacgcgt gcacggcaga gccggcgttg
    24901 gcgcggtgac gatcgtcgac gacgaacagc acgcccgacg tgcccagcga gatgaagccg
    24961 tcgccgggct cggtcgcccc gacgccgatc gcgctggcgg cgttgtcgcc cgcgccgccg
    25021 gcgacgacga cctcggggcc cagcccccag gcgcgcgcca gctcgggccg cagcacgccg
    25081 gacggcgcat tgctttccac cagccgcggc atgtggctgc ggtcgaggcc gcaggcggcc
    25141 agcagttcgt cggaccagtc gcgccgggcc acgtccagcc acagcgtgcc ggcggcgtcg
    25201 gatggatcgg acactttctc gccggtcagc tgcagccgca gccagtcctt gggcagcagg
    25261 acgctgcgcg cgcgcttgaa ggtctcgggc tcgtggcgcc gcacccacag cagcttgggc
    25321 gcggtgaagc cgggcatggc caggttggcc gcgatgtcgt gcagccgcgg gacgcgtgcc
    25381 gtgagctcgg cgcactcggc atggctgcgg ccgtcgttcc agaggatcgc gggacgcagc
    25441 accgcgtcgt cggcgtcgag gagcacggcg ccgtgcatct ggccggacag gccgatgcct
    25501 tgcacgcgcg cgaacttcag ggggtgcgcg gcgcgaaggc gagcgacggc gtcgttcgtc
    25561 gctcgccacc accgggccgg gtcctgctcc gaccacagcg gcttgggtcg ctccacttcc
    25621 agtggcgcgc cggacatgcc gacggcgtgg ccgtccgcat ccagcaggag cagcttgact
    25681 tcggaggtgc ccagatcgat gccgaggaac atcactggtc cccccgcacc gcgtggcgct
    25741 cgatggcggc gatcagcggc cgcgtcagcc ggtagcgcac cagtgccgcg gcgccgatgg
    25801 ccaccgcgtc ggcgccgcgc tgcgacagcc gcacgacggg cacgggcagg ctggcggcgg
    25861 cgcagtagtc gcccagggtg cgcagcgccg gctcgatgaa ggcgtcgccc aggtccacgc
    25921 cggcgccgcc cagcacgatg cacgcggggt cgtaggccgc ggccaggttc tgcagcaggg
    25981 tgcccaggtg gccgccggcg cgctcgacgg cctggacggt gtcgggatcg tgcgcggcga
    26041 ggcgcctggc gatccccgcg atcggcgcgg catgccggcg cgtcgcggcg cgcgagcgtg
    26101 cgggcagcat ggcacgcacg ccgatcagcg cctcggcgca tccgcgtcgc ccgcacgagc
    26161 agggcgggcc gtcgggctgc aggatcgtgt ggccgatctc gcccgcgaag ccgcggttgc
    26221 cgatcagcag gcggtcgccg acgatcacgc ccgcgcccac gccatgattg atgctcacat
    26281 acagcagcgg atcggacgcg gtgccggtgt cgaattcgag ttcgcccagc gcggcgacgt
    26341 ccgcctcgtt ctgcacgaac agcggcacgc ccgcgagcgc ggagtcgacg agcgcgcctt
    26401 ccagcagcgc gccgagcgcg acatcgcgcc agcccaggtt gggcgcgaag tgcaggaagc
    26461 cggtggtctc gtccacgccg ccgggaacgc cgatgccgat gccgatcacc tcgcgcgcgc
    26521 catcgagctc gcggcgcagc gcgcgcaggg ccgccgcgag gcgctcgatg caggcgggtg
    26581 cgccgcggtc ggcgccgtgc gtgacctgcg aggcggcgac gacttcgccc agcagcgacg
    26641 tcgccacgac gcgagtcgac tcgatgccga tctcggctcc cagcagcacg aggcgcgtcg
    26701 ggtcgatgaa cagcggcgtg ggccggcggc cgatgtcgcc ggtggccacg ctctcgcgct
    26761 cgagcagcca gccctcggcg atcagctcgc gcaccagcag gcccaccgtc gatttggtca
    26821 gctgcacgct cgccgccagg tcggcgcgcg acaggcccgg acgcgcgcac aggtggcgca
    26881 ccagcgtcat gcggttgatg cccttgagca ggcgctgctg gccggccgga tccgcgcgat
    26941 cggcgcgtgc ggccggcgcg ctggccgcac gcggcgaggc cgcggccgaa ggccaggggc
    27001 gggggagttc ggcgaggaag gccaagcgat ttaaccttaa attttcatga attcgcgcaa
    27061 gcgatttaac cttaaatcat tcagacttca atccgaagat gaacgacgca tccacgtcgt
    27121 tggggcagcc gatggggcag gccgggggac gggttcggaa aatattagtt tgaccaatgg
    27181 acgaaataaa gctcgtggaa acacctaggg acggatcggg cacgcgggct caaggttcgc
    27241 tcccgcgcgc agcgccatcg cgcgccgtgg tggcggcggt cttcgcaatc ggaaaaggaa
    27301 gccatgactg acaaggcctt gcgcaagatc gtgatcgtcg ggggcgggac ggcgggctgg
    27361 atggcggcgg cgccgctcgc gcggatgctg cgcggcggcg cgggcgcgcc gtgcgggatc
    27421 gtgctcgtcg aatcgcccga catcggcgcc atcggcgtgg gcgaggcgac gctgccgccg
    27481 atccgcgcct acaaccgcag cctcgggatc gacgaggccg acttcgtgcg ccgcacgcag
    27541 gcgagcttca agctgggcat cgagttccgc gactggggcc gcctcggcca ccgcttcttc
    27601 cacggcttcg gggacttcgg gccgtcgatc gacaaccgct cgtcgttcat gcactggctg
    27661 cggctcgcgc gcgccggcgg catggcgtcg tacgaggact ggtccaccgc caccgtgatg
    27721 gcgcgcgcca accgcttcac gccaccgcgc ggagaggtgc cgtcggcggc gaacggatac
    27781 tcgtacgcgt atcacttcga cgccgccctc tacgccgcct acctgcggga gtacgcgatg
    27841 cggctcggcg cgcagcgcat cgagggcacg atcgtcgacg tccggttgcg gaccaccgac
    27901 ggcttcatcg agtcgctgca actggcggac ggacgcctcg tcgacggcga cctgttcatc
    27961 gactgctcgg gctttcgcgg cctgctgatc gagggcgcgc tgcatgccgg ctacgaggac
    28021 tggagcgacc tgctgccctg cgacagcgcg ctcgccgtgc cgagcgcgcg cgccggcgcg
    28081 ctgacgccat acacgatgtc gactgccaag acggccggct ggcaatggcg catcccgctg
    28141 cagcaccgca ccggcaacgg ccacgtctac tgcagccgct tcacgagcga cgacgaggcg
    28201 gcccgcacgc tgctcgaggg cctggacacg ccggcgctcg gcgacccgcg cccgctgcgc
    28261 ttcaccaccg gccgccgccg caagtcgtgg gtgcgcaact gcgtggccct cgggctggcc
    28321 gccgggttcg tcgagccgct ggagtccacc agcctgcaac tgatcatgga cggcgtcggc
    28381 cgcctggtcg agaacttccc cgaccgcgac ttcgcgccgc gcctggccga cgaatacaac
    28441 cgccgcatcg cgctgcagta cgagagcatc cgcgacttca tcgtgatgca ctacaagctc
    28501 acggcgcgcg acgacacggc gttctggcgc cactgcgcgg cgatgcccat cccggactcg
    28561 ctgcagcacc agatcgagct gttccgctcg tcgggccgcg tggcgatcgt cgaccgcgac
    28621 ggctttgccg aaccgtcgtt catctcgatt ttgttcggcc tgggcgtcga gccgcgatcg
    28681 tgggatccgt tcatcgatcg catcgacgaa caggcgctgc gcacccacta cgcgcggctg
    28741 cgcgccgcca tcgcgggtac ggtcgacggt atgcccgatc atgcccacta catcgcccgg
    28801 aacgcccggg cggagccgcc gcgaatggcc tgcaccgcgc ctgcgcagcg aatggaacgg
    28861 cttccaacat cggccgacgc gccgaacgaa gccggcgcgg gaaatggccg catccaacgt
    28921 tgaatcgatg tcggaaacct cttccgattt aatgttaaat cacctagact gccacgaaca
    28981 ccgaacaacc ccatcgatcg acgacctatg aaattcacgg atggacagtg gctgctgcag
    29041 cccggggtca ccgcgcacta cgcggcccag gcctacgcgg tcgaggcgca cccggaccgg
    29101 ctggtggtgc tcgcgaccac gcgcccgatc cgc
//
LOCUS       DATCRO010000339         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1038732, whole genome shotgun sequence.
ACCESSION   DATCRO010000339 DATCRO010000000
VERSION     DATCRO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038732"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIN75_09200"
     CDS             <1..317
                     /locus_tag="VIN75_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974381.1"
                     /translation="PPPPPPSTTTIIVLPGDSADAAIIRRKHARHASSSSTTSDWTPP
                     PPPKDNCEKLNRDYAEATDRHDQLRGPSELTTRDQLLKKAGDDMERIRQLASASNCRL
                     KP"
     gene            652..3657
                     /locus_tag="VIN75_09205"
     CDS             652..3657
                     /locus_tag="VIN75_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8974382.1"
                     /translation="MLPRQPLGATAGHGGGPAAAIEELRGELQGWFDMSPLGQLVFDE
                     AGNVLRHNAALVQMLGPLPGQLRETAPDFQLLLGWPADLPAVAATRTQDGWLANAAGE
                     PLRVRMRVRGVAANGGSRLAGGAATRWIAMLEDRHLEDERDVARVEIQALMGTAGIGV
                     ATWDAARGWVGSVQRGARPGGPDGITAADSDPNRRQGSLMAVGRDLVEPESMAEYDRL
                     QAALRSRSRIEVRYAVRHQDSGRRWLMTRVEPAELGPQRGAFSVVTVDVTEEETARRR
                     NQDLLSELATILDVSPAGIAYLRGEGLVRCNHRFETLLGGGDYPPGKPFDAILASAGV
                     PAETVAALMEAVRDVGTLETELLLPAGTHRRQAVWCTLALRRTHETTPGAVGMVVVLA
                     DATRMKSQQSELEALSRERELMFNLSDVGIVHQRDGQIERANQAMADLVGMTPEALKG
                     SPMSRLYESETAWQHYLHLEKRDMATIGRSHGERQLRRRDGSLFWAQVSQRPVDIGRP
                     QAGTILSFVNIDDRQRARESLVQQADRTRAILDSVLVGIVTVGDTGIEWMNRSARRMF
                     GGELADFIGAPISVVAGDELDHPLRRTDYLETLEEGLAETFECRLRARDGRHFWVVGN
                     VVATGQDTAHRQLTYALLDIERRRQAEVSIVQARASLQRIIETAPLAIALFDAVSQQV
                     VQLNQMAATFAGRPMKEILGRVPSQWLPGPEGAKLAADLHRALAAPGSVQRELRRSAD
                     EGEPGGGVQRVWDIRIVSLRSAADAPDQLLMVASDVTAQRAADEARLAAAIAQREMLI
                     KEVHHRIKNNLQGVAGLLQQSASRHPEAAGAIAEAVSQVHAIAQVHGLQVGVTGPMRV
                     RGVVEAIATSVQRMFGRHITVEVSGPELLHRFALTEADSIPIALTVNELFTNAIKHSA
                     PAAGEPATDIRCRIVCGETTVMIVIANQGRLKAGFSLAGIPAGVSGLGLVRSLLPRKG
                     AKMAIEAEGDSVVARLELAPPAVALVDAA"
     gene            complement(3671..4183)
                     /locus_tag="VIN75_09210"
     CDS             complement(3671..4183)
                     /locus_tag="VIN75_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974383.1"
                     /translation="MPARSPVRFLAAALALACCGVARPGPAPKPAPADIAAFEKALPT
                     PGRDRVFLELAPDLEARLAIDPAVVRAVDPGVACDPDAFSLSMSEGNLGLAGHVQTTC
                     SPDHHSQSLLKLADGSAWITVCGAATGTQAKAWLDVQVGDRSLHASAQAPAAKLRPAH
                     DCSGWTKVSR"
     gene            4332..4805
                     /locus_tag="VIN75_09215"
     CDS             4332..4805
                     /locus_tag="VIN75_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974384.1"
                     /translation="MTRPLIAPALAAALAAAVAAVPAVAAPSPIYLVVDFKANPNKPN
                     DRRTVIVRAYENGDAGQCARGQMRYPNPPSNPKFSQCVRELPAEFAALPQGGGLPGAY
                     VLKFTVPGETEASYRLMYDMSMKDPPQICRNLADYERRWGHLSPDTRIQCWVPRG"
     gene            complement(4818..5186)
                     /locus_tag="VIN75_09220"
     CDS             complement(4818..5186)
                     /locus_tag="VIN75_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8974385.1"
                     /translation="MFDHVVFGVRDYAASKAFYLAAFAPIGMKIVQENSLGVEMSADD
                     RCSLCLFQTDEKPAHLHLALRAANRQQVDAFHRAALEAGGRDNGGPGLRPKYSANYYA
                     AFVIDPDGHNVELVCHEPQG"
     gene            complement(5245..>5788)
                     /locus_tag="VIN75_09225"
     CDS             complement(5245..>5788)
                     /locus_tag="VIN75_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974386.1"
                     /translation="VHAAELACVLCLAFGFASLAARIGPRRPAVLGACVAYVAGCLAM
                     VGAAITDGFVTGDVARYYLQAGHSADTGREMIHLCYVVVQDLAAASWFFQSAGVLAMA
                     LALLRGRGLQRAVGVLGLGTGALPPLAIVLAWPTMDTAVVVGVLCAQAAWNAAAATLL
                     LRRPAPGLPAPAPRLEPRPA"
BASE COUNT          818 a         2123 c         2140 g          707 t
ORIGIN      
        1 caccgcctcc tccgccgccg tccaccacga cgatcatcgt gctgccgggc gattcggccg
       61 acgccgccat catccggcgc aagcatgcgc ggcacgcatc gtcgtcgtcg accacgtccg
      121 actggacgcc gccgcccccg cccaaggaca actgcgagaa gctcaaccgc gactatgcgg
      181 aggccaccga ccgccacgac cagctgcgag ggcccagcga gctgaccacg cgggaccagt
      241 tgctcaagaa ggccggggac gacatggagc gcatccggca gctggcctcg gcgagcaatt
      301 gccggctcaa gccctgacga agcacatggc gggttacgcc tgcggctaac ccgcccgacg
      361 gcggcacgcc gctgaggggt ctggcctctc cccgggtcgc gccggtccgc tgccgcaagc
      421 cgggacgacc gccgcgatgc ggggacaggc ctgaccccgc tttcgatcga agccggggtc
      481 aggcctgtcc ttcatccgcg tcgaacggat ctgtgcgtcg gggatcgcga cgcggacgaa
      541 ggaaaggcca gaccccttgc ggcgcgccaa gccccgtgcc acgaagtcgc gcgtagactc
      601 cggcggcata tgtccacgac cccgcccacg cctcccaaag cgcgtccccc ggtgcttccc
      661 cgccagccgc tgggcgccac cgccggccac ggcggcggac cggcggccgc catcgaggag
      721 ctgcgcggcg aactgcaggg ctggttcgac atgtcgccgc tggggcagct tgtgttcgac
      781 gaggcgggca acgtgctgcg ccacaacgcc gccctggtgc agatgctggg cccgctgccc
      841 ggccagctgc gcgagaccgc ccccgacttc cagctgctgc tcggctggcc cgccgacctc
      901 ccggccgtcg cggccacccg cacgcaggac ggctggctcg ccaacgccgc cggcgagccg
      961 ctgcgcgtgc gcatgcgcgt gcgcggcgtg gccgccaacg gcggcagccg cctcgccgga
     1021 ggggccgcca cgcgctggat cgcgatgctg gaggatcgcc atctcgagga cgagcgcgat
     1081 gtcgcccgcg tcgagatcca ggccctgatg ggcacggccg gcatcggcgt cgccacctgg
     1141 gacgccgcgc gcggctgggt cggctcggtg cagcgcggcg cgcgcccggg cggcccggac
     1201 ggcatcaccg ccgccgacag cgatccgaat cggcgccagg gcagcctgat ggccgtcggc
     1261 cgcgacctgg tcgagcccga gtcgatggcc gagtacgaca ggctgcaggc cgcgctgcgc
     1321 tcgcgctcgc gcatcgaggt gcgctacgcc gtgcggcacc aggacagcgg ccggcgctgg
     1381 ctgatgacgc gcgtcgagcc ggccgagctg ggcccgcagc gcggcgcgtt ctcggtggtg
     1441 acggtcgacg tcaccgagga ggagaccgcg cgccgccgca accaggacct gctgtccgag
     1501 ctcgccacca tcctcgacgt cagcccggcc ggcatcgcct acctgcgcgg cgaggggctc
     1561 gtgcgctgca accaccggtt cgagacgctg ctgggcggcg gcgactaccc gccgggcaag
     1621 cccttcgacg cgatcctggc cagcgccggc gtgccggccg agaccgtcgc cgcgctgatg
     1681 gaagccgtgc gcgacgtcgg cacgctggag accgagctgc tgctgcccgc gggcacgcat
     1741 cgccgccagg cggtgtggtg cacgctggcg ctgcgccgca cgcacgagac cacgcccggc
     1801 gcggtgggca tggtggtggt gctggcggac gcgacgcgca tgaagtcgca gcagagcgag
     1861 ctcgaggcgc tgtcgcgcga gcgcgagctg atgttcaacc tgtcggacgt gggcatcgtg
     1921 caccagcgcg acggccagat cgagcgcgcc aaccaggcga tggccgacct cgtgggcatg
     1981 acgccggagg ccttgaaggg ctcgccgatg tcgcgcctgt acgagagcga gacggcctgg
     2041 cagcactacc tgcacctgga aaagcgcgac atggccacga tcggccgctc gcacggcgaa
     2101 cgccagctgc ggcggcgcga cggctcgctg ttctgggccc aggtcagcca gcggccggtg
     2161 gacatcggcc ggccgcaggc gggcaccatc ctgtcgttcg tcaacatcga cgaccgccag
     2221 cgtgcccgcg agtcgctggt gcagcaggcc gaccgcacgc gcgccatcct cgactcggtg
     2281 ctggtgggca tcgtcaccgt cggcgacacc ggcatcgagt ggatgaaccg ctcggcgcgg
     2341 cgcatgttcg ggggcgagct ggccgacttc atcggcgcgc ccatctccgt ggtggcgggc
     2401 gacgagctcg accacccgct gcggcgcacc gactacctcg agacgctgga ggagggcctg
     2461 gccgagacct tcgaatgccg gctgcgcgcg cgcgacgggc gccacttctg ggtggtcggc
     2521 aacgtcgtgg ccaccggcca ggacaccgcg caccgccagc tcacgtacgc gctgctggac
     2581 atcgagcgcc gccgccaggc cgaggtcagc atcgtgcagg cgcgcgcgag cctgcagcgc
     2641 atcatcgaga ccgcgccgct ggccatcgcg ctgttcgacg cggtcagcca gcaggtggtg
     2701 cagctgaacc agatggcggc cacgttcgcc ggccggccga tgaaggagat cctgggccgc
     2761 gtgccgtcgc agtggctgcc cgggcccgag ggcgccaagc tcgcggccga cctgcaccgc
     2821 gcgctcgccg cgcccggcag cgtgcagcgc gagctgcgcc gcagcgccga cgagggcgag
     2881 ccgggcggcg gcgtgcagcg cgtatgggac atccgcatcg tctcgctgcg ctcggccgcc
     2941 gacgcgcccg accagctgct gatggtggcc tccgacgtca ccgcccagcg cgccgccgac
     3001 gaggcgcgcc tggccgccgc catcgcgcag cgcgagatgc tgatcaagga agtgcaccac
     3061 cgcatcaaga acaacctgca gggcgtcgcg ggcctgctgc agcagagcgc gtcacgccac
     3121 cccgaggccg ccggcgccat cgccgaggcc gtgagccagg tgcacgccat cgcgcaggtg
     3181 cacggcctgc aggtgggcgt gaccgggccg atgcgcgtgc gcggcgtcgt cgaggcgatc
     3241 gccacctcgg tgcaacgcat gttcgggcgc cacatcaccg tggaggtcag cggtcccgag
     3301 ttgctgcacc gcttcgcgct gaccgaggcc gactcgatcc cgatcgcgct gaccgtcaac
     3361 gagctgttca ccaacgccat caagcacagc gcgccggccg ccggcgagcc ggccaccgac
     3421 atccgctgcc gcatcgtgtg cggcgagacg acggtgatga tcgtcatcgc caaccagggc
     3481 cggctgaagg cgggcttctc gctggccggc atcccggcag gcgtctcggg cctgggcctg
     3541 gtgcgctcgc tgctgccgcg caagggcgcg aagatggcga tcgaggccga gggcgactcg
     3601 gtggtcgcgc ggctggagct ggcgccgccg gcggtggcgc tggtcgacgc ggcctgacgc
     3661 cgcgcccgcc tcagcgcgag accttcgtcc acccgctgca gtcgtgcgcg ggccgcagct
     3721 tcgccgcggg tgcctgcgcg gaggcgtgca acgaccggtc gccgacctgg acgtcgagcc
     3781 aggccttcgc ctgcgtgccg gtggccgcgc cgcacacggt gatccacgcg ctgccgtcgg
     3841 ccagcttgag caggctctgg ctgtggtggt cgggcgagca ggtggtctgc acgtggccgg
     3901 cgaggccgag gttgccctcg gacatgctca gcgaaaaggc gtccgggtcg caggcgacgc
     3961 cggggtcgac cgcgcgcacg acggcgggat cgatcgccag gcgggcctcc aggtccggcg
     4021 ccagctcgag gaagacgcgg tcgcggcccg gcgtgggcag ggccttctcg aaggcggcga
     4081 tgtcggccgg cgcgggtttg ggcgcgggcc cggggcgcgc gacgccgcag cacgcgagcg
     4141 cgagggcggc ggcgaggaag cggacgggcg agcgagcggg catggcgcgg ccaggaggca
     4201 tgacgagtcc cgtcgattgt ggcgagccgg cgcgcgctgc catccccctt gcgggtgggc
     4261 gccgcaagca ggacgcaagc tgcgggtcgt tcccggacat gccatgtgcg ttcatcgcct
     4321 agactcgagg gatgacccgc cccttgatcg cgcccgcgct ggccgcggcg ctcgcggccg
     4381 ccgtggcggc cgtgcccgcc gtcgccgccc cgtcgcccat ctacctcgtc gtcgacttca
     4441 aggcgaaccc gaacaagccg aacgaccggc gcacggtgat cgtccgtgcg tacgagaacg
     4501 gcgacgccgg ccagtgcgcg cgcggccaga tgcgctatcc gaatccgccg tccaacccga
     4561 agttctcgca gtgcgtgcgc gagctgcccg ccgaattcgc ggcgctgccg cagggcggcg
     4621 gcctgcccgg ggcctacgtg ctgaagttca ccgtgcccgg cgagaccgag gcctcgtacc
     4681 gcctgatgta cgacatgtcg atgaaggatc cgccgcagat ctgccgcaac ctggccgact
     4741 acgagcggcg ctggggccac ctgtcgccgg acacgcgcat ccagtgctgg gtgccgcgcg
     4801 ggtgacgccg ccggggctca gccctgcggc tcgtggcaca ccagttcgac gttgtgcccg
     4861 tccggatcga tgacgaaggc cgcgtagtag ttcgcgctgt acttcgggcg caggccgggg
     4921 ccgccgttgt cgcggccacc ggcctcgagc gccgcgcggt ggaacgcgtc gacctgctgg
     4981 cggttcgcgg cgcgcagcgc caggtgcagg tgcgcgggct tctcgtcggt ctggaacagg
     5041 cacagcgagc accggtcgtc ggcgctcatc tcgacgccca gcgagttctc ctggacgatc
     5101 ttcatgccga tgggcgcgaa ggcggccaga tagaacgcct tgctcgccgc gtaatcgcgg
     5161 actccgaata cgacgtggtc gaacatctgc tctcctggaa tgggccggcc gatacgccgc
     5221 atggtatcgg agccggccgt cgggtcaggc cggccgcggc tccagtcgcg gcgccggcgc
     5281 gggcaggccc ggcgccgggc ggcgcagcag cagcgtcgcg gcggccgcgt tccaggcggc
     5341 ctgcgcgcac aggacgccca cgacgacggc ggtgtccatc gtcggccagg cgaggacgat
     5401 cgcgagcggc ggcagggcgc cggtacccag gcccaggaca ccgacggcgc gctgcaggcc
     5461 gcgtccgcgc agcagcgcga gcgccatcgc cagcacgccc gccgactgga agaaccatga
     5521 cgccgcggcg aggtcctgca ccacgacgta gcacaggtgg atcatctcgc gcccggtgtc
     5581 ggcgctgtgg ccggcctgca ggtagtagcg ggcgacgtcg ccggtgacga agccgtcggt
     5641 gatggccgcg cccaccatcg ccaggcaacc ggccacgtac gcgacgcagg cgcccagcac
     5701 ggcgggccgg cgcggcccga tgcgggcggc caggctcgcg aagccgaacg ccaggcacag
     5761 cacgcaggcc agttccgccg cgtggacg
//
LOCUS       DATCRO010000340        17903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_82482, whole genome shotgun sequence.
ACCESSION   DATCRO010000340 DATCRO010000000
VERSION     DATCRO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17903
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82482"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VIN75_09230"
     CDS             <1..643
                     /locus_tag="VIN75_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8974387.1"
                     /translation="FYSHRFDPDTPLEETMGALASAVQQGKALYVGISSYSAGKTREA
                     AAILKSMGVPLLIHQPSYSMLNRWIEDDLLDALDDTGAGCIAFSALAQGLLTDKYLSG
                     VPENSRIRREGGGSLKPEHLNAQNLEHVRALNEIARGRGQTLAQMATAWVLRDPRVTS
                     ALIGASHADQIVDLAGAMKRLDFSPDELIAIDQHAVEGGINLWQKPSTDQRPG"
     gene            701..1051
                     /locus_tag="VIN75_09235"
     CDS             701..1051
                     /locus_tag="VIN75_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY8974388.1"
                     /translation="MNAWNELSTAGRLGRRGFWLRNALVLPICLFACIGADSQVGPAA
                     GLVAAALTTLFLVSTWGRRLHDRGRSAWWLLLAAVPVLGALALIVDCAFLGPRDAAAR
                     FGARPGIVLDYRTV"
     gene            1048..1515
                     /locus_tag="VIN75_09240"
     CDS             1048..1515
                     /locus_tag="VIN75_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974389.1"
                     /translation="MTNTIDHHIVNDVTQINPVRVMAIVAPTSVEEVQEALRRTSGPV
                     SIGGGRFSMGGQTASPGSLHFDMRSMNRILSFSPGERTIRVQAGTRWCDIQKFIDPHD
                     LSVKIMQTYANFTVGGALSVNCHGRYVGLGPLVLSVRNLRIVLHDGSVVDCDR"
     gene            1567..3327
                     /locus_tag="VIN75_09245"
     CDS             1567..3327
                     /locus_tag="VIN75_09245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8974390.1"
                     /translation="MVCEVELDLAENRRVKRTHAVMPLSEYWAHFKNTVRNNPKAVFH
                     NADLYAPHFTTLRSVTWVETDEPATTATRLQPLRKSYPLHQYFMWAISETPFGKERRE
                     RLIDPLLYLRKAVHWRNYEAGYDVAELEPPAREHRTYVLQEYFVPVEKLEAFVPLMAA
                     ILQRHRVNAINVSIRHAMPDEHTALAWAPVETFAFVLYHKQRTRENARNRVGVWTREL
                     IDAVTSVGGSYYLPYQPHATIEQFHRAYPQAADFFAFKWEMDPHFRFTNSLWDKYYRA
                     WLERAQPAPAPRFTSEFHRVFGDVHLSDDFYRFLQAVFRTVPEDRLHHLIRQACQLHR
                     DEESVYRHVQREFGTVKPLLADLTHALPSLIRQKQEMTRQTLQVLGPRRQFTGYAEIG
                     SKGRYFRGLASALQIKGPRWFVDERPQSFSPPDVMERGQLAKLGAQLPLDDYAPLPEA
                     IPDQSLDFVGCYIGLHHMTVDKLGPFLASIRRVLRPGGVFIVRDHDVRDENLRAIVSL
                     AHTVFNAGLSETWETNAAELRLFEPVTTWVQRLDEAGFDDSGHRVLQDNDPTDNTLLC
                     FVRRADGMAGSRAAAKAVSA"
     gene            3324..4451
                     /locus_tag="VIN75_09250"
     CDS             3324..4451
                     /locus_tag="VIN75_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974391.1"
                     /translation="MTHAGAAARALASRVRALVGACALAGTCLGASAADLKLATEPSA
                     GVAPVAAASSPLTAPPEHRRPPDQTFLTFPEWFLVHSPAEYAHYLAGGERVSAFPLFA
                     HIGQFWQAYGAVSHEVSRFPFNGGYHLMICVIGTSTTVEYSLKGVYEHTIGRLAEATV
                     TGHEQVPEERFAAAYAQRYVDFIRVDPWYLFDFGAELRTLWSDVPAHGPNLVRRWERR
                     FLLTTELLAKSGYGQLIKLATHSIYEAPKPVTAVVLTSPPPHDPATHPDLVVLQSTGR
                     ETLATIPRYEGFTTYSRWLADNHVDFREIAGNDSEILVSLLVPIHWRPAPRARVLFEQ
                     PILTVPDRKRSVLALPITQLGALLRDVDRQPALRVEHVYDF"
     gene            complement(4498..5652)
                     /locus_tag="VIN75_09255"
     CDS             complement(4498..5652)
                     /locus_tag="VIN75_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8974392.1"
                     /translation="MHTCAVTFAYDWERLPRPPTMMQLTRRAILSLLAVPLAHACLAA
                     SPHAAAPPRAITSRGPLDAEELNNISVFKSVSPSVVNITALGLERDFFSLNVQQVPQG
                     TGTGFVWDTQGHIVTNFHVIQEAAAARVTLADHTSYRAELVGVFPDRDIAVLKIGAPA
                     SKLRPVSLGTSRDLQVGQKVYAIGNPFGLDQTLTTGIVSALDREIESVTHRTIKGAIQ
                     TDAAINPGNSGGPLLDSAGRLIGVNTAIFSPSGASAGIGFAIPVDEVNRIVPRLVRDG
                     RMVRPSLGLTGAPPELNRALGLPKGVAVVRVQRGGPAARAGLKPFTRGENGGIVDGDV
                     ITAIDGDPVETLDDMLGALERLQPGDTCTVTIWRSGATRRLAMKLAESED"
     gene            complement(5761..6348)
                     /locus_tag="VIN75_09260"
     CDS             complement(5761..6348)
                     /locus_tag="VIN75_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974393.1"
                     /translation="MNTLRTATIASALVAASLLPAASFAASQTIKHVYITWYGFNDNS
                     CNVENQHNCNTIAYPKSDGYPTKHNIATEGKGTYSDPVTFATAANDSGGNAEFKPGTI
                     IYVPLVRKYFVMEDQCAECITDWKKHHYHTDLWMGPSFEQNAGPLNACEDKLTQGDSG
                     SSGTGTIIVNPASNLPVDTTPLYKNGKCTTHIYSN"
     gene            complement(6449..7735)
                     /locus_tag="VIN75_09265"
     CDS             complement(6449..7735)
                     /locus_tag="VIN75_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974394.1"
                     /translation="MGQRYQPVFGGRRATTARPGPFRRTAIAAVAVAALAAAAFVVAR
                     RPDQTPPRLPDPPRAFLGFDPSHEADPPGAAPAAPVATHRRSSRARDAGTICGLGPMP
                     APVDGQEGSSIPPSQHVDASRRLESALVNAADPRARAAGRLLRLDALRDDASDAADAT
                     AMQTVAELAADAAQSADPAVYAAAMAACARKAGEAPAACGALNANRWSSLEPTNAAPW
                     LRLAQEARAAGDGAALAADVYQASIARRIDWHDADLALAAFQAIPSDASPLARTLDAH
                     DIARASDGWAWPAYGGLVDFCAQGADANRDQTCEAVASMLLRPEARAAESRLGARLGH
                     TLGWPASRLEALRLQRDALAQAQESAPSDEQAWDCASADRLQARILRRQRLGETGALR
                     LQLAEAGMDIDAEVARLRASRAGRVARVEAAGDATP"
     gene            complement(8034..9518)
                     /locus_tag="VIN75_09270"
     CDS             complement(8034..9518)
                     /locus_tag="VIN75_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974395.1"
                     /translation="MNTSRPTTRLCVLTACLLAAGLLTACGGGDANDDATAQPQETAA
                     GGRATILATTNATASGTTVAPYFATWAFNDHSYNTWSLMDARSKTGLKAATLAFVVSG
                     GGCNLDSGGTTIDVDMKSDIAAFQAAGGKVIVSFGGADGTYLEAKCSASQMSTLIDGL
                     IQRHALRDLDFDVEGSQVGKSSLNSVRSAAIKTLQTKYPDLNVSFTLAVMPDTGLDPT
                     GIAVLKSAVAAGVKVKVVNVMAMDYGYNGDMGQFAIDAVRATAQQLANVFPGKSNAQL
                     VAMVGITPMIGHNDDSGVFTLAHAAEVVSFAQANATPLLAFWDLQRDATGASGDQGSG
                     SNTANFQFIKEFDKAASAAPAPAPSPSPSPAPAPSCSNAVTWVDGVDYPKGTIVKFTN
                     GKYYLEVNNGTNGSDGTDPTISTWYWQPATCSAPAPAPTPAPAPSPTCSKAVTWVDGK
                     NYPKGTVVKYTNGKYYLEVNNGTNGSDGTDPTISTWYWQPTSCS"
     gene            complement(9637..11055)
                     /locus_tag="VIN75_09275"
     CDS             complement(9637..11055)
                     /locus_tag="VIN75_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974396.1"
                     /translation="MSQSAVRRRSPWPVRVALVLVGGLGCWWLVGFALTSIYDEGRSQ
                     TDDQYLALMRGPAVLIEAELQRTPRTQWPALLARMKQDFDYDVSVKPIDSVKFMPAER
                     LRVLHGDIARGDEDQVDTVHYRIAGTDQVIAMGPIWATPVKSDYLNFMSVRLLGTLTL
                     LVLLAFPLLAVWVWLAPVRRDVRALDASLADLAGGEAAGRLAPVESRLMAPLAAGVAR
                     MAACWRALMDSQRELSRAVSHELRTPVARLRFGLALLDEQARLGNERVLDSMEQSLTD
                     LEDLVECSLTYARYTQARPLLDLHERELLAWVRDELAPLASAAQARGGPSLRVGVSPD
                     EDDAPLEVMFDPSHMKFALRNLVANALRFAASTVQVTVSRERRVGEMLACIDVDDDGQ
                     GVSEADRARIFEPFVRGRQPAGGTGEGHGLGLSIASRIVEWHGGRIELTHSPLGGARF
                     RTCWPLGPRVQDGVAAPQADKN"
     gene            complement(11052..11759)
                     /locus_tag="VIN75_09280"
     CDS             complement(11052..11759)
                     /locus_tag="VIN75_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974397.1"
                     /translation="MERCASVLLVEDDAPLARLVAQYLAGHDMRVEIVHRGDDAVRAI
                     RDEPPHVVILDVGLPDRDGFSVCREIRPWFDGPIVMLTARDEDIDQIVGLELGADDYL
                     VKPVQPRLLLARLRALLRRAAAPVARAPGRDCRIDMGRFSIHNDTRAALLEGAPLELT
                     SAEFDLLWLLASHAGTILSRDDLVGQLRGISYDGLDRSIDSRVSRLRRKLGDTPTSPV
                     RIRTVRGKGYLFSRVDA"
     gene            12240..14171
                     /locus_tag="VIN75_09285"
     CDS             12240..14171
                     /locus_tag="VIN75_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY8974398.1"
                     /translation="MTIHAAAILRPLASLLVLAAGLADSTQAATYVRINQVGYESGEA
                     ARAYVMTTSPLVDETYAVRDAAGAVVACGHVGSSSGKWGRYVVHPLDFTVYGSGTYTV
                     ELSGSASAASAPFRIAPAHALYAKSLANTLSYYQTSRDGPDFIANALRSAPAHLNDTS
                     AAVYRTPRDDGNDSYTADLSPTGRRIDASGGWFDAGDYLKFVETHSYTVGVMLTGIRD
                     FPDQMGAGSATSDFTREAEFGVRWLLKMWDDSSRTLHYQVGVGIDFTTNDILSDHDFW
                     RLPQADDDATPGSAPGFAGVSQSQLQYIRHRPVFVAGPAGSRISPNLAGRLAADFALC
                     FQVFHASDPALAGRCLLAAEHVYALADTAPSGELLTTSPHDFYPESVWQDDMEWGATE
                     LAIALERGSLPAGLPEGRPSAYLSQAARWAKGYIAQGNYDSLNLYDVAGLAHFDLARA
                     LTRAADSHEVVTAAQLLSNLRTLLQANAPANDPFDGLYTWSRGDSGSHVAGLSAMASE
                     IGFLADSSHHATVARRQAASMLGANGWGVSQIIGDGTTFPDCPQHQPANLVGSHDGRK
                     PVLAGAVVEGPNASGTSGDLVAMARCGGSGTYSAFDADGAVYWDWVQSYSTNEPAIDL
                     TATSMLMFAWRMAGRPAEL"
     gene            complement(14336..14650)
                     /locus_tag="VIN75_09290"
     CDS             complement(14336..14650)
                     /locus_tag="VIN75_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974399.1"
                     /translation="MEHVLKALSLFLLATFAINVLWGLLQPWVERKPPLRLVRSFLCL
                     QGTVLLAVGGVVAFYARSSPPHLFWAGCCLTVGLVELIGGLRGSDKAVLEILFLWNEP
                     IP"
     gene            complement(14787..15386)
                     /locus_tag="VIN75_09295"
     CDS             complement(14787..15386)
                     /locus_tag="VIN75_09295"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY8974400.1"
                     /translation="MVNLDGRIGGAGRHCAAVMTTRENSMKTISFRGAALALFLTVAL
                     AGHAQATPASRASLQALFQLLHADTMVDGVYGAMQKSMSQMFAQDARQRGESPARQRI
                     EAKAMEDMMGLVRAEYNWGVMEPDMIDAYQKTFTEEEVTGMINFYSTPVGQATITKIP
                     QLTQTVLAGSQARLQALLPRIRAIAEKARADADAADAAH"
     gene            complement(15440..16171)
                     /locus_tag="VIN75_09300"
     CDS             complement(15440..16171)
                     /locus_tag="VIN75_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974401.1"
                     /translation="MSMRAIVVDDEPLARSSLRVLLSRDPAVELVAECGSGEEAVASI
                     RALRPDLVFLDIQMPEVDGFDVIERLGAWVPPAVVFVTAFDQHALRAFDAGALDYVLK
                     PFTDARLNLALARAKAQIAQARAARGAADVPARVAVRSGRGIQFVALADVAWVESADY
                     CVDLHVADRTHTLRRTMASIEAELEPAGFCRVHRTAIVNLAHVRALEAGVDGEAEVVL
                     HSGRRLPTSRRHRAALQARLGLRTG"
     gene            complement(16233..17447)
                     /locus_tag="VIN75_09305"
     CDS             complement(16233..17447)
                     /locus_tag="VIN75_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8974402.1"
                     /translation="MLPTERIGVAPHWSSIAAIWGGIGLFDATQTVVSMKAMGMHHAW
                     VELFVYQALTWLPWAVATPLVMRLSVRSPLRLRWPRGVDAAAWRPWGLHAVCCLALAS
                     GASLWMAVTEHLLNPWNPASPPQAVLRLFLLNAYAWLLPALFIYYCIVMAGRVLEANE
                     RLAAQRTASAELARQLAQAQLDALRHQVEPHFLFNALNAIAGLVREHRDELAVETIAR
                     VSDFLRHTLHGATTQEVPLEEELRFAQMYLDIQKLRFGERLSLQLDLPPALGRALVPR
                     LILQPLVENAIKHGISRRAQAGVVELQAERTPVGLVISLYNDGPAIAPRVLRETAAND
                     PVDAESAGPASAGSRRSGSIGLKNVRERLRGLYGAEAALDVANVEGRGVRITIRQPWR
                     ESPGAGADTHAA"
     gene            17559..>17903
                     /locus_tag="VIN75_09310"
     CDS             17559..>17903
                     /locus_tag="VIN75_09310"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY8974403.1"
                     /translation="MPKKTSLLAFAAAVVVAAAAPVASSRAAASDASAPPRAAAPAEA
                     AAPPLVYDGDKLRAPPDYREWIYATTGLDMSYSRVNLPGHHMFDNVFVNPAAWQAYKR
                     TGTWADGTVMVLE"
BASE COUNT         2718 a         6241 c         6199 g         2745 t
ORIGIN      
        1 cttctactcg caccgcttcg accccgacac cccgctggag gagacgatgg gcgcgctcgc
       61 cagcgccgtg cagcagggca aggcgctgta cgtgggcatc agcagctact ccgccggcaa
      121 gacgcgcgag gcggcggcga tcctgaaatc gatgggcgtg ccgctgctga tccaccagcc
      181 gtcgtacagc atgctcaacc gctggatcga ggacgacctg ctcgacgcgc tggacgacac
      241 cggcgccggc tgcatcgcgt tcagcgcgct ggcgcagggc ctgctcaccg acaaatacct
      301 gagcggggta cccgagaact cgcgcatccg gcgcgagggc ggcggctcgc tcaagcccga
      361 gcacctgaac gcgcagaacc tggagcacgt gcgggcgctc aacgagatcg cgcgcggccg
      421 cggccagacc ctggcgcaga tggccaccgc ctgggtgctg cgcgacccgc gcgtgacctc
      481 cgccctgatc ggcgccagcc acgccgacca gatcgtcgac ctcgccggcg ccatgaagcg
      541 cctcgatttc tcgccggacg aactgatcgc gatcgaccag cacgcggtcg agggcggcat
      601 caacctctgg cagaagccct cgacggacca gcgacccggg taagacctgc tccccggcgt
      661 tcgatgctgc atgagaatcg aacgccaaaa gggggagcgg atgaacgcct ggaacgagtt
      721 gtcgaccgcg ggccgcctgg gccgccgcgg cttctggctg cgcaacgcgc tggtgctgcc
      781 gatctgcctg ttcgcatgca tcggcgccga ctcgcaggtc gggccggccg cggggctcgt
      841 cgccgccgcg ctcaccaccc tgttcctcgt ctccacctgg ggccgccgcc tgcatgaccg
      901 cggccgctcc gcctggtggc tgctgctggc cgccgtgccc gtgctcggcg cgctggcgct
      961 gatcgtcgac tgcgccttcc tcggcccgcg cgacgccgcc gcccgtttcg gcgcgcgtcc
     1021 cggcatcgtc ctcgactacc gcaccgtatg acgaacacca tcgaccacca catcgtcaac
     1081 gacgtcacgc agatcaaccc ggtgcgcgtg atggccatcg tcgcgccgac ctcggtggag
     1141 gaggtgcagg aggcgctgcg ccgcaccagc gggcccgtgt cgatcggcgg cggccgcttc
     1201 agcatgggcg ggcagacggc cagccccggc agcctgcact tcgacatgcg ctcgatgaac
     1261 cgcatcctgt cgttctcgcc gggcgagcgc acgatccgcg tgcaggccgg cacgcgctgg
     1321 tgcgacatcc agaaattcat cgacccgcat gacctgtcgg tcaagatcat gcagacctac
     1381 gccaacttca cggtcggcgg cgcgctgagc gtgaactgcc acggccgcta cgtcgggctg
     1441 gggccgctgg tgctgtcggt gcgcaacctg cgcatcgtgc tgcacgacgg cagcgtggtg
     1501 gactgcgacc gctagcgcca ccccgagata ttcttcggcg cgatcggcgg ctatggcgcg
     1561 ctcggcgtgg tctgcgaggt cgagctcgac ctggcggaga acaggcgcgt caagcgcacg
     1621 catgcggtga tgccgctgtc cgagtactgg gcgcacttca agaacacggt gcgcaacaac
     1681 cccaaggcgg tgttccacaa cgccgacctc tacgcgccgc acttcacgac gctgcgctcg
     1741 gtgacctggg tcgagaccga cgagccggcc accacggcca cgcgcctgca gccgctgcgc
     1801 aagtcctacc cgctgcacca gtacttcatg tgggccatct ccgagacgcc gttcggcaag
     1861 gaacgccgcg aacgcctcat cgacccgcta ctgtacctgc gcaaggcggt gcattggcgc
     1921 aactacgagg ccggctacga cgtcgccgag ctcgagccgc cggcgcgcga gcaccgcacc
     1981 tacgtgctgc aggaatactt cgtgccggtc gagaagctgg aggcgttcgt gccgctgatg
     2041 gccgcgatcc tgcagcgcca ccgcgtcaac gcgatcaacg tgtccatccg ccacgcgatg
     2101 cctgacgagc acaccgcgct cgcctgggcg ccggtcgaga ccttcgcgtt cgtgctgtac
     2161 cacaagcagc gcacgcgcga gaacgcgcgc aaccgcgtcg gcgtgtggac gcgcgagctg
     2221 atcgacgccg tcacgtcggt gggcggcagc tactacctgc cctaccagcc gcacgccacg
     2281 atcgagcagt tccaccgcgc ctatccgcag gccgccgact tcttcgcgtt caagtgggag
     2341 atggacccgc acttccgctt caccaactcg ctgtgggaca agtactaccg cgcctggctg
     2401 gagcgcgcac agcccgcacc ggcgccgcgc ttcaccagcg agttccaccg ggtgttcggc
     2461 gacgtgcacc tgtccgacga cttctaccgc ttcctgcagg ccgtgttccg cacggtgccg
     2521 gaggatcggc tgcaccacct gatccgccag gcctgccagc tgcatcgcga cgaggagagc
     2581 gtgtaccgcc acgtgcagcg cgagttcggc accgtcaagc cgctgctggc cgacctcacg
     2641 cacgccctgc cctcgctcat caggcagaag caggagatga cgcgccagac gctgcaggtg
     2701 ctgggcccgc gccggcagtt cacgggctac gccgagatcg gctcgaaggg ccgctacttc
     2761 cgcggcctgg cgtcggcgct gcagatcaag gggccgagat ggttcgtcga cgagcggccg
     2821 cagtcgttct cgccacccga cgtcatggag cgcggccagc tcgcgaagct gggcgcgcaa
     2881 ttgccgctgg acgactacgc gccgctgccc gaggccatcc ccgaccagag cctggacttc
     2941 gtcggctgct acatcggcct gcaccacatg acggtggaca agctcgggcc gttcctcgcg
     3001 tcgatcaggc gcgtgctgcg gccgggcggc gtcttcatcg tgcgcgacca cgacgtgcgc
     3061 gacgagaacc tgcgcgccat cgtctcgctc gcacacaccg tcttcaatgc gggcctgtcc
     3121 gagacctggg agaccaacgc cgccgagctg cgcctgttcg agcccgtgac cacctgggtg
     3181 cagcgcctgg acgaggcggg cttcgacgac tcgggccacc gcgtcctgca ggacaacgac
     3241 ccgaccgaca acacgctgct gtgcttcgtg cgccgcgccg acggcatggc cggcagccgc
     3301 gcggccgcga aggcggtgtc ggcgtgacgc acgcgggggc agcggcgcgc gcgctcgcgt
     3361 cacgcgtgcg tgcgctcgtg ggggcgtgcg cgctcgcggg cacgtgcctg ggtgcgagcg
     3421 cggccgatct caagctggcc accgagccga gcgccggcgt cgcgccggtg gccgccgcgt
     3481 cgagcccgct caccgcgccg ccggagcacc gccgtccgcc cgaccagacc ttcctgacct
     3541 tcccggaatg gttcctggtg cacagcccgg ccgaatacgc gcactacctg gccggggggg
     3601 agcgcgtcag cgcgttcccg ctgttcgcgc acatcggcca gttctggcag gcctacggcg
     3661 ccgtctcgca cgaggtctcg cgcttcccgt tcaacggcgg ctatcacctg atgatctgcg
     3721 tcatcggcac cagcacgacc gtcgagtact cgctcaaggg cgtctacgag catacgatcg
     3781 gccggctcgc cgaggccacg gtgacaggtc acgagcaggt gcccgaggag cgcttcgccg
     3841 cggcctacgc gcagcgctac gtcgacttca tccgggtcga cccgtggtac ctgttcgact
     3901 tcggcgccga gctcaggacg ctgtggtcgg acgtgcccgc gcacggcccc aacctcgtgc
     3961 gccgctggga gcgccgtttc ctgctgacca ccgagctgct ggccaagagc ggctacggcc
     4021 agctcatcaa gctggccacg cactcgatct acgaggcccc caagccggtg accgcggtgg
     4081 tgctgacgtc gccgccgccg cacgatcccg ccacccaccc cgacctcgtc gtcctgcaat
     4141 ccaccggccg cgagacgctc gccacgatcc cgcgctacga gggcttcacc acgtattcac
     4201 gctggctcgc cgacaaccac gtcgactttc gcgagatcgc cggcaacgac agcgagatcc
     4261 tggtgagcct gctcgtcccg atccactggc gccccgcgcc gcgcgcccgc gtactgttcg
     4321 agcaacccat cctcaccgtc ccggaccgca agcgaagcgt cctcgccctg ccgatcacgc
     4381 aactcggcgc gctgctgcgc gatgtcgacc gccaacccgc actgcgggtc gagcacgtgt
     4441 acgacttctg agccgccgcc tgctgcccgt ttgcggccgg acggatcgca atgtcgctca
     4501 gtcttccgac tctgccagct tcatggccag ccttcgagtc gcgccgcttc tccagatggt
     4561 gacggtgcag gtgtctcctg gctgcaggcg ctccagcgcg ccgagcatgt cgtcgagcgt
     4621 ttcgacagga tcgccgtcga tggcggtgat cacgtcgccg tcgacgatgc cgccgttctc
     4681 tccgcgggta aagggcttga gcccggcgcg ggccgcgggc ccgccgcgct gcacccgcac
     4741 caccgccact cctttgggca ggccgagcgc gcggttgagt tcgggcggcg cgccagtcaa
     4801 gcccagggac ggccgcacca tgcggccgtc gcggacaagg cgtggcacga tgcggttgac
     4861 ctcatccacg ggaatcgcga aaccgatccc ggcgctcgcg cccgaagggc tgaagatggc
     4921 ggtgttgacg ccgatcaagc gccccgccga gtcgagcaac ggcccgccgg aattgccggg
     4981 attgatggct gcgtcggtct ggatcgcgcc cttgatggtg cggtgggtca ccgattcgat
     5041 ctcccgatcc agcgcgctga cgatgcctgt ggtcagcgtt tggtccagcc cgaaagggtt
     5101 gccgatggcg tagaccttct ggcccacctg caggtcacga ctcgtgccaa gtgacacggg
     5161 acgcagcttg gatgcaggtg cgccgatctt cagcaccgcg atgtcgcgat cggggaacac
     5221 gccgaccagt tccgcgcggt agcttgtatg gtcggccagc gtcacgcgcg cggcggcggc
     5281 ttcctggatc acgtggaagt tggtgacgat gtggccctgc gtatcccaca cgaagccggt
     5341 gcctgtgccc tggggaacct gctggacgtt gagcgagaag aagtcgcgct ccaggccgag
     5401 cgcagtgatg ttgacgaccg agggcgacac gctcttgaac acgctgatgt tgttcagctc
     5461 ttctgcatcc agcggcccgc gcgacgtgat ggccctgggt ggcgccgcag catggggcga
     5521 ggcggccagg caggcatggg ccaacggaac ggcgaggagg gaaaggatcg cgcggcgtgt
     5581 cagttgcatc atcgtgggag gtcgaggcaa gcgttcccaa tcataggcaa aggtcacggc
     5641 acaagtgtgc atggccttca tccatccagc cttgaaggaa ggcactcctt gacgacgcgc
     5701 ttgtccgcgc cagggaccgc acgccgccgc tgcgacggcg tgcattcggg cgctccggcc
     5761 tcagttgctg tagatgtggg tggtgcactt gccgttcttg tagagcggcg tcgtgtccac
     5821 gggcaggttg ctggccggat tgacgatgat cgtgccggtg cccgacgagc ccgagtcgcc
     5881 ctgggtcagc ttgtcctcgc acgcgttgag cgggccggcg ttctgctcga aggaggggcc
     5941 catccacagg tcggtgtggt agtggtgctt cttccagtcg gtgatgcact ccgcacactg
     6001 gtcctccatg acgaagtact tgcgcacgag gggaacgtag atgatcgtgc ccggcttgaa
     6061 ttcggcattg ccgccggagt cgttggcggc ggtcgcgaag gtcaccgggt cggaataggt
     6121 gcccttgcct tcggtggcga tgttgtgctt ggtcgggtag ccgtcgctct tggggtacgc
     6181 gatcgtgttg cagttgtgct ggttctcgac gttgcacgag ttgtcgttga agccatacca
     6241 ggtgatgtag acgtgcttga tcgtctggct ggccgcgaac gacgcggcag gcagcaggct
     6301 ggcggcaaca agggcggagg cgatcgtcgc cgtgcggagc gtgttcattt gcggtttcct
     6361 cgaatggttg gtaggtgtcc cttgacggac ggatgtcgat ctggcccgtg tgcgtggcgt
     6421 cgccggtggg cctgccggcg acgctcgatc aaggtgtcgc gtcgcccgcg gcctccacgc
     6481 gcgcgacgcg gcccgcgcgt gaggcgcgca ggcgggcgac ctcggcgtcg atgtccatgc
     6541 ccgcttcggc gagctgcagc cgcagcgcgc cggtctcgcc caggcgctgg cggcgcagga
     6601 tccgggcttg caggcgatcc gcggacgcgc agtcccaggc ctgttcgtcc gagggggccg
     6661 actcctgcgc ctgcgcgagt gcatcgcgct ggaggcgcag cgcctcgagc cgggacgccg
     6721 gccagccgag ggtgtggccg aggcgcgcac cgagccgcga ttcggcggca cgggcctcgg
     6781 ggcgaagaag catcgacgcc acggcctcgc aggtctggtc gcgattggcg tccgcgccct
     6841 gggcgcagaa atccacgagc ccgccatagg ccggccacgc ccagccgtcg ctggcgcgcg
     6901 cgatgtcgtg cgcgtccagg gtgcgcgcga gcggcgaggc gtcggacggg atggcctgga
     6961 acgcggccag cgcgagatcg gcgtcgtgcc agtcgatgcg tcgagcgatc gaggcctggt
     7021 agacgtcggc cgcgagcgcg gcgccgtccc ctgcggcgcg ggcctcctgt gccaggcgca
     7081 gccagggggc ggcgttggtg ggctccaggc tgctccaccg gttggcgttc aacgcgccgc
     7141 aggccgcggg cgcttcgccg gccttgcgcg cgcaggcggc catcgccgcg gcatagaccg
     7201 ccgggtccgc cgactgcgcc gcgtcggccg ccagctcggc caccgtctgc atggccgttg
     7261 cgtcggccgc gtccgaggcg tcatcgcgca acgcgtccag ccgcaggagt cgcccggcgg
     7321 cgcgcgcccg cggatcggcc gcgttgacga gcgcgctttc caggcggcgg ctcgcgtcga
     7381 cgtgctgcga cggcgggatg ctgctgccct cctggccgtc cacgggggca ggcatcggcc
     7441 cgaggccgca gatcgtcccg gcgtcccggg ctcgcgatga gcgccgatgg gtcgcgaccg
     7501 gcgcggcggg cgcggcgccc ggcgggtcgg cctcgtggga cggatcgaag ccgaggaacg
     7561 cgcgtggcgg atcgggcagg cgcggcggcg tctggtcggg gcgacgcgcg acgacgaagg
     7621 cggccgccgc cagcgccgcc accgccacgg ccgcgatggc cgtcctgcgg aacgggccgg
     7681 gtcgagccgt cgtcgcacgg cggccgccga acaccggttg gtatcgctgg cccatcacct
     7741 cttcctttca tctggcaagg acatcgaaga gcgcaccgca gaaaaaggga acccgggcgt
     7801 tgccgggatc cgtgcctggc cgtcaaggga cgacgcgtcg gcctggcaaa tcgtcaattt
     7861 ggctgttaac tcaaattgat tatattaagt tgaaaaataa aaagcggcct gaatggcgcc
     7921 cagtcgcagc gccctcccct gttctcggta tggggcgtcc tgcgactccg agcgaaagcg
     7981 acgctgcgcg accagcccgc ccgcggcggc agaacgccgc cgcttcggcc gtgtcagctg
     8041 cagctggtcg gctgccaata ccacgtgctg atggtcgggt cggtgccgtc gctgccgttg
     8101 gtgccgttgt tgacctccag gtagtacttg ccgttcgtgt acttgacgac cgtgcccttc
     8161 ggatagttct tgccgtcgac ccacgtgacg gccttgctgc aggtcggcga cggcgcggga
     8221 gccggggtcg gcgcgggcgc cggcgcgctg caggtggcgg gctgccagta ccaggtgctg
     8281 atggtcgggt ccgtgccgtc gctgccgttg gtgccgttgt tgacctccag gtagtacttg
     8341 ccgttcgtga acttgacgat cgtgcccttc ggatagtcca cgccgtcgac ccatgtcacg
     8401 gcgttgctgc acgacggcgc gggtgcaggc gaaggcgaag gcgacggtgc tggcgccggc
     8461 gcggcggagg cggccttgtc gaattccttg atgaactgga agttggcggt gttcgagccg
     8521 gagccctggt cgcccgacgc gccggtggcg tcgcgctgca ggtcccagaa ggcgagcagc
     8581 ggcgtcgcat tggcctgcgc gaagctgacg acctcggcgg catgcgccag cgtgaagacg
     8641 cccgaatcgt cgttgtgccc gatcatcggc gtgatgccca ccatggcgac cagctgcgcg
     8701 ttgctcttgc ccgggaacac gttggcgagc tgctgcgcgg tggcccgcac ggcgtcgatc
     8761 gcgaactggc ccatgtcgcc gttgtagccg tagtccatcg ccatcacgtt gacgaccttg
     8821 accttcacgc ccgcggcgac ggcactcttg agcacggcga tgccggtcgg gtcgagcccg
     8881 gtgtcgggca tcaccgccag cgtgaacgag acgttgaggt ccgggtactt cgtctgcagc
     8941 gtcttgatgg cggcactgcg gacgctgttg agcgatgact tgccgacctg gctgccttcg
     9001 acgtcgaagt cgagatcgcg cagcgcatgg cgctggatca gtccgtcgat cagcgtgctc
     9061 atctgcgacg cgctgcactt ggcttcgagg taggtgccgt ccgcgccgcc gaacgacacg
     9121 atgaccttgc cgcccgccgc ctggaacgcg gcgatgtcgg acttcatgtc gacgtcgatg
     9181 gtcgtgccgc cgctgtccag gttgcaaccg ccgcccgaca cgacgaacgc gagcgtcgcg
     9241 gccttcaggc cggtcttgct cctggcgtcc atcaggctcc aggtgttgta gctgtggtcg
     9301 ttgaacgccc acgtcgcgaa gtacggtgcg acggtcgtgc ccgaggccgt ggcgttcgtc
     9361 gtcgccagga tggtggcgcg gccgcccgcg gccgtctcct gcggctgcgc ggtcgcgtcg
     9421 tcgttggcgt cgccgccgcc gcaagcggtc aggagtccgg cggccagcag gcaggcggtc
     9481 aggacgcaga gtctcgtggt cgggcgtgat gtgttcaaga gcggtctcca gcgatgatgt
     9541 gtcggaaggg caccggcgcg tggcgcgccg atgatccgtc agcaaggcta tgggcacgag
     9601 ggcggcgtgt ctgtgttgga ttcgtccccg tctgtgtcag ttcttgtcag cctgcggcgc
     9661 ggcgacgccg tcctgcacgc gcggcccgag cggccagcac gtgcggaagc gcgctccgcc
     9721 gagcggcgag tgcgtgagct cgatgcggcc gccgtgccac tcgacgatgc gcgacgcgat
     9781 cgacaggccc aggccgtgcc cttcgcctgt cccgccggcg ggctggcgac cacgcacgaa
     9841 gggctcgaag atgcgcgccc ggtcggcttc ggacacgccc tggccgtcgt catcgacatc
     9901 gatgcaggcg agcatttcgc caaccctgcg ttcgcgcgac accgtcacct ggacggtcga
     9961 cgccgcgaag cgcagggcgt tggcgaccag gttgcgcagg gcgaacttca tgtgcgacgg
    10021 atcgaacatg acctcgagtg gcgcgtcgtc ctcgtcaggc gagacgccga cgcgaagcga
    10081 cggccctcct cgcgcctgcg ccgccgaggc cagcggcgcg agctcgtcgc gcacccatgc
    10141 aagcagttcg cgttcatgca ggtcgagcag gggtcgcgcc tgcgtgtagc gcgcgtacgt
    10201 gaggctgcat tcgacgaggt cctcgagatc ggtcaagctc tgctccatcg agtcgaggac
    10261 gcgttcgttg cccagccgcg cctgctcgtc gaggagcgcc aggccaaagc gcagcctcgc
    10321 caccggcgtg cgcagttcgt gcgacaccgc gcgcgacagt tcgcgctggc tgtccatcag
    10381 tgcgcgccag cacgcggcca tgcgtgccac gcccgcggcc agcggcgcca tcaggcgcga
    10441 ctccacggga gcgaggcgtc cggccgcctc gccgccggcc aggtccgcga ggctcgcgtc
    10501 gagcgcgcgc acgtcgcgtc gcaccggtgc gagccagacc cagactgcca gcagcgggaa
    10561 ggcgagcagc acgagcagcg tcagcgtccc cagcaggcgc accgacatga agttgaggta
    10621 gtccgacttc accggcgtcg cccagatcgg gcccatcgcg atgacctgat ccgtgccggc
    10681 gatgcggtag tgcacggtgt cgacctggtc ctcgtcgccg cgggcgatgt cgccgtgcag
    10741 gacccgcaag cgctcggccg gcatgaactt gacgctgtcg atcggcttga cgctgacgtc
    10801 gtagtcgaag tcctgcttca tgcgcgcgag cagcgcgggc cactgcgtcc tcggcgtgcg
    10861 ctgcaactcg gcttcgatga ggacggcggg gccgcgcatc agcgcgaggt actggtcgtc
    10921 ggtctggctc cggccctcgt cgtagatgct ggtcaacgcg aagccgacga gccaccagca
    10981 ccccaggccg ccgaccagca cgagcgccac gcggaccggc caggggctgc ggcggcggac
    11041 cgcggactgg ctcatgcgtc gacgcggctg aagaggtatc ccttgccgcg caccgtgcgg
    11101 atgcgcaccg gcgaggtcgg cgtgtcgccc agcttgcggc gcaggcgcga gacgcgcgaa
    11161 tcgatcgagc gatcgaggcc gtcgtagctg atgccgcgca gctggccgac caggtcgtcg
    11221 cgcgacagga tcgtccccgc atggctggcc aggagccaca gcaggtcgaa ctccgcgctg
    11281 gtcagctcca gcggcgcgcc ttcgagcagc gccgcacggg tgtcgttgtg gatcgagaag
    11341 cggcccatgt cgatgcggca gtcccgaccc ggggcgcgcg ccaccggagc cgcagcccgg
    11401 cgcaacagcg cgcgcagccg ggccagcagg agccgcggct gcaccggctt gacaaggtag
    11461 tcgtcggcgc cgagctcgag gccgacgatc tggtcgatat cctcgtcgcg cgccgtcagc
    11521 atgacgatcg ggccgtcgaa ccagggccgg atctcgcggc agacggagaa gccgtcacga
    11581 tcgggcaggc cgacgtcgag gatcaccacg tgcggcggct cgtcgcggat cgcgcggaca
    11641 gcgtcgtcgc cgcgatgcac gatctcgacg cgcatgtcgt gacccgccag gtactgcgcc
    11701 accaaccggg ccaggggcgc gtcatcttcc accaggagca cggaggcgca gcgttccatc
    11761 gcgatgcaat tgggcacaag ggtcacggca agcgatcgtt gatcgcgtcc tcatggtatc
    11821 gatcgggtag ggcgcgatgc ggcctggcat tcccttagcg tgcccgcatc cccgacagct
    11881 tcgcgacacg ttcgcggcgg ccgcgggaat cgatcggaac gcgcatcggc ctgatgcaca
    11941 ggggtcgatc gatgtcgatg gcggcgggcg cgcccgcgcg aggtcgcgtc ggcggcgtct
    12001 tgtctcgagc cggtcgacgc atcgtcgttc gccaggattt ccgcggagta catgacgggg
    12061 tgtggcttgc cgggcgcagg gccatggcgc tgtcgcgctg ggggtggtta ccctgatcgc
    12121 gtccttctga agatagtagt caaattgatt taagaaagat acagggtctt cacggcatcg
    12181 ccacaagccc tctggctccc ctgcccggcg ggcacccacc catccgaaag gacgacaaga
    12241 tgacgatcca cgccgctgcg atcctgcgcc ccctggccag cctcctcgtg ctggccgcgg
    12301 gcctcgccga cagcacgcag gccgcgacct acgtgcgcat caaccaggtg ggctacgagt
    12361 cgggtgaagc cgcccgcgcc tacgtcatga cgacgtcgcc cctggtggac gagacctatg
    12421 ccgtgcgcga cgccgccggc gcggtcgtgg cgtgtggcca tgtcggttcc agcagcggca
    12481 agtgggggcg ctacgtggtc catcccctcg acttcaccgt gtacgggtcc ggcacctaca
    12541 ccgtcgagct gagcggatcg gcgagcgccg cgtccgcgcc gttccggatc gccccggcgc
    12601 atgcgctgta tgcgaagtcg cttgccaaca ccttgagcta ctaccagacc tcgcgcgacg
    12661 gaccggactt catcgccaac gcgttgcgga gcgctccagc ccacctgaac gacacgagtg
    12721 cggcggtcta ccggacgccg cgggacgacg gcaacgactc gtacacggcg gacttgtcgc
    12781 ccaccggtcg ccgcatcgat gccagcggcg gatggttcga cgccggcgac tacctcaagt
    12841 tcgtcgagac ccattcgtac accgtcggcg tcatgctgac gggcatccgc gacttccccg
    12901 accagatggg cgccggctcc gcgacctccg acttcacgcg cgaagcggag ttcggcgtga
    12961 gatggctgct gaagatgtgg gacgactcgt cgcgcacgct gcactaccag gtgggcgtgg
    13021 gcatcgactt caccaccaac gacatcctca gcgaccacga tttctggcgc ctgccgcagg
    13081 cagacgacga cgcgacgccc ggatccgcgc ctggcttcgc cggcgtctcg cagtcgcagc
    13141 tgcagtacat ccgacatcgc cccgtgttcg tggccggccc ggcgggctcc cgcatcagtc
    13201 cgaacctcgc cgggcgcctg gccgcggact tcgcgttgtg cttccaggtc ttccacgcca
    13261 gcgatccggc cctggcgggc cgctgcctgc tggcggccga gcacgtctac gcgttggccg
    13321 acaccgcgcc cagcggcgaa ctcctgacga cttcgccgca tgacttctat ccggagtccg
    13381 tctggcagga cgacatggag tggggcgcca ccgagctcgc catcgcgctc gagaggggat
    13441 cgctgccggc cgggttgccc gaaggacgac cgtcggccta cctgtcccag gccgcgcgct
    13501 gggcgaaggg ctacatcgcg cagggcaact acgactcgct caacctgtac gacgtcgccg
    13561 gcctggcgca cttcgacctg gcgcgcgcgc tgacccgcgc ggccgattcg cacgaagtcg
    13621 tgacggcggc gcagctgctg tccaacctgc gcaccctgct gcaggccaac gcccctgcga
    13681 acgacccctt cgacggcctg tacacctggt ccagggggga ttccggatcg cacgtggcgg
    13741 ggctctcggc catggccagc gagatcggct tcctcgcgga ctcgagccac cacgccacgg
    13801 ttgcgcgccg acaggccgcc agcatgctgg gcgccaacgg ctggggcgtg tcccagatca
    13861 tcggggatgg gacgaccttc ccggattgcc cgcaacacca gcccgccaac ctcgtgggct
    13921 cgcacgacgg caggaagccc gtgctggccg gtgccgtcgt cgaaggcccc aatgcttccg
    13981 gtacgtcggg cgacctggtc gccatggcac gctgcggcgg cagcggcacc tacagcgcct
    14041 tcgacgccga cggcgcggtc tactgggact gggtgcagtc ctacagcacc aatgagccgg
    14101 cgatcgatct cacggccacg tcgatgctga tgttcgcgtg gcgcatggcc ggccggcctg
    14161 cagagctgta ggtatcggcc ggcggccttc gggtccgcgc aaccggcgct gcaaaggcgc
    14221 ttccggcttg cggtggccag tcagcaatgc aggccgctcg gagggcacgg gcgtggccat
    14281 tggcatgcga gagtctgcag cgtgtcgaag aacgctgcct cgggccgcac gtgcctcagg
    14341 gaatgggttc gttccaaaga aagagaatct cgagtacggc cttgtccgat ccgcgcagtc
    14401 cgccgatgag ttcgacaagg ccgacggtca ggcagcagcc tgcccaaaaa aggtgaggcg
    14461 gagacgatct tgcgtagaac gcaaccactc ctcccacggc caggaggacg gtgccctgga
    14521 ggcacaggaa actcctgacc aatctgaggg gcggcttgcg ttcgacccaa ggctggagca
    14581 gcccccacag cacgttgatg gcgaacgtgg ccagcaagaa gagggaaagg gccttcagca
    14641 catgttccat ggtccgacga gaaagcctgc gtgacggatg tggggcgatg cgagtcgaaa
    14701 tgcgcgtgta ccgaaaagct cgattgcagg tggcgatgcc gggacgtcat cgcaggccgc
    14761 cagcgggaca ccggccgcgc cgtcgctcag tgcgcggcgt cggccgcgtc ggcgtccgcc
    14821 cgcgccttct cggcgatggc gcggatgcgg ggcagcagcg cctgcaggcg cgcctgcgag
    14881 ccggccagca cggtctgcgt cagttgcggg atcttggtga tcgtcgcctg ccccaccggc
    14941 gtcgaataga agttgatcat cccggtgact tcttcctcgg tgaaggtctt ctggtaggcg
    15001 tcgatcatgt ccggctccat gacaccccag ttgtactctg cacgcacgag ccccatcatg
    15061 tcttccatcg ccttggcctc gatccgctgg cgagcgggcg attcgccacg ctgacgtgca
    15121 tcctgagcga acatctgcga catcgatttc tgcatggcgc cgtagacgcc atcgaccatc
    15181 gtgtcggcat gcagcaactg gaacaatgcc tgcagcgacg cgcggctggc cggcgtggcc
    15241 tgcgcgtgtc ccgccagcgc gacggtgagg aaaagcgcca gcgcggcgcc gcggaaactg
    15301 atcgtcttca tggagttctc cctggtggtc atcacagccg cgcagtgtcg accagcgccg
    15361 ccgattcgac catccagatt caccatgaaa gctgacggca atcttgcgcg ccggcgtcgg
    15421 cttgcccgga caggatcgcc taccccgtgc gcagccccag ccgcgcctgc aaggccgcgc
    15481 ggtgccggcg gctcgtcggc aggcgccttc cgctgtgaag cacgacctcg gcctcgccat
    15541 cgacgccggc ctccagcgca cgcacatgag cgagattgac gatggcggtg cgatggacgc
    15601 ggcagaatcc cgcgggctcc agctccgcct cgatggaggc catggtccga cgcagcgtgt
    15661 gcgtgcggtc ggccacgtgc aggtcgacgc aatagtcggc cgattcgacc caggccacgt
    15721 cggccagggc gacgaattga atgccgcggc cgctgcgcac cgcgactcgc gccggcacat
    15781 ccgccgcgcc gcgggcggcg cgcgcctgcg cgatctgcgc cttggcgcgc gccagggcga
    15841 ggttcaagcg cgcgtcggtg aaaggcttga gcacgtagtc gagcgcgccg gcgtcgaagg
    15901 cgcgcaaggc atgctggtcg aacgcggtga cgaagacgac cgcaggcggc acccacgccc
    15961 ccaggcgttc gatcacgtcg aaaccgtcga cttcgggcat ctggatgtcc aggaagacca
    16021 ggtccggcct cagcgcccgg atggaagcga ccgcctcctc gcccgagccg cattccgcca
    16081 cgagctcgac cgccgggtcg cgcgacagca gcacgcgcag cgaactgcgc gcgagcggct
    16141 cgtcgtcgac gacgatggcg cgcatgctca tgaggcaacc tccgccctgc gggctgcggt
    16201 gcgagcgccc ttcggagcgg ccgggcgggc gctcatgccg cgtgggtgtc cgcgccggcc
    16261 cccggcgact cgcgccaggg ctggcggatc gtgatgcgca cgccgcggcc ctcgacgttg
    16321 gcgacgtcca gggcggcctc ggcaccgtag aggccgcgca ggcgctcgcg cacgttcttc
    16381 agtccgatcg acccgctgcg gcgcgatccg gccgaagcgg ggccggccga ctccgcgtcg
    16441 acggggtcgt tggccgccgt ctcccgcaac acccgcggcg cgatggccgg gccatcgttg
    16501 tagagcgaga tcaccagtcc cacaggcgtg cgctcggcct gcagttccac cacgcccgcc
    16561 tgggcgcggc gcgagatgcc gtgcttgatc gcgttctcca ccagcggctg caggatcaat
    16621 cgcggcacca gcgcgcgacc cagggctggc ggcaggtcca gctgcagcga aagccgttcg
    16681 ccgaagcgca gcttctggat atccaggtac atctgcgcga agcgcaactc ctcctcgagc
    16741 ggaacctcct gcgtggtcgc gccatggagg gtgtggcgca ggaagtcgct gacgcgagcg
    16801 atggtctcga ccgcgagttc gtcacgatgc tcgcgcacca ggcccgcgat ggcgttgagc
    16861 gcgttgaaca ggaagtgcgg ctccacctga tgccgcagcg catcgagttg cgcctgcgcg
    16921 agctggcgtg cgagctccgc cgacgccgtg cgctgcgcgg ccagtcgttc gttggcttcc
    16981 agcacgcggc cggccatcac gatgcagtag tagatgaaca gcgccggaag aagccaagcg
    17041 taggcgttca gcaggaacaa gcggagcacg gcctggggcg gcgacgccgg attccacgga
    17101 ttgagcaggt gctcggtgac cgccatccac aacgacgcgc ccgaggccag ggccaggcag
    17161 cacacggcgt gcagtcccca tggacgccat gccgcggcat cgaccccgcg cggccagcgc
    17221 agtcgcaagg gtgaacgcac gctcagtcgc atcaccaggg gagtggccac cgcccagggc
    17281 agccaggtga gggcctggta gacgaacagc tcgacccacg cgtggtgcat gcccatggcc
    17341 ttcatcgaca ccaccgtctg ggtggcatcg aacagcccga tgccgcccca gatggcggcg
    17401 atcgagctcc agtgcggcgc aacgccgatt cgttcggtcg gaagcatggc gaaatggtac
    17461 gcgcaggcgc gtgccggagc gccgcccctg cagcgaatcg acgccttcgt gcagccagca
    17521 gcctcgtcca cgagcgcggc cctgcgcatc atcgcggcat gccgaagaaa acctcgctcc
    17581 tcgccttcgc cgcagcggtg gtcgtcgccg ccgccgctcc cgtggcctcg tcgcgcgccg
    17641 ccgcgtccga tgcatccgcc ccaccgcggg ccgccgcccc ggcggaggcg gccgcgccgc
    17701 cgctcgtcta cgacggcgac aagctgaggg cgccgcccga ctaccgcgaa tggatctacg
    17761 ccacgacggg cctggacatg agctacagcc gggtcaacct gcccggccac cacatgttcg
    17821 acaacgtgtt cgtcaacccg gcggcctggc aggcctacaa gcgcaccggc acgtgggcgg
    17881 acgggacggt gatggtgctc gag
//
LOCUS       DATCRO010000341         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1027543, whole genome shotgun sequence.
ACCESSION   DATCRO010000341 DATCRO010000000
VERSION     DATCRO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027543"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIN75_09315"
     CDS             complement(<1..286)
                     /locus_tag="VIN75_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8974404.1"
                     /translation="MTQPSAMPHVMNTYGRLPFALSHGRGSRVWDTEGREYLDALAGI
                     AVNTLGHAHPKLVAAIADQAGKLIHTSNYYQVPLQETLGAMLAERSGLAAA"
     gene            490..834
                     /locus_tag="VIN75_09320"
     CDS             490..834
                     /locus_tag="VIN75_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY8974405.1"
                     /translation="MNPAQPRQYFIQGQTLDGRAFRPSDWAERLAGAMSCFRPGAAKG
                     GIGSFIGYSPYCVPRLIDNVKNVIVDERLKALEPMAWDFVMNFARDNKLPVVEGTLTE
                     DGRFQATPKTAP"
     gene            complement(1031..1339)
                     /gene="rpsT"
                     /locus_tag="VIN75_09325"
     CDS             complement(1031..1339)
                     /gene="rpsT"
                     /locus_tag="VIN75_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY8974406.1"
                     /translation="MATSSKAKKRTVRLASGRKRARQDVKLNAHNSSLRSKFRTVIKN
                     VQKAVAAGDSAKAADLFKSAQSTIDSIADKGLFHKNKAARHKSRLAAKVKALAAPAAA
                     "
     gene            complement(1563..2735)
                     /locus_tag="VIN75_09330"
     CDS             complement(1563..2735)
                     /locus_tag="VIN75_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY8974407.1"
                     /translation="MTHTTIARPLPFAFALATATILAACGGGGGGTSAPPSNSGGGQN
                     CVNGPYGTTCTPTGTTFGGAGGGNYVANTLVSDSTATQALHHDTHLVNAWGLAFNPQG
                     FSWIANAGTGSSTLYDGTGVQQSLVVTLNQGAPSGIVFNGTTDFSITENGVSGAAPFV
                     FATLQGQISAWAPTVDGTHSFTIVDNSTSGAVYTGLAINTGTGGDMLYAANFAKGKVD
                     VFSGTFSPVTTTGGFTDPTLPAGYAPFGIQQIGGKVYVAYAQPDPVTHEKTGAGLGLV
                     DVFDLQGNLTQHLVGTGGALNAPWGLAMAPANFGKFSNMLLVGNFGDGKINAYDPATG
                     TLAGTVSNNDGSAIVVPGLWALQFGNGLNSQPTNTLFYTAGPGSEQHGLYGRIDMN"
     gene            complement(2819..3553)
                     /locus_tag="VIN75_09335"
     CDS             complement(2819..3553)
                     /locus_tag="VIN75_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974408.1"
                     /translation="MTAPARSNARSGAWRQGARCLLASAALALACAAHAQIAGTLAVA
                     SSNRYRGMGTGDVGPVLRASVMVDLPAGAYGGVSGLWRTRDAGLASAEAMVGWSGRVD
                     ALPPDWGWDAALQRAHYAAGGDKDFTEAMLGILGPGFTLRTWYAPHYFGGYTHAFYTE
                     LNASRAIDERWHAFGHVGWLRYGPAADYQEHMPDRVDTLAGIGLTLSRWELQLARDGI
                     VAGRARSGLDARARRAAWIASASVAF"
     gene            complement(3579..>3801)
                     /locus_tag="VIN75_09340"
     CDS             complement(3579..>3801)
                     /locus_tag="VIN75_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974409.1"
                     /translation="VSGLLAVMDQAQARADSRAALPRLVRLPGGGIDACASLFNAAQP
                     ANAGTGAAPGCRALLAVAGGSAANVAASP"
BASE COUNT          543 a         1262 c         1417 g          579 t
ORIGIN      
        1 acgcggccgc caggcccgaa cgctcggcca gcatcgcgcc gagcgtctcc tgcagcggca
       61 cctggtagta gttggaggtg tggatgagct tgccggcctg gtcggcgatg gcggccacca
      121 gcttcggatg ggcgtggccc agcgtgttga cggcgatgcc ggccagcgcg tccaggtact
      181 cgcggccctc ggtgtcccag acccggctgc cgcggccgtg cgacaacgcg aacggcaggc
      241 ggccgtaggt gttcatcaca tggggcatgg cggaaggctg ggtcatcgcg gagagctcct
      301 gtcgggctgc gggaagaaaa acggccggcg gtcgggctgc aatcgcgctc ccgacggctg
      361 ccggccaggg ggagaaacat tctaaaggcc ggcagattgc acggaggtga acctgcctcg
      421 taacccacaa gattggtgcg atgcagcagc gcaaggcaca atacgaggtt ccccgcctca
      481 ccgatctcga tgaacccggc tcaaccccgt caatacttca tccagggcca gaccctggac
      541 gggcgcgcct tccgcccgag cgactgggcg gagcgcctcg cgggcgcgat gtcgtgtttc
      601 cggcccggcg ccgccaaggg cggcatcgga tcgttcatcg gctactcgcc gtactgcgtg
      661 ccgcggctga tcgacaacgt caagaacgtg atcgtcgacg aacgcctgaa ggccctggag
      721 ccgatggcct gggacttcgt catgaacttc gcgcgcgaca acaagctgcc ggtggtcgag
      781 ggcacgctga ccgaggacgg ccgcttccag gccacgccca agacggcgcc ttgaggcgtc
      841 cggagacgac gaggccgcaa gggatgcggc ctttttttgc gctcacacgg tgccaggtct
      901 tgccaggcaa cgctctcgcc atcggtgact tgccaggcaa gacctggcac cgactggggt
      961 gaagcgcgcg tccggaaagc aaaaagggcg ccgaagcgcc ctttccacgt tcccgcccgg
     1021 gaagccgctc ttaggcggcg gcgggggcag ccagggcctt gaccttggca gccaggcggc
     1081 tcttgtggcg agcggccttg ttcttgtgga acagaccctt gtcggcgatc gagtcgatcg
     1141 tcgactgggc ggacttgaac agatcggcgg ccttggccga atcaccggcg gcgacagcct
     1201 tctggacgtt cttgatgacg gtgcggaact tcgagcgcag cgacgagttg tgcgcgttca
     1261 gcttgacgtc ttggcgagcg cgcttgcggc ccgacgcgag acggacggtg cgcttcttgg
     1321 ctttggaaga ggttgccatt tggtattagt gctccgggcg gggagaaaag tcttcgatca
     1381 taccacgggt tcggccgaaa agcacgtgcc tggcaacaaa tctgctggac gggcagacag
     1441 tgcgggggtc ggcttccggg cgacgggccc ggaaggggcc gatccggggc cggccaccgc
     1501 cagtgggcgc cggcgggctt tccagcgacg tcgcgcggcg gagccagacc ccgccggggg
     1561 catcagttca tgtcgatgcg accgtacagg ccgtgctgct cgctgcccgg gccggcggtg
     1621 tagaacagcg tgttggtcgg ctggctgttc aggccgttgc cgaactgcag cgcccacagg
     1681 cccggcacca cgatggcgga gccgtcgttg ttgctgacgg tgcctgccag cgtgccggtg
     1741 gccggatcgt aggcgttgat cttgccgtcg ccgaagttgc ccaccagcag catgttggag
     1801 aacttgccga agttggccgg cgccatcgcc aggccccagg gcgcgttgag cgcgccgccg
     1861 gtgcccacca ggtgctgcgt caggttgccc tgcaggtcga acacgtcgac caggcccagg
     1921 ccggcgccgg tcttctcgtg cgtgaccggg tcgggctgcg cgtacgcgac gtagaccttg
     1981 ccgccgatct gctggatgcc gaacggcgcg tagccggccg gcagggtcgg atccgtgaag
     2041 ccgcccgtgg tcgtgaccgg cgagaacgtg ccgctgaaca cgtcgacctt gcccttggcg
     2101 aagttggccg cgtagagcat gtcgccgccg gtgccggtgt tgatcgccag gcccgtgtag
     2161 accgcgcccg aggtgctgtt gtcgacgatc gtgaacgagt gcgtgccgtc gacggtgggg
     2221 gcccaggccg agatctgtcc ctgcagcgtg gcgaacacga atggcgccgc gccgctgacg
     2281 ccgttctcgg tgatcgagaa gtcggtcgtg ccgttgaaca cgatgccgga cggagcgccc
     2341 tggttgagcg tgaccaccag cgactgctgc acgccggtgc cgtcgtacag cgtcgagctg
     2401 cccgtgccgg cgttggcgat ccacgagaag ccctgcgggt tgaacgcgag gccccacgcg
     2461 ttgacgaggt gggtgtcgtg gtgcagcgcc tgcgtggcgg tcgagtcgct caccagcgtg
     2521 ttggccacgt agttgccgcc gccggcgccg ccgaaggtgg tgcccgtggg cgtgcaggtg
     2581 gtcccgtaag ggccgttgac gcagttctgg ccgccgccgc tgttgcttgg cggggccgag
     2641 gtgccgccgc caccgccgcc gcacgcggcc aggatcgtgg cggtggcaag cgcgaaggcg
     2701 aagggcaggg ggcgggcaat tgtggtgtgg gtcatggtct cggctcctgg gttggaatgc
     2761 gacgcggttg cagcgcctgc acgtggatgc cgaagtccgt ggccgcggtt caagcctttc
     2821 agaaggcgac gctcgcgctc gcgatccagg ctgcgcggcg cgcccgcgcg tcgagcccgc
     2881 tgcgcgcgcg gccggccacg atgccgtcgc gcgccagctg cagctcccag cgcgagagcg
     2941 tcaggccgat acccgccagc gtatcgacgc ggtcgggcat gtgttcctgg tagtcggccg
     3001 ccggcccgta gcgcagccag ccgacgtggc cgaacgcgtg ccagcgctcg tcgatggcgc
     3061 ggctggcgtt gagctcggtg tagaacgcgt gcgtgtagcc gccgaagtag tgcggcgcgt
     3121 accaggtgcg cagcgtgaag cctgggccca ggatgcccag catcgcctcg gtgaagtcct
     3181 tgtcgccgcc cgccgcgtag tgcgcgcgct gcagcgccgc gtcccagccc cagtccggcg
     3241 gcagcgcgtc gacgcggccg ctccagccga ccatcgcctc ggcgctggcc agtcccgcgt
     3301 cacgcgtgcg ccagaggccg gagacgccgc cgtacgcgcc ggccggcagg tcgaccatca
     3361 cgctggcgcg cagcacgggg ccgacgtcgc cggtgcccat gccgcggtag cggttgctgc
     3421 tggccacggc cagcgtgccc gcgatctggg catgcgccgc gcacgccagc gcgagggccg
     3481 cgctcgcgag caggcagcgc gcgccctggc gccaggcgcc ggaccgcgcg ttgctgcgtg
     3541 cgggtgccgt caatcgggcg cgcccttgca gcgatgcgtc acggcgaggc ggccacgttg
     3601 gcggcggagc cgccggcgac cgccagcagc gcgcggcagc ccggcgcggc gccggtgccg
     3661 gcgtttgccg gttgtgcggc gttgaacagc gatgcgcacg cgtcgatgcc gccgccgggc
     3721 aggcgcacga ggcgcggcag cgcggcgcgg ctgtcggcgc gcgcctgcgc ctggtccatc
     3781 accgcgagca gccccgagac c
//
LOCUS       DATCRO010000342         6772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_588992, whole genome shotgun sequence.
ACCESSION   DATCRO010000342 DATCRO010000000
VERSION     DATCRO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588992"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VIN75_09345"
     CDS             <1..711
                     /locus_tag="VIN75_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8974410.1"
                     /translation="QDGGLHYGPETLVNYEVGTKNTLFGGTVNFNNSIYYEDFKDFQF
                     SAPVTNPDGSHSLATSNAKGATVYGAESELAARVTKDDLVTLGLAYTHTKIGYLLAGS
                     NDYTLPTCPDPLISKCLNVEGHRMPHSPTGSMNLQYEHTFRFADGGQLQPRVNFHYES
                     ASYLSVFNLGPGDTQKAYSRTDLGLRYQAAKEWWVDAYVQNVEDGRIKTNAQNSFGTW
                     QSQYMPPRTFGVNFGYDF"
     gene            876..2120
                     /locus_tag="VIN75_09350"
     CDS             876..2120
                     /locus_tag="VIN75_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8974411.1"
                     /translation="MSKFLLAIVFAAAALAQAPARAVTTITLATFPDLDRAAKAALPR
                     WHALHPDIDVRIVSLQYADHHNAMTTALATGSGLPDVMAVDFKFIGKFVGSGGFEDLY
                     QPPYDARTLGTAFVPYTIAQATNAKGELGALPADIGPGTLLYRADLVAKAGLTEGDFT
                     RDWESFIAAGIKLKAATGACILASSTDIVDIVIRSDLPAGQGIYFDRDGKVLVDQPRF
                     VRAFELGRRAHQAGIDAGTTMWTNEWVAGFRSDRIAGQMMGSWLAGHLKNWLAPATAG
                     KWRSAPLPGGLFASYGGSFYAIPKHAQHKAEAWRFVRFMTADRETQLQALVQFGNFPA
                     LVAAQRDPVVDEPIAFLGGQRAGVLWRETAARVPAIPVDKYDAMATDAVRAEYQNVIA
                     RGKDIHQALADARWLIEHRARR"
     gene            2131..3102
                     /locus_tag="VIN75_09355"
     CDS             2131..3102
                     /locus_tag="VIN75_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8974412.1"
                     /translation="MDTMLPRDTVAPAPAQAPASAQARAAARRKRLRKLAPYAFISPF
                     FVLFAVFGLFPLLFSIWLSLHQWDAASGLAAMHWVGLENYRFAITDPWFSKSLLNTLW
                     FAVASGVPQHLVALPLAFFIHMSLKRSRNVVVGAYFVPYVTSSVAIALIFTTLFSKDF
                     GVVNAILPGPPIDWLGDSFWAKPAIAFVVFWRYLGWNVVLYLSALQVISGDLYEAATL
                     DGASRWQQFRHVALPLLKPMMFFAITLTLIGNLQLFEEPFILIPPDSGVATDVMTTAM
                     FMYRMAFSDGDFGTASAVSWLLFFLIAALTYANGRLFGKEGTSDVAR"
     gene            3089..3922
                     /locus_tag="VIN75_09360"
     CDS             3089..3922
                     /locus_tag="VIN75_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8974413.1"
                     /translation="MSRAERLTPARMTALLLVGAGVVLLAAPFWFTFVFATQARADIF
                     ASPPPVWFGTHLLSNLHLLLERLPFWRNLGMSLYVACMTTALNLLLCALAGYAFAVHE
                     FAGKKLLFALVVGSMMLPSFLNMIPNFMVMDFLGWIDTPRALYVPGAAGALGIFLMRQ
                     YIGSAIPRDLIDAARMDGCGELRTFWSVVMPLCGPALGTLGLITFINAWNNFVGPLVV
                     MRSVEHYTVPLALRSMQSPNNTEWGALMAGSAIAMLPLLVAFVFSARRLVAGLTAGAV
                     K"
     gene            3924..5267
                     /locus_tag="VIN75_09365"
     CDS             3924..5267
                     /locus_tag="VIN75_09365"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8974414.1"
                     /translation="MTSKEFPFPSLPRDFTLGAATSAAQIEGHAEGDGKGPSIWDAFC
                     ATPGKIKDGRDIRVACDHIHRMPQDVERMKWLGLDAYRFSMAWPRVQPAGQGAWNEKG
                     FAFYDRLIDTLLDSGIAPHATLHHWDLPQALQESVGGWQSRETAHRFADYAAEVVRRF
                     GDRLAAIATLNEPWCMATLGYERGQFAPGLRDRRAAMQVSHHLLLGHGLALQAMRAAG
                     AKPQLGIVLNHTPAVAAEKTHADLQAARILDGLDVRWYMDPLFRGAYPADAIEHLGAD
                     APRTEAGDFAHIRQPLDFLGINLYTRHWTSTRVPPIPAPAPHGVNDMGWEIVPEILTQ
                     HLVRLTRDYMPPPILITENGMANADVVAADGSVTDTPRVRYLQGHLDAVRKAIEMGAD
                     VRGYFYWSLLDNFEWDSGYAKRFGLFRVDYDTQARTPKASAHWYRDFITHHKAQK"
     gene            5370..6479
                     /gene="ugpC"
                     /locus_tag="VIN75_09370"
     CDS             5370..6479
                     /gene="ugpC"
                     /locus_tag="VIN75_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8974415.1"
                     /translation="MAELQLQGLRRTYANGVTAVDGIDLTVHDGEFMVLVGPSGCGKS
                     TTLRMIAGLEDMSAGRMSIGGVDVTDVPPAERGVAMVFQSYALYPHMTVAENMGFALK
                     IAGRPKRELDDAVRRAAASLQLEPLLDRKPRALSGGQRQRVAIGRAIVREPKVFLFDE
                     PLSNLDASLRTQMRLEMARLHERLGATIVYVTHDQVEAMTLGDRIAVFNAGRIEQVGS
                     PSEVYNRPVNRFVAGFLGSPQMNFLPATAARDAEGLALEVPGAGRLALPPDCALPLAD
                     VKALGVRPEHWHLAAGDAGDGLRANVERVEWLGDVTLVHCRLAGRDAANGGDTVSVKT
                     TQPGDAALAAGTPVRLRARVRDVMLFDAQGRALAA"
     gene            6463..>6772
                     /locus_tag="VIN75_09375"
     CDS             6463..>6772
                     /locus_tag="VIN75_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974416.1"
                     /translation="MRSRPDIAVAVRPDAPRGAWTLPLLAHGPTRAVGGDGADDGGTV
                     AWIAVGATLDVRAFVPSPIGRGWLLLEGAAGQATLASHEPDEAAVLDVLARRHGQLAV
                     E"
BASE COUNT          967 a         2471 c         2296 g         1038 t
ORIGIN      
        1 caggacggtg gcctgcacta cggccccgag acgctggtca actacgaggt cggcaccaag
       61 aacacgctgt tcggcggcac ggtcaacttc aacaactcga tctactacga agacttcaag
      121 gacttccagt tcagcgcgcc ggtgaccaac ccggacggca gccactcgct ggccacctcc
      181 aacgcgaagg gcgcgaccgt ctatggcgcg gagtccgagc tggcggcgcg cgtgaccaag
      241 gacgacctgg tcaccctcgg gctggcgtac acgcacacca agatcggcta cctgctggcc
      301 ggcagcaacg actacacgct gccgacctgc ccggatccgc tgatcagcaa gtgcctgaac
      361 gtcgaggggc atcggatgcc gcactcgccc acgggctcga tgaacctgca gtacgagcac
      421 accttccgtt tcgcggacgg cggccagctg cagccgcgcg tgaacttcca ctacgagtcg
      481 gcgagctacc tcagcgtgtt caacctcggg ccgggcgaca cgcagaaggc ctacagccgc
      541 accgacctgg gcctgcgcta tcaggcggcc aaggaatggt gggtcgacgc ctacgtgcag
      601 aacgtcgagg acgggcgcat caagacgaac gcccagaact cgttcggcac gtggcagtcg
      661 cagtacatgc cgccgcgcac gttcggcgtg aacttcggct acgacttctg agttggtggg
      721 ctgcggcgct catcagacga accttcgcct ggcggctgcg gtctgagcgc cttcgggcgg
      781 ccgtgcgtcg ctcgggcgcc gctcctttcg cacccggccc gtcgccgatc gcggcgggtc
      841 gtggtgcggc atttccgcct cgctgaccac ccaccatgtc caagttcctc ctggcgatcg
      901 tgttcgccgc ggccgccctc gcgcaggcgc cggcccgggc ggtcaccacg atcacgctcg
      961 ccaccttccc cgacctcgac cgcgccgcca aggccgcgct gccgcgctgg catgcgctgc
     1021 atccggacat cgacgtgcgc atcgtctcgc tgcagtacgc cgaccaccac aacgcgatga
     1081 ccaccgcgct ggccaccggc tcgggcctgc ccgacgtgat ggccgtcgac ttcaagttca
     1141 tcggcaagtt cgtcggctcc ggcggcttcg aggacctgta ccagcccccc tacgacgcgc
     1201 gcacgctggg caccgcgttc gtcccctaca ccatcgccca ggccaccaac gccaagggcg
     1261 aactcggcgc gctgccggcc gacatcggtc ccggcacgct gctgtaccgc gccgacctgg
     1321 tcgccaaggc gggcctgacc gagggcgact tcacgcgcga ctgggagtcg ttcatcgcag
     1381 ccgggatcaa gctcaaggcc gccacgggcg cctgcatcct cgcgagcagc accgacatcg
     1441 tcgacatcgt catccgttcc gacctgcccg cggggcaggg gatctacttc gaccgcgacg
     1501 gcaaggtgct ggtcgaccag cctcgcttcg tgcgcgcgtt cgagctgggg cgccgcgcgc
     1561 accaggccgg catcgacgcg ggcacgacga tgtggaccaa cgagtgggtc gcgggcttcc
     1621 gctccgaccg catcgccggc cagatgatgg gctcgtggct ggccggccac ctgaagaact
     1681 ggctcgcgcc ggcgaccgcc ggcaagtggc gctcggcgcc gttgcccggc ggcctgttcg
     1741 cgtcgtacgg cggctcgttc tatgccatac cgaagcacgc gcagcacaag gccgaggcct
     1801 ggcggttcgt gcgcttcatg accgccgacc gcgagacgca gctccaggcg ctggttcagt
     1861 tcggcaactt ccccgcgctg gtcgcggcgc agcgcgaccc ggtcgtcgac gagcccatcg
     1921 ccttcctcgg cggccagcgc gccggcgtcc tgtggcgcga gaccgccgcc cgagtgccgg
     1981 cgatccccgt cgacaagtac gacgcgatgg ccaccgacgc ggtgcgcgcc gagtaccaga
     2041 acgtgatcgc gcgcggcaag gacatccacc aggcgctcgc cgacgccagg tggctgatcg
     2101 agcaccgcgc ccgccgctga acgcaacgcc atggacacca tgctcccccg cgacaccgtc
     2161 gcccccgccc ccgcgcaggc gcccgcgagc gcccaggcgc gggcggccgc acgccgcaag
     2221 cgcctgcgca agctcgcgcc ctacgccttc atctcgccgt tcttcgtgct gttcgcggtg
     2281 ttcggcctgt tcccgctgct gttctcgatc tggttgtcgc tgcaccagtg ggacgcggcg
     2341 tctggcctgg ccgcgatgca ctgggtcggg ctggagaact acaggttcgc gatcaccgat
     2401 ccgtggttct cgaagtcgct gctcaacacg ctgtggttcg cggtggcctc gggcgtgccg
     2461 cagcacctgg tcgcgctgcc gctggcgttc ttcatccaca tgagcctcaa gcgcagtcgc
     2521 aacgtcgtgg tcggcgcgta cttcgtgcct tacgtgacgt ccagcgtggc gatcgcgctg
     2581 atcttcacga cgctgttctc caaggacttc ggcgtcgtca acgcgatcct cccgggcccg
     2641 ccgatcgact ggctgggcga ctccttctgg gccaagccgg ccatcgcctt cgtcgtgttc
     2701 tggcgctacc tcggctggaa cgtggtgctg tacctgtcgg cgctgcaggt gatctcgggc
     2761 gacctgtacg aggcggccac gctggacggc gcgtcgcgct ggcagcagtt ccgccacgtc
     2821 gcgctgccgc tgctcaagcc gatgatgttc tttgccatca cgttgacgct gatcggcaac
     2881 ctgcagctgt tcgaggagcc gttcatcctg atcccgccgg actcgggcgt ggccaccgac
     2941 gtcatgacga cggcgatgtt catgtaccgc atggcgttct ccgacggcga cttcggcacc
     3001 gcgtcggccg tgtcgtggct gctgttcttc ctcatcgccg cgctgacgta cgcgaacggg
     3061 cggctgttcg gcaaggaagg gacgagcgat gtcgcgcgct gaacgcctga ctccggcgcg
     3121 catgacggcg ctgctgctgg tgggcgcggg cgtcgtcctg ctggccgcgc cgttctggtt
     3181 caccttcgtg ttcgccacgc aggcgcgcgc cgacatcttc gcgtcgccgc cgccggtgtg
     3241 gttcggcacg cacctgctgt ccaacctgca cctgctgctg gagcgcctac cgttctggcg
     3301 caacctgggc atgagcctgt acgtggcgtg catgaccacc gcgctgaacc tgctgctgtg
     3361 cgcgctggcc ggctatgcgt tcgcggtgca cgagttcgcc ggcaagaagc tgctgttcgc
     3421 gctggtggtg ggctcgatga tgctgccgag cttcctcaac atgatcccca acttcatggt
     3481 gatggacttc ctcggctgga tcgacacgcc gcgcgcgctg tacgttcccg gcgccgccgg
     3541 cgcgctgggc atcttcctga tgcgccagta catcggttcg gccatcccgc gcgacctgat
     3601 cgacgcggcg cgcatggacg gctgcggcga gctgcgcacg ttctggagcg tggtgatgcc
     3661 gctgtgcggg ccggcgctcg gcacgctggg cctgatcacg ttcatcaacg cctggaacaa
     3721 cttcgtcggg ccgctggtgg tgatgcgcag cgtcgagcac tacaccgtgc cgctggcgct
     3781 gcgcagcatg cagagcccca acaacaccga gtggggtgcg ctgatggcgg gctccgccat
     3841 cgcgatgctg ccgctgctcg tcgccttcgt cttctccgca cgccgactcg tcgcgggcct
     3901 cacggccggc gccgtcaaat gacatgacct ccaaagaatt cccattcccc tccctcccgc
     3961 gcgacttcac gctcggcgcc gccaccagcg ccgcccagat cgagggccac gccgaaggcg
     4021 acggcaaggg cccgtcgatc tgggacgcgt tctgcgccac gcccggcaag atcaaggacg
     4081 gcagggacat ccgggtcgcg tgcgaccaca tccaccgcat gccgcaggac gtcgagcgca
     4141 tgaagtggct gggcctggac gcctaccgct tctcgatggc ctggccgcgc gtgcagccgg
     4201 cggggcaggg cgcgtggaac gagaagggct tcgcgttcta cgaccgtctg atcgacacgc
     4261 tgctcgacag cggcatcgcg ccgcacgcca ccctgcatca ctgggacctg ccgcaggcgc
     4321 tgcaggaatc ggtcggcgga tggcagtcgc gcgagaccgc gcaccgcttc gccgactacg
     4381 cggccgaggt cgtgcgccgc ttcggcgacc gcctcgccgc gatcgccacg ctcaacgagc
     4441 cctggtgcat ggcgacgctg ggctacgagc gcgggcaatt cgcgccgggc ctgcgcgacc
     4501 ggcgcgcggc gatgcaggtc tcgcaccacc tgctgctggg ccacggcctg gcgctgcagg
     4561 cgatgcgcgc cgccggcgcc aagccgcagc tgggcatcgt gctgaaccac acgcccgcgg
     4621 tcgccgccga gaagacgcac gccgacctgc aggccgcgcg catcctcgat ggcctggacg
     4681 tgcgctggta catggacccg ctgttccgcg gcgcctatcc ggccgacgcg atcgagcacc
     4741 tgggcgccga cgcgccgcgc accgaggccg gcgacttcgc tcacatccgc cagccgctcg
     4801 atttcctcgg catcaacctc tacacgcgcc actggacgag cacgcgcgtg ccgccgatcc
     4861 ccgcacctgc accgcacggc gtcaacgaca tgggctggga gatcgtgccc gagatcctca
     4921 cgcagcacct ggtgcgcctg acgcgcgact acatgccgcc gcccatcctc atcaccgaga
     4981 acggcatggc caacgccgac gtggtcgcgg ccgacggctc ggtcaccgac acgccgcgcg
     5041 tgcgatacct gcaggggcat ctggatgcgg tgcgcaaggc catcgagatg ggcgccgacg
     5101 tccgcggcta cttctactgg agcctgctgg acaacttcga gtgggactcg ggctacgcca
     5161 agcgcttcgg cctgttccgc gtcgactacg acacccaggc gcgcacgccc aaggcgagcg
     5221 cgcactggta ccgcgacttc atcacccatc acaaggcgca gaaatgatga ccgccactca
     5281 ctgcgttggc tgctccccga tggggccgtc cgtccgcgcc gcagcggccg agcgctcccg
     5341 gacgtgtgtc ttcgaacagg ggccgtctca tggctgaact ccaactgcag ggcctgcgcc
     5401 gcacctatgc gaacggcgtg acggcggtcg acggcatcga cctcaccgtg cacgacggcg
     5461 agttcatggt gctggtgggg ccgtcgggct gcggcaagtc cacgacgctg cgcatgatcg
     5521 cgggcctgga ggacatgagc gccggccgca tgtcgatcgg cggcgtcgac gtcaccgacg
     5581 tgccgccggc cgagcgcggc gtggcgatgg tgttccagag ctacgcgctg tatccgcaca
     5641 tgacggtggc cgagaacatg ggcttcgcgc tcaagatcgc gggacggccc aagcgcgagc
     5701 tcgacgacgc ggtgcggcgc gccgcggcgt cgttgcagct ggagccgctg ctcgatcgca
     5761 agccgcgtgc gctgtcgggc ggccagcggc agcgcgtggc gatcggacgc gcgatcgtgc
     5821 gcgagcccaa ggtgttcctg ttcgacgaac cgctgtccaa cctcgacgcc tcgctgcgca
     5881 cgcagatgcg gctggagatg gcgcgcctgc acgagcggct gggcgccacc atcgtctacg
     5941 tgacgcacga ccaggtcgag gcgatgacgc tgggcgaccg catcgccgtc ttcaacgccg
     6001 gccgcatcga gcaggtgggc tcgccgtcgg aggtctacaa ccggccggtc aaccgcttcg
     6061 tggccggctt cctcggctcg cctcagatga acttcctgcc ggccaccgcc gcgcgcgacg
     6121 cggagggcct ggcgctggag gtgcccggcg cgggccgcct cgcgctgccg cccgactgcg
     6181 cgctgccgct ggccgacgtg aaggcgctgg gcgtgcggcc cgagcactgg cacctggccg
     6241 ccggcgacgc gggcgacggg ctgcgcgcga acgtcgagcg cgtcgagtgg ctgggcgacg
     6301 tcacgctggt gcactgccgg ctcgccggcc gcgacgccgc caacggtggc gacaccgtca
     6361 gcgtgaagac gacgcagccc ggcgacgccg cgctcgccgc gggcacgccc gtgcggctgc
     6421 gggcgcgggt gcgcgacgtg atgctgttcg atgcgcaggg ccgtgcgctc gcggcctgac
     6481 atcgcggtcg cggtgcgtcc cgacgcgccg cgcggcgcat ggacgctgcc gctgctggcg
     6541 cacggcccga cgcgcgcggt gggcggcgac ggcgccgacg acggcggcac cgtcgcgtgg
     6601 atcgcggtgg gcgcgacact ggacgtgcgc gccttcgtgc cgtcgccgat cggccgcggc
     6661 tggctgctgc tcgaaggcgc cgcgggccag gcgacgctgg cctcgcacga gcccgacgag
     6721 gccgccgtgc tggacgtgct cgcgcggcgc catggccagc tggccgtcga ac
//
LOCUS       DATCRO010000343         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_926282, whole genome shotgun sequence.
ACCESSION   DATCRO010000343 DATCRO010000000
VERSION     DATCRO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926282"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VIN75_09380"
     CDS             complement(<1..266)
                     /locus_tag="VIN75_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974417.1"
                     /translation="MTTDSPALPSGAAMDRALPSTRRRWRAPAAALALLCAAGVIAWA
                     RAPRLPVATSPQLATVHAGMFRDEVALRARVEPLRSVQLDAAEAG"
     gene            368..943
                     /locus_tag="VIN75_09385"
     CDS             368..943
                     /locus_tag="VIN75_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974418.1"
                     /translation="MPNASILLLDDDPDVGLAAQLLLQRRVAPVTCLRRPAELAAAIT
                     ALRPQLLLLDLNFGPGRTDGAQGLRLLDDVQALAQPPVVVVMTAYADIDLAVQALRRG
                     AWDFITKPWDNVRLVATCREALQRAQPAAPDAPAAMPAPLAEPVRSLAAQERAAVLAA
                     MAQAEGNLSAAARLLGLSRAALYRRLDKHGL"
     gene            933..2099
                     /locus_tag="VIN75_09390"
     CDS             933..2099
                     /locus_tag="VIN75_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974419.1"
                     /translation="MASERARLLAAALALVAAGALAHASGASARAWTGAAALAAIGLA
                     VAWPLARRPAPPAPVVEAAPPPDARQLAVLQAQLEALPVAAWLADGATLRALTSRARR
                     LVAPGGVRDPAALQRLLRDAEGGGAVVLETERGSERWQLRRQSLAVDGQAQALLALVP
                     LESELEAESLLAWQQLVQVLTHEIMNSLTPIRSLSQTALALSEEPGAEPELRSALDAI
                     ARRAESLAGFVGTYRRVSQWPAPVLAPVDVRALFARLEQAVAPAWAARGGAVSFELES
                     PTLRLQADEGQLEQALLALVNNAAHATAATPQPRLWVQARQSRGGRLQIGVRDNGPGV
                     PPGLERQIFLPFFSAREGGQGIGLTVVRQLVNGMGGRVRHVRPIEGGAAFVLSF"
     gene            2435..3670
                     /locus_tag="VIN75_09395"
     CDS             2435..3670
                     /locus_tag="VIN75_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY8974420.1"
                     /translation="MNVVERAKSILLQPAQTWPVIDAEPATVQSIYKDWLIIMAAIPA
                     VCGFIGLSLVGASVFGISYREPLVGGIVKMLVSYGLSLAVAYVMSLIVDALAPSFGAT
                     KNPVGALKVVAYGSTAAYVAGVFSLLPMLSMLGVLVALYSVYLFYVGLPTVMKCPQDK
                     AVGYTAVIVVISIVLWIVIGSLFAAFGGMGMMFAGRSSSPSFSVSTPNGDITVDTAAL
                     EAAGKRMEAARARAEAAQKAGDTASAGAAIGDMMGALTGAGGTPIAPADLKAQLPETL
                     GALKRESFETTGGNAIGIASSVAKATYTGGDNQRAELSITDLGGGLGGLASVAAWANI
                     TVDKETPDGIEKTYKDGGRTIHEQYRKDGSHAEYTVILKNGVIVETQGEHIDGATLKS
                     MAAAIDLDAIEAMKRPAKT"
BASE COUNT          488 a         1371 c         1393 g          475 t
ORIGIN      
        1 cccgcctcgg cggcgtccag ctggaccgaa cgcaggggct cgacacgcgc gcgcagcgcc
       61 acctcgtcgc ggaacatgcc cgcgtgcacg gtggccagct gcggcgaggt ggccaccggc
      121 aggcgtggcg cgcgggccca cgcgatgacg ccggcggcgc agagcagcgc gagcgccgcc
      181 gccggcgcgc gccagcggcg gcgcgtcgag ggcagcgcgc ggtccatcgc ggcaccgctc
      241 gggagggcgg gggaatcggt cgtcacggga tgcactgtcg ccgcgggcgt cctgccggcg
      301 aagggggctg tccgcatccg gacacacccg tgccgcgtgc gcgcgagacg ctgcgacact
      361 ggcacccgtg cccaacgcct ccatccttct cctggacgac gaccccgacg tcggcctcgc
      421 cgcgcagctg ctgctgcagc gccgcgtcgc gcccgtcacc tgcctgcgtc gtccggccga
      481 acttgccgcc gcgatcaccg cgctgcgccc gcagctgctg ctgctcgacc tgaacttcgg
      541 ccccggccgc accgacggcg cgcagggcct gcgcctgctg gacgacgtgc aggcactggc
      601 gcagccgccc gtggtcgtgg tgatgacggc ctacgccgac atcgacctcg ccgtgcaggc
      661 gctgcgccgc ggcgcgtggg acttcatcac caagccgtgg gacaacgtgc gcctcgtcgc
      721 gacctgtcgc gaggcgctgc agcgcgcgca gcccgcggcg cccgatgcgc cggccgcgat
      781 gccggcgccg cttgccgaac ccgtgcgctc gctggccgcg caggaacgcg ccgcggtgct
      841 ggccgcgatg gcgcaggccg agggcaacct cagcgccgcg gcgcggctgc tggggttgtc
      901 gcgcgcggcc ttgtaccgcc ggctcgacaa gcatggcctc tgagcgcgcg cgcttgctgg
      961 cggccgcgct tgcgctcgtg gccgccggcg cgctcgcgca cgcgtcaggc gcgtccgcgc
     1021 gcgcatggac gggcgcggcc gcgctcgcgg cgatcggcct ggccgtcgcg tggccgctgg
     1081 cgcggcgccc tgcgccgccg gcgccggtcg tcgaggccgc gccgccgccc gacgcgcgcc
     1141 agctggccgt gctgcaggcg cagctcgagg cgctgccggt cgcggcgtgg ctggccgacg
     1201 gcgcgacgct gcgcgcgctg acgagccggg cgcgccgtct cgttgcgccc ggcggcgtgc
     1261 gcgaccccgc ggcgctgcaa cggctgctgc gcgacgccga gggcggcggc gcggtggtgc
     1321 tggagaccga gcgcggcagc gagcgctggc agctgcgccg ccagtcgctg gcggtcgacg
     1381 gccaggcgca ggcgctgctg gcgctggtgc cgctggaaag cgagctcgag gcggagtcct
     1441 tgctcgcatg gcagcagctc gtgcaggtgc tcacgcacga gatcatgaac tcgctcacgc
     1501 cgatccgcag cctgagccag acggcgctcg cattgtcgga ggagccgggc gccgagcccg
     1561 agctgcgcag cgcgctcgac gcgatcgcac gccgcgcgga gagcctggcc ggcttcgtgg
     1621 gcacctaccg gcgcgtcagc cagtggcccg cgccggtgct cgcgccggtg gacgtgcgcg
     1681 cgctgttcgc gcggctggaa caggccgtcg cgccggcctg ggccgcgcgc ggcggcgcgg
     1741 tctcgttcga gctcgagtcg ccgacgctgc gcctgcaggc cgatgaaggc cagctcgaac
     1801 aggcgctgct cgcgctggtc aacaacgcgg cccacgccac cgccgccacg ccgcagccgc
     1861 gcctgtgggt gcaggcgcgc cagagtcgcg gcggccggct gcagatcggc gtgcgcgaca
     1921 acggccccgg cgtgccgccc ggtctcgagc gccagatctt cctgccattc ttcagcgccc
     1981 gcgagggcgg ccagggcatc ggcctgaccg tggtgcgcca gctggtcaac ggcatgggcg
     2041 gccgcgtgcg ccatgtccgg cccatcgagg gcggggcggc gttcgtgctc agcttctgac
     2101 tccgtggcga cgaggcagca ggcgtggatc ctgcggctgc gcgcaggatg acacggggtg
     2161 ggcgcgcggc ggcgacgggc cgtcgggtgg aatggcgagg aggcgtcacg cgaagcgccg
     2221 gcagcgcgcc gcgccaagcg ccagccgcgc gccgcgcgaa gcgccgggcc gtcatcctgc
     2281 gcgcagcgaa gcggagtcgc aggatccacc tccgcgggcc atccatgccc cccttcaggg
     2341 gatggattcg acggcccgac tccccgatga tgccggggct cgcgcctccc gggggtggcc
     2401 gggcccccag tcgtcaatcc aacgaggaat caccatgaac gtggtcgaac gcgccaagtc
     2461 catcctgctg caacccgcgc agacgtggcc cgtgatcgat gccgagcccg cgaccgtgca
     2521 gtcgatctac aaggactggc tgatcatcat ggccgcgatc ccggcggtct gcggcttcat
     2581 cgggctgtcg ctggtaggcg cgagcgtgtt cggcatcagc taccgcgagc cgctcgtcgg
     2641 cggcatcgtg aagatgctgg tgagctatgg cctgagcctg gcggtggcct acgtcatgtc
     2701 gctgatcgtc gacgcgctgg ccccgagctt cggcgccacg aagaacccgg tcggcgcgct
     2761 caaggtggtg gcctatggct cgacggcggc ctacgtcgcc ggcgtcttca gcctgctgcc
     2821 gatgctgagc atgctgggcg tgctggtggc gctctactcg gtctacctgt tctacgtcgg
     2881 cctgccgacg gtgatgaagt gcccgcagga caaggccgtc ggctacaccg cggtcatcgt
     2941 cgtgatctcg atcgtgctgt ggatcgtcat cggcagcctc ttcgcggcgt tcggcggcat
     3001 gggcatgatg ttcgcgggcc gctcgtcgtc gcccagcttc agcgtcagca cgcccaatgg
     3061 cgacatcacc gtcgacaccg ccgccctcga ggccgccggc aagcgcatgg aggccgcgcg
     3121 tgcccgcgcc gaggccgcgc agaaggccgg cgacacggct tccgccggcg cggccatcgg
     3181 cgacatgatg ggcgcgctga ccggcgccgg gggcacgccg atcgcgccgg ccgacctcaa
     3241 ggcgcagctg cccgagacgc tgggcgccct caagcgcgaa tcgttcgaga ccaccggcgg
     3301 caacgcgatc ggcatcgcca gctcggtggc caaggccacg tacacgggcg gcgacaacca
     3361 gcgcgccgag ctgtcgatca ccgacctcgg cggcggcctg ggcggcctcg cctcggtggc
     3421 cgcatgggcc aacatcacgg tcgacaagga aacgcccgac ggcatcgaga agacgtacaa
     3481 ggacggcggc cgcacgatcc acgagcagta ccgcaaggac ggcagccatg cggagtacac
     3541 cgtgatcctg aagaacggcg tgatcgtcga gacgcagggc gagcacatcg acggcgccac
     3601 cctcaagtcc atggccgcgg ccatcgacct cgacgcgatc gaggcgatga agcgtcccgc
     3661 gaagacctga gcgaccttcc agggcggtcg ggcgccggct ccacgttgcc gtcgagcagg
     3721 cgtagag
//
LOCUS       DATCRO010000344        11312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1106481, whole genome shotgun sequence.
ACCESSION   DATCRO010000344 DATCRO010000000
VERSION     DATCRO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106481"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VIN75_09400"
     CDS             complement(<1..335)
                     /locus_tag="VIN75_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY8974421.1"
                     /translation="MTTPIRTRIAPSPTGFLHLGTARTALYSWAYARHHGGEFVLRIE
                     DTDVARSTQDSVDQIIAAMNWLGLSYDEGPVYQMQRLDRYKAVVDTMLAAGTAYLCWC
                     TPEDLDRMK"
     gene            complement(403..1920)
                     /gene="purF"
                     /locus_tag="VIN75_09405"
     CDS             complement(403..1920)
                     /gene="purF"
                     /locus_tag="VIN75_09405"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8974422.1"
                     /translation="MCGIVGVISKSPVNQLIYDSLLLLQHRGQDAAGIVTMQGTKCFM
                     HKARGMVRDVFRTRNMRALPGNVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAHALKAELVDKDRRHINTESDTEVLINILAHELSNAAAGSRLTPEVIFAAVAA
                     VHKRIKGSYAVVALIAGHGLLAFRDPFGIRPLIYGTAETPDGPSVIVASESVALEGTG
                     HQVVRDVQPGEALFIDLEGRVHAKQCAENPTLNPCMFEYVYLARPDSVIDGISVYQAR
                     LNMGETLAQRLISTMPPSDIDVVIPIPESSRPSAMQLAQKIGKPYREGFVKNRYVGRT
                     FIMPGQSVRKKSVRQKLNAIGVEFKGRNVLLVDDSIVRGTTSKEIVQMAREAGANKVY
                     MASAAPPVKYPNVYGIDMPTPEELIASGRTMEEIRDFIGADALIYQDVDAMKRVVGKL
                     NKQISNFEASCFDGRYITGDVSLEDFSTMREQRRSQQTEEDDPARSRLALQNTGD"
     gene            complement(2049..2561)
                     /locus_tag="VIN75_09410"
     CDS             complement(2049..2561)
                     /locus_tag="VIN75_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8974423.1"
                     /translation="MNLPQFDLQLGWVDIALLAVFGLSVLIGLWRGFVFEIVSLLGWL
                     VAFVIANSAGPFLAGVLPIGDEPQVRLWIAYIAVFVVILVTCTLLARMLRALVSATPL
                     SFVDHLLGGLFGAARGALICVVAATLVMLSPYATSTTWKKSHGALWLGMALEGLKPVL
                     PQSLNVHIHT"
     gene            complement(2558..3565)
                     /locus_tag="VIN75_09415"
     CDS             complement(2558..3565)
                     /locus_tag="VIN75_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8974424.1"
                     /translation="MGLLSIFSRNKQAAAKPGKPARGGRSPARQAPLANTQNPDAADA
                     LREVRARARTRLMGATVLLLVGVIGFPLLFETQPRPVPVDLPIEIPSRNAPGVVDASA
                     HAGTRGAAAAATHAASGPAPESVSYDARSSAPAAASAAASQASGSKSAFASLAAAFSP
                     GSSPSTADVADKAAYIAPSMGGKGPAKAPAKPEKADKAEKAEKADKTEKTAAAPSPAA
                     ASRPALHTAGPVLPPPPASKASPAALLAGASADKPSDAAAPAGRFVVQVGAFLEDAKV
                     REARGKVEKLGLKTYTQPVDTPTGKRIRVRIGPFATKAEADKAAARIKAEGQAAVVLA
                     L"
     gene            complement(3625..4935)
                     /gene="folC"
                     /locus_tag="VIN75_09420"
     CDS             complement(3625..4935)
                     /gene="folC"
                     /locus_tag="VIN75_09420"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY8974425.1"
                     /translation="MTQPTTLAEWLAHCERLHPKTIDMTLARTKTLIERLGLKFAVPV
                     ITVAGTNGKGSTCAMLEAIGLAAGYKVGAYIKPHLVHFEERCRVNGRMVTADDLIPHF
                     QAVEAARGDIALTYFEFTTLVIMRALAAAPVDLVILEVGLGGRLDAVNAVDPDVSVIT
                     SIDIDHVEYLGPDRESIGREKAHIARKGRPLVVGDPVPPQSIIDYATQIGAELWLIGR
                     DFNYAGDRQQWSWSGRGRRWNALGYPSLRGANQLLNASGALAAFETLRDRLPITAQAV
                     RVGLASIDLPGRFQIVPGQPALVLDVAHNPHSVAALAQNLDQMGFFKRTFAVFGAMHD
                     KDLAGALGKITHLVDDWMLTDLPLPRAAKAAELAEVLWSLTATAPSASPKPRVSTFGS
                     PAQALSAALAQAEPTDRIVVFGSFYTVGGVLKDGPPRLVARHLG"
     gene            complement(5134..6006)
                     /gene="accD"
                     /locus_tag="VIN75_09425"
     CDS             complement(5134..6006)
                     /gene="accD"
                     /locus_tag="VIN75_09425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8974426.1"
                     /translation="MSWLEKLLPPKIQQTDPSERRTIPEGLWIKCPSCETVLYKTDLE
                     QNLNVCPKCDHHLRIGARARLDGFLDPEGRFEIGQEVIPVDPLKFKDSRKYPERLQGA
                     LEATGETDALVVMGGSIMSVPAVVACFEFDFMGGSMGSVVGERFVRGVQTAVEQRVPF
                     ICFTATGGARMQEGLFSLLQMAKTNGALTLLAKAKLPYISVLTDPTMGGVSASFAFIG
                     DVVIGEPKALIGFAGPRVIENTVREKLPEGFQRSEFLLQKGAIDMIVDRRKLRPTIAR
                     LLAQLQRQSADAVA"
     gene            complement(6081..6905)
                     /gene="trpA"
                     /locus_tag="VIN75_09430"
     CDS             complement(6081..6905)
                     /gene="trpA"
                     /locus_tag="VIN75_09430"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY8974427.1"
                     /translation="MTGRIDTVFSKLKADGRSAVVPYICCGDPYADATVDVMLALAEG
                     GADVIELGVPFSDPMADGPVIQKASERALARGIGTPQVLEAVRAFRVRDAATPVVLMG
                     YANPVEAYDLAHGKGAFVRAAAEAGVDGVLVVDYPPEESLEFSRALKAAGLAPIFLLA
                     PTSTEKRMKQVAEVAAGYVYYVSLKGVTGAGHLDTDAVARAVPRIKEHVKVPVGVGFG
                     IRDAASAKAVALVSDAVVIGSALVQCLEALPRDQVADGARRFIAGIRSAVDEIRKA"
     gene            complement(6902..8224)
                     /gene="trpB"
                     /locus_tag="VIN75_09435"
     CDS             complement(6902..8224)
                     /gene="trpB"
                     /locus_tag="VIN75_09435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY8974428.1"
                     /translation="MFEYSQPDPSGHFGPYGGSFVAETLTHAIEELNAAYEHYRKDPD
                     FQREFRHELKHFVGRPSPIYHAARTSREVGGAQIWLKREDLNHTGAHKINNTIGQALL
                     ARRMGKPRIIAETGAGQHGVATATICARYGLECIVYMGAEDVKRQSPNVYRMHLLGAK
                     VVPVESGSRTLKDALNEALRDWVTNVENTFYVIGTVAGPHPYPTMVREFQSVIGEECL
                     EQMPEMAGRQPDAVVACVGGGSNAMGIFYPYIPHEGTRLIGVEAAGLGLDSGKHSASL
                     TRGSPGVLHGNRTYLLQDANGQITETHSISAGLDYPGVGPEHAYLKDIGRAEYVGITD
                     DEALAAFHHLCRTEGIIPALESSHAFAYGMKLAATMRPDQHVLVNLSGRGDKDIGTVA
                     DLSGTAFYDRPSMAGQSVKGGKAIGGAPPDARPPEGGHAAVRSTEASQ"
     gene            complement(8296..8946)
                     /locus_tag="VIN75_09440"
     CDS             complement(8296..8946)
                     /locus_tag="VIN75_09440"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY8974429.1"
                     /translation="MSHVRTRIKICGLTREQDIDAAVAAGADAIGFVLYERSPRHVPV
                     ARLAALAKCLPPFVTPVCLFVNASPDLIDEAVAAVPTALLQFHGDEGREQCEQPGRPY
                     LRAVRMAPGVDLLDCASGYASAAGLLLDAFVEGYGGGGKAFDWSQIPSGVPLPVVLSG
                     GLSPANVIDGVMRVRPWAVDVSSGVEQAKGIKDAVLIRRFCEAVREADARVANAMT"
     gene            complement(9005..9850)
                     /gene="truA"
                     /locus_tag="VIN75_09445"
     CDS             complement(9005..9850)
                     /gene="truA"
                     /locus_tag="VIN75_09445"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8974430.1"
                     /translation="MGVAYRGLNYHGWQSQPDGNTVQDKLERALAEFVGLPRGEIIPT
                     VCAGRTDAGVHALNQVVHFDAPVTREIFSWVRGTNRYLPEDIAVQWAQNVEPGFHARN
                     GARGRRYAFLVRESAVRPAIEAGLCGWVFRPLDLDTMREAAQALIGEHDFSCFRSSQC
                     QALSPVKEMRRIDINRIGDYWRFDFEASAFLHHMVRNIMGALITIGSGNQPAAYMSEL
                     LAERNREVAPPTFPPAGLYFVGPQYDQHLGIPRRTAAHDWLPRVPVELLHADDPRPLI
                     TSPRP"
     gene            complement(9990..>11312)
                     /locus_tag="VIN75_09450"
     CDS             complement(9990..>11312)
                     /locus_tag="VIN75_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY8974431.1"
                     /translation="VVASAPAPAMVASTPAPAPVVAPPAPVRASAPAPAPVAPAPAAD
                     DSFLSSLMDPPYIVPGIAGLAVLLGGFLFWRKRQGGDRNKFSGETSFIESKIQPDSFF
                     GASGGQRVDTRDGAANSASSSLNYSLSQLDAIGDVDPVAEADVYLAYGRDLQAEEILK
                     EAQRSDPDRLAIRTKLLEVYAKRADLKTFEAQARQLQGLTNGQGEEWAKAQELGRQID
                     PTNPLYTEGGEYAIVDTDPMPLNPLGEDSDEVTLMRPPAKGGAHDTDLPIDFDSDEPP
                     TVQPGDTQQPTRPMPTEEHDGDTTRQPHSVSADMPFEVSDFDLEPGHVEVPAIPPAPA
                     PAPVHAHAPAPAAAGAPSIDFDFGDLSLDLDKPGHGGAAVAEESVLPELDLGGGGETE
                     PMARKIELADEFRRIGDHEGARELLEEVVSKADGPLKSRAQTMLDALG"
BASE COUNT         1614 a         3846 c         4119 g         1733 t
ORIGIN      
        1 tccttcatgc ggtcgaggtc ttccggcgtg caccagcaaa ggtaggccgt gccggccgcg
       61 agcatcgtgt cgaccaccgc cttgtagcga tcgaggcgct gcatctggta gaccgggccc
      121 tcgtcgtagg acaggcccag ccagttcatc gccgcgatga tctggtcgac cgagtcctgc
      181 gtcgagcgcg cgacgtcggt gtcctcgatg cgcagcacga actcgccgcc gtggtggcgc
      241 gcgtaggccc acgagtacag cgccgtgcgg gcggtgccga ggtggaggaa gcccgtcggc
      301 gacggggcga tgcgggtgcg gatgggcgta gtcacgatgt ctgaaacgag aaggcgcccg
      361 aaggcgccgt gcaggggacg tcagcggcga ttgtcggcga actcagtcgc cggtgttctg
      421 cagcgccagg cgcgagcggg ccgggtcgtc ttcctcggtc tgctgcgagc gacgctgctc
      481 gcgcatcgtc gagaaatcct cgaggctgac atcgccggtg atgtagcgcc cgtcgaagca
      541 ggacgcctcg aagttcgaga tctgcttgtt gagcttgccg accacgcgct tcatcgcgtc
      601 gacgtcctgg tagatcagcg cgtcggcgcc gatgaagtcg cggatctcct ccatcgtgcg
      661 gcccgaggcg atcagttcct cgggcgtggg catgtcgatg ccgtacacgt tcgggtactt
      721 gaccggcggc gcggccgagg ccatgtagac cttgttggcg ccggcctcgc gcgccatctg
      781 cacgatctcc ttggacgtcg tgccgcgcac gatggagtcg tccaccagca gcacgttgcg
      841 gcccttgaac tcgacgccga tcgcgttgag cttctggcgc acggacttct tgcgcaccga
      901 ctgccccggc atgatgaagg tgcggccgac atagcggttc ttgacgaagc cctcgcggta
      961 gggcttgccg atcttctgcg cgagctgcat cgccgacggg cggctcgatt cggggatggg
     1021 gatgacgacg tcgatgtccg acggcggcat cgtggagatc aggcgctgcg ccagcgtctc
     1081 gcccatgttc agccgcgcct ggtagaccga gatgccgtcg atgacggagt ccgggcgtgc
     1141 caggtacacg tactcgaaca tgcacgggtt gagcgtcggg ttctcggcgc actgcttggc
     1201 gtgcacccgg ccctcgaggt cgatgaacag cgcctcgccg ggctgcacgt cgcgcaccac
     1261 ctggtggccc gtgccctcca gcgccacgct ctcgctggcg acgatcacgc tggggccgtc
     1321 cggcgtctcg gcggtgccgt agatcagcgg gcggatgccg aacggatcgc ggaacgcgag
     1381 caggccgtgg ccggcgatca gcgcgacgac cgcatacgag cccttgatgc gcttgtgcac
     1441 ggcggcgacg gccgcgaaga tcacctcggg cgtcagcctg gagccggcgg cggcgttgga
     1501 cagctcgtgc gcgaggatgt tgatcagcac ctcggtgtcg gactccgtgt tgatgtggcg
     1561 gcgatccttg tcgaccagtt cggccttgag cgcatgggcg ttcgtgaggt tgccgttgtg
     1621 caccagcacg atgccgaacg gcgcgttgac gtagaacggc tgcgcctcct cctcgctgta
     1681 ggcgttgccc gccgtcgggt agcgcacctg gcccaggccc acgttgcccg gcagcgcgcg
     1741 catgttgcgg gtgcggaaca cgtcgcgcac catgccgcgg gccttgtgca tgaagcactt
     1801 ggtgccctgc atcgtcacga tgccggcggc gtcctgcccg cgatgctgca acagcagcag
     1861 cgagtcatag atcaactggt tgaccgggga tttggagatc acgccgacga tgccgcacat
     1921 ggtgggagtc cgtttcctga aataccgaga ggggaagaaa ttcttgtcgg ggagcgcccg
     1981 gcgctgcact ggcgccaggg acataaccgt agccggaaac cggccggaga ggtgaccgtc
     2041 ctacagggct acgtgtggat gtgcacgtta agtgactgcg gaaggacggg tttcaaccct
     2101 tccagcgcca tgcccaacca cagcgcgcca tgcgattttt tccacgtcgt ggaagtcgca
     2161 tagggcgaca gcatcaccag cgtcgccgcg accacgcaga tgagcgcgcc gcgcgccgcg
     2221 ccgaacagtc cgccgagcag gtggtcgacg aacgacagcg gcgtggccga gaccagcgcc
     2281 cgcagcatgc gcgccagcaa ggtgcacgtg accaggatga cgacgaacac ggcgatgtag
     2341 gcgatccaca gccggacctg cggctcgtcg ccgatcggca ggaccccggc caggaacggc
     2401 ccggcggagt tggcgatcac gaacgccacc agccagccca gcagggacac gatctcgaac
     2461 acgaagccgc gccacaggcc gatcagcacc gacaggccga acaccgccag cagggcgatg
     2521 tcgacccagc cgagctgcag gtcgaactgc ggcaggttca cagcgcgagc accacggcgg
     2581 cctggccctc ggccttgatc cgggcggcgg ccttgtcggc ctcggccttg gtggcgaagg
     2641 gcccgatgcg cacgcggatg cgcttgccgg tgggcgtgtc caccggctgg gtgtaggtct
     2701 tcaggcccag cttctcgacc ttgccgcgcg cctcgcgcac cttggcgtcc tcgaggaagg
     2761 cgccgacctg cacgacgaag cggccggcgg gggcggcggc atccgacggc ttgtcggcgg
     2821 acgcaccggc cagcagcgcg gcgggcgacg ccttggaggc cggcggcggc ggcagcaccg
     2881 ggccggcggt gtgcagtgcg ggcctggagg cggccgccgg cgacggcgcg gcggcggtct
     2941 tctcggtctt gtccgccttc tccgccttct ccgccttgtc ggccttctcc ggcttggccg
     3001 gcgccttggc cgggcccttg ccgcccatcg acggcgcgat gtaggcggcc ttgtccgcca
     3061 catcggcggt gctgggcgac gaaccggggg agaacgcggc agccagcgaa gcgaatgccg
     3121 acttgctgcc ggacgcctgc gaggcggccg cggatgccgc cgccggcgcg gacgaacgcg
     3181 cgtcgtacga gacggactcg ggcgccgggc cggacgcggc atgcgtcgcg gcggcggctg
     3241 cgccgcgtgt gcccgcgtgc gcggacgcgt cgaccacgcc cggcgcgttg cgcgagggga
     3301 tctcgatggg caggtcgacc ggcaccgggc gcggctgcgt ctcgaaaagc aacgggaagc
     3361 cgatgacgcc caccagcagc agcaccgtcg cgcccatcag gcgcgtgcgg gcgcgcgcgc
     3421 gcacctcgcg cagcgcgtcg gcggcatcag ggttctgggt gttggcgagc ggggcctggc
     3481 gggctggcga tcggccgccc cgcgccggtt tcccgggctt ggcggcagcc tgcttgttgc
     3541 gggagaaaat cgacagcagc cccatgcctt ctcatcggag tccggggccg gacaggtggt
     3601 gtcggcgcgg cgggcgccgc ggcttcaacc gagatgtcgg gccaccaggc gcggggggcc
     3661 gtccttcagc acgccgccca cggtgtagaa cgagccgaag accacgattc tatcagtggg
     3721 ctccgcctgg gcgagcgccg ccgacaacgc ttgggcgggc gacccgaacg tgctcacgcg
     3781 cggcttgggc gacgcggacg gcgccgtcgc ggtcagcgac cacagcacct cggccagctc
     3841 cgcggccttg gccgcgcgcg gcagcggcag gtcggtgagc atccagtcgt cgaccaggtg
     3901 cgtgatcttg cccagcgcgc cggccaggtc cttgtcgtgc atcgcgccga acacggcgaa
     3961 ggtgcgcttg aagaagccca tctggtccag gttctgcgcc agcgccgcca ccgagtgcgg
     4021 gttgtgcgcg acgtccagca ccagcgccgg ctgccccggc acgatctgga agcggcccgg
     4081 caggtcgatc gacgccaggc cgacgcgcac cgcctgcgcg gtgatcggca gccggtcgcg
     4141 cagcgtctcg aacgcggcca gcgcgcccga cgcgttgagc agctggttgg caccgcgcag
     4201 cgacggatag ccgagcgcgt tccagcgccg gccgcgcccg ctccacgacc actgctggcg
     4261 gtcgccggcg tagttgaagt cgcggccgat cagccacagc tcggcgccga tctgcgtggc
     4321 gtagtcgatg atgctctgcg gcggcaccgg gtcgccgacg accagcggcc ggcccttgcg
     4381 cgcgatgtgc gccttctcgc ggccgatcga ttcgcggtcg gggccgaggt attccacgtg
     4441 gtcgatgtcg atgctggtga tgaccgagac gtccggatcg accgcgttga ccgcgtccag
     4501 ccggccgccg aggcccacct cgaggatgac gaggtcgacc ggcgccgccg ccagcgcgcg
     4561 catgatcacc agcgtcgtga actcgaagta cgtgagcgcg atgtcgccgc gcgcggcctc
     4621 cacggcctgg aagtgcggga tcaggtcgtc ggccgtcacc atcctgccgt tgacgcggca
     4681 gcgctcctcg aagtgcacca ggtgcggctt gatgtacgcg cccaccttgt agccggcggc
     4741 caggccgatc gcctcgagca tcgcgcaggt cgagcccttg ccgttggtgc ccgccacggt
     4801 gatcaccggc acggcgaact tcagtccgag ccgctcgatc agcgtcttcg tgcgggcgag
     4861 cgtcatgtcg atcgtcttcg ggtgaaggcg ttcgcagtgg gcgagccatt cggcgagggt
     4921 ggtgggctgg gtcatggaaa gagcgggttc cgcgaccgcg cgcgaaagaa aggggtctgg
     4981 ctcccgccag aatgccgcca cccggaggct ggcgggcggt gccggcgggt gcctgacccc
     5041 gatggcggac ctggcatgcg agcggggtca ggcacccgcc cgcaacttgg tcagccgcag
     5101 cgggcatcgt ctggcgggag ccagacccct cggtcaggcg acggcgtcgg cgctttgccg
     5161 ctgcagctgc gccagcaggc gcgcgatcgt cggacgcagc ttgcggcggt cgacgatcat
     5221 gtcgatggcg cccttctgca gcaggaactc cgagcgctgg aagccctcgg gcagcttctc
     5281 gcgcacggtg ttctcgatca cgcgcgggcc ggcgaagccg atcagcgcct tgggctcgcc
     5341 gatgacgacg tcgccgatga acgcgaagct ggccgagacg ccgcccatgg tcgggtcggt
     5401 gagcacgctg atgtagggca gcttcgcctt ggccagcagc gtgagcgcgc cgttggtctt
     5461 ggccatctgc agcaggctga acaggccctc ctgcatccgc gccccgccgg tggcggtgaa
     5521 gcagatgaag ggcacgcgct gctcgaccgc cgtctgcacg ccacgcacga agcgctcgcc
     5581 gacgacggag cccatcgagc cgcccatgaa gtcgaactcg aagcaggcga cgaccgccgg
     5641 cacgctcatg atggagccgc ccatcacgac gagcgcgtcg gtctcgccgg tggcctccag
     5701 cgcgccctgc aggcgctcgg ggtacttgcg gctgtccttg aacttcaacg ggtcgaccgg
     5761 gatgacttcc tggccgatct cgaagcggcc ctccggatcg aggaagccgt cgagccgcgc
     5821 ccgggcgccg atgcgcaggt ggtgatcgca cttggggcag acgttcaggt tctgctcgag
     5881 gtcggtcttg tagagcaccg tttcgcagga cgggcacttg atccacagcc cttcggggat
     5941 cgtgcgccgc tcgctcggat ccgtttgctg gatcttcggg ggcaacagct tctcgagcca
     6001 actcatcaat cactccttga acgcaccggg aacccggcgc ttccttcgtg ctccggcgga
     6061 ccggagcggg gcaggcgatt ttacgccttc cggatctcgt ccacggccga gcggatcccg
     6121 gcgatgaacc ggcgggcccc gtcggccacc tggtcgcgcg gcagcgcctc caggcattgc
     6181 accagcgccg agccgatgac cacggcgtcc gacaccaggg ccaccgcctt ggccgaggcc
     6241 gcgtcccgga tgccgaaacc gaccccgacc ggcaccttca cgtgttcctt gatccgaggc
     6301 acggcgcgtg ccaccgcgtc ggtgtccagg tgcccggcgc cggtcacgcc cttgagcgag
     6361 acgtagtaca cgtagccggc ggccacctcg gccacctgct tcatgcgctt ctcggtcgag
     6421 gtgggcgcca gcaggaagat cggcgcgagc ccggccgcct tcagcgcgcg cgagaactcc
     6481 agcgactcct cgggcggata gtccaccacc agcacgccgt cgacgccagc ctccgcggcc
     6541 gcgcgaacga atgcgccctt gccgtgcgcc aggtcgtagg cctcgaccgg attggcgtag
     6601 cccatcagca cgaccggcgt cgcggcgtcg cgcacgcgga acgcgcgcac ggcctccagc
     6661 acctgcggcg tgccgatgcc gcgcgccagc gcccgctcgg acgccttctg gatgaccggg
     6721 ccgtcggcca tcgggtcgga gaacggcacg cccagctcga tgacgtccgc gccgccctcg
     6781 gccagcgcca gcatgacgtc gaccgtggcg tcggcgtacg gatcgccgca gcagatgtag
     6841 ggcacgaccg cgctgcggcc gtcggccttc agcttggaaa agacggtatc gatgcggcca
     6901 gtcattggga agcctccgtg ctgcgcactg cggcgtgccc gccttcgggc ggccgggcgt
     6961 cgggcggcgc acctccgatc gccttgccgc ccttgacgga ctgccccgcc atcgaaggcc
     7021 ggtcgtagaa cgccgtgccc gacaggtcgg cgacggtgcc gatgtccttg tctccgcggc
     7081 ccgacaggtt cacgagcacg tgctggtcgg gccgcatcgt cgcggccagc ttcatgccgt
     7141 atgcgaacgc gtggctggat tccagcgcgg ggatgatgcc ctcggtgcgg cacaggtggt
     7201 ggaacgccgc cagcgcctcg tcgtcggtga tgccgacgta ctcggcccgt ccgatgtcct
     7261 tgaggtacgc gtgctccggg ccgacgccgg gatagtccag ccccgccgag atcgagtgcg
     7321 tctcggtgat ctggccattg gcgtcctgca gcaggtaggt gcggttgccg tgcagcacgc
     7381 cgggcgagcc gcgcgtcagc gacgccgaat gcttgccgga gtccaggccc aggcccgcgg
     7441 cctcgacgcc gatcaggcgc gtgccctcgt gcgggatgta cgggtagaag atgcccatcg
     7501 cgttgctgcc gccgcccacg caggcgacga cggcgtcggg ctggcggccg gccatctcgg
     7561 gcatctgctc caggcattcc tcgccgatga cgctctggaa ctcgcgcacc atcgtcgggt
     7621 acgggtgcgg cccggcgacc gtgccgatca cgtagaacgt gttctcgacg ttggtcaccc
     7681 agtcgcgcag cgcctcgttg agcgcgtcct tgagcgtgcg gctgcccgac tccaccggca
     7741 ccaccttggc gcccagcagg tgcatgcggt agacgttggg gctttggcgc ttcacgtcct
     7801 cggcgcccat gtagacgatg cactcgaggc cgtagcgcgc gcagatcgtg gcggtggcca
     7861 cgccgtgctg gccggcgccg gtctccgcga tgatgcgcgg cttgcccatg cggcgcgcga
     7921 gcagcgcctg gccgatggtg ttgttgatct tgtgcgcgcc ggtgtggttc aggtcctcgc
     7981 gcttgagcca gatctgcgcg ccgcctacct cgcggctggt gcgcgccgcg tggtagatcg
     8041 ggctcgggcg gccgacgaag tgcttgagct cgtggcggaa ctcgcgctgg aagtcggggt
     8101 ccttgcggta gtgctcatag gccgcgttca gttcctcgat cgcgtgcgtg agggtctcgg
     8161 cgacgaagct gccgccgtac gggccgaagt ggcccgacgg gtcgggttgg gaatactcga
     8221 acatgatgga ctgctttcag gtgtcggcgc ccttggctcg ggcgccgtcg aagcaggagg
     8281 cgttggctcg ctgcttcagg tcatggcgtt ggcgacgcgg gcatcggcct cgcgtaccgc
     8341 ctcgcagaac cggcggatca ggacggcatc cttgatgccc ttggcctgtt ccacgccgga
     8401 actcacgtca acggcccagg gccggacgcg catcactcca tcgatcacgt ttgcaggact
     8461 caacccacca gacaaaacga ctggaagggg gacgcctgac ggaatttgag accaatcgaa
     8521 agcctttccg ccgccgccat agccctcgac gaacgcatcg agcagcaggc cggccgcgct
     8581 ggcgtagcca gaagcgcagt ctagcaaatc gacgccgggc gccatgcgca cggcgcgcag
     8641 gtagggccgg ccgggctgct cgcactgctc gcgcccctcg tcgccgtgga actgcagcag
     8701 cgcggtgggc accgcggcga cggcctcgtc gatcaggtcc gggctggcgt tgacgaacag
     8761 gcacacgggc gtgacgaacg gcggcaggca cttggccagc gcggccagcc gcgcgaccgg
     8821 cacgtgccgc gggctgcgct cgtacagcac gaagccgatc gcgtcggcac cggcagcgac
     8881 ggcggcgtcg atgtcctgct cgcgggtcag gccgcagatc ttgatgcggg tgcgcacgtg
     8941 gctcaattga cgatcctccg tgctgcgcac tgcggtgttc gcgcttcgga acggccgtgc
     9001 gcgctcatgg tcggggcgag gtgatgagcg gccgcgggtc gtcggcgtgc agcagttcga
     9061 ccggcacgcg cggcagccag tcgtgcgcgg cggtgcggcg cgggatgccc aggtgctggt
     9121 cgtactgcgg cccgacgaag tacaggccgg ccggcgggaa ggtgggcggc gcgacctcgc
     9181 ggttgcgctc ggccagcagc tcgctcatgt aggccgccgg ctggttgccg ctgccgatgg
     9241 tgatcagcgc gcccatgatg ttgcgcacca tgtggtgcag gaacgcgctg gcctcgaagt
     9301 cgaagcgcca gtagtcgccg atgcggttga tgtcgatgcg gcgcatctcc ttgaccggcg
     9361 acagcgcctg gcactgcgac gagcggaagc agctgaagtc gtgttcgccg atcagcgcct
     9421 gcgcggcctc gcgcatggtg tccaggtcga gcggccggaa cacccagccg cacaggccgg
     9481 cctcgatggc cgggcgcacg gccgactcgc gcacgaggaa cgcgtagcgc cggccgcgcg
     9541 cgccgttgcg tgcatggaag ccgggctcga cgttctgcgc ccattggacg gcgatgtcct
     9601 cgggcaggta gcggttggtg ccgcgaaccc acgagaagat ctcgcgcgtg acgggcgcgt
     9661 cgaagtgcac cacctggttg agcgcatgca cgcccgcgtc ggtgcgcccg gcgcacacgg
     9721 tggggatgat ctcgccgcgc ggcagtccga cgaactcggc gagcgcgcgc tcgagcttgt
     9781 cctgcacggt gttgccgtcg ggctgcgact gccagccgtg gtagttcagt ccgcgatacg
     9841 cgacgcccaa agcgacacgc ccgggcggaa ccgccgcgtt ggcggcgtcc tgctcgggcg
     9901 cggaggaggt ctcggattcg ggaccgccgg gcgccaggcc cggcgtggcc gtgggatctt
     9961 gaggggtggt cggattcacc ccatgaaggt taacccaagg cgtccagcat cgtctgtgcg
    10021 cgggacttca gagggccatc ggccttggag acgacctcct ccagcagctc gcgcgcgcct
    10081 tcgtggtcgc cgatgcggcg gaactcgtcg gccagttcga tcttgcgggc catcggctcg
    10141 gtctcgccgc cgccgcccag gtcgagctcg ggcagcaccg actcttccgc gacggccgcg
    10201 ccgccatggc ccggcttgtc caggtccagc gacaggtcgc cgaagtcgaa gtcgatcgac
    10261 ggcgcgccgg ccgcggcggg agccggggcg tgcgcatgca cgggcgccgg cgcgggcgcg
    10321 ggcggaatgg caggcacttc gacgtggccc ggctcgagat cgaagtcgct gacctcgaac
    10381 ggcatgtcgg ccgagacgct gtgcggctgg cgcgtggtgt cgccgtcgtg ctcctcggta
    10441 ggcatcggcc gcgtcggctg ctgcgtgtcg cccggctgga cggtgggcgg ctcgtcgctg
    10501 tcgaagtcga tcggcaggtc ggtgtcgtgc gcgccgccct tggccggcgg acgcatgagc
    10561 gtgacctcgt ccgagtcctc gcccagcggg ttcaggggca tcgggtcggt gtcgacgatg
    10621 gcgtactcgc cgccctcggt gtacagcgga ttggtcgggt cgatctggcg gccgagttcc
    10681 tgcgccttgg cccactcctc gccctgcccg ttcgtcaggc cctgcagctg gcgcgcctgc
    10741 gcctcgaagg tcttgaggtc ggcacgcttg gcatagacct cgagcagctt ggtgcggatg
    10801 gccaggcgat ccgggtcgga gcgctgggct tccttcagga tctcctcggc ctgcaggtcg
    10861 cggccgtagg cgaggtagac gtcggcctcg gcgaccgggt cgacgtcacc gatggcgtcg
    10921 agctggctca gcgagtagtt cagcgacgac gacgccgagt tggccgcgcc gtcgcgcgtg
    10981 tccacgcgct ggccgccgct ggcgccgaag aacgagtcgg gctggatctt gctctcgatg
    11041 aacgacgtct cgcccgagaa cttgttgcgg tcgccgccct ggcgcttgcg ccagaacagg
    11101 aagccgccca gcagcaccgc caggccggcg atgcccggca cgatgtacgg cgggtccatc
    11161 agcgaggaca ggaagctgtc gtccgccgcg ggggccggcg cgaccggcgc gggcgccggg
    11221 gccgaggcgc gcacgggcgc gggcggcgcc accaccggcg cgggcgccgg cgtggaggcg
    11281 accatggccg gggccggcgc ggaggcgacg ac
//
LOCUS       DATCRO010000345         7361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_172447, whole genome shotgun sequence.
ACCESSION   DATCRO010000345 DATCRO010000000
VERSION     DATCRO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172447"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /gene="dnaE"
                     /locus_tag="VIN75_09455"
     CDS             <1..2083
                     /gene="dnaE"
                     /locus_tag="VIN75_09455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8974432.1"
                     /translation="IYARLEAPELDQREKAEEEVAELLSLAARVEGIVRNIGMHAGGV
                     LIAPGKITDFCPLYQQPGSDSAVSQYDKDDVEAIGLVKFDFLGLATLTILELAKDFIV
                     ARRPGRENFSYETLPLDDPKVYRLFSDGLTEAVFQFESRGMQGMLRDAKPSRLEDLIA
                     LNALYRPGPMDLIPTFVARKHGKEQVEYPHPLVEPVLSETYGIMVYQEQVMQTAQVLG
                     GYSLGGADMLRRAMGKKKAEEMAKHREIFREGAAKNKIDTAQADEIFDLMEKFAGYGF
                     NKSHAAAYSLLAYHTGWLKVHCTAEFYAANMTIEMGDTDKLKVLLVDAKQFGITFTPP
                     DVNICVHRFEPAPGKAGDKLIHYGLGAVKGTGQGAVEAIVAAREGRTEQSDVARPFTS
                     IFDFAKRVDRSRVNKRVVDALVKAGAFDALHPDRACTLASVGLAFEWADAQAANAQQG
                     GLFDFGGGDEHGASTQEPALVPTEPWSIREKLTLEKTAIGFYLSGHMFDQYEPEVRKF
                     CKRRIADLIDSREPQVLCGIVSDLRIINGQRGRVAIFKVDDRSESIEAVANEELLDAN
                     RDCLRDDELLVVQGKVQPDRFTGGLRLNVTQIWDLAASRARFGRYLSVQADGNIGPLT
                     ELVKQWPAKRSLTEHGESVQGLGIRIKVRTQGAAAELDLGDQGRFWPSDDALARWKQI
                     MPAQPPQVVYD"
     gene            2108..2449
                     /locus_tag="VIN75_09460"
     CDS             2108..2449
                     /locus_tag="VIN75_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974433.1"
                     /translation="MRASWDGDPVSGFDEDFPAGFVGKVVLVGVTELALDGALASQEQ
                     LHGVIVAATAHGIDIALRGVHEGTTWRMPPWLDELVIARPGAYRLRSTGEVVEDPDFV
                     FSLTVHAAPTR"
     gene            2601..3641
                     /locus_tag="VIN75_09465"
     CDS             2601..3641
                     /locus_tag="VIN75_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974434.1"
                     /translation="MKRTLVAAAGFALFAASGLANAASWSVFVEPGVYGRVAIGGYAE
                     PPMVYAPQPVIAVDNGYGQVVEEVVDPAYATDQPPVYLWVPEYQRLHWAQYCREYGAI
                     GVPVYFVDDGWYRANVMRRNWTPEQRSWSQQQWLEQDRIARDRYERQRFEQQRFEQQQ
                     RWNAQREWYGQQQQGRWGHDHDRHGPPDGQNQIRWGSAQGYLPPQGNPGARPQPQPQP
                     GGGQWQGNGQWQQPGTGGHAGNGGHAGGGYAGGSGGSTGGSGAYPGGYAGNGGYTGGH
                     GTYGGNAGQQPPSASQPRGDGRGWQGGGQNAGERRGDDRGQDRGQRDERRAPPERHGG
                     FEHGRSGGGRER"
     gene            3796..4281
                     /locus_tag="VIN75_09470"
     CDS             3796..4281
                     /locus_tag="VIN75_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974435.1"
                     /translation="MDATPASKPAGNASMPGSITSLPATYGDVLAPLDVTSFPPSLAT
                     LHRDDALFRDWVQALDRTVRAEKVSSWTTWTAAERRAYDAGDTVAFSRLRGYTEAEIA
                     DYLRYLELTQALDAAHPDDPDFSFCSMHDVLQTMRTPAFDALDAHLQALSDAAPPGTH
                     P"
     gene            4444..5670
                     /locus_tag="VIN75_09475"
     CDS             4444..5670
                     /locus_tag="VIN75_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8974436.1"
                     /translation="MPAAPTTGLILTGGGARAAYQVGVLRAVHRIRREAGAPPGNPYP
                     VLAGTSAGALNAAALATDADDADAGIAKLVRVWENMHVEQVYRTDSFGVIRTGAKWVT
                     MMSMGWAIARMRKLRPRSLLDNQPLHGLLAEMIDLDRIDRLIADGHLRALAVSASSYT
                     SGSHVTFYQAEARHEPWVRSNRIAVQGPLTMAHLLASAAIPFMFPAVTLPLQGAYEWF
                     GDGSMRQTAPISPAIHLGADRILVIGAGRMREPPGPRTMVPSPYPSIAQIGGHALSSI
                     FLDALAVDAERLQRINATLSLIPEEVRTHATLKPVQLLLISPSERIDDIAARHLDALP
                     GSIRSLLRGIGVSNAGPGSNGAALASYLLFEDAFTRDLMALGEKDTDARRAEVLQFFD
                     LPADATAPAVPATAAA"
     gene            5702..6190
                     /locus_tag="VIN75_09480"
     CDS             5702..6190
                     /locus_tag="VIN75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974437.1"
                     /translation="MTTAQPGTTPRAAVTVRRARAADAGAVAALYAQLVANPALHVLP
                     ARLDELAAHADAALLVASVDGTLVGTVFVAWCPDAMFGRRPFAVVENIVVDTGSRGRG
                     VGAALLAEVERLSRARDCSKIMLLSASERVAAHRFFVRGGYAGDRKRGFVKYRSAFAS
                     AD"
     gene            complement(6455..7051)
                     /locus_tag="VIN75_09485"
     CDS             complement(6455..7051)
                     /locus_tag="VIN75_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8974438.1"
                     /translation="MIKRILAALLAFFAFSVFAADANTATQAELDAVKGIGPATATKI
                     IEERKNGAFKDWPDLIARVKGIGDKNAAKMSEGGLTVNGAAMSGAPAAPAKPAAPAAK
                     PATPAPAPAPAPAAKPATPAPAPAPAPATTKPAAPAAPAAPASSAKELKKAAAAQAKA
                     DKASAAAQAKADKAAKAAQKKADKAAAAASAASAAKKK"
BASE COUNT         1087 a         2622 c         2624 g         1028 t
ORIGIN      
        1 gatctacgcc cgcctcgagg cgcccgagct cgaccagcgc gagaaggccg aagaggaagt
       61 cgccgagctg ctgtcgctgg ccgcgcgcgt ggaaggcatc gtccgcaaca tcggcatgca
      121 cgccggcggc gtgctgatag cgcccggcaa gatcaccgac ttctgcccgc tgtaccagca
      181 gccgggcagc gactcggccg tcagccagta cgacaaggac gacgtggagg ccatcggcct
      241 ggtgaagttc gacttcctgg gcctggccac gctgaccatc ctggaattgg cgaaggactt
      301 catcgtcgcg cgccgaccgg gccgcgagaa cttctcgtac gagacgctgc cgctggacga
      361 cccgaaggtc taccgcctgt tctccgacgg cctgacggaa gccgtgttcc agttcgaatc
      421 gcgcggcatg cagggcatgc tccgcgacgc caagcccagc cgcctggaag acctgatcgc
      481 gctgaacgcg ctgtaccgcc ccggcccgat ggacctgatc ccgaccttcg tcgcgcgcaa
      541 gcacggcaag gagcaggtgg agtacccgca cccgctggtc gagcccgtgc tgtccgagac
      601 ctacggcatc atggtctacc aggagcaggt gatgcagacc gcccaggtgc tgggcggcta
      661 ctcgctcggc ggcgcggaca tgctgcgccg cgcgatgggc aagaagaagg ccgaggagat
      721 ggccaagcac cgcgagatct tccgcgaggg tgcggccaag aacaagatcg acacggccca
      781 ggccgacgag atcttcgacc tgatggagaa gttcgcgggc tacggcttca acaagtcgca
      841 cgccgccgcg tactcgctgc tcgcctacca cacgggttgg ctgaaggtcc actgcaccgc
      901 cgagttctac gcggcgaaca tgaccatcga aatgggcgac accgacaagc tcaaggtgtt
      961 gctggtcgac gccaagcagt tcggcatcac gttcacgccg cccgacgtca acatctgcgt
     1021 gcaccgcttc gagcccgcgc cgggcaaggc gggcgacaag ctgatccact acggcctggg
     1081 cgccgtcaag ggcacgggcc agggggcggt ggaggccatc gtggccgcac gcgaggggcg
     1141 cacggaacag agcgacgtgg cgcgcccgtt caccagcatc ttcgacttcg ccaagcgggt
     1201 cgaccgctcg cgcgtcaaca agcgcgtggt cgatgcgctc gtcaaggccg gcgccttcga
     1261 tgcgctgcat cccgaccgcg cctgcacgct ggccagcgtg ggcctggcct tcgagtgggc
     1321 cgatgcgcag gcggccaacg cgcagcaggg cggcctgttc gacttcggcg gcggcgacga
     1381 gcacggcgcg tccacgcagg agccggcgct ggtgcccacc gagccgtgga gcatccgcga
     1441 gaagctcacg ctggagaaga cggccatcgg cttctacctg tctggccaca tgttcgacca
     1501 gtacgagccc gaggtgcgca agttctgcaa gcgccgcatc gccgacctga tcgactcgcg
     1561 cgagccgcag gtgctgtgcg gcatcgtgag cgacctgcgc atcatcaacg gccagcgcgg
     1621 gcgcgtggcc atcttcaagg tggacgaccg atccgaatcg atcgaggccg tggccaacga
     1681 ggaactgctc gatgccaacc gcgactgcct gcgcgacgac gaactgctgg tggtgcaggg
     1741 caaggtgcag cccgaccgct tcaccggcgg cctgcggctg aacgtcacgc agatctggga
     1801 cctggccgcg tcgcgcgccc gcttcggccg ctacctctcg gtgcaggccg acggcaacat
     1861 cgggccgctc accgaactcg tcaagcaatg gccggccaag cgctcgttga ccgagcacgg
     1921 cgagtcggtg cagggcctgg gcatccgcat caaggtgcgc acgcagggcg ccgccgccga
     1981 gctcgacctc ggcgaccagg gccgcttctg gcccagcgac gatgcgctgg cgcgttggaa
     2041 gcagatcatg ccggcgcagc cgccgcaggt ggtctacgac tgaggtgccg caggcgggaa
     2101 gcagcgcatg agagcgtcgt gggacggcga tccggtgtcg gggttcgacg aggacttccc
     2161 ggccgggttc gtcggcaagg tcgtgctcgt cggcgtgacc gaactggcgc ttgacggcgc
     2221 cttggcgagc caggaacaac tgcacggcgt gatcgtggcg gcgacggccc atggcatcga
     2281 catcgcgctg cgcggtgtcc atgaggggac gacgtggcgc atgccgccct ggctcgacga
     2341 actggtgatc gccaggcccg gcgcgtaccg gctgcgctcg acgggcgagg tcgtcgagga
     2401 cccggacttc gtgttctcgc tgacggtgca cgccgcaccg acgcgctgat cgcccgggtt
     2461 tcccgctagg cggttcgccg ctggaagcat cgcgttgcgg catgtgtgcg caatgttgct
     2521 gggctttact caggcaactg acaactgtca ccaagctgac cacgttgaat gggtgcaccc
     2581 catttaacat ggagcccctc atgaaacgca ccctcgtcgc cgccgccggc ttcgccctgt
     2641 tcgccgcctc cgggctcgcg aatgcggcgt cgtggtcggt gttcgtcgag cccggcgtct
     2701 acggccgggt cgcgatcggc ggctacgccg agccgccgat ggtctatgcg ccgcaaccgg
     2761 tcatcgcggt cgacaacggc tacggccagg tcgtggagga agtggtcgac ccggcctatg
     2821 ccaccgacca gccgcccgtc tacctgtggg tgcccgaata ccagcgcctg cactgggcgc
     2881 agtactgccg cgaatacggc gccatcggcg tgccggtgta cttcgtcgac gacggctggt
     2941 accgcgccaa cgtgatgcgg cgcaactgga cgcccgagca gcgctcgtgg tcgcagcagc
     3001 agtggctcga gcaggatcgc atcgcgcgcg accgctacga acgccagcgc ttcgaacagc
     3061 agcgcttcga gcagcagcaa cgctggaacg cgcagcgcga gtggtacggc cagcagcagc
     3121 aaggccgctg gggccacgat cacgaccggc acggcccgcc ggacggccag aaccagatcc
     3181 gctggggctc ggcgcaaggc tacctgccgc cgcaaggcaa tccgggcgcg cgtccgcaac
     3241 cgcagccgca acccggcggc gggcagtggc agggcaacgg ccaatggcaa caacccggaa
     3301 ccgggggcca tgccggcaac ggcggccacg caggtggcgg ctatgctggc ggctcgggcg
     3361 gctccacggg cggcagcggc gcctaccctg gcggctacgc cggcaacggc ggctacacgg
     3421 gcggccacgg cacgtacggc ggcaatgccg gccagcaacc gccgtcggca tcgcaaccgc
     3481 gcggcgatgg ccgcggctgg caaggcggcg ggcagaacgc gggcgaacgc cgcggcgacg
     3541 accgcggaca ggatcggggc cagcgcgacg aacgccgcgc cccgcccgag cgccatggcg
     3601 gcttcgagca cgggcgctcc ggcggcgggc gcgagcgctg agccggcagg ccctgcgcgc
     3661 gagcccgaac cggcctgcga cgcaagcttc ccgggcgccg cgatcggtcg gagccggtac
     3721 gccgggcatc acgaccgccg atcgacgatg cgcccgcgcc agccgctggc cgacagcgcc
     3781 tcgtagactg cgaccatgga cgccacacct gcctccaagc cggccgggaa cgcttcgatg
     3841 ccaggctcga tcacgtcgct gccggccacc tacggcgacg tgctggcacc gctggacgtc
     3901 acgtcgtttc ccccgtcgct cgccaccctc catcgcgacg acgcgctgtt ccgagactgg
     3961 gtgcaggccc tggatcgcac ggtgcgcgcc gagaaggtgt cgtcgtggac gacctggacc
     4021 gccgcggagc gccgggccta cgacgccggc gacaccgtcg cgttctcccg cctgcgcggc
     4081 tacaccgagg ccgagatcgc cgactacctg cgctatctcg agctcaccca ggccctggat
     4141 gccgcccacc ccgacgaccc ggacttcagc ttctgcagca tgcacgacgt gctgcagacg
     4201 atgcgcacgc cggccttcga cgcgctcgat gcgcacctgc aggcgctgtc ggacgccgcc
     4261 ccgcccggaa cccatccctg aacgggcgag ctgcgcggac tcacgggtcg gacggccgca
     4321 ctcgcgcgag gcacgacccg tcgcgcgagc cgcaggccac ggtgatcgcc ccaccgtcgc
     4381 atccccgtca cccccgcgtc gccccggcta tccggcgtgc ccgacctcgc ggatactgcg
     4441 tccatgcccg ctgcacccac gaccggcttg atcctcacgg gcggtggcgc tcgcgccgcc
     4501 taccaggtgg gcgtgctgcg tgccgtgcac cgcattcggc gcgaggccgg ggcgccgccc
     4561 ggcaacccgt atcccgtgct ggccggcacg tcggccgggg cgctcaacgc ggcggcgctg
     4621 gccacggacg ccgacgacgc cgacgccggc atcgccaagc tcgtgcgcgt gtgggagaac
     4681 atgcacgtcg agcaggtcta ccgcaccgac tcgttcggcg tgatccgcac gggcgccaag
     4741 tgggtcacga tgatgtcgat gggctgggcc atcgcacgca tgcgcaagct gcgcccgcgc
     4801 tcgctgctgg acaaccagcc gctgcacggc ctgctggccg agatgatcga tctcgatcgc
     4861 atcgaccgcc tgatcgccga cggccacctc agggcgctgg ccgtgagcgc gtcgagctac
     4921 acctcgggca gccacgtcac gttctaccag gccgaggccc ggcatgagcc gtgggtccgc
     4981 tccaaccgaa tcgccgtgca ggggccgctg acgatggccc acctgctcgc gtcggccgcg
     5041 atcccgttca tgttcccggc cgtgacgctg ccgctgcagg gcgcgtacga gtggttcggc
     5101 gacggctcga tgcgccagac ggcgccgatc tcgccggcca tccacctggg cgcggaccgc
     5161 atcctcgtga tcggcgcggg ccgaatgcgc gagccgcccg ggccgcgcac gatggtgccg
     5221 tcgccgtatc cgtcgatcgc gcagatcggc ggccacgcgt tgtccagcat cttcctggac
     5281 gcgctggcgg tggacgccga gcgcctgcag cgcatcaacg cgacgctctc gctgataccc
     5341 gaggaggtac gcacgcacgc gacgctcaag ccggtgcaac tgctgctgat ctcgccgtcc
     5401 gagcgcatcg acgacatcgc cgcgcgccac ctggacgcgc tgcccggctc gatccgctcg
     5461 ctgctgcgcg gcatcggcgt gtccaacgcc ggccccggca gcaacggtgc ggcgctggcc
     5521 agttacctgc tgttcgagga cgcgttcacg cgcgacctga tggcgctcgg cgagaaggac
     5581 accgacgcgc gacgcgccga ggtgctgcag ttctttgacc tgcccgcgga cgcgacggcg
     5641 cccgccgtgc cggccacggc ggcggcatga cggcgcggac gtgcagcgcg gtaccggccg
     5701 catgacgacg gcgcagcccg gaacgactcc gcgcgccgcg gtcacggtcc ggcgcgcccg
     5761 cgcggccgac gccggcgccg tcgccgcgct ctatgcgcaa ctggtggcca accccgcgct
     5821 gcatgtactg cctgcacgcc tggacgagct ggccgcgcac gccgacgcgg cgctgctggt
     5881 cgcgagcgtc gacggcacgc tcgtgggcac cgtcttcgtc gcctggtgtc ccgacgcgat
     5941 gttcggccgc cggccgttcg ccgtggtcga gaacatcgtc gtcgacacgg gctcccgcgg
     6001 ccgcggcgtg ggcgccgcgc tgctggccga ggtcgagcgc ctcagccgcg cgcgcgactg
     6061 ctccaagatc atgctgctga gcgccagcga acgcgtcgcg gcgcaccgct tcttcgtgcg
     6121 cggcggctac gccggcgacc gcaagcgcgg cttcgtgaag taccggtcgg cgttcgcgtc
     6181 ggcggactga gtcgcgccag ggggccgccc gcgagtctgc acagccgtca tcctgcgcat
     6241 agccgtcatc ctgcgcgcag tcgcaggatc cacccctgcc aggcggcctc gtgcgatgcg
     6301 tggatcgtgc gactgcgcgc aggatgacgg cttggcttgc gagtgccgac ctggcggccc
     6361 gcacgaccac cccgtgcggg accaccacga aaaacgcccc ggcaaacggg gcgtttcgag
     6421 agcgtggcgt cgcgggccct ggcccgccgg ccaattactt cttcttcgca gccgaagcag
     6481 ccgacgccgc ggcggcggcc ttgtcggcct tcttctgcgc ggccttggcg gccttgtcgg
     6541 ccttggcttg cgccgcggcg gacgccttgt cggccttggc ctgggcggcc gcggccttct
     6601 tcagttcctt ggcggagctg gccggcgcgg cgggagcggc gggggcagcc ggcttggtcg
     6661 tggccggagc gggcgccggc gcaggggcgg gcgtcgcggg cttggcggcg ggcgccgggg
     6721 cgggcgcggg agccggggtg gcgggcttgg cggccggggc ggcgggcttg gccggggccg
     6781 cgggcgcgcc ggacatcgcg gcgccgttga cggtcaggcc gccctcggac atcttggcgg
     6841 cgttcttgtc gccgatgccc ttgacgcggg cgatcaggtc cggccagtcc ttgaaggcgc
     6901 cgttcttgcg ctcctcgatg atcttggtgg cggtggccgg gccgatgccc ttgaccgcgt
     6961 cgagctccgc ctgggtggcg gtgttcgcgt cggcggcgaa caccgagaag gcgaagaagg
     7021 cgaggagggc ggcgagaatg cgtttgatca tggctgcgtc cttgggttga cagcgttgtg
     7081 cgcgcgggtg ggggcgcgac gacccgttgc ggcgcccggc aaagggatgc cgcatgccct
     7141 ctcaacgcgg gcggtgccgg ccggttgacg tttcttcacg aaaccgccgc cgggcgtgat
     7201 gcccgactca ggcctgttgc aggtccgcca ggatggtcgt gaccagggtc tggcaccagt
     7261 gcgcgttggt gaagcgcttc gcgtcctcgc tggcgaggaa ttcgcggatg ccggcctggt
     7321 acatcgcgaa gtggccggcg tcgatgcgat cgatgtaggc c
//
LOCUS       DATCRO010000346         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1005132, whole genome shotgun sequence.
ACCESSION   DATCRO010000346 DATCRO010000000
VERSION     DATCRO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005132"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VIN75_09490"
     CDS             <1..335
                     /locus_tag="VIN75_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8974439.1"
                     /translation="PEVLARRQLDLARLEAAVDAALHEDWPTARLAALCCLSAQRFHA
                     RLLELTGRSPQAWLRALRLDEAQRLLRRGLTLEAAAARVGYATASALAHALRRDREIG
                     ARALRDDD"
     gene            389..1288
                     /locus_tag="VIN75_09495"
     CDS             389..1288
                     /locus_tag="VIN75_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8974440.1"
                     /translation="MKTASWPGIVLALAAAVLWGTTGTSQHLAGAHLSSCWIGALRLV
                     IAAAFFAGLVALTGRGSPARALPAGLWKRQLLAGAAIAAYNLAFFAGVRLAGVAVGTT
                     VAIGSGPLFAGALQALVTRRAPVPLWWLGTALAVAGGAAIALGGGGAASASLGGLLPC
                     LAAGLSYAVYTLTAKSLSGLASPARASLWVFGTAAVIALPAAWALVPSGPADLAAAGT
                     RTWLVVAWLGVVATGVSYLLFSTALRFISGATGVALALGEPLTAFTLAVLLLGEPLRP
                     GGLVGIALILAGLALVITAERRG"
     gene            1304..2179
                     /locus_tag="VIN75_09500"
     CDS             1304..2179
                     /locus_tag="VIN75_09500"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8974441.1"
                     /translation="MTRQSSLLIIGCGDVGLRVVRALGGRLRVFALTSSPARCDALRA
                     AGVRPVVGNLDDPASLGRLAGLADAVLYLAPPPTTGRCDPRLAALLAAISRRSLPARV
                     VYASTTGVYGDCRGELVVESRPTNASTDRAVRRVDAESRLRAWARATGVRASVLRIPG
                     IYASDREGGRLQDRVRRGTPVLAAADDGYTNHINADDLARACIASLWRGRPQRVVNVC
                     DDSDMKTGDYYDLVADLVGLPRPERVTRAQAGERFSAAQWSFLNESRRLSNARMKREL
                     RLRLQRPTIREGLAD"
     gene            complement(2189..2845)
                     /locus_tag="VIN75_09505"
     CDS             complement(2189..2845)
                     /locus_tag="VIN75_09505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8974442.1"
                     /translation="MQAPPPITRVLLIACTALLFASQIPVLGMLMSQWLALHPALSGF
                     WPWQLLTFGFVHVQVLNWLFNMMMLYYFGSELEGIWGERRYIQFLLASALAAAAVYLL
                     LTLLPPLLGWSLPAAPLYDSTSIAFGILIAVAILFPHRPIRLFFVLDVTQRVAAWIFL
                     GISVFVMLGDFGYGSAAWARDLAQFGGALGGYLMLLYWRWRPPSFKRKKSPPHIRRVH
                     "
     gene            complement(2957..3313)
                     /locus_tag="VIN75_09510"
     CDS             complement(2957..3313)
                     /locus_tag="VIN75_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8974443.1"
                     /translation="MSKRTVLALGVAALLAATAAHASGKPVIGISFSIETDGFFSTTL
                     KKVTVTAVKPGLPAEKAGVQAGDEIVAANDIPIAGTPGGKLKDVLESARPGDHLKLRV
                     HRGSAEQVCDVVVGER"
     gene            complement(3458..3542)
                     /locus_tag="VIN75_09515"
     tRNA            complement(3458..3542)
                     /locus_tag="VIN75_09515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3506..3508),aa:Leu,seq:caa)
     gene            3671..4576
                     /gene="cysM"
                     /locus_tag="VIN75_09520"
     CDS             3671..4576
                     /gene="cysM"
                     /locus_tag="VIN75_09520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY8974444.1"
                     /translation="MPTYMTLEEAIGHTPLVRLQRVIGAEIAARGNVVLGKLEGNNPA
                     GSVKDRPAIAMIRGAEERGQIKPGDTLIEATSGNTGIALAMAAAIKGYRMVLIMPEDL
                     SVERVQSMKAYGAELILTPKSGGMEYARDLATQMQVDGKGLVLDQFANPDNPRSHYET
                     TGPEIWKDTEGRITHFVSAMGTTGTITGVSRFLKEKNPAIRILGAQPSEGSRIPGIRK
                     WPEAYMPKIYDPSHIDETIQVTQPDAEEMARRLAREEGLFAGISAAGACWVALQVAQR
                     LENATIVFVVCDRGDRYLSTGVFPA"
     gene            4573..5145
                     /locus_tag="VIN75_09525"
     CDS             4573..5145
                     /locus_tag="VIN75_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8974445.1"
                     /translation="MTGTQWRFCPQCAHELSLVTQDEDGGPKERLRCASCGFTHWNNP
                     TPVLAAIVECADRDGRILLARNAAWAGRMFALITGFMEAGETPDEGIAREVLEETALT
                     VEKVDLVGVWDFQRMNQVIIAYHVVARGEIRLSPELAEYKLFAPGDIVCWPAGTGYAV
                     ARWLESRGITPRFMTAEERAALATPRPEAS"
     gene            5174..>5392
                     /locus_tag="VIN75_09530"
     CDS             5174..>5392
                     /locus_tag="VIN75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8974446.1"
                     /translation="MQAHKEIDTRGMNCPLPILKAKKALAEMQTGEILKVVATDPSST
                     RDFQAFARQTGNELLEQHQESTEYIHFLR"
BASE COUNT          784 a         1896 c         1959 g          753 t
ORIGIN      
        1 cgcccgaagt gctcgcgcgg cgccagctcg acctcgcccg gctcgaggcc gcggtcgacg
       61 ccgcgctgca cgaggactgg cccaccgcgc gcctggccgc gctgtgctgc ctgagcgcgc
      121 agcgcttcca cgcgcggctg ctggagctga cgggccgctc gccgcaggcc tggctgcgcg
      181 cgctgcggct ggacgaggcc cagcgcctgc tcaggcgcgg cctcacgctc gaggccgcgg
      241 cagcgcgggt cggctatgcc acggccagcg cgctggcgca tgcgctgcgg cgcgatcgcg
      301 agatcggcgc ccgggccctg cgcgacgacg actgagaagt cgagagtctc tcgacaattc
      361 cgcgccccgc ccgcgtcacc atggcggcat gaagactgca tcctggccgg gcatcgtcct
      421 ggcgctggcg gccgccgtgc tgtggggcac gaccggcacg tcccagcatc ttgccggcgc
      481 gcacctgtcg tcctgttgga tcggcgcgct gaggctcgtc atcgccgcgg cgttcttcgc
      541 ggggctggtg gcgctcaccg gacgcggctc gccggcacga gcgcttccgg ccggcctgtg
      601 gaagcgccag ctgctggccg gcgcggccat cgccgcctac aacctcgcat tcttcgccgg
      661 cgtgcggctg gcgggcgtgg ccgtgggcac cacggttgcc atcggcagcg ggccgctgtt
      721 cgccggcgcg ctgcaggcgc tcgtcacgcg gcgcgcgccg gtgccgctgt ggtggctcgg
      781 cacggcgctc gcggtcgcgg gcggcgcggc catcgcgctg ggcggagggg gcgcggcgag
      841 tgccagcctg ggcgggctgc tgccttgcct ggcggccggg ctctcgtatg ccgtctacac
      901 gctgacggcc aagtcgctgt cgggcctggc atcgccggcg cgcgcgagcc tgtgggtgtt
      961 cggcacggcc gcggtcatcg cgctgcccgc ggcgtgggcg ctcgtgccct cggggccggc
     1021 cgacctcgca gccgccggca cgcgcacctg gctcgtcgtc gcctggctgg gcgtggtggc
     1081 cacgggcgtg tcgtacctgc ttttcagcac cgctctgcgc ttcatctcgg gcgcgaccgg
     1141 cgtggcgctg gcgctgggcg agccgctgac agcgttcacg ctggccgtgc tgctgctggg
     1201 cgagccgctg cgacccggcg gcctcgtcgg catcgcgctg atcctggccg ggctggccct
     1261 ggtcatcacg gctgaacgcc ggggttgaga caatgcgccg ttcatgacgc gccaatcctc
     1321 cctcctgatc atcggctgcg gcgatgtcgg cctgcgcgtc gtgcgcgcgc tcggcggccg
     1381 cctgcgcgtg ttcgcgctga cctcgtcgcc cgcccgctgc gacgcgctgc gcgccgccgg
     1441 cgtgcggccg gtggtcggca atctcgacga cccggcgtcg ctcggacgcc tggctggtct
     1501 ggccgatgcg gtgctgtacc tcgcgccgcc gcccacgacc ggtcgctgcg atccgcgcct
     1561 ggccgcgctg ctggccgcga tcagccgccg ttcgctgccc gcacgcgtcg tgtacgcgag
     1621 cacgacgggc gtctacggcg actgccgggg cgaactcgtc gtcgagtcgc gcccgacgaa
     1681 cgcgagcacc gaccgcgccg tgcgccgcgt cgacgcggag tcgcgcctgc gcgcctgggc
     1741 gcgcgcgacg ggcgtgcgcg cgagcgtgct gcgcatcccc ggcatctacg cgagcgaccg
     1801 cgagggcggc cggctgcagg accgcgtgcg acgcggcacg ccggtgctgg cggcagccga
     1861 cgacggctac accaaccaca tcaacgccga cgacctcgcc cgcgcctgca tcgcgtcgct
     1921 gtggcgcggc cggccgcagc gcgtggtcaa cgtctgcgac gacagcgaca tgaagacggg
     1981 cgactattac gacctcgtcg ccgacctcgt cggcctgccg cgtcccgagc gcgtgacgcg
     2041 cgcgcaggcg ggcgagcgct tcagcgccgc gcagtggagt ttcctcaacg agtcgcgcag
     2101 gctctccaac gcgcggatga agcgcgagct gcggctgcgc ctgcagcgcc cgacgatccg
     2161 cgaaggcctc gcggactgaa gccgcgggtc agtgcacgcg ccggatgtgc ggcggcgact
     2221 tcttgcgctt gaacgacggc ggacgccagc gccagtacag cagcatcagg tagccgccga
     2281 gcgcaccgcc gaactgcgcg aggtcgcgcg cccaggcggc cgaaccatag ccgaagtcgc
     2341 ccagcatcac gaacacgctg atgcccagga agatccaggc cgcaacgcgc tgggtcacgt
     2401 cgaggacgaa gaacagccgg atgggccggt gcgggaacag gatggcgacg gcgatcagga
     2461 tgccgaacgc gatgctggtc gagtcgtaca gcggcgccgc cggcagcgac cagcccaaca
     2521 gcggcggcag cagcgtcagc agcaagtaga cggcggcggc cgccagcgca ctggccagca
     2581 ggaactggat gtagcggcgc tcgccccaga tgccctccag ctcggagccg aagtagtaca
     2641 gcatcatcat gttgaacagc cagttcagca cctgcacgtg cacgaagccg aacgtcagca
     2701 actgccacgg ccagaagccg gacagcgccg ggtgcagcgc cagccattgg ctcatcagca
     2761 ttcccagcac gggaatctgc gaggcgaaca gcagcgcggt gcaggcgatc aacaggacgc
     2821 gggtgatggg cggcggagct tgcatggcgg gtcggcgacg atgaccgccc agtgtgccat
     2881 ggggccgcgg cgggcgggcc cggcggcgcg cgccgcaccc gtggcgaatt cacgccgcgg
     2941 ccgggcgagc gggctttcag cgttcgccga ccaccacgtc gcagacctgc tccgcgctgc
     3001 cgcggtgcac gcgcagcttc aggtggtcgc ccggcctggc gctctccagc acgtccttga
     3061 gcttgccgcc cggcgtgccg gcgatgggga tgtcgttggc ggcgacgatc tcgtcgcccg
     3121 cctgcacccc ggccttctcc gcgggcagcc ccggcttgac ggccgtcacg gtcaccttct
     3181 tgagcgtggt gctgaagaag ccgtcggtct cgatggagaa gctgatgccg atcacgggct
     3241 tgccggaggc gtgggcggcg gtggcggcga ggagtgcggc gacgccgagg gcgaggactg
     3301 tgcgcttgga catgatgcga tcgttcacga ggatggcgcg agtctgcccg acgttcgagt
     3361 ggcgatccat gcgcgtgcga cggtcggtgt cattcggagc ctgggccgca gccgggaatc
     3421 ggcgcggcgt tccatgcgac gcgaaggtcg gtcgccttgg tgcctcgggt cggactcgaa
     3481 ccgacacccc tttcgagaac ggattttgag tccgtcgcgg ctaccgatta cgccaccgag
     3541 gcaggggatg ctgcgggcgt atgcacgtgc gcagggggcg gattatcaca tgacgaaggc
     3601 gaggtttggg acgcactgcg acaatgcggg ctccttgtcc agctgatcgt tccaacggat
     3661 tgccccaccc atgccgacgt acatgaccct cgaggaggcc atcggccata cgccgctggt
     3721 gcggttgcaa cgcgtcatcg gcgccgagat cgccgcgcgc ggcaacgtcg tgctcggcaa
     3781 gctggagggc aacaacccgg ccgggtcggt caaggaccgg cccgcgatcg cgatgatccg
     3841 cggcgccgag gagcgcggcc agatcaagcc gggcgacacg ctgatcgagg ccacctcggg
     3901 caacaccggc atcgcgctgg cgatggccgc ggcgatcaag ggctatcgca tggtgctgat
     3961 catgccggag gacctgtcgg tcgagcgcgt gcagtcgatg aaggcctacg gcgccgagct
     4021 gatcctcacc ccgaagtcgg gcggcatgga atacgcgcgc gacctcgcca cgcagatgca
     4081 ggtggacggc aagggcctgg tgctcgacca gttcgccaac ccggacaacc cacgctcgca
     4141 ctacgagacc accggccccg agatctggaa ggacaccgag ggccgcatca cgcacttcgt
     4201 gagcgcgatg ggcacgacgg gcacgatcac cggggtgtcg cgcttcctca aggagaagaa
     4261 cccggcgatc cgcatcctcg gcgcgcagcc gtcggaaggc tcgcgcatcc cgggcatccg
     4321 caagtggccc gaggcctaca tgcccaagat ctacgacccg tcgcacatcg acgagacgat
     4381 ccaggtgacc cagccggatg ccgaggagat ggcgcgccgc ctcgcgcgcg aggaaggcct
     4441 gttcgcgggc atctcggccg ccggtgcgtg ctgggtcgcg ctgcaggtgg cgcagcggct
     4501 ggagaacgcg acgatcgtct tcgtcgtgtg cgaccgcggc gaccgctacc tgtcgaccgg
     4561 ggtgttcccg gcatgaccgg tacgcagtgg cgcttctgcc cgcagtgcgc gcacgagctg
     4621 tcgctggtca cgcaggacga ggacggcggc ccgaaggaac gcctgcgctg cgccagttgc
     4681 ggcttcacgc actggaacaa cccgacgccg gtgctcgccg ccatcgtcga atgcgccgac
     4741 cgcgacggca ggatcctgct ggcccgcaac gcggcctggg cgggccgcat gttcgcgctg
     4801 atcaccggct tcatggaggc cggcgagacg cccgatgagg gcatcgcgcg cgaagtgctc
     4861 gaggagaccg cgctgacggt cgagaaggtc gacctcgttg gcgtgtggga cttccagcgc
     4921 atgaaccagg tcatcatcgc gtaccacgtg gtggcgcgcg gcgagatccg gctgtcgccc
     4981 gaactggccg agtacaagct gttcgcgccc ggcgacatcg tctgctggcc ggcgggcacc
     5041 ggctacgcgg tggccagatg gctggagtcg cgcggcatca cgcccaggtt catgacggcc
     5101 gaggagcgcg ccgcgctcgc gacgccgcgg cccgaggcgt cataatcttg ggcatccaac
     5161 caggttccac tccatgcaag cccacaagga aatcgatacg cgcggcatga actgcccgct
     5221 gcccatcctg aaggccaaga aggcgctggc cgagatgcag acgggcgaga tcctgaaggt
     5281 cgtcgccacc gacccgagtt ccacgcgcga cttccaggcg ttcgcccgcc agacgggcaa
     5341 cgagctgctc gagcagcacc aggaatcgac ggagtacatc cacttcctgc gc
//
LOCUS       DATCRO010000347         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_757208, whole genome shotgun sequence.
ACCESSION   DATCRO010000347 DATCRO010000000
VERSION     DATCRO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757208"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VIN75_09535"
     CDS             <1..1233
                     /locus_tag="VIN75_09535"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEY8974447.1"
                     /translation="IELAGHQSYCNVSISVKQFGLIVGSVGHQLGDALLCQIAARLDH
                     VVRGFEGRLYRVDGVTFGALLQFGHDPKRLAGILEALTRAMGAPFEIGNQDLIVSIGV
                     GASEYPKDAGTADEVARLAELAKLQAHADPGNSFLVYDSRIGEVQRDKLRMESRMLKA
                     LEHGDFELFYQGQRGLPDGRIKGFEALLRWRDGDKMISPADFIPLAEETGLIVRIGTW
                     VLEEACRQARSWADTGAGRPIVAVNISPRQFNHPDFLATVRDTLARTGVDPRQIELEI
                     TEGSVMNDAEASIAQLHALRDLGLHLAIDDFGTGYASLSYLRRFPLDRLKIDRSFVKQ
                     LTMSEQDDTIVRTVIELAHTLGLSVTAEGVETIEQESVLQGWGCDVIQGFLRFRPAPA
                     DAATGLLEKDKQEALQAA"
     gene            complement(1441..2259)
                     /locus_tag="VIN75_09540"
     CDS             complement(1441..2259)
                     /locus_tag="VIN75_09540"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974448.1"
                     /translation="MKWLPEFPAYATPLDKAGWWALRVFCVAVLGFLLLPILVIVPLS
                     FSDSSFLVYPIPAFSTKWYQNLFGSAEWARAAKNSFLIAPAATLVATSLGTLAAVGLA
                     RTSFPFKGLLMSLLIAPMVVPIVVVGVSTYLFFAPLGLADSYTALIIVHAALGAPFVL
                     TTVLATLQGFDQNLVRASLGLGESPPRTFFRITLPVIAPGVVSGALFAFATSFDEVVV
                     VLFLAGPEQLTLPRQMFNGIRENISPTIAAVATLLILFTTALLLALEWLRGRRR"
     gene            complement(2264..3532)
                     /locus_tag="VIN75_09545"
     CDS             complement(2264..3532)
                     /locus_tag="VIN75_09545"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974449.1"
                     /translation="MTAAIAAPPTPAADSAALRRSLARASRRRKWRAFALTLPLLLFL
                     LLTFLVPIAALLKRAVENPEVARALPRTVVVLSDWTRADVPRPEAFSAIVADLGALSD
                     SADAGALARRLNSEKPGARSLIMGTYRALPLEGTSAATPPAEIRDKLLALDARWGETA
                     YWEAIAKNGSRWTPDYLLTAVDLRRDAHGAIERVDPDQRVFGGILLRTFGISALVTLW
                     CLALGYPLAYWLSTLPPRRANVLMIVVLVPFWTSILVRVAAWIVLLQSEGLVNHALES
                     LHLIDKPLALLFNRTGVVIAMTHILLPFMILPLYSVMTGVPPTYLRAAVSLGSTPLAA
                     FVRVYVPQTYAGIGAGALLVFILSIGYYVTPALLGGPTDQMLSYYIAQYTNVDVNWGM
                     ACALGALLLAATLVLYAVYRRVTRTELSLG"
     gene            complement(3592..4623)
                     /locus_tag="VIN75_09550"
     CDS             complement(3592..4623)
                     /locus_tag="VIN75_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974450.1"
                     /translation="MRKSTCIAAAMAALTCLPALAQKQLTVVNFGGANANAQKKAFYE
                     PYEKTGTKVVAVEYNGEQAKIKVMVDSKNVTWDAVEVESPDVARGCDEGLFEKLDWSK
                     IGNKADFVPAAVTDCGIGIFVWSTVMAWDGDKLKAGPKTWADFWDTKKFPGKRGLRKG
                     ARYNLEFALMADGVKPADVYKVLATKEGADRAFRKLGELKANIQWWEAGAQPPQFLVA
                     GDVVMTTVFNGRIDAANREGKHLQIGWVGGIYDLDYWVIPKGDPNKDAALKFIAFAST
                     ADAQAEYARNIAYGPTNKHALAKLDPKTLANLPTSPANAKDALQFNLKFWADQGEDLE
                     KRFASWATS"
     gene            complement(4668..5786)
                     /locus_tag="VIN75_09555"
     CDS             complement(4668..5786)
                     /locus_tag="VIN75_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974451.1"
                     /translation="MPAAPSPALPTAAAQPLVRFAGVQKTYDGVQLVVRQLDLDIRRG
                     EFLTLLGPSGSGKTTTLMMLAGFESPTAGEILLEGRPITRTPPHKRDFGMVFQNYALF
                     PHMTVGQNVAYPLTVRKVPAVRRDAQVAKALAMVRLEGLSDRFPSQLSGGQQQRVALA
                     RALVFEPQLVLMDEPLGALDKQLREHMQIELKELHRQLGVTFVFVTHDQGEALTMSDR
                     VAVFNNGVIQQIDAADRMYETPANRFVAGFIGDGTMLKGTLAGTAGGRCFFALPDGRR
                     LAGVDVNGAAAAAPVVACIRPERLVAHAAPPAAQDNVLRAQVRSIVYFGDHLRLMCHV
                     ADGQEPAVAKLALSTAAIPRPGDDVWLEFPSDLTRVYT"
     gene            complement(5966..>6145)
                     /locus_tag="VIN75_09560"
     CDS             complement(5966..>6145)
                     /locus_tag="VIN75_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8974452.1"
                     /translation="GLAAAIAWRFMQIKLDVPIDAADCPALAAHSARVEAFPAFAATP
                     AEDAANVSTPGYRAP"
BASE COUNT          949 a         2146 c         2168 g          882 t
ORIGIN      
        1 atcgagctgg ccggccacca gagctattgc aacgtctcga tctcggtcaa gcagttcggc
       61 ctgatcgtgg gcagcgtcgg ccaccagctg ggcgacgcgc tgctgtgcca gatcgccgcg
      121 cgcctggacc acgtcgtgcg cggcttcgaa ggccggctgt accgcgtcga cggcgtcacg
      181 ttcggcgcgc tgctgcagtt cggccacgac cccaagcgcc tggccggcat cctggaggcg
      241 ctcacgcggg cgatgggcgc gccgttcgag atcggcaacc aggacctgat cgtctcgatc
      301 ggcgtgggcg cgtccgagta ccccaaggac gccggcaccg ccgacgaggt cgcgcggctg
      361 gccgagctcg ccaagctcca ggcgcacgcc gacccgggca acagcttcct ggtctacgac
      421 tcgcgcatcg gcgaggtcca gcgcgacaag ctgcgcatgg aaagccgcat gctcaaggcg
      481 ctggagcacg gcgacttcga gctgttctac cagggccagc gcgggctgcc cgacggccgc
      541 atcaagggct tcgaggcgct gctgcgctgg cgcgacggcg acaagatgat ctcgcccgcg
      601 gacttcatcc cgctggccga ggagacgggc ctgatcgtgc gcatcggcac ctgggtgctg
      661 gaggaggcct gccgccaggc gcggtcgtgg gccgacacgg gcgccggccg gccgatcgtg
      721 gccgtcaaca tctcgccgcg ccagttcaac caccccgact tcctggccac cgtgcgcgat
      781 accctggccc gtaccggcgt cgacccgcgc cagatcgagc tcgagatcac cgagggcagc
      841 gtcatgaacg acgccgaggc ctcgatcgcg cagctgcatg cgctgcgcga cctgggcctg
      901 cacctggcca tcgacgactt cggcaccggc tacgcgagcc tgtcctacct gcgccgcttc
      961 ccgctcgacc gcctgaagat cgaccgctcc ttcgtcaagc agctcaccat gagcgaacag
     1021 gacgacacca tcgtgcgcac cgtcatcgag ctggcgcaca cgctggggct gtcggtcacc
     1081 gccgagggcg tggagacgat cgagcaggaa tcggtgctgc agggctgggg ctgcgacgtc
     1141 atccagggct tcctgcgctt ccgcccggcg ccggccgacg cggccaccgg gctgctggaa
     1201 aaggacaagc aggaagcgct gcaggccgcc tgacgctgcg cacctcgcag ggcaatgctg
     1261 gggtcaggtc ttgaatgttg cgcgggaggc ctcgcccgga tggggtcagg tcttgaatct
     1321 tgcacggcag cgtcttgccc gaagagcgct atccgaacgt gcaagattca agacctgacc
     1381 ccattctcga cagtctcgcc gcgcaacatt caagacctga ccccacctag cggaccccac
     1441 ctagcgtcgc cgcccgcgca gccactccag cgccagcagc agcgccgtgg tgaacaggat
     1501 cagcagcgtc gcgaccgcgg cgatcgtcgg cgagatgttc tcgcggatgc cgttgaacat
     1561 ctggcgcggc agcgtcagct gctccgggcc ggccaggaac agcaccacca ccacctcgtc
     1621 gaacgacgtg gcgaacgcga acagcgcgcc cgagacgacg cccggcgcga tcaccggcag
     1681 cgtgatgcgg aagaacgtgc gcggcggact ctcgccaagg ccgaggctgg cgcgcaccag
     1741 gttctggtcg aagccctgca gcgtggcgag cacggtggtc aggacgaacg gcgcgccgag
     1801 cgccgcgtgc acgatgatca gcgcggtgta gctgtcggcc aggcccagcg gcgcgaagaa
     1861 caggtaggtg ctgacgccca ccacgacgat gggcacgacc atcggcgcga tcagcaggct
     1921 catcagcagg cccttgaacg ggaacgacgt gcgcgccagg cccaccgccg cgagcgtgcc
     1981 caggctcgtg gccaccagcg tggccgccgg cgcgatcagg aagctgttct tcgcggcgcg
     2041 cgcccactcc gccgagccga acaggttctg gtaccacttg gtcgagaacg ccgggatggg
     2101 gtagaccagg aacgagctgt cgctgaacga cagcggcacg atcacgagga tgggcagcag
     2161 gaggaagccg agcaccgcca cgcagaacac gcgcagcgcc caccagccgg ccttgtccag
     2221 cggcgtcgcg taggcgggga actcgggcag ccacttcatc gcgtcatccc aggctcagct
     2281 cggtccgggt gacgcggcga tagaccgcgt acagcaccag cgtggccgcc agcagcagcg
     2341 caccgagcgc gcaggccatg ccccagttga cgtcgacgtt ggtgtactgc gcgatgtagt
     2401 agctgagcat ctggtcggtg gggccgccca gcagcgcggg ggtcacgtag tagccgatgc
     2461 tcaggatgaa gaccagcagc gcgccggcgc cgatgcccgc gtacgtctgc ggcacgtaca
     2521 cgcgcacgaa tgcggccagc ggcgtgctgc cgagggacac cgccgcgcgc aggtaggtgg
     2581 gcggcacgcc ggtcatcacg ctgtacagcg gcaggatcat gaacggcagc aggatgtgcg
     2641 tcatcgcgat cacgacgccg gtgcggttga acagcagcgc gagcggcttg tcgatcaggt
     2701 gcaggctctc cagcgcgtgg ttgaccagcc cctcggactg cagcagcacg atccaggcgg
     2761 cgacgcgcac gaggatggac gtccagaaag gcaccagcac cacgatcatc agcacgttcg
     2821 cgcgccgcgg tggcagcgtg ctgagccagt aggccagggg gtatccgagc gccaggcacc
     2881 acagcgtcac gagcgcactg atgccgaagg tgcgcagcag gatgcccccg aacacgcgct
     2941 ggtccggatc gacgcgctcg atcgcgccgt gcgcgtcgcg ccgcaggtcg accgccgtca
     3001 gcaggtagtc gggcgtccag cgcgagccgt tcttggcgat cgcctcccag taggcggtct
     3061 cgccccagcg ggcgtcgagc gcgagcagct tgtcgcggat ctcggccggc ggcgtcgcgg
     3121 cgctcgtgcc ttccagcggc agcgcgcggt acgtgcccat gatcagcgag cgcgcgccgg
     3181 gcttctcgct gttcaggcgc cgcgccagcg cgccggcgtc ggcgctgtcg gacagcgcgc
     3241 cgaggtcggc cacgattgcc gagaaggcct cgggccgcgg tacgtcggca cgcgtccaat
     3301 cggacaacac gaccaccgtg cgcggcagcg cccgcgccac ctccgggttc tcgacggcgc
     3361 gcttgagcag cgcggcgatc ggcaccagga aggtgagcag caggaacagc agcagcggca
     3421 acgtcagcgc gaacgcccgc cacttgcggc ggcggctcgc gcgcgcgagc gagcgccgca
     3481 gcgccgcgct gtcggcggcg ggcgtcgggg gggcggcgat ggcagcggtc atgcggggct
     3541 cgcgggcggc ggcgcgcggc ccgggacgcg ggccgcggcc tcgtgcccac ctcagctggt
     3601 ggcccaggac gcgaagcgct tctcgagatc ctcgccctgg tcggcccaga acttcaggtt
     3661 gaactgcagc gcatccttgg cgttggcggg cgacgttggc aggttggcca gcgtcttcgg
     3721 gtccagcttg gccagcgcgt gcttgttggt cgggccgtag gcgatgttcc tggcgtactc
     3781 cgcctgcgcg tcggccgtgc tcgcgaacgc gatgaacttc agcgccgcgt ccttgttcgg
     3841 gtcgcccttg gggatcaccc agtagtcgag gtcgtagatg ccgcccaccc agccgatctg
     3901 caggtgcttg ccctcgcggt tggcggcgtc gatgcggccg ttgaacaccg tggtcatcac
     3961 gacgtcgccc gccaccagga actgcggcgg ctgggcgccg gcctcccacc actggatgtt
     4021 ggccttgagc tcgccgagct tcctgaacgc gcggtcggcg ccctccttcg tggcgagcac
     4081 cttgtagacg tccgcgggct tgacgccgtc ggccatcagc gcgaactcca ggttgtagcg
     4141 cgcgcccttg cgcagtccgc gcttgccggg gaatttcttc gtgtcccaga agtcggccca
     4201 ggtcttcggg ccggccttga gcttgtcgcc gtcccaggcc atgacggtgg accacacgaa
     4261 gatgccgatg ccgcagtcgg tgacggccgc cggcacgaag tcggccttgt tgccgatctt
     4321 gctccagtcg agcttctcga acaggccctc gtcgcagccc cgcgcgacgt cgggcgactc
     4381 cacctccacg gcgtcccagg tgacgttctt gctgtccacc atcaccttga tcttggcctg
     4441 ctcgccgttg tattcgacgg ccaccacctt ggtgcccgtc ttctcgtacg gctcgtagaa
     4501 cgccttcttc tgcgcgttgg cgttggcccc gccgaagttc acgacggtca gctgcttctg
     4561 ggccagggcg ggcaggcacg tgagggcggc cattgccgcg gcgatgcagg tcgatttgcg
     4621 catgggactc ttcctccgga ctcgggtggt gaaggcctgg acgcgcgtca cgtgtagacg
     4681 cgcgtcaggt cgctggggaa ttcgagccag acgtcgtcgc cgggccgcgg gatcgcggcg
     4741 gtcgacagcg ccagcttcgc cacggccggc tcctggccgt ccgcgacatg gcacatgagg
     4801 cgaagatgat cgccgaagta gacgatgctg cgcacctgtg cgcgcagcac gttgtcctgc
     4861 gcggccggcg gcgcggcatg cgcgacgagg cgctcgggcc ggatgcatgc gacgaccggc
     4921 gcagcggcgg ccgcgccgtt gacgtcgacg ccggccagcc ggcggccgtc cggcagcgcg
     4981 aagaagcagc ggccaccggc ggtcccggcc aacgtgccct tcagcatcgt cccgtcgccg
     5041 atgaagcccg cgacgaagcg gttggcgggc gtctcgtaca tgcggtccgc ggcatcgatc
     5101 tgctggatca cgccgttgtt gaagacggcg acgcggtcgc tcatcgtcag cgcctcgccc
     5161 tggtcgtggg tgacgaacac gaacgtgacg cccagctgcc ggtgcagctc cttgagctcg
     5221 atctgcatgt gctcgcgcag ctgcttgtcg agcgcgccca gcggctcgtc catcagcacc
     5281 agctgcggct cgaagacgag cgcgcgcgcc agcgccacgc gctgctgctg gccgcccgag
     5341 agctgcgacg ggaagcggtc ggacagtccc tcgagccgaa ccatcgcgag cgccttggcc
     5401 acctgcgcat cgcgccgcac cgccggcacc ttgcgcaccg tcagcggata ggccacgttc
     5461 tgccccaccg tcatgtgcgg gaacagcgcg tagttctgga acaccatgcc gaagtcgcgc
     5521 ttgtgcggcg gcgtgcgcgt gatcggtcgg ccttcgagca ggatctcgcc cgcggtgggc
     5581 gattcgaacc cggccagcat catcagcgtg gtcgtcttgc cggagcccga cggcccgagc
     5641 agcgtgagga actcgccgcg gcggatgtcc agatcgagct ggcgcaccac cagctgcacg
     5701 ccgtcgtagg tcttctgcac gccggcgaag cgcaccagcg gttgcgcggc ggcggtcggc
     5761 agcgccgggg acggggcggc gggcatgcgg atgggggcct cctgggtcgc ggcgaccgca
     5821 ggcggccgcg tcgaaaaccg gacatgcagg cgtcggcgat gccgaaaccc ggacacgccg
     5881 aatatagccc gtgtgccgcg ccgcggccta tacggcgttg ctgcgaagcc tcgcccgcgc
     5941 tcgcgcgcgc ggggccacgc gccgttcaag gcgcgcggta gccgggcgtc gacacgttcg
     6001 cggcatcctc cgccggcgtt gccgcgaacg ccgggaaggc ctcgacgcgc gccgaatgcg
     6061 cggcgagcgc cgggcaatcg gcagcgtcga tcggcacgtc cagcttgatc tgcatgaagc
     6121 gccacgcgat ggccgcggcc aggcc
//
LOCUS       DATCRO010000348         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_881413, whole genome shotgun sequence.
ACCESSION   DATCRO010000348 DATCRO010000000
VERSION     DATCRO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881413"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..644)
                     /locus_tag="VIN75_09565"
     CDS             complement(192..644)
                     /locus_tag="VIN75_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEY8974453.1"
                     /translation="MATYGKKSGDKVERAMHERKEGTLKSGRSGKKVTSGKQAIAIGL
                     SEARAEGDKVPAKKSSARKTTAKKTTAARKSPARKSAETKTAAKKAPARKGTAARKAP
                     AKKAPAKKAPARKAAAKKSTARKSAAKKAPARKTPARKAAGKTTKKSS"
     gene            complement(699..2330)
                     /locus_tag="VIN75_09570"
     CDS             complement(699..2330)
                     /locus_tag="VIN75_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8974454.1"
                     /translation="MPTSSPPTAPATPPRTLRARLVDLLALLWMVLLILPAASSTSST
                     APWDIDPWMLFGLSCWLIVAARLVLPGRWFFPLTLPVALLGVACMGADFLRHADLLDL
                     GTQWRTFSAQEVRTVLEPYALATALALAVLAAWCVACARAPDQRRGGRRLLAAVAVVT
                     AALAVMVPGVSWVRAWPLNGVLVAASALAHSDRLLARLQPALSRDDPRDPHATWHGRA
                     TNTAARQTLVFIVGESVRADYLRECGGPDKVRAVAADALVACDVTSGSDATHTSVPLL
                     ISRDMPGRPYRVSADATFLRAFEEAGFETHWIGDQGKSLAWPDAQFQAYPEYRTSDEA
                     ALGPPLAQALARPAPRKALVLHAYNAHDGYCGRFDPATAPYKVDCARLNAVTGPADAA
                     ALRLGYADAIDASIGWIDGVIDTLRREPGEAFLLFTPDHGENLYDDSRQLYEHALRTP
                     TRWDTHVPAIFWANAAWRQAHPRQWANLQAQVREPLMHADMVPTFLAAGGVRYDDARP
                     LAIDLLDARVPRRTRTIERGLGRVVDWDTLVREAR"
     gene            complement(2559..3257)
                     /locus_tag="VIN75_09575"
     CDS             complement(2559..3257)
                     /locus_tag="VIN75_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEY8974455.1"
                     /translation="MRQLIAAVTLAAALAPAAHATNFFDGFDGTGGPNGGNWQTSNWG
                     NGSPFGCTFAYSEVWLAGNSNLVLNVNSSVPSAIKCSEIRTWQSFTYGKFIVRMQPGT
                     IQGGDSSFFLYTGTAGTPSHNEIDIEFINGGRTLHTNVWTADRQNYQQYAVSTGWRTI
                     GFEWRPTYVRWFNVSDSGVETEFRRVNTTISAPMQLMLNHWVGDNSAGARSFVGQYNG
                     GGGAAYYDWVKVSD"
     gene            complement(3290..4048)
                     /locus_tag="VIN75_09580"
     CDS             complement(3290..4048)
                     /locus_tag="VIN75_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974456.1"
                     /translation="MDTTNSLARLGLLMSLAASLAMAGCGGDGSAAPAAAPTPAPAPA
                     PAPVPAPAPVPTPPPPAPTPAPAPAPTPDTAFATPTDASDTAEGGSVASYAYAATASD
                     QTAGTVSYAAGAETFSASLANAQAYAGAALRLYAPGNVAGGTAAPMDASAFKQLRLHL
                     ASTTDGTLTIKLQPSPIASDGCVPTAQAVVTSTLSEFVIDLTDATFAVPSYCTAPSQT
                     LHQMLAGLYAVDVINDAAGAGNHDVVVGSLSLVP"
BASE COUNT          549 a         1487 c         1619 g          649 t
ORIGIN      
        1 tgcgtgccag cgagtcgcgc gagtcgcgca tccccgcgct ggccctgacc gccttcgcac
       61 gcgccgagga tcgcaagcgc gccctcatcg ccggcttcca ggcgcacctg gccaagccct
      121 tcgacgtggc ggagctggtg ctgctggtgg ccgacctggt gggccggacc tgaggcccgg
      181 cccaccgcgg gtcaggagga tttcttcgtc gtcttgcccg cggccttcct ggccggcgtc
      241 ttccgggcgg gcgccttctt cgcggcgctc ttgcgggccg tcgacttctt cgcggccgcc
      301 ttcctggccg gcgccttctt ggcgggagcc ttcttcgcgg gagccttcct ggccgccgtg
      361 cccttgcgcg cgggggcctt cttggcggcc gtcttcgtct cggccgactt gcgggccggc
      421 gacttcctgg cggcggtcgt cttcttggcg gtcgtcttcc tggccgacga cttcttcgcc
      481 ggcaccttgt cgccttcggc gcgcgcctcc gacagcccga tcgcgatcgc ctgcttgccg
      541 ctggtcacct tcttgcccga gcggccgctc ttgagcgtgc cttccttgcg ttcgtgcatc
      601 gccctttcga ccttgtcgcc ggacttcttg ccatacgttg ccatacgtgt ctccgttgga
      661 agtgacgcgc cgcgcgggca agcgccattc ccagcggctc agcgcgcctc gcgcacgagc
      721 gtgtcccagt cgacgacgcg gcccaggccg cgctcgatgg tgcgcgtgcg ccgcggcacg
      781 cgcgcgtcca gcaggtcgat ggccagcggg cgcgcgtcgt cgtacctgac gccgccggcg
      841 gccaggaagg tcggcaccat gtccgcgtgc atcagcggct cgcgcacctg cgcctgcagg
      901 ttggcccatt gccgcggatg cgcctggcgc cacgccgcgt tggcccagaa gatcgccggc
      961 acgtgggtgt cccagcgcgt gggcgtgcgc agcgcgtgct cgtacagctg gcgcgaatcg
     1021 tcgtacaggt tctcgccgtg gtcgggcgtg aacagcagga acgcctcgcc gggctcgcgg
     1081 cgcagcgtgt cgatgacgcc gtcgatccag ccgatcgacg catcgatcgc gtcggcatag
     1141 cccagccgca gcgccgcggc gtcggccggg ccggtcaccg cgttcagccg ggcgcagtcc
     1201 accttgtacg gcgccgtggc cggatcgaag cggccgcagt agccgtcatg cgcgttgtag
     1261 gcgtgcagca cgagcgcctt gcgcggcgcc gggcgcgcga gggcctgcgc cagcggcggg
     1321 ccgagcgcgg cctcgtccga ggtgcggtac tcggggtagg cctggaactg cgcatcgggc
     1381 cacgcgaggc tcttgccctg gtcgccgatc cagtgcgtct cgaagccggc ctcctcgaac
     1441 gcgcgcagga acgtcgcgtc ggccgacacg cggtacggcc ggccgggcat gtcgcgcgag
     1501 atcagcagcg gcaccgaggt gtgcgtcgcg tccgaaccgg acgtgacgtc gcacgcgacc
     1561 agcgcgtcgg cggccacggc gcgcaccttg tccgggccgc cgcattcgcg caggtagtcg
     1621 gcgcgcacgc tctcgccgac gatgaagacg agcgtctggc gcgccgccgt gttcgtcgcg
     1681 cggccgtgcc aggtggcgtg cgggtcgcgc ggatcgtcac gcgacagcgc cggctgcagc
     1741 ctggcgagca ggcggtcgga gtgcgcgagc gcggaggcgg cgacgagcac gccgttcagc
     1801 ggccacgccc gcacccagga cacgcccggc accatgaccg ccagcgccgc ggtcacgacc
     1861 gccaccgcgg ccagcaggcg gcggccgccg cgccgctggt cgggcgcgcg ggcgcaggcg
     1921 acgcaccagg cggcgagcac cgccagcgcc agcgcggtcg cgagcgcgta cggctccagc
     1981 accgtgcgca cttcctgcgc cgagaacgtc cgccactgcg tcccgaggtc cagcaggtcg
     2041 gcgtggcgca ggaagtcggc gcccatgcag gcgacgccca gcagcgccac gggcagcgtc
     2101 agcgggaaga accaccggcc cggcaacacg agccgcgccg cgacgatgag ccagcacgac
     2161 aggccgaaca gcatccacgg atcgatgtcc cacggcgcgg tcgacgacgt ggacgaggct
     2221 gccggcagga tcagcagcac catccacagc agggccagca ggtcgacgag gcgcgcgcgc
     2281 agcgtgcgcg gcggcgtggc gggagcggtc ggcggtgaag aggtcggcat cggtcgggcg
     2341 gcgcgcggtg gctcgtcgcg cgccggccga tcgccggcgg ggccacggcc gcgcgggcga
     2401 ttgtcgtcga tcctctcgac gactccgccg cggccgggca gtcccgggat tcacgccgcg
     2461 ggcagcgcgc cctccggggt ggatcctgcg actccgcttc gcttcgcgca gatgacggcg
     2521 tgtgcgcatc gcgtgacgcg acgcacgcct atgcgcgctc agtcgctgac cttcacccag
     2581 tcgtagtacg ccgccccgcc cccgccgttg tactgcccga cgaagctcct ggcgccggcg
     2641 gagttgtcgc cgacccagtg gttgagcatc agctgcatgg gcgccgagat ggtcgtgttg
     2701 acccggcgga actcggtctc cacgccgctg tcggacacgt tgaaccagcg cacgtaggtc
     2761 ggccgccatt cgaagccgat ggtgcgccag ccggtggaga ccgcgtactg ctggtagttc
     2821 tgcctgtcgg ccgtccacac gttggtgtgc agcgtgcggc cgccgttgat gaactcgatg
     2881 tcgatctcgt tgtggctggg cgtgccggcg gtgccggtgt agaggaagaa cgagctgtcg
     2941 ccgccctgga tggtgcccgg ctgcatgcgc acgatgaact tgccgtacgt gaagctctgc
     3001 caagtgcgga tctccgagca cttgatggcc gacggcaccg agctgttgac gttgaggacg
     3061 aggttgctgt tgcccgccag ccagacctcg ctgtaggcga aggtgcaacc gaacggggaa
     3121 ccgttgcccc agttggaggt ctgccagttc ccgccgttgg gaccgccggt accgtcgaag
     3181 ccgtcgaaga agttggtggc atgcgcggcg ggcgcgagag cggcggccag ggtcactgcc
     3241 gcgatgagtt gtcgcatgat gtctccagtc ttgtcgttga tgtgtgcgat cagggaacga
     3301 gcgagaggct gccgacgacg acgtcgtggt tgcccgcgcc ggcggcgtcg ttgatgacgt
     3361 cgacggcata gagcccggcc agcatctggt gcagcgtctg cgacggcgcg gtgcagtagc
     3421 tcggcaccgc gaacgtcgcg tcggtcaggt cgatgacgaa ctccgacagc gtggacgtca
     3481 cgaccgcctg cgcggtggga acgcagccgt ccgacgcgat cggcgacggc tgcagcttga
     3541 tcgtgagcgt gccgtcggtg gtcgaggcca ggtgcaggcg cagctgcttg aaggcagacg
     3601 cgtccatcgg cgcggcggtc ccgccggcga cgttgccagg ggcatacagc cgcagcgccg
     3661 cgccggcgta ggcctgggcg ttcgcgagtg acgcggagaa cgtctcggcg ccggcggcgt
     3721 agctcaccgt gccggcggtc tggtcgctcg ccgtggccgc ataggcatag gacgcgaccg
     3781 agccgccctc ggcggtgtcg ctcgcgtccg tcggggtggc gaaggcggtg tcgggcgtcg
     3841 gggccggcgc gggggcaggt gtcggcgccg gcgggggcgg cgtcgggacc ggtgccgggg
     3901 cggggacggg tgcaggtgcc ggcgcgggtg cgggcgtggg cgccgcggcg ggcgcagcgc
     3961 tgccgtcgcc gccgcatccg gccatcgcca gcgaggcggc gaggctcatg agcagtccca
     4021 ggcgcgccag cgagttcgtc gtatccatcg tcaatgtctc cgtagtgggt ggctagcgtc
     4081 gtgatcgtgc cgacgcgggc cccggcgcac cggtgcgcgc ttgcggagga cttgatggat
     4141 cttgcgaact tgcgggcgcg ggtctcagaa gctgtgaatg aatccggcgc gtccgagcgg
     4201 cgcgccaaaa cgcgtctgat cgaggcgcaa agcacagacg tggctcgggc cacggcgagc
     4261 atttgcaacg acgagcaggc gtgtcttggc ctgccgagcg ggcg
//
LOCUS       DATCRO010000349         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_15202, whole genome shotgun sequence.
ACCESSION   DATCRO010000349 DATCRO010000000
VERSION     DATCRO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15202"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VIN75_09585"
     CDS             <1..274
                     /locus_tag="VIN75_09585"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY8974457.1"
                     /translation="TTTSTNHAPTVTAATLSVKAGAYFATRVTAKDADGDALTFSVGG
                     MPSGLTLSSSGMLSGTTASKGTFHVTVTAHDTHGATGSAVITLVIA"
     gene            358..1398
                     /locus_tag="VIN75_09590"
     CDS             358..1398
                     /locus_tag="VIN75_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8974458.1"
                     /translation="MPRMDDAPARAKRPRRGSGAVTLHDVARLAQVAPMTASRALNTP
                     ARVSPEVLVRVREAVKATGYVRNQAAGALASTRSRLVAAVVPTLAGPVFLPTIQALTA
                     ALADHGYQLMLGQSGYVDSREDALLDAIIGRRPDGIVLTGTVHSPDGRQRLQAAGIPV
                     VETWDLAPDPLDMLVGFSHEAVGRAVAQFLQARGRRRLAVIAGDDERSRRRHEAFRAA
                     AAERGLPEAAVVTVPAPTTLRSGRSALVELLRRPGGVDAVFCSSDLLALGVLTEARVR
                     GLDVPGQLAVVGFGDHEFAADLEPALTTVRIDGGAIGRQAARFIVDRAEGREVAERVV
                     DVGFSIVDRTSA"
     gene            1482..2816
                     /gene="gudD"
                     /locus_tag="VIN75_09595"
     CDS             1482..2816
                     /gene="gudD"
                     /locus_tag="VIN75_09595"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEY8974459.1"
                     /translation="MSLSALPTIADLRVIPVAGRDSMLLNLSGAHAPFFTRNLLILTD
                     TSGRTGIGEVPGGEKIRQTLEDAGALVVGEPLGAHLRLLDEVRRRFADRDSGGRGLQT
                     FDLRTTIHAVTALESALLDLLGQHLGVPVAALLGEGQQRDAVDALGYLFYIGDRMRSG
                     LPYASDPDAPDAWSRLRHETALTPEAIVRLAEAAHERYGFEDFKLKGGVLRGDEEVLA
                     IRALHERFPQARITLDPNGAWSLQESVRLLRDLRGVLAYAEDPCGAEAGFSGREVMAE
                     FRRATGLPTATNMIATDWRQLAHALSLQSVDIPLADPHFWTMAGSVRVAQTCRDWGLT
                     WGSHSNNHFDVSLAMFTHVAAAAPGRVTAIDTHWIWQDGQRLTKVPLRIAGGKLRVPQ
                     APGLGVELDMAEVEKAHALYRQHGLGARDDAIAMQSLMPGWTFDPKRPALEH"
     gene            2885..>3658
                     /locus_tag="VIN75_09600"
     CDS             2885..>3658
                     /locus_tag="VIN75_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8974460.1"
                     /translation="MGMSYLPYEPQQQALLPQALQDWLPEGHLAYFISDTVDSLELQA
                     FYARYEGGGSRNQPFHPAMMVKVLVYAYATGVFSSRKIARKLHEDVAFRVLAADNFPA
                     HRTISDFRAFHLKELGELFVQVVRLAREMGLVKLGTIAVDGTKVKANASRHKAMSYGH
                     MLKVEAELKAQVAALLQRAKAADEAEKNEPEIDIPAEIARRQDRLDAIAQARARLEQR
                     QREAELDKGRDPDDKPRGGRYKREFGVPEDKAQDNFTDPD"
BASE COUNT          529 a         1358 c         1261 g          510 t
ORIGIN      
        1 cacgaccacc tcgacgaacc acgcgccgac ggtgacggcg gccacgctgt cggtcaaggc
       61 cggcgcgtac ttcgccaccc gcgtcaccgc caaggacgcc gacggcgacg ccctgacctt
      121 ttcggtcggc ggcatgccga gcggcctgac cctgtccagc agcggcatgc tcagcggcac
      181 cacggcgtcc aagggcacct tccacgtgac ggtcaccgcc cacgacacgc acggcgccac
      241 cggcagcgcg gtcatcacgc tggtcatcgc gtaaggcttc gcgccctccg catcgacgcc
      301 cgcctcgcgc gggcgtcgtc gtttctgccc agtgcgcaac gcggcggctc acgtaacatg
      361 ccccgcatgg atgacgcccc ggcccgcgca aagcgacccc gacgcggcag cggtgccgtg
      421 accctgcacg acgtcgcccg cctggcgcag gtcgcgccga tgacggcctc gcgcgcgctc
      481 aacacgcccg cccgcgtgtc gcccgaggtg ctcgtgcgcg tgcgcgaggc ggtcaaggcc
      541 accggctacg tgcgcaacca ggcggccggt gcgctggcct ccacgcgcag ccggctggtc
      601 gccgccgtcg tgcccacgct tgccgggccg gtgttcctgc ccaccatcca ggcgctgacc
      661 gccgcgctgg ccgaccacgg ctaccagctg atgctgggcc agagcggcta cgtcgactcg
      721 cgcgaggacg ccctgctcga cgccatcatc ggccgccggc ccgacggcat cgtgctgacc
      781 ggcaccgtgc attcgcccga tggccgccag cgcctgcagg cggccggcat cccggtcgtc
      841 gagacctggg acctggcccc cgacccgctg gacatgctgg tcggcttctc gcacgaggcc
      901 gtgggccgcg cggtcgcgca gttcctgcag gcgcgcggcc gccggcgcct cgcggtgatc
      961 gccggcgacg acgagcgctc gcggcgccgc cacgaggcct ttcgcgccgc cgccgccgag
     1021 cgcggcctgc ccgaggccgc cgtcgtcacc gtccccgcgc ccaccacctt gcgcagcggc
     1081 cgctcggcgc tggtcgagct gctgcgccgg ccggggggcg tcgacgccgt gttctgcagc
     1141 tccgacctgc tcgcgctggg cgtgctgacg gaggcccgcg tgcgcggcct ggacgtgccg
     1201 ggccaactcg ccgtggtcgg tttcggcgac cacgagttcg ccgccgacct ggaacccgcg
     1261 ctgaccaccg tgcgcatcga cggcggtgcc atcggccgcc aggcggcgcg cttcatcgtc
     1321 gaccgtgccg aggggcgcga ggtcgcggag cgggtcgtcg acgtcggctt ctccatcgtc
     1381 gatcgcacga gcgcctgagc gctgcggcct acgccaactt cgggcttgcg actcgtgatt
     1441 gttagcgcta ccatgcattc atcgctccaa cgtccgccgc catgtctctg tccgccctgc
     1501 cgaccatcgc cgacctgcgc gtcatccccg tcgcgggccg cgacagcatg ctgctcaacc
     1561 tcagcggcgc gcacgccccg ttcttcacgc gcaacctgct gatcctgacc gacacctcgg
     1621 gccgcaccgg cattggcgag gtgcccggcg gcgagaagat ccgccagacg ctggaggacg
     1681 cgggcgccct ggtggtcggc gagccgctgg gcgcgcacct gcggctgctc gacgaggtgc
     1741 ggcggcgctt cgccgaccgc gacagcggcg gccgcggcct gcagaccttc gacctgcgca
     1801 ccaccatcca cgccgtcacc gcgctcgagt cggcgctgct ggacctgctg ggccagcacc
     1861 tgggcgtgcc ggtcgccgcg ctgctgggcg aaggccagca gcgcgatgcg gtcgacgcgc
     1921 tgggctacct gttctacatc ggcgaccgca tgcgcagcgg cctgccctac gcgagcgatc
     1981 ccgacgcgcc cgacgcctgg tcccgcctgc gccacgagac ggcgttgacg cccgaggcca
     2041 tcgtgcgcct ggccgaggcc gcgcacgagc gctacggctt cgaggacttc aagctcaagg
     2101 gcggcgtgct gcgcggcgac gaggaggtgc tggcgatccg cgcgctgcac gagcgcttcc
     2161 cgcaggcgcg catcacgctc gatcccaacg gcgcgtggtc gctgcaggag tcggtgcgat
     2221 tgctgcgcga cctgcgcggc gtgctcgcct atgccgagga tccgtgcggc gccgaggccg
     2281 gcttctcggg gcgcgaggtc atggccgagt tccgccgcgc gaccggcctg ccgacggcca
     2341 ccaacatgat cgccaccgac tggcgccagc tggcgcacgc gctgtcgctg cagtcggtcg
     2401 acatcccgct ggccgatccg catttctgga cgatggccgg ctccgtgcgc gtcgcgcaga
     2461 cctgccgcga ctggggcctg acctggggct cgcactcgaa caaccatttc gacgtctcgc
     2521 tggcgatgtt cacgcacgtc gccgccgccg cgcccggccg cgtcaccgcc atcgacacgc
     2581 actggatctg gcaggacggc cagcgcctga ccaaggtgcc gctgcgcatc gccggcggca
     2641 agctgcgcgt gccccaggca cccggcctgg gcgtcgaact ggacatggcc gaggtcgaga
     2701 aggcgcatgc gctgtatcgc cagcacggac tcggcgcgcg cgacgacgcg atcgcgatgc
     2761 agtcgctcat gccgggctgg acattcgatc cgaagcggcc ggcgctcgaa cactaggagt
     2821 ctgcgcggca gaagccgact acggggtggg accgatacgc gccagggcgt gaggcgagta
     2881 gattttgggc atgagctatc tgccctacga accccagcag caggcgttac tgccgcaggc
     2941 gctgcaggac tggctgcccg aagggcatct ggcctacttc atcagcgaca cggtcgacag
     3001 tctggaactg caggcgttct acgcgcgcta cgaaggcggc ggttcgcgca accagccgtt
     3061 ccatccggca atgatggtca aggtgctggt ctacgcgtac gccaccggcg tgttcagctc
     3121 gcgcaagatc gcccgcaagc tgcacgagga cgtcgcattt cgcgtgctgg ccgccgacaa
     3181 ctttccggcg caccgcacga tcagcgactt ccgggcgttc cacttgaagg aactgggcga
     3241 gttgttcgtg caggtggttc gcctggctag agagatgggg ctggtcaagc tcggcacgat
     3301 tgcggtggac ggcaccaagg tcaaggccaa cgcgagccgc cacaaggcca tgagctacgg
     3361 gcacatgctc aaggtcgaag ccgagctgaa ggcgcaggtc gcggcgctgt tgcagcgcgc
     3421 caaggctgcc gacgaggccg agaagaacga gcccgagatc gacatccccg ccgagatcgc
     3481 gcgccgtcaa gaccggctgg acgctatcgc tcaggcgcga gccaggctgg agcagcgcca
     3541 gcgtgaggct gagctggaca agggtcgcga ccctgacgac aagccgcgtg gtggccgtta
     3601 caagcgcgag tttggcgtgc cagaggacaa ggcgcaggac aacttcaccg accccgac
//
LOCUS       DATCRO010000350         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_453710, whole genome shotgun sequence.
ACCESSION   DATCRO010000350 DATCRO010000000
VERSION     DATCRO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453710"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..480
                     /locus_tag="VIN75_09605"
     CDS             118..480
                     /locus_tag="VIN75_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY8974461.1"
                     /translation="MQIQEAVETCVTKKYADFEGRATRSEFWWFMLFCVVINTLLGVA
                     AEWAAGIFFVVMLVPSLAVGTRRLRDTGRSPWWWLLMAPPMIVIGWLVLLVFFVQRGT
                     TPTTPATPDHPGSPATPT"
     gene            complement(493..1077)
                     /gene="metW"
                     /locus_tag="VIN75_09610"
     CDS             complement(493..1077)
                     /gene="metW"
                     /locus_tag="VIN75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY8974462.1"
                     /translation="MSERRDLDIITSLVPRGSHVLDLGCGNGEVLAHLTQHHGCSGYG
                     IEIADEGVLACTQRGVNVIQLNLEEGLALFDDHSFDVVLQLDTLQHLRNTEKMLRETA
                     RVGRIGIVSFPNFAHWPNRLRIATGRMPVTRALPYEWYDTPNIRVTTHADFEVLARKN
                     GLDILESFGLQDGRVVRRRPNLMASVAVFKFQRS"
     gene            complement(1166..2410)
                     /locus_tag="VIN75_09615"
     CDS             complement(1166..2410)
                     /locus_tag="VIN75_09615"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY8974463.1"
                     /translation="MGSVGRIEPQAMHFVEPLPLTSGKQLNDYTLVYETYGTLNAARD
                     NAVLVCHALNASHHVAGVSPKGDTGWWDNLVGPGKPLDTDRFFVIGVNNPGSCFGSTG
                     PTSPNPDPSANGRVYGADFPVVTVEDWVDAQARLIERLGIAKLAAVLGGSLGGMQALA
                     WTLRYPERVAHCVAVATAPKLSAQNIAFNEVARRAIVTDPDFHGGHYTEAGTVPKRGL
                     RVARMIGHITYLSDDSMEAKFGRATRAAADNGARHHYSTQDIEFQVESYLRYQGDKFS
                     EYFDANTYLLITRALDYFDPAHAHDGDLSAAFAIARACRFLVVSFTTDWRFAPQRSRE
                     IVKALVDNRAEVSYAEIDAPHGHDAFLLDDPRYHDVMRAYFERIFDGLADATRGERRA
                     APSRPGVPLGDGAPYPAREGQS"
     regulatory      complement(2456..2533)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2547..3440
                     /locus_tag="VIN75_09620"
     CDS             2547..3440
                     /locus_tag="VIN75_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY8974464.1"
                     /translation="MSRTLTTPLRAAAAALALIAAAGLHAQETAADTAGATEPTRPRE
                     PLPLWEIGLVGIAAYQPAYPGSDQDLGRLRVLPFGIYRGSVLHADGNGIGVRALRTPR
                     WEWDVSGSGSFGSSANKVDARRGMPSIGTLVEVGPAVKINLGDLIDAKREPRLTQFEL
                     PVRAVFDVSHHFDHDGWTFEPRLSHTAWTGQGFSLVVSASTLFGDRTLNHLYYGVDTP
                     YATLDRPAYGAKAGLIATRLNASLRHRISPTLRMQYFAQLETVRGAANEDSPLVRSKQ
                     DAGIGASLIWGAWHSAEAGVE"
BASE COUNT          503 a         1227 c         1261 g          533 t
ORIGIN      
        1 agcccgcgcc cgccgattga cgcgcgcctc caccgcccgc gcgttcccat cgacagccaa
       61 cccggctacc atcccgggca gtccagggac acgagaaaag cgaagagaga gcgcgacatg
      121 cagatccagg aagccgtcga gacatgcgtc acgaagaagt acgccgactt cgagggccgg
      181 gccacgcgtt ccgagttctg gtggttcatg ctcttctgcg tcgtcatcaa cacgctgctg
      241 ggcgtggcgg ccgaatgggc cgccggcatc ttcttcgtgg tgatgctggt gcccagcctg
      301 gcggtgggca cccggcgcct gcgcgacacc gggcgcagcc cgtggtggtg gctgctgatg
      361 gcgccgccga tgatcgtgat cggctggctc gtgctgctcg tgttcttcgt gcagcgcggc
      421 acgacgccga ccacgccggc cacgcccgac catccgggca gcccggccac cccgacctga
      481 acggcccgcg gctcagctgc gctggaactt gaacacggcc acgctggcca tcaggttcgg
      541 ccgccgccgc acgacgcgcc cgtcctgcag gccgaacgat tcgaggatgt cgagcccgtt
      601 cttgcgcgcc agcacctcga agtcggcatg cgtcgtcact cggatgttgg gcgtgtcgta
      661 ccactcgtag ggcagcgcgc gcgtgacggg catgcggccc gtggcgatgc gcagccggtt
      721 gggccagtgc gcgaagttcg ggaagctgac gatgccgatg cggcccacgc gcgccgtctc
      781 gcgcagcatc ttctcggtgt tgcgcaggtg ctgcagcgtg tccagctgca ggacgacgtc
      841 gaagctgtgg tcgtcgaaca gcgccagccc ttcctcgagg ttgagctgga tgacgttgac
      901 gccgcgttgc gtgcacgcga gcacgccctc gtcggcgatc tcgatgccgt agccgctgca
      961 gccgtggtgc tgcgtcaggt gcgcgagcac ctcgccgttg ccgcagccga ggtccagcac
     1021 atgcgagccg cgcgggacga gactcgtgat gatgtccagg tcgcgtcgtt cgctcatagt
     1081 gactgcccct cccgcgccgg gtacggcgct ccgtccccca aggggaccgc gggccggctt
     1141 ggggcggccc ggcgctcgtc ccgcgtcatg actgcccctc ccgcgccggg tacggcgctc
     1201 cgtcccccaa gggaaccccg ggccggcttg gggcggcccg gcgctcgccg cgcgtcgcgt
     1261 ccgcaagccc gtcgaagatg cgctcgaagt acgcgcgcat cacgtcgtgg tagcgcgggt
     1321 cgtccagcag gaaggcgtcg tggccgtgcg gcgcgtcgat ctcggcgtag gagacctcgg
     1381 cgcggttgtc gaccagcgcc ttgacgatct cgcgcgagcg ctgcggggcg aagcgccagt
     1441 cggtggtgaa gctcacgacg aggaaacggc aggcgcgggc gatcgcgaac gcggccgaca
     1501 ggtcgccgtc gtgcgcgtgc gcagggtcga agtagtcgag cgcgcgggtg atcagcaggt
     1561 aggtgttggc gtcgaagtac tcgctgaact tgtcgccctg gtagcgcagg tagctctcga
     1621 cctggaactc gatgtcctgc gtcgagtaat gatggcgcgc gccgttgtcc gcggcggcgc
     1681 gggtggcgcg gccgaacttg gcctccatcg agtcgtccga caggtaggtg atgtggccga
     1741 tcatgcgcgc cacgcgcagg ccgcgcttgg gcacggtgcc ggcctcggtg tagtggccgc
     1801 cgtggaagtc ggggtcggtg acgatcgcgc ggcgcgcgac ctcgttgaac gcgatgttct
     1861 gcgccgacag cttgggcgcc gtggccaccg ccacgcagtg cgcgacgcgc tcggggtagc
     1921 gcagcgtcca cgcgagcgcc tgcatgccgc ccaggctgcc gcccaggacg gcggcgagct
     1981 tggcgatgcc caggcgctcg atcaaccgcg cctgcgcgtc cacccagtcc tccacggtga
     2041 cgacggggaa gtcggcgccg tagacgcggc cgttggccga cggatccggg ttgggcgacg
     2101 tgggcccggt cgagccgaag cacgaacccg ggttgttgac gccgatgacg aagaagcggt
     2161 cggtgtccag cggcttgccg gggcccacca ggttgtccca ccagccggtg tcgcccttgg
     2221 gcgagacgcc cgccacgtgg tggctcgcgt tgagcgcgtg gcacacgagc acggcgttgt
     2281 cgcgcgcggc gttgagcgtg ccgtaggtct cgtagaccag cgtgtagtcg ttcaattgct
     2341 tgccgctggt gagcggcagc ggctcgacga agtgcatcgc ctgcggctcg atacggccga
     2401 cggaacccat ctcggcaccc tttcaggtca cggtcatccg ggggaaaagc tcggacaccg
     2461 cgcggggcgc ccctctttag cgatatttgt agagcgcccg caagccggaa atcaaatcgg
     2521 cgctggcctg attatagtgg cccgccatga gccgaaccct gaccacgccc cttcgcgccg
     2581 ccgcggctgc gctcgcactc atcgccgccg ccggcctgca tgcgcaggag acggccgccg
     2641 acaccgccgg cgccacggag cccacgcgcc cccgggagcc gctgccgctg tgggagatcg
     2701 gcctggtggg catcgccgca taccagccgg cgtatccggg ctccgaccag gacctcgggc
     2761 ggttgcgcgt attgccgttc ggcatctacc gcggctccgt gctgcacgcc gacggcaacg
     2821 gcatcggcgt gcgcgcgctg cgcacgccgc gctgggaatg ggacgtcagc gggtcgggct
     2881 cgttcggctc gtcggccaac aaggtcgacg cgcgccgcgg catgcccagc atcggcacgc
     2941 tggtggaggt cggcccggcg gtcaagatca acctgggtga cctcatcgac gccaagcgcg
     3001 agccgcggct gacgcagttc gagcttccgg tgcgcgccgt gttcgacgtc agccaccact
     3061 tcgaccacga cggctggacc ttcgagccgc gcctgtcgca cacggcctgg acgggccagg
     3121 gcttctcgct ggtcgtctcg gcgagcacgc tgttcggcga tcgcacgctc aaccacctgt
     3181 actacggcgt cgacacgccc tacgcgaccc tcgaccgccc ggcctatggc gccaaggcgg
     3241 gcctgatcgc cacgcgcctg aacgccagcc tgcgccatcg catcagcccg acgctgcgca
     3301 tgcagtactt cgcgcagctg gagacggtgc gcggcgccgc caacgaggac agcccgctgg
     3361 tgcgcagcaa gcaggacgcg ggcatcggcg cgagcctgat ctggggcgcc tggcactccg
     3421 ccgaggcggg cgtcgagtag gccccgcggt cgacgcgcgc cgcggcatgc ccagcatcgg
     3481 cacgctggtg gaggtcggcc cggcggtcaa gatcaacctg ggtg
//
LOCUS       DATCRO010000351         7628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_420414, whole genome shotgun sequence.
ACCESSION   DATCRO010000351 DATCRO010000000
VERSION     DATCRO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420414"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VIN75_09625"
     CDS             complement(<1..725)
                     /locus_tag="VIN75_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8974465.1"
                     /translation="MKKIGLAIAALACMGAAHADDDGGPAVVPYRPSVSTPAQLSTPG
                     WLELEAGGLRAGDGGAVRTTLPYTLKLAFTPDWGIRVGGDAWVHQDVGGGSRNSFGDT
                     SVVLKRRFAVSDATAWGLELGETFPTAKGDVGIGHAQTTINGIFSSDFAPGWHTDINL
                     NETHVTASNEPGGWQAGWAAAVSRNLSDAWGVVGEFSGTHQPGTPNQAQFLAAASWSE
                     SNAAVFDFGAARGLNDASPRWQV"
     gene            complement(722..1198)
                     /locus_tag="VIN75_09630"
     CDS             complement(722..1198)
                     /locus_tag="VIN75_09630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974466.1"
                     /translation="MGVRFVPFTEADFDAWLVQAIPAFALTNVRDGRWTLAESIVKSQ
                     EAHLALLPQGLATPGQFFVHLHDEAGGQRVGSLWWSEGGKGGRREAYVYNIEIDEAAR
                     RRGHARAAFAELERVAREHGMQQVGLHVFGHNTGARRLYDELGFEPTSITMRKVLR"
     gene            complement(1248..1481)
                     /locus_tag="VIN75_09635"
     CDS             complement(1248..1481)
                     /locus_tag="VIN75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEY8974467.1"
                     /translation="MKTNVTLIIAAALALAGSSSFAAGKACEELKTEIAAKLDAKNVT
                     GYTLDIVEADKVGDAKVVGTCEGGSKKITYTKK"
     gene            complement(1570..3657)
                     /locus_tag="VIN75_09640"
     CDS             complement(1570..3657)
                     /locus_tag="VIN75_09640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY8974468.1"
                     /translation="MRLTVAIDAPQYAGLGSLLTYESEQALAPGTLVRVPLGRREVTG
                     LVWDADLDALDAAMAQELADGVARPAIALKPVIEALASLPPLPADWRALAEFTARYYQ
                     RSLGEVALAVLPPELRKLDDTGLAKRVKKKRGPATTATAEPPAKPPLTAEQAAAMATL
                     GEWAASPAPPPALLFGVTGSGKTEVYLRLAEQALEAGKQALVLVPEINLTPQLEQRFA
                     ARFPGRRLVSLHSALTPAQRLQNWLAAHLGEADLVLGTRLAVFASLPRLGLVVVDEEH
                     DPSYKQQDGARYSARDLAVYRGHLAKVPVLLGSATPSLETWSHAQTGRYRQVTLAERV
                     GRGALPEVRLLDMATVPRPAPRMPPQALAPQLIAAIQERVARGEQSLLFLNRRGFAPV
                     LHCPACTWKSGCPHCSAWRVFHKGERILRCHHCGTQEPVPRACPDCGNLDIEPMGRGT
                     ERLEEQVAELLPGARVLRIDADTTRAKGTLESQLAAVHEGAVDVLVGTQMVTKGHDFR
                     RVTLVAAVNPDSALFAADFRAPERLFALLMQAGGRAGRDADIARGSQMWVQTYNPGHA
                     LFEALRKHDFAAFAATQLAERASAGLPPYAHLALLRAEARTEDVAEGFLRAARELAKA
                     YPEATEVMFYAPVPPSVAKVAGLERRQMLIECASRATLQRLLARFTPELPALKASHRG
                     IVRWAIDVDPLGI"
     gene            complement(4039..5127)
                     /gene="hemE"
                     /locus_tag="VIN75_09645"
     CDS             complement(4039..5127)
                     /gene="hemE"
                     /locus_tag="VIN75_09645"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY8974469.1"
                     /translation="MWPALKNDTFLRACLRQPTDYTPIWLMRQAGRYLPEYKATRARA
                     GSFMGLATNVDFATEVTLQPLERFPLDAAILFSDILTVPDAMGLGLSFAEGEGPRFAT
                     TVRDEAAVAKLAVPDMDKLRYVFDAVSSIRKALDGRVPLIGFSGSPWTLACYMVEGAG
                     SDDYRQVKTLMYARPDLMHRLLEVNADAVAAYLNAQIDAGAQAVMVFDSWGGVLADAA
                     FRTFSLAYTRRVLAQLQREKDGQRIPAIVFTKGGGQWLEAIADTGADVVGLDWTTDLG
                     SARRRVGDRVALQGNLDPMALFAPPEAVRAEARAALDSFGAQRDTSGRIGGHIFNLGH
                     GISQFTPPEHVAALVEAVHAHSRRHHGL"
     gene            complement(5174..5944)
                     /gene="ppk2"
                     /locus_tag="VIN75_09650"
     CDS             complement(5174..5944)
                     /gene="ppk2"
                     /locus_tag="VIN75_09650"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY8974470.1"
                     /translation="MSKKKADGHSYEDQLRPLQVELTGVMRLLQERGERVVVLFEGRD
                     TAGKGGVIKAISEKLNTRQCHIAALGVPTPREQGEWYFQRYVAYLPTAGEMTLFDRSW
                     YNRAGVEAVMGFCTPAQTDAFLKQAPVFERMLVDDGIRLFKYWLTVDQAEQEQRFEER
                     QDDPLKQWKLSPVDLQARSKYVEYGRARDRMLEATHTKWAPWTLVDFNDQKAGRLKLI
                     ANLLSRLPVATASAPEVEIPPLPGKPHKEKFRGPLKPI"
     gene            complement(6027..6356)
                     /locus_tag="VIN75_09655"
     CDS             complement(6027..6356)
                     /locus_tag="VIN75_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974471.1"
                     /translation="MSIPDMPLGGPLGDALAAAAPAATQQLQQLAAPGADAASKAAAL
                     KAAAAQAAADGASAPGGDATSAAEAAAANAASDIGSSFSDATPAGAPAVPCGCAECVA
                     AACRAAA"
     gene            complement(6353..6907)
                     /locus_tag="VIN75_09660"
     CDS             complement(6353..6907)
                     /locus_tag="VIN75_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974472.1"
                     /translation="MSARAWRARAAPTSNGAPDARARDVLHGYTVRCRPSAGRPRRAM
                     PESRGMPAGGPIRVSMKQISREQWNRLGQDSFDARMVAVIRRHHPEQAARMDFPALVG
                     AIRRQAERARGHGLADERSVATYVYTAWLMGEEFDRRIPAVAQILQDEAMAAGDKAQA
                     LTDFSRLVFRALGNGANDAGRRAA"
     gene            complement(6984..>7628)
                     /locus_tag="VIN75_09665"
     CDS             complement(6984..>7628)
                     /locus_tag="VIN75_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974473.1"
                     /translation="QGEADAAAQAAQAQAAQAQQQLADAQSQAASSADSAAGAAEAAA
                     ANATKDLGSEFSNTTPAGLPAVPCACGDCIKKAVDPAVPAIPGTPALPALPALPDLPA
                     MPAMPAMPAMPAQPAMPAMPAMPAANGLPDLPAMPAMPALPDMPALPAMPAQPALPAL
                     PAMPALPAMPALPATPAMPAMPAMPAMPAMPAMPAMPAAPAMPAMPAVPALPTF"
BASE COUNT         1011 a         2738 c         2774 g         1105 t
ORIGIN      
        1 aacacctgcc agcgcggcga cgcgtcgttc aggccgcgcg cggcgccgaa gtcgaacacc
       61 gcggcattcg attcgctcca gctcgcggcc gcgaggaact gcgcctggtt gggcgtgccc
      121 ggctggtgcg tgcccgagaa ctcgccgacc acgccccagg cgtcggacag gttgcgcgac
      181 accgcagcgg cccagcccgc ctgccacccg cccggctcgt tcgacgcggt cacgtgcgtc
      241 tcgttgaggt tgatgtcggt atgccagccc ggcgcgaaat cgctgctgaa gatgccgttg
      301 atcgtcgtct gcgcatggcc gatgcccaca tcgcccttgg cggtcgggaa cgtctcgccc
      361 agctccaggc cccaggccgt cgcgtcgctc accgcgaagc ggcgcttgag gacgaccgac
      421 gtgtcgccga agctgttccg cgatccgccg ccgacgtcct ggtgcaccca cgcgtcgccg
      481 ccgacgcgga tgccccagtc gggcgtgaac gcgagcttga gcgtgtacgg aagcgtcgtg
      541 cgcacggcgc cgccgtcgcc ggcgcgcagc ccgcccgctt ccagctccag ccagccgggg
      601 gtcgacagct gcgccggcgt cgacacgctg gggcggtacg gcacgacggc cgggccgccg
      661 tcgtcgtcgg catgcgcggc gcccatgcag gccagggccg cgatcgcaag accgatcttc
      721 ttcatcgaag aacctttctc atcgtgatgc tggtgggttc gaacccgagt tcgtcgtaga
      781 ggcggcgcgc gcccgtgttg tggccgaaca cgtgcaggcc cacctgctgc atgccgtgct
      841 cgcgcgcgac ccgttcgagc tccgcgaagg cggcccgtgc gtggccgcgg cggcgcgcgg
      901 cctcgtcgat ctcgatgttg tagacgtacg cctcgcgccg gccgcccttg ccgccctccg
      961 accaccacag cgagcccacg cgctgcccgc cggcctcgtc gtgcaggtgc acgaagaact
     1021 ggcccggcgt ggccaggccc tgcggcagca gcgcgaggtg cgcttcctgc gacttgacga
     1081 tggactcggc cagcgtccag cggccgtcgc gcacgttggt cagcgcgaag gcggggatcg
     1141 cctgcaccag ccaggcgtcg aaatcggcct cggtgaacgg gacgaagcgg acgcccatca
     1201 gtcggcggcg gccccggcca cgaccatgcg gcggcgaggc agctgcctca cttcttggtg
     1261 taggtgatct tcttggagcc gccttcgcag gtgcccacca ccttggcgtc gcccaccttg
     1321 tcggcctcga cgatgtccag cgtgtagccg gtgacgttct tggcgtcgag cttggctgcg
     1381 atctcggtct tgagttcctc gcaggccttg ccggccgcga acgaggacga gccggccagc
     1441 gcgagggcgg cggcgatgat cagcgtgaca ttggtcttca tgcgagagct ccccatcggt
     1501 gtgaaagaca gcagcgactg tactgcggcc ggctgtccga tggctctccc ggttcgagaa
     1561 atcggcgcct cagattccga gcgggtcgac gtcgatcgcc cagcgcacga tgccgcggtg
     1621 cgacgccttg agtgccggca gctccggtgt gaaccgggct agcagccgtt gcagcgtcgc
     1681 gcgcgaggcg cactcgatca gcatctggcg ccgctcgagg ccggccacct tggccaccga
     1741 cggcggcacc ggcgcataga acatgacctc ggtcgcctcg ggatacgcct tggcgagctc
     1801 gcgcgccgcg cgcaggaagc cctcggcgac gtcctcggtc ctggcctcgg ctcgcagcag
     1861 cgccaggtgc gcgtacggcg gcaggcccgc cgatgcgcgt tcggcgagct gggtggcggc
     1921 gaaggcggcg aagtcgtgct tcctgagcgc ctcgaacagc gcgtggcccg ggttgtacgt
     1981 ctgcacccac atctggctgc cgcgcgcgat gtcggcgtcg cggcccgcgc ggccgccggc
     2041 ctgcatcagc agcgcgaaca ggcgctcggg ggcgcggaag tcggcggcga acagcgcgct
     2101 gtccgggttg acggcggcca ccagcgtgac gcggcggaag tcgtgaccct tggtgaccat
     2161 ctgcgtgccc accagcacgt cgaccgcgcc ctcgtgcacc gccgccagct gcgattcgag
     2221 cgtgcccttg gcgcgcgtgg tgtcggcgtc gatgcgcagc acgcgcgcgc cgggcagcag
     2281 ctcggcgacc tgctcctcca ggcgctcggt gccacggccc atcggctcga tgtccaggtt
     2341 gccgcagtcg gggcaggcgc gcggcaccgg ctcctgcgtg ccgcaatggt ggcagcgcag
     2401 gatgcgctcg cccttgtgga acacgcgcca ggcgctgcag tgcgggcagc cgctcttcca
     2461 cgtgcaggcc gggcagtgca ggacgggcgc gaaaccccgc cggttcagga acagcagcga
     2521 ctgctcgccg cgggcgacgc gctcctggat cgcggcgatc agctgcggcg ccagcgcctg
     2581 gggcggcatg cgcggcgcgg ggcgcggcac cgtggccatg tccagcagcc ggacctcggg
     2641 cagcgcgccg cggcccacgc gctcggccag cgtcacctgg cggtagcgcc cggtctgcgc
     2701 gtgcgaccag gtctccagcg acggcgtcgc cgaccccagc agcaccggca ccttggccag
     2761 gtgcccgcga tagaccgcca ggtcgcgcgc cgagtagcgc gcgccgtcct gctgcttgta
     2821 cgacgggtcg tgctcctcgt cgacgacgac cagccccagc cgcggcagcg acgcgaatac
     2881 cgccagccgc gtgcccagga ccaggtcggc ctcgcccagg tgcgcggcga gccagttctg
     2941 cagccgctgc gccggcgtca gcgcgctgtg cagcgacacc agccgccggc cgggaaagcg
     3001 cgccgcgaag cgctgctcga gctgcggggt caggttgatc tcgggcacca gcaccagcgc
     3061 ctgcttgccg gcctccagcg cctgctcggc caagcgcagg tagacctcgg tcttgccgct
     3121 gcccgtcacg ccgaacagca gcgccggcgg cggcgccggc gacgccgccc actcgccgag
     3181 cgtggccatc gcggccgcct gctcggccgt cagaggcggc ttggccggcg gctcggccgt
     3241 ggcggtggtc gccgggccgc gcttcttctt cacgcgcttg gccagaccgg tgtcgtcgag
     3301 cttgcgcagc tcgggcggca gcaccgccag cgcgacctcg cccaggctgc gctgatagta
     3361 gcgcgccgtg aactccgcca gcgcgcgcca gtcggccggc agcggcggca ggcttgccag
     3421 cgcctcgatg accggcttca acgcgatcgc gggacgcgcc acgccatctg caagctcctg
     3481 cgccatggcc gcgtcgagcg catcgagatc cgcgtcccac accagcccgg tgacttcgcg
     3541 ccggcccagc ggcacgcgca ccagcgtgcc aggcgcaagc gcctgctcac tctcgtaggt
     3601 gagcagagag cccaggccgg cgtactgcgg cgcatcgatg gcgacggtca ggcgcatcag
     3661 gccccgcctc cggacatcga tccgagttgg gcccgaaagt tgtcaggctt ttgagctgcg
     3721 agcgataagc tcatgatccg taaggagttt tgcagtcgcc cacagtttct gtggataact
     3781 ttgtgggaaa gctggcagaa gcgccgccaa gggcttgatt cgtcgagccc ggcccttgaa
     3841 cgctcaaaat agatgcacgc ccgaaatatg ccggtggatc aaggacttgg gcctactttg
     3901 cggcgtttcc gggggggtcc gggtaccccc cgggggcaaa gttatcgctc tctgaatatt
     3961 ggggataagt caacacctga gcactcactt accggacaaa acgcgtgctt tctgcgttcg
     4021 cggtctgcaa gtggtctcct acagcccgtg gtggcggcgc gaatgcgcat gcacggcttc
     4081 caccagggcc gcgacgtgtt cgggcggcgt gaactggctg atgccatggc cgaggttgaa
     4141 gatgtgcccc ccgatacggc ccgaggtatc ccgctgcgcg ccgaagctgt cgagcgcggc
     4201 ccgcgcctcg gcccgcactg cctcgggcgg cgcgaacagc gccatcgggt cgaggttgcc
     4261 ttgcagggcc acgcggtcgc ccacgcgccg gcgggcgctg ccgagatcgg tcgtccagtc
     4321 gagcccgacg acatcggccc cggtgtcggc gatcgcctcc agccattggc cgccgccctt
     4381 ggtgaagacg atggccggga tgcgctggcc atccttctcg cgctgcagtt gcgcaagcac
     4441 gcgcctggta tacgccagcg agaacgtgcg gaaggccgcg tcggcgagca cgccgcccca
     4501 gctgtcgaac accatcacgg cctgcgcgcc ggcgtcgatc tgggcgttca ggtaggccgc
     4561 gacggcgtcg gcgttgacct ccagcagccg gtgcatgagg tcgggccgcg cgtacatgag
     4621 cgtcttgacc tggcgatagt cgtcggagcc ggcgccctcg accatgtagc aggccagcgt
     4681 ccacgggctg ccggaaaagc cgatcagcgg cacgcggccg tccagcgcct tgcggatcga
     4741 gctgacggcg tcgaagacgt agcgcagctt gtccatgtcg ggcacggcca gcttcgcgac
     4801 ggcagcctcg tcacgcacgg tcgtcgcgaa gcgcgggccc tcgccctcgg cgaacgacag
     4861 ccccagcccc atcgcgtcgg gcacggtcag gatgtcggag aacaggatgg ccgcgtcgag
     4921 cgggaagcgc tccagcggct gcagcgtcac ctcggtggcg aagtcgacgt tcgtggccag
     4981 gcccatgaaa ctgccggcgc gggcgcgcgt ggccttgtac tcgggcaggt agcgcccggc
     5041 ctggcgcatc agccagatcg gcgtgtagtc ggtcggctgc cgcaggcacg cgcgcaggaa
     5101 ggtgtcgttt ttcagggcag gccacatatg cggccgatta tccccggccc cgccgccggc
     5161 agcctgcgcg gcgtcagatg ggcttgagcg gcccgcggaa cttctccttg tgcggcttgc
     5221 cgggcagcgg cgggatctcg acctccggcg cgctggccgt cgccaccggc aggcgcgaca
     5281 ggaggttggc gatcagcttg agccggccgg ccttctggtc gttgaagtcg accagcgtcc
     5341 acggcgccca cttggtgtgc gtggcctcga gcatgcggtc gcgggcacgg ccgtactcga
     5401 cgtacttgct gcgcgcctgc aggtccaccg gcgacagctt ccactgcttg agcgggtcgt
     5461 cctggcgctc ctcgaagcgc tgctcctgct cggcctggtc gacggtgagc cagtacttga
     5521 acagccggat gccgtcgtcg accagcatcc tctcgaacac cggcgcctgc ttgaggaagg
     5581 catcggtctg cgccggggtg cagaagccca tcaccgcctc gacgcccgcg cggttgtacc
     5641 agctgcgatc gaacagcgtc atctcgccgg ccgtgggcag gtaggcgaca tagcgctgga
     5701 agtaccactc gccctgctcg cgcggcgtgg gcacgccgag cgcggcgatg tggcactggc
     5761 gcgtgttgag cttctcggag atggccttga tgacaccgcc cttgcccgcg gtgtcgcgcc
     5821 cctcgaacag cacgacgacg cgttcaccgc gctcctggag caggcgcatc acgcccgtca
     5881 gctcgacctg cagcgggcgc agctggtcct cgtaggaatg tccgtcagcc ttcttcttgc
     5941 tcatggcccc atcatgcccc ggtcgacgac ccgcggctgt cggacgggcg ctcaggctgc
     6001 acggcccggc gcggcgccgc tcgcctctac gcggccgcgc ggcaggccgc ggcgacgcac
     6061 tcggcgcagc cgcagggcac ggcgggcgcg ccggcgggcg tggcgtcgga aaagctgctg
     6121 ccgatgtcgc tggcggcgtt cgcggcggcg gcctcggcgg cgctcgtcgc gtcgccgccg
     6181 ggtgcggatg cgccgtccgc cgccgcctgc gccgccgcgg ccttcagcgc cgctgccttg
     6241 gacgccgcgt cggcgccggg cgcggccagc tgctgcagct gctgcgtggc ggcgggcgcg
     6301 gccgccgcga gcgcgtcgcc cagcgggccg cccaagggca tgtccgggat gctcatgccg
     6361 ccctccggcc ggcgtcgttg gcgccgttgc ccagcgcgcg gaacacgagc cgggagaagt
     6421 cggtcaacgc ctgcgccttg tcgccggcgg ccatggcctc gtcctggagg atctgtgcga
     6481 ccgccgggat gcggcggtcg aattcctcgc ccatcagcca ggccgtgtac acgtaggtgg
     6541 cgaccgaacg ctcgtccgcc aggccgtggc cgcgcgcgcg ctcggcctgg cgacggatcg
     6601 caccgaccag cgccggaaag tccatccggg ccgcctgctc ggggtggtgg cggcgtatga
     6661 cggccaccat gcgggcatcg aacgagtcct gccccaacct gttccactgt tcgcgcgaga
     6721 tctgcttcat cgacaccctg atcggcccgc cggcgggcat gccgcgcgat tctggcatgg
     6781 ctcgccgggg gcgaccggcg ctcggacggc accgcacggt gtatccgtga aggacatcgc
     6841 gcgcccgggc atcgggcgcc ccgttcgacg tcggcgccgc gcgggcgcgc caggcgcggg
     6901 cgctcacgtg tcacgcgcgg gcgctcgcgc ctcgcacagg tcgcgcgcga ggcgacgcgc
     6961 agcgaacgca tcgcggcccg ggctcagaac gtcggcagcg caggcaccgc cggcatcgcg
     7021 ggcatggccg gtgcggccgg catggcgggc atcgccggca tggcaggcat cgccggcatg
     7081 gcgggcatgg ccggcatcgc cggggtggcc ggcagtgcgg gcatggccgg cagcgcgggc
     7141 atggccggca aggcgggcag cgcgggctgg gcgggcatgg ccggcagcgc cggcatgtcg
     7201 ggcagcgcag gcatcgccgg catggccggc aagtcgggca ggccgttggc cgcgggcatg
     7261 gccggcatgg cgggcatcgc gggttgcgcg ggcatggccg gcatcgcggg catggcgggc
     7321 atcgccggca ggtcgggcaa ggccggcagc gcgggcaggg ccggcgtgcc gggaatcgcc
     7381 gggacggccg ggtcgaccgc cttcttgatg cagtcgccgc acgcgcacgg cacggcgggc
     7441 aggccggcgg gcgtcgtgtt agagaactcg ctgccaagat ccttggtcgc gttggccgcg
     7501 gcagcttccg cggcgccggc cgcgctgtcg gccgacgagg cggcctgcga ctgcgcgtcg
     7561 gcgagctgct gctgcgcttg cgccgcctgg gcctgcgccg cctgcgcggc cgcgtcggcc
     7621 tccccttg
//
LOCUS       DATCRO010000352         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_228550, whole genome shotgun sequence.
ACCESSION   DATCRO010000352 DATCRO010000000
VERSION     DATCRO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228550"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1483
                     /locus_tag="VIN75_09670"
     CDS             32..1483
                     /locus_tag="VIN75_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8974474.1"
                     /translation="METPGSPSAAPALRHFNDLPGPRGVPIFGNLLQIDSTRMHLQLE
                     QWCETYGPVFRLRLGPRRAVVVGDHALIAAVLRDRPEGFRRTTRFEHVWQEMGLAGGV
                     FGATGEAWKRQRRMVMAGFDPAHVKGYFPSLQRVAQRLVERWRRAARADATIDLQADL
                     MRYTVDTIAGLAFGAEVDTLGSDEDVIQRHLNRIFPALFRRLFSPLPTWRWFPSAEDK
                     ALARSVPEVAAAVDGFIAQARRRLADDPSLRAHPRNLLEAMLVAADEPSSGITDREVS
                     GNVLTMLLAGEDTTANTIAWTIHLLWTNPAALARAVEEVRRVCGDADVPTPEQVAQLD
                     YVEACAHEAMRLKPVAPQNGLEALRDTTIGDVHIPQGMIVIGVMRRDATSERHVPNAA
                     AFDPERWLGESEPAASSAKRISMPFGAGPRICPGRYLALLEMKMALAMLLAHFDLASV
                     STVDGKPPRELLQLAMAPVGLTMRLRERATAGV"
     gene            complement(1664..2659)
                     /locus_tag="VIN75_09675"
     CDS             complement(1664..2659)
                     /locus_tag="VIN75_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8974475.1"
                     /translation="MDPLSHLVTLLDPRGRMELRCLFGAAFAAPHDPVGPWRAPFHVV
                     LRGEVELLLHDSGHAISLRPGSLLVLPRGSAHTLRASAHADAAAGAQTSRASARAGAP
                     PLAPPRIRTEAGPLVDLKTNVPSADDAEIEILCGEFEFRGRRRSALLEALPECLVVEF
                     AARPEFGWLQGLLRMMAHEIEHQGAGAAAIVAELSGAVFTLAVRAHLESAADGATTLA
                     GVLGVMANPRLGPALVAMLQSPGEPWTVESLAARCHLSRAAFARLFAQHARTGPLELL
                     TALRMELASRLLAAGDQDTASIGEAVGYRSEAAFNRAFARHAGVTPGRFRRGKRR"
     gene            2762..>3127
                     /locus_tag="VIN75_09680"
     CDS             2762..>3127
                     /locus_tag="VIN75_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEY8974476.1"
                     /translation="MTTRRAPVPVSFFSIPVGLLAFANTWHVAGRLWHLSGDVAQAFT
                     LTGLAVWAALLALYVRKWFTHRAEAVAELQHPLQSAFAALVPVSSMLTSVALIPFSRG
                     AATGLFGVAVAAQLVLGLAL"
BASE COUNT          409 a         1123 c         1148 g          447 t
ORIGIN      
        1 ccggcgtggc cgagcgccgc gtcctcagtc catggagact ccaggctccc cgagcgccgc
       61 gccggcgctt cgccacttca acgacctgcc cggcccgcgc ggcgtgccga tcttcggcaa
      121 cctgctgcag atcgattcga cgcgcatgca cctgcagctc gagcagtggt gcgagaccta
      181 cgggccggtg ttcaggctgc gcctcgggcc gcgccgggcc gtggtcgtgg gcgaccatgc
      241 cctgatcgcc gccgtgttgc gcgaccggcc cgagggcttt cgccgcacga cgcgcttcga
      301 gcacgtgtgg caggagatgg gcctggccgg cggcgtgttc ggagcgaccg gcgaagcctg
      361 gaagcgccag cgtcgcatgg tgatggccgg cttcgatccc gcgcacgtga agggctactt
      421 cccgtcgctg cagcgcgtgg cgcagcgcct ggtcgagcgc tggcgccgcg cggcgcgggc
      481 cgacgccacg atcgacctgc aggccgacct gatgcgctac acggtcgaca ccatcgcggg
      541 tctcgcattc ggcgccgagg tggacacgct cggctcggac gaggacgtca tccagcgcca
      601 cctgaaccgc atcttcccgg cgctgttccg gcgcctgttc tcgccgctgc cgacgtggcg
      661 ctggttcccg tcggccgagg acaaggcgct cgcgcgcagc gtgcccgagg tcgccgcggc
      721 ggtggacggc ttcatcgcgc aggcccgccg gcgactggcg gacgatccgt cgctgcgcgc
      781 gcatccgcgc aacctgctgg aggcgatgct ggtggccgcc gacgagccca gcagcggcat
      841 caccgaccgc gaggtgtcgg gcaacgtgct gaccatgctg ctcgccggcg aggacaccac
      901 cgccaacacg atcgcctgga cgatccacct gctgtggacg aaccccgcgg cgctggcgcg
      961 cgcggtcgag gaggtgcgcc gcgtctgcgg cgatgccgac gtgccgacgc ccgagcaggt
     1021 cgcacagctc gattacgtcg aggcctgcgc gcacgaggcg atgcggctca agcccgtggc
     1081 gccgcagaac ggcctggagg ccctgcgcga cacgacgatc ggcgacgtgc acatccccca
     1141 gggcatgatc gtgatcggcg tgatgcggcg cgacgcgacc agcgagcgcc atgtccccaa
     1201 cgccgcggcc ttcgatcccg agcgctggct gggcgaatcg gaaccggcgg ccagttcggc
     1261 caagcgcatc tcgatgccct tcggcgccgg cccgcgcatc tgcccggggc gctacctggc
     1321 gctgctggag atgaagatgg cgctggccat gctgctggcg cacttcgacc tggcgtccgt
     1381 gtcgacggtc gacggcaagc cgccgcgcga gctgctgcag ctggcgatgg cgccggtcgg
     1441 cctgacgatg cgcctgcgcg agcgagcgac ggcgggcgtc tagcgtcgtg attgggaagt
     1501 tcgttgaaga atcccctcga aaacgcccgt ggccgtcgtt gccgtgctcg cgaggcattg
     1561 agcgtcgctc cgcgcgccgc ctggcccacg gacgtttgcg agaggattcg gcaacgtttc
     1621 ctctgcgctc ggagcctgtt caaggaactt cccaatctcg acactagcgt cgcttcccgc
     1681 gccgaaagcg tcccggcgtc acccccgcgt ggcgcgcgaa cgcgcggttg aacgcggcct
     1741 cggagcggta gccgacggcc tcgccgatgc tggcggtgtc ctggtcgccc gcggccagca
     1801 ggcgcgaggc gagctccatg cgcagcgcgg tcagcagttc aagcgggccg gtgcgcgcgt
     1861 gctgggcgaa caggcgggcg aaggccgcgc gcgagaggtg gcagcgggcc gcgagcgatt
     1921 ccactgtcca gggctcgccg ggcgactgca gcatcgcgac cagcgccggg ccgaggcgcg
     1981 ggttggccat cacgcccagc acgccggcga gcgtcgtcgc gccgtcggcc gcgctctcca
     2041 ggtgcgcgcg cacggccagc gtgaagacgg cgcccgacag ctcggccacg atggccgcgg
     2101 cgccggcgcc ctggtgctcg atctcgtggg ccatcatgcg cagcaggccc tgcagccagc
     2161 cgaactcggg gcgcgcggcg aactcgacga ccaggcattc gggcagcgcc tccagcagcg
     2221 cgctgcggcg gcggccgcgg aactcgaatt cgccgcacag gatctcgatc tcagcgtcgt
     2281 cggcgctggg cacgttggtc ttgaggtcga ccagcgggcc ggcctccgtg cggatgcgcg
     2341 ggggcgcgag cggcggcgcg cccgcccgcg cggacgcgcg tgacgtctgc gcgcccgcgg
     2401 ccgcatcggc gtgcgcgctc gcacgcagcg tgtgcgcgct gccgcgcggc agcaccagca
     2461 ggctgccggg ccgcagcgag atggcgtgcc cgctgtcgtg cagcagcagc tcgacctcgc
     2521 cgcgcagtac cacgtggaag ggcgcgcgcc agggcccgac cgggtcgtgc ggcgccgcga
     2581 aagccgcgcc gaacaggcag cgcagctcca tgcggccgcg tgggtcgagc agggtgacga
     2641 ggtggctcag agggtccatg cgagacgttg gagcaattcc ggtggattcg acggcattca
     2701 ggatctcgat tgtggcaagc agaatcgatt ccgtcgatca cctcctcgtt ggagtcctgc
     2761 catgacaacc cgtcgcgctc ccgtcccggt ctcgttcttc agcatccccg tggggctgct
     2821 cgcgttcgcc aacacctggc acgtggccgg gcggctgtgg catctgtccg gcgacgtcgc
     2881 ccaggcgttc acgctcaccg gcctggccgt gtgggcggcc ctgctggccc tgtacgtgcg
     2941 caagtggttc acgcaccgcg cggaggcggt cgcggagctg cagcacccgc tgcagtcggc
     3001 gttcgcggcg ctggtgcccg tctcgagcat gctgacgtcc gtcgccctga tcccgttctc
     3061 gcgcggcgcg gcgaccgggc tgttcggggt cgcagtcgcg gcgcagctcg tgctgggcct
     3121 cgcgctc
//
LOCUS       DATCRO010000353         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_228555, whole genome shotgun sequence.
ACCESSION   DATCRO010000353 DATCRO010000000
VERSION     DATCRO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228555"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VIN75_09685"
     CDS             <1..93
                     /locus_tag="VIN75_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEY8974477.1"
                     /translation="TRNLLAARSMRDLVRASGGLYRPPRRFRNW"
     gene            102..410
                     /gene="mdcC"
                     /locus_tag="VIN75_09690"
     CDS             102..410
                     /gene="mdcC"
                     /locus_tag="VIN75_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase acyl carrier protein"
                     /protein_id="HEY8974478.1"
                     /translation="MESLSFSFDGGRALAPFAPALVGVVASGNLEVLVEPSADTGCRI
                     DVDTSAHGFAPVWEAVLRDFHERHGIAGLAVAIHDMGATPAVVSLRLDQAIAAIAGGA
                     "
     gene            410..1309
                     /locus_tag="VIN75_09695"
     CDS             410..1309
                     /locus_tag="VIN75_09695"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEY8974479.1"
                     /translation="MVSSFAEMSARERLAAVVDAGSFHEWLPPSERVTSPHLAQLGVP
                     AAFDDGVAIGRAALGGRTVFIAAQEGGFMGGGVGEVHGAKLAGLLRRALRDRPDAVVI
                     LAESGGVRLHEANAGLIAVSEVMRALLDVRAAGIATVMLIGGANGCFGGMGIVARCAD
                     TVVMSDIARHAMSGPEVIESAHGAGEFDSRDRALVWRTTGGKHRWLCGDCDMLVDDDA
                     QAFRAAAIAALDAHRPMTLEELQQESALLQARADADGDNADAFELWSRLGVAEPRAVP
                     DLDADALRPRRPAAAAALARGAA"
     gene            1309..2007
                     /gene="mdcE"
                     /locus_tag="VIN75_09700"
     CDS             1309..2007
                     /gene="mdcE"
                     /locus_tag="VIN75_09700"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HEY8974480.1"
                     /translation="MDWQVVATRLFGADHAIVADGDFLCGTATFDGAPLAVVGTTNHA
                     AVGVDLALRQARAVLATVAGCPGRPLLLLVDTQGQALRRRDELLGIHRAMAHLGCCLD
                     LARRRGHPVVGLVYDQALSGGFITSGLMADACHALPQAEIRVMRVPAMARVTKLPEEL
                     LESLSRTNPVFAPGVANYVAMGGVRSLWDDDLAAGLRAALADRDTTDRRAQEGARRGG
                     RRVAADVIRRVLEA"
     gene            2007..2687
                     /gene="mdcG"
                     /locus_tag="VIN75_09705"
     CDS             2007..2687
                     /gene="mdcG"
                     /locus_tag="VIN75_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HEY8974481.1"
                     /translation="MTARRDAVRPATRLPAPLHRQQLLRLTPDGWGRVLARPRDAAER
                     ECLALWAGQNLPLVVTRQPGSGGDAIAVGLPAPARFGRLRIALSVAPGDVLALDEFPP
                     AQAVTPLLPRASVARWRALLATLALDGCRARVYGGFGWQAITRLSYVHDESDLDLLLP
                     VASAARADEVARLLATAAWDGPRLDGELLLPDGAAIAWREWLDHRQGRASRVLVKRLR
                     GVALESPA"
     gene            2684..3565
                     /gene="mdcB"
                     /locus_tag="VIN75_09710"
     CDS             2684..3565
                     /gene="mdcB"
                     /locus_tag="VIN75_09710"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEY8974482.1"
                     /translation="MSATMPLAACVAAPDADAEAFHAALGRAAIVSLYDELALAPKPG
                     LVSFADSGSHDDMDAGTFLRSLFALRHYFVRIAAQGAASADFAPLERLGIAAEARMLR
                     ATGGVNTHRGAIFTLGLLCAAAGAASHGDATTLRATLMARWGGALAARVHAARDTHGA
                     RATRAHGLRSAAEEAAQGLPTLFEHVLPALREARRAGADDRGAKLHALFTAMAEVDDT
                     NLVHRGGLQGLRDAKRLARDFLARGGGLSPDAPVRAARAHALFVERRLSPGGAADLLA
                     AALWVERVERLAGAAPR"
     gene            3562..>4383
                     /locus_tag="VIN75_09715"
     CDS             3562..>4383
                     /locus_tag="VIN75_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY8974483.1"
                     /translation="MSVALLFPGQGTQHGGMLRWLDEVDAAQPMLVRMAQVLGGDWRA
                     RLDDSAWAQSNAVAQALLTGASVAAWRALSSGLPRVVAVAGYSVGELASCVAAGMLEP
                     EDALALAGRRAAAMDACADGGGLLALSDVSAGEVAAAASRWGLEVAIRTGERRCIVGG
                     ARAALAAAAAELGARGVMARPLCVRVPSHTSALRAAVPVLAQALAGMPWRAPRVAWVA
                     GITGTVVRDPAQVRRVLAEQVATTVRWDACMDTIAERRPGAVLEVGPGTTLTRLWR"
BASE COUNT          460 a         1585 c         1743 g          595 t
ORIGIN      
        1 acgcgcaacc tgctggccgc gcgctcgatg cgcgacctgg tgcgcgcgtc gggcgggctg
       61 taccggccgc cgcgccggtt ccgcaactgg tgagcccgct catggaatcc ctgagcttct
      121 cgttcgacgg cggccgcgcg ctcgcgccgt tcgcgccggc gctggtgggc gtggtcgcgt
      181 ccggcaacct cgaggtgctg gtcgagccgt cggccgatac cggatgccgt atcgacgtcg
      241 acacctccgc gcacggcttc gcgccggtgt gggaggcggt gttgcgcgac ttccacgagc
      301 gccacgggat cgcgggcctc gcggtcgcga tccacgacat gggcgccacg ccggccgtcg
      361 tgagcctgcg gctggaccag gcgatcgccg cgatcgcggg aggcgcgtga tggtctcgag
      421 cttcgccgag atgtcggcgc gcgagcgcct ggccgcggtc gtcgacgccg gcagcttcca
      481 cgaatggctg ccgccgtccg agcgcgtgac gagcccgcac ctggcgcagc tgggcgtgcc
      541 ggcggcgttc gacgacggcg tggcgatcgg ccgcgccgcg ctgggcggtc gaacggtgtt
      601 catcgccgcg caggagggcg gcttcatggg cggcggcgtc ggcgaagtgc atggcgccaa
      661 gctggccggc ctgctgcggc gagcgctgcg cgaccggccc gacgcggtgg tgatcctggc
      721 cgagtccggc ggcgtgcggc tgcacgaggc caatgcgggg ctgatcgccg tgtccgaagt
      781 gatgcgcgcg ttgctggacg tgcgcgccgc gggcatcgcc acggtgatgc tgatcggcgg
      841 cgccaacggc tgcttcggcg gcatgggcat cgtcgcgcgc tgcgccgaca cggtcgtgat
      901 gagcgacatc gcgcgccatg cgatgtcggg gcccgaggtc atcgagtccg cgcacggcgc
      961 cggcgagttc gactcgcgtg accgcgcgct cgtgtggcgc accaccggcg gcaagcaccg
     1021 ctggctgtgc ggcgactgcg acatgctcgt cgacgacgac gcgcaggcgt tccgcgccgc
     1081 cgcgatcgcc gcgctggacg cgcatcggcc gatgacgctc gaggaactcc agcaggagtc
     1141 ggcgctgctg caggcgcgcg ccgacgcgga cggcgacaac gccgacgcct tcgagctgtg
     1201 gtcgcgcctg ggcgttgccg aaccgcgcgc ggtgcccgat ctcgatgccg acgcgttgcg
     1261 gccgcgccgt ccggccgcgg cggccgcgct cgcgcggggg gccgcgtgat ggactggcag
     1321 gtggtcgcca cgcgcctgtt cggcgccgat cacgcgatcg tcgccgacgg cgatttcctt
     1381 tgcggaacag cgaccttcga cggcgcgccg ctggccgtcg tcggcaccac caaccacgcg
     1441 gccgtcggcg tcgacctcgc gctgcgccag gcgcgcgcgg tgctcgccac ggtcgccgga
     1501 tgcccggggc ggccgctgct gctgctggtc gacacgcagg gccaggcgct gcgccggcgc
     1561 gacgagctgc tggggatcca ccgcgcgatg gcgcacctgg gctgctgcct ggacctcgcg
     1621 cggcggcgcg gtcatccggt ggtcgggctc gtctacgacc aggcgctgtc cggcggcttc
     1681 atcacctcgg ggctgatggc cgacgcatgc catgcgctgc cgcaggccga gatccgcgtg
     1741 atgcgcgtgc ccgcgatggc gcgcgtcacc aagctccccg aggaactgct ggagtcgctc
     1801 tcgcgcacga acccggtgtt cgcgccgggc gtggcgaact acgtggcgat gggcggcgtg
     1861 cgctcgctgt gggacgacga cctcgcggcc gggctgcgcg cggcgctggc cgatcgcgac
     1921 accacggatc gccgcgcgca agagggcgcc cgacgcggcg gccggcgcgt tgcggccgac
     1981 gtgatccggc gcgtgctgga ggcctgatga cggcgaggcg cgatgccgtc cggccggcca
     2041 cgcggctgcc ggcgccgctg catcgccagc agttgctgcg tttgacgccc gacggctggg
     2101 gccgcgtgct cgcgcggcct cgcgacgccg ccgagcgcga gtgcctggcg ctgtgggccg
     2161 gacagaatct gcccttggtg gtgacgcgcc agcccgggtc cggcggcgac gcgatcgcgg
     2221 tcggcctgcc ggcgccggcg cgcttcgggc gtctgcggat cgcgctgtcc gtcgcgcccg
     2281 gtgacgtgct ggcgctcgac gagttcccgc ccgcccaagc cgtcacgccg ctgctgccgc
     2341 gtgcgagcgt cgcgcggtgg cgcgcgctgc tggccacgct cgcgctcgac ggctgccgcg
     2401 cgcgggtcta cggtggcttc ggctggcagg cgatcacgcg gctgtcctac gtgcatgacg
     2461 aatcggatct cgatctgctg ctgccggtcg ccagtgccgc gcgggccgac gaagtcgcgc
     2521 gcctgctcgc gaccgcggcg tgggacgggc cgcggctgga cggcgagctg ctgttgccgg
     2581 acggcgcggc gatcgcatgg cgcgaatggc tggatcaccg gcagggacgg gcgtcgcgcg
     2641 tgctggtcaa gcggctgcgc ggcgtggcgc tggagagtcc ggcgtgagcg cgacgatgcc
     2701 cctggccgca tgcgtcgccg cgccggatgc ggacgcggag gcgttccatg cagcgctggg
     2761 ccgtgcggcc atcgtctcgc tgtacgacga gctcgcgctg gcgcccaagc cggggctggt
     2821 gtcgttcgcg gactccggca gccacgacga catggacgcc ggcaccttcc tgcgcagcct
     2881 gttcgcgctg cgccactact tcgtgcgcat cgcggcgcag ggcgcggcga gcgcagactt
     2941 cgcgccgctg gagcgcctgg gcatcgccgc ggaggcgcgg atgctgcgcg ccaccggggg
     3001 cgtcaacacg catcgcggcg cgatcttcac gctgggcctg ctgtgcgcgg ccgcgggcgc
     3061 cgcatcgcac ggcgacgcga cgacgctgcg cgcgacgctg atggcgcgct ggggcggcgc
     3121 gctcgccgcg cgcgtgcatg ccgcccgcga cacgcatggc gcccgcgcca cgcgtgcgca
     3181 cggcctgcgc agtgcggcag aagaggcggc gcagggcctg ccgaccctgt tcgagcacgt
     3241 gctgcccgcg ttgcgcgagg cgcgccgcgc gggcgccgac gatcgcggcg cgaagctgca
     3301 cgcgctgttc accgcgatgg ccgaggtcga cgacacgaac ctggtgcacc gcggtggcct
     3361 tcagggactg cgcgacgcca agcggctggc gcgcgacttc ctcgcccgcg gcggcggcct
     3421 gtcgcccgac gcgccggtgc gcgccgcgcg cgcccatgcc ctgttcgtcg agcgccgcct
     3481 gtcgcccggc ggcgccgccg acctgctcgc cgcggcgttg tgggtcgagc gcgtggagcg
     3541 cctggccggc gccgcgccgc gatgagcgtc gcgctgctgt ttcccggcca gggcacgcag
     3601 cacggcggga tgctgcgctg gctcgatgag gtcgatgcgg cgcagccgat gcttgtgcgc
     3661 atggcgcagg tgctcggcgg tgactggcgc gcacgtctgg acgacagcgc atgggcgcaa
     3721 tcgaatgcgg tcgcgcaggc gttgctcacc ggcgcgagcg tcgcggcatg gcgcgcgctg
     3781 tcgtcgggcc tgccgcgcgt ggtggccgtc gccggctaca gcgtcggcga actggcgtcg
     3841 tgcgtcgcgg cgggcatgct cgagccggag gacgcgctgg cgctggccgg acggcgcgcc
     3901 gccgcgatgg acgcgtgtgc ggacggcggc ggtctgctcg cgctgtcgga cgtgagcgcc
     3961 ggcgaggtgg ccgcggccgc ctcacgctgg ggactcgagg tcgcgattcg caccggcgaa
     4021 cgccgctgca tcgtcggcgg cgcgcgcgcc gcgctggccg cggcggcggc cgagctgggc
     4081 gcgcgcggcg tgatggcccg gcccctgtgc gtgcgcgtgc cctcacacac gagcgcgctg
     4141 cgcgcggccg tgcccgtgct cgcgcaggcg cttgcaggca tgccctggcg cgcgccgcgc
     4201 gtggcctggg tcgcagggat caccggcacc gtggtgcgcg accccgcgca agtccggcgc
     4261 gtgctcgccg agcaggtcgc cacgacggtg cgttgggacg cctgcatgga caccatcgcc
     4321 gagcgccgac ccggcgccgt gctggaagtc ggccccggca cgacgctcac gcgcctgtgg
     4381 cgg
//
LOCUS       DATCRO010000354        11321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_960364, whole genome shotgun sequence.
ACCESSION   DATCRO010000354 DATCRO010000000
VERSION     DATCRO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960364"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VIN75_09720"
     CDS             <1..865
                     /locus_tag="VIN75_09720"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY8974484.1"
                     /translation="VPVEAGVATATAAPAEPARPPAGRPHDRDRDSELPVAAMVVVDA
                     PPSVFGVGLSGRLARRPYGAAALFGWAAMRWGLMGILHHPRPAVVFLIGLGMLVAALW
                     TFRLSVLRLHDVGLSAWWVLVGAVPLVGTLAGLLLGMVPGSQGDNRFGAEPDPGSPVL
                     RMVVAVAVACIGAGFRHGLWVDDGIGSRVAASALSAPAADEAPQLPTDDDLSVFMKSP
                     KARRDFREAYWPAPKHKAFASSDSGASGWAAGAGSADAARTQALAECEKTRDAYSSEC
                     KVISVDGDWAD"
     gene            complement(876..2792)
                     /locus_tag="VIN75_09725"
     CDS             complement(876..2792)
                     /locus_tag="VIN75_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HEY8974485.1"
                     /translation="MSLIRQVWLLLSVTLVLAFAGAIGVSVHAARHYLQTQLNLKNND
                     TAQSLALTLGQQKGDPVALELAISSQFDTGFYERIRLVGPNGKTLVEKQADPHPQSAP
                     GWFVALLGLAPADGVAQVSDGWKQIGRLEVRSQSAFADDELWQSTLHTVGVLALLLAA
                     AGIFSTVGVNRIRVPLLRTVDQAKAITERRFVTVDEPDMPELRNVTRAMNAMVGRVKA
                     MFDEQAGQVELLRRQAHCDPLTGLSNRAHFLGRMKVMLDAEDGAADGALVMVRLVELQ
                     SLNRTLGRSATDRLLQDAAAAMIESSRRFSASEVGRLNGSDFALALPDVDSLRESAID
                     VGARLRSLMRVHEGGAHVVVGAVRWAHGAPVSSLLAAADQALARAESRGPWTVEADDA
                     ATGSGALGEDAWRQRIQAALAGRSAQLVDFPLVSRTGELVHLESPLRLRLGEEGELVS
                     AAQWLPMARRAQLTADVDLLAIELALQAVEGDGKPRSVNVSPSSLADSAFSTRVHALV
                     VAHADAARKLSLELAEAGAVRQLHLVRELAEQLHGSGTKIGLEHAGERLGETRGLLEA
                     GLDFVKLDASLLQGLAGDEARAQHVAGTVRMLHGIGLRVYAEGVTEVADATALWSAGV
                     DGLTGPVLQASRSA"
     gene            complement(2836..3582)
                     /locus_tag="VIN75_09730"
     CDS             complement(2836..3582)
                     /locus_tag="VIN75_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEY8974486.1"
                     /translation="MTCSRWIDADAPAPAWRAAAWLRAVLRAAAWLRAVLLRLLAVLV
                     LLAFSVQALDTDRIVRSAQKYGPTGVANAKALQQMMLALGGKDDLARLRAVNDFYNQR
                     LAYMEDIDNWGQVDYWASPLESLGKGAGDCEDYAIGKYFTLTSLGIPHARLRMVYVRA
                     SIAGAPNGFVAHMVLAYYPTPEAEPLVLDNLQPVIRPAGERPDLAPVFSFNAEGLWQG
                     VGSIRANGDPLTRLSKWREVLQRARQDGFQ"
     gene            3810..5684
                     /locus_tag="VIN75_09735"
     CDS             3810..5684
                     /locus_tag="VIN75_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8974487.1"
                     /translation="MRKTFIRFSLLALASCAAMAQSTSPVVQSVQKAIDGNPEVAAKF
                     NAFRASNDEIDVASGAWKPHLDATAAAGKRAYENTNSLPRDARFYQGGVRLELSQLLW
                     DGLGTRSEIERLGHAKLERWFEFLDATEQFGLQAATAYYDVVRYRKLVALAEDNYVQH
                     KYSFDQVQSRVNAGVGRGVDLEQVIARVALAESNLVTERANLHDVTERYIRLVGAVPP
                     AENVSAVSMVKQLPATESEAMRMAAIQSPAVAAAIENLRGARAAVAARKSPFQPRVEA
                     RAQGAAGHNLDGVLYQKHDVGAEIAVTWNIFNGGADSARERQYANLLTQAENLRDKAC
                     VDTRQTVSVAFNDVRKLGEQLGYLDRNVVSIQKARDAYRQQFDIGQRSLLDLLNSENE
                     LYTAKRSYVLAEEDLATAILRTYAGMGTLVAGLGLKRPDGQDLAPEAQNWGIDGDASS
                     RCPLEAIEVGGATFAQLDARADRMAAERAPAALRPVATPALAASAPAPAIRDVAPVPA
                     PTAAHSTTVAAQRLQDWADAWMSHNADRYLAFYAPEFKPAKGSRETWAAERRRLVSRP
                     GAIKVTLANVQTRSLTDTRVETSFDQSYSSPSLKDTMHKTLVWDRVDGEWKIVAESNR
                     "
     gene            5833..7014
                     /locus_tag="VIN75_09740"
     CDS             5833..7014
                     /locus_tag="VIN75_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974488.1"
                     /translation="MRVPRSPLTPLHQLSLAIAALTLATASAGALAQAAPAAAPAPAS
                     ASPADVAEIQRLINARQATQALKLIDDALVKNPKDPAMRFRRGVTLSLLDRNAEAIQV
                     FQKLVEDHPEMPAPYNNLAVLYGHQGDYNKARAALVAAIRTNPQYATAYQNLGDVYAQ
                     LASEAYQKALQIDSKDTVTPPKLLLLRELTNPGVAAVATSAAPAPAPAKPVQVAAATP
                     APAPAPAPAPIVAAKPAPVPPPAPKPAPVPPPAPVPAPKPSAPAPAPVTASAPAPAPA
                     VASGSAVAEVSEAVHAWASSWSRRDMAGYLAAYTPDYATAGKSHKAWEEDRKARIVPR
                     KRIAVQVSDLRVSVNGDKAQAHFKQIYESDTLSTSGHKTLDLVRSPSGKWLIKQESVG
                     G"
     gene            7089..8459
                     /locus_tag="VIN75_09745"
     CDS             7089..8459
                     /locus_tag="VIN75_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8974489.1"
                     /translation="MGSLVVAGAGLLASTELHGIPTPHATPEAALAPVGAVVALADDV
                     PPPTRAHALPVATTAATLPKDSPFAGAFKGSPESRLIGIYELIGHQQPELALDAAASL
                     THDVPGFRLAQLVYADLLSARGGAAPGFGAAGPAAAASAGLVAEVAELREEAVQRLRA
                     LQERPPEGQVPAEFIVLPKAVHHAIAVDTTRSRLYLFENGPKGMRLVSDHYVSVGKQG
                     VDKTVEGDQRTPLGVYFVSDRVGQSSLRDTFGAGAMQLNYPNLLDQLHGRTGSGIYVH
                     GVPFSTYSRPPKDSDGCVTLANDELVALMNTVPIHDTPVIITRQIHWVADDSARLNHA
                     EILDAVNRWQAVRAGDDLAALDAFYAPGAAPQTPAAPPSPPPQPTVARVHGKRVVVPP
                     PAVAPKDPVAFDNLSVMTWSDDRNKTMVVTFNERGTRTRRETTLRQYWERDAGRWKIV
                     AEGAVR"
     gene            8569..9063
                     /locus_tag="VIN75_09750"
     CDS             8569..9063
                     /locus_tag="VIN75_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974490.1"
                     /translation="MNLQKAPELLVIAATVAVVCSTFSVGAISANAATPSPAEQRYQQ
                     DRSNCMAGKTAESRKTCLTEAAAALQAARRHSLTTPTPEQIAANERKRCEAQSGDDRK
                     DCLKRAAGVDTTVSGSVAGGGDIKETVTVIPGKPGSPPVEVNPETLPPTAAGPANIPP
                     KPSN"
     gene            complement(9076..9939)
                     /locus_tag="VIN75_09755"
     CDS             complement(9076..9939)
                     /locus_tag="VIN75_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8974491.1"
                     /translation="MDSRQDPFKDVIAWIEQQLDQPLTLAQVAARAGLSPFHFSRLFT
                     ARMGRGVMAHVRGRRLVRGARRLCDEPGLQLIDLAFDCGFDSQEAFTRAFKRVFGVSP
                     GRFRSGFAVQPIEGQYPMTVPTQAAAVVRLPELVTLPGIRLAGPARRFDEATKAEIPQ
                     LWSALIGALPFAGQVRSWATYGVVSSVDPAEGCFQYMAGVGVEPDCVPPPGFATMEIR
                     AATYAVFRITLDGSALHPQVKRAMAAVWGELIPASGLTLAGGPDFELYDGRFDPLEAG
                     SVIDFHVPVQA"
     gene            complement(10329..>11321)
                     /locus_tag="VIN75_09760"
     CDS             complement(10329..>11321)
                     /locus_tag="VIN75_09760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8974492.1"
                     /translation="IIAAGSQAVRLPFFPDDPRIVDSTGALELRSTPKKMLIVGGGII
                     GLEMGTVYSTLGARLDVVEMLDGLMQGADRDLVKVWQKMNARRFDNILLKTKTVGAEA
                     TADGIKVKFEGEGGTREEMYDLVLQAVGRTPNGKKIGAERAGVIVGDRGFIPVDVQMR
                     TNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEVAAGDQHARFDARVIPSVAYTDPEVAW
                     VGLTEDQAKAQGIAVKKGLFPWSASGRAIANGRDEGQTKLLFDATTHQIVGGGIVGMN
                     AGDMIGEVALAIEMGANEVDIGRTIHPHPTLGESIGLAAEVAHGSCTDLPPQRR"
BASE COUNT         1700 a         4185 c         3909 g         1527 t
ORIGIN      
        1 ggtcccggtc gaggcaggag tcgcgacggc aacggcagcg ccggccgagc cggcccggcc
       61 gccggccggc cgcccgcacg atcgcgatcg cgattcggag ttgccggtcg cagcgatggt
      121 cgtcgtggac gcgccgccct cggtgttcgg cgtcggcctg tccggccgcc tcgcgcgccg
      181 cccgtacggc gccgcggccc tgttcggctg ggccgcgatg cgctggggac tgatgggcat
      241 cctgcaccat ccgcgtccgg ccgtcgtctt cctgatcggc ctgggcatgc tcgtcgccgc
      301 cctctggacg ttccggctga gcgtgctgcg gctgcacgat gtcgggctgt ccgcgtggtg
      361 ggtgctggtc ggcgccgtgc cgctggtcgg cacgcttgcg ggcctgctgc tcgggatggt
      421 gcccggctcg cagggcgaca accgcttcgg cgccgagccc gacccgggca gcccggtgct
      481 gcgcatggtc gtcgcggtgg cggtcgcctg catcggcgcc gggttccgcc atggcctgtg
      541 ggtcgacgac ggcatcggct cgcgcgtcgc ggcgagcgcg ctgtcggcgc cggcggccga
      601 cgaggcgcca cagttgccca ccgacgacga cctgtccgtc ttcatgaaat cgcccaaggc
      661 ccgccgcgat ttccgcgagg cgtactggcc cgcgcccaag cacaaggcgt tcgcgagctc
      721 ggacagcggc gcgtcgggct gggccgccgg cgccggcagc gcggacgcgg cacggacaca
      781 ggcgctggcc gagtgcgaga agacgcgcga cgcctacagc agcgagtgca aggtgatcag
      841 cgtcgacggc gactgggccg actgagccgg ccgggtcagg ccgagcgcga cgcctgcagc
      901 accggcccgg tcaggccgtc gacgcccgcc gaccacagcg cggtggcgtc cgccacctcg
      961 gtcacgcctt cggcgtacac gcgcaggccg atgccgtgca gcatgcgcac ggtgccggcg
     1021 acgtgctggg cacgcgcctc gtcgcccgcc aggccttgca gcaggctggc gtcgagcttg
     1081 acgaagtcca ggccggcctc gagcaggccg cgcgtctcgc ccaggcgttc gccggcgtgc
     1141 tccaggccga tcttcgtgcc cgagccgtgc agctgttcgg ccagctcgcg cacgaggtgc
     1201 agctggcgca cggcgccggc ctcggccagc tccagcgaga gcttgcgcgc ggcgtccgcg
     1261 tgggccacca cgagggcgtg cacgcgggtg gagaacgccg aatcggccag cgacgacggg
     1321 ctgacgttga ccgagcgcgg cttgccgtcg ccctcgacgg cctgcagcgc gagctcgatg
     1381 gcgagcaggt cgacgtcggc cgtcagttgc gcgcggcgcg ccatcggcag ccactgcgcg
     1441 gcggacacca gctcgccctc ctcgcccagg cgcagccgca gcggcgattc gagatgcacc
     1501 agctcgccgg tgcggctgac cagcgggaag tcgaccagtt gcgcgctgcg cccggccagc
     1561 gcggcctgga tgcgctggcg ccacgcgtcc tcgccgaggg cgccgctccc ggtggcggcg
     1621 tcgtcggcct cgaccgtcca cgggccgcgg ctttcggcgc gcgcgagcgc ctggtcggcg
     1681 gcggccagca gcgacgagac cggcgccccg tgcgcccagc gcaccgcgcc caccacgaca
     1741 tgcgccccgc cctcgtgcac gcgcatcagg ctgcgcaggc gcgcaccgac gtcgatggcc
     1801 gactcgcgca gcgaatcgac atcgggcaac gcgagcgcga agtcgctgcc gttcaggcgt
     1861 cccacctcgg aggcgctgaa gcggcgcgac gactcgatca tcgcggcggc cgcgtcctgc
     1921 aggaggcggt cggtggcaga gcggcccagc gtgcggttga gcgactgcag ctccaccagc
     1981 cgcaccatca ccagcgcgcc gtcggcggcg ccgtcctcgg catcgagcat gaccttcatg
     2041 cggcccagga agtgcgcgcg gttggacagc cccgtcagcg ggtcgcaatg ggcctggcgg
     2101 cgcagcagct cgacctggcc ggcctgctcg tcgaacatcg ccttgacgcg tcccaccatc
     2161 gcgttcatgg cgcgggtgac gttgcgcagc tcgggcatgt ccggctcgtc gacggtgacg
     2221 aagcggcgtt ccgtgatggc cttggcctgg tcgacggtgc gcagcagcgg cacgcggatg
     2281 cggttgacgc ccacggtcga gaagatgccc gcggcggcca gcagcagcgc cagcaccccg
     2341 acggtgtgca gcgtgctctg ccacagctcg tcgtcggcga aggccgactg gctgcgcacc
     2401 tcgaggcggc cgatctgctt ccagccgtcg ctgacctgcg ccaccccgtc ggccggggcc
     2461 aggcccagca gcgcgacgaa ccagcccggc gcgctctgcg gatgcgggtc ggcctgcttc
     2521 tcgacgagcg tcttgccgtt cgggccgacg agccggatgc gctcgtagaa gccggtgtcg
     2581 aactggctgg agatcgcgag ctccagggcc accgggtcgc ccttctgctg gcccagcgtc
     2641 aacgccagcg actgcgccgt gtcgttgttc ttgaggttca actgcgtctg caggtagtgg
     2701 cgcgccgcat ggacggacac gccgatcgcg ccggcgaagg ccagcaccag cgtcaccgac
     2761 aacagcaacc acacctggcg aatcagcgac atgcgctctc ctcttgggcg gcctcttcgg
     2821 gccggggcgt gctcatcatt ggaaaccatc ctgacgcgcg cgctgcagca cttcgcgcca
     2881 cttggacaac cgcgtcagcg gatccccgtt ggcacggatg ctaccaacac cctgccacag
     2941 gccttccgcg ttgaagctga acaccggcgc caggtcgggc cgctcgcccg ccgggcggat
     3001 caccggctgc aggttgtcca gcaccagcgg ctcggcctcg ggcgtcgggt agtacgcgag
     3061 caccatgtgg gcgacgaagc cgttgggcgc gccggcgatc gaggcgcgga cgtagaccat
     3121 gcgcaacctg gcgtgcggga tgcccagcga cgtcagcgtg aagtacttgc cgatcgcgta
     3181 gtcctcgcag tcgccggcgc ccttgccgag cgactcgagc gggctggccc agtagtcgac
     3241 ctggccccag ttgtcgatgt cctccatgta ggccaggcgc tggttgtaga agtcgttgac
     3301 cgcgcgcagc cgggcgaggt cgtccttgcc accgagcgcc agcatcatct gctgcagggc
     3361 cttggcgttg gcgacgcccg tcgggccgta cttctgcgcg gagcggacga tgcggtcggt
     3421 gtccagcgcc tgcaccgaga acgccagcag caccagcacg gccagcaggc gcagcagcac
     3481 cgcgcgcagc caggcggccg cgcgcagcac cgcgcgaagc caggcggccg cgcgccaggc
     3541 cggcgcgggg gcgtcggcgt cgatccagcg ggagcaagtc acgccgggcg ctgcgggaca
     3601 agggaatcgg aggtccactg gcatctatcg gccggcttcg cgcgcaatga agtggagcat
     3661 cgtcgtggtc aagccacaac ttcgagcatc aatcgacggt tgccccgaat caatgagact
     3721 tcccctcggc cacggcccgt cggccgctgc ccctgcccgg tatcattgaa acaacaacaa
     3781 acacccggtc gggacaccgc cttttccgca tgagaaagac gttcatccgc ttcagcctgc
     3841 tcgcgctggc gagctgcgcc gcgatggcgc agtccacgtc ccccgtggtc cagtcggtgc
     3901 agaaggcgat cgacggaaac ccggaagtcg ccgcgaaatt caacgctttt cgagcgtcca
     3961 acgacgagat cgacgtcgct tcgggcgcct ggaagccgca cctggacgcc acggccgccg
     4021 ccggcaagcg cgcctacgag aacaccaatt cgctgccgcg cgacgcccgc ttctaccagg
     4081 gcggcgtgcg gctggagctg agccagctgc tgtgggacgg cctgggcacg cggagcgaga
     4141 tcgagcgcct ggggcacgcg aagctggagc gctggttcga gttcctcgat gccaccgagc
     4201 agttcggcct tcaggccgcc acggcgtact acgacgtcgt gcgctaccgc aagctggtgg
     4261 cgctggccga ggacaactac gtccagcaca agtattcgtt cgaccaggtg cagtcgcgcg
     4321 tgaacgccgg cgtgggccgc ggcgtcgacc tggaacaggt gatcgcccgc gtggcgctgg
     4381 ccgagtccaa cctggtgacc gaacgggcga acctgcacga cgtcacggag cgctacatcc
     4441 gcctcgtggg tgcggtgccg ccggccgaga acgtcagcgc cgtctcgatg gtcaagcaac
     4501 tgcccgccac cgagagcgag gccatgcgca tggccgccat tcagagcccg gccgtggccg
     4561 cggccatcga gaacctgcgc ggcgcccgcg ccgccgtcgc cgcgcgcaag agcccgttcc
     4621 agccccgcgt ggaagcgcgc gcccagggcg ccgccggcca caacctggac ggcgtgctgt
     4681 accagaagca cgacgtcggc gccgagatcg ccgtcacctg gaacatcttc aacggcggcg
     4741 ccgattcggc ccgcgagcgc cagtacgcca acctgctgac ccaggccgag aacctgcgcg
     4801 acaaggcgtg cgtcgacacg cgccagaccg tctcggtcgc gttcaacgac gtgcgcaagc
     4861 tgggcgagca gctgggctac ctcgaccgca acgtcgtgtc gatccagaag gcgcgcgacg
     4921 cctatcgcca gcagttcgac atcggccagc gcagcctgct ggacctgctg aactccgaga
     4981 acgagctcta caccgccaag cgctcctacg tgctggccga ggaagacctg gccaccgcca
     5041 tcctgcgcac ctacgcgggc atgggcaccc tggtggccgg cctgggcctg aagcgccccg
     5101 acgggcagga cctggccccc gaggcgcaga actggggcat cgacggcgac gcgtcgtcgc
     5161 gctgcccgct cgaggccatc gaggtcggcg gcgccacctt cgcccagctg gacgcgcgcg
     5221 ccgacaggat ggccgccgag cgcgcgcccg cggccctgcg cccggtggcg acgccggcgc
     5281 tggccgcgtc ggcgccggcc ccggcgatcc gcgacgtggc gcccgtcccg gcgccgaccg
     5341 ccgcccactc gaccaccgtg gccgcgcagc gcctgcagga ctgggccgac gcgtggatga
     5401 gccacaacgc cgaccgctac ctcgccttct acgcgccgga attcaagccc gccaagggca
     5461 gccgcgagac ctgggccgcc gaacgccgcc gcctggtgag ccggcccggc gccatcaagg
     5521 tgacgctggc caacgtccag acccggtcgc tgaccgacac ccgggtcgag acgagcttcg
     5581 accagtccta cagttcgccg agcctcaagg acacgatgca caagaccctg gtctgggatc
     5641 gcgtcgacgg cgaatggaag atcgttgccg agagcaatcg ctgaacacgc gggctcggtg
     5701 tttcttcctg tgtccagggg cgcgaaaagc gccccttttt catgcatccg cgggcaagga
     5761 cagcccctat tttcgataga ctcacgcgca gtcgtgccgt ccctcccgcc caactcccgg
     5821 acccccgcca ccatgcgcgt tccccgatcc ccgctgacgc cgctgcacca gctctccctc
     5881 gcgatcgccg cgctcacgct ggcgacggcg tcggccgggg cgctcgcgca ggccgcgccg
     5941 gcggcggcgc ccgcgccggc ctccgcgtcg ccggccgatg tcgcggagat ccagcggctg
     6001 atcaacgcac gccaggccac ccaggcgctc aagctgatcg acgacgcgct cgtgaagaac
     6061 cccaaggatc cggcgatgcg ctttcgccgc ggcgtgacgc tgtcgctgct ggaccgcaac
     6121 gccgaggcga tccaggtgtt ccagaagctg gtcgaggacc acccggagat gcccgcgccc
     6181 tacaacaacc tcgcggtgct gtatggccac cagggcgact acaacaaggc gcgcgccgcg
     6241 ctggtggcgg ccatccgcac caatccgcaa tacgccacgg cctaccagaa cctgggcgac
     6301 gtctatgccc agctcgcgag cgaggcctac cagaaggccc tgcagatcga cagcaaggac
     6361 acggtcacgc cgcccaagct gttgctgctg cgcgagctga ccaaccccgg cgtcgccgcc
     6421 gtggccacca gcgccgcgcc tgcgccggcg cccgccaagc cggtgcaggt cgcggccgcc
     6481 acgccggcgc cggcgcctgc ccccgcgccc gcgccgatcg tcgcggccaa gcccgctccg
     6541 gtgccgccgc cggcccccaa gcccgccccg gtgccgccgc cggcgcccgt gccggcgccc
     6601 aagccgtccg cgccggctcc tgcgcccgtc accgcgtccg cgcccgcgcc ggcacccgcc
     6661 gtcgccagcg gcagcgccgt ggccgaggtc agcgaggcgg tgcacgcctg ggcgtcgtcc
     6721 tggagccgtc gcgacatggc cggctacctg gccgcctaca cgcccgacta cgccacggcc
     6781 ggcaagtcgc acaaggcgtg ggaagaggat cgcaaggcgc gcatcgtgcc gcgcaagcgc
     6841 atcgccgtgc aggtgtccga cctgcgcgtg agcgtcaacg gcgacaaggc ccaggcgcac
     6901 ttcaagcaga tctacgaatc cgacaccctc tccacgtccg gccacaagac gctcgacctg
     6961 gtgcgctcgc cctcgggcaa gtggctgatc aagcaggagt cggtcggcgg atgatgatgg
     7021 aagacgacgc cctccccgcg tcttcgaaga cgccgcgccg gccgcattgg ccgcgcacgg
     7081 cggtggcagt gggttccctc gtggtcgccg gcgccggact gctggccagc accgagctgc
     7141 acggcatccc gacgccgcat gcgacgcccg aggccgcgct cgcgcccgtc ggcgcggtcg
     7201 tcgcgcttgc ggacgacgtg ccgccgccca cgcgcgccca cgcgctgccg gtcgcgacca
     7261 cggccgccac gctgcccaag gattcgccgt tcgccggcgc cttcaagggc tcgccggaga
     7321 gccggctgat cggcatctac gagctgatcg gccaccagca gcccgagctc gcgctcgacg
     7381 cggccgcgtc cctcacgcac gacgtgcccg gcttcaggct ggcacagctc gtgtacgccg
     7441 acctgctgtc ggcacgcggc ggcgccgcgc ccggcttcgg tgccgcgggc ccggcggcgg
     7501 cggccagcgc gggcctggtc gccgaggtgg ccgaactgcg cgaggaagcc gtgcagcgcc
     7561 tgcgcgcgct gcaggagcgt ccgcccgaag gccaggtgcc ggccgaattc atcgtcctgc
     7621 ccaaggccgt gcaccacgcc atcgccgtcg acacgacacg ctcgcggctg tacctgttcg
     7681 agaacggccc caagggcatg cgcctggtca gcgaccatta cgtgtcggtc ggcaagcagg
     7741 gcgtcgacaa gacggtcgag ggcgaccagc gcacgccgct gggcgtgtac ttcgtgtcgg
     7801 accgcgtcgg gcagagttcg ctgcgcgaca ccttcggcgc cggcgcgatg cagctgaact
     7861 accccaacct cctggaccaa ttgcatgggc gcaccggctc gggcatctac gtgcacggcg
     7921 tgccgttcag cacgtactcg cgcccgccca aggactcgga cggctgcgtc acgctggcca
     7981 acgacgagct cgtggcgctg atgaacaccg tgccgatcca cgacacgccg gtcatcatca
     8041 cccggcaaat ccactgggtc gccgacgatt cggcccgcct gaaccatgcc gagatcctcg
     8101 acgcggtcaa ccgctggcag gccgtgcgcg ccggcgacga cctggccgcg ctcgacgcgt
     8161 tctacgcgcc cggcgccgcg ccgcagacgc ccgccgcgcc gccctccccg ccgccgcaac
     8221 ccacggtcgc ccgcgtgcac ggcaagcgcg tcgtggtgcc gccgccggcg gtcgcgccca
     8281 aggatccggt ggcgttcgac aacctcagcg tcatgacctg gtccgacgac cggaacaaga
     8341 ccatggtcgt caccttcaac gagcgcggca cgcgtacgcg ccgggagacg acgctgcgcc
     8401 agtactggga gcgcgacgcg ggtcgctgga agatcgtcgc ggaaggcgcc gtgcgctgaa
     8461 cggccgcgcc gctgtcgcgc gtgtttccat tgccgcgcag gatgttgcgt tctgcaggca
     8521 cgcggaatgc catgcaccgt ggagtttccc aaaagaggag tccacggcat gaacctccag
     8581 aaagcccccg aactgctcgt catcgcagcc acggtcgcgg tggtctgctc cacgttctcg
     8641 gtcggcgcga tctccgccaa cgcggccacg ccctcgccgg ccgagcagcg ctaccagcag
     8701 gatcgctcca actgcatggc cggcaagacc gccgagagcc ggaagacctg cctgaccgag
     8761 gccgccgcgg ccctgcaggc ggcgcgccgg cacagcctga cgacgcccac gcccgagcag
     8821 atcgccgcca acgagcgcaa gcgttgcgag gcgcagtccg gcgacgacag gaaggactgc
     8881 ctcaagcgtg cggccggcgt cgacaccacg gtctcgggca gcgtcgccgg cggcggcgac
     8941 atcaaggaga cggtcaccgt catccccggc aagcccggca gcccgccggt cgaggtcaac
     9001 cccgagacgc tgccgccgac ggcggccggc ccggccaaca tcccgcccaa gcccagcaac
     9061 tgaagccgcc ggggatcagg cctgcacggg cacgtggaaa tcgatcaccg agccggcctc
     9121 gagcgggtcg aagcgtccgt cgtagagctc gaagtcaggg ccaccggcca gcgtcaggcc
     9181 ggaggccggg atcagttcgc cccagaccgc ggccatcgcc cgcttgacct gcgggtgcag
     9241 cgccgagcca tccagcgtga tgcggaagac ggcataggtc gcggcccgga tctccatcgt
     9301 cgcgaaaccc ggcggcggca cgcagtccgg ctcgacgccg acgccagcca tgtactggaa
     9361 gcagccttct gccggatcga cgctcgagac gacgccatag gtcgcccagc tcctgacctg
     9421 cccggcgaac ggcagcgcgc cgatgagcgc ggaccacagc tgcgggatct cggccttggt
     9481 cgcctcgtcg aagcggcgcg ccgggccggc gaggcggatg ccgggcaacg tcacgagttc
     9541 ggggaggcgg acgacggcgg cggcctgggt gggtacggtc atggggtatt gcccttcgat
     9601 gggttggacc gcgaagccgc tgcggaaccg ccccggcgag acgccgaaca cgcgcttgaa
     9661 agcgcgcgtg aacgcctcct gggagtcgaa gccgcagtcg aacgcgaggt cgatcagttg
     9721 caggccgggc tcgtcgcaga ggcgacgcgc cccgcgcacg agccggcgcc cgcgcacgtg
     9781 ggccatcacg ccgcggccca tgcgcgcggt gaacagccgc gagaagtgga acggcgacag
     9841 gccggcccgc gccgcgacct gcgccagcgt caacggctgg tcgagctgct gctcgatcca
     9901 ggcgatgacg tccttgaacg ggtcttggcg gctgtccatg ggcggcatca tcgcggagcg
     9961 gcgggcctcc tgtcctgacc gttcctgctg ccggggggtc gccgtcgggg gtcaggtctt
    10021 gaatgttgcg gggtcaggtc ttgaatcatg cactccaccg caggtctggc acgggccgtc
    10081 gtgtctgctg cggtgtgagc cggccgcagt gtcgagtctt gtccttttgc gtcatcacca
    10141 tccctgctta gaaaaggcaa gcccctgaca ccaatgcgga cacgaaagtg ctcatttcaa
    10201 gacctgacac tggcgagaaa gtgcgcgatt caagacctga ccccgcaaca ttcaagacct
    10261 gaccccgtcg ttgaatccgg aaagcagaac aggcgccgca gcgcctcctc atcgggtcga
    10321 agcaacgatc acctgcgctg cggcggcagg tccgtgcagc tgccatgggc cacttccgcc
    10381 gccaggccga tcgattcgcc cagcgtcggg tgcgggtgga tcgtgcggcc gatgtcgacc
    10441 tcgttggcgc ccatctcgat ggcgagcgcg acctcgccga tcatgtcgcc ggcattcatg
    10501 ccgacgatgc cgccgcccac gatctggtgc gtcgtcgcgt cgaacagcag cttggtctgc
    10561 ccttcgtcgc ggccgttggc gatggcgcgg ccggaagccg accacgggaa caggcccttc
    10621 ttgaccgcga tgccctgcgc cttggcctgg tcctcggtca ggcccaccca ggccacttcc
    10681 ggatcggtgt aggccacgct ggggatcacg cgggcgtcga agcgggcgtg ctggtcgccg
    10741 gcggcgactt ccgcggccac gtggccctcg tgcaccgcct tgtgcgccag catcggctgg
    10801 ccgacgatgt cgccgatcgc gaagatgtgc ggcacgttgg tgcgcatctg cacgtccacc
    10861 gggatgaagc cgcggtcgcc gacgatgacg cccgcgcgtt ccgcgccgat cttcttgcca
    10921 ttgggcgtgc ggccgacggc ctgcagcacg aggtcgtaca tctcctcgcg cgtgccgccc
    10981 tcgccttcga acttcacctt gatgccgtcg gccgtcgcct cggcgcccac cgtcttggtc
    11041 ttgagcagga tgttgtcgaa gcgccgggcg ttcatcttct gccagacctt gacgaggtcg
    11101 cggtcggcgc cctgcatcag gccgtccagc atctcgacga cgtccaagcg cgcgcccagc
    11161 gtcgagtaca cggtgcccat ctccaggccg atgatgccgc cgccgacgat cagcatcttc
    11221 ttcggcgtgg agcgcagttc cagcgcgccg gtggagtcga cgatgcgcgg gtcgtccggg
    11281 aagaacggca ggcgcacggc ctgcgagccg gccgcgatga t
//
LOCUS       DATCRO010000355         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_465205, whole genome shotgun sequence.
ACCESSION   DATCRO010000355 DATCRO010000000
VERSION     DATCRO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465205"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..2260)
                     /gene="ptsP"
                     /locus_tag="VIN75_09765"
     CDS             complement(467..2260)
                     /gene="ptsP"
                     /locus_tag="VIN75_09765"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8974493.1"
                     /translation="MSIQVFGLPVSRGVAIGRAVLVASSRVDVAHYYVDAGMIEAEVR
                     RLRRARDMVVDELTALKGDVPEDAHDELVALLDVHLMLLQDDSLSEATRQWIVERHYN
                     AEWALSAQLEVLARQFDEMEDEYLRERKGDLEQVVERVIAALGRLGGGDGDGEADSDI
                     PAHGQRDFAGEDPLILVANDIAPADMLKFRRSVFTGFVTDVGGRNSHTAIVARSMDIP
                     AVVGAREASRIVTQDDWIIIDGDAGVVIVNPSPIVLEEYRFRQRQVELERARLDRLRH
                     TPAVTLDGNPVELLANIELPSDAQAVLDAGAVGVGLFRSEFLFMNRAGRLPGEEEQYE
                     AYSKVVKALSGLPVTIRTVDIGADKQLDRAPQQMERNDHNLNPALGLRAIRWSLSEPE
                     MFGEQLRAILRASVHGKVRLLVPMVAQLREVRLLLEAIERAKAQLRARAQPYLDVAIG
                     AMIEVPAAALVLPSLLKYFDFVSIGTNDLIQYTLAIDRADEAVSHLYDPWHPAVLRLI
                     SQTIATANAQGKDVCVCGEMAGDPAFTELLLGMGLRSFSMHPTQVSSIKKRILRVDSS
                     LWSRHVDLVCEAEDPQAIYARLQTAVPERRG"
     gene            2457..3278
                     /locus_tag="VIN75_09770"
     CDS             2457..3278
                     /locus_tag="VIN75_09770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY8974494.1"
                     /translation="MTDFVTTPIAVDSPRRADRAALRAERAPAAPTLQVVWARHLDDV
                     RAAQRLRHAVFVEEMGARPTPLAGAPAGHDVDLFDAFCEHLIVRTAPAGDEPGEVVGT
                     YRVLTPSAARRAGSFYSDTEFDLTRLRPLRDRMVELGRSCVHPDHRTGGVILTLWSAL
                     SQFMEQNAMETVIGCASISMRDGGHCAASLWARLRETHLAPADRQVRPRVALPVDDLD
                     QTLDVEPPALIKGYLRMGAKVLGAPAWDPEFNTADLPMLTRVADIPARYQRHFAA"
     gene            complement(3304..3573)
                     /locus_tag="VIN75_09775"
     CDS             complement(3304..3573)
                     /locus_tag="VIN75_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8974495.1"
                     /translation="MIRTPLVISNKLGLHARASAKLTKLASSFKCEVFMSRNGRRVNA
                     KSIMGIMMLAAGLGSDVELETDGEDEREAAEQIAALVNDKFGEGQ"
     gene            complement(3542..3991)
                     /locus_tag="VIN75_09780"
     CDS             complement(3542..3991)
                     /locus_tag="VIN75_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEY8974496.1"
                     /translation="MAAVLLIAHAPLASALLSVAGHCYEEKGHLFEAIDVEPAMSPET
                     VESLARAALARLNAPEVLVLTDVFGATPCNVAQRLADGVNVRVVAGVNVPMLWRSLCY
                     LGEPLDALVARAVSGASQGVMQVAQTRPQNQVQPPLSNDPNSSRHQQ"
     gene            complement(4149..4673)
                     /locus_tag="VIN75_09785"
     CDS             complement(4149..4673)
                     /locus_tag="VIN75_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974497.1"
                     /translation="MLTSLAVLAACSPTFDWRSIPLPDTPLVAELPCRPARFQRDVTV
                     AGVPLQLFMLSCEAGGVTYGVATADVVDPARVDAVLFALRASAAGAIRSADSTAGALN
                     LDGITPFPGNTSAHLHGRRPDGGTIEEAVRVFARGTRVYQASAVGTSLPDAALRPFED
                     GLRFDLEKPVAGPS"
     gene            4967..>5255
                     /locus_tag="VIN75_09790"
     CDS             4967..>5255
                     /locus_tag="VIN75_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974498.1"
                     /translation="MTDQASDRPPTQPDEGHWREIIGQIGTEVGLPLSAALERVTVLA
                     TTGKIDRAGLRMLREEIERARRAGMIGQQLARFASGRIRLSPEQVSVTQMLR"
BASE COUNT          825 a         1849 c         1767 g          814 t
ORIGIN      
        1 gcgtcgcgcc aacctgttgt cagcgcagaa gcggaattct aagttgcttt tcaaaacctt
       61 gtcaagcact cagttttcac ttcgttttcg ccgaccgcgt tatctgctca aactcgctgc
      121 gccgtagcgc tgcaatttca aactgaatct cgatcagcga agcctgccat tctatgctcg
      181 attccgaatc gtcgtcaagc aaaaatttca gactcttcgc tggccgactc ggcctgcaga
      241 ggcgttgtct gtagcgagcc ccaagaccca gccagtcacc gcatctgcgc tgacttgcga
      301 tcagcgaagc ctgccattct atgtcgggtc ttttgaaact tgcaagcact ttgcagaaat
      361 cttccggacg gcccgatctg cgcctcgatc accgcccggc ttgccagcgg gaatgcgaaa
      421 gggcgccgac acgcggcgcc ctccatggcg aacgcagacg tcggcgtcag ccccgccgct
      481 cgggcaccgc cgtctgcagc cgcgcgtaga tcgcctgcgg atcctcggcc tcgcacacca
      541 ggtcgacgtg cctgctccag agggacgagt ccacgcgcag gatgcgcttc ttgatggacg
      601 acacctgcgt cggatgcatc gagaagctgc gcaggcccat gcccagcagc agctcggtga
      661 acgccgggtc gccggccatc tcgccgcaga cgcagacatc cttgccctgc gcgttggccg
      721 tggcgatggt ctgcgagatc agccgcagca ccgcgggatg ccacgggtca tagagatggg
      781 acaccgcctc gtcggcgcgg tcgatggcca gcgtgtactg gatcaggtcg ttggtgccga
      841 tcgagacgaa gtcgaagtac ttgagcagcg acggcagcac cagcgcagcg gcgggcacct
      901 cgatcatggc gccgatggcg acatccagat agggttgcgc ccgcgcgcgc aactgcgcct
      961 tggcgcgctc gatggcctcc agcagcaggc gcacctcgcg cagctgcgcc accatgggca
     1021 ccagcagccg aaccttgccg tgcacgctcg cgcgcaggat ggcgcgcagc tgctcgccga
     1081 acatctccgg ctcggacagg ctccagcgga tcgcccgcag tcccagcgcc gggttcaggt
     1141 tgtggtcgtt gcgctccatc tgctggggcg cgcggtcaag ctgcttgtcg gcgccgatgt
     1201 cgaccgtgcg gatcgtgacc ggcagccccg acagcgcctt gacgaccttg ctgtaggcct
     1261 cgtactgctc ttcctcgccg ggcaggcggc cggcgcggtt catgaacagg aactcgctgc
     1321 ggaaaaggcc gacacccacg gcgccggcgt cgagcacggc ctgtgcgtcg ctcggcagct
     1381 cgatgttggc cagcagctcg accgggttgc cgtccaacgt cactgccggc gtgtggcgca
     1441 ggcgatccag gcgagcgcgt tccagctcga cctggcgttg gcggaagcgg tattcctcga
     1501 gcacgatggg agaaggattg acgatcacca cgccggcgtc gccgtcgatg atgatccagt
     1561 cgtcctgcgt gacgatgcgg ctcgcctcgc gggcgccgac caccgccgga atgtccatgc
     1621 tgcgcgcgac gatggccgtg tgcgaattgc ggccgccgac gtcggtgacg aaccccgtga
     1681 acacgctgcg cctgaatttg agcatgtcgg cgggcgcgat gtcgttggcc accaggatca
     1741 gcgggtcttc gccggcgaag tcgcgctggc catgggccgg gatgtcgctg tcggcttcgc
     1801 cgtcgccgtc gccgccgccg agccgcccca gcgcggcgat gacgcgttcg accacctgct
     1861 ccaggtcgcc tttgcgctcg cgcaggtact cgtcctccat ctcgtcgaac tggcgggcca
     1921 gcacttcgag ctgcgccgac agcgcccact cggcgttgta gtggcgctcg acgatccatt
     1981 gccgcgtggc ctcggacagc gagtcgtcct gcagcagcat caggtggacg tccagcagcg
     2041 cgacgagctc gtcgtgcgcg tcctccggca cgtcgccctt gagcgccgtg agctcgtcga
     2101 ccaccatgtc gcgcgcccgg cgcagccggc gcacctcggc ctcgatcatg cctgcgtcga
     2161 cgtagtagtg cgcgacgtcg acgcggctgg atgccaccag caccgcacgg ccgatggcga
     2221 cgccacgcga caccggcaga ccgaacacct gaatgctcac gaaactcctc cgcgaaccgc
     2281 acgccggatg cgcgtcgggc gccggcgggt tcgtccggtc aatgtagcag caagccggcg
     2341 cgccgccctg cgccaaaccg gggattccgc cacggtgtca cgccaggttc atcgccgctt
     2401 cacgggcgcg tcatcgcggc ccgggagcat gcctgccatc cttggggagc attgcgatga
     2461 ccgatttcgt cactacgccc atcgcggtcg acagcccgcg ccgcgccgac cgggccgccc
     2521 tgcgcgccga gcgcgcgccg gcagccccga cgctgcaggt cgtgtgggcc cgccacctgg
     2581 acgacgtccg cgcggcccag cgcctgcgcc acgctgtctt cgtcgaggag atgggcgccc
     2641 gtccgacgcc gctggccggc gcgcccgcgg ggcacgacgt cgacctgttc gacgcgttct
     2701 gcgagcacct gatcgtgcgc accgcgcccg ccggcgacga acccggcgag gtcgtgggca
     2761 cctaccgcgt gctcacgccc agcgccgcgc gccgcgccgg cagcttctac agcgacaccg
     2821 aattcgacct cacccgactg cgcccgctgc gcgatcgcat ggtggagctg ggccgcagct
     2881 gcgtgcaccc ggatcaccgc accgggggcg tgatcctgac gctgtggagc gcgctgtcgc
     2941 agttcatgga acagaacgcg atggagacgg tgatcgggtg cgccagcatc tcgatgcgcg
     3001 acggcggcca ctgcgccgcc agcctgtggg ccaggctgcg cgagacgcac ctcgcgccgg
     3061 ccgaccgcca ggtgcgcccc cgcgtggcgt tgccggtgga cgacctggac cagacgctgg
     3121 acgtcgagcc gcccgcgctg atcaagggct acctgcgcat gggcgccaag gtgctgggcg
     3181 cgccggcgtg ggatccggaa ttcaacacgg ccgacctgcc gatgctgacc cgcgtggccg
     3241 acatcccggc ccgctaccag cgccacttcg cggcctgaag cgccgcgcgc ggcgatccgc
     3301 cgcctactgg ccttcgccga acttgtcgtt gacgagcgcg gcgatctgct cggccgcctc
     3361 ccgctcgtcc tcgccgtcgg tctcgagctc cacgtcggag ccgaggccgg cggccagcat
     3421 catgatgccc atgatgctct tggcgttgac ccgccggccg ttgcgcgaca tgaagacctc
     3481 gcacttgaaa ctgctggcca gcttggtgag cttggccgag gcccgggcat gcaagccgag
     3541 cttattgctg atgacgagag gagttcggat cattcgagag cgggggttga acctggttct
     3601 gtggacgcgt ctgcgccacc tgcatgacgc cttgcgaggc gcccgagacg gcacgggcga
     3661 ccagcgcgtc gagcggctcg cccagatagc acagcgagcg ccacagcatc ggcacgttga
     3721 cgccggccac cacgcgcacg ttgacgccat cggccagacg ctgtgccacg ttgcagggcg
     3781 tggcgccgaa cacgtcggtc agcaccagca cctcgggcgc gttcaggcgc gcgagcgccg
     3841 cgcgggcgag cgactccacc gtctcgggcg acatcgccgg ctcgacgtcg atcgcctcga
     3901 acaggtggcc cttctcctcg tagcaatgcc cggcgacgga aagcagcgcc gaggcgaggg
     3961 gagcatgagc gatcagcagg acagcggcca cgtggattca gacccgagcg ccatgacgcg
     4021 gcgcaaacaa tcgattatcg gcctggaagc gcggcaaacc taggccgcgg actctgggtg
     4081 cgggcgcgcg gtcggccatt cgtggcggtt ttgcacgctt gcgacggcgc gacgcccccg
     4141 gctggaccct agctggggcc ggcgacgggc ttttccaggt cgaagcgaag gccgtcctcg
     4201 aacgggcgca gcgccgcgtc gggcaggctg gtgccgacgg cgctcgcctg gtagacgcgc
     4261 gtcccgcggg cgaagacccg gacggcttcc tcgatcgtcc cgccgtcggg ccggcggcca
     4321 tgcaggtgcg cactggtgtt tcctggaaac ggggtgatcc cgtccaggtt caacgcgcca
     4381 gccgtcgaat cggccgaccg gatggcgccg gccgcgctgg cacgcagcgc gaacagcacc
     4441 gcgtccaccc tggccgggtc gaccacgtcg gccgtggcga cgccgtaggt gacgccgccc
     4501 gcctcgcacg acagcatgaa cagctgcaat ggcaccccgg cgacagtcac gtcgcgctgg
     4561 aagcgggcag ggcggcaggg cagttccgcg acgagcgggg tgtcgggcaa cgggatgctg
     4621 cgccagtcga acgtcggcga acaggccgcc agcacggcca gcgacgtcag caccagtcgc
     4681 tgctcgagcg tgggtctccg gcaactgtca acgggtgtat ccgaatgttt ttgagcgggc
     4741 atcggttcat catgtgcagg aatgcgcggc cgactgagcc gttttcaccc tcctggggga
     4801 tggaatcacc ctctggcggt gggcaatatg cgggtcgtca ggatgcagtt cgctacaatt
     4861 tgtaactagc gcaactccag ttgcgcggcg cacatccggt gccgcccgct tcgcgcgccg
     4921 gagggaatga ataatggcaa tgactctggc ttcgagccgc tccatcatga ccgatcaggc
     4981 ttccgacagg cccccgacgc agccggacga gggacattgg cgcgagatca tcggccagat
     5041 cggcaccgag gtcggactgc cgctgtcggc cgcgctcgag cgcgtcaccg tgctcgccac
     5101 caccggcaag atcgaccgcg ccgggctgcg catgctgcgc gaggagatcg aacgcgcccg
     5161 ccgcgccggc atgatcggcc agcagctggc ccgcttcgcg tccggccgca tccgcctgtc
     5221 gcccgagcag gtcagcgtca cccagatgct gcgcg
//
LOCUS       DATCRO010000356         5561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1016142, whole genome shotgun sequence.
ACCESSION   DATCRO010000356 DATCRO010000000
VERSION     DATCRO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016142"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VIN75_09795"
     CDS             complement(<1..105)
                     /locus_tag="VIN75_09795"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8974499.1"
                     /translation="MNVLVLHGPNLNLLGTREPAVYGSTTLPDIDRELQ"
     gene            complement(318..890)
                     /locus_tag="VIN75_09800"
     CDS             complement(318..890)
                     /locus_tag="VIN75_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8974500.1"
                     /translation="MTGLTRRDVVGAVVLGGAALGLGAWLRHRQQAPVVVGDAAAAAA
                     SAAGLALPPLTPIPSPLKTMDGRTLSDADIGGRFVLLNFWAPWCPPCVREMPEIDRFA
                     RSQAGRNVLVIGLAIDEQPAVEKFVAAHPVSFPISILGYAGLAWARRLSNDPNVALPF
                     TAVFDRSQRLAQHKFGATNAAELTEWVRIS"
     gene            complement(887..1489)
                     /locus_tag="VIN75_09805"
     CDS             complement(887..1489)
                     /locus_tag="VIN75_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974501.1"
                     /translation="MELDREQQEVAQTAARMIVEDGLDYATARRKALQEHGDGRRLRD
                     APSNEAIEDEVRAHIAIFHADTQPAELRALREAALRWMLRLAEFRPHVGGAVWRGTAT
                     RQSIVRIDLYCEDTKAPELAFLNRGVDYETISEPLGRGEHSLVLVVDERNADLGDWVT
                     IHFNVLDLDDLRGALRPDARGQTWRGDAAALQRLLEADAS"
     gene            complement(1596..2348)
                     /locus_tag="VIN75_09810"
     CDS             complement(1596..2348)
                     /locus_tag="VIN75_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8974502.1"
                     /translation="MRNVGRLVGMLLVATLALQLFFLLRIALMTVVAPESTTFQRSEI
                     WRIVLAKHQLPWLQDGVPGSRISDNLRRAVIASEDAGFVEHHGVEWDAVEKAWNRNEK
                     AQARAEAQQSRATRRGRDGKPREVVAKVVGGSTISQQLAKNLFLSGERNILRKGQELV
                     ITFMLEFVLGKERILTTYLNNVEWGEGVFGAQAASEHYFRTNAATLGAAQAARLAVML
                     PAPKRFEKAPSSPYVLGRSATIVARMGGVDLP"
     gene            complement(2357..3217)
                     /gene="aroE"
                     /locus_tag="VIN75_09815"
     CDS             complement(2357..3217)
                     /gene="aroE"
                     /locus_tag="VIN75_09815"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8974503.1"
                     /translation="MTRLAVVGNPVAHSRSPQIHARFAAATGLAVDYTRLEAPLDAFV
                     ATLREFAGSGARGCNVTVPFKGEAFAACTRLSERARRAEAVNTIVFEADGWFGDNTDG
                     AGLVRDIELNAGVALQGKRVLVLGAGGAAAGALDPLLAARPAALVVANRTPAKAQAMI
                     ERFAAGAARLDVPLSATPLEAPGAGFDVLVNATAASLQAAVPPLPAGTLKPGALALDM
                     MYGAATVPFLRWARAQGAVARDGLGMLVEQASEAFFVWMGRRPATFQVLADLRREVDA
                     QIEAASKASA"
     gene            complement(3332..4222)
                     /locus_tag="VIN75_09820"
     CDS             complement(3332..4222)
                     /locus_tag="VIN75_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8974504.1"
                     /translation="MFAAEPLSVRIRKWRPSVMQLALTISIALHAAVLGVRIVDPQDF
                     NRVFQDTPLEVVLVNARSNEAPTKAQAIAQANLAGGGEAEAGLATSPLPPSPNELVGD
                     DQTEASRKIQTDEQEQQQLLATVRRDIAAMPVPDPRKATMTQAERETEELRQQKLRLL
                     AAIEKRINEENSRPRRRYISPSTREEVYAIYYDKLRRKIEDKGTANFPSLNGHKLYGE
                     LTVNLTIDARGRIVESEIVHSSNSRFLDQRALAIAQAASPFGGFSDDMKRKADQIVVT
                     SRFRFTKTEGMQATMTTNGG"
     gene            complement(4281..>5561)
                     /locus_tag="VIN75_09825"
     CDS             complement(4281..>5561)
                     /locus_tag="VIN75_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY8974505.1"
                     /translation="LSVRGLGTGSVVFGVHIAAPGLAILPDSPVDKVARDRLSTVYMP
                     GWKITMLPDAIVEKYTLAEGRDCPALSLYVTFDEATLEIRGSETRVERVPIVANLRHD
                     KLDADVTEASLLGEAPADYAFATELAFTFRLARHLKALREVVRGKPENFNRPDYNFRL
                     QAAEGRSPASEPAGDETVTISERRRGAPLDLIVSEAMILANSTWGGWIAEMGVPGIYR
                     SQASLAPGVKVRMGTKPAPHAGMGVAQYTWATSPLRRYVDLVNQWQIIACVRNGRMAP
                     LSAPFKPKDATLFSVISGFDTAYSAYNGFQTGIERFWTLRWLQQNDVAELDATVLKEG
                     LVRADTLPLVFKAIGCDGLARGTHVRVRVDGVDLLTLDVHARLVARLDDESAAAEAAA
                     DEPDDAEEAGAGPLTLAIDVTEETPPAEGAATPA"
BASE COUNT          739 a         1917 c         2053 g          852 t
ORIGIN      
        1 ctgcaattcg cgatcaatgt ccggcaacgt ggtacttcca tacacagccg gctcgcgtgt
       61 accgagcagg ttgaggttgg gaccgtgaag cacgaggacg ttcatgcgag gcgcgatgcg
      121 gcggcggagg ccggcgtgtc aattcgaaag aggcggactt tacgctttgt tgagcgccgt
      181 tcccgccact aagcgagaat ttaccctaaa aagagtgctt gacaactcta tccgcgttgg
      241 gcaatgcggt tgagcgcggt tccgcgcgtt ccggggcctc gttcggagca accacgcgcc
      301 ggttgctgcg agttccttca ggaaatgcgc acccactcgg tcagttcggc ggcattggtc
      361 gctccgaact tgtgctgcgc gagtcgctgc gatcggtcga acaccgccgt gaagggcagg
      421 gcgacgttcg gatcattgct gaggcggcgg gcccaggcaa gcccggcata cccgaggatg
      481 gagatcggga atgagaccgg atgggcggcc acgaatttct cgacggcggg ctgttcgtcg
      541 atcgccaggc cgatcacgag cacgttcctg ccggcctgcg atcgcgcgaa gcggtcgatc
      601 tcgggcatct cccggacgca cggcgggcac cacggcgccc agaagttgag cagcacgaag
      661 cgcccgccga tgtcggcgtc gctcagcgtg cgcccgtcca tggtcttgag cggcgacggg
      721 atcggcgtga gcggcggcag ggcgagaccg gccgccgagg ccgcggcggc ggcggcgtcg
      781 ccgacgacca ccggcgcctg ctgccgatga cgcagccagg cgcccagccc gagcgcggcg
      841 ccgcccagga cgacggcgcc gacgacgtcg cgtcgcgtca ggccggtcat gacgcgtccg
      901 cctcgagcag ccgctgcagg gccgccgcgt cgccgcgcca ggtctggccg cgcgcgtcgg
      961 gcctgagggc gccgcgcagg tcgtccaggt cgaggacgtt gaagtggatc gtcacccagt
     1021 cgccgaggtc ggcgttgcgc tcgtcgacca ccaggaccag cgaatgctcg ccgcggccca
     1081 gcggctcgct gatggtctcg taatcgacgc cgcggttcag gaacgccagt tcgggcgcct
     1141 tggtgtcctc gcagtagagg tcgatgcgca cgatggactg gcgcgtggcc gtgccgcgcc
     1201 agacggcgcc gcccacgtgc ggccggaact cggccagccg cagcatccag cgcagcgcgg
     1261 cctcgcgcag cgcgcgcagt tcggcgggtt gcgtgtcggc atggaagatc gcgatgtggg
     1321 cgcggacttc gtcctcgatc gcctcgttgc tgggcgcgtc acgcaggcgg cggccgtcgc
     1381 cgtgctcctg caacgccttg cggcgggcgg tggcgtagtc caggccgtcc tcgacgatca
     1441 tgcgcgcggc ggtctgcgcg acttcctgct gttctctgtc cagttccatg tcggttcgcg
     1501 ggccctcgag gggcggttca acacgccatc atggcagctc cgggacgccc ctgcggacga
     1561 cgatggcggc cggctcgacc ggcgtcaggc cggcttcagg gcagatcgac gccgcccatg
     1621 cgcgccacga tcgtcgccga gcggcccagc acgtagggcg acgacggcgc cttctcgaag
     1681 cgcttgggcg caggcagcat cacggccagc cgggcggcct gggccgcgcc gagcgtggcg
     1741 gcgttcgtgc ggaaatagtg ttccgacgcc gcctgggcgc cgaacacgcc ctcgccccat
     1801 tcgacgttgt tcaggtaggt ggtgaggatg cgctccttgc ccagcacgaa ctccagcatg
     1861 aaggtgatga ccagctcctg gcccttgcgc aggatgtttc gctcgccgga caggaacagg
     1921 ttcttggcga gctgctgcga gatcgtcgag ccacccacca ccttggccac cacctcgcgc
     1981 ggcttgccgt cgcggccgcg ccgcgtggcc cggctctgct gcgcctcggc gcgcgcctgc
     2041 gccttctcgt tcctgttcca ggccttctcc acggcgtccc actcgacgcc gtggtgctcg
     2101 acgaagccgg cgtcctcgct cgcgatcacg gcgcggcgca ggttgtccga gatgcggctg
     2161 cccggcacgc cgtcctggag ccacggcagc tggtgcttcg cgagcacgat gcgccagatc
     2221 tcgctgcgct ggaacgtcgt cgattccggg gcgaccaccg tcatcagcgc gatgcgcagc
     2281 aggaagaaca gctgcagcgc gagcgtggcg accagcagca tgccgaccag gcggccgacg
     2341 ttgcgcacgc ccgagttcac gccgaggcct tcgaggcggc ctcgatctgc gcgtcgacct
     2401 cgcgccgcag gtcggccagc acctggaatg tggcgggacg tcggcccatc cagacgaaga
     2461 aggcctcgga cgcctgctcc acgagcatgc ccaggccgtc gcgcgcgacc gcgccctggg
     2521 cgcgcgccca gcgcaggaag ggcacggtgg cggcgccgta catcatgtcc agcgccagcg
     2581 cgccgggctt gagcgtgccg gccggcaaag gcgggacggc ggcctgcagg ctcgcggcgg
     2641 tggcgttgac cagcacgtcg aagccggcgc cgggcgcctc cagcggcgtg gccgacagcg
     2701 gcacgtccag ccgcgcggcg ccggcggcga agcgctcgat catggcctgc gccttggccg
     2761 gcgtgcggtt ggcgacgacc agcgccgccg gccgcgcggc cagcagcggg tcgagcgcgc
     2821 cggccgccgc gccgccggcg cccagcacga gcacgcgctt gccttgcagc gccacgccgg
     2881 cattgagctc gatgtcgcgc accaggccgg cgccgtcggt gttgtcgccg aaccagccgt
     2941 cggcctcgaa cacgatcgtg ttgacggcct cggcccggcg cgcacgctcg gacagccgcg
     3001 tgcaggcagc gaacgcctcg cccttgaacg gcacggtgac gttgcagccg cgcgcgccgg
     3061 acccggcgaa ctcgcgcagc gtggcgacga aggcgtccag cggcgcctcg aggcgcgtgt
     3121 agtcgacggc caggccggtg gccgcggcga agcgcgcgtg gatctgcggc gagcggctgt
     3181 gggcgacggg attgccgacg acggcgaggc gcgtcattgg agcccctccc gcgccgggta
     3241 cggcgctccg tcccccgagg ggactgcggg ccggcttggg gcggcccggc gctcggcccg
     3301 ctggcggctc gatcgaggat gtgccatcag gtcaacctcc gttggtggtc atcgtggcct
     3361 gcatgccctc ggtcttcgtg aaacggaagc gcgaggtgac gacgatctgg tcggccttgc
     3421 gcttcatgtc gtcgctgaaa ccgccgaagg gcgacgcggc ctgcgcgatc gccagcgcgc
     3481 gctggtcgag gaagcgcgag ttggagctgt gcacgatctc ggactcgacg atgcggccgc
     3541 gcgcgtcgat cgtgaggttg accgtcagct cgccgtacag cttgtggccg ttcagggacg
     3601 ggaagttggc ggtgcccttg tcctcgatct tgcggcgcag cttgtcgtag tagatcgcgt
     3661 agacctcctc gcgcgtggac gggctgatgt agcgccggcg cgggcgggag ttctcctcgt
     3721 tgatccgctt ctcgatcgcg gcgagcaggc gcagcttctg ctggcgcagt tcctcggtct
     3781 cgcgctcggc ctgcgtcatc gtggccttgc gcgggtcggg cacgggcatc gcggcgatgt
     3841 cgcggcgcac ggtggccagg agctgttgct gctcctgctc gtcggtctgg atcttgcgcg
     3901 acgcctcggt ctggtcgtcg ccgacgagct cgttgggcga cggcggcagc ggcgacgtgg
     3961 cgagcccggc ctcggcctcg ccgccgccgg cgaggttggc ctgcgcgatc gcctgcgcct
     4021 tggtgggcgc ctcgttggag cgcgcgttga ccagcaccac ttccagcggc gtgtcctgga
     4081 acacgcggtt gaagtcctgc ggatcgacga tgcgcacgcc cagcaccgcg gcgtgcagcg
     4141 cgatcgagat cgtcagcgcc agctgcatca cgctcggccg ccacttgcgg atgcgcaccg
     4201 acagcggctc ggcggcgaac atctggcgtt gcggcgggcg tttgaagttc ttgtccatgc
     4261 ggctccgggc ggcgggcgtg tcaggcgggc gtggcggcgc cttcggcggg cggggtctct
     4321 tcggtgacgt cgatggccag cgtgagcggg ccggcgcccg cctcctcggc gtcgtccggc
     4381 tcgtcggcgg cggcctcggc cgcagcgctc tcgtcatcga ggcgcgcgac caggcgggca
     4441 tgcacgtcca gcgtcagcag gtcgacgccg tccacgcgca cccgcacgtg cgtgccgcgc
     4501 gcgaggccgt cgcaaccgat ggccttgaac acgagcggca gcgtgtccgc gcgcaccagg
     4561 ccttccttga gcacggtggc gtcgagctcg gcgacgtcgt tctgctgcag ccagcgcagc
     4621 gtccagaagc gctcgatgcc ggtctggaag ccgttgtagg ccgagtacgc ggtgtcgaag
     4681 cccgagatga cggagaacag cgtcgcgtcc ttgggcttga acggcgcgga cagcggcgcc
     4741 atgcggccgt tgcgcacgca ggcgatgatc tgccactggt tgacgaggtc cacgtagcgc
     4801 cgcagcggcg acgtcgccca ggtgtactgc gccacgccca tgccggcgtg cggcgcgggc
     4861 ttggtgccca tgcgcacctt cacgcccggc gccaggctgg cctggctgcg gtagatgccc
     4921 ggcacgccca tctcggcgat ccagccgccc caggtgctgt tggccaggat catcgcctcc
     4981 gagacgatga ggtccagcgg cgcgccgcgg cggcgttcgc tgatggtgac cgtctcgtcg
     5041 ccggccggct cgctggcggg cgaccggcct tcggcggcct gcaggcggaa gttgtagtcg
     5101 ggccggttga agttctcggg cttgccgcgc accacctcgc gcagcgcctt caggtggcgc
     5161 gcgaggcgga acgtgaacgc gagctcggtg gcgaacgcgt agtcggccgg cgcctcgccc
     5221 agcagcgacg cctcggtgac gtcggcgtcg agcttgtcgt ggcgcaggtt cgcgacgatg
     5281 ggcacgcgct cgacccgggt ctcgctgccc ctgatctcca gcgtggcctc gtcgaaggtg
     5341 acgtacaggc tcagcgccgg gcagtcgcgg ccttcggcga gcgtgtactt ctcgacgatg
     5401 gcgtcgggca gcatcgtgat cttccagccc ggcatgtaga ccgtggagag gcggtcgcgc
     5461 gccaccttgt cgacggggct gtccggcagg atcgccaggc ccggcgcggc gatgtgcacg
     5521 ccgaagacga cgctgccggt gcccaggccg cgcacggata g
//
LOCUS       DATCRO010000357         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_116323, whole genome shotgun sequence.
ACCESSION   DATCRO010000357 DATCRO010000000
VERSION     DATCRO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116323"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..337)
                     /locus_tag="VIN75_09830"
     CDS             complement(92..337)
                     /locus_tag="VIN75_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8974506.1"
                     /translation="MIVCVCHRVSDRDIEREARHGCASFEELQDELRVATACGRCHDC
                     AKDTFDAARACRPTAVEALAHAVARAIPIASVAPLAA"
     gene            complement(778..1254)
                     /gene="bfr"
                     /locus_tag="VIN75_09835"
     CDS             complement(778..1254)
                     /gene="bfr"
                     /locus_tag="VIN75_09835"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY8974507.1"
                     /translation="MKGDPQVIAHLQAQLKNELTAINQYFVHYRMFKHWGFDKIAKKE
                     YDESIGEMKHADKLMDRIFMLDGLPNLQDLGKLLIGETLLEAMECDLKLERAAQATIK
                     DGIAACEVARDYVSRELLQDILDDTEEHIDFLETQLDVAAKVGEQNYLQSMMGEIE"
     gene            complement(1318..1869)
                     /locus_tag="VIN75_09840"
     CDS             complement(1318..1869)
                     /locus_tag="VIN75_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY8974508.1"
                     /translation="MPKTLSPKKAAAAKVRRGAAIATPSDLGAKATKDIAGALNAILA
                     DVFAIYLKTKNFHWHMSGPHFRDYHLLLDDQADQIYAMTDPIAERVRKVGGNTLRSIG
                     HIARLQRVSDNDADFVGPLDMLAELHQDNQQLATSLRAAHEVCDEHRDIATASLIENW
                     IDETERRAWFLFEASRNADTGGH"
     gene            2120..2776
                     /locus_tag="VIN75_09845"
     CDS             2120..2776
                     /locus_tag="VIN75_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974509.1"
                     /translation="MTQVRSSGGALAPFATPVSKGKAKAAAQPEKKIAVPSAAQPRAV
                     GPVASANAPRPGTHVSFGDEALHALAATGQFIGKAVLVGVEDVGEAAYYTAKALGNGV
                     IDVAKGLESGVVDAAKGVGAGVADTVTAIGHGIVHVENAAADVWGVATQGASAVTSLA
                     TTLGADAEVAVTDVGTAATDVGAGAKAVLSGVGSIASTAANYGTYIVKQGGKVLNELT
                     "
     gene            complement(2790..3230)
                     /locus_tag="VIN75_09850"
     CDS             complement(2790..3230)
                     /locus_tag="VIN75_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8974510.1"
                     /translation="MKYLPFVSAELGEPARRGTRWQRFALWLVFAWFLLGGLAHFAFT
                     DLEARIVPPQVPDAPDVVLVVGFFELLGAFGLLLPWTRRAAGWGLLLLTLAVTPANVY
                     MLRIHDQFPSIPVWLLWLRLPLQVVLLWLIWWGSRWRKSARHWY"
     gene            3684..>3921
                     /locus_tag="VIN75_09855"
     CDS             3684..>3921
                     /locus_tag="VIN75_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY8974511.1"
                     /translation="MHRPDFVFGAATASYQIEGAAREDGRGPSIWDTFSRTPGKVFAG
                     HTGDVACDHYHRYVDDVALMAELGLASYRFSIAWP"
BASE COUNT          600 a         1344 c         1389 g          588 t
ORIGIN      
        1 ggttacgcct acggctaacc cgccctacag ggacggtctc gtagggcggg tcaggcgaag
       61 ccgtcacccg ccgttcgccc cccggcgccg atcaggccgc cagcggcgcc acggacgcga
      121 tcgggatcgc gcgcgcgacc gcatgggcca gcgcctcgac cgccgtcggc cggcaggccc
      181 gggcggcgtc gaacgtgtcc ttggcgcagt cgtggcaacg cccgcaggcg gtcgcgacgc
      241 gcagctcgtc ctgcagttcc tcgaacgagg cgcagccgtg gcgcgcctcg cgttcgatgt
      301 cgcggtcgga aacccggtgg cagacgcaca cgatcacagg ccgatctcct gctcggcggc
      361 ggacggggaa acgacgttgg acagcattcg gacgttccgg tggcggtggc tacggcgtaa
      421 tcctaacgcg aatcgttcgc attcacaaga gcaagaacaa ggcgtttttg ccggccagtc
      481 ccaccccgca ggctggcgcc gggtctgacg gaggggtgtg gcctctcctc atgtcgccgc
      541 cacgacgtcg ccatcgtcga gcatgtcgtc ggcgacgtga ggcgaaggcc tgacctcgat
      601 caccagccga tgatcgaggt caggccttcg cctcgaatcg cggtgaccac ctcggctgat
      661 ggcagcatcg cggcgcgatt tcgaggagag gccagacccc tccgggccca gccagcggcg
      721 gtcccaaacg aaacgcgcgg ccggggccgc gcgttcggga tcgagggcgc gagcgtccta
      781 ttcgatctcg cccatcatcg actgcaggta gttctgctcc ccgaccttgg ccgcgacatc
      841 cagctgggtc tccaggaagt cgatgtgctc ttccgtgtcg tccaggatgt cctgcagcaa
      901 ctcgcgcgag acgtagtcgc gcgccacctc gcaggcggcg atgccgtcct tgatcgtcgc
      961 ctgggccgcg cgctcgagct tcaggtcgca ctccatcgcc tcgagcagcg tctcgccgat
     1021 cagcagcttg cccaggtcct gcaggttggg caggccatcg agcatgaaga tgcggtccat
     1081 cagcttgtcg gcgtgcttca tctcgccgat cgactcgtcg tattccttct tggcgatctt
     1141 gtcgaagccc cagtgcttga acatccggta gtggacgaag tactggttga tggccgtcag
     1201 ctcgttcttc agctgcgcct ggaggtgcgc gatgacctgc gggtcgcctt tcatgggagt
     1261 gctcctcgtc gtcggtggtc ggggacggcg cccatcctag cgcggccgcg gccgcgttca
     1321 atgcccgccg gtgtcggcgt tgcggctggc ctcgaacagg aaccaggcgc ggcgctcggt
     1381 ctcgtcgatc cagttctcga tcaggctggc cgtggcgatg tcgcggtgct cgtcgcacac
     1441 ctcgtgcgcg gcgcgcaggc tggtggccag ttgctggttg tcctggtgca gctcggcgag
     1501 catgtccagc gggcccacga agtcggcgtc gttgtccgac acgcgctgca gccgggcgat
     1561 gtggccgatc gagcgcagcg tgttgccgcc caccttgcgc acgcgctcgg cgatcgggtc
     1621 ggtcatggcg tagatctggt cggcctggtc gtccagcagc aggtggtagt cgcggaaatg
     1681 cggcccgctc atgtgccagt ggaagttctt ggtcttcagg tagatggcga agacgtcggc
     1741 caggatcgcg ttgagcgcgc cggcgatgtc cttggtggcc ttggcgccga gatcgctggg
     1801 ggtggcgatc gcggcgccgc ggcgcacctt ggcggcagcg gctttcttgg gggacagggt
     1861 cttgggcatg gtcacctcct cgggttggcg gatcgacacc gcggcgcggc gccggccgcg
     1921 caaggttacc cgcgcgctaa ccaccctgtc gtaggacggg cactcgacga ggagggccga
     1981 attggccgtc cccccgccgc ttatccgcgg ttcgagacag tgtgtcggcg ttgatatagc
     2041 ggggcgattg ccgcgctcat cggtcgatcc acggcccggc caatgcctaa tatcgaggca
     2101 tcgaaaccgg agtcagacca tgacccaagt ccgctcgtcc ggtggcgcgc tggccccctt
     2161 cgccactccc gtgtcgaaag gaaaggccaa ggcggccgcc cagcccgaaa agaagatcgc
     2221 cgtcccctcg gctgcgcagc cgcgcgcggt cggcccggtg gccagcgcca acgcgcccag
     2281 gcccggcacg cacgtgagct tcggcgacga ggcgctgcac gcgctggcgg ccacagggca
     2341 attcatcggc aaggcggtgc tcgtgggcgt cgaggacgtc ggcgaggccg cgtactacac
     2401 ggccaaggcg ctgggcaacg gcgtcatcga cgtcgccaag ggcctggaga gcggggtcgt
     2461 cgatgccgcc aagggcgtgg gcgccggcgt ggccgacacc gtcaccgcga tcggccacgg
     2521 catcgtgcat gtcgagaacg cggccgccga cgtctggggc gtcgcgacgc agggcgcgtc
     2581 ggccgtcacc agcctggcca ccacgctggg cgccgacgcc gaggtcgcgg tcaccgacgt
     2641 cggcaccgcc gccacggacg tgggcgccgg cgccaaggcc gtgctgtcgg gcgtcggcag
     2701 catcgcgagc acggcggcca actacggcac gtacatcgtc aagcagggcg gcaaggtcct
     2761 caacgagctg acctgagggc cggcgcgcct cagtaccagt gccgcgcgga cttgcgccag
     2821 cggctgcccc accagatcag ccacagcagc acgacctgca gcggcaggcg cagccacagc
     2881 agccagaccg ggatgctcgg gaactggtcg tggatgcgca gcatgtagac gttggccggc
     2941 gtcaccgcca gcgtcagcag caagagcccc cagccggcgg cgcgccgcgt ccatggcagc
     3001 agcaggccga acgcgcccag cagctcgaag aagcccacga ccaggacgac gtcgggcgcg
     3061 tcgggcacct gcggcggcac gatgcgcgcc tcgaggtcgg tgaacgcgaa gtgcgcgagc
     3121 ccgcccagca ggaaccaggc gaacacgagc cagagcgcga accgttgcca gcgggtgccg
     3181 cggcgcgccg gctcgcccag ctcggccgag acgaagggca gatacttcat cacgcgccgg
     3241 gccctcccgg tcgagtcggg cggaagtcaa ggcggggcgt cggggatcgc gtcgtcacgg
     3301 gtcgggcggc cgcgcgctcg tggcgcggcg aggcagcgga ggtcaagcag ccaatgatgg
     3361 accacgccct gcccggcctt gggctttcgt gacgaaagaa acgtaacgaa tgacggacgg
     3421 tcgagcaggt cgcaacgctc gtcatccaag cggcgcgcca acgagggtca tgccggagcg
     3481 gcggccgggc cgccaggtca tgcacgaggg cctcggtgtt tccactgata cgcccccctg
     3541 ccctgccgtg cagaatcgcg ccactggaac cggttccacg tcgagggcgc ggaccggtcg
     3601 ggctccccgc ggccacgccg cgcgcaacaa ggcctcactt gagcaacgcg atcgttacca
     3661 tctcgccgcc ggccggctcg gcgatgcacc gcccggactt cgtcttcggc gcggccaccg
     3721 cgtcgtacca gatcgagggc gcggcgcggg aggacggccg cggcccgtcc atctgggaca
     3781 ccttcagccg tacgccggga aaggtcttcg ccgggcacac cggtgacgtg gcgtgcgatc
     3841 actaccaccg ctatgtcgac gacgtggcgc tgatggccga gctgggtctc gcgtcgtacc
     3901 ggttctcgat cgcctggccg c
//
LOCUS       DATCRO010000358         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_161298, whole genome shotgun sequence.
ACCESSION   DATCRO010000358 DATCRO010000000
VERSION     DATCRO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161298"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..516
                     /locus_tag="VIN75_09860"
     CDS             13..516
                     /locus_tag="VIN75_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974512.1"
                     /translation="MTATPDRYTLRPARPADVPAIVGLIGELADFERLSHLMEVTPEK
                     LHPHLFGERPVAEAMVAEHEGGDIVAFALTFVNFSTFLAKPGLYLEDLYVQPAHRGAR
                     LGTRMLTRLAQIAVERGYGRFEWCVLDWNENAIRFYEKMGATIMPDWRLCRVTGESLA
                     QLSERAE"
     gene            complement(590..940)
                     /gene="arsC"
                     /locus_tag="VIN75_09865"
     CDS             complement(590..940)
                     /gene="arsC"
                     /locus_tag="VIN75_09865"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210949.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY8974513.1"
                     /translation="MTVKILHNPRCSKSREALARLESRGLRPEIVDYLATPLSLGELR
                     TLQRQLGVPARAMLRDGEDDYRALGLADASLGDEQLLRAVAEHPKLLQRPVVIDGERA
                     LIARPPELLDDWLK"
     gene            1133..2827
                     /locus_tag="VIN75_09870"
     CDS             1133..2827
                     /locus_tag="VIN75_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8974514.1"
                     /translation="MSDAYALIHGAFRWQVPDRLSIAELCCGRWARETPDAPAIFFDS
                     DSGCRAQYSFGQLQRAANRLSNALRRLGVQAGDRVGLVLPQRFETAVAHIAINQLGAV
                     AMPLSMLFGPDALAYRLQDSEASVAIVESGALAAVRSVRAQCPALRQVLVVGESEPQD
                     GEAGWMAALQAEDARFAARDNAAEDPAILIYTSGTTGAPKGALLPQRALLGNLTGFIA
                     SQNWFGFDPHDPKVRSEAVFWSPADWAWTGGLMDALLPSLCFGRPILGYQGRFSPEKA
                     FELMQHYRVSHTFLFPTALKAMMKAVPHPAEHYALRLEAVMSAGEAVGDAVFDWVRRE
                     LGVTVNEMFGQTEINYVVGNCARLWPAKPGSMGRAYPGHRVAVIDDDGQVCPPGQIGE
                     VAVHRRDGHGDLDPIFFLGYWRNEAATAAKFTGDPLDSWCRTGDLARTDEDGYLWYQG
                     RADDQFKVAGYRIGPGEIENCLVRHEAVANAAVVPKPDAERGALVKAFVVLAPGRQPS
                     AALTGELQDFVRQRLAPYEYPKEIEFLDSLPMTTTGKVQRRVLRQLEADRAAGRVA"
     gene            complement(2850..3704)
                     /locus_tag="VIN75_09875"
     CDS             complement(2850..3704)
                     /locus_tag="VIN75_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974515.1"
                     /translation="MKPQQFQRARLAAALLVTGVLAVGCGPAAMAAPTAATASAAADD
                     PQAGSGPAAAPVALPTVQSDVKRGASFPYKRLNELLSKLRTEGEGLFVSRFVLTDAKD
                     SSKPPPAGTKLALMSDDDYIPIPIDDKGRFDLPTFSERQAKDMDFGTNVPKGELGVRL
                     SIDLATPPEMLDMATVRRIVTVGQRLRTELLPWYVRWMFPQIDGVIACSDAPQWTLDW
                     SEGAQRWRLPLPAEAQQREPETPKGKPSRACTVLTGQEQWPDAALLQTPPGANTKLYV
                     KLRNPRAS"
     gene            complement(3824..>4023)
                     /locus_tag="VIN75_09880"
     CDS             complement(3824..>4023)
                     /locus_tag="VIN75_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974516.1"
                     /translation="QPLLAERADEASRAPDRVDASTKLGNEMMKSASGDCLKGEYAGG
                     GMGLLSAPFWLLAEARGKCRR"
BASE COUNT          539 a         1436 c         1440 g          608 t
ORIGIN      
        1 ccccacgcca ccatgaccgc cacgcccgac cgctacaccc ttcgccccgc ccgccccgcc
       61 gacgtccccg ccatcgtcgg cctgatcggg gaactcgccg atttcgagcg cctgtcgcat
      121 ctgatggagg tcacgcccga gaagctgcat ccgcacctct tcggcgagcg tcccgtcgcc
      181 gaggccatgg tcgccgagca cgagggcggc gacatcgtcg ccttcgcgct gaccttcgtg
      241 aacttctcga ccttcctggc caagcccggg ctctacctcg aggacctcta cgtccagccc
      301 gcccatcgcg gcgcgcgact cggcacgcgc atgctgaccc gcctggcgca gatcgccgtc
      361 gagcgcggct acggtcgctt cgaatggtgc gtgctcgact ggaacgagaa cgccatccgc
      421 ttctacgaaa agatgggcgc gacgatcatg ccggactggc gcctgtgccg cgtcaccgga
      481 gagtcgctcg cgcaactgag cgagcgggcc gagtgagctt gccgagtgag cttgccgaga
      541 gagccgaccc gggcgcggcg cgccgggcac gtgcgcgggc cgggtggcct cacttcaacc
      601 aatcgtcgag cagctccggt ggacgggcga tcagcgcgcg ctcgccgtcg atgacgacgg
      661 ggcgttgcag cagcttcgga tgctcggcca ccgcgcgcag cagttgctcg tcgccgagcg
      721 acgcgtcggc caggccgagc gcgcggtagt cgtcctcgcc atcgcgcagc atcgcgcggg
      781 cggggacgcc gagctgccgt tgcagggtcc gcagctcgcc cagcgacaag ggcgtggcca
      841 ggtagtcgac gatctcgggg cgaaggcccc gcgattcgag ccgggcaagc gcctcgcgcg
      901 acttcgagca gcgggggttg tgcaggatct tcacggtcat ccggcggagt gtaggcggcg
      961 ccttcacgcc gtcatgcgcg caccgaggat cgatccgcgc tttccggtgc accgcgcggg
     1021 ggcacgcccc gcaagaccgg gcaccgtcct ggcgctacgg gctttccatg acgcccggtg
     1081 ccccctcgct cgctagcatc cacgctcttg ttccagtccc cggagagtcg cgatgtccga
     1141 cgcttacgcg ctgatccacg gcgcgttccg ctggcaggtg cccgaccgct tgtccatcgc
     1201 cgaactctgc tgcggccgct gggcccgcga aacacccgac gcgcccgcca tcttcttcga
     1261 cagcgacagc ggttgtcgcg cccagtactc cttcggccag ctgcagcgcg cggcgaatcg
     1321 cctgtccaac gcactgcggc gtctcggcgt ccaggccggg gaccgcgtgg gcctggtgct
     1381 gccgcaacgc ttcgagacgg ccgtcgcgca catcgcgatc aaccagctcg gcgcggtggc
     1441 gatgccgctg tcgatgctct tcggccccga tgcgctggcg taccggctcc aggacagcga
     1501 ggccagcgtc gcgatcgtcg agagcggggc gctcgccgcg gtccgctcgg tgcgcgcgca
     1561 gtgtccggcg ctgcgccagg tgctggtggt cggcgagtcc gagccccagg acggcgaagc
     1621 cggctggatg gcggcgcttc aggcggagga cgcgcgcttc gccgcgcgcg acaacgcggc
     1681 cgaggacccg gcgatcctga tctacaccag cggcacgacg ggcgcgccca agggcgccct
     1741 gctgccgcag cgcgcgctgc tgggcaatct gaccggcttc atcgcgagcc agaactggtt
     1801 cggcttcgat ccccatgatc cgaaggtccg cagcgaggcg gtgttctgga gccccgccga
     1861 ctgggcctgg accggcgggt tgatggacgc gctgctgccg tcgctgtgtt tcggtcgacc
     1921 catcctcggc taccagggtc gcttttcgcc ggagaaggcc ttcgagctga tgcagcacta
     1981 ccgcgtcagc cacaccttcc tgttcccgac ggccctgaag gccatgatga aggcggtgcc
     2041 gcatcccgcc gagcattacg cgctgcgcct ggaggccgtg atgagcgccg gcgaggccgt
     2101 cggcgacgcc gtcttcgact gggtccggcg cgagctgggc gtgacggtca acgagatgtt
     2161 cggccagacc gagatcaact acgtcgtcgg caactgcgcg cggctgtggc cggccaagcc
     2221 gggcagcatg ggccgcgcgt atcccggtca ccgcgtcgcg gtcatcgacg acgacggcca
     2281 ggtctgcccg cccgggcaga tcggcgaggt ggcggtgcat cggcgggacg gacatggcga
     2341 cctcgatccg atcttcttcc tcggctattg gcgcaacgag gccgcgacgg cggcgaagtt
     2401 caccggcgac ccgctggaca gctggtgtcg caccggcgat ctcgcgcgca ccgatgagga
     2461 cggctacctg tggtaccagg gccgcgccga cgatcagttc aaggtcgcgg gctaccgcat
     2521 cggccccggc gagatcgaga actgcctggt gcgccacgag gcggtggcca acgccgcggt
     2581 cgtgcccaag cccgacgccg agcgcggcgc gctggtgaag gccttcgtcg tgctggcgcc
     2641 ggggcggcag ccctcggccg cgttgaccgg cgagctgcag gacttcgtcc ggcaacggct
     2701 cgcgccctac gagtacccca aggagatcga gttcctcgac agcctgccga tgaccaccac
     2761 cggcaaggtg cagcgccgcg tcctgcggca gctcgaggcg gaccgggccg ccgggcgggt
     2821 ggcttgagcc ccgcgcggcg ggccgtcgct cagctggccc gcggattgcg cagcttgacg
     2881 tagagcttgg tgttggcgcc gggcggcgtc tggagcagcg cggcgtcggg ccattgctcc
     2941 tggcccgtca gcacggtgca ggcgcgcgag ggcttgccct tgggcgtctc cggctcgcgc
     3001 tgctgcgcct ccgccggcag cggcaggcgc cagcgctgcg ccccctcgga ccagtccagg
     3061 gtccactgcg gcgcgtcgct gcaggcgatc acgccgtcga tctgcgggaa catccagcgc
     3121 acgtaccagg gcagcagctc ggtgcgcaga cgctggccga cggtgacgat gcgccgcacc
     3181 gtggccatgt ccagcatctc cggcggcgtg gccagatcga tgctcagccg cacgccgagc
     3241 tcgcccttgg gcacgttggt gccgaagtcc atgtccttgg cctggcgctc gctgaaggtc
     3301 ggcaggtcga agcggccctt gtcgtcgatc gggatgggga tgtagtcgtc gtcgctcatc
     3361 agcgcgagct tggtgcccgc gggtggcggc ttgctcgaat ccttcgcgtc ggtcagcacg
     3421 aagcggctga cgaagagccc ttcgccttcg gtgcgcaact tcgacagcag ctcgttcagg
     3481 cgcttgtagg ggaaggaggc gccgcgcttg acgtcgctct ggacggtggg cagggcgacc
     3541 ggcgccgcgg ccgggccgga gcctgcctgc gggtcgtccg ccgcggcgga tgcggtggcc
     3601 gcggtgggcg cggccatcgc cgccgggccg cagcccacgg cgagcacgcc ggtcacgagg
     3661 agcgcggccg cgaggcgcgc gcgctggaac tgctggggtt tcacggttct ctctccctgg
     3721 tcgctgtgag cgatccgcgc tgaacggcgc gcgatcggat tgcgttgacg acggctgtcc
     3781 ctcatcgggg tggcaagctc gcggtgccgc ggcgaggcgg agctcagcgc cggcacttgc
     3841 cgcgcgcctc ggcgagcagc cagaacggcg cgctgagcag ccccatgccg ccgccggcgt
     3901 attcgccctt gaggcaatcg ccactggcac tcttcatcat ctcgttgccg agcttggtcg
     3961 aggcatcgac ccggtccggc gcgcggctgg cctcgtcggc ccgctcggcc agcagcggct
     4021 ggc
//
LOCUS       DATCRO010000359         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_622677, whole genome shotgun sequence.
ACCESSION   DATCRO010000359 DATCRO010000000
VERSION     DATCRO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622677"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VIN75_09885"
     CDS             complement(<1..180)
                     /locus_tag="VIN75_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974517.1"
                     /translation="MPGIRLSDRLLQRVHRFVAARPYFTGSIVMHALLALLLLNLPGL
                     GARQDARVAAAEASKA"
     gene            complement(349..936)
                     /gene="recR"
                     /locus_tag="VIN75_09890"
     CDS             complement(349..936)
                     /gene="recR"
                     /locus_tag="VIN75_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8974518.1"
                     /translation="MTEPRLLSLVEALRILPGVGVKSAQRMAFHLLQHDRDGALRLAA
                     ALDGAVHDVRHCERCHTFTEHRLCDVCADPERDANVLCIVETPADQSALERTGSYKGL
                     YFVLMGRLSPLDGVGVRDIGVAPLLERIEREPVNEVILATSFTAEGEATAHVIGEALK
                     ARGLTVSRLARGVPAGSELEYVDLSTIAHALADRR"
     gene            complement(1072..1398)
                     /locus_tag="VIN75_09895"
     CDS             complement(1072..1398)
                     /locus_tag="VIN75_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8974519.1"
                     /translation="MMKNQLAGLMKQAQAMQDNLKKVQDELATIEVDGQAGNGLVKVT
                     MTCKHEVRRVTIDPSLLADDKDMLEDMVAAAFNDALRVAEELSSAKMSKVTQGMPLPP
                     GMKMPF"
     gene            complement(1639..>2165)
                     /locus_tag="VIN75_09900"
     CDS             complement(1639..>2165)
                     /locus_tag="VIN75_09900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023780.4"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8974520.1"
                     /translation="PVQRARPSLDELEPEYDDEPGAPAPAPQGAAPPPALPPFERTEL
                     GTRWYDLINQCAAAGKLVAMVRALAMQAELVHCDPAGEAPTWRLRVALETLRNPALAD
                     KLGAILRELTGQNLQLVVEMGEPQDTPLKRDAAEADRRQRAAIDEIHSDATVRALLAQ
                     FQTARILPGSVKPL"
BASE COUNT          298 a          733 c          811 g          323 t
ORIGIN      
        1 ggccttgctc gcctcggccg cggccacgcg cgcgtcctgg cgcgcgccca ggcccggcag
       61 gttcagcagc aacagcgcga gcagcgcgtg catgacgatc gagcccgtga aatatggccg
      121 cgccgcgacg aagcggtgca cgcgttgcag caggcgatcg ctgagacgga tcccgggcac
      181 gcgaatgccg gcgatcgggg gcgaggactg ggtcggggtc aacgacggag gctctccgga
      241 cacgcgcacc gcggcagtcg gcagtgcgtt tcgattgtgt ccaggagccg cgccgggcgc
      301 agcgccggtg tccgctatcg gacaccccgg gccgtcacgg gcgcgacgtc aacggcggtc
      361 ggccaaggcg tgcgcaatcg tcgacaggtc gacgtactcc agctcgctgc cggccggcac
      421 gccgcgcgcc aggcgcgaca ccgtcaggcc gcgcgccttc agcgcctcgc cgatcacgtg
      481 cgcggtggcc tcgccctcgg cggtgaagct ggtggccagg atcacctcgt tgaccggctc
      541 gcgctcgatg cgttcgagca gcggcgcgac gccgatgtcg cgcacgccga cgccgtcgag
      601 cggactcaac cgccccatca gcacgaagta caggcccttg tagctgccgg tgcgctcgag
      661 cgccgactgg tcggccggcg tctcgacgat gcacagcacg ttcgcatcgc gctcgggatc
      721 ggcgcagacg tcgcacaggc ggtgctcggt gaaggtgtgg cagcgctcgc aatggcgcac
      781 gtcgtgcacc gcgccgtcca gcgcggcggc gaggcgcagc gcgccgtcgc ggtcgtgctg
      841 aagcaggtgg aaggccatgc gctgcgccga cttcacgccg acgcccggca ggatgcgcag
      901 cgcctccacc aatgacagga gccgtggctc ggtcatgggc aattgccttc agacccggcg
      961 ctgccgctcg caaggtggcc gggagagtcg agggagcgcg ggaaccggct ccgccgggcc
     1021 cgtcgcggcc ggccccctcg gggggcagcg accagcggga gcgtgggggt cctagaacgg
     1081 catcttcatg ccgggaggca gcggcatgcc ctgcgtcacc ttgctcatct tggccgagct
     1141 cagctcctcg gcgacgcgca gggcgtcgtt gaaggcggcg gcgaccatgt cctcgagcat
     1201 gtccttgtcg tcggccagca ggctcgggtc gatcgtgacg cggcgcacct cgtgcttgca
     1261 ggtcatggtg accttgacga ggccgttgcc ggcctggccg tcgacctcga tcgtggcgag
     1321 ctcgtcctgc accttcttca ggttgtcctg catcgcctgg gcctgcttca tcaggccggc
     1381 gagttggttc ttcatcatgg ggtggacctt tcggggaacg tgggggcgct cgcgcgaccc
     1441 cggagggaaa tctagtgtcg tgagttggaa attcgttgaa taggcctgga gcgaaggagt
     1501 gacggtgccg aattccgtcc agaacggttg cagactaggc gcgcccgcga cacgatgccg
     1561 ggcggcctcg cgagcgggcg caacgacgga tgcggccgtt ctgggcggaa ttcttcgacg
     1621 aatttccaac tcacgacact agaggggctt gaccgagccc ggcaggatgc gggcggtctg
     1681 gaactgggca agcagcgcgc gcacggtggc gtcggaatgg atctcgtcga tcgcggcgcg
     1741 ctggcggcgg tccgcctcgg cggcgtcgcg cttgagcggc gtgtcctgcg gctcgcccat
     1801 ctccacgacc agttgcaggt tctggcccgt gagctcgcgc aggatggcgc ccagcttgtc
     1861 ggccagcgcc gggttgcgca gcgtctcgag cgccacgcgc aggcgccagg tgggcgcctc
     1921 gccggcgggg tcgcagtgca cgagctcggc ctgcatcgcc agcgcgcgca ccatcgcgac
     1981 cagcttgccg gcggcggcgc actggttgat caggtcgtac cagcgcgtgc cgagctcggt
     2041 gcgctcgaac ggcggcagcg ccggcggcgg cgcggcgcct tgcggcgcgg gcgccggggc
     2101 accgggctcg tcgtcgtact cgggctcgag ttcgtccagg ctgggacgcg cgcgctgcac
     2161 cggcg
//
LOCUS       DATCRO010000360         5545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_915296, whole genome shotgun sequence.
ACCESSION   DATCRO010000360 DATCRO010000000
VERSION     DATCRO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915296"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VIN75_09905"
     CDS             complement(<1..462)
                     /locus_tag="VIN75_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974521.1"
                     /translation="MHRLPPLAAVVLANWIALASAATLVADPPEVFAPGVVSGPSNDA
                     DACVTPDGSALVFARDGAILVAERTPAGWSTPRVAPFSGRWMDAQPTLAPDGTALVFV
                     SNRPLAAGDAKHPSGNLWRVERHGGAWGEPVHLPAAVNRGPGVWGPSVAADG"
     gene            725..2023
                     /locus_tag="VIN75_09910"
     CDS             725..2023
                     /locus_tag="VIN75_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEY8974522.1"
                     /translation="MRLKTWTAVAAAITLPLISGCGNDNANDGFVRNINATSEYTSLN
                     LYTQDSDGSDAILISATAANTASAYEGLKKGSYTFDVKSSTSAGSPTPVTGTVTKDDH
                     FAVLTYLTGTTTKTQFISEEEAHPASGNAKLRVFNAATSEAASVDVYLTTHACDALAG
                     TDSPFASAVTALQTSYTQVTASTWNVCVFATGDTSTLLLDVEGLKLADQEIATLVLTR
                     TTGGVLLNGAVLDQQGAYTTYASAIARVRVVADASGGTPVNVTIGSSEIATSQPSPSV
                     ANYVTVPTGTLSPTIVYGSTSTSGYTLPAVAAGNDYTLLVAGSAGAPAVTLITDNNTP
                     STSTTNTVKARVINGLNGTNGAVSATVDGKSVGSASFGAASTSYTSILPTSGTSTIHG
                     ITTGTNPTDLVNQTFSAGSVYTIFIYGDATAPTMAQVIDR"
     gene            2055..2837
                     /locus_tag="VIN75_09915"
     CDS             2055..2837
                     /locus_tag="VIN75_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8974523.1"
                     /translation="MSDAHIRLEPADAAGVAILRIANPGKMNALSLAMWRELRAVFDA
                     LRDDASPPRVIVVRGADREFVAGADIEEFPQFRFEPESLAAYHEDIVAPALRAMLDCD
                     VPLIAHIEGACIGGGLEIAACCDLRLCEPSSRFGVPIARLGFPMAPAEVEIVSHIVGQ
                     TLLRELLIESRLMGAAEAHERGVVTRVVDASGLEAEVRRVALHIAGLSAQAMRLNKRV
                     LRAFARPTFSEPEDRAAHYAYADSDEHREGLQAFMDRRPPNF"
     gene            3075..4640
                     /locus_tag="VIN75_09920"
     CDS             3075..4640
                     /locus_tag="VIN75_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEY8974524.1"
                     /translation="MANANLYARLRPHFPARLDDVAIETADAPVGHDGHAFTPLRYTW
                     RDLERGSARIANLLADLALPAGARVLAHVDKSVEAMMLYLATLRAGLVFVPLNTAYQA
                     SEVEYFVENASPDVVVCAPRNFPWISRIAFARGTRMVLTLGDDRSGSLLERAAQMGDE
                     FETVARAPGDVAAILYTSGTTGRSKGAQLTHGNLLANADTLHRFWHWKEGEQRDVLIH
                     ALPIFHIHGLFVALHGMLRAGARLVWFHRFDPLGVIARLPDATMFMGVPTLYVRMLAQ
                     AALGPEACKGMRLFISGSAPLLADTFDAWRERTGHTILERYGMSETVMITSNPYRPES
                     ARQAGTVGVALPGVGVRVQDDQGRDCPPGEIGNVQVQGPSVFAGYWQMPEKTREEFTA
                     DGWFRTGDVGKLDAHGVLTLIGRSKDLIISGGFNVYPAEIEGLINDMAGVAESAVVGV
                     PHPDFGEAVVAVVVARAGAALVPGQIVAALKSSIANFKVPKHVRVVEALPRNTMGKVQ
                     KKLLREELAALFA"
     gene            4637..4858
                     /locus_tag="VIN75_09925"
     CDS             4637..4858
                     /locus_tag="VIN75_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEY8974525.1"
                     /translation="MSSRDMPEGWRAAACPRCGGAFECGAGADRATPCFCVSFALGDA
                     RLAELRAKWSDCLCAACLAALAADPELPA"
     gene            4855..>5545
                     /locus_tag="VIN75_09930"
     CDS             4855..>5545
                     /locus_tag="VIN75_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8974526.1"
                     /translation="MTSDLRLVTALTLDLDDTLWPVRPTLVAAEQVLAGWLREHAPAT
                     AQGMPPAAMLALRAEVAAEHPHWGHDLSAIRLETIRRALRRHGDDPALAEVAFEVFFE
                     ARHAVTLYDDVLPGLERLASRHRLVAVSNGNAELHRVGLDRFFAGSVSARLHGVAKPD
                     PSIFRAACEAAGATPAQVLHLGDDLDADVGGALAAGLHAGWVCRPDGAHASEAPRDGA
                     HRFADLIEVALA"
BASE COUNT          824 a         2072 c         1933 g          716 t
ORIGIN      
        1 gccgtcggcg gcgacgctgg gcccccagac gccgggcccg cgattgacgg cggccggcag
       61 gtgcaccggc tcgccccagg cgccgccgtg ccgctccacg cgccacaggt tgcccgacgg
      121 atgcttcgca tcgcccgcgg ccagcggccg gttcgacacg aacacgagcg cggtgccatc
      181 cggcgccagc gtgggttgcg cgtccatcca gcggccggag aacggcgcga cgcgcggcgt
      241 cgaccagccc gccggcgtgc gctcggcgac gaggatcgcg ccatcgcgcg cgaacaccag
      301 cgccgagcca tcgggcgtga cgcacgcgtc ggcgtcgttc gacggccccg acaccacgcc
      361 gggcgcgaag acctccggcg gatcggccac gagcgtggcg gcggacgcga gggcgatcca
      421 gttcgccagg acgacggcgg cgagcggcgg caggcgatgc atgggcggtc cttctcgtga
      481 aggccgccat cgtcgccagg ccggcgcgcg gccgcagccc cgatgcgacg aagcacggaa
      541 agccgggccc gagcggcggc ggccgggccc atccccgcgc cgcactacac tgcgacatcg
      601 gccacgatgt cgcgccggcc acgtccggat cgccaagatc gagcaacaaa ccggcacggt
      661 cgcgcaggca cactgcgcgc cacgcgccgc gagcggccgc acaacgacat tcacggagta
      721 gacgatgcgc ctgaagacct ggaccgccgt ggcggccgca atcaccctgc cgttgatcag
      781 cggctgcggc aacgacaacg ccaacgacgg cttcgtgcgc aacatcaacg ccacgagcga
      841 atacacgagc ctgaacctct acacccagga cagcgacggc agcgacgcga tcctgatcag
      901 cgcaaccgcg gccaacacgg ccagtgcgta cgaaggcctc aagaagggca gctacacctt
      961 cgacgtcaag agctccacca gcgccggctc gccgacgccc gtcacgggca ccgtcaccaa
     1021 ggacgaccac ttcgccgtcc tcacctacct caccggcacg accaccaaga cccagttcat
     1081 ctcggaggag gaggcgcacc ccgcctcggg caacgcgaag ctgcgcgtct tcaacgccgc
     1141 caccagcgaa gccgcgagcg tcgacgtcta cctgaccacc catgcgtgcg acgcgctggc
     1201 cggcaccgat tcgcccttcg cctcggccgt gacggcgctg cagaccagct acacgcaggt
     1261 caccgcctcg acctggaacg tgtgcgtgtt cgccaccggc gacacgagca cgctcctgct
     1321 ggacgtcgag ggcctgaagc tcgccgacca ggagatcgcc acgctggtgc tgacccgcac
     1381 cacgggcggc gtgctgctca acggcgccgt gctcgaccag cagggcgcgt acacgaccta
     1441 tgccagcgcg atcgcccgcg tgcgcgtggt cgcggacgcc tcggggggca cgccggtcaa
     1501 cgtcacgatc ggcagcagcg agatcgccac gagccagccc tcgccgtccg tcgccaacta
     1561 cgtgaccgtg ccgaccggca cgctgtcgcc gaccatcgtg tacggcagca ccagcacgag
     1621 cggctacacg ctgccggcgg tggcggcggg caacgactac accctgctgg tcgcgggcag
     1681 cgccggcgcg ccggccgtca cgctcatcac ggacaacaac acgccgtcca ccagcacgac
     1741 caacaccgtc aaggcccgcg tcatcaatgg cctgaacggc accaacggcg cggtgtcggc
     1801 gacggtcgac ggcaagtcgg tcggctctgc cagcttcggc gccgcatcga cgagctacac
     1861 gagcatcctg cccaccagcg gcacatccac catccacggc atcacgacgg gcacgaaccc
     1921 gaccgacctc gtcaaccaga cgttctcggc aggcagtgtc tacacgatct tcatctacgg
     1981 cgacgccacc gccccgacga tggcccaggt catcgatcgc tgacggcggc gcctcccggc
     2041 cacggaggct ccccatgtcc gacgcccaca tccgactcga gccggccgac gcggccggcg
     2101 tcgccatcct gcgcatcgcc aaccccggca agatgaacgc gctgagcctg gcgatgtggc
     2161 gcgagctgcg cgccgtgttc gacgcgctgc gcgacgacgc gtcgccgccg cgggtgatcg
     2221 tggtgcgcgg cgcggaccgc gagttcgtcg cgggcgccga catcgaggag ttcccgcagt
     2281 tccgcttcga gcccgagtcg ctcgcggcat accacgagga catcgtcgcc ccggccctgc
     2341 gcgccatgct cgactgcgac gtgccgctca tcgcgcacat cgagggcgcg tgcatcggcg
     2401 gcgggctcga gatcgcagcc tgctgcgacc tgcggttgtg cgagccgtcc agccgcttcg
     2461 gcgtgccgat cgcgcgcctg ggcttcccga tggcgccggc cgaggtcgag atcgtctcgc
     2521 acatcgtcgg ccagacgctg ctgcgcgagc tgctgatcga gtcgcgcctg atgggcgccg
     2581 ccgaggcgca cgagcgcggc gtcgtcacgc gggtcgtcga cgcgagcggg ctcgaggccg
     2641 aggtgcgccg cgtcgcgctg cacatcgccg gcctgtcggc acaggccatg cgcctgaaca
     2701 agcgcgtgct gcgcgcgttc gcacggccca ccttcagcga gcccgaggat cgcgccgcgc
     2761 actacgcgta tgccgattcg gacgagcacc gcgagggcct gcaggcgttc atggataggc
     2821 gcccaccgaa cttttgaccc ccacgctcgc tgttcccggg aggggatgct tcgcgagcgc
     2881 tcgctgcccc cgagggggcg ctccgcgacg cgcccggcag agccggatcg cgcgctccct
     2941 tgaacgttgt gcgtgaggcc cgagcggccg cgccgggccg gcgagggctt cgcactccgt
     3001 tcggattcag gcccgggcgg gctgagcacg gccggcgcaa aatcgcaggt tccgtattgc
     3061 gtattcactt gcccatggcc aacgccaacc tctacgcccg gctgcgcccg cacttccccg
     3121 cgcggctgga cgacgtcgcc atcgagaccg ccgacgcgcc cgtcgggcac gacgggcacg
     3181 ccttcacgcc gttgcgctac acgtggcgcg acctcgagcg gggcagcgcg cgcatcgcca
     3241 acctgcttgc cgacctcgcg ctgcccgcgg gcgcgcgcgt gctcgcgcac gtggacaagt
     3301 cggtcgaggc gatgatgctg tacctggcca cgctgcgcgc ggggctcgtc ttcgtgccgc
     3361 tgaacacggc ctaccaggcc agcgaggtcg agtacttcgt cgagaacgcg tcgcccgacg
     3421 tggtggtgtg cgcgccgcgc aactttccgt ggatcagccg catcgcgttc gcgcgcggca
     3481 cccgcatggt gctcacgctg ggcgacgacc gctcgggcag cctgctggaa cgcgcggcgc
     3541 agatgggcga cgagttcgag acggtggcgc gcgcgccggg cgacgtcgcc gccatcctct
     3601 acaccagcgg caccaccggc cgcagcaagg gcgcgcagct gacgcacggc aacctgctgg
     3661 ccaacgccga cacgctgcac cgcttctggc actggaagga aggcgagcag cgcgacgtgc
     3721 tgatccacgc gttgccgatc ttccacatcc acggcctgtt cgtggcgctg cacggcatgc
     3781 tgcgcgccgg cgccaggctc gtctggttcc acaggttcga cccgctgggc gtcatcgcgc
     3841 gcctgcccga cgccacgatg ttcatgggcg tgcccacgct gtacgtgcgc atgctggcgc
     3901 aggccgcgct cgggcccgag gcgtgcaagg gcatgcgcct gttcatcagc ggctcggcgc
     3961 cgcttctggc cgacaccttc gacgcctggc gcgaacgcac cggccacacg atcctcgagc
     4021 gctacggcat gagcgagacg gtgatgatca cgtccaaccc gtaccgtccc gagagcgcgc
     4081 gccaggcggg caccgtgggc gtcgcgctgc ccggggtggg cgtgcgcgtg caggacgacc
     4141 agggccgcga ctgccccccg ggcgagatcg gcaacgtcca ggtccagggc cccagcgtgt
     4201 tcgccggcta ttggcagatg cccgagaaga cgcgcgagga gttcaccgcc gacggctggt
     4261 tccgcaccgg cgacgtcggc aagctggacg cgcacggcgt gctgacgctg atcggccgca
     4321 gcaaggacct gatcatcagc ggcggcttca acgtctatcc ggcggagatc gagggcttga
     4381 tcaacgacat ggccggcgtg gccgaatcgg ccgtcgtcgg tgtgccgcat ccggacttcg
     4441 gcgaggccgt cgtcgccgtg gtggtggcgc gcgccggcgc ggcgctggtg cccggacaga
     4501 tcgtcgcggc gctgaaatcg tccatcgcca acttcaaggt gcccaagcac gtgcgcgtgg
     4561 tcgaggccct gccccgcaac acgatgggca aggtccagaa gaagctgctg cgcgaggagc
     4621 tggcggcgct gttcgcatga gctcgcgaga catgcccgag ggctggcgcg cggccgcctg
     4681 cccgcgctgc ggcggcgcgt tcgaatgcgg cgcgggcgcc gatcgcgcga caccctgctt
     4741 ctgcgtgtcg ttcgcgttgg gcgacgcgcg cctggccgag ctgcgcgcga agtggtccga
     4801 ctgcctgtgc gccgcctgcc tggcggcgct cgccgcggac ccggagcttc cggcatgacc
     4861 agtgacctgc gcctcgtcac cgcgctgacg ctcgatctgg acgacacgct gtggcccgtg
     4921 cgtccgacgc tggtggccgc cgaacaggtg ctcgccggct ggctgcgcga gcacgcgccg
     4981 gccaccgcgc agggcatgcc gcccgcggcg atgctggcgc tgcgcgccga ggtcgccgcg
     5041 gagcatccgc actggggcca cgacctcagc gcgatccggc tcgagacgat ccgccgcgcg
     5101 ctgcggcgcc acggcgacga cccggcgctg gccgaggtcg cgttcgaggt cttcttcgag
     5161 gcgcgccacg cggtcacgct gtacgacgac gtgctgcccg ggctcgagcg gctggcctcg
     5221 cgccaccggc tcgtggcggt gagcaacggc aacgcggagc tgcatcgcgt gggactcgac
     5281 cgcttcttcg cgggctcggt gtcggcgcgg ctgcatggcg tggccaagcc ggatccgtcg
     5341 atcttccgcg cggcgtgcga ggccgctggg gcgacgccgg cgcaggtgct gcacctgggc
     5401 gacgacctcg acgccgacgt cggcggcgcg ctggccgcgg gcctgcacgc cggctgggtg
     5461 tgccggcccg acggggcaca cgcgagcgag gcgccgcgcg acggcgcgca tcgcttcgcc
     5521 gacctcatcg aagtggcgct ggcgc
//
LOCUS       DATCRO010000361         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1016184, whole genome shotgun sequence.
ACCESSION   DATCRO010000361 DATCRO010000000
VERSION     DATCRO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016184"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1417)
                     /gene="tolB"
                     /locus_tag="VIN75_09935"
     CDS             complement(143..1417)
                     /gene="tolB"
                     /locus_tag="VIN75_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY8974527.1"
                     /translation="MWPRRHALRALGASLALPAMTPAFAQFLVQIRGVGGTQIPIALG
                     RFRDEDRQPQSISAVVRADLERSGVFKVVDAPAPADENGLPDYNTLRSRGADWVVGGS
                     ASRLADGRVDVRFRLWDVVKGADQGGQSEPTQPADLRLAAHHISDLVYQKVTGERGVF
                     STRIAYVTKGGGKHTLRIADADGANIVPALVDNEPIISPAWSPDGSELAFVTFHDKKA
                     KVVIRNVRTGAMREVAAFKGSNSAPAFSPDGRTLAVTLSKDGGSHVYLMDRNGGNPRR
                     VTESTGIDTEARFSPDGATLFFVSDRGGGPQIYRQPVGGGGAQRVTYNGAYNISPAIS
                     PDGKLLAYISRGDGFKLMVMNLGTGEVASVSDTNDDESPSFAPNGRYIIYATKANGRD
                     VLMVTTTDGRFKSTLVESGLDIREPVWGPFGR"
     gene            1608..>2833
                     /locus_tag="VIN75_09940"
     CDS             1608..>2833
                     /locus_tag="VIN75_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974528.1"
                     /translation="MTTLKQRLARVIPYFRDNSRAALGLAAMAMVVAAATEPMIPYIL
                     KHLLNSGFKDTSGLPLWAIPLAIVVLFMIRGTSWWVSSYGLTKASQNAVLAVRAHMFS
                     HMLRAAPALFTRNTASSITNNIVYEIQQGANLLMGSVQILIRDSLTALFFVGYLVILN
                     WRLTLFVVVLFPIVGLLVRVVSKRLHKLNVAGQNATDDLAYVVEENVLAWRIVRLHGA
                     AAQEQRRFDASSHLMRSLNIKQQASGSLMTPVTQVLASFALAAVLWMALAESSKTGGT
                     PGDFVAFITCMLLLIQPAKHLSDVMAPLTRGTAAMERGLDMMEAVPIEGGGTIAPARV
                     KGDIEFRDVALRYDARDSVEHAQDDAPAEVPAGTPVAALSDITLAIRAGETVAFVGPS
                     GAGKTSLVNLLPRFVEP"
BASE COUNT          420 a          974 c          985 g          454 t
ORIGIN      
        1 gtgggcgagg cgcagcccgc cacgagggcc acgccggcca gcgccagcaa catcttcccg
       61 gaattcttga tcatcgtttc tctcctacca gcactatgaa catggccgcg agcgcccctg
      121 cgccgcggct tcttccccga aatcagcggc cgaacgggcc ccagacaggt tctcggatgt
      181 cgaggcccga ttcgaccagt gtcgacttga atcggccgtc ggtcgtcgtg accatcagca
      241 cgtcgcggcc gttggccttc gtcgcgtaga tgatgtagcg accgttgggc gcgaagctcg
      301 ggctctcgtc gtcgttggtg tcgctgacgc tggccacctc gccggtgccc aggttcatca
      361 ccatcagctt gaagccgtcg ccgcgggaaa tgtatgcgag aagcttgccg tccgggctga
      421 tcgcggggct gatgttgtaa gcaccgttgt acgtgacgcg ctgcgcgccg ccgccgccca
      481 cgggctggcg gtagatctgc gggccgcctc cgcggtcgct gacgaagaac agcgtggcgc
      541 cgtccggcga gaacctggcc tcggtgtcga tgcccgtgct ctcggtgacg cgtcgcgggt
      601 tgccgccgtt gcgatccatc aggtagacgt gcgagccgcc gtccttggac aacgtgacgg
      661 ccagcgtgcg gccgtccggc gagaacgccg gcgcgctgtt ggagcccttg aaggccgcga
      721 cctcgcgcat cgcgccggtg cgcacgttgc ggatcacgac cttggccttc ttgtcgtgga
      781 aggtcacgaa ggcgagctcg gagccgtcgg gcgaccaggc cggcgagatg atgggctcgt
      841 tgtccaccag cgccggcacg atgttggcgc cgtcggcgtc ggcgatgcgc agcgtgtgct
      901 tgccgccgcc cttggtcacg taggcgatgc gggtggagaa gacgccgcgc tcgccggtca
      961 ccttctggta gacgaggtcg gagatgtggt gggccgccag gcgcaggtcg gccggctgcg
     1021 tgggctcgct ctggccgccc tggtcggcgc ccttgacgac gtcccacagg cgaaagcgca
     1081 cgtcgacccg gccgtcggcc aggcgcgagg ccgaaccgcc caccacccag tcggcgccgc
     1141 gcgagcgcag ggtgttgtag tcgggcaggc cgttctcgtc ggccggggcc ggggcgtcga
     1201 cgaccttgaa gacgccgctg cgctcgaggt cggcgcgcac gacggccgag atggactggg
     1261 gctggcggtc ctcgtcgcgg aagcgtccga gcgcgatggg gatctgcgtg ccgccgacgc
     1321 cgcggatctg gacgaggaat tgcgcgaagg ccggcgtcat cgcgggcagc gcgagggagg
     1381 cgcccaaggc gcgcagggca tgtcttctag gccacatgag gattcaggtg agtcctgttc
     1441 agtcggaaaa gttccgggga aaccagcatg ctgttcagca ggcgcattgt aggcagcaag
     1501 aacgaggccc gcttgtaggc caacggcgca tccgcccggg gccggataat gcggggttca
     1561 cgccaccccc ggggaaccgc ggcctcagtc gcccgacccc gccgccaatg accacactga
     1621 agcagcgcct cgcgcgcgtg atcccctact tccgcgacaa cagccgcgcc gccctcgggc
     1681 tcgcggcgat ggcgatggtg gtggcggcgg ccacggagcc gatgatcccg tacatcctca
     1741 agcacctgtt gaacagcggc ttcaaggaca cgtcgggcct gcccctgtgg gccatcccgc
     1801 tggccatcgt ggtgctgttc atgatccgcg gcacgtcctg gtgggtgtcc agctacggcc
     1861 tgaccaaggc ctcgcagaac gcggtgctgg ctgtacgcgc gcacatgttc tcgcacatgc
     1921 tgcgcgccgc gcccgccctg ttcacgcgca acacggccag cagcatcacc aacaacatcg
     1981 tctacgagat ccagcagggc gcgaacctgc tgatgggctc ggtgcagatc ctgatccgcg
     2041 actcgctgac cgccctgttc ttcgtcggct acctggtcat cctcaactgg cgcctgacac
     2101 tgttcgtggt cgtgctgttc cccatcgtcg gcctgctcgt gcgcgtggtc agcaaacgcc
     2161 tgcacaagct caacgtcgcc ggccagaacg ccaccgacga cctcgcgtac gtcgtcgagg
     2221 agaacgtgct cgcctggcgc atcgtgcgcc tgcatggtgc ggccgcacag gaacagcgcc
     2281 gcttcgatgc gagttcgcac ctgatgcgca gcctcaacat caagcagcag gcgtcgggct
     2341 cgctgatgac gccggtcacg caggtgctgg cctcgttcgc gctcgccgcc gtgctgtgga
     2401 tggcgctggc cgagagcagc aagacgggcg ggacgccggg cgacttcgtg gccttcatca
     2461 cctgcatgct gctgctgatc cagccggcca agcacctgtc ggacgtgatg gccccgctca
     2521 cgcgcggcac ggccgcgatg gagcgcgggc tcgacatgat ggaggccgtc cccatcgagg
     2581 gcggcggcac gatcgccccg gcgcgcgtca agggcgacat cgagttccgc gacgtcgcgc
     2641 tgcgctacga cgcgcgcgac tcggtcgagc acgcgcagga cgacgcgccc gccgaggtgc
     2701 ccgccggcac gccggtggcc gcgctgtcgg acatcacgct ggccatccgc gccggcgaga
     2761 cggtcgcctt cgtcgggccg tcgggcgcgg gcaagacctc gctggtcaac ctgctgccgc
     2821 gcttcgtcga gcc
//
LOCUS       DATCRO010000362         7901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_678972, whole genome shotgun sequence.
ACCESSION   DATCRO010000362 DATCRO010000000
VERSION     DATCRO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678972"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VIN75_09945"
     CDS             complement(<1..1455)
                     /locus_tag="VIN75_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8974529.1"
                     /translation="MPLPKPPATRAALLSAADYESQSAPKTSRRSQKAAEPKQKLLDL
                     HDDRAETAVAKAAPRPAVAPAARAPATSPGLPVKSPAAYPIAPAPAARPKAAAGGARK
                     RARAGGPPKLFVLDTNVLLHDPTCLFRFEEHDVYLPMITLEELDNHKKGMTEVARNAR
                     QVSRDLDGLATEGSLDPVAGIPLSKTGHREAAGKLFFQTQFLDAKAPSGLPQGKADNQ
                     ILAVVQALREQHATREVVLVSKDINMRVKARALGLPAEDYFNDKTLDDADLLYTGVLA
                     LPADFWDKHGKTMESWNQGGHTFYRIVGPLVPSLLINQFVYLETPGEAPLYARVTEIT
                     GKSAVLKTLRDFTHAKNAVWGITARNREQNFALNLLMDPEVDFVTLTGTAGTGKTLAT
                     LACGLAQVMDDRRYSEIIVTRVTVPVGEDIGFLPGTEEEKMGPWMGALDDNLEVLSKG
                     DGGAGEWGRAATQDLVRSKIKVKSLNFMRGRTFLN"
     gene            complement(1708..3432)
                     /locus_tag="VIN75_09950"
     CDS             complement(1708..3432)
                     /locus_tag="VIN75_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974530.1"
                     /translation="MLSAAPIATSDTPDFGPNVHVFDASMPPAKIQAALDAAFEAQRL
                     SPTAQFGPQRHAFLFKPGRYDVVARVGYYTAVLGLGRSPDDVRITGNVLADSGWNVHD
                     KGNALINFWRSVENLAIAPAGGTTTWAVSQAAPMRRVHVRGRLRVSPTNDGQPGQGYS
                     SGGYLADSRVDGAVATGGQQQWYTRDSAVGGWSGKGWNTVFSGVRGAPPTTYPAPPVT
                     TLATTPASREKPYLYVDAKGRYRVFLPALRRDAAGPSWSAKAATPGTSRPLSRFFVVH
                     PGDSAATINAALAQGLDLLFTPGVYPVDQTIRVARAGTVVLGLGFPTLQPVGGVTPMA
                     VADVDGVRLGGLLFDAGTTNSDALLTIGAHGAHARHEADPISVQDVFLRIGGAVAGRA
                     TTGLVVDADDTLVDHVWAWRADHGDVKPGWTVNPADTGVAVDGDHVLATGLFVEHFRR
                     YQVVWNGEDGRTIFFQNEMPYDVPDQAGWTSGHGEGWAAYKVGADVKRHEAWGLGSYC
                     YFAIDHQNTHIVAARAFEVPETPGVRLHSLVTVSLGGNGTIRHIVNDSGPSTEPAGAT
                     AMTLRAWP"
     gene            complement(3677..5083)
                     /locus_tag="VIN75_09955"
     CDS             complement(3677..5083)
                     /locus_tag="VIN75_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY8974531.1"
                     /translation="MSMFPAATYEARRAALLGRLRGAGARGLVLLPGHVDSPMNYRDN
                     AYDFRQDSSFLYFCGLQQPELALVLDIDAGSATLHGDDIGIDHLVWTGPLPSVAERAA
                     QSGIAHAATSAQLASALASARAAGRDVHFLKPFRGETRLALSSWLALPDAQLDAAASR
                     ALTLAVIALRAVKSTSEIAEIENALHVTRDMHHLAMRLARPGVVEQEIVGAMEGLALT
                     HGRRLAYPSIFTSRGEILHNHDHSVRLKGGELIVNDTGANSPIGYASDITRTIPVGGK
                     FTGLQAELYDLVLESQTRAIAAVAPGVPYRDVHELACRVMVEGMKTLGFMRGDPAEAV
                     QAGAHAIFFQCGTGHMMGLDVHDMEGLGEDDVGYGQGYERSTLFGHKSLRLARPLQEG
                     FVVTIEPGVYINRWLTERWQAEGRHAGFIDYAMFDRHADFGGIRIEDDILVTADGHRE
                     LGPHIARSRADVEAACAA"
     gene            5285..7276
                     /locus_tag="VIN75_09960"
     CDS             5285..7276
                     /locus_tag="VIN75_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEY8974532.1"
                     /translation="MALHQLTDEQIKTWTRAQKDEWWLTTVYRGDMAQLTVRSAITGF
                     LLGGILAATAMYILAKTGIGIGVGLTSVLLSFAMFRAMSNAGIAKDFTVLENNCTQSI
                     ATSAGYVIAPLGAGLTAFMVMTGNVLSWWQMIVWLIVVSITGVLMAFPMKRRFINEEQ
                     MPFPEGRACGVVLDSLYSGGADVGLFKARLLYITAGITAVWELLVLDGWQRLIQFKIL
                     RMNVWAGLKEPWVLHDHLDDYYYAWLARIHEAAPRILGTDIRQLGLRFTLDFSMLGVG
                     GLMGVAVASSVMIGAILNLAVLAPIMIERGDIVARTLANGHVVAISRAEIVNQWSIWW
                     GVSMMVVGAMVGLFAKPDIFTKAFKSMSRAAGAPKERNDVLKHIEVPLWVSWIGVPAF
                     GLLGAWATHDFFGVPWLLSLVSLPLIYILTIICANSMGLTSWMPIGSLAKITQFSVGM
                     VDRANPASNLLPASMTSQISMNAASLLSDIKPGYMLGGKPRHQVWGHVIGIVSGAVFS
                     VPLFFLLFLRPDATGAVDATKIVSDAFPMPAAVQWRGVAEIISHGFHGAPVTAVWAAG
                     IAAVAAVVLEVLRIATKGRFPLSAVSIGLGVVLPFDSCVSMWIGAMGFWIMARLYDRK
                     PGSKGWTIWVDGAEPICAGLISAAALMGICDAILNVILG"
     gene            complement(7257..>7901)
                     /locus_tag="VIN75_09965"
     CDS             complement(7257..>7901)
                     /locus_tag="VIN75_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8974533.1"
                     /translation="DEVFADALGQRFAEQDDRVLREGREIVDQLELHLFPSRSPGWCL
                     THKLAWREPEPAGGRRGNTIVGLVGISRDLPEPGLSHATTAATYARVARVVERLQRHY
                     AEPLVLSELAREAGLSISQLGRQVLHLYRLTPRQLLARARLDAALQRLVDTTDSVADI
                     AHACGYADHSAFSRQFRRSTGVSPQQWRALQQQEQGRPAAALRLAGSRLSPGSR"
BASE COUNT         1090 a         2669 c         2880 g         1262 t
ORIGIN      
        1 gttcaggaag gtgcgcccgc gcatgaagtt caggctcttg accttgatct tcgagcgcac
       61 gagatcctgc gtggccgcgc ggccccattc gccggcgccg ccgtcgccct tggacagcac
      121 ctcgaggttg tcgtcgagcg cacccatcca cgggcccatc ttctcctcct cggtgccggg
      181 caggaagccg atgtcctcgc cgacgggcac cgtcacgcgc gtgacgatga tctcgctgta
      241 gcggcggtcg tccatcacct gcgccaggcc gcaggccagc gtcgccagcg tcttgccggt
      301 gccggcggtg ccggtgagcg tgacgaagtc gacctccgga tccatcagca ggttcagcgc
      361 gaagttctgt tcgcgattgc gcgcggtgat gccccagacg gcgttcttgg cgtgcgtgaa
      421 gtcgcgcagc gtcttgagca cggccgactt gccggtgatc tcggtgacgc gggcgtacag
      481 cggcgcctcg ccgggggtct cgaggtagac gaactggttg atgagcagcg acggcaccag
      541 cgggccgacg atgcggtaga acgtgtggcc gccctggttc cagctctcca tcgtcttgcc
      601 gtgcttgtcc cagaagtcgg cgggcagggc gagcacgccg gtgtagagca ggtcggcgtc
      661 gtcgagcgtc ttgtcgttga agtagtcctc ggccggcagg cccagcgcgc gcgccttgac
      721 gcgcatgttg atgtccttgg acaccagcac cacctcgcgc gtcgcgtgct gctcgcgcag
      781 cgcctgcacg acggccagga tctggttgtc ggccttgccc tggggcaggc cggacggcgc
      841 cttggcgtcg aggaactgcg tctggaagaa cagcttgccg gcggcctcgc ggtggcccgt
      901 cttggacagc gggatgccgg cgaccgggtc cagcgagccc tcggtggcca ggccgtcgag
      961 gtcgcgcgag acctggcgcg cgttgcgcgc gacttcggtc atgcccttct tgtggttgtc
     1021 gagctcctcc agcgtgatca tcgggaggta gacgtcgtgc tcctcgaagc ggaacaggca
     1081 cgtcgggtca tgcagcagca cgttggtgtc gagcacgaac agcttgggcg ggccgccggc
     1141 gcgtgcgcgc ttgcgggcgc cgcccgcggc ggccttgggc ctggcggccg gcgccggcgc
     1201 gatcgggtag gccgcaggcg acttcaccgg caggccgggc gacgtggccg gcgcgcgcgc
     1261 cgcgggggcg acggcggggc gcggggccgc cttggcgacg gccgtctccg cacggtcgtc
     1321 atggaggtcg agcaatttct gcttcggttc cgcggccttc tggctgcggc ggctcgtctt
     1381 gggagccgat tgcgattcgt agtcagcagc cgacagaaga gctgcgcgag tggcgggagg
     1441 cttcggaagt ggcatgtagg ggcgtacgag tcgcggaggt tggaaaatga acaaacaaaa
     1501 aagccgcgca gtcacctggg cggctttctt tgcttggatg tgcgcgttca aggtccacgg
     1561 gcatgcgaac catcatacac atttcgcgtg tcgtcaaaag cgatggtgct cggccgcaac
     1621 gccatgcctg ggtgcgcccg ggcggcctgc tggcggaggc gaatggcgcg actgcgcccg
     1681 gacgacggaa gaagtcccgc agggatgtca cggccacgcg cgcaaggtca tcgccgtcgc
     1741 tcccgccggc tccgtcgacg gcccgctgtc gttgacgatg tggcggatcg tgccgttgcc
     1801 gcccagcgag accgtgacga ggctgtgcag ccgcacgccg ggtgtctcgg gcacctcgaa
     1861 cgcacgcgcc gcgacgatgt gcgtgttctg gtggtcgatc gcgaagtagc agtagctgcc
     1921 caggccccag gcctcgtgcc gcttcacgtc cgcgcccacc ttgtaggcgg cccacccctc
     1981 gccgtggccg ctggtccagc cggcctggtc ggggacgtcg tagggcatct cgttctggaa
     2041 gaagatcgtg cggccgtcct cgccgttcca gaccacctgg taccggcgga agtgctcgac
     2101 gaacaggccc gtggccagca cgtggtcgcc gtcgacggcg acgccggtgt cggccgggtt
     2161 gaccgtccat cccggcttga cgtcgccgtg gtccgcgcgc caggcccaca cgtggtcgac
     2221 gagcgtgtcg tcggcgtcga ccaccaggcc tgtcgtggcc cggcccgcga ccgcgccgcc
     2281 gatgcgcaag aacacgtcct gcacgctgat cgggtcggcc tcgtggcggg catgcgcgcc
     2341 gtgcgcgccg atcgtgagca acgcgtcgct gttggtggtg ccggcatcga acagcaggcc
     2401 cccgaggcgc acgccgtcga cgtcggccac cgccatcggc gtgacgccgc ccacgggttg
     2461 cagcgtggga aatcccaggc cgaggacgac cgtgccggcg cgcgcgacgc ggatcgtctg
     2521 gtcgaccgga tagacgcccg gcgtgaacag caggtccagg ccttgggcga gcgccgcgtt
     2581 gatcgtggcg gcgctgtcgc cggggtggac gacgaagaag cggctcagcg gccgtgacgt
     2641 gcccggggtc gcggccttcg cgctccagct cgggcccgcg gcgtcgcggc gcagcgccgg
     2701 caggaagacg cggtagcggc ccttcgcgtc gacgtacagg tacggcttct cgcgcgacgc
     2761 cggcgtcgtc gcgagcgtgg tgaccggggg cgccgggtac gtcgtcggcg gcgctccgcg
     2821 cacgccggag aacacggtgt tccagccctt gcccgaccag ccgccgaccg cgctgtcccg
     2881 cgtgtaccac tgctgctggc cgcccgtggc gaccgcgccg tcgacgcggc tgtcggcgag
     2941 gtagccgccg ctggaatagc cctggcccgg ctggccgtcg ttggtcgggc tcacgcgcag
     3001 ccggccgcgc acgtgcacgc gccgcatcgg ggcggcctgg gacacggccc acgtcgtcgt
     3061 tccgcccgcg ggcgcgatcg ccaggttctc caccgagcgc cagaagttga tcagcgcatt
     3121 gcctttgtca tgaacgttcc agccgctgtc ggcgagcacg ttgccggtga tgcgcacgtc
     3181 gtcggggctg cgtcccaggc ccagcacggc ggtgtagtac cccacgcgcg cgacgacgtc
     3241 gtagcgcccc ggcttgaaca ggaaggcatg gcgctgcggg ccgaactgcg cggtggggct
     3301 caggcgctgc gcctcgaacg cggcgtcgag cgccgcctgg atcttggcgg gaggcatgga
     3361 ggcatcgaac acgtggacgt tcgggccgaa gtccggggtg tcggaggtgg cgatgggggc
     3421 ggcggaaagc atcgcgggca ggagcgcaag cagggcggtg cagctcgtca ggattcgggg
     3481 cgccatggat ctcggaagca gcgggggcgc ggcgattgtg gctgcgtatg aatgcgcttt
     3541 caagcctggc gaagccagag tggcctgcct gtcctcgtca ccgcggccag cgcatcgctg
     3601 gcgctcggac ccgcagcgcg atatcgagga gaggccagac ccctccggcg aggccagccc
     3661 cgggcgtgcc ggcgcctcag gccgcgcagg ccgcctcgac atccgcccgg ctgcgcgcga
     3721 tgtgcggccc cagctcgcgg tggccatcgg cggtgacgag gatgtcgtcc tcgatgcgga
     3781 tgccgccgaa atcggcgtgc ctgtcgaaca tcgcgtagtc gatgaacccg gcgtgccggc
     3841 cctcggcctg ccagcgctcg gtgagccagc gattgatgta gacgcccggc tcgatggtga
     3901 cgacgaagcc ctcctgcagc gggcgcgcca ggcgcagcga cttgtggccg aacagcgtgc
     3961 tgcgttcata cccctggccg taaccgacgt cgtcctcgcc gaggccttcc atatcgtgca
     4021 cgtccaggcc catcatgtgg ccggtgccgc actggaagaa gatcgcgtgc gcgccggcct
     4081 gcaccgcctc ggccgggtcg ccgcgcatga agcccagcgt cttcatgccc tcgaccatca
     4141 cgcggcaggc cagttcatgg acgtcgcggt agggcacgcc gggtgcgacg gcggcgatcg
     4201 cccgcgtctg cgactccagc accaggtcgt acagctcggc ctgcaggccc gtgaacttgc
     4261 cgccgaccgg gatcgtgcgc gtgatgtccg acgcatagcc gatgggcgag ttggcgccgg
     4321 tgtcgttgac gatcagctcg ccgcccttca ggcgcaccga gtggtcgtgg ttgtgcagga
     4381 tctcgccgcg gctcgtgaag atcgacgggt aggcgaggcg tcgcccgtgc gtcagcgcga
     4441 ggccttccat cgcgccgacg atctcctgct cgacgacgcc ggggcgcgcg aggcgcatcg
     4501 ccaggtggtg catgtcgcgc gtgacgtgca gcgcgttctc gatctcggcg atctccgacg
     4561 tcgacttgac cgcgcgcagc gcgatgacgg ccagcgtcag cgcgcggctg gccgcggcgt
     4621 cgagctgcgc gtcgggtagg gccagccacg aggagagcgc gaggcgcgtc tcgccgcgga
     4681 acggcttgag gaagtggacg tcgcggccgg ccgcgcgtgc gctcgcgagc gcggacgcca
     4741 gctgcgcgct ggtcgcggcg tgcgcgatgc cggactgcgc cgcacgctcg gcgacgcttg
     4801 gcagcggccc cgtccacacg aggtggtcga tgccgatgtc gtcgccatgc agcgtggcgc
     4861 tgccggcatc gatgtccagc accagcgcga gctcgggctg ctgcagcccg cagaagtaca
     4921 ggaagctcga gtcctggcgg aagtcgtacg cgttgtcgcg gtagttcatc gggctgtcga
     4981 cgtggcccgg cagaaggacg agtccgcggg cgccggcgcc gcgcaggcgg ccgagcaggg
     5041 cggcgcgacg cgcctcgtag gtggcggcgg ggaacatgga catggcggtc atctcgtgtt
     5101 tcggcagcga attgcgtgaa ttatcacgag gtctggccgc agaaagttcg gtcccgcgtg
     5161 gcctctaggc gttaactcgg tcgcagtcac gcgctggcgc tgcgagaatg cgcccccaaa
     5221 ggaaggaacc cggccacgag ccgcggcttc caagagacca ccgtttccgc caccgcctcc
     5281 acccatggcc ctgcaccaac tcaccgacga acagatcaag acctggaccc gcgcgcagaa
     5341 agacgagtgg tggctgacga ccgtgtaccg cggcgacatg gcgcaattga ccgtgcgttc
     5401 cgcgatcacc ggcttcctgc tcgggggcat cctcgccgcc acggcgatgt acatcctggc
     5461 caagacgggc atcggcatcg gcgtgggcct gacgtcggtg ctgctgtcgt tcgcgatgtt
     5521 ccgcgcgatg tccaacgccg gcatcgccaa ggacttcacc gtcctggaga acaactgcac
     5581 gcagtccatc gccacgtcgg ccggctacgt catcgcgccg ctgggcgcgg gcctgaccgc
     5641 cttcatggtg atgaccggca acgtgctgtc gtggtggcag atgatcgtct ggctgatcgt
     5701 cgtctcgatc acgggcgtgc tcatggcctt cccgatgaag cggcgcttca tcaacgagga
     5761 acagatgccg ttccccgaag ggcgcgcctg cggcgtggtg ctcgactcgc tgtacagcgg
     5821 cggcgccgac gtcggcctgt tcaaggccag gctgctgtac atcacggccg gcatcaccgc
     5881 cgtctgggaa ctgctggtgc tcgacggctg gcagcgcctg atccagttca agatcctgcg
     5941 catgaacgtg tgggccggcc tgaaggagcc ctgggtcctg cacgaccacc tggacgatta
     6001 ctactacgcc tggctcgcgc gcatccacga ggcggcgccc aggatcctgg gcaccgacat
     6061 ccgccagctg ggactgcgct tcacgctcga cttctcgatg ctgggcgtcg gcggcctgat
     6121 gggcgtggcg gtggcctcca gcgtgatgat cggcgccatc ctgaacctgg ccgtcctcgc
     6181 gcccatcatg atcgagcgcg gggacatcgt ggcgcgcacg ctggccaacg gccatgtggt
     6241 ggcgatctcg cgcgccgaga tcgtcaacca gtggtcgatc tggtggggcg tgtcgatgat
     6301 ggtggtcggc gcgatggtgg gcctgttcgc caagccggac atcttcacca aggccttcaa
     6361 gtcgatgtcg cgcgcggccg gcgcgcccaa ggagcgcaac gacgtgctca agcacatcga
     6421 ggtgccgctg tgggtgtcct ggatcggcgt gcccgcgttc ggcctcctcg gcgcgtgggc
     6481 cacgcacgac ttcttcggcg tgccctggct gctgtcgctg gtgtcgctgc cgctgatcta
     6541 catcctgacc atcatctgtg ccaactcgat gggcctgacg tcgtggatgc ccatcggctc
     6601 gctggccaag atcacgcagt tcagcgtggg catggtcgac cgcgccaacc cggccagcaa
     6661 cctgctgccg gcgtcgatga cctcgcagat ctcgatgaac gcggccagcc tgctgtcgga
     6721 catcaagcca ggctacatgc tgggcggcaa gccgcgccac caggtctggg gccacgtgat
     6781 cggcatcgtc tcgggcgccg tgttctcggt gccgctgttc ttcctgctgt tcctgcggcc
     6841 cgacgcgacg ggcgcggtcg acgccaccaa gatcgtctcc gacgcgttcc cgatgcccgc
     6901 ggcggtgcag tggcgcggcg tggccgagat catctcgcac ggcttccacg gcgcgccggt
     6961 gacggccgtg tgggcggccg gcatcgcggc ggtggcggcc gtcgtcctgg aagtgctgcg
     7021 tatcgccacg aagggccggt tcccgctgtc ggccgtctcc atcggcctgg gcgtggtgct
     7081 gccgttcgac tcctgcgtgt cgatgtggat cggcgcgatg ggcttctgga tcatggcgcg
     7141 cctctacgac aggaagcccg gcagcaaggg ctggacgatc tgggtcgacg gcgccgaacc
     7201 gatctgcgcg ggcctgatct cggccgcggc gctgatgggc atctgcgacg ccatcctcaa
     7261 cgtgatcctg ggctgaggcg actccccgcc aggcgaaggg cggccgcggg ccgcccttgt
     7321 tcttgctgct gcagggcgcg ccactgctgc gggctgaccc cggtgctgcg ccggaactgg
     7381 cgcgagaacg cgctgtggtc ggcgtagccg caggcgtgcg cgatgtcggc cacggagtcg
     7441 gtcgtgtcga ccaggcgttg cagcgcggcg tcgagccgcg cgcgcgcgag cagctggcgc
     7501 ggggtgaggc ggtacaggtg cagcacctgg cgcccgagct gcgagatcga cagccccgct
     7561 tcgcgcgcga gctccgagag cacgagcggc tcggcgtagt ggcgctgcag gcgctcgacc
     7621 acgcgtgcca cgcgcgcgta ggtcgccgcc gtcgtcgcgt ggctcaggcc cggctcgggc
     7681 aggtcgcgcg agatgccgac cagcccgacg atcgtgttgc cgcgccggcc gcccgcgggc
     7741 tcgggctcgc gccatgcgag cttgtgcgtg aggcaccagc ccggcgagcg gctggggaac
     7801 aggtgcagct cgagctggtc gacgatctcg cgcccctcgc gcaacacccg gtcgtcctgc
     7861 tcggcgaagc gctggccgag cgcgtcggcg aagacctcgt c
//
LOCUS       DATCRO010000363         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_847997, whole genome shotgun sequence.
ACCESSION   DATCRO010000363 DATCRO010000000
VERSION     DATCRO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847997"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="VIN75_09970"
     CDS             complement(<1..1723)
                     /locus_tag="VIN75_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974534.1"
                     /translation="MKNSTSNASSGARRAESHRQAGLLAHRARRWDDAVEEFERATEL
                     APQDALMWMNLARSRMAQGQPVPALEAARRACELDRGSAVACRMAAELALQMARPAEA
                     LDVLQALAPEAPRDHDYYNALGNALFQVRRPREAVDAYFLALAIQVDSAIVHYRLGLC
                     FMDLAMDFEAAQCFRTAISLDDGAIRALALSLVLQTNRQACAWEQDAQDTRALLEAVD
                     RDDPETARLLLPFTLISIDSTPQQQRRLNERRVAGLTSAIAPLPAPGPRKPGRIRVGW
                     LSSDFYHHATAVLMTELLERRDTGRFETFLYSHSRDDDTDTTRRVRAACEHFVDVRGL
                     GNCAVARRIRDDGIDILIDLKGHTRDSRMELMAYRPAPVQAAWLGYPATTGAPFIDYV
                     IGDRVVTPLEHAGHYTEHIAQLQGCYQPNDAARPLPPCPPRAAVGLPEDAVVLCCFNQ
                     TYKLSPRMLDLWARILAGAPRTVLWMLAWNPHARENLLRELAARGVAPERVFFAAKLE
                     LADHIARLRSADMFLDTWPCNAHTTASEALWAGVPVLTVPGATFASRVAASLVSACGL
                     GDLACRSE"
     gene            2000..>2173
                     /locus_tag="VIN75_09975"
     CDS             2000..>2173
                     /locus_tag="VIN75_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HEY8974535.1"
                     /translation="MTLSINTNIASIDAQRNLSASQSSLATSMQRLSSGLRINSAKDD
                     AAGLSIAERMNAQV"
BASE COUNT          318 a          747 c          800 g          308 t
ORIGIN      
        1 cctcgctgcg gcaggcgagg tcacccaggc cgcaggcgct gaccaggctc gcggccacgc
       61 gcgacgcgaa ggtcgcgccg ggcaccgtca gcaccggcac gcccgcccac agggcctcgc
      121 tggcggtggt gtgggcgttg cagggccagg tgtccaggaa catgtcggcc gagcgcaggc
      181 gcgcgatatg gtcggccagc tcgagcttgg cggcgaagaa gacgcgctcg ggcgcgacgc
      241 cgcgggccgc gagctcgcgc agcaggttct ccctggcgtg cgggttccag gccagcatcc
      301 acagcaccgt cctgggtgcg ccggccagga tgcgcgccca caggtccagc atgcgcggcg
      361 acagcttgta ggtctggttg aagcagcaca ggacgacggc gtcctcgggc aggccgacgg
      421 ccgcgcgcgg cgggcagggc ggcagcggac gggcggcgtc gttgggctgg tagcagccct
      481 gcagctgtgc gatgtgctcg gtgtagtggc cggcgtgctc cagcggcgtg acgacgcggt
      541 cgccgatcac gtagtcgatg aacggcgcgc ccgtcgtggc cgggtagccg agccaggcgg
      601 cctgcaccgg tgccgggcgg taggccatca gctccatgcg gctgtcgcgc gtgtggccct
      661 tcaggtcgat caggatgtcg atgccgtcgt cgcggatgcg acgggccacg gcgcagttgc
      721 ccaggccgcg cacgtcgacg aagtgctcgc acgccgcgcg gacgcggcgc gtcgtgtcgg
      781 tgtcgtcgtc gcggctgtgc gaatacagga aggtctcgaa acggcccgtg tcgcggcgct
      841 ccagcaattc ggtcatgagc accgcggtgg cgtggtggta gaagtccgag gacagccagc
      901 ccacccggat gcggccgggc ttgcgcggcc ccggcgcggg cagcggcgcg atcgcgctcg
      961 tcaggccggc gacgcggcgc tcgttgaggc ggcgctgctg ctgcggcgtc gagtcgatcg
     1021 agatcagcgt gaacggcagc agcaggcgcg cggtctcggg gtcgtcgcgg tcgacggcct
     1081 ccagcagcgc gcgcgtgtcc tgcgcatcct gctcccaggc gcaggcctgg cggttggtct
     1141 gcagcaccag gctcagcgca agggcgcgga tggcgccgtc gtccagcgag atcgcggtgc
     1201 ggaagcactg ggcggcctcg aagtccatcg ccaggtccat gaaacacagg cccaggcggt
     1261 agtggacgat ggccgagtcg acctggatgg ccagcgccag gaaataggcg tcgaccgcct
     1321 cgcgcgggcg gcgcacctgg aacagggcat tgcccagcgc gttgtaatag tcatggtcgc
     1381 gcggcgcctc gggcgccagc gcctgcagca cgtccagcgc ctcggccggc cgggccatct
     1441 gcagcgccag ctcggccgcc atccggcagg cgacggcgct gccgcggtcg agctcgcagg
     1501 cccggcgggc ggcctccagc gcgggcaccg gctggccctg ggccatgcgc gagcgcgcca
     1561 ggttcatcca catcagggcg tcctgcggcg ccagctccgt ggcgcgctcg aactcctcca
     1621 cggcgtcgtc ccagcggcgg gcgcggtgcg cgagcagccc ggcctggcgg tggctttcgg
     1681 cgcgacgggc gccggaagag gcgttcgatg tggagttctt catgatcggg ccagtctagg
     1741 ttcaagcccg ccggcaaggc ctccgataac agggccagtc acggtgcagt tcgcaggaac
     1801 gcccggggcg gccttcctgc cgggcaggga gcgcctgcca ccaggccgac ttcggcggtc
     1861 gtgaaaagaa ccgatcaaaa agtcctcaag ttcttcgatc ggccttccga aaaccagctc
     1921 acggccccta ccgtttttcg attttggggc gaacaacccg gcaatcccgc cggattttgt
     1981 ctcccctgga gtgaccatca tgactctctc gatcaacacc aacatcgcct ccatcgacgc
     2041 ccagcgcaac ctgtcggcgt cccagtcgtc gctcgcgacg tcgatgcagc gcctgtcctc
     2101 gggcctgcgc atcaactcgg ccaaggacga cgccgccggc ctgtcgatcg ccgaacgcat
     2161 gaacgcgcaa gtg
//
LOCUS       DATCRO010000364         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_679032, whole genome shotgun sequence.
ACCESSION   DATCRO010000364 DATCRO010000000
VERSION     DATCRO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679032"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2226)
                     /locus_tag="VIN75_09980"
     CDS             complement(163..2226)
                     /locus_tag="VIN75_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8974536.1"
                     /translation="MTPAMPRLTQAPATESLAALLAALAALGHAAWLVDAARGHIVAA
                     NVEAEELLERKDLEGLDAEDVINDLEDMAYWDGVRCGVVGILESDVELHRPGGAARPV
                     SRRIAPLWLADGTALFLVMMRDRSREHRVELERETALAELRATLEATADAILVTDLAG
                     RIRAFNRRFARMWSLPEAVLHAGDDRAVNEWVRMNVMGVDAYDRRLEEVFAHTLSEAV
                     DEIAQVNGAVIERHAQPQWSHGRPIGRVFSFRERNPYRTGAPREPGNLGVDQLTNLPN
                     RSGFVATLEETLRAGRESAGFAVLVIEFNRDALFGEGGDSAAGARRLSELAGQVRGAM
                     RQPHYCARLGGSRFGVLIDYASESAAESAARRLIEAQERSGGVPVAIGIGCWPGVGLS
                     ADEILRHVEIAMHRAQQATRSAFAVHKFGFDSWRRRRDRIEAGLLHASGEGRFRLAAQ
                     PRFDTKTGLAVAIEVHLRWRDRELGELPASQFMGVAEERGLVGALDDWVLEQGLLQLR
                     GWRAAGHALRLNINVSDWQLTQPGYARRVAAALDVAGCAPSELEIDVTESALQADPDA
                     ALAAVRSLARQGVRVLLEGFGDGTSSLAALRRLPLAGVKLDAVLSQAGARAGAEARWL
                     PALVQLAHAMDLEVHATGADTEAQCAALIAAGCDGLQGRRLGPWMEERALESWFSLPA
                     SRRRL"
     gene            complement(2223..3527)
                     /locus_tag="VIN75_09985"
     CDS             complement(2223..3527)
                     /locus_tag="VIN75_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8974537.1"
                     /translation="MTTPIHASALRIGMFVHLNVGWMSHPFPLSSFKLTSNEQIETIR
                     SLGLSRLTWSPERSDVEQPAVETAIAGAGTGAAASAAAAAEDEEAAPKPLDARQLARM
                     ALQAQLEIAQRCERQFTEACRDLRRTNDKVMTDPAAANAEAQMLARALLDKMLVEGEP
                     CLRVLGEMSSDRHASHALNVTVVSLMLGRQLNIPGNVLMELGVGALMHDVGKIALPDR
                     VRLPREDFNAGEAALYRDHVAQGLILGRRMGLSAEAQLVVAQHHELADGSGFPSALML
                     PRMSLPARIVALVNRYDNLCNAASPAQSITPHEALSRLFAQCRSKFDETLLSGFIKMM
                     GIYPPGSVVQLSDERFAMVMTVNAAHPLKPRVLVYDPSVPLEEAVHLDLEKTPQLGIR
                     RSLKPVQLPGRALDYLKPRQRISYFFDVESAPREDVAEEAAA"
     gene            3730..>4004
                     /locus_tag="VIN75_09990"
     CDS             3730..>4004
                     /locus_tag="VIN75_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY8974538.1"
                     /translation="MTDAFICDALRTPFGRYGGSLSAVRADDLGAIPIRALLARHPEL
                     DPAAIDDVLYGCANQAGEDNRNVARMSALLAGLPVDVPGVTLNRLCGS"
BASE COUNT          566 a         1473 c         1427 g          538 t
ORIGIN      
        1 ccgcccgccc aaccccgccc ttcggacatt cccacgccgc ccggcgtgtc gcccgcgccg
       61 gccaacgacc cgcatgggga ggatctcgat ctcgaggaag acgtcgcccc caacctgggc
      121 ggcgaggacc agatgcagga cgcctgagcg gcgcccggcc gctcagagcc ggcgacgcga
      181 ggccggcagg ctgaaccagg actcgagcgc gcgctcctcc atccacgggc cgaggcgccg
      241 tccctgcagg ccgtcgcagc ccgcggcgat cagcgcggcg cactgcgcct cggtgtcggc
      301 gccggtggcg tgcacttcga ggtccatcgc gtgcgccagc tgcaccagcg ccggcaacca
      361 gcgcgcctcg gcgccagcgc gggcgccggc ctgcgacagc acggcgtcca gcttgacgcc
      421 ggccagcggc agacggcgca gcgcggccaa cgagctggtg ccatcgccaa agccttcgag
      481 cagcacgcgc acgccctgcc gcgcgagcga gcgcacggcc gcaagcgcgg cgtcggggtc
      541 ggcctgcagc gccgactcgg tgacgtcgat ctcgagctcg gacggcgcgc agccggccac
      601 gtcgagcgcc gcggcgacgc ggcgcgcgta gcccggctgc gtcagctgcc agtcggacac
      661 gttgatgttc aggcgcagcg cgtggcccgc ggcgcgccag ccgcgcagct gcagcagccc
      721 ctgctccagc acccagtcgt cgagcgcgcc gaccaggccg cgctcctcgg cgacgcccat
      781 gaactggctg gccggcagct cgccgagctc gcggtcgcgc cagcgcaggt gcacctcgat
      841 ggcgacggcc aggcccgtct tcgtgtcgaa gcgcggttgc gcggcgagcc ggaagcggcc
      901 ctcgcccgag gcgtgcagca ggcccgcctc gatgcggtcg cgccggcggc gccacgagtc
      961 gaagccgaac ttgtgcaccg cgaacgccga gcgcgtcgcc tgctgcgcgc gatgcatcgc
     1021 gatctcgacg tggcgcagga tctcgtcggc cgacaggccc accccgggcc agcagccgat
     1081 gccgatggcc accggcaccc cgccggagcg ctcctgtgcc tcgatcagcc ggcgcgccgc
     1141 agactcggcg gcggactcgc tggcatagtc gatcagcacg ccgaagcggc tgccgcccag
     1201 gcgcgcgcag tagtgcggct ggcgcatcgc cccgcgcacc tggcccgcca gctcggacag
     1261 ccgccgggcg ccggcggcgc tgtcgccgcc ctcgccgaac agcgcgtcgc ggttgaattc
     1321 gatgacgagc acggcaaagc cggcgctctc gcggccggcg cgcagcgtct cttccagcgt
     1381 cgcgacgaat ccgctgcggt tgggcaggtt cgtcagctgg tcgacgccga ggttgcccgg
     1441 ctcgcgcggc gcgcctgtgc ggtacgggtt gcgttcgcgg aacgagaaca cgcgcccgat
     1501 cggccggccg tggctccact gcggctgcgc atggcgctcg atgacggcgc cgttgacctg
     1561 cgcgatctcg tcgaccgcct cggacagcgt gtgcgcgaag acctcctcca ggcggcggtc
     1621 gtaggcgtcg acgcccatca cgttcatgcg cacccactcg ttgaccgcgc ggtcgtcgcc
     1681 ggcgtgcagc accgcctcgg gcagcgacca catgcgggca aagcggcggt tgaacgcgcg
     1741 gatgcggccc gcgaggtcgg tcacgaggat ggcgtcggcc gtcgcctcga gcgtcgcgcg
     1801 cagctcggcc agcgcggtct cgcgctcgag ctcgacgcgg tgctcgcggc tgcggtcgcg
     1861 catcatcacg aggaacagcg cggtgccgtc ggccagccac agcggcgcga tgcggcgcga
     1921 gacggggcgc gcggccccgc cgggccggtg cagctcgacg tcgctctcca ggatgccgac
     1981 gacgccgcag cgcacgccgt cccagtaggc catgtcctcg aggtcgttga tcacgtcctc
     2041 ggcgtccagg ccctcgaggt ccttgcgctc gagcagctcc tcggcctcca cgttggcggc
     2101 gacgatgtgg ccgcgcgcgg cgtccaccag ccaggcggcg tggcccagcg cggcgagggc
     2161 cgccagcagg gcggcaagcg attcggtcgc cggcgcctgc gtcaggcgcg gcatcgcggg
     2221 cgtcatgccg cggcctcttc cgccacgtcc tccctcggcg cgctttccac gtcgaagaag
     2281 taggagatgc gctgccgcgg cttcaggtag tcgagcgcgc ggccgggcag ctgcaccggc
     2341 ttcagactgc ggcggatgcc cagctgcggc gtcttctcga ggtccaggtg caccgcctcc
     2401 tccagcggca ccgacgggtc gtagaccagc acgcgcggct tgagcggatg cgcggcgttg
     2461 acggtcatga ccatcgcgaa gcgctcgtcg ctgagctgca ccacggagcc cggcgggtag
     2521 atgcccatca tcttgatgaa gcccgacagc agcgtctcgt cgaacttcga tcggcactgc
     2581 gcgaacaggc gcgacagggc ctcgtgcggg gtgatggact gcgccggcga cgccgcgttg
     2641 cacaggttgt cgtagcggtt gaccagcgcc acgatgcgcg cgggcagcga catgcgcggc
     2701 agcatcagcg cgctcgggaa gccgctgccg tccgcgagct cgtggtgctg cgcgaccacc
     2761 agctgcgcct cggccgacag gcccatgcgc cggcccagga tcaggccctg ggcgacgtgg
     2821 tcgcggtaca gcgcggcctc gccggcgttg aaatcctcgc gcggcaggcg aacgcggtca
     2881 ggcagcgcga tcttgcccac gtcgtgcatc agcgcgccga cgcccagctc catgagcacg
     2941 ttgccgggaa tgttgagctg gcggcccagc atcagcgaga cgaccgtgac gttcagcgcg
     3001 tgcgacgcat ggcggtcgct ggacatctct cccagcacgc gcaggcaggg ctcgccctcg
     3061 accagcatct tgtcgagcaa cgcgcgcgcc agcatctgcg cctcggcgtt ggcggcggcc
     3121 gggtcggtca tcaccttgtc gttggtgcgg cgcaggtcgc ggcaggcctc ggtgaactgg
     3181 cgctcgcagc gctgggcaat ctccagctgc gcctgcagcg ccatgcgggc cagctggcgg
     3241 gcgtccagcg gcttgggagc ggcctcctcg tcctcggcgg ccgcggctgc ggaggcggcc
     3301 gcgccggtgc cggcgcctgc gatggccgtc tcgacggcgg gctgctcgac gtcgctgcgc
     3361 tccgggctcc acgtcagccg agacaggccc agagagcgga tcgtctcgat ctgctcgttg
     3421 gaggtcagct tgaaactcga caacgggaac ggatgcgaca tccagccgac gttcagatga
     3481 acgaacatgc cgatgcgcag ggcgctcgcg tggatggggg tggtcatcga aaggttcctt
     3541 ggctgcccgt gagggcccgg gcagcttctt cctccgtgaa atcggccgga tgaccgtccg
     3601 acttgagcgg acggatcaac ggatgaaccg gatgaagccg gaaagcgcgc aaaatccgcc
     3661 ggcaggaccc ggattccgaa taattgttct tcctgcgaac aaatctgcca gaagagacgc
     3721 ccacccacca tgaccgacgc cttcatctgc gatgcactgc gcacgccatt cggccgctac
     3781 ggcggttccc tgtccgcggt gcgagccgat gacctgggtg ccattccaat ccgggcgctg
     3841 ctggcgcgcc atcccgaact cgaccccgcg gcgatcgacg acgtcctgta cgggtgcgcc
     3901 aaccaggccg gcgaggacaa ccgcaacgtc gcccgcatga gcgccctgct ggccggcctg
     3961 ccggtcgacg tgcccggcgt cacgctgaac cgcctgtgcg gctc
//
LOCUS       DATCRO010000365         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_60306, whole genome shotgun sequence.
ACCESSION   DATCRO010000365 DATCRO010000000
VERSION     DATCRO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60306"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VIN75_09995"
     CDS             complement(<1..1416)
                     /locus_tag="VIN75_09995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8974539.1"
                     /translation="MTPANPVVAFARRLADGWWRSGMLAVYTDEDRDSAQIRAAHLGA
                     VIRLTPFTMAANFLNGALVVWAYRADRPTGMWAWLGALYALCLMALAGWWRGRHRVST
                     MATPAAIRHANVHANLPALVWALLPILWFAHAQPQQQILVVALVTGMLAAGAFVLSPL
                     PVAATCFVAIFASAGVVACWLTGDRIFVAVGCLQTIYAAVVVLGTLAMSRKATALLKS
                     QAEAKRQERMMAVLLHDFEQNAAEALWETGRDGCVAHHSPRLATLLGLDEAMLRQRPF
                     LELLAHGGVDVAPLRAALEAERPFKELRLSASAPHGARHWQINGKPLDDDEARIVGWR
                     GVIADVTEQVMAQERLQHLAHTDSLTGLANRFTLHGALRAAIAQRRLVALLALDLDHF
                     KLVNDTHGHAAGDQLLQHVAARLLACRRPADLVARLGGDEFAVLVLDDPGAAVDVDAL
                     SQRLIDVLEEPVDIGGRATRAS"
     gene            1687..>3066
                     /locus_tag="VIN75_10000"
     CDS             1687..>3066
                     /locus_tag="VIN75_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974540.1"
                     /translation="MTNASGGARQPPRRLVPIGRSLLASSLAVAVLSLALAFAGVQSL
                     LVEPARRAVARAELERSTREVDAALSSLFKRVDTIARLRRDWGALGLMSNTDEAGIVR
                     LLGTELMHEPAVSSVTIADDSGREVLLRKAGPGQFRTRSTDPQAHPGTEVDATWTLDG
                     RLVERTTAASDYDARRRPWFVQVHETPPGPEVVWTEPFTFRSTGRPGLSAVVQWKTPD
                     GRVNSSTTDVTLDDISRFTRGFEVSAHGFAVVLTDDGRLVGLPGGAAAVDDARAAALA
                     LQPIAALGQPALAAAFDDWRAQGSPPGTALRLGVGGQAWLAHFSRIAIRGRASLHVAV
                     LAPESDFALVGTRQWATLAAILLVTLLLTAAAALSVARRFARPLRQLSEESERIGRLD
                     LARPVQVASPWREVADTAAAQESMRQDLLRATEGLEAAVERRTEQLTVAVEAAGEASR
                     AKAAFLANMS"
BASE COUNT          396 a         1122 c         1131 g          417 t
ORIGIN      
        1 ggatgcgcgc gtcgcgcggc cgccgatgtc cacgggttcc tccagcacgt cgatcaggcg
       61 ctgcgacagc gcgtcgacgt cgaccgcggc gccggggtcg tccagcacca gcacggcgaa
      121 ttcgtcgccg cccaggcgcg cgaccaggtc ggcgggccgg cggcacgcga gcaggcgcgc
      181 ggccacgtgc tgcagcagtt ggtcgccggc ggcgtggccg tgggtgtcgt tgaccagctt
      241 gaagtggtcg aggtccaggg ccagcagcgc gaccagccgc cgctgcgcga tcgccgcgcg
      301 cagcgcgccg tgcagcgtga agcggttggc caggccggtc agcgagtcgg tgtgggcgag
      361 gtgctgcagg cgttcctgcg ccatcacctg ttcggtgacg tcggcgatca cgccgcgcca
      421 gccgacgatg cgcgcctcgt cgtcgtccag cggcttgccg ttgatctgcc aatgccgggc
      481 gccatgcggc gcgctggccg acagccgcag ctccttgaac ggccgctccg cctcgagcgc
      541 ggcgcgcagc ggcgcgacgt ccacgccgcc gtgggccagc agttccagga atggacgctg
      601 gcgcagcatc gcctcgtcca gccccagcag cgtggccagg cgcggcgagt ggtgcgccac
      661 gcagccgtcc cgccccgtct cccacagcgc ctcggcggcg ttctgctcga agtcgtgcag
      721 cagcacggcc atcatgcgtt cctggcgctt ggcctcggcc tgcgacttga gcagcgccgt
      781 ggccttgcgc gacatcgcca gcgtgcccag cacgacgacc gccgcgtaga tcgtctgcag
      841 gcagcccacc gccacgaaga tgcggtcgcc ggtgagccag cacgcgacga cgcccgcgga
      901 cgcgaagatc gccacgaagc acgtcgccgc caccggcagc gggctcagca cgaaggcgcc
      961 ggccgccagc atgcccgtga cgagcgccac caccaggatc tgctgctgcg gctgcgcatg
     1021 cgcgaaccag aggatgggca ggagcgccca caccagcgcc ggcaggttgg cgtgcacgtt
     1081 ggcatggcgg atcgccgccg gcgtggccat cgtgctcacg cggtggcggc cgcgccacca
     1141 gccggccagc gccatcaggc acagcgcgta cagcgccccc agccaggccc acatgccggt
     1201 gggccggtcg gcgcggtagg cccagaccac cagcgcgcca ttgaggaagt tggccgccat
     1261 cgtgaacggc gtgagccgga tcacggcgcc caggtgcgcg gcgcggatct gggcggaatc
     1321 gcgatcctcg tcggtgtaca cggcgagcat gcccgagcgc caccagccgt cggcgagacg
     1381 gcgggcgaag gcgacgaccg ggttcgctgg cgtcattggc gcgttgtgga ttcttttcgt
     1441 tggcgcggcg cgaccctcgc ggcgcgcctg cggtcgagca ggtgttcccg tgggttatcg
     1501 gcaggccgat ggcgaaatcg atgagcgatt ctgtgccttt gtgcctcgcg cgtcgcgcaa
     1561 gtcctgaaat taggggacgg acagccgggt ttgggggaca gggacgtgga ctagactcga
     1621 caatcggctg cggcgcttgt cgtggcccgc gccgtcgttg gcgtcggcgc cgatctggga
     1681 ggatggatga cgaacgcttc cggaggcgcc aggcagccgc cgcgccgcct cgtgcccatc
     1741 ggcaggagcc tgctggcgtc ctcgctggcc gtggccgtgc tgtcgctggc gctggcgttc
     1801 gccggcgtcc agagcctcct ggtcgaaccg gcgcgccgcg ccgtcgcccg ggccgagctc
     1861 gagcgctcca cccgcgaggt cgacgccgcc ctgtccagcc tgttcaagcg cgtcgacacg
     1921 atcgcccggc tgcggcgcga ctggggcgcg ctggggctga tgtccaacac cgacgaggcc
     1981 ggcatcgtcc gcctgctcgg caccgaactg atgcacgagc cggccgtgtc gtcggtgacg
     2041 atcgccgacg attccggccg cgaggtgctg ctgcgcaagg ccggccccgg ccagttccgg
     2101 acgcgctcga ccgatcccca ggcgcacccc ggcaccgagg tcgacgccac ctggacgctg
     2161 gacggccggc tcgtcgagcg cacgaccgcc gccagcgact acgacgcccg ccggcgcccg
     2221 tggttcgtcc aggtgcatga gacgccgccc gggccggagg tggtctggac cgagccgttc
     2281 actttccgtt cgaccggacg gcccggcctg tcggccgtcg tgcaatggaa gacgcccgac
     2341 ggccgtgtca attccagcac caccgacgtc accctcgacg acatctcccg cttcacgcgc
     2401 ggcttcgagg tcagcgcgca tggctttgcc gtggtgctga ccgacgacgg ccggctcgtc
     2461 ggcctgccgg gcggtgccgc cgcggtcgac gatgcgcgcg ccgccgcgct ggcgctgcag
     2521 ccgatcgccg cgctcggcca gcctgccctg gcggctgcgt tcgacgactg gcgcgcgcag
     2581 gggtcgccgc cgggcaccgc gctgcgcctg ggcgtgggcg ggcaggcctg gctggcgcat
     2641 ttctcgcgca tcgcgatccg gggacgcgct tcgctgcacg tggcggtgct ggcgcccgaa
     2701 tcggacttcg ccctggtcgg cacgcgccaa tgggcgacgc tggccgcgat cctgctggtc
     2761 accttgctgc tgaccgccgc cgcggcgctg agcgtggcgc gccgcttcgc gcgcccgctg
     2821 cggcagctgt ccgaggagag cgagcgcatc ggccggctcg acctcgcgcg cccggtgcag
     2881 gtggcgagcc cgtggcggga agtggccgac accgccgccg cccaggaatc gatgcgccag
     2941 gacctgctgc gcgccaccga aggcctggag gcggccgtcg agcggcgcac cgagcagttg
     3001 accgtcgccg tcgaggcggc cggcgaggcc tcgcgcgcca aggcggcctt cctggccaac
     3061 atgagc
//
LOCUS       DATCRO010000366         7926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_352502, whole genome shotgun sequence.
ACCESSION   DATCRO010000366 DATCRO010000000
VERSION     DATCRO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352502"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VIN75_10005"
     CDS             <1..935
                     /locus_tag="VIN75_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8974541.1"
                     /translation="KPADLVPGSVWALCEILSRCGLPAGVLNLVMGRGAQVGATMLED
                     RGIDAISFTGSVATGQRVAQACVARMAKFQLEMGGKNPMVVLDDADLGVAINCAVQSG
                     FYSTGQRCTASSRVIVTEGIHDRFVAGVIDKLRTLKVDNARKPGTDIGPVVDATQLKQ
                     DLDYVGIAQAEGAKLAFGGEAIERNADGQSGFYMRPALITESAPQMRINREEVFGPVV
                     SVSRVRDYEEALAQANDTPFGLSAGIATTSLKHATHFKRHAQAGMVMVNVPTAGVDYH
                     VPFGGRKGSSYGPREQGRYAAEFFTVVKTAYTQA"
     gene            1234..3402
                     /locus_tag="VIN75_10010"
     CDS             1234..3402
                     /locus_tag="VIN75_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY8974542.1"
                     /translation="MASATRSHSVLLTPLAAAALALAGPARADDTPVQSVQVTARSAS
                     DTAGVTGWGQPLSRTPIQADVIDSKVLADIGATSLSDLTRIDASITDSYNAVGYWTTF
                     TVRGFQVDNQRNFRRDGLPINAETSLPLDNKSDVEVLKGLSGMQSGTSAPGGIVNLVV
                     KRPDADLRSATLLWSQTGTVGAAADLSQRFGERRQFGARVNLSATRLDPNFRDDKGHR
                     SLASIATDWRIADGSLLEFEFEHSVQSQPSVPAFSTWGGVVPDPHQIDPHINLNDQPW
                     SIPVVLQGNTMSLRFTQALAPDWTLKVQALQQRLVSNDHLAFAFGGNADCVPCNSFAS
                     DGSFALYDFRSDDERRQTRDLDISVAGTFTTGPLRHAATAGALETVFTGRFNDEAYNF
                     VGIGTSDGQTMIPVSQDDPSLTTPNTDRTERSTEIYLRDAVELGADWSAWGGLRHTRL
                     HRQTAPTSTGDASTATDYRQSFTTPWLAVSRQLGEHDMVYVSWGEGAESSVTPNAPFF
                     KDKGLPEPLTRSRQWEGGFKHAIGADQWGIAVYDVVQPQYADVLADPSQSASDTNEYL
                     HVRDGNKHTRGIEGEAQTRIDGLMLRASVMAQRARNEGGSDPAIGRTPTNVPGRSVKL
                     LADYAVAALPGLSVLANMAYEGPREVFPDDSTRIPGWTRWDLGARYVQSLGGTTLTWR
                     AGVDNLFDRRAWREAPYQYDHAYLFPLEPRTFHASLEAKF"
     gene            3442..3517
                     /locus_tag="VIN75_10015"
     tRNA            3442..3517
                     /locus_tag="VIN75_10015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3475..3477,aa:Asn,seq:gtt)
     gene            complement(3619..4011)
                     /locus_tag="VIN75_10020"
     CDS             complement(3619..4011)
                     /locus_tag="VIN75_10020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8974543.1"
                     /translation="MSNTEIERLSGSGVLPATLPFPEGTAVGPLVFLSGQLGNVPGTL
                     TLVPGGIRAEARQALENIATVLRRQGLDIANLVRCTVMLADMAEWPAFNEAYAEFFAG
                     HPLPARSAFGCNGLALGARVEIECTAAR"
     gene            complement(4071..6068)
                     /locus_tag="VIN75_10025"
     CDS             complement(4071..6068)
                     /locus_tag="VIN75_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8974544.1"
                     /translation="MTGIHRRGPAAFALLLLIVGGTPALAHDDDVRIHGVAVGTQALK
                     ALPGGVTQADYGFSERGRGDQIHARWTLDARGLPATYDAQGNDYWKVPITEHFEVAAG
                     RASWNNRIETGSAEWAAPGAFYLPANAPPEFTGVLARALLKAPGHRLKLLPAGEAWLE
                     RGARVDAGGHALTLYRLGGIEFTPVPVWLDEQGATAAVIDDWFEVLSATAKPRFAKLQ
                     AAQAAADQAWSADVARRLAHRPEGALMIRHARLFDPRDMAVRDDIRVLVRGGHVVRVD
                     PDDTGAAPPDTEVIDAAGRFLMPGLWDVHQHFSGVDGAFDLVAGVTSARDMANDNVPL
                     MARVARFDAGTELGPRVVLAGIVEGTGPLAGPTPVRIENLKQAQDAVDWYAAHGYEQI
                     KIYSSFPPALVRPIADMAHARGLRVSGHVPAFMRAREFIEAGADEIQHMNFIVLNFFP
                     DVQDTRSKDRYTITADKLQQLRLDSPEFADFLRLLQARHTVLDPTLSVFQDLYSGAPG
                     RCAHSLCPFIERFPPVTRRRLLSGAVAVPEGKDEVYAQALPALERLLKRLHDGGVTLM
                     AGTDAFAGYTLHNELALYVEAGIPAPEVLRLATLVPAQVLGVAGPSQRGFIAPGKQAD
                     MILVDGDPTQDIGDLRHVWRTIKDGKVYDPAQIEQAMGVSR"
     gene            complement(6079..7167)
                     /locus_tag="VIN75_10030"
     CDS             complement(6079..7167)
                     /locus_tag="VIN75_10030"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY8974545.1"
                     /translation="MIPSRLEPRYATINALGVLDNPNLPLGPAEAGDPALVSGTRAID
                     ARALADARSAGLSAVNITLGHVAGSGDAFAETVRDITGWNAFIARHAAMLRRVQCVSD
                     ISAATQAGQVGVIYGFQNTEMLGREIDRVRLFAKMGVRVIQLTYNGRNAVGDGATVQG
                     DAGLTRFGAEVVEKLQAENVLVDLSHSSEKTCLDALAVAKRPLAITHSACRAVADHPR
                     NKSDAELRRLADQGGVLGLYFMPYLRLAGQPMAADLVAHLEHALHVCGEDHVGLGTDG
                     GTTLHDDMTAYRRRLEDEIAQRRALGIGAPGETADVVTFLPDLCGPTQFQRLADLLAA
                     RGHGASRIEKILGGNFRRLMAQAWSLPH"
     gene            7280..>7926
                     /locus_tag="VIN75_10035"
     CDS             7280..>7926
                     /locus_tag="VIN75_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8974546.1"
                     /translation="MARKNLSAAQAAAAERLLAHALPDARHASRRSVLRHALTLFNAN
                     GVEATTIEHLRKASGQSVGTIYHHFKNKEGVVAALFFVALDDQSRAIASRIEGLHDGR
                     AVVEALIEATTEWITAQPECAYFVFLARDLVAQGPYGDDLTQRLLARYSPIDEILARD
                     AEAGRILPLPGDLIPALVLGATEFYARSWLAGRRQAAPTTHAQRLATAAWRSLTAG"
BASE COUNT         1183 a         2875 c         2757 g         1111 t
ORIGIN      
        1 tcaagccggc cgacctggtg ccgggctcgg tgtgggcgct gtgcgagatc ctgtcgcgct
       61 gcggcctgcc cgccggcgtg ctcaacctcg tcatgggccg cggcgcgcag gtgggcgcca
      121 cgatgctgga ggatcgcggc atcgacgcga tcagcttcac cggctcggtg gccacgggcc
      181 agcgcgtcgc gcaggcgtgc gtcgcgcgca tggccaagtt ccagctcgag atgggcggca
      241 agaatccgat ggtcgtgctc gacgacgccg acctgggcgt cgcgatcaac tgcgcggtgc
      301 agagcggctt ctactccacc ggccagcgct gcacggcgtc atcgcgcgtg atcgtcaccg
      361 agggcatcca tgaccgcttc gtcgcgggcg tcatcgacaa gctgaggacg ctcaaggtcg
      421 acaacgcgcg caagccgggc accgacatcg gcccggtggt cgacgcgacg cagctcaagc
      481 aggatctgga ctacgtgggc atcgcgcagg ccgagggcgc gaaactcgcg ttcggcggcg
      541 aggcgatcga gcgcaacgcc gacggccagt ccggcttcta catgcgtccc gcgctgatca
      601 ccgagtcggc gccgcagatg cgcatcaacc gcgaggaagt gttcggcccg gtggtcagcg
      661 tgagccgcgt gcgcgactac gaggaagcgc tggcgcaggc caacgacacc ccgttcgggc
      721 tgtcggccgg catcgccacc acgtcgctga agcacgccac gcatttcaag cgccacgcgc
      781 aggccggcat ggtgatggtc aacgtgccca ccgcgggcgt ggactaccac gtgcccttcg
      841 gcggccgcaa gggatcgagc tacgggccgc gcgaacaggg ccgctacgcg gcggagttct
      901 tcaccgtcgt gaagacggcg tacacgcagg cctgaggcct ccggcgcgcg ggtcccgagc
      961 agcctgcgcg caacacccgc cccgcgtttt cccggggttt ggggcacaat cccacccgcg
     1021 cgtcgcaatc ctgcggcgcg catttcgcta ggggtgctgg ccgagaggct ggctgagaaa
     1081 gtccctttga acctgattgg ccttcacggc caggccgacg gcgcaagccg cggtccgagg
     1141 taatcctcgc gcagggaagc ggtcgcaccg acgggaccat tcgcgtcccg gtcttccgac
     1201 tccgttcccc tgccatcgct ttgaaagagc tcgatggctt ccgctacccg ttcccattcc
     1261 gttctcctga cgccgctcgc cgcggccgcg ctcgcgctcg ccggcccggc gcgcgcggac
     1321 gacacgcccg tgcagagcgt gcaggtcacc gcgcgcagcg cgtccgacac cgccggcgtc
     1381 accggctggg gccagccgct ctcgcgcacg ccgatccagg ccgacgtcat cgacagcaag
     1441 gtgctggccg acatcggcgc cacctcgctg tcggacctca cgcgcatcga cgccagcatc
     1501 accgactcgt acaacgcggt cggctactgg acgaccttca ccgtgcgcgg cttccaggtc
     1561 gacaaccagc gcaacttccg ccgcgacgga ctgcccatca acgcggagac ctcgctgccg
     1621 ctggacaaca agtcggacgt cgaggtgctc aagggcctga gcggcatgca gtcgggcacg
     1681 agcgcgccgg gcggcatcgt caacctggtg gtcaagcgcc cggacgccga cctgcgcagc
     1741 gccacgctgc tgtggtcgca gacaggcacc gtcggcgccg cggccgacct gagccagcgc
     1801 ttcggcgagc gccgccagtt cggcgcccgc gtgaacctgt cggccacgcg cctcgatccg
     1861 aacttccgcg acgacaaggg ccatcgcagc ctcgcgtcga tcgcgaccga ctggcgcatc
     1921 gccgacggca gcctgctcga attcgagttc gagcacagcg tgcagtcgca gcccagcgtg
     1981 ccggcgttca gcacctgggg cggcgtggtg cccgacccgc accagatcga cccgcacatc
     2041 aacctgaacg accagccgtg gtcgatcccg gtcgtgctcc agggcaacac gatgtcgctg
     2101 cgcttcacgc aggcgctcgc gccggactgg acgctcaagg tgcaggcgct gcagcaacgc
     2161 ctggtcagca acgaccacct ggccttcgcc ttcggcggca acgcggactg cgtcccctgc
     2221 aacagcttcg ccagcgacgg cagcttcgcg ctctacgact tccgcagcga cgacgagcgc
     2281 cgccagacgc gcgatctcga catctcggtc gccggcacgt tcacgaccgg cccgctgcgc
     2341 cacgcggcca ccgccggcgc gctcgagacg gtgttcaccg gccgcttcaa cgacgaggcc
     2401 tacaacttcg tgggcatcgg caccagcgac ggccagacga tgatcccggt cagccaggac
     2461 gacccgtccc tgacgacgcc caacaccgac cgcaccgaac gcagcaccga gatctacctg
     2521 cgcgacgcgg tcgaactcgg cgccgactgg tccgcctggg gcggactgcg ccacacgcgc
     2581 ctgcatcgcc agacggcgcc gacgtccacg ggcgacgcca gcacggccac cgactaccga
     2641 cagtcgttca cgacgccgtg gctggccgtc agccgccagc tgggcgagca cgacatggtc
     2701 tacgtgagct ggggcgaagg cgccgagagc agcgtcacgc ccaacgcccc gttcttcaag
     2761 gacaagggcc tgccggagcc gctgacgcgc agccgccagt gggaaggcgg cttcaagcat
     2821 gcgatcggcg cggaccagtg gggcatcgcg gtctacgatg tcgtgcagcc gcagtacgcc
     2881 gacgtgctgg ccgacccgtc gcagtccgcc agcgacacca acgagtacct gcacgtgcgc
     2941 gacggcaaca agcacacgcg cggcatcgag ggcgaggcgc agacgcgcat cgacgggctg
     3001 atgctgcgcg ccagcgtgat ggcgcagcgc gcgcgcaacg agggcggcag cgacccggcc
     3061 atcggccgca cgccgaccaa cgtcccgggg cgttccgtga agctgctggc cgactacgcc
     3121 gtcgccgccc tgcccggcct gagcgtgctg gccaacatgg cctacgaggg cccgcgcgag
     3181 gttttccccg acgactcgac gcgcatcccc ggctggacgc gctgggacct cggcgcccgc
     3241 tacgtgcagt cgctgggcgg caccacgctc acgtggcgcg ccggcgtcga caacctgttc
     3301 gaccgccgcg cctggcgcga ggcgccttac cagtacgacc acgcctatct gttcccgctc
     3361 gagccgcgta ccttccacgc atcactcgaa gcaaagtttt gacgttctcg aaaatctggt
     3421 tatactgaga ggcttcgcag ttccccgata gctcagtcgg tagagcgccg gactgttaat
     3481 ccgtaggtcc ctggttcgag cccaggtcgg ggagccagcc tcgcgacgac gtcgcggcaa
     3541 gaagtgctca tgaagcccat ccggtcgcgg ttgggctttt tgctttccgg atcgttcgcg
     3601 cagccggccc gtcgtccttc aacgtgccgc cgtgcactcg atctcgaccc gcgcgcccag
     3661 cgccaggccg ttgcagccga acgcgctgcg cgccggcagc ggatgacccg cgaaaaactc
     3721 ggcgtaggct tcgttgaagg ccggccactc ggccatgtcg gccagcatca cggtgcagcg
     3781 cacgaggttg gcgatgtcca ggccctgcct gcgcagcacc gtcgcgatgt tctccagcgc
     3841 ctggcgggcc tcggcgcgga tgccgcccgg caccagcgtg agcgtgccgg gcacgttgcc
     3901 caactggccc gacaggaaca ccagcggccc gaccgcggtg ccctccggga acggcagcgt
     3961 ggccggcagc acgccgctgc cggacaggcg ttcgatctcc gtgttgctca caaaagcttc
     4021 cctccgcctg acggctgcag tgcgagcgac ttggggcggc cgtgcgtcgc tcatcgactc
     4081 acccccatcg cctgctcgat ctgcgccgga tcgtagacct tgccgtcctt gatcgtgcgc
     4141 cagacgtggc gcaggtcgcc gatgtcctgg gtgggatcgc cgtcgaccag gatcatgtcg
     4201 gcctgcttgc ccggcgcgat gaagccgcgc tgcgacggcc ccgccacgcc cagcacctgc
     4261 gcgggcacca gcgtggccag gcgcagcacc tcgggcgcgg ggatgccggc ctcgacgtac
     4321 agcgccagct cgttgtgcag cgtgtagccg gcgaacgcgt cggtgccggc catcagcgtg
     4381 acgccgccgt cgtgcaggcg cttgagcagg cgttcgagcg ccggcagcgc ctgcgcgtag
     4441 acctcgtcct tgccctcggg caccgcgacc gcgccggaga gcaggcggcg ccgcgtcacc
     4501 ggcgggaagc gctcgatgaa cgggcacagg ctgtgtgcgc agcggccggg cgcgccggag
     4561 tacaagtcct ggaacaccga gagcgtcgga tccagcacgg tgtggcgggc ctgcagcagc
     4621 cgcaggaaat cggcgaactc tggcgagtcg agccggagct gctgcagctt gtccgccgtg
     4681 atcgtgtagc gatccttgct gcgcgtgtcc tgcacgtcgg ggaagaagtt cagcacgatg
     4741 aaattcatgt gctggatctc gtcggcgccg gcctcgatga actcccgcgc gcgcatgaac
     4801 gcgggcacat gcccgctcac gcgcagcccg cgcgcgtggg ccatgtcggc gatgggacgc
     4861 accagcgcgg gcgggaacga cgagtagatc ttgatctgct cgtagccgtg ggcggcgtac
     4921 cagtcgacgg cgtcctgcgc ctgcttcagg ttctcgatgc gcaccggcgt cggacccgcg
     4981 agcggaccgg tgccctcgac gatgccggcg agcacgacgc gcgggcccag ctccgtgccg
     5041 gcgtcgaagc gcgcgacgcg ggccatgagc ggcacgttgt cgttggccat gtcgcgcgcg
     5101 ctggtgacgc cggcgacgag atcgaacgcg ccgtccacgc cggagaaatg ctggtgcacg
     5161 tcccacaggc ccggcatcag gaatcggccc gcggcgtcga tcacctcggt gtcgggcggc
     5221 gcggcgccag tgtcgtcggg gtcgacccgc acgacgtggc cgccacgcac cagcacgcgg
     5281 atgtcgtcgc gcaccgccat gtcgcgcggg tcgaacaggc gcgcgtggcg gatcatcagc
     5341 gcgccctcgg gccggtgcgc gaggcggcgc gcgacgtccg cgctccaggc ctggtcggcc
     5401 gcggcctgcg cggcctgcag cttcgcgaac cgcggcttcg cggtggccga cagcacctcg
     5461 aaccagtcgt cgatgacggc cgcggtggcg ccctgctcgt ccagccagac cggcaccggc
     5521 gtgaactcga tcccgcccag gcggtacagc gtcagcgcgt ggccgccggc gtcgacgcgc
     5581 gcgccgcgct cgagccaggc ctcgcccgcg ggcagcagct tcagccgatg gccgggcgcc
     5641 ttgagcagcg cgcgcgccag cacgcccgtg aactcgggcg gcgcgttggc cggcaggtag
     5701 aacgcacccg gggccgccca ctcggccgag cccgtctcga tgcggttgtt ccagctcgcc
     5761 cggccggcgg cgacctcgaa gtgctcggtg atcggcacct tccagtagtc gttgccctgg
     5821 gcgtcgtagg tggcgggcag gccgcgcgcg tcgagcgtcc agcgcgcatg gatctggtcg
     5881 ccgcgcccgc gttcgctgaa gccgtagtcg gcctgcgtca cgccgccggg caaggccttg
     5941 agcgcctggg tgccgaccgc cacgccatgg atgcggacat cgtcgtcgtg cgcgagcgcc
     6001 ggcgtgccgc cgacgatcag caacagaagg gcgaacgcgg ccgggccgcg acggtggatg
     6061 ccggtcatgc ggggcctttc agtgcgggag gctccaggct tgcgccatca ggcggcggaa
     6121 gttgccgccc aggatcttct cgatgcgcga cgcgccatgg ccgcgcgcgg ccagcaggtc
     6181 ggccaggcgc tggaactggg tcgggccgca caggtcgggc aggaaggtga cgacgtccgc
     6241 cgtctcgccc ggcgccccga tgcccagcgc gcggcgctgc gcgatctcgt cctcgagccg
     6301 gcgacggtac gccgtcatgt cgtcgtgcag cgtggtgccg ccgtcggtgc ccaggcccac
     6361 gtggtcctcg ccgcagacgt gcagcgcgtg ctccaggtgc gcgacgaggt cggcggccat
     6421 cggctggccg gcgaggcgca ggtagggcat gaagtacagg cccagcacgc cgccctggtc
     6481 ggccagccgg cgcagctcgg cgtcggactt gttgcgcggg tggtcggcca ccgcgcggca
     6541 ggccgagtgc gtgatcgcca gcgggcgctt ggcgaccgcg agcgcgtcca ggcacgtctt
     6601 ctcgctgctg tggctcaggt cgacgagcac gttttcggcc tgcagcttct cgaccacctc
     6661 cgcgccgaag cgcgtgaggc cggcatcgcc ctgcaccgtc gcgccgtcgc ccaccgcgtt
     6721 gcggccgttg tacgtgagtt ggatgacgcg cacgcccatc ttggcgaaca gccgcacgcg
     6781 gtcgatctcg cgtcccagca tctcggtgtt ctggaagccg tagatgacgc cgacctggcc
     6841 ggcctgggtg gcggcgctga tatcggagac gcactgcacg cggcgcagca tcgccgcgtg
     6901 tcgggcgatg aacgcgttcc agccggtgat gtcgcgcacc gtctcggcga acgcgtcgcc
     6961 gctgccggcc acatgcccga gcgtgatgtt gaccgccgac agcccggcgc tgcgggcgtc
     7021 ggccagcgcg cgtgcgtcga tggcgcgcgt gccgctgacc agcgccgggt cgcccgcctc
     7081 cgccggcccc aggggcaggt tcgggttgtc cagcacgccc agggcgttga tggtcgcgta
     7141 gcggggctcg agtcgggaag gaatcacggc gtcctcgggg ttgtgccggg aactggcggc
     7201 acggcggaat ggatgcggtt ccagcaaggc cgaaattcta gaacactatt ctagaacatt
     7261 gttcgaatag aatgcagcca tggcacgcaa gaacctgtcg gccgcccagg cggccgccgc
     7321 cgaacggctg ctggcgcacg ccctgcccga cgcgcgccac gccagccgcc gcagcgtgct
     7381 gcgccacgcg ttgacgctgt tcaacgccaa cggcgtcgag gccaccacca tcgaacatct
     7441 gcgcaaggcc agcggccaga gcgtgggcac gatctaccac cacttcaaga acaaggaagg
     7501 cgtcgtcgcg gcgctgttct tcgtggcgct cgacgaccag agccgcgcca tcgccagccg
     7561 catcgagggc ctgcacgacg gccgcgcggt ggtcgaggcg ctgatcgagg ccaccacgga
     7621 atggatcacc gcgcagcccg agtgcgcgta cttcgtgttc ctcgcgcgcg acctggtcgc
     7681 ccagggcccg tacggcgacg acctgacgca acgcctgctc gcgcgctact cgccgatcga
     7741 cgagatcctg gcccgcgacg ccgaggccgg ccgcatcctg ccgctgcccg gcgacctcat
     7801 ccccgcgctg gtgctggggg ccaccgagtt ctacgcgcgc agttggctgg ccgggcgacg
     7861 ccaggccgcg ccgacgacgc atgcgcagcg cctcgccacg gcggcctggc gctctttgac
     7921 ggcggg
//
LOCUS       DATCRO010000367         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_195150, whole genome shotgun sequence.
ACCESSION   DATCRO010000367 DATCRO010000000
VERSION     DATCRO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195150"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VIN75_10040"
     CDS             <1..970
                     /locus_tag="VIN75_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974547.1"
                     /translation="QIQKGEFIVFVGPSGCGKSTLLRLIAGLEPITSGNLLLDGKDIT
                     WAPSGKRDLAMVFQSYALYPHMSVYDNMSFALKLAGVSKSEIKTKVEYAAKTLNLTQY
                     LDRTPKDLSGGQRQRVAIGRAIVRAPKVFLFDEPLSNLDAALRGNTRVEIHKLHRALG
                     ATTIYVTHDQVEAMTLADRVVVLRDGLIEQVGSPLELYDRPANQFVAQFIGMPSMNMV
                     AANAIPTFSAATGGRLPADGFLGVRPEGLRVHPKQGAPVSDVLGRVELVEALGADTLI
                     HVDVGGVPLIARQNDRTPLQAGDDVAVELDPSVLHLFNREGRSVFA"
     gene            1053..2456
                     /gene="dalD"
                     /locus_tag="VIN75_10045"
     CDS             1053..2456
                     /gene="dalD"
                     /locus_tag="VIN75_10045"
                     /EC_number="1.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
                     /protein_id="HEY8974548.1"
                     /translation="MLHLGLGSFHRAHQAVYLQRLIDAGDTRWSISGANIRPDMAEVV
                     AALEAQGGRYTLETVSPAAEYRYEEISAIREVLPWEPSLAKVIARGAAPSTRIVSFTV
                     TEAGYYLDDKGRLDLSFPDLAADVERARRGESGETEGKGVTIYGAVCAILRARKATNA
                     GPLTLLNCDNLRHNGDRFRAGLLEFIELAGDADLLAWVKANTRCPNAMVDRITPRPPP
                     ELRARVKAATGRDDAAAITGESFIQWVIEDDFAAGRPDWGRVGVQLVESVQPYEEAKI
                     RILNATHSCVAWAGTLVGLGFIHEGTHDAAIRKMAYDYVTDDVIPCLTPSPIDLAAYR
                     DVVLDRFGNPAIRDTNQRVAADGFSKIPGFIAPTVRERLAAGQPIESVAMLPALFLAF
                     LQRWHKGALPYVYQDQGMDEAVAHAICDAADPVVALCSDVGLWGDIAGDQRLIDAVRR
                     ANERVARFVQAASGHRP"
     gene            2453..>2540
                     /locus_tag="VIN75_10050"
     CDS             2453..>2540
                     /locus_tag="VIN75_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HEY8974549.1"
                     /translation="MSGRLQGRHVLLTGAGGGIGLAVAAACIG"
BASE COUNT          391 a          902 c          869 g          378 t
ORIGIN      
        1 gcagatccag aagggcgagt tcatcgtctt cgtcgggccc tcgggctgcg gcaagtcgac
       61 gctgctgcgg ctgatcgcgg ggctggagcc gatcaccagc ggcaacctgc tgctggacgg
      121 caaggacatc acctgggcgc catcgggcaa gcgcgatctc gcgatggtgt tccagagcta
      181 tgcgctctat ccgcacatga gcgtgtacga caacatgtcc ttcgcgctca agctggcggg
      241 cgtctccaag agcgagatca agaccaaggt cgagtacgcg gcgaagacgc tgaacctcac
      301 gcagtacctc gaccgcacgc ccaaggacct ctcgggcggc cagcgccagc gggtggccat
      361 cgggcgcgcc atcgtgcgtg cgccgaaggt gttcctgttc gacgagccgc tgtcgaacct
      421 cgacgccgcg ctgcgcggca acacgcgcgt ggagatccac aaactgcacc gcgcgctcgg
      481 cgcgaccacc atctacgtga cgcacgacca ggtcgaggcc atgacgctgg ctgaccgcgt
      541 ggtggtgctg agggacgggc tgatcgagca ggtcggctcg ccgctggagc tgtacgaccg
      601 cccggccaac cagttcgtcg cgcagttcat cggcatgccg tcgatgaaca tggtggcggc
      661 aaacgcgatt cccaccttct ccgccgccac cggaggcagg ctgcccgccg acggcttcct
      721 gggcgtgcgc cccgaggggc tgcgcgtgca tccgaagcag ggcgcgccgg tgtcggacgt
      781 gctcggccgc gtcgagctgg tggaggcact gggcgccgac acgctgatcc acgtcgacgt
      841 gggcggggtg ccgctgatcg cgcgtcagaa cgaccgcacg ccgctgcagg ccggcgacga
      901 cgtggcagtg gagctcgatc cgtcggtgct ccatctcttc aaccgcgagg gccgttcggt
      961 cttcgcgtga catcgaattt ccgtccaccg tcttttcaac ggaccttctc gtgaccattg
     1021 cgcaaactcc accggctcct tccgagttca cggtgctcca tctcggcctg ggctcgttcc
     1081 accgcgccca ccaggccgtg tacctgcagc ggctgatcga cgcgggcgac acccgctggt
     1141 cgatctccgg cgccaacatc cggccggaca tggccgaggt cgtcgcagcg ctcgaggcgc
     1201 agggcgggcg ctacacgctc gaaaccgtct cccccgcggc cgagtaccgc tacgaggaga
     1261 tctccgcgat tcgcgaggtg ctgccctggg agccctcgct ggccaaggtg atcgcgcgcg
     1321 gcgcggcgcc ctcgacgcgc atcgtgtcct tcaccgtgac cgaggcgggc tactacctcg
     1381 acgacaaggg caggctcgac ctgtcgttcc ccgacctcgc ggccgacgtg gagcgcgcgc
     1441 gccgcggcga aagcggcgag accgaaggca agggcgtgac gatctacggc gccgtctgcg
     1501 ccatcctgcg cgcgcgcaag gccacgaacg ccggcccgct gacgctgctg aactgcgaca
     1561 acctgcgcca caacggcgac cgcttccgcg ccggcctgct ggaattcatc gaactggcgg
     1621 gcgatgccga tctgctcgcc tgggtgaagg cgaacacgcg ctgcccgaac gcgatggtcg
     1681 accgcatcac gccgcgcccg ccgcccgaac tgcgcgcccg cgtgaaggct gccacgggtc
     1741 gcgacgacgc ggccgccatc accggcgaga gcttcatcca gtgggtgatc gaggacgact
     1801 tcgcggccgg ccggcccgac tggggccgcg tgggcgtgca gctggtcgag tcggtgcagc
     1861 cttatgaaga ggccaagatc cgcatcctca acgccacgca cagctgcgtg gcctgggcgg
     1921 gcacgctggt ggggctcggc ttcatccacg agggcacgca cgacgcggcc atccgcaaga
     1981 tggcctacga ctacgtcacc gacgacgtca ttccctgcct gactcccagc cccatcgacc
     2041 tcgcggcgta tcgcgacgtg gtgctcgacc gcttcggcaa ccccgccatc cgcgacacca
     2101 accagcgcgt ggcggcggac ggcttctcga agatccctgg cttcatcgcg ccgacggtgc
     2161 gcgagcgcct cgcggccggc cagcccatcg agagcgtggc gatgctgccc gcgctgttcc
     2221 tggccttcct gcagcgctgg cacaagggcg cgctgcccta cgtctaccag gaccagggca
     2281 tggacgaggc cgtggcgcac gcgatctgcg acgctgcaga tcctgtggtc gcactgtgtt
     2341 ccgacgtggg gctgtggggc gacatcgcgg gcgaccagcg cctgatcgac gccgtgcgtc
     2401 gcgcgaacga acgcgtcgcg cgcttcgtgc aggccgcttc ggggcacagg ccatgagcgg
     2461 ccggctgcag ggcaggcacg tgctgctgac cggcgctggc ggcggcatcg ggctggccgt
     2521 ggccgcggcc tgcatcggcg
//
LOCUS       DATCRO010000368         5994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_453901, whole genome shotgun sequence.
ACCESSION   DATCRO010000368 DATCRO010000000
VERSION     DATCRO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453901"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="VIN75_10055"
     CDS             complement(<1..2643)
                     /locus_tag="VIN75_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8974550.1"
                     /translation="MGRPARSTPAVAARDGFVRVRGAREHNLKDVEVAIPRDALVVFT
                     GVSGSGKSSLAFGTIYAEAQRRYFESVAPYARRLIDQVGVPDVDEIAGLPPAVALQQA
                     RGTPGARSSVGSVSQLSSLVRMLYSRAGTYPPRQTMLYAEDFSPNTAQGACPHCHGLG
                     RVFEVTEQTMVPDPSLSIRERAIASWPPAWHGQNLRDILVTLGYDVDRPWRDLPKKDR
                     DWILFTQEQPTVPVYAGFTPAETRAALKSKTEPSYMGTFMGARKYVLHTFATTQSALM
                     KKRVSRYMVGGLCPVCEGKRLKREALSVTFAGVDIGTMGQMPLDGVAALLEPAAAGRF
                     GDPAPARDAQAPTRAAAKADIARRVAAGGSAHAASPDVRRTPNLSEEKRLAAQRIAQD
                     LCARIATLRGLGLGYLSMERSTPTLSPGELQRLRLATQIRSNLFGVVYVLDEPTAGLH
                     PADGEALLDALEQLKAQGNSLFVVEHDLEVMRRADWLVDVGPDAGEKGGRVLYSGPPD
                     GLREVAASHTAHHLFAKAASRPRAPRTPSHWLELRGVTRNNLRGLDARIPLGLFTAVT
                     GVSGSGKSSLVSQALVELVGAHLGHEPEADDDESDDAAPEVVQATRGRLGAGAEAIRR
                     LVRVDQKPIGRTPRSNLATYTGLFDAVRKLYADTPAARKRRYDAGRFSFNVAKGRCQT
                     CEGEGFVSVELLFMPSVYAPCPACHGARYNADTLKITWNGRHIAQVLDMTVDDALAFF
                     DGESAVRKPLSVLSDIGLGYLRLGQPATELSGGEAQRIKLATELQRPQRGDTLYVLDE
                     PSTGLHPSDVDRLMAQLQGLVDAGNTVVVVEHEMRLVAASDWVIDMGPGAGDEGGRIV
                     ACGPPDEVARAGASRTAPYLRAVLG"
     gene            complement(2649..3524)
                     /locus_tag="VIN75_10060"
     CDS             complement(2649..3524)
                     /locus_tag="VIN75_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8974551.1"
                     /translation="MTDPDRNYPKPPFPEQQQSWPALACRMQPPPDHGETSYVGSNRL
                     AGRKALITGGDSGIGRAVAIAFAREGADVAINYLPDEEEDARAVVALIEQAGRKAVPL
                     PGDLRDAAFCRRLVEDAAKGLGGLDIVVNNAGRQASFDSIVDIPDEHFDDVFKTNVYA
                     LFHVTKAAVKHLRRGAAIINTSSVQAFDPSKDLLDYAATKAAILSMTRSLAKQLGEQG
                     IRVNAVAPGPYWTPLQLAGGQPMDKLVKFGEKTPMGRPGQPAEIAGQFVLLASQESTF
                     TTGNVWGSTGGETGP"
     gene            complement(3661..>5994)
                     /locus_tag="VIN75_10065"
     CDS             complement(3661..>5994)
                     /locus_tag="VIN75_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974552.1"
                     /translation="PPTSAEQVAMTLQAFSADVQVTAQGPTTTLTSGGVATYTMTVSN
                     AGPDASSNLTLQDNVDASQTLGQVTCVASGGATCPATLGGAMTVPTLPAGGTLVFTVP
                     STIAANALGRVSNTMYATPSGDPVAANNAATATASTAIGANGAASFVTLQSDAGDYIG
                     AGQSYAYSNANAKLTVTASGGHVQLAIDGNDSWSADFAEPSALATLQAGSYQNLTRYP
                     FDDATVGGLSWWGNGRGCNTLTGNFTIDRATYSGSTLTGIDLHFEQHCEGAAPALRGQ
                     IHWLASDTSTAPGPVNPVPSTLWRAPAAATPASGNYVYLQSDQNDYIGAGATQVYTQA
                     NAVLSPTLSGLHLGIAVTGNDNWTGDFQGMTSISQLQPGYYANLQRYPFNNPVTGGLS
                     WSGDGRGCNTLTGWFAIDSITVTAGALTAVDLRFEQHCEGGAAALHGQIHWVAGDTTA
                     APGPQNPPPANLWAPAAGATPAAGNYVYLQSDQGDYIGGGATLTYTQANAVLSVTSNG
                     KHLGVSVTGNDGWGGDFQAMDSVAQLQPGYYPGLQRYPFNNPVTGGLSWSGDGRGCNT
                     LTGWFVVDSITYTAGVLTAVDLRFEQHCEGGTAALHGKIHWAAGDTTTPPGPQTPPAG
                     LWAPPTGATPASGNFVYLQSDPGDYIGGGATELDTAANATISVYGSGALANVSVGGWT
                     GNFRGMNSIAQLQPGYYANLQRYPFNNPVTGGLSWYGNGRGCNTLTGWFVVDGVTYDA
                     NGLSSLDLRFEQHCEGGTAALHGQVHWTNGASSGGTH"
BASE COUNT          762 a         2113 c         2215 g          904 t
ORIGIN      
        1 gcccagcacc gcacgcagat agggcgcggt gcggctcgcg cctgcgcgtg cgacctcgtc
       61 cggcggaccg cacgcgacga tgcgcccgcc ctcgtcgccc gcgccggggc ccatgtcgat
      121 cacccagtcg ctggcagcga cgaggcgcat ctcgtgctcg acgaccacca ccgtgttgcc
      181 cgcatccacc aggccctgca gctgcgccat cagccggtcg acgtccgacg ggtgcagccc
      241 cgtgctgggt tcgtccagca cgtacagcgt gtcgccgcgc tgcggccgct gcagctccgt
      301 cgcgagcttg atgcgctgcg cctcgccacc cgacagctcg gtggccggct gcccgagccg
      361 caggtagccc aggccgatgt cgctcagcac ggacagcggc ttgcgcaccg cggactcgcc
      421 gtcgaagaag gccagcgcgt cgtcgacggt catgtccagc acctgggcga tgtggcggcc
      481 gttccacgtg atcttcagcg tgtccgcgtt gtagcgcgcg ccgtggcagg ccgggcacgg
      541 cgcgtagacg ctgggcatga acagcagctc gacgctgacg aagccctcgc cctcgcaggt
      601 ctggcagcgc cccttggcga cgttgaacga gaaccggccc gcgtcgtagc gccgcttgcg
      661 cgccgcgggc gtgtcagcgt agagcttgcg caccgcgtcg aacaggccgg tgtacgtcgc
      721 gaggttggag cgcggcgtgc ggccgatggg cttctggtcg acgcgcacga ggcggcggat
      781 cgcctcggcg cccgccccca ggcggccacg cgtcgcctgc acgacctcgg gcgccgcgtc
      841 gtcggactcg tcgtcgtcag cctccggttc gtggccgaga tgcgcgccga ccagctcgac
      901 cagcgcctgg ctcacgagac tggacttgcc cgagcccgac acgcccgtga ccgccgtgaa
      961 caagcccagc gggatgcgcg cgtcgaggcc gcgcaggttg ttgcgcgtca cgccgcgcag
     1021 ctccagccag tgcgagggcg ttcgaggcgc gcgcgggcgc gacgcggcct tggcgaacag
     1081 gtggtgcgcc gtgtgcgagg ccgcaacctc gcgcaggcca tcgggtggcc cgctgtacag
     1141 cacgcggccg cctttctcgc cggcgtccgg gccgacgtcg acgagccagt cggcgcgccg
     1201 catcacctcc aggtcgtgct cgacgacgaa cagcgagttg ccttgcgcct tcagctgctc
     1261 gagcgcgtcg agcagcgcct cgccgtcggc cggatgcagc cccgcggtgg gctcgtccag
     1321 cacgtagacg acgccgaaca ggttggagcg gatctgcgtg gccaggcgca ggcgctgcag
     1381 ttcgccgggc gacagcgtgg gcgtgctgcg ctccatcgag agataaccca ggcccaggcc
     1441 ccgcagcgtg gcgatgcgtg cgcacaggtc ctgcgcgatg cgctgcgcgg ccagccgctt
     1501 ctcctccgac aggttgggcg tgcgtcgcac gtccggcgac gccgcgtggg ccgagccccc
     1561 ggccgcgacg cggcgcgcga tgtcggcctt ggccgccgcg cgcgtgggcg cctgcgcatc
     1621 gcgcgcgggc gcggggtcgc cgaagcggcc ggcggcggcc ggctcgagca gcgcggcgac
     1681 gccgtcgagc ggcatctgcc ccatcgtgcc gatgtccacg cccgcgaacg tcaccgacag
     1741 cgcctcgcgc ttgagccgct tgccttcgca gacggggcac aggccgccca ccatgtagcg
     1801 cgacacgcgc ttcttcatca gcgcgctctg cgtcgtcgcg aacgtatgca gcacgtactt
     1861 gcgcgcgccc atgaacgtgc ccatgtagct gggttccgtc ttcgacttga gtgccgccct
     1921 ggtctcggcg ggcgtgaagc cggcgtagac gggcacggtg ggctgctcct gcgtgaacag
     1981 gatccagtcg cgatccttct tcggcaggtc gcgccacggg cggtcgacgt catagcccag
     2041 cgtcacgagg atgtcgcgca ggttctgtcc gtgccaggcc ggcggccatg acgcgatggc
     2101 gcgctcgcgg atcgacagcg acgggtcggg caccatcgtc tgctcggtga cctcgaacac
     2161 gcggcccagg ccgtggcagt gcgggcaggc gccctgggcc gtgttgggcg agaagtcctc
     2221 cgcatagagc atcgtctgcc gcggcgggta ggtgccggca cgcgaataca gcatgcgcac
     2281 gaggctggac agctgcgaga cgctgcccac cgacgagcgc gcgcccggcg tgccgcgcgc
     2341 ctgctgcagc gcgacggccg gcggcaggcc ggcgatctcg tcgacatcgg gcacgcccac
     2401 ctggtcgatc aggcgcctcg catacggcgc gacggactcg aagtagcgcc gctgggcctc
     2461 ggcgtagatc gtgccgaacg ccagcgagga cttgcccgac cccgacacgc cggtgaacac
     2521 gaccagcgcg tcgcgcggga tcgcgacctc gacgtccttc aggttgtgct cgcgcgcgcc
     2581 gcgcacgcgc acgaagccgt cgcgcgcggc gaccgcgggg gtggagcgcg ccggccggcc
     2641 catgcccgtc aggggcccgt ctccccgccc gtgctgcccc acacgttgcc ggtggtgaag
     2701 gtcgactcct gcgacgccag cagcacgaac tgcccggcga tctcggccgg ctggccgggc
     2761 cggcccatcg gagtcttctc gccgaacttc acgagcttgt ccatcggctg gccgccggcc
     2821 agctgcagcg gcgtccagta gggcccgggc gcgaccgcgt tgacgcggat gccctgctcg
     2881 cccagctgct tggccagcga gcgcgtcatc gacaggatgg ccgccttggt cgccgcgtag
     2941 tccagcaggt ccttcgacgg atcgaaggcc tgcaccgacg aggtgttgat gatggccgcg
     3001 ccgcgcctga ggtgcttcac cgcggccttg gtgacgtgga acagcgcgta gacgttggtc
     3061 ttgaagacgt cgtcgaagtg ctcgtccggg atgtcgacga tcgagtcgaa cgaggcctgc
     3121 cggccggcgt tgttgacgac gatgtccagg ccgcccaggc ccttggcggc gtcttccacg
     3181 agccggcggc agaaggccgc gtcgcgcagg tcgcccggca gcgggaccgc cttgcggccg
     3241 gcctgctcga tgagcgcgac gacggccctg gcgtcctcct cctcgtcggg caggtagttg
     3301 atcgcgacgt cggcgccttc gcgcgcgaac gcgatcgcca ccgcgcggcc gatgcccgaa
     3361 tcgccgccgg tgatcaacgc cttgcggccg gccaggcggt tcgagccgac gtagctcgtc
     3421 tcgccatggt cgggcggcgg ctgcatccgg cacgcgagcg cgggccacga ttgctgctgt
     3481 tcgggaaagg gcggcttggg atagttcctg tccgggtcgg tcatggcgtc ctcgcgtttc
     3541 ggtcgaacgc ttgaacggca agacgcatac cccgccggcc agggacgcgc gggcggacat
     3601 cgcgccgcgg cggtccgccg gcgtcgacgc ggcgcgggtc cggcccgcgc cgccgcgcgc
     3661 tcagtgcgtg ccgccgctcg acgcgccgtt cgtccagtgc acctggccgt gcagcgccgc
     3721 cgtgccgcct tcgcaatgct gctcgaagcg caggtccagc gaggacaggc cgttggcgtc
     3781 ataggtgacg ccgtcgacga cgaaccagcc ggtcagcgtg ttgcagccgc ggccgttgcc
     3841 gtaccaggac agcccgccgg tgacggggtt gttgaacgga tagcgctgca ggttcgcgta
     3901 gtagccgggc tgcagctgcg cgatgctgtt catgccccgg aagttgcccg tccagccacc
     3961 gaccgagacg ttcgccagcg cgcccgagcc gtagaccgag atcgtggcat tggccgcggt
     4021 gtcgagctcg gtcgcgccgc cgccgatgta gtcgcccgga tcgctctgca ggtagacgaa
     4081 gttgcccgag gccggcgtgg cgcccgtcgg cggcgcccac aggcccgcgg gcggcgtctg
     4141 cggcccgggc ggcgtggtgg tgtcgccggc ggcccagtgg atcttgccgt gcagcgctgc
     4201 cgtaccgcct tcgcaatgct gctcgaagcg caggtcgacg gctgtcagga cgcccgccgt
     4261 gtaggtgatg ctgtcgacca cgaaccagcc ggtgagcgtg ttgcagccgc ggccgtcgcc
     4321 cgaccagctc aggccgccgg tgaccgggtt gttgaacgga tagcgctgca gtcccgggta
     4381 gtagcccggc tggagttgcg cgacgctgtc catggcctgg aagtcgccgc cccagccgtc
     4441 attgccggtg accgagacgc ccaggtgctt gccgttggac gtcaccgaca gcacggcgtt
     4501 ggcctgcgtg taggtcagcg tggcgccacc gccgatgtag tcgccctggt cgctctgcag
     4561 gtagacgtag ttgcccgcgg ccggcgtggc gccggcggcc ggcgcccaca ggttggcggg
     4621 cggcgggttc tgcgggccgg gcgcagcggt ggtgtcgccg gcgacccagt ggatctggcc
     4681 gtgcagcgcg gcggcgccgc cctcgcagtg ctgctcgaag cgcaggtcca ccgccgtcag
     4741 cgcgccggcg gtcacggtga tgctgtcgat cgcgaaccat cccgtgagcg tgttgcagcc
     4801 gcggccgtcg ccggaccagc tcaggccgcc ggtgaccggg ttgttgaacg gatagcgctg
     4861 caggttcgca tagtagccgg gctgcagctg cgagatgctg gtcatgccct ggaagtcgcc
     4921 cgtccagttg tcgttgcccg tgacggcgat gcccaggtgc aggcccgaca gcgtggggct
     4981 gagcaccgcg ttggcctgcg tgtagacctg cgtggcgccg gcgccgatgt agtcgttctg
     5041 gtcgctctgc aggtagacgt agttgcccga ggccggcgtg gcggccgccg gggcgcgcca
     5101 cagcgtcgac ggcaccggat tgaccgggcc gggcgccgtg gacgtgtcgc tggcgagcca
     5161 gtggatctgg ccgcgcagcg cgggagccgc gccctcgcag tgctgttcga agtgcaggtc
     5221 gatcccggtg agcgtcgagc ccgagtacgt cgcccggtcg atcgtgaagt tgccggtcag
     5281 cgtgttgcag ccccggccgt tgccccacca gctcaggccg ccgaccgtgg cgtcgtcgaa
     5341 cggatagcgc gtcaggttct ggtagctgcc cgcctgcagg gtcgcgagcg cgctgggctc
     5401 ggcaaagtcc gccgaccagg agtcgttgcc gtcgatggcc aactgcacgt ggccgccgct
     5461 ggcggtgacc gtcagcttcg cgttcgcgtt gctgtacgcg tacgactgcc cggcgccgat
     5521 gtagtcgccc gcatcgctct gcagcgtgac gaaggacgcg gcgccgttgg cgccgatggc
     5581 ggtcgacgcc gtcgcggtgg cggcgttgtt ggccgcgacc ggatcgccgc tgggcgtcgc
     5641 gtacatggtg ttgctgaccc tgccaagcgc gttggcggcg atcgtcgaag gcacggtgaa
     5701 caccagggtg ccgccggcgg gcagggtggg caccgtcatg gcgccgccca gggtcgccgg
     5761 gcacgtggcg ccgccgctgg ccacgcaggt gacctggccg agcgtctggc tggcgtcgac
     5821 gttgtcctgc agcgtgaggt tgctcgacgc atcggggccg gcgttgctca ccgtcatcgt
     5881 gtaggtggcc acgccgccgc tggtcagcgt cgtggtggga ccctgcgcgg tcacctgcac
     5941 gtcggcgctg aacgcctgca gcgtcatcgc gacctgttcg gccgacgtcg gcgg
//
LOCUS       DATCRO010000369         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_228746, whole genome shotgun sequence.
ACCESSION   DATCRO010000369 DATCRO010000000
VERSION     DATCRO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228746"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1826)
                     /locus_tag="VIN75_10070"
     CDS             complement(315..1826)
                     /locus_tag="VIN75_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8974553.1"
                     /translation="MHDSLMQLMYGFGIALQPANLMWCVFGVLVGNLIGVLPGMGPLS
                     TISMLLPIVAVIAPVPGILMLAGIFYGSQYGGAIGAILLNLPSHPPHAVTCLDGYPLT
                     LQGRGGTALGITMMASFFAASVGIVVMIFLSPLLVKAADSFGPADICAIMLLGLLAGA
                     TMARGSPLKGVAMTVLGLLVGVAGTDEISGTARYTLGLSELRDGVELGGLTMGLFGIA
                     DFLVSVNRTTVNPANARVSMRDMRPSRAEIRQSFWPMLRGTGVGTAFGALPGTGPTIT
                     TFLAYALERRWSRTPERFGKGALEGVAAPEAASHSKTQVDFIPTMSLGIPGDAVMALI
                     LGALIMKGLQPGPQMVTDHPDIFWGLIASFWIGNVLLVILNVPLIGVWVSMLRVPYRF
                     LYPAALFFIGVGVYSTRNNLFDVAITAAFGVIGAVLLALEFPLSPIVLGFVLGPQLEP
                     NFRRAMLLSRGDLSVFLTRPVASGFVAASALLVLGQLLAWGRRRRRPAPTLAR"
     gene            complement(1831..2307)
                     /locus_tag="VIN75_10075"
     CDS             complement(1831..2307)
                     /locus_tag="VIN75_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8974554.1"
                     /translation="MNKMTKDHVGGALLLAVGVAVVVAGVGYGLGTLRQMGSGFFPVV
                     LGVLLALVGVALLLSAGRATGSVADEPPAEMAHLAGPVVQWRGWLCIVGAALAFVVLG
                     EHGGLVPASFASVFIAAMGDRGNSVRSAAALAAGLTLAGVVVFHYGLHLLLPLFAW"
     gene            2371..2850
                     /locus_tag="VIN75_10080"
     CDS             2371..2850
                     /locus_tag="VIN75_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY8974555.1"
                     /translation="MGIEIERKFLVVGDAWRAAPALAYAQGYLNRDKQRTVRVRIVQQ
                     QAWLTIKGASAGAVRAEFEYPIPVSDAQQLLALCDGPLVRKLRRRIEHAGHAWEIDEF
                     QGDNAGLVVAEIELPAEDAAFERPAWLGDEVTHDPRYFNSNLAAAPYSTWPDARPRA"
BASE COUNT          508 a         1078 c         1021 g          336 t
ORIGIN      
        1 cagcagcacg agggacgaga cgaccagcaa cgggaagcgc gacggggtca tggcggcctg
       61 ggtccggtgg gaacgtttcc gatcctacgc accacgggcc gcgggccgaa ccccgcaaag
      121 accctgagtc cgcgtgccga acgcggcctc cttgggcggc ttcacggtgg ccgcgcccgc
      181 cgtggcggcg agcagcaggg acgagacgac cagcaacggg atgcgcgacg ggatcatggc
      241 ggcctgcgtg cggtgggaac gctttcgatt gtgcgcaccg gccgtcgcgc cgccgacccc
      301 gcaaagaccc tgagtcagcg tgccaaagtt ggcgcggggc gacgccggcg gcgtccccac
      361 gcgagcagct gccccagcac cagcagcgcg ctggccgcga cgaagccgct ggccaccggt
      421 cgcgtcagga acaccgacag gtcgccgcgc gacagcagca tcgcgcgccg gaagttcggc
      481 tccagctgcg gccccagcac gaagcccagc acgatgggcg acagcgggaa ctcgagcgcc
      541 agcagcaccg ccccgatcac gccgaaggcc gcggtgatgg ccacgtcgaa caggttgttg
      601 cgggtgctgt agacgcccac gccgatgaag aacagggccg ccggatacag gaaccggtac
      661 ggcacgcgca gcatgctcac ccacacgccg atcagcggca cgttcaggat caccagcagc
      721 acgttgccga tccagaagct ggcgatcagg ccccagaaga tgtcggggtg gtcggtgacc
      781 atctgcggcc cgggctgcag gcccttcatg atcagcgcgc ccaggatcag cgccatcacg
      841 gcgtcgcccg gaatgcccag cgacatcgtc gggatgaagt cgacctgcgt cttggagtgc
      901 gacgcggcct cgggggccgc cacgccctcc agcgcgccct tgccgaagcg ctcgggcgtc
      961 ctggaccacc ggcgctccag cgcgtaggcc aggaaggtgg tgatggtcgg gccggtgccc
     1021 ggcagcgcgc cgaaggccgt gcccacgccg gtgccgcgca gcatcggcca gaacgactgg
     1081 cggatctcgg cccgggacgg gcgcatgtcg cgcatcgaga cgcgcgcgtt ggcggggttg
     1141 accgtggtgc ggttgacgct gaccaggaag tcggcgatgc cgaacaggcc catcgtcagg
     1201 ccacccagct cgacgccgtc gcgcagctcg gacagcccca gcgtgtagcg cgcggtgccg
     1261 ctgatctcgt cggtgccggc caccccgacc agcaggccga gcaccgtcat cgccacgccc
     1321 ttgagcggcg agccgcgcgc catcgtcgcg cccgccagca ggcccagcag catgatggcg
     1381 cagatgtcgg ccgggccgaa gctgtcggcc gccttcacca gcagcggcga caggaagatc
     1441 atcacgacga tgcccaccga ggccgcgaag aacgacgcca tcatcgtgat gcccagcgcc
     1501 gttccgcccc ggccctgcag cgtcagcggg tagccgtcga ggcaggtcac cgcgtgcggc
     1561 gggtggctgg gcaggttcag caggatcgcg ccgatcgccc cgccgtactg cgagccgtag
     1621 aagatgccgg ccagcatcag gatgcccggc accggcgcga tgacggccac gatgggcagc
     1681 agcatcgaga tcgtcgacag cggccccatc ccgggcagca cgccgatcag gttgcccacc
     1741 agcacgccga acacgcacca catcaggttc gcgggctgca gcgcgatgcc gaagccgtac
     1801 atcagttgca tcaggctgtc gtgcatcgcg ctaccaggcg aacagcggca gcagcaggtg
     1861 caggccgtag tggaagacga cgacgccggc gagcgtcagc ccggcggcca gcgcggcggc
     1921 cgagcggacg ctgttgccgc ggtcgcccat cgccgcgatg aacaccgacg cgaacgacgc
     1981 cggcaccagc cccccgtgct cgcccagcac gacgaaggcg agcgcggcgc cgacgatgca
     2041 cagccagccg cgccactgca ccaccgggcc ggccaggtgc gccatctcgg cgggcggctc
     2101 gtcggcgacg ctgcccgtcg cgcgcccggc gctcagcagc agcgcgacgc cgaccagcgc
     2161 gagcagcacg cccagcacga cgggaaagaa gcccgagccc atctggcgca gcgtgcccag
     2221 gccgtagccg acgcccgcga ctaccacggc caccccgacc gcgagcagca gcgcaccgcc
     2281 gacgtggtcc ttcgtcatct tgttcatggt ccccactcta tcgccgcggc acgacgacaa
     2341 tgcggcctgg gcaatggcgg gagcaccgtg atgggcatcg agatcgagcg caagttcctg
     2401 gtcgtgggcg acgcctggcg cgccgcgccg gcgctcgcct acgcccaggg ctacctcaac
     2461 cgcgacaagc agcgcacggt gcgcgtgcgc atcgtgcagc agcaggcctg gctgacgatc
     2521 aagggcgcga gcgccggcgc cgtgcgggcc gagttcgaat acccgatccc ggtgtccgac
     2581 gcgcagcagc tgctcgcgct gtgcgacggc ccgctggtgc gcaagctgcg ccgccggatc
     2641 gagcatgcgg gccacgcctg ggagatcgac gagttccagg gcgacaatgc cggcctggtg
     2701 gtcgccgaga tcgaactccc cgccgaggac gcggccttcg agcggcccgc ctggctgggc
     2761 gacgaggtga cgcatgatcc gcgctacttc aactccaacc tggccgcggc gccctattcg
     2821 acctggcccg acgcgcgtcc ccgcgcgtga cgcccgcgcg cgcgacctcg agaacaactt
     2881 tccaacggac aacaagacat ggccaactcc cccgacgtca ccagcatggc gctgttctgc
     2941 gac
//
LOCUS       DATCRO010000370         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1050096, whole genome shotgun sequence.
ACCESSION   DATCRO010000370 DATCRO010000000
VERSION     DATCRO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050096"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..126)
                     /locus_tag="VIN75_10085"
     tRNA            complement(51..126)
                     /locus_tag="VIN75_10085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:Pro,seq:tgg)
     gene            complement(132..210)
                     /locus_tag="VIN75_10090"
     tRNA            complement(132..210)
                     /locus_tag="VIN75_10090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Arg,seq:acg)
     gene            complement(588..1184)
                     /locus_tag="VIN75_10095"
     CDS             complement(588..1184)
                     /locus_tag="VIN75_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974556.1"
                     /translation="MNATPAALACAALCAATVLVPAPMRAQAAPVRDGLHDFDFLMGH
                     WHAHLRRRVDPLTGSAQWIEYDGTSVISKIWDGRANTEEFKVAVVARDAQTEAAAKRP
                     IIDAQTLRLYDPATGLWSLWLADAARGKLTGPPTIGAFRDGRGEFLDAEDFKGRPIIV
                     RYVWTRPTPTSAHFEQSFSPDWGRTWEVNWICDLTPEA"
     gene            complement(1270..2217)
                     /locus_tag="VIN75_10100"
     CDS             complement(1270..2217)
                     /locus_tag="VIN75_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEY8974557.1"
                     /translation="MRPRLRMTWVAGVAAAAALAAAGWSVLPAAAPRADAPASAGRDG
                     APAVPASLAGTTPGGAAHATADDALVLDPSLIRLFDYWLTTVGEQPIASIRSQVEHDL
                     DGRLGPRAARQAKDLFARYVQFKEALKTQRPAKPVGRSVDILREGLRRMQALRASYFS
                     QAETLALFGPQDAEASAALARMAVEQDPALDAATRQQRLAELDARLPASVRDARAASM
                     AVVHLEEAAQKIRAAGGSEDDVYRMRAAATSPEAANRLAEVDREEAAWKARIAAYQAQ
                     RGAVLAAPGDDAAHASAMAELRTRLFTPDEQRRLAAYED"
     gene            complement(2219..3145)
                     /locus_tag="VIN75_10105"
     CDS             complement(2219..3145)
                     /locus_tag="VIN75_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEY8974558.1"
                     /translation="MNKTILKWLAVAILALSALPSWASGYAQTRYPIILVHGLFGFDN
                     IGPLEYFYGIPEDLRANGAQVYVAQVSAANSTEVRGEQLLTYVKQVLAVTGASKVNLI
                     GHSHGGPTVRYVASVAPNLVASVTSVAGPNQGAALADALNGTLPAGSLSRTVVATVVN
                     GFASLIGLISGNNASQNSSAALNSLSTAGMAKFNAAHPEGLPTTACGQGPAVASNGVR
                     YYSWSGAQPFTNALDPLDAGLTVVSVVYGGARNDGLVGSCSSHLGTVLRDDYGMNHLD
                     EVNQTAGIVNWFEVDPKSVYRNQANRLKNLGL"
     gene            3478..4218
                     /locus_tag="VIN75_10110"
     CDS             3478..4218
                     /locus_tag="VIN75_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8974559.1"
                     /translation="MARLTAAQRQTMLDGPWFGTLPPGPRDEVLDVATLRHLGEGEVV
                     YAKDRPGDAWYGILGGAIRLGASGPDGRQGLLTFLQPGTWFGDTSLFDRLPRPHDAVA
                     HCPSTLLAVSAAQFEGLLDRYPVLYRHFVDLYCQRSRLMFLALESWTAFSLDERLAMH
                     LVHLANGHGLRDGPDVAINLHLPQEQLAQLLGVTRQRISQILHEWERLGRVHVRYGRV
                     VLDGQWFASDPTAPHLHAVPMASLPLAH"
     gene            4255..4659
                     /locus_tag="VIN75_10115"
     CDS             4255..4659
                     /locus_tag="VIN75_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974560.1"
                     /translation="MRFEVENKPPIEGPSARRVRAGILSLRNRGRSSFATLTDERGNY
                     LLAAGGALTCMLERRDVDAGRMLRAFVDKPGVTVPDGTILAFGAGQIRLMADEWLTAP
                     QVAEAFDAFLLGKPWPAVLRWRDQGDVLPRTA"
     gene            complement(4667..>4946)
                     /locus_tag="VIN75_10120"
     CDS             complement(4667..>4946)
                     /locus_tag="VIN75_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8974561.1"
                     /translation="LSGDRAAMQVQLSHVLDGYRAFMDFNPAELALVEPLRALRMIHH
                     SAWLADRWDDPAFPAAFPWFGTPAYWDQQTTQLREQLEHMTLPPLAAF"
BASE COUNT          698 a         1690 c         1818 g          740 t
ORIGIN      
        1 tctgagaccg gtgcgtctgc caattccgcc acgaggggac ggatgaaaag tggtctcggc
       61 ggcagggttc gaacctgcga ccctctgctc ccaaagcaga cgcgctgcca gctgcgctac
      121 accgaggggg ttggcgcctc gtgcacgatt cgaacgtgcg acctccggct tcgtaggccg
      181 gcgctctgga tccaactgag ctaacgaggc gaagaaaaga gaagggcccg gactctcgcg
      241 aatccgggcc cctcgaaaag ggttggcgat ggcgcgcgat caggcgcggg cttgtcccgg
      301 aaggctgggt cgttgcggtc gctgcagcgc cggctgtcgc gccaagcgcg cgagcggtcg
      361 ccgggcggcg agggctttgc gatgcgatag gcggttgttc acgacgtgtc cttgaaagat
      421 gcgttgctgc ggatcagcga atgatgaagc tgtaaagact ctttacttcg ttggcaagcc
      481 aagggtggca acgtgttgcg tgcgacgcac gcaagccagg ccgtcatcct gcgcgccgga
      541 tgacagggtt ggcgttccac ggatgcacac ccgtgaggac gacaggctca cgcctccggt
      601 gtcaggtcgc agatccagtt cacttcccag gtgcggcccc aatccggcga gaacgactgc
      661 tcgaagtgcg cggaggtcgg cgtcgggcgc gtccagacgt agcgcacgat gatggggcgg
      721 cccttgaagt cctcggcatc gaggaactcg ccgcggccat cgcggaaggc gccgatggtg
      781 ggcggcccgg tcagcttgcc gcgcgcggcg tcggccagcc agaggctcca caggccggtg
      841 gcggggtcgt acaggcgcag cgtctgcgcg tcgatgatcg gccgcttggc ggctgcctcg
      901 gtctgcgcgt cgcgcgcgac gacggcgacc ttgaattcct cggtgttggc gcggccgtcc
      961 cagatcttgg agatcaccga cgtgccgtcg tactcgatcc actgcgccga gccggtcaac
     1021 ggatccaccc gccggcgcag gtgcgcatgc cagtggccca tgaggaagtc gaagtcgtgc
     1081 aggccgtcgc gcacgggcgc ggcctgcgcg cgcatggggg cgggcacgag gacggtcgcg
     1141 gcgcacaaag cggcgcaggc gagggcggcg ggagtggcgt tcatgtcggg ctccgatcgt
     1201 tggggaggcc cgatgctgcg cggccaagtc ggccaacgcg tgtcatgttt ttccgcgacg
     1261 cccgccgttt caatcttcgt aggcggccag ccgccgctgc tcgtccggcg tgaacagccg
     1321 agtgcgcagc tccgccatcg cggacgcgtg cgccgcgtca tcgcccggcg ccgccagcac
     1381 cgcgccgcgc tgcgcctggt aggcggcgat gcgcgccttc cacgcggcct cctcgcgatc
     1441 gacctcggcc agccggttgg cggcctcggg cgaggtcgcg gcagcgcgca tgcggtacac
     1501 gtcgtcctcg ctgccgccgg cggcgcggat cttctgtgcg gcttcctcga ggtggacgac
     1561 ggccatcgac gcggcgcgcg cgtcacgcac gctggcgggc aggcgcgcgt cgagctcggc
     1621 caggcgctgc tggcgcgtgg ccgcgtcgag cgcgggatcc tgttcgacgg ccatgcgcgc
     1681 cagtgcggcg gacgcctcgg cgtcctgcgg gccgaacagc gcgagcgtct cggcctggct
     1741 gaagtacgac gcgcgcagcg cctgcatccg gcgcaggccc tcgcgcagga tgtcgacgct
     1801 gcggcccacg ggcttggccg gacgctgtgt cttgagcgcc tccttgaact ggacgtagcg
     1861 cgcgaacagg tccttggcct ggcgcgccgc gcgcggcccg agccggccgt cgaggtcgtg
     1921 ctcgacctgc gaccggatcg acgcgatcgg ctgctcgccg accgtggtga gccagtagtc
     1981 gaacaggcgg atcagcgacg ggtcgagcac gagcgcgtcg tcggccgtcg cgtgcgccgc
     2041 cccgcccggc gtggtgcccg cgagcgacgc cgggacggcc ggcgccccgt cgcgcccggc
     2101 ggacgcgggc gcgtccgcgc gcggcgccgc ggcgggcagg acggaccacc cggccgcggc
     2161 cagcgccgcc gccgcggcca cgcccgcgac ccacgtcatg cgcaggcgcg gacgcatgtc
     2221 acagccccag gttcttcagg cgattggcct ggttgcggta cacgctcttg gggtcaacct
     2281 cgaaccagtt gacgatgccc gcggtctggt tgacctcgtc gaggtggttc atgccgtagt
     2341 cgtcgcgcag gacggtgccc aggtggctgc tgcacgatcc gacgaggccg tcgttcctgg
     2401 cgccgccgta gaccacggac acgaccgtca gccccgcgtc gagcgggtcg agcgcgttcg
     2461 tgaagggctg cgcgccgctc cacgagtagt agcgcacgcc gttggaggcc acggccgggc
     2521 cctggccgca cgcggtcgtg ggcaggccct ccggatgcgc ggcgttgaac ttggccatgc
     2581 cggccgtcga cagcgagttc agcgcggccg acgagttctg cgacgcgttg ttgcccgaga
     2641 tcagcccgat cagcgacgcg aagccgttga cgacggtggc gacgacggtg cgcgacagcg
     2701 agcccgccgg cagcgtgccg ttgagcgcat ccgccagcgc ggcgccctgg ttcgggccgg
     2761 cgacgctggt gaccgaggcg accaggttgg gcgccaccga cgcgacgtag cgcaccgtgg
     2821 ggccgccgtg gctgtggccg atcaggttga ccttggacgc cccggtcacg gccagcacct
     2881 gcttgacgta ggtgagcagt tgctcgccgc gcacctcggt ggagttggcg gccgatacct
     2941 gggccacgta tacctgggcg ccgttggcgc gcaggtcctc ggggatgccg tagaagtact
     3001 ccagcgggcc gatgttgtcg aagccgaaca ggccgtgcac caggatgatc gggtacctgg
     3061 tctgcgcgta gcccgaggcc cacgagggca gggcggacag ggcgaggatg gccacggcca
     3121 gccatttcag gatcgtcttg ttcattgcag tcgtctccga gattcgttga gtggtgggcg
     3181 cccgtgcaag gcgcccgtgc aggtggcgtg gacgtgtgcg ggcgcacgca tccacgccga
     3241 acccctccag gttcgatctg tgtcgaagcg gtctcaactg aggtcgagcg agatgccggg
     3301 cctgcgcggc ggtggcgcgg gcgaagggcg tcgtggcatg ggtagcactg cggataggca
     3361 gcgggcgcag acgagtcgaa catcgcgttc gaaccccgca acctcagtac gaacgatcgt
     3421 acttttgctg cggcgcgtcg tctgtctctg atttgcaatt caaaaaggga agaccctatg
     3481 gcccgactga cagccgcgca gcggcagacg atgctcgacg gcccctggtt cggcacgctg
     3541 ccgccgggcc cgcgcgacga ggtgctggac gtcgcgacgt tgcgccacct gggcgagggc
     3601 gaggtcgtct atgccaagga ccggcccggc gatgcctggt acggcatcct gggcggcgcg
     3661 atccggctcg gcgccagcgg ccccgatggc cgccagggcc tgctcacctt cctgcagccg
     3721 ggcacgtggt tcggcgacac ctcgctgttc gatcgcctgc cgcggccgca cgacgccgtc
     3781 gcgcactgcc cgtcgacgct gctggcggtg tcggccgcgc agttcgaggg cctgctcgat
     3841 cgataccccg tgctgtatcg gcacttcgtc gacctgtact gccagcgctc gcggctgatg
     3901 ttcctggcgc tcgaatcgtg gacggcgttc tcgctcgacg agcgcctcgc gatgcacctg
     3961 gtgcacctgg ccaacggcca tggcctgcgc gacggccccg acgtcgcgat caacctgcac
     4021 ctgccgcagg aacagctcgc gcagctgctg ggcgtcacgc gccagcgcat cagccagatc
     4081 ctgcacgagt gggagcgcct gggccgcgtg cacgtgcgct acgggcgcgt cgtcctcgac
     4141 ggccagtggt tcgcgagcga tccgaccgcg ccgcacctgc acgcggtgcc catggcgagc
     4201 ctgccgctcg cgcactgaac ggtcgcacgg gacgggcgcg cgacaatcgc cggaatgcga
     4261 ttcgaagtcg agaacaagcc cccgatcgaa gggccctccg ccaggcgcgt gcgcgccggc
     4321 atcctgtcat tgcgcaaccg cggccgctcg tcgttcgcca cgttgaccga cgagcgcggc
     4381 aactacctgc tcgcggccgg cggcgccctc acgtgcatgc tggagcggcg cgacgtcgac
     4441 gccggccgca tgctgcgcgc gttcgtcgac aagcccggcg tgaccgtccc cgacggcacc
     4501 atcctcgcct tcggcgccgg ccagatccgg ctgatggccg acgaatggct caccgcaccc
     4561 caggtcgccg aggccttcga cgcgttcctg ctcggcaagc cgtggcctgc ggtgctgcgc
     4621 tggcgcgacc agggggacgt gctgccccgg acggcatagg gcctgctcag aacgccgcga
     4681 gcggcggcaa cgtcatgtgc tccagctgct cgcgcagctg cgtggtctgc tggtcccagt
     4741 aggccggcgt gccgaaccac gggaacgcgg cgggaaaggc cgggtcgtcc cagcggtcgg
     4801 ccagccaggc gctgtggtgg atcatgcgca gcgcgcgcag cggctcgacg agggcgagct
     4861 cggcggggtt gaagtccatg aacgcgcggt agccgtcgag cacgtgggag agctggacct
     4921 gcatcgccgc gcgatcgccg gacagc
//
LOCUS       DATCRO010000371         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_352532, whole genome shotgun sequence.
ACCESSION   DATCRO010000371 DATCRO010000000
VERSION     DATCRO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352532"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="VIN75_10125"
     CDS             <1..2297
                     /locus_tag="VIN75_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HEY8974562.1"
                     /translation="MRLIRILACAALAVLAQVPGHAAINASGLGAAYDAGQTSINFKV
                     YSSRATRIELWLYAAGTGQAEKLKVVMTKGSGNLWSANVTTSALTAAGISGTVYYGYR
                     AWGPNWTYNASWTKGSGLGFVADVDASGNRFNPNKLLMDPYAKELSQDPTNPSNQDGT
                     VYASGANYRLMDSGTVAPKGIVFLPDTQSIGTKPTRAFKDDVVYEVHVRGLTMNDTSI
                     AAAYRGTYKGAGLKAAWLASLGVTAVEFLPVQETQNDTNDVVPNTDAGDNYWGYSTLN
                     WFAPDRRYSSDKTAGGPTKEFKAMVKAFHDAGIKVMLDVVYNHSGEGGAWSSTDTTTY
                     NVQSYRGLDNPTYYELTSDNQSSYDNTGVGGNYNTYNTIAQNLIVDSLAYWRDTMGVD
                     GFRFDLAAVLGNTCTNGCFNYNKTDAGTALNRIVAEMTPRPAGGGTGTDFVAEPWAIG
                     TGTYQLGNFPSGWSEWNGSYRDLVRQAQNELGSVTVTTGQLATRFAGSSDLFGDDGRK
                     PWNSVNFLVVHDGFTLGDLYRCNGKNNSQAWPYGPSDGGEDNNNSWDQGSVATAQRQA
                     ARTGFALMMTSAGVPLMTGGDEYLRGLNCNNNPYNLDSSANWLNWSRTTDQTNFQNFA
                     QAMIAFRKAHASLRPAGFYSGSDNNANGLQQLAWFAPSGAIADSTYFNDANNHAIAWR
                     IDGSEFGDTAAALYVGYNGWSGAVNFTLPAPPSGKSWYRVTDTCTWAEGPGQVRAPGS
                     EDAIGGTGTVYSVCGRGALVLIAK"
     gene            2386..2967
                     /locus_tag="VIN75_10130"
     CDS             2386..2967
                     /locus_tag="VIN75_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974563.1"
                     /translation="MPSYADVTLQTARLHLRMPTLADTDAIFAMRSDPVVQRYGSHPP
                     WTDRQAAIDWIERNGRGMAAGEHAQFVIERRDDGVVIGSCILYGLDAQCRRAEVGYAL
                     ARAEWGKGYANEAVSTMLDWGFAHLDLNRIEADIDPRNEPSARALERLGFTREGHLRE
                     RWIVDGEVSDSWIYGLLAREWRAARAPLAGATA"
     gene            complement(3166..>3352)
                     /locus_tag="VIN75_10135"
     CDS             complement(3166..>3352)
                     /locus_tag="VIN75_10135"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEY8974564.1"
                     /translation="DTRELHADAVDVTLGGVGSVKVWAGQSLDASVGGVGSLTYYGEP
                     KQVNTRGGGLGSISKGK"
BASE COUNT          579 a         1208 c         1105 g          460 t
ORIGIN      
        1 cgatgagact gatccgcatc ctggcctgcg ccgcgctggc cgtcctcgcc caggttcccg
       61 gccacgccgc catcaacgcc tcggggctgg gcgccgccta cgacgccggg cagacgtcga
      121 tcaacttcaa ggtgtactcg tcgcgcgcca cgcgcatcga actgtggctg tacgcggccg
      181 gcacgggcca ggcggagaag ctcaaggtcg tgatgaccaa gggctcgggc aacctctggt
      241 cggccaacgt caccacctcc gcgctgaccg cggcgggcat cagcggcacc gtctactacg
      301 gctatcgcgc ctggggtccg aactggacgt acaacgcgtc gtggaccaag ggttcgggcc
      361 tgggcttcgt cgccgacgtc gatgcgtcgg gcaaccgctt caacccgaac aagctcctga
      421 tggacccgta cgccaaggag ctgagccagg atccgaccaa cccgtccaac caggacggca
      481 ccgtctacgc gtccggcgcc aactaccggc tgatggacag cggcaccgtc gcgcccaagg
      541 gcatcgtgtt cctgccggac acgcagtcca tcggcaccaa gcccacgcgc gcattcaagg
      601 acgacgtggt ctacgaggtg cacgtgcgcg gcctgacgat gaacgacacc agcatcgcgg
      661 ccgcctatcg cggcacgtac aagggcgcgg gcctgaaggc cgcgtggctg gccagcctgg
      721 gcgtgacggc ggtggaattc ctgcccgtcc aggagacgca gaacgacacc aacgacgtcg
      781 tgcccaacac cgacgccggc gacaactact ggggctactc cacgctcaac tggttcgcgc
      841 cggaccgccg ctattcgtcc gacaagacgg cgggcggccc gaccaaggaa ttcaaggcga
      901 tggtcaaggc gttccacgac gccgggatca aggtgatgct ggatgtcgtc tacaaccact
      961 ccggcgaagg cggcgcgtgg tcgtccaccg acaccaccac ctacaacgtg cagagctacc
     1021 gcggcctgga caacccgacc tactacgagc tcaccagcga caaccagtcc agctacgaca
     1081 acaccggcgt cggcggcaac tacaacacct acaacacgat cgcgcagaac ctgatcgtcg
     1141 actcgcttgc gtactggcgc gacacgatgg gcgtggacgg cttccggttc gacctggccg
     1201 cagttctggg caacacctgc accaacggct gcttcaacta caacaagacc gacgccggca
     1261 ccgcgctgaa ccgcatcgtc gccgagatga cgccgcgccc cgcgggcggc ggcaccggca
     1321 ccgacttcgt cgccgagcca tgggcgatcg gcaccggcac ctaccagctg ggcaacttcc
     1381 ccagcgggtg gtcggagtgg aacggcagct accgcgacct ggtgcgccag gcgcagaacg
     1441 agctgggctc ggtgacggtc acgaccggcc agctggccac gcgcttcgcg ggctcgtccg
     1501 acctgttcgg cgacgacggc cgcaagccat ggaactcggt caacttcctg gtcgtgcacg
     1561 acggcttcac gctcggcgac ctgtaccgct gcaacggcaa gaacaactcg caggcctggc
     1621 cgtacggtcc atcggacggc ggcgaggaca acaacaacag ctgggaccag ggcagcgtcg
     1681 ccaccgcgca gcgccaggcc gcgcgcaccg gcttcgcgct gatgatgacc tcggccgggg
     1741 ttccgctgat gaccggcggc gacgagtacc tgcgcgggct gaactgcaac aacaacccgt
     1801 acaacctcga ctccagcgcg aactggctga actggtcgcg caccaccgac cagaccaact
     1861 tccagaactt cgcgcaggcg atgatcgcgt tccgcaaggc gcacgcgtcg ctgcggccgg
     1921 cgggcttcta ttcgggcagc gacaacaacg ccaacggcct gcagcagctc gcctggttcg
     1981 cccccagcgg cgccatcgcc gacagcacct acttcaacga cgccaacaac cacgcgatcg
     2041 cctggcgcat cgacggcagc gagttcggcg acaccgccgc ggcgctgtac gtcggctaca
     2101 acggctggag cggcgccgtg aacttcacgc tgcccgcgcc gccgagcggc aagagctggt
     2161 atcgcgtgac cgacacctgc acctgggccg aaggtcccgg ccaggtgcgc gcgcccggct
     2221 cagaggacgc gatcggcggc accggcaccg tctacagcgt gtgcgggcgc ggcgcgctgg
     2281 tgctgatcgc caagtagcgg gccggcgaat tggtccggcc gcccgttgcc ctgcatctgg
     2341 cgcgccggac tgaagtcgcc gcggcgcgca tgtgagaatc ggccgatgcc cagctacgcc
     2401 gatgtcacgc tgcagaccgc gcgcctccac ttgcggatgc cgacgctcgc cgacacggac
     2461 gcgatcttcg cgatgcgttc cgaccccgtc gtgcagcgct acggcagtca tccgccctgg
     2521 acggaccggc aggcggccat cgactggatc gagcgcaacg ggcgcggcat ggccgcgggc
     2581 gagcacgcgc agttcgtgat cgagcggcgc gacgacggcg tcgtcatcgg cagctgcatc
     2641 ctgtacggcc tcgatgcgca atgccggcgc gccgaggtcg gctacgccct ggcccgcgcc
     2701 gaatggggca agggctacgc gaacgaggcg gtgagcacga tgctcgactg gggcttcgcc
     2761 cacctcgacc tgaaccgcat cgaggccgac atcgatccgc gcaacgaacc ctcggcgcgc
     2821 gcgctcgagc gcctgggctt cacgcgcgag gggcacctgc gcgagcgctg gatcgtcgac
     2881 ggcgaggtca gcgattcgtg gatctacggc ctgctcgcgc gcgagtggcg cgcggcgcgc
     2941 gcgccgctcg cgggcgcgac ggcctagcct tcggcctctg gctcggcgtc gatgcgcgcc
     3001 gggatcgtcc cgagcgccag ttcggcgctg ttgaacgcgc cttcgatgaa gccctggtag
     3061 tggcgggcgt gtccatgtac agcaggatca tcccgtggcc gtcgcggtcg gcgagttgac
     3121 accgcaagac ctggcaccgg ctcggcaccg gccgtcggcc gggcgtcact tgcccttgga
     3181 gatgctcccc agtccaccgc cgcgcgtgtt gacctgcttg ggttctccgt agtacgtcag
     3241 cgagccgacg ccgcccacgc tggcgtccag cgactggccg gcccagactt tcacggagcc
     3301 gacgccgccg agggtgacgt cgaccgcgtc ggcatgcagc tcgcgcgtgt cg
//
LOCUS       DATCRO010000372         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_48990, whole genome shotgun sequence.
ACCESSION   DATCRO010000372 DATCRO010000000
VERSION     DATCRO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48990"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VIN75_10140"
     CDS             complement(<1..1338)
                     /locus_tag="VIN75_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY8974565.1"
                     /translation="MSPDRIRLLLIEDSPADARLIRLMLEQSWAVHIHCDWVDNRTEG
                     IERLRAGGYDVVLLDLGLPESTGLETLRVVRRESDVPTLVVMSGSSDEEVALQALQAG
                     AQDYLIKGHVDGAALARSIRYAIGRTQAERALRARTDEQRQQAKYLRTLIDLLPMFAW
                     LKDTRSTYLTVNQATAAARGTTVERMEGRSDIDFWPREQAEAVRADDVQVMTSLQRKT
                     VEETLGTVDGDVWVETYKVPVLDEDGTVLGTVGVARDIRERKAAEAAHKVALAEAERL
                     ARQRSAFLAQMSHELRTPLNGILGFAQLLQQDKGLDERQVRGLRIIRESGQHLLTLIN
                     DILDLARIDAAKVDLNPTDIALPGFLAVVTDIVRVKAEEKSLLFVEHLAPDLPASVRA
                     DEKRLRQVLLNLLSNAVKFTTKGRVAVETRCAPDGIRIDVMDTGIGIRASDLEA"
     gene            complement(1335..1781)
                     /locus_tag="VIN75_10145"
     CDS             complement(1335..1781)
                     /locus_tag="VIN75_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974566.1"
                     /translation="MAGERSIRTIDILMVEDDPGDVRLTREALKGSKLLHSLNVVEDG
                     VAALDYLRRNAPYQDAVRPDLVLLDLNLPRKDGREVLAAMKQDDALRAIPVVILTTSQ
                     AEEDVLRAYNLNANCYVTKPVDFDQFMRIVRTIEEFWLNVVTLPPK"
     gene            complement(1825..>3608)
                     /locus_tag="VIN75_10150"
     CDS             complement(1825..>3608)
                     /locus_tag="VIN75_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974567.1"
                     /translation="RLADLATRQQAQLGSSLAHARASLLSVKVVTAIAGALAVIIAMA
                     MAYAFRRTVVRPLHRLTRVAERVTAGDLEARAQVESSDEVGVLARGINTMTARLSETI
                     RHLETIFAEAQDAKAAAEVANRAKSRFLASMSHELRTPLNAVLGYAQILQSEPGLSEF
                     QANRLDKIRTGGEHLLAMINDVLDLSRIEAGKVELQPQPMSLASLLGAICSVIRAAAE
                     RKGLSFVSDVDPDLPVAVDVDGKRLEQVLLNLLDNAVKFTERGQVCLRLRRLESTDPA
                     RARIRFEVADSGIGIDPAHLAALFKPFEQGDDTKRQHGGTGLGLAISQRIVAMMGGNI
                     EVQSQPGAGSLFGFAIDVALADSDAATGAPRGGEDPAAVWRRIVVVEGAPARDADLIE
                     QLLACGFEVTRADGVASALEQARTQLPDLVLVDIGTPQAQGIAAVRRLREDAMLRGIA
                     LIALAPAGAAHELADSGADALLQAPLELAAFAAAIDELRQRRSRGGASTAAAREAPPR
                     WVLPGAEELERLHELARIGNMRSIGERADHLAAINPDYQPFAHRLRDLAQRFQSRAIL
                     EWITELRRADAAASGVTPPVRGPTVGL"
BASE COUNT          494 a         1293 c         1219 g          602 t
ORIGIN      
        1 cgcctccaga tcggacgcgc gaattccgat gcccgtgtcc atcacgtcga tccgaattcc
       61 gtccggcgcg cagcgtgtct ccacggccac gcgccctttc gtcgtgaact tgaccgcgtt
      121 cgacagcagg ttcagcagga cctggcgcag gcgcttctcg tcggcgcgca cgctggccgg
      181 caggtccggc gccaggtgtt cgacgaacag cagcgacttc tcctcggcct tgacgcgcac
      241 gatgtcggtc acgacggcca ggaagccggg cagcgcgatg tcggtcgggt tcaggtccac
      301 cttggcggca tcgatgcgcg ccaggtccag gatgtcgttg atcagcgtga gcaggtgctg
      361 cccgctctcg cggatgatcc tgagcccgcg gacctggcgc tcgtccagcc ccttgtcctg
      421 ctgcagcagc tgggcgaagc ccaggatgcc gttgagcggc gtgcgcagct cgtggctcat
      481 ctgcgccagg aacgcgctgc gctgccgggc caggcgttcg gcctcggcga gcgcgacctt
      541 gtgggccgcc tcggccgcct tgcgctcgcg gatgtcgcgc gccacgccga ccgtcccgag
      601 caccgtgccg tcctcgtcga gcacgggcac cttgtacgtc tcgacccaga catcgccgtc
      661 gaccgtgccc aatgtctcct cgacggtctt gcgctgcagc gacgtcatga cctggacgtc
      721 gtcggcacgg accgcctcgg cctgctcgcg cggccagaag tcgatgtcgc tgcggccctc
      781 catccgctcc acggtcgtgc cgcgcgccgc ggccgtcgcc tggttgacgg tcaggtaggt
      841 gctgcgcgtg tccttgagcc acgcgaacat cggcagcaga tcgatcagcg tgcgcaggta
      901 tttggcctgc tggcgttgtt cgtcggttcg cgcgcgcagc gcccgctccg cctgcgtgcg
      961 cccgatcgca tagcggatgg accgcgccag cgcggcgccg tcgacgtggc ccttgatgag
     1021 gtagtcctgg gcgccggcct gcagggcctg cagcgccacc tcctcgtcgc tcgatccgct
     1081 catcaccacc agcgtcggca cgtccgactc gcggcggacc accctcagcg tctcgaggcc
     1141 cgtactctcg ggcaggccca gatccagcag cacgacgtcg taaccgccgg cgcgcaggcg
     1201 ttcgatgcct tcggtccggt tgtcgaccca gtcgcagtgg atgtgcaccg cccaggactg
     1261 ctccagcatg agccggatca gccgggcgtc ggccgggctg tcctcgatca gcagcaggcg
     1321 gatgcgatcc gggctcattt cggtggcagc gtgacgacgt tgagccagaa ctcctcgatg
     1381 gtccgcacga tccgcatgaa ctggtcgaag tccaccggct tggtcacgta gcaattcgcg
     1441 ttgaggttgt acgcgcgcag cacgtcttcc tcggcctgcg acgtcgtcag gatcaccacc
     1501 gggatggccc gcagcgcgtc gtcctgcttc atggcggcca gcacctcgcg gccatccttc
     1561 ctgggcaggt tcaggtccag gagcacgagg tcggggcgga cggcatcctg gtagggcgcg
     1621 ttgcgtcgca ggtagtcgag cgccgcgacg ccgtcctcca ccacgttcag gctgtgcagc
     1681 agcttgctgc ccttgagcgc ttcgcgcgtc aggcgaacgt ctccggggtc gtcctcgacc
     1741 atcaggatgt cgatcgtcct gatcgaacgt tctcctgcca tgtccctgct ccttgcagcg
     1801 cccgtccacc catcgtcgaa ttcactatag accgacggtc gggccgcgta caggcggagt
     1861 tacaccgctt gccgcggcgt cggcgcgacg cagctcggtg atccactcga ggatggcgcg
     1921 cgactggaag cgctgcgcca ggtcacgcaa gcgatgggcg aacggctggt agtccgggtt
     1981 gattgcggcc aggtggtccg cccgctcgcc gatgctgcgc atgttgccga tccgcgccag
     2041 ttcgtggagc cgttcgagtt cctccgctcc gggcaacacc caccggggcg gtgcctcccg
     2101 cgcggcagcg gtggatgcgc cgccgcggga ccggcgctgc cgcagctcgt cgatggcggc
     2161 ggcgaacgcg gccagctcga gcggcgcctg cagcaaggcg tcggcgcccg agtccgcgag
     2221 ttcgtgcgcg gccccggccg gcgccagcgc gatcagcgcg atcccgcgca gcatggcatc
     2281 ctcgcgcaag cgtcgaacgg ctgcgatgcc ttgcgcctgc ggcgttccga tgtcgaccag
     2341 gaccagatcc ggcaactgcg tgcgcgcttg ctccagcgcc gacgccacgc cgtcggcgcg
     2401 cgtcacttcg aatccgcagg ccagcagctg ctcgatcagg tcggcatccc gggccggcgc
     2461 gccttcgacc acgacgatcc tgcgccagac ggccgcaggg tcctcgccgc cgcgaggcgc
     2521 acccgtcgcc gcatcggaat cggccagcgc cacgtcgatc gcgaacccga acaggctgcc
     2581 cgccccgggc tggctctgca cctcgatgtt gccgcccatc atggcgacga tgcgctggct
     2641 gatcgccagg cccagtccgg tgcctccgtg ctggcgcttc gtgtcgtcac cctgctcgaa
     2701 gggcttgaag agcgcggcca gatgtgccgg gtcgatgccg atcccggaat cggccacctc
     2761 gaaccggatg cgcgcacgcg ccgggtccgt cgattccagg cggcgcaggc gcaggcacac
     2821 ctggccgcgt tcggtgaact tcacggcgtt gtccagcagg ttcagcagca cctgctccag
     2881 gcgcttgccg tcgacgtcca ccgccaccgg caggtcggga tcgacgtcgc tgacgaagga
     2941 caggcccttt cgttcggcag ccgcgcggat cacgctgcag atcgcgccga gcaggctcgc
     3001 caggctcatc ggctgcggct ggagttccac cttgccggcc tcgatgcgcg acagatcgag
     3061 cacgtcgttg atcatcgcca ggaggtgttc cccgccggtg cggatcttgt ccagccggtt
     3121 ggcctggaac tcgctcaagc cgggctccga ctgcaggatc tgggcgtatc cgagcaccgc
     3181 attgagcggc gtgcgcagtt cgtggctcat gctcgcgagg aaccggctct tggcgcggtt
     3241 ggcgacctcg gccgccgcct tggcgtcctg cgcctcggcg aagatcgtct ccaggtgccg
     3301 gatggtctcg gacagccgcg cggtcatcgt gttgatgccg cgggcgagga cgccgacctc
     3361 gtcgctcgat tcgacctgcg ccctcgcctc gagatcaccg gcggtgacac gctcggccac
     3421 gcgggtcagg cggtgcaacg gacgcacgac ggtgcgcctg aacgcatagg ccatggccat
     3481 ggcgatgatc accgcgagcg cgccggcgat ggccgtcacc accttgaccg acaacaggct
     3541 cgcgcgggca tgcgcgagac tgcttccgag ttgcgcctgc tggcgggtcg ccaggtcggc
     3601 caggcgcc
//
LOCUS       DATCRO010000373         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_442610, whole genome shotgun sequence.
ACCESSION   DATCRO010000373 DATCRO010000000
VERSION     DATCRO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442610"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2054)
                     /locus_tag="VIN75_10155"
     CDS             complement(<1..>2054)
                     /locus_tag="VIN75_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY8974568.1"
                     /translation="VIVEEAPPRPASPPASGDQVLLLSARSEAALDVAAGQLAAHLES
                     HPALPLADVAHTLSVGRKAHAFRRAVVAGGTAEAIAALRDAASPRAASGRAASRAQQL
                     VLMFPGQGAQYAGMGRNLHANDPVFAAAFDDCMEAFAGATDFDLRERMFSDDPQALAP
                     TAATQPAIFTLEYALARRLLALGARPHALVGHSVGEFVAAVIAGVMRLEDAARLVARR
                     GALMQAQPAGAMLSVRMGAADLAGRLGSGLSLAADNGPTACVAAGPFEEIAALQAALE
                     ANGIACRALQTSHAFHSAMMEPAVAPFEALVAKVALRAPQIPIFSTLTGQPLEDADAT
                     SPAYWARHLRGTVRFSPALRSAMAQLQNPLFVEAGPRNTLATLARQHGAKADSVAALL
                     HLEPGADADEARTLRLALARLWARGAEVELSRLTARAGAQRVRLPTTPFERKRCWVDI
                     APAPVPAPAASPAASPVVSSIAAAPVAAVVVPPISLEPIVTAATPSPLPPPAASPIDA
                     RLRALFEDISGLDMAQAEGQAPFGELGLDSLTLTQAATQIKKHFKVGVSFRQLMENYR
                     SFDALSAFLRESLPPEAAPAPAAPMAPAASAPVETAVSVMQAAVQQPMPAPMPVYMPV
                     QGSAVMGDAHLPQLISQQMELMRQQLALLSGAAAAAPLVTLQAVAPVQAVSAPAAVAP
                     AA"
BASE COUNT          288 a          678 c          841 g          247 t
ORIGIN      
        1 cggctgccgg tgcaaccgcc gcgggggcgg aaacggcctg caccggcgcc acggcctgca
       61 gcgtcaccag cggcgctgcg gcagcggcgc ccgacagcag cgcgagctgc tggcgcatca
      121 attccatctg ctgcgaaatc agctggggca gatgggcgtc gcccatgacg gcgctgcctt
      181 gcacgggcat gtagaccggc atcggcgccg gcatcggctg ctgcacggcc gcctgcatga
      241 cggagaccgc ggtttcgact ggcgcgctcg ccgccggagc catcggggcg gcgggggccg
      301 gcgccgcctc gggcggcagg ctctcgcgca ggaaggccga gagcgcgtcg aagctgcggt
      361 agttctccat cagctggcgg aagctcacgc cgaccttgaa gtgcttcttg atctgcgtgg
      421 ccgcctgcgt gagcgtgagc gagtcgaggc ccagttcgcc gaagggcgcc tgtccctcgg
      481 cctgggccat gtcgaggccg gagatgtctt cgaacagggc gcgcaggcga gcgtcgatgg
      541 gcgatgcggc tggcggagga agaggcgagg gcgtagcggc ggtcacgatc ggctccaggg
      601 aaatgggtgg gacgacgacg gcggcgacgg gcgcagccgc aatggacgaa acgacgggag
      661 aagcagcggg actggcggcg ggcgcaggga caggggccgg ggcgatgtcg acccagcagc
      721 gcttgcgctc gaaaggcgtg gtcggcaggc gcacgcgctg cgcgccggca cgggcggtca
      781 ggcgcgagag ctcgacctcg gcaccgcggg cccacaggcg cgcgagtgcc agtcgaaggg
      841 tgcgtgcctc gtcggcatcg gcgccgggct ccaggtgcag cagcgcagcg acgctgtccg
      901 ccttggcacc gtgctggcgt gccagcgtgg cgagcgtgtt gcgcgggccg gcctcgacga
      961 agagcgggtt ctgcagctgc gccatcgcgc tgcgcagggc cggcgagaaa cgcaccgtgc
     1021 cgcgcaggtg ccgggcccag taggccgggc tggtggcgtc ggcgtcttcg agcggctggc
     1081 cggtgagcgt cgagaagatc gggatctgcg gcgcgcgcag cgccaccttg gccaccagcg
     1141 cctcgaaggg cgcgacggcc ggttccatca tggccgagtg gaaggcatgc gaggtctgca
     1201 gcgcgcggca ggcaatgccg ttggcctcca gcgcggcctg cagcgcggcg atttcctcga
     1261 aggggccggc ggccacgcag gccgtggggc cgttgtcggc ggcgagcgaa agaccggacc
     1321 cgagccggcc ggcgagatcg gccgcgccca tgcgcaccga cagcatcgcg cccgcgggct
     1381 gcgcctgcat gagcgcgccg cggcgggcga ccagccgggc ggcatcctcg agccgcatca
     1441 cgccggcgat gacggccgcg acgaactcgc ccacgctgtg gccgaccagc gcatgcggac
     1501 gagcgcccag tgccaggagc cggcgggcga gtgcgtattc gagcgtgaag atcgcgggct
     1561 gcgtggcggc cgtgggtgcc agcgcctgtg ggtcgtcgct gaacatgcgc tcgcgcaggt
     1621 cgaagtccgt cgcgcccgcg aaggcttcca tgcagtcgtc gaaggctgcg gcgaacaccg
     1681 ggtcgttcgc atggaggttg cggcccatgc cggcgtattg cgcgccctgg ccggggaaca
     1741 tcagcaccag ctgctgtgcg cgcgaggccg cgcggccgct ggcggcgcgg ggcgacgcgg
     1801 catcgcgcag cgcggcgata gcttcggcag tgccgccggc caccacggcg cggcggaagg
     1861 cgtgggcctt gcggccgacc gagagggtgt gggccacgtc ggccagggga agtgcgggat
     1921 ggctttccag gtgtgcggcg agctggccgg cggcgacgtc gagcgccgcc tcggagcggg
     1981 ccgagagcag cagcacctgg tcgcccgagg ccggcggcga ggcgggccgc ggcggcgctt
     2041 cctcgacgat gacg
//
LOCUS       DATCRO010000374         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_589290, whole genome shotgun sequence.
ACCESSION   DATCRO010000374 DATCRO010000000
VERSION     DATCRO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589290"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VIN75_10160"
     CDS             complement(<1..251)
                     /locus_tag="VIN75_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974569.1"
                     /translation="MSATTQPRVVMQAKSLVKRYGSVTALDGSDFELRSGEILAVIGD
                     NGAGKSSLIKALSGATVPDSGEIQLDGVVTKFRSPIEARR"
     gene            complement(248..1183)
                     /locus_tag="VIN75_10165"
     CDS             complement(248..1183)
                     /locus_tag="VIN75_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974570.1"
                     /translation="MSVAVLGPVIALVLACVFFSTQSDHFLSGANFSLILQQVAVVGV
                     IAIGQTLVILTGGIDLSCGMLMALGGIVMTRFAAVNGMAPPLAILCGVGVTTLFGLVN
                     GLLVTRIRLPSFIVTLGTMNIAFAATQLYSGAQTVTDLPPMMTTLGDTFGIGSTQFAW
                     GTVVMLALYAIAWFMLRETAAGRHVYAVGNNPEATRLVGIATQRVLLIVYVVAGALYG
                     VASLLSVARTGVGDPNAGQTDNLDAITAVVLGGTSLFGGRGVVIGSLVGALVVGVFRN
                     GLTLMGVSSIYQVLVTGVLVILAVTADQLSRRGAR"
     gene            complement(1257..2258)
                     /locus_tag="VIN75_10170"
     CDS             complement(1257..2258)
                     /locus_tag="VIN75_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY8974571.1"
                     /translation="MLKIASSLLATAVLATASPLALAADQPVIGLITKTDTNPFFVKM
                     KEGATQMAKERGARLLTAAGKQDGDNAAQVTAIENMMTAGAKTILITANDSKAIVPAI
                     QKARAKGVMVIALDSPTDPQDATDALFATDNYKAGILIGEYAKAALGGKPAKIVTLDL
                     FPGHPVGAQRHNGFMKGFGLAAPDAKVNELGKSAEIVCSADSYGDQAKGQTAMENCLQ
                     KNPDVNLVYTINEPAAAGAYTALKRAGKEKGVIIVSVDGGCAGIRNVKAGVIAATSQQ
                     YPLKMAALGVAAGVDYATKGTKVSGYTDTGVTLIADKAMPGVESKDSKVGESLCWGK"
     gene            2415..2804
                     /locus_tag="VIN75_10175"
     CDS             2415..2804
                     /locus_tag="VIN75_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8974572.1"
                     /translation="MLLRLHHAAIICSDYEASKRFYTQVLGLRVIAEHWRAARRSWKL
                     DLGLPDGSQVELFSFEGAPPRPSYPEARGLRHLAFAVADVAAVKAHLQAHGVAVEDIR
                     VDEYTGRRFTFFADPDELPLEVYEDPA"
     gene            2854..3210
                     /locus_tag="VIN75_10180"
     CDS             2854..3210
                     /locus_tag="VIN75_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8974573.1"
                     /translation="MVQYSSRLDASFAALSDATRRGVIEQLWRGDASITELAERFHMT
                     LTGMKKHVGVLEQAGLVTTEKVGRVRTCRLGQGGLDEEWAWLGHYRSVWAARFDALDA
                     VVEELKRKERNDGRKK"
     gene            3194..3670
                     /locus_tag="VIN75_10185"
     CDS             3194..3670
                     /locus_tag="VIN75_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8974574.1"
                     /translation="MVEKSRHPTTVERKSDREVVVRRIFDAPARIVWDAWTRAEVFRQ
                     WWVPRSSGLKLQSFEQDVRVGGSYRVVFDLGDSRTMAFFGKYLEVVPASRLAWTNEES
                     EDGAVTTVTFEEKDGKTHLTLSERYASKEALDRNIGSTEGLPEQFEQLDDLLPTLG"
BASE COUNT          568 a         1248 c         1319 g          604 t
ORIGIN      
        1 cgccgcgcct cgatcgggct gcggaacttc gtgacgacgc cgtccagctg gatctcgccg
       61 gagtcgggca ccgtcgcgcc cgacagcgcc ttgatcagcg aggacttgcc ggcgccgttg
      121 tcgccgatga cggccaggat ctcgccggag cgcagctcga agtcgctgcc gtccagcgcg
      181 gtgaccgagc cgtagcgctt gaccagagac ttggcctgca tcacgacgcg gggttgcgtg
      241 gtcgcgctca tcgtgcacct cgacgcgaaa gttgatcggc cgtcaccgcg aggatgacca
      301 gcacgccggt gacgagcacc tggtagatcg acgacacgcc catcagcgtc aggccgttgc
      361 ggaacacgcc caccaccagc gcgcccacga gcgagccgat gacgacgccg cgcccaccga
      421 acaggctggt gccgcccagc acgacggccg tgatggcgtc caggttgtcg gtctgtcccg
      481 cgttcgggtc gccgacgccg gtgcgcgcca cgctcagcag cgacgcgacg ccgtacagcg
      541 cgccggccac cacgtagacg atcagcagca cgcgctgcgt cgcgatgccc acgagccgcg
      601 tggcctccgg gttgttgccc accgcgtaga cgtggcggcc agccgcggtc tcgcgcagca
      661 tgaaccacgc gatcgcgtac agcgccagca tcacgacggt gccccaggcg aactgcgtcg
      721 agccgatgcc gaaggtgtcg cccagcgtgg tcatcatcgg cgggaggtcg gtgacggtct
      781 gcgcgccgga gtacagctgc gtggccgcga acgcgatgtt catcgtgccc agcgtgacga
      841 tgaacgacgg caggcggatg cgcgtgacca gcagcccgtt gaccagcccg aacagcgtgg
      901 tcacgcccac gccgcacagg atggccagcg gcggcgccat gccgttgacg gccgcgaagc
      961 gcgtcatcac gatgccgccc agcgccatca gcatgccgca ggacaggtcg atgccgccgg
     1021 tcaggatcac cagcgtctgg ccgatggcga tgacgcccac cacggccacc tgctgcagga
     1081 tcagcgagaa gttggcgccg gacaggaagt ggtcgctctg ggtcgagaag aacacgcagg
     1141 cgagcaccag cgcgatcacg gggcccagca cggccacgct caggcccgcg ggccagtgcg
     1201 gccccgggtt gttcggcttg cccggagccg cgtcggaggt gctcacggcg gccatgtcac
     1261 ttgccccagc agagggattc gccgaccttg gagtccttgc tctcgacgcc cggcatggcc
     1321 ttgtcggcga tcagcgtgac gcccgtgtcg gtgtagccgc tgaccttggt gcccttggtg
     1381 gcgtagtcga cgcccgcggc gacgccgagc gcggccatct tcagcgggta ttgctgcgac
     1441 gtggcggcga tgacgccggc cttgacgttg cggatgcccg cgcagccgcc gtccacggag
     1501 acgatgatca cgcccttttc cttgccggcg cgcttgagcg ccgtgtacgc gcccgcggcg
     1561 gccggctcgt tgatcgtgta gacgaggttg acgtcggggt tcttctgcag gcagttctcc
     1621 atcgccgtct ggcccttggc ctggtcgccg tagctgtcgg ccgagcagac gatctcggcc
     1681 gacttgccca gctcgttgac cttggcgtcc ggcgcggcca ggccgaagcc cttcatgaag
     1741 ccgttgtggc gctgtgcgcc gaccgggtgg ccggggaaca ggtcgagcgt gacgatcttc
     1801 gcgggcttgc cgccgagcgc cgccttggcg tactcgccga tcaggatgcc ggccttgtag
     1861 ttgtcggtgg cgaacagggc gtcggtggcg tcctgcggat cggtggggct gtccagagcg
     1921 atgaccatca cgcccttggc gcgcgccttc tggatggcgg gcacgatggc cttcgaatcg
     1981 ttggccgtga tcaggatcgt cttggcgccg gccgtcatca tgttctcgat ggccgtgacc
     2041 tgggcggcgt tgtcgccgtc ctgcttgccg gcggccgtca gcagccgggc gccgcgttcc
     2101 ttggccatct gcgtcgcgcc ttccttcatc ttcacgaaga acgggttggt gtcggtcttc
     2161 gtgatcaggc cgatcacggg ctggtcggcg gccagcgcca gggggctggc cgtcgcgagg
     2221 acggcggtcg cgagcaggga cgaggcgatc ttgagcatgg tgtctccggt ggttgatgaa
     2281 cggccgcttc tcgtgtcggc gtcgggtgag cgcatcgtaa gaaccctgga taaataaatc
     2341 aagcggactg attaatggaa aacccagggt cgattgccgc ggcccggcgg ggctgcctgg
     2401 acgacaatcg ccgcatgctc ctgcgccttc atcacgccgc catcatctgt tccgactacg
     2461 aggcctccaa gcgcttctac acgcaggtgc tgggcctgcg cgtgatcgcc gagcactggc
     2521 gcgcggcgcg gcgttcctgg aagctggacc tgggcctgcc cgacggttcg caggtcgagc
     2581 tgttctcgtt cgagggcgcg ccgccgcggc cgtcgtaccc ggaggcgcgc ggcctgcggc
     2641 acctggcgtt cgcggtggca gacgtcgccg ccgtgaaggc gcatctccaa gcgcacggcg
     2701 tcgcggtcga ggacatccgg gtcgacgagt acaccggccg ccgcttcacc ttcttcgccg
     2761 atccggacga actgccgctg gaggtctacg aagaccctgc ctgaatcgat ggcgcgcttg
     2821 cgtccgtccc ttcacgcgct tatagtgaac tcaatggttc agtattcgtc acgcctcgat
     2881 gcctccttcg ccgcgctgtc cgacgcgacg cggcgcggcg tcatcgagca gctctggcgc
     2941 ggcgacgcgt ccatcaccga gctggccgag cgcttccaca tgacgctgac cggcatgaag
     3001 aagcacgtcg gcgtgctgga gcaggcgggg ctggtcacca ccgagaaggt cgggcgcgtg
     3061 cgcacctgcc ggctcggcca gggcggcctg gacgaggagt gggcctggct cggccactac
     3121 cgcagcgtct gggcggcgcg cttcgatgcg ctggacgcgg ttgtcgagga actcaagcgc
     3181 aaggagagaa acgatggtcg aaaaaagtag gcacccgacg actgtggagc gcaagtccga
     3241 tcgcgaagtg gtcgtcaggc gcatcttcga cgcgcccgcg cgcatcgtct gggacgcctg
     3301 gacgcgggcc gaggtgttcc ggcagtggtg ggtgcccagg tccagcggcc tgaagctgca
     3361 gtccttcgag caggacgtgc gcgtcggcgg cagctaccgc gtggtgttcg acctgggcga
     3421 ttcgaggacc atggccttct tcggcaagta cctcgaggtg gtgcccgcct cgcgcctggc
     3481 ctggacgaac gaggagagcg aggacggcgc cgtgaccacc gtgaccttcg aggagaagga
     3541 cggcaagacg cacctgacgc tgtccgaacg ctatgccagc aaggaagcgc tcgaccgcaa
     3601 catcggaagc acggaagggc tgccggagca gttcgagcag ctggacgacc tgctgccgac
     3661 gctgggttag cctgcgggcg ctccgcgtcg cggcgctcgc gccgctcgag gggaccggcc
     3721 tcggactcag cccctcatg
//
LOCUS       DATCRO010000375         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1050122, whole genome shotgun sequence.
ACCESSION   DATCRO010000375 DATCRO010000000
VERSION     DATCRO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050122"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VIN75_10190"
     CDS             <1..1073
                     /locus_tag="VIN75_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974575.1"
                     /translation="PGPDKFSLSLQGPDPDLAVGGTHDQQEAQRDETVKLEAYAGSSY
                     ETRLLPGSLRWPSDGDVQGILSGLKPNPPIGVDGKDVTHAVYQSEIPNATPDQVFAHW
                     TLQPNEVFNAGGMEIRPPIKTLQDGRYMLETGGTNAPPTWLPVQIKVDPAARTIHIDT
                     LDGHVLRGEQTFYFKDDGCGGTRIVQDARFQASSQLTGDMQHFLPISQGQHDAWQQAH
                     LETYEQFNGDPGYKGIGVPYVDPLTQGEALGDKAFLQVVAHPARAADAAISALGDGAN
                     YGIDLSGRLTADALDGAGDTTRQTLDWLHLPGGAAIDSASHETGQFISSLADKTGDVV
                     ESGMHKVGDATKSVLDALNPFG"
     gene            complement(1077..>2996)
                     /locus_tag="VIN75_10195"
     CDS             complement(1077..>2996)
                     /locus_tag="VIN75_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8974576.1"
                     /translation="DGVVKLVDFGIAKRIGPGAAEPVTRTATAQRAMTLVYSSPEQVR
                     GGEITPASDIYSLGVVLYRLLTNTSPYPAAATDSPYELTRAICDTEPVPPSAAASSEP
                     ESVRRRLRCDLDAVVMMALRKDPARRYASAEAMSEDLFRHLEGLPVQARRGAWSYRAG
                     RFMLRHRGAVVLVLVANLALVAGLGSAAYEGVQARRERVLAQRHFESVRGLAHAFIFD
                     VHDAIARLPGSIDARRKLVDTALVYLKQLGDESQNDPELQLEIASGYDHIGEIQGGGT
                     VASLGDAKAGLASFDRALAIVRPLMARPGPARRDAAKEFVTLSAHEGSVLMTQGRWHD
                     AEALLREGMGVAQARVDKAPDDYDALKQLASNSGLLAMMYLRSANRDAFDAMSRQHVA
                     QEEHLHALRPDDVDVVADLGTAHTARAVNLMQNVGTPEARREAVGEYQRALDIMEPAY
                     ARNPLHWILASNYGRTQGYFGHLLTQMGQSKDALVHLRRAVDVADAMAGKDPREVRAR
                     TEQAEAHGRLGEALLQLHDAAGGTEQAEQAWAAFSALPQQTQDEVIIQFNRAATQSLL
                     GRALEARAGQEAGAAARQDGVAGCQHLRAAQALLAVNLPRRASSPMTESMKQSVDADV
                     AHCRATADGAGVKQP"
BASE COUNT          436 a         1081 c         1056 g          423 t
ORIGIN      
        1 acccgggccc cgacaagttc agcctctcgc tgcaagggcc ggatccggac ctcgccgtcg
       61 gcggcacgca cgaccagcag gaggcgcagc gcgacgagac ggtgaagctc gaggcctatg
      121 ccgggtccag ctacgagacc cgcctgctgc ccggcagcct gcgctggccg agcgatggcg
      181 acgtgcaggg catcctgtcc ggcctgaagc cgaacccgcc gatcggcgtc gacggcaagg
      241 acgtcacgca cgcggtctac cagagcgaga tccccaacgc cacgccggac caggtcttcg
      301 cccactggac gctgcagccc aacgaggtct tcaacgccgg cggcatggag atccggccgc
      361 cgatcaagac gctgcaggac ggccgctaca tgctcgagac cggcggcacc aacgcgccgc
      421 cgacctggct gccggtccag atcaaggtcg acccggctgc acgcacgatc cacatcgaca
      481 cgctggacgg ccacgtgctg cgcggcgagc agacgttcta tttcaaggac gacggctgcg
      541 gcggcacgcg catcgtgcag gacgcgcgct tccaggcgag ttcgcagctc accggcgaca
      601 tgcagcactt cctgccgatc tcgcagggcc agcacgacgc ctggcagcag gcgcacctgg
      661 agacctacga gcagttcaac ggcgatcccg gctacaaggg gatcggcgtt ccctacgtcg
      721 atccgctgac gcaaggcgag gccctgggcg acaaggcctt cctgcaagtc gtggcccatc
      781 ccgctcgcgc cgcggacgcg gccatcagcg cgctcggcga cggcgccaac tatgggatcg
      841 acctgagcgg ccggctcacg gccgacgcgc tcgacggcgc cggcgacacc acgcgccaga
      901 cgctcgactg gctgcacctg ccgggcggcg ccgccatcga cagtgcctcc cacgagacgg
      961 ggcaattcat ctcgagcctg gcggacaaga ccggcgacgt cgtggagtcc ggcatgcaca
     1021 aggtcggcga cgccacgaag tcggtcctcg acgcgctgaa tccgttcggt taggcgtcaa
     1081 ggctgcttga cgccggcacc gtcggccgtc gcccggcagt gcgcgacatc ggcgtcgacc
     1141 gactgcttca tcgattcggt catcggactg gaggcccggc gcggcaagtt cacggccagc
     1201 agcgcctggg cggcgcgcag gtgctggcag ccggccacac cgtcctgcct ggccgccgcg
     1261 cccgcctcct ggccggcgcg cgcctcgagc gcgcgtccga gcagcgactg cgttgccgcc
     1321 cggttgaact ggatgatcac ctcgtcctgg gtctgctggg gcaacgcgct gaaggccgcc
     1381 caggcctgct cggcctgctc ggtgccgccg gccgcgtcgt gcaactgcag cagcgcctcg
     1441 cccagccggc catgggcctc cgcctgctcg gtgcgcgcgc gcacctcgcg ggggtccttg
     1501 ccggccatcg cgtcggccac gtccacggcg cggcgcaggt gcaccagggc gtccttggac
     1561 tgccccatct gggtgagcag atggccgaag tagccctggg tgcgtccgta gttgctggcc
     1621 aggatccagt gcagcggatt gcgcgcgtag gcgggctcca tgatgtccag cgcgcgctgg
     1681 tattcgccga ccgcctcgcg cctggcctcg ggcgtgccga cgttctgcat caggttgacc
     1741 gcgcgcgccg tgtgcgccgt gcccaggtcg gcgacgacgt cgacatcgtc cggccgaagc
     1801 gcgtgaagat gctcttcctg cgcgacatgc tgcctggaca tcgcgtcgaa tgcgtcgcgg
     1861 ttggccgagc gcaggtacat catggccagc agccccgaat tggacgcgag ctgcttgagc
     1921 gcatcgtagt cgtcgggcgc cttgtccacg cgcgcctggg ccacccccat gccttcgcgc
     1981 agcaaggctt ccgcgtcgtg ccatcggccc tgggtcatga gcacggaacc ttcgtgggcg
     2041 ctgagcgtca cgaactcctt ggcggcatcg cgccgggccg gacccggcct ggccatgagc
     2101 ggccgcacga tcgcgagcgc gcgatcgaag ctggccaggc ccgccttggc gtcgccgagg
     2161 ctggcgaccg tgccgccgcc ctggatctcg ccgatgtggt cgtagcccga ggcgatctcc
     2221 agttgcaact cgggatcgtt ctgcgactcg tctccgagct gcttcaggta cacgagcgcg
     2281 gtgtcgacga gcttgcggcg cgcgtcgatc gagcccggca gccgggcgat ggcgtcgtgc
     2341 acgtcgaaga tgaacgcgtg ggccaggccg cgcacgctct cgaagtggcg ctgggcgagg
     2401 acgcgttcgc ggcgggcctg cacgccttcg taggcggccg atccgaggcc ggcgacgagc
     2461 gccaggttgg cgaccagcac cagcaccacc gcgccgcgat ggcgcagcat gaagcgcccc
     2521 gcgcggtagc tccaggcgcc gcgccgcgcc tgcacgggca ggccttcgag gtgacggaac
     2581 aggtcctcgg acatcgcctc ggccgacgca tagcggcgcg cgggatcctt gcgcagcgcc
     2641 atcatcacga cggcgtcgag gtcgcagcgc aggcgccgcc gcacgctctc gggctcgctc
     2701 gacgcggcgg cactgggcgg cacgggttcg gtgtcgcaga tggcccgcgt gagctcgtag
     2761 gggctgtcgg tggccgcggc cgggtacggg ctcgtgttgg tcagcaggcg atacaggacc
     2821 acgccgagcg agtagatgtc gctggccggc gtgatctcgc cgccgcgcac ctgctcgggg
     2881 ctggagtaca ccagcgtcat cgcgcgctgc gcggtcgccg tgcgcgtgac gggctcggcc
     2941 gcgccgggcc cgatgcgctt ggcgatgccg aagtccacca gcttgaccac gccgtc
//
LOCUS       DATCRO010000376         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_408947, whole genome shotgun sequence.
ACCESSION   DATCRO010000376 DATCRO010000000
VERSION     DATCRO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408947"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VIN75_10200"
     CDS             <1..622
                     /locus_tag="VIN75_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY8974577.1"
                     /translation="DAEFRELMCVELRKLHERLKTTTVFVTHDQNEAMALADHIVVMN
                     QGEILQSDTPEGVYNFPSCLFVARFIGRPAMNLLPLDERVMADWDTVRVAGTDITVPT
                     PLASVQRALLGVRPEHVSLRPAGEGIAAQVAQVEYFGSHWVASLATAAGNVCALVSKE
                     TAPAVGDAVGITFETRRTVLFDASTEQLIPSATTLAHRAGKAPCQA"
     gene            610..1620
                     /locus_tag="VIN75_10205"
     CDS             610..1620
                     /locus_tag="VIN75_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974578.1"
                     /translation="MSSLTLQSATKRFGAVTAVADLDLDVHDGEFFVLLGPSGAGKTT
                     TLRLAAGLETPDQGRVLMDGQDVTHLPPAPRDVAFVFQQYSLYPHLTVFDNIAFPLRS
                     AMRRVDAGELKRRVERVAETLHLENKLQRKATALSGGEMQRVAIGRALVREPRVFLMD
                     EPLSSLDAKLREELRAELKALQRRTGATVLYVTHDQVEATTLADRIAILEDGRVKQVG
                     TPREIYDQPRSLHVAQRLGSPAFNVLPVSWFEGSVDPRTVHVGIRPEDVELAAGDGPS
                     HFDVIECSLVKHLIVAERGGVEVRIRRVMDRAFAPGDRVRVSFPLKNCLRFDADGRRL
                     DT"
     gene            1636..>2261
                     /gene="dhaL"
                     /locus_tag="VIN75_10210"
     CDS             1636..>2261
                     /gene="dhaL"
                     /locus_tag="VIN75_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY8974579.1"
                     /translation="MNTMLIIRSARAVQQSILSHEADIEMLDRAIGDGDHFINVRRGC
                     EVVAALAPELEPLAPSEALQKIGMKLLGTIGGASGPLIASFFLAMGKTLDGIVEPERH
                     DFAAAFAAGVEAIKLRGKADVGEKTMLDVLIPAARMLTRLVDEDVALSDICAQMSDEA
                     HQNMLATKDMIATKGRAYFLGERALGHIDPGCKTCQVAIEAVCDVVLQP"
BASE COUNT          350 a          815 c          772 g          324 t
ORIGIN      
        1 ggacgccgag ttccgcgagc tgatgtgcgt ggagctgcgc aagctgcacg agcggctgaa
       61 gacgaccacc gtgttcgtca cgcacgacca gaacgaggcg atggcgctgg ccgaccacat
      121 cgtcgtcatg aaccagggcg agatcctgca gtcggacacg cccgagggcg tctacaactt
      181 cccgtcctgc ctgttcgtgg cccgcttcat cggccgcccg gccatgaacc tgctgccgct
      241 ggacgagcgc gtgatggcgg actgggacac cgtgcgcgtg gccggcaccg acatcaccgt
      301 gccgacgccg ctggcgagcg tgcagcgcgc gctgctgggc gtgcgtcccg agcacgtgag
      361 cctgcggccg gcgggcgagg gcatcgccgc gcaggtcgcg caggtcgagt acttcggctc
      421 gcactgggtc gcgagcctgg ccaccgccgc cggcaacgtg tgcgcgctgg tgagcaagga
      481 gaccgccccg gccgtcggcg atgccgtcgg catcacgttc gagacccggc gcaccgtcct
      541 gttcgacgcg agcaccgagc aactcatccc gagcgccacg acgctcgccc accgcgcggg
      601 aaaagcacca tgtcaagcct gacgctgcag tccgccacca agcgcttcgg cgccgtcacg
      661 gccgtcgcgg acctcgacct cgacgtgcac gacggcgagt tcttcgtcct gctgggcccg
      721 agcggcgccg gcaagaccac cacgctgcgc ctggcggccg gcctcgagac gcccgaccag
      781 ggccgcgtgc tgatggacgg ccaggacgtc acgcacctgc cgcctgcgcc gcgcgacgtc
      841 gccttcgtgt tccagcagta ttcgctgtac ccgcacctga cggtgttcga caacatcgcg
      901 ttcccgctgc gctcggcgat gcggcgcgtg gacgccggcg agctcaagcg gcgcgtcgag
      961 cgcgtcgccg agacgctgca cctggaaaac aagctccagc gcaaggcgac ggcgctgtcc
     1021 ggcggcgaga tgcagcgcgt ggccatcggc cgcgccctcg tgcgcgagcc gcgcgtcttc
     1081 ctgatggacg agccgctgtc gtcgctcgac gccaagctgc gcgaggaact gcgcgccgag
     1141 ctcaaggccc tgcagcgccg caccggcgcg accgtgctgt acgtgacgca cgaccaggtg
     1201 gaggccacga cgctggccga tcgcatcgcc atcctggagg acggtcgcgt caagcaggtc
     1261 ggcacgccgc gcgagatcta cgaccagccg cgcagcctgc acgtcgcgca gcgcctcggt
     1321 tcgcctgcgt tcaacgtgct gccggtcagc tggttcgagg gcagcgtcga tccgcgcacc
     1381 gtccatgtgg gcatccgccc cgaggacgtg gaactcgcgg ccggtgacgg gccgtcgcac
     1441 ttcgacgtca tcgaatgctc gctcgtgaag cacctcatcg tcgccgagcg cggcggcgtc
     1501 gaggtgcgga tccgccgcgt gatggatcgc gccttcgcgc ccggcgaccg cgtccgcgtc
     1561 tcgtttccgc tcaagaactg cctgcgcttc gatgccgatg gccgacgcct ggatacctga
     1621 agggagtacc tcaccatgaa caccatgctc atcatccggt cggcgcgcgc cgtccagcag
     1681 tcgatcctgt cgcacgaggc cgacatcgag atgctcgatc gcgcgatcgg cgacggcgac
     1741 catttcatca acgtgcgccg cggctgcgag gtcgtggccg ccctcgcccc cgagctcgag
     1801 ccgctggcgc cttccgaggc gctgcagaag atcggcatga agctgctggg caccatcggc
     1861 ggcgcctcgg ggccgctgat cgcgagcttc ttcctggcca tgggaaagac gctcgacggc
     1921 atcgtcgaac ccgagcggca cgacttcgcc gcggccttcg ccgcgggcgt cgaggcgatc
     1981 aagctgcgcg gcaaggccga cgtcggcgag aagacgatgc tcgacgtcct catcccggcg
     2041 gcgcggatgc tgacgcggct cgtcgacgag gacgtggccc tgtccgacat ctgcgcgcag
     2101 atgtccgacg aggcccacca gaacatgctg gcgaccaagg acatgatcgc caccaagggg
     2161 cgcgcgtact tcctgggcga gcgcgcgctc ggccacatcg accccggatg caagacctgc
     2221 caggtcgcga tcgaggcggt gtgcgacgtg gtcttgcaac c
//
LOCUS       DATCRO010000377         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_656795, whole genome shotgun sequence.
ACCESSION   DATCRO010000377 DATCRO010000000
VERSION     DATCRO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656795"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VIN75_10215"
     CDS             complement(<1..375)
                     /locus_tag="VIN75_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8974580.1"
                     /translation="MFSDLIFRQLFDAASSTYTYLLGCPETRQAVLVDAVFEQHLRDR
                     ALVEELGLTLVATLDTHCHADHVTGAWLMQQATGCRIGVSARYGGELRGADLRLDHGD
                     RVAFGRRHLDVRATPGHTDGCLT"
     gene            complement(418..1074)
                     /locus_tag="VIN75_10220"
     CDS             complement(418..1074)
                     /locus_tag="VIN75_10220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8974581.1"
                     /translation="MSLRINDTAPNFTAQTTHGTINFHDWIGDSWAVLFSHPKDFTPV
                     CTTELGYMARIEPEFTKRNTKIIGLSIDPVDSHSNWEADIEETQGAKVKYPMIGDTDL
                     AVAKLYNMLPADEAGGSDGRTAATNATVRSVFVIGPDKKIKLMLTYPMTTGRNFDEIL
                     RVLDSMQLTAQHKVATPVNWKKGEDVIITTAVSNEEAAVKFPGYKTIKPYLRTTRQPS
                     "
     gene            complement(1239..1589)
                     /locus_tag="VIN75_10225"
     CDS             complement(1239..1589)
                     /locus_tag="VIN75_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974582.1"
                     /translation="MRPVVEEQKARDGSFDGVMPATWSASGANVVISRSLNFAVSHRT
                     RTWVPIANYGKAHFVIENEDCHVVASGSVTPFIVPRVNFYVDEGPATPPAPAPAARTL
                     ARKAAAKPAHGVQP"
     gene            complement(1622..2182)
                     /locus_tag="VIN75_10230"
     CDS             complement(1622..2182)
                     /locus_tag="VIN75_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974583.1"
                     /translation="MIAPRTSTLRRTLAVAAGALALACGAAQAAPGWHGGGGYHGGGG
                     YHGGGGWHGDGHWHSGYRWGYRSGWGWGWWDPLWPVGVGLGIGLATAPSYYYGPDYVV
                     VNPAPQYYTPAPVYVNPPAPAAAPAPRSADPIFYPRNGQSAAQTEADRQDCNRWATTQ
                     PNAMNDASVFQRATFACMDGRGYTVR"
     gene            complement(2422..>2612)
                     /locus_tag="VIN75_10235"
     CDS             complement(2422..>2612)
                     /locus_tag="VIN75_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY8974584.1"
                     /translation="DDASLLGLPRMRWLDALVFSSPGRPWRDVMVAGRWALRGHHHAQ
                     VERISAGFARAMGELWGE"
BASE COUNT          357 a          841 c         1017 g          397 t
ORIGIN      
        1 ggtcaggcag ccgtcggtgt ggcccggcgt ggcgcgcacg tcgaggtggc gccggccgaa
       61 ggcgacgcgg tcgccgtggt ccagccgcag gtcggcgccg cgcagctcgc cgccgtagcg
      121 tgccgacacg ccgatgcggc agcccgtggc ctgctgcatc agccaggcgc cggtgacgtg
      181 gtcggcgtgg cagtgggtat cgagcgtggc caccagcgtc agcccgagct cctcgaccag
      241 cgcgcgatcg cgcaggtgct gctcgaacac cgcgtcgacc agcacggcct gccgcgtctc
      301 ggggcagccg agcaggtagg tgtaggtcga cgacgcggcg tcgaacagct ggcggaagat
      361 gaggtcgctg aacatgggca tgcctgggtc gcggcccgcg cgcgggccgc atcgggctca
      421 ggacggctgc cgcgtggtgc gcaggtaggg cttgatcgtc ttgtagccgg ggaacttgac
      481 ggcggcctcc tcgttggaca ccgccgtggt gatgatgacg tcctcgccct tcttccagtt
      541 caccggcgtg gccaccttgt gctgggcggt cagctgcatc gaatcgagca cgcgcaggat
      601 ctcgtcgaaa ttgcggcccg tggtcatcgg gtaggtcagc atcagcttga tcttcttgtc
      661 cgggccgatg acgaacaccg agcgcacggt ggcgttggtg gccgcggtgc ggccgtccga
      721 gccgccggcc tcgtcggcgg gcagcatgtt gtagagcttg gccaccgcga ggtcggtgtc
      781 gccgatcatc gggtacttga ccttggcgcc ctgcgtctcc tcgatgtcgg cttcccagtt
      841 cgagtggctg tcgaccgggt cgatcgacag gccgatgatc ttggtgttgc gcttggtgaa
      901 ctcgggctcg atccgggcca tgtagcccag ctcggtggtg cacaccggcg tgaagtcctt
      961 cgggtgcgag aacaggacgg cccagctgtc gccgatccag tcgtggaagt tgatggtgcc
     1021 gtgggtggtc tgggcggtga agttgggggc ggtgtcgttg atgcgaaggg acatggcagg
     1081 ctccggctgg gttgcggggg aacgggtgca gtgtcgcgca gtggcgtgac ccgttccacg
     1141 aggaaggtta ccgaagccga ccggtgaaaa cccgcatgac gatctgcgcg gcgtgcacgg
     1201 tcggctgggg tgggccgcgc ggcgcggccc ggccgggctc agggctgcac gccgtgcgcc
     1261 ggcttggccg ccgccttgcg ggccagcgtc ctggcggccg gcgccggagc gggcggcgtc
     1321 gccggacctt cgtcgacgta gaagttgacg cgcggcacga tgaacggcgt gaccgacccg
     1381 ctggccacga cgtggcagtc ctcgttctcg atcacgaagt gcgccttgcc gtagttggcg
     1441 atcggcaccc aggtgcgcgt gcggtgcgac accgcgaagt tgagcgagcg cgagatcacc
     1501 acgttggcgc cgctggcgct ccaggtggcc ggcatcacgc cgtcgaacga gccgtcgcgc
     1561 gccttctgct cctcgacgac aggccgcatc gagcggcctg ccttcgaggg gacgaggggg
     1621 gtcagcgcac ggtgtagccg cggccgtcca tgcacgcgaa cgtggcgcgc tggaacacgc
     1681 tggcgtcgtt catcgcgttg ggctgcgtgg tggcccagcg attgcagtcc tggcgatcgg
     1741 cctcggtctg cgcggcgctc tggccgttgc gcgggtagaa gatcgggtcg gcgctgcgcg
     1801 gagccggcgc cgcggcgggc gccggcgggt tcacgtagac cggggcaggc gtgtagtact
     1861 ggggcgccgg gttgacgacg acgtagtcgg ggccgtagta gtacgacggc gcggtggcca
     1921 ggccgatgcc caggccgacg ccgacgggcc acagcggatc ccaccagccc cagccccagc
     1981 cggagcggta gccccagcgg tagccggagt gccaatggcc gtcgccgtgc cagccgccgc
     2041 cgccgtggta tccgccgccg ccgtgatagc caccgccgcc gtgccagccg ggggcggcct
     2101 gggcggcgcc gcaggcgagc gccagcgcac cggcggcgac ggccagcgtg cggcggaggg
     2161 tggaagtgcg gggggcgatc atgacggggc tccttgtggc tacgcgcgaa gcgcgttcaa
     2221 tgattctctg cctttaacgg caggtcgccg gcgtgggagg acggccttta cacgccagat
     2281 acccgggtgg gatccggcgt gtgcgcacgg gttccgcgag gggatcggac gatcagtcga
     2341 aagcgcgcca aaaggggccg attgcgcgcg ggttgtcgcc agaagggcga cgaacgtgtc
     2401 cgcggccgtg gagggcctcc gtcactcgcc ccagagctcg cccatcgccc gagcaaagcc
     2461 cgccgagatg cgctcgacct gcgcatggtg gtgcccgcgc aacgcccagc gaccggccac
     2521 catgacgtcg cgccagggcc ggccggggct ggagaagacg agcgcgtcga gccagcgcat
     2581 gcgcggcagg cccagcaggc tggcgtcgtc gg
//
LOCUS       DATCRO010000378         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_712865, whole genome shotgun sequence.
ACCESSION   DATCRO010000378 DATCRO010000000
VERSION     DATCRO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712865"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1792)
                     /gene="malQ"
                     /locus_tag="VIN75_10240"
     CDS             complement(269..1792)
                     /gene="malQ"
                     /locus_tag="VIN75_10240"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY8974585.1"
                     /translation="MTIDLHKRSAGVLLHTTSLPGPHGIGDFGPAAFQFVDWLVAAGQ
                     SIWQLLPTAPIGPGNSPYQSVSAFAGSPLMVALEPLVAKGWIAEPALPDGGFDPHFVD
                     YGKVIPWRIAQLRAAARGFAEKADKSERDACAAWCAKHASWLDDYALFMALETTSGYK
                     PWWEWDGGLQRRTQSALAAARTQHAGEIAFWQFVQWQFDLQLSAIKKYANDRGVFIMG
                     DLPIFVAHHSADVWARPDLYTLDENFQPTVVAGCPPDAMAVDGQRWGNPLYHWDRMAK
                     EDFAWWTARVRRALEQADVFRIDHFRGFAGYYEIPASCPTAREGTWRTGPGRPLFDAI
                     AKALGPLPIVAEDLGFITQDVYDLRDGLGYPGMRILQFAFGGDASDTFLPHNYTRYTI
                     AYTGTHDNDTARGWWDNATPHERAYVGSYLASGGDDIHWAMIRACCNSVANVAVFQLQ
                     DVLGLGSAHRMNTPGTVGDRNWSWRFDWSMLGSEPGRVLGLISAASGRAPIGLVHLP"
     gene            complement(1789..3909)
                     /gene="glgX"
                     /locus_tag="VIN75_10245"
     CDS             complement(1789..3909)
                     /gene="glgX"
                     /locus_tag="VIN75_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY8974586.1"
                     /translation="MSDLPATAQPGRCEPMGAHARDGGVNFAVFSQNAQRIELCLFDA
                     DGRAELRRYELHGPHDGVFHGFLPGVGVGQVYGLRAHGPYDPCNGHRFNAHKLLLDPC
                     AREIVGRFEWRPEHHGYQLGHPEGPQSFDQRDNAAIALKARVAPAGTPARGGSNAPRI
                     APADVVLYEVHVKGFSKQLGDVPPGLRGTYAGLAHPASIAHFKALGVTTLSLLPVQYH
                     VDEPALGGRGLTNYWGYNTLGFFCPDPRLSMTPDDPSAVNAEFRSMVETLHAHGLEVV
                     LDVVYNHTPEGNEAGPTISFRGLDNASWYRLMPDDAGRLENLTGCGNTVNVAHPQVTQ
                     FVLDSLRYWVQEMGVDGFRFDLAPVLGRTKQGFQPDAPFFVALRQDPVLSRVHLIAEP
                     WDAAPDGYQVGRFPGRWLEWNDKFRDTVRRYWLFAGTPQAVGRGELARRFTASSDLFN
                     HGQRTPLASVNFIAVHDGYTLADLTSFDRKHNEANGEGNRDGRDGEPSASFGVEGPTG
                     DPWILAQRGRVRRAMMATLLLAQGTPMLCAGDEIGNSQQGNNNAYCHDGPLTWLDWSA
                     ADTSFQRFVADVLALRRAHATLRPTDWRSTAAVGAGAPRLTWLSPRGHEMAAADWHDE
                     KAQSFACRIDPAPTAAPPLDPALLVIFNGEDRALDFRLPAGFWQLALDSSLELHAEIF
                     PDTVLTQSLHLPARSLVVIRQKTP"
BASE COUNT          579 a         1353 c         1507 g          576 t
ORIGIN      
        1 ggcgctgagc gcgcgccggg cgttgatgca ggtgtcgctg aaccggcaga cggccgccat
       61 caacatggtg tcggcgctgg gcggcgggtg gtcgacgcag aagttggcca aggattgacg
      121 cgtcgcggac cagggcgccc ccggaagcgg tctggcccat cctcgaatcg cgctcgcacg
      181 ttgccggcag ctaggagtct gcgcggcaga agccgcacag actcctaggt cgcctgtcgc
      241 gattcgaggc caggcctgac tccctttctc agggcaagtg caccagcccg atcggcgccc
      301 gaccgctcgc cgcgctgatc aggcccagca cgcggcccgg ctcgctgccc agcatggacc
      361 agtcgaagcg ccaggaccag ttccggtcgc ccaccgtgcc aggcgtgttc atccggtgcg
      421 cgctgcccag gcccaggacg tcctgcaact ggaacacggc gacattggcc accgagttgc
      481 agcaggcgcg gatcatcgcc cagtggatgt cgtcgccgcc gctggcgagg tagctgccga
      541 cataggcgcg ctcgtgcggg gtggcgttgt cccaccagcc gcgcgcggtg tcgttgtcgt
      601 gcgtgcccgt gtaggcgatg gtgtagcgcg tgtagttgtg cggcaggaag gtgtcgctcg
      661 cgtcgccgcc gaaggcgaac tgcaggatgc gcatgccggg gtagccgagg ccgtcgcgca
      721 ggtcgtacac gtcctgcgtg atgaatccga ggtcttcggc gacgatgggc agcgggccca
      781 gcgccttggc gatcgcgtcg aacagcggcc tgcccgggcc cgtgcgccag gtgccctcgc
      841 gcgccgtggg acacgacgcc gggatctcgt agtagccggc gaagccgcgg aagtggtcga
      901 tgcggaacac gtcggcctgc tccagcgcgc ggcgcacgcg ggccgtccac caggcgaagt
      961 cttccttggc catgcggtcc cagtggtaca gcggattgcc ccagcgctgg ccgtcgacgg
     1021 ccatcgcgtc gggcgggcag ccggccacga ccgtgggctg gaagttctcg tccagcgtgt
     1081 acaggtcggg ccgcgcccac acgtcggcgc tgtggtgcgc cacgaagatc ggcaggtcgc
     1141 ccatgatgaa gacgccgcgg tcgttcgcgt acttcttgat ggccgacagc tgcaggtcga
     1201 actgccactg cacgaactgc cagaacgcga tctcgccggc gtgctgcgtg cgcgcggcgg
     1261 cgagcgcgga ctgcgtgcgg cgctgcaggc cgccgtccca ctcccaccag ggcttgtagc
     1321 cggaggtcgt ctccagcgcc atgaacagcg cgtagtcgtc cagccatgac gcgtgcttcg
     1381 cgcaccaggc ggcgcaggcg tcgcgttcgg acttgtcggc cttctctgcg aagccccggg
     1441 ccgcggcacg cagctgcgcg atgcgccacg ggatgacctt gccgtagtcg acgaagtgcg
     1501 gatcgaagcc gccgtcgggc agcgccggct cggcgatcca gcccttggcc accagcggct
     1561 ccagcgccac catcagcggg ctgccggcga acgccgagac gctctggtac ggcgagttgc
     1621 ccgggccgat cggcgcggtc ggcagcaact gccagatcga ctggccggcg gccacgagcc
     1681 agtcgacgaa ctggaacgcg gcggggccga agtcgccgat gccgtgcggg ccgggcaagg
     1741 aggtggtgtg cagcagcacg ccggcggagc gcttgtggag atcgatggtc atggggtctt
     1801 ctgtctgatc accaccagcg aacgcgccgg caggtgcagg gattgggtga ggacggtgtc
     1861 ggggaagatc tcggcgtgca gctcgaggct ggagtcgagc gccagttgcc agaagcccgc
     1921 gggcaggcgg aagtcgagcg cgcgatcctc gccgttgaag atcacgagca gcgcgggatc
     1981 gagcggcggc gcggcggtgg gcgcggggtc gatgcggcag gcgaaggact gcgccttctc
     2041 gtcgtgccag tcggcggcgg ccatctcgtg gccgcgcggg ctgagccagg tcaggcgcgg
     2101 cgcgccggcg cccacggcgg cggtggagcg ccagtccgtc ggacggagcg tggcgtgcgc
     2161 gcgccgcagc gcgagcacgt cggcgacgaa gcgctggaac gaggtgtcgg ccgccgacca
     2221 gtccagccac gtcagcgggc cgtcgtggca gtacgcgttg ttgttgccct gctggctgtt
     2281 gccgatctcg tcgccggcgc acagcatcgg cgtgccctgc gcgagcagca gcgtggccat
     2341 catcgcgcgg cgcacgcggc cgcgctgggc gaggatccac gggtcgccgg tcgggccttc
     2401 cacgccgaag ctggcgctcg gctcgccgtc gcggccgtcg cgattgcctt cgccgttggc
     2461 ctcgttgtgc ttgcggtcga agctggtcag gtccgccagc gtgtagccgt cgtgcaccgc
     2521 gatgaagttg accgaggcga gcggcgtgcg ctggccgtgg ttgaacaggt cgctggacgc
     2581 cgtgaagcgg cgcgcgagct cgccgcgtcc cacggcctgg ggcgtgccgg cgaacagcca
     2641 gtagcggcgc acggtatcgc ggaacttgtc gttccattcg agccagcggc ccgggaagcg
     2701 gccgacctgg tagccgtccg gcgcggcgtc ccagggctcg gcgatcaggt gcacgcggga
     2761 caggacggga tcctggcgca gcgcgacgaa gaacggcgca tccggctgga agccctgctt
     2821 cgtgcgcccg agcaccggcg cgaggtcgaa gcggaagccg tcgacgccca tctcctgcac
     2881 ccagtagcgc agcgaatcga gcacgaactg cgtgacctgc gggtgcgcga cgttgacggt
     2941 gttgccacag ccggtgaggt tctccagccg gcccgcgtcg tcgggcatca ggcggtacca
     3001 gctcgcgttg tccaggccgc ggaagctgat cgtcggcccg gcctcgttgc cttcgggcgt
     3061 gtggttgtag acgacgtcca gcaccacttc gaggccgtgc gcgtgcagcg tctcgaccat
     3121 cgagcggaac tcggcgttga cggccgacgg atcgtccggc gtcatcgaca gccgcggatc
     3181 cgggcagaag aagcccagcg tgttgtagcc ccagtagttc gtcagcccgc ggccgccgag
     3241 cgccggctcg tcgacgtggt actgcaccgg cagcagcgac agcgtcgtca cgcccagcgc
     3301 cttgaagtgc gcgatggacg ccgggtgcgc gagtcccgca taggtgccgc gcagccccgg
     3361 cgggacgtcg cccagctgct tgctgaagcc cttgacgtgc acctcgtaca gcaccacgtc
     3421 ggccggcgcg atgcgcggcg cgttcgaccc gcctcgcgcg ggcgtgcccg cgggcgcgac
     3481 gcgggccttc agcgcgatgg ccgcgttgtc gcgctggtcg aaggactgcg gcccctcggg
     3541 atggcccagc tggtagccgt ggtgctcggg ccgccactcg aagcggccga cgatctcgcg
     3601 ggcgcacggg tcgagcagca gcttgtgcgc gttgaagcga tggccgttgc acgggtcgta
     3661 cgggccatgc gcacgcaggc cgtagacctg ccccaccccg acgcccggca ggaagccgtg
     3721 gaacacgccg tcgtgcggac catgcagctc gtagcgccgc agctcggcgc ggccgtcggc
     3781 gtcgaacagg cacagctcga tgcgctgcgc gttctgcgag aacaccgcga agttgacgcc
     3841 gccgtcgcgc gcatgggcgc ccatcggttc gcagcggccc ggctgcgcgg tggcgggaag
     3901 gtcggacatg ggaaagacgt cagccgggaa cgagatagat cgtggccagc ggcgggacga
     3961 ccagcatgat ggactgcgcg cgaccgtgcg acgccaccgg cgtcaccggc agcac
//
LOCUS       DATCRO010000379         7015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_870255, whole genome shotgun sequence.
ACCESSION   DATCRO010000379 DATCRO010000000
VERSION     DATCRO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870255"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..710)
                     /locus_tag="VIN75_10250"
     CDS             complement(408..710)
                     /locus_tag="VIN75_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974587.1"
                     /translation="MDEALLDFIKAEYFESVDSDVQYLRFLKSPTGVFDWRRSATAFR
                     LLYVGHLYHSLGMSAEEFAEFSRSIPRKSLESHALLFRARLTAEDFIDAVIKCAWL"
     gene            complement(1210..1455)
                     /locus_tag="VIN75_10255"
     CDS             complement(1210..1455)
                     /locus_tag="VIN75_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974588.1"
                     /translation="MVFVATTAAGLTEALRRADGDNAIWCGSDVITEIEYVQLRRPNL
                     SRFDYSLADLQLNDGALGTVEEHYPGQTIWVEAPRQL"
     gene            complement(2225..4066)
                     /locus_tag="VIN75_10260"
     CDS             complement(2225..4066)
                     /locus_tag="VIN75_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8974589.1"
                     /translation="MSATPHAKSPAAPATRHVAPRGRLELRQLLKWLRDDGALAHDEA
                     DKLLRRFGTSASGLHPLQRLGGASLVDIRDNRVLDTDALARWLAARFELPYARIDPLR
                     VDVGRVAEVMSVAYAESRRALPVTVNAKEVVIATCEPFDVAWVPEIEALVKRPVRLQL
                     AHPEDITRYTPEFYSLAASVRFANKTTGAAAANSFEQLVELGKNKDQLDANDQGVVQV
                     VDWLWQYAFEQRASDIHLEPRRDMGAIRLRIDGVLHTAQQVPPAVMNAMTARIKLLAR
                     MDVIERRRPQDGRIKTKRPDGAGGMPGAEVEMRLSTLPTAFGEKVVMRIFDPGTVDKA
                     FDALGFNPAEAERWQTLTTQAHGIILVTGPTGSGKTTTLYSTLKKLATEEVNVCTVED
                     PIEMIDAAFNQTQVQPALDLDFAAGLRSLMRQDPDIIMVGEIRDLETAEMAIQASLTG
                     HLVFSTLHTNDAPSAITRLVDLGVPPYLISATVIGVLAQRLARTLCASCKVPDESADT
                     VRMIEELAQPWKLGGRLRPMKAVGCPACRNTGYRGRAALYELMPITEPLKEAIHPTLD
                     VAKLSRQGVLEGMRPLRLAGLMKVAEGLTTLDEVLRCTPQWISGRAG"
     gene            complement(4077..4832)
                     /locus_tag="VIN75_10265"
     CDS             complement(4077..4832)
                     /locus_tag="VIN75_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY8974590.1"
                     /translation="MTRPRQTLEPATMSEQDGVRYLHLGTPWVQGAMRVRKPQAIELE
                     YVQRMMAWMLWRPQDEVDHGHAVQLGLGAASITRFTHKVMRMRTTAVELNPTVISACR
                     MWFQLPADDARLAVLEADAGAWVRDPANLQVADVLSVDLYDHDAAAPVLDSDAFYAAC
                     RGVLVHGGLMAVNLFGRDASFAASAARIASAFGADQVWSLRPTREGNTVVVAGRGVTV
                     PDRDTLVARADNIESRFGLPARKWLRMVRPLSL"
     gene            complement(4829..5338)
                     /locus_tag="VIN75_10270"
     CDS             complement(4829..5338)
                     /locus_tag="VIN75_10270"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEY8974591.1"
                     /translation="MLEGLAPVIDVDTRLVILGSFPGVASLAAQQYYGHPRNVLWRLL
                     STLWDEDLVALDYAARLDALRRHRVGLWDVYRRCEREGSLDSAIRAPEPNDLARLPAL
                     APGLRAVAHNGAESARSRRLTEQLGVDVHTLPSTSPANASWSFERKLAAWRDVFSRYD
                     LAPSTTAAP"
     gene            5473..5940
                     /locus_tag="VIN75_10275"
     CDS             5473..5940
                     /locus_tag="VIN75_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974592.1"
                     /translation="MARARRTILPWLAAAATVVAASAPAQDVHMRHLAKGMTTGKTPA
                     NAFMVIRTADDFARALNQRMNVEGHPAADARGTIDFDTEMAVGVLLSNRPTACAGVDI
                     TSVSKDGIVSVVHYRERRRKPHETCVATVTSPFDFVAIPKSGSPIRFQADGEP"
     gene            complement(5953..6297)
                     /locus_tag="VIN75_10280"
     CDS             complement(5953..6297)
                     /locus_tag="VIN75_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974593.1"
                     /translation="MNKLHVLLVTAALGLSVHAFAQDGADAARQQRMDEAYAKSHDTQ
                     VADSGVREDVHKAGHAVHEGVKATGHAVHTGVKATGHAVHQGVKATGHAIHEGVKATG
                     HAIHKGVDAVTK"
     gene            complement(6364..>7015)
                     /locus_tag="VIN75_10285"
     CDS             complement(6364..>7015)
                     /locus_tag="VIN75_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974594.1"
                     /translation="RHWNDQRSPTATVDDVRFLKAVIAEVIAQDHGDPSAVFMVGSSH
                     GGFMTMRFACDAGETLRAAASLLSTMPDALARNCKSPRPLPWLAVNGTEDPSVPFGGQ
                     VEGTVRRGEIQAGLRSADDTFRFWADRAGCAAPVAREAITDQAEDGRHRWAERIVRGH
                     CVGGQMSQQVVLHGSGHVIPGLVADNRLAERAVGPAAPEVDGGMLVWMHFKATLLK"
BASE COUNT         1110 a         2379 c         2417 g         1109 t
ORIGIN      
        1 atgctgactg gagtctccct ctcggaggga ggctggaggg tgcagtcaaa catgctggag
       61 ctccctctcg gagggggctc gggggagccg tgcattcaac cgctaggagt ctgcgcggct
      121 tctgccgcgc agactcctag gactgccccg agcgcgcccc acactcccaa acgtcgcgcg
      181 attcggtcaa ttcgcttgct gcggctcagt gccatgaacg cgtggtccgg cgataggaat
      241 agacggcgag agcagtctaa cgcccatgac tgcctctgac cgggatcggg cttgctcatc
      301 aaggcgctcc gcgttcctgc gttcgaacac gagtgaatcg aagtcgtagt ctgtgcgggt
      361 cgccgacggt gcttatccac ctacacggcg tcatgcattt ggacgtgtca aagccaagca
      421 cacttgatga cggcatcaat aaagtcctca gcggtcaatc tcgctctgaa tagaagagca
      481 tgactctcca gcgattttct aggtatcgaa cgagaaaatt ccgcgaattc ttcggcgctc
      541 attccgagcg agtggtacaa gtgtccgaca taaagcaatc taaaggcggt agctgaacgc
      601 ctccagtcaa agacaccggt tggactcttg agaaagcgca ggtattggac atctgaatca
      661 accgattcaa aatattctgc ctttataaag tcaagcaatg cttcgtccac catagccgcg
      721 tctatccgaa ccattgggtc ctgtacaaca gccggaaacg tgacgacgct tggccgccga
      781 tctatgccga aagggcgtgc ggcgatcgac gagtaaggcc caggagaaat cgcattagcc
      841 gtggtcaaca ctttcgaccg aaagccggcg tgttcgattc cgaaggatag tgtgaacccc
      901 tcaatgcgcg agtgcatttc gaagcaaaaa acgtcgctcc accgcccggc tcgagatctg
      961 acgtaccaga gatccgactc ctcctgcaag gcgagcgtga accctcgcgc gtgcataaca
     1021 ctgctgatga tgttcctaag tgccataaat caatggcctg gcggcctcgc gtatgaccca
     1081 atcgactctc attgcgggcc gcagtcagtg ggcaagcaaa gcggcacctt cgaattgagc
     1141 ttccctgtgc cccttccggg gcgctggcgg gaggcgacca agttgccggg caggtgcctc
     1201 tcgtgctgat cacagttgcc gaggagcttc cacccagatg gtctgtcccg gatagtgttc
     1261 ttcaaccgta ccaagcgcgc cgtcgttcaa ttgcaagtcg gcaagactgt agtcgaaccg
     1321 actgaggttc ggacgccgaa gctgcacgta ctcgatctcg gtgatcacgt cgctgccgca
     1381 ccagatcgca ttgtcgccgt cggctcggcg gagagcttcg gtaaggccag cagcggtggt
     1441 cgcaacaaat accatcgaag ttcctcctag ggtaaagcct gcagccagcc tgctcgaaag
     1501 cggacgcaag tcgaggacgg ctcatggcca aggctgggtg aaaactcggc acacgagcaa
     1561 tttcgggggt cacttgaccc ctaccgaggt cgcgatcgtt caactatggg cgttctgacg
     1621 cgtcgacttt tcgtcttgca gaggcggcgc cgagttttca cacagcctcg gccggtagca
     1681 gccatgaacc gattgtttca cggtcattca cggaacgctc cgtcaagccc acggcgccga
     1741 aggcgccgcg ctcatcgggc acgagaagga gcgtcggcac tgcgatccgt cgttcgcgtc
     1801 aagccccgcg ccccgccaga gcgatggccg cgggcatcga cgcggcctcg atggcccgtc
     1861 gatgccgaag cccctcacaa aacccgcgaa tgttttcgtc caccggctga cacgccggtg
     1921 cggcagggtg ggctggggct tcgatcgcag gcgcgaccgg cgcgacgcgg gtggaagcct
     1981 ccggtccgcc aatcaccaca ttgaacgcgt cacgctcgcg agcgcgagga cgaagtcgaa
     2041 gccgaggaac ccgctgaatc cggccgtcca ggccggattc cgacgcacgg caagccggag
     2101 agcggagccc cagcccggcc tgtcgctcac gtagcgcagg cctgcgcgaa gcggcaacta
     2161 cacatcggcg gccaccatcc caaagtccac gacgccatag gacggcaggt cgaacgcctc
     2221 cgccctaccc cgccctgccg ctgatccact gcggcgtgca gcgcagcacc tcgtcgagcg
     2281 tcgtcaaccc ctcggccacc ttcatcaggc cggccaggcg cagcgggcgc atgccttcga
     2341 gcacgccctg gcgcgacagc ttggccacgt ccagcgtcgg gtggatcgct tccttgagcg
     2401 gctcggtgat gggcatcagc tcgtacagcg cggcgcggcc gcggtagccg gtgttgcggc
     2461 aggccgggca gcccaccgcc ttcatcggcc gcagccgccc gcccagcttc cagggctgcg
     2521 ccagctcctc gatcatcctg acggtgtcgg ccgactcgtc gggcaccttg cacgaggcgc
     2581 acagcgtgcg cgccaggcgc tgcgccagca cgccgatcac ggtggccgag atcaggtacg
     2641 gcggcacgcc caggtcgacc aggcgcgtga tggccgacgg cgcgtcgttg gtgtgcagcg
     2701 tcgagaacac gaggtggccg gtcagcgagg cctggatggc catctcggcg gtctccagat
     2761 cgcggatctc gcccaccatg atgatgtccg gatcctggcg catcagggag cgcaggccgg
     2821 cggcgaagtc caggtccagc gcgggctgca cctgcgtctg gttgaaggcc gcatcgatca
     2881 tttcgatcgg gtcctccacc gtgcagacgt tgacctcctc ggtggccagc ttcttgagcg
     2941 tcgagtacag cgtggtcgtc ttgcccgagc ccgtggggcc ggtgaccagg atgatgccgt
     3001 gcgcctgcgt cgtcagcgtc tgccagcgtt cggcctcggc cgggttgaag ccgagcgcgt
     3061 cgaaggcctt gtcgacggtg ccggggtcga agatccgcat cacgaccttc tcgccgaacg
     3121 ccgtgggcag cgtcgacagg cgcatttcca cctcggcgcc gggcatgccg cccgcgccat
     3181 cggggcgctt ggtcttgatg cggccgtcct gcgggcggcg gcgctcgatg acgtccatgc
     3241 gcgccagcag cttgatgcgc gcggtcatcg cgttcatcac cgcgggcggc acctgctgcg
     3301 cggtgtgcag cacgccgtcg atgcgcaggc ggatcgcgcc catgtcgcgg cgcggctcca
     3361 ggtggatgtc gctggcgcgc tgctcgaacg cgtactgcca caaccaatcg accacctgga
     3421 cgacgccctg gtcgttggcg tcgagctggt ccttgttctt gccgagctcg accagctgct
     3481 cgaagctgtt ggctgccgcc gcgcccgtcg tcttgttggc gaagcgcacc gacgcggcca
     3541 gggagtagaa ctcgggcgtg tagcgcgtga tgtcctcggg atgcgccagc tgcagccgca
     3601 ccgggcgctt cacgagcgcc tcgatctcgg gcacccaggc cacgtcgaac ggctcgcagg
     3661 tggcgatcac cacttccttg gcgttgaccg tgacgggcag cgcgcggcgc gactcggcgt
     3721 acgccaccga catcacctcc gccacgcgcc cgacgtccac ccgcaacggg tcgatgcggg
     3781 cgtacggcag ctcgaagcgc gcggccagcc agcgcgccag cgcgtcggtg tcgagcacgc
     3841 ggttgtcgcg gatgtcgacc aggctcgcgc cgcccaggcg ctgcagcggg tgcagcccgg
     3901 acgcgctggt gccgaagcgg cgcaggagct tgtcggcctc gtcgtgcgcg agcgcgccgt
     3961 cgtcgcgcag ccacttgagc agctggcgca gctcgaggcg cccacgtggc gccacgtggc
     4021 gggtggcggg cgcggccggg gacttggcgt ggggagtggc ggacatggcg tctttctcag
     4081 agggacagcg gccgcaccat gcgcagccac ttgcgcgccg gcaggccgaa gcgcgattcg
     4141 atgttatccg cccgcgcgac cagcgtgtcg cgatcgggca ccgtgacgcc gcggccggcg
     4201 acgaccaccg tgttgccctc gcgcgtgggc cgcaggctcc agacctggtc ggcaccgaag
     4261 gccgaggcga tgcgcgccgc gctcgccgcg aagctggcgt cgcggccgaa caggttgacg
     4321 gccatcagcc cgccgtggac cagcacgccg cggcaggccg cgtagaaggc gtcggagtcg
     4381 agcaccggcg cggcggcgtc gtggtcgtac aggtcgacgc tgagcacgtc ggccacctgc
     4441 aggttcgccg gatcgcgcac ccaggcgccc gcgtcggcct ccagcaccgc cagccgcgca
     4501 tcgtcggccg gcagctggaa ccacatgcgg caagcgctga tgacggtcgg gttgagctcg
     4561 acggccgtcg tgcgcatgcg catcaccttg tgcgtgaagc gtgtgatcga ggccgcgccc
     4621 aggcccagct gcacggcgtg gccgtggtcg acctcgtcct gcggccgcca cagcatccag
     4681 gccatcatgc gctggacgta ctcgagctcg atcgcctgcg gcttgcgcac gcgcatcgcg
     4741 ccctgcaccc acggcgtgcc caggtgcagg tagcgcacgc cgtcctgctc ggacatggtg
     4801 gcgggttcga gggtctggcg cgggcgggtc atggggcggc ggtggtcgag ggcgccagat
     4861 cgtagcgcga gaagacgtcg cgccaggccg ccagcttgcg ctcgaagctc caggacgcgt
     4921 tggcggggct ggtggacggc agcgtgtgca cgtccacgcc cagttgctcg gtgagccggc
     4981 gcgagcgggc cgattcggcg ccgttgtggg ccacggcacg caggccgggc gcgagcgcgg
     5041 gcaggcgcgc gaggtcgttg ggctcgggcg cgcggatcgc gctgtcgagg ctgccttccc
     5101 gctcgcagcg gcggtagacg tcccacaggc cgacgcgatg ccggcgcagc gcgtccagcc
     5161 gcgcggcgta gtccagggcg acgagatcct cgtcccacag cgtcgagagc agccgccaca
     5221 acacgttgcg cgggtggccg tagtactgct gcgcggccag cgaggcgacg ccggggaagc
     5281 tgccgaggat cacgaggcgc gtgtcgacgt cgatgacggg agcgaggcct tcgagcatcg
     5341 gcccgattgt gcccgcgcga cgttggcggc cggttgcggc ggcgtgtcgg gatcgaccag
     5401 gacgcgcgct ccgcccatcc gcagtagcat cgccagcgac gggacgacgt cccggacttt
     5461 cgaggagcac ccatggcccg cgcacggcgc acgatccttc cctggctggc cgccgcggcc
     5521 accgtcgtcg ccgcttccgc gccggcgcag gacgtgcaca tgcgtcacct ggccaagggc
     5581 atgaccaccg ggaagacgcc cgccaacgcg ttcatggtca tccgcaccgc cgacgacttc
     5641 gcccgcgcgc tgaaccagcg gatgaacgtc gagggccacc cggcggcgga cgcccgcggg
     5701 acgatcgact tcgacaccga gatggccgtc ggcgtcctgc tgtccaaccg gcccacggcc
     5761 tgcgccgggg tcgacatcac gtcggtctcg aaggacggca tcgtctcggt ggtgcactac
     5821 cgcgagcgca ggcgcaagcc gcacgagacc tgcgtggcga ccgtcacgtc gccgttcgac
     5881 ttcgtggcga tccccaagtc cggctcgccg atccgcttcc aggccgacgg cgaaccgtga
     5941 cccgcgccgc cttcacttgg tgacggcgtc cacgcccttg tggatggcgt ggccggtggc
     6001 cttcacccct tcgtggatgg catgccccgt cgccttgacg ccctggtgca cggcgtggcc
     6061 ggtggccttg acgccggtgt gcaccgcgtg gcccgtggcc ttgacgcctt cgtgcacggc
     6121 gtggccggcc ttgtgcacgt cctcgcgcac gccgctgtcg gcgacctgcg tgtcgtgcga
     6181 ctttgcgtag gcctcgtcca tgcgctgctg gcgggcggcg tcggcgccgt cctgcgcgaa
     6241 ggcgtggacg gacaggccga gggcggcggt gacgagaagg acgtggagct tgttcatggt
     6301 gtggttcctt gcgagagctg cctgctcgac agcggtgctg ctcgacggca agcgggatgc
     6361 ccgctacttg agcagcgtgg ccttgaaatg catccagacc agcatgccgc cgtcgacttc
     6421 gggcgccgcc gggccgacgg cgcgttcggc cagccgattg tccgccacga ggccgggaat
     6481 gacatgcccg ctgccgtgca ggacgacctg ctgcgacatc tggccgccga cgcaatggcc
     6541 gcgcacgatg cgctcggccc agcggtggcg gccgtcctcg gcctggtcgg tgatggcctc
     6601 gcgcgcgacc ggcgcggcgc agccggcgcg gtcggcccag aagcggaagg tgtcgtcggc
     6661 cgagcgcagg ccggcctgga tctcgccgcg gcgcaccgtg ccctccacct ggccgccgaa
     6721 cggcacggac ggatcctcgg tgccgttgac ggccagccag ggcagcggcc gcggcgactt
     6781 gcagttgcgc gccagcgcgt cgggcatcgt cgacagcagg ctggcggcgg cgcgcagcgt
     6841 ctcgccggcg tcgcaggcga agcgcatcgt catgaagccg ccgtgcgagg agccgaccat
     6901 gaagaccgcc gacgggtcgc cgtggtcctg cgcgatgacc tcggcgatga ccgccttgag
     6961 aaagcgcacg tcgtcgaccg tggcggtggg gctgcgctgg tcgttccagt ggcgg
//
LOCUS       DATCRO010000380         4854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_960618, whole genome shotgun sequence.
ACCESSION   DATCRO010000380 DATCRO010000000
VERSION     DATCRO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960618"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VIN75_10290"
     CDS             <1..876
                     /locus_tag="VIN75_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEY8974595.1"
                     /translation="VCLDSGAGNYDQLWLTTSLRGNITGTLKVEILTEGIHSGDASGL
                     VPSSFRILRHVLDRLEDSATGRLLPDSFHCQIPSDRIEQVKAAAAILGDEVWKRMPWA
                     CGADGGPTLPTTTDPTEALLNRTWRPTLSVTGVDGFPEIANAGNVLRPFSAFKLSLRL
                     PPLVEANEAAARLKALLEDNAPYNAKVTFQPDGRMGAYGATGWNAPVLDSWMETALNA
                     ASQAHWGEPLGYIGQGGTIPLMNLLQQGFPKAQMMVCGVLGPKSNAHGPNEFLHLPYA
                     KRLTAAVAQVIAAHP"
     gene            complement(1112..1552)
                     /locus_tag="VIN75_10295"
     CDS             complement(1112..1552)
                     /locus_tag="VIN75_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEY8974596.1"
                     /translation="MDARELEAYLHAHIPLSAAMQVSVRAIDADGVTLAAPLAPNINH
                     RETVFGGSASAVAILAAWSLLHTRMLAEGLHVRLVIQSNSMDYDKPIFGAFTARASLA
                     EPDQWPRFMRMLARMRRARMRVNSVLESDGAVVGRLTGEFVALA"
     gene            complement(1564..1773)
                     /locus_tag="VIN75_10300"
     CDS             complement(1564..1773)
                     /locus_tag="VIN75_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974597.1"
                     /translation="MRIIAILLIVLGALALIYGGFSYTQDKTAAKLGPIELNVKETKT
                     VPIPMWAGIAGIVVGGFLLVAGGRR"
     gene            complement(1904..2404)
                     /locus_tag="VIN75_10305"
     CDS             complement(1904..2404)
                     /locus_tag="VIN75_10305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974598.1"
                     /translation="MDIAPARAADAADLLVLRNHYIAHSFATFEEVPLTPAAVRDWMA
                     AFGDAGPHRLLVARDGPRLLGYCSSQAYRAHPAFARTIETSVYVAPGAAGRGVGSALY
                     ERLFAELAGQGLHRAVVGIALPNEASVRLHERSGFRAVGVFDEYAYKHGRAISSQWMQ
                     RAMAAP"
     gene            2585..4453
                     /locus_tag="VIN75_10310"
     CDS             2585..4453
                     /locus_tag="VIN75_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8974599.1"
                     /translation="MASSLNSPTPQERWHRFTAATVRAFHRYATWLVSLSWWRFGWYA
                     LVLIIVTGLLQKLPPFSLTYYETIVDSASHRKARMRTPPSPPSMPAIAMPEHPAQPEQ
                     PEHPEKVEPPEKPEKPQAPPSPTPAPSPKPDDSKSVDIKLPGIVVNGKDHTVKNKGVN
                     ITFTSDGIHISSDDGADEAAANAKAASAAASAVQAAKALEAATAAKAGATTARERAEA
                     AKLEAAARRDMALAEAMTQKAAAEAARAARLGGVAASAAAQASSAAPAATAAASQAAV
                     DMVVGGQHVRIDVPTQATAEEVRDAIEQARSAIVEQIQDDAEKRRAAEQEAADRATEQ
                     ADKARQHDDEDDDGDSSDNKHRTMHARTINTVPLSMLAVWWILGSMLIKITYKGRMQA
                     EVKAAEATETAEAESLRRQVLEARMAMMQAQVEPHFLFNTLASIDHLIEFDPKRASQM
                     QRNLISLLRASMPSMRSTDSGLRPLDLELAVVRPYLEILKVRMEERLTTEIDVPDGLL
                     SAEFPPMMIQTLVENSIKHGLEPKAEGGHLSVKAEIRHGKLAVTVCDTGLGFEAAAQG
                     STHGTGVGLANIRERLALLYGPKASLTIANNPVGGTIVTITVPYTTHTESDEGAKA"
     gene            4450..>4854
                     /locus_tag="VIN75_10315"
     CDS             4450..>4854
                     /locus_tag="VIN75_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974600.1"
                     /translation="MTDSIDPLDHAPEPDDRPAGMAASAPVATPAVPAVAAAPVPYRP
                     RPSGWARVFRWWLGLSVLAFLGVVLCVWIGVGHGDFSPLHIVVADDGADGITINGLSD
                     AGGALLAVGAGLLALLLLLLIPLLVLIVVAAVA"
BASE COUNT          771 a         1820 c         1650 g          613 t
ORIGIN      
        1 gtctgcctcg attcgggcgc cggcaactac gaccagctgt ggctcaccac cagcctgcgc
       61 ggcaacatca ccggcacgct gaaggtcgag atcctcaccg aaggcatcca ctccggcgac
      121 gccagcggcc tcgtgccgtc cagcttccgc atcctgcgcc acgtgctcga tcgcctggag
      181 gactcggcca ccggccgcct gctgcccgac agcttccact gccagatccc gagcgaccgc
      241 atcgagcagg tcaaggccgc cgccgccatc ctcggcgacg aggtgtggaa gcgcatgccg
      301 tgggcctgcg gcgccgacgg cggccccacg ctgcccacca ccaccgaccc gaccgaggcg
      361 ctgctgaacc gcacctggcg cccgacgctg tcggtcacgg gcgtggacgg cttcccggag
      421 atcgccaacg ccggcaacgt gctgcgcccg ttcagcgcat tcaagctgag cctgcgcctg
      481 ccgccgctgg tcgaggccaa cgaggcggcc gccaggctca aggcgctgct ggaggacaac
      541 gcgccataca acgcgaaggt caccttccag cccgacgggc gcatgggcgc atacggcgcc
      601 accggctgga acgcaccggt gctcgattcg tggatggaga cggcgctcaa cgccgccagc
      661 caggcgcact ggggcgaacc gctgggctac atcggccagg gtggcacgat cccgctgatg
      721 aacctgctgc agcaggggtt cccgaaggcc cagatgatgg tctgcggcgt gctgggcccc
      781 aagagcaatg cgcacggccc caacgagttc ctgcacctgc cctacgcgaa gcgcctgacg
      841 gccgccgtcg cacaggtcat cgcggcgcat ccctgagtcg cgtcggcgcg cgcgtgcagg
      901 agcgggtgcc gcgcgtcacg acgtgccatc ctgcgcgccg tcgcaggatc cccgcgccgg
      961 gatgcgctgc ctcgacggtg gatcctgcga ctccgcttcg cttcgcgcag gatgacggga
     1021 ccggcgcttc gcgtgaggca ccgctgccgc cgcacgccaa ccaagtgcca ccctgtcccc
     1081 agtcgcagga tgacggcgga agggaaggtg gctacgccag cgccacgaac tcgccggtca
     1141 gccgcccgac caccgccccg tcgctctcca gcaccgagtt cacgcgcatc cgcgcacgcc
     1201 gcatgcgcgc gagcatgcgc atgaatcgcg gccactggtc cggctcggcc agcgacgcgc
     1261 gcgccgtgaa ggcgccgaag atgggcttgt cgtagtccat cgagttgctc tggatcacca
     1321 gccgcacgtg caggccctcg gccagcatgc gcgtgtgcag cagcgaccag gccgccagga
     1381 tcgcgacggc cgaggcgctg ccgccgaaca ccgtctcgcg gtggttgatg ttgggcgcca
     1441 gcggcgcggc cagcgtcacg ccgtcggcat cgatcgcgcg caccgacacc tgcatcgccg
     1501 ccgacagcgg gatgtgggcg tgcaggtagg cctcgagttc gcgggcgtcc atgcggtgcc
     1561 cgctcaacgc cggccgcccg ccaccagcag gaagccgccg acgacgatgc ccgcgatgcc
     1621 ggcccacatc gggatcggca cggtcttggt ttccttgacg ttgagctcga tcgggccgag
     1681 cttggccgcg gtcttgtcct gggtgtagct gaagccaccg tagatcagcg ccagggcgcc
     1741 gaggacgatc aggaggatgg cgatgatgcg catgggtcgg tctcgttgcg gatgcggacg
     1801 ggccgcgatg acgcgagcgt agcctgcccg gccgtggccc cgtgtaggcg caggccgcgc
     1861 atcgcccgcc cgccagccgg ggttgacccg atgctgcggc gcgtcatggc gccgccatcg
     1921 cccgctgcat ccactgcgag ctgatcgcgc ggccgtgctt gtacgcatac tcgtcgaaca
     1981 cgcccaccgc ccggaagccg gaacgttcgt gcagacgcac ggacgcctcg ttgggcagcg
     2041 cgatgcccac cacggcgcga tgcagcccct ggccggcgag ctcggcgaac agccgctcgt
     2101 acagcgccga gccgacgccc cggcccgcgg caccgggcgc cacgtagacg ctggtctcga
     2161 tcgtgcgcgc gaaggccggg tgggcccgat acgcctggct gctgcagtac ccgagcaggc
     2221 gcgggccgtc gcgtgcgacg agcagtcggt gcgggcccgc gtccccgaag gcagccatcc
     2281 agtcccgcac ggcagccggc gtcagcggca cctcctcgaa ggtcgcgaac gagtgggcga
     2341 tgtagtggtt gcgcaggacc agcaggtcgg cggcgtcggc ggcgcgggcg ggggcgatgt
     2401 ccatggtccg ggttctcctg ggcggccatt ctcggggcag gtggccggcg cggctgcccg
     2461 gtccgtccgc cgcccctgca gcctgtcacc ccaaacgtga cgccaatcgc ttgcgccctg
     2521 acagcggccg agcggccacg tagagtgcaa ccatcgacaa agagatccgt tgggctccca
     2581 gaacatggct tcctccctca actcgccgac gccccaggaa cgctggcacc gcttcaccgc
     2641 cgccaccgtc cgggcgttcc atcgctacgc gacctggctg gtctcgctga gctggtggcg
     2701 gttcgggtgg tacgcgctgg tgctgatcat cgtcaccggc ctgctgcaga agctgccccc
     2761 gttcagcctg acgtactacg agacgatcgt cgactcggcc agccaccgca aggccaggat
     2821 gcgcaccccg ccgtcgccgc cgtccatgcc cgcgatcgcg atgcccgagc atccggccca
     2881 gccggaacag ccggagcacc ccgagaaggt ggaaccgccc gaaaagcccg agaagccgca
     2941 agctccgccg agccccacgc ccgccccgtc gcccaagccg gacgattcca agagcgtgga
     3001 catcaagctg cccggcatcg tcgtcaacgg caaggaccac accgtcaaga acaagggcgt
     3061 gaacatcacg ttcaccagcg acggcatcca catcagcagc gacgacggcg ccgacgaggc
     3121 cgccgccaac gcgaaggccg cgtccgccgc cgccagcgcc gtgcaggccg ccaaggccct
     3181 ggaagcggcc accgccgcca aggcgggcgc caccacggcc agggagcgcg ccgaggccgc
     3241 caagctcgag gcggccgccc gccgcgacat ggccctggcc gaagcgatga cgcagaaggc
     3301 cgcggccgag gccgcccggg ccgccaggct cggcggcgtc gcggcgagcg ccgcggccca
     3361 ggcgagttcg gccgcgccgg cggcgaccgc cgcggcgtcg caggccgcgg tcgacatggt
     3421 cgtcggcggc cagcacgtgc gcatcgacgt gccgacccag gccaccgccg aggaagtgcg
     3481 cgacgccatc gagcaggcgc gctcggcgat cgtcgagcag atccaggacg acgcggagaa
     3541 gcgccgggcc gccgaacagg aagccgccga ccgcgcgacc gagcaggccg acaaggcgcg
     3601 ccagcacgac gacgaggacg acgacggcga ctcgtccgac aacaagcacc gcacgatgca
     3661 tgcgcgcacg atcaacacgg tgccgctgtc gatgctggcc gtgtggtgga tcctggggtc
     3721 gatgctgatc aagatcacgt acaagggccg catgcaggcc gaggtgaagg ccgccgaggc
     3781 caccgagacg gccgaggccg agtcgctgcg ccgccaggtg ctggaagcgc gcatggccat
     3841 gatgcaggcg caggtcgagc cgcacttcct gttcaacacg ctggcctcca tcgaccacct
     3901 gatcgagttc gaccccaagc gcgccagcca gatgcagcgc aacctgatct cgctgctgcg
     3961 cgcgagcatg ccgtcgatgc gctccaccga ctccggcctg cgtccgctgg atctcgagct
     4021 ggccgtcgtg cggccctacc tcgagatcct gaaggtgcgc atggaagagc gcctgaccac
     4081 ggagatcgac gtgcccgacg gcctgctgtc ggccgagttc ccgccgatga tgatccagac
     4141 gctcgtggag aactcgatca agcacggcct ggagcccaag gccgagggcg gccacctgtc
     4201 ggtcaaggcc gagatccgcc acggcaagct ggcagtcacc gtctgcgaca ccggcctcgg
     4261 cttcgaggcc gccgcccagg gctcgacgca cggcaccggc gtcggcctcg ccaacatccg
     4321 cgaacgcctg gcactgctgt atggcccgaa ggcctcgctg acgatcgcca acaacccggt
     4381 cggcggcacg atcgtgacca tcaccgtgcc ctacaccacc cacaccgaaa gcgacgaagg
     4441 agccaaggca tgaccgattc catcgacccc ctcgaccacg cgcccgaacc cgacgaccgc
     4501 cccgccggca tggccgcgtc cgctcccgtc gccacgcccg ccgtcccggc cgtggccgcc
     4561 gcgccggtgc cctaccggcc gcgccccagc ggctgggcgc gcgtcttccg ctggtggctg
     4621 ggactgagcg tgctggcctt cctcggcgtc gtgctgtgcg tgtggatcgg cgtcggccac
     4681 ggcgacttct cgccgctgca catcgtcgtc gccgacgacg gcgccgacgg catcacgatc
     4741 aacggcctga gcgacgcggg cggcgcactg ctggcggtcg gcgccggcct gctcgcactg
     4801 ctgctcctgc tgctgatccc gctgctggtc ctgatcgtcg tggcggcggt ggcg
//
LOCUS       DATCRO010000381         6258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_555591, whole genome shotgun sequence.
ACCESSION   DATCRO010000381 DATCRO010000000
VERSION     DATCRO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555591"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VIN75_10320"
     CDS             <1..573
                     /locus_tag="VIN75_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY8974601.1"
                     /translation="EETQIELVNIIESVGVTCVMVTHDQEEAMTMASRIAVMSEGRFL
                     QVGTPSEIYETPATRFVADFIGNVNLMDGTVAVDEADHVIIDCADCKHYVGHGITGTV
                     GMPVTVALRPEKITLQREKPTDPYNCVGGKIEELSYFGSSTVFRVKLASGKVLAVSMA
                     NTERHSDSALTWGDVVWATWSPQAHVVLTQ"
     gene            587..1537
                     /locus_tag="VIN75_10325"
     CDS             587..1537
                     /locus_tag="VIN75_10325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974602.1"
                     /translation="MEASDNKKAGWRPSWWRGRTAVIGVPYFWLLLFFLAPFLIVANY
                     SVSEMGAIRAEDISSVSDGALRLVMKYTNYQRLWPTQDTLYLYAYWASIKYAFVTTCF
                     CLLIGYPFAYFMARAKASLQPTLLMLVMLPFWTSFLLRVYAWKGLLDDGGWVANIIAW
                     THLDRLLVMMGVIEVPGQLMHTTFSLIIGMTYTYLPFMILPLYANLSKMDLRLLEAAS
                     DLGATPWVAFWRVTVPLSKAGIIAGSMLVFIPCVGEYVTPDLLGGPSTLMIGRVIWDE
                     NFNNNDPPMAAAVAVVLVLLIVVPLAYFNKYQAEAQEARK"
     gene            1647..2507
                     /locus_tag="VIN75_10330"
     CDS             1647..2507
                     /locus_tag="VIN75_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8974603.1"
                     /translation="MRNRALFGTKFAKGFGRAWLVGGFLFLYLPMVALMVFSFNHSRL
                     ASHWGGFSLEWYRKLADDTEIINGLWLSLKIAVTSATGSVILGTMAAFTLVKYKRFRG
                     RTFFSGMVNAPLVMPEVIQGLSLLLMMVAIQRLIGFPERGFFTIWLGHLMIGMSYAAV
                     VVQARLQDLNPQLEEAAMDLGARPWQVFRLVTFPMITQSLISAWLLTFSLSLDDVVSS
                     AFLSGPGATTMPLVIFSRARLGLDPSVNAVATVMIVVVAICVAAASIWVARAERRRAA
                     EISAAQRDDS"
     gene            2553..3965
                     /locus_tag="VIN75_10335"
     CDS             2553..3965
                     /locus_tag="VIN75_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3138 family protein"
                     /protein_id="HEY8974604.1"
                     /translation="MKSLRPTALACAITLAFGAATPASAQTNAEVLKELAALKARIAE
                     LEKKLAAVPEAPADGQQAQWGMTPEQAADFNKLVVKTEALEDANESHGYKDLKISGYM
                     DPTFIWNKDQNRAGFQFLSPVSEDGYNYDDSYFGTVQLDFQKELEGGTKWRLTLMPQR
                     GAGTVADPGGMSIVHEASFSIPLGDLQTRLIGGQIPDWSGYEYIQPTTTKLVTRGMLL
                     DFTEPTTYTGIGGEIIRGKWDVKALVANFNTAMNQPKQMSPVLAYRVDYSKGEFSGWG
                     LAGVEGKVHNFNYDTGVIGNASQNTMLHLFEADAYFIRGDLTLQGQVGFGKQKGAAIT
                     PDADGNARDAQWWGLSGLAAYKFEPRLEGVVRADYINDARNGGGLLGYTGYYDGQGAL
                     GDTRNGFGVSPNCTLGADCKGANRAALAMGFNYLLTANTTIKAEYRLDMANQAVFLDV
                     KTGTFRKNNSLLGTTMVVSF"
     gene            4057..5556
                     /locus_tag="VIN75_10340"
     CDS             4057..5556
                     /locus_tag="VIN75_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8974605.1"
                     /translation="MSTTLNWSERATALKIDGRMLVDGKRVATQTGETFAKHSPIDAR
                     LLGPIARGVAADVDAAVRSARAAFEDGRWAGKAPAQRKKVLQRFAELILGAREELALL
                     ETLDMGKPIQYSLAVDVPAAARCIAWYAEAVDKIYDEIAPTASSALALVTREPMGVIG
                     VIVPWNYPMIMAAWKLGPALAAGNSVVLKPSEKSPLTALRMAELALEAGLPPGVFNVV
                     PGYGHEAGEALALHMEVDAIGFTGSTRVGRRMYEYAGKSNLKRVYNELGGKSAFVVFD
                     DFADVARAARTVVGGMFFNQGESCNAPSRVLVHERIADEFVATLRTEAPKYAPADPLD
                     ARTEMGALVDDTQLRTVLGYIESGSAQGARIVSGGRQARAETGGYYVEPTVFDNVTND
                     MKIAQEEIFGPVMSVLRFKTEDEAIALANNSSYGLQASCWSDNINRAHRVARALRAGT
                     VHVNSYDEDDITVPFGGYKQSGNGRDKSLHAFDKYTELKTTWIRIDGKD"
     gene            5689..>6258
                     /locus_tag="VIN75_10345"
     CDS             5689..>6258
                     /locus_tag="VIN75_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEY8974606.1"
                     /translation="MTTTPSHDELATLQSTLRQQGVHTLLAQFVDLHGTPKGKWVPLH
                     QLPTLVRDGAGFAGPSIWGTALPRTGPRSEYYARADLRTLTVLPWMPGVARVACDGFV
                     AGAPFDHCPRQVLRRATARLAQRGWTLQVGIEPEFFLLKRQDGRWLPFDDADRLDKPS
                     YDLKSLPRQAAFLHELRGALEAAGMKVQQT"
BASE COUNT         1023 a         2188 c         2083 g          964 t
ORIGIN      
        1 gaggagacgc agatcgagct ggtcaacatc atcgagtcgg tgggcgtgac ctgcgtgatg
       61 gtcacccacg accaggagga ggcgatgacg atggcctcgc gcatcgcggt gatgagcgag
      121 ggccgcttcc tgcaggtggg cacgccgtcg gagatctacg agacgccggc cacccgcttc
      181 gtggccgact tcatcggcaa cgtcaacctg atggacggca cggtggccgt cgacgaggcc
      241 gaccacgtca tcatcgactg cgccgactgc aagcactacg tcggccacgg catcaccggc
      301 accgtgggca tgccggtcac cgtggcactg cgccccgaga agatcacgct gcagcgcgag
      361 aagccgaccg acccgtacaa ctgcgtgggc ggcaagatcg aggaactgtc gtacttcggc
      421 tcgtccacgg tgttccgcgt gaagctggcc agcggcaagg tgctggccgt gagcatggcc
      481 aacaccgagc gccacagcga cagcgcgctc acctggggcg acgtcgtgtg ggcgacctgg
      541 tcgccgcagg cccacgtcgt gctgacgcag taagggggcg cacgacatgg aagcttccga
      601 caacaagaag gccggctggc gcccctcgtg gtggcgcggc cgcacggcgg tcatcggcgt
      661 gccgtatttc tggctgctgc tgttcttcct cgcgccgttc ctgatcgtcg ccaactactc
      721 ggtctccgag atgggcgcga tccgcgcgga ggacatctcc agcgtctccg acggcgcgct
      781 caggctggtg atgaagtaca ccaactacca gcgcctgtgg ccgacccagg acacgctgta
      841 cctgtacgcg tactgggcca gcatcaagta cgcgttcgtc acgacgtgtt tctgcctgct
      901 gatcggctac ccgttcgcct acttcatggc gcgcgccaag gcgtcgctgc agcccacgct
      961 gctgatgctg gtgatgctgc cgttctggac gtcgttcctg ctgcgcgtgt atgcctggaa
     1021 gggcctgctg gacgacggcg gctgggtggc caacatcatc gcgtggacgc acttggacag
     1081 gctgctcgtg atgatgggcg tgatcgaggt gcccgggcaa ctgatgcaca ccacgttctc
     1141 gctgatcatc ggcatgacgt acacgtacct gccgttcatg atcctgccgc tgtacgcgaa
     1201 cctgtcgaag atggacctgc gcctgctcga ggccgcgtcc gacctcggcg ccacgccgtg
     1261 ggtcgcgttc tggcgcgtga cggtgccgct gtccaaggcc ggcatcatcg ccggctcgat
     1321 gctcgtgttc atcccctgcg tcggcgagta cgtcacgccc gacctgctag gcggcccgtc
     1381 cacgctgatg atcggccgcg tcatctggga cgagaacttc aacaacaacg atccgccgat
     1441 ggccgccgcc gtcgccgtgg tgctcgtgct gctgatcgtg gttccgctgg cctatttcaa
     1501 caagtaccag gccgaggcgc aggaggcgcg caaatgaaca atcggacgcc agtcatttca
     1561 gaagcggacg ccgcgccggg ccgccccaag caaggccgcg ccccctcggg gggtcggacc
     1621 gcgtactcgc gggacggggg gcagtcatga ggaaccgcgc tttgttcggc acgaagttcg
     1681 ccaagggctt cggccgcgcc tggctggtcg ggggcttcct gttcctgtac ctgccgatgg
     1741 tcgcgttgat ggtgttctcg ttcaaccatt cgcggctggc gtcgcactgg ggcggcttct
     1801 cgctcgagtg gtaccgcaag ctggcggacg acaccgagat catcaacggc ctgtggctgt
     1861 cgctcaagat cgccgtgacc tcggccaccg gctcggtgat cctgggcacg atggcggcgt
     1921 tcacgctggt caagtacaag cgcttccgcg gccgcacctt cttcagcggc atggtcaacg
     1981 ccccgctggt gatgcccgag gtgatccagg gcctgtcgct gctgctgatg atggtggcga
     2041 tccagcgcct catcggcttc ccggagcgcg gcttcttcac gatctggctg ggccacctga
     2101 tgatcggcat gtcgtacgcg gcggtcgtgg tgcaggcgcg cctgcaggac ctgaacccgc
     2161 agctcgagga ggccgcgatg gacctgggcg cgcggccgtg gcaggtcttc cggctcgtca
     2221 cgtttccgat gatcacgcag tcgctgatct cggcctggct gctcacgttc agcctgtcgc
     2281 tcgacgacgt cgtgtcctcg gcgttcctga gcgggcccgg cgcgaccacg atgccgctgg
     2341 tgatcttctc gcgcgcccgc ctggggctgg atcccagcgt caacgccgtg gccaccgtga
     2401 tgatcgtcgt cgtggccatc tgcgtggccg ccgccagcat ctgggttgcc cgcgccgagc
     2461 gccgccgcgc cgccgagatc agcgccgccc agcgtgacga ttcctgaatc gattcctgca
     2521 gacgttccaa aaacacactt gaggagtagt cgatgaaatc cttgcgtccc accgccctcg
     2581 cctgcgcgat caccctcgcg ttcggcgccg ccacgcccgc ctcggcccag accaacgcgg
     2641 aggtgctcaa ggagctcgcc gcgctgaagg cccgcatcgc cgaactcgag aagaagctgg
     2701 ccgccgtgcc cgaggcgccc gccgacggcc agcaggccca gtggggcatg acgcccgaac
     2761 aggccgccga cttcaacaag ctggtcgtca agaccgaggc gctggaggac gcgaacgagt
     2821 cgcatggcta caaggacctg aagatcagcg gctacatgga cccgacgttc atctggaaca
     2881 aggaccagaa ccgcgccggc ttccagttcc tgagcccggt ctccgaggac ggctacaact
     2941 acgacgactc ctacttcggt accgtgcagc tcgacttcca gaaggagctc gagggcggca
     3001 ccaagtggcg cctgacgctg atgccgcagc gcggcgccgg caccgtggcc gacccgggcg
     3061 gcatgtccat cgtgcacgag gcctcgttct cgatcccgct cggggacctg cagacgcgcc
     3121 tgatcggcgg ccagatcccc gactggtcgg gctacgagta catccagccc accacgacca
     3181 agctggtcac gcgcggcatg ctgctggact tcaccgagcc gaccacctac acgggcatcg
     3241 gcggcgagat catccgcggc aagtgggacg tcaaggcgct cgtcgcgaac ttcaacacgg
     3301 cgatgaacca gcccaagcag atgtccccgg tgctggccta ccgcgtcgac tactccaagg
     3361 gcgagttctc gggctggggc ctggccggcg tcgagggcaa ggtgcacaac ttcaactacg
     3421 acaccggcgt gatcggcaac gcgtcgcaga acacgatgct gcacctgttc gaggccgacg
     3481 cgtacttcat ccgcggcgac ctgacgctgc aaggccaggt cggcttcggc aagcagaagg
     3541 gtgcggccat cacgcccgac gccgacggca atgcccgcga cgcgcagtgg tggggcctgt
     3601 cgggcctggc cgcgtacaag ttcgagccgc gcttggaagg cgtcgtccgt gccgactaca
     3661 tcaacgatgc caggaacggc ggcggcctgc tcggctacac cggctactac gacggccagg
     3721 gcgcgctcgg cgacacccgc aacggtttcg gcgtctcgcc caactgcacg ctgggcgcgg
     3781 actgcaaggg cgccaaccgg gcggccctcg cgatgggctt caactacctg ctgaccgcca
     3841 acacgaccat caaggccgag taccgcctcg acatggcgaa ccaggccgtg ttcctggacg
     3901 tcaagaccgg caccttccgc aagaacaact ccctgctggg aaccacgatg gtcgtgagct
     3961 tctagactgg ctgagccccc cgggtcgacc accctacccg acccaccacg gggccggcca
     4021 cgagccggcc ccgtcgattt cgacaggaga cttctcatga gcaccaccct caactggtcc
     4081 gagcgcgcca ccgcgctcaa gatcgacggc cgcatgctgg tcgacggcaa gcgcgtcgcc
     4141 acgcagaccg gcgagacctt cgccaagcac tcccccatcg acgcacgcct gctgggtccg
     4201 atcgcgcgtg gcgtggcggc ggacgtcgac gcggccgtgc gctcggcgcg cgccgcgttc
     4261 gaggacggcc gctgggccgg caaggcgccc gcgcagcgca agaaggtgct gcagcgcttc
     4321 gccgagctca tcctcggcgc cagggaggaa ctggcgctgc tcgagacgct ggacatgggc
     4381 aagcccatcc agtactcgct ggccgtggac gtgcccgcgg ccgcgcgctg catcgcgtgg
     4441 tacgccgagg cggtcgacaa gatctacgac gagatcgcgc ccaccgcgtc gagcgcgctc
     4501 gcgctcgtca cgcgcgagcc catgggcgtg atcggcgtga tcgtgccctg gaactacccg
     4561 atgatcatgg ccgcctggaa gctgggtccg gcgctggccg ccggcaactc ggtcgtcctc
     4621 aagcccagcg agaagagccc gctgaccgcg ctgcgcatgg ccgagctcgc gctcgaggcc
     4681 ggcctgccgc cgggcgtgtt caacgtggtg ccgggctacg gccacgaggc cggcgaggcg
     4741 ctggcgctgc acatggaggt cgacgcgatc ggcttcaccg gctccacgcg cgtgggccgc
     4801 cgcatgtacg agtacgcggg caagtccaac ctgaagcgcg tctacaacga gctgggcggc
     4861 aagtcggcgt tcgtcgtgtt cgacgacttc gccgacgtcg cgcgtgccgc caggacggtg
     4921 gtcggcggca tgttcttcaa ccagggcgag tcctgcaacg cgccgtcgcg cgtgctcgtg
     4981 cacgagcgta tcgccgacga gttcgtcgca acgctgcgca ccgaggcgcc caagtacgcg
     5041 ccggccgatc cgctggacgc caggacggag atgggcgcac tggtcgacga cacgcagttg
     5101 cgcaccgtgc tcggctacat cgagtcgggc agcgcgcagg gcgcgcgcat cgtcagcggc
     5161 ggcaggcagg cgcgcgcgga gaccggcggc tactacgtcg aacccaccgt gttcgacaac
     5221 gtcaccaacg acatgaagat cgcgcaggaa gagatcttcg ggccggtgat gtcggtgctg
     5281 cgcttcaaga ccgaggacga ggcgatcgcg ctggccaaca actccagcta cggcctgcag
     5341 gccagttgct ggagcgacaa catcaaccgc gcccaccgcg tcgcccgcgc gctgcgcgcg
     5401 ggcaccgtgc acgtcaactc gtacgacgag gacgacatca ccgtgccgtt cggcggctac
     5461 aagcagagcg gcaacgggcg cgacaagtcg ctgcacgcgt tcgacaagta caccgagctg
     5521 aagacgacct ggatccggat cgacggcaag gactgacggc tcccagacgc tccggccggt
     5581 cgctgccccg ccgaggggga gcgcggatcg gctcgggtgc gtccctcgct ttcctcttcc
     5641 tggctggcct gcggccggct ggttcctgcg cgcaaaacga gtagctccat gacgacgacc
     5701 ccctcccacg acgagctggc cacgctgcaa tccaccttgc gccagcaagg cgtccacacc
     5761 ctgctcgcgc agttcgtcga cctgcacggc acgcccaagg gcaagtgggt gccgctgcac
     5821 cagctgccca cgctcgtgcg tgacggcgcc ggcttcgcgg gcccgtcgat ctggggcacc
     5881 gccctgcccc gcaccggccc gcgcagcgag tactacgcgc gcgccgacct gcgcacgctg
     5941 accgtgctgc cgtggatgcc cggcgtcgcg cgcgtcgcct gcgacggctt cgtcgccggc
     6001 gcgccgttcg accactgccc gcgccaggtg ctgcgccgag ccaccgcgcg cctggcgcag
     6061 cgcggctgga cgctgcaggt ggggatcgag ccggaattct tcctgctcaa gaggcaggac
     6121 ggccgctggc tgccgttcga cgacgccgac cgcctcgaca agccgtccta cgacctcaag
     6181 agcctgccgc gccaggccgc gttcctgcac gagctgcgcg gcgcgctgga ggccgccggc
     6241 atgaaggtgc agcagacc
//
LOCUS       DATCRO010000382         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_37813, whole genome shotgun sequence.
ACCESSION   DATCRO010000382 DATCRO010000000
VERSION     DATCRO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37813"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VIN75_10350"
     CDS             <1..222
                     /locus_tag="VIN75_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY8974607.1"
                     /translation="ALPAQAQDAEVIQLREQLKALQQALNARGLNAGDVDGQMGPATR
                     QALRAWQRSRDLPADGFPTLEQLAQLQTP"
     gene            219..692
                     /locus_tag="VIN75_10355"
     CDS             219..692
                     /locus_tag="VIN75_10355"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974608.1"
                     /translation="MTPPTIVPARVEDAPAMLALQKVAFEAEARACGSWEIPPLQETV
                     AGIETHIREATVLKAMDGDRLVGAIRGVTTGDTCLIRVLVVAQDQQGQGLGARLLAAI
                     EAAHPHVARFELTTNMVMVGNVRFYLRHGYEVVEQIRHAPTIRLAFMRKAVRPAQ"
     gene            complement(686..1066)
                     /locus_tag="VIN75_10360"
     CDS             complement(686..1066)
                     /locus_tag="VIN75_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974609.1"
                     /translation="MTPLLAALLAAALNLGSAADPDATESSLNYSIQFDESTGSAALS
                     CDGSSTGSCTFWFGDGHVDSARAAGSGTLAVGSAPAIVRVSAPHAAYCVGIDADTPPK
                     WPECTHGPLGGALDRTETVDYRRH"
     gene            1296..3380
                     /locus_tag="VIN75_10365"
     CDS             1296..3380
                     /locus_tag="VIN75_10365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8974610.1"
                     /translation="MTAVAPPPPVADPFESLNDEQRAAVCHGADGDPRALLVIAGAGS
                     GKTMTLAARVAHLIQRGADPTRLLLLTFSRRAAGEMTQRAGRIVHQALGLPATTRPPA
                     LPWAGTFHAIGARLLRDYADAIGLPVDFTVLDRADAEDLMAIVRQAQGLAATKLRFPQ
                     KGTCVAICSRVVNSQGTVDAVLRDAFPWCLGWETELRQLFAAYAQAKAQQHSLDFDDL
                     LLYWQALMEAPELASHVAGRFDHVLVDEYQDTNRLQAAILRGLQPDGRGLTVVGDDAQ
                     SIYAFRAAEVRNILDFPQQFPQPARVIALERNYRSTQPILEASNAVIAEAPERFAKTL
                     WSDRPSSARPRIVTVPDEAAQARQVADRVLAWREEGIRLRQQAVLFRTAHHSAAVELE
                     LTRRRIPFVKFGGLKFLESTHVKDVLAILRWAENPRSRLAGFRVAQLVPGVGPANARK
                     LLDAMDASGAPADALASHAMPAAASEDWAGFVALYERLRACPGWPADLEAVNAWYVPL
                     LQRLHDDAPARQADLAQLTQIAGGYANRERFLTELTLDPPAVSSDEAGPPHLDEDYMI
                     LSTIHSAKGQEWGAVQILNVVDGCIPSEMSTGDARQIAEERRLLYVGMTRARQHLELL
                     VPQRFFVTEQARLGDRHVYASLSRFITDKAGACFERVAAQRAAGSEGAAGPTGVPDVK
                     IDLAARVRDRWA"
BASE COUNT          470 a         1230 c         1247 g          446 t
ORIGIN      
        1 gcgctgcctg cgcaggcgca ggacgcggag gtgatccagc tgcgcgagca gctcaaggcg
       61 ctgcagcagg cgctcaacgc ccgtggcctg aacgccggcg acgtcgacgg ccagatgggg
      121 ccggccacgc gccaggccct gcgcgcctgg cagcgcagcc gcgacctgcc ggccgacggc
      181 ttcccgacgc tggaacagct cgcgcaactg cagaccccat gacgcccccg acgatcgtcc
      241 ccgcccgcgt cgaggacgcg ccggcgatgc tggcgctgca gaaggtcgcc ttcgaggccg
      301 aggcgcgcgc gtgcggatcg tgggagatcc cgccgctgca ggagacggtg gccggcatcg
      361 agacgcacat ccgcgaggcg accgtactca aggcgatgga cggcgaccgg ctggtcggcg
      421 cgatccgcgg cgtcaccacc ggcgacacct gcctgatccg cgtgctggtc gtcgcgcagg
      481 accagcaggg acagggcctg ggcgcgcggc tgctggcggc gatcgaggcc gcgcatccgc
      541 atgtcgcgcg cttcgagctg accaccaaca tggtcatggt gggcaacgtg cgcttctacc
      601 tgcgccacgg ctacgaggtc gtcgagcaga tccggcacgc gccgacgatc cgcctcgcgt
      661 tcatgcgcaa ggcggtgcgg ccggctcagt gacgccggta gtcgaccgtc tccgtgcgat
      721 cgagcgcgcc gcccagcggg ccgtgcgtgc actcgggcca cttgggcggc gtgtccgcat
      781 cgatgccgac gcaataggcc gcatgcggcg ccgagacgcg cacgatcgcc ggcgcgctgc
      841 cgacggccag cgtcccggag ccggcggcgc gggcgctgtc gacgtgcccg tcgccgaacc
      901 agaacgtgca gctgcccgtc gaggagccgt cgcaggacag cgcggcgctg ccggtcgact
      961 cgtcgaactg gatgctgtag ttcagcgagg attccgtcgc gtccgggtcg gcggcggagc
     1021 cgaggttcag ggcggcggcg agcagggcgg cgaggagcgg cgtcatgaac aacctcttcg
     1081 tgatgcgctg gagcagtggg cgccagatta gccatcgcgg cgcgccgcgc cggcccccgt
     1141 ttggggggcg tcccccatcg gtgggaatgc gtgcgtgatg cattggcgcg ccgctcgcgc
     1201 agggactggc catccccgtt tcggatggcg ccccgcagac cgcgctggac ggctgtcctg
     1261 accggcaggg tctgtatggg cgtacagttc ggcccatgac cgccgtcgcc cctccgccgc
     1321 ccgtcgccga tccgttcgag agcctgaacg acgagcaacg cgcggccgtg tgccatggcg
     1381 ccgacggcga cccccgcgcg ctgctggtca tcgccggcgc cggcagcggc aagacgatga
     1441 cgctggccgc gcgcgtggcg cacctgatcc agcgcggcgc cgaccccacg cgcctgctgc
     1501 tgctgacgtt ctcgcgccgc gccgccggcg agatgaccca gcgtgccggc cgcatcgtcc
     1561 accaggcgct cggcctgccg gccaccacgc gcccgcccgc gctgccctgg gccggcacct
     1621 tccacgcgat cggcgcgcgc ctgctgcgcg actatgccga cgcgatcggc ctgcccgtcg
     1681 atttcacggt gctcgatcgc gcggacgccg aagacctgat ggccatcgtg cgccaggcgc
     1741 agggcctggc cgcgaccaag ctgcgcttcc cgcagaaggg cacctgcgtg gcgatctgct
     1801 cgcgcgtggt caacagccag ggcaccgtcg acgccgtgct gcgcgacgcg ttcccgtggt
     1861 gcctgggctg ggagaccgag ctgcggcagc tgttcgcggc gtacgcgcag gccaaggcgc
     1921 agcagcacag cctggacttc gacgacctgc tgctgtactg gcaggcgctg atggaggcgc
     1981 ccgagctcgc gagccacgtc gccggccgct tcgaccacgt gctggtcgac gagtaccagg
     2041 acaccaaccg gctgcaggcc gccatcctgc gcggcctgca gcccgacggc cgcggcctga
     2101 cggtggtggg cgacgacgcg cagtcgatct acgccttccg cgccgccgag gttcgcaaca
     2161 tcctggactt cccgcagcag ttcccgcagc ccgcgcgcgt gatcgcgctg gagcgcaact
     2221 accggtcgac gcaacccatc ctcgaggcct ccaacgccgt catcgccgag gcgcccgagc
     2281 gcttcgccaa gaccttgtgg agcgaccggc cgtcgtcggc gcggccgcgc atcgtcacgg
     2341 tgcccgacga ggccgcgcag gcgcgccagg tggccgaccg cgtgctggcc tggcgcgagg
     2401 agggcatccg gctcaggcaa caggcggtgc tgttccgcac ggcgcatcac tcggcggcgg
     2461 tcgaactcga gctgacgcgg cggcgcatcc cgttcgtgaa gttcggcggc ctgaagttcc
     2521 tggagtccac gcacgtgaag gacgtgctgg ccatcctgcg ctgggccgag aacccgcgct
     2581 cgcgcctggc cggctttcgc gtggcgcagc tggtgcccgg cgtcgggccc gcgaacgcgc
     2641 gcaagctgct cgatgcgatg gacgcgtccg gcgcgcccgc ggacgcgctc gcgtcgcacg
     2701 cgatgcccgc cgccgcgagc gaggactggg cgggcttcgt cgcgctgtac gagcgcctgc
     2761 gcgcgtgccc cggctggccg gccgacctgg aggccgtcaa cgcctggtac gtcccgctgc
     2821 tgcagcgcct gcacgatgac gcaccggcgc gccaggccga tctcgcgcag ctgacgcaga
     2881 tcgccggcgg ctatgccaac cgcgaacgct tcctcaccga actgacgctc gacccgcccg
     2941 ccgtctccag cgacgaggcc ggcccgccgc acctggacga ggactacatg atcctgtcga
     3001 ccatccactc ggccaagggc caggagtggg gcgcggtgca gatcctcaac gtcgtcgatg
     3061 gctgcatccc gtcggagatg agcacgggcg acgccaggca gatcgccgag gagcgccggc
     3121 tcctgtacgt gggcatgacg agggccaggc agcacctgga gctgctggtg ccccagcgct
     3181 tcttcgtgac ggagcaggcc aggctgggcg accggcacgt gtacgcgtcg ctgtcgcggt
     3241 tcatcacgga caaggcgggg gcgtgtttcg agcgcgtggc ggcgcagcgg gcggcaggga
     3301 gcgaaggcgc ggcggggccg acgggcgtgc cggatgtgaa gatcgacctg gcggccaggg
     3361 tgcgagaccg ctgggcgtag ggcgggttag ccg
//
LOCUS       DATCRO010000383         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1016300, whole genome shotgun sequence.
ACCESSION   DATCRO010000383 DATCRO010000000
VERSION     DATCRO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016300"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VIN75_10370"
     CDS             complement(<1..345)
                     /locus_tag="VIN75_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY8974611.1"
                     /translation="MNNDLDTAHLVALAAALGWASGVRLYLVIFLTGLAGAMGWVTLP
                     PGLHILQSPVVLGASAILATIEFVADKVPAVNAFWDGLHTLMRVPGGAVLAAAVFGGD
                     QAQWTLVAALAGG"
     gene            complement(362..1147)
                     /locus_tag="VIN75_10375"
     CDS             complement(362..1147)
                     /locus_tag="VIN75_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8974612.1"
                     /translation="MTATLQISRDGDVARVFLNRPDVRNAFNDAVIAELGAAFTGFAA
                     DASLRAVVLGGHGKAFCAGADLSWMRAMADYTWEQNRADAQGLADMLYAIWSCPVPVI
                     GRVHGDCYAGGVGLAAVCDILVAAEGVNFCLSEARLGLLPATIGPYVVKALGEQASRR
                     YFTTAERFDAARAHALGFVHEVVPADALDAKVDELVAALAANGPAAVRACKALVHDVA
                     GRPIDDALRAETAKRIADIRASAEGKEGVQAFLNKRKPSWLGE"
     gene            complement(1158..1817)
                     /locus_tag="VIN75_10380"
     CDS             complement(1158..1817)
                     /locus_tag="VIN75_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974613.1"
                     /translation="MTCLARPPSRLVVIAAALVALLPSVQARNVRLMESVPDAIRVTR
                     THEILGTLDLRFGGDTARGASIVRRDVVVKGVGTTKVKDVGDDDRVRPTDHDLCARAF
                     EDAITQLAFAARGARGTAIVGVISNYEGTVFDDPVHYECHVGALKAAVTLRAQIATGT
                     AAPTPPSQVPVVSPPATPVAPAAPVPVVSPPAPVDVVPPPPPATFQPLPPAPAPAPAP
                     N"
BASE COUNT          268 a          711 c          799 g          247 t
ORIGIN      
        1 gccgccggcc agcgccgcca cgagcgtcca ctgcgcctgg tcgccgccga acacggcggc
       61 cgccagcacc gcgccgccgg gcacgcgcat cagcgtgtgc agcccgtccc agaacgcatt
      121 gacggcgggc accttgtcgg ccacgaactc gatcgtcgcg aggatggcgc tggcgcccag
      181 caccaccggc gactgcagga tgtgcaggcc cggcggcagc gtcacccagc ccatcgcgcc
      241 ggccaggccg gtcaggaaga tgaccaggta caggcgcacg ccgctcgccc agccgagcgc
      301 ggcggcgagc gcgacgaggt gcgcggtgtc gaggtcgttg ttcatcgcgg tcgcggggcg
      361 gtcactcgcc cagccaggac ggcttgcgct tgttgaggaa cgcctgcacg ccttccttgc
      421 cctcggcgct cgcgcggatg tcggcgatgc gcttggcggt ctcggcgcgc agcgcgtcgt
      481 cgatcggccg gccggcgacg tcatgcacca gcgccttgca cgcgcgcacg gcggccgggc
      541 cgttggccgc cagcgcggcg acgagctcgt cgaccttcgc gtcgagcgcg tcggccggca
      601 ccacctcgtg cacgaagccg agcgcgtggg cgcgcgcggc gtcgaaacgc tcggccgtgg
      661 tgaagtagcg gcgcgaggcc tgctcgccga gcgccttgac gacgtacggc ccgatcgtgg
      721 ccggcagcag gcccagcctg gcctcgctga ggcagaagtt gacgccctcg gcggccacga
      781 ggatgtcgca caccgcggcc aggccgacgc cgccggcgta gcagtcgccg tgcacgcggc
      841 cgatgaccgg caccgggcac gaccagatgg cgtacagcat gtcggccagg ccctgcgcgt
      901 cggcgcggtt ctgctcccag gtgtagtcgg ccatcgcgcg catccacgac aggtcggcgc
      961 cggcgcagaa ggccttgccg tggccgccca gcacgaccgc tcgcagcgag gcgtcggccg
     1021 cgaagccggt gaaggccgcg ccgagctcgg cgatcacggc gtcgttgaac gcgttgcgca
     1081 cgtctggccg gttgaggaac acgcgggcga catcgccgtc gcgggagatc tgcagggtgg
     1141 cggtcatggg gggtcggtca gttcggcgcc ggcgcgggcg cgggagccgg cgggagcggc
     1201 tggaaggtgg ccgggggcgg cggcggcacg acgtccaccg gcgcgggcgg cgagaccacg
     1261 ggcacggggg ccgccggcgc gaccggcgtc gccggcggcg acaccacagg cacctgggac
     1321 ggcggcgtcg gggcggcggt gccggtggcg atctgcgcgc gcagcgtcac ggcggccttg
     1381 agcgcgccga cgtggcattc gtagtgcacc ggatcgtcga agaccgtgcc ctcgtagttc
     1441 gagatcacgc cgacgatggc cgtgccgcgc gcgccacggg ccgcgaacgc cagctgcgtg
     1501 atcgcgtcct cgaacgcccg ggcgcacagg tcgtggtcgg tcggacggac gcgatcgtcg
     1561 tcgccgacgt ccttgacctt ggtggtgccc acgcccttga cgacgacatc gcggcgcacg
     1621 atgctggcgc cgcgcgcggt gtcgccgccg aagcgcaggt cgagggtgcc caggatctcg
     1681 tgggtgcgcg tgacacggat cgcgtcgggc accgattcca tcaggcggac gttgcgcgcc
     1741 tgcacggacg gcagcagcgc gacgagcgcg gcggcgatga cgacgaggcg cgagggcggg
     1801 cgggcaaggc aggtcatggt cgagtccttt gcggcaaggc ggccaccaag cgagccgcgt
     1861 ggctgggccg ggcaaatctc ctgccgagcg tcgaggcgat cggtcgccgt gatgggcgtg
     1921 cacccggcgg tgcgatcgcc gtgccggggc gacgggtgcg aagtgcgatc acgggtgctc
     1981 cgccgccggc gcgttgccgc ccagttgcgc ggcgctgccg atgcg
//
LOCUS       DATCRO010000384         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_251440, whole genome shotgun sequence.
ACCESSION   DATCRO010000384 DATCRO010000000
VERSION     DATCRO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251440"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2529)
                     /locus_tag="VIN75_10385"
     CDS             complement(70..2529)
                     /locus_tag="VIN75_10385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8974614.1"
                     /translation="MPRSRSKGRLVVRILLAILALLALLVAVGMWEMHRSLPQVDGQA
                     RLPGLRAPVTIARDSLGTAVVKGANRLDVARGLGYVHAQERFFEMDLTRRSAAGELAE
                     LFGAVALERDRTRRPHRMRATLAARYATLDAGEQAVLQAYADGVNAGLAGLQARPWQY
                     LLLRAEPRPWQPVDSLLVVGEMFWMLQGSSVEDGLERAQLRECVGDDVFDWLEPRGGA
                     WDAALDGSVLEPGAMPGPDRLDLRKPLPPSVALATAARADDAAFLDDEARPDPHEPLV
                     GSNNWAVSGARSITGDAMLANDMHLGLGVPAIWFRAQFEIGDGPDAIRAEGVTLPGVP
                     AMVAGSNGHVAWGFTNSYGQWFDWIRLPAQPDASRMAHVSETIHVKGGDDVAVDVAQF
                     AGLPVLEKDGVHAYALRWSADMAGAYDLGLDRMLGARDVATALAIGAGAGIPHQNMLV
                     ADAHGDIGWTIAGRRLPFGQDPGGAAGADGFGRFVSGASIAAADARAPLHGVAPVIEA
                     PADGLLWTANARTFDQAQAAGPVASGVSARVAGLRPIGDGGYDLGARARQIRDRLRAT
                     PRFDEQSLGAIQFDDEALFLRPWSGRIAGVATGPTARPDVIRLLKDWNGRADADQAGY
                     RLVREVRGRVMDALWTAWTRPLLARPACARRDYKWHARFEYAVEQALDTQPAHLLPPG
                     FATWDAFLLAQVDATVESMTRDGARPLAQATWGEANASHIGHPLARALPVLSRWLDMP
                     SHPQSGDGNMPRVSGPSFGQSERLVVAPGHEERATLAMPGGQSGHPMSPYYGAGHADW
                     VAGRPTPLLAGPVQHVLGLAP"
     gene            complement(2887..4119)
                     /locus_tag="VIN75_10390"
     CDS             complement(2887..4119)
                     /locus_tag="VIN75_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8974615.1"
                     /translation="MTAHRNTLADLRAGRLAGATHLDLRHCGLEDFPREILDLADTLV
                     TLDLSFNRLRALPDELPRLRALRTLFCSSNPFETLPAVLGRCEGLDIVGFKANAITRV
                     PPGSIPANLRWLILSDNRIDAMPATLGDCRGLMKLALAGNRLARLPQGIARLERLELL
                     RLSANRFERPEDALPDGLLALPRLTWLAFAGNPFNAAQEARALAATPIAHIAWHELQL
                     GELLGEGASGRIWSARWHPAGGGDRAVAVKLFKGAVTSDGLPGSEMAACIAADVHAHV
                     IGVEGRIDGHPQGAQGLVMGLIPPRYRSLAGPPSLASCTRDVYAPDVRFTSAQAQAIA
                     RGAGDALAHLHAQGLMHGDFYAHNLLVDDQAHALLGDFGAASFLPEDDGERAEALRRI
                     DRRALDVLVEELAQRTVA"
     gene            4173..5363
                     /locus_tag="VIN75_10395"
     CDS             4173..5363
                     /locus_tag="VIN75_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY8974616.1"
                     /translation="MKHSFLRLALVLGLLSAIGPFAIDMYLPSLPAIGRALRADTHTV
                     QLSLTVFFLSFALSQVLYGPASDMFGRKAPLYAGIALFVAGSVGCALAPDIGTLIAFR
                     FVQGLGGGAPNVITRAVVRDLHTGAEATRLMSLLMLVFSVSPILAPLAGSFIVEAAGW
                     PAVFWTVGFIGLLGLALVAFALEETRPPAQRHGSGLSSTLRAFWLLLRDPHFMGMTGV
                     ASLGVATFFIYLSNSSFVLIEQYGLTPREYGFAFAANAASFIGVSQLTGRLTHRHGLR
                     RVVTVAVAGFALAMVAGAALHLAGATQLATLIVVLMIGFGFLGLVIPTTSVMALDHHG
                     EIAGAASALMGTIQMVAGALVIALMGLVSDGGARAMVIGIAVVAVLTWALAWLSMRGE
                     HPAA"
     gene            complement(5364..6161)
                     /locus_tag="VIN75_10400"
     CDS             complement(5364..6161)
                     /locus_tag="VIN75_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8974617.1"
                     /translation="MTSTALSSRAGVGDLLRDWRQRRRLSQLELAGEADISTRHLSFI
                     ETGRSQPSRSMLLRLAERLDMPLRARNEMLNAAGFAALYAERPLDAPEMSVARRTIDA
                     VLAAHAPFPALAVDRHWTLVASNAAVGALLAGVSAELLQGAVNVLRLSLHPDGLAPRI
                     VNLAEWRAHLLARLQQQVDRSGDAQLVALRAELAAYPSRGEAHSHDLDAIAVPLRLRV
                     DGLAEPLSLISTTTVFGTPVDVTLSELALECFYPADDATRAALQRLA"
     gene            6282..6653
                     /locus_tag="VIN75_10405"
     CDS             6282..6653
                     /locus_tag="VIN75_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8974618.1"
                     /translation="MNATPHAIAERYLATWNEADDRRREALVAALWSPQGRYADPLMA
                     GQGHDGIARMIAAARTQFPGLVFTARGQADGHGPFARFSWSLGPAGGAPVAGGTDVAR
                     LDDAGRVVEVIGFLDAGAGHA"
     gene            6646..7452
                     /locus_tag="VIN75_10410"
     CDS             6646..7452
                     /locus_tag="VIN75_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8974619.1"
                     /translation="MREHDFVTTRSGARLFVRDWGAGAPVLLLPGWAMTSDLWATVML
                     RLDEAGLRAVSFDRRGHGRSSDPGPMDYDTLADDLHDVMAALDLRACTVVAHSGAGGE
                     VLRMIARHGASRVGRLVFVGATLPAMMASESNPGGVPPAAIAAVERQLRDDLDGWIED
                     NAEPFCPGASRRTIAWLAAMVQGASRRAIVAFQHAVAHADFRAEFARLDVPVTIIQGD
                     LDVSAPPALCGERMAALKPDAEYLAWTGVAHGPMVTHPLRLADAIAARAS"
     gene            7705..>9436
                     /locus_tag="VIN75_10415"
     CDS             7705..>9436
                     /locus_tag="VIN75_10415"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEY8974620.1"
                     /translation="MAPTAAELIRQSLEQPDDFWAEQARRIDWQRPFDRVCDTSNPPF
                     VQWFPGGLTNLCHNAVDRHAAADPDRAALTWVSTEVDQERTWTTSQLLREVMTMAAAL
                     QELGVRRGDRVLLYMPMIPEATFAMLACARLGAIHSVVFGGFASASLAARIDDARPLV
                     IVSADAGSRGGKVIPYKPLLDAAIEQSTHKPAHVVMVDRGLAPCERVAGRDVAWSELR
                     GRHADAQVPCTWVDATHTSYTLYTSGTTGRPKGVQRDTGGYAVALAASIALIFDGRPG
                     ETYFCTSDIGWVVGHSYIVYGPLIAGMTTLMVEGLPTQGLGGQPDGGIWWRLVEKYKV
                     SVMFSSPTAIRVLKKQDPALLKRHDLSSLRALFLAGEPLDAPTAEWITQGIGKPVIDN
                     YWQTESGWPILTLLSGVESVRTRLGSPGKAVYGYRAVLLDEATGEELAGADQKGVLAL
                     RYPLPPGCMQTVWGDDDRFVDTYWRSIPNQQVYSTFDWAVRDKDGYFFILGRTDDVIN
                     VAGHRLGTREIEEAVNMHPNVAECAVVGVADPLKGQVATAFVVAKDATGLDEPAARLR
                     LEGEIMKTVDR"
BASE COUNT         1256 a         3444 c         3437 g         1299 t
ORIGIN      
        1 gacccgcccc gcccgaactt gacccgagcg ggcccggggt ccgcgcgaaa agtcgcactg
       61 gacggatgat cagggcgcca ggccgagcac gtgctggaca gggcctgcga gcagcggcgt
      121 gggccggccc gcgacccagt cggcgtgccc cgccccgtag tagggcgaca tcgggtggcc
      181 gctctggccg ccaggcatgg ccagcgtggc gcgctcctcg tggcccggcg cgacgacgag
      241 gcgctcggac tgcccgaacg aaggccccga cacgcgcggc atgttgccgt cgcccgactg
      301 cggatgcgac ggcatgtcca accagcgcga cagcaccggc agcgcgcgcg ccagcggatg
      361 gccgatgtgg ctcgcgttgg cctcgcccca cgtcgcctgc gcgagcggcc gcgcgccgtc
      421 gcgcgtcatc gattccacgg tcgcatcgac ctgcgcgagc aggaaggcgt cccaggtcgc
      481 gaagcccggc ggcagcaggt gcgcgggctg cgtgtcgagc gcctgttcga ccgcgtactc
      541 gaagcgcgcg tgccacttgt agtcgcgcct cgcgcacgcc ggccgcgcca gcagcgggcg
      601 cgtccacgcc gtccacagcg cgtccatcac gcggccgcgc acctcgcgca cgaggcggta
      661 gccggcctgg tcggcgtcgg cgcggccgtt ccagtccttg agcagccgga tgacgtcggg
      721 ccgcgcggtc gggccggtgg cgacgccggc gatgcgcccc gaccacgggc gcaggaacag
      781 cgcctcgtcg tcgaactgga tcgcgccgag cgactgctcg tcgaacctgg gcgtggcccg
      841 caggcggtcg cggatctgcc gggcgcgcgc gccgaggtcg taacctccgt cgccgatggg
      901 ccgcagcccc gcgacgcgcg ccgacacgcc gctggcgacg gggcccgcgg cctgcgcctg
      961 gtcgaaggtg cgcgcattgg ccgtccagag cagcccgtcg gcgggcgcct cgatgaccgg
     1021 cgcgacgccg tgcaggggag cgcgggcgtc cgcggcagcg atgctcgcgc cggaaacgaa
     1081 gcggccgaag ccgtcggccc ccgcggcgcc gccgggatcc tggccgaacg ggagccggcg
     1141 gccggcgatg gtccagccga tgtcgccgtg cgcgtccgcg accagcatgt tctggtgcgg
     1201 gatgcccgcg cccgcgccga tcgcgagcgc cgtggcaacg tcgcgcgcgc cgagcatccg
     1261 atccagcccg aggtcgtacg cgccggccat gtcggcggac cagcgcagcg cgtatgcgtg
     1321 cacgccgtcc ttctccagca ccggcaggcc ggcgaactgc gcgacgtcga cggccacgtc
     1381 gtcgccgccc ttgacgtgga tcgtctcgga cacgtgcgcc atgcgggacg cgtcgggttg
     1441 cgccggcaac cggatccagt cgaaccactg cccgtacgaa ttcgtgaagc cccaggccac
     1501 atggccgttc gagccggcca ccatcgccgg cacgccgggc aacgtgacgc cctcggcgcg
     1561 gatcgcgtcc gggccgtcgc cgatctcgaa ctgcgcgcgg aaccagatcg ccggcacgcc
     1621 cagccccagg tgcatgtcat tggccagcat cgcgtcgccg gtgatgctgc gcgcgccgga
     1681 gacggcccag ttgttgctgc cgaccagcgg ctcgtgcgga tcgggccgtg cctcgtcgtc
     1741 caggaacgct gcatcgtcgg cgcgcgccgc cgtcgccaac gcgaccgagg gcggcagcgg
     1801 cttgcgcagg tcgagccggt cgggcccggg catcgcgccg ggctcgagca ccgagccgtc
     1861 gagcgccgcg tcccaggcgc cgccgcgcgg ctccagccag tcgaacacgt cgtcgccgac
     1921 gcactcgcgc aattgcgcgc gctccaggcc gtcttcgacg ctcgagccct gcagcatcca
     1981 gaacatctcg ccgaccacca gaagcgagtc caccggctgc cagggccgcg gctcggcgcg
     2041 cagcagcagg tattgccagg gtctcgcctg caggccggcg aggcccgcgt tgacgccgtc
     2101 ggcgtaggcc tgcagcacgg cctgctcgcc cgcgtccagc gtggcgtaac gcgcggcgag
     2161 cgtggcgcgc atgcggtgcg ggcggcgcgt cctgtctcgc tcgagcgcga ccgcgccgaa
     2221 cagctccgcc agctcgccgg cggcggagcg ccgcgtcagg tccatctcga agaagcgctc
     2281 ctgcgcatgc acgtagccca ggccgcgcgc gacatcgagc cggttcgcgc ccttgacgac
     2341 ggccgtgccg agcgaatcgc gcgctatcgt caccggcgcc cgcaaccccg gcaggcgcgc
     2401 ctggccgtcg acctgcggca ggctgcgatg catctcccac atcccgacgg ccaccagcaa
     2461 tgccagcagc gcgaggatcg cgagcaggat ccggacgacc aggcgtccct tcgaacgcga
     2521 acgcggcatg cggtctccct tcgtgcgggc gattctagtg tcgcgatcgg gaagttcgtt
     2581 gaacaggtcg cgagcgcaga ccaccagggc ctgttcacac tgaatgcgtg cccgcgtgac
     2641 ctccagaaag cccgccttca aggcgcgggc gcgccgacgt ggtggcgcca cggcaagcgg
     2701 ccgcaacgcc gaaggcgggc tttctggagg atcacccgca gggccggctg cctgcgaggc
     2761 cccaggcgtc gttggccgct ccttgcgggc gtccagcccg ccgcgtcgcg tccgcctagc
     2821 cctggggcct cgcaggcagc cggcgcgggc acgcattcag tgtgaacagg ccctagcgtg
     2881 caacgctcag gcgacggtgc gctgcgccag ctcctccacc agcacgtcca gcgcgcgccg
     2941 gtcgatgcgc ctgagcgcct cggcgcgctc gccgtcgtcc tcgggcagga acgacgcggc
     3001 gccgaagtcg cccagcagcg cgtgcgcctg gtcgtcgacc agcaggttgt gcgcgtagaa
     3061 gtcgccgtgc atcaggcctt gcgcgtgcag gtgcgcgagc gcgtcgcccg cgccgcgcgc
     3121 gatcgcctgc gcctgagccg atgtgaagcg cacgtcgggc gcatagacgt cgcgcgtaca
     3181 gctggccagg ctcggcgggc cggccaggct gcgatagcgc ggcgggatca ggcccatcac
     3241 caggccctgc gcgccctgcg ggtgcccgtc gatgcggccc tcgacgccga tcacgtgcgc
     3301 gtggacgtcg gccgcgatgc acgccgccat ctcgctgccg ggcaggccgt cgctggtcac
     3361 cgcgcccttg aacagcttga cagccacggc gcggtcgccg ccgcccgccg gatgccagcg
     3421 cgccgaccag atgcggcccg aggcgccctc gcccagcagc tcgcccagct gcagctcgtg
     3481 ccacgcgatg tgcgcgatgg gcgtggcggc cagcgcgcgc gcctcctgcg cggcgttgaa
     3541 cgggttgccg gcgaaggcga gccaggtcag gcgcggcagc gcgagcaggc cgtcgggcag
     3601 cgcgtcctcc gggcgctcga agcggttggc cgacaggcgc agcagctcca ggcgttcgag
     3661 gcgcgcgatg ccctgcggca gccgcgccag gcggttgccg gccagcgcga gcttcatcag
     3721 gccgcggcag tcgcccagcg tcgcgggcat cgcgtcgatg cggttgtcgc tgaggatcag
     3781 ccagcgcagg ttcgccggga tcgacccggg tggcacgcgc gtgatcgcgt tggccttgaa
     3841 gccgacgatg tccaggccct cgcatcggcc cagcaccgcg ggcagcgtct cgaacgggtt
     3901 ggacgagcag aacagcgtgc gcagcgcacg cagccgaggc agttcgtcgg gcagcgcgcg
     3961 caggcggttg aacgacaggt ccagcgtgac gagggtgtcg gccaggtcca ggatctcgcg
     4021 cgggaagtcc tccaggccgc agtggcgcag gtcgaggtgc gtggcgccgg cgaggcggcc
     4081 ggcgcgcagg tcggcgagcg tgtttcggtg agcggtcatc tgcgccggat tgtcgccgtc
     4141 cctatacttc gcggccattt ccccgaagtt ccatgaagca ttccttcctg cgcctggccc
     4201 tcgtgctggg cctgctctcg gccatcgggc cgttcgcgat cgacatgtac ctgccctcgc
     4261 tgccggccat cggccgcgcg ctgcgggccg acacgcacac ggtgcagctg agcctgaccg
     4321 tgttcttcct gtcgttcgcg ctcagccagg tgctgtacgg tccggcgtcg gacatgttcg
     4381 gccgcaaggc gccgctgtac gccggcatcg cgctgttcgt ggccggcagc gtgggctgcg
     4441 cgctcgcacc cgacatcggc acgctgatcg cgttccgctt cgtgcagggc ctgggcggcg
     4501 gcgcgcccaa cgtgatcacg cgcgccgtcg tgcgggacct gcacaccggc gcggaggcca
     4561 cgcggctgat gtcgctgctg atgctcgtgt tcagcgtgag cccgatcctc gcgccgctgg
     4621 cgggcagctt catcgtcgag gccgccggct ggccggccgt gttctggacg gtgggcttca
     4681 tcggtctcct cggcctcgcc ctggtggctt tcgcgctgga ggagacgcgg ccgcccgcgc
     4741 agcgccacgg cagcggcctc tccagcacgc tgcgcgcgtt ctggctgctg ctgcgcgacc
     4801 cgcacttcat gggcatgacc ggcgtcgcct cgctgggcgt ggcgaccttc ttcatctacc
     4861 tgtcgaactc gtcgttcgtg ctgatcgagc agtacggcct gacaccgcgc gaatacggtt
     4921 tcgcgttcgc ggccaacgcg gcgtcgttca tcggcgtctc gcagctgacg ggccgcctga
     4981 cgcaccgcca tggcctgcgc cgcgtggtga cggtggccgt ggccggcttc gcgctggcga
     5041 tggtcgcggg cgccgcgctg cacctggcgg gcgccacgca gctggccacg ctgatcgtcg
     5101 tgctgatgat cggcttcggc ttcctgggcc tcgtgatccc gacgacgtcg gtgatggcgc
     5161 tcgaccatca tggcgagatc gccggcgccg cctcggcgtt gatggggacg atccagatgg
     5221 tggccggcgc gctggtcatc gcgttgatgg gcctggtcag cgacggtggc gcgcgggcga
     5281 tggtgatcgg catcgcggtg gtggcggtgc tgacgtgggc gctggcgtgg ctctcgatgc
     5341 gcggcgagca ccccgcggcc tgatcacgcg aggcgctgca aggccgcccg cgtcgcgtcg
     5401 tcggccggat agaagcactc gagcgcgagc tccgacagcg tcacgtccac cggcgtgccg
     5461 aacaccgtcg tcgtgctgat cagcgacagc ggctcggcca gcccgtccac gcgcaggcgc
     5521 aggggcacgg cgatggcgtc gaggtcgtgc gagtgcgcct cgccgcgcga cgggtaggcc
     5581 gcgagctccg cacgcagcgc gacgagctgc gcgtcgcccg agcggtcgac ctgctgctgc
     5641 aggcgtgcga gcaggtgcgc gcgccactcg gccaggttga cgatgcgcgg cgcgaggccg
     5701 tccggatgca ggctcaggcg cagcacgttg acggcgccct gcagcagctc ggccgacacc
     5761 ccggccagca gcgcgcccac cgcggcgttg ctcgccacca gcgtccagtg gcgatcgacc
     5821 gcgagcgcgg gaaacggcgc gtgcgcggcc agcaccgcat cgatggtgcg gcgcgcgacc
     5881 gacatctccg gtgcatccag cggccgctcc gcgtagagcg cggcgaagcc ggcggcgttg
     5941 agcatctcgt tgcgcgcacg cagcggcatg tccaggcgct cggccaggcg cagcagcatg
     6001 ctgcggctgg gctgcgaacg gccggtctcg atgaagctca ggtggcgcgt ggagatgtcc
     6061 gcctcgccgg cgagctcgag ctggctgagg cggcggcgct ggcgccagtc gcggagcagg
     6121 tcgccgacgc cggcgcggga cgagagggcg gtcgatgtca tggccgcagg atagcgccgc
     6181 gccggcacgc gtcccatgac ctggcaggta atggacgctg cgcacccggc tcgcgacgat
     6241 gacgtccatc gcagcatcgc gatcacttca aggagaccga catgaacgcc accccgcatg
     6301 ccatcgccga acgctacctg gccacctgga acgaggccga cgaccgccgc cgcgaggcgc
     6361 tcgtcgccgc gctgtggagc ccgcagggac gctacgcgga cccgctgatg gccggccagg
     6421 gccatgacgg catcgcgcgc atgatcgccg cggcccgcac gcagttccca ggcctcgtgt
     6481 tcacggcgcg cggccaggcc gacgggcacg ggccgttcgc gcgcttctcg tggtcgctgg
     6541 gcccggccgg cggcgcgccg gtggccggtg gcaccgacgt cgcgcggctc gacgacgccg
     6601 gccgcgtcgt cgaggtcatc ggcttcctgg acgcgggagc cggccatgcg tgaacacgac
     6661 ttcgtcacca cgcgctcggg cgcccgcctg ttcgtgcgcg actggggtgc cggcgccccg
     6721 gtgctgctgc tgcccggctg ggcgatgacg tccgacctgt gggccaccgt catgctgcgg
     6781 ctggacgagg ccggcctgcg cgccgtctcg ttcgaccgcc gcggccacgg ccgctccagc
     6841 gacccggggc cgatggacta cgacacgctg gccgacgacc tgcacgacgt gatggcggcg
     6901 ctggacctgc gcgcctgcac ggtcgtcgcg cactcgggcg ccggcgggga ggtgctgcgc
     6961 atgatcgcgc gccacggcgc ctcgcgcgtc ggccgcctcg tgttcgtggg cgcgacactg
     7021 ccggcgatga tggcgtcgga gtccaacccc ggtggcgtgc cgcccgcggc catcgcggcg
     7081 gtcgagcgcc agttgcgcga cgacctcgac ggctggatcg aggacaacgc cgagccgttc
     7141 tgcccgggcg cgtcacgtcg caccatagcc tggctcgccg ccatggtgca gggcgcgtcg
     7201 cgccgtgcga tcgtcgcgtt ccagcatgcg gtcgcgcacg ccgacttccg cgccgagttc
     7261 gcgcgcctgg acgtgccggt caccatcatc cagggcgatc tggacgtctc cgccccgccc
     7321 gcgctgtgcg gcgagcgcat ggccgccctc aagccggacg cggagtacct cgcctggacg
     7381 ggcgtcgccc acggcccgat ggtgacgcac ccgctgcggc tggccgacgc gatcgcggcg
     7441 cgggcaagct gagcgtccgg ctcggcgtca ggtcttgcca tttgcctcgc ccccgttggg
     7501 gtcaggtctt gaatgttgcg gggtcaggtc ttgaatcgtg cagtgcgctc gcgaagactt
     7561 cgctgcgacg cccggaagtc gcaattcgtg caccttcgac cgcgatgcgg taccgttagc
     7621 tggtacgcaa gacctgaccc cgcaacattc aagacctgac cccgtgtttc gccgcagagg
     7681 gcaggacctg acaccggaga cagcatggcc ccgaccgccg cggagctcat ccgccaatcg
     7741 ctcgaacagc ccgacgactt ctgggccgaa caggcccgcc gcatcgactg gcagcgcccg
     7801 ttcgatcgcg tgtgcgacac gtcgaacccg ccgttcgtgc agtggttccc gggcgggctg
     7861 accaacctgt gccacaacgc ggtcgaccgc catgccgcgg cggatcccga ccgcgcggcg
     7921 ctcacgtggg tctcgacgga ggtcgaccag gaacgcacct ggacgacctc gcagctgctg
     7981 cgcgaggtga tgacgatggc cgccgcgctg caggagctcg gcgtgcgccg gggcgaccgc
     8041 gtgctgctgt acatgccgat gatccccgag gccacgttcg cgatgctggc ctgcgcgcgg
     8101 ctgggcgcga tccactccgt ggtgttcggc ggcttcgcca gcgcgagcct tgccgcgcgc
     8161 atcgacgatg cgcggcccct ggtgatcgtc agcgccgacg cgggttcgcg cggcggcaag
     8221 gtgatcccgt acaagccgct gctggacgcg gcgatcgagc agtcgacgca caagccggcg
     8281 cacgtcgtga tggtcgaccg cggcctcgcg ccctgcgagc gcgtcgccgg ccgtgacgtc
     8341 gcctggtccg agctgcgcgg gcgccacgcc gacgcgcagg tgccgtgcac gtgggtcgac
     8401 gccacgcaca ccagctacac gctctacacc tccggcacca cgggccggcc caagggcgtg
     8461 cagcgcgaca ccgggggcta cgccgtcgcg ctggcggcct cgatcgcgct gatcttcgac
     8521 ggccggcccg gcgagaccta cttctgcacc agcgacatcg gctgggtcgt gggccacagc
     8581 tacatcgtct acggaccgtt gatcgccggc atgacgacgt tgatggtcga aggcctgccc
     8641 acgcagggcc tgggcgggca gccggacggc ggcatctggt ggcggctggt cgagaagtac
     8701 aaggtctcgg tgatgttctc gtcgccgacc gcgatccgcg tgctcaagaa gcaggatccg
     8761 gcgctgctca agcgccacga cctgtcgtcg ctgcgcgcgc tgttcctcgc cggcgagccg
     8821 ctggacgcgc ccaccgccga atggatcacc caggggatcg gcaagcccgt catcgacaac
     8881 tactggcaga ccgagagcgg ctggccgatc ctgacgctgc tctcgggcgt cgaatccgtc
     8941 cggacgcgcc tgggctcgcc cggcaaggcg gtgtacggct accgcgccgt gctgctggac
     9001 gaggccaccg gcgaggaact ggccggcgcc gaccagaagg gcgtgctcgc gctgcgctac
     9061 ccgctgccgc ccggctgcat gcagacggtc tggggcgacg acgaccgctt cgtcgacacg
     9121 tactggcgca gcatcccgaa ccagcaggtg tactccacct tcgactgggc ggtgcgcgac
     9181 aaggacggct acttcttcat cctcggccgc accgacgacg tcatcaacgt ggccggccac
     9241 cgcctgggga cgcgcgaaat cgaggaggcg gtcaacatgc atccgaacgt cgccgaatgc
     9301 gcggtggtcg gggtcgccga tccgctgaag gggcaggtcg cgaccgcgtt cgtcgtcgcg
     9361 aaggacgcga ccggcctcga cgagccggcc gcgcggctca ggctcgaagg cgagatcatg
     9421 aagacggtcg accgcc
//
LOCUS       DATCRO010000385         5007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_533060, whole genome shotgun sequence.
ACCESSION   DATCRO010000385 DATCRO010000000
VERSION     DATCRO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533060"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIN75_10420"
     CDS             complement(<1..194)
                     /locus_tag="VIN75_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974621.1"
                     /translation="MITPSLNINLPSASTASTAAKAVVPPPASAATAAPAPLPSAPPA
                     PSATVTLSPQALAASHAASLP"
     gene            422..1906
                     /locus_tag="VIN75_10425"
     CDS             422..1906
                     /locus_tag="VIN75_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8974622.1"
                     /translation="MERTKAGASLRRRITLLALACVALALCAGVRLLQVDGQSAVAMV
                     CLALGGVAVALALSLQRQLAGSLQPLQASVHAMKDGDLSRQAADVRGAEFGAIAASLD
                     DMNANLSALVADIRSEATFVSQASETLAAGTTELAQRTERQAASLEQTSASVQDLSGS
                     VKQNALDARAVEQLASRVHEMAESGGSRMREAVETMHAIRASAQRVHDIIGVIDGIAF
                     QTNILALNAAVEAARAGESGRGFAVVAAEVRTLAVRSGDAAREIKALIAASSEHVASG
                     TSRIDEVGALLANVVSGIGEVAGNVRAITLAAEQQSIGLGQMSEALHGLDGITQENAA
                     MVEQTSTASAGLGERAHKLARTVGTFRLRQGTADEAHALVTRAVALYRRRGTASLAEI
                     TEAASGFADRDMYVFAWDRGLVYHAFAGKPHNVGKTGAQILGTDVTQLTRDVWAAAAQ
                     GGGWVDYDFLNPSTGQIAPKRSFIVPVTDDLVLGCGIYRTVQRH"
     gene            complement(2030..2425)
                     /locus_tag="VIN75_10430"
     CDS             complement(2030..2425)
                     /locus_tag="VIN75_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974623.1"
                     /translation="MITRHLITSATLALAFFGSGAFAADEGCRTVANGVQICDVHTGH
                     ERSRDAVAAEIHAKADTSTGCRTNANGVQICDVPTGRERSREAVVAELHGAAGYTIEG
                     CRTVANGVQKCDVPSPYTARNETAIAAAH"
     gene            complement(2625..3632)
                     /locus_tag="VIN75_10435"
     CDS             complement(2625..3632)
                     /locus_tag="VIN75_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8974624.1"
                     /translation="MATIKDVAKLAGVGVGTASRVISGKGSFSEAAAARVEAAARQLG
                     FRPSAIARALSLQSTGTIGVFVPDFKGPFYGPLLDAIDAELRQHDRHMVAANGCGDED
                     TRQQSLDGVRFLIERECDGIIMCSNALRDSDFAALRDDFANLAIVNRDVKGMKAHCFT
                     VDHRAAGRAAAQALLAHGHRRIAVITGPAIAGDNKQRLQGLFDRLGEAGIAADSVMVE
                     EGDFSAPSGWAACERLVARKPKITALFCANDQMAMGAISFLGHHGIRVPQQWSVMGYD
                     DSDVAAFLSPRLTSVRIPIAEMALNACRMLINTRFGTDLPVSRKFVPTVVMRDSLAKA
                     P"
     gene            complement(3646..4692)
                     /locus_tag="VIN75_10440"
     CDS             complement(3646..4692)
                     /locus_tag="VIN75_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974625.1"
                     /translation="MNVSIQQLAVHMGGTPVLEKLDLDIVDGEFLVLLGPSGCGKSTL
                     LHAIAGLIDVEAGAVHIGGRDVTWADPKDRAIGMVFQSYALYPTMNVERNMSFGLRVA
                     GTPKAEISQRVARAARMLQLEPLLSRKPAQLSGGQRQRVAIGRALVREAQVFLFDEPL
                     SNLDAKLRTELRRELKALHRELGATMIYVTHDQVEAMTLATRVAVMRGGRIQQVGTPG
                     EVYARPANMFVAGFLGSPSMNFIEGEVSAGADAPGVAAILGVRPEHLALHPAGGVHPQ
                     AEVTLVEAMGAHEVIWLDMQGQRLSAIAPAAGAHQIGDRVGVQMDLDRASVFDPVSQE
                     RIDLHAGAFRHRAR"
     gene            complement(4806..>5007)
                     /locus_tag="VIN75_10445"
     CDS             complement(4806..>5007)
                     /locus_tag="VIN75_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8974626.1"
                     /translation="IGLVFAGHDWLPMTVQLNNIINTTTGERLYNVNMAATLLTSLVP
                     LTVYLVSGRWFVRGIAAGAVKG"
BASE COUNT          697 a         1719 c         1880 g          711 t
ORIGIN      
        1 ggcaacgacg ccgcatggct ggccgccagt gcctgcgggc tcagcgtcac ggtcgcgctg
       61 ggcgccggcg gcgcgctcgg cagcggggcc ggcgccgcgg tcgcggcgct ggcggggggc
      121 gggacgacgg ccttggccgc cgtcgacgcg gtgctggccg acggcaggtt gatattgagg
      181 ctgggggtga tcatgcggcg gtccttccgc gcgaagcgtt ctcgcgccgg gtcagtctag
      241 gcaggcgcgt ggaatccgac cgctcgatca gtgccgggaa tcgggtgcat tcccgccgcc
      301 gctatcgatg gatggctcgg aatagcggcc ggttgccgcc gttgttgcgc gctttcccgg
      361 gcgaccgctt gcccagaatg acgggatgga gccgcaccgg cggccgccac gaggacatgg
      421 gatggaacga acgaaggccg gcgcgtcgtt gcgccgtcgg atcacgctgc tggcgctcgc
      481 ctgcgtcgcg ctggcgctgt gcgcgggcgt gcggctgctg caggtcgacg ggcagtccgc
      541 cgtcgcgatg gtgtgcctgg cgctcggcgg cgtggccgtc gcgctcgcgc tgtcgctgca
      601 gcgccagctc gccggatcgc tccagccgct gcaggccagc gtgcacgcga tgaaggacgg
      661 cgacctgtca cgccaggcgg ccgacgtgcg cggcgccgag ttcggcgcca tcgccgcgtc
      721 gctcgacgac atgaacgcga acctgtccgc gctggtggcc gacatccgca gcgaggcgac
      781 cttcgtgtcg caggccagcg agacgctcgc ggccggcacc accgagctcg cccagcgcac
      841 cgaacgccag gccgcgagcc tggagcagac ctcggccagc gtgcaggacc tcagcggttc
      901 cgtgaagcag aacgcgctcg acgcgcgcgc cgtcgaacag ctcgcgagcc gcgtgcacga
      961 gatggccgag tcgggcggca gccgcatgcg cgaggccgtc gagacgatgc acgcgatccg
     1021 cgccagcgcg cagcgcgtgc acgacatcat cggcgtcatc gacggcatcg cgttccagac
     1081 caacatcctc gcgctcaatg ccgcggtcga ggccgcccgc gccggcgaga gcggacgcgg
     1141 cttcgccgtc gtggccgccg aggtgcgcac gctggccgtg cgcagcggcg acgcggcgcg
     1201 cgagatcaag gcgctgatcg ccgcgtccag cgagcacgtg gcctcgggga cgtcgcgcat
     1261 cgacgaggtg ggcgcgctgc tggccaatgt cgtctcgggc atcggcgagg tcgcgggcaa
     1321 cgtgcgcgcg atcacgctgg cggccgagca gcagagcatc ggcctggggc agatgtccga
     1381 ggccttgcac ggcctggacg gcatcacgca ggagaacgcg gcgatggtcg aacagacctc
     1441 gacggcgtcg gccggcctgg gcgagcgcgc gcacaagctc gcgcgcaccg tgggcacctt
     1501 ccgcctgcgc cagggcacgg ccgacgaggc gcatgcgctg gtgacccggg ccgtcgcgtt
     1561 gtaccggcgt cgcggcaccg cgagcctggc cgagatcacc gaggccgcca gcggcttcgc
     1621 ggaccgcgac atgtacgtgt tcgcgtggga tcgcgggctc gtctaccacg cgttcgcggg
     1681 caagccgcac aacgtcggca agacgggcgc gcagatcctg ggcaccgacg tcacgcagct
     1741 cacgcgcgac gtgtgggccg cggccgcgca gggcggcggc tgggtcgact acgacttcct
     1801 caatccgtcc accggccaga tcgcgcccaa gcgctccttc atcgtgccgg tgacggacga
     1861 cctggtgctg ggctgcggca tctacaggac ggtgcagcgg cactgacggc gccgcccgcg
     1921 ggatgcgggc aagcgtgggg cggcgggttc aacttcatgc acgccgccgc ggcgtcggtc
     1981 accgcatcga cgtcaggaag cggtcgtcag gggcgtctcc gcgcggggtt cagtgcgccg
     2041 cggcgatcgc cgtctcgttg cgcgcggtgt acggcgacgg cacgtcgcac ttctgcacgc
     2101 cgttggcgac cgtgcggcag ccctcgatcg tgtagccggc ggcgccgtgc agttcagcga
     2161 cgacggcctc gcggctgcgt tcgcgaccgg tgggcacgtc gcagatctgc acgccgttgg
     2221 cgttcgtgcg gcagccggtg gaggtgtcgg ccttcgcgtg gatctccgcg gccacggcgt
     2281 cgcggctgcg ttcgtggccg gtgtgcacgt cgcagatctg cacgccgttg gccaccgtgc
     2341 ggcagccctc gtcggcggcg aatgcgccgc tgccgaagaa ggcgagggcg agcgtggcgg
     2401 aggtgatgag gtgtcgggtg atcatggtct tctcgtggtt gtcctgaggg aggcgcgcgg
     2461 cgtccgtgcc ggaggggggt cgcgtcgtcg tggaaccact gtacgaagac ccgcggcgcg
     2521 gaaaaagcgc tgccgccgga attcaccatt gctgaaaacc gaggtccaag gtcattcgcg
     2581 ccgcgccgtg cgcggcgacg cgcgccatgg caggcgcgtg gcgctcaggg cgccttggcc
     2641 agcgagtcgc gcatcaccac cgtgggcacg aacttgcgcg agaccggcag gtcggtgccg
     2701 aagcgggtgt tgatcagcat gcggcaggcg ttgagcgcca tctcggcgat cgggatgcgc
     2761 acgctggtca ggcgcggcga gaggaaggcg gcgacgtccg agtcgtcgta gcccatcacc
     2821 gaccactgct gcggcacgcg gatgccgtgg tggcccagga agctgatcgc gcccatggcc
     2881 atctggtcgt tggcgcagaa cagcgcggtg atcttcggct tgcgcgccac caggcgctcg
     2941 caggcggccc agccggaggg cgcgctgaag tcgccctcct ccaccatcac ggagtccgcc
     3001 gcgatgccgg cctcgccgag gcggtcgaac aggccctgca ggcgctgctt gttgtcgccg
     3061 gcgatggccg gaccggtgat gaccgcgatg cgacggtggc cgtgtgccag cagcgcctgg
     3121 gcggcggcgc ggccggcggc gcggtggtcg acggtgaagc agtgcgcctt catgcccttg
     3181 acgtcgcggt tgacgatggc caggttggcg aagtcgtcgc gcagcgccgc gaagtcggag
     3241 tcgcgcagcg cgttgctgca catgatgatg ccgtcgcact cgcgctcgat caggaagcgc
     3301 acgccgtcga gcgactgctg gcgggtgtcc tcgtcgccgc agccgttggc ggccaccatg
     3361 tggcggtcgt gctggcgcag ctcggcgtcg atcgcgtcca gcagcgggcc gtagaacggg
     3421 cccttgaagt cggggacgaa cacgccgatg gtgccggtgc tctgcaagga cagggcgcgc
     3481 gcgatcgcgc tcgggcggaa gcccagctgg cgcgccgccg cctcgacgcg cgccgcggcg
     3541 gcctccgaga acgagccctt gccgctgatg acgcgcgagg cggtgcccac gcccacgccg
     3601 gcgagcttcg cgacgtcctt gatcgttgcc atggccgaga cgatatcagc gggcgcggtg
     3661 ccggaacgcg cccgcgtgca gatcgatgcg ttcctgcgac accgggtcga acacgcttgc
     3721 gcgatccagg tccatctgca cgcccacgcg gtcgccgatc tggtgcgcgc cggcggccgg
     3781 cgcgatggcc gacaggcgct ggccctgcat gtccagccag atcacctcgt gcgcacccat
     3841 cgcctcgacg agggtcacct cggcttgggg atggacgccg ccagccggat gcagcgcgag
     3901 gtgctcgggc cgcacgccga ggatggccgc gacgcccggc gcgtcggcac cggccgacac
     3961 ctcgccctcg atgaagttca tgctcggcga acccaggaag cccgccacga acatgttggc
     4021 cggccgtgca tacacctcgc cgggcgtgcc gacctgctgg atgcggccgc cgcgcatcac
     4081 ggccacgcgc gtggccagcg tcatcgcctc gacctggtcg tgcgtgacgt agatcatcgt
     4141 cgcgccgagc tcgcggtgca gggccttgag ctcgcgccgc agctcggtgc gcagcttggc
     4201 gtcgaggttg gacagcggct cgtcgaacag gaacacctgc gcctcgcgca ccagggcgcg
     4261 cccgatcgcc acgcgctggc gctggccgcc cgacagctgc gccggcttgc gcgagagcag
     4321 cggctcgagc tgcagcatcc tggcggcgcg cgccacgcgc tgcgagatct ccgccttggg
     4381 cgtgccggcc acgcgcagcc cgaacgacat gttgcgctcg acgttcatcg tcggatagag
     4441 cgcgtacgac tggaacacca tgccgatcgc gcggtccttc ggatcggccc aagtcacgtc
     4501 gcgcccgccg atgtgcacgg cgcccgcctc gacgtcgatc aggcccgcga tggcgtgcag
     4561 cagcgtggac ttgccgcagc ccgacggccc cagcagcacg aggaactcgc cgtcgacgat
     4621 gtcgaggtcg agcttctcca gcacgggcgt gccgcccatg tgcacggcga gttgttggat
     4681 ggagacgttc atttcagtag cctgcgaagc agggatttct cgacgcccgc gagcgcgcgg
     4741 ccgctccgga gcgagcgggc ttgaggccct cggggccggg gagcggagcg agccgggctg
     4801 tcatttcagc cctttaccgc gccggcggcg atgcctcgaa cgaaccagcg gccggacacg
     4861 aggtacacgg tcagcggcac cagcgacgtc agcagcgtgg ccgccatgtt gacgttgtag
     4921 agccgctcgc cggtggtggt gttgatgatg ttgttcagct gcacggtcat cggcagccag
     4981 tcgtggccgg cgaacacgag gccgatc
//
LOCUS       DATCRO010000386         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1005488, whole genome shotgun sequence.
ACCESSION   DATCRO010000386 DATCRO010000000
VERSION     DATCRO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005488"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VIN75_10450"
     CDS             <1..1202
                     /locus_tag="VIN75_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974627.1"
                     /translation="FLYALTLSSTDIVPVEAVRMYKERLSVTPVGTGPFMATGPLDRK
                     GTLRLVRNPGYYRTYPSTGAPGDAGKGLLKDAGRRLPFVDVLELPLIEEPQPAALKFL
                     RGELDRRELDRANFTRLVARAPDGTLRPADGYAGKFTLASEPGGNAIYISVNMRDPVL
                     GRNKALRQAIAAAIDPQSDIDVLWNGRGIAMQTLVPVELAGSAHDTAAPPHKRDVALA
                     RKLLAQAGFPDGKGLPPLAMRFPSSDAATRNEFDFFKAQLAAIGVQLTGDFDDTPTFT
                     KALDAGNFQLASYSYYADYPDAEDFYQMLYGRNVSPGPNYGAFANAAYDKAYEASRYM
                     VNGPKRFELFRTMDAIVRDEVPLIPLRESLRVDTVQPWVANYKRNVFTTEMQFMRVDM
                     AAKRKGP"
     gene            1205..2986
                     /locus_tag="VIN75_10455"
     CDS             1205..2986
                     /locus_tag="VIN75_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974628.1"
                     /translation="MRLVSPAIARVLACAALAGALTAPAHAQDKSVLTVPRISRFETL
                     DPQRGFGQTETQVVDQVYSHLVTYAYLERPYKLEPDLLEAMPTLSPDQLTLTFHLRPG
                     VRFVDNACFPGGKGRELTSDDVLYSLKRYADGNVNTKSWFAMQGAVVGLDDYRAATIK
                     AGPSADLTSRDVAGLRKVDAHTFTIRLTHPNALFLYALTMSPTSIVPREAVQAYKDRF
                     ALNPVGTGPFMATQQVDRKGVIRLVRNPNYYRTYPATGEPGDAAAGLLKDAGKRLPLV
                     DELQMPLIEEQQPAALKFLHGDLDWEPMDRASFARMVRRNPDGTFRLAPEFAGKFAIH
                     AVPSLETDYLMVNMRDPVLGRNKLLRQALAAAIDARAIVDVLWNGRGRPLGSLVPWDI
                     PGNERDTGAVARPHDLAQARKLLAQAGYPDGQGLPPLTISFFQTHVAAHNEFDLLKAQ
                     LAAVGVQAKGVFMDQPTFTKAAENGNYQLASFGWEADYPDAEDFYQLLYGPNAPPASN
                     WTGYANPAYDKAYETARFMANGPERLAQMRIMNALIADDVPLIVTFDPLKFGITQAWV
                     GNFKRNLLIQEHLFLSVDMARKRKGLQ"
     gene            3006..3938
                     /locus_tag="VIN75_10460"
     CDS             3006..3938
                     /locus_tag="VIN75_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974629.1"
                     /translation="MGAYIVRRLLQAVPTVFGVALLTFLLFDTFGPDPVRTALGNHAT
                     AEAMANLRHQWGLDKPLPLQFLDFLRQIATFDYGKSFVTGDDLGTQLKDGALVSLSVT
                     MPPFLFGGMVNIAIALFIARHRDGVVDKAARALFIAAMSVSALVWVLALQYLLAFKLD
                     WFPINGYEGGWSAVKYLGLPWLIQLLLLMGPDIRLYRALFLADMNADWVRTARAKGAS
                     ESRVLLRHVLPNAWIPIITQNVTTIPFLILGSFLMERFFSIPGIGNLTIDALSRGDLP
                     ILKAVTVLGALALVVFNLVNDLLYAVADPRVRLQ"
     gene            3935..4816
                     /locus_tag="VIN75_10465"
     CDS             3935..4816
                     /locus_tag="VIN75_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8974630.1"
                     /translation="MSALPDLVAATPAPRPAAREALAALWARPHIRGCLIVVALYALV
                     AACGWLHVLPDAEAAVGGAQDPPSWHLATLLGTDLLGRSVLWRVLAGAQTAVTIGLAT
                     TALAVPFGTALGLAAGYFGGWVDAVVNWVYSVVVSVPDILLITAISYAMGKGLTSMCV
                     AIAATSWVGIMRLVRGEVLRHKGSDYVLAARSVGAGAARVMFSEILPNVVHVAIVTTT
                     LVVLAAVKGEVILTWLGLGVQDGASWGLLIAGAAQDLTNDIWGPLAGTVTAMFLLIYS
                     LSAIGDALRDVLDPRVA"
     gene            complement(4818..5099)
                     /locus_tag="VIN75_10470"
     CDS             complement(4818..5099)
                     /locus_tag="VIN75_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974631.1"
                     /translation="MNAANNSPLKAHRTPDMSIAWEAQPSLQHLVVRKDDAADVPTWC
                     ETMPVSLDAMNAPVVYREPLEGLSVRDIDEPEIFNVFFGDSGAPLRRAA"
     gene            complement(5551..6021)
                     /locus_tag="VIN75_10475"
     CDS             complement(5551..6021)
                     /locus_tag="VIN75_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEY8974632.1"
                     /translation="MQFESLVTLLEAGGARFRVIEHPAEGRSDLVAQIRGTAPGQGAK
                     AMLCRSKDGDPAYALVVLPGDRKLDFKRVAQALGMKKATLASPEEAMATTGCAIGAIP
                     PFVDGPSQRLVVDPALVEQFAEIAFNAGRLDRSVVLDSADYVRIARPMLVPVSA"
BASE COUNT          932 a         2170 c         2146 g          946 t
ORIGIN      
        1 tgttcctgta cgcgctgacg ctgtcgtcca ccgacatcgt gcccgtcgag gcggtgcgca
       61 tgtacaagga gcggctcagc gtcacgccgg tgggcaccgg tccgttcatg gcgaccgggc
      121 cgctcgatcg caagggcacg ctgcggctgg tgcgcaaccc cggttactac cggacgtatc
      181 cgtccaccgg cgcgccgggc gacgccggca aggggctgct caaggacgcc ggccgcaggc
      241 tcccgttcgt cgacgtcctg gagctgcccc tgatcgagga gccgcagccc gcggcgctga
      301 agttcctgcg cggcgagctc gaccgccgcg aactcgatcg cgcgaacttc accaggctgg
      361 tggcgcgcgc cccggacggc acgctgcgcc cggccgacgg ctacgcgggc aagttcacgc
      421 tggccagcga gccgggcggc aacgccatct acatcagcgt caacatgcgc gatccggtgc
      481 tcggcaggaa caaggcgctg cgccaggcga tcgccgcggc gatcgacccg cagtccgaca
      541 tcgacgtgct gtggaacggc cgcggcatcg cgatgcagac gctcgtgccg gtggagctcg
      601 cgggcagtgc gcacgacacc gccgcgccgc cgcacaagcg cgacgtcgcg ctcgccagga
      661 agctgctggc gcaggccggc ttccccgacg gcaagggcct gccgccgctg gcgatgcgct
      721 ttccgagcag cgacgcggcc acccgcaacg agttcgactt cttcaaggcg cagctcgcgg
      781 ccatcggcgt gcagctgacg ggcgacttcg acgacacgcc caccttcacc aaggcgctgg
      841 acgccggcaa cttccagctg gccagctaca gctactacgc cgactacccc gacgcggagg
      901 acttctacca gatgctgtac ggccgcaacg tctcgcccgg cccgaactac ggcgcgttcg
      961 cgaacgcggc ctacgacaag gcctacgagg cctcgcgcta catggtcaac ggtcccaagc
     1021 gcttcgagct gttcaggacg atggacgcca tcgtgcgcga cgaggtgccg ctgatccccc
     1081 tgcgcgagtc gctgcgcgtg gacacggtcc agccctgggt cgccaactac aagcgcaacg
     1141 tcttcaccac cgagatgcag ttcatgcgcg tcgacatggc ggccaagagg aaggggccgt
     1201 gaccatgcgc cttgtctcac cggccatcgc gcgggtgctg gcgtgcgcgg cgctcgcggg
     1261 cgcgctcacg gcacccgcgc acgcgcagga caagtcggtg ctcacggtgc cgcgcatctc
     1321 gcgcttcgag acgctcgacc cgcagcgcgg cttcggccag acggagacgc aggtcgtcga
     1381 ccaggtctac agccacctgg tcacctacgc ctacctcgag cggccctaca agctcgagcc
     1441 cgacctgctc gaggcgatgc ccacgctgtc gcccgaccag ctgacgctca ccttccacct
     1501 gcggccgggc gtgcgcttcg tcgacaacgc gtgctttccc ggcggcaagg ggcgcgagct
     1561 caccagcgac gacgtgctgt actcgctcaa gcgctacgcc gacggcaacg tcaacaccaa
     1621 gagctggttc gcgatgcagg gcgcggtcgt cggcctggac gactaccgcg cggccaccat
     1681 caaggccggc ccgtcggccg acctgacctc gcgcgacgtg gcgggcttgc gcaaggtcga
     1741 cgcccacacg ttcacgatcc gcctgacgca cccgaacgcg ctgttcctgt atgcgctgac
     1801 gatgtctccg acgtccatcg tgccgcgcga ggcggtgcag gcctacaagg atcgcttcgc
     1861 gctcaacccg gtgggcaccg ggccgttcat ggccacgcag caggtcgacc gcaagggcgt
     1921 catccggctg gtgcgcaacc cgaactacta ccgcacctat ccggcgacgg gcgagcccgg
     1981 cgacgcggcc gcgggccttc tcaaggacgc cggcaagcgc ctgccgctgg tcgacgaact
     2041 gcagatgccg ctgatcgagg agcagcagcc ggccgcgctc aagttcctgc acggcgacct
     2101 ggactgggag ccgatggacc gcgccagctt cgcaaggatg gtgcggcgca atcccgacgg
     2161 cacgttccgc ctggcgcccg agttcgcggg caagttcgcg atccacgccg tgcccagcct
     2221 cgagaccgac tacctgatgg tcaacatgcg cgatcccgtg ctgggtcgca acaagctgct
     2281 gcgccaggcg ctggcggccg cgatcgacgc gcgcgccatc gtcgacgtgc tgtggaacgg
     2341 ccgcggccgc ccgctcggca gcctcgtgcc ctgggacatc cccggcaacg agcgcgacac
     2401 cggcgccgtc gcgcgcccgc acgacctcgc gcaggccagg aagctgctgg cgcaggccgg
     2461 ctaccccgac ggccagggcc tgccgccgct gacgatcagc ttcttccaga cgcacgtggc
     2521 cgcacacaac gagttcgacc tgctcaaggc gcagctggcg gccgtcggcg tgcaggccaa
     2581 gggcgtcttc atggaccagc ccacgttcac gaaggccgcg gagaacggca actaccagct
     2641 ggcgtcgttc ggctgggagg ccgactatcc cgacgcggag gatttctacc agctgctgta
     2701 cggccccaac gcgccgccgg ccagcaactg gacgggctac gcgaacccgg cctacgacaa
     2761 ggcctacgag accgcgcgct tcatggccaa tggccccgaa cggctcgcgc agatgcgcat
     2821 catgaatgcg ctgatcgccg acgacgtgcc gctgatcgtg acgttcgacc cgctcaagtt
     2881 cggcatcacc caggcctggg tcggcaactt caagcgcaac ctgctgatcc aggagcacct
     2941 gttcctgagc gtggacatgg cacgcaagcg caagggcctg cagtgatggg cgagggggcc
     3001 cgctgatggg cgcgtacatc gtccgccggc tgctgcaggc cgtgccgacc gtgttcggcg
     3061 tcgcgctgct gaccttcctg ctgttcgaca ccttcggccc cgacccggtg cgcaccgcgc
     3121 tgggcaacca cgcgacggcc gaggcgatgg ccaacctgcg ccaccagtgg ggccttgaca
     3181 agccgctgcc gctgcagttc ctcgacttcc tgcgccagat cgccaccttc gactacggca
     3241 agtccttcgt caccggcgac gacctgggca cgcaattgaa ggacggcgcg ctcgtgagcc
     3301 tgtcggtgac gatgccgccg ttcctgttcg gcggcatggt caacatcgcc atcgcgctgt
     3361 tcatcgcacg ccatcgcgac ggcgtcgtcg acaaggccgc gcgcgcgctg ttcatcgcgg
     3421 cgatgagcgt gtcggcgctg gtgtgggtgc tggcgctgca gtacctgctg gcgttcaagc
     3481 tcgactggtt cccgatcaac ggctacgagg gcggctggtc ggcggtcaag tacctgggcc
     3541 tgccgtggct gatccagctg ctgctgctga tggggcccga catccgcctg taccgcgcgc
     3601 ttttcctggc cgacatgaac gccgactggg tgcgcacggc gcgtgccaag ggcgcgagcg
     3661 agagccgcgt gctgctgcgc cacgtgctgc ccaacgcgtg gatccccatc atcacgcaga
     3721 acgtcacgac gatcccgttc ctgatcctgg gctcgttcct gatggagcgc ttcttctcga
     3781 tcccgggcat cggcaacctg acgatcgacg cgctgtcgcg cggcgacctg ccgatcctga
     3841 aggccgtcac cgtgctgggc gcgctggcgc tggtcgtgtt caacctcgtc aacgacctgc
     3901 tgtacgcggt cgcggacccg cgggtgcggc tgcaatgagc gcgctgcccg atctcgtggc
     3961 cgccacgccc gcgccgcgcc cggccgcccg cgaggcgctg gccgcgctgt gggcgcgtcc
     4021 gcacatccgg ggctgcctga tcgtggtggc cctgtacgcg ctggtggctg cctgcggctg
     4081 gctgcacgtg ctgccggacg ccgaggccgc cgtcggcggt gcgcaggatc cgcccagctg
     4141 gcacctggcc acgctgctgg gcaccgacct gctgggccgc tcggtgctgt ggcgcgtgct
     4201 ggctggcgcg cagacggccg tcacgatcgg gctggccacg accgcgctgg cggtgccctt
     4261 cggcacggcg ctgggcctgg ccgcgggcta cttcggcggc tgggtcgacg cggtcgtcaa
     4321 ctgggtctac tcggtggtgg tgtcggtgcc cgacatcctg ctgatcacgg cgatctcgta
     4381 cgcgatgggc aagggcctga cgtcgatgtg cgtggcgatc gcggccacca gctgggtcgg
     4441 catcatgcgg ctggtgcgcg gcgaggtgct gcggcacaag ggcagcgact acgtgctggc
     4501 cgcgcgcagc gtgggcgcgg gcgcggcgcg cgtgatgttc tcggagatcc tgcccaacgt
     4561 cgtgcacgtg gcgatcgtca cgacgacgct ggtggtgctg gcggcggtca agggcgaggt
     4621 gatcctgacc tggctcgggc tgggcgtgca ggacggcgcc agctggggcc tgctgatcgc
     4681 gggcgcggcg caggatctca cgaacgacat ctggggcccg ctcgcgggca cggtgacggc
     4741 gatgttcctg ttgatctatt cgctgagcgc gatcggcgac gcgttgcgcg acgtattgga
     4801 tccgcgcgtg gcctgagtca ggccgcgcgg cgcagcgggg cgccgctgtc accgaagaac
     4861 acgttgaaga tctcgggctc gtcgatgtcg cgcaccgaca ggccttccag cggctcgcgg
     4921 tacaccacgg gcgcgttcat cgcatcgagg ctcaccggca tcgtctcgca ccaggtgggc
     4981 acgtcggcgg cgtcgtcctt gcgaacgacc aggtgctgca gcgacggctg ggcctcccac
     5041 gcgatcgaca tgtccggggt gcggtgggcc ttcagcggcg agttgttggc ggcgttcatg
     5101 atgagtccct ctcaggtttc caaaggtacg cctgaagggg ccgggccaat ggtcgagaag
     5161 aagggttttc ccggcccagt tgagggcgtg cggcatctgt ccacggcaac tgtggacaac
     5221 tctgtggggc ggccttggga agtgctcgcc aggccgcatg gatcctgggt gtcaagcgaa
     5281 ctgctgagga atggggcagc gcgacgcaaa cttttttcga ttgacatcgg acagggaaat
     5341 ccggcccgca gactatccac ggcaactgtg gacaactctg gggagtggtc ctgggaagtg
     5401 ttcgccagcc cgcatggata ctgggtgtca agcgaactgc tgcggaatcg ggcaggtgga
     5461 caaaactttt ttccgggccc tccgaagggg acgtttcggc cctttttcgt gcttggaacg
     5521 gcggcttgcc ggacgtccgt ccagggtcgg tcaggcgctc accggcacca gcatcggacg
     5581 cgcgatccgg acgtagtcgg ccgagtccag cacgaccgag cggtcgagcc ggccggcgtt
     5641 gaacgcgatc tcggcgaact gctcgaccag cgccgggtcg acgaccagtc gctgcgacgg
     5701 gccgtccacg aacggcggga tggcgccgat cgcgcaaccg gtggtggcca tcgcctcctc
     5761 ggggctggcc agcgtcgcct tcttcatgcc cagcgcctgc gcgacgcgct tgaagtccag
     5821 cttgcgatcg cccggcagca ccaccagcgc gtaggccgga tcgccgtcct tgctcctgca
     5881 cagcatcgcc ttggcgccct ggccgggcgc ggtgccgcgg atctgcgcga cgaggtcgga
     5941 gcggccttcg gccgggtgct cgatgacgcg aaagcgcgcg ccgccggcct cgagcaacgt
     6001 gacgagcgat tcgaactgca tgggcgggcc tcgggatccc cagggtcatg gcggcacgat
     6061 cggcggcgcc ggtcgcgcaa cgaaaacggc cggcacccgc ctcgcggcga atgccggccg
     6121 tgcgatggga cgagacgcgt cagcgcgcgg cgcggcggcg cgccatcacg cccagcgcgc
     6181 ccaggcccgc cagc
//
LOCUS       DATCRO010000387         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_510785, whole genome shotgun sequence.
ACCESSION   DATCRO010000387 DATCRO010000000
VERSION     DATCRO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510785"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIN75_10480"
     CDS             <1..322
                     /locus_tag="VIN75_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY8974633.1"
                     /translation="ARLATENLRRSGASNVSVVQADGSHGYASEGPFDAILLSGSVSK
                     VPEELLKQLKPGGRLVAIEGDEPVMAAVRYTANADGTFTRTSLFDLDAPRLVGFPEPA
                     RFHF"
     gene            548..1963
                     /locus_tag="VIN75_10485"
     CDS             548..1963
                     /locus_tag="VIN75_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8974634.1"
                     /translation="MSITRPGLASMSPAVRPIVGALLLAFGAAGAQAQSLTEVVQAAR
                     SVDATYLGARANADAAHYRTEQSRSTHLPTAGLAVQAQRQGQTSPTAVGVSAPTPTNP
                     NNVEEVATTEKQTSRSIGATFNASQTIYNRANNIAIDQAAKLEEVAQSQLAQADQDLI
                     VRVSQAYFDVLAAADALHSVEGNSKAIAEQLASAKRNFEVGNATITDTREAEARADLA
                     HSQQIAAENDLLVAKVKLDQLVGRNGVTPHPLVLPAVLPPVMPSQVSDWVALAEADSP
                     ALKQARLGLEAAELGTDKAKAGNYPTLGLSASYGRNRQRDSISPPGGSLTGNGNTSSI
                     ALTLNVPLFSGFLIQNQVREAAALQEKARTDAEGAHNQVVLGTRTAFVTAQTQAAQVK
                     ALEAAEASSKLALDATLLGYKVGVKVNLDVLNAQSQLYSTQKDAAGARYNYLMSQLKL
                     RQAAGSLTNNDLLPIDALLVK"
     gene            2140..>3929
                     /locus_tag="VIN75_10490"
     CDS             2140..>3929
                     /locus_tag="VIN75_10490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8974635.1"
                     /translation="MTAWDGSLETFRLLANSLQAMIAYYDSEELGYGCRFANKSYAAA
                     FGLTETSIIGKTFTEIIGEAAAREVKPSIDTVVNESRAVAYVRELAAPDGSKRWIEVN
                     VLPNLPREGRPVGAFVIVTDITGHRLATQALRESEERLAKFMQASAEGIAFHRDGIVT
                     DANPPLCEMLGYTLDEVRGRPALDFVASDHRPHVQAVLASGMDASYESAVIRKDGRRI
                     PVELIGRTLVYGGEKLRMSVVRDLRDRHAAQARIHHLAHHDALTGLPNRMSFMERLGQ
                     QIELARAAGKSLALLFVDLDHFKRVNDSLGHLIGDKLLQTVAARISASLRTNDVVARF
                     GGDEFIVLLDDAGRDDVAQVAQKLLRAIELPVEAEGRDLSVTPSVGVALFPHDGQTPT
                     ELIKNADTAMYIAKSRGRANCQFFDPAVASAAYDALVLESQLTQALQREEFVLHFQPQ
                     VRARDGALAGIEALIRWMHPERGMLRPDAFIALAEQQRVMLPISQWVLRSAARAALQW
                     RSQGLIDVPIAVNLSSMQFRANGFVDAVAQVLREEGVPGQWLELELTERMLMDDLGDV
                     KRTLQQLKALGMRVSVDDFGTGYSSLAHLK"
BASE COUNT          652 a         1440 c         1312 g          525 t
ORIGIN      
        1 ggccaggctg gccaccgaga acctgcgccg cagcggcgcg tccaacgtca gcgtggtgca
       61 ggccgacggc tcgcacggct acgcctcgga aggcccgttc gacgcgatcc tgctgtccgg
      121 ctcggtctcc aaggtgcccg aagaactgct caagcaactc aagccgggcg gccgcctcgt
      181 ggcgatcgag ggcgacgagc cggtgatggc ggcggtgcgc tacacggcca acgccgacgg
      241 cacgttcacc cgcaccagcc tgttcgacct cgacgccccg cgcctggtcg gcttcccgga
      301 acccgcgcgg ttccatttct gatcccatag tcctcgaaag agacagcgcg taccggaagg
      361 ccctggcggc cgtccggggg aacgctgcgc cccgtcgatc gatcgcggcc cgtccgcagt
      421 ctgtcggctg aaaacgtcgt cagggggtgc gtcggctgca ggtcgtcgca tcgagcgtgc
      481 atctcggccc gcaaggccgt acaaacctgt cccaacgcca aacggccaga acaaccaagg
      541 atcccgcatg tccattacgc gtcccggtct tgcctcgatg tcgccggccg tcaggccgat
      601 cgtcggagcc ctcctcctgg ccttcggcgc cgccggtgcc caggcccaga gcctgaccga
      661 agtcgtgcag gccgcgcgca gcgtcgacgc cacgtacctg ggcgcgcggg ccaatgccga
      721 tgcggcgcac taccgcacgg agcagtcgcg ctcgacgcac ctgccgaccg ccggactcgc
      781 ggtgcaggcg cagcggcagg gccagacatc gccgaccgcc gtgggcgtgt ccgccccgac
      841 gccgacgaac ccgaacaacg tggaggaagt ggccacgacg gagaagcaga cgtccaggag
      901 catcggcgcg accttcaacg cctcgcagac gatctacaac cgcgccaaca acatcgcgat
      961 cgaccaggcc gccaagctcg aggaagtggc ccagtcgcag ctcgcccagg ccgaccagga
     1021 cctgatcgtg cgcgtgtcgc aggcctactt cgacgtcctg gccgccgccg atgcgctgca
     1081 ctcggtcgaa ggcaacagca aggcgatcgc cgagcagctc gcgtcggcca agcgcaactt
     1141 cgaggtcggc aacgccacca tcaccgacac gcgcgaggcc gaggcgcgcg ccgacctggc
     1201 ccattcgcag cagatcgccg cggagaacga tctgctggtg gccaaggtca agctcgacca
     1261 gctcgtcggc cgcaacggcg tcacgccgca tccgctggtc ctgcctgcgg tgctgccgcc
     1321 ggtgatgccc agccaggtct cggactgggt cgcgttggcc gaagccgaca gcccggcgct
     1381 caagcaggcc cggctgggcc tcgaagcggc ggaactgggc acggacaagg ccaaggccgg
     1441 caactatccc acgctgggcc tgagcgccag ctacggccgc aaccgccagc gcgactccat
     1501 ctccccgccg ggcggctcgc tgacgggcaa cggcaacacc agcagcatcg cgttgacgct
     1561 caacgtgccg ctgttctccg gcttcctgat ccagaaccag gtgcgcgagg ccgccgcgct
     1621 gcaggagaag gcccgcaccg acgccgaggg cgctcacaac caggtcgtgc tcggcacccg
     1681 aaccgccttc gtcaccgcgc agacgcaggc cgcgcaggtc aaggcgctcg aggccgccga
     1741 ggcgtccagc aagctggcgc tcgacgccac gctgctggga tacaaggtgg gcgtgaaggt
     1801 caacctcgac gtgctcaacg cgcagtcgca gctgtactcc acgcagaagg acgccgcggg
     1861 cgcgcgctac aactacctga tgtcgcagct caagctgcgc caggccgcgg gcagcctgac
     1921 gaacaacgac ctgctcccca tcgacgccct gctcgtcaaa tgagccgcag ggcggcttgg
     1981 ccgaaggcgt aacccgccgt tcgcgcgcac gggcgccgcg cgccccgtcg cagcgatccc
     2041 cccatccggg cgattccccg ggaaaacatc accgcccatc tgggggtgac ggcgccgcca
     2101 cgcccgcgcc tatgatcctg catggccgat catgctggag tgactgcctg ggacggcagc
     2161 ctggagacgt tccgcctgct cgcgaacagc ttgcaggcga tgatcgccta ttacgattcc
     2221 gaagagcttg gctatggctg ccgcttcgcc aacaagagct atgcggcggc cttcggcctg
     2281 accgagacgt cgatcatcgg caagaccttc accgagatca tcggcgaagc cgccgcgcgc
     2341 gaggtcaagc cctcgatcga caccgtcgtc aacgagagcc gtgccgtcgc ctacgtgcgc
     2401 gaactggcgg cgccggacgg cagcaagcgc tggatcgagg tcaacgtgct gcccaacctg
     2461 ccgcgcgagg gccggccggt gggcgcgttc gtcatcgtca ccgacatcac cggccaccgc
     2521 ctggccacgc aggcgctgcg cgagtccgag gagcgcctgg ccaagttcat gcaggccagc
     2581 gccgagggca tcgcgttcca ccgcgacggc atcgtcaccg acgccaaccc gccgctgtgc
     2641 gagatgctgg gctacacgct ggacgaggtg cgcggccgcc ccgccctcga cttcgtggcg
     2701 tccgaccatc gtccccatgt gcaggcagtg ctggcgagcg ggatggacgc cagctacgag
     2761 agcgccgtca tccgcaagga cggccgccgc atcccagtgg agctcatcgg ccgcacgctg
     2821 gtctacggtg gcgagaagct gcgcatgtcc gtggtgcgcg acctgcgcga ccgccacgcg
     2881 gcgcaggcgc gcatccacca cctggcccac cacgacgcgc tcacaggcct gccgaaccgc
     2941 atgtcgttca tggagcggct gggccagcag atcgagctgg cgcgcgcggc gggcaagtcc
     3001 ctggcgctgc tgttcgtcga cctggaccac ttcaagcgcg tcaacgattc cctgggccac
     3061 ctgatcggcg acaagctgct gcagaccgtg gccgcgcgca tctcggccag cctgcgcacc
     3121 aacgacgtcg tcgcccgctt cggcggcgac gagttcatcg tgctgctgga cgacgcgggc
     3181 cgcgacgacg tggcgcaggt ggcgcagaag ctgctgcgcg ccatcgagct gccggtcgag
     3241 gccgaggggc gcgacctgtc ggtgacgccg tcggtgggcg tcgccctgtt cccgcacgac
     3301 ggccagacgc ccaccgagct gatcaagaac gcggacacgg cgatgtacat cgccaagtcg
     3361 cgcggccgcg ccaactgcca gttcttcgac ccggccgtcg ccagcgccgc ctacgacgcg
     3421 ctggtgctcg agagccagct cacgcaggcg ctgcagcgcg aggagttcgt gctgcacttc
     3481 cagccgcagg tgcgtgcccg cgacggcgcg ctcgccggca tcgaggcgct gatccgctgg
     3541 atgcatcccg aacgcggcat gctgcgcccc gacgcgttca tcgcgctggc ggagcagcag
     3601 cgcgtgatgc tgccgatcag ccagtgggtg ctgcgcagcg cggcgcgcgc ggcgctgcag
     3661 tggcgctcgc aggggctcat cgacgtgccc atcgccgtca acctctcgag catgcagttc
     3721 cgcgccaacg gcttcgtcga cgcggtggcg caggtgctgc gcgaggaagg cgtgccgggc
     3781 cagtggctgg agctcgagct caccgagcgc atgctcatgg acgacctggg cgacgtcaag
     3841 cgcacgctgc agcagctcaa ggcgctcggc atgcgcgtct cggtggacga cttcggcacc
     3901 ggctactcgt cgctcgcgca cctcaagga
//
LOCUS       DATCRO010000388         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_814233, whole genome shotgun sequence.
ACCESSION   DATCRO010000388 DATCRO010000000
VERSION     DATCRO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814233"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VIN75_10495"
     CDS             <1..455
                     /locus_tag="VIN75_10495"
                     /inference="COORDINATES: protein motif:HMM:NF042598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6765 family protein"
                     /protein_id="HEY8974636.1"
                     /translation="AVARDRLATLITGATDDDGDARLRQWLAALAADHFGFGPSTPTY
                     VGKGKGSWKHVALGEFYLSWLQSAQRELEAIPLEKRSLLQRIGAKLLEAGAEHTEALA
                     GHFNIESVTYPFNPAFLSSHYKRFHDAAEEQRFAVFKKILPRFGIYEA"
     gene            complement(695..1354)
                     /locus_tag="VIN75_10500"
     CDS             complement(695..1354)
                     /locus_tag="VIN75_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974637.1"
                     /translation="MATASSGPDVTPASAIRLLLVDDHPLVRDGLRARLEAVPGFEVV
                     GEASNAQEAHAQLGVLQPTLVLMDVGMKGVNGIDLTSALLEREPALLVLMLSMYDNPE
                     YVQRAMQAGARGYVLKDSPASEIIAAIEAVAGGGTFLSPAVSRRLFRNQTPRPILSSR
                     ECEILAALARGLSSKHIAREMDVSVRTVEAHRQNIKRKLSLEGQAELIKYAVEHAGKL
                     G"
     gene            complement(1425..2720)
                     /locus_tag="VIN75_10505"
     CDS             complement(1425..2720)
                     /locus_tag="VIN75_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974638.1"
                     /translation="MTLTGAAKGPSDQKTLNEEIIEGLAEVARRNPQHAVELFGVPLG
                     VAMRLAAMPDAQLDDLATTPVALWRPALTERLVRRLPERRPMLTPDHEVYRPLVKRIN
                     RLALATFVRYADDPASAALVCDITDREVIRALQELPAFAFLEWGGNPGALLAVPRLSD
                     PLIDRLLRPDDDGVEPNLRGLFALTQCCEQEFALIRHAVQQESAAYRVAERTPVNKRS
                     GRPPATFLMPGTSSLILAMLRHQMRPTEVEDRMLATSDVRAAQLRGIAETLNPKVKRE
                     RGAPRDRTRDHRDLWGSAHRRLSATAIFCLQRRLVDAGMEAFEAMVWAYDYYACTYDA
                     DCGVSLSRMLKDVFTPMREGADTHLGHCAKCGVVHLAHEEKVGIIECPVCVLARSGKF
                     GQRRVRAGGDKPEMPALRDYRGWPQAAARTSSGLGATRG"
     gene            2924..3799
                     /locus_tag="VIN75_10510"
     CDS             2924..3799
                     /locus_tag="VIN75_10510"
                     /inference="COORDINATES: protein motif:HMM:NF020251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8974639.1"
                     /translation="MEDLLQARPGTDNDAPTEPALSVNVRRFLLSHGVKEHNHASEIA
                     RIINVDRSQSYRRLKGDVTWSHTDLRAIAHHFGAPDELLLPRDDLARFTHPGSDGVPA
                     LLRVPGLPPTGLLVPGPELAPGESSDLVAIRAGASWEVHVNGDEPIGARRHAIEALTI
                     RSHPHLQVALLEDDLRAADALVEAFRPLGIVATPYADSDALRDAIAHRRFDAYVLDWL
                     LAEGTAASIVEEIRGLRAHVPILVVTGALMADSEMEKSLLELSAQWNFSTLDKPVRPM
                     NLANALKQLVAAARA"
BASE COUNT          567 a         1347 c         1338 g          577 t
ORIGIN      
        1 acgccgtcgc gcgcgaccgg ctcgcgacgc tcatcaccgg agccaccgac gacgacggcg
       61 acgcgcgcct gcgccaatgg ctggccgcgc tggcggccga ccacttcggc ttcggaccat
      121 cgacgccgac ctacgtcggc aagggcaagg gctcgtggaa gcacgtcgcg ctcggcgagt
      181 tctacctgag ctggctgcag tcggcgcaac gcgagctgga ggcgatcccg ctcgagaagc
      241 gatcgctgct gcagcgcatc ggcgccaagc tgctcgaggc cggggccgag catacggagg
      301 cgctggccgg gcacttcaac atcgagtccg tcacctaccc gttcaacccg gcgttcctct
      361 cgagccacta caagcgcttc cacgacgccg ccgaggaaca gcgcttcgcc gtgttcaaga
      421 agatcctgcc gcgattcggg atctatgagg cctgaagggg gcgccgcgtt gcttgtagaa
      481 agcgcttctg gacgcgtgga tcctgcgact gcgcgcagga tgacactggg tgggcgcgat
      541 gcggcgacgg tgcggcacgc ggagcgccaa gccgtcatcc tgcgcgtggc gaagcgcagt
      601 cgcaggatcc cgccggcgag gcggcttcgt ggccggctgg catcgtgcga ctgcgcgcgc
      661 gaaatgacac gggaggaccg gcgtgcggcg cgccctaccc cagcttgccc gcgtgttcga
      721 ccgcgtactt gatcagctcc gcctgccctt cgagcgacag cttgcgcttg atgttctggc
      781 ggtgcgcctc caccgtgcgc acgctcacat ccatctcgcg ggcaatgtgc ttgctggaca
      841 ggccgcgggc cagcgccgcc aagatctcgc attcgcgcga cgacaggatg ggacgcggcg
      901 tctggttgcg gaacaggcgc ctggacaccg ccgggctgag gaaggtgccg ccgccggcga
      961 cagcctcgat ggccgcgatg atctcgctgg ccggcgagtc cttgagcacg tagccgcgcg
     1021 cgccggcctg catggcgcgc tgaacgtact cggggttgtc gtacatggac agcatcagca
     1081 ccagcaacgc gggctcgcgc tccagcaggg cggacgtgag gtcgatgccg ttgacgccct
     1141 tcatgcccac gtccatcagc acgagcgtgg gctgcagcac gccgagctgc gcatgggcct
     1201 cttgcgcatt ggacgcctcg ccgaccacct cgaacccggg cacggcttcg agccgcgcgc
     1261 gcaggccatc gcgcaccagc ggatggtcgt cgaccagcag cagtcggatc gcggacgcgg
     1321 gtgtcacgtc ggggccggaa gaagcggttg ccatgccacg attagaccgc agcgtcgccg
     1381 ctcgccgtca acagcggcct cgcgcggccg gccgcgctgc gtccctaccc gcgcgtcgcc
     1441 cccaggccgg agctcgtccg ggccgccgcc tgcggccagc cgcggtagtc gcgcagggcc
     1501 ggcatctcgg gcttgtcgcc gcccgcgcgg acgcggcgct ggccgaactt gcccgagcgc
     1561 gccagcacgc agacgggaca ctcgatgatg cccaccttct cttcgtgcgc cagatgcacc
     1621 acgccgcact tggcgcagtg gcccaggtgc gtgtcggcgc cctcgcgcat cggcgtgaag
     1681 acatccttga gcatgcggct gagcgacacg ccgcagtcgg cgtcgtaggt gcacgcgtag
     1741 tagtcgtagg cccagaccat ggcctcgaag gcctccatgc cggcgtcgac caggcggcgc
     1801 tgcaggcaga agatggccgt ggcgctgagg cggcgatggg cgctgcccca caggtcgcgg
     1861 tggtcgcgcg tgcggtcgcg cggcgcgccg cgctcgcgct tgaccttggg gttgagcgtc
     1921 tcggcgatgc cgcgcagctg cgcggcgcgc acgtcgctgg tggccagcat gcggtcctcc
     1981 acttcggtgg gccgcatctg gtggcgcagc atcgccagga tcaggctgga cgtgcccggc
     2041 atcaggaagg tggccggcgg ccggcccgag cgcttgttga ccggcgtgcg ttcggccacg
     2101 cgataggcgg cgctttcctg ctgcacggca tggcggatga gcgcgaactc ctgctcgcag
     2161 cactgcgtga gcgcgaacag gccgcgcagg ttgggctcga cgccgtcgtc gtcgggccgc
     2221 agcaggcggt cgatcagcgg gtcggacagg cgcggcaccg ccagcagcgc ccccgggttg
     2281 ccgccccatt cgaggaaggc gaaggcgggc agctcctgca gcgcgcggat cacctcgcgg
     2341 tcggtgatgt cgcacacgag cgcggcgctg gcggggtcgt cggcgtagcg cacgaaggtg
     2401 gccagcgcga gccggttgat gcgcttgacc agcgggcggt agacctcgtg gtcgggcgtg
     2461 agcatgggcc ggcgctcggg cagccggcgg acgaggcgct cggtgagcgc ggggcgccac
     2521 agcgcgacgg gcgtggtcgc caggtcgtcg agctgcgcat cgggcatcgc ggccaggcgc
     2581 atcgccacgc ccagcggcac gccgaacagc tcgaccgcgt gctgagggtt gcggcgcgcg
     2641 acttccgcca ggccttcgat gatttcctcg ttgagcgtct tctggtcgct cgggcccttg
     2701 gccgctccgg tcaacgtcat tctttcccct tgtgttccag gtgagctcgc caaccccatc
     2761 gtgttgtgat ttgccacaaa caattcagcg aatgatgttc aaaatgttgg cgtgatgtga
     2821 ctgtgacaca caagtttcag cccatcaatc cgaagtttgt gggcattttg ctgtgatccg
     2881 gtctaaactg tccgttcccc ctagcttggc gacatggagt ccgatggaag acctgctgca
     2941 agcccgcccc ggcaccgaca acgacgcgcc caccgagccc gcgctcagcg tcaacgtgcg
     3001 ccgcttcctg ctgtcgcacg gggtcaagga acacaaccac gcgtccgaga tcgcgcgcat
     3061 catcaacgtc gaccgctcgc agtcgtaccg ccggctcaag ggcgacgtga cgtggagcca
     3121 caccgatctg cgggccatcg cccatcattt cggcgcgccc gacgaactgc tgctgccgcg
     3181 cgacgacctc gcccgcttca cgcatcccgg cagcgatggc gtgccggccc tgcttcgggt
     3241 gcccggcctg ccgcccaccg ggctgctggt gcccggcccg gaactggcgc cgggcgaaag
     3301 cagcgacctc gtcgcgatcc gcgcgggcgc cagctgggaa gtgcacgtca atggcgacga
     3361 gcccatcggc gcacgccgcc atgccatcga ggcgctgacg atccgcagcc atccgcacct
     3421 gcaggtggcg ctgctggagg acgacctgcg cgccgccgac gcgctggtcg aggccttccg
     3481 cccgctgggc atcgtggcca cgccctatgc cgacagcgac gcgctgcgcg acgccatcgc
     3541 gcaccgccgc ttcgacgcgt acgtcctgga ctggctgctg gccgagggca ccgccgcgag
     3601 catcgtcgag gagatccgcg gcctgcgcgc gcacgtgccc atcctcgtgg tgacgggcgc
     3661 gctgatggcc gactccgaga tggagaagag cctgctcgag ctgtcggccc aatggaactt
     3721 ctccacgctc gacaagccgg tgcgcccgat gaacctcgcc aacgcgctca agcagctggt
     3781 ggccgccgca cgggcctgag gtcttcgtcg cgcccgcaca atgcgtgca
//
LOCUS       DATCRO010000389         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_330488, whole genome shotgun sequence.
ACCESSION   DATCRO010000389 DATCRO010000000
VERSION     DATCRO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330488"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VIN75_10515"
     CDS             complement(<1..306)
                     /locus_tag="VIN75_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974640.1"
                     /translation="MAASLPPKTAAFRAEYRAEVLPARYSGAAHVALVLVAAGGGVAA
                     AIAFAAAHGARWADAWIAPLTFVVANVVEYAAHRGPMHHRTAALAALHTRHSRRHHRF
                     "
     gene            complement(452..1009)
                     /locus_tag="VIN75_10520"
     CDS             complement(452..1009)
                     /locus_tag="VIN75_10520"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEY8974641.1"
                     /translation="MTARTFGLFAAAALVAGCATHPAPPPAIAAPADVPAALQPAAGQ
                     EPFLELHASGVQVYECAARADVPAAWAWQFRSPEASLADAQGHVVGRHFAGPSWTSND
                     GATVVGQVAASAPSPEAGSIPWLLLTVKSRDGRGVLAAATSVQRLDTEGGVAPRSACG
                     EANAHQLERVGYVATYVFWRARPAG"
     gene            complement(1006..1542)
                     /gene="sodC"
                     /locus_tag="VIN75_10525"
     CDS             complement(1006..1542)
                     /gene="sodC"
                     /locus_tag="VIN75_10525"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HEY8974642.1"
                     /translation="MKIAFTLALAAALATPALSHAAETTVTLNLAQADGTGPAVGTVR
                     LVETRYGLALYPSLTGLPPGLHGFHVHENASCAASTKDGVTTPAGAAGGHLDPLGSKH
                     HGEPWGDGHLGDLPPLYVAADGSAGNPVLAPRLKLADVAHHALMVHAGGDNHSDHPAA
                     LGGGGARVACGVIEGPAQ"
     gene            complement(1576..2076)
                     /locus_tag="VIN75_10530"
     CDS             complement(1576..2076)
                     /locus_tag="VIN75_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974643.1"
                     /translation="MQAITRTLTLAATATLLALGAALAHASDSQPEAPPARASADKLA
                     QARALIAARNWSGALDELRRVNDTGSADWNNLMGFTLRKGKTPDLAGSEKYYDTALRI
                     DPHHRNTLEYSGELYLMKGDLPKAQARLAALQTECGGSCEQLTQLKTAIADYQAHGNK
                     FVSTGW"
     gene            complement(2183..2929)
                     /locus_tag="VIN75_10535"
     CDS             complement(2183..2929)
                     /locus_tag="VIN75_10535"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8974644.1"
                     /translation="MNYLRPELLDALARAHALGTLSPGAARRFTRVVAGSAVAAKAVA
                     DWQGVLQTLEQGAPPAPQPRPRVWEGVQQRLFERHERARAPAGGTRAAGRGLAGRLGW
                     PVGLVAGALLSWSLLVLRPQAFDMEAASGAAPASYVGVLQDPQGHPLLATTARRHGHV
                     LTVRLLRPVAIEPGHALTVWAWSDADPTPRRVGSWTTAGSTSTIALPGPAEALLGKMT
                     HLGLSDEADGVPPASPVRPFIAEGACAKVW"
     gene            complement(2926..3522)
                     /locus_tag="VIN75_10540"
     CDS             complement(2926..3522)
                     /locus_tag="VIN75_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8974645.1"
                     /translation="MAQRNDPPDNDDLARWLSRIALGDRQAFEQLYRATSGYLMSIAW
                     RVLQHRDLAEEVLQDAFVKVWHAAAMYDARLGLPMTWLINIVRNRAIDLRRSRSQALV
                     NSHGDDDAERIDATAAPGAGPEQWLDTAIQRIGVQECMGQLTPAQRQALALVYYQGLT
                     HVEVAQALEAPLGTAKAWVRRGLDYLRACLQGRGMAAS"
     gene            complement(3711..4490)
                     /locus_tag="VIN75_10545"
     CDS             complement(3711..4490)
                     /locus_tag="VIN75_10545"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY8974646.1"
                     /translation="MCQLLGMNANTPTDAMFSFTGLATRADEHKDGFGIAFFEDRGLR
                     LFVDQHSARNSPVAELVKGYPIRSDNVIAHIRKATQGRIALENTHPFMRELWGRYWVF
                     AHNGDLKDFRPRLHGTFRPVGDTDSEHVFCWLMQELSKAHAGVPAIDELSITLSELLP
                     QLHRHGTFNMLLSNGQALWAHCSTHLHYVVREHPFGAPVLADKDLMVDFAPHTGPGDR
                     VAIVATEPLTRNETWKPMAPGQLMTFTGGAVATRQCAALAR"
     gene            complement(4562..>5705)
                     /locus_tag="VIN75_10550"
     CDS             complement(4562..>5705)
                     /locus_tag="VIN75_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranyl reductase"
                     /protein_id="HEY8974647.1"
                     /translation="VDQHDFPRDKTCGDGLIPDAHHALARLGVHDEVMALARRARHVG
                     CVGPRGGRVDVPGELAVLPRRILDDIVCRAAVRAGARLRTPWRFEATLAEGDTIVGAR
                     LKQGETLRELRARWVVLATGAVPQALQAADMCKRRTPSGIALRGYVKNDAMVGRIKAL
                     EVVWHKSLSPGYGWIFPAPGGRFNIGVGIAHSHPARDGEPARMKDVNLRDVFKAFCDH
                     YEPARELMRTGALEGDLKGAPLRCSLAGARLSKPGLLVTGEAAGSTYSFTGEGIGKAY
                     ETGLLAAEALVDWPGFAESGVPAGTFPETRTRYEAALAALQPRFDLYERANRVNAHPW
                     LADLLIWRAQKSERLLRRMAGVLEETSNPGNLVSARGILQVLFPGG"
BASE COUNT          732 a         1989 c         2199 g          785 t
ORIGIN      
        1 gaaacggtgg tggcggcgcg aatggcgcgt gtgcagcgcc gcgagcgcgg cggtgcggtg
       61 gtgcatcggc ccgcggtgcg cggcgtactc gacgacgttg gcgacgacga aggtcagcgg
      121 cgcgatccag gcgtcggccc agcgcgcgcc gtgcgccgcg gcgaacgcga tcgcggcggc
      181 gacgcccccg ccggcggcca ccagaacgag cgcgacgtgc gcggcgcccg agtagcgcgc
      241 gggcagcacc tcggcgcggt actcggcgcg gaacgcggcg gtcttgggag gaagggacgc
      301 ggccatgggg gcgcatcttt cccgcgcaga tcacgccacg tcttgtcctg atgcgacacg
      361 gcgcccgcgg aaagcgccat ctcctggaaa gtcctgatcc tcgtttacca ggagattcca
      421 ggcccgccgc gacgccgggg cgacccgcgc gtcagcccgc gggccgagcg cgccagaaca
      481 cgtacgtggc gacgtatccg acgcgctcga gctggtgcgc gttggcctcg ccgcacgccg
      541 agcgcggcgc gacgccgccc tcggtgtcca ggcgctggac gctggtggcg gcggccagca
      601 cgccgcggcc gtcgcgcgac ttgacggtca gcagcagcca cgggatcgac ccggcctcgg
      661 gcgacggcgc gctggccgcg acctggccca ccaccgtggc gccatcgttc gacgtccagg
      721 acggcccggc gaaatggcgg ccgacgacgt ggccctgcgc gtcggccagg ctcgcctcgg
      781 gactgcggaa ctgccaggcc caggcggcgg ggacgtcggc gcgcgccgcg cattcgtaga
      841 cctgcacgcc gctggcatgc agctcgagga acggctcctg gccggcggcc ggttgcagcg
      901 ccgcgggcac gtcggccggc gcggcgatgg cgggcggggg cgccggatgc gtcgcgcagc
      961 cggccaccag cgcggcggcc gcgaacaggc cgaaggtgcg cgccgtcatt gcgccggccc
     1021 ctcgatgacg ccgcaggcca cccgcgcgcc gccgccgccg agcgcggcgg gatggtcgga
     1081 gtggttgtcg ccgccggcat gcaccatcag cgcgtggtgc gcgacatcgg cgagcttcag
     1141 gcgcggcgcg agcaccgggt tgccggcgct gccgtcggcc gccacgtaca gcggcggcag
     1201 gtcgccgagg tggccgtcgc cccagggctc gccgtgatgc ttgctgccca gcggatccag
     1261 gtggccgccg gcggcgccgg cgggcgtggt gacgccgtcc ttggtcgagg ccgcgcagga
     1321 cgcgttctcg tgcacgtgga agccgtgcag gcccggcggc aggccggtga gcgacggata
     1381 cagggcgagg ccgtagcgcg tctcgacgag gcgcaccgtg cccacggcgg ggccggtgcc
     1441 gtcggcctgc gcgaggttga gcgtgacggt ggtctcggcg gcgtgcgaga gggcgggcgt
     1501 ggccagggcg gcggccagcg cgagggtgaa tgcgatcttc atgggaagtt cctgggcaag
     1561 tggaggatgg cgggcttacc agccggtgga cacgaacttg ttgccgtgcg cctggtagtc
     1621 ggcgatcgcc gtcttcagct gcgtgagctg ctcgcacgag ccgccgcatt cggtctgcag
     1681 cgcggccagg cgcgcctggg ccttgggcag gtcgcccttc atcaggtaga gctcgccgga
     1741 atattccagc gtgttgcggt ggtgcgggtc gatgcgcaac gccgtgtcgt agtacttctc
     1801 ggagccggcg agatcgggcg tcttgccctt gcgcagcgtg aagcccatca ggttgttcca
     1861 gtcggcgctg ccggtgtcgt tgacgcggcg cagctcgtcg agcgcgccgc tccagttgcg
     1921 cgccgcgatc aacgcgcggg cctgggcgag cttgtcggcg gacgcgcgcg cgggcggggc
     1981 ttcgggctgg ctgtcggacg cgtgcgcgag cgcggcgccc agggccagga gggtggccgt
     2041 ggcggccagc gtgagggtgc gggtgatggc ttgcatggtg ctctccattt gatgttcgac
     2101 gggacgtccg aggaatccca cgcacttacg gtgccgatcg ccggacggat cacggtcggc
     2161 aaaaaaaatt cgcgcaaagc tgtcaccaga ctttcgcgca ggccccttcg gcgatgaacg
     2221 ggcgcaccgg ggacgccggg gggacgccgt cggcttcatc gctcaggccc aggtgcgtca
     2281 tcttgcccag cagcgcttcg gccgggccgg gcagcgcgat ggtggaggtc gatccggcgg
     2341 tcgtccagct gccgacgcgg cgcggcgtcg ggtcggcgtc gctccacgcc cagacggtca
     2401 acgcgtggcc gggttcgatc gcgacgggcc gcagcagccg cacggtgagc acgtggccat
     2461 ggcgccgcgc ggtggtggcc aggagcgggt ggccctgtgg atcctgcagc acgccgacat
     2521 agctggccgg tgccgcgccg gaggcggctt ccatgtcgaa ggcctgcggg cgcagcacga
     2581 gcagcgacca ggagagcagc gcgccggcca cgagtcccac cggccagccc agccggcccg
     2641 cgagtccgcg gcctgcggcg cgcgtgccgc cggccggcgc gcgggcgcgc tcgtggcgtt
     2701 cgaacaggcg ctgctgcacg ccctcccaca cgcgcggccg cggctggggc gccggcggcg
     2761 cgccctgttc cagggtctgc agcacgccct gccagtcggc caccgccttg gccgcgaccg
     2821 cggagcccgc gacgacgcgc gtgaagcgcc gcgccgcgcc gggcgacagc gtgcccagcg
     2881 cgtgggcgcg ggcgagggcg tccaggagtt cgggtcgcag gtagttcatg acgcggccat
     2941 cccgcggccc tgcaggcacg ccctcaggta gtcgagcccg cgccgcaccc aggccttggc
     3001 ggtgcccagc ggcgcctcga gcgcctgtgc gacctcgaca tgcgtcagcc cctggtagta
     3061 cacgagggcc agcgcctggc gctgcgccgg cgtcagctgg cccatgcatt cctgcacgcc
     3121 gatgcgctgg atggccgtgt ccagccactg ctcggggccg gcgccgggcg cggccgtcgc
     3181 gtcgatccgc tcggcgtcgt cgtcgccgtg ggaattgacc agcgcctgcg aacgcgagcg
     3241 gcgcaggtcg atggcgcggt tgcgcacgat gttgatcagc cacgtcatcg gcaaccccag
     3301 gcgcgcgtcg tacatcgcgg ccgcatgcca caccttgacg aacgcgtcct gcagcacctc
     3361 ctcggccagg tcgcggtgct gcagcacgcg ccacgcgatg gacatcaggt agccggacgt
     3421 cgcgcgatac agctgctcga aggcctgccg gtcgccgagg gcgatgcgcg acagccagcg
     3481 cgccaggtcg tcgttgtcgg gggggtcatt gcgttgtgcc atggtgcaag cgtgatccgg
     3541 cggcgccgtc ggatgggcgg cggcgcgatc ttatgtccgt cgtgcgagac ctgccggcgc
     3601 cggggacgca ggcacttacg gcgcggccgc gcagacggac gcacccgtca cgatgacgtc
     3661 tcgtcgcgac ctgggtggcc cgtggcccgc gatcccgagt cggcgccggc ctatcgggcc
     3721 agggccgcgc attggcgggt cgcgacggcg ccgcccgtga acgtcatcag ctggcctggc
     3781 gccatcggtt tccaggtctc gttgcgggtc agcggctcgg tggcgacgat ggccacgcgg
     3841 tcgcccgggc cggtgtgggg cgcgaaatcc accatcaggt ccttgtcggc gagcacgggc
     3901 gcgccgaacg ggtgctcgcg cacgacgtag tgcagatgcg tcgagcaatg cgcccacagc
     3961 gcctggccgt tggacagcag catgttgaag gtgccgtgcc ggtgcagctg gggcaacagc
     4021 tcggacagcg tgatcgacag ctcgtcgatc gccggcacgc ccgcatgcgc cttggacagc
     4081 tcctgcatca gccagcagaa cacgtgctcg ctgtcggtgt cgccgacggg gcggaaggtg
     4141 ccgtgcaggc gcggccggaa gtccttcagg tcgccgttgt gggcgaacac ccagtagcgg
     4201 ccccacagct cgcgcatgaa cgggtgcgtg ttctccagcg cgatgcgccc ttgggtcgcc
     4261 ttgcggatgt gggcgatgac gttgtcgctg cggatcgggt agcccttgac cagctcggcc
     4321 accggcgagt tgcgggcgct gtgctggtcg acgaacagcc gcagcccgcg atcttcgaag
     4381 aaggcgatgc cgaagccgtc cttgtgctcg tccgcgcgcg tggccagccc ggtgaagctg
     4441 aacatcgcgt cggtcggcgt gttggcgttc atgccgagca gttgacacat ggcccaagca
     4501 tacgccgcgc cgtcatgcga atggcgcggg tcgttcctcg atgcgtgtcg accggccggc
     4561 ctcagccgcc gggaaagagc acctgcagga tgccgcgcgc gctgaccagg ttgcccgggt
     4621 tgctggtctc ctcgagcacg ccggccatgc ggcgcagcag gcgctcgctc ttctgcgccc
     4681 gccagatcag caggtcggcg agccacgggt gggcgttgac gcggttggcg cgctcgtaca
     4741 ggtcgaagcg cggctgcagc gcggcgagcg cggcctcgta gcgcgtacgc gtctcgggaa
     4801 aggtgccggc cggcacgccg gactccgcga agccaggcca gtcgaccagc gcctcggcgg
     4861 ccagcaggcc cgtctcgtag gccttgccga tgccctcgcc cgtgaaggag taggtgctgc
     4921 ccgccgcctc gccggtgacg agcaggcccg gcttgctcag gcgcgcgccg gccagcgagc
     4981 agcgcagcgg ggcgcccttc aggtcgccct ccagcgcgcc ggtgcgcatc agctcgcgcg
     5041 ccggctcgta gtggtcgcag aaggccttga agacgtcgcg caggttgacg tccttcatcc
     5101 tggccggctc gccgtcgcgc gccggatggc tgtgggcgat gcccacgccg atgttgaagc
     5161 gtccgccggg ggccgggaag atccagccgt agccggggct caggctcttg tgccacacga
     5221 cctcgagcgc cttgatgcgg ccgaccatcg cgtcgttctt cacgtagccg cgcagcgcga
     5281 tgccgctggg ggtgcggcgc ttgcacatgt cggcggcctg cagggcctgc ggcacggcgc
     5341 cggtagccag caccacccac cgggcgcgca gttcgcgcag cgtctcgccc tgcttcaggc
     5401 gcgcgccgac gatggtgtcg ccctcggcca gcgtggcctc gaagcgccac ggcgtgcgca
     5461 gcctggcgcc ggcgcgcacg gcggcgcggc agacgatgtc gtcgaggatg cgccgcggca
     5521 gcaccgcgag ctcgccgggg acgtcgacgc gccccccgcg cggaccgacg cagcccacgt
     5581 gccgggcgcg ccgggcgagc gccatcacct cgtcgtgcac gcccaggcgc gcgagcgcgt
     5641 ggtgcgcgtc ggggatcagg ccgtcgccgc aggtcttgtc gcggggaaag tcgtgctggt
     5701 cgacg
//
LOCUS       DATCRO010000390         5452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_150623, whole genome shotgun sequence.
ACCESSION   DATCRO010000390 DATCRO010000000
VERSION     DATCRO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150623"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VIN75_10555"
     CDS             <1..938
                     /locus_tag="VIN75_10555"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520651.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8974648.1"
                     /translation="VFMLSPFGRMSPFQQAQMFSLQDANIHNLSVEGVFDDCQDIVKA
                     VSNDLAFKRKWRIGTVNSINWARLLAQVVYYFAGYFQATTSNDQKVAFTVPSGNFGNV
                     CAGHVARMMGLPIARLVVATNENDVLDEFFRTGTYRVRGSAETHETSSPSMDISKASN
                     FERFVFDLLGRDGGRTRDLFGAGLQRDGAFTLSAADKARIAEFGFVSGRSTHADRLAT
                     IRDTDARFGVVIDTHTADGLKVAREHLLPGTTMIVLETALPIKFAETIEEALGRKPAL
                     PAGLADLESRPRRFTKLPADVDAVKAFIEKNAPAQ"
     gene            1148..2416
                     /gene="glp"
                     /locus_tag="VIN75_10560"
     CDS             1148..2416
                     /gene="glp"
                     /locus_tag="VIN75_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8974649.1"
                     /translation="MNAPDAKPAGRGPLISADEALARLLSAVVPTGRSETVPTPEAWG
                     RVLSRDIVSSVHVPPEDNSAMDGYALRVADLAEGAGSVLRVSQRIVAGRPGEPLQPGT
                     AARIFTGAQVPAGADAVVMQEHCEAVPGPGLGTVRVNEVPAPGTAIRRQGEDVRRGHV
                     VLRAGHRMDAAAIGLAATVGMATLEVAARPRVALFSTGDELVMPGEPLPPGAIYNSNR
                     FMLGALLRGQGCVVTDLGNVPDKLDATRAMLREAAGGHDLILSSGGVSVGEEDHLKPA
                     LAAEGRLDLWAIAIKPGKPLAFGAVRNGAAQGGETWFIGLPGNPVSSHVTFTLFVRPV
                     LARLQGAEAAWPAGVPMRADFAWPRPDKRREFLRVRRNASGGLDLYANQGSGVLTSLA
                     WGDGLADLAPGQAVQPGDLVRFLSLAELAP"
     gene            2413..2670
                     /locus_tag="VIN75_10565"
     CDS             2413..2670
                     /locus_tag="VIN75_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8974650.1"
                     /translation="MKLTLKYFASLRETLGAGGPLEWTPEAEPTAAALRAWLRTQSPA
                     HAQALAPGRAVRVAVDQALADESATLRDGAEVAFFPPVTGG"
     gene            2672..3163
                     /gene="moaE"
                     /locus_tag="VIN75_10570"
     CDS             2672..3163
                     /gene="moaE"
                     /locus_tag="VIN75_10570"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEY8974651.1"
                     /translation="MASSRVRIQEADFDVGAELAALQGGRTDVGAVASFTGLVRGHTR
                     EDDISALTLEHYAGMTERAIEAMIAEAQARFGLQAALVIHRVGRLLPGERIVLVAVAA
                     PHRRAAFQGCEFLMDYLKTHAPFWKKETTPDGERWVDARVADDEALRRWGLQSANASS
                     GTP"
     gene            3334..3870
                     /locus_tag="VIN75_10575"
     CDS             3334..3870
                     /locus_tag="VIN75_10575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8974652.1"
                     /translation="MSAPTDPTSHPAVPEIAASELVPDELRRALGRFVTGVTIVTCRD
                     EHGALVGLTANSFNALSLDPPLVLWSLRESSSTISAFTGASHFAINVLAADQVELSRR
                     FARPSSAKFDAGEWTDGQGGAPLLAGCVAVFECRRRSHHPAGDHVLFIGEVERIGGSA
                     STPLVYHAGHYRTLGDLE"
     gene            4061..>5452
                     /locus_tag="VIN75_10580"
     CDS             4061..>5452
                     /locus_tag="VIN75_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY8974653.1"
                     /translation="MRLDKLTTAFQQALADGQSAAVARDNPYVEPAHVLAAMLAQPDG
                     PKSLLDRAGANTAALRTAMDTLVAGLPQVQGGGQVSGSRDLVHLLQQAEKEAGKRGDQ
                     FVASEMFLLALADAKTDIGGIVRGHGLTRKALEAAIDAVRGGATVDSADAEGQREALK
                     KYTLDLTERARSGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLA
                     QRIVAGEVPETLKNKRVLVLDMAGLLAGAKFRGEFEERLKAVLKEVALDEGRIILFID
                     ELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRKYVEKDAALERRFQKV
                     LVDEPSVEATIAILRGLQEKYEVHHGVEITDPAIVAAAELSDRYITDRFLPDKAIDLI
                     DEAAAKVKIEIDSKPEVMDRLDRRIIQLKIEREAVRRETDEASQKRLGLIEEEIARLG
                     REYADLEEVWTAEK"
BASE COUNT          798 a         1981 c         1948 g          725 t
ORIGIN      
        1 gcgtcttcat gctgagcccg ttcggccgca tgagcccgtt ccagcaggcg cagatgttca
       61 gcctgcagga cgccaacatc cacaacctga gcgtggaagg cgtgttcgac gactgccagg
      121 acatcgtcaa ggccgtctcg aacgacctcg cgttcaagcg caagtggcgc atcggcaccg
      181 tcaactcgat caactgggcg cggctgctgg cgcaggtcgt ctactacttc gccgggtact
      241 tccaggccac gacgtccaac gaccagaagg tcgcgttcac ggtgccgtcg ggcaacttcg
      301 gcaacgtctg cgccggccac gtcgcacgca tgatgggcct gccgatcgcg cggctcgtgg
      361 tggccaccaa cgagaacgac gtgctcgacg agttcttccg caccggcacc taccgcgtgc
      421 gcggcagcgc cgagacgcac gagacctcga gcccgtcgat ggacatctcc aaggcgtcca
      481 acttcgagcg tttcgtgttc gacctgctgg gccgcgacgg cgggcgtacc cgggacctgt
      541 tcggcgccgg cctgcagcgc gacggcgcat tcacgctgtc ggccgccgac aaggcgcgca
      601 tcgcggaatt cggcttcgtc tcggggcgca gcacgcacgc cgaccgcctg gccaccatcc
      661 gcgacaccga cgcgcgcttc ggcgtcgtca tcgacaccca cacggccgac ggcctgaagg
      721 tggcgcgcga gcacctgctg ccgggcacga cgatgatcgt gctggagacg gcgctgccga
      781 tcaagttcgc cgagacgatc gaggaggcgc tgggccgcaa gcccgcgcta cccgcggggc
      841 tggcggatct ggaatcgcgg ccgcgccgct tcacgaagtt gccggcggac gtcgacgcgg
      901 tgaaggcgtt catcgagaag aacgcgccgg cgcagtaagg ggtctggcct ctcctcgaat
      961 cgcgccgcga cgtcgccgat gacggtggct ctccccgcgt tcgaggacag gcctgacccc
     1021 ggcctccgtt cgaagaccgg ggtcaggcct gtcctctctc cgcgaccgcc ggctggcttc
     1081 gatggtgacc atggcgcgga gttcggaaag gccagacccc tcccatcctg ttgcatcatt
     1141 gactgccatg aacgcacccg acgccaaacc tgccggccgc ggcccgctga tcagcgccga
     1201 cgaggcgctg gcgcgcctgc tgtccgccgt cgtcccgacc ggtcgcagcg agaccgtccc
     1261 cacgcccgag gcctggggcc gcgtgctgtc gcgcgacatc gtctccagcg tccacgtgcc
     1321 gcccgaggac aacagcgcga tggacggcta cgcgctgcgc gtggcggatc tcgcagaggg
     1381 cgccggcagc gtgctgcggg tcagccagcg catcgtcgcc ggacgtcccg gcgagccgct
     1441 gcagccgggc acggcggcgc gcatcttcac cggcgcgcag gttccggcgg gcgccgacgc
     1501 ggtggtgatg caggagcact gcgaggccgt gccgggaccc ggcctgggca cggtgcgcgt
     1561 caacgaggtg ccggcgccgg gcacggcgat ccgccgccag ggcgaggacg tgcggcgcgg
     1621 acacgtggtg ctgcgcgccg ggcatcgcat ggatgcggcc gccatcggcc tggccgcgac
     1681 ggtgggcatg gccacgctgg aggtcgccgc gcggccccgc gtggcgttgt tctccaccgg
     1741 cgacgaactg gtgatgccgg gcgagccgct gccgcccggc gccatctaca actccaaccg
     1801 cttcatgctg ggcgcgctgc tgcgagggca gggctgcgtc gtcaccgacc tcggcaacgt
     1861 gcccgacaag ctcgacgcca cgcgcgccat gctgcgcgag gccgccggcg ggcacgacct
     1921 gatcctgagc tcgggcggcg tctcggtcgg cgaggaagac cacctcaagc cggcgctggc
     1981 cgccgagggc cggcttgacc tctgggcgat cgcgatcaag cctggcaagc cgctggcgtt
     2041 cggcgccgtg cgcaacgggg cggcccaggg cggcgagacc tggttcatcg gcctgccggg
     2101 caacccggtg tccagccacg tcacgttcac gctgttcgtg cggccggtgc tcgcgcggct
     2161 gcagggcgcc gaggcggcct ggccggcggg cgtgccgatg cgggccgact tcgcctggcc
     2221 gcgccccgac aagcgccgcg agttcctgcg cgtgcgccgc aacgcctccg gcggcctcga
     2281 cctgtacgcc aaccagggct ccggcgtcct cacctcgctg gcctggggcg acggcctggc
     2341 cgacctcgcg cctggccagg ccgtgcagcc gggcgacctg gtgcgcttcc tgtcgctggc
     2401 ggagctcgcg ccgtgaagct cacgctcaag tacttcgcct cgctgcgcga gacgctgggc
     2461 gccggcggcc cgctcgagtg gacgcccgag gccgaaccca ccgccgcggc cctgcgcgcg
     2521 tggctgcgca cgcagtcgcc cgcgcacgcg caggcgctcg cgcccgggcg cgccgtgcgc
     2581 gtggccgtcg accaggcgct cgccgacgag tccgccacgc tgcgcgacgg cgcggaagtc
     2641 gcgttcttcc cgccagtcac cggaggctga cgtggcgtcg tcgcgggtgc gcatccagga
     2701 ggccgacttc gatgtcggcg cggagctcgc ggcgctgcag ggcgggcgca ccgacgtcgg
     2761 cgccgtcgcc agcttcacgg gcctcgtgcg cgggcacacg cgcgaggacg acatctccgc
     2821 gctgacgctc gagcactacg ccggcatgac cgagcgcgcc atcgaggcga tgatcgccga
     2881 ggcgcaggcg cgcttcggtc tccaggcggc gctggtcatc caccgcgtcg ggcgcctgct
     2941 gcccggcgag cgcatcgtgc tcgtggccgt ggccgcgccg caccggcgcg cggcctttca
     3001 gggctgcgag ttcctgatgg actacctgaa gacgcacgcg ccgttctgga agaaggagac
     3061 gacgcccgac ggcgagcgct gggtcgacgc ccgcgtggcc gatgacgagg cgctgcggcg
     3121 ctgggggctc cagtcggcga acgcgtcatc gggcacgccc tgaccgaggc cggcgccgcc
     3181 cgagggggct ggctccgcct cgatccatcg aactccgccg gcgcggcgcc aggtacggcg
     3241 acgctcgcgc ggcgccgagc gcacgtcggt tgccctgcga caataggctg ctgccgggca
     3301 aggccccggc tttcttccag cgtcccgttt ccgatgagcg cacccaccga ccccacgtcc
     3361 caccccgccg tgcccgagat cgccgccagc gagctcgtgc ccgacgaact gcgccgcgcg
     3421 ctgggccgct tcgtcacggg cgtgaccatc gtcacctgcc gcgacgagca cggcgcgctg
     3481 gtgggcctga cggccaactc cttcaacgcg ctgtcgctgg acccgccgct ggtgttgtgg
     3541 tcgctgcgcg agtccagttc cacgatcagc gccttcaccg gcgccagcca cttcgcgatc
     3601 aacgtgctgg cggccgacca ggtcgagctg tcgcgccgct tcgcgcggcc gtcgagcgcc
     3661 aagttcgacg ccggcgaatg gacggacggg cagggcgggg cgccgctgct ggccggctgc
     3721 gtcgccgtct tcgaatgccg ccggcgcagc caccatcccg ccggcgacca tgtgctgttc
     3781 atcggcgagg tggagcgcat cggcggctcg gcatccacgc ccctggtcta ccacgcgggg
     3841 cactaccgga cgctgggcga cctcgagtag ccggggcgcg ccgctgcagc ccctcgttcg
     3901 gcgtcgcctt gacgccgcgc attgaaggtt cgcacgccat gcgcgagact cgcgccatgc
     3961 gtccgcgcgc gggggctgga tcggcatggc ccttgccgcc cttcgcttga aaatgcgaca
     4021 gccgcaccca tgtgtgccgc agttcaagag gattttcaag atgcgactcg acaagttgac
     4081 gaccgcgttc cagcaggccc tggccgacgg ccagagcgcc gccgtggcac gcgacaaccc
     4141 gtacgtcgaa ccggcccacg tgctggcggc gatgctggcg cagccggacg gcccgaagtc
     4201 cctgctcgac cgcgcgggtg ccaacaccgc cgcgctgcgg accgcgatgg acaccctggt
     4261 cgccggcctg ccgcaggtgc agggcggcgg gcaggtgtcc ggcagccgcg acctggtgca
     4321 cctgctgcag caggccgaga aggaggccgg caagcgcggc gaccagttcg tggccagcga
     4381 gatgttcctg ctggcgctgg ccgatgccaa gaccgacatc ggcggcatcg tgcgcggcca
     4441 cggcctgacg cgcaaggcgc tggaagccgc catcgacgcc gtgcgcggcg gcgccaccgt
     4501 ggactcggcc gacgccgaag gccagcgcga ggcgctcaag aagtacacgc tggacctgac
     4561 cgagcgcgcg cgctcgggca agctcgaccc ggtcatcggc cgcgacgacg agatccgccg
     4621 cgccatccag gtgctccagc gccgcagcaa gaacaacccg gtgctgatcg gcgagcccgg
     4681 cgtgggcaag accgccatcg tcgagggcct ggcccagcgc atcgtcgcgg gcgaggtgcc
     4741 cgagacgctg aagaacaagc gcgtgctcgt gctggacatg gcgggcctgc tggccggcgc
     4801 caagttccgc ggcgagttcg aggagcgcct gaaggccgtg ctcaaggaag tggcgctcga
     4861 cgagggccgc atcatcctgt tcatcgacga gctgcacacc atggtgggcg ccggcaaggc
     4921 cgagggcgcg atggacgcgg gcaacatgct caagccggcg ctcgcgcgcg gcgagctgca
     4981 ctgcatcggc gcgaccacgc tcgacgagta ccgcaagtac gtcgagaagg acgccgcgct
     5041 ggagcgccgc ttccagaagg tgctggtcga cgagcccagc gtcgaggcga ccatcgccat
     5101 cctgcgcggc ctgcaggaga agtacgaggt ccaccacggc gtggagatca ccgacccggc
     5161 catcgtggcc gcggccgaat tgagtgaccg ctacatcacc gaccgcttcc tgccggacaa
     5221 ggcgatcgac ctgatcgacg aggccgcggc caaggtcaag atcgagatcg actccaagcc
     5281 cgaggtgatg gaccggctcg accgccgcat catccagctc aagatcgagc gcgaggccgt
     5341 caggcgcgag accgacgagg cctcgcagaa gcgcctgggc ctcatcgagg aggagatcgc
     5401 gcgactgggt cgcgagtacg ccgacctcga ggaggtgtgg acggccgaga ag
//
LOCUS       DATCRO010000391         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_769596, whole genome shotgun sequence.
ACCESSION   DATCRO010000391 DATCRO010000000
VERSION     DATCRO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769596"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2109)
                     /locus_tag="VIN75_10585"
     CDS             complement(<1..2109)
                     /locus_tag="VIN75_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY8974654.1"
                     /translation="MVPSAIAVLETLPLNPSGKVDRKALPALDLNAHSAAVYEAPQGE
                     VETAIASIWSELLGVDRVGRQDSFFELGGHSLLAIQLQARLRQAGWPSEMRALFLHPR
                     LADFASAVAQSAEPAATATDPDANAATIPAGCDALEPGMLPLVDLDAAELRRIEAAIP
                     GGAANIQDIYPLAPLQEGMLFHHLMQEQGDAYVISLLLRFDGEARLTRFIDALNQVIA
                     RHDILRTAVVWEELREPVQVVCRAAPLTLEWLPIDDADRAAGRAAERLAAHVDRRAFR
                     IDVRRAPMVRAIAVHDAPSGQWLLQLPSHHLALDHTAEKLMIEEIALILQQRHAELPR
                     PVPYRRYVAQARAGMSATEHEAFFRQLLAGVDELTAPFGLVDARLDGSASEESRLTLP
                     ATLSTELREQARRQGVSAATLFHLAWALVLARTAGRDDVVFGTVLFGRMQGGEEAHRA
                     MGMFINTLPLRVELRGRSVAQGLRETHERLTGLLHHEHAPLSLAQRSSGLPGGTPLFS
                     SLVNYRHSGPRRGEEGADAVWEGITLVGAKERTNYPFTLSVNDRGEVFELVLQVERSV
                     RVGRMQGYLEAAVRGIVDALAAGGQAPLHALNVLGPDESATLTRWSEVPASADAAVPM
                     HRLIERQASIDPQAPALVFGTTTLTRGALNARANRLAHHLIALGVRPDSRVGLAVERS
                     LEMMVGVLAILKAGGAYVPLD"
BASE COUNT          316 a          782 c          737 g          297 t
ORIGIN      
        1 atccagcggc acataggcgc cgccggcttt gagaatggcc agcacgccga ccatcatctc
       61 cagcgagcgc tcgacggcca gacccacgcg cgagtccgga cgaacgccca gcgcgatgag
      121 gtgatgagcc aggcggttcg cacgcgcgtt gagcgctccg cgcgtcagcg tggtcgtccc
      181 gaagaccagc gccggggcct gcgggtcgat cgacgcctgg cgctcgatca accggtgcat
      241 cggcacggct gcgtcagcgg atgccggcac ctcgctccaa cgcgtcaagg tggcggattc
      301 gtccggcccc agcacgttca gcgcatgcag cggcgcttga ccgcccgcgg ccagcgcgtc
      361 gacgatgccg cgcaccgccg cctccaggta gccctgcatc cgccccacgc gcaccgagcg
      421 ctcgacctgc agcaccagtt cgaagacctc tccgcggtca ttgaccgaca gcgtgaacgg
      481 atagttggtc cgttccttcg cgccgaccag cgtgatgccc tcccagaccg catccgcgcc
      541 ctcctcccct cggcgcgggc cgctgtgccg atagttcacc aggctggaga acagcggcgt
      601 tccccccggc agcccgctgc tgcgctgggc cagcgacagc ggcgcgtgct cgtggtgcag
      661 caggccggtg agccgctcat gcgtctctcg taagccttgc gcgacgctgc gcccccgcag
      721 ctcgacccgc aagggcagcg tgttgatgaa catgcccatc gcacgatgcg cctcctcgcc
      781 gccctgcatg cggccgaaca gcaccgtccc gaagaccacg tcgtcgcgcc ccgcggtgcg
      841 cgccagcacc agcgcccagg cgaggtggaa caaggtcgcc gcgctcacgc cctggcgacg
      901 cgcctgctcg cgcaactcgg tcgagagcgt cgccggcagc gtcaggcgcg actcctcgct
      961 cgcgctgcca tccagtcgcg cgtcgacgag gccgaaaggc gcggtcagtt cgtcgacgcc
     1021 cgccagcagc tgccggaaga aggcctcgtg ctcggtcgcg ctcatgccgg cgcgcgcctg
     1081 tgcgacgtag cggcggtacg gcaccggccg gggcagttcg gcatggcgct gctgcaggat
     1141 cagcgcgatc tcctcgatca tcagcttctc ggccgtgtgg tcgagcgcga ggtgatggct
     1201 gggcagctgc agcagccact ggccgctcgg cgcgtcgtgg accgcgatgg cccgcaccat
     1261 gggcgcgcgg cgcacgtcga tgcggaaggc gcggcgatcc acgtgggccg ccagccgctc
     1321 ggccgcccgg cccgctgcgc ggtcggcatc gtcgatcggc agccattcga gcgtcagcgg
     1381 cgccgcgcgg cagacgacct gcaccggctc gcgcagctcc tcccacacga cggcggtgcg
     1441 caggatgtcg tggcgcgcga tgacctgatt caaggcgtcg atgaagcgcg tcagccgcgc
     1501 ctcgccgtca aagcgcagca gcagcgagat cacataggca tcgccctgct cctgcatcag
     1561 atggtggaag agcatcccct cctgcagcgg cgcgagggga tagatgtcct ggatgttggc
     1621 ggcgccgccg gggatcgcgg cctcgatgcg ccgcagctcg gcggcgtcga ggtcgaccag
     1681 cggcagcatg ccgggttcga gcgcgtcaca accggccggg atggtcgcgg cattggcgtc
     1741 ggggtccgtg gcggtggcgg ccggttccgc cgactgggcc acggccgacg cgaagtccgc
     1801 gagccgcggg tggaggaaca gcgcccgcat ctccgacggc caccccgctt gacgcagccg
     1861 cgcctgcaat tggatcgcga gcagcgaatg cccgcccagt tcgaagaagc tgtcctgccg
     1921 gccgacacgg tcgacgccga gcagctcgga ccagatcgat gcgatggcgg tctcgacctc
     1981 gccttgcggc gcctcataga cggccgcgga gtgggcattg agatccagcg ccggcagcgc
     2041 cttgcggtcg accttcccgc tcgggttcag cggcagcgtc tccagcacgg cgatggcgct
     2101 cgggaccatg tactccggca agctcttgcc ga
//
LOCUS       DATCRO010000392         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_555702, whole genome shotgun sequence.
ACCESSION   DATCRO010000392 DATCRO010000000
VERSION     DATCRO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555702"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VIN75_10590"
     CDS             <1..1681
                     /locus_tag="VIN75_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY8974655.1"
                     /translation="SEQNTNIGVEVAMQWNDSFNESVLCFTNNIPQRDGGTHLTGLRA
                     AMTRVINKYIDDNELAKKAKVEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEV
                     RAPVEDIVSRLLTDWLLENPNDAKIICGKIVDAARAREAARKAREMTRRKGVLDGMGL
                     PGKLADCQEKDPALCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKL
                     LSSEAILTLITALGTGIGKGMGGDDFNPDKLRYHRIIIMTDADVDGAHIRTLLLTFFY
                     RQMPELVERGHIYIAQPPLYKVKQGKHEQYLKDGDELSAFLLKVALDGATIEPGEGRP
                     AIAGSTLEDLARKYLLATSVMDRLSNWMDVEALRTIADGLTLNLDDAAQAEASAVALA
                     GALHGAQVSAQFDERTDKHVLRIERQHHGNTKASVITQEFVHGADYEALSTAGVTFKG
                     LLSAEAVVKRGEGERQKESKVADFRGAMAWLMSQAEGAVGRQRYKGLGEMNPEQLWET
                     TMDPNVRRLLKVEIEDAIEADHVFTMLMGDEVEPRRNFIELNALRAANIDV"
     gene            1685..>2135
                     /locus_tag="VIN75_10595"
     CDS             1685..>2135
                     /locus_tag="VIN75_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974656.1"
                     /translation="MAASPRWTPPRAALIDEAALRDGVPACVRWAVGLLVFHAVASGG
                     PIAAALLLGFTSPSLRWQHAFSVAGLAMMVLELAIAALPLWRRPWPRVVLAVQFAFFL
                     GDYVVVGDMGQRFASFPWATTRDVVDWAVQAAALLLLFLPSASWWYRR"
BASE COUNT          372 a          724 c          734 g          305 t
ORIGIN      
        1 ctcggagcag aacaccaaca tcggcgtcga ggtggcgatg cagtggaacg actcgttcaa
       61 cgagtcggtg ctgtgcttca ccaacaacat cccgcagcgc gacggcggca cccacctgac
      121 cggcctgcgc gccgcgatga cgcgtgtcat caacaagtac atcgacgaca acgagctggc
      181 caagaaggcc aaggtcgaga tcgccggcga cgacatgcgc gaagggctgg cctgcgtggt
      241 cagcgtcaag gtgcccgagc cgaagttctc cagccagacc aaggacaagc tggtctccag
      301 cgaggtgcgc gccccggtgg aggacatcgt cagccgcctg ctcaccgact ggctgctcga
      361 gaacccgaac gacgccaaga tcatctgcgg caagatcgtg gacgccgcgc gcgcccgcga
      421 ggcggcgcgc aaggcccgcg agatgacgcg ccgcaagggc gtgctcgacg gcatgggcct
      481 gcccggcaag ctggcagact gccaggagaa ggatcccgcg ctgtgcgaga tctacatcgt
      541 cgagggcgat tcggccggcg gctccgccaa gcagggccgc gaccggaagt tccaggccat
      601 cctgccgctg cgcggcaaga tcctgaacgt ggagaaggcg cgctacgaga agctgctgtc
      661 gtcggaggcc atcctcacgc tgatcaccgc gctgggcacc ggcatcggca agggcatggg
      721 cggcgacgac ttcaacccgg acaagctgcg ctaccaccgc atcatcatca tgacggacgc
      781 ggacgtcgac ggcgcccaca tccgcacgct gctgctgacg ttcttctatc gccagatgcc
      841 cgagctcgtc gagcgcggcc acatctacat cgcgcagccg ccgctgtaca aggtcaagca
      901 gggcaagcac gagcagtacc tcaaggacgg cgacgagctg agcgcgttcc tgctgaaggt
      961 tgcgctcgac ggcgccacca tcgagcccgg cgagggccgt cccgccatcg ccggcagcac
     1021 gctggaagac ctggcgcgca agtacctgct ggccaccagc gtgatggatc gcctgtccaa
     1081 ctggatggac gtcgaggcgc tgcgcaccat cgccgacggc ctgacgctca acctcgacga
     1141 tgccgcccag gccgaggcca gcgcggtcgc cctggccggc gcgctgcacg gcgcgcaggt
     1201 cagcgcgcag ttcgacgagc gcaccgacaa gcacgtgctg cgcatcgagc gccagcacca
     1261 cggcaacacc aaggccagcg tcatcacgca ggagttcgtg cacggcgccg actacgaggc
     1321 gctgagcacc gcgggcgtca ccttcaaggg cctgctcagc gccgaggcgg tggtcaagcg
     1381 cggcgaaggc gaacgccaga aggaaagcaa ggtcgccgac ttccgcggcg ccatggcctg
     1441 gctgatgtcc caggccgaag gcgccgtcgg ccgccagcgc tacaagggcc tgggcgagat
     1501 gaaccccgag cagctgtggg agacgacgat ggacccgaac gtccgccgcc tgctgaaggt
     1561 ggagatcgag gacgccatcg aggccgacca cgtgttcacg atgctgatgg gcgacgaggt
     1621 ggagccgcgc cggaacttca tcgagctgaa tgcgctgcgc gccgccaaca tcgacgtctg
     1681 atccttggcg gcctccccgc gctggacgcc gccgcgtgcc gcgctcatcg acgaagccgc
     1741 gctgcgagac ggcgttccgg cgtgcgtgcg atgggcggtt ggactgctcg tgttccacgc
     1801 ggtcgcgagc ggcggaccga tcgccgcggc gcttcttctg ggcttcacca gtccgtccct
     1861 gcgatggcag catgcgttct cggtcgcggg cctggcgatg atggtgctgg aactggcgat
     1921 cgcggcgctg ccgctgtggc gccgtccctg gccccgcgtc gtgctggccg tccagttcgc
     1981 gttctttctc ggcgactacg tcgtggtcgg tgacatgggg caacgcttcg cgagctttcc
     2041 ctgggcgacg acgcgcgatg tcgtcgactg ggcggtgcag gccgcagcgt tgctgctgct
     2101 gttcctgcct tcagcgtcat ggtggtaccg gcggg
//
LOCUS       DATCRO010000393         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_228905, whole genome shotgun sequence.
ACCESSION   DATCRO010000393 DATCRO010000000
VERSION     DATCRO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228905"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VIN75_10600"
     CDS             <1..138
                     /locus_tag="VIN75_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY8974657.1"
                     /translation="EANGTWRLALRFKQRKADEPVELRAFLQHGKDILSETWTHVIPP
                     R"
     gene            135..2048
                     /gene="mdoH"
                     /locus_tag="VIN75_10605"
     CDS             135..2048
                     /gene="mdoH"
                     /locus_tag="VIN75_10605"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY8974658.1"
                     /translation="MNTSPRSTTMPAIVRSPMLPRAWSGFWNGVLGRAENPLTAEAAA
                     LAARPLAWESAVRVRRRVLVALILIATAFAAMVLASGQRELQHPILQGAQIGLFALLF
                     AWVAAGCFTALMGFWVLVKGDRHTISAADAGDAPLPADGRTALIMPICHEDVATVFAG
                     LRATAASLERSGALHAFDLFVLSDSKDEAVIAEERAAYADLRDSLAGRGNVYYRVRRV
                     RTKKKAGNVADFCRRWGANYKYMVVMDADSVMSGDCLQTLVRLMERHPTAGILQTAPQ
                     AVGHATLHARAQQFAARVTGALFTTGMQYWQLGEAHYFGHNAIIRVKPFMEHCGLAPL
                     AGTGAFSGHILSHDFVEAALMRRAGYHVWLVGDLVGSYEQQPPHLLAELQRDRRWCQG
                     NLQNARLIAEPGLHAVHRGMLATGAMAYVSAPLWLLYVVLGAMLWLMGGNVLFTPQGG
                     LAIGIPGLWAGTLTMLVLPRLLGVAAVFMRRETGRFGGGGKLVASALLEATLAMLLAP
                     LRMVAHTLFVVGAMTGWKLEWKSPPREASDVRWGEAAQRFAPVSLIVAAIGAAMSLTG
                     SSALLWMMPVGLPLVLAVPFTVLTGSTALGERVRQARLLLVPEEASSPTVLRQAWSFA
                     KMSPAALRRLAAG"
     gene            2174..2410
                     /locus_tag="VIN75_10610"
     CDS             2174..2410
                     /locus_tag="VIN75_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8974659.1"
                     /translation="MLIVGIVVGAIARWIMPGAQSMGLLMTGVLGIAGSFIGGFIARL
                     FSKPADGAIVHPAGLILSVVGALILLFVMQKAGF"
BASE COUNT          330 a          923 c          926 g          402 t
ORIGIN      
        1 gaggccaacg gcacctggcg cctggccctc cgattcaagc aacgcaaggc cgacgagccc
       61 gtcgagctgc gcgcgttcct ccagcacggc aaggacatcc tgagcgaaac atggactcac
      121 gtcatacctc cccgatgaac acctcgcccc gttcgacgac gatgcccgcc atcgtgcgca
      181 gcccgatgct gccgcgcgcg tggagcggct tctggaacgg cgtgctgggc cgcgccgaga
      241 acccgctgac ggccgaggcc gccgcgctgg cggccaggcc gctcgcctgg gagagcgccg
      301 tgcgcgtgcg ccggcgcgtg ctggtcgcgt tgatcctgat cgccaccgcg tttgccgcga
      361 tggtgctggc cagcggccag cgcgagctgc agcacccgat cctgcagggc gcgcagatcg
      421 gcctgttcgc gctgctgttc gcatgggtgg ccgcgggctg cttcacggcg ctgatgggct
      481 tctgggtgct ggtcaagggg gaccggcaca cgatctccgc ggccgacgcc ggcgacgcgc
      541 cgctgccggc cgacggccgc accgcgctga tcatgccgat ctgccacgag gacgtcgcca
      601 ccgtgttcgc gggcctgcgc gccacggccg cgtcgctgga acgcagcggc gcgctgcacg
      661 cgttcgacct gttcgtgctg tccgacagca aggacgaggc cgtcatcgcc gaggagcgcg
      721 cggcgtacgc cgacctgcgc gactcgctgg ctggccgcgg caacgtgtac taccgcgtgc
      781 gccgcgtgcg caccaagaag aaggccggca acgtcgccga cttctgccgc cgctggggcg
      841 ccaactacaa gtacatggtc gtgatggacg ccgactcggt catgagcggc gactgcctgc
      901 agacgctcgt gcgcctgatg gaaaggcatc cgaccgccgg catcctgcag accgcgccgc
      961 aggccgtcgg ccacgccacg ctgcacgcgc gcgcgcagca gttcgccgcc cgcgtgaccg
     1021 gcgcgctgtt caccaccggc atgcagtact ggcagctggg cgaggcgcac tacttcggcc
     1081 acaacgcgat catccgcgtc aagccgttca tggagcactg cggcctggcg ccgctggccg
     1141 gcacgggcgc gttctcgggc cacatcctgt cgcacgactt cgtcgaggcc gcgctgatgc
     1201 gccgcgccgg ctaccacgtg tggctggtgg gcgacctggt cggcagctac gagcagcagc
     1261 cgccgcacct gctggccgaa ctgcagcgcg accgccgctg gtgccagggc aacctgcaga
     1321 acgcccgcct gatcgccgag cccggcctgc acgcggtgca ccgcggcatg ctggccaccg
     1381 gggcgatggc ctacgtctcg gcgccgctgt ggctgctgta cgtggtgctc ggcgcgatgc
     1441 tgtggctgat gggcggcaac gtgctgttca cgccgcaagg cggcctggcg atcggcatcc
     1501 cgggcctgtg ggccggcacg ctgacgatgc tggtgctgcc gcgcctgctg ggcgtcgcgg
     1561 ccgtgttcat gcgccgcgag accggccgct tcggcggcgg cgggaagctg gtggcgagcg
     1621 cgctgctcga ggccacgctg gcgatgctgc tggcgccgct gcgcatggtg gcgcacacgc
     1681 tgttcgtcgt cggcgcgatg acgggctgga agctggaatg gaagtcgccc ccgcgggaag
     1741 cgagcgacgt gcgctggggc gaggccgcgc agcgcttcgc gccggtcagc ctgatcgtcg
     1801 cggccatcgg cgccgccatg agcctgaccg gctcgtcggc cctgctgtgg atgatgcccg
     1861 tcggcctgcc gctggtgctg gcggtgccgt tcacggtgct caccggcagc acggcgctcg
     1921 gcgagcgcgt gcgccaggcg cgcctgctgc tggtgcccga ggaggcgtcg tcgcccaccg
     1981 tgctgcgcca ggcctggagc ttcgccaaga tgagcccggc ggcgctgcgc aggcttgcgg
     2041 ctggctgagc ggcagcctca gttctttccc gcatccacgg tcgagcatgt cgcactagag
     2101 ttgtccgcaa ggccgagccc gtcgacgacg ggcgcctgaa ccggaggacc ccaacgtgtt
     2161 tcacctgttg tggatgttga tcgtcggcat cgtggtcggc gccatcgcgc gctggatcat
     2221 gccgggcgcg cagagcatgg gcctgctgat gaccggcgtc ctcggcatcg ccggctcgtt
     2281 catcggcggc ttcatcgccc gcctcttcag caagcctgcg gacggcgcca tcgtgcaccc
     2341 ggcgggcctg atcctgtcgg tggtcggcgc gctgatcctg ctgttcgtga tgcagaaggc
     2401 cggtttctga gaccggcacg acagcgcgct cccttagaat cacggctgcg ccgggcgacg
     2461 tcctggcgcg cagcgggccc gggcatgcct cgggcctttt cattcgcagc ccctggatct
     2521 acaccatgaa ccgctgtgcc ccgttcctgc tcgcgctggc cctgtccggg gtcgcgtcgg
     2581 c
//
LOCUS       DATCRO010000394         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_915646, whole genome shotgun sequence.
ACCESSION   DATCRO010000394 DATCRO010000000
VERSION     DATCRO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915646"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VIN75_10615"
     CDS             complement(<1..400)
                     /locus_tag="VIN75_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY8974660.1"
                     /translation="MTTASHPRDVLFDDGDPTTSLPVCDHYSGVEVRMRKSLELQGEL
                     GPVFDVTLDGEDGAKAGGEVEHAHLIAELVQSTANRFDRVGARIVPRGHPAFEKMLEV
                     LLHDAGERLAYLMIPKPLDAADFEAAAHTVD"
     gene            complement(491..1477)
                     /locus_tag="VIN75_10620"
     CDS             complement(491..1477)
                     /locus_tag="VIN75_10620"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8974661.1"
                     /translation="MSKTPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMEAHSDPMTAFKDTDYALLVGARPRGPGMERA
                     DLLAANAQIFTAQGKALDKVASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLR
                     LDHNRALTQVAQKTGTKVADIEKLAVWGNHSPTMYADYRFATVNGKSVKDLINDQVWN
                     ADTFLPTVGKRGAAIIEARGVSSAASAANAAIDHMRDWALGTQGKWVTMGVPSNGEYG
                     IPKDVMFGFPVTTENGKYKIVEGLAIDEFSQERINKTLKELQDEQAGVSHLL"
     gene            1772..2506
                     /locus_tag="VIN75_10625"
     CDS             1772..2506
                     /locus_tag="VIN75_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8974662.1"
                     /translation="MSSDPSGPAFSPLYQQIKLLITRSLQEGEWKPGEAIPAETDLAA
                     RYRVSQGTVRKAIDELATENLVVRRQGKGTFVATHAEEQIQYRFLRLAPDDAKPRGMA
                     RHFIDCKRMRPPAPIARALDLKGTEGAILVRRTLLQDDIPVVLDEIWLPAGPFKGLTA
                     ERLRDYVGPMYEMFEAEFGVRMIRAEEKIRAVAADAATAEVLAVAEGTPLLSVERLSY
                     TYGDKPVELRRGLYRTDRHYYRNALN"
     gene            2729..3157
                     /gene="sdhC"
                     /locus_tag="VIN75_10630"
     CDS             2729..3157
                     /gene="sdhC"
                     /locus_tag="VIN75_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY8974663.1"
                     /translation="MADAKAIKKRPGQMRLVDATQYRLPPAGWVSILHRVSGLVLIIL
                     MPFVIWMFDTSVTDEVSFDQFRNAFIGGIGFVPAFIVKLAALALIWGFLQHFCAGVRH
                     LYMDFTHTVDKEFGRVSALVAIGAALVLTLVFAYKLFIGY"
     gene            3174..3584
                     /gene="sdhD"
                     /locus_tag="VIN75_10635"
     CDS             3174..3584
                     /gene="sdhD"
                     /locus_tag="VIN75_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY8974664.1"
                     /translation="MSTNYGSKRLVVGAHYGLRDWLVQRATAVIMALFTIVLVVQCLT
                     PGEMSYLKWGAIFAHQWMKVLTFVTIASILWHAWVGVRDVLMDYVQPMAARLVLQVAA
                     IVWLVGCAGWALQVLWRIRECPSPLPSPSASSTS"
     gene            3539..>3838
                     /locus_tag="VIN75_10640"
     CDS             3539..>3838
                     /locus_tag="VIN75_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974665.1"
                     /translation="MSVASSITKRKFDVVIVGAGGSGMRASLQLARAGLNVAVLSKVF
                     PTRSHTVAAQGGIGASLGNMSEDNWHYHFYDTIKGSDWLGDQDAIEFMCREAPNVV"
BASE COUNT          574 a         1299 c         1304 g          661 t
ORIGIN      
        1 cgtccaccgt gtgtgccgct gcctcgaagt cggcggcgtc cagcggcttg gggatcatga
       61 ggtaggccag ccgctcgccg gcgtcgtgca gcagcacttc gagcatcttc tcgaaggccg
      121 gatgcccgcg cggcacgatg cgggcgccga cgcggtcgaa gcggttggcg gtggactgca
      181 ccagctcggc gatcaggtgc gcatgttcca cctcgccgcc cgccttggcg ccgtcctcgc
      241 cgtcgagcgt cacgtcgaac accggcccga gctcgccctg caactccagg ctcttcctca
      301 tgcgcacctc gacgccggag tagtggtcgc agacgggcag cgaggtggtg gggtcgccgt
      361 cgtcgaacag gacgtcgcgc ggatgggagg cggtggtcat cggctggctc ggaaatgaaa
      421 aacgcgcgga cgaggccgcg cgttcaggat agctccatgc ggcgcctcat gcgaggcgcc
      481 ttgcaaggca ttacagcagg tgcgacacgc cggcctgctc gtcctgcagt tccttgaggg
      541 tcttgttgat gcgttcctgg ctgaactcgt cgatggccag gccttcgacg atcttgtact
      601 tgccgttctc ggtggtgacc gggaagccga acatgacgtc cttggggatg ccgtattcgc
      661 cgttggaggg cacgcccatc gtgacccact tgccctgggt gcccagcgcc cagtcgcgca
      721 tgtggtcgat ggcggcgttg gcggccgagg cggccgacga gacgccgcgc gcctcgatga
      781 tggcggcgcc gcgcttgccg accgtcggca ggaacgtgtc ggcgttccag acctggtcgt
      841 tgatcaggtc cttgacgctc ttgccgttca cggtggcgaa gcggtagtcg gcgtacatgg
      901 tgggcgagtg gttgccccac acggccagct tctcgatgtc ggcgaccttg gtgccggtct
      961 tctgtgcgac ctgggtcaac gcgcggttgt ggtccaggcg cagcatggcg gtgaagttct
     1021 cgcgcggcag gctcggggcg ctcttcatcg cgatgtaggc gttggtgttg gcggggttgc
     1081 cgacgaccag caccttgacg ttgcgcgagg cgaccttgtc cagcgccttg ccttgcgccg
     1141 tgaagatctg cgcgttggcg gccagcaggt cggcgcgttc catgccgggg ccgcgggggc
     1201 gggcgccgac gagcagcgcg tagtccgtgt ccttgaacgc ggtcatcggg tcggagtggg
     1261 cttccatgcc ggccagcagc gggaatgcgc agtcgtcgag ctccatcatc acgcccttga
     1321 gcgccttctg ggccttctcg tcggggatct cgagcagttg caggatcacc ggctggtcct
     1381 tgcccagcat ctcgcccgag gcgatgcgga acagcagggc gtagccgatc tggccagcgg
     1441 cgccggtgac ggcgacgcga acgggggtct tgctcatgga aaagtctccg agacgagagg
     1501 aaggacggat ggtggaacgg tgacggcgtc accctttccg ggaactggcg cgggtgcgtc
     1561 cggcgtgcgg ccgggcccgg gacggcaacg cgcagtttac tgcggtcccg gggaaaaacc
     1621 cgcacagggt caatcatctt atgtcttata catgacatgg gaagtcggat gctccggaca
     1681 atgttggacg gcccccgcct gccgtgttgc aatcgcggcg gccgtgcgtc cgtccgcggc
     1741 cctccctgcc cgcgtccccc ttgcccgccc catgtcctcg gatccgtccg gccccgcctt
     1801 cagcccgctg taccagcaga tcaagctgct gatcacgcgc agcctgcagg aaggcgagtg
     1861 gaagcccggc gaggcgatcc ccgccgagac cgacctggcc gcgcgctacc gcgtcagcca
     1921 gggcacggtg cgcaaggcga tcgacgagct ggccaccgag aacctcgtcg tgcgccgcca
     1981 gggcaagggc accttcgtgg ccacgcacgc ggaggagcag atccagtacc gcttcctgcg
     2041 cctggcgcca gacgacgcca agcccagggg catggcgcgc cacttcatcg actgcaagcg
     2101 catgcgcccg ccggcgccga tcgcacgcgc gctcgacctg aagggcaccg agggcgcgat
     2161 cctcgtgcgg cgcacgctgc tgcaggacga catccccgtg gtgctcgacg agatctggct
     2221 gccggccggc ccgttcaagg gcctgaccgc cgagcgcctg cgcgactacg tcggtccgat
     2281 gtacgagatg ttcgaggccg agttcggcgt gcgcatgatc cgcgccgagg agaagatccg
     2341 cgcggtcgcg gccgacgcgg cgacggccga ggtgctggcg gtggccgagg gcacgccgtt
     2401 gctgtcggtc gagcgcctgt cgtacacgta cggcgacaag ccggtcgagc tgcggcgcgg
     2461 cctgtaccgc accgaccgcc actactaccg caacgcgctg aactgaagat gacggcgccg
     2521 aacacccgtt tgccgaccgc ggcccgcacc cgcgtttcgt tgcaaaagcg acgttcggcg
     2581 cgctccaatc ccgacttggg caccgcatgt tgcgttgcca taaaattcgc gggttttacc
     2641 gggccccgac gccttccggc gacggcggca ccgcccaggt ggtgcgccgt cggcgccgcc
     2701 cagacgacaa cgacaaggaa ccacaatcat ggctgacgcg aaggcgatca agaaacggcc
     2761 ggggcagatg cgcctggtcg acgcgaccca gtaccggctc ccgccggccg gatgggtgtc
     2821 catcctgcac cgcgtcagcg gcctcgtcct gatcatcctg atgccgttcg tgatctggat
     2881 gttcgacacc agcgtcaccg acgaggtctc gttcgaccag ttccgcaacg ccttcatcgg
     2941 cggcatcggc ttcgtgccgg cgttcatcgt caagcttgcc gcgctcgcgc tgatctgggg
     3001 cttcctgcag cacttctgcg caggcgtgcg ccacctgtac atggacttca cgcacacggt
     3061 cgacaaggag ttcggccgcg tctccgcgct ggtggccatc ggcgcggccc tcgtgctgac
     3121 gctcgtcttc gcctacaagc tgttcatcgg ctactgatcg ggacccgacc aacatgtcca
     3181 ccaactacgg ttccaagcgc ctcgtcgtcg gcgcccacta cggcctgcgc gactggctgg
     3241 tgcagcgcgc caccgccgtc atcatggcgc tgttcaccat cgtcctggtc gtgcagtgcc
     3301 tgacgcccgg cgagatgagc tacctcaagt ggggcgcgat cttcgcgcac cagtggatga
     3361 aggtgctcac cttcgtcacc atcgcctcca tcctctggca cgcctgggtg ggcgtgcgcg
     3421 acgtgctgat ggactacgtc cagccgatgg ccgctcgcct cgtgctgcag gtggccgcca
     3481 tcgtctggct cgtcggctgc gccggctggg ccctgcaagt cctctggaga atcagagaat
     3541 gtccgtcgcc tcttccatca ccaagcgcaa gttcgacgtc gtgatcgtcg gcgccggcgg
     3601 ctcgggcatg cgtgcctcgc tgcagctggc gcgcgcgggc ctgaacgtgg ccgttctgtc
     3661 caaggtgttc cccacccgct cgcacaccgt cgcggcgcag ggcggcatcg gcgcctcgct
     3721 gggcaacatg agcgaggaca actggcacta ccacttctac gacacgatca agggctccga
     3781 ctggctcggc gaccaggacg cgatcgagtt catgtgccgc gaggcgccga acgtcgtc
//
LOCUS       DATCRO010000395         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_893098, whole genome shotgun sequence.
ACCESSION   DATCRO010000395 DATCRO010000000
VERSION     DATCRO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893098"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VIN75_10645"
     CDS             <1..910
                     /locus_tag="VIN75_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HEY8974666.1"
                     /translation="PSGATFPISQVSNNWDTIIVSFADNAGNGNISFTVDPNAGTDAQ
                     FIADVAAKRKAGKKVVLSVGGQNGSISLDNATMVTNFVNSAYAIIQKYGFDGIDLDLE
                     TGVSQGTAIQTNLVTAMQQLKAKVGPNFYLSMAPEHPYVQGGYVAYGSIWGAYLPIID
                     ALRNDLTEIHVQYYNNGGFTYDNASNQQVQEGTVDGLVAGSLMLIEGFSVAYGTGWHF
                     NGLRPDQVAFGVPSGPSSANTGFVTSTVVANTLNCLTRLQGCNTIKPAKAYPTFRGAM
                     TWSINWDRHDGYNFSVPTRAALNALP"
     gene            complement(992..1657)
                     /locus_tag="VIN75_10650"
     CDS             complement(992..1657)
                     /locus_tag="VIN75_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8974667.1"
                     /translation="MNIVTPDLADRLAAEYVLGTLAGGARRRFDALLPAHPALRHAVD
                     AWEQRLLPMALRTESVQPAPRVWTGIEAQLGWRAPARPSPWRLRFWQAFATLATVAAV
                     VLGTAPRHEPVAAPMIVVLHATKGSETIVAGLSPDHRQLSIQPLQKVALASDQSLELW
                     ALKKDGPPASLGLIAADKLTAIQPRSLPRDTKGLAVSLEPLGGSPTGAPTGPVLFVGD
                     LTL"
     gene            complement(1654..2283)
                     /locus_tag="VIN75_10655"
     CDS             complement(1654..2283)
                     /locus_tag="VIN75_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8974668.1"
                     /translation="MTASALKKADELESRLADWLARCALGDRQAFRQLYDATAPRLLG
                     VVAQLVGRGALAEDLLQDVYVRIWKAAGQYRAGAGSPMAWMAAAARYRAIDHLRSRGA
                     RPEVAVADLPSHGGMDDGDDDPTHRMPDPGPGPAQRVEARSEAEAVQGCLGTLQGSQQ
                     QSISLAYYQGLSHGEIAEHLGAPLGSVKTWVRRGLIALKACLESCGWAA"
     gene            2731..3672
                     /locus_tag="VIN75_10660"
     CDS             2731..3672
                     /locus_tag="VIN75_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8974669.1"
                     /translation="MRSLYRLEAVSQRLARLQAQTEHETLRSTYERMLEKGADRFQVK
                     PAGLPAMDHLYDELPNFHDALDDVKRQIALCQDSRDALEITPILLLGPPGVGKTHFAR
                     EVAELLGTGMGFISMSSLTAGWVLSGASSQWKGARPGKVFETLVDGEYANPVMVIDEI
                     DKARAEHAYDPLGALYSLMEHDTAETFIDEFAEVPIDASQVIWVATANDERAIPEPIL
                     NRMNVYQVQAPDHAAARAIAQRLYAEIRADHDWGQRFDATPRDDVLDRLAELAPREMR
                     RALMTGFGNARLAGCEAIRLDDLPAAGGRRAKIGFTH"
BASE COUNT          619 a         1356 c         1378 g          538 t
ORIGIN      
        1 cccgtcgggc gcgacgttcc cgatctcgca ggtgtcgaac aactgggaca ccatcatcgt
       61 ctcgttcgcc gacaacgcgg gcaacggcaa catcagcttc accgtcgacc cgaacgcggg
      121 caccgacgca cagttcatcg ccgacgtggc cgccaagcgc aaggcgggca agaaggtggt
      181 gctgtcggtg ggcggccaga acggctcgat ctcgctggac aacgcgacga tggtgacgaa
      241 cttcgtcaac agcgcctacg ctatcatcca gaagtacggc ttcgacggca tcgacctcga
      301 cctggagacc ggcgtctcgc aaggcaccgc gatccagacc aacctggtca ccgcgatgca
      361 gcagctcaag gccaaggtgg ggccgaactt ctacctgtcg atggcgccgg agcacccgta
      421 cgtgcaaggt ggctacgtcg cctacggctc gatctggggc gcgtacctgc ccatcatcga
      481 tgcgctgcgc aacgatctga ccgagatcca cgtgcagtac tacaacaacg gcggcttcac
      541 ctacgacaac gcgagcaacc agcaggtgca ggaaggcacc gtggacggcc tcgtcgcggg
      601 cagcctgatg ctgatcgagg gcttcagcgt ggcgtacggc accggctggc acttcaacgg
      661 cctgcgtccc gaccaggtgg ccttcggcgt gccctcgggc cccagctcgg ccaacaccgg
      721 cttcgtgacg tcgacggtgg tcgccaacac gctcaactgc ctgaccaggc tgcagggctg
      781 caacaccatc aagccggcca aggcctatcc gaccttccgc ggcgcgatga cgtggtccat
      841 caactgggac aggcacgacg gctacaactt ctccgtgccg acgcgtgcgg cgctgaacgc
      901 gctgccctga catatcggat gatcatcggg aacgaagggg gcgcccgcgg gcgccccttt
      961 ccttcatgcg cgatccggac gcgccgccgt ttcacagcgt caggtcaccc acgaacagca
     1021 ccggcccggt cggcgcgccc gtcggcgagc cgcccagcgg ttcgagcgag acggccaggc
     1081 ccttggtgtc cctgggcagc gacctgggct ggatggccgt cagcttgtcg gccgcgatca
     1141 ggcccagcga ggccggcggc ccgtccttct tgagggccca cagctcgagg gactggtcgg
     1201 aggcgagcgc caccttctgc agcggctgga tggacagctg gcggtggtcg gggctcaggc
     1261 cggccacgat ggtctcgctg cccttcgtgg cgtgcagcac gacgatcatc ggcgcggcca
     1321 ccggctcgtg gcggggcgcg gtgcccagca cgacggcggc gaccgtggcc aaggtggcga
     1381 aggcctgcca gaagcgcagg cgccacggcg acggccgggc cggcgcgcgc cagcccagct
     1441 gcgcctcgat gcctgtccat acgcgcggcg ccggctgcac cgactcggtc ctgagcgcca
     1501 tcggcaggag ccgctgctcc caggcgtcca ccgcatggcg cagcgccggg tgcgcgggca
     1561 gcagcgcatc gaagcgtcgg cgcgcgccgc cggccagcgt gcccagcacg tattcggccg
     1621 cgaggcggtc ggccagatcg ggggtgacga tgttcatgcc gcccacccgc aactttcgag
     1681 gcaggccttc agcgcgatca ggccacgccg cacccaggtc ttgaccgagc ccagtggcgc
     1741 gccgaggtgc tcggcgatct cgccgtggct cagtccctgg tagtacgcga gcgagatcga
     1801 ctgctgctgc gagccctgca gcgtgcccag gcagccctgc acggcctcgg cctccgagcg
     1861 cgcctcgacg cgctgggcgg gaccggggcc cggatcgggc atgcggtgcg tggggtcgtc
     1921 gtcgccgtcg tccatgccgc cgtgcgacgg caggtcggcg acggccacct cgggacgcgc
     1981 gccgcgcgag cgcaggtggt cgatggcgcg gtagcgcgcg gcggccgcca tccacgccat
     2041 cggcgagccg gcgccggctc ggtactggcc ggcggccttc cagatgcgca cgtacacgtc
     2101 ctgcagcagg tcctcggcca gcgcgccgcg gcccaccagt tgcgcgacga cgcccagcag
     2161 gcgcggcgcc gtggcgtcgt acagttggcg gaaggcctgg cggtcgccca gggcgcagcg
     2221 cgcgagccag tcggccagcc gggactcgag ctcgtccgct ttcttgagcg cggacgcggt
     2281 catggagcga tcgctttcac gaagggggtt cctggttgcg cccgcgggcc gggacggcgc
     2341 gggccagtgt gccgcgcgcg ggcgtctccc ggcaagcggc gcgatgatgg acgggacgga
     2401 agtggtgcgt cgaagctgca tgggtcctgc cacggcgcac cggaacgggc gcggcgacaa
     2461 ggggactacg cggccgggca gggactggat gcgttccgcc cgcgcgcggc cgctcgggcg
     2521 tgacgcgtgt cacgcgcgca agctcgacag cagggcacgg tgtttgctaa aacagcaact
     2581 gaaggcatcc agccccgctg ttcgaagcgt cggcccccgg cgtgtgcgat ccaatgggtt
     2641 ggaccatgcc cgcacttgcc ggaggtcatc ttgattgcag cgaacgacac gtccaccgag
     2701 acccgcgccc cgcggatccc gatcgcccgc atgcggtcgc tgtaccggct ggaggccgtc
     2761 agccagcggc tcgcgcgcct gcaggcgcag accgagcacg agacgctgcg cagcacctac
     2821 gagcgcatgc tggagaaagg cgccgaccgc ttccaggtga agccggccgg cctgccggcg
     2881 atggaccacc tgtacgacga gctgcccaac ttccatgacg cgctcgacga cgtcaagcgc
     2941 cagatcgcgc tgtgccagga cagccgcgac gccctggaga tcacgcccat cctgctgctc
     3001 gggccgccgg gcgtcggcaa gacgcacttc gcgcgcgagg tggccgagct gctgggcacc
     3061 ggcatgggct tcatctcgat gagctcgctg accgccggct gggtgctgag cggtgcctcc
     3121 agccagtgga agggcgcgcg gcccggcaag gtgttcgaga cgctggtcga cggcgaatac
     3181 gccaacccgg tgatggtgat cgacgagatc gacaaggcgc gcgccgagca tgcgtacgat
     3241 ccgctgggcg cgctctactc gctgatggag cacgacaccg ccgagacgtt catcgacgaa
     3301 ttcgccgagg tgccgatcga cgcgagccag gtgatctggg tggccaccgc caacgacgaa
     3361 cgcgcgatcc ccgagcccat cctcaaccgc atgaacgtct accaggtgca ggcgcccgac
     3421 cacgccgcgg cgcgggcgat cgcgcagcgc ctgtacgccg agatccgcgc cgaccacgac
     3481 tggggccagc gcttcgatgc gacgccgcgc gacgacgtgc tcgatcgcct ggccgagctg
     3541 gcgccgcgcg agatgcggcg cgcgctgatg acgggtttcg ggaatgcacg gctggcgggg
     3601 tgcgaggcga tccgcctgga cgacctgccg gcggcgggcg ggcggcgcgc gaagatcggt
     3661 ttcacgcatt gaggtgattg cggcgcgggc atggcggctt acgccttcgg ctagcccgcc
     3721 ctgcgaatgc acgcgattca gcacgtatcc cgtagatacc gtctcgggcg tcaagccttg
     3781 acaggccaat cagcgcgata gggctggcct gtgtgaacga cgccgaagca caattgcgcg
     3841 agcttatgca tggcggcgcc gagggcactc atcttggcct tgccgcgggc c
//
LOCUS       DATCRO010000396         5434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_139320, whole genome shotgun sequence.
ACCESSION   DATCRO010000396 DATCRO010000000
VERSION     DATCRO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139320"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VIN75_10665"
     CDS             <1..574
                     /locus_tag="VIN75_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY8974670.1"
                     /translation="SYAFAGDVTAVAAGTVGGTFNVTVLGQTIVVSDQTRLSDRQSWH
                     WMDDDPQSNPFNITTFQAYLASSASQHVAVKAAKDSGGVLQALSFAIWPASTFSEVAG
                     PVDATPAPVAASASAPGTFSVDGVAVSAPASAIHYAHDGLTAIAAGNEVIASGTFAAG
                     VLGVAATQPFHNFVIDFGAPRAGGDDVPLY"
     gene            complement(660..2612)
                     /locus_tag="VIN75_10670"
     CDS             complement(660..2612)
                     /locus_tag="VIN75_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974671.1"
                     /translation="MPANALLFQLAFSSIVITAFGYRQFNTWTKGESCRATSDSELLA
                     FAPPRSFTPLWRFLATAALYCLTLVVLYLVVFVMLYNGSMSGFEVLEVSGVTQANAWL
                     VALLIVTGLSPMLPVFSNVEQAVREVMHNWAVVPAKAQDMANELASPTTTFELDEAFL
                     RSTVMPRLGKPFTRSDFTEGRAVTVAQKWCRLKMLLLKFAPPDQDGLEAPRFKSPYAT
                     RFVSDCKALERDIHDFAETSPELLRTLGGSPAAVLLSERLDQMQHRLFVLMCCAAFAA
                     ARSVEEVVRYFRKSYGIGVGHVQLSSAPFDPILDTLIAVTLTVLLISLAFTMTHRDSP
                     VHVRPFLWAANAFCVHGAGLLVGWLVFSRRREESETWVDPRDTPVSRRLLLACIVLAF
                     SLAAIPTLLTSVTASLLNGSTKPLLTLTSDALLRTWPWAFLGSATAVATFIHLERTAN
                     GVASLRLRAFSAAAQAGTNITIALVILGVFTRPGEAPPDLLANLALPVYQLVIVLTGA
                     IGVVLGFFLPRVVHGHGLDRRAGVARFPVDGERARATFTYCGRQVPVTVAHLSLSGAV
                     LELAHAHVDVVPGRQGVLTLVDGAAISVHVARLLPPEEDESATVTPRRFAVRHAAMHG
                     PVAAGSGTAGKLHRFLGSLAPQPAAS"
     gene            complement(2820..2969)
                     /locus_tag="VIN75_10675"
     CDS             complement(2820..2969)
                     /locus_tag="VIN75_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974672.1"
                     /translation="MIRKLSSGQYRLYSRKLDPKTGKRRNLGTFDSREQAEKHEREVQ
                     YFKRH"
     gene            complement(3379..3455)
                     /locus_tag="VIN75_10680"
     tRNA            complement(3379..3455)
                     /locus_tag="VIN75_10680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3419..3421),aa:Met,seq:cat)
     gene            3690..4913
                     /gene="ftsY"
                     /locus_tag="VIN75_10685"
     CDS             3690..4913
                     /gene="ftsY"
                     /locus_tag="VIN75_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8974673.1"
                     /translation="MPDVAPTAEPPAPVSVSTPAPPHAPVQAPAPAQAFVPAPAPAQA
                     PAPAPATPPPVAAEPPSAPLDFLKRVFTGAPAPAATPPAPVAPMAPATAPVAEAPREA
                     WFQRLRKGLARTGSGIAQVFTGARIDDELYEELESALLMADAGVRATEHLLKDLKQRV
                     KAAGATEPAQVKALLADAIVDLLSPLERSLMVGEHQPTVIMVAGVNGAGKTTSIGKLT
                     RHLAEAKCKVLLAAADTFRAAAREQLEVWADRNQVEIVSQEGGDPASVTFDSVRAGKA
                     RGCDVVIADTAGRLPTQLHLMEELRKIKRVITKADETAPHEVLLVIDGNTGQNALEQV
                     KSFDAALGLTGLVVTKLDGTAKGGVLAAIALWSRERAAQAHARPIPVYFIGVGEKLED
                     LQTFSAREFASALLN"
     gene            complement(5111..>5434)
                     /locus_tag="VIN75_10690"
     CDS             complement(5111..>5434)
                     /locus_tag="VIN75_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY8974674.1"
                     /translation="AHLFAEATDKFAALGATVVGVSHDDIATQIKFSKEECRDKFAVA
                     ADPESAIIKSYDAKMAVMPYSDRISYVITPDDKVLYVYSSLDPADHVNRTMKAVQDWR
                     ATHKM"
BASE COUNT          834 a         1887 c         1950 g          763 t
ORIGIN      
        1 cagctacgcg ttcgcgggcg acgtcaccgc ggtggcggcc ggcacggtcg gcggcacgtt
       61 caacgtgacg gtgctgggcc agacgatcgt cgtcagcgac cagacgcgcc tgtccgaccg
      121 ccagagctgg cactggatgg acgacgaccc ccagagcaac ccgttcaaca tcaccacgtt
      181 ccaggcctac ctggcctcga gcgcgtcgca gcacgtggcg gtgaaggccg ccaaggactc
      241 cggcggcgtg ctgcaggcgc tgtcgttcgc gatctggccg gcctcgacgt tcagcgaggt
      301 cgccggcccg gtcgacgcga cgcccgcgcc ggtggccgcc tcggcgagcg cgccgggcac
      361 gttctcggtc gacggcgtgg ccgtcagcgc cccggccagc gcgatccact acgcgcacga
      421 cggcctgacc gccatcgccg cgggcaacga ggtgatcgcc tcgggcacct tcgccgcggg
      481 cgtgctgggc gtggccgcga cgcagccgtt ccacaacttc gtgatcgact tcggcgcccc
      541 gcgtgccggc ggcgacgacg tgccgctgta ctgacgaggg cgacgcggct cgcgagggcc
      601 gcgcgcggcg gcgaggcccc tggatccgcg aggagagcag gggccttctg tcttcgggct
      661 caggaggccg cgggctgcgg cgccaggctg cccaggaagc ggtgcagctt gccggcggtg
      721 cccgagcccg ccgcgacggg gccgtgcatg gccgcatggc gcacggcgaa gcgccgcggg
      781 gtcacggtgg ccgactcgtc ctcctcgggc ggcagcagcc gcgcgacatg cacgctgatc
      841 gcggcgccgt cgaccagcgt cagcacgccc tgccttccgg gcacgacgtc gacatgcgca
      901 tgcgccagtt ccagcacggc cccggacagc gacaggtggg cgaccgtcac cggcacctgc
      961 cggccgcagt acgtgaacgt cgcccgcgcg cgctcgccgt cgaccgggaa gcgcgcgacg
     1021 ccggcgcgcc ggtccaggcc atggccatgg acgacccgcg gcaggaagaa ccccagcacg
     1081 acgccgatgg cgccggtcag cacgatcacc agctggtaca cgggcagggc caggttcgcg
     1141 agcaggtcgg gcggcgcctc gcccgggcgc gtgaacaccc cgaggatgac cagtgcgatg
     1201 gtgatgttgg tgccggcctg cgccgcggcc gagaacgccc gcaggcgcag gctcgcgacg
     1261 ccgttggcgg tgcgctcgag gtggatgaac gtcgccacgg cggtggccga accgaggaac
     1321 gcccagggcc acgtccgcag cagcgcgtcg ctggtcaacg tcagcagggg cttggtcgag
     1381 ccgttcagga gcgacgccgt gaccgaggtc aggagggtcg ggatcgcggc caggctgaac
     1441 gcgaggacga tgcacgccag cagcagcctg cggctcaccg gcgtgtcgcg cggatcgacc
     1501 caggtctcgg actcctcgcg ccggcgcgag aacacgagcc agccgaccag cagtcccgcg
     1561 ccgtgcacgc agaaggcgtt cgcggcccac aggaaggggc gcacgtgcac cgggctgtcg
     1621 cgatgggtca tggtgaaggc cagcgagatc agcagcacgg tcagcgtcac cgcgatcagg
     1681 gtgtccagga tcgggtcgaa cggcgcagac gacagctgca cgtgcccgac gccgatgccg
     1741 tagctcttgc ggaagtagcg caccacctcc tcgaccgagc gcgcggcggc gaacgcggca
     1801 cagcacatca gcacgaacag ccggtgctgc atctggtcga gccgttccga cagcagcacc
     1861 gccgccggcg agccgccgag cgtgcgaagc agctcgggcg acgtttcggc gaagtcgtgg
     1921 atgtcgcgct cgagcgcctt gcagtcgctc acgaagcgcg tggcgtaggg cgacttgaag
     1981 cgcggcgcct cgagcccgtc ctggtccggc ggcgcgaact tgagcagcag catcttcagg
     2041 cggcaccact tctgcgcgac ggtgacggcg cggccctcgg tgaagtcgct gcgcgtgaac
     2101 ggcttgccca gccgcggcat gacggtgctg cgcaggaagg cctcgtccag ctcgaaggtc
     2161 gtcgtcggcg acgcgagttc gttggccatg tcctgcgcct tggccggcac caccgcccag
     2221 ttgtgcatca cctcgcgcac ggcctgttcg acgttggaga acacgggcag catcggcgac
     2281 aggccggtga cgatgagcag cgcgaccagc cacgcgttgg cctgcgtgac gccggacacc
     2341 tcgagcacct cgaagcccga catcgatccg ttgtacagca tcacgaacac gaccaggtac
     2401 agcacgacca gcgtcaggca gtacagggcg gccgtggcca ggaagcgcca cagcggcgtg
     2461 aagctgcgcg gcggcgcgaa ggccagcagt tcggagtcgg acgtcgcccg gcacgactcg
     2521 cccttcgtcc aggtgttgaa ctggcgatag ccgaaggcgg tgatgacgat ggacgagaac
     2581 gccagctgga acaggagcgc gttggcaggc atgattcctc tccccctggg gcatgcggaa
     2641 tcgaagccga gcacgctcgt gtcgccgcgc tgcccctggc gcggtcgctc gggctgcgcg
     2701 gcccgtccgg tcgcgcagcc agcatggtga gcgatcgcgc ccgcttttca tcccccgaaa
     2761 ggatgaagcg gcccttcaag ataggggggg ccggcgccgc gcgcgtcggg ggcggccgtt
     2821 cagtgccgct tgaaatattg cacctcgcgc tcgtgcttct cggcctgttc gcgcgagtcg
     2881 aaggtgccga gattgcgccg cttgccggtc ttggggtcga gcttgcgtga atacaggcgg
     2941 tactggccgc tgctgagctt gcggatcatg gggggctcct gaatctgtgg cgtgatcgtc
     3001 gacagggagc agtcgtcgcg cccgcttgcc tgtccgcgcc gatgaagcgg acgagtcatt
     3061 gcagaacgcg ccattccagg agtccaccga atcgatcggg aggagcgtgg acgagacgcc
     3121 gggaggatga aatggcgcgg atggggatgg tgcgaagcgc gcatccattc cgctcgccgt
     3181 gtggacgctt gatcatgtcg ttcgccctga tcgagcacta cgacgccacg gacgcccaga
     3241 tcaagcacat cgtcccgctc aagcgcctct gctggccggc ctgacgacta cctgggcttg
     3301 gcggggcttg gcgcggcggc caagcgcgtc cgccaaggaa aaagcccgca gcgcggatgc
     3361 actgcgggct ttcggttctg gtggcgcttc agggattcga accccggacc tgcggattat
     3421 gattccgtcg ctctaaccgg ctgagctaaa gcgccaagcc tgccattgta tgcgactttt
     3481 tggtggcaag ggaaggggtt ggcggcacaa tgtcgaaaaa gacctttcgg gcttgcatcg
     3541 tgcccgactg ccccgaccat tgaacgtcgc gcgtcccagg ccgggcgcgg gcggcgagtc
     3601 gccgcgatgc ctccttgaga ccgcttcccc tcatgttcag cttcttccgc aagaaatccg
     3661 aatcgggctc cccgtgggag acgcagcgca tgccggacgt cgcgccgacg gccgagccgc
     3721 cggcgcccgt gtcggtatcg acgcccgcgc cgccgcatgc gcccgtccag gcgcccgcgc
     3781 cggcccaggc gttcgtgccg gcacccgcgc ctgcacaggc acctgcgccc gcgcccgcga
     3841 ctccgccgcc tgtcgccgcc gagccgccgt cggcgccgct cgacttcctc aagcgcgttt
     3901 tcaccggcgc ccccgcgccg gccgcgacgc cgccggcccc cgtcgcaccg atggcgccgg
     3961 caacggcgcc cgttgccgag gccccgcgcg aggcctggtt ccagcgcctg cgcaagggcc
     4021 tggcgcgcac gggctcgggc atcgcgcagg tgttcaccgg cgcgcgcatc gacgacgagt
     4081 tgtacgagga gctcgagtcc gcgctgctga tggccgatgc cggcgtgcgc gccaccgagc
     4141 acctgctcaa ggacctgaag cagcgcgtga aggcggccgg cgcgaccgag cccgcgcagg
     4201 tcaaggcgct gctggccgac gccatcgtcg acctgctgtc gccgctggag cgctcgctga
     4261 tggtgggcga gcaccagccg accgtcatca tggtcgcggg cgtcaacggc gccggcaaga
     4321 cgaccagcat cggcaagctc acgcgccatc tcgcggaggc gaagtgcaag gtgctgctgg
     4381 cggcggccga caccttccgc gccgccgcgc gcgagcagct cgaggtctgg gcggaccgca
     4441 accaggtcga gatcgtcagc caggaaggcg gcgacccggc gtcggtgacg ttcgactcgg
     4501 tgcgggccgg caaggcgcgc ggctgcgacg tcgtcatcgc cgacacggcc ggccgcctgc
     4561 cgacgcagct ccacctgatg gaggagctca ggaagatcaa gcgcgtcatc accaaggccg
     4621 acgagaccgc gccgcacgag gtgctgctgg tgatcgacgg caacacgggc cagaacgcgc
     4681 tggagcaggt caagtccttc gacgcggcgc tgggcctcac gggcctggtg gtcaccaagc
     4741 tcgacggcac cgccaagggc ggcgtgctgg ccgccatcgc gctgtggtcg cgcgagcgcg
     4801 ccgcgcaggc gcacgccagg ccgatcccgg tctacttcat cggcgtgggc gagaagctgg
     4861 aggacctgca gaccttcagc gcgcgcgagt tcgcgagcgc gttgctgaac tgacgcggcc
     4921 cgtccggccg tgccctggga cgccccggca ggggtgggtc acgccgtccc tcgcgagatt
     4981 gatcctgcga ctgcgcttcg ctccgcgcag gatgacgcct cggcggtgac gacctggctc
     5041 gccgacggcg ggtctccgga aacgacgacg gggcccgcgg gccccgttct tcatcgcgct
     5101 tcgtggcgcc tcacatcttg tgcgtcgccc gccagtcctg caccgccttc atcgtgcgat
     5161 tgacgtggtc cgcgggatcg aggctggagt agacgtacag caccttgtca tccggcgtga
     5221 tcacgtacga gatgcggtcg gagtagggca tcacggccat cttggcgtca taggacttga
     5281 tgatggccga ctcggggtcg gcggcgacgg cgaacttgtc gcggcactct tccttggaga
     5341 acttgatctg cgtggcgatg tcgtcgtgcg acacgcccac caccgtcgcg ccgagcgcgg
     5401 cgaacttgtc ggtggcctcg gcgaacaggt gcgc
//
LOCUS       DATCRO010000397         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_803071, whole genome shotgun sequence.
ACCESSION   DATCRO010000397 DATCRO010000000
VERSION     DATCRO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803071"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1947
                     /locus_tag="VIN75_10695"
     CDS             241..1947
                     /locus_tag="VIN75_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8974675.1"
                     /translation="MTASPGTGAAAFLAARDFLLQHREDYATAYRDFRWPQLTEFNWA
                     LDHFDVLARGNDAPALWIVDESGAEDKLSFARLAERSNQVANWLRGLGVQRGDRVLLL
                     MPNEVALWESMLACMKLGAVIVPTTTLVSTDDLTDRFDRGGVKHVICAASATSKFDLF
                     PGAYTRVAVGAAAGQAPAGWNDYAASRQAPATFAPDAPTRASDPLFLYFTSGTTSKPK
                     LVLHTHQSYPVGHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATIFIYN
                     YARFNPPAALDMLVRNKVTSLCAPPTVWRMLMQQDLAKYRTSLRELVGAGEPLNPEVI
                     DIIRRAWGITIRDGYGQTETTAQVGNPPGQPVRAGSMGRPLPGYSVVLLDADGRAGDE
                     GEISLSLSPRPTGLMPGYLDDEAKTAEVMRDGHYHTGDVAARDADGYITYVGRADDVF
                     KASDYRISPFELESVLIEHEAVAEAAVIPSPDPIRLAVPKAILILRAGFTPGPELAQS
                     ILAFARGRLASYKRIRIVEFGELPKTLSGKIRRVELRQRENARKAGEAFPLEFREADK
                     AE"
     gene            2158..2694
                     /locus_tag="VIN75_10700"
     CDS             2158..2694
                     /locus_tag="VIN75_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974676.1"
                     /translation="MITARQSRLRYAIVVCAAVVAAVFVAGSSRAAGLDLHARADAKD
                     VGLPLYPGAVKKTDGDGDAGAVSLGIWGGSFGFRLAVVSYRSADSVEKVAAFYREAMG
                     RYGPVLDCTNAHGKSRDEGSGKADRDRPVTCDNEGVQAGGHLYKVGVQHAQRVFKVAP
                     DGTGASFTLVGIELRGED"
     gene            complement(2865..4304)
                     /locus_tag="VIN75_10705"
     CDS             complement(2865..4304)
                     /locus_tag="VIN75_10705"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8974677.1"
                     /translation="MEHLDAIIVGAGLSGIGAAVHLQTDFPGRRFAILESRAAIGGTW
                     DLFRYPGVRSDSDMFTLGYAFKPWTDSKSLADGPAIRQYIVETAVEHGLDRQVRFDHK
                     VVRADWSSADACWTLEIEHAGRRRSRIRCNFLLACSGYYRYSEGHAPEFAGVADYRGR
                     FVHPQFWPSDLEHAGKRVVVIGSGATAVTLVPEMARSAEHVTMLQRSPTYVLSLPGRD
                     PLAERLRRWLPRMLAYRLTRTKNIGVAMLFFNLSRRWPAMVRRNLIGLVRQALGPSHD
                     VDTHFTPRYDPWDQRVCFVPDGDLFEAIKGRRAEVVTDHVDTFTPTGIRLRSGRELTA
                     DIVVSATGLKLNVLGDVAFAVDGKDVDFSKTVGYKGMMFSGVPNLIYTFGYTNASWTL
                     KADLTADYASRLFGYMDRHGYRSAMPVAAPGLETRPFLDFSSGYVQRALEMMPKQGAK
                     APWRLYQNYLLDLLTLRWARIADGRLTFR"
BASE COUNT          641 a         1551 c         1512 g          602 t
ORIGIN      
        1 cgtccctgta gggcgggtta gccgtaggcg taacccgcca tcgcgcgacg cccgccttga
       61 aacgacgcgc gcgcccctgc cgttcattcc cgcgcgctcg aaaaatggct gcgggccctt
      121 gtcaggcggg cgtcggccgg ctcccgtatg ctcgtcgtcc gagaacgccg ccggacggcg
      181 ctccgcccac gacgaccgcc gccatcgcca cccgatggac ggcgcacgga gacacgacgc
      241 atgaccgcat cccccggcac cggcgccgcc gccttcctgg ccgcgcgcga cttcctgcta
      301 cagcaccgcg aggactacgc cacggcgtac cgcgacttcc gctggccgca gctgaccgag
      361 ttcaactggg cgctcgacca tttcgacgtg ctcgcccgcg gcaacgacgc gcccgcgctg
      421 tggatcgtcg acgagagcgg cgccgaggac aagctcagct tcgcgcgcct cgccgagcgc
      481 tcgaaccagg tggccaactg gctgcggggc ctgggcgtgc agcgcggcga ccgcgtcctg
      541 ctgctgatgc ccaacgaggt ggcgctgtgg gaatcgatgc tggcctgcat gaagctgggc
      601 gccgtgatcg tgcccaccac cacgctggtg agcaccgatg acctgaccga ccgcttcgac
      661 cgcggcggcg tcaagcacgt gatctgcgcg gcctcggcca cttccaagtt cgacctcttc
      721 cccggcgcgt acacccgcgt ggcggtgggc gccgccgcgg gccaggcgcc cgccggctgg
      781 aacgactacg ccgcctcgcg ccaggcgccg gccacgttcg cgcccgacgc gccgacccgg
      841 gcgagcgatc cgctgttcct gtacttcacc tcgggcacga cgtccaagcc caagctggtg
      901 ctgcacacgc accagagcta tccggtcggc cacctgagca ccatgtactg gatcggcctg
      961 aagccgggcg acgtgcactg gaacatcagc tcgccgggct gggccaagca cgcgtggagc
     1021 tgcttcttcg cgccgtggaa cgcgggtgcg acgatcttca tctacaacta cgcgcgcttc
     1081 aacccgccgg ccgcgctcga catgctggtc aggaacaagg tgacgtcgct gtgcgcgccg
     1141 cccacggtgt ggcgcatgct gatgcagcag gacctcgcca agtacaggac ctcgctgcgc
     1201 gagctcgtgg gcgccggcga gccgctcaac ccggaggtga tcgacatcat ccggcgcgcg
     1261 tggggcatca ccatccgcga cggctacggc cagaccgaga cgaccgcgca ggtcggcaac
     1321 ccgccgggcc agccggtccg cgccggctcg atgggccggc cgctgccggg ctattccgtc
     1381 gtgctgctgg acgccgacgg ccgcgcgggg gacgagggcg agatcagcct gtcgctgtcg
     1441 ccccgcccca ccggcctgat gccgggctac ctggacgacg aggccaagac cgccgaggtg
     1501 atgcgcgacg gccactacca cacgggcgac gtcgccgccc gcgacgccga cggctacatc
     1561 acctacgtcg gccgcgccga cgacgtgttc aaggcctccg actaccgcat cagcccgttc
     1621 gagctcgaga gcgtgctgat cgagcacgag gcggtggccg aggccgcggt gatcccgtcg
     1681 cccgacccga tccggctggc ggtgcccaag gccatcctga tcctgcgcgc cggcttcacg
     1741 cccggcccgg agctcgcgca gtccatcctc gccttcgcgc gcgggcgcct ggcctcgtac
     1801 aagcgcatcc gcatcgtcga gttcggcgag ctgcccaaga cgctgtcagg caagatccgc
     1861 cgcgtcgagc tgcgccagcg ggagaacgcg cgcaaggccg gcgaggcatt tccgttggag
     1921 ttccgcgagg ccgacaaggc ggaataggac gcgcaggcgg ccggcccgcg cagctcgcaa
     1981 ggccgtcatc ctgcgcgcag acgcaggatc cacccctgcc ggggcgccgc cgtctcccgg
     2041 cgtcccgttc atcgagcgcc gctgcagttc gtccgcgcgc gccgtcggct cgtcgggcgg
     2101 gcgtggcgcc gcagggcggc ggcttccaga atgtcgccag cctgaaggag acgccccatg
     2161 atcaccgccc gccagtcccg cctgcgttac gcgatcgtcg tgtgtgccgc cgtcgtcgcg
     2221 gccgtgttcg tggcgggctc gagccgcgcc gcggggctcg acctgcacgc gcgcgccgat
     2281 gccaaggacg tgggcctgcc gctgtatccc ggcgcggtca agaagaccga tggcgacggc
     2341 gatgccggcg ccgtgtcgct gggcatctgg ggcggctcgt tcggcttcag gctggccgtc
     2401 gtcagctacc gcagcgccga cagcgtcgag aaggtggccg ccttctaccg cgaggcgatg
     2461 ggccggtacg ggccggtgct cgattgcacg aacgcgcacg gcaagtcgcg cgacgaaggc
     2521 tcgggcaagg ccgaccgcga ccggcccgtg acctgcgaca acgagggcgt gcaggccggt
     2581 ggccacctgt acaaggtcgg cgtgcagcac gcgcagcgcg tgttcaaggt ggcgccggac
     2641 ggcaccggcg cgtcgttcac gctcgtcggc atcgagctgc gcggcgagga ctgaacaggc
     2701 ccaaggagtc tgcgcggcag aaggcgcctg ctgcgaacgg cgataagcgg ggccttttca
     2761 gcggcggctc ctctcatgta gcgcccacta cgagttcgtc gccgccgctg aaaagtccat
     2821 gcttctcgcc gctctcgcga cggcgccttc cgccgcgcag actcctagcg gaacgtcagc
     2881 cgcccgtccg cgatgcgcgc ccagcgcagc gtcagcaggt ccagcaggta gttctggtag
     2941 agccgccacg gcgccttggc gccctgcttg ggcatcatct ccagcgcacg ctgcacgtag
     3001 cctgacgaga agtccaggaa ggggcgcgtc tcgaggccgg gggcggcgac gggcatcgcg
     3061 ctgcggtagc catgccggtc catgtagccg aacagccgcg acgcgtagtc ggccgtcagg
     3121 tcggccttga gcgtccacga ggcgttcgtg tagccgaagg tgtagatcag gttgggcacg
     3181 ccgctgaaca tcatgccctt gtagcccacg gtcttcgaga agtcgacgtc cttgccgtcc
     3241 acggcgaacg cgacgtcgcc cagcacgttc agcttcaggc ccgtggccga gacgacgatg
     3301 tcggcagtca gctcgcggcc cgaacgcagg cggatgccgg tcggcgtgaa ggtgtcgacg
     3361 tggtcggtga cgacctccgc gcgcctgccc ttgatcgcct cgaacaggtc gccgtcgggg
     3421 acgaagcaca cgcgctggtc ccacgggtcg tacctcggcg tgaaatgggt gtcgacgtcg
     3481 tgcgacgggc cgagcgcctg gcgcacgagc ccgatcaggt tcctgcgcac catcgccggc
     3541 cagcgccgcg acaggttgaa gaacagcatc gccacgccga tgttcttggt gcgcgtgagc
     3601 cggtacgcga gcatgcgcgg cagccagcgg cgcaggcgct ccgcgagcgg atcgcggccc
     3661 ggcagcgaca gcacgtaggt cggcgagcgc tgcagcatcg tcacgtgttc ggcgctgcgc
     3721 gccatctcgg gcaccagcgt caccgcggtg gcgccgctgc cgatgacgac cacgcgcttg
     3781 ccggcgtgct cgaggtcgga cggccagaac tgcggatgca cgaagcggcc ccggtagtcg
     3841 gcgacgccgg cgaactcggg cgcgtggccc tcgctgtagc ggtagtagcc gctgcacgcg
     3901 agcaggaagt tgcagcggat gcgcgagcgg cgccggccgg catgctcgat ctcgagcgtc
     3961 cagcacgcgt cggccgacga ccagtccgct cgcaccacct tgtggtcgaa gcgcacctgg
     4021 cggtcgaggc cgtgctcgac ggccgtctcg acgatgtact ggcggatggc cggcccgtcg
     4081 gccagcgact tcgagtccgt ccacggcttg aaggcgtagc cgagcgtgaa catgtcggag
     4141 tcggagcgca cgcccggata gcggaacagg tcccacgtgc cgccgatcgc cgcgcggctt
     4201 tccaggatcg cgaagcggcg gccggggaag tcggtctgca ggtgcacggc cgcgccgatg
     4261 cccgacagcc cggcgccgac gatgatcgcg tcgaggtgct cgatcg
//
LOCUS       DATCRO010000398         6572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1061791, whole genome shotgun sequence.
ACCESSION   DATCRO010000398 DATCRO010000000
VERSION     DATCRO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061791"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VIN75_10710"
     CDS             <1..1287
                     /locus_tag="VIN75_10710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY8974678.1"
                     /translation="LVSGACLAEMGNHVVCLDVDPRKIDMLNKGEIPIHEPGLDQVVQ
                     RNAAAGRLQFTTDIGVAVAHGTLQFIAVGTPPDEDGSADLQYVLAAARNIGRLMTDYK
                     LVIDKSTVPVGTADKVKAAIQGELDARGLALDFSVASNPEFLKEGAAVDDFMKPDRIV
                     VGADDDRAINLLRAVYAPFQRNRDKLVVMDVRSAELTKYAANAMLATRISFMNELALL
                     AENLGADIELVRQGIGSDPRIGYHFLYAGCGYGGSCFPKDVKALIRTASESGRDLKVL
                     QAVEDANDAQKQVLVGKIVKRFGDDLAGKRFAVWGLAFKPNTDDMREASARVLIGELL
                     ARGASVTAYDPVAMNEARRIFGDEPRIAYAENPDEALVGADALAIVTEWKEFRSPDFA
                     RIATTLKTPVIFDGRNLYEPRIVRDAGIDYQPIGRA"
     gene            1353..2444
                     /locus_tag="VIN75_10715"
     CDS             1353..2444
                     /locus_tag="VIN75_10715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8974679.1"
                     /translation="MRSTATTATAVCAVLLLAACTGHKKDTTQSAARVDGTEITVHQI
                     NYKLQRERGLRPEQQEEAGRKVLEQLIDEQLIVEKAEKTKMDLEPGAQQALDAARREV
                     LARAYVDITAQSVTPPSDAAVHAYYDANPYLFGQRRVYTLQEYLAKVPEDKIAALRAM
                     VEAGRPPEETEAWFKAQGAPFRAQQSTQPAERIPMGSLKEIAVIPVGHGLVASAGDQV
                     HVTYVTSSTDAPAPFDVARPKIMQFLAIESRKKVTEGNIAALKTAAKIEYAPQYAALA
                     ASGAALATTKDIESTPSPLTTASGTHVSLPAASEASSVQVTLPTTTSSSVQVSLPQSQ
                     GVRVSLPATPASSVQVRLPAPASQDATKK"
     gene            2456..3340
                     /gene="epsE"
                     /locus_tag="VIN75_10720"
     CDS             2456..3340
                     /gene="epsE"
                     /locus_tag="VIN75_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HEY8974680.1"
                     /translation="MLNLVLSRFTRGALRVVAIALSAVALVGTSLADAQAAQQQKPAA
                     PAARAADPDQSRIGPGDTIKITVYQSPDLSMETRVNETGDISYPLLGRVKLAGLSVTQ
                     AEQHIASELKRREFVRDPQVQIVVTQVRANQVNVLGQVGRPGRYPLDLTGMRLTEVLA
                     LAGGIVAGVGSDTVVVTGTRDGKPFRKDVDMPSLFRAGNGAEDIVIAPGDSIFVDRAP
                     QIYLYGEIQHPGALRLERGMTVMQAVAAGGGATPRGTLRGIKITRTGPDGKQTQIEPA
                     MDDQLKNGDVVYIKESLF"
     gene            3357..4784
                     /locus_tag="VIN75_10725"
     CDS             3357..4784
                     /locus_tag="VIN75_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY8974681.1"
                     /translation="MNLTHFLAVLRARWVSAAIVFAAVLVAAVLYLVFATRVYTATAS
                     VLIDAKPDPVSTMLYGGATPALLNTQIDIMRSDRVAQRVVQNLKLADLADMRAAWASA
                     KSGMTLQEYLVDFVEAGLDPKVSNPGSNVINIGYKSPDARFAATVANAYVQAYMETSI
                     ELRVDPARQYSGFFTDQQKEARAALEAAQAKLSAFQKEKGIIGSDDRFDLEMSRLTAL
                     TQELVAIQTARVDASTRQSQIGSAGQLSEVLANGTVSGLKNDLAQAELKLQELSSRYG
                     DKHPQVQEARSAVAQLKAKLAHATSDVTGAIGIDARVSRAREADIQAAVDAQRAKVLQ
                     LKETRDQVAVLQRDVDNAQRTYDQVYSRASATNLESQNRQASATVISQATTPVLPSSP
                     KVPSVLVLGLVAALALGIGTALLQEQFDKRMRTTSDAFDFLGLPVIGIMPSPNMNRRL
                     KGQMAMIQDRVVSGRRLPAPEKGQA"
     gene            4781..5680
                     /locus_tag="VIN75_10730"
     CDS             4781..5680
                     /locus_tag="VIN75_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEY8974682.1"
                     /translation="MNAKMEAPDLYLGDSQSFESTTKTKSIGELIQDARNLSADDLDR
                     IGRYQRENDMRFGEAAIALGLADTEDVLQALAQQFKYAYADEETRKAAPELVMLNQPF
                     GHQAEAFRAIRSQILMRTQGEKGTRRPLAVVSHDTGDGKTFFCANLAVALAQLGGKVV
                     VIDADMRGARLDQVFGVPGGVGLSGVLSGRGGKGVVKSVPGIPNLYVIPVGVQPPNPL
                     ELVEGPSFGMLLREVSIKFDHVIVDTPAGCFGSDGVVIASRCGSALVISRKDANKVAR
                     LQELVGSLQAGNVRLAGVIMNEL"
     gene            5688..6569
                     /gene="xrtB"
                     /locus_tag="VIN75_10735"
     CDS             5688..6569
                     /gene="xrtB"
                     /locus_tag="VIN75_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase B"
                     /protein_id="HEY8974683.1"
                     /translation="MTLVDRLSAPRTWPWWICVLGLLALYVPTLWDLSFNIWLASDEQ
                     SQGPIVLAICIWLIWRSWDRIDDEAASRPRPLAGWALVVLALLLYTFGRSQRILSAQT
                     LSAIFMLPGLMLLLRGPRQLRAGAFALFFMIFMIPLPATFVDAISQPLKLAVSSVASS
                     VLYGVGYPIARTGVILQIGQYQLLVADACAGLHTLFSLEAMGLLYLNVVRHTSLLRNI
                     ALAILIVPISFAANVIRVMVLALITYHFGDEAGQGFLHGFAGLVLFMTALMFIIATDS
                     GLRWLAVRTHPARPQHV"
BASE COUNT         1092 a         2368 c         2193 g          919 t
ORIGIN      
        1 ttggtgtccg gagcctgcct ggcggagatg ggcaaccacg tcgtctgcct ggatgtggat
       61 ccgcgcaaga tcgacatgct caacaaaggc gagatcccca tccacgagcc gggcctcgac
      121 caggtggtgc agcgcaacgc cgccgcgggc cgcctgcagt tcacgaccga catcggcgtg
      181 gccgtcgccc acggcacgct gcagttcatc gcggtgggca cgccgcccga cgaggacggc
      241 tccgccgacc tgcagtacgt gctggccgcc gcgcgcaaca tcggccgcct gatgaccgac
      301 tacaagctgg tcatcgacaa gagcaccgtg cccgtgggta ctgccgacaa ggtcaaggcc
      361 gcgatccagg gcgagctcga tgcgcgcggc ctggcgctcg atttctccgt cgcctccaat
      421 cccgagttcc tcaaggaagg cgcggcggtc gacgacttca tgaagcccga tcgcatcgtg
      481 gtgggcgccg acgacgatcg cgcgatcaac ctgctgcgcg cggtctacgc gccgttccag
      541 cgcaatcgcg acaagctcgt cgtgatggac gtgcgatccg ccgaactgac caagtacgcc
      601 gccaacgcga tgctggccac gcgcatctcg ttcatgaacg aactcgcgct gctggccgag
      661 aacctgggcg ccgacatcga gctcgtgcgc cagggcatcg gctcggaccc tcgcatcggc
      721 taccacttcc tgtacgccgg ttgcggctat ggcggctcgt gcttccccaa ggacgtcaag
      781 gcgctgatcc gcacggccag cgagagcggc cgcgacctga aggtgctgca ggccgtcgaa
      841 gatgccaacg acgcgcagaa gcaggtgctg gtgggcaaga tcgtcaagcg cttcggcgac
      901 gacctggccg gcaagcgctt cgccgtctgg ggcctcgcgt tcaagcccaa caccgacgac
      961 atgcgcgagg ccagcgcccg cgtgctgatc ggcgagctgc tcgcgcgcgg cgccagcgtg
     1021 actgcctacg acccggtggc gatgaacgag gcgcgccgca tcttcggcga cgagccgcgc
     1081 atcgcctacg ccgagaatcc cgacgaggcg ctggtgggcg ccgacgcgct ggccatcgtc
     1141 accgagtgga aggaattccg cagcccggac ttcgcgcgca tcgccacgac gctcaagacg
     1201 ccggtcattt tcgacggccg caacctgtac gagccgcgca tcgtgcgcga cgcgggcatc
     1261 gactaccagc ccatcgggcg cgcctgaccg gcgacatgga ccgcacgcgg ccgcagggga
     1321 atgtgagact catgacgaac aacgagacga cgatgcgttc caccgccacg accgccaccg
     1381 cggtctgcgc ggtgctgctg ctggcggcgt gcaccggtca caagaaggac accacgcaga
     1441 gcgccgcgcg cgtggacggc accgagatca cggtgcacca gatcaactac aagctgcagc
     1501 gcgagcgcgg cctgcgtccc gagcagcagg aagaggccgg ccgcaaggtg ctcgagcagc
     1561 tgatcgacga gcagctgatc gtcgagaagg ccgagaagac caagatggac ctggagccgg
     1621 gcgcgcagca ggcgctcgac gcggcgcgcc gcgaggtgct ggcgcgcgcc tacgtggaca
     1681 tcaccgcgca gagcgtcacc ccgccgtccg acgccgccgt ccacgcctac tacgacgcca
     1741 acccctacct gttcgggcag cgtcgcgtct acacgctgca ggaatacctg gccaaggtgc
     1801 ccgaggacaa gatcgcggcg ctgcgcgcga tggtcgaggc cggccggccg cccgaggaga
     1861 ccgaggcgtg gttcaaggcc cagggcgcgc cgttccgcgc ccagcagagc acgcagccgg
     1921 ccgagcgcat cccgatgggc tcgctcaagg agatcgccgt catccccgtg ggccacggcc
     1981 tggtggcgtc cgccggcgac caggtgcacg tgacctacgt gacctccagc accgacgcgc
     2041 ccgcgccgtt cgacgttgcg cgccccaaga tcatgcagtt cctcgcgatc gagagccgca
     2101 agaaggtcac cgagggcaac atcgcggcgc tcaagacggc cgccaagatc gagtacgccc
     2161 cgcagtacgc ggcgctggcc gcctccggcg cggcgctggc caccaccaag gacatcgagt
     2221 ccacgccctc ccccctgacc accgcgagcg gcacgcacgt ctcgctgcct gccgcaagcg
     2281 aggcgtcgag cgtccaggtg accctgccga cgacgacgtc ctccagcgtg caggtgagcc
     2341 tgcctcagtc gcagggcgtg cgcgtcagct tgccggccac cccggcctcc tccgtgcaag
     2401 tgcgcctgcc ggcgcccgca tcgcaggacg cgaccaagaa atgaaggagc actccatgct
     2461 gaaccttgtc ctgtcccgat tcacccgcgg cgccttgcgc gtcgtggcca tcgccctgtc
     2521 ggcggtggcg ctggtcggca cgtccctcgc ggacgcgcag gccgcgcagc agcagaagcc
     2581 ggccgcgccg gccgcgcgcg ccgcggaccc cgaccagtcg cgcatcggcc ccggcgacac
     2641 gatcaagatc accgtgtacc agagcccgga cctcagcatg gagacgcgcg tcaacgagac
     2701 cggcgacatc agctacccgc tgctgggccg cgtcaagctc gccggcctga gcgtgaccca
     2761 ggccgagcag cacatcgcct ccgagctcaa gcgccgcgag ttcgtcaggg acccgcaggt
     2821 gcagatcgtg gtgacgcagg tgcgcgccaa ccaggtcaac gtgctgggcc aggtcggccg
     2881 gcccggccgc tatccgctcg acctgaccgg catgcgcctg accgaggtgc tggcgctggc
     2941 gggcggcatc gtcgccggcg tgggttcgga caccgtcgtc gtcaccggca cgcgcgatgg
     3001 caagccgttc cgcaaggacg tcgacatgcc cagcctgttc cgtgccggga acggcgccga
     3061 agacatcgtg atcgccccgg gcgattccat cttcgtcgac cgcgcgccgc agatctacct
     3121 gtacggcgag atccagcacc cgggcgcgct gcgcctggag cgcggcatga cggtgatgca
     3181 ggccgtcgcg gccggcggcg gcgccacccc gcgcggtacg ctgcgcggca tcaagatcac
     3241 gcgcaccggc ccggacggca agcagacgca aatcgaaccc gcgatggacg accagctgaa
     3301 gaacggcgac gtcgtctaca tcaaggaaag cctgttctga ggacgggagc ccaaccatga
     3361 atctcactca tttcctcgcc gtcctccgtg cacggtgggt gagcgcggcg atcgtgttcg
     3421 ccgccgtgct ggtggccgcg gtgctgtacc tcgtcttcgc gacgcgcgtc tacaccgcca
     3481 ccgcctccgt gctcatcgac gccaagcccg acccggtcag cacgatgctg tacggcggcg
     3541 ccacgcccgc gctgctgaac acgcagatcg acatcatgcg cagcgaccgt gtcgcccagc
     3601 gcgtcgtgca gaacctcaag ctcgccgacc tggccgacat gcgcgccgcc tgggcctcgg
     3661 ccaagagcgg catgacgctg caggagtacc tggtcgactt cgtcgaggcc ggcctcgacc
     3721 cgaaggtgag caaccccggc agcaacgtca tcaacatcgg ctacaagtcg ccggacgcgc
     3781 gcttcgccgc caccgtcgcc aacgcgtacg tgcaggccta catggagacg tcgatcgagc
     3841 tgcgcgtcga tccggcgcgc cagtacagcg gcttcttcac cgaccagcag aaggaagcgc
     3901 gcgccgcgct cgaggcggcg caggccaagc tgtcggcgtt ccagaaggaa aagggcatca
     3961 tcggctccga cgaccgcttc gacctcgaga tgagccgcct gacggccctc acgcaggagc
     4021 tcgtcgcgat ccagaccgcg cgcgtcgacg cctcgacccg ccagagccag atcggcagcg
     4081 cggggcagct gtccgaggtg ctggccaacg gcaccgtcag cggcctgaag aacgacctcg
     4141 cgcaggccga gctcaagctg caggagctgt cctcgcgcta cggcgacaag cacccgcagg
     4201 tgcaggaagc gcgctcggcg gtcgcccagc taaaggccaa gctcgcgcat gccacgagcg
     4261 acgtgacagg cgccatcggc atcgacgcgc gcgtcagccg cgcccgcgag gccgacatcc
     4321 aggccgccgt cgacgcgcag cgcgccaagg tgctgcagct caaggagacg cgtgaccagg
     4381 tcgccgtgct ccagcgcgac gtcgacaacg cccagcgcac gtacgaccag gtctacagcc
     4441 gcgccagcgc gaccaacctg gagagccaga accgccaggc cagcgccacc gtcatcagcc
     4501 aggccacgac gccggtgctg ccgtcgtcgc ccaaggtgcc gtcggtgttg gtgctgggcc
     4561 tggtcgccgc gctggcgctg ggcatcggca ccgcgctgct gcaggaacag ttcgacaagc
     4621 gcatgcgcac cacgtcggac gccttcgact tcctcggcct gccggtgatc ggcatcatgc
     4681 cgagcccgaa catgaaccgt cgtctcaagg gacagatggc catgatccag gatcgcgtcg
     4741 tctcagggcg acgacttccg gcgccagaaa aggggcaagc atgaacgcca agatggaagc
     4801 acccgatctc tacctcggcg acagccagag cttcgagagc acgaccaaga ccaagtcgat
     4861 cggcgagctg atccaggacg cccgcaacct gtcggcggac gacctcgacc ggatcggccg
     4921 ctaccagcgc gagaacgaca tgcgcttcgg cgaggccgcc atcgcgctgg gcctggccga
     4981 caccgaggac gtgctgcagg cgctcgcgca gcagttcaag tacgcgtacg ccgacgagga
     5041 gacgcgcaag gccgcgccgg aactggtgat gctgaaccag ccgttcggcc accaggccga
     5101 ggcgttccgc gccatccgca gccagatcct gatgcgcacg cagggcgaga agggcacgcg
     5161 ccggccgctg gccgtcgtca gccacgacac cggcgacggc aagaccttct tctgcgccaa
     5221 cctggccgtc gcgcttgcgc agctgggcgg caaggtggtg gtcatcgacg ccgacatgcg
     5281 cggcgcgcgc ctggaccagg tgttcggcgt gccgggcggc gtgggcctgt cgggcgtgct
     5341 gtcgggccgc ggcggcaagg gcgtcgtcaa gtcggtgccg ggcatcccca acctgtacgt
     5401 gatccccgtc ggcgtgcagc cgcccaaccc gctggagctc gtcgagggcc ccagcttcgg
     5461 catgctgctg cgcgaggtct cgatcaagtt cgaccacgtc atcgtcgaca cgccggccgg
     5521 gtgcttcggc agcgacggcg tggtgatcgc gtcgcgctgc ggctccgcgc tggtcatctc
     5581 gcgcaaggat gccaacaagg tcgcccgcct gcaggagctc gtcggttcgc tccaggccgg
     5641 caacgtgcgc ctggccggcg tgatcatgaa cgagctgtga cccggcgatg acgctcgtcg
     5701 accggctcag cgcgccgcga acctggccgt ggtggatctg cgtcctcggc ctgttggcgc
     5761 tctacgtccc gacgctgtgg gacctgtcgt tcaacatctg gctggcgagc gacgagcaga
     5821 gccaggggcc gatcgtcctg gcgatctgca tctggctgat ctggcgctcg tgggatcgga
     5881 tcgacgacga ggcggccagc cgcccgcggc cgttggcggg ctgggccctg gtcgtgctcg
     5941 cgctgctgct ctacacgttc ggccgctcgc agcgcatcct cagcgcgcag acgctctcgg
     6001 ccatcttcat gctgccgggc ctgatgctgc tgctgcgcgg cccgcgccag ctgcgcgccg
     6061 gcgcattcgc gctgttcttc atgatcttca tgatcccgct gccggcgacc ttcgtcgacg
     6121 cgatctcgca gccgctgaag ctggccgtct cgagcgtggc ctcgtcggtg ctgtacggcg
     6181 tcggctatcc gatcgcgcgc accggcgtga tcctgcagat cggccagtac cagctgctgg
     6241 tggccgacgc ctgcgccggc ctgcacacgc tgttctcgct ggaggcgatg gggctgctgt
     6301 acctcaacgt ggtgcggcac acctcgctgc tgcgcaacat cgcgctggcg atcctgatcg
     6361 tgccgatctc gttcgccgcc aatgtcatcc gcgtgatggt gctggcgctg atcacgtacc
     6421 acttcggcga cgaggccggg cagggcttcc tgcacggctt cgccgggctc gtcctgttca
     6481 tgacggcgct catgttcatc atcgccacgg acagcggcct gcgctggctg gccgtgcgca
     6541 cccatcctgc gaggccgcag catgtctgaa cc
//
LOCUS       DATCRO010000399        13289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1039351, whole genome shotgun sequence.
ACCESSION   DATCRO010000399 DATCRO010000000
VERSION     DATCRO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13289
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039351"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VIN75_10740"
     CDS             complement(<1..228)
                     /locus_tag="VIN75_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974684.1"
                     /translation="MIAKSRTIAAKRPSPTVLAVRAGALTLLAGAALALLAHGGARAA
                     DAPAADTSDQVIVPQVERREVHKPKYPSNDFE"
     gene            complement(239..1210)
                     /locus_tag="VIN75_10745"
     CDS             complement(239..1210)
                     /locus_tag="VIN75_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY8974685.1"
                     /translation="MRAVSTSSTALQRAVVLLAFALAGAGTRAADAPAPADAASAPQA
                     AAVPGDAASAPAAVDDRPRTATVPVPGSTQTVEIPAPVTTGSPPTVPVADSVPGGDKR
                     ERVQVTDPYIELHTFAGRGYPVFYVAARGEWISIELRHTDWYKVRTATGKVGWVTRQQ
                     LQSTLAEGGLPNDFGDVKYDDYLARRVELGAGYGRFHRESMIKFWVSYRISDTLSFES
                     TYGQVQGVFSGTTLWHVNLQAEPWSDQRFSPFLGIGIGRFNNIPNQSLVNFQPTNARL
                     ADAIIGARYHLTDRFMVRADYAIYTAFVSDQRSLEYRAATAGLSFFF"
     gene            complement(1291..2010)
                     /locus_tag="VIN75_10750"
     CDS             complement(1291..2010)
                     /locus_tag="VIN75_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974686.1"
                     /translation="MQKQKTRVASCGFAQDDMGAGRAAARAPSRRRARLRRAALGALA
                     AGGLLAGCGGGGDPLSNPGNVQNPSTTGGATLAFPYFQKCIFPIFLKQLPITQNGVTS
                     TNTCAGAGCHDSNTGTGGALRVIVAATPIADADVAAAATDSAMATTIRASDMYKNFYS
                     AQGTTVFGSPIQSRLFDKPLVLNVLHGGGRIFTSQDDPNAKLIDYWISHPVPAGQDEF
                     STSSYSMFTPADPATGTCNTQ"
     gene            complement(2013..5027)
                     /locus_tag="VIN75_10755"
     CDS             complement(2013..5027)
                     /locus_tag="VIN75_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974687.1"
                     /translation="MKLPISFTTRTLLGGASALAGAGVLSLAACGGGSKTESNTVTVQ
                     GDVPIVYAKRVNTIGLNPTNGAPTAPGGDLMIREKSSPSAPEHNITAQFTMGKGDAAT
                     PEVSWDGKKILFSMRCPADNAVQIGGAAACTGHWNVWEYDMTTGGLTGGTFRRITSST
                     ADDDIHPVYLPAGQGIVFSSNRQTESKANQALGHSYYALDEYERERVFNLHTMAMDGS
                     NITQISFNQSHDRNPTIRPNGQIMFSRWDHVGGRNHFKVFTVNPDGTDMFVLYGAHSD
                     GNSFLHPRDMDPKGKYAGFLTSDLMPLQRTHEGGALMLIDAANYSEQNTPAKKSVAAA
                     GGQQQATTQTVNIDRGVSMYGRFTGPYPLWDGTDRVLVSYRPCEVTKNGTVVPCSTLT
                     ADELAKLGDMNRPLAESQADPVQDNVPPPYAIYMFNPANQSMLPVATPPAGFMYVDAV
                     AIQARTEPTTVAAAPLDETLASQKMGTLDVRSVYDTDGLGRMGEGMLTAADANAGCTG
                     PLIPQTAPIDVNDTRASVADINKMKDPANAAYNCAPARFIRAVRAVAPPTSMMKMRSS
                     LGETDFEAQQVLGYAPIEPDGSFKLNVPADTPIALAVVDSQGRAFQTHTNWIQVRPGE
                     RRTCDGCHSPRRGAPINSGATVNTVPAAILAAMANAHQSGETMADTRVRLDPTAAQLK
                     PDMVSTDIWADTTKAGVTARPAVALRYTGNPNPSDDLVTPVPVNGVINYPTHIQPLWT
                     RDRGANTCTSCHNVSDHTDLSGTIGGDGRVVSYDRLMIGDPLLDSSGRPVITFDDGVP
                     MLEHQPALVNTAASEGEAMGLARKSRLMEILSGQSLMSDAAAKASHPNPPASAPDHSK
                     MLNAAEKRLLAEWIDTGGKYYNDPFDPTSGTRMVPTLDEDSFIASVYPILESTCAGCH
                     QARGSDNQPTGATSFTNNRYVLTGDPDGDWGVTMTMVNDVCHPPTSYLLARPSTIPHP
                     QAATTQTAAVLPANGTNYATIANWIAAGCMNP"
     gene            complement(5074..6048)
                     /locus_tag="VIN75_10760"
     CDS             complement(5074..6048)
                     /locus_tag="VIN75_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8974688.1"
                     /translation="MTSLHPPAIASAPPMSVPGSIGAGWYRREEAIMGTSIEVELLAR
                     DRAEAELAIGAVMAEMHRIDATMSPHKVTSELSILNAHAADNPITVSEGLFGLLARAL
                     AFSELSGGAFDITFASAGQLYDYRAGTGPDDQELARALPLIDWRHVLLDPADRSVRFA
                     RQGVRIDLGGFAKGHAVDNSIAILRRLGIRHAMVAAGGDSHVMGARGDRPWTVAIRDP
                     RQEGAVVALLPLEDVSISTSGDYERFYERDGVRCHHLLDPRTGRSPSAVRSVTIIADD
                     GLTTEAFSKTVFVLGVEQGLRFVEAHAGVDAVIVDAHGTLHYSAGLQG"
     gene            complement(6529..7242)
                     /locus_tag="VIN75_10765"
     CDS             complement(6529..7242)
                     /locus_tag="VIN75_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974689.1"
                     /translation="MTLFVILSLVLAAATLAILAWRGAPVAHVTDPATAATAVMRPTR
                     GLVVMLGALIAVVAIGGYAWIGSPRLLALTPDAPAGPGPAMDAQADVLQARAEAHPDD
                     AAGWMQAARMHAMLNHGAAAVADYRKVAALRPNDPNVLADLADMLAATGTGSLEGEPI
                     QLVDRALAIDPNHVKALALKGSYAMTQRDFRTALGAWNQALKVAQPGDPIAQFLRQQM
                     DAMREMAQRAAAGASAPAK"
     gene            complement(7239..7808)
                     /locus_tag="VIN75_10770"
     CDS             complement(7239..7808)
                     /locus_tag="VIN75_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY8974690.1"
                     /translation="MADVRRLLAALLVATAAAVASAAEAPASAPSAADAASEAASAPA
                     TVKFEAPTARLTAADPALEARMLAITGELRCLVCQNQTIADSHADLAVDLRQEVREML
                     QKGQTPEQIRKYMTDRYGDFVLYRPPLKASTAVLWLAPGVLLAIALLALVVVIRKRAR
                     LADDQFEPDPMLDDGTEAVDAAPRPGSLA"
     gene            complement(7801..8331)
                     /locus_tag="VIN75_10775"
     CDS             complement(7801..8331)
                     /locus_tag="VIN75_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY8974691.1"
                     /translation="MKALKFLIPLAIFAVLVAFFAKGLHLDPREVPSPLIGKPAPEFA
                     LTQLDKPGQTIRRADMLGKVWMLNVWASWCEACRDEHPYLVEFAKLKALPIYGLDYKD
                     QREPAQAWLAERGNPYDASLFDASGRVGIDFGVYGVPETFIIDKQGVIRFKQIGALTP
                     EVLQAQVLPLLRKLNG"
     gene            complement(8328..10376)
                     /locus_tag="VIN75_10780"
     CDS             complement(8328..10376)
                     /locus_tag="VIN75_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY8974692.1"
                     /translation="MIPELGQIALLIALAVALILGTLPLVGAARGRADWMALARPAAR
                     AHGLLVGVAFACLVSSFVRDDFSVLYVASNSNSALPLFYRVAGVWGGHEGSLLLWLTM
                     LSVWMLAVAQWSSKLPPPFVARVLGIMGLVSIGFILFMLTTSNPFDRLVPVPMDGNDL
                     NPLLQDPGMVSHPPMLYMGYVGFSVAFAFAVAALIEGKLDATWARWTRPWTTAAWSCL
                     TIGIMLGSAWAYYELGWGGWWFWDPVENASFMPWLAGTALIHSLAVTEKRGAFKSWTV
                     LLALLAFTLSLLGTFIVRSGVLSSVHAFATDPKRGLFILVFLGIVVGGALTLYAWRAP
                     KVGLGARFSLVSRESMLLGNNMLFVVTCASVLLGTMYPLLLDALGMGKISVGAPYFEA
                     VFLPLMVVVVVLLGIGPLARWKEASLPSLLHRLRFAALATVLGALGTSWVAGRLSLVA
                     MLGIAMTYWIAASIVVDIIERLRSSGGASLSIFERVRQWPRSLTGMWLAHLGVAVFAF
                     GVSMVRTYEVEEDLTMTVGASATVGGYEFRFADLHEVHGPNYEAIEADMPVTRGGSEV
                     TVLHPQKRVYRVQRTPTTEAAIQPGITRDLYVSLGEAVQGNNTWTLRVHVKPFVDWIW
                     GGCLLMALGGIVAVSDRRYRLRQRQAVAEDAAIAAPPTAFAQPVVPQAMAAPNLGRSE
                     "
     gene            complement(10373..10825)
                     /gene="ccmE"
                     /locus_tag="VIN75_10785"
     CDS             complement(10373..10825)
                     /gene="ccmE"
                     /locus_tag="VIN75_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY8974693.1"
                     /translation="MTPRQKRATIIVGILAAVGVATALVLNAFNSNLVFFYSPSQVAN
                     HEAPEGRPFRVGGMVTVGSVKREGVDVQFTVTDLVKSMQVHYRGPLPDLFKEGKGVVA
                     QGKLGPDGVFTAQEVLAKHDENYMPPEAAAALKKAAEHGGTMKTERHQ"
     gene            complement(10822..11034)
                     /gene="ccmD"
                     /locus_tag="VIN75_10790"
     CDS             complement(10822..11034)
                     /gene="ccmD"
                     /locus_tag="VIN75_10790"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY8974694.1"
                     /translation="MTTMQWHSLSEFIDMGGRGGFVWGAYGTMAVLMLVEPLLARRRH
                     RAARTAIAERMADEEAARAAAAGDRP"
     gene            complement(11031..11810)
                     /gene="ccmC"
                     /locus_tag="VIN75_10795"
     CDS             complement(11031..11810)
                     /gene="ccmC"
                     /locus_tag="VIN75_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEY8974695.1"
                     /translation="MNTSAATAAVRPRLTWFTFASPANFYRLAGVLGPLCGAVAAAFA
                     LAGLWMGFFVAPTDAVQGEVYRIIFIHVPAAWLSMVLYVAMAFWAAIGWFLNARMAFI
                     FARAIAPTGALFTLLALWTGSLWGRPTWGTWWVWDARLTSELILLFLYLGYMALVAAI
                     DDVRRADKAGALLAIVGVVNVPIIYFSVVWWNTLHQGSSLTMGKEQHMASSMLLAMLL
                     MTLACWAYAFAVVFARARAIVLEREGDNAWARALVAARTQG"
     gene            complement(11876..12547)
                     /gene="ccmB"
                     /locus_tag="VIN75_10800"
     CDS             complement(11876..12547)
                     /gene="ccmB"
                     /locus_tag="VIN75_10800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY8974696.1"
                     /translation="MNGAVFLAVYQRDLRLAWRRRTEALLPVAFFLVAASLFPLGVGP
                     DPQVLKLIAPGVVWVCALLAAMLSMPPLYAADHADGSLEQLLLAPGAGVAVAAAKAAG
                     HWTISGAPLVVGAPLLGLLLGLPAEELPVLFVSLSLGTPILSLLGGVGAALTLGLRSA
                     GMLIVLIVLPLAIPALIFGAGAAAAVDAGQSPSPHLSLLGALLIACLVGAPPATAAAL
                     RIAVE"
     gene            complement(12654..>13289)
                     /gene="ccmA"
                     /locus_tag="VIN75_10805"
     CDS             complement(12654..>13289)
                     /gene="ccmA"
                     /locus_tag="VIN75_10805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY8974697.1"
                     /translation="PQQLATRDRLLFSGLNETLARGELLWLRGGNGRGKTSLLRLVAG
                     LSPPEHGQVLWDGVPTRGNEHFDRALVYIAHANALKDDLTATESLAFLARVHGRDAGA
                     GALRAALARLGLAGRERTPVRSLSQGLRRRVALARLALESGPSLWVLDEPFDALDVAG
                     TATLHALLAAHRARGGSVLLTSHHLHLDAQTASAGFRVLDLDLLPREGAAA"
BASE COUNT         1897 a         4511 c         4946 g         1935 t
ORIGIN      
        1 ctcgaagtcg ttggacgggt acttgggctt gtgcacctcg cggcgctcga cctgcggcac
       61 gatcacctgg tcggaggtgt cggcggcggg cgcgtcggcg gcgcgcgcgc cgccgtgcgc
      121 cagcagcgcg agcgcggcgc ccgcgagcag cgtgagcgcg ccggcgcgca cggcgaggac
      181 ggtgggagac gggcgcttgg cggcgatggt tcggctcttg gcgatcatgg gcggcgcctc
      241 agaagaagaa ggagagtccg gcggtggccg cgcggtactc gagcgagcgc tggtcggaga
      301 cgaacgcggt gtagatcgcg tagtcggccc ggaccatgaa gcggtcggtg aggtggtagc
      361 gcgcgccgat gatggcgtcg gccaggcgcg cgttggtggg ctggaagttg accaggctct
      421 ggttcgggat gttgttgaac cggccgatgc cgatgcccag gaacggcgag aagcgctggt
      481 cggaccaggg ctcggcctgc aggttcacgt gccacagcgt cgtgcccgag aacacgccct
      541 gcacctggcc gtaggtcgac tcgaagctga gggtgtcgga gatgcggtac gagacccaga
      601 acttgatcat cgactcgcga tggaagcggc cgtagccggc gcccagctcc acgcggcggg
      661 cgaggtagtc gtcgtacttg acgtcgccga agtcgttggg caggccgccc tcggccagag
      721 tcgactgcag ctgctggcgc gtgacccagc cgaccttgcc ggtggcggtg cgcaccttgt
      781 accagtcggt gtgccgcagc tcgatcgaga tccactcgcc gcgggcggcg acgtagaaga
      841 ccgggtagcc gcggccggcg aaggtgtgca gctcgatgta cggatcggtg acctgcacgc
      901 gctcgcgctt gtcgccgccg ggcacgctgt cggccacggg caccgtgggc ggcgagccgg
      961 tggtcaccgg cgcggggatc tccacggtct gcgtcgagcc gggcaccggc acggtggccg
     1021 tgcgggggcg gtcgtcgact gcggccggcg cggaggcggc atcgcccggc acggcagcgg
     1081 cctgcggcgc ggaggcggcg tccgcaggcg cgggcgcgtc ggccgcgcgc gtgccggcgc
     1141 ctgcgagcgc gaaggccagc aacaccacgg cgcgttgcag cgcggtggac gaggtcgaaa
     1201 cggcgcgcac gccgcgaatc gcctgcaccc ctggcgctcc gcgccgcccc gccgagcggg
     1261 ggcattcgcc cttcgggcgg ccgggcgggc tcattgcgtg ttgcacgtac ccgtcgccgg
     1321 atccgccggg gtgaacatgc tgtagctgga cgtgctgaac tcgtcctggc ccgcgggcac
     1381 ggggtggctg atccagtagt cgatcagctt ggcgttcggg tcgtcctggc tcgtgaagat
     1441 gcggccgccg ccatgcagca cgttcagcac cagcggcttg tcgaacaggc ggctctggat
     1501 cgggctgccg aacacggtcg tgccttgcgc cgaatagaag ttcttgtaca tgtcggatgc
     1561 gcggatcgtg gtggccatgg cggagtccgt cgccgcggct gcgacatccg catccgcgat
     1621 cggcgtggcc gcgacgatca cgcgcaaggc gccgccggta cccgtgttgc tgtcatggca
     1681 gccggcgccc gcgcaggtgt tcgtcgaggt gacgccattc tgcgtgatgg gcagctgctt
     1741 gaggaagatc gggaagatgc acttctggaa ataaggaaag gcgagcgtgg cgccgccggt
     1801 cgtcgagggg ttctggacgt tgccggggtt gctcagggga tcgccgccac cgccgcagcc
     1861 ggcgagcagc ccgccggccg cgagggcgcc cagggccgcg cggcggaggc gcgcgcggcg
     1921 ccgggagggc gcgcgcgccg cggcgcgccc cgctcccatg tcgtcctgcg cgaagccgca
     1981 ggatgccacc ctcgttttct gcttctgcat cttcatggat tcatgcaacc tgccgcgatc
     2041 cagttggcga tggtcgcata gttcgtgccg ttggcgggca gtaccgcagc ggtctgggtg
     2101 gtcgccgcct gcgggtgcgg gatcgtcgac ggacgcgcca gcaggtagct ggtgggcgga
     2161 tggcagacgt cgttgaccat cgtcatcgtc acgccccagt caccgtccgg gtcgccggtc
     2221 agcacgtagc ggttgttcgt gaacgacgtg gcgccggtgg gctggttgtc ggagccgcgg
     2281 gcctggtggc agccggcgca ggtgctctcc aggatcgggt agaccgaggc gatgaacgag
     2341 tcctcgtcca gcgtgggcac catgcgcgtg ccgctggtcg ggtcgaacgg gtcgttgtag
     2401 tacttgccgc cggtgtcgat ccattcggcc agcaggcgct tctcggcggc gttgagcatc
     2461 ttggagtgat ccggcgcgct ggccggcggg ttcgggtgcg aggccttggc ggcggcgtcg
     2521 gacatcagcg actggcccga gaggatctcc atcaggcggc tcttgcgcgc caggcccatc
     2581 gcctcgcctt cgctggcggc ggtgttcacg agcgcgggct ggtgctccag catcggcacg
     2641 ccgtcgtcga aggtgatcac cgggcggccc gaggagtcga gcagcggatc gccgatcatc
     2701 aggcggtcgt acgacaccac gcggccgtcg ccgccgatgg tgccgctcag gtccgtgtgg
     2761 tcggacacgt tgtggcacga cgtgcaggtg ttggcgccgc ggtcacgcgt ccacagcggc
     2821 tggatgtgcg tcgggtagtt gatgacgccg ttgacgggca ccggggtgac caggtcgtcc
     2881 gacgggttcg ggttgccggt gtagcgcagc gcgacggccg ggcgggcggt gacgccggcc
     2941 ttggtcgtgt cggcccagat gtcggtggac accatgtcgg gcttgagctg cgcggcggtc
     3001 gggtcgaggc gcacgcgcgt gtcggccatc gtctcgccgg actggtgcgc gttggccatg
     3061 gcggccagga tcgcggcggg caccgtgttg acggtcgcgc ccgagttgat cggggcgccg
     3121 cggcgcgggc tgtggcagcc gtcgcaggtg cggcgttcgc ccggacgtac ctggatccag
     3181 ttggtgtgcg tctggaaggc gcggccctgc gagtcgacca cggccagcgc gatcggggtg
     3241 tccgccggga cgttcagctt gaacgagccg tccggttcga tcggcgcgta gccgagtacc
     3301 tgctgcgcct cgaagtcggt ctcgcccagc gagctgcgca tcttcatcat gctggtcggc
     3361 ggcgccacgg cgcgcaccgc gcggatgaag cgggccggcg cgcagttgta ggcggcgttg
     3421 gccgggtcct tcatcttgtt gatgtcggcc acgctggcgc gggtgtcgtt gacgtcgatc
     3481 ggcgcggtct gcgggatcag cgggcccgtg cagccggcgt tggcgtcggc ggcggtcagc
     3541 atgccttcgc ccatgcggcc gaggccgtcg gtgtcgtaga cgctgcgcac gtcgagcgtg
     3601 cccatcttct gcgacgcgag cgtctcgtcc agcggcgcgg cggcgacggt ggtcggctcc
     3661 gtgcgcgcct ggatggcgac ggcgtcgacg tacatgaagc cggccggcgg cgtggccacc
     3721 ggcagcatcg actggttggc cgggttgaac atgtagatgg cgtacggcgg cgggacgttg
     3781 tcctgcaccg ggtcggcctg gctctcggcc agcgggcggt tcatgtcccc cagcttggcg
     3841 agctcgtcgg cggtcagcgt cgagcagggg acgaccgtgc cgttcttggt gacctcgcac
     3901 gggcggtacg acaccagcac gcggtcggtg ccgtcccaca gcgggtacgg gccggtgaag
     3961 cggccgtaca tcgagacgcc gcggtcgatg ttgaccgtct gcgtggtggc ctgctgctgg
     4021 ccgccggcgg cggcgaccga cttcttggcc ggcgtgttct gctcggagta gttggccgcg
     4081 tcgatcagca tcagcgcgcc gccctcatgc gtgcgctgca gcggcatcag gtccgacgtg
     4141 aggaagccgg cgtacttgcc cttgggatcc atgtcgcgcg gatgcaggaa gctgttgccg
     4201 tcgctgtgcg cgccgtacag cacgaacatg tcggtgccgt ccgggttgac cgtgaacacc
     4261 ttgaagtggt tgcggccgcc gacgtggtcc cagcgcgaga acatgatctg gccgttcggg
     4321 cggatcgtcg ggttgcggtc gtggctctgg ttgaacgaga tctgcgtgat gttgctgccg
     4381 tccatcgcca tcgtgtgcag gttgaacacc cgctcgcgct cgtactcgtc gagcgcgtaa
     4441 tagctgtggc cgagcgcctg gttggccttg gactcggtct ggcggttcga cgagaagacg
     4501 atgccctggc cggccggcag gtagaccggg tggatgtcgt cgtcggcggt ggacgacgtg
     4561 atgcggcgga acgtgccgcc ggtcaggccg cccgtggtca tgtcgtattc ccagacgttc
     4621 cagtggccgg tgcaggccgc ggcgccgccg atctgcacgg cgttgtcggc cgggcagcgc
     4681 atcgagaaca ggatcttctt gccgtcccac gacacctcgg gcgtggcggc gtcgcccttg
     4741 cccatcgtga actgcgcggt gatgttgtgc tcgggcgcgc tgggcgagga cttctcgcgg
     4801 atcatcaggt cgccgcccgg cgcggtgggc gcgccgttgg tggggttcag gccgatcgtg
     4861 ttgacgcgct tggcgtacac gatgggcacg tcgccctgca ccgtgacggt gttggactcc
     4921 gtcttggagc cgccgccgca ggccgcgagc gacagcacgc cggccccggc cagcgcgctc
     4981 gcgccaccca gcagggtgcg tgtggtgaac gagatgggca gtttcatgag ttgcctcttt
     5041 ccttgcagtt cttgtcttgt cagcctgggt tcttcagcct tgcaggcccg ccgagtagtg
     5101 cagcgtgccg tgcgcatcca cgatcaccgc gtcgacgccc gcgtgggcct cgacgaagcg
     5161 caatccttgt tcgacgccca gcacgaacac cgtcttggag aaggcttcgg tggtcaggcc
     5221 gtcgtcggcg atgatggtca cgctgcgcac ggcgctcggc gagcggccgg tgcgcgggtc
     5281 gagcaggtgg tggcagcgga cgccgtcgcg ctcgtagaag cgctcgtagt cgccggaggt
     5341 ggagatcgag acgtcctcca gcggcagcag cgcgacgacg gcgccttcct ggcgcgggtc
     5401 gcggatggcc accgtccagg ggcggtcgcc gcgcgcgccc atgacgtggc tgtcgccgcc
     5461 ggcggccacc atcgcgtggc ggatgcccag gcggcgcagg atggcgatcg agttgtcgac
     5521 cgcgtggccc ttggcgaagc cgcccaggtc gatgcgcacg ccctggcgcg cgaagcgcac
     5581 gctgcggtcg gccgggtcca gcagcacgtg gcgccagtcg atcagcggca gcgcgcgcgc
     5641 gagctcctgg tcgtcggggc cggtgccggc gcggtagtcg tagagctggc cggcgctggc
     5701 gaaggtgatg tcgaacgcgc cgcccgacag ctcggagaac gccagcgcgc gggcgagcag
     5761 gccgaacagg ccctcgctca cggtgatcgg gttgtcggcc gcgtgcgcat tcaggatcga
     5821 cagctccgac gtgaccttgt gcgggctcat cgtcgcgtcg atgcggtgca tctcggccat
     5881 gacggcgccg atggccagct cggcctccgc gcggtcgcgg gccagcagct cgacctcgat
     5941 cgacgtgccc atgatggctt cctcgcggcg ataccagccc gcgccgatcg atccgggaac
     6001 ggacatcggc ggcgcgctgg cgatggcggg cgggtggagg ctggtcatgg acgcttcctc
     6061 ggacctggcg tggtccgggg aatcctgagt cggtcgattc gttgtcgttg ggtcggtggt
     6121 gccgccccgg tcggacggcg atggcgggca cgccgtttgc acggttttcc gctttgggcg
     6181 catcttcgca gtgaagcgct tcgaccaagc gtgaaaaagt tccgtatttt tcgtcacgct
     6241 gtatcgcgtg gcgggtaatc ggtaactgaa cgttctgtta ggagggtccc ggcctcagtt
     6301 gtgtacaaaa gcaacgtcga ggacgcggtt tcgcgcattt gtgcacaaat cccatttagt
     6361 tggcggatcg tgcgcgcagg gggcctgttc cgccggctgg gccggctcgc cgaggtggat
     6421 cctgcgactg cgcttcgctc cgcgccggat gacggcgtcg gggacttcgg gcgatgctcg
     6481 caagccaggt gtcatcctgc gcgcagtcgc aggatccacc tcgaccgact acttcgccgg
     6541 cgccgacgcc ccggcggccg cgcgctgcgc catctcgcgc atcgcgtcca tctgctggcg
     6601 caggaactgc gcgatcgggt cgcccggctg ggccaccttc agcgcctggt tccacgcgcc
     6661 aagtgccgtg cggaagtcgc gctgcgtcat cgcgtagctg cccttgagcg ccagcgcctt
     6721 cacgtggttc ggatcgatcg ccagcgcgcg atcgacgagc tggatcggct cgccctcgag
     6781 gctgccggtg ccggtggcgg ccagcatgtc ggccaggtcg gccagcacgt tcgggtcgtt
     6841 ggggcgcagc gcggcgacct tgcggtagtc cgccaccgcc gcggcgccgt ggttgagcat
     6901 ggcgtgcatg cgggcggcct gcatccagcc cgcggcatcg tcgggatgcg cctcggcgcg
     6961 cgcctgcagc acgtcggcct gcgcgtccat cgcgggaccg gggccggccg gcgcgtcggg
     7021 cgtgagcgcc agcaacctgg gcgagccgat ccaggcgtag ccgccgatgg ccacgacggc
     7081 gatgagcgcg cccagcatca cgaccagccc gcgcgtgggc cgcatcacgg ccgtggcagc
     7141 ggtggccggg tcggtgacgt gcgcgaccgg cgcgccgcgc caggccagga tggccagcgt
     7201 ggccgcggcc agcaccaggg acaggatcac gaacagcgtc atgcgaggct tccaggacgg
     7261 ggcgccgcgt cgacggcctc ggtgccgtcg tcgagcatcg ggtcgggttc gaactggtcg
     7321 tcggccagcc gcgcgcgctt gcggatcacg acgaccagcg ccagcagcgc gatcgccagc
     7381 aacacgcccg gcgccagcca cagcaccgcg gtgctggcct tgagcggtgg ccggtacagc
     7441 acgaagtcgc cgtagcggtc ggtcatgtac ttgcggatct gctcgggcgt ctggcccttc
     7501 tgcagcatct cgcgaacttc ctggcgcagg tcgacggcca ggtcggcgtg cgagtcggcg
     7561 atcgtctggt tctggcagac caggcagcgc agctcgccgg tgatggccag catgcgcgcc
     7621 tcgagcgccg ggtcggcggc ggtcagccgg gccgtgggcg cctcgaactt caccgtggcc
     7681 ggagcggacg cggcctcgga ggcagcgtcg gcggccgacg gcgcggacgc cggcgcctcg
     7741 gcggcggagg cgaccgccgc ggccgtggcg accagcagcg cggcgagcag gcggcggacg
     7801 tcagccattg agcttcctca gcagcggcag cacctgcgcc tgcagcacct cgggcgtcag
     7861 cgcgccgatc tgcttgaagc ggatcacgcc ctgcttgtcg atgatgaagg tctcgggcac
     7921 gccgtagacg ccgaagtcga tgcccacgcg gccgctggcg tcgaacagcg aggcgtcgta
     7981 cgggttcccg cgctcggcca gccaggcctg ggccggctcg cgctggtcct tgtagtcgag
     8041 cccgtagatc ggcagcgcct tgagcttggc gaactcgacg agatacgggt gctcgtcgcg
     8101 gcaggcctcg caccacgagg cccacacgtt gagcatccac accttgccca gcatgtcggc
     8161 gcggcggatg gtctgcccgg gcttgtccag ctgcgtcagg gcgaactcgg gcgcgggctt
     8221 gccgatgagc ggcgacggca cctcgcgcgg gtccaggtgc aggcccttcg cgaagaaggc
     8281 gaccagcacc gcgaagatcg cgagcgggat caggaacttc agcgccttca ttccgagcgt
     8341 cccaggttcg gcgcggccat ggcctgcggc accacgggct gcgcgaacgc cgtgggcggc
     8401 gcggcgatgg cggcgtcctc ggccaccgcc tggcgctggc gcaggcggta gcggcgatcg
     8461 ctgacggcca cgatgccgcc gagcgccatc agcaggcagc cgccccagat ccagtcgacg
     8521 aagggcttga cgtgcacgcg cagcgtccac gtgttgttgc cctgaacggc ctcgccgagc
     8581 gacacgtaca ggtcgcgcgt gatgccgggc tggatcgcgg cctcggtggt gggcgtgcgc
     8641 tggacgcggt agacgcgctt ctgcggatgc agcaccgtca cctcgctgcc gccacgcgtg
     8701 acgggcatgt ccgcctcgat ggcctcgtag ttggggccgt gcacctcgtg caggtcggcg
     8761 aaccggaact cgtagccgcc gacggtggcg ctggcgccga ccgtcattgt caggtcttcc
     8821 tcgacctcgt aggtgcgcac catgctcacg ccgaacgcga acacggccac gcccaggtgc
     8881 gcgagccaca tgcccgtcag cgagcgcggc cactggcgca cgcgctcgaa gatcgacagg
     8941 ctggccccgc cggacgagcg caggcgctcg atgatgtcga cgacgatcga cgccgcgatc
     9001 cagtacgtca tcgcgatgcc cagcatcgcc acgagcgaca gccggcccgc gacccacgac
     9061 gtgccgagtg cgcccagcac cgtggcgagc gccgcgaagc gcaggcgatg cagcagcgac
     9121 ggcagcgagg cttccttcca gcgcgccagc ggcccgatgc ccagcagcac gacgaccacc
     9181 accatcagcg gcaggaagac ggcctcgaag tacggcgcgc ccaccgagat cttgcccatg
     9241 cccagcgcat cgagcagcag cgggtacatc gtgcccagca gcaccgaggc gcaggtcacg
     9301 acgaacagca tgttgttgcc cagcagcatg gactcgcgcg agaccagcga gaagcgcgcg
     9361 cccaggccca ccttcggcgc gcgccaggcg taaagcgtca gtgcgccgcc cacgacgatg
     9421 cccaggaaca cgaggatgaa caggccgcgc ttcggatcgg tggcgaacgc gtgcaccgac
     9481 gacagcacgc ccgagcgcac gatgaaggtg cccagcagcg agagcgtgaa ggccagcagc
     9541 gcgagcagca cggtccacga cttgaaggcg ccgcgcttct cggtgacggc cagcgagtgg
     9601 atcagcgccg tgccggccag ccacggcatg aacgacgcgt tctccaccgg gtcccagaac
     9661 caccagccgc cccagcccag ctcgtagtag gcccaggcgc tgcccagcat gatgccgatc
     9721 gtcaggcacg accaggcggc cgtcgtccac gggcgcgtcc agcgcgccca ggtggcgtcg
     9781 agcttgcctt cgatcagggc ggcgaccgcg aacgcgaagg ccaccgagaa gccgacgtag
     9841 cccatgtaga gcatcggcgg gtggctcacc atgcccggat cctgcagcag cgggttcagg
     9901 tcgttgccgt ccatgggcac cggcacgagg cggtcgaacg ggttggacgt ggtcagcatg
     9961 aacaggatga agccgatcga caccaatccc atgatgccca gcacgcgcgc cacgaacggc
    10021 ggcggcagct tgctgctcca ctgcgcgacg gccagcatcc acacgctcag catcgtcagc
    10081 cacagcagca gcgagccctc gtggccgccc cacacgccgg ccacgcggta gaacagcggc
    10141 agcgcgctgt tggagttgct ggcgacgtac agcaccgaga agtcgtcgcg cacgaagctc
    10201 gacacgaggc acgcgaacgc cacgcccacc agcagcccat gcgcgcgcgc cgcgggacgc
    10261 gcgagcgcca tccagtcggc ccggccgcgc gcggccccga cgagcggcag cgtgcccagg
    10321 atcagcgcca ccgccagcgc gatcagcagc gcgatctggc ccagttccgg gatcattgat
    10381 gtctctccgt cttcatcgtg ccgccatgct cggcggcctt cttcagcgcc gccgccgcct
    10441 cgggcggcat gtagttctcg tcgtgcttgg ccagcacttc ctgcgccgtg aagacgccgt
    10501 ccgggccgag cttgccctgc gcgacgacgc ccttgccttc cttgaacagg tcgggcagcg
    10561 gtccgcgata gtgcacctgc atcgacttga cgaggtcggt gacggtgaac tgcacgtcga
    10621 cgccctcgcg cttgacgctg cccaccgtca ccatgccgcc gacgcggaac gggcgaccct
    10681 cgggcgcctc gtggttggcc acctgcgacg gcgaatagaa gaacaccagg ttggaattga
    10741 acgcgttgag caccagcgcc gtggccacgc ccacggccgc gaggatgccg acgatgatcg
    10801 tcgcgcgttt ctggcgcggg gtcatggccg gtctcccgcc gcggccgcgc gcgcggcttc
    10861 ctcgtcggcc atgcgctcgg cgatcgccgt gcgcgccgcg cgatggcgcc ggcgcgcgag
    10921 cagcggctcg acgagcatca ggacggccat cgtgccgtag gcgccccaca cgaagccgcc
    10981 gcggccgccc atgtcgatga attcggaaag cgagtgccac tgcatcgtgg tcatccttga
    11041 gtccttgcgg cgaccagggc ccgcgcccag gcgttgtcgc cctcgcgctc cagcacgatc
    11101 gcccgtgccc gggcgaacac caccgcgaac gcgtaggccc agcaggcgag cgtcatcagc
    11161 agcatcgcca ggagcatgct cgaggccatg tgctgctcct tgcccatcgt caggctcgag
    11221 ccctggtgca gcgtgttcca ccagacgacc gagaaataga tgatgggcac gttgaccacc
    11281 cccacgatgg ccagcagcgc gccggccttg tcggcgcggc gcacgtcgtc gatcgcggcc
    11341 accagcgcca tgtagccgag gtagaggaac agcaggatca gctcggacgt gagccgcgcg
    11401 tcccacaccc accacgtgcc ccaggtggga cggccccaca gcgagcccgt ccacagcgcc
    11461 agcagcgtga acagcgcgcc ggtgggcgcg atcgcacggg cgaagatgaa ggccatgcgc
    11521 gcgttcagga accagccgat cgccgcccag aaggccatcg ccacgtacag caccatcgac
    11581 agccaggccg cgggcacgtg gatgaagatg atgcggtaga cctcgccctg gaccgcgtcg
    11641 gtgggcgcga cgaagaaccc catccacagg ccggccaggg cgaacgcggc cgcgacggcg
    11701 ccgcacagtg gccccagcac gcccgccagc cggtagaaat tggccggcga cgcgaacgtg
    11761 aaccaggtca ggcgggggcg gacggcggcg gtggcggcgg acgtgttcac gtgtgcattg
    11821 tcccgttgtc ggcgcgagtc gtgttgcgag atgtcatggc acgggccccc aggggtcatt
    11881 ccaccgcgat gcgcagcgcc gcggcggtgg ccggcggcgc ccccaccagg caggcgatca
    11941 gcagcgcgcc caggagcgac aggtgcggcg acggcgactg cccggcatcg accgccgccg
    12001 ccgcgccggc gccgaagatc agcgccggga tggccagcgg cagcacgatc agcacgatca
    12061 gcatgccggc cgagcgcagg cccagcgtca gcgccgcgcc caccccgccc agcaggctca
    12121 ggatcggcgt ccccagggaa agagacacga acagcaccgg aagttcctcg gccggcaggc
    12181 ccagcagcag gcccagcagc ggggcgccga cgaccagcgg cgcgccgctg atcgtccagt
    12241 gcccggcggc cttggccgcg gccaccgcga cgcccgcgcc gggcgccagc agcagctgct
    12301 cgagcgagcc gtcggcgtgg tcggcggcgt acagcggcgg catcgacagc atcgccgcca
    12361 gcagcgcgca cacccacacg acgcccggcg cgatcagttt gagcacctgc ggatcgggtc
    12421 cgacgcccag cgggaacagg ctggccgcga cgaggaagaa cgccaccggc agcagcgcct
    12481 cggtgcggcg gcgccaggcc aggcgcaggt cgcgctggta gacggccagg aagacggcgc
    12541 cgttcaatgg agcccctccg gcgccgggtg gggcgctgcg tccgccgagg ggaccgcgga
    12601 ccggcttggg gcggcccggc gcccggcctg cgggcgggtt gacgggcgcg tgctcatgcg
    12661 gcggcgccct cgcgcggcag caggtcgagg tcgagcacgc gaaagcccgc gctcgcggtc
    12721 tgcgcgtcca ggtgcaggtg gtggctggtc agcagcacgc tgccgccgcg ggcgcggtgc
    12781 gcggcgagca gcgcgtggag cgtggccgtg ccggcgacgt cgagcgcgtc gaagggctcg
    12841 tcgagcaccc acagcgaggg cccggactcg agcgccagcc gggcgagcgc gacgcgccgc
    12901 cgcaggccct gcgacaggct gcgcacgggg gtgcgctcgc gcccggccag gcccaggcgt
    12961 gcaagcgcgg cgcgcagcgc gcccgcgccg gcgtcccgcc cgtgcacgcg ggcgaggaag
    13021 gccagcgact cggtggccgt caggtcgtcc ttgagcgcat tggcgtgggc gatgtagacc
    13081 agcgcgcggt cgaaatgctc gttgccccgc gtgggcacgc catcccagag cacctggccg
    13141 tgctcgggcg gcgacagccc ggccaccagc cgcagcaggc tcgtcttgcc gcgcccgttg
    13201 cccccgcgca gccacagcag ctcgccacgc gcgagcgtct cgttcaggcc cgagaacagc
    13261 aggcggtcgc gcgtggcgag ctgctgtgg
//
LOCUS       DATCRO010000400         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_960841, whole genome shotgun sequence.
ACCESSION   DATCRO010000400 DATCRO010000000
VERSION     DATCRO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960841"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..760)
                     /gene="sctR"
                     /locus_tag="VIN75_10810"
     CDS             complement(101..760)
                     /gene="sctR"
                     /locus_tag="VIN75_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEY8974698.1"
                     /translation="MNGSLPDPLTLVALIVAFGLAPFAALMVTCYTKLVIVFGLLRTA
                     LGLQQTPPNMVLNGIAIILSVYIMAPIGMDVADALRNHSFGQKGEAVSDIMAVADAAK
                     QPLKDFLQKHTHERERRFFMDSATSVWPKARAQALRSDDFIVLVPSFTLSELTRAFQI
                     GFIIYMVFVVVDLIVATILLALGMSMISPTTISLPFKLLLFVMLDGWARLIHGLVLSY
                     R"
     gene            complement(792..1901)
                     /gene="sctQ"
                     /locus_tag="VIN75_10815"
     CDS             complement(792..1901)
                     /gene="sctQ"
                     /locus_tag="VIN75_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HEY8974699.1"
                     /translation="MNLADPPSDRLTPSPDRAPVAELGNAPALPRLDAALARAFNRLH
                     DRRAAPITLNVGGADRVLQWVYDDSGTLPPLDTHRFRIGPHDGRIGVDQPALAALLGE
                     ARPDRIPRPLRAVLLADALAACADALARATRLHFEWQPGDATGPAFDASAALRFRVDG
                     TGWSGALQFDAPGALDALAPGLVAAAPSRRSTTNDALAALRLPLRFHIGRTRLRLAEV
                     RGIVPGDIVAIEEWRAAGAAVVVESTPGGQAGPRFTALAEGSRITIQQTREQTMNRPA
                     DPPPAATAPQAPELAVDRLDAIEMTLRFEVGELAISLGELRNVRAGHVFELGQPLNRG
                     PVRIVAHGNLLGKGTLVAVGDRLGVRVSEFAPGDL"
     gene            2110..>2965
                     /locus_tag="VIN75_10820"
     CDS             2110..>2965
                     /locus_tag="VIN75_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY8974700.1"
                     /translation="MYMTTKGRNAAPAAFGGDGPRRGGRNNDLVLAALLVLIVVLFVL
                     PLPTALLDLLIATNLAASLVLLIVAMYVPSASSLSTFPSLLLFTTLFRLALNIASTKL
                     ILLHANAGHIIDTFGKLIVGNNVVVGGVVFLIIAIVQFVVIAKGSERVAEVAARFALD
                     AMPGKQMSIDADVRAGAMSSAEAQRRRQALEQESALHGAMDGAMKFVKGDAVASIIVA
                     LVNILAGLAIGVLMKDMSVGDAVHRYAILTVGDGMVSQIPSLLVSIAAGVVITRVDAS
                     GGDPQLANQ"
BASE COUNT          432 a          986 c         1108 g          439 t
ORIGIN      
        1 gtcctagacg aggccgggct gcaggcgctg ctggcggcgc cgtcggatgc cgtcgacgag
       61 gcggcgggtc cggatgcctc ccggcaggcc ggcacataga tcagcgatac gacagcacca
      121 gcccgtggat cagccgtgcc cagccgtcca gcatcacgaa cagcagcagc ttgaacggca
      181 gcgagatcgt cgtcggcgag atcatcgaca tgcccagcgc gagcaggatg gtcgcgacga
      241 tcaggtcgac gacgacgaac accatgtaga tgatgaagcc gatctggaac gcgcgtgtga
      301 gctcgctgag cgtgaagctg ggcacgagca cgatgaagtc gtccgagcgc agcgcctgcg
      361 cgcgcgcctt gggccacacg ctggtggccg agtccatgaa gaagcggcgc tcgcgctcgt
      421 gcgtgtgctt ctgcaggaag tccttcagcg gctgcttggc ggcgtccgcg acggccatga
      481 tgtcggacac cgcctcgccc ttctggccga agctgtggtt gcgcagcgcg tcggccacgt
      541 ccatgccgat cggcgccatg atgtagaccg acaggatgat ggcgatgccg ttgagcacca
      601 tgttgggcgg cgtctgctgc agcccgagcg cggtgcgcag caggccgaag acgatcacca
      661 gcttggtgta gcaggtcacc atcagcgcgg cgaacggcgc gaggccgaac gcgacgatca
      721 gggcgacgag ggtcagcgga tcgggcaggc tgccgttcac ggcgccggcc tccgcggcga
      781 gggcgcggcc gtcacaggtc gcccggcgcg aattcgctga cgcgcacgcc cagccggtcg
      841 ccgacggcca ccagcgtgcc cttgcccagc aggttgccgt gcgccacgat gcgaacgggc
      901 ccgcggttga gcggctggcc gagctcgaac acgtggccgg cgcgcacgtt gcgcagctcg
      961 ccgaggctga tcgcgagttc gccgacctcg aagcgcagcg tcatctcgat cgcgtcgagg
     1021 cggtcgacgg cgagctcggg ggcctgcggc gcggtggcgg cgggcggcgg gtccgcggga
     1081 cggttcatgg tctgttccct cgtttgctgg atcgtgatgc gcgagccttc ggccagcgcg
     1141 gtgaagcggg gtccggcctg gccgccgggc gtggactcga cgacgacggc ggcgccggcc
     1201 gcgcgccatt cctcgatggc gacgatgtca cccggcacga tgccgcgcac ctccgccagg
     1261 cgcaggcgcg tgcggccgat gtgaaagcgc agcggcaggc gcagcgcggc cagcgcgtcg
     1321 ttcgtggtgg accgcctgga cggtgccgcc gcgacgaggc cgggcgcgag cgcgtcgagg
     1381 gcgcccggcg cgtcgaactg cagcgcgccg gaccagcccg tgccgtcgac gcgaaagcgc
     1441 agcgcggcgc tggcgtcgaa cgccggcccg gtcgcgtcgc cgggttgcca ttcgaagtgg
     1501 agccgtgtcg cgcgcgccag tgcgtccgcg cacgccgcga gcgcgtcggc aagcagcacg
     1561 gcgcgcagcg ggcgcgggat gcggtcgggc cgcgcctcgc ccaacagcgc ggccagggcg
     1621 ggctggtcga cgccgatgcg gccgtcgtgc gggccgatgc ggaaccgatg cgtgtccagc
     1681 ggcggcagcg tgccgctgtc gtcgtatacc cactgcagta cccgatccgc cccccccacg
     1741 ttcaaggtga tgggtgcggc gcggcggtcg tgcaggcggt tgaaggcgcg cgcgagcgcg
     1801 gcgtccaggc ggggcagcgc gggcgcgttc ccgagctcgg cgaccggggc gcgatcgggt
     1861 gatggcgtca gtcggtcgga gggcgggtcg gcgaggttca cggcgtgttc tggcgggttg
     1921 tcgtgcgacg ggctggtcgc gctgccgtga gggtagccaa gcagggtggc tgctggaaag
     1981 tacgaagaat cgtaggtatg cgcgcggcga cggcgcgcat gcccgtcagg catgactacg
     2041 agctctcgtt gttgccgggc tcgagtgccg aacctagagt ggcctcaagc catccgcgca
     2101 taccggtaca tgtatatgac gacaaaggga cgcaacgccg ctccggccgc attcggcggc
     2161 gacgggccac gccgtggcgg ccgcaacaac gacctcgtgc tcgcggcgct gctcgtgctc
     2221 atcgtcgtgc tgttcgtgct gccgctgccg acggcgctgc tggacctcct gatcgccacc
     2281 aacctcgcgg ccagcctcgt gctgctcatc gtggcgatgt acgtgccgtc ggcctcgtcg
     2341 ctgtcgacct ttccttcgct gctgctgttc accacgctgt tccgactcgc gctcaacatc
     2401 gcgtcgacca agctgatcct gctgcacgcc aacgccggcc acatcatcga taccttcggc
     2461 aagctcatcg tcggcaacaa cgtcgtcgtt ggcggcgtcg tgttcctgat catcgcgatc
     2521 gtgcagttcg tcgtgatcgc caagggctcg gagcgcgtgg ccgaggtcgc ggcgcgcttc
     2581 gcgctggacg ccatgcctgg aaagcagatg agcatcgacg ccgacgtgcg ggccggcgcg
     2641 atgtcgtcgg ccgaggcgca gcgccggcgg caagcgctcg agcaggagag cgcgctgcac
     2701 ggcgcgatgg acggcgcgat gaagttcgtc aagggcgacg ccgtggccag catcatcgtc
     2761 gcgctcgtca acatcctcgc cggcctggcg atcggcgtgc tgatgaagga catgagcgtg
     2821 ggcgacgcgg tgcaccgcta cgcgatcctc acggtgggcg acggcatggt ttcgcagatc
     2881 ccgtcgctgc tggtgtcgat tgccgcgggc gtcgtgatca cgcgcgtcga cgcgtccggg
     2941 ggcgacccgc agctggccaa ccaga
//
LOCUS       DATCRO010000401         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_724337, whole genome shotgun sequence.
ACCESSION   DATCRO010000401 DATCRO010000000
VERSION     DATCRO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724337"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..826)
                     /locus_tag="VIN75_10825"
     CDS             complement(368..826)
                     /locus_tag="VIN75_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974701.1"
                     /translation="MTTAFAFPSFPAFQGSEKFSPEAIQASLRPVMEQAQAWADLGKK
                     HFDETRAATEAALKSFTGVKDPQAAIDVLKANAQQGLVLAGEQLRASAELGVSQFHAT
                     VDAAAAKLPQADAFAPVAKGLKAGVDAAHNSLSSVLDQVAPVAKKTSRRA"
     gene            complement(932..>2187)
                     /locus_tag="VIN75_10830"
     CDS             complement(932..>2187)
                     /locus_tag="VIN75_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEY8974702.1"
                     /translation="TDVDTSGYQWQGVDWHAPGGLSISRSDYTGQFSNPGGGSAFIET
                     VLDFDSLANGSVSNGDFIADDGSTPRVQLAASGQSQSFAPGDDAALHAGVAWPATRWV
                     DHHDGTVTDTLTGLTWLRDAGCLAPAAWAGALDAVNHLAAGACGLSDGSAAGQWRLPN
                     QWELESVVDESAAAPAIGAAAPFVNVANAAYWTSTSYYGGQAGSSSAWAIRLGDGRYI
                     NDGIANAKTAALGVWAVKGDGSGGAVRLQATGMYVPYAAGDDGSLRTGVALPFPRLRD
                     NGDGTLTDTLTGLVWLKRANCLGGDWSSAVAAVRRLASGQCGLTDGSGAGAWRMPNRK
                     EMASLADRALNNQADFFGTSWTSTEAGIAPSAAPFDQFIAFQYYWTSSTDATDTTEAW
                     TVFSCDFGIYDTAKSATGYTLAVRD"
BASE COUNT          300 a          715 c          830 g          342 t
ORIGIN      
        1 gcactgagcg gctacctgtt ctgatccgcc gcctgcgcgg gcccgcgccc gcgcttcacg
       61 cgcatgcctg ctacatgaac cgcggcgtcg cgatcctgaa cttccgaggc gctcgcgaag
      121 gcgcgccgcc cgggtgcacg ctcgatgcag ttcctcggct tcacgacacg tttccggctg
      181 cggggtcgcc acggggtcag gtcttgaaag ttgcgcggcg acttctccga tatcgccgaa
      241 tcttcgtcgc gcaactttca agacctgacc ccgtggcgac cgaccccgtg gcgacccgag
      301 acgcggcacc agaatgagaa acgccctcgc cgccgaagcg gggagggcgt cctggggagc
      361 gcgaggatca ggcgcggcgc gaggtcttct tggcgaccgg ggcgacctgg tcgaggaccg
      421 acgacagcga gttgtgcgcg gcgtccacgc cggccttcag gcccttggcg accggcgcga
      481 acgcgtcggc ttgcggcagc ttggcggcgg cggcatccac ggtggcgtgg aactgcgaga
      541 cgcccagttc ggcggaggcg cgcagctgct cgccggccag cacgaggcct tgttgcgcgt
      601 tggccttcag gacgtcgatg gcggcctgcg ggtccttcac gccggtgaac gacttcagcg
      661 cggcttcggt ggcggcgcgg gtctcgtcga agtgcttctt gccgaggtcg gcccaggctt
      721 gcgcctgttc catgaccggg cgcaggctgg cctggatcgc ttcgggcgag aacttctcgc
      781 taccttggaa agcggggaag gacgggaagg cgaaagcggt ggtcatggtg ggtctccggt
      841 tgaatggtgc agtgcagcaa ttgtattgca accatgctgc agtgcaacat aaaacttagg
      901 ggatgccctc cagaagtccg tccgggccgc ttcagtcgcg cacggccagc gtgtagcccg
      961 tggcgctctt ggcggtgtcg tagatgccga agtcgcagct gaacaccgtc caggcctcgg
     1021 tggtgtcggt ggcatcggtg ctggacgtcc agtagtactg gaaggcgatg aactggtcga
     1081 agggggcggc cgagggggcg atgccggcct cggtgctggt ccaggaagtg ccgaagaagt
     1141 cggcctggtt gttcagggcg cggtcggcca ggctggccat ctccttgcga ttgggcatgc
     1201 gccaggcgcc ggcgcccgag ccgtcggtca ggccgcactg gcccgaggcg aggcggcgca
     1261 cggccgcgac ggcgctgctc cagtcgccgc ccaggcagtt ggcgcgcttg agccagacca
     1321 ggccggtgag ggtgtcggtg agcgtgccgt cgccgttgtc gcgcaggcgc gggaagggca
     1381 acgcgacgcc cgtgcgcagg ctgccgtcgt cgcccgccgc gtagggcacg tacatgccgg
     1441 tggcctgcag tctgacggcg ccgccgctgc cgtcgccctt gaccgcccac acgcccaggg
     1501 cggccgtctt cgcattggcg atgccgtcgt tgatgtagcg gccgtcgccc agccggatgg
     1561 cccaggcgct ggacgaaccg gcctggccgc cgtagtagct ggtggatgtc cagtacgccg
     1621 cgttggcgac gttgacgaac ggcgccgcgg cgccgatggc gggcgcggcc gccgactcgt
     1681 cgaccacgct ctccagctcc cactggttgg gcaggcgcca ctggccggcg gccgagccgt
     1741 cggacaggcc gcaggcgcct gcggccaggt ggttcacggc gtcgagggcg ccggcccacg
     1801 ccgccggcgc gaggcagccg gcgtcgcgca gccaggtcag gccggtgagg gtgtcggtga
     1861 cggtgccgtc gtggtggtcg acccagcgcg tcgcaggcca ggcgacgccg gcatgcaggg
     1921 cggcgtcgtc acctggcgcg aagctctgcg actggccgct ggcggccagt tgcacgcgcg
     1981 gcgtgctgcc gtcgtcggcg atgaagtcgc cgttcgatac ggagccgttg gccagcgaat
     2041 cgaagtcgag cacggtctcg atgaacgcgg agccgccgcc cgggttcgag aactggccgg
     2101 tgtagtccga gcgcgagatc gacaggccgc cgggggcgtg ccagtcgacg ccctgccact
     2161 ggtagccgga ggtgtcgacg tccgtgt
//
LOCUS       DATCRO010000402        14378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_94094, whole genome shotgun sequence.
ACCESSION   DATCRO010000402 DATCRO010000000
VERSION     DATCRO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94094"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VIN75_10835"
     CDS             <1..1017
                     /locus_tag="VIN75_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY8974703.1"
                     /translation="DAGAPDGNAPAWIEADELAWLDTVELPPFDAGDAALASMTAPAS
                     PLAGEAAAAAASTGTSTAAPLADCVVWLERNYRFGLDSPIGRLSLAIRRGDVQAALDA
                     LPADESAAASFHEDAGDALASSTVERLARRFGAYLDALRDALAAPVPDPLPLFDALNR
                     FRILCATRSGSRGAEHVNALVAAHVRHAARVPLAVGAHWFTGRPIMVTRNDYALGLFN
                     GDIGIALPDAQGELLVHFPDATAAGGFRAIAPVRLPRHETAFAMTVHKSQGSEFDAVL
                     VMLPQQRSRVLTRELLYTAITRARRGVALVADPPVLEQAIATPTVRHSGLLARLAEEE
                     GQRG"
     gene            1703..2620
                     /locus_tag="VIN75_10840"
     CDS             1703..2620
                     /locus_tag="VIN75_10840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8974704.1"
                     /translation="MLIPVCNGCPFFRAAVDSVLGQSFEDFECLILDDGSVDDTGRIA
                     DEFARSDARVRVIHRENRGLVPSLNELVAAARGEFIARMDADDLCTPWRFARQVDYLR
                     QHPDTVCVGSSYWMMDEAGRIITQIMPPRDDAAIQGQALRGHSTISHPSAMLRTAALR
                     RLGGYRAAYFPAEDLDLWLRLGELGTLANLPEPLIHYRVHAASISARSVQGRQRDAAR
                     RACVDAWARRRVSDGQFEATREWRADGTATSRLRFALQYGWMAYGSGFRQTAMVYGLK
                     AIRALPTRQDGWRLLAVALLRPPRKPIPL"
     gene            2730..3530
                     /locus_tag="VIN75_10845"
     CDS             2730..3530
                     /locus_tag="VIN75_10845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8974705.1"
                     /translation="MRLSVITPTLNSARFIRACVDNVAAQGSCVLEHIVVDGGSTDGT
                     LPLVEELRRSHPRLRLLPGPDRGQSDAMNKGSAAALGEAIGVLNVDDAYEPHACAEAL
                     QQLQSMKRPGMVVGDCRVVGEHGELRFWNRPTNLRLEALLLGWSYAQFPCNPSAYFYH
                     RAVHELVGGYDVDDHYAMDFDFILNCARQVQMRYVPRHWGNFRLAPGCKTFDDDRGRE
                     RVAALIERHRARLGWLGAARMRLLEARIDFHRWRKAADAPKRAMGTPS"
     gene            3527..4894
                     /locus_tag="VIN75_10850"
     CDS             3527..4894
                     /locus_tag="VIN75_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8974706.1"
                     /translation="MSTAVPQEAANPVPPTGLAQRVLRGSGWTLAGTVSSQFLRFASN
                     LVLTRLLFPEAFGLMAIAQSILTAAHLLSDVGLTQSIVRSERGHEPRYQDTIWTLNIA
                     KSLLIGLLMGVAGPIAAHVYAQPALARIVPAMGLPAVIGAFASTKVALINRRLEIGRL
                     TLLELGAQACGIATMVAWALVDRSPWALVAGNWASTLATVAGSHLLIAGRGNRLAWDR
                     AIVREVLSFGGWVLMSSGVTFLLGEGSNLLTASLVGTKSVAFIGLSTTLALVAWNAIQ
                     QVSGRVLFPAYSEVWRQRARDFPRVVERARRLQLLGACAVAVVLVLFGDRIVQALYDP
                     RYRPVGALLQIQAVGTIFAFASSSYANVLWAMGRAGLNTVILAAQAFMLIGLLTLGNT
                     LGGVLGLMTGTSFIGLAIYPVNAFIFARFGLFQPKVDLPVFLLGACAAGWVWLFGAWR
                     VVSFH"
     gene            4955..5407
                     /locus_tag="VIN75_10855"
     CDS             4955..5407
                     /locus_tag="VIN75_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974707.1"
                     /translation="MHIALESPDQPDVIALIADLDAYQDTLYPPESRHALDLASLKRD
                     NVRFFVARDAAGRAVGCCAVVLGADHGEIKRMYVRPDCRGQGLASGLLRAVEAASAQA
                     RCPRLMLETGPYQPEAIGLYASFGYARRGPYAGYRDDPLSVFMEKALA"
     gene            5574..6194
                     /locus_tag="VIN75_10860"
     CDS             5574..6194
                     /locus_tag="VIN75_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8974708.1"
                     /translation="MEHLEKFRDDELSVTISELLVATADSSDPLLDDSLTDLLHAMRT
                     RMQMDVVFVSEFIEGRRMFRYVDRSPGSPDIDAGGSNPAEESVCQRVVEGRVPELVRD
                     LSKVDMAQAGLPELPFQIGAHLSTPIVLRSRRIYGTLCCFSAAPRPDLVQADLDKLKL
                     CATLVARKVDLNQSRGIVEPPPDWKLEPMDTYQSPAWQKASPRAGR"
     gene            complement(6228..6725)
                     /locus_tag="VIN75_10865"
     CDS             complement(6228..6725)
                     /locus_tag="VIN75_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974709.1"
                     /translation="MNTIADPDRPLQPGTMVLEMATSRGAAGRALVFAAAVALVVGGC
                     IAALARFSPGAVAAIVAGVAIYAWLMCSEPQVAYVEYFRLDASGVSFVHTPGQRGRVS
                     RHAWSQIEDVSIGAGASGQESPGLVLSLRPGAAIRGEVVLPILGDAERLRARDLARAW
                     LASRA"
     gene            complement(6748..8034)
                     /locus_tag="VIN75_10870"
     CDS             complement(6748..8034)
                     /locus_tag="VIN75_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8974710.1"
                     /translation="MTSRATKTIMAAALAACAGAGVQAATTVVTADRMLDVLSGRMVE
                     RPAIVVVDGRIHAVLTQGAAAIPKDASHIDLPGQTLLPGLIDMHVHLAADARIADNHA
                     LDYTDSFWVAVGVANAKAMLEAGFTTVRNVGSPDYADVGIKQAVEGGYVPGPRIVPAT
                     YALGTTGGHCDDTEGLPPSFSMVPTPSVADSPDGFRALVRRVHKYGAEIIKICATGGV
                     LSRTDAAGAQQMSYEEIKAVTDEAHRLGLKVAAHAHGTSGINDALRAGVDTIEHASLA
                     DAESFRLAKEHGAWFSMDIYDDDYILAEGARNGMYEASLAKERAIGLKQRQTFRAAYE
                     AGVKMIFGTDNGAVFPAGQNALQFAKMVEWGMPPLEAIRAATRNAAQALAREGDVGAI
                     EAGRYGDFVAVKGDPLQDVRLLEHPSAVIKGGERVK"
     gene            complement(8074..9795)
                     /locus_tag="VIN75_10875"
     CDS             complement(8074..9795)
                     /locus_tag="VIN75_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8974711.1"
                     /translation="MNLKLKLPLAFCGVLAALMGAALIGIHSLNDSVDVYEVDVGAQH
                     ARERAIADVLVTFKTQVQEWKDTLLRGKDPAKLDKHWGAFAKREADVDDKAKALLASL
                     PPGESRTLVEQFSAAHVKMGADYRGGFAAFKDAGFDPSAGDAAVAGMDREPARLLAEA
                     GKKIAADSAAASADAAVKAHRASLVSIVLMLVAGAAGIGVGVLFSRKITGPVDDAVTV
                     AEAIAGGDLTRQVRVMGRDEIAQLLKALATMQGSLAGVVSNVRSNSESVATASSQISQ
                     GANDLSARTEEQASALQQAAASMEQLGSTVRQNAENAQSANQLALGASAVARKGGDVV
                     GEVVETMKGINDSSKRIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGE
                     VRSLAQRSSDAAKEIKALISASVERVEHGTALVDRAGETMTEIVTSIARVTDIMGEIS
                     AASTEQSSGVGQIGDAIAQMDQATQQNAALVEESAAAAESLKDQAHQLVQVVSVFKLE
                     GRATDDFRVAPTSFAAPRPVAAPKPAAPVRKPAPMAARPAPVSAKVSARVAEPELAST
                     ADDWESF"
     gene            complement(10066..11370)
                     /locus_tag="VIN75_10880"
     CDS             complement(10066..11370)
                     /locus_tag="VIN75_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY8974712.1"
                     /translation="MDRLEIAMRDVEDARIGALRRMKWIAAGLLVAALAGLALASWQG
                     GEGGWGWLQSFCEASAVGAIADWFAVVALFRRPLGLPLPHTAILPRSKERVADGLAHF
                     VRDHFLEPTSLVLRLAELDPARRLGDWLADPARVHDWVAEGRRWALKAVGLFDDERMR
                     RATLELVVAQARRWNSAATAADVLALLTRGGRHHELLDAGLEKIGGFLAEDEVKQRVS
                     DLMVRHARKEWPKVVGMVELVTPVARMADGLASKLSASVLGELREVLSQPDHPVRQRY
                     DKWLAEFIERLRGDESLVAAFDRVKERAITDPAVLDYAGGVWNDIKRLLQADLADERS
                     ALAEHVAQAMRDVGDRLREDASLRASLNEHLLSAAGTIAANLRSGVTEHIARTIKDWD
                     DRRLVEQLELSVGRELQFIRINGTVVGGIAGLALHALRLLLH"
     gene            11466..13715
                     /locus_tag="VIN75_10885"
     CDS             11466..13715
                     /locus_tag="VIN75_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8974713.1"
                     /translation="MSSEPANPPTSIPAAAPRAFKLPNARHASRVPEDVTPADIARIQ
                     QAAYQLPQVVKPADYIDEHAPHFEQHLDGLGLILLQKAVSGGRPSRWIAPAHVAAALR
                     HLDVVGNGPQVRAQMQGRTAELEALGITREAGRFDRFLQHDLRLPASGVQVRVAWRQP
                     IADAPVWDFYRTADIDALLAAIGDPPPYPDADELVEHVERLLARAESGHPEHVGSLVQ
                     RLQAEAARPQAFADLKASLVRAQDRWERHVAELNALAGLNSELAFQGYPDSFELARRL
                     QRTVTLYVGPPNSGKTHAAFEQLAAAHDGCYLAPLRLLALEGRDRLVARGVPTSLLTG
                     EENDPAEGARVVSSTIEMVNTSRPIDVAVIDEAQMLFDGSRGWAWTQAIVGVPANEVI
                     IICSAYAAPAIENLLKLCGENCKVRSFERKQHVELLPGPVPISALKKGDAVVAFSRKE
                     VLMLRDQVAADGHPVSVIYGALPPEVRRREAERFAHGHAHILVATDAIGMGLNLPIRR
                     VLFSTLKKFDGIGDRLLDESEVHQIAGRAGRYGLHEEGFAGVLREAEPAALRTLKDLL
                     PRQPRAPRDFKAFVAPNWWHVDTIATRLGLARLRAVLDVFVDQLRLDDAHFAVAELEQ
                     MLELAEQLDHNAGKLTLKQRFIYAQPAVDTRTETQVQAFPEWAWNHANAGQAGTPWFL
                     SEVDEHSRLDNMEKALRSCTLWLWLDLRFPGTYGHLEEVIDLRTRLNEGIERHLKGKK
                     PLWQQRGRR"
     gene            complement(13961..>14378)
                     /locus_tag="VIN75_10890"
     CDS             complement(13961..>14378)
                     /locus_tag="VIN75_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8974714.1"
                     /translation="AMDNATLSGATYDAFRAHPLLRGVDEVLQRQWPPNQVVEEHTHP
                     FDADASRAWLVHWLGSGLRAVEQRLATEPGTGRFCHGDQPTIADICLASLFMVTRVLK
                     IDVAGIPVSTRILAECERLPAFAAAAPALQLGAPAL"
BASE COUNT         1954 a         5282 c         4997 g         2145 t
ORIGIN      
        1 gatgctggcg caccagacgg caatgcgccc gcctggatcg aggccgacga actcgcatgg
       61 ctcgacaccg tcgagctgcc gccgttcgac gcaggcgatg cggcacttgc atcgatgacg
      121 gcgcccgcat cgccattggc cggcgaagcg gcagccgcag ccgcgtccac cggtacgtcc
      181 accgcggcac cgctcgcgga ctgcgtggtg tggctagaac gcaattaccg cttcggtctc
      241 gattcgccga tcgggcggct gtcgctcgcg atccgtcgcg gcgacgtgca ggcggcactc
      301 gatgcgctgc ccgccgacga gtcggccgcc gcgtcgtttc acgaggatgc gggcgacgcg
      361 ctggcgtcgt cgacggtcga gcggctcgca cggcgcttcg gcgcctacct cgacgcgttg
      421 cgcgacgcgc tggccgcgcc ggtgcccgat ccgctgccgc tgttcgacgc gctcaaccgg
      481 ttccggatcc tgtgcgcgac ccgcagcggc tcgcgcggcg cggaacacgt caatgcgctg
      541 gtggccgcgc acgtgcggca tgcggcgcgc gtgccgctcg cggtcggcgc gcactggttc
      601 accgggcggc cgatcatggt cacacgcaac gactacgcgc tggggctgtt caacggcgac
      661 atcggcatcg cgctgcccga cgcgcagggc gagctgctcg tccacttccc cgacgccacg
      721 gccgccggcg gcttccgcgc catcgcgccg gtgcgcctgc cgcgccacga gacggccttc
      781 gcgatgaccg tccacaagtc ccagggctcg gagttcgacg cggtgctggt gatgctgccg
      841 cagcagcgca gccgcgtgct gacgcgcgag ctgctctaca ccgccatcac gcgcgcccgc
      901 cgcggcgtcg cgctcgtggc ggacccgccc gtgctcgaac aggccatcgc cacgccgacg
      961 gtgcggcact cgggcctgct ggcgcggctg gccgaggagg aaggccagcg aggttgagcc
     1021 gtccgctcga cgcgccctgg tccgagctgg ctcgaccgcg cgatccgcgc gcgtcatgca
     1081 cgccttcgct cgcatcatgg gtgagcagcg ttgaccgcaa ttcctgtctc cgacgccgcg
     1141 gacgcgctct tgtcgagggc cgcgacgctc agcgtcgttt cagtgcggat tcagctcgcg
     1201 tcgcgatgcc tgtgggcccg ctgaatcggc gttgaacggg gcgcgctgcg cacgagcatt
     1261 cgccggccga acagtcgcgt cgccgggcgc gcacgaaccc atgcggaggc ggcatgccga
     1321 ctgcgcgggg tcgtcgttgc gttcggcacc cgatctcgac gcgtgacgag ctggccgccc
     1381 cggggcgact cgcgcgctgc cggctgaagt cacgctccac acggcgcatg cggcggtctg
     1441 tccgcatgcg tcggcgcatg gatcgcggca gccacgcagc gcggccgttc gcgcgccctg
     1501 aaagcctggt gatagtccgc agacaacttg gcgacaccga cgcggcgttg cgattgcccg
     1561 gcgagccccg caccgggcaa gcggtgcgac gttcgaatcc aacggcatcg agggtctcca
     1621 attgacagct gcgacaccac atcttccatc ggcccgaacg gggacatccg ccccgggtgg
     1681 cgatggagca cccaccgtct ccgtgctgat tcctgtctgc aacggctgcc cgttcttccg
     1741 tgccgccgtc gacagcgtgc tcggccagag cttcgaggac ttcgagtgcc tgatcctgga
     1801 cgacggctcg gtcgacgaca ccggcagaat cgccgacgag ttcgcccgca gtgatgcgcg
     1861 cgtgcgcgtc atccaccgcg agaaccgcgg cctggtaccg agcctgaacg agctcgtcgc
     1921 ggccgcacgc ggcgagttca tcgcacgcat ggacgccgac gatctctgca cgccctggcg
     1981 cttcgcgcgt caggtcgact acctgcgcca gcatcccgac accgtctgcg tgggcagcag
     2041 ctactggatg atggacgagg cgggccgaat catcacgcag atcatgccgc cacgcgacga
     2101 cgccgccatc cagggccagg ccctgcgcgg gcactcgacg atctcgcatc catcggccat
     2161 gctgcgcacc gccgcgttgc ggcgcctggg cggctaccgg gccgcctatt tcccggccga
     2221 ggatctcgat ctctggctgc gcctgggcga gctcggaacg ctcgccaacc tgcctgagcc
     2281 gctgatccac taccgcgtgc acgcggcatc gatcagtgcc cgctcggtgc agggacgtca
     2341 gcgcgacgcg gcacggcggg cctgcgtcga cgcctgggcg cgccgccggg tcagcgacgg
     2401 ccagttcgag gcgacgcgcg aatggcgcgc cgacgggacc gcgacctcgc gcctgcgctt
     2461 cgcgctgcag tacggctgga tggcctacgg atccgggttc cgacagaccg cgatggtgta
     2521 cgggctgaaa gcgatccgcg cgctgccgac ccggcaggac ggctggcgcc tcctggccgt
     2581 cgcgctgttg cggccgccac gcaagccgat ccctctgtga cgtcatcgga ccggggaatg
     2641 gtcgcttctg actttccgcg acctgaagcc gtgcgcgcgc cggcatgccg gttgccgcct
     2701 cttgccgcgc cgcgggcgcc cgacaccgca tgagactctc ggtcatcacg cccaccctga
     2761 actcggcacg cttcatccgt gcctgcgtgg acaacgtcgc cgcccagggc agttgcgtgc
     2821 tcgaacacat cgtggtcgac ggcggttcca cggacggcac gctgccgctg gtcgaggagt
     2881 tgcgtcgctc gcacccccgc ctgcggctgc tgccggggcc ggatcgaggt cagtccgacg
     2941 cgatgaacaa gggctccgcc gcggcgctcg gcgaggcgat cggtgtcctc aacgtggacg
     3001 acgcctacga gccccacgcc tgcgccgagg cgctgcagca gttgcagtcc atgaagcggc
     3061 caggcatggt ggtcggcgat tgccgtgtcg tcggcgagca cggcgagctt cgcttctgga
     3121 accggccgac gaacctgcgc ctggaggccc tgctcctggg ctggtcctac gcgcagttcc
     3181 cctgcaatcc gagcgcctac ttctaccacc gcgccgtcca cgagcttgtc ggcggctacg
     3241 acgtcgacga ccactacgcg atggacttcg acttcatcct gaactgcgcg cggcaggtcc
     3301 agatgcgcta tgtcccgcgc cactggggca atttccgcct ggcacccggc tgcaagacgt
     3361 tcgacgacga ccgcgggcgc gagcgcgtcg ccgcgctgat cgagcggcac cgcgcacggc
     3421 tcggctggct gggcgcggcg cgcatgcgcc tgctcgaggc ccggatcgat ttccatcgct
     3481 ggcgcaaggc ggcggacgct cccaagcgcg ccatgggaac accgtcttga gcaccgccgt
     3541 cccgcaggaa gccgcgaacc ccgtgccgcc gacgggcctg gcgcagcgcg tcctgcgcgg
     3601 cagcggctgg accctggccg gcaccgtcag cagccagttc ctgcgcttcg ccagcaacct
     3661 ggtgctgacg cggctgctgt tcccggaggc cttcggcctg atggcgatcg cacagtcgat
     3721 cctcacggcg gcgcacctgc tgtcggacgt cggactgacc cagagcatcg tgcgcagcga
     3781 gcgcggacac gagccgcgct accaggacac catctggacg ctcaacatcg ccaagtcgct
     3841 gctgatcgga ctgctgatgg gcgtcgccgg gcccatcgcc gcgcacgtct acgcgcagcc
     3901 ggcgctggcc aggatcgtcc ccgccatggg cctgcccgcc gtcatcggcg cattcgcgtc
     3961 gaccaaggtg gcactgatca accggcggct ggagatcggc cggctgacct tgctggaact
     4021 gggtgcgcag gcctgcggca tcgcgacgat ggtcgcctgg gccctggtcg atcgttcgcc
     4081 ctgggccctg gtcgcgggca attgggcgag cacgctggcg acggtggccg gcagccacct
     4141 gctgatcgcc ggccgcggca accggctcgc gtgggatcgc gcgatcgtgc gcgaggtgct
     4201 ctcgttcggc ggctgggtgc tgatgtcgtc cggcgtgacc ttcctgctcg gcgaaggcag
     4261 caacctgctg accgcctcgc tggtgggcac gaagtcggtc gccttcatcg gcctgtcgac
     4321 gacgcttgcg ctggtcgcat ggaacgccat ccagcaggtc agcgggcgcg tgctgtttcc
     4381 ggcctactcg gaggtctggc gccagcgcgc ccgcgacttc ccgcgcgtcg tcgaacgcgc
     4441 gcgccggttg caactgctcg gcgcctgcgc ggtcgcggtc gtcctcgtgc tgttcggcga
     4501 ccggatcgtc caggccctct acgacccgcg ctaccggccc gtcggcgcgc tgctgcagat
     4561 ccaggccgtg ggcacgatct tcgcgttcgc gagctcgtcg tacgcgaacg tgctgtgggc
     4621 catgggacgg gccggcctga acacggtcat cctggcggcg caggccttca tgctgatcgg
     4681 cctgctcacg ctgggcaaca cgttgggcgg ggtgctgggc ctgatgacgg gcacgtcctt
     4741 catcgggctg gcgatctatc cggtcaatgc gttcatcttc gcccgcttcg ggctcttcca
     4801 gcccaaggtc gacctgccgg tgttcctgct cggcgcgtgc gccgccggct gggtctggct
     4861 gttcggcgcg tggcgcgtgg tgtcgttcca ctgagcgccg cgagcggcgc cgtccgctac
     4921 gatggcgacg atgctccacc ctgccctcgc accgatgcac atcgccctgg agtcccccga
     4981 ccagcccgac gtgatcgccc tgatcgccga cctggacgcc taccaggaca cgctgtaccc
     5041 gcccgaaagc cggcacgcgc tcgacctggc ctcgctcaag cgcgacaacg tgcgcttctt
     5101 cgtcgcgcgt gacgccgcgg gccgtgcggt cggctgctgt gcggtggtgc tcggggccga
     5161 ccacggcgag atcaagcgca tgtacgtgcg gcccgactgc cggggccagg gcctcgcgag
     5221 cggcctgctg cgcgcggtcg aggccgcgtc cgcccaggcg cgctgcccgc gcctcatgct
     5281 cgagaccggg ccgtaccagc ccgaggcgat cggcctgtac gcgtcgttcg gctatgcgcg
     5341 gcgcgggccg tacgccggct atcgcgacga cccgctgagc gtgttcatgg agaaggccct
     5401 ggcctgaccg tgcgccgggc gccagcggcg ccgggcctgc ccaggacagg ctgcggctcc
     5461 gcgcaagttt tcgttacctg ttcctcgcgc ggtccgggac atgcgttcag taaactcggc
     5521 gcagggaaga accgatccga cgggacgcct cagatcccgc tccgcacccg cccatggaac
     5581 atctcgaaaa attccgcgac gacgaactca gcgtcaccat ctcggaactg ctcgtggcca
     5641 ccgccgactc cagcgacccg ctgctcgacg actccctgac cgacctgctg cacgcgatgc
     5701 gcacgcgcat gcagatggac gtcgtgttcg tctccgagtt catcgagggc cggcgcatgt
     5761 tccgctacgt cgaccgttcg cccggctccc cggacatcga cgccggcgga tcgaacccgg
     5821 ccgaggagtc ggtgtgccag cgcgtggtcg agggccgcgt gcccgaactg gtgcgggacc
     5881 tgtccaaggt ggacatggcc caggccggcc tgcccgagct gcccttccag atcggcgccc
     5941 acctgagcac gcccatcgtg ctcaggagcc ggcgcatcta cggcacgctg tgctgcttca
     6001 gcgcggcgcc gcgtcccgac ctggtgcagg ccgacctgga caagctcaag ctgtgcgcca
     6061 cgctggtcgc gcgcaaggtc gacctgaacc agtcgcgcgg catcgtcgag ccgccgcccg
     6121 actggaagct cgagccgatg gacacctacc agtcgcccgc ctggcagaag gcgtcgccgc
     6181 gcgcgggacg ctgagcgtcg cacccgggcg cgcaggccgt cgcgcggtca ggcccggctc
     6241 gcgagccagg cccgcgcgag gtcccgcgca cgcaggcgtt ccgcatcccc caggatcggc
     6301 agcacgacct cgccgcggat cgccgcgccg ggccgcagcg acagcacgag cccaggcgac
     6361 tcctgcccgc tcgcgccggc gccgatcgac acgtcctcga tctggctcca cgcatggcgc
     6421 gagacgcgcc cgcgctgccc cggcgtgtgc acgaaactca cgccgctcgc gtcgagcctg
     6481 aagtactcga cgtaggccac ctgcggttcc gagcacatga gccacgcgta gatcgccacg
     6541 cccgcgacga tggccgcgac ggcgcccggc gagaagcggg ccagcgcggc gatgcagcca
     6601 ccgacgacca gcgccacggc cgcggcgaac acgagcgcgc ggccggcggc gccgcgcgag
     6661 gtcgccatct ccaggaccat cgtcccgggc tggagcggcc ggtccggatc ggcgatggtg
     6721 ttcatgcgtg ccccggtacc tcgcgggtca cttcacccgc tcgccgccct tgatgaccgc
     6781 gctcgggtgc tccagcagcc gcacgtcctg gagcggatcg cccttgacgg ccacgaagtc
     6841 gccgtagcgg ccggcctcga tcgcgccgac gtcgccctcg cgcgcgagcg cctgcgccgc
     6901 gttgcgcgtg gcggcgcgga tggcctccag cggcggcatg ccccactcca ccatcttggc
     6961 gaactgcagc gcgttctggc cggcggggaa gacggcgccg ttgtcggtgc cgaagatcat
     7021 cttcacgccg gcctcgtagg ccgcccggaa ggtctggcgc tgcttcaggc cgatggcgcg
     7081 ctccttggcg agcgaggcct cgtacatgcc gttcctggcg ccttcggcca ggatgtagtc
     7141 gtcgtcgtag atgtccatcg agaaccaggc gccgtgctcc ttggcgaggc ggaacgactc
     7201 ggcgtcggcc aggctcgcgt gctcgatggt gtccacgccc gcccggagcg cgtcgttgat
     7261 gccggacgtg ccgtgcgcat gcgcggccac cttcaggccg agacggtgcg cctcgtcggt
     7321 gacggccttg atttcctcgt acgacatctg ctgcgcgccg gccgcgtccg tgcgcgacag
     7381 cacgcccccc gtggcgcaga tcttgatgat ctcggccccg tacttgtgca cgcggcgcac
     7441 cagcgcgcgg aacccgtcgg ggctgtcggc gaccgacggc gtgggcacca tggagaagct
     7501 cggcggcagg ccctcggtgt cgtcgcagtg gccgcccgtg gttccgagcg cgtacgtcgc
     7561 cggcacgatg cgcgggcccg gcacgtaacc gccttcgacg gcctgcttga tgccgacgtc
     7621 ggcgtagtcg ggggagccga cgttgcgcac ggtggtgaag ccggcctcga gcatcgcctt
     7681 ggcgttggcc acgcccacgg cgacccagaa gctgtcggtg tagtcgaggg cgtggttgtc
     7741 ggcgatgcgg gcgtcggcgg ccaggtggac gtgcatgtcg atcaggcccg gcagcagcgt
     7801 ctggcccggc aggtcgatgt gcgacgcgtc cttggggatc gccgccgcgc cctgcgtgag
     7861 cacggcgtgg atgcggccgt cgacgaccac gatcgcgggc cgttccacca tccggccgga
     7921 cagcacgtcc agcatgcggt cggcggtcac gacggtggtc gccgcctgca cgccggcgcc
     7981 ggcgcaggcc gccagcgcgg ccgccatgat ggtcttcgtt gccctgctcg tcatgtccgc
     8041 tccttgtcga gcggacactt tacccgcagg ggctcagaac gattcccagt cgtcggcggt
     8101 cgacgccagc tcgggttcag ccacgcgcgc cgacaccttg gcgctcaccg gcgcgggccg
     8161 ggcagccatc ggcgcgggct tgcgcacggg cgccgcgggc ttgggagccg cgacgggccg
     8221 gggcgcggcg aacgaggtcg gcgcgacgcg gaaatcgtcc gtggcccggc cttcgagctt
     8281 gaacaccgac accacctgca ccagctggtg cgcctggtcc ttcaggctct cggcggccgc
     8341 ggcgctctcc tcgaccagcg cggcgttctg ctgcgtggcc tggtccatct gcgcgatggc
     8401 gtcgccgatc tggccgacgc ccgagctctg ctccgtgctg gcggcgctga tctcgcccat
     8461 gatgtcggtg acgcgcgcga tcgaggtcac gatctcggtc atggtctcgc cggcgcggtc
     8521 caccagcgcg gtgccgtgct cgacgcgttc gacgctggcc gagatgagcg ccttgatctc
     8581 cttggcggcg tccgacgagc gttgcgccag ggagcgcacc tcgcccgcca ccacggcgaa
     8641 gccgcggccc tgctcgccgg cgcgcgcggc ctcgaccgcc gcgttcagcg caagaatgtt
     8701 ggtctggaac gcgatgccgt cgatcacgcc gatgatgtcg acgatgcgct tggagctgtc
     8761 gttgatgccc ttcatggtct cgacgacctc gcccaccacg tcgccgccct tgcgcgcgac
     8821 ggcgctggcg cccagcgcga gctggttggc cgactgcgcg ttctcggcat tctgtcgcac
     8881 cgtggagccg agctgttcca tcgacgcggc ggcctgctgc agcgcggagg cctgttcctc
     8941 ggtgcgcgcg ctcaggtcgt tggcgccctg cgagatctgc gacgacgccg tcgccacgct
     9001 ctcggagttg ctgcgcacgt tgctcaccac gccggccagg ctgccctgca tcgtcgccag
     9061 ggccttgagc agctgcgcga tctcgtcgcg gcccatcacc cgcacctgcc tggtcaggtc
     9121 gccgccggcg atcgcctcgg ccacggtcac ggcgtcgtcc accggaccgg tgatcttgcg
     9181 gctgaacagc acgcccacgc cgatgcccgc agcgcctgcc accagcatca gcacgatgct
     9241 gacgagcgac gcgcgatgcg ccttcaccgc ggcgtcggcc gaagcggcgg cgctgtcggc
     9301 cgcgatcttc ttgccggcct cggccagcag gcgcgcgggt tcgcggtcca tgccggcgac
     9361 ggcggcgtcg ccggcggagg ggtcgaagcc ggcgtccttg aacgcggcga agcccccccg
     9421 gtagtcggcg cccatcttca cgtgcgcggc gctgaactgt tccaccagcg tgcggctctc
     9481 gcccggaggc agcgacgcca gcagcgcctt ggccttgtcg tcgacatcgg cctcgcgctt
     9541 ggcgaacgcg ccccagtgct tgtcgagctt ggccgggtcc ttgccgcgca gcagcgtgtc
     9601 cttccattcc tgcacctgcg tcttgaaggt gacgagcacg tctgcgatgg cacgctcgcg
     9661 cgcgtgctgc gcaccgacgt ccacttcata gacatcgacg gaatcgttga gactgtggat
     9721 gccgatcagc gccgcgccca tgagcgcggc gaggacaccg cagaaggcca gcggcagttt
     9781 gagcttgagg ttcatgaggg actcgggccg aaggggggtg gggtcgccgt gcgatcgcga
     9841 ggcagagcct cgtgtcgacg gctgttcttg ccgggcgcgc catcctgtgc ggaggaacac
     9901 gggacgcacc ttcgtgtcct gttttcggcc gccgccccgc caacttgagt cgggagatag
     9961 cctcactggc ctgaaatcgc tttctgaggc agccaccatt cggtgcccgc caaggatcgc
    10021 atgtgctctt tccccggcac gtccgggcga gccgggtgcg tgccgctagt gcagcagcag
    10081 gcgcagcgcg tgcagcgcca ggccggcgat gccacccacg acggtgccgt tgatgcggat
    10141 gaactgcagc tcgcgcccga cgctcagctc cagctgctcg acgagccgcc ggtcgtccca
    10201 gtccttgatg gtccgggcga tgtgctcggt cacgccggag cgcaggttgg cggcgatggt
    10261 gccggccgcc gacagcaggt gctcgttgag cgacgcgcgc aggctggcgt cctcgcgcag
    10321 ccggtcgccg acgtcgcgca tcgcctgtgc cacgtgctcg gcgagcgccg aacgctcgtc
    10381 ggcgaggtcc gcctgcagca gccgcttgat gtcgttccag acgccgccgg cgtagtccag
    10441 caccgcgggg tcggtgatcg cgcgctcctt gacgcggtcg aacgcggcga cgagcgactc
    10501 gtcgccgcgc agcctttcga tgaactcggc cagccacttg tcgtagcgct ggcgcacggg
    10561 gtggtccggc tgcgacagca cctcgcgcag ttcgcccagc accgaggcgc tcagcttgga
    10621 cgcgaggccg tcggccatgc gcgccacggg cgtcaccagc tccaccatgc ccaccacctt
    10681 gggccactcc ttgcgcgcgt ggcgcaccat caggtcggag acgcgctgct tgacctcgtc
    10741 ctcggccagg aagccgccga tcttctccag gcccgcgtcc agcagctcgt ggtggcggcc
    10801 gccccgggtc agcagcgcca gcacgtcggc cgcggtggcc gcgctgttcc agcggcgcgc
    10861 ctgcgccacg accagctcca gcgtcgcgcg ccgcatgcgc tcgtcgtcga acagtccgac
    10921 cgccttgagc gcccagcgcc ggccctcggc gacccagtcg tgcacgcggg ccgggtcggc
    10981 cagccagtcg cccaggcgcc gcgccgggtc gagctctgcc aggcgcagca cgagcgaggt
    11041 cggctcgagg aaatggtcgc gcacgaaatg cgccaggccg tcggccacgc gctccttgct
    11101 gcgcggcagg atggccgtgt gcggcaaggg caggcccagc ggccggcgga acagggccac
    11161 cacggcgaac cagtcggcga tcgcgccgac ggccgacgcc tcgcagaagg attgcagcca
    11221 gccccagccg ccctctccgc cctgccacga cgcgagcgcg agccccgcca gcgcggcgac
    11281 cagcaggccc gcggcgatcc atttcatgcg ccgcaacgcc ccgatgcggg cgtcctcgac
    11341 gtcgcgcatc gcgatttcca ggcgatccat cgtcggcagt gtgccctgca tcgctcgtcg
    11401 agcggtcata ctgttggttt tcctccgaac gacgcacccc gcgcgagacg cgcccgtgca
    11461 cccgaatgag ctccgaaccc gcgaacccac cgacctccat ccccgccgcc gcgccgcgcg
    11521 cgttcaagtt gcccaacgcc cgccacgcca gccgcgtgcc cgaggacgtc accccggcgg
    11581 acatcgcccg catccagcag gcggcgtacc agctgcccca ggtcgtcaag ccggccgact
    11641 acatcgacga gcatgcgccg cacttcgagc agcacctgga cggcctgggc ctgatcctgc
    11701 tgcagaaggc cgtgtccggc ggccgcccga gccgctggat cgcaccggcc catgtcgcgg
    11761 ccgcgctgcg ccacctcgac gtcgtcggca acggcccgca ggtgcgcgcg cagatgcagg
    11821 ggcgcacggc cgagctcgag gcgctgggca tcacgcgcga ggccggccgc ttcgaccgct
    11881 tcctgcagca cgacctgcgc ctgcccgcca gcggcgtgca ggtgcgcgtg gcctggcgcc
    11941 aaccgatcgc cgacgcgccg gtgtgggact tctatcgcac cgccgacatc gacgcgctgc
    12001 tggccgccat cggcgacccg ccgccctatc cggacgccga tgaactggtc gagcacgtcg
    12061 agcgcctgct ggcgcgcgcc gagtccggcc atccggagca cgtcggctcg ctcgtgcagc
    12121 gcctgcaggc cgaggccgcg cggccgcagg ccttcgccga cctgaaggcc tcgctcgtgc
    12181 gcgcccagga tcgctgggag cgccacgtcg ccgagctgaa cgcgctggcg ggcctgaact
    12241 ccgagctcgc gttccagggc tatcccgaca gcttcgagct cgcgcgccga ctgcagcgca
    12301 cggtcacgct gtacgtcggg ccgcccaaca gcggcaagac gcacgccgcg ttcgagcagc
    12361 tggccgccgc gcacgacggc tgctacctcg cgccgctgcg cctgctggcg ctcgagggcc
    12421 gcgaccgcct cgtcgcgcgc ggcgtcccca cgtcgctgct gaccggcgag gagaacgacc
    12481 ccgcggaggg cgcacgcgtc gtctccagca ccatcgagat ggtcaacacg tccaggccga
    12541 tcgacgtggc cgtcatcgac gaggcgcaga tgctgttcga cggctcgcgc ggctgggcct
    12601 ggacgcaggc catcgtgggc gtgccggcca acgaggtgat catcatctgc tcggcctacg
    12661 ccgcgccggc catcgagaac ctgctcaagc tgtgcggcga gaactgcaag gtgcgcagct
    12721 tcgagcgcaa gcagcacgtc gagctgctgc ccggcccggt gccgatctcg gccctgaaga
    12781 agggcgacgc ggtcgtcgcc ttcagccgca aggaggtgct gatgctgcgc gaccaggtcg
    12841 ccgccgacgg ccatccggtc tcggtgatct acggcgccct cccgcccgag gtgcggcgcc
    12901 gcgaggccga gcgcttcgcc cacggccacg cgcacatcct ggtggccacc gacgcgatcg
    12961 gcatgggcct gaacctgccg atccgccgcg tgctgttctc gacgctcaag aagttcgacg
    13021 gcatcggcga ccggctgctg gacgagtccg aggtgcacca gatcgccggc cgcgccggcc
    13081 gctacggcct gcacgaggag ggctttgccg gcgtgctgcg cgaggccgag cccgccgcgc
    13141 tgcgcacgct caaggacctg ctgcccaggc agccgcgtgc gccgcgcgac ttcaaggcct
    13201 tcgtcgcgcc caactggtgg cacgtcgaca ccatcgccac ccggctgggc ctggccaggc
    13261 tgcgcgcggt gctggacgtg ttcgtcgacc agctgcgcct ggacgatgcg cacttcgccg
    13321 tcgccgagct cgagcagatg ctggagctgg ccgagcagct ggaccacaac gcgggcaagc
    13381 tgacgctcaa gcagcgcttc atctacgccc agccggcggt ggacacgcgc accgaaaccc
    13441 aggtgcaggc ctttcccgaa tgggcctgga accacgccaa cgccggccag gccggcacgc
    13501 cgtggttcct gtccgaggtc gacgagcaca gccgcctgga caacatggaa aaggccctgc
    13561 gctcgtgcac cctgtggctc tggctcgacc tgcgtttccc cggcacctat ggccacctcg
    13621 aggaggtgat cgacctgcgc acacggctca acgagggcat cgagcggcat ctgaagggca
    13681 agaagccgtt gtggcagcag aggggccggc ggtagcggtc gagcgctccg aggcgcgtcg
    13741 gtgccagagg ggtctggcct ttcctcgggt cgcgccgact cgctcatcgc agcggcagcc
    13801 tggaccgcga ttcgaggcgg aggcccgacc ccgaacattc tctggggatc ggggtcaggc
    13861 ctttgccttc attcgcgact ggagtaacgc actgcgatcg acgttgcgcc gcgaatgaag
    13921 gagaggccgg acccctccgg cgacatcgga cgtgctcggt tcagagcgcc ggcgcgccga
    13981 gctgcagcgc aggcgcggcc gccgcgaagg ccggcaggcg ttcgcactcg gccaggatgc
    14041 gcgtggacac cgggatgccg gcgacgtcga tcttgagcac gcgcgtgacc atgaacaggc
    14101 tggccaggca gatgtcggcg atggtgggct ggtcgccgtg gcagaagcgg ccggtgccgg
    14161 gctcggtggc caggcgctgt tcgacggcgc gcaggccgct gcccagccag tgcacgagcc
    14221 aggcgcgcga ggcgtcggcg tcgaacggat gggtgtgttc ctcgacgacc tggttgggcg
    14281 gccattgccg ctgcagcacc tcgtcgacgc cgcgcagcag cgggtgggca cggaaggcgt
    14341 cgtaggtggc gccgctgagc gtcgcgttgt ccatggcg
//
LOCUS       DATCRO010000403         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1118088, whole genome shotgun sequence.
ACCESSION   DATCRO010000403 DATCRO010000000
VERSION     DATCRO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118088"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1362
                     /locus_tag="VIN75_10895"
     CDS             91..1362
                     /locus_tag="VIN75_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8974715.1"
                     /translation="MNAVPSPFPDALLPAGAGAAGSAASRATSDYLGFRLASHFQPIF
                     SLTHHRAVGHEALLRPTAIGTGAALEPMDLFASADGDEARVALDRAALRQHLAAYARR
                     DADEWLFLNMHPRSLGSPAGTGIRELAAALAEHGLRPGQVVIEVLESTLPDDPDFDHR
                     IDELRELGCLVSLDDFGAGHSNFDRVFRLRPEIVKLDRSVVARAEVDAHARRIASQMV
                     SLLHECGCLVLMEGIETDEGAYTALRCDVDFVQGYHFGRPAAGLAGITGLHALRTAWD
                     RFDRQSASDDRQWQAQMAPYKQSIELASVLLAGGASLDEACRRFLSLPGAEMCYLLDR
                     QGRQVVGNALRAGLAHQQIERVERFGPLQDTRHARWSRRAYFRNAEAAPNFAQVTRPY
                     MTLQGSRMCVTVSIQFDMGGRSLILCGDASL"
     gene            1405..1968
                     /locus_tag="VIN75_10900"
     CDS             1405..1968
                     /locus_tag="VIN75_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY8974716.1"
                     /translation="MALKATIHKVRLQLADMDRNIYGDHELTIARHPSETDERMLMRV
                     LAYALNVPADDERGKLELAKSLWDVDEPDLWQVDLTGQVVHWIEVGQPDDRRLAKASS
                     RAEIVTIYSYIASTPIWWAALEGKLIRAPKVAVWSVDPEASQALARMCARTMAWQITV
                     QDGTVWVSDTVNTVEITPKLLKARDER"
     gene            complement(1978..2865)
                     /locus_tag="VIN75_10905"
     CDS             complement(1978..2865)
                     /locus_tag="VIN75_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8974717.1"
                     /translation="MTSSTASHPLAARLTAFAPLLFVVLWSTGFIGAKLGLPDCEPLT
                     LLTLRYAAVLVLMGAVALATRAPWPASAAQWLHISVSGLLIHAVYLGGVFTAIRLGLP
                     AGVTALVVGLQPVLTALGAGLLLRERVRAAQWAGLALGLAGVVLVVARKVQGGPAAAE
                     LLLPAVIALAGITMGTLYQKRFCASFDLRTGSVVQFLPSLVVTGLAAWRTETMVVHWT
                     PAFVFALGWLVLVLSLGAVSLLNVLIRSGSAVNVASLFYLTPPTTALIAWAMFGETLT
                     GLALAGMGLAVLGVWLARK"
     gene            complement(3114..>4465)
                     /locus_tag="VIN75_10910"
     CDS             complement(3114..>4465)
                     /locus_tag="VIN75_10910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY8974718.1"
                     /translation="PGVPRIALTATADEHTRADIVHRLQLESARAFVSSFDRPNIRYT
                     IVEKDDARAQLLRFLRDEHEGEAGIVYCQSRKKVEETAAWLESEGIRALPYHAGLDAD
                     VRRRHQDRFLREDGLVMCATIAFGMGIDKPDVRFVAHLDLPKNIEGYYQETGRAGRDG
                     EAADAWMTYGLADVVNQRRMIDESPANDEFKKLQRGKLDALLSLAEAHDCRRVRLLAY
                     FGERGSPCGNCDNCLNPPATWDATEAARMALSTVYRFRQHSGLSFGAGHLIDVLRGKV
                     TDKTTQHGHGELSTFGIGAKWSEQQWRGVIRQLLSLGHLQSEGEYNTLVLADSARAVL
                     KGEVPILLREARELPKGSSRTRRASGGSSGRSTSAPIKLDAAGDQRFAALRAWRSEVA
                     REHNLPAYIVFNDATLAQMAQQAPSSLDELSGISGVGAKKLEAYGREILRVLEAAAS"
BASE COUNT          644 a         1620 c         1600 g          601 t
ORIGIN      
        1 gcccgcaagg cgccggaccg cccgcttgcg cgccgcgccc gccacgaacg gtcgcccgcg
       61 cccgtccgcc gcgccaccgc cttcccgccc atgaacgccg tcccctcgcc ctttcccgac
      121 gcactgctgc ccgccggcgc cggagcggcg ggcagcgcgg cgtcgcgcgc cacgtccgac
      181 tacctgggtt ttcgcctcgc ctcgcacttc cagccgatct tcagcctgac gcaccatcgc
      241 gccgtcggcc acgaggcgct gctgcggccc acggcgatcg gcacgggcgc cgcgctggag
      301 ccgatggacc tgttcgcgag cgccgacggc gacgaggcgc gcgtggcgct cgatcgcgcc
      361 gcgctgcgcc agcacctggc cgcgtacgcg cgccgcgacg ccgacgagtg gctgttcctg
      421 aacatgcatc cgcgctcgct cggttcgccg gccggcacgg gcatccgcga gctcgccgcc
      481 gcgctcgccg agcacggcct gcgcccgggc caggtggtga tcgaggtgct ggaatcgacg
      541 ctgcccgacg accccgactt cgaccaccgc atcgacgaac tgcgcgaact gggctgcctc
      601 gtctcgctgg acgacttcgg cgccggccac tccaacttcg accgcgtgtt ccggctgcgt
      661 cccgagatcg tcaagctcga tcgcagcgtg gtggcgcggg cggaggtcga tgcgcacgcg
      721 cgccgcatcg cctcgcagat ggtgtcgctg ctgcacgagt gcggctgcct ggtgctgatg
      781 gaaggcatcg agaccgacga aggcgcgtac accgcgctgc gctgcgacgt cgacttcgtg
      841 cagggctacc acttcggccg gccggccgcg ggcctggccg gcatcacggg cctgcacgcg
      901 ctgcgcaccg cctgggaccg cttcgaccgc cagtcggcca gcgacgaccg gcagtggcag
      961 gcgcagatgg cgccctacaa gcagtcgatc gaactggcca gcgtgctgct ggccggcggg
     1021 gcgtcgctgg acgaggcctg ccggcgcttc ctgtcgctgc cgggcgccga gatgtgctac
     1081 ctgctggacc gccagggccg ccaggtggtg ggcaacgcgc tgcgcgcggg gctggcgcac
     1141 cagcagatcg agcgcgtcga gcgcttcggc ccgctgcagg acacccgcca cgcccgctgg
     1201 tcgcgccgcg cctacttccg caacgccgag gccgcgccca acttcgcgca ggtcacgcgg
     1261 ccgtacatga cgctgcaggg ctcgcgcatg tgcgtcacgg tgtcgatcca gttcgacatg
     1321 ggcgggcgct cgctgatcct gtgcggggac gcgtcgctgt agtccggcgg gagaggcgtg
     1381 gcgattgcag ggacaatcgg ccccatggcc ctcaaagcaa cgatccacaa ggtccgtctg
     1441 cagctcgcgg acatggaccg caacatctac ggcgaccacg aactgacgat cgcccgccac
     1501 ccgtcggaaa ccgacgagcg catgctcatg cgcgtcctgg cctacgcgct caacgtgccg
     1561 gccgacgacg agcgcggcaa gctcgagctc gccaagagcc tgtgggacgt cgacgagccc
     1621 gatctctggc aggtcgacct caccggccag gtcgtgcact ggatcgaggt gggccagccc
     1681 gacgaccgcc gcctggccaa ggcgagcagc cgcgccgaga tcgtcaccat ctacagctac
     1741 atcgccagca cgccgatctg gtgggccgcg ctggaaggca agctgatccg cgcgcccaag
     1801 gtggcggtgt ggtcggtcga ccccgaggcc tcgcaggcgc tcgcccggat gtgcgctcgc
     1861 acgatggcct ggcagatcac ggtgcaggac ggcacggtgt gggtgtcgga caccgtcaac
     1921 acggtcgaga tcacgccgaa gctgctcaag gcgcgggacg agcgataagg ccggtgctca
     1981 cttgcgcgcc agccacaccc ccagcaccgc cagccccatg ccggccagcg ccagtcccgt
     2041 cagcgtctcg ccgaacatcg cccacgcgat cagcgcggtg gtgggcggcg tcaggtagaa
     2101 caggctggcc acgttgacgg cgctgccgct gcgaatgagc acgttgagca ggctcacggc
     2161 gcccagcgac agcacgagca ccagccagcc cagcgcgaac acgaacgcgg gcgtccagtg
     2221 aaccaccatc gtctcggtgc gccacgcggc caggccggtg acgaccaggg atggcaggaa
     2281 ctgcacgacc gagccggtgc gcaggtcgaa gctggcgcag aagcgcttct ggtacagcgt
     2341 gcccatcgtg atgcccgcga gcgcgatgac cgccggcagc agcagctccg ccgcggccgg
     2401 gccgccctgg accttgcgcg ccacgacgag caccacgccc gccaggccca gcgcgagccc
     2461 ggcccattgc gcggcgcgca cgcgttcgcg caggagcagc ccggcgccga gcgccgtcag
     2521 caccggctgc aggccgacga ccagcgccgt cacgcccgcc ggcagcccca gccggatcgc
     2581 ggtgaacacg ccgccgaggt acacggcatg gatcaacagc cccgacacgc tgatgtgcag
     2641 ccactgcgcc gccgacgcgg gccacggcgc gcgcgtggcg agcgcgaccg cgcccatcag
     2701 caccagcacc gcggcatagc gcagcgtgag cagcgtgagc ggctcgcaat cgggcaggcc
     2761 cagcttggcg ccgatgaagc cggtgctcca gagcacgacg aacagcagcg gcgcgaacgc
     2821 ggtgaggcgg gcggcgaggg gatgcgaggc ggtggaggac gtcatgcgtg gcgcgcatca
     2881 tcgcgcggat gggacggcgc tgccggagcg agggcaatcg gccttggcga ggcgtcgtgg
     2941 caggcggagg tggatcctgc gactgcgcgc aggatgacag gcttccggcg cagcctgcgg
     3001 ggctgcctcc cggtcggcgc cgatgtggga gacgacgttg ccgcagggcg ccttccaccc
     3061 tcgcatgtca tcctgcgcgc agtcgcagga tcgccgcccg gcacgacgcc gcgtcagctc
     3121 gcagccgcct ccagcacccg caggatctcg cgcccgtacg cctcgagctt cttggcgccc
     3181 acgccgctga tgcccgacag ctcgtccagc gacgacggcg cctgctgggc catctgcgcc
     3241 agcgtggcgt cgttgaagac gatgtaggcc ggcaggttgt gttcgcgcgc cacctcggag
     3301 cgccaggcgc gcagcgcggc gaagcgctgg tcgccggcgg cgtcgagctt gatcggcgcg
     3361 gacgtgctgc ggccgctcga ccccccgctt gcgcggcgcg tgcggctcga gcccttgggc
     3421 agttcgcgcg cctcgcgcag caggatcggc acctcgccct tgagcacggc gcgcgcgctg
     3481 tcggccagca ccagcgtgtt gtattcgcct tccgactgca ggtggcccag cgacagcagc
     3541 tggcggatca cgccgcgcca ttgctgctcc gaccacttcg cgccgatgcc gaaggtcgac
     3601 agctcgccgt ggccgtgctg cgtggtcttg tcggtgacct tgccccgcag cacgtcgatc
     3661 agatggccgg cgccgaagct caggcccgag tgctggcgga agcggtagac ggtggacagg
     3721 gccatgcgcg cggcctcggt ggcgtcccac gtggccggcg ggttgaggca gttgtcgcaa
     3781 ttgccgcagg gcgaaccgcg ttcgccgaag taggccagca gccgcacgcg ccggcagtcg
     3841 tgcgcctcgg ccaacgacag cagcgcgtcg agcttgccgc gctgcagctt cttgaactcg
     3901 tcgttggcgg ggctctcgtc gatcatgcgg cgctggttga cgacgtcggc caggccgtac
     3961 gtcatccagg cgtcggccgc ctcgccgtcg cggcctgcgc ggcccgtctc ctggtagtag
     4021 ccctcgatgt tcttgggcag atcgaggtgg gcgacgaagc gcacgtcggg cttgtcgatg
     4081 cccatgccga acgcgatggt ggcgcacatg accaggccgt cctcgcgcag gaagcggtcc
     4141 tggtggcgcc ggcgcacgtc ggcgtcgagg ccggcgtggt agggcagcgc gcggatgccc
     4201 tcggactcga gccaggccgc ggtctcctcg accttcttgc gcgactggca gtagacgatg
     4261 ccggcctcgc cctcgtgctc gtcgcgaagg aagcgcagca gctgcgcgcg cgcgtcgtcc
     4321 ttctcgacga tggtgtagcg gatgttgggt cggtcgaagc tggagacgaa ggcgcgcgcc
     4381 gactccagct gcaggcggtg gacgatgtcc gcgcgggtgt gctcgtcggc ggtcgcggtc
     4441 agtgcgatgc gaggcacgcc ggggt
//
LOCUS       DATCRO010000404         7079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_116752, whole genome shotgun sequence.
ACCESSION   DATCRO010000404 DATCRO010000000
VERSION     DATCRO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116752"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..880)
                     /locus_tag="VIN75_10915"
     CDS             complement(215..880)
                     /locus_tag="VIN75_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY8974719.1"
                     /translation="MHRISTTRPLRRAAAAAALCALAAAAQAQSVAFSGMLGDKALLI
                     IDGQAKGVAVGSTVSGVKLVRIDGTQAQIEANGKTQTLRLGGTAVVAGDPGNGGGGGR
                     IVLPVGSGGHYTGLASINGHPVQFVVDTGATSVSMGADVASQLGLDYRDSTGGAAMTA
                     NGAIAVRKITLHKVTVGDVTVYNVEAMVVPQSMPLVLLGNSFLSHFQMHSDSSTLVLD
                     KKN"
     gene            complement(1005..1907)
                     /locus_tag="VIN75_10920"
     CDS             complement(1005..1907)
                     /locus_tag="VIN75_10920"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY8974720.1"
                     /translation="MAATPPRILSEPRVVGDVRATLGEGLCWSPSTKSLWWVDILEHR
                     LYRDAIVGAHEAWSFDETISAVAERANGGGLAVTLRRGLALFDPASRALVRLDEPERE
                     REGNRFNDGKCDARGRFWGGTMDFAVRAPSGALYRFDAEGRAQRAIDLGWIVTNGPTW
                     TQDGRTMFVNDTVQRRVVAYAFDPDTGAVGASREWLALPAADGHPDGMTTDAAGRIWI
                     AHWGGACVTCHDPVTAQELARIALPTDHITNVAFGGPDMTTLFVSSAKFELSDAQLAA
                     QPLAGALFAVETDAPGLAAHLYAG"
     gene            complement(1918..3639)
                     /gene="araD"
                     /locus_tag="VIN75_10925"
     CDS             complement(1918..3639)
                     /gene="araD"
                     /locus_tag="VIN75_10925"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEY8974721.1"
                     /translation="METKKPVRRSQAWFGKNDRDGFVHRSWIKNQGYPHDELDGRPVI
                     GICNTWSELTPCNGHFRELAEFVKRGVYEAGGFPLEFPVMSLGETQLRPTAMLFRNLA
                     SMDVEESIRGNPIDGVVLLMGCDKTTPALMMGAASCNLPTIGLSGGPMLNGKFRGQDI
                     GSGTGVWKMSEMVRAGEMTQEDFTAAESCMHRSKGNCMTMGTASTMASMVEALGMSLP
                     ENAAIPAADTRRNRLAQLTGRRIVDMVKEDLTMSRILTREAFENAIRTNAAIGGSTNA
                     VIHLLAIAGRIGIDLKLADWDKLGAQVPCLVDLQPSGKFLMEDFFYAGGLPAVLREIQ
                     HLLHLDAPTVNGKTIGENIAAAPCWNREVIRPLDKPFMPDAGIAVLRGNLAPDGAVIK
                     PSAASAHLLKHRGRAVVFETIEEFHAKIDDESLDIDENCIMVLKNCGPKGYPGMAEVG
                     NMPLPPKVLRKGITDMVRISDARMSGTAYGTVVLHTSPEAAAGGPLALVQNGDMIELD
                     VPARRLHVEVSDEELARRRANWKPLDAPKRGWQKLYVDHVQQAHLGADLDFLVGASGA
                     GIPRDSH"
     gene            3799..4563
                     /locus_tag="VIN75_10930"
     CDS             3799..4563
                     /locus_tag="VIN75_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8974722.1"
                     /translation="MNPARFPSLQGRAVFVTGGGSGIGAAMVEAFAAEGARVAFVDIA
                     AEASAELARRIGSRGHPAPWWRACDVRDVPALQKAIADAAAVLGDFHALVNNVASDDR
                     HTLESVTTEYWDNRMAINERPAFFAIQAVVPGMQRLGQGAIVNLGSTGWQSKASEYPC
                     YAIAKSSVNGLTRGLAKSLGAHRIRINTVSPGWVMTERQVSKWLTPAGEDDIARNQCL
                     PDKLLPEDIARMVLFLAADDGRMCTAQEFTVDAGWV"
     gene            4641..5363
                     /locus_tag="VIN75_10935"
     CDS             4641..5363
                     /locus_tag="VIN75_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974723.1"
                     /translation="MFFGRKRPSVRLVRAWCSPFGEDGPAGELLALVDEVLASTALQP
                     LEGQINFGYTQPMSSLRRALAKMKPVALDALTIQDEAGDVICSFGAESASVGDRVRAF
                     EIFALLPPASEPAQDKLLRLLMRHGMHYGYARALGAALDPVTETPRKRGFFDQTIEVA
                     GGRADWLVPEMDVRAGAIRGLYPLNVFSAVGLARLAGSGLRLPPSAPTLGDQLWRPTV
                     IEQSELQRSNPSYRGFLHFGDD"
     gene            complement(5471..6442)
                     /locus_tag="VIN75_10940"
     CDS             complement(5471..6442)
                     /locus_tag="VIN75_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8974724.1"
                     /translation="MSTADTHWFIRARLKTRQLLLLVAMEEEGNIHGAAQVLNMTQPA
                     ASKLLKDLEDMLEVRLFDRLPRGVRPTWYGETMIRHARMALASLSQAHEEIDALKAGR
                     FGSVAIGTITAPAITLLPDAVAQVKAAHPSLRVGLQVETSDVLIDRLTQGKLDMVVGR
                     LFARHDKSDLRYEALVEEPIAAVVRPGHPLMEHASLKLPDLSQYGWIVPPTGSVLRHR
                     FELMFHEDGLASPSDLIETTALLFMTKMLGQSDLIGVVASDVALYYQEHGLVAILPVE
                     LPCKMDAFGLITRTDRLLAPAAQVMLKSIKAVAHDVYGTVLAVDETE"
     gene            6675..>7079
                     /locus_tag="VIN75_10945"
     CDS             6675..>7079
                     /locus_tag="VIN75_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8974725.1"
                     /translation="MLAVVAFGVVTLGAAQAQDKGTVGIAMPTKSSARWIADGDNMVK
                     VLQARGYKTDLQYAEDDIPNQLAQIENMVTKGDKVLVIASIDGTTLSNVLQKAADKGI
                     KVIAYDRLIKGSKNVDYYATFDNFQVGVLQADS"
BASE COUNT         1032 a         2420 c         2528 g         1099 t
ORIGIN      
        1 gccgccgaac acgatcggcg cgatttgcag gccggtgcgg ccgaggggac gaaattgcat
       61 gggagagctc ctgcgcgcac ggggggcgcg ggtcgaacgg gccggccggg gccggcgtca
      121 ccggcgctcg cggcaaggca cgcgcccgcc ggcgcggcgg catgcgcctg ctgtcagctc
      181 tttccgtttg acacaggcgg ccgcggcgcg ttgctcagtt cttcttgtcc aacaccagcg
      241 tgctcgagtc gctgtgcatc tggaagtgcg acaggaagct gttgccaagc agcaccagag
      301 gcatggactg cggcacgacc atcgcctcga cgttgtagac cgtgacatcg ccgaccgtca
      361 ccttgtgcag cgtgatcttg cgcacggcga tcgcgccgtt ggccgtcatc gccgcgccgc
      421 cggtgctgtc gcggtagtcg agcccgagct gcgacgcgac gtccgcgccc atcgacaccg
      481 acgtggcgcc ggtgtccacg acgaactgca ccgggtggcc gttgatggac gcgagcccgg
      541 tgtagtgccc gcccgagccg accggcagca cgatgcgccc accgccgccg ccgttgcccg
      601 gatcgcccgc gaccacggcg gtgccgccca ggcgcagcgt ctgcgtcttg ccgttggcct
      661 cgatctgcgc ctgcgtgccg tcgatgcgca ccagcttgac gcccgagacg gtgctgccca
      721 cggccacgcc cttggcctgc ccgtcgatga tcagcagggc cttgtcgccc agcatgccgc
      781 tgaaggcgac ggactgggcc tgcgccgcgg cggcgagcgc gcacagtgcg gcggcggccg
      841 cggcacggcg cagcgggcgc gtggtcgaaa ttcgatgcat gggaactccc tcggtcggcg
      901 atggcgctcc gcgcgcagcg ggacggccgt tcatcgatgg aagtctagtc gctctggcga
      961 ccaccgggcg cccgggggcg cggcgatcgt ggcgcagttc gcgttcagcc cgcgtagaga
     1021 tgggccgcca ggcccggcgc atccgtctcc accgcgaaca gcgcgccggc cagcggctgc
     1081 gccgcgagct gggcgtcgct cagctcgaac ttcgcgctgc tgacgaacag cgtcgtcatg
     1141 tccggtccgc cgaaggcgac gttggtgatg tggtcggtgg gcagcgcgat gcgcgccagc
     1201 tcctgcgcgg tgacggggtc gtggcaggtc acgcacgcgc cgccccagtg cgcgatccag
     1261 atccggccgg cggcatcggt ggtcatgccg tcggggtggc cgtcggccgc gggcagcgcc
     1321 agccactcgc gcgacgcgcc cacggcgccg gtgtcgggat cgaacgcgta ggccacgacg
     1381 cggcgttgca ccgtgtcgtt gacgaacatc gtgcggccgt cctgcgtcca ggtggggccg
     1441 ttggtgacga tccaacccag gtcgatcgcg cgctgcgcgc ggccctcggc gtcgaagcgg
     1501 tacagcgcgc cgctgggcgc gcgcaccgcg aagtccatcg tgccgcccca gaagcgcccg
     1561 cgcgcgtcgc acttgccgtc gttgaagcgg ttgccctcgc gctcgcgctc gggctcgtcc
     1621 aggcgcacga gcgcgcgcga ggccggatcg aacagcgcca ggccgcggcg cagcgtgacc
     1681 gccagcccgc cgccgttggc gcgttcggcg acggccgaga tcgtctcgtc gaaggaccag
     1741 gcctcgtgcg cgccgacgat cgcgtcgcgg tagaggcgat gctcgaggat gtcgacccac
     1801 cacagcgact tcgtggacgg cgaccagcac aggccttcgc cgagcgtcgc gcgcacgtcg
     1861 ccgacgacgc gcggttccga gaggatacgg ggtggggtag ctgccatcga cgggtgatca
     1921 gtgcgagtcg cgcgggatgc cggcgccgct ggcgccgacc aggaagtcca gatcggcccc
     1981 caggtgcgcc tgctgcacgt ggtcgacgta gagcttctgc cagccgcgct tgggcgcgtc
     2041 cagcggcttc cagttcgcgc ggcggcgggc cagttcctcg tcgctgacct cgacgtgcag
     2101 ccggcgggcg gggacgtcca gctcgatcat gtcgccattc tgtaccagtg cgagcgggcc
     2161 gccggccgcc gcctcgggcg aggtgtgcag cacgacggtg ccgtaggccg tgccgctcat
     2221 gcgggcgtcg gagatgcgca ccatgtccgt gatgcccttg cgcagcacct tgggcggcag
     2281 cggcatgttg ccgacctcgg ccatgcccgg gtagcccttg gggccgcagt tcttgagcac
     2341 catgatgcag ttctcgtcga tgtccagcga ctcgtcgtcg atcttcgcgt ggaactcctc
     2401 gatcgtctcg aagacgacgg cacggccgcg gtgcttgagc aggtgtgcgg aggccgccga
     2461 cggcttgatg acggcgccgt ccggcgcgag attgccgcgc agcacggcga tgccggcgtc
     2521 gggcatgaag ggcttgtcca gcgggcggat cacctcgcgg ttccagcagg gcgcggcggc
     2581 gatgttctcg ccgatcgtct tgccgttgac ggtgggcgcg tccaggtgca gcaggtgctg
     2641 gatctcgcgc agcaccgcgg gcaggccgcc ggcgtagaag aagtcctcca tcaggaactt
     2701 gcccgagggc tgcaggtcga cgaggcaggg cacctgcgcg ccgagcttgt cccagtcggc
     2761 cagcttcagg tcgatgccga tgcgcccggc gatcgccagc aggtggatca ccgcgttggt
     2821 cgagccgccg atcgccgcgt tggtgcggat cgcgttctcg aaggcctcgc gggtgaggat
     2881 cctggacatc gtcaggtctt ccttgaccat gtcgacgatg cggcggcccg tgagctgcgc
     2941 aaggcggttg cggcgcgtgt cggccgcggg gatcgccgcg ttctcgggca gcgacatgcc
     3001 cagcgcctcg accatcgacg ccatcgtcga ggccgtgccc atggtcatgc agttgccctt
     3061 ggagcggtgc atgcagctct cggcagcggt gaaatcctcc tgcgtcatct cgccggcccg
     3121 gaccatctcg ctcatcttcc agacgccggt gcccgagccg atgtcctggc cgcggaactt
     3181 gccgttgagc atcgggccgc cggacagccc gatggtgggc aggttgcagc tggccgcgcc
     3241 catcatcagc gcgggagtcg tcttgtcgca tcccatgagc agcacgacgc cgtcgatggg
     3301 gttgccgcgg atcgattcct cgacgtccat gctggccagg ttgcggaaca gcatcgcggt
     3361 gggccgcagc tgcgtctcgc cgagcgacat caccgggaac tcgagcggga agccgccggc
     3421 ctcgtagacg ccgcgcttga cgaattcggc gagctcgcgg aagtgcccgt tgcagggcgt
     3481 gagctcgctc caggtgttgc agatgccgat gaccggacgg ccatccagct cgtcgtgcgg
     3541 gtaaccctgg ttcttgatcc agcttcgatg cacgaacccg tcgcgatcgt tctttccgaa
     3601 ccacgcctgg ctgcggcgga cgggcttctt tgtctccatt gcgcttctct tctcgacttg
     3661 acggggcgca tcgtatcgcc gcacactgat attggaaaaa taaagatcag cgcaattgac
     3721 attgcaaaaa aggcatagat gacccacgca tcgaactctt ccgccgcgac gccggcgttc
     3781 acgcacgccg cggggcccat gaaccccgcg cgattcccca gcctgcaggg ccgcgccgtg
     3841 ttcgtcacgg gcggcggctc gggcatcggc gccgcgatgg tcgaggcgtt cgcggccgag
     3901 ggcgcacgcg tcgcgttcgt cgacatcgcg gccgaggcca gcgccgagct cgcgcgccgc
     3961 atcgggagcc gcggccatcc cgcgccgtgg tggcgcgcct gcgacgtgcg cgacgtgccc
     4021 gcgctgcaga aggcgatcgc cgacgcggcc gccgtgctcg gcgacttcca cgcgctcgtg
     4081 aacaacgtgg ccagcgacga tcgccacacg ctcgaatcgg tgacgaccga gtactgggac
     4141 aaccgcatgg cgatcaacga gcgcccggcc ttcttcgcga tccaggccgt cgtgccgggc
     4201 atgcagcggc tggggcaggg cgccatcgtc aacctgggct cgacgggctg gcagagcaag
     4261 gccagcgagt acccgtgcta tgcgatcgcc aagtcctcgg tcaacggcct gacgcggggc
     4321 ctggccaagt cgctgggcgc gcaccgcatc cgcatcaaca ccgtctcgcc cggctgggtg
     4381 atgaccgagc gccaggtctc gaagtggctg acgcccgcgg gcgaggacga catcgcgcgc
     4441 aaccagtgcc tgcccgacaa gctgctgccc gaggacatcg cccgcatggt gctgttcctc
     4501 gcggcggacg acgggcgcat gtgcactgcc caggagttca cggtcgacgc cggctgggtg
     4561 tgacgtctgg acactgagcg cccctgcggc tcggggacaa tggttgcgga gccacaacgg
     4621 gccgcgaggc cgcccagatg atgttcttcg gcaggaagcg gcccagcgtc aggctggtcc
     4681 gggcctggtg ttcccccttc ggcgaggacg gccctgcggg tgagctgctc gcgctcgtcg
     4741 acgaggtgct ggcgtcgacc gcgctgcagc cgctcgaagg ccagatcaat ttcggctaca
     4801 cgcagccgat gtcgtcgctg cgccgcgcgc tggccaagat gaagccggtg gcgctggacg
     4861 cgctgacgat ccaggacgag gccggggacg tcatctgcag cttcggcgcg gagagcgcgt
     4921 cggtgggcga ccgcgtccgc gcgttcgaga tcttcgcgct gctgccgccc gcctcggagc
     4981 cggcgcagga caagctgctg cgcctgctga tgcgccacgg gatgcactac ggctacgccc
     5041 gcgcgctcgg cgcggcgctg gacccggtca ccgagacgcc ccgcaagcgc ggtttcttcg
     5101 accagacgat cgaggtcgcc ggcggccgcg ccgactggct ggtgcccgag atggacgtgc
     5161 gcgccggcgc catccgcggg ctgtacccgc tcaacgtgtt ctcggccgtc ggcctcgcgc
     5221 gcctggccgg ctcgggcctg cgcctgccgc cctcggcgcc gacgctgggc gaccagctgt
     5281 ggcgcccgac ggtcatcgag cagtcggagc tgcagcggtc caatccgtcg taccgcggct
     5341 tcctgcattt cggggacgat tgaccgggga gcgaaacgac tggcccgcgc tccgggtggc
     5401 gcgcgcgagg gtgagggata cgcgctttca gggcgccttg tggtccgacg aacggcgtgg
     5461 cggccgaccc tcattcggtc tcgtcgaccg ccagcacggt cccgtagacg tcatgggcga
     5521 ccgccttgat cgacttgagc atcacctgcg cggcgggcgc cagcaggcgg tcggtgcgcg
     5581 tgatcaggcc gaacgcgtcc atcttgcagg gcagctccac cggcaggatc gcgaccaggc
     5641 cgtgctcctg gtagtacagc gcgacgtccg aggcgacgac gccgatcagg tcgctctggc
     5701 ccagcatctt ggtcatgaac agcagggcgg tcgtctcgat gaggtcgctc ggggacgcga
     5761 ggccgtcctc gtggaacatc agctcgaagc ggtgccgcag cacgctgccg gtcggcggca
     5821 cgatccagcc gtactgcgag aggtcgggca gcttcagcga ggcgtgctcc atcagcgggt
     5881 ggccagggcg cacgacggcg gcgatcggct cctcgacgag cgcctcgtag cgcaggtcgc
     5941 tcttgtcgtg gcgcgcgaag aggcggccga cgaccatgtc gagcttgccc tgcgtgaggc
     6001 ggtcgatcag cacgtcgctg gtctcgacct gcaggcccac gcgcagcgac gggtgggcgg
     6061 ccttgacctg cgccaccgcg tcgggcagca gcgtgatggc gggcgcggtg atggtgccga
     6121 tcgccaccga gccgaagcgg ccggccttga gcgcgtcgat ctcctcgtgc gcctggctca
     6181 ggctggccag cgccatgcgc gcgtggcgga tcatggtttc cccgtaccac gttgggcgca
     6241 cgccacgcgg caggcgatcg aacaggcgca cttccagcat gtcctccagg tctttcagca
     6301 gcttggaggc ggccggctgg gtcatgttca ggacctgcgc ggcgccgtgg atgttgcctt
     6361 cctcctccat ggccacgagg aggagcagct ggcgcgtctt gagccgcgcg cggatgaacc
     6421 agtgggtgtc ggcggtggac atgtttccgg ggacggggcg gccggcggtg atgcgatgcc
     6481 cgaatgtata tggcaagacg ccaaaaatct attaggcagt tatctcagcc gttcgtacca
     6541 tcgcggccat cgagggcttt ccggtgcttc gtcgcggccg cgtgccgccg ccgccggagg
     6601 cgcccgacga tgatcgaaaa cccgagccga tgacggccga tctttggaga caagacgtga
     6661 aattcctcaa gacagtgctg gccgtcgtgg ccttcggcgt ggtcaccctc ggcgccgccc
     6721 aggcgcagga caagggcacc gtgggcatcg cgatgccgac gaagtcgtcc gcgcgctgga
     6781 tcgccgacgg cgacaacatg gtcaaggtgc tgcaggcccg cggctacaag accgacctgc
     6841 aatacgccga ggacgacatc ccgaaccagc tcgcgcagat cgagaacatg gtcaccaagg
     6901 gcgacaaggt cctcgtgatc gcctcgatcg acggcacgac gctgtccaac gtgctgcaga
     6961 aggccgccga caagggcatc aaggtcatcg cctacgaccg cctgatcaag ggctccaaga
     7021 acgtcgacta ctacgccacg ttcgacaact tccaggtcgg cgtgctgcag gccgactcc
//
LOCUS       DATCRO010000405         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_477048, whole genome shotgun sequence.
ACCESSION   DATCRO010000405 DATCRO010000000
VERSION     DATCRO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477048"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VIN75_10950"
     CDS             <1..372
                     /locus_tag="VIN75_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY8974726.1"
                     /translation="APADEAPARVAQPFESRAFAAPAPAPAPAPIVAAPVAPVAPVVP
                     KFDLPIGDLNSLAGSAGLEWVHSNADAVRAAQEAIANTPKAVHVPRERPPVVVVDEGP
                     LVLVETRKDLSQIKLPFDTQA"
     gene            392..928
                     /locus_tag="VIN75_10955"
     CDS             392..928
                     /locus_tag="VIN75_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974727.1"
                     /translation="MTRLTALLRSPRPLAAAMAAVAVLSLAACKGDDAPVTPAGAALV
                     ASAPAVAATNVPPPPVIQSVAATPAAPASMAAGGDLAGVEAQLEGTLTKASACTADAE
                     CRSVAVGAKACGGPTGYRAYSARTASPDSVEALAQHERELAAAQARASHQVSPCFMLA
                     DPGARCEQNKCVTGRAGP"
     gene            1132..1791
                     /locus_tag="VIN75_10960"
     CDS             1132..1791
                     /locus_tag="VIN75_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974728.1"
                     /translation="MNLASLPRTRWLPFALAWLLAFVALEAGAQLPTKPGKYGKTLVA
                     DVVLPPSQLDKMAAFLDTLHASNHGAQNSVAFGETVDWTGDAMTGTPSASPYTIVVQA
                     EVTANQAGNVTTTWKTGWHMESGENRQTLLTGLVETDAKPGQRLTLTGVSTASRFDRD
                     KNVTPVVSFVDAHNATLQRVQVQVWSGMPRASFLDYFGAFRFLLLGVVMLVVVLVFRR
                     I"
     gene            complement(1793..2965)
                     /gene="nagZ"
                     /locus_tag="VIN75_10965"
     CDS             complement(1793..2965)
                     /gene="nagZ"
                     /locus_tag="VIN75_10965"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY8974729.1"
                     /translation="MARKTKKNANKSLPSGEARVVEQLQKADAAPFEAHAPVILDVAG
                     TQLTADDRRRLAHPLTGGAILFGRNWVNRQQVTELVAEMKAIRPDLLVCVDHEGGRVQ
                     RFRTDGFTHLPAMDAYGKRWMDDGRGPAGDGAMAATDSATAAGFVMAAELRSCGVDLS
                     FAPVLDLHFGRSVVIGDRSFHRDARVVTLLAKSLMLGMRQAGMANCGKHFPGHGYVAA
                     DSHTDVPVDARKLAEILRDDARPYEWLSTSLASVMPAHVVYPDVDDKPAGFSAKWLQD
                     ILRGRLGFTGAIFSDDLSMAGARTLAGRELGYIEAGVLAMNAGCDLVLLCNQSLGKGE
                     PLDALLDGLIKARDAGAWLPDSQSERRRLDLLPQTEPLAWDDLMRDPAYLRALDRL"
     gene            complement(2970..3395)
                     /gene="acpS"
                     /locus_tag="VIN75_10970"
     CDS             complement(2970..3395)
                     /gene="acpS"
                     /locus_tag="VIN75_10970"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY8974730.1"
                     /translation="MIFGIGTDLCDVRRITATLQRKGDRFAEKVLGPDEIKVFHARRA
                     KVEGRGIRFLATRFSAKEAFSKAVGMGLHMPMTWRSCEILNERSGKPFIRLHGELAAW
                     FEARNLVAHVTVTDESDYVASFVVVETRPADDAGVQTQS"
     gene            complement(3392..>4032)
                     /locus_tag="VIN75_10975"
     CDS             complement(3392..>4032)
                     /locus_tag="VIN75_10975"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY8974731.1"
                     /translation="ITVHPRPDERHIRAADVFELKKLLQQWPEAEYNIEGNPTQNLMR
                     FIREARPHQATFVPDSEDQFTSDHGWDVARDAARLRPLIEECHALGVRVSLFMDPLPE
                     AMDAVRGLGADRIELYTETYASAHGTPRQAEVLGRFTATAQAALALGLGINAGHDLSL
                     VNLTDFIRAVPGVAEVSIGHALVADALEFGMADTVHRYLRAIVDAESAETLA"
BASE COUNT          546 a         1442 c         1461 g          583 t
ORIGIN      
        1 gccccggccg acgaggcgcc ggcccgcgtc gcccaaccct tcgagtcgcg cgcgttcgcc
       61 gcgccggcgc cggcccccgc gcccgcgccg atcgtcgctg cgccggtcgc accggtcgcc
      121 ccggtcgtgc cgaagttcga cctgcccatc ggcgacctga actcgctcgc gggttcggcc
      181 ggcctggagt gggtgcactc gaacgccgac gccgtgcgcg ccgcgcagga agcgattgcc
      241 aacacgccca aggccgtgca cgtgccgcgc gagcggccgc cggtggtcgt ggtcgacgaa
      301 ggcccgctgg tgctggtcga gacccgcaag gatctcagcc agatcaagct gccgttcgac
      361 acccaggcct gaccattgcc ggagtgatcg gatgacccgc ctgaccgccc tgctccgctc
      421 tccgcgtccg ctggccgcgg ccatggccgc cgtcgcggtc ctgtcgctgg ccgcctgcaa
      481 gggcgacgac gccccggtga ccccggccgg cgccgcgctg gttgcctcgg ctccggccgt
      541 ggccgccacg aacgtgccgc cgccgccggt catccagtcg gtcgccgcga cccctgcggc
      601 gccggcgagc atggcggccg gtggcgacct ggcgggcgtc gaggcgcagc tcgagggcac
      661 gctgacgaag gcctccgcct gcacggccga cgcggaatgc cgctcggtcg cggtcggcgc
      721 caaggcctgc ggcggcccga ccggctaccg cgcgtactcg gccaggaccg cgtcgccgga
      781 cagcgtggaa gcgctggcgc agcacgagcg cgagctcgcg gcggcgcagg ccagggcctc
      841 gcaccaggtc tcgccctgct tcatgctcgc cgacccgggc gcgcgctgcg agcagaacaa
      901 atgcgtgacg ggccgcgccg gcccctgagc tgcgcatgat ccgcgaacgc cgcgaccccg
      961 cccgggctcg cggctttttt tcgtcttgcg ggcagggtcg cctcgacccg tgtcctgccg
     1021 cggtccgcaa aagtgcgacc cactgcacgg gaaacggcgc ttcatccagg tgaccgaagg
     1081 atccacgccg cgcgcgcgga tagagttccg caaactcttc gcaccgcccc gatgaacctc
     1141 gcctccctgc cgcgcacccg ctggctgcct ttcgccctcg cctggctgct ggccttcgtc
     1201 gccctcgagg ccggcgccca gttgcccacc aagccgggca agtacggcaa gacgctggtc
     1261 gccgacgtcg tgctgccgcc gtcgcagctg gacaagatgg ccgccttcct ggacacgctg
     1321 cacgccagca accacggcgc gcagaacagc gtcgcgttcg gcgagacggt ggactggacc
     1381 ggcgacgcga tgaccggcac gccgtccgcc agcccctaca cgatcgtcgt gcaggccgag
     1441 gtcaccgcca accaggcggg caacgtcacc acgacgtgga agacgggctg gcacatggaa
     1501 agcggcgaga accggcagac cctgctgacg ggcttggtgg agacggacgc caagcctggc
     1561 cagcgcctga cgctgacggg cgtctcgacc gcgtcgcgct tcgaccgcga caagaacgtg
     1621 acgccggtgg tcagcttcgt cgacgcgcac aacgccacgc tccagcgcgt gcaggtgcag
     1681 gtctggtcgg gcatgcccag agccagcttc ctggactact tcggcgcctt ccgcttcctg
     1741 ctgctgggcg tggtgatgct ggtggtggtg ctggtcttcc gccggatctg agtcagagcc
     1801 ggtcgagcgc ccgcaggtac gccgggtcgc gcatcaggtc gtcccaggcc agcggctcgg
     1861 tctgcggcag caggtcgagg cggcggcgct cggactggct gtccggcagc cacgcgccgg
     1921 cgtcgcgcgc cttgatcagg ccgtccagca gcgcatcgag cggctcgccc ttgcccaggc
     1981 tctggttgca cagcaggacg agatcgcagc ccgcgttcat cgccagcacg ccggcctcga
     2041 tgtagcccag ctcgcggccg gcgagcgtgc gcgcgccggc catgctcagg tcgtcgctga
     2101 atatcgcgcc ggtgaagccc aggcggccgc gcaggatgtc ctgcagccac ttggccgaga
     2161 agccggccgg cttgtcgtcg acgtcgggat agacgacgtg cgccggcatc acgctggcca
     2221 gcgacgtgga cagccactcg tagggccgcg cgtcgtcccg caggatctcg gccagcttgc
     2281 gcgcgtcgac cggcacgtcg gtgtggctgt cggccgcgac gtagccgtgg ccagggaagt
     2341 gcttgccgca gttggccatg ccggcctggc gcatgcccag catcaggctc ttggccagca
     2401 gcgtgacgac gcgcgcgtcg cggtggaacg agcggtcgcc gatgacgacg ctgcggccga
     2461 agtgcaggtc cagcactggc gcgaagctca ggtcgacacc gcacgaacgc agctcggcgg
     2521 ccatcacgaa gccggccgcg gtggccgagt cggtggcggc catggcgccg tcgccggcag
     2581 ggccgcggcc atcgtccatc cagcgcttgc cgtacgcgtc catcgcgggc aggtgcgtga
     2641 agccgtcggt gcggaaacgc tgcacgcggc cgccctcgtg gtcgacgcac acgagcaggt
     2701 cgggacggat cgccttcatc tcggccacca gctcggtcac ctgctggcgg ttgacccagt
     2761 tgcggccgaa caggatcgcg ccgcccgtca gcggatgcgc gaggcggcgc cggtcgtcgg
     2821 cggtcagctg ggtgccggcg acgtcgagga tgacgggggc gtgcgcctcg aagggcgcgg
     2881 cgtcggcctt ctgcagttgt tcgacgacgc gcgcctcgcc ggacggcagg gacttgttgg
     2941 cgttcttctt ggtcttgcgg gccatgggat cagctctggg tttgaacgcc ggcgtcatcg
     3001 gccggccggg tctcgaccac cacgaaggag gccacgtagt cggattcgtc ggtgacggtg
     3061 acgtgcgcga cgaggttgcg cgcctcgaac caggccgcca gttcgccgtg caggcggatg
     3121 aacggcttgc ccgagcgctc gttgaggatc tcgcagctgc gccaggtcat gggcatgtgc
     3181 aggcccatgc cgacggcctt ggagaacgcc tccttggcgg agaagcgcgt cgcgaggaag
     3241 cggatgccgc ggccctcgac cttggcgcgc cgcgcatgga acaccttgat ctcgtcgggg
     3301 cccagcacct tctcggcgaa gcggtcgccc ttgcgctgga gcgtcgcggt gatgcggcgc
     3361 acgtcgcaca ggtcggtgcc gatgccgaag atcacgccag cgtctccgcg gattcggcgt
     3421 cgacgatcgc gcgcaggtag cggtgcacgg tgtcggccat gccgaactcc agcgcgtcgg
     3481 cgacgagcgc gtggccgatc gacacctccg ccacgccggg caccgcgcgg atgaagtcgg
     3541 tcaggttgac gaggctcagg tcgtggcccg cgttgatgcc caggcccagc gccagcgcgg
     3601 cctgcgcggt ggcggtgaac ctgccgagca cctcggcctg gcgcggcgtg ccatgggcgc
     3661 tggcgtaggt ctcggtgtag agctcgatgc ggtcggcgcc caggccgcgc acggcgtcca
     3721 tcgcctcggg cagcggatcc atgaacaggc tcacgcgcac gcccagcgcg tggcattcct
     3781 cgatcagcgg gcgcaggcgc gcggcgtcgc gggcgacgtc ccagccgtgg tcggaggtga
     3841 actggtcctc gctgtcgggc acgaaggtgg cctggtgcgg gcgcgcctcg cggatgaagc
     3901 gcatcaggtt ctgggtcggg ttcccctcga tgttgtactc ggcttcgggc cactgctgca
     3961 gcagcttctt gagctcgaac acgtcggccg cgcggatgtg gcgctcgtcc gggcgcggat
     4021 gcacggtgat gc
//
LOCUS       DATCRO010000406        12563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_533326, whole genome shotgun sequence.
ACCESSION   DATCRO010000406 DATCRO010000000
VERSION     DATCRO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533326"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIN75_10980"
     CDS             <1..324
                     /locus_tag="VIN75_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY8974732.1"
                     /translation="VWTALDGALAIGAYRLARQPSTPERNRALGLFAANVAMITGWSK
                     LFFGRHNLGAAAVGSAAIAATATAYVAAAEPVDRPAAAAGLPLVAWVGFATVLSTAIW
                     KMNRK"
     gene            complement(334..747)
                     /locus_tag="VIN75_10985"
     CDS             complement(334..747)
                     /locus_tag="VIN75_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY8974733.1"
                     /translation="MTMTKTLMIVAELAVINWVLIVAASLIKARAWTPSGMKDAMGNR
                     EQAVDCHGFPARTERAARNMLENMVLFTALALVATVGGVTDPHVELGARIFFWARLAY
                     IPIYMVGIPVARTGVWAISIIGMGMIFASIVQALP"
     gene            complement(1018..1356)
                     /locus_tag="VIN75_10990"
     CDS             complement(1018..1356)
                     /locus_tag="VIN75_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974734.1"
                     /translation="MPAGRPPRQSHHVYVVLLDDRVWNEPAFRKANPDHALTRPCVYV
                     GMTGLDPDLRFDKHKAGIQSNRFVFKYGLRLLPELYAVYNPMPYEAAREMEVELAIGL
                     REAGYGVWQA"
     gene            complement(1497..1694)
                     /locus_tag="VIN75_10995"
     CDS             complement(1497..1694)
                     /locus_tag="VIN75_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974735.1"
                     /translation="MTKTLIVATLLSSFAAVSFAQGASAPADGASAPAKHAKHHKAHK
                     AHKAASAASNPEASADKKGGQ"
     gene            complement(1850..2434)
                     /locus_tag="VIN75_11000"
     CDS             complement(1850..2434)
                     /locus_tag="VIN75_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY8974736.1"
                     /translation="MTAAAPLELDPATTALVLVDLQQGILPYAKAPYDDAAVMARAVP
                     LAAAFHARRALVVRVKVGFSADGGDVLKAVVDAPNPPGPLPADWLDDPADLPTRPGDI
                     AILKRQWGAFHGTELDLQLRRRGIRTLVLAGIATAVGVESTARVAWELGYDLVFAEDA
                     TSGPDAAMHANSFGKVFPRLGRVRTTADVLAALA"
     gene            2498..3157
                     /locus_tag="VIN75_11005"
     CDS             2498..3157
                     /locus_tag="VIN75_11005"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8974737.1"
                     /translation="MSIATPGPGESAVAAGGAAAARAPRRARGQLRVDALLAAAAEEF
                     AAKGFDAATMTGIAAQSGSSIGSLYQFFRTKEAVAEALLGMQVDALWVRLDGLAERAA
                     ALAAPDLGHALATCLVDFRADHPSFATLVERPGPPSPLVAGVRRKVRERVEAILARHA
                     PHADRKAVRAMAPVVQHVMKSAVQLRGDLDGSELKAAARELETMLAGYLAARLAPETP
                     R"
     gene            3289..3762
                     /locus_tag="VIN75_11010"
     CDS             3289..3762
                     /locus_tag="VIN75_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8974738.1"
                     /translation="MLLEGSCHCGAVSYTVTAHEPVPYMRCYCSICRKTAGTGGYAIN
                     LGADNRTLKVKGSEHLSVYRARLDNGETSTGQRHFCRHCGTALWLYDPQWPDLVHPHA
                     GSVDTPLSKPPDVTHMMLEFKPDWVDAHVRPADDTFDDYPKETLAEWHRRHRLES"
     gene            complement(3793..5061)
                     /locus_tag="VIN75_11015"
     CDS             complement(3793..5061)
                     /locus_tag="VIN75_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974739.1"
                     /translation="MLRSPVPGSSFHWLLWSDALTLLSLMVGQVALPWWIAQSGGAHD
                     LATFGVVVSAMSFVAMPLLSPLVDRHPKRGLMVVGLVGFGVAASAMAAFASLSHYRLS
                     VVLAIEAVTVLANALILPAVNSIVTELVPPAALSRALGQQQTAQASGRLVGPAIAGSV
                     LAAVGTARTLWLHALLLFGAAALATRLPRSAHVHEAPKAARGWSAEFRVGLRVNWAIP
                     IERGWVLCNFIGAIVMLPCVTMLLPLKVQSLGLSAAWLGWCEAGLSLGMLAGSLGIAQ
                     AWSRLQGRFATRICGAALMGIAFAVAGWTSDRHVLVAMLALAGVCNAAITLVGKTHRM
                     LARPLAFRARMSSAAIMTTQVSQTLGPAIAGVALAHAGVRTVYVAFGVASFVSALGFL
                     LIPGFRAFMALEHDEVDNWYGRTYPQAFEN"
     gene            complement(5124..6014)
                     /locus_tag="VIN75_11020"
     CDS             complement(5124..6014)
                     /locus_tag="VIN75_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974740.1"
                     /translation="MTTLLIALFVAFAVGFSAWRIVVARRAPSRIADTEIAPPEAAPP
                     AADAAPRQRTPEQEAARATARAKAERVIAAEAGRRKAAIQKAQNVARAEAERRAAEAA
                     KAAASLVSTLPASQLPQPERHAPAVAKADTRPAVVERTVPLPRPVPVSPAAPAVATVL
                     KTTEQTLVMLVDDSKMVRVKSSRLLASHGFQVVTANDGIDALKQLENCCPDLIITDVD
                     MPEMDGFGLVAALKGDARTRQTPLVMITSAEDRHREEALRVGVGLVMGKPYDEAALIA
                     HIRSFKFFAKAEEAESLACA"
     gene            complement(6168..6686)
                     /locus_tag="VIN75_11025"
     CDS             complement(6168..6686)
                     /locus_tag="VIN75_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8974741.1"
                     /translation="MPFLGGYPASTLAQVRELIARGELGAYLARRYPERHEVRNDGAL
                     YEHAMALKQRYLRNAPLLSKVHYDNRLQIDQRALGTLTAVSRVQGGQLKAKKEIRVAA
                     IFKDAPAAMLQMIVVHELAHLKEREHDKAFYALCEHMLPDYHQVEFDTRLYLTWRALE
                     AASVTQVTPADT"
     gene            complement(6733..7410)
                     /locus_tag="VIN75_11030"
     CDS             complement(6733..7410)
                     /locus_tag="VIN75_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8974742.1"
                     /translation="MTEGRRFLAAVFDMDGLLIDSERAISEAWIAGAKALGFKLEMAD
                     FLGVVGRAAQQSVPMLVEMLGSEEAMRATAAKARELLPQADGGPVFPLKPGALMLLEA
                     LAAAGIPCGVASSSDVGEIRHRLGHVDVLKHFRSITGGNEVAHGKPDPAIYLLAASRL
                     GVAPAECIAFEDSENGARAAQAAGIRVVIVPDLKHPPQDVAAKAHGVIGSLADAVAHV
                     PHWFPKL"
     gene            complement(7530..8312)
                     /locus_tag="VIN75_11035"
     CDS             complement(7530..8312)
                     /locus_tag="VIN75_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY8974743.1"
                     /translation="MQLKDKVAIITGSASGIGKEIAIEYAREGAKVAIADLSLAAAQA
                     TADEIVKAGGQAMAVAMDVTDEAAVDQGTAAVVERFGGLDVMISNAGIQIIAPIVDFT
                     YADWKKLSAIHTDGAFLTTRAAMRAMIRQGRGGSIIYMGSVHSHLASPLKSAYVTAKH
                     GLLGLARTVAKEGAKDGIRANVICPGFVRTPLVDKQIPEQAQALGISEADVIRNVMLK
                     DTVDGEFTTVQDVAQCAVFLAAFPTNALTGQSITVSHGWHMQ"
     gene            complement(8434..9078)
                     /locus_tag="VIN75_11040"
     CDS             complement(8434..9078)
                     /locus_tag="VIN75_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY8974744.1"
                     /translation="MKLPRWTRDQMAARVARDIPDGSIVNLGIGLPTLIANHLPAGRE
                     VILQSENGIIGMGPAPAPGEEDYDLTNAGKQPVTLLPGGSFFHHADSFAMMRGGHLDI
                     CVLGAFQVSAHGDLANWHTGAEGAIPAVGGAMDLAIGAKRTFVMMEHLTKAGESKLVE
                     TCTYPLTGLACVSRVYTDLAVIDVGPGGARVVDMAEGLAFDRLQAVTAIPLARA"
     gene            complement(9093..9791)
                     /locus_tag="VIN75_11045"
     CDS             complement(9093..9791)
                     /locus_tag="VIN75_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY8974745.1"
                     /translation="MIDKIVETTAAAVADVRDGATVMIGGFGTAGLPDELIAALLDQG
                     ARDLVVVNNNAGNGDAGLAALLGAGRVRKIICSFPRQVDSWHFDRLYREGKIELELVP
                     QGNLAERIRAAGAGIGAFFTPTGYGTILAEGKETREIDGRMQVLEYAIHADLALIKAE
                     RGDRWGNLTYRKTARNFGPIMAMAARCTIATVHEVVELGALDPEAVVTPGVFVQRVVP
                     IARTVTRGAGFKAA"
     gene            10137..10760
                     /locus_tag="VIN75_11050"
     CDS             10137..10760
                     /locus_tag="VIN75_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974746.1"
                     /translation="MPIRSPSRLSALLLAGATAAFADPVVPPPAWPAVKDNPVTLASD
                     PVRPLMLGTLRVSLDGSTLADTRTAIGAGVAQRQGKGTDSLDWLCYTVSDATPVQRLW
                     LTSSELSRGRIDAVVAAELPAGAAASPQCPDLPARFKPVRFDDGLWLGTPSVELRKAL
                     GIPAKTGPHFSSLFQGQTGNLQMVSSTVIEFKGPRAVSLYVAHSTQN"
     gene            11096..11935
                     /locus_tag="VIN75_11055"
     CDS             11096..11935
                     /locus_tag="VIN75_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974747.1"
                     /translation="MIRLHRTAAVALALVAAQGAARAAVAPEAAQELARKSGLWTQLD
                     SLLPQVRAGMDAGLQRSPGQASPEQRAKMLDCAQASFAGEALRPTALDAIVGTLQPQD
                     VAPLMAWYDSPLGRKIAMIEATSSQQTPDPQERLRRGAEALASATASRRASLDAIVGE
                     THSVDLMADTLMGMALAVQQGLASVDPAASGASIGELRSSIGGRRPQLVAHFARISVP
                     AYAFTYLSLGDDELKQYADYLGTPPAMAFSDATTRGVARSLVAGSVALSHCLKAADTP
                     RSP"
     gene            complement(11973..12299)
                     /locus_tag="VIN75_11060"
     CDS             complement(11973..12299)
                     /locus_tag="VIN75_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974748.1"
                     /translation="MTTLAFRTPPGAVSITAGQSKQLGSVDVSKFSKIRVVCDERTGS
                     GTGINVRLTLTEGNELVAQLDTFTLSPHTQLTRVYDVPGTHLTIFADALGGSGTDGID
                     LLVYGA"
BASE COUNT         1792 a         4433 c         4536 g         1802 t
ORIGIN      
        1 gtgtggacgg cgctggacgg cgcgctggcc atcggcgcct accggctcgc gcgccagccg
       61 tcgacgcccg agcgcaatcg cgcgctgggc ctgttcgcgg ccaacgtggc gatgatcacc
      121 ggctggtcca agctgttctt cggccggcac aacctgggcg cagccgccgt cgggtcggcc
      181 gcgatcgccg ccaccgcgac ggcctacgtc gccgccgccg agccggtgga ccggcccgcg
      241 gcggctgccg gcctgccgct ggtcgcgtgg gtgggcttcg ccaccgtgct cagcacggcg
      301 atctggaaga tgaaccgcaa gtgacgggcg ccgtcagggc agcgcctgca cgatgctcgc
      361 gaagatcatg cccatgccga tgatgctgat cgcccacacg ccggtccgcg ccaccgggat
      421 gcccaccatg tagatcggga tgtaggccag gcgcgcccag aagaagatgc gcgcgcccag
      481 ctccacgtgc ggatcggtga cgccgccgac ggtggccacc agtgcgagcg ccgtgaacag
      541 caccatgttc tccagcatgt tcctggcggc ccgctcggtg cgcgccggga agccgtggca
      601 gtcgaccgcc tgctcgcggt tgcccatcgc gtccttcatg ccggacggcg tccaggccct
      661 ggccttgatg aggctggcgg ccacgatcag cacccagttg atcacggcga gttcggccac
      721 gatcatcagc gtcttggtca tcgtcatgtc ggtctgcccg gcagtggaga ggccgccatc
      781 gtagggcagc gcgggcgtcg ggtcttgcct tttgtgcgtc gagacctcgg cctcggggtc
      841 aggtctcgcg tcggggtccg cgtcggggtc aggtcttgaa agttgcgcgc tgcaacgttc
      901 gctacctgca ggcgccctgc cgcgcaactt tcaagacctg accccgatgc ccaagacctg
      961 accccgatgc cgccgacgcg aagcgttcga acgcacgaaa ggcacgaccc gataccccta
     1021 ggcctgccag acgccgtaac ccgcctcgcg caggccgatc gcgagttcga cctccatctc
     1081 ccgggccgcc tcgtagggca tcgggttgta gacggcgtac aactcgggca gcaggcgcag
     1141 gccgtacttg aagacgaagc ggttcgactg gatgccggcc ttgtgcttgt cgaagcgcag
     1201 gtcagggtcc aggccggtca tgcccacgta gacgcaaggg cgcgtcagcg cgtggtcggg
     1261 gttggccttg cggaacgcgg gctcgttcca gacgcggtcg tccagcagca ccacgtagac
     1321 atggtgggat tggcggggcg ggcggccggc gggcatcgtc tgggcaccga ttgtcgatgt
     1381 tggccgcgcc gcgcccgcaa tgcaaaaggg ccgacctgcg tcgacccctt gcgttggccg
     1441 ccagggcggg ccccggcgtg cagcagcgtc atccggcccg caggccggga gacagcttac
     1501 tggccaccct tcttgtcagc cgaggcttcc gggttggacg cagccgaggc cgccttgtgg
     1561 gccttgtgcg ccttgtggtg cttggcgtgc ttggccgggg ccgaagcgcc gtcggccggg
     1621 gccgaagcgc cctgggcgaa cgagaccgcc gcgaacgagg agagcagggt ggcaacgatg
     1681 agggtcttgg tcattttgaa catttccgtt tcagacgaga ttcgttttgg ctccgactct
     1741 ctcgagcagg cccggcatcg gccaggcttg tcaccacaac gggacgccct cgcgcccggt
     1801 tgacaccgcc gcatcgatac tttgcgcggt atccgtcgcc aagcttgcgt caggcgaggg
     1861 cggcgagcac gtcggccgtc gtccggacgc ggcccaggcg cgggaagacc ttgccgaacg
     1921 agttggcgtg catggcggcg tcggggccgc tggtggcgtc ctcggcgaac acgaggtcgt
     1981 agcccagctc ccaggcgacg cgcgcggtcg actcgacgcc cacggcggtg gcgatgccgg
     2041 ccaggacgag ggtgcggatg ccgcgccggc gcagttgcag gtccagctcc gtgccgtgga
     2101 acgcgcccca ctggcgcttg aggatggcga tgtcgcccgg gcgggtgggc aggtcggccg
     2161 ggtcgtccag ccagtcggcc ggcaacgggc cgggcggatt gggggcgtcg acgacggctt
     2221 tcagcacgtc gccgccgtcc gccgagaatc cgaccttcac gcgcaccacc agcgcccggc
     2281 gcgcgtggaa ggcggccgcc agcggcaccg cgcgggccat cacggccgcg tcgtcgtagg
     2341 gggccttcgc atagggaaga atgccttgtt gcaggtcgac cagcaccagc gccgtggtgg
     2401 cagggtccag ttccagggga gcggcagcgg tcatggcggg atccggtatt aacatgagga
     2461 catcctcgca ttcaagttta tgaggaaaga ctcacaaatg tccatcgcca cgcccgggcc
     2521 aggggaatcg gccgtggccg ccggcggcgc ggccgccgcg cgcgccccgc gccgcgcgcg
     2581 cggccagctg cgcgtcgacg cgttgctggc cgcggcggcc gaggagttcg ccgccaaggg
     2641 cttcgacgcc gcgaccatga ccgggatcgc cgcgcaatcg ggctcgtcca tcggctcgct
     2701 gtaccagttc ttccgcacca aggaggccgt ggccgaggcc ttgctgggca tgcaggtcga
     2761 tgcgctgtgg gtgcggctgg acggcctggc cgagcgcgcg gcggcgctgg ccgcgcccga
     2821 cctcggccat gcgctggcga cctgcctcgt cgacttccgc gccgaccacc cgtcgttcgc
     2881 gacgctcgtc gagcgccccg ggccgccgtc gccgctggtg gccggcgtgc ggcgcaaggt
     2941 gcgcgagcgc gtcgaggcga tccttgcgcg ccatgcgccg cacgccgatc gcaaggccgt
     3001 gcgggcgatg gcgccggtcg tccagcacgt gatgaagtcg gcggtgcagc tgcgcggcga
     3061 cctcgacggc tccgagctca aggccgccgc gcgcgagctc gagacgatgc tggccggcta
     3121 cctggcggcc cgcctcgcgc ccgagacgcc gcgctgagcc gggcccgctg ccacgaccgg
     3181 cgcggcgccc tccatccctc gaacgcgcaa cggcgctcgg tacgcgcctt gcccacgcca
     3241 cgcccgtggc gcgcgcgggt gcgcgtgccc gtgacgcgga gagcgacgat gcttctggaa
     3301 ggatcctgtc actgcggcgc ggtgtcgtac acggtgacgg cgcacgagcc ggtgccctac
     3361 atgcgctgct actgctcgat ctgccgcaag accgccggca cgggcggcta cgcgatcaac
     3421 ctcggcgcgg acaaccgcac gctcaaggtc aagggatctg aacacctgtc cgtgtatcgc
     3481 gcgcgactgg acaacggcga gaccagcacc ggccagcgcc atttctgccg gcactgcggc
     3541 acggcgctgt ggttgtacga cccgcaatgg cccgacctgg tgcacccgca cgccggctcg
     3601 gtcgacacgc cgctgagcaa gccgcccgac gtcacgcaca tgatgctgga gttcaagccc
     3661 gactgggtcg acgcccacgt ccgtccggcc gacgacacct tcgacgatta cccgaaggag
     3721 acgctcgcgg agtggcaccg ccgccacagg ctggagagct aggagcctgc acggcggaag
     3781 ccgcgcggac tcctagtttt caaaggcctg cggatacgtc ctgccgtacc agttgtccac
     3841 ctcgtcgtgc tccagcgcca tgaaggcgcg gaagcccggg atcaggagga agccgagcgc
     3901 cgacacgaac gaggcgacgc cgaatgcgac gtagaccgtg cggacgccgg catgggccag
     3961 cgcgacgccc gcgatggccg ggcccagcgt ctgcgacacc tgcgtcgtca tgatcgccgc
     4021 cgatgacatg cgcgcccgga acgcgagcgg gcgcgcgagc atgcggtgcg tcttgcctac
     4081 cagcgtgatc gcggcgttgc acacgcccgc cagcgcgagc atggccacca gcacgtggcg
     4141 atcgctggtc cagccggcca cggcgaacgc gatgcccatc agcgccgcac cgcagatgcg
     4201 cgtcgcgaag cgtccctgca ggcgcgacca cgcctgcgcg atgcccagcg atcccgcgag
     4261 catccccagc gacagcccgg cctcgcacca gcccagccaa gccgcggaca gccccagcga
     4321 ctgcaccttc agcggcaaca gcatcgtcac gcacggcagc atcacgatcg cgccgatgaa
     4381 attgcacagc acccagccgc gttcgatggg gatggcccag ttcacgcgca ggcccacgcg
     4441 gaactcggcg gaccagccgc gcgcggcctt cggcgcctcg tgaacgtgcg cggagcgcgg
     4501 caggcgcgtg gcgagcgcgg cggcgccgaa cagcagcagc gcgtgcagcc acagcgtgcg
     4561 cgcggtgccc accgcggcca gcaccgaccc cgcgatggcc ggcccgacaa gccggccgct
     4621 ggcctgcgcc gtctgctgct ggcccagcgc cctggacagc gccgccggcg gcaccagctc
     4681 ggtcacgatg ctgttgacgg caggcaggat cagcgcgttg gcgagcacgg tgacggcctc
     4741 gatcgcgagc acgaccgaca gccggtagtg cgacagcgag gcgaacgcgg ccatcgcgct
     4801 ggccgcgacg ccgaagccga cgaggcccac gaccatcagg ccgcgcttgg gatggcggtc
     4861 gaccagcggc gacagcagcg gcatcgcgac gaacgacatc gccgacacca ccacgccgaa
     4921 cgtcgcgagg tcgtgcgcgc cgcccgactg cgcgatccac cacggcagcg ccacctggcc
     4981 caccatcagg gacagcaggg tcagcgcatc gctccagagg agccagtgga aggagctgcc
     5041 cggaacgggg ctgcggagca aggacggcag gggcatcccc gccatgctac cggccgccgg
     5101 ccgccggccg gatcagcgtg cgctcaggcg caggccagcg actcggcctc ctcggccttc
     5161 gcgaagaact tgaagctgcg gatgtgggcg atgagcgccg cctcgtcgta gggcttgccc
     5221 atgaccaggc cgacccccac gcgcagcgct tcctcgcggt ggcggtcctc ggccgaggtg
     5281 atcatcacca gcggggtctg gcgcgtgcgc gcgtcgccct tgagcgccgc gacgaggccg
     5341 aagccgtcca tctcgggcat gtcgacgtcg gtgatgatga ggtcggggca gcagttctcg
     5401 agctgcttga gcgcgtcgat gccgtcgttg gcggtgacga cctggaagcc gtgcgaggcc
     5461 agcaggcggc tggacttcac gcgcaccatc ttggagtcgt cgaccagcat gaccagcgtc
     5521 tgctcggtgg tcttgagcac ggtggcgacg gcgggcgcgg ccggggagac cggcacgggg
     5581 cgcggcagcg gcacggtgcg ctcgacgacc gcgggcctgg tgtcggcctt ggcgacggcc
     5641 ggggcgtggc gctcgggctg ggggagctgg ctggcgggca gcgtcgacac gagcgacgcg
     5701 gcggccttgg cggcttccgc ggcgcggcgc tcggcctcgg cgcgggcgac gttctgcgcc
     5761 ttctggatgg cggccttgcg gcgcccggcc tcggcggcga tgacacgctc ggccttggcg
     5821 cgcgcggtgg cgcgggcggc ctcttgctcg ggcgtgcgct gcctgggcgc ggcatcggca
     5881 gcgggcggcg cggcctcggg cggcgcgatc tcggtgtcgg cgatgcggct cggcgcgcgg
     5941 cgcgcgacca cgatgcgcca ggccgagaag ccgacggcga aggcaacgaa gagcgcgatg
     6001 aggagggtgg tcatgggcga cgccggtggt gctgagcgga tgtccaacag gataggggca
     6061 accggccctg ctgatccgtg gaaacaccga ggcaatccga cccacttcgt gaagtcggcc
     6121 acgcgaacgg gaaccgcgcg cgcgcagtgt ccgcgacgcc cctgcagtca ggtgtctgca
     6181 ggtgtgactt gcgtcacgga cgcggcttcg agcgcgcgcc acgtcaggta caggcgcgtg
     6241 tcgaactcga cctggtggta gtcgggcagc atgtgctcgc acagggcgta gaacgccttg
     6301 tcgtgctcgc gttccttcag gtgcgcgagc tcgtgcacga cgatcatctg cagcatggcg
     6361 gccggcgcgt ccttgaagat cgcggccacg cggatctcct tcttggcctt gagctgcccg
     6421 ccctgcacgc gcgacaccgc cgtcagcgtg ccgagcgcgc gctggtcgat ctgcagccgg
     6481 ttgtcgtagt gcaccttgga cagcagcggc gcgttgcgca ggtagcgctg cttgagcgcc
     6541 atcgcgtgct cgtacagcgc gccgtcgttg cgcacctcgt ggcgctcggg gtagcggcgc
     6601 gccaggtacg cgccgagctc gccacgggcg atcagttcgc gcacctgcgc gagcgtcgag
     6661 gcagggtagc cgccgaggaa gggcaggtcg gagcgggcaa tcacgtgcgg attgtcgcag
     6721 cccgccgcgg cttcacagct tcgggaacca gtgcggcacg tgcgcgaccg cgtccgccag
     6781 cgagccgatg acgccgtgcg ccttcgccgc gacgtcttgc ggcggatgct tcaagtcggg
     6841 aacgatcacg acgcggatgc ccgcggcctg cgcggcgcgg gcgccgttct cgctgtcctc
     6901 gaacgcgatg cattcggccg gggccacgcc caggcggctg gccgccagca ggtagatggc
     6961 cggatcgggc ttgccgtgcg ccacctcgtt gccgccggtg atgctgcgaa agtgcttgag
     7021 aacgtcgaca tggcccagcc ggtggcggat ctcgccgacg tccgacgacg acgccacgcc
     7081 gcacgggatg cccgccgcgg ccagcgcctc cagcagcatc agcgcgcccg gcttgagcgg
     7141 gaacaccgga ccgccgtccg cttgaggcag cagttcgcgc gccttggccg ccgtcgcgcg
     7201 catcgcctcc tcgctgccca gcatctcgac gagcatcggc accgactgct gcgcggcgcg
     7261 gcccaccacg ccgaggaagt cggccatctc cagcttgaac ccgagcgcct tcgcgccggc
     7321 gatccacgcc tcggagatgg cgcgctcgga gtcgatcagc aggccgtcca tgtcaaagac
     7381 ggccgcgaga aagcgtcggc cctcagtcag cggagtaccg ctgccagctc cccgagggag
     7441 cgcgcggcct tgctccggag cggccgagcg cggcgcccgc gcgctttcgc cctccgctcg
     7501 atgctcagga cttcctgccg gcattgtcgt cactgcatgt gccagccgtg gctcacggtg
     7561 atggactggc ccgtgagcgc gttcgtcggg aacgccgcca ggaacaccgc gcactgcgcc
     7621 acgtcctgca ccgtcgtgaa ctcgccgtcg accgtgtcct tgagcatcac gttcctgatg
     7681 acgtcggcct cgctgatgcc gagcgcctgc gcctgctcgg ggatctgctt gtcgaccagc
     7741 ggcgtgcgca cgaagcccgg gcagatgacg ttggcgcgga tgccgtcctt ggcgccttcc
     7801 ttggcgacgg tgcgcgccag gcccagcagg ccgtgcttgg cggtgacgta ggccgacttc
     7861 agcggcgagg ccaggtgcga gtgcaccgag cccatgtaga tgatggagcc gccgcggccc
     7921 tgcctgatca tcgcgcgcat cgcggcgcgg gtggtcagga acgcgccgtc ggtgtggatg
     7981 gcgctgagct tcttccagtc ggcgtacgtg aagtcgacga tgggcgcgat gatctggatg
     8041 cccgcgttgc tgatcatcac gtccagcccg ccgaagcgct cgacgacggc ggccgtgccc
     8101 tggtcgacgg cggcctcgtc ggtgacgtcc atcgccacgg ccatcgcctg gccgccggcc
     8161 ttgacgatct cgtcggccgt ggcctgcgcc gccgcgagcg acaggtcggc gatggcgacc
     8221 ttggcgcctt cgcgcgcgta ctcgatcgcg atctccttgc cgatgccgct ggccgagccc
     8281 gtgatgatgg cgaccttgtc cttgagttgc atggggtgct cctgctgatg tgaaatttcc
     8341 ggggcggggt ggcgaagacg tcgcggcgtg tccgctcgat tcgaaggtgt tctaccagac
     8401 tccgctgtca gggccttgac cgccgcgccc gcgtcacgcg cgcgcgagcg ggatcgcggt
     8461 gacggcctgc aaccggtcga aggccaggcc ctcggccatg tcgacgacgc gcgccccgcc
     8521 ggggccgacg tcgatcacgg cgaggtcggt gtagacgcgg ctgacgcagg ccaggcccgt
     8581 cagcgggtag gtgcaggtct cgaccagctt gctctcgccg gccttggtca ggtgttccat
     8641 catcacgaag gtgcgcttgg cgccgatcgc caggtccatc gccccgccga ccgccgggat
     8701 cgcgccctcg gcgccggtgt gccagttggc caggtcgccg tgcgcggaca cctggaacgc
     8761 gccgagcacg cagatgtcca ggtggccgcc gcgcatcatc gcgaagctgt cggcatggtg
     8821 gaagaacgag ccgccgggca gcagcgtcac gggctgcttg ccggcgttgg tgaggtcgta
     8881 gtcctcctcg cccggcgccg gcgcggggcc catgccgatg atgccgttct cgctctgcag
     8941 gatcacctcg cgcccggcgg gcaggtggtt ggcgatcagc gtgggcaggc cgatgcccag
     9001 gttgacgatg ctgccgtcgg ggatgtcccg ggcgacgcgc gcggccatct ggtcgcgcgt
     9061 ccagcggggg agtttcatgg tgtcggtcat ggtcatgcgg ccttgaagcc ggcgccgcgc
     9121 gtgacggtgc gcgcgatcgg caccacgcgc tgcacgaaca cgcccggcgt caccaccgcc
     9181 tcggggtcga gcgcgccgag ttccaccacc tcgtgcaccg tggcgatcgt gcacctggcg
     9241 gccatcgcca tgatcgggcc gaagttgcgc gccgtcttgc ggtaggtaag gttgccccag
     9301 cggtcgccgc gttcggcctt gatcagcgcc aggtcggcgt ggatcgcgta ttcgagcacc
     9361 tgcatcctgc cgtcgatctc gcgcgtctcc ttgccctcgg ccaggatggt gccgtagccg
     9421 gtgggcgtga agaaggcgcc gatgccggcg cccgcggcgc ggatgcgctc ggcaaggttg
     9481 ccctgcggca ccagctcgag ctcgatcttc ccctcgcggt acagccggtc gaagtgccag
     9541 gagtcgacct ggcgcgggaa gctgcagatg atcttgcgca cccggccggc gcccagcagc
     9601 gcggccaggc ccgcgtcgcc gttgccggcg ttgttgttga cgaccaccag gtcgcgcgca
     9661 ccctgatcga gcagcgcggc gatcagttcg tcgggcaggc ccgcggtgcc gaagccgccg
     9721 atcatcaccg tcgcgccgtc ccggacgtcc gccaccgcgg ccgccgtggt ctcaacgatc
     9781 ttgtcgatca tcgcctcttc gccctcgcgc ttgcaggaac agcccgccaa tctaccaaac
     9841 ggggtgcgac ccttcgcggg agtgcggcat cgcgctgacg caggtgtcgc ccacgtgacg
     9901 cagcggccgt cggccccggg ccacgaaacg tgcgaaatga ccgccgaatg gctcgccaag
     9961 tcagtcgtcc gcccgggaaa tggcggacga ttgacgccga cgcgggtcgt ggcggcttat
    10021 ggcaagacag ctggcgcgat tccctgcgag actgctgggc agtgcggggc ccggcccgca
    10081 ccgggcgcac gaagaggcgt ccgagcagac aaacgatctc ggagaccttt cgaaccatgc
    10141 cgatccgctc gccttcccgg ctgtccgcgc tgctgctcgc cggcgccacg gccgcgttcg
    10201 ccgatcccgt cgtgccgccg ccggcctggc cggccgtcaa ggacaacccc gtcacgctcg
    10261 cctccgaccc ggtgcggccg ctgatgttgg gcacgctgcg cgtctcgctg gacggctcga
    10321 cgctggccga cacccgcacc gccatcggtg ccggcgtcgc gcagcgccag ggcaagggca
    10381 cggactcgct ggactggctg tgctacaccg tctccgacgc caccccggtg cagcgcctgt
    10441 ggctgacttc cagcgagctg tcgcgcggcc gcatcgacgc ggtcgtcgcg gcggaactgc
    10501 ccgccggcgc cgcggccagc ccgcagtgcc cggacctgcc ggccaggttc aagccggtgc
    10561 gcttcgacga tggcctgtgg ctgggcacgc ccagcgtgga gctgcgcaag gcgctgggca
    10621 tcccggccaa gactggcccc catttctcga gcctgttcca gggccagacc ggcaacctgc
    10681 agatggtcag ctcgacggtg atcgagttca aggggccacg cgccgtgtcg ctgtacgtgg
    10741 cgcattcgac gcagaactga gcgtcggccg aggtcgatcc tgcgatcgcg cgcaggatga
    10801 cacgccgtgg acggcgccgt gacagcgtcg tggcacgatg ccaacgccag ccgagccggc
    10861 cgcgccagtc cagccgtcat cctgcgcgaa gcgaagcgga ctcgcaggat ccaccttcgc
    10921 gacgatgctg aggttggcag gcgtggatcc cgcgactgca cgcaggatga cacctggtgg
    10981 gcgcgaagcg gcggcagcgc gtcagcacga tccgcgccgg cgggcgcttc acgttccaga
    11041 gacaggccgt ccctatactc ggcgggactt cgcagcagga cctggagacc tcgaaatgat
    11101 tcgtttgcac cggacggccg ccgtggcgct tgcgctggtg gccgcgcagg gcgccgcgcg
    11161 ggccgcggtc gcgcccgagg cggcgcagga gctcgcgcgc aagtccggcc tgtggacgca
    11221 gctcgactcg ctcctgccgc aggtgcgcgc cggcatggac gccggcctgc agcgcagccc
    11281 gggccaagcg agcccggagc agcgcgcgaa gatgctcgac tgcgcccagg cgtccttcgc
    11341 cggcgaagcc ttgcggccga ccgcgctcga cgcgatcgtc ggcacgctgc agccgcagga
    11401 cgtcgccccc ttgatggcct ggtacgacag cccgctgggc cgcaagatcg ccatgatcga
    11461 ggccacgtcg tcgcagcaga cgcccgaccc gcaggaacga cttcgccgcg gcgccgaggc
    11521 gctggccagc gcgacggcga gccggcgcgc gtcgctggac gcgatcgtgg gcgagacgca
    11581 ctcggtcgac ctgatggccg acaccctgat gggcatggcg ctggccgtgc agcagggcct
    11641 ggccagcgtc gatccggcgg cctcgggggc gtcgatcggc gagctgcgct ccagcatcgg
    11701 cggccggcgc ccgcaactgg tggcgcactt cgccaggatc tccgtgccgg cctacgcgtt
    11761 cacctatctg tcgctcggcg acgacgagct caagcagtac gccgactacc tcggcacgcc
    11821 gccggcgatg gcgttcagcg acgccaccac gcgcggcgtg gcgcgctcgc tggtggccgg
    11881 gtcggtggcg ctgagccact gcctgaaggc cgcggacacg cccaggtcgc cctgaggcga
    11941 cgggccgcgg cgagcacgcg gccccgcgtc cttcacgcgc cgtagacgag caggtcgatg
    12001 ccgtcggtgc cgctgccccc gagcgcgtcg gcgaagatcg tcaggtgcgt gccgggcacg
    12061 tcgtacacgc gcgtgagctg cgtgtgcggg gacagcgtga acgtgtccag ctgcgcgacg
    12121 agctcgttgc cctcggtcag cgtcaggcgc acgttgatgc cggtgcccga gccggtgcgc
    12181 tcgtcgcaga cgacgcggat cttggaaaac ttgctgacgt cgacgctgcc cagttgcttg
    12241 gactggcccg ccgtgatcga gacggcgccg ggcggcgtgc ggaatgcgag cgtggtcatg
    12301 gtgacttccc cgtgggttgg ctaggcggtg ccaaatggcg attgcgcgga cgcggacacg
    12361 ggcaccgtcg cctccggcca cgcgcgttcc gcacagtcga ctgcagcgga acgcctcgca
    12421 atctagggcc tgttcacact aaatgcgtgc ccgcgccggc tgcctgcgag gccccaggct
    12481 aggcggacgc gacgcggcgg gctggacgcc cgcaaggagc ggccaacgac gcctggggcc
    12541 tcgcaggcag ccggccctgc ggg
//
LOCUS       DATCRO010000407         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_612115, whole genome shotgun sequence.
ACCESSION   DATCRO010000407 DATCRO010000000
VERSION     DATCRO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612115"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2706
                     /locus_tag="VIN75_11065"
     CDS             <1..2706
                     /locus_tag="VIN75_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974749.1"
                     /translation="AMQWSVRPWVGNKIPFLFFLPAIVAIAARSGRPAGYFATGVGFA
                     SALVWLAPPGHWWVVGATDELSLLIYLILGALLATVGARLRMTSARASAAEQRLVLAG
                     EDTGIGIFDLDLATRTVYLSPALALLCRVDTRDGAVPLDTVMARMPPEVAREARAMVA
                     RKLSERAAGYEREVRLNLPDGRERWLMLRVHIVWSGDRATRLRGACIDITERRAVDDL
                     LGRTQAELGQQVADLHRLHELTSRLLDTRTLTEQLRMILVAVADFHNARRGLLTLFDA
                     GSGTLGLATSIGFTPDAVSRLMQTRGIDGACAICCAQRERTVIEDIDVDDIDPALRAL
                     ASEEGFRAVHATPLIGQDGDVIGAISIHLDAPHVPTERERALADICARKCANFIERAR
                     AHAAYEESQGRFQAVLDASAVPFVILAPVREDASGRVVDFEWTYVNAAAADALNRPAQ
                     AFEGRRVLDVLPHRWASSESFAKYVEVVDQGHTLDFETRAFYDGREQWFRCVASPLRG
                     GVALWFNDITTRKNDERLLQEADRRKDEFLATLAHELRNPLAPIRQAAMISRLPQATE
                     AQKRWSHEVIERQVRHMALLLDDLLDVSRITRGRLALRRGSNALHEMIEAAVETARPL
                     IDSRHHALEVVLPQAAIVMQADPLRVSQIVANLLTNAAKYTDPNGRIRLLAALDADDV
                     VIEVSDNGIGIAPESLPAVFDMFTQLRGTGDRASGLGIGLALTKGLVELHGGTISVRS
                     EGGGHGSVFTVRLPRGDAPEHAPASGDGMEPRAASARRILVADDNRDAAESLAALLQL
                     EGHEVTLAYDGADALAAYERVHPQVCLLDIGMPLRTGNEVAAEIRARRDGPAPTLVAI
                     TGWGQDADRTQALAAGFDHHLTKPVDPAQLLRLIGDVHGGAHARV"
     gene            2855..3703
                     /locus_tag="VIN75_11070"
     CDS             2855..3703
                     /locus_tag="VIN75_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8974750.1"
                     /translation="MDDHDKGLAHDLDTLGRRRVLLGSAGLAAATLLTAWGCGGGSDT
                     ASDTSSSSGASGSSGSSGSSGSTATCAVSAEETQGPYPADGSNTIDGTVSNALALTGI
                     VRSDITTSFGSMSGTAGGVPVTLELTLVNTSSSCAALDGYAIYVWHCSREGVYSLYGA
                     TDQNWLRGVQPTNASGLATFTTIFPGCYSGRMPHIHIEVYRSETTATSYTNKLKTTQL
                     AFPTDVCTTVYTTASGYTSSESNFTQMSFATDNVFSDGTSTEMATVTGNVTDGYTVAL
                     QIGVPV"
     gene            3738..5072
                     /locus_tag="VIN75_11075"
     CDS             3738..5072
                     /locus_tag="VIN75_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974751.1"
                     /translation="MSGWGARLAAHVKALLADTLFKRLFALMWLALVISHVVAFLAVT
                     REGAMGGGRWPTLPSLPPVSFARHADAPASAGDAAPVNAHGPARPDDRSMRPPAGPGD
                     GGPRPWHDGPDGPGGSDGMGPGGPPPSWMPGNPPPMPSNGPMDGPAGNARGGGLPTSA
                     ALLDYGIRFLIIGLAAWFGARWLSAPMRRLQTASRTLGQSLARNDAPPQVDERNGTVE
                     VRETAHVFNEMSRQLSDQVNSRALLVAAISHDLRTPLTRIRMRLEGNEGDPLTARSIA
                     DIHEMDDLIESALEVFRGTSPHEEPAEATDVFALVQALIDDLADLGQPVGVRGEPATA
                     RVRPAGLRRVVSNLVNNALRYGKRADVRVHRAGAAVRIVIEDEGPGIPADQLDAVTRP
                     FYRLETSRSRLTGGSGLGLYIARDLVARQGGTLTLENRAEGGLRATITLPAA"
     gene            complement(5200..5913)
                     /locus_tag="VIN75_11080"
     CDS             complement(5200..5913)
                     /locus_tag="VIN75_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974752.1"
                     /translation="MSLTRCLVVDDDPDLLQSVCDYLRRFGLEAVPASTAAQMRERLA
                     QGGVDVLILDVMLPDGDGLSICAQLRQRPETAALPVLMLTAQGDPHSRVLGLELGADD
                     YVAKPFEPRELVARIKAVLRRRAAPAGAPGGDTTVEFAGWRFDRLKRQLLSPQQVVVP
                     LSSAEYRLLSAFVERPRRVLTRDQLLDATRGQGAVVADRAIDLAVSRLRQKLSAAQDG
                     EQLICTIRGEGYIFDAQVR"
     gene            complement(6058..6213)
                     /locus_tag="VIN75_11085"
     CDS             complement(6058..6213)
                     /locus_tag="VIN75_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HEY8974753.1"
                     /translation="MLGTIVLIVLVLLLLGALPAWPYSRGWGYAPTGIFGLLVVIALI
                     MLLTGRW"
     gene            6392..>7317
                     /locus_tag="VIN75_11090"
     CDS             6392..>7317
                     /locus_tag="VIN75_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974754.1"
                     /translation="MDAVASGTIGHYSFEEKRRRILAIMAASSGNLVEWFDFYVYSFT
                     TIYFAASFFPKTYTTVQQYLYSALVFAIGFLMRPIGGWMFGKIADKRGRKTSLVISVV
                     MMCAGSLMVACLPTYQQIGFAAPLLLLLARMVQGLSVGGEYGATATYLSEVALRGERG
                     FYSSFQYVTLIGGQLLAVLVVFVLQQVLDEAQLKEFGWRIPFLIGAVTAVVALFLRRT
                     LHETAREADLKSSEAGSFATIWRDHRAAFLTVLGYTAGGSLIFYTFTTYMQKYLITTA
                     HMPKTTATNISLVTLFVYMCMQPLFGMLSDRIG"
BASE COUNT         1064 a         2674 c         2590 g          989 t
ORIGIN      
        1 gccatgcagt ggtcggtgcg gccgtgggtt ggtaacaaga tcccgttcct gttcttcctg
       61 ccggccatcg tcgcgatcgc ggcccgctcg ggccgcccgg ccggctactt cgcgaccggc
      121 gtcggcttcg cgagcgcgct ggtgtggctg gcgccgcccg ggcactggtg ggtggtcggc
      181 gccaccgacg aactgtcgct cctcatctac ctgatcctgg gcgcgctgct cgcgacggtg
      241 ggtgcacgcc tgcgcatgac cagtgcccgc gcgtcggccg ccgagcagcg cctggtgctg
      301 gccggcgagg acaccggcat cggcatcttc gacctggacc tggccacccg cacggtctac
      361 ctgtcgccgg cgctcgcgct gctgtgccgc gtcgacaccc gggacggcgc cgtgcccctg
      421 gacaccgtga tggcgcgcat gccgcccgag gtggcgcgcg aggcgcgcgc catggtggcg
      481 cgcaagctgt ccgaacgcgc cgccggctac gagcgcgagg tgcgcctgaa cctgccggac
      541 ggccgcgagc gctggctgat gctgcgcgtg cacatcgtct ggtcgggcga tcgcgcgacc
      601 cggctgcgcg gggcctgcat cgacatcacc gagcgccgcg cggtcgacga cctgctgggg
      661 cgcacccagg ccgaactggg ccagcaggtg gccgacctgc accggctgca cgagctgacc
      721 agccgcctgc tggacacgcg cacgctgacc gagcagctgc gcatgatcct ggtggccgtg
      781 gccgacttcc acaacgctcg ccgcggcctg ctgacgctgt tcgacgccgg atcgggcacg
      841 ctgggcctgg ccaccagcat cggcttcacg ccggacgcgg tgtcgcggct gatgcagacg
      901 cgcggcatcg acggcgcctg cgcgatctgc tgcgcgcagc gcgagcgcac cgtgatcgag
      961 gacatcgacg tcgacgacat cgacccggcg ctgcgcgcgc tcgcgagcga ggaaggcttt
     1021 cgcgccgtgc acgcgacgcc gctgatcggc caggacggcg acgtcatcgg cgcgatctcg
     1081 atccacctgg acgcgccgca cgtgcccacc gagcgcgagc gcgcgctggc cgacatctgc
     1141 gcgcgcaagt gcgccaactt catcgagcgc gcgcgggcgc atgccgcgta cgaggagtcg
     1201 cagggccgct tccaggcggt gctcgacgcg tcggcggtgc ccttcgtcat cctcgcgccg
     1261 gtgcgcgagg acgccagcgg gcgcgtcgtc gacttcgagt ggacgtacgt caacgccgcg
     1321 gcggccgacg cgctcaaccg cccggcgcag gccttcgagg gccggcgcgt gctggacgtg
     1381 ctgccgcacc gctgggccag cagcgagtcg ttcgccaagt acgtggaggt cgtcgaccag
     1441 ggccacacgc tcgacttcga gacgcgcgcg ttctacgacg gccgcgagca atggttccgc
     1501 tgcgtcgcct caccgctgcg cggcggcgtc gcgctgtggt tcaacgacat caccacgcgc
     1561 aagaacgacg agcgcctcct gcaggaagcc gaccgccgca aggacgagtt cctggccacg
     1621 ctggcgcacg agctgcgcaa cccgctcgcg ccgatccgcc aggccgcgat gatctcgcgc
     1681 ctgccgcagg ccaccgaggc gcagaagcgc tggagccacg aggtgatcga gcgccaggtg
     1741 cgccacatgg cgctgctgct ggacgacctg ctggacgtct cgcgcatcac gcgcggccgg
     1801 ctggctctgc gccgcggcag caatgcgctg cacgagatga tcgaggcagc ggtggagacc
     1861 gcgcgcccgc tgatcgactc gcgccaccac gcgctggagg tggtgctgcc gcaggccgcg
     1921 atcgtgatgc aggccgaccc gctgcgcgtc tcgcagatcg tggccaacct gctgaccaac
     1981 gcggccaagt acaccgaccc gaacgggcgc atccggctgc tggccgcgct cgatgcggac
     2041 gacgtcgtca tcgaggtcag cgacaacggc atcggcatcg cgccggaatc gttgccggcg
     2101 gtgttcgaca tgttcacgca gctgcgcggc accggcgacc gcgccagcgg cctggggatc
     2161 ggcctggcgc tgaccaaggg cctcgtcgag ctgcacggcg gcacgatcag cgtgcgcagc
     2221 gagggcggcg gccacggcag cgtgttcacc gtgcgcctgc cgcgcggcga cgcgcccgag
     2281 cacgcgccgg ccagtggcga tggcatggag ccgcgcgcgg cctcggcgcg ccgcatcctg
     2341 gtggccgacg acaaccgcga cgccgccgag agcctggccg ccctgctgca gctcgagggc
     2401 cacgaggtca cgctggccta cgacggcgcc gatgcgctgg ccgcctacga acgcgtgcac
     2461 ccgcaggtct gcctgctgga catcggcatg ccgctgcgca ccggcaacga ggtcgccgcg
     2521 gagatccgcg cccgacgcga cggcccggcg cccacgctcg tggccatcac cggctggggc
     2581 caggacgccg accgcacgca ggcgctggcc gccggcttcg accaccacct gaccaagccc
     2641 gtcgacccgg cgcagctgct gcgcctgatc ggcgacgtgc acggcggcgc gcacgcgcgc
     2701 gtctgagcgt cacgcgcgag cgcgcggcgg cgcgttcggc aacgtgtccg gcacatgtcc
     2761 gcgccgacac caaccgacta aaccgccgac acgtcggctg cgtccaatgg cctcacgcaa
     2821 cagcctcgtt gcggcaccgc catcaaggac agccatggac gatcacgaca agggcctcgc
     2881 ccacgacctc gacaccctcg gacgccgccg cgtgctgctg ggctccgccg gcctcgccgc
     2941 cgccacgctg ctgaccgcct ggggctgcgg cggcggcagc gacaccgcca gcgacacgag
     3001 cagctcgtcc ggcgcgtcgg gctccagcgg ctcgtcgggc tccagcggca gcacggccac
     3061 ctgcgccgtg agcgcggagg agacccaggg gccctatccg gccgacggct ccaacacgat
     3121 cgacggcacg gtgtcgaacg cgctggcgct gaccggcatc gtccgttcgg acatcaccac
     3181 cagcttcggc agcatgagcg gcaccgccgg cggcgtgccg gtcacgctgg agctcacgct
     3241 ggtcaacacg agcagcagct gcgccgcgct ggacggctac gcgatctacg tctggcactg
     3301 cagccgcgag ggcgtgtact cgctgtacgg cgccaccgac cagaactggc tgcgcggcgt
     3361 gcagcccacc aacgccagcg gcctggccac gttcacgacg atcttcccgg gctgctactc
     3421 ggggcgcatg ccgcacatcc acatcgaggt gtaccgcagc gagacgaccg cgacgtcgta
     3481 caccaacaag ctcaagacga cgcagctcgc gttccccacc gacgtctgca cgaccgtcta
     3541 caccacggcc tcgggctaca cctcgagcga gagcaacttc acgcagatga gcttcgcgac
     3601 cgacaacgtg ttctccgacg gcacgtccac cgagatggcc accgtcacgg gcaacgtgac
     3661 cgacggctac acggtggcgc tgcagatcgg cgtcccggtc tgagcgcctg gcacgccgcc
     3721 tgtcgcagaa tgcgcgcatg tccggatggg gagcgcgctt ggccgcgcat gtgaaggcct
     3781 tgctggcgga cacgctgttc aagcggctgt tcgcgttgat gtggctggcg ctcgtgatca
     3841 gccacgtggt cgcgttcctg gccgtcaccc gcgaaggcgc gatgggcggc gggcgctggc
     3901 cgacgctgcc gtcgctgccg ccggtgtcgt tcgcgcgcca tgcggatgcg ccggcgtcgg
     3961 cgggcgacgc ggcccccgtg aacgcgcacg gccctgcgag gccggacgac cgttccatgc
     4021 gaccgcccgc cggccccggc gacggcggcc cgcggccctg gcatgacggg cccgacgggc
     4081 cgggcggctc cgatggcatg ggcccgggcg ggccgccgcc gtcgtggatg cccggcaacc
     4141 cgccgccgat gccttcgaac ggaccgatgg acggcccggc cggcaacgcg cgcggcggcg
     4201 gcctgccgac gtcggccgcg ctgctggact acggcatccg gttcctgatc atcggcctgg
     4261 ccgcgtggtt cggcgcacgc tggctgtcgg cgccgatgcg ccgcctgcag acggcgtcgc
     4321 gcacgctggg gcagtcgctg gcgcgcaacg atgcgccgcc gcaggtcgac gagcgcaacg
     4381 gcaccgtgga ggtgcgcgag acggcgcacg tcttcaacga gatgtcgcgc cagctcagcg
     4441 accaggtcaa ctcgcgcgcg ctgctggtgg cggcgatctc gcacgacctg cgcacgccgc
     4501 tcacgcgcat ccgcatgcgt ctggaaggca acgagggcga tccgctgacg gcgcgctcca
     4561 tcgccgacat ccacgagatg gacgacctca tcgagtccgc gctcgaggtg ttccgcggca
     4621 cgagcccgca cgaggaaccg gccgaggcca ccgacgtgtt cgcgctggtg caggccctga
     4681 tcgacgacct ggccgacctc ggccagcccg tgggcgtgcg cggggagccg gcgacggcgc
     4741 gcgtgcggcc ggcggggctg cggcgcgtcg tgtccaacct cgtcaacaac gcgctgcgct
     4801 acggcaagcg cgccgacgtg cgcgtgcatc gggctggcgc cgcggtgcgc atcgtcatcg
     4861 aggacgaggg gcccggcatc ccagcggacc agctcgatgc cgtgacgagg ccgttctacc
     4921 gcctcgagac ctcgcgcagc cggctcaccg gcggcagcgg cctcgggctg tacatcgcgc
     4981 gcgatctcgt cgcgcgccag ggcggcacgt tgacgctgga gaaccgcgcc gagggcgggc
     5041 tgcgggcgac gatcacgctg ccggcggcct gacgcccggg cccgcctcgt ctgccgcgcg
     5101 gccgcccggt gtcatcctgc gcgcagtcgc aggatccacc ccggccggcc aggccccggc
     5161 tttgccacgc gcgacgccga gcgaccgcga aggcgctcct taccgcacct gggcatcgaa
     5221 gatgtacccc tcgccccgga tcgtgcagat cagctgctcg ccatcctgcg cggccgacag
     5281 cttctggcgc aggcgcgaca ccgccaggtc gatcgcgcga tcggcgacga cggcgccctg
     5341 gccgcgcgtg gcgtccagca gctggtcgcg cgtgagcacc cggcgcgggc gctcgacgaa
     5401 ggcgctgagc aggcggtact cggcgctgga cagcggcacc accacctgct gcggcgacag
     5461 cagctggcgc ttcaggcggt cgaagcgcca acccgcgaat tcgaccgtgg tgtcgccgcc
     5521 gggcgcgccc gcgggcgccg cgcgacgccg cagcacggcc ttgatgcgcg ccaccagctc
     5581 gcgcggctcg aagggcttgg cgacgtagtc gtcggcgccc agctccaggc ccagcacgcg
     5641 gctgtgcggg tcgccctggg cggtcagcat cagcacgggc agcgcggccg tctcgggccg
     5701 ctggcgcagc tgcgcgcaga tcgacaggcc gtcgccatcg ggcagcatca cgtccaggat
     5761 caggacgtcg acgccgccct gcgccagccg ctcgcgcatc tgcgcggcgg tcgacgcggg
     5821 cacggcctcc aagccgaagc gacgcaggta gtcgcagacc gattgcagca ggtcggggtc
     5881 gtcatcgacg acgaggcagc gcgtgaggga catgtcgagg gggcggtcgt tcgcgggtcc
     5941 agcgcgaagt tatcacgccc ttcacacgcc gtcgcggtcg cgcctcgcga gcgcgacgga
     6001 cgaaagaacg ccccctgctg cgtcgcagca agaggcgtcc ctcgggtcgc gctcgggcta
     6061 ccagcggccg gtcagcagca tgatcagggc gatcacgacg agcaggccga agatgccggt
     6121 gggcgcatag ccccagccgc ggctgtacgg ccaggcgggc agcgcgccca gcagcaggag
     6181 gacgaggacg atcaggacga tggtgccaag catggaattg ctccttggat ttggtgggcc
     6241 gcaggtgtgc cgggggatac ggcctggagt cggcaatcca cggaccaggg gcgtccacat
     6301 cgtggcgccg ctacgcgata tccctcacgg cgcggccgcg ccaaagcggc gcatacgatg
     6361 gagaatcgtc cgaacctcaa ggagacaacg catggatgca gtcgcgagcg gaaccatcgg
     6421 ccactactcc ttcgaggaga agcggcgacg catcctggcg atcatggcgg cgtcgtcggg
     6481 caacctggtc gagtggttcg acttctacgt ctactcgttc acgacgatct acttcgcggc
     6541 gtcgttcttt cccaagacct acacgaccgt ccagcaatac ctgtactcgg cgctcgtctt
     6601 cgcgatcggc ttcctcatgc ggccgatcgg cgggtggatg ttcggcaaga tcgccgacaa
     6661 gcgcggccgc aagacgtcgc tggtgatctc ggtggtgatg atgtgcgccg gatcgctgat
     6721 ggtggcctgc ctgcccacct accagcagat cggcttcgcc gccccgctgc tgctgctgct
     6781 ggcgcgcatg gtgcagggac tgtcggtggg cggcgagtac ggcgccaccg ccacctacct
     6841 gagcgaggtg gcgctgcgcg gcgagcgcgg cttctattcc tcgttccagt acgtgacgct
     6901 gatcggcggc cagctgctgg cggtgctggt ggtgttcgtg ctgcagcagg tcctcgacga
     6961 ggcgcagctc aaggagttcg gatggcgcat cccgttcctg atcggcgcgg tcacggcggt
     7021 cgtcgccctg ttcctgcggc gcacgctgca cgagaccgcg cgcgaggccg acctcaagag
     7081 cagcgaggcc ggcagcttcg ccacgatctg gcgcgaccac cgcgcggcct tcctcacggt
     7141 gctgggctac accgccggcg gctcgctgat cttctacacg ttcaccacgt acatgcagaa
     7201 gtacctgatc acgacggccc acatgcccaa gacgaccgcg accaacatct cgctggtgac
     7261 gctgttcgtc tacatgtgca tgcagccgct gttcggcatg ctgtccgacc gcatcgg
//
LOCUS       DATCRO010000408         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_94262, whole genome shotgun sequence.
ACCESSION   DATCRO010000408 DATCRO010000000
VERSION     DATCRO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94262"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VIN75_11095"
     CDS             complement(<1..965)
                     /locus_tag="VIN75_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8974755.1"
                     /translation="MDRRQLLKRGAFFTVIAATGALTACGGDANEPAGTYKFDQGVAS
                     GDPRDTSCVFWTRCVRADGTSAPIDVLLQVSTAADFSAGLMWVRLTASSDWDNTLRAK
                     ITGLAPNTPLYYRFIAGTDVSPVGRARTAPAAGANVAQVRFGWFTCQDWSINHWGAMS
                     LLAQEDLDFLVHVGDYIYETVGASFQVGAAEPAHSALTLPDGTALGSAKYATTLADYR
                     YLYRTYRSDARLRTLHQQWPVIAIWDDHEFSDDCWQDHQTYTNANLQQTARRRAASQA
                     WVEYMPLDFGDVSFDTANAAYDNIRIYRDFQFGNLMHLVMTDERL"
     gene            complement(1247..2089)
                     /locus_tag="VIN75_11100"
     CDS             complement(1247..2089)
                     /locus_tag="VIN75_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974756.1"
                     /translation="MAKPNFHDRSWHEIDWETWGSQFSIVDRASSVGELQNGISTRYM
                     EVQDLLSQLPPHAGRAHALVSGWRMEVNGYGRTIVFSESTRILRRLDDLHGQVTAQLH
                     AARQAAKAARKARQRAHVASGARDAAAHRAAEAHDEFEGMRHSAATARQRNDDVNPGQ
                     QTAAMRWAAVQAQMARECDHCLADSARPVHARQFRAAVDASGDASVEQMLPARDNGMV
                     RIVVVGGRHQFWTTGLPNLENLGIARYAIYRRDGRTRSFEYVAGIRRPEDTAAQREAV
                     ERGG"
     gene            2606..3382
                     /locus_tag="VIN75_11105"
     CDS             2606..3382
                     /locus_tag="VIN75_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974757.1"
                     /translation="MTLTASPPAALRPDTGGNEPGLFESALLKVMALETRVAAVHALS
                     PRFRLVSLRSEALRRVAHVPGDKVQVRVAGMAFRTFTPFRLGDGDDALELLGFMRDAA
                     PAGDWLGAVAPGDRCHVLGPRRSMDLASIDRSTVFVGDETSLGLALALCDTPLGGLDT
                     HFIFEVADVVEVRGVLEAMGRGMLQHASLVERRPDGSHLLDVEASLQRYAGADSFRQY
                     VLSGQSLTIQRLRRVLRSAGARPSQILAKAYWAPGKIALD"
     gene            3399..>3883
                     /locus_tag="VIN75_11110"
     CDS             3399..>3883
                     /locus_tag="VIN75_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974758.1"
                     /translation="MNRIALVEDHERLSTLVRKALATAGIEVDVFDRIASARHAIAET
                     PYGAVVVDRGLPDGDGLSLVCRMREDGNVTPCLMLTARDAVHDRVEGLEAGADDYLAK
                     PFSMEELAARVRALLRRPAAMQSLTPSHGDIRLAPDQGTMSCGEETVTLPSAELQIML
                     CL"
BASE COUNT          498 a         1329 c         1451 g          605 t
ORIGIN      
        1 tacaggcgct cgtcggtcat gaccaggtgc atcaggttgc cgaactggaa gtcgcggtag
       61 atgcggatgt tgtcgtaggc ggcgttggcg gtgtcgaacg agacgtcgcc gaagtccagc
      121 ggcatgtact cgacccaggc ctgcgacgcg gcacggcggc gcgcggtctg ctgcaggttg
      181 gcgttggtgt aggtctggtg gtcctgccag cagtcgtcgc tgaactcgtg gtcgtcccag
      241 atcgcgatca ccggccactg ctggtgcagc gtgcgcaggc gcgcgtcgct gcggtacgtg
      301 cggtacaggt agcggtagtc ggccagcgtc gtggcgtact tggcgctgcc cagggcggtg
      361 ccgtccggca gcgtgagcgc gctgtgcgcg ggctcggcgg cgccgacctg gaacgaggcg
      421 cccacggtct cgtagatgta gtcgcccacg tgcacgagga agtcgagatc ctcctgcgcg
      481 agcagcgaca tcgcgcccca gtggttgatc gaccagtcct ggcaggtgaa ccagccgaac
      541 ctgacctgcg cgacgttggc gccggccgcc ggtgcggtcc tggcgcggcc caccgggctg
      601 acgtccgtgc cggcgatgaa gcggtagtac agcggcgtgt tcggagccag gcccgtgatc
      661 ttggcgcgca gcgtgttgtc ccagtcgctg ctggcggtca ggcgcaccca catcaggccg
      721 gcgctgaagt cggccgcggt cgacacctgc agcagcacgt cgatcggtgc gctcgtgccg
      781 tcggcgcgca cgcagcgcgt ccagaacacg cagctcgtgt cgcgcgggtc gccgctggcc
      841 acgccctggt cgaacttgta ggtgccggcc ggctcgttgg cgtcgccgcc gcacgcggtc
      901 agcgcgccgg tggccgcgat gacggtgaag aaggcgccgc gcttgagtag ttgtcgacgg
      961 tccatggaaa cgctcctgac ggaaattttt gtggcgcgag gatagcgagg ccacatgtca
     1021 gttcaagcgg gacaacacgg gtgtgccggg ccgccgttgc gtcgtcgcat cgcgtcgcgg
     1081 gggccttggt ggatgccctc ttgcggcccg ccggcgcccg gctttctggg cggtttcccc
     1141 tgcggcgacg tcgcacccgc acgacgtggt cgaggcgtgc cgcgagccgt gaaagcgcgt
     1201 gcgcagcggc cctgtcggcc cggcgccatg cgctagggat gagcgtctaa ccgccgcgct
     1261 cgaccgcctc gcgctgcgcg gcggtgtcct cggggcgccg gatgccggcc acgtattcga
     1321 acgagcgcgt gcggccgtcg cggcggtaga tggcgtagcg cgcgatgccc aggttctcga
     1381 ggttgggcag tcccgtcgtc cagaactggt gacggccgcc gacgacgacg atgcgcacca
     1441 tgccgttgtc gcgcgccggc agcatctgct cgacggacgc gtcgcccgag gcatcgaccg
     1501 cggcgcggaa ctgcctggcg tgcaccggcc tcgcgctgtc ggccaggcag tggtcgcatt
     1561 cgcgcgccat ctgcgcctgc acggcggccc agcgcatcgc ggccgtctgc tggccggggt
     1621 tgacgtcgtc gttgcgctgg cgggcggtgg ccgcgctgtg gcgcatgcct tcgaactcgt
     1681 cgtgcgcctc ggccgcacgg tgcgccgctg catcgcgcgc gccgctggcc acgtgcgcgc
     1741 gctggcgtgc cttgcgagcc gccttggcgg cctggcgcgc ggcatgcagc tgcgcggtga
     1801 cctggccgtg caggtcgtcg agccggcgca ggatgcgcgt cgactccgag aacacgatgg
     1861 tgcggccgta gccgttgacc tccatgcgcc agcccgagac cagcgcgtgg gcgcggccgg
     1921 cgtgcggcgg cagctgcgac agcaggtcct gcacttccat gtagcgcgtg gagatgccgt
     1981 tctgcagttc gcccacggag ctcgcgcggt cgacgatgct gaactgggag ccccaggtct
     2041 cccagtcgat ctcgtgccag ctgcggtcgt ggaaattcgg cttggccatg gccttgcggt
     2101 gcggtttctc ggcgtcgtcc caacggcggc gcccgagcct gcatgtcgga tgtcgcccgg
     2161 cctccctcga tgtcggccga gcaggatgct acaggcccgg tgcgatgctt tgaagtccgc
     2221 gcggccggaa gctagggatt tccggaggcg ggcacaccaa aaagcgggga gaaccacgag
     2281 cccggcgcgg gcggacgacg caacgggcgc gacagcggcc accgcgacgc gcagcgcggc
     2341 cggcccgtcg ccgcgagcgg cgaagtcccg ggcgccggcg gggacatcat gcggcagggg
     2401 gtatggctta cgatcggtta cataacttgg cgactgccct ggctccgcat gtcgggaagg
     2461 ccgcggcccc atgtcgtgcg acgcgagcgt cgcctcgatg ccggcccgtg agcggacagg
     2521 gcgtccgtgg ccgagcgcgg taagtcggcg gaaagccgct cccggcgacg atgcggacat
     2581 ccgcccctcg ccccggagtg cctccatgac cttgaccgca tcgcctcccg cagccctgcg
     2641 ccccgacacc ggcggcaacg agcctggcct gttcgagtcg gccctgctca aggtgatggc
     2701 gctcgagacc cgcgtcgccg ccgtgcatgc gctgtcgccg cgtttccggc tggtgtcgct
     2761 gcgcagcgag gcgctgcggc gcgtcgcgca cgtgccgggc gacaaggtgc aggtgcgcgt
     2821 ggccggcatg gcgttccgca cgttcacgcc gttccggctc ggcgatggcg acgacgcgct
     2881 ggagctcctg ggcttcatgc gcgacgccgc gccggccggc gactggctgg gcgcggtggc
     2941 tccgggcgac cgctgccacg tgctgggccc gcgccgttcg atggacctgg cgtcgatcga
     3001 ccgctccacc gtgttcgtcg gcgacgagac ctcgctgggc ctggcgctgg cgctgtgcga
     3061 caccccgctg ggcggcctgg acacgcactt catcttcgaa gtggccgacg tcgtcgaggt
     3121 gcgcggcgtg ctggaggcga tgggccgtgg catgctgcag catgcgtcgc tggtcgagcg
     3181 ccggccggac ggctcgcacc tgctggacgt ggaggcgtcg ctgcagcgct atgcgggcgc
     3241 cgactcgttc cggcagtacg tgctcagtgg ccagtcgctg accatccagc gcctgcgtcg
     3301 cgtgctcagg tcggcgggcg cgcggccgtc gcagatcctc gccaaggcct attgggcgcc
     3361 gggcaagatc gccctcgatt gaacagtccc gatccgacat gaaccgcatc gccctcgtcg
     3421 aagaccacga acgcctgtcc acgctggtgc gcaaggcgct ggccacggcc ggcatcgagg
     3481 tcgacgtgtt cgatcgcatc gccagcgcgc gccacgcgat cgccgagacg ccgtacggcg
     3541 cggtggtcgt cgatcgcggg ctgcccgacg gcgacggcct gtcgctggtg tgccgcatgc
     3601 gcgaggacgg caacgtcacg ccctgcctga tgctgaccgc gcgcgacgcg gtgcacgacc
     3661 gcgtcgaggg cctggaggcc ggcgcggacg actacctcgc caagccgttc tcgatggagg
     3721 agctcgccgc ccgcgtgcgc gcgctgttgc gccggccggc cgccatgcag agcctgacgc
     3781 cgtcgcacgg cgacatccgc ctggcgcccg accagggcac gatgagctgc ggcgaagaga
     3841 cggtgacgct gccgtcggcg gagttgcaga tcatgctgtg cct
//
LOCUS       DATCRO010000409         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_668327, whole genome shotgun sequence.
ACCESSION   DATCRO010000409 DATCRO010000000
VERSION     DATCRO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668327"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VIN75_11115"
     CDS             <1..232
                     /locus_tag="VIN75_11115"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974759.1"
                     /translation="TGSEKDDSRGWLAYQARSFEAFRSYYRYLPKGAFSLEYTFRLNN
                     PGHFGLPQTRVEAMYAPEMFGESPNAAWTVQP"
     gene            214..>2051
                     /gene="pbpC"
                     /locus_tag="VIN75_11120"
     CDS             214..>2051
                     /gene="pbpC"
                     /locus_tag="VIN75_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY8974760.1"
                     /translation="MDGPAVNSRAPRLRLRATLAALALAGSASAQAAIPSFEQVKVGY
                     RSSETVLLDRHGQPVQVVRTDLKARRGGWVPLAQISPALRAAAIASEDQRFPEHAGVD
                     WQAVAGAAIGRTGPGGTRGASTITMQLAGLLDPDLALPKGGRSVGTKIEQAAAALRIE
                     RSWSKDQILEAWLNLVPFRGEQVGVAAMSQSLFQKAPSGLDAEESAIAAALLRAPNAA
                     PAVVGQRACGVLKAQGRGDGCVAAQGLAELVLGHPATPSIDGEQLAPHFARQLLKPGA
                     RSAPATLRTTLDAGAQRRARDALREQLAQLRDRDVEDGAVVVLDNATGDVLAWVGSSG
                     DLSQAAAVDAVVAPRQAGSTLKPFLYEQAIEQRWLTAASVLDDAPTRLHTPSGLYIPQ
                     NYDHDFKGPVSVRTALGSSLNVPAVRTAVMVSPDRFARRLVALGLPIRKAGDFYGYSI
                     ALGSPEVTLLSLTNAYRALANGGTVSAPRLQPADDLPATTRVMAAAPSFIVADILADN
                     DARVPTFGLDNALATRYWSAVKTGTSKDMRDNWCVGFSARYTVGVWVGNASGAPMHAV
                     SGVTGAAPVWRTVMDALQRSDLPGAHPARPGPAGRRRGGPAGGLFQV"
BASE COUNT          262 a          746 c          777 g          266 t
ORIGIN      
        1 caccggcagc gagaaggacg actcgcgggg ctggctcgcg taccaggcgc gcagcttcga
       61 ggccttccgc tcgtactacc gctacctgcc caagggcgcg ttcagtctcg agtacacgtt
      121 ccgcctgaac aacccgggcc acttcggcct gccgcagacg cgtgtcgagg cgatgtacgc
      181 gcccgagatg ttcggcgaat cgcccaacgc ggcatggacg gtccagccgt gaactcgcgc
      241 gcgcctcgcc tgcgcctgcg cgcgacgctg gccgcgctcg ccctggccgg gtcggcgtcc
      301 gcgcaggcgg cgatcccgtc gttcgagcag gtcaaggtgg gctatcgcag ctcggagacg
      361 gtgctgctcg atcgccacgg ccagccggtg caggtcgtgc gcaccgacct caaggcgcgg
      421 cgcggcgggt gggtgccgct ggcgcagatc tcgccggcct tgcgcgcggc cgcgatcgcg
      481 tcggaggacc agcgcttccc ggaacacgcc ggcgtggact ggcaggccgt ggcgggggcc
      541 gcgatcggcc gcaccgggcc cggcggcacg cgcggggcgt cgacgatcac gatgcagctg
      601 gcgggcctgc tcgaccccga cctcgcgctg cccaagggcg gccgctcggt cggcacgaag
      661 atcgagcagg cggcggccgc gctgcgcatc gagcgcagct ggagcaagga ccagatcctc
      721 gaggcctggc tcaacctggt gccgttccgc ggcgagcagg tgggcgtggc ggcgatgtcg
      781 caatcgctgt tccagaaggc gccgtcgggg ctcgacgcgg aggagtcggc gatcgcggcc
      841 gcgctgctgc gggcgccgaa cgccgcgccg gccgtcgtcg gccagcgagc gtgcggcgtc
      901 ctcaaggcgc agggccgcgg cgacggatgc gtggcggcgc agggcttggc ggagctggtg
      961 ctggggcatc cggcgacgcc ttcgatcgac ggcgagcagc tcgcgccgca cttcgcgcgc
     1021 cagctgctca agccgggcgc gaggtccgcg ccggcgacgc tgcgcacgac gctcgacgcc
     1081 ggcgcgcagc gccgcgcgcg cgacgcgctg cgcgagcagc tcgcgcagct gcgcgaccgc
     1141 gacgtggagg acggcgcggt cgtggtgctg gacaacgcca ccggcgacgt gctcgcgtgg
     1201 gtcggctcca gcggcgacct gtcgcaggcg gcggccgtgg atgccgtcgt cgcaccgcgc
     1261 caggccggct ccacgctgaa acccttcctc tacgagcagg cgatcgagca gcgctggctc
     1321 acggcggcct cggtgctgga cgacgcgccg acgcggctgc ataccccgtc gggtttgtac
     1381 attccgcaga actacgacca tgatttcaag ggcccggtca gcgtgcgcac cgcgctcggc
     1441 tcgtcgctga acgtgcccgc ggtgcgcacc gcggtgatgg tgtcgcccga ccgcttcgcg
     1501 cggcggctgg tcgcgctggg cctgccgatc cgcaaggccg gcgacttcta cggctacagc
     1561 atcgcgctcg gctcgcccga ggtgacgctg ctgtcgctga ccaacgccta ccgcgcgctg
     1621 gccaacggcg gcaccgtgag cgcgccccgc ctgcagcccg ccgacgacct gcccgcgacg
     1681 acgcgcgtga tggcggccgc gccgtcgttc atcgtcgccg acatcctggc cgacaacgac
     1741 gcccgcgtgc cgaccttcgg gctggacaac gcgctggcca cgcgctactg gtcggccgtc
     1801 aagacgggca ccagcaagga catgcgcgac aactggtgcg tgggcttctc cgcgcgctac
     1861 acggtcggcg tgtgggtggg caatgcgagc ggcgcgccga tgcatgcggt ctccggcgtg
     1921 acgggcgccg cgccggtctg gcgcacggtg atggacgcgc tgcagcgcag cgacctgccc
     1981 ggggcgcatc cggcacgtcc cggccccgca ggcaggcgac gtggcgggcc ggcgggcggc
     2041 ctgttccagg t
//
LOCUS       DATCRO010000410         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_702097, whole genome shotgun sequence.
ACCESSION   DATCRO010000410 DATCRO010000000
VERSION     DATCRO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702097"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="dctP"
                     /locus_tag="VIN75_11125"
     CDS             <1..571
                     /gene="dctP"
                     /locus_tag="VIN75_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8974761.1"
                     /translation="DNGFHQMCANRPLHHVADFKGLKMRIQSSKVLDAQMRALGAIPQ
                     VMAFSELYQALQSGVVDGTEGTPSNFYTQKIYEVQKHITLSNHGHLAYAAIVNKKFWD
                     GLPADIRTTLDQCMKDATTYANAIAATENVQSLEKIRASGKTTIYQLTPAEALEWKKA
                     LMPVHKEMEGRVGKDAVAAAYKEAGFVPV"
     gene            728..1375
                     /locus_tag="VIN75_11130"
     CDS             728..1375
                     /locus_tag="VIN75_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8974762.1"
                     /translation="MFGRFLDHLEEWLIAFLMGAATLLIFVAVVQRYAAGAHYPTWLQ
                     WLQDALLKINLSWAQELCIYMFIWMAKFGAAYGVRHGTHVGVDTLVRALPPTPARWLT
                     LFGLFGGAVFTVVIGTMGSVLVWDDGMHYALFHALGRNVDDLTEGATTPDLELPVWIE
                     YMCIPVASYLMCFRFLQVMVAFWKTGELPKHEVAHVEGLEDVDNYEPTAQGSIVK"
     gene            1372..2694
                     /locus_tag="VIN75_11135"
     CDS             1372..2694
                     /locus_tag="VIN75_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8974763.1"
                     /translation="MNALIIFTLLVMLMLTGMPVSIALGLTVMGFFFFLSDVPMASVG
                     MKLFEGLNSFPIMAIPFFILAGNFLTNGGAAKRMIRFAATMVGHMYGGLGLAGVLACA
                     LFAAISGSSVATVVAIGSIILPAMVEAGYPKRFSAGVITTSGALGILFPPSINLVIFS
                     IATNGMNATGPDGNPVPTASVGDLFIAGVVPGVVLSVLLGVTTWYRARKYDYPRMPRE
                     TWGARWKAFREAFWGLALVIIIIGGIYSGIFTPTEAAAIAAVYAFFISVFVYKDLKLK
                     DVPKVLRNAAAMSSMLLYIITNAVLFSFLLTSEQIPQAMATWMASQGFGVVVFLFLVN
                     IILLAAGNFMDPSAIVLIMAPILYPVAVKLGVNPVHMGILMAVNMEVGMCHPPVGLNL
                     YVASGITKMGITELTVAVWPWLLTMIGFLLLVTYWPALSLWLPHLLAH"
     gene            2821..4395
                     /locus_tag="VIN75_11140"
     CDS             2821..4395
                     /locus_tag="VIN75_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974764.1"
                     /translation="MRLLSSPSPALLRPVSRLAPVAIACGLALQAGAAHAQFAGDPLA
                     EGSAAPVRSLDARVRLGAEKVRLPGHERMGLVGLTELLNVGGEFWAGPAVYGAATGRR
                     GGFFVPGVEGAWSHPLGDNIAIDAGVFAGGGGGGAAPVGGGLMLRPHVDLVFRLPGFY
                     TGPTYSKVWFPNGQINSNQFGWMLNVDSSFRYRPAEFTGVATDGSATGLGFDHVDATV
                     TFVHPRNAVSTTGTPLASRIGLVGLRAERTVDGPLWAGIETAGAASGGVAGYAEVLGT
                     AGLRWPVAGDRLSAGVRVAAGLAGGGGINTGGGLLVKAAAGTTLRLTDTLGIGAELGV
                     IDSPRHGRYKATMAAVSLNWALDVPQADLGSWVDTRPGTPTRMEFGSGVEHYRAARKT
                     GGSEALDAVVLQVNRFVAPNIYVTGQAHSAFAGGAGAYSEGLFGVGAQLPVTQRLRLG
                     VEALAGAAGGGGVDTGGGAVAQGRAYVDFALTDSLSLRVAGGKIKSVRGSGGLDAAVV
                     DAALVFRFGVDRSNRR"
     gene            4459..5430
                     /locus_tag="VIN75_11145"
     CDS             4459..5430
                     /locus_tag="VIN75_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8974765.1"
                     /translation="MPRSIWKGAIAFGLVHVPVALYPASQEDDIDFDWLDKRTMDPVG
                     YQRINKRTGKPITKENIVKGVKQEDGTYVLMDDDEIRNAYPKTMQTIEIEGYVKAAEI
                     PFVYLEKPYYLEPLPKADKVYALLREAMIADDVIGIARVVMHTKEHLAALMPDGPMLV
                     LNTLRWATEVRKWNELRIPEAGKAATNLKDAELKMAKQLVSELTVKWKADAYHDHFAE
                     AIKNLIKAKVEAGATHEVTPIEAPVDTSAPSNVVDLTALLKNSLGKRRQAAESELEAE
                     AQADAKPARKAAAKAPAKSAAARKPAARKAAPATRAKGTPSLARKRA"
     gene            5468..>6192
                     /locus_tag="VIN75_11150"
     CDS             5468..>6192
                     /locus_tag="VIN75_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8974766.1"
                     /translation="MPTAPRTSTPRPPARAPRVTRAAAPAPAKRTRAKAPPATDDGAP
                     LARYDAKRDFTITSEPGPVRARTGRQLSFVIQKHDATRLHYDFRLELDGVLLSWAVPK
                     GPSFDPKEKRLAMHVEDHPVAYGTFEGTIPPGQYGAGTVIVWDNGTWEPVGDPRAGLK
                     AGKLVFRLHGRKVAGLWELVKIAKPGERQEPWLLFKKHDEWERPHAEYDVVSALPDSV
                     VAHPLPLLDTPAPVSAPPSGKASA"
BASE COUNT          918 a         2247 c         2115 g          912 t
ORIGIN      
        1 ggacaacggc ttccaccaga tgtgcgccaa ccgcccgctg caccacgtgg ccgacttcaa
       61 gggcctgaag atgcgcatcc agtcgtccaa ggtgctggac gcccagatgc gcgcgctcgg
      121 cgcgatcccg caggtgatgg cgttctccga gctgtaccag gcgctgcagt cgggcgtggt
      181 cgacggcacc gagggcacgc cgtcgaactt ctacacgcag aagatctacg aggtgcagaa
      241 gcacatcacg ctgtccaacc acggccacct cgcgtacgcc gcgatcgtca acaagaagtt
      301 ctgggacggc ctgcccgccg acatccgcac gacgctggac cagtgcatga aggacgcaac
      361 gacgtacgcc aacgcgatcg ccgccaccga gaacgtccag tcgctcgaga agatccgcgc
      421 cagcggcaag accaccatct accagctgac gcccgccgag gcgctggaat ggaagaaggc
      481 gctgatgccg gtgcacaagg aaatggaagg ccgggtcggc aaggacgccg tcgccgccgc
      541 ctacaaggaa gccggcttcg tgccggtctg atcccgcggg ctcgccccgg ccacgccggg
      601 gccgcccccg attgacggcg cgctcgcccg cgtcgtccac tcgtcttcat gcgccggtcg
      661 cccgcgggcg accgcgccgt ttcgtcctgc ccgctcactt tgcgccccgg cgcgagaaag
      721 gcatccgatg ttcggtcgct tcctcgacca cctcgaagag tggctcatcg cgttcctcat
      781 gggcgcggcg acgctgctga tcttcgtggc ggtcgtccag cgctacgccg ctggcgcgca
      841 ctaccccacc tggctgcaat ggctccagga cgcgctcctg aagatcaacc tcagctgggc
      901 ccaggagctg tgcatctaca tgttcatctg gatggccaag ttcggcgccg cgtacggcgt
      961 gcgccacggc acccacgtgg gcgtggacac gctggtgcgc gcgctgccgc ccacgccggc
     1021 gcgctggctc acgctgttcg gcctgttcgg cggcgcggtg ttcacggtgg tgatcggcac
     1081 gatgggctcg gtgctcgtct gggacgacgg catgcactac gcgctgttcc acgcgctggg
     1141 ccgcaacgtc gacgacctca ccgaaggcgc caccacgccc gacctcgagc tgcccgtgtg
     1201 gatcgagtac atgtgcatcc cggtcgcgtc gtacctgatg tgcttccgct tcctgcaggt
     1261 catggtcgct ttctggaaga ccggcgagct gcccaagcac gaggtcgccc acgtcgaggg
     1321 cctggaagac gtcgacaact atgaaccgac ggcccagggg agcatcgtca aatgaatgcg
     1381 ttgatcatct tcacgctgct ggtgatgctg atgctcaccg gcatgccggt gtccatcgcg
     1441 ctcggcctga ccgtgatggg gttcttcttc ttcctgagcg acgtgcccat ggccagcgtc
     1501 gggatgaagc tgttcgaggg gctcaacagc ttcccgatca tggcgatccc gttcttcatc
     1561 ctggcgggca acttcctcac caacgggggg gcggccaaac ggatgatccg gttcgccgcg
     1621 acgatggtgg gccacatgta cggcggcctg gggctggccg gcgtgctggc ctgcgcgctg
     1681 ttcgcggcga tctcgggctc gtcggtggcc accgtggtgg cgatcggctc gatcatcctg
     1741 ccggccatgg tggaggcggg ctatccgaag cgcttctccg cgggcgtgat caccacgtcg
     1801 ggcgcgctcg gcatcctgtt cccaccgtcg atcaacctgg tgatcttctc catcgcgacc
     1861 aacggcatga acgccaccgg gcccgacggc aacccggtgc ccacggcgtc ggtcggcgac
     1921 ctgttcatcg cgggcgtcgt cccgggcgtc gtgctgtcgg tgctgctggg cgtgaccacc
     1981 tggtaccgcg cccgcaagta cgactacccg cgcatgccgc gcgagacctg gggcgcgcgc
     2041 tggaaggcct tccgcgaggc gttctggggc ctggcgctgg tcatcatcat catcggcggc
     2101 atctacagcg gcatcttcac gccgaccgaa gccgcggcca tcgcggcggt ctacgcgttc
     2161 ttcatctcgg tgttcgtcta caaggacctc aagctcaagg acgtgcccaa ggtgctgcgc
     2221 aacgcggccg cgatgagctc gatgctgctg tacatcatca cgaacgcggt gctgttctcg
     2281 ttcctgctga cgtccgagca gatcccgcag gcgatggcga cctggatggc ctcgcagggc
     2341 ttcggcgtgg tggtgttcct gttcctggtc aacatcatcc ttctggcggc cggcaacttc
     2401 atggatccgt cggccatcgt gctgatcatg gcgcccatcc tctacccggt ggccgtcaag
     2461 ctgggcgtca acccggtgca catgggcatc ctgatggccg tcaacatgga ggtcggcatg
     2521 tgccacccgc cggtgggcct gaacctgtac gtggcctcgg gcatcaccaa gatgggcatc
     2581 accgagctca ccgtggccgt gtggccgtgg ctgctcacga tgatcggctt cctgctcctg
     2641 gtgacgtact ggcccgcgct gtcgctctgg ctgccgcacc tgctggcgca ctgaacccga
     2701 tccgtccctg acgaggcccc ggccgcgcga gcgccggggc ctcgttcctt gcgggggaac
     2761 catcggcgcg cgtcagcccg ctaagatcgc gccttccgcc tcgcggccct gcccgtcccc
     2821 atgcgtctgc tgtcctcgcc ctcgcccgcc ctcctccgcc ccgtctcccg tctcgctccg
     2881 gtcgccatcg cctgcggcct cgcgctgcaa gccggggcgg cgcatgccca gttcgcgggc
     2941 gaccccctgg ccgaaggcag cgccgcgccc gtgcgctcgc tggacgcccg cgtgcgcctg
     3001 ggcgccgaga aggtgcgcct gcccggacat gaacgcatgg gcctggtcgg cctgaccgaa
     3061 ctgctcaacg tgggcggcga gttctgggcc ggcccggccg tctacggcgc cgccaccggc
     3121 cgccgcggcg gcttcttcgt gccgggcgtc gagggcgcct ggtcgcaccc gctcggcgac
     3181 aacatcgcga tcgacgccgg cgtgttcgcc ggcggcggcg gcggcggcgc ggcgccggtg
     3241 ggcggcggac tgatgctgcg cccgcacgtc gacctcgtct tccgcctgcc gggcttctac
     3301 acgggcccga cgtactcgaa ggtctggttc cccaacggcc agatcaactc caaccagttc
     3361 ggctggatgc tcaacgtcga ctcgtcgttc cgctaccgcc ccgccgagtt caccggcgtg
     3421 gccacggacg gcagcgccac cgggctgggc ttcgaccatg tcgacgcgac cgtgacgttc
     3481 gtgcacccgc gcaacgccgt ctcgacgacg ggcaccccgc tggccagccg catcgggctg
     3541 gtgggcctgc gcgcggaacg caccgtcgac ggaccgcttt gggccggcat cgagaccgcc
     3601 ggcgcggcct ccggcggggt cgccggctac gccgaggtgc tgggcacggc gggcctgcgc
     3661 tggccggtgg cgggcgaccg cctgagcgcg ggcgtgcgcg tcgcggccgg cctggccggc
     3721 ggcggcggca tcaacaccgg cggcggcctg ctggtcaagg ccgcggccgg cacgacgctg
     3781 cgcctgaccg acacgctggg gatcggcgcc gagctgggcg tcatcgattc gccgcgccac
     3841 ggccgctaca aggccacgat ggccgccgtc tcgctcaact gggcgctgga cgtgccgcag
     3901 gccgacctcg gcagctgggt cgacacccgc cccggcacgc cgacccgcat ggagttcggc
     3961 tccggcgtcg agcactaccg cgccgcgcgc aagacgggcg gcagcgaggc gctggacgcc
     4021 gtggtgctcc aggtcaaccg cttcgtcgca ccgaacatct acgtcaccgg ccaggcgcac
     4081 agcgcgttcg ccggcggcgc gggcgcgtac tcggaaggcc tgttcggcgt gggcgcgcaa
     4141 ctgcccgtga cgcagcggct gcgcctgggc gtcgaggcgc tggcgggcgc tgccggcggc
     4201 ggcggcgtgg acaccggcgg cggcgccgtc gcccaggggc gcgcctacgt ggacttcgcg
     4261 ctgaccgatt cgctgtcgct gcgcgtggcc ggcggcaaga tcaagtcggt gcgcggcagc
     4321 ggcgggctgg acgccgccgt cgtcgacgcg gcgctcgtgt tccgcttcgg cgtcgaccgg
     4381 tcgaacaggc gctgacgcgg gcgcggcgat tgccgctccc ggctcccgcg cgccgtcgcc
     4441 gcgctgtcag gagccgccat gccccgctcg atctggaaag gcgcgatcgc cttcggcctc
     4501 gtccacgtgc cggtggcgct gtatccggcg tcacaggagg acgacatcga cttcgactgg
     4561 ctcgacaagc gcacgatgga tccggtcggc taccagcgca tcaacaagcg caccggcaag
     4621 ccgatcacca aggagaacat cgtcaagggc gtcaagcagg aggacggcac ctacgtgctg
     4681 atggacgacg acgagatccg caacgcctat cccaagacga tgcagaccat cgagatcgag
     4741 ggctacgtca aggccgccga gatcccgttc gtctacctcg agaagccgta ctacctcgag
     4801 ccgctgccca aggccgacaa ggtctacgcg ctgctgcgcg aggcgatgat cgccgacgac
     4861 gtgatcggca tcgcacgcgt ggtgatgcac accaaggaac acctggccgc gctgatgccc
     4921 gacggcccga tgctcgtgct caacacgctg cgctgggcca ccgaggtgcg caagtggaac
     4981 gagttgcgca tccccgaggc gggcaaggcg gccaccaacc tcaaggacgc ggagctgaag
     5041 atggccaagc agctcgtctc cgagctcacg gtcaagtgga aggccgacgc gtaccacgac
     5101 cacttcgccg aggcgatcaa gaacctgatc aaggccaagg tcgaggcggg cgccacgcac
     5161 gaggtgacgc cgatcgaggc gccggtggac acttccgcgc cgtccaacgt ggtcgacctg
     5221 acggcgctgc tcaagaacag cctgggcaag cgccgccagg ccgcggagtc cgagctggag
     5281 gccgaggcgc aggccgacgc caagcccgca cgcaaggccg ccgcgaaggc gcccgcgaag
     5341 tccgcggccg ccaggaagcc ggccgccagg aaggccgcgc cggccacgcg cgccaagggc
     5401 acgccgtcgc tcgcgcgcaa gcgcgcgtga cgccgcccac gggcatggcg aatgccgaat
     5461 ccgcgccatg cccactgccc cgcgcacctc gacccctcgc ccgcccgcgc gcgcgccccg
     5521 cgtgacgcgc gccgccgcgc cggcgcccgc gaagcgcacg cgcgcgaagg cgccgccggc
     5581 caccgatgac ggcgccccgc tggcccgcta cgacgccaag cgcgacttca cgatcacgtc
     5641 cgagcccggc cccgtgcgcg cgcgcaccgg ccggcagctg tcgttcgtca tccagaagca
     5701 cgacgcgacg cggctgcact acgactttcg cctcgagctc gacggcgtcc tgctgtcctg
     5761 ggccgtgccc aaggggccca gcttcgaccc gaaggaaaag cggctggcga tgcacgtgga
     5821 ggaccacccg gtcgcctacg gcacgttcga gggcaccatc ccgccgggcc agtacggcgc
     5881 cggcaccgtc atcgtgtggg acaacggcac ctgggagccc gtgggcgatc cgcgcgccgg
     5941 cctgaaggcc ggcaagctcg tgttccggct gcacggcagg aaggtcgcgg ggctgtggga
     6001 gctcgtgaag atcgccaagc cgggcgagcg ccaggagcca tggctgctgt tcaagaagca
     6061 cgacgagtgg gagcggccgc atgccgagta cgacgtggtc agcgccctgc ccgacagcgt
     6121 cgtcgcgcat ccgctgccgt tgctcgacac gcccgcgccc gtgagcgcgc cgccgtcggg
     6181 caaggccagc gc
//
LOCUS       DATCRO010000411         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_128164, whole genome shotgun sequence.
ACCESSION   DATCRO010000411 DATCRO010000000
VERSION     DATCRO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128164"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VIN75_11155"
     CDS             complement(<1..1866)
                     /locus_tag="VIN75_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8974767.1"
                     /translation="MNDARNSLIAPFDVVGIIASAGGLEAISAVLADLQPGFGAAIVV
                     GQHLGGESGLVDILARRIALPVVWAGDGMILEAGRVHVTPPRLLLEVHPDFSCALSPS
                     LGLARDRPLTVLLQSLADSVGDRALAVVLTGMGSDGAGGARAVKMAGGAVIAQSEETS
                     DHPDMPRAAVIAGAVDLVLPLREIGSTISRVMSGGRFPQPRNEREARHALFGGSGPAR
                     EALREVEWESTEFGQVLGWPASLKAVVAAMLASGFATCIGWGPAFNQLYNDAWIGVLG
                     NKHPAAAAAPSHGTWQELWPSLAGTYRRVLETSQTSYVEDMPLSLKRHGYTEEAYFNL
                     SCSALSDDAGVVCGVMTIAAETTERVLAERRLVALRALTSAVSGAETVTEVCRRAEQL
                     LAEQPRDLPFLLLYRIDGMLQRATLAASAGLAAGSPFAPYAIELQMTPATWPLGRVVR
                     QGEPLLVDDLADRMPALHAGNWPEPPTRAMLLPVRVENEAAGAVLVAGLSPRIAFDRT
                     YEDFVGQLAQQLGASLTDARLRESARERLNRLSELDRTKTEFFSNVSHEFRTPLTLIL
                     APLEDVQTRAAASVELQGELQVAARNAKRLLNLVDTLLDFSQIEAGRLRAQWEMLD"
     gene            2176..3885
                     /locus_tag="VIN75_11160"
     CDS             2176..3885
                     /locus_tag="VIN75_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8974768.1"
                     /translation="MASTSAAVSSMSAAARPMTGEEKKVIFASSLGTVFEWYDFYLYG
                     SLAPIIAAQFFSGLDPSAAFIASLLAFAAGFFVRPFGALVFGRLGDMIGRKYTFLVTI
                     LIMGLSTFIVGVLPSYKAIGVAAPIILIALRMLQGLALGGEYGGAATYVAEHSPRGKR
                     GAYTSWIQTTATLGLFLSLLVIIGVKSAVGDDAFKENWWRAPFIVSILLLAVSVWIRL
                     SMNESPAFKKMKEEGKTSKAPLTESFTKWGNLKIVLLALFGLVAGQGVVWYTGQFYAL
                     FFLTSSLKVDATTAQYMIAAALALGTPFFIVFGTLSDKIGRKPIILAGMVLACATYFP
                     IFHAIAGAANPELAAAQASAQVTVKADPATCSFQGSPVAREVDFTSPCDIAKRALTAN
                     SASYANEALPAGSPTVVTVGDKTLTPPTGALMAGGYKFDEASGKAIGAFKKEVAETLK
                     GAGYPAKAKPIAAFSGQWFTVVGLLWILMIYVTMVYGPIAAMLVELFPTRIRYTSMSL
                     PYHIGNGWFGGLLPSVTFAMVAQNGNMFYGLWYPFWIAAVCFVVGLLFVRETKDVDIY
                     ARD"
BASE COUNT          637 a         1530 c         1475 g          656 t
ORIGIN      
        1 gtccagcatc tcccattgcg cgcgcagccg gcccgcctcg atctgcgaga agtccagcag
       61 cgtgtcgacg aggttgagca ggcgcttggc gttgcgcgcc gccacctgca gttcgccctg
      121 cagctcgacg ctcgcggcag cacgcgtctg cacgtcctcc agcggcgcga ggatcagcgt
      181 gagcggcgtg cggaactcgt ggctcacgtt ggagaagaac tcggtcttcg tgcgatccag
      241 ctcggacagc cggttcaggc gctcgcgcgc cgactcgcgc agccgcgcgt cggtgaggct
      301 ggcgcccagt tgctgcgcga gctgcccgac gaagtcctcg tacgtgcgat cgaacgcgat
      361 gcgcgggctc agacccgcca ccagcacggc gccagccgcc tcgttctcca cgcgcaccgg
      421 cagcagcatc gcgcgcgtcg gcggctcggg ccagttgccc gcgtgcagcg ccggcatccg
      481 gtcggccagg tcgtccacga gcagcggctc gccctggcgc acgacacggc ccagcggcca
      541 cgtcgcgggc gtcatctgca gctcgatcgc atacggcgcg aacggcgaac ccgcggccag
      601 gcccgccgag gccgccagcg tcgcacgctg cagcatgccg tcgatgcggt acagcagcag
      661 gaagggcagg tcgcgcggct gctcggccag caactgctcc gcgcgacggc agacctcggt
      721 gaccgtctcg gcgccggaga ccgccgacgt cagcgcgcgc agcgccacga gacggcgctc
      781 cgccagcacg cgctcggtcg tctccgcagc gatcgtcatc acgccgcaca ccacgccggc
      841 gtcgtcggac aacgcgctgc acgacaggtt gaagtacgcc tcctcggtgt agccgtgacg
      901 cttgagcgac agcggcatgt cctcgacata gctcgtctgc gaggtctcga gcacgcgccg
      961 ataggtgccg gccagcgacg gccacagttc ctgccaggtg ccgtgcgacg gtgcggcggc
     1021 ggcggccggg tgcttgttgc ccagcacgcc gatccaggcg tcgttgtaga gctggttgaa
     1081 cgccgggccc cacccgatgc aggtggcaaa gccgctggcc agcatcgcgg ccacgacggc
     1141 cttgagcgac gcgggccagc ccaggacctg gccgaactcg gtcgactccc actcgacctc
     1201 gcgcaaggcc tcgcgcgcgg gcccggagcc gccgaacagt gcgtggcgcg cctcgcgctc
     1261 gttgcgaggt tgcgggaagc ggccgccgct catcacgcgc gagatcgtgc tgccgatctc
     1321 gcgcagcggc agcacgaggt cgaccgcgcc ggcgatcacc gccgcgcgtg gcatgtcggg
     1381 gtgatcggag gtctcctcgc tctgcgcgat caccgcgccg ccggccatct tcacggcgcg
     1441 ggcgccgccc gccccgtcgc tgcccatgcc ggtcagcacg accgccaggg cgcggtcgcc
     1501 gacgctgtct gccagcgact gcagcagcac ggtgagcggc cggtcgcgcg ccagacccag
     1561 cgatggcgac agcgcgcagc tgaagtccgg atgcacctcc agcagcagcc gtggcggcgt
     1621 cacgtggacg cggcccgctt cgaggatcat gccatcgccc gcccagacga ccggcagcgc
     1681 gatgcgccgc gccaggatgt ccaccagccc gctctcgccg ccgaggtgct ggccgacgac
     1741 gatcgcggct ccgaagccgg gctgcaggtc cgcgagcacc gccgagatcg cctcgagccc
     1801 gcccgcggac gcgatgatgc ccaccacgtc gaagggcgcg atcagggagt tgcgggcgtc
     1861 gttcatgggg ccggtcccgt ctgctggcag gtcgatcgtg cgaccgaatc gaactggcgt
     1921 ccagaccggc gcgcacggcg gcggggattt gccgtcgcgg cacgccggga ctggcggcaa
     1981 tcagcacgcc gtgtgtcgcg atttacccgg acccgtgccg gttttccacc atgaggaacg
     2041 cgccctccgt gtttcccctc ggtttgtaag tttcggagaa ggttcgcgtc agagcgctgt
     2101 cagagggcgg tcccacgatg cgtccaggca ctcccgtcga ggacggtggc cgcattcgac
     2161 aacaggagac ttcccatggc atccaccagc gcagccgtca gctcgatgag cgccgcggcc
     2221 aggccgatga cgggcgagga gaagaaggtc atcttcgcct cgtcgctcgg caccgtcttc
     2281 gagtggtacg acttctatct ctatggatcg ctcgcgccga tcatcgcggc gcagttcttc
     2341 tcgggcctcg acccgtcggc cgcgttcatc gcgtcgctgc tggcctttgc cgcgggcttc
     2401 ttcgtgcggc cgttcggcgc gctggtgttc ggccggctcg gcgacatgat cggccgcaag
     2461 tacaccttcc tggtcaccat cctgatcatg ggcctgtcga cgttcatcgt cggcgtgctg
     2521 cccagctaca aggctatcgg cgtcgccgcg ccgatcatcc tgatcgcgct gcgcatgctg
     2581 cagggcctgg cgctcggcgg cgaatatggc ggcgccgcga cctacgtggc cgagcactcg
     2641 ccgcgcggca agcggggcgc gtacacgtcg tggatccaga ccacggcgac gctgggcctg
     2701 ttcctgtcgc tgctggtgat catcggcgtg aagtcggccg tcggcgacga cgcgttcaag
     2761 gagaactggt ggcgcgcgcc gttcatcgtc tcgatcctgc tgctggccgt cagcgtgtgg
     2821 atccggctgt cgatgaacga gtcgcccgcg ttcaagaaga tgaaggaaga gggcaagacg
     2881 tccaaggcgc cgctgaccga gtccttcacg aagtggggca atctcaagat cgtgctgctc
     2941 gcgctgttcg gcctcgtggc aggccagggc gtcgtctggt acacgggcca gttctacgcg
     3001 ctgttcttcc tgaccagctc gctgaaggtc gatgcgacca ccgcgcagta catgatcgcc
     3061 gccgcgctgg cgctcggcac gccgttcttc atcgtcttcg gcacgctgtc ggacaagatc
     3121 ggccgcaagc ccatcatcct ggccggcatg gtgctcgcct gcgcgaccta cttcccgatc
     3181 ttccacgcca tcgccggcgc cgcgaacccc gaactggccg cggcccaggc cagcgcgcag
     3241 gtcacggtca aggccgaccc ggccacctgc tcgttccagg gcagcccggt ggcgcgcgaa
     3301 gtcgacttca cctcgccgtg cgacatcgcc aagcgggcac tgacggccaa ctccgccagc
     3361 tacgccaacg aggccctgcc ggcgggctcg cccacggtcg tgacggtcgg cgacaagacg
     3421 ctgacgccgc ccacgggcgc gctgatggca ggcggctaca agttcgacga ggcgtcgggc
     3481 aaggcgatcg gcgcgttcaa gaaggaagtc gccgagacgc tcaagggcgc cggctacccg
     3541 gccaaggcca agcccatcgc ggccttcagc ggccagtggt tcacggtcgt cggtctgctg
     3601 tggatcctga tgatctacgt gacgatggtc tatggcccga tcgcggcgat gctggtcgag
     3661 ctgttcccca cccgcatccg ctacacgtcg atgagcctgc cgtaccacat cggcaacggc
     3721 tggttcggcg gcctgctgcc ctcggtgacg ttcgccatgg tcgcccagaa cggcaacatg
     3781 ttctacggcc tgtggtatcc gttctggatc gccgcggtct gcttcgtggt gggcctgctg
     3841 ttcgtcaggg agaccaagga cgtggacatc tacgcccgcg actgacgcag cgccgaagca
     3901 ctctccgaca tgggccctcc gcgggcccat tttcattcgt gcaaatgcag gtgcgcagca
     3961 atttcccgcg ggattgatgc caggccgaca cggcggcacc ggcacgtgca aaatggttcg
     4021 tcaccgcggc gccgaagacc gcgggaccct cgcgcggcgc gccgccgcgc ggtcggcgag
     4081 gccgggcatg cgccgcggcc tccggcggac ggggcggccg gcgacggccc cggtggcggg
     4141 tggggcagga atgacggatc aggaactcga cgacgagctt cgctcgctgg accatgtggc
     4201 cgaggaacgc ggcgccgcgg ccctggacac gattcgcgcc gcggtcgagc gcatgcgcga
     4261 cgacggggac ttcgaccgcc tgatgtgggc gctccccc
//
LOCUS       DATCRO010000412         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1028278, whole genome shotgun sequence.
ACCESSION   DATCRO010000412 DATCRO010000000
VERSION     DATCRO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028278"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..2318)
                     /locus_tag="VIN75_11165"
     CDS             complement(180..2318)
                     /locus_tag="VIN75_11165"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974769.1"
                     /translation="MNDVARLFLETYARGGEVDGGWKFGRALQQAQLDYSEDSLQRID
                     LLLKAIRERAKPQYDEFIADAQGRNFISVLAFYLMAVLSRRTGAEIEWHDARSAREAV
                     SEWANIDETPFTRLVALAPDQGLLFLPLVWLHDRLFSLNLGQVETASDYLRSVAQELE
                     YDLPMAWWNAARLLGVAASTLMSRVAGGHAAMPQLAWQHPRGQRVQTQTLDYDGPDAA
                     ARGEAALADHREQALWRTFAHDTWIHSSGKRVRAISATVQTYGRTPLRLRMAFRYEMP
                     AGDIPFAILDPAVWEFNVPEETFLRLNAAVQRGARSVRWPSGRSWADFWAGRRDVPSL
                     LPAAVGVPPTYRTGEQVRKGDAVLTDGGLYPARVVQLEPGADGHMACVFQDVRGSRRS
                     ATATQLREDMVFVSRSPADHLGACITWLQSHALKPARRETDPNTLGGTAHACFALGNL
                     MWQGLALPKDRAGALSLWQGAANAGNPAAELALAIAYLEGDTVRADVPKGLALLRSAV
                     ARGHAPALARLGQELETGLRVPGDAAQAVDVLTRAAEAGNAHGTYRLAWMHRIGKGVK
                     HDMARCVALLSQAAAGGYAQAQYELAMHCSTGDGVPQDDERAAYWLERAVQGDHGEAM
                     IMLAGKFERGRGVPQDLERAVALYKRAADKRIPAAFYHLGVLTADGRGLDRDLREAMR
                     LMTLAAKDGVHDAREQLEDLSLAMTRESRG"
     gene            complement(2526..3119)
                     /locus_tag="VIN75_11170"
     CDS             complement(2526..3119)
                     /locus_tag="VIN75_11170"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEY8974770.1"
                     /translation="MSETLNETALKQLFLDARTTNKFQSTPITDAKLQELVGLMKWGP
                     TSMNSQPGRIVFVRSPEAKAKLKGALMGGNVDKTMAAPVTAIVAYDTEFHEHLPAQFP
                     AMPGARDMFHGNTSLIETTALRNSSLQGAYLILAARALGLDAGPMSGFDNAAVDKAFF
                     PDGKWKSNFLINLGYGDPSGNWPRGPRLPFETMARIA"
     gene            3291..3872
                     /locus_tag="VIN75_11175"
     CDS             3291..3872
                     /locus_tag="VIN75_11175"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8974771.1"
                     /translation="MRQPVPLDRCTRLLNHGPTVLVSAEHDGRRNVMAAAWSMPLDFT
                     PPKVAVVLDKQTFTRGLLLGSGRFALAVPTVAMAALTDHVGSVSGRDVEKFSHVLATA
                     EPGPATGAPLVEGCVAWLECRRLPEPHIEQAYDLFLGEVVAAWADDRVFRDGRWHFDD
                     ADDSLRSLHHIAGGHFYAIGNEVKYAPTAPGDA"
     gene            complement(3874..>4551)
                     /locus_tag="VIN75_11180"
     CDS             complement(3874..>4551)
                     /locus_tag="VIN75_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974772.1"
                     /translation="APDGLPSVGASDVLVAGSEALEALAGVDRSELADDDQSTWTDAA
                     MRVGASRWAAEPVFAPSAGSLHVATQPGQPGETCVLLVDAKHGADRPLARRCTFGTVW
                     TASARVDAGATAVTLAVQPLATWRELWMFRKGADGWTLQVLPPSTEASGVGYVEFAGW
                     VPGTGQVLAAREVRDPQRASRFLRTYELLDGRTLATQKSADAPSSLKAFNRFQDPAWK
                     RVTVSLR"
BASE COUNT          621 a         1656 c         1636 g          638 t
ORIGIN      
        1 ggtggacgcg gacctcgcag gcacgctggt cgaacgcctg acgcgcgatc cgatgccggg
       61 gctggtgatg gtgcgccacg gtgcggcggc ggtgcaggag ccgttggcgg cctgagctgc
      121 cgtcatcccg cgagaagcga agcggggtcg caggatgagg gctcgatgag accccggagt
      181 cagccgcgcg actcgcgcgt catcgccagc gacagatcct ccagctgctc gcgcgcgtcg
      241 tgcacgccgt ccttggcggc cagggtcatc aggcgcatcg cctcgcgcag gtcgcgatcg
      301 aggccgcggc cgtcggcggt gagcacgccc aggtggtaga aggccgcggg gatgcgcttg
      361 tccgcggcgc gcttgtacag cgccaccgcc cgctccagat cctgcggcac gccgcgcccg
      421 cgttcgaact tgccggcgag catgatcatc gcctcgccgt ggtcgccctg caccgcgcgt
      481 tcgagccagt aggcggcgcg ctcgtcgtcc tggggcacgc cgtcgccggt gctgcaatgc
      541 atcgccagct cgtactgcgc ctgcgcatag ccgcccgccg cggcctgcga cagcagcgcg
      601 acgcagcgcg ccatgtcgtg cttgacgccc ttgccgatgc ggtgcatcca cgcgagccgg
      661 tacgtgccat gcgcgttgcc ggcctcggcc gcgcgcgtga gcacgtcgac ggcctgcgcc
      721 gcatcgcccg gcacgcgcag gcccgtctcg agttcctgcc ccagccgcgc gagcgccggc
      781 gcgtggcccc gggcgaccgc gctgcgcagc agggccaggc ccttgggaac gtcggccctg
      841 acggtgtcgc cctccaggta ggcgatcgcc agcgccagct ccgcggccgg gttgccggcg
      901 ttggccgcgc cctgccacag cgacagcgcg ccggcccggt ccttgggcag cgccaggccc
      961 tgccacatca ggttgccgag cgcgaagcag gcgtgcgccg tgccgccgag ggtgttcgga
     1021 tccgtctcgc ggcgcgcggg cttgagcgca tgcgactgca accacgtgat gcacgcgccg
     1081 aggtggtcgg ccgggctgcg cgagacgaac accatgtcct cgcgcaactg cgtggccgtg
     1141 gcgctgcgcc ggctgccgcg cacgtcctgg aacacgcagg ccatgtggcc gtccgcgccc
     1201 ggctccagct gcaccacgcg cgccggatac aggccgccgt cggtcagcac ggcgtcgccc
     1261 ttgcgcacct gctcgccggt gcgataggtc ggcggcacgc cgaccgcggc gggcagcagc
     1321 gagggcacgt cgcgccggcc cgcccagaag tcggcccagg agcggcccga gggccagcgc
     1381 acgctgcgcg cgccccgctg cacggcggcg ttcaggcgca ggaaggtctc ctcggggacg
     1441 ttgaactccc acaccgccgg atcgaggatc gcgaacggga tgtcgcccgc cggcatctcg
     1501 tagcggaagg ccatgcgcag ccgcagcggc gtgcggccgt aggtctgcac ggtcgccgag
     1561 atcgcgcgca cccgcttgcc cgacgaatgg atccaggtgt catgcgcgaa ggtgcgccac
     1621 agcgcctgct cgcggtggtc ggccagcgcg gcctcgcccc gggcggcggc gtcggggccg
     1681 tcgtagtcca gcgtctgcgt ctggacgcgc tgcccgcgcg gatgctgcca ggccagctgc
     1741 ggcatcgccg cgtgccctcc ggccacgcgc gacatcagcg tcgacgccgc gacgcccagc
     1801 aatcgcgccg cgttccacca cgccatcggc aggtcgtatt cgagctcctg cgcgacgctc
     1861 ctgagatagt cggacgccgt ctcgacctgc cccaggttca gcgagaacag gcggtcgtgc
     1921 agccagacca gcggcaggaa cagcagcccc tggtcgggcg cgagcgccac cagccgcgtg
     1981 aacggggtct cgtcgatgtt ggcccactcg gacacggcct cgcgcgcgga gcgggcgtcg
     2041 tgccattcga tctcggcgcc cgtgcggcgc gacagcacgg ccatcaggta gaaggccagc
     2101 accgagatga agttgcggcc ttgcgcatcg gcaatgaact cgtcgtactg cggcttggcg
     2161 cgttcgcgga tcgccttgag cagcaggtcg atgcgctgca ggctgtcctc ggagtagtcc
     2221 agctgcgcct gctgcagcgc cctgccgaac ttccagccgc catcgacttc gccgccgcgg
     2281 gcgtaggtct ccaggaaaag tcgagcgacg tcgttcaagc ggcctcccag ccgacaccag
     2341 ggctgtcccc tctgcggggg gacggggtga atgtacggcc aaaggccgcg ggcggctgcc
     2401 ggcaccccgt gcgaaagtgc cgtgttgcgc aacggcccgg atgcggcgtc ggggggcccc
     2461 cccggcgcat ccaagagaat gggccgcacg aggcggcccg gtcaagtcgg gaggactgga
     2521 tgccttcagg cgatgcgcgc catcgtctcg aacggcaggc gcggtccgcg cggccagttg
     2581 ccgctcgggt cgccgtagcc caggttgatc aggaagttcg acttccactt gccgtccggg
     2641 aagaaggcct tgtcgacggc ggcgttgtcg aagccgctca tcgggccggc gtccaggccc
     2701 agcgcgcggg ccgcgaggat caggtaagcg ccttgcaggc tgctgttgcg cagagcggtg
     2761 gtctcgatca gcgaggtgtt gccgtggaac atgtcgcgcg cgccgggcat ggcggggaac
     2821 tgggcgggca ggtgctcgtg gaactcggtg tcgtacgcga cgatggccgt gacgggcgcg
     2881 gccatcgtct tgtcgacgtt gccgcccatc agcgcgccct tgagcttggc cttggcctcg
     2941 gggctgcgga cgaacacgat gcggcccggc tggctgttca tcgaggtcgg gccccacttc
     3001 atcaggccga ccagctcctg cagcttcgcg tcagtgatcg gcgtcgactg gaacttgttg
     3061 gtggtgcggg cgtcgaggaa cagctgcttc agggcggttt cgttgagggt ttcggacatg
     3121 gggaactcga tggatgttgg aggggcgcgc cttgaaatgg ctccgcgcgg acagccggat
     3181 gttcgcctgc gtcccgccgt cggccgtgca gcgccgtcga aggccccatt caaattcggt
     3241 caacgcaaca ggtcggcgca agcttcaggc cggcggacga gaatgccgcc atgcgccaac
     3301 ccgtccccct cgaccgctgc acccgactcc tcaaccacgg ccccacggtg ctcgtgagcg
     3361 ccgaacacga cggccgccgc aacgtgatgg ccgccgcatg gtcgatgccg ctcgatttca
     3421 cgccgcccaa ggtggccgtg gtcctcgaca agcagacctt cacccgcggc ctgctgctgg
     3481 gaagcggccg gttcgcgctg gccgtgccga cggtggcgat ggcggcgctg accgaccacg
     3541 tgggcagcgt cagcggccgc gacgtcgaga agttcagcca cgtgctggcc accgccgagc
     3601 ccggcccggc caccggcgcg ccgctggtcg agggctgcgt cgcgtggctg gagtgcaggc
     3661 gcctgcccga gccccacatc gagcaggcct acgacctgtt cctgggcgag gtcgtcgccg
     3721 cctgggccga cgaccgcgtc ttccgcgacg gccgctggca cttcgacgac gccgacgact
     3781 cgctgcgctc gctgcaccac atcgccggcg gccacttcta cgcgatcggc aacgaggtga
     3841 agtacgcccc gacggcgccc ggcgacgctt gagtcagcgc aagctgaccg tcacgcgctt
     3901 ccaggccggg tcctggaacc ggttgaatgc cttcaacgag gatggcgcat ccgccgactt
     3961 ctgcgtggcc agcgtccgcc catcgagcag ctcgtaggtg cgcaggaagc ggctcgcgcg
     4021 ctgcgggtcg cgcacctcgc gcgcggccag cacctggccc gtgcccggca cccagccggc
     4081 gaattccaca taccccaccc cgcttgcctc ggtggacggc ggcagcacct gcagcgtcca
     4141 gccgtcggcg cccttcctga acatccacag ctcgcgccag gtggccagcg gctgcaccgc
     4201 cagcgtcacg gcggtggcgc cggcgtcgac gcgcgccgag gccgtccaga ccgtgccgaa
     4261 ggtgcagcgg cgcgccagcg ggcggtccgc gccgtgcttc gcatcgacca gcaggacgca
     4321 ggtctcgccc ggctggcccg gctgcgtggc cacgtgcagg ctgcctgcgg acggcgcgaa
     4381 caccggctcg gcggcccagc gcgaggcgcc cacgcgcatc gcggcgtcgg tccacgtcga
     4441 ctggtcgtcg tcggcgagct cggacctgtc gacgccggcc agcgcctcca gcgcctcgct
     4501 gccggccacg agcacgtcgc tggcgcccac cgacggcaac ccgtccgggg c
//
LOCUS       DATCRO010000413        10379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_409371, whole genome shotgun sequence.
ACCESSION   DATCRO010000413 DATCRO010000000
VERSION     DATCRO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409371"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VIN75_11185"
     CDS             complement(<1..790)
                     /locus_tag="VIN75_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY8974773.1"
                     /translation="MTAASPSPIGTGIGLRQPHYREVFERRPALAFVEVHSENFFLDG
                     GASMHALERARAAYPVSLHGVGLSLASADRLADEHLAKLERLVDRVEPALVSEHLCWG
                     AVGGVHFNDLLPLPYTREALALLADRVDHVQSVLKRQVLLENLSAYVECSGSQMTETA
                     FLAELARRTGCGLLLDLNNLYVNARNFGFDAAERLDALDGASIGQIHLAGHDVVDDCL
                     VDTHGAVVCDPVWSLYGDAVRRFGPKPTLIEWDTDLPTLDVLLQQ"
     gene            complement(825..1097)
                     /locus_tag="VIN75_11190"
     CDS             complement(825..1097)
                     /locus_tag="VIN75_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY8974774.1"
                     /translation="MDKQVFIRTALASVLALGALGAAHAADANANANKEKCYGIAKAG
                     SNDCASATGSHSCAGTATKDNDKGDWKYVDKGTCDKMGGTTMAPKK"
     gene            1519..2943
                     /locus_tag="VIN75_11195"
     CDS             1519..2943
                     /locus_tag="VIN75_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HEY8974775.1"
                     /translation="MNSVLPVAVACALSLAQATANGATLEIHTTAAGTAERLAASTAR
                     FAPGVQPPEGDVSVFVDPARAYQTLLGIGGAITDASAEVFAKLPPEEQAHLLRAYYDP
                     VDGIGYTLARTTIHSSDFSSGSYTYVAEGDARLETFSIAHDREFRIPLIKRAIAAAGG
                     HLTMFASPWSAPAFMKTNGSMLQGGQLKPEFADAWARYYTLFIRAYEKEGIPIWGITV
                     QNEPMARQRWESMIYSAEEERDFLKNHLGPVMAQAGLGDRKIIVWDHNRDLMTQRARV
                     IFDDPDASKYAWGMGFHWYETWAGGAPMFANVGAVHRAWPDKHLLLTEATVEGYDPAR
                     LQSFANAERYGTALINDFNNGAEGWTDWNILLDEHGGPNHVGNFCFAPVHADTRTGRL
                     TFTPSYFYLGHFSKFIRPGAHRVEAASSRSNLQATAFTNPDGSLATVVMNGTDKAVDY
                     RFFVGKDEAKMSIPAHAIQTLVVR"
     gene            2983..4851
                     /locus_tag="VIN75_11200"
     CDS             2983..4851
                     /locus_tag="VIN75_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8974776.1"
                     /translation="MATGNTVSPRGGGVRTDGKLGRRPRASRAGVRRLRRASRATASP
                     PARFRLSMTTLQDAPRFDPAQAEATAGHLLQQGIAAAGRGASDEAIACMRKAVALRPD
                     LAPAQANLGLLLVAVHRHAEAEAPLRAALGLMPRDAMLRNALGVALEGLQRFDEAEKT
                     YRAALEIDPRLPEAHANLGNCLRRLGRMQQAEAHFVRAIELKPEFAVAHYNLGVLLQE
                     REQYERAIVQYRQALAHRPDYLEALNNLGSCLRTEGFIDEAKATFERVLQLQPLQIEA
                     HCNLAQFKTYRVGDPHVGQLLSQQHRVSALPEEGRARYWFTIGKMLEDTGHHDDSFAA
                     YAQGNRIKRTSTHWDEEAHAALQRRIIATFTRERLAAHAIPPAAQGPTPVFIVGMPRS
                     GTSLLEQVLATLPSVHGAGELTWLPETLHVDEAQGPAGYPQTLAGYSTDDYLQLGRHY
                     VERIRELAPGATHVVDKLPDNFQHLGLIHLMFPGARIIHSMRDPMDSCFSCFSRLFIG
                     NNLGYTYDLAATGRYWVTYHELMRHWRDVLPAGRILDVSYETMVSDFENQARRLVEYL
                     GLPWDARCLGFHENRRIVRTASVAQVRKPIYRTSVARWKHYERHLGALLEIVRDYR"
     gene            complement(4858..7641)
                     /locus_tag="VIN75_11205"
     CDS             complement(4858..7641)
                     /locus_tag="VIN75_11205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8974777.1"
                     /translation="MILPFVVDEQALEQQERALAASGEARLDGLVALAWHLRQRNGRR
                     ATEIAAEARALAAQLALGEASTRGVGLRLALVDAEVGWGLADFDGAQRLVDGVIEVTR
                     DARNLADTAAAADAHWLAANLASDLGDTELRDASLQACEALARRLDDAQRTLMTQACI
                     ARWAVFGDPRLARERWGDAFGDGSGPWPPGVTTWVSDFIGNLAFSDGDNGPAILQRIR
                     VHEDAVATGQLQRAVIALTNIGACFSNLNDHESSLSWMQRGLDEARRLGWPMAIAGAL
                     IQLGGILAPLGRHEAAIELLQEARGMLAIAPKSRNLVIALHYLGEVLNLQGKHAEALE
                     VLAELEERSITLKQTDMLVEVRLARAVAHAGLGRRAQALGLARDALADCRAFNRRTIE
                     IRVLMELARLHTTVPAGDPPAGADDHAAALACLREAVAICDSIEGYQISGELYDALAR
                     EHAHAGEHAVAYEVSRQAAAAREATTSTAALNRSIAMQIGFQTERARADAQLQRSLAS
                     TLQQTTSTLETLGAIGQEITRHLDEHAVFDILRAHVHDLLDATHMSIWTVAADGASIE
                     LAFGIDEGQPMVPGRRVAMDSQQSNVARCARTRGEILRREAPAGFNPNHIPGTLRSAS
                     ALYAPLVVGDSLLGVMSIQSPQADVYAERERLIFRTLCAYGAIGLHNARAYKLLGATL
                     GELQQAQEELARKNALLEQAYLQQQEASFTDPLTQLRNRRFLMAHIEDEIALTLRRFE
                     RAQRQPATDATPDHDLVFYMLDIDLFKSVNDQHGHAAGDAVLVEMARRLRGAIRESDF
                     LIRWGGEEFLLVARATHAGEGAVLAERLRSAVAEQAFEIGDGKALPVTCSIGFASFPY
                     CAAQPRLATWSEVTRIADQALYLAKQDGRNRWAGVEAARPPRDRGHFEQICRDPRSAE
                     DAGDVTILRAWAP"
     gene            complement(7788..9551)
                     /locus_tag="VIN75_11210"
     CDS             complement(7788..9551)
                     /locus_tag="VIN75_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8974778.1"
                     /translation="MSAPERHWFPLTGDDAPDAAAPLVGRRATPIDALSGPTIALAGR
                     IVTMDDAFDVLPDGVLYIAKGRIVATLPRSSRPPSGFETTPVTPTGGTLFPGFIELHN
                     HLAYNVLPLWSPVPRLYQDRGQWPAHPDYRRLISGPMTVIGEYRDDSGRPALLAPLVR
                     YVECKCLMGGCTTSQGIMLNSNAGVQRFFRGILRNVENTAQADLSDADARIADVDAKD
                     ARRFLARLGKEHSCFLLHLSEGITRAGQATSTARKHFLALEVAPDEWAINDTFAGIHA
                     AGLLPQDFTVLHEHGGSMVWSPLSNLLLYGDTARVDAARAAHVPIGLGSDWSPSGSKN
                     LLGEMKVAWLYSHHVLDDLFSPRDIVAMVTRDAAAILKWQPALGTLEAGKHADVCVVD
                     GIDGDPYDALIHAPETAIRLVMIEGVARFGEPALMHALHADGEARTVGGQPRTLFLAD
                     AEGDPDVDAVSLASATSALAEALHTLPALARALETPHAPHAARLVLDAARRPAWTLAL
                     DEIRDDGIDMRPRLPYAGPSDFTGPAIAAPKATSPPLSALLGAIELDPLTVADDPDFL
                     GRIAAEPNVPTAIRDGLALLY"
     gene            complement(9548..10363)
                     /locus_tag="VIN75_11215"
     CDS             complement(9548..10363)
                     /locus_tag="VIN75_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY8974779.1"
                     /translation="MTHAFDPGYIAEPFMGLCEDYPEADVYPDDQFRVEWGPVFHRGR
                     LDGSARVLVIGQDPAQHETIVRRILVGEAGRRLQGLLAKLGITRSYVCVNTWLYSVYG
                     EVKSQTQHSAALNDYRNRWLDALLLGSQVQAVIALGQAADEAWTAWRATASGQAAHVA
                     YAAVTHPTQPESSSRGDKAKLAAATAKLLQNWNAALDVLWPAVTHPDVPTTPVAYGST
                     WADGDRVAIPEFDLPAGLPPWMHERDGWAVRVGANELAKRRDITITVPSGIVA"
BASE COUNT         1526 a         3644 c         3685 g         1524 t
ORIGIN      
        1 cctgctgcag cagcacgtcg agggtgggca ggtcggtgtc ccactcgatc agcgtcggct
       61 tcgggccgaa gcggcgcacg gcgtcgccgt acagcgacca caccgggtcg cagacgacgg
      121 cgccgtgcgt gtcgaccagg cagtcgtcga ccacgtcgtg gccggccagg tggatctggc
      181 cgatgctcgc gccgtccaac gcatcgagtc gttcggccgc gtcgaagccg aagttgcgcg
      241 cgttgacgta caggttgttg aggtccagca gcagcccgca gccggtgcga cgcgccagtt
      301 cggcgaggaa ggccgtctcg gtcatctggc tgccgctgca ctcgacgtag gccgacaggt
      361 tctcgagcag cacctgccgc ttgagcacgg actgcacgtg gtcgacgcga tcggccagca
      421 gcgccagcgc ctcgcgcgtg tacggcagcg gcagcaggtc gttgaagtgc acgccgccga
      481 ccgcgcccca gcacaggtgc tcggaaacca gcgccggctc gacgcgatcg accagccgct
      541 cgagcttggc caggtgctcg tcggccaggc gatccgcgga ggccagcgaa aggccgaccc
      601 cgtgcaggct gaccgggtac gcggcccgcg cacgctcgag cgcgtgcatg gaggcgccgc
      661 cgtccaggaa gaagttctcg ctgtgcacct cgacgaaggc cagcgcgggc cgccgctcga
      721 acacctcgcg gtagtgcggc tggcgcaggc cgatgccggt accgatggga gagggagagg
      781 cggcggtcat gcctggcttt cgtgaggacg cggacgacgc gaggttactt cttcggggcc
      841 atcgtcgtgc cacccatctt gtcgcaggtg cccttgtcga cgtacttcca gtcgcccttg
      901 tcgttgtcct tggtggcggt gccggcgcac gagtgcgagc cggtggcgct ggcgcagtcg
      961 ttgctgccgg ccttggcgat gccgtagcac ttctccttgt tggcattggc gttggcatcg
     1021 gcggcgtggg ccgcgccgag cgcgcccagg gcgagaacgg aggcgagggc ggtgcggatg
     1081 aagacttgct tgtccatgtc gggactcctt gacggttgat gttgatgcgc gctggaagtg
     1141 cagcacctcc gtctgtcggg cgacgtcgcg gttccttacg ggccggccaa agaaaaatta
     1201 gcgatgcgtc ggcaggggac ggcgtcgccc ggaggtgtcg gacatatcct ggatcgggcc
     1261 gtgatgccac ggcgagcatc gatgactgcc ttgcccgcga ccggctggtt cgcgccggtg
     1321 ggcagcgtgc agcgggcaag gatatgccgg acacctcgcc cggcgcccgc gacgacgccg
     1381 cgacgtggcg cctttccgcg cgccaggcac tctggtttga aaccgctttc aaaagcgcgg
     1441 taacatgcgc gagccgagcg tcggcgggcc tgcgggctgc agcggcacgg acgcttcccg
     1501 ccgatctccg gagaccccat gaattccgtc cttcccgtcg ccgtcgcgtg cgcactcagc
     1561 ctggcgcagg ccaccgccaa tggcgccacg ctggagatcc acaccaccgc cgccggcacc
     1621 gccgagcgcc tcgccgccag cacggccaga ttcgcgccgg gcgtgcagcc gcccgagggc
     1681 gacgtgtcgg tgttcgtcga cccggccagg gcctaccaga cgctgctggg gatcggcggg
     1741 gccatcacgg acgccagcgc cgaggtgttc gccaagctgc cgccggagga acaggcgcat
     1801 ctgctgcgcg cgtactacga tccggtcgac ggcatcggct acacgctcgc ccgcaccacc
     1861 atccacagct ccgacttcag cagcggcagc tacacgtacg tggccgaggg cgacgccagg
     1921 ctggagacct tctccatcgc ccacgaccgc gagttccgca tcccgctgat caagcgcgcg
     1981 atcgccgcgg ccggcggcca cctcacgatg ttcgcgagcc cctggagcgc gcccgcgttc
     2041 atgaagacca acggcagcat gctgcagggc ggccagctca agcccgagtt cgccgacgcc
     2101 tgggcgcgct actacacgct gttcatccgc gcctacgaga aggaaggcat ccccatctgg
     2161 ggcatcacgg tgcagaacga gccgatggcc aggcagcgct gggagtcgat gatctacagc
     2221 gccgaggagg agcgcgactt cctgaagaac cacctgggcc cggtgatggc gcaggcgggc
     2281 ctgggcgacc gcaagatcat cgtgtgggac cacaatcgcg acctgatgac gcagcgcgcg
     2341 cgcgtgatct tcgacgaccc ggacgcgtcg aagtacgcgt ggggcatggg cttccactgg
     2401 tacgagacct gggccggcgg ggcgccgatg ttcgccaacg tgggtgccgt tcatcgcgcg
     2461 tggccggaca agcacctgct gctgaccgag gcgaccgtcg agggctacga ccccgccagg
     2521 ctccagtcgt tcgccaacgc cgagcgctac ggcacggcgc tgatcaacga cttcaacaac
     2581 ggcgccgagg gctggaccga ctggaacatc ctgctcgacg agcacggcgg cccgaaccac
     2641 gtcggcaact tctgcttcgc gccggtccat gccgacacgc gcaccgggcg actcacgttc
     2701 acgccgagct acttctatct cggccacttc tcgaagttca tccgcccggg tgcgcaccgc
     2761 gtcgaggcgg cgagcagccg ctcgaacctg caggccaccg cgttcaccaa ccccgacggc
     2821 tcgctcgcca ccgtcgtgat gaacggcacc gacaaggcgg tcgactaccg cttcttcgtc
     2881 ggcaaggacg aggcgaagat gtcgatcccg gcgcacgcga tccagacgct ggtcgtgcgc
     2941 tgaggcgagg cggcactccg ccgcgctcag gcagcgctca gcttggctac cgggaacacg
     3001 gtatctcccc ggggtggtgg cgtgcggacc gatggcaaac tcggacgccg cccgcgtgca
     3061 tcccgcgcgg gcgtccgccg actccggcgc gccagccgcg cgaccgcgag cccgccagcc
     3121 cgattccgcc tttccatgac gacgctgcag gacgctcccc gtttcgatcc cgcccaggcc
     3181 gaggccacgg cgggccacct gttgcagcag gggatcgccg ccgcgggccg cggcgcatcg
     3241 gacgaggcga tcgcgtgcat gcgcaaggca gtcgcgctgc ggcccgacct cgcgccggcg
     3301 caggccaacc tcgggttgct gctggtggcc gtgcaccgcc acgccgaagc ggaagcgccg
     3361 ctgcgcgccg cgctcggcct gatgccgcgc gacgccatgc tgcgcaacgc gctgggcgtc
     3421 gcgctggagg gcctgcaacg cttcgacgag gccgagaaga cctaccgcgc ggcgctcgag
     3481 atcgaccccc gcctgccgga ggcgcacgcg aatctgggca actgcctgcg ccggctcggc
     3541 cggatgcagc aggccgaggc gcatttcgtg cgcgccatcg aactgaaacc cgaatttgcc
     3601 gtggcgcact acaacctggg cgtcctgctg caggagcgcg agcaatacga gcgcgcgatc
     3661 gtccaatacc ggcaggcgct ggcgcaccgg ccggattatc tcgaggcact caacaatctc
     3721 ggcagctgct tgcgcaccga gggtttcatc gacgaggcga aagccacgtt cgagcgcgtc
     3781 ctgcaattgc agccgttgca gatcgaggcg cactgcaatc tcgcgcaatt caagacctat
     3841 cgggtcggcg atccgcatgt cgggcaattg ctgtcgcagc agcaccgggt atcggcgctg
     3901 cccgaggaag gacgcgcgag atactggttc acgatcggca agatgctgga agacaccggc
     3961 catcacgacg attcgttcgc ggcctatgcg caaggcaacc gcatcaagcg caccagcacg
     4021 cattgggacg aggaggcgca cgcggccctg cagcgccgca tcatcgccac cttcacccgt
     4081 gagcgcctgg ccgcccacgc catcccgccc gccgcgcagg ggccgacgcc cgtgttcatc
     4141 gtgggcatgc cgcgctcggg tacgtcgctg ctcgaacagg tgttggcgac gctgcccagc
     4201 gtgcacggcg cgggcgagct gacctggctg cccgaaaccc tgcacgtcga cgaggcgcag
     4261 gggccggccg ggtatccgca aaccctcgcg ggatactcga cggacgatta cctccaactc
     4321 ggccggcact atgtcgagcg catccgtgaa ctcgctccgg gcgccacgca cgtggtcgac
     4381 aagctgccgg acaatttcca gcacctgggc ctgatccacc tgatgttccc gggcgcacga
     4441 atcatccatt cgatgcgcga tccgatggat tcctgtttct cgtgcttctc gcgcctgttc
     4501 atcggcaaca acctcggata cacgtatgac ctggcggcca ccggacgata ctgggtcacg
     4561 tatcacgaat tgatgcggca ctggcgcgac gtcctgcccg ccggacgcat cctggacgtg
     4621 tcgtacgaga ccatggtgtc cgatttcgag aaccaggcgc gccgtctcgt cgagtatctc
     4681 gggctgccct gggatgcgcg ctgcctgggt ttccacgaga accgccgcat cgtgcgcacg
     4741 gccagcgtcg cgcaggttcg caagccgatc taccggacat cggtggcgcg ctggaagcac
     4801 tacgagcgcc acctgggcgc gttgctggag atcgtgcgcg actaccgctg aagcggctca
     4861 gggcgcccag gcgcgcagga tcgtgacgtc gccggcgtcc tcggccgagc gcgggtcgcg
     4921 gcagatctgc tcgaagtgtc cgcggtcgcg cggcggccgc gccgcctcga cgcccgccca
     4981 gcggttgcgg ccgtcctgct tggcgaggta gagcgcctgg tcggcgatgc gcgtcacctc
     5041 cgaccacgtc gccagccgtg gctgcgcggc gcagtacggg aagctggcga agccgatgga
     5101 gcaggtaacc ggcagcgcct tgccgtcgcc gatctcgaac gcctgctccg ccaccgcgct
     5161 gcgcaggcgc tcggccagca cggcgccttc gccggcgtgc gtcgcgcgcg ccaccagcag
     5221 gaattcctcg ccgccccagc ggatcaggaa gtccgattcg cggatcgccc cgcgcaggcg
     5281 gcgcgccatc tccaccagca ccgcgtcgcc cgcggcgtgg ccatgctggt cgttgaccga
     5341 cttgaacagg tcgatgtcca gcatgtagaa gacgaggtcg tggtcgggcg tcgcgtcggt
     5401 ggccggctgg cgctgcgcgc gctcgaagcg gcgcagcgtc agcgcgatct cgtcctcgat
     5461 gtgcgccatc aggaagcgcc ggttgcgcaa ctgcgtcaac gggtcggtga agctggcctc
     5521 ctgctgctgc aggtacgcct gctcgagcag cgcgttcttg cgcgccagct cctcctgcgc
     5581 ctgctgcagc tcgcccaggg tcgcgcccag cagcttgtag gcgcgcgcgt tgtgcaagcc
     5641 gatcgcgccg taggcgcaca acgtgcggaa gatcagccgc tcgcgctcgg cgtagacgtc
     5701 ggcctgcggc gactggatcg acatcacgcc cagcaggctg tcgccgacca ccagcggcgc
     5761 atacagcgcg ctggccgagc gcaacgtgcc ggggatgtgg ttcgggttga agcctgcggg
     5821 cgcctcgcgc cgcaggatct cgccgcgcgt gcgggcgcag cgcgcgacgt tggactgctg
     5881 gctgtccatc gcgacgcggc ggcccggcac catgggctgg ccctcgtcga tgccgaatgc
     5941 cagctcgatg ctggcgccgt cggcggcgac cgtccagatc gacatgtgcg tcgcatccag
     6001 caggtcgtgc acgtgcgcgc gcaggatgtc gaacacggcg tgctcgtcca ggtggcgcgt
     6061 gatctcctgg ccgatcgcgc ccagcgtctc cagcgtcgac gtcgtctgct gcagggtgct
     6121 ggccagcgag cgttgcagct gggcgtcggc gcgcgcgcgc tcggtctgga agccgatctg
     6181 catcgcgatc gagcggttca gcgcggccgt gctggtggtg gcctcgcgcg ccgcggcagc
     6241 ctggcgcgac acctcgtagg cgacggcgtg ctcgccggcg tgcgcgtgct cgcgcgcgag
     6301 ggcgtcgtac agctcgccgg agatctggta gccctcgatc gagtcgcaga tcgccaccgc
     6361 ctcgcgcagg caggcgagcg cggcggcgtg gtcgtcggcg cccgcgggcg ggtcgccggc
     6421 cggcacggtg gtgtgcaggc gcgcgagctc catcagcact cggatctcga tcgtgcggcg
     6481 attgaacgcg cggcagtcgg ccagcgcgtc gcgcgcgagg ccgagtgcct gtgcacgccg
     6541 gcccaggccc gcgtgcgcga ccgcgcgcgc gaggcgcacc tccaccagca tgtcggtctg
     6601 cttgagggtg atcgagcgtt cctcgagctc ggccagcacc tcgagcgcct cggcgtgctt
     6661 gccctgcagg ttcagcacct cgcccaggta gtgcagggcg atcacgaggt tgcgcgactt
     6721 cggcgcgatg gccagcatgc cgcgcgcctc ctgcagcagc tcgatggccg cctcgtgccg
     6781 gcccagcggc gcgaggatgc cgcccagctg gatcagcgcg ccggcgatag ccatcggcca
     6841 gccgaggcgg cgcgcctcgt cgaggccgcg ctgcatccag gaaaggctgc tttcgtggtc
     6901 attgagattg gagaagcacg cgccgatgtt ggtcagcgcg atcaccgcgc gctggagctg
     6961 cccggtggcg accgcgtcct cgtgcacgcg gatgcgctgc aggatggcgg ggccgttgtc
     7021 gccgtcggag aacgcgaggt tgccgatgaa gtcgctgacc caggtcgtga cgccgggcgg
     7081 ccacggcccg ctgccgtcgc cgaacgcgtc gccccagcgc tcgcgcgcga ggcgcggatc
     7141 gccgaacacg gcccagcgcg cgatgcaggc ctgcgtcatc agcgtgcgct gcgcatcgtc
     7201 cagcctgcgg gcgagcgcct cgcaggcctg cagcgacgcg tcacgcagct cggtgtcgcc
     7261 caggtcgctc gccaggttgg cggccagcca gtgggcgtcg gccgcggcgg cggtgtcggc
     7321 caggttgcgc gcgtctcgcg tgacctcgat gacaccatcc accaggcgtt gcgcgccgtc
     7381 gaagtcggcc aggccccagc cgacttcagc gtcgaccagc gccagccgca gtccgacacc
     7441 gcgggtcgag gcctcgccca gcgccagttg agcggccagc gcgcgcgcct cggcagcaat
     7501 ctccgtggcg cggcgcccgt tgcgctggcg caggtgccag gccagcgcga cgagtccgtc
     7561 caggcgtgcc tcgcccgagg cggccagggc gcgctcctgc tgctccagcg cctgctcgtc
     7621 gacgacgaag ggcaggatca cggcaaccac gctgcagggg tgggggactg cattttctga
     7681 ctgtagcgca gtcgtgccgc cggggcgcga ggcgaacagg cgagcgcaga cccggccacg
     7741 gccgcgccgg cgatcacaac gctgcgtcac ccaggacggg cgacggatca gtacaggagc
     7801 gccagtccgt cccggatggc cgtcggcacg ttgggctcgg cggcgatccg gccgaggaag
     7861 tccgggtcgt cggccaccgt caacgggtcg agctcgatcg cgccgagcag ggcggacagc
     7921 ggtggcgacg tcgccttggg cgcggcgatg gccgggcctg tgaagtccga cgggccggca
     7981 tagggcaggc gggggcgcat gtcgatcccg tcgtcccgga tctcgtcgag cgcgagcgtc
     8041 caggctgggc ggcgcgccgc gtccagcacg aggcgggcgg cgtgcggcgc atgcggcgtc
     8101 tccagcgccc gggccagcgc gggcagggtg tgcagcgcct cggcgagcgc gctggtcgcg
     8161 ctggccagcg acacggcgtc gacatccgga tcgccctcgg cgtccgccag gaacagggtt
     8221 cgcggctgtc cgcccacggt cctcgcctcg ccgtcggcgt gcagcgcatg catcagcgcg
     8281 ggctcgccga aacgggcgac gccttcgatc atcaccagcc ggatcgccgt ctcgggcgca
     8341 tggatcaggg cgtcgtacgg atcgccgtcg atgccgtcga cgacgcacac atcggcgtgc
     8401 ttgcccgcct cgagcgtgcc gagggccggc tgccacttga ggatggcggc ggcatcgcgc
     8461 gtgaccatcg cgacgatgtc gcgcgggctg aacaggtcat cgagcacatg gtgcgaatag
     8521 agccaggcga ccttcatctc gcccagcagg ttcttgctgc ccgagggcga ccagtcgctg
     8581 cccaggccga tcggcacgtg cgccgcacgg gcggcatcga cgcgcgcggt gtcgccgtac
     8641 agcagcaggt tgctcagcgg cgaccagacc atcgacccgc cgtgctcgtg cagcaccgtg
     8701 aagtcctgcg gcagcaggcc ggcggcgtgg atgccggcga aggtgtcgtt gatcgcccat
     8761 tcgtcgggcg cgacctccag ggccaggaag tgcttgcgcg ccgtcgacgt cgcctggccc
     8821 gcgcgcgtga tgccttcgct caggtgcagc aggaagcagc tgtgctcctt gccgagccgc
     8881 gccaggaacc tgcgcgcgtc cttggcgtcg acgtcggcga tgcgcgcatc cgcgtccgac
     8941 aggtcggcct gcgcggtgtt ctccacgttg cgcaggatgc cgcggaagaa gcgctgcacg
     9001 ccggcattgc tgttgagcat gatcccttgg ctcgtggtgc agccgcccat caggcacttg
     9061 cattccacgt agcgcaccag cggggcgagg agggcggggc ggccggagtc gtcgcgatac
     9121 tcgccgatga ccgtcatcgg gccgctgatc aggcgacggt agtccggatg cgccggccac
     9181 tggccgcgat cctggtacag cctgggcacc gggctccaca gcggcagcac gttgtaggcg
     9241 aggtggttgt gcagctcgat gaagccggga aacagggtgc cgcccgtcgg cgtgacgggc
     9301 gtcgtctcga acccggacgg cggtcgtgag ctgcgcggca gcgtcgcgac gatgcggccc
     9361 ttggcgatgt agaggacgcc gtcgggcagc acgtcgaagg cgtcgtccat cgtcacgatg
     9421 cggccggcca gcgcgatggt cggccccgac agcgcgtcga tgggcgtcgc gcgtcttccg
     9481 accagcgggg cggccgcgtc gggcgcgtcg tcgcccgtca gcgggaacca gtggcgctcg
     9541 ggggcgctca cgccacgatc ccggacggca ccgtgatcgt gatgtcgcgc cgcttggcca
     9601 gctcgttggc cccgacgcgc accgcccagc cgtcgcgctc gtgcatccac ggcggcaggc
     9661 ccgcgggcag gtcgaattcg gggatggcca cgcggtcgcc gtccgcccac gtgctgccgt
     9721 acgcgaccgg agtggtcgga acgtccggat gcgtgaccgc cggccacagc acgtcgagcg
     9781 cggcgttcca gttctgcagc agcttggccg tcgcggccgc gagcttggcc ttgtcgcccc
     9841 tggacgagct ctcgggctgc gtcggatgcg tgaccgccgc gtacgcgacg tgcgccgcct
     9901 ggccgctggc cgtagcccgc caggccgtcc acgcctcgtc ggccgcctgg ccgagcgcga
     9961 tgacggcctg cacctgcgag ccgagcagca acgcgtcgag ccagcggttg cgatagtcgt
    10021 tgagcgccgc cgaatgctgc gtctgcgact tgacctcgcc gtagacgctg taaagccacg
    10081 tgttgacgca gacatagctg cgcgtgatgc ccagcttggc cagcagcccc tgcagccgcc
    10141 gaccggcttc accgaccagg atgcgccgca cgatcgtctc gtgctgcgcc ggatcctgtc
    10201 cgatgaccag cacgcgcgcc gagccgtcga gccgcccgcg atggaacacc ggcccccatt
    10261 cgacgcggaa ctgatcgtcg ggatagacgt cggcttcggg atagtcctcg cacaggccca
    10321 tgaacggctc ggcgatgtag cccggatcga aggcatgtgt catgggcatc tccgcggag
//
LOCUS       DATCRO010000414         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_285667, whole genome shotgun sequence.
ACCESSION   DATCRO010000414 DATCRO010000000
VERSION     DATCRO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285667"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIN75_11220"
     CDS             <1..702
                     /locus_tag="VIN75_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY8974780.1"
                     /translation="FMGAERRSALMDPEELKNTAYHEAGHALVGWLMPKADPVHKVTI
                     VPRGRALGVTFFLPEKDNHCHDKIWYLSQISVAFGGRIAEEVFMNQATTGALSDFNSA
                     TQMARDMVMKYGMSDAMGPMVYAENEGEVFLGRSVTRTTNISEDTMRKVDAEVRRIID
                     EQYAVARNLIETHQDKMHAMANALLEYETIDNEQLRDIMEGRPPRPPKDWTPAPPKSG
                     GSGGAPVVDRTPATA"
     gene            776..1660
                     /gene="folP"
                     /locus_tag="VIN75_11225"
     CDS             776..1660
                     /gene="folP"
                     /locus_tag="VIN75_11225"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8974781.1"
                     /translation="MTNASPAPVPSAPTDARPLAWQTRRFRLELARPLVMGIVNVTPD
                     SFSDGGRFAQADAARAHCDLLVAQGADILDIGGESTRPGARLPDSTEQLARVRPVLEH
                     AVTLGVPVSVDTADPVVMREALAIGVDILNDVRALQAPGALAVAAGDPAVGICLMHMQ
                     GEPGTMQADPRYDDDDVVGAVATFLAARRDAAQAAGIARERIALDPGIGFGKTPAHNV
                     ALAVRQRRLLSLGCPLLLGWSRKSTLGAITGRPVGERLAASVAAALAAVQQGAAIVRV
                     HDVAETVDALKVWRTLAS"
     gene            1734..>2108
                     /gene="glmM"
                     /locus_tag="VIN75_11230"
     CDS             1734..>2108
                     /gene="glmM"
                     /locus_tag="VIN75_11230"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464306.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8974782.1"
                     /translation="MSRTYFGTDGIRGTVGTAPITADFMLRLGHAVGRVLRAKSPKAT
                     VLIGKDTRVSGYMIESALEAGFASAGVDVLLTGPLPTPGVAYLTRALRVDLGVVISAS
                     HNPFGDNGVKFFSASGEKLPDQW"
BASE COUNT          318 a          760 c          725 g          305 t
ORIGIN      
        1 ttcatggggg cggagcgccg ctcggccctg atggatcccg aggaactcaa gaacaccgcc
       61 taccacgagg ccggccatgc gctggtcggc tggctgatgc ccaaggccga tccggtgcac
      121 aaggtcacca tcgttccgcg cggccgcgcg ctgggcgtca cgttcttcct gccggagaag
      181 gacaaccatt gccacgacaa gatctggtac ctgagccaga tctcggtcgc cttcggtggc
      241 cgcatcgccg aggaagtgtt catgaaccag gccaccaccg gcgcgctgag cgacttcaac
      301 tcggccacgc agatggcgcg cgacatggtg atgaagtacg gcatgtccga cgcgatgggc
      361 ccgatggtct acgcggagaa cgagggcgag gtcttcctcg gccgctcggt cacgcgcacc
      421 accaacatca gcgaggacac gatgcgcaag gtggacgccg aggtccgccg catcatcgac
      481 gagcagtacg cggtcgcccg caacctgatc gagacgcacc aggacaagat gcacgcgatg
      541 gccaacgcgc tgctcgaata cgagacgatc gacaacgagc agctgcgcga catcatggag
      601 ggccgcccgc cgcgtcctcc gaaggactgg acgccggcgc cgccgaagtc cggcggctcc
      661 ggcggcgcgc cggtcgtcga tcgcacgcct gcgacggcct gatctcgaca agggccgcat
      721 gtgaacggca gcggggcttc ggccccgttc gccgtttttt cgtttgcgaa ccacgatgac
      781 caacgcttcg cccgccccgg tgccgtccgc gccgaccgat gcccggccgc tggcctggca
      841 gacgcgccgc ttccggctgg agctggcccg gccgctggtg atgggcatcg tcaacgtcac
      901 acccgattcg ttctcggacg gcggccgctt cgcgcaggcc gacgccgcgc gcgcccactg
      961 cgacctgctc gtggcgcagg gcgcggacat cctggacatc ggcggcgagt ccacccggcc
     1021 cggcgcccgg ctgcccgact ccaccgagca gctcgcgcgc gtgcgccccg ttctcgagca
     1081 cgcggtcacg ctcggcgtgc cggtctcggt cgacaccgcc gatccggtgg tgatgcgcga
     1141 ggcgctggcg atcggggtcg acatcctcaa cgacgtgcgc gcgctgcagg cgcccggcgc
     1201 tctggccgtg gcggcgggcg atcctgccgt gggcatctgc ctgatgcaca tgcagggcga
     1261 gcccggcacg atgcaggcgg atccccgcta cgacgatgac gacgtcgtcg gtgccgtcgc
     1321 gacgttcctc gcggcgcgtc gcgacgcggc tcaggccgcc ggcatcgcgc gcgagcgcat
     1381 cgccctcgat cccgggatcg gcttcggcaa gacgccggcg cacaatgtcg cgctggccgt
     1441 gcggcaacgg cggctgctgt ccctcggctg cccgctgctg ctgggctggt cgcgcaagtc
     1501 gacgctgggc gcgatcaccg ggcggccggt gggcgagcgg ctcgcggcga gcgtggcggc
     1561 ggcgctggcc gccgtgcagc agggggcggc catcgtgcgc gtgcacgacg tcgccgagac
     1621 cgtggacgcg ctgaaagtgt ggcgcaccct ggcttcgtga aacttccaac aacaacgcgc
     1681 ggtttcccgc accatcgttc gggccacccg cgaaatccac cgagaattcc cgcatgagca
     1741 gaacgtactt cggcaccgac ggcatccgtg gcaccgtcgg caccgccccg atcaccgccg
     1801 acttcatgct gcgcctgggc catgccgtgg gccgcgtcct gcgcgcgaag tcgcccaagg
     1861 caaccgtgct gatcggcaag gacacgcggg tgtccggcta catgatcgag tcggcgctgg
     1921 aagccggctt cgcgtcggcc ggcgtggacg tgctgctcac cggcccgcta cccaccccgg
     1981 gcgtcgccta cctgacgcgc gcgctgcgcg tcgaccttgg ggtggtcatc agcgcctcgc
     2041 acaacccgtt cggcgacaac ggcgtcaagt tcttctcggc ttccggcgag aagctgcccg
     2101 atcagtgg
//
LOCUS       DATCRO010000415         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_38098, whole genome shotgun sequence.
ACCESSION   DATCRO010000415 DATCRO010000000
VERSION     DATCRO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38098"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1407
                     /locus_tag="VIN75_11235"
     CDS             121..1407
                     /locus_tag="VIN75_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974783.1"
                     /translation="MSCPLPQAAARGVVRPRSPRGRWRRRAGATLLATLLAAPIARAG
                     VAPIRIGVIAPLTGASADFGTSVRQGAELAVDEINAAGGVLGRPLALVVKDDRGDPDT
                     GLAAATSLVTQERVDATIGFCNTGVAMKALDVFESRHQLLIVPCAQGTAITRRAHGED
                     SMVFRVAPADALNAAFLVGEIVDRRRLSRVAILADTTGYGDGGVADIGAQLARRGLQP
                     AYVGRFAADAPSLADQLLAARAAGAQALVVYTVGPGEAVAVKGRAAMHWDVPYFAPWT
                     LSFRSVLDAAGPAPLEGTMMTQSIIQDSANEARTAFIAAFSRRHAHRPPGSLMAAAQT
                     YDSVNLLVRALFATHGDTAPAAVRQALEQPREPHRGVVTTYDHPFSARDHDAFSVNMI
                     WLGVWRGGEIHYFHAADAGLSGAVRHKQEPDGSHPD"
     gene            1529..1702
                     /locus_tag="VIN75_11240"
     CDS             1529..1702
                     /locus_tag="VIN75_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEY8974784.1"
                     /translation="MRLLLLLPFIGLLAVPLFNTETPEFAGFPFFYWYMFAWVPLTSL
                     LIWLVNRNEKPEE"
     gene            1707..>2143
                     /locus_tag="VIN75_11245"
     CDS             1707..>2143
                     /locus_tag="VIN75_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY8974785.1"
                     /translation="MNTEINWTALSVFIFFFALVTVIGFLASRWQRGGAHKGAHLDEW
                     GLGGRNFGAWITWFLVGGDFYTAYTVIAVPALVYAVGAYGFFALPYTILVYPIVFTIM
                     PKLWGEAKRLGHVTAADVVYGRYGSRPLETAVALTGIVATMPY"
BASE COUNT          294 a          788 c          734 g          327 t
ORIGIN      
        1 gacccttaag tgatgccgcg caccgaccac cgtgctgcaa cggctgtgcc gtggcgcccg
       61 gcacgacaga ctgcgtccag agcgaaggcg catcgacctt cgccgcgttg gagacaagcc
      121 atgtcctgtc cgctgcccca ggccgccgcg cgcggcgtcg ttcgccccag gtcgccccga
      181 gggcgatggc gcaggagggc cggggccacg ctgctggcga cgctgcttgc cgcgccgatc
      241 gcgcgcgccg gcgtcgctcc gatccgcatc ggcgtcatcg ccccgctcac cggcgcatcc
      301 gccgacttcg gcaccagcgt gcgccagggc gccgaacttg ccgtcgacga gatcaacgcc
      361 gccggcggcg tgctgggccg cccgctggcg ctcgtggtca aggacgaccg cggcgatccg
      421 gacaccggcc tggccgcggc cacgtcgctg gtgacgcaag agcgcgtcga cgccacgatc
      481 ggcttctgca acaccggggt cgcgatgaag gcgctggacg tgttcgagtc gcgccaccag
      541 ctgctgatcg tgccgtgcgc gcagggcacc gcgatcacgc ggcgcgcgca tggcgaggac
      601 agcatggtgt tccgcgtcgc gccggcggac gcgctgaacg cggcgttcct cgtcggcgag
      661 atcgtcgacc gccgacggct gtcgcgcgtc gcgatcctgg ccgacaccac cggctatggc
      721 gacggcggcg tcgccgacat cggcgcgcag ctggcccggc gcggcctgca gccggcctac
      781 gtcggccgct tcgccgcgga cgcgccgtcg ctcgccgacc agctcctcgc ggcgcgcgcg
      841 gccggcgccc aggcgctggt cgtctacacc gtcggtccgg gcgaggcggt ggccgtcaag
      901 gggcgcgcgg cgatgcactg ggacgtcccg tacttcgcgc catggacgct gtcgttccgc
      961 agcgtgctgg acgccgcggg gccggcgccg ctggagggca cgatgatgac gcaaagcatc
     1021 atccaggact ccgccaacga ggcgcgcacc gcattcatcg cggccttctc gcgtcgacac
     1081 gcacaccggc ccccgggctc gctgatggcc gcggcgcaga cctacgacag cgtgaacctg
     1141 ctggtgcgcg cgctgttcgc cacgcacggc gacaccgcgc ccgccgccgt ccggcaggcg
     1201 ctcgagcagc cgcgcgagcc gcaccgcggc gtcgtcacca cctacgacca cccgttcagc
     1261 gcgcgcgacc atgacgcctt cagtgtcaac atgatctggc tgggcgtgtg gcgcggcggc
     1321 gagatccact acttccatgc cgcggacgcg gggctctccg gcgcggttcg gcacaaacag
     1381 gagcccgacg gttcgcatcc tgattagggg ttgtcccgta cgtggtgccg ctcgcgccgc
     1441 aacgcgacat gacggatgcg gcgcgcgccc cgaatttcga cactggagcc agagcgggca
     1501 atgtccccgc ccaacctgga gacaagacat gcgcctgcta ttgctgctgc cgttcatcgg
     1561 cctgctggcc gtcccgttgt tcaacaccga gacgccggag ttcgccggat tcccgttctt
     1621 ctactggtac atgttcgcgt gggtgccgct cacctcgctg ctgatctggc tggtcaaccg
     1681 caacgagaag ccggaggagt gaggccatga acaccgagat caactggacc gcgctgtcgg
     1741 tcttcatctt cttcttcgcg ctggtgaccg tcatcggctt cctggcctcg cgctggcagc
     1801 gcggcggcgc gcacaagggt gcgcacctgg acgagtgggg cctgggcggc cgcaacttcg
     1861 gcgcctggat cacgtggttc ctcgtgggcg gcgacttcta caccgcctac acggtgatcg
     1921 cggtgccggc gctggtctac gcggtcggcg cctacggctt cttcgcgctg ccgtacacca
     1981 tcctggtcta tccgatcgtc ttcaccatca tgcccaagct gtggggcgag gccaagcgac
     2041 tggggcacgt gacggcggcc gacgtcgtct acggccgcta cggctcgcgc ccgctcgaga
     2101 cggccgtcgc gctcaccggc atcgtcgcga cgatgcccta cat
//
LOCUS       DATCRO010000416         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_353026, whole genome shotgun sequence.
ACCESSION   DATCRO010000416 DATCRO010000000
VERSION     DATCRO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353026"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VIN75_11250"
     CDS             complement(<1..486)
                     /locus_tag="VIN75_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY8974786.1"
                     /translation="MPAKPDPAVHQLAPGVLATRLDSAQAFPRHAHDTYGIGRIRRGA
                     QRSWSGAGWVEAGPGDLLMVNPGEVHDGVPLDAAGRRWHMLYLAPAIVEAAAAHVLPA
                     GSALPMWRPVARDAIVARAFEHWHARLAARAGALACDEAQAALLAALLLRQAQVAPVR
                     LA"
     gene            574..1173
                     /gene="lptA"
                     /locus_tag="VIN75_11255"
     CDS             574..1173
                     /gene="lptA"
                     /locus_tag="VIN75_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY8974787.1"
                     /translation="MHVIDRLGAGALLALCCLAARAEHADRTRDIQVQAADARADQRQ
                     QTAEFSGDVVVSQGTLEIRADRVQMRAGPGGERLGVALGKSGAPVRFRQRGDRPDEWN
                     EGQADRVEYDSVANEVRLIGAASLRNLDGARVTQTITGDTITYDTARDTLSSAGADRR
                     ALLPEAAAPARRVTVVFAPRVTARGTTPAPPPEGALTPR"
     gene            1301..1933
                     /locus_tag="VIN75_11260"
     CDS             1301..1933
                     /locus_tag="VIN75_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8974788.1"
                     /translation="MNSKIQACVATLLAAAAPLALAANVATGANVTTTGSGFGLSAGW
                     GVGTPADPSSLVDGAFVADGQQWNLGTVYWQGDSTDSADTITITLAHAATVTGLLVQG
                     DNNDLYAVSYEALDGTWHSLPDAFSPHGTAADPGANSVYWGMGVSSESFAAITAQAFR
                     INASGDQSYAVAEFQATGQFLPTVPEPTSGLLVLAGLGALGAMARRRAVH"
     gene            2058..>2494
                     /locus_tag="VIN75_11265"
     CDS             2058..>2494
                     /locus_tag="VIN75_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEY8974789.1"
                     /translation="MQFESLVSLLESGGARFRVIEHPAEGRSDLVAQIRGTAPGQGAK
                     AMLCRSKDGDPAYGLVVLPGDRKLDFKRVAQALGLRKATLASPDEAMAATGCAIGAIP
                     PFVDGPALRLVVDPALVERFAEIAFNAGRLDRSIVLDSADYVR"
BASE COUNT          342 a          957 c          884 g          311 t
ORIGIN      
        1 cgcgagccgc accggcgcga cctgcgcctg gcgcagcagc agggccgcga gcagcgcggc
       61 ctgggcctcg tcgcacgcga gcgcccccgc acgcgcggcc aggcgcgcgt gccagtgctc
      121 gaacgcacgc gcgacgatgg cgtcgcgcgc gaccgggcgc cacatcggca gcgcgctgcc
      181 ggcgggcagc acgtgcgccg cggccgcctc cacgatggcg ggggcgaggt agagcatgtg
      241 ccagcgccgc cccgcggcgt ccagcggcac gccatcgtgc acctcgccgg ggttgaccat
      301 cagcaggtcg cccgggccgg cctcgaccca gcccgcgccg ctccacgagc gctgcgcgcc
      361 gcgccggatg cggccgatgc cgtaggtgtc gtgcgcgtgg cgcgggaacg cctgcgcgct
      421 gtcgagccgc gtggcgagca cgccgggagc gagctggtgg acggcgggat ccggcttggc
      481 gggcatgccc cagtatcgtc gcgatccgtg agcccacgaa gaagcatggc ggcaacatcc
      541 ccgcgctacg atcgcgccgg gagaccgcca agcatgcacg tcatcgatcg cctgggcgcc
      601 ggcgccctgc tcgccctgtg ctgcctcgcc gcgcgcgcgg agcacgccga tcgcacccgg
      661 gacatccagg tccaggccgc cgacgcgcgc gccgaccagc gccagcagac ggccgagttc
      721 agcggcgacg tcgtcgtcag ccagggcacg ctcgagatcc gcgccgaccg cgtgcagatg
      781 agagccggtc ccggcggcga gcggctgggc gtcgcgttgg gcaagagcgg cgcgcccgtg
      841 cgctttcgcc agcgcggcga ccggcccgac gaatggaacg aggggcaggc cgatcgcgtc
      901 gagtacgaca gcgtggccaa cgaggtgcgc ctgatcggcg cggcgtcgct gcgcaacctc
      961 gacggcgcgc gcgtcacgca gacgatcacc ggcgacacca tcacctacga caccgcccgc
     1021 gacacgctct cgtcggccgg cgccgaccgg cgcgccctgc tgccggaagc ggcggcgccg
     1081 gcccggcgcg tgacggtcgt gttcgcgccg cgcgtgaccg cccgcggcac gacgcccgcg
     1141 ccgccgcctg aaggggcgct gacaccccgc tgaagcacct ccccaaagca ggtgacttgc
     1201 gcgccatccc ccctcgtctg cgggatgctt gcccatggag cgcgccgtta gcatcgcgca
     1261 ccagcgctca tcccggcgcg catcaacgag agggttcgcg atgaattcga agatccaggc
     1321 ctgcgtggcc acgctgctgg ccgccgccgc cccgctggcg ctcgctgcca atgtcgccac
     1381 cggcgccaac gtcaccacca ccgggtccgg tttcggcctg agcgcgggct ggggcgtggg
     1441 cacgccggcc gatccgtcca gcctcgtcga cggcgcgttc gtggccgacg gccagcaatg
     1501 gaacctgggc accgtgtact ggcagggcga cagcaccgac agcgccgaca cgatcaccat
     1561 cacgctggcc cacgcggcca ccgtcaccgg cctgctggtg caaggcgaca acaacgatct
     1621 gtacgccgtc agctacgaag ccctcgacgg cacctggcac tcgctgccgg acgccttctc
     1681 gccgcacggc acggcggccg accccggcgc gaactcggtg tactggggca tgggtgtctc
     1741 gtccgagagc ttcgcggcga tcaccgcgca ggcgttccgc atcaacgcga gcggcgacca
     1801 gtcctacgcc gtcgccgagt tccaggccac cggccagttc ctgcccacgg tgcccgagcc
     1861 gacctccggc ctgctggtgc tcgccggact cggcgcactc ggcgcgatgg cgcgccgccg
     1921 cgccgtgcac tgacgcctcg tcctgcggcg cggccggcat tcgccgcgag gcgggtcccg
     1981 gccgtttcca ttgtggcgcg ggcaggccgc cgcccttcga taatccgcga ttccgaatcc
     2041 tcgacgagag ccgccccatg cagttcgaat cgctcgtctc gttgctcgaa tccggcggcg
     2101 cgcgctttcg cgtcatcgag catccggccg aaggccgctc cgacctcgtc gcgcagattc
     2161 gcggcaccgc gcccggccag ggcgccaagg cgatgctgtg ccggagcaag gacggcgatc
     2221 cggcgtatgg gctcgtggtg ctgccgggcg atcgcaagct cgacttcaag cgcgtcgcgc
     2281 aggccctcgg actgaggaag gcgacgctcg cgagccccga cgaggcgatg gccgccaccg
     2341 gctgcgcgat cggcgccatc ccgccgttcg tcgacgggcc ggcactgcgc ctggtcgtcg
     2401 atccggcgct cgtcgagcgg ttcgcggaga tcgcattcaa cgccggccgg ctggatcgct
     2461 cgatcgtgct ggactcggcc gactacgtgc ggat
//
LOCUS       DATCRO010000417        11656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_150861, whole genome shotgun sequence.
ACCESSION   DATCRO010000417 DATCRO010000000
VERSION     DATCRO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150861"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VIN75_11270"
     CDS             complement(<1..944)
                     /locus_tag="VIN75_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974790.1"
                     /translation="MPKLPRIRWTLAACGLAIATTIVVVTACSSGPKPGEVLDEAMQA
                     GRSAESFAQADEDYFHDMDNGVALTPEEIRGRNMWIVWSGGNDRFWDGLTTTTFGAFD
                     LLKIVSSHPDLGFTRANRWDNLGLVNEPCFQAATGPDPKRFGLWLDQRRADCPADPFE
                     NETKYPGVKIGARGSAKMPDGSTFPVGSFYGYATGIAGLRLFPNPKFDEAAAARWDPR
                     RYYTDPTYYNDKDLVRPYRVGMSCGFCHVGPSPTHPPADPAAPQWANLSSSVGAQYMW
                     VDRLFIFNSNKPEGQRNFMYQLAHTFRPGSMDTSLIST"
     gene            1168..2028
                     /locus_tag="VIN75_11275"
     CDS             1168..2028
                     /locus_tag="VIN75_11275"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY8974791.1"
                     /translation="MPYDPSRDSLLHPWSRPPLFDAGNVGQKPQDGILAECARLAYFR
                     FERDDHQRAQWRAALTLLGAQDVAWFSEPATDTQAYAFHRPATNQSIVSFRGTEPDSV
                     SDLGSDIDTLMIDWVAQPGRPGIRVHAGFAGALDRVAAMPAFADWITGHQGALVFTGH
                     SLGAALATLGAARWGANRLVTFGSPRVGDASLTNRVTAQAADIRRYVDCCDLVTELPP
                     PIGDFEHVGEMTYIDSDGGLPPGNGIPVAVDRAQARVDYLEQLAWRFGTVPVRDLADH
                     APINYVRAWF"
     gene            complement(2025..3026)
                     /locus_tag="VIN75_11280"
     CDS             complement(2025..3026)
                     /locus_tag="VIN75_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8974792.1"
                     /translation="MAEPAPLLSVRDLQVHFKVRDRKAWPWTPAAALKAVDGVSFELR
                     AGETLGIVGESGCGKSTLARACLNLIPATGGEVAWQGKVLRPDDHAAWLAARRDIQII
                     FQDPLASLNPRMTVARIIGEPLRIHRPELGDAQIHEKVLAVMAEVGLTPQQVNRYPHE
                     FSGGQCQRIGIARALILEPKLIICDEPVSALDVSIRAQIVNLLQELQRRKGLALIFIA
                     HDLAVVKHISDRVLVLYLGKAMEIAQGDALYARPGHPYTEALLSAVPVPDPRIERGKV
                     LQLLSGDMPSPLAPPSGCVFRTRCPKAVPRCAQELPALRALDGLTSAACLLAPGRAG"
     gene            complement(3019..4014)
                     /locus_tag="VIN75_11285"
     CDS             complement(3019..4014)
                     /locus_tag="VIN75_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8974793.1"
                     /translation="MPVLEVRDLSVRFTTNDGPVYAVNDVAFRLGRGQTLGIVGESGS
                     GKSQTMLAMMGLLARNGTATGQVLHHGRNLVGLPAAELNAVRGDRIAMIFQDPMTSLN
                     PYLTVERQMTEVLELHRGLGRREARVRAIQALDDVKIPDAKRRIDFYPHEFSGGMRQR
                     VMIAMALLCEPEVLIADEPTTALDATVQAQIVQLLRELQREHGTAIIMITHDLGVVAG
                     LCDDVLVMYGGRVMEHASADDIFHRPSHPYTRGLLAALPRLDGGAETLRSIPGNPPNM
                     NALPPGCPFSARCRFVQDRCATTLPLLQPLGPGAPTLRACHRAVAELDAMEAADG"
     gene            complement(4098..4982)
                     /locus_tag="VIN75_11290"
     CDS             complement(4098..4982)
                     /locus_tag="VIN75_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8974794.1"
                     /translation="MSATLPAVDAAPAGRSPWRDARVRFMRHRAAVASVVVLALVALA
                     CVFGPMLLPNAFDSVDWNLMSAPPTRAGWHLFGTDELGRDLLVRTLIGGRISLLVGLL
                     ATLVSATIGVVWGAVAGFAGGRVDSVMMRIVDMAYAVPYLLIAILVVTVFGRDILLVV
                     AAIAAFSWLDMARVVRGQTLSIKSREFVEAARAIGVPTWRIIFQHVVPNLMGVVVIYT
                     SIVVPGVILSESVLSFLGLGVQEPMTSWGVLIHDGAAVMGATPWMILFPAAALSITLY
                     CANFIGDGLRDALDPKDR"
     gene            complement(4984..5904)
                     /gene="oppB"
                     /locus_tag="VIN75_11295"
     CDS             complement(4984..5904)
                     /gene="oppB"
                     /locus_tag="VIN75_11295"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HEY8974795.1"
                     /translation="MWAYVVRRLLVTVPTVLAVVTLCYVLLHLTPGGPFDAERKVSDA
                     VLANLQAKYHLDLPPWKQYLYYLRDLLHGDLGASFRYADWSVNDLVARALPVSLAIGG
                     TAMVVAAVLGVALGITAALRQNSVVDVGVMLVANIGSVLPSFVLGPVLVLVFAILLPW
                     LPSGGWDDFAPRYMILPIALLTLINVATIGRIMRGSLIEVLGSNFIRTARAKGLPMRV
                     VVMRHALRPALLPVVSVIGPLAISSITSAIVTESWFSIPGLGRLIVNGASDRDYTLVL
                     GLVVLVTVLAVLLNLLVDLAYAWLDPKIRY"
     gene            complement(5923..7548)
                     /locus_tag="VIN75_11300"
     CDS             complement(5923..7548)
                     /locus_tag="VIN75_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8974796.1"
                     /translation="MNTRHKPSLARALATLALAAVSCVAVQAAVIPPGTVLAARQEMV
                     RNNGAEPESLDPAYIESVPAGEIDRDLFEGLTSSDHHSNVVPGVAESWKQVDPTTWVF
                     KLRKNAVWSNGTPVTAEDFVYSWRRFLDPKTAAKIATTYGIYLANGLDVVNGKKPVTD
                     LGVRAIDPQTLEVKTAGPVPFLPALVAVAQYGPQPRAIVEKYGKDWTKPGNMVSNGAY
                     VLKDWQVNSKVVVEKNPKYWDAANVQLTRVTFLCVEDGNADLKLYQSGEEDYMLQLSP
                     GAYNALKAQYPAEMHNGPLLGLRFYALNTSDPLLKDVRVRKALSMVIDRQILSDKVTA
                     DGQAPLYGLAVKGLGAIQQTRYDWADWPMDRRVAEARKLLAEAGVKPGTHLKFTYNNS
                     DYHKKMAIFAASEWKTKLGLDTDMDSLEFKVLIRKRHDGDFQIARHGWVATFNDATLF
                     LALVQCGSEVNDQRSCNRKADELIAQGNASTDPARREALLTQAARLAMDDYPLIPLLQ
                     YSIPRLIKPWIGGYDDANGMDRFRSKDFYIIKH"
     gene            complement(7641..9257)
                     /locus_tag="VIN75_11305"
     CDS             complement(7641..9257)
                     /locus_tag="VIN75_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8974797.1"
                     /translation="MTSKLRRCAVHLAAAAVAASLPAAAAVVPPGTVIAAKQELVRNN
                     GAEPESLDPQLIESTNAFNILIDLFEGLTALDNHARVVPGVAESWKQVEPTTWVFRLR
                     KDARWSNGEPVTAPDFVYGWHRLVDPKNAAPLGSTMGAYVLNGVAITQGKMPVSALGV
                     RALDAGTLEVRTPGPLPFLPYLLANVQFSPVPHALVEKYGRDWTRPGNLVGNGAFVLK
                     EWQVNSKVVVEKSPTYWDASHVVLTRSTYLPVEDNNADLKLYQSGEDDMTNRTPPGAY
                     EALKAQYPREMHNGTQLSIRFWALNNTDPLLRDVRVRKALSMVIDRELMAAKVTADGQ
                     LPIYGLVLKGSEGVDPVRYEWADWPMDRRVAEARRLLAEAGVKPGTRLKYAYNTSDYH
                     KKLALFTASEWKTKLGLETELEAMEYKVLIHRRESAQYQVARHAWTPGYADITGLLAL
                     VECGNDANDNKSCSRAGDDLMHQAAALSDPVRRKALLTQAVRVEMEDYPMIPLLQMSL
                     PRLVKPWVGGYDDANDQDAFRSKDLYILRH"
     gene            9481..10674
                     /locus_tag="VIN75_11310"
     CDS             9481..10674
                     /locus_tag="VIN75_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8974798.1"
                     /translation="MRQPLRVAASALLLAVLAACQTTPTAPGPGNVSRLPPGNAERDG
                     PEAHPPANLEAVPDAEPRIEAIRNGGPNKPYEIGGKTYTPLTDDRALTEKGLASWYGK
                     KFHGRRTASGEAYNMYAMTAAHKTLPIPSYARVRNPANGKEVIVRVNDRGPFSPGRVI
                     DLSYTAALKLGVLGGVTPVEVTRITADDIRTGAAFRKAAPGEAPTAVTLGDLDRSVPT
                     RPGVTRPRPTAVAAPAVATALPPAAGDVIATAADTARPGMDTGTQAAPQSPATTPGVD
                     LGAAAAVDATTPAPGTVTTVSESVVSGPSPDKPDERARADTTAGIGWWLQLGAFRQRD
                     GALDFQRKLIDEQPWLAPLLAVFSDRGLNKLQAGPYTSRDEARSAADKVRTALRLVPT
                     IVEKR"
     gene            complement(10754..11026)
                     /locus_tag="VIN75_11315"
     CDS             complement(10754..11026)
                     /locus_tag="VIN75_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974799.1"
                     /translation="MTAITSGTYGHQPIFTGAIRQLVAGLFSSSRPARKPAAATVRPA
                     LVANAHRVRIREANDVRALARSVEMHSPGFAADLYAAASRHEGYDD"
     gene            11127..>11656
                     /locus_tag="VIN75_11320"
     CDS             11127..>11656
                     /locus_tag="VIN75_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974800.1"
                     /translation="MAFISCSTVNLVNFRTLDLNLLRVFDAVMAEGSLTRAAERLAMT
                     QPAASHALKRLHVAVGEELLHRTAHGMRPTVRAEVLWPQVRSALAQLQGALSPPDFDP
                     ARDAATFSIAMADATAAMLLPALVVAIENAEAIANVRILPLTTRDPRRLVEDGDADLA
                     VGYFPNAIAAVIAQGQ"
BASE COUNT         1740 a         4090 c         4089 g         1737 t
ORIGIN      
        1 tcggtggaga tcagcgacgt gtccatgctg cccgggcgga acgtgtgcgc cagctggtac
       61 atgaagttgc gctggccctc gggcttgttc gaattgaaga tgaacagccg gtcgacccac
      121 atgtactgcg cgcccaccga cgaactgagg ttggcccatt gcggcgccgc cggatcggcc
      181 ggcggatggg tcgggctcgg gccgacatgg cagaagccgc aggacatgcc cacgcggtat
      241 ggccgcacga ggtccttgtc gttgtagtac gtgggatcgg tgtagtagcg cctgggatcc
      301 cagcgcgcag cggcggcctc gtcgaacttc gggttcggga acaggcgcag gccggcgatg
      361 cccgtggcgt agccgtagaa cgagccgaca gggaaggtac tgccgtcggg catcttcgcg
      421 ctgccgcgcg cgccgatctt cacgccggga tacttcgtct cgttctcgaa cgggtcggcc
      481 gggcagtcgg cgcggcgctg gtcgagccac aggccgaagc gcttcgggtc ggggccggtg
      541 gcggcctgga agcagggctc gttgaccagg ccgaggttgt cccagcggtt ggcgcgcgtg
      601 aagccgagat cgggatggga ggagacgatc ttgagcaggt cgaacgcgcc gaacgtggtg
      661 gtggtcaggc cgtcccagaa gcggtcgttg ccgccgctcc agacgatcca catgttgcgg
      721 cccctgatct cctcgggcgt cagcgcgacg ccgttgtcca tgtcgtggaa gtagtcctcg
      781 tcggcctgcg cgaacgattc cgcgctgcga ccggcctgca tcgcctcgtc caggacttct
      841 cccggcttgg ggccggagct gcaggccgtg acgaccacga tcgtcgtcgc gatcgccagc
      901 ccacaggcgg ccagggtcca acggatgcgc ggaagcttgg gcatgctgtc tctctccaga
      961 cctgaaacct cgggcggcgc gtgccctccc ggcccgcgcg gacatggaaa tcgactatag
     1021 gcgcgaagcc ggcgccggtt aagggctggt gaacgtcatc cccctacgtt gccagggcgc
     1081 cccggcgccg gcgattgcag cgccccgcga gaacgcacag actgccggct gtccgatcaa
     1141 gtcgcttgtc aggagtccac cgtggccatg ccgtacgacc cttcgcgcga ttcgctgctg
     1201 cacccgtggt cgaggccgcc cttgttcgat gcgggcaacg tcggccagaa gccgcaggac
     1261 gggatcctgg ccgagtgcgc ccgcctggcc tacttccggt tcgagcgcga cgaccaccag
     1321 cgcgcgcagt ggcgcgccgc gctcaccctc ctgggcgcgc aggacgtcgc ctggttctcc
     1381 gaaccggcga ccgatacgca ggcctatgcc ttccaccgtc cggcgaccaa ccagagcatc
     1441 gtgtccttcc gcggtaccga gccggactcc gtgtccgacc tgggcagcga catcgacacc
     1501 ctgatgatcg actgggtcgc gcagccgggc cggcccggca tccgcgtcca tgcgggcttc
     1561 gcgggcgcgc tcgaccgcgt cgccgccatg ccggcgttcg ccgactggat cacgggccat
     1621 caaggcgccc tcgtcttcac cggccacagc ctcggcgccg cgctggccac gctcggcgcc
     1681 gcgcgctggg gagcgaaccg cctcgtcacc ttcggatcgc cgcgcgtcgg cgatgcgtca
     1741 ctcacgaacc gggtgaccgc gcaggcggcc gacatccgcc gctatgtcga ctgctgcgac
     1801 ctcgtcacgg agctgcctcc gccgatcggc gacttcgagc acgtcggcga gatgacctac
     1861 atcgacagcg acggcgggct gcctcccggc aacggaatcc cggtcgccgt cgaccgcgcg
     1921 caagcccgcg tcgactacct cgagcagctc gcctggcgct tcgggacggt gccggtgcgg
     1981 gatctggccg atcacgcgcc gatcaactac gtgcgcgcct ggttctagcc cgctcgcccg
     2041 ggcgccagca ggcacgccgc cgacgtcagc ccatcgagcg cgcgcagcgc cggcagttcc
     2101 tgcgcgcagc gcggcaccgc cttcgggcag cgcgtgcgga acacgcagcc cgagggcggc
     2161 gcgagcgggg acggcatgtc gcccgagagc agctgcagca ccttgccgcg ctcgatgcgc
     2221 gggtcgggga cgggcaccgc cgacagcagg gcctccgtgt acggatgccc cggccgcgcg
     2281 tacagggcgt cgccctgcgc gatctccatg gccttgccca ggtacagcac cagcacgcgg
     2341 tcgctgatgt gcttgaccac cgcgaggtcg tgggcgatga agatcagcgc caggcccttg
     2401 cggcgctgca gctcctgcag caggttgacg atctgcgcac ggatcgacac gtcgagcgcc
     2461 gacaccggct cgtcgcagat gatcagcttc ggttcgagga tcagcgcccg cgcgatgccg
     2521 atgcgctggc actggccgcc ggaaaactcg tgcgggtagc ggttgacctg ctgcggcgtc
     2581 aggcccacct cggccatcac cgcgagcacc ttctcgtgga tctgggcgtc gcccagctcc
     2641 ggccggtgga tgcgcagcgg ctcgccgatg atgcgcgcca ccgtcatgcg cgggttcagg
     2701 gacgcgagcg gatcctggaa gatgatctgg atgtcgcgcc gcgccgccag ccacgcggcg
     2761 tggtcgtcgg gccgcagcac cttgccttgc caggcgacct cgccgccggt ggccgggatc
     2821 aggttcaggc aggcgcgcgc cagggtcgac ttgccgcagc ccgactcgcc gacgatgccg
     2881 agcgtctcgc cggcgcgcag ctcgaacgag acgccgtcga cggccttgag cgcggcggcg
     2941 ggcgtccacg gccaggcctt gcggtcgcgc accttgaagt gcacctgcag gtcgcgcacg
     3001 gacagcagcg gcgccggctc agccatcggc cgcctccatc gcgtcgagct cggccaccgc
     3061 gcggtggcac gcgcgcagcg tgggcgcccc cggccccagc ggctgcagca gcggcagcgt
     3121 cgtggcgcag cgatcctgca cgaagcggca gcgcgcggag aacgggcagc cgggcggcag
     3181 cgcgttcatg ttgggcgggt tgccggggat gctgcgcagc gtctcggcgc cgccgtccag
     3241 gcgcggcagc gcggcgagca ggccgcgcgt gtacgggtgc gacgggcggt ggaagatgtc
     3301 gtcggccgag gcgtgttcca tcacgcggcc gccgtacatc acgagcacgt cgtcgcacag
     3361 cccggccacc acgcccagat cgtgcgtgat catgatgatg gccgtgccgt gctcgcgctg
     3421 cagctcgcgc agcagttgca cgatctgcgc ctgcacggtg gcgtcgagcg cggtggtggg
     3481 ctcgtcggcg atcagcacct cgggctcgca cagcagcgcc atggcgatca tcacgcgctg
     3541 gcgcatgccg ccggagaact cgtgcgggta gaagtcgatg cgtcgcttcg cgtcggggat
     3601 cttgacgtcg tccagcgcct ggatcgcccg cacgcgcgcc tcgcgccgcc ccaggccgcg
     3661 gtgcagttcc agcacctcgg tcatctggcg ctcgaccgtc aggtacgggt tgagcgaggt
     3721 catcggatcc tggaagatca tcgcgatgcg gtcgccgcgc accgcgttca gttccgcggc
     3781 gggcagcccc accaggttgc ggccgtgatg cagcacctgg cccgtggccg tgccgttgcg
     3841 cgcgagcagg cccatcatcg ccagcatcgt ctgcgacttg cccgagcccg attcgccgac
     3901 gatgcccagg gtctggccgc gtccgagccg gaaggcgacg tcgttgaccg cgtagaccgg
     3961 gccgtcgttg gtggtgaagc gcacgctgag gtcgcgcacc tcgagcaccg gcacgttcgt
     4021 cgcgtcgatc acagcgaagc ctccgccctg cgggctgcgc agcgaacgcc ttcgggcggc
     4081 cgggcgtcgt tcatggctca gcggtccttc ggatcgagcg cgtcacgcag cccgtcgccg
     4141 atgaagttgg cgcagtacag cgtgatcgac agcgccgcgg ccggaaacag gatcatccac
     4201 ggcgtggcgc ccatcacggc ggcgccgtcg tggatcagca cgccccagct ggtcatcggc
     4261 tcctgcacgc ccagccccag gaacgacagc accgattcgc tcaggatcac gcccggcacc
     4321 acgatcgacg tgtagatgac gaccacgccc atcaggttgg gcacgacgtg ctggaagatg
     4381 atgcgccacg tgggcacccc gatcgcacgc gcggcctcga cgaactcgcg cgacttgatc
     4441 gacagcgtct ggccgcgcac gacgcgcgcc atgtccagcc acgagaacgc ggcaatggcg
     4501 gccaccacca gcaggatgtc gcgcccgaac accgtgacca cgaggatggc gatgagcagg
     4561 tagggcacgg cgtaggccat gtcgacgatg cgcatcatca cgctgtccac gcgcccgccg
     4621 gcaaagccgg ccacggcgcc ccacacgacg ccgatggtgg ccgacaccag cgtcgccagc
     4681 agccccacca gcagcgagat gcgcccgccg atcagcgtgc gcaccagcag gtcgcggccc
     4741 agttcgtcgg tgccgaacag gtgccagccg gcgcgcgtgg gcggcgcgct catcaggttc
     4801 cagtcgacgg agtcgaacgc gttgggcagc agcatcggac cgaacacgca ggccagcgcg
     4861 accagcgcga gcaccacgac gctcgcgacc gccgcgcggt ggcgcatgaa gcgcacgcgg
     4921 gcgtcgcgcc aggggctgcg gcccgcgggc gcggcgtcga cggcgggcaa ggtcgcgctc
     4981 atctcagtaa cggatcttcg gatcgagcca cgcgtacgcg aggtcgacca gcaggttgag
     5041 cagcacggcc agcaccgtga ccagcaccac gaggcccagc accagcgtgt agtcgcggtc
     5101 ggacgcaccg ttgacgatca gcctgcccag ccccgggatc gagaaccagg actcggtgac
     5161 gatcgccgac gtgatcgacg agatcgccag cgggccgatg acggacacca ccggcagcag
     5221 cgccgggcgc agcgcgtggc gcatgaccac cacgcgcatc ggcaacccct tcgcgcgcgc
     5281 ggtgcggatg aagttcgagc cgagcacctc gatcaggctg ccgcgcatga tgcggccgat
     5341 ggtggccacg ttgatcagcg tgagcagggc gatcggcagg atcatgtagc gcggcgcgaa
     5401 gtcgtcccac ccgcccgacg gcagccaggg cagcaggatc gcgaacacga gcaccaggac
     5461 cggccccagc acgaacgacg gcagcacgct gccgatgttg gccaccagca tgacgccgac
     5521 gtcgaccacg ctgttctggc gcagcgccgc ggtgatgccc agcgcgacgc ccagcaccgc
     5581 ggccaccacc atcgccgtgc ccccgatggc cagcgacacg ggcagcgccc gcgcgacgag
     5641 gtcgttgacc gaccagtcgg cgtagcggaa cgacgcgccg aggtcgccgt gcagcaggtc
     5701 ccgcaggtag tacaggtact gcttccacgg cggcaggtcc aggtggtact tggcctgcag
     5761 gttggccagc accgcgtcgg acaccttgcg ctccgcgtcg aacggcccgc ccggcgtcag
     5821 gtgcagcagc acgtagcaca gcgtgaccac ggcgagcacc gtggggacgg taaccagcag
     5881 gcggcggacg acgtaggccc acatgacgtc aacgcttcgc ggtcagtgct tgatgatgta
     5941 gaagtccttg ctgcggaacc ggtccatgcc gttcgcgtcg tcgtagccgc cgatccaggg
     6001 cttgatcagg cgcgggatcg agtactgcag cagcgggatg agcgggtagt cgtccatcgc
     6061 gagcctggcg gcctgcgtca ggagggcttc gcgcctggcc gggtcggtgc tggcgttgcc
     6121 ctgcgcgatc agctcgtcgg ccttcctgtt gcagctgcgc tggtcgttga cctccgagcc
     6181 gcactgcacc agcgcgagga acagcgtggc gtcgttgaac gtggccaccc agccgtggcg
     6241 cgcgatctgg aagtcgccgt cgtggcgctt gcggatcagc accttgaact cgaggctgtc
     6301 catgtcggtg tccagcccca gcttggtctt ccactcggag gcggcgaaga tcgccatctt
     6361 cttgtggtag tcgctgttgt tgtaggtgaa cttcaggtgc gtgccgggct tgacgcccgc
     6421 ttccgcgagc aacttcctcg cctcggcgac gcgcctgtcc atcggccagt cggcccagtc
     6481 gtagcgcgtc tgctggatgg cgcccaggcc cttgaccgcc aggccgtaca gcggcgcctg
     6541 gccgtcggcg gtcaccttgt cggacaggat ctggcggtcg atcaccatcg acagcgcctt
     6601 gcggacgcgc acgtccttga gcagagggtc gctggtgttg agcgcgtaga agcgcaggcc
     6661 cagcaacggg ccgttgtgca tctcggccgg gtattgcgcc ttcagtgcgt tgtaggcgcc
     6721 cggcgagagc tggagcatgt agtcctcctc gcccgactgg tacagcttca ggtcggcgtt
     6781 gccgtcctcc acgcacagga aggtgacgcg cgtgagctgc acgttggccg cgtcccagta
     6841 cttcggattc ttctcgacga cgaccttgct gttgacctgc cagtccttga gcacgtacgc
     6901 gccgttgctc accatgttgc ccggcttggt ccagtccttg ccgtacttct cgacgatcgc
     6961 cctgggctgc ggcccgtact gcgccacggc caccagcgcc ggcaggaacg gcaccgggcc
     7021 ggccgtcttg acctccagcg tctgcgggtc gatcgcgcgc acgcccaggt cggtgacggg
     7081 cttcttgccg ttgacgacgt ccaggccgtt ggccaggtag atgccgtagg tggtggcgat
     7141 cttggccgcg gtcttcgggt cgaggaagcg gcgccacgag tagacgaagt cctcggccgt
     7201 cacgggcgtg ccgttggacc acaccgcgtt cttgcgcagc ttgaacaccc aggtcgtcgg
     7261 gtcgacctgc ttccacgact cggccacgcc gggcacgacg ttgctgtggt ggtcggagga
     7321 ggtcagccct tcgaacaggt cgcgatcgat ctcgccggcg ggcaccgatt cgatgtacgc
     7381 gggatcgagc gactcgggct ccgcgccgtt gttgcgcacc atctcctgcc tggctgccag
     7441 cacggtgcca ggcgggatga cggccgcctg gacggccacg cacgagacgg cggctagcgc
     7501 cagggtggcg agggctcgag cgagcgacgg cttgtggcgc gtgttcattt ccaggggact
     7561 ccgtgcgtga cttggggtga agtcagcgga tcagtatagg ggccggccac gcgcgccacc
     7621 ctcggcgatt gcccgcggga tcagtgcctg aggatgtaga gatccttgct gcggaacgcg
     7681 tcctggtcgt tcgcgtcgtc atagcctccg acccagggct tgaccaggcg cggcagcgac
     7741 atctgcagca gcgggatcat cggatagtct tccatctcga cgcgcaccgc ctgcgtcagc
     7801 agcgcctttc gcctgacggg gtcggacagc gccgcggcct ggtgcatcag gtcgtcgccc
     7861 gcgcggctgc agctcttgtt gtcgttggcg tcgttgccgc attccaccaa cgccagcagg
     7921 ccggtgatgt cggcgtagcc gggcgtccag gcgtggcgcg cgacctggta ctgcgcgctc
     7981 tcgcggcggt ggatgagcac cttgtattcc atcgcctcga gctccgtctc caggcccagc
     8041 ttggtcttcc attccgaggc ggtgaacagc gccagcttct tgtgatagtc gctcgtgttg
     8101 taggcgtact tcaggcgggt gccgggcttg acgccggcct cggccagcag cctgcgcgcc
     8161 tcggccacgc gcctgtccat gggccagtcg gcccattcgt agcgcaccgg gtcgacgccc
     8221 tcgctgccct tgagcacgag cccgtagatc ggcaattggc cgtcggcggt caccttggcg
     8281 gccatgagct cgcggtcgat caccatcgac agcgccttgc gcacgcgcac gtcgcgcagc
     8341 aacgggtcgg tgttgttgag tgcccagaag cggatcgaga gctgcgtgcc gttgtgcatc
     8401 tccctggggt attgcgcctt gagcgcctcg tatgcgccgg gcggcgtgcg gttggtcatg
     8461 tcgtcttcgc cggactggta cagcttcagg tcggcgttgt tgtcctccac cggcaggtag
     8521 gtcgagcgcg tgagcacgac atggctggcg tcccagtagg tcggactctt ctcgaccacc
     8581 accttgctgt tgacctgcca ctccttgagc acgaatgcgc cgttgcccac caggttgccg
     8641 ggccgggtcc agtcgcgccc gtacttctcg acgagcgcat gcggcaccgg gctgaactgc
     8701 acgttggcca gcaggtaggg caggaacggc agcgggccgg gcgtcctgac ctccagcgtg
     8761 ccggcatcga gggcccgcac gcccaacgcg gacaccggca tcttgccctg cgtgatggcc
     8821 acgccgttca acacgtaggc acccatcgtc gaaccgagcg gcgccgcgtt cttcgggtcg
     8881 accaggcgat gccagccgta cacgaagtcc ggcgccgtca ccggctcgcc gttggaccag
     8941 cgcgcgtcct tgcgcagcct gaacacccag gtcgtcggtt cgacctgctt ccacgattcc
     9001 gcgacgccgg gcaccaccct ggcgtggttg tcgagcgcgg tcaggccctc gaacaggtcg
     9061 atcaggatgt tgaaggcgtt ggtcgactcg atcagctgcg gatccagcga ctcgggctcg
     9121 gcgccgttgt tgcgcaccag ttcctgcttc gcggcgatca cggtgccggg cgggacgacg
     9181 gccgccgcgg cgggcaggct ggcggcgacg gccgcggccg ccaggtgcac cgcgcagcgg
     9241 cgaagcttcg atgtcatggg caaacgtcct cgcatcggcg tgcggcaggc gcgcgcccgt
     9301 gggtgtgcaa ggattgaaac acatcgtccg ggcggtgagc gtcgatgccg gcgcgggacg
     9361 gcctcggtgt aagcttccct tgcgccggcc gacgcgccgc gcgcgacgcc cgcccacggg
     9421 ccacagactc gccgaatgcc cgatcgtgaa ccgtcctccc cgccgctaac cgggacctgc
     9481 gtgcgacagc cgctgcgcgt cgccgcgtcg gcgctgctgc tcgcggtgct ggccgcctgc
     9541 cagacgacgc ccacggcgcc cggccccggc aacgtctcgc gcctgccgcc cggcaacgcc
     9601 gagcgcgacg gccccgaggc gcatccgccg gccaacctcg aggccgtgcc cgacgccgag
     9661 cccaggatcg aggccatccg caacggcggg cccaacaagc cctacgagat cggcggcaag
     9721 acgtacacgc cgctgaccga cgatcgcgcg ctcaccgaga agggcctggc ctcgtggtac
     9781 ggcaagaagt tccacggccg gcgcaccgcc agcggcgagg cctacaacat gtacgcgatg
     9841 acggcggcgc acaagacgct gccgatcccg agctacgcac gcgtgcgcaa cccggccaac
     9901 ggcaaggaag tgatcgtgcg cgtgaacgac cgcggcccgt tctcgccggg tcgcgtgatc
     9961 gacctgtcct acaccgccgc gctgaagctg ggcgtgctgg gcggcgtgac gccggtggag
    10021 gtgacgcgca tcaccgcgga cgacatccgc accggcgccg cgttccgcaa ggccgcgccg
    10081 ggggaggcgc cgacggcggt gacgctgggc gacctcgaca ggagcgtgcc cacgcgcccg
    10141 ggcgtgacgc gaccgcgccc gaccgccgtg gcggcgcccg ccgtcgcgac cgcgctgccg
    10201 ccggcagccg gcgacgtcat cgcgacggcc gcggacacgg cccggccagg gatggacacg
    10261 ggcacgcagg ccgcgccgca atcgcccgcg acaacgcccg gcgtcgacct cggcgcggcg
    10321 gcggcggtgg acgcgaccac gcccgcgcca ggcacggtga cgacggtcag cgaatcggtg
    10381 gtctcgggcc cgtcgccgga caagcccgat gaaagggcgc gcgccgacac caccgccggc
    10441 atcggctggt ggctgcagtt gggcgccttc aggcagcgcg acggcgcgct cgacttccag
    10501 cgcaagctca tcgacgagca accctggctg gccccgctgc tggcggtctt cagcgaccgc
    10561 ggtctcaaca agctgcaggc ggggccctac acgtcgcggg acgaggccag gagcgcggcg
    10621 gacaaggtcc gcacggcgct tcgactggtg cccacgatcg ttgaaaagcg ctaggggaac
    10681 gcggcgccgg ctccctggcg gcgcgaagcg ccgttccccg gacgcaccgg ctcatcgcga
    10741 gccgggatca agctcagtcg tcgtagcctt cgtggcgcga cgcggcggcg tacaggtccg
    10801 cggcgaagcc cggcgaatgc atctcgacgc tgcgggccag ggcgcgcacg tcgttggctt
    10861 cgcggatgcg cacgcggtgg gcgttggcga ccagcgccgg gcgcacggtg gcggcggccg
    10921 gcttgcgggc cgggcgcgag gacgagaaca ggccggcgac cagttggcgg atcgcgcccg
    10981 tgaagatcgg ttggtggccg taggtaccgg aagtgatggc ggtcatggtg tgagtccctg
    11041 caagagcacc ttggtaaggt gcgatggaag gactgtaggt gttttcccgg tgacatgcca
    11101 atgaatgttg gacatccaag atatgcttgg cattcatatc ttgctcgacg gtcaatctcg
    11161 tgaacttccg cacgctggac ctcaacctgc tgcgcgtctt cgacgccgtg atggccgagg
    11221 gcagcctcac gcgcgccgcc gagcgcctcg cgatgaccca gccggcggcc agccacgccc
    11281 tcaagcgcct gcatgtcgcg gtcggcgagg aactgctcca ccgcaccgcc cacggcatgc
    11341 gcccgacggt ccgcgccgag gtgctgtggc cgcaggtgcg cagcgcgctg gcccagttgc
    11401 agggcgcgct gtcgccgccc gacttcgacc cggcgcgcga tgccgccacc ttcagcatcg
    11461 ccatggcgga cgccaccgcc gcgatgctgc tgccggcgct ggtcgtcgcg atcgagaacg
    11521 ccgaggcgat cgccaacgtc cgcatcctgc cgctgaccac gcgcgacccg cgccgcctgg
    11581 tcgaggacgg cgacgccgac ctcgccgtcg gctacttccc caacgccatc gcggccgtca
    11641 tcgcgcaggg ccagga
//
LOCUS       DATCRO010000418         5698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_364454, whole genome shotgun sequence.
ACCESSION   DATCRO010000418 DATCRO010000000
VERSION     DATCRO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364454"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3474)
                     /gene="mfd"
                     /locus_tag="VIN75_11325"
     CDS             complement(<1..3474)
                     /gene="mfd"
                     /locus_tag="VIN75_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY8974801.1"
                     /translation="MQLPSLAPGQRFTLPRPPGAADALLIARLASGRAAQGQLTLVVC
                     AEPADSQRLADELPFFAPHLRVAIFPDWETLPYDQFSPHQDLVSERLATLWRLYRKSQ
                     GLSSANTGAPVAAPKGKGAPDPTRSVDANKEDDEGVDVVLLPASTALTRLAPPSFLAA
                     TTFNFKQKSRLDEAALRAQLTLAGYSHVSSVVSPGEYAVRGGLIDLFPMGSSVPYRVD
                     LFDNEVDSIRTFDPDTQRSLYPVPQVRLLPGREFALDEASRKAFRVRWRERIEGDPTK
                     ARLYKDMGNGIAGGGIEYYLPLFFDETATIFDYLPASATLVLHGAVDEALQRFWSDTR
                     ERYRFLKADPDRPILPPEDIFLKDAQFFDRTRAFAALSLRPPGALAARRTETSGSEDH
                     IRAEPSSPSGGSDGRSEGAVVSWARPLPDVSVERGATAPMARLQKHIDATPYRILLVA
                     DSEGRRETLLDMLRDHQIEPVSVATLAEFEAGDEKIAITAAPISEGFFWFEPDAQRTI
                     QFITENELFATGATPRKRRRGQEQTSSVDALIKDLSELKLGDPVVHASHGIGRYQGLI
                     NIDLGEGPSEFLHLEYADKATLYVPVAQLALISRYTGVSAEEAPLHKLGSGQWDKARR
                     KAAEQVRDAAAELLNLYARRAARDGHSHRFTPHDYEAFATSFGFEETPDQRAAIHAVI
                     QDLVSPKPMDRLVCGDVGFGKTEVALRAAFVAVHGGKQVALLAPTTLLAEQHYQTISS
                     RFGQWPVKVAEMSRFRTAKEIKTAMEGLADGTIDIIVGTHKLLSADVKFKRLGLLIID
                     EEHRFGVRHKEAMKALRAEVDVLTLTATPIPRTLGMALEGLRDLSVIATAPQRRLAIK
                     TFVRNESQGTIREAVLRELKRGGQVYFLHNEVETIPNRLEKLQQLLPEARIAIAHGQM
                     PERELESVMRDFVAQRINVLLCSTIIETGIDVPTANTIVISRADKFGLAQLHQLRGRV
                     GRSHHQAYAYLMVPDVEGLTKQAAQRLDAIQAMEELGSGFYLAMHDLEIRGAGEVLGE
                     NQSGNMMEVGFQLYNDMLAEAVRALKSGQEPDLLSPTGAFGGGIDIKLHAPALLPDAY
                     CGDVQTRLSLYKRLATAERPETLDALLEEITDRFGKLPPQGQALFDTHRLRILARSYG
                     VLKVDANPKVMN"
     gene            3659..4444
                     /gene="ispD"
                     /locus_tag="VIN75_11330"
     CDS             3659..4444
                     /gene="ispD"
                     /locus_tag="VIN75_11330"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY8974802.1"
                     /translation="MTSPQRGDSFTHPHGRRRFFALVPCAGSGSRSGAAGPKQYESLG
                     GRALVLWTLEALGRVAELDGTLVVLSPGDTQFAALAAEGDAEAAAACWRANVGGATRA
                     ETVYAGLHDLLARGALPTDWVLVHDAARCLVHPDSIRRLIAECRDDAVGGLLALPVSD
                     TLKLAGDDSRVAATVPREQIWTAQTPQMFRLGVLRNALRAGLARPELRITDEASAIEA
                     AGFAPRLVRGDFDNLKVTWPEDFALAERLLGSRAKPAFPTAGA"
     gene            4489..4983
                     /gene="ispF"
                     /locus_tag="VIN75_11335"
     CDS             4489..4983
                     /gene="ispF"
                     /locus_tag="VIN75_11335"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8974803.1"
                     /translation="MTIPFRIGEGWDTHQLVEGRPLILGGVTIPHTHGLLGHSDADAL
                     CHAITDALFGAAGLGDIGRHFPDTDAAFKGADSIALLAECVRRVRAAGWEPVNVDSTI
                     VAQKPKLAPHIDAMRARLAETLGLAVDVVNVKAKTAEKMGPVGELKAIETRAVCLLQR
                     IETR"
     gene            complement(4928..>5698)
                     /locus_tag="VIN75_11340"
     CDS             complement(4928..>5698)
                     /locus_tag="VIN75_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974804.1"
                     /translation="ARIDEVVAQSTCARHQWPGRGVAPRGYLLGMGRTYAKAWCELRH
                     PASVAARVAGTNFDAATDALAHYGRAGGSPQDRLRALYALALGEGMRESTGNASAGFN
                     RGVRHASARDAEAGLFQVMPNSLHDSPWLGELYDQYRAQPAWCLREVFMAGTKDRRQP
                     VLGEGPDAQFQRFTKACPAFAVDYATIVLRTDLRKFPPVRHRKAQLVPACVAMFEEVE
                     RVAADDCGVPDAPGARMPAAISASRSAATSTPPASRSP"
BASE COUNT          780 a         1975 c         2070 g          873 t
ORIGIN      
        1 gttcatgacc ttggggttcg cgtccacctt cagcacgccg tagctcctgg ccaggatgcg
       61 cagccggtgc gtgtcgaaca gcgcctggcc ctgcggcggc agcttgccga agcggtcggt
      121 gatctcctcg agcagcgcat ccagcgtctc ggggcgctcc gcggtggcca ggcgcttgta
      181 cagcgacagc cgcgtctgca cgtcgccgca ataggcatcg ggcagcaggg cgggggcgtg
      241 cagcttgatg tcgatgccgc cgccgaacgc gccggtgggc gacagcaggt cgggctcctg
      301 gccggacttg agcgcgcgca cggcctcggc cagcatgtcg ttgtacagct ggaagccgac
      361 ctccatcatg ttgccgctct ggttctcgcc cagcacctcg ccggcgccgc ggatctccag
      421 gtcgtgcatc gcgagataga agcccgagcc gagttcctcc atcgcctgga tggcgtccag
      481 gcgctgcgcg gcctgcttgg tcaggccctc gacgtccggc accatcaggt aggcgtaggc
      541 ctggtggtgc gagcggccga cgcgcccgcg cagctggtgc agctgcgcca ggccgaactt
      601 gtcggcgcgg ctgatgacga tggtgttggc ggtgggcacg tcgatgcccg tctcgatgat
      661 ggtggagcac agcagcacgt tgatgcgctg ggcgacgaag tcgcgcatca ccgattcgag
      721 ctcgcgctcg ggcatctggc cgtgcgcgat cgcgatgcgc gcctcgggca gcaactgctg
      781 cagcttctcg aggcggttgg ggatcgtctc gacctcgttg tgcaggaagt agacctggcc
      841 gccgcgcttg agctcgcgca gcacggcttc gcggatcgtg ccctggctct cgttgcgcac
      901 gaaggtcttg atggccaggc gccgctgcgg cgcggtggcg atcaccgaca ggtcgcgcag
      961 gccttccagc gccatgccca gcgtgcgcgg gatcggggtg gcggtcagcg tgagcacgtc
     1021 gacctccgcg cgcagcgcct tcatggcctc cttgtggcgc acgccgaagc ggtgctcctc
     1081 gtcgatgatc agcaggccca ggcgcttgaa cttgacgtcg gccgacagca gcttgtgcgt
     1141 gccgacgatg atgtcgatcg tgccgtcggc caggccctcc atcgccgtct tgatctcctt
     1201 ggcggtgcga aagcgcgaca tctccgccac cttcaccggc cactgcccga agcggctgga
     1261 gatcgtctgg tagtgctgct ccgccagcag cgtggtgggc gccagcagcg cgacctgctt
     1321 gccgccgtgc acggccacga acgcggcgcg cagcgcgact tccgtcttgc cgaagccgac
     1381 gtcgccgcac accagccggt ccatcggctt ggggctgacc aggtcctgga tgacggcgtg
     1441 gatcgcggca cgctggtcgg gcgtctcctc gaagccgaag ctggtggcga aggcttcgta
     1501 gtcgtgcggc gtgaaccggt gcgagtggcc gtcgcgggcg gcgcggcggg cgtacaggtt
     1561 cagcagctcg gcggcggcgt cgcgcacctg ctcggcggcc ttgcggcgcg ccttgtccca
     1621 ctggcccgag cccagcttgt gcagcggcgc ctcctccgcc gacacgcccg tgtagcggct
     1681 gatcagcgcg agctgcgcga cgggcacgta cagcgtggcc ttgtcggcgt actccaggtg
     1741 caggaactcg ctcgggccct cgcccaggtc gatgttgatc agcccctggt agcggccgat
     1801 gccgtggctc gcgtgcacca ccgggtcgcc cagcttcagc tcggacagat ccttgatgag
     1861 cgcgtcgacg ctgctcgtct gctcctggcc gcggcggcgc ttgcgcggcg tggcccccgt
     1921 ggcgaacagc tcgttctcgg tgatgaactg gatcgtgcgc tgcgcgtcgg gctcgaacca
     1981 gaagaagcct tcgctgattg gcgcggcggt gatcgcgatc ttctcgtcgc ccgcctcgaa
     2041 ctcggccagc gtggcgacgc tcacgggttc gatctggtgg tcgcgcagca tgtccagcag
     2101 cgtctcgcgc cggccctcgc tgtcggcgac cagcaggatc cggtacggcg tggcatcgat
     2161 gtgcttctgc agtcgcgcca tcggcgccgt cgcgccgcgt tcgacactga cgtcgggcag
     2221 cggccgtgcc caagacacga cggccccctc gctcctcccg tcgctgcccc ccgaggggga
     2281 gctcggttcg gctcggatgt gatcctcgct tcccgaggtt tcggtgcggc gcgcggccag
     2341 cgcgcccggc ggcctgagcg acagcgccgc gaacgcccgc gtgcggtcga agaactgcgc
     2401 gtccttcagg aagatgtcct ccggcggcag gatgggccgg tcggggtcgg ccttcaggaa
     2461 ccgataccgc tcccgggtgt cggaccagaa gcgctgcagc gcctcgtcga cggcgccgtg
     2521 cagcaccagc gtcgccgagg cgggcaggta gtcgaagatc gtcgcggtct cgtcgaagaa
     2581 cagcggcagg tagtactcga tgccgccgcc ggcgatgccg ttgcccatgt ccttgtacag
     2641 gcgcgccttg gtggggtcgc cctcgatgcg ttcgcgccag cgcacgcgga acgccttgcg
     2701 cgaggcctcg tcgagcgcga actcgcggcc cggcagcagg cgcacctgcg gcacggggta
     2761 caggctgcgc tgggtgtcgg ggtcgaaggt gcggatcgag tcgacctcgt tgtcgaacag
     2821 gtcgacgcgg tagggcacgc tggagcccat cgggaacagg tcgatcaggc cgccgcgcac
     2881 ggcgtactcg cccggcgaca ccacggagct gacgtgcgag tagcccgcca gcgtcagctg
     2941 cgcgcgcagc gcggcctcgt ccagccgcga tttctgcttg aagttgaacg tggtggccgc
     3001 caggaacgac ggcggcgcga gacgcgtcag cgcggtgctg gccggcagca gaacgacgtc
     3061 gacgccctcg tcgtcctcct tgttggcgtc gacgctgcgc gtggggtcgg gcgcgccctt
     3121 gcccttgggt gccgcgacgg gcgcgccggt gttggcgctc gacagtccct ggctcttgcg
     3181 gtacagccgc cacagcgtgg ccaggcgttc ggagacgagg tcctggtgcg gcgagaactg
     3241 gtcgtagggc agcgtctccc agtccgggaa gatggccacg cgcaggtgcg gcgcgaagaa
     3301 cggcagctcg tcggccaggc gctggctgtc ggccggctcc gcgcatacga ccagcgtcag
     3361 ctggccctgc gccgcgcgcc cggaggccag gcgggcgatc agcagcgcgt cggccgcgcc
     3421 gggcgggcgg ggcagggtga agcgttgtcc gggagcgagc gagggcagtt gcatgacggg
     3481 agcagaaatg acgacacgcc ttggacgcgc cttcctgtcg ggtcaggtct tgaatcgtgc
     3541 actcgggcac gggcgaaagt gcacgattcg agacctgaca cccggcaaga agggcgccgg
     3601 caaggcgtgg tgcccgtgaa agtcgagcag ggagcgcgac gattctagaa tctccaccat
     3661 gaccagtccc cagcgcggtg attccttcac ccatccgcac ggccgccggc gattcttcgc
     3721 gctggtgccc tgcgccggtt cgggctcccg ctcgggtgcg gccggcccca agcagtacga
     3781 atcgctcggc ggccgagcgc tggtgctgtg gacgctggag gcattgggcc gggtggccga
     3841 actggatggc acgctggtcg tgctgtcccc cggcgacacg cagttcgccg cgctggccgc
     3901 cgagggcgac gccgaggcgg cggccgcgtg ctggcgcgcc aacgtcggcg gcgccacgcg
     3961 cgccgagacg gtctacgcgg gcctgcacga cctgctcgcg cgcggcgcgc tgcccaccga
     4021 ctgggtgctg gtgcacgacg cggcgcgctg tctggtccat cccgattcca tccgccgcct
     4081 gatcgccgag tgccgcgacg acgccgtcgg cggcctgctg gcattgcctg tgtcagatac
     4141 cttgaagctc gccggcgacg attcgcgcgt cgccgccacc gtcccgcgcg agcagatctg
     4201 gaccgcgcag acgccacaga tgttccgcct cggcgtcctg cgcaatgcgc tgcgcgccgg
     4261 cttggcgcga cccgaactgc ggatcaccga cgaggccagc gccatcgagg ccgcgggctt
     4321 cgcgccgcgc ctggtgcgcg gcgatttcga caacctcaag gtgacctggc ccgaggactt
     4381 cgcgctcgcg gagcggctgt tgggatcgcg ggcgaagccc gcattcccga cagccggcgc
     4441 gtagcgacgg attccgagcc gcgcgccgca cgcaccgaaa gcccacccat gaccattccg
     4501 tttcgcatcg gcgaaggctg ggacacgcac cagctcgtcg agggccgccc gctgatcctg
     4561 ggcggcgtca cgatcccgca cacgcacggc ctgctcgggc attccgacgc cgacgcgctg
     4621 tgccacgcca tcaccgacgc cctgttcggc gccgccggcc tgggcgacat cggccgccac
     4681 ttccctgaca ccgacgccgc cttcaagggc gccgactcga tcgccctgct ggccgaatgc
     4741 gtgcgccgcg tgcgcgcggc cggctgggag ccggtcaacg tcgactccac catcgttgcg
     4801 cagaagccca agctggcgcc gcacatcgac gcgatgcgcg cgcgcctggc cgagacgctg
     4861 ggcctggcgg tcgacgtcgt caacgtgaag gccaagacgg ccgagaagat ggggccggtg
     4921 ggcgagctca aggcgatcga gacgcgggcg gtgtgcttgt tgcagcggat cgagacgcgc
     4981 tgatcgccgc gggcatgcgg gcacctggcg catccgggac gccgcagtcg tccgcagcca
     5041 cgcgctcgac ctcctcgaac atcgccacgc aggccggcac cagctgcgcc ttgcgatggc
     5101 gcaccggcgg gaacttgcgc aggtcggtgc gcagcacgat cgtcgcgtag tcgacggcga
     5161 aggcggggca ggccttggtg aaacgctgga attgggcatc ggggccttcg ccgagcacgg
     5221 gttggcggcg atccttcgtg cccgccatga acacctcgcg caggcaccaa gccggctgtg
     5281 cacggtactg gtcgtagagc tcacccagcc aaggcgagtc gtgcagcgag ttgggcatga
     5341 cctggaacag gccggcctcg gcgtcccggg ccgacgcgtg ccgcacgccg cggttgaagc
     5401 cggccgacgc gttgccggtg ctctcgcgca tgccctcgcc gagcgccagg gcgtagagcg
     5461 cgcgcaaccg gtcctgcggc gagccgccgg cgcggccgta gtgggcgagg gcgtcggtgg
     5521 ccgcatcgaa attcgttccg gccacgcgtg cggcgaccga ggccgggtgc cgcagctcgc
     5581 accaggcctt cgcataggtg cggcccatgc ccagcaggta gccgcgggga gcgacgccgc
     5641 gtccgggcca ttggtggcgg gcgcaggtcg actgcgccac gacctcgtcg atccgcgc
//
LOCUS       DATCRO010000419         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_668406, whole genome shotgun sequence.
ACCESSION   DATCRO010000419 DATCRO010000000
VERSION     DATCRO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668406"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VIN75_11345"
     CDS             <1..409
                     /locus_tag="VIN75_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8974805.1"
                     /translation="SFPDMSSIHKTLPIAALAFALAGTAFSEGTDIEVDGDLMRGIDD
                     TAKSLDSDVAMKDGTAAAADARSLVETFARVESHYGEKPDTADAAGFAHRTQELAAQA
                     LKAIEAKDFDAAGEAVNQLTRSCKNCHEKYKKD"
     gene            411..821
                     /locus_tag="VIN75_11350"
     CDS             411..821
                     /locus_tag="VIN75_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974806.1"
                     /translation="MGPTPRPRATLAAVAALALAATAATVARAASVLDFDVWMRAIDK
                     HSVEVQRNIDARRTDAAIADAQELVRLYGLMEAYFVADGQKADAVDMSRSGKALATAI
                     PAALGAQDYEAASRAAVDLAHACNDCHDVHKPFQ"
     gene            847..1317
                     /locus_tag="VIN75_11355"
     CDS             847..1317
                     /locus_tag="VIN75_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8974807.1"
                     /translation="MNTTRTILLLAAALAAAGTALAQQAPRPENLIKWRQSAFQVVAW
                     NSGRIKASLDGGYNKDQVLRSANAIAAIANSGLGSLFPPGTETGKGWHDTSVKADAFT
                     NTARFAEHGGDFARESNELVRLVSAGSDAGAVKDQFGKLQKTCKSCHDDFRNAN"
     gene            1328..2062
                     /locus_tag="VIN75_11360"
     CDS             1328..2062
                     /locus_tag="VIN75_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HEY8974808.1"
                     /translation="MKIRSESTALAACTVLAVAATLSTALAAHAQSASSPAPAASAPR
                     PLPKDFSFFLTSEPIGQGGNLGGLAGADAHCQKLAAAVGFGHRTWRAYLSTQAADGAP
                     AVNARDRIGAGPWINARGAVIAKDIAQLHGDTIELAQLGNNLSKATVFTETGETRPGF
                     GDKPNVHDVITGSQPDGRAYADTADHTCRNYTSSASDASTQLGHFDRAGGGNTSWNSA
                     HKSRGCSQDNLVSTGGAGLLYCFAAG"
     gene            complement(2322..3230)
                     /locus_tag="VIN75_11365"
     CDS             complement(2322..3230)
                     /locus_tag="VIN75_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8974809.1"
                     /translation="MKRTLASLAAVLALANASSARAQAAPDFSKVEIKATQLAPDFWT
                     LEGQGGTISVLTGNDGILLVDSQFAPLTDKLVAAIRRFSDKPIRFLVDTHVHPDHVGG
                     NANFARLGATIFARDQLRWRLEHPNAAADGTPGKPAPDEALPVVTYNASLSIHVDGED
                     VRLLPVVAAHTDGDTVVSFPGHDIIAAGDIFRSVGYPYVDLASGGSLAGILEGLSEIV
                     DRSGPATKIVPGHGPIVDRNAVLAQRDLILAVRAKVLTLIEQGRTVDEAVAAHPTAEF
                     DARVPQSAQTADRFVRWVYAEVAANR"
     gene            complement(3298..4047)
                     /locus_tag="VIN75_11370"
     CDS             complement(3298..4047)
                     /locus_tag="VIN75_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8974810.1"
                     /translation="MSAVLDPHGAALRAPLPIPARPAPAPAEVLPFPRRARPSARAKA
                     QVKGRGTGIAVVAGIHVLFGWALVSGLAQHVVDAVRKPIDMAIIAETPPPPPPPPKVE
                     KVVERPKAATPPPPAYVPPPEVVPPPAPPAPTIVATAAEPPKEPVVAAPPAPAPAPPP
                     AVVRQEVSLACPGYQSALATVLEDAIDRVGVTGTVRTRLTVRGGQVTDVAFLSGPKEY
                     TKYVQAAVKRMHCSAGGADEVQVPLDVNFAR"
     gene            complement(4055..4492)
                     /locus_tag="VIN75_11375"
     CDS             complement(4055..4492)
                     /locus_tag="VIN75_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8974811.1"
                     /translation="MSSKLRARRRAPADDLPVADINTTPLIDVMLVLLVMIIITLPVQ
                     LHAVNLRLPTGDPPPAERKPEVVQIDIAPGGTLAWNGAPVADAAALKALLAAAAAQAE
                     PPEIHVRPDQSARYDAFAAVMVAANQAGLTKVGVTGSEQFIPK"
     gene            complement(4498..4917)
                     /locus_tag="VIN75_11380"
     CDS             complement(4498..4917)
                     /locus_tag="VIN75_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8974812.1"
                     /translation="MNLPAAGGEDQAVAAINTTPLVDVMLVLLIIFLITIPVVNFSVP
                     VALPKQRNQVRESQPRDIVISVDAHGATFWFDSRVAGDEALADKLKAVSLQDPQPEIH
                     IRGDARSDYAPVGRILYACQRAGIRHVGFITEPPPKG"
BASE COUNT          769 a         1855 c         1793 g          675 t
ORIGIN      
        1 atcgttcccc gacatgagtt ccatccacaa gacccttccc atcgccgccc tggcgttcgc
       61 gctggccggt accgcgttca gcgaaggcac cgacatcgag gtcgatggcg acctgatgcg
      121 cggcatcgac gacaccgcca agagcctcga ttccgacgtc gcgatgaagg acggcacggc
      181 cgctgcggcc gacgcccgct cgctcgtcga aaccttcgcc cgcgtcgagt cgcactacgg
      241 cgagaagccc gacaccgccg acgcggccgg cttcgcgcac cgcacgcagg aactggccgc
      301 gcaggcgctc aaggccatcg aggccaagga cttcgacgcc gccggcgagg ccgtcaacca
      361 gctcacgcgc tcgtgcaaga actgccacga gaaatacaag aaggactgac atgggaccca
      421 cgccgcgccc ccgcgcgacg ctcgccgccg tcgcggcgct cgcgctagcc gccaccgctg
      481 ccaccgtggc gcgcgccgcc agcgtgctcg acttcgacgt ctggatgcgt gccatcgaca
      541 agcacagcgt cgaggtacag agaaacatcg acgcgcgcag gacggatgcc gccatcgccg
      601 acgcgcagga gctcgtgcgc ctgtacgggc tgatggaggc gtatttcgtc gccgacggcc
      661 agaaggccga cgccgtcgac atgtcgcgca gcggcaaggc gctggccact gcgatccccg
      721 cggcactcgg cgcgcaggac tacgaggccg cgtccagggc cgccgtcgat ctcgcgcacg
      781 cctgcaacga ctgccacgac gtccacaagc cattccagtg attcccgacg acacgacgct
      841 ttcgacatga acacgactcg cacgatcctc ctgctggccg ccgcgctcgc ggccgcgggt
      901 accgcgctcg cacagcaggc gcccaggccc gagaacctca tcaagtggcg ccagtcggcg
      961 ttccaggtgg tcgcctggaa ctccggccgc atcaaggcca gcctcgacgg cggctacaac
     1021 aaggaccagg tgctgcgttc cgccaacgcg atcgccgcca tcgccaactc gggcctgggc
     1081 agcctgtttc cccccggcac cgagacgggc aagggctggc acgacacctc ggtcaaggcc
     1141 gacgccttca ccaacacggc caggttcgcc gagcacggcg gcgacttcgc cagggagtcg
     1201 aacgaactgg tgcgcctggt ctcggccggc agcgacgcgg gcgccgtcaa ggaccagttc
     1261 ggcaagttgc agaagacctg caagtcctgc cacgacgact tccgcaacgc caactgaggc
     1321 cacgaccatg aagatccgct ccgaatcgac cgcgctcgcc gcctgcaccg tgctggccgt
     1381 cgccgccacg ctgtccaccg cgctcgcggc gcacgcccag tcggcgtcgt cgcccgctcc
     1441 ggccgcctcc gcgccgcgcc ccctgcccaa ggacttcagc ttcttcctga ccagcgagcc
     1501 gatcggccag ggcggcaacc tcggcggcct ggccggcgcc gacgcgcact gccagaagct
     1561 ggccgccgcg gtcggcttcg gccatcgcac ctggcgcgcc tatctgagca cgcaggcggc
     1621 cgacggcgcg ccggccgtga acgcgcgcga ccgcatcggc gcgggtccgt ggatcaacgc
     1681 gcgcggtgcc gtcatcgcca aggacatcgc ccagctgcac ggcgacacga tcgagctggc
     1741 gcagctgggc aacaacctca gcaaggccac ggtcttcacg gagaccggcg agacgcgccc
     1801 gggattcggc gacaagccca acgtgcacga cgtcatcacc ggctcgcagc ccgatggccg
     1861 cgcgtatgcc gacaccgccg accacacctg ccgcaactac acgagcagcg cgagcgacgc
     1921 ctcgacccag ctgggccatt tcgaccgcgc cggcggcggc aacacctcgt ggaactcggc
     1981 gcacaagagc cgcggctgca gccaggacaa cctggtcagc acgggcggcg cggggctgct
     2041 gtactgcttc gcggcgggct gaccaggggt ctggcctctc ctcgcttcgc gccacgatgt
     2101 cgatcgcagc gacatccctg ccgcgaaggg aggacaggcc tgaccccgat ccccgggcga
     2161 aacggcgtca ggcctgtcct cgaatcgcgg aagaaacctc gcccctggac gacgaaaccg
     2221 cgcgattcga ggagaggcca gaccccttcg gcagcgagtc ggcgcgcgga gcgagaggat
     2281 ccggcagcct ctcgagagtg ccggcccctt cggcggtccg gtcagcggtt cgccgccacc
     2341 tccgcataca cccaccgcac gaaccgatcc gccgtctgcg ccgactgcgg cacccgcgcg
     2401 tcgaactccg ccgtcggatg cgcggcaacg gcttcgtcga ccgtcctgcc ctgctcgatc
     2461 agcgtgagca ccttggcacg cacggccagg atcaggtcgc gctgcgcgag caccgcgttg
     2521 cgatcgacga tggggccgtg gcctggaacg atcttcgtgg ccgggcccga gcggtcgacg
     2581 atctcgctca ggccctcgag gatgcccgcc agcgagccgc cgctggcaag atcgacgtac
     2641 gggtagccga cgctgcggaa gatgtcgccc gccgcgatga tgtcgtgacc ggggaacgag
     2701 acgaccgtgt cgccgtcggt gtgcgccgcg acgaccggca gcaggcgcac gtcctcgccg
     2761 tcgacgtgga tcgacagcga ggcgttgtac gtcaccaccg gcagcgcctc gtcgggcgcg
     2821 ggcttgccgg gcgtgccgtc ggccgccgcg ttcggatgct ccaggcgcca gcgcagctgg
     2881 tcgcgcgcga agatcgtcgc gcccagccgc gcgaagttcg cgttgccgcc gacatggtcc
     2941 ggatgcacat gggtgtcgac caggaagcgg atcggcttgt ccgagaacct gcggatcgcg
     3001 gcgacgagct tgtcggtcag cggcgcgaac tgcgaatcga ccagcaggat gccgtcgttg
     3061 ccggtcagca cgctgatcgt gccgccctgg ccttccagcg tccagaagtc gggcgcgagc
     3121 tgggtcgcct tgatctcgac cttgctgaag tccggcgccg cttgcgcgcg ggccgacgag
     3181 gcgttggcga gcgcgaggac cgcggcgagc gaagcgaggg tgcgtttcat gtcggtgtcg
     3241 gtagacaggc gggacggcca tcctaagggc cgcccacgcc cgcgagctcg gcagggtcta
     3301 tcgcgcgaag ttcacgtcga gcggcacctg cacctcgtcc gcgccgcccg cggagcagtg
     3361 catgcgcttg accgcggcct gcacgtactt cgtgtactcc ttgggcccgg acaggaacgc
     3421 gacgtcggtc acctggccgc cacgcaccgt cagccgcgtg cgcaccgtgc ccgtgacacc
     3481 gacgcggtcg atcgcgtcct ccagcaccgt ggccagggcg gactggtagc cggggcaggc
     3541 gaggctcacc tcctggcgca cgaccgccgg cggcggcgcg ggggcggggg ccggcggcgc
     3601 ggcgaccacc ggctccttcg gcggctcggc agcggtggcc acgatcgtcg gcgcgggcgg
     3661 cgccggcggc ggaaccacct cgggcggggg cacgtaggcc ggcggcggcg gcgtcgcggc
     3721 cttcgggcgc tcgaccacct tctcgacctt gggcggcggc ggaggcggcg gcggggtctc
     3781 ggcgatgatg gccatgtcga tgggcttgcg caccgcgtcg acgacgtgct gcgcgagccc
     3841 cgacaccagt gcccagccga acagcacgtg gatgccggcc accacggcga tcccggtgcc
     3901 gcggcccttg acctgcgcct tcgcacgcgc ggacgggcgc gcccgccgcg ggaacggcag
     3961 tacttcggcc ggtgccggcg cgggccgggc ggggatgggc aggggcgcgc gcagcgcggc
     4021 gccgtgcgga tcgagtacag cactcatggg gtcctcactt cggtatgaat tgctcgctgc
     4081 cggtgacgcc gaccttggtc agtccggcct ggttggcggc caccatcacg gccgcgaacg
     4141 cgtcgtacct ggcggactgg tcgggtcgca catggatctc cggcggctcg gcctgcgccg
     4201 cggcggcggc cagcagcgcc ttgagcgccg ccgcgtcggc gacgggcgcg ccgttccatg
     4261 ccagcgtgcc gcccggcgcg atgtcgatct gcaccacctc cggcttgcgc tcggccggcg
     4321 gcgggtcgcc ggtgggcagg cgcaggttga cggcgtgcag ctgcacgggg agcgtgatga
     4381 tgatcatcac cagcagcacg agcatcacgt cgatcagcgg cgtcgtgttg atgtcggcca
     4441 ccggcaggtc gtcggcgggg gcgcggcggc gcgcgcggag cttgctggac atggcgctca
     4501 tcccttgggc ggcggttcgg tgatgaagcc gacgtgccgg attccggcgc gctggcatgc
     4561 gtagaggatg cggcccaccg gcgcgtagtc gctgcgggcg tcgccgcgga tgtggatctc
     4621 gggctgcggg tcctgcagcg acacggcctt gagcttgtcg gccagggcct cgtcgccggc
     4681 gacccggctg tcgaaccaga acgtggcgcc gtgcgcgtcg acggagatca cgatgtccct
     4741 gggctggctc tcgcgcacct ggttgcgctg cttgggcagg gcgacgggca cggagaagtt
     4801 caccaccggg atcgtgatca ggaagatgat cagcagcacc agcatcacgt cgaccagcgg
     4861 cgtggtgttg atggccgcga ccgcctggtc ctcgcctccg gcggcaggca ggttcatcgc
     4921 catgtcaggc cgccgccgtg gccgtcgcgc ccgcgtgggc gcccgcgctc gcggacgcgg
     4981 gcgcgcccga gcccagcagc acgccgtgca gctcgcccga gaagtcgcgc acctcgtcca
     5041 gcaccgcgct gttgcggcgc aggaggaagt tgtagcccag cacggcgggc ac
//
LOCUS       DATCRO010000420         6218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_949246, whole genome shotgun sequence.
ACCESSION   DATCRO010000420 DATCRO010000000
VERSION     DATCRO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949246"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VIN75_11385"
     CDS             <1..754
                     /locus_tag="VIN75_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8974813.1"
                     /translation="DVLLGTVNTALLLTSSAAVALAVACEKDADQRRWTARLLVLTAA
                     TLAHPELDEKQESGTGKLGMWVFLASEVLFFGGLFVAYLYGRTHFPAGWAEGSRRTDV
                     VLGTVNTAVLLTSSACVALAVACEEDERQRRWTARLLSLTAALGVVFLVIKGLEWHDE
                     WREHLMPGTDFELRIAGAQQFFALYFLTTGLHGVHMICGIVALCILARGRSRGRAWAG
                     AGELEVAALYWHFVDIVWIFLYPLLYLMDRHG"
     gene            747..1115
                     /locus_tag="VIN75_11390"
     CDS             747..1115
                     /locus_tag="VIN75_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY8974814.1"
                     /translation="MADDPAASPRFARERRKLALTWIALLLLMLASLGSSYVKLGAWN
                     MVAGLVIAVIKASLVAWLFMRLRESGALVRLAAVAGLGAWCILLVLGGVDYSTRTVTP
                     TEVQRPQQLPPVHAAASEPR"
     gene            1164..2255
                     /locus_tag="VIN75_11395"
     CDS             1164..2255
                     /locus_tag="VIN75_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HEY8974815.1"
                     /translation="MPTLLRRELALVLAALALAVAWPLEHAILGAGQGASLAASIALI
                     AAILFASVRVAHHAETLAERLGEPYGTMVLTLSAVLVEVVILAILMQESPSPTLARDT
                     IYSAVMLDINALLGLSALLGGLRHGEQPYNVDSGNTYVVMILTGLGVAMVLPAFIPVA
                     HWAAYSIFTIVVMVALYGMFLKLQTGRHSYFFSYSYAQKAPADEASDDGHVEGGLAAP
                     LLGIGAGIVLIGLLSEVMARTLDTGLQGIGAPPILAPLIVATISASPEILTALRAALS
                     NRMQPVVNIALGASLSTVVLTVPVIEALALATRHPITMALTAPQMVMTLLTLLVATIN
                     IHDGETNAIEGLTHFVLFAAFLMLSAIGL"
     gene            2353..3702
                     /locus_tag="VIN75_11400"
     CDS             2353..3702
                     /locus_tag="VIN75_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8974816.1"
                     /translation="MKVAAFTSSSQPRKHAGDGADIDVSSLIERAQRGAFGYFETATD
                     PASGLVLDSTQPDSPCSISGLGMALTAYPIAVERGWMSRLQAQAITLRTLRFLAEADM
                     SGTPHSTGYRGFYFHFLDMKTGTRAWRSELSSIDTSFLVAGALAAAGFFDRDNGHEAE
                     IRGLARLLADRVDWAWMCNCDGAICHGWKPERGFLRYHWVGYSEGLLMYALALGSEHH
                     RIATRAWDAWLAGYRWRRIYGHEHLAAGPLFIHQYSHMWIDLRGIQDAFMRSKGIDYF
                     ENSRRATLVQREYAIRNPKHFRHYCENCWGWTACDGPGPRQVTVDGRARRLLGYAARG
                     APYGPDDGTVAPWACAASLPFAPDVVIPTMQHIARLTREYPDYMPGCSFNPTWGGGKD
                     APGWVSPWRFALNSGPVVAMIENYRSGLPWELMKREPTIRRGLARADFRGGWLSARP"
     gene            complement(3699..4895)
                     /locus_tag="VIN75_11405"
     CDS             complement(3699..4895)
                     /locus_tag="VIN75_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974817.1"
                     /translation="MKTDCVIAGGGPAGMMMGLLLARAGVDVVVLEKHADFLRDFRGD
                     TIHPSTLDVMHELDLLDEFLRRPHDELREVAVRVGADQAQIADFRHVPTHCKFLVMMP
                     QWDFLDFLADHARRLPSFHLVMKAEATTLIREDGRVAGLRARTPEGELEIRAALVVGA
                     DGRHSTIRAQAGLETLDLGAPMDVLWLRVSRVPGDPPDLFGQVDAGRMLVMIARNDYW
                     QCALLIPKGSDAQVRARGIEALHESILGLSPWLGDRVKELVDWDQVKLLAVTVDRLRD
                     WARPGVLCIGDAAHAMSPIGGVGINLAVQDAVAAARLLRRPLRERRLQLDDLLRVQRR
                     REWPMKVVQGMQLRMQDRLIRPILAASGPVRMPWFLRLFGRFALLRRIPAWFIGVGVR
                     PEHVER"
     gene            5261..5773
                     /locus_tag="VIN75_11410"
     CDS             5261..5773
                     /locus_tag="VIN75_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8974818.1"
                     /translation="MAARPKLVAVVSDPPVDHDQAAIAVLRQFRQVFNAVKSHFQQVE
                     KTVGMGGAQVWALSVVREQPGIGVGALARAMSIHQSTASNLVRTLIDRDMLVAVKQGA
                     DRRAVQLNLLPAGARVLRNAPGPFAGLLPDALKALPPETLARLQSDLDQVIVRIAADE
                     AAAHVPLAGL"
     gene            5817..6176
                     /locus_tag="VIN75_11415"
     CDS             5817..6176
                     /locus_tag="VIN75_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEY8974819.1"
                     /translation="MTLPTLTATSDCIAACTACAAACDQCFAGCLKEEDVAMMARCVA
                     LDVDCAAVCRLAAGVMLRDSPLAGPVCALCAQVCDACADECAQHEHEHCQLCAQACRA
                     CAESCRAMATLAPDSEH"
BASE COUNT          856 a         2282 c         2136 g          944 t
ORIGIN      
        1 cgacgtgctg ctgggcaccg tcaacaccgc gttgctgctc accagcagcg cggccgtcgc
       61 actggccgtg gcctgcgaga aggacgcgga ccagcgccgc tggacggcgc ggctgctcgt
      121 gctgaccgcc gcgacgctcg cccacccgga actcgacgag aagcaggagt ccgggaccgg
      181 caagctgggc atgtgggtgt tcctggccag cgaggtgctg ttcttcggcg gcctgttcgt
      241 cgcctacctc tacggccgca cgcacttccc ggccggctgg gccgagggct cgcgccgcac
      301 cgatgtcgtg ctgggcaccg tcaacaccgc ggtgctgctg accagcagcg cgtgcgtcgc
      361 gctggccgtg gcctgcgagg aagacgagcg ccagcgccgc tggaccgcgc ggctgctgtc
      421 gctcaccgca gcgctgggcg tcgtgttcct cgtgatcaag gggctcgaat ggcacgacga
      481 atggcgcgag cacctcatgc cgggcacgga cttcgagctg cgcatcgccg gcgcgcaaca
      541 gttcttcgcg ctgtacttcc tcacgacggg gctgcacggc gtgcacatga tctgcggcat
      601 cgtcgcgctg tgcatcctcg cgcgcggacg ctcgcgcggg cgggcgtggg ccggcgcggg
      661 cgagctcgag gtcgcggcgc tgtactggca cttcgtcgac atcgtctgga tcttcctgta
      721 cccgctgctg tacctgatgg accgccatgg ctgacgaccc cgccgcctcg ccccggttcg
      781 cgcgcgaacg ccgcaagctc gcgctcacgt ggatcgcgct gctgctgctg atgctcgcca
      841 gcctgggcag ctcgtacgtg aagctgggcg cctggaacat ggtggcgggc ctggtgatcg
      901 cggtcatcaa ggcgagcctc gtcgcgtggc tgttcatgcg gctgcgcgag tcgggcgcgc
      961 tggtccggct ggcggccgtc gccgggctgg gcgcgtggtg catcctgctc gtgctggggg
     1021 gcgtcgacta ctcgacgcgc acggtgacgc ccaccgaggt gcagcgcccg cagcagctgc
     1081 cgccggtgca cgccgccgcc tcggagccgc gctgaccggt gcgtccccta tcatcggcgc
     1141 tccgcaacct gaacctgacc tgaatgccga ctcttctccg ccgcgaactg gccctggtgc
     1201 tcgcggcgct cgcgctcgcc gtcgcctggc cgctggaaca cgccatcctg ggcgcgggcc
     1261 agggcgcgtc gctggccgcc tccatcgccc tgatcgcggc catcctgttc gcctcggtgc
     1321 gcgtcgccca ccatgccgag acgctggccg aacggctggg cgagccctac ggcaccatgg
     1381 tgctgacgct cagcgccgtg ctggtcgagg tggtgatcct cgcgatcctg atgcaggagt
     1441 cgccgtcgcc cacgctggcg cgcgacacca tctattcggc cgtgatgctg gacatcaacg
     1501 cgctgctggg cctgtcggcg ctgctgggcg gcctgcgcca cggcgagcag ccgtacaacg
     1561 tcgactcggg caacacctac gtcgtgatga tcctgacggg cctgggcgtg gcgatggtgc
     1621 tgccggcctt catcccggtc gcgcactggg ccgcctactc gatcttcacg atcgtcgtca
     1681 tggtcgcgct gtacggcatg ttcctgaagc tgcagacggg ccgccacagc tacttcttca
     1741 gctactcgta cgcccagaag gcgccggccg acgaggcgtc cgacgacggg cacgtcgagg
     1801 gcgggctcgc cgcgccgttg ctgggcatcg gcgcgggcat cgtgctgatc ggcctgctct
     1861 cggaggtgat ggccaggacg ctggataccg gccttcaagg catcggcgcg ccgcccatcc
     1921 tcgcgccgct gatcgtcgcg acgatctcgg ccagccccga gatcctgacc gccctgcgcg
     1981 cggcgctgtc caaccgcatg cagccggtgg tcaacatcgc gctcggcgcg tcgctgtcga
     2041 ccgtggtgct gaccgtgccg gtgatcgagg cgctggcgct cgccacgcgc cacccgatca
     2101 cgatggcgct gaccgccccg cagatggtga tgacgctgct cacgctgctg gtggccacca
     2161 tcaacatcca cgacggcgag accaacgcca tcgagggcct gacccacttc gtgctgttcg
     2221 cggccttcct gatgctctcg gcgatcggcc tgtagcccgc gtgcccggcg gcacgccggc
     2281 cgggcgcgcc gctcgcaatc cgtccccgcg gacggcaccg gcatgcgctt tccgctaggc
     2341 tggcccgcag ccatgaaggt cgccgcattc acctcgtcca gccagccgcg caagcatgcg
     2401 ggcgacggcg ccgacatcga cgtctcgagc ctgatcgagc gcgcccagcg tggcgcgttc
     2461 ggctacttcg agaccgcgac cgacccggcc agcggcctgg tgctcgactc cacacagccc
     2521 gacagcccct gcagcatcag cgggctgggc atggcgttga cggcctaccc gatcgcggtc
     2581 gagcgcggct ggatgagccg gctgcaggcg caggcgatca cgctgcgcac gctgcgtttc
     2641 ctggccgagg cggacatgtc cggcacgccg cactcgaccg gctaccgcgg cttctacttc
     2701 cacttcctgg acatgaagac cggcacgcgc gcctggcgca gcgaactgtc cagcatcgac
     2761 acctcgttcc tggtcgccgg cgccctggcc gccgccggct tcttcgaccg cgacaacggc
     2821 cacgaggccg agatccgcgg cctcgcccgc ctgctggccg accgcgtcga ctgggcctgg
     2881 atgtgcaact gcgacggcgc gatctgccac ggctggaagc ccgagcgcgg cttcctgcgc
     2941 taccactggg tgggctacag cgaggggctg ctgatgtacg cgctggcgct gggctccgaa
     3001 caccaccgca tcgcgaccag ggcctgggac gcgtggctcg cgggctaccg ctggcgccgc
     3061 atctacggcc acgagcacct ggccgccggc ccgctgttca tccaccagta ctcgcacatg
     3121 tggatcgacc tgcgcggcat ccaggacgcg ttcatgcgct ccaagggcat cgactacttc
     3181 gagaacagcc gccgcgcgac gctggtgcag cgcgagtacg cgatccgcaa ccccaagcac
     3241 ttcaggcact actgcgagaa ctgctggggc tggacggcct gcgacggccc ggggccgcgc
     3301 caggtgacgg tggacggacg cgcgcgtcgc ctgctgggct acgccgcgcg cggcgcaccc
     3361 tacggcccgg acgacggcac cgtcgcgccg tgggcctgcg cggcctcgct gccgttcgcg
     3421 cccgacgtcg tcattcccac gatgcagcac atcgcgcgcc tgacgcgcga gtacccggac
     3481 tacatgcccg gctgctcgtt caacccgacc tggggcggcg gcaaggatgc gcccggctgg
     3541 gtctcgccgt ggcgcttcgc gctcaacagc ggaccggtgg tcgcgatgat cgagaactac
     3601 cgcagcggcc tgccgtggga actgatgaag cgcgagccga cgatccgccg cggcctggcg
     3661 cgcgccgact tccgcggcgg ctggctgtcc gcgcgaccct agcgttcgac gtgctcgggt
     3721 cgcacgccca cgccgatgaa ccaggccggg atgcggcgca gcaacgcgaa tcgcccgaac
     3781 agccgcagga accacggcat gcgcaccggg ccgctcgcgg cgaggatggg ccggatcagc
     3841 cggtcctgca tccgcagttg catgccctgc accaccttca tcggccactc gcggcgccgc
     3901 tgcacgcgca gcaggtcgtc cagctgcagc cgccgctcgc gcagcggccg gcgcagcagc
     3961 cgcgccgcag ccaccgcgtc ctgcacggcc aggttgatgc ccacgccgcc gatgggcgac
     4021 atcgcgtgcg ccgcgtcgcc gatgcacagc acgccgggcc gggcccagtc gcgcaggcgg
     4081 tcgaccgtca ccgccagcag cttgacctgg tcccagtcga ccagctcctt gacccggtcg
     4141 cccagccagg gcgacaggcc gaggatcgat tcgtgcagcg cctcgatgcc gcgcgcccgc
     4201 acctgcgcgt ccgagccctt ggggatcagc agcgcgcatt gccagtagtc gttgcgcgcg
     4261 atcatcacca gcatgcggcc ggcgtcgacc tggccgaaca ggtcgggcgg atcgccgggc
     4321 acgcgcgaca cgcgcagcca caggacgtcc atcggggcgc ccagatccag cgtctcgagc
     4381 ccggcctggg cgcggatcgt cgaatggcgg ccgtcggccc cgaccaccag cgcggcgcgg
     4441 atctcgagct cgccctcggg cgtgcgcgcg cgcaggcccg cgacgcggcc gtcctcgcgg
     4501 atcagcgtcg tcgcctccgc cttcatcacc aggtggaacg acggcagccg gcgggcatgg
     4561 tcggccagga agtccaggaa gtcccactgc ggcatcatca ccaggaactt gcagtgggtg
     4621 ggcacgtggc ggaagtcggc gatctgcgcc tggtcggcgc cgacgcgcac ggcgacctcg
     4681 cgcagttcgt cgtggggccg gcgcaggaac tcgtcgagca ggtccagctc gtgcatcacg
     4741 tccagcgtcg acgggtggat ggtgtcgccg cggaagtcgc gcaggaagtc ggcgtgcttc
     4801 tccagcacca cgacatcgac gcccgcgcgg gcgagcagca agcccatcat catcccggcg
     4861 ggtccgccgc cggcgatgac gcaatccgtt ttcatgctgt ccacggaacc aatccctttc
     4921 cgccctctcc aacctggaac gcggcgtgcc gcgcaccggg agcaagggcc agtcccgagc
     4981 gggcacggtg ccgcgattga aggcgaatcg ccgttgttgc gcaagaccct cttcacattt
     5041 ggacaaattc acctgatatc gtcttcgaac cgatccatgg gcttggccgg gtaacgagtg
     5101 tgtgtcgacc ggtgcgtggt gcgccggtcg ttttcgggag cgctgggttc aactcagctt
     5161 cggacgcctt cgacggcgtc cgtttttttg tgggtccgcg gccccggcat acccgaaacg
     5221 agtgacgggc caccgtcgag tcgacaccta tgatcgacac atggccgctc gtcccaaact
     5281 cgtcgccgtc gtgtccgatc cgcctgtcga ccatgaccag gccgcgatcg ccgtgcttcg
     5341 ccagttccgc caggtcttca acgcggtcaa gtcgcacttc cagcaggtcg agaagacggt
     5401 gggcatgggc ggtgcgcagg tctgggcgct cagcgtggtg cgcgagcagc cgggtatcgg
     5461 cgtcggcgcg ctggcgcgcg cgatgtccat ccaccagtcc accgccagca acctggttcg
     5521 cacgctgatc gaccgcgaca tgctggtggc cgtcaagcaa ggcgccgacc gccgcgccgt
     5581 gcagctgaac ctgctgcccg ccggcgcccg ggtgctgcgc aatgccccgg gcccattcgc
     5641 cggcctgctg cccgacgcgc tcaaggcgct gccgccggag acgctggcgc ggctgcagtc
     5701 cgacctcgac caggtgatcg tcaggatcgc ggccgacgag gccgctgcgc acgtgccgct
     5761 ggccgggctc taggcatcgc cattgccgga accgctcgcc cccgagcgag gttcccatga
     5821 cgctgcccac cctcaccgcc acctccgact gcatcgccgc gtgcaccgcc tgcgccgcgg
     5881 cctgcgacca gtgcttcgcc ggctgcctca aggaggagga cgtcgccatg atggcgcgct
     5941 gcgtcgcgct ggatgtggac tgcgccgccg tgtgccggct ggcggcgggc gtgatgctgc
     6001 gcgacagccc gctcgccggg ccggtctgcg cgctgtgtgc gcaggtgtgc gacgcctgcg
     6061 cggacgagtg cgcgcagcac gaacacgagc attgccagtt gtgcgcccag gcgtgccggg
     6121 cctgcgccga atcgtgccgc gcgatggcga cgctcgcgcc ggattccgag cactgaacgc
     6181 ggatcggatg cgccgagcgg tcctcctgga cctggcgg
//
LOCUS       DATCRO010000421         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_994610, whole genome shotgun sequence.
ACCESSION   DATCRO010000421 DATCRO010000000
VERSION     DATCRO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994610"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VIN75_11420"
     CDS             complement(<1..513)
                     /locus_tag="VIN75_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974820.1"
                     /translation="MSDGARRAFAPTGRGLGAVLVVATLWVAWGLVRHPGFQPVSWLR
                     VAAPVVALLAGGGFTRLVAWARADADAQREFDRGGKALFMGFAMVAATLLAWLLVSQA
                     IPATWTALAGAPRAEAGVVTHRVPAAGDADCRFRLVVVSADAVAGAVPRPLDECVDES
                     VWTQAADGGPV"
     gene            complement(584..3355)
                     /gene="polA"
                     /locus_tag="VIN75_11425"
     CDS             complement(584..3355)
                     /gene="polA"
                     /locus_tag="VIN75_11425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8974821.1"
                     /translation="MTEKTLLLVDGSSYLYRAYHAMPDLRGPGGEPTGAIRGMVAMMK
                     LLREQIGADYAACVFDAKGKTFRDDWYPEYKANRSAMPEDLAKQVEPIHAVVAMLGWP
                     IITVPGIEADDAIGTLARAATSLGWKTVISTGDKDLAQLVDDHVTLINTMSNETLDVA
                     GVLAKFGVPPSQIIDYLSLIGDTVDNVPGVNKVGPKTAVKWLQEHGSLDGVIAAAPTM
                     KGAVGEHLRNALEWLPMGRKLVTVVTDHDLSGHVENWPALDALALRPVDHEKLLEFYQ
                     RYGFRGWREEIEKQLGTTPAPAPAVGADRGLFDDEVDVPAVPVQALAHQYDTIFTFEQ
                     LDEWIAKLQAAPLSALDTETDSLDAMRARIIGISFAVEPGKAAYIPLRHDYAGAPDQL
                     PFDDVIARLRPWLENPAAKKVGQHIKYDMHVFANIGIAVQGFEHDTLLQSYVLEAHRP
                     HGLSSLADRHLGRKGIDYEDLCGKGAKQIPFSQVDVERASRYSGEDSEMTLQVHQALY
                     PRIAAEPGMKFVYESIEMPTSAVLGRVERHGVLIDSARLAEQSRVLAERMMALENEAY
                     EIAGQPFNLGSPKQIGEILFNKLGLPVAKKTASGAPSTDESVLEKLALDYPLPAKLLE
                     HRGLSKLKGTYTDKLPQMINADTGRVHTHYAQAVAITGRLSSNDPNLQNIPIRTPEGR
                     RVREAFVAPPGHVIVSADYSQIELRLMAHISQDPGLLRAFNDGMDVHRATAAEVFNLA
                     SEEVTSEQRRYAKTINFGLIYGMGAFGLSQSLGIEQKAAKDYIDRYFARFAGVRRYME
                     ETKASASQKGYVETLFGRRISLPDINGGSGPLRAGAERQAINAPMQGTAADLIKLAML
                     AVQKALDDQGKGTRMVMQVHDELVFEVPEAEIEWARTEIPKLMAGVARLSVPLIAEVG
                     IGPNWDEAH"
BASE COUNT          466 a         1223 c         1211 g          545 t
ORIGIN      
        1 caccggcccg ccatcggcgg cctgcgtcca gaccgactcg tccacacact cgtccagcgg
       61 ccgcggcacc gcaccggcca cggcgtcggc gctgacgacg accaggcgga agcggcagtc
      121 ggcatcgccc gccgcgggca cgcggtgcgt cacgacgccg gcctcggcgc gcggcgcgcc
      181 ggccagggcc gtccacgtcg cggggatcgc ctgcgacacg agaagccagg ccagcaaggt
      241 cgccgccacc atcgcgaaac ccatgaacag cgccttgccg ccgcggtcga actcgcgctg
      301 cgcgtccgcg tccgcgcgcg cccaggccac caggcgcgtg aagccgccgc cggccagcag
      361 cgcgacgacc ggcgcggcga cgcgcagcca cgagaccggc tggaagcccg ggtgccttac
      421 gaggccccag gcgacccaca gcgtggcgac cacgagcacc gcgcccaacc ccctgcccgt
      481 cggcgcgaac gcgcgccgcg cgccgtcgct catggccgct gctccaggca ggcgcggcgc
      541 gcgcccgcac gcgtcacgcg acgcgcccgc gcgagcgcgg ccatcaatgc gcctcgtccc
      601 agttcggccc gatgcccacc tcggcgatca gcggcaccga cagccgggcg accccggcca
      661 tgagcttcgg gatctcggtc cgggcccatt cgatctcggc ctcgggcacc tcgaagacga
      721 gttcgtcgtg cacctgcatg accatgcgcg tgcccttgcc ctggtcgtcg agcgccttct
      781 gcaccgccag catcgccagc ttgatcaggt cggcggccgt gccctgcatc ggcgcgttga
      841 tggcctggcg ctcggcgccg gcgcgcagcg ggccgctgcc gccgttgatg tcgggcaggg
      901 agatgcggcg gccgaacagg gtctcgacgt agcccttctg cgacgcgctg gccttggttt
      961 cctccatgta gcgccgcacg cccgcgaagc gcgcgaaata gcggtcgatg tagtccttgg
     1021 ccgccttctg ctcgatgccc agcgactgcg acagcccgaa cgcgcccatg ccgtagatca
     1081 ggccgaagtt gatggtcttg gcgtagcggc gctgctcgct ggtgacctcc tcggacgcca
     1141 ggttgaacac ctcggcggcc gtggcgcggt gcacgtccat gccgtcgttg aacgcgcgca
     1201 gcaggcccgg atcctggctg atgtgcgcca tcaggcgcag ctcgatctgc gaatagtcgg
     1261 ccgacacgat cacgtggccc ggcggagcga cgaaggcctc gcgcacgcgc cggccctcgg
     1321 gcgtgcggat cgggatgttc tgcaggttcg ggtcgttgct ggacagccgt cccgtgatgg
     1381 ccaccgcctg cgcgtagtgc gtgtgcacgc ggccggtgtc ggcgttgatc atctgcggca
     1441 gcttgtccgt gtaggtgccc ttgagcttgg acagcccgcg gtgctccagc agcttggccg
     1501 gcagcggata gtcgagcgcc agcttctcca gcacgctctc gtcggtggac ggcgcgcccg
     1561 aggccgtctt cttggccacc ggcaggccca gcttgttgaa caggatctcg ccgatctgct
     1621 tgggcgagcc gagattgaac ggctggccgg cgatctcgta ggcctcgttc tcgagggcca
     1681 tcatgcgctc ggcgaggacg cgcgactgct ccgccaggcg ggcgctgtcg atcagcacgc
     1741 cgtggcgttc gacgcgtccc agcaccgcgc tggtgggcat ctcgatggac tcgtagacga
     1801 acttcatccc cggctcggcg gcgatgcgcg ggtacagcgc ctggtgcacc tgcagcgtca
     1861 tctcgctgtc ctcgccggag tagcgcgacg cgcgctcgac gtccacctgc gagaacggga
     1921 tctgcttggc gcccttgccg cacaggtcct cgtagtcgat gcccttgcgg cccaggtggc
     1981 ggtcggccag gctggacaac ccgtgcggcc gatgcgcctc cagcacgtag ctctggagca
     2041 gcgtgtcgtg ctcgaagccc tgcaccgcga tgccgatgtt ggcgaacacg tgcatgtcgt
     2101 acttgatgtg ctggcccacc ttcttggccg ccgggttctc cagccacggg cgcagccggg
     2161 cgatcacgtc gtcgaacggg agctggtcgg gcgcgccggc gtagtcgtgg cgcagcggga
     2221 tgtaggcggc cttgcccggc tcgacggcga acgagatgcc gatgatgcgg gcgcgcatgg
     2281 cgtcgagcga gtcggtctcg gtgtcgaggg ccgagagcgg cgcggcctgc agcttcgcga
     2341 tccattcgtc gagctgctcg aacgtgaaga tcgtgtcgta ctggtgcgcc agcgcctgca
     2401 cgggcacggc cggcacgtcg acctcgtcgt cgaacaggcc acggtcggcg ccaaccgccg
     2461 gcgcgggggc gggggtcgtg ccgagctgct tctcgatctc ctcgcgccag ccgcggaagc
     2521 cgtagcgctg gtagaactcc agcagcttct cgtggtcgac cgggcgcagc gccagcgcgt
     2581 cgagcgcggg ccagttctcc acgtgcccgc tcaggtcgtg gtcggtcacc accgtcacca
     2641 gcttgcggcc catcggcagc cactcgagcg cgttgcgcag gtgctcgccg acggcgccct
     2701 tcatcgtcgg cgcggcggcg atcacgccgt ccagcgagcc gtgctcctgc agccacttga
     2761 ccgccgtctt cgggccgacc ttgttgacgc cgggcacgtt gtcgacggtg tcgccgatca
     2821 gcgagaggta gtcgatgatc tgcgacggcg gcacgccgaa cttggccagg acgccggcga
     2881 cgtccagcgt ctcgttgctc atcgtgttga tcagcgtgac gtggtcgtcg accagttgtg
     2941 cgagatcctt gtcgccggtg gagatcaccg tcttccagcc cagcgaggtc gccgcgcgcg
     3001 cgagcgtgcc gatcgcgtcg tcggcctcga tgcccggcac ggtgatgatg ggccagccca
     3061 gcatcgcgac cacggcatgg atcggctcga cctgcttggc gaggtcttcc ggcatcgccg
     3121 agcggttggc cttgtactcg gggtaccagt cgtcgcggaa ggtcttgccc ttggcgtcga
     3181 acacgcaggc cgcgtagtcg gcgccgatct gctcgcgcag cagcttcatc atcgcgacca
     3241 tgccgcggat ggcgccggtg ggctcgccgc cggggccgcg caggtcgggc atcgcgtgat
     3301 aggcgcggta gaggtagctg gagccatcca ccaggagcag ggttttctcg gtcatggcgc
     3361 cattgtgccg ggtggggcgg gactcctcgt cgccacggct gccgcagccg gtgcgcccaa
     3421 cagagcttta cagccagcct tggga
//
LOCUS       DATCRO010000422         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_972219, whole genome shotgun sequence.
ACCESSION   DATCRO010000422 DATCRO010000000
VERSION     DATCRO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972219"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VIN75_11430"
     CDS             <1..1329
                     /locus_tag="VIN75_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8974822.1"
                     /translation="VQDLRMGGRQSNSTYQYTLKSDNQADLATWATKLAEAMKNEEAL
                     TDIDTDQQENGVEAFVTIDRDAAARQGMTNKDVDNALYDAFGQRQVATIYDALNQYHV
                     VMEASPRFTQTPLSLKDVQVPGSGANAAPVNGAASAAAAASTATVSANPGSRDASTGA
                     TVNTSAHTMVPLSTIASFSESAARASVNHQDGELATTVSYNLADGYSLSDAKAAIEHA
                     EASIHMPINVRGSSQGTAAAAQQNDNQQPLLILAAVVVIYIVLGVLYESLVHPLTVLS
                     TLPSAGVGAVLALMLFGMEFTLIALIGVFLLIGIVKKNAILIIDFALEAERSRGLSAH
                     DAVREACRLRFRPILMTTLAAALGALPLAIGFGEGSELRQPLGIAIIGGLVASQLLTL
                     LTTPVVYLLLDSWRRRSPDEKLLSRHTSDPDAHDPHPPLAPPAASAATGN"
     gene            1338..2855
                     /locus_tag="VIN75_11435"
     CDS             1338..2855
                     /locus_tag="VIN75_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8974823.1"
                     /translation="MGTPLPFKPAPAPRATPARPRVMRVALAAASLLALGACTALAPS
                     HKPEAPASPQFLEAPPGWMAAAPADTLSRGPWWTLFNDPVLDELAPQVAVSNQNVAAA
                     AAAVEESRAAVREQRASFFPTLSLDAGATRSGVGGKTSTSASSGSNPASTSASTSKTS
                     TRFTLGLGASWEPDLWGRIANTVSAASANAQASEADLANATLSAQAAFVTDYLSVREA
                     DAEIVTLQATIVGYQRALQITQNRYAAAIAQKSDVLQAQTTLANAQADLAQLRQQRAQ
                     LFHAMAVLAGQAPATFTLPAGDWNATTVPAIPVGVPSDLLQRRPDIASAERKVAAANA
                     DIGVARAAYFPSLTLSANGTQGASRLSDLFNASSFAWSLGVSLAETILDGGARTARVD
                     EAQAAWKQSVAQYRQTVLEAFSAVEDQLSAASSLEEQQALRRVASQAADQTEQEMQNR
                     YKQGLVAYTDVVTAQASALSARRALMQVSLNRQTAAINMVSALGGGWSTEKLAKD"
     gene            complement(3093..>3764)
                     /locus_tag="VIN75_11440"
     CDS             complement(3093..>3764)
                     /locus_tag="VIN75_11440"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY8974824.1"
                     /translation="RVQRALSQADVFRIDHFRGFAGYYEIPGDSPDAKRGEWKTGPGM
                     ALFDAIRKALGPLPIVAEDLGFITPDVVELRDACGFPGMRILQFGFGGDASNEFLPHN
                     YVRNTIAYTGTHDNDTARGWWDNASPHERAYVGSYLASGGDDIHWAMIRACCNSVANV
                     AVFQLQDVLGLGSAHRMNTPGTVGDQNWSWRFDWSMLGSEPGRVLGLISAASGRAPIG
                     LVHLP"
BASE COUNT          571 a         1386 c         1307 g          500 t
ORIGIN      
        1 gtgcaggacc tgcgcatggg cgggcgccag agcaactcga cctaccagta cacgctcaag
       61 tccgacaacc aggccgacct cgcgacctgg gcgacgaagc tcgccgaggc gatgaagaac
      121 gaggaggcgc tgaccgacat cgacaccgac cagcaggaga acggcgtcga ggccttcgtc
      181 accatcgacc gcgacgcggc ggcgcgacag ggcatgacca acaaggacgt cgacaacgcg
      241 ctgtacgacg cgttcggcca gcgccaggtc gccacgatct acgacgcgct gaaccagtac
      301 cacgtcgtga tggaggcgag ccctcggttc acgcagacgc cgctgtcgct caaggacgtg
      361 caggtgccgg gcagcggcgc caacgcagcg ccggtcaacg gcgcggccag cgccgcggcc
      421 gcggcgtcga cggccaccgt cagcgccaac ccgggctcgc gcgacgcctc caccggcgcc
      481 accgtcaaca ccagcgccca cacgatggtg ccgctgtcga cgatcgcgtc gttcagcgaa
      541 tcggcggcgc gcgccagcgt caaccaccag gacggcgagc tggcgacgac cgtctcgtac
      601 aacctggccg acggctactc gctgtccgac gccaaggccg cgatcgagca cgcggaggcg
      661 tccatccaca tgccgatcaa cgtgcgcggc agctcgcagg gcaccgcggc ggcggcgcag
      721 cagaacgaca accagcagcc gctgctgatc ctggcggccg tcgtcgtgat ctacatcgtg
      781 ctgggcgtgc tgtacgagag cctcgtgcac ccgttgacgg tgctgtcgac gctgccctcg
      841 gccggcgtcg gcgcggtgct ggcgctgatg ctgttcggca tggaattcac gctgatcgcg
      901 ctgatcgggg tgttcctgct gatcggcatc gtcaagaaga acgcgatcct gatcatcgac
      961 ttcgcgctgg aagccgagcg ctcgcgaggc ctgtccgctc acgacgcggt gcgcgaggcc
     1021 tgccgcctgc gcttccgccc gatcctgatg acgacgctgg ccgccgcgct cggcgcgctg
     1081 ccgctggcca tcggcttcgg cgagggctcg gagctgcgcc agccgctggg catcgccatc
     1141 atcggcgggc tggtcgccag ccagctgctg acgctgctga ccacgccggt ggtctacctg
     1201 ctgctggact cgtggcgccg ccgcagcccc gacgagaagc tgctgagccg gcacacctcc
     1261 gatccggacg cccacgaccc gcacccacct cttgcgcccc ccgccgccag cgcggccaca
     1321 gggaattgac gatgaccatg ggcactcccc tccccttcaa gccggcgccg gccccgcgcg
     1381 ccacgcctgc gcgaccgcgc gtcatgcgcg tcgcgctcgc ggccgcgagc ctgctcgcgc
     1441 tcggcgcctg cactgcgctc gccccgtcgc acaagcccga ggcgccggca tcgccgcagt
     1501 tcctcgaggc cccgccgggc tggatggccg ccgcgccggc cgacacgctg tcgcgcgggc
     1561 cctggtggac gctgttcaac gacccggtgc tggacgagct ggcgccacag gtggccgtct
     1621 ccaaccagaa cgtcgcggcc gcggcggccg ccgtcgagga atcgcgcgcc gccgtgcgcg
     1681 agcagcgcgc ctcgttcttc cccacgctgt cgctggacgc tggcgccacc cgatcgggcg
     1741 tgggcggcaa gacgagcacc tcggcctcgt cgggctccaa ccccgcctcg acgagcgcat
     1801 cgacctcgaa gaccagcacg cgcttcacgc tgggactggg cgcgagctgg gagcccgacc
     1861 tgtggggccg catcgccaac acggtgtccg cggccagcgc caacgcccag gcgagcgagg
     1921 ccgacctcgc caacgccacg ctgtcggcgc aggccgcgtt cgtcaccgac tacctctcgg
     1981 tgcgcgaggc cgacgccgag atcgtcacgt tgcaggccac catcgtgggc tatcagcgcg
     2041 cgctgcagat cacccagaac cgctacgccg ccgcgatcgc gcagaagagc gacgtgctgc
     2101 aggcgcagac gaccctggcc aacgcgcagg ccgatctcgc gcagctgcgc cagcaacgcg
     2161 cgcagctgtt ccacgcgatg gccgtgctcg cgggccaggc gccggccacg ttcaccctgc
     2221 ccgccggcga ctggaacgcc accaccgtcc cggccattcc cgtcggcgtg ccgtccgacc
     2281 tgctgcagcg ccggcccgac atcgcctcgg ccgaacgcaa ggtggcggcc gccaatgccg
     2341 acatcggcgt cgcgcgcgcc gcgtacttcc cgtcgctgac gctgagcgcc aacggcacgc
     2401 agggcgcgtc gcggctgtcc gacctgttca atgcgtcctc gttcgcgtgg tcgctgggcg
     2461 tgtcgctggc cgagacgatc ctggacggcg gcgcccgcac ggcacgcgtc gacgaggcgc
     2521 aggccgcgtg gaagcagtcg gtcgcgcagt atcgccagac cgtgctggag gcgttctccg
     2581 cggtcgagga ccagctgtcg gccgcatcgt cgctggagga gcagcaggcg ctgcgccgcg
     2641 tggcctcgca ggccgccgac cagaccgagc aggagatgca gaaccgctac aagcaaggcc
     2701 tggtcgccta caccgacgtg gtgaccgcgc aggcctcggc gctgagcgcg cgccgggcgt
     2761 tgatgcaggt gtcgctgaac cggcagacgg ccgccatcaa catggtgtcg gcgctgggcg
     2821 gcgggtggtc gacggagaag ctggcaaagg attgagcgcg cgcggcgtgc cgggctcgga
     2881 ggggtctggc ctctcctcat gtcgccgccg ggctgccgcc atcgcggggc aagcagccga
     2941 cgacatgagg cgaaggcctg acctcgatcc ccggccgatg atcggggtca ggccttcgcc
     3001 tcgaatcgcg accagcgtag aacctgcgat cgacgtggcc ggcgcgatcc gaggagaggc
     3061 cagacccctc cgaccgcggc cagcccgcct cgtcagggca gatgcaccag cccgatcggc
     3121 gcgcgtccgc tcgccgcgct gatcagaccc agcacgcggc cgggctcgct gcccagcatc
     3181 gaccagtcga agcgccacga ccagttctgg tcgccgacgg tgccgggcgt gttcatgcgg
     3241 tgggcgctgc ccaggcccag cacgtcctgc agctggaaca ccgccacgtt ggccaccgag
     3301 ttgcagcagg cgcggatcat cgcccagtgg atgtcgtcgc cgccgctggc caggtagctg
     3361 ccgacgtagg cgcgctcgtg cgggctcgcg ttgtcccacc agccgcgcgc ggtgtcgttg
     3421 tcgtgcgtgc cggtgtaggc gatcgtgttg cgcacgtagt tgtgcggcag gaattcgttg
     3481 gacgcgtcgc cgccgaagcc gaactgcagg atgcgcatgc cggggaagcc gcaggcgtcg
     3541 cgcagctcga cgacatcggg cgtgatgaag ccgaggtcct cggcgacgat gggcagcggg
     3601 ccgagcgcct tcctgatggc gtcgaacaag gccatgccgg gaccggtctt ccattcgccg
     3661 cgcttggcgt cggggctgtc gcccgggatc tcgtagtagc cggcgaagcc gcggaaatgg
     3721 tcaatgcgaa agacgtccgc ctggctcagg gcgcgctgca cgcg
//
LOCUS       DATCRO010000423         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_375680, whole genome shotgun sequence.
ACCESSION   DATCRO010000423 DATCRO010000000
VERSION     DATCRO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375680"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VIN75_11445"
     CDS             complement(<1..263)
                     /locus_tag="VIN75_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8974825.1"
                     /translation="MQRSAGAIDRFLDGLWLEDGLAKNSLAAYRRDLTLFAAWLGHEQ
                     SRDLAQARPSDVQGYMLARHAASKATSANRRLAVFRRFYRWGLR"
     gene            complement(331..993)
                     /locus_tag="VIN75_11450"
     CDS             complement(331..993)
                     /locus_tag="VIN75_11450"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8974826.1"
                     /translation="MIRPSWRHPARTAASGARALGQGLLGMALIALAAFALLPSANAA
                     TGPVAITMENQTFPAQASVAGAPLQLNGVGLRAAFIYKVYLAGLYLPAKAATGADAMA
                     QPGPKRVQVRMLVDGPSDEFAKAFTGGIAKRTPADQVAAMKDRIAAFDHTLRSVGKVH
                     KGDVVDLDWLPSEGLAMRINGKPVGTPVPGADLYAAMLNIFVGDRPVDAKMKAGMLGA
                     AS"
     gene            complement(996..2150)
                     /gene="queG"
                     /locus_tag="VIN75_11455"
     CDS             complement(996..2150)
                     /gene="queG"
                     /locus_tag="VIN75_11455"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY8974827.1"
                     /translation="MLQLRHEVGQDPAKVALLRRLEGWARELGFSQIGVAEVDLGDAE
                     QGLRAWLEAGFHGSMGYMAAHGMKRARPAELQPGALRIITARMDYLPQARGEQWQAQE
                     TARLARPGEAVISMYARGRDYHKVLRNRLQKLADRLAAEIGPLGHRCFTDSAPVLEVE
                     LATRSGLGWRGKHTLALARDAGSMFFLGEIFVDLALPLTAPATPHCGQCSACIDVCPT
                     QAIVAPERLDARRCISYLTIESDDPIPIEFRRAIGNRIYGCDDCQVACPWNKFARRTS
                     LPDFAERPDLAEPSLLELFAWSEAEFLRRTEGSPIRRIGHARWQRNLAVALGNALHAG
                     EPRAPIEAALRGRLADASPLLAEHIAWALSSAPGAGALNERGAASGAAKE"
     gene            2133..2639
                     /gene="tsaE"
                     /locus_tag="VIN75_11460"
     CDS             2133..2639
                     /gene="tsaE"
                     /locus_tag="VIN75_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8974828.1"
                     /translation="MTQLQHPPILGTSSADWPDEAACARDAAALARLFRDDLALRSAF
                     IELEGPLGAGKTTFVRHLLHALGVEGRIKSPTYAVVETYELEHLAISHFDFYRFDDPQ
                     EWEDAGLRDLFGAPGLKLVEWAEKAEPLLPVPDLRVTITPHDEAIRRVRYDAMTPTGL
                     LLIENTGS"
     gene            2636..4054
                     /locus_tag="VIN75_11465"
     CDS             2636..4054
                     /locus_tag="VIN75_11465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8974829.1"
                     /translation="MKRRDFVRGGSLVLCLGATDLAFGASIVAVRIWPAADYTRVTIE
                     SDEPLQARNFLADNPNRLVVDIENLELSPALRDMVGKVRADDPFIAGVRVGQNTPRVV
                     RLVLDLKQAVAPQQFMLAPVAAYQHRLVFDLYPVREQDPLLALLRGKEQSEQRAAQEV
                     QDALGEFIGRIPAGASAPVVPGTNIASLPPPPGSSGPRVAEVGSAIHGGLGVDTSTME
                     TQAPFAARPLTPEEKRKVDRLLVIAIDPGHGGEDPGATGPTGLHEKDVVLAIALQLRE
                     RLNAKPGVRVLLTRDSDFFVPLGERVKKAERVQADLFVSIHADAFFTPDAKGASVFAL
                     SQGGASSAAARWMANKENHSDAVGGVSVKVKDAQVMRAMLDMSTTAQIKDSLRVGGEV
                     LLRMGKVGKLHRGQVEQASFAVLKAPSIPSILVETAFISNPQEEDKLRDPDYRTKLVE
                     ALYTGIDRYFAKNPPIARHRDL"
     gene            4196..4669
                     /locus_tag="VIN75_11470"
     CDS             4196..4669
                     /locus_tag="VIN75_11470"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY8974830.1"
                     /translation="MSLPLKHVLVAAAIAAAGSGSAFAQQRDERGAPHGHVMHPAEPH
                     AGPNGYHRPEAPQGWDARPKTFDRAAYQHNFQAARSYHVGPYQRPAGWVNRHWSYGER
                     LPRAYWAPQYLLTDYWLFGLEVPPVDYEWVRVGADALLIDVHTGEILQAEYGVFG"
     gene            complement(5106..>5511)
                     /locus_tag="VIN75_11475"
     CDS             complement(5106..>5511)
                     /locus_tag="VIN75_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8974831.1"
                     /translation="VIGNQTNYTIGRWIGPRVFHWENSRWFNKEGFDKSHAFFEKYGG
                     AALIAGRFMPFVRTFAPFVAGVTCMTRRRFTIYDVTGAALWIGSVTLLGYGIGNLPWV
                     KGHLQWIFLSMIIIPGVLALVGILRKKAEPAR"
BASE COUNT          815 a         1982 c         1932 g          782 t
ORIGIN      
        1 cgcaaccccc agcggtagaa gcgccggaac accgccagcc gccggttcgc gctggtggcc
       61 ttgctcgcgg cgtggcgcgc cagcatgtag ccctgcacgt cgctcgggcg cgcctgcgcg
      121 aggtcgcgcg actgctcatg gcccagccac gccgcgaaca gcgtgaggtc gcgccggtag
      181 gccgcgagcg agttcttcgc cagcccgtct tccagccaca ggccgtcgag gaagcggtcg
      241 atcgcgccgg cgctgcgctg catcgcctcg ctcgtgggcg ccgcggccgg gcgcgcgcgc
      301 gggggggagg ccttcttcgc cgtggccaag tcagctcgcc gcgcccagca tgccggcctt
      361 catcttcgcg tcgaccggcc ggtcgccgac gaagatgttc agcatcgcgg cgtagaggtc
      421 ggcgcccggc accggcgtgc ccaccggctt gccgttgatg cgcatcgcca ggccctcgga
      481 cggcagccag tcgaggtcga cgacgtcgcc cttgtgcacc ttgcccacgc tgcgcagcgt
      541 gtggtcgaac gcggcgatgc gatccttcat cgcggccacc tggtcggccg gcgtgcgctt
      601 ggcgatgccg ccggtgaagg ccttggcgaa ctcgtcggac gggccgtcca ccagcatgcg
      661 cacctgcacg cgcttgggcc cgggctgggc catcgcgtcc gcgccggtgg cggccttggc
      721 gggcaggtac aggcccgcga ggtagacctt gtagatgaac gcggcgcgca ggcccacgcc
      781 gttgagctgc agcggcgcgc cggcgacgct cgcctgggcc gggaacgtct ggttctccat
      841 cgtgatcgcg acgggcccgg tcgcggcgtt ggcgctgggc agcagcgcga aggcggccag
      901 cgcgatcagc gccatgccca gcaggccctg ccccagcgcg cgcgcgccgg aggccgcggt
      961 gcgggcggga tggcgccatg acggtcggat catcttcatt ccttggcggc gcccgatgcg
     1021 gcgcctcgct cgttcaacgc gccagcgccg ggcgcggacg acagggccca cgcgatgtgt
     1081 tcggccagca gcgggctcgc gtcggcgagg cgcccgcgca gcgcggcctc gatgggcgcg
     1141 cgcggctcgc cggcgtgcag cgcgttgccc agggccacgg cgaggttgcg ctgccagcgc
     1201 gcgtggccga tgcggcggat cggcgagcct tcggtgcggc gcaggaattc cgcctcgctc
     1261 caggcgaaca actccaggag cgacggctcg gccaggtcgg ggcgctctgc aaagtcaggt
     1321 aaagacgtgc ggcgcgcgaa cttgttccac gggcaggcca cctggcagtc gtcgcagccg
     1381 tagatgcggt tgccgatcgc gcggcggaac tcgatcggga tcgggtcgtc gctctcgatc
     1441 gtcaggtagg agatgcagcg gcgcgcgtcc aggcgctcgg gcgcgacgat cgcctgcgtg
     1501 gggcagacgt cgatgcaggc gctgcactgc ccgcaatgcg gcgtcgcagg cgcggtgagc
     1561 ggcagcgcga ggtcgacgaa gatctcgccg aggaagaaca tcgagccggc gtcgcgcgcg
     1621 agcgccagcg tgtgcttgcc gcgccagccc aggcccgaac gggtggccag ctccacctcg
     1681 agcaccgggg cggagtcggt gaagcagcgg tggcccagcg ggccgatctc ggcggcgagg
     1741 cggtcggcca gcttctgcag ccggttgcgc agcaccttgt ggtagtcgcg gccgcgggcg
     1801 tacatcgaga tgacggcctc gccggggcgc gcgaggcggg cggtctcctg cgcctgccac
     1861 tgctcgccgc gcgcctgagg caggtagtcc atgcgcgccg tgatgatgcg cagcgcgccg
     1921 ggctgcagct cggccgggcg cgcgcgcttc atgccgtgcg ccgccatgta gcccatgctg
     1981 ccgtggaagc cggcctcgag ccacgcgcgc aggccctgct cggcatcgcc gaggtcgacc
     2041 tccgcgacgc cgatctgtga gaatcccaac tcgcgcgccc agccctcgag gcgccgcaag
     2101 agagcgacct tcgcgggatc ctgcccgacc tcatgacgca gctgcaacat ccgccaattc
     2161 taggaacatc gagcgccgac tggcccgacg aagcggcttg cgcgcgcgac gcggccgcgc
     2221 tcgcgcggct ttttcgtgac gatctggcgc tgcgcagcgc gttcatcgag ctggagggcc
     2281 cgctgggcgc cggcaagacg accttcgtgc gccacctgct gcatgcgctg ggcgtcgagg
     2341 gccgcatcaa gagccccacg tacgcggtgg tcgagaccta cgagctcgag cacctggcca
     2401 tctcgcactt cgacttctac cgcttcgacg acccgcagga atgggaggac gccggcctgc
     2461 gcgacctgtt cggcgcgccc ggcctcaagc tggtggaatg ggccgagaag gccgagccgc
     2521 tgctgcccgt gcccgacctg cgcgtgacca tcacgccaca cgacgaggcg attcgacggg
     2581 tccgatacga cgcgatgaca cccacggggc tgcttctgat cgagaatacg ggttcatgaa
     2641 gcgtagagac ttcgtgcgcg gcggcagcct cgtgctgtgc ctgggcgcca ccgacctggc
     2701 gttcggcgcc agcatcgtcg ccgtccgcat ctggcccgcc gccgactaca cgcgcgtcac
     2761 catcgagtcc gacgagccgc tgcaggcgcg caacttcctg gccgacaacc cgaaccgcct
     2821 ggtcgtcgac atcgagaacc tggagctcag tcccgcgctg cgcgacatgg tgggcaaggt
     2881 gcgcgcggac gacccgttca tcgcgggcgt gcgcgtcggc cagaacacgc cgcgcgtcgt
     2941 gcgcctggtg ctcgacctga agcaggccgt cgcgccgcag cagttcatgc tcgcgccggt
     3001 ggccgcctac cagcaccgcc tggtgttcga cctctatccg gtacgcgagc aggatccgct
     3061 gctcgcgctg ttgcgcggca aggagcagtc cgagcagcgc gccgcgcagg aggtgcagga
     3121 cgcgctcggc gagttcatcg gccgcattcc cgccggcgcc agcgcgccgg tggtgccggg
     3181 caccaacatc gcgtcgctgc cgccgccccc gggcagctcg ggcccgcgcg tggcggaggt
     3241 cggctccgcg atccacggcg gcctgggcgt cgacacgtcc acgatggaga cgcaggcgcc
     3301 gttcgcggcg cgtccgctca ccccggagga aaagcgcaag gtcgaccgcc tgctcgtgat
     3361 cgccatcgac ccgggccacg gcggcgagga tccgggcgcc accggcccca ccgggctgca
     3421 cgagaaggac gtcgtgctgg ccatcgcgct gcagctgcgc gagcgcctga atgccaagcc
     3481 cggggtgcgc gtgctgctca cgcgcgactc cgacttcttc gtgccgctgg gcgagcgcgt
     3541 gaagaaggcc gagcgcgtgc aggccgacct gttcgtctcc atccacgccg acgcgttctt
     3601 cacgcccgac gccaagggtg cctcggtgtt cgcgctgagc cagggcggcg cctcgagtgc
     3661 ggccgcccgc tggatggcca acaaggagaa ccattccgac gcggtcggcg gcgtcagcgt
     3721 caaggtcaag gacgcgcagg tcatgcgcgc catgctggac atgagcacca cggcgcagat
     3781 caaggacagc ctgcgcgtcg gcggcgaggt gctgttgcgc atgggcaagg tcggcaagct
     3841 gcaccgcggc caggtggaac aggccagctt cgcggtgctc aaggcgccgt ccatcccgtc
     3901 gatcctcgtc gagacggcgt tcatctcgaa cccgcaggaa gaggacaagc tgcgcgaccc
     3961 cgactaccgc accaagctgg tcgaggcgct gtacacgggg atcgaccgct acttcgcgaa
     4021 gaatccgccg atcgcccggc atcgcgacct gtaggacggc gccgcgacct gcgccctggc
     4081 cgccccgccg ggcgtcttcg tgtcggtccc gctgaacttc ttcacatccg cgagagctgc
     4141 cgagcctacc ttgcgaagca acggacaaac gtcgtccgtc tccaaggagt tcccgatgtc
     4201 acttcccctg aagcacgtgc tcgtcgcggc cgccatcgcc gcggcaggca gcggatcggc
     4261 cttcgcgcag cagcgcgacg aacgcggcgc gccgcacggg cacgtcatgc atcccgccga
     4321 gccccatgcc ggcccgaacg gctaccaccg ccccgaggcc ccgcaaggct gggacgcgcg
     4381 ccccaagacc ttcgaccggg cggcgtacca gcacaatttc caggccgccc gcagctacca
     4441 cgtcggcccg taccagcgcc ccgcgggctg ggtcaaccgc cactggagct acggcgagcg
     4501 cctgccgcgc gcctactggg cgccgcagta cctgctcacc gactactggc tgttcggcct
     4561 cgaggtgccg ccggtcgact acgaatgggt gcgcgtgggc gccgacgcgc tgctgatcga
     4621 cgtgcacacc ggcgagatcc tgcaggccga gtacggcgtg ttcggctgag gccgggccgg
     4681 cgtcggatct tgcgtcctgg cgcgcctggc cgcttaccct ggggtcaggt cttgaatgtt
     4741 gcgcgctcat ccgcttctcg atggggtcag gtcttgaaag ttgcgcggca cggacggtac
     4801 cagtcgcgac tgtcccctcg cgcaactttc aagacctcac cccagatgga ggccgtgccg
     4861 cgcaacattc aagacctgac cccgctggcg cggctgtccc ctcgcgcaac tttcaagacc
     4921 tgaccccaga ctgaggccgt gccgcgcaac attcaagacc tgaccccgct ggcgcggctg
     4981 tcccctcgct cagctttcaa gacctgaccc cagacggagg ccgtgccgcg caacattcaa
     5041 gacctgaccc cgctggcgat gccgtggcgc gcaaaaggca tgccccgaca ccgcggagcc
     5101 gcgcctcaac gcgccggctc cgccttcttg cgcaggatcc cgaccagcgc cagcacgccc
     5161 gggatgatga tcatcgacag gaagatccac tgcaggtggc ccttgaccca cggcaggttg
     5221 ccgatgccgt agcccagcag cgtcacgctg ccgatccaca gggcggcgcc ggtgacgtcg
     5281 tagatcgtga aacggcggcg ggtcatgcag gtgacgccgg ccacgaacgg ggcgaaggtg
     5341 cgcacgaagg gcatgaagcg gccggcgatg agcgcggcgc cgccgtactt ctcgaagaac
     5401 gcgtgcgact tgtcgaagcc ttccttgttg aaccagcgcg agttctccca gtggaagacg
     5461 cgcggcccga tccagcggcc gatcgtgtag ttggtctggt tgccgatcac c
//
LOCUS       DATCRO010000424         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_713369, whole genome shotgun sequence.
ACCESSION   DATCRO010000424 DATCRO010000000
VERSION     DATCRO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713369"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..536)
                     /gene="gspG"
                     /locus_tag="VIN75_11480"
     CDS             complement(117..536)
                     /gene="gspG"
                     /locus_tag="VIN75_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8974832.1"
                     /translation="MPARRADSAGFTLLELLVVIVIIGLLAGLVAPRYFDQVSKSNTK
                     IAKAQIDSLEKALDEYRLDVGVYPTTEQGLEALNTRPQNVDKWAGPYLKKAVPLDPWG
                     ASYVYKSPGEHGEYDLLSYGKDGQPGGSGEASDVTSW"
     gene            890..1585
                     /locus_tag="VIN75_11485"
     CDS             890..1585
                     /locus_tag="VIN75_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8974833.1"
                     /translation="MLLYVLDVTPCTKVTQEGSMIRLLLADDHRMFREGLSRLLSDQA
                     DLEVIAEAASAAEALDAVRRHEIDVAVFDLSMPGRSGPELISHSKSLRPDLRVLVLTM
                     HAEEPHVTQSLRAGADGYATKELAAAEMIAAIRRLRAGGRYLCPEVAERLALGIATNQ
                     HGDQRHARLSDREYRVFEMLVAGKRGWEIASELSLSEKTVSTHKINVLRKMNVANRTE
                     LIRYAIREQLVAA"
     gene            1818..2285
                     /locus_tag="VIN75_11490"
     CDS             1818..2285
                     /locus_tag="VIN75_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974834.1"
                     /translation="MSPYRRSGDQAAASAVSPEAAPQPRGGRRARKRVDLTSDSPAAR
                     VQRMLVLVERFRALSVLRSDPVRLVDLLRGIISIAREAFGTELSRDWTARTVVQMAPD
                     QQRCLRDLERLLQGLAYGSPDAWRGLGHAMDALLIQCLLLQNPGDGPVEADDR"
     gene            complement(2304..>2613)
                     /locus_tag="VIN75_11495"
     CDS             complement(2304..>2613)
                     /locus_tag="VIN75_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974835.1"
                     /translation="RYGAPRARATELMLLRIAQEALWNSARHAAARHVRISFDCLGSR
                     SRFTIRDDGCGFDPAALGATGRMPGLGILNMRERALSADALFSLDSAPGRGTLIAVDF
                     "
BASE COUNT          391 a          894 c          910 g          418 t
ORIGIN      
        1 cctgcaagcg ggcggccgcc gcgtcggcgg cgtccagcgt cagcgtgcac acgccggtcg
       61 cgccgcgcac cacgagttcg aagcgggagg ccatggtcgt cttcgccttt cgcgcatcac
      121 caggacgtga cgtcggacgc ctcgcccgag ccgccgggct ggccgtcctt gccgtacgag
      181 agcaggtcgt attcgccgtg ctcgcccggc gacttgtaga catagctcgc gccccacggg
      241 tcgagcggca ccgccttctt gagatagggg ccggcccact tgtcgacgtt ctgcggacgc
      301 gtgttcaagg cctcgagtcc ctgctcggtg gtcggataga cgccgacatc gaggcgatat
      361 tcgtcgagcg ccttctcgag cgagtcgatc tgcgccttgg cgatcttcgt gttggacttg
      421 ctgacctggt cgaagtagcg cggcgccacg aggcccgcga gcaggccgat gatcacgatc
      481 acgaccagca gctcgagcag cgtgaagccc gcggaatccg ctcggcgggc aggcatcgag
      541 gcctggcgcg gcgcgatgcg cgcgaatgcg cacgacgatc gccgtgacat cccgcgcggc
      601 ccgctggggt aggtgtgcgg cagcaatcca ggtccctcct gtgtgccttc ttctgttgag
      661 caaagtctgc ggatctgctc ttaaggactg cttaagtcgg aggggtgaca cccccacttt
      721 gtggtgcgga caactaccaa cccgttgtcg gcaccgcacc cgacccgcca tgacgcgagg
      781 gaccttctcc ataggtgcgc ggcgatcgcg acctccccga atttggggag tcctggggtt
      841 gcatccccat tgcggatagg acgctgccta ctttcgcttt gctcgacgat tgctactcta
      901 tgttctagat gtgacgccat gtaccaaagt cacgcaggag ggaagcatga ttcgtttgct
      961 gttggccgac gaccatcgga tgtttcgcga gggcctgagt cgcttgttgt ccgaccaggc
     1021 ggacctggag gtgatcgccg aggcggccag cgccgccgag gcgctcgacg ccgtgcggcg
     1081 ccacgagatc gatgtcgcgg tgttcgacct gtcgatgcct ggccgcagcg gccccgagct
     1141 gatcagccac tcgaagtcgc tgcgacccga tctgcgcgtg ctcgtgctca cgatgcatgc
     1201 ggaggagcct catgtgacgc agtcgctgcg cgctggcgcc gacggctatg ccaccaagga
     1261 actcgcggcg gcggagatga tcgcggcgat ccggcgcctg cgcgcgggcg gccggtacct
     1321 gtgcccggag gtcgcggaac gactcgctct gggcatcgcg acgaaccagc atggcgacca
     1381 gcgccatgcg cggctgtccg atcgagagta ccgggtcttc gagatgctgg tcgcgggcaa
     1441 gcgcggatgg gagatcgcgt cggagctgtc gctcagcgag aagacggtga gcacgcacaa
     1501 gatcaacgtg ctgcgcaaga tgaacgtggc caaccgcacc gagctgatcc gctatgcaat
     1561 ccgcgagcag ctcgtggcgg catagcccgg cgacgcggcg ggcgaaccgg cccggccgct
     1621 gccgcgccgc tgcgccccgt cctgccgcgc cgcattggcg acggctggcg cagcgcgggc
     1681 cggaccacta ccatgcagac gaagcaagtg gcccgtgcgg tgccgagccc gcacctgccc
     1741 gcggtcgcgt gaccggcgac gtcgaccgga tccgcgccgc agcagcagtc gtccttccat
     1801 cgacggagct tctcgacatg tcgccatacc gccgctcagg ggaccaggcc gccgcgtccg
     1861 cggtgtcgcc cgaggccgcc ccgcagcctc gcggcggccg gcgcgcgcgc aagcgcgtcg
     1921 acctgacatc cgattcgccg gcggccaggg ttcagcgcat gctggtgctg gtcgaacgtt
     1981 tccgggcgct gtccgtcctg cgttccgatc ccgtgcggct cgtcgacctg ctgcgcggca
     2041 tcatctcgat cgcgcgcgag gccttcggca ccgagctgtc gcgcgactgg acggcgcgca
     2101 ccgtggtgca gatggcgccc gaccagcagc gctgtctgcg agatctcgaa cgtctcctgc
     2161 agggactggc ctacggctcg ccggacgcct ggcgcggcct gggccacgcg atggacgcgc
     2221 tgctgatcca gtgcctgctg ctccagaatc cgggtgacgg acccgtcgag gccgacgatc
     2281 gctgacgcgg gcgccgtcgg acttcagaag tcgacggcga tgagcgtgcc gcggcccggc
     2341 gctgaatcca gcgagaacag cgcgtccgcg gacaaggcgc gctcgcgcat gttcaggatg
     2401 cccagccccg gcatgcggcc ggtggctccc agtgcggcgg gatcgaaccc gcagccgtcg
     2461 tcgcggatcg tgaaccggct gcggctgccg aggcagtcga acgaaatgcg cacgtggcgg
     2521 gccgcggcat gccgggcgct gttccacagg gcttcctgcg cgatgcgcag cagcatcagt
     2581 tcggtcgccc gcgcccgcgg cgcgccgtag cgc
//
LOCUS       DATCRO010000425         3928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_105714, whole genome shotgun sequence.
ACCESSION   DATCRO010000425 DATCRO010000000
VERSION     DATCRO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105714"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VIN75_11500"
     CDS             <1..364
                     /locus_tag="VIN75_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974836.1"
                     /translation="RLHRVAYAMDQWCAIVAALGFARQWNPADSRARRYLTEAIFPFY
                     IVHQTAIVLLAHFMKPLGLRPPVEGPLLVAATAAICLASFEIVRRVGWMRPLFGLAPA
                     PAASSRPASLGTAPARAG"
     gene            complement(381..890)
                     /locus_tag="VIN75_11505"
     CDS             complement(381..890)
                     /locus_tag="VIN75_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974837.1"
                     /translation="MVQRVQVVALAISVAIGAAVVTGLTLGSREAPVHVGPTTLPALD
                     LVDVYATILVNLAGPASSPSPRTISVSRTIYDTCVPGSPKNLPAPCGRTKVGKLKPDI
                     EAALHEALAAQGFDARFGDEGDLSLHQVVTEATGTPAADGDVGHSNRMKFHFKRVGGR
                     LQLQGTSPE"
     gene            complement(913..2496)
                     /gene="purH"
                     /locus_tag="VIN75_11510"
     CDS             complement(913..2496)
                     /gene="purH"
                     /locus_tag="VIN75_11510"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8974838.1"
                     /translation="MSLTALLSVSDKTGIVDFARALHDSGVKLLSTGGTAKLLAESGL
                     PVTEVSEITGFPEMLDGRVKTLHPRVHGGLLARRDVPEHMAALKEHGIGTIDLLIINL
                     YPFAKATANPDCTLEDAIENIDIGGPAMLRAAAKNWQDVAVVIDPADYPQVLGELKGA
                     GLQRKTKFALAKKVFAHTAAYDGMITYYLTALGEDAELKGEAVPARGDYPATFTLQLT
                     KVQDMRYGENPHQSAAFYRDAQPAPGTLASWKQLQGKELSFNNIADSDAAWECVKTFD
                     VPACVIVKHANPCGVAIGASVAEAYAKAFKTDPTSAFGGIIAFNRALDGAAAEAVARQ
                     FVEVVIAPSVTPEARAVFAAKQNVRVLEVALPEGSKAMNALDFKRVGGGVLLQSTDAR
                     NVAQSELKVVTKVQPTAEQLVDLMFAWKVAKFVKSNAIVFCAGGMTMGVGAGQMSRID
                     SARIASIKAENAGLTLKGTAVASDAFFPFRDGLDVVCDAGATCVIQPGGSMRDEEVIN
                     AADERGIAMVLTGTRHFRH"
     gene            complement(2561..2800)
                     /locus_tag="VIN75_11515"
     CDS             complement(2561..2800)
                     /locus_tag="VIN75_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8974839.1"
                     /translation="MSKKQIEACVRETLEQYFKDLHGVEPHSMHDMFMNTVEKPLLDV
                     VLRHADGNQSKAAEWLGINRNTLRRKLLEHKLVDH"
     gene            complement(2851..3879)
                     /gene="dusB"
                     /locus_tag="VIN75_11520"
     CDS             complement(2851..3879)
                     /gene="dusB"
                     /locus_tag="VIN75_11520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY8974840.1"
                     /translation="MHIGAIELPNALFVAPMAGVTDRPFRMLCKRLGAGYAVSEMVTS
                     RKDLWHTLKTSRRANHDGETAPISVQIAGTDAQMMADAARYNIDRGARIIDINMGCPA
                     KKVCNKWAGSALMQDEPLALEIVEAVVAACAPANVPVTLKMRTGWCDAERNAVRIARA
                     AESAGVAMVTVHGRTREQGYKGFAEYDTIAAVKAALRIPVVANGDIDSPHKALQVLQA
                     TGADALMIGRAAQGRPWIFREVAHFLSTGELLAPPATRDVARWLTEHLHDHYGLYGED
                     LGVRTARKHIGWAVHALPGGPEFRAVMNTHADCKTQMAGVIDYFDQLADRHTLWPAAN
                     EDLLRQEA"
BASE COUNT          509 a         1334 c         1410 g          675 t
ORIGIN      
        1 ccggctgcat cgcgtcgcct acgcgatgga ccagtggtgc gcgatcgtcg ccgcgctcgg
       61 cttcgcccgc cagtggaacc cggctgactc gcgcgcgcgg cgctacctga ccgaggcgat
      121 cttcccgttc tacatcgtgc atcagaccgc gatcgtgctg ctggcgcact tcatgaagcc
      181 gctgggcctg cgcccgccgg tcgagggccc gctgctggtc gcggcgacgg cggcgatctg
      241 cttggcgagc ttcgagatcg tgcgccgcgt cggctggatg cgtccgctgt tcggcctggc
      301 acccgcgccg gcggcgtcgt cgcggccggc atcgctcggc acggcaccgg ctcgagccgg
      361 atgacgcgca ggtcggtgac ctattccggc gacgtgccct gcagctgcaa tcgtccgccg
      421 acgcgcttga aatggaattt catgcggttc gagtgcccga cgtcgccgtc cgcggccgga
      481 gtgcccgtcg cctcggtgac gacctggtgc aacgacaagt cgccctcgtc gccgaaccgc
      541 gcgtcgaacc cctgcgcggc cagcgcctcg tgcagggccg cctcgatgtc gggcttgagc
      601 ttgccgacct tggtccggcc gcacggcgcg ggcaggttct tcggcgaccc tggcacgcag
      661 gtgtcgtaga tcgtccgcga caccgagatc gtgcgcggtg acggcgacga ggcgggcccg
      721 gccaggttca cgaggatggt cgcgtagacg tcgaccaggt cgagcgccgg cagcgtcgtc
      781 ggcccgacgt gcacgggcgc ctcgcgcgac ccgagggtca gcccggtcac caccgccgcg
      841 ccgatcgcca ccgaaatggc cagcgcgacc acctggaccc gttgaaccat gccgtcgcgt
      901 ccgcccggcg gctcagtggc ggaagtggcg ggtgccggtc agcaccatcg cgatgccgcg
      961 ctcgtcggcc gcgttgatca cttcctcgtc gcgcatcgag ccgcccggct ggatgacgca
     1021 ggtggcgccg gcgtcgcaga cgacgtccag gccgtcgcgg aacgggaaga aggcgtcgct
     1081 ggcgacggcc gtgcccttga gcgtcaggcc ggcgttctcc gccttgatgc tggcgatgcg
     1141 cgcggagtcg atgcggctca tctggcccgc gcccacgccc atcgtcatgc cgcccgcgca
     1201 gaacacgatg gcgttgctct tgacgaactt ggccaccttc cacgcgaaca tcaggtcgac
     1261 cagctgttcg gcggtcggct gcaccttggt cacgaccttg agctcggact gcgccacgtt
     1321 gcgcgcgtcc gtggactgca gcagcacgcc gccgcccacg cgcttgaagt cgagcgcgtt
     1381 catggccttg gagccttcgg gcagcgccac ttccagcacg cgcacgttct gcttggcggc
     1441 gaagacggcg cgcgcctccg gcgtcacgga cggcgcgatc accacttcga cgaactggcg
     1501 tgcgacggcc tcggccgcgg cgccgtccag ggcgcggttg aaggcgatga tgccgccgaa
     1561 ggccgacgtc gggtcggtct tgaacgcctt ggcgtaggcc tcggcgacgc tggcgccgat
     1621 cgccacgccg cacgggttgg cgtgcttgac gatcacgcag gccggcacgt cgaaggtctt
     1681 gacgcattcc caggccgcgt cggagtcggc gatgttgttg aacgacagtt ccttgccctg
     1741 cagctgcttc cagctcgcga gcgtgcccgg cgccggctgc gcgtcgcggt agaacgcggc
     1801 gctctggtgc gggttctcgc cgtagcgcat gtcctgcacc ttggtcagct gcagcgtgaa
     1861 cgtggcggga tagtcgccgc gcgcgggcac ggcctcgccc ttgagctcgg cgtcctcgcc
     1921 cagcgcggtg aggtagtagg tgatcatgcc gtcgtaggcg gccgtgtgcg cgaacacctt
     1981 cttggcgagc gcgaacttgg tcttcctctg caggccggcg cccttgagct cgcccagcac
     2041 ctgcgggtag tcggccgggt cgatcacgac ggcgacgtcc tgccagttct tggccgcggc
     2101 gcgcagcatc gccgggccgc cgatgtcgat gttctcgatg gcgtcttcca gcgtgcagtc
     2161 cgggttggcc gtggccttgg cgaacgggta caggttgatg atcagcaggt cgatggtgcc
     2221 gatgccgtgc tccttgagcg cggccatgtg ctcgggcacg tcgcggcgcg ccagcaggcc
     2281 gccgtgcacg cgcgggtgca gcgtcttgac gcgcccgtcc agcatctcgg gaaagccggt
     2341 gatctccgag acctcggtca cgggcaggcc gctctcggcc agcagcttgg ccgtgccgcc
     2401 ggtggacagc agcttcacgc cgctgtcatg cagggcgcgg gcgaagtcga cgatgccggt
     2461 cttgtcggat acggagagca gggcggtgag ggacatggcg tggtgcgggt caggatggaa
     2521 attcgaaagg ggacggcgcg ggtcgcgacg tcgcggtgtt tcagtggtcg acgagcttgt
     2581 gctcgagcag cttgcgccga agcgtgttgc ggttgatgcc cagccactcg gcggccttgc
     2641 tctggttgcc gtcggcgtgg cgcagcacga cgtccagcag cggcttctcg acggtgttca
     2701 tgaacatgtc gtgcatcgag tgcggctcga cgccgtgcag gtccttgaaa tactgttcca
     2761 gcgtctcgcg gacgcaggct tcgatctgtt tcttgctcat gcttggtctt gttgtcttcg
     2821 tgggagccgc caaccctcgc cggcggatcc tcaggcctct tgtcgaagca ggtcttcgtt
     2881 cgccgccggc cacagggtat ggcggtcggc cagctggtcg aagtagtcga tcacgcccgc
     2941 catctgcgtc ttgcagtcgg cgtgcgtgtt catcaccgcg cggaactccg gccctcccgg
     3001 caacgcgtgg acggcccagc cgatgtgctt gcgggccgtc cgcacgccca ggtcctctcc
     3061 gtagaggccg tagtggtcgt ggagatgttc ggtgagccag cgcgcgacgt cgcgcgtggc
     3121 cggcggggcg agcagctcgc ccgtcgacag gaaatgcgcg acctcgcgga agatccacgg
     3181 gcggccctgc gccgcgcggc cgatcatcag cgcgtcggcg ccggtggcct gcagcacttg
     3241 cagcgccttg tgaggggaat cgatgtcgcc gttggccacg acggggatgc gcagcgcggc
     3301 cttgacggcg gcgatggtgt cgtactcggc aaagcccttg tagccctgct cgcgcgtgcg
     3361 gccgtgcacg gtgaccatgg ccacgccggc gctctcggcg gcgcgcgcga tgcgcacggc
     3421 gttgcgctcg gcgtcgcacc agccggtgcg catcttgagc gtgacgggca cgttggccgg
     3481 cgcgcaggcg gcgaccacgg cctcgacgat ctccagcgcc agcggctcgt cctgcatcag
     3541 cgccgaaccg gcccacttgt tgcagacctt cttggccggg cagcccatgt tgatgtcgat
     3601 gatccgggcg ccgcggtcga tgttgtagcg cgcggcgtcg gccatcatct gcgcgtcggt
     3661 gccggcgatc tgcaccgaga tcggcgccgt ctcgccgtcg tggttggcgc ggcgcgaggt
     3721 cttgagcgtg tgccagaggt ccttgcggga cgtgaccatc tcgctgaccg cgtaccccgc
     3781 ccccagccgc ttgcacagca tgcggaacgg ccggtccgtc accccggcca tgggggcgac
     3841 gaacagggca ttcggcagct cgatcgcgcc aatgtgcatg gggtggatcc ggggaggcgg
     3901 tgggcagcga cctgctgtcg agccggtc
//
LOCUS       DATCRO010000426         6935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1005935, whole genome shotgun sequence.
ACCESSION   DATCRO010000426 DATCRO010000000
VERSION     DATCRO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005935"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VIN75_11525"
     CDS             complement(<1..1329)
                     /locus_tag="VIN75_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8974841.1"
                     /translation="MLKKPSRDQWITVAWIGGFVGLALIVSLLAAVVVVSQQLPELDE
                     ITHYQPRQPLEIVTREGIELAQFGPERRRFMPVGEFPDVLKNALIATEDADFRSHSGI
                     SIKGIARAAISNLFHHHRQGASTITQQVARNFFLSRKQSYSRKFAEALLALKIERHLS
                     KDQILELYMNQIYLGQHAYGFEAASLTYFGKPSRQLTAAEAAMLAGLPKNPGGADPTD
                     HFEKAKARQLVVLMRMHETGVLNDSDYEAAKKQPLKITGHWAPPVSAEYLAEMVRQQV
                     VQRFGEAAYSQGLKVTTSLVSADQAAAQAAVRKGVIDFDRRQAYRGPEDNEDLPDTDA
                     DIDSAAAAALHDHPDDDDLRVAVVTHASPKEVVAILASGETLHLSGDALRGVQAGLKP
                     KAAEDVAITRGAIIRVMRTTGDTSYKSAWRVTQWPDVEGALVSMDSATGQI"
     gene            complement(1473..2348)
                     /locus_tag="VIN75_11530"
     CDS             complement(1473..2348)
                     /locus_tag="VIN75_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8974842.1"
                     /translation="MSARTALRLVETWPGHDPAGAELADAPAAPRAPEPERPETAGAQ
                     TLRRGLAVLRLLTRVGPGGLRMGEIGHRLGLNKTTAIRLTRTLVDEGFVLHDRAAGRY
                     RLGPEAFAVGLAAEPSYELQRQAAPMLRALAADSGDTVFFTVLHGHESICLSRDEGDF
                     PIRNQLIKPGDRWPLGVGAGSAAILAALPDETVTDVLARNAALRAEKFPRCSDAAIRT
                     LVRDTRDRGYCLQPGMVFEDSWAIGVVVYDANEAPVASISIAALRSRLGPARSAMLGN
                     RLMQASKELTAQLKV"
     gene            complement(2345..3157)
                     /locus_tag="VIN75_11535"
     CDS             complement(2345..3157)
                     /locus_tag="VIN75_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8974843.1"
                     /translation="MTTSTSINADSPVALVTGGARGIGLAIGQWFLARGHRIALLDID
                     DETLQRTVAAFDNPDAVLAVHADVKAPEQVAAGVAQVVAKFGRIDKLVNNAGIAVFKP
                     VMQTSYAEFRDVLATNLDGAFICTQACVPHMRKSGKGAIVNIASISGLRASTLRVAYG
                     TSKAALLHMTKQLAVELGDVGIRVNAICPGPVETEMAKLVHSVAIRSDYHDAIPLGRY
                     GTPEEMANTVGFLCSDDASFINGQFLAVDGGFDAAGVGLPTLRRNAAAASAQ"
     gene            complement(3300..3689)
                     /locus_tag="VIN75_11540"
     CDS             complement(3300..3689)
                     /locus_tag="VIN75_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY8974844.1"
                     /translation="MSEVRLVEAGAMGRVAYARVAPNEDLVLSIEKLCVAEGFRHAFV
                     RGALGSLVDACFVAPGGGTRLVRGPAVEIVSLAGEVRTQADGSLRAAITGIVADTEGH
                     VHGGPFVAGANPVCVTFEVTLEEWLPS"
     gene            complement(3682..4257)
                     /locus_tag="VIN75_11545"
     CDS             complement(3682..4257)
                     /locus_tag="VIN75_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEY8974845.1"
                     /translation="MTDFLTTQPLPRPRTLVHPGPVGPIRIEHRHASLGRHFRLGLAP
                     GRTLHDAIIEPLLRLGVRSASMTLLGGRLSTLLYCVAPPDPTGERVANYTRPIEAGAA
                     TLIFGNATLGWNGRGEPLVHCHAAFACDDGRVRGGHIVVDRSIAESPVPVLATSLDGI
                     ELRITQDDETHMPLMRPFAAAVQRIEETSHV"
     gene            4405..5289
                     /locus_tag="VIN75_11550"
     CDS             4405..5289
                     /locus_tag="VIN75_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8974846.1"
                     /translation="MRHLRCLVAVAEELHFGRAAARLNLSQPPVSLAIKELEAELGVT
                     LFERSSRRIVLTREGEEAVRDARAVLARTELLKRRAHDAAQGQSGTLSIGFISLAAYS
                     FLPDTLRRFMADFPKVKLALHESTTDAMLAELEIGALDIGLIFASPGMAPTLAYRATQ
                     REALMIALPGHHPQAKSSQVALEKLAHERFLGFERHYGPMIFDAMVATCMRHGFSPDL
                     FPARQMHTIVSLVSGGIGVALVPASVKALHREGVVYRPIKGERTLVETGAAWRKADDS
                     VLVQAFVEYLPRLSAARG"
     gene            5971..>6935
                     /locus_tag="VIN75_11555"
     CDS             5971..>6935
                     /locus_tag="VIN75_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8974847.1"
                     /translation="MGFRYFLGIDVAKAKLDCVLLDSSEDRRRSKVVPNDPEGFQALV
                     KWLAKQGVGCAHVHAILEPTGVYHERALFALAQAGMAVSLVNPAQLRRFAQGLGIHTK
                     TDACDSAVLARFGQMAKPAPWQPAPLKARELRALLARRDAVAQDLQRERNRAEQLVVG
                     EAPQRVLQSLAEAISFLENELQALQRAIDQHIDDDPDLKRIWELLQSIPGVGQRVADH
                     FTALLSGRDFTSAEQLAAYLGLVPTEWQSGSSIKGRPRMSKAGSASLRQLLYMPAVVA
                     IRHNPHVKALYERLLARGKAKMSALGAAMHKLAQLCFGVVHTGQP"
BASE COUNT          982 a         2325 c         2499 g         1129 t
ORIGIN      
        1 gatctggccg gtggccgagt ccatcgacac cagcgcgcct tccacgtccg gccactgcgt
       61 gacgcgccac gcgctcttgt agctggtgtc gcccgttgtg cgcatcacgc ggatgatggc
      121 gccgcgcgtg atcgcgacgt cctccgccgc cttgggcttc aggccggcct gcacgccgcg
      181 cagcgcgtcg ccactcaggt gcagcgtctc gccgctggcc aggatcgcga ccacttcctt
      241 cgggctcgcg tgcgtgacga cggccacgcg caggtcgtcg tcgtcggggt ggtcgtgcag
      301 ggcggcggcc gcggcgctgt cgatgtcggc gtcggtgtcg ggcaggtcct cgttgtcttc
      361 cggaccgcgg tacgcctggc ggcggtcgaa gtcgatcacg cccttgcgca cggcggcctg
      421 cgcggcggcc tggtcggccg acaccagcga ggtcgtcacc ttcaggccct gcgagtacgc
      481 cgcctcgccg aagcgctgca ccacctgctg gcgcaccatc tcggcgagat attcggcgct
      541 caccggtggc gcccagtggc cggtgatctt cagcggctgc ttcttcgcgg cctcgtagtc
      601 ggagtcgttg agcacgcccg tctcgtgcat gcgcatcagc acgaccagct ggcgcgcctt
      661 ggccttctcg aagtggtcgg tggggtcggc gccgcccggg ttcttgggca ggccggccag
      721 catcgcggcc tcggcggcgg tgagctgcct ggacggcttg ccgaaatagg tgagcgaggc
      781 cgcctcgaag ccgtacgcgt gctggcccag gtagatctgg ttcatgtaca gctcgaggat
      841 ctggtccttc gagagatggc gctcgatctt gagcgcgagc agcgcctcgg cgaacttgcg
      901 cgagtacgac tgcttgcggc tcaggaagaa gttgcgcgcg acctgctgcg tgatggtcga
      961 ggcgccctgg cgatggtggt ggaacaggtt ggagatcgcg gcgcgcgcga tgcccttgat
     1021 gctgatgccg ctgtggctgc ggaagtcggc gtcctcggtg gcgatgagcg cgttcttgag
     1081 cacgtcgggg aactcgccga ccggcatgaa gcggcggcgc tcggggccga actgcgcgag
     1141 ctcgatgcct tcgcgcgtga cgatctccag cggctggcgc ggctggtagt gggtgatctc
     1201 gtcgagttcg ggcaattgct gcgagacgac gacgacggcg gcgagcagcg aaacgatgag
     1261 cgcgaggccg acgaaaccgc cgatccatgc gacggtgatc cactggtccc gggaaggctt
     1321 cttgagcacg gtgcaggccg ccgcggcggc agagcagaca agacgccgac tttaattgac
     1381 ggcaggcgcc gccccgtgtc aggacgtacc gtgctggacg ggtggcgttg aaagtatcgt
     1441 tcggcgcgcc ctcgggacgc gtcggctgcc gctcagacct tcaactgcgc ggtgagttcc
     1501 ttgctcgcct gcatcagccg gttgcccagc atcgcgctgc gcgccgggcc caggcgggag
     1561 cgcagggccg cgatgctgat cgaggccacc ggcgcctcgt tggcgtcgta gacgaccacg
     1621 ccgatcgccc agctgtcctc gaacaccatg cccggctgca ggcagtagcc gcggtcgcgc
     1681 gtgtcgcgca ccagcgtgcg gatcgcggcg tcgctgcagc gcgggaactt ctcggcgcgc
     1741 agcgcggcat tgcgcgccag cacgtcggtg acggtctcgt cgggcagcgc ggccaggatc
     1801 gcggcgctgc cggcgcccac gcccagcggc cagcggtcgc ccggcttgat gagctggttg
     1861 cggatcggga agtcgccctc gtcgcgcgac aggcagatgc tctcgtggcc gtgcagcacg
     1921 gtgaagaaca cggtgtcgcc ggagtccgcc gccagcgccc gcagcatcgg cgcggcctgg
     1981 cgctgcagct cgtagctggg ctcggcggcc aggccgacgg cgaaggcctc gggccccagc
     2041 cggtaacggc ccgcggcgcg atcgtgcagc acgaagccct cgtcgaccag cgtgcgcgtc
     2101 aggcggatcg cggtcgtctt gttcaggccc agccggtggc cgatctcgcc catgcgcagg
     2161 ccgcccgggc ctacgcgcgt gagcaggcgc agcaccgcga ggccgcggcg cagcgtctgc
     2221 gcgccggcgg tctcgggccg ctcgggctcg ggcgcgcgag gcgcggccgg cgcatcggcc
     2281 agctcggcgc cggccgggtc gtggcccggc cacgtctcga ccaggcgcag ggcagttcgt
     2341 gcgctcattg ggcggacgcg gccgcggcgt tgcggcgcag cgtgggcagg ccgacgccgg
     2401 cggcgtcgaa gccgccgtcc accgcgagga actggccgtt gatgaagctt gcgtcgtcgc
     2461 tgcacaggaa cccgaccgtg ttggccatct cctcgggcgt gccgtagcgg cccagcggga
     2521 tcgcgtcgtg gtagtcggag cggatcgcca cgctgtgcac cagcttggcc atttccgtct
     2581 cgaccgggcc cggacagatc gcgttgacgc ggatgccgac gtcgcccagt tcgacggcca
     2641 gctgcttggt catgtgcagc agcgcggcct tgctggtgcc gtaggccacg cgcagcgtgc
     2701 tggcgcgcag gcccgagatc gacgcgatgt tgacgatcgc gcccttgccc gacttgcgca
     2761 tgtgcggcac gcaggcctgg gtgcagatga aggcgccgtc gaggttcgtg gcgagcacgt
     2821 cgcggaactc ggcgtagctg gtctgcatca ccggcttgaa gacggcgatg ccggcattgt
     2881 tcacgagctt gtcgatgcgg ccgaacttcg ccaccacctg cgcgacgccc gcggcgacct
     2941 gctcgggggc cttcacgtcg gcgtgcacgg cgagcaccgc gtccgggttg tcgaacgcgg
     3001 cgacggtgcg ctgcagcgtc tcgtcgtcga tgtccagcag cgcgatgcgg tggccgcggg
     3061 caaggaacca ttggccgatg gccaggccga tgccgcgcgc gcctccggtg acgagggcga
     3121 cgggcgagtc ggcgttgatc gaagtggacg tggtcaaggt ggtctccaga ggcatgaatg
     3181 gcgcgggcgg tcgccgcgtc ggtttcagag tgatgggccg ccgctcaagc cggcgcgcgg
     3241 ccgctcagga gcggcctgag cgccccctcg cggggctgct cgcagcgagc tcggggtcgt
     3301 catgacggca accactcttc gagcgtcacc tcgaacgtga cgcacacggg attggcgccg
     3361 gcgacgaacg gtccgccgtg cacgtggccc tcggtgtcgg cgacgatgcc ggtgatggcc
     3421 gcgcgcagcg agccgtcggc ctgcgtgcgc acctcgccgg ccaggctcac gatctcgacg
     3481 gccgggccgc gcacgaggcg cgtgccgccg ccgggcgcga cgaagcaggc gtcgaccagg
     3541 ctgccgagcg cgccgcgcac gaacgcgtgg cggaagccct cggcgacgca cagcttctcg
     3601 atgctcagca ccaggtcctc gttcggggcc acgcgcgcat aggcgacgcg gcccatcgcg
     3661 ccggcctcga ccaggcggac ttcagacatg gctcgtctcc tcgattcgtt gcaccgcggc
     3721 cgcgaacggg cgcatcagcg gcatgtgggt ctcgtcgtcc tgcgtgatgc gcagctcgat
     3781 gccgtccagc gaggtcgcca gcaccgggac cggcgactcg gcgatgctgc ggtcgaccac
     3841 gatgtggccg ccgcgcaccc ggccgtcgtc gcaggcgaac gcggcgtggc aatgcaccag
     3901 tggctcgccg cggccgttcc agcccagcgt cgcattgccg aagatcagcg tggccgcgcc
     3961 ggcctcgatc gggcgcgtgt agttcgcgac gcgttcgccg gtcggatccg gcggggccac
     4021 gcagtacagc agcgtcgaca ggcggccgcc gagcaaggtc atcgacgctg aacggacgcc
     4081 caggcgcagc aggggttcga tgatcgcgtc gtgcagcgtg cgaccgggcg cgaggccgag
     4141 gcggaaatgg cggccgaggc tcgcatgccg gtgctcgatg cggatcgggc cgacggggcc
     4201 cgggtgcacc agcgtccggg ggcggggcag cggctgcgtg gtcaggaagt cggtcaatcg
     4261 tgtctccggc aggttcgtgg cccgcatcgt ttcctcttgc caccgggttt cggcggtggg
     4321 agtcctggcg cagtctggca aagggctttc attcggggaa gttttcattt cagatcgatt
     4381 gatccagaat tcagatcgat ggaaatgcgt catctccgct gcctggtggc cgtcgccgag
     4441 gaactgcact tcgggcgcgc ggcggcgcgg ctgaacctgt cgcagccgcc ggtgagcctg
     4501 gcgatcaagg agctcgaggc cgagcttggc gtcacgctgt tcgagcgcag ttcgcgccgc
     4561 atcgtgctca cgcgcgaggg cgaggaggcc gtgcgcgacg cccgcgcggt gctcgcgcgc
     4621 accgagctgc tcaagcgtcg cgcgcatgat gcggcgcaag gacagtcggg cacgctgtcg
     4681 atcggcttca tcagcctggc ggcctattcg ttcctgcccg atacgctgcg ccgcttcatg
     4741 gccgacttcc cgaaggtcaa gctggcgctg cacgagtcca ccaccgacgc gatgctcgcg
     4801 gaactggaga tcggcgcgct ggacatcggc ctgatcttcg cgtcgccggg catggcgccc
     4861 acgctcgcct accgcgcgac gcagcgcgag gcgctgatga tcgcgctgcc cggccatcat
     4921 ccgcaggcga agtcgtcgca ggtggcgctg gagaagctcg cgcacgagcg cttcctcggc
     4981 ttcgagcgtc actacggccc gatgatcttc gatgcgatgg tggccacctg catgcgccac
     5041 ggcttcagtc ccgacctgtt cccggcgcgg cagatgcaca ccatcgtcag cctcgtctcg
     5101 ggcgggatcg gcgtggcgct ggtgccggcg tcggtcaagg cgctgcaccg cgagggcgtt
     5161 gtctaccggc cgatcaaggg cgagcgcacg ctcgtggaga ccggcgccgc ctggcgcaag
     5221 gccgacgatt ccgtgctggt gcaggccttc gtcgagtact tgccgcggct gtccgccgcg
     5281 cgcggctgag cgatcgcgca gcgcgctgcg atcgaagccc agcccaccct gccgcgccgg
     5341 cgtgtgagct gttggagaaa aacattcgcg tttttcgaaa ggggctttgg catcgacggg
     5401 ccatcgaggc cgcgtcgatg cccgcggcca gcgctcttgc ggggtggcgg gcttgacgcg
     5461 gatcgcgggc caccgtgtcg gcgctccttc tcgtgtccga cgaccacgcc gccctcggcg
     5521 tcgtggcgtc ggcctgcgct gtgtggccgc gcggccgtcc ggcgtcaggc gacgtcaagc
     5581 caacttggca acggcttcga ggcatgcttt cgacccgttg cggaaatcgg tgagcgcaga
     5641 tagcttccat cagagcggac ctcgtagggc gggttagccg caggcgtaac ccgccgcttg
     5701 cagcccgcgc gacagatggc gggttacgcc tgcggctaac ccgcccttgt atctttacgt
     5761 ccgtggtgga gtcgctcccc gccacatgag gagcgcgagc ccgaacccgc ccttgggctt
     5821 cgagcccgtt gcgtagatgg ggccgcgtct cctcgcctgt tgcacctggc aagacactga
     5881 acggtagcca agggcctgga ccccgcatgt acaaggcgag tgggtgtgcg gggtgcaagg
     5941 caaatgcatt gggagcgcaa ggagtgcatg atgggatttc gctactttct gggcatcgac
     6001 gtcgccaagg ccaagctcga ctgcgtcttg ctcgactcgt ctgaagatcg ccgccgctcc
     6061 aaggtcgttc ccaacgaccc tgaaggcttt caagccctgg tcaagtggct ggccaagcaa
     6121 ggggtgggct gtgcccacgt gcatgcgatc ctggagccca cgggtgtcta tcacgagagg
     6181 gcattgttcg cgttggcgca agccggcatg gcggtgtcct tggtcaaccc agcccagttg
     6241 cgtcgctttg cacagggcct gggaatacac accaagacgg atgcctgcga cagcgcggtg
     6301 ctcgcacgct ttggtcaaat ggccaagccc gcgccttggc agcccgcgcc cttgaaggcg
     6361 cgtgagttgc gggcgctgct ggcgcgccgc gacgctgtgg cccaagacct gcagcgcgaa
     6421 cgcaatcggg ccgagcaatt ggtggtcggt gaagctcccc agcgtgttct gcagtcgttg
     6481 gccgaggcga tctcgttcct cgaaaacgag ctgcaggccc tacagcgggc catcgaccag
     6541 cacatcgacg atgacccgga tctgaagcga atctgggaac tgctccagag cattcctgga
     6601 gtcggccaac gcgtggccga ccacttcacg gcgttgctgt ccggacgcga cttcacgtcc
     6661 gccgagcaac tggccgccta ccttggtctg gttcccaccg agtggcaatc gggctcgtcg
     6721 atcaagggcc gtccacgcat gagtaaggct ggctcggcat cgctgcgcca gctgctctac
     6781 atgcccgcgg tcgtcgccat tcgccacaac ccgcacgtca aggcgcttta cgagcggctg
     6841 ttggcccgcg gcaaggccaa gatgagtgcc ctcggcgccg ccatgcataa gctcgcgcaa
     6901 ttgtgcttcg gcgtcgttca cacaggccag cccta
//
LOCUS       DATCRO010000427         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_533543, whole genome shotgun sequence.
ACCESSION   DATCRO010000427 DATCRO010000000
VERSION     DATCRO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533543"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VIN75_11560"
     CDS             complement(<1..829)
                     /locus_tag="VIN75_11560"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY8974848.1"
                     /translation="MTRTTSTHSTLKLAITALLATMAAERALAQDAAAPAASAASAAA
                     PAAAASGAAAPLDASGSPRTAAGEKDFTQTQQVVVLGNSRREGMLKKEAGYSITTADA
                     EQIKDAAPTSTADLLKIVPGVFVETTGGESGANIRVRGFPEAGDSPYATIQMNGSPIF
                     AAPSLSFLEGSSLFRIDDTIERVEVLRGGTSPIYGSGQPGVSVNFVQRKGNDAPEGDI
                     RLTAGSGNLRRLDGYWSGPIATNWFATVGGFYDTSNGLRDTQFPSDRGGQVSALITHK
                     "
     gene            complement(1091..2209)
                     /locus_tag="VIN75_11565"
     CDS             complement(1091..2209)
                     /locus_tag="VIN75_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974849.1"
                     /translation="MRFGAVDVLRAIDLRIAPGEFCVFVGPSGCGKSTLLRLVAGLED
                     PSAGEIHMRGVRVDLLPPAGRDIAMVFQSYALYPHMTVRQNMAFGLEYRKLDDAEVRR
                     RVGAASAMLQLDALLDRKPRDLSGGQRQRVAIGRALVRQPSVFLFDEPLSNLDAALRV
                     QTRVEIARLHRELGTAAMVYVTHDQVEAMTLADRIVLLRPLAGAAGGAASIAQVGAPM
                     DLYHRPANRFVAGFIGSPAMNFLPAVALDIGADGLALRVAGADLRALVDPPAPARSAE
                     VTAGIRPEHVRLDDGAALRAAVTHVECLGEHAWVYLRLPDGGAIVAKSVDDVLAPGDQ
                     VGVSFPAEHVHVFGADDVAFARRMAPVATARRDAVPVR"
     gene            complement(2251..3921)
                     /locus_tag="VIN75_11570"
     CDS             complement(2251..3921)
                     /locus_tag="VIN75_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HEY8974850.1"
                     /translation="MTFSRADADWWRGAVIYQIYPRSFQDSNGDGIGDLAGIVRRLPY
                     VAALGVDAIWVSPFFPSPMRDFGYDISDYCDVDPRFGTLADLDRLVETAHSLGLKVLI
                     DLVLSHSSSAHPWFAESRSSRDNAKADWYVWANASPDGTPPNNWLSLFGGSAWQWDTG
                     RGQYYLHNFLASQPDLNYHCPEVRAAALDVARFWLARGIDGFRLDVVNFYFHDRHLRS
                     NPPQPLGRFAAAVPPSNPYAFQSHVHDKTQPENLEFLHELRALVDEVPGRTMIGEIGD
                     DNEYHTLGEYTREGRLHMAYTFNLMGTDCTPLFIHGVLDEFLRETRDAWCCWALSNHD
                     VVRVATRWRALGEPQTLAPLLLAFMGSLRGSVCLYQGEELGLSEAVIDFEDLQDPYGK
                     EMWPLFKGRDGCRTPMPWSTRQVHAGFSDAAPWLPMAPDHRSRAVDDQEREPYSVLAR
                     YRGFLAFRRGQAALLRGSMHLLPEHEQVLAWVRQDGDDAVLCIFNLSAHPARYPLRAC
                     DVVALDGHGFDGEMERDADGLSHACVMPPNGAWFGRLRSPETLTEALP"
     gene            complement(3974..>4474)
                     /locus_tag="VIN75_11575"
     CDS             complement(3974..>4474)
                     /locus_tag="VIN75_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8974851.1"
                     /translation="ASMFPQVAVLSGMFELVRALGLYDSLGALIVSDLMLTLPFTVWV
                     LVTFMRQLPRELEEAAAMDGLGPLTLVFRIFLPLLWPALVATGLLAFIAAWNEFLFAL
                     TFTLSATSRTVPVAIALISASSAFELPWGGIMAASVIVTLPLVGLVLLLQRHIVSGLT
                     QGAVKG"
BASE COUNT          670 a         1520 c         1682 g          602 t
ORIGIN      
        1 gcttgtgggt gatcagcgcg ctgacctgcc cgccacggtc ggacgggaac tgcgtgtcgc
       61 gcaggccgtt gctggtgtcg tagaagccgc ccaccgtggc gaaccagttg gtggcgatcg
      121 ggcccgacca gtagccgtcc aggcgccgca ggttgcccga gccggcggtc aggcggatgt
      181 ccccctcggg cgcgtcgttg cccttcctct gcacgaagtt gaccgacacg ccgggctggc
      241 ccgagccgta gatcgggctg gtgccgccgc gcagcacctc cacgcgctcg atggtgtcgt
      301 cgatgcggaa cagcgacgag ccctcgagga aggacagcga cggcgccgcg aagatcggcg
      361 agccgttcat ctggatggtc gcatagggcg aatcgcccgc ctcgggaaag ccgcgcacgc
      421 ggatgttggc gcccgactcg ccgccggtgg tctcgacgaa cacgcccgga acgatcttga
      481 gcaggtcggc cgtcgaggtc ggtgcggcgt ccttgatctg ctcggcgtcg gccgtggtga
      541 tggagtagcc ggcttccttc ttgagcatgc cctcgcgccg cgagttgccc aggacgacga
      601 cctgctgcgt ctgcgtgaag tccttctcgc cggcggccgt gcgcgggctg ccggacgcgt
      661 ccagcggcgc ggcggcgccg gaggcggcgg cggccggcgc ggccgcggag gcggccgagg
      721 ccgcaggggc ggcggcgtcc tgcgcgagcg cgcgttcggc ggccatcgtg gccagcaacg
      781 cggtgatcgc gagcttcaga gtgctgtgag tcgatgttgt tcgggtcatg tcgttgtcgc
      841 cctaccgtga gttgtccatc cggtcggcgg acgttcgcgg ggaacgtccg tgccggctcc
      901 gtgggaagcg ccggtccgcg ggggcggccg tcgtctgcct ggcgccgggc ttgtctcctg
      961 cccgccgcct ggaatcaatg tagacagcca gatgcatcgg cgaatgtcgt tcttcgaggc
     1021 tgcgcaaaaa cttgatttca attcggcggc gcgcatgaaa cggatgaaac acgccggacg
     1081 tccgcagcgg tcagcgcacc gggaccgcgt cgcgccgcgc ggtcgccacg ggcgccatgc
     1141 ggcgcgcgaa cgcgacgtcg tcggcgccga agacatgcac gtgctccgcg gggaacgaca
     1201 cgcccacctg gtcgcccggt gcgagcacgt cgtccacgga cttggccacg atcgcgccgc
     1261 catcgggcag gcgcaggtag acccaggcgt gctcgccgag gcactcgacg tgcgtgaccg
     1321 cggcgcgcag cgcggcgccg tcgtccaggc gcacgtgctc gggccggatg cccgcggtga
     1381 cctcggcgga acgggcgggc gccggcggat cgaccagcgc gcgcaggtcg gcgcccgcca
     1441 cgcgcagcgc caggccgtct gcgccgatgt ccagcgccac ggcgggcagg aagttcatcg
     1501 cgggcgagcc gatgaagccg gccacgaagc ggttggcggg ccggtggtac aggtccatcg
     1561 gcgcgccgac ctgcgcgatg ctcgcggcgc cgccggccgc gccggccagc gggcgcagca
     1621 gcacgatgcg gtcggccagc gtcatcgcct cgacctggtc gtgcgtgacg tagaccatcg
     1681 cggcggtgcc cagctcgcgg tggagccgcg cgatctcgac gcgggtctgc acgcgcagcg
     1741 cggcatcgag gttggacagc ggctcgtcga acaggaacac cgagggctgg cgcaccagcg
     1801 cccgcccgat cgccacgcgc tggcgctgcc cgcccgacag gtcgcgcggc ttgcgatcga
     1861 gcagcgcatc cagttgcagc atcgcgctgg ccgcgccgac gcgccggcgc acctcggcgt
     1921 cgtcgagctt gcggtattcg agcccgaagg ccatgttctg gcgcacggtc atgtgcgggt
     1981 acagcgcgta gctctggaac accatggcga tgtcgcgccc ggcaggcggc agcaggtcga
     2041 cgcgcacgcc gcgcatgtgg atctcgcccg cgctgggatc ctcgagtccc gcgaccaggc
     2101 gcagcagcgt ggacttgccg cagcccgacg gcccgacgaa gacgcagaat tcgccgggcg
     2161 cgatgcgcag gtcgatcgcg cgcagcacgt cgaccgcgcc gaagcgcatg ctgacgccgc
     2221 gcaggtcgat ggcgggcgcg tcggtcgtgt tcatggcagg gcctccgtca aggtctcggg
     2281 cgatcgcagg cggccgaacc aggcgccgtt gggcggcatc acgcaggcgt gcgacaggcc
     2341 gtcggcgtcg cgttccatct ccccgtcgaa gccgtggccg tcgagcgcga cgacgtcgca
     2401 ggcccgcagc ggataccgcg ccggatgcgc ggacaggttg aagatgcaca gcaccgcgtc
     2461 gtcgccgtcc tggcgcaccc aggccagcac ctgctcgtgc tcgggcagca ggtgcatcga
     2521 cccgcgcagc agcgcggcct ggccgcggcg gaacgcgagg aagccgcggt agcgcgcgag
     2581 caccgagtac ggttcgcgct cctggtcgtc gaccgcgcgc gaacggtggt cgggcgccat
     2641 gggcagccag ggcgcggcgt cgctgaagcc ggcgtgcacc tggcgcgtgg accacggcat
     2701 gggcgtgcgg cagccgtcgc ggcccttgaa cagcggccac atctccttgc cgtacggatc
     2761 ctgcaggtcc tcgaagtcga tgacggcctc ggacagcccc agctcctcgc cctggtacag
     2821 gcagaccgag ccgcgcagcg agcccatgaa cgcgagcagc agcggagcga gcgtctgcgg
     2881 ctcgccgagc gcgcgccagc gcgtggccac gcgcacgacg tcgtggttgc tcagcgccca
     2941 gcagcaccac gcgtcgcgcg tctcgcgcag gaactcgtcg agcacgccgt ggatgaacag
     3001 cggcgtgcag tcggtgccca tcaggttgaa ggtgtaggcc atgtgcagcc ggccctcgcg
     3061 cgtgtactcg cccagcgtgt ggtactcgtt gtcgtcgccg atctcgccga tcatggtgcg
     3121 gccgggcacc tcgtcgacca gcgcgcgcag ctcgtgcagg aactcgaggt tctcgggctg
     3181 cgtcttgtca tggacgtggc tctggaacgc gtacgggttg ctgggcggca ccgccgcggc
     3241 gaagcggccg agcggctgcg gcgggttcga gcgcaggtgg cggtcgtgga agtagaagtt
     3301 gacgacgtcg agccggaagc cgtcgatgcc gcgcgcgagc cagaagcgcg cgacgtcgag
     3361 cgcggcggcg cgcacctcgg ggcagtggta gttcaggtcc ggctgcgacg cgaggaagtt
     3421 gtgcaggtag tactggccgc gtccggtgtc ccattgccac gcgctgccgc cgaacagcga
     3481 cagccagttg ttgggcggcg tgccgtcggg cgacgcgttg gcccacacgt accagtcggc
     3541 cttggcgttg tcgcggctgg aacggctctc ggcgaaccag ggatgcgccg aggacgagtg
     3601 gctcagcacg aggtcgatca gcaccttcag gcccagcgag tgcgcggtct cgacgaggcg
     3661 gtccaggtcg gccagcgtgc cgaagcgcgg gtcgacgtcg cagtagtcgg agatgtcgta
     3721 gccgaagtcg cgcatcggcg acgggaagaa cggcgacacc cagatcgcgt cgacgcccag
     3781 cgcggccacg tagggcaggc ggcgcacgat gccggccagg tcgccgatgc cgtcgccgtt
     3841 gctgtcctgg aagctgcgcg ggtagatctg gtagatcacg gcgccgcgcc accagtcggc
     3901 atcggcgcgg gagaaggtca tgctcatcgc gttggagaat gtgcgggaag ggacggcgcg
     3961 gcggcgtcgg gcgtcagccc ttgaccgcgc cctgggtgag gccggagacg atgtggcgct
     4021 gcagcagcag caccaggccc acgaggggca gcgtgacgat gaccgacgcg gccatgatcc
     4081 cgccccaggg cagctcgaac gcggacgacg cgctgatcag cgcgatggcc accggcaccg
     4141 tgcgcgacgt ggccgacagc gtgaaggtga gcgcgaacag gaattcgttc cacgccgcga
     4201 tgaacgccag caggccggtg gccaccagcg cgggccacag cagcggcagg aagatgcgga
     4261 agaccagcgt gagcggcccg aggccgtcca tcgcggcggc ctcctccagc tcgcgcggca
     4321 gctggcgcat gaaggtgacc agcacccaca cggtgaacgg cagcgtgagc atcaggtcgg
     4381 acacgatcag cgcgcccagg ctgtcgtaca ggcccagcgc gcgcacgagc tcgaacatgc
     4441 ccgacagcac cgcgacctgc gggaacatcg acgc
//
LOCUS       DATCRO010000428         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_837319, whole genome shotgun sequence.
ACCESSION   DATCRO010000428 DATCRO010000000
VERSION     DATCRO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837319"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VIN75_11580"
     CDS             <1..800
                     /locus_tag="VIN75_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8974852.1"
                     /translation="AGTVCRLPDGFATVLDAALRIAAASGGAYDPSAGALVALWGFGP
                     SGETALAPRHDEASFRSPSAQDCARVHCGWDALPWDGERRTLVQTGGVALDLSAIAKG
                     FAVDRVSDVLGALGLPDHLVEIGGELRGAGLKPDGQPWWVDLEPPAADCGLAPLRIAL
                     HGLSVATSGDYRKCFADADGQWRSHTIDPRTRRPVEHGMASVTVVHDSAMAADGWSTA
                     LMVLGPDEGLALADRLGLAARLVRRRPSIEGGGFEERFSTAMRALML"
     gene            800..2239
                     /locus_tag="VIN75_11585"
     CDS             800..2239
                     /locus_tag="VIN75_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY8974853.1"
                     /translation="MAAILSRPAAALLLLALYLLMCAAVWWRHRRRRRQAAEESAALL
                     PAADAGPPVLVLHASQTGQAEEIAWQTARALHLAGMPVRVAALGEVDRDALRGARHAL
                     FIASTYGEGDAPDSAAPFARDLMAAEASDAAGGLDLSSLQVGVLALGDATYTHFCGFG
                     RALDAWLRERGARSMFDRIEADRADEHALSQWRQRLSHLAGTADLAEWEAPVFTSWRL
                     AGRRHLNPGSQGGPVFHLELVPADGSSLPAWEAGDLVQVSVAGEAADAIDEDARRPRE
                     YTIASLPADGHVELMVRRTAREDGTPGLASGWLTQRLAVGGGVALRLRPHASFRIGAN
                     TDRPLILVGNGTGLAGLRAHLKARSASRMNTGIGGAAPAWLFFGERQSAHDAHYRDEI
                     EAWQRDGVLARVDWAFSRDQAERVHVQHLLAREAPRLREWVAEGAAIYVCGSLQGMAG
                     AVDAVLRETLGEADVDGLIREGRYRRDVY"
     gene            2525..2944
                     /locus_tag="VIN75_11590"
     CDS             2525..2944
                     /locus_tag="VIN75_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY8974854.1"
                     /translation="MATAKKAAPAKKAAPAKKAAPAKKAAAPAKKAAPAKKAAPAKKA
                     APAKKAAAPAKKRTPNAAFMKALTPSPALAAIVGDKPLPRTDVVKKVWEYIKKHNLQD
                     AAQKRVINADAKLKEIFGKAKADMFEMTRLINSHLKD"
     gene            complement(3047..>3429)
                     /locus_tag="VIN75_11595"
     CDS             complement(3047..>3429)
                     /locus_tag="VIN75_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8974855.1"
                     /translation="AMSSPTAPRHVLYVEDDRINIVLMEEVFRLMPGWTLDVAETGAQ
                     AMEMLPRVMPSLVLMDMNLPDMNGLELMALVRADARLAQLRCIALSADVMDDQRQRAR
                     AAGFQDYWLKPIDVRQLRAALERL"
BASE COUNT          493 a         1178 c         1278 g          480 t
ORIGIN      
        1 ccgccggcac ggtgtgtcgg ttgcccgacg gcttcgcgac ggtgctcgat gcggcactgc
       61 gcatcgcggc cgccagcggt ggcgcctacg acccgagcgc tggcgcgctg gtggcgctgt
      121 ggggcttcgg tccctcgggc gagacggcgc tcgcgccgcg ccacgacgag gcgagcttcc
      181 ggtcgccgtc cgcccaggat tgcgcccgcg tccactgcgg ctgggacgcg ctgccctggg
      241 acggtgagcg tcgcacgctg gtgcagaccg gcggcgtcgc cctggacctg tcggcgattg
      301 ccaagggctt cgccgtcgac cgcgtgtccg acgtgctggg cgcgctgggc ctgccggatc
      361 acctggtcga gatcggcggg gaactgcgcg gcgccggcct gaagccggat ggacagccct
      421 ggtgggtcga cctcgagccg cccgcggccg actgcgggct ggcgccgctg cgcatcgcgc
      481 tccacggcct gtcggtcgcg acctcgggcg actaccgcaa atgctttgcc gacgccgatg
      541 ggcaatggcg ctcccacacc atcgatccgc gcacgcggcg accggtcgag cacggcatgg
      601 cgtcggtcac tgtggtgcat gacagcgcga tggccgccga cggctggtcg accgcgctga
      661 tggtgctcgg tcccgacgag gggctcgcgc tggccgaccg gctcggcctg gccgcacggc
      721 tcgtgcggcg ccgcccgtcg atcgagggcg gcggtttcga ggagcgcttc tccaccgcga
      781 tgcgcgcgct gatgctctga tggctgcgat cctgtctcgt cccgcggcgg cgctgctgct
      841 gctcgcgctc tacctgctga tgtgcgccgc cgtctggtgg cggcatcgcc gccggcgacg
      901 ccaggcggcc gaggaatccg ccgcgctgct gccggccgcc gacgccggtc cgccggtgct
      961 ggtgctgcat gcgagccaga cgggccaggc cgaggagatc gcctggcaga ccgccagggc
     1021 gctgcacctg gccggcatgc cggtgcgcgt cgccgcgctc ggcgaggtgg atcgcgatgc
     1081 gctgcgtggc gcgcggcacg cgctgtttat cgccagcacc tacggcgaag gcgacgcgcc
     1141 cgacagcgcg gcgccgttcg cgcgcgacct gatggcggcg gaagcgtcgg acgcggccgg
     1201 cggcctcgac ctgtcctcgc tgcaggtggg cgtgctggcg ctgggcgatg ccacctacac
     1261 ccacttctgc ggtttcggac gcgcgctcga cgcctggctg cgcgagcgcg gcgcgcggtc
     1321 gatgttcgat cgcatcgagg ccgatcgggc cgatgaacac gcgctgtcgc agtggcggca
     1381 acgcctgagt cacctggccg gcaccgccga cctggccgag tgggaagcgc cggtgttcac
     1441 gtcctggcgc ctggccgggc gccggcatct gaacccgggc agtcaaggtg gaccggtgtt
     1501 ccacctcgag ctggtgccgg cggacggatc gtcgctgccc gcctgggagg ctggcgatct
     1561 cgtgcaggtg agcgtcgccg gcgaagccgc cgatgcgatc gacgaggacg cgcggcggcc
     1621 gcgcgaatac acgatcgcgt cgctgcctgc ggacggccat gtcgagctga tggtgcgccg
     1681 caccgcgcgc gaggacggca cgccgggcct ggcgtcggga tggctgacgc agcggctcgc
     1741 cgtcggcggc ggcgtcgcgc tgcgcctgcg tccgcatgcg agcttccgca tcggcgcgaa
     1801 caccgaccga ccgctgatcc tcgttggcaa cggcaccggg ctggcgggat tgcgcgcgca
     1861 tctgaaggct cgctcggcaa gccgcatgaa cacggggatc ggcggcgccg cgccggcatg
     1921 gttgttcttc ggtgaacggc agtcggcgca cgacgcgcat taccgcgacg agatcgaggc
     1981 ttggcagcgc gacggcgtgc tggcccgcgt ggactgggcc ttctcgcgcg accaggcgga
     2041 gcgcgtgcat gtgcagcacc tgctcgcgcg tgaggcgccg cgcctgcgcg agtgggtcgc
     2101 ggagggtgcg gcgatctacg tctgtggcag cctgcaaggc atggccggcg cggtcgacgc
     2161 ggtgctgcgc gagacgctcg gcgaggccga tgtcgacggg ctcatccgcg aggggcgcta
     2221 ccggcgcgac gtgtactgag ccgtccaccg atggtgacgg cgtcgtgagg cgcgcggcgc
     2281 gcggaccacg gggaaccccg taggcgcgca ggcatcgtgc gtgtcggaca tgcggagatt
     2341 gatgcgcgcc cgcgacaaac acgggggacg gcgttgactt cgaggccgtt ttccccctgc
     2401 cagagccttc tcaatgggag aggtgcgcag taccattgcc cgcgcagggt gccaacgagt
     2461 accgaaggcg tcagaccttc accagggccc ggcacatcaa caacccttga tggagagaat
     2521 cgaaatggca actgcgaaga aggccgcgcc cgccaagaag gcggcaccgg ccaagaaggc
     2581 ggcaccggcg aagaaggccg ccgctcccgc gaagaaggcg gcacctgcga agaaggccgc
     2641 gcccgcgaag aaggcagctc ctgcgaagaa ggctgccgct cccgccaaga agcgcacccc
     2701 caacgccgcc ttcatgaagg ccctgacccc cagccccgcg ctggccgcga tcgtcggcga
     2761 caagccgctg ccgcgtaccg acgtggtgaa gaaggtctgg gaatacatca agaagcacaa
     2821 cctgcaggac gctgcgcaga agcgcgtcat caatgccgac gcgaagctga aggaaatctt
     2881 cggcaaggcc aaggccgaca tgttcgagat gacccgcctg atcaacagtc acctgaagga
     2941 ttgagcgcgg aggccgcaag gcctcttctg cgttcaggac aaggcccgcc tcgcgcgggc
     3001 ttttttgcgt ccgcgcggtc ccgcaggagg gcgcgcggcg agcgtctcag agccgctcga
     3061 gcgcggcgcg cagctggcgc acgtcgatcg gcttgagcca gtaatcctgg aagcccgcgg
     3121 cgcgtgcgcg ctggcgctgg tcgtccatca cgtcggccga cagcgcgatg cagcgcagct
     3181 gggccaggcg ggcgtccgcg cgcaccagcg ccatcagttc caggccgttc atgtcgggca
     3241 ggttcatgtc catcagcacc agcgagggca tgacgcgcgg cagcatctcc atcgcctggg
     3301 cgccggtctc ggccacgtcc agcgtccagc cgggcatcag gcgaaacacc tcctccatca
     3361 gcacgatgtt gatgcggtcg tcctcgacat agaggacgtg gcggggggcg gtgggggacg
     3421 acatggcgg
//
LOCUS       DATCRO010000429         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_454454, whole genome shotgun sequence.
ACCESSION   DATCRO010000429 DATCRO010000000
VERSION     DATCRO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454454"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1092)
                     /locus_tag="VIN75_11600"
     CDS             complement(16..1092)
                     /locus_tag="VIN75_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8974856.1"
                     /translation="MHENSRRRRRHRFPTSAPAPRPRHHRILAFLVVLGLAVVCVLAL
                     GFRFGSEQMWWIEIARYVPFPAYLVPALLLVACAWPLGWRWRGLALLAPLVVATELMG
                     LAVGSPDQVGARLRFMTYNIKAYLARRTPDGYARIAWEIASQDPDVIVMQDGQSIERA
                     DELPPPMKAALKGRQVYVQDQYIVASRYPLSHCGPGNMSYSGQQDDFVHCTLSFAGKD
                     IDLFTAHFVSPRDGLNAARGKASGGLMEWKDNFRDRMAQSIQLAAAVAQARGPVIVAG
                     DLNADERSPVVRRLLATGLRDAYSSAAFGYGYTLGHDLRPGFSFLRIDHVLVSPDIGV
                     RSAAPGGREGSEHRPVVTELLVGP"
     gene            1399..2322
                     /locus_tag="VIN75_11605"
     CDS             1399..2322
                     /locus_tag="VIN75_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY8974857.1"
                     /translation="MNETTPRLFDADWSAFDPWKVTPVRHRITEHPLLQIDQLVQLSS
                     RLAKHGRIRTHSSEAYAGTAFNHAPMLYPNQRSPEETLANIASAKAWMSLLNVQTDDV
                     YRRLVDEMLDDLKPAVDRVDPGMTYRGGWIFVTSPNTVTPFHMDKEHNFIMQIKGHKR
                     LYVWEPDDVEAVSEQARDLFHANHSRDLVNWREELRSRAHVFDLEPGMGAYMPSTAPH
                     LVENGDGPSVTVSFTYYTDATRRDSRLHAMHQRLRGWGMQPAPVGTRPVMDGLLDAGF
                     GGARLLGRAVDAIRGRTTLSDRVPYAHAAIV"
     gene            2344..>5220
                     /locus_tag="VIN75_11610"
     CDS             2344..>5220
                     /locus_tag="VIN75_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8974858.1"
                     /translation="MNAVPPRDAAPATPSAQDAASRPLAALLGTRASAAGAAPRVHVL
                     ALPAGAIAADADRLPDTPWVAAWALLLARWSGQPSARWAELGDAVRVAAAVVQGTAGD
                     FLAAVDAARRAAPTAAAVDCEWACGAGAAPAGVEMLLALSGQTLELRLPADVDADLAR
                     TLAERIADVARALCASSGAPLGDIDAIGDAQRALLLHTLNAPLHPVDPQATVHGLFAR
                     VAAQRPDAPALCFCGATTTYAELDARSSRLAAQLRAHGVGPGRAVAMALERSPVAIVA
                     VLAILKAGGCYLPVDAGHPPARLAFMLQDAGATLVLCSPGHAGAFAQAEGARVLALGD
                     DGALPAPAGAPARADARDAGARDLAYLMYTSGSTGQPKGVQITHDSILRLVVDANYVR
                     LDAGTRFLHAAPLAFDASTLEIWGPLLNGGCCVLHPEHMPTPAGLQAVIRDDRVDSAW
                     LTAALFNAVVDDDPLHLKGLRQLLIGGEALSVPHVRRLLDALPEVDLINGYGPTECTT
                     FAATLHIPRDIPADARAVPIGRPINDTHAFIVGPAMELLPRGCVGKLYVGGRGVALGY
                     LNRPELTAERFVASPFAAGERLYDTGDLARWLPDGTIDFLGRADQQVKIRGFRIELGE
                     IETVIAAHPAVQACAVGAIKDAAGNTSLAAWLVAQSGTAAPDAPALRAHIARALPEFM
                     VPSAFVWMERLPITANGKLDRSALPRPANDRPELATPFRAPADAGEQRLCAAFAAVLG
                     LGRVGADDNFFELGGNSLQVIKVLARLKQDGGEVLSVAGFFRHPTPAALARQLAPADE
                     PVAPSAAEAPAPAATSGGDEPIAIIALAGRFPGARDVEQFWANLCEGRDSITRFGDGE
                     LDPSLAASLVSDPDYVKARGIVDDVEMFDPGFFGMSPREAELMDPQQRIFMELCWECL
                     ERGGHAPDGQDHGPVGIFAGMYNATYFQRHVMYRPDLVEKVGEFQ"
BASE COUNT          717 a         1935 c         1844 g          724 t
ORIGIN      
        1 tcatcacggc atttgtcacg gtccgacgag cagctccgtc accaccggcc ggtgctccga
       61 cccttcgcgt ccacccggcg ccgcggaacg cacgccgatg tccgggctga ccagcacgtg
      121 gtcgatgcgc aggaagctga atccggggcg caggtcgtgg cccagcgtgt agccgtagcc
      181 gaaggccgcg ctcgagtagg catcgcgcag gccggtggcc agcagccgcc tcaccacggg
      241 ggagcgctcg tcggcgttga ggtcgccggc gacgatcacc ggccccctgg cctgcgccac
      301 ggcggccgcc agctggatgg actgcgccat ccggtcgcgg aaattgtcct tccactccat
      361 cagcccgccc gaggccttgc cgcgggcggc gttcaggccg tcgcgcggcg agacgaagtg
      421 cgcggtgaac aggtcgatgt ccttgccggc gaagctgagc gtgcagtgca cgaagtcgtc
      481 ctgctggccg gaatacgaca tgttgcccgg gccgcagtgc gacagcgggt agcggctggc
      541 gacgatgtac tggtcctgaa cgtagacctg gcggcccttg agcgccgcct tcatcggtgg
      601 cggcagctcg tcggcgcgtt cgatcgactg gccgtcctgc atcacgatga cgtccggatc
      661 ctggctggcg atctcccagg cgatcctggc gtagccgtcg ggcgtccggc gcgcgaggta
      721 ggccttgatg ttgtaggtca tgaagcgcag ccgggcgccg acctggtcgg gcgagccgac
      781 cgccagcccc atcagctcgg tggcgacgac cagcggcgcc agcagcgcga ggccacgcca
      841 gcgccagccg agcggccagg cgcaggccac cagcagcagc gccggcacca ggtaggcggg
      901 gaacgggacg tagcgcgcga tctcgatcca ccacatctgc tcgctgccga agcgaaagcc
      961 cagcgccagg acgcacacga ccgcgaggcc gaggacgacc aggaaggcga ggatccggtg
     1021 gtggcgcggg cgtggcgccg gagcagatgt tgggaagcgg tgacggcggc gtcgacggct
     1081 gttttcgtgc acttcttctc gaattcgggg cgggaacgtg gccctatgac ttagggggaa
     1141 ggcttgagca gccgcaacgt ccgtgcattg gttccggccc ccccatccgg gcggattgcc
     1201 cgccaaaggt tacggctcgt atcatgccat tcgacagccc gacttaagca ttcgcacttt
     1261 accgccagag ccgaggagag cggccaccgc gcggcgtcga aggcccatgt cgcgtccgag
     1321 ggaggatgcg caggtcgtgg cgaccacttt tggtgtggca tggatccgcg cggatccctg
     1381 ctgttggagg acacccgcat gaatgaaacg acaccgcgcc tgttcgacgc cgactggtcg
     1441 gcgttcgatc cctggaaggt cacgccggtg cggcaccgca tcaccgagca tccgctgctc
     1501 cagatcgacc agctggtgca actgagctcg cgcctggcca agcacggccg catccgcacg
     1561 cacagcagcg aggcgtacgc cggcacggcg ttcaaccacg cgccgatgct gtatcccaac
     1621 cagcgctcgc cggaagagac gctggccaac atcgcgtcgg ccaaggcgtg gatgtcgctg
     1681 ctcaacgtgc agaccgacga cgtctatcgc cggctcgtcg acgagatgct cgacgacctg
     1741 aagcccgcgg tcgaccgggt ggacccgggc atgacgtacc gcggcggctg gatcttcgtg
     1801 acctcgccga acacggtcac gccgttccac atggacaagg aacacaactt catcatgcag
     1861 atcaaggggc acaagcgcct ctacgtctgg gagcccgacg acgtcgaggc cgtgagcgag
     1921 caggcgcgcg acctgttcca cgccaaccac agccgcgacc tcgtcaactg gcgcgaggag
     1981 ctgcgcagcc gcgcccacgt gttcgacctc gagccgggca tgggcgccta catgccgtcc
     2041 accgcgccgc acctggtcga gaacggcgac ggcccgtccg tcacggtcag cttcacgtac
     2101 tacaccgacg cgacgcgccg cgattcgcgc ttgcacgcga tgcaccagcg cctgcgcggc
     2161 tggggcatgc agcccgcgcc ggtcggcacg cgtccggtga tggatggcct gctcgatgcc
     2221 ggcttcggcg gtgcccgcct gctcggccgt gccgtggatg cgatccgcgg ccgcacgacg
     2281 ctgtccgacc gcgtgccgta cgcgcacgcg gccatcgtct gacgccgacg cgcgagccgg
     2341 atcatgaacg ccgttccgcc ccgggacgcc gcgcccgcca cgccttccgc gcaggacgcg
     2401 gcgtcgcgcc cgctggcggc gttgctgggc acgcgcgcca gcgccgcggg cgcggcgccg
     2461 cgcgtgcacg tgctggcgct gcccgccggc gcgatcgccg ccgacgctga ccgcctgccc
     2521 gacacgccct gggtcgcggc ctgggcgctg ctgctggcgc gctggagcgg ccagccgtcc
     2581 gcgcgctggg cggagctggg cgacgcggtg cgcgtcgccg cagccgtcgt ccaggggacg
     2641 gctggcgatt tcctcgccgc cgtggacgcg gcacgccgcg cggcgccgac ggctgcggcc
     2701 gtcgactgcg aatgggcctg cggtgcgggc gccgcgccgg cgggcgtcga gatgctgctg
     2761 gccctgtccg ggcagacgct ggaactgcgc ctgccggccg acgtcgatgc cgacctggcc
     2821 aggacgcttg ccgagcgcat cgcggacgtc gcgcgtgcgc tgtgcgcgtc gagcggcgcg
     2881 cccctcggcg acatcgacgc gatcggcgac gcgcagcgcg ccctgctgct gcataccctc
     2941 aacgcgccgc tgcacccggt cgacccgcag gccaccgtgc acggcctgtt cgcgcgcgtc
     3001 gcggcgcagc ggcccgatgc gccggcgctt tgcttttgcg gcgcgacgac cacgtacgcc
     3061 gaactcgatg cgcgatccag ccggctcgcg gcgcaactgc gcgcgcacgg cgtcggcccc
     3121 ggccgcgcgg tggcgatggc gctcgagcgc tcgcccgtgg cgatcgtcgc ggtgctcgcg
     3181 atcctcaagg ccggcggctg ctacctgccg gtcgacgccg gccacccgcc cgcgcgcctg
     3241 gccttcatgc tgcaggacgc cggcgcgacg ctcgtgctgt gctcgcccgg gcatgccggc
     3301 gcgttcgcgc aggccgaggg tgcgcgcgtg ctcgcgctcg gcgacgacgg cgcgctcccc
     3361 gcgcccgccg gcgcccccgc gcgcgccgac gcgcgtgacg cgggcgcccg cgacctcgcg
     3421 tacctgatgt acacgtcggg ctcgaccggc cagcccaagg gcgtgcagat cacgcacgac
     3481 tcgatcctgc gcctggtcgt cgacgccaac tacgtccgcc tggacgcagg gacgcgcttc
     3541 ctgcacgccg cgccgctcgc gttcgacgcg tcgacgctgg agatctgggg cccgctgctc
     3601 aacggcggct gctgcgtgct gcatccggag cacatgccaa cgcccgctgg cctgcaggcc
     3661 gtgatccgcg acgaccgcgt cgacagcgcg tggctcaccg ctgcgctgtt caacgccgtc
     3721 gtcgacgacg atccgctgca cctgaagggc ctgcgccagc tcctgatcgg cggcgaggcg
     3781 ctgtcggtgc cgcacgtgcg ccgcctgctg gacgcgctgc ccgaggtcga tctcatcaac
     3841 ggctacggcc cgaccgagtg caccaccttc gcggccacgc tgcacatccc ccgcgacatc
     3901 cccgccgacg cgcgcgcggt gccgatcggc cgcccgatca acgacacgca cgcgttcatc
     3961 gtcggccccg cgatggagct gctgccgcgc ggctgcgtcg gcaagctcta cgtcggcggc
     4021 cgcggcgtcg cgctcggcta cctcaatcgt ccggagctga ccgccgagcg cttcgtcgcc
     4081 agcccgttcg ccgcgggcga gcgcctgtac gacacgggcg acctcgcgcg ctggctgccc
     4141 gacggcacga tcgacttcct gggccgcgcc gaccagcagg tcaagatccg cggcttccgc
     4201 atcgagctcg gcgagatcga gacggtgatc gccgcgcatc cggccgtgca ggcctgcgcg
     4261 gtgggcgcga tcaaggatgc cgccggcaac acgtcgctgg ccgcatggct ggtggcgcag
     4321 tccggcacgg ccgcgcccga cgcgccggcg ctgcgcgcgc acatcgcgcg cgcgctgccc
     4381 gagttcatgg tgccgtccgc cttcgtctgg atggagcgcc tgcccatcac cgccaacggc
     4441 aagctcgatc gttcggcgct gccgcgcccg gccaacgacc ggcccgagct ggcgacgccg
     4501 ttccgcgcgc cggcggacgc aggcgagcag cgcctgtgcg ccgccttcgc cgcggtgctg
     4561 ggcctgggcc gcgtgggcgc cgacgacaac ttcttcgagc tgggcggcaa ctcgctgcag
     4621 gtcatcaagg tgctcgcgcg cctgaagcag gacggcggcg aggtgttgag cgtggccggc
     4681 ttcttccgcc atcccacgcc ggccgcgctt gcgcgccagc tggcgccggc ggacgagccc
     4741 gtcgcgccga gcgccgccga ggcgcccgcg cccgccgcga cgtccggcgg cgacgagccg
     4801 atcgccatca tcgcgctggc cggccgcttc cccggcgcgc gcgacgtcga gcagttctgg
     4861 gccaacctct gcgaaggccg cgacagcatc acccgcttcg gcgacggcga gctcgatccg
     4921 tcgctggcgg cctcgctggt atccgacccc gactatgtca aggcacgcgg catcgtcgac
     4981 gacgtcgaga tgttcgaccc gggcttcttc ggcatgtcgc cgcgcgaggc cgagctgatg
     5041 gatccgcagc agcgcatctt catggagctg tgctgggagt gcctggagcg cggcggccat
     5101 gcgcccgatg ggcaggacca cggcccggtc ggcatcttcg ccggcatgta caacgcgacg
     5161 tacttccagc gtcacgtgat gtaccggccg gacctggtcg agaaggtcgg cgagttccag
//
LOCUS       DATCRO010000430         6515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_533566, whole genome shotgun sequence.
ACCESSION   DATCRO010000430 DATCRO010000000
VERSION     DATCRO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533566"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1615
                     /locus_tag="VIN75_11615"
     CDS             167..1615
                     /locus_tag="VIN75_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEY8974859.1"
                     /translation="MRKTFLGVKLKTLREQRGLTQAALAQQLSLSPSYLNQLENNQRP
                     LSVPVQLRLQSALGVDLQLFSDDDEARLTSQLQAVVAQSPDGGGVSQAELRAMAQQMP
                     GLAQMLIRLHHRGRDAEDRLAALAAGLGERAGELAQAARQPHEEVRHFFYERQNHIAE
                     LDDLAERLFADVMAVEPRAQPAPGAGDAHAVAAHLETFLVEKHGIATHEFESEAGLLR
                     RFDAERRQVFVNAAAEPAQRAFQLATQLAYLEAGAAIDQLVAQGSFSGPEPRALARIG
                     LASYFAGALVLPYRRFLAAAEALRYDIDLLAQRFGVSFETVCHRLSTLQRPDARGVPF
                     FFVRVDRAGNISKRQSATDFHFSHTGGTCPLWNVYEAFAQPHRVLTQLARMPDGRTYL
                     WIARSIVRPTGGYGSPERTFAIGLGCDVRHAARLVYAKGLALADPDAATPIGAGCKVC
                     DRQGCPQRAFPALGKRLRIDENERPREPYAAS"
     gene            1664..2083
                     /locus_tag="VIN75_11620"
     CDS             1664..2083
                     /locus_tag="VIN75_11620"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8974860.1"
                     /translation="MAQAFSAGAIVYAKDIHALARFYAAVADLEVMHEVDDHIVLESE
                     AVELVIVAIPAATAARIVITTPPARRENTAFKLVFPVPSLEQARATAQANGGELNPPG
                     KEWKFQGLRVCDGCDPEGNMIQLRELPADAGLGDVED"
     gene            complement(2100..3317)
                     /locus_tag="VIN75_11625"
     CDS             complement(2100..3317)
                     /locus_tag="VIN75_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8974861.1"
                     /translation="MIAAPGSETARRPWAALLLCLALAACADAPAPPVAGEALFSDAS
                     FAPPSEPIAAADVFKLSPEMHAWLESRAAGDIKSHGEARGLLEALFMDRRLRIDYDAE
                     FTRNAAQAFEARAGNCLSLAIVTGAMAKEMGLDVRYQAISTSDHWERDGDLLELVGHV
                     NVSVALPVPKLRSWGLNTDRWTVDFLSPAEMRTLTARPITESRVAAMFMNNRAAEALA
                     HQRVDDAYWWARAGIGTDPSFASLYNTLGVTYLRKGLLARSEAALRFAVSVAPDSTQS
                     WNNLAIVLRREGLVEQAAAIDDAHPRPRAALLMAAMDDGMKANRAGDYARALELFNRA
                     LRVAGDNHQVHYQLAVTYLNLGDRRRALEHLREAEDDSLTARQRSVYASKIELLKSRQ
                     SSFRLDPDVLQVN"
     gene            3527..5005
                     /locus_tag="VIN75_11630"
     CDS             3527..5005
                     /locus_tag="VIN75_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8974862.1"
                     /translation="MTLAMSWEEWAAHDAIALAERVRRGEVTPAELAAQAAAAIARVN
                     GTLDAVVEVFDDVVADPLRDGLRPEGVFAGAPYLMKDLGPTLRGRLQEFGSMLMQGHR
                     PAQDSFLTGRIRQAGLNIIGRTTTPEFGVCSAAENRFHVTRNPWDVAFTTNGSSAGTA
                     AVVAAGVVPMAHATDGGGSIRIPAGATGNIGLKVSRGVFSLAPALSDLSGLVSIQGCH
                     SRSVRDTATFVDHCRGGAPGEFMPFWHPSTPYRELIRSNPPPLRIALSHEWGDHRATP
                     HFVAELERVGRLLEGLGHHVEWQLPPVDFRAAFDAQTTCYITNFAQVIAGHLARLGLE
                     RPPPDLLEPINIKIWEAGLHTSYSERAAMQARFNSTSRAWGAFLEDWDIVLTPVTALP
                     TPTVGAVDYLTSSTNPSVHDWFAHLWRNFAYTPLANLCGTPAISLPLAFQESGLPLGI
                     QAQTRQGDDGLLLQLAAQVERALGGQWNGGQRPRVHVSRPPS"
     gene            complement(4986..5345)
                     /locus_tag="VIN75_11635"
     CDS             complement(4986..5345)
                     /locus_tag="VIN75_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEY8974863.1"
                     /translation="MKSNPNGGLVYSTETGRMCPGCRQPIAQCTCGKPVAPATDGVVR
                     VFFETKGRGGKGVTVVRGLGLADPELAAIGKKLKAACGVGGTAKDGVVELQGDHRDRV
                     LALLPTLGFKSVKKAGG"
     gene            5418..5942
                     /locus_tag="VIN75_11640"
     CDS             5418..5942
                     /locus_tag="VIN75_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974864.1"
                     /translation="MTPARPLLRLLPAAFLLAAATSAHAAEPLLQHDVVLLEKGEVIE
                     QRVDGGSDAMAAYVKTLGEALTDTMRAHPSQLPVAGFIVVAVRPEGRTHTWFDFKPAL
                     ADATAAALAQAVATTPAMKVRSREVVFALRVSLWSNKPPKAYAPAPQEWKDATQGMHP
                     KPDVDTLVDMVWPR"
     gene            6017..>6515
                     /locus_tag="VIN75_11645"
     CDS             6017..>6515
                     /locus_tag="VIN75_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY8974865.1"
                     /translation="MTPPSPSAIAARRRAFRDLHAAGCFVIPNPWDAGSARYLASLGF
                     KALATTSSGFAWSHAQADGAMPRDAVLAHLREIVAATELPVNADFESGYGATPADVAE
                     SVRLAVATGVAGLSIEDATGDAARPIHDLDTALARLRAAREAIAAAGGDTLLVGRAEN
                     FLHGRP"
BASE COUNT          906 a         2425 c         2280 g          904 t
ORIGIN      
        1 gtcgttcagg gcgcgcggcg atgccaagca tccacgcgcg agtgtgcgcc ttccatcata
       61 tttgcaatct tcgcagtcgg gtattgcttt tcatctccag ggaaagtagc gaagatttgc
      121 gaacagctcc gccatttatg caaagtttgc gaacttcgaa tttgccatgc gcaagacctt
      181 cctcggcgtg aagctcaaga ccctgcgcga gcagcgcggg ctgacgcagg ccgccctcgc
      241 ccagcagctg tcgctgtcgc ccagctacct gaaccagctg gagaacaacc agcggccact
      301 gagcgtgccc gtgcaactgc gcctgcagtc ggccctgggc gtcgacctgc agctgttctc
      361 ggacgacgac gaggcgcgcc tgacctcgca actgcaggcc gtggtggcgc aaagcccgga
      421 cggcggcggc gtctcgcagg ccgagctgcg cgcgatggcg cagcagatgc cgggactcgc
      481 gcagatgctg atccgcctgc accaccgcgg ccgcgatgcc gaggaccggc tggcggcgct
      541 ggccgccggc ctgggcgagc gcgccggcga gctggcccag gcggcgcgcc agccgcacga
      601 ggaggtgcgc cacttcttct acgagcgcca gaaccacatc gccgaactcg acgacctggc
      661 cgagcgcctg ttcgccgacg tgatggccgt cgagccgcgc gcccagcccg cgcccggcgc
      721 cggcgacgcg cacgcggtcg ccgcgcacct ggagaccttc ctggtcgaga agcacggcat
      781 cgcgacccac gagttcgagt ccgaggcagg cctgctgcgc cgcttcgacg ccgaacgccg
      841 gcaggtgttc gtcaacgccg ccgccgagcc cgcgcagcgc gcgttccagc tcgccacgca
      901 gctggcctac ctcgaggccg gcgccgcgat cgaccagctg gtcgcgcaag gcagcttcag
      961 cggccccgag ccgcgcgcgc tggcccgcat cggcctggcg agctacttcg ccggcgcgct
     1021 ggtgctgccc taccgccgct tcctcgccgc ggccgaggcg ctgcgctacg acatcgacct
     1081 gctcgcgcag cgcttcggcg tcagcttcga gacggtgtgc caccggctgt cgacgctgca
     1141 gcggccggac gcgcgcggcg tgccgttctt cttcgtgcgc gtcgaccgcg cgggcaacat
     1201 ctccaagcgc cagtcggcca ccgacttcca cttctcgcac acgggcggca cctgcccgct
     1261 gtggaacgtc tacgaggcct tcgcgcagcc gcacagggtg ctgacgcagc tcgcgcgcat
     1321 gcccgacggc cgcacctacc tgtggatcgc gcgcagcatc gtgcgcccca ccggcggcta
     1381 cggctcgccc gagcgcacct tcgcgatcgg gctgggctgc gacgtgcgcc acgcggcgcg
     1441 cctggtctat gccaagggcc tggcgctggc cgaccccgac gccgccacgc ccatcggcgc
     1501 cggctgcaag gtgtgcgacc gccagggctg cccgcagcgc gcgttcccgg cactgggcaa
     1561 gcggctgcgc atcgacgaga acgagcggcc gcgcgagccg tacgcggcga gctgacggcc
     1621 gcgcttggca tactgaggca cgacaacatc gcaggagcca cccgtggccc aagccttctc
     1681 cgccggcgcc atcgtctacg ccaaggacat ccacgccctc gcccgcttct acgccgcggt
     1741 ggccgacctc gaggtcatgc acgaggtcga cgaccacatc gtgctcgagt cggaggccgt
     1801 ggagctggtc atcgtcgcga tccccgccgc cacggccgcg cgcatcgtca tcacgacgcc
     1861 cccggcgcgg cgcgagaaca ccgccttcaa gctcgtcttt ccggtgccca gcctcgagca
     1921 ggcgcgcgcg acggcccagg ccaacggcgg cgagctgaac ccgcccggca aggaatggaa
     1981 gttccagggc ctgcgcgtgt gcgacggctg cgatcccgag ggcaacatga tccagctgcg
     2041 cgagctgccc gcggacgcgg ggctcgggga cgtcgaggac tgaccgccgg cgacggccgt
     2101 cagttgacct gcaggacgtc cggatccagc cggaagctgc tctgcctgga cttgagcagt
     2161 tcgatcttcg acgcgtagac cgagcgctgg cgggcggtca ggctgtcgtc ctccgcctcg
     2221 cgcaggtgct ccagggcgcg gcgccggtcg ccgaggttca ggtacgtgac ggcgagctgg
     2281 tagtgcacct ggtggttgtc gccggccacg cgcagcgcgc ggttgaacag ctcgagcgcg
     2341 cgcgcatagt cgcccgcccg gttggccttc atcccgtcgt ccatggccgc catcagcagc
     2401 gcggcgcgcg gacgcggatg ggcgtcgtcg atcgccgcgg cctgttcgac caggccttcc
     2461 cggcgcagga cgatcgccag gttgttccac gactgggtgc tgtccggcgc gaccgacacc
     2521 gcgaagcgca gcgcggcttc cgagcgggcg agcaggccct tgcgcaggta tgtcacgccc
     2581 agcgtgttgt acagcgaggc gaacgacggg tccgtgccga tgccggcgcg ggcccaccag
     2641 taggcgtcgt ccacgcgctg gtgcgccagg gcctcggcgg cccggttgtt catgaacatc
     2701 gccgcgacgc gcgactccgt gatcgggcgc gccgtcaggg tcctcatctc ggccggggac
     2761 aggaagtcga ccgtccagcg atccgtgttc aggccccagc tgcgcagctt ggggacgggc
     2821 agcgccaccg agacgttcac gtgcccgacg agctccagca ggtcgccgtc gcgctcccag
     2881 tggtcgctgg tgctgatcgc ctggtagcgc acgtccagcc ccatctcctt ggccatcgcg
     2941 ccggtcacga tggccagcga caggcagttg ccggcccgtg cctcgaacgc ctgcgccgcg
     3001 ttgcgcgtga actcggcgtc gtagtcgatc ctgagccgcc ggtccatgaa caaggcctcg
     3061 agcaggcctc gcgcctcgcc gtgcgacttg atgtccccgg ccgcccggga ttccagccag
     3121 gcgtgcatct cggggctcag cttgaagacg tcggcggcgg cgatgggctc gctgggcggc
     3181 gcgaacgacg cgtcggagaa cagcgcctcg ccggcgacgg gcggggcggg cgcgtccgcg
     3241 cacgcggcca gcgccaggca aaggagcagc gccgcccagg ggcgtcgtgc cgtctcggaa
     3301 cccggtgccg cgatcatggg ccgcatcctc gtcgtttccg gcgcgccgcg ggtacgggcc
     3361 gccggatcct gcccccgagt gtgctgcggc ggatcgcgct tggcaaccgt gcagaaccgg
     3421 gtgggcgagc gatgccgcga cgccgccgca agggttacga tggcccgcgc caccggcatc
     3481 ggcaggtctc gcggcgagac cggtcaccat cacccgaagg acagccatga cgctcgcgat
     3541 gagctgggag gagtgggcgg cgcacgacgc catcgcgctg gccgagcgcg tgcgccgggg
     3601 cgaggtcacg ccggccgaac tcgcggcgca ggcggccgcg gcgatcgcgc gcgtgaacgg
     3661 cacgctggac gcggtggtgg aagtcttcga cgacgtcgtg gccgacccgc tgcgcgacgg
     3721 cctgcggccc gagggcgtgt tcgccggcgc gccgtacctg atgaaggacc tcgggccgac
     3781 cctgcgcggg cgtctgcagg aattcggctc gatgctgatg cagggccatc ggcccgcgca
     3841 ggacagcttc ctgaccggac gcatccgcca ggccggcctg aacatcatcg gccgcacgac
     3901 cacgccggag ttcggcgtct gcagcgcggc cgagaaccgc ttccatgtca cgcgcaatcc
     3961 gtgggacgtc gcgttcacca ccaacggctc gtccgccggg accgccgccg tcgtggctgc
     4021 gggcgtcgtg ccgatggcgc atgcgaccga cggcggcggc tccatccgga tcccggcggg
     4081 cgcgaccggc aacatcggcc tgaaggtgtc gcgcggcgtg ttctcgctgg cgccggcgct
     4141 gtcggacctg tcgggcctgg tgtcgatcca gggctgccac agccgctcgg tgcgcgacac
     4201 cgccaccttc gtcgaccact gccgcggcgg cgcgccgggc gagttcatgc cgttctggca
     4261 cccttcgacg ccgtaccgcg aactcatccg cagcaacccg ccgccgctgc gcatcgcgct
     4321 gtcgcacgag tggggcgacc atcgcgccac gccgcacttc gtggccgagc tcgagcgcgt
     4381 cggacgcctg ctcgaagggc tgggccacca cgtcgagtgg cagctgccgc cggtggattt
     4441 ccgcgccgcc ttcgacgcgc agaccacctg ctacatcacc aacttcgcgc aggtcatcgc
     4501 gggccacctc gcgcgcctgg gactggagcg gccgccgcca gacctcctcg agccgatcaa
     4561 catcaagatc tgggaggccg ggctgcacac gtcgtacagc gagcgcgccg cgatgcaggc
     4621 ccggttcaac agcacgtcaa gggcatgggg cgcgttcctc gaggactggg acatcgtcct
     4681 gaccccggtc accgcgctgc ccacgccgac ggtgggcgcg gtcgactacc tcacgagcag
     4741 caccaacccg tcggtgcacg actggttcgc ccacctgtgg cgcaacttcg cctacacgcc
     4801 gctcgccaac ctgtgcggca cgccggccat ctcgctgccc ctcgccttcc aggagagcgg
     4861 cctgccgctg ggcatccagg cgcagacgcg acaaggcgac gacggcctgc tgctgcaact
     4921 ggccgcgcag gtcgagcggg cgctgggcgg ccagtggaac ggcggccagc ggccgcgggt
     4981 gcacgtcagc cgcccgcctt cttgacgctc ttgaacccga gcgtcggcag cagcgccagc
     5041 acgcggtcgc ggtggtcgcc ctgcagctcg acgacgccgt ccttggccgt gccgccgacg
     5101 ccgcacgcgg ccttgagctt cttgccgatc gccgcgagct cggggtcggc caggcccagg
     5161 ccgcgcacca cggtcacgcc cttgccgccg cggcccttgg tctcgaagaa gacgcgcacg
     5221 acgccgtcgg tcgccggggc gacgggcttg ccgcaggtgc actgcgcgat cggctggcgg
     5281 cagccggggc acatgcggcc cgtctcggtc gagtagacca ggccgccgtt cggattcgat
     5341 ttcatgaccg tattggaacc gatccgcgcg ccggcgctcc cgccggctcg gccggcgcct
     5401 gatgtgaaaa tcgcgccatg actcccgccc gtcccctgct ccgcctgctt cctgccgcct
     5461 tcctgctcgc cgcggcgacc tctgcccatg ccgccgagcc gctgctgcag cacgacgtcg
     5521 tgctgctgga gaagggcgaa gtcatcgagc agcgcgtgga cggcggctcg gacgcgatgg
     5581 cggcctacgt caagacgctc ggcgaggcgc tcaccgacac gatgcgcgcg catccgtcgc
     5641 agttgcctgt ggcgggcttc atcgtcgtgg ccgtgcggcc cgagggcagg acgcacacgt
     5701 ggttcgactt caagcccgcg ctggccgacg ccacggcggc cgcactcgcg caggcggtgg
     5761 cgaccactcc ggccatgaag gtcaggagcc gcgaggtcgt cttcgcgctg cgcgtgagcc
     5821 tgtggagcaa caagccgccc aaggcctatg cgccggcgcc gcaggagtgg aaggacgcca
     5881 cccagggcat gcatcccaag cccgacgtcg acacgctggt ggacatggtg tggccgcggt
     5941 gattcgctga cggacgaagc gtcgcgcggg cggacgcgtt aacctgcggc ttcagacttc
     6001 cgctggagcc ctgcgcatga cgccgccctc gccttccgcc atcgccgccc gccgccgcgc
     6061 cttccgcgac ctgcacgccg ccggctgctt cgtcatcccg aacccgtggg acgccggcag
     6121 cgcgcgctac ctcgcgtcgc tgggcttcaa ggcgctggcc accacgagct cgggcttcgc
     6181 gtggtcgcat gcgcaggcgg acggcgcgat gccgcgcgac gccgtgctgg cgcacctgcg
     6241 cgagatcgtc gcggccaccg agctaccggt gaacgccgac ttcgagagcg gctacggcgc
     6301 cacgcccgcc gacgtggccg agagcgtgcg gctggccgtg gcgaccggtg tcgcgggcct
     6361 gtcgatcgag gacgccacgg gcgatgccgc gcgcccgatc catgacctgg acacggccct
     6421 cgcgcgcctg cgcgccgcgc gcgaggcgat cgccgccgcc ggcggcgaca cgctgctcgt
     6481 gggccgcgcc gagaacttcc tgcacggccg gcccg
//
LOCUS       DATCRO010000431         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_409570, whole genome shotgun sequence.
ACCESSION   DATCRO010000431 DATCRO010000000
VERSION     DATCRO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409570"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VIN75_11650"
     CDS             complement(<1..622)
                     /locus_tag="VIN75_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8974866.1"
                     /translation="MLSIAAVERETGLGKDTLRAWERRYGFPMPERDAAGVRGYPRAL
                     VDRLALIRRALLAGERPGRLLTLLPEQLDEVLSDIASRVPALVRGGPHDGKPVLDVAE
                     GIVALRTGGADALRHWLTLGLARTGLAAFVVDGIAPLTAAVGDAWLDGRIAVYEEHLY
                     SEAVQAVLRSALLPFQAGLEARGPRILLTTLPGEAHRLGLLMAEALM"
     gene            1174..2538
                     /locus_tag="VIN75_11655"
     CDS             1174..2538
                     /locus_tag="VIN75_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8974867.1"
                     /translation="MSRPRIAVVGSGIAGLGAAHRLAPHCDVTVFEADDRLGGHANTV
                     DVTLPGRDGPVTHGVDTGFLVFNERTYPRLCALFDELGVEVAKSEMTFSVQAPRPGGG
                     RLEWSGTSLNTVFADRGNLLRPRFHGMLWDLLRFNRLATALARRGVDAELAQPLDAFL
                     AEHRFGTAFVDDYLLPMIGCIWSCSTVQMLRFPVGTLIRFCHNHGLLQVRERPQWFTV
                     TGGSRRYVDKLATRIGRVRLSTPVLGIARDASRAAGDRVGVRTRTGTEWFDELVLAAH
                     APQALALLDDASAEERRTLGAIGYQDNEAVLHTDERLLPRRRAAWSAWNYQRDVVSGS
                     NSQNVCLHYLINQLQPLPWRQSVIVSLNPLHAPRPETVLRRITYAHPVFDNAAIAAQG
                     RLAELQGRSHVWFCGAWTGYGFHEDGLKSGQEVAGRVLARRSAAPVAAIDPDVAVEPL
                     REAA"
     gene            2535..3410
                     /locus_tag="VIN75_11660"
     CDS             2535..3410
                     /locus_tag="VIN75_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY8974868.1"
                     /translation="MNGPRARIGFGEVRHQRLAPVVHAFAYPTCFLLLPMRALRAAAD
                     PALARNRRALFGFRDADHGDGRDDSLAWLDGLLASEGITDADGQVWLHCIPRMFGFAF
                     KPVSFWYAHRADGALAAIVVEVNNTFGERHCYLLAGDGLAFGRTLQARKVFHVSPFCE
                     TRGDYHFRFMRTDLDSDQGQGRTAVRVELHVDGAPLLLTSVSGKLEPLTRGAVRLALW
                     TLPAMTALIVARIHWHALRLWLGRLAFVPRPAPPRRAVSGKDGLADPAPTLREAGEAR
                     VSPASDDATSIQNPA"
     gene            3455..4642
                     /locus_tag="VIN75_11665"
     CDS             3455..4642
                     /locus_tag="VIN75_11665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY8974869.1"
                     /translation="MARAPAAARAALALVRRLQAGTLVVRTPDGSTHQAAGATQPELH
                     AVLDVHDWSTFTATLRSGDIGFAETFMAGAWTTPQLADLLRLILANREVIDKAIYGSW
                     WGSLLHRVQHALNRNTRRGSKRNIQAHYDLGNDFYVLWLDSTMTYSSAWFDGDRTQPL
                     ERAQTAKLRRAIAATGVRPGERLLEIGCGWGAVAEAAAREGIDIVGLTLSKEQLAWAQ
                     ARLERAGVAAHADLRLQDYRDVPDGPFDAIVSVEMFEAVGREYWDAYFQTVRRQLKPG
                     GKACIQAIVIRDDLFERYARSSDFIQRHVFPGGMLPSTALFEQHARAAGLRVVERFAF
                     GPDYAETLRRWRHAFLARLDEVRAQGFDERFVRLWEFYLAYCEAAFDAGNTDVIQFTL
                     QGD"
     gene            4893..>5075
                     /gene="raiA"
                     /locus_tag="VIN75_11670"
     CDS             4893..>5075
                     /gene="raiA"
                     /locus_tag="VIN75_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY8974870.1"
                     /translation="MNLTISGHHLEVTPALREYVLTKLDRITRHFDGVVDVNVLLTVE
                     NLKEKERRQKAEVTLHV"
BASE COUNT          744 a         1850 c         1747 g          734 t
ORIGIN      
        1 tcatcagcgc ctcggccatc aggaggccca gcctgtgcgc ctcgccgggc agcgtggtca
       61 gcaggatgcg cgggccgcgc gcctccagcc cggcctggaa cggcagcagc gcgctgcgca
      121 gcaccgcctg cacggcctcg ctgtacaggt gctcctcgta gacggcaatg cggccgtcga
      181 gccaggcatc gcccacggcc gccgtcagcg gcgcgatgcc gtcgaccacg aaggcggcca
      241 ggcccgtgcg cgcgaggccc agcgtgagcc agtgccgcaa ggcgtcggca ccgccggtcc
      301 gcagcgcgac gatgccctcg gcgacgtcca gcaccggctt gccatcgtgc gggccgccgc
      361 gcaccagcgc cggcacgcgc gaggcgatgt ccgacagcac ctcgtcgagc tgttcgggca
      421 gcagcgtgag cagccggccg ggccgctcgc ccgccagcag cgcccgccgg atcagggcca
      481 ggcgatcgac cagcgcgcgc gggtagccgc gcacgccggc ggcgtcgcgc tcgggcatcg
      541 ggaaaccgta gcggcgctcc caggcgcgca gcgtgtcctt gcccaggccc gtctcgcgct
      601 cgaccgccgc gatcgacaac aacggcgcgt cggtgcgacg ccgcgagtgc ccggcaggtg
      661 aatccgaaga gggatgagct gacgtatcca tggcatgcaa gccgtgaaat ccacattcgt
      721 gcaaacagac ctggccgacg cgccggcctg gatccattgg atcgcagaag cgccaatccg
      781 tcctggacaa tttgtcacag cgacggcaca gtatccccac aactcggcca tgaagcccat
      841 gtttgtccag gacaaatact tgtctcacca caccgacacg gcccctcctg cggggcacaa
      901 cccccctcga atcaagggga atgacgcggg gggccacgga acttcgagcg caaaatcccg
      961 tcctatcgac tgtgcgaggg cgagggtgcc gtcgcaccgc cgcgaacccc gcggcgtcat
     1021 ggccggttta ctcgctgcgc agcttcgtcg gttcgccctc gaaacgtccc gagcgcttct
     1081 cctcctccct ccctcccttg ttcgctcagg ggcgctgcgc agcgacattt atgcatccgc
     1141 acccatcccc gggtgccgcg cgggagcccg gcggtgagcc gcccacgcat cgcggtcgtc
     1201 ggttcgggca ttgccgggct gggcgccgcc catcgtctcg cgccgcactg cgacgtcacc
     1261 gtcttcgagg cggacgaccg cctgggcggc cacgccaaca ccgtcgacgt cacgctgccg
     1321 ggccgcgacg gcccggtcac gcacggcgtg gacaccggct ttctcgtgtt caacgaacgc
     1381 acctacccgc gcctgtgcgc gctgttcgac gaactgggcg tcgaggtggc caagtcggag
     1441 atgacgttct ccgtacaggc gcctcgcccc ggcggcggcc ggctcgagtg gagtggcacc
     1501 agcctgaaca ccgtcttcgc cgaccgcggc aacctgctgc gcccgcgctt ccacgggatg
     1561 ctgtgggacc tgctgcgctt caaccgcctg gccaccgcgc tggccaggcg tggcgtcgac
     1621 gccgagctgg cgcagccgct ggacgcattt ctcgccgagc accgcttcgg caccgccttc
     1681 gtcgacgact acctgctgcc gatgatcggc tgcatctggt cgtgctccac ggtgcagatg
     1741 ctgcgcttcc ccgtgggcac gctgatccgc ttctgccaca accacggcct gctgcaggtg
     1801 cgcgagcggc cgcagtggtt caccgtcacg ggcggctcgc gccgctacgt cgacaagctg
     1861 gccacgcgca tcggccgcgt gcggctgtcg acaccggtgc tgggcatcgc gcgcgacgcg
     1921 tcgcgcgcgg cgggcgaccg ggtgggcgtg cgcacgcgca cgggcaccga gtggttcgac
     1981 gaactcgtgc tggccgcgca cgcgccgcag gcgctggcgc tgctggacga cgcgagcgcg
     2041 gaagagcgcc gcacgctcgg cgcgatcggc taccaggaca acgaggccgt gctgcacacc
     2101 gacgagcggc tgctgccgcg gcgccgcgcc gcatggtcgg cgtggaacta ccagcgcgac
     2161 gtggtctcgg gcagcaacag ccagaacgta tgcctgcact acctgatcaa ccagttgcag
     2221 cccctgccgt ggcggcagtc ggtgatcgtc tcgctgaacc ccctgcacgc gccgcgtccc
     2281 gagacggtgc tgcgccgcat cacctatgcg caccccgtgt tcgacaacgc cgccatcgcc
     2341 gcgcagggcc gcctggccga gctgcagggc cgctcgcacg tgtggttctg cggcgcatgg
     2401 acaggctacg gcttccatga ggacggactg aagtcgggcc aggaggtggc cggtcgcgtc
     2461 ctcgcgcgcc ggtccgccgc tccggtggcc gccatcgatc cggacgtggc cgtcgagccg
     2521 ctgcgcgagg ccgcatgaac gggccgcgcg cgcgcatcgg cttcggcgag gtgcgtcacc
     2581 agcgcctcgc gccggtcgtg cacgcgttcg cgtatccgac ctgctttctc ctgctgccga
     2641 tgcgcgcgct gcgcgccgcg gccgaccccg cgctcgcgcg caaccggcgc gcgctgttcg
     2701 gtttccgtga cgccgaccac ggcgacggcc gcgacgacag cctcgcctgg ctcgacggcc
     2761 tgctcgcgtc cgaaggcatc acggacgccg acgggcaggt gtggctgcac tgcatcccgc
     2821 gcatgttcgg cttcgcgttc aagccggtca gcttctggta cgcgcaccgg gccgacggcg
     2881 cgctcgcggc catcgtcgtc gaggtcaaca acaccttcgg cgagcgccat tgctacctgc
     2941 tggcgggcga cgggctcgcc ttcgggcgca cgctgcaggc gcgcaaggtg ttccacgtgt
     3001 cgcccttctg cgagacgcgc ggcgactacc acttccgttt catgcgcacc gacctcgatt
     3061 cggatcaggg ccagggacgc accgcggtgc gcgtcgagct gcacgtggac ggcgcaccgc
     3121 tgctgctgac gagcgtgtcc ggaaagctcg agcccctgac gcgcggggcc gtgcgcttgg
     3181 cgctgtggac gctgcccgcg atgaccgcgc tgatcgtggc gcgcatccac tggcacgcgc
     3241 tgcggctgtg gctcgggcgg ctggcgttcg tgcccaggcc ggcaccgccg cggcgcgcgg
     3301 tcagcggcaa ggacggcctc gccgaccccg cgccgacctt gcgcgaggct ggcgaggcgc
     3361 gggtttcacc ggcaagcgac gacgcgacgt cgatccagaa tccggcatga cctccggaac
     3421 gctcccgccg tcatccgagg cggccggccg cgccatggct cgcgcgcccg ccgccgcccg
     3481 cgccgcgttg gccctggtgc ggcgcctgca ggccggcacg ctcgtcgtgc gcacgcccga
     3541 cggcagcacc caccaggcgg ccggcgcgac gcagcccgag ctgcacgcgg tgctcgacgt
     3601 gcacgactgg tccaccttca ccgccacgct gcgcagcggc gacatcggct ttgccgagac
     3661 cttcatggcc ggcgcctgga cgacgccgca gctggccgac ctgctgcggc tcatcctggc
     3721 caaccgcgag gtcatcgaca aggcgatcta cggcagctgg tggggctcgc tgctgcatcg
     3781 cgtgcagcac gcgctcaacc gcaacacgcg ccgcggcagc aagcgcaaca tccaggccca
     3841 ctacgacctc ggcaacgatt tctatgtgct gtggctcgac tcgacgatga cgtactcgag
     3901 cgcgtggttc gacggcgacc gcacgcagcc gctcgaacgc gcgcagaccg ccaagctgcg
     3961 gcgtgcgatc gcggcgaccg gcgtgcggcc gggcgagcgc ctgctggaga tcggctgcgg
     4021 ctggggcgcg gtggccgagg ccgcggcgcg cgaaggcatc gacatcgtcg gactcacgct
     4081 gtcgaaggag cagctcgcgt gggcgcaggc gcgcctggag cgcgcgggcg tggccgcgca
     4141 cgccgacctg cggctgcagg actaccgcga cgtgcccgac gggccgttcg acgcgatcgt
     4201 gtcggtggag atgttcgagg ccgtcggccg cgagtattgg gacgcgtatt tccagaccgt
     4261 gcggcgccag ctcaagccgg gcggcaaggc gtgcatccag gcgatcgtga tccgcgacga
     4321 cctgttcgag cgctatgcgc ggtcgtcgga tttcatccag cgccacgtgt tccccggcgg
     4381 catgctgccg agcaccgcgc tgttcgagca gcatgcgcgc gccgcgggcc tgcgcgtggt
     4441 cgagcgcttc gcgttcggac ccgactacgc cgagacgctg cgccgctggc gccatgcgtt
     4501 cctcgcgcgg ctggacgagg tgcgcgcgca gggtttcgac gagcgcttcg tccggctgtg
     4561 ggagttctac ctcgcgtact gcgaagcggc cttcgacgcg ggcaacaccg acgtcatcca
     4621 gttcacgctg cagggcgatt gagggcgatc aggacgcgct cgaccggcgg gagccgggag
     4681 cctggcagcc cctggacaag cacgtcacgg tgccgtcatc aacgtgaata tagcccggat
     4741 ttccgcattt ggggaggctg gtcaaaccgg tgggccgggc gcatcatgga ctcgtgctgc
     4801 tgcctcgacg tcgactggaa aggcaggcac ggtcgacccg gatctcccca tccgggccat
     4861 gtcccgcaac tcacgcttga aaggaggcac tgatgaatct gacgatcagc ggccatcatc
     4921 tggaagtgac acctgccctg cgcgaatacg tcctgaccaa gctggacagg atcacgcgcc
     4981 acttcgacgg cgtcgtcgac gtgaacgtgt tgctcaccgt ggaaaatctc aaggaaaagg
     5041 aacgtcgaca aaaagccgaa gtcactctcc acgtg
//
LOCUS       DATCRO010000432         8247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1016843, whole genome shotgun sequence.
ACCESSION   DATCRO010000432 DATCRO010000000
VERSION     DATCRO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016843"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VIN75_11675"
     CDS             complement(<1..894)
                     /locus_tag="VIN75_11675"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY8974871.1"
                     /translation="MTPAPDREPPSTPAPHGALHAGPPFWRRALERIQRWHPALRLTL
                     VATVLWLVGAWHIAVSPLGPLTRLELALDDVRQSTALAPVTQARQDIVIVDVDDASLR
                     ELGRWPWPRDRLARLVETLFDQDRAAALGIDLVLAEPDDQARGVRDALSALTRLVPDD
                     AALRAGVTRWRATLEAQADHDARLARALAGHPVTLAYHFNHQAGPAAQGAARELPAPI
                     TEADVLPASVLAMTPWQGVEAPVPELLRAASATGFINALPDADGELRSTLLVTGYGGA
                     VYESFALSLWRQWRHVSALRPV"
     gene            complement(920..1582)
                     /locus_tag="VIN75_11680"
     CDS             complement(920..1582)
                     /locus_tag="VIN75_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8974872.1"
                     /translation="MGKLVVSLDNVVIKEFQITKERTTLGRRPYNDIVIDNLAVSGEH
                     AVLTAAQSDVYIEDLNSTNGTYINGKAVKKQLLSNNDVVEVGKYRIKFLAEEAEDYEK
                     TMILRPGAFGRAPGAVPPGAPGSAAAATPAAAGPASIKVLNGGAAGREVLLTKVVTTV
                     GKPGVQVASVTKRPGGYVLSHVEGSRRPNVNGQPVGEEAISLKHGDIIDLAGTQMQFV
                     QN"
     gene            complement(1681..2466)
                     /locus_tag="VIN75_11685"
     CDS             complement(1681..2466)
                     /locus_tag="VIN75_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY8974873.1"
                     /translation="MILEFFSVTDTGRARRNNEDSVAVDEAGRIAVLADGMGGYNAGE
                     VASGMASERVKGELGQRLKGLGATPTDSEMKSALLDAVDSANREVYDAAMSHPEYAGM
                     GTTLVVAVYRGERLWLGHIGDSRGYRFRNGHLEQLTRDHSLLQEQIDAGLITAEQAAY
                     SMHKNLVTRAVGVDELVDLEIHDYPVEPGDLLLMCSDGLSDMLSDEQIEQLLRTNDSL
                     PAAGAMLVQAANAAGGRDNIAVILARAKAGTQGFGKSWWSFWR"
     gene            complement(2506..3531)
                     /locus_tag="VIN75_11690"
     CDS             complement(2506..3531)
                     /locus_tag="VIN75_11690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8974874.1"
                     /translation="MFGLAPGAAAAPAMQHLQVPQFGPATDMPKVTDYALQHVIARSD
                     RATIYQATDRASGQLIALKTVRIGSASGTDRGLWRERFLREAEAAARLKHDYIVTVHA
                     GGVQGEGDATTGWLAMEWVHGTDMSRYASANRLLPEGVVVGICQRVALALDLAHRSGI
                     VHRDIKPSNVLFDPTTGVVKVTDFGSARVADMAATRSGVMLGTPAYMAPEQLAGADAT
                     AQCDLYALGVLLFELLIGRRPFESDSMGDLLARIAHQDAPRVRELRPELPPLLDDVVA
                     RLLAKSPGLRQESARQVVLELRLARQQCAAAEPGSAAADWPDTVSGDDPLHELTASSS
                     FQGTIAS"
     gene            complement(3706..4389)
                     /locus_tag="VIN75_11695"
     CDS             complement(3706..4389)
                     /locus_tag="VIN75_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8974875.1"
                     /translation="MDLLLTPAFWAALGSIILANVLLSGDNAVVIAMAARGLPARQQK
                     KAIVFGSGAAIVMRIVLTLIAVKMLGLPWLKLVGGIALLWIGANLMSEEEEQHDQEAR
                     PIGIGAAVRTILVADLVMSLDNVLAVAAAAQGNTPLLVIGLAVSIPLIVFGSTIILKV
                     MERFPAIIVAGAALLGWLAGEMMLTDPAIPPRLGEPSANAVRIAGIVGAILVVIAGKL
                     LQRRAKARA"
     gene            complement(4548..5441)
                     /gene="sucD"
                     /locus_tag="VIN75_11700"
     CDS             complement(4548..5441)
                     /gene="sucD"
                     /locus_tag="VIN75_11700"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8974876.1"
                     /translation="MSIYINKDTKVITQGITGKTGQFHTLGCQAYANGKNAFVAGVNP
                     KKAGEKFSDIPIFATVAEAKQQTGADVSVIYVPPAGAAAAIWEAVEADLDLVVCITEG
                     IPVRDMLMVRNKMKAKEAAGGKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMKAFNDDPETDAVIMIGE
                     IGGPDEADAARWCKGNMKKPIVGFIAGVTAPPGKRMGHAGALISGGADTADAKLAIME
                     ECGFKVTRNPSEMGKLLKALL"
     gene            complement(5471..6631)
                     /gene="sucC"
                     /locus_tag="VIN75_11705"
     CDS             complement(5471..6631)
                     /gene="sucC"
                     /locus_tag="VIN75_11705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8974877.1"
                     /translation="MKIHEYQGKELLRQFGVPVPRGYPAFTVQEAVEAAQKLGGGVWV
                     VKAQIHAGGRGKGGGVKLARSMDDLKKLSGEILGMQLKTHQTGPEGQKVRRLYIEEGA
                     DIKKEYYVSLVTDRATQKVAFIASSEGGMDIEEVAHSTPEKIITEVIDPLTGLKADQA
                     KKIAAAIGLPEGSQAQAVDLFQKLYQCYMDTDASLVEINPLNCDSKGNLIALDAKFNF
                     DANALFRHPEIVAYRDLDEEDPAEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATAEKVTEAFKIMLKNPHVKGILVNIFGGIMKCDTIAEGVIT
                     ACKAVNLQVPLVVRMKGTNEELGKKMLAESGLPIIAADTMAEAATKIVAAVK"
     gene            complement(6931..7761)
                     /locus_tag="VIN75_11710"
     CDS             complement(6931..7761)
                     /locus_tag="VIN75_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY8974878.1"
                     /translation="MQAIGFLSRREHSRQELRAKLLESLRKRAREDAALEAAAEEVRQ
                     AALAQPSNGIDDAFAPRAAEPSPTPARTQASRPGARPQSQALDTLTFATGAALPAHLR
                     ACLEAGAGSSADPAASQRRPGRAVPANPSGDVLEDAGRSDHASSEPVVPRDCPDDADL
                     ERTDPETAVDRLLDWLVVNKYLSDARFVESRVNARSRKQGTLRIKLELSRHGLALEPE
                     QATALRETEFARARELWQRKFGEVAADPRQRARQARFLAARGFAADVVRRVVGGLDED
                     "
     gene            complement(7910..>8247)
                     /gene="recA"
                     /locus_tag="VIN75_11715"
     CDS             complement(7910..>8247)
                     /gene="recA"
                     /locus_tag="VIN75_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202806.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8974879.1"
                     /translation="GNETKVKVVKNKVSPPFKEAEFDILYGEGISREGEIIDLGVNAK
                     VIEKSGAWYAYNGEKIGQGKDNCREFLKENPALAIEIENKVRESLGVPLVPVVEAPAA
                     KKGKAAAAE"
BASE COUNT         1153 a         2748 c         2956 g         1390 t
ORIGIN      
        1 caccggccgc agcgcgctca cgtgacgcca ttggcgccac agcgacagcg cgaacgattc
       61 gtagaccgcg ccgccgtagc ccgtcaccag cagcgtcgag cgcagctcgc cgtcggcgtc
      121 cggcagcgcg ttgatgaagc cggtggccga cgccgcgcgc agcagctcgg gcaccggcgc
      181 ctcgacgccc tgccacggcg tcatcgccag cacgctggcg ggcagcacgt cggcctcggt
      241 gatcggggcc ggcagctcgc gggcggcgcc ttgcgcggca ggcccggcct ggtggttgaa
      301 gtggtatgcg agcgtgaccg ggtgccccgc gagcgcgcgc gcgaggcggg cgtcgtggtc
      361 ggcctgcgcc tccagcgtcg cgcgccagcg cgtgacgccc gcgcgcaggg ccgcgtcgtc
      421 gggcacgagt cgcgtcagcg cggacagcgc gtcgcgcacg ccgcgcgcct ggtcgtcggg
      481 ctcggccagc acgaggtcga tgcccagcgc agcggcgcga tcctggtcga acagcgtctc
      541 gaccagccgg gccagccggt cgcgcggcca gggccagcga ccgagctcgc gcaggctggc
      601 gtcgtcgacg tcgacgatga cgatgtcctg ccgcgcctgc gtcaccggcg cgagggccgt
      661 ggactggcgc acgtcgtcga gcgccagctc caggcgcgtg agcggcccga gcggcgagac
      721 ggcgatgtgc cacgcgccga ccagccacag gacggtggcc accagcgtca gtcgcagcgc
      781 cggatgccag cgctggatgc gctcgagcgc gcggcgccag aacggcgggc cggcgtgcag
      841 ggcgccgtgc ggcgccggcg tggagggtgg ctcgcggtcg ggcgcggggg tcacgccgcg
      901 actcgcgatg gcgggaagat cagttctgga cgaactgcat ctgcgtgccg gccaggtcga
      961 tgatgtcgcc gtgcttcagc gagatcgcct cttcgccgac aggctgccca ttgacgttgg
     1021 ggcggcgcga gccctcgaca tgcgacagca cgtagccgcc cgggcgcttg gtgaccgagg
     1081 ccacctgcac gcccggcttg ccgaccgtgg tgacgacctt ggtcagcagc acttcgcggc
     1141 cggcggcgcc gccgttgagc accttgatgg acgccgggcc ggcggccgcc ggcgtggccg
     1201 ccgcagcgga gccgggcgcg cccggcggga cggcgcccgg cgcgcggccg aacgcgcccg
     1261 ggcgcaggat catcgtcttt tcatagtcct cggcctcctc ggccaggaac ttgatgcggt
     1321 acttgccgac ctcgaccacg tcgttgttgg acagcagctg cttcttgacg gccttgccgt
     1381 tgatgtaggt gccgttggtg ctgttcaggt cttcgatgta gacatccgac tgcgccgccg
     1441 tcaggaccgc gtgctcgccg ctgacggcga ggttgtcgat cacgatgtcg ttgtacggcc
     1501 ggcggccgag cgttgtgcgc tccttcgtga tctggaattc cttgatcacg acgttatcga
     1561 gcgagacgac gagtttgccc attgaaaacc tcagtttctc tcgcggccta ttttgtccgc
     1621 ttgtcggaaa cgccgcagcg ctctttttaa ttcatcgtcc ggtatgtgaa gaccggtctg
     1681 tcaacgccag aaggaccacc agctcttgcc gaaaccctgg gtgcccgcct tggcccgagc
     1741 cagtatcacc gcgatattat cgcgccctcc agcggcgttg gcggcctgaa ccagcatcgc
     1801 acccgccgcc gggaggctgt cgttggtgcg caacagctgt tcgatctgct cgtcggagag
     1861 catgtcggac aggccgtcgg agcacatcaa cagcagatcg cccggctcga ccggataatc
     1921 gtgtatttcg aggtcgacga gttcgtccac gcccaccgca cgtgtaacga gattcttgtg
     1981 catggagtag gccgcctgtt ccgccgtgat caggccggcg tcgatctgct cctgcagcag
     2041 cgagtggtcg cgggtcagct gctcgaggtg gccgttgcgg aatcggtagc cgcgcgagtc
     2101 gccgatgtgg cccagccaga ggcgctcgcc ccggtagacg gccaccacca gcgtggtgcc
     2161 catgcccgcg tattcggggt gggacatcgc ggcgtcgtag acctcgcggt tggccgaatc
     2221 gacggcgtcg agcagcgccg acttcatctc cgagtcggtg ggcgtggcgc ccaggccctt
     2281 cagccgctgt cccagctcgc ccttgacgcg ctcggaggcc atgccgctgg cgacctcgcc
     2341 cgcgttgtag ccgcccatgc cgtcggccag cacggcaatc cgccccgcct cgtcgaccgc
     2401 cacggagtct tcgttgttgc ggcgcgcacg gccggtgtcg gtaacgctga agaactcaag
     2461 aatcatgagg acggcagggt gcggcaaggc gctgaaagac gccaatcagc tggcgattgt
     2521 gccttggaat gaggaagatg ccgtcaactc atgcagggga tcgtcgccgg acacggtatc
     2581 gggccaatcg gcggccgcgc tgcccggctc ggcggcggcg cactgctggc gggccaggcg
     2641 cagttcgagc acgacctggc gggcgctttc ctggcgcagg cccgggctct tggcgagcag
     2701 gcgggcgacc acgtcgtcca gcagcggcgg cagctccggg cgcagctcgc gcacgcgggg
     2761 cgcgtcctgg tgcgcgatgc gcgcgagcag gtcgcccatc gagtcggact cgaacggccg
     2821 gcggccgatc aacagctcga acagcaggac gcccagcgcg tagaggtcgc attgggccgt
     2881 ggcgtcggcc ccggccagtt gctcgggcgc catgtaggcc ggggtgccca gcatgacgcc
     2941 actgcgggtg gcggccatgt cggccacgcg ggcggagccg aaatcggtca ccttgacgac
     3001 gccggtggtc gggtcgaaca ggacgttgga cggcttgatg tcgcggtgga cgatgcccga
     3061 gcggtgcgcc aggtcgaggg cgagcgccac gcgctggcag atgcccacca ccacgccttc
     3121 gggcagcagc cggttggcgc tggcgtagcg cgacatgtcc gtgccgtgca cccactccat
     3181 ggccagccag ccggtcgtgg cgtcgccctc gccctgcacg ccgccggcat gcaccgtgac
     3241 gatgtagtcg tgcttgagcc gcgcggcggc ctcggcctcg cgcaggaagc gctcgcgcca
     3301 gaggccgcgg tcggtgccgc tggcgctgcc gatgcgcaca gtcttgagcg cgatcagctg
     3361 gccgctggcg cggtcggtgg cctggtagat ggtggcgcgg tcgctgcggg cgatgacgtg
     3421 ctgcagcgcg tagtcggtca ccttgggcat gtcggtcgcc gggccgaact gcggcacctg
     3481 caggtgctgc atcgcggggg cggccgcggc gccgggtgcg agcccgaaca tgcgcttcaa
     3541 gcgggaccac gccggcggcg ccgcctgggg cgacggtgcg ggcttgggga gcgaggaacg
     3601 cggcatcctg caagattgtc ggcagtcggc gcgcccgtca acccgccgat atcgatagcc
     3661 gcgctcaatg cggctttcgt cacgcgctgc tgagcaggcg tccggtcagg cgcgcgcctt
     3721 cgcccgcctt tgcagcagct tgcccgcgat cacgacgagg atcgcgccga cgatgccggc
     3781 gatgcgcacg gcgttggcgg atggctcgcc gaggcgcggc gggatggccg gatcggtgag
     3841 catcatctcg cccgccagcc agcccagcag tgcggcgccg gcgacgatga tggccgggaa
     3901 gcgctccatc accttgagga tgatcgtgct gccgaagacg atcagcggga tgctgaccgc
     3961 caggccgatc accagcagcg gcgtgttgcc ctgggccgcg gccgccacgg ccagcacgtt
     4021 gtccaggctc atcacgagat cggccacgag gatggtgcgc acggccgcgc cgatgccgat
     4081 cggccgggcc tcctggtcgt gctgctcctc ctcttcggac atcaggttcg cgccgatcca
     4141 cagcagcgcg atgccgccga cgagcttgag ccacggcagg cccagcatct tcacggcgat
     4201 cagcgtcagc acgatccgca tgacgatggc cgccccgctg ccgaacacga tggccttctt
     4261 ctgctggcgg gcgggcagcc cgcgcgcggc catcgcgatg accacggcgt tgtcccccga
     4321 cagcaggacg ttggccagga tgatggagcc gagggcggcc cagaaggcag gggtcagcag
     4381 gaggtccaag atcgcgcggg cggcaggagg tgagtgggtc gggcaaaaga aaaggagccc
     4441 gtgggctcct cgtcgtgccg cttcgcagtc tacccgcgga cggcaagctt gcgttcagct
     4501 ccgcgcgagg gtcgcgcgaa accgccggtt gcaacttcgt cagccgatca cagcagggcc
     4561 ttgagcagct tgcccatctc ggacgggttg cgcgtgacct tgaagccgca ctcttccatg
     4621 atggcgagct tggcgtcggc cgtgtcggcg ccgcccgaga tcagcgcgcc ggcgtggccc
     4681 atgcgcttgc cgggaggggc ggtgacgccc gcgatgaaac cgacgatcgg cttcttcatg
     4741 ttgcccttgc accagcgcgc cgcgtcggcc tcgtccgggc caccgatctc gccgatcatg
     4801 atgacggcgt cggtctcggg atcgtcgttg aacgccttca tcacgtcgat gtgcttcagg
     4861 ccgttgatcg ggtcgccgcc gatgcccacc gccgacgact ggcccaggcc gatctcggtc
     4921 agctgcgcca cggcttcgta cgtcagcgtg cccgagcgcg agacgacgcc gatgcggccc
     4981 ttgcgatgga tgtggccggg catgatgccg atcttgatct cgtcgggcgt gatcaggccg
     5041 gggcagttcg ggcccagcag cagcgtcttc ttgccgccgg cggcttcctt ggccttcatc
     5101 ttgttgcgca ccatcagcat gtcgcgcacg gggatgcctt cggtgatgca gaccaccagg
     5161 tccaggtcgg cctcgacggc ttcccagatc gcggccgcgg cgcccgcggg cggcacgtag
     5221 atgaccgaca cgtcggcgcc ggtctgctgc ttggcttcgg cgacggtggc gaagatgggg
     5281 atgtccgaga acttctcgcc cgccttcttc gggttcacgc cggcgacgaa cgcgttcttg
     5341 ccgttcgcgt aggcctggca gccgagggtg tggaactgcc cggtcttgcc cgtgatgccc
     5401 tgggtgatga ccttcgtgtc cttgttgatg tagatcgaca tttcgcttga ttccttggtc
     5461 tgggctgggc ttacttgacg gcggcaacga tcttcgttgc ggcttcggcc atcgtgtcgg
     5521 cggcgatgat gggcaggccc gattcggcca gcatcttctt gcccagttcc tcgttcgtgc
     5581 ccttcatgcg cacgaccagc ggcacctgca ggttcacggc cttgcaggcg gtgatgacgc
     5641 cttcggcgat ggtgtcgcac ttcatgatgc cgccgaagat gttgaccagg atgcccttga
     5701 cgtgcgggtt cttgagcatg atcttgaagg cttcggtcac cttctcggcc gtggcgccgc
     5761 cgccgacgtc caggaagttg gccggctcgc cgccgaacag cttgatggta tccatcgtgg
     5821 ccatggccag tccggcgccg ttgaccaggc agccgatgtt gccgtccagg ctgatgtagg
     5881 cgaggtcgaa cttcgaggct tcgatctccg ccgggtcttc ttcgtcgagg tcgcggtacg
     5941 cgacgatctc ggggtggcgg aacagcgcgt tggcgtcgaa gttgaacttg gcgtccagcg
     6001 cgatcaggtt gcccttgctg tcgcagttga gcgggttgat ctcgaccagc gaggcgtcgg
     6061 tgtccatgta gcactggtag agcttctgga acaggtcgac ggcctgggcc tgcgagcctt
     6121 cgggcaggcc gatggcggcg gcgatcttct ttgcctggtc ggccttcagg ccggtcagcg
     6181 gatcgatgac ctcggtgatg atcttctcgg gcgtggagtg ggccacttcc tcgatgtcca
     6241 tgccgccttc gctggaggcg atgaaggcga ccttctgcgt cgcgcggtcg gtgaccagcg
     6301 agacgtagta ttccttcttg atgtccgcgc cttcctcgat gtacaggcga cggaccttct
     6361 ggccttcggg gccggtctgg tgcgtcttga gctgcatgcc caggatctcg ccggacagct
     6421 tcttgaggtc gtccatcgaa cgggcgagct tgacgccacc gcccttgccg cggccgccgg
     6481 cgtggatctg ggccttgacg acccagacgc cgccgccgag tttctgagcg gcttccaccg
     6541 cctcctgcac cgtgaaggcg ggatagccgc gcggcacggg cacgccgaac tggcgcagga
     6601 gttccttgcc ttggtattcg tggatcttca tggaagcgtc ttctcgaagt gatcgactaa
     6661 agggagtgtc cggggagctg cgcgtcgcgt cgggcacgcg cgaagcgcgg gcgaggcgag
     6721 ggagaggcag tggacgggat taacgcaagc gggagatttt gcgatggatt gccacatccg
     6781 ggcgagcctc cgcagggcgc ggatgccagt aaacccgatg taaccacgaa ctttatcatg
     6841 gtgcaccgca ccaagtccga cccgtaggtg gtcacccgta tggcggcggg gtgtcgcggc
     6901 gggcgctggc gagccgggtg gaactcgccg tcaatcctcg tcgaggccgc cgacgacgcg
     6961 gcgcaccacg tcggccgcaa agccacgcgc ggccaggaag cgcgcctggc gggcacgctg
     7021 cctcggatcc gccgccacct cgccgaactt gcgctgccac agctcgcggg cgcgggcgaa
     7081 ctccgtctcg cgcagggccg tggcctgttc gggctcgagc gcgagaccgt ggcgcgacag
     7141 ctccagcttg atgcgcagcg tgccctgctt gcgcgagcgg gcgttgactc ggctctcgac
     7201 gaagcgggcg tcgctgaggt acttgttgac gacgagccag tccagcaggc ggtcgacggc
     7261 ggtctcgggg tcggtgcgtt cgaggtcggc atcgtccggg cagtcgcggg gaacgacggg
     7321 ctcactggac gcgtggtcgg accggccggc gtcctcgaga acatcaccgg acggattggc
     7381 gggaaccgcg cggccgggcc gcctctgcga agcagcagga tcggccgacg agccggcgcc
     7441 ggcttccagg cacgcacgaa ggtgggcggg cagtgcggcg ccggtcgcga acgtcagcgt
     7501 gtcgagcgct tgcgactgcg ggcgagcgcc cgggcgcgac gcctgcgtcc gcgcgggcgt
     7561 tggcgacggc tcggctgcgc gcggcgcgaa cgcgtcgtcg atgccgttgg acggctgcgc
     7621 cagtgcggcc tggcgcacct cctccgccgc cgcttccagc gccgcgtcct cgcgggcgcg
     7681 cttgcgcagc gattccagca gcttcgcgcg caactcctgg cggctgtgct cgcgccgcga
     7741 caggaaaccg atggcctgca tcttgaggct ctggcgcgga cgcgcgcgcg tccggcgcgg
     7801 cgcggaaggt tcccgtgggt tgtcgggaag cgacatggca ggggtggatc ctgcgactac
     7861 gcgcaggatg acaatgtcgc catcccgcgc acgtcagcgc acgccgcgct cactcggccg
     7921 cggcggcctt gcccttcttg gcggcgggcg cctcgacgac cggcaccagc ggcacgccca
     7981 gcgattcgcg caccttgttc tcgatctcga tggccagcgc cgggttctcc ttcaggaact
     8041 cgcggcagtt gtccttgccc tggccgatct tctcgccgtt gtaggcatac caggcgccgg
     8101 acttctcgat gaccttggcg ttgacgccca ggtcgatgat ctcgccttcg cggctgatgc
     8161 cttcgccgta caggatgtcg aactcggctt ccttgaacgg gggcgagacc ttgttcttga
     8221 cgaccttgac cttggtctcg ttgccga
//
LOCUS       DATCRO010000433         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_961247, whole genome shotgun sequence.
ACCESSION   DATCRO010000433 DATCRO010000000
VERSION     DATCRO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961247"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VIN75_11720"
     CDS             <1..1698
                     /locus_tag="VIN75_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY8974880.1"
                     /translation="LYKQTQLQDSFGMNMVALVDGQPKVCNLKLLISIFLEHRREVVT
                     RRTVYELRKARDRGHVLEGLAVALANIDDFITLIKESPTPPVAKTALMSRGWDSSLVR
                     EMLERAGADSPAGAGAFRPDGLPDHYGLQGDGMYRLSETQAQEILQMRLQRLTGLEQD
                     KIVGEYRDVMKEIADLLDILARKERVTTIIGDELLALKAEFGTGKLAVRRSQIEHNTQ
                     ELGTEDLITPTDMVVTLSHTGYFKSQPLAEYRAQRRGGRGKQATQTKDDDWVDQLFIA
                     NTHDWILCFSDAGRVYWLKVWEVPQGGRNSRGKPIVNMFPLQKDEKITVVLPLTGEFR
                     SFPSDHFIFMATSQGTVKKTPLDDFSNPRKAGIIAVTLDEGDHLIGAALTDGQHDVML
                     FSDGGKAVRFDEADVTPHGRTSRGVKGMTLEAGQSVIAMLVAEDETQSVLTATENGFG
                     KRTSIVEYTRHGRGTKGMIAIQQSERNGKVVAATLVRPNDEIMLITDKGVLVRTRVSE
                     VRELGRATQGVTLISLDDGAKLSGLQRIVENDANVENGDNGDNGADGEAPSGDEPAA"
     gene            1703..2803
                     /gene="serC"
                     /locus_tag="VIN75_11725"
     CDS             1703..2803
                     /gene="serC"
                     /locus_tag="VIN75_11725"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY8974881.1"
                     /translation="MADHTRPYNFAPGPAAIPEPVLREAAADMLDWHGCGVGVMEMSH
                     RGKEFMQIAAEAQADLRELLAVPSNYRILFMQGGGLGENAIVPMNLSRGGQVDFVQTG
                     GWSRKSIAEARRYADVAIAASNAGTADSAIPDPSTWTLRPGASYVHICSNETVDGIEF
                     QSLPDLKALGSDAPLVIDCSSHILSRPIDWSRVGAAFAGAQKNVGPAGLTMVFVREDL
                     IGHALPITPSVFDYRVVADNDSMFNTPPTWAIYIAGLTFKWIKAQGGVAAMEARAIEK
                     ATMLYAALDASDVYVNRVARNSRSRMNVPFWLRDESLDDTFLAGARERGLLALKGHKS
                     VGGMRASIYNAMPVDGVKALVEYLNEFAARHG"
     gene            2796..3899
                     /gene="pheA"
                     /locus_tag="VIN75_11730"
     CDS             2796..3899
                     /gene="pheA"
                     /locus_tag="VIN75_11730"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY8974882.1"
                     /translation="MAENDAPNPELLALRVQIDALDRELLEALNRRARLAQTVGEVKK
                     REGSVAFRPEREAQVIDGLKAVNPGPLKTESVAPIWREIMSACRALETPTRVAYLGPA
                     GTFSEEAALGYFGSSIVRVPCASVDEVFRVTTAGAADFGVVPVENSTEGMVARSLDQF
                     LVTPLFIIGETSLFVRHNLLRKTNSLEGISAVCAHPQALAQCHMWLNDHLPHAERRPV
                     ASNAEGARLASLDPTLAAIASTRAGSEYGLHVVSPAIQDDANNRTRFAVITHTERHPA
                     PKASGHDCTSLVVTVSNRPGALHDMLVPLKANGVSMTKFESRPARSGQWEYYFYVDIA
                     GHPDQDHVRQALEDLRKVCGFFKILGTYPVDVH"
BASE COUNT          688 a         1449 c         1364 g          545 t
ORIGIN      
        1 ctgtacaagc agacgcagct gcaggactcg ttcggcatga acatggtggc gctggtcgac
       61 ggccagccca aggtgtgcaa cctcaagctg ctgatctcga tcttcctgga gcaccgccgc
      121 gaagtggtga cgcgccgcac ggtgtacgag ctgcgcaagg cgcgcgaccg cggccacgtg
      181 ctggaagggc tggcggtggc gctggccaac atcgacgact tcatcacgct gatcaaggag
      241 tcgccgacgc cgccggtggc caagacggcg ctgatgtcgc gcggctggga ttcctcgctg
      301 gtgcgcgaga tgctcgagcg cgcgggcgcc gactcccccg ccggcgccgg cgcgttccgc
      361 cccgacggcc tgcccgacca ctacggcctg cagggcgacg gcatgtaccg cctgtccgag
      421 acgcaggccc aggaaatcct gcagatgcgc ctgcagcgcc tcacgggcct ggagcaggac
      481 aagatcgtcg gcgagtaccg cgacgtcatg aaggagatcg ccgacctgct ggacatcctg
      541 gccaggaagg aacgcgtcac caccatcatc ggcgacgagc tgctcgcgct gaaggcggag
      601 ttcggcaccg gcaagctcgc ggtgcgccgc agccagatcg agcacaacac gcaggaactg
      661 ggcaccgagg acctgatcac gcccaccgac atggtggtca cgctgagcca caccggctac
      721 ttcaagagcc agccgctggc cgaataccgc gcgcagcgcc gcggcggccg cggcaagcag
      781 gccacgcaga ccaaggacga cgactgggtc gaccagctgt tcatcgcgaa cacgcacgac
      841 tggatcctgt gcttctccga cgccggccgc gtttactggc tcaaggtgtg ggaggtgccg
      901 cagggcggcc gcaactcgcg cggcaagccc atcgtcaaca tgttcccgct gcagaaggac
      961 gagaagatca ccgtggtgct gccgctcacg ggcgagttcc gcagcttccc gtcggatcac
     1021 ttcatcttca tggccacctc gcagggcacg gtcaagaaga cgccgctgga cgacttcagc
     1081 aacccgcgca aggccggcat catcgccgtc accctcgacg agggcgacca cctcatcggc
     1141 gccgcgctga ccgacggcca gcacgacgtg atgctgttct ccgacggcgg caaggccgtg
     1201 cgcttcgacg aggccgacgt gacgccgcac gggcgcacgt cgcgcggcgt caagggcatg
     1261 acgctcgagg ccggccagtc ggtcatcgcg atgctggtgg ccgaggacga gacccagagc
     1321 gtgctcaccg ccaccgagaa cggcttcggc aagcgcacgt ccatcgtcga atacacgcgc
     1381 catggccgcg gcaccaaggg catgatcgcg atccagcaga gcgagcgcaa cggcaaggtc
     1441 gtcgcggcca cgctggtgcg cccgaacgac gagatcatgc tcatcaccga caagggcgtg
     1501 ctcgtgcgca cgcgcgtgag cgaggtgcgc gagctcgggc gcgcgacgca gggcgtcacg
     1561 ctgatctcgc tggacgacgg cgccaagctg tcgggcctgc agcgcatcgt cgagaacgac
     1621 gcgaacgtcg agaacggcga caacggcgac aacggcgccg acggcgaagc cccgagcggc
     1681 gacgaaccgg cggcctgacg cgatggccga ccacacgcgc ccctacaact tcgcgcccgg
     1741 ccccgccgcg atccccgagc cggtgctgcg cgaagcggcc gccgacatgc tcgactggca
     1801 tggctgcggc gtcggcgtga tggagatgag ccatcgcggc aaggagttca tgcagatcgc
     1861 ggccgaggcc caggccgacc tgcgcgagct gctggccgtg ccctcgaact accgcatcct
     1921 gttcatgcag ggcggcgggc tcggcgagaa cgccatcgtg ccgatgaacc tgtcgcgcgg
     1981 cggccaggtc gacttcgtgc agaccggcgg ctggtcgagg aagtccatcg ccgaggcgcg
     2041 ccgctacgcg gacgtcgcca tcgccgcgag caacgccggc accgccgaca gcgcgatccc
     2101 cgatccgtcc acctggacgc tgcgcccggg cgcctcgtac gtgcacatct gcagcaacga
     2161 gacggtcgac ggcatcgagt tccagtccct gcccgacctg aaggcgctgg gcagcgacgc
     2221 gccgctggtg atcgactgct cgtcgcacat cctgtcgcgg cccatcgact ggtcgcgcgt
     2281 gggcgcggcc ttcgccggcg cgcagaagaa cgtcggcccc gcggggctga cgatggtctt
     2341 cgtgcgcgag gacctgatcg gccatgcgct gccgatcacg ccgtcggtat tcgactaccg
     2401 ggtcgtcgcg gacaacgact ccatgttcaa cacgccgccg acgtgggcga tctacatcgc
     2461 gggcctgacc ttcaagtgga tcaaggccca gggcggcgtc gcggcgatgg aagcgcgcgc
     2521 gatcgagaag gccacgatgc tgtatgcggc gctcgacgcg agcgacgtct acgtcaaccg
     2581 cgtcgccagg aactcgcgct cgcgcatgaa cgtgccgttc tggctgcgcg acgagtcgct
     2641 cgacgacacg ttcctggccg gcgccaggga gcgcggcctg ctcgcgctca agggccacaa
     2701 gagcgtgggc ggcatgcgcg cctccatcta caacgcgatg cccgtcgacg gcgtgaaggc
     2761 gctggtcgag tacctcaacg aattcgccgc gcgccatggc tgagaacgat gctcccaacc
     2821 cggaactgct cgcgctgcgc gtgcagatcg acgccctcga ccgcgagctg ctcgaagcgc
     2881 tgaaccgccg cgccaggctc gcgcagacgg tgggcgaggt caagaagcgc gagggctcgg
     2941 tggccttccg ccccgagcgc gaggcgcagg tgatcgacgg cctcaaggcg gtcaaccccg
     3001 gcccgctcaa gaccgagagc gtggcgccga tctggcgcga gatcatgtcg gcctgccgcg
     3061 cgctggagac gcccacgcgc gtggcctacc tcggccccgc gggcacgttc agcgaggagg
     3121 cggcgctcgg ctacttcggc tcgtccatcg tgcgcgtgcc gtgcgccagc gtcgacgagg
     3181 tgttccgcgt gaccaccgcc ggcgccgccg acttcggcgt cgtgccggtc gagaactcca
     3241 ccgagggcat ggtcgcgcgc tcgctcgacc agttcctcgt cacgccgctg ttcatcatcg
     3301 gcgagaccag cctgttcgtg cgccacaacc tgctgcgcaa gacgaactcg ctcgaaggca
     3361 tctccgccgt ctgcgcgcac ccgcaggcgc tggcgcaatg ccacatgtgg ctcaacgacc
     3421 acctgccgca cgccgagcgc cgcccggtcg cgagcaacgc cgaaggggca cgcctggcct
     3481 cgctcgaccc gacgctggcc gccatcgcga gcacgcgcgc cggcagcgag tacggcctgc
     3541 acgtggtgtc gcccgcgatc caggacgacg ccaacaaccg cacgcgcttc gccgtcatca
     3601 cgcacaccga gcgccatccc gcgcccaagg cgtccggcca cgactgcacc agcctcgtcg
     3661 tgacggtgtc gaaccgcccc ggcgcgctgc acgacatgct cgtgcccctg aaggccaacg
     3721 gcgtgtcgat gaccaagttc gagtcgcgcc cggcgcgctc tggccagtgg gagtactact
     3781 tctacgtcga catcgcgggc caccccgacc aggaccacgt gcgccaggcg ctcgaggacc
     3841 tgcgcaaggt gtgcgggttc ttcaagatcc tggggacgta tccggtcgac gtgcactgac
     3901 gggcgatgtc cggcctttcc tggaacatcg gcattccctg cgggacggcg ttcggccgcc
     3961 gctagctcgc gaccctgcag ccgggcgtcc ctcccggcct cgaaggcttc tgagacgagc
     4021 gcagaagccg cgacgatcgc ctacgc
//
LOCUS       DATCRO010000434         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_826131, whole genome shotgun sequence.
ACCESSION   DATCRO010000434 DATCRO010000000
VERSION     DATCRO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826131"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VIN75_11735"
     CDS             <1..1312
                     /locus_tag="VIN75_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974883.1"
                     /translation="ESSDLLHSPTEAMYTRSFTAPRWGVRSTWRSASLAGTAFVIDDK
                     GGGETLLPHGYGTDTALQPGSRSLSARVHADLDDAARFQLGGILAARRYEGDRGDNSV
                     AGPDFNWQLDDAWRVKGQWLRSQTTAQPFTDGATGDVTLAKGATTTGDSVFLKLMRYT
                     DRTQLEISDLDISPQFRHDTGFVNQVGIRDIEVHPSMVFRDLAPINEMWLNLRGSLVR
                     DRINGDVIQESLAPDVWVSLPRNTQAWLSWHGLSKVRATSGGRLLAEHYWKGEYTSTL
                     AQSIPLFDATVSLGRMADVTAGVVRPGGQVNLMLSTRPFAAIEIEPSIWAAWLKNGSL
                     LAYRETASQLLGVWHIDARQNLRLIVQRSTVDRKAEAGVAADRESGLTTSLTYALRKS
                     MGTVVYVGATRSHSGVGAQNIARGTEAFVKLQVDVDEVRGMFGH"
     gene            complement(1367..1867)
                     /locus_tag="VIN75_11740"
     CDS             complement(1367..1867)
                     /locus_tag="VIN75_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8974884.1"
                     /translation="MKAKTSPTAAELAQLDTYHAQVRGHLDRLAALASRIENGQLDAD
                     TGAEACAVEAFFSGSMQEHHRVEEAGVFPFLLASDDKELVDVVRTLQQDHGWMEQNWI
                     ELAPQLRAIAQGNSWIDPAEFMHGAEVFLELSYGHSALEEAVVYPRAQAARDAQGARQ
                     VAGTPG"
BASE COUNT          313 a          745 c          761 g          319 t
ORIGIN      
        1 cgagtccagc gacctgctgc actcgcccac cgaggcgatg tacacgcgca gcttcacggc
       61 gccgcgctgg ggcgtgcgct cgacgtggcg cagcgcgtcg ctggccggca cggcgttcgt
      121 catcgacgac aagggcggcg gcgagacgct gctgccccac ggctacggca ccgacaccgc
      181 gcttcagccg ggctcgcgct cgctgtccgc gcgcgtgcac gccgacctcg acgacgcggc
      241 gaggttccag ctgggcggca tcctggccgc gcgccgctac gagggcgatc gcggcgacaa
      301 cagcgtcgcc ggacccgact tcaactggca gctcgacgac gcctggcgcg tgaagggcca
      361 gtggctgcgc tcgcagacga cggcgcagcc gttcacggac ggcgccaccg gcgacgtcac
      421 cctcgccaag ggggcgacga ccaccggcga ctcggtgttc ctcaagctta tgcgctacac
      481 cgaccgcacg cagctggaga tctccgacct cgacatcagc ccgcagttcc gccacgacac
      541 gggcttcgtc aaccaggtgg gcatccgcga catcgaggtc cacccgtcga tggtgttccg
      601 cgacctcgcg cccatcaacg agatgtggct gaacctgcgc ggctcgctgg tgcgcgaccg
      661 catcaacggc gacgtgatcc aggaatcgct ggcgccggac gtgtgggtga gcctgccgcg
      721 caacacgcag gcctggctct cgtggcacgg gctgtccaag gtgcgcgcga cctccggtgg
      781 gcgcctgctg gccgagcact actggaaggg cgagtacacg tcgacgctgg cgcagtccat
      841 tccgctgttc gacgccaccg tctcgctcgg caggatggcc gacgtcacgg ccggcgtcgt
      901 gcgtccgggc gggcaggtca acctgatgct gtccacccgc ccgttcgcgg cgatcgagat
      961 cgagccgagc atctgggcgg cgtggctgaa gaacggctcg ctgctggcct atcgcgagac
     1021 ggcgtcgcag ctgctgggcg tgtggcacat cgacgcgcgg cagaacctgc gcctgatcgt
     1081 gcaacgctcc accgtcgacc gcaaggccga ggcgggcgtc gcggccgacc gcgagtcggg
     1141 cctgacgacg tcgctgacct acgcgttgcg caagtcgatg ggcacggtgg tgtacgtcgg
     1201 cgcgacgcgc tcgcacagcg gcgtcggtgc gcagaacatc gcccgcggca ccgaggcgtt
     1261 cgtcaagctg caggtggacg tcgacgaggt gcggggcatg ttcgggcact gacggcgcac
     1321 cgggcggctc ccgaagggtc cggcatcgtc gcgccaccgc gggaattcag cccggcgtgc
     1381 cggccacctg gcgggcgccc tgggcatccc gcgccgcctg ggcccgcggg tagacgacgg
     1441 cctcctcgag cgccgagtgc ccgtagctca gctcgaggaa cacctcggcg ccgtgcatga
     1501 actcggccgg gtcgatccag ctgttgccct gggcgatggc gcgcagctgc ggcgcgagct
     1561 cgatccagtt ctgctccatc cagccgtggt cctgctgcag ggtgcgcacg acatcgacca
     1621 gttccttgtc gtcgctggcc agcaggaacg ggaacacgcc ggcctcctcg acgcggtggt
     1681 gctcctgcat cgagccggag aagaacgcct cgacggcgca ggcttcggcg ccggtgtccg
     1741 cgtcgagctg gccgttctcg atccgggagg cgagggcggc caggcgatcg aggtggccgc
     1801 gtacctgggc gtggtaggtg tccagttggg cgagttcggc ggccgtgggg ctggtcttgg
     1861 ctttcatcgc gggctcctgg acgaaggatg cttcctttat acgccgccgc gtggctgccg
     1921 gggggctttc gcggcaacgt ttgacctgag cccagggcaa atccgatcgg cctagacttg
     1981 ggacgccccg ttcggcgcac acgcgcgccg ggcccgcgcg aaggagactg caacgtgcaa
     2041 atcgccaaca gtgcctcgcc cgactatttc cacaaggtgg tcgactgcca gtgggcgtgt
     2101 cccgcgcaca cgccggtgcc ggaatacatc cggctgat
//
LOCUS       DATCRO010000435         9137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_297152, whole genome shotgun sequence.
ACCESSION   DATCRO010000435 DATCRO010000000
VERSION     DATCRO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297152"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIN75_11745"
     CDS             complement(<1..468)
                     /locus_tag="VIN75_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8974885.1"
                     /translation="MDQTVINILNGLSSGLLLFMLSSGLTLIFSMMGVLNFAHAGFYM
                     LGAYVGYASSRIVGFWPALAVAPVVVGVLGAAFERLCLRRVHRHGHVPELLVTFGLSY
                     LILELVQVVWGRAPVAFDPPAALSRPAFTLVQSAATGLSVALGQADAATCAHAA"
     gene            complement(824..2074)
                     /locus_tag="VIN75_11750"
     CDS             complement(824..2074)
                     /locus_tag="VIN75_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8974886.1"
                     /translation="MTFLSKPLAFASTLGLACLALHPGAAFAQKGETVKIGWIDPLSG
                     LMAPIGQNELKTFQYLAELFNKNNPAGVKFEIVPFDNKVSPAESLTALKSAIDQDIRY
                     ITQGDGSGAAGALVDAVNKHNERNPGHEVLYMNYAAVDPDLTNGKCSYWHFRLDADTS
                     MKMEALTTFIKDQKDIRKVYILGQNYAHGLQVAKYAKADLAHKRPDIEIVGEDLHPLA
                     KVTDFAPYVAKIKASGADTVITGNWGTDLSLLVKAANEAGLNVKFFTYYANNSGTPTA
                     IGAAGAGRVYFVGYGHPNMSGPIRKLQDDFKARFNDDLYVADLYTELQLLSEAMAKAR
                     STDPVKVAAALEGMKLKSYNGDIEMRRTDHQLQQDLWISVWQKTDAKNPYSVENTGYT
                     FAPVAHYDAYVASTPTSCQMKRPS"
     gene            complement(2201..3853)
                     /locus_tag="VIN75_11755"
     CDS             complement(2201..3853)
                     /locus_tag="VIN75_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEY8974887.1"
                     /translation="MTVLMGQMMNMPLTVSSMIRHAARHAGEVEIVSKRCEGDIHRSN
                     WAQVEKRARQLAQALRALAVAPGERVATLAWNGHRHVEIYYGVSGSGRVCHTINPRLF
                     PEQIEWIANDAEDAVLCFDSGFLPLVEKLAPKLKTVRHFILMGHRGQMPASTSLPNLL
                     CFDDLLAGHAGDYDWPELEEQTAATLCYTSGTTGNPKGALYSHRSIVLHSYAAALPDA
                     MNCSTRDVILPIVPMFHVNAWGIPYTAAMVGAKLVMPGPFLDGASLYDLFESEKVTFS
                     AGVPTVWLGLLNYMKANDLKFSTFKRTVIGGSACPPAMMKSLQQDYGVEVIHAWGMTE
                     LSPLGTLSKLQSKHEALPAEEKQRLLQSQGRVIFGIDMAIIDDEGRSLPWDGKAAGNL
                     VVRGPWVISSYFKSKDSPLVSVDGEPGWFPTGDVAAIDADGFMFITDRSKDVIKSGGE
                     WISSIELENIAIAHPSVHEAAVIACKHPKWDERPLLVVVPKPGATVTREELLRFFDGK
                     IAKWQIPDDVAVVAELPHTATGKLQKLRLREQFRDHVLPTAG"
     gene            3986..5563
                     /locus_tag="VIN75_11760"
     CDS             3986..5563
                     /locus_tag="VIN75_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY8974888.1"
                     /translation="MSDIDSAQAPTGPDAARWINRFARFAHDVPDVRRDAESIPAETR
                     ARHPFGTDLPAQPFPEPHWVATSDDCAALLGWPSDWATRADWRALDVLTGRATWPGLH
                     TMATVYSGHQFGVWAGQLGDGRALLLGEWQPEAGGPSFEIQLKGAGRTPYSRMGDGRA
                     VLRSSIREFLCSEAMHALGVPTTRALCVAGSALPVMRETPETGAVVTRVAPSFLRFGH
                     YEHFAHSGQHESLRRLVDFTIAAYFPDLRDGADGLDPLHAWLAEVVRRTAATVAHWQA
                     VGFAHGVMNTDNMSILGLTIDYGPFGFLDGFDPGHICNHSDTGGRYAFARQPNIAWWN
                     LHALAHALRPLCADPDTTLPPVLDAFSDRFIDAYSDHMRAKLGLVLAQDGDKELLDDL
                     LQLMAGERVDMTIAFRALARFDRGAGATPAAFRDHFIDRAAADAWGERYLRRLEAEAR
                     PDAERHAAMDRANPKFVLRNHLAQEAIAKAEAGDFGELRQLHDLLRRPYDEQPGRERY
                     AALPPDWARHIEVSCSS"
     gene            5700..6338
                     /locus_tag="VIN75_11765"
     CDS             5700..6338
                     /locus_tag="VIN75_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY8974889.1"
                     /translation="MKLLFDILPLIFFFATYKIAGRMPDQGAAFATHWLGAVVQGGVV
                     GSTEAPALLATAVVIAATLVQVSWYKIAGRKIDLMLWVSTAMIIVFGGLAVWFHNQMF
                     IMWKPSIYFWVTGLIFWGSQTFFHKNLWRATLGNDMVVPDTVWQRFNFAWVAFFALMG
                     LVNLLVVYFFRDYWVGFHTFGTTALMAVFLLVAFYYLGKHVEPELTADKPAP"
     gene            6368..6613
                     /locus_tag="VIN75_11770"
     CDS             6368..6613
                     /locus_tag="VIN75_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY8974890.1"
                     /translation="MTQALTERLEPLVLELVDDSAAHAGHAGAREGAHFNLRIVSAKF
                     AGLPRISRHRLVYDALRPWMAEGVHALAIDARTPEEA"
     gene            6800..7666
                     /locus_tag="VIN75_11775"
     CDS             6800..7666
                     /locus_tag="VIN75_11775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8974891.1"
                     /translation="MNFKSSTALIAALVLPLSVMAQNVATVNGKPVPMSRVDALIKTA
                     THGQQQDVPPEVKAQAKDQVVLREIFAQEAEKQGIPQSADYKAQLELVRQTVLINTLF
                     QNFIKTHPVSDAEAKAEYDQIKAEQSALEYHARHILVDSEDEAKKLIAQIKAGSKFED
                     VAKKSSKDTESAEKGGDLDWAKPSNYVPEFATAMQGLKKGQMTDTPVKSQFGYHIIRL
                     EDTRAAQFPSFDEVKDKIKQQKEQVKLQEYQEKLRKSAKTDYKFAAEQQQQQQQQAPA
                     APAAAPASAAKK"
     gene            7818..8846
                     /locus_tag="VIN75_11780"
     CDS             7818..8846
                     /locus_tag="VIN75_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8974892.1"
                     /translation="MRLLATLAAGLMLACAPPAHAAPASFSIAAPDARQVFLAGEMTD
                     WDAGKVAMQRDENGTWHTALDLEPGQWLYKFVVDGQWVQDPATPDHDADGQGGQHSFV
                     FVGDGPWAVAPGTPRGQVQVRQVASRELGATMTVNVYLPPGFRNGQRLPVLWLLHGSS
                     MDADQWFRTGHVERYMDHLIAAGTIRPFVIVMPSAGRDAYDGAGDRFLATELPLWLQS
                     TYGLHATRAQTAVAGMSLGGYGTVALAVRHPQQYGFGVALAGWYPPQLLEQVEHARRL
                     PEKLVLRCGTKDDLLSSNHDLVAVLQKRHAAFDYAEEPGAHTFHFWSTHTALMLTSVD
                     AFFRDLRP"
     gene            complement(8880..>9137)
                     /locus_tag="VIN75_11785"
     CDS             complement(8880..>9137)
                     /locus_tag="VIN75_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8974893.1"
                     /translation="VLSGGEQQRVALARAFVVRPKLLLADEPTGSLDFATGETVMQLM
                     FDLNREIGTTLVLVTHDKTIAARCQRQLHIEAGRLLDTPMA"
BASE COUNT         1396 a         3262 c         3034 g         1445 t
ORIGIN      
        1 cgcggcgtgc gcgcacgtgg cagcgtccgc ctggccgagc gccaccgaca gccccgtcgc
       61 ggccgattgc accagcgtga acgccggccg cgacagcgcc gcgggcgggt cgaaggcgac
      121 cggcgcgcgg ccccagacga cctgcaccag ctccaggatg agatagctca gcccgaacgt
      181 gaccagcagc tccggcacgt ggccgtggcg gtgcacccgg cgcaggcaca ggcgctcgaa
      241 cgccgcgccc agcacgccca cgacgaccgg cgcgacggcc agcgccggcc agaagccgac
      301 gatgcgcgag ctcgcgtagc ccacgtacgc gcccagcatg tagaagccgg catgggcgaa
      361 gttcagcacg cccatcatgc tgaagatcag cgtcaggccc gagctgagca tgaacagcag
      421 cagccccgag ctgaggccgt tgaggatgtt gatgacggtc tggtccacgg tgagggtcag
      481 gccgctcggc cactgctcga tgccgcgtca ccagccaggc ctcggctcgg tcgaggggcg
      541 caccgtggcc cgcgtggatc ctgcgactgc gctttgctgc gcgcacgatg acgacatggc
      601 ggcaagccgc gacgcggccc ggcgcccgac ggccccaagc gcaccgcgac gcgcccgtcc
      661 gacggcccgg agcgcagcgc gaagcgcccg cccgtcatcg cgcgactgcg ctggcgcccg
      721 accgcacggc gcgcgccgcc gcgaagcgcc aacccgtcat cctgcgtgca gtcgcaggat
      781 ccacgcatgc cgatgacgat tccctccgtc accgcctgca ccatcaggac ggccgcttca
      841 tctggcacga ggtgggggtg ctggcgacgt acgcgtcgta gtgcgccacc ggcgcgaacg
      901 tgtagcccgt gttctcgacc gagtacgggt tcttcgcgtc cgtcttctgc cacaccgaga
      961 tccacaggtc ctgctgcaac tggtggtccg tcctgcgcat ctcgatgtcg ccgttgtagc
     1021 tcttcaactt catgccctcg agcgccgcgg ccaccttcac cgggtcggtg gacctggcct
     1081 tggccatcgc ctcggacagc agctgcagct cggtgtagag gtcggcgacg tagaggtcgt
     1141 cgttgaacct ggccttgaag tcgtcctgca gcttgcggat ggggccgctc atgttcgggt
     1201 ggccataccc cacgaagtac acccggccgg cgccggccgc accgatggcg gtgggcgtgc
     1261 cggagttgtt ggcgtagtag gtgaagaact tgacgttcag cccggcctcg ttggccgcct
     1321 tgaccagcag cgacaggtcg gtgccccagt tgccggtgat gacggtatcg gcccccgagg
     1381 ccttgatctt ggcgacgtag ggcgcgaagt cggtgacctt ggccagcggg tgcaggtctt
     1441 cgccgacgat ctcgatgtcg ggacgcttgt gcgcgaggtc ggccttggcg tacttggcca
     1501 cctgcaggcc gtgcgcgtag ttctggccga ggatgtagac cttcctgatg tccttctggt
     1561 ccttgatgaa ggtggtcagg gcctccatct tcatcgaggt gtcggcgtcg agccggaagt
     1621 gccagtagct gcacttgccg ttggtcaggt cggggtcgac ggccgcgtag ttcatgtaca
     1681 gcacttcgtg gcccgggttg cgctcgttgt gcttgttgac ggcgtccacc agcgcgcccg
     1741 ccgcgcccga gccgtcgccc tgggtgatgt agcggatgtc ctggtcgatg gccgacttca
     1801 gcgccgtcag cgattccgcc gggctcacct tgttgtcgaa cggcacgatc tcgaacttca
     1861 cgccggcggg gttgttcttg ttgaacagct cggccaggta ctggaaggtc ttgagctcgt
     1921 tctggccgat gggcgccatc aggccggaca gcgggtcgat ccagccgatc ttgaccgtct
     1981 cgcccttctg cgcgaacgcg gcgccgggat gcagcgcgag gcaggccagc ccgagggtgg
     2041 aagcgaacgc caggggcttg ctcaggaacg tcatggcttt gtctccgtgg tcgccgcgcg
     2101 gctcgcggtc ggggtgacgt tagcccaaca ccgggcggcc gcgctgcagg gactaccccg
     2161 aatgcgcctg tcgcggtcgc gacaagcctt cccggcccgc tcagccggcc gtcggcagca
     2221 cgtggtcgcg gaactgctcg cgcaggcgca gcttctgcaa cttgccggtg gccgtgtgcg
     2281 gcagctcggc gacgacggcc acgtcgtcgg ggatctgcca cttggcgatc ttgccgtcga
     2341 agaagcgcag cagttcctcg cgcgtgacgg tcgcgcccgg cttgggcacc accaccagca
     2401 gcgggcgctc gtcccacttc gggtgcttgc aggcgatgac cgcggcctcg tgcaccgacg
     2461 ggtgcgcgat ggcgatgttc tcgagttcga tcgagctgat ccattcgccg ccggacttga
     2521 tcacgtcctt gctgcggtcg gtgatgaaca tgaagccgtc ggcgtcgatg gccgcgacgt
     2581 cgccggtggg gaaccagccc ggctcgccgt ccacgctgac cagcggcgag tccttggact
     2641 tgaagtagct agagatcacc cacggcccgc gcaccaccag gttgccggcc gccttgccgt
     2701 cccatggcag cgagcggccc tcgtcgtcga tgatggccat gtcgatgccg aagatcacgc
     2761 ggccctggct ctgcagcagg cgttgcttct cctcggccgg cagcgcctcg tgcttggact
     2821 gcagcttcga cagcgtgccc agcggcgaca gctccgtcat gccccaggcg tggatcacct
     2881 cgacgccgta gtcctgctgc aacgacttca tcatcgccgg cgggcaggcc gaaccgccga
     2941 tgaccgtgcg cttgaacgtg ctgaacttca ggtcgttggc cttcatgtag ttcagcaggc
     3001 ccagccagac ggtgggcaca ccggcgctga aggtgacctt ctcggactcg aacaggtcgt
     3061 acagcgatgc gccgtccagg aacggacccg gcatcaccag cttggcgccg accatcgcgg
     3121 cggtgtacgg gatgccccag gcattgacgt ggaacatcgg cacgatgggc aggatgacgt
     3181 cgcgcgtgga gcagttcatc gcgtcgggca gcgccgcggc gtacgagtgc agcacgatgg
     3241 agcgatggct gtagagcgcg cccttcggat tgcccgtggt gcccgaggtg tagcagagcg
     3301 tggcggccgt ctgctcctcg agctcgggcc agtcgtagtc gcccgcgtgc ccggccagca
     3361 ggtcgtcgaa gcacagcagg ttcggcaggc tggtggacgc gggcatctgt ccgcggtggc
     3421 ccatgaggat gaagtggcgc accgtcttga gcttcggcgc cagcttctcg accagcggca
     3481 ggaagcccga gtcgaagcac agcacggcat cctcggcgtc gttggcgatc cactcgatct
     3541 gctccgggaa cagccgcggg ttgatcgtgt ggcagacgcg gccggagccc gagacgccgt
     3601 agtagatctc gacgtggcgg tggccgttcc aggccagcgt ggcgacgcgc tcgcccggcg
     3661 cgacggccag cgcccgcagc gcctgcgcga gctggcgcgc gcgcttctcg acctgcgccc
     3721 agttgctgcg gtggatgtcg ccctcgcagc gcttggacac gatctcgacc tcgcccgcat
     3781 ggcgcgccgc gtgccggatc atcgacgaca cggtcagcgg catgttcatc atctggccca
     3841 tcaggacggt catcgcatgt ctcctcgttg tcgtgtcggc cccgccgcgc tgtcgctcct
     3901 gcgacacccg ccaacagcga agggctgccc tactgtaagg cccttcggcg cccctgcgcc
     3961 agagggctcg ctagactggt tgccaatgtc cgacatcgac tccgcccaag cccccaccgg
     4021 ccccgacgcc gcgcgctgga tcaaccgttt cgcgcgcttc gcgcacgacg tgcccgacgt
     4081 gcgccgcgac gccgaatcca tccccgcgga gacgcgcgcg cgccacccgt tcggcaccga
     4141 cctgcccgcg cagcccttcc ccgagccgca ctgggtcgcc accagcgacg actgcgccgc
     4201 cctgctcggc tggccatcgg actgggccac gcgcgccgac tggcgcgccc tcgacgtgct
     4261 caccggccgc gcgacctggc ccggcctgca cacgatggcc acggtctaca gcggccacca
     4321 gttcggcgtc tgggccggcc agctcggcga cggccgcgcc ctgctgctgg gcgaatggca
     4381 acccgaggcc ggcggcccgt cgttcgagat ccagctcaag ggcgccggcc gcacgccgta
     4441 ctcgcgcatg ggcgacggcc gcgccgtgct gcgctcgtcg atccgcgagt tcctgtgctc
     4501 cgaggcgatg cacgcgctgg gcgttccgac cacgcgcgcc ctgtgcgtgg ccggctcggc
     4561 cctgcccgtg atgcgcgaga cgcccgagac cggcgccgtc gtcacccgcg tcgcgcccag
     4621 cttcctgcgc ttcggccact acgagcactt tgcccacagc ggccagcacg agtcgctgcg
     4681 ccgtctggtc gacttcacga tcgccgcgta cttcccggac ctgcgcgacg gcgccgacgg
     4741 gctcgacccg ctgcacgcct ggctggccga agtcgtccgg cgcacggccg cgacggtcgc
     4801 gcactggcag gcggtcggct tcgcgcacgg cgtgatgaac acggacaaca tgtccatcct
     4861 cggcctgacg atcgactacg gcccgttcgg cttcctcgac ggcttcgacc cgggtcacat
     4921 ctgcaaccac tccgacaccg gcggccgcta cgccttcgca cgccagccca acatcgcctg
     4981 gtggaacctg cacgcgctgg cccacgcgct gcgcccgctg tgcgccgatc ccgacaccac
     5041 gctgccgccc gtgctcgacg ccttcagcga ccgcttcatc gacgcctatt ccgaccacat
     5101 gcgcgccaag ctgggcctgg ttctcgcgca ggacggcgac aaggaactgc tggacgacct
     5161 cctgcagctg atggccggcg agcgcgtcga catgacgatc gcgttccgcg cgttggcgcg
     5221 gttcgaccgc ggcgccggcg cgacgcccgc cgcgttccgc gaccacttca tcgaccgcgc
     5281 cgccgccgac gcctggggcg agcgctacct gcggcgcctg gaggccgagg cgcgccccga
     5341 cgccgagcgc cacgccgcga tggaccgcgc caaccccaag ttcgtgctgc gcaaccacct
     5401 ggcgcaggag gcgatcgcca aggccgaggc gggagacttc ggcgaactgc gccaactgca
     5461 cgacctgctg cgccggccgt acgacgagca gccgggccgc gagcgctatg ccgcgctgcc
     5521 gcccgactgg gcgcgccaca tcgaggtgtc ctgctcgtcc tgaggcgcgc gacgcgcctg
     5581 cgctagactt cgcccccggc acgcaccgct tcccggcgcg cgccgcatca ccgtcccacc
     5641 gcgccgcctc cgcagccggc gtccgcgccc ggccccgcat gccgggcggc ccgccgacca
     5701 tgaaactcct cttcgacatc cttccgctga tcttcttctt cgcgacgtac aagatcgcgg
     5761 gaaggatgcc cgaccagggc gccgcgttcg ccacgcactg gctcggcgcc gtggtgcaag
     5821 gcggcgtggt cggctcgacc gaggcgccgg cgctgctggc cacggccgtc gtgatcgccg
     5881 ccaccctggt gcaggtcagc tggtacaaga tcgcgggacg caagatcgac ctgatgctgt
     5941 gggtcagcac cgcgatgatc atcgtgttcg gcggcctggc cgtctggttc cacaaccaga
     6001 tgttcatcat gtggaagccc agcatctact tctgggtcac cggcctgatc ttctggggca
     6061 gccagacctt cttccacaag aacctgtggc gggcgacgct aggcaacgac atggtcgttc
     6121 ccgacacggt ctggcagcgc ttcaatttcg cctgggtcgc ctttttcgcg ctgatgggcc
     6181 tggtcaatct cctggtcgtt tacttcttcc gcgattactg ggtcggcttc cataccttcg
     6241 gcaccacggc gctgatggcg gtgttcctgc tggtcgcgtt ctattacctc ggcaagcacg
     6301 tcgaacccga actgacggcc gacaagccgg cgccctgacg atggcttcca cccctttgcg
     6361 cgacgcgatg acgcaggccc tgaccgagcg cctggagccc ctggtgctcg aactcgtcga
     6421 cgacagcgcg gcgcacgccg ggcatgccgg cgcgcgtgaa ggcgcgcatt tcaacctgcg
     6481 catcgtgagc gcgaagttcg cgggattgcc ccgtatttcg cgccatcgcc tcgtgtatga
     6541 tgccctccgc ccctggatgg ccgagggcgt tcatgcgctc gccatcgacg cccgcacgcc
     6601 ggaagaagcc tgagccattc gcttccgctt tgtttccgcg tcggagattc tcgatagagt
     6661 ttctcgatgg gtgcgtttcg atgtgtcaag gagtatccgg gtttccgcaa cctgaatttc
     6721 tccggcgaca tggggaaacc cccgggaact tgattgttcc cgttcacgtc caaaaccgtt
     6781 tgctcgaaag tctcccagca tgaatttcaa atcttcgacc gcgctgatcg ccgcgctcgt
     6841 gctgccgctg tccgtgatgg cgcagaacgt cgccaccgtc aacggcaagc cggtgcccat
     6901 gtcgcgtgtc gacgcgctga tcaagaccgc cacgcacggc cagcagcagg acgtgccgcc
     6961 cgaggtcaag gcccaggcca aggaccaggt cgtgctgcgc gagatcttcg cccaggaagc
     7021 cgagaagcag ggcatccccc agtcggccga ctacaaggcg cagctcgaac tcgtgcgcca
     7081 gaccgtgctg atcaacacgc tgttccagaa cttcatcaag acgcacccgg tcagcgacgc
     7141 cgaagccaag gccgagtacg accagatcaa ggccgagcag tcggccctcg agtaccacgc
     7201 gcgccacatc ctggtcgaca gcgaggacga ggccaagaag ctgatcgccc agatcaaggc
     7261 cggctcgaag ttcgaggacg tggccaagaa gagctccaag gacaccgaat cggctgaaaa
     7321 gggcggcgac ctcgactggg ccaagccgag caactacgtg cccgagttcg ccaccgcgat
     7381 gcagggcctg aagaagggcc agatgaccga caccccggtc aagtcccagt tcggctacca
     7441 catcatccgc ctggaagaca cgcgcgccgc gcagttcccg tcgttcgacg aagtcaagga
     7501 caagatcaag cagcagaagg aacaggtcaa gctccaggag taccaggaga agctgcgcaa
     7561 gtccgccaag accgactaca agttcgccgc ggaacagcag cagcaacagc agcaacaggc
     7621 gccggccgct cccgccgccg cgccggcctc cgccgccaag aagtaagacg ccggatccgc
     7681 ggcgcccgcg tcgccgcggc gccccgcgaa agcgccccgc ctgggccagg ccgggcgctt
     7741 ttttcttggc gccggcgtcg accgcgcgac gcgtcgacta gcatggtcgg ccaccctctc
     7801 gtgaccgccc tttcgccatg cgccttctcg cgaccctcgc agccggcctc atgctggcgt
     7861 gcgccccgcc cgcccacgcc gcgccggcca gcttcagcat cgccgccccg gatgccaggc
     7921 aggtcttcct ggctggcgag atgaccgact gggacgcggg caaggtggcc atgcagcgcg
     7981 acgagaacgg cacctggcac accgcgctcg acctcgagcc gggccagtgg ctgtacaagt
     8041 tcgtcgtcga cggccagtgg gtgcaggatc ccgccacgcc cgaccacgac gccgacggcc
     8101 agggcggcca gcactcgttc gtgttcgtcg gcgacgggcc gtgggcggtc gcgcccggca
     8161 cgccgcgcgg gcaggtgcag gtgcgccagg tggcgtcgcg cgagctgggc gcgacgatga
     8221 cggtcaacgt ctacctgccg cccggattca ggaacggcca gcggctgccg gtgctgtggc
     8281 tgctgcacgg cagctcgatg gacgccgacc agtggttccg cacgggtcac gtcgagcgct
     8341 acatggacca cctgatcgcg gcgggcacga tccgaccgtt cgtcatcgtg atgcccagcg
     8401 ccgggcgcga cgcgtacgac ggcgccggcg atcgcttcct cgcgaccgag ctgccgctgt
     8461 ggctgcagtc gacctacggc ctgcacgcga cgcgcgcgca gaccgcggtg gcgggcatgt
     8521 cgctgggcgg ctacggcacc gtggcgctgg cggtgcggca tccacagcag tacggcttcg
     8581 gcgtggcgct ggccggctgg tatccgccgc agctgctcga gcaggtcgag cacgcaaggc
     8641 gcctgcccga gaagctggtg cttcgctgcg gcacgaagga cgacctgctt tccagcaacc
     8701 acgacctggt cgccgtgctg cagaagcgcc acgcggcgtt cgactacgcc gaggaaccgg
     8761 gcgcgcacac cttccatttc tggagcacgc ataccgcgct gatgctgacg tcggtcgacg
     8821 cgtttttccg cgatctccga ccgtgattgc ggatacgggc aaggcgtttc cggagtggct
     8881 cacgccatcg gcgtatccag cagccggccc gcctcgatgt gcagctggcg ctggcagcgc
     8941 gccgcgatcg tcttgtcgtg cgtcaccagc accagcgtcg tgccgatctc gcggttgagg
     9001 tcgaacatca gctgcatcac cgtctcgccg gtggcgaagt cgaggctgcc ggtgggctcg
     9061 tcggccagca gcagcttggg ccggacgacg aaggcacggg cgagcgcgac ccgctgctgt
     9121 tcgccgcccg agagcac
//
LOCUS       DATCRO010000436         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_522180, whole genome shotgun sequence.
ACCESSION   DATCRO010000436 DATCRO010000000
VERSION     DATCRO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522180"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VIN75_11790"
     CDS             <1..459
                     /locus_tag="VIN75_11790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8974894.1"
                     /translation="GMAIAHAKRRLSYSFAVLFIDLDRFKNVNDSLGHSVGDELLIAV
                     ARRLESCLRPGDTVARLGGDEFTVLAEGIATLEQARAIADKIIDAMKPPLETHKAVRL
                     RASIGISLYRPPADASRFLREADRAMYHAKHHHDEVEGVSAFLSDEEFLD"
     gene            495..977
                     /locus_tag="VIN75_11795"
     CDS             495..977
                     /locus_tag="VIN75_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8974895.1"
                     /translation="MSDPNAHPDDPRWKHDGVRVVPADKLDPNTAQTPGMDRQAAITF
                     ARTGAQKLWAGTVHIHPDAKTGAHHHGHLESVIYVVKGRARMRWGERLEFTAEAGPGD
                     FIYVPPFVPHQEINASPTEVLECVLVRSDGQAVAVNLDIEPVEPPEDVAWVDPTHPAG
                     "
     gene            complement(978..1874)
                     /gene="nhaR"
                     /locus_tag="VIN75_11800"
     CDS             complement(978..1874)
                     /gene="nhaR"
                     /locus_tag="VIN75_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEY8974896.1"
                     /translation="MNYKHLHYFWAAAKAGGVVRAGQQLAISPQTLSGQIKLLEDRLG
                     CALFRKAGRGLELTDEGHLAMGYADQIFALGAELEARIGKGSGASSALDFRVGLSDSV
                     PKSIAYRLLEPALSAAANVRLICHEGKLADLLGQLAVQRLDLVLADEPLGRQAGMKAF
                     NHALGSTAMSFFATPELKRSLTGAFPQCLDDAPMLLPGNASAMRQRFDLWLAARKIRP
                     RVIGEFDDAALMKAFGGEGRGVFMTPAVLESETAAQYGVRVVGRSDELVEEFHAISTE
                     RRITHPCVAAITSSARDQFFVE"
     gene            1987..2745
                     /locus_tag="VIN75_11805"
     CDS             1987..2745
                     /locus_tag="VIN75_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8974897.1"
                     /translation="MELLLDPTLWVAFAMLTTLEIVLGVDNIIFLTILVGRLPAARRD
                     AARRLGLAFAMLTRIALLLSLSWVMGLTTELFALAGHAFSGRDLVLLGGGAFLLYKAS
                     REIFDAVEQGDENARVPGKPAAAAAFWSVIAQIAVIDIVFSLDSVITAVGMVDRVGVM
                     IAAVVASVAVMMFAARPIGEFVDRHPSIKVLALAFLAMVGMTLAAEAFGVHVPKAVIY
                     AAIGFSLAVELLNIRARGRRQAARAAELAAPNAS"
     gene            2897..3571
                     /locus_tag="VIN75_11810"
     CDS             2897..3571
                     /locus_tag="VIN75_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY8974898.1"
                     /translation="MYAEDARVVSAGGNARVLRNTYALLALSMLPTVLGAWLGVATGL
                     GAMFSGVTGLVVFMVGAFGLIWAIEKNRNSSAAVPLLLGFTFFMGLMLSRLLGMVLGL
                     ANGTGLVMMAFAGTATIFLGMAGLSSIIKRDLSTMGKWLFVGAVMLMVAGIANVFIQS
                     SALMITMSVMAIGIFSAFVLHDLKRVQDGHETNYVIATLGVYLSLYNVFQNLLVILGV
                     FGGRDE"
     gene            complement(3656..4156)
                     /locus_tag="VIN75_11815"
     CDS             complement(3656..4156)
                     /locus_tag="VIN75_11815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8974899.1"
                     /translation="MDSPKHQLSMTVLMTPDMANFSGNVHGGTILKLLDQVAYACAAR
                     YAGRYVVTLSVDQVMFREPIHVGELVTFLASVNHTGTSSMEVGIKVLAEEIRTQRTRH
                     ANSCFFTMVALGDDRKPVAVPPLEPRTADERRRHQMAELRRGLRSDYRARYDALRQQA
                     DGDDGG"
     gene            complement(4176..4934)
                     /locus_tag="VIN75_11820"
     CDS             complement(4176..4934)
                     /locus_tag="VIN75_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8974900.1"
                     /translation="MSTAFTPDAATAALLAPGGRLRAAINLGNPILAATDPATGRARG
                     VSVDLAQALAERLGVPLDTSEFPAATKSVDAVTGGVADIGFFAIDPLRGAEIAFTDAY
                     ILIEGAYLVRADSRCRANEDVDRAGTTVVVGKGSAYDLFLTRELKAASFLRVPGAAEV
                     VQTLRAGGADVAGGIRQVLVEVAAQDPGLRTLPGRFMVIRQAMGLARTRGPAAEAALR
                     AFVEDVKASGFVAEAMARHAVAGASVAPADTHSN"
BASE COUNT          746 a         1808 c         1788 g          810 t
ORIGIN      
        1 ggcatggcca tcgcccacgc caagcggcgc ctgagctact ctttcgccgt cctcttcatc
       61 gacctcgacc gtttcaagaa cgtcaacgac agcctggggc acagcgtcgg cgacgagctg
      121 ctgatcgcgg tggcccgccg cctcgaatcg tgcctgcggc cgggcgacac cgtggcgcgc
      181 ctcggcggcg acgagttcac cgtgctggcc gagggcatcg ccacgctgga gcaggcgcgc
      241 gcgatcgccg acaagatcat cgacgcgatg aagccgccgc tggagacgca caaggccgtg
      301 cggctgcgcg cgagcatcgg catcagcctg tacaggccgc cggccgacgc ctcgcgcttc
      361 ctgcgcgagg ccgaccgcgc gatgtaccac gccaagcacc accacgacga ggtcgagggc
      421 gtgtcggcat tcctgtcgga cgaggaattc ctcgattgag gcgagaatgg ggcgctcttg
      481 gccggaggtg ccccatgtcc gatccgaacg cccaccccga cgacccgcgc tggaagcacg
      541 acggcgtgcg cgtcgtgccc gccgacaagc tcgatccgaa caccgcgcag acgcccggca
      601 tggatcgcca ggccgccatc acgttcgcgc gcaccggcgc gcagaagctg tgggccggca
      661 ccgtgcacat ccaccccgat gcgaagaccg gcgcccatca ccacggccac ctcgagagcg
      721 tgatctacgt cgtcaaggga cgcgcccgca tgcgctgggg cgagcggctg gagttcaccg
      781 ccgaggccgg tccgggcgac ttcatctacg tgccgccgtt cgtgccgcac caggagatca
      841 acgcgagtcc gaccgaggtg ctcgaatgcg tgctcgtgcg cagcgacggc caggcggtcg
      901 cggtcaacct cgacatcgag ccggtcgagc cgcccgagga cgtggcctgg gtcgatccga
      961 cgcatcccgc gggctgatca ctcgacgaag aactggtccc gcgccgagct cgtgatcgcc
     1021 gccacgcacg gatgcgtgat gcggcgctcg gtggagatcg cgtggaactc ctcgacgagc
     1081 tcgtcgctgc ggccgacgac gcgcacgccg tactgcgcgg cggtctccga ttcgagcacc
     1141 gccggcgtca tgaacacgcc gcggccctcg ccgccgaagg ccttcatcag ggcggcgtcg
     1201 tcgaactcgc cgatgacgcg cggccggatc ttgcgcgcgg ccagccacag gtcgaagcgc
     1261 tggcgcatcg ccgacgcgtt gcccggcagc agcatcggcg cgtcgtccag gcattgcggg
     1321 aacgcgcccg tgagcgagcg cttgagctcc ggcgtcgcga agaagctcat cgcggtggaa
     1381 ccgagcgcgt ggttgaacgc cttcatgccg gcctggcgac ccagcggctc gtcggcgagc
     1441 accaggtcca ggcgctgcac ggcgagctgg cccagcaggt cggccagctt gccctcatgg
     1501 cagatcaggc gcacgttggc ggccgccgac agcgccggct ccagcaggcg gtaggcgatg
     1561 gacttgggca ccgagtcgga caggcccacg cggaagtcga gcgccgacga cgcgccgctg
     1621 cccttgccga tgcgggcctc cagctcggcg ccgagcgcga agatctggtc ggcgtagccc
     1681 atggccaggt gcccctcgtc ggtcagctcg aggccgcgcc cggccttgcg gaacagcgcg
     1741 cagcccagcc ggtcctccag cagcttgatc tgccccgaca aggtctgcgg cgagatggcc
     1801 agctgctgcc ccgcgcgcac gacgccgccg gccttggccg cggcccagaa gtagtgcagg
     1861 tgcttgtagt tcatctcccg gctccgatgc tgcggttttc tcgatgtgac aggtcgctga
     1921 tcacgacttt acgctgaaag tcccggagcc gagaattgcc cgtccgcacc gcaccggaac
     1981 cgcaccatgg aattgctcct cgacccgacc ctgtgggtcg ccttcgcgat gctcacgacc
     2041 ctcgagatcg tgctgggcgt cgacaacatc atcttcctga cgatcctcgt gggccgcctg
     2101 ccggccgccc ggcgcgacgc cgcgcggcgc ctgggcctcg cgttcgcgat gctcacccgc
     2161 atcgcgctgc tgctgtcgct cagctgggtc atgggcctga cgaccgagct gttcgcgctc
     2221 gccggccacg ccttcagcgg ccgcgacctc gtgctgctgg gcggcggcgc gttcctgttg
     2281 tacaaggctt cgcgcgagat cttcgacgcg gtggagcagg gcgacgagaa cgcgcgagtc
     2341 cccgggaaac cggcggccgc cgcggccttc tggagcgtga tcgcccagat cgccgtcatc
     2401 gacatcgtgt tctcgctgga ctccgtgatc accgccgtgg gcatggtcga ccgcgtgggc
     2461 gtgatgatcg ccgcggtggt cgcctcggtg gcggtcatga tgttcgcggc gcgtccgatc
     2521 ggcgagttcg tcgaccgcca cccgtcgatc aaggtgctgg cactggcctt cctggcgatg
     2581 gtgggcatga cgctggccgc cgaggccttc ggcgtgcacg tgcccaaggc cgtgatctac
     2641 gcggcgatcg gcttctcgct ggccgtggag ctcctgaaca tccgcgcccg tggccggcgc
     2701 caggccgcgc gggcggccga gctggccgcg ccgaacgcct catgaacgtt caacgatacg
     2761 atttatgcga tgcattgttt cgattcattc gcatttactc gcatcgtcga tcgagcgaca
     2821 gtgagctgtc cccaatgcaa ggagtcttgc cgtgaatgtg aaccttcaac ccagttccga
     2881 ccgcccgcgc cgcccgatgt acgcggaaga cgcccgcgtc gtctcggccg gaggcaatgc
     2941 gcgcgtgctg cgcaacacct acgcgctgct ggcgctgtcc atgctgccca ccgtgctggg
     3001 cgcctggctg ggcgtggcca cgggcctggg cgccatgttc tccggcgtca ccggcctggt
     3061 cgtgttcatg gtcggcgcgt tcggcctgat ctgggccatc gagaagaacc gcaattcgtc
     3121 ggccgcggtg cccttgctgc tgggcttcac gttcttcatg ggcctgatgc tgtcgcgcct
     3181 gctgggcatg gtgctgggcc tggccaacgg caccggcctc gtgatgatgg cgttcgccgg
     3241 caccgccacg atcttcctgg gcatggccgg cctgtcctcc atcatcaagc gcgacctgtc
     3301 gacgatgggc aagtggctgt tcgtcggcgc cgtgatgctg atggtggcgg gcatcgccaa
     3361 cgtcttcatc cagtcgagcg cgctgatgat cacgatgtcc gtgatggcga tcggcatctt
     3421 ctcggccttc gtgctgcatg acctcaagcg cgtgcaggac ggccatgaga cgaactacgt
     3481 catcgcgacg ctgggcgtgt acctgagcct gtacaacgtg ttccagaacc tgctggtcat
     3541 cctgggcgtg ttcggtggcc gcgacgagtg attcgaactc gttgactcga ccctgctgcc
     3601 cccgcgggtc cacccggcgg gggctcgtgc gtccaggcgt cggacgcacc gggcgtcagc
     3661 cgccgtcgtc gccgtcggcc tgctggcgca gcgcgtcgta gcgggcgcga tagtccgagc
     3721 gcaagccgcg gcgcagctcg gccatctggt ggcgccggcg ctcgtcggcc gtcctgggct
     3781 ccagcggggg cacggccacg ggcttgcggt cgtcgccgag cgccaccatc gtgaagaagc
     3841 agctgttggc gtggcgcgtg cgctgggtgc ggatctcctc ggccagcacc ttgatgccca
     3901 cctccatcga gctggtgccg gtatggttga ccgaggcgag gaaggtgacg agctcgccga
     3961 cgtggatcgg ctcgcggaac atcacctggt cgaccgacag cgttacgacg tagcgacccg
     4021 cgtagcgcgc cgcgcacgca taggcgacct ggtccagcag cttcaggatc gtgccgccgt
     4081 gcacgttgcc cgagaagttg gccatgtccg gcgtcatcag cacggtcatc gagagttgat
     4141 gcttgggcga gtccatgtcg cgtccttggc gaagttcagt tcgaatgcgt gtcggcgggg
     4201 gcgacgctgg cgccggccac cgcatgccgg gccatggcct cggcgacgaa gccgctggcc
     4261 ttgacgtcct cgacgaaggc gcgcaacgcg gcctcggccg cgggcccgcg cgtgcgcgcg
     4321 aggcccatcg cctggcggat caccatgaag cggccgggca gggtgcgcag gccggggtcc
     4381 tgggccgcca cctccaccag gacctgccgg atgccgccgg ccacgtcggc gcctccggcg
     4441 cgcagcgtct gcacgacctc cgcggcgccg ggcacgcgca ggaacgacgc cgccttcaac
     4501 tcacgcgtga ggaacaggtc gtacgcgctg cccttgccga cgacgacggt cgtgccggcg
     4561 cgatcgacgt cctcgttggc gcggcagcgc gaatcggcgc gcaccaggta cgcgccttcg
     4621 atcaggatgt aggcgtcggt gaacgcgatc tcggcgccgc gcagcgggtc gatcgcgaag
     4681 aaaccgatgt cggccacgcc gccggtcacc gcgtcgaccg acttcgtcgc cgcggggaac
     4741 tcgctcgtgt ccagcggcac gcccaggcgc tcggccagcg cctgcgccag gtcgaccgag
     4801 accccgcggg cgcgtccggt ggcggggtcg gtggccgcga ggatcgggtt gcccaggttg
     4861 atggccgcgc gcaggcggcc gccgggcgcg agcagggcgg cagtggccgc gtcgggggtg
     4921 aaggcggtgc tcatcgggcc attctgctgg aaggcgccgc cggcgctgtc tgcccggcgc
     4981 cacatccgcc tcggctcgtc atccgtattg aacctcggac gagcccgagg gtcatacacg
     5041 acagcgccgg gcgactgcgc gccggacgtt gcaagatccg cgctgccgcg ctgtaaggat
     5101 cccggcgccc gcgccgactg actcgcatca tcgccacgaa acgaagaaag ac
//
LOCUS       DATCRO010000437         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_421300, whole genome shotgun sequence.
ACCESSION   DATCRO010000437 DATCRO010000000
VERSION     DATCRO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421300"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VIN75_11825"
     CDS             <1..811
                     /locus_tag="VIN75_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8974901.1"
                     /translation="LVVAGRHIRGLAFLDVLLGDTPALSMSERFYQRAMVGDHEEIVA
                     AAHQYLKRKSFARYCDKILIPALQLAAADLGKHSITPEQQATVKSTVAHVIEALGADA
                     CRPTWRNRRGSVLDDTSIGAHLRHQRERAMGKWQGPLVVPPGSVALCIGLGSMRDDLV
                     TELLVRILRTQDIDARHLSLDDLVQEPPPGASPASIAMLFVVSAYPADEWDRIADRLP
                     ALRARFVDAVFVGLLSDGEFAPADEAMDVVVRSFEEASAEAAERYGKKPAA"
     gene            985..1635
                     /locus_tag="VIN75_11830"
     CDS             985..1635
                     /locus_tag="VIN75_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8974902.1"
                     /translation="MSTRKSGPLDYNVLPEVLRPLAERSEIRRFRKGALVIDEGDRGD
                     ALLIILAGELRAFTGSDTGRHVTYALYGPGDYLGEMGLDGGTRMASVEALSDTTCAYV
                     ERGTLLQYFAERPQFAFEIIGKVIQRARVATLAANQYAMNEAYLRLKRWLDAVAQAQF
                     DGTRLVREPLAEIDLPQRLGCSQEMAGSILRDLEHAAIISLEDGRLRILRELPSHW"
     gene            complement(1759..>2668)
                     /gene="ilvA"
                     /locus_tag="VIN75_11835"
     CDS             complement(1759..>2668)
                     /gene="ilvA"
                     /locus_tag="VIN75_11835"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY8974903.1"
                     /translation="PDAMIQSVRKGRRVTLADVGLFSDGTAVKLVGAETFRVARALVD
                     EYIAVDTDAACAAIKDVFQDTRSILEPAGALGVAAIKQYVETHKLKGKTFVAVTCGAN
                     MNFDRLRFVAERAEFGEQREALFAVTVPEERGSFRRFCALIGPPGSAPRQVTEFNYRI
                     SHANEAHVFVGISIARRDEAEKIARHFEKHGFPTANLTGDELAKEHVRHMVGGRSTLA
                     AGERLFRFEFPERPGALMRFLDSMHPDWNITLFHYRNQGADYGRILVGLVVPPKDDKA
                     LATFLSTLAYPWVEETDNPVYRLFLR"
BASE COUNT          391 a          952 c          934 g          391 t
ORIGIN      
        1 cctggtggtg gccggccgcc acatcagggg cctggccttc ctcgacgtgc tgctgggcga
       61 cacgccggcg ctgtcgatgt ccgagcgctt ctaccagcgc gcgatggtgg gcgaccacga
      121 ggagatcgtg gccgcggcgc accagtacct caagcgcaag tcgttcgcgc gctattgcga
      181 caagatcctg atccccgcgc tgcagctggc ggcggccgac ctgggcaagc acagcatcac
      241 gccggagcag caggccaccg tcaagagcac ggtggcgcac gtcatcgagg cgctgggcgc
      301 ggacgcctgc cgcccgacgt ggcgcaaccg gcgcggctcg gtgctcgacg acaccagcat
      361 cggcgcgcac ctgcgccacc agcgcgagcg cgcgatgggc aagtggcagg ggccgctggt
      421 ggtgccgccc ggctcggtgg cgctgtgcat cggcctgggc tcgatgcgcg acgacctcgt
      481 caccgaactg ctcgtgcgca tcctgcggac gcaggacatc gacgcgcgcc acctgtcgct
      541 cgacgatctc gtccaggagc cgccgccagg cgcgagcccg gccagcatcg cgatgctgtt
      601 cgtcgtcagc gcgtacccgg ccgacgaatg ggaccgcatc gccgaccggc tgcccgcgct
      661 gcgcgcgcgt ttcgtcgatg ccgtgttcgt gggcctgctg tcggacggtg aattcgcgcc
      721 tgccgacgag gcgatggacg tcgtcgtccg ctcgttcgag gaggcctccg ccgaggcggc
      781 cgagcgctac gggaaaaagc ctgcggcctg aacgcgggca gccggtcgtc gtacctctcc
      841 tgccgactgg cgcggatgtg caaaacgtcc gcccgcgcga tgtcgacagc gcgttcaagg
      901 ctgtcccgga cgtggccaaa ccggcgatac tgaagcgacg aacccgcgcc gcgcgcgccg
      961 cgacgagccc cagagaaccc gagcatgtcc acccgcaaat ccggccccct cgactacaac
     1021 gtcctgcccg aagtcctgcg tccgctggcc gaacgcagcg agatccggcg cttccgcaag
     1081 ggcgcgctcg tgatcgacga gggcgaccgc ggtgacgcgc tgctgatcat cctggccggc
     1141 gagctgcgcg ccttcacggg ctcggacacc ggccgccacg tcacctacgc gctgtacggg
     1201 ccgggcgact acctcggcga gatgggcctg gacggcggca cccgcatggc cagcgtcgag
     1261 gcgctgtccg acaccacctg cgcctacgtc gagcgcggca cgctgctgca gtacttcgcc
     1321 gagcgtccgc agttcgcctt cgagatcatc ggcaaggtga tccagcgcgc ccgcgtcgcc
     1381 acgctggccg ccaaccagta cgccatgaac gaggcctacc tgcgcctgaa gcgctggctc
     1441 gacgcggtcg cgcaggccca gttcgacggc acgcgcctcg tgcgcgagcc gctggccgag
     1501 atcgacctgc cgcagcgtct cgggtgctcg caggagatgg ccggcagcat cctgcgcgac
     1561 ctggagcatg cggcgatcat ctcgctggag gacgggcgct tgcgcatcct gcgcgagctg
     1621 ccgagtcatt ggtagccagc acccggccga ggcgcatcct ggcgcctgcc gccgcccgtc
     1681 atcctgcgac tgcgcgcagg atgacgcttg gcgggcgcgg agcggcgtcg cttccgcagg
     1741 cgtcgagacg cccgcagctc agcgcaggaa caacctgtag accgggttgt ccgtctcctc
     1801 cacccacgga tacgccagcg tggacaggaa cgtcgccagc gccttgtcgt ccttgggcgg
     1861 caccaccagg cctaccagga tgcggccata gtcggcgccc tggttgcggt agtggaacag
     1921 ggtgatgttc cagtcggggt gcatcgagtc gaggaagcgc atcagcgcgc cggggcgctc
     1981 ggggaactcg aagcggaaca ggcgctcgcc cgcggccagc gtgctgcggc cgccgaccat
     2041 gtggcgcacg tgctccttcg cgagctcgtc gccggtgagg ttggccgtcg ggaagccgtg
     2101 cttctcgaag tgcctggcga tcttctcggc ctcgtcgcgc ctggcgatcg atatgccgac
     2161 gaacacgtgc gcctcgttcg cgtgcgagat gcggtagttg aactcggtga cctggcgcgg
     2221 cgcgctgccc ggcggcccga tcagcgcgca gaagcggcgg aagctgccgc gttcctcggg
     2281 caccgtcacc gcgaacagcg cctcgcgctg ctcgccgaac tcggcgcgct cggcgacgaa
     2341 gcgcaggcgg tcgaagttca tgttggcgcc gcaggtgacg gccacgaagg tcttgccctt
     2401 gagcttgtgc gtctcgacgt actgcttgat cgcggccacg cccagcgctc ccgcgggctc
     2461 gaggatgctg cgggtgtcct ggaacacgtc cttgatggcg gcgcaggcgg cgtcggtgtc
     2521 gacggcgatg tactcgtcga ccagcgcgcg cgcgacgcgg aacgtctcgg cgccgaccag
     2581 cttgacggcc gtgccgtccg agaacagccc cacgtcggcc agcgtgacgc gccggccctt
     2641 cctgaccgac tggatcatcg cgtccgga
//
LOCUS       DATCRO010000438        12182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_814739, whole genome shotgun sequence.
ACCESSION   DATCRO010000438 DATCRO010000000
VERSION     DATCRO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814739"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VIN75_11840"
     CDS             complement(<1..394)
                     /locus_tag="VIN75_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974904.1"
                     /translation="MTSSSPLTPRPHDAVPGHDAKSGVGAVPHAAAVHPGERHLIERG
                     YVPVRQLTGKVIGVWGDAHIRLHDGEVRPLRVGDVVRKGDVVLTSQDGIVQIEGSHAK
                     AMLAAGGDDVEKIISQVGNGDADVVPAAG"
     gene            complement(711..2237)
                     /locus_tag="VIN75_11845"
     CDS             complement(711..2237)
                     /locus_tag="VIN75_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY8974905.1"
                     /translation="MSVEQDNIAAPGVAKAKAPSPPAPAKPAPPLTVTATPAPPAAAA
                     AAAKPTLRQSLRAWRIRFDAWLAGAPLQPGEIPGMAVFEREAVEVMSRQHTLRARKIT
                     RIAVFVVAALLVWSAFARVDEVTRGDGRVVPSRQLQVLQSLDGGVVEQILVHEGDKVE
                     AGQVLLMIDPTRATSAVADSAAQGFALEARVARLRALAQGTSFLPPTPSTDEERALVE
                     GEKKLYDSRVTELQTQVQIVHQQLSQHEQELAEVQARKAAAQRALELSTQELNQTRPL
                     LATGAVSQVDILRLERDVTKNKGDMEQAQAQIGRAQAAIQESSRKIQETELAFRNEAG
                     RDLAEALGKLNALNQGQAGLADKVSKTQVKSPVRGRVQRLLANTVGGVVQPGKDIVEI
                     VPLDDSLVLDARVQPRDIAFIHPGQKANVKFTAYDFSIYGGLDADVENISPDSVVDDK
                     GNAYYVVRVRTHLTGFNEKLPIIPGMTAEVDILTGQKTVLSYLLKPVLKVKSYALRER
                     "
     gene            complement(2234..4453)
                     /locus_tag="VIN75_11850"
     CDS             complement(2234..4453)
                     /locus_tag="VIN75_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY8974906.1"
                     /translation="MSVPSSAVPPATPAQPPAGPRAAPASVAGGAVRQDLVHPDPLLD
                     CLVEVCRLHGQHATRASLSAGLPLAHGRLTLNLAERAAARAGMATKLQRVAVDDIDAA
                     TLPVILLLSGDKACVLLGREQGRHRVLLPETGQGTVLLDDGELAARATGLALYVRPHF
                     RFDKRTEGVAQPTTGHWFWSALLAQRLVYKDILWAAALINVFALALPLFSMNVYDRVV
                     PNAAFETLWALSIGVLLILGSDLLMRKLRSRFVDEASARIDVRISATLLERVLGMRLE
                     NRPESVGSFSQNLRGFEQVRDVITSTTVTALIDLPFALLFLVVIVWISPWLMIPVVLA
                     FGGVLAYGWIVQKKLHALAETTYKASAVRNSTLVECLTGIETIKAQGAESLIQARWER
                     TNVHLAATNVKMRSLSSGSIYVTSTITQLMSVVLVIVGVYLIGDKSLTMGALVAVNML
                     AGRAMAPAGAIVGLLMQYQGARTALEGLEKIMRTAVERPQDATFVERRDLEGAIEFRN
                     VAFHYPNRRENALEGVSFKIRPGEKVALIGKVGSGKSTIQRLAMGLFQPTAGAVLVDG
                     IDLRQVDPADLRRNMGYVSQDVLLFYGSLRENIAFGMPYAEDGAIVAAAEVAGLTETV
                     NRHPAGFDMPVGERGESLSGGQRQSVGIARAVLHNAPMLLLDEPTSAMDFSSEAQITQ
                     RLMAFASNKTVVLVTHRTSLLALVERVIVIDGGKVVADGPRDRIMEALQSGRIARAQ"
     gene            complement(4494..5039)
                     /locus_tag="VIN75_11855"
     CDS             complement(4494..5039)
                     /locus_tag="VIN75_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8974907.1"
                     /translation="MFSPRSIRHCRACGAPTAYREPPEDNRERAICSSCGTIHYENPL
                     NVVGTVPVWEGKVLLCLRNIEPRKGFWTLPAGFMELNETTEQGALRETEEESGAKVRM
                     GPLFTLLNVPHVGQVHLYYRAEMTGPGLDPGPETIEARLFDEAEIPWDSLAFKTVKVT
                     LEHFFADRRLGQFTVHCADIA"
     gene            5142..5846
                     /locus_tag="VIN75_11860"
     CDS             5142..5846
                     /locus_tag="VIN75_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8974908.1"
                     /translation="MTDVITVPVVPRVPVRGGGQFPVHRIYCVGRNYAEHAAEMGHDA
                     RESPFFFMKPADVVLPVRAGETGFMDYPSLTSDLHHEVELVVAIGDAAAGNGRDVAEE
                     DAPRLIFGYAVGLDMTRRDLQAVAKKAGRPWSIAKGFDQSAPIGEIVRIEETGELGAG
                     AITLDVDGAPRQKGDLSDLIWNVRETISWLSRAWTLKPGDLIFTGTPAGVAAVGRGQT
                     LTGRIAGLGELVVTLR"
     gene            5857..6492
                     /gene="maiA"
                     /locus_tag="VIN75_11865"
     CDS             5857..6492
                     /gene="maiA"
                     /locus_tag="VIN75_11865"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY8974909.1"
                     /translation="MELYSYFRSSASWRVRIALALKGLDVDYRAVHLLRKEQSAPDYV
                     QKSTSGLVPLLVTDDGLRLHQSMAIIEYLDETVPEPPLLPRDAAGRARVRALAQDIAC
                     EIHPLNNTRVLGYLTNTLGVGDEAKSAWIRRWVEGGLEVVERQLAARPATFCDGEAPG
                     LADCVLVPQIFNAQRFKCELGHVPNVMRVHAACMALPAFAGTQPSACPDAE"
     gene            6499..7293
                     /gene="pgeF"
                     /locus_tag="VIN75_11870"
     CDS             6499..7293
                     /gene="pgeF"
                     /locus_tag="VIN75_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY8974910.1"
                     /translation="MPLEIVDPLPLDAFFRPVSWPRGTGALMTTRKGGVSAAPFDSLN
                     LKAQSADPLDQIAVAENRRRFAATLGAAPVFLDQVHGTRVVRLATSHLGPEAPIEQAD
                     ASITTVPGIACTVMVADCLPVLFATRDGRAVGAAHAGWRGLAAGVLEATVDAIRAAVP
                     VPASDIVAWLGASIGPTAFEVGADVPAAFHEGEARHFVPAPARDGVPKWHADLPALAE
                     GRLRRLGLTEVSGCGECTVSDASRFFSYRRDGVTGRLAAAAWIDVA"
     gene            complement(7219..7482)
                     /locus_tag="VIN75_11875"
     CDS             complement(7219..7482)
                     /locus_tag="VIN75_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974911.1"
                     /translation="MLIVAAGWIYVVLMMVLAEALGPGGSVPGAIVTLFLYGALPLGI
                     VLYIGNARARAKARRAAGASGDVDPGRGGQAAGDAVAPVREEA"
     gene            7683..9521
                     /gene="phaC"
                     /locus_tag="VIN75_11880"
     CDS             7683..9521
                     /gene="phaC"
                     /locus_tag="VIN75_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY8974912.1"
                     /translation="MARSAPTKTAARTAARPARRAPRARAAAAPSDKAVEAGIARATE
                     RAGAAPRATTQAHGPEAFGAAVGGFLSSLSGLQVPQATLSQLQEQYVNHATSLWNGAV
                     DRMPLFGTQDKPAEAAVIGDRRFSAEDWTKNPAAAFTAQMYLLNARTLTQLADSLQGD
                     EKTKARVRFAVQQFVDAASPANYLALNPEAQRKAIETQGESLAQGLQQLWGDVQQGHL
                     SQTDESVFEVGRNVATTEGSVVFENDYLQLIEYKPLTPRVHARPMLFVPPCINKYYIL
                     DLQPSNSLIRHTVAEGHRTFVISWRNPDESMQDRTWDDYIENAVIRSIEVVREITGAR
                     TINTLGFCVGGTLLATGLGVLAARGQHPAESLTLLTTFLDFSNTGVLDIFVDEASVQL
                     REMTLGAQAPGGPKLLKGGELASTFSFLRPNDLVWNYVVGNYLKGDKPPAFDLLYWNS
                     DATNMPGPMYCWYLRNTYLENALREPGALTVCGEKLDLSQIKAPAFIYGSREDHIVPW
                     KGAWESTKILGGKTRFVLGASGHIAGVINPPKAGKRSHWTNDKPAVSADAWLAGATEH
                     PGSWWTGWSQWLAGFGGGDAPAPKGPGGHGYKAIEPAPGRYVQQKA"
     gene            9591..10775
                     /locus_tag="VIN75_11885"
     CDS             9591..10775
                     /locus_tag="VIN75_11885"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8974913.1"
                     /translation="MTQDIVIVAAARTAVGKFGGSLAKIPASDLGATVLKALLERARL
                     TGEQVSEVILGQVLQAGTGQNPARQAVIKGGLGNGVPAMTINKVCGSGLKAVMLAAQA
                     IRDGDADIVLAGGQESMSLAPHVLPNSRDGQRMGDWKLVDTMINDGLWDVYNQYHMGI
                     TAENVAKKYGISREQQDALAFASQQKAAAAQDAGRFKDEIVPVAIPQKKGDPILFDAD
                     EFINRKSTAEALAGLRPAFDKAGSVTAGNASGLNDGAAGVVVMSAARADALGLKPLAR
                     IASYASAGLDPAYMGMGPVPAARKALERAGWKPADLDLLEINEAFAAQACAVHQEMGW
                     DTSKVNVNGGAIAIGHPIGASGARILVTLLHEMQRRNARKGIASLCIGGGMGVALTVE
                     RP"
     gene            10875..11612
                     /gene="phbB"
                     /locus_tag="VIN75_11890"
     CDS             10875..11612
                     /gene="phbB"
                     /locus_tag="VIN75_11890"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY8974914.1"
                     /translation="MSQKVAYVTGGMGGIGTAICQRLHRDGFKVIAGCGPSRDHAKWI
                     AEQKALGYTFHASVGNVGDWDSTVAAFQKVKADLGPIDVLVNNAGITRDGMFRKMTRA
                     DWDAVIDTNLTSLFNVTKQVVADMVEKGWGRIIQISSVNGAKGQAGQTNYSAAKAGMH
                     GFSMALALEMANKGVTVNTVSPGYIGTDMVMAIKPEVLEKIVATIPVKRLGTPDEIAS
                     IVGWLAGPESGFTTGAEFAVNGGLHMG"
     gene            11695..11844
                     /locus_tag="VIN75_11895"
     CDS             11695..11844
                     /locus_tag="VIN75_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974915.1"
                     /translation="MTKTLREETMDDMPQPAGRGGAPSRRRELTMTAAASHRPGIDQP
                     RGMFR"
     gene            11841..>12182
                     /locus_tag="VIN75_11900"
     CDS             11841..>12182
                     /locus_tag="VIN75_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974916.1"
                     /translation="MKLPLVIAIVVALGLALAAWFFRGSPMFAGAARAIGVRHENATP
                     DAAATFKAAGVHKCVGAHGTSYIDGPCPAGMREAAASGGTVTVTSFPKPAPAAASSAL
                     GGPIVKPMDPDE"
BASE COUNT         1867 a         4323 c         4240 g         1752 t
ORIGIN      
        1 ggccggcggc cggcacgacg tcggcgtcgc cgttgcccac ctgggagatg atcttttcga
       61 cgtcgtcgcc gccggcggcc agcatcgcct tggcgtgcga gccttcgatc tggacgatgc
      121 cgtcctgcga ggtgagcacc acgtcgccct tgcgcaccac gtcgcccacg cgcagcggcc
      181 ggacttcgcc gtcgtgcagc cggatgtgcg cgtcgcccca gacgccgatc accttgcccg
      241 tgagctggcg caccggcacg tagccgcgtt cgatcaggtg gcgctcgccc ggatgcaccg
      301 cggcggcatg cggcacggcg ccgaccccgg acttcgcgtc gtggccggga acggcatcgt
      361 gcgggcgggg ggtcagcggg gaggacgacg tcatgcctgg atactccgaa ggaagccgga
      421 ttaccggcgc cggctcgtcg tcgtatcggc cattccaccg aatagttcag cagaaaattc
      481 tgggcgcacg acggccgcac ggtacctgta actgttccgt cggattatcc cggcaacgcc
      541 ggattcttat aaggcgcaat gaggccgatt tccgtgatcg cggtcacgga tcgctgtcca
      601 ggacggccaa tagcgagaat gccctacagg cgtgaaaaag taacggaact cgcgcgcccg
      661 aatttccagt ccggaagcag acggccggat gccaactagt tggcaatatc tcagcgctcc
      721 cgcagggcgt atgacttgac tttgagcacg ggcttcaaca aatacgagag caccgttttc
      781 tggccggtca ggatatccac ctcggccgtc atgcccggga tgatgggcag cttctcgttg
      841 aagccggtca ggtgggtgcg cacgcgcacg acgtagtacg cgttgccctt gtcgtcgacg
      901 acgctgtccg ggctgatgtt ctcgacgtcg gcgtccaggc cgccgtagat cgagaagtcg
      961 taggccgtga acttcacgtt cgccttctgg cccgggtgga tgaaggcgat gtcgcgcggc
     1021 tggacgcgag cgtcgagcac cagcgagtcg tccagcggca cgatctcgac gatgtccttg
     1081 cccggctgca ccacgccgcc cacggtgttg gccagcaggc gctgcacgcg gccgcgcacc
     1141 ggcgacttga cctgtgtctt gctgaccttg tcggccaggc ccgcctggcc ctggttcagc
     1201 gcgttgagct tgcccagcgc ctcggccagg tcgcggccgg cctcgttgcg gaaggccagc
     1261 tccgtctcct ggatcttgcg gctggactcc tggatggcgg cctgcgcccg cccgatctgg
     1321 gcctgcgcct gttccatgtc gcccttgttc ttcgtgacgt cgcgctccag ccggaggatg
     1381 tcgacctgcg agacggcacc ggtggccagc agcggccgcg tctggttcag ctcctgggtc
     1441 gacagctcca gcgcgcgctg ggcggcggcc ttgcgggcct gcacctcggc cagctcctgc
     1501 tcgtgctgcg acagctgctg gtgcacgatc tgcacctggg tctgcagctc ggtcacgcgc
     1561 gagtcgtaga gcttcttctc gccctcgacc agcgcgcgct cctcgtcggt ggacggcgtg
     1621 ggcggcagga agctggtgcc ctgcgcgagc gcgcgcagcc gcgccacgcg cgcctcgagc
     1681 gcgaagccct gggcggcgct gtcggccacg gcggacgtgg cgcgcgtcgg gtcgatcatc
     1741 agcagcacct ggccggcctc gaccttgtcg ccctcgtgca ccaggatctg ttcgacgacg
     1801 ccgccgtcca gcgactgcag cacctgcagc tgcctggacg gcacgacgcg cccgtcgccg
     1861 cgcgtgacct cgtcgacgcg cgcgaacgcg gaccagacca gcagcgcagc gaccacgaac
     1921 accgcgatgc gcgtgatctt gcgggcgcgc agcgtgtgct ggcgcgacat cacctcgacg
     1981 gcctcgcgct cgaacacggc catgccgggg atctcgcccg gctgcagcgg ggcgccggcc
     2041 agccaggcgt cgaagcggat gcgccaggcg cgcagcgact gccgcagcgt gggtttggcc
     2101 gcagccgccg ccgcggcggg aggggcgggc gtcgccgtga cggtcaacgg cggcgccggc
     2161 ttggcgggcg cgggcggcga cggcgccttc gccttggcca cgccgggggc ggcaatgttg
     2221 tcctgttcca cgctcattgc gccctcgcga tgcgcccgct ctgcagcgct tccatgatgc
     2281 ggtcgcgcgg gccgtcggcc acgaccttgc cgccgtcgat gacgatcacg cgctcgacca
     2341 gcgccagcag cgacgtgcgg tgcgtcacca gcaccacggt cttgttgctg gcgaacgcca
     2401 tcaggcgctg ggtgatctgc gcctcggacg agaagtccat cgcgctggtg ggctcgtcca
     2461 gcagcagcat cggcgcgttg tgcagcaccg cgcgcgcgat gcccacgctc tggcgctggc
     2521 cgcccgagag cgactcgccg cgctcgccga ccggcatgtc gaagccggcg ggatggcggt
     2581 tgaccgtctc ggtcaggccc gccacctcgg cggccgccac gatggcgccg tcctcggcgt
     2641 agggcatgcc gaaggcgatg ttctcgcgca ggctgccgta gaacagcagc acgtcctgcg
     2701 acacgtagcc catgttgcgg cgcaggtcgg ccgggtcgac ctggcgcagg tcgatgccgt
     2761 ccaccagcac ggcgccggcc gtcggctgga acaggcccat cgccaggcgc tggatggtgg
     2821 acttgccgga accgaccttg ccgatcagcg ccaccttctc gcccggcctg atcttgaagc
     2881 tgacgccctc cagcgcgttc tcgcgccggt tcgggtagtg gaaggcgacg ttgcggaact
     2941 cgatggcgcc ctcgaggtcg cgccgttcga cgaacgtcgc gtcctgcggg cgctcgacgg
     3001 cggtgcgcat gatcttctcg aggccctcga gcgcggtgcg cgcgccctgg tactgcatca
     3061 gcaggcccac gatggcgccg gcgggggcca tcgcgcggcc ggccagcatg ttgacggcca
     3121 cgagcgcgcc catcgtcagc gacttgtcgc cgatcaggta cacgcccacg atcaccagca
     3181 ccacgctcat gagctgcgtg atcgtggagg tgacgtagat cgagcccgag gacaggctgc
     3241 gcatcttcac gttggtggcg gccaggtgca cgttggtgcg ctcccagcgc gcctggatca
     3301 ggctctcggc gccctgggcc ttgatggtct cgatgccggt gaggcactcg accagcgtcg
     3361 agttgcgcac ggcgctggcc ttgtaggtgg tctcggcgag cgcgtgcagc ttcttctgca
     3421 cgatccagcc atacgcgagc acgccgccga acgccagcac caccgggatc atcagccacg
     3481 gcgagatcca cacgatcacg acgaggaaca gcagagcgaa cggcaggtcg atcagcgcgg
     3541 tgacggtggt ggacgtgatg acgtcgcgca cctgctcgaa gccgcgcagg ttctgcgaga
     3601 acgagccgac cgactcgggc cggttctcca gccgcatgcc cagcacgcgc tccagcagcg
     3661 tggcggagat cctgacgtcg atgcgcgcgc tggcctcgtc gacgaagcgg ctgcgcagct
     3721 tgcgcatcag caggtccgag cccaggatca gcagcacgcc gatggacagc gcccacagcg
     3781 tctcgaacgc ggcgttgggc acgacgcggt cgtacacgtt catcgagaac agcggcagcg
     3841 ccagcgcgaa cacgttgatc agcgcggcgg cccagaggat gtccttgtag accaggcgct
     3901 gggcgagcag cgcgctccag aaccaatggc ccgtcgtggg ctgggcgacg ccctcggtgc
     3961 gcttgtcgaa gcggaaatgc gggcgcacgt acagcgccag gccggtggcg cgcgcggcga
     4021 gctcgccgtc gtccagcagc acggtgccct ggcccgtctc gggcaacagc acgcggtggc
     4081 ggccctgctc gcggccgagc agcacgcagg ccttgtcgcc gctcagcagc aggatgacgg
     4141 gcagggtggc ggcgtcgatg tcgtcgaccg ccacgcgctg cagcttggtg gccatgcccg
     4201 cgcgcgcggc ggcgcgttcg gccaggttca gcgtcaggcg gccatgggcg agcggcaacc
     4261 ccgccgacag cgacgcgcgc gtggcgtgct ggccatgcag gcggcagacc tcgaccaggc
     4321 agtcgagcag cggatccgga tggacgagat cctggcggac ggctcccccc gccacggacg
     4381 caggcgccgc cctgggcccg gccggcggct gggcgggcgt tgcgggcggg acggccgagg
     4441 agggaacgct catctggggt tggtctcgtt gaggacaccc accgggcgtc ccgtcatgcg
     4501 atatcggcgc agtgcaccgt gaattgaccc aggcgccggt cggcgaagaa gtgctccagc
     4561 gtcaccttca cggtcttgaa cgccagcgag tcccacggga tctcggcttc gtcgaacagc
     4621 cgggcctcga tcgtctcggg gcccggatcc agcccggggc cggtcatctc ggcgcgatag
     4681 tagaggtgga cctggcccac gtggggcacg ttcagcagcg tgaacagcgg tcccatgcgc
     4741 acttttgcgc cggattcctc ttcggtctcg cgcagggcgc cttgttccgt ggtctcgttc
     4801 aattccatga aaccggccgg gagtgtccag aaacccttac gcggctcgat gttgcgcagg
     4861 cacagcagca ccttgccttc ccacaccggg acggtgccca ccacgttgag cgggttctcg
     4921 tagtggatcg tgccgcagga cgagcagatc gcccgctcgc ggttgtcctc gggcggctcg
     4981 cggtaggcgg tcggcgcgcc gcaggcgcgg caatggcgga tcgagcgggg gctgaacatg
     5041 ggcggtctcg cgggttcggg ccagtgtagc gggcatcccc acatcccagt gcggcgggcc
     5101 ctgcgtggca tagtcgtcgg ctcagtcccg gaaagcccgt catgaccgat gtcatcaccg
     5161 ttcccgtcgt cccgcgcgtg cccgtgcgcg ggggcggcca gttccccgtc caccgcatct
     5221 actgcgtcgg ccgcaactac gcggagcacg ccgccgagat gggccacgac gcgcgcgaga
     5281 gcccgttctt cttcatgaag ccggccgacg tcgtgctgcc cgtgcgcgcc ggcgagaccg
     5341 gcttcatgga ctacccgagc ctcaccagcg acctgcacca cgaggtcgag ctggtggtgg
     5401 ccatcggcga cgccgccgcc ggcaacggcc gcgacgtggc cgaggaggac gccccgcgcc
     5461 tgatcttcgg ctacgccgtc ggcctggaca tgacccggcg cgacctgcag gccgtggcca
     5521 agaaggccgg ccggccgtgg tcgatcgcca agggcttcga ccagtcggcg ccgatcggcg
     5581 agatcgtccg catcgaggag accggcgagc tgggcgccgg cgccatcacg ctggacgtcg
     5641 acggcgcacc gcgccagaag ggcgacctgt ccgacctgat ctggaacgtg cgcgagacca
     5701 tttcctggct cagccgggcg tggacgctca agccgggcga cctgatcttc accggcacgc
     5761 ccgcgggcgt cgccgccgtc ggccgcggcc agacgctcac ggggcgcatc gccggcctgg
     5821 gcgagctcgt cgtcacgctg cgctgaagga gccgccatgg agctctacag ctacttccgc
     5881 tcgtcggcca gctggcgcgt gcgcatcgcg ctggcgctca agggcctgga cgtggactac
     5941 cgcgcggtgc acctgctcag gaaggagcag tcggcgccgg actacgtcca gaagtcgacc
     6001 tccggcctcg tgccgctgct ggtgaccgac gacggcctgc ggctgcacca gtcgatggcc
     6061 atcatcgagt acctggacga gaccgtgccc gagccgccgc tgctcccgcg cgacgcggcc
     6121 ggccgggccc gcgtgcgcgc gctggcgcag gacatcgcct gcgagatcca tccgctgaac
     6181 aacacgcgcg tgctgggcta cctcacgaac acgctgggag tcggcgacga ggcgaagtcg
     6241 gcgtggatcc ggcgctgggt cgagggcggc ctcgaggtcg tcgagcgcca gctcgcggcg
     6301 cggccggcca cgttctgcga cggcgaggcg ccggggctgg ccgactgcgt gctcgtgccg
     6361 cagatcttca acgcccagcg cttcaagtgc gagctgggcc atgtgcccaa cgtcatgcgc
     6421 gtgcacgcgg cctgcatggc gctgccggcg ttcgccggca cgcagccctc ggcctgcccc
     6481 gacgcggaat agcacgccat gccgctcgag atcgtcgacc cgctgccgct ggacgccttc
     6541 ttccgcccgg tgagctggcc ccgcggcacc ggcgcgctga tgaccacgcg caagggcggt
     6601 gtcagcgcgg ccccgttcga ttcgctgaac ctgaaggcgc agagcgccga tccgctggac
     6661 cagatcgccg tcgccgagaa ccgccgccgc ttcgcggcca cgctgggcgc cgcgccggtc
     6721 ttcctcgacc aggtgcacgg cacgcgcgtc gtgcgcctcg cgacgagcca tctcgggccc
     6781 gaggccccca tcgagcaggc cgacgccagc atcaccacgg tgccgggcat cgcctgcacc
     6841 gtgatggtgg ccgactgcct gcccgtgctg ttcgccacgc gcgacggccg ggcggtgggc
     6901 gccgcgcacg cgggctggcg cgggctggcc gcgggcgtgc tggaggccac cgtcgatgcg
     6961 atccgcgccg cggtgccggt accggcgagc gacatcgtcg cgtggctggg ggcgtccatc
     7021 gggccgacgg cgttcgaggt cggcgccgac gtgccggccg ccttccacga gggcgaggcg
     7081 cgccacttcg tgccggcgcc cgcgcgcgac ggcgtcccga aatggcacgc cgacctgcct
     7141 gcgctggccg aggggcgtct gcgccggctc gggttgaccg aggtcagcgg ctgcggcgaa
     7201 tgcacggtca gcgacgcgtc acgcttcttc tcgtaccggc gcgacggcgt caccggccgc
     7261 ctggccgccg cggcctggat cgacgtcgcc tgaagcgcca gcggcacggc gcgccttcgc
     7321 gcgtgccctg gcgttcccga tgtacagcac gatgcccagg ggcagcgcgc catagaggaa
     7381 cagggtgacg atcgcgcccg gcaccgagcc gcccggtccg agggcctcgg ccagcaccat
     7441 catgaggacc acgtagatcc agccggcggc gacgatcagc acggtcgtgc ttcttgcggg
     7501 agggtcatcg ttcgtggtcc ggtaagcaat cttgcgcaat ggtagtttgt cacgtccctg
     7561 gggtaagctc gggtggctac aacgcgatct gatgatctga aagtccacgc ctgaaccgaa
     7621 ccggccgctg cccgaccgcg gcccgattcc ggggcctgaa ggcgtggcag aggagagtga
     7681 cgatggcccg cagtgccccc accaagacag ccgcccgcac ggctgcccgc cccgcacgcc
     7741 gcgcaccgcg cgcccgagcg gcggcggcgc cgtccgacaa ggccgtcgaa gccggcatcg
     7801 cgcgcgccac cgagcgcgcc ggcgccgcgc cgcgcgcgac cacgcaggcc cacgggcccg
     7861 aggccttcgg cgccgcggtc ggcggcttcc tcagttcgct cagcggcctg caggtgcccc
     7921 aggccacgct gtcgcaactg caggagcagt atgtgaacca cgcgacgtcg ctgtggaacg
     7981 gcgccgtcga ccgcatgccg ctgttcggca cgcaggacaa gccggccgag gccgcggtca
     8041 tcggcgaccg ccgtttctcc gccgaggact ggacgaagaa cccggccgcc gcgttcaccg
     8101 cgcagatgta cctgctcaac gcgcgcacgc tgacgcagct ggccgacagc ctgcagggcg
     8161 acgagaagac caaggcgcgc gtgcgcttcg ccgtgcagca gttcgtcgat gccgccagcc
     8221 ccgccaacta cctcgcgctg aaccccgagg cgcagcgcaa ggcgatcgag acgcagggcg
     8281 agagcctcgc ccagggcctg cagcagctgt ggggcgacgt gcagcagggc cacctgtcgc
     8341 agaccgacga atcggtgttc gaggtggggc gcaacgtcgc caccaccgaa ggctcggtcg
     8401 tgttcgagaa cgactacctg cagctgatcg aatacaagcc gctcacgccc agggtgcacg
     8461 cgcggccgat gctgttcgtg cccccgtgca tcaacaagta ctacatcctc gacctgcagc
     8521 cgtccaactc gctgatccgc cacacggtcg ccgaaggcca tcgcaccttc gtcatcagct
     8581 ggcgcaatcc cgacgagtcc atgcaggacc gcacctggga cgactacatc gagaacgcgg
     8641 tgatccgctc gatcgaggtg gtgcgcgaga tcaccggcgc caggacgatc aacacgctgg
     8701 gcttctgcgt cggcggcacg ctgctggcca ccggcctggg cgtgctggcc gcgcgcggcc
     8761 agcatccggc cgagagcctg acgctgctca cgacattcct cgatttctcc aacaccggcg
     8821 tgctggacat cttcgtggac gaggcctcgg tgcaactgcg cgagatgacc ctcggcgcgc
     8881 aggcgcccgg cggccccaag ctgctcaagg gcggcgagct ggccagcacc ttcagcttcc
     8941 tgcgccccaa cgacctcgtg tggaactacg tcgtcggcaa ctacctcaag ggcgacaagc
     9001 cgccggcatt cgacctgctg tactggaaca gcgacgcgac caacatgccg ggcccgatgt
     9061 actgctggta cctgcgcaac acctacctgg agaacgcgct gcgtgaaccg ggcgcgctga
     9121 cggtgtgcgg cgagaagctt gacctgtcgc aaatcaaggc gccggcattc atctatggtt
     9181 cgcgggagga ccacatcgtg ccgtggaagg gcgcctggga gtccacgaag atcctgggcg
     9241 gaaagacgcg cttcgtcctc ggtgcctccg gccacatcgc cggcgtcatc aatccgccca
     9301 aggccggcaa gcgcagccac tggaccaacg acaagcccgc cgtctcggcc gacgcctggc
     9361 ttgccggcgc caccgagcac cccggcagct ggtggacggg ctggtcgcag tggctggcgg
     9421 gctttggcgg cggcgatgcg cccgcgccga agggcccggg cggccacggc tacaaggcca
     9481 tcgagccggc ccctggccgc tacgtgcaac aaaaagcctg acaaccccct cgatcaggta
     9541 tttccggtca taccccatac cgtatcattc cgccccgcag gagaaccccc atgacccagg
     9601 acatcgtcat cgtcgccgcc gcccgcaccg ccgtcggcaa gttcggcggc tcgctcgcca
     9661 agatccccgc ttccgacctc ggcgccaccg tgctcaaggc gctgctggag cgcgccaggc
     9721 tcaccggcga gcaggtcagc gaggtcatcc tcggccaggt gctgcaggcc ggcaccggcc
     9781 agaaccccgc gcgccaggcg gtcatcaagg ggggcctggg caacggcgtg ccggcgatga
     9841 cgatcaacaa ggtctgcggc tcgggcctga aggccgtgat gctggccgcg caggcgatcc
     9901 gcgacggcga cgccgacatc gtgctggccg gcggccagga gagcatgagc ctcgcgccgc
     9961 acgtgctgcc gaactcgcgc gacggccagc gcatgggcga ctggaagctg gtcgacacca
    10021 tgatcaacga cggcctgtgg gacgtctaca accagtacca catgggcatc accgccgaga
    10081 acgtcgccaa gaagtacggc atctcgcgcg agcagcagga cgcgctcgcc ttcgcttcgc
    10141 agcagaaggc cgcggcggcg caggacgccg gcaggttcaa ggacgagatc gtgccggtcg
    10201 cgatcccgca gaagaagggc gacccgatcc tgttcgacgc cgacgagttc atcaaccgca
    10261 agtccaccgc cgaggcgctg gcgggcctgc gtcccgcgtt cgacaaggcc ggctcggtca
    10321 cggccggcaa cgcctcgggc ctgaacgacg gcgcggccgg cgtggtggtg atgtccgccg
    10381 cgcgcgccga tgcgctgggc ctcaagccgc tggcgcgcat cgcgtcgtac gcgagcgccg
    10441 gcctggaccc cgcctacatg ggcatgggcc cggtgcccgc cgcgcgcaag gcgctcgagc
    10501 gcgccggctg gaagcctgcc gacctcgacc tgctcgagat caacgaggcc ttcgccgcgc
    10561 aggcctgcgc cgtgcaccag gagatgggct gggacacgag caaggtcaac gtcaacggcg
    10621 gcgcgatcgc catcgggcac ccgatcggtg cgtcgggcgc gcgcatcctc gtcacgctgc
    10681 tgcacgagat gcagcgccgc aacgcgagga agggcatcgc ctcgctgtgc atcggcggcg
    10741 gcatgggcgt cgcgctgacc gtcgagcgtc cctgatccgc gggcggccgc atcgggttcg
    10801 ccccgagagc ggccgactgt acaaagcgcg atagtcaatt cgccacccca acacaatcct
    10861 caaggagaca tgacatgagc cagaaagttg cgtacgtgac cggaggcatg ggcggcatcg
    10921 gcaccgccat ctgccagcgc ctgcatcgcg atggcttcaa ggtgatcgcc ggctgcggcc
    10981 cgagtcgtga ccacgccaag tggatcgccg aacagaaggc gctgggctac acgttccacg
    11041 cgtcggtcgg caatgtcggc gactgggatt cgacggtcgc cgcgttccag aaggtcaagg
    11101 ccgacctcgg cccgatcgac gtgctggtca acaacgccgg catcacgcgc gacggcatgt
    11161 ttcgcaagat gacccgcgcc gactgggatg cggtgatcga taccaacctg acgtcgctgt
    11221 tcaacgtgac caagcaggtg gtggcggaca tggtcgagaa gggctggggc cgcatcatcc
    11281 agatctcgtc ggtcaacggc gccaagggcc aggccggcca gaccaactac tcggcggcca
    11341 aggccggcat gcacggcttc tcgatggcgc tggccctgga gatggccaac aagggcgtca
    11401 ccgtcaacac ggtcagcccc ggctacatcg gcaccgacat ggtgatggcc atcaagcccg
    11461 aggtgctgga gaagatcgtc gccacgatcc cggtcaagcg cctgggcacg cccgacgaga
    11521 tcgcctccat cgtgggctgg ctcgcgggcc ccgagtcggg cttcacgacc ggcgcggaat
    11581 tcgcggtcaa cggcggcctg cacatgggct gatccggcgc gacccgttct cgccaggccc
    11641 cgatcgcgcg agcggtcggg gcctttctcg ttgcgcgcca cccataatgc gtccatgacc
    11701 aagacacttc gggaggagac gatggacgac atgccgcagc ccgccggacg cggcggcgcc
    11761 ccatcgcgcc gcagggaact cacgatgacg gccgcggcgt cccatcgccc cggcatcgat
    11821 cagccgcgcg gcatgttccg atgaaactgc cgctggtcat cgccatcgtg gtcgcgctcg
    11881 ggctggcgct cgccgcctgg ttcttccgcg ggtcgcccat gttcgcgggc gcggcgcgcg
    11941 cgatcggcgt ccggcacgag aacgcgacgc ccgacgccgc cgccacgttc aaggccgccg
    12001 gcgtgcacaa gtgcgtcggt gcccacggca cgagctacat cgacgggccc tgtccggcgg
    12061 gcatgcgcga ggccgcggcc agcggcggca cggtgaccgt cacgtcgttc ccgaagccgg
    12121 cgcccgcggc cgcctcgtcg gcgctgggcg gtcccatcgt caagccgatg gacccggatg
    12181 aa
//
LOCUS       DATCRO010000439         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_747554, whole genome shotgun sequence.
ACCESSION   DATCRO010000439 DATCRO010000000
VERSION     DATCRO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747554"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VIN75_11905"
     CDS             complement(<1..441)
                     /locus_tag="VIN75_11905"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY8974917.1"
                     /translation="MSETGLRARSLCHGTRRVLHARRGFTLIELLVAAAALAILASIA
                     VPAFFEQLARARRADVQAALLEDAGYMQHYYASHDAFGGTPPPQLPFPQAPRQGGVAY
                     VITLAVPSGDPSSFQLTATRAGAMARDPCGDLTLDHLGQRGLVPG"
     gene            complement(467..1108)
                     /locus_tag="VIN75_11910"
     CDS             complement(467..1108)
                     /locus_tag="VIN75_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974918.1"
                     /translation="MSAPPVFTPARAARVTRARARGFSLIVAMLMLAVIGLASAAIMR
                     NAVSGDQVANNNRLQTQASQYALLALRFCQAQLGLPPEARVARLLPVATPPAWTVQRN
                     WGGSGGDAAHTLSAAEIGSSVQPRVAPQCLAEATSLANTYTVTARGFSADFKADPSTG
                     ATRTGSAVWVQATVYADSETGATGQAPAAAGTVPAGPLLVRQRTWQQLLTPPF"
     gene            complement(1105..2025)
                     /locus_tag="VIN75_11915"
     CDS             complement(1105..2025)
                     /locus_tag="VIN75_11915"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY8974919.1"
                     /translation="MIGRRVPHRGGRLRGAHAGFTLIEMMVALAIGLAVIGALIAAYL
                     SSFASGRHSDAVVQITEDATLALNVMRQQVAQAGFSQAHGVGESGGLVLHGFPAVAGC
                     EAAGFVDLQAGIAAPGNCQAPSSDPATPDTIEVAYEGSVLPGKASNGILGGAGTPQPL
                     DCLGNSFPRTHDDATGDDYWLNDSKFYVADGSLYCHGPGNRAGAAIVQNVETLQVTYG
                     MAAAPAGAPGAGQIVYYDNAPPAGSPLWADVVSVKLCVQVRSAVKVLDKASSPTLGGW
                     IDCGNAQRTSTDGYLRRTFTTTIVLQNKLL"
     gene            complement(2022..2603)
                     /gene="pilV"
                     /locus_tag="VIN75_11920"
     CDS             complement(2022..2603)
                     /gene="pilV"
                     /locus_tag="VIN75_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY8974920.1"
                     /translation="MRRVPPRATRATRLRAAGFSLIEVLVAMFVVAMGILALAGLLQA
                     STRYSKMSELRSTATLLANDIADRIRANPIGGELGVGGYDLADHGFPSPLVPAHAPCA
                     SETPCSPGELAKADLADWTARVRATLPKGSTWVQFHAGHPPAPDSVDVWVGWADPLTL
                     APGISTDRSGTECPASWKAAEPSVRCVYLQVAL"
     gene            complement(2606..>3191)
                     /locus_tag="VIN75_11925"
     CDS             complement(2606..>3191)
                     /locus_tag="VIN75_11925"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY8974921.1"
                     /translation="ALMNPTPVAAAESAARRSCRRPAGFTLVELMVTVAVAGLVVGLG
                     VPSFLRTLARHAIASQAEELQDAVRVGRNEAMKRSGPVVLCRTEESNPTHCAGSGGSW
                     QTWLLFTDAARSGTFAAGDSVLRQKQDVSRRTTVTADAASIRFEATGIAHADTGSAVF
                     VLAERGASDRAQRRQVCVNPRGEVAIVGGDARCP"
BASE COUNT          431 a         1119 c         1236 g          405 t
ORIGIN      
        1 gccgggcacg agcccgcgct ggccgaggtg gtcgagcgtg aggtcgccgc aggggtcgcg
       61 cgccatggcg ccggcgcgcg tggcggtcag ctggaacgac gacggatcgc cggagggcac
      121 ggccagcgtg atgacgtagg cgacgccgcc ctggcgtggg gcctgcggga acggcagctg
      181 cggcggcggg gtgccgccga acgcgtcgtg cgaggcgtag tagtgctgca tgtagccggc
      241 gtcctcgagc agcgcggcct gcacgtcggc ccggcgggcg cgcgcgagct gttcgaagaa
      301 ggccggcacc gcgatcgagg ccaggatggc cagcgccgcg gcggcgacga gcagctcgat
      361 caacgtgaag ccacgccgcg cgtgaaggac acggcgcgtg ccatggcaaa gcgagcgcgc
      421 gcgcaagccg gtctccgaca tgtggatgaa ttgacgtcgg gccggatcag aacggcggcg
      481 tcagcagctg ctgccacgtg cgctggcgca ccagcagcgg cccggccggc acggtgccgg
      541 cggccgcggg cgcctggcca gtggcgccag tctcgctgtc ggcgtagacc gtcgcctgca
      601 cccacacggc cgaaccggtg cgcgtggccc cggtcgacgg atcggccttg aagtcggcgc
      661 tgaagccgcg cgccgtgacg gtgtaggtgt tggccaggct cgtcgcctcc gccaggcact
      721 gcggtgcgac gcgcggctgc acgctggaac cgatctcggc ggccgacagc gtgtgcgcgg
      781 cgtcgccgcc ggacccgccc cagttgcgct gcaccgtcca ggccggcggc gtggccacgg
      841 gcagcagtcg cgcgacgcgc gcttcgggcg ggagcccgag ctgggcctgg cagaagcgca
      901 gcgcgagcag ggcgtactgg ctcgcctggg tctgcagccg gttgttgttg gccacctggt
      961 cgccgctgac ggcattgcgc atgatggccg ccgacgcgag gccgatcacc gcgagcatca
     1021 gcatcgccac gatgagcgag aaccctcgcg cgcgagcacg cgtgacgcgc gccgcgcggg
     1081 cgggggtgaa gacgggtggc gcgctcacag cagcttgttc tgcaacacga tggtggtggt
     1141 gaacgtgcgg cgcaggtagc cgtcggtgga cgtgcgctgc gcgttgccgc agtcgatcca
     1201 gccgcccagc gtcggcgacg aggccttgtc cagcaccttg accgcgctgc gcacctgcac
     1261 gcacagcttg accgacacga cgtcggccca cagcggcgag ccggccggcg gcgcgttgtc
     1321 gtagtagacg atctggcccg cgcctggcgc gcccgccggc gccgcggcca tgccgtaggt
     1381 gacctgcagc gtctcgacgt tctgcacgat ggcggcgccg gcccggttgc ccgggccgtg
     1441 gcagtacagg ctgccgtcgg cgacgtagaa cttgctgtcg ttgagccagt agtcgtcgcc
     1501 agtggcgtcg tcgtgcgtcc tgggaaaact gttgcccagg cagtcgagcg gctgcggcgt
     1561 gcccgcgccg cccaggatgc cgttggacgc cttgccgggc agcacgctgc cttcgtaggc
     1621 gacctcgatc gtgtcgggcg tggccgggtc ggacgacggc gcctggcagt tgccgggcgc
     1681 ggcgatgccc gcctgcaggt cgacgaagcc ggccgcctcg caacccgcga cggcggggaa
     1741 cccgtgcagc accagcccgc cgctctcgcc cacgccgtgc gcctggctga agccggcctg
     1801 cgcgacctgc tggcgcatga cgttgagcgc gagcgtggcg tcctcggtga tctgcaccac
     1861 ggcgtcggaa tggcggccgc tggcgaacga gctgaggtag gccgcgatca gcgcgccgat
     1921 gaccgccagg ccgatggcca gcgccaccat catctcgatc agagtgaaac cggcgtgcgc
     1981 gccgcgcagg cggccgccgc gatgcgggac gcgtcgaccg atcacagcgc cacctgcagg
     2041 tagacgcagc gcacggacgg ctcggccgcc ttccaggagg cggggcactc ggtgcccgag
     2101 cgatcggtgg agatgcccgg cgccagcgtg agcggatcgg cccagcccac ccacacgtcg
     2161 acggagtcgg gtgcgggcgg atggccggcg tggaactgca cccacgtcga gcccttgggc
     2221 agcgtcgccc ggacgcgggc cgtccagtcg gccaggtcgg ccttggccag ctcgcccggg
     2281 ctgcagggcg tctcgctggc gcagggcgcg tgcgccggca ccagcgggct ggggaagccg
     2341 tggtcggcca ggtcgtagcc gccgacgccc agttcgccgc cgatcgggtt ggcgcggatg
     2401 cggtcagcga tgtcgttggc cagcagcgtg gccgtcgagc gcagctcgct catcttcgag
     2461 tagcgcgtgg acgcctgcag cagcccggcc agcgcgagga tgcccatcgc gacgacgaac
     2521 atcgccacga gcacctcgat cagcgagaag cccgccgcgc gcaggcgcgt cgcgcgggtc
     2581 gcacgcgggg ggacacgccg catcgtcacg ggcaccgggc gtcgcctccg acgatcgcga
     2641 cctcgccgcg cgggttgacg cacacctggc gccgttgtgc gcggtcgctc gcgccgcgct
     2701 cggccagcac gaagaccgcg ctgcccgtgt ccgcatgcgc gatgccggtg gcctcgaagc
     2761 ggatgctcgc ggcgtcggcc gtgacggtgg tgcgccggga cacgtcctgc ttctggcgca
     2821 gcaccgaatc gcccgccgcg aacgtgccgc tgcgggcggc gtcggtgaac aggagccagg
     2881 tctgccagct gccgcccgag ccggcgcagt gcgtcgggtt gctttcctcg gtgcggcaca
     2941 gcaccaccgg gccgctgcgc ttcatcgcct cgttgcggcc gacgcgcacg gcgtcctgca
     3001 gttcctcggc ctgcgaggcg atggcgtggc gcgccagcgt gcgcaggaac gacggcacgc
     3061 ccagcccgac gaccaggccg gcgacggcca cggtgaccat cagctcgacc agcgtgaagc
     3121 cggccgggcg acggcaggag cgtcgggcgg cggactcggc ggcggcaacg ggagtgggat
     3181 tcatgagggc g
//
LOCUS       DATCRO010000440         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_994813, whole genome shotgun sequence.
ACCESSION   DATCRO010000440 DATCRO010000000
VERSION     DATCRO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994813"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..337
                     /locus_tag="VIN75_11930"
     CDS             95..337
                     /locus_tag="VIN75_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974922.1"
                     /translation="MTTATAATKIAPSLQEALLAAVDALGRRRACDIPEHTIDRLVLL
                     GWMRWSAGSLRLTEAGEAMVVKLQAALLDEDERLAA"
     gene            complement(525..887)
                     /locus_tag="VIN75_11935"
     CDS             complement(525..887)
                     /locus_tag="VIN75_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974923.1"
                     /translation="MPYVRRDLMGRIESVHRTSAADAVEYLDAADPELKRFLREEFGA
                     PEPSRAGTELLPVFEEVLQLLLAKGLVTVEELSENARATWTHVLEARQPPSATRFSAS
                     GFVEIIDDSAFGGLPDQR"
     gene            1109..2299
                     /locus_tag="VIN75_11940"
     CDS             1109..2299
                     /locus_tag="VIN75_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8974924.1"
                     /translation="MQVLEGIKVVELGQLIAGPFAAKTLADFGARVIKVEPPGTGDAL
                     RKWRKLREGTSVWWDAQSRNKESVCIDLRVPEGQALVRELVRDADVLIENFRPGALEG
                     WGLGWDALHALNPKLVMLSLSGFGQTGPRAAEPGFAAVAEAFSGLRYLNAEPGRTPVR
                     SGVSLGDTVAGVHGALGVMLALYARDARGGQGQRIDLALYEAMFNLSESLLPEYDVFG
                     EIRRPAGGALPGITPSNAYACADGQAVLIAGNGDSIFKRLMLMIGRKDLADDPALAAN
                     DGRSARAAELDAAIGAWTAQLPLDDVLARMRDAGVPASRVYTIAEIAADEHYAARGNI
                     ERIPAMNGGTIAAPGIVPKLSDTPGAVRHAAPRLGQHTDEVLAHAGLSAERIAQLRRD
                     GVIE"
     gene            2296..2445
                     /locus_tag="VIN75_11945"
     CDS             2296..2445
                     /locus_tag="VIN75_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974925.1"
                     /translation="MSRILVNEVAPRGGLQMEQRFVPTHEKIAFVDALSDCGLARIEV
                     TARCW"
     gene            complement(2504..>3081)
                     /locus_tag="VIN75_11950"
     CDS             complement(2504..>3081)
                     /locus_tag="VIN75_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974926.1"
                     /translation="FAQCKDSATSSINGAISITLAGTPTATQFTASANFQNVAVVQDG
                     VTSTIAGTVTVSENDTALDTLTTLSVGSGGLTAATASSGYNDSVSFASGFVIQTDEAT
                     SGASTTVSLSGTMTAQSLGGSITISTPQALVQLSSDTYPSAGQVIVTGASGSAVRATV
                     LDNTQVKVDLDANGDGTYESTATIAWTSLIV"
BASE COUNT          447 a         1108 c         1086 g          440 t
ORIGIN      
        1 ccgagtcggc gctgtcctac aagacgacgc tgccgactcc gactgtgcct ccgcggtcgc
       61 atcgctatcg ttccgtcacc aacaaggaac gcccatgacg accgccaccg ccgccaccaa
      121 gatcgccccg tcgctgcagg aagccctgct cgccgccgtc gacgccctcg gccgccgccg
      181 cgcctgcgac atccccgaac acacgatcga ccgcctcgtg ctgctgggct ggatgcgctg
      241 gagcgcgggc tcgttgcgcc tgaccgaagc cggtgaagcc atggtcgtga agctgcaggc
      301 cgcgcttctc gacgaagacg agcgcctggc cgcctgaccg gccggtgacg cgcccaggcc
      361 cggcctggcg cgcgcggccc gccgccgccc gaacgcggga gaatgggccg cccatcaacc
      421 tccaacgcct cgatgggcat catccggctt cgacgcgatg ggcctcacgg cggccaacgg
      481 catgggcttg gcgatgctgc ccgaacgagg cctgggcggc gcacctagcg ttggtccggc
      541 aacccgccga aggcgctgtc gtcgatgatc tccacgaagc ccgacgccga gaagcgggtc
      601 gcgctcggcg gctgcctggc ctccaggacg tgcgtccagg tcgcccgggc gttctccgac
      661 agctcctcca ccgtcaccaa gcccttggcc agcagcagct ggagcacctc ctcgaacacg
      721 gggagcagct cggtccccgc ccgggacggc tcgggcgcgc cgaactcctc ccgcaggaag
      781 cgcttgagct cggggtcggc ggcatcgagg tattcgacgg cgtcggcggc cgacgtgcgg
      841 tgcacgctct cgatgcggcc catgagatcg cggcggacat atggcatgga ggtctccctg
      901 accggatttt ctggcgctag tctggcactc gacagtgcgt ctggccactg gcaggtctta
      961 catggcgcac cccggcggaa tctggcagcc cgcggaacag cccaccgccg ctttcacgcc
     1021 gcgcgaaagc tcgcttggca agtcctgcct tccgccggcc gcggcgcgtg accacaatcg
     1081 gtcccaccgt cacgaaagac agccggccat gcaggtgctc gaaggaatca aggtcgtcga
     1141 actgggccag ctcatcgcgg ggccgttcgc cgcgaagact ctggccgact tcggcgcgcg
     1201 cgtcatcaag gtggaaccgc ccgggacggg cgacgcgttg cgcaagtggc gcaagctgcg
     1261 cgagggcacg tccgtgtggt gggacgccca gtcgcgcaac aaggagtcgg tgtgcatcga
     1321 cctgcgcgtg cccgagggcc aggcgctggt gcgcgagctg gtgcgcgatg ccgacgtgct
     1381 gatcgagaac ttccgcccgg gcgcgctcga gggctggggc ctgggctggg acgcgctgca
     1441 cgcgctcaac ccgaagctcg tcatgctgag cctgtcgggc ttcggccaga cgggcccgcg
     1501 cgccgccgag ccgggcttcg cggcagtggc cgaagcgttc agcggcctgc gctacctcaa
     1561 cgccgagccg ggccgcacgc cggtgcgcag cggcgtgtcg ctgggtgaca ccgtggccgg
     1621 cgtgcacggc gcgctgggcg tgatgctggc cctgtacgcg cgtgatgcgc gcggcgggca
     1681 gggccagcgc atcgacctcg cgctgtacga ggcgatgttc aacctttcgg aaagcctgct
     1741 gcccgagtac gacgtgttcg gcgagatccg tcgccccgcc ggcggcgcgc tgccgggcat
     1801 cacgccgtcc aacgcctacg cctgcgccga cggccaggcc gtgctgatcg ccggcaacgg
     1861 cgacagcatc ttcaagcggc tgatgctgat gatcggccgc aaggacctcg ccgacgatcc
     1921 ggcgctggcc gccaacgacg gccgctccgc gcgagccgcc gagctcgacg ccgccatcgg
     1981 cgcgtggacg gcgcaactgc cgctggacga cgtgctcgca cggatgcgcg acgcgggcgt
     2041 gcccgcgagc cgcgtctaca ccatcgccga gatcgccgcc gacgagcact acgccgcgcg
     2101 cggcaacatc gagcgcatcc ccgcgatgaa cggtggcacc atcgccgcgc cgggcatcgt
     2161 gcccaagctg tcggacacgc ccggcgccgt gcgacatgcg gccccgcggc tgggccagca
     2221 caccgacgag gtgctcgcgc acgccggcct gtcggccgag cgcatcgcgc agctgcgccg
     2281 ggatggagtc atcgaatgag ccgcatcctc gtcaacgagg tcgcgccgcg cggcggcctg
     2341 cagatggagc agcgcttcgt gcccacgcac gagaagatcg ccttcgtcga cgcgctgtcg
     2401 gactgcggct tggccaggat cgaggtgacg gcgcgatgct ggtgatgaag gcgctgcccg
     2461 cggccgcgat gggcgcggcg cgcggcccgg cgccgcgtcg atctcagacg atcagcgacg
     2521 tccaggcgat ggtggccgtg ctctcgtacg tgccgtcgcc gttggcgtcc aggtccacct
     2581 tgacctgggt gttgtccagc accgtggccc gcaccgcgct gcccgaggcg ccggtcacga
     2641 tgacctggcc ggcgcttgga taggtgtccg aggacagctg caccagcgcc tgcggcgtcg
     2701 agatcgtgat gctgccgccc agcgactgcg cggtcatcgt gccggacagc gacaccgtgg
     2761 tgctcgcacc cgaggtcgcc tcgtcggtct ggatgacgaa gcccgatgcg aacgagaccg
     2821 agtcgttgta gccggacgag gccgtcgccg cggtcaggcc gccgctgccc acgctcagcg
     2881 tggtcagcgt gtcgagtgcc gtgtcgttct ccgagaccgt gacagtgccg gcgatcgtcg
     2941 atgtcacgcc gtcctgcacc accgcgacgt tctggaagtt cgcgctggcg gtgaactgcg
     3001 tggcggtcgg cgtgccggcc agcgtgatcg agatcgcgcc gttgatcgag gaggtcgcgc
     3061 tgtccttgca ctgcgcgaag c
//
LOCUS       DATCRO010000441         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_859903, whole genome shotgun sequence.
ACCESSION   DATCRO010000441 DATCRO010000000
VERSION     DATCRO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859903"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /gene="hscB"
                     /locus_tag="VIN75_11955"
     CDS             complement(<1..126)
                     /gene="hscB"
                     /locus_tag="VIN75_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515280.1"
                     /note="J-type co-chaperone that regulates the ATPase and
                     peptide-binding activity of Hsc66 chaperone; may function
                     in biogenesis of iron-sulfur proteins; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HEY8974927.1"
                     /translation="MSLQASDFELFGLPERFDVDLAALDAKWKQLQGAAHPDRFAT"
     gene            complement(123..608)
                     /locus_tag="VIN75_11960"
     CDS             complement(123..608)
                     /locus_tag="VIN75_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8974928.1"
                     /translation="MTLAIRPTPPEHPQVQALIAELDAYLSTIYPPEENYLLDLSGLQ
                     QPGVTFLGAWMGETLVGCGAVRAMPGEPATDGRPYGEVKRMFVSPAQRGARIGARILE
                     QLEAALRAQGIDRALMETGEELVQAARMYRAAGYVERREFGEYPDNGSSLFLEKRWTT
                     A"
     gene            complement(683..1006)
                     /gene="iscA"
                     /locus_tag="VIN75_11965"
     CDS             complement(683..1006)
                     /gene="iscA"
                     /locus_tag="VIN75_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY8974929.1"
                     /translation="MAVTLTERAARHVTRYITKRGRGVGVRLGVKTTGCSGLAYKLEY
                     ADDVAPEDLVFEGHGVKVLVDPKSLPYIDGTELDFVREGLNEGFKFHNPREKDKCGCG
                     ESFRV"
     gene            complement(1018..1440)
                     /gene="iscU"
                     /locus_tag="VIN75_11970"
     CDS             complement(1018..1440)
                     /gene="iscU"
                     /locus_tag="VIN75_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY8974930.1"
                     /translation="MAYSDKVIDHYENPRNVGAFDKGDDTVGTGMVGAPACGDVMKLQ
                     IKVNPATGLIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAMAIRNTQIAEELALPP
                     VKIHCSILAEDAIKAAVSDYKARHGAEAPAGEAAPLNA"
     gene            complement(1479..2693)
                     /locus_tag="VIN75_11975"
     CDS             complement(1479..2693)
                     /locus_tag="VIN75_11975"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY8974931.1"
                     /translation="MTPHFPIYMDYGATTPVDPRVVDAMIPWLREHFGNPSSRSHAWG
                     WEAEEAVERARGQVAALIGADPREIVWTSGATESNNLAIKGAANFYSTRGKHLITVKT
                     EHKAVLDVMRELERQGFECTYLDVEPNGLLDIEKFKAAIRPDTILASVMFVNNEIGVI
                     QDIPAIGAICRERGIIFHVDAAQATGKVDIDVAKLPVDLMSLASHKTYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGMRSGTLPTHQCVGMGEAFRIARDEMGAERERIRALQQR
                     LYTGLKDIEQVFVNGDLERRVPHNLNLSFNYVEGESLIMGVKGLAVSSGSACTSASLE
                     PSYVLRALGRSDELAHSSLRMTIGRFTTEEEIDSAVAMLKDTVARLRELSPLWDMYRD
                     GIDISAIQWAAH"
     gene            complement(2710..3243)
                     /gene="iscR"
                     /locus_tag="VIN75_11980"
     CDS             complement(2710..3243)
                     /gene="iscR"
                     /locus_tag="VIN75_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEY8974932.1"
                     /translation="MRLTTKGRFAVTAMIDLALRSSNGPVALAAISQRQQISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYSLGRGAESITVADIIVAVDEPIDATGCAGKENCMGDD
                     TGRCMTHDLWASLNVKMIEFLDSISLKKLVDEQLAKGVSIEEVPVRRAISSQPVVKPI
                     KVNAPNSVFALGNAFSK"
     gene            complement(3500..3979)
                     /locus_tag="VIN75_11985"
     CDS             complement(3500..3979)
                     /locus_tag="VIN75_11985"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY8974933.1"
                     /translation="MKPAPVRVLMVCLGNICRSPTAEAMLRKKAHEAGLDGRIEVDSA
                     GTSDYHVDSPPDRRAVAHGERRGLRMQQLRGRQVERGDFDRFDWILAMDEDNLAHLER
                     LRPAGSRAKVALLMSFAPDAGAREVPDPYCGGADGFEHVLELTAAAADGFIASVRSN"
     gene            complement(4059..4667)
                     /locus_tag="VIN75_11990"
     CDS             complement(4059..4667)
                     /locus_tag="VIN75_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974934.1"
                     /translation="MSLTKYVAAAVAALSFAGAAHADALYTSSAYGSIANTPDINWCS
                     SCGGQYQVLNPFSLSQSSVVTGLDIGATAWYGMNWNLTVQVWDSTHTNVLDTATFASG
                     SYTTNVLNANVNELTFTLPSWNLAAGNYYVSVVDPTDFAIASYNNTGTDFQYQIGIGQ
                     HDTSGAYVVSGVSAVPEGSELSLMLGGLALVGTAVRRRRLKA"
     gene            complement(4932..7094)
                     /gene="uvrB"
                     /locus_tag="VIN75_11995"
     CDS             complement(4932..7094)
                     /gene="uvrB"
                     /locus_tag="VIN75_11995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8974935.1"
                     /translation="MPIDSDVMPQEPTAAGYSHAAPVAAPGAEPPQGEFVSFPDSPFQ
                     LFQPYPPAGDQPQAIDKLVEGVNDGEVFQTLLGVTGSGKTFTMANVIASEARPALVLA
                     PNKTLAAQLYSEFREFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQM
                     RLSATKSVLERRDTIIVASVSAIYGIGKPEDYTQMRFILRNGDKLGQRDVIAQLIRMQ
                     YTRNETEFSRGTFRVRGDTIDVFPAEHSELAVRIELFDDEVESLQLLDPLTGQIRQKV
                     PRFTIYPSSHYVTPRERIVTAIDTIKEELRWRLDELVRNNKLVEAQRLEQRTRFDLEM
                     LQEVGHCKGIENYSRHLSGAAPGDPPPTLVDYMPKDAIMFIDESHVMMGQIGAMYKGD
                     RGRKENLVEYGFRLPSALDNRPLKFEEFERKMRQCVFVTATPADYEKNHAGQVVEQLV
                     RPTGLIDPIVQVRPASTQVDDVLQEIRERTSAGDRVLITTLTKKMAEQLTDYLTENGV
                     KVRYLHSDIDTVERVEILRDLRIGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFL
                     RAERSLIQTIGRAARNVNGQAILYADKMTDSMRKAIDETERRRARQIAHNTAMGIVPR
                     GIRKTIRDLIDGVAAERSGKDDLKFAQQAAEVEAMSEKDLGKRIKLLEKQMLEHAKNL
                     EFEKAARVRDQLALLREQAFGAAGHDTAVAAAAGTTRK"
     gene            7282..>7426
                     /locus_tag="VIN75_12000"
     CDS             7282..>7426
                     /locus_tag="VIN75_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009932860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid aminotransferase"
                     /protein_id="HEY8974936.1"
                     /translation="MSLFASVELAPRDPILGLNEQFNADTNPAKVNLGVGVYTDENGK
                     LPLL"
BASE COUNT         1105 a         2430 c         2589 g         1302 t
ORIGIN      
        1 ggtggcgaag cgatcggggt gcgccgcgcc ctgcagctgc ttccacttcg cgtcgagcgc
       61 ggccaggtcg acgtcgaagc gttccggcag gccgaacagc tcgaagtcgc tcgcctgtag
      121 gctcaggccg tggtccatcg cttctccagg aacagcgacg acccgttgtc cgggtactcg
      181 ccgaactccc tgcgctcgac gtagccggcg gcgcggtaca tgcgcgcggc ctggacgagc
      241 tcctcgcccg tctccatcag cgcacggtcg atgccctgtg ccctcagggc ggcctcgagc
      301 tgctccagga tgcgggcgcc gatgcgcgcg ccgcgctgcg ccggcgacac gaacatgcgc
      361 ttgacctcgc cgtagggccg gccgtcggtg gcgggttcgc cgggcatcgc gcgcacggcg
      421 ccgcagccca ccagcgtctc gcccatccag gcgccgagga aggtgacgcc gggctgctgc
      481 aggccggaca ggtccagcag gtagttctct tccggcgggt agatcgtcga caggtaggcg
      541 tcgagctcgg cgatcagcgc ctgcacctgc ggatgctcgg gcggcgtggg acggatggcc
      601 agggtcatgg gaggaaacgg ttctcgccaa caaaaaggcg cgggcagctg ccgcgccttc
      661 gtggcgtcca ggggaaacaa ggtcagacgc ggaacgactc gccgcagccg cacttgtcct
      721 tttcgcgcgg gttgtggaac ttgaagccct cgttgagccc ttcgcgcacg aagtcgagct
      781 ccgtgccgtc gatgtagggc aggctcttcg ggtcgaccag caccttgacg ccgtggcctt
      841 cgaagacgag gtcttccggc gccacgtcgt cggcgtactc gagcttgtag gccaggccgg
      901 agcagcccgt cgtcttcacg ccgagccgca cgccgacgcc gcggccgcgc ttggtgatgt
      961 agcgcgtcac gtgcctggca gcgcgttcgg tcagggtcac cgccatcgtc gcgggcctca
     1021 ggcgttcagc ggcgcagctt cgccggccgg cgcctcggcg ccgtggcggg ccttgtagtc
     1081 gctgaccgcg gccttgatcg cgtcctcggc caggatcgag cagtggatct tgacgggggg
     1141 cagcgcgagc tcctcggcga tctgcgtgtt gcggatggcc atggcttcgt ccagcgactt
     1201 gcccttgacc cattcggtga ccagcgacga ggaggcgatg gccgagccgc agccgtaggt
     1261 cttgaagcgc gcgtcctcga tcaggcccgt ggccgggttg accttgatct gcagcttcat
     1321 cacatcgccg cacgccgggg cgccgaccat gccggtgccg acggtgtcgt cacccttgtc
     1381 gaacgcgccc acgttgcgcg ggttctcgta gtggtcgatg accttgtcgc tgtatgccat
     1441 gttcttctac cctcaacgaa tggttatgtc ggaagcgatc agtgggcggc ccactggatc
     1501 gcgctgatgt cgatgccgtc gcggtacatg tcccacagcg gggacagctc gcgcaggcgc
     1561 gcgacggtgt ccttgagcat cgccaccgcg gagtcgatct cttcctcggt ggtgaagcgg
     1621 ccgatggtca tgcgcaggga cgagtgcgcc agctcgtcgc tgcggcccag ggcgcgcagc
     1681 acgtagctgg gctcgaggct ggcggacgtg caggccgagc ccgacgacac cgccaggccc
     1741 ttgacgccca tgatgagcga ctcgccctcg acgtagttga aggacaggtt caggttgtgc
     1801 ggcacgcggc gttccaggtc gccgttgacg aacacctgct cgatgtcctt caggcccgtg
     1861 tacaggcgct gttgcagcgc gcggatgcgc tcacgctcgg cgcccatctc gtccctggcg
     1921 atgcggaacg cctcgcccat gcccacgcac tggtgcgtgg gcagcgtgcc cgagcgcatg
     1981 ccgcgctcgt ggccgccgcc gtgcatctgc gcctccaggc gcacgcgcgg cttgcggcgc
     2041 acgtacagcg cgccgatgcc cttggggccg taggtcttgt gcgaggccag gctcatcagg
     2101 tcgaccggca gcttggcgac gtcgatgtcg accttgccgg tggcctgcgc cgcatccacg
     2161 tggaagatga tgccgcgctc gcggcagatc gcgccgatcg ccgggatgtc ctggatgacg
     2221 ccgatctcgt tgttcacgaa catgaccgag gcgaggatcg tgtccgggcg gatcgccgcc
     2281 ttgaacttct cgatgtccag caggccgttc ggctcgacgt cgaggtacgt gcactcgaag
     2341 ccctggcgct ccagctcgcg catcacgtcg agcacggcct tgtgctcggt cttgacggtg
     2401 atcaggtgct tgccgcgcgt gctgtagaag ttggccgcgc ccttgatggc gaggttgttc
     2461 gactcggtgg cgcccgacgt ccagacgatc tcccgcgggt cggccccgat cagcgcggcg
     2521 acctggccgc gcgcgcgctc cacggcctcc tcggcctccc agccccacgc gtgcgagcgc
     2581 gaggacgggt tgccgaagtg ctcgcgcagc caggggatca tcgcatcgac cacgcgcggg
     2641 tcgaccggcg tcgtcgcgcc gtagtccatg tagatcggga aatgcggggt catgagtcga
     2701 ggaacagaat tacttcgaga acgcgttgcc gagcgcgaac acggagttcg gcgcgttgac
     2761 cttgatcggc ttgacgaccg gctgcgagga gatcgcgcgg cgcaccggca cctcttcgat
     2821 ggacacgccc ttggcgagct gctcgtcgac cagcttcttg agcgagatcg aatcgaggaa
     2881 ctcgatcatc ttgacgttca ggctggccca caggtcgtgc gtcatgcaac ggccggtgtc
     2941 gtcgcccatg cagttctcct tgccggcgca acccgtggcg tcgatcggtt cgtcgaccgc
     3001 gacgatgatg tcggcgacgg tgatcgattc ggcgccgcgg ccgagcgagt agccgccgcc
     3061 ggggccgcgc gtgctctcga cgagctcgtg ccggcgcagc ttgccgaaca gctgttccag
     3121 gtacgacagc gagatctgct gacgctgcga gatggcagcg agggcgaccg gaccattgct
     3181 ggagcggagg gccagatcga tcattgccgt gacagcgaat cggcctttgg tggtcagacg
     3241 catggggtgc tctcctgggg gtgttggggg caatcggcta cggtccgcat cggggaggcg
     3301 atgagaggtc ggagagcagt gataaccctt ggttgattcc gagcgtttta gtcgattatg
     3361 ccagaagcct agcgattccg tcgactttgg gacgcagaaa tgttgcagcg cggcagagca
     3421 tgcgcctttt gcccctgacg cccggtggaa cagcccgaaa gcccattctg cgcaaagcgg
     3481 tcttcccgac cgcaaatgct caattcgaac gcacggaggc gatgaagccg tcggccgccg
     3541 cggcggtcag ctccagcacg tgttcgaacc cgtcggcgcc gccgcagtac gggtcgggca
     3601 cctcgcgggc gccggcgtcc ggcgcgaacg acatcagcag cgcgaccttg gcgcgcgagc
     3661 cggcgggccg caggcgctcc aggtgggcga ggttgtcctc atccatcgcc aggatccagt
     3721 cgaagcggtc gaagtcgccc cgctccacct ggcggccgcg cagctgctgc atccgcaggc
     3781 cgcggcgttc gccgtgggcg acggcacggc ggtcgggggg cgagtcgacg tggtagtcgg
     3841 acgtgccggc ggagtcgacc tcgatgcggc cgtccagccc ggcctcgtgc gccttcttgc
     3901 gcaacatcgc ttcagcggtg ggggaacggc agatgttccc caagcaaacc atcaggacgc
     3961 ggaccggtgc gggcttcatg cgtggattat cgggcgcatg aaaaaggccg cgccggcttg
     4021 caccggcgcg gcctggtcgc gcgaggacgc gcgcccggtc aggccttcag gcggcggcga
     4081 cgcacggccg tgccgaccag cgccaggccg cccagcatca gcgacagctc ggagccttcc
     4141 ggaacggcgg agacgccgct caccacgtag gcgcccgacg tgtcgtgctg accgataccg
     4201 atctggtact ggaagtcggt gccggtgttg ttgtagctgg cgatcgcgaa gtcggtcgga
     4261 tcgacgacgc tcacgtagta gttgccggcg gccaggttcc acgacggcag cgtgaaggtc
     4321 agctcattga cgttcgcgtt gaggacgttc gtcgtgtagc tgcccgaggc gaaggtcgcc
     4381 gtgtcgagca cgttcgtgtg ggtgctgtcc cagacctgga cggtcaggtt ccagttcatg
     4441 ccgtaccagg cggtcgcgcc gatgtccagg ccggtcacga cgctggactg cgacaggctg
     4501 aacgggttga gcacctggta ctggccgccg cacgacgagc accagttgat gtcgggcgtg
     4561 ttggcgatcg acccgtaggc cgaagaagtg tagagggcat cggcatgcgc cgcgccggca
     4621 aaggacaggg ccgcgacggc ggcggcaacg tacttggtca atgacatggt tgacttcatg
     4681 aagggttgtc ggcaccgcac tccctgggcg gtggtggcgc cggcgcgctc tgcagcgcga
     4741 cggcaggcgg attgtggagg gggcgtaaca gcatgtgtac gcgcgggaag agcgaacgac
     4801 ccaagccgtg acgaagttgg catagatgcg ctcaactcgc ctcaaatctg gccgaagtgc
     4861 accgcgaggc gatggctgcc tcgcggtcgc cccggccgcc gcgctcccgc cggagcggcg
     4921 ggccgggccg ctcacttgcg cgtcgtgccc gcggcggccg cgacggcggt gtcgtgcccc
     4981 gcggcgccga aggcctgctc gcgcagcagc gcgagctggt cgcgcacgcg cgccgccttc
     5041 tcgaattcga ggttcttcgc gtgctcgagc atctgctttt ccagcagctt gatgcgcttg
     5101 cccaggtcct tctcgctcat cgcctcgacc tcggcggcct gctgcgcgaa cttcaggtcg
     5161 tccttgcccg agcgctcggc cgcgacgccg tcgatcaggt cgcggatggt cttgcggatg
     5221 ccgcgcggca cgatgcccat ggcggtgttg tgagcgatct gcctggcgcg ccggcgctcg
     5281 gtctcgtcga tggccttgcg catcgagtcg gtcatcttgt cggcgtacag gatggcctgg
     5341 ccgttgacgt tgcgcgccgc gcggccgatg gtctggatca ggctgcgctc ggcgcgcagg
     5401 aagccttcct tgtcggcgtc gaggatggcc acgagggaga cctccgggat gtccaggccc
     5461 tcgcgcagca ggttgatgcc caccagcacg tcgaaggtgc cgatgcgcag gtcgcgcagg
     5521 atctccacgc gctcgaccgt gtcgatgtcc gagtgcaggt agcgcacctt gacgccgttc
     5581 tcggtgaggt agtcggtcag ctgctcggcc atcttcttgg tgagcgtggt gatcagcacg
     5641 cggtcgccgg cggacgtgcg ctcgcggatc tcctgcagca cgtcgtcgac ctgcgtgctg
     5701 gcgggacgca cctgcacgat cgggtcgatc aggcccgtgg gccgcaccag ctgctccacc
     5761 acctggccgg cgtggttctt ctcgtagtcg gcgggcgtgg cggtgacgaa cacgcactgg
     5821 cgcatcttgc gttcgaactc ctcgaacttc agcgggcggt tgtccagcgc cgacggcagc
     5881 cggaaaccat actcgaccag gttctccttg cgcccgcggt cgcctttgta catggcgccg
     5941 atctggccca tcatgacgtg gctctcgtcg atgaacatga tggcgtcctt gggcatgtag
     6001 tccaccagcg tgggcggcgg atcgcccggc gcggcgcccg acaggtggcg cgagtagttc
     6061 tcgatgccct tgcagtggcc gacctcctgc agcatctcca ggtcgaagcg cgtgcgctgt
     6121 tccaggcgct gcgcctccac gagcttgttg ttcctgacca gctcgtccag gcgccagcgc
     6181 agctcttcct tgatcgtgtc gatcgccgtg acgatgcgct cgcgcggcgt cacgtagtgg
     6241 ctggacggat agatcgtgaa gcgcgggacc ttctggcgga tctggccggt gagcggatcc
     6301 agcagctgca gcgactcgac ctcgtcgtcg aacagctcga tgcgcacggc gagctcggag
     6361 tgctccgccg ggaacacgtc gatcgtgtcg ccacgcacgc ggaacgtgcc gcgcgagaac
     6421 tcggtctcgt tgcgcgtgta ctgcatgcgg atcagctgcg cgatgacgtc gcgctggccg
     6481 agcttgtcgc cgttgcgcag gatgaagcgc atctgcgtgt agtcctcggg cttgccgatg
     6541 ccgtagatgg ccgacacgct cgccacgatg atggtgtcgc gccgctccag cacgctcttg
     6601 gtggccgaca gccgcatctg ctcgatgtgc tcgttgatcg acgagtcctt ctcgatgaac
     6661 aggtcgcgct gcgggacgta ggcctcgggc tggtagtagt cgtagtagct gacgaagtac
     6721 tcgaccgcgt tcttcgggaa gaactcccgg aactcgctgt acagctgcgc cgccagcgtc
     6781 ttgttgggcg ccagcacgag cgccggccgt gcctcgctgg cgatgacgtt ggccatggtg
     6841 aaggtcttgc cggagccggt cacgcccagc aaggtctgga acacctcgcc gtcgttgacg
     6901 ccttcgacga gcttgtcgat ggcctgcggc tggtcgcccg ccggcgggta cggctggaac
     6961 agctggaacg gcgaatcggg aaaggagacg aactcgccct ggggcggctc ggcgccgggc
     7021 gcggccacgg gcgcggcatg cgagtagccg gcggcggtag gctcctgcgg catcacgtca
     7081 gaatcaatcg gcatcgggtt atccctggct cttggcctaa aatcgagacg ctgccggtgg
     7141 tggtgcgtcc agtcgcgatc cggcgacggc caatccgaca gcgtagcgca acgtggcaga
     7201 caaagctcct gacacccgcg caacccttcc atctgccgcc atcttccggg cttttcaagc
     7261 ccattccagg aaccagactc catgtcgctg tttgcctccg tcgaactcgc cccgcgtgac
     7321 cccatcctcg gcctgaacga gcagttcaac gccgacacca acccggccaa ggtcaacctc
     7381 ggcgtgggcg tgtacaccga cgagaacggc aagctgccgc tgctgg
//
LOCUS       DATCRO010000442         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_27183, whole genome shotgun sequence.
ACCESSION   DATCRO010000442 DATCRO010000000
VERSION     DATCRO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27183"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1101)
                     /locus_tag="VIN75_12005"
     CDS             complement(166..1101)
                     /locus_tag="VIN75_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8974937.1"
                     /translation="MTTSLRPLSVLTLALAAAGAAHAAEPAVDIYGFVDVAVGKETGQ
                     GGTHMLDDAGSRLGFKGEQDVGHGLTASFQIEARFTPQTGEESKPFWKGGAYGALAGS
                     FGKVIAGRWWSQAFLKSQFAADPFGEATVGLNYGTVGCGGGSCVGAFWVDNSVSYENS
                     FGPVSVGVQMAEKPAPGDKNPVNGGVSYAQGGLYVGLGYEKSGVDDSSWSSATVNYDF
                     GAVKLYSGYGTGKDNAGASHRNAIVGFDVPVGDGHVIGAFDQHRVSSVTVTSKAALGY
                     QYSLNKNAKLFATVANDSKAASDKTGYDVGMIYSW"
     gene            1297..>2056
                     /locus_tag="VIN75_12010"
     CDS             1297..>2056
                     /locus_tag="VIN75_12010"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HEY8974938.1"
                     /translation="MTQSPEPLPAADPAQPTSLARSVALFGGAALLCVLGCICAVMTA
                     MLMAQAQERTLAWMDARVASVADSLDTHDQTARLLVERFAKVFADQFGRNFALDEAAG
                     KLSQLGIPLNERHGPCDKFKEFTGGEAAVLMAQGGRFMAISTSLVDARGERSLGWAIG
                     PGHPAYAALQKGQTWAGRGQLLGRSFEQRVQPVRDLQDRIVGALYIAFDLADFDSAIE
                     RRVNATRFFESGAAYVVDPGRNGEPARIVAPARLR"
BASE COUNT          310 a          698 c          726 g          322 t
ORIGIN      
        1 ggcgggcgga gccggatgcg cggcgcacgc gacgcgcctc gcgcgcggga cgcgagcggg
       61 ggcgcacggg tgcgcccggc gcgcgggggg gcgcgtgacg cgcgggcgcc atcgccggtg
      121 ggatgtcgcc ggaggcgccc cgtccccggg gcgcctcgtc ctggctcacc agctgtagat
      181 catcccgacg tcgtagccgg tcttgtccga ggccgccttg gaatcattgg ccaccgtggc
      241 gaacagcttg gcgttcttgt tgaggctgta ctggtagccc agcgccgcct tggacgtgac
      301 ggtcacgctc gagacgcggt gctggtcgaa ggcgccgatg acgtggccgt cgccgacggg
      361 cacgtcgaag ccgacgatcg cgttgcggtg gctggcgccc gcgttgtcct tgcccgtgcc
      421 gtagcccgag tacagcttga cggcgccgaa gtcgtagttg acggtggcgc tgctccacga
      481 cgagtcgtcc acgccggact tctcgtagcc caggccgacg tacagcccgc cctgcgcgta
      541 gctgacgccg ccgttgacgg ggttcttgtc gccgggggcc ggcttctcgg ccatctgcac
      601 gcccaccgac accgggccga agctgttctc gtaggagacg ctgttgtcga cccagaacgc
      661 gccgacgcag ctgccgccgc cgcaaccgac cgtgccgtag ttcaggccga cggtggcctc
      721 gccgaacggg tcggccgcga actgcgactt caggaaggcc tgcgaccacc agcggccggc
      781 gatgaccttg ccgaagctgc cggcgagcgc gccgtacgcg ccgcccttcc agaacggctt
      841 ggattcctcg ccggtctgcg gcgtgaagcg ggcctcgatc tggaacgagg cggtcaggcc
      901 gtggccgaca tcctgctcgc ccttgaagcc gaggcggctg ccggcgtcgt cgagcatgtg
      961 ggtgcccccc tggcccgtct ccttgccgac ggcgacgtcg acgaagccat agatgtcgac
     1021 cgcgggctcg gcggcgtggg cagcgcccgc cgcggcgagt gccagggtca ggacggaaag
     1081 gggtcgcaag gacgtcgtca tggtgtctcc agatgatgga attgatagat tgaattgata
     1141 agcctcatga tgtaaagcga tcatctggtg gcagatgacg gtctttttac tgcgatacag
     1201 ccgatgagac gtggagataa taaatcaatc aaagaagatc acaacaagaa aattcgcatc
     1261 aacgtggcaa tcaatcacat ctgacggaga cgactcatga cccagtcccc cgagcccttg
     1321 cccgctgcag acccggcgca gcccacgtcc ctggcccgct ccgtcgcgct gttcggcggg
     1381 gccgcgctgc tctgcgtcct cggctgcatc tgcgccgtga tgacggcgat gctcatggcc
     1441 caggcgcagg agcgcaccct ggcgtggatg gacgcacggg tggcctccgt cgctgattcg
     1501 ctggacacgc acgaccagac cgcgcgcctg ctcgtcgagc gcttcgccaa ggtgttcgcg
     1561 gaccagttcg gccgcaactt cgccctcgac gaggcggcgg gcaagctcag ccagctgggc
     1621 atcccgctca acgagcgcca cggcccgtgc gacaagttca aggagttcac cggcggggag
     1681 gccgcggtgc tgatggcgca gggcgggcgc ttcatggcga tcagcacctc gctggtcgat
     1741 gccaggggcg agcgctcgct cggctgggcg atcgggccag ggcatccggc gtacgccgcg
     1801 ttgcagaagg gccagacctg ggccggccgc ggccagttgc tcgggcgctc gttcgagcag
     1861 cgcgtgcagc ccgtgcgcga cctgcaggac cgcatcgtcg gcgcgctgta catcgccttc
     1921 gacctggcgg acttcgacag cgccatcgag cgccgcgtga acgccacgcg cttcttcgag
     1981 tcgggcgccg cgtacgtggt cgatcccggc cgcaacggcg aaccggcccg catcgtcgcg
     2041 ccggcgcgcc tgcgcg
//
LOCUS       DATCRO010000443         6873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_747588, whole genome shotgun sequence.
ACCESSION   DATCRO010000443 DATCRO010000000
VERSION     DATCRO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747588"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VIN75_12015"
     CDS             <1..523
                     /locus_tag="VIN75_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HEY8974939.1"
                     /translation="GGPPNAIDYWVICDSANCHLFFSADDGKLYRSKTSIDNFPNFSG
                     YETVMSDPMPGRLFEASNVYKVQGSGKYLLLVEAYGPRYFRSWTAASLDGPWTPLADQ
                     QSAPFAGQANVSFDGAAWTRDISHGEMIRAGYDETLAIDACNMQYLYQGVDPTSTAAY
                     DHLPYRLGLLRQR"
     gene            753..1799
                     /locus_tag="VIN75_12020"
     CDS             753..1799
                     /locus_tag="VIN75_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8974940.1"
                     /translation="MVDVARAAGVSEMAASSALNGGRRGSARVSAETRERVLAAAQRL
                     RYRPNATARALASQRTNAIGFVANFLGEEPNLYFLEVFSGVIQGAAMAGQTAAVFTLG
                     DWTEAATRIPAFCDGRVDGLILLGPRLKGDHLAWWPQHTPLVSVHADPLVSGMSNIES
                     DDEAGAYELVRHMIAMGHRRILHVAGPAGMAGADRRIEGYQRAHAAAGLKPAAGHVVR
                     APISVDGGREAMQQWLARRRGRAYPDAVFGFNDAVAIGCVEALRARGLRVPEDISVAG
                     YDHNLMARAVQLTTVRQPLHEMGRLAVEVLVRRIEALRQGEPDPGPANIVLPTELITG
                     ATLVRAPASRPTIA"
     gene            1941..3191
                     /locus_tag="VIN75_12025"
     CDS             1941..3191
                     /locus_tag="VIN75_12025"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY8974941.1"
                     /translation="MLQLVAALGRAGTVLRESCRRTLAVLVAGLFAVTASSFAHETLK
                     LDAPKPLLAPTPPMGWNSWNKFACDINEALVRKQADAMVASGMKAAGYQYIIIDDCWQ
                     KSRDENGDIVADPERFPSGIKALADDLHAKGLKFGIYSDAGALTCGGRPGSAGHEFQD
                     ARQYAKWGVDYLKYDWCYTGTRDAEAAYTIMAKALRESGRDIVLSICEWGNNHPERWA
                     APIGHLWRTTGDIFDGWKGTKDWSHGMTDILDFQVERWRDSGPNAWNDPDMLEVGNGG
                     MTSTEYESHFSLWAMLAAPLIAGNDLPAMSADTHRILTNADVIAVDQDVLGMQARRIW
                     KQGDLEIWARPLKGGDLAVVLFNRGAAPAGMTVDWALLNLPAGLRADVKDLWSKKVTR
                     RVHGDYGGTVAAHGVIMLRITPAL"
     gene            3221..5131
                     /locus_tag="VIN75_12030"
     CDS             3221..5131
                     /locus_tag="VIN75_12030"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY8974942.1"
                     /translation="MDKPSLLIACVFWALGATTAHGANLTVASPDGRAALLVADDGTT
                     FSVTRRGETVIAASPLGLDLDGEPALGALKLESRSDVAVDQVIPLTATKAAVARDHYR
                     GATFAFREAGGGRRILIDARVYDEGVAFRYRLDGPGPVNLRGERTAFALAGDPSCLAA
                     PVDGAHEASFERLHLSQLKEGVAYDVPLACATPSGRTHYAITQAQLQDYTGASLWRDG
                     AALRVHLSTVPGRQGPAVVSASGLTTAWRVVMMADRAGDLIESPLIGNLNPAPQGDFA
                     WVKPGKAAWDWWSGPLEGVKPDMATYQRFIDFAAESGFPYYLIDAGWSWGAGPCCEAR
                     PTTDIARAADGIDMPALVRHAADKGVGLLLWVHWQHLAPRMDEVLDTYARWGIKGIKV
                     DFMNRDDQEMVAFYQRAAAATAQRHLLLDLHGAYVPAGLQRTYPNFITQEGVLGAEWN
                     KMDRRVTPRHNLMLPYTRMLAGPMDYTPGGFRNATPQTFQVRAVLPQTQTTRGQVLAM
                     YVVYDSPLQMVADDPDAYRDAAGFDFVRRVPTSWDETRFIQGEPGRDIVLARRQGRAW
                     YVGAMAADEARAEHVPLRFLPPGKYRATLWQDGDSPTAVTRRELIVSRRDVLALELAA
                     AGGAAVILEPVP"
     gene            5356..>6873
                     /locus_tag="VIN75_12035"
     CDS             5356..>6873
                     /locus_tag="VIN75_12035"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY8974943.1"
                     /translation="MTQASRRAVLRSLAALSCVGGAALAEPARASVQAGVLQPFPLRD
                     VRLLDGPWRRAQVLAGRYLMSLDSDRLLHNFRVNAGLPPKAPVYGGWESQEPWVDIRC
                     HGHTLGHYLSACAMMGAATGEPAFRRRVDYIVGELLACQQAAGNGLVCAFPDGDVQLR
                     NGLAGRPVTGVPWYTMHKVMAGLRDAHVHGGSADALAVLRGLALWVQDAARGCDDQRF
                     QQLLDVEHGGMNEVLADLHALTGDERWLALARRFCHEALLRPLAEGRDTLDDLHANTQ
                     IPKVVGFARLNELTGEPRYGRAASYFWGRVAGARAFATGGHGDGEHFFPAAQVRQHLG
                     SAKTMETCGTYNMLKLTRALLLRDPQPAYADWYERALVNGILASQDPDTGMVTYFQPL
                     RPGYPKLYCTPEHSFWCCTGTGMESHAKYGDSIWFRDGDVLHLNQFVASELAWRERRL
                     RIRQVTAFPESAGSRIVVNSDAPTRFTLRLRHPAWCRTMRVSVNGTDRHESLEPGR"
BASE COUNT         1062 a         2406 c         2382 g         1023 t
ORIGIN      
        1 cggcgggccg cccaacgcga tcgactactg ggtcatctgc gacagcgcga actgccacct
       61 cttcttctcg gccgacgacg ggaagctcta tcgctcgaag acgtcgatcg acaatttccc
      121 caacttctcc ggctacgaga cggtgatgtc cgaccccatg ccgggccggt tgttcgaagc
      181 cagcaacgtg tacaaggtgc aaggcagcgg caagtacctc ctgctggtcg aggcctacgg
      241 cccgcgctat ttccgctcgt ggacggcggc cagcctggac ggcccgtgga cgcccctggc
      301 ggaccagcag tctgcgccgt tcgcgggcca ggccaacgtc tcgttcgacg gcgccgcctg
      361 gacccgcgac atcagccacg gcgagatgat ccgcgccggc tacgacgaaa ccctggcgat
      421 cgacgcttgc aacatgcagt acctctatca gggcgtcgac ccgacttcga ccgcggccta
      481 cgaccacctg ccgtaccggc tcgggttgct gcggcaacga tgatcatcgc gcgggcggac
      541 gggcggctgc cgtgagccat cggatgtctg cgccggcggc cgctcgaccg ccgtggcggg
      601 tcatggcgaa gtgaatcgcg acgcaggtgc tacagtgccc cgcgttgcaa tgtcctacag
      661 cagcaccgag acccagcctc gtggccacct caaagccgaa atcaccgtcc gcgcccaagc
      721 cgaagacccg gcgtggcggg aatgcgccga cgttggtcga cgtcgcgcga gccgcgggcg
      781 tgtcggaaat ggcggcgtcg tccgcgctca acggggggcg ccgcggttcg gcccgggtgt
      841 cggccgagac gcgcgagcgc gtgctggcgg cggcccagcg gctgcggtac cggcccaatg
      901 ccacggcgcg cgcgctcgcg agccagcgca ccaacgcgat cggcttcgtc gccaatttcc
      961 tcggcgagga accgaatctc tacttcctcg aggtgttcag cggcgtcatc cagggcgccg
     1021 ccatggccgg gcagacggcc gccgtgttca cgctcggcga ctggaccgag gcagcgacgc
     1081 gcatccctgc cttctgcgac gggcgtgtcg acgggctgat cctgctcggt ccgcgcctga
     1141 agggcgacca cctggcatgg tggccgcagc acaccccgct ggtgtcggtg cacgccgacc
     1201 ccttggtgag cgggatgtcg aacatcgagt cggacgacga ggcgggtgcg tacgaactgg
     1261 tccgccacat gatcgccatg ggccatcgcc gcatcctgca cgtcgccgga ccggcaggca
     1321 tggcgggagc ggatcgccgc atcgaaggct accagcgggc ccacgccgcc gccggcctga
     1381 agccggcagc cggccacgtc gtccgggcgc cgatcagcgt cgacggcggg cgcgaggcga
     1441 tgcagcagtg gctggcgcgg cgccgcggcc gcgcgtaccc ggatgcggtg ttcggcttca
     1501 acgacgcggt ggccatcggc tgcgtcgagg cgctgcgggc ccgtggcctg cgcgtgccgg
     1561 aggacatctc cgtcgccggc tacgaccaca acttgatggc acgcgcggtg caattgacga
     1621 cggtccgcca gccgttgcac gagatgggcc gcctggccgt cgaggtgctg gtgcgacgca
     1681 tcgaagcgct tcgccagggc gagcccgatc cggggcccgc caacatcgtc ctgcccacgg
     1741 aactcatcac gggcgcgacg ctggtacgcg cgcccgcgag ccggccgacc atcgcctgaa
     1801 tgcggcgcgc ccgcgcggtc gtcgcgaggc ggtaggtgtt ttccttgagt catttattcg
     1861 gtgaccgaac tattatgcga gccacatgat ttagcgccaa atcagcgcca ggaaacgtac
     1921 ccgtggtcga tcaccgtctc atgctccagc tcgtcgccgc ccttggccgc gccggcaccg
     1981 tccttcgcga atcatgccgg cgcacgcttg ccgtcctggt cgccgggctc ttcgccgtca
     2041 cggcctcctc gttcgcgcac gagaccctga agctcgatgc gcccaagccg ctgctcgcgc
     2101 ccacgccgcc gatgggctgg aactcgtgga acaagttcgc ctgcgacatc aacgaagcgc
     2161 tggtccggaa gcaggccgac gccatggtgg cctcgggcat gaaggcggcc ggctaccagt
     2221 acatcatcat cgacgactgc tggcagaaga gccgcgacga gaacggcgac atcgtcgccg
     2281 atcccgagcg cttccccagc ggcatcaaag ccctggcgga cgacctgcac gccaagggcc
     2341 tgaagttcgg catctactcg gacgccggcg cgctcacctg cggcggccgg ccgggcagcg
     2401 cgggccatga gttccaggac gcgcgccagt acgcgaagtg gggcgtcgac tacctcaagt
     2461 acgactggtg ctacaccggg actcgcgacg ccgaggcggc gtacaccatc atggccaagg
     2521 cgctgcgcga atcggggcgc gacatcgtgc tgtcgatctg cgagtggggc aacaaccacc
     2581 ccgagcgctg ggcggcgccc atcggccacc tctggcgtac cacgggcgac atcttcgacg
     2641 gctggaaggg cacgaaggac tggtcgcacg gaatgaccga catcctcgac ttccaggtgg
     2701 aacgctggcg cgactccggg cccaatgcct ggaacgatcc ggacatgctg gaggtcggaa
     2761 acggcggcat gacgagcacc gagtacgagt cgcacttctc cctctgggcg atgctggccg
     2821 cgccgctgat cgccggcaac gacctgcccg cgatgagcgc cgatacgcat cggatcctca
     2881 ccaacgcgga tgtcatcgcc gtggaccagg acgtgctcgg catgcaggca cggcgcatct
     2941 ggaagcaggg cgacctggag atctgggccc ggccattgaa gggcggggac ctggccgtgg
     3001 tgctgttcaa ccgcggcgct gcgccggccg gcatgacggt ggactgggcg ctgctgaacc
     3061 tgcctgccgg actgcgtgcc gacgtgaagg acctgtggag caagaaggtg accaggcgcg
     3121 tgcacggcga ctatggcggc acggtggccg cgcatggcgt gatcatgctc cgcatcacgc
     3181 cggcgctgta ggccgcatga acggacgccg gaccccgatg atggacaagc cctcgctcct
     3241 gatcgcctgc gtcttctggg cactcggcgc gacgactgcg cacggtgcga acttgacggt
     3301 cgcctcgccg gacggccgag ccgccttgct ggtcgccgac gacggcacga cgttcagtgt
     3361 cacgcgccgc ggcgagacgg tgatcgccgc ctcgcccctc gggctggacc tggatggcga
     3421 accggctctc ggcgcgctga agctcgaatc gcgttcggac gtggccgtcg atcaagtgat
     3481 cccgctgacc gcgaccaagg cggccgtcgc gcgtgatcac taccgcggtg cgacgttcgc
     3541 cttccgcgaa gcgggcggcg gccggcgcat cctcatcgat gcgcgggtct atgacgaggg
     3601 tgtcgccttt cgctacaggc tcgatgggcc gggccccgtg aacctgcgtg gcgagcggac
     3661 cgcattcgca ctcgcgggcg acccgtcctg cctggctgcg cccgtggacg gcgcgcacga
     3721 ggcgtcgttc gagcgcctgc acctctcgca gctgaaggag ggcgtggcct acgacgtgcc
     3781 gctggcctgc gcgacgccgt ccggccgcac gcactacgcc atcacgcagg cgcagctgca
     3841 ggactacacc ggcgcttcgt tgtggcggga cggcgctgcg ctgcgcgttc acctgtcgac
     3901 cgtgccgggt cgccagggcc cggcggtcgt ctcggccagc ggactcacga cggcctggcg
     3961 cgtcgtcatg atggccgatc gcgccgggga cctgatcgag tcgccgctga tcggcaacct
     4021 caatccggcg ccgcaaggcg atttcgcctg ggtcaagccg ggcaaggcag cgtgggattg
     4081 gtggtccgga ccgctggagg gcgtcaagcc cgacatggcg acctaccagc gcttcatcga
     4141 cttcgcggcc gagtccggct ttccctacta cctcatcgac gccggctggt cctggggtgc
     4201 cggcccgtgc tgcgaggcga ggcccaccac cgacatcgcg cgcgccgccg acggcatcga
     4261 catgccggcg ttggtgcgtc atgccgcgga caagggagtc ggcctgctgc tctgggtgca
     4321 ctggcagcac ctggcgccgc gcatggacga ggtgctcgac acctacgcgc gctggggcat
     4381 caagggcatc aaggtcgatt tcatgaaccg ggacgaccag gagatggtgg cgttctacca
     4441 gcgcgccgcc gctgccaccg cgcagcgcca cctgctgctt gacctgcatg gcgcctacgt
     4501 gccggcgggg ctgcagcgca cgtatcccaa cttcatcacc caggaaggcg tgctcggggc
     4561 cgagtggaac aagatggacc ggcgcgtcac gccgcgacac aacctcatgc tgccctacac
     4621 gcgcatgctc gcgggtccca tggactacac cccggggggc ttccgcaacg cgacgccgca
     4681 gacgttccag gtccgtgccg tgctgccgca gacccagacc acgcggggcc aggtcctggc
     4741 catgtacgtc gtgtacgaca gcccgctgca gatggtcgcg gacgatcccg atgcctatcg
     4801 cgacgcggcg ggcttcgact tcgtccgtcg cgtgccgact tcgtgggacg aaacgcgttt
     4861 catccagggc gagcccggcc gcgacatcgt gctggcacgg cggcaggggc gcgcctggta
     4921 tgtcggagcg atggccgccg acgaggcgcg ggcggagcac gtgccgctgc gcttcctgcc
     4981 gcccggcaag taccgcgcca ccctctggca ggacggcgac tcgccgactg ccgtgacgcg
     5041 gcgcgagctc atcgtgtcac ggcgcgacgt gctggcactg gagctggccg cggccggcgg
     5101 cgccgccgtg atcctcgagc cggtcccttg aagcgacgaa ccagctgagc attccgcgcc
     5161 accgagagga cagggctgca cggcacacgg gcggaaagag tggccatcca ggcgaccgcc
     5221 gcatgaagac tcacacggag acaagttcgt gagacggatg atcccgcggt cgacgcagac
     5281 cgcgttcctt gccttggcgg cgatcacgtg cgcggcggca cgtcgcgtcg gggccctgcc
     5341 tggcggagcg ccggcatgac gcaggcgagc cgtcgcgctg tcctgcgctc gcttgcggcc
     5401 ttgtcctgcg tgggcggtgc ggcgctggca gaaccggcgc gggcgtccgt gcaggccggc
     5461 gtgctgcagc cattcccgct gcgggacgtg cggctgctcg acggcccctg gcgtcgtgcg
     5521 caggtgctgg ccggtcgcta cctgatgtcg ctggacagcg accgcctgct gcacaacttc
     5581 cgcgtcaacg caggactgcc cccgaaggca ccggtgtacg ggggatggga atcgcaggaa
     5641 ccctgggtcg acatccgctg ccacggccac acgctgggcc actacctcag cgcctgcgcg
     5701 atgatgggcg ccgccaccgg cgaacccgcc ttccgtcgtc gcgtggacta catcgtcggc
     5761 gagttgctgg cgtgccagca ggcggcgggc aacggcctcg tgtgcgcctt cccggacggc
     5821 gatgtccagc tgcgcaacgg cctggccggc aggccggtca ccggggttcc ctggtacacg
     5881 atgcacaagg tcatggccgg cctgcgcgat gcgcacgtgc atggcggcag cgcggacgcg
     5941 ctggccgtgt tgcgcggtct cgcgctatgg gtgcaggacg ccgcccgcgg ttgcgacgat
     6001 caacgcttcc agcaactgct cgacgtcgag catggcggca tgaacgaggt gcttgcggac
     6061 ctgcatgcgc tgaccggcga cgaacgctgg ctggccttgg ccaggcgctt ctgccatgag
     6121 gccttgctcc gcccgctcgc agaaggccgt gacaccctgg acgacctgca tgcgaacacg
     6181 cagatcccga aggtggtggg cttcgcgcgc ctgaacgaac tgacgggcga gccgcgctac
     6241 ggccgtgcgg caagctactt ctggggccgc gtggccggcg cgcgagcctt cgccaccggc
     6301 ggccacgggg acggggagca cttctttccc gccgcccaag tacgtcagca cctcggctcg
     6361 gcgaagacga tggagacctg cggcacgtac aacatgctga agttgacgcg cgccctgctg
     6421 ctgcgcgacc cgcagccggc gtatgcggac tggtacgaac gcgcgctggt caacggcatc
     6481 ctcgcgtcgc aggatccgga caccggcatg gtcacctact tccagccgct tcggcccgga
     6541 taccccaagc tgtattgcac gccggagcat tcgttctggt gctgcaccgg cacgggcatg
     6601 gaaagccatg ccaagtacgg cgactcgatc tggttccgcg acggcgacgt gctccatctg
     6661 aaccagttcg tcgcatccga gctggcatgg cgcgagcggc gcctgcgcat ccggcaggtc
     6721 actgcctttc ccgagagcgc cggatcgcgc atcgtcgtga attccgacgc gccgacccgc
     6781 ttcacgctgc ggctgcgcca ccccgcctgg tgccggacga tgcgggtcag cgtcaacggc
     6841 acggacaggc acgaaagcct cgagcccggc cgc
//
LOCUS       DATCRO010000444         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_252049, whole genome shotgun sequence.
ACCESSION   DATCRO010000444 DATCRO010000000
VERSION     DATCRO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252049"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VIN75_12040"
     CDS             <1..249
                     /locus_tag="VIN75_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY8974944.1"
                     /translation="SAPDPWYILPIVMTATSLFQTWLNPTPPDPMQAKLMWIMPLAFS
                     VMFIFFPAGLVLYWITNNVLSIAQQWFINKRLGVLGTK"
     gene            334..1740
                     /gene="mnmE"
                     /locus_tag="VIN75_12045"
     CDS             334..1740
                     /gene="mnmE"
                     /locus_tag="VIN75_12045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY8974945.1"
                     /translation="MLARTSDPIVAIATASGRGAVGIVRVSGARLAPLVDAICGRALK
                     PREATYLPFRDAEGEPVDHGLAIHFPSPHSFTGEDVLELQAHGGSVVLQLLLARCLEA
                     AAEPDPVTGRPRLPGLRVAEPGEFSQRAFLNGKIDLAQAEAIADLIDASTEAAARSAG
                     RSLSGAFSREIHTLRDALIHLRMLVEATLDFPEEEIDFLQKADAAGQLAKLQSQLAAV
                     QQRARQGALLREGIKVVIAGQPNAGKSSLLNALAGAELAIVSAVAGTTRDVVSQTIQI
                     HGVPLHVADTAGLRESSDEVEQIGVARAWGQIESADAVLFLHDLTRADQPDYAAADAE
                     ILAGLQRKLPASVPVLDVWNKQDAAPDTRPAQGIALSAKTGLGIEALREQLLAMAGWQ
                     AVPEGVYLARARHVQALARVETHLALAADHLAAQAQLLDLLAEELRLAQNALNEITGE
                     FGADDLLGVIFSRFCIGK"
     gene            1843..2304
                     /locus_tag="VIN75_12050"
     CDS             1843..2304
                     /locus_tag="VIN75_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8974946.1"
                     /translation="MSIQNLSRAIAENINTDAFALTFSVQQWETLAGYLQPIDTGVGD
                     VLIEQGTPDRSVFFVESGAISVHRVSSKEQMKLAVLTAGSVVGEGSFFSRQPHSANVV
                     VTGAGRVWRLTAIRFGELGNRQPNMALEIAMALGGVIAKRMAHRSKRVAVT"
     gene            2411..>2898
                     /locus_tag="VIN75_12055"
     CDS             2411..>2898
                     /locus_tag="VIN75_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY8974947.1"
                     /translation="MASICPVCSTENRDGANFCRSCGIKLSAAGDRIPPPPTPEREWA
                     TTAPAQLRAPTIPAPLQEIPPAPTPARPSFFSRSPASAPRHDDDEQTVLMLHDDGSPR
                     VPAPPAAPQGLESSWALSRERSKKPRVRIRKPAAVDRAPRWRAILLWVGLLLVAVAMI
                     VAG"
BASE COUNT          462 a         1042 c          987 g          407 t
ORIGIN      
        1 tcggcacccg acccctggta catcctgccg atcgtgatga cggccacgtc gctcttccag
       61 acctggctca acccgacgcc gcccgatccg atgcaggcca agctgatgtg gatcatgccg
      121 ctcgcgttca gcgtgatgtt catcttcttc ccggccggcc tggtgctgta ctggatcacg
      181 aacaacgtgc tgtcgatcgc gcagcagtgg ttcatcaaca agcggctggg cgtgctcggc
      241 accaagtgac ggcggcagta gccgcacggc actccagcca tccttcgaag ggccgcgtca
      301 gcggcccttt ttgctgaaag aatccgcacc accatgctcg cccgcacctc cgatcccatc
      361 gtcgccatcg ccaccgcctc ggggcgcggc gcggtcggca tcgtgcgcgt gtcgggcgcg
      421 aggctggcgc cgctggtcga cgcgatctgc ggccgcgcgc tcaagccgcg cgaggccacc
      481 tacctgccgt tccgcgacgc cgagggcgag cccgtcgacc acggcctggc catccacttt
      541 ccctcaccgc actccttcac gggcgaagac gtgctcgaac tgcaggccca cggcggatcg
      601 gtggtgctgc agctgttgct ggcgcgctgc ctggaggcgg cggccgagcc cgacccggtc
      661 accggccgcc cccgcctgcc cggcctgcgc gtggcggagc ccggcgaatt cagccagcgc
      721 gcctttctca acggcaagat cgacctggcg caggccgagg ccatcgccga cctgatcgac
      781 gccagcaccg aggcggccgc gcgcagcgcc gggcgatcgc tgtcgggcgc cttctcgcgc
      841 gagatccata cgctgcgcga cgcgctgatc cacctgcgca tgctggtgga ggccacgctc
      901 gactttcccg aggaggaaat cgacttcctg cagaaggccg acgcggccgg gcagctggcg
      961 aagctgcagt cgcagctggc cgccgtgcag cagcgcgcgc gccagggcgc cctgctgcgc
     1021 gagggcatca aggtcgtgat cgcgggacag cccaatgcgg gcaagagctc gctgctcaat
     1081 gcgctggccg gggcggagct ggccatcgtg agcgcggtgg cgggcacgac gcgcgacgtc
     1141 gtctcgcaga ccatccagat ccacggcgtg ccgctgcacg tggccgacac cgccggcctg
     1201 cgcgagagca gcgacgaggt cgagcagatc ggcgtggcgc gcgcctgggg gcagatcgag
     1261 agcgccgatg cggtgctgtt cctgcacgac ctgacgcgcg cggaccagcc cgactacgcc
     1321 gccgccgacg cggagatcct ggccggcctt cagcgcaagc tgcccgcgag cgtgccggtg
     1381 ctcgacgtct ggaacaagca ggacgcagcg ccggacaccc ggccggcgca gggcatcgcc
     1441 ctctccgcca agaccggcct gggcatcgag gccctgcgcg agcagctgct ggccatggcg
     1501 ggatggcagg ccgtgcccga gggcgtgtac ctcgcgcgtg cgcgccacgt gcaggcgctg
     1561 gcgcgggtcg agacgcacct ggcgctggcc gccgaccacc tcgcggcgca ggcgcaactg
     1621 ctcgacctgc tggccgagga gctgcgcctg gcgcagaacg ccctgaacga gatcaccggc
     1681 gagttcggcg cggacgacct gctgggcgtc atcttttcgc gcttctgcat tggcaaatag
     1741 ccacagacgg cggcggccga cctccaaaaa acgtaaacag ttgtagcgaa ataacatggc
     1801 catggcggat acttcagccc acatgcgggg gccgagaaac acatgtccat ccagaacctg
     1861 agccgcgcca tcgcggagaa catcaacacc gacgcgttcg cgttgacgtt cagcgtccag
     1921 caatgggaaa cgctggcggg ttacctgcag ccgatcgaca ccggcgtcgg cgacgtgctg
     1981 atcgaacagg gcacgcccga ccgctcggtg ttcttcgtcg agagcggcgc catcagcgtg
     2041 caccgcgtca gcagcaagga acagatgaag ctggccgtgc tcaccgcggg gtcggtggtc
     2101 ggcgaaggtt cgttcttctc gcggcagccg cattcggcca acgtggtggt caccggcgcg
     2161 ggccgagtct ggcggctcac ggcgatccgc ttcggcgaac tgggcaaccg ccagcccaac
     2221 atggcgctgg agatcgcgat ggcgctgggc ggcgtgatcg ccaagcgcat ggcgcaccgt
     2281 tccaagcgcg tcgcggtgac ctgagcagga agcttcctct cccgcctgcc gccggatcga
     2341 tcctgcggat gcgggccagc caccgccgtc caagaccccc gaaaaacaag aagtagtcag
     2401 caagcaatcc atggcctcga tctgtccggt ctgcagcacg gagaaccgtg acggcgcgaa
     2461 tttctgcagg agctgcggca tcaagctgtc cgccgcggga gaccggattc ctccgccgcc
     2521 gacccccgaa cgcgaatggg ccaccaccgc acccgcccag ctccgcgccc cgaccattcc
     2581 tgcgccgctc caggagatcc cgccggcccc gacgccggcc cgcccgtcgt tcttcagcag
     2641 gtcgccggcc tccgcgccgc ggcatgacga cgacgagcag accgtcctca tgctgcacga
     2701 cgacggctcg ccgcgcgtgc cggcgccgcc cgcggcgccg caggggctgg aatcgagctg
     2761 ggcgctgtcg cgcgagagat cgaagaagcc ccgcgtcagg atccgcaagc ccgctgcggt
     2821 cgaccgggcg ccgcgctggc gggcgatcct gctgtgggtc ggcctgctgc tggtggcggt
     2881 ggcgatgatc gtggcggg
//
LOCUS       DATCRO010000445         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_859941, whole genome shotgun sequence.
ACCESSION   DATCRO010000445 DATCRO010000000
VERSION     DATCRO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859941"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VIN75_12060"
     CDS             complement(<1..369)
                     /locus_tag="VIN75_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8974948.1"
                     /translation="MNATLRQHAETQFAEELAELRRQDNRARPANWTLSPWAVVRYLM
                     GGRLDNGFEVSAKYIGDARLMEIAVATLATDRALLLYGVPGTAKSWVSEHLAAAISGD
                     STLLIQGTAGTGEEQLRYGWN"
     gene            complement(393..1985)
                     /locus_tag="VIN75_12065"
     CDS             complement(393..1985)
                     /locus_tag="VIN75_12065"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HEY8974949.1"
                     /translation="MTQDVGAWSPLLPVAMVGTDRHTAPMPRWPGAIGDAIAALGEPA
                     AGQAATGVLRAAAILAVCGAAGARPGPSPQAPGVPAGRESTPAVDDPELLRSLGWTMV
                     DGPERLKQAFCLRLGQAGLRLPPVLLTVALEQARQSLAMRPLVQPLLGERGVWLARQR
                     DDWRFAAGVAAAEDDDPRQWDEGTLEQRRAFLAAERARDPRAARERLAAALPELPAKE
                     RAELARGLADGLSMDDEPLLEQLRNDRGQDVRAVALDLLLRLPAAAHPRRAAARVAAL
                     MARGGLLTGRRWNIEPPSEAGTDWRADQVDPDVPKGAMGQRAWWLYQLVRQVPLGWWT
                     MHTGLSPRELSAWAQAGDWGEPLWRGWRDVLRRAPDQAWAELLLDAKGPAGYGGGSIQ
                     GDRELALGQVCAEARERWVVQQLATSDEPLSATLWTVMARCAPGESISRNLAAPIVQR
                     VRRTLALDTTVAPGTHNANVLEQEHVGRALPDLCCTLPLPALADFDDWAASPSQSPAL
                     ARARHAGRQIIQARRVLASRLP"
     gene            complement(1982..3331)
                     /locus_tag="VIN75_12070"
     CDS             complement(1982..3331)
                     /locus_tag="VIN75_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY8974950.1"
                     /translation="MTLTVEEVTALAPDDASAKAARGLVAPAKWPVLGANDAAAWGEC
                     QGSGSKPYQTQVDLSGPAFRCSCPSRKFPCKHGLALLLLRAEHGARFASGEAPAWVAE
                     WLASRSEKAQRKEAQQAEKAATPVDPAAAARREAQRWTRIEGAARDLQRFLADQLARG
                     LASLDANTLKTWATLSARMVDAQAPGLGARLQEVAAGVRQGEHWPERTLQGLGLLQLA
                     CDAFARRESLSPAQQADLRTLAGWPHDQAEVTAGGERMADRWLVLGVATEERPNKLTE
                     RRVWLHGTGTGRAAWLLDHAFAGRGFEQAWMPGTVVDATMAYFPGAGPLRALAVERGR
                     SVEARDAGSLVESAAQAAASDPWGVVAARVAANPWTTLHPLLLRDAVVAVSDGQAWMH
                     QAGAAWPVQASSGELWLLLACTGGAPVTLAAEWDGTGMRPLTAWSEGGHLAWIKEAA"
     gene            complement(3433..3975)
                     /locus_tag="VIN75_12075"
     CDS             complement(3433..3975)
                     /locus_tag="VIN75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8974951.1"
                     /translation="MAPVHGSRNGGSPLDRLSAWHRLGERQFEVMRWLASGRATPPDA
                     VRNTAQGVLRYFDLSVTVHHRDEEDDLHPALVESMAGSDPVCLREMAEAATLAHRAIE
                     AAWRTLRPAFVALARAEPARVDPAAVDVLVAACEHSFAHEDRELLPTAHRLLDDDTLR
                     QLRARIDERHYKGRGNATSA"
     gene            complement(4089..5219)
                     /locus_tag="VIN75_12080"
     CDS             complement(4089..5219)
                     /locus_tag="VIN75_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEY8974952.1"
                     /translation="MGQRNVAQWLAAFVPAPVTVSRREIALGALGALLGLASAAWLSR
                     HMLAGLNPWFIAPMGASAVLLFAVPSSPLAQPWSIIGGNLVAALIGVTCARWVPDAAL
                     AGALAGALAIAAMFSLRCLHPPSGAVALTAVLGGPAVRALGYAFVLWPVAIDSLLLLM
                     AALLFNVSARRRYPHRAPAPAPTHLTRDAPPSARVGLALEDLHAALEARGELLDVSED
                     DLQELFVEAEHRAWRRRFGSLRCNEVMSHDVITVTPSTAAQDAWQRMIHHAVKALPVV
                     DAQSRLLGIVTLHDFFVGHEVATQAGHAAWLVGDIMTRDVLTASPAQELVDLMHAFSD
                     GGRHHLPVVDHDRRLVGMLTQSDMVAALFRAGIERPAAALAH"
     gene            complement(5221..>5558)
                     /locus_tag="VIN75_12085"
     CDS             complement(5221..>5558)
                     /locus_tag="VIN75_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8974953.1"
                     /translation="AGLARPDVARVADLFGANVPAMALADENCREIATRLAVHGLERL
                     PVVTDAQSRRLIGIVSRSDLIKPSLAIGEEELHAERFFEPPLGLARARFRALTEVSQP
                     PELDDDEDA"
BASE COUNT          700 a         2068 c         2116 g          674 t
ORIGIN      
        1 gttccagccg tagcgcaact gctcctcgcc cgtgcccgcc gtgccctgga tcagcagcgt
       61 cgagtcaccg ctgatggccg cggccaggtg ctccgagacc caggacttcg cggtgcccgg
      121 cacgccgtac agcagcagcg cgcggtcggt ggccagcgtg gcgacggcga tctccatgag
      181 ccgcgcgtcg ccgatgtact tggcgctgac ctcgaagccg ttgtccagcc gcccgcccat
      241 gaggtagcgc acgacggccc acggcgacag cgtccagttg gcgggccgcg cacggttgtc
      301 ctggcggcgc agttcggcca gttcctcggc gaactgggtc tcggcatgct ggcggagcgt
      361 ggcgttcagg gcagtggtca tggcgggagg cttcagggaa ggcgggaggc caggacgcgg
      421 cgcgcctgga tgatctggcg gcccgcgtgg cgggcgcggg cgagggcggg ggactggctg
      481 ggcgaggccg cccagtcgtc gaagtcggcg agcgcgggca gggggagcgt gcagcacagg
      541 tccggcaggg cgcggccgac gtgttcctgc tcgaggacgt tggcgttgtg cgtacccggc
      601 gccacggtcg tgtccagcgc cagcgtgcgc cgcacgcgct gcacgatggg ggcggccagg
      661 ttgcgcgaga tgctctcgcc cggcgcgcag cgcgccatga ccgtccagag cgtcgcggac
      721 agcggctcgt ccgatgtggc cagctgctgc acgacccagc gctcgcgcgc ctcggcgcag
      781 acctggccga gcgcgagttc gcgatcgccc tggatcgatc ctccgccgta gccggccggt
      841 cccttggcgt ccagcagcag ctcggcccag gcctggtcgg gcgcgcggcg cagcacgtcg
      901 cgccagccgc gccagagcgg ctcgccccag tcgccggcct gcgcccaggc cgacagctcg
      961 cgcggcgaca agcccgtatg catcgtccac cacccgagcg gcacctggcg caccagttgg
     1021 tagagccacc acgcgcgctg acccatcgcg cccttgggga cgtccgggtc gacctggtcg
     1081 gcccgccagt cggtgccggc ctcggacggc ggttcgatgt tccagcgccg tccggtgagc
     1141 aggccaccgc gcgccatcag cgccgcgacg cgggccgcgg cgcggcgcgg gtgtgccgcg
     1201 gcgggcaggc gcagcagcag gtccagcgcc acggcgcgca cgtcctggcc gcggtcgttg
     1261 cgcagctgtt cgagcagcgg ttcgtcgtcc atcgacaggc cgtccgccag gccgcgcgcc
     1321 agctccgcgc gctccttggc cggcagctcg ggcaacgcgg cggcgaggcg ttcgcgcgcc
     1381 gcccgcgggt cgcgcgcccg ctccgccgcg aggaaggcgc ggcgctgttc gagcgtgcct
     1441 tcgtcccatt ggcgcggatc gtcgtcctcg gccgccgcca cgccggcggc gaaacgccag
     1501 tcgtcgcgct ggcgggcgag ccagacgccg cgctcgccca ggaggggctg caccagcggc
     1561 cgcatcgcca gcgactggcg cgcctgttcc agcgccacgg tcagcaggac gggcggcagc
     1621 cgcaggccgg cctgcccgag gcgcaggcag aacgcctgct tcaggcgctc gggcccgtcg
     1681 accatcgtcc agccgaggct tcgcagcagt tcgggatcgt ccacggccgg cgtgctctcg
     1741 cgtccggccg gcacgcccgg cgcctgcggg ctcggccccg gccgtgcccc ggccgcaccg
     1801 cacaccgcga ggatggcggc ggcgcgcagg acgcccgtgg cggcttggcc ggccgcgggt
     1861 tcgccaagcg ccgcgatcgc gtcgccgatg gcgccgggcc agcgcggcat cggcgccgtg
     1921 tggcgatccg tgccgaccat ggccacgggc agcagaggcg accaggcgcc gacgtcctgc
     1981 gtcacgcggc ctccttgatc caggccagat ggccgccttc gctccatgcg gtcaacggcc
     2041 gcatgccggt gccgtcccat tcggccgcca gcgtcaccgg cgcgccgccc gtgcaggcca
     2101 gcagcagcca cagctcgccg gacgacgctt gcaccggcca cgccgcgccg gcctggtgca
     2161 tccaggcctg gccatcgctc acggcgacga cggcgtcgcg cagcaacagc gggtgcaagg
     2221 tcgtccacgg attggccgcg acgcgcgcgg caaccacgcc ccacgggtcg gacgcggccg
     2281 cctgcgccgc ggactcgacc agcgagcccg cgtcgcgcgc ctcgacggac cggccgcgct
     2341 cgacggcgag cgcgcgcagc gggccggcgc cggggaaata cgccatcgtc gcgtcgacca
     2401 ccgtgccggg catccacgcc tgctcgaagc cacggcccgc gaaggcgtgg tcgagcagcc
     2461 aggccgcgcg gcccgtgccc gtgccgtgca gccagacgcg gcgctccgtg agcttgttgg
     2521 gccgttcctc ggtggccacg cccaggacca gccagcgatc ggccatgcgc tcgccgccgg
     2581 cagtcacctc ggcctggtcg tgcggccagc cggccagcgt gcgcaggtca gcctgctgcg
     2641 ccggcgagag cgattcgcgg cgcgcgaacg cgtcgcacgc gagctgcagc aggcccaggc
     2701 cctgcaaggt gcgctcgggc cagtgctcgc cctggcgcac gcccgcggcg acctcctgca
     2761 ggcgcgcgcc caggccgggt gcctgcgcat cgaccatgcg tgccgacagc gtggcccagg
     2821 tcttgagcgt gttcgcgtcg agcgaggcca ggccgcgcgc gagctggtcg gccaggaagc
     2881 gctggaggtc ccgcgcggcg ccctcgatgc gcgtccagcg ctgcgcttca cgcctggcgg
     2941 cggcggccgg atcgacgggc gtggccgcct tctcggcctg ttgcgcttcc ttcctctggg
     3001 ccttctcgct gcgcgaggcg agccattcgg cgacccaggc cggcgcctcg ccgctcgcga
     3061 agcgcgctcc gtgctcggca cgcagcagca acagggccag tccatgcttg caggggaact
     3121 tgcggctcgg gcaggagcag cggaaggccg gccccgacag gtcgacctgg gtctggtacg
     3181 gcttggagcc gctgccctgg cactcgcccc aggcggccgc gtcgttggcg cccagcacgg
     3241 gccacttggc gggcgccacg agaccgcggg ccgccttggc ggacgcgtca tcgggcgcga
     3301 gtgcggtgac ttcctccacg gtcagcgtca tgctggcctg tctccctggc atcgttgtgc
     3361 ccgcagtcta cgggacagcg acgacggtcc cgcgccgccg ttccggggat ttccaccaat
     3421 tcggcgcggc gctcaggccg aggtggcgtt gccgcggccc ttgtagtggc gctcgtcgat
     3481 gcgcgcgcgc agttgccgca gcgtgtcgtc gtccaggagg cggtgcgccg tgggcagcag
     3541 ctcgcgatcc tcgtgggcga agctgtgctc gcacgcggcg accagcacgt cgaccgccgc
     3601 cggatcgacc cgcgccggct cggcgcgcgc gagcgcgacg aacgcgggac gcagcgtgcg
     3661 ccacgccgcc tcgatcgcac ggtgcgcgag cgtggccgcc tcggccatct cgcgcaggca
     3721 cacgggatcg gatccggcca tcgattcgac cagcgccgga tggaggtcgt cctcctcgtc
     3781 gcggtggtgc accgtgacgg acaggtcgaa gtagcgcagc acgccttgcg cggtgttgcg
     3841 cacggcgtcg ggcggcgtgg cgcggccgga cgcgagccag cgcatcacct cgaactggcg
     3901 ctcgcccagg cggtgccagg cggacagccg atcgagcggg gagccgccgt tccgggagcc
     3961 gtgcacggga gccatggcgc gggcgcggcg tcagcggccc gctgcctggc cgcgcgcccg
     4021 cgccgcggtg cacacggcgg cgggcagaga gggcagggac gggcgggcgg gcacgatcgg
     4081 atcgggggtc agtgggcgag ggcggctgcc gggcgctcga tgccggcgcg gaacagggcc
     4141 gcgaccatgt cggactgcgt cagcatcccc accaggcggc ggtcatggtc gacgaccggc
     4201 aggtgatggc ggccgccgtc cgagaacgca tgcatgaggt cgaccagttc ctgcgcgggc
     4261 gaggccgtca gcacgtcgcg ggtcatgata tcgccgacca gccatgccgc gtggccggcc
     4321 tgcgtggcga cctcgtggcc gacgaagaag tcgtgcagcg tgacgatgcc cagcaggcgc
     4381 gactgcgcgt cgaccaccgg cagcgccttg acggcatggt ggatcatgcg ctgccaggcg
     4441 tcctgcgccg ccgtggaggg cgtcacggtg atgacgtcgt gcgacatgac ctcgttgcag
     4501 cgcaggctgc cgaagcggcg tcgccacgcg cggtgctcgg cctcgacgaa cagctcctgc
     4561 aggtcgtcct cgctgacgtc gagcagttcg ccgcgcgcct cgagcgccgc gtgcaggtcc
     4621 tccagcgcca gccccacgcg tgcgctgggg ggcgcgtcgc gcgtgaggtg cgtgggcgcc
     4681 ggcgcgggcg cgcgatgcgg gtagcgccgg cgcgccgaca cgttgaacag cagcgcggcc
     4741 atcagcagca gcagcgagtc gatggccacc ggccagagca cgaaggcata gccgagcgca
     4801 cgcacggccg gcccgcccag cacggcggtc agcgccaccg cgccgctggg cgggtgcagg
     4861 cagcgcagcg agaacatcgc cgcgatggcc agcgccccgg ccagggcgcc ggccagcgcg
     4921 gcgtcgggca cccagcgcgc gcaggtgacg ccgatcagcg cggccaccag gttgccgccg
     4981 atgatggacc agggctgcgc gagcgggctg gacggcacgg cgaacagcag caccgccgac
     5041 gcgcccatcg gcgcgatgaa ccacgggttc aggccggcca gcatgtgccg gctcagccag
     5101 gccgcgctgg ccaggcccag cagcgcgccc agcgcgccca gcgcgatctc gcgccgcgac
     5161 acggtgacgg gcgccgggac gaaggccgcg agccactgcg cgacgttgcg ttgccccatc
     5221 tcaggcgtcc tcgtcgtcgt cgagctccgg cggctgcgac acctcggtca gcgcgcggaa
     5281 gcgggcgcgg gccaggccca gcggcggctc gaagaagcgc tcggcgtgca gttcctcctc
     5341 gccgatcgcc agcgacggct tgatcaggtc gctgcgcgag acgatgccga tcagccggcg
     5401 cgactgcgcg tcggtgacca ccggcaggcg ctcgaggccg tgcacggcca ggcgtgtcgc
     5461 gatctcgcgg cagttctcgt cggccagggc catcgccggc acgttcgcgc cgaacaggtc
     5521 cgccacgcgc gcgacgtcgg gcctggccag gccggccg
//
LOCUS       DATCRO010000446         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_893612, whole genome shotgun sequence.
ACCESSION   DATCRO010000446 DATCRO010000000
VERSION     DATCRO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893612"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1066
                     /locus_tag="VIN75_12090"
     CDS             344..1066
                     /locus_tag="VIN75_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8974954.1"
                     /translation="MLTLRKAQDRGHADHGWLNSFHSFSFADYHDPAHMGFGNLRVVN
                     DDTIAPGTGFGTHGHRDMEIISYVLDGALAHQDSMGNGTAGGANAGVIKPGDVQRMTA
                     GTGVRHSEFNHSKDTPTHFLQLWILPERGGLAPGYEQVSVPEASKRGKLALVGSPDKV
                     AGAVTLNADARLFAGLFDGDERQELPLPAGRLTYVHVARGSLRVNGQQLGAGDALKIR
                     DEAALVLEGGDKAEVLVFDLKP"
     gene            1133..2392
                     /locus_tag="VIN75_12095"
     CDS             1133..2392
                     /locus_tag="VIN75_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEY8974955.1"
                     /translation="MTTPSKRAYSANTRLLHADRLGGVEHGAIHKPLHLSAAFNYPKA
                     QDLVDVFQGNQAGHVYARQGNPTVNALEAKVTLLEDAKGSATFATGMAAITAVFLALL
                     RAGDHLVCTRFLFGNTNSLAQTLQALGVGVSFVDATDAANVRAAITPATRMVFVETIA
                     NPVTQVADLEGIGQACRDAGLLYVVDGTLTTPLLVRGRDIGAGLVVHSLSKSIGGHGN
                     ALGGAVVDTGLFDWSAYANILPPYKKGPPDKWGLLQVRKKGLRDMGGTLRAEDAHRIA
                     VGAETLQLRMRRVCDNALALARWLEQQPAIARVYYPGLASHAQHERAARLFKGDFGLL
                     MSFELREGIDTLAFLDALQLVIVSSHLADNRTLAIPVAQTIYHEMGPQRRAEMGIADG
                     LIRLSVGIEDTADLIDDFTRALQAVTR"
     gene            complement(2576..3478)
                     /locus_tag="VIN75_12100"
     CDS             complement(2576..3478)
                     /locus_tag="VIN75_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8974956.1"
                     /translation="MAEPLSELNALVARHARPGAPATALAGVRATISRVPTEAIHCVT
                     EPSLGLVVQGAKRTIVGDRVFEYGAGDFVVTSVDLPIASRIVRASADEPYLACGLSLN
                     PATIASLLLETPGADELVATPCGMGVHAAPVELVEAMVRLLRLLDAPRDAPVLRPLIE
                     REILWRLLRGPQGGRVRQFGLADSRLAQVSHAIRHIRQNFAEPLSVEQLAQVATMSES
                     SFHRHFRAVTAMTPIQYQKQIRLQEARARLMADSGDVAAVGYAVGYDSASQFSREYSR
                     MFGAPPGRDVARLRLVAPLERSAA"
     gene            3636..4478
                     /locus_tag="VIN75_12105"
     CDS             3636..4478
                     /locus_tag="VIN75_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY8974957.1"
                     /translation="MSKTWFITGATRGLGADIAAAALKAGDRVVATGRQRAAVSDRLG
                     PDSDRLLSLSLDVNDPAQAQAAAAAAVERFGAIDVLVNNAGYGHIGFFEESSPADIEA
                     QFATNVFGLLNVTRAVLPAMRAARRGHVFNLSSTAGIRGIAAGSLYCATKFAVEGFSE
                     SLAQELAPFGIHVTIVEPGPFRTDFLTPESLKFTAAEVADYDARRAEMRASFEQRNGK
                     QAGDPVRLAQAIVTLSKEAAPPMRFAAGAMAVSALDAKVASLKAELDRWRELAIGTDY
                     PAGT"
BASE COUNT          730 a         1693 c         1601 g          636 t
ORIGIN      
        1 ggcccgcggc aaggccaaga tgagtgccct cggcgccgcc atgcataagc tcgcgcaatt
       61 gtgcttcggc gtcgttcaca caggccagcc ctatcgcgct gattggcctg tcaaggcttg
      121 acgcccgaga cggtatctac gaacgcgccg gcattcgtgc catgtagggc gggttggccg
      181 caggcgtaac ccgccagttc gacaaccctg gtcgccccgg gatgacaagg caccccgtct
      241 caacgctcca ttcgaatcgc ctgcatcctc gacaccccgc cgcgccccac acttctcctc
      301 acgccgtcag cggcatcgct gacatcttct ggagaaacga ctcatgctca cgcttcgcaa
      361 ggcccaggac cgcggtcacg ccgaccatgg ctggctcaac agcttccaca gcttctcgtt
      421 cgccgactac cacgacccgg cgcacatggg cttcggcaac ctgcgcgtgg tcaacgacga
      481 caccatcgcc ccgggcaccg gcttcggcac ccacggccat cgcgacatgg agatcatcag
      541 ctacgtgctg gacggcgcgc tcgcgcacca ggactccatg ggcaacggca ccgccggcgg
      601 cgccaatgcc ggcgtgatca agccgggcga cgtccagcgc atgaccgccg gcaccggcgt
      661 gcgccacagc gagttcaacc actccaagga cacccccacg cacttcctgc agctgtggat
      721 cctgcccgag cgcggcgggc tggcgccggg ctacgagcag gtcagcgtgc ccgaggcgtc
      781 caagcgcggc aagctggcgc tggtcggctc gcccgacaag gtggccggcg cggtcacgct
      841 caacgccgac gcgcgcctgt tcgccggcct gttcgatggc gacgagcgcc aggagctgcc
      901 gctgcccgcc ggccggctga cctacgtgca cgtcgcgcgc ggcagcctgc gggtcaacgg
      961 ccagcagctg ggcgccggcg atgcgctcaa gatccgcgac gaggcggcgc tggtgctcga
     1021 gggcggggac aaggccgagg ttctcgtgtt cgacctcaag ccttgatcgg acaagaaggc
     1081 gggggccgcc cgtctgcatg agaatgggac tcgaaccacc gagtcccttc gcatgaccac
     1141 gccctccaag cgcgcctaca gcgccaacac ccgcctgctc cacgccgacc gcctcggcgg
     1201 cgtcgagcat ggcgcgatcc acaagccgct gcacctgtcg gccgcattca actacccgaa
     1261 ggcgcaggac ctggtcgacg tgttccaggg caaccaggcc ggccacgtct acgcccgcca
     1321 gggcaatccc accgtcaacg cgctcgaggc gaaggtgacg ctgctggagg acgccaaggg
     1381 ctccgcgacg ttcgccaccg gcatggccgc catcaccgcg gtgttcctgg cgctgctgcg
     1441 cgcgggcgac cacctcgtgt gcacgcgctt cctgttcggc aacaccaaca gcctggcgca
     1501 gacgctgcag gcgctgggcg tgggcgtcag cttcgtcgac gcgacggacg ctgccaacgt
     1561 gcgcgccgcc atcacgccgg ccacccgaat ggtgttcgtc gagaccatcg ccaacccggt
     1621 gacccaggtg gccgacctcg agggcatcgg ccaggcctgc agggacgccg gcctgctcta
     1681 cgtcgtcgac ggcacgctca ccacgccgct gctggtgcgc ggccgcgaca tcggcgccgg
     1741 cctcgtcgtt cactcgctga gcaagagcat cggcggccac ggcaacgcgc tcggcggcgc
     1801 cgtcgtcgac accggcctgt tcgactggtc ggcctacgcg aacatcctgc cgccctacaa
     1861 gaagggcccg cccgacaagt ggggcctgct gcaggtcagg aagaagggcc tgcgcgacat
     1921 gggcggcacg ctgcgagccg aggacgcgca ccgcatcgcc gtcggcgccg agacgctgca
     1981 gctgcgcatg cggcgcgtct gcgacaacgc gctcgcgctg gcgcggtggc tggagcagca
     2041 gcccgcgatc gcccgcgtgt actacccggg cctggcgagc cacgcgcagc acgaacgcgc
     2101 ggcgcgcctg ttcaagggcg acttcggcct tctcatgagc ttcgagctgc gcgagggcat
     2161 cgacaccctt gcgttcctcg acgcgctgca gctggtcatc gtctccagcc acctggccga
     2221 caaccgcacg ctcgcgatcc cggtcgccca gacgatctac cacgagatgg gcccgcagcg
     2281 ccgcgcggag atgggcatcg ccgacggcct gatccggctg tcggtgggca tcgaggacac
     2341 cgccgacctg atcgacgatt tcacgcgcgc gctgcaggcg gtcacgcgct gagcgttccg
     2401 gccggtcggg gtcagccggt cggggtcagg tcttgaatcg ttgcgcgctc aaccagccgg
     2461 tcggggtcag gtcttgaatc gttgcgcgct caaccggttc ccttgagata cggcgtcgcc
     2521 gcgcaacgat tcaagacctg accccgagac cgaccccgag acctgacccg agaacttatg
     2581 ctgcgctgcg ctccagcggc gccaccagcc gcaggcgcgc cacatcgcgg cccggcggcg
     2641 cgccgaacat tcggctgtac tcgcgactga actgcgaggc actgtcgtag cccacggcgt
     2701 agccgacggc ggccacgtcg cccgaatcgg ccatcagccg cgcgcgcgcc tcctgcagcc
     2761 ggatctgctt ctggtactgg atcggcgtca tcgcggtcac ggcacggaag tggcggtgga
     2821 aggacgactc gctcatcgtc gcgacctgcg ccagctgctc gacgctcagc ggctcggcga
     2881 agttctggcg gatgtggcgg atggcgtggc tcacctgcgc gaggcggctg tccgccaacc
     2941 cgaactggcg cacgcgtccg ccctgcgggc cgcgcagcag gcgccacagg atctcgcgct
     3001 cgatcaaggg acgcagcacg ggcgcgtcgc ggggggcgtc gagcagccgc agcaggcgca
     3061 ccatcgcctc gaccagttcg acgggcgccg catgcacgcc catgccgcag ggcgtggcga
     3121 ccagctcgtc ggcgccgggc gtctccagca gcagcgacgc gatcgtggcc gggttcagcg
     3181 acagcccgca ggcgaggtac ggttcatccg ccgacgcgcg cacgatgcgg ctggcgatcg
     3241 gcaggtcgac cgacgtgacg acgaagtcgc ccgcgccgta ctcgaacacg cggtcgccga
     3301 cgatggtgcg cttggcgccc tggacgacca gcccgaggct gggttcggtc acgcagtgga
     3361 tcgcctcggt cggcacgcgg gagatcgtcg cacgcacgcc cgcgagcgcg gtggcggggg
     3421 cgcccgggcg cgcgtgacgc gcgaccaggg cgttcagttc ggacagcggt tcggccatgg
     3481 gtgcattgga acgcggcggc aaagccagca caacgggcgg gtgcatggaa gccgcgcgat
     3541 tggcaggatc gtgcaatcaa ttgcgagccc gctgctaccg ccgcgacggg cccggggcgc
     3601 agcatggagg ccttcgcaaa cgaaaggaat ctcccatgag caagacctgg ttcatcaccg
     3661 gcgccacccg cggcctgggc gccgacatcg ccgccgccgc gctgaaggcg ggcgaccgcg
     3721 tcgtcgccac cggtcgccag cgcgcggccg tcagcgaccg cctggggcct gactccgacc
     3781 gcctgctgtc gctgtcgctc gacgtcaacg acccggcgca ggcgcaggcg gccgccgcgg
     3841 cggcggtcga gcgcttcggc gcgatcgacg tgctggtcaa caacgccggc tacgggcaca
     3901 tcggcttctt cgaggaatcg tcgccggccg acatcgaggc gcagttcgcc accaacgtgt
     3961 tcggcctgct caacgtgacg cgcgcggtgc tgccggcgat gcgcgcggcg cgccgcggcc
     4021 acgtgttcaa cctgtcgtcg accgccggca tccgcggcat cgcggccggc tcgctgtact
     4081 gcgcgaccaa gttcgcggtc gagggcttct cggagtcgct cgcccaggag ctggcgccgt
     4141 tcggcatcca cgtgaccatc gtcgagccgg gcccgttccg caccgacttc ctgacgcccg
     4201 agtcgctgaa gttcacggct gccgaggtgg cggactacga cgcgcgccgc gcggagatgc
     4261 gcgccagctt cgagcaacgc aacggcaagc aggcgggcga tccggtgcgg ctggcgcagg
     4321 cgatcgtgac gctgtccaaa gaggcggcgc cgccgatgcg gttcgcggcg ggcgcgatgg
     4381 cggtcagcgc gttggatgcc aaggtcgcat cgctgaaggc ggagctcgat cgctggcgcg
     4441 agctggccat cggcaccgac tatccggccg ggacgtgacg cgaacggcgg gtgacgcctg
     4501 cggctgaccc gccctacgaa gcgcaggatt cggcgggcgg cggcgaccgt agggcgggtt
     4561 agccgaaggc gtaacccgcc atcgcccccg ggccccggat caatcggcca gcaactgccg
     4621 cagcacgtac ggcaggatgc cgccgtgcgc gtagtagtcc
//
LOCUS       DATCRO010000447         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_5287, whole genome shotgun sequence.
ACCESSION   DATCRO010000447 DATCRO010000000
VERSION     DATCRO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5287"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VIN75_12110"
     CDS             <1..1480
                     /locus_tag="VIN75_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974958.1"
                     /translation="QRFESSSREGEATIAVIARSARYGWTVAVGLPRSALDKPAIRVT
                     LQAFAAAGILAVIGLVLALSDAAAELGRDRVPAPLRTGLFEADQVGAALHEAGLRSAR
                     ATEVLESRVAEAIRQTQEAQTRLLDGQKHEAIGRLTGGLAHDLNNFLQTMSTGLQVLD
                     RLVHEETGRRVIEASTRALDKAATLVRQMLAFGRTQPLKPQPIDLHDFVLRCEDLMGR
                     AVGGKVRLTADIEPGLPPLMADPTQFELALLNLVFNARDAMPEGGTVMIRGRLASPTQ
                     ASALPPGRYLAVDVVDTGGGMDAEVLQRVFEPYFTTKPVGVGSGLGLPQVRAFAVQSG
                     GIATISSERGVGTTVSLLLPVSSDPVLAGVAAAAASAEFMPLRVLFVEDDLLVSSVVV
                     PALRAAGHAVRHCHTGDAAARALQDGGLFDVVFTDVVMPGTMSGLDLAAWCRANVPGI
                     AVIVATGYTTQHIDAGIKVLSKPYSIEALLKELQYAVRVKRA"
     gene            1529..2599
                     /locus_tag="VIN75_12115"
     CDS             1529..2599
                     /locus_tag="VIN75_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY8974959.1"
                     /translation="MTDVRKGQAPGQISRTEFGARFQSSFRDPAFQAEKDALGRLEEI
                     AWVAYKAGRKAPVTRKAGPGFADPDYDLSVDWLAARDALAAAQARQADPASPSRVLMI
                     LGASRNDGSCPGEISKTFRLSRWMAEELEGQGIEVDTLDLSLTTSEYGRSIHPCKGCV
                     STAMPLCHWPCSCYPNHSLRQTGDWMNEIYPRWAGAHGVIIATPVYWYQSPSPLKLMI
                     DRLVCADGGNPDPTSTHGKKAEEAKAIELAGWDYPQHLAGRVYGLMVHGDVAGIDSTR
                     RSLSDWLDWMGLIDAGPQALLDRYIGYWKPYATSHEDLDLDLAVQQEARNVALAVAHG
                     VKELRTGTLAAVQPPLRRPRPK"
     gene            complement(2666..3112)
                     /locus_tag="VIN75_12120"
     CDS             complement(2666..3112)
                     /locus_tag="VIN75_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY8974960.1"
                     /translation="MERATRQNTAIREALAAAGRPLSPAEVLDEARRHVAALGLATVY
                     RNLKALLDAGEIQVVTLPGEAPRYEPARREHHHHFRCDVCQRVFDVHQCPGDLAGLAP
                     PGFSVSRHEITLYGRCSDCQPAPQGRAKKKTPAAGHAKAHPHGHSH"
     gene            3164..>3629
                     /locus_tag="VIN75_12125"
     CDS             3164..>3629
                     /locus_tag="VIN75_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974961.1"
                     /translation="MFSGISPVVLAGAAVVSTLGGGALALRFRDRLHYLLGFTAGVLL
                     GVVAFDLLPEIFSLSREHAFDPSGAMIALVVGFLLFHSLEKFVLVHPAQEEHYSHHHH
                     PHVGLASAAVLAAHSCMDGVAIGIGWQVSPMVGLTVALAVIAHDFSDGLNTVT"
BASE COUNT          497 a         1277 c         1322 g          533 t
ORIGIN      
        1 ccagaggttc gaatcgagct cgcgcgaggg cgaggccacc atcgccgtca tcgcccgctc
       61 ggcgcgctac ggctggacgg tggccgtggg cctgccgcgc tcggccctgg acaagccggc
      121 gatccgcgtg acgctgcagg cgttcgccgc ggccggcatc ctggcggtga tcgggctcgt
      181 gctcgcgctg agcgacgcgg cggcggaact gggacgcgac cgcgtgcccg cgcccttgcg
      241 cacggggctg ttcgaggccg accaggtggg cgccgcgctg cacgaggcgg gcctgcgttc
      301 ggcccgcgcg accgaggtgc tggaatcgcg cgtggccgag gcgatccgcc agacgcagga
      361 ggcgcagacc cgcctgctgg acggccagaa gcacgaggcc atcggccggc tcacgggcgg
      421 cctcgcgcac gacctgaaca actttctgca gacgatgagc accggcctgc aggtgctcga
      481 ccgcctcgtg cacgaggaga cgggccggcg cgtgatcgag gccagcaccc gcgcgctgga
      541 caaggccgcc acgctggtgc ggcagatgct ggccttcggc cgcacgcaac cgctcaagcc
      601 gcagccgatc gacctgcacg acttcgtgct gcgctgcgag gacctcatgg gccgcgcggt
      661 gggcggcaag gtgcggctga cggccgacat cgagcctggc ctgccgcccc tcatggccga
      721 cccgacgcag ttcgagctgg cgctgctgaa cctcgtgttc aacgcgcgcg acgcgatgcc
      781 cgagggcggc accgtgatga tccgtggccg gctcgcgagc ccgacgcagg cgtccgcgct
      841 gccgcctggg cgctacctcg ccgtcgacgt cgtcgacacg ggcggcggca tggacgccga
      901 ggtgctgcag cgcgtgttcg agccgtactt caccaccaag ccggtgggcg tgggcagcgg
      961 cctggggctg ccgcaggtgc gcgccttcgc cgtgcagtcg ggcggcatcg ccacgatcag
     1021 cagcgagcgc ggcgtgggca cgacggtgtc gctgctgctg ccggtgtcca gcgatccggt
     1081 gctggccggt gtcgccgccg cggcggcgtc ggccgaattc atgccgctgc gcgtgctgtt
     1141 cgtcgaggac gacctgctcg tatcgtccgt ggtggtgccg gcgctgcgcg cggccggaca
     1201 cgcggtgcgc cactgccaca ccggcgacgc cgcagcacgc gcattgcagg acggcgggct
     1261 gttcgacgtg gtgttcacgg acgtcgtgat gcccggcacg atgagcggcc tggacctggc
     1321 ggcctggtgc cgggccaacg tgcccgggat cgccgtcatc gtggccaccg gctacacgac
     1381 gcagcacatc gacgccggca tcaaggtgct gtccaagccg tactcgatcg aggcgctgct
     1441 caaggaactg cagtacgcgg tgcgcgtcaa gcgcgcgtag gcgctccgtt tgccgacgct
     1501 gtcgcatcgg gcagacggga aggcagccat gaccgacgtt cgcaagggcc aggccccggg
     1561 ccagatctcg cgcaccgagt tcggcgcgcg cttccagtcc tcgttccgcg atccggcgtt
     1621 ccaggccgag aaggacgcgc tgggccgtct cgaggagatc gcctgggtcg cctacaaggc
     1681 cgggcgcaag gcgccggtga cgcgcaaggc cggccccggc ttcgcggatc cggactacga
     1741 cctgtcggtg gactggctcg ccgcgcgcga cgcgctcgcc gcagcgcaag ccaggcaggc
     1801 cgacccggcg tcgccgtcgc gcgtgctcat gatcctgggt gcctcgcgca acgacggcag
     1861 ctgcccgggc gagatctcca agacgttccg gctgtcgcgc tggatggccg aggaactcga
     1921 ggggcagggc atcgaggtcg acacgctcga cctgagcctg acgacgtccg agtacggccg
     1981 cagcatccac ccgtgcaagg gctgcgtgtc caccgcgatg cccctgtgcc actggccctg
     2041 cagctgctac ccgaaccact cgctgcgcca gaccggcgac tggatgaacg agatctatcc
     2101 gcgctgggcg ggcgcgcacg gcgtcatcat cgccacgccc gtctactggt accagtcgcc
     2161 cagcccgctc aagctgatga tcgaccgcct cgtgtgcgcg gacggcggca accccgaccc
     2221 gacgtcgacg cacggcaaga aggccgagga ggccaaggcc atcgagctgg ccggctggga
     2281 ctacccgcag cacctggcgg gccgggtcta cgggctgatg gtgcacggcg acgtcgccgg
     2341 catcgacagc acgcgccgca gcctcagcga ctggctggac tggatgggcc tgatcgacgc
     2401 cgggccgcag gcgctgctgg atcgctacat cggctactgg aagccgtatg cgacgagcca
     2461 cgaggatctc gacctcgacc tcgccgtgca gcaggaggcg cgcaacgtgg cgctggcggt
     2521 ggcgcatggc gtcaaggagc tgcgtaccgg cacgctggcc gcggtgcagc cgcctctgcg
     2581 ccggccgcgg cccaagtgag gcgaaaaggc cgtcatcctg cgcgcagtcg caggatccac
     2641 gttgacgggc cccggcggtc gccggctagt gcgagtgccc gtgcgggtgc gccttcgcgt
     2701 gcccggcggc cggcgtcttc ttcttcgcgc ggccctgggg cgccggctgg cagtcgctgc
     2761 agcggccgta cagcgtgatc tcgtggcgcg agacgctgaa gcccggcggc gcgaggccgg
     2821 ccaggtcgcc cgggcactgg tgcacgtcga acacgcgctg gcagacgtcg caacggaaat
     2881 ggtggtgatg ctcgcgacgc gccggttcgt agcgcggcgc ctcgccgggc agcgtgacga
     2941 cctggatctc gccggcgtcc aacagcgcct tgaggttgcg gtacaccgtg gccaggccca
     3001 gcgccgcgac atgccgccgt gcctcgtcca gcacctcggc gggcgagagc gggcgtccgg
     3061 cggcggctag cgcctcgcgg atggcggtgt tctggcgggt ggctcgttcc acgccgaaat
     3121 tctgccacgc ctgctgatat tgagtatcca ttatcattaa gcaatgttct caggcatctc
     3181 ccccgtcgtg ctcgccggcg ccgccgtcgt ctcgacgctg ggcggcgggg cgctcgcgct
     3241 gcgcttccgc gaccggctgc actacctgct gggcttcacc gcgggcgtgc tgctgggcgt
     3301 ggtcgcgttc gacctgctgc ccgagatctt ctcgctgtcg cgcgagcacg cgttcgatcc
     3361 gtcgggcgcg atgatcgcgc tggtggtcgg cttcctgctg ttccacagcc tcgagaaatt
     3421 cgtgctcgtc caccctgcgc aggaagagca ctactcgcac catcatcatc cgcatgtcgg
     3481 gctggcctcg gcggcggtgc tcgccgcgca cagctgcatg gacggcgtgg cgatcggcat
     3541 cggctggcag gtctcgccga tggtgggcct gacggtggcg ctggcggtga tcgcgcacga
     3601 cttctcggac ggcctgaaca ccgtcacgc
//
LOCUS       DATCRO010000448         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_859951, whole genome shotgun sequence.
ACCESSION   DATCRO010000448 DATCRO010000000
VERSION     DATCRO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859951"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VIN75_12130"
     CDS             <1..635
                     /locus_tag="VIN75_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin"
                     /protein_id="HEY8974962.1"
                     /translation="TWTATNLPALSVIGGWPRSYRLVADRRNAAKVYAYDSGGAWWGS
                     PGKVYVSTDGGHTFALSQGSVAANLRANPFNATSMAVNPNVEGDLWLADGNTVYHSVD
                     SGATWSKLNNFASIWGGRDPSQWPDVQGASVVALGKAKAGATYPAAVYVIGVINGVSG
                     VYRSDDGGTTWVRHNDDAHQYAGLGVMAADQGVYGRVYAGGSCRGVVYSN"
     gene            962..3613
                     /locus_tag="VIN75_12135"
     CDS             962..3613
                     /locus_tag="VIN75_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY8974963.1"
                     /translation="MLQSNIAGKGRARADGPCRSAGPGRTIRRAVAWAVCLASIAGAH
                     AAPSVRASAQEQAAALVARMTLDEKLGQLVNVAPPIERLGIPAYNWWTESLHGAIGPL
                     PTTNFPEPIGLAATFDAPLVHQVAATIGSEVRALHALGPGGVGLDTWAPNINIFRDPR
                     WGRGQETYGEDPWLTARLGVAFVTGMQGSDPDRPDVVATPKHFAVHSGPESTRHSANV
                     VVSPHDLEDTYLPAFRAAIVEARAGSVMCAYNRVNGQPACASEPLLEHRLRGAWGFDG
                     YVVSDCDAVKDIAEGHKYAPDAAAAVAAAMKAGVDNECNTSAWSGAAALRQRYADARD
                     RGLIGDADIDRALVRLFSARLRTGDLGGLAPRAPVPATEIGAPSHRALAFKSAIESLV
                     LLKNDGVLPLRSGVRIAVIGPHGDSTRVLRGNYSSERTIAAVSLLDGLRQAMPDSPVV
                     HVPFTASITDGDPVPTSALRTPDGQPGLRADYFNGADGGGFAANPVDTRIEPQVVSNA
                     ALAHGVDGRHKVVWTGFLVPPESGTYRIAMTGVKGTLATDGTPVRAADYSGWNEPLKF
                     TDVVLAKGRPYALRLEAESGGVAPGLHWKRVSTDLDAEVRAAVARSDVVVAAVGLTSD
                     IEGEEMPLSIDGFAGGDRTTLDLPADQRRLLESAQALGKPLVVVLMSGSAVDLSWAKS
                     HAAAIVEAWYPGEAGGLAIADVLAGHADPGGRLPVTFYRSAADLPPFDDYSMKGRTYR
                     YFGGTPVYPFGFGLSYARFGYSALHVRPVGGRPEQGLRVSVMVRNTSARAGDDVVQLY
                     ITPPALEGAPRHALRGVRHISLKAGEARALTFDLSPRDLSFVDAAGTRSVAPGRYRLS
                     VGAGQPGGDAATISTTYRVQAEVRLPE"
     gene            3756..>5272
                     /locus_tag="VIN75_12140"
     CDS             3756..>5272
                     /locus_tag="VIN75_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY8974964.1"
                     /translation="MRTTLRLLTATILAALFTAGSACAGTRERLSLDAGWLFHLGDVV
                     PPVAKGHGMSYGGAKAGNAGGAAATDYDDSGWRRLDLPHDWAVEGPFDPSENASQGYR
                     PRGIGWYRRYFNAPPSDRGRHIELQLDAVATHSQVWVNGIVVNRNWSGYNGRNIDITP
                     YLRYGEDVNVIAVRVDADAQEGWWYEGAGIYRHTWLVKRDAVHIATDGVHADPVRKGE
                     RWTLPVTVDVESSAKAAHRAELEVTVLDPAGRQVARGRTPVQVGALDASTARLELTVS
                     KPVLWTLQRPALYRVRSVLRDAGGELDRADVQTGFRTERFDADRGFFLNGVPTKLQGV
                     AIHQDHAGVGVAVPESIWAFRLRQLKAMGVNAVRFAHNAPAAEVLDLADRLGLLVMDE
                     NRNFNPSPDTMQQLTWLVRRDRNHPSVILWSIFNEEPLQGSEVGYEMARRMVAAVRKL
                     DTTRPVTAAMHDGLFGDVNVTQAVDVVGLNYQTWAYDRFHRAHPTQPMTSTEDTSA"
BASE COUNT          795 a         1887 c         1858 g          732 t
ORIGIN      
        1 caacctggac cgcgaccaac ctgccggcgc tcagcgtcat cggcgggtgg ccgcgcagct
       61 accgcctggt cgccgatcgc aggaacgcgg ccaaggtcta tgcatacgac tcgggcggtg
      121 cgtggtgggg cagcccgggc aaggtgtacg tctcgaccga tggcggccac accttcgcgc
      181 tgagccaggg ctcggtggcg gccaacctgc gcgccaatcc cttcaacgcc acctcgatgg
      241 cggtgaaccc gaacgtcgag ggcgacctgt ggctggccga cggcaacacc gtgtaccact
      301 cggtcgactc gggcgccacg tggtcgaaac tcaacaactt cgcctcgatc tggggtggcc
      361 gcgatccctc gcaatggccc gacgtgcagg gcgccagcgt cgtcgcgctg ggcaaggcca
      421 aggccggcgc gacgtacccg gcggcggtgt acgtgatcgg cgtgatcaac ggcgtgtcgg
      481 gcgtctaccg ctcggacgac ggtgggacga cctgggtgcg ccataacgac gacgcccacc
      541 agtacgccgg actgggcgtc atggcggctg accaaggcgt ctacggccgg gtgtacgcgg
      601 gcggcagctg ccgaggcgtg gtgtacagca actgaaggcc ggccacggag gccagggcgg
      661 cagcatccgc gccgccgccc tggcccgtgc aggacgccgc ccggcacacc ggcgacgagg
      721 cccccggtta gcgtcccgtt atgactccgg accccgcgcg gcttagcctg cgcacccggc
      781 gtcccgcgcc tcaaggtgca tgaagaaggc accatcgaac atgcatgccg ctcgtttcca
      841 tgaagcagat ccttcatccg accgcaccga gtccgtcgcg ccggcgatcc tcgacgagtc
      901 ctggtccgtg gaccggtggc tgccccgtca cctgacgggc ggcgcccgct gattccgaac
      961 catgctgcaa tcgaacattg ccgggaaagg gcgcgcccgc gccgacggcc cgtgccgcag
     1021 cgccggtccg ggccgcacga tccgccgggc cgtcgcctgg gccgtctgcc tggcctcgat
     1081 cgccggggcc cacgccgcgc catcggtgcg cgcctccgcg caggagcagg ccgccgcgct
     1141 ggtggcccgc atgacgctgg acgagaagct ggggcagctc gtcaacgtgg cgccgcccat
     1201 cgagcggctg ggaatcccgg cctacaactg gtggacggaa tcgctccatg gcgcgatcgg
     1261 gccgctgccc acgaccaatt tccccgagcc gatcgggctg gcggcgacgt tcgacgcgcc
     1321 cttggtccac caggtcgccg cgaccatcgg ctcggaagtg cgcgcgctgc acgcgctcgg
     1381 gcccggcggc gtcggactgg atacctgggc gcccaacatc aacatcttcc gcgacccgcg
     1441 ctggggccgg ggccaggaga cctacggcga ggatccctgg ctcaccgcgc gcctgggcgt
     1501 cgccttcgtc acggggatgc agggcagcga ccccgatcgt ccggacgtgg tcgccacgcc
     1561 caagcatttc gcggtgcaca gcggacccga atcgacgcgg cacagcgcga acgtcgtcgt
     1621 gtcgccgcat gacctggaag acacctacct gcccgcgttc cgcgcggcca tcgtcgaggc
     1681 cagggccgga tcggtcatgt gcgcctacaa ccgcgtcaat ggccagcccg cgtgcgcgag
     1741 cgagccgctg ctcgagcacc ggctgcgcgg cgcctgggga ttcgacggct acgtggtctc
     1801 cgactgcgac gccgtcaagg acatcgccga aggccacaag tacgcgccgg atgccgccgc
     1861 cgccgtggcc gccgcgatga aggcgggtgt cgacaacgag tgcaacacgt cggcgtggag
     1921 cggcgcggcg gccctgcgcc aacgctacgc ggatgcgcgc gatcgcggct tgatcggcga
     1981 tgccgacatc gaccgcgccc tcgtccgcct gttctccgcc cgcctgcgca cgggcgacct
     2041 gggcggcctg gcgcccaggg cgccggtgcc cgcgacggag atcggcgcgc cgtcgcatcg
     2101 cgccttggcg ttcaagagcg ccatcgaaag cctggtgctg ctgaagaacg acggcgtcct
     2161 gcccctgcgg tccggcgtcc gcatcgccgt gatcggaccg cacggcgact ccacgcgcgt
     2221 gctgcgcggc aactattcct ccgaacgcac catcgctgcg gtgtcgctgc tggacgggct
     2281 tcgacaggcg atgccggact ctccggtggt ccacgttccc ttcacggcct cgatcacgga
     2341 cggcgacccg gtgcccacgt cggccttgcg gacgccggac ggccagcccg gcctgcgggc
     2401 cgactacttc aacggggcgg atggcggtgg cttcgcggcc aatccagtgg acacccgcat
     2461 cgagccgcag gtcgtgtcga acgcggcgct cgcgcatggc gtggacgggc gtcacaaggt
     2521 cgtctggacg ggtttcctgg tgcccccgga gagcggcacg tatcgcatcg ccatgacggg
     2581 cgtcaaggga acgctggcca ccgatggcac gcccgtgcgc gcggccgact attccggctg
     2641 gaacgagccg ctgaagttca ccgacgtcgt gctggccaag ggccgcccct atgcgctgcg
     2701 gctcgaagcc gaatccggcg gcgtggcccc cggcctgcat tggaagcgcg tcagcaccga
     2761 cctcgacgcg gaggtgcggg ccgccgtcgc ccggtccgac gtggtcgtgg ccgcggtcgg
     2821 cctgacctcg gacatcgaag gcgaagagat gccgctgtcg atcgacggct tcgcgggcgg
     2881 ggatcgaacc acgctggact tgccggccga ccagcgcagg ctgctcgaaa gcgcacaggc
     2941 gctcggcaag cccctggtcg tcgtgctgat gagcggcagc gcggtcgact tgagctgggc
     3001 caagtcgcat gccgcggcca tcgtcgaggc gtggtatccc ggcgaggccg gaggcctggc
     3061 gattgccgac gtgctggccg gccacgccga tcccggaggg cgcctgccgg tcaccttcta
     3121 tcgcagcgcc gcggatctgc cgccattcga cgactattcg atgaaaggcc gcacctaccg
     3181 ctacttcggc ggtacgccgg tctacccgtt cggattcggg ttgagctacg cgcgattcgg
     3241 ctacagcgcg ttgcacgtcc ggcccgtggg gggccggccg gagcagggcc tgcgcgtctc
     3301 ggtcatggtc cgcaacacca gtgcgagggc gggcgacgac gtggtccagc tctacatcac
     3361 gccacccgcg ctcgaggggg cgcccaggca tgccttgcgc ggcgtccggc acatcagcct
     3421 gaaggccggc gaggcgaggg cgctgacgtt cgacctgagc ccccgggacc tgagcttcgt
     3481 cgatgcagcc ggcacgcgca gcgtggcccc ggggcgctat cggctgtcgg ttggcgccgg
     3541 ccagccgggc ggtgacgctg cgacgatatc cacgacctac cgtgtgcaag ccgaggtgcg
     3601 actgccagag tagccgtcgc gccacaggcc gctgccccgc aaggtgtgtc ggcttgcggc
     3661 gcatcctcga cgcagcctgg cggctgcggg caagtggcgg ttgccggaag cgcgaactcc
     3721 gaatcactcg aggacgccac gaacgagacc cgaacatgcg tacgactctc cgactcctga
     3781 ccgcgaccat cctcgcggcg cttttcaccg cgggcagcgc ctgtgccggc acccgcgagc
     3841 gactgtcgct ggacgcgggc tggctgttcc acctgggcga tgtcgtcccg cccgtggcca
     3901 aggggcatgg catgagctac ggcggcgcca aggcgggcaa cgcgggcgga gccgccgcga
     3961 ccgactacga cgactccggc tggcgtcggc tcgacctgcc gcacgactgg gcggtggaag
     4021 ggccgttcga cccgagcgag aacgcctcgc aaggctatcg accgcgcggc atcgggtggt
     4081 accggcgcta cttcaacgcg ccgccgtcgg accgtgggcg ccacatcgaa ctgcagctcg
     4141 acgccgtcgc cacgcacagc caggtgtggg tcaacggcat cgtcgtcaac cgcaactggt
     4201 ccggctacaa cggccgcaac atcgacatca cgccctacct gcgctacggc gaggacgtca
     4261 acgtgatcgc ggtgcgcgtg gacgccgatg cgcaggaggg ctggtggtac gagggcgccg
     4321 gcatctatcg ccacacctgg ctcgtcaagc gcgatgcggt gcacatcgcc accgacggcg
     4381 tccatgccga cccggtgcgc aagggcgagc gctggaccct gccggtcacc gtggacgtgg
     4441 agagcagcgc gaaggcggcg caccgcgccg agctcgaggt gaccgtgctg gaccctgccg
     4501 gccgccaggt ggccaggggc cgcacgcccg tgcaggtggg cgcgctggac gcgtcgaccg
     4561 cccggctcga gctgaccgtg agcaagcccg tgctctggac cctgcaacgg cccgcgctgt
     4621 accgcgtgcg gtcggtgctg cgcgacgcgg gcggcgagct cgaccgggcc gacgtgcaga
     4681 caggctttcg caccgagcga ttcgacgccg accgcggctt cttcctcaat ggcgtgccga
     4741 ccaagctgca gggcgtggcc atccaccagg accacgccgg cgtcggcgtc gcggtccccg
     4801 agtcgatctg ggcgttccgg ctgcgccagc tcaaggcgat gggcgtgaac gccgtgcgct
     4861 tcgcgcacaa cgcgccggcc gccgaggtgc tcgatctcgc cgatcgtctc gggctgctgg
     4921 tgatggacga gaaccgcaac ttcaatccgt cgcccgacac catgcagcag ttgacctggc
     4981 tcgtgcggcg cgaccgcaac cacccgagcg tgatcctgtg gtcgatcttc aacgaggagc
     5041 cgctgcaggg cagtgaagtc ggctacgaga tggcgcggcg catggtggcg gcggtcagga
     5101 agctggacac cacgcgcccg gtcaccgcgg ccatgcacga cggtctcttc ggcgacgtga
     5161 acgtgaccca ggcggtcgac gtcgtgggcc tcaattacca gacctgggca tacgaccgct
     5221 tccaccgggc ccatccgacg cagcccatga ccagcaccga ggacacctcg gc
//
LOCUS       DATCRO010000449        16416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_466179, whole genome shotgun sequence.
ACCESSION   DATCRO010000449 DATCRO010000000
VERSION     DATCRO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466179"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VIN75_12145"
     CDS             <1..958
                     /locus_tag="VIN75_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8974965.1"
                     /translation="ASAMLYVPLKAPMRVRGVVALALRNPQRLAVPEQRRLLDTFASL
                     LAIALERIHYVDVAQTTTVQMESERLRNALLGAISHDLRTPLAALAGMADSLRLTQPP
                     LAPAHAEIAARLREAALRLNALVNNLLDMARLESGNVALNLQWQPLEEVVGSALRGLA
                     APSEGRHPIRVQLPADLPLLRFDAVLVERVLANLLENAAKYTPAGSPVTIGAAATRES
                     VEVWVADEGPGLPPGREALIFEKFERGRKESATPGVGLGLAICRAIVAAHGGTIRGET
                     RPEGGARFVFTLPRGEPPRIEDLDDGDDNAAMPRATDPENAR"
     gene            955..1653
                     /gene="kdpE"
                     /locus_tag="VIN75_12150"
     CDS             955..1653
                     /gene="kdpE"
                     /locus_tag="VIN75_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEY8974966.1"
                     /translation="MTAAERPAKVLVVEDEAEIRRFVRLSLQAEAMDVSEAENVARGL
                     IEAGTRRPDLVVLDLGLPDGDGLDFIRGLRAWSQIPVIVLSARTMEADKIAALDAGAD
                     DYLVKPFGTGELLARVRAQLRRHPRGSADTASVLEFGDVRIDLAARTVERAGTALHLT
                     PIEYRLLTHLASHPNRVLTHPQLLKAVWGPSHAEDTHYVRIYMGHLRAKLEPDPSQPQ
                     HLVTELGVGYRFVP"
     gene            1764..2396
                     /locus_tag="VIN75_12155"
     CDS             1764..2396
                     /locus_tag="VIN75_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974967.1"
                     /translation="MNAVRRLAFAMMAGAALGLAGCATHEAAAPVEHRYTETISSVLI
                     SEDGQRIAAIGDGHHYIFGAPEVLVKALRSPVHPQLTATFSTFHIDQRGVVSGDWLLT
                     LAPDASDAAREQARAVGLTPGPDGVYATAGIIKGQRFTGWTYKLRGAQDKLNKPYTIE
                     ITTEASAGDRAVDDAATPIRVAADGVQLIYWAPLAPVIVPILFLTRAKDH"
     gene            2407..4188
                     /locus_tag="VIN75_12160"
     CDS             2407..4188
                     /locus_tag="VIN75_12160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8974968.1"
                     /translation="MTPSEIDHQALTALRLLPDEPERAMRVWDDAFARATAAGLDRTL
                     LRLRIVKMHHQARFGDRHALGQKMLAATAEARAHGWRTESLLVDLLEVFLATLSANHA
                     DGMGDVEAIRAEAEAHLDPIELSWMELMAGHFRGYMLGWALEPERTYRALNRLIASPD
                     SPPGLVANVQMNIGHSHLLVGNVDLAATYLQQAWRGYDALPLTPRKLAAARAWAQCLM
                     AMNEYRRADAVMRQALDARAELPSTMFMAGALLVAAEARLKLGDPVGAAAMLEDAAGC
                     ADAETEPGVLVHLAYVRALLLAEQGDIPSAAEVGEAGCRAIPRNTHKLGVQACLDLTA
                     RLQAQVGNFQRAYELQCQVVAMRNDLAKKSAEVRHIDLHVDHQTHLARMEIEHARRER
                     TIAENAEAAIAQKNQLLEQRLREVERLQESLRELANRDALTGLYNRRYLGEALPGLVS
                     MAARRGDRIVVILIDLDHFKKINDVHGHQMGDLVLEGFADLVRDAFRAHDLCCRYGGE
                     EFCVVMADTDDIAAHERVEELLERLGERTFTSGDKELTRVGFSAGIVTFKADPRMDLD
                     LVFRRADQALYASKNAGRRQISALELA"
     gene            4380..4985
                     /locus_tag="VIN75_12165"
     CDS             4380..4985
                     /locus_tag="VIN75_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY8974969.1"
                     /translation="MLARLAPLAALSLAAAVQAANVEVLLSDAAGNPLADAVVMLEPV
                     GAHLPVKPLQGVQIVQHDLQFDPPVTVVTTGTAVMFPNQDTVKHHVYSYSPAKTFQIK
                     LYAGVPHTPIVFDKPGVAVLGCNIHDGMIAWVVVSDTPLWARSAAGGHARVADVPPGS
                     YQLHVWHASLAETTPPQLLPLTVGASDIDQRVRLGAGGLVK"
     gene            4982..7435
                     /locus_tag="VIN75_12170"
     CDS             4982..7435
                     /locus_tag="VIN75_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8974970.1"
                     /translation="MSAARHRWRGSLAARIALVFLVLLLAVQLASFAALRLSWTNHRR
                     GELPRRLETGSTLLQTLLDQRVDAAIDVGTAAARDNALRSTLLDPETDETTKRLTIVS
                     ALRSMKTRAKASDVAFIGMDGAVLYRTQPVISEDLVPIAATLRSKALVSSSARELMLV
                     DHKPYQMVLLPELAPVLRGWFLLAIPLNETFTGDMRRLSEFDVTVLARTGPRAPWTIA
                     STTLAPAFAADLAEQRWGTDAAAQAMVAVPTQGDELGVHATMVGGTGPGDTSFALLLS
                     ESIGDAVRLPDDLRMALIGITILAIVVFGAGSVVTARRVTTPLRGLASAAERLGEGDY
                     ATPMRGVAREDEVGALSQAFERMRVSIGENQEQVLRLAYWDGLTGLPNRARFRELVGD
                     AIRDAADRRLADGVESSVAVLMLDLDRFKHVNDVLGYAVGDKVLVAVAERLARALARD
                     GDVVARLSGDRFGVLLQRTDAQQAHAVALRIEQALENPLALGEHKVDMGAGVGIACWP
                     SHADTDDALLVRAEMAMYAAKQRSDGPVTYDPSIDATSAQTLTLISELRQAVDRNELR
                     LYLQPKLALEDRGVIGAEALVRWQHPTRGLVPPVQFIPFAEQTGFIRHLTMWVFEEAA
                     RHWQVLADEGLEIRLSVNLSTRDLLDLDLPHKFGALLARHAVPATAFCLEITESAIMD
                     DPQRALSTLDALSAMGFKLSIDDFGTGYSSLAYLKRLPVDELKIDQSFVRNMQADRDD
                     EMIVRSTIDLAHNLGITVVAEGVESGAVWNLLRELKCDQAQGYHMGRPMPVAEFSAWC
                     EGWGMRRLAADGEAVTLLH"
     gene            complement(7642..8403)
                     /locus_tag="VIN75_12175"
     CDS             complement(7642..8403)
                     /locus_tag="VIN75_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974971.1"
                     /translation="MPLPENIAATRARRACAGVLLALAAAKAPADPADYVFTPYTDIG
                     RWQLAYAVGSEHGRDGSRATQQVASIGGAPVARWYTSVYAAWAADDGGPFTLDEWTWV
                     NHLQLTTPGSGPVDVGALCELSRPHDRDEGRLGVTCGPTLQMDTDDLQLNLNLSLGKH
                     VGAAAPERPALGYQWQVKGLVAHGVEVGAQGFGSFGTWNDWAPASQQEHTLGPAVFLK
                     THAFGGPIRVDAAWLFGVGDGSPRHVVRMRVQHEF"
     gene            complement(8529..10406)
                     /locus_tag="VIN75_12180"
     CDS             complement(8529..10406)
                     /locus_tag="VIN75_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8974972.1"
                     /translation="MDTAHRRKALLPLTLGAIGVVYGDIGTSPLYTMSTVFGPEGGVP
                     LDTPHLIGAASTIFWALMMVVTLKYVLLILRADNRGEGGIMALTALASQASGGGARRP
                     ALLLLVGLFGAALFYGDSVITPAMSVLSAVEGMEVIEPGLKRFVLPIAIGVLIGLFVM
                     QRKGTDAVGKLFGPIITLWFVVLAVTGVHQIIQAPAVLDAFNPLYAIRFLVERGPLVF
                     AAVGAIVLALTGAEALYADMGHFGRRPIQLAWLGLVLPALTLNYMGQAALLLRDPKAL
                     DNPFFHLFPHAWLIPVVILATMATVIASQAVISGAFSMTRQAIQLGFLPRMQVVFTSE
                     HEAGQIYLPKVNWTLLVAVMAATIGFGSSAAMAAAYGIAVTVTMLITTGLTFFVVRHA
                     WKLPFAVAWGGTIVFFGLDALLVASCSTKFFQGGWFPIVMGLVIFTVMSTWKRGRELL
                     MESIRRDDPELLPFIESLVHHVEQRAPRTAVFMVGNPDTVPQALMHNLKHNCVLHEQN
                     LILSVVFHERAYVEDDEHVQVTRLADGFWRVCINYGFMDLPDIPASLAACAAHGIDLE
                     PMRTSYFISRETVVSTPGDGMWQWRERLYATMQRNAGSITEFFKLPNNAVIELGTRVQ
                     I"
     gene            10654..10995
                     /locus_tag="VIN75_12185"
     CDS             10654..10995
                     /locus_tag="VIN75_12185"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY8974973.1"
                     /translation="MLLTAQRAELNLADDEVARLTDACDSRLEVTQGFVWVTVEGDRN
                     DVVLGAGESFLIDSRDIVTVSAIRGAAALKIRANVGANRCRTPAERAGARPNRLQQVL
                     ASISLSSVAVA"
     gene            complement(11054..12466)
                     /locus_tag="VIN75_12190"
     CDS             complement(11054..12466)
                     /locus_tag="VIN75_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8974974.1"
                     /translation="MTAAALLYAVGAVLEGWLAAMLFLSGRKLRGPDEHWTLLLAGGL
                     ALNSVRSVLMTFGVGNVPLHLAANALTSSIAEAAVALITGALMHYVGLSPRARSRLWM
                     VAGPLILGIWAATTAGWVSRGGAFVVAIIIFLGWAVLFMHATVREPRSGHGLVVLATL
                     SFPAIVVGLKMGVLPLDMLPIAEIVPLNAIGIAVLTSGLVRAHQRALHEGERTAQALA
                     ARERAEADLRAVNESLEQRVAQRTGHLEETIAGLESFNRSVSHDLRGPLGGISGVARM
                     ARDKLGEGLLDEADAMMAVIARQADDSVAMVSALLELARAGKAEPQRGPVDTTALAGE
                     VVATLEAAGKTAKAEVVVQRLPAADADRELLRQVFANLVGNACKFAGAGQPARVEVGA
                     AATPEGQAYFVRDNGIGFAPDDAARLFRPFERLNGQRYEGFGVGLSIVQRIIDHHGGR
                     VWAEGMPGRGATFWFTLGRR"
     gene            complement(12607..13698)
                     /gene="ychF"
                     /locus_tag="VIN75_12195"
     CDS             complement(12607..13698)
                     /gene="ychF"
                     /locus_tag="VIN75_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY8974975.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDARLQVLSDIVSAQKIVPAIVEFVDIAGLVAGASKGEGLGNQFLSHIRETDAIVN
                     VVRCFEDDNVVHVNGKVDPISDIEVIQTELCLADLASVEKQLQRNVKVARAGDKEAAK
                     LVAVLEKCRAALDEARPVRSLDFTKEELPLVRSLFLITAKPAMFVGNVADNGFENNPF
                     LDRLREYAAAQKAPVVAICAKTEAELADMEAEDAAMFLAEMGQTEPGLNRLIRAAFTL
                     LGLQTYFTAGVKEVRAWTIPVGATAPQAAGVIHTDFEKGFIRAQTIAYDDYVAFKGEQ
                     GAKDAGKMRAEGKEYVVKDGDVLNFLFSS"
     gene            13814..14929
                     /locus_tag="VIN75_12200"
     CDS             13814..14929
                     /locus_tag="VIN75_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8974976.1"
                     /translation="MSKIDVRIHGSGIVGRALALKLARQGLGVGIDARPSAPAGGEQR
                     ADVRAYALNAASVELLRELKVWDGMAPDARTAVYDMRIAGDGQGALAFSAWQQTVPAL
                     AWIVDAAELEQVLETAIRFAPAVHAVDADAKAPLTVIAEGKAAAARDRLGVTFERHSY
                     GHSALAARVVSDVPHAGVARQWFRAPDVLALLPFDRPQPECSYGVVWSMPQAEAERLL
                     QSSDADFDDALDAATGGAAGKLHVRGPRASWPLMQARAAEVVGPGWALVGDAAHVIHP
                     LAGQGLNLGLADVVALAKVVAEREAWRALGDEALLRRYARARLWPTRAMAGAVDGLWQ
                     LFCAPAPGLRELRNHGMSLVDRLSPLKRFLVSRALGS"
     gene            14953..15729
                     /locus_tag="VIN75_12205"
     CDS             14953..15729
                     /locus_tag="VIN75_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY8974977.1"
                     /translation="MTKFARALRLLLAGSLALACLAAWSDDAAIRRSWNQRHPNGPAI
                     DEITRTPIAGLYELRIGADLVYSDEQGNYLIYPGRDAGNGQVPDGHIFDARKNVDITD
                     QRLDKLMEKEVPKLPYGDALVFKQGNGARKMVVFEDPNCHYCKDAERSFMQLKDVTIY
                     TFLIPILGDDSVAKARTIWCSKNNAQVWRDWMVNGVLPPRPMGACDMKVLDRNLELAA
                     RHHLNYTPAIIFEDGSRFAGNADLAHLTKRLDEVAAARKS"
     gene            15805..>16416
                     /locus_tag="VIN75_12210"
     CDS             15805..>16416
                     /locus_tag="VIN75_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEY8974978.1"
                     /translation="MIHYRIEVADAAAHQFKVTLTIADPAPVQQLSLPVWIPGSYMVR
                     EFSRHLSLLDARQGAQPRPIEQLAKDRWSVATSGRAALVVTYFVYAFDPSVRTAWLGD
                     DRGFFNTTSLCLAVDGRTAEPHRVELHRLPAGWDVATGMTRIDPGARTAGKAKGAKAV
                     PARTQVFESADYDEMADHPFELGPFWRGEFTSGGVVHEFVVAGA"
BASE COUNT         2452 a         5864 c         5772 g         2328 t
ORIGIN      
        1 ggccagcgcg atgctgtacg tgccgctgaa ggcgccgatg cgcgtgcgcg gcgtcgtcgc
       61 gctggcgctg cgcaacccgc agcggctcgc ggtgccggag cagcgccggc tgctcgacac
      121 cttcgcctcg ctgctggcga tcgcgctgga gcgcatccac tacgtcgacg tcgcgcagac
      181 gacgacggtg cagatggaat cggagcgcct gcgcaacgcg ctgctgggcg cgatctcgca
      241 cgacctgcgc acgccgctgg ccgcgctcgc gggcatggcc gattcgctgc ggctcacgca
      301 gccgccgctg gcgcccgcgc atgccgagat cgccgcgcgg ctgcgcgagg cagcgctgcg
      361 gctgaacgcg ctggtcaaca acctgctgga catggcgcgg ctggagtccg gcaacgtggc
      421 gctgaacctg cagtggcagc cgctcgagga ggtggtgggc agcgcgctgc gcgggctggc
      481 ggcgccgtcg gaagggcgtc atccgatccg cgtgcagctg ccggcggacc tgccgctgct
      541 gcgcttcgac gcggtgctgg tcgagcgcgt gctggccaac ctgctggaga atgcggccaa
      601 gtacacgccg gccggcagcc cggtcacgat cggggctgcc gccacgcgcg agtcggtcga
      661 ggtgtgggtg gccgacgagg gtccgggcct gccgccggga cgcgaggcgc tgatcttcga
      721 gaagttcgag cgcggccgca aggagagcgc cacgccgggt gtcggcctgg gcctggcgat
      781 ctgccgggcc atcgtggccg cgcacggcgg cacgatccgc ggcgagacgc gccccgaggg
      841 cggcgcgcgc ttcgtgttca cgctgccgcg tggagagccc ccgcgcatcg aggacctgga
      901 cgacggcgac gacaatgccg cgatgccccg cgccaccgat ccggagaacg cccgatgacc
      961 gccgccgagc gtccggccaa ggtcctggtg gtggaggacg aggccgagat ccgccgcttc
     1021 gtgcgcctgt cgctgcaggc cgaagccatg gacgtgtccg aggccgaaaa cgtcgcccgc
     1081 gggctgatcg aggccggcac gcggcggccc gacctcgtgg tgctggacct cgggctgccg
     1141 gacggcgacg gcctcgattt catccgcggc ctgcgcgcgt ggtcgcagat cccggtcatc
     1201 gtgctgtcgg cccggacgat ggaggccgac aagatcgccg cgctggacgc cggcgccgac
     1261 gactacctcg tcaagccctt cggcaccggc gagctgctcg cgcgcgtgcg cgcgcagctg
     1321 cgcaggcacc cgcgcgggag cgccgacacg gccagcgtgc tggagttcgg cgacgtgcgc
     1381 atcgacctcg cggcgcgcac cgtggagcgt gccggcacgg cgctgcacct cacccccatc
     1441 gaataccggc ttctgacgca cctggcctcg cacccgaatc gagtgctcac tcacccgcaa
     1501 ctgctcaagg ccgtgtgggg gccgtcccat gcggaggaca cgcactacgt gcgcatctac
     1561 atgggccacc tgcgcgccaa gctggagccg gacccctcgc agccgcagca cctggtgact
     1621 gaactgggag tcggctatcg gttcgttccc tgagaaatcg tttgcgcgga ccccgagttc
     1681 tcggtcgttt cctctagaga catccgtgtc aggccacgga taatcgcgcc tcccggtcca
     1741 ggaagccggg tggagcgacc ccggtgaatg ctgttcgacg tcttgccttc gcgatgatgg
     1801 ccggcgccgc cctcgggctg gccggctgcg ccacccacga ggcggccgcc ccggtggaac
     1861 accgctacac cgaaacgatc agctcggtgc tgatttccga ggacggccag cgcatcgcgg
     1921 ccatcggcga cggccatcac tacatcttcg gcgcgcccga ggtgctggtg aaggcactgc
     1981 gctcgccggt ccatccgcag ctgaccgcca cgttctccac cttccacatc gaccagcgcg
     2041 gcgtcgtcag cggcgactgg ctgctcacgc tcgcgccgga cgcctccgac gccgccaggg
     2101 agcaggcccg cgccgtcggc ctgacccccg gccctgacgg ggtctacgcg accgccggca
     2161 tcatcaaggg ccagcgcttc accggctgga cgtacaagct gcgcggcgcg caggacaagc
     2221 tcaacaagcc ctacacgatc gagatcacca ccgaggcgag cgccggcgac cgcgcggtcg
     2281 acgacgccgc cacgcccatc cgcgtggccg ccgacggcgt gcagctgatc tactgggcgc
     2341 cgctcgcgcc ggtgatcgtg ccgatcctgt tcctgacgcg cgccaaggat cactgaggtc
     2401 gtcgccgtga ccccgagcga gatcgaccac caggcgctca cggcgctgcg gctgctgccc
     2461 gacgaaccgg agcgcgcgat gcgcgtctgg gacgacgcgt tcgcgcgtgc gacggcggcc
     2521 gggctcgatc gcacgctgct gcggctgcgc atcgtcaaga tgcaccacca ggcgcgtttc
     2581 ggcgaccgcc atgcgctggg ccagaagatg ctggccgcca cggccgaggc gcgcgcgcac
     2641 ggctggcgca ccgaatcgct gctggtcgac ctgctcgagg tcttcctcgc gacgctcagc
     2701 gccaaccacg ccgacggcat gggagacgtc gaggcgatcc gggccgaggc cgaggcccat
     2761 ctcgacccga tcgagctgag ctggatggag ctgatggccg gccatttccg cggctacatg
     2821 ctgggctggg cgctcgagcc cgagcgcacc taccgcgcgc tcaaccgcct gatcgccagc
     2881 cccgattcgc cgccgggcct cgtggccaac gtccagatga acatcggcca cagccacctg
     2941 ctggtgggca acgtcgacct ggcggccacc tacctgcagc aggcctggcg cggctacgac
     3001 gcgctgccgc tgacgccgcg caagctggcg gccgcgcgcg cctgggcgca gtgcctgatg
     3061 gcgatgaacg aatatcgccg cgccgacgcg gtgatgcgcc aggcgctcga cgcccgcgcc
     3121 gagctgccgt cgacgatgtt catggccggc gcgctgctcg tcgccgccga ggcgcgcctg
     3181 aagctgggcg atccggtcgg cgccgccgcg atgctggagg acgccgccgg ctgcgccgat
     3241 gccgagacgg aacccggcgt gctggtgcac ctggcctacg tgcgcgcgct gctgctggcc
     3301 gagcagggcg acatcccgtc ggcggccgag gtcggcgagg cgggttgccg cgccatcccg
     3361 cgcaacacgc acaagctggg cgtccaggcc tgcctcgatc tcaccgcgcg gctgcaggcg
     3421 caggtcggca acttccagcg cgcctacgag ctgcagtgcc aggtggtggc gatgcgcaac
     3481 gacctggcca agaagtcggc cgaggtgcgc cacatcgacc tgcacgtcga ccaccagacg
     3541 cacctggcgc gcatggagat cgagcacgcg cggcgcgagc gcaccatcgc cgagaacgcc
     3601 gaggccgcga tcgcgcagaa gaaccagctg ctcgagcaac gcctccggga ggtcgagcgc
     3661 ctgcaggaat cgctgcgcga gctcgccaac cgcgacgcgc tgaccggcct gtacaaccgc
     3721 cgctacctcg gcgaggcgct gccggggctg gtgtcgatgg ccgcgcgccg cggcgaccgc
     3781 atcgtcgtca tcctgatcga cctggaccac ttcaagaaga tcaacgacgt ccatggccac
     3841 cagatgggcg acctcgtgct cgagggcttc gccgacctgg tgcgcgatgc gttccgcgcg
     3901 cacgacctgt gctgccgcta cggcggcgag gagttctgcg tggtgatggc cgacaccgac
     3961 gacatcgccg cgcacgagcg cgtcgaggag ctgctcgagc gcctgggcga acgcacgttc
     4021 accagcggcg acaaggagct cacgcgcgtg ggcttctcgg ccggcatcgt caccttcaag
     4081 gccgatccgc gcatggatct ggacctggtg ttccgccgcg ccgaccaggc gctgtacgcg
     4141 tcaaagaacg ccggccggcg ccagatctcc gcgctcgagc tggcgtgaca gcacgcaacc
     4201 cggcgcgtcg gggagggctt gctggacggc aagacggcgt gacggaatgc gcgcccggcg
     4261 cccggtcgtg acgcccgggt tcaggtgggg ccccccgcag ccgatatcct gatggcagtc
     4321 agcccggcgt cattcgacgc ggcggccgca tgcccatccg gaatccccgc ctcgcgccca
     4381 tgctcgcccg tctcgcgccc ctcgccgccc tgtcgcttgc cgccgccgtg caggccgcca
     4441 acgtcgaagt gctgctcagc gacgccgccg gcaacccgct cgccgacgcg gtcgtcatgc
     4501 tcgagcccgt gggcgcgcac ttgccggtca agccgctgca aggggttcag atcgtgcagc
     4561 acgacctgca gttcgacccg ccggtgacgg tggtcacgac cggcacggcg gtgatgttcc
     4621 cgaaccagga cacggtcaag caccacgtct actcgtactc gccggccaag accttccaga
     4681 tcaagctgta cgccggcgtg ccgcacacgc ccatcgtctt cgacaagccc ggcgtcgccg
     4741 tgctcggctg caacatccac gacggcatga tcgcgtgggt ggtcgtcagc gacacgccgc
     4801 tgtgggcgcg cagcgcggcc ggcggccacg ccagggtggc ggacgtgccg cccggcagct
     4861 accagttgca cgtgtggcac gcgtcgctgg ccgagaccac gccgccgcag ctgctgccgc
     4921 tgacggtcgg cgcatccgac atcgaccagc gcgtcaggct cggcgcgggc gggctcgtca
     4981 agtgagcgcg gcccggcacc gctggcgcgg ctcgctcgcc gcgcgcatcg cgctcgtgtt
     5041 cctcgtgctg ctgctggcgg tgcagctggc gagcttcgcg gcgctgcgcc tgagctggac
     5101 gaaccatcga cgcggcgagc tgccgaggcg gctcgagacc ggcagcacgc tgctgcagac
     5161 cctgctggat caacgcgtcg atgccgccat cgatgtcggc acggccgcgg ccagggacaa
     5221 cgcgctccgg agcacgctgc tggaccccga gaccgacgag acgaccaagc ggctcacgat
     5281 cgtctcggcg ctcaggtcga tgaagacccg ggccaaggcc agcgacgtcg cgttcatcgg
     5341 catggacggc gccgtgctct atcgcacgca gccggtcatc agcgaggatc tcgtgccgat
     5401 cgcggccacc ctgcggtcca aggccctggt ctcgagttcg gcgcgcgagc tgatgctggt
     5461 ggaccacaag ccgtaccaga tggtgctcct gccggagctg gcgcccgtgc tgcgcggctg
     5521 gttcctgctc gcgatcccgc tgaacgagac gttcacgggc gacatgaggc gcctgtcgga
     5581 attcgacgtg acggtgctgg cgcgcaccgg cccacgcgcg ccgtggacga tcgcctcgac
     5641 cacgctcgca ccggccttcg cggccgacct ggcggaacaa cgctggggca cggacgccgc
     5701 cgcgcaggcc atggtggcgg tgcccacgca gggcgacgag ctgggcgtgc acgccacgat
     5761 ggtgggcggc accggccccg gcgacacgag cttcgccttg ctgttgtccg agtccatcgg
     5821 cgacgccgtg cgcctgcccg acgacctgcg catggcgctg atcggcatca cgatcctggc
     5881 catcgtcgtg ttcggcgcgg gcagcgtggt gacggcgcgc cgcgtcacga cgccgctgcg
     5941 cggcctggcc agtgccgccg agcggctggg cgagggcgac tatgcgacgc cgatgcgcgg
     6001 cgtggcgcgc gaggacgagg tcggcgcgct gtcgcaggcg ttcgagcgca tgcgcgtgag
     6061 catcggcgag aaccaggagc aggtgctgcg cctggcgtac tgggacggcc tcaccggcct
     6121 gcccaaccgc gcgcgcttcc gcgagctggt gggcgacgcg atccgcgacg cggccgaccg
     6181 gcgcctggcc gacggcgtcg agtcgtcggt cgccgtgctg atgctcgacc tcgaccgctt
     6241 caagcacgtg aacgacgtgc tgggctacgc ggtgggcgac aaggtgctgg tcgcggtggc
     6301 cgaacgcctc gcgcgggcgc tggcgcgcga cggcgatgtc gtggcgcgcc tgagcggcga
     6361 ccgcttcggc gtgctgctgc agcgcaccga cgcgcagcag gcccatgcgg tggcgctgcg
     6421 catcgaacag gcgctggaga acccgctcgc gctcggcgag cacaaggtcg acatgggcgc
     6481 gggcgtgggc atcgcctgct ggccgtcgca tgccgacacc gacgacgcgc tgctggtgcg
     6541 cgccgagatg gccatgtacg ccgccaagca gcgcagcgac ggtcccgtca cctacgaccc
     6601 gtcgatcgac gccaccagcg cccagacgct gacgctgatc tccgagctgc gccaggcggt
     6661 cgatcgcaac gagctgcgcc tgtacctgca gcccaagctg gcgctggagg atcgcggcgt
     6721 gatcggcgcc gaggcgctgg tgcgctggca gcatccgacg cgcggcctgg tgccgcccgt
     6781 gcagttcatc ccgttcgccg agcagaccgg cttcatccgc cacctgacga tgtgggtgtt
     6841 cgaggaggcg gcgcgccatt ggcaggtgct ggccgacgaa gggctggaga tccgcctgtc
     6901 ggtcaacctg tccacgcgcg acctgctgga cctcgacctg ccgcacaagt tcggcgcgct
     6961 gctggcgcgc catgcggtgc cggccacggc cttctgcctc gagatcaccg agagcgcgat
     7021 catggacgac ccgcagcgcg cgctgtccac gctcgatgcg ctgagcgcga tgggattcaa
     7081 gctgtcgatc gacgacttcg gtaccggcta ctcgtcgctc gcctacctca agcggctgcc
     7141 ggtcgacgag ctcaagatcg accagtcctt cgtgcgcaac atgcaggccg accgcgacga
     7201 cgagatgatc gtgcgctcga ccatcgatct cgcgcacaac ctgggcatca ccgtggtggc
     7261 agagggcgtc gagagcggcg ccgtctggaa cctgctgcgc gagctcaagt gcgaccaggc
     7321 gcagggctac cacatgggcc gcccgatgcc ggtcgccgag ttctcggcct ggtgcgaggg
     7381 ctggggcatg cgccggctgg cggccgatgg cgaggcggtg acgctgttgc actgaggcac
     7441 ccggcccgag gggtctggcc tctcctcgac atcgcgccgg tctgccgccg tcagccactt
     7501 cgctcgtcgc gattcgagga caggcctgac cccgatcctt cgctggcaac cggggtcagg
     7561 cctgtccttc attcgcgcat cgtcctgacc ttgatcgcca acaacgcggg cgcgaattca
     7621 ggagaggcca gacccctcgg ctcagaactc gtgctgcacc cgcatccgca cgacatggcg
     7681 cggcgacccg tcgccaacgc cgaacagcca cgccgcatcc acgcggatcg gcccgccgaa
     7741 cgcgtgcgtc ttcaggaaca ccgccggtcc gagcgtgtgc tcctgctgcg acgccggcgc
     7801 ccagtcgttc cacgtcccga acgagccgaa gccctgcgcg cccacctcga ccccgtgcgc
     7861 caccaggccc ttgacctgcc actggtagcc cagcgccggc cgctcgggcg cggcggcgcc
     7921 gacgtgcttg ccgagcgaca ggttcaggtt gagctgcagg tcgtcggtgt ccatctgcag
     7981 cgtcgggccg caggtgacgc ccaggcggcc ctcgtcgcgg tcgtgcggac gcgagagctc
     8041 gcacagtgcg ccgacgtcga ccggtccgga gccgggcgtc gtcagctgca ggtggttgac
     8101 ccacgtccac tcgtcgaggg tgaacggccc gccgtcgtcc gcggcccacg cggcgtacac
     8161 cgacgtgtac cagcgcgcga cgggcgctcc gccgatgctc gccacctgct gcgtcgcacg
     8221 ggagccgtcg cggccatgct cgctgcccac ggcgtaggcg agctgccacc gtccgatgtc
     8281 ggtgtagggc gtgaagacgt agtcggccgg atcggccggg gccttcgccg cggcgagcgc
     8341 gagcagcacg cccgcgcaag cgcggcgcgc cctcgtggcc gcgatgttct ccgggagggg
     8401 catgcctggg ttatcggctc gggaatcgct cgattgaacc ccaggcgtgc ccgacgcggc
     8461 aatcccggga tgacgccgag tgcgacgcat gtctcggttt gcccgccggc gtgcggccgg
     8521 ccgcccgctc agatctgcac gcgcgtgccc agctcgatca ccgcgttgtt gggcagcttg
     8581 aagaactcgg tgatgctgcc ggcgttgcgc tgcatcgtcg cgtacaggcg ctcgcgccac
     8641 tgccacatgc cgtcgccggg cgtggagacc accgtctcgc ggctgatgaa ataactggtg
     8701 cgcatcggtt ccaggtcgat gccgtgcgcc gcgcaggccg ccagcgacgc cgggatgtcg
     8761 ggcaggtcca tgaatccgta gttgatgcac acccgccaga agccgtcggc caggcgcgtg
     8821 acctgcacgt gctcgtcgtc ctccacgtag gcgcgttcgt ggaacaccac cgacaggatc
     8881 aggttctgct cgtgcagcac gcagttgtgc ttgaggttgt gcatcagcgc ctgcggcacg
     8941 gtgtcgggat tgccgaccat gaagacggcc gtgcggggcg cgcgttgttc gacatgatgg
     9001 accagcgact cgatgaacgg cagcagctcc ggatcgtcgc ggcggatcga ttccatcagc
     9061 agctcgcggc cgcgcttcca tgtggacatc accgtgaaga tgaccaggcc catcacgatc
     9121 gggaaccagc cgccctggaa gaacttggtc gagcacgagg ccaccagcag ggcgtccagg
     9181 ccgaagaaga cgatggtgcc gccccaggcc acggcgaacg gcagcttcca ggcatggcgc
     9241 acgacgaaga aggtcaggcc ggtggtgatg agcatggtca ccgtcacggc gatgccgtac
     9301 gcggcggcca tcgcggccga gctgccgaag ccgatcgtgg cggccatcac ggccaccagc
     9361 agcgtccagt tgaccttggg caggtagatc tggccggcct cgtgctccga cgtgaagacg
     9421 acctgcatgc gcggcaggaa gcccagctgg atcgcctggc gcgtcatcga gaatgcgccc
     9481 gagatcaccg cctgcgacgc gatgacggtg gccatcgtcg ccaggatcac cacggggatc
     9541 agccaggcat gtggaaacag gtggaagaac gggttgtcca acgccttcgg atcgcgcagc
     9601 agcagcgccg cctggcccat gtagttcagc gtcagcgccg gcagcaccag gcccagccac
     9661 gcgagctgga tcggacgccg gccgaaatgg cccatgtcgg cgtacagcgc ctcggcgccc
     9721 gtcagcgcca gcacgatcgc gccgacggcc gcgaacacca gcgggccgcg ctcgaccagg
     9781 aagcggatcg cgtacaacgg gttgaatgca tcgaggaccg ccggcgcctg gatgatctga
     9841 tgcacgccgg tgacggccag gaccacgaac caaagcgtga tgatggggcc gaacagcttg
     9901 ccgaccgcgt cggtgccctt gcgctgcatc acgaacaggc cgatcagcac gccgatggcg
     9961 atcggcagca cgaagcgctt gaggccgggc tcgatgacct ccatgccctc gaccgcgctg
    10021 agcaccgaca tcgccggcgt gatgacgctg tcgccgtaga acagcgcggc gccgaacagg
    10081 cccaccagca gcagcagggc ggggcgccgg gcgcccccgc ccgaggcctg cgaggcgagc
    10141 gccgtcagcg ccatgatgcc gccctcgccg cggttgtccg cccgcaggat cagcagcacg
    10201 tacttgagcg tgaccaccat catcagcgcc cagaagatcg tcgaggcggc gccgatcagg
    10261 tgcggcgtgt cgagcggcac gccgccctcg gggccgaaga cggtcgacat cgtgtacagc
    10321 ggactggtgc cgatgtcgcc atagacgacg ccgatggcgc cgagggtcag gggcaggagg
    10381 gctttcctgc ggtgggcggt atccatggga gcgggagttg tcgcgagtcc ggcagtgtcg
    10441 ccgcgggcgc ataaaaaggg cataaagagg gtcgtcccag gggcggctag ccatgcgtcc
    10501 atggaatgag tgcgatgaca attgaacgtt ggatttgtct cagggaaagc ccgaagatac
    10561 gcctaccctg aagcgaatcg cagagacctc ccggaagacg gcgatggatg cgattgtgga
    10621 atagggtagc aagcctagaa ccggagaacg atcatgctgc tgactgccca acgcgccgaa
    10681 ctgaacctcg ccgacgacga ggtcgcccgc ctgaccgacg cctgcgactc gcgcctggaa
    10741 gtgacccagg gcttcgtctg ggtgactgtg gaaggcgatc gcaacgacgt cgtcctgggt
    10801 gccggcgaat ccttcctgat cgactcgcgc gacatcgtca ccgtcagcgc catccgcggc
    10861 gcggccgcgc tgaagatccg cgcgaacgtc ggcgcgaacc gctgccgcac gccggccgag
    10921 cgcgccggcg cacgtccgaa ccgcctgcag caggtgctgg cgagcatctc gctgagctcg
    10981 gtcgccgtcg cctgacgccc gcacgcggtc ggccggcata ccctgcggtg gacgccgctg
    11041 acggtcgacc gcgtcagcgc cggcccagcg tgaaccagaa cgtggcgccg cggccgggca
    11101 tgccctcggc ccacacgcgg ccgccatggt ggtcgatgat gcgctggacg atggacaggc
    11161 cgacgccgaa gccctcgtag cgctggccgt tcaggcgctc gaacgggcgg aacaggcgtg
    11221 cggcgtcgtc cggcgcgaag ccgatgccgt tgtcgcgcac gaagtaggcc tgcccctccg
    11281 gcgtggcggc ggcgcccacc tcgacgcgtg ccggctggcc ggcgcccgcg aacttgcagg
    11341 cgttgcccac caggttggcg aacacctggc gcagcagctc gcggtcggcg tcggccgccg
    11401 gcaggcgctg caccaccacc tcggccttgg ccgtcttgcc cgcggcctcc agcgtggcga
    11461 cgacctcgcc ggccagcgcc gtcgtgtcca ccggcccgcg ctgcggctcc gccttgccgg
    11521 cgcgcgccag ctccagcagc gccgacacca tcgccaccga gtcgtcggcc tggcgcgcga
    11581 tgaccgccat catggcgtcg gcctcgtcga gcaggccttc gccgagcttg tcgcgcgcca
    11641 tgcgggcgac ccccgagatg ccgcccagcg gcccgcgcag gtcgtgcgag acgctgcggt
    11701 tgaagctctc caggccggcg atggtctcct cgaggtggcc ggtgcgctgc gcgacgcgct
    11761 gctccagcga ctcgttgacg gcgcgcagat cggcctccgc gcgctcgcgc gccgcgagcg
    11821 cctgcgccgt gcgctcgccc tcgtgcagcg cgcgctggtg cgcgcgcacg aggccgctcg
    11881 tgagcaccgc gatgccgatg gcgttcagcg gcacgatctc ggcaatgggc agcatgtcca
    11941 agggcaagac tcccatcttc agtccgacca cgatggccgg gaagctgagc gtcgccagca
    12001 cgaccaggcc gtggcccgag cgtggctcgc gcaccgtggc gtgcatgaac agcacggccc
    12061 agcccaggaa gatgatgatc gccaccacga acgccccgcc gcggctgacc cagcccgccg
    12121 tcgtcgcggc ccagattccg aggatcaggg gcccggccac catccacagg cgactgcgcg
    12181 cgcgcggcga caggccgacg taatgcatca gcgcgcccgt gatgagcgcc acggcggcct
    12241 cggcgatgga gctggtgagc gcgttggcgg ccaggtgcag cggcacgttg cccacgccga
    12301 acgtcatcag cacggagcgc acgctgttca gggccaagcc gccggccagc agcagcgtcc
    12361 agtgttcgtc ggggccgcgc agcttgcgcc cgctcaggaa cagcatggcg gcgagccagc
    12421 cttcgagcac ggcaccgact gcatagagaa gggcggcggc ggtcatcgcg ccatgttgcg
    12481 ggaggactgc cgcgctggca atccgaggac acccctcgtc gtatcccggc ccccgatgga
    12541 aaggcccgcc ggttcgcggc gggcccgtcg tcgtgccggc gcgcgcatca cgcgcgcggg
    12601 cgaggttcag ctgctgaaga ggaagttcag cacgtcgccg tccttgacga cgtattcctt
    12661 gccttccgcg cgcatcttgc cggcgtcctt ggcgccctgc tcgcccttga aggcgacgta
    12721 gtcgtcgtag gcgatggtct gggcgcggat gaagcccttc tcgaagtcgg tgtggatcac
    12781 gccggcggcc tgcggggcgg tggcgccgac ggggatcgtc caggcgcgca cttccttgac
    12841 gcccgcggtg aagtaggtct gcaggcccag cagcgtgaac gcggcgcgga tcaggcggtt
    12901 caggcccggc tcggtctgcc ccatctcggc caggaacatc gcggcgtcct cggcctccat
    12961 gtcggccagc tcggcctcgg tcttggcgca gatcgcgacg accggggcct tctgcgccgc
    13021 ggcgtactcg cgcaggcgat ccaggaacgg gttgttctcg aagccgttgt cggccacgtt
    13081 gcccacgaac atcgccggct tggcggtgat caggaacagc gagcgcacca gcggcagctc
    13141 ttccttcgtg aaatcgaggc tgcgcacggg cctcgcctca tcgagcgcgg cgcggcactt
    13201 ctcgaggacg gccaccagct tggcggcttc cttgtcgccg gcgcgcgcca ccttgacgtt
    13261 gcgctgcagc tgcttctcga cgctggccag gtcggccagg cacagctcgg tctggatgac
    13321 ctcgatgtcg gagatcgggt cgaccttgcc gttgacgtgc accacgttgt cgtcctcgaa
    13381 gcagcgcacg acgttgacga tggcgtcggt ctcgcggatg tgcgacagga actggttgcc
    13441 caggccctcg cccttggacg cgcccgccac caggccggcg atgtcgacga actcgacgat
    13501 ggccggcacg atcttctgcg ccgagacgat gtccgacagc acctgcaggc gcgcgtcggg
    13561 cagctcgacg atgccgacgt tcggctcgat cgtgcagaac gggtagttct cggcggcgat
    13621 gccggccttg gtcagggcgt tgaacagggt ggacttgccg acattgggca ggccgacgat
    13681 gccgcacttg aggctcatga gggtctttcg tcgcggcacg gcgccagggg cggcgtcgtc
    13741 gtgcgcggtt cgtggaaaac ccgggatttt agctggtcag ggcatcttca ccttggcctc
    13801 cctacaatgc ggaatgtcca agatcgacgt acgaatccac ggcagcggca tcgtcgggcg
    13861 cgccctggcg ctgaagctgg cccgccaggg actgggcgtg ggcatcgacg cccgtccgtc
    13921 ggcgccggcc ggcggcgagc agcgggccga cgtccgcgcc tacgcgctca acgcggcctc
    13981 ggtcgagctg ctgcgcgagc tcaaggtgtg ggacggcatg gcgccggacg cccgcaccgc
    14041 cgtctacgac atgcgcatcg ccggcgacgg ccagggcgcg ctggcgttct ccgcctggca
    14101 gcagacggtg cccgcgctgg cctggatcgt cgacgccgcc gaactcgagc aggtgctcga
    14161 gacggcgatc cgcttcgcgc ccgcggtgca cgcggtcgac gccgacgcga aggcgccgct
    14221 gacggtcatc gccgaaggca aggccgcggc ggcgcgcgac aggctcggcg tgacgttcga
    14281 gcgccattcg tatggccaca gcgcgctcgc ggcgcgcgtg gtgtcggacg tgccgcacgc
    14341 cggcgtggcg cgccagtggt ttcgcgcgcc cgacgtgctc gcgctgttgc cgttcgaccg
    14401 cccgcagccc gagtgttcgt acggcgtcgt gtggtcgatg ccccaggccg aggccgagcg
    14461 cctgctgcag tcgagcgacg ccgacttcga cgatgcgctc gatgccgcca ccggcggtgc
    14521 ggccggcaag ctgcacgtgc ggggtccgcg cgccagctgg ccgctgatgc aggccagggc
    14581 ggccgaggtc gtcggcccgg gctgggcgct ggtcggcgac gcggcgcatg tcatccatcc
    14641 gctggccggg cagggcctga acctggggct ggccgacgtc gtggcgctgg ccaaggtcgt
    14701 cgccgagcgc gaggcctggc gcgcgctggg cgacgaggcg ctgctgcgcc gctacgcccg
    14761 cgcccgcctg tggcccaccc gcgcgatggc cggcgccgtc gacggcctgt ggcagctgtt
    14821 ctgcgcgccg gcgcccggcc tgcgcgaact gcgcaaccac ggcatgtcgc tggtcgaccg
    14881 cctcagcccc ctgaaacgct tcctcgtctc gcgggccctc ggctcgtgaa cgccttttcg
    14941 ttcgagactg ccatgaccaa gttcgctcgc gccctgcgcc tcctccttgc cggttcgctc
    15001 gccctcgcct gcctcgcggc ctggtccgac gacgcggcca tccgccgcag ctggaaccag
    15061 cgccatccca acgggccggc catcgacgag atcaccagga cgcccatcgc cggcctgtac
    15121 gagctgcgca tcggcgccga cctcgtgtac tccgacgagc agggcaacta cctgatctac
    15181 ccgggccgcg acgccggcaa cggccaggtg cccgacggcc acatcttcga cgccaggaag
    15241 aacgtcgaca tcaccgacca gcggctggac aagctgatgg aaaaggaagt gcccaagctg
    15301 ccgtacggcg atgcgctggt gttcaagcag ggcaacggcg cacgcaagat ggtggtgttc
    15361 gaggatccga actgccacta ttgcaaggac gccgagcgca gcttcatgca gctcaaggac
    15421 gtgacgatct acacgttcct gatcccgatc ctgggcgatg actcggtggc caaggcgcgc
    15481 acgatctggt gctcgaagaa caacgcgcag gtctggcgcg actggatggt caacggcgtg
    15541 cttcccccgc gtccgatggg cgcctgcgac atgaaggtgc tggatcgcaa cctcgagctc
    15601 gccgcgcgcc atcacctgaa ctacacgccg gccatcatct tcgaggacgg ctcccgcttc
    15661 gcgggcaacg ccgacctggc gcacctgacc aagcggctgg atgaagtcgc cgccgccagg
    15721 aagagctgaa cggccccgcg gccacaatag cccctcgatg acaccgaccc tccgccgccc
    15781 gtcgcgcgcg gcgttcccgc tgcaatgatc cattaccgca tcgaggtcgc cgacgcggcg
    15841 gcccaccagt tcaaggtcac gctgaccatc gccgaccccg ctcccgtgca gcagctgagc
    15901 ctgccggtgt ggatccccgg cagctacatg gtgcgcgagt tctcgcgcca cctgagcctg
    15961 ctggacgcgc gccagggcgc gcagccgcgg ccgatcgagc agctcgccaa ggatcgctgg
    16021 agcgtggcga cgtcgggacg cgcggcgctg gtcgtcacct acttcgtcta cgcgttcgat
    16081 ccgtcggtgc gcacggcgtg gctcggcgac gaccgcggct tcttcaacac cacgagcctg
    16141 tgcctggccg tcgacggccg taccgccgag ccgcaccgcg tcgagctgca tcgcctgccg
    16201 gcgggctggg acgtcgccac gggcatgacg cgcatcgatc ccggcgcgag gacggccggc
    16261 aaggccaagg gcgccaaggc cgtgccggcg cgcacgcagg tgttcgagtc ggccgactac
    16321 gacgagatgg ccgaccaccc gttcgagctc ggcccgttct ggcgcggcga gttcacgtcg
    16381 ggcggcgtgg tgcacgagtt cgtcgtcgcc ggcgcc
//
LOCUS       DATCRO010000450         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_27259, whole genome shotgun sequence.
ACCESSION   DATCRO010000450 DATCRO010000000
VERSION     DATCRO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27259"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..509
                     /locus_tag="VIN75_12215"
     CDS             33..509
                     /locus_tag="VIN75_12215"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEY8974979.1"
                     /translation="MSSFRLVGLAAENFEPLFAMSDDELAALGARRVVADAPVGFPCR
                     VSLADAQAGDELLLLPFEHLSTRSPYRASGPVFVRRGVPRAVLAPGEIPPYVTRRAMS
                     VRAYDANDMMIDADVCDGKEARATIERLLADDAVAFIHLHNAKRGCFSCRVERVAD"
     gene            complement(517..1017)
                     /locus_tag="VIN75_12220"
     CDS             complement(517..1017)
                     /locus_tag="VIN75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974980.1"
                     /translation="MASDIDDNGVQDDADERVHEARLWVDNGWTARVIKNEDDDGWAV
                     SMTRDGEREPALVGPWTMGRDKKNPKPLDVNAFNTLVKTAAEVIRRHEQAVHAAMHKQ
                     FQVTTDSGRVLVTLDIVPDEFDPHALLAAQDEFGERLAQVKVRPDFKLTLASATAWVE
                     AGYRKP"
     gene            1118..1549
                     /locus_tag="VIN75_12225"
     CDS             1118..1549
                     /locus_tag="VIN75_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8974981.1"
                     /translation="MDLSLSTWWWIVAGLLVAAELATGTFYLLMVAIGCAAGALAAHA
                     ELAFLAQALVSAAVGGGLVLAWHHLRARSPQVLPPQANRDINLDIGEQLQVDGWNADG
                     TARVPYRGSAWNVRYIGDGPAAPGPHVIAEVRHNELAVRRA"
     gene            1590..2516
                     /locus_tag="VIN75_12230"
     CDS             1590..2516
                     /locus_tag="VIN75_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEY8974982.1"
                     /translation="MDFQILALVVVVVVVTFIYRAVKVVPQQSAWVVERLGKFRAVLP
                     PGPNILIPFVDNVAYRHSLKEIPLDVPSQVCITRDNTQLQVDGILYFQVTDAMRASYG
                     SSNYVLAITQLCQTTLRSVIGKMELDKTFEERDLINGMVVNALDEAASNWGVKVLRYE
                     IKDLTPPAEILRAMQAQITAEREKRALIAASEGRRQEQINIATGQREAAIARSEGEKQ
                     AEINKAQGEAAAIVAVAEATADAIRKVAEAIRAPAGEQAVQLKVAERAIDAYQELAKT
                     NNTMIVPGNMTEVSGLIASAMAVMKGNVVKPG"
     gene            2820..3092
                     /locus_tag="VIN75_12235"
     CDS             2820..3092
                     /locus_tag="VIN75_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HEY8974983.1"
                     /translation="MTDPDAPPPAQPRNPLHGLTLEAILTALVAAYGWDGLDERIPLR
                     CFAFDPSIKSSLKFLRKTPWAREKVESLYLFMLRDQRREEQGRERR"
     gene            complement(3156..>3913)
                     /locus_tag="VIN75_12240"
     CDS             complement(3156..>3913)
                     /locus_tag="VIN75_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8974984.1"
                     /translation="SLTMGGSDRALMLAVPGVAVLAAFALPTLKRSAGSAIDWFSVFF
                     FTGLAIAIWVFYIAMMTGTPAKPALRIAHLLPGFEPRFSAPLLALAVAGMAAWLALVR
                     WRTARVQHALWKSLVLPASGVALSWLLLLTLGLPVIDYARSYRPWVYLVAQHVPNGVC
                     VAAQLPRSALAALENYSNWRIDAQGDVVKNSECPYLLVDENRHSPVQAPPGWSLVAHL
                     HRPSERDESTAVFRKDNAPTPRPADFGGVASAR"
BASE COUNT          597 a         1381 c         1390 g          545 t
ORIGIN      
        1 ggccatgttc ctccactgcc gcaaagcacg ccatgtcctc gttccgcctg gtcggcctcg
       61 ccgccgagaa cttcgaaccc ttgttcgcca tgagcgacga cgagctcgcc gcgctcggcg
      121 cgcgccgcgt cgtcgcggac gcgcccgtcg gcttcccatg ccgcgtgtcg ctggccgacg
      181 cccaggccgg cgacgagctc ctgctgctgc cgttcgagca cctgagcacg cgctcgccgt
      241 atcgcgcgag cgggcccgtg ttcgtgcgcc gcggcgtgcc gcgcgccgtg ctggcgccgg
      301 gcgagatccc gccgtacgtg acgcggcgcg cgatgtccgt gcgcgcctac gacgcgaacg
      361 acatgatgat cgacgccgac gtgtgcgacg gcaaggaggc gcgcgccacg atcgagcgcc
      421 tgctggccga cgacgccgtc gccttcatcc acctgcacaa tgccaagcgc ggctgcttct
      481 cgtgccgcgt cgagcgcgtg gcggactgag ccgccctcag ggcttgcggt agcccgcctc
      541 gacccacgcc gtggcgctgg ccagcgtcag cttgaagtcg ggccgcacct tcacctgcgc
      601 gaggcgctcg ccgaactcgt cctgcgcggc gagcagggcg tgcgggtcga actcgtcggg
      661 cacgatgtcg agcgtgacca gcacgcggcc cgaatcggtc gtgacctgga actgcttgtg
      721 catcgccgcg tgcaccgcct gctcgtggcg ccggatgacc tcggccgccg tcttgacgag
      781 cgtgttgaac gcgttgacgt ccagcggctt cggattcttc ttgtcgcggc ccatcgtcca
      841 cgggcccacc agcgcgggct cgcgttcgcc gtcgcgcgtc atcgacacgg cccagccgtc
      901 gtcgtcctcg ttcttgatga cgcgggccgt ccagccgttg tccacccaca ggcgcgcctc
      961 gtgcacgcgc tcgtccgcgt cgtcctggac gccgttgtcg tcgatatcgg atgccatggc
     1021 ggcatgatac gggtcgcact ggctcacgac gtgcgccaga cggggcgaaa agccgcaaca
     1081 atggcgcatc gttcgaaaga ctgcggaggg cggcgcgatg gacctgagcc tgtcgacctg
     1141 gtggtggatc gtcgccggcc tgctggtggc ggcggaactg gccacgggca ccttctacct
     1201 gctgatggtg gccatcggct gtgccgcggg cgcgctcgcc gcgcacgccg agctggcgtt
     1261 ccttgcgcag gcgctcgtct ccgccgcggt gggcggtggc ctggtgctcg cgtggcacca
     1321 cctgcgcgcg cgctcgcccc aggtcctgcc gccgcaggcc aaccgcgaca tcaacctgga
     1381 catcggcgag caactgcagg tcgatggctg gaacgccgac ggcaccgcgc gcgtgcccta
     1441 tcgcggctcg gcctggaacg tgcgctacat cggcgacggg ccggccgcgc ccggcccgca
     1501 cgtgatcgcc gaggtgcgcc acaacgaact ggccgtgcgt cgcgcctgag cccggcaccc
     1561 cccgttcctt caaccgagaa cccgcaatga tggatttcca gattctcgcg ctcgtcgtgg
     1621 tggtggtcgt cgtcaccttc atctaccgcg ccgtcaaggt ggtgccgcag cagagcgcgt
     1681 gggtggtcga gcgcctgggc aagttccgcg cggtgctgcc gccggggccc aacatcctga
     1741 tcccgttcgt cgacaacgtc gcctacaggc actcgctcaa ggagatcccg ctggacgtgc
     1801 ccagccaggt ctgcatcacg cgcgacaaca cgcagctgca ggtcgacggc atcctgtact
     1861 tccaggtgac cgacgcgatg cgcgcgagct acggttccag caactacgtg ctcgcgatca
     1921 cgcagctgtg ccagaccacg ctgcgttcgg tcatcggcaa gatggagctg gacaagacct
     1981 tcgaggagcg cgacctgatc aatggcatgg tcgtcaacgc gctggacgag gcggcgtcga
     2041 actggggcgt caaggtgctg cgctacgaga tcaaggacct gacgccgccg gccgagatcc
     2101 tgcgcgcgat gcaggcgcag atcaccgccg agcgcgagaa gcgcgcgctg atcgccgcgt
     2161 ccgagggccg gcgccaggaa cagatcaaca tcgccaccgg ccagcgcgag gcggccatcg
     2221 cgcgctccga gggcgagaag caggccgaga tcaacaaggc gcagggcgag gccgcggcca
     2281 tcgtcgccgt ggccgaggcc accgcggacg ccatccgcaa ggtcgccgag gccattcgcg
     2341 cgccggccgg cgagcaggcc gtgcaactga aggtggccga gcgcgccatc gatgcctacc
     2401 aggagctggc caagaccaac aacacgatga tcgtgccggg caacatgacc gaggtctcgg
     2461 gcttgatcgc gagcgcgatg gcggtgatga agggcaacgt cgtcaagccg ggctgaggcg
     2521 cgcccggggt gggtcgggca ggtggctgct gaaaagacac gggcgccgcc acgatgagtg
     2581 ggagcgcccg cgggtctggt cgagacctgg ctacatcacg tagggaggcc cctctcggac
     2641 caagagaatg aagacatgtc acctcctttc gtagcaactg gggcctccag ggtacctcaa
     2701 gacgggcggc cgggtcgtcc gtggtggcgt cggacggcga ggctgctcga cgggacgccg
     2761 gtgattcctg tcggcaacga ggcttcgccg cgacggcggc cggcggcgac aatcgccgca
     2821 tgaccgaccc cgacgcgccg ccgcccgcgc agccccgcaa cccgctgcac ggcctcacgc
     2881 tggaggccat cctcaccgcc ctggtggcgg catacggctg ggacggcctg gacgagcgca
     2941 tcccgctgcg ctgcttcgcc ttcgacccca gcatcaagtc cagcctgaag ttcctgcgca
     3001 agacgccgtg ggcgcgcgag aaggtcgaga gcctgtacct gttcatgctg cgcgaccagc
     3061 ggcgcgagga acaggggcgc gagcggcgct gatggcagcg ggctcccgcg cgtgggcgcg
     3121 ggcgggcgtc acgcgcgcga gcgcgcgggt acgcgtcagc gcgcggacgc gacgccgccg
     3181 aagtcggctg gacgcggcgt gggagcgttg tccttcctga acacggccgt cgactcgtcg
     3241 cgctccgacg gccggtgcag gtgggcgacc agcgaccagc ccggcggcgc ctggacgggc
     3301 gagtgccggt tctcgtcgac cagcaggtag gggcactcgc tgttcttgac gacgtcgccc
     3361 tgcgcgtcga tgcgccagtt gctgtagttc tccagcgcgg ccagcgccga gcgcggcagc
     3421 tgcgcggcga cgcacacgcc gttgggcacg tgctgcgcga cgaggtagac ccaggggcgg
     3481 tagctgcgcg cgtagtcgat caccggcagg cccagcgtca gcagcagcag ccagctgagc
     3541 gcgacgcccg aggccggcag caccagcgac ttccacaggg cgtgctgcac gcgcgccgtg
     3601 cgccagcgca ccagcgccag ccaggcggcc atgccggcca cggccagcgc cagcagcggc
     3661 gccgagaagc gcggctcgaa gccgggcagc aggtgcgcga tgcgcagcgc gggcttggcg
     3721 ggtgtgcccg tcatcatcgc gatgtagaac acccagatcg cgatggccag gccggtgaag
     3781 aagaacaccg agaaccagtc gatcgccgag cccgcggagc gcttgagcgt gggcagcgcg
     3841 aacgcggcca gcacggccac gccgggcacc gccagcatca gcgcgcggtc ggagccgccc
     3901 atcgtcagcg agg
//
LOCUS       DATCRO010000451         5533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_916362, whole genome shotgun sequence.
ACCESSION   DATCRO010000451 DATCRO010000000
VERSION     DATCRO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916362"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VIN75_12245"
     CDS             complement(<1..989)
                     /locus_tag="VIN75_12245"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY8974985.1"
                     /translation="MPGPSDPSFDTLALHAGAAPDPATGARVTPLHLSTSFVFESSEH
                     AASLFNMERAGHVYSRISNPTTAVFEERVAALEGGIGAIATASGQAALHLAIATIAGA
                     GSHIVASSALYGGSHNLLAYTLPRFGIETTFVKPRDIDGWRAAIRPNTRLLLGESLGN
                     PGLDVLDIPRVSAIAHDAGVPLLVDATFTTPWLQQPFKLGADLVFHSATKFLSGHGTV
                     VGGVLVDSGRFDWERSGRFPELTTPYAGFHDMVFTEESTVGAFLLRARREGLRDFGAC
                     MSPHTAWLILQGLETLPLRMERHVANARKVARFLAAHPLVAQAGYPDLESHPD"
     gene            1122..1586
                     /locus_tag="VIN75_12250"
     CDS             1122..1586
                     /locus_tag="VIN75_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8974986.1"
                     /translation="MKVSDILRVKGNTLFTVSPDQPLHEAVVTMAEHDIGSLVVMDHG
                     ELTGMLTFREVIRATVRQGGTLGNVTIRSVMDDAPLTCTPETEIDEVRRMMLGRHARY
                     MPVLNGQTLMGVISFYDVARTVVEAQDFENRMLKAYIRDWPSEEDAKVQTEL"
     gene            complement(1614..3293)
                     /locus_tag="VIN75_12255"
     CDS             complement(1614..3293)
                     /locus_tag="VIN75_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8974987.1"
                     /translation="MLTHHHFRPRLLDSLRGYRRADFLADLGAGLTVGVVALPLAMAF
                     AIASGVKPEQGLFTAIVAGLLISGLGGSSVQIGGPAGAFIVIVYGIVQRFGVPNLLTA
                     TMMAGVLMLVMGWMRVGALVRQMPVSIIAGFTNGIAVLIALSQLKDFLGLPIASMPSG
                     FFAQAATVVELAPRANLQALLLGGLCLAGLLFWTSLSVRDNPLRQDSGVDRWVKFAAR
                     IPGPIIALVTLTALSSLLELHVETIGSRFGGIPQSLPSLRAPVLSWDAAQQLFVPALT
                     IALLGAVESLLCARVADGVAQVRRHEPNQELMAQGVANLVAPLFGGIPATGTIARTLT
                     NIRAGARTPVAGILHAITLMAVMLVAAPLAARVPLPVLSGILLYVAWNMLEPREFLRL
                     RKDGVEYGAKFLCTFVLTVVLDLTVAVEVGLALACVLFVYRMGTLFGLEADTHSGAAG
                     DVRVFRVYGALFFGAAGKVESLADEVPAGVRWVILEANHLLSIDSSGQSALEALGREL
                     ARRDIGWIVCGLHRQPLQELDPAATAHWAAPPLLMPDLAAALHRTEASAPA"
     gene            complement(3454..>5533)
                     /locus_tag="VIN75_12260"
     CDS             complement(3454..>5533)
                     /locus_tag="VIN75_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8974988.1"
                     /translation="LQRERLANRLIDRSPNLVCVLDPRGALRHMNGTGRHWLRVSHPQ
                     IAGRRLDEIPELGLDAVQAHAFQAAIEQAVAGTILTHELTVRRPDGVAMTLELSIRAI
                     PRSGDAPPNLLVEGRDITQRKSVEDKLLLAAAVFEQAREGFVIADRDGRVVTVNQAFC
                     DISGYDADELQGQRVTALGLGIGRREVRRQVRSALQTAGHWQGEVRTSRKDGSLYTCW
                     AAVTQQRDAHGAVTHYIGILNDITQFREVESNLVRLAHVDTLTDLPNRSLLADRVGQL
                     TSVSRRDHRPFALMFMDLDQFKNINDTLGHSVGDALLSEVARRLQGTLREVDTVARLG
                     GDEFAVLLPGADAAGAELVAAKLLERLAEPCMVQGHELSMTMSIGVACYPSDGDDYEI
                     LSRNADTAMYRAKHEGKATWRFFAAGMQQRSARQLQLESALRRALERGELLLHYQPQL
                     DGNGEQLVGVEALLRWKHPEFGFVSPAEFIPLAETSGQIVAIGEWVLRTAVAQLKAWM
                     DAGVPPMVVAVNLSAVQFRHVGLPNAVARALSDAGLPAQYLELELTESVTANPAAAIS
                     MMDALHALGVRLSLDDFGTGYSSLSYLKRFPLHTLKIDQSFVRDIDTDPDDRAIVQAI
                     IQMARALNLKTIAEGVETDAQHQFLRREGCDMMQGYRFCRPMDAAALEMWIAGREAAS
                     TANVVPMIAA"
BASE COUNT          850 a         1883 c         2029 g          771 t
ORIGIN      
        1 tggtcggggt gcgactcgag gtcggggtag ccggcctgcg ccaccagcgg atgcgccgcg
       61 aggaaacggg cgaccttgcg ggcgttggcg acatggcgct ccatgcgcag cggcagcgtc
      121 tccagcccct gcaggatcag ccaggccgtg tgcgggctca tgcaggcgcc gaagtcgcgc
      181 aggccctcgc ggcgcgcgcg cagcaggaag gcgccgacgg tgctctcctc ggtgaacacc
      241 atgtcgtgga agccggcgta gggcgtggtc agctcgggga agcgcccgct gcgttcccag
      301 tcgaagcggc cggagtccac gagcaccccg ccgacgacgg tgccgtgccc ggacaggaac
      361 ttggtggccg agtggaacac caggtcggcg cccagcttga acggctgctg cagccacggc
      421 gtcgtgaacg tggcgtcgac cagcagcggc acgcccgcgt cgtgcgcgat ggccgacacg
      481 cgcgggatgt ccagcacgtc cagtcccggg ttgcccagcg actcgccgag cagcagccgc
      541 gtgttcgggc ggatggcggc gcgccagccg tcgatgtccc gcggcttgac gaaggtcgtc
      601 tcgatgccga agcggggcag cgtgtaggcc agcaggttgt gcgaaccgcc gtacagcgcc
      661 gagctggcga cgatgtgcga gccggcgccc gcgatggtgg cgatggccag gtgcagcgcg
      721 gcctggccgc tggccgtggc gatcgcgccg atgccgccct cgagcgcggc cacgcgttcc
      781 tcgaacacgg ccgtggtcgg gttggagatg cggctgtaga cgtggcccgc gcgctccatg
      841 ttgaacagcg aggccgcgtg ctcgctcgat tcgaagacga aggacgtgct caggtgcagc
      901 ggcgtgacgc gtgcgccggt ggccgggtcg ggcgcggcgc cggcgtgcag ggcgagggtg
      961 tcgaaggacg ggtcggacgg gccgggcatg tgtgctttcc gcggggactg ttgcaggggt
     1021 gccgagcatc acccaagcca gggatgaacc gcgacaccaa cgccacgatt gtgtgctatg
     1081 ttgaagccct gttcccactg attccccgag gaggcgttgc catgaaagtc agcgacatcc
     1141 tgcgtgtcaa gggcaatacc ttgttcaccg tctcgcccga ccagccgctc cacgaagccg
     1201 tcgtcacgat ggccgagcac gacatcggtt cgctggtcgt catggatcac ggcgagctga
     1261 ccggcatgct caccttccgc gaagtcatcc gcgccactgt ccggcagggc ggcacgctgg
     1321 gcaacgtcac catccgctca gtgatggacg acgcgccgct cacctgcacg cccgagaccg
     1381 agatcgacga ggtccgccgc atgatgctcg gccggcacgc gcgctacatg cccgtgctca
     1441 acggccagac gctgatgggc gtgatctcgt tctacgacgt cgcccgcacg gtcgtcgagg
     1501 cgcaggactt cgagaaccgc atgctcaagg cctacatccg cgactggccg tccgaggagg
     1561 acgccaaggt ccagaccgag ctctgaggcc caggcggccg gggcgggccg ccctcaggcc
     1621 ggcgcgctcg cttcggtgcg gtgcagcgcg gcggccaggt cgggcatcag caggggcggc
     1681 gcagcccaat gcgccgtcgc cgccgggtcg agttcctgca gcggctggcg atgcagtccg
     1741 cagacgatcc agccgatgtc gcgcctcgcg agttcgcgcc ccagcgcctc gagcgcgctc
     1801 tggccggagc tgtcgatcga cagcaggtgg ttcgcctcca ggatcaccca gcgcacgccg
     1861 gccggcacct cgtcggcaag cgactccacc ttgccggcgg caccgaagaa cagggcgccg
     1921 tagacgcgga acacgcgcac gtcccccgcc gcgccggaat gggtgtccgc ctccagtccg
     1981 aacagcgtgc ccatgcggta gacgaacagc acgcaggcca gtgcgagacc gacttcgacc
     2041 gccacggtca ggtccagcac cacggtcagc acgaaggtgc acaggaactt ggcgccgtac
     2101 tcgacgccgt ccttgcgcag gcgcaggaat tcgcgcggct ccagcatgtt ccacgccaca
     2161 tacagcagga tccccgacag gaccggcagc ggcacgcggg cggccagcgg cgcggccacc
     2221 agcatgaccg ccatcagggt gatcgcgtgc aggatgccgg cgaccggggt acgggcgccg
     2281 gcgcgtatgt tggtcagcgt acgggcgatc gtcccggtcg cggggatgcc gccgaacagc
     2341 ggcgccacga gattggcgac gccctgtgcc atcagctcct ggttgggctc gtggcggcgc
     2401 acctgggcga caccgtcggc gacgcgcgcg cagagcagcg actcgacggc gcccagcagc
     2461 gcgatggtca acgccggcac gaacagctgc tgcgcggcgt cccacgacag gaccggcgcc
     2521 cgcagcgacg gcagcgactg cggaatgccc ccgaagcggc tgccgatggt ctcgacatgc
     2581 agttcgagca ggctcgagag cgccgtgagc gtgaccagcg cgatgatggg ccccgggatg
     2641 cgggctgcga acttgaccca gcggtcgacg ccgctgtcct gtcgcagcgg attgtcgcgc
     2701 accgacaggc tcgtccagaa cagcaggccg gccaggcaca ggccgcccag cagcagggcc
     2761 tgcaggttgg cgcgcggcgc cagttcgacc accgtggcgg cttgcgcgaa gaaaccggac
     2821 ggcatcgagg cgatcggcag tccgaggaag tccttgagct gggacagcgc gatcagcacc
     2881 gcgatgccgt tggtgaatcc cgcgatgatg gacaccggca tctggcgcac cagcgcgccc
     2941 acgcgcatcc agcccatgac gagcatcagc acgcccgcca tcatcgtggc cgtcaggagg
     3001 ttgggcacgc cgaagcgttg aacgatcccg tacacgatga cgatgaaggc gccggcgggg
     3061 ccgccgatct ggacgctgga tccgcccagg ccggagatca gcaggcccgc gacgatggca
     3121 gtgaacagcc cctgttccgg tttgactccg gaggcgatgg cgaacgccat ggccagcggc
     3181 agggcgacga cgccgacggt caggccggcg ccgaggtcgg cgaggaaatc cgcccgtcga
     3241 tagccgcgca gcgagtcgag caggcgcggc ctgaagtgat gatgggtgag catgtggcga
     3301 tcctggcaac ggttgcagtg aaggactgaa ccggagcggc aggcggaccg cagtggtgca
     3361 cgcgcgccat gaagcgccgc cgccgcgagg gcagccaggc acgggaggcg cgtggaacgg
     3421 acatgcctcg atgttacgac gaggtgcggc tgctcaggcc gcgatcatgg gcacgacgtt
     3481 ggccgtcgac gccgcctcgc ggccggcgat ccacatctcc agcgcggcgg cgtccatcgg
     3541 gcggcagaag cggtagccct gcatcatgtc gcagccctcg cggcgcagga actggtgctg
     3601 ggcgtcggtc tccacgcctt cggcgatggt cttcaggttc agcgcgcgcg ccatctggat
     3661 gatggcctgc acgatggcgc ggtcgtccgg gtcggtgtcg atgtcgcgca cgaaggactg
     3721 gtcgatcttg agcgtgtgca gcgggaagcg cttgaggtag ctcagcgacg agtagccggt
     3781 gccgaagtcg tccagcgaca ggcgcacgcc cagcgcatgc agcgcgtcca tcatcgagat
     3841 cgcggccgcc gggttggccg tcacgctctc ggtcagttcc agctccaggt actgcgccgg
     3901 caggccggcg tcgctcagcg cccgcgccac tgcgttgggc aggccgacgt ggcggaactg
     3961 gacggccgac aggttcacgg ccaccaccat cggcggcacg cccgcgtcca tccacgcctt
     4021 gagctgcgcg acggcggtgc ggagcaccca ttcgccgatc gccacgatct ggccgctggt
     4081 ctcggccagc gggatgaact cggcgggcga gacgaagccg aactccgggt gcttccagcg
     4141 cagcagcgcc tcgacgccca ccagctgctc gccgttgccg tccagctgcg gctggtagtg
     4201 cagcagcagc tcgccgcgct ccagcgcgcg gcgcagcgcc gattccagct gcagctggcg
     4261 cgccgagcgc tgctgcatgc cggcggcgaa gaagcgccac gtcgccttgc cttcgtgctt
     4321 ggcccggtac atcgcggtgt cggcgttgcg cgacaggatc tcgtagtcgt cgccgtcgga
     4381 cgggtagcag gcgacgccga tcgacatcgt catgctcaac tcgtggccct gcaccatgca
     4441 cggctcggcc aggcgctcga gcagcttggc ggccaccagc tcggcgcccg cggcgtcggc
     4501 gccgggcagc aggacggcga actcgtcgcc gcccaggcgg gcgacggtgt cgacctcgcg
     4561 cagcgtgccc tgcaggcggc gcgcgacctc gctcagcagc gcgtcgccga cgctgtgccc
     4621 gagcgtgtcg ttgatgttct tgaactggtc caggtccatg aacatcagcg cgaatggccg
     4681 atggtcgcgg cgcgacacgc tggtcagctg cccgacgcgg tcggccagca ggctgcggtt
     4741 gggcaggtcg gtgagggtgt ccacgtgcgc gaggcgtacc aggttgctct cgacctcgcg
     4801 gaactgcgtg atgtcgttga ggatgccgat gtagtgcgtg accgcgccat gcgcgtcgcg
     4861 ctgctgcgtc acggcggccc agcaggtgta gaggctgccg tccttgcgcg aggtgcgcac
     4921 ctcgccctgc cagtggccgg ccgtctgcag cgcgctgcgc acctggcggc gcacctcgcg
     4981 gcggccgatg cccaagccca gcgcggtcac ccgctggcct tgcagctcgt cggcgtcgta
     5041 gccgctgatg tcgcagaagg cctggttgac ggtgacgacg cggccgtcgc ggtcggcgat
     5101 gacgaagccc tcgcgcgcct gctcgaacac ggcggcggcc agcagcagct tgtcctcgac
     5161 cgacttgcgc tgggtgatgt cgcggccctc gaccaggagg ttgggcgggg cgtcgccgct
     5221 gcgcgggatc gcccggatcg acagctccag cgtcatcgcc acgccgtcgg ggcgccggac
     5281 ggtgagctca tgggtgagga tcgtgccggc gacggcctgc tcgatcgcgg cctggaacgc
     5341 gtgcgcctgg acggcgtcca ggcccagctc ggggatctcg tcgaggcgac ggccggcgat
     5401 ctgcggatgg ctgacgcgca gccagtggcg gccggtgccg ttcatgtggc gcagggcgcc
     5461 gcgggggtcg agcacgcaca ccaggttcgg gctgcggtcg atcaggcggt tggccaggcg
     5521 ctcgcgctgc agc
//
LOCUS       DATCRO010000452         5677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_994966, whole genome shotgun sequence.
ACCESSION   DATCRO010000452 DATCRO010000000
VERSION     DATCRO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994966"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VIN75_12265"
     CDS             complement(<1..712)
                     /locus_tag="VIN75_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8974989.1"
                     /translation="MKSLRPVLCAIALASLAGVAAPATAPEPSVLAVARVAQFDTLDP
                     QRQFDFYSDQVLRQVYSTLLTYAYLERPYKLEPDLLEAMPTLGADKVTLTFRLRKGVR
                     FVDNACFPGGRGRELTADDVLYSLKRYADANVNNKSYFAMQGAVVGLDEFRAATAKAG
                     PDVDVSTMDVAGLRKIDKYAFTIRLTHENPLFLYALTLSSTDIVPVEAVRMYKERLSV
                     TPVGTGPFMATGPLDRKGT"
     gene            complement(730..1695)
                     /locus_tag="VIN75_12270"
     CDS             complement(730..1695)
                     /locus_tag="VIN75_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8974990.1"
                     /translation="MTAAPLLQAEGLSVAFPVRGGLLRREVARLRAVDRVDVQVAAGE
                     VLGIVGESGCGKTTLGRALVGLIEPDAGTLRVEGRDVSTLSREERRALTRDVQMVFQD
                     PLASLNPRKRVRQALALPFEAHGLPVDAARLRELAALVGLAPEHLDRWPHELSGGQRQ
                     RVGIARALALQPKVVVLDEPLSALDMSIQSQVLNLLVDLQERLALSYVFVSHDLSVVA
                     WLCDRVAVMYLGRVVETAPAAALFASPLHPYTQALLAAVPDPVPRRGRAGHLLEGDVP
                     SPLHAPSGCAFHTRCALAQPRCRESVPELRRMVSTDMHLAACHLL"
     gene            complement(1757..2755)
                     /locus_tag="VIN75_12275"
     CDS             complement(1757..2755)
                     /locus_tag="VIN75_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8974991.1"
                     /translation="MSEPPLLEIDGLVTSFRTPRGPVRAVDGVSLRVERGQTVCVAGE
                     SGCGKSVTALSVMGLLPEAARVEAGAIRLRGVDLLALPPRERRGLRGRALAMVFQEPM
                     TALNPVLTIGAQVAEVFAIHGTATRREARDRTIALLRQVKIPDPERRVDDYPHQLSGG
                     MKQRVMIALALANRPGLLIADEPTTALDVTIQAQVLHLLRELQRELGTGIVFVTHDLA
                     VVAEVADRVVVMYGGRVVEEGSVFDVLERPAHPYTIGLLGARPRPGQTRRGGARLDVI
                     PGTVPAAGARIDGCRYQSRCPRAQARCRTEAPVLARVTPAVGDHAAACHFAEGGRA"
     gene            3705..4709
                     /locus_tag="VIN75_12280"
     CDS             3705..4709
                     /locus_tag="VIN75_12280"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8974992.1"
                     /translation="MLLLALASCASKDAQPPAPAGIGAADPWMRPAAVPFPADNAPNA
                     SRVALGRDLFFDTRLSGSSMISCATCHNPALGWSDGQPTAVGDGAKRLGRKTPTVLNT
                     AYQPLQMWDGRMKSLEDQALGPIGSTAEMNMPLDRMVEHLRAIPGYAPMFEQAYPGEG
                     VTQGTVAKAIASFERTVVSTTSPFDRWRRGDEQAVDASAKRGFALFQGKARCTLCHQG
                     FNFTDNGYHNIGIRSLSGAEDDGRFAHRKLKVLKGAFKTPTLRDVELTAPYMHNGIYR
                     TLEEVVDHYDRGGDVKDNLSPNIQPLGLTAEEKHDLVAFMKSLTGQPMVVEVPHLPPG
                     "
     gene            4748..5065
                     /locus_tag="VIN75_12285"
     CDS             4748..5065
                     /locus_tag="VIN75_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8974993.1"
                     /translation="MKALLALLSCLAVLSPAFAAQEVVVAQKNKSFSQPRITIHAGDK
                     VTFRNDDAFVHNVFSLTDAQPFDLGTYTQGQAKSVTFAKAGTYDIECAIHPEMKLTVV
                     VAP"
     gene            5062..5427
                     /locus_tag="VIN75_12290"
     CDS             5062..5427
                     /locus_tag="VIN75_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8974994.1"
                     /translation="MKTRAVAALAALLVAGVARAAGTDEAIARGGKLFGDRCALCHGS
                     NADGKGQLAKMLDPKPANLRASLLDRGARESIIRHGGAAVGRSPAMPRWEVEFDEQQL
                     DDVIAYVDSIVPAHSGNTP"
     gene            5424..>5677
                     /locus_tag="VIN75_12295"
     CDS             5424..>5677
                     /locus_tag="VIN75_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8974995.1"
                     /translation="MKRSMIAWLLALLPLLVAAAPEDGEPPLAGLGGPIDLVDQGGHS
                     FHLAQLGRRRALVFFGFTGCGTMCPTAMLTARQVLARTPAG"
BASE COUNT          874 a         1984 c         1973 g          846 t
ORIGIN      
        1 gcgtgccctt gcgatcgagc ggcccggtcg ccatgaacgg accggtgccc accggcgtga
       61 cgctgagccg ctccttgtac atgcgcaccg cctcgacggg cacgatgtcg gtggacgaca
      121 gcgtcagcgc gtacaggaac agcgggttct cgtgcgtcag cctgatcgtg aacgcgtact
      181 tgtcgatctt gcgcaggccg gcgacgtcca tcgtcgagac gtccacgtcc ggacccgcct
      241 tggcggtcgc ggcgcggaac tcgtccaggc cgacgaccgc gccctgcatc gcgaagtagc
      301 tcttgttgtt gacgttggcg tcggcgtagc gcttgagcga gtacagcacg tcgtcggcgg
      361 tcagctcgcg gcccctgccg ccgggaaagc aggcgttgtc gacgaagcgc acgcccttgc
      421 gcagcctgaa cgtcagcgtg accttgtcgg cgccgagcgt gggcatcgcc tccagcaggt
      481 cgggctcgag cttgtagggc cgctcgagat acgcgtaggt cagcagcgtg ctgtagacct
      541 gccgcagcac ttggtcgctg tagaagtcga actggcgttg cgggtccagg gtgtcgaact
      601 gcgcgacgcg ggcgacggcc agcaccgagg gctcgggcgc ggtggccggc gcggcgacgc
      661 cggccaggct cgccagggcg atcgcgcaga ggacaggacg cagggacttc atcgcgggtc
      721 tcgttcgcat cagaggagat ggcaagcggc caggtgcatg tcggtggaga ccatgcggcg
      781 cagctcgggc acgctctcgc ggcaacgcgg ctgagccagc gcgcagcggg tgtggaaggc
      841 gcagccggag ggcgcgtgca gcgggctggg cacgtccccc tccagcagat ggccggcacg
      901 gccgcggcgc ggcacggggt cggggaccgc ggccagcagc gcctgcgtgt aggggtgaag
      961 cggcgatgcg aacagcgccg cggcgggggc ggtctcgacg acgcggccca ggtacatcac
     1021 ggccacgcgg tcgcacagcc atgccaccac ggacaggtcg tgcgagacga acacgtagct
     1081 cagcgcgagc cgttcctgca ggtcgaccag caggttgagc acctgcgact ggatcgacat
     1141 gtcgagcgcg gacagcggct cgtcgagcac cacgaccttg ggctgcagcg cgagtgcccg
     1201 cgcgatgccc acgcgctggc gctgtccgcc cgagagttcg tggggccagc ggtcgaggtg
     1261 ctctggcgcg aggcccacca gcgccgccag ttcgcgcagg cgtgccgcgt cgaccggcaa
     1321 cccgtgcgcc tcgaacggca gcgcgagcgc ctggcgcacg cgcttgcgcg ggttcagcga
     1381 ggcgagcgga tcctggaaca ccatctgcac gtcgcgcgtc agcgcgcggc gctcctcgcg
     1441 cgacaacgtc gagacgtcgc ggccctcgac acgcagcgta cccgcgtcgg gctcgatgag
     1501 cccgaccagc gcgcggccca gcgtcgtctt gccgcagccc gactcgccga cgatgcccag
     1561 cacctcgccc gcggccacct gcacgtcgac gcgatcgacc gcgcgcagcc gcgcgacctc
     1621 gcgccgaagg agtccgccgc gcacggggaa tgcgacgctc agcccctcgg cctgcagcag
     1681 cggcgccgcg gtcatgcggg aacctcggtg ctgcgcattg cggcgcgagc acccttcgga
     1741 gcggcccggc gtgcgctcat gcgcgtccgc cttccgcgaa atggcaggcc gcggcatggt
     1801 cgccgaccgc gggcgtgacg cgcgcgagca ccggcgcctc cgtgcggcag cgcgcctgcg
     1861 cgcgcgggca gcgcgactgg tagcggcagc cgtcgatgcg cgcgccggcg gcgggcacgg
     1921 tgccggggat gacatccagc cgcgcgccgc cgcgccgcgt ctggccgggt cgcgggcgcg
     1981 cgcccagcag gccgatcgtg tacggatgcg cgggacgttc gagcacgtcg aacacgctgc
     2041 cctcctccac cacgcggccg ccatacatga cgacgacgcg gtcggcgact tcggccacga
     2101 cggccaggtc gtgcgtgacg aacacgatgc cggtgcccag ttcgcgctgc agctcgcgca
     2161 gcaggtgcag cacctgcgcc tggatcgtca cgtcgagcgc ggtggtgggc tcgtcggcga
     2221 tcagcagccc cggccggttg gcgagcgcca gcgcgatcat cacgcgctgc ttcatgccgc
     2281 cgctgagctg gtgcgggtag tcgtcgaccc ggcgttccgg gtcggggatc ttcacctggc
     2341 gcagcagcgc gatcgtcctg tcgcgcgcct cgcgccgcgt cgcggtgccg tggatggcga
     2401 acacctcggc gacctgcgcg ccgatcgtga gcaccgggtt cagcgccgtc atcggctcct
     2461 ggaacaccat cgccagcgcg cgcccgcgca ggccgcgccg ttcgcgcggc ggcagcgcca
     2521 gcaggtcgac gccgcgcagc cggatcgcac cggcctcgac ccgcgccgcc tcgggcagca
     2581 gccccatcac cgacagcgcg gtcacgctct tgccgcaacc cgactcgccc gccacgcaca
     2641 ccgtctgccc gcgctccacg cgcaggctga cgccgtcgac cgcgcgcacg gggccgcgcg
     2701 gcgtccggaa cgaggtgacg aggccgtcga tctcgagcag tgggggctcg ctcatggcgt
     2761 tgcggcgctt ggctgtggag gcaattgcgg gaggccgcgg gatgatgcca cagcgtagtt
     2821 ggcgggcggc ggctgcgcgg ctgtgcggtt gctaccgtta cggtcgctgt cgacgctgtt
     2881 gcagctgttg tcgcttcgcg cgtgactgcg tcgcgcgggc gacaggccgg gccggggctc
     2941 cgctctccgg cttgccgtgc gtcggaatcc ggcctggacg gccggattca gcgggttccg
     3001 tggcttcgac ttcttcctcg cgctcgccac cggagcgcgt tcaacgactt gattggcgga
     3061 ccgggggctt ccacccgcgt cgcgccggtc gcgcctgcga tcgaagcccc ggcccaccct
     3121 gccgcgccag cgtgtcagcc gttggagaaa aacattcgtg atttttggaa ggggttttgg
     3181 catcgacggg ccatcgaggc cgcgtcgatg cccgcggcca gcgctctggc ggggtgccgg
     3241 gcttgacgcg gatcgcgggc caccgtgtca gcgctccttc tcgtgtccga cgaccacgtc
     3301 accttcggcg ccgtgaactc ggagtacgct gcgcggccgt gcggcagcgc agccgcacgg
     3361 cgtcgccgag ttcaacgacg ctgtagcgga agcggaaggc gacagcctga ctggcggcgc
     3421 caacggttgc ggccccgtat cgactcgtcc ggtccgttga tttgactgcg ttgtcagggg
     3481 tgcaaggctg ggtgcgaccc gcagccattc agcgccgtcc ggatgagcat ggagggcgaa
     3541 gcgcagtccc gcggcgtgtc cgatgactgc agaaatagcg gaactttgca gcgaatatcg
     3601 ccgcatcgcc gcacccgtgc cgttcaatga tcgccctcag tggtcgaaag actttggacc
     3661 acgacacagg agaaccgatg atggtcgttc gcgcgctcct ttccatgctg ctgctggccc
     3721 ttgccagttg tgcgtccaag gacgctcagc cacctgctcc tgccggcatt ggcgccgcag
     3781 atccgtggat gcggcctgcc gcggtgcctt tccccgcgga caacgcgccg aatgcatcgc
     3841 gcgtcgcgct cgggcgcgat ctgttcttcg atacgcgtct gtcaggatca tccatgatca
     3901 gctgcgcgac ctgccacaac ccggcgctgg gatggtcgga cgggcagccc accgcggtcg
     3961 gcgacggggc caagcgcctg ggacgcaaga cacccaccgt cctgaacacg gcctatcagc
     4021 cgctgcagat gtgggacggc cgcatgaagt cgctcgaaga ccaggccctc ggcccgatcg
     4081 gttcgacggc agagatgaac atgccgctcg atcgcatggt cgaacatctt cgcgcgatac
     4141 cgggatacgc gcccatgttc gaacaggcat atccaggcga gggcgtcacc cagggcacgg
     4201 tggccaaggc gatcgccagc tttgaacgca cggtcgtctc gaccacctcg ccgttcgacc
     4261 gctggcgtcg cggcgacgaa caggccgtgg acgcatccgc caagcgcgga ttcgcgctgt
     4321 tccagggcaa ggcgcgctgc acgctctgcc accagggctt caacttcacc gacaacggct
     4381 atcacaacat cggcatccgc tcgctgtccg gcgccgaaga cgacgggcgg ttcgcgcacc
     4441 gaaagctgaa ggtgctcaag ggcgccttca agacgccgac gctgcgcgac gtggaactca
     4501 cggccccgta catgcacaac ggcatctacc gcacgttgga ggaggtcgtt gaccactacg
     4561 accgcggcgg cgatgtcaag gacaacctca gcccgaacat ccagccgctc ggactcacgg
     4621 ccgaggaaaa gcacgatctg gtggccttca tgaagtccct cacgggccag ccgatggtgg
     4681 tcgaggttcc tcacttgccg ccgggctgac ctcggccatc gatccacatt ccaggagtcc
     4741 agccatgatg aaagccctgc ttgccttgct ctcgtgcctc gccgtcctgt ctcccgcgtt
     4801 cgcggcccag gaggtggtcg tcgcgcagaa gaacaagtcc ttttcgcagc ccaggatcac
     4861 gatccacgcc ggcgacaagg tgacgttccg caatgacgac gccttcgtcc acaacgtctt
     4921 cagtctcacg gatgcgcaac ccttcgacct cggcacctac acccaaggac aggccaagtc
     4981 ggtgacgttc gccaaggcgg gcacctacga catcgagtgc gcgatccatc ccgagatgaa
     5041 gctcaccgtc gtcgtcgccc catgaagacg cgcgccgtcg cggcactggc agcgctgctg
     5101 gtcgctggag tcgcacgcgc cgcaggcact gacgaggcca tcgcgcgcgg aggcaagctg
     5161 ttcggtgacc gctgcgcgtt gtgccacggc agcaatgccg acggcaaggg gcagctcgcc
     5221 aagatgctgg atcccaagcc ggcaaacctg cgggcaagcc ttctggaccg cggcgcacgc
     5281 gaatcgatca tccgacatgg cggcgccgcg gtcgggcgtt cgccggccat gccgcgctgg
     5341 gaagtcgaat tcgacgagca gcaactggac gacgtgatcg cgtatgtcga ctcgatcgta
     5401 ccggcgcatt cggggaacac gccatgaagc ggtcgatgat cgcctggctg cttgcgctgt
     5461 tgccgctgct ggtggccgct gcacctgaag acggcgaacc gccattggcc ggcctgggcg
     5521 gacccatcga cctcgtcgac cagggcggcc atagcttcca tctcgcgcaa ctgggacggc
     5581 gccgcgcgct cgtcttcttc ggattcacgg gctgcggaac catgtgcccg acggcaatgc
     5641 tcaccgcgcg gcaggtgctc gcgcgcacac cggcggg
//
LOCUS       DATCRO010000453         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_623613, whole genome shotgun sequence.
ACCESSION   DATCRO010000453 DATCRO010000000
VERSION     DATCRO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623613"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..257)
                     /locus_tag="VIN75_12300"
     CDS             complement(63..257)
                     /locus_tag="VIN75_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY8974996.1"
                     /translation="MQSNEGNIDRAARITVGVGLCVAALVGALGPWAWIGVVPLATGF
                     VGFCPLYSLLGINTCSMDRH"
     gene            complement(320..2170)
                     /gene="ftsH"
                     /locus_tag="VIN75_12305"
     CDS             complement(320..2170)
                     /gene="ftsH"
                     /locus_tag="VIN75_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8974997.1"
                     /translation="MPPIDRRTLRRFAWFVLLAAAWLLLIVAGRGAGTAKAVSYDVFE
                     IALSQGRVERVQATDTLLIGHLWEKEGGYTDLTTVRVDPALAARLDRFKVPYEQVPDS
                     LVGRFIAWIAPTALLVALWYFMMRRTSGSGGLGGLMGVGRSNAKVHVENDTGVTFDDV
                     AGIDEAKAELEEVVQFLRHPQEFGRLGARIPRGVLLVGPPGTGKTMLAKAVAGQAQVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARQRAPAIVFIDELDALGRARGLGGGWTGND
                     EKEQTLNQLLTEMDGFDTSAGVVMLAATNRPAILDPALLRAGRFDRQVLIDRPDRTGR
                     NAILAVHARKVRLDPATRLDDVAALTVGFSGADLANLVNEAALAATRRKADFVAREDF
                     VVAIERIVAGLEKRHRVLNAKERERVAWHEMGHALVAIAQHGGDVVRKVSIVPRGVGA
                     LGYTLRSPLEDRYLMSRGELEARLAVLMAGRAAEVLVFGDASTGAADDLAKATDIAAD
                     MVSRQGMDAALGNVAYERQAMPPFAFGDGGPHAANGASEDTRRRIDEAVRRLLGRAFD
                     DATRLLRTHRADLDRCARALLERETLDEDAILVLAPGLRDLRTDEAVETT"
     gene            complement(2255..>3417)
                     /locus_tag="VIN75_12310"
     CDS             complement(2255..>3417)
                     /locus_tag="VIN75_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8974998.1"
                     /translation="RHPSIASARAASQQAHAVTVVARAAVQPRLSVGFVAARGNDLDA
                     STHVSSERSIGPNLAWSPDINGAVARRVERAKALEDEQRANESAAALALATEVVVDSI
                     AMAAADERRQAMSAIVAIDESLAEMARRAWRAGRSAQVDVFAAEQRVASDRVALASIE
                     HDASATRQALALLTGRLPGEWLPASIALDDFALPRDLPLRLPSELVRQRPDLLAAEAR
                     LHAASASIGIASSGLYPNVTLDSGWTLAASPAAVVGGGQGVWSLAATLLAPVFDGQDR
                     QARKQAAVAAYAGQLGEYRAAVLQAFGEVAAAMQALERDAITAPAAGLAWEAADRAAN
                     AACLSHRAGRSALSECLLARREEHLGRITRSEAAAGRLADSARWFAAMGAAD"
BASE COUNT          486 a         1277 c         1189 g          465 t
ORIGIN      
        1 cccttcatcg gccaggaagc ggcgaggcgg cgagtccgcc cgccgcttcg ccgtccgtgg
       61 cttcagtgcc ggtccatcga acaggtgttg atgcccaaca gcgaatagag cgggcagaag
      121 cccacgaagc cggtggccag cggcaccacg ccgatccagg cccagggccc cagggcgccg
      181 accagcgccg ccacgcacag gcccacgccc acggtgatgc gggcggcccg gtcgatgttg
      241 ccttcgttcg attgcatgct gatctccttt gcgaaagatc cagcatggcg ccggacgacc
      301 ccgccgcgat tgctcgcgct caagtcgtct cgacggcctc gtccgtcctc aggtcgcgca
      361 agccgggcgc caggaccagg atcgcgtctt cgtccagcgt ctcccgttcg agcagcgccc
      421 gggcacagcg atccagatcc gcgcgatggg ttcgaagcag ccgggtcgcg tcgtcgaagg
      481 cccggcccag caggcggcgg acggcctcgt cgatgcggcg acgcgtgtcc tccgatgcgc
      541 cgttggcggc atggggccca ccgtcgccga aagcgaatgg cggcatcgcc tgccgctcgt
      601 aggcgacgtt gccgagcgcg gcatccatgc cttgccggct gaccatgtcg gcggcgatgt
      661 ccgtcgcctt ggccaggtcg tcggcggcgc cggtggacgc atcgccgaag acgagcacct
      721 cggccgcgcg ccccgccatc agcacggcca ggcgcgcctc gagttcgccg cgcgacatga
      781 ggtagcggtc ctcgagcggg ctgcgcagcg tgtagcccaa cgcgcccacg ccgcgcggga
      841 cgatcgacac cttgcgcacg acgtcgccgc cgtgctgcgc gatggccacc agtgcgtgtc
      901 ccatctcgtg ccaggcgacc cgctcgcgtt ccttcgcgtt gaggacgcgg tggcgcttct
      961 ccagtccggc cacgatgcgt tcgatcgcga cgacgaagtc ctcccgcgcc acgaaatcgg
     1021 ccttgcggcg ggtcgccgcc aaggccgcct cgttgacgag gttggcaagg tcggcgccgc
     1081 tgaatcccac ggtcagcgcc gcgacgtcgt ccaggcgggt cgccgggtcc agccgcacct
     1141 tgcgcgcgtg gacggcgagg atcgcgttgc gccccgtgcg gtccggacgg tcgatgagca
     1201 cctgccggtc gaagcggccg gcgcgcagca acgcagggtc gaggatcgcc ggccggttgg
     1261 tcgcggccag catcaccacg ccggcggacg tgtcgaagcc gtccatctcg gtcaggagct
     1321 ggttgagcgt ctgctccttc tcgtcgttgc ccgtccagcc gccgcccagg ccgcgcgccc
     1381 ggcccagggc gtcgagttcg tcgatgaaga cgatggcggg cgcccgctga cgcgcctgct
     1441 cgaacaggtc gcgcacccgc gcggcgccca cgccgacgaa catctcgacg aactcgctgc
     1501 cggagatcga gaagaacggc acctgcgcct gcccggccac ggccttggcc agcatcgtct
     1561 tgcccgtgcc cggcgggccg accagcagca cgccgcgcgg gatgcgcgcc cccaggcgcc
     1621 cgaactcctg cggatggcgc aggaactgca ccacctcctc gagctccgcc ttggcctcgt
     1681 cgatgcccgc cacgtcgtcg aaggtgacgc cggtgtcgtt ctcgacatgc accttggcgt
     1741 tgctgcgccc gacgcccatc aacccgccga gaccgccgct gcctgaggtc cggcgcatca
     1801 tgaagtacca cagcgcgacc agcagcgccg tgggcgcgat ccaggcgatg aagcggccga
     1861 ccaggctgtc gggcacctgc tcgtaaggca ccttgaagcg gtcgaggcgg gcggccagcg
     1921 cgggatcgac gcgcacggtg gtcaagtcgg tgtagccacc ttccttttcc cacaggtgcc
     1981 cgatcaacag ggtgtccgtg gcctggaccc gctccacccg cccctgggac agcgcgatct
     2041 cgaagacgtc gtacgagaca gccttggccg tcccggcacc gcggcccgcg acgatgagca
     2101 gcagccaggc ggccgccagc aggacgaacc aggcgaacct gcggagtgtc ctgcgatcga
     2161 tcggcggcat cgggcggtcc ggcatggggg ccaacatacg cgacacgacg agccatccgc
     2221 ttgcgccttc gcaatgtggc gtgcgaccgg tgcgtcaatc ggcggcgccc atcgccgcga
     2281 accagcgtgc actgtcggcg aggcgtccgg cggccgcctc gctgcgggtg atgcgtccca
     2341 gatgctcctc gcgccgcgcc agcaggcatt ccgaaagcgc ggaccggccc gcccgatggc
     2401 tgaggcaggc ggcgttcgcg gcgcgatccg ccgcctccca ggccagtccg gcggccggcg
     2461 ccgtgatggc gtcccgctcg agggcctgca tggcagccgc gacctcgccg aacgcctgga
     2521 gcacggccgc acggtactct ccgagctggc cggcatacgc cgccaccgcc gcctgcttgc
     2581 gcgcctggcg gtcctgtccg tcgaagaccg gtgcgagcag cgtcgcggcc aggctccaga
     2641 cgccctgccc cccgccgacc accgcggcgg gcgatgccgc cagcgtccag ccgctgtcca
     2701 gcgtgacgtt cggatagagg ccggacgagg cgatcccgat ggacgcgctc gccgcatgca
     2761 ggcgggcctc cgcggcgagc aggtccgggc gctggcgcac cagttcggaa ggcaggcgca
     2821 gcggcaggtc gcgcggcagg gcgaagtcat ccagcgcgat ggacgccggc agccattcgc
     2881 cgggaaggcg ccccgtcagc agcgcgagcg cctggcgggt cgccgacgcg tcatgctcga
     2941 tcgacgcgag ggcgacgcgg tcgctggcga cgcgctgctc ggcggcgaac acgtccacct
     3001 gcgcgctcct gccggcccgc catgcgcgac gcgccatttc tgccagcgac tcgtcgatcg
     3061 ccacgatcgc cgacatcgcc tggcgtcgtt cgtccgcggc ggccatggcg atggaatcca
     3121 ccacgacctc cgtcgccagg gccagcgccg ctgcactctc gttggcgcgc tgttcgtctt
     3181 ccaacgcctt cgcgcgctcg acccggcgtg ccacggcacc gttgatgtcg ggcgaccagg
     3241 ccaggttggg gccgatcgac cgctcgctgg acacatgcgt gctggcgtcc agatcgttgc
     3301 cgcgtgccgc cacgaaaccg acggacagcc gaggctggac ggccgcgcgg gcgacgacag
     3361 tgaccgcatg cgcctgctgg ctcgccgcgc gcgcactggc aatcgacgga tggcgcc
//
LOCUS       DATCRO010000454         9734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_151275, whole genome shotgun sequence.
ACCESSION   DATCRO010000454 DATCRO010000000
VERSION     DATCRO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151275"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VIN75_12315"
     CDS             <1..327
                     /locus_tag="VIN75_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY8974999.1"
                     /translation="PLVAALVATAMPAISMADPYDHDWHAHHEWNHPGYRHDDYRGVG
                     PDHGWHRGDRLPNEYWGHRHEVADWHARNLAPPPRGYHWVEVNGDYVLAAIATGIILD
                     TLLTPQ"
     gene            complement(334..708)
                     /locus_tag="VIN75_12320"
     CDS             complement(334..708)
                     /locus_tag="VIN75_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8975000.1"
                     /translation="MPSPQTLERFIAMVESNDHAQACELFYTEHSTMQENQGTPRVGR
                     DAHVANERQVMARAEAVTSTCVRPVLVNGDHVVIRWIFQFTWRDGSITRMEELAWQRW
                     EGERIAEETFFYDPAQRVPHRP"
     gene            complement(753..1160)
                     /locus_tag="VIN75_12325"
     CDS             complement(753..1160)
                     /locus_tag="VIN75_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8975001.1"
                     /translation="MIPVFDRIDHIHVYVANRNKAEQWYADVMGFSRVPELEAWAADG
                     PLTLGNSSGTVHIALFEAAPQPCRSVIALSVGAAEFVAWRQHLVDKLRQPVEPEDHQL
                     SWSLYFADPDGNPWEITSYQHAVVATLLKPVGT"
     gene            1427..3040
                     /locus_tag="VIN75_12330"
     CDS             1427..3040
                     /locus_tag="VIN75_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8975002.1"
                     /translation="MNTNVGSMPGGLAASSRPRLDHKPGAATWIVFAALLLAGLGYTA
                     WSIATDYAGSGEQATAIWPFVLLGLALLIALGFEFVNGFHDTANAVATVIYTNSLPPQ
                     VAVAWSGLFNFLGVLVSSGAVAFGIISLLPVELILQVGSSAGFAMVFALLIAAIVWNL
                     GTWALGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWSQAVKIGWSLLLSPLIGF
                     IASALLLLAVRALVRNKALYEAPKGSTPPPWWIRGLLVLTCTGVSFSHGSNDGQKGMG
                     LIMLILVGTVPISYALNRAMPVDQTAQFAIVAHATHDSLTAAAPGAAPADARATLSHY
                     VRTKVVESDTLPALAAIAGSIGDQVQQHDTLAKVPAATVANVRNDMYLASETIRLVDK
                     DSRVRLDAGMHDKLMAFKKDIDGATKFIPLWVKVVVAIALGLGTMVGWKRIVVTVGEK
                     IGKSHLTYAQGGSAELVAMFTIGLADNFGLPVSTTHVLSSGVAGTMAANRSGLQWATI
                     RNLAMAWVLTLPAAVLLSGCLYWLLAKLF"
     gene            3054..3971
                     /gene="ppk2"
                     /locus_tag="VIN75_12335"
     CDS             3054..3971
                     /gene="ppk2"
                     /locus_tag="VIN75_12335"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY8975003.1"
                     /translation="MNALFDQDAMRRRIERELVDGYDEEIEMEMEDRLVADPLDASGE
                     DAAERVRYFRELFRLQGELVKLQDWVSSTRQKVVILFEGRDAAGKGGVIKRITQRLNP
                     RVCRTVALPAPNDRERTQWYFQRYAAHLPAAGEMVLFDRSWYNRAGVERVMGFCTDDE
                     YEEFFRSVPAFEGMLQRSGIKLIKYWFSITDDEQELRFLSRIHDPLKQWKLSPMDLES
                     RRRWEEYTKAKEVMLERTHSAESPWWVVQAVDKKLARLNCIHHLLGQFPYTEVQHPPV
                     QLPERMRHEHYSRQAVPEEMMVPDHYGEK"
     gene            complement(4054..4737)
                     /locus_tag="VIN75_12340"
     CDS             complement(4054..4737)
                     /locus_tag="VIN75_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975004.1"
                     /translation="MTRPPSMSTRAKAAAAAFAPAGLAGEVDAVKAAAAVARDQPALV
                     AAPVVWLRDHPGDMAPPAAADVAALRAFLLALEGEAAGTSDPPDPAAADAPRSALDAP
                     LCALCRGRCCRFGLDGRAFLEPQHLRAWLARHPGAAWADAVGHWLGFIGAEHLRGSCL
                     FHGRAGCTLPRERRSDVCNQFACDALEQARDVAAADPRAVVVAGIAASRELRGVAEVS
                     RAGVRPLLP"
     gene            4846..5919
                     /locus_tag="VIN75_12345"
     CDS             4846..5919
                     /locus_tag="VIN75_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY8975005.1"
                     /translation="MLFDPIRIRNLQLSNRTVMSPMTRSRAVEANTPNALMAEYYAQR
                     ASAGLIVTEGTSPSPDGLGYARIPGLFDARHVAGWKLVTDAVHAAGGRIVVQLMHTGR
                     VGHLANLPAGAQVLGPTAEALPGQMYTDSQGMQPHTPPRAMTEADIARAVDEFAQSAR
                     LAVEAGFDGVELHAANGYLIEQFLNPNVNTRTDRYGGTIAGRNRFALEVARAAAAAIG
                     GDKVGIRLSPYGVFNGTGPFADVEAQYLALVTELSTLGLLYLHVLDHSAMGAPAVPAA
                     IKTTLRRAFNGPFIAAGGFDAASAEQVLARGEADLVAFGRPFLANPDLVARMKAAAPM
                     NDVDMDTFYTPGAKGYTDYPALA"
     gene            complement(5920..6978)
                     /locus_tag="VIN75_12350"
     CDS             complement(5920..6978)
                     /locus_tag="VIN75_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8975006.1"
                     /translation="MSQQERFAPAAGTPPPALGDIRYGSDPNGLICGYLFAGGGPGVA
                     IDLAGALAWLADEASAAQGDFVWLHFNLTDAGAERWIAANLAVAPEFFEALHEGSRST
                     RIEDARDTLVAVVNDVSFEFSFDPSQIETLWANVGPRMAITARVHALRSIDRLRHAVR
                     QGITFRSSLLFLDRLLRDQGDVLVRIVRQATLQVDEVEDKLLVGRLGDKREDLGKLRR
                     VLVRLQRLLAPEPGALFRLLRQPPPWTGETDLDALRQATEEFSLVLRDLDSLQDRIKL
                     LQDEIAAQVGEHTNSSVFTLTVVTVLALPINLVAGLLGMNVGGIPLAQDPHGFLWVAA
                     LVGGFTALAGWIAFRRRD"
     gene            7095..8288
                     /locus_tag="VIN75_12355"
     CDS             7095..8288
                     /locus_tag="VIN75_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8975007.1"
                     /translation="MNVPLRATAALLAASLTLPALGADAAAPPASPASTPAAARCAAP
                     TADKAANRACALALAKAADADRAANRNDAALAALARADQFLPEDLRFVTARAGLKLKL
                     ANQLTPAGIEAAAKAAPDDVGLAMMHAELASAKKDPEAALADIDRVIAARPGSPQAWE
                     MRASINVARPDFAAARTDVEHALALDPKSPVVLRLRGVLRNNAGDHAGALADYEAAHA
                     LAPRPDDPFVIGSTQFLMRQFGDAAASLARRPPPAPDGLYWRIWRYLALARLNGVERA
                     GGALGPGTPPGPGLPWPAPVIDFMRGSIDAATLLADAKRSQDARDLSQVCEAHFYLGE
                     DALLGQRADAVEQFRAAAKECPVNFHEYEGATAELRAMGVPLVAPGPTVAAVQAAASA
                     ASQPR"
     gene            complement(8418..>9734)
                     /locus_tag="VIN75_12360"
     CDS             complement(8418..>9734)
                     /locus_tag="VIN75_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY8975008.1"
                     /translation="LLGTTAQSWPAKGEIDVMEQGHRQASWSAAGAPSPNNFVGSNVI
                     TYQQAACVPGNESCAASTSWQTKSWYVAPTSLANRFVTYRLYWTDTQMRFTIVDNGVE
                     HDMYDAPLPVNSPSLQAPFYLLLNLAVGGNFTDAATPGQVTAPLPGTMYVDYVRVYQL
                     DGKGTVKLGNQIVPETGKFGVFTDNTPVTHKLVAGTSSDIYMWNTASMAAGNIAPFEG
                     SNVIAWAYTAPGQWFGGGIQSRDIKDMTNFRNGKMSFRIKIPANVSFKVGIGDTYTNQ
                     NWISFPANAPTYGLVRNGDWAQATIPVSDLAGPKVALQSLHDIFMIANDSPVPGATFQ
                     FAVDDIVWDSGTATPPPPPSGPTSITQTSASTLQLNTSTGSWADVHYTVNNGGQMNVR
                     MTLSGSTNTYVISGLKTGDVVKYSFTYWDTTKNYAIDTAQQSYTMQ"
BASE COUNT         1424 a         3502 c         3392 g         1416 t
ORIGIN      
        1 cccctcgtcg ccgcgctggt ggcgaccgcc atgccggcga tctcgatggc cgacccgtac
       61 gaccacgact ggcatgccca ccacgagtgg aaccatccgg gctaccgcca tgacgactac
      121 cgcggcgtcg gccccgacca cggctggcat cgcggcgacc gcctgcccaa cgagtactgg
      181 ggccatcgcc acgaagtggc cgactggcat gcccgcaacc tcgccccgcc gccgcgcggc
      241 taccactggg tcgaggtcaa cggcgactac gtgctggcgg ccatcgccac cggcatcatc
      301 ctggacacgc tgctgacccc gcagtgacga ccctcacggc cggtgcggca cccgctgcgc
      361 cggatcgtag aagaacgtct cctcggcgat ccgctcgccc tcccagcgct gccacgcgag
      421 ttcctccatg cgcgtgatgc tgccgtcgcg ccaggtgaac tggaagatcc agcggatgac
      481 gacatggtcg ccattgacca gcaccggccg cacgcaggtg gacgtgacgg cctcggcacg
      541 cgccatcacc tggcgctcgt tggcgacgtg cgcgtcgcgc ccgacgcgcg gcgtgccctg
      601 gttctcctgc atcgtcgagt gctcggtgta gaacagctcg caggcctgtg cgtggtcgtt
      661 gctctcgacc atcgcgatga atcgctcgag ggtttgcggt gacggcatcg tggcgctcct
      721 gtccacgcag tatcaccgcc gtgccgaagt cctcaggttc cgacgggctt gagcagggtc
      781 gccacgaccg cgtgctgata gctcgtgatc tcccacgggt tgccgtcggg atcggcgaag
      841 tacagcgacc acgagagctg gtggtcctcg ggctcgacgg gttgccggag cttgtcgacc
      901 aggtgctggc gccacgcgac gaactccgcg gcgcccaccg acagcgcgat cacgctgcgg
      961 cagggttgcg gtgcagcctc gaacagcgcg atgtgcacgg tgcccgacga attgcccagc
     1021 gtgagcgggc cgtccgcggc ccaggcctcc agctcgggca cgcgcgagaa gcccatcacg
     1081 tcggcatacc actgctccgc cttgttgcgg ttggcgacgt agacatggat gtggtcgatg
     1141 cggtcgaaga cgggaatcat ggcgtctcca gatgaggagt gcgaggacgg gcggcaattg
     1201 tcgcgcccgt tccgatcgtc cctgccatcg atccggggtc gacccggccg gcgcgcgacg
     1261 cgccacaggg cgtgtgcggc cccgccgatc ggcaggcgac cttccccgcg agcctcacgc
     1321 atcgcgcgcc cgcctttgcg cgcgagcgcc tgtcaccccg ttgtcatgcg accgcccgac
     1381 gatcccccgg ttgtcgcacg tcaagtcaga aagagggatc actgccatga acaccaatgt
     1441 cggctcgatg cccggcgggc tggccgcgtc cagccggccg cgcctggacc acaagccagg
     1501 cgcggccacg tggatcgtgt tcgccgccct gctgctggcg ggcctgggct acacggcctg
     1561 gagcatcgcc accgactatg ccggctccgg cgagcaggcc accgcgatct ggccgttcgt
     1621 gctgctgggc ctggcgctgc tgatcgcgct tggcttcgag ttcgtcaacg gcttccacga
     1681 caccgccaac gcggtcgcga cggtgatcta caccaactcg ctgccgccgc aggtggccgt
     1741 cgcgtggtcg ggcctgttca acttcctggg cgtactcgtg tccagcggcg cggtggcctt
     1801 cggcatcatc tcgctgctgc cggtggagct gatcctgcag gtgggttcgt cggcgggctt
     1861 cgcgatggtg ttcgcgctgc tgatcgcggc catcgtgtgg aacctgggca cctgggcgct
     1921 gggcctgccg gcgtcgtcgt cgcacacgct gatcggctcc atcatcggcg tgggcgtggc
     1981 caacgcgctg atgcacggcc gcgacggcac cagcggcgtg gactggtcgc aggccgtcaa
     2041 gatcggctgg tcgctgctgc tgtcgccgct gatcggcttc atcgcatcgg cgctgctgct
     2101 gctggcggtg cgcgcgctgg tcaggaacaa ggcgctgtac gaggcgccca agggctcgac
     2161 gccgccgccg tggtggatcc gcggactgct ggtgctgacc tgcaccggcg tgtcgttctc
     2221 gcacggctcc aacgacggcc agaagggcat gggcctgatc atgctgatcc tggtgggcac
     2281 ggtgccgatc tcgtacgcgc tgaaccgggc gatgcccgtc gaccagaccg cgcagttcgc
     2341 gatcgtcgcg catgcgacgc acgattcgct gaccgcggct gcgccgggcg ccgcgcccgc
     2401 ggacgcgcgc gcgacgctct cgcactatgt ccgcaccaag gtggtcgagt ccgacaccct
     2461 gcccgcgctg gccgccatcg ccggctcgat cggcgaccag gtgcagcagc acgacacgct
     2521 tgccaaggtg cccgccgcga ccgtggccaa cgtgcgcaac gacatgtacc tggcctccga
     2581 gacgatccgc ctcgtcgaca aggacagccg tgtcaggctc gacgccggca tgcacgacaa
     2641 gctgatggcg ttcaagaagg acatcgacgg cgcgaccaag ttcatcccgc tgtgggtgaa
     2701 ggtggtcgtg gccatcgcgc tgggcctggg cacgatggtg ggctggaagc gcatcgtcgt
     2761 gacggtcggc gagaagatcg gcaagtcgca cctgacctac gcacagggcg gctcggccga
     2821 actggtggcg atgttcacga tcggcctggc cgacaacttc ggcctgccgg tgtccaccac
     2881 gcacgtgctg tcgtccggcg tggccggcac gatggccgcg aaccggtcgg gcctgcagtg
     2941 ggccacgatc cgcaacctcg cgatggcctg ggtgctgacg ctgccggcgg cggtgctgct
     3001 gtccggctgc ctgtactggc tgctggccaa gctgttctga acggagggaa cgcatgaacg
     3061 cactgttcga ccaggacgcg atgcgccgcc gcatcgagcg cgagctcgtc gacggctacg
     3121 acgaagagat cgagatggag atggaggacc gcctcgtcgc cgacccgctg gacgccagcg
     3181 gcgaggacgc ggccgagcgc gtgcgctact tccgcgagct gttccgcctg cagggcgagc
     3241 tcgtgaagct gcaggactgg gtgtcgagca cgcgccagaa ggtggtcatc ctgttcgagg
     3301 gccgcgacgc ggccggcaag ggcggcgtca tcaagcgcat cacgcagcgc ctgaacccgc
     3361 gcgtgtgccg caccgtcgcc ctgcccgcgc ccaacgaccg cgagcgcacg cagtggtact
     3421 tccagcgcta cgccgcgcac ctgcccgccg ccggcgagat ggtgctgttc gaccgcagct
     3481 ggtacaaccg cgccggcgtc gagcgcgtga tgggcttttg caccgacgac gaatacgagg
     3541 agttcttccg ctccgtgccc gcgttcgaag gcatgctgca gcgctcgggc atcaagctga
     3601 tcaagtactg gttctcgatc accgacgacg agcaggagct gcgcttcctc agccgcatcc
     3661 acgacccgct caagcagtgg aagctgagcc cgatggacct cgagagccgg cgccgctggg
     3721 aggagtacac caaggccaag gaagtgatgc tggaacgcac gcattcggcc gagtcgccgt
     3781 ggtgggtcgt gcaggccgtc gacaagaagc tggcgcgcct gaactgcatc catcacctgc
     3841 tcgggcagtt cccgtacacc gaggtccagc acccgcccgt gcagttgccc gagcgcatgc
     3901 gccatgagca ctactcgcgc caagccgtgc cggaagagat gatggtgccg gaccactacg
     3961 gcgagaaata agaaagaagc gacccttgtc gccctgcggg tcgcgcatgc ctgcggacgc
     4021 gcggccttct cgtgcggcgc tgccggcgct cgttcaggga agcagcggcc gcacgcccgc
     4081 gcgcgacacc tcggcgacac cgcgcagttc acgcgacgcc gcgatgcccg ccacgacgac
     4141 cgcccgcgga tccgccgccg ccacgtcgcg cgcctgctcc agcgcgtcgc acgcgaactg
     4201 gttgcacacg tcggagcggc gctcgcgtgg cagcgtgcag cctgcgcgcc catggaacag
     4261 gcacgagccg cgcaggtgct ccgcgccgat gaagcccagc cagtggccga ccgcgtcggc
     4321 ccaggccgcg cccggatggc gcgcgagcca ggcgcgcagg tgctgcggct ccaggaaggc
     4381 ccggccgtcg aggccgaagc ggcagcagcg gccgcggcac agcgcgcaca gcggagcgtc
     4441 gagcgccgag cgcggtgcgt cggcggccgc cgggtccggt gggtccgatg tgcccgccgc
     4501 ctcgccctcg agcgccagca ggaaggcgcg cagcgccgcg acgtcggccg ccgccggcgg
     4561 cgccatgtcg ccggggtgat ctcgcaacca cacgacgggc gccgccacca gcgccggctg
     4621 gtcgcgggcc acggcggcgg cggccttgac cgcatcgact tcgcccgcga ggccggccgg
     4681 cgcgaaggcc gcggcggcgg ccttcgcgcg ggtggacatc gacggcgggc gggtcatggg
     4741 gccatcctag tcctgccagt ttccgaaagg catgacaggc catgaggaca acgcgtcccg
     4801 ctcctatcat cggcgcaaat ctcaactctt ccaccaagac ccgccatgct gttcgacccg
     4861 atccgcatcc gcaacctgca actgtccaac cgcacggtga tgtcgccgat gacgcgcagc
     4921 cgcgccgtcg aggccaacac gcccaacgcg ttgatggccg agtactacgc gcagcgcgcg
     4981 tccgccggcc tgatcgtcac cgaaggcacg tcgccgtcgc ccgacggcct gggctacgcc
     5041 cgcatccccg gcctgttcga cgcgcgccac gtggccgggt ggaagctggt caccgacgcg
     5101 gtgcacgccg ccggcggccg catcgtggtg cagctgatgc acaccggtcg cgtcggccat
     5161 ctcgccaacc tgccggccgg cgcgcaagtg ctgggcccga ccgccgaggc gctgcccggc
     5221 cagatgtaca ccgacagcca gggcatgcag ccgcacaccc cgccgcgcgc gatgaccgag
     5281 gccgacatcg cccgcgcggt cgacgagttc gcgcagtccg cgcgcctggc cgtcgaggcc
     5341 ggcttcgacg gcgtcgagct gcacgcggcc aacggctacc tgatcgagca gttcctcaac
     5401 ccgaacgtca acacgcgcac cgaccgctac ggcggcacga tcgccggccg caaccgcttc
     5461 gcgctggagg tggcgcgcgc cgcggccgcg gccatcggcg gcgacaaggt gggcatccgc
     5521 ctgtcgccct acggcgtgtt caacggcacc ggcccgttcg ccgatgtcga ggcgcagtac
     5581 ctcgcgctgg tcaccgagct gtcgacgctg ggcctgctct acctgcacgt gctcgatcac
     5641 tcggcgatgg gtgccccggc cgtgccggcc gcgatcaaga ctaccctgcg gcgcgcgttc
     5701 aacggcccgt tcatcgccgc cggcggcttc gacgcggcga gcgcggagca ggtgctggcc
     5761 cggggcgagg ccgacctggt ggccttcggc cgcccgttcc tcgccaaccc cgacctcgtc
     5821 gcgcgcatga aggccgccgc gccgatgaac gacgtcgaca tggacacgtt ctacacgccg
     5881 ggcgccaagg gctacaccga ctatccggca ctggcctgat caatcccggc ggcggaaggc
     5941 gatccagccg gccagcgcgg tgaagccgcc caccagggcg gccacccaca ggaagccgtg
     6001 cgggtcctgc gcgagcggga tgccgccgac gttcatgccc agcaggcccg ccacgaggtt
     6061 gatcggcagc gcgagcacgg tcacgaccgt cagcgtgaac acgctggagt tcgtgtgctc
     6121 gcccacctgc gcggcgatct cgtcctgcag cagcttgatg cggtcctgca gcgaatcgag
     6181 gtcgcgcagc accagcgaga actcctcggt ggcctggcgc agcgcgtcga ggtccgtctc
     6241 gcccgtccac ggcggcggct ggcgcagcag gcggaacagc gcgccgggct cgggcgccag
     6301 caggcgctgc agacgcacca gcacgcggcg cagcttgccc aggtcctcgc gcttgtcgcc
     6361 caggcgcccg accagcagct tgtcctccac ctcgtcgacc tgcagcgtgg cctggcgcac
     6421 gatgcgcacc agcacgtcgc cctggtcgcg cagcaggcga tcgaggaaca gcagcgacga
     6481 gcggaacgtg atgccctggc gcaccgcgtg gcgcaggcgg tcgatcgagc gcagcgcgtg
     6541 cacgcgcgcc gtgatcgcca tgcgcggacc gacgttggcc cacagcgtct cgatctgcga
     6601 cgggtcgaac gagaactcga aggacacgtc gttgaccacg gccaccagcg tgtcgcgcgc
     6661 gtcctcgatg cgcgtggagc gcgagccctc gtgcagcgcc tcgaagaact ccggcgcgac
     6721 ggccaggttc gccgcgatcc agcgctcggc gcccgcgtcg gtgaggttga agtgcagcca
     6781 gacgaagtcg ccctgcgcgg ccgacgcttc atcggcgagc caggccagcg cgccggccag
     6841 gtcgatggcc acgcccggcc cgccgcccgc gaacaggtag ccgcagatca ggccgttggg
     6901 gtcggagccg tagcggatgt cgccgagcgc cggcggcggc gtacctgcgg ctggcgcgaa
     6961 tcgttcttgc tggctcattg ccccggacct cccatgacga ccggcggatg gtagcgcgcg
     7021 accgttcggg cgcttttaca ggcccatggc aagatcgccg gcatcggcac ccgctgcctt
     7081 ctcgaccgta tcgcatgaac gtcccgctcc gcgcgaccgc cgccctgctc gcggcctcgc
     7141 tgaccctgcc cgccctgggt gccgacgcgg ccgcgccgcc cgcctccccc gcgtccacgc
     7201 ccgccgccgc caggtgcgcg gcgcccaccg ccgacaaggc cgccaaccgc gcctgcgcgc
     7261 tggcactggc caaggccgcc gacgccgacc gcgcggccaa ccgcaacgac gccgcgctgg
     7321 ccgcgctggc gcgcgccgac cagttcctgc ccgaggacct gcgcttcgtc accgcgcgcg
     7381 ccggtttgaa gctcaagctc gccaaccagc tcacgcccgc cggcatcgag gccgcggcca
     7441 aggcggcgcc agacgatgtg ggcctggcga tgatgcacgc cgaactggcg tcggccaaga
     7501 aggaccccga ggcggcgctg gccgacatcg atcgcgtgat cgcggcacgg ccgggttcgc
     7561 cccaggcctg ggagatgcgc gcctcgatca acgtggccag gcccgacttc gccgccgcgc
     7621 ggaccgacgt cgagcacgcg ctggccctcg atccgaagtc gccggtcgtg ctgcggctgc
     7681 gcggcgtgct gcgcaacaac gcgggcgacc atgccggcgc gctggccgac tacgaggccg
     7741 cgcacgccct cgcgccgcgg ccggacgatc cgttcgtcat cggctcgacg cagttcctca
     7801 tgcgccagtt cggcgacgcg gcggcgtcgc tggcccgtcg tccgccgccg gcgcccgacg
     7861 gcctgtactg gcgcatctgg cgctacctcg cgctcgcgcg cctgaacggc gtggaacgcg
     7921 ccggcggcgc gctgggcccc ggcaccccgc ccggccccgg cctgccatgg ccggcaccgg
     7981 tgatcgactt catgcgcggc tcgatcgacg ccgccacgct gctggccgac gcgaagcgct
     8041 cgcaggacgc gcgcgacctg tcgcaggtct gcgaggccca cttctatctg ggcgaggacg
     8101 ccctcctggg ccagcgcgcc gacgccgtcg aacagttccg cgccgcggcc aaggagtgcc
     8161 cggtcaactt ccacgagtac gaaggcgcga cggccgaact gcgcgcgatg ggcgtgccgc
     8221 tcgtcgcgcc cgggccgacc gtggcggcgg tgcaggccgc ggcgtccgcg gcgtcgcagc
     8281 caaggtaagc ctcgcgcgcc ggcgcccgcc gggttcaggc ggcgccggaa catcggggat
     8341 gcggccaacg agaaaggccc gcgcgaggcg ggcctggaca catcgctggc ggtgccgacg
     8401 ggcggtccgc gcccgcgtca ctgcatcgtg tagctttgct gcgcggtgtc gatcgcgtag
     8461 ttcttcgtgg tgtcccagta cgtgaagctg tacttgacca cgtcacccgt cttcaggccg
     8521 ctgatcacgt aggtgttggt gctgccgctc agggtcatgc gcacgttcat ctggccgccg
     8581 ttgttgacgg tgtagtgcac gtcggcccag ctgccggtcg atgtgttcag ctgcagcgtg
     8641 ctggccgagg tctgcgtgat ggacgtcggg ccgctgggcg gcggcggcgg cgtggccgtg
     8701 cccgaatccc agacgatgtc gtcgaccgcg aactggaacg tggcacccgg cacgggcgag
     8761 tcgttcgcga tcatgaagat gtcgtgcagg gattgcagcg ccaccttcgg accggccagg
     8821 tcggagaccg ggatcgtcgc ctgcgcccag tcgccgttgc gcaccaggcc gtaggtgggc
     8881 gcgttggccg ggaagctgat ccagttctgg ttggtgtagg tgtcgccgat gccgaccttg
     8941 aaggacacgt tcgccgggat cttgatccgg aacgacatct tgccgttgcg gaagttcgtc
     9001 atgtccttga tgtcgcgcga ctggatgccg ccgccgaacc actggccggg cgccgtgtag
     9061 gcccaggcga tgacgttgct gccttcgaac ggggcgatgt tgccggccgc catcgacgcg
     9121 gtgttccaca tgtagatgtc ggagctcgtc ccggccacca gcttgtgggt cacgggggtg
     9181 ttgtcggtga agacgccgaa cttgccggtc tcgggaacga tctggttgcc cagcttgacc
     9241 gtgcccttgc cgtccagctg atagacacgc acgtagtcga cgtacatcgt gcccggcagc
     9301 ggcgcggtca cctggccggg cgtcgcggcg tccgtgaagt tgccgccgac cgccaggttc
     9361 agcagcaggt agaacggggc ctgcagcgac ggcgaattga ccggcagcgg ggcgtcgtac
     9421 atgtcgtgct cgacgccgtt gtcgacgatc gtgaagcgca tctgcgtatc ggtccagtac
     9481 aggcgatacg tgacgaagcg gttggcgagc gacgtcggcg ccacgtacca gctcttggtc
     9541 tgccaggagg tcgatgcggc gcacgactcg ttgcccggga cgcaggccgc ctgctggtag
     9601 gtgatcacgt tcgagccgac gaaattgttc ggcgaggggg cgccggccgc cgaccaggag
     9661 gcctgcctgt ggccctgctc catcacgtcg atctcgccct tggcgggcca ggactgcgcg
     9721 gtcgtgccca ggag
//
LOCUS       DATCRO010000455        10448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_61053, whole genome shotgun sequence.
ACCESSION   DATCRO010000455 DATCRO010000000
VERSION     DATCRO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61053"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1103)
                     /locus_tag="VIN75_12365"
     CDS             complement(198..1103)
                     /locus_tag="VIN75_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8975009.1"
                     /translation="MKLADHAGHALARRAVLDPRIVVLDGDLADSDGAMHFAHAHPER
                     FFAAGIAEQNMVCVAAGMAECGWRPSVFSFAAFLCYRAYDQVRVSLSQASMPVTLVAS
                     HAGGLSGRNGKTHAAPNDVAIMLGLPHMDVWAPADQADVEFALAEVGRSQAPSYIRLP
                     RTPLGERDALPGEAAAFRFLRPARRTLLVSTGLATHWAMAAARELEALGIDVGVLHLA
                     RITPDLPAGLALAGVERSFVIEDHVPHGGLASHARSWAGLGEVTGITWPADFCGKSGS
                     DDEVRAEHGLDAPAIAARVARALRA"
     gene            complement(1090..1983)
                     /locus_tag="VIN75_12370"
     CDS             complement(1090..1983)
                     /locus_tag="VIN75_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8975010.1"
                     /translation="MNAPHPLHLEASDDVAVQTAPAGLGRAAEATLLRADIVRALHAA
                     GGGHYGGSLSVIDVLLTLYRGHVRVNPRAPRHPDRDRLLLSKGHAAVALYAVLRRLGF
                     FDDDLCTYADHASILEGHPDMKTVPGVDFSTGSLGQGLSVGCGMAIALRETGRRVFVV
                     LGDGECQEGQVWEAAMFASANAIANLTAVVDCNGLQEWGLRDRDGTALAPLTRAAQKW
                     RAFGWNVVECDGHDFAAIDAALGARHADPHAPTAVICHTRKGRGVPVAETRADEFHCA
                     AMTDEEHLATLRHLLPNHETR"
     gene            complement(1980..3812)
                     /locus_tag="VIN75_12375"
     CDS             complement(1980..3812)
                     /locus_tag="VIN75_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY8975011.1"
                     /translation="MSHASCLYALVAKTSAARDFLAAQLPAALPATFPQSRDAWWLEP
                     HAGLAIHAPVSDACGAFAAVGRVRRPESGWTPLGERTTDEAMLRQLCVAGPRALATLE
                     GGHAAVFWDGARQELSLLRDAFGQQTLFVREDVAFFVVCSELEPLLRDPRFHRALDVE
                     SAYHYLAFGSPVPGRTLARRVTRVRAGHLLRWTPGAPLIEQRHFTPLSHGSRKVAGDA
                     GRDEIARTLEDAILARSVPGRQALLLSGGIDSSYIGLTLAEHAGADQLDTYTVEFAAP
                     WPGNEGEYAAIVARQAGLRHALVPMSPADAARHLPKVLRAAEPCSAWASVTHHHLLDR
                     IVGDGHGLLHSGLGADEVFGGYSRFLDHYQLQREHETAFEFADRIDPFDAMLWQPAQA
                     GRRLFQGVPRFFSDEQLAGWLQQPCAGWDFAALLTAFYRDCRRIKPSAHLFELMVAHE
                     CQHRVPDLLFADFEPIARSRSLATVYPFLDTAVARQACGLGASERFSREGETWRNKRL
                     LREIASRRLPPEIMARKPVSYNAPILLWLKDPAFADIAMAYLDRCILTEIGLLSEEGL
                     RHIRDGVTALTPQSPPESRVVLDQAWAVITLAAWVEEWIGRTPA"
     gene            complement(3809..4876)
                     /locus_tag="VIN75_12380"
     CDS             complement(3809..4876)
                     /locus_tag="VIN75_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975012.1"
                     /translation="MFHKILVVGLGDLAERIVTRLATAGGVELVLAGRRPEVAAALAR
                     FASSLGDIAARGVPLDALDRAQVAELVARERPALIVQCAALVSPWALGGREDAAAVAL
                     RAAGFALQLCAQLPCVAAVTAAARDAGHAGPIVNCSYPDLTHPVLHRSGVAPDVGIGN
                     AGMVQALARAALGHAEREAPLRVVAHHFHVGAVARSDAALLGGLPLPRIFAGDREVDA
                     APLLFGHAPIALDRRLNELTAMHAATLIRAMLPGGAPLLTAAPGPLGLHGGWPVRIEH
                     GRVDLDLPAGAVPSDLAAGLARAARGDGVERIEEDGTVVFTAQAQDRLWHVCRELALP
                     LAPSDALARARVLRDLIAIRP"
     gene            complement(5004..5150)
                     /locus_tag="VIN75_12385"
     CDS             complement(5004..5150)
                     /locus_tag="VIN75_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975013.1"
                     /translation="MNQEKSQQPQSLELLEAEIEQVSGGAIGEIQRQYQQSLSDDNTK
                     AAAR"
     gene            5619..6881
                     /locus_tag="VIN75_12390"
     CDS             5619..6881
                     /locus_tag="VIN75_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8975014.1"
                     /translation="MTPQTPPPLFRVQALKHSSGASHGKVLLARPLSFTVLTALFVGI
                     AIAVVLFFACFSFTRKAQVAGVILPAHGVVRIASAQAGVVSSRAVHEGDHVVAGQVLF
                     GMSNERIGPAGVDVDRQISDLLSQQRRSLEVDQDKQGEQAVQRVEAARRRGDELAADL
                     RHIQAQQALQARRVALAQEAVARTQNLRRDGFVSAAALQDREADLLDQQQRAADLERA
                     ANSTQRDIEAARAQLRDAALQGQRDAQNGARSIASLDQQLVENKARREWFVRAEQDGV
                     ITGVTVEPGQRIAEDQPMATLLPAGEPLVAELYVPSRSAGFIKPGMVVQLRYQAFAYQ
                     KFGQHVGHVQEVSRTAMRPQDLPFSAATDSEPVYRVRVALDRPSVQAYGQDEPLRPGM
                     TVDASVLLDRRKLYEWVLEPLYSVMGKV"
     gene            6884..9037
                     /locus_tag="VIN75_12395"
     CDS             6884..9037
                     /locus_tag="VIN75_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEY8975015.1"
                     /translation="MTHAPDYAFWRRKRTPLILQTEAAECGLACLAMIAVHHGSHVDL
                     RSLRRRFPVSLKGVALKSLIAMAGSLGLQARPLKLDLDALSGLRLPCVLHWGMNHFVV
                     LTSVSRRRAVILDPAHGERRMPLSGVAEYFTGVALELTPGSDFRQEQPAPSFSLLSLM
                     GPIIGLRRGLAQLLLLGIALQVCALVAPFYMQWVVDEALVSADRELVTVLALGFLMLV
                     VVQSSITAVRSWVTTVLSTSLNFQWFGNAFAHLMRLPLPYFEKRHLGDIVSRFGSIQA
                     IQRSLTAQFVEGVIDGLLVFATLVVMFLYSVPLTLVACGAVGLYTLLRWTVFRALREA
                     TAQQIVHFARQQTFFLESARGIQSVRLFGRAQERRIGWMNALAEQFNADLRISKLSIS
                     YQTGNALLFNAERVLVIWLAALAVLDHRFSLGALFAFLGYKEQFSQRVASLVDKLFDL
                     RMLRLHGERVADILLTEPEEDDAFETDAAGIEPSIQVRNLSFRYSEGEPYVIDGLDLE
                     IPAGQCIAITGKSGCGKTTLVKLLMGLMEPTSGEILIGGVKLKQLGLSNYRHLVGTVM
                     QDDHLFSGTIEDNICFFDPAVDRGQVESCATQAAVHAEIVAMPMGYNTLIGDIGTGLS
                     GGQKQRILLARALYRRPRLLVLDEATSHLDNENEHAVNAAIRQIALTRIIVAHRSETI
                     AMAQRVVVLSGGRVERDLGPSEHRPVLAAGGGTPA"
     gene            complement(9050..9985)
                     /locus_tag="VIN75_12400"
     CDS             complement(9050..9985)
                     /locus_tag="VIN75_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975016.1"
                     /translation="MNASVRDVITPTYEVSQGLVYLRKLEVQVAFSCNLSCSGCVHGS
                     PASRAKFIDVGDLERDLAVLARHLRVLRFYLLGGEPLLHPQLHALLKVARASGVARQV
                     GILTNGVGLERMPDGFFRDIDFINVSRYPGVRLRFTAEEMDRKAERFGFRFALRDHDE
                     FMTSHLNARIESSGVVERIYTRCKSRSDWSTHTIADGRYYKCSRSPVLEERLRASGQC
                     VASRAGDSVPFRDNPDFVADLVRYLDSPTPMEACHWCLGSDGASFPHLLQNKRQAIAE
                     AGAIVHFSPARHLSAVARSELAGVLDADVAGPCDA"
     gene            complement(10059..>10448)
                     /locus_tag="VIN75_12405"
     CDS             complement(10059..>10448)
                     /locus_tag="VIN75_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY8975017.1"
                     /translation="VLPALLAALRDAHWPVREEAATTLAKLAPPAALDALLEAMRDDA
                     WQVRLRAARALGRLRQAPAVPTLVEALGHPLGNLRKEAALALGEIGAPDARPALEVAA
                     ADPDPEVRKSARLALSRLASDAPAAAA"
BASE COUNT         1514 a         3644 c         3835 g         1455 t
ORIGIN      
        1 cgcgcggcac tggcgatctg ctcgcgcagc agcgcgggcg agccggagaa gttgaccacg
       61 gtgagatcgc cgtgcgcgag cgcggcggtc tccagaaggc tgggcaatgc gcgggcgagt
      121 tcgacccggt cccgccaggt cacgacgatc gacagcatgg ggagtttcct ttcgatggtt
      181 tcgcgcggat gccggcgtca ggcgcgcaac gcgcgcgcga cgcgagccgc gatcgcgggc
      241 gcgtcgagcc cgtgctcggc gcggacctcg tcgtcgctgc cggacttgcc gcagaagtcg
      301 gcgggccagg tgatgccggt cacctcgccg aggccggccc agctgcgcgc atgcgaggcc
      361 aggccaccgt gcggcacgtg atcctcgatc acgaagctgc gctcgacgcc ggcgagcgcc
      421 agcccggcag gcaggtcggg cgtgatgcgc gccaggtgca gcacgcccac gtcgatgccc
      481 agcgcctcga gctcgcgcgc cgccgccatc gcccagtgcg tggccaggcc cgtggacacg
      541 agcagcgtcc tgcgcgccgg acgcaggaag cggaacgccg ccgcctcgcc gggcaaggca
      601 tcgcgctcgc ccagcggcgt gcgcggcagg cggatgtagg acggtgcctg cgagcgcccg
      661 acctcggcca gggcgaactc gacgtcggcc tggtcggccg gcgcccacac gtccatgtga
      721 ggcaggccca gcatgatcgc cacgtcgttc ggcgcggcgt gcgtcttgcc gttgcggccc
      781 gacaggccgc ccgcgtgcga cgcgacgagc gtcaccggca tcgacgcctg cgacaggctc
      841 acgcgcacct ggtcgtaggc gcgatagcag aggaacgcgg cgaacgagaa gacgctcgga
      901 cgccagccgc attcggccat gcccgcggcc acgcagacca tgttctgctc cgcgatgccg
      961 gcggcgaaga agcgctccgg gtgcgcgtgc gcgaagtgca tggcgccgtc cgagtcggcg
     1021 aggtcgccgt cgaggacgac gatgcgcggg tcgaggacgg cgcggcgcgc gagggcgtgc
     1081 ccggcgtggt cagcgagttt catggttcgg caggagatgg cgcagagtgg cgaggtgctc
     1141 ctcgtcggtc atcgcggcgc agtggaattc gtcggcgcgc gtctcggcga cgggtacgcc
     1201 ccggcccttg cgcgtgtggc agatgaccgc ggtcggggcg tgcgggtcgg cgtgccgggc
     1261 gcccagggcg gcgtcgatgg cggcgaagtc atggccgtcg cactcgacga cgttccaccc
     1321 gaaggcgcgc cacttctgcg ccgcccgcgt gagcggcgcg agcgccgtgc cgtcgcgatc
     1381 ccgcagcccc cactcctgca ggccgttgca gtcgaccacc gccgtgaggt tggcgatcgc
     1441 gttggccgag gcgaacatcg cggcttccca gacctggccc tcctggcatt cgccgtcgcc
     1501 caggacgacg aacacccggc ggcccgtctc gcgcagggcg atcgccatgc cgcagccgac
     1561 cgacaggccc tggccgaggg aaccggtgga gaagtcgacg ccgggcaccg tcttcatgtc
     1621 gggatggccc tcgaggatcg atgcgtgatc ggcgtaggtg cacaggtcgt cgtcgaagaa
     1681 gccgagccgg cgcagcaccg cgtacagcgc gacggcggcg tggcccttgg acagcagcag
     1741 ccggtcgcgg tcgggatggc gcggagcgcg cgggttcacg cggacgtggc cgcgatacag
     1801 cgtgagcagc acgtcgatga cgctgaggct gccgccgtag tggccgccgc cggcggcgtg
     1861 gagcgcgcgc acgatgtcgg ccctgagcag ggtggcctcg gcggcgcggc cgaggccggc
     1921 gggggccgtc tggacggcga cgtcgtcgct cgcctccagg tgcagcgggt ggggtgcgtt
     1981 catgcgggtg tccttccgat ccattcctcg acccaggccg ccagggtgat gacggcccag
     2041 gcctggtcca gcacgacccg cgattcgggc ggcgactggg gcgtcaaggc cgtcacgcca
     2101 tcgcggatgt ggcgcaggcc ctcttcgctc agcagcccga tctcggtcag gatgcagcgg
     2161 tccaggtagg ccatggcgat gtcggcgaac gccggatcct tcagccacag caggatcggc
     2221 gcgttgtacg acacgggctt gcgcgccatg atttcgggcg gcagcctgcg tgacgcgatc
     2281 tcgcgcaaca ggcgcttgtt gcgccaggtc tcgccttcgc gcgagaagcg ctcgctggcc
     2341 cccaggccgc atgcctggcg cgcgacggcg gtgtcgagga acgggtagac cgtggcgagc
     2401 gagcgcgagc gcgcgatggg ctcgaagtcg gcgaacagca agtcgggcac gcggtgctgg
     2461 cactcgtgcg cgaccatcaa ttcgaacagg tgcgccgacg gcttgatgcg gcggcagtcg
     2521 cgatagaacg ccgtcagcag cgcagcgaag tcccagccgg cgcagggctg ctgcaaccag
     2581 ccggcgagct gctcgtcgct gaagaagcgc ggcacgccct ggaacaggcg ccgtccggcc
     2641 tgcgccggct gccacagcat cgcgtcgaac ggatcgatgc gatcggcgaa ctcgaaggcg
     2701 gtctcgtgct cgcgctggag ctggtagtgg tcgaggaagc gcgagtagcc gccgaagacc
     2761 tcgtcggcgc ccaggcccga gtgcagcagg ccgtggccgt cgccgacgat gcgatcgagc
     2821 aggtggtgat gggtgacgct ggcccaggcg ctgcaaggct cggcggcgcg cagcaccttc
     2881 ggcagatggc gcgcggcgtc ggccggcgac atcggcacga gcgcgtggcg caagccggcc
     2941 tgcctggcca cgatcgccgc gtactcgcct tcgttgccgg gccagggcgc cgcgaactcg
     3001 acggtgtagg tgtcgagctg gtcggcgccg gcgtgctcgg cgagcgtcag gccgatgtag
     3061 gacgagtcga tgccgcccga cagcagcagc gcctggcggc cgggaacgct gcgcgcgagg
     3121 atcgcgtcct cgagcgtgcg ggcgatctcg tcgcggccgg cgtcgcccgc gaccttgcgc
     3181 gagccgtgcg acagcggcgt gaagtggcgc tgctcgatca gcggcgcgcc gggcgtccag
     3241 cgcagcaggt ggcccgcgcg cacgcgcgtc acgcggcgcg cgagcgtgcg acccggcacg
     3301 gggctcccga aggccaggta gtggtaggcg gactcgacgt cgagcgcgcg gtggaagcgc
     3361 ggatcgcgca gcagcggctc gagctcgctg cagacgacga agaatgcgac atcctcgcgc
     3421 acgaacagcg tctgctggcc gaacgcgtcg cgcagcaggg acagctcctg gcgcgcgccg
     3481 tcccagaaaa ccgcagcgtg gccgccttcg agcgtcgcga gcgcgcgcgg gccggcgacg
     3541 cacagctgtc gcagcatcgc ctcgtccgtc gtgcgttcac cgagcggcgt ccaaccggac
     3601 tcgggccggc ggacgcgccc gaccgccgcg aacgcgccgc aggcgtcgga gaccggcgca
     3661 tggatggcca ggccggcgtg cggctccagc caccaggcgt cgcgcgactg ggggaacgtg
     3721 gccggcaggg cggccgggag ttgggcagcc aggaagtcgc gcgccgcgga tgtcttggcg
     3781 acgagcgcgt agaggcagga ggcgtggctc acggtcggat cgcgatcagg tctcgcagca
     3841 cgcgggcacg cgcgagcgca tcggaggggg cgagcggcag tgcgagctcg cggcagacat
     3901 gccagagccg atcctgcgcc tgcgccgtga agacgacggt gccgtcctcc tcgatgcgct
     3961 ccacgccgtc gccgcgcgcg gcccgggcca ggccagcggc caggtcggac ggcacggcgc
     4021 ccgcgggcag gtccaggtcc acgcggccgt gctcgatgcg cacgggccat ccgccgtgca
     4081 ggccgagcgg gccgggcgcg gcggtgagca gcggcgcgcc gcccggcagc atcgcgcgga
     4141 tcaaggtggc ggcatgcatg gccgtcaact cgttgagacg gcggtccagc gcgatcggcg
     4201 cgtgcccgaa cagcagcggc gcggcatcca cttcgcggtc gccggcgaag atgcgcggca
     4261 gcggcagtcc gcccagcagc gccgcgtcgc tgcgcgccac ggcgccgaca tggaagtgat
     4321 gggcgacgac gcgcaacggc gcctcgcgct ccgcatggcc gagcgcggcg cgcgccagcg
     4381 cctgcaccat gccggcgttg ccgatcccga cgtcgggcgc gacgcccgat cggtgcagga
     4441 cgggatgcgt gaggtccgga tacgaacagt tgacgatcgg gcccgcgtga cccgcgtccc
     4501 gcgcggcggc ggtcacggcc gcgacgcaag gcagctgcgc gcacagctgc agcgcgaagc
     4561 cggcggcgcg cagcgcgacg gccgccgcat cttcccggcc gcccagcgcc cagggcgaca
     4621 ccagggccgc gcattgcacg atcagcgcgg gccgttcgcg cgcgacgagc tcggccacct
     4681 gcgcgcggtc gagcgcatcc agcggcacgc cgcgcgcggc gatgtcgccc aggctggacg
     4741 cgaagcgcgc cagcgcggcg gccacctcgg gtcgacggcc ggccaggacg agttcgacgc
     4801 cgccggcggt cgccagtcgg gtgacgatgc gctcggccag atcgccgagt ccgacgacga
     4861 gaatcttgtg gaacatggga tggcgctgcg gctcttgatg aatgccacgg cgggacagcg
     4921 acccgccgcg gcaaggtgcc cggcgaaccg ggacagcaca ccagggacgg atgcggacat
     4981 ccgccctttt caaggtcgcg tgatcagcga gcagcggcct tggtgttgtc gtccgacagc
     5041 gactgttggt attggcgctg gatctcgccg atcgcaccgc cgctgacttg ctcgatttcg
     5101 gcttcgagca gttcgagcga ttgcggttgc tgcgactttt cctggttcat ggaaactctc
     5161 cttggattga gaatgaggcc gcccggactc gggtcggcca tgcgcccgat cccccttggc
     5221 cgattcggcc gaggatcgtc gggcgcggcg gattgtgccc gcggctttcg agtgctgcac
     5281 ctgcggatcg atacccttgc aaattgatac ggatacaggc aagggtatgc gagaacaccg
     5341 tctgcgagag gcccgggacg aacaggtttc cgaagccgag atcttcgacc tgaacacccc
     5401 ctgaataggg gttccaaagg acgccgggac catgcgtgga tgccggagaa gcgcgcacgc
     5461 ggtgcgggcc tcggcccggc cgtgcgcggc atgatggttt gcaacaacgt cgcggtcgcg
     5521 catcccgttt cgacggcggc ccacgtgtac atttgcggcg attcgacatc cgccggcacc
     5581 cccgcgcacc tccgggaccg gcgcgaggcg ccacgagcat gaccccgcag acccctccac
     5641 ccctctttcg cgtccaggcc ctgaagcaca gcagcggcgc gtcgcatggc aaggtgctgc
     5701 tggcacgtcc gctgtcgttc accgtgctca cggccttgtt cgtggggatc gcgatcgccg
     5761 tcgtcctgtt cttcgcgtgt ttcagcttca cgcgcaaggc ccaggtggcc ggcgtcatcc
     5821 ttccggcgca cggcgtggtt cgcatcgcgt cggcgcaggc cggcgtggtg agcagccggg
     5881 ccgtccacga aggtgaccac gtggtcgccg ggcaggtgct gttcgggatg agcaacgagc
     5941 gcatcggccc ggccggcgtc gacgtcgacc ggcagatctc ggacctgttg tcgcagcagc
     6001 gcaggagcct cgaggtcgac caggacaagc agggcgagca ggccgtccag cgcgtcgagg
     6061 ccgcacgccg ccgcggcgac gagctcgcgg ccgacctgcg gcacatccag gcgcagcagg
     6121 cgctccaggc gcgccgggtc gcgctggcgc aggaggcggt cgcgcgcacg cagaacctgc
     6181 gacgggacgg attcgtctcg gcggccgcgc tgcaggatcg cgaggccgac ctgctcgacc
     6241 agcaacagcg cgccgccgac ctcgaacgcg cggccaactc gacgcagcgc gacatcgagg
     6301 ccgcgcgcgc gcagctgcgc gacgccgcgc tccagggcca gcgcgacgcg cagaacggcg
     6361 cgcgcagcat cgcctcgctc gaccagcagc tcgtggagaa caaggcacgg cgcgaatggt
     6421 tcgtgcgcgc ggagcaggac ggcgtcatca ccggcgtgac cgtcgagccc ggccagcgca
     6481 tcgccgagga ccagccgatg gcgacgctgc tgccggcggg cgagcccctg gtcgccgaac
     6541 tctacgtgcc gtcccgttcc gccggattca tcaagccggg catggtggtc cagctgcgct
     6601 accaggcctt cgcgtaccag aagttcggcc agcacgtcgg gcacgtgcag gaggtgtcgc
     6661 gcaccgcgat gcgtccccag gacctgccct tttccgccgc gaccgactcc gagcccgtct
     6721 atcgcgtgcg cgttgcgctc gaccgcccgt ccgtgcaggc ctatgggcag gacgagccgc
     6781 tgcgccccgg catgaccgtc gatgccagcg tgctgctcga tcgccgcaag ctctacgagt
     6841 gggtgctgga gccgctctac agcgtgatgg gcaaggtcta gcgatgacgc acgcccccga
     6901 ctacgcgttc tggcgccgca agcgcacgcc gctgatcctg cagacggagg ccgccgaatg
     6961 cggtctcgcc tgcctggcga tgatcgccgt ccatcacggc tcgcacgtcg acctgcgcag
     7021 cctgcgccgg cgcttcccgg tgtcgctcaa gggcgtggcc ctcaagagcc tgatcgcgat
     7081 ggcgggctcg ctcggcctgc aggcgcggcc gctcaagctc gacctcgacg cgctgtccgg
     7141 cctgcggctg ccctgcgtgc tgcactgggg catgaaccac ttcgtcgtgc tgacgtcggt
     7201 gagccggcgc cgcgcggtca tcctcgaccc ggcccatggc gagcggcgga tgcccctgtc
     7261 cggcgtcgcc gagtatttca ccggcgtggc gctcgaactc acgccgggca gcgacttccg
     7321 ccaggagcag cccgcgccgt cgttcagcct gctctcgctg atggggccca tcatcggact
     7381 gcgccgcggg ctggcgcaac tgctgctgct gggcatcgcg ctgcaggtgt gcgcgctcgt
     7441 cgcgccgttc tacatgcagt gggtggtcga cgaggcgctc gtctcggccg accgcgaact
     7501 cgtgaccgtg ctggcgttgg gctttctgat gctggtggtc gtgcaatcga gcatcacggc
     7561 cgtgcgctcg tgggtgacga ccgtgctgtc gaccagcctg aacttccagt ggttcggcaa
     7621 cgcgttcgcg cacctgatgc gcctgccgct gccctacttc gagaagcgcc acctcggcga
     7681 catcgtgtcg cgcttcggct cgatccaggc catccagcgc agcctgaccg cgcagttcgt
     7741 cgagggcgtg atcgacgggc tgctggtgtt cgcgacgctg gtcgtgatgt tcctctacag
     7801 cgtgccgctg acgctggtgg cctgcggcgc ggtcgggctc tacacgcttc tgcgctggac
     7861 ggtgttccgc gcgctgcgcg aggcgacggc gcagcagatc gtgcacttcg cgcgccagca
     7921 gaccttcttc ctcgagtcgg cgcgcgggat ccagagcgtg cggctgttcg ggcgcgcgca
     7981 ggagcggcgc atcggctgga tgaacgcgct ggccgaacaa ttcaacgccg acctgcgcat
     8041 ctccaagctg tcgatctcgt accagaccgg caacgcgctg ctgttcaacg ccgaacgcgt
     8101 gctggtcatc tggctggccg cgctggccgt gctcgaccac cggttctcgc tcggcgccct
     8161 gttcgcgttc ctcggctaca aggaacagtt cagccagcgc gtcgcctcgc tcgtggacaa
     8221 gctgttcgac ctgcgcatgt tgcgcctgca cggcgagcgc gtggccgaca tcctgctcac
     8281 cgagcccgag gaggacgatg cgttcgagac ggacgcggcg ggcatcgagc cgtccatcca
     8341 ggtgcgcaac ctctcgttcc gctattccga gggcgagccc tacgtgatcg acggcctgga
     8401 cctggagatc cccgccggcc agtgcatcgc gatcaccggc aagtccggct gcggcaagac
     8461 gacgctggtc aagctgctga tggggctgat ggaaccgacc tcgggcgaga tcctgatcgg
     8521 cggcgtgaag ttgaagcagc tgggcctgtc caactaccgg cacctggtcg gcacggtgat
     8581 gcaggacgac cacctgttct ccggcacgat cgaggacaac atctgcttct tcgatccggc
     8641 ggtcgaccgc gggcaggtcg agtcctgcgc cacccaggcg gcagtgcacg ccgagatcgt
     8701 ggcgatgccc atgggctaca acacgctgat cggcgacatc ggcaccggcc tgtcaggcgg
     8761 ccagaagcag cgcatcctgc tggcgcgagc cctctatcgc cgtccccggc tactggtgct
     8821 cgacgaagcg accagccacc tcgacaacga gaacgagcat gccgtcaacg ccgccatccg
     8881 gcagatcgcg ctgacgcgca tcatcgtcgc gcaccgcagc gagacgatcg cgatggcgca
     8941 gcgcgtggtg gtgctctcgg gcggccgggt cgagcgcgat ctcgggccct ccgaacatcg
     9001 acccgtgctc gcggccggcg gcggcacccc ggcctgacag gcgcgcgggt caggcgtcgc
     9061 agggtccggc gacgtcggcg tccagcacgc ccgccagttc gctgcgcgcc acggcggaca
     9121 agtgcctggc ggggctgaag tgcacgatgg cgcccgcctc ggcgatcgcc tgccgcttgt
     9181 tctgcagcag gtgggggaac gatgccccat ccgagccgag gcaccagtgg caggcctcca
     9241 tcggcgtggg ggaatcgagg taacgcacga ggtcggccac gaagtcgggg ttgtcgcgga
     9301 acgggacgct gtcgcccgcg cggctcgcga cgcactgccc tgacgcccgc agccgttcct
     9361 ccagcaccgg actgcgcgaa cacttgtagt agcggccgtc cgcgatcgtg tgcgtgctcc
     9421 agtcgctgcg ggacttgcag cgcgtgtaga tccgctcgac cacgccgctc gattcgatgc
     9481 gcgcgttcag gtgcgaggtc atgaactcgt cgtggtcgcg cagggcgaac cggaatccga
     9541 agcgttccgc cttccggtcc atctcctcgg cggtgaagcg caggcgcacg cccgggtagc
     9601 ggctcacgtt gatgaagtcg atgtcacgga agaacccgtc gggcatccgc tccagcccga
     9661 cgccgttcgt gaggatgccg acctgccggg ccacgccgct ggcccgcgcc accttgagca
     9721 gcgcgtgcag ctgcgggtgc aacagcggct cgccgcccag caggtagaag cgcaggacgc
     9781 gcaggtggcg ggcgaggacc gccaggtcgc gctccaggtc gccgacgtcg atgaacttcg
     9841 cgcggcttgc gggcgacccg tgcacgcacc cgctgcagct caggttgcag ctgaaggcca
     9901 cctgcacctc gagcttgcgc aggtagacga gtccctggct cacctcgtag gtcggggtga
     9961 tgacgtcgcg aacgctcgcg ttcacaaggc aacctccatc ctgcggactg cggtgtgacc
    10021 gacgcaggag ggaacgcctt cgggcggccg ggcggcgctc atgccgcggc ggccggcgcg
    10081 tcggacgcga gccggctcag cgcgaggcgc gcgctcttgc gcacctccgg atcgggatcg
    10141 gccgccgcca cttcgagcgc aggtcgcgcg tcgggcgcgc cgatctcgcc gagcgcgagc
    10201 gcggcctcct tgcgcaagtt gcccagcggg tggcccagtg cctcgaccag cgtaggaacg
    10261 gccggggcct ggcgcagccg gccgagcgcg cgggcggcgc gcaggcgcac ctgccaggcg
    10321 tcgtcgcgca tggcctccag caacgcgtcc agcgcggcgg gcggtgccag cttggccagc
    10381 gtcgtggcgg cttcctcgcg caccggccag tgcgcgtcgc gcagcgcggc caggagtgcc
    10441 ggcaggac
//
LOCUS       DATCRO010000456         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_646272, whole genome shotgun sequence.
ACCESSION   DATCRO010000456 DATCRO010000000
VERSION     DATCRO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646272"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VIN75_12410"
     CDS             complement(<1..1077)
                     /locus_tag="VIN75_12410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8975018.1"
                     /translation="MKVIKLRAGKERSLSRHHPWVFQGSIEKGKADVGETVRVEASDG
                     SFLAWGAYSPESLIRVRAWSWEQSERIDAAFFERKLATALALRERLAVASDGVRLVHG
                     EADGLPGLVVDRYDDILSAQFLAAGVERWKQTIADLLVKLTGCTKIYERSDASVRSLE
                     GLPSVRAWLRGGSDDPAASVVTIHESGWKLTVDVAEGHKTGYYLDQRENRARFAQLVR
                     QYGCKSVLNCFSYTGGFSVAALAGGATRVISVDSSAPALALASAHVKLNGFDAKRHEA
                     RDADVNALLRELLQGGERFDAIVLDPPKFAPTAAHADRASRAYKDINRLALKLLKPGG
                     LLLTFSCSGGISADLFHKIVAGAAM"
     gene            complement(1171..1824)
                     /locus_tag="VIN75_12415"
     CDS             complement(1171..1824)
                     /locus_tag="VIN75_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975019.1"
                     /translation="MSGVFAEPATEIPLPVSVRFDIRNHSDVMVQTFGEVCFISNDDL
                     AWPLIRSDSRDASRLHAALGAPALMVAFCHYDANGNHGYAIFEHGRRVRTRLQSSGEP
                     GAHELLQSGTPQLFEQRWLGARHYMEAEAAAGADDARMFFLGEREVVVPGRDLTGRML
                     QDGLDAMFGVCPWETLLTPTYRFFRLGTERAREAVDVPAVSRARAIPASKRPWWQFL"
     gene            complement(1974..>3055)
                     /locus_tag="VIN75_12420"
     CDS             complement(1974..>3055)
                     /locus_tag="VIN75_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8975020.1"
                     /translation="YWLQLWNFLLGLGVSWIILRGGLSARVRDACARLGRAKLARDAL
                     YGAFFAAASWVLTLPLAIYQGWARERQYGMATQTFGPWFVDQLKELGVTVVAFALGAA
                     LFYAVMRRARERWWIWGTVVTMAMLVFIIMVAPVVIDPLFNTYKPVTDPAVKSAVLQM
                     ARADGVPVDNVYEFDASQQTTRVSANVSGILGTAAVRLNDNLLRTSLPEIRGVMGHEL
                     GHYVMNHMYKGLCEMAILILAGFLFAKWAMDALLRRSAAAWGLEGVADVAALPLLLAV
                     FSVWMFVTTPISNSLIRTQEIEADRFGLNLAREPNGFAEAALKLTEYRKPDPGPIEEI
                     VFFDHPSTRHRIHDAMQWRQAMGTP"
BASE COUNT          459 a         1075 c         1092 g          429 t
ORIGIN      
        1 catcgcggcg ccggccacga tcttgtggaa caggtcggcg ctgatgccgc ccgagcacga
       61 gaacgtcagc agcaggccgc ccggcttgag cagcttcagc gcgaggcggt tgatgtcctt
      121 gtaggcgcgc gacgcgcgat cggcgtgggc cgcggtcggc gcgaacttcg gcgggtcgag
      181 cacaatcgcg tcgaagcgct cgccgccctg cagcagctcg cgcagcagcg cgttgacgtc
      241 ggcgtcgcgg gcctcgtgcc gcttcgcgtc gaacccgttg agcttgacgt gcgcgctcgc
      301 cagcgcgagc gcgggcgcgg acgaatcgac gctgatcacg cgcgtcgcgc cacccgccag
      361 cgcggccacc gagaagccgc ccgtgtagct gaagcagttc agcaccgact tgcagccgta
      421 ctgcctgacg agctgcgcga agcgcgcgcg gttctcgcgc tggtccaggt agtagccggt
      481 cttgtggccc tcggcgacgt cgacggtcag cttccagccg ctctcgtgga tcgtgacgac
      541 cgaggccgcc ggatcatcgc tgccgccgcg cagccaggcg cgcacggacg gcagcccctc
      601 gagcgaacgc acgctggcgt cgctgcgctc gtagatcttc gtgcagccgg tgagcttgac
      661 cagcaggtcg gcgatcgtct gcttccagcg ctcgacgccg gctgccagga actgcgcgga
      721 caggatgtcg tcgtagcggt cgaccaccag gcccggcagc ccgtcggcct cgccgtgcac
      781 cagccgcacg ccgtcgctcg cgacggcgag gcgctcgcgc agcgccagcg cggtggccag
      841 cttgcgctcg aagaacgcgg cgtcgatgcg ctcggactgt tcccagctcc aggcccgcac
      901 gcggatcagc gactcggggc tgtacgcgcc ccacgcgagg aagctgccgt cgctggcctc
      961 gacccgcacc gtctcgccga cgtcggcctt gcccttctcg atgctgccct ggaacaccca
     1021 cgggtgatgg cgggagagcg agcgttcctt gccggcgcgg agcttgatga ctttcatggg
     1081 ctcgattgtc cccgacgacg gggcgggccg cgtgccgccc cgacgccctc ctgctgcccg
     1141 cgtgcgctca gccgggaggc cgctgcgcct tcagaggaac tgccaccacg gccgcttgct
     1201 cgcggggatg gcccgggcgc gcgagacggc cggcacgtcg acggcttcgc gcgcgcgctc
     1261 cgtgcccagg cggaagaagc ggtaggtggg cgtgagcagc gtctcccacg ggcagacgcc
     1321 gaacatcgcg tccaggccgt cctgcagcat gcggcccgtc aggtcgcggc cgggcaccac
     1381 cacctcgcgc tcgcccagga agaacatgcg ggcgtcgtcg gccccggcag cggcctcggc
     1441 ctccatgtag tggcgcgcgc cgagccagcg ctgctcgaac agctgcggcg tgccgctctg
     1501 cagcagctcg tgcgcgccgg gctcgccgct gctctgcagg cgggtgcgca cgcgccggcc
     1561 gtgctcgaag atcgcgtagc cgtggttgcc gttggcgtcg tagtggcaga acgccaccat
     1621 cagcgcgggg gcgccgagcg cggcgtgcag gcggctcgcg tcgcgcgaat cgctgcggat
     1681 cagcggccag gccaggtcgt cgttgctgat gaagcagacc tcgccgaagg tctgcaccat
     1741 cacgtccgaa tggttgcgga tgtcgaatcg caccgacacg ggcagcggga tctcggtggc
     1801 cggttccgcg aagacgcccg acatgaacgc ctccagatcc gtcaccgaat cggaaatgcg
     1861 aaacgcgatg ccggcctgtg cgatgcccat gcgcgaaggt taccggcgga gcgccgcccg
     1921 cggaggccgg aatctgcggc gcagttcacg cccggcggcc cggcgagggg ccttcagggc
     1981 gtgcccatcg cctgccgcca ctgcatggcg tcgtggatgc ggtggcgcgt cgacgggtgg
     2041 tcgaagaaga cgatctcctc gatcgggccc ggatcgggct tgcggtattc ggtcagcttg
     2101 agcgcggcct cggcaaagcc gttcggctcg cgcgcgaggt tcaggccgaa gcggtcggcc
     2161 tcgatctcct gcgtgcgaat cagcgagttg ctgatcggcg tggtgacgaa catccagacg
     2221 gagaacaccg ccagcagcag cggaagcgcg gcgacgtcgg ccacgccctc cagcccccag
     2281 gccgcggcgc tgcggcgcag cagcgcgtcc atggcccact tggcgaacag gaagcccgcc
     2341 aggatgagga tcgccatctc gcacaggccc ttgtacatgt ggttcatcac gtagtggccc
     2401 agctcgtggc ccatgacgcc gcggatctcg ggcagcgacg tgcgcagcag gttgtcgttg
     2461 agccgcacgg cggcggtgcc gaggatgccc gacacgttgg cgctgacgcg cgtcgtctgc
     2521 tgcgaggcgt cgaactcgta gacgttgtcg accggcacgc cgtcggcgcg cgccatctgc
     2581 agcacggcgg acttgaccgc gggatcggtc acgggcttgt aggtgttgaa cagcggatcg
     2641 atgacgaccg gtgcgaccat gatgatgaag acgagcatgg ccatcgtcac cacggtgccc
     2701 cagatccacc agcgctcgcg ggcgcggcgc atgaccgcgt agaacagggc ggcgccgagc
     2761 gcgaacgcca cgaccgtcac gcccagctcc ttgagctggt cgacgaacca tgggccgaag
     2821 gtctgcgtgg ccatcccgta ctggcgctcg cgcgcccagc cctggtagat ggccagcggc
     2881 agcgtcagca cccagctggc ggcggcgaag aaggcgccgt agagcgcgtc gcgcgccaac
     2941 ttggcgcgcc cgaggcgcgc gcaggcgtca cgcacgcgcg cggacagccc gccgcgcagg
     3001 atgatccagc tcacgcccag cccgagcagg aagttccaca gctgcagcca gtagc
//
LOCUS       DATCRO010000457         5015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_432482, whole genome shotgun sequence.
ACCESSION   DATCRO010000457 DATCRO010000000
VERSION     DATCRO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432482"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VIN75_12425"
     CDS             complement(<1..628)
                     /locus_tag="VIN75_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975021.1"
                     /translation="MLRRLWPLATLYCGRLGTALVGILLLPWFSRLMGPQQFGVVAII
                     LSTQSLLVALDLGLSIVLGRDLAGLAAGDLPLGRAMFAHAERTLLRLYAGLLVLALVA
                     SRFMAVPLAAPDLVAVAVLFGAVVHQNLGQVALLARQDYAWVGLNQLIGVLLRNAFTL
                     ACLSMLAPSLTVFVASQALGACVHALVTRWRLARRLPPAPAGAHPAFRA"
     gene            complement(640..1758)
                     /locus_tag="VIN75_12430"
     CDS             complement(640..1758)
                     /locus_tag="VIN75_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8975022.1"
                     /translation="MSAPIVLFDCRPEPAMLEALAPVLASGMLASGPNVAALEAAIAQ
                     RVQRPDVVCLGDMTHALALALQLAGVGPGDDVLTLSYDCMSSNSAIAHVGATPVWVDI
                     DGATATMSPADCERAITPRTKALVVYHVAGYPAPLHELRAFCDRRGIALVEDANNALG
                     ASLDGRPIGSFGDFAVFSFYANRQVNGIDGAALVCRDAACAAEARRLRRFGIDLTTFR
                     DPRGEIDPASDIPQVGMSSPLNHVQATLALAHLGTLDARLERNRRHVDVLASRLADVA
                     GLRPVTWAPGAQPVFWTWLVRCDGRDGVLERLKARGVQCSKLHQPNHVYSGFASAPRA
                     LPGTDRFIDEVLGLPCGWWLDDAALDRLCGEIRTACGA"
     gene            complement(1755..2840)
                     /locus_tag="VIN75_12435"
     CDS             complement(1755..2840)
                     /locus_tag="VIN75_12435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8975023.1"
                     /translation="MLPLVKVSMPPRETLMPALESVLYGGMIAEGEHVYRFEAEFAAT
                     FGLPNALATSSGTGALHIALLLAGVQPGDEVITTSMTAEPTNTTILQVGATPVFADVD
                     AGNGNLSPEAVQAAVTGRTRAILVVHYAGYPVDLARMREIADRHGLRLIEDCAHALGA
                     RFGGEPIGTIGDSAIFSLQAIKHMTTVDGGVLTVKDASTLPLARRLRWFGLTKGVPRT
                     EVDITTPGFKYNMNNVTATIGLLQLQGIRAVIGAHVANGQYYDQALAGIAGLAPARIV
                     PAAEPSYWLYTLLADDSDDVERRLAAIGVSASKLHRPNHFHSVFAPHRRPLPQLDEFY
                     RRLVHVPCGWWVTADDRARIVDALRKG"
     gene            complement(2918..3250)
                     /locus_tag="VIN75_12440"
     CDS             complement(2918..3250)
                     /locus_tag="VIN75_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HEY8975024.1"
                     /translation="MPSRQDQLQEDTYFRVMRLLQEDPGMTQREMARRLGVSLGGINY
                     CLQALVDKGWIKIQNFQRSERKMAYAYLLTPSGLASKAALTARFLQRKMQEFEALKAE
                     IQSLQNDS"
     gene            complement(3409..4551)
                     /locus_tag="VIN75_12445"
     CDS             complement(3409..4551)
                     /locus_tag="VIN75_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY8975025.1"
                     /translation="MQHDDHVSPGQASGFPGSAAFEDEDDGIDFGALLARARRHWREL
                     AGTTVAACAIGYGISFVVTPLYTSHTTLIPPQQQQQGGAAAALSSLGVLSSLAGGAMK
                     SPVEQYISLMESDAVTDRLIDRFGLMKVYDYKYRDSTRKKLLKRSEITAGKKDGLITV
                     KVEDSDPKRAAAMANQYVEELRHMTSTLAVTEAQQRRMFFQNQMQEARNKLAEAQSAL
                     QQSGFGQGALNAAPQTAAAGYARLRTELASAQVRLATLQGSLAPGAPEVQQQSTMVAA
                     LQDQLRQLEANPDAKANGPDYVTKYREFKYEETVFELMAKQYELARVDEAKEGAIIQV
                     VDPAQPPERRSFPRRLYFAAGGAAAGLLLGAALLFFRERRTQAAAA"
     gene            complement(4556..>5015)
                     /locus_tag="VIN75_12450"
     CDS             complement(4556..>5015)
                     /locus_tag="VIN75_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY8975026.1"
                     /translation="RQVKPTGRVVLQLAPDAATLPDLPLEDGDTLSIPQRGTSVGIFG
                     SVFNTGSFVYEPGHTTQQYLALAGGPTRSADKGSIFMIRANGSVISAQQGSSFWHTGN
                     NFNSATVQPGDTIFVPEELNKSTFVQDAKDWTQILYQFGLGLAGINALGL"
BASE COUNT          697 a         1732 c         1864 g          722 t
ORIGIN      
        1 ccgcccggaa cgccggatgc gcccccgccg gcgccggcgg caggcgccgc gcgagacgcc
       61 agcgcgtgac gagcgcgtgc acgcaggcac ccagcgcctg cgaggcgacg aacacggtca
      121 ggctcggcgc cagcatcgac aggcaggcca gcgtgaaggc attgcgcagc agcacgccga
      181 tcagctggtt caggccgacc cacgcgtagt cctgccgcgc cagcagcgcg acctgcccca
      241 ggttctggtg gacgaccgcg ccgaacagca cggcgacggc cacgaggtcg ggcgcggcca
      301 gcggcacggc catgaaccgg ctcgcgacca gcgccagcac cagcaggccg gcgtacaggc
      361 gcagcagcgt gcgttcggcg tgcgcgaaca tcgcgcggcc cagcggcagg tcgccggcgg
      421 ccaggcccgc gaggtcgcgc cccagcacga tggacagccc caggtcgagc gccaccagca
      481 gcgactgcgt ggacaggatg atcgcgacca cgccgaactg ctgcggcccc atcagccggc
      541 tgaaccacgg cagcagcagg atgccgacca gcgcggtgcc caggcggccg cagtacagcg
      601 tcgccagggg ccagagtcgg cgcagcatcg tcatcggtct caggcgccgc aggccgtccg
      661 gatctcgccg cacagccgat ccagcgccgc gtcgtccagc caccagccgc agggcaggcc
      721 cagcacctcg tcgatgaacc ggtccgttcc cggcagcgcg cgcggcgcgc ttgcgaagcc
      781 cgagtagacg tggttgggct ggtgcagctt cgagcactgc acgccgcgtg ccttcaggcg
      841 ttcgagcacg ccgtcgcggc cgtcgcagcg caccagccag gtccagaaca cgggctgcgc
      901 gccgggcgcc caggtgaccg ggcgcaggcc ggcgacgtcg gccaggcggg acgccagcac
      961 gtcgacatgg cgccggttgc gctcgaggcg cgcgtccagc gtgcccaggt gcgccagcgc
     1021 cagcgtggcc tgcacgtggt tcagcggcga ggacatgccg acctgcggga tgtcggaggc
     1081 cgggtcgatc tcgccgcgcg ggtcgcggaa ggtggtcagg tcgatgccga agcggcgcag
     1141 gcggcgcgcc tcggcggcgc aggccgcgtc gcggcacacg agcgccgcgc catcgatgcc
     1201 gttgacctgg cggttcgcgt agaacgagaa caccgcgaag tcgccgaagc tgccgatcgg
     1261 ccggccgtcc aggctcgcgc cgagcgcgtt gttggcgtcc tcgacgagcg cgatgccgcg
     1321 gcggtcgcag aacgcgcgca gctcgtgcag cggcgccggg tagccggcca cgtggtagac
     1381 gacgagcgcc ttggtgcgcg gcgtgatcgc gcgctcgcaa tcggccggcg acatcgtcgc
     1441 ggtcgcgccg tcgatgtcga cccagaccgg cgtggcgccg acgtgggcga tggccgaatt
     1501 ggaggacatg cagtcgtacg acagcgtcag cacgtcgtcg cccgggccga cgcccgcgag
     1561 ctgcagcgcc agcgcgagcg cgtgcgtcat gtcgccgagg cagaccacgt ccgggcgctg
     1621 cacgcgctgc gcgatggccg cctcgagcgc cgccacgttg gggccgctgg cgagcatgcc
     1681 gctcgcgagc accggcgcca gcgcctcgag catcgcgggc tcgggccggc agtcgaacag
     1741 gacgatcggg gcgctcatcc cttgcgcagc gcatcgacga tgcgcgcccg gtcatccgcg
     1801 gtcacccacc agccgcacgg gacgtgcacc aggcggcgat agaactcgtc gagctgcggc
     1861 agcggccggc gatgcggcgc gaagacgctg tggaaatggt tcggccggtg cagcttggac
     1921 gccgacacgc cgatggcggc gaggcgccgc tcgacgtcgt cggaatcgtc ggccagcagc
     1981 gtgtagagcc agtagctggg ctcggcggcg ggcacgatgc gcgcgggcgc caaccccgcg
     2041 atgccggcca gcgcctggtc gtagtactgt ccgttggcga cgtgcgcccc gatcaccgcg
     2101 cggatgccct gcagctgcag caggccgatc gtcgcggtga cgttgttcat gttgtacttg
     2161 aagcccggcg tcgtgatgtc gacctcggtg cgcggcacgc ccttggtcag gccgaaccag
     2221 cgcaggcggc gcgccagcgg cagcgtcgat gcgtccttga ccgtcagcac cccgccgtcc
     2281 acggtggtca tgtgcttgat ggcctgcagc gagaagatcg ccgagtcgcc gatggtgccg
     2341 atcggctcgc cgccgaagcg cgcgccgagc gcgtgggcgc agtcctcgat cagccgcagg
     2401 ccgtggcggt cggcgatctc gcgcatgcgc gccaggtcga ccggatagcc cgcgtagtgc
     2461 accaccagga tggcgcgcgt gcggccggtg acggcggcct gcacggcctc gggcgacagg
     2521 ttgccgttgc cggcgtcgac gtcggcgaac accggcgtgg cacccacctg caggatcgtc
     2581 gtgttggtcg gctcggccgt catcgaggtg gtgatgacct cgtcgcccgg ctgcacgccc
     2641 gccagcagca gcgcgatgtg cagggcgccg gtgccgctgc tggtggccag cgcgttgggc
     2701 aggccgaagg tcgccgcgaa ctccgcttcg aagcggtaga cgtgctcgcc ctcggcgatc
     2761 atgccgccgt acaggacgct ctcgagggcg ggcatcagcg tctcgcgcgg cggcatcgag
     2821 accttgacga ggggaagcat cggcgcggcg gtttgcatcg ggaggtggtg tcggatcggc
     2881 cggagggcgg agcgggcgcg cagcggggcg gcgtccgtca ggaatcgttc tgcagggact
     2941 ggatctccgc cttgagcgcc tcgaattcct gcatcttgcg ttgcaggaag cgcgcggtca
     3001 gggcggcctt gctggccagt ccgctcgggg tcaggaggta ggcgtaggcc atcttgcgct
     3061 cgctgcgctg gaagttctgg atcttgatcc agcccttgtc gacgagcgcc tgcaggcagt
     3121 agttgatgcc gccgaggctc acgccgagcc gccgcgccat ctcgcgctgc gtcatgcccg
     3181 gatcctcctg cagcaggcgc atcacgcgga agtacgtgtc ctcttggagc tggtcctggc
     3241 ggctgggcat cggcgactgt tgttcaagtt cgaacacagt gtagcagcaa cgctttcggc
     3301 gggcggtgcc ggttcctgcg ccggacaggg tccgttcagg cgtggcgaca gcaggccgcc
     3361 ggccaggagc gccggcgccc ggcgcccggc gcccggcgcc gcgccgcgtc aggcggcggc
     3421 ggcctgcgtc cggcgctccc ggaagaacag cagggccgcc cccagcagca gcccggcggc
     3481 cgcgccgccg gcggcgaaat agagccggcg cgggaaggaa cggcgctcgg gcggctgcgc
     3541 cgggtcgacg acctggatga tggcgccttc cttggcctcg tcgacgcgcg cgagctcgta
     3601 ctgcttggcc atcagctcga acaccgtctc ctcgtacttg aactcgcggt acttggtgac
     3661 gtagtcgggg ccgttggcct tggcgtcggg gttggcctcg agctggcgca actggtcctg
     3721 gagcgcggcc accatcgtgg actgctgctg cacttcgggc gcgcccggcg cgagcgagcc
     3781 ctgcagcgtg gccaggcgca cctgcgcgga cgcgagctcg gtgcgcaggc gcgcatagcc
     3841 ggccgccgcg gtctgcggcg cggcgttcag ggcgccctgc ccgaagccgc tctgctgcag
     3901 ggccgactgc gcctcggcga gcttgttcct ggcctcctgc atctggttct ggaagaacat
     3961 gcggcgctgc tgcgcctcgg tcaccgccag cgtcgaggtc atgtggcgca gctcctcgac
     4021 atactggttg gccatcgcgg ccgcgcgttt cgggtcgctg tcctccacct tgacggtgat
     4081 caggccgtcc ttcttgccgg cggtgatctc ggagcgcttg agcagcttct tgcgcgtcga
     4141 gtcgcggtac ttgtagtcgt agaccttcat caggccgaag cgatcgatca gccggtccgt
     4201 gacggcgtcg ctctccatca gcgagatgta ctgctcgacg gggctcttca tggcgccgcc
     4261 cgccaggctg ctcagcacgc ccagcgacga cagcgcggcc gctgccccgc cttgctgctg
     4321 ctgctgcggc gggatcagcg tcgtgtgcga ggtgtacagc ggcgtcacca cgaagctgat
     4381 gccgtagccg atcgcgcacg cggcgacggt cgtgcccgcc agctcgcgcc agtgtcggcg
     4441 cgcccgggcg agcagggcgc cgaagtcgat gccgtcgtcc tcgtcctcga aggcggcgct
     4501 gccggggaag ccggaagctt gtccgggcga gacgtgatcg tcgtgttgca tcgcgtcaga
     4561 ggcccagtgc gttgatgccg gccaggccga ggccgaactg gtagaggatc tgcgtccagt
     4621 ccttggcgtc ctgcacgaag gtcgacttgt tcagttcctc tggcacgaag atggtgtctc
     4681 ccggctgcac ggtggcgctg ttgaagttgt tgccggtgtg ccagaacgac gagccctgct
     4741 gcgcgctgat caccgagccg ttggcgcgga tcatgaagat gctgcccttg tcggcgctgc
     4801 gcgtcgggcc gccagccagc gccaggtact gctgggtggt gtgacccggc tcgtagacga
     4861 agctgccggt gttgaacacg ctgccgaaga tgcccaccga ggtgccgcgc tgcgggatcg
     4921 acagggtgtc gccgtcctcc agcggcaggt ccggcagggt cgccgcgtcg ggcgccagct
     4981 gcaggacgac acggccggtg ggcttgacct ggcgc
//
LOCUS       DATCRO010000458         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_623668, whole genome shotgun sequence.
ACCESSION   DATCRO010000458 DATCRO010000000
VERSION     DATCRO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623668"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2666
                     /locus_tag="VIN75_12455"
     CDS             <1..2666
                     /locus_tag="VIN75_12455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8975027.1"
                     /translation="SDAPEQFIELVEAGPELEVLDEGERLRVRLQPALRVTPDAATPR
                     WAGSEAEAKELEALRLVSIWRDGPQRAKVIRLSAAQKRVAQLLGPAGLDIPRQGAAQL
                     QEVLTGLGSHFRIHADDAGAAQAAREVEAETRLRAELQPVGDGLQLRLVATPFGADYA
                     AVGPRLIPGRGRARVVATLKGESVGVQRDLQREREHLESVLDTCPMLPPLPLDAPCEW
                     LLDAADDALALLERLQPHTSILALDWPQGRPVRVDPGALGQLALKVRTHEDWLALDGK
                     LKVDEELVVGLQQLITFASEGKSRFMPLGDGRFLALTQELRERVADIAAVVEKQTARA
                     NNPDEVRAPAIAAPWLREAMEGARVEHDAAYAARLQALDDARRVVPALPSTLQAQLRP
                     YQEEGFEWAMRLASAGLGACLADDMGLGKTLQALAVLLARAANGPALVVVPTSLIGNW
                     RSEALRFAPSLRVHVYGENVSWSPGPPSTSDSPRGTGGLAPERPRAAAARAVGGRASI
                     VGEMGRHDVLLVSYPMLQIDGEAFAGKVWATLVLDEAQAIKNAAAKRSQAVFALQATF
                     RLAMSGTPIENRLGELWSVMRACNPGLLGTLPRFNERFANLIERQGDRHAQRTLKRLI
                     APFILRRTKAQVLADLPPRTELVLQVQGDDAEKAHYEALRREALVAAEKSLSGDAAGQ
                     AHLNILAGLTRLRRAACDPRLVTPTLKRPGAKVTAFAELAAELVANGHKALVFSQFVD
                     FLGLLREPLDAAGIAYQYLDGSTPAAERTRRVEAFQEGEGDLFLISLKAGGFGLNLTV
                     ADYVVIADPWWNPAAEDQASGRAHRIGQQRPVTVYRLVNAGTLEERILTLHQTKRELA
                     DSVLESDGQAATTVPLARELLQLMRGDAMPE"
     gene            2726..3172
                     /locus_tag="VIN75_12460"
     CDS             2726..3172
                     /locus_tag="VIN75_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8975028.1"
                     /translation="MTDVTGIDHIYITVSELARSEPFYDRVLRDALGFRKNTFTLGGD
                     PHIQYYNRLFGFVLRPARVQRAHEPYSPGLHHFCLRVDSVEDVQTVARDLQAAGIAAT
                     DARFHPEYSDDYCATFFEDPDGVRLEVTNWRLERRQRAAHWDDPQP"
     gene            3215..3712
                     /locus_tag="VIN75_12465"
     CDS             3215..3712
                     /locus_tag="VIN75_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975029.1"
                     /translation="MPSSPPPDRPNRLSELPGSPLRAVLTGAAIDICGSRVINIVAVT
                     IYAMQLVTPGMTDEQAADLLQKTPPSFTLLLVLTVLGMLMSVAGGYACARVVLRDEWR
                     TGAMLAAVAVVFGLFIDDAGVSDLALLYVLCDVAGLLLGVKYGAERNRRLEAPAPSPA
                     DASAP"
     gene            3709..4269
                     /locus_tag="VIN75_12470"
     CDS             3709..4269
                     /locus_tag="VIN75_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HEY8975030.1"
                     /translation="MNGRRVAIGLGVVAALGAGAWWAVAMFAPQAFIDIDQAQLQQRL
                     DGYFPQKHCTMMVACVTLSQPVVKLTEGSPRIGLSSGILVTLGHREMPGQVTFSGVLR
                     YVRYQGDFYLDDVQIENFALTGVSPDMVQVVKQRGPDALRRALQGHPIYTLRGDSARQ
                     ALAKLAVRNVQVVDGKVRVTFLSFGG"
     gene            4402..4489
                     /locus_tag="VIN75_12475"
     tRNA            4402..4489
                     /locus_tag="VIN75_12475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4436..4438,aa:Ser,seq:cga)
BASE COUNT          650 a         1635 c         1583 g          621 t
ORIGIN      
        1 tcagcgatgc gcccgagcag ttcatcgagc tggtggaggc cggccccgag ctggaggtgc
       61 tggacgaagg cgaacgcctg cgcgtgcgcc tgcaacccgc gctgcgcgtg acgcccgacg
      121 ccgccacgcc gcgctgggcc ggcagcgagg ccgaggcgaa ggagctcgag gcgctgcgcc
      181 tcgtctcgat ctggcgcgac ggcccgcagc gcgcgaaggt catccgcctg agcgcggcgc
      241 aaaagcgcgt ggcgcagctg ctgggcccgg cgggcctgga catcccgcgc cagggcgccg
      301 cgcagctgca ggaggtgctc acgggcctgg gcagccattt ccgcatccac gccgacgacg
      361 ccggcgccgc gcaggccgcg cgcgaggtcg aggccgagac gcggctgcgc gcggagttgc
      421 agccggtggg cgacggcctg cagctgcgcc tggtcgccac gccgttcggc gccgactacg
      481 ccgccgtcgg cccgcgcctg atcccgggcc gcggccgggc gcgcgtggtg gccacgctca
      541 agggcgagag cgtgggcgtg cagcgcgatc tccagcgcga gcgcgagcat ctggagtctg
      601 tgctcgacac ctgcccgatg ctgccgccgc tgccgctgga cgcgccgtgc gaatggctgc
      661 tggacgccgc cgacgacgcg ctcgcgctgc tggagcgcct gcagccgcac acgtccatcc
      721 tggcgctcga ctggccgcag ggccggccgg tgcgcgtcga tccgggtgcg ctcggccagc
      781 tcgcgctcaa ggtgcgcacg cacgaggatt ggctggcact ggacggcaag ctcaaggtcg
      841 acgaggagct cgtcgtcggc ctgcagcagc tgatcacttt cgcgagcgaa ggcaagagcc
      901 gcttcatgcc gctcggcgac ggccgcttcc tcgcgctgac gcaggagctg cgcgaacgcg
      961 tggccgacat cgccgccgtc gtcgagaagc agacggcgcg cgcgaacaat cccgacgagg
     1021 tgcgcgcccc ggcgatcgcc gcgccgtggc tgcgcgaggc gatggagggc gcgcgcgtcg
     1081 agcacgacgc cgcgtatgcc gcgcgcctgc aggcgctcga cgacgcgcgc cgcgtcgtgc
     1141 cggcgctgcc gtcgacgctg caggcgcagc tgcggccgta ccaggaagaa ggcttcgagt
     1201 gggcgatgcg gctcgcgtcc gccggcctgg gcgcgtgcct ggccgacgac atgggcctgg
     1261 gcaagacgct gcaggcgctg gcggtgctgc tggcgcgcgc cgccaacggg cccgcgctgg
     1321 tggtggtgcc gacgtcgctg atcggcaact ggcgttccga ggcgctgcgc ttcgcgccgt
     1381 cgctgcgcgt gcatgtctac ggcgagaacg tgtcatggag ccccggtccg ccgagtacgt
     1441 cggacagccc ccgagggaca ggcggccttg ctccggagcg gccgcgcgcg gcggccgcca
     1501 gggccgtcgg aggccgcgca tccatcgtcg gcgagatggg caggcatgac gttctgctcg
     1561 tctcataccc gatgctgcag atcgacggcg aggcgttcgc cggcaaggtg tgggcgacgc
     1621 tggtgctgga cgaggcgcag gccatcaaga acgccgcggc caagcgcagc caggcggtgt
     1681 tcgcgctgca ggcgacgttc cgcctggcga tgtcgggcac gcccatcgag aaccggctgg
     1741 gcgagctctg gtcggtcatg cgggcctgca acccgggcct gctgggcacg ctgccgcgct
     1801 tcaacgaacg cttcgccaac ctcatcgaac gccagggcga ccgccacgct cagcgcacgc
     1861 tcaagcgcct gatcgcgcca ttcatcctgc gccgcaccaa ggcccaggtg ctggccgacc
     1921 tgccgccgcg caccgaactc gtgctgcagg tgcagggcga cgatgccgag aaggcgcact
     1981 acgaggcgct gcggcgcgag gcgctggtgg ccgcggagaa gagcctgtcc ggcgacgccg
     2041 cgggccaggc gcacctgaac atcctggccg gcctgacgcg cctgcgccgc gccgcgtgcg
     2101 acccgcgcct ggtcacgccc acgctcaagc gccccggcgc gaaggtgacc gcgttcgccg
     2161 agctcgccgc cgagctcgtc gccaacggcc acaaggcgct cgtcttcagc cagttcgtcg
     2221 acttcctggg cctgctgcgc gaaccgctgg acgccgcggg catcgcttac cagtacctgg
     2281 acggcagcac gccggcggcc gaacgcacgc gccgcgtcga ggcgttccag gaaggcgagg
     2341 gcgacctgtt cctgatcagc ctgaaggccg gcggcttcgg cctgaacctg acggtggccg
     2401 actatgtcgt catcgccgac ccgtggtgga acccggcggc ggaagaccag gccagcggcc
     2461 gcgcccaccg catcggccag cagcggccgg tgaccgtcta ccggctcgtc aacgccggca
     2521 cgctggagga gcgcatcctg acgctgcacc agaccaagcg cgagctggcc gacagcgtgc
     2581 tcgaaagcga cggccaggcg gccacgaccg tcccgctcgc gcgtgaactg ctgcagctga
     2641 tgcgcggcga cgcgatgccc gagtagcgac ccgccgccgg gcggacacgc tacgatgggg
     2701 ctcctcagcc agccagcgca ccgccttgac cgacgtcacc ggcatcgacc acatctacat
     2761 caccgtctcc gagctcgcac gctccgagcc gttctacgac cgcgtgctgc gcgacgcgct
     2821 gggctttcgc aagaacacgt tcacgctggg cggcgatccg cacatccagt actacaaccg
     2881 gctgttcggt ttcgtgctgc gccccgcgcg cgtgcagcgc gcgcacgagc cgtactcgcc
     2941 ggggctgcat cacttctgcc tgcgggtcga cagcgtcgag gacgtccaaa cggtcgcgcg
     3001 cgacctccag gccgccggca tcgccgccac cgacgcgcgt ttccaccccg agtattccga
     3061 cgactactgc gccaccttct tcgaggatcc ggacggtgtg agactcgagg ttaccaactg
     3121 gcgcctggag cgccgccaac gtgcggcaca ctgggacgat ccgcaaccct gatccgcgaa
     3181 cgccaggcac ccctgtccac cgtcgccgag cgccatgccg agttccccgc cgcccgatcg
     3241 cccgaaccgc ctgtccgagc tgcccggctc gcccctgcgc gccgtgctca cgggcgcggc
     3301 gatcgacatc tgtgggtcgc gggtcatcaa catcgtcgcg gtgacgatct atgcgatgca
     3361 actggtcacg ccgggcatga ccgacgagca ggccgccgac ctgctgcaga agacgccacc
     3421 cagcttcacc ctgctgctcg tgctcacggt gctgggcatg ctgatgtcgg tggccggcgg
     3481 ctacgcctgc gcgcgcgtgg tgctgcgcga cgagtggcgc accggcgcga tgctggccgc
     3541 cgtcgccgtc gtgttcggcc tgttcatcga tgacgccggc gtcagcgacc ttgcgctgct
     3601 gtacgtgctg tgcgacgtcg cgggcctgct gctgggcgtg aagtacggcg ctgaacgcaa
     3661 ccgccgcctc gaggcgcccg cgccatcgcc ggccgacgcg tccgccccat gaacggacgc
     3721 cgcgtcgcga tcggcctggg cgtcgtcgcg gcgttgggcg cgggcgcgtg gtgggccgtc
     3781 gcgatgttcg cgccgcaggc cttcatcgac atcgaccagg cgcagctgca gcagcggctc
     3841 gacggctact tcccgcagaa gcactgcacg atgatggtgg cctgcgtcac gctgagccaa
     3901 cccgtcgtca agctcaccga gggctcgccg cgcatcggac tgtcctccgg catcctggtc
     3961 acgctgggcc accgcgagat gccgggccag gtcaccttct ccggcgtgct gcgctacgtg
     4021 cgctaccagg gcgacttcta cctggacgac gtccagatcg agaacttcgc actcaccggc
     4081 gtctcgcccg acatggtgca ggtggtcaag caacgcggcc ccgacgccct gcgccgcgcc
     4141 ctgcagggcc acccgatcta cacgctgcgc ggcgactccg cccgccaggc gctggccaag
     4201 ctcgcggtgc gcaacgtgca ggtcgtcgac ggcaaggtga gggtgacgtt cctgagcttc
     4261 ggcgggtgag cgtcggcggc tcggctcgac agggtctcga gaggttggcg tggcatcccg
     4321 ctcacgcacg aaaacttgct gccaccgaaa ttccgtgcta gagtcacgga ctccgctcaa
     4381 ttgaccagcg gcaccgaatc aggagaggtg gcagagtggt cgattgtacc tgactcgaaa
     4441 tcaggcgtac cgcaaggtac cgtgggttcg aatcccaccc tctccgcca
//
LOCUS       DATCRO010000459         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_522417, whole genome shotgun sequence.
ACCESSION   DATCRO010000459 DATCRO010000000
VERSION     DATCRO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522417"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VIN75_12480"
     CDS             <1..192
                     /locus_tag="VIN75_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975031.1"
                     /translation="GQAQVDLFAAPAAADAAAAAAGPSRAEAALMALDPDQLAPREAL
                     EALYRLKSMTNTNEEVPSP"
     gene            189..1016
                     /locus_tag="VIN75_12485"
     CDS             189..1016
                     /locus_tag="VIN75_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEY8975032.1"
                     /translation="MTYCVGIRLDAGLVFMSDSRTNAGLDQISTFRKMIVYEKPGDRF
                     MVLLSAGNLSISQSVREILQVESVPNGDDEPITIWNARSMFDAARVLGAAVRRVYEND
                     GQALKNNGMDFVASFIFGGQIGNEAMRLFLVYSAGNFIEATRETCFFQIGESKYGKPI
                     LDRVLPPTTPLEEAAKCALVSMDSTLKSNLSVGLPLDLVVYEADKLQSDQIACIDEQN
                     PYFRMVRDSWGRRLREVFEAMDDPQWGDGASAATPLIVDAPRYEALKKIHNETEKVV"
     gene            1027..1872
                     /locus_tag="VIN75_12490"
     CDS             1027..1872
                     /locus_tag="VIN75_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975033.1"
                     /translation="MKTIVFAHANGFPAGTYEPLFDIWRRAGWRVEAPARFSHDPRYP
                     VTSNWPRLRDQLTDFIAANDLAGAMLVGHSLGGLVSLMAACKRPDLVGGLVMLDSPIV
                     TGWRAHSVHMAKRAGLIERVGPGAISRKRRHEWPSREAVHAHFAAKSSFARWDPRMLE
                     AYVKAGFEEVEDKVVLAVRREVETRIYNTLPHHVPALLRKHPPRCAVGFIAGTRSTEM
                     RQGGFASSHKLAGDRWRWIEGTHLYPMERPDDTAMLVLELLASMKPVTPRPRHAPAAA
                     PQPPA"
     gene            2082..>2462
                     /locus_tag="VIN75_12495"
     CDS             2082..>2462
                     /locus_tag="VIN75_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975034.1"
                     /translation="MRAHHKPHRQHPARPAAPDGDVALAEAEPGDATPPGEAAEPEAP
                     SRPVLSRCPPPLDEPQLIKPLDDSWRQWLAENALLGNSHDTMLARIKEAGIDPAQATG
                     YLAAIARDPLFRAGEKMRDRYTRFG"
BASE COUNT          372 a          923 c          840 g          327 t
ORIGIN      
        1 ggccaggcgc aggtcgacct gttcgccgcc cccgcggccg ccgatgccgc cgccgcggcg
       61 gccgggccgt cgcgcgccga ggccgcgctg atggcgctcg accccgacca gctggcgccg
      121 cgcgaggcgc tcgaggcact gtaccggctc aaatcgatga cgaacacgaa cgaggaggtc
      181 ccgtcgccat gacctactgt gtcggcatcc ggctcgacgc cgggctggtg ttcatgtccg
      241 actcgcgcac caacgcgggc ctggaccaga tcagcacctt ccgcaagatg atcgtctacg
      301 agaagccggg cgatcgcttc atggtgctgc tgtcggctgg caacctgtcg atctcgcagt
      361 cggtgcgcga gatcctgcag gtcgagtcgg tgcccaacgg ggacgacgag ccgatcacca
      421 tctggaacgc caggagcatg ttcgacgccg cccgcgtgct gggcgcggcc gtgcggcgcg
      481 tctacgagaa cgacggccag gcgctgaaga acaacggcat ggatttcgtc gccagcttca
      541 tcttcggcgg ccagatcggc aacgaggcga tgcggctgtt cctcgtgtac tcggcgggca
      601 acttcatcga ggccacgcgc gagacctgct tcttccagat cggcgaatcg aagtacggca
      661 agcccatcct cgaccgcgtg ctcccgccca cgaccccgct cgaggaggcg gccaagtgcg
      721 ccctggtgtc gatggactcg acgctcaagt ccaacctgtc ggtcggcctg ccgctcgacc
      781 tcgtcgtgta cgaggccgac aagctgcagt ccgaccagat cgcctgcatc gacgagcaga
      841 acccgtactt ccgcatggtg cgcgactcct ggggccggcg cctgcgcgag gtgttcgagg
      901 cgatggacga cccgcaatgg ggcgacggcg cctcggccgc cacgccgctg atcgtcgacg
      961 cgccgcgcta cgaggcgctc aagaagatcc acaacgagac cgagaaggtc gtctgaccgc
     1021 cccggcatga agacgatcgt cttcgcccac gcgaacggct tcccggcggg cacctacgaa
     1081 cccctgttcg acatctggcg ccgcgcgggc tggcgcgtcg aggcgccggc gcgcttcagc
     1141 cacgacccgc gctacccggt gacgagcaac tggccgcggc tgcgcgacca gctgaccgac
     1201 ttcatcgccg ccaacgacct cgccggcgcg atgctggtcg ggcactcgct gggcggcctg
     1261 gtcagcctga tggcggcgtg caagcggccc gacctggtcg gcggcctcgt gatgctcgat
     1321 tcgcccatcg tcaccggctg gcgcgcgcac agcgtgcaca tggccaagcg cgccgggctg
     1381 atcgagcgcg tcgggccggg cgccatctcg cgcaagcgcc gccacgaatg gccgtcgcgc
     1441 gaggccgtgc atgcgcactt cgcagccaag tcgtcgttcg cgcgctggga tccgcgcatg
     1501 ctcgaggcct acgtcaaggc cggcttcgag gaggtcgagg acaaggtcgt cctggccgtg
     1561 cggcgcgagg tcgagacccg catctacaac acgctgccgc accacgtgcc cgcgctgctg
     1621 cgcaagcacc cgccgcgctg cgccgtcggc ttcatcgccg gcacgcgctc gaccgagatg
     1681 cgccagggcg gcttcgccag ctcgcacaag ctggccggcg accgctggcg ctggatcgag
     1741 ggcacgcacc tctacccgat ggagcggccc gacgacaccg ccatgctggt gctggaactg
     1801 ctcgcctcga tgaagcccgt cacgccgcgt ccgcgccatg cgccggccgc cgcgccgcag
     1861 ccgccggcct gaacgcggcc gaggattcgt cgccgcgcgc gggcgccggc gcgagcgggc
     1921 gagggatcct cggcgcgccc cccgctcgcc gcggcattcg acgcgattcg cgcctccggc
     1981 gcgccggaaa ccccgggtcg ctccggcctg tggacgcatt gccggcgcct taagatcggg
     2041 cgtcgcccgg ccgcacgggt cgacagaccg ccggagactc catgcgcgcg catcacaagc
     2101 cccaccgcca gcatcccgcc aggcccgccg cccccgacgg cgacgtcgcg ctcgccgagg
     2161 ccgagccggg cgatgcgacc ccaccggggg aggccgccga gcccgaggcg ccgtcgcgcc
     2221 cggtgctgtc gcggtgcccg ccgccgctgg acgagccgca gctgatcaag ccgctggacg
     2281 acagctggcg gcaatggctg gccgagaacg cgttgctggg caattcgcac gacacgatgc
     2341 tcgcccgaat caaggaagcc ggcatcgacc cggcccaggc cacgggctac ctggccgcca
     2401 tcgcccgcga cccgttgttt cgcgccggcg agaagatgcg cgaccgctac acgcgcttcg
     2461 gc
//
LOCUS       DATCRO010000460        24081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_286131, whole genome shotgun sequence.
ACCESSION   DATCRO010000460 DATCRO010000000
VERSION     DATCRO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286131"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VIN75_12500"
     CDS             <1..192
                     /locus_tag="VIN75_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975035.1"
                     /translation="RELLERHAAALPDAAFTRLRAPEIGLTMVRARIGNQGDRSRRWT
                     SWRGAWTRRALSPAACSRA"
     gene            150..455
                     /locus_tag="VIN75_12505"
                     /pseudo
     CDS             150..455
                     /locus_tag="VIN75_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            467..1606
                     /locus_tag="VIN75_12510"
     CDS             467..1606
                     /locus_tag="VIN75_12510"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEY8975036.1"
                     /translation="MNDIVLSNARVVLGHETIHGHVVVRDGRIAAVASGPTVVPFAHD
                     LQGDWLLPGLVEMHTDNLERHVMPRPKVSFPMQAAVQAHDGEVAAAGITTVLDAIGVG
                     DPYDEGFRSRDQSELLRVLDRLEDAGSLRADHHIHVRAELPAPNARSLFEPFQRHHRL
                     RLISLMDHTPGQRQWTDIEHARTYYTGKKGWSNDKFAAEMRIAPERQARFAGPHRQWF
                     AEFARIHGVALATHDDTTVEHVDEALALGAAMSEFPTSLAAARHARASGLATIAGAPN
                     VVRGGSHSGNVAAMDLAREGVLDALSSDYVPASLLLAAWILKREAGFSPSEAVNVVSR
                     TPARACGFDDRGEIANGLRADLVRVREVDGHPVVREVWAQGRRVA"
     gene            1718..2116
                     /locus_tag="VIN75_12515"
     CDS             1718..2116
                     /locus_tag="VIN75_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975037.1"
                     /translation="MLKSILVVASSYVLSVVLVLCSDPLLTALFPGDFVSGHVPSDKA
                     LVASTALFVAISLSCAWLCARFSPRPTARRVLAFMILGELMGLGATIPHWSTDWPHWY
                     WLSWLISWPISCYVGWRIEQARSAPTAVAA"
     gene            complement(2127..2702)
                     /locus_tag="VIN75_12520"
     CDS             complement(2127..2702)
                     /locus_tag="VIN75_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8975038.1"
                     /translation="MHSDILDRIEHWFALHGATSYEGNRRESVSALAHALQCAQLAED
                     ACADSPLVAAALLHDVGHFVAIAEVGETADVDDVHELRAVALLVRDFPAAVVEPIRLH
                     VAAKRWLTAVDPAYLRGLSPASVHSLAQQGGPFQGDEIARFEGLPFAEDAVRLRRWDD
                     LAKTPGRETPGLGYYLALLDEVRLPARPLAS"
     gene            complement(2730..3497)
                     /gene="phnE"
                     /locus_tag="VIN75_12525"
     CDS             complement(2730..3497)
                     /gene="phnE"
                     /locus_tag="VIN75_12525"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY8975039.1"
                     /translation="MPPPPRASLQRLLFWGALLALLAGSWHGADVKPLELWRSGGNIG
                     EFLDGFFPPRITDWADDLKELLVTFEIALWGTALAVACSIPLGLLASANLTPWWIHHP
                     VRRVLDAFRAINEMVFALLFVTAVGLGPFSGVLALWVHTTGVLAKLFAEAVEASDPRP
                     AEGIRATGAHPLNVVFYGVVPQVVPLWVSFTLYRFESNVRSASVLGIVGAGGIGAVLW
                     DVIRGFEYGKTAFVLILLVAAVSLIDMASAQVRKRLI"
     gene            complement(3552..4526)
                     /gene="phnD"
                     /locus_tag="VIN75_12530"
     CDS             complement(3552..4526)
                     /gene="phnD"
                     /locus_tag="VIN75_12530"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8975040.1"
                     /translation="MIRRNALAVLVATSALWCTGAQAQTKEVNFGFISTESASNLKSA
                     WQPLLDDMQKTTGLKINAFFATDYAGVIEAMRFNKVQVAWMGNKSAMEAVDRSGGEIF
                     AKIVAKDGSEGYYGLMIVNKDSPIKSLDDVWKNHANLTLGFGDPNSTSGTAVPSFYAF
                     GPAKVDPLKDFKRTLRASHETNLLAVVNKQVDVATNNTENMQRFEQTHPEQARQVREI
                     WRSPLIASDPLVWRKDLDDGTKKQIRDFLLGYGKDAREKDILAKIEYSGFRASSNAQL
                     VPIRQIELARARAAAEADTTLGAADKAKKLADIDARLAELGKQVASAR"
     gene            complement(4557..5405)
                     /gene="phnC"
                     /locus_tag="VIN75_12535"
     CDS             complement(4557..5405)
                     /gene="phnC"
                     /locus_tag="VIN75_12535"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY8975041.1"
                     /translation="MDDLAIDVRQLHKTFGTAPALRDVSLQVRRGEMVALLGASGSGK
                     STLLRHLNGLHHADAGTTSSVNVLGRSVQHGGRIARDIRATRSRVAVVFQQFNLVERL
                     PVTVNVLAGALHRLPLWRGLLRRFPATEIARAHEAMSRVGIARLAWQRASTLSGGQQQ
                     RAAISRALVQGAEIILADEPIASLDPEASRKVMQSLADVNRDVGVTVLVSLHQVEFAF
                     SFCPRTIALHEGRVVFDGPTRALTPARLRELYGAQSDELLPTRTAPAVEPASEPVFAD
                     VALRVA"
     gene            5723..6247
                     /locus_tag="VIN75_12540"
     CDS             5723..6247
                     /locus_tag="VIN75_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975042.1"
                     /translation="MARTPSPLHDTSAPRRVSKPAVRAQAAAPTAEPVAAPAPATRKS
                     GAAETRAASPAKKAASAGKSDAAKVAPAKKSRAPSVAKAEPATAGAPASAPAKLAKAH
                     KPKLVRDSFTIPKDEYAVLDALKARALGLGQHVRKSELLRAGIQALHAMNDRALLAAV
                     GAVPTLKTGRPRKG"
     gene            complement(6349..6690)
                     /locus_tag="VIN75_12545"
     CDS             complement(6349..6690)
                     /locus_tag="VIN75_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975043.1"
                     /translation="MAFALDRMAFVVIKASWPPRSLAARPRRAGGLEGAAMTAPPSSF
                     RKARNRFPLAAIAHGLAVTLPTGEWFVEERGNVVMAFSDPLGETHVADVALVDFLDGL
                     ARREIVFMSWG"
     gene            7457..7918
                     /locus_tag="VIN75_12550"
     CDS             7457..7918
                     /locus_tag="VIN75_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8975044.1"
                     /translation="MSDDTSYRALVFRISGRLYSLPLAQVVETMRPLPVQRFHGAPDF
                     VLGAAIVRGQPVPVISAARLLGEAAVACRRWITIATGVRRVALEVDDVLGVRSIPAAD
                     DAMRQPLLEGAGLDRVATLGRLDGQLLRSLQGACLVPEDAWAAMGAEEAAA"
     gene            7960..9414
                     /locus_tag="VIN75_12555"
     CDS             7960..9414
                     /locus_tag="VIN75_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY8975045.1"
                     /translation="MPSAAPSPLERQRLRNVVAAVLGLQLDSLGERFVDEVLDRRAHA
                     LGVAIDTYLAGLEAGPAQAEIAALARELTVGETYFFRNIEQFDALRDWIDAWRATSAR
                     PIRVLSAGCASGEEPYSIAMVLHDASPEGAGAWTIRAVDANPAMIEKARRARYTRWAL
                     REMPLAMHQRWLRPDGAGVAVDDMIRDAVTFEERNLCAGDVDLWAPGAYDVIFCRNVL
                     IHLTRPAAEALAARLARSLVPGGALFLGHAETMRGLVDGFEAREACGTFWYERVVPAV
                     AGAAARHAGRTGVVADAGWTMPEPVGPAPASEPGRSAEAPAADALDRATELLRQERFD
                     EALAQVDALPEAAAAAPRALLLKGVLFAHRGDYAQASRIGTQLLASDERDADAYHLLA
                     LCRDAQGDRRGALYCEWRAVALDPDFALARMHLGLLARRTGDRDEMRRQFGRAEALLA
                     QEAASRLALFGGGFGRAALLAMCRAELVACGAAA"
     gene            9411..9983
                     /locus_tag="VIN75_12560"
     CDS             9411..9983
                     /locus_tag="VIN75_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8975046.1"
                     /translation="MTTGVPSQRADALRRAFDDAFAVEAGAGIAQADDLLAIRLRDDP
                     HLLRLADVGRLLRVGTLLRYPSSVPAWLGLAGVAGGVVPVYDLGVLLGYPPGDRPAWI
                     AVCGAEPVALAFDAFDGQVRHVREDATADAPAAASGTDVLRVAGEPRPVVDVAAVIER
                     LRSSVPDSPRAPTTASTSTPSPPGAADQEI"
     gene            9985..11448
                     /locus_tag="VIN75_12565"
     CDS             9985..11448
                     /locus_tag="VIN75_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY8975047.1"
                     /translation="MWTFGRKIALGFGISFVLLLITGMVAYRGVDALTQASYSVTHTH
                     QVIEHVVGMLSQLQEAEASQRGFLLTGEEAYLEPYNTAVGLVGKQSHELRTMTADNPT
                     QQRRLDEAEPLVQTRLAELAHNIQLRRDGGLDGGAKAVAAGTGKRTMDSLRAICQQME
                     AEERELLRQRAEEVEATSVHARQTVVVGTVLSLLFVCVAGFVIVRSLTTQIGSAVRHI
                     QSSSAELHASAAQQASSAKESSTSMAEITATISELMATSRQIAESAQEVARVAEQTAG
                     AARAGDGTVHVANESIASIRQQVDLVVDHTLELGRKSQQIGAVLDIVSELAEQTNILA
                     INATIEAAGAGDAGKRFSVVADEIRKLADRVSGSAKEIRALIDDVRSAVNTTVMATET
                     GSKAVDAGTREFAQVTAVFGQIAGMVVTAMQAAREIELSTKQQATAVEQVNLAIGSSA
                     QAARETEVSLGQTVQTASQLSTLSLGLMRLVQARSAA"
     gene            11463..13502
                     /locus_tag="VIN75_12570"
     CDS             11463..13502
                     /locus_tag="VIN75_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975048.1"
                     /translation="MAKDAYKYFRIESRELLDELGQGVLALERGASAREGVPALLRVA
                     HTLKGAARVVRQAGIAESAHRIEDTLVPWRDEIGAIPHDAIARVLAGLADIEARVAAL
                     PSADAPATSAPAAARPEEAVRSVRTDVAEMDALLAGLERTHGQVARLRQGRRETDAAR
                     VLVRSLRAPVRTGAGQAMAGAAPAALVDELQALVARLDDVVDTSAERLERELRQLRER
                     TERLRLIPVQTLFGRLERAARDVADSLGKQVSFEGVGGGVRLDASTLDAVQGALVQMV
                     RNAVAHGIEAPEVRLAAGKPRIGRITVEVLRQGRGVLFRCIDDGAGIDLEAVRRGAER
                     RGRLEAAAAPDAEALLHLLLQGGLSTSSVVTEVAGRGVGLDVAREAARRLGGTLSART
                     TAGGGTTIELAAPLPLAALDLLMVEAGTIVAGIPFDALSRAVRMGAADIVRSGARECV
                     LHEGRTVPYVALARLLAADQAGTPRDRVSVLVLEHAGQQAALGVDRLGEIASVVSRPL
                     PEFAPPCALAAGVATDIDGTPRLVLDVQALVRAAADAQAPATVERALPAPVLVVDDSL
                     TTRMLETSILESAGYVVDAAASAEEGLERAKRRRYALFLVDVEMPGMDGFGFIERTRA
                     DPALRDVPAMLVSSRSSLADRQRGAAVGAQGYIVKGEFAQDEFLARVRQLVGTTT"
     gene            13499..14578
                     /gene="cheB"
                     /locus_tag="VIN75_12575"
     CDS             13499..14578
                     /gene="cheB"
                     /locus_tag="VIN75_12575"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY8975049.1"
                     /translation="MRHAARTKLRVLVVEDSLTVRRRLCETLSAEPDIEVVGEAEDGR
                     RAIELCQALRPDVATIDMMLPLMTGVAATEYIMAHCPTPILIVSASTNRGELFKTYDA
                     LAAGAVDVLEKPRGDEPEGQWERRFVDAVRMVARIRVITHLRARYARPAPPPAASPTA
                     SGQNGRGCRIIALGASTGGPGAIVEVLRGLAPRVRAPVLLVLHINEPFGAAIADWLDA
                     QTTRRVIVARHGQPLAELTGCVALAPAGQHLIVRGGMTQLTRGPELHSCRPSVDVLFD
                     SVAAECGPSAIGCLLTGMGRDGAAGLLAMRRAGAVTIAQDEETSIVYGMPREAALLGA
                     ASRILPLESIGAAISDLALAAEVAR"
     gene            14575..17442
                     /locus_tag="VIN75_12580"
     CDS             14575..17442
                     /locus_tag="VIN75_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975050.1"
                     /translation="MKLSVVIVDDSLTVRMDLQEAFEDSGFAVRACANARQAREAFAA
                     GSVDVAVLDVQLPDGDGVELLRELRAMPNGTGAIVLMLSSASEVAQRIRGLRTGADEY
                     VGKPYDTQYVIAKTRELLRQRKGGHAPARATVLLVDDSATFRHAMREALEDAGYDVTT
                     AATGEEGLRLAAELLPSAIIVDGVLPGIDGMTVVRRVRLDAALRGVPCLLLTGSHELS
                     VELQALDAGADAFVHKDEEIPVILARLGAALRATERRAGEGPSSLLAPKKVLAVDDSA
                     TYLQELADVLRGEGYDVVSAHSGEQALELLSVQAVDCILMDLQMPGLDGRETCLRIKS
                     APGVRDIPLIMLTALEDRDAMLAGLGAGADDYIQKSSEFEVIKARVRAQLRRKQFEDE
                     NRRIREDLLHEASETRAARELAETRAGLVEQLERKSRELELATQAKSRFLAMMSHEIR
                     TPLNAVIGMAGLLGKSSLTDQQREYADIIRTSGDHLLAVINDILDFSSLEFGGLALES
                     RPMAVATVVEESLDLVAGAARAKDIELTYELAPGVPRTVLGDAGRIRQILVNYLSNAV
                     KFTAHGEVVVTIDSHLRADGRHELHGAVRDTGKGIPKDRFDRLFHSFSQVDASVRREF
                     GGTGLGLAICKRLAELMEGRVWVESVADRGSTFHFSFVAAIPEQTSSAEPEPMRKPLS
                     GVRAWIVDDNDANRHILRRQLQDWGLDIRDTALPEDALAWAQRGDPCDLAVLDFHMPV
                     MDGVQLARALNDLRGSALKQVLLTSGLPIPDDQARAAGLLAQLSKPVKHRPLLNTVLK
                     LFERRVIANAARPAAGPSAAPQAAPGAPAGGGLRILIAEDNAINVYLLKILLEQSGYG
                     ADVVGNGQEAIDAVRSKCYDVVFMDVQMPVMDGTEATRCIVDEHPDGDRPRIIALTAG
                     VMADEVQACRDAGVDDFLAKPIDPAKLAASLARCFRRDGGT"
     gene            complement(17635..19047)
                     /locus_tag="VIN75_12585"
     CDS             complement(17635..19047)
                     /locus_tag="VIN75_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8975051.1"
                     /translation="MKRYEALAQEIAESIAHGQLRPGERVPSVREASRTRGVSPSTVF
                     EAYYLLEARGLIEARPRSGYFVSHSVNRQQEPESSRPATQERTVEVSELVFQVLGHAR
                     NRALVPMGSAFPSPLLFPLPKLALATGKALRRLDPWRTVEDLSPGSAALRRQIGLRYL
                     GMGCGVDTEELVITNGAMEALNLCLESVTRPGDLVAVESPTFYAALQALERHSLRAVE
                     IATHPRTGIDVAALAAVMERHPVKACWLMPNFQNPLGSLMPDDAKQALVELLARRAVP
                     LIEDDVYGELYFGARKPRPAKTWDRAGWVMHCSSFSKTLAPGYRVGWVAAGRYAGEVA
                     QRKLMSSIAAPLPSQEGLSEYLEHGGYDRHLRQLRATLAHHRHTTLRGIAKHFPPGTR
                     ATQPEGGYFIWVELPPNIDALELHRLALSQGISLAPGHLFSADHRFKHHVRINFGHPG
                     HKNFNAALKTVGTIARALAA"
     gene            19243..19527
                     /locus_tag="VIN75_12590"
     CDS             19243..19527
                     /locus_tag="VIN75_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975052.1"
                     /translation="MQDPTRLEPLSRLPMQRIEKIMDWIPVVFIAFKLLVLGTAMFLS
                     IKSHHDKQKEEQEEERQRQARASGTRSASQSGQVQQHVEPSQCARQGGPG"
     gene            19919..22087
                     /locus_tag="VIN75_12595"
     CDS             19919..22087
                     /locus_tag="VIN75_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975053.1"
                     /translation="MNIRVRLMLLVFAVWLPAALGFGLLARSIYLRETASAREHVQEY
                     GRTLNLLVERELDKRATMARTLGASAALRRGDLRAFYDEAAAASRDTASWVILVTPTT
                     EVLNTHMPFSADLAIARPAGAPMISTDATDVFFAPAGPVRRAPVVALFRAPLGDLPPR
                     YNVGVALEPAAIQALLAQQAAPDGALVSVIDDEQLVMARTRDPGRWLGQPAGALIKQR
                     ARTGVEGFLDSVTLDGVSSLTWTSAPNAYRWNVVVATPKSALGAKARELTLQAVAAAA
                     ALLMIGLALVLAASRRISGPVRALHEAAAMLRADQVPLRLATGVREVDDISAVLHDAG
                     QHALEATRTLEQRVADAVLQAEDAQAKLLEGRKHEAIGRLTGGLAHDFNNLLQTISTA
                     LQVFERTRDAPPHPRTLDAALRATHRAAQLVRQMLTFGRTRPQQLAAVAFGDFLLGAR
                     ELVQKAVGQGVQLRADVDPALRHLHVDPSQLELALLNLIFNARDAMPDGGHVTVSARN
                     IEGQRAVVAVTDTGLGMDEQTLARATEPYFTTKPVGRGSGLGLAQVRGFALQSGGDIR
                     LSSTPGKGTTVEMHLPTVDAPPPAQAAPARASSGRPLRVLMAEDDLLVSSVVVPALEA
                     AGHAVTLSATADEAVERLRGGAAFDVVFTDVVMPGRMSGMDLAAWCREQRPAQPVVVA
                     SGYSAQALASVEVLNKPYDVNALLEALDRAAAGRLQEAAR"
     gene            22084..22659
                     /locus_tag="VIN75_12600"
     CDS             22084..22659
                     /locus_tag="VIN75_12600"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY8975054.1"
                     /translation="MSGGRRPGPSRRRVLRAVAAALGGLCLRARAQPPVRQEAVEAAY
                     LHKLPGFVEWPAQAFASAASPIVVGVAGDSPVLEELARIAKGRLVVGRGVEAHAIDAP
                     ADVPRELHVLFIGADAARSARALVEAARARHALIVTDLPDGLADGAAVQFVQVDGRLR
                     FEISLAGARRCGLKLSSQLMGVAWKVMEDGQ"
     gene            22656..>24081
                     /locus_tag="VIN75_12605"
     CDS             22656..>24081
                     /locus_tag="VIN75_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975055.1"
                     /translation="MTTPALRARLTRAFLLTIAAALLLDAVLVLGFEFQRFRSEAIDN
                     LHTQQSILARAVTPSLVFNDPDAAAQQLASLAENPRVRMAEVFGANGRPFARYTASKD
                     DAARLPASNPLPGLGSNFQGDDVEIAYDIFHDGERIGTLYLRARSDVARRLAGYAVIQ
                     ALVMAVSLGLALSVFGRVQRRITQPISRVARVAQEVVERRDWRLRAPSADTREIEALV
                     DAFNRMLAEMGESSTQLRQEIGERERAEDGLRAADRRKDEFLATLAHELRNPLAPMLN
                     AVALMRRGDLAPAVRDKALSILERQLRHVVRLIDDLLDVSRITTGKLSLHMEIVDLHA
                     VIRPTMELAETTAHDRQLQLTSAVPDAPCWVVGDTARLQQVFSNLLGNACRYTPPGGR
                     IEVTARCEDGFAIVEVADTGVGIDPQMHERVFELFEQGDKSLERGNAGLGIGLTLARQ
                     LVLLHGGTIGVSSRGIGHGSTFRVR"
BASE COUNT         3536 a         8347 c         8713 g         3485 t
ORIGIN      
        1 cgcgagctgc tcgagcgcca tgccgccgcg ctgcccgacg ccgcgttcac gcggctgcgc
       61 gcgccggaga tcggcctgac gatggtacgc gcccgcatcg gcaaccaggg cgaccgctcg
      121 aggcgctgga cgtcgtggcg cggcgcatgg acgcggcgcg cgctgtcgcc cgcggcctgt
      181 tctcgcgcct gaacctgccg cccgcgctgt gggacctgcc gccggcgacg ttctcgggcg
      241 gcgagcagca acgcgtcaac atcgcgcggg gcttcgtgca gccggcgcgc ctgctgctgc
      301 tggacgagcc caccgcttcg ctggacacgg ccaaccgccg cgtcgtcatc gagctcatcc
      361 acgaggccaa gacgcagggc gcggccgtcg tgggcatctt ccacgacgag gaagtgcgcg
      421 acgccgtcgc ggatcgccgc gtcgaactcg gttgaaggcc ttcgccatga acgacatcgt
      481 cctcagcaac gcccgcgtcg tcctcggaca cgagacgatc cacggccacg tcgtggtgcg
      541 tgacggacgc atcgccgcgg tggccagcgg cccgacggtc gttccgttcg cgcacgacct
      601 gcaaggcgac tggctcctgc ccggcctggt ggagatgcat accgacaatc tcgagcgcca
      661 tgtgatgccg cgccccaagg tgagcttccc gatgcaggcc gcggtgcagg cgcacgacgg
      721 cgaggtggcg gccgcgggca tcaccaccgt actcgacgcc atcggcgtgg gcgacccgta
      781 cgacgaagga ttccgctcgc gcgaccagag cgagctgctg cgcgtgctcg accgcctgga
      841 ggacgccggt tcgctgcgcg ccgaccacca catccacgtg cgcgccgagt tgccggcgcc
      901 caatgcgcgt tcgctgttcg agccgttcca gcgccaccac cggctgcggc tgatctcgct
      961 gatggaccac acgcccggcc agcgtcaatg gacggacatc gagcacgccc gcacgtacta
     1021 cacgggcaag aagggctgga gcaacgacaa gttcgccgcc gagatgcgga tcgcgccgga
     1081 gcgccaggcg cggttcgcgg ggccgcatcg ccagtggttc gccgagttcg cgcgcatcca
     1141 cggcgtggcg ctggccacgc atgacgacac gacggtcgaa catgtggacg aggccctggc
     1201 gctcggcgcc gcgatgagcg agttccccac cagcctcgcg gccgcgcgcc acgcgcgcgc
     1261 gagcggcctc gcgacgatcg ccggcgcgcc caacgtcgtg cgcggcggct cgcactcggg
     1321 caacgtggcc gcgatggacc tggcgcgcga aggcgtgctg gacgcgctgt cgtccgacta
     1381 cgtcccggcc agcctgctcc tggccgcgtg gatcctgaag cgcgaggccg gcttctcgcc
     1441 cagcgaggcc gtcaacgtcg tcagccgcac ccccgcgcgg gcctgcggct tcgacgaccg
     1501 cggggagatc gccaacggcc tgcgcgccga cctcgtgcgc gtgcgcgagg tcgacgggca
     1561 tccggtggtg cgcgaagtct gggcgcaggg gcggcgcgtg gcctgagcgt ccacggcaac
     1621 ttcgctccac gccggctcgg gtcaacacgg attcgcgcgg cgccgccaac ggccaagatg
     1681 cacgggagga accagcccgc catcccgagg aggccgcatg ctcaagtcga ttctcgtcgt
     1741 cgccagcagc tacgtgctgt cggtcgtgct ggtgctgtgc agcgatccgc tgctcacggc
     1801 cctgtttccg ggcgacttcg tcagcggaca tgtcccctcc gacaaggcgc tggtcgccag
     1861 cacggcgctg ttcgtcgcga tctcgctgtc gtgtgcctgg ctctgcgccc gcttctcgcc
     1921 gcgaccgacg gccaggcgcg ttctcgcgtt catgatcctc ggcgagctga tgggcctggg
     1981 ggcgacgatc ccgcactgga gcaccgactg gccgcactgg tactggctgt cgtggctgat
     2041 ctcctggccg atcagctgct acgtcggctg gcggatcgag caggcgcgct cggcaccgac
     2101 ggccgtcgcc gcctgaacct gcgcgctcag gacgcgagcg gccgcgcggg caggcggact
     2161 tcgtccagca gcgccaggta gtagcccagc ccgggcgtct cgcgcccggg tgtcttggcc
     2221 aggtcgtccc agcgccgcag ccgtaccgcg tcctcggcga agggcagccc ctcgaaacgg
     2281 gcgatctcgt cgccctggaa cgggccgccc tgctgcgcca gcgagtgcac cgacgccggc
     2341 gacaggccgc gcaggtacgc gggatcgacc gccgtcagcc agcgcttggc cgccacgtgc
     2401 agtcggatcg gctcgacgac ggcggccggg aaatcgcgca ccagcaaggc gacggcgcgc
     2461 agctcgtgca cgtcgtcgac atcggccgtc tcgccgacct cggcgatcgc gacgaaatgg
     2521 ccgacgtcgt gcagcagcgc cgcggccacc agcggcgaat cggcgcatgc atcctcggcc
     2581 agctgcgcgc actgcagtgc atgggcgagc gcgctgaccg actcgcggcg gttgccttcg
     2641 taggacgtcg cgccgtggag cgcgaaccag tgttcgatcc ggtcgaggat gtcggagtgc
     2701 atggatcgcc aggcggaggt tgtcggcgtt cagatcaggc gcttgcgcac ctgggccgag
     2761 gccatgtcga tcagggacac cgccgcgacc agcagaatga ggacgaacgc cgtcttgccg
     2821 tactcgaagc cgcggatcac gtcccacagc accgcgccga tgccgcctgc gccgacgatg
     2881 cccagcaccg acgccgacct cacgttcgac tcgaagcggt acagcgtgaa cgacacccac
     2941 agcggcacca cctgcggcac cacgccgtag aacacgacat tgagcggatg cgcccccgtc
     3001 gcgcggatgc cctcggccgg ccgcgggtcg ctggcctcga cggcttcggc aaacagcttg
     3061 gccaggacgc ccgtggtatg cacccacagg gcgagcacgc cggagaacgg ccccaggccg
     3121 accgctgtga cgaacagcag cgcgaacacc atctcgttga tcgcgcggaa cgcatcgagc
     3181 acgcggcgta cggggtgatg tatccaccac ggcgtcaggt tggcagacgc aagcaggccc
     3241 agcgggatgg agcaggccac cgcgagcgcg gtgccccaca aggcgatctc gaacgtgacc
     3301 agcagctcct tgaggtcatc cgcccagtcc gtgatgcgcg gcgggaagaa gccgtcgagg
     3361 aactcgccga tgttgccgcc ggagcgccag agctccagcg gcttcacgtc ggcgccgtgc
     3421 cagctgccgg ccagcaacgc caggagcgcg ccccagaaca acaggcgctg cagcgacgcg
     3481 cgcggcggcg gcgggatgga gcctgcgggg acggcggcca aggtctggct cacggcggcg
     3541 tcagcggggg atcacctggc gctggcgacc tgcttgccga gctcggcgag cctggcatcg
     3601 atgtcggcca gcttcttggc cttgtcggcg gcgcccagcg tggtgtcggc ctcggcggcg
     3661 gcgcgcgcgc gggccagctc gatctggcgg atcggcacca gttgcgcgtt ggatgaggca
     3721 cggaagccgc tgtactcgat cttggcgagg atgtccttct cccgcgcgtc cttgccgtag
     3781 cccagaagga agtcgcggat ctgcttcttg gtgccgtcgt ccagatcctt gcgccacacc
     3841 agcgggtcgc tcgcgatcag cggcgagcgc cagatctcgc gcacctgccg ggcctgctcc
     3901 ggatgcgtct gctcgaagcg ctgcatgttc tcggtgttgt tggtggcgac gtcgacctgc
     3961 ttgttgacga cggccagcag gttggtctcg tggctggcgc gcagcgtgcg cttgaagtcc
     4021 ttcagcggat cgaccttggc cgggccgaac gcgtagaacg acggcacggc ggtgccgctg
     4081 gtggagttcg ggtcgccgaa gcccagcgtc aggttcgcat ggttcttcca cacgtcgtcc
     4141 agcgacttga tggggctgtc cttgttgacg atcatcaggc cgtagtagcc ctcgctgccg
     4201 tccttggcca cgatcttggc gaagatctcg ccgcccgagc gatccacggc ctccatcgcc
     4261 gacttgttgc ccatccacgc gacctgcacc ttgttgaagc gcatcgcctc gatcacgccc
     4321 gcgtagtccg tggcgaagaa ggcgttgatc ttcaggccgg tcgtcttctg catgtcgtcc
     4381 agcaacggct gccaggcgct cttgaggttg gaggcggact cggtcgagat gaatccgaag
     4441 ttcacctcct tggtctgcgc ctgtgcgccg gtgcaccaca gcgcggaagt ggcgacgagc
     4501 acggccaggg cgttgcgacg gatcatggaa agctcctcgg gttggggaaa cggatttcag
     4561 gcgacgcgca gggcaacgtc ggcaaacacg ggttcggagg cgggctcgac ggccggcgcg
     4621 gtgcgcgtcg gcagcaactc gtcgctctgc gcgccgtaca gctcgcgcag gcgcgccggc
     4681 gtcagcgcgc gcgtggggcc gtcgaacacg acgcggccct cgtgcagcgc gatcgtgcgc
     4741 gggcagaacg agaaggcgaa ctccacctgg tgcagcgaca ccagcaccgt cacgcccacg
     4801 tcgcggttga cgtcggccag cgactgcatc accttgcggc tggcctcggg atcgagcgac
     4861 gcgatgggct cgtcggcgag gatgatctcc gcgccttgca ccagggcgcg cgaaatcgcg
     4921 gcacgctgct gctggccgcc cgacaacgtg gacgcgcgct gccacgcgag ccgcgcgatg
     4981 cccacgcgcg acatcgcctc atgggcccgc gcgatctccg tcgccgggaa gcgccgcagc
     5041 agcccgcgcc acagcggcag gcgatgcagc gcgccggcca gcacgttgac cgtcaccggc
     5101 aggcgctcga ccaggttgaa ctgctggaag acgacggcca cgcgcgaacg cgtggcgcgg
     5161 atgtcgcgcg cgatgcggcc accgtgctga acggaacgcc ccagcacgtt gacactggac
     5221 gtcgttcccg cgtcggcgtg gtgcaggccg ttgagatggc gcagcagcgt cgacttgccc
     5281 gagcccgagg cacccagcag tgccaccatc tcgccacggc gcacctgcag cgacacgtcg
     5341 cgcaacgcgg gcgcggtgcc gaaggtcttg tgcagctggc gtacgtcgat cgcgaggtcg
     5401 tccatgaggg ggctccggtg cacgcagcat cgctgcgccg ggagcaatca tctagacgac
     5461 gtgtgaccga tccgtgaacg gcgcatgaca cgccgatttc ggcgcacgag cagatccagc
     5521 gtggctgggg tggtcgtggc caacgcgaca ctgcccgccg caccgctctc ccgccgttgg
     5581 gtcagcagca aaggcgagtg cggttcagga gcccattcgc cggtcgccgc tcctcggctt
     5641 cggtctgccg gtcgtgtcgc caggcggcca aaggtcccgg tcaagtaaac tgtataaacc
     5701 gtttataccg aaaggacgac cgatggcaag gacacccagc ccgctgcatg acacgtccgc
     5761 gcccaggcgc gtgagcaagc ccgctgtgag ggcgcaggcc gctgcaccca cggcggagcc
     5821 ggtggctgcc ccggcgccgg ccacgcgcaa gtcgggcgcg gccgagacca gggccgcgtc
     5881 gcccgcgaag aaggcggcgt cggcgggcaa atccgatgcg gccaaggtcg cgccggcgaa
     5941 gaagtcgagg gcaccgtcgg tggccaaggc ggagccggcg accgccggcg ccccggcgtc
     6001 ggcaccggcc aagctcgcga aagcgcacaa gcccaagctg gtgcgcgaca gcttcacgat
     6061 cccgaaggac gagtacgccg tgctcgacgc gctcaaggcg cgcgcgctgg gcctgggcca
     6121 gcacgtgcgc aagtccgagt tgctgcgggc cggcatccag gcgttgcacg cgatgaacga
     6181 tcgcgccctg ctggccgcgg tcggcgccgt tcccacgttg aagacgggcc ggcccaggaa
     6241 gggctgagcg gcggcggcgc gaagccgtca gcgcgggccg attcagcgga ctgcttcgac
     6301 gacgagcatt cgtcggccga ggtcttcatc cgcgaccgcg caacgggctc agccccagga
     6361 catgaacacg atctcccggc gtgcgaggcc gtccaggaaa tcgacgagcg cgacgtcggc
     6421 gacatgcgtc tcgcccaacg gatcgctgaa agccatgacc acgtttccgc gctcctcgac
     6481 gaaccactcg cccgtcggca acgtgacggc caggccatgc gcgatcgcag ccaacggaaa
     6541 ccggttgcgg gctttcctga acgaggatgg aggagcggtc atggcggcgc cttcgagtcc
     6601 gcctgcgcga cggggccggg ccgccagact gcggggcggc catgacgcct tgatgacgac
     6661 gaaggccatc cgatccaggg cgaatgccat gtcgagttcc agctccggtt cgcctcgttg
     6721 ttccgtgagg gtggccgctg atcctcccct gcgacgaagt cggaacgtgc cgcagctcgg
     6781 tcaggtcgag ccctgtcgcg gcctccaggt tgcggcgccc cgcgggatcg atgatcgagc
     6841 gttgtcttcc acgggccgca gcgtcgccgg agtcgttgat tcagcgatag gggcatgggt
     6901 atctagcgag tcccttgaca cgctcgccat gcctgccttg ctccgaacct cgagccagag
     6961 gccgcaggcc cgggtggcgg gcccgactct tgccgatcac catccgggta ccgatcgatc
     7021 gacgagcggc gtccttggaa gtcaccggca ccgacccatc gggtgacacc gcgtagcgta
     7081 ccgaatccgg gcgagatcga tgccgggcgg ccttcgattc atgcgagtcc agaaggggaa
     7141 cgacgtagct ccgagtcttc gatgtcgggt atgcgcgaag aatctcgtaa cgcgcgcgcg
     7201 tgctgacgta cgcgagcgcc aaaggcttgc aaggcgtggc tgcggactcg atacccagcc
     7261 ggctcgtcgc aggaaaagtg agtgcggcac ggggtattcg tgcttgtgcg gcttgcgtga
     7321 atcgcgcgtc gcgcgctaac ctagcgcgac gggaatggac aggcaggcct gtcgattcca
     7381 ggctgtgccg gcacggtcgc tggagtcgga cgattcgtcg acggtcgtcc ggccgggctt
     7441 agggagtaga gagaacgtga gcgacgacac gtcatatcgc gcactggtct tccgcatttc
     7501 ggggcgcttg tattcgctgc cgcttgccca ggtggtcgag acgatgcgcc cgctgccggt
     7561 gcagcgattc cacggcgcgc ccgacttcgt gctcggcgca gccatcgtgc gagggcagcc
     7621 ggtgccggtc atctcggccg ctcgcctgct cggcgaggcg gccgtggcgt gccgccgctg
     7681 gatcaccatc gcgacgggcg tgcgacgggt cgcgctggag gtcgacgatg tgctgggcgt
     7741 ccgctcgata ccagccgcag acgatgcaat gcggcagccg ttgctcgaag gagccgggct
     7801 cgatcgcgtt gcgacgctcg gccggctcga cggccagctg ctgaggtctt tgcagggcgc
     7861 ctgcctcgtt ccagaggatg cctgggcggc gatgggcgcc gaggaggccg cggcgtgacc
     7921 gtgcgcaggc tcctgtcgcg gggggggcgg gccgccgcga tgccttccgc cgcgccgtcg
     7981 cccctggagc gccaacgcct tcgcaacgtc gtcgcggcgg tgctgggcct gcagctcgac
     8041 agcctcgggg agcgcttcgt cgacgaggtg ctggatcggc gcgcccatgc gctgggcgtt
     8101 gcgatcgaca cctacctcgc cggtctcgag gccgggccgg cgcaggccga gatcgcggcg
     8161 ctggcgcggg agctgaccgt cggcgagacc tacttcttcc gcaacatcga gcagttcgat
     8221 gcgctgcgcg actggatcga tgcctggcgc gcgacgagcg cgcggccgat ccgcgtgctg
     8281 tcggccggat gcgcctccgg cgaggagccg tactcgatcg cgatggtgct gcacgatgcg
     8341 agccccgagg gtgccggtgc gtggacgatc cgcgccgtcg acgcgaatcc cgcgatgatc
     8401 gagaaggccc ggcgcgcacg ctacacccgc tgggcgctgc gcgagatgcc gctcgcgatg
     8461 caccagcgat ggctgcggcc ggacggcgcg ggcgtcgcgg tcgacgacat gatccgggat
     8521 gccgtcacgt tcgaggagcg caacctgtgc gccggggacg tcgacttgtg ggcgccgggc
     8581 gcctacgacg tcatcttctg tcgcaacgtc ctgatccacc tcacgcggcc ggcggccgag
     8641 gcgcttgccg cgcgcctggc acgcagtctc gtgccggggg gcgccctgtt cctcgggcac
     8701 gctgaaacga tgcgaggcct cgtcgacggc ttcgaggcac gcgaggcatg cggcaccttc
     8761 tggtacgagc gtgtcgtgcc agccgtggcc ggcgcggcgg cgcgccatgc cgggcgcacc
     8821 ggtgtcgtcg cggacgccgg ctggacgatg ccggaacctg tcgggccggc accggcatcc
     8881 gagccggggc gatctgccga ggcgccggcg gccgatgcgc tggaccgggc gaccgagttg
     8941 ctccggcagg agcgcttcga cgaggcgctc gcacaggtgg acgcgctgcc cgaggccgcg
     9001 gccgccgcac cgcgggcgct gttgctcaaa ggggtgctgt tcgcccatcg aggcgactat
     9061 gcgcaggcgt cgcgcatcgg cacgcagctg cttgcatccg acgaacgcga tgccgacgcc
     9121 taccacctgc tcgcgctgtg ccgcgacgcg cagggcgatc ggcgcggggc gctgtattgc
     9181 gaatggcgag ccgtcgcgct cgatccggac ttcgcgctgg cacgcatgca cctgggtctc
     9241 ctggcgcgcc ggactggcga ccgcgacgag atgcggcgcc agttcggccg cgccgaggcg
     9301 ctgctcgccc aggaggccgc ctcccggctc gcgttgttcg gaggcggatt cgggcgcgcg
     9361 gccttgctcg cgatgtgccg cgccgagctg gtcgcctgcg gggcagcggc atgacgacgg
     9421 gcgtgccgtc ccagcgcgcc gatgcgttgc gccgcgcttt cgacgacgcc ttcgccgtcg
     9481 aggccggggc aggcatcgcg caggccgacg acctgctcgc gatccggctg cgcgacgatc
     9541 cgcacctgct gcgcctggcc gacgtcggcc gcttgctgcg cgtggggacg ctgctgcgct
     9601 acccgagttc agtgccggcg tggctggggc tggccggcgt ggcaggtgga gtcgtgccgg
     9661 tgtacgacct gggtgttctc ctggggtatc cgccgggaga ccggcccgcc tggatcgccg
     9721 tctgtggcgc cgagcctgtc gcattggcat tcgacgcgtt cgacggccag gtgcgccatg
     9781 tgcgtgaaga cgcgacggcc gacgccccgg ccgccgcatc cgggacagac gtcctgcgcg
     9841 tggccggcga gccgcgcccc gtcgtcgacg tcgctgcagt gatcgagcgc ctgcggtctt
     9901 cggttcccga ttcgccgcgt gcgccgacaa ctgcttcaac atcaacgcca tcgcccccgg
     9961 gggcggcaga ccaggagatc tgagatgtgg acattcggac ggaagatcgc cctcggattc
    10021 gggatctcct tcgtgctcct gctgatcacc ggcatggtgg cctatcgcgg cgtcgacgcg
    10081 ctcacccagg ccagctattc cgtgacccat acgcaccagg tgatcgagca cgtggtgggg
    10141 atgctcagcc agctccagga agccgaggcc agccagcgcg gcttcctgct gaccggcgag
    10201 gaggcctacc tcgagccgta caacaccgcg gtcggtctcg tcggcaagca atcgcacgag
    10261 ctgcgcacga tgaccgcgga caacccgacg cagcagagac ggctcgacga agccgagcct
    10321 ctcgtgcaga cccggctggc ggagctcgcg cacaacatcc agctgcgccg ggacggcggc
    10381 ctggacggcg gcgcgaaggc ggtggcggcg gggacgggca agcgcacgat ggactcgctg
    10441 cgcgcgatct gccagcagat ggaagccgag gagcgcgagc tgctgcgcca gcgcgccgag
    10501 gaggtcgagg ccacttccgt ccatgcgcgc cagaccgtcg tcgtcggaac cgtgctgagc
    10561 ctgctgttcg tttgcgtcgc cggcttcgtg atcgtgcgat cgctgacgac gcagatcgga
    10621 tcggccgtgc gccacatcca gagttcgtcc gcggagctgc atgcgtccgc ggcacagcag
    10681 gccagcagcg ccaaggagtc ctcgacctcc atggccgaga tcacggccac catcagcgag
    10741 ctcatggcga cctcgcgcca gatcgccgaa agcgcccagg aggtggcgcg cgtggccgag
    10801 cagacggccg gcgccgcgcg cgcgggcgac ggcaccgtgc acgtggccaa cgaatccatc
    10861 gcgagcatcc gccagcaggt ggacctcgtc gtggatcaca cgctggaact gggacgcaag
    10921 tcgcagcaga tcggcgcggt gctggacatc gtgtcggagc tggccgagca gacgaacatc
    10981 ctggcgatca acgcgaccat cgaggcggcg ggggcgggcg acgcgggcaa gcgcttctcg
    11041 gtcgtcgcgg atgaaattcg caagctggct gaccgcgtgt ccggctctgc caaggagatc
    11101 cgcgcgctca tcgacgatgt gcgcagcgcg gtcaatacga cggtgatggc gacggagacg
    11161 ggttccaagg cggtggatgc cggaacgcgc gagttcgccc aggtcaccgc tgtcttcggc
    11221 cagatcgcgg gcatggtcgt caccgcgatg caggcggcac gcgagatcga gctgtcgacc
    11281 aagcagcagg cgactgcggt ggaacaggtc aacctggcga tcggcagcag cgcccaggcg
    11341 gcgcgcgaga ccgaagtgag cctcgggcag acggtgcaga cggcctctca gctgtccacg
    11401 ttgtcgctcg ggctgatgcg ccttgtccag gcccggtcgg cggcctgaac gtcgtccgcg
    11461 ccatggccaa ggacgcctac aaatacttcc gcatcgagtc gcgggaactg ctcgacgagc
    11521 tcgggcaagg cgtgctcgcg ctcgaacgtg gcgccagcgc gcgcgaaggc gtgccggcgt
    11581 tgctgcgcgt cgcgcacacg ctcaagggcg cggcgcgcgt ggtgcggcag gccggcatcg
    11641 cggagtcggc ccaccgcatc gaggacacgc ttgtcccctg gcgcgacgag attggagcga
    11701 tcccgcacga cgccatcgcc cgggtgctcg caggcctggc ggacatcgag gccagggtcg
    11761 cggcgctgcc gtctgccgat gcgccggcga cctccgctcc cgcggcggcc cgccccgagg
    11821 aggcggtgcg cagcgtgagg acggacgtgg ccgagatgga cgcgctgctc gccggtctgg
    11881 agcgcacgca tgggcaggtc gcccggctgc ggcaagggcg ccgcgagacg gatgccgcac
    11941 gcgtcctcgt gcgcagcctg agggcgcctg tccggacagg cgccggacaa gcgatggccg
    12001 gggcggcgcc cgccgcgctg gtcgatgaac tgcaggcgct cgtcgcgcgc ctggacgacg
    12061 tcgtggacac ctcggccgag cgcctggagc gcgagctgcg gcaactgcgc gagcgcaccg
    12121 agcggttgcg gctgatcccg gtgcagaccc tgttcgggcg gctcgagcgc gctgcacgcg
    12181 acgttgccga ctcgctgggc aagcaggtct cgttcgaagg tgtcgggggc ggcgtccgcc
    12241 tggacgcctc cacgctggac gccgtccagg gggcgcttgt ccagatggtg cgcaacgcgg
    12301 tcgcccacgg catcgaggcc ccggaggtcc gcctcgccgc gggaaagccg cgaattgggc
    12361 gcatcacggt ggaagtcctg cggcagggac gtggcgtgct gtttcgctgc atcgacgatg
    12421 gcgccggcat cgacctcgag gcggtgcgcc gcggcgccga acgcaggggg cggctggagg
    12481 ccgctgccgc gccggacgcg gaggcgctgc tgcacctgct gctgcaaggc ggactgagca
    12541 cgtcgagcgt cgtcaccgaa gtagccggcc gcggcgtcgg cctcgacgtc gcacgcgagg
    12601 cggcccggcg attgggtgga acgctgtcgg cgcgcacgac ggcgggcggg gggaccacca
    12661 tcgagctggc ggcaccgctg ccgctcgcgg cactcgactt gctgatggtc gaggccggaa
    12721 cgatcgtggc gggcattccg ttcgatgcgc tgagccgcgc ggtgcgcatg ggggccgcgg
    12781 acatcgtgcg cagcggcgcg cgcgagtgcg tgctgcacga aggccgcacc gtgccctacg
    12841 tcgcgctggc gcgcctgctg gccgcggacc aggccggcac gccgcgcgat cgcgtgtcgg
    12901 tgcttgtgct ggaacatgcg ggccagcagg ccgcgctggg ggtcgaccgc ctgggggaga
    12961 tcgcctccgt ggtctcgcgg ccgctgcccg agttcgcgcc gccctgcgcg ctggcggcag
    13021 gcgtcgccac cgacatcgac ggcacgccgc ggctggtgct ggacgtgcag gcgctggtgc
    13081 gtgcggccgc ggatgcccag gcgcccgcca ccgtcgagcg cgcgctgccg gcgccggtgc
    13141 tggtggtcga cgattccttg acgacgcgca tgctggagac cagcatcctc gagtccgccg
    13201 gctacgtcgt cgacgcggcc gcctcggccg aggaaggcct ggagcgtgcg aagcgccggc
    13261 gctatgcctt gttcctcgta gacgtcgaga tgccgggcat ggacggcttc gggttcatcg
    13321 aacgtacgcg cgccgatccg gcgctgcgcg acgtgccggc gatgctggtc agctcgcgat
    13381 cgtcgctggc cgatcgccag cgcggcgcgg cggtcggggc gcagggctac atcgtcaagg
    13441 gcgaattcgc gcaggacgag ttcctcgcac gagtgcgcca gctcgtggga acgacgacat
    13501 gagacatgcg gccaggacga agctgcgcgt gctggtggtg gaggattcgc tcacggtgcg
    13561 gcgccggctc tgcgagacgc tctcggccga gcccgatatc gaggtcgtcg gcgaagccga
    13621 ggacggccgt cgtgccatcg agctgtgcca ggccctgcgg ccggacgtcg cgacgatcga
    13681 catgatgctg ccgctgatga cgggcgtggc ggccaccgag tacatcatgg cgcactgtcc
    13741 gacgcccatc ctcatcgtct ccgcgtcgac caaccgcggc gagctgttca agacctacga
    13801 cgcgctcgcg gccggcgccg tcgacgtgct ggagaagccg cgcggcgacg aacccgaggg
    13861 ccagtgggag cggcgcttcg tcgatgcggt gcgcatggtg gcgcgcatcc gcgtcatcac
    13921 gcacttgcgc gcccggtatg ccaggccggc gccgccgcca gctgcctcgc cgaccgcatc
    13981 gggccagaat ggacgcggct gccggatcat cgcgctcggt gcgtcgaccg gtggacccgg
    14041 ggcgatcgtc gaggtgctgc gcggcctggc gcccagggtc cgggcgccgg tgctgctggt
    14101 gctgcacatc aacgagccgt tcggtgcggc catcgccgac tggctggacg cccagacgac
    14161 gcgccgcgtg atcgtcgcgc gccacggcca gcccctcgcg gagctcaccg gctgcgtggc
    14221 gctggcgcct gccggacagc acctgatcgt gcgcggcggc atgacgcagc tgacgcgcgg
    14281 gccggagctg cactcgtgcc ggccgtccgt cgacgtgctg ttcgactcgg tcgccgccga
    14341 atgcgggccg tccgccatcg gctgcctgct cacgggcatg ggccgcgacg gtgcggcggg
    14401 cctgctggcc atgaggcggg ccggcgccgt caccatcgcg caggacgagg agacctcgat
    14461 cgtctacggc atgccccgcg aggccgcgct gctcggcgcc gcgtcgcgca tcctgccgct
    14521 cgagtcgatc ggcgcggcga tcagcgacct ggccctcgcc gcggaggtcg cgcgatgaag
    14581 ctgtcggtgg tcatcgtcga cgacagcctc actgtccgca tggacctgca ggaagcgttc
    14641 gaggactccg gattcgccgt gcgtgcctgc gccaacgcca ggcaggcgcg cgaggccttc
    14701 gcggcaggaa gcgtcgatgt cgcggtgctg gatgtccaac tgcccgatgg cgacggcgtc
    14761 gagctgctgc gcgaattgcg agccatgccc aatggcacgg gtgcgatcgt gctgatgctg
    14821 tcgagcgcgt cggaggtcgc ccagcgcatc cggggcttgc gcaccggcgc ggacgagtac
    14881 gtcggcaagc cctacgacac gcagtacgtg atcgccaaga cgcgcgagtt gctgcgccag
    14941 cgcaagggcg gccacgcacc cgcgcgcgcg accgtcctgc tggtcgatga cagcgcgacc
    15001 ttccggcatg ccatgcgcga ggcgctcgag gacgccggct acgacgtcac gaccgcggcc
    15061 acgggcgagg aagggctgcg gctggccgcg gagctgctgc ccagcgcgat catcgtcgac
    15121 ggcgtgctgc cgggcatcga cggcatgacg gtggtgcgcc gcgtgcggct cgacgcggcg
    15181 ctgcgcggcg tgccctgcct gttgctgacc ggctcgcacg agctgagcgt cgaactgcag
    15241 gcgctcgacg ccggggccga tgccttcgtc cacaaggacg aggagatccc ggtgatcctg
    15301 gcgcgcctgg gcgcggcgct ccgcgccacg gaacgccgcg ccggcgaggg gccgtcgagc
    15361 ctgctcgcgc cgaagaaggt gcttgccgtc gacgacagcg cgacctatct gcaggagctc
    15421 gccgacgtct tgcgcggcga ggggtacgac gtcgtctccg cgcactccgg cgagcaggcg
    15481 ctggagctgc tgtcggtgca ggcggtcgac tgcatcctga tggacctgca gatgccgggc
    15541 ctcgacggcc gcgagacctg cctgcgcatc aagagcgcgc ccggcgtccg cgacatcccg
    15601 ctgatcatgc tcaccgccct cgaggaccgc gacgcgatgc tggcgggcct gggcgcgggc
    15661 gcggacgact acatccagaa atcttcggag ttcgaggtca tcaaggcccg tgtccgggcg
    15721 cagctgcgac gcaagcagtt cgaggacgag aaccgccgca tccgcgagga cttgctgcac
    15781 gaggcctccg agacgcgcgc ggcgcgcgaa ctggccgaga cgcgcgcggg tctcgtcgag
    15841 caactcgaac gcaagagccg cgagctggag ctggccacgc aggccaagag ccgattcctg
    15901 gcgatgatga gccacgagat ccgcacgccc ttgaacgccg tcatcggcat ggccgggctg
    15961 ctgggcaagt cgtcgctcac cgaccagcaa cgcgaatacg ccgacatcat tcgcaccagc
    16021 ggcgatcacc tgctggcggt gatcaacgac atcctcgact tctccagcct cgagttcggt
    16081 ggcctcgcgc tggagagccg cccgatggcg gtggccacgg tcgtcgagga gtcgctcgat
    16141 ctcgtcgccg gcgcggcacg agccaaggac atcgagctga cctacgagct tgcccccggc
    16201 gtgccgcgga cggtgctcgg cgatgcgggg cgcatccgcc agatcctggt gaactacctg
    16261 tcgaacgccg tgaagttcac cgcgcacggc gaggtggtcg tcaccatcga cagccatctg
    16321 cgggccgacg gtcgacacga gctccacggt gcggtgcgag atacaggcaa gggtattccg
    16381 aaggaccgct tcgaccggct gttccattcg ttcagccagg tcgacgcgtc ggtgcgtcgc
    16441 gagttcggcg gcacgggcct cggcctcgcg atctgcaagc gcctggccga gctcatggag
    16501 ggacgcgtct gggtcgagag cgtggcggac cgcggctcga ccttccattt cagcttcgtg
    16561 gcggccatcc cggaacagac gtcatccgcg gagccggagc ccatgcgcaa gccgctgtcc
    16621 ggagtgcgcg cctggatcgt cgacgacaac gatgccaacc gccacatcct gcggcggcag
    16681 ctccaggact gggggctcga catccgcgac accgctttgc ctgaggacgc gctggcctgg
    16741 gcgcaacgag gcgacccgtg cgacctggcc gtgctggact tccacatgcc cgtcatggat
    16801 ggggtgcagc ttgcccgggc gctcaacgac ctgcgcggca gcgcgctcaa gcaggtgctc
    16861 ctgacttccg ggctgcccat ccccgacgac caggcgcgcg cggcgggcct gctggcgcag
    16921 ttgtccaagc ccgtcaagca tcgcccgttg ctcaacacgg tgctcaagct gttcgagcgg
    16981 cgcgtgatcg ccaacgcggc acgtccggcc gccggcccgt cggcggcgcc gcaagccgcg
    17041 cccggcgcgc ccgccggcgg cggcctgcgc atcctcatcg cggaggacaa cgcgatcaac
    17101 gtctacctcc tgaagatcct gctcgaacag tccggctacg gcgccgacgt cgtcggcaac
    17161 ggccaggagg cgatcgacgc cgtgcgcagc aagtgctacg acgtcgtctt catggacgtg
    17221 cagatgcccg tgatggacgg aaccgaagcg actcgctgca tcgtcgacga gcatccggac
    17281 ggcgaccgcc cgcgcatcat cgcgctcacg gcgggcgtga tggccgacga agtccaggcg
    17341 tgccgcgatg ccggcgtgga cgacttcctg gccaagccga tcgatccggc caagcttgcc
    17401 gcgtcgctgg cgcgctgctt ccggcgcgat ggcggcacct gacgggattc ggcccgtctc
    17461 cgctgcgcct cggcgcctgg caaagcgaca cgcctgaatc cgtatgatgc cgcgctcgag
    17521 gcactgatgc ccggtctgcg atgggtcggc ggccgcgggg atgccgcgct cgcggccggc
    17581 gcgcgctgta cgaagcgaca ttcaagccgt atgcggcagg cactcggctt gcgatcaggc
    17641 cgccagcgcc cgggcaatgg ttcccaccgt cttgagcgcc gcgttgaagt tcttgtggcc
    17701 gggatgcccg aagttgatcc gcacatggtg tttgaagcga tggtcggccg agaacagatg
    17761 gcccggcgcc aggctgatgc cctgcgacag ggccagccgg tggagttcca gcgcgtcgat
    17821 gttcggtggc agttccaccc agatgaagta gccgccttcg ggctgcgtgg cccgcgtgcc
    17881 gggcggaaag tgcttcgcga tgccgcgcag cgtcgtatgg cgatggtgcg ccagcgtcgc
    17941 ccgcaactga cgcagatgcc ggtcgtaacc gccatgttca aggtattcgc tcaagccctc
    18001 ctgcgagggc aagggcgcgg cgatggaact catcagcttg cgttgcgcaa cctcgccggc
    18061 gtaacggccc gctgccaccc agccgacgcg gtagcccggc gccagcgtct tggagaagga
    18121 actgcagtgc atgacccagc ccgcgcgatc ccacgtcttg gcgggcctgg gcttgcgggc
    18181 gccgaaatac agctcgccat agacgtcatc ttcgatcagc ggcaccgcgc gcctggcgag
    18241 cagttccacc agggcttgct tggcgtcgtc cggcatcagg ctgccaaggg gattctggaa
    18301 gttgggcatg agccaacatg ccttgaccgg atgccgttcc atgaccgcgg ccagcgcggc
    18361 cacgtcgatg cccgtgcgcg gatgggtggc gatctcgacg gcgcgcaggc tatggcgctc
    18421 cagcgcttgc agcgcggcgt agaaggtcgg agactcgacc gccaccaggt cgccgggccg
    18481 ggtgacgctt tccaggcaga ggttcagcgc ctccatcgcg ccgttggtga tgaccagttc
    18541 ctcggtgtcc acgccgcagc ccatgcccag atagcgcagg ccgatctgac ggcgcagcgc
    18601 cgcgctgccg ggcgaaagat cttcgaccgt gcgccatggg tccaaccgac gcagcgcctt
    18661 gcccgtggcg agtgcgagct tgggcaaggg aaacagcagc ggactgggga aggccgagcc
    18721 catcggcacg agcgcccgat tcctggcgtg gccaagtacc tggaagacca gctcggatac
    18781 ctcgacggtg cgctcctgcg tcgccggcct tgaactctcc ggctcctgct gccggttcac
    18841 cgagtggctg acgaagtagc cggaacgggg gcgggcctcg atcaggccgc gcgcttcgag
    18901 caggtagtac gcctcgaaga cggtggacgg gctcacgccc cgggtgcggc tcgcctcccg
    18961 caccgaaggc acgcgctcac ccggccgcaa ctggccgtgg gcgatggact cggcgatctc
    19021 ttgcgccagg gcttcatagc gcttcatggt gcctcgtcgt cgtgtgcaac tgacatgcac
    19081 ggaaagcgga tcgaacgcca ctgtactttc acgagaattt ccctggcaaa gcccccatgc
    19141 ccatcatctg atccgctgtt tttcgggccc atctgagtct gtgatcccgg cgaccgtttt
    19201 ggcacagtgg cggctgcaac aaacaggatc gtcgtgggat ccatgcagga tccaacccgg
    19261 cttgaaccat tgtccaggct gccgatgcag cgtatcgaga aaatcatgga ttggattccg
    19321 gtagtcttca ttgcattcaa gttgctcgtg ctgggcacgg ccatgttctt gtccatcaag
    19381 tctcatcacg acaagcagaa ggaagagcaa gaagaagaaa gacagaggca ggcgcgtgca
    19441 tccggaaccc gcagcgccag ccaatccggg caagttcaac aacacgttga accttcgcaa
    19501 tgcgcaaggc aaggtggccc gggctgatga gccgggggcg atcgccgtct gcccgggttt
    19561 ccgacatcga gacggctcga ctcggaacac ggttgcgcac tcctcggcca gcgactgccg
    19621 aaccggctgg tacctgagac agtccacgag cggctcgagc gcctcgatgg cgccggtcgg
    19681 cgacgcggag cggccggccg ccaatccgtg aacgacgcgc gcgggcgtct tgtcacgtcg
    19741 ataccctctg cagtcccggc gccggggctt caccgagcga acgcctacct cagtggcacg
    19801 cttcgtgctg ccagcacccc cagcccgcct gctcgaccgt tttgaccgac gagcgagcgc
    19861 gaggcagcgt ggcgggcgtc cgttcgtctg tacgatttac aaaaacgcaa aggtcctcgt
    19921 gaacatccgc gtccggctga tgctgctggt cttcgccgtc tggctgccgg cggcgctggg
    19981 cttcgggctg ctggcgcggt cgatctacct gcgcgagacg gcgtcggccc gcgagcatgt
    20041 gcaggagtac ggacggacgc tgaacctgct ggtcgagcgc gagctggaca agcgggcgac
    20101 gatggcgcgc acgctgggcg cgtcggcggc gctgcggcgg ggcgacctgc gcgccttcta
    20161 cgacgaggcg gccgcggcca gtcgcgacac ggcgagctgg gtgatcctcg tcacgccgac
    20221 caccgaggtg ctcaacacgc acatgccgtt ctcggccgac ctggccatcg cgcgtccggc
    20281 cggcgcgccc atgatctcga ccgacgcgac ggatgtcttc ttcgcgcccg ccggcccggt
    20341 caggcgcgcg cccgtcgtcg cgttgttccg cgcgccgctg ggcgacctgc cgccgcgcta
    20401 caacgtgggc gtcgcgctgg agccggcggc catccaggcg ctgctcgcgc agcaggcagc
    20461 acctgacggc gcgctcgtct ccgtgatcga tgacgagcag ctggtgatgg cgcgcacgcg
    20521 cgaccccggc cgttggctgg gccagccggc cggtgcgctg atcaagcagc gcgcgcgcac
    20581 cggtgtcgag ggcttcctcg actcggtcac gctcgacggc gtcagctcgc tcacctggac
    20641 cagcgcgccc aacgcctatc gctggaacgt cgtcgtcgcg acgcccaaga gcgcgctcgg
    20701 cgcgaaggcg cgcgagctca cgctgcaggc tgtcgctgcc gcggcggccc tgctgatgat
    20761 cggcctggcg ctcgtgctcg ccgcgtcgcg ccgcatcagt ggcccggtgc gcgcactgca
    20821 cgaggcggcc gccatgctgc gcgccgacca ggtcccgctg cgcctggcca ccggcgtgcg
    20881 cgaagtcgac gacatcagcg ccgtcctgca cgacgccggc caacatgcgc tggaggccac
    20941 gcgcacgctg gagcagcgcg tggccgacgc cgtgctgcag gccgaggacg cgcaggccaa
    21001 gctgctcgag ggccggaagc acgaggcgat cggacggctc accggcggcc tggcgcatga
    21061 cttcaacaac ctgctgcaga cgatcagcac ggcgctgcag gtgttcgagc gcacgaggga
    21121 cgcgccgccg catccgcgca cgctggacgc ggccctgcgc gcgacgcatc gcgccgcgca
    21181 gctcgtgcgg cagatgctca cgttcggccg cacgcgcccg cagcagctgg cggccgtcgc
    21241 gttcggcgac ttcctgctgg gggcgcgcga gctggtgcag aaggcggtcg ggcagggcgt
    21301 gcagctgcgc gcggacgtcg acccggcgct gcggcacctg cacgtcgatc cctcgcagct
    21361 ggagctggcg ctgctgaacc tcatcttcaa cgcgcgcgac gccatgcccg acggcggcca
    21421 cgtcaccgtc agcgcgcgca acatcgaggg ccagcgcgcg gtggtggccg tgacggatac
    21481 gggcctgggc atggacgagc agacgctggc gcgcgccacc gaaccctatt tcacgaccaa
    21541 gccggtgggg cgcggcagcg gcctcggact tgcccaggtt cgcgggttcg cgctgcagtc
    21601 cggcggcgac atccggctgt cgagtacgcc cggcaagggg acgaccgtgg agatgcacct
    21661 gccgaccgtc gacgcgccgc cacccgcgca ggcggcgccg gcgcgcgcgt ccagcggccg
    21721 gccgctgcgc gtgctgatgg cggaggacga cctgctcgtg tcgtcggtgg tggtgcccgc
    21781 gctggaggcg gcgggccatg ccgtcacgtt gtcggccacg gccgacgagg cggtcgagcg
    21841 cctgcgcggc ggcgcggcgt tcgacgtggt gttcacggat gtcgtgatgc ccggccgcat
    21901 gagcggcatg gacctggccg cgtggtgccg cgagcagcgg cccgcgcagc cggtggtcgt
    21961 ggcctccggc tactccgcgc aggcgctggc cagcgtcgaa gtgctcaaca agccctacga
    22021 cgtgaatgcg ctgctcgagg cgctggaccg cgccgcggcc gggcggctcc aggaggccgc
    22081 gcggtgagcg gcggccggcg gcccgggccg tcgcgccgcc gcgtgctgcg tgcggtcgca
    22141 gctgcgctcg gcggcctgtg cctgcgcgcg cgggcgcagc cgccggtgcg gcaggaggcg
    22201 gtcgaggcgg cctatctgca caagctgccg ggcttcgtcg agtggccggc gcaggcgttc
    22261 gcgtcggcgg cgtctccgat cgtcgtcggc gtggccgggg actcgccggt gctcgaggaa
    22321 ctggcgcgca tcgccaaggg ccggctggtg gtcggacgcg gcgtcgaggc gcacgccatc
    22381 gacgcgcctg ccgacgtgcc gcgcgagctg cacgtgctgt tcatcggggc cgacgccgca
    22441 cgctcggcgc gcgcgcttgt cgaggcggcg cgcgcgcgcc acgcgctcat cgtcacggac
    22501 ctgcccgacg ggctcgccga cggcgccgcg gtgcagttcg tgcaggtcga cggccggctg
    22561 cgcttcgaga tctcgctggc cggggcgcgc cggtgcgggc tcaagctcag ctcgcagctg
    22621 atgggcgtgg cctggaaagt gatggaggac gggcaatgac gactcccgcg ctgcgcgcac
    22681 ggctgacccg cgccttcctg ctgacgatcg ccgcggcgtt gctgctggac gccgtcctcg
    22741 tgctcggctt cgagttccag cgtttccgca gcgaggcgat cgacaacctg cacacgcagc
    22801 agagcatcct ggcgcgcgcg gtgacgccgt cgctcgtgtt caacgacccg gacgccgcgg
    22861 cgcagcagct ggcctcgctg gccgagaacc cgcgcgttcg catggccgag gtcttcggtg
    22921 cgaacggccg gccgttcgcg cgctacacgg ccagcaagga cgatgcggcg cgcctgccgg
    22981 ccagcaaccc gctgccgggc ctgggcagca acttccaggg cgacgacgtg gagatcgcct
    23041 acgacatctt ccatgacggc gagcgcatcg gcacgctgta cctgcgcgcg cgcagcgacg
    23101 tcgcgcgccg gctggccggc tacgccgtca tccaggcact ggtgatggcg gtctcgctgg
    23161 gcctggcgct gtcggtgttc gggcgcgtgc agcgccgcat cacgcagccg atctcgcgcg
    23221 tggcccgcgt ggcgcaggag gtggtcgagc ggcgcgactg gcggctgcgc gcgccgtcgg
    23281 ccgacacgcg cgagatcgag gcactggtcg acgcgttcaa ccgcatgctc gccgagatgg
    23341 gcgagagctc gacgcagctg cgccaggaga tcggcgagcg cgaacgggcc gaggacggcc
    23401 tgcgcgcggc cgatcgccgc aaggacgaat tcctcgcgac gctcgcgcac gagctgcgca
    23461 acccgctggc gccgatgctc aacgccgtcg cgctgatgcg ccgcggcgac ctcgcgccgg
    23521 cggtgcgcga caaggcgttg agcatcctcg agcgccagct gcgccacgtg gtgcggctca
    23581 tcgacgacct gctggacgtc tcgcgcatca ccacgggcaa gctgtcgctg cacatggaga
    23641 tcgtcgacct gcacgcggtc atccgcccga cgatggagct ggccgagacg accgcgcacg
    23701 accgccagct gcagttgacg tccgcggtgc ccgatgcgcc gtgctgggtg gtgggcgaca
    23761 ccgcgcggct gcagcaggtg ttctccaacc tgcttggcaa cgcctgccgc tacacgccgc
    23821 cgggcggccg catcgaggtg acggcgcgct gcgaggacgg gttcgccatc gtcgaggtcg
    23881 ccgacaccgg cgtgggcatc gacccgcaga tgcatgagcg cgtgttcgag ctgttcgagc
    23941 agggcgacaa gagcctggag cgcggcaacg cgggcctggg catcgggctc acgctcgcgc
    24001 gccagctggt gctgctgcac ggcggcacga tcggcgtgtc cagccgcggc atcggccacg
    24061 gctcgacgtt ccgcgtccgc c
//
LOCUS       DATCRO010000461         4143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_443644, whole genome shotgun sequence.
ACCESSION   DATCRO010000461 DATCRO010000000
VERSION     DATCRO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443644"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1022)
                     /gene="rapZ"
                     /locus_tag="VIN75_12610"
     CDS             complement(87..1022)
                     /gene="rapZ"
                     /locus_tag="VIN75_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8975056.1"
                     /translation="MSSGSDQHVPGTQSEEPAAGLDGTREIILITGISGSGKSVALHA
                     LEDAGFFCVDNLPPELLPEFLRLESERAVRRVAVAVDVRTAGSLPRLVPLLEKLMNEG
                     VKVRSVFLDSSTDTLVRRFSETRRPHPLSKGQFGTDTHRALIEAIELERQLLATIRVR
                     STILDTSQLRPIQLRQWIRDLVGAKSHNLSLVFESFAFKRGVPLDADMVYDVRVLPNP
                     YYLRELRPLNGRDVEVATYLREQPEVMEMLDQIEAFLRRWLPNFADDQRSYLTVAIGC
                     TGGQHRSVFMAETLAQRFSGSFITLLRHRELDAAE"
     gene            complement(1060..2796)
                     /gene="recN"
                     /locus_tag="VIN75_12615"
     CDS             complement(1060..2796)
                     /gene="recN"
                     /locus_tag="VIN75_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8975057.1"
                     /translation="MLRRLSLKDFVIVDALELEFGAGFSVLTGETGAGKSILIDALQL
                     ALGARAEGGVVREGCARSEIAAEFDSPPSLAGWLDEAGFAVDETLLLRRVVDAQGKSR
                     AFVNGSAATLVQLREVADHLIDIHGQHAWQSLTRPAAVRALLDEQAGIEAAPVAAAHA
                     AWAQATAALAGARERQGELDNERERLAWQLQEVERLAPSDDEWQAITAEHGRLSHAQA
                     LMSGSQQALEDVSEGELNALKLVSRAASALREVADYDAALAPVIEALDGAELQLQDAA
                     HSLSSYLNRADLDPQRLAELDDRMSSWMSLARRFRRSPEDLPALWGEWKTALAALDAA
                     ADLAGLQKKVDDAVARWRTEAKRVSARRAQAAPQMAARITEAMQQLGMAGGRFDVALV
                     AQESPQSYGAETVEFLVAGHAGSTPRPLAKVASGGELSRLALAIAVTAAQSSAGGDGA
                     AAPRVASPAVGTLIFDEIDSGVGGTVADSVGRLMKRLGRHVQVMAVTHLPQVAACGDH
                     HFVVAKRSVGGNTTSQVTPLSGEARVAEVARMLGGERSSGTSLAHAQELIAQAVAVDG
                     AAPPPRGRSGAR"
     gene            complement(2956..3828)
                     /locus_tag="VIN75_12620"
     CDS             complement(2956..3828)
                     /locus_tag="VIN75_12620"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY8975058.1"
                     /translation="MSTPFRHVALFGKYQAQGVRPILEEIAQFLVRVGVEVSVDHDTA
                     MNTDWLDFGAMRIDELAQVCDLAVVVGGDGTMLGIGRELARHGIPLVGINQGRLGFIT
                     DIPATSWRESLAPIVKGDYEAESRPMIEGGVWRDGEQIFQGFALNDVVVRSSTSSMIE
                     VRVSVGADHVANIRSDGVIVASPTGSTAYALSAGGPILHPGIGGWTMVPVASHTLSNR
                     PIVLPDDQEVLLELVQGKEASANFDMHGLASMLLGDIVRMQRSAHKARFLHPRGWSYY
                     ATLRHKLHWHEGTP"
     gene            4005..>4143
                     /locus_tag="VIN75_12625"
     CDS             4005..>4143
                     /locus_tag="VIN75_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8975059.1"
                     /translation="MLDERAKTLLKTLVERYIADGQPVGSRTLSRASGLELSPATIRN
                     VM"
BASE COUNT          588 a         1459 c         1518 g          578 t
ORIGIN      
        1 cgcgacggcc tcgcgatcgg cggccggcag ggtgcgccag tccgccagtt cgacgcgcgt
       61 tgcggcgccg cgcccgggca gggcgctcat tccgctgcgt cgagctcgcg gtggcgcagc
      121 aacgtgatga agctgcccga gaaccgctgg gcgagcgtct cggccatgaa gaccgagcgg
      181 tgctgcccgc cggtgcagcc gatcgcgacg gtgaggtagc tgcgctggtc gtcggcgaag
      241 ttgggcagcc agcggcgcag gaaggcctcg atctggtcga gcatctccat gacctccggc
      301 tgctcgcgca ggtaggtggc cacctcgacg tcgcggccgt tgagcgggcg cagctcacgc
      361 aggtagtacg ggttgggcag cacgcgcacg tcgtagacca tgtcggcgtc gagcggcacg
      421 ccgcgcttga acgcgaacga ttcgaagacg agcgacaggt tgtgcgactt cgcgccgacc
      481 aggtcgcgga tccactggcg cagctggatg gggcgcagct ggctcgtgtc caggatggtg
      541 gagcgcacgc ggatggtggc gagcaactgg cgctcgagct cgatggcctc gatcagggcg
      601 cggtgggtgt cggtgccgaa ctggcccttg gacagcgggt gcgggcggcg cgtctccgag
      661 aagcggcgca ccagcgtgtc ggtgctggaa tcgaggaaca ccgagcgcac cttgacgccc
      721 tcgttcatca gcttctcgag cagcggaacg aggcgcggca gcgagccggc ggtgcgcacg
      781 tcgacggcca ccgccacgcg gcgcacggcg cgctcgctct cgagccgcag gaattcgggc
      841 agcagctcgg gcggcaggtt gtcgacgcag aagaagccgg cgtcctccag cgcatgcagc
      901 gcgacggact tgcccgagcc cgagatgccg gtgatcagga tgatctcgcg cgtgccgtcg
      961 aggccggcgg cgggctcctc ggactgcgtg ccggggacgt gttggtcgga gccggagctc
     1021 atggccattc cttgatgatg atgggacggg gcgcgggcat cagcgcgccc cggagcgacc
     1081 gcgcggcggc ggcgcggcgc cgtccacggc caccgcctgt gcgatcagtt cctgcgcgtg
     1141 ggccaggctg gtgccggagc tgcgttcgcc gcccagcatg cgcgcgacct cggccacgcg
     1201 cgcctcgccc gacagcggcg tgacctggct ggtggtgttg ccgccgacgc tgcgcttggc
     1261 gaccacgaaa tgatggtcgc cgcaggccgc gacctgcggc aggtgcgtga cggccatgac
     1321 ctggacatgg cggcccagcc gcttcatgag acgaccgacc gagtcggcca ccgtgccgcc
     1381 gacgcccgag tcgatctcgt cgaagatcag cgtgcccacc gcaggcgacg cgacgcgcgg
     1441 cgccgccgcg ccgtcgccac cggccgacga ctgggcggcc gtcaccgcga tggccagcgc
     1501 caggcgcgac agctcgccgc ccgaggcgac cttggccagc ggccgcggcg tgctgcctgc
     1561 gtggccggcg acgaggaact ccaccgtctc cgcaccgtag gactgcggcg actcctgcgc
     1621 gacgagcgcg acgtcgaagc gcccgccggc catgcccagc tgctgcatcg cctcggtgat
     1681 gcgcgcggcc atctgcggcg cggcctgcgc gcggcgggcg ctcacgcgct tggcctcggt
     1741 gcgccagcgc gcgaccgcgt cgtcgacctt cttctgcagc ccggccaggt cggcggcggc
     1801 gtcgagcgcg gccagcgccg tcttccactc gccccacagc gcgggcaagt cttccggcga
     1861 gcggcggaag cggcgcgcca ggctcatcca cgacgacatg cggtcgtcga gctcggccag
     1921 gcgctgcggg tcgaggtcgg cgcgattcag gtagctggag aggctgtgcg cggcgtcctg
     1981 cagctgcagc tccgcgccgt cgagcgcctc gatgacgggc gccagcgcgg cgtcgtagtc
     2041 ggcgacctcg cgcagcgcgc tggccgcgcg cgacaccagc ttgagcgcat tgagctcgcc
     2101 ctcggacacg tcctccagcg cctgctgcga gcccgacatc agcgcctgcg catgcgacag
     2161 ccggccgtgc tcggcggtga tggcctgcca ctcgtcgtcg gatggcgcca ggcgctcgac
     2221 ctcctgcaac tgccacgcca ggcgctcgcg ctcgttgtcg agctcgccct ggcgctcgcg
     2281 cgcgcctgcg agcgccgcgg tggcctgcgc ccaggccgca tgcgcggcgg ccaccggggc
     2341 ggcctcgatg ccggcctgct cgtcgagcag cgcgcgcacc gcggcagggc gggtgaggct
     2401 ctgccaggcg tgctggccgt ggatgtcgat caggtgatcg gccacctcgc gcaactgcac
     2461 gagcgtggcg gcgctgccgt tgacgaacgc gcggctcttg ccctgcgcat cgaccacgcg
     2521 gcgcagcagc agcgtctcgt cgacggcgaa gccggcctcg tcgagccagc cggccagcga
     2581 cggcggggag tcgaactcgg ccgcgatctc gctgcgcgcg cagccctcgc gcaccacgcc
     2641 gccctcggcg cgcgcgccga gcgccagctg cagcgcgtcg atcaggatgg acttgccggc
     2701 gcccgtctcg ccggtgagca ccgagaagcc ggcgccgaac tccagttcga gcgcatcgac
     2761 gatgacgaaa tctttcagcg acaggcggcg cagcatgatg cgggcaggga aggtgcgttg
     2821 cgttggaacg aaggcgccgg gcagggggct cgacgatgcg agccgcggat ggtgaccggc
     2881 cgcgggctca tgccgtgagc ctgtgcgacg gcggatgcgg cggcaggcat cggtcggcgc
     2941 gcggcggcgg acatgtcagg gtgttccttc gtgccagtgc agcttgtgcc gcagcgtggc
     3001 gtagtagctc cagccgcgcg ggtgcaggaa ccgcgccttg tgggccgagc gctgcatgcg
     3061 cacgatgtcg cccagcagca tgctggccag gccatgcatg tcgaaattgg cgctggcctc
     3121 cttgccctgc accagctcga gcagcacctc ctggtcgtcg ggcaggacga tggggcggtt
     3181 ggacagcgtg tgcgacgcca ccggcaccat cgtccagccg ccgatgcccg gatgcaggat
     3241 cggcccgccg gccgacagcg cgtaggccgt ggagcccgtg ggcgaggcga cgatgacgcc
     3301 gtcggaacgg atgttggcca cgtggtcggc gcccaccgac acccgcacct cgatcatgct
     3361 ggacgtgctg ctgcgcacca cgacgtcgtt gagcgcgaag ccctggaaga tctgctcgcc
     3421 gtcgcgccag acgccgcctt cgatcatcgg ccggctctcg gcctcgtagt cgcccttgac
     3481 gatgggcgcg agcgactcgc gccagctggt ggccggaatg tcggtgatga agcccagccg
     3541 cccctggttg atgcccacca acgggatgcc gtggcgcgcg agctcgcggc cgatccccag
     3601 catggtgccg tcgcccccga ccaccacggc gaggtcgcac acctgggcga gctcgtcgat
     3661 gcgcatggcg ccgaagtcga gccagtcggt gttcatcgcg gtgtcatgat cgaccgagac
     3721 ttccacgccc acccgcacca ggaactgcgc gatctcctcc aggatgggac gcacgccctg
     3781 ggcctggtac ttgccgaaca gcgcgacgtg gcggaagggg gtggacatgc gatgaattac
     3841 accacagggc cctcgtggcg aggggtgcat tccatgccgt gaaaccgcct gtttcgcgct
     3901 gggaacgggg tcgcgacggg tcccttgcgc attggccagc ctcgcgtgaa atcccgaatc
     3961 ctgccgcccc ggcaagcgcc accccatctc cgtacaatcg acccatgctc gacgagcgcg
     4021 ccaagacact gctgaagacc ctggtcgaac gctacatcgc ggacggccag ccggtgggtt
     4081 cgcgcaccct gtcgcgagcc tccggcctgg aactgtcgcc ggccaccatc cgcaacgtga
     4141 tgg
//
LOCUS       DATCRO010000462        10396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_949689, whole genome shotgun sequence.
ACCESSION   DATCRO010000462 DATCRO010000000
VERSION     DATCRO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949689"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..584)
                     /locus_tag="VIN75_12630"
     CDS             complement(330..584)
                     /locus_tag="VIN75_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY8975060.1"
                     /translation="MKQSIIAVSLALVASASFARAAHAPDPAASGAVATAAATEPGTP
                     QRSKMKTCNADARSKDLHGPDRKAFMKQCLSGKGKPAAAS"
     gene            complement(847..1152)
                     /locus_tag="VIN75_12635"
     CDS             complement(847..1152)
                     /locus_tag="VIN75_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY8975061.1"
                     /translation="MKQLITAIALSVAAAGVFAADAAASKPLTAQQQKMADCSAANKG
                     KKGDEYKAAQKACLSGQAAPASAPMTQKEKMAYCSKQNKGKKGDDYKAAQKACLSAS"
     gene            complement(1500..2867)
                     /locus_tag="VIN75_12640"
     CDS             complement(1500..2867)
                     /locus_tag="VIN75_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8975062.1"
                     /translation="MSDSAFNAELTERDLPRGELPSSIGKYTVLGRLGDGATSEVFRA
                     RDDEKDREVAIKRLRLSSDTAAVDAHFWSRFFAAEAALVGRLNHPNVVQLYEAFSDAQ
                     VPYLVMEYVPGVTLRDFCRPDRLLPLEQVVEIGFKCAMALGYVFRQGLIHRDVKPANI
                     LTVMQDGEVVDVKLSDFGSVLNTNSEATQIHRVGSLAYMSPEQIEGNALDCRTDIYAL
                     AAVLYHLVSGRPPFDAQTQVALMNQIFHNQPPPLTSLREGVTPALENVILSALAKNRE
                     DRPQDWESFAQALSGLIVNHQVPRGRLQKVLDSERFNLLRSLDFFSAFDDVELWEVVH
                     RAKWLRLHYGHRVYRRGQEGNTFHIIAQGEVEVFRDGQRVARLGAGTSVGEMAYLAPS
                     PDLRRHSADIIVSEPCTTVSFTPESLTQLSAETRMRFDKAFIQVLVRRLHAAHETLAH
                     PRRVL"
     gene            3051..4787
                     /gene="argS"
                     /locus_tag="VIN75_12645"
     CDS             3051..4787
                     /gene="argS"
                     /locus_tag="VIN75_12645"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8975063.1"
                     /translation="MIQAKSELAQALAAAVAQVAPDVAFTPAFESPKQAAHGDLAITV
                     AMQLAKPLRLAPRAVAEKLVAALNEQPAVQRWVSALEIAGPGFINLRLSPAAKQAVVT
                     EVLAAGERFGIQPSNGNKVIVEFVSANPTGPLHVGHASQAALGSTVCNLLASQGWAVQ
                     REFYYNDAGVQIGTLAHSTQLRIKGFKPGDAVWPTDPDNPESKKFYNGEYIADIAADF
                     LAKKTVKADDREFTASGDPDDLDSIRQFAVAYLRHEQDLDLQAFGVHFDNYFLETSLY
                     KNNAVETTVAKLIASGKTYEADNALWLRTTEYGDDKDRVMRKSDGTYTYFVPDVAYHL
                     DKWERGFAKAINVQGSDHHGTVARVRAGLQAAGEGVPQGWPDYLLHKMITVMRGGEEV
                     KISKRAGSYVTLRDLIDWTSRDAVRFFLVSRKADTEFVFDIDVALKQSDENPVFYVQY
                     AHARICSVLRQFADKGGDLSRVDGADLSLLTAPSEAALMLQLAAFPETLTAAARDLAP
                     HDIAYYARGVAASFHSYYAAERFLVDDPALTLARLALLKASGQVLRNALAILGVSAPE
                     QMNRDEPAASAS"
     gene            4816..5487
                     /locus_tag="VIN75_12650"
     CDS             4816..5487
                     /locus_tag="VIN75_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8975064.1"
                     /translation="MKNQRGGFALGFVVGLLAGLAVALAVALYVTNAKVPLLSKGQQR
                     TPEQDAAEAERNKNWDPNAALAGKPPVRAVAPAAPSAPASDTAIVPHTTGPVLPPPPE
                     TAKASASAKNVETPKSTRDAAAILNGSDPAESKTASAGADPFIYFVQVGAYSKPEDAE
                     QQRARLALAGLRATVTERDQNGRTVHRVRLGPFDKRDEAEAQTDRAKAIDPDASLVRV
                     DRSKQ"
     gene            5544..6197
                     /locus_tag="VIN75_12655"
     CDS             5544..6197
                     /locus_tag="VIN75_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8975065.1"
                     /translation="MNRREFSVQLAGVAGAAALSALLPGLAFASPAAPVEGTDYDRLK
                     TPVSLPKTGKVEVLEFFWYGCPHCNHFEPTIEPWIARLPADVHFRRVHVQFNARQAIH
                     QRIYYTWEMMGLVDKMHARTFERFHVQRKPIDSEDDMLAFARENGLDVAKVKSTWEGF
                     GMGTRVAQAQQLCDDYRVDGVPMIGIHGRYTTSASQGGEAACLATTDSLIAQLRKTA"
     gene            6202..6861
                     /locus_tag="VIN75_12660"
     CDS             6202..6861
                     /locus_tag="VIN75_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8975066.1"
                     /translation="MDRRRFSLNAASLAGALALAGAGLPGRARAQQGTPVEERDYQTL
                     VRPVAVPPDGRIEVIEFFWYGCPHCFAFEPVIEPWIAKLPADVHFRRVPVGFDTRKQV
                     HQRIFYTWEALGLVEQMHARTFARFHVQKKPIDSLDDMLVFAKENGLDTAKVESAWRG
                     FGTDVKCKEANQLEDAYGIEYMPEMAVAGRFIAVAQPALGHASVLVTTDWLIDRVRQH
                     R"
     gene            6974..7654
                     /gene="lptA"
                     /locus_tag="VIN75_12665"
     CDS             6974..7654
                     /gene="lptA"
                     /locus_tag="VIN75_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY8975067.1"
                     /translation="MPITMRPLTTPFPTEAARRPGVAGSRTALALAAAALATLCAGAH
                     AEKADRGKPMSVTSAGQAPDVVDLKKHSAVFTGDVVITQGTLEIHADRVEVSEDPDGQ
                     RLGYAFGSASKPATFRQKRDGVDEYSEGDAQRIEYDSAANRVRFVGAAHLRMLKGTVV
                     TDQASAALITYDTATDTVKLGSGGSEGAAASSAGGRTTVVFTPKAPSSAPPPKPLELP
                     TPASGSGK"
     gene            7651..8520
                     /gene="lptB"
                     /locus_tag="VIN75_12670"
     CDS             7651..8520
                     /gene="lptB"
                     /locus_tag="VIN75_12670"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY8975068.1"
                     /translation="MTDESPSTAQVKPGTAVSPSATPVTPPPVGSQPVHAPAAGAASQ
                     SRLEAAGLRKSYGARLVVKEVHLAVNAGEVVGLLGPNGAGKTTTFYMVVGLVRADAGD
                     IRIDGQPISAKPIHQRSRLGLSYLPQEASIFRKLTVDENIRAVLELQYDENGKALKPA
                     DIDKRLDGLLNDLSLTHLRDSPAPALSGGERRRVEIARALATQPRFILLDEPFAGVDP
                     IAVIEIQRIIGFLKARGIGVLITDHNVRETLGICDRAYIISEGRVLAEGTPAEIVENP
                     DVRRVYLGEHFRM"
     gene            8544..10103
                     /locus_tag="VIN75_12675"
     CDS             8544..10103
                     /locus_tag="VIN75_12675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8975069.1"
                     /translation="MKASLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEIEQMMEQN
                     PFLEPEEDASAPELPFDAPDASASAGSGDTVKAASDAEADSRAGGEGGDEPAGVDAAD
                     FGTTERDDWENGTERDDFDGIRELPSSTSGQSGSDDDDFERQNRANAGVTLEDHLRTQ
                     LSSLRLSPEDAAAVHVLIESLNEDGYLADTLEEIAERLAEVLGVQGEEEREELMDRLQ
                     CGLNFLQSMEPTGVGARSLGECLCLQLRQKTPAKATAVTLLAALKICTEHLALLAKRD
                     FKKIAQLIEVDEPTVRAAQGLIVGLEPKPGRPFTRAEEQVIVPDVIVMKVARQWKAVL
                     NPDVMPKLRVNDLYAQAIKSQKGSGSQLSARLQEARWFIRNIQQRFDTILRVSQAIIE
                     RQKNFFSHGAIAMKPLVLREIADELGLHESTISRVTTAKYMATPFGTYELKYFFGSSL
                     NTDAGGNASSTAVRALIKQLVAAESPAKPLSDSQLAEMLEGQGIQVARRTVAKYREAL
                     KIAPANLRRTV"
     gene            10165..>10396
                     /locus_tag="VIN75_12680"
     CDS             10165..>10396
                     /locus_tag="VIN75_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY8975070.1"
                     /translation="MTTDFLFVTCANGSEQLLAEECARILGTTQGIEAGRGGVMVDEN
                     VEHAMRLNLHSRLAQRVLWPMVDGEYRDERDLY"
BASE COUNT         1689 a         3709 c         3541 g         1457 t
ORIGIN      
        1 tggcgggtta cgcctgcgcc aacccgccct gcggatacga cccaaagcaa atggcgacgt
       61 cccgggaggg acgccgcagg gcgggtcagc cgcaggcgta acccgccatc gcgtcaacgc
      121 gcggcctgtc gttcgtccgg cagatcggtt ccaccgaggc ccaccgaccg gcgcggcggt
      181 ggccagggtg ccgccacacg ccggcgggcg cggtgccgcg cccgcgcggc gtcctccgaa
      241 cgaggcctcg actcgggcgc cgcagcgatc ccccgacccg tgctctgcat cggaaacgcg
      301 aaacgccccc gcatggcggc ctcgcgtgct caggaggccg cggccggctt gcccttgccc
      361 gacaggcact gcttcatgaa cgccttgcgg tcggggccgt gcaggtcctt ggacctggcg
      421 tcggcgttgc acgtcttcat cttcgagcgc tgcggcgtgc ccggctcggt ggcggccgcg
      481 gtggccacgg cgccggaggc ggccgggtcg ggcgcgtgcg cggcgcgcgc gaaggaggca
      541 ctcgcgacga gggcgagcga gacggcgatg atcgattgct tcatgggttt cctcgaaagt
      601 cgttgaatgg actgctcgcg tgcagcccgg cacgaggccg ggcgacacgc gccagcccat
      661 gctgcgttcg atcgaggaaa cgccccggcg ccgaatggcc gatttgcggc agccgcgcgg
      721 caatcgccat cgaaatgggg tgaaccgcgc acgacggcgc aggcgccgcc cggaaacgac
      781 gaacccggcg ctcggccggg ttcgttggcg ggcccgcgcg aggcgggcgc gggcgcgcgg
      841 cgccgctcag gaggccgaca ggcaggcctt ttgcgcggcc ttgtagtcgt cacccttctt
      901 gcccttgttc tgcttggagc agtaggccat cttctccttc tgcgtcatcg gggccgaggc
      961 gggcgcggcc tggcccgaca ggcaggcctt ctgcgcggcc ttgtactcgt cgcccttctt
     1021 gcccttgttg gcggcggagc agtcggccat tttctgctgt tgcgccgtca gcggcttgct
     1081 ggcggccgcg tcggcggcga agacgccggc agcggcgaca gacagggcga tcgcggtgat
     1141 cagttgcttc atggtgtgtg tcctctctag cgcctctggt cggcggtggg ttgcaccgtc
     1201 ggcgacggca cggacgcatt gaagcaaacg cgatcggcgg cgacaagcag aaattgcccc
     1261 gtcaaccaca aggttcacgg cgcgttgcat tcgctgtggc ggcgagccga cgccagcgtg
     1321 acccttcgca aggtcgtcac cgaaagatgt ctctcgagcg aacttgttcg ccggcgcact
     1381 ccccggcgct gccgctcgct cgatccgcgc cagattcaag ggagcggcgg aaccggctcc
     1441 gccgggccgc tcgcggagcg ccccctcggg gggcagcgag cgccagcgag cgtgggggct
     1501 cacagtaccc ggcgcggatg ggccagcgtc tcgtgcgcgg cgtgcagccg ccgcaccagc
     1561 acctggatga acgccttgtc gaagcgcatg cgcgtctcgg cgctgagctg cgtcaacgac
     1621 tcgggcgtga acgagaccgt ggtgcacggc tcggagacga tgatgtcggc gctgtggcgg
     1681 cgcaggtcgg ggctgggtgc gaggtaggcc atctcgccca ccgacgtgcc cgcgcccagg
     1741 cgcgccacgc gctggccgtc gcggaagacc tccacctcgc cctgcgcgat gatgtggaag
     1801 gtgttgccct cctgcccgcg ccggtacacg cggtggccgt agtgcagccg cagccacttg
     1861 gcgcggtgca ccacttccca cagctcgacg tcgtcgaacg cggagaagaa gtccagcgag
     1921 cgcagcaggt tgaagcgctc ggagtcgagc accttctgca agcgtccgcg cggcacctgg
     1981 tggttgacga tcagccccga caacgcctgc gcgaagcttt cccagtcctg cggccggtcc
     2041 tcgcggttct tggcgagcgc gctcaggatc acgttctcca gcgcgggcgt gacgccctcg
     2101 cgcaggctgg tgagcggcgg cggctggttg tggaagatct ggttcatcag cgcgacctgg
     2161 gtctgcgcgt cgaacggcgg ccggccggag accaggtggt acagcacggc ggccagcgcg
     2221 tagatgtcgg tgcggcaatc gagcgcgttg ccctcgatct gctcgggcga catgtaggcc
     2281 agcgagccca cgcggtggat ctgcgtcgcc tcggaattgg tgttgagcac gctgccgaag
     2341 tcgctcagct tgacgtcgac gacctcgccg tcctgcatca cggtcaggat gttggccggc
     2401 ttgacgtcgc ggtggatcag cccctggcgg aagacgtagc ccagcgccat cgcgcacttg
     2461 aagccgatct cgaccacctg ctccagcggc agcaggcggt ccgggcggca gaagtcgcgc
     2521 agcgtcacgc cgggcacgta ctccatgacg aggtagggca cctgggcgtc gctgaacgcc
     2581 tcgtacagct gcaccacgtt ggggtggttc aggcgcccga ccagcgccgc ctcggcggcg
     2641 aagaagcgcg accagaagtg cgcgtcgaca gcagccgtgt cggacgacag gcgcagccgc
     2701 ttgatcgcga cctcgcggtc cttctcgtcg tcgcgcgccc ggaacacctc gctcgtggcg
     2761 ccgtcgccca ggcggccgag caccgtgtac ttgccgatcg acgacggcag ttcgccgcgc
     2821 ggcaagtcgc gctcggtcag ctcggcgttg aaggcggaat cgctcatcgg gacggggttt
     2881 gttgtcgggg acaccggtgc ctggcttcca ggccgccatg cgtcaggttc gatattgatg
     2941 cctcccgggt gtaaccgatc cttcgagaag gggtcgattc ccgggttgtt cggtcgccct
     3001 ggcccgactg tcggacggat cacgagggcc gcgaaccgta gaatccctgc atgatccaag
     3061 ccaaatccga gctggcgcaa gccctggcgg ccgcggtcgc ccaggtggcg ccggacgtcg
     3121 cgttcactcc cgcgttcgag tcccccaagc aggccgcgca cggcgacctg gccatcaccg
     3181 tcgccatgca gctggccaag ccgctgcgcc tggctccgcg cgccgtcgcc gaaaagctgg
     3241 tcgccgcgct gaacgaacag ccggccgtgc agcgctgggt gtccgcgctc gagatcgcgg
     3301 gcccgggctt catcaacctg cgcctgtcgc cggccgccaa gcaggcggtc gtcaccgagg
     3361 tgctggccgc cggcgagcgc ttcggcatcc agccgtccaa cggcaacaag gtgatcgtcg
     3421 agttcgtgtc ggccaatccg accgggccgc tgcacgtggg ccacgccagc caggcggcgc
     3481 tcggatcgac cgtgtgcaac ctgctcgcca gccagggttg ggcggtgcag cgcgagttct
     3541 actacaacga cgcgggcgtc cagatcggca cgctggccca cagcacgcag ctacgcatca
     3601 agggcttcaa gcccggcgac gccgtctggc cgacggatcc ggacaatccg gagagcaaga
     3661 agttctacaa cggcgagtac atcgccgaca tcgccgccga cttcctcgca aagaagacgg
     3721 tcaaggccga cgaccgcgag ttcaccgcct cgggcgaccc ggacgacctc gactcgatcc
     3781 gccagttcgc cgtcgcctac ctgcgccacg agcaggatct cgacctgcag gccttcggcg
     3841 tgcacttcga caactacttc ctcgagacca gcctctacaa gaacaacgcg gtcgagacga
     3901 cggtggccaa gctcatcgcc tcgggcaaga cctacgaggc cgacaacgcg ctgtggctgc
     3961 gcaccaccga gtacggcgac gacaaggacc gcgtgatgcg caagtccgac ggcacgtaca
     4021 cgtacttcgt gcctgacgtc gcctaccacc tggacaagtg ggagcgcggc ttcgccaagg
     4081 cgatcaacgt gcagggcagc gaccaccacg gcaccgtcgc ccgggtgcgc gcgggcctgc
     4141 aggccgcggg cgaaggcgtg ccccagggct ggcccgacta cctgctgcac aagatgatca
     4201 ccgtcatgcg cggcggcgag gaggtcaaga tctccaagcg cgccggcagc tacgtgacgc
     4261 tgcgcgacct gatcgactgg accagccgcg acgccgtgcg tttcttcctc gtcagccgca
     4321 aggccgatac cgagttcgtg ttcgacatcg acgtcgcgct caagcagagc gacgagaacc
     4381 cggtgttcta cgtccagtac gcgcatgcgc gcatctgctc ggtgctgcgc cagttcgccg
     4441 acaagggggg cgacctgtcc agggtggacg gcgccgacct gtcgctgctg acggcgccgt
     4501 ccgaggctgc gctgatgctg cagctcgctg cgttccccga gacgctgacg gccgccgcgc
     4561 gcgacctcgc gccgcacgac atcgcgtact acgcccgtgg cgtcgccgcc agcttccact
     4621 cgtactacgc cgccgagcgg ttcctcgtcg acgaccccgc gctgacgctt gcgcggctgg
     4681 cgctgctcaa ggcctcgggc caggtgctgc gcaatgcgct ggccatcctg ggcgtcagtg
     4741 cgcccgagca gatgaaccgc gacgaacccg ctgcttccgc ctcctgatca ttcggaaccc
     4801 ctcgtgaaac cttccatgaa gaaccaacgg ggcggcttcg ccctgggctt tgtcgtcggc
     4861 ctgctggcgg gcctggccgt cgcgctcgcg gtggccctgt acgtcaccaa cgccaaggtg
     4921 ccgctgctga gcaagggcca gcaacgcacg cccgagcagg acgccgccga ggccgagcgc
     4981 aacaagaact gggacccgaa cgccgcgctg gcgggcaagc cgcccgtgcg ggcggtcgcg
     5041 cccgcggccc cgtcggcgcc ggcctccgac accgccatcg tgccgcacac gaccggcccg
     5101 gtgctgccgc cgccgccgga gacggccaag gcgtcggcct ccgcgaaaaa tgtggaaacc
     5161 cccaagagca cccgcgacgc cgccgcgatc ctgaacggca gcgacccggc cgaatcgaag
     5221 actgcatccg ccggcgccga tcccttcatc tatttcgtgc aagtgggcgc atactccaaa
     5281 cccgaagacg ccgagcagca gcgcgccagg ctggcgctcg caggcctgcg ggcgacggtg
     5341 accgagcgcg accagaacgg ccggactgtc caccgagttc gcctcgggcc gtttgacaag
     5401 agggacgagg ccgaggcgca gaccgaccgc gccaaggcga tcgaccccga cgcgtcgctg
     5461 gtgcgcgtcg accgctcgaa acagtgaact tcgtggccgg atcgtcgctc catcggcgtc
     5521 cggcgccccc tgaaaggatc ttcatgaacc ggcgcgagtt ttccgtgcaa ctggccggcg
     5581 tggccggcgc cgcggcgttg tccgccctgc ttcccggcct ggcgttcgcg tcgcccgccg
     5641 ccccggtcga aggcaccgac tacgaccggc tgaagacgcc ggtcagcctc cccaagaccg
     5701 gcaaggtcga ggtgctggag ttcttctggt acggctgccc gcactgcaac catttcgagc
     5761 cgacgatcga gccgtggatc gccagactgc ccgcggacgt gcacttccgc cgcgtgcacg
     5821 tgcagttcaa tgcgcgccag gccatccacc agcgcatcta ctacacgtgg gagatgatgg
     5881 gcctggtcga caagatgcac gccaggacgt tcgaacgatt ccacgtgcag cgcaagccga
     5941 tcgacagcga ggacgacatg ctggcgttcg ccagggagaa cggtctcgat gtcgccaagg
     6001 tcaagagcac ctgggaaggc ttcggcatgg gcacccgcgt cgcgcaggcg cagcagctgt
     6061 gcgacgacta ccgcgtggac ggcgtgccga tgatcggcat ccacggccgc tacaccacct
     6121 cggcgtcgca ggggggcgag gccgcgtgcc tggccaccac cgattcgctg atcgcgcagc
     6181 tgcgcaagac agcctgacgc gatggatcgc cgccgcttct cgctgaacgc cgccagcctg
     6241 gcgggcgcgc tggcgctcgc gggcgccggc ctgcccgggc gcgcccgcgc gcagcaagga
     6301 acgccggtcg aggaacgcga ctaccagacg ctcgtgcgcc cggtcgccgt gccgcccgac
     6361 ggcaggatcg aggtcatcga gttcttctgg tacggctgcc cgcactgctt cgcgttcgag
     6421 cccgtcatcg agccgtggat cgcgaagctg ccggccgacg tgcacttccg ccgcgtgccg
     6481 gtgggcttcg acacgcgcaa gcaggtccac cagcgcatct tctacacatg ggaggcgctc
     6541 gggctcgtcg aacagatgca cgccaggacc ttcgcgcgct tccacgtgca gaagaagccc
     6601 atcgactcgc tcgacgacat gctggtcttc gccaaggaga acgggctgga cacggccaag
     6661 gtcgagagcg cgtggcgcgg cttcggcacg gacgtcaagt gcaaggaagc caaccagctg
     6721 gaagacgcct acggcatcga gtacatgccg gagatggcgg tggccggccg cttcatcgcg
     6781 gtggcccagc cggccctcgg acatgccagc gtgctggtca cgaccgactg gctgatcgac
     6841 cgcgtgcgcc aacatcgctg acccgcgccg ccgcagcgtt gggcggggcc gacacctcga
     6901 acaacccggc tcaggaaggg gcgtcgaagt gcccaccggc gaggcgcgcg ggctagaatg
     6961 gtctgcaatt tccgtgccga tcacgatgcg tccgcttaca actcctttcc cgaccgaagc
     7021 cgcccgccgt ccgggcgtcg ccgggtcgcg caccgcgctc gcgctggcgg ctgccgccct
     7081 cgccacgctg tgcgccggcg cgcacgccga gaaggccgac cgcggcaagc cgatgagcgt
     7141 caccagcgcc ggacaggcgc ccgacgtggt cgacctgaag aagcacagcg ccgtcttcac
     7201 cggcgacgtc gtcatcacgc agggcacgct ggaaatccat gccgaccgcg tcgaggtcag
     7261 cgaggatccg gacgggcagc gcctgggcta cgccttcggc tcggcgagca agccggccac
     7321 cttccgccag aagcgcgacg gcgtggatga atacagcgag ggcgacgccc agcgcatcga
     7381 gtacgacagc gccgccaacc gcgtccgctt cgtcggcgcc gcccacctgc gcatgctcaa
     7441 gggcacggtc gtcaccgacc aggcgagcgc cgcgctgatc acgtacgaca ccgccaccga
     7501 cacggtcaag ctgggatccg gcggctccga gggcgcggcc gccagcagcg ccggcgggcg
     7561 caccacggtc gtcttcacgc ccaaggcacc gagctccgcg ccgccgccca agccgctgga
     7621 gctgccgacg ccggcatcgg gatcgggcaa atgaccgacg aatcccccag cacggcgcag
     7681 gtcaagcctg gcaccgcggt ctcgccatcg gccacgccgg tgacgccgcc gcccgtcggc
     7741 tcgcagccgg tgcatgcccc ggccgccggc gccgcgtcgc agagccggct cgaggccgcg
     7801 ggcctgcgca agtcctacgg cgcgcgcctg gtcgtcaagg aagtgcacct ggcggtcaat
     7861 gccggcgagg tggtgggcct gctcgggccc aacggcgccg gcaagacgac gacgttctac
     7921 atggtcgtgg gcctggtgcg cgccgacgcg ggcgacatcc gcatcgacgg ccagcccatc
     7981 tcggccaagc ccatccacca gcgttcacga ctgggcctga gctacctgcc gcaggaagcg
     8041 tccatcttcc gcaagctgac ggtcgacgag aacatccgcg ccgtgctgga gctgcagtac
     8101 gacgagaacg gcaaggcgct caagccggcc gacatcgaca agcgcctgga cggcctgctc
     8161 aacgacctga gcctgacgca cctgcgcgac tcgccggcgc cggcgctgtc cggcggcgaa
     8221 cgccgccgcg tcgagatcgc gcgcgccctg gcgacgcagc cgcgcttcat cctcctggac
     8281 gaaccgttcg ccggcgtcga cccgatcgcc gtcatcgaga tccagcgcat catcggcttc
     8341 ctgaaggcgc gcggcatcgg cgtgctgatc accgaccaca acgtgcgcga gacgctgggc
     8401 atctgcgacc gcgcctacat catcagcgag ggccgcgtgc tggccgaggg caccccggcg
     8461 gagatcgtcg agaaccccga cgtgcggcgc gtgtacctcg gcgaacactt ccgcatgtaa
     8521 ggcgggcttc caggatctcg atcatgaaag cctcgctgca ggtccgcctg tcgcaacacc
     8581 tggcgctgac gccgcagctg cagcagtcca tccggctgct gcagctctcg accctggaac
     8641 tgcaccagga gatcgagcag atgatggagc agaacccctt cctcgagccc gaggaggatg
     8701 cgtccgcgcc cgagctgccg ttcgacgcgc ccgacgccag cgcgtcggcc ggcagcggcg
     8761 acacggtcaa ggccgcgtcc gacgccgagg ccgacagccg cgccggcggc gaaggcggcg
     8821 acgagcccgc cggcgtcgac gccgccgact tcggcaccac cgagcgcgac gactgggaga
     8881 acggcactga gcgcgacgac ttcgacggca tccgcgagtt gccgtcgtcg accagcggcc
     8941 agtccggcag cgacgacgac gacttcgagc gccagaaccg cgccaacgcc ggcgtcacgc
     9001 tcgaggacca cctgcgcacg cagctgagca gcctgcgcct gtcgcccgag gacgccgcgg
     9061 cggtgcacgt gctcatcgag tcgctcaacg aggacggcta cctcgccgac acgctcgagg
     9121 agatcgccga gcgcctcgcc gaggtgctgg gcgtccaggg cgaggaagag cgcgaggagc
     9181 tgatggaccg cctgcagtgc ggcctgaact tcctgcagag catggagccc accggcgtcg
     9241 gcgcgcgttc gctcggcgaa tgcctgtgcc tgcagctgcg ccagaagacg ccggccaagg
     9301 ccaccgcggt cacgctgctg gccgcgctca agatctgcac cgagcacctg gccctgctgg
     9361 ccaagcgcga cttcaagaag atcgcccagc tgatcgaggt ggacgagccc acggtgcgcg
     9421 ccgcgcaggg actgatcgtc ggcctggagc ccaagcccgg tcgccctttc acgcgcgccg
     9481 aggaacaggt gatcgtgccc gacgtcatcg tgatgaaggt ggcgcgccag tggaaggccg
     9541 tgctcaaccc ggacgtgatg cccaagctgc gcgtcaacga cctttacgcg caggccatca
     9601 agtcgcagaa gggcagcggc agccagctgt cggcgcggct gcaggaggcg cgctggttca
     9661 tccgcaacat ccagcagcgc ttcgacacca tcctgcgcgt gtcgcaggcc atcatcgagc
     9721 ggcagaagaa cttcttctcg cacggcgcca tcgcgatgaa gccgctggtg ctgcgcgaga
     9781 tcgccgatga actgggtctg cacgaatcca cgatctcgcg cgtgaccacc gccaagtaca
     9841 tggccacgcc gttcggcacc tacgagctga agtacttctt cggctcgtcg ctgaacacgg
     9901 acgccggcgg caacgcgtcg tcgaccgcgg tgcgcgcgct catcaagcag ctggtggccg
     9961 ccgagagccc cgccaagccg ctgtccgaca gccagctcgc cgagatgctc gagggccagg
    10021 gcatccaggt cgcgcggcgc accgtcgcca agtatcgtga agccctgaag atcgcgccgg
    10081 cgaacctgcg ccgcacggtc tgacctttct cccacgcgtc cgcgaggagc gggcgttccc
    10141 cgacgtgccg aagggtgcac tgccatgacc accgatttcc ttttcgtcac ctgcgccaac
    10201 ggcagcgaac agctgctcgc cgaggagtgc gcgcgcatcc tgggcaccac ccagggcatc
    10261 gaggccggcc gcggcggcgt gatggtcgac gagaacgtgg aacacgcgat gcgcctgaac
    10321 ctgcacagcc gcctcgcgca acgggtgctc tggccgatgg tggacggcga gtaccgtgac
    10381 gagcgcgacc tgtacg
//
LOCUS       DATCRO010000463         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_927520, whole genome shotgun sequence.
ACCESSION   DATCRO010000463 DATCRO010000000
VERSION     DATCRO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927520"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VIN75_12685"
     CDS             <1..497
                     /locus_tag="VIN75_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8975071.1"
                     /translation="VEVARTLMRSDAASIQELDAADSRLKLLAHTGFHPESAAYWAYV
                     DADVGTSCGRALTARKRVVVPDMDQFDADAHDIAAYRKSGIMSVQSTPLVASSGHIVG
                     MMSTHWNHAHAPSAESYRFFDVLVRLAADFMERARARTALCDSEARYRSLFETTRRGC
                     IEND"
     gene            complement(565..1122)
                     /locus_tag="VIN75_12690"
     CDS             complement(565..1122)
                     /locus_tag="VIN75_12690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8975072.1"
                     /translation="MRENGLLAIGDPRSNGRFGVLKNTALGTCIESPGKGWRSMTLRI
                     SGTTAIKAAACVLSLHVCVPAHGQASQAASAPASASGEGTQGRKTCRPTYPEAAKRAR
                     AEGTTVVQLTVAADGAATKVVVARSAGPTPEHKMLDQAAVEALRACPFKPAADATGKP
                     VEATVKVDYVWLLDPPGAAASGARR"
     gene            1157..3268
                     /gene="ligA"
                     /locus_tag="VIN75_12695"
     CDS             1157..3268
                     /gene="ligA"
                     /locus_tag="VIN75_12695"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8975073.1"
                     /translation="MTATTPDTARKRADELHEILHEHAYRYYVLDEPTIPDAEYDRLF
                     QELQAIEQQHPELARPDSPTQRVIGQVLEGFEPVRHAVPMLSIRTETDTTAGGAEAFD
                     ARVRRELKLTDADPPVAYSAELKFDGLAINLRYEHGVLVQAATRGDGEVGEDVTSNIR
                     GIKQIPLRLRGVDAPVLEVRGEAYMRRDDFEAYNERQRALIAAGAKNEKTVVNPRNAA
                     AGAVRQLDSAQSRKRPLSFFAYGLGDVRGWMVPPTHSGLLDALAAMGLPVARQRIVSE
                     GAAGLVAFHRKIAAERDQLPFDIDGVVYKVDSRELQARLGFVSREPRWAVAHKYPAQE
                     QMTTLLDIDVQVGRIGTLTPVARLQPVFVGGTTVSNATLHNEDEIRRKDIRIGDTVIV
                     RRAGDVIPQVVGPILEKRPPEVAALAPFDMFAHVGGKCPVCASPIERREGEVAWRCTG
                     GVTCPAQVKQAMLHFAGRRAVEIEGLGDELVEQLVDAGIVRTLPELYKLGFAKLVALE
                     RMGDKSAQNLVQAIEKSKSTTLPRFLFGLGIRHVGETTAKDLARHFGDIDKLMDASVE
                     QLLEVPDVGPIVASSVRAFFDQPHHREIVEQLRAAGLHWPAIEGTASDAHRPLLGKTL
                     VITGTLPTLSRDEAKDLIEAAGGKVSGSVSKKTNWLVAGEEAGSKLEKAQSLGVAVID
                     EAWLQALLAAPADAIDAAAGE"
BASE COUNT          522 a         1189 c         1159 g          462 t
ORIGIN      
        1 tcgtggaggt ggcgcgcacg ctcatgcgtt cggatgccgc gagcatccag gaactggatg
       61 cggccgattc gcggctgaaa ctgcttgcgc ataccggctt tcacccggaa tcggctgcgt
      121 actgggccta tgtcgatgcc gatgtcggca cttcctgcgg acgcgcgctg accgcgcgca
      181 aacgcgtcgt cgtgccggac atggatcaat tcgatgcgga tgcccacgat atcgcggcct
      241 accgcaaatc cggaatcatg tcggtgcaat cgacgccgct ggtcgccagc agcggacaca
      301 tcgtcggcat gatgtccacc cactggaacc acgcgcacgc accgagcgcc gaaagctatc
      361 gcttcttcga cgtcctggtg cggctcgccg ccgacttcat ggaacgcgcc cgcgcccgga
      421 cggccctgtg cgacagcgag gcgcgctacc gcagcctgtt cgagacgacg cgacggggct
      481 gcatcgagaa cgattgatcc gcggctccga tggcgggcgc cgtgacatgg tcggccgcca
      541 tcgtcgcggc gccaggccct tgcatcagcg tcgagcgccg gacgccgccg cacccggcgg
      601 gtcgagcaac cacacatagt cgaccttgac ggtcgcctcc acgggcttgc cggtcgcatc
      661 ggccgcaggc ttgaacgggc aagcccggag tgcctcgacc gcggcctggt cgagcatctt
      721 gtgctcgggg gtggggccgg cgctcctggc gaccacgacc ttcgtcgccg cgccatccgc
      781 cgcgacggtc agttgcacga cggtcgtgcc ctcggccctg gcgcgttttg ccgcttccgg
      841 ataggtcgga cggcacgtct tcctgccctg ggtcccctcg ccggatgccg atgcgggcgc
      901 cgacgcggct tgcgaggcct ggccgtgggc cggcacgcac acgtgcagcg agagcacgca
      961 agccgccgcc ttgatggccg tggtgccgga tattcgcagg gtcatgctcc tccatccctt
     1021 gcctggcgac tcgatgcacg tccccaaagc cgtgttcttc agcacgccga accgcccgtt
     1081 cgatcgcgga tcgccgatcg cgagcaggcc attctcgcgc aaggcgtccg ccgcccggcg
     1141 cgcctacact cacgcgatga ccgcgacgac ccccgacacc gcccgcaagc gcgccgacga
     1201 gctgcacgag atcctgcacg agcacgcgta ccgctactac gtgctggacg agcccaccat
     1261 ccccgacgcc gaatacgacc ggctgttcca ggagctccag gcgatcgagc agcagcatcc
     1321 cgaactggcg cggcccgatt cgccgacgca gcgcgtcatc ggccaggtgc tcgaaggctt
     1381 cgagcccgtg cgccacgccg tgcccatgct cagcatccgc accgagaccg acaccaccgc
     1441 cggcggcgcc gaggcgttcg acgcgcgcgt gcgccgcgag ctcaagctca ccgacgccga
     1501 tccgccggtg gcctattccg cggagctcaa gttcgacggc ctggccatca acctgcgcta
     1561 cgagcacggc gtgctggtgc aggccgccac gcgcggcgac ggcgaggtgg gcgaggacgt
     1621 cacgtccaac atccgcggca tcaagcagat cccgctgcgc ctgcgcggcg tcgatgcgcc
     1681 ggtgctcgag gtgcgcggcg aggcctacat gcggcgcgac gacttcgagg cctacaacga
     1741 gcgccagcgc gcgctgatcg ccgccggcgc gaagaacgag aagaccgtcg tcaacccgcg
     1801 caacgccgcg gccggcgcgg tgcgccagct cgattcggcg cagtcgcgca agcggccgct
     1861 gagcttcttc gcgtacggcc tgggcgacgt ccggggctgg atggtgccgc ccacgcattc
     1921 cgggttgctc gatgcgctgg cggcgatggg actgccggtg gccaggcagc gcatcgtcag
     1981 cgagggcgcc gcgggcctcg tggccttcca ccggaagatc gccgccgagc gcgaccagct
     2041 gccgttcgac atcgacggcg tcgtctacaa ggtcgactcg cgcgagctgc aggcccggct
     2101 gggcttcgtc agccgcgagc cgcgctgggc ggtcgcgcac aagtacccgg cgcaggagca
     2161 gatgaccacg ctgctggaca tcgacgtgca ggtcggccgc atcggcacgc tcaccccggt
     2221 ggcgcggctg cagccggtgt tcgtcggcgg cacgaccgtc agcaacgcca cgctgcacaa
     2281 cgaggacgag atccgccgca aggacatccg catcggcgac accgtgatcg tgcggcgcgc
     2341 cggcgacgtc atcccgcagg tcgtcggccc catcctggaa aagcgcccgc ccgaggtcgc
     2401 cgcgctcgcg ccgttcgaca tgttcgcgca cgtcggcggc aagtgcccgg tgtgcgcgag
     2461 ccccatcgag cggcgcgagg gcgaggtcgc ctggcgctgc acgggcggcg tcacgtgccc
     2521 ggcgcaggtc aagcaggcga tgctgcactt cgccggacgg cgcgcggtcg agatcgaagg
     2581 cctgggcgat gagctggtcg agcagctggt cgacgccggc atcgtgcgca cgctgcccga
     2641 gctctacaag ctgggctttg ccaagctcgt ggcgctcgag cgcatgggcg acaagagcgc
     2701 gcagaacctg gtccaagcga tcgagaagag caagtccacc acgctgccgc gcttcctgtt
     2761 cgggctgggc atccgccatg tcggcgagac gacggccaag gacctggcgc gccacttcgg
     2821 ggacatcgat aagctgatgg acgcgagcgt cgagcagttg ctggaggtgc ccgacgtcgg
     2881 ccccatcgtc gccagcagcg tgcgcgcgtt cttcgatcag ccgcaccacc gcgagatcgt
     2941 ggagcagcta cgcgcggcgg gcctgcactg gcccgccatc gagggcaccg ccagcgacgc
     3001 gcaccgcccg ctgctgggca agacgctggt gatcacgggc acgctgccca ccctcagccg
     3061 tgacgaggcc aaggacctga tcgaggccgc cggcggcaag gtcagcggct cggtgtccaa
     3121 gaagacgaac tggctcgtgg ccggcgagga ggccggcagc aagctggaga aggcgcagtc
     3181 gctcggcgtg gccgtcatcg acgaggcctg gctgcaggcg ctgctggcgg cgccggcgga
     3241 cgcgatcgat gcggcagcgg gcgagtgagc ggcacggccg tcgcgtgcat ccgtgcggcg
     3301 ggcgacaatc catggccctg cagatcgaac gg
//
LOCUS       DATCRO010000464         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_736558, whole genome shotgun sequence.
ACCESSION   DATCRO010000464 DATCRO010000000
VERSION     DATCRO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736558"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VIN75_12700"
     CDS             <1..509
                     /locus_tag="VIN75_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975074.1"
                     /translation="SAQVAAATPDASAPAPRRATPARDAPAPASAPPRIARVRPPGTD
                     QSPDITDYINDGEKPTMKDVIARLNAAGVHTGLGAFNPPGTRPPLVGLAVPEDFALPP
                     GYVRHYQATDDGQRIEPILMFAPDFQPATADGHPVPLPKDRVVPPELAPPGLPIRRIV
                     VPAPIEGQ"
     gene            514..>2540
                     /gene="lnt"
                     /locus_tag="VIN75_12705"
     CDS             514..>2540
                     /gene="lnt"
                     /locus_tag="VIN75_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8975075.1"
                     /translation="MTTSADFLSARPRTRDAAAVAAGALLAFAYARGGAGWVLGFLAL
                     VPWLRALDVPGPLARTLLLAWAMTVAYTLAVFGWFGGAIGQFTQWGEPAGLALLLLAA
                     PLFQPQILAFAIARRVAAREHGRARAAIVAAAAWVATEWAVPRLLGDTLGHGLYPSRL
                     LRQAADLGGATGLTLALLLANEAVAAALARRHAGRRAQLRPLALAASVPALLAIYGAV
                     ALAGLPAPAGKPLRVGLVQASIVDYERLRQEIGAANVVRRVLDTHFAMSYDAVERQHA
                     DAVLWSETVYPTTFGHPKSEAGAALDQEIRSIVNAAGVPFVFGTYDVDAAGEYNAAAF
                     VEPNHGLLGFYRKTRLFPLTEYVPPWLDGPALRRWLPWAGTWKPGSGARLLPLRLADG
                     REIPALPLICLDDVDPALAIDGARLGGQVILAMSNDAWFTRHPQGAALHEAVAAFRSI
                     ETRLPQFRVTTNGYSAVIDATGRVLAGTRMGAQALAVGDLPVRTPPRTLMVAWGDWVG
                     RAAAALLALLAALALLRRRGWTALGAPAPAADVPALPVDVALLPRAARVAAGALRALA
                     RGGLLWMIAAVALGDVALQSNTLAQIRAFTGLFLVPEAAAWCVLRAFAAHASIADGAL
                     VFTRGARRIALPLADIAAVEPWSLPVPGPGAALRLRSGARWPHGLALAHPQG"
BASE COUNT          259 a         1044 c          904 g          333 t
ORIGIN      
        1 cgtccgcgca ggtcgccgcc gcgacgcccg acgcgtcagc gccggccccc aggcgcgcga
       61 cgcccgcgcg tgacgcgccc gcgcccgcga gcgcgccccc gcgcatcgcc cgcgtgcgcc
      121 cgccgggcac cgaccagtcg cccgacatca ccgactacat caacgacggc gagaagccga
      181 cgatgaagga cgtcatcgcc cggctcaacg ccgcgggcgt ccacaccggc ctgggcgcgt
      241 tcaacccgcc cggcaccagg ccgccgctcg tgggcctggc cgtgcccgag gacttcgcgt
      301 tgccgcccgg ctacgtgcgc cactaccagg cgaccgacga cggccagcgc atcgagccga
      361 tcctgatgtt cgcgcccgac ttccagccgg cgacggccga cggccatccc gtcccgctgc
      421 ccaaggatcg cgtcgtgcca cccgagctcg cgccgccggg cctgccgatc cgccgcatcg
      481 tcgttcccgc accgatcgaa ggccagtgac gccatgacca cctccgccga cttcctgtcc
      541 gcgcgcccgc gcacccgcga cgcggccgcc gtcgccgccg gcgcgctgct ggccttcgcg
      601 tacgcccgcg gcggcgccgg ctgggtgctg ggcttcctgg cgctggtgcc gtggctgcgc
      661 gcgctggacg tgcccggccc gctcgcgcgc acgctgctgc tcgcctgggc gatgaccgtc
      721 gcgtacacgc tcgcggtgtt cggctggttc ggcggcgcga tcggccagtt cacgcaatgg
      781 ggcgagccgg cggggctcgc cttgctgctc ctcgcggcgc cgctgttcca gccgcagatc
      841 ctcgcgttcg cgatcgcgcg ccgggtcgcc gcgcgcgagc acgggcgtgc gcgcgcggcc
      901 atcgtcgcgg ccgcggcctg ggtcgccacc gaatgggcgg tgccgcgcct gctcggcgac
      961 acgctgggcc acggcctgta cccgtcgcgc ctgctgcgcc aggccgccga tctcggtggc
     1021 gccaccggcc tgacgctcgc attgctgctc gccaacgagg ccgtcgcggc ggccctcgcc
     1081 cgacggcacg cagggcgtcg cgcccagctg cgcccgctcg cgctcgccgc gtcggtgccc
     1141 gcgctgctgg ccatctacgg cgccgtcgca ttggccggcc tgcccgcgcc cgccggcaag
     1201 ccgctgcgcg tcgggctcgt gcaagcgagc atcgtcgact acgaacgcct gcgccaggag
     1261 atcggcgccg cgaacgtggt gcgccgggtg ctcgacacgc acttcgcgat gtcgtacgac
     1321 gccgtcgagc gccagcacgc cgacgcggtg ctgtggtccg agacggtcta tcccaccacc
     1381 ttcggccacc ccaagagcga ggccggcgcg gccctggacc aggagatccg gtccatcgtc
     1441 aacgccgccg gcgtgccgtt cgtgttcggc acctacgacg tcgacgccgc cggcgagtac
     1501 aacgccgcgg ccttcgtcga gccgaaccac ggcctgctcg gcttctatcg caagacgcgc
     1561 ctgttcccgc tgaccgaata cgtgccgccg tggctggacg gccccgcgct gcgacgctgg
     1621 ctgccgtggg ccggcacctg gaagcccggc agcggcgcgc gcctgctgcc gctgcgcctg
     1681 gccgacggcc gcgagatccc ggcgctgccg ctgatctgcc tggacgacgt cgacccggcc
     1741 cttgcgatcg acggcgcacg cctgggcggc caggtgatcc tggcgatgtc caacgacgcc
     1801 tggttcaccc ggcacccgca gggcgccgcg ctgcacgagg cggtggccgc gttccgcagc
     1861 atcgagacgc gcctgccgca gttccgcgtg accaccaacg gctacagcgc cgtgatcgac
     1921 gccaccggcc gcgtgctggc cggcacgcgg atgggcgcgc aggcgctggc ggtcggcgac
     1981 ctgccggtgc gcacgccgcc gcgcacgttg atggtggcat ggggcgactg ggtgggccgc
     2041 gccgcggcgg ccttgctcgc gttgctggcc gcgctcgcgc tgctgcgccg ccgcggctgg
     2101 acggccctcg gtgcgcctgc gccggccgcc gacgtccccg cgctgccggt cgacgtcgcg
     2161 ctgctgccgc gcgccgcgcg cgtggccgcg ggcgccctgc gcgcgctcgc gcgcggcggc
     2221 ctgctgtgga tgatcgccgc cgtcgcgctc ggcgacgtcg cgctgcagtc gaacacgctg
     2281 gcgcagatcc gcgcattcac agggctgttc ctcgtgcccg aggccgccgc ctggtgcgtc
     2341 ctgcgcgcgt tcgccgcgca tgcctccatc gccgacggcg cactcgtctt cacgcgcggc
     2401 gcgcgccgga tcgcgctgcc gctggccgac atcgcggccg tcgaaccctg gtcgctgccc
     2461 gtccccggcc cgggcgcggc gctgcgcctg cgttcgggcg cccgctggcc gcacggcctc
     2521 gcgctcgcgc acccgcaggg
//
LOCUS       DATCRO010000465         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_185206, whole genome shotgun sequence.
ACCESSION   DATCRO010000465 DATCRO010000000
VERSION     DATCRO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185206"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1213)
                     /locus_tag="VIN75_12710"
     CDS             complement(236..1213)
                     /locus_tag="VIN75_12710"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEY8975076.1"
                     /translation="MEIFDYDNILLLPRKCVVDSRSECDTSMEFGPHRFRLPVVPANM
                     KTVLDEPIAQFLASNGYFYVMHRFDLDTVAFARTMRDKGLVVSISAGVKPEDHAVIER
                     LASTGVGADYVTIDIAHGHAESVKRMIAHIKKTLPQAFVIAGNVGTPEAVIDLENWGA
                     DATKIGVGPGKVCITRLKTGFGTGGWQLSALKWCARVATKPIIADGGIRHNGDIAKSL
                     RFGATMVMVGSLFAGHEESPGQTVEVDGKLYKEYYGSASDFNKGEYRHVEGKRILEPI
                     KGRLPDTLREMQEDLQSSISYAGGRCAADIRKVNYVILGGENAGEHLFM"
     gene            complement(1226..1975)
                     /locus_tag="VIN75_12715"
     CDS             complement(1226..1975)
                     /locus_tag="VIN75_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975077.1"
                     /translation="MHPPILGSVLHRGRLALVAAGLLASALALAAPKPPPGVAQNCPA
                     RATLLVEHFIGADCLDCWQAAPPGLGAMPVGPREWSLDWIAPTADDAPMAPGALPEAG
                     ERLARINAELPARIASAPSAFDSVTALPRSAPKRRFSVHSSLPHNGYMGVQMHATGVW
                     PAGSTGWIALVELIDPGVRGTQIPRRLVRVLVGPVKLPAGPGARNAVAPLYGLRWPEN
                     AHAENLVATAWVEDAAGRITQVATDRCADPR"
     gene            1970..4363
                     /locus_tag="VIN75_12720"
     CDS             1970..4363
                     /locus_tag="VIN75_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8975078.1"
                     /translation="MHIQHSDAEPEAPGADRLLRGLNAEQLAAVTLPARPALILAGAG
                     SGKTRVLTTRIAWLLQTGQASPGGLMAVTFTNKASKEMLARLSAMLPINVRGMWIGTF
                     HGLCNRFLRAHWKLAGLPQGFQILDSSDQLSAVKRIIKAMQLDEERFVPKQVTWFIAG
                     AKEDGLRPKDVEIHDGQTQKMVEIYQAYEDQCNREGVVDFAELLLRAFELMRDNEALR
                     EHYRHRFQHILVDEFQDTNRLQYAWLKMFAGPHGAVFAVGDDDQSIYGFRGALVGNMA
                     DFEREFRVDKVIKLEQNYRSFGNILDAANHLIDRNSKRLGKNLRTDQGPGEPVRVFES
                     TSDFAEAQWFLDEAQQLHRAGTARSEIAVLYRSNAQSRVIESALFNAGIPYRVYGGLR
                     FFERAEVKHALSYLRLIENPNDDTSFLRVVNFPARGIGARTIELLQDSARQTGRSLFQ
                     SVGSVAGKGGANLAAFTGLVQRMQDATRGMTLRDIIEHMLAETGLIDFYRADKDGADR
                     VENLEELMNAAEAFVTQEGFGRDAVALPVDELSPGSLARRDTSRDVPGLVLDAPTTPD
                     AETGEIMSPLAAFLTHASLEAGDNQAQAGQDAIQLMTIHSAKGLEFDSVFITGLEEGL
                     FPHENSLQSNDGLEEERRLMYVAITRARKRLYLSFSQTRLLHGQTRYNVKSRFVDELP
                     EANLRWLTPRNQAFGSGYSREYKQAWERGSGLGSMIGAGRVAGSPPVPKAMQDSRTAS
                     SNGHQLRQGQSVFHTKFGEGVIVTLEGSGDEARAQVNFGRHGMKWLALSVAKLTPVD"
     gene            4557..>4891
                     /locus_tag="VIN75_12725"
     CDS             4557..>4891
                     /locus_tag="VIN75_12725"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975079.1"
                     /translation="MTPLFDANLRLVAFFDGTYVFDTDNEWVAFADRGHAFARGGRWL
                     GPLDSGTFQDQDGLAVAWLAGSQPTTGMKPARPMNPRLPLHPKRPLRPRMPLPPPYPL
                     QPGGGWSKLT"
BASE COUNT          753 a         1724 c         1695 g          719 t
ORIGIN      
        1 acgatggccg gcgcgtagtc gatcaggaac gccagcagcc gcgtgccggg ctcggccagc
       61 accaggctgt ccgatggcgc cggatcggcg accagggcgc gcggcggcgc gaagcggttc
      121 gaagaattct cgttgttgtc gtccatggcc ggttcctccc ctttggctgt cgagttgacg
      181 ccgggaatgc tagcgcagcc ggccgtcgcg ccgcctcggt atcggcccgg acgcgtcaca
      241 tgaacaggtg ttcacccgcg ttctcgccgc ccaggatcac gtagttgacc ttgcggatgt
      301 cggcggcgca gcggccgccg gcgtacgaga tcgagctctg gaggtcttcc tgcatctcgc
      361 gcagggtgtc gggcagccgg cccttgatcg gctcgaggat gcgcttgccc tcgacgtggc
      421 ggtactcgcc cttgttgaag tcggacgcgc tgccgtagta ctccttgtag agcttgccgt
      481 cgacctccac cgtctgcccc gggctctcct cgtggccggc gaacagtgag ccgaccatca
      541 ccatcgtggc gccgaagcgc aggctcttgg cgatgtcgcc gttgtggcgg atgccgccgt
      601 ccgcgatgat gggcttggta gccacgcggg cgcaccactt gagcgccgac aactgccagc
      661 cgccggtgcc gaagcccgtc ttgagccgcg tgatgcagac cttgccgggg cccacgccga
      721 tcttggtggc gtcggcgccc cagttctcca ggtcgatgac cgcctcgggc gtgccgacgt
      781 tgccggcgat cacgaaggcc tgcggcagcg tcttcttgat gtgggcgatc atgcgcttga
      841 cgctctcggc gtggccgtgc gcgatgtcga tggtgacgta gtcggcgccg acgcccgtgg
      901 acgcgaggcg ctcgatgacg gcatggtcct cgggcttgac gcccgcgctg atcgacacca
      961 cgaggccctt gtcgcgcatg gtcctcgcga aggcgacggt gtccaggtcg aaccggtgca
     1021 tcacgtagaa atagccgttg ctcgcgagga actgcgcgat cggctcgtcg agcaccgtct
     1081 tcatgttggc cggcaccacc ggcagcctga agcggtgcgg gccgaactcc atcgaggtgt
     1141 cgcactcgct gcggctgtcg accacgcact tgcgcggcag cagcaggatg ttgtcgtagt
     1201 cgaagatttc catgatggac tttcgttatc tcggatcggc gcagcggtcg gtggcgacct
     1261 gcgtgatgcg gccggcggcg tcctccaccc aggcggtggc gaccaggttc tcggcgtgcg
     1321 cgttctcggg ccagcgcagg ccgtacagcg gcgcgacggc attcctcgcg ccggggccgg
     1381 ccggcagctt gacagggccc accagcacgc gcaccaggcg gcgcgggatc tgcgtgccgc
     1441 gcacgcccgg gtcgatcagc tcgaccagcg cgatccagcc ggtgctgccc gcgggccaca
     1501 cgccggtcgc atgcatctgc acgcccatgt agccgttgtg cggcaggctg gagtgcacgg
     1561 agaagcgccg tttcggcgcg ctgcggggca acgccgtgac gctgtcgaac gccgacggcg
     1621 cgctggcgat gcgcgcgggc agctccgcgt tgatgcgcgc caggcgctcg ccggcctcgg
     1681 gcagcgcgcc cggcgccatc ggggcgtcgt cggcggtggg cgcgatccag tccaggctcc
     1741 attccctggg gccgacgggc atcgcgccca ggcccggcgg cgccgcctgc cagcaatcga
     1801 ggcagtcggc gccgatgaag tgctcgacca gcagcgtggc cctggccggg cagttctgcg
     1861 ccacgccggg cggcggcttg ggcgcggcca gggccagcgc agaggccagc agcccggcgg
     1921 cgaccagggc aagccggccg cgatgcagga cagaacctaa aatgggtgga tgcatatcca
     1981 gcattctgac gccgaacccg aagccccggg cgccgaccgg ttgctccgcg gcctgaacgc
     2041 cgagcaactg gccgcggtga ccctgcccgc gcggcccgcg ctcatcctgg ccggcgccgg
     2101 atcgggcaag acgcgcgtgc tcaccacgcg catcgcctgg ctgctgcaga ccggccaggc
     2161 ctcgcccggc ggcctgatgg cggtcacctt caccaacaag gcgtccaagg agatgctggc
     2221 gcggctgtcg gcgatgctgc cgatcaacgt gcgcggcatg tggatcggca ccttccacgg
     2281 cctgtgcaac cgcttcctgc gcgcgcactg gaagctggcc ggcctgccgc agggcttcca
     2341 gatcctcgat tcgtccgacc agctgtcggc cgtcaagcgc atcatcaagg cgatgcagct
     2401 cgacgaggag cgcttcgtgc ccaagcaggt gacgtggttc atcgccggcg cgaaggagga
     2461 cggcctgcgc ccgaaggacg tggagatcca cgacgggcag acgcagaaga tggtcgagat
     2521 ctaccaggcg tatgaagacc agtgcaaccg cgagggcgtg gtcgacttcg ccgagctgct
     2581 gctgcgcgcc ttcgagctga tgcgcgacaa cgaggcgctg cgcgagcact accgccatcg
     2641 cttccagcac atcctcgtcg acgagttcca ggacaccaac cgcctgcagt acgcctggct
     2701 gaagatgttc gcgggcccgc acggcgcggt gttcgcggtg ggcgacgacg accagagcat
     2761 ctacggcttc cgcggcgcgc tggtgggcaa catggccgac ttcgagcgcg agttccgcgt
     2821 cgacaaggtc atcaagctgg agcagaacta ccgcagcttc ggcaacatcc tggatgcggc
     2881 gaaccacctg atcgaccgca actccaagcg cctgggcaag aacctgcgca ccgaccaggg
     2941 ccccggcgag ccggtgcgcg tgttcgagtc gacgagcgac ttcgccgagg cacagtggtt
     3001 cctcgacgag gcgcagcagc tgcaccgcgc gggcacggcg cgctccgaga tcgcggtgct
     3061 ctatcgcagc aacgcgcagt cgcgcgtcat cgagtcggcg ctgttcaacg ccggcatccc
     3121 gtaccgcgtg tacggcggcc tgcgcttctt cgagcgcgcc gaagtcaagc acgcgctgtc
     3181 gtacctgcgg ctgatcgaga acccgaacga cgacaccagc ttcctgcgcg tcgtcaactt
     3241 cccggcgcgc ggcatcggcg cgcgcaccat cgagctgctg caggactcgg cgcgccagac
     3301 cggccgcagc ctgttccaga gcgtgggcag cgtggccggc aagggcggcg cgaacctggc
     3361 cgcgttcacg ggcctcgtgc agcgcatgca ggacgccacg cgcggcatga cgctgcgcga
     3421 catcatcgag cacatgctgg ccgagaccgg cctgatcgac ttctaccgcg ccgacaagga
     3481 cggcgccgac cgcgtcgaga acctggagga actgatgaac gccgccgagg ccttcgtcac
     3541 ccaggagggc ttcggccgcg acgcggtggc gctgccggtc gacgagctgt cgcccgggtc
     3601 gctcgcccgg cgcgacacct cgcgcgacgt gcccggcctc gtgctggacg cgccgaccac
     3661 gcccgacgcc gagaccggcg agatcatgtc gccgctggcc gcgttcctga cccacgcatc
     3721 gctggaggcc ggcgacaacc aggcccaggc gggccaggac gccatccagc tgatgaccat
     3781 ccattcggcc aagggcctgg agttcgattc ggtcttcatc accgggctgg aagagggcct
     3841 gttcccgcac gagaactcgc tgcagtcgaa cgacggcctc gaggaggagc gccgcctgat
     3901 gtacgtggcg atcacgcggg cccgcaagcg cctgtacctg agcttcagcc agacgcgcct
     3961 gctgcacggc cagacgcgct acaacgtcaa gagccgcttc gtcgacgagc tgcccgaggc
     4021 caacctgcgc tggctcacgc cgcgcaacca ggccttcggc tcgggctact cgcgcgagta
     4081 caagcaggcg tgggagcgcg gctcgggcct gggctcgatg atcggcgccg gccgcgtggc
     4141 cggcagcccg ccggtgccca aggcgatgca ggacagccgc accgcgtcca gcaacggcca
     4201 ccagctgcgc cagggccagt cggtcttcca caccaagttc ggcgagggcg tgatcgtcac
     4261 gctggaaggc tccggggacg aggcgcgcgc gcaggtcaac ttcggccgcc acggcatgaa
     4321 gtggctggcg ctgtcggtgg ccaagctgac gccggtggat tgaaacgtcg cccgcgtgcg
     4381 tggatcctgc gactgcgcgc aggatggcgg ctcagcgcac ccaggccgcc cgtcaaacga
     4441 ggggtcggct ggcctcgacc gtcagtccct gcgtcggcca aactacagcg tcatcctgcg
     4501 cgcagcgaag cgaagtcgca ggatccacct ccgccaaccc agcatcgagc tccgagatga
     4561 cgcccctgtt cgacgccaac ctgcgcctcg tcgccttctt cgacggcacc tacgtcttcg
     4621 acaccgacaa cgagtgggtc gcgttcgccg accgcggcca cgcgttcgcg cgcggcgggc
     4681 gctggctcgg accactggac tccggcacgt tccaggacca ggacggcctg gccgtggcct
     4741 ggctggccgg atcgcagccg accaccggca tgaaacccgc gcggccgatg aacccgcgcc
     4801 tgccgctgca ccccaagcgc ccgctgcgtc cgcgcatgcc gctgccgccg ccgtatccgc
     4861 tgcagccggg cggcggatgg tcgaagctga c
//
LOCUS       DATCRO010000466         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_511693, whole genome shotgun sequence.
ACCESSION   DATCRO010000466 DATCRO010000000
VERSION     DATCRO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511693"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VIN75_12730"
     CDS             complement(<1..591)
                     /locus_tag="VIN75_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975080.1"
                     /translation="MRSSTELIRLAVLAAACAAGGARAQDAAAPPPVAESAAPAPATD
                     GEQRAARIEKAVDELRADPLLSGKHMEKRPRWIDDDDEPRKKKKLEADSSLFGWLAAL
                     GRFLSDTSRFLLWGGIAVLVAILLVSARHLVNLRAFERRAKAEAAVSHVRDLDVRPES
                     LPDDVGAAAWALWQAGRVPEAMSLLYRGALSRLIHKY"
     gene            complement(575..1306)
                     /locus_tag="VIN75_12735"
     CDS             complement(575..1306)
                     /locus_tag="VIN75_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975081.1"
                     /translation="MKAEQLQLALRPRPAYEAVDLGVRMAQRSGRSLVRAFLPFGLLL
                     VVACGATIEVAWWLPSTLLWWFKPWLDRTLLDIYARHAFGDETTFADAWEARRHAPWI
                     PMLEWLTIWRLSFWRSYSMPVGQLEGQTGSARRQRVKAILRAHRGSATMMQGTFSWIE
                     FFLSSGLVALIPWMTPGLHPFGDIEWLSSDAIATKLLLYAAHASAVLFVEPFFVASGF
                     AMYLNRRVELEAWDVEQDLRHAFEH"
     gene            complement(1303..2328)
                     /locus_tag="VIN75_12740"
     CDS             complement(1303..2328)
                     /locus_tag="VIN75_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY8975082.1"
                     /translation="MTPRQFEARYEPAWVELERGLNALDAGRKRRKAGPFETGTNEVP
                     TAARVAQLYRQCCEHLALARERAYPVHLVARLEGLTARAHQRIYRRHDFGLAAFRHLV
                     LYKAPAAVRALRWHLLVATLVFVLPTIVVGVATWLDPHFVLTVVDARQVHEFDGMYGQ
                     QAGEHLGRTSGDDWTMFGFYIMNNVGIAFRCFAAGLVLGVGSLFMVGYNGLLAGAVAG
                     FLVTRGDSERFFSFVVTHSAFELTAIVLSGAAGLKLGHAMLAPGRLTRTQALMRAARE
                     TSPVVFCFTMMLFIAAAIEAFWSSAGWVAPGVKYAVGACCWTLVFAYLGLQGKGHDAA
                     LVEARAR"
BASE COUNT          379 a          853 c          855 g          289 t
ORIGIN      
        1 gtacttgtgg atcagccgtg acagcgcacc gcggtacagc agcgacatcg cctcgggcac
       61 gcgccccgcc tgccacagcg cccacgcggc ggcgccgacg tcgtcgggca ggctctcggg
      121 ccgcacgtcg aggtcgcgca cgtggctcac ggcggcctcg gccttcgcac ggcgctcgaa
      181 ggcgcgcagg ttgaccaggt ggcgcgcgct caccagcagg atcgccacca gcaccgcgat
      241 gccgccccaa agcaggaagc ggctggtgtc gctgaggaag cggccgagcg cggccagcca
      301 cccgaacagc gacgagtcgg cctccagctt tttcttcttc ctcggctcgt cgtcgtcgtc
      361 gatccagcgc ggccgcttct ccatgtgctt gcccgacagc agcggatcgg cgcgcagttc
      421 gtcgacggcc ttctcgatgc gcgcggcgcg ctgttcgccg tcggtggcgg gcgccggcgc
      481 ggcgctctcg gccacgggcg gcggcgccgc ggcgtcctgc gcgcgggcgc cgccggccgc
      541 gcaggcggcg gccagcacgg caaggcggat cagttcagtg ctcgaacgca tggcgcaggt
      601 cctgctcgac atcccaggcc tcgagttcga cgcggcgatt gaggtacatc gcgaagcccg
      661 aggcgacgaa gaacggctcg acgaagagca cggcgctcgc gtgggccgcg tacagcagca
      721 gcttggtggc gatggcgtcg gagctcagcc actcgatgtc gccgaaagga tgcaggccgg
      781 gcgtcatcca gggaatcagc gcgaccagcc ccgacgacag gaagaactcg atccagctga
      841 acgtgccctg catcatcgtg gccgagccgc ggtgcgcgcg caggatcgcc ttgacgcgct
      901 ggcgccgcgc ggagccggtc tggccctcga gctggcccac cggcatcgaa tacgagcgcc
      961 agaacgacag ccgccagatc gtcagccatt ccagcatcgg gatccacggc gcgtggcggc
     1021 gcgcctccca ggcgtcggcg aacgtcgtct cgtcgccgaa cgcgtggcgc gcgtagatgt
     1081 ccagcagggt gcggtcgagc cagggcttga accaccacag cagcgtcgag ggcagccacc
     1141 aagcgacctc gatcgtggcg ccgcaggcca ccaccagcag caggccgaac ggcaggaacg
     1201 cgcgcacgag cgagcggccg ctgcgctggg ccatgcgcac gcccaggtcg acggcctcgt
     1261 aggccgggcg cgggcgcagc gcgagctgga gctgttcggc cttcatcgcg cgcgcgcctc
     1321 caccagggcg gcgtcgtggc ccttgccctg caggcccagg taggcgaaga cgagcgtcca
     1381 gcagcaggcg cccaccgcgt acttcacgcc gggcgccacc cagccggccg aggaccagaa
     1441 ggcctcgatc gccgcggcga tgaacagcat catggtgaag cagaacacca cgggcgaggt
     1501 ctcgcgcgcc gcgcgcatga gcgcctgcgt gcgcgtgagg cgcccgggcg cgagcatcgc
     1561 gtggcccagc ttcaggccgg ccgcgccgga cagcacgatg gcggtgagct cgaacgccga
     1621 gtgcgtcacc acgaacgaga agaagcgctc ggagtccccg cgcgtcacga ggaagcccgc
     1681 caccgcgccg gccagcaggc cgttgtagcc caccatgaac aggctgccga cgcccaggac
     1741 caggccggcc gcgaagcaac ggaacgcgat gccgacgttg ttcatgatgt agaagccgaa
     1801 catcgtccag tcgtcgcccg aggtgcggcc caggtgctcg ccggcctgct ggccgtacat
     1861 gccgtcgaat tcgtgcacct gcctggcgtc gacgacggtc agcacgaagt gcggatccag
     1921 ccacgtggcc acgcccacga cgatggtggg cagcacgaac acgagcgtgg ccaccagcag
     1981 gtgccagcgc agcgcgcgca cggcggcggg ggccttgtac agcaccaggt ggcggaacgc
     2041 ggccaggccg aagtcgtggc ggcgatagat gcgctggtgg gcgcgcgcgg tgagcccctc
     2101 caggcgcgcg accaggtgaa cgggataggc ccgttcacgc gccagcgcca ggtgctcgca
     2161 gcactggcgg tacaactgcg cgacgcgtgc cgccgtcggc acctcgttcg tgccggtctc
     2221 gaacggcccg gccttgcggc gcttgcggcc ggcgtcgagc gcgttcaggc cgcgctcgag
     2281 ttcgacccac gcgggctcgt agcgggcttc gaattgacgc ggagtcatcg ccatggccgc
     2341 cccgccgccc gaaggccatg gccgccccct cggggg
//
LOCUS       DATCRO010000467         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_185233, whole genome shotgun sequence.
ACCESSION   DATCRO010000467 DATCRO010000000
VERSION     DATCRO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185233"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..958
                     /locus_tag="VIN75_12745"
                     /pseudo
     CDS             173..958
                     /locus_tag="VIN75_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
     gene            complement(1055..1627)
                     /locus_tag="VIN75_12750"
                     /pseudo
     CDS             complement(1055..1627)
                     /locus_tag="VIN75_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(1806..2450)
                     /locus_tag="VIN75_12755"
     CDS             complement(1806..2450)
                     /locus_tag="VIN75_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8975083.1"
                     /translation="MKDDLRDLETRWELAIRTAGFGVWDLDPVAQTVHYPPQWKAMLG
                     YNDRDERDSTATWRARVHPDDLAPMLQALGDHLEGRSPAYEMEFRLRAADGQYRVVLS
                     RGRVVARDASGRALRVVGTLTDLTDRREAERRRLDLDRAEAASRAKLTVLGHVSHDLR
                     TPLNAVLGFAQLLRPDLDAPGMERQREYLAGIERAGWELLEHIERMLQVCERDA"
BASE COUNT          362 a         1040 c         1029 g          374 t
ORIGIN      
        1 cctttcccgc ggccaaggcc gccgcccggg tgtgcgccgg gagacaatcc gggcctcagc
       61 agatcgaacg gagcccgccg tgcacgcact cgtcttccat gaattcggcg gcgccgacgt
      121 cctgcgccac gagcagcggc ccgacccagt gcccggccca gcgcaggcgc tcgtgcgcat
      181 gcgcgcgatc ggcctgaact tcgcggacgt ctatcgccgc aagggcacct accacctggc
      241 ggggacaccg ccgtggatcc tcggctacga aggcgcgggc gaggtggtgt cggccccggc
      301 gggctccggc ctgcaggcgg ggcaacgcgt gggcttcgcc gacagcccgt tctcgaacgc
      361 ggagctgtgc gcggtcgacg tcgaccgcct ggtcccgctg ccggacgaca tcgccttcga
      421 gaccgcggcc gcgtgcctgc tgcaaggcct gacggcgcag tacctgtgcc gcgacagcca
      481 tcccgtgcgc gccggcgact gggccgtggt gcacgcggcc gcgggcggcg tggggctgct
      541 gctggtgcag atcctgcggc acctgggcgc ccgcgtcatc ggcatcgcct ccaccgagga
      601 caagcgccag gccgtgcgcg aggccggcgc cgaccacgcg gtctcgaccg agggctggct
      661 ggaccaagtg aggtcgctca ccggcggccg cggcgcggac gtcgtgtacg actccgtcgg
      721 cgcgacgctg cgcgacagcc tggacgccgc gcgcaccggc ggcaccatcg tcttctacgg
      781 catggccgcc ggcgacccgg cgccggtgga tccgcgcctg ttgatggatc gctcgctggc
      841 attggtcggc ggcgacctgt ggaacgtgct cacgaccgcg caggagcggc gcacccgcgc
      901 cgcggagctg ttcgggtgga tcgcgtcggg cgtggtgaag gtgcgcgtgt cggcgacgca
      961 tccgctgcgc gagggcgcgg cggcgcaccg ccagctcgag gggcgccggg tcgccggcgg
     1021 ccatgccgta gaagacgacg gtgccgccga cgcgggcggc gtccaggctc ccgcgcagcg
     1081 tcgcgcccac ggagtcgtac accacgtccg cgccacggcc gcccgtgagc gactgcaccg
     1141 cggccagcca gtcctcggtc gggatcacgt gctcggcgcc ggcctcgcgc acggcctggc
     1201 gcttgtcctt ggtcgacgcg atgccgatga cgcgggcgcc caggtggcgc aggatctgcg
     1261 tcaggagcag ccccaccccg cccgccgcgg cgtgcacgac ggtccagtcg ccggcgcgca
     1321 cgcggacgct gtcgcggcac aggtactgcg cggtcagccc ttgcagcagg ctggccgcgg
     1381 ccgtctcgaa ggcgatgtcg tcgggcagcg ggatcaggcg atcgacgtcg accgcgcaca
     1441 gctccgcgtt cgagaacggg ctgtcggcga agcccacgcg ctggccggcg cgcaggcccg
     1501 agcctgccgg cgccgacacc acctcgcccg cgccttcgta gccgaggatc cacggcggcg
     1561 tccccgccag gtggtagttg cccttgcggc gatagacgtc ggcgaagttc aggccgatcg
     1621 cgcgcatgcg cacgagcgcc tgcgcggggc cgggcaccgg gtcgggccgc tgttcgtggc
     1681 ggaggacatc ggcaccgccg aattcatgga agacaagcgc gtgcacggcg ggctccgttc
     1741 gatctgctga ggcccggatt gtctcccggc gcacacccgg gcggcggcct tggccgcggg
     1801 aaaggtcagg cgtcgcgctc gcagacctgc agcatgcgct cgatgtgctc cagcagttcc
     1861 cagcccgcgc gttcgatgcc ggccaggtat tcgcgctggc gctccatccc gggggcgtcc
     1921 aggtccggcc gcagcagctg cgcgaagccg agcaccgcgt tgagcggcgt gcgcaggtca
     1981 tggctgacgt gtcccagcac cgtcagcttg gcccggctgg ccgcctcggc gcgatcgaga
     2041 tccaggcggc ggcgctcggc ctcgcggcgg tcggtcaggt cggtgagcgt gcccacgacg
     2101 cgcagcgcgc gtcccgacgc atcgcgggcc accacgcggc cgcgcgacag cacgacgcgg
     2161 tactgcccgt cggccgcgcg caggcggaac tccatctcgt aggccgggct gcgcccttcg
     2221 aggtggtcac cgagggcctg cagcatcggc gcgaggtcgt ccggatgcac gcgcgcccgc
     2281 cacgtcgcgg tgctgtcgcg ctcgtcgcgg tcgttgtagc ccagcatcgc cttccactgg
     2341 ggcgggtagt gcacggtctg tgcgacggga tcgagatccc agacgccgaa accggccgtg
     2401 cgaatggcga gctcccaacg agtctcgaga tcgcgcagat cgtccttcat gacaccagac
     2461 cttgatggcg acgatcgacc cggcggctcg gcgcggcatc gacccgccgg gcggcgcgat
     2521 ccggccgggc cggactgcgc cttcttcgag gggctccctg gaatctaccg ccaatcatgc
     2581 cgctcggggc gtgcggatcg cacggcggag atcgtccgcc gacatctgtc acgacttccc
     2641 cacgccctgc ggtcgaacag ccacgatgcc gggccccgat cgtcatccgg cggccgcacc
     2701 gctcattccc gcgccgcgtc gatcgcgtcg ggaggcgccg cgagcagcgc ctgcaggccc
     2761 gcctcgtcga ggatggccac gccgagcgac tgcgccttct ccagc
//
LOCUS       DATCRO010000468         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_364986, whole genome shotgun sequence.
ACCESSION   DATCRO010000468 DATCRO010000000
VERSION     DATCRO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364986"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..1643)
                     /gene="gabT"
                     /locus_tag="VIN75_12760"
     CDS             complement(351..1643)
                     /gene="gabT"
                     /locus_tag="VIN75_12760"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY8975084.1"
                     /translation="MQREAHLNNAALMARRRAALPSGLGQSYDIVADRAENAEVWDVE
                     GKRYIDFAGGIAVLNTGHRHPKIVEAVKAQLDKVHHTCFQVLAYEPYIDLAERLIAKA
                     PGDFAKKAFFLTTGAEAVENAVKIARSATKRSAVIAFGGGFHGRTLLGMALTGKVAPY
                     KAGFGPFPAEVYHAKYPNALHGISEADALASIEGIFKYDVEPERVAAIIIEPVQGEGG
                     FYAISPSFAQALRALCDKHGIVFIADEVQTGAGRTGTWLACEQWGVAPDMITMAKSMA
                     GGFPISAVIGKASLMDAPGPGGLGGTYAGSPLACAAALAVLDVFEEEKLLERSKAIGE
                     RLVAGLKKLAGQHKAIQDVRGLGAMVAIELFKDGDLSKPDADLTKRLCAEAARRGLIL
                     LSCGSYANVVRVLVPLTASDALIDEGLGLMADSFKAVA"
     gene            1981..>2321
                     /locus_tag="VIN75_12765"
     CDS             1981..>2321
                     /locus_tag="VIN75_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8975085.1"
                     /translation="MFTLQPDSPTPLVTQIVDGLRRLIVDGSLRTGAKIPSIRHFART
                     HRVSVFTVVEAYDRLVAQGYLVSRPHSGFFVRRRAPAEAATVPGFASTASFDAQWYLR
                     SIFENRHLAVKA"
BASE COUNT          348 a          770 c          828 g          375 t
ORIGIN      
        1 ccagcttcag gccggtggcg gggtcggtga cgtcgaagcg cgcggcgccg gagacccatt
       61 cgccgccgat cagcgcgtcc gtcttcagca ggcccgcgtc gttgagcgtg gcgatggggg
      121 aagtcttcat gtccatggaa atgctcctgc ggccgggcgc gacgccctcg gatggccggt
      181 gatgatgtca actctacccg ctgcaagccg ggcgcccgcg gcggcgccgc cggggtttgc
      241 ctggggtgtg caacgaaagg aaaaaagccg cctcgatggc accggatcag tgcatcgaat
      301 ggcggctctt cgagggatgt cggcgctcgc tcgtgacggg cgcccggccc ttacgcgacg
      361 gccttgaagc tgtcggccat gaggcccagg ccctcgtcga tcagcgcgtc gctggcggtc
      421 agcggcacca gcacgcgcac gacgttggcg tacgagccgc acgacagcag gatcaggccg
      481 cgcctggctg cctcggcgca caggcgcttg gtgaggtcgg cgtcgggttt ggacaggtcg
      541 ccgtccttga acagctcgat ggccaccatg gcgcccaggc cgcgcacgtc ctggatcgcc
      601 ttgtgctggc cggccagctt cttcaggccg gcgacgaggc gctcgccgat cgccttggag
      661 cgctcgagca gcttttcttc ctcgaacacg tccagcaccg ccagcgcggc ggcacaggcc
      721 agcggcgagc cggcgtaagt gccgcccagg ccgccggggc cgggggcgtc catcagcgag
      781 gccttgccga tgacggcgga gatgggaaag ccgccggcca tggacttggc catcgtgatc
      841 atgtcgggcg cgacgcccca ctgctcgcac gccagccagg tgccggtgcg gcccgcgccg
      901 gtctgcacct cgtcggcgat gaagacgatg ccgtgcttgt cgcacagcgc gcgcagcgcc
      961 tgcgcgaacg acggcgagat ggcgtagaag ccgccttcgc cctgcaccgg ctcgatgatg
     1021 atcgcggcca cgcgctcggg ctcgacgtcg tacttgaaga tgccttcgat cgaggcgagc
     1081 gcgtcggctt cggagatgcc gtgcagcgcg ttcgggtact tggcgtggta gacctcggcc
     1141 gggaacgggc cgaagccggc cttgtagggc gcgaccttgc cggtgagcgc catgcccagc
     1201 agcgtgcggc cgtggaagcc gccgccgaag gcgatgacgg ccgagcgctt ggtggcggag
     1261 cgcgcgatct tgacggcgtt ctcgacggcc tctgcgccgg tggtcaggaa gaaggccttc
     1321 ttggcgaagt cgccgggggc cttggcgatc aggcgttcgg ccagatcgat gtagggctcg
     1381 taggccagca cctggaagca ggtgtggtgc accttgtcca gctgcgcctt gacggcctcg
     1441 acgatcttcg ggtggcggtg gccggtgttg aggacggcga tgccgccggc gaagtcgatg
     1501 tagcgcttgc cctcgacgtc ccagacctcc gcgttctcgg cgcggtcggc cacgatgtcg
     1561 tagctctggc cgaggcccga gggcagggcg gcgcggcggc gagccatcag ggcggcgtta
     1621 ttcaggtggg cttcacgttg catgagggga ctccggcgct cgcgggagcg cattgcttga
     1681 acggggacga tcgggcgcaa cccgccagac cggatgcgat gactttagtt ccggcgaccc
     1741 ggtacagcca tgtacagttg cggtgaaaca cccattggct gtacaggtcg cgctacctgt
     1801 acagcggtgt caggtcttga atcgtgcact gcacgtttca agacctgaca ccgcaacttg
     1861 acaccgcaac gttcaagacc agactgcccc aggcggtgcc aggtcttgcc tggcaacacg
     1921 cggatcgccc gttcttgcca ggcaagacct ggcacccaag acctgacacc cggacgcggc
     1981 atgttcaccc tgcaacccga ctcccccacg ccgctggtca cgcagatcgt cgacggcctg
     2041 cgccggctga tcgtcgacgg cagcctgcgc acgggcgcga agatcccgtc gatccggcat
     2101 ttcgcgagga cgcaccgggt cagcgtgttc accgtggtcg aggcgtacga ccggctggtc
     2161 gcgcagggct acctggtctc gcggccgcat tcgggcttct tcgtgcgccg ccgcgcgccg
     2221 gccgaggcgg cgaccgtgcc gggcttcgcg tcgacggcca gcttcgacgc gcagtggtac
     2281 ctgcgcagca tcttcgagaa ccgccacctg gccgtgaagg c
//
LOCUS       DATCRO010000469         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_634638, whole genome shotgun sequence.
ACCESSION   DATCRO010000469 DATCRO010000000
VERSION     DATCRO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634638"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1716)
                     /locus_tag="VIN75_12770"
     CDS             complement(10..1716)
                     /locus_tag="VIN75_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975086.1"
                     /translation="MQDGPGAPDPSPAPAPEPREDGRVCPTVGGGELRYRQLFESSPL
                     PMWVYDLETLRFLDVNEVACARYGWTRDEFLAMTILDIRPAEDAPAVRDSVRDTPAKV
                     FSSGVWRHRLKNGDIILVEITSHEMEFMGRHTRFVCPIDVTQRVNDAARLARHTEELE
                     ERVRQRTAELLVSNRELAQATAQAQQASIAKSRFLSTVSHELRTPLNAILGFAQLLTI
                     NTTQALSVTQQVSYGQHIVDAGKHLLDLINELLDLAAIEAGKTTLAIEPLQLDEVLAG
                     CTALIRPIAEPHGIELRTFTDRSMRVMSDRTRLKQVLINLLSNAVKYSPEHAVVDIAC
                     QRLGDNVRLSVIDHGAGMTPEQVDALYQPFNRLGREAGTTQGSGIGLVVTKGLVELMG
                     GRIGVDSVQGVGSTFWIELPAEPELPASVPALAAAAPSRSTSSAAISPEVTVLCVDDD
                     LANLRLIESVLATRPGVQVLTATNGRVGVELARRHLPTVIVMDNNMPEMTGREARELL
                     RADPSTAAIPIIALSGNVAGAAGAATGVEGVFRAIPKPFEVDPVLEAIDAAIAAARRR
                     AG"
     gene            complement(1928..3553)
                     /locus_tag="VIN75_12775"
     CDS             complement(1928..3553)
                     /locus_tag="VIN75_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8975087.1"
                     /translation="MAARSRPDARDTPVLALQVLGVPSAVARGHELALSLKRGFALLA
                     YLAYHPGAVPREHLAALLWPDTEAAQARTRLRRLAYTLEEAAGAALFDADTDALALLP
                     GAVETDALRLARFARRAIAAEALADPALAEARHWLVRARRPLLEGVAFGSGAFDDWLK
                     SASLEHEHLVARLLERVIDALARRGQFADALELVEHLLALDPYREPGYVLLMQLHARQ
                     GDGAGVEAAYARCADVLRAEFGIRPGPRTEAAYLRMTEDLRRLMSHRVERPAVRFAES
                     AAGAIAYTVLGAGGHTLVVSPGFVCHIELALEHPPLRAFVEALAERFRVILFDRRGIG
                     LSERLGATSTPAALAQDVQAILDDARVPAAWLFGSSEGGLGAMRLAIDRPERVSGLCL
                     FGSLARGAAAPDYPWALPAAAYDAWLRRLVAGWGGPVGLDTFAPSGQDDPALRAWWAR
                     LVRHAASPGGLETLLRGLRDADLRAELGHIRVPTLVMHRQGDRAVRFDAGRHLAGAIP
                     GAAWRPLEGIDHFWWCGDSDAVLRAILEFTAAT"
     gene            3651..4427
                     /locus_tag="VIN75_12780"
     CDS             3651..4427
                     /locus_tag="VIN75_12780"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8975088.1"
                     /translation="MRALLSILYGLAAYLAFSATLLWFIGFCGNLLVPKSVDIGGAGA
                     AWPAALATDALLLLLFGLQHSVMARRSFKAWWTSIVPPIVERSTYVVASSLVLALVMT
                     CWTPIATPVAWHVDGPAAALLWTGFAIGWAVVALSTFLLDHFELFGLRQAFSALARRP
                     APEVEFRTPLFYRHVRHPLYLGFLVAFWSVPLMTAGHLVFSLGMSAYILVGIAFEERD
                     LVAQFGERYRAYRRQVGMLVPRIGGAGRRSGQGTSGNGLG"
     gene            4546..5049
                     /locus_tag="VIN75_12785"
     CDS             4546..5049
                     /locus_tag="VIN75_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975089.1"
                     /translation="MRLRFLLLPLLLAACSHKPPATDWTPAHWGMMPSEVMNSVPDSA
                     PESGGKLVTGATARVRLESAAVAGATLPAEFYFLDDRLVQVMFSDSQYHDNADSRKTL
                     EHLAAQLRRQYGQETLGKAMDPAAGLSYDASWTAGDTEIILSASPVSASTSMVTVIYR
                     RKGSMSA"
     gene            5081..>5496
                     /locus_tag="VIN75_12790"
     CDS             5081..>5496
                     /locus_tag="VIN75_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8975090.1"
                     /translation="MSPTSPRPEPFAPALRDGVAACRVGVGDGRFPHVLAFLEARFPA
                     VADWPGRLARGDVLDARGRPLPADARVATGDLLWYWRDPPPEPRVPFELELLHRDEHL
                     VVVDKPHFLPAIPAGRHLRETALVRLRAALGIATLAP"
BASE COUNT          702 a         2028 c         2015 g          751 t
ORIGIN      
        1 cgcgtgcggc taacccgccc tacgccgcgc ggcggcgatc gccgcgtcga tcgcctcgag
       61 caccgggtcg acctcgaacg gcttggggat cgcccggaag acgccttcga cgccggtggc
      121 ggcgccggcc gcgcccgcga cgttgccgct cagcgcgatg atcgggatcg ccgccgtcga
      181 cgggtcggcg cgcagcagct cgcgcgcctc gcgccccgtc atctcgggca tgttgttgtc
      241 catcacgatc acggtgggca ggtggcgccg cgcgagctcg acgccgacgc ggccgttggt
      301 ggcggtgagc acctgcacgc cggggcgcgt ggcgagaacg ctctcgatca ggcgcaggtt
      361 cgccaggtcg tcatcgacgc acaggacggt gacctcgggc gagatcgcgg cgctgctggt
      421 cgaccgcgac ggcgcggcgg ccgcgagcgc ggggacgctc gccggcagct cgggctccgc
      481 cggcagctcg atccagaacg tgctgcccac gccctggacg ctgtccacgc cgatgcgccc
      541 gcccatcagc tcgacgagcc ccttggtgac caccaggccg atgccgctgc cctgcgtggt
      601 gcccgcttcg cggcccaggc ggttgaacgg ctggtagagc gcgtccacct gctcgggcgt
      661 catgccggcg ccgtggtcga tgaccgacag ccgcacgttg tcgcccaggc gctggcaggc
      721 gatgtcgacg accgcgtgct cggggctgta cttgacggcg ttcgagagca ggttgatcag
      781 cacctgcttc aggcgcgtgc ggtcgctcat cacgcgcatc gaccggtcgg tgaacgtgcg
      841 cagctcgatg ccgtgcggct cggcgatcgg ccggatcagc gcggtgcagc cagcgagcac
      901 ctcgtcgagc tggagcggct cgatcgccag cgtcgtcttg ccggcctcga tggccgccag
      961 gtcgagcagc tcgttgatga ggtccagcag gtgcttgccg gcgtcgacga tgtgctggcc
     1021 gtacgagacc tgctgcgtga cggacagcgc ctgggtggtg ttgatcgtga gcagctgggc
     1081 gaagcccaga atcgcgttca gcggcgtgcg cagctcgtgg ctgacggtcg acaggaagcg
     1141 gctcttggcg atcgaggcct gctgcgcctg cgcggtggcc tgcgcgagct cgcggttgga
     1201 caccagcagt tcggcggtgc gctggcgcac gcgttcctcg agttcctcgg tatgccgggc
     1261 aaggcgcgcg gcgtcgttca cgcgctgcgt cacgtcgatg gggcacacga agcgcgtgtg
     1321 gcgtcccatg aactccatct cgtgcgacgt gatctccacc aggatgatgt cgccgttctt
     1381 gaggcgatgg cgccagacgc cggaactgaa gaccttggcg ggcgtgtcgc gcaccgaatc
     1441 gcgcaccgcg ggcgcgtcct cggcgggacg gatgtccagg atcgtcatcg ccaggaactc
     1501 gtcgcgcgtc cagccgtagc gcgcgcaggc cacctcgttg acgtcgagga agcgcagggt
     1561 ctcgaggtcg tagacccaca tcggcagcgg gctcgactcg aacagctgcc gatagcgcag
     1621 ctcgccgccg ccgacggtcg ggcacacgcg accgtcctcg cgcggctcgg gggccggcgc
     1681 ggggctcggg tcgggggcgc cagggccgtc ttgcatcgct tctccaggat cgtcgtcggg
     1741 tgctgctccc gcagcatacc gccttcggta tcacgacgga cgggtggccg cccccctggc
     1801 gggggatgcg cgcgggttcg gggcggagcc ggccgggcgc ccgcgaccgc catcgacgga
     1861 ccgcatcgcg gacgttgtgc acgtgcgacg gcggcgctgg tggcgcggac ggcttgccga
     1921 cggcggatca cgtcgccgcc gtgaactcga ggatcgcgcg caggaccgca tcgctgtcgc
     1981 cgcaccacca gaagtggtcg atgccctcga gcgggcgcca ggccgcgccc gggatcgcgc
     2041 cggccaggtg gcgcccggcg tcgaagcgca cggcgcggtc gccctggcgg tgcatcacga
     2101 gcgtgggcac gcggatgtgc ccgagttcgg cgcgcaggtc ggcgtcgcgc aggccgcgca
     2161 ggagggtctc gaggccgccg ggcgaggcgg catggcgcac gaggcgcgcc caccaggcgc
     2221 gcagcgcggg gtcgtcctgg ccgctgggcg cgaaggtgtc caggccgacc gggccgcccc
     2281 agccggcgac caggcgcctg agccacgcgt cgtaggcggc ggccggcagc gcccacgggt
     2341 agtcgggggc cgccgcgccg cgggcgagcg agccgaacag gcacagcccg ctgacgcgct
     2401 cgggccggtc gatggccagt cgcatcgcgc ccagcccgcc ctcggacgac ccgaacagcc
     2461 aggcggccgg cacccgcgcg tcgtcgagga tcgcctgcac gtcctgcgcc agcgcggcgg
     2521 gcgtgctcgt cgcgcccagg cgctcggaca gcccgatgcc gcgccggtcg aacaggatga
     2581 cgcggaatcg ttcggccagc gcctcgacga acgcgcgcag cggcggatgc tccagcgcca
     2641 gctcgatgtg gcacacgaag ccggggctga cgaccagcgt gtgcccgccc gcgccgagca
     2701 ccgtgtacgc gatggcgccg gcggcgctct ccgcgaagcg cacggccggc cgctcgacgc
     2761 gatgcgacat cagccgccgc aggtcctcgg tcatgcgcag gtacgcggcc tccgtgcgcg
     2821 gcccggggcg gatgccgaac tcggcgcgca gcacgtccgc gcagcgcgcg tacgcggcct
     2881 cgacgcccgc gccgtctccc tgccgggcat gcagttgcat cagcagcaca tagccgggtt
     2941 cgcggtacgg gtccagcgcc agcaggtgct cgaccagctc cagcgcgtcg gcgaattgcc
     3001 cgcggcgcgc cagcgcgtcg atcacgcgtt cgagcaggcg cgcgacgagg tgctcgtgct
     3061 cgagcgacgc cgacttcagc cagtcgtcga acgccccgga gccgaacgcg acgccttcga
     3121 gcagcggccg gcgcgcgcgc accagccagt ggcgcgcctc ggccagcgcg gggtcggcca
     3181 gcgcctcggc cgcgatggcg cggcgcgcaa agcgggccag gcgcagcgcg tcggtctcca
     3241 ccgcgcccgg cagcagcgcc agcgcgtcgg tgtcggcgtc gaacagcgca gcgccggccg
     3301 cctcctcgag cgtgtacgcg agccggcgca ggcgcgtgcg ggcctgtgcg gcctcggtgt
     3361 ccggccacag cagcgcggcc aggtgctcgc gcggcacggc cccggggtgg tacgcgagat
     3421 atgccagcag cgcgaagccg cgcttgagcg acagcgccag ctcgtggccg cgcgccacgg
     3481 ccgaagggac gcccagcacc tgcagcgcca gcacgggcgt gtcgcgggcg tcgggtctcg
     3541 atcgggcggc catgggggtc tttatagtcg ggcgaggcgc cgctgcccag ggacgcccaa
     3601 gggacgcccc gcgcccacga tggcaccacc cgctgctcaa ggagtccacc atgcgcgcac
     3661 tgctgtccat cctctacggc ctggccgcct acctggcgtt ctcggccacc ctgctctggt
     3721 tcatcggctt ttgcggcaac ctgctggtgc ccaagtcggt cgacatcggc ggcgccggcg
     3781 ccgcctggcc cgcggcgctc gccaccgacg ccctgctgct gctgctgttc ggcctgcagc
     3841 acagcgtgat ggcgcgccgc agcttcaagg catggtggac gagcatcgtc ccgcccatcg
     3901 tcgagcgcag cacctacgtc gtcgcgagca gcctggtact ggccctggtg atgacatgct
     3961 ggacgccgat cgccacgccc gtggcctggc acgtcgacgg gccggccgcc gcgctgctgt
     4021 ggacgggctt tgccatcggc tgggccgtcg tcgcgctcag caccttcctg ctggaccact
     4081 tcgagctctt cggcctgcgc caggccttct cggccctggc caggcgcccc gcgccggagg
     4141 tggagttccg cacgccgctg ttctaccgcc acgtgcgcca cccgctgtac ctcgggttcc
     4201 tggtcgcgtt ctggtcggtg cccctgatga cggcgggcca ccttgtcttc tcgctcggga
     4261 tgagcgccta catcctggtg ggcatcgcgt tcgaggaacg cgacctggtg gcgcagttcg
     4321 gcgagcgcta ccgtgcgtat cgccggcagg tgggcatgct ggtcccgcgc atcggcggcg
     4381 cgggccgcag gtccggccag ggcacgtccg gcaacggcct gggctgaagc gcccgggcgg
     4441 accgtgaact acttgcggtc cgcggccggg agaaccccag ggcgtatctc ccgcgcggcc
     4501 cggcgttgaa acaatggcgg acaccgatcc gaccgccgcc gtgccatgcg cctccgcttc
     4561 ctgctgctgc cccttctcct cgccgcctgc tcgcacaagc cgccggccac cgactggacg
     4621 cccgctcact ggggaatgat gccctcggag gtgatgaaca gcgtgcccga cagcgcaccg
     4681 gagagcggcg gcaagctcgt caccggcgcc accgcgcggg tgcgtctcga atcggccgcc
     4741 gtggccggcg cgacgctgcc ggccgagttc tacttcctcg acgaccgact cgtgcaggtc
     4801 atgttcagcg actcgcagta ccacgacaac gcggacagcc gcaagacgct ggaacacctc
     4861 gcggcgcagc tgcgcaggca gtacggccag gagacgctcg gcaaggccat ggatcccgcg
     4921 gcgggcctgt cgtacgacgc gagctggacc gccggcgaca cggagatcat cctgtcggcg
     4981 agcccggtgt cggcctcgac gtcgatggtg acggtgatct accggcgcaa gggcagcatg
     5041 agcgcctgac gcgcgtcgcg ccgctgcgaa aatgcggcgc gtgagcccta cgtccccgcg
     5101 ccccgaaccc ttcgcgcccg cgctgcgcga cggcgtcgcc gcctgccgcg tcggcgtcgg
     5161 cgacgggcgc ttcccgcacg tgctggcctt cctcgaggcg cgctttccgg cggtcgccga
     5221 ctggcccgga cggctcgcgc gcggcgacgt gctcgacgcg cgcggccgcc cgctcccggc
     5281 cgacgcgcgc gtcgccacgg gcgacctgct ctggtactgg cgcgacccgc cgcccgagcc
     5341 ccgcgtgccg ttcgagctcg agctgctgca tcgcgacgag cacctggtcg tcgtcgacaa
     5401 gccgcacttc ctgcccgcga tcccggccgg ccgccacctg cgcgagacgg cgctcgtgcg
     5461 cctgcgcgcc gcgctcggca tcgcgacgct ggcgcc
//
LOCUS       DATCRO010000470         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1017296, whole genome shotgun sequence.
ACCESSION   DATCRO010000470 DATCRO010000000
VERSION     DATCRO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017296"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIN75_12795"
     CDS             complement(<1..357)
                     /locus_tag="VIN75_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY8975091.1"
                     /translation="MWFSKLLPREGNFFELFDQHAAHTTEGARAFMSMIQNYNDVALR
                     EKYASEVDVAERAADKVTAEVHRLLHRTFITPIDGEQIRSLINSMDDIADLLQDTSEV
                     LSLYNVTRVSEDVLRLC"
     gene            complement(625..1164)
                     /gene="folK"
                     /locus_tag="VIN75_12800"
     CDS             complement(625..1164)
                     /gene="folK"
                     /locus_tag="VIN75_12800"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8975092.1"
                     /translation="MTTATPPSESSGAWAYVGLGANLGDARATLREALDALRALALPG
                     SFAASSMWGSAPVDSHGPDYVNAVARLRTALGPHALLDALQAIEQRFGRERPYRNAPR
                     TLDLDVLLYGVHGDDGGRVLADDRLVVPHPRAAQRAFVLAPLAELWPHGVIPGAGRVD
                     ALLLAASADPSQRVAVLEA"
     gene            complement(1139..3019)
                     /gene="pcnB"
                     /locus_tag="VIN75_12805"
     CDS             complement(1139..3019)
                     /gene="pcnB"
                     /locus_tag="VIN75_12805"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY8975093.1"
                     /translation="MIRKFIDRLLGKTPAADVLPAGVTLGERAEYGVDQHKIDPKLVA
                     DPAVRVVRTLQDAGYEAYIVGGAVRDLLVGLRPKDFDVATNATPEQVKGLFRRAFIVG
                     RRFRLVHVIYGRGREHEQIEVSTFRAYLDNAVDSAAVQGNEKTSRSELDGKSHAVDAS
                     GRVLRDNVWGPQIEDAARRDFTVNAMYYDPSTQTVVDYHGGIADSKAKLLRMIGDPET
                     RYREDPVRIIRVLRFAAKMGYRIEPKTEAPIRKMKALLANVPASRLFDEMIKLLQTGH
                     SLASLEVLKKYGLDRDTFPILDAHFLGISTDPRREAFVNRALADTDRRVNEGKPVAPS
                     FLMACIFWHEVSQRWQGLRDQGEGLMPALQQAVDATFDARIGDISGRGKLAADMREIW
                     LMQPRFDKRTGSSPAAMLAQPRFRAAFDFMRLRADAGEVDEALAQWWEDYSSGDDHQR
                     RDLVEAARKQQQSKPREPRAAPRDPRPVKAAAPAPAPASAPAPEAQRAPAPAPVPRVS
                     VAKQQMLQLVASARTALSEGATDVERDALRAVIAHPDLPLGSREAEAAPPTATQQLVR
                     DAVDQLKLVLEGQGDAAAARSLLDKVEKKASVVRRRRGGRKKAAGPAFQDDEDDDGDA
                     AE"
     gene            complement(3016..3693)
                     /locus_tag="VIN75_12810"
     CDS             complement(3016..3693)
                     /locus_tag="VIN75_12810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8975094.1"
                     /translation="MTARNLTLFDLDGTLIPGDSDHSFGEFMVAVGWADGDAWRRRND
                     EFYATYVAGALDVHAYVEFATSVWRRRTAQELADIHARFMREVIQPMIHPQALALVRQ
                     HQDAGDLVAIVTATNEFVTRPIAQAFGVPDLLAIELERDAAGAVTGAIRGVPTYQHGK
                     VTRVGQWLQAQGAAWDDFGRVTVYSDSINDLPLMERATDPVATNPSAALEAVATERGW
                     RFLRLFE"
BASE COUNT          520 a         1347 c         1472 g          608 t
ORIGIN      
        1 gcacaggcgc agcacgtcct cgctgacgcg cgtcacgttg tacagcgaca gcacctcgct
       61 ggtgtcctgc agcaggtcgg cgatgtcgtc catcgagttg atcaggctgc ggatctgctc
      121 gccgtcgatc ggcgtgatga acgtgcgatg caaaaggcgg tgcacctcgg ccgtcacctt
      181 gtcggcggcg cgctcggcca cgtccacttc cgaagcgtac ttctcgcgca gcgccacgtc
      241 gttgtagttc tggatcatcg acatgaaggc gcgcgcgccc tcggtcgtgt gcgctgcatg
      301 ctggtcgaac agctcgaaaa agttgccttc gcgcggcagc agcttgctga accacatggt
      361 ctggatcctc ttgggtgcat gacggggccc ggggtcaggc ccgcttttca ggaatcgggg
      421 ggaacgtcga cccggtgcct gccgcttgag gcggcggcgc gcggacgccg gcagggtcga
      481 aatcgtcggt gcggcgcatt gtacgaggcc gccgtcacat ttctgacaca acctcgtacc
      541 tcggtcgcac tgaaaacccg gagggcgcgg catcttgcgc ggccggcgcc cctctcggcg
      601 agggcggcgg gtcagttccg cagttcaggc ctcgaggact gcgactcgtt gcgacggatc
      661 ggccgacgcc gccaacagca gcgcgtccac ccgcccggcg cccgggatca cgccgtgcgg
      721 ccagagctcg gccagcggcg ccaggacgaa ggcgcgctgc gcggcgcgcg gatgcggcac
      781 gaccaggcgg tcgtcggcca gcacgcggcc gccgtcgtcg ccgtgcacgc cgtacagcag
      841 cacgtccagg tcgagcgtgc gcggcgcgtt gcgataaggc cgctcgcggc cgaagcgctg
      901 ctcgatcgcc tgcagcgcgt ccagcagcgc gtgcggcccg agcgcggtgc gcaggcgggc
      961 gacggcattg acgtagtcgg ggccgtgcga gtcgacgggc gcgctgcccc acatcgacga
     1021 ggccgcgaag cttcccggca gggccagtgc gcgcagcgcg tccagggcct cgcgcagggt
     1081 ggcgcgcgcg tcgccgaggt tggcgcccag gccgacgtag gcccaggcgc cggacgactc
     1141 actcggcggc gtcgccgtcg tcatcctcgt cgtcctggaa cgcggggccg gcggccttct
     1201 tgcggccgcc ccggcggcgg cgcacgacgc tggccttctt ctcgaccttg tccagcagcg
     1261 agcgcgcggc ggcggcatcg ccctgccctt cgagcacgag cttgagctgg tcgacggcgt
     1321 cgcgcaccag ctgctgcgtg gcagtgggcg gcgcggcctc ggcctcgcgg ctgccgagcg
     1381 gcaggtccgg gtgcgcgatc accgcccgca gcgcatcgcg ctcgacgtcg gtcgcgccct
     1441 ccgacagcgc cgtgcgggcc gaggccacca gctgcagcat ctgctgcttg gcgacgctca
     1501 cgcgcgggac aggggcgggc gcgggtgcgc gctgcgcttc aggtgcgggg gcgcttgccg
     1561 gcgcgggcgc cggcgcggcg gccttgacgg gacgcgggtc gcgcggcgcg gccctgggct
     1621 cgcgcggctt tgactgctgc tgcttgcgcg cggcctcgac caggtcgcgg cgctggtggt
     1681 cgtcgcccga cgaatagtcc tcccaccact gcgccagcgc ctcgtccacc tcgccggcgt
     1741 cggcgcgcag gcgcatgaag tcgaacgcgg cgcggaagcg cggctgcgcc agcatcgcgg
     1801 ccggcgagct gccggtgcgc ttgtcgaagc gcggctgcat cagccagatc tcgcgcatgt
     1861 cggcggcgag cttgccgcgg ccggagatgt cgccgatgcg cgcgtcgaac gtcgcgtcca
     1921 ccgcctgctg cagcgcgggc atcaggccct cgccctggtc gcgcaggccc tgccagcgct
     1981 gcgacacctc gtgccagaag atgcacgcca tcaggaagct gggcgccacc ggcttgccct
     2041 cgttgacgcg gcggtccgtg tcggccagcg cgcggttgac gaaggcctcg cgcctgggat
     2101 cggtcgagat gcccaggaag tgcgcgtcga ggatggggaa cgtgtcgcga tcgagcccgt
     2161 acttcttgag cacctcgaga ctggccagcg agtggcccgt ctgcagcagc ttgatcatct
     2221 cgtcgaacag gcgcgaggcc ggcacgttgg ccagcagcgc cttcatcttg cggatcggcg
     2281 cttccgtctt cggctcgatc ctgtagccca tcttcgcggc gaagcgcagc acgcggatga
     2341 tgcgcaccgg gtcctcgcgg tagcgcgtct cggggtcgcc gatcatgcgc agcagcttgg
     2401 ccttggagtc ggcgatgccg ccgtggtagt cgaccaccgt ctgggtcgac ggatcgtagt
     2461 acatcgcgtt gacggtgaag tcgcgccgcg cggcgtcctc gatctgcggg ccccagacgt
     2521 tgtcgcgcag cacgcggccg ctggcgtcca cggcgtggct cttgccatcg agctcgctgc
     2581 gcgaggtctt ctcgttgccc tgcacggcgg cggagtcgac ggcgttgtcc aggtaggcgc
     2641 ggaaggtgga gacctcgatc tgctcgtgct cgcggccgcg gccgtagatc acgtgcacga
     2701 ggcggaagcg gcggccgacg atgaaggcgc ggcggaacag gcccttgacc tgctcgggcg
     2761 tcgcgttggt ggcgacgtcg aagtccttgg ggcgcaggcc caccagcagg tcgcgcacgg
     2821 cgccgccgac gatgtaggcc tcgtagcccg cgtcctgcag cgtcctcacg acgcgcacgg
     2881 ccgggtcggc gacgagcttg ggatcgatct tgtgctggtc gacaccgtat tcggcgcgct
     2941 cgccgagcgt gacaccggcg ggcaggacgt cggcggccgg cgtcttgccc agcagtcggt
     3001 cgatgaattt gcggatcatt cgaaaagcct caggaatcgc cagccgcgct cggtggccac
     3061 ggcctccagc gcggccgacg ggttggtcgc gacggggtcg gtggcccgct ccatgagggg
     3121 caggtcgttg atggaatcac tgtaaacggt gacgcggccg aagtcgtccc aggcggcgcc
     3181 ctgcgcctgg agccactggc ccacgcgcgt gaccttgccg tgctggtacg tcggcacgcc
     3241 gcggatggcg ccggtgacgg cgcccgcggc gtcgcgctcg agctcgatgg ccaggagatc
     3301 gggcacgccg aacgcctggg cgatcgggcg cgtgacgaac tcgttggtgg ccgtgacgat
     3361 ggccacgagg tcgcccgcgt cctggtgctg ccggaccagc gccagcgcct gcgggtggat
     3421 catcggctgg atgacctcgc gcatgaagcg ggcgtggatg tcggccagct cctgcgccgt
     3481 gcgtctgcgc cagacggagg tggcgaactc gacgtaggcg tgcacgtcga gcgcgccggc
     3541 cacgtaggtg gcgtagaact cgtcgttgcg gcgccgccag gcatcgccgt cggcccagcc
     3601 gaccgcgacc atgaattcgc cgaacgaatg gtcggagtcg ccggggatga gcgtgccgtc
     3661 caggtcgaac agcgtgaggt tgcgtgcggt cattcggggg acgcttggct tggcctgttc
     3721 ttcgttgagc atgcgcttga gcagcggcac ggtgacggcc cgcttctcgg ccagcgcgaa
     3781 gccgtcgagg cggtgcagca gctgcatcag gctcttgagg tcgcgcgaga agcgggccag
     3841 gatgtagtcc agcacctcgt cgcccagcac gatgccgcgg cggcgcgcct cctggtgcag
     3901 cgcgtcgcgc gtgccggcct cggccagggg atgcacctgg tgcaccg
//
LOCUS       DATCRO010000471         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_353616, whole genome shotgun sequence.
ACCESSION   DATCRO010000471 DATCRO010000000
VERSION     DATCRO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353616"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1242
                     /locus_tag="VIN75_12815"
     CDS             154..1242
                     /locus_tag="VIN75_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HEY8975095.1"
                     /translation="MKSILPRALALAAIVAAGHACAAITLFSHDGFHGKRVTLVQPAP
                     DFRDFNFNDRASSVIVEDEPWEVCQDVGFRNRCAILVPGQYPDLAAVGLGDLLSSARP
                     ARREPPPPPPQPVMPPPPAGDIVFYEHPGFHGRGLGTSSEIDNFQQYGFNDRASSVIV
                     FEHRWEVCEHADFGGRCMILRPGRYPDLRATGLNDMISSVRRVHHEDNFDESRYAPQP
                     LPAYDWRRRPQEYVEQVPIESVHAVYAAPQQQCWVEQSAAPANARTGGAIVGGLIGGI
                     LGHQIGDNGNRTATTAGGTIAGAVIGSQIARGAEQPQQVTRCTSVPAQAPDHYDISYW
                     YHGVRHHVQTTQAPGPTILVNGDGEPRI"
     gene            complement(1285..2460)
                     /locus_tag="VIN75_12820"
     CDS             complement(1285..2460)
                     /locus_tag="VIN75_12820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8975096.1"
                     /translation="MSMPLPPAAPPRALDPPARGPGADGWLARLVFGDVPERRGPLAL
                     TLLAVLVYALFGLVTGWQVVLGLMAFGDWVAWTAISLSGIFGFYLLIRLGISARLGGE
                     PSLTLAQTAFGTASTVFGYAIDPPLRGAVIAIMMLNLVWGMFVLKQRQAQGLCAYALA
                     LLGTTMVWKSTTQPALFPPHIEALHFAFAAILLTATSVLSIRMGELRMRLAARKVELQ
                     DALGTIRALAQVDELTRLSNRRHVFELLKAEQARGVRSSEPLSVVLIDLDHFKSVNDR
                     HGHAVGDAVLRGFAQALREGLRESDGAGRWGGEEFLLVLPRMTAGDAAALVDRLRGVM
                     TGRLLDPTVPELRVSFSAGISECGGGEVAQDAIARADNALYDAKRAGRGRTVVHGRG"
     gene            2603..>2926
                     /locus_tag="VIN75_12825"
     CDS             2603..>2926
                     /locus_tag="VIN75_12825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8975097.1"
                     /translation="MDPRDNLEEFTDPANYDLEQGPPSLARIEFWCRMAARFGGPVLE
                     LCCGTGLVAIPVAAMGLSVTGVDLAAPMLAHAQAKAEHADVDVHWIEADVLALALPRR
                     FGLAIL"
BASE COUNT          443 a         1108 c         1011 g          364 t
ORIGIN      
        1 gcatgggcgg gcgggccggg gatcgggagt cgtttgttgc cgaaacgtca agttacggtc
       61 ggcgtgtcat gcgccggagc gcctcgtgcg ttggacaggg gaggtccgca cctgcaagtg
      121 cgtcgacaat ccacgatcca aacgaggagt cccatgaaat ccatcctgcc ccgcgcgctc
      181 gccctggccg ccatcgtcgc cgccggccac gcctgcgcgg ccatcacgct gttcagccac
      241 gacggcttcc acggcaagcg cgtcacgctg gtgcagcccg cgcccgactt ccgcgacttc
      301 aacttcaacg accgcgcgtc ctcggtcatc gtcgaggacg aaccgtggga ggtgtgccag
      361 gacgtcgggt tccgcaaccg ctgcgccatc ctcgtgccgg gccagtaccc ggacctcgcg
      421 gccgtgggcc tgggcgacct gctgtcgtcg gcgcgtccgg cgcgccggga accgccgccg
      481 cccccgccgc aacccgtgat gccgccgccg cccgccggtg acatcgtgtt ctacgagcat
      541 ccgggcttcc atggccgcgg cctcggcacg tccagcgaga tcgacaactt ccagcagtac
      601 ggattcaacg accgcgcctc gtcggtcatc gtgttcgagc atcgctggga agtgtgcgag
      661 cacgccgact tcggcggccg ctgcatgatc ctgcgcccgg gccgctatcc cgacctgcgc
      721 gccaccggcc tgaacgacat gatctcgtcg gtgcgccgcg tccaccacga ggacaacttc
      781 gacgagagcc gctacgcgcc gcagccgctg ccggcctacg actggcgccg acgcccgcag
      841 gagtacgtcg agcaggtccc gatcgagtcg gtgcacgccg tctatgcggc gccgcagcag
      901 cagtgctggg tcgagcagtc ggccgcgccc gcgaacgcgc gcacgggcgg cgcgatcgtc
      961 ggcggcctga tcggcggcat cctgggccac cagatcggcg acaacggcaa ccgcacggcc
     1021 accaccgcgg gcggcaccat cgccggcgcg gtgatcggct cgcagatcgc ccgcggcgcc
     1081 gaacagccgc agcaggtcac gcgctgcacc agcgtgcccg cgcaggcgcc cgaccactac
     1141 gacatctcgt actggtacca cggcgtgcgc caccacgtgc agacgacgca ggcgcccggc
     1201 ccgaccatcc tggtcaacgg cgacggcgaa ccgcggatct gaccgccacg cgcgggcggg
     1261 cctgacagcc cgccagccag ctagctagcc gcggccgtgc accaccgtgc ggccgcggcc
     1321 cgcgcgcttg gcgtcgtaga gggcgttgtc ggcgcgcgcg atggcgtcct gcgccacctc
     1381 gccgccgcca cactccgaga tgccggccga gaagctcacg cgcagctcgg gcaccgtcgg
     1441 atcgagcagg cgcccggtca tcacgccgcg cagccggtcg accagcgcgg ccgcatcgcc
     1501 cgcggtcatg cggggcagca ccagcaggaa ctcctcgccg ccccagcgtc cggcgccgtc
     1561 gctctcgcgc aggccctcgc gcagcgcctg cgcgaagccg cgcagcaccg cgtcgcccac
     1621 cgcgtggcca tggcggtcgt tgacggactt gaaatggtcc aggtcgatca gcacgacgct
     1681 caacggctcg gacgaacgca cgccgcgcgc ctgttcggcc ttgagcagct cgaagacgtg
     1741 gcggcggttg gacagccgcg tcagctcgtc gacctgggcc agcgcgcgga tcgtcccgag
     1801 cgcgtcctgc agttccacct tgcgcgccgc cagccgcatg cgcagctcgc ccatgcggat
     1861 cgacagcacc gacgtggccg tgagcaggat cgcggcgaac gcgaaatgaa gcgcctcgat
     1921 gtgcggcggg aacagcgccg gctgcgtcgt cgacttccac accatcgtcg tcccgagcag
     1981 cgccagcgcg tacgcgcaca gcccctgcgc ctgccgctgc ttcagcacga acatgcccca
     2041 caccaggttc agcatcatga tggcgatgac ggccccgcgc agcggcgggt cgatggcgta
     2101 gccgaacacg gtggacgcgg tgccgaacgc ggtctgcgcc agcgtcaacg agggctcgcc
     2161 gcccaggcgc gcgctgatgc ccaggcggat cagcaggtag aagccgaaga tgccgctcag
     2221 gctgatcgcc gtccacgcga cccagtcgcc gaacgccatc agcccgagga ccacctgcca
     2281 gccggtcacc agcccgaaca gcgcatagac cagcacggcc agcagcgtca gcgcgagcgg
     2341 gccgcggcgc tcggggacgt cgccgaacac gaggcgggcg agccagccgt cggcacccgg
     2401 cccgcgcgcc gggggatcca gggcgcgagg cggggcggcg ggtggcaggg gcatggacat
     2461 ggcggtgccg tcggaaggcc gattatgcgg ttcgccccct tttgcccggc acgaacggcg
     2521 gcgccctgcg gcgcgcaagc gacaatcggc gccgaggcgc ctgcgcgccg gccgccacca
     2581 ccgccccgca cgaaggacgc cgatggaccc gcgcgacaac ctcgaggagt tcaccgaccc
     2641 cgcgaactac gacctcgagc aagggccgcc gtcgctggcg cgcatcgagt tctggtgccg
     2701 catggcagcg cggttcggcg ggcccgtgct ggagctgtgc tgcggcaccg ggctggtggc
     2761 gatccccgtg gcggcgatgg gcctgtcggt gacgggcgtc gacctcgccg cgcccatgct
     2821 cgcgcatgcg caggcgaagg ccgagcacgc ggacgtcgac gtgcactgga tcgaggccga
     2881 cgtgctcgcg ctcgcgctgc cacgccgctt cggcttggcc atcctc
//
LOCUS       DATCRO010000472         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_938990, whole genome shotgun sequence.
ACCESSION   DATCRO010000472 DATCRO010000000
VERSION     DATCRO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938990"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VIN75_12830"
     CDS             complement(<1..126)
                     /locus_tag="VIN75_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8975098.1"
                     /translation="MAAAAHEQDWDVVIVGSGAGGGTLAARLAECGLRVFLLEAGG"
     gene            complement(144..>2028)
                     /locus_tag="VIN75_12835"
     CDS             complement(144..>2028)
                     /locus_tag="VIN75_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975099.1"
                     /translation="YYTDPTYYNDKDLVRPYRVGMSCGFCHVGPSPTHPPADPAAPRW
                     ADLSSSVGAQYMWVDRLFIVNSNKPEGQRNFMYQLAHTFRPGSMDTSLISTDGINNPR
                     TMNAVYDFPSRMGMAARIGDEKLAGGGLDNKQFNQFPGAPDALKGFFTAPDAVKTPHV
                     LKDGADSVGLLGALNRVYLNIGLFSEEWLLHFNAVVGGKPITPITIATAEKNSAYWRA
                     TEAGTYDTALFFLKAAQPDRLKDAPGGADFLKDDAALVSHGKDVFANTCARCHSSKAP
                     PLPASLDLNAKACAGANYLACFKRYWSYTQTDEYKTAMRAVVQAPDFLQGNYLSTDAR
                     IPVTLLRTNACSPLATNAIAGNIWDNFSSQSYKQLPSVGTITITNPLTGEPMPYAMPA
                     GGRGYTRVPSLISAWSTAPYLLNNSVGTFTTDPSVAGRMKMFDASIEQMLWPEKRQHD
                     AVFGDRILGSIDRTTQRSRIFIPAGYLPKALQPLQGLHRFIPSWVDTGGDVTLDKIPK
                     GVPVGLLSNVILPSETDTNLNEGQRLENIGALALELQADLLKAPDDATDDQLMQTFRN
                     LGPRLLKLSKCPDLVVNRGHYFGTTQFNKQDGLSADEKAFGHEPELTDDDRHALIAFL
                     KTF"
BASE COUNT          290 a          619 c          758 g          361 t
ORIGIN      
        1 gccgccggcc tccagcagga acacgcgcag gccgcactcg gccagccgcg cggcgagcgt
       61 gccgccaccg gcgcccgatc cgacgatgac gacgtcccag tcctgctcgt gcgctgcggc
      121 tgccattccg cgattggaaa gcgtcagaac gtcttgagga aggcgatcag cgcgtggcgg
      181 tcgtcgtccg tcagctcggg ttcatgtccg aaggccttct cgtcggcgga gaggccgtcc
      241 tgcttgttga actgcgtggt gccgaagtag tggccgcggt tgacgaccag gtcggggcac
      301 ttgctgagct tgagcaggcg cgggccgagg ttgcggaacg tctgcatcag ctggtcgtcg
      361 gtggcgtcgt ccggtgcctt gagcaggtcg gcctgcagtt cgagcgcgag ggcgccgatg
      421 ttctccaggc gctggccttc gttcaggttg gtgtcggtct cggacggcag gatgacgttg
      481 gacagcagcc ccaccggcac gcccttgggg atcttgtcca gcgtgacgtc gccgccggtg
      541 tccacccacg acgggatgaa gcggtgcagg ccttgcagcg gctgcagcgc cttgggcagg
      601 tagccggcgg ggatgaagat gcggctgcgc tgcgtggtgc ggtcgatgct gcccaggatc
      661 ctgtcgccga aaacggcgtc gtgctgccgc ttctcgggcc acagcatctg ctcgatcgag
      721 gcgtcgaaca tcttcatgcg cccggccacc gacggatcgg tggtgaacgt gccgacgctg
      781 ttgttgagca ggtagggcgc ggtcgaccac gcgctgatca gcgacggcac gcgcgtgtag
      841 ccgcgcccgc ccgcgggcat cgcgtagggc atgggctcgc cggtgagcgg gttggtgatc
      901 gtgatggtgc ccaccgaggg cagctgcttg tagctctgcg acgagaagtt gtcccagatg
      961 ttgcccgcga tcgcgttggt ggccagcggg ctgcaggcgt tggtgcgcag cagcgtcacg
     1021 gggatgcgcg cgtcggtgga caggtagttg ccttgcagga agtcgggcgc ctggacaacg
     1081 gcgcgcatcg cggtcttgta ctcgtcggtc tgcgtgtagg accagtagcg cttgaagcag
     1141 gcgaggtagt tcgcgccggc gcaggccttg gcgttcaggt ccagcgacgc gggcagcggc
     1201 ggcgccttgc tggagtggca acgggcgcag gtgttggcga agacgtcctt gccgtgcgac
     1261 acgagcgccg cgtcgtcctt gaggaagtcg gcgccgcccg gcgcgtcctt cagccggtcg
     1321 ggctgcgccg ccttcaggaa gaacagcgcc gtgtcgtagg tgccggcctc ggtggcgcgc
     1381 cagtacgcgg aattcttctc cgccgtggcg atcgtgatcg gcgtgatggg cttgccgccg
     1441 acgacggcgt tgaagtgcag cagccactcc tcgctgaaca gcccgatgtt caggtagacg
     1501 cggttgagcg cgcccagcag gccgacggaa tcggcgccgt ccttgagcac gtgcggcgtc
     1561 ttgaccgcgt cgggcgccgt gaagaagccc ttgagcgcgt cgggcgcgcc ggggaactgg
     1621 ttgaactgct tgttgtccag gccgccgccg gcgagcttct cgtcgccgat gcgcgcggcc
     1681 atgcccatgc gcgaaggaaa gtcgtagacc gcgttcatcg tgcgcgggtt gttgatgccg
     1741 tcggtggaga tcagcgacgt gtccatgctg cccgggcgga acgtgtgcgc cagctggtac
     1801 atgaagttgc gctggccctc gggcttgttg gaattgacga tgaacagccg gtcgacccac
     1861 atgtactgcg cgcccaccga cgaactgagg tcggcccatc gcggcgccgc cggatcggcc
     1921 ggcgggtgcg tcgggcttgg accgacgtgg cagaaaccgc aggacatgcc cacgcgatac
     1981 ggccgcacga gatccttgtc gttgtagtac gtcgggtcgg tgtagtag
//
LOCUS       DATCRO010000473         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_938992, whole genome shotgun sequence.
ACCESSION   DATCRO010000473 DATCRO010000000
VERSION     DATCRO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938992"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VIN75_12840"
     CDS             complement(<1..363)
                     /locus_tag="VIN75_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975100.1"
                     /translation="MTPQAFESPARRPAPHRLAVAAALACGALLAACGGGGADAAGPT
                     ETPMSQSDATADAANASSTGSDTAATLDTLVDTTTALAPVLATTAGASPTDRAQVESA
                     PSTISGTVACPGGGSATLS"
     gene            complement(479..1759)
                     /locus_tag="VIN75_12845"
     CDS             complement(479..1759)
                     /locus_tag="VIN75_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975101.1"
                     /translation="MATPTLFRRLVVAQTIGAFALLLVFSSVFYVERNRVVARLTAQR
                     WAPALRAAAGLPAGAASAPAPFSLRVSEQRPDDAIPTNAWAARIVQLRTTLRTEGIPV
                     ADVVFTRALPRPLTWVAVPAADGHLRWYGMQDEVVESYVATRLLIALVLGFAAVAVAS
                     WLIARRLSRPLEALRRRIESHDAPGGDALPAAGMPTLEIAAIDSAWTDLAGRLARQES
                     ERAMLLAGVSHDLRGPLSRIRMAAELLPDTADVAARRASIVRNVDVADRLIASFLDYV
                     RAAELPLQSTVDVVAVARHLLQARDEPADVLMLAAPDRLDVPRSHELLLERLIANLVD
                     NALRHGKPPVRVRLGTDGGDAVIDVEDHGPGIAPDARERLTQAFARGDASRGTPGTGL
                     GLAIVQQIARRMGGTLSFESADGRSCVRVRLPLR"
     gene            complement(1770..2465)
                     /locus_tag="VIN75_12850"
     CDS             complement(1770..2465)
                     /locus_tag="VIN75_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975102.1"
                     /translation="MNSKPLILIVDDDAELSGMVVELLRREGWATHAVLTGGDGERDL
                     AALHPELVLLDVMLPDANGYDLCRRWRALYPALGIVMLTARGDPLDRVLGLEIGADDY
                     LPKPFESRELVARVRALLRRAAPGRAESPLMRFDGLVIDLVRREVRVGEQALALTSVE
                     FKLLVALARAPGTALSREALSDAVQAGSYRPQDRTVDVQVARLRRKLQEVQPGGADWI
                     DTVRGEGYVFVPR"
     gene            2455..>2635
                     /locus_tag="VIN75_12855"
     CDS             2455..>2635
                     /locus_tag="VIN75_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975103.1"
                     /translation="MEFIEGACGVVVGRPECRAAPRARDAMFQRCRISSRRVVAAGAG
                     GAFNEGQRLATLIHPN"
BASE COUNT          344 a          959 c         1003 g          329 t
ORIGIN      
        1 gctcagcgtc gcgctgccgc cgccggggca ggcgaccgtg ccgctgatcg tggatggtgc
       61 cgattcgacc tgtgcgcgat ccgtcggcga tgcgccggct gtggtcgcga gcaccggcgc
      121 gagcgcggtg gtcgtgtcga cgagcgtgtc gagcgtcgcg gccgtgtcgc tgccggtgga
      181 cgacgcgttg gcggcgtcgg cggtggcgtc ggactgcgac atcggcgtct cggtcggccc
      241 ggcggcgtcg gcgccgccgc cgccgcacgc ggccagcagc gcgccgcatg cgagtgcggc
      301 cgcgacggcc aggcggtgcg gagcagggcg gcgggccggg gattcgaagg cttgcggggt
      361 catggtctct ctcctggttg aatcggggtg agagcagtct cccgtcgcgg cgccgcgcgc
      421 cgaccgacat tcggtgaatt gttgcgacag gcctgtcatg gccgcgcaac cagacgtttc
      481 agcgcagcgg aagccgcacg cgcacgcagg acctgccgtc cgcgctctcg aacgacagcg
      541 tgccgcccat gcgccgcgcg atctgctgca cgatcgcgag cccgagaccc gtgcccggcg
      601 tgccgcggct cgcgtccccg cgcgcgaacg cctgcgtcag gcgctcgcgc gcgtcgggcg
      661 cgatgccggg gccgtggtcc tcgacgtcga tgacagcgtc accgccgtca gtgcccaggc
      721 gcacccgcac cgggggcttg ccatgccgca gcgcgttgtc gacgaggttg gcgatgagcc
      781 gctccagcag cagctcgtgc gagcggggca cgtccagccg gtcgggagcg gccagcatca
      841 gcacgtcggc cggctcgtcg cgcgcctgca gcaggtggcg cgcgacggcc acgacgtcca
      901 ccgtcgactg cagcggcagc tcggccgcgc gcacgtagtc caggaagctc gcgatgaggc
      961 ggtcggcgac gtcgacgttg cgcacgatgc tggcccggcg cgcggcgacg tccgcggtgt
     1021 cgggcagcag ctcggcggcc atgcggatgc gcgagagcgg gccgcgcagg tcgtgcgaca
     1081 cgcccgccag cagcatcgcc cgctcgcttt cctggcgcgc gaggcggccg gcaagatcgg
     1141 tccacgcgct gtcgatggcg gcgatctcga gcgtcggcat gccggctgcc ggcagggcat
     1201 cgccgccagg ggcgtcatgc gattcgatgc ggcggcgcaa ggcctccagg ggccggctga
     1261 gccggcgcgc gatcaaccag ctcgcgacgg ccaccgccgc gaagcccagc accagcgcga
     1321 tgagcagcct cgtcgcgacg taggactcga ccacctcgtc ctgcatcccg taccagcgca
     1381 ggtggccgtc ggccgccggc acggcgaccc aggtcagcgg gcgcggcaac gcccgcgtga
     1441 acacgacgtc ggcgacgggg atgccctcgg tccgcagcgt ggtgcgcagc tgcacgatgc
     1501 gcgcggccca cgcgttggtc gggatcgcgt cgtcgggacg ctgctcgctg acgcgcagcg
     1561 agaacggcgc cggcgcgctc gccgcgcccg caggcagccc ggccgccgcg cgcagcgcgg
     1621 gcgcccagcg ctgcgccgtc agccgcgcga cgacgcggtt gcgctccacg tagaacacgc
     1681 tgctgaacac cagcagcagc gcgaacgcgc cgatcgtctg cgcgacgacc aggcggcgaa
     1741 agagggtggg cgtggccacg cgggcgtcct cagcgcggca cgaagacgta gccttcgccg
     1801 cgcaccgtgt cgatccagtc ggctccgccc ggctgcacct cctgcagctt gcggcgcagg
     1861 cgtgccacct gcacgtcgac ggtgcgatcc tgcgggcggt agctgccggc ctgcacggcg
     1921 tcgctgagcg cctcgcgcga gagcgcggtg cccggcgcgc gggccagcgc cacgagcagc
     1981 ttgaattcga cgctggtcag ggccagcgcc tgctcgccga cgcgcacctc gcgccgcacg
     2041 aggtcgatga ccaggccgtc gaagcgcatc agcggcgact ccgcgcgccc gggcgccgcg
     2101 cggcgcagca gcgcccgcac gcgggcgacg agctcgcgcg actcgaaggg cttgggcagg
     2161 tagtcgtcgg cgccgatctc caggcccagc acgcgatcga gcgggtcgcc gcgcgccgtc
     2221 agcatcacga tgcccagcgc cggatacagc gcgcgccagc gtcggcacag gtcgtagccg
     2281 ttggcgtcgg gcagcatcac gtccagcagc accagttccg gatgcagggc ggccaggtcg
     2341 cgctcgccgt cgccgccggt cagcacggcg tgcgtcgccc agccctcgcg ccggagcagt
     2401 tcgacgacca tgcccgagag ctcggcgtcg tcgtcgacga tcaggatcag cggcttggag
     2461 ttcatcgagg gcgcatgcgg cgtcgtggtg ggacgaccgg agtgtagggc ggcgccgcgc
     2521 gcgcgggacg cgatgtttca gcgctgtcgc atttcgtcgc ggcgcgtggt cgcggccggc
     2581 gccggcggcg cgttcaatga gggccagcgc ttggcaaccc tcatccaccc gaacc
//
LOCUS       DATCRO010000474         8585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_443766, whole genome shotgun sequence.
ACCESSION   DATCRO010000474 DATCRO010000000
VERSION     DATCRO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443766"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..3311)
                     /locus_tag="VIN75_12860"
     CDS             complement(48..3311)
                     /locus_tag="VIN75_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8975104.1"
                     /translation="MADAPPNKEARHRLRQLLTRHLLAATAGVALAFALMFLGFGHPL
                     DWVARLSLATAFLALCGVAFGLRRDAGTSLQARALPFVIGAAIGLSGVAAAVAGEGLL
                     SPALAVVGVLLCLFTVIAGLRAGVLAAAVGASMLMLLAGAMAFDWLAPAGLSLGGSPL
                     DLVRPLLTHALVLGIALAGGELLGRTLDRTLRSADERERRFLGLLAVAADAYWEMDGN
                     GRLIRFSAKEELRNFVGLRRHGVDAGMGKLLWETRGVMFEAQVLDAFRSELAARLPMR
                     DVPLRWADVAGRVRHFTLSAEPRTNEAGKFAGYWGVLRDITQDVKARQALWSTETRYQ
                     DLFRRIPTPLVLHREGRLLDANPAGVTLFGFADLRALLGQDLVPFFEEGPSRDRAAEH
                     MQRLDVLAAGEGLPEAEFTLMPGPDRRLTVRVSSVRVDVDGEAATLSIITDDTERRAA
                     EDLVRRSETLLSHLVSTSPDWITLTEFASGRYAMVNPTFLATTGYRAEEVIGRTPSEL
                     GLWADATQRDRIYRLLDENEAVQNFPATMTTRDRRRVSMLVSAARFTIDDVDYQMTSA
                     RDVTASEQARREREAILENALVGISFTRDGRIAMTNARFDEMFGWPRGALLNQLQRVL
                     WPSDASYDTMNLDVGPALSRGEQVDIEMPMMRRDGSTFTGRLLAKALDPAQPASGTIW
                     LAEDVTERRGVEKALARARDEAEAANRAKSAFLANTSHEIRTPLNALVGLAQLARTPE
                     VDDRRRLQYIEQICDSADTLSGILSDILDLSKIEAGKLIVDRVAFDLRGLIGTLKQAY
                     GALADTKGLTLYVNVDEEVPQHVLGDPMRLRQVLTNYLTNALKFTTTGSILLNVQRGT
                     GYRLRFEVIDTGAGMDGPGLARLFQPFSQVDNSLTRKVGGTGLGLSICSELARLMDGT
                     VGVSSEPGKGSVFWVDLPLPPVTAQQLEDHESTSGLDALQGTRVLMVEDNPVNMMIAV
                     ALLERWGAIVDQASDGPGALQAVDHAHQSGDPFDIVLMDVQMPGMSGHEVTRRLRQRY
                     DRHVLPIVALTAAALVSEREDAMAAGMNDFLTKPIDADRLRQTLCNVLVEAEARAER"
     gene            complement(3673..4497)
                     /gene="pyrF"
                     /locus_tag="VIN75_12865"
     CDS             complement(3673..4497)
                     /gene="pyrF"
                     /locus_tag="VIN75_12865"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8975105.1"
                     /translation="MRFTDTLQAASLRNDSALCVGLDPEPAKFPGAWKGDASKVFDFC
                     ARIVDATKDVVSAYKPQIAYFAAQRAEDQLEELMAYIRRVAPGVPVILDAKRGDIGAT
                     AEQYAREAFVRYQADAVTLSPFMGLDSIEPYMKHDGKGLILLCRTSNPGGSDLQAQVL
                     ASGDKLFEHIARLAAGEWNRNGQLALVVGATFPAELARVRELAPTLPLLIPGIGAQGG
                     DVDATVKAGWRADGPIIVSSSRAILYAGSGDDFATAARQVALATRAALNTAIASVR"
     gene            complement(4566..5939)
                     /gene="purB"
                     /locus_tag="VIN75_12870"
     CDS             complement(4566..5939)
                     /gene="purB"
                     /locus_tag="VIN75_12870"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8975106.1"
                     /translation="MPHSALTALSPLDGRYAPKLAALRPLLSEYGLMHRRVQVEVEWL
                     IALSEAGFSEFPVLPESAKGLLRSLVVRFSESDAQAIKDIEKETNHDVKAVEYWLRQR
                     IGHVPELADKLEFIHFACTSEDINNTSHALMLKAAREQVVLPAVDGILTRMQAMAQRF
                     AAVPMLSRTHGQTASPTTVGKEVANVLARLTHARARIADVVLLAKMNGAVGNYNAHVS
                     AYPTTDWEEFARKVVEERLGLKQNAYTIQIEPHDYMAELFDAVVRLNTILVDWSRDVW
                     GYVSLGYFKQKTRPGEVGSSTMPHKVNPIDFENAEGNLGMANALLTHMSQKLPVSRWQ
                     RDLTDSTVLRNMGVAIGYSVLAYDSLTRGLDKLELNEARLAEDLDSAWEVLAEPIQTV
                     MRRHGLPDPYDQLKKFTRGQPMTRELIQGFVAALDLPQEDKARLLAMTPGSYTGIGAQ
                     LAAKIKL"
     gene            6250..7575
                     /locus_tag="VIN75_12875"
     CDS             6250..7575
                     /locus_tag="VIN75_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8975107.1"
                     /translation="MPAAPLSADARDYCAIDFGTSNSAVAVPVAAGMELVPLEPQHGE
                     RGRTMPTAVFYIAEGKDLHALPKLYGRAAIAAYVDGTDGRLMRSMKSVLGSALMEQHT
                     DIGAGRSVAFGDVIASYLLHLRRAASGHAGREIERVVLGRPVFFVDDDSARDAAAQAS
                     LEAAARTVGFKDVRFQYEPIAAAFHYEEQVTREQTVLVADIGGGTSDFSVVRVGPARR
                     GRPDRRDDILASHGVHIAGTDFDRRVELDRILPALGYGAYGPRRGDRAPLEVPSRIYF
                     DLATWHLINTCYRPQRVMEVAAMRDDYANPRHHARLMTTLRDRLGHALAGLAEDAKIA
                     VSQGGTHEVDLFEIERELVVDVTEGQAVAAIEGDLDRIVQAALDTARLAGVPPGAIDA
                     LYFTGGSTGLRLLAERLAAAFPGAQAVHGDRFASVATGLGLEASRHFGA"
     gene            7815..8432
                     /locus_tag="VIN75_12880"
     CDS             7815..8432
                     /locus_tag="VIN75_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8975108.1"
                     /translation="MKLIGSLTSPYVRKVRIVLAEKKLDYNFVLEDVWGSDAILASNP
                     LGKVPVLVMEGAEAVFDSRVIVEYVDTLSPVGKLIPASGRERVEVRTWEALADGILDA
                     CVAARMEAVWTHRTDAQRCQAWIDRQQVRIAAALKSVSQGLGDKAYCVGTHFSLADIA
                     VGCALGYLDFRFPENTWRQDYPNIARLADKLAARQSFIDTAPRTA"
BASE COUNT         1237 a         3056 c         2994 g         1298 t
ORIGIN      
        1 gcgattcgag gagaggccag acccctccga ccacgcctcg ccgccgctca ccgctccgcg
       61 cgcgcctccg cctccaccag cacgttgcac agcgtctgcc gcaggcgatc ggcgtcgatg
      121 ggcttggtaa ggaagtcgtt catgccggcg gccatcgcgt cctcgcgctc ggacacgagc
      181 gcggcggccg tgagcgccac gatgggcagc acgtgccggt cgtaacgctg gcgcagccgg
      241 cgcgtgacct cgtggccgct catgccgggc atctgcacgt ccatcagcac gatgtcgaac
      301 gggtcgccgg actggtgcgc gtggtcgacc gcctgcaacg cgcccggacc gtcgctggcc
      361 tggtcgacga tcgcgcccca gcgctccagc aacgccacgg cgatcatcat gttgaccggg
      421 ttgtcctcca ccatcagcac gcgcgtgccc tgcagcgcgt ccaggcccga ggtgctctcg
      481 tggtcctcga gctgctgcgc cgtgacgggc ggcagcggca ggtcgaccca gaacacgctg
      541 ccctttcccg gctcgctgct gacacccacg gtgccgtcca tcagccgcgc gagctcgctg
      601 cagatcgaca gccccaggcc ggtgccgccg accttgcggg tgagcgagtt gtcgacctgg
      661 ctgaacggct ggaacaggcg cgccagcccg gggccgtcca tgccggcgcc ggtgtcgatc
      721 acctcgaagc gcaggcgata gccggtgccg cgctgcacgt tcagcaggat gctgccggtg
      781 gtggtgaact tcagtgcgtt ggtcaggtag ttggtgagca cctggcgcaa ccgcatcggg
      841 tcgcccagca cgtgctgcgg cacttcctcg tcgacgttga cgtacagcgt caggcccttg
      901 gtgtccgcca gcgcgccgta ggcctgcttg agcgtgccga tgaggccgcg caggtcgaac
      961 gccacgcggt cgacgatcag cttgccggcc tcgatcttgg acaggtccag gatgtccgac
     1021 aggatgcccg acagggtatc ggccgaatcg caaatctgct cgatgtactg cagccgccgc
     1081 cggtcgtcga cctcgggcgt gcgcgcgagc tgcgccaggc cgacgagcgc gttcaggggc
     1141 gtgcggatct cgtggctcgt gttggccagg aacgcgctct tggcgcggtt ggcggcctcg
     1201 gcctcgtcgc gtgcgcgggc cagcgccttc tcgacgccgc gccgctcggt gacgtcctcg
     1261 gccagccaga tcgtgccgct ggccggttgg gccggatcca gcgccttggc cagcaggcgg
     1321 cccgtgaacg tgctgccgtc gcggcgcatc atgggcatct cgatgtcgac ctgctcgccg
     1381 cgcgacagcg ccgggccgac gtcgaggttc atcgtgtcgt acgaggcatc ggacggccac
     1441 agcacccgct gcagctggtt gagcagcgcg ccgcgcggcc acccgaacat ctcgtcgaag
     1501 cgcgcgttgg tcatcgcgat gcggccgtcg cgcgtgaagg agatgcccac cagcgcgttc
     1561 tcgaggatcg cctcgcgctc gcggcgcgcc tgctcgctgg cggtgacgtc gcgcgcgctg
     1621 gtcatctggt agtcgacatc gtcgatcgtg aagcgcgcgg ccgacaccag catcgacacg
     1681 cgccgccggt cgcgcgtggt catcgtggcg ggaaagttct gcacggcctc gttctcgtcg
     1741 agcaggcggt agatgcggtc gcgctgcgtc gcgtcggccc acagcccgag ttcgctgggc
     1801 gtgcggccga tcacctcctc ggcgcggtag ccggtggtcg cgaggaaggt cgggttgacc
     1861 atcgcgtagc gtccgctcgc gaactcggtc agcgtgatcc agtcggggct ggtggacacg
     1921 agatgcgaca gcagcgtctc gctgcggcgc acgaggtctt ccgcggcgcg gcgctcggtg
     1981 tcgtcggtga tgatggacag cgtcgcggcc tcgccgtcga cgtccacgcg cacgctgctg
     2041 acgcgcaccg tcaggcgccg gtcggggccg ggcatcagag tgaactcggc ctcgggcagg
     2101 ccctcgccgg cggccagcac gtccaggcgt tgcatgtgct cggccgcgcg gtcgcgcgac
     2161 gggccctcct cgaagaacgg caccaggtcc tggcccagca acgcgcgcag gtcggcgaag
     2221 ccgaacagcg tgacgccggc cgggttggca tcgagcagac gcccctcgcg atgcagcacc
     2281 agcggcgtgg ggatgcggcg gaacaggtcc tggtagcggg tctccgtcga ccacagcgcc
     2341 tggcgcgcct tgacgtcctg cgtgatgtcg cgcagcacgc cccagtagcc cgcgaacttg
     2401 ccggcctcgt tcgtgcgcgg ctcggcgctg agcgtgaagt ggcggacgcg gcccgcgacg
     2461 tcggcccagc gcagcggcac gtcgcgcatg ggcaggcgcg ccgcgagctc cgagcggaat
     2521 gcgtccagga cctgcgcctc gaacatcacg ccgcgcgtct cccacagcag cttgcccatg
     2581 ccggcgtcga ccccgtggcg gcgcaggccg acgaagttgc gcagctcctc ctttgcgctg
     2641 aagcggatga ggcggccgtt gccgtccatc tcccagtacg catcggcggc cacggccagc
     2701 aggcccagga agcggcgctc gcgctcgtcg gcactgcgca gcgtgcggtc cagcgtgcgc
     2761 ccgagcagct cgccgccggc cagcgcgatg cccagcacca gcgcgtgggt cagcagcggg
     2821 cgcacgaggt cgagcggcga gccgcccagc gagaggccgg ccggcgccag ccagtcgaag
     2881 gccatcgcgc cggccagcag catcagcatg ctcgccccga ccgccgcggc cagcacgccc
     2941 gcgcgcaggc cggcgatgac ggtgaacagg cacagcagca cgcccaccac ggccagtgcc
     3001 ggcgacagca ggccctcgcc ggcgaccgcc gcggccacgc ccgacagccc gatcgccgcg
     3061 ccgatcacga acggcagcgc gcgcgcctgc aggctggtgc ccgcgtcgcg ccgcagcccg
     3121 aaagcgacgc cgcacagcgc gaggaacgcc gtcgccagcg acagccgggc cacccagtcc
     3181 agcggatggc cgaacccgag gaacatcagc gcgaacgcga gcgcgacccc tgccgtcgcg
     3241 gccagcaggt ggcgcgtcag caactggcgc aggcggtgcc tggcctcctt gttcggcggt
     3301 gcatcagcca tcgggtgctc tttttattgg cccttggcga cccgatcacg gctcgccccc
     3361 caaggcccct agtttgactc gaattctcga gttgcaccgt gacgaggctg aacttccgat
     3421 gcagaacgtg cgttcagctg atgagttgag agaaaaggtc tgcccctttc cacgcgagcg
     3481 cgtgcgaggg tgtcaggtct tgaacgttgc gccttgagcg tttcgccgag ggtccgccga
     3541 ggggtcaggt cttgaaagat gcacgacctg ctgtcgtcct gcagcgaaga tgtcgccgtg
     3601 caactttcaa gacctgaccc caacgccagg accttgccgc gcgactttca agacctgaca
     3661 ccggctatcg cttcagcgca cggaagcgat cgccgtattc agcgccgcgc gcgtggccag
     3721 cgccacctgg cgggccgcgg tggcgaagtc gtcgccgctc cccgcgtaca ggatcgcgcg
     3781 cgaggagctg acgatgatcg ggccgtcggc gcgccagccg gccttgacgg tggcgtcgac
     3841 gtcgccgccc tgcgcgccga tgccggggat cagcagcggc agcgtgggcg cgagctcgcg
     3901 cacgcgggcc agctcggccg ggaacgtggc gcccacgacg agcgccagct ggccgttgcg
     3961 attccactcg cccgcggcga ggcgggcgat gtgctcgaac agcttgtcgc cgctcgccag
     4021 cacctgcgcc tgcaggtcgg agccgccggg gttggaggtg cggcacagca ggatcaggcc
     4081 cttgccgtcg tgcttcatgt agggctcgat cgagtccaag cccatgaacg gcgacagcgt
     4141 caccgcgtcg gcctggtagc gcacgaaggc ctcgcgggcg tactgctcgg cggtggcgcc
     4201 gatgtccccg cgcttggcgt cgaggatgac gggcacgccc ggcgcgacgc ggcggatgta
     4261 ggccatcagc tcttccagct ggtcttccgc gcgctgcgcg gcgaagtagg cgatctgcgg
     4321 cttgtaggcg gagacgacgt ccttggtggc gtcgacgatg cgcgcgcaga agtcgaacac
     4381 cttgctggcg tcgcccttcc acgcgccggg gaacttggcc ggttcgggat cgagccccac
     4441 gcacagcgcg gagtcgttgc ggagcgaggc ggcttgcagg gtgtcggtga atcgcatcgt
     4501 cggtgtcctt cgttggtgtt gtggggcgga tggaaaaacg cggcccggag gccgcgtcgt
     4561 caggatcaga gcttgatttt cgcggccagc tgcgcgccga tgccggtgta gctgcccggc
     4621 gtcatcgcga gcaatcgcgc cttgtcttcc tgcggcaggt ccagcgccgc gacgaagccc
     4681 tggatcagct cgcgcgtcat cggctggccg cgcgtgaact tcttgagctg gtcgtacggg
     4741 tcgggcaggc cgtggcggcg catgaccgtc tggatgggct cggccagcac ttcccacgcg
     4801 gagtcgaggt cttcggccag gcgcgcctcg ttcagctcga gcttgtccag gccgcgggtg
     4861 aggctgtcgt aggccagcac ggagtagccg atggccacgc ccatgttgcg cagcaccgtc
     4921 gaatcggtga gatcgcgctg ccagcggctg acgggcagct tctgcgacat gtgcgtcagc
     4981 agcgcgttgg ccatgcccag gttgccttcg gcgttctcga agtcgatcgg gttgaccttg
     5041 tgcggcatcg tcgacgaacc gacctcgccc ggcctggtct tctgcttgaa gtagcccagg
     5101 ctgacatagc cccagacgtc gcgcgaccag tccacgagga tcgtgttcag gcgcacgacg
     5161 gcgtcgaaca gctcggccat gtagtcgtgc ggctcgatct ggatcgtgta cgcgttctgc
     5221 ttcaggccca ggcgctcctc gacgaccttg cgcgcgaact cctcccaatc ggtggtgggg
     5281 taggccgaca cgtgcgcgtt gtagttgccg accgcgccgt tcatcttggc cagcagcacc
     5341 acgtcggcga tgcgcgcgcg ggcgtgcgtc aggcgcgcga gcacgttggc cacttccttg
     5401 cccaccgtgg tggggctggc cgtctggccg tgcgtgcgcg acagcatcgg caccgccgcg
     5461 aagcgttgcg ccatcgcctg catgcgcgtg aggatgccgt cgaccgccgg cagcaccacc
     5521 tgctcgcgcg cggccttgag catcagcgcg tggctggtgt tgttgatgtc ctcgctggtg
     5581 caggcgaagt ggatgaactc cagcttgtcg gcgagctcgg gcacgtggcc gatgcgctgg
     5641 cgcagccagt actcgacggc cttgacgtcg tggttggtct ccttctcgat gtccttgatc
     5701 gcctgcgcgt cggactcgga gaagcgcacc accagcgagc gcagcaggcc cttggccgat
     5761 tcgggcagga ccgggaactc ggaaaagccg gcttccgaca gcgcgatcag ccactccacc
     5821 tcgacctgca cccggcgatg catcaggccg tactcggaca gcaacgggcg cagcgcggcc
     5881 agcttggggg cgtaacggcc gtcgaggggc gacagggcgg tgagggcgga atggggcatg
     5941 gaggggctcg cgtggacgcg tcgcgtcggg ggatgaggcg ggcagcgggc gtggaggcgg
     6001 ccaccggatc gcaaaacctg ctgattttac gggtcgcggc gctcccgggc taggggcaac
     6061 ccgaagtccg gcccccatta ccgggcggaa aaccgaaagc gaatgccacg gaaggccccg
     6121 ccggcagggc cttccatttc gcggggtcag gtcttgaacg ttgcggggtc aggtcttgaa
     6181 tcgtgcattg acgcctcccg gcgcgcctcg cacaatcacg ccccttttcg ctttccgtcc
     6241 cccgttccca tgccggccgc ccctctttcc gccgacgcgc gcgactactg cgccatcgac
     6301 ttcggcacct ccaactcggc cgtcgccgtc cccgtggccg ccggcatgga gctcgtgcca
     6361 ctcgagccgc agcacggcga acgcgggcgc acgatgccca ccgcggtgtt ctacatcgcc
     6421 gagggcaagg acctgcacgc gctgcccaag ctctacggac gcgccgcgat cgccgcctac
     6481 gtcgacggca ccgacggccg cctgatgcgc tcgatgaaga gcgtgctcgg ctcggcgctg
     6541 atggagcagc acaccgacat cggcgccggc cgctcggtcg ccttcggcga cgtcatcgcc
     6601 agctacctgc tgcacctgcg gcgcgccgcg agcgggcacg ccgggcgcga gatcgagcgc
     6661 gtcgtgctgg gccgcccggt gttcttcgtc gacgacgact ccgcgcgcga cgccgccgcc
     6721 caggcctcgc tggaagccgc ggcgcgcacg gtcggcttca aggacgtgcg cttccagtac
     6781 gagccgatcg cggccgcgtt ccactacgag gaacaggtca cgcgcgagca gaccgtcctc
     6841 gtggccgaca tcggcggcgg cacgtccgac ttctccgtcg tgcgcgtcgg ccctgcgcgc
     6901 cgcggccggc ccgaccgccg cgacgacatc ctcgccagcc acggcgtgca catcgccggc
     6961 accgacttcg accgccgcgt cgagctcgac cgcatcctgc ccgcgctcgg ctacggcgcc
     7021 tacggcccca ggcgcggcga ccgcgcgccg ctggaggtgc ccagccgcat ctacttcgac
     7081 ctcgccacct ggcacctgat caacacctgc taccggccgc agcgcgtgat ggaggtcgcg
     7141 gcgatgcgcg acgactacgc caacccacgc caccacgcgc gcctgatgac gacgctgcgc
     7201 gaccgcctcg gccacgcgct ggccggcctg gccgaggacg cgaagatcgc cgtgtcgcag
     7261 ggcggcacgc acgaggtcga cctgttcgag atcgaacgcg aactggtcgt cgacgtgacc
     7321 gagggccagg cggtcgccgc gatcgagggc gacctcgacc gcatcgtgca ggccgcgctc
     7381 gacaccgcgc gcctggccgg cgtgccaccg ggcgccatcg acgcgctgta cttcaccggc
     7441 ggctccaccg gactgcgcct gctggccgag cgcctggccg ccgcgttccc gggcgcgcag
     7501 gccgtgcacg gcgaccgctt cgccagcgtc gcgaccgggc tcgggctgga ggcgagccgg
     7561 cacttcggcg cctaggcccg ccactgtcgt gttgcgccgg gcacacacgc gcccccctgt
     7621 tcttggccgc atcctggtcc gccacgtgct gcatcatcgc cgcggccgcc gcatggccgc
     7681 ggcttcggca cgttgttggt gcgcgctcgc cacgccgcgc cggcgacgca aaacccccgg
     7741 aagagggcct tgcgtgtgcc gtttttcacg gggtgcttct atagaatgaa gcccagtcct
     7801 gactcgctgc ccacatgaaa ctcatcggat ctctcaccag cccctacgtt cgcaaagtgc
     7861 gcatcgtgct ggcggagaag aagctcgact acaacttcgt gctcgaggac gtctggggca
     7921 gcgacgccat cctggcctcc aatccgctgg gcaaggtgcc cgtgctcgtc atggaaggcg
     7981 ccgaggccgt gttcgactcg cgcgtcatcg tcgaatacgt cgacaccctg tcgcccgtgg
     8041 gcaagctcat cccggcctcc ggccgcgagc gcgtggaagt gcgcacctgg gaggcgctgg
     8101 ccgacggcat cctcgacgcg tgcgtggccg cgcgcatgga ggcggtctgg acgcaccgca
     8161 ccgacgcgca gcgctgccag gcctggatcg atcgccagca ggtgcgcatc gccgcggcgc
     8221 tgaagtccgt cagccagggc ctgggcgaca aggcgtactg cgtcggcacg catttctcgc
     8281 tggccgacat cgccgtcggc tgcgcgctcg gctacctcga cttccgcttt cccgagaaca
     8341 cctggcgcca ggattacccg aacatcgcgc gcctggccga caagctggcc gcgcgccaga
     8401 gcttcatcga caccgcgccg cgcacggcct gagccacgcg acgcacgcga cgaaggcggc
     8461 cgaaggccgc cttttttcat gcctgcgcgg gaacgcgcgc acgcgcggcc gcggtcgcgc
     8521 tgcgagaatc gcctgacaag acgcctggga gggatggcat gaacaacgca cgaacgatcc
     8581 gagcg
//
LOCUS       DATCRO010000475         7469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_792916, whole genome shotgun sequence.
ACCESSION   DATCRO010000475 DATCRO010000000
VERSION     DATCRO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792916"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VIN75_12885"
     CDS             complement(<1..330)
                     /locus_tag="VIN75_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY8975109.1"
                     /translation="MRCPYCSHDETQVAETRESDEGDVVRRRRRCLKCDKRFTTYERA
                     EMAFPSIVKKSGARVDFDPAKLRGSMTLALRKRQVSIEQIDAAIERIQDKLIGTGLRE
                     VPSTRVGE"
     gene            complement(350..1597)
                     /gene="glyA"
                     /locus_tag="VIN75_12890"
     CDS             complement(350..1597)
                     /gene="glyA"
                     /locus_tag="VIN75_12890"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8975110.1"
                     /translation="MFDRQQSTIANVDPAIWAAIQAENLRQEQHIELIASENYTSPAV
                     MAAQGSQLTNKYAEGYPGKRYYGGCENVDVVEQLAIDRIKQLFGAENANVQPNSGSQA
                     NQAVFLGLLQPGDTIMGMSLAEGGHLTHGMALNMSGKWFKVVSYGLNAQEDIDYDAME
                     RTAREHKPKLIIAGASAFALRIDFERFARIAKEIGATFMVDMAHYAGLIAAGVYPNPV
                     PHADVVTSTTHKSLRGPRGGIILMKDAFAKQINSAIFPGLQGGPLMHVIAGKAVAFQE
                     ALQPEFKAYQQQVVRNADVLARTLIERGLRIVSGRTESHVMLVDLRAKGITGKDAERI
                     LGEAHMTCNKNAIPNDPEKPMVTSGIRLGTPAMTTRGFKEAEARATAHLIADVLDAPH
                     DPARLAAVRAKVEALTRDFPVYR"
     gene            complement(2003..2950)
                     /locus_tag="VIN75_12895"
     CDS             complement(2003..2950)
                     /locus_tag="VIN75_12895"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8975111.1"
                     /translation="MLQPQAVVRNALTTGRESVKVFLTDVGHGLLDVSHSMLAMVGLA
                     VVAGALFMSSQDDLRDSLEARAWGWLEARHLARSGGEDLAAAAESEPTSAGPINTSST
                     ADPADLTRAQANVARWIVRRYHVAPEPVARLVQESWTVGQRTGIEPTLILAVMAIESS
                     FNPIAQSPVGAQGLMQVMTKVHSDKYESYGGHLAAFDPVSNLRVGVLVLKECIAKGGG
                     LANGLHYYVGAGALDDDGGYAAKVMAEQELLKRVAAGQNVPPTVVPMVANVAPAPAAT
                     PAPAAAPAAAASESTAHAAAPEQQDAQAKPERERVALAH"
     gene            3198..4775
                     /locus_tag="VIN75_12900"
     CDS             3198..4775
                     /locus_tag="VIN75_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY8975112.1"
                     /translation="MKYRDLRDFAANLEGFGELRRVTAPVSPQLEMTALADRVLRQEG
                     PALWFENPTGYDVPVLANLFGTPHRVALGMGAADISELREIGQLLANLREPEPPKSIK
                     DAGKLWTMGKALWDMKPAVLRSGPCQEVVQDAGEIDLSRWPIQTCWPGDAAPLVTWGL
                     VVTRGPRGVPKPRLRQNLGIYRQQVIGRRELIMRWLAHRGGALDFRDFARVNPGKPFP
                     LVCALGADPATTLGAVTPVPDSLGEYQFAGLLRGGRTELVDTQTGEDGLMLQVPARAE
                     IVLEGHIPVAEPGLAGHSEHGVPLKEINGYLHALEGPYGDHTGYYNERDWFPVFRLDR
                     LTHRRDPVYHSTYTGKPPDEPAVLGVALNEVFVPLLQKQFPEIVDFYLPPEGCSYRMA
                     VISMRKAYAGHAKRLMFGLWSVLRQFMYTKFIVVVDDDIDIRDWNEVIWAITTRMDPV
                     RDTTLVDHTPIDYLDFASPVSGLGGKMGLDATSKWPGETSREWGRPITMDGALTKDVE
                     ARIDALFSQVMAPVKGQ"
     gene            complement(4996..5832)
                     /locus_tag="VIN75_12905"
     CDS             complement(4996..5832)
                     /locus_tag="VIN75_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY8975113.1"
                     /translation="MLADWMTAAGLGALKPALAALVLPPVPFLALAFAGAALARRRPR
                     ASRWLVAVGCACAWISCCSGAARWVEQSFLDEPAPLDAAQRADLKARAAAGQSLAIVV
                     LGGGMEARPAEYASPDLGNASLKRLRYGIWLGRETGIPVAASGGRGWVAADASVPAEA
                     TRMAGIARDEFNAPLRWVEARSRDTHENAVDTLALLQPAGVREIVLVTHGSHMPRALR
                     EFRAAAAARSASSPVAITPASMGQAYPASSPVTRWLPSGAGLERMQLALHEVLARLVD
                     HR"
     gene            complement(5954..6598)
                     /gene="pdxH"
                     /locus_tag="VIN75_12910"
     CDS             complement(5954..6598)
                     /gene="pdxH"
                     /locus_tag="VIN75_12910"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY8975114.1"
                     /translation="MDHSKKIADERKSYERDALEESDAALDPLRQFQAWMDEAFAAKV
                     PEPNAMTLATVGADGRPSTRIVLIKGYDARGIVWFTNYDSRKGRELAVHPLAALQFHW
                     VELERVVRIEGAVERVDDAESDAYFASRPLDSRIGAWASPQSEVIESRAVLVANAARY
                     AAKFLLNPPRPPHWGGFRLKPDTWEFWQGRKSRLHDRLRYRLDGTAWTRERLAP"
     gene            6727..7338
                     /locus_tag="VIN75_12915"
     CDS             6727..7338
                     /locus_tag="VIN75_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8975115.1"
                     /translation="MKNEPAVVVLAAGAGSRYQGARHKLSEKLGLDSVLVRTLRNAIA
                     SEMSVVLVISDALVSEARGLIAPEDMVVVDARSQTGWGMGDSIAAGVSQHASATGWLV
                     LPADMPLVRPGSLRAVADALDQQPIAFAQHRGRRGHPVGFAAELFSELVMLKGDEGAR
                     RLLARYPTAAVELDDPGVLLDIDTVDDLAAAQRRIDGSSEIAI"
BASE COUNT         1061 a         2568 c         2666 g         1174 t
ORIGIN      
        1 ctcgccaacg cgcgtggagg gcacctcgcg caggccggtg ccgatcagct tgtcctggat
       61 gcgctcgatg gccgcgtcga tctgctcgat gctcacctgg cgcttgcgca gcgccagcgt
      121 catcgagccg cgcagcttgg cggggtcgaa gtcgacgcgg gcgccgctct tcttgacgat
      181 cgacgggaac gccatctcgg cgcgctcgta ggtcgtgaag cgcttgtcgc acttcaggca
      241 gcggcggcgc ctgcggacga cgtcgccctc gtccgattcg cgggtctcgg cgacctgcgt
      301 ctcgtcatga ctgcaatagg ggcagcgcat ggggcgacgg cgccgtggct cagcggtaga
      361 ccgggaagtc gcgcgtcagc gcctcgacct tggcgcgcac ggcggccagc cgggccgggt
      421 cgtgcggcgc gtccagcacg tcggcgatca ggtgggccgt ggcgcgcgcc tctgcttcct
      481 tgaagccgcg cgtggtcatg gccggcgtgc ccagacggat gccgctggtg accatcggct
      541 tttccggatc gttggggatc gcgttcttgt tgcaggtcat gtgcgcctcg cccaggatgc
      601 gctcggcgtc cttgccggtg atgcccttgg cgcgcaggtc gaccagcatc acgtggctct
      661 cggtgcggcc cgagacgatg cgcaggccgc gctcgatcag cgtcctggcc agcacgtcgg
      721 cgttcctgac gacctgctgc tggtaggcct tgaactcggg ctgcagcgcc tcctggaacg
      781 caaccgcctt gccggcgatc acgtgcatca gcgggccgcc ttgcaggccc gggaagatgg
      841 ccgagttgat ctgcttggcg aacgcgtcct tcatcaggat gatgccgccg cgcgggccgc
      901 gcaggctctt gtgcgtggtg gatgtgacga cgtcggcgtg cggcaccggg ttcggataga
      961 cgcccgcggc gatcaggccg gcgtagtgcg ccatgtcgac catgaaggtc gcgccgatct
     1021 ccttggcgat gcgcgcgaag cgctcgaagt cgatgcgcag cgcgaacgcc gacgcgccgg
     1081 cgatgatcag cttgggcttg tgctcgcgcg cggtgcgctc catcgcgtcg tagtcgatgt
     1141 cttcctgcgc gttcaggccg taggacacga ccttgaacca cttgccgctc atgttcagcg
     1201 ccatgccgtg cgtgaggtgg ccgccctcgg ccaggctcat gcccatgatg gtgtcgccgg
     1261 gctgcagcag gcccaggaac acggcctggt tggcctgcga gccggagttg ggctgcacgt
     1321 tggcgttctc ggcgccgaac agctgcttga tgcggtcgat ggccagctgc tcgacgacgt
     1381 ccacgttctc gcagccgccg taatagcgct tgccggggta gccttcggcg tacttgttgg
     1441 tcagctgcga gccttgcgcg gccatgacgg ccggcgaggt gtagttctcc gaggcgatca
     1501 gctcgatgtg ctgttcctgg cgcaggttct cggcctggat ggcggcccag atggcggggt
     1561 caacgttggc gatcgtcgat tgttggcggt cgaacatgga ggttccttgg gatttgaggg
     1621 caatcttggc gcgtcggttg ggcctcgcgg acagcggtcg tcgatgcgcg aggccatcga
     1681 cggtcgcaga tccagcggtg cgcgaaggac gcgctgccca ggcgaacggc tgaaagggcg
     1741 gcgcccggac tgaggctccc ggcggccctt cgcgcttccc ggtggtggcc cacctcgcaa
     1801 cgcgccctgg aggccggcgg gtgccgctcc ggggacgcgt cctgtcgcca gtcgcgcgag
     1861 ccgccagttt aaccggctgg cccggggcgc gggtgggcgc gagcgtcgat tcgcgcaata
     1921 cgaagcaggc cgggcgtgcg cgcgccgggc ggccgaatga aaaacgggcg ccgaggcgcc
     1981 cgtcgaacgg ttgtctggcg gctcagtgcg ccagcgccac gcgttcgcgt tcgggcttgg
     2041 cctgcgcgtc ctgctgctcg ggcgcggcgg cgtgggcggt cgactcgctg gcggccgcgg
     2101 ccggcgcggc ggcaggcgcg ggcgtcgccg cgggggcggg cgcgacgttg gcgaccatcg
     2161 gcaccaccgt cggcgggacg ttctggccgg ccgcgacgcg cttgagcagc tcctgctcgg
     2221 ccatcacctt ggccgcgtag ccgccgtcgt cgtcgagcgc cccggcgccg acgtagtagt
     2281 gcaggccgtt ggccaggccg ccgcccttgg cgatgcattc cttgagcacc agcacgccca
     2341 cccgcaggtt gctgacgggg tcgaacgccg ccaggtggcc gccgtaggac tcgtacttgt
     2401 cgctgtgcac cttggtcatc acctgcatca ggccctgcgc gcccaccggg ctttgcgcga
     2461 tcgggttgaa gctggactcg atggccatca cggccaggat cagcgtcggc tcgatgccgg
     2521 tgcgttggcc gaccgtccac gattcctgga ccaggcgtgc cacgggctcc ggcgccacat
     2581 ggtagcggcg gacgatccag cgggccacgt tggcctgggc gcgcgtcagg tcggccggat
     2641 cggcggtcga cgacgtgttg atcgggccgg cggacgtcgg ctcggactcg gccgcggcgg
     2701 ccaggtcctc gccaccgctg cgggcgaggt ggcgggcttc cagccagccc caggcacggg
     2761 cctcgaggct gtcgcgcagg tcgtcctggc tgctcatgaa cagggcgccg gccacgacgg
     2821 ccagtcccac catggccagc atgctgtggc tcacgtcgag caggccgtgg cccacgtcgg
     2881 tcaggaagac cttcaccgat tcgcgcccgg tggtcagtgc gttgcgcacg acggcttggg
     2941 gttgaagcat ctctctcctt tcacagcgcc gggccggctg acggcgctca gcggcgcttc
     3001 gtcgcggcgt ccagggacgc tggcgataat ggttcgctgc gattcatggc cggggtgtct
     3061 gcgtctagca accggcggca gccttcaggg tccgcctcgc tgggcaggga taaccctggt
     3121 gttacgtctg gccggattat gggagccgcg taataacccg tcaacaatat aagcctctca
     3181 agtcataacc aaaacttatg aaataccgtg atttgcgcga tttcgcggcc aatcttgagg
     3241 gttttgggga gctcagacgt gtaacagcgc ccgtgtcccc ccagctcgaa atgaccgcgc
     3301 tggccgaccg ggtcctccgg caggaaggcc ctgcgctgtg gttcgagaac cccaccgggt
     3361 atgacgtgcc cgtcctcgcc aacctgttcg gcacccccca tcgcgtcgcc ctcggaatgg
     3421 gcgcggccga catctccgag ttgcgggaga tcggccagct gctggccaac ctgcgggagc
     3481 cggagccgcc gaagtcgatc aaggacgccg gcaagttgtg gaccatgggc aaggcgctgt
     3541 gggacatgaa gcccgccgtg ctgcgcagcg gaccgtgcca ggaggtcgtc caggacgccg
     3601 gcgagatcga cctgtcgagg tggccgatcc agacctgctg gcccggcgac gccgccccgc
     3661 tggtgacctg gggcctggtc gtcacccgcg gcccgcgcgg cgtgcccaag ccgagattgc
     3721 ggcagaacct gggcatctac cgccaacagg tgatcggccg gcgcgagctc atcatgcgct
     3781 ggctggccca tcgcggcggc gcgctcgact tccgcgactt cgcccgcgtc aacccgggca
     3841 agccgttccc gctggtgtgc gcgctgggcg ccgacccggc cacgacgctg ggcgccgtca
     3901 cgccggtgcc cgacagcctg ggcgagtacc agttcgccgg cctgctgcgc ggcggccgca
     3961 cggagctggt cgacacgcag accggcgagg acggcctgat gctgcaggtg cccgcgcggg
     4021 ccgagatcgt cctggagggc cacatcccgg tggccgagcc gggcctggcc ggccacagcg
     4081 agcacggcgt gccgctcaag gagatcaacg gctacctgca cgcgctcgag ggcccgtacg
     4141 gcgaccacac cggctactac aacgagcgcg actggttccc ggtgttccgc ctcgaccgcc
     4201 tgacgcaccg ccgcgacccc gtctaccact cgacctacac cggcaagccg cccgacgagc
     4261 ccgcggtgct gggcgtggcg ctcaacgagg tcttcgtgcc gctgctgcag aagcagttcc
     4321 ccgagatcgt cgacttctac ctgccgcccg agggctgcag ctaccgcatg gccgtcatct
     4381 cgatgaggaa ggcctacgcg ggccacgcca agcggctgat gttcggcctg tggagcgtgc
     4441 tgcgccagtt catgtacacg aagttcatcg tggtggtcga cgacgacatc gacatccgcg
     4501 actggaacga ggtgatctgg gcgatcacga cgcgcatgga tcccgtgcgc gacaccacgc
     4561 tggtcgacca cacgccgatc gactacctcg acttcgcgtc gccggtcagc ggcctgggcg
     4621 gcaagatggg cctggacgcg acgtcgaagt ggcccggcga gacgagccgc gaatggggcc
     4681 ggccgatcac gatggacggt gcgctcacga aggatgtgga ggcgcgaatc gacgcgcttt
     4741 tctcgcaagt catggcgccc gtcaagggcc aatgaggcta tcaggacaac ctgatcattc
     4801 gcggatattt ccttgcgacg atcggtcgcg aggactatct ttgaatcgcc tcaaggaggc
     4861 actcccctct gcgcagtcga tgcgcaatcg gggccccggg cgttctgctc cggatcccca
     4921 tcgggcgacg ttcaagtcgc ccaggaagcc ccgatcatcc tcaagtgatc ggggcttcgt
     4981 catgtccgga tcggctcagc gatggtcgac caggcgggcc agcacctcgt gcagcgcgag
     5041 ttgcatgcgt tcgagcccgg cgcccgaggg cagccagcgc gtgacggggc tggacgcggg
     5101 gtaggcctgg cccatcgacg cgggcgtgat cgcgaccggg ctcgacgccg accgcgccgc
     5161 cgcggccgcg cggaactcgc gcagcgcacg cggcatgtgc gagccgtgcg tcacgagcac
     5221 gatctcgcgc acgcccgcgg gctgcagcag cgcgagcgtg tcgacggcgt tctcgtgcgt
     5281 gtcgcgcgac cgggcctcga cccagcgcag cggcgcgttg aattcgtcgc gggcaatccc
     5341 ggccatgcgc gtcgcttccg cgggcacgct cgcgtccgcc gcgacccagc cgcggccgcc
     5401 ggacgcggcc acggggatgc ccgtctcgcg gccgagccag atcccgtagc gcagccgctt
     5461 gagcgatgcg ttgccgaggt ccgggctggc gtactcggcc ggccgcgcct ccatgccgcc
     5521 gcccagcacg acgatggcca gcgactggcc ggcggcggcg cgcgccttca ggtccgcgcg
     5581 ctgcgcggcg tcgagcggcg caggctcgtc caggaaggac tgctcgaccc agcgtgccgc
     5641 gccgctgcag cagctgatcc aggcgcaggc gcaaccgacg gccaccagcc agcggctcgc
     5701 gcgtggacgc cggcgcgcga gcgcggcgcc cgcgaacgcg agcgcgagga atggcacggg
     5761 cggcagcacc agcgcggcga gcgcgggctt gagggcgccc agtcccgcgg cggtcatcca
     5821 gtcggcaagc atgcgggcga ttgtggcgga gcgccgtggc ggccggaggc cgtgggcgag
     5881 cacgtcatcg ccgacctcga actagggggt accgtactcg tccggcgtcc ggtcgaccgg
     5941 cggcggccgg cgctcagggc gccaggcgct cgcgcgtcca ggcggtgccg tcgaggcgat
     6001 agcgcagccg gtcatgcagg cgcgacttgc ggccctgcca gaactcccac gtgtcgggct
     6061 tgaggcggaa gccgccccag tgcggcggcc gcggcgggtt cagcaggaac ttggcggcgt
     6121 acctggcggc attggccacc agcaccgcac gcgactcgat cacctcgctc tgcggcgagg
     6181 cccaggcccc gatgcgcgaa tcgagcggcc gtgacgcgaa gtacgcgtcc gattccgcat
     6241 cgtcgacgcg ctcgacggcg ccctcgatcc gcaccacgcg ctcgagctcg acccagtgga
     6301 actgcagcgc cgcaagcgga tgcaccgcga gttcgcggcc cttgcggctg tcgtagttgg
     6361 tgaaccagac gatgccgcgc gcgtcgtagc ccttgatgag cacgatgcgc gtcgaggggc
     6421 ggccatcggc ccccacggtc gcgagcgtca tcgcgttcgg ctcgggcacc ttggcggcga
     6481 acgcctcgtc catccaggcc tggaactggc gcagcggatc gagggccgca tcgctttcct
     6541 cgagcgcgtc gcgctcgtag ctcttgcgtt cgtccgcgat ctttttcgaa tgatccatct
     6601 gcacagttta ggggaacgcc ttaggtatac gtcccactgg aacaaaagaa ggccagcaac
     6661 gattcttgag gcacataacc attcattgcg cggccgcctc aggaagacgc cgcgcgccat
     6721 gcgcccatga aaaacgaacc ggccgtcgtc gtgctggctg caggcgcagg gagccgttac
     6781 cagggagcgc gccacaagct cagcgagaag ctgggccttg acagcgtgct cgtgcgaacc
     6841 ctgcgaaatg ccatcgcatc cgagatgtcg gtcgtgctcg tcatctccga tgcgctggta
     6901 tccgaggccc ggggcctgat cgcgcccgag gacatggtcg tcgtcgacgc ccgctcacaa
     6961 acggggtggg gtatgggcga ctcgatcgcc gcgggggtgt cgcagcatgc gagcgccacc
     7021 ggctggctgg tgctgcccgc cgacatgccg ctggtccggc ccggcagcct gcgcgcggtc
     7081 gccgacgcgc tcgaccagca gcccatcgca ttcgcgcagc accgcggtcg ccgcgggcat
     7141 ccggtggggt tcgccgccga gctgttctcc gaactggtga tgctcaaggg cgacgagggc
     7201 gcccgccgcc tgctcgcgcg ctatccgacg gcggcggtcg aactcgatga cccgggcgtg
     7261 ctgttggaca tcgacaccgt cgacgatctc gccgccgcgc agcggcgcat cgacggatcg
     7321 tccgagatcg cgatctgacg gtcgcggcgg tcgctgccga tcgtcgcccg gtgccttcgg
     7381 ttcgccgcga cccgcattga ccgcagtcgc cgccgatcgg cggccatccg tcgccgcacc
     7441 gcatcgccaa ggcgcaaact ccgtacgcg
//
LOCUS       DATCRO010000476         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_758538, whole genome shotgun sequence.
ACCESSION   DATCRO010000476 DATCRO010000000
VERSION     DATCRO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758538"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..759)
                     /locus_tag="VIN75_12920"
     CDS             complement(10..759)
                     /locus_tag="VIN75_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY8975116.1"
                     /translation="MSEIWNSMLSAYWSATEISANLAIFLTLLGALLLGLVVGYERTY
                     HGRAIGMRTYGLVCMASAALTTFVGYPALWYGGHASTAVAVDPTRVVQGIVTGIGFLG
                     AGIIMKDGYNIRGLTTAASVWCACVIGVLVGVGFYGAAVLLAALSVVCMMWGHRLEHW
                     LPAREGVAVVLRFCRGTMPAPGTLEHAVVERGYEIAPGSLSIGMKDGLVEWHFVAMCA
                     KRSKHAPLAELAQLLGSDARIDSFQLAHTRN"
     gene            1033..>3065
                     /locus_tag="VIN75_12925"
     CDS             1033..>3065
                     /locus_tag="VIN75_12925"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8975117.1"
                     /translation="MTISRRSFLETAAALGASLAWVAPARASGVDWKERRDLYPQGVA
                     SGDPDPHSVILWTRRPFDDGARQMLSVEVAEDEAFRRVVAQAPAPVSSASDWTARVLV
                     GGLAPARVYWYRFVDADGNGSRVGRTITAPLPDDPRVVNFAFVSCQDVNEGKLNAYRR
                     MIHEDERAPAAEQLGFVLHLGDFIYEVVEYPDEVKTRYDRTIYEVARIPDGRKVGNFH
                     IPLTLDGYRAIYKGYLADPDLQDARARWPFVAMWDNHEFSWQGWQSIVKAGSFERPGQ
                     SIKVAANQAWFEFLPARVAPPGGSLERFDPPAVQDVPIQQFDADGLGTEPNNLAAINS
                     LQGYRTLRWGRCLDLIITDQHSYRNADCFSDPSLEKLGGEEFMDMFPESGMRILDGGR
                     GYDGGKPPAEIGFNEARVPNPQRGAPPQTILGARQKAWFKDKLRKSTAVWKIWGNSEG
                     ALDSRADPQNLPPGLTRESWPKDASFAALGGGDYGTAYAERAEIYDLVRDARITGFAI
                     VSGDRHSFWAGYAAASLPPAKFEPVGLSFVGGSLSSPGVMEAYEHKLPKDAPLRPLFL
                     ADRGAGAAPAWTYNMLLRHGVRSCLDYAKNFDLKRARALSNPDLAPHLEFVDLGGHGY
                     AKVRLSMNEMRTEFVCTPRPVTRSERSDGGPIRYRVSHTASLWKAGQRPTLKTS"
BASE COUNT          485 a         1126 c         1029 g          425 t
ORIGIN      
        1 gctgatggct cagttgcgcg tgtgcgccag ctggaagctg tcgatgcgcg cgtccgaacc
       61 cagcagctgg gcgagctcgg ccagcggcgc gtgcttgctg cgcttggcgc acatcgcgac
      121 gaagtgccat tcgacgagcc cgtccttcat cccgatggac agcgatccgg gcgcgatctc
      181 gtagccgcgc tccacgacgg cgtgctccag cgtgcccggc gcgggcatcg tgccgcggca
      241 gaaccgcagg acgaccgcga ctccctcgcg tgccggcagc cagtgctcca gccgatgccc
      301 ccacatcatg cagacgaccg acagcgccgc cagcagcacc gcggcgccgt agaagcccac
      361 gcccaccagc acgccgatca cgcacgcgca ccacaccgag gcggccgtcg tcaggccacg
      421 gatgttgtag ccgtccttca tgatgatccc ggccccgagg aagccgatgc cggtcacgat
      481 gccctgcacg acgcgcgtgg gatcgacggc gaccgccgtg ctcgcatggc cgccgtacca
      541 gagcgccggg tagccgacga aggtggtcag cgccgccgac gccatgcaca ccagtccgta
      601 ggtgcgcatg ccgatcgcgc gcccgtggta cgtgcgctcg tagcccacga cgagcccgag
      661 cagcagcgcg cccagcagcg tcaggaagat ggccaggttg gcggagatct ccgtcgccga
      721 ccagtaggcc gacaacatgc tgttccagat ttcgctcatc gcgtccacgt gcccgcgccg
      781 gcaggccctc ccgggctcga tcgtagtcac gccgccgcgt gcaagctgac gccaagcccg
      841 ggccggacgc ctcagacagc ggattccttc acggcgcgcg gcacgggctc ccgtcgggac
      901 gcctcgtcgc cggctgcact tggcacccgc gatgccgtgc gccagccgcg cccggcaccg
      961 cccgtcgcaa agcgtttgac gcgcctgccg ccagaacgtg tgatggcgac ctgtccgcca
     1021 aggagaacga cgatgacgat cagccgccga tccttcctcg agaccgccgc cgcgctcggc
     1081 gcatcgctcg cctgggtcgc cccggcccgg gcctcgggcg tcgactggaa ggaacggcgc
     1141 gacctgtatc cccagggcgt ggcctcgggc gatcccgatc cgcacagcgt catcctgtgg
     1201 acgcggcgcc cgttcgacga cggcgcgcgc cagatgctga gcgtcgaggt cgccgaggac
     1261 gaggcgttcc gccgcgtcgt cgcgcaggcg ccggcgccgg tctcgagcgc ctcggactgg
     1321 accgcgcgcg tcctggtcgg cggcctcgcg ccggcgcgcg tctactggta ccggttcgtc
     1381 gacgccgacg gcaacggcag ccgggtcggc cgcacgatca cggcgccgct gccggacgat
     1441 ccgcgcgtgg tgaacttcgc gttcgtgagc tgccaggacg tcaacgaggg caagctcaac
     1501 gcctatcgcc gcatgatcca cgaggacgaa cgcgcgcccg ctgccgagca gctcggcttc
     1561 gtgctgcacc tgggcgactt catctacgag gtggtcgagt atccggacga ggtcaagacg
     1621 cgctacgacc gcaccatcta cgaggtcgcg cgcattccgg acggccgcaa ggtcggcaac
     1681 ttccacatcc cgttgacgct cgatggctac cgcgccatct acaagggcta cctcgccgac
     1741 cccgacctgc aggacgcgcg cgcccgctgg ccgttcgtcg ccatgtggga caaccacgag
     1801 ttctcgtggc agggctggca gagcatcgtc aaggccggct cgttcgagcg gcccggccag
     1861 agcatcaagg tggcggccaa ccaggcctgg ttcgaattcc tgcccgcgcg tgtcgccccg
     1921 ccgggcggct cgctcgagcg cttcgatcca ccggccgtcc aggacgtccc gatccagcag
     1981 ttcgacgccg acggcctggg caccgagccg aacaacctgg cggcgatcaa cagcctgcag
     2041 ggctaccgca cgctgcgctg gggccgctgc ctggacctga tcatcaccga ccagcacagc
     2101 taccggaacg cggactgctt cagcgatccg tccctcgaga agctgggcgg cgaagagttc
     2161 atggacatgt tccccgagtc cgggatgcgc atcctggacg gcggccgcgg ctacgacggc
     2221 ggcaagccgc cggcggagat cggcttcaac gaggcgcgcg tgcccaaccc gcagcggggc
     2281 gcgccgccgc agacgatcct cggggcccgg cagaaggcct ggttcaagga caagctgcgc
     2341 aagtcgaccg ccgtgtggaa gatctggggc aactcggaag gcgccctgga ttcgcgcgcc
     2401 gatccgcaga acctgccacc gggcctgacc agggaatcgt ggccgaagga cgcctccttt
     2461 gccgcattgg gtggtggcga ctacggcacg gcgtacgccg aacgcgcgga gatctacgac
     2521 ctcgtgcgcg acgccaggat caccgggttc gcgatcgtct cgggcgatcg ccacagcttc
     2581 tgggccggct atgcggccgc cagcctgccg cccgcgaagt tcgagccggt cggcctgagc
     2641 ttcgtcggcg gctcgctcag cagcccgggc gtcatggagg cctacgagca caagctgccc
     2701 aaggatgcac cgctgcgccc gctgttcctc gccgaccgcg gcgcgggcgc cgcgccggcc
     2761 tggacgtaca acatgctgct gcggcacggc gtccgatcgt gcctcgacta cgcgaagaac
     2821 ttcgacctca agcgcgcccg cgcgctgtcc aacccggatc tcgccccgca cctggagttc
     2881 gtcgatctcg gcgggcacgg ctatgcgaag gtgcggctgt cgatgaacga gatgcgcacc
     2941 gaattcgtct gcactcctcg tccggtcacg cgcagcgaac ggtccgatgg cgggccgatc
     3001 cgctaccggg tatcgcacac cgcgtcgctg tggaaggccg ggcagcgccc gacgctgaag
     3061 acatc
//
LOCUS       DATCRO010000477         9350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_972807, whole genome shotgun sequence.
ACCESSION   DATCRO010000477 DATCRO010000000
VERSION     DATCRO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972807"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VIN75_12930"
     CDS             complement(<1..995)
                     /locus_tag="VIN75_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8975118.1"
                     /translation="MLPKLLTQIFGSRNDRLLKQYRRVVEKIASLEAPTAALSDEALK
                     GKTAEFRARLAQGGHTLDDLLPEAFAVVREAGKRVLKMRHFDVQLIGGMTLHNGKIAE
                     MRTGEGKTLMATLPVYLNALGGKGVHLITVNDYLARRDAEWMGRLYNFLGMTVGVNVP
                     GLTREEKQAAYGADITYGTNNEFGFDYLRDNMVYETGDRVQRGLNFAIVDEVDSILID
                     EARTPLIISGQAEDHTELYVRINAVVPALKRQVGEADPRTGEGVIEPGDFTVDEKGHQ
                     VTLTESGHERAEELLAQAGLLVEGASLYDPANITLMHHVYAALRANHLYHRDQHYV"
     gene            complement(1270..2241)
                     /locus_tag="VIN75_12935"
     CDS             complement(1270..2241)
                     /locus_tag="VIN75_12935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8975119.1"
                     /translation="MITHGGMARTRVMNVSPTGLVLSLLMIVIATLALSGAIYHYFFL
                     KAAREGWPVVSQLVKLVVHDESAARDKVMRENLDAMAQKVGDMQARMIKIEAMGDRVS
                     GMAGLKPEDLSGLTSRPKGGGSGGPFVPMNHPGLAELNGALDALAQSAGEQSDVLTLT
                     ESRLLESRLKTLMIPSSRPIDVPVGSGFGFRADPFTGRGALHTGLDFPSPIGTEIHAA
                     AGGVVRTAEMHPEYGLMLEIDHGNGLVTRYGHTSKILVKPGDIVKRGQVVADVGTTGR
                     STGPHLHFEVLVDGVPQDPQRFLAGANARPAIQTAAGQASPLGAEHR"
     gene            2269..2664
                     /locus_tag="VIN75_12940"
     CDS             2269..2664
                     /locus_tag="VIN75_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975120.1"
                     /translation="MAPPGPPKPNDRDAPRQAFYAPLPQPKSVVPLARPLGDSLTKDD
                     TLAGLLARVRASQARLDALADVLPGLLRQHLRAGPLDDDGWTILAANSAVASKLRHLL
                     PTLAETLVAKGWQATSIRVKVQSTHAPGR"
     gene            complement(2729..3034)
                     /locus_tag="VIN75_12945"
     CDS             complement(2729..3034)
                     /locus_tag="VIN75_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975121.1"
                     /translation="MTLHFDWFHAPRWLAHHHVSRAVSLVLGVIGVSLLFSALRVVDA
                     AVSGALIDRHAAAPAGAAMLVAPERLLREQAPSSGPSAVETPTAVEVPEATAEAVSL"
     gene            complement(3098..3838)
                     /locus_tag="VIN75_12950"
     CDS             complement(3098..3838)
                     /locus_tag="VIN75_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975122.1"
                     /translation="MTTYDTPHFATGNSDLRSEPPRPRPASAAPYRTAGVTNEAAAMR
                     EVGQRPDWQPGASKGVPKAAIGAVGAALVGGVALAIVLMAPSNKVVKHVQPDPIAQAR
                     SENNAAQKSVASTDAAVNELDKNAPAAGNSASQAALAAQQASAVAAQAAVPAPVQNVV
                     PDAPPVANAPVAHVPKHVSTPRVAPQHAAPAAAKTDTSADSPAVTPPADVQAPAMQQT
                     PDTPTVAPTAPPTAPTTPPDTPAQAPAQ"
     gene            4124..4288
                     /locus_tag="VIN75_12955"
     CDS             4124..4288
                     /locus_tag="VIN75_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975123.1"
                     /translation="MDNFLAGQARDAKFDPALLSIGDPFEMHADDNLGDEAPFPSTWQ
                     QLDATDAPHE"
     gene            4437..4820
                     /locus_tag="VIN75_12960"
     CDS             4437..4820
                     /locus_tag="VIN75_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975124.1"
                     /translation="MSNLLAFVVEDSPVIQENLIAALEEMAPVKVVGTAVDQAGALSW
                     LSDDGNRCDIAIIDIFLRQGSGMGVLRALHAAGAPFERVVLTNYATADIRRQCLALGA
                     SAVFDKSGEIDALVDHCVARAANGG"
     gene            complement(4893..5525)
                     /locus_tag="VIN75_12965"
     CDS             complement(4893..5525)
                     /locus_tag="VIN75_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975125.1"
                     /translation="MIRVVIVDDHAIVRSGLRQFLSDHVDLRVTGEAADGREALELVR
                     GGDLDVMLMDLSMPDQSGVDALAAIKARRPDLPVLILSGFPETHYATTLLRQGASGYL
                     NKGCDPDEIVKAIRTVAMGRRYITAQVAEMLADGAAGAGDRPPHEQLSERELQVFLRL
                     AKGETVGQIAEGMFLSVKTVSTYRARTLEKLQLETNSDLTYYALKNGLIQ"
     gene            5666..7105
                     /locus_tag="VIN75_12970"
     CDS             5666..7105
                     /locus_tag="VIN75_12970"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY8975126.1"
                     /translation="MSFLRTLETARRSRFAAPVALLLGLAVLLTNEVGHRRALSVIES
                     REALLDARVEVSLLLREVIAAESGQRGYLLTGRPEYKEPYTHAAAQVQAQLTHLAQIY
                     SRWPDRTADVKKLDELTRQRMSELETTIQMFESKAGEGWREVVLTDIGRETMLAIEGQ
                     ARNMARVETTRIDESRELLTETLMFSRIGIAALVVLSLAALLLYARQARRFDDERLER
                     AAQLRFERDRLEVEVERRTRDITQIATHLQTAREDERSRLARELHDELGGLLTAAKLD
                     VARMRSRLSAGPPEVLERMVHLVKTLDEGIALKRRIIEDLRPSSLNNLGLKAALEILC
                     TEFAERSELDVRTDIEELPLDDAGKLTTYRLVQEALTNVAKYASASTVHVGVAAHGGW
                     AVIAVRDDGVGFDPTAARAAAHGLAGMRFRVQSARGQLKIKSRPGQGTTIQARLPLPD
                     HVQDAVDSTMDVASLLGPGAFPKPPANVG"
     gene            7257..7571
                     /locus_tag="VIN75_12975"
     CDS             7257..7571
                     /locus_tag="VIN75_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8975127.1"
                     /translation="MITRLTIAAMLAATALVTATGCAVERHQETVGAYVDDSAVTTKV
                     KAKFAEDPTVSAMAIKVDTMKGVVQLSGFAKSNEERMMAERLARQTNGVQGVRNDIIV
                     KQ"
     gene            complement(7744..7929)
                     /locus_tag="VIN75_12980"
     CDS             complement(7744..7929)
                     /locus_tag="VIN75_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975128.1"
                     /translation="MISAVASPDKLPSSHPAPFDKGWVPQGYIGPVALPGTGRMVFWT
                     GRIAIGLRYAPPERVQH"
     gene            8083..8526
                     /locus_tag="VIN75_12985"
     CDS             8083..8526
                     /locus_tag="VIN75_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975129.1"
                     /translation="MDRSEIITDESVEFMRQPDGRWRWHARTPEAAHVLATELGAPSM
                     LSFKTQFEAAADVAVHADAERDATGRLVMTRDYIRRMVSAIALPCAACADVYFGSVYW
                     HRRDSSGANWGVDIVNGTGDFDGCLECVAVAREDLRRLYSIVDEE"
     gene            8814..>9350
                     /locus_tag="VIN75_12990"
     CDS             8814..>9350
                     /locus_tag="VIN75_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8975130.1"
                     /translation="MTDQPFKVLVVGGGAGGLELACKLGRKWGPDRVALVDANLFHIW
                     KPSLHEVAAGTLDIHQEGLSYQMLAHDNGFSFHYGPMTGLDTATRTLVVGAVVDGTEE
                     VLPERRIGYESLVLAIGSVSNDFGVPGVSEHAFSLDGPHDAERFRLRMLQLLSLVDVR
                     KTHDPSAALDIVIIGGGAT"
BASE COUNT         1409 a         3269 c         3292 g         1380 t
ORIGIN      
        1 acgtagtgct ggtcgcggtg gtacaggtgg ttcgcgcgca gcgccgcgta cacgtggtgc
       61 atcagcgtga tgttggccgg gtcgtacagg ctcgcgccct cgaccagcag gccggcctgc
      121 gccagcagtt cctcggcgcg ctcgtggccc gattcggtca gcgtgacctg gtggcccttt
      181 tcgtcgaccg tgaagtcgcc cggctcgatc acgccctcgc cggtgcgcgg atcggcctcg
      241 ccgacctggc gcttgagcgc cggcacgacg gcgttgatgc gcacgtacag ctcggtgtgg
      301 tcctcggcct ggccgctgat gatcagcggc gtgcgggcct cgtcgatcag gatcgagtcg
      361 acttcgtcga cgatcgcgaa gttcaggccg cgctgcacgc gatcaccggt ctcgtagacc
      421 atgttgtcgc gcaggtagtc gaagccgaac tcgttgttcg tgccgtaggt gatgtcggcg
      481 ccgtacgcgg cctgcttctc ctcgcgcgtg aggccgggca cgttcacgcc caccgtcatg
      541 cccaggaagt tgtacaggcg gcccatccac tcggcgtcgc ggcgcgcgag gtagtcgttg
      601 accgtgatga ggtggacgcc cttgccgccc agcgcgttca ggtagaccgg cagcgtggcc
      661 atcagcgtct tgccttcgcc ggtgcgcatc tcggcgatct tgccgttgtg cagcgtcatg
      721 ccgccgatca gctgcacgtc gaagtggcgc atcttgagca cgcgcttgcc ggcctcgcgc
      781 acgacggcga aggcctcggg cagcaggtcg tcgagcgtgt ggccgccctg cgccaggcga
      841 gcgcggaatt cggccgtctt gcccttgagc gcctcgtcgc tgagcgccgc ggtgggcgct
      901 tcgagcgagg cgatcttctc gacgacgcgc cggtactgct tgagcaggcg atcgtttcgg
      961 ctaccgaaaa tctgggtaag aagcttgggc aacatgcgtg ggttctgggc cggaagacag
     1021 gccacggacg ttgcacggcg gccggttttg gcggagccgg ctgtcagccg cgggcctgcc
     1081 gggggtggca agcgaagtcg ttgcgaaggc cgccggggcg cccttcaccg cgccgcatct
     1141 ggggacgcat cacgcgacgg caagggcctg cccgacggcg atccgcgttt cccccgagga
     1201 aacggggcgg aaaaacggga aagtgtagca tccgggcccc ttcggacccg tacttgcgcc
     1261 gcgcagctcc tatcggtgct cggcgccgag cggagacgcc tggccggcgg cggtctggat
     1321 cgccggcctg gcgttggccc cggccaggaa gcgctgcgga tcctgcggca cgccgtccac
     1381 cagcacctcg aagtgcaggt gcgggccggt ggagcgcccg gtggtgccga cgtcggccac
     1441 gacctggccg cgcttgacga tgtcgcccgg cttgacgagg atcttcgacg tgtggccgta
     1501 gcgcgtcacc aggccgttgc cgtggtcgat ctccagcatc aggccgtact cgggatgcat
     1561 ctcggcggtg cgcaccaccc cgccggcggc ggcgtggatc tcggtgccga tcggcgacgg
     1621 gaaatcgagg ccggtgtgca gcgcgccgcg gcccgtgaac gggtcggcgc ggaagccgaa
     1681 gcccgagccc acgggcacgt cgatcggccg gctgctgggg atcatcagcg tcttcaggcg
     1741 cgattcgagc agccgcgact cggtcagcgt cagcacgtcg ctctgctcgc cggcggactg
     1801 cgccagggcg tccagcgcgc cgttgagctc ggccaggccg ggatggttca tcggcacgaa
     1861 ggggccgccg gagccgccgc ccttggggcg cgaggtcagg cccgacaggt cctcgggctt
     1921 caggcccgcc atgcccgaga cgcggtcgcc catcgcctcg atcttgatca tgcgggcctg
     1981 catgtcgccc accttctgcg ccatcgcgtc caggttctcg cgcatgacct tgtcgcgcgc
     2041 ggcgctctcg tcgtgcacca ccagcttgac gagctggctg acgacgggcc agccctcgcg
     2101 cgcggccttc aggaagaagt agtgatagat ggcgccggac aacgccagcg tggcgatcac
     2161 gatcatcagc agcgacagca cgaggccggt cggactgacg ttcatgaccc gcgtgcgggc
     2221 catgccgccg tgggtgatca ttatttgcat cgctacactc tccaacaaat ggccccgccc
     2281 ggacctccca agcccaacga ccgcgacgcg ccgcggcagg cgttctacgc gcccttgccg
     2341 cagcccaagt ccgtggtccc gctcgcgcgg cccctcgggg acagcctcac gaaggacgac
     2401 acgctggccg gcctgctcgc ccgcgtgcgc gcgtcgcagg cgcgcctgga cgcgctggcc
     2461 gacgtgctgc ccggcctgct gcgccagcac ctgcgcgccg ggccgctgga cgacgacggc
     2521 tggacgatcc tggccgccaa ttcggccgtg gcctccaagc tgcgccacct gctgcccacg
     2581 ctcgcggaaa ctctcgtcgc gaaagggtgg caggctactt caatccgggt gaaggttcaa
     2641 tcgactcacg ccccaggccg gtaaacgaaa gaaaccgcgc aggagcgcgg ttccggggcg
     2701 gagtcggcgc cgaccaaggc gcgctgtttc aaaggctcac ggcctcggcg gtcgcttcgg
     2761 gcacttccac cgccgtgggt gtctccacgg ctgagggacc ggacgacggc gcctgttcgc
     2821 gcagcaatcg ctccggcgcg accagcatcg ccgcaccggc cggcgcggcc gcatgacgat
     2881 cgatcagcgc gcccgagacg gcggcgtcga ccacgcgcag cgcactgaag agcagcgaga
     2941 cgccgatcac gcccagaacg agcgagaccg cccggctgac atggtggtgc gccagccagc
     3001 ggggggcgtg gaaccagtcg aagtgcagcg tcacgaggct ctccttcgaa ggcgcatggg
     3061 ctagcaggga gccgatgcgg gcgtcgcgtt gccggcctta ttgcgccggg gcttgagccg
     3121 gagtatccgg cggcgtcgtc ggagccgtgg gcggcgccgt cggagccact gtcggcgtgt
     3181 caggtgtctg ttgcattgct ggggcctgaa cgtcggctgg cggcgtcacg gcggggctat
     3241 cggcggacgt gtccgtcttt gcggcggccg gcgccgcgtg ctgcggcgcc acccgcggcg
     3301 tcgacacgtg cttgggcacg tgcgcgaccg gcgcgttggc caccggcggc gcgtccggca
     3361 cgacgttctg caccggcgcc ggcaccgcgg cctgcgcggc gacggcggac gcctgctgcg
     3421 cggccagcgc ggcctgcgac gcggagttgc ccgcggccgg cgcattcttg tcgagctcgt
     3481 tgacggccgc gtcggtcgaa gcgaccgact tctgcgcggc gttgttctcg gaccgggcct
     3541 gcgcgatcgg atcgggctgc acgtgcttga ccaccttgtt cgaaggcgcc atcagcacga
     3601 tggccagcgc gacgcccccc accagcgcgg cgccgaccgc gccgatcgcg gccttgggaa
     3661 cgcccttgga ggcgccgggc tgccagtcgg gccgctgccc gacttcgcgc atggcggcgg
     3721 cctcgttcgt gacgccggcg gtgcggtaag gagccgccga agcgggacgg gggcggggcg
     3781 gctccgaacg caggtcggag ttgccggtcg cgaagtgggg agtgtcgtag gtagtcatgg
     3841 ctcccacctt aggccccgta ccggccattc aaaatcggca aacgagccga tcgccggtag
     3901 gccggatccg gccgcgggta ggcgtggacc gacatattcc gcaccgcctg ttacaaacag
     3961 cggcgcgctg gacggtcgcg cagcgtgtgt aggaccgcgc tgacacggcg tccgcttctc
     4021 agcctatgtt cagtcttgcc acgcctgagc aaactggcgg caaggtagac gagcgcccgc
     4081 cagcgcagtc gccacaacgg acacaggctg gtcggaggcg gagatggaca actttcttgc
     4141 aggacaggcg cgcgacgcga aattcgatcc cgcgttgctg agcatcggcg acccgttcga
     4201 gatgcacgcc gacgacaacc tcggcgacga ggcgcctttc ccgtccacgt ggcagcagct
     4261 ggatgccacg gacgctccgc acgagtgact tgagcggaca agtcgcgcgg cgccccggtg
     4321 gcgccgcgac gccgggcgtt gcgcgccggc gtcccgccgg atcggaatcg catccggtcg
     4381 ttgcacaatg tggagcagag ggtggcgccg gcttcgtcgg cggactcgct tgtggcatgt
     4441 cgaacctgtt ggcattcgtc gtcgaggaca gtccggtgat ccaggagaac ctgatcgccg
     4501 cgctggagga aatggccccg gtgaaggtgg ttggcaccgc cgtggaccag gcgggcgccc
     4561 tcagctggct cagcgacgac gggaaccgct gcgacatcgc catcatcgac atcttcctgc
     4621 gccagggttc cggcatgggc gtgctgcgcg cgctgcacgc cgcgggcgcg ccgttcgagc
     4681 gcgtcgtgct caccaactac gccaccgccg acatccggcg ccagtgcctg gccctcggcg
     4741 cgagcgccgt gttcgacaag tcgggcgaga tcgacgcgct ggtcgaccac tgcgtggcgc
     4801 gcgcggccaa cggcggctga gcgccagcgc gaggcgaccc catgcatgcg tccgcacgcg
     4861 ccggaagcgc cgtcccggcg ccccgcggcc cgctactgga tcaggccgtt cttcagggcg
     4921 tagtacgtga ggtcgctgtt ggtctcgagc tgcagcttct ccagcgtgcg cgcccggtac
     4981 gtgctgaccg tcttgacgct gaggaacatg ccctcggcga tctggccgac cgtctcgccc
     5041 ttggccagcc gcaggaacac ctgcagctcg cgctcggaca gctgctcgtg cggcggccgg
     5101 tcgcccgcgc cggcggcgcc gtcggccagc atttcggcaa cctgtgccgt gatgtagcgc
     5161 cggcccatgg ccacggtgcg gatcgccttg acgatctcgt cggggtcgca gcccttgttc
     5221 aggtagccgc tggcgccctg gcgcagcagc gtcgtggcgt agtgcgtctc gggaaagccg
     5281 ctgaggatca gcaccggcag gtccgggcgc cgcgccttga tggccgcgag cgcgtcgacg
     5341 ccgctctggt cgggcatcga caggtccatc agcatcacgt cgaggtcgcc gccgcggacg
     5401 agttcgagcg cctcgcggcc gtcggcagcc tcgcccgtga cgcgcaggtc gacgtggtcg
     5461 gacaggaatt gccggaggcc ggagcgaacg atcgcatgat cgtcgacgat cacaacgcga
     5521 atcatgtagt ctcccgcgga atcgatgaac ttggaatcgg gtcggcgcct tgccgaacgg
     5581 atcggtcggt tcgcctcgag ggtcttgcgg tcgcgttgag ggcaagcggc gtaccgagtc
     5641 attcaagtgt agcggggaac ccgacatgtc attcctgagg acgctggaaa ccgcgcggcg
     5701 cagtcgcttc gcggcccccg tggcgctcct gctgggcctg gccgttctgc tgaccaacga
     5761 ggtcgggcac cggcgggccc tttccgtgat cgagagccgc gaggcgctcc tggacgcgcg
     5821 cgtcgaggtc agcctgctgc tgcgcgaggt gatcgccgcc gaatcgggcc agcgcggcta
     5881 cctgctcacc ggacggcccg agtacaagga gccgtacacg cacgcggcgg cgcaggtgca
     5941 ggcccagctc acgcacctgg cgcagatcta ctcgcgctgg cccgatcgca cggccgacgt
     6001 caagaagctc gacgagctga cgcgccagcg catgtcggag ctcgagacca cgatccagat
     6061 gttcgagagc aaggccggcg agggctggcg cgaggtggtg ctgaccgaca tcggccgcga
     6121 gacgatgctc gccatcgagg gccaggcgcg caacatggcg cgcgtggaga cgacgcgcat
     6181 cgacgagtcg cgcgagttgc tgaccgagac gctgatgttc agccgcatcg gcatcgccgc
     6241 gctcgtggtg ctgagcctgg ccgcgctgct gctgtacgcg cgccaggcgc gccggttcga
     6301 cgacgagcgc ctcgaacgcg ccgcgcagct gcgcttcgag cgcgaccggc tcgaagtgga
     6361 ggtggagcgc cgcacccgcg acatcacgca gatcgccacc cacctgcaga cggcgcgcga
     6421 ggacgagcgc agccgcctcg cacgcgagct gcacgacgag ctcggcggcc tgctcaccgc
     6481 cgccaagctc gacgtcgcgc gcatgcgcag ccggctgtcg gccgggccgc cggaagtgct
     6541 cgaacgcatg gtgcacctgg tcaagacgct ggacgaaggc atcgcgctga agcggcgcat
     6601 catcgaggac ctgcgcccgt cgtcgctgaa caacctgggc ctgaaggcgg cgctcgagat
     6661 cctgtgcacc gaattcgccg aacgcagcga gctcgacgtg cgcaccgaca tcgaggagct
     6721 gccgctggac gacgccggca agctcaccac ctaccgcctg gtgcaggagg cgctgaccaa
     6781 cgtggccaag tacgccagcg cgtccacggt gcacgtgggc gtggcggcgc acggcggctg
     6841 ggccgtcatc gcggtgcgcg acgacggcgt gggcttcgat ccgaccgccg cgcgcgccgc
     6901 ggcgcacggc ctggcgggca tgcgttttcg cgtgcagtcg gcgcgcgggc agctcaagat
     6961 caagtcgcgg cccggccagg gcaccaccat ccaggcccgg ctgccgctgc ccgatcacgt
     7021 gcaggatgcc gtcgattcga cgatggacgt ggcctcgctg ctgggcccgg gcgcctttcc
     7081 gaagcccccc gcgaacgtcg gttaggcctc gcttcgcctg cggcgcgcgt ggatgcgtgc
     7141 acccaccgcc ggtttcgtcc gactcgtcga tgcgccgctg tccgggtgcg tgggcacgcc
     7201 gttcgcccta ccgtggagct accgtctttc cacaacgatt tcgaaggagc acaaccatga
     7261 tcactcgtct cacgatcgcc gccatgctcg cggccaccgc cctcgtcacc gccaccggct
     7321 gcgcggtcga acgccaccag gaaaccgtgg gcgcctacgt cgacgactcc gccgtgacca
     7381 ccaaggtcaa ggccaagttc gccgaggatc cgaccgtcag cgcgatggcc atcaaggtcg
     7441 acacgatgaa gggcgtggtg cagctgtcgg gcttcgccaa gtccaacgag gaacgcatga
     7501 tggccgaacg cctggcgcgc cagaccaacg gcgtccaggg cgtgcgcaac gacatcatcg
     7561 tcaagcagta agcgccctgc agctcgcggc ggccgacccc atgccgccgc cgcggccccg
     7621 acggtggaat gccccgcgac gcggggcatt ttcattggcg gagcggacag cccgaagcgg
     7681 tccgccacct gaagggacgg acggcgcgga gccggcgcgc gacgccgcgc gcggcccgcc
     7741 gactcaatgc tgcacgcgct ccggcggcgc gtagcgcagg ccgatggcga tgcggcccgt
     7801 ccagaacacc atgcgcccgg tgccgggcag cgccacgggg ccgatgtagc cctgcgggac
     7861 ccagcccttg tcgaacggcg cgggatgcga ggacggcagc ttgtcgggac tcgcgacggc
     7921 ggaaatcatg gctcacctcc tgtggggatc gcccctcttc ggcaaacagt gagcccggcc
     7981 gtggcgtgac aggatgtgcc acgggcatcc cccgaattcg agcgccaccg ccgagccaaa
     8041 agcgcctacg atcgcaacgg gaacctgcca gcggagaccc acgtggatcg cagcgagatc
     8101 atcacggacg agtcggtcga attcatgcgc cagcccgacg gacgctggcg ctggcacgcc
     8161 cgcacgcccg aagccgcgca cgtgctcgcc accgagctgg gcgcgccgtc gatgctctcg
     8221 ttcaagaccc agttcgaggc cgccgccgac gtcgccgtgc acgccgacgc cgagcgcgac
     8281 gcgaccggcc gcctcgtgat gacgcgcgac tacatccgcc gcatggtcag cgccatcgcc
     8341 ctgccctgcg ccgcctgcgc ggacgtctac ttcggcagcg tgtactggca tcgccgcgac
     8401 agcagcggcg ccaactgggg cgtcgacatc gtcaacggca ccggcgattt cgacggctgc
     8461 ctcgaatgcg tcgccgtcgc ccgcgaggac ctgcgccggc tgtactccat cgtcgacgag
     8521 gagtgaagcg gcggctcctg tcgcgccgcg ggctggagga agacacagcc gccggtcgcc
     8581 gcaagctgcc gagcacggcg cgaatttcag caattcgcgc aacagcacgc gcaccggtcc
     8641 gaagcgtgtg catccgcgat cctgccctca tcgccccgac ggcacgatcc gccatcggcc
     8701 gccggcagcg tgtcgcgcaa ttgacgcaac cgggcccgag tcgaggggct tccccaatct
     8761 ggaccggcat ccgatggtaa acacctagat gcacacaatg acccagcacg cgaatgactg
     8821 accaaccctt caaggtactc gtcgtcggcg gtggcgccgg cgggctcgaa ctggcttgca
     8881 agctgggccg caaatggggc cccgaccgcg tcgcgctcgt cgacgccaac ctgttccaca
     8941 tctggaagcc ctcgctgcac gaggtggcgg ccggcacgct cgacatccac caggaaggcc
     9001 tgtcgtacca gatgctggcg cacgacaacg gcttctcgtt ccactacggg cccatgacgg
     9061 ggctggacac ggccacgcga acgctggtgg tgggcgcggt ggtcgacggc accgaggagg
     9121 tgctgcccga acgccgcatc ggctacgagt cgctggtgct ggcgatcggc agcgtctcca
     9181 acgacttcgg cgtgcccggc gtctccgagc atgcgttctc gctggacggc ccgcacgacg
     9241 cggagcgctt ccggctgcgc atgctgcagc tgctgtccct ggtggacgtg cgcaagaccc
     9301 acgacccgtc cgccgcgctc gacatcgtca tcatcggcgg gggcgccacc
//
LOCUS       DATCRO010000478         7175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_72502, whole genome shotgun sequence.
ACCESSION   DATCRO010000478 DATCRO010000000
VERSION     DATCRO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72502"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VIN75_12995"
     CDS             complement(<1..925)
                     /locus_tag="VIN75_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975131.1"
                     /translation="MPDTDPTAPVVVLTGASSGIGRATAQRLAERGADIVLAARNVHT
                     LRPVADECQARGARVLAVPTDVSDPDAVAALARLAIERFGRIDVWINNVGVGAVGLFD
                     EVPLALHRRVIESNLIGHMNGAHAALVHFRERRRGTLINMISLGGWVPSPYAAAYSAS
                     KFGLRGFTESIRAEMSALPDVHVCAVCPTFVDTPGVGHGANRVGRHLSPPSPMLDPRE
                     VAEAVVRLIDSPRPTVMLGAVATPARVAHAIAPGLTGRIGRRAMEKALERAGPADVGM
                     GNLFEPSGDTAIDGGYRRPKRLPVGTLAVGAA"
     gene            1017..2081
                     /locus_tag="VIN75_13000"
     CDS             1017..2081
                     /locus_tag="VIN75_13000"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8975132.1"
                     /translation="MTSAALPKPPIEPMLAKIADALPDEAGFQFEPKWDGFRALVFRD
                     ADGLVLQSRDLKPLDRYFPELAQALRAQLPPGCVLDGEIVIATPRGLDFDALQQRLHP
                     AATRVQRLSRETPASFVAFDLLAVDGHDAMALTQAERRVLLEGLLVDATPPLHLTPVT
                     HDRAEAVRWLAQFEGAGLDGVVAKLESAAYEPGKRAMLKIKHARSADCVVAGFRWYKD
                     RTDAIGSLLLGLYDDARTLRHVGVTSSFTMATRQALLQELAPLRQDAAASHPWRASLE
                     ADGQGPGAQSRWSRDKDLSWEPLRPERVCEVRYDHLQGARFRHATTFLRWRPDKPPAD
                     CRYDQLEVTTPYELARVFGA"
     gene            complement(2051..3262)
                     /locus_tag="VIN75_13005"
     CDS             complement(2051..3262)
                     /locus_tag="VIN75_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEY8975133.1"
                     /translation="MNDDAVTLEVGGREVVVTHPGKLLIPTARVRKIDLVQYYVALAD
                     GALRGAGGRPCVLVRHPNGLGAPYFFQKRAPEPRPGWVEVAQLHYPSGRTAQEVVPRH
                     ASDLAWMANLACLELHPHSVRPDDLEHPDELRIDLDPVPGVEWPQIVHVAGVARAVLG
                     DAGLVGWPKTSGSRGIHVLVRIERRWNHDAVRRAALAFAREVERRAPGLATSAWHKEQ
                     REGVFVDYNQNARDRTVCSAWSVRARPDARVSTPVDWRELERCDPAAFTLWTIPARWR
                     KRGDPHAAMDEHAGVLDTLLEWSARDEGAGDGDAPTRFRSRPAQGPLIEAARVAEEAA
                     VQPAIDAWKKAHPEAAARLAPRDVLVDRMRGRSSLSYRVRINLARVPERLRPAPAPAR
                     KAHAPKTRASS"
     gene            complement(3259..4044)
                     /gene="xth"
                     /locus_tag="VIN75_13010"
     CDS             complement(3259..4044)
                     /gene="xth"
                     /locus_tag="VIN75_13010"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8975134.1"
                     /translation="MKIATFNVNGVKGRMPRLLEWLEEERPDIACLQEIRTGDATFPA
                     QPLRAAGYELAWHGQPRHHGVAILARGHAPHERRRGLPGDGADGQARYLEADVGDLLV
                     ASVYLPNGNPVPSPNFDYKLAWMARLDAYARSLLAQGRDTVLAGDFNVVPTNADIYNA
                     GSWRFDAVLQPETRALWERLMSQGWIDAARHLHPGQRDLYTFWVDEEAYRRNAGFRMD
                     FLLLSPGLLPRLRRAGVDTAHRIREKPSDHAPAWIELDDGAGR"
     gene            complement(4050..4202)
                     /locus_tag="VIN75_13015"
     CDS             complement(4050..4202)
                     /locus_tag="VIN75_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975135.1"
                     /translation="MLCLALAVVIAVFGLLDLSAGVGTAMRVLVLSLLGLGLALLGLA
                     GARRRG"
     gene            4345..4605
                     /locus_tag="VIN75_13020"
     CDS             4345..4605
                     /locus_tag="VIN75_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975136.1"
                     /translation="MDDLLDLLQWPAMVVTVLASWWVASTQRRKRAWGFWAYLLSNAL
                     WSAWGWHTSATALVVLQACLAAMNIRGFVKASRAKDDAQRQG"
     gene            4749..5153
                     /locus_tag="VIN75_13025"
     CDS             4749..5153
                     /locus_tag="VIN75_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975137.1"
                     /translation="MEQTTSNPSYTTNEAASGEATPFPVSGQAASATASGAADEARRH
                     DPSAGAAAAPAAHSLLDRVTQRAHEAVDGVSNRISSTLDGLQGGVSSAGDTRDEWMES
                     AREAVRQHPLAALGGAVLVGLALHSLLSSRDR"
     gene            5248..5427
                     /locus_tag="VIN75_13030"
     CDS             5248..5427
                     /locus_tag="VIN75_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEY8975138.1"
                     /translation="MSDDMKNRGPQDRARINLSEEHEVRYWTQALGVDKERLAAAVKA
                     VGSSADRVREHLKSR"
     gene            5438..5992
                     /locus_tag="VIN75_13035"
     CDS             5438..5992
                     /locus_tag="VIN75_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY8975139.1"
                     /translation="MPASAPMGIAALDTWDEQGRLQAVIEAPRGSANKLKFDPRRGVF
                     TLHNVLPLGVAFPFDFGFVPGTLGEDGDPLDVLVLMDEATLPGVVVPCRLVGVIEARQ
                     RPKDDRRAKATRNDRLVAVADRSHRHADARALADLGDGMLAEIERFFVFYNDQKGVRF
                     EPIGRAGKAAVRRLVEAGRRAART"
     gene            complement(5998..6627)
                     /locus_tag="VIN75_13040"
     CDS             complement(5998..6627)
                     /locus_tag="VIN75_13040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY8975140.1"
                     /translation="MPISPSPRPRPQRAGLTEHDRTSDTALVILDMISCWSFPDADAL
                     VRAALDVAPAIARLKRRCIAAGVPVIYANDNSGQWRSDFKFLVRSSLESGGPGADVTR
                     QLEPGPEDFFVLKPKHSAFFATPLEILLDHLRTRRLVITGVAADQCVLNTAADARMRD
                     FEVIVPADCIASMSPARSRRAVDQLRDALQIKVPRASALRLARAPRPRA"
     gene            6768..>7175
                     /locus_tag="VIN75_13045"
     CDS             6768..>7175
                     /locus_tag="VIN75_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8975141.1"
                     /translation="MKALTYHGAKDVRIETVPDPRLVEPDDILLRVTATAICGSDLHL
                     YGGFVPGMMKGDILGHEFMGEVVEVGSAIRTLSPGDRVVVPFPIACGNCFFCTRELYS
                     LCENSNPNAWMAEKVWGYSPSGVYGYSHLLGGYA"
BASE COUNT         1002 a         2613 c         2592 g          968 t
ORIGIN      
        1 ccgccgcacc gacggccagc gtgccgacgg gcagcctctt cggccttcga tagccgccgt
       61 cgatggcggt gtcgcccgag ggctcgaaca ggttgcccat gcccacgtcg gccggcccgg
      121 cgcgctcgag cgccttctcc atcgcgcggc gtccgatgcg gcctgtcagc ccgggggcga
      181 tcgcgtgcgc gacgcgggcc ggtgtggcga cggcacccag catcaccgtc ggccgcggcg
      241 aatcgatcag ccggacgacg gcctcggcca cctcgcgcgg gtcgagcatc ggcgacggcg
      301 ggctcaggtg gcggccgacg cggttcgcgc cgtggcccac gccgggcgtg tcgacgaagg
      361 tcgggcagac ggcgcagacg tgcacgtcgg gcagcgcgga catctccgcg cgaatgcttt
      421 cggtgaagcc gcgcaggccg aacttgctgg ccgagtacgc cgcggcatag ggcgagggga
      481 cccagccgcc gagcgaaatc atgttgatca gcgtgccgcg ccgccgctcg cggaaatgca
      541 ccagtgccgc atgcgcgccg ttcatgtggc cgatgaggtt cgactcgatc acgcggcggt
      601 gcagcgccag cggcacctcg tcgaacaggc ccaccgcgcc gacgccgacg ttgttgatcc
      661 agacgtcgat gcggccgaag cgctcgatgg ccaggcgagc cagcgcagcc acggcgtccg
      721 gatccgacac gtcggtgggc accgccagca cgcgcgcccc acgcgcctgg cattcgtcgg
      781 ccacgggccg cagcgtgtgc acgttgcgtg cggccaggac gatgtcggca ccgcgctcgg
      841 ccaggcgctg tgcggtcgcg cggccgatgc cgctggaggc gccggtcagg acgacgacgg
      901 gcgctgtggg gtcggtgtcg ggcatgggcg ctccttggcg aggatggtcg cggcgctgcg
      961 gcaagcgaga ttccctcgcg gccgcggcgc gcacgcttcc tgccgcggtg ctggccatga
     1021 cgtccgccgc gctgcccaag ccaccgatcg agccgatgct cgccaagatc gccgacgccc
     1081 tgcccgacga ggccggcttc cagttcgagc ccaagtggga cggcttccgc gcgctggtct
     1141 tccgcgacgc ggacgggctg gtgctgcaga gccgcgacct caagccgctc gaccgctact
     1201 tcccggagct ggcgcaggcg ttgcgggcgc agctgccgcc cggctgcgtc ctggacggcg
     1261 agatcgtgat cgcgacaccg cgcggcctgg acttcgatgc gctgcaacag cgcctgcatc
     1321 ccgcggccac gcgcgtgcag cgcctgtcgc gcgagacgcc ggccagcttc gtcgccttcg
     1381 acctgctggc cgtcgacggc cacgacgcga tggcgctcac gcaggccgag cgacgcgtgc
     1441 tgctggaggg cctgctggtc gacgccacgc caccgctgca cctcacgccg gtgacgcacg
     1501 accgcgccga ggccgtccgc tggctcgcgc agttcgaggg cgcggggctg gacggcgtcg
     1561 tggccaagct ggagtcggcc gcctacgagc cgggcaagcg ggccatgctc aagatcaagc
     1621 atgcgcggag cgccgactgc gtcgtcgcgg gcttccgttg gtacaaggat cgcaccgacg
     1681 ccatcggctc gctgctgctg ggcctgtacg acgacgcgag gacgctgcgc cacgtcggcg
     1741 tgacctcgtc cttcacgatg gcgacgcgac aggcgctgct gcaggagctg gcgccgctgc
     1801 gccaggatgc cgcggcatcg catccgtggc gggcgtcgct ggaggccgac ggccaggggc
     1861 cgggcgcgca gagccgctgg agccgcgaca aggatctgtc ctgggagccg ctgcgccccg
     1921 aacgcgtctg cgaggtgcgc tacgaccacc tgcagggcgc gcgcttccgc catgcgacga
     1981 ccttcctgcg ctggcgtccc gacaagccgc cggccgactg ccgctatgac cagctggagg
     2041 tcaccacgcc ctacgagctc gcgcgcgtct tcggcgcatg agccttgcgc gcgggcgccg
     2101 gcgcagggcg caggcgctcg ggcacgcgcg cgaggttgat gcgcacgcgg tacgacagcg
     2161 acgagcggcc gcgcatccgg tcgaccagca cgtcgcgcgg cgcgagccgg gccgccgctt
     2221 cgggatgtgc cttcttccag gcgtcgatcg cgggctgcac cgcggcttcc tcggcgacgc
     2281 gcgcggcctc gatcagcggc ccttgcgccg gccgcgaccg gaacctcgtc ggtgcatctc
     2341 catcaccggc cccctcgtcg cgggccgacc attcgagcag cgtgtcgagg acgcccgcat
     2401 gctcgtccat cgccgcatgc ggatcgccgc gcttgcgcca gcgcgcggga atcgtccaca
     2461 gggtgaaggc tgccggatcg cagcgctcga gctcccgcca gtcgaccggc gtcgacaccc
     2521 gcgcgtccgg acgtgcccgc accgaccacg ccgagcagac ggtgcggtcg cgcgcgttct
     2581 ggttgtagtc cacgaacacg ccctcgcgct gttccttgtg ccaggcgctc gtggccaggc
     2641 ccggggcacg gcgctccacc tcgcgggcga acgccagcgc ggcgcggcgc accgcgtcat
     2701 ggttccagcg ccgctcgatg cgcaccagaa cgtggatgcc gcgcgagccc gaggtcttgg
     2761 gccagccgac gaggccggcg tccccgagca ccgcgcgcgc gacgccggcc acgtgcacga
     2821 tctgcggcca ctcgacgccg gggacggggt cgaggtcgat gcgcagctcg tcgggatgct
     2881 ccaggtcgtc cgggcgcacc gaatgcgggt gcagttccag gcatgcgagg ttggccatcc
     2941 atgccaggtc ggacgcgtgt cgcggaacca cctcctgcgc cgtgcggccc gacgggtagt
     3001 gcagctgcgc cacctcgacc cagccgggcc gcggctcggg cgcgcgcttc tggaagaagt
     3061 acggcgcccc gaggccgttg gggtggcgca cgagcacgca gggccggccg ccggcgccgc
     3121 gcaacgcccc gtcggccagc gcgacgtagt actgcacgag gtcgatcttt ctcacgcgcg
     3181 ccgtcgggat gagcagcttg cccgggtgcg tgacgacgac ctcgcggccg ccgacctcca
     3241 gcgtcacggc gtcgtcgttc atcgcccggc tccgtcgtcg agctcgatcc atgccggcgc
     3301 gtggtcgctc ggcttctcgc gtatacggtg cgccgtatcg acgccggcgc ggcgcaggcg
     3361 cggcagcagg ccggggctga gcagcaggaa gtccatgcgg aaaccggcgt tccggcggta
     3421 cgcctcctcg tcgacccaga aggtgtacag gtcgcgctgc ccggggtgca ggtggcgcgc
     3481 cgcatcgatc cagccctggg acatcagccg ctcccacagc gcgcgtgtct cgggttgcag
     3541 cacggcgtcg aagcgccacg agcccgcgtt gtagatgtcc gcgttggtgg gaacgacgtt
     3601 gaagtcgccc gccagcacgg tgtccctgcc ttgcgcgaga agcgatcgtg cataggcgtc
     3661 caggcgcgcc atccacgcga gcttgtagtc gaagttcggg ctcggcacgg ggttgccgtt
     3721 gggcaggtag acggacgcga cgagcaggtc gcccacatcc gcttccaggt agcgcgcctg
     3781 gccgtccgcg ccgtcgccgg gcaggccgcg gcgccgctcg tgcggcgcgt ggccgcgcgc
     3841 caggatcgcc acgccgtggt ggcgcggctg gccgtgccag gcgagctcgt agccggcggc
     3901 gcgcaagggc tgggcgggga acgtggcatc gccggtcctg atctcctgca ggcaggcgat
     3961 gtccggccgc tcctcctcga gccattcgag caggcgcggc atgcgaccct tgacgccgtt
     4021 gacattgaac gtggcgatct tcatggcgcc tagccgcgac gccgcgcgcc ggccaggccc
     4081 agcagggcca gccccaggcc cagcagcgac agcaccagca cccgcatcgc cgtcccgacg
     4141 ccggccgaca ggtccagcag cccgaagacg gcgatgacca cggccaaggc caggcacagc
     4201 atggcccctc tcatcaacat gatcgacctc cattccaatc agtggtcatc ggcccttggc
     4261 aagggcgatg ccccagccgc gccagcggcc gctggtcgaa cgttcgaggg cggcgcccgg
     4321 gtcggatgtt tgcctgccgg ggcgatggac gatctcctcg acctgctgca atggcccgcg
     4381 atggtcgtga ccgtgctcgc ctcctggtgg gtcgcctcca cgcagcggcg caagcgcgcc
     4441 tggggcttct gggcctacct gctgagcaac gcgctgtggt cggcctgggg ctggcacacg
     4501 tcggcgaccg cgctggtcgt gctgcaggcc tgcctggccg cgatgaacat ccgcggcttc
     4561 gtcaaggcga gccgcgcgaa ggacgacgcg caacggcagg gctgatcacg tccgcccctg
     4621 cgtgatcgca tgctgcgcga ccgaggcgac gatgaccgat cgggcccgca ccttgccgag
     4681 gacgacatcg ggccggccgc gatgacgcgg ccgccacgcc cctcccttcc acgaacctgc
     4741 gaacgaccat ggaacagacc acctccaacc cttcgtacac caccaacgag gccgcctccg
     4801 gcgaagccac gcccttcccc gtcagcggcc aggccgccag cgccacggcc agcggcgccg
     4861 ccgacgaggc gcggcgccac gatccgagcg caggcgcggc cgcggcgccg gccgcgcaca
     4921 gcctgctcga ccgagtgacg cagcgcgccc acgaggcggt cgacggcgtg tccaaccgca
     4981 tctcgtccac gctcgatggc ctgcagggcg gcgtctccag cgcgggcgac acgcgcgacg
     5041 agtggatgga gtcggcacgc gaggccgtgc gccagcaccc gctggccgcg ctcggcggcg
     5101 ccgtgctcgt cggcctggcg ctgcacagcc tcctgtcgtc gcgcgaccgc tgatccacgc
     5161 ggggcgggac gtgcaggtcg tcgaagccgg ccgcctgccg gcatcgcgtt tgctggacgg
     5221 ggacatcgaa aatcccctgg agacgacatg tccgacgaca tgaagaaccg cggcccgcag
     5281 gaccgggccc gcatcaatct ttcggaagag cacgaggtgc gctactggac gcaggcgctc
     5341 ggcgtcgaca aggagcgcct cgccgcggcc gtcaaggccg tcggcagcag cgccgaccgc
     5401 gtgcgcgagc acctcaagag ccgctgaccg ccgcgccgtg cccgcctccg ctcccatggg
     5461 catcgccgcg ctcgacacct gggacgagca gggccggctg caggccgtca tcgaggcgcc
     5521 gcgcggcagc gcgaacaagc tcaagttcga tccgcgccgc ggcgtcttca cgctgcacaa
     5581 cgtgctgccg ctgggcgtcg cgttcccttt cgacttcggc ttcgtgcccg gcaccctggg
     5641 cgaggatggc gacccgctgg acgtgctcgt gctgatggac gaggccacgc tcccgggcgt
     5701 cgtcgtgccc tgccgcctgg tcggcgtgat cgaggcgagg cagcggccca aggacgatcg
     5761 ccgcgcgaag gccacgcgca acgaccgcct cgtcgccgtg gccgaccggt cgcatcgcca
     5821 cgccgacgcg cgcgcgctcg ccgacctggg cgacggcatg ctcgccgaga tcgagcgctt
     5881 cttcgtcttc tacaacgacc agaagggcgt gcggttcgag ccgatcgggc gcgccgggaa
     5941 ggcggccgtg cgcaggctgg tggaagccgg ccggcgcgcg gcgcgcacgt gagcgcgtca
     6001 cgcgcgcgga cgtggcgccc gcgcgaggcg cagcgcgctc gcgcgcggca ccttgatctg
     6061 gagcgcgtcg cgcagctggt cgacggcgcg gcggctgcgc gcggggctca tcgacgcgat
     6121 gcagtcggca gggacgatca cctcgaagtc gcgcatgcgc gcgtcggccg ccgtgttgag
     6181 gacgcactgg tcggccgcga cgcccgtgat caccaggcgc ctggtccgca ggtggtcgag
     6241 caggatctcc agcggcgtcg caaagaatgc ggaatgcttg ggcttgagga cgaagaagtc
     6301 ctcgggcccc ggctccagct ggcgcgtgac gtcggcgccc gggccgcccg attccagcga
     6361 cgagcgcacg aggaacttga agtccgagcg ccactggccg ctgttgtcgt tggcgtagat
     6421 gaccggcacg cccgcggcga tgcagcgccg cttgagccgc gcgatggccg gggccacgtc
     6481 gagcgcggcg cgcaccagcg cgtccgcgtc ggggaaggac cagcagctga tcatgtccag
     6541 gatcacgagc gcggtgtcgc tcgtgcggtc gtgctcggtc aggcccgcgc gctgcgggcg
     6601 cggacgcggc gatggggaga tgggcatggt gcgggcgcga tcgatcggtc gcgtggccga
     6661 gcaaccgctg tgccggcgcg agggcgcaag tcatggacgg ggaacggcca ttgccgaact
     6721 tccggtccag gcgcaatgcg ccacgccttc cccacgacgg agactccatg aaagccctca
     6781 cctaccacgg tgccaaggat gtccgcatcg agacggttcc ggacccgcgg ctcgtcgaac
     6841 ccgacgacat cctgctgcgc gtgaccgcca ccgccatctg cggctccgac ctgcatctct
     6901 acggcggttt cgtcccgggg atgatgaagg gcgacatcct cggccacgag ttcatgggcg
     6961 aggtggtcga agtcggttct gccatccgca cgctaagccc gggcgatcgc gtggtggtgc
     7021 cctttcccat cgcctgcggc aactgcttct tctgcacgcg cgagctctac tcgctgtgcg
     7081 agaactccaa cccgaacgcc tggatggcgg agaaggtctg gggatactcg ccgagcggcg
     7141 tctacggcta ctcgcacctc ctcggcggct acgcc
//
LOCUS       DATCRO010000479        10207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_421818, whole genome shotgun sequence.
ACCESSION   DATCRO010000479 DATCRO010000000
VERSION     DATCRO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421818"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VIN75_13050"
     CDS             complement(<1..711)
                     /locus_tag="VIN75_13050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8975142.1"
                     /translation="MSSEANTPDLATPAYDPSAKQKAQAKTSRIPIKIVPAEQLKKPD
                     WIRVRAATPSSRFYEIKQILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRR
                     CPFCDVGHGRPDPLDPDEPVNLAKTIGALRLKYVVITSVDRDDLRDGGAGHFVECIRR
                     IRELSPETRIEVLVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKQARPGSDYQF
                     SLNLLKKFKAMHPDVPTKS"
     gene            complement(817..1521)
                     /gene="lipB"
                     /locus_tag="VIN75_13055"
     CDS             complement(817..1521)
                     /gene="lipB"
                     /locus_tag="VIN75_13055"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8975143.1"
                     /translation="MISPAVDAPIHSPELRSLGRVPYEPTVDAMKAFTAARAPDTPDE
                     IWVVEHEPVFTQGLAGKAEHVLDAGDIPIVQTNRGGQVTFHGPGQVVAYPLVDLRRLG
                     IYVKEYVYRLEEAVLRTLADLDVTGHRVPGAPGIYVRLDDPFGHAALVHDRPVSDPFR
                     GLGKIAALGVKVSNHCTYHGVALNVSMDLQPFGRINPCGYAGLKTVDLATLGVVADRA
                     TVADVLGRRLRAQFTR"
     gene            complement(1674..2159)
                     /locus_tag="VIN75_13060"
     CDS             complement(1674..2159)
                     /locus_tag="VIN75_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975144.1"
                     /translation="MHLTRNETEKCVMARIVRQKKLHQKNFTLKQYGTWTKAEAAARK
                     WVKATLAVLPPPIGMKNRMTSRNTSGVVGVRLVHAVRRREDKEYPDWRWIAFWPGCPN
                     SGGIGWSVNKYGDEHAFVLACLARKLETVDRASIDTEFETFRGSRDYKRLLKLKQITP
                     A"
     gene            complement(2193..2468)
                     /locus_tag="VIN75_13065"
     CDS             complement(2193..2468)
                     /locus_tag="VIN75_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEY8975145.1"
                     /translation="MQEIPPEQSLITYPSAFPIKVMGPHAEGFVEAIVAVARQFDPEF
                     DHTKVETRPSKGGNYLGITITVTATSRPQLDELYRTLTAHPMVKIVL"
     gene            complement(2484..3374)
                     /locus_tag="VIN75_13070"
     CDS             complement(2484..3374)
                     /locus_tag="VIN75_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY8975146.1"
                     /translation="MTALPDSLCYLNGEFLPLNQAKVSVMDRGFLFGDGAYESIPVYQ
                     RRLFRFDDHLARLERSLGKLEIEADLDPETWLERIRKVVGAHPSADQLVYLQVTRGVA
                     PRSHLMPAGLEPTVFVTTSERSHPTPAQRVQGLACVTARDFRWERGDIKSISLLGNVL
                     ARKISADQAADETILLRDDGSGGPWLTEGASSNVWIVHEGALLGPAPDEHVLAGVRIE
                     LLRELCEETGIAFNLRPVSEADVRAADEVLLSSASKEVIAVTRLDGEQVGHGALRGRP
                     GPVYARLHEAYQRAIQSQSI"
     gene            complement(3474..4625)
                     /locus_tag="VIN75_13075"
     CDS             complement(3474..4625)
                     /locus_tag="VIN75_13075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8975147.1"
                     /translation="MKRLLSFLALAAACCAALGEALPAPEIAARQYLVVDMNASQVLA
                     ERGADTSVDPASLTKLMTAYPVFVALREHKLALDQKLPVSPRALAERRGGAPLMFVDP
                     TMTPTVDELLQGLVVDSGNDAAVALAEAVGGNVDAYVAMMNRQAQAWGLKNSTFRNVT
                     GSTETGHRSTPRDLAAIAQHILRDFPEYAHYYAQRDFKFNNIRQENHNLLLQRDPSVD
                     GMKTGYTEAAGYCLLATAQREFPNGKRRLLALVMGADSVQGRANETQKLLNWGFTAFD
                     DVRLVEAGKPVGAPVPVWKGSQAQVGLGSLEAVYVAVPKGEGDRLKTLVERTDPLVAP
                     LAKGQRVGSLKISTASGTAIATIPLTVLEDVPQAGLFGRAWDAIRLWIK"
     gene            complement(4639..5256)
                     /locus_tag="VIN75_13080"
     CDS             complement(4639..5256)
                     /locus_tag="VIN75_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8975148.1"
                     /translation="MNTFTKRGEIAGPAGALSVAVDTTTDLPSRGLAVICHPHPLQGG
                     TMDNKVVTTLARALVQRGWRVVRFDYRGVRQSAGSWDEGRGEVDDALAVVAAHRVAGE
                     PLVLGGFSFGGYVAAAAAAKLPESDKAQRMVLVAPSTIKHDAPPVAVETLLVHGEADD
                     VVPLSASMDWARPQGLPVVVVPGAGHFFHGQLMILKALVLRHFAE"
     gene            complement(5270..5593)
                     /locus_tag="VIN75_13085"
     CDS             complement(5270..5593)
                     /locus_tag="VIN75_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY8975149.1"
                     /translation="MYYKHHIFFCLNERKPGEDSCAHVGAQAGFDHCKSRIKSAGLSG
                     PGGIRVNKAGCLDRCAGGPVAVVYPDETWYTYVDQADIDEIVDSHLVHGKPVERLLLA
                     PNVGR"
     gene            complement(5638..6819)
                     /locus_tag="VIN75_13090"
     CDS             complement(5638..6819)
                     /locus_tag="VIN75_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY8975150.1"
                     /translation="MDKATRAPARIPRLPASASAPPRESSALLLAVVFTGLVIYASLY
                     PFTGWTWPAGQPLSALLVLRWPRYHLGFDDWSNFVGYMPLGALLYASALRLGRRPLAA
                     GALAVVLSSTLSWSMEFTQHFLPGRYSSLMDWALNSLGAAAGLSLAVAVQSAGAGVRW
                     STVRENWFVPHSGSTMALLLLWPVGFLYPRNVPFCQGITWERLQEAAYDLLSDHPLAP
                     WFEWLWASLGENTGTMSKAAEAFAVALGLLGPCLLAFAISRAGWRRAALVAAAVAVGL
                     AANTLSAALNFGPVHALGWVTSTVPAGLTLGAVLALACVFIGQRPAAALGLVALSINV
                     ALVAQAPSDPYYAESLQQWEQGRFIHFHGLAQWVGWLWPFAALAGLFRRVVRNDAPAP
                     A"
     gene            complement(6849..7286)
                     /locus_tag="VIN75_13095"
     CDS             complement(6849..7286)
                     /locus_tag="VIN75_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8975151.1"
                     /translation="MSLLYPWVKALHLVFVASWFAGLFYLPRIFVNLAQVPSDSHAER
                     ERLLLMARKLYRFASFLMVVAILLGMTLVAMAPALFHTGFWLHAKLLLVVVAIGYHHM
                     CRSLLRSFEKLENRRRERWFRVFNEMSVLLFAAIVILVVVKPF"
     gene            7575..8579
                     /gene="hemB"
                     /locus_tag="VIN75_13100"
     CDS             7575..8579
                     /gene="hemB"
                     /locus_tag="VIN75_13100"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8975152.1"
                     /translation="MSHLPPFPASRPRRMRRDAFSRELMREHRLSPGNFILPVFVIDG
                     QNQVQDIPSMPGVQRMTIDRLLAVAGEALSLGVPALALFPAIEPSLKTPDGAEALNPQ
                     GLVPRTVRELKKRYPELGVMTDVALDPYTSHGQDGLLDETGYIVNDPTVALLTRQARV
                     QAEAGVDIVAPSDMMDGRIGAIRTMLESDGHLHTRIMAYSAKYASAFYGPFRDAVGSA
                     ANLGKSNKKVYQMDPGNSDEALREVALDIAEGADMVMVKPGMPYLDIVRRVKDEFRMP
                     TYVYQVSGEYAMIKGAGLNGWVDGEAVMMESLLAFRRAGADGVLTYFALEAARILKRG
                     "
     gene            8679..>10207
                     /locus_tag="VIN75_13105"
     CDS             8679..>10207
                     /locus_tag="VIN75_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8975153.1"
                     /translation="MTFPDHDRDFDLEVSRRRLLAAAGVASALGLVPFTGARAASATQ
                     AAPAHRPEGDPADTPPVAGLHIQFGADASSEVTVSWHTLQPVRDARVIVGRMDGHFEQ
                     TVPARTVSYTDAKAKLVVHAHHARIERLAPDSQYLYGALHDGAEPDFGTFRTAPRGRA
                     RFTFTSFGDQGTPTLGKRFVAPAGVTLANPPFVNDNLGSPAAGDTVLGVERLRPLFHL
                     FNGDLCYANLADDRVRTWWDFWNNNTRSARHRPWMPSPGNHENELGNGPIGYQAYQTY
                     FSVPEADGQTDVTRGLWYAFSAGSVRVVSIANDDVVYQDGGNSYVRGYSGGAQKAWLE
                     RELAGARRLPGIDWVVVCMHQVAISTADKFNGADLGIREEWVPLFDRHGVDLVVCGHE
                     HHYERSHPIRGHEGNPTLTPLPQATATDHVDTRRGTVHMVIGGGGTSVPSNQLFFDPP
                     QCRVITAVGSPDPTTGKRPPVYVTEHAPWSAVRNAAHSYGFAAFEVDPGTRAGGTTTI
                     KV"
BASE COUNT         1596 a         3546 c         3551 g         1514 t
ORIGIN      
        1 gctcttggtc ggcacgtcgg gatgcatcgc cttgaatttc ttgagcaggt tcaggctgaa
       61 ctggtagtcg ctgccggggc gggcctgctt gtacaggcgc ggcgcggtct cgaggttgtg
      121 gttcatcacg tcgggcggcg cggccttgag gatctccagc gcgcggtcgt cgcggccgcg
      181 gaagtcgggc accagcacct cgatgcgcgt ctcgggcgac agttcgcgga tccggcggat
      241 gcactcgacg aagtggccgg cgccgccgtc gcgcaggtcg tcgcggtcga cgctggtgat
      301 caccacgtac ttcaggcgca gcgcgccgat cgtcttggcc aggttgaccg gttcgtcagg
      361 atcgagcgga tcggggcggc cgtggccgac gtcgcagaac gggcagcgcc gcgtgcactt
      421 gtcgcccatg atcatgaacg tggccgtgcc cttgccgaag cactcgccga tgttggggca
      481 ggacgcctcc tcgcacaccg tgtgcagctt gtgctcgcgc aggatctgct tgatctcgta
      541 gaagcggctg ctgggggtgg cggcgcggac gcggatccag tccggcttct tcagctgctc
      601 ggccggcacg atcttgatcg ggatgcgcga ggtcttggcc tgggccttct gcttggcgct
      661 gggatcgtag gcgggcgtcg cgaggtcggg ggtgttggct tcggaggaca ttgcagtgcg
      721 gcggcgaccc ggcgggacgt cgcgttggag cggcaagcgg agggctcgac acccgagttg
      781 gggctgcggc ggcgcctttc cagcgtcgtg gacggctcaa cgcgtgaact gcgcgcgaag
      841 cctgcggccc agcacgtcgg ccaccgttgc ccggtcggca acgacgccca gtgtagcgag
      901 atcgacggtt ttcagcccgg cataaccgca cgggttgatt cgaccaaagg gttgcaaatc
      961 catcgagacg ttcagggcaa caccgtgata cgtgcagtga ttgctcactt tgacgcccag
     1021 cgccgcgatc ttgcccaggc cccggaacgg gtcggagacg ggccggtcgt gcaccagcgc
     1081 cgcatggccg aacggatcgt ccagccgcac gtagatgccc ggcgccccgg ggacgcggtg
     1141 gcccgtcacg tcgaggtcgg ccagcgtgcg cagcacggcc tcctcgagcc ggtacacgta
     1201 ttccttgaca tagatgccca gccggcgcag gtcgaccagc gggtaggcga ccacctgccc
     1261 aggcccgtgg aaggtcacct ggccgccgcg gttggtctgc acgatgggaa tgtcgccggc
     1321 gtcgagcacg tgctcggcct tgccggccaa gccttgggtg aacacgggct cgtgctcgac
     1381 gacccagatc tcgtcgggcg tgtcgggggc gcgcgcggcc gtgaaggcct tcatggcgtc
     1441 cacggtcggt tcgtacggca cgcggcccaa cgaacgcagc tcgggagaat ggatcggggc
     1501 gtcgaccgcc ggggaaatca tcgaggtggg gccttttctg gctggtcatt ccgcgcgcag
     1561 tcgcggaatc cacccctgca aaggtgcttg ttcatgcgcg tggatcctgc gactgcgcgc
     1621 aggatgacaa gctcggcgga tgctcgattt tagggcagcc atcggcccga cgctcaggcc
     1681 ggggtgatct gcttgagctt gagcaggcgc ttgtagtcgc gcgagccgcg gaaggtctcg
     1741 aactcggtgt cgatcgaggc gcggtcgacc gtctcgagct tgcgcgcgag gcaggccagc
     1801 acgaacgcgt gttcgtcgcc gtacttgttc accgaccagc cgatgccgcc cgagttgggg
     1861 cagccgggcc agaaggcgat ccagcgccag tccggatatt ccttgtcctc gcggcgtcgc
     1921 acggcgtgga ccaggcgcac gcccaccacg ccggacgtgt tgcggctggt catccggttc
     1981 ttcatgccga tcggtggcgg caggacggcc agggtcgcct tgacccactt cctggctgcg
     2041 gcctcggcct tggtccaggt gccgtactgc ttcagcgtga agttcttctg atggagtttc
     2101 ttttgccgga cgatgcgtgc catcacgcac ttctcggtct cgttcctggt gagatgcatg
     2161 aagcctctgc gagtacttcc ctcacccgtg catcacagaa cgatcttgac catcggatgc
     2221 gcagtcaatg tccggtacag ctcatccagc tgcggacggc tggtggctgt gaccgtgatc
     2281 gtgatgccca ggtaattacc ccccttgctg ggtctggtct ccaccttcgt gtggtcgaac
     2341 tccggatcga actgcctggc cactgcaacg atcgcctcga cgaaaccttc ggcatgcggg
     2401 cccatcacct tgatggggaa tgcgctgggg tacgtgatca gcgactgttc gggaggaatt
     2461 tcttgcatgg cggccattac cgttcaaatg gactgcgact gaatcgcacg ttggtaagcc
     2521 tcgtggagac gggcataaac cggacccggc cgaccgcgca acgcgccgtg gccgacctgc
     2581 tcgccgtcca gacgcgtgac ggcgatgact tccttggagg cggaactcag gagaacctcg
     2641 tcggccgcgc gcacgtcggc ctcgctgacc gggcgcaggt tgaacgcgat cccggtctcc
     2701 tcgcacagct cgcgcaacag ctcgatgcgc acgccggcca ggacatgttc gtcgggcgcc
     2761 ggccccagca gcgcgccctc gtggacgatc cagacgttgc tggacgcgcc ctcggtcagc
     2821 cacggcccgc cggagccgtc gtcgcgcagc aggatcgtct cgtcggccgc ctggtcggcg
     2881 gagatcttgc gggcgagcac gttgccgagc agcgagatgc tcttgatgtc gccgcgttcc
     2941 cagcggaagt cgcgcgcggt cacgcacgcg aggccctgca cgcgctgcgc cggcgtcggg
     3001 tgcgatcgtt cgctggtggt gacgaacacc gtgggctcca ggcccgcggg catcaggtgc
     3061 gagcgcggcg cgacgccgcg cgtgacctgc aggtagacca gctggtcggc cgacggatgc
     3121 gccccgacca ccttgcggat gcgctcgagc caggtctccg gatcgaggtc ggcctcgatc
     3181 tcgagcttgc ccaggctgcg ctccaggcgg gccagatggt cgtcgaagcg gaacagccgc
     3241 cgctggtaga cggggatcga ctcgtaggca ccgtcgccga acaggaaccc gcggtccatc
     3301 accgagacct tggcctggtt caacggcagg aattccccgt tgagatagca cagcgaatcg
     3361 ggcagggcgg tcatggtcgt tgctcctggg cgagccggac gcgggtggcg ccggacactg
     3421 gaaacccaag caccggtgcc gtggggacgg cgaccggtgc gcaggtcact agcctacttg
     3481 atccagaggc ggatggcatc ccaggcgcgg ccgaacaggc ccgcctgcgg cacgtcctcc
     3541 aggaccgtga gcgggatcgt ggcaatcgcg gtaccggagg cggtactgat cttgagcgag
     3601 ccgacgcgct ggcccttggc gagcggcgcc accagcggat cggtgcgctc gaccagcgtc
     3661 ttgagccggt cgccctcgcc cttgggcacg gcgacgtaga cggcctccag gctgcccagg
     3721 ccgacctgcg cctggctgcc cttccacacc ggcaccggtg cgcccacggg cttgcccgcc
     3781 tccacgaggc gcacgtcgtc gaacgcggtg aagccccagt tgagcagctt ctgcgtctcg
     3841 ttggcgcggc cctggacgga gtcggcgccc atgaccagcg ccagcagccg ccgcttgccg
     3901 ttggggaact cgcgctgcgc ggtcgcgagc aggcagtacc cggcggcctc ggtgtagccg
     3961 gtcttcatgc catcgacgct cgggtcgcgc tgcagcagca ggttgtggtt ctcctggcgg
     4021 atgttgttga acttgaagtc gcgctgcgcg tagtagtggg cgtactcggg gaaatcgcgc
     4081 aggatgtgct gcgcgatcgc cgccaggtcg cgcggcgtgc tgcggtggcc cgtctcggtc
     4141 gacccggtca cgttcctgaa cgtgctgttc ttcaggcccc aggcctgcgc ctggcggttc
     4201 atcatggcca cgtaggcgtc gacattgccg cccacggcct cggcgagcgc cacggcggcg
     4261 tcgttgcccg agtcgacgac caggccctgc agcagttcgt ccaccgtcgg cgtcatcgtg
     4321 gggtcgacga acatcagcgg ggcgccgccc ctgcgctcgg ccagcgcgcg cggcgacacc
     4381 ggcagcttct ggtccagcgc gagcttgtgt tcgcgcaagg cgacgaacac cggataggcc
     4441 gtcatcagct tggtgagcga ggcggggtcg accgaggtgt cggcgccgcg ttcggccagc
     4501 acctggctcg cgttcatgtc gacgacgagg tactggcgcg ccgcgatctc gggggcgggc
     4561 agcgcctcgc ccaaggcggc gcagcaggcg gcggccagcg cgaggaagga aagcagtctt
     4621 ttcatggaga cgccgatgtc actcggcaaa gtggcgcagc acgagcgcct tcaggatcat
     4681 cagctggccg tggaagaaat gacccgcccc cggcacgacg accaccggca gcccctgcgg
     4741 gcgcgcccag tccatcgacg ccgacagcgg cacgacgtcg tcggcctcgc cgtgcacgag
     4801 cagcgtctcg accgccaccg gcggcgcgtc gtgcttgatg gtggacgggg cgacgagcac
     4861 catcctctga gccttgtcgg actccggaag tttcgcggcc gccgccgcgg cgacgtagcc
     4921 gccgaacgag aagcccccga gcaccagcgg ctcgcccgcc acgcggtgcg ccgcgacgac
     4981 ggccagtgcg tcgtcgacct cgccgcggcc ttcgtcccaa ctgccggccg actggcgcac
     5041 gccgcggtag tcgaagcgca ccacgcgcca gccgcgctgc accagcgcgc gggcgagcgt
     5101 ggtgaccacc ttgttgtcca tcgtgccgcc ctgcagcggg tgcggatggc agatgacggc
     5161 caggccgcgc gagggcaggt cggtggtggt gtcgaccgcg acgctcaatg cgcccgccgg
     5221 cccggcgatc tcgccgcgct tggtgaaggt gttcatgtgc gtgcccgcct tagcggccga
     5281 cgttcggcgc cagcagcagc cgttcgaccg gcttgccgtg gaccaggtgc gagtcgacga
     5341 tctcgtcgat gtcggcctgg tcgacgtagg tgtaccaggt ctcgtcgggg tagacgacgg
     5401 ccaccggtcc gccggcgcag cggtccaggc agcccgcctt gttcacgcgg atgccgcccg
     5461 ggccggacag gccggcggac ttgatgcgcg acttgcagtg gtcgaagccg gcctgagcac
     5521 cgacgtgcgc gcagctgtcc tcgccgggct tgcgctcgtt gaggcaaaag aagatgtggt
     5581 gcttgtagta catgcgccca ttgtaggaag gcgcgacggc cgcgttgcgg cccagggtca
     5641 ggccggcgcg ggcgcgtcgt tgcgcaccac gcgccggaac agcccggcca gcgccgcgaa
     5701 cggccacagc cagcccaccc actgcgccag cccgtggaaa tggatgaagc ggccctgctc
     5761 ccactgctgc aggctctcgg cgtagtacgg gtcggacggt gcctgggcca cgagcgcgac
     5821 gttgatggag agcgctacca ggcccagcgc cgccgccggc cgctggccga tgaagacgca
     5881 ggccagtgcc agcacggcgc ccagcgtcag cccggccggc acggtcgacg tgacccagcc
     5941 cagcgcatgc accggcccga agttcagcgc ggccgacagc gtgttggcgg ccaggccgac
     6001 ggccaccgcc gcggccacca gcgccgcgcg gcgccagccg gcgcgcgaga tggcgaacgc
     6061 gagcaggcac ggcccgagca gcccgagcgc gacggcgaac gcctccgcgg ccttggacat
     6121 cgtgcccgtg ttctcgccca ggctggccca cagccattcg aaccacggcg ccagcgggtg
     6181 gtcgctgagc aggtcgtacg cggcctcctg cagccgctcc caggtgatgc cctggcagaa
     6241 cggcacgttg cgcggataca ggaagccgac cggccagagc agcagcagcg ccatcgtcga
     6301 cccgctgtgc ggcacgaacc agttctcgcg cacggtcgac cagcgcacgc cggcccccgc
     6361 cgactgcacc gcgacggcca ggctgagccc cgcggccgcg ccgagcgaat tcagcgccca
     6421 gtccatcagc gaggaatagc gtcccggcag gaagtgctgg gtgaactcca tggaccacga
     6481 cagcgtcgac gacagcacga ctgccagggc cccggccgcc agcggccgcc ggcccagccg
     6541 cagcgcgctg gcgtacagca gcgccccgag cggcatgtag ccgacgaagt tcgaccagtc
     6601 gtcgaagccc aggtggtagc gcggccagcg cagcacgagc agggccgaca gcggctggcc
     6661 cgccggccag gtccagccgg tgaacggata caggctcgcg tagatcacca ggccggtgaa
     6721 caccaccgcg agcagcagcg ccgagctctc gcgcggaggg gcggacgcgc tcgcgggcag
     6781 gcgcgggatg cgcgcgggcg cgcgggtggc cttgtccatc gccagtgacg cccgcgccgt
     6841 gccgccgctc agaacggctt gacgacgacc aggatgacga tggccgcgaa cagcagcacc
     6901 gacatctcgt tgaacacgcg gaaccagcgc tcgcggcggc ggttctccag cttctcgaag
     6961 gagcgcagca ggctgcggca catgtggtgg tagccgatcg ccacgaccac cagcagcagc
     7021 ttggcatgca gccagaagcc ggtgtggaac aaggccggtg ccatggcgac cagcgtcatg
     7081 cccagcagga tcgccaccac catcaggaag ctggcgaagc ggtagagctt gcgcgccatc
     7141 aggagcagcc gctcacgttc ggcatggctg tcggagggca cctgcgccag gttcacgaag
     7201 atgcgcggga ggtagaacag cccggcgaac cagctggcca cgaagacgag gtgcagggcc
     7261 ttgacccagg ggtagaggag gctcatcgcc gcattatgtg cggccatccc ggcgcccccg
     7321 ggcgcggcca ggggaatggg ccgtcggtgg ccccccggaa aagaaagagc cccggtatcc
     7381 gcaagggacg accgaggctg caaagccttg acgctgtcat cacgcaaggc acctgctcag
     7441 ggaggaaaag caggggacaa tatcctcacg gacaccgtcc gcattggaat gtagcagaac
     7501 atccgagctg cgcgacaggg ttcgcccgct ttgtgggcct taaccccaca ttttttgacc
     7561 gagagagctc cgacttgtcc cacctccctc cctttcccgc cagccggccg cgccgcatgc
     7621 ggcgggacgc cttcagccgc gagctgatgc gcgagcaccg gctgagcccg ggcaacttca
     7681 tcctgccggt gttcgtcatc gacggccaga accaggtgca ggacatcccc agcatgcccg
     7741 gcgtgcagcg catgacgatc gaccgcctgc tggcggtggc aggggaagcg ctgtcattgg
     7801 gcgtgccggc gctggcgctg tttcccgcca tcgagccgtc gctcaagacg cccgacggcg
     7861 ccgaggccct gaacccgcag ggtctggtgc cgcgcaccgt gcgcgagttg aagaagcgct
     7921 atccggagct cggcgtgatg accgacgtcg cgctcgatcc gtacacctcg cacggccagg
     7981 acggcctcct ggacgagacc ggctacatcg tcaacgaccc gacggtggcg ctgctgacgc
     8041 gccaggcgcg cgtgcaggcc gaggccggcg tcgacatcgt cgcccccagc gacatgatgg
     8101 acggccgcat cggcgcgatc cgcacgatgc tcgaatcgga cggccacctg cacacgcgca
     8161 tcatggccta cagcgccaag tacgcgagcg ccttctacgg cccgttccgc gacgccgtgg
     8221 gttcggccgc caacctcggc aagagcaaca agaaggtcta ccagatggac cccggcaact
     8281 ccgacgaggc gctgcgcgag gtcgcgctcg acatcgccga gggcgccgac atggtcatgg
     8341 tcaagccggg catgccttac ctggacatcg tgcgccgcgt gaaggacgag ttccgcatgc
     8401 cgacctacgt ctaccaggtc agcggcgagt acgccatgat caagggcgcc gggctcaacg
     8461 gctgggtcga cggcgaggcc gtcatgatgg agtcgctgct ggcgttcagg cgtgccggcg
     8521 cggacggcgt gctcacgtac ttcgcactgg aagcggcgcg catcctgaaa cgcggctgac
     8581 gccgccggcg cggtcgcgcg gcggtcatcc gcgcgatgca cgcctgtcat gttggcggcg
     8641 ttcaatgggt cgctccaggg actcgaaacg ccgccgccat gaccttcccc gatcacgatc
     8701 gcgacttcga cctcgaagtc agccgccgcc gcctgctcgc cgccgcgggc gtggccagcg
     8761 ccctcggcct cgtgccgttc accggtgcgc gcgccgccag cgccacgcag gccgcgcccg
     8821 cccatcgccc cgagggcgac ccggccgaca cgccgcccgt cgccggcctg cacatccagt
     8881 tcggcgcgga cgcgtcgagc gaggtgaccg tgtcctggca cacgctgcag ccggtgcgcg
     8941 acgcgcgcgt catcgtcggc cgcatggacg gccatttcga gcagacggtg ccggcgcgca
     9001 cggtcagcta caccgacgcg aaggccaagc tcgtcgtcca tgcgcaccac gcccgcatcg
     9061 agcgcctggc gcccgactcg cagtacctgt acggcgcgct gcacgacggt gccgagccgg
     9121 atttcggcac gttccgcacc gccccgcgcg gccgcgcccg cttcacgttc accagcttcg
     9181 gcgaccaggg cacgcccacg ctgggcaagc gcttcgtggc gccggccggc gtcacgctgg
     9241 ccaacccgcc gttcgtcaac gacaacctcg gctcgccggc cgccggcgac accgtcctgg
     9301 gcgtcgagcg cctgcgcccg ctgttccacc tgttcaacgg cgacctctgc tacgccaacc
     9361 tcgcggacga ccgcgtccgc acgtggtggg acttctggaa caacaacacg cgcagcgcgc
     9421 gccatcgccc gtggatgccg tcgcccggca accacgagaa cgaactcggc aacggaccga
     9481 tcggctacca ggcctaccag acctacttct cggtgcccga ggccgacggc cagaccgacg
     9541 tcacgcgtgg cctctggtac gcgttcagcg cgggctcggt gcgcgtggtc agcatcgcca
     9601 acgacgacgt cgtctaccag gacggcggca acagctacgt gcgcggctac tcgggcggcg
     9661 cgcagaaggc ctggctcgag cgcgagctcg ccggcgcccg ccgcctgccc ggcatcgact
     9721 gggtggtggt gtgcatgcac caggtggcga tctccaccgc cgacaagttc aacggcgcgg
     9781 acctgggcat ccgtgaggaa tgggtgccgc tgttcgaccg ccacggcgtc gacctggtcg
     9841 tgtgcggcca cgagcaccac tacgagcgct cgcacccgat ccgcggccac gagggcaacc
     9901 ccacgctgac gccgttgccg caggccaccg cgaccgacca cgtcgacacg cgccgcggca
     9961 ccgtgcacat ggtgatcggc ggcggcggca cgtcggtgcc gtcgaaccag ttgttcttcg
    10021 atccgccgca atgccgcgtg atcactgcgg tcggttcgcc cgacccgacg acgggcaagc
    10081 gccccccggt ctacgtcacc gaacacgcac cctggtcggc cgttcgcaac gcggcgcact
    10141 cgtacggctt cgcggccttc gaggtcgatc cgggcacgcg cgcgggcggc acgacgacga
    10201 tcaaggt
//
LOCUS       DATCRO010000480         7997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_522643, whole genome shotgun sequence.
ACCESSION   DATCRO010000480 DATCRO010000000
VERSION     DATCRO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522643"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VIN75_13110"
     CDS             <1..387
                     /locus_tag="VIN75_13110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975154.1"
                     /translation="AFRIMKDQKPMGGRIINNGSISAHAPRPDSAPYTATKHAVTGLT
                     KSISLDGRAYDIACGQIDIGNAVTDMAARMATGVKQADGRIAAEAMLDVEHVADAVVH
                     MAGLPLEANVQFMTIMATKMPFVGRG"
     gene            complement(421..795)
                     /locus_tag="VIN75_13115"
     CDS             complement(421..795)
                     /locus_tag="VIN75_13115"
                     /inference="COORDINATES: protein motif:HMM:NF040085.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HEY8975155.1"
                     /translation="MTRLSRIQAAIDIAVAHGIPRADAAPLFHRLMWRLGFGIRPPHF
                     EQFGRNAGLMGLVWGSCMTVFAVLYALHLHPGGGVAVIGKVVGVAVVAATVFGLAMAA
                     IVRARARKARLPAWDDVAAHSR"
     gene            1029..1247
                     /locus_tag="VIN75_13120"
     CDS             1029..1247
                     /locus_tag="VIN75_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975156.1"
                     /translation="MSSLSAKLHHWSDGVAHVFAPHPAHFDPESHRLNDNPLRARLRP
                     IHLLPLVIPGMAVLLAVCAVLIGSLMRG"
     gene            complement(1278..2594)
                     /locus_tag="VIN75_13125"
     CDS             complement(1278..2594)
                     /locus_tag="VIN75_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8975157.1"
                     /translation="MKVRTVLLLLLLVVVLLAAWILLPWWGALILAALLAAWMAATRA
                     GRRAASVAAVGISTLGQRIGSSAVVVVGIAGVVGVLVAMLAMAEGYAEMLRKTGSLDT
                     AIVLRGASAAEVASGLDRDSVLQIAQTPGIARDAKGEPLASPELVVAANLPIKDATGD
                     EEGSVQLRGVGDEAWAVRPQVHIVEGRKFSPGLRELIVGRGAARQFAGLVPGHTIRLG
                     SQTWTVAGVFASGDALESEIWADANVVAGTYERGSSRNSVTVRLADAKSFAPFKAALE
                     ANPQLKIDASTTLDFFGKQSEGMSQFIRIIGIVIGGIMAVGAMFGALNTMFAAVDARA
                     REIATLRAIGFAGLPVVVAILLETMLLALAGGVLGGALAWLLFNGYAASTMAAGTVGK
                     LSFELHVTAGVLWQGLKWALAIGFIGGLFPAVRAATMPVTTALREL"
     gene            complement(2605..3771)
                     /locus_tag="VIN75_13130"
     CDS             complement(2605..3771)
                     /locus_tag="VIN75_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8975158.1"
                     /translation="MKYLHLIWASLFRRKTRTILTLVSIVAAFLLFGLLDAVRTSFAE
                     AGQSANGAQRLQTGSRLSFIQLLPQSLQARMEQVPGVKTVTYANWFGGAYQDPHNQVF
                     SFAVAPNYLDVYPEMSVSEAERKSFADTRTGVLIGEGLAQRYHWKVGDRIPMQSTIFP
                     DKSGSKNWVFDVAGIIHVTDKKSAGFFDQLMLLHWNYFDDTTPYNRGGVGWYVTKVAD
                     VNQADRVAKAIDALSANSDHETRTQTEQAATASWMKQFANVGLIVTSVMGAVFFTLML
                     LSGNTMMQAVRERTNELAVLKTIGFSGTSLMVMVLAESVLLLVLGGVVGILLAEPLIP
                     VISKALRGMIAPSPVSLASWAEGIVLMLVIAALVGALPAWRAMRLNIVDALARR"
     gene            complement(3791..4477)
                     /locus_tag="VIN75_13135"
     CDS             complement(3791..4477)
                     /locus_tag="VIN75_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975159.1"
                     /translation="MTQLIEIRDVSKIYERGRQTVEVLHHIDLDVAQGQFLALMGPSG
                     SGKTTLLNLIGGLDSPTGGSITVGGQRIDNLGASALAKWRASQVGFVFQFYNLMPMLS
                     AQRNVELPLLLTKLSGAERRKRASIALELVGLADRASHKPTELSGGQQQRVSIARAIV
                     SDPTLLVCDEPTGDLDRQSAEDVLALLRALNRDHGKTILMVTHDPKAADYANQTLHLD
                     KGTLVEQVAA"
     gene            complement(4509..5741)
                     /locus_tag="VIN75_13140"
     CDS             complement(4509..5741)
                     /locus_tag="VIN75_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8975160.1"
                     /translation="MNELRIQRTERDDHGGPPRWLWIALATVAVVAFLAAGGWWAWSS
                     RPVTVQTATATAPAGGGGSAGAVLQATGYVTPRRRATVSTQITGTLTQVLIEEGDHVQ
                     KGQVLARLEDSALRAGLDAARANLASAQAQVASAQAQLAQAQSDERRQAELLASGMVT
                     KAAGDQARTAVATAVAQLDARRREADAAKAQLGQAQVNFDYTVVRAPFAGVITEKAAQ
                     VGEIVSPLSAGGGFTRTGVGTIVDMDSLEVEVDVAEAYIGEVQAAMPAEAVLDAYPDW
                     KIPAHVIAVIPAADRGKGTVKVRVGLDQKDARLVPDIGVRVSFLARKDAATPAAAQAP
                     RGALVPAAAVVQRDGHAAVFVVVDGKAALRAVTPATPDVGTMKSIPAGISAGDKVVLS
                     PPATLQDGVAVKVEDAAH"
     gene            complement(5891..6691)
                     /gene="hyi"
                     /locus_tag="VIN75_13145"
     CDS             complement(5891..6691)
                     /gene="hyi"
                     /locus_tag="VIN75_13145"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEY8975161.1"
                     /translation="MPRFAANVSMLFTEAPFLDRFELAARAGFAAVECQFPYQAPAAE
                     IRARLDDLGLSMVLHNLPAGDWAAGDRGLAGLPHRTGEFRAGVPRAIAYAHALGVKQL
                     NCLAGIVPAGIEADTVHRTLVDNLRFAARALAAEGLALLVEPINTFDIPGFAVNRTAP
                     CVALLDEVGEPNAFVQYDAYHAQRMEGELAGTLARFMPRIAHVQVADNPGRHEPGTGE
                     IRFEFLFAELDRLGYAGWVGCEYTPAGRTDAGLGWLPPGALAIRRAGC"
     gene            complement(6702..7202)
                     /gene="mobB"
                     /locus_tag="VIN75_13150"
     CDS             complement(6702..7202)
                     /gene="mobB"
                     /locus_tag="VIN75_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY8975162.1"
                     /translation="MKVFGIAGYSGMGKTTLLARLVPALVARGLRVSMIKHSHKDIEV
                     DRPGKDSYRLREAGCHEVLLLGSTRWALMHELRGADEPSLDYLLDRLQDVDLVLVEGF
                     KHGDFPKLEVWRAEAGKPTLSPGWPGIHAIASDDRVEAGDGVAVLPLADTRAIADFVL
                     AHAAVR"
     gene            7482..>7997
                     /gene="rdgC"
                     /locus_tag="VIN75_13155"
     CDS             7482..>7997
                     /gene="rdgC"
                     /locus_tag="VIN75_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEY8975163.1"
                     /translation="MFKNVIVYRIDQWERPSLAELEPRLDAARFVECGAAQQESLGWV
                     EPRGEKHGPLVESVAGQWILKLCIETKAVPGGAVKSQLESRLDKIEQDTGRRPKGKAA
                     KELKEEIVHELLPRAFPKRGNTPIWIDLQKNLVVIGAGSAKKADKIVTLLTELLAGGI
                     RLELVQTQLSAA"
BASE COUNT         1179 a         2850 c         2841 g         1127 t
ORIGIN      
        1 gccttccgca tcatgaagga ccagaagccg atgggcggcc gcatcatcaa caacggctcg
       61 atctccgcgc atgcgccgcg tcccgattcc gcgccgtaca ccgccaccaa gcacgcggtg
      121 acgggcctga cgaagtcgat ctcgctggac ggccgcgcct acgacatcgc ctgcggccag
      181 atcgacatcg gcaacgccgt caccgacatg gccgcccgca tggccaccgg cgtgaagcag
      241 gccgacggcc gcatcgcggc ggaggcgatg ctggacgtcg agcacgtggc cgacgccgtg
      301 gtgcacatgg ccggcctgcc gctcgaggcg aacgtgcagt tcatgacgat catggccacg
      361 aagatgccgt tcgtcgggcg gggctgatcc gcaccgcccc gccggcgcgc gtcaggcgcc
      421 tcagcggctg tgcgccgcca cgtcgtccca ggccggcagc cttgccttgc gcgcgcgggc
      481 gcgcacgatg gccgccatcg ccaggccgaa cacggtggct gcgacgacgg cgacgccgac
      541 gaccttgccg atgacggcca cgccgcctcc tggatgcagg tgcagggcgt acaggacggc
      601 gaacaccgtc atgcaactgc cccagaccag gcccatgagc ccggcgttgc ggccgaactg
      661 ctcgaagtgc ggcggccgga tgccgaagcc cagccgccac atcaggcgat ggaacagggg
      721 cgccgcgtcg gcgcgcggga tgccatgcgc gacggcgatg tcgatggcgg cctggatccg
      781 ggaaaggcgt gtcatggggt gtcctgagtc gggccgattg tcactcggcc gctgtccgcc
      841 gggcctgccc cacccgggtg gaggtccggc gatacccgcg ccgccgtcgc atgttttcgc
      901 attccgccct tgcgccgggc cgcgcgcggg atcacattgt ccaggagagt gcgccgccat
      961 gggcacggtg gcggcgcgcg gggccgcgcg ccccgcgacc gcggtgccgc accgcccagg
     1021 aggctcgcat gtcttccctg tccgccaagc tgcatcactg gagcgacggc gtcgcgcacg
     1081 tgttcgcgcc gcatcccgcg cacttcgacc cggagtcgca ccggctcaac gacaacccgc
     1141 tgcgggcgcg gctgcgcccg atccacctgc tgccgctggt gatcccgggg atggccgtgc
     1201 tgctggccgt gtgcgcggtg ctgatcggct cgctgatgcg aggctgaacc cggggcgcga
     1261 cgaacgcgcc cccgatctca gagttcgcgc agcgcggtcg tgacgggcat cgtcgcggcg
     1321 cgcaccgcgg ggaacaggcc gccgatgaag ccgatggcca gggcccactt caggccctgc
     1381 cacagcacgc ccgcggtcac gtgcagctcg aagctgagct tgccgaccgt gccggcggcc
     1441 atcgtggagg cggcgtagcc gttgaacagc agccaggcca gcgccccgcc gagcacgccg
     1501 ccggccagcg ccagcagcat cgtctcgagc aggatcgcca ccacgacggg cagcccggca
     1561 aagccgatcg cgcgcagcgt ggcgatctcg cgcgcccgcg cgtcgaccgc cgcgaacatc
     1621 gtgttgagcg cgccgaacat cgcgccgacg gccatgatgc cgccgatgac gatgccgatg
     1681 atgcggatga actgcgacat gccctcggac tgcttgccga agaagtccag cgtggtgctg
     1741 gcgtcgatct tgagctgcgg gttggcttcg agcgcggcct tgaagggcgc gaagcttttg
     1801 gcgtccgcca ggcgcaccgt gaccgaattg cggctgctgc cgcgttcgta ggtgccggcc
     1861 acgacgttgg catcggccca gatctcggac tcgagcgcgt ccccgctggc gaacacgccg
     1921 gccacggtcc acgtctggct gcccaggcgg atcgtgtggc cggggacgag accggcgaac
     1981 tggcgcgccg ccccgcggcc gacgatcagc tcgcgcaggc ccgggctgaa cttgcggccc
     2041 tcgacgatat gcacctgcgg acgcacggcc caggcttcat cgcccacgcc gcgcagctgc
     2101 acgctgcctt cctcgtcgcc ggtggcgtcc ttgatcggca ggttggccgc gacgaccagc
     2161 tccggcgagg ccagcggctc gcccttggcg tcgcgcgcga tgccgggcgt ctgcgcgatc
     2221 tgcagcacgc tgtcgcgatc caggccggac gccacttcag ccgccgaggc gccgcgcagc
     2281 acgatcgcgg tgtcgaggct gccggtcttg cgcagcatct cggcatagcc ctcggccatc
     2341 gccagcatcg ccaccagcac gccgacgacg ccggcgatgc ccacgacgac gaccgccgac
     2401 gacccgatgc gctggccaag ggtgctgatg cccacggcgg ccacggacgc ggcgcggcgt
     2461 ccggcgcgcg tcgcggccat ccaggcggcc agcagcgcgg cgaggatcag cgcgccccac
     2521 catggaagga ggatccaggc cgccagcagg acgaccacga gcagcagcaa caacaggacg
     2581 gtgcgaacct tcatggcaat tccttcagcg gcgcgcgagc gcgtcgacga tgttcaggcg
     2641 catggcgcgc cacgccggca gcgcgccgac gagcgccgcg atgaccagca tcagcacgat
     2701 gccctccgcc cagctggcca ggctcacggg cgagggcgcg atcatgccgc gcagggcctt
     2761 gctgatcacg gggatcagcg gctcggccag caggatgccg acgacgcccc ccagcaccag
     2821 cagcaacacc gattccgcga gcaccatcac catcaggctc gtgcccgaga agccgatcgt
     2881 cttgagcacc gccaattcgt tcgtgcgctc gcgcacggcc tgcatcatcg tgttgcccga
     2941 cagcagcatc agcgtgaaga acacggcgcc catgaccgac gtgacgatga ggccgacgtt
     3001 ggcgaactgc ttcatccagc tggcggtcgc ggcctgttcg gtctgcgtgc gcgtctcgtg
     3061 gtccgagttc gccgacagcg cgtcgatcgc cttggccacg cgatcggcct ggttcacgtc
     3121 ggccaccttc gtgacgtacc agccgacgcc gccgcggttg tagggcgtgg tgtcgtcgaa
     3181 gtagttccag tgcagcagca tcagctggtc gaagaagccg gcggacttct tgtccgtgac
     3241 gtggatgatg ccggcgacgt cgaacaccca gttcttgctg ccggacttgt ccgggaagat
     3301 cgtcgactgc atcgggatgc ggtcgccgac cttccagtgg taccgctgcg cgagcccttc
     3361 gccgatcagc acgccggtgc gcgtgtcggc gaaggacttg cgctcggcct cgctgacgga
     3421 catctccggg tacacgtcca ggtagttcgg cgcgaccgcg aagctgaaga cctggttgtg
     3481 cggatcctgg taggcaccgc cgaaccagtt ggcgtaggtg accgtcttga cgccgggcac
     3541 ctgctccatg cgggcctgca gcgactgcgg cagcagctgg atgaacgaca ggcgcgagcc
     3601 cgtctgcagg cgctgcgcgc cgttggcgct ctggcccgct tccgcgaacg aggtgcgcac
     3661 ggcatcgagc aggccgaaca gcaggaacgc cgcgacgatg gacacgagcg tgaggatcgt
     3721 gcgcgtcttg cggcggaaca gcgaggccca gatcaggtgc aggtatttca tgagcgactc
     3781 ccgcgccgcg tcaggccgcg acctgctcga ccagcgttcc cttgtccagg tgcagcgtct
     3841 ggttggcgta gtcggcggcc ttcgggtcgt gggtgaccat caggatggtc ttgccgtggt
     3901 cgcggttgag cgcgcgcagc agcgccagca cgtcctcggc cgactggcgg tcgaggtcgc
     3961 cggtgggctc gtcgcacacc agcagcgtcg ggtccgagac gatcgcgcgg gcgatcgaca
     4021 cgcgctgctg ctggccgccc gacagttcgg tcggcttgtg gctggcgcga tcggccagtc
     4081 ccaccagttc cagcgcgatg gacgcgcgct tgcggcgctc cgcgccggac agcttggtca
     4141 gcagcagcgg cagctcgaca ttgcgctggg ccgacagcat cggcatcagg ttgtagaact
     4201 ggaacacgaa gccgacctgc gacgcgcgcc acttggcgag cgcgctcgcc ccgaggttgt
     4261 cgatgcgctg gccgcccacc gtgatgctgc cgcccgtggg cgagtcgagg ccgccgatca
     4321 ggttcagcag cgtcgtcttg ccggagcccg agggccccat caaggcgagg aactggcctt
     4381 gggccacgtc gaggtcgatg tggtgcagca cctcgaccgt ctgcctgccg cgttcgtaga
     4441 tcttggagac gtcgcggatt tcgatcaact gggtcatctc gttctcgctc ctttgggact
     4501 cggaagaatc agtgggccgc gtcctcgacc ttcaccgcca cgccgtcctg cagcgtggcc
     4561 ggcggcgaca gcaccacctt gtcgccggcg gaaatgccgg cgggaatcga cttcatcgtg
     4621 ccgacgtccg gcgtcgcggg cgtgaccgcg cgcagcgccg ccttgccgtc cacgaccacg
     4681 aacaccgcgg cgtggccatc gcgctgcacg acggccgcgg ccggcaccag cgcgccgcgc
     4741 ggcgcctggg ccgccgcggg cgtcgccgca tccttcctgg cgaggaagga cacgcgcacg
     4801 ccgatgtcgg gcaccaggcg cgcgtccttc tggtcgaggc ccacgcgcac cttgaccgtg
     4861 cccttgccgc ggtcggcggc ggggatcacg gcgatcacgt gcgcggggat cttccagtcg
     4921 gggtaggcgt cgagcacggc ctcggccggc atggcggcct gcacctcgcc gatgtaggcc
     4981 tcggcgacgt cgacctcgac ctccagcgag tccatgtcga cgatggtgcc cacgccggtg
     5041 cgcgtgaagc cgccgccggc cgacagcggc gagacgatct cgccgacctg cgccgccttc
     5101 tcggtgatga cgcccgcaaa gggcgcacgc accaccgtgt agtcgaagtt gacctgcgcc
     5161 tgcccgagct gcgccttggc ggcgtcggcc tcgcggcggc gcgcgtcgag ctgggcgacg
     5221 gcggtggcca ccgcggtgcg cgcctggtcg ccggccgcct tggtgaccat gccgctcgcg
     5281 agcagctcgg cctggcggcg ctcgtccgat tgcgcctgcg cgagctgcgc ctgcgcggac
     5341 gcgacctgcg cctgcgcgct ggcgaggttg gcgcgcgcgg cgtcgaggcc ggcgcgcagc
     5401 gcgctgtcct cgaggcgggc cagcacctgg cccttctgca cgtggtcgcc ttcctcgatc
     5461 agcacctgcg tcagcgtgcc ggtgatctgc gtggagaccg tggcgcggcg ccgcggcgtc
     5521 acgtagcccg tggcctgcag caccgcgccg gccgaaccgc cgccgccggc gggcgccgtg
     5581 gcggtggccg tctgcaccgt gacgggacgc gagctccacg cccaccagcc gcccgcggcg
     5641 aggaacgcca cgacggcgac ggtcgccagc gcgatccaca gccagcgcgg cggcccgccg
     5701 tggtcatcgc gttcggtgcg ctggatgcgc agctcgttca agaggttcgc gtcgatcttg
     5761 aagtctccgg caagagggtc caggagcgtg ccgcggaccc gacggcaccg cgcactctaa
     5821 cagtcacgtt atcgaaactc cttgatcgat cctgcggtgg accgaccaat ggcggttcga
     5881 aggcggcgaa tcaacagccg gcgcgacgaa tggccagcgc gccgggcggc agccagccca
     5941 ggccggcgtc cgtccggccc gccggcgtgt actcgcagcc gacccagccg gcgtagccca
     6001 gccgatcgag ttcggcgaag aggaactcga agcggatctc gccggtgccc ggttcgtggc
     6061 ggcccgggtt gtcggcgacc tggacgtggg cgatgcgggg catgaagcgc gccagcgtgc
     6121 cggcgagctc gccttccatg cgctgcgcgt ggtacgcgtc gtactgcacg aacgcgttcg
     6181 gctcgcccac ctcgtcgagc agcgcgacgc acggcgcggt gcggttgacg gcgaagccgg
     6241 ggatgtcgaa ggtgttgatc ggctcgacca gcaatgccag tccttccgcg gcgagcgcgc
     6301 gtgccgcgaa gcgcaggttg tcgacgaggg tacggtgcac ggtatcggcc tcgatgccgg
     6361 ccggcacgat gccggccagg cagttcagct gcttgacgcc cagcgcgtgc gcgtacgcga
     6421 tcgcgcgcgg gacgcccgcg cggaactcgc ccgtgcgatg cggcagcccg gccagcccgc
     6481 gatcgcccgc ggcccagtcg ccggcgggca ggttgtgcag caccatcgac agccccaggt
     6541 cgtccagccg cgcgcggatc tccgcggccg gcgcctggta cgggaactgg cattcgacgg
     6601 cggcgaagcc ggcgcgcgcg gcgagctcga agcggtcgag gaacggcgcc tcggtgaaca
     6661 gcatcgagac gttggcggca aagcgcggca tggcgggccc ttcagcgcac ggcggcgtgc
     6721 gcgagcacga agtcggcgat ggcccgggtg tcggccagcg gcagcacggc gacgccgtcg
     6781 cccgcctcga cgcggtcgtc gctggcgatg gcgtggatgc cgggccagcc gggcgacagc
     6841 gtcggcttgc cggcctcggc gcgccagacc tccagcttgg ggaaatcgcc atgcttgaag
     6901 ccctcgacca gcacgaggtc gacgtcctgc aggcggtcga gcaggtagtc cagcgacggc
     6961 tcgtcggcgc cgcgcagctc gtgcatcagc gcccagcgcg tgctgccgag cagcagcacc
     7021 tcgtggcagc cggcctcgcg caggcggtac gaatccttgc cggggcggtc gacctcgatg
     7081 tccttgtggc tgtgcttgat catggagacg cgcagcccgc gcgcgaccag cgccggcacc
     7141 agccgcgcca gcagcgtcgt cttgcccatg cccgaatagc ccgcgatgcc gaacaccttc
     7201 atgcggccat tctcgccctg agcggacggg ctggccgcag gcggtttcgt agggcgggtc
     7261 agccgcaggc gtaacccgcc atgtcggcat gtcgtgacgc cggcgaacgg cgggttacgc
     7321 ctgcggccaa cccgccctac cgtgtccggc acgcgcgcgc ggccacgcgc gtgcggtttt
     7381 gcccggttcc gcgcgccgcg gaacgtttcg ttgctggacg gaaatacagg tcgcctagaa
     7441 tcgcgcgctc cgctgcactt ccgtccttcc ggcccgaatc catgttcaag aacgtcatcg
     7501 tctaccgcat cgatcaatgg gaacgcccga gcctggccga gctggagccg cgcctggacg
     7561 cggcgcgctt cgtcgagtgc ggcgcggcgc agcaggagtc cctcggctgg gtcgagcctc
     7621 gcggcgagaa gcacgggccg ctggtcgaga gcgtcgcggg ccagtggatc ctgaagctgt
     7681 gcatcgagac caaggccgtg cccggcggcg ccgtcaagtc gcagctcgag tcgcgcctgg
     7741 acaagatcga gcaggacacc ggccgccgcc ccaagggcaa ggccgccaag gagctcaagg
     7801 aggagatcgt gcacgagctg ctgccgcgcg cgttccccaa gcgcggcaac acgccgatct
     7861 ggatcgacct gcagaagaac ctggtcgtca tcggcgccgg cagcgccaag aaggccgaca
     7921 agatcgtcac gctgctgacc gagctgctgg ccggcggcat ccggctcgag ctcgtgcaga
     7981 cgcagctgtc ggccgcc
//
LOCUS       DATCRO010000481         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_275411, whole genome shotgun sequence.
ACCESSION   DATCRO010000481 DATCRO010000000
VERSION     DATCRO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275411"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1142)
                     /gene="argF"
                     /locus_tag="VIN75_13160"
     CDS             complement(210..1142)
                     /gene="argF"
                     /locus_tag="VIN75_13160"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8975164.1"
                     /translation="MKPGDTLMKHYLQFKDFRAEEYAYLFERTREIKKRFKNYEKYIP
                     LVDRTLAMIFEKASTRTRVSFEAGMYQMGGSVVNLTTGDSQLGRAEPIEDSARVISRM
                     VDIVMIRTFEQTKIERFAAHSRVPVINGLTNEFHPCQIMADLFTFIEYRGAIAGRTVA
                     WVGDGNNMANTWVQAADILGFTLHVSTPSGYELDFDVAGVKNRACVKLFDNPLDACRG
                     ADLVTTDVWTSMGYEAENERRLVDFEKWCVDAEMMAVARPDALFMHCLPAHRGEEVTA
                     EVIDGPQSVVWDEAENRMHVQKALMEYLLLGRIG"
     gene            complement(1139..>2007)
                     /locus_tag="VIN75_13165"
     CDS             complement(1139..>2007)
                     /locus_tag="VIN75_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8975165.1"
                     /translation="IARKYGHDKGIERPEIIVCERAFHGRSLATLSATGNPKIQAGFG
                     PLVEGFVRVPFNDAAAVRAVAQSSKSVVAVMLEPVQGEGGINPARNDYLRELRAICDE
                     HGWLLMLDEVQCGIARTGKWFAFQWAGIQPDVMSLAKGLGSGMPVGALVVGPRAKDVF
                     GPGNHGSTFGGNPLAMRAAIETLNVIESDGLMKNAEVVGEHLRAALRREIGALPGVVD
                     IRGEGLMIGVELGKPCGAILTRAAEAGLLVSVTADTVIRIVPPLIFTTADADEVVARL
                     APLVKAFLAEQA"
BASE COUNT          288 a          697 c          682 g          340 t
ORIGIN      
        1 gcgctgacga gggggctggc ctctcctcgg atcgcgcaga tccgtcgcca tcgatcgtcc
       61 cgccgatcgc gattcgagga caggcctgac cccggtgatc gtctcgcgat cggggtcagg
      121 ccggtccttc gtccgcgatc ccggtcatcg cttcgatgaa ggaggcggcg cggacttcgg
      181 agaggccaga cccctccggt cagcccccgt catccaatgc gacccagcag caggtactcc
      241 atgagcgcct tctgcacgtg catgcggttc tccgcctcgt cccacaccac cgattgcggg
      301 ccgtcgatga cctcggcggt cacctcctcg ccgcggtgcg cgggaaggca gtgcatgaac
      361 agcgcgtcgg gcctggcgac ggccatcatc tcggcgtcga cgcaccactt ctcgaagtcg
      421 accaggcggc gctcgttctc ggcctcgtag cccatgctcg tccacacgtc ggtggtgacg
      481 aggtcggcgc cgcggcacgc gtccagcggg ttgtcgaaca gcttgacgca ggcgcggttc
      541 ttcacgccgg ccacgtcgaa gtcgagctcg tagccgctgg gggtgctcac gtgcagcgtg
      601 aagccgagga tgtcggcggc ctgcacccac gtgttggcca tgttgttgcc gtcgcccacc
      661 caggccaccg tgcggcccgc gatcgcgccg cggtactcga tgaaggtgaa caggtcggcc
      721 atgatctggc acgggtggaa ctcgttggtc aggccgttga tcaccggcac gcgcgagtgc
      781 gccgcgaagc gctcgatctt ggtctgctcg aacgtgcgga tcatcacgat gtcgaccatg
      841 cggctgatga cgcgcgccga gtcctcgatc ggctcggcgc ggcccagctg gctgtcgccg
      901 gtggtcaggt tcaccaccga gccgcccatc tggtacatgc cggcctcgaa gctcacgcgg
      961 gtgcgcgtgg aggccttctc gaagatcatc gccagcgtgc ggtccaccag cgggatgtac
     1021 ttttcgtagt tcttgaagcg cttcttgatc tcgcgcgtgc gctcgaacag gtaggcgtac
     1081 tcctcggccc ggaagtcctt gaactgcagg tagtgcttca tgagcgtgtc gccgggtttc
     1141 atgcctgctc cgcgaggaag gccttgacca gcggcgccag cctggcgacc acttcgtcgg
     1201 cgtcggcggt ggtgaagatc agcggcggca cgatgcggat gaccgtgtcg gccgtgacgc
     1261 tgaccagcag gcccgcctcg gccgcgcgcg tgaggatcgc gccgcacggc ttgccgagtt
     1321 cgacgccgat catcaggccc tcgccgcgga tgtcgacgac gcccggcaag gcgccgatct
     1381 cgcgccggag cgccgcgcgc aggtgctcgc cgaccacctc ggcgttcttc atcaggccgt
     1441 cggactcgat gacgttgagc gtctcgatgg ccgcgcgcat cgccagcgga ttgccgccga
     1501 aggtgctgcc gtggttgccc gggccgaaca cgtccttggc gcgcgggccc accacgagcg
     1561 cgcccaccgg catgcccgag cccaggccct tggccagcga catgacgtcg ggctgaatgc
     1621 cggcccactg gaacgcgaac cacttgccgg tgcgcgcgat gccgcactgc acctcgtcga
     1681 gcatcagcag ccagccgtgc tcgtcgcaga tggcgcgcag ctcgcgcagg tagtcgttgc
     1741 gggccgggtt gatgccgcct tcgccctgca cgggctccag catcaccgcg acgacgctct
     1801 tgctcgattg cgccacggca cggacggccg cggcgtcgtt gaacggcacg cgcacgaagc
     1861 cctcgaccag cgggccgaag ccggcctgga tcttcgggtt gccggtggcc gacagcgtgg
     1921 ccagcgagcg gccgtggaag gcgcgctcgc agacgatgat ctcggggcgc tcgatgccct
     1981 tgtcatggcc gtacttgcgc gcgatct
//
LOCUS       DATCRO010000482         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_714068, whole genome shotgun sequence.
ACCESSION   DATCRO010000482 DATCRO010000000
VERSION     DATCRO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714068"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VIN75_13170"
     CDS             complement(<1..1853)
                     /locus_tag="VIN75_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY8975166.1"
                     /translation="MTQPSSDITTTPDPDTAPLRQYALTDNLAADSGAVFLTGTQALV
                     RLLLMQRRRDQVAGLNTAAFVSGYRGSPLGMVDQQLWKAKKFLADAKVEFLPAINEDL
                     AATACLGTQRVALDPKREVEGVTAMWYGKGPGVDRSGDALKHGNVYGSSPKGGVLVVA
                     GDDHGCVSSSMPHQSDLAMQAWSMPVLHPANVAEYLEFGLYGWALSRFSGAWVGFKAI
                     SEVVESGMTVDLDAIPLDFTLPVDFTPSQDLHVRAVDLPSLLLESRLAEKLAAVRAFA
                     KVNSVDKHIVASPSATLGIVTVGKAHYDFLEVLRRLELDPNALAAAGVRIYKVGLVFP
                     LEPTRMAEFAQGLEEILVIEEKAPVVERQLKELLYGLPDLQRPRIAGKTTPDGAPLLS
                     SLGELRPSRIMEVVAGWLARLNPALDRTHLVTDFTMPCLLHNAADAVKRQPYFCSGCP
                     HNTSTKVPEGSRALAGIGCHFMASWMERETSGLIQMGAEGVDWAAHSRFTKEKHVFQN
                     LGDGTYYHSGYLAIRQAIAAKATITYKILYNDAVAMTGGQPVDGKLSVPEIARQVEAE
                     GAKRVVIVSDDITPHRAHANLFPAGTTFHPREELDAVQRELREIEGVTILI"
     gene            2066..2539
                     /locus_tag="VIN75_13175"
     CDS             2066..2539
                     /locus_tag="VIN75_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8975167.1"
                     /translation="MTLPASIDRVARRIAAALQRDGRQSAQQLADTVGLSATPVWRRV
                     KELEASGVIRGYVALLDREKLGLSICVLAHVTLVRHSEGAVEQFEAMARSSPEIMECH
                     STTGEGDYLIKVVAPDMKAYDRFLQDKVFKTPGVANVRSSVVLREVKYETALPVD"
     gene            2624..3214
                     /locus_tag="VIN75_13180"
     CDS             2624..3214
                     /locus_tag="VIN75_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8975168.1"
                     /translation="MSDTSSPDPRPLGLAQLLAIQAHANRLANRRLQAALAGLSTSDL
                     HAKRISFFPTLIGTLNHLLAVDRYYIAALQGEADMEQQYRRFAPHDELAPYAAAQDAS
                     DLKLIAFCDKLDDTSANAVVRMDRGEGKSQSDLACLVLQHLFMHQTHHRGQVHAMLAG
                     TQVKPPQLDEFIMRADEPFRVMDMSALGWTEREVYG"
     gene            3211..3870
                     /locus_tag="VIN75_13185"
     CDS             3211..3870
                     /locus_tag="VIN75_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8975169.1"
                     /translation="MSEASALPESALELWRFAAREATTVHIPPDGCRDLVVIVPRHGA
                     PVCLVSVLSQASEMHAFAPGDRAMGVRLRPGAQIDERALLALARAREAWDDDVLLDAV
                     GAATRLDARVDEALACLREAPSLPLAQARLGVSARSLERLLSPRTGRGPLWWRGLARA
                     RRCARGLAGETPLAALAADHGYADQAHMTRDLQRWFGASPSRLRAMPTMLAALASSGF
                     S"
BASE COUNT          561 a         1326 c         1407 g          580 t
ORIGIN      
        1 tagatgagga tcgtgacgcc ctcgatctcg cgcagctcgc gctgcaccgc gtcgagctcc
       61 tcgcgcggat ggaaggtggt gccggccggg aacaggttgg cgtgcgcgcg gtgcggggtg
      121 atgtcgtcgc tgacgatcac cacgcgcttg gcgccctcgg cctcgacctg gcgcgcgatc
      181 tcgggcaccg acagcttgcc gtcgaccggc tggcccccgg tcatggccac cgcgtcgttg
      241 tagaggatct tgtacgtgat cgtcgccttg gcggcgatcg cctggcggat ggcgaggtac
      301 ccggagtggt agtacgtgcc gtcgcccagg ttctggaaga cgtgcttttc cttggtgaag
      361 cgcgagtgcg ccgcccagtc gacgccttcg gcgcccatct ggatcaggcc gctcgtctcg
      421 cgctccatcc agctggccat gaagtggcag ccgatgccgg ccagcgcgcg cgagccctcg
      481 ggcaccttcg tggacgtgtt gtgcgggcag ccggagcaga agtagggctg gcgcttcacg
      541 gcatcggccg cgttgtgcag caggcagggc atcgtgaagt ccgtgaccag gtgcgtgcgg
      601 tccagcgccg ggttcagtcg cgcgagccac ccggccacca cttccatgat gcgcgacggc
      661 cgcagttcgc ccagcgacga cagcagcggc gcgccgtcgg gcgtcgtctt gccggcgatg
      721 cgcgggcgct gcaggtccgg caggccgtac agcaattcct tcagctggcg ttcgacgacg
      781 ggcgccttct cctcgatgac gaggatttcc tcgaggcctt gcgcgaactc cgccatgcgc
      841 gtgggctcca gcgggaacac gaggcccacc ttgtagatgc gcacaccggc cgcggccagc
      901 gcgttcgggt cgagctccag gcggcgcagc acctccagga agtcgtagtg cgccttgccc
      961 accgtgacga tgcccagcgt ggcgctgggg ctcgcgacga tgtgcttgtc gaccgagttc
     1021 accttcgcga acgcgcgcac ggcggccagc ttctcggcca ggcgcgattc cagcaacagc
     1081 gacggcaggt cgacggcgcg cacgtgcagg tcctgcgacg gcgtgaagtc cacgggcagc
     1141 gtgaagtcca gcgggatggc gtcgaggtcg acggtcatgc cggactcgac gacttcggag
     1201 atcgccttga agcccaccca cgcgcccgag aagcgcgaca gcgcccagcc gtacaggccg
     1261 aactccaggt actcggcgac gttggccggg tgcagcacgg gcatcgacca ggcctgcatc
     1321 gcgaggtcgc tctggtgcgg catcgacgac gacacgcagc cgtggtcgtc gccggccacg
     1381 accagcacgc cacccttggg gctggagccg tagacgttgc cgtgcttgag cgcgtcgccc
     1441 gagcggtcga cgccggggcc cttgccgtac cacatggccg tcacgccctc gacctcgcgc
     1501 ttgggatcga gcgcgacgcg ctgcgtgccc aggcaggcgg tggcggccag gtcttcgttg
     1561 atcgcgggca ggaactcgac cttcgcgtcg gccaggaatt tcttggcctt ccacagctgc
     1621 tggtcgacca tgcccagcgg gctgccgcgg tagccggaga cgaaggcggc ggtgttcagc
     1681 ccggcaacct ggtcccggcg gcgctgcatc agcagcaggc gcacgagcgc ctgggtgccg
     1741 gtcaggaaga cggcgcccga gtcggccgcg aggttgtcgg tgagcgcgta ctggcgcagc
     1801 ggcgcggtat cggggtcggg cgtggtggtg atgtccgagg agggctgggt catggcgtct
     1861 ggctcgcgct ggcaaggcgc tccgcgaagc gcgggcggct ggaccggcca tgctagacct
     1921 cgacgccggc agcgatattg cgcaatgggc acttgcaggc gaaatatcga tatgaacatt
     1981 tcgcaggacg tcgtcgcggg aaatgaatat ctcgatcggt gcagattccg agggtaaacg
     2041 cgctgccccg gcgcacaatg cgcccatgac cctgcccgcc tcgatcgacc gcgtcgcccg
     2101 ccgcatcgcc gccgccctgc agcgcgacgg ccgccagagc gcccagcaac tggccgacac
     2161 cgtggggttg tcggccacgc ccgtgtggcg gcgcgtgaag gagctggagg ccagcggcgt
     2221 catccgcggc tacgtggcgc tgctcgatcg cgagaagctg ggactgtcga tctgcgtcct
     2281 cgcgcacgtg acgctggtgc gccacagcga gggagcggtc gagcagttcg aggcgatggc
     2341 caggtccagc cccgagatca tggaatgcca cagcacgacg ggcgagggcg actacctcat
     2401 caaggtggtc gcgcctgaca tgaaggccta cgaccgcttc ctgcaggaca aggtcttcaa
     2461 gacgccgggc gtcgccaacg tgcgctccag cgtggtgctg cgcgaggtca agtacgagac
     2521 ggcgctcccg gtggattgat ccccagaaat cgacgctttc gcgcgcgcca tttgccccgg
     2581 atcggcgcta cgctgatcac gacgacaccg gagatcccac gccatgtccg acacctcttc
     2641 ccccgacccg cgcccgctgg ggctggcgca gctcctggcc atccaggccc atgccaaccg
     2701 cctggccaac cgccgactcc aggccgcact ggcgggcctg tcgacgtccg acttgcatgc
     2761 caagcgcatc agcttcttcc ccacgctcat cggcacgctc aaccacctgc tggccgtcga
     2821 ccgctactac atcgcggcgc tgcaggggga ggccgacatg gagcagcagt accgccgctt
     2881 tgcgccgcac gacgagctcg cgccctacgc ggcggcgcag gatgccagcg acctgaagct
     2941 gatcgcgttc tgcgacaagc tcgacgacac gtcggccaac gccgtcgtgc gcatggatcg
     3001 cggcgagggc aagtcgcaga gcgacctcgc ctgcctcgtg ctgcagcacc tgttcatgca
     3061 ccagacgcac caccgcgggc aggtgcacgc gatgctggcc ggcacgcagg tcaagccgcc
     3121 gcagctggac gagttcatca tgcgggcgga cgagccgttt cgcgtgatgg acatgtcggc
     3181 gctgggctgg acggagcgcg aggtctacgg atgagcgagg cgtcggccct gcccgaatcc
     3241 gcgctggagc tgtggcggtt cgccgcgcgc gaggcgacga cggtgcacat tccgccggac
     3301 ggttgccgcg acctggtcgt gatcgtgccg cgccacgggg cgccggtgtg cctggtcagc
     3361 gtgctgtccc aagccagcga gatgcacgcg ttcgcgcccg gcgaccgggc gatgggcgtg
     3421 cggttgcggc cgggcgcgca gatcgacgag cgggcgctcc tggcgctggc ccgggcccgc
     3481 gaggcttggg acgacgacgt cctgctggac gccgtgggcg cggccacgcg cctggacgca
     3541 cgggtggacg aggcgctggc ctgcttgcgt gaggcgccgt cgctgccgct cgcgcaggcg
     3601 cggctgggcg tgtcggcgcg cagcctggag cggctgctgt cgccccggac gggccgcggc
     3661 ccgctgtggt ggcgcggcct cgcgcgggcg cgccggtgcg cccgcgggct ggcgggcgag
     3721 acgccgctgg ccgcgctggc ggccgaccac ggctacgccg accaggcgca catgacgcgc
     3781 gacctgcagc gctggttcgg cgcgtcgccg agccggctgc gggcgatgcc gacgatgctg
     3841 gcggcgctgg cgtcgagcgg gttcagctag tgcc
//
LOCUS       DATCRO010000483         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_984069, whole genome shotgun sequence.
ACCESSION   DATCRO010000483 DATCRO010000000
VERSION     DATCRO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984069"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VIN75_13190"
     CDS             <1..439
                     /locus_tag="VIN75_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY8975170.1"
                     /translation="YAVAGLEGHVAELLVDKAQLLTLTAPEMTVLVGGLRVLGANVGN
                     AAHGVFTRRPGTLSNDFFVNLLDMRTRWQKSSTEGVLEGVDRKTGEKRWTGTVADLVF
                     GSNSQLRAIAEVYGASDGQEKFVRDFVAAWTKVMNLDRYDLKN"
     gene            721..1602
                     /gene="yedA"
                     /locus_tag="VIN75_13195"
     CDS             721..1602
                     /gene="yedA"
                     /locus_tag="VIN75_13195"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEY8975171.1"
                     /translation="MPNASRSTLLAACLAIVYVVWGSTYLAIHFVVDGIPPLASSAIR
                     NLCAGPVLLAFLKWRGVAWPTWRQWRNGALIGFMMMGLGNGFVCLGETKVPSGLASLV
                     VAGVPLFAMLFAWGLFGARPRVLEWLGVVLGLVGMALLNLDLHAAANPGAIAVLFIAA
                     AAWACATVLQPRLDLPRGPMSAAVQMLGGGLSLVVMATLHGERFDPAAVPASSWWALA
                     YLVVFGSLVAYTAFVYVIGHARPALATSYAYVNPVVAVGLGALFVGETISAPILAGMA
                     VILIGVALVMAGARRRA"
     gene            complement(1603..2622)
                     /locus_tag="VIN75_13200"
     CDS             complement(1603..2622)
                     /locus_tag="VIN75_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975172.1"
                     /translation="MHPRFTKFAVAATLLAASVAAHALDKVVFATNWKAQAAHGGFYQ
                     AVADGTYKRYGLDVEIRQGGPQVNNRPLLPAGRIDFLMTGNLLHSFDNVKNGVPVIAV
                     AAMFQKDPQALMAHPDGGYARFEDLAKAPVAYIAKDAQFSWWQWLKADYGFKDESLRP
                     YNYNVGPFLADKKSLQQGYAVEEPITIAKEGGFKPAVFLLADHGYSTYSTTIEARTDT
                     VRNKPDLVKRFVEASIIGWNNYLYGDRKAADALIKADNPEMNDTLIAQSIELMKQMGI
                     VDSGDAQTLGIGAMKPERIKDFYDKMVRAGLYKPGDVDLSKVATTEFVNKGVGVDVRR
                     KLTGK"
     gene            2735..>3956
                     /locus_tag="VIN75_13205"
     CDS             2735..>3956
                     /locus_tag="VIN75_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8975173.1"
                     /translation="MTLPHRQDTRLTTLPNGVRVVTIHMPWLASASASVFVRTGSGHE
                     SARDAGISHVVEHMAFKGTTTRDAARINIDAERLGAEVNAHTDKDHTAYHLSGMARDA
                     AQFVRMLGDIVRRPTFPALELERERQVILQEFIEDEEDPMSTAFRQFDKLCYGAHPMA
                     QAVIGTRRNIERFTREELLAYVERQYSGRNVVVGVAGNVDHDAIVREAEAAFGDMPPG
                     APHVMDAPTWLGGVKAARLAGCSQSHVVLGYPIAAMKDDDPAASVAAALFGEGMSSPL
                     MAEIRERRGLVYYAACSADVMEACGQFVIEASTTPDKVDELLRETHRLLREHAASVGA
                     LDLERARNQIAVRHLRAQERPVRRVEDAALDLFVHGRVRSPEELRDRIEAVSADQVRE
                     AFARMLAAPPVLALA"
BASE COUNT          528 a         1357 c         1424 g          647 t
ORIGIN      
        1 ctacgcggtc gccgggctcg aagggcatgt tgccgaactg ctggtcgaca aggcgcagct
       61 gctgacgctc acggcgcccg agatgacggt gctggtcggc ggccttcggg tgctcggcgc
      121 caacgtcggc aacgccgcgc atggcgtgtt caccaggcgt ccgggcacgc tgtcgaacga
      181 cttcttcgtc aacctgctgg acatgcgcac gcgctggcag aagtcgtcga ccgagggcgt
      241 gctggaaggc gtcgaccgca agacgggcga gaagaggtgg acgggcaccg tcgccgatct
      301 ggtgttcggt tcgaactcgc agctgcgcgc gatcgcggag gtctacggcg cgagcgacgg
      361 gcaggagaag ttcgtgcgcg acttcgtcgc tgcctggacc aaggtgatga acctggatcg
      421 gtacgacttg aagaattgac gcgcgggtgc ccggcgcgga cggaggggtc tggcccctcc
      481 ttcatccgcg cggtggcgtg gccatcgaca cggtgccggc tcgcggatga aggcaagggc
      541 ctgaccccga tctcatgccc ggcgcttctt tccgcgatcg gtgtcaggcc ttcgcctcgt
      601 catcgcggta ggcggtgccg ctttcggcga gggcgtggcg cgacgtcgag gagaggccag
      661 acccctccat tggcccgcgg cgaacgaggg ccggcacgtt tctgttccac tcggcgatcc
      721 atgccgaacg cctcccgctc cacgctgctc gccgcctgcc tcgccatcgt ctacgtcgtc
      781 tggggctcca cgtacctcgc gatccacttc gtcgtcgacg gcatcccgcc gctggcgtcc
      841 tcggcgatcc gcaacctgtg cgccggcccc gtcctgcttg cattcctgaa gtggcgcggc
      901 gtcgcctggc ccacctggcg gcagtggcgc aacggcgcgc tgatcggctt catgatgatg
      961 ggcctgggca acggcttcgt gtgcctgggc gagaccaagg tgccctcggg cctcgcgtcg
     1021 ctggtggtcg ccggcgtgcc gctgttcgcg atgctgttcg cctggggcct gttcggcgcg
     1081 cggccgcgcg tcctcgagtg gctcggcgtc gtcctgggcc tggtgggcat ggcgctgctg
     1141 aatctcgacc tgcacgccgc cgcgaacccg ggcgccatcg cggtgctgtt catcgccgcc
     1201 gcggcgtggg cctgcgccac cgtgctgcag ccgcggctgg acctgcccag gggcccgatg
     1261 tcggccgccg tgcagatgct gggcggcggc ctgagcctgg tcgtgatggc cacgctgcac
     1321 ggcgagcgct tcgacccggc ggcggtgccg gcgtcgtcct ggtgggcgct ggcctacctg
     1381 gtcgtgttcg gctcgctggt ggcctacacg gcgttcgtct acgtcatcgg ccacgcgcgc
     1441 ccggcgctgg ccacgagcta cgcctacgtc aatccggtgg tggccgtggg cctgggcgcg
     1501 ctgttcgtcg gcgagacgat cagcgcgccg atcctggccg gcatggcggt gatcctgatc
     1561 ggggtcgcgc tggtgatggc gggcgcgcgg cgccgcgcct gatcacttgc ccgtgagctt
     1621 cctgcgcacg tccacgccca cgcccttgtt cacgaactcg gtggtggcga ccttggacag
     1681 gtcgacgtcg ccgggcttgt agaggccggc gcgcaccatc ttgtcgtaga agtccttgat
     1741 gcgctcgggc ttcatcgcgc cgatgcccag ggtctgcgcg tcgccggaat cgacgatgcc
     1801 catctgcttc atcagctcga tgctctgcgc gatcagggtg tcgttcattt cggggttgtc
     1861 ggccttgatg agcgcgtccg cggccttgcg gtcgccgtac aggtagttgt tccagccgat
     1921 gatggacgcc tcgacgaagc gcttgacgag gtcgggcttg ttcctgacgg tatcggtgcg
     1981 cgcctcgatg gtcgtcgagt acgtgctgta cccgtggtcg gccagcagga acacggcggg
     2041 cttgaagccg ccttccttgg cgatggtgat cggctcctcc acggcgtagc cctgctgcag
     2101 gctcttcttg tcggccagga acgggccgac gttgtagttg tacgggcgca gcgactcgtc
     2161 cttgaagccg tagtcggcct tgagccactg ccaccacgag aactgcgcgt ccttggcgat
     2221 gtaggccacc ggcgccttgg ccaggtcctc gaacctggcg tagccgccgt ccgggtgcgc
     2281 catgagcgcc tgcgggtcct tctggaacat cgcggcgacc gcgatgacgg gcacgccgtt
     2341 cttgacgttg tcgaagctgt gcagcaggtt gcccgtcatc aggaagtcga tgcggccggc
     2401 cggcagcagc gggcggttgt tgacctgcgg gccgccctgg cggatctcga cgtccaggcc
     2461 gtagcgcttg tacgtgccat cggccacggc ctggtagaag ccgccgtgcg cggcctgcgc
     2521 cttccagttg gtggcgaaga cgaccttgtc cagggcgtgg gcggcgacgc tggccgccag
     2581 caacgtggcg gcgacggcga atttcgtgaa gcgaggatgc atggacagct ccgggggtgc
     2641 tcaggcgacg ggcgatcata cgggcggccg tcgagccggg gaagacaagg ccccgcgcgc
     2701 ggcagcggcc gggcgcgtcg ccgacaatac gggcatgacc cttccgcacc gccaggacac
     2761 ccgcctcacc accctgccca acggcgtgcg cgtcgtcacg atccacatgc cgtggctcgc
     2821 gagcgccagc gccagcgtgt tcgtgcgcac cggctcgggc cacgagagcg cgcgcgacgc
     2881 gggcatcagc cacgtcgtcg agcacatggc gttcaagggc accaccacgc gcgacgctgc
     2941 gcgcatcaac atcgacgccg agcgcctggg cgccgaggtc aacgcgcaca ccgacaagga
     3001 ccacacggcc taccacctga gcggcatggc gcgcgacgcc gcgcagttcg tgcgcatgct
     3061 gggcgacatc gtgcgccggc ccacgttccc ggcgctcgag ctcgagcgcg agcgccaggt
     3121 gatcctgcag gagttcatcg aggacgagga agacccgatg tcgaccgcgt tccgccagtt
     3181 cgacaagctc tgctacggcg cgcacccgat ggcgcaggcc gtgatcggca cgcgccgcaa
     3241 catcgagcgc ttcacgcgcg aggagctgct ggcctacgtc gagcgccagt acagcggccg
     3301 gaacgtggtg gtgggcgtgg ccggcaacgt cgaccacgac gccatcgtgc gcgaggccga
     3361 ggccgcgttc ggcgacatgc cgcccggcgc gccgcacgtg atggacgcgc ccacgtggct
     3421 gggcggcgtc aaggccgcgc gcctggccgg ctgcagccag agccacgtgg tgctcggcta
     3481 cccgatcgcc gcgatgaagg acgacgatcc ggccgccagc gtggccgccg cgctgttcgg
     3541 cgagggcatg agctcgccgc tgatggccga gatccgcgag cgccgcggcc tcgtctacta
     3601 cgccgcgtgc tcggccgacg tcatggaggc ctgcggccag ttcgtgatcg aggcgtcgac
     3661 cacgcccgac aaggtcgacg agctgctgcg cgagacgcac cgcctgctgc gggagcacgc
     3721 cgcgtccgtg ggcgccctcg atctcgaacg cgcccgcaac cagatcgcgg tgcgccacct
     3781 gcgcgcgcag gagcggccgg tgcgccgcgt cgaggacgcc gcactcgacc tgttcgtgca
     3841 tggccgggtc cgctcgcccg aggaactgcg cgatcgcatc gaggcggtgt cggccgacca
     3901 ggtgcgcgag gccttcgcac gcatgctggc tgcgccgccg gtgctcgcgc tggcgg
//
LOCUS       DATCRO010000484        11771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_241292, whole genome shotgun sequence.
ACCESSION   DATCRO010000484 DATCRO010000000
VERSION     DATCRO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241292"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1268)
                     /locus_tag="VIN75_13210"
     CDS             complement(21..1268)
                     /locus_tag="VIN75_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975174.1"
                     /translation="MPDHTCAPDDDEPTAAQAAAHARALRGAAKLAVEATTGLTDLVE
                     AVHARIATLPGLPGPADGRTRGITGLVYKTIRGVTRAVGGSVDTLLPVLAPLLAAGGA
                     APALPSPEREAVLAALNGVLGDHLARTANPLAIRMALRREGRALALQRDALAAAMPEA
                     GERVLVLVHGLCMNDLQWRRDGHDHGAALADSLGFTPLYLHYASGLHVSTNGRALSNL
                     LEQLLSQWPRPIARFAIVGHSMGGLVARGALHYGRAAGQHWVDRLDDLACLGTPHHGA
                     PLERAGQGLHMLLDATPYAGPFGRLAKLRSAGITDLRHGNVVDEDWLDADRFDSATDR
                     RRPVPLPAGPRCFALAGTLSGAAKALGDGLVPVASALGKHRLARRTLKFAPSHQRVFD
                     DTNHMQLLSSPAVFEQLRDWLRH"
     gene            1543..2040
                     /locus_tag="VIN75_13215"
     CDS             1543..2040
                     /locus_tag="VIN75_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8975175.1"
                     /translation="MTQTNLFRNRPVAVIGAGVMGIGIVQVALLAGHPVHLHDVREGA
                     AAAAAEQLAATLAKLADKGKLTPEDVAAARGRLSVATSLHDLRDAALVIEAIVENLAV
                     KQALMRDLEAIVSHDCVLASNTSSLSITAVARGLELPGRVVGMHFFNPVPLMRLVEVV
                     SGLAT"
     gene            2047..3108
                     /locus_tag="VIN75_13220"
     CDS             2047..3108
                     /locus_tag="VIN75_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8975176.1"
                     /translation="MADAIAMLAADWGKTAVHTKSTPGFIVNRIARPFYAETLALLHE
                     QAATPAVLDACVRGAGFRMGPCELMDLIGHDTNFAVTQSVYEANFFDKRYQPSLVQRE
                     MVDGGLLGRKSGRGFYRYPEGGPRTVDGDAEAPALPVVPLVLRGEGPVADLLASRLAA
                     SGLGFTRETPAGDARMSVTLMVGDGVLQPTPGRTATQDAAVRGVPDVAVFDWPVGPAA
                     GPAATPVVPLAYAVAAQSSAAFAEQVALVLRALGWAPQRVKDTPGLVVARTISMLVNE
                     GSDAVDQGVCTPEGADAAMTLGVNYPAGPFAWLALWGPAPIVGILHELDQHYRGERYR
                     ASPRLQREFWASAGAPAQP"
     gene            complement(3133..5373)
                     /locus_tag="VIN75_13225"
     CDS             complement(3133..5373)
                     /locus_tag="VIN75_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975177.1"
                     /translation="MSQGRVRPMGWRRLLLLPIALLASLAVVLLARTIAQTPNLDASW
                     RLDGNGHLQLIATDLPWMRPALGHSLEVIVAPGIAPLVVDGRRVANSTRWIANDDERA
                     AAAQQQRQMARVLEAGTPVLLGFTDRMNARVVPHPRGYTTLGITFWLLCGLGLALELV
                     GAVVVLVRQDVQTLLYAIMAQCQAVQLLLAAVDAVPGMGNISLVGGNEAALRMSLDVV
                     TCAALLNATLLYPTPLPPRRLLSWLAWIAAIVFTTAAWAFDLPQAWWWCEALSLSTGL
                     LVVGLLGWSYRITPHPYATVMRRLSIVTTGSLALVTIVVALSDRMPGEVQSLVTAVPV
                     IWTVFFASLLLMSPFLARSQHMMREFAMLAGVSTLATSVDLLFVAVFSFNQFASITTS
                     LFVALGVYAASRQWLLDQMIGARAVTTERLFEHLLRIARKVEEQPERAADWQLELFHA
                     VFQPLVARHEPGFTRTTRWYEGGAGLLVPLAVEPQSDPSQLDEGDAPEARLIVLRFAE
                     RGKRLFTAEDARLADRIVEQLRRAVAHDRAIEQGRSEERGRIAQDLHDDIGARLLTLM
                     YKAPNAEMEDYVRHTLQDLKTLTRGLAATSHYLSHSAGEWKADIAQRLAPTGCTLEWS
                     FEQDAEIQLNVVQWSGLTRILRELVSNIIAHAKATHATIHGELRDGVLALTVTDNGVG
                     GTPKDWSTGLGLGGIRKRVRMLGGTIAWQQNDPEGVRCDVSVTLGAPSTPTVPSTSTS
                     PGAAAN"
     gene            complement(5581..6363)
                     /locus_tag="VIN75_13230"
     CDS             complement(5581..6363)
                     /locus_tag="VIN75_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975178.1"
                     /translation="MAVKVLILEDNPAAREFLGRVVRESFSDTITIVEAGDLESARRA
                     VRIAGGAQGYHGVDPFKLILVDLELPDGSGLELLEELRQYPATKIVTTLYSDDEHLFP
                     ALQCGADGYLLKEDRFELLVEELQKIVRGEPPLSPAIARRLLGHFRTHTRTAMNRRIT
                     DEGNGAALRAPAAAGTFTSSLAFAAPAPVEIEPPLDLPALTPREVEVLTFLSKGFTIK
                     EIAALMSIKWFTVNDHIKSIYRKLNVSSRAEAAVLASKQGLV"
     gene            complement(6530..7528)
                     /locus_tag="VIN75_13235"
     CDS             complement(6530..7528)
                     /locus_tag="VIN75_13235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY8975179.1"
                     /translation="MQAWMCETLDGPAALQWRERPTPTPAADEVLIAIHAASLNFPDL
                     LIVQGKYQMRPELPFAPGAEFAGIVQAVGSDVKHLRPGMPVAALGGHGGFATHACVKA
                     MQVMPLPPGFALRDAAAFAFTYGTAHHALVDRGALQAGETVLVLGAAGGVGTAAIQIA
                     KAAGAKVIAAASTDAKCELCREIGADVAINYATTPLKDTLKAATGGKGPDVVFDPVGG
                     SHAEPAFRAIAWRGRYLVIGFAEGRIPALPWNLPLLKGASIVGVFWGDFVRREPAANA
                     HMLGVLAAQYARGQVKPVIDDVLPLSRLADAYHRLENRQILGKLVLVPDDLLAAKA"
     gene            7628..8401
                     /gene="surE"
                     /locus_tag="VIN75_13240"
     CDS             7628..8401
                     /gene="surE"
                     /locus_tag="VIN75_13240"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY8975180.1"
                     /translation="MRILIANDDGYLAAGLVALVEACRGLGEIDVVAPEQNASGMSNS
                     LTLNRPLSVFVAKGGPSDGFRVVNGTPSDCVHVAMTGLLEHRPDLVLSGINNGENMGD
                     DTLYSGTVAAAMEGFLFGVPAIAFSLKHKGWGNLDAAARTARRVVQAAVADPSFGRPG
                     TAPWLLNVNIPNRPDADTLPWATTRLGRRHASEPVIAQKNPRGDTIYWIGPAGDAREA
                     GAGTDFHAVASGRVSITPLQVDLTDHASLAGWSSRLGGQ"
     gene            8398..9219
                     /locus_tag="VIN75_13245"
     CDS             8398..9219
                     /locus_tag="VIN75_13245"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8975181.1"
                     /translation="MSGDAPRAKRFPAQWTSGSRPAAPNGTARNPLRPQALLHQAAQD
                     AARHVVPTGLGLDNGPMRERMVARLRAEGIRTHKLLDAFAAVPRHRFVDTALAIQAYE
                     DTSLPIGLGQTISKPSVVARQLQWLFDGANASRLGHLGRTLEIGTGCGYQTALLARLA
                     PTVISIERLQPLHQMARRHLDALGVPGVRLVHGDGRVGHAPNAPYDSIVAAAGGEAVP
                     EAWLQQLAVGGRLVAPTAMPGLSGQALVIVDRTPDGFVRQLGESVQFVPLKSGIG"
     gene            9237..10160
                     /locus_tag="VIN75_13250"
     CDS             9237..10160
                     /locus_tag="VIN75_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8975182.1"
                     /translation="MASLGRRTLVTTVCVASAWALIGCATPNQAPVEDHSARTVRAPA
                     PAETAKPAAQTLPVAESRPGTYTVQPGDILTKIGMDTGQSWRDLARWNNLTDPNHLEV
                     GQVLRVVPPAGEVAPGSTVTTTTTTTTAAASAPATFKPLPPIPSKPEGKASGATAAST
                     AVASGSAVPLTGAATPQPAAARETDDDMTWAWPAAGSVISPFDDTKTKGLAIGGKAGD
                     PVYAAADGRVVYAGSGLRGYGNLVIVKHNANYLTAYAHNQTLLVKEDQIVKRGQKIAE
                     MGSTDADRVELHFEIRKQGKPIDPARLLPQR"
     gene            10480..10842
                     /locus_tag="VIN75_13255"
     CDS             10480..10842
                     /locus_tag="VIN75_13255"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8975183.1"
                     /translation="MTRIAHLTTIRGALIGTVCALASVAALAGDGKWVQDAKSCAAPE
                     YPRDSLAKGEQGTTGLRILVGVDGKAIDSEIETSSHSRKLDSAAQRALMECSYKPQPA
                     DSKPAQQWIKTEFVWKLN"
     gene            11003..>11771
                     /locus_tag="VIN75_13260"
     CDS             11003..>11771
                     /locus_tag="VIN75_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEY8975184.1"
                     /translation="MLTNLKIGARLALAFGIVLALLATLSIIALARTSAMRNDMNAVT
                     GGIAVESALADQLRVTATQQAVRVRNILMVSEPEEMAAENELLKADVKSYETAKAKLA
                     KMIAVENDARKKTILGHIDATEKEARDLADQVVQMALDNHKFPARGLLGDKANPAQAK
                     WMAAVDEMVALNEKLTAAAVASAESAYGNTRNIVLGISIIALIFASAVGYAVTRSITV
                     PISQAVGIARRVADRDLSVTVTSHSNDETGQLLKALAA"
BASE COUNT         1757 a         4187 c         4230 g         1597 t
ORIGIN      
        1 ccctacgaga tccgagacga tcagtgccgc agccaatcgc gcagttgttc gaacaccgcc
       61 gggctggaca gcagctgcat gtggttggtg tcatcgaaga cgcgctggtg cgacggcgcg
      121 aacttgagcg tgcgccgcgc gagccgatgc ttgccgaggg cgctcgccac cggcacgagg
      181 ccgtcgccga gcgccttggc cgccccggac agcgtgcccg ccagcgcgaa gcagcgcggg
      241 cccgcgggca ggggcacggg ccggcggcgg tcggtggcgc tgtcgaagcg gtcggcatcg
      301 agccagtcct cgtcgacgac gttgccgtgc cgcaggtcgg tgatgccggc gctgcgcagc
      361 ttggcgaggc ggccgaacgg gccggcgtag ggcgtggcgt ccagcagcat gtgcaggccc
      421 tggcccgcgc gttccagcgg cgcgccgtgg tgcggcgtgc ccaggcaggc gaggtcgtcc
      481 agccggtcga cccagtgctg gccggccgcg cggccgtagt gcagcgcgcc gcgcgcgacg
      541 aggccgccca tgctgtggcc gacgatcgcg aagcgcgcga tcggccgcgg ccattgcgac
      601 agcaactgct cgagcaggtt cgacagcgcg cgcccgttgg tcgacacgtg caggccgctc
      661 gcgtagtgca ggtacagcgg cgtgaagccc agcgagtcgg ccagggccgc gccgtggtcg
      721 tggccgtcgc gccgccattg caggtcgttc atgcacaggc cgtggacgag caccagcacg
      781 cgctcgccgg cctcgggcat cgcggcggcc agggcgtcgc gctgcaacgc gagcgcgcgg
      841 ccctcgcgcc gcagcgccat gcggatcgcg agcgggttgg ccgtgcgcgc aaggtggtcg
      901 cccagcacgc cgttgagcgc ggcgagcacg gcctcgcgct cgggcgacgg cagcgccggg
      961 gccgcgccac cggcggcgag cagcggcgcc agcacgggca gcagcgtgtc gacgctgccg
     1021 ccgaccgcgc gcgtcacgcc gcggatcgtc ttgtagacca ggcccgtgat gccgcgcgtg
     1081 cggccgtccg cggggccggg caagcccggc agcgtggcga tgcgcgcatg cacggcctcg
     1141 acgaggtcgg tcagtcccgt ggtcgcctcg acggccagct tcgcggcgcc gcgcagggcg
     1201 cgcgcgtgcg cggccgcctg cgcggcggtg ggctcgtcgt cgtcgggggc gcaagtgtgg
     1261 tcgggcatgg gcacgtcaag ggcggcgcga atgagcgccc gccccgagtt tgccagtgcg
     1321 ggatgacgcg accggttgct aaccgatgaa tgcgtcggaa agagaaggtc cgcccgtcgg
     1381 atcggcgtca cggccacagg gaggcttggt cgttcgtttc gacccgacac cgcgtgaaac
     1441 gcggccacgc gggcgggaaa gggccgcgag atcgcgacgt gcgaattgtc gaagtcgacc
     1501 ccttccgcag cctgaagcca gccataacgg aaaatggcgg acatgacgca aaccaacctg
     1561 ttccgcaatc gtcccgtcgc cgtcatcggc gccggcgtga tgggcatcgg catcgtgcag
     1621 gtcgcgctgc tggccgggca ccccgtgcac ctgcacgacg tgcgcgaggg cgcggccgcg
     1681 gcggccgccg agcagctcgc cgccacgctg gccaagctgg ccgacaaggg caagctgacg
     1741 ccggaggacg tcgccgccgc gcgcggccgg ctgtccgtcg cgacttcgct gcacgacctg
     1801 cgcgacgccg cgctcgtgat cgaggcgatc gtggagaacc tggccgtcaa gcaggcgctg
     1861 atgcgcgacc tcgaggccat cgtgtcgcac gactgcgtgc tggcgtccaa cacgtcgtcg
     1921 ctgtcgatca ccgcggtcgc gcgcgggctg gagctgccgg gtcgcgtggt gggcatgcat
     1981 ttcttcaacc cggtgccgct gatgcgcctg gtggaggtcg tctcgggcct ggccacctaa
     2041 ccggccgtgg ccgacgccat cgcgatgctg gccgccgact ggggcaagac cgcggtgcac
     2101 acgaagtcga cccccggctt catcgtcaac cgcatcgcgc gcccgttcta cgccgagacg
     2161 ctggcgctgc tgcatgaaca ggccgcgacg cccgcggtgc tcgacgcctg cgtgcgcggc
     2221 gcgggcttcc gcatggggcc gtgcgagctg atggacctga tcggccacga caccaacttc
     2281 gccgtcaccc aatcggtcta cgaggccaac ttcttcgaca agcgctacca gccctcgctg
     2341 gtgcagcgcg agatggtcga cggcggcctg ctggggcgca agtcgggccg cggcttctac
     2401 cgctacccgg aaggcggacc caggacggtc gatggcgatg ccgaggcccc ggcactgccc
     2461 gtggttccgc tggtgctgcg cggcgagggg ccggtggccg acctgctcgc atcgcgcctg
     2521 gcggcctcgg gcctgggctt cacgcgcgag accccggcgg gcgatgccag gatgtccgtc
     2581 acgctgatgg tcggcgacgg cgtgctgcag cccacgcccg ggcgaacggc cacgcaggac
     2641 gccgccgttc gcggcgtgcc ggacgtggcc gtgttcgact ggccggtcgg cccggccgcg
     2701 ggccccgcgg cgaccccggt cgtgccgctg gcctacgcgg tcgcggcgca gtcgtcggcg
     2761 gcgttcgccg agcaggtggc gctcgtgctg cgtgcgctcg gctgggcgcc gcaacgcgtc
     2821 aaggacacgc cgggcctcgt cgtcgcgcgc acgatctcga tgctggtcaa cgaaggcagc
     2881 gacgccgtcg accagggcgt gtgcacgccc gagggcgcgg acgccgcgat gacgctgggc
     2941 gtcaactacc cggcgggtcc gttcgcctgg ctggcgctgt gggggccggc gcccatcgtc
     3001 gggatcctgc acgagctcga ccagcactac cgcggcgaac gctaccgagc cagcccgcgg
     3061 ctgcagcgcg agttctgggc gtcggccggc gcgcccgcgc agccctgaac gccgccaccg
     3121 cggccgccgg gctcagttcg ccgccgcgcc gggcgaggtg gaggtcgacg gaacggtcgg
     3181 cgtcgacggc gcccccagcg tcacgctgac gtcgcagcgc acgccctcgg gatcgttctg
     3241 ctgccaggcg atggtgccgc ccagcatgcg cacgcgcttg cggatgccgc ccaggccgag
     3301 gcccgtcgac cagtccttgg gcgtgccgcc cacgccgttg tcggtgacgg tcagcgcgag
     3361 cacgccgtcg cgcaactcgc cgtggatcgt ggcgtgcgtg gccttggcgt gggcgatgat
     3421 gttgctcacc agctcgcgca ggatgcgcgt caggccggac cactgcacga cgttcagctg
     3481 gatctcggcg tcctgctcga acgaccactc cagcgtgcag ccggtgggcg cgaggcgctg
     3541 tgcgatgtcc gccttccact cgccggccga gtgcgacagg tagtggctgg tggccgcgag
     3601 gccgcgcgtg agcgtcttga ggtcctgcag cgtgtggcgg acgtagtcct ccatctccgc
     3661 gttgggcgcc ttgtacatga gcgtgagcag gcgcgcgccg atgtcgtcgt gcaggtcctg
     3721 cgcgatgcgg ccgcgctcct cgctgcggcc ctgctcgatg gcgcggtcgt gcgcgacggc
     3781 gcggcgcagc tgttcgacga tgcgatcggc caggcgcgca tcctcggccg tgaacagtcg
     3841 cttgccgcgc tcggcaaagc gcagcacgat gagccgcgcc tcgggtgcgt cgccttcgtc
     3901 cagctgcgac gggtccgact gcggctcgac cgccagcggc accagcaggc cggcgccgcc
     3961 ctcgtaccag cgcgtggtgc gcgtgaagcc gggctcgtgg cgcgccacca gcggctggaa
     4021 caccgcatgg aacagctcca gctgccagtc ggcggcgcgt tcgggctgtt cctccacctt
     4081 gcgtgcgatg cgcagcaggt gctcgaacag gcgctcggtg gtgacggcgc gcgcgccgat
     4141 catctggtcc agcagccact ggcgcgaggc cgcgtacacg ccgagagcga cgaacagcga
     4201 ggtggtgatc gacgcgaact ggttgaacga gaacaccgcg acgaacagca ggtcgaccga
     4261 cgtcgccagc gtgctgacgc cggccagcat cgcgaactcg cgcatcatgt gctgcgagcg
     4321 ggcgaggaac ggcgacatca gcagcagcga cgcgaagaac accgtccaga tcaccggcac
     4381 cgcggtcacc agcgactgca cctcgccggg catgcggtcg ctgagcgcga cgacgatcgt
     4441 gaccagcgcg aggctgcccg tggtgacgat gctcagccgc cgcatcaccg tggcgtacgg
     4501 atgcggcgtg atgcggtacg accaccccag caggccgacc accagcaggc cggtgctcag
     4561 cgacagcgcc tcgcaccacc accaggcctg cggcaggtcg aacgcccacg ccgcggtggt
     4621 gaagacgatg gcggcgatcc acgccagcca cgacagcagc cgccgcggcg gcagcggggt
     4681 gggatacagc agcgtcgcgt tgagcagggc ggcgcaggtg acgacgtcca gcgacatgcg
     4741 cagcgccgcc tcgttgccgc ccaccagcga gatgttgccc atgccgggca ccgcgtcgac
     4801 cgcggccagc agcagctgca ccgcctggca ctgcgccatg atcgcgtaca ggagggtctg
     4861 cacgtcctgg cgcaccagca cgaccaccgc gcccaccagc tccagcgcca ggcccaggcc
     4921 gcacagcagc cagaaggtga tgcccagcgt ggtgtagccg cgcgggtgcg gcaccacgcg
     4981 ggcgttcatg cggtcggtga acccgagcag caccggggtg ccggcctcga ggacgcgcgc
     5041 catctggcgc tgctgctgcg ccgcggcggc gcgttcgtcg tcgttggcga tccagcgcgt
     5101 ggaattggcg acgcgccggc cgtcgaccac cagcggcgcg atgccggggg cgacgatgac
     5161 ctccagcgaa tgacccagcg cggggcgcat ccacggcagg tcggtggcga tcagctggag
     5221 atggccgttg ccgtccaggc gccagctggc gtccaggttg ggcgtctgcg cgatggtgcg
     5281 cgcgagcagc accaccgcga gcgaggccag cagcgcgatc ggcagcagca gcagccggcg
     5341 ccagcccatc gggcgcacgc ggccttggct catcgggacg ggaccggtga gtgcgcggcg
     5401 cgcgcgtccg ctggcacgtt cagaacctgc gaatggattc ctcgagtccg agcgggtcgt
     5461 ccaaaggcgt ccgatctagg cgcaaaggcg agtcgtgctg gtggcacggc gagcgtttgc
     5521 aacgacgagc gggcgtcttt gggcggcacg agcgggcgtg tgggattcgt tcacgggttc
     5581 ttaaactagg ccctgcttgc tggccagcac cgcggcttcg gcgcggctgg acacgttgag
     5641 cttgcggtag atcgacttga tgtggtcgtt caccgtgaac cacttgatcg acatcagggc
     5701 cgcgatctcc ttgatcgtga agcccttgct caggaaggtg aggacctcca cctcgcgcgg
     5761 cgtcagcgcc ggaaggtcca gcggcggctc gatctccacc ggcgccggcg cggcgaacgc
     5821 gagcgacgac gtgaaggtgc cggcggcggc gggcgcgcgc agtgcagcgc cgttgccctc
     5881 gtcggtgatg cggcggttca tggccgttcg ggtatgcgta cggaagtgcc ccagcagccg
     5941 gcgcgcgatg gccggcgaca gcggcggctc gccgcgcacg atcttctgca gttcctcgac
     6001 cagcagctcg aagcggtctt ccttgagcag gtagccgtcg gcgccgcatt gcagggccgg
     6061 gaacaggtgt tcgtcgtccg agtagagcgt ggtgacgatc ttggtggccg ggtactgacg
     6121 cagttcctcc agcagctcca gtcccgagcc gtccggcagc tcgaggtcca ccaggatcag
     6181 cttgaacgga tcgacgccgt gatacccctg ggcgccgccg gcgatgcgga ccgcgcgccg
     6241 tgcgctctcc agatcgcccg cctcgacgat ggtgatggtg tcgctgaagc tctcgcgcac
     6301 cacgcgtccg aggaattccc gcgccgcggg gttgtcctcg aggatcaaaa ccttgactgc
     6361 catgtcgttc ccctttgcct gcctgtgtcg tcgcctgacg gaatggactt ccgttcgcat
     6421 ggcgagcgat cccggaatgc taacgaggca aggggcccgt cgcatccccc gtttcggggc
     6481 agccgaatgg ggggaactgc ctattggggc tggccccgca gggtcttgct caggccttgg
     6541 ctgccaacag gtcgtccggc accagtacca gcttgcccag gatctgccgg ttctcgaggc
     6601 gatggtacgc gtccgccagc cgcgagaggg gcaatacgtc gtcgatcacc ggcttgacct
     6661 gcccgcgggc gtattgtgcc gccaacacgc ccagcatgtg cgcgttggcg gccggctcgc
     6721 ggcgcacgaa atcgccccag aacacgccca cgatggacgc gcccttgagc agcggcaggt
     6781 tccagggcag cgccgggatc cggccctcgg cgaagccgat cacgaggtag cgcccgcgcc
     6841 aggcgatggc gcggaacgcc ggctccgcgt gggagccgcc caccgggtcg aagacgacgt
     6901 ccgggccctt gccgcccgtg gcggccttga gcgtgtcctt cagcggggtg gtggcgtagt
     6961 tgatcgcgac gtcggcgccg atctcgcggc acagctcgca cttggcgtcg gtggacgccg
     7021 cggcgatcac cttggcgccg gcggccttgg cgatctggat ggccgccgtg ccgacgccgc
     7081 cggcggcgcc cagcaccagc accgtctcgc cggcctgcag cgcgccgcgg tcgaccagcg
     7141 cgtggtgcgc cgtgccgtag gtgaaggcga acgccgcggc gtcgcgcagc gcgaagccgg
     7201 gcggcagagg catcacctgc atcgccttga cgcaggcgtg ggtggcgaag ccgccatggc
     7261 cgccgagggc cgcgaccggc atgccggggc gcaggtgctt cacgtcgctg cccacggcct
     7321 ggacgatgcc cgcgaactcc gcgcccgggg cgaacggcag ctcgggccgc atctggtact
     7381 tgccctgcac gatcagcagg tcggggaagt tcagcgaggc cgcgtggatg gcgatcagca
     7441 cctcgtcggc tgcgggcgtc ggggtggggc gctcgcgcca ttgcagggcc gcggggccgt
     7501 ccagggtctc acacatccag gcttgcatga cagcgctttc cggttctgaa gttccttcga
     7561 tgatacggat ggcgcggcgc cgcggcagtc gccggggcga cgctgtcgcg ctgcctacaa
     7621 tgagcccatg cgcatcctca tcgccaacga cgacggctac ctggctgcgg gcctcgtggc
     7681 actggtcgaa gcgtgccgcg gcctgggcga gatcgacgtc gtcgcccccg agcagaacgc
     7741 cagcggcatg tccaactcgc tgacgctgaa ccggccgctg tcggtgttcg tggccaaggg
     7801 cggcccgtcc gacgggtttc gcgtcgtcaa cggcacgccg tccgactgcg tgcacgtggc
     7861 catgacgggc ctgctcgaac accggcccga cctggtgctc tcgggcatca acaacggcga
     7921 gaacatgggc gacgacacgc tctattcggg caccgtcgcc gcggcgatgg agggcttcct
     7981 gttcggcgtg ccggcgatcg cgttctcgct caagcacaag ggctggggca acctggacgc
     8041 cgcagcgcgc accgcgcgcc gcgtggtgca ggcggccgtc gccgacccgt cgttcggccg
     8101 gcccggcacc gcgccctggc tgctgaacgt caacatcccg aaccggcccg acgccgacac
     8161 gctgccctgg gccaccacgc gcctgggccg gcgccacgcc agcgagccgg tcatcgcgca
     8221 gaagaacccg cgcggcgaca cgatctactg gatcggcccc gcgggcgacg cgcgcgaggc
     8281 cggcgccggc acggacttcc acgccgtggc gagcggccgc gtgtcgatca cgccgctgca
     8341 ggtcgacctc acggaccacg cgagcctcgc gggctggtcg tcgcgactgg gcgggcaatg
     8401 agcggggacg cgccgcgcgc caagcgtttc ccggcccagt ggacgagcgg ctcgcgcccc
     8461 gcggccccca acggcaccgc gcgcaacccg ctgcggccac aggcgctgct gcaccaggcg
     8521 gcgcaggacg ccgcgcgcca cgtggtgccc accggcctgg gcctggacaa cgggccgatg
     8581 cgcgagcgca tggtcgcgcg cctgcgcgcc gagggcatcc gcacgcacaa gctgctcgat
     8641 gcgttcgccg cggtgccgcg ccaccgcttc gtcgacaccg cgctggccat ccaggcgtac
     8701 gaagacacca gcctgccgat cggcctgggc cagaccatct ccaagccgtc ggtcgtcgcg
     8761 cgccagctgc agtggctctt cgacggcgcc aacgcgagcc gcctgggcca cctcgggcgc
     8821 acgctggaga tcggcacggg ctgcggctac cagacggccc tgctggcgcg cctggcgccg
     8881 accgtgatct cgatcgagcg cctgcagccg ctgcaccaga tggcgcggcg ccacctggac
     8941 gcgctgggcg tgcccggcgt gcgcctggtc cacggcgacg ggcgcgtggg ccacgcgccc
     9001 aacgcgccgt acgacagcat cgtggccgca gccggcggcg aggcggtgcc cgaggcctgg
     9061 ctgcagcagc tggccgtcgg cggccggctg gtcgcgccga ccgccatgcc ggggctgtcc
     9121 gggcaggcgc tggtcatcgt cgaccggacc ccggacgggt tcgtgcggca gctcggcgag
     9181 tcggtccaat ttgttccctt aaaatcaggg attggatgaa acaacgcagg gaaactatgg
     9241 catcgctggg acgacgaacg ctcgtgacga cggtttgcgt ggcgtccgcc tgggccttga
     9301 tcggctgcgc gacccccaac caggcacccg tggaagacca ctcggcccgg acggtccggg
     9361 cgcccgcgcc cgccgagacg gccaagcccg cggcccagac gctgcctgtg gccgagtcgc
     9421 ggccgggcac ctacaccgtc cagccgggcg acatcctgac caagatcggc atggacaccg
     9481 gccagagctg gcgtgacctg gcgcgctgga acaacctgac cgacccgaac cacctcgagg
     9541 tcgggcaggt gctgcgcgtc gtgccgccgg ccggcgaggt cgcgcccggc tcgaccgtga
     9601 cgaccacgac gacgaccacc acggccgccg cgtccgcccc ggccaccttc aagccgctgc
     9661 cgccgatccc gtcaaagccc gagggcaagg ccagcggcgc gacggccgca tcgacggcgg
     9721 tcgcctccgg cagcgccgtg ccgctgacgg gcgccgccac gccgcagccg gccgccgcgc
     9781 gcgagaccga cgacgacatg acctgggcct ggccggcggc cggttcggtg atctcgccgt
     9841 tcgacgacac caagaccaag ggcctggcca tcggcggcaa ggccggcgac ccggtctacg
     9901 cggccgccga cggccgtgtc gtctacgccg gctccggcct gcgcggctac ggcaacctgg
     9961 tcatcgtcaa gcacaacgcg aactacctga ccgcctacgc ccacaaccag acgctgctgg
    10021 tcaaggaaga ccagatcgtc aagcgcggcc agaagatcgc cgagatgggc tcgaccgacg
    10081 ccgatcgggt ggaactgcac ttcgagatcc gcaagcaggg caagccgatc gatccggcgc
    10141 gcctgctgcc gcagcgctga acggcgctcc cgcacccccg tgaaaacgcg cccccgaggc
    10201 gcgttttttt tggcccgcga gggtcgatga cggtcacgac ccgaccgcgc aagcgctttc
    10261 gcacacggcg cccgttttgg tcggggagcc ccccgcgagt ccgtgatctg ggctgttttt
    10321 cccgcattga tacccggttc agcgtgaagg attcactcaa gaatgatcgt gcggggccga
    10381 aatcgggcca tgacccctgt caggcgggtg ccactccggt gggacaaccc tgtcaggacg
    10441 acacaagatc catttcgacc cgctgaagga gttcacagaa tgacccgcat tgcacacctg
    10501 accacgatcc gtggcgccct gatcggcacc gtctgcgcgc tcgcgagcgt tgccgcgctg
    10561 gccggtgacg gcaagtgggt gcaagacgcc aagagctgcg ccgcgcccga gtacccgcgc
    10621 gattcgctgg ccaagggcga gcagggcacg accggcctgc gaatcctggt gggcgtggac
    10681 ggcaaggcca tcgacagcga gatcgagacc tccagccatt cccgcaagct cgactcggcc
    10741 gcgcagcgcg cgctgatgga gtgctcgtac aagccgcagc ccgccgactc caagccggcc
    10801 cagcagtgga tcaagaccga gttcgtctgg aagctgaact gaccttttct ttccctccgg
    10861 cgacgacgcc gggccgcccg acgcgaggtc tggaacaccg gacggcgggc cgcgataccg
    10921 ccgggatggt cgcttttgtc cgttgggggg cagggccggc ctgcaagacc cacaagatca
    10981 acagccaact tcggataacc aaatgctgac caacctgaaa atcggcgcgc gattggccct
    11041 cgcgttcggc atcgtgctgg ccctgctggc gaccctgtcg atcatcgccc tggcgcgcac
    11101 cagcgcgatg cgcaacgaca tgaacgccgt gacgggcggc atcgccgtgg aatccgcgct
    11161 ggccgaccag ctgcgcgtca cggccaccca gcaggcggtg cgcgtgcgca acatcctgat
    11221 ggtgtccgaa cccgaggaga tggccgccga gaacgagctg ctcaaggccg acgtcaagag
    11281 ctacgagacg gccaaggcca agctcgccaa gatgatcgcg gtcgagaacg acgcccgcaa
    11341 gaagaccatc ctgggccaca tcgacgccac cgagaaggaa gcgcgcgacc tggccgacca
    11401 ggtggtgcag atggccctcg acaaccacaa gttccccgcc cgcggcctgc tgggcgacaa
    11461 ggccaacccg gcgcaagcca agtggatggc cgccgtcgac gagatggtcg ccctcaacga
    11521 gaagctgacc gccgccgcgg tcgccagcgc cgagagcgcc tacggcaaca cccgcaacat
    11581 cgtgctgggc atctcgatca tcgcgctgat cttcgcgtcg gccgtcggct acgccgtcac
    11641 ccgctcgatc acggtgccga tctcgcaggc cgtgggcatc gcccgccgcg tggccgatcg
    11701 cgacctcagc gtcacggtga cgtcccattc gaacgacgag acaggccagc tgctcaaggc
    11761 gctggccgcg a
//
LOCUS       DATCRO010000485         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_95050, whole genome shotgun sequence.
ACCESSION   DATCRO010000485 DATCRO010000000
VERSION     DATCRO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95050"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>3288
                     /locus_tag="VIN75_13265"
     CDS             29..>3288
                     /locus_tag="VIN75_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8975185.1"
                     /translation="MAVDAVTLEAPPLAEGWPWPDAARDECGGVAWVAPQGPVEEMLA
                     SAWADVLGEPRVGRFDDFRAADGFSLLVVQVAARLNPHFASESLARELHASRAIWHLA
                     RRLEPTHVPRQAREPRPQPQRAAHARGAEPFALTPYQDALWQAQRIAAGTGLYNIALA
                     WSLRGAVDVPALAAALRTLVQRHAALRCVFDADTGAQHVRQAPAFELDVADVADTDDA
                     VAEAVRAEAVAPFDLAAGAFRARLLRRAPGRHVLLMTVHHLVADGWSVGLLTRQLGIE
                     YAARLAGTATDALVPPAREYADFAHDVASRPSVDAAVQHWRAALAGPLPALELPFDRP
                     RAAAGAARSDVVDFQWTSALEDGLRNAAQTLDCTLFELLAAAMQVWIARSTGTRDVRL
                     AYPVANRAQPEFHGVVGCFVNTLVLCTHVDAAAPARALVADVQRALRAADHHQHLPLE
                     RLVEIAAERSESGAALWPVALNFNLADRPALELARLEVDALPRVSAGSPFALSWIVSR
                     AQGRLGGRLEFDAARFDRATVAAMPRQLGALLQSIADGVDTPVGRLSMLDEDDRLALL
                     RLAGDPAPPGAGPFVPVQAAFEARCRATPSAPALRVDGQTIAYARLNERANRLAHLLR
                     AHGAAAESVVAVCLPRGADMIVALLAILKAGAAWLPLDKDQPPRRLALMRAEAGARLA
                     VTVESLRPLLRAHPRDHVLCLDTAHASLAAQPAADLALAVHPEQLAYCLFTSGSTGTP
                     KGVMISARALAGHVEASIARYGVHAQDRLLQFAAVTFDTSIEQMLVPLCAGACMVVRG
                     ETPWSPDELAQLLRDEAVTVADIPTAYWSQYAQADVDPLADSALRLLLIGGEGALASA
                     AGAHRRRFPTFNAYGPTEATVTACVAEVGAGDAGIGPYVPIGRPAAGTRVHVLDDALQ
                     PVPRGVAGDIHIAGERLARGYLLRPAMTAERFVPDPFGAPGARLYRTGDRGRVMADGR
                     LEFLGRADDQVKIRGIRIELGEVENTLRAHPAVREVAVVVGGDTADDVFLSAHVVLDA
                     ADAADAVRLHARTALPAAMVPSQWHFPHALPLTPHGKVDRKRLARMQPSRAQAVPTA"
BASE COUNT          374 a         1308 c         1166 g          440 t
ORIGIN      
        1 gcgcgccgcg tgcggcgcgg acgtcgcgat ggccgtcgac gccgtcacgc tcgaggcgcc
       61 gccactggcc gaggggtggc cgtggccgga cgccgcgcgc gacgaatgcg gcggcgtcgc
      121 ctgggtcgcg ccacaaggcc cggtcgagga aatgctcgcc agcgcctggg ccgacgtgct
      181 cggcgaaccg cgcgtcggcc gcttcgacga cttccgtgcc gccgacggct tctcgctgct
      241 cgtcgtgcag gtcgcggcac ggctgaatcc gcacttcgcc agcgagtcgc tggcccgcga
      301 actccacgcc tctcgcgcga tctggcacct ggcccgacgc ctcgagccga cgcacgtccc
      361 ccgccaggct cgggagccgc gaccgcagcc gcagcgggcc gcgcacgcgc gcggggccga
      421 gcccttcgcg ctgacgccct accaggacgc cctgtggcag gcgcagcgca tcgcagccgg
      481 cacgggcctg tacaacatcg cgctggcgtg gtccctccgc ggcgcggtcg acgtccccgc
      541 gctcgccgcc gcgctgcgca cgctggtgca acgccacgcc gccctgcgct gcgtcttcga
      601 tgcggacacc ggcgcgcagc acgtgcgcca ggcgccggcg ttcgagctcg acgtcgccga
      661 tgtcgccgac accgacgacg ccgtggccga ggccgtccgc gccgaagcag tcgcgccgtt
      721 cgacctcgcg gccggcgcct tccgtgcccg gttgctgcgg cgtgcacccg gccggcacgt
      781 cctgctgatg accgtccacc acctcgtggc ggatggctgg tccgtgggcc tgctcacccg
      841 ccagctcggc atcgagtacg ccgcgcgcct cgccgggacc gcgacggacg cactcgtgcc
      901 gccggcccgt gagtacgccg atttcgcgca cgacgtcgcc tcgcgcccgt ccgtcgacgc
      961 cgccgtgcag cattggcgcg cggcgctggc cggcccgctg cccgcgctcg agttgccttt
     1021 cgaccggcca cgtgcggccg caggcgcggc acgcagcgac gtcgtggact tccaatggac
     1081 gtccgccctc gaggacggcc tgcgcaacgc ggcgcagacc ctcgactgca cgctgttcga
     1141 gttgctcgcc gcggccatgc aggtctggat cgcccgctcg accggcacgc gcgacgtccg
     1201 cctggcctat ccggtggcca atcgtgcgca gcccgagttc cacggcgtcg tcggctgctt
     1261 cgtcaacacc ctcgtcctgt gcacgcacgt cgacgccgcc gcccctgcgc gggcgctcgt
     1321 cgccgacgtg cagcgcgcgc tgcgtgccgc cgaccaccac cagcacctgc cgctggagcg
     1381 actggtcgag atcgccgcgg aacgaagcga atcgggcgcc gcgctgtggc cggtcgcgtt
     1441 gaacttcaat ctggccgacc ggcctgcgct cgagctcgcg aggctcgagg tcgacgcgct
     1501 gccgcgcgtg tccgccggat cgccgttcgc gctgagctgg atcgtgtcgc gcgcgcaggg
     1561 ccgcctcggc gggcgcctcg agttcgacgc cgcgcgcttc gatcgcgcca ccgtcgccgc
     1621 gatgccgcgt cagctcggcg cgctgctgca atcgatcgcg gacggcgtcg acacgcctgt
     1681 cgggcggctg tccatgctgg acgaggacga tcgcctcgcc ctgctgcggc tcgccggcga
     1741 cccggccccg ccaggcgccg ggcccttcgt gccggtgcag gcggccttcg aggcacgctg
     1801 ccgcgcgaca ccctcggccc cggcattgcg ggtcgacggc cagaccatcg cctacgcgcg
     1861 gctgaacgag cgcgcgaacc gcctggccca cctgctgcgc gcgcatggcg ccgcggccga
     1921 gtcggtcgtg gcggtctgcc tgccgcgcgg cgccgacatg atcgtggcgc tgctcgcgat
     1981 cctcaaggcc ggcgcggcgt ggctcccgct cgacaaggac cagcccccgc ggcgcctcgc
     2041 gttgatgcgc gccgaagccg gcgcccgcct ggcggtgacg gtcgaatcgc tgcggcccct
     2101 gctgcgcgcc catccgcgcg accacgtcct gtgcctggac accgcgcacg ccagcctggc
     2161 cgcgcagccg gccgcggacc tcgcgcttgc cgtccacccc gagcaactgg cctactgcct
     2221 gttcacctcc ggctccaccg gcacgcccaa gggggtgatg atctccgcgc gggcgcttgc
     2281 aggccacgtc gaggccagca tcgcgcgcta cggcgtgcac gcgcaggacc gcctgctgca
     2341 gttcgccgca gtcaccttcg acacgtcgat cgagcagatg ctggtgccgc tgtgcgccgg
     2401 cgcctgcatg gtggtccgcg gcgagacgcc ctggtcgccg gacgagctcg cgcagctgct
     2461 gcgcgacgag gcggtgacgg tcgcggacat ccccacggcc tactggagcc agtacgccca
     2521 ggccgacgtc gacccgctcg ccgacagcgc gttgcgcctg ctcctgatcg gcggcgaagg
     2581 cgcgctcgcg tcggccgcgg gggcgcatcg ccgccgcttc ccgaccttca acgcctatgg
     2641 cccgaccgaa gccacggtga ccgcctgcgt ggccgaggtc ggcgccggcg acgcgggcat
     2701 cgggccgtac gtgcccatcg gccgtccggc ggcgggcacg cgggtccatg tgctggacga
     2761 cgcgttgcag ccggtgccgc gcggcgtcgc gggcgacatc cacatcgccg gcgagcgcct
     2821 ggcgcgtggc tacctcctgc gtccggccat gacggccgag cgcttcgtgc ccgatccctt
     2881 cggcgcgccc ggcgcgcgcc tgtaccgcac cggcgatcgc ggccgcgtga tggccgacgg
     2941 ccgcctcgag ttcctgggac gcgcggacga ccaggtcaag atccgcggca tccgcatcga
     3001 gctgggcgag gtcgagaaca cgctgcgcgc gcaccccgcg gtgcgcgagg tcgcggtggt
     3061 ggtcggcggc gatacggccg acgatgtctt cctgtccgcc cacgtggtgc tcgacgccgc
     3121 cgacgcggcc gacgccgtgc gcctgcacgc gcgcacggcg ctgccggcgg ccatggtgcc
     3181 gtcgcaatgg catttcccgc acgcgctgcc gctgacgccg catggcaagg tcgaccgcaa
     3241 gcggctcgcg cggatgcagc cgtcccgcgc gcaagccgtt cccaccgc
//
LOCUS       DATCRO010000486         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_162615, whole genome shotgun sequence.
ACCESSION   DATCRO010000486 DATCRO010000000
VERSION     DATCRO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162615"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIN75_13270"
     CDS             complement(<1..260)
                     /locus_tag="VIN75_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8975186.1"
                     /translation="MSHPLPRVIAHLDMDAFYASVELLRYPMLRGQAAVVGGGRRHTP
                     IEQADGTLRFFKLEDYAGRGVVTTSTYEARAFGVFSGMGMMH"
     gene            591..1493
                     /locus_tag="VIN75_13275"
     CDS             591..1493
                     /locus_tag="VIN75_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY8975187.1"
                     /translation="MTSPEEHQALLAASRALLLPLARLLVARGVPYAQAEESLKTAMV
                     QAAQEAHPDGLPHRLVSRIATITGINRREVTRLTRAEGAPHAEPQRSAASQTFSRWRA
                     NPDYLDAQGKPLTLRRQGDAPSFESLARGVTQDVHPRSLLDELLRLGMARLDTDDDTV
                     TLIHDAFVPSTDRVRMLEFLAHNVGDHLSAAVANVVGPAPRHLERAVFADGLSPRAVA
                     AAQAWVAEAWHKMAADLAPFIEKLIAEEADEPDSARQQRFRAGLYAYTERDDGAPEGA
                     GAPAAGADAGPRTPPATPGRRPTE"
     gene            1504..2793
                     /locus_tag="VIN75_13280"
     CDS             1504..2793
                     /locus_tag="VIN75_13280"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY8975188.1"
                     /translation="MTHAVQFPRPWRALSALLAAMALVVGCGGGADSGVGTGGTGSPQ
                     SFSQGPISGFGSIIVNGVHFDDSAASVVDDDGNALSNSKDLHLGTVVDVDGGVVTNNA
                     ATATTIRVHVDLVGPVTAAYDGTTGRLAVLGQPVRVVSTTALDGIAGGAAAITAGTTV
                     RISSLYDLATGVYVATRIDPAPGATHLAIRGAPSAIDTAAHTFTIGGAVFSYGNVATP
                     TPFAAGQLLRAVLAMAPDGQGRWVVTSFGQAQAVLPDGRAGGVDGVVSSVADATHFVV
                     NGVSVDATNAKVTPTGATVGVQSRVVVQGTVTGGVLVATSVHVRAAHDNDDDQGGLAA
                     GAGNGNNGNGNGNGNGNGNGNGNGNDRVEIDGPILTLDTARQTFTMRGPTTVSYATAS
                     FAGGGKVSGLAVGVVIDMSGNLSADGTYVIADKIKFH"
     gene            complement(2780..3181)
                     /locus_tag="VIN75_13285"
     CDS             complement(2780..3181)
                     /locus_tag="VIN75_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEY8975189.1"
                     /translation="MKLLLPWLAIVATMIALDVAWIGVLARPLYSRGIGHLMAEQPNF
                     VAALAFYLLYAAGLMAFVVLRQPAGDWKAAAAWGAAFGLIAYMTYDLTNLATLRDWPL
                     ALSFVDVAWGCVATGLAATAGRLVADRVSGT"
     gene            3318..3989
                     /locus_tag="VIN75_13290"
     CDS             3318..3989
                     /locus_tag="VIN75_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY8975190.1"
                     /translation="MTPPMKRFLHVFKVRPRLFIVIAIGVLTAWLLPMHVASQSVTRW
                     LVAWNVGAWLYVFLAAAMMIRSSREHMRHRAQLQDDGKIAILVLTAIAGVASLVAIGG
                     ELAVVKDLHGWARGLHIALAGTTVLSSWGFIQIMFTLHYAHDYYQAACHGHKPGLQFP
                     DDPDPDYGDFFYFAAVIGTSGQTADVSFVTKPMRRIGSMHCILAYLFNTTVLALLINI
                     GASLF"
     gene            4090..4470
                     /locus_tag="VIN75_13295"
     CDS             4090..4470
                     /locus_tag="VIN75_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975191.1"
                     /translation="MSAALGGAALAAEPPAASSDSPEADASLYGQSVTLPRQAAACSE
                     RIADYMPRFQKIYDAWRTDNAARIEAGGRFIHQQAVKGGVNADEGMAKLADADVERLR
                     KTSLELLARHCQLMLDSLAAPAAN"
     gene            4593..6017
                     /locus_tag="VIN75_13300"
     CDS             4593..6017
                     /locus_tag="VIN75_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975192.1"
                     /translation="MPLDEIMAMVGVAVQATLALLLLVVWRSLRARWTLLLSAGFIAV
                     TAQYFCVALGHYTRGASWQEPPKTLNAALALLAHGLITWALIDYVGLPPAWSRRLRQA
                     TVLVFGLALIALAAGMLSRGATIGVIGTFMCGWAALFGWALRREPRAGHGIVMLAVLV
                     YPVSMLAALEGWFSTELAGISLIVPHAVLGMTILTTGLVRAQASSTRELAARQAVQAE
                     LERTNQTLELRVALRTAQLRETIEGLESFNRSVSHDLRGPLGGITGVARMAREALGQG
                     DLTAADKLLAAIARQSESSTDLVTSLLQLARSGEGPVERRTVHTDALVRELADLLAGA
                     AAATRQVVAIEGELPDLQADPALVRQVFANLLANGIKFSASASQPRVDVGATRGPGGH
                     TVFHVRDNGVGFTEAQGRELFVPFRRLHADRFEGHGIGLTIVRRIVERHGGRVWAESR
                     AGEGATFFFSFEEQTSDAKRREVA"
     gene            complement(6278..>6541)
                     /locus_tag="VIN75_13305"
     CDS             complement(6278..>6541)
                     /locus_tag="VIN75_13305"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795075.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY8975193.1"
                     /translation="ADYLVRKGVPFRDAHEVVAHAVKVGIDSGKDLAELPLDVLQGFH
                     ASIGQDVYGVLTLRGSLNARQVTGGTAPVEAQRQIARHKARLG"
BASE COUNT          927 a         2410 c         2292 g          912 t
ORIGIN      
        1 tgatgcatca tgcccatgcc cgagaagacg ccgaacgcgc gcgcctcgta cgtcgacgtg
       61 gtgacgacgc cgcggccggc gtagtcctcc agcttgaaga acctgagcgt gccatccgcc
      121 tgttcgatcg gcgtgtggcg ccggccgccg cccaccacgg ccgcctgtcc gcgcagcatc
      181 gggtagcgca gcagttcgac ggaggcgtag aacgcgtcca tgtcgaggtg cgcgatcacg
      241 cggggcagcg ggtggctcat gccggcattc tgtatgaaat cacaggtagc gggtggcccg
      301 gcgcgcggct tgcccggagt ttcgcgctgc aaatgtgaca acggcccatc tcggagatgc
      361 catgaacctg ccgcccatcc gccccccgcg caacgcatcg gactggatcg cgctcgcctc
      421 gacgctggcc gtcgcactgc tcgtgctggc cgcctgagcg cgccgtccgt gcgccgcggt
      481 gcgctggtcg ggtgaactcg accgttgggg gagcccccgg gcatcacccc acgattgttc
      541 ctgcgtgaat gggcgacact cccatctcaa cgtcctgtcc gctccccacc gtgacctcgc
      601 ccgaagaaca ccaggccctg ctcgccgcgt cccgcgccct gctgctgccg ctcgcccgcc
      661 tgctggtcgc gcgcggcgtg ccttatgcgc aagccgagga gagcctgaag accgcgatgg
      721 tgcaggcggc gcaggaggcg caccccgacg ggctgccgca ccgcctggtc agccgcatcg
      781 ccaccatcac cggcatcaac cggcgcgagg tgacgcgcct gacgcgcgcc gagggcgcgc
      841 cgcacgccga gccgcagcgc tcggcggcgt cgcagacctt ctcgcgctgg cgtgccaacc
      901 cggactacct cgatgcgcag ggcaagccgc tgacgctgcg ccgccagggc gacgcgccca
      961 gcttcgaatc gctggcgcgc ggcgtcacgc aggacgtgca tccgcgcagc ctgctcgacg
     1021 aactgctgcg cctgggcatg gcgcgcctgg acaccgacga cgacacggtg acgctgatcc
     1081 acgacgcctt cgtgcccagc accgaccgcg tccgcatgct cgaattcctg gcgcacaacg
     1141 tgggcgacca cctgtcggcc gccgtcgcca acgtggtggg ccccgcgccg cgccatctcg
     1201 aacgcgccgt gttcgccgac ggcctgtcgc ctcgcgccgt ggccgcggcc caggcctggg
     1261 tggccgaggc gtggcacaag atggccgcgg acctcgcgcc cttcatcgag aagctgatcg
     1321 ccgaggaggc ggacgaaccc gacagcgccc gccagcagcg tttccgcgcc ggcctgtatg
     1381 cgtacaccga gcgcgacgac ggcgcgcccg agggggccgg cgcacccgcc gcgggtgccg
     1441 acgccggccc gcgcacgccg cctgccacgc ccgggcgccg ccccaccgaa tgacgagacg
     1501 accatgaccc acgccgtgca attcccacgc ccctggcgcg cgctgagcgc acttcttgcc
     1561 gccatggcgc tcgtcgtcgg ctgcggcggc ggcgccgatt ccggcgtcgg caccggcggc
     1621 accggctcgc cccaaagctt ctcgcaaggc ccgatcagcg gcttcggctc gatcatcgtc
     1681 aacggcgtcc atttcgacga cagcgccgcc agcgtcgtgg acgacgacgg caacgcgctg
     1741 tcgaactcca aggacctgca cctgggcacg gtcgtcgacg tcgatggcgg cgtcgtcacg
     1801 aacaacgcgg cgaccgccac gacgatccgg gtgcacgtcg acctggtcgg cccggtcacc
     1861 gcggcatacg acggcaccac ggggcgcctg gccgtcctcg gccagccggt gcgcgtggtg
     1921 tccaccaccg cgctggacgg catcgccggt ggcgcggccg cgatcacggc gggcacgacg
     1981 gtcaggatct cgtcgctgta cgacctggcc accggcgtct acgtcgccac ccgcatcgat
     2041 ccggcgccgg gcgccacgca cttggcgatc cgcggcgcgc cgtcggccat cgacaccgcc
     2101 gcccacacct tcacgatcgg cggcgccgtc ttcagctacg gcaatgtcgc cacgcccacg
     2161 ccgttcgccg ccggccagct gctgcgcgcc gtgctggcga tggcgcccga cggccagggc
     2221 cgctgggtcg tcacctcgtt cggccaggcg caggccgtgc tgcccgacgg ccgcgcgggc
     2281 ggcgtcgacg gcgtggtcag cagcgtcgcc gacgccacgc acttcgtcgt caacggcgtg
     2341 tcggtcgacg cgaccaacgc caaggtcacg cccaccggcg cgaccgtcgg cgtgcagtcg
     2401 cgcgtcgtcg tgcaaggcac ggtcaccggc ggcgtgctgg tggccacgtc ggtgcatgtc
     2461 cgggcggcgc acgacaacga cgacgaccag ggcggcctgg ccgcgggggc cggcaacggc
     2521 aacaacggca atggcaatgg caatggcaat ggcaatggca acgggaacgg gaacggcaac
     2581 gaccgcgtcg agatcgacgg cccgatcctg acgctggaca cggcgcgcca gacgttcacg
     2641 atgcgcggac ccaccaccgt cagctatgcg acggcatcgt tcgcgggcgg aggaaaggtg
     2701 tccggcctcg cggtcggcgt cgtcatcgac atgagcggca acctgtcggc ggacggcacg
     2761 tacgtgatcg ccgacaagat caagttccac tgacccggtc ggccaccagc cggccggccg
     2821 tcgccgccag gccggtggcg acgcagcccc aggcgacgtc gacgaacgac agcgccagcg
     2881 gccagtcgcg cagcgtcgcc aggttggtca ggtcgtaggt catgtaggcg atgaggccga
     2941 aggcagcgcc ccaggcggct gccgccttcc agtcgcccgc cggctggcgc agcacgacga
     3001 aggccatcag gccggccgcg tacagcaggt agaacgcgag cgccgcgacg aagttcggct
     3061 gctcggccat caggtggccg atgccgcggc tgtacagcgg gcgagcgagc acgccgatcc
     3121 atgcgacgtc cagcgcgatc atggtggcga cgatggccag ccagggcaac agcagcttca
     3181 tgcggaactc cggtggacgg gcggccatcg tcgcacgaag gcgcaagcag cgcttcagct
     3241 tgcggggcgt cccgccgcgc ggcgctggac aatgcgggcg tcgcgcccgg cggcgcgcct
     3301 cttgccaagg ccgatccatg acccctccga tgaagcgctt cctgcacgtg ttcaaggtgc
     3361 ggccgcgcct gttcatcgtc atcgccatcg gggtgctcac cgcctggctg ctgccgatgc
     3421 acgtggcatc gcagtcggtc acgcgctggc tggtggcatg gaacgtcggc gcgtggctgt
     3481 acgtgttcct ggccgcggcg atgatgatcc gctcgtcgcg cgagcacatg cgccaccgcg
     3541 cgcagctgca ggacgacggc aagatcgcga tcctcgtgct gaccgccatc gccggcgtcg
     3601 cgagcctggt cgcgatcggc ggggagctgg cggtggtcaa ggacctgcac ggctgggcgc
     3661 gcgggctgca catcgccctg gcgggcacga cggtgctgtc gtcgtggggc ttcatccaga
     3721 tcatgttcac gctgcactac gcgcacgact actaccaggc ggcgtgccac ggccacaagc
     3781 cggggctgca gtttcccgac gacccggatc ccgactacgg cgacttcttc tatttcgccg
     3841 cggtgatcgg cacctcgggc cagacggccg acgtgtcctt cgtcaccaag cccatgcgcc
     3901 gcatcggctc gatgcactgc atcctggcct acctgttcaa cacgacagtg ctggcgctgc
     3961 tgatcaacat cggggcgagc ctgttctgac cgggatttcc cgggcacgga aggattccga
     4021 cacagtgtgt ccggccgacc ccggcacaat ggagggatgt cgttgcgaat ccacctcctt
     4081 gtgctcgccg tgtccgccgc cctcggcggc gcggcgcttg cggccgaacc gccggccgcg
     4141 tcgtcggaca gtcccgaggc cgacgcctcg ctgtacggcc agtcggtgac gctcccgcgc
     4201 caggccgccg cctgctcgga gcgcatcgcc gactacatgc cccgcttcca gaagatctac
     4261 gacgcctggc gcacggacaa cgcggcgcgc atcgaggccg gcggcaggtt catccaccag
     4321 caggcggtca agggcggcgt caatgccgac gaaggcatgg ccaagctggc ggacgcggac
     4381 gtcgagcgcc tgcgcaagac ctcgctcgag ctgctcgcgc ggcattgcca gctgatgctg
     4441 gactcgctcg ccgcgcccgc ggccaactga cgcctcgcgg cgcgcggcgc cgccggtccg
     4501 cctgtcggga gagccgcgcg cgacgccatg tccgcgccac tccgggattt gccctcatcc
     4561 gcgacagggg aaagtcttta agatccgtcg acatgccgct cgacgaaatc atggccatgg
     4621 tcggcgtcgc cgtccaggcg acgctcgcct tgctgctgct cgtggtctgg cgctcgctgc
     4681 gggcgcgctg gacgctgctg ctgtccgccg gcttcatcgc cgtgaccgcg cagtacttct
     4741 gcgtcgcgct cgggcattac acgcggggcg cgagctggca ggaaccgccc aagacgctga
     4801 acgcggccct ggcgctgctg gcccatggcc tgatcacctg ggcgctgatc gactacgtgg
     4861 gcctgccgcc ggcgtggagc cgccggctgc gccaggccac ggtgctggtg ttcggcctgg
     4921 cgttgatcgc gctggcggcg ggcatgctct cgcgcggcgc gacgatcggg gtcatcggca
     4981 cgttcatgtg cggctgggcc gcgttgttcg gctgggcgct gcgacgcgag ccgcgcgccg
     5041 ggcacggcat cgtgatgctg gcggtgctgg tctatccggt gtcgatgctg gccgcgctgg
     5101 agggctggtt cagcaccgag ctcgccggca tctcgttgat cgtgccgcat gcggtgctgg
     5161 gcatgaccat cctcaccacc ggcctcgtgc gcgcgcaggc gagcagcacg cgcgagctcg
     5221 ccgcgcgcca ggccgtgcag gccgagctgg agcgcaccaa ccagacgctg gagctgcgcg
     5281 tggcgctgcg caccgcgcag ctgcgcgaga cgatcgaagg cctggagagc ttcaaccgca
     5341 gcgtgtcgca cgacctgcgc gggccgctgg gcggcatcac cggcgtggcg cgcatggcgc
     5401 gcgaggcgct ggggcagggc gacctgaccg ccgccgacaa gctgctggcc gcgatcgcgc
     5461 gccagtccga gagctccacc gacctcgtca cgtcgctgct gcagctggcg cgctcgggcg
     5521 agggcccggt cgagcgccgc accgtgcaca ccgatgcgct ggtgcgagag ctcgccgacc
     5581 tgctggccgg cgccgcggcc gccacgcggc aggtggtggc catcgaaggc gagctgcccg
     5641 acctgcaggc cgaccccgcg ctggtgcgcc aggtgttcgc caacctgctg gccaacggca
     5701 tcaagttcag cgccagcgcg tcgcagccac gcgtcgacgt cggcgccacg cgcgggcccg
     5761 ggggtcacac cgtgttccac gtgcgcgaca acggcgtcgg cttcacggag gcgcaggggc
     5821 gcgagctgtt cgtcccgttc cgccgcctgc acgccgaccg cttcgagggc cacgggatcg
     5881 gcttgaccat cgtgcgccgc atcgtcgaac gccacggcgg ccgggtatgg gccgagagcc
     5941 gggcgggcga aggggcgacg ttcttcttct cgttcgagga gcagacctcc gacgcgaagc
     6001 ggcgcgaggt ggcctgacgg acgggttcgg gcggatgggt cgcgggccga ggggtccggc
     6061 ctctcctcga atcgcgccgc cacttggccg ccagcggatg tgcatgccgc gattcgaggc
     6121 caaggcctga ccccgatcga cgggagatcg gggtcaggcc ttcgcctcgg atccgcgacc
     6181 agcgcgtttg ccgatcgccg gccctgcgcg cggatccgag gagaggccag acccctccag
     6241 ccagaccccc tggcccgtcg ccagccgtcc cgccgcctca gcccagccgc gccttgtgcc
     6301 gcgcgatctg ccgctgcgcc tccaccggcg ccgtgccgcc ggtcacctgg cgcgcgttga
     6361 gcgagccgcg cagcgtcagc acgccgtaga cgtcctgtcc gatgctggcg tggaagccct
     6421 ggagcacgtc cagcggcagc tccgcgaggt ccttgcccga gtcgatgccg accttgaccg
     6481 cgtgcgccac cacctcatgg gcgtcgcgga agggcacgcc cttgcgcacg aggtagtcgg
     6541 c
//
LOCUS       DATCRO010000487        10112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_545677, whole genome shotgun sequence.
ACCESSION   DATCRO010000487 DATCRO010000000
VERSION     DATCRO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545677"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VIN75_13310"
     CDS             <1..498
                     /locus_tag="VIN75_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975194.1"
                     /translation="VHVEMSIVRPPIGVESHEAVVVDALVRAAARSSSGWVQLDLEAH
                     PSQRAFWRRLVRDARAALPASTRLSVTALAWWCRSDAWLDDLAADEVVPMFFRMGRDG
                     AAMRAALVDAPGRLHPRCRGEAVGLSLQEPVPAATVARFRRVYWFDNRHWRTPPAVPP
                     TALTP"
     gene            495..2765
                     /locus_tag="VIN75_13315"
     CDS             495..2765
                     /locus_tag="VIN75_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975195.1"
                     /translation="MILRLRPALLAALAATALCPAPSRASGDWSGPVDELVLRTDTDA
                     EHGDVLRGEIGIVLPTWDAAPLYLAWRAIVTGGKGAPPAAHAASAAASTPAGWTDVAA
                     QGDATSPFQQAGDGTNCRPDAWIFAQGTLQSLGRRPDATPARVQAWMAAQQRVFDLCN
                     RDPLDVLADADPQLAPPAPLPASEPLYWRQLRDYQLAAMAFYGAHYAQSEQAFARIGR
                     TAGHPMQPWGAYLALRSHLRALQLPDPAAPKPDPAQPPRVLPEQVDDLKALRREGAAI
                     LADPALAARHEDTAATLRRAAYLLAPNFHFAELADDLDDFKADPFKDDVLADWSFIGF
                     GVYNEFKPATGPLRARHAWFEWLAKVGRTDDSPVKGPPTCGSSCKSVLEAWHSSHAGS
                     GARRAWLLAALMSNAPLTAPMEQDARAVDAKAPEFLSVRYWLAARLLAAGRGDEARPL
                     TEDLVARLRQRKTPMPSALNLAIQQRFAEATSVADAAPWLLMRPLASITPDTGERAPV
                     PAMTTTMNPSPDGALWMDRGLAVRDMLALAQALKTPSPWRSRLAVAAWMRADLLGDAA
                     AATEASTLVEQFVPSLKPVTTRYRAQSGAPERRHLLLMAALRHNLSPDILGGNPDADA
                     TWPLQKPDEISAGMWCRIGADDAADYFPSAIGKTLPPLPAPVVSGDPARRDVERKRLF
                     DLHSATGTIGLHAIDWAAAHPADKDLPWLLHVAVASSRGGCVDADRSAISKKAWQVLH
                     KRFPRSEWTEQTPYFY"
     gene            2809..3246
                     /locus_tag="VIN75_13320"
     CDS             2809..3246
                     /locus_tag="VIN75_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY8975196.1"
                     /translation="MNPSTSLAFPAGAAAASHEEAHEEPPPGDLVTVTRVFNALEAEM
                     LRGCLEAEGIPATLGDVQTVQTDTLLTVAMGGIRVMVPTPFADAARQTVEAFERGALA
                     IDELPPGAESPFPVVEAEPAARLRLSRAAWIVLAVVVVAVVVF"
     gene            3346..3555
                     /locus_tag="VIN75_13325"
     CDS             3346..3555
                     /locus_tag="VIN75_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975197.1"
                     /translation="MNPLANRHDAADIGRPPPADAPMQVLPWAEDSTRSIGAEDAGIR
                     YQGDLFLPGWLRIELQPTAEQGPGG"
     gene            complement(3598..4173)
                     /locus_tag="VIN75_13330"
     CDS             complement(3598..4173)
                     /locus_tag="VIN75_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8975198.1"
                     /translation="MNTLSRLAAAALFAASAAQAAPVAYDIDPTHTYPSFEADHMGIS
                     TWRGKFDHTTGTVTMDRDAGTGTVDIVVDMKSVNFGLPALDKVARGKELFEVDKYPKA
                     HFKGTLEGFADGAPTKAVGTLEMHGKTNPVTLDIKKFKCIPHPMFKREDCGADIYATI
                     DREQFGMDAGKAYGFSMAVDLRIQVEAIARK"
     gene            4366..4914
                     /locus_tag="VIN75_13335"
     CDS             4366..4914
                     /locus_tag="VIN75_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8975199.1"
                     /translation="MLTVLLVIVVAVVALVAYASTRPDTFRIERSIRIGAPIIQVAEL
                     IDDFRAWSQWSPWEHIDPTMQRRYGGADTGVGAVYEWEGTGKVGAGRMEVTEMRAGPE
                     GGLISIKLDFLKPFKASNTAQFLLKPTDSGTDLTWAMFGPSPFMSKLMGVFMDMDKMV
                     GRDFEAGLESLKKTAEAAAKGS"
     gene            complement(5202..6701)
                     /locus_tag="VIN75_13340"
     CDS             complement(5202..6701)
                     /locus_tag="VIN75_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY8975200.1"
                     /translation="MNTIVANPAGSVPEAASRPWQPRGLAVIFFTEMWERFSFYGMRG
                     ILVLYLVKQLGYKDADANEVLGIYAGLVYVTPMIGGWIADRWLGMRLAAVIGGAVMML
                     GHFAMAVPSMLHIALGLLIVGNGFFKANTSSMVGELYDGVNDPRRDGGYSIFYMGINL
                     GAMLSPLVCGTLGEKVGWHYGFASAGVGMAIGVVTLVSFQGVIGRAGLREGQAPIGWS
                     NLPVIALWCLGSVGVVLAGLFIGPILGALPVAVKLVLALAAIAAAVLLPRMIGGKTVG
                     GDRLTGAEWKRIVAICVVTMFSVFFWMGFEQAGGSMNLFADQQTQRTLWGFEVPASWF
                     QTINSAAILVLGPLFALMWSWMNRSRYPLPDSAKQGLGLVILGLSFVLMDHAKVLADA
                     TGPVSPMWLFYAYLIQTLGELMLSPVGLALTSRAAPARVAALLMGVWMLSNAAGNYLA
                     GTLGKIMDGTGIAPYKFLYMAAIGAGVLLLLITPILHKMLRARDESTVI"
     gene            7046..8515
                     /locus_tag="VIN75_13345"
     CDS             7046..8515
                     /locus_tag="VIN75_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975201.1"
                     /translation="MPSPATLSCDLSAPATPLRHPWRHCVGSCHAPTALRADWQQQLT
                     RAHRELGFRHVRFHGLLSDDMGTLVNQDNQFLFSFFNIDRVFDFLLSLGMRPLVELSF
                     MPATLSSDGNSVFHYRANVTPPRRLADWQLLMTKLVGHWVERYGVDEVAQWPLEVWNE
                     PNLVAFWTGGQARYFEFFKATWTAVKAISPRLRVGGPATAANEWLADFDRFCAANACP
                     PDFISTHYYPTDAFGSILTDTVSQLADAPMSVMRDRARDARAVAGARPLYYTEWNITS
                     NPRDPMHDGPFCAALAAHFMLGVDDVVDGYSWWTFTDIFEENYFPSVPYHGGFGLMNL
                     YGVPKPVYRGFQLVDQLGTRRWPTTGSHPTLQMHVGAAADDAVTNVLLINVAMPRHPI
                     AGELVTVRLQGMQGRRPRSATITRIDDTHANAKAEWLRLGSPEYPHPRQVDALETASE
                     LAPERIAIAAEPGAIREVVFTLPMPANAMALVRIEWEAL"
     gene            8577..10073
                     /locus_tag="VIN75_13350"
     CDS             8577..10073
                     /locus_tag="VIN75_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8975202.1"
                     /translation="MTSPARPRDLGVDPNVAKHAVQHGFTRREAIARDTMLGDLQRES
                     FGYFVHEVNEANGLVLDKTAPDWPASVAAIGMALTCYPVGVQRGLMTRDKALQRTLVT
                     LRFLLAAEQGESPTAAGHRGFFYHFLDMATGRRAWRCELSTIDTALLMAGVLAAAAYF
                     RGPSDDEVELRRLAKALYERVEWDWMLGERGTICHGWKPETGRLPWHWEGYDEALVLY
                     LLALGSPTHPIPASSYDAWCSTYAWQEVEGVAYLHAGPLFIHQMSQLWIDFRGLQDGF
                     MRAHGSDYFANSRAAVQVQQRYAIRNPRRHAMYGEFCWGITASDGPGPQHWHGPGEPP
                     FFDYVARGVPEGPDDGTLAPWAVVASLPFAPDIVLPTVANYRHIQLHVANPYGFKASF
                     NPSYPADRRHAVGWVSPFHFGINEGPTVVMIENHRTGMPWKLMHGCAPLVAGLRRAGF
                     TGGWLDAIPRAAEAAPAAAMPAPEPLDPPTPPARTSVSQGPARRGTGG"
BASE COUNT         1464 a         3701 c         3506 g         1441 t
ORIGIN      
        1 gtccacgtgg agatgagcat cgtgcgaccg ccgatcggcg tcgaatcgca cgaggccgtc
       61 gtcgtcgacg cgctggtgcg cgcggccgcg cgatccagct ccggctgggt gcagctggac
      121 ctcgaggccc atccgtcgca gcgtgccttc tggcgccgcc tcgtgcgcga cgcgcgcgcg
      181 gcgctgccgg cgtcgacccg tctgagcgtc accgcgctcg cctggtggtg ccgttccgac
      241 gcctggctcg acgacctggc cgccgacgag gtggtgccga tgttcttccg catggggcgc
      301 gacggcgcgg cgatgcgcgc cgcgctcgtc gacgcgcccg gccgcctgca cccgcgctgc
      361 cgcggcgagg ccgtcggcct gtcgctgcag gagccggtgc ccgcggccac ggtcgcgcgc
      421 ttccggcgcg tctactggtt cgacaatcga cactggcgca cgccgccggc cgtcccgccc
      481 accgccctca cgccatgatc ctgcgacttc gacccgccct cctcgccgcc ctcgcggcca
      541 ccgcgctgtg cccggcgccc tcccgggcca gcggcgactg gagcggcccc gtcgacgagc
      601 tcgtgctgcg caccgacacc gatgccgagc acggggacgt gctgcgcggc gagatcggca
      661 tcgtgctgcc gacctgggac gccgcgccgc tgtacctggc ctggcgcgcg atcgtcaccg
      721 gcggcaaggg cgcgccgccc gcggcgcacg cggcatccgc ggcggcctcg acgccggccg
      781 gctggaccga cgtcgccgcg caaggcgacg ccacctcgcc gttccagcaa gccggcgacg
      841 gcaccaactg ccgccccgac gcatggatct tcgcgcaagg cacgctgcag tcgctgggca
      901 ggcgccccga cgcgacgccc gcgcgcgtgc aggcctggat ggccgcgcag caacgcgtgt
      961 tcgacctgtg caaccgcgac ccgctggacg tgctggccga cgccgacccg caactcgcgc
     1021 cgcccgcgcc gctgccagcg tccgagccgc tgtactggcg ccagctgcgc gactaccagc
     1081 tcgccgcgat ggccttctat ggcgcgcact acgcgcagag cgagcaggcg ttcgcgcgca
     1141 tcggtcggac ggcgggccac ccgatgcagc cgtggggcgc ctacctcgcg ctgcgctcgc
     1201 acttgcgcgc cctgcaactg cccgaccccg cggcgcccaa gcccgatccg gcccagccgc
     1261 cgcgggtgct gcccgagcag gtcgacgacc tgaaggccct gcggcgcgaa ggcgcggcca
     1321 tcctcgccga tcccgcgctg gcggcccgcc acgaggacac cgccgcgacg ctgcgccggg
     1381 cggcatacct gctggctccc aatttccatt tcgcggagct cgccgacgac ctcgacgact
     1441 tcaaggccga tccgttcaag gacgacgtgc tggccgactg gtcgttcatc ggtttcggcg
     1501 tctacaacga attcaagccg gccaccggtc cactgcgcgc caggcacgcc tggttcgaat
     1561 ggttggcgaa agtcggccgc accgacgact cgcccgtcaa agggccgccg acctgcggct
     1621 catcgtgcaa gagcgtgctg gaggcctggc actcgagcca cgccggatcc ggcgcgcggc
     1681 gcgcctggct cctcgccgcg ctgatgagca acgcgccgtt gaccgcgccc atggaacagg
     1741 acgcccgcgc ggtcgacgcg aaggcgcccg aattcctcag cgtccgctac tggctcgccg
     1801 ccaggctgct cgcggccgga cgcggcgacg aggcgcgacc gctgacggag gatctcgtcg
     1861 cccggctgcg ccagcgcaag acgccgatgc ccagcgcgct caacctcgcg atccagcagc
     1921 gcttcgccga ggcgacctcg gtggcggacg ccgcaccctg gctgctcatg cgcccgctgg
     1981 ccagcatcac gccggacacc ggcgagcgcg cccccgtccc cgccatgacc acgacgatga
     2041 acccgtcgcc ggacggcgca ctgtggatgg atcgcggcct ggcggtgcgg gacatgctgg
     2101 cgctggcgca ggcgctcaag acgccatcgc cctggcgcag ccgtctcgcg gtggcggcct
     2161 ggatgcgcgc cgacctgctc ggcgacgcgg ctgccgccac cgaggcgtcg acgctggtcg
     2221 agcagttcgt gccgtcgctc aagccggtga ctactcgcta ccgcgcgcag tcgggcgcgc
     2281 ccgagcgccg gcacctgctg ctgatggcgg cgctgcgcca caacctctcg ccggacatcc
     2341 tcggcggcaa ccccgacgcc gacgccacgt ggccgctgca gaagcccgac gagatctcgg
     2401 ccggcatgtg gtgccgcatc ggcgccgacg acgccgccga ctactttccc agcgcgatcg
     2461 gcaagaccct gccgccgctg ccggcaccgg tcgtctcggg cgatccggca cggcgcgacg
     2521 tcgagcgcaa gcgcctgttc gacctgcact cggccaccgg caccatcggg ctgcacgcga
     2581 tcgactgggc ggcggcgcac cccgcggaca aggacctgcc gtggctgctt cacgtggccg
     2641 tcgcgtcgtc gcgcggcggc tgtgtcgacg ccgaccgcag cgcgatctcg aagaaggcct
     2701 ggcaggtgct gcacaagcgc ttcccgcgca gcgagtggac ggagcagacg ccgtatttct
     2761 attgagcgga ctgacgcgcc tggggccgtt cgcgttcaga atgggcccat gaacccgtcg
     2821 acgtcgctcg cgttccccgc cggcgcggct gccgcgtcgc acgaagaggc gcacgaggag
     2881 ccgccgcccg gcgacctggt gaccgtcacc cgcgtgttca acgcgctgga agccgagatg
     2941 ctgcgcggct gcctggaggc cgaaggcatt cccgccacgc tgggcgacgt ccagacggtg
     3001 cagaccgaca cgttgctgac cgtcgcgatg ggcggcatcc gcgtgatggt cccgacaccc
     3061 ttcgccgacg cagcccggca gaccgtcgag gcgttcgagc gcggggcgct ggcgatcgac
     3121 gaactgccgc ccggggcgga gtcgccgttc cccgtggtcg aggccgagcc ggcagcccgg
     3181 ttgcgcctgt cgcgcgcggc ctggatcgtc ctggccgtcg tcgtggtcgc cgtcgtggtc
     3241 ttctgacccg atctggggat ggcgccgccg ccggtcgcgg gcggatgctc gcgggaccgg
     3301 ggcttgccgc ataccccgtg gccgccgcgc ggccgaggag tccgcatgaa tccgctcgcc
     3361 aaccgccacg acgccgccga catcggccgg ccgccgcccg ccgatgcacc gatgcaggtg
     3421 ctgccctggg ccgaggactc gacgcggtcg atcggtgccg aggacgccgg catccgctac
     3481 cagggcgacc tgttcctgcc gggctggctg cgcatcgagt tgcagccgac ggcggagcag
     3541 ggccccggcg gctgagcggc cgtcacgccg ccgtcacgtg cgtggcggcg acggccctca
     3601 cttgcgcgcg atcgcctcga cctggatgcg caggtcgacg gccatgctga agccgtaagc
     3661 cttgccggcg tccatgccga actgctcgcg gtcgatggtg gcgtagatgt cggcgccgca
     3721 gtcctcgcgc ttgaacatcg ggtgcgggat gcacttgaac ttcttgatgt ccagcgtgac
     3781 cgggttggtc ttgccgtgca tctccagcgt gccgaccgcc ttggtcggcg cgccgtcggc
     3841 aaagccctcc agcgtgccct tgaagtgcgc cttcgggtac ttgtcgacct cgaacagctc
     3901 cttgcccctg gccaccttgt ccagggccgg caggccgaag ttgacgctct tcatgtcgac
     3961 gacgatgtcc accgtgccgg tgccggcgtc gcggtccatc gtgaccgtgc cggtcgtgtg
     4021 gtcgaacttg ccgcgccacg tggagatgcc catgtggtcg gcctcgaagc tcgggtaggt
     4081 gtgcgtcgga tcgatgtcgt aggcgaccgg cgcagcctgg gcggcggagg cggcgaacag
     4141 ggcggcggcg gcgagacgag acagggtatt catcgggttc tccggtggtc gggtctgatg
     4201 ggaggtgact gtaatggcac ggtgccatgt ccccacgacg cgcgagccgt cccgttttcg
     4261 acatgggccc gcgacgacgc cggcgggaca cccctcttcc gccgggccgc atttttcgcc
     4321 gatgatcgcg acggccgcca cggccaggct tcgagggaac cgcccatgct caccgtcctg
     4381 ctcgtcatcg tcgtcgccgt cgtcgccctg gtcgcctacg ccagcacgcg gccggacacc
     4441 ttccgcatcg aacgctccat ccggatcggc gcgcccatca tccaggtcgc cgagctgatc
     4501 gacgacttcc gcgcgtggag ccagtggtcg ccatgggaac acatcgatcc gacgatgcag
     4561 cgcaggtacg gcggcgccga caccggcgtg ggggccgtct acgagtggga aggcaccggc
     4621 aaggtgggcg ccggccgcat ggaggtcacc gagatgcggg cgggcccgga gggcggcctc
     4681 atctcgatca agctcgattt cctcaagccc ttcaaggcga gcaacaccgc ccagttcctg
     4741 ctgaagccca ccgactcggg caccgacctc acctgggcga tgttcgggcc gagcccgttc
     4801 atgtccaagc tgatgggggt gttcatggac atggacaaga tggtgggcag ggatttcgag
     4861 gcagggttgg agtcgctgaa gaagaccgcg gaggcggcgg ccaagggttc ctgagtcctt
     4921 tgcgcgggcg ctccctggtc ccgcgcgact caccggatgg tgtcaggtct tgaaagttgc
     4981 gcgcccatgc gctccgtggc cgcgaagact tcctcgcgca actttcaaga cctgacacca
     5041 tcttcgacgg cgccggcgcg caacgattca agacctgaca ccacgacgcg gtggcgcgac
     5101 cgacaggcga cgcggcaggc cgccgtggat ccggcgactg cgcttcgcgc aggatgacgg
     5161 atcggctgcc ggtcggcgtt cccgtcgacg gcgcgcgagc gtcagatgac cgtcgactcg
     5221 tcgcgcgcgc gcagcatctt gtgcaggatc ggcgtgatca acagcagcag gacgccggca
     5281 ccgatggcag ccatgtagag gaacttgtag ggcgcgatgc cggtgccgtc catgatcttg
     5341 cccagcgtgc cggccaggta gttgccggcg gcattggaca gcatccagac gcccatcagc
     5401 agggcggcca cgcgggccgg tgccgcgcgc gacgtcagcg ccaggcccac cggcgagagc
     5461 atgagctcgc ccagggtctg gatcaggtag gcgtagaaca gccacatcgg gctgaccgga
     5521 ccggtggcgt cggccagcac cttggcgtga tccatcagca cgaagctcag acccaggatg
     5581 accaggccga ggccctgctt ggcggaatcg ggcagcggat agcgcgaccg gttcatccac
     5641 gaccacatca aggcgaacag cgggcccagc acgaggatgg cggcggagtt gatggtctgg
     5701 aaccagctgg ccggcacctc gaagccccac agcgtgcgct gggtctgctg gtcggcgaac
     5761 aggttcatcg acccgcccgc ctgctcgaag cccatccaga agaagaccga gaacatcgtg
     5821 accacgcaga tcgcgacgat gcgtttccac tcggcgccgg tcagcctgtc gccaccgacc
     5881 gtcttgccgc cgatcatgcg cggcaacagg acggccgccg cgatcgcggc gagcgccagc
     5941 acgagcttca cggcgaccgg aagcgcgccg aggatcgggc cgatgaacag ccctgcgagc
     6001 acgacgccga cggaacccag gcaccacagc gcgatgacgg gcaggttgct ccagccgatg
     6061 ggcgcctgcc cctcgcgcag gcccgcccgg ccgatcacgc cctggaacga caccaaggtg
     6121 acgacgccga tggccatgcc cacgccggcg gacgcgaagc cgtagtgcca gcccaccttc
     6181 tcgcccagcg tgccgcagac cagcggcgac agcatcgcgc cgaggttgat gcccatgtag
     6241 aagatgctgt agccgccgtc gcggcgcggg tcgttcacgc cgtcgtagag ctcgcccacc
     6301 atcgagctcg tgttggcctt gaagaagccg ttgcccacga tcagcaggcc gagcgcgatg
     6361 tgcagcatcg acggcacggc catggcgaag tggcccagca tcatcacggc cccgccgatg
     6421 acggccgcca ggcgcatgcc cagccagcgg tcggcgatcc agccgccgat catcggggtc
     6481 acgtacacca ggccggcgta gatgcccagc acctcgttgg cgtcggcgtc cttgtagccg
     6541 agctgcttga ccaggtacag caccaggatc ccgcgcatgc cgtagaagct gaagcgctcc
     6601 cacatctcgg tgaagaagat gacggcgagg ccgcgcggct gccagggtct ggacgccgct
     6661 tcaggcacgc tgccggcagg gtttgcaacg atggtgttca agtcgattcc cggggatcac
     6721 ggatggggaa cgccgcgccg acgccttttg ggcgccgcgc ggcggaagat cgcattgtcc
     6781 caaatcacca ccgaccggga tttggggctt gcccgggcac gggcatgcgg cctgccttcg
     6841 ccggcggcct gcctgcccgg ccacattcac gcgcgagatg tccgcttcga cgaacaagtt
     6901 cggccaatcg gcgttcaatt gcgccatgca gcgccgcgcc gcccttcgca agctcgccga
     6961 cggcctcgcc cttgctgtcg ggcattcgac ttgccgaccg gcgggcgcgg cgcctgtcga
     7021 gcggccgccg tcgtccatcg ccgccatgcc ctcgcccgcc accttgtcct gcgacctgtc
     7081 cgccccggcc acgccgctgc gccacccgtg gcgccattgc gtgggcagtt gccatgcgcc
     7141 caccgccttg cgcgcggact ggcagcagca gctcacccgc gcgcaccgcg agctgggctt
     7201 ccggcacgtg cgcttccacg gcctgctcag cgacgacatg ggcacgctcg tgaaccagga
     7261 caaccagttc ctgttctcgt tcttcaacat cgaccgcgtg ttcgacttcc tgctgtcgct
     7321 gggcatgcgc ccgctggtgg agctgtcgtt catgccggcc acgctgtcat cggacggcaa
     7381 cagcgtcttc cactaccggg ccaacgtcac gccgccgcgc cgcctggccg actggcagct
     7441 gctgatgacg aagctcgtgg gccactgggt ggagcgctac ggcgtcgacg aggtggcgca
     7501 gtggccgctg gaggtatgga acgagcccaa cctcgtggcg ttctggaccg gcggccaggc
     7561 gaggtacttc gagttcttca aggcgacctg gacggccgtc aaggcgatct cgccgcggct
     7621 gcgggtgggc gggccggcca cggcagccaa cgagtggctg gccgacttcg accggttctg
     7681 cgccgccaac gcctgtccgc ccgacttcat cagcacgcac tactacccga ccgacgcctt
     7741 cggctcgatc ctgaccgaca ccgtgtcgca gctggccgac gcgcccatga gcgtcatgcg
     7801 cgaccgcgcg cgtgacgcgc gggcggtcgc gggcgcgcgg ccgctgtact acaccgagtg
     7861 gaacatcacg tcgaatccgc gcgaccccat gcacgacggc ccgttctgcg ccgcgctcgc
     7921 cgcgcacttc atgctcggcg tggacgacgt ggtcgacggc tacagctggt ggaccttcac
     7981 cgacatcttc gaggagaact acttccccag cgtcccgtac cacggcggct tcgggctgat
     8041 gaacctgtac ggcgtgccca agcccgtgta ccgcggcttc cagctggtcg accagctcgg
     8101 cacgcggcgc tggccgacga cgggctcgca tccgacgctg cagatgcacg tcggcgccgc
     8161 cgccgacgac gcggtcacga acgtgctgct gatcaacgtc gcgatgccgc ggcacccgat
     8221 cgccggcgag ctcgtcaccg tgcggctgca gggcatgcag ggccgccggc cgcgctcggc
     8281 gacgatcacg cgcatcgacg acacccacgc caacgccaag gccgaatggc tgcgcctggg
     8341 cagccccgag tacccgcatc cgcgccaggt ggatgcgctg gagacggcct ccgagctggc
     8401 gcccgagcgc atcgccatcg cggccgagcc gggcgcgatc cgcgaagtcg tcttcacgct
     8461 gccgatgccc gccaatgcga tggcgctggt gcgcatcgaa tgggaggcct tatgagcgcc
     8521 cgcccggccg ctccgaaggg cgctcgcacc gcagcccgca gggcggaggt tgccttatga
     8581 cctcgcccgc ccgcccgcgc gacctcggcg tcgacccgaa cgtcgccaag cacgccgtgc
     8641 agcacggctt cacgcggcgc gaggcgatcg cgcgcgacac gatgctgggc gacctccagc
     8701 gcgagtcctt cggctacttc gtgcacgagg tcaacgaggc caacggcctg gtgctggaca
     8761 agacggcgcc ggactggccc gccagcgtcg cggcgatcgg catggcgctg acctgctatc
     8821 cggtgggcgt gcagcgcggc ctgatgacgc gcgacaaggc gttgcagcgc acgctcgtga
     8881 cgctgcggtt cctgctggcc gccgaacagg gcgagtcgcc gaccgcggcc ggccatcgcg
     8941 gcttcttcta ccacttcctg gacatggcca cgggacgccg cgcgtggcgg tgcgagctgt
     9001 cgacgatcga caccgcgctg ctgatggcgg gcgtgctggc cgcggccgcg tacttccgcg
     9061 ggccgtcgga cgacgaggtc gagctgcggc gcctggccaa ggcgctgtac gagcgcgtcg
     9121 aatgggactg gatgctcggc gagcgcggca cgatctgcca cggctggaag cccgagacgg
     9181 ggcggctgcc ctggcactgg gagggctacg acgaggcgct ggtgctgtac ctgctggcgc
     9241 tgggctcgcc gacgcacccg atcccggcgt cgtcgtacga cgcgtggtgc agcacgtacg
     9301 cgtggcagga ggtcgagggc gtcgcctacc tgcacgcggg gccgctgttc atccaccaga
     9361 tgtcgcagct gtggatcgac ttccgcgggc tgcaggacgg cttcatgcgc gcgcatggca
     9421 gcgactactt cgccaacagc cgcgccgccg tgcaggtgca gcagcgctac gcgatccgca
     9481 atccgcgccg ccacgcgatg tacggcgagt tctgctgggg catcaccgcc agtgacgggc
     9541 cgggcccgca gcactggcac gggccgggcg agccgccgtt cttcgactac gtcgcgcgcg
     9601 gcgtgcccga gggccccgac gacggcacgc tggcgccgtg ggccgtcgtc gcgtcgctgc
     9661 cgttcgcgcc ggacatcgtg ctgcccacgg tcgccaacta ccgccacatc cagctgcacg
     9721 tggccaaccc gtacggcttc aaggcctcgt tcaacccgag ctatccggcc gaccgccggc
     9781 acgcggtcgg ctgggtgtcg ccgttccatt tcggcatcaa cgaagggccg accgtcgtga
     9841 tgatcgagaa ccaccgcacc ggcatgccgt ggaagctgat gcatggttgc gcgccgctgg
     9901 tcgccggcct gcgccgcgcg ggcttcacgg gcggatggct cgacgcgatc ccgcgcgcgg
     9961 ccgaggccgc ccccgcggcg gccatgcccg cgccggagcc actcgatccg ccgacgccgc
    10021 cggcgcggac gtcggtgtcg caggggccgg cgcgacgagg caccggcggt tgaggaaagg
    10081 ccgtggcatc gcgcgaccgg aggggcctgg cc
//
LOCUS       DATCRO010000488         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_488842, whole genome shotgun sequence.
ACCESSION   DATCRO010000488 DATCRO010000000
VERSION     DATCRO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488842"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VIN75_13355"
     CDS             complement(<1..1009)
                     /locus_tag="VIN75_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEY8975203.1"
                     /translation="MRLPAHVLNGIAVSLGISLIQITFALAFGKLAALAAATGAICSS
                     LADLPIAPARTWRRVGTGAVMACLSVLLVNLLRESGVAMGITVMFLAFCSAMALAWGL
                     RAGPLSFIPILALIFTLAAPPPANMRALWMHCGWTAVGALAYFLWAVLGSRVLQPRYR
                     TLALAAALSALATLLRSRAALLSRTESNGPPPLQEWIRSQVALDERLQAARDLLFPAV
                     GEPHAGRAIAVLLQAVEMRDTLLAGELDIELLGGDAPAHQLRERLRVHGGRVADAIEA
                     MADALRDHGMAVPASAATPFDETDIETGEPVTPHPSRHVPGSASLARSAASAAATAPS
                     SA"
     gene            1121..1822
                     /locus_tag="VIN75_13360"
     CDS             1121..1822
                     /locus_tag="VIN75_13360"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY8975204.1"
                     /translation="MTPASRLALLCALAFAAARAPAAPPLPKDKRQALADFVVAYRLA
                     EAWPRMAPKIAHDSLPRLEDATHADLDADKFPDRAASEAAHARVPALLAEGRRELEAA
                     LQRFDADELAAYTAYEIYAKYFETEEIRQISAFFDSPTGRKLSALGPTLIAQSRRPGA
                     GNVMPNHFDGAELGEIAAFWNSPVGRKMNETAEQIREDMHAHFIERSEPAVQAVARDL
                     ASKAELQAAAASAGR"
     gene            1985..3115
                     /locus_tag="VIN75_13365"
     CDS             1985..3115
                     /locus_tag="VIN75_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HEY8975205.1"
                     /translation="MSSLPPSSTPPEGDDSAPVSLPAADAAQESAPVAAAAAEPSSEA
                     DAAAAGASGDTPAAEESQPAGQAAKPALEDAGPQLKKLYPALFSNPPKPLKLRIQADI
                     QARSPGQFTKPQLSAFLRRYTGSHAYLNALVKATHRFDLDGNAGDELSDEHRNAAVEE
                     LARRRANTQARMALEQEQRANRATLLKEFEANTLPAAEFAAAKGIPEAELEPLIARAR
                     KEASEPPPPAFNRDRRGPQHKRGPRPEGQRGARPSRDAQSAPASEGEAAQAEGERAAR
                     APRPPRADGPRGPRPEGQRGPRGDRPQGPRADGPRGPRPDGPRGPRPEGDRPRNAGPR
                     RDDDRRPRQDDRPQRANVSSSPAAPTAMALAFGALANLKTKK"
     gene            complement(3304..>3668)
                     /locus_tag="VIN75_13370"
     CDS             complement(3304..>3668)
                     /locus_tag="VIN75_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY8975206.1"
                     /translation="CGDGVQEMYRAICALRGEKARRIGLKPLLGHACTARDPHCSRAL
                     AMFCGLLGDFAGHVALTLGADGGVVVAGEIVDALGHWFTRSPFRRRFEARGRYRETLR
                     AIPTVVAGRGAQPRLMVG"
BASE COUNT          551 a         1445 c         1282 g          390 t
ORIGIN      
        1 ccgccgacga cggcgcggtg gccgcggcgg acgcggccga acgcgccagc gatgccgagc
       61 ccggcacgtg gcgcgacgga tgcggcgtga cgggctcgcc ggtctcgatg tccgtctcgt
      121 cgaacggcgt cgcggccgac gcgggcaccg ccatgccgtg atcgcgcagc gcatcggcca
      181 tcgcctcgat ggcgtcggcc acgcggccgc catgcacgcg caggcgttcg cgcagctggt
      241 gggcgggcgc gtcgccgccc agaagctcga tgtcgagctc gcccgcgagc agcgtgtcgc
      301 gcatctcgac ggcctgcagc agcacggcga tcgcccggcc ggcgtgcggt tcgccgaccg
      361 ccgggaacag caggtcgcgc gcagcctgca ggcgctcgtc gagcgcgacc tggctgcgga
      421 tccactcctg cagcggcggc ggcccgttgg actcggtgcg cgacagcagc gccgcacgcg
      481 agcgcagcag cgtcgccagc gccgacaacg cggcggccag cgccagcgtg cggtagcgcg
      541 gctgcagcac gcgcgagccc agcacggccc acaggaagta cgcgagcgcg ccgacggccg
      601 tccagccgca gtgcatccac agcgcgcgca tgttggccgg cggcggcgcg gccagcgtga
      661 agatcagcgc caggatcggg atgaacgaca gcggccccgc gcgcaggccc cacgccagcg
      721 ccatcgccga acaaaacgcc aggaacatca cggtgatgcc catcgccacg cccgactcgc
      781 gcagcaggtt gaccagcagc acgctcaggc aggccatcac cgcgccggtg cccacgcggc
      841 gccaggtgcg cgccggcgcg atcggcaggt cggccaggct cgagcagatg gcgccggtcg
      901 ccgcggccag cgcggccagc ttgccgaacg ccagcgcgaa cgtgatctgt atcagggaga
      961 tgcccagcga cacggcgatt ccgttgagca cgtgcgcggg cagacgcatg agcaagcgtg
     1021 gggtctccat gcgggccatt gtggcgcacg cgccgtgtgc tcgcgagcgc gcccgcgcct
     1081 cacgcgcgag aatgcgcgtc accgttcgag atcgccgccg atgacgcccg cctcccgcct
     1141 cgccctgctc tgcgccctcg cgttcgccgc cgcgcgcgcg cccgccgcgc cgcccctgcc
     1201 caaggacaag cgccaggccc tggcggactt cgtcgtcgcc taccggctgg ccgaggcgtg
     1261 gccgcgcatg gcgcccaaga tcgcgcacga ttcgctgcca cgcctggagg acgccacgca
     1321 cgccgacctg gacgccgaca agttccccga ccgtgccgcg tccgaggcgg cgcacgcccg
     1381 cgtccccgcg ctgctggccg agggccggcg cgagctcgag gccgcgttgc agcgcttcga
     1441 cgccgacgag ctggccgcgt acaccgccta cgagatctac gcgaagtact tcgagaccga
     1501 ggagatccgc cagatcagcg cgttcttcga ctcgccgacg gggcgcaagc tgtcggcgct
     1561 cggcccgacg ctcatcgcgc agtcgcgcag gccgggcgcc ggcaacgtca tgcccaacca
     1621 cttcgacggc gccgaactcg gcgagatcgc ggcgttctgg aattcgcccg tgggccgcaa
     1681 gatgaacgag accgcggaac agattcgcga ggacatgcac gcgcacttca tcgagcgctc
     1741 ggagccggcg gtgcaggcgg tggcgcgcga cctggcgagc aaggcggaat tgcaggcggc
     1801 ggccgcgagc gcggggcgct gacgccggcc ggcgatcccg tcgcgccagc ggcagaagag
     1861 ctctcgcgac gccagctcga cagcccctcg caccgcccgt ttcaaacccg cgaaccgccg
     1921 ggctgtcgcg gcgccccgaa cattgcgaca atacgcccct tcaccgcttc acttccgttg
     1981 ccgcatgtcc tcgcttcccc cgtcctcgac gccgcccgaa ggcgacgatt ccgcgcccgt
     2041 ctcgctgccc gcagccgacg cggcgcaaga gtcggcgccc gtcgctgccg ccgctgccga
     2101 gccgtcgtcc gaggcggacg ccgccgcggc cggtgcatcg ggcgacaccc ccgccgccga
     2161 ggaatcgcag ccggccggcc aggccgcgaa gcccgcgctg gaggatgccg gcccgcagct
     2221 caagaagctg tacccggcgc tgttctcgaa cccgcccaag ccgctgaagc tgcgcatcca
     2281 ggccgacatc caggcgcgct cgccgggcca gttcaccaag ccgcagctgt cggccttcct
     2341 gcgccgctac accggcagcc acgcgtatct gaacgcgctg gtcaaggcca cgcaccgctt
     2401 cgacctggac ggcaacgccg gtgacgagct gagcgacgag caccgcaacg cggccgtcga
     2461 ggagctggcg cgccgccgcg cgaacaccca ggcgcgcatg gcgctcgaac aggagcagcg
     2521 cgccaaccgc gccacgctgc tcaaggagtt cgaggccaac acgctgcccg ccgcggagtt
     2581 cgccgccgcc aagggcatcc ccgaggccga gctcgagccg ctgatcgccc gcgcccgcaa
     2641 ggaagcgtcg gagccgccgc cgcccgcgtt caaccgcgat cgccgcggcc cgcagcacaa
     2701 gcgcggcccg cgaccggaag gacaacgtgg cgctcgtcca tcgcgcgatg cgcagtccgc
     2761 gcccgcgagc gaaggcgagg ccgcgcaggc cgaaggcgag cgcgcggctc gggcgccgcg
     2821 tccgccgcgc gccgacggcc cgcggggtcc gcgtccggaa ggccagcgcg gtccgcgcgg
     2881 cgatcggccg caaggcccgc gtgcggacgg cccccgtggt ccgcgtccgg atggccctcg
     2941 cggcccgcgc ccggaaggcg accgtccgcg caacgccggt ccgcgtcgcg acgacgaccg
     3001 ccgcccgcgc caggacgatc gtccgcagcg cgcgaacgtc tcgtcgagcc cggcagcgcc
     3061 caccgcgatg gcactggcgt tcggcgcgct ggccaatctc aagacgaaga agtgaccggc
     3121 acgacagcca tggccgcgcg ctgaccgatc ggtgcgcgga tccctcgccc gcacggcgag
     3181 acgctgccga aggggtctgg ctccgccctg actcgcggcc gccacgccga tgacatcggc
     3241 tgcggtgcct gacgccgcct tcgatgcggc ggcctgagaa cgactcgata cggagccagc
     3301 ggttcagccg accatcaacc gcggctgcgc ccctcgcccc gccaccaccg tcggaatcgc
     3361 ccgcagcgtc tcgcgatagc gcccgcgcgc ctcgaaccgg cggcggaacg gcgagcgggt
     3421 gaaccagtgg ccgagcgcat cgacgatctc gcccgcgacc accacgccgc cgtccgcgcc
     3481 cagcgtcagc gcgacgtgtc cggcgaagtc gccgagcagg ccgcagaaca tcgccagcgc
     3541 ccgcgagcaa tgcgggtcgc gcgcagtgca cgcgtgcccg agcagcggct tcagtccgat
     3601 gcggcgggcc ttctcgccgc gcaacgcgca gatcgcgcgg tacatctcct gcacgccgtc
     3661 gccgcaca
//
LOCUS       DATCRO010000489         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_781422, whole genome shotgun sequence.
ACCESSION   DATCRO010000489 DATCRO010000000
VERSION     DATCRO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781422"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VIN75_13375"
     CDS             <1..576
                     /locus_tag="VIN75_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8975207.1"
                     /translation="AQVEPHFLFNTLANVQALVETGSPRAPAVLRSLIAYLKATMPRL
                     DDGDARLGREMTLVRSYLELMHLRMPDRLQFTVEGDPALQGFPCPPMLLLTLVENAVR
                     HGIDPSETGGAIAVSTSRSGDIVRLAVRDDGVGMGESAVPGTGLANLRERLVAFYGPA
                     ARLELSEVAPHGLLAEILIDVPASHATQATS"
     gene            573..1487
                     /locus_tag="VIN75_13380"
     CDS             573..1487
                     /locus_tag="VIN75_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8975208.1"
                     /translation="MSDSEFPVDDTAARPRGPGAPGAVRALIVDDEPLLRTHLAHHLA
                     QLWPELEIAGEARNGREAVDLVVQLRPDVVFLDVHMPGMNGVEAARLMGPDVQIVFVT
                     AYEQYAVAAFEQGAIDYVVKPFDAERLGESVARLRRRVAERRRMNVADGGPGQGAGLA
                     ADAPEASAALGRLPPGDLDAVLSALAGELRRRAPAGDRLQWIRASVGQMIKLIPVDEV
                     LYFRSDEKYTLVVWVGGEALIRKSIRELLDGLEPETFIQIHRSTIVNLRFVSQVVKGP
                     NETAEVHLKGRSETLSVSRNHVHWFRQY"
     gene            complement(1512..3053)
                     /locus_tag="VIN75_13385"
     CDS             complement(1512..3053)
                     /locus_tag="VIN75_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY8975209.1"
                     /translation="MSETDLEVQPGEAAPVRGGRAPVRRWILQGARSVVFLRPDWTGL
                     RATPLFLAGVCAANLAVVIATGRLFVAGPATFHWYLLGYGWFQAVLVAWGCWLASNGA
                     APRGDSPDTSVDAPAMLTMLWVQCLAFAVLAGIVYVPAWRGVGGLHWPTGALTYMPLL
                     VHVAWMASAQIRVALGGTRAARPWPRAGLVLALLLALALDLAIPLRYWQADKAPQSTA
                     RAAEPAAVASAAASTPDGGPDATEDADAETDFPLLKLSQEMFEHQQRLLATQLDAIAP
                     QRPGVVDMYAITFAPYADVDVFMRESAVVAGVMEQRFDTRGRTLQLVNNLKTTDTLPW
                     ATPLNLQRAIARMARRMDRDDDILFIHLTSHGGADARLAAEFFPLTVDELTPQALRRW
                     LDEAHVRNRVISISACYSGSWIAPLADDDTLVMTAADADHTSYGCGSKSTLTFFGQAM
                     YDEQLRHTWSFEEAHAASRKLIAQREKEAGKTDGYSNPQIRMGSRIRERLKALQARLA
                     GSAAR"
     gene            3251..>3742
                     /locus_tag="VIN75_13390"
     CDS             3251..>3742
                     /locus_tag="VIN75_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975210.1"
                     /translation="MSHTPSRKNFSVAQRLAFGFGTLVALSLLGSAVSAWQAQVIAAK
                     VERVVEVNNAMSDAVGHLRNSMDEMAIQVRGVALMTEMKSITAEVEVFKAAKARYLAD
                     EKTLQALAAGAGGNDDERRTVKDIAALAAQTIPMLQETADQAADGNNVEAALSLTNRI
                     LPLE"
BASE COUNT          539 a         1342 c         1352 g          509 t
ORIGIN      
        1 gcgcaggtcg agccgcattt cctgttcaac acgctcgcca acgtgcaggc gctggtcgag
       61 accggttcgc cgcgcgcgcc cgccgtgctg cgcagcctga tcgcctacct caaggccacg
      121 atgccgcgcc tggacgacgg cgacgcccgc ctgggccgcg agatgacgct ggtgcgcagc
      181 tacctcgagc tgatgcacct gcgcatgccc gaccgcctgc agttcaccgt ggagggcgac
      241 ccggcgctgc agggcttccc gtgcccgccg atgctgctgc tgacgctggt ggagaacgcc
      301 gtgcgccacg gcatcgaccc atccgagacc ggcggcgcga tcgcggtgag cacttcgcgc
      361 tcgggcgaca tcgtgcgcct ggcggtgcgc gacgacggcg tgggcatggg cgagtcggcg
      421 gtgcccggca ccggcctcgc caacctgcgc gagcgcctcg tcgcgttcta tggcccggcg
      481 gcccggctgg agctgtccga ggtcgcgccg cacgggctgc tggccgagat cctgatcgac
      541 gtgcccgcgt cgcacgccac ccaagccact tcatgagcga ttccgaattt cccgtcgacg
      601 acaccgccgc acgcccgcgc ggccccggcg cgcccggcgc cgtgcgcgcg ctgatcgtcg
      661 acgacgagcc gctgctgcgc acgcatctgg cccaccacct ggcgcagctg tggccggagc
      721 tcgagatcgc cggcgaggcg cgcaacggcc gcgaggcggt cgacctcgtg gtgcagctgc
      781 ggcccgacgt cgtcttcctg gacgtgcaca tgccgggcat gaacggcgtc gaggccgcgc
      841 gcctgatggg gccggacgtg cagatcgtgt tcgtcaccgc ctacgagcag tacgccgtcg
      901 cggcgttcga gcagggggcg atcgactacg tcgtcaagcc cttcgacgcc gagcgcctgg
      961 gcgagagcgt ggcccgcctg cgccgccgcg tcgccgagcg ccgccgcatg aacgtggccg
     1021 acggcgggcc gggccagggg gcgggcctgg cggccgatgc gcccgaggcc tcggccgcgc
     1081 tcggccggct gccgcccggc gacctcgacg ccgtcctcag cgcgctcgcc ggcgagctgc
     1141 gccgccgcgc gcccgcgggc gaccggctgc agtggatccg cgcgtcggtc ggacagatga
     1201 tcaagctgat cccggtcgac gaggtgctgt acttccgttc cgacgagaag tacacgctgg
     1261 tcgtgtgggt gggcggcgag gcgctgatcc gcaagtcgat ccgcgagctg ctggatggcc
     1321 tcgagcccga gaccttcatc cagatccacc ggtcgaccat cgtcaacctg cgcttcgtgt
     1381 cgcaggtggt caaggggccc aacgagacgg ccgaggtgca cctgaagggt cgcagcgaga
     1441 cactgagcgt cagccgcaac cacgtgcact ggttccgcca atattgagcg gcagggcgcg
     1501 atcgcgggac gtcagcgcgc cgcggagccc gccagccgcg cctgcagggc cttgaggcgc
     1561 tcgcggatgc gcgagcccat gcggatctgc ggattcgagt agccatccgt cttgcccgcc
     1621 tccttctcgc gctgcgcgat gagcttgcgc gacgcggcgt gggcctcctc gaacgaccat
     1681 gtgtggcgca gctgctcgtc gtacatggcc tgtccgaaga aggtcagcgt cgacttcgag
     1741 ccgcagccgt aggacgtgtg gtcggcgtcg gccgcggtca tcacgagcgt gtcgtcgtcg
     1801 gccagcggcg cgatccagct gcccgagtag caggccgaga tcgagatgac gcggttgcgg
     1861 acgtgcgcct cgtccagcca gcggcgcagc gcctgcggcg tcagctcgtc caccgtgagc
     1921 gggaagaact ccgcggccag ccgcgcatcg gcgccgccgt gcgacgtcag gtggatgaac
     1981 aggatgtcgt cgtcgcggtc catgcgccgg gccatgcgcg cgatggcgcg ctgcaggttc
     2041 agcggggtcg cccagggcag ggtgtccgtc gtcttgaggt tgttgaccag ctgcagcgtg
     2101 cggccgcgcg tgtcgaagcg ctgttccatg acgcccgcga cgaccgcgct ctcgcgcatg
     2161 aagacgtcga cgtccgcata gggcgcgaag gtgatggcgt acatgtcgac gacgcccggg
     2221 cgctgcggcg cgatggcgtc gagctgcgtg gccagcagcc gctgctggtg ctcgaacatc
     2281 tcctgcgaga gcttgagcag cgggaagtcc gtctcggcgt cggcgtcctc ggtggcgtcc
     2341 gggccgccgt ccggcgtgct ggcggcagcg ctcgccaccg cggccggctc cgccgcccgg
     2401 gccgtcgact gcggcgcctt gtcggcctgc cagtagcgca acgggatcgc gagatccagg
     2461 gccagggcca gcagcagggc cagcaccagg ccggcgcggg gccaggggcg cgcggcgcgc
     2521 gtgccaccga gcgccacgcg gatctgtgcc gatgccatcc atgcgacatg gacgagcagg
     2581 ggcatgtacg tcagcgcacc ggtcggccag tgcaggccgc cgacgccgcg ccatgcgggc
     2641 acgtagacga tgccggccag gacggcgaag gccaggcact gcacccacag catcgtcagc
     2701 atggcaggag cgtcgacgga cgtgtccggc gagtcgccgc gcggcgccgc cccgttcgag
     2761 gcgagccagc agccccacgc gaccagcacg gcctggaacc atccgtagcc cagcaggtac
     2821 cagtggaagg tcgccgggcc tgccacgaac aggcggccgg tcgcgatcac cacggcgagg
     2881 ttcgcggcgc agacacccgc gaggaacagc ggcgtggcgc gcagtcccgt ccagtccggc
     2941 cgcaggaaca cgacgctgcg cgcgccctgc aggatccagc gcctcaccgg cgcacggccg
     3001 cctcgtaccg gggccgcctc gccgggctgc acctccagat cggtctccga catcgcgctc
     3061 cccctgtttc ccggcacatt gtccgcgaag cggcgtgcgc tgcccgaacg gctcaagcgg
     3121 cggcctgatc ggccgatcgc gtgcgcggcc cggcgcgggt gagaaatatg cgcggcgacg
     3181 gcgatcccgg atgctcaatt tccggcgggg accgccgata gctcggggaa tcccctgtca
     3241 ggcactctcc atgtcgcaca ctccctcccg caagaacttc tccgtcgcgc agcgactcgc
     3301 cttcggcttc ggcacgctgg tggcgctgtc gctgctgggg tcggccgtca gcgcctggca
     3361 ggcgcaggtg atcgccgcca aggtcgagcg cgtcgtcgag gtcaacaacg cgatgtccga
     3421 cgcggtgggc cacctgcgca actcgatgga cgagatggcc atccaggtgc gcggcgtcgc
     3481 gctgatgacc gagatgaagt cgatcacggc ggaggtcgag gtgttcaagg ccgccaaggc
     3541 gcgctacctc gccgacgaga agacgctgca ggcgctggcc gccggcgccg gcggcaatga
     3601 cgacgaacgc cgcacggtca aggacatcgc cgcgctcgcc gcgcagacga tcccgatgct
     3661 gcaggagacc gcggaccagg ccgccgacgg caacaacgtc gaggccgcgc tgtcgctgac
     3721 caaccgcatc ctgccgctcg ag
//
LOCUS       DATCRO010000490         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_803911, whole genome shotgun sequence.
ACCESSION   DATCRO010000490 DATCRO010000000
VERSION     DATCRO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803911"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VIN75_13395"
     CDS             complement(<1..912)
                     /locus_tag="VIN75_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY8975211.1"
                     /translation="MNKPTHLLALDQGTSSSRSIVFDAQGGIVAMAQAEFRQIYPRPG
                     WVEHDPREILQSQLATAREALAKAGLTAADIASIGITNQRETTLVWNRITGEPVHNAI
                     VWQDRRAEPTCASLREQGLEPVFRAKTGLIVDAYFSGTKLKWILDNVDGARAAAARGE
                     LAFGTIDTWLIWHLTGGKVHATDVSNASRTLLLDVHANAWSDELLKLLGVPRNMLPDV
                     RPSAHLFGHTKEEVLGVSLPIGGVAGDQQSALFGQACFRPGLAKNTYGTGCFMLMHTG
                     SKFQLSTNGLVTTSASQPTATPEFALEG"
     gene            complement(909..1685)
                     /locus_tag="VIN75_13400"
     CDS             complement(909..1685)
                     /locus_tag="VIN75_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY8975212.1"
                     /translation="MNPSPRQLELIEEVRRMQTVSVEALAERFGVTLQTVRRDIRQLN
                     DAGLLTRFHGGVRMPGSTTENIEYRKRQQLNAQAKQRIAHEVAARVPHGSSLLINIGT
                     TTEAIARALLRHKGLRVITNNLNVAAILSDNADCEVIVAGGAVRAMDRGIIGEMTVDF
                     IGQFKVDIGLIGISGIEADGSLRDFDYREVKVARAILEHSREVWVAADHSKFNRPAMV
                     EMARFEQIEMLFTDGAPPEPFPALLAQANVTLVNCNDEQP"
     gene            1817..3406
                     /gene="glpD"
                     /locus_tag="VIN75_13405"
     CDS             1817..3406
                     /gene="glpD"
                     /locus_tag="VIN75_13405"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY8975213.1"
                     /translation="MQAASPTSGPRSGALPTQCDVLVVGGGINGAGIARDLAGRGLQV
                     VLCEKDDLAQATSSSSTKLIHGGLRYLEYNEFALVRKALAEREVLLRSAPHIMWPLRF
                     VMPHDPGMRPVWMIRIGLFLYDHLARREVLPGSRTVDLRRHAAGAPLQPRFTKGFVYS
                     DGWVDDARLVVLNAIDAMERGATILTRWHCRDAQRSADGWQATLDSADGSFHTLKARA
                     LVNAAGPWAAQFLSEHAHLSRNKSLRLVKGSHIVVPKLFEHGHAYIFQNPDKRIIFAI
                     PYEHDFTLIGTTDVEHHGPVGAARIDESEIRYLCEQASRYFAKAVVPADVVWTYSGVR
                     PLLDDESGDPSAVTRDYSLDLDTAQAPLLTVWGGKITTFRRLAEDAADLLMAPLGVDS
                     RRGRWTNGAFLPGGDLNKWIGPARRPDTDFVRFEETVRRRYPQFDAAVLHRLARAYGA
                     RVDRVLTDTPGAEVAPGLFECELAYLHDHEWARSADDVLWRRSKLGLHLSAEERAAVA
                     AWCTEHWAGATATSTPETAWN"
     gene            3397..4464
                     /locus_tag="VIN75_13410"
     CDS             3397..4464
                     /locus_tag="VIN75_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975214.1"
                     /translation="MELMLERIEQRAGAQAHLYPLDLKLVPNAITVLLGATQAGKTTL
                     MRVMAGLDKPHAGRVLVDGADVTGKPVRDRNVAMVYQQFINYTSMTVADNIASPLRLR
                     KETDIDRRVLELARKLHIEPFLKRMPGELSGGQQQRVALARALAKDAPLMLFDEPLVN
                     LDYKLREELRDELSALFAAGRSTVVYATTEPTEALLLGGYTAVMDAGELLQYGPTAEV
                     FHRPKSIRVARAFSDPPMNLITGQASPQGVALPGGALLACTLPPGAPGALTVGVRAGA
                     LGVRERPGDLALAGVVELAEISGSDTFVHVKTAVGELVAQLTGVHVFALGSNVTLYLH
                     PGQAYVFGQDGNLLVAPTENH"
     gene            4464..>4993
                     /locus_tag="VIN75_13415"
     CDS             4464..>4993
                     /locus_tag="VIN75_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975215.1"
                     /translation="MSRIDLDLAHAYKPDPKTDDDYALLPLKMTFDDGGAYALLGPSG
                     CGKTTLLNIVSGLVRPSQGTVKYDGKDVTGLTPQQRNIAQVFQFPVIYDTMTVAENLA
                     FPLRNRGVARDRIKDRVGRIAEMLEMSHQLDSRAAGLAADAKQKISLGRGLVRQDVSA
                     ILFDEPLTVIDPHLKW"
BASE COUNT          753 a         1763 c         1733 g          744 t
ORIGIN      
        1 gccctccagc gcgaactccg gcgtggccgt cggctgcgag gcgctggtgg tgacgagccc
       61 gttggtcgag agctggaact tggacccggt gtgcatcagc atgaagcagc cggtgccgta
      121 ggtgttcttc gccaggccgg gccggaagca ggcctggccg aacagcgcgc tctgctggtc
      181 gccggccacg ccgccgatgg gcagggacac gcccagcacc tcttccttcg tgtggccgaa
      241 gaggtgggcc gagggccgca cgtcggggag catgttgcgc ggcacgccca gcagcttcag
      301 cagctcgtcg ctccaggcgt tggcgtgcac gtccagcagc agcgtgcgcg aggcgttgct
      361 cacgtcggtc gcgtgcacct tgccgccggt caggtgccat atcagccagg tgtcgatggt
      421 gccgaacgcg agctcgcccc gcgcggcggc ggcgcgcgcg ccgtcgacgt tgtcgaggat
      481 ccacttgagc ttggtgccgg agaagtaggc gtcgacgatc aggcccgtct tcgcgcggaa
      541 caccggctcc aggccctgtt cgcgcagcga cgcgcaggtg ggctcggcgc ggcggtcctg
      601 ccacacgatc gcgttgtgca ccggctcgcc cgtgatgcgg ttccacacga gcgtggtctc
      661 gcgctggttg gtgatgccga tggaggcgat gtcggccgcc gtcagtccgg ccttggcaag
      721 cgcctcgcgg gccgtggcca gctgcgactg caggatctcc ctcgggtcat gctccaccca
      781 gcccggacgc gggtagatct ggcggaactc ggcctgcgcc atcgcgacga tgccgccctg
      841 ggcgtcgaac acgatcgagc gcgagctgga ggtgccctgg tcgagcgcga ggaggtgggt
      901 gggcttgttc atggttgttc gtcgttgcag ttgacgagcg tcacgttcgc ctgcgcgagc
      961 agcgcgggga agggctcggg cggcgcgccg tcggtgaaca gcatctcgat ctgctcgaag
     1021 cgcgccatct ccaccatcgc ggggcggttg aacttgctgt ggtcggcggc cacccagacc
     1081 tcgcgcgaat gctcgaggat ggcgcgcgcc accttgacct cgcggtagtc gaagtcgcgc
     1141 agcgagccgt cggcctcgat gcccgagatg ccgatcagcc cgatgtcgac cttgaactgg
     1201 ccgatgaaat cgaccgtcat ctcgccgatg atgccgcggt ccatcgcgcg caccgcgccg
     1261 ccggcgacga tgacctcgca gtccgcgttg tccgacagga tcgccgccac gttcaggttg
     1321 ttggtgatca cgcgcagccc cttgtggcgc agcagcgcgc gggcgatcgc ctcggtggtg
     1381 gtgccgatgt tgatcagcag cgacgagccg tgcggcacgc gcgccgccac ctcgtgcgcg
     1441 atgcgctgct tggcctgggc gttgagctgc tggcgcttgc ggtactcgat gttctcggtg
     1501 gtcgagcccg gcatgcgcac gccgccatgg aagcgcgtca gcagcccggc gtcgttgagc
     1561 tggcggatgt cgcggcgcac cgtctgcagc gtgacgccga agcgctccgc gagcgcctcg
     1621 acgctgacgg tctgcatgcg gcgcacctcc tcgatgagct cgagctggcg cggagaggga
     1681 ttcatggcga gcgaatctaa acgatcaaat gcgaatcgat tcgagggttt accttgagat
     1741 aaaacgaaaa caaaacgaac agaatcgctg ccaaccgaac tcaacctgaa tgtccgttaa
     1801 gaactcggaa agcaccgtgc aagctgcctc tcccacttcc ggcccgcgct ccggcgcgct
     1861 cccgacccag tgcgacgtcc tcgtcgtcgg gggcgggatc aatggcgcgg gcatcgcgcg
     1921 cgacctcgcc ggccgcggcc tgcaggtggt gctgtgcgag aaggacgacc tggcgcaggc
     1981 gacctcgtcg tccagcacca agctgatcca cggcggcctg cgctacctcg agtacaacga
     2041 gttcgcgctg gtgcgcaagg cgctggccga gcgcgaggtg ctgctgcgca gcgcgccgca
     2101 catcatgtgg ccgcttcgct tcgtgatgcc gcacgacccg ggcatgcggc cggtctggat
     2161 gatccgcatc ggcctgttcc tctacgacca cctggcgcgc cgcgaggtgc tgcccggctc
     2221 gcgcaccgtc gacctgcgcc gccacgccgc gggcgcgccg ctgcagccgc gcttcaccaa
     2281 gggcttcgtc tattccgacg gctgggtgga cgacgcgcgc ctggtggtgc tcaacgccat
     2341 cgacgcgatg gagcgcggcg ccaccatcct cacgcgctgg cactgccgcg acgcgcagcg
     2401 ttcggccgac ggctggcagg cgaccctgga cagcgccgac ggctcgttcc acacgctcaa
     2461 ggcgcgtgcg ctcgtcaacg ccgccgggcc gtgggcggcg cagttcctgt ccgagcacgc
     2521 gcacctgagc cggaacaagt cgctgcggct cgtcaagggc agccacatcg tcgtgcccaa
     2581 gctgttcgag cacggccacg cgtacatctt ccagaacccc gacaagcgca tcatcttcgc
     2641 gatcccgtac gagcacgact tcacgctgat cggcaccacc gacgtcgagc accacggccc
     2701 ggtcggcgcc gcgaggatcg acgaatcgga gatccgctac ctgtgcgagc aggcgagccg
     2761 ctacttcgcc aaggccgtcg tgccggccga cgtggtgtgg acgtactcgg gcgtgcgccc
     2821 gctgctggac gacgagtcgg gcgacccgtc ggccgtcacg cgcgactact cgctggacct
     2881 ggacaccgcg caggccccgc tgctgaccgt gtggggcggc aagatcacca ccttccgccg
     2941 cctcgccgag gacgccgccg acctgctgat ggcgccgctg ggcgtcgatt cccggcgcgg
     3001 ccgctggacg aacggcgcct tcctgcccgg cggcgacctg aacaagtgga tcggccccgc
     3061 gcgccgcccc gacacggact tcgtgcgctt cgaggagacg gtgcgccggc gctacccgca
     3121 gttcgacgcc gcggtgctgc accgcctcgc gcgggcctac ggcgcacgcg tcgaccgcgt
     3181 gctcacggac acgcccggcg ccgaggtcgc gcccggcctg ttcgagtgcg agctggccta
     3241 cctgcacgac cacgaatggg cccgcagcgc cgacgacgtg ctgtggcgtc gcagcaagct
     3301 gggccttcac ctgagcgccg aagagcgcgc ggccgtcgct gcctggtgca ccgagcactg
     3361 ggccggcgcg accgccacat cgaccccgga gaccgcatgg aactgatgct ggaacgcatc
     3421 gaacaacgag cgggcgcgca ggcgcacctg tacccgctcg acctgaagct cgtgcccaac
     3481 gcgatcaccg tgctgctggg cgcgacgcag gccggcaaga ccacgctgat gcgcgtgatg
     3541 gcaggcctgg acaagccgca cgccggccgc gtgctggtgg acggcgcgga cgtgaccggc
     3601 aagccggtgc gcgaccgcaa cgtcgcgatg gtctaccagc agttcatcaa ctacacgtcg
     3661 atgaccgtgg ccgacaacat cgcctcgccg ctgcggctgc gcaaggagac cgacatcgac
     3721 cggcgcgtgc tggaactcgc gcgcaagctg cacatcgagc cgttcctcaa gcgcatgccc
     3781 ggcgagctct cgggcgggca gcagcagcgc gtggcgctcg cgcgggcgct ggccaaggac
     3841 gcgccgctga tgctgttcga cgagccgctg gtcaacctcg actacaagct gcgcgaggaa
     3901 ctgcgcgacg agctctccgc gctgttcgcc gcgggccggt cgaccgtcgt ctacgccacc
     3961 accgagccca ccgaggcgct gctgctgggc ggctacaccg cggtgatgga cgccggcgag
     4021 ctgctgcagt acgggccgac ggccgaggtc ttccatcgcc cgaagtcgat ccgcgtggcg
     4081 cgcgcgttca gcgatccgcc gatgaacctc atcacggggc aggcctcgcc gcagggcgtg
     4141 gcgctgccgg gcggcgcgct cctcgcgtgc acgctgcccc cgggcgcccc gggcgcgctc
     4201 acggtgggcg tgcgcgcggg agcgctgggc gtgcgcgagc ggcccggcga cctcgcgctg
     4261 gcgggcgtcg tggagctggc cgagatctcc ggctccgaca ccttcgtcca cgtgaagacc
     4321 gccgtcggcg agctggtggc gcagctgacc ggcgtgcacg tgttcgcgct cggctcgaac
     4381 gtcactctct acctgcatcc cggccaggcc tacgtgttcg ggcaggacgg caacctcctg
     4441 gtcgcaccga cggagaacca ttgatgtcgc gcatcgacct cgacctggcg cacgcctaca
     4501 agcccgatcc gaagacggac gacgactacg cgctgctgcc gctgaagatg acgttcgacg
     4561 acggcggcgc ctacgcgctg ctgggcccgt cgggctgtgg caagaccacg ctgctcaaca
     4621 tcgtctcggg cctcgtgagg ccctcgcagg gcacggtgaa gtacgacggc aaggacgtca
     4681 ccggcctcac gccgcagcag cgcaacatcg cgcaggtgtt ccagttcccc gtcatctacg
     4741 acacgatgac ggtggccgag aacctcgcgt tcccgctgcg caaccgcggc gtcgcgcgcg
     4801 accggatcaa ggatcgcgtg ggccgcatcg cggagatgct ggagatgagc caccagctcg
     4861 actcgcgcgc cgcgggcctg gccgccgatg ccaagcagaa gatctcgctc gggcgcggcc
     4921 tcgtgcggca ggacgtctcg gccatcctgt tcgacgaacc gctgaccgtg atcgacccgc
     4981 acctcaaatg gca
//
LOCUS       DATCRO010000491         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_500518, whole genome shotgun sequence.
ACCESSION   DATCRO010000491 DATCRO010000000
VERSION     DATCRO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500518"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VIN75_13420"
     CDS             complement(<1..480)
                     /locus_tag="VIN75_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8975216.1"
                     /translation="MLPDLLPSIALFERVAHHGSFTRAAAELGVSPSALSQTLRALEA
                     KLGVRLLERSTRHVRPTEAGQRFLDDARPGLATLASAVEGLDESRSRPAGLLRLNVSQ
                     SAVKAVLQPHLAAFADAFPDIVVELHCDNALLDLVAGGFDAGIRLGENLAEGVVAVPL
                     "
     gene            611..1351
                     /locus_tag="VIN75_13425"
     CDS             611..1351
                     /locus_tag="VIN75_13425"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY8975217.1"
                     /translation="MTSLEGKVAIVTGASKGIGAGIALRLAADGAAVIVNYARAADDA
                     EEVVRRITEAGGRAVAVQADLASPEQVAPLVDAAVRHFGRLDILVNNAGIYGFDRLDD
                     VTPENFDAHFHVNVRGPLLATQAAARVLPAGGVVVNISSGVAKSPSGMTHVYSSTKGA
                     LDVLTRSLAIELGPRGIRVVGVAPGFVETDGNAHLVDDFAPALVAKTPLGRTGRPDDI
                     AATVAFAVSQDAAWVTGTTIDAAGGLVF"
     gene            1578..2798
                     /locus_tag="VIN75_13430"
     CDS             1578..2798
                     /locus_tag="VIN75_13430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8975218.1"
                     /translation="MTDNHKHLRHIAGVLRCTQAIALVVACVAGGALWSEASGFQDAD
                     RWVEHSHEVLEALNDTRVDILRAGVWVRSLSIQPSDHAREQVRRACDQAAAESVRLQE
                     LTLDNPAQQARAKAIVEEVGKTANWIRAAADTGEASGRKALTRLLQTYMESDSTRPLP
                     AELDAMQAEEQRLLRERFDRQGRHLVELKRVAVLTALGLACFLAWSLTYSSRLTRRGH
                     RQIARLAAVSSEDPLTGLLNRRALLLGVDSLREGMSYAVVAFDLDDFKPVNDEHGHGA
                     GDEVLRAVAARLREQCRDQDLLARIGGDEFVAVLPGVCEQRLADIIVTRIRLALGEPI
                     AIEGGEVRIGASLGVAIRGLDGMEFTELLKQADERSYIAKRRAKGLPEIERKHDDAAP
                     AGRAASKPRLTLVG"
BASE COUNT          522 a         1065 c         1126 g          475 t
ORIGIN      
        1 gagcggcact gcgacgacgc cttccgccag gttctcgccc agccggatgc cggcgtcaaa
       61 acctcctgcc acgagatcga gcagcgcgtt gtcgcagtgc agctcgacga cgatgtccgg
      121 gaaggcatcg gcgaacgccg ccaggtgggg ctgcagcact gccttgacgg ccgactggga
      181 gacgttcagg cgcagcaggc ccgccggacg gctgcgcgac tcgtcgagtc cttcgaccgc
      241 cgacgccagc gttgcgaggc cagggcgggc atcgtcgagg aagcgctgcc cggcttccgt
      301 cggccggaca tgccgcgtgg aacgctccag cagccgcacg cccagcttcg cctcgagagc
      361 gcgcaatgtc tgcgacaagg ccgagggcga cacgcccaac tcggcggcag cccgcgtgaa
      421 gctcccatgg tgtgcgacgc gttcgaacag ggcgatcgag ggaagaaggt caggcaacat
      481 gccttggatt atgaagacag gcttcaagcc gagatgagga ttccccggtt tttctttgga
      541 tgtcgagcca ctaccgtgca agccatgccg cgtcgccgat gcggtgcttc acttgagaaa
      601 ggttcctccc atgacatccc tcgaaggcaa ggtcgccatc gtcaccggcg cgtccaaggg
      661 catcggcgcg ggcatcgccc tgcggctcgc cgcggacggc gcagccgtca tcgtcaacta
      721 cgcgagggcg gccgacgacg cggaggaggt cgtgcgccgc atcaccgaag ccggcggccg
      781 tgccgtcgca gtccaggccg acctcgccag tcccgagcag gtcgcgccac tggtcgacgc
      841 ggccgtccgg catttcggcc gcctcgacat cctcgtcaac aacgccggca tctatggctt
      901 cgatcggctg gacgacgtga cgccggagaa cttcgacgcc cacttccatg tcaacgtgcg
      961 cggcccgctg ctcgcgacgc aggccgcggc gcgggtgttg ccggccggcg gcgtggtggt
     1021 caacatcagc tcgggcgttg cgaagagtcc gtcgggcatg acccatgtct actccagcac
     1081 caagggcgcg ctggatgtgc tgacccgctc gctcgccatc gagctcgggc cgcgcggcat
     1141 tcgcgtcgtg ggcgtcgccc cgggcttcgt cgagacggat ggcaacgcac atctcgtgga
     1201 cgacttcgcg ccggcgctgg tcgccaagac accgctgggg cggaccggcc gaccggatga
     1261 catcgccgcg accgtcgcat tcgcggtctc gcaggacgca gcatgggtca cgggaacgac
     1321 gatcgacgcg gcgggggggc tcgtgttctg agccagtcat gtcgtgaagg ccgcctcgcg
     1381 ccacgtcagc tcgacgatgt ggcgcgtcat gcccgggtcg cgggtcgtgc aagggcatcg
     1441 gccgcggcgt ccgtccgggc cggcatcgcc gcgtcgtgac cgggcccacg ccgcgcgtgg
     1501 agatttcccc acacgcgagc gctggagtat cgggggcgtg ccaccgaaac aagcgatgac
     1561 cgcacgagaa ctcacccatg accgacaacc acaagcacct tcgtcacatc gccggggtgc
     1621 tcaggtgcac gcaggcgatc gccctggtcg tcgcctgcgt ggccgggggc gcgctctgga
     1681 gcgaagcatc gggattccag gatgccgacc gatgggtgga gcattcgcac gaagttctcg
     1741 aagccctgaa cgacacccgc gtggacatct tgcgagccgg cgtctgggtg cgcagcctgt
     1801 ccatacagcc cagcgaccat gctcgggagc aggtgcggcg ggcgtgcgac caggcagccg
     1861 cggaatcggt ccgcctgcag gagctgaccc tggacaatcc tgcccagcag gcgcgggcga
     1921 aggccatcgt ggaagaggtc ggcaagacgg ccaactggat cagggccgcg gcggacacgg
     1981 gcgaggcgag cggacgcaag gcgctgaccc ggctgctgca gacctacatg gaaagcgact
     2041 cgacgcggcc actgccggcc gagctcgacg ccatgcaggc cgaggaacaa cggctgctgc
     2101 gtgaacggtt cgaccgccaa ggccggcacc tcgtggaact gaagcgggtg gccgtcctga
     2161 ctgcgctcgg cctggcgtgt ttcctcgcgt ggtcgttgac ctattccagc cgcctgacgc
     2221 gccggggtca caggcagatc gcgcgtctgg ccgccgtgtc ctcggaagac ccgttgaccg
     2281 ggctgctgaa tcgcagggcg ctcctcctgg gcgtggattc cttgcgagaa ggcatgtcgt
     2341 acgccgtggt cgccttcgac ctggacgatt tcaagccggt caatgacgag cacggccatg
     2401 gcgccggcga cgaggtgctc agggcggttg ccgcccgcct gcgcgagcag tgccgcgacc
     2461 aggacctgct ggctcgaatc ggtggcgatg agttcgtcgc agtgcttccc ggcgtctgcg
     2521 agcaacggct ggccgacatc atcgtcacgc gcatccgcct ggccctcggc gagcccattg
     2581 ccatcgaggg tggcgaggtc cggatcggag cgtccctggg ggtcgccatc cggggcctgg
     2641 acggcatgga gttcaccgag ctgctcaagc aggcggacga gcggtcatac atcgccaagc
     2701 ggcgagccaa gggactgccc gaaatcgaga gaaagcacga cgacgctgca cctgcgggcc
     2761 gcgcagcatc caagccgcgc ctgacgctcg tgggctgacg tcgcggggca tcgatacggg
     2821 ctgggcgagc atggcgatcg cgcaggcccg cgcagtccga acacggtcct tcagggccgg
     2881 ccgctgatgg ctggccgacg ggctgaccga gtgcgccggg aaccggcacc gggcatccat
     2941 gcgacgacgg cttgcagccg ccgccgtgat cgaccgctcc ctgccgaccg gggccgcagg
     3001 caacatggca ccattcacac cgttcaggtc cgttcgacgg gtggccggcg aatgaacttt
     3061 tcggggccgg cggtcactga ccctgttgcc gcccggcggc ccggggtcga tggccgggga
     3121 tgcggtgtgg gattccttgg gccgttcaac ggtggtggag tgagattgaa acaagcgctg
     3181 tattgctt
//
LOCUS       DATCRO010000492         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_949979, whole genome shotgun sequence.
ACCESSION   DATCRO010000492 DATCRO010000000
VERSION     DATCRO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949979"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VIN75_13435"
     CDS             <1..528
                     /locus_tag="VIN75_13435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8975219.1"
                     /translation="TERELQRLARVDTLTGLANRRQFDELLEQSLARYRRARRPLALI
                     YLDIDRFKAINDTYGHGIGDAVLREFAARLESGLRETDVAARLSGDEFVVILDGLGTR
                     DEAVGVATKLLHGIRVPMQLAGKALEVTASMGLAYLDGSAEVDAQALMVRADRALYRA
                     KADGRDGLAVADDPA"
     gene            complement(542..1747)
                     /locus_tag="VIN75_13440"
     CDS             complement(542..1747)
                     /locus_tag="VIN75_13440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8975220.1"
                     /translation="MTNDEEESPGVPSRSAHGAWNDTDAQALLGLLLATLRRYGVAFI
                     DLEGRVRGWSEGCHAITGFAADDVLGHSAAVLFTPEDVARELHMHEMNSARLLGHSED
                     ERWHMRKDGSRFWASGITLLLGTTERPLAFAKLFRDATHLRLRMDSLENEVRQMRKER
                     SDRDVFLATIAHELRNPLQPMSVATHLLGEACDQARHGQAVKILDRQLGFIERLVEDL
                     IDMTRLGQGRMAIDLRRVELQRLLQDAADSRNEAARAKGIALACIVPPVPITVEVDPA
                     RFDQVVVNLLNNAIKFTPDGGRVTLLANVDQTHFTVRVQDTGRGISAELQPRLFTMFT
                     QADPVQTGRGQGLGIGLALVKELVSLHHGSVEVRSEGAGRGSEFLVRIPLEQPVAFEG
                     RSDDEPSPA"
     gene            complement(1765..2763)
                     /locus_tag="VIN75_13445"
     CDS             complement(1765..2763)
                     /locus_tag="VIN75_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8975221.1"
                     /translation="MERLIVIGASSGGVAALRTLFQGFGDGLDAPVLVVQHVGRHRSL
                     LPELLSCGDLRVRQASHGERIERGKYLVAPPDRHLLVEGQFVYLQCGPKEHHTRPAID
                     PLFRSAALAFRERVIGVVLTGAMDDGTAGLQAIKSFGGVAVVQDPADAAVPDMPRSAL
                     DHCEVDHCVPLAEMGALLRRLASAPVTPRAEPAAADLQRRIPEHEMALLLDRGDPFEH
                     LNVIGSPSTITCPDCHGVLWAVKGTDPQRYRCHTGHGFSARSLDAGTRVATEDSVWSC
                     VRSLQERRALLESIRDGERREDHRERLATEIEALRKNTEQLRALAGEYLANDAPGR"
BASE COUNT          388 a          989 c         1015 g          417 t
ORIGIN      
        1 accgagcgcg agctgcagcg cctcgcgcgc gtggacacgc tcacgggcct ggccaaccgg
       61 cgccagttcg acgagctgct ggaacagtcg ctggcgcgct accgccgcgc gcgccggccg
      121 ctcgcgctga tctacctgga catcgaccgc ttcaaggcga tcaacgacac ctacggccat
      181 ggcatcggcg acgccgtgct gcgcgagttc gccgcgcggc tggagtcggg actgcgcgag
      241 acggacgtgg cggcgcgcct gtcgggcgac gagttcgtcg tcatcctcga cggcctgggc
      301 acgcgcgacg aggcggtggg cgtggccacc aagctgctgc acggcatccg cgtgccgatg
      361 caactggccg gcaaggcgct ggaggtcacc gccagcatgg gcctggccta cctcgacggc
      421 tcggccgagg tcgatgcgca ggcgttgatg gtgcgggcgg accgggcgct ctaccgagcc
      481 aaggccgacg gccgcgacgg cctggccgtc gccgacgacc cggcctgagc cgggcgcggc
      541 gtcacgcggg cgagggctcg tcgtcgctgc gcccctcgaa ggccaccggc tgttccagcg
      601 ggatgcgcac gaggaattcg ctgccgcggc ccgcaccctc gctgcgcacc tcgaccgacc
      661 cgtggtgcag cgagacgagc tccttgacca gggcgagccc gatgcccagc ccctgtccgc
      721 gtcccgtctg caccggatcg gcctgcgtga acatcgtgaa cagccgcggc tgcagttcgg
      781 cggagatgcc ccggcccgta tcctgcacgc gcacggtgaa gtgcgtctgg tcgacattgg
      841 ccagcagcgt gacgcggccc ccgtcgggcg tgaacttgat ggcgttgttc agcaggttga
      901 cgaccacctg gtcgaaccgt gccggatcga cctcgacggt gatgggcacc ggcggcacga
      961 tgcaggccag cgcgatgccc ttggcgcgcg cggcctcgtt ccgggaatcg gccgcgtcct
     1021 gcagcaggcg ctgcagctcg acgcgtcgca ggtcgatggc catgcggccc tggcccaggc
     1081 gcgtcatgtc gatcaggtcc tccaccagtc gctcgatgaa cccgagctgc cggtcgagga
     1141 tcttgaccgc ctgcccatgt cgtgcctggt cgcacgcctc gccaagcagg tgcgtcgcga
     1201 cgctcatggg ttgcagcgga ttgcgcagct catgcgcgat ggtggcgagg aacacgtcgc
     1261 ggtcgctgcg ctccttgcgc atctgccgca cctcgttctc gaggctgtcc atgcgcaggc
     1321 gcaggtgcgt ggcgtcgcgg aacagtttcg cgaacgccag gggccgttcc gtcgtgccga
     1381 gcagcagggt gatgccgctg gcccagaagc gcgacccgtc cttgcgcatg tgccagcgct
     1441 cgtcctccga atgtccgagc aggcgggcgc tgttcatctc atgcatgtgc agctcgcgtg
     1501 cgacgtcctc cggcgtgaac agcaccgccg ccgagtgccc gagcacgtcg tccgccgcga
     1561 acccggtgat cgcatggcag ccctcgctcc agccgcgcac gcggccctcg agatcgatga
     1621 aggcgacgcc gtagcggcgc agcgtggcca gtagcaggcc cagcagcgcc tgcgcgtccg
     1681 tgtcgttcca ggcgccgtgc gcggatcgac tcggtacgcc gggggactcc tcctcgtcgt
     1741 tcgtcatgtg cggtccgagg ggtgtcagcg cccgggcgcg tcgttggcca ggtactcccc
     1801 cgccagcgcg cgcagttgct cggtattctt gcgcagggcc tcgatctcgg tggccaggcg
     1861 ctcgcggtga tcctcgcgcc gctcaccgtc tcggatgctc tccaggagcg cccgccgttc
     1921 ctgcaggctg cgcacgcagc tccagacgct gtcctcggtg gcgacgcgcg tgccggcgtc
     1981 gagcgagcgc gcgctgaaac cgtggcccgt gtggcagcgg tagcgctgtg gatcggtgcc
     2041 cttgactgcc cacagcacgc catggcaatc gggacaggtg atcgtcgaag gcgacccgat
     2101 gacgttcaga tgctcgaacg ggtcgccgcg gtcgagaagc agggccatct cgtgctccgg
     2161 aatgcgacgt tgcaggtccg cggcggccgg ctcggcccgc ggcgtgacag gggccgacgc
     2221 cagccggcgc agcagcgcgc ccatctcggc gagcggcacg cagtggtcga cttcgcaatg
     2281 gtcgagggcg ctgcgcggca tgtccggcac cgcggcgtcc gccggatcct ggaccacggc
     2341 cacgccgccg aacgacttga tcgcctgcag gccggcggtg ccgtcgtcca tggcgccggt
     2401 gagcacgacg ccgatgacgc gctcgcgaaa ggcgagggcg gccgagcgga acagcgggtc
     2461 gatggcgggg cgggtgtggt gctccttggg gccgcactgg aggtagacga actggccctc
     2521 gaccagcagg tgccggtcgg gcggcgccac caggtacttg ccgcgctcga tgcgttcgcc
     2581 atggctggcc tgacgcacgc gcaggtcgcc gcacgacagc agctccggca gcaggctgcg
     2641 gtgtcttccg acatgctgca ccacgagcac cggcgcgtcc aggccgtcgc cgaagccctg
     2701 gaacagcgtg cgcagcgcgg cgacgccgcc gctcgaggca ccgatcacga tcaggcgttc
     2761 cacggtgctt ctcccggagg gttgtcgtgc gtggaaccgg caagcgggg
//
LOCUS       DATCRO010000493         4608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_61533, whole genome shotgun sequence.
ACCESSION   DATCRO010000493 DATCRO010000000
VERSION     DATCRO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61533"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VIN75_13450"
     CDS             <1..1316
                     /locus_tag="VIN75_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8975222.1"
                     /translation="AAPASPLAKPPVAAASAADTDSDAEDGANLSASARRIYDVGRPR
                     LLQVRTLLRDQDSQASVGSGSLVGPNGLILTNYHVVSQVALEPQRYRLNFQTVDGVTG
                     ALQLIAFDVVHDLALVRMAQPPAAVASRGTFDFRPQATPLAQGERIFSLGNPLDIGFE
                     VIEGTYNGMVERSFLPQILFSGALNPGMSGGPVLDDKGHIVGINVATRRDGQNISFLV
                     PAQFAQALIARGANAKPITGSVEVEMARQLLAHQQSLTTRFIALPWRETKHAHYSVPV
                     PQEDFMRCWGSSNPSESKGLSFERSDCQMDTAIFINESLRTGDIAVRHEVYDGSKLGA
                     LRFAQEAGMSFRNERMGRRTHDLTAPKCQEDFVENAGLPMRTVMCLRAYRHLPGLYDV
                     AVLSQSVDAKTEGVQGRFDARGVSFQNALLLASHYLQGFAWKRSP"
     gene            1298..2305
                     /locus_tag="VIN75_13455"
     CDS             1298..2305
                     /locus_tag="VIN75_13455"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8975223.1"
                     /translation="METLALIETLDRDGLPRQILRVSQWPVRIGRGIDCDVVLDDPHV
                     AAHHATLQMQEDGLHVLPAASLNGVRLGRAAIAPGSAPLLPASALLTLGATTLRVRQA
                     GEALAPEQRLLDLHQYERRHAVTLLLLVVLAALWKGFDLWLASMPGQQGPALAWGYLA
                     APAGLIVWCAGWAIGSMLFQRRFAFWAHLYIALTWLMAGVVVETVTAQLAFAFSMPVL
                     EKLGRIAFVGAMAMMIWRHMGLLLPQRRRAFGIAITSAVVVGGGLLLIDRSVQQQPLV
                     GDLYLGTISLPGMRVARPVTVDAFVKSAAPLEKTLSRWTKSGDGDGDDDDPDGGDDGG
                     E"
     gene            complement(2327..4378)
                     /gene="fusA"
                     /locus_tag="VIN75_13460"
     CDS             complement(2327..4378)
                     /gene="fusA"
                     /locus_tag="VIN75_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8975224.1"
                     /translation="MTILETSDTAAIRNVALVGASATGKTTLTEALLLRAGALKQPGS
                     VERGSTVSDHDPLEKRVGHSLNTAVVNCEHDGIRIQLLDTPGALEFVGQSLPALEAVE
                     TAAIVVSATSGVDSTAVRMMAHAAERKLDRMIIVNRIDVPGARCEAVLAQIREVFGRE
                     CLPLNLPADGGARVVDCFFNRDGQTDFSSPEAAHQALVEQVVEVDADFVDRYLNQGDV
                     EATELHAPLEQALREGHLVPVCFVSARTGAGIDELLDIVTKLLPNPTEGNPPDFLDGA
                     GADAKLMHAVPDAGKHVLAHVFKITVDPYVGKMGVLRVHQGTITRDSQLFVGDGRKPF
                     KVGHLFRLQGGEHVEIARALPGDICAIAKVDALHFDAVLHDAAEDNHIHLKPLQFPVP
                     VHGIAIQPKRHGDEQRLWELVQRAVDEDPCLRIEQVAATHETVVYGLGELHLRMLLDR
                     LREVHKFEVETRPPRTAYRETITAKAEGHHRHKKQTGGAGQFGEVFLRVEPLPRGAGF
                     EFVDIVKGGAIPSNFMAAVEKGVREALAAGVIAGYAVEDIRVTVHDGKSHSVDSKDIA
                     FATAARKATWVAIMEARPIVLEPIVDVEIVAPAAAMGDITGDLASRRGQVNGTEAQLG
                     GMMIVRGQAPAAELIAYATRLNAMTSGEGRYTIAFSHYEPAPATVQNNLRKEHKPVEV
                     D"
BASE COUNT          625 a         1633 c         1649 g          701 t
ORIGIN      
        1 ccgccgcacc ggcctccccg ctggccaagc cgcccgtcgc cgccgcgtcg gccgccgaca
       61 ccgacagcga tgccgaggac ggcgccaacc tgtcggcctc cgcgcgccgc atctacgacg
      121 tcggccggcc acgcctgctg caggtgcgca cgctgctgcg cgaccaggac agccaggcca
      181 gcgtcggctc gggctcgctg gtcgggccca acggactgat cctgaccaac taccacgtgg
      241 tcagccaggt cgcgctcgag ccgcagcgct accggctgaa cttccagacc gtcgacggcg
      301 tcaccggcgc gcttcagctc atcgcgttcg acgtcgtgca cgacctcgcg ctggtgcgca
      361 tggcgcagcc gcccgccgcg gtcgccagcc gcggcacctt cgacttccga ccgcaggcga
      421 cgccgctcgc gcagggcgag cgcatcttct cgctgggcaa tccgctggac atcggcttcg
      481 aggtgatcga gggcacgtac aacggcatgg tggagcgcag cttcctgccg cagatcctgt
      541 tctccggcgc gctgaacccg ggcatgagcg gcgggccggt gctcgacgac aagggccaca
      601 tcgtcggcat caacgtcgcc acgcggcgcg acggccagaa catcagcttc ctggtgcccg
      661 cccagttcgc gcaggcgctg atcgcgcgcg gcgccaacgc caagcccatc acgggctcgg
      721 tggaggtcga gatggcgcgc cagctgctgg cgcaccagca gtcgctcacg acgcgcttca
      781 tcgcgctgcc ctggcgcgag accaagcatg cgcactacag cgtgccggtg ccgcaggaag
      841 acttcatgcg ctgctggggc tcgtcgaacc cgtcggaatc gaagggactg tcgttcgagc
      901 gctcggactg ccagatggac accgccatct tcatcaacga atcgctgcgc acgggcgaca
      961 tcgccgtgcg tcacgaggtc tacgacggca gcaagctcgg tgcgctgcgc ttcgcgcagg
     1021 aggccggcat gagcttccgc aacgagcgca tgggccggcg cacgcacgac ctcaccgcgc
     1081 cgaagtgcca ggaggacttc gtcgagaacg ccggcctgcc gatgcgcacc gtcatgtgcc
     1141 tgcgcgccta tcgtcacctg cccgggctgt acgacgtcgc ggtgctgtcg cagtccgtcg
     1201 acgcgaagac cgagggcgtg cagggccgct tcgacgcgcg cggcgtcagc ttccagaacg
     1261 ccctgctgct cgccagccat tacctgcaag gattcgcatg gaaacgctcg ccctgatcga
     1321 gacgctcgac cgggacggcc tgccgcgcca gatcctgcgc gtgtcgcagt ggccggtgcg
     1381 catcggccgc gggatcgact gcgacgtggt gctcgacgac ccgcacgtgg ccgcgcacca
     1441 cgccacgctc cagatgcaag aggacggcct gcacgtgctg ccggcggcct ctctcaacgg
     1501 cgtgcggctg ggccgcgcgg ccatcgcgcc cggctctgcg ccgctgctgc cggcgtcggc
     1561 gctcctgacg ctgggagcga cgacgctgcg cgtacgccag gccggcgagg cgctggcgcc
     1621 cgagcagcgc ctgctcgacc tgcaccagta cgagcgccgc cacgcggtca cgctgctgct
     1681 cctggtcgtg ctggccgcgt tgtggaaggg cttcgacctg tggctggcct cgatgcccgg
     1741 ccagcagggt cccgcgctcg cctggggcta cctggccgcg ccggcggggc tcatcgtctg
     1801 gtgcgcgggc tgggcgatcg gctcgatgct gttccagcgc cgcttcgcgt tctgggcgca
     1861 cctgtacatc gcgctgacct ggctgatggc cggcgtcgtc gtcgagaccg tcaccgcgca
     1921 gctcgcgttc gcgttctcga tgccggtgct cgagaagctg ggccgcatcg ccttcgtcgg
     1981 cgcgatggcg atgatgatct ggcgccacat gggcctgctg ctgccgcagc gccgccgcgc
     2041 gttcggcatc gcgatcacga gcgccgtcgt ggtgggcggc ggcctgctgc tcatcgaccg
     2101 gtcggtgcag cagcagccgc tggtgggcga cctgtacctg ggcaccatct cgctgccggg
     2161 catgcgcgtg gccaggccgg tgaccgtgga cgccttcgtc aagtcggctg cgccgctcga
     2221 gaagacgctg tcgcgctgga ccaagtccgg cgatggcgac ggcgacgacg acgatccgga
     2281 cggcggggac gacggcggcg agtgaccgcc ccgccgccgg ccgcgttcaa tccacctcga
     2341 cgggcttgtg ctccttgcgc aggttgttct gcaccgtcgc cggcgccggc tcgtagtgcg
     2401 agaacgcgat cgtgtagcgg ccctcgccgc tggtcatcgc attcaggcgc gtcgcatagg
     2461 cgatcagctc cgccgccggc gcctggccgc gcacgatcat catgccgccc agctgcgcct
     2521 cggtgccgtt gacctggccg cggcgtgacg cgaggtcgcc ggtgatgtcg cccatggcgg
     2581 cggccggcgc gacgatctcg acgtcgacga tgggctccag cacgatcggc cgcgcctcca
     2641 tgatcgccac ccacgtggcc ttgcgcgcgg cggtggcgaa cgcgatgtcc ttgctgtcga
     2701 cgctgtggct cttgccgtcg tgcacggtga cgcggatgtc ctcgaccgca tagccggcga
     2761 tgacgcccgc ggccaacgcc tcgcgcacgc ccttctcgac ggccgccatg aagttcgacg
     2821 ggatcgcgcc gcccttgacg atgtcgacga actcgaaccc cgcaccgcgc ggcagcggct
     2881 ccacgcgcag gaacacctcg ccgaactggc cggcgccgcc ggtctgcttc ttgtggcggt
     2941 ggtggccctc ggccttggcg gtgatcgtct cgcggtaggc ggtgcgcggc gggcgcgtct
     3001 cgacctcgaa cttgtgcacc tcgcgcaggc ggtcgagcag catgcgcagg tgcagctcgc
     3061 ccaggccgta gaccaccgtc tcgtgcgtcg ctgccacctg ctcgatgcgc aggcacgggt
     3121 cctcgtcgac cgcccgctgc accagctccc acagccgctg ctcgtcgccg tggcgcttgg
     3181 gctggatcgc gatgccgtgc accggcaccg ggaactgcaa cggcttgagg tggatgtggt
     3241 tgtcctcggc ggcgtcgtgc agcacggcgt cgaagtgcag cgcgtcgacc ttggcgatgg
     3301 cgcagatgtc gcccggcagc gcgcgcgcga tctcgacgtg ctcgccgccc tgcaggcgga
     3361 acaggtggcc gaccttgaac ggcttgcggc cgtcgccgac gaacagctgg ctgtcgcgcg
     3421 tgatcgtgcc ctggtgcacg cgcagcacgc ccatcttgcc gacgtacggg tcgacggtga
     3481 tcttgaacac atgggccagc acgtgcttgc cggcgtcggg cacggcgtgc atgagcttgg
     3541 catcggcgcc ggcgccgtcg aggaagtcgg gcggattgcc ctcggtcggg ttgggcagca
     3601 acttggtgac gatgtccagc agctcgtcga tgcccgcgcc ggtgcgcgcg gacacgaagc
     3661 acacggggac gaggtgcccc tcgcgcagcg cctgctccag cggcgcatgc agctcggtgg
     3721 cctcgacgtc gccctggttg aggtagcggt ccacgaagtc ggcatcgacc tcgaccacct
     3781 gctcgacgag cgcctggtgc gcagcctcgg gcgacgagaa gtcggtctgg ccgtcgcggt
     3841 tgaagaagca gtcgacgacg cgggcgccgc cgtcggcagg caggttcagc ggcaggcatt
     3901 cgcggccgaa gacctcgcgg atctgcgcga gcacggcctc gcagcgggcg ccgggcacgt
     3961 cgatgcggtt gacgatgatc atgcgatcga gcttgcgctc ggccgcgtgg gccatcatgc
     4021 gcacggcggt cgagtcgacc ccggaggtgg cgctgacgac gatggccgcg gtctcgacgg
     4081 cctcgagcgc gggcagcgac tgcccgacga actcgagtgc gccgggcgtg tcgagcagct
     4141 ggatgcggat gccgtcgtgt tcgcagttga cgacggcggt gttcagggag tggccgacgc
     4201 gcttctcgag cgggtcgtgg tcgctgacgg tcgagccgcg ttcgacgctc ccgggctgct
     4261 tgagggcgcc ggcgcgcagc agcagggcct cggtgagtgt ggtcttcccg gtggccgagg
     4321 cgcccaccag ggccacgttg cgaatcgccg cggtatcgga ggtttccagg atcgtcatgg
     4381 cgcgtgtctc cacgggccgt gacgccagtc ggcaccgcgg cgacccgaac cgcgtcacgg
     4441 ccggatggca ggaaccccca gcctacgccg ctcggggcag gcgcgcaatg ggcggcgcgt
     4501 cctggtaagg gcgggttagc cgcaggcgta acccgccgtt cgccggcgcg ctggtcggct
     4561 ctcgggaaac aaaaatgcgg ggcttcgcga cgcaagagga gcggcggg
//
LOCUS       DATCRO010000494         6536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_748120, whole genome shotgun sequence.
ACCESSION   DATCRO010000494 DATCRO010000000
VERSION     DATCRO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748120"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIN75_13465"
     CDS             <1..462
                     /locus_tag="VIN75_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8975225.1"
                     /translation="PAALNLTRYGAINQLFVTDYSGAAPAADLARQYTLLVPKVDANG
                     NETGGIRMPEVAVPVATYAGWNVRAAIRAGGESCGMAGAAIPFAVNPAARSAADPRTT
                     LAQLYTGRADYQARIGAAADALAARGYLLPQDAAAARAGAASISPALIPAP"
     gene            706..1680
                     /gene="dctP"
                     /locus_tag="VIN75_13470"
     CDS             706..1680
                     /gene="dctP"
                     /locus_tag="VIN75_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8975226.1"
                     /translation="MAAPFAGAAFAQEGPLKISHQFPGGSLAEGDFRDRLCRRFAAEI
                     DKRSNGALKAQVYPGSSLMKTNAQFSAMRKGALDLSLVPLSYAGGEVPETNIGLMPAL
                     VPSYEVGSAWKTAEVGKLLSKVLDDKGVLIVSWIWQGGGVASKNAPLVAPADAKGMKV
                     RGGSREMDMMLKEAGASVISLPSNEIYAAMQTGAMEAAMTSSTSFISFKLEEIAKHLT
                     TARNKAYWFMFEPLMISKEVFGKLPKAHQDIIMAVGAEQEKFALEAAKADDKLVAEVY
                     TKAGAKAYDIDDPTLAKWQAIAKATAWKDYADRNETTKSLITAAQKLL"
     gene            1818..2282
                     /locus_tag="VIN75_13475"
     CDS             1818..2282
                     /locus_tag="VIN75_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8975227.1"
                     /translation="MVLGMLALLASALILTSSVVTRYFLHASTDWQDEASVFCLVGAT
                     FLCSAFVQAIRGHIGIEAVSSVLPESANRVRIAIVDIGCLAFCAFFAWKSCTLLHEAW
                     TEGQTTSSSWAPPLWIPYLLMSLGMSLLSVQLALQCVVSVNKLRPSVREGAR"
     gene            2279..3643
                     /locus_tag="VIN75_13480"
     CDS             2279..3643
                     /locus_tag="VIN75_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8975228.1"
                     /translation="MSMVALGLLFGVVTLVIMFSGMPISFSLGAVGVLFMALFMPASS
                     LDTVTQNVYEEMSSITLLSIPLFILKGSAIGRTRAGQDLYAAMHAWMGRIPGGLGIAN
                     VFACALFAAMAGSSPATCSAIGSAGIPEMRRRGYSPGFAAGIIAAGGTLGILLPPSVT
                     MILYAVAAEQSLGRLFLAGIGPGVLLVALFALWAAFQYQREYRAAKHAFDAGGPPSPL
                     LVDEHFTMSQRFAMLPRVLPFLILLTGVMVALYGGYATPSETAGLGSILALVLIALVY
                     GVWRARDVAPILSATLKESTMLMFIIGMSLLFSYVMSYLHISQSMAQWIVGLALSKWV
                     LLAAILLMVIVMGFFLPPVSIILMTAPIFLPPLKAAGFDLVWFGVVMTIVMEAGLIHP
                     PVGLNIFVIKNIAPDIALGDIVRGVIPFVVLMLVTVVVISAFPSIATALPDLVMGPLT
                     RPGG"
     gene            3645..5318
                     /gene="mdcA"
                     /locus_tag="VIN75_13485"
     CDS             3645..5318
                     /gene="mdcA"
                     /locus_tag="VIN75_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEY8975229.1"
                     /translation="MTVIADSPWTRQRDDRAARLARAGSLAGVRGKVADADATIALLE
                     ALIEPGDRVCLEGDNQKQADFLARALAGVRPDRVHDLHMVQSVLSLPEHLDIFESGIA
                     SKLDFSFSGPQSLRLAQLLGQGKLRIGAIHTYLELFGRYFVDLTPKVALVAAQMADRH
                     GNLYTGPNTEDTPAIVEATAFKSGIVVAQVDRIVDLLPRVDIPCDWVDFVVEAPEPAF
                     IEPLFTRDPAQISEIQVLMAMMAIKGIYAEYGVQSLNHGIGFDTAAIELLLPTYGERL
                     GLKGRICRHWALNPHPALIPAIEAGWVESVHSFGSELGMEDYVRARPDVFFTGPDGSL
                     RSNRAFCQAAGHYACDLFIGSTLQMDMAGNSSTATLGRIAGFGGAPNMGSDARGRRHA
                     SQAWLRAGREARGGLPGARGTPRGQKLVVQMVETFREHMQPAFVETLDAWKLAEQAGM
                     PLPPVMIYGDDVTHVLTEEGIANLLLCRDDEEREQAIRGVAGFTPAGRARDRAMVDRL
                     RARGVIRRPSDLGIDPRDATRNLLAARSMRDLVRASGGLYRPPRRFRNW"
     gene            5327..5635
                     /gene="mdcC"
                     /locus_tag="VIN75_13490"
     CDS             5327..5635
                     /gene="mdcC"
                     /locus_tag="VIN75_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase acyl carrier protein"
                     /protein_id="HEY8975230.1"
                     /translation="MESLSFSFDGGRPAGPFAPTLVGVVASGNLEVLVEPSADTGCRI
                     AVDTSARGFAPVWEAVLRDFHERHGLAGLAVAIHDMGATPAVVSLRLDQAIAAIAGGA
                     "
     gene            5635..6534
                     /locus_tag="VIN75_13495"
     CDS             5635..6534
                     /locus_tag="VIN75_13495"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEY8975231.1"
                     /translation="MVSSFAEMSARERLAAVVDAGSFHEWLPPSERLTSPHLAQLGVP
                     AAFDDGVAIGRAALGGRAVFVAAQEGGFMGGGVGEVHGAKLVGLLRRALRDRPDAVVI
                     LAESGGVRLHEANAGLIAVSEVMRALLDVRAAGIATVMLVGGANGCFGGMGIVARCAD
                     TVVMSDIARHAMSGPEVIESAHGVGEFDSRDRALVWRTTGGKHRWLCGDCDVLVDDDA
                     RAFRDAAITALDAHRPMTLEELQRESALLQARADTDGDNADAFELWSRLGVAEPHAVP
                     DLDADALRPRRPAAVAALAQGAA"
BASE COUNT          867 a         2318 c         2338 g         1013 t
ORIGIN      
        1 cccgccgcgc tgaacctgac ccgctacggc gccatcaacc agctgttcgt caccgactac
       61 agcggcgccg cgccggcggc cgacctcgcg cgccagtaca cgctgctggt gcccaaggtc
      121 gacgccaacg gcaacgagac cggcggcatc cgcatgcccg aggtcgccgt gcctgtggcg
      181 acgtatgccg gctggaacgt gcgcgccgcg atccgcgctg gcggcgaaag ctgcggcatg
      241 gccggcgccg ccatcccgtt cgcggtcaac cccgcggccc gctccgccgc tgacccgcgc
      301 accacgctcg cgcagctgta caccggccgc gccgactacc aggcgcggat cggcgccgcg
      361 gccgacgcgc tcgccgcgcg cggctacctg ctgccgcagg acgccgctgc cgcccgcgcc
      421 ggcgccgcgt ccatctcgcc ggcgctgatt ccggcgccct gacgccacgg cgccgcccgc
      481 ggcgcctgct cgactcccgc ccgatccggc gtcgcccgtg cgccgacgca ttcacgcgcg
      541 tccgggttgt ccctagtctt gcgtggtctc attgcatgca aacttcgcca cgttgtattc
      601 ataacggttg acgacgccgc ggcgggcgag ccctgcacgg ccgatcccac aggaggcagc
      661 atggcgatca ctcgacgcac ggtggtgcag gcattcggcg gcgcgatggc cgcgcctttc
      721 gcgggggcgg cgttcgcgca ggaaggcccg ctcaagatct cccaccagtt cccgggcggc
      781 tcgctcgccg agggcgattt ccgggaccgc ctgtgccgcc gcttcgcggc cgagatcgac
      841 aagcgcagca acggcgcgct caaggcgcag gtctatccgg gctcgtcgct gatgaagacg
      901 aacgcgcagt tctcggccat gcgcaagggc gcgctggacc tgagcctggt gccgctgtcg
      961 tacgccggcg gcgaggtgcc cgagaccaac atcggcctca tgcccgcgct cgtgcccagc
     1021 tacgaggtcg gctccgcctg gaagacggcc gaggtcggca agctgctgtc caaggtgctg
     1081 gacgacaagg gcgtgctgat cgtcagctgg atctggcagg gcggcggcgt ggccagcaag
     1141 aacgcgccgc tggtggcccc ggccgacgcc aagggcatga aggtgcgcgg cggcagccgc
     1201 gagatggaca tgatgctcaa ggaggcgggc gcctcggtca tctcgctgcc gtccaacgag
     1261 atctacgccg cgatgcagac cggcgcgatg gaggcggcga tgacgtcgtc gaccagcttc
     1321 atctccttca agctcgagga gatcgcgaaa cacctgacca ccgcgcgcaa caaggcctac
     1381 tggttcatgt tcgagccgct gatgatctcc aaggaggtct tcggcaagct gcccaaggcg
     1441 caccaggaca tcatcatggc cgtcggcgcc gagcaggaga agttcgcgct ggaggccgcg
     1501 aaggcggacg acaagctcgt cgccgaggtc tacacgaagg ccggcgccaa ggcctacgac
     1561 atcgacgacc ccacgctggc caagtggcag gcgatcgcca aggccacggc gtggaaggac
     1621 tacgccgacc gcaacgagac gaccaagtcg ctgatcaccg ccgcgcagaa gctgttgtga
     1681 aagccgacct gccgcacgcg cccgtcgggc tggccgccgc gccggccggc gccgggctgc
     1741 accagcacat cgacgcggcc acgccgcgct ggctgctgcc gctggcgcgc gcgctggtgg
     1801 tcgtcaaccg cggcgtgatg gtgctgggca tgctggcgtt gctggcgtcg gcgctgatcc
     1861 tcacgtccag cgtcgtcacg cgctacttcc tccatgcgtc gaccgactgg caggacgagg
     1921 cctcggtgtt ctgcctggtc ggcgccacgt tcctgtgcag cgcgttcgtg caggcgatcc
     1981 gcggccacat cggcatcgag gcggtgtcgt ccgtcctgcc ggagtccgcg aaccgcgtgc
     2041 gcatcgcgat cgtcgacatc ggctgcctgg cgttctgcgc gttcttcgcg tggaagtcgt
     2101 gcacgctgct gcacgaggcc tggacggaag gccagacgac ctcgtcgtcg tgggcgccgc
     2161 cgttgtggat cccgtacctg ctgatgtcgc tgggcatgtc cctgctgagc gtgcagctgg
     2221 cgctgcagtg cgtcgtcagc gtcaacaagc tgcggccgtc cgtgcgcgag ggcgcgcgat
     2281 gagcatggtg gcgctcggcc tgctgttcgg cgtggtcacg ctggtgatca tgttctcggg
     2341 catgcccatc tcgttctcgc tgggcgcggt gggcgtgctg ttcatggcgc tgttcatgcc
     2401 ggcctcgtcg ctggacacgg tcacgcagaa cgtctacgag gagatgtcca gcatcacgct
     2461 gctgtcgatc ccgctgttca tcctcaaggg ttcggcgatc ggtcgcacgc gcgccggcca
     2521 ggacctgtac gcggcgatgc acgcgtggat gggccgcatc cccggcggcc tgggcatcgc
     2581 caacgtgttc gcctgcgcac tgttcgccgc gatggccggc tcgagcccgg ccacgtgctc
     2641 ggcgatcggc agcgccggca tccccgagat gcgccgccgc ggctactcgc cgggcttcgc
     2701 ggcgggcatc atcgccgcgg gcggcacgct gggcatcctg ctgccgccgt cggtcacgat
     2761 gatcctgtat gccgtggccg cggagcagtc gctgggccgc ctgttcctcg cgggcatcgg
     2821 gccgggcgtg ctgctcgtgg cgctgttcgc gctgtgggcc gcgttccagt accagcgcga
     2881 ataccgggcc gcgaaacacg cgttcgacgc cggcggcccg ccatcgccgc tgctggtgga
     2941 cgagcacttc acgatgagcc agcgcttcgc gatgctgccg cgcgtgctgc cgttcctgat
     3001 cctgctgacc ggcgtgatgg tggcgctgta cggcggctat gccacgccgt ccgagacggc
     3061 cggcctcggc tcgatcctcg cgctggtgct gatcgcgctg gtctacggcg tctggcgcgc
     3121 gcgcgacgtc gcgcccatcc tgtcggccac gctcaaggag tcgacgatgc tgatgttcat
     3181 catcggcatg tcgctgctgt tctcctacgt gatgagctac cttcacatca gccagtcgat
     3241 ggcgcagtgg atcgtcgggc tcgcgctgtc gaagtgggtg ctgctcgcgg cgatcctgct
     3301 gatggtgatc gtgatgggct tcttcctgcc gccggtctcc atcatcctga tgaccgcgcc
     3361 gatcttcctg ccgccgctca aggccgcggg cttcgacctg gtgtggttcg gcgtggtgat
     3421 gaccatcgtg atggaggccg gcctgatcca cccgccggtg ggcctgaaca tcttcgtcat
     3481 caagaacatc gcgcccgaca tcgcgctggg cgacatcgtc cgcggcgtga tcccgttcgt
     3541 cgtgctgatg ctggtgacgg tggtcgtgat ctcggcgttc ccgtcgatcg ccaccgcgct
     3601 gcccgacctc gtgatggggc cgctgacgcg accgggaggc tgacgtgacg gtcatcgccg
     3661 attcgccctg gacgcgccag cgcgacgacc gcgcggcccg gctcgcgcgc gcgggctcgc
     3721 tcgcgggcgt gcgaggcaag gtggccgacg cggacgcgac gatcgcgctg ctcgaggcgc
     3781 tgatcgagcc gggcgaccgc gtctgcctgg agggcgacaa ccagaagcag gccgacttcc
     3841 tcgcacgcgc gctcgcgggc gtcaggcccg accgcgtgca cgacctgcac atggtgcagt
     3901 cggtgctgtc gctgcccgag cacctggaca tcttcgagtc cggcatcgcc agcaagctcg
     3961 acttctcgtt ctccggcccg cagtcgctgc ggctcgcgca gctgctgggg cagggcaagc
     4021 tcaggatcgg cgcgatccac acctacctgg agctgttcgg ccgttacttc gtcgacctga
     4081 cgcccaaggt ggcgctggtc gccgcgcaga tggccgaccg ccacggcaac ctctacacgg
     4141 gcccgaacac cgaggacacg ccggccatcg tcgaggccac ggcgttcaag agcggcatcg
     4201 tcgtcgcgca ggtcgaccga atcgtcgacc tgctgccgcg ggtggacatc ccttgcgact
     4261 gggtcgactt cgtggtcgag gcgcccgagc ccgcgttcat cgagccgctg ttcacgcgcg
     4321 acccggcgca gatctcggag atccaggtgc tgatggcgat gatggccatc aagggcatct
     4381 acgcggaata cggcgtgcag tcgctcaacc acggcatcgg cttcgacacc gcggccatcg
     4441 aactgctgct gccaacctac ggcgaacgcc tcgggctgaa aggcaggatc tgccgccact
     4501 gggcgctcaa tccgcatccc gcgctgatcc cggcgatcga ggccggctgg gtcgagtcgg
     4561 tgcactcgtt cggctccgag ctgggcatgg aggactacgt gcgtgcccgg cccgacgtct
     4621 tcttcaccgg ccccgacggc agcctgcgca gcaatcgcgc cttctgccag gccgcgggcc
     4681 actacgcgtg cgacctgttc atcggctcga cgctgcagat ggacatggcc ggcaacagct
     4741 cgaccgccac gctgggccgc atcgccggct tcggcggcgc gcccaacatg ggctcggacg
     4801 cgcgcggccg ccgccacgcg agccaggcct ggctgcgcgc cggccgcgag gcgcgcggcg
     4861 ggctgcccgg cgcgcgcggc acgccgcgcg ggcagaagct ggtggtgcag atggtggaga
     4921 ccttccgcga gcacatgcag cccgcgttcg tcgagacgct ggatgcctgg aagctggccg
     4981 agcaggccgg catgccgctg ccgccggtga tgatctacgg cgacgacgtg acgcacgtgc
     5041 tgacggagga aggcatcgcc aacctgctgc tgtgccgcga cgacgaggaa cgcgagcagg
     5101 cgatccgcgg cgtcgccggc ttcacgcccg cgggccgcgc acgcgatcgc gcgatggtcg
     5161 acaggctgcg tgcgcgcggc gtcatccgcc gtccgtcgga cctgggcatc gacccgcgcg
     5221 acgcgacgcg caacctgctg gccgcgcgct cgatgcgcga cctggtgcgc gcgtcgggcg
     5281 ggctgtaccg gccgccgcgc cggttccgca actggtgagc ccgctcatgg aatccctgag
     5341 cttctcgttc gacggcggcc gcccggcggg gccgttcgcg ccgacgctcg tgggcgtggt
     5401 ggcgtccggc aatctcgagg tcctggtcga gccgtcggcc gatacgggtt gccgtatcgc
     5461 cgtcgatacc tccgcgcgcg gcttcgcgcc ggtgtgggag gcggtgctgc gcgacttcca
     5521 cgagcgccac ggcctcgccg gcctcgcggt cgcgatccac gacatgggcg ccacgccggc
     5581 cgtggtgagc ctgcggctgg accaggcgat cgccgcgatc gcgggaggcg cgtgatggtc
     5641 tcgagcttcg ccgagatgtc ggcgcgcgag cgcctggccg cggtcgtcga cgccggcagt
     5701 ttccacgaat ggctgccgcc gtccgagcgc ttgacgagcc cgcacctggc gcagctgggc
     5761 gtgcccgcgg cgttcgacga cggcgtggcg atcggccgtg ccgcgctcgg cggccgcgcg
     5821 gtgttcgtcg ccgcgcagga gggcggcttc atgggcggcg gcgtcggcga ggtgcacggc
     5881 gccaagctgg tgggcctgct gcggcgcgcg ctgcgcgacc ggcccgacgc ggtggtgatc
     5941 ctcgccgagt ccggcggcgt gcggctgcac gaggccaatg cggggctgat cgcggtgtcc
     6001 gaggtgatgc gcgcgctgct cgacgtgcgt gccgccggca tcgccacggt gatgctggtc
     6061 ggcggggcca acggctgctt cggcggcatg ggcatcgtcg cgcgctgcgc cgacacggtc
     6121 gtcatgagcg acatcgcgcg ccatgcgatg tcggggcccg aggtgatcga gtccgcgcac
     6181 ggcgtcggcg agttcgactc gcgcgaccgc gcgctcgtgt ggcgcaccac cggcggcaag
     6241 caccgctggc tgtgcggcga ctgcgacgtg ctggtcgacg acgacgcgcg cgcgttccgc
     6301 gacgccgcga tcaccgcgct ggatgcgcat cgaccgatga cgctcgagga gctccagcgg
     6361 gagtcggcgc tgctgcaggc gcgcgccgac acggacggcg acaacgccga cgcgttcgag
     6421 ctgtggtcgc gcctgggcgt cgccgagccg catgcggtgc ccgatctcga cgccgacgcg
     6481 ctgcggccgc gccgcccggc cgcggtggcc gcgctcgcgc aaggagccgc gtgatg
//
LOCUS       DATCRO010000495         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_151740, whole genome shotgun sequence.
ACCESSION   DATCRO010000495 DATCRO010000000
VERSION     DATCRO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151740"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VIN75_13500"
     CDS             complement(<1..687)
                     /locus_tag="VIN75_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY8975232.1"
                     /translation="MSKQISDAYIVAATRTPIGKSHRGYFKNTRPDDLLVAALQGALK
                     QVPNLDPKSIEDAIVGCAMPEGEQGLNFARTSVLLAGLPNSVGGITVNRFCASGLSAV
                     QMAADRIRVGEADVMIAAGAESMSMVPMSGNKPSFNPHVFDKDENIGIAYGMGLTAEK
                     VANQWKVSREAQDAFALASHQKALAAIAAGQFKDEMTPVEVFTRTPNLATGEIDVTSR
                     WVDIDEGPRPD"
     gene            complement(702..3083)
                     /locus_tag="VIN75_13505"
     CDS             complement(702..3083)
                     /locus_tag="VIN75_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8975233.1"
                     /translation="MSRFQVRKVAVLGAGVMGAQIAAHLVNVKVPVVLFDLPAKSGPK
                     NGIVTKAVDGLKKLKPAPLGVADDAALIQQANYEDHLELLRDCDLVIEAIAERMDWKL
                     DLYTKVAPFIAPHAIVASNTSGLSITKLSEVLPEEIKPRFCGIHFFNPPRYMRLVELI
                     PTPTTQPQILDQLETFVTTALGKGVVRAKDTPNFVANRVGIAGMLATIIEAEKFGLSY
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTMAHVIKTLQDNLKDDPFFPSYATPPVVGR
                     LIEQGALGQKAGAGFYKKVGKDILRLDPSKGEYVPGGAKADAIVDRILKKPAAERLKL
                     LRESTNPQAQFLWAILRDGFHYAAVHLADIADSAREIDFAMRWGFGTKQGPFELWQDA
                     GWKQVAEWIKEDIAAGKALSTAPLPAWVFDGRDGVHTSDGSWSASQGKYLAPSQLDVY
                     KRQPFPETVLGAGAANPLKSGTEEFRNDEVRVWTLDGEVLVASITAKLHLISPTVTEG
                     LLKAVEIAEAKYKGLVIWSPDEAFSAGANLEALMPVFMKMGAKGIAPEEKKLQDMMLR
                     IRYAAVPVVAAVRGIALGGGCELAVHCASRVAAMESYMGLVEVGVGLIPGGGGLTYIA
                     RRAAENAAKAPGTDLLAFLKDGVVAAASATVGTSALESRKIGYLLDSDIIVPHIDELL
                     HVASTQAKAMYDAGYRAPVKRLIPVAGRSGIATVKASLVAMRDGGFISQHDFFIGSLI
                     ADVCCGGEVDAGTPVTEEYLMALERKHFCALLENPKTQERIMGMLQTGKPVRN"
     gene            complement(3160..>3366)
                     /locus_tag="VIN75_13510"
     CDS             complement(3160..>3366)
                     /locus_tag="VIN75_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY8975234.1"
                     /translation="IVGMTIINGVKQDAEEPGLWDGGLILDPAKGSTYKVRLKTVDGG
                     KKLEVRGYIGSPMFGRTQTWIRAE"
BASE COUNT          472 a         1142 c         1159 g          593 t
ORIGIN      
        1 gtcgggacgc gggccttcgt cgatgtcgac ccagcggctc gtgacgtcga tctcgcccgt
       61 ggccaggttc ggcgtgcgcg tgaacacctc gacgggcgtc atctcgtcct tgaactggcc
      121 ggcggcgatg gcggcgagcg ccttctggtg cgaggccagc gcgaacgcgt cctgcgcctc
      181 gcgcgagacc ttccactggt tggcgacctt ctcggccgtc aggcccatgc cgtaggcgat
      241 gccgatgttc tcgtccttgt cgaacacgtg cgggttgaac gacggcttgt tgccgctcat
      301 gggcaccatg ctcatgctct cggcgccggc ggcgatcatc acgtcggcct cgcccacgcg
      361 gatgcggtcg gccgccatct gcacggccga caggccggac gcgcagaagc ggttgaccgt
      421 gatgccgccc acggaattgg gcaggccggc cagcagcacg ctggttcgcg cgaagttcag
      481 gccctgctcg ccctcgggca tcgcgcagcc gacgatcgcg tcctcgatgg atttcggatc
      541 gaggttgggc acctgcttga gcgcaccctg cagcgccgcg accagcaggt cgtccggacg
      601 cgtgttcttg aagtagccgc gatgcgactt gccgatcggc gtgcgggtgg ccgcgacgat
      661 gtaggcgtcg gaaatttgct tgctcatagt cgtgcgtcca atcagttccg cacgggcttg
      721 ccggtctgca gcatgcccat gattcgttct tgcgtcttcg ggttctcgag cagcgcgcag
      781 aagtgcttgc gctcgagcgc catcaggtat tcctcggtca cgggcgtgcc cgcgtcgacc
      841 tcgccgccgc agcagacgtc ggcgatcagg ctgccgatga agaagtcgtg ctggctgatg
      901 aagccgccgt cgcgcatcgc gaccagcgag gccttgaccg tggcgatgcc cgagcggccc
      961 gccaccggga tcaggcgctt gaccggcgcg cggtagcccg cgtcgtacat cgccttggcc
     1021 tgcgtcgagg ccacgtgcag cagctcgtcg atgtgcggga cgatgatgtc cgaatcgagc
     1081 aggtaaccga tcttgcgcga ctcgagcgcg ctcgtgccca ccgtggccga ggccgcggcg
     1141 accacgccgt ccttcaggaa ggccagcagg tcggtgcccg gcgccttggc cgcgttctcc
     1201 gcggcgcggc gcgcgatgta ggtcagcccg ccgccgcccg ggatcaggcc gacgcccact
     1261 tccaccaggc ccatgtagct ttccatcgcg gccacgcgcg aggcgcagtg cacggccagc
     1321 tcgcagccgc cgcccagcgc gatgccacgg accgcggcga ccaccggaac ggcggcgtag
     1381 cggatgcgca gcatcatgtc ctgcagcttc ttctcttccg gcgcgatgcc cttggcgccc
     1441 atcttcatga agaccggcat cagggcctcg aggttggcgc cggcggagaa cgcctcgtcc
     1501 ggcgaccaga tcaccaggcc cttgtacttc gcctcggcga tctcgacggc cttgagcagg
     1561 ccctcggtga ccgtcggcga gatcaggtgc agcttggcgg tgatgctggc gaccagcacc
     1621 tcgccgtcca gcgtccacac gcgcacctcg tcgttcctga actcttccgt gcccgacttg
     1681 agcgggttgg ccgcgccggc gcccagcacc gtctcgggga agggctggcg cttgtagacg
     1741 tccagctgcg acggcgcgag gtacttgccc tgcgacgcgc tccacgagcc gtccgaggtg
     1801 tgcacgccgt cgcggccgtc gaacacccag gcgggcagcg gcgcggtcga cagcgccttg
     1861 ccggcggcga tgtcttcctt gatccactcg gcgacctgct tccagccggc gtcctgccac
     1921 agctcgaacg ggccctgctt ggtgccgaag ccccagcgca tcgcgaagtc gatctcgcgc
     1981 gccgagtcgg cgatgtcggc caggtgcacg gcggcgtagt ggaagccgtc gcgcaggatg
     2041 gcccacagga actgcgcctg cgggttggtc gattcacgca gcagcttgag gcgctcggcc
     2101 gcgggcttct tcaggatgcg gtcgacgatc gcgtcggcct tggctccgcc cgggacgtac
     2161 tcgcccttgg acggatccag gcgcaggatg tccttgccga ccttcttgta gaagccggcg
     2221 ccggccttct ggcccagcgc gccctgctcg atcaggcggc cgacgaccgg cggcgtggcg
     2281 tagctcggga agaacgggtc gtccttgagg ttgtcctgca gcgtcttgat gacgtgggcc
     2341 atcgtgtcca ggccgacgac gtccgcggtg cggaacgtgc ccgagctcgc gcggcccagc
     2401 ttcttgccgg tcaggtcgtc gacgacgtcg tacgacaggc cgaacttctc ggcctcgatg
     2461 atcgtggcca gcatgccggc gatgccgacg cggttggcga cgaagttggg cgtgtccttg
     2521 gcgcgcacga cgcccttgcc cagcgcggtg gtgacgaagg tctcgagctg gtcgaggatc
     2581 tgcggctgcg tcgtcggcgt ggggatcagc tccaccaggc gcatgtagcg cggcgggttg
     2641 aagaagtgga tgccgcagaa gcgcggcttg atctcttcgg gcagcacttc ggacagcttg
     2701 gtgatcgaca ggcccgacgt gttggacgcg acgatcgcgt gcggggcgat gaacggcgcg
     2761 accttggtgt acaggtccag cttccagtcc atgcgctcgg cgatcgcctc gatcaccagg
     2821 tcgcagtcgc gcagcagctc gaggtggtcc tcgtagttgg cctgctggat cagcgcggcg
     2881 tcgtcggcca cgcccagcgg cgcgggcttg agcttcttga ggccgtcgac ggccttcgtg
     2941 acgatcccgt tcttggggcc ggacttggcc ggcaggtcga acaggacgac gggcaccttg
     3001 acgttgacca gatgcgccgc gatctgcgcg cccatcacgc cggcgccgag cacggccacc
     3061 ttgcgaactt ggaatcgact cactttgttt ctctccaggg gtgaacgggg tcggccggca
     3121 gcgcgctgct gcccggcccc gggatcaacg gacctgcgct tattcggcgc ggatccaggt
     3181 ctgcgtgcga ccgaacatcg gcgagccgat gtagccgcgc acttccagct tcttgccgcc
     3241 gtcgacggtc ttcaggcgga ccttgtacgt gctgcccttg gccggatcga ggatgagacc
     3301 gccgtcccac agaccgggct cctcggcatc ctgcttgacg ccgttgatga tcgtcatgcc
     3361 gacgat
//
LOCUS       DATCRO010000496         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_106517, whole genome shotgun sequence.
ACCESSION   DATCRO010000496 DATCRO010000000
VERSION     DATCRO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106517"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VIN75_13515"
     CDS             complement(<1..1236)
                     /locus_tag="VIN75_13515"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY8975235.1"
                     /translation="MTLPSDTHALPAPDAVPHCDAPAPGAHDCGHAAAATTRQPLPAS
                     RKVRVQGLQPGVSVPMREIALTNGEKVTVYDTSGPYTDPTALIDLRRGLETVRSRWIA
                     ARNDSESYAGRDAQPVDDGQRPRAPSAGTAATRIATLRREADGLQRTPRRAVPGRNVT
                     QMHYARRGIVTPEMEFVALRENGRHEWMAEYQANAQREMRLRGDPRGAAIPARITPEF
                     VRDEVARGRAIIPANINHPELEPMIIGRNFLVKVNANIGNSATTSSIEEEVDKLVWAT
                     RWGADTVMDLSTGANIHTTRDWILRNSPVPIGTVPIYQALEKVGGVAEDLSWPLFRDT
                     LIEQAEQGVDYFTIHAGVLAEFVPLTAHRRTGIVSRGGAILAKWCMAHGCQNFLFTHF
                     EEICEIMKAYDVSFSLGDGL"
     regulatory      complement(1320..1440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1581..2825
                     /locus_tag="VIN75_13520"
     CDS             1581..2825
                     /locus_tag="VIN75_13520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8975236.1"
                     /translation="MASIARPASPSPVRHPYWILAAVCLLGLMSTVGVSMPYPILAPI
                     FIGGTADDFTHFGGLQPSVLMGIALAANPLGILIGSLFSGPLSDRHGRRVVLAVTLVA
                     TLAGHLLTAFALVVRDYPLFALARFATGLVESNTAVARAMLADMHEQIDRTRAFALLN
                     ACFYAGWLLGPLVGGLTLPLGEPVPFVLAAVMTLPCVAILFLGLPSAAPRPAGGLDEA
                     LQHRNVLALLRADRALGVIVGLQLAYTLALNTLYEFSPMWMLKNAGFDSRGIALVTAG
                     QCAAMTLASVAAGRFGGTPGRHPLRRAARLALVAALCLSLMAVLPGWLGICVIMAMGV
                     PTALYNAVMPAWMSERFAGHGQGRIMGLLSTIFCVANVIVALAGGWIALLSTRWIMAL
                     GGAAAVVSALLMLRLARREEAR"
     gene            2904..3950
                     /locus_tag="VIN75_13525"
     CDS             2904..3950
                     /locus_tag="VIN75_13525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY8975237.1"
                     /translation="MTTTTARAVGWVEQGLVPDRVVRLGIRRLLRERLAEMHADDAEA
                     AEEAGQAFVDAMRRAPVALLPEKANEQHYEVPAAFFARVLGPHRKYSCCWWGEGVETL
                     AQAEAAALALTCERAGLRDGQDVLELGCGWGSLSLWMAERHPASRITAVSNSNSQRRF
                     IEDAALARGLSNLRVVTCDANQFDAGPGHFDRVVSVEMFEHLRNWPEMFARVARWLKA
                     DGRFFMHVFVTRGAPYAFEERDASDWMSRHFFSGGMMPADGLALHFQDDLRVLQRWRW
                     DGTHYQRTAEAWLARMDESRDELMPLMAATYGVAAETWWQRWRLFFMSVAELFGHEGG
                     QQWWVSHYLFEKRA"
     gene            3956..>4284
                     /locus_tag="VIN75_13530"
     CDS             3956..>4284
                     /locus_tag="VIN75_13530"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 family protein"
                     /protein_id="HEY8975238.1"
                     /translation="MHALPRAGGRAARDARGPSRGRMLANLAVFEAAWFACLLAVAHG
                     RPAWGTAAVAAAVAWHVAISARPATELALVGALCAIGLVAETTMVALGYVAYPCGQPV
                     AWLAPYWL"
BASE COUNT          499 a         1485 c         1590 g          710 t
ORIGIN      
        1 caggccgtcg cccagcgaga acgagacgtc gtacgccttc atgatctcgc agatctcctc
       61 gaagtgcgtg aacaggaagt tctggcagcc gtgcgccatg caccacttgg ccaggatcgc
      121 gccgccgcgc gacacgatgc ccgtgcggcg atgcgccgtc agcggcacga actcggccag
      181 cacgccggcg tggatggtga agtagtcgac gccctgctcg gcctgctcga tcagcgtgtc
      241 gcggaacagc ggccagctca ggtcctcggc gacgccgccc accttctcga gcgcctggta
      301 gatcggcacg gtgccgatgg gcaccgggct gttgcgcagg atccagtcgc gcgtggtgtg
      361 gatgttggcg cctgtggaca ggtccatcac cgtgtccgcg ccccagcgcg tggcccacac
      421 gagcttgtcg acctcctcct cgatggacga ggtggtcgcc gagttgccga tgttggcgtt
      481 gaccttcacc aggaagttgc ggccgatgat catcggctcg agctccgggt ggttgatgtt
      541 ggccgggatg atggcgcgtc cgcgcgccac ctcgtcgcgc acgaactcgg gcgtgatgcg
      601 cgccgggatc gcggcgccgc gcgggtcgcc gcgcaggcgc atctcgcgct gcgcgttggc
      661 ctggtattcg gccatccact cgtggcggcc gttctcgcgc agggccacga actccatctc
      721 gggcgtgacg atgccgcgcc tggcgtagtg catctgcgtc acgttgcgtc ccggcacggc
      781 gcggcgcggc gtgcgctgca ggccgtcggc ctcgcgccgc agcgtggcga tccgcgtggc
      841 cgcggtgccg gccgagggcg cgcgcgggcg ctggccgtcg tccaccggct gcgcgtcgcg
      901 tccggcgtag gactcgctgt cgttgcgtgc ggcgatccag cgcgagcgca ccgtttccag
      961 cccgcgcctc aggtcgatca gcgcggtcgg gtcggtgtac gggccggagg tgtcgtagac
     1021 ggtgaccttc tcgccgttgg tcagcgcgat ctcgcgcatg ggcacgctga ccccgggctg
     1081 cagcccctgc acgcgcacct tgcgcgaggc gggcagcggc tggcgcgtgg tcgcggcggc
     1141 ggcgtggccg cagtcgtggg cgcccggcgc gggtgcgtcg cagtggggga cggcgtcggg
     1201 cgcgggcagc gcgtgggtgt cggaaggcaa ggtcatcgag gctccagtcc tggggtcaag
     1261 tcttcttgct ccggacggtg cgccgacctt gcgcgcgggc gcctcgtgga cgcccggatg
     1321 cgggttcgtg cagctctcct tacgccggta ctaaccggtt caagttctcg ggttcgacct
     1381 agattaccca tccaatggtc gtctcagcat ctcttggcgg atgcaccccg gagcgcgttg
     1441 cagtttacga cggccgcgtg gggatgcaat gacttttttc gggtattcca agtcgctggt
     1501 tagacacccc agggggtatc gaatagtttg acttttgacc acccggcctg gccgcctaga
     1561 cagagggccg acaatgccgc atggcatcga tcgcccgccc ggcttcgccg tcccccgtcc
     1621 gccacccgta ctggatcctc gccgccgtct gcctgctggg gctcatgagc acggtgggcg
     1681 tgtcgatgcc gtatccgatc ctggcgccga tcttcatcgg cggcacggcc gacgatttca
     1741 cgcacttcgg ggggctgcag ccgtccgtgc tcatgggcat cgcgctggca gccaatccgc
     1801 tgggcatcct gatcggcagc ctgttctcgg ggccgctgtc ggatcgccac gggcgccgcg
     1861 tggtgctggc ggtgacgctg gtggccacgc tcgccggcca cctgctgacc gcgttcgcgc
     1921 tggtcgtgcg cgactatccg ctgttcgcgc tggcgcgctt cgccacgggg ctggtcgaga
     1981 gcaacaccgc cgtcgcgcgc gcgatgctgg ccgacatgca cgagcagatc gaccgcaccc
     2041 gcgcgttcgc gctgctgaac gcctgcttct acgccggctg gctgctcggg ccgctggtgg
     2101 gcggcctcac gctgccgctg ggcgagcccg tgcccttcgt gctggccgcg gtgatgacgc
     2161 tgccctgcgt ggcgatcctg ttcctcgggc tgccgtcggc ggcgcccagg ccggcgggtg
     2221 ggctggacga ggcgctgcag catcgcaacg tgctggcgct gctgcgcgcc gatcgcgcgc
     2281 tgggcgtgat cgtcggcctg cagctggcct acacgctggc gctgaacacg ctctacgagt
     2341 tctcgccgat gtggatgctc aagaacgccg gcttcgacag ccgcggcatc gcgctcgtca
     2401 ccgccggcca gtgcgccgcg atgacgctgg ccagcgtggc ggccggccgc ttcggcggca
     2461 cgcccggacg gcacccgctg cgccgcgccg cgcgcctggc gctggtggcg gcgctgtgcc
     2521 tgagcctgat ggcggtgctg cccggctggc tcggcatctg cgtgatcatg gcgatgggcg
     2581 tgccgaccgc gctgtacaac gcggtgatgc cggcgtggat gtccgagcgc ttcgccgggc
     2641 atggccaggg ccgcatcatg ggcctgctgt cgacgatctt ctgcgtcgcc aacgtgatcg
     2701 tggcgctggc cggcggctgg atcgcgctgc tgtccacgcg ctggatcatg gcgctcggcg
     2761 gcgcggcggc ggtggtgtcg gcgctgctga tgctgcggct ggcgcggcgc gaggaagcgc
     2821 gctgaccgct gccgggaggt gaatccggat cgcccggctc tccgtatccg gaagatcgaa
     2881 gcgccgtcac gaggagttgc cgcatgacca ccaccaccgc ccgcgccgtc ggctgggtcg
     2941 agcagggcct ggtgcccgat cgcgtcgtcc ggctgggcat ccgccgcctg ctgcgcgagc
     3001 gcctggccga gatgcacgcg gacgacgccg aggccgccga ggaggccggc caggccttcg
     3061 tcgacgcgat gcggcgcgcg ccggtggcgc tgctgcccga gaaggccaac gagcagcact
     3121 acgaggtgcc ggccgccttc ttcgcgcgcg tgctgggccc gcaccgcaag tacagctgct
     3181 gctggtgggg cgagggcgtc gagacgctcg cccaggccga ggccgccgcg ctggcgctca
     3241 cctgcgagcg cgcgggcctg cgcgacgggc aggacgtgct cgagctgggt tgcggctggg
     3301 gctcgctgag cctgtggatg gccgagcgcc atccggccag ccgcatcacg gcggtgtcca
     3361 actcgaactc gcagcgccgc ttcatcgagg acgcggcgct ggcgcgcgga ctgtccaacc
     3421 tgcgcgtcgt cacctgcgac gccaaccagt tcgacgccgg ccccggccac ttcgaccgcg
     3481 tcgtctcggt ggagatgttc gagcacctgc gcaactggcc cgagatgttc gcccgggtcg
     3541 cgcgctggct caaggccgac ggccgcttct tcatgcacgt gttcgtcacg cgcggagcgc
     3601 cgtatgcgtt cgaggagcgc gatgccagcg actggatgag ccggcacttc ttctccggcg
     3661 gcatgatgcc cgccgacggc ctcgcgctgc atttccagga cgacctgcgc gtgctgcagc
     3721 gctggcgctg ggacggcacg cactaccagc gcaccgccga ggcctggctc gcgcgcatgg
     3781 acgagtcgcg cgacgagctc atgccgctga tggccgccac ctacggcgtg gccgccgaga
     3841 cctggtggca gcgctggcgc ctgttcttca tgtcggtggc cgagctgttc ggccacgagg
     3901 gcgggcagca atggtgggtg agccactatc ttttcgagaa gcgcgcgtga acgccatgca
     3961 cgcgctgccg cgggcgggcg ggcgcgccgc gcgtgacgcg cgcgggccct cgcgcgggcg
     4021 catgctcgcg aacctggccg tgttcgaggc ggcgtggttc gcgtgccttc tggccgtcgc
     4081 ccacggccgg cccgcgtggg gcacggccgc cgtggccgcg gccgtcgcct ggcacgtggc
     4141 gatctccgcg cggccggcga ccgagctcgc gctggtgggc gcgctgtgcg ccatcggcct
     4201 ggtggccgag acgacgatgg tcgcgctcgg gtacgtggcg tatccgtgcg gccagccggt
     4261 ggcgtggctg gcgccgtact ggct
//
LOCUS       DATCRO010000497         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_208053, whole genome shotgun sequence.
ACCESSION   DATCRO010000497 DATCRO010000000
VERSION     DATCRO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208053"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /locus_tag="VIN75_13535"
     CDS             <1..2545
                     /locus_tag="VIN75_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8975239.1"
                     /translation="LLADGVPATLGARAFDLLLALAGRPGQLLTKHALMDEVWPGVVV
                     EENNLAAQISALRKVLGGDLIATIPGRGYRFTAHLEAAGHEPSARDAVPQVADAPPAA
                     EAPALRTNLPARPAPLIGRGGDLAALAALVDAHRLVTLVGAGGMGKTRLAQAFLHAQQ
                     EAWRHGVCWIELATVADPGALPGAIASALGVRPPAGEPLAGLAGAVAPLQMLIAFDNA
                     EHLLDEVALVAQTLLDAAPGLRIIVTSQVPLKLAAERVFRLESLAVPQGPLPASQAIE
                     FGAVALFVDRAQAADARFALSDANAPAAIALCAALDGLALAIELAAARVPMFGVQQLA
                     ASMQDRLQVLTTSRDRAAPARHQTLRAALEWSHGLLNDREQTVFRRLSVFAGSTSLAL
                     IQRVVADPPHAGGLDEWTVLDALSGLVDRSLVAVVGGMDDTRPRYRMLASPRALALEQ
                     LRGAGEEDALRRRHALALAQLYDEHWHTRHSGALRVVDWKREVELDAPNASDAMAWAV
                     RTGMTDCALTIGATWLHAMNRFMHADRMGLADSCTALATLSRTPELRMRAALATARAW
                     TNPRKRRAFAAVDHALSLAREIDATAPDRWLLYRALAQWVVSGSSLADLSPDALKSAL
                     DELDRIEDPAWPPQRLDVGLEAHAFFLSRSDAGPEAATARLALIRRTAAIAAQLGDDV
                     ETSLNNLMDAELASGAVDAAARTGRALIEQLSGTRNEHILAYARLNLGACHLALDELD
                     AGRAMLQAAWAKAAAFEMQPYCADYLALLAALERRPRAAAWLAGYSDAGYRAKSEGRE
                     RNEAAASERMAALAIAQIGAEAFENGRAGGCALTDDEVARIAFAATDMA"
     gene            complement(2696..2971)
                     /locus_tag="VIN75_13540"
     CDS             complement(2696..2971)
                     /locus_tag="VIN75_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975240.1"
                     /translation="MTSSLSDLFEQQRIENARLSGQIVALEMAVKLLFMQHPNPNALA
                     GFYSKAIDGLLDITLATRMPEEMRDALDQARNGLLEALRQQPTLGGG"
     gene            complement(3032..>3958)
                     /locus_tag="VIN75_13545"
     CDS             complement(3032..>3958)
                     /locus_tag="VIN75_13545"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975241.1"
                     /translation="GEARVPVVVLLHGSEHDSALDFDSLQRRLPAEGVGAFVYDKRGT
                     GVSGGQYTQDYEQLARDAVAALAQARKLGGERVGRIGFEGPSQGGWVAPIAATHADVD
                     FVMVAFGLAVSVLEEDRSAVAKNMADHHHSAEDTAKAMALVDAADALLLDPTLATYDR
                     FAAVRDPLRKEPWYKDVHGDIAWALLPVQRDEVLALARKFDWHTPFPYDPLATIAAVK
                     PPQLWVLAEDDVDAPSAETARRLALLRMAGHPITSVVWPHTEHGIYEYETGPDGERTS
                     LRQPEGYLALLADFARGVPLARKYGVVTPSRP"
BASE COUNT          494 a         1493 c         1455 g          516 t
ORIGIN      
        1 cctgctggcc gacggcgtcc ccgccacgct gggggcccgc gcgttcgacc tgctgctggc
       61 gctcgccgga cgtcctggcc agctgctcac caagcacgcg ctgatggacg aggtctggcc
      121 cggcgtggtc gtcgaggaga acaacctcgc cgcgcagatc agcgcgctgc gcaaggtgct
      181 gggcggcgac ctgatcgcga ccatccccgg ccgcggctac cgcttcacgg cgcacctcga
      241 ggccgccggc cacgagccgt ccgcgcgcga cgccgtgccg caggtcgcgg acgcaccgcc
      301 cgccgctgaa gcgcccgcgc tgcgcaccaa cctgccagcg cgcccggcgc cgctgatcgg
      361 ccgcggcggc gacctggccg cgctggcggc gctggtcgac gcgcaccggc tggtcacgct
      421 ggtgggcgcc ggcggcatgg gcaagacgcg cctcgcgcag gcgttcctgc acgcgcagca
      481 ggaggcgtgg cggcatggcg tgtgctggat cgagctggcc acggtggccg acccgggcgc
      541 cctgcccggc gcgatcgcct cggccctggg cgtgcgcccg cccgccggcg agccgctggc
      601 cggcctggcc ggcgccgtgg cgccgctgca gatgctcatc gcgttcgaca acgccgaaca
      661 cctgctggac gaggtggcgc tcgtcgcgca gacgctgctg gacgccgcgc cgggcctgcg
      721 catcatcgtc accagccagg tgccgctgaa gctggcggcc gagcgcgtgt tccggctcga
      781 gtccctggcc gtgccccagg ggccgctgcc cgcgtcacag gcgatcgagt tcggcgccgt
      841 cgcgctgttc gtcgaccgcg cgcaggccgc ggacgcgcgc ttcgcgctca gcgacgccaa
      901 cgcgcccgcg gcgatcgcgc tgtgcgcggc gctcgacggc ctggcgctgg ccatcgagct
      961 ggccgccgcg cgcgtgccga tgttcggcgt ccagcagctg gccgcgtcga tgcaggatcg
     1021 cctgcaggtg ctcaccacga gccgcgaccg cgcggcgccg gcgcgccacc agacgctgcg
     1081 cgcggcgctc gagtggagcc acgggctgct caacgaccgc gagcagacgg tgttccgacg
     1141 gctgtcggtg ttcgcgggca gcacctcgct ggcactgatc cagcgcgtcg tggccgatcc
     1201 gccgcacgcc ggcggcctgg acgaatggac cgtgctggac gcgctgtcgg gcctggtcga
     1261 ccgctcgctc gtggccgtgg tcggcggcat ggacgacacc cggccgcgct atcggatgct
     1321 cgcctcgccg cgcgcgctgg cgctcgagca gctgcgcggc gcgggcgagg aggacgcgct
     1381 gcgccgacgc catgcgctgg cgctggcgca gctgtacgac gagcactggc acacgcgcca
     1441 ttccggcgcg cttcgtgtcg tcgactggaa gcgcgaggtg gagctcgacg cccccaacgc
     1501 cagcgacgcg atggcctggg ccgtgcgcac cggcatgacc gactgcgcgc tgaccatcgg
     1561 cgccacctgg ctgcacgcga tgaaccgctt catgcatgcc gaccgcatgg gcctggccga
     1621 cagctgcacc gcgctggcga cactctcgcg cacgcccgag ctgcgcatgc gcgccgcgct
     1681 cgcgaccgcg cgcgcatgga cgaacccgcg caagcggcgc gccttcgccg cggtcgacca
     1741 cgcgctgtcg ctggcgcgcg agatcgacgc caccgcgccc gatcgctggc tgctgtaccg
     1801 cgcgctcgcc cagtgggtgg tcagcggctc gagcctggcc gacctgtcgc ccgatgcgct
     1861 caagtccgcc ctcgacgagc tcgatcgcat cgaggatccc gcgtggccgc cgcagcgcct
     1921 ggacgtcggc ctcgaggcgc acgcgttctt cctcagccgc tccgatgcgg gtcccgaggc
     1981 cgccacggcg cggctcgcgt tgatccgccg cacggcggcc atcgcggcgc agctcggcga
     2041 cgacgtcgag acctcgctga acaacctgat ggacgccgag ctcgcatccg gcgccgtcga
     2101 tgccgccgcg cgcaccggcc gcgcgctgat cgagcagctg agcggcacgc gcaacgagca
     2161 catcctggcc tacgcgcgcc tgaacctggg cgcctgccac ctcgccctcg acgaactcga
     2221 cgcgggccgg gcgatgctgc aggcagcctg ggccaaggcc gccgcgttcg agatgcagcc
     2281 gtactgcgcc gactacctcg ccctgctggc cgcgctcgag cgccggccgc gcgccgccgc
     2341 gtggctggcc ggctattccg acgcgggcta ccgcgcgaaa tccgagggcc gcgagcgcaa
     2401 cgaggccgcg gccagcgagc gcatggcggc actggccatc gcccagatcg gcgccgaggc
     2461 gttcgagaac ggccgcgccg gcggctgcgc gctgaccgac gacgaggtcg cccgcatcgc
     2521 cttcgcggcg accgacatgg cctgagcgcg agccggcccg ggccgtgtgt cgcggacggt
     2581 cggcgatcgg ctccgcactc ggtgccgcac cgacctgtcg cctcgcgccc atcccctgtc
     2641 atcctgcgcg cagtcgcagg atccaccgcg gcgagccggg tcgctccggc agcgctcagc
     2701 cgccgcccag cgtcggctgc tgccgcagcg cctccagcag cccgttgcgc gcctggtcca
     2761 gcgcgtcgcg catctcttcg ggcatgcgcg tggccagcgt gatgtccagc agcccgtcaa
     2821 tcgccttgga gtagaagccc gccagcgcat tcgggttggg gtgctgcatg aacaggagct
     2881 tgaccgccat ctccagggcc acgatctgcc ccgagaggcg ggcgttctcg atgcgttgct
     2941 gctcgaagag gtcggacagg gagctggtca tggcaggcgc tttcgaggag gtggccggtg
     3001 caggatagcg cacggccggc gcgcccgcgg tctatggccg cgacggcgtg accacgccgt
     3061 atttccgtgc cagcggcacg ccgcgcgcga agtcggccag cagcgcgaga tagccctcgg
     3121 gctggcgcag cgacgtgcgc tcgccgtcgg ggccggtctc gtactcgtag atgccatgct
     3181 cggtgtgcgg ccacacgacc gaggtgatcg ggtggccggc catgcgcagc agcgcgaggc
     3241 ggcgggcggt ctcggcgctc ggcgcgtcga cgtcgtcctc ggccagcacc cacagctgcg
     3301 gcggcttgac ggcggcgatg gtggccagcg ggtcatacgg gaagggggta tgccagtcga
     3361 acttcctggc cagcgcgagc acctcgtcgc gctgcaccgg caggagggcc caggcgatgt
     3421 cgccgtgcac atccttgtac cagggctcct tgcgcagcgg atcgcgcacg gcggcgaagc
     3481 ggtcgtacgt ggccagcgtc gggtcgagca gcagcgcgtc ggcggcgtcg accagcgcca
     3541 tcgccttggc cgtgtcctcg gccgagtgat ggtggtcggc catgttcttg gcgacggccg
     3601 agcggtcctc ctcgagcacc gacacggcca ggccgaaggc caccatgacg aagtcgacgt
     3661 ccgcgtgcgt cgcggcgatc ggcgccaccc agccgccctg gctggggccc tcgaagccga
     3721 tgcggccgac gcgctcgccg ccgagcttgc gcgcctgcgc gagcgcggcg acggcgtcgc
     3781 gcgcgagctg ctcgtagtcc tgcgtgtact ggccgccgga tacgccggtg ccgcgcttgt
     3841 cgtagacgaa ggcgccgacg ccctcggcgg gcaggcggcg ctgcagcgaa tcgaagtcga
     3901 gcgcggagtc gtgctccgag ccgtgcagca gcacgacgac gggcacgcgc gcctcgcc
//
LOCUS       DATCRO010000498        15160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_939295, whole genome shotgun sequence.
ACCESSION   DATCRO010000498 DATCRO010000000
VERSION     DATCRO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939295"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIN75_13550"
     CDS             <1..495
                     /locus_tag="VIN75_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975242.1"
                     /translation="VQSSDPIAAFCDTLALAQQVTNGLASQGFEPVQLSIVGSGAALS
                     SPHTLDERVKEWGGRGSVWGALGGLAVGVVLIVPPVGSVIAMGPIVTLLISALEGAML
                     GGGFSAIVAALTGLGLPVEAAQHCEDAVMARRFLVFVHGPAVDVARARRLVAGMGLAG
                     VQVA"
     gene            545..1540
                     /locus_tag="VIN75_13555"
     CDS             545..1540
                     /locus_tag="VIN75_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8975243.1"
                     /translation="MRAMVLEQPGDSLVERERPVPPPRAGEVRLRVLACAVCRTDLHV
                     VDGDLPWRGPVVPGHEIVGVVQAVGQGVRSPSIGQRVGVPWLGGTCGHCDFCRRDREN
                     LCDTPEFTGYTRDGGFATHVLANAEYCIELPAPGSTALDDVHLAPLLCAGLIGWRSLQ
                     AAGDEVRDLGLYGFGAAAHLIAQVAVHRGMRVHAFTRRGDSAAQALARRLGAAWAGAS
                     DERPPIALDAAILFAPAGELVPLALSAVRKGGRVVCGGIHMSDIPSFPYERLWGERSI
                     TSVANLTRRDAREFMAVVAGMPLAVTTTSYPLARANEALADLREGRLVGAAVLVP"
     gene            1652..4234
                     /gene="mgtA"
                     /locus_tag="VIN75_13560"
     CDS             1652..4234
                     /gene="mgtA"
                     /locus_tag="VIN75_13560"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEY8975244.1"
                     /translation="MDQPACTESFWDRPLDRCTAGLACGVGGLPSTEAAARLERFGSN
                     ELVEAGRLPAWLDFLSHLRSPLVLLLLVACIGSALVGDRTSAVVIAVIVLGSVSLDFV
                     QERRAGDAARRLRESVGLTAAVLRDGRECRLPAADVVPGDVVLLAAGDLVPADGRLLE
                     ARDFFVRQSALTGESWPVEKHAGEAASHDGELGSATNAAFMGSSVLSGSARMLVCRTG
                     SRSFLGDVAATLRQVPPPTAFERGTRGFGEMVVKLTTMLVLFVVFVNLLAHRPVAETF
                     LFAIALAVGLTPELLPMIVSVTLSRGAMRMAAREVVVKRLAAIQNLGSMDVLCTDKTG
                     TLTEARVRLERHIDGDGHESARVLELAWINSHFESGLRSPLDEAILAAPGQGADRWRK
                     LDELPFGFERRRVSVLVEHDGRHLLVVKGAPEDVLAACTSIERASQATPLDAAARAAA
                     RERFDALARDGMRVLAVAWRELPADRLQACAGDEGELVFCGFLVFLDPPKAGVREALA
                     RLHALHVQVKVVTGDNEGVARHVCAQVGLPVKRLLTGAQVEALDDPALAVQARRTDVF
                     CRVSPAQKCRVLRALRGRGHVVGFLGDGVNDAPSLHEADVGISVDGAVDVAKEAADMI
                     LLRRDLAVLGDAVVEGRRTFANVMKYIMMATSSNFGNMLSMAAASLALPFLPMLPVQI
                     LLNNLLYDVSEIAIPFDEVDAAELVRPHDWDPGFIRRFTVLFGALSSVFDLATFWLLR
                     AGFGGGEAVFRTGWFIESLATQVLVIFVLRTRGLPWASRPHPWLTAGAIGVLGLAVVL
                     PFTPVGILLGFVAPPVSLLGAIAGLACAYLVSAEIAKRAFRKGAQGRGKGRHGRPVRS
                     RIPA"
     gene            4315..4698
                     /locus_tag="VIN75_13565"
     CDS             4315..4698
                     /locus_tag="VIN75_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975245.1"
                     /translation="MTASQAVVRIDHARAELLALGQPTAGAQRLHARTHDTGRHRSDV
                     RDAHEFFASVCDAIDAHDDALVVGARHAMADFERYLHKHRPDTAHRVAAFEVVDHQTD
                     AELVAFARSFFERRARLGGVFGQAG"
     gene            complement(4714..5097)
                     /locus_tag="VIN75_13570"
     CDS             complement(4714..5097)
                     /locus_tag="VIN75_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY8975246.1"
                     /translation="MEVETQQHLTNLRQLLAYRVRELESERHAAAIERAQSASEIDGA
                     TVLDRKDEAEAELREQEASRTEQHALSDLQRCRAALQRLDDGVYGDCADCEQPIPLAR
                     LMVQPEAERCAPCQSAWERRHPTAG"
     gene            5328..6077
                     /gene="phbB"
                     /locus_tag="VIN75_13575"
     CDS             5328..6077
                     /gene="phbB"
                     /locus_tag="VIN75_13575"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY8975247.1"
                     /translation="MSNDKRLALVTGGTGGLGQAICKRLADDGFAVVALHTPGNDRVG
                     AWLADREREGYAFAAEPVDVADFDACSRAVETIRGRHGPVSVLVNNAGITQDASFAKM
                     TREQWQRVLRVNLDSMFNMTKPVIEDMLAARWGRIVNISSVNGQRGAFGQANYVASKA
                     GIHGFTKSLAIEFARKGITVNTVSPGYLRTAMVEKVPVEILEGRILPQIPAGRLGEPD
                     EVAQLIGYLVSERGGFVTGANLAINGGQHMA"
     gene            6129..6581
                     /locus_tag="VIN75_13580"
     CDS             6129..6581
                     /locus_tag="VIN75_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975248.1"
                     /translation="MTRRQRSADSGAYPPAAQEFVTEADASQHHDAFASSCIEWVRQV
                     ERIEATSLAGLGALLQTVASRAASARTWAEWIELAGAGLQEAIDDAARTQGEWLSALM
                     QCQMETVKMLLDLGRPVLPGSRDGDVDLPIAWFTPWMAGIGMRPVQIA"
     gene            complement(6688..7212)
                     /locus_tag="VIN75_13585"
     CDS             complement(6688..7212)
                     /locus_tag="VIN75_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8975249.1"
                     /translation="MTSHPHSNPAAARAERARERQHHDQAHLARHHAEVLRSVRQLRQ
                     LAARVEQGGPDAHSRRLAAAIIGRMDELVPDVLTREARALSPAAGADDEPWARQAADQ
                     LRADHGWISTNWYELRPRLDGVAREQSWVDVDQLRDAADLFCTLLTEHVGLATTLLER
                     SGRRAEFEAGAAVH"
     gene            7425..8198
                     /locus_tag="VIN75_13590"
     CDS             7425..8198
                     /locus_tag="VIN75_13590"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY8975250.1"
                     /translation="MGTFNEATVLSVRHWTERLFSFTTTRDPGFRFTSGQFVMLGLMV
                     DGRPLMRAYSIASSAYDPELEFFSIKVPDGALTSRLQHIDPGDQVLLGNKPTGTLLLQ
                     NLLPGRALWLLATGTGLAPFLSLVRDPETYGQFERVVLVHGVRRVRDLAYADFLRHQL
                     PQSPWLGELVRARLLYCPLVTREPFERSGRIPDLMMRGALERDLGLPLLDPARDRCML
                     CGSAAMLSDTRAVLEGLGFVEGHASEPGSYVIERAFVER"
     gene            complement(8210..8890)
                     /locus_tag="VIN75_13595"
     CDS             complement(8210..8890)
                     /locus_tag="VIN75_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8975251.1"
                     /translation="MRALVNVALVAAGFALGAAAAPHCALMCGAPCAAVTRGRAREGT
                     LFHVGRLAGYAAAGAVAASALAVIAAWLSAKPWLAPLWTLVQLAFLALGAWWLATGRM
                     PARLLREGQSPAVDRRPCAPWRSAGAGLAWVAWPCGVLQGALLMATLGGDAAGGAAVM
                     AAFALASAPGLLAAPALWAVWRRFAGRGRDAAQVLGYRVAGAALCATASIALVHTLHD
                     RIAVLCRT"
     gene            complement(8907..10325)
                     /gene="hemN"
                     /locus_tag="VIN75_13600"
     CDS             complement(8907..10325)
                     /gene="hemN"
                     /locus_tag="VIN75_13600"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY8975252.1"
                     /translation="MHASVIPIAERPSAPPAALLRRIQGPGPRYTSYPTADRFHEHFG
                     ARELEAALRARRELSYRSVCTPLSLYVHIPFCESVCYYCACNKVITRHHERAGQYLDA
                     LDVEVALVNDQVGACRTVSQLHLGGGSPTFLTDAELDRLMATIRRSFFLAPDAEISIE
                     VDPRTVDAARLMHLRDAGFNRISFGVQDFDPGVQRAVHREQPFAQVQALMRSAREAGF
                     ASINVDLIYGLPRQTPETFRRTLQQVAGLRPDRIAAYGYAHLPVRFKPQRRIHADELP
                     DGPQRVAMLGDALAIFGAAGYDYIGMDHFALATDSLAVARREGRLHRNFQGYSTHPDC
                     DLIGLGVSAIGRVGDCYYQNDKTLDAYYAALEANRLPVARGLVLDFDDRVRRDIIMAL
                     MCHGRVDFDDVRRQHGVDMRKVCAGELVRLAPLAAEGLVELHEGGIRVTPSGWYVVRA
                     IAMVFDRHLDGGAARERFSRIV"
     gene            complement(10371..10604)
                     /locus_tag="VIN75_13605"
     CDS             complement(10371..10604)
                     /locus_tag="VIN75_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975253.1"
                     /translation="MRCRVLTILWPSFLMAIVLDGLVFSAVDPAEAGLDQWARSMSPL
                     GFYTLGFLLFWSIIACASGMTALLVVEQDGPPP"
     gene            complement(10630..10896)
                     /locus_tag="VIN75_13610"
     CDS             complement(10630..10896)
                     /locus_tag="VIN75_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975254.1"
                     /translation="MSRPVASAPPAPVAWWCFPTGWLALGLPLVAVVASTLSAAIAIR
                     GADPVIDGHRAPSRQAAEEQAEHGAQAEARLPAELARNHASMRK"
     gene            complement(10911..12368)
                     /gene="ccoG"
                     /locus_tag="VIN75_13615"
     CDS             complement(10911..12368)
                     /gene="ccoG"
                     /locus_tag="VIN75_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY8975255.1"
                     /translation="MSSEPRAVGVAPHRVIPIAADPATGLYRAEKKIHPREIHGRFAT
                     WRWILVAATQLAFYGLPWLEWNGRQALLFDLAARRFYIFGLVLYPQDFVYLAALLIVS
                     AYSLFLFTAVAGRLWCGFACPQTVYTEMFLWIERRFEGDRSARLRLDAAPWTVDKAWR
                     KSGKQAAWIALSLWTGFTFVGFFTPIRTLTQESVALSFGAWEWFWILFYGFATYGNAG
                     WMREQVCKYMCPYARFQSAMFDRDTMIVTYDGARGEPRGPRAKSLAHAELAAAGKGEC
                     IDCTMCVQVCPTGIDIRKGLQYECIGCAACIDACDAVMDKVNYPRGLIRFTTENGLER
                     GWTRTQMLRRVLRPRVLIYGGVLLLIVMAISTNLALRRPYRVDVLRDRATLAREVAGG
                     RIENVYRLQVMNAAESRQHFHVAVSGLDGIAIASDPVLDVDATGERSLAVRVQVPPET
                     AGRTGAGAHPIRFEIRRDGDERTPLVERSTFLIPR"
     gene            complement(12385..13290)
                     /gene="ccoP"
                     /locus_tag="VIN75_13620"
     CDS             complement(12385..13290)
                     /gene="ccoP"
                     /locus_tag="VIN75_13620"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY8975256.1"
                     /translation="MSDFFSAGWSVYIGVATIASLLGCLALLVITSRARVNPKDDTGH
                     VWDHDLRELNNPMPRWWMALFVITIVFAFGYLTLYPGLGSSKGLLGWSSASAYRDEQA
                     QADAQLATVYARYASTPAPELAQDKGAMAIGERLFMNNCSGCHGSDARGGKGFPNLTD
                     SDWLYGGSPETIVETITHGRQGTMPSMAAAVGTPDDVRNVAEYVLSLSGSPHDATAAA
                     AGQTRFIVCAGCHGIDGKGNQAVGAPNLTDAIWLHGTGREAIVRMIDNGKTNVMPAQE
                     HRLAPAQIHVVGAYVWSLSNGGSKN"
     gene            complement(13287..13436)
                     /locus_tag="VIN75_13625"
     CDS             complement(13287..13436)
                     /locus_tag="VIN75_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY8975257.1"
                     /translation="MDLDLVRYAVTVLSFVLFAGIVAWALSPRNRPAFDEAQQLPFLD
                     SGDAP"
     gene            complement(13448..14080)
                     /gene="ccoO"
                     /locus_tag="VIN75_13630"
     CDS             complement(13448..14080)
                     /gene="ccoO"
                     /locus_tag="VIN75_13630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY8975258.1"
                     /translation="MANDAATTRPGFSHARIETNNFLMIVLILVVVAIGGLVEIVPLF
                     FQRSTTQPVEGLKPYTALQLAGRDVYLREGCYGCHSQMIRPLRAETLRYGHYSMAGES
                     VYDHPFQWGSKRTGPDLARVGGKYSDDWHRLHLENPRDVVPESNMPAYPWLLTGKVDP
                     AGMAPRMRALRTVGVPYTDADITGAAAEVAGKTDEDALIAYLQVLGTAVK"
     gene            complement(14101..>15160)
                     /gene="ccoN"
                     /locus_tag="VIN75_13635"
     CDS             complement(14101..>15160)
                     /gene="ccoN"
                     /locus_tag="VIN75_13635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEY8975259.1"
                     /translation="ELEWPIALLLTVVWVIYAIVFFGTIGIRKVRHIYVANWFYGAFI
                     IAVALLHIVNSAEIPVGPLKSYSVYSGVQDAMVQWWYGHNAVGFFLTAGFLGMMYYFI
                     PKQAERPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWVQSVGMMFSLVLLAPSW
                     GGMINGIMTLSGAWHKLRDDPIMKFLIVSLSFYGMSTFEGPMMSIKTVNSLSHYTDWG
                     IAHVHSGALGWVGLITMGSLYYLIPRMFGLHQMYSVRAIELHFWISTIGIVLYIASMW
                     SAGVMEGVMWRSVNNDGTLAYSFVEVVKATFPFYVVRLGGGLLYLAGMLVMAWNTWKT
                     ATIGRYQPVRIPLAVANA"
BASE COUNT         2211 a         5161 c         5485 g         2303 t
ORIGIN      
        1 gtgcagtcct ccgatccgat cgccgcgttc tgcgacacct tggcgctcgc ccagcaggtg
       61 acgaacgggc ttgcaagcca ggggttcgag ccggtgcagc tctccatcgt gggcagcggc
      121 gcggcgctgt ccagcccgca tacgctggac gagcgcgtga aggagtgggg tggtcgcggc
      181 agtgtctggg gcgcgctcgg cggcctggcc gtgggagtcg tgctgatcgt gccgcccgtc
      241 gggtccgtca tcgccatggg gccgatcgtc acgctgttga tctcggcgtt ggaaggcgcc
      301 atgctcgggg gcggcttctc cgcgatcgtc gcggccctga ccggactggg gctgccggtc
      361 gaggcggcgc agcactgcga ggacgccgtg atggcgcgac gcttcctcgt ctttgtccat
      421 gggccggcgg tcgacgtggc gcgcgcacgc aggctggtcg ccggaatggg acttgccggg
      481 gtgcaggtgg cctgagaccc cgctgatggc ggttgtcgag ccccttgccg ggccggagat
      541 cgacatgcga gcaatggttc tggaacagcc gggcgattcg ctggtggagc gcgagcgtcc
      601 tgtgccgccg ccccgggccg gggaggtccg cttgcgcgtc ctggcctgcg ccgtctgccg
      661 taccgacctg cacgtcgtcg acggcgacct gccgtggcgc ggaccggtgg tgcccggtca
      721 cgagatcgtc ggcgtcgtcc aagccgtcgg acagggcgtg cgctcgccgt cgatcgggca
      781 gcgcgtcggc gtgccctggc tcggcggtac ttgcgggcat tgcgacttct gccgacgcga
      841 ccgggagaac ctgtgcgaca cgccggagtt cacgggctac acgcgcgacg gcggcttcgc
      901 cacgcatgtc ctggccaatg cggaatactg catcgagctg ccggcgcccg gctcgaccgc
      961 gctcgacgac gtccacctgg cgccgctcct gtgcgccggc ctgatcggct ggcgttcgct
     1021 ccaggccgcg ggcgacgagg tgcgcgacct gggcctctac ggtttcggcg ccgcggcgca
     1081 cctgatcgcc caggtggcgg tccatcgcgg catgcgcgtc catgccttca cccgccgcgg
     1141 cgacagcgcc gcccaggcgc tggctcgacg gctgggtgcc gcctgggccg gtgcgtccga
     1201 cgagcgcccg cccatcgcgc tcgatgccgc gatcctgttc gcgccggccg gcgaactggt
     1261 gcccctggcg ctgtcggcgg tgcgcaaggg cggacgcgtc gtgtgcggcg gcatccacat
     1321 gagcgacatc ccgtcgttcc cgtacgagcg gctgtggggc gagcgcagca tcacgtcggt
     1381 cgcgaacctg acccgacgcg atgcacgcga attcatggcg gtggtcgcgg gcatgccgct
     1441 cgcggtgacg acgacgagct acccgctcgc acgtgccaat gaggccctgg ccgacctgcg
     1501 cgagggccgg ctcgtcggcg cggccgtgct cgttccctga agtgagatcg gcaaggcgcc
     1561 ggcttgcgac gcctcaaggc caggccggct gcggcacgcc atgctcgccg tcctgggacg
     1621 tggcgtcgcg tctcgccggc acggagatga catggaccag cccgcttgca ccgagtcgtt
     1681 ctgggatcga ccgctcgacc ggtgcaccgc cggactcgcg tgcggcgtcg gcggactgcc
     1741 atcgaccgag gcggctgctc gcctcgagcg cttcggatcg aacgaactcg tggaagcggg
     1801 acgcctgccc gcctggctgg acttcctgtc ccacctgcgc agcccgctcg tcctgctgct
     1861 gctggtcgcc tgcatcggct cggcgctggt cggggaccgc accagcgcgg tcgtcatcgc
     1921 cgtcatcgtg ctgggcagcg tctcgctcga cttcgtgcag gagcgccggg cgggcgacgc
     1981 ggcgcgccgg ctgcgggagt cggtcgggct gacggctgcc gtgctgcgcg acgggcgcga
     2041 atgccgcctt cccgctgcgg acgtcgttcc cggggacgtc gtccttctgg ctgcgggcga
     2101 tctcgtgccg gccgatggac gcttgctcga ggcgcgtgac ttcttcgtgc ggcaatcggc
     2161 gctcaccggg gaatcgtggc cggtcgagaa gcacgccggc gaggccgcga gtcacgacgg
     2221 cgagctcggt tcggcgacga acgccgcgtt catgggatcc tcggtactca gcggcagcgc
     2281 ccgcatgctg gtctgccgca cgggcagccg gtcgttcctg ggtgacgtgg cggccacgct
     2341 gcgccaggtg ccgccgccca cggccttcga gcgcggcacc cggggcttcg gcgagatggt
     2401 ggtcaagctc acgaccatgc tcgtgctgtt cgtcgtgttc gtgaacctgc tggcgcaccg
     2461 gccggtcgcc gagacctttc tcttcgcgat cgcgcttgcc gtcggcctca cgcccgagct
     2521 gctgccgatg atcgtctcgg tgacgctgtc gcgtggcgcc atgcggatgg ctgcgcgcga
     2581 ggtcgtcgtc aagcggctcg cggcgatcca gaacctgggc agcatggacg tgctgtgcac
     2641 ggacaagacg gggacgctga ccgaggcccg cgtccgcctg gaaaggcaca tcgacggcga
     2701 cggccacgaa agcgcgcgcg tgctggagct ggcatggatc aacagccact tcgagtcggg
     2761 gcttcgaagc ccgctcgacg aggccatcct cgcggcgccc gggcagggcg ccgaccgctg
     2821 gcgcaagctc gacgagctgc cgttcggctt cgagcggcgc cgcgtctccg tgctggtgga
     2881 gcatgacggc cggcacctgc tggtagtcaa gggtgcgccg gaggacgtgc tcgcggcgtg
     2941 cacgtcgatc gaacgcgcga gccaggccac accgctcgac gccgccgcgc gcgccgccgc
     3001 cagggaacgg ttcgacgcgc tcgcgcggga cggcatgcgg gtgctggcgg tcgcatggcg
     3061 cgagctgcct gcggaccggc tgcaggcctg cgcgggcgac gagggcgagc tcgtcttctg
     3121 tggcttcctc gtcttcctgg acccgcccaa ggcgggcgtg cgcgaggcgc tggcacggct
     3181 gcatgcgctg cacgtgcagg tcaaggtggt gacgggcgac aacgaggggg tggcacgcca
     3241 cgtgtgcgcc caggtcgggc tgcccgtgaa gcgcctgctg accggcgcac aggtcgaagc
     3301 gctcgacgac cccgcgctgg ccgtgcaggc caggcgcacg gacgtcttct gccgtgtgtc
     3361 gccggcgcag aaatgccgcg tcctgcgcgc gctgcgcggc cggggacacg tggtcggctt
     3421 cctcggcgac ggcgtcaacg atgcgccgtc gctgcacgag gccgacgtcg gcatctcggt
     3481 cgacggcgcc gtcgacgtcg ccaaggaggc ggccgacatg atccttctgc ggcgggacct
     3541 ggccgtgctg ggggacgcgg tggtcgaggg gcgccgcacc ttcgccaacg tcatgaagta
     3601 catcatgatg gccacgagct cgaacttcgg gaacatgctg agcatggcgg ccgcctcgct
     3661 ggcgctgccg ttcctgccga tgctgcccgt gcagatcctg ctgaacaacc tgctgtacga
     3721 cgtctccgag atcgcgatcc cgttcgacga ggtggacgcc gcggagctgg tgcgtccgca
     3781 tgactgggat cccgggttca tccgtcgctt caccgtgctg ttcggcgcgc tcagctcggt
     3841 ctttgacctc gccacgttct ggctgctgcg cgcaggcttc ggcggcggcg aggccgtttt
     3901 ccggacgggt tggttcatcg aatcgctcgc gacgcaggta ctggtgatct tcgtcctgcg
     3961 cacgcgcggc ctgccgtggg ccagccgccc ccacccctgg ctgacggccg gcgccatcgg
     4021 cgtcctcggg ctggccgtcg tgttgccttt cacgccggtg gggatcctcc tgggattcgt
     4081 cgcaccgccg gtgtccctgc tgggcgcgat cgccgggctc gcatgcgctt atctcgtctc
     4141 ggcggaaatc gcgaagcgcg ccttcaggaa gggcgcgcaa ggccgcggca aaggccggca
     4201 tgggcgtccg gtgcggtcgc gcattccggc gtgagccttg cacttgacca gacgcaaggc
     4261 cggccgtgcc tggtgcgccg acgatgtctt cttcttcacc agagggcaag acacatgacc
     4321 gcatcccagg ccgtcgtccg catcgaccac gcgcgggccg agctccttgc gctcgggcag
     4381 ccgaccgccg gcgcgcagcg gctgcatgcg cggacgcacg acacgggccg ccaccgcagc
     4441 gacgtgcgcg acgcgcacga attcttcgcc tccgtctgcg acgcgatcga cgcccacgac
     4501 gacgcgctgg tcgtcggcgc acggcatgcg atggccgact tcgagcgcta cctgcacaag
     4561 catcgcccgg acaccgcgca tcgcgtcgcg gccttcgagg tcgtcgatca ccagaccgac
     4621 gccgaactcg tggcgttcgc ccgcagcttc ttcgagcgcc gggcgcggct gggcggtgtg
     4681 ttcggccagg ctggctgacg ccggcgcggc agctcagccc gccgtcgggt ggcggcgctc
     4741 ccaggcggac tggcagggcg cgcagcgctc ggcctcgggc tgcaccatca ggcgtgcgag
     4801 cggaatgggt tgctcgcagt cggcgcaatc gccgtagacg ccgtcgtcca ggcgttgcag
     4861 cgcggcccga cagcgctgca ggtccgagag ggcatgctgt tccgtgcgcg acgcttcctg
     4921 ttcccgcagc tcggcctcgg cctcgtcctt gcgatcgagc accgtcgcgc cgtcgatctc
     4981 cgatgcgctc tgcgcgcgct cgatggcggc cgcatgacgc tcggattcga gttcgcgcac
     5041 ccgataggcg agcagctggc gcaggttggt caggtgttgc tgggtctcga cttccatggt
     5101 ggctgctcat gatggggcgg ttgcgatgcg tccagcatgc gattgccgcg gcgccggcgc
     5161 gttgagcttc gtcaatcggc cgctgtcgta gcgagcgggg cacggaaatc ctcgggcggc
     5221 gccgcggacg acattgcgcc cgcgcaaggc gatgcccaac ggttcgccta gcctgtgggg
     5281 acggttgcca ccgcttcggc agccgtaccc atcgaaggag tccaccgatg agcaacgaca
     5341 agcgcctcgc cctcgtcacg ggcggcacgg gcggcctcgg ccaggccatc tgcaagcgcc
     5401 tcgccgacga cggcttcgcc gtggtggcgc tgcacacgcc gggcaacgac cgtgtcggtg
     5461 cctggctggc cgatcgcgag cgcgagggct acgccttcgc cgccgagccg gtggatgtcg
     5521 ccgacttcga cgcctgcagc cgggcggtcg agacgatccg cgggcgtcac ggcccggtct
     5581 cggtgctggt caacaacgcg ggcatcacgc aggacgcgag cttcgccaag atgacgcgcg
     5641 agcagtggca gcgcgtgctg cgggtgaacc tcgactcgat gttcaacatg accaagcccg
     5701 tcatcgagga catgctcgcg gccaggtggg gacgcatcgt caacatctcg tccgtcaacg
     5761 gccagcgcgg ggcgttcggc caggccaact atgtcgcctc caaggcgggc atccacggct
     5821 tcacgaagtc gctggccatc gagttcgcgc gcaagggcat caccgtgaac acggtctcgc
     5881 ccggctacct gcgcacggcc atggtggaga aggtgcccgt cgagatcctg gaaggccgga
     5941 tcctgccgca gatcccggcc gggcggcttg gcgagcccga tgaagtggcg cagttgatcg
     6001 gctacctcgt gtcggagcga ggcggcttcg tcacgggcgc caacctggcg atcaacggcg
     6061 gccagcacat ggcctgatcg cagatcaagt ggagcacggc gacagcgcct tcattccgga
     6121 gacccatgat gaccagacga cagcgttccg ccgacagcgg cgcctacccc ccggcggccc
     6181 aggagttcgt gacagaggcc gacgcgagcc agcatcacga tgccttcgcc tcgagctgca
     6241 tcgagtgggt ccggcaggtc gaacgcatcg aggccaccag cctggccggc ctcggagccc
     6301 tgctgcagac cgtcgccagc cgggccgcct cggcgcgcac ctgggcggag tggatcgagc
     6361 tggcgggcgc cggcctgcag gaggcgatcg acgatgcggc ccgcacgcag ggcgaatggc
     6421 tgtcggcgct gatgcagtgc cagatggaga ccgtcaagat gctgctcgat ctcgggcggc
     6481 ccgtgctgcc gggctcacgc gatggcgacg tcgatctgcc gatcgcctgg ttcacgccat
     6541 ggatggccgg gatcggcatg cggcccgtgc agatcgcatg acttgcggcc ggagggcaag
     6601 gaaccatgcc tcctgccttc gaggcctgac gtcgcttgcc gagcatcgcg cagcatcgtc
     6661 caggcgatgg ctgcctgtcc gcttcggtca gtgcacggcg gcgccggcct cgaactcggc
     6721 gcgccgcccg ctgcgttcca gcagcgtcgt cgccaggccg acgtgttccg tcagcagggt
     6781 gcagaacagg tcggcggcgt ctcgcagctg atcgacgtcc acccaggact gctcgcgcgc
     6841 cacgccgtcc agccggggac gcagctcgta ccagttcgtc gagatccacc cgtggtcggc
     6901 gcgcagctgg tcggcggcct gccgcgccca cggctcgtcg tcggcgccgg cggcgggcga
     6961 cagcgctcgc gcttcccggg tcaggacgtc tggcacgagc tcgtccatgc ggccgatgat
     7021 cgcggcggcg aggcggcggg aatgagcgtc cggcccgcct tgctcgacgc gggccgccaa
     7081 ctggcgcagc tggcgcacgg accggagaac ttcggcgtgg tgacgagcca gatgggcctg
     7141 gtcgtggtgc tggcgctcgc gcgcgcgctc ggcgcgggcg gcggccgggt tggaatgggg
     7201 gtgcgaggtc atgaacgggc ctgtcgatga acgaggtggt gctgcggatg cgccgcagcg
     7261 cagccgggca cggtggttga agtctcattt caaggcatcg agtccggcca cggcttgtcc
     7321 tgcgtcaacg acattcggcg acaggctcga tggcgccgct tgttccggcg caaggacagg
     7381 ccggccccga ccgccgatgc ttggcgcaac ctggagccat atcgatggga accttcaacg
     7441 aggcgacggt gctgtccgtg cgccactgga ccgaacgcct gttctccttc accacgacgc
     7501 gcgaccccgg attccgcttc accagcggtc aattcgtgat gctcggcttg atggtcgacg
     7561 gccgcccctt gatgcgggcc tacagcatcg cgagttcggc gtacgacccg gaactcgagt
     7621 tcttcagcat caaggtgccc gacggtgcct tgacgtcgcg cctgcagcac atcgatccgg
     7681 gcgaccaggt gctgctcggc aacaagccga cggggacctt gctgctgcag aacctcctgc
     7741 ccggccgcgc gctgtggctg ctggccaccg gtaccggact cgcccccttc ctgagcctgg
     7801 tgcgagaccc cgagacctac ggccagttcg agcgcgtggt gctcgtgcac ggcgtccgga
     7861 gggtgcgcga cctggcgtat gcggatttcc tgcgacacca gcttccgcag agcccgtggc
     7921 tcggcgagct cgtccgggcc aggctgcttt attgcccgct cgtcacccgc gagccgttcg
     7981 agcgaagcgg ccgcatcccg gacctgatga tgcgcggcgc gttggagagg gatctcggcc
     8041 ttccgctgct cgacccggcg cgcgatcgtt gcatgctgtg cggcagcgcg gcgatgctgt
     8101 cggacacgcg cgccgtgctc gaagggctcg gcttcgtcga aggccacgcg tccgagccgg
     8161 gcagctacgt catcgagcgg gcgttcgtcg agcgttgaac ggcctcgcct caggtgcggc
     8221 acaggacggc gatccggtca tggagcgtat ggaccagggc gatcgacgcc gtggcgcaga
     8281 gcgcggcgcc ggcgacccgg tagcccagca cctgcgcggc atcgcggccg cgcccggcga
     8341 accggcgcca gacggcccag agcgcaggcg cggccagcag tcccggcgcg gaggcgagcg
     8401 cgaaagccgc catcacggcg gcaccgcccg cggcgtcgcc gccgagcgtc gccatcagca
     8461 gggcgccttg caggacgccg cagggccacg ccacccaggc gagcccggcc cccgccgacc
     8521 gccagggtgc gcagggccgt cgatccacgg cgggtgactg gccctcgcgc aacaaccggg
     8581 cgggcatccg gcccgtcgcc agccaccacg cgcccagggc caggaatgca agctggacga
     8641 gcgtccacag cggcgccagc cacggcttgg ccgacagcca tgcggcgatg acggccaggg
     8701 cgctcgcggc gacggcgccg gccgccgcat agccggcgag gcggcccaca tggaacagcg
     8761 tgccttcgcg cgcgcggccg cgcgtgacgg ccgcgcacgg cgcgccgcac atgagggcgc
     8821 agtgcggggc ggccgcggcg ccgagcgcga atccggcggc gaccagcgcg acgttcacga
     8881 gggcacgcat tgggtgtgaa caggccctag acgatgcgcg agaagcgctc gcgcgccgcg
     8941 cccccgtcga ggtgccggtc gaacaccatg gcgatcgccc gcacgacgta ccagccgctc
     9001 ggcgtgacgc ggatcccgcc ttcgtgcagt tcgacgaggc cctcggcggc caggggggcg
     9061 agccgcacca gttcgccggc gcacaccttg cgcatgtcga cgccatgttg cctgcgcacg
     9121 tcgtcgaagt cgactcgtcc atggcacatg agcgccatga tgatgtcgcg ccggacgcgg
     9181 tcgtcgaaat cgagcaccag gccgcgcgcc accggcagcc ggttggcctc gagcgccgca
     9241 tagtaggcgt ccagcgtctt gtcgttctgg tagtagcagt cgcccacgcg gccgatcgcc
     9301 gagacgccca ggccgatcag gtcgcagtcc gggtgcgtgc tgtagccctg gaaattgcgg
     9361 tggaggcggc cttcgcgcct ggccacggcc aggctgtcgg tcgccagggc gaagtggtcc
     9421 atgccgatgt agtcgtaccc cgcggcgccg aagatcgcca aggcgtcgcc gagcatggcg
     9481 acgcgctgcg gcccgtcggg cagctcgtcg gcatggatgc ggcgttgcgg cttgaagcgc
     9541 acgggcaggt gtgcgtagcc gtatgccgcg atccggtcgg ggcgcaggcc ggccacctgc
     9601 tgcagcgtgc ggcggaaggt ctcgggcgtc tggcgtggca ggccgtagat caggtccacg
     9661 ttgatcgacg cgaatccggc ctcgcgtgcc gagcgcatca gcgcctgcac ctgcgcgaag
     9721 ggctgttctc gatggacggc gcgctgcacg ccgggatcga agtcctgcac gccgaagctg
     9781 atccggttga agccggcgtc gcgcaggtgc atcaggcgcg ccgcgtccac cgtgcgcgga
     9841 tcaacctcga tcgagatctc cgcatcaggc gcgaggaaga aggatcggcg gatcgtcgcc
     9901 atcagccggt cgagttcggc gtccgtgagg aatgtcggcg agccgccgcc cagatgcagc
     9961 tgcgacacgg tgcggcacgc gcccacctgg tcgttgacca gcgccacttc cacgtcgagg
    10021 gcgtcgaggt actgcccggc ccgctcatgg tggcgggtga tcaccttgtt gcacgcgcag
    10081 tagtagcaga cggactcgca gaacgggatg tgcacgtaga gcgacagcgg cgtgcagacg
    10141 ctcctgtagg acagctcgcg ccgcgcgcgc agggccgcct cgagttccct ggcgccgaag
    10201 tgctcgtgga atcgatccgc ggtgggatag gaggtgtagc gggggccggg gccctggatg
    10261 cgtcgcagga gggccgcagg cggcgcggac gggcgctcgg cgatcgggat gacggatgcg
    10321 tgcatgcccc cgatggtgcg gcgtgcgacg accggctcgc ttgcgccgcg tcaaggcggc
    10381 gggccgtcct gttcgaccac gagcagggcg gtcatgccgc tggcgcacgc gatgatgctc
    10441 cagaacagca ggaatcccag cgtgtagaag ccgagcggcg acatgctccg ggcccactgg
    10501 tcgaggccgg cttcggccgg atcgacggcg gagaaaacga ggccgtccag gacgatggcc
    10561 atcaggaacg acggccagag gatggtgagg acgcgacacc gcatgacctg ctcccgtcgt
    10621 tgctccgcat cactttcgca tcgaggcatg gttgcgcgcc agctcggccg gcaaccgcgc
    10681 ctcggcttgc gcgccatgct cggcctgctc ctcggctgcc tggcgcgagg gggcgcgatg
    10741 accgtcgatg accgggtcgg cgccccggat ggcgatcgcg gccgacagcg tcgatgccac
    10801 gaccgcgacc agcggcaggc cgagcgccag ccatccggtc gggaagcacc accaggcgac
    10861 gggcgccgga ggcgcagagg caacgggtcg ggacatggcg gtctcctcgt tcagcggggg
    10921 atcaggaagg tcgagcgctc gaccaggggc gtgcgctcgt cgccgtcgcg gcggatctcg
    10981 aagcggatcg gatgggcgcc ggctccggtg cgcccggcgg tctccggcgg gacctgcacg
    11041 cggaccgcga gcgatcgttc gccggtcgcg tccacatcca gcacggggtc cgaggcgatg
    11101 gcgatgccgt ccagccccga gacggcgaca tggaagtgct gccgcgactc ggcggcgttc
    11161 atgacctgca ggcggtagac gttctcgatg cggccgccgg cgacctcgcg cgccagcgtt
    11221 gcgcggtcgc gcagcacgtc gacacgataa ggacgccgaa gcgcgagatt cgtggagatg
    11281 gccatcacga tgagcagcag tacgccgcca tagatcagca cccggggccg cagcacgcgg
    11341 cgcagcatct gcgtcctcgt ccatccgcgc tcgaggccgt tctccgtcgt gaagcggatc
    11401 aacccgcgcg ggtaattcac cttgtccatc acggcgtcgc aggcgtcgat gcaggcggcg
    11461 cagccgatgc attcgtattg caggcccttg cggatgtcga tgccggtggg gcagacctgg
    11521 acgcacatcg tgcagtcgat gcactcgccc ttgccggcgg cagccagctc ggcatgcgcc
    11581 agcgatttcg cacgcgggcc gcgcggctcg ccgcgggcgc cgtcataggt cacgatcatc
    11641 gtgtcgcggt cgaacatggc gctctggaag cgcgcgtacg ggcacatgta cttgcacacc
    11701 tgctcgcgca tccagccggc attgccgtag gtggcgaagc cgtagaacag gatccagaac
    11761 cactcccacg cgccgaacga cagggcgacg ctctcctgcg tcagcgtgcg gatcggcgtg
    11821 aagaagccga cgaaggtgaa gccggtccac agggagagcg cgatccaggc ggcctgcttg
    11881 cccgacttgc gccacgcctt gtcgacggtc cagggcgccg cgtccaggcg cagccgcgcg
    11941 ctgcggtcgc cctcgaagcg gcgctcgatc cacaggaaca tctcggtgta gaccgtctgc
    12001 gggcaggcga agccgcacca aagtcgtccc gcgaccgccg tgaacaggaa cagcgaatag
    12061 gcgctgacga tcagcagcgc cgcgaggtag acgaagtcct gcggatacag gacgaggccg
    12121 aagatgtaga aacggcgggc ggcgagatcg aacaggagcg cctggcgccc gttccattcg
    12181 agccagggca ggccgtagaa ggccagctgc gtggcggcga cgaggatcca gcgccaggtg
    12241 gcgaagcggc cgtggatctc gcgcgggtgg atcttcttct cggcgcggta cagcccggtc
    12301 gccgggtcgg cggcgatggg gatcacccgg tgcggcgcga cgccgaccgc ccgaggttcg
    12361 ctgctcatgc gctcgtcccc atgctcagtt cttcgagccg ccgttcgaca ggctccacac
    12421 gtaggccccg accacgtgga tctgcgccgg ggcgaggcga tgctcctgcg cgggcatgac
    12481 gttggtcttg ccgttgtcga tcatgcgcac gatcgcttcc cggccggtgc cgtgcagcca
    12541 gatggcgtcg gtgaggttgg gcgcaccgac ggcctggttg cccttgccat cgatcccatg
    12601 gcagccggcg cagacgatga acctggtctg gccggccgcc gcggccgtcg catcgtgcgg
    12661 gctgcccgac aggctcagca cgtactccgc cacgttgcgc acgtcgtcgg gcgtgcccac
    12721 cgcggcggcc atcgacggca tcgtgccctg gcggccgtgc gtgatggtct cgacgatggt
    12781 ctccgggctg ccgccgtaga gccagtcgct gtcggtcagg ttcgggaagc ccttgccgcc
    12841 gcgcgcgtcc gagccgtggc agccggagca gttgttcatg aacaggcgct ccccgatggc
    12901 catggcgccc ttgtcttgcg caagctcggg cgcgggggtc gatgcgtatc tggcgtacac
    12961 cgtggcgagc tgcgcgtcgg cctgggcctg ctcgtcgcgg taggccgacg cgctgctcca
    13021 gccgagcagt cctttcgagc tgcccaggcc gggatacagg gtgaggtacc cgaacgcgaa
    13081 cacgatcgtg atgacgaaca gcgccatcca ccagcgcggc atcgggttgt tcagctcgcg
    13141 caggtcatgg tcccacacgt ggccggtgtc gtccttcggg ttgacgcggg cgcggctcgt
    13201 gatcacgagc agcgcgaggc agccgagcag gctggcgatg gtggccacgc cgatgtagac
    13261 cgaccagccg gccgagaaga agtcgctcat ggggcgtctc cggagtcgag gaagggcagt
    13321 tgctgggcct cgtcgaaggc cgggcggttg cgcggcgaca gcgcccacgc gacgatgccc
    13381 gcgaacagca cgaaggacag gacggtgacg gcgtagcgca cgagatcgag atccatggcc
    13441 agggtcctca cttgacggcg gtgccgagca cctgcaggta ggcgatgagc gcgtcctcgt
    13501 cggtcttgcc cgcgacctct gccgcggcgc cagtgatgtc ggcgtcggtg tacggcacgc
    13561 cgaccgtgcg cagcgcgcgc atgcgcggcg ccatcccggc cggatcgacc ttgcccgtca
    13621 gcagccaggg gtaggccggc atgttggact cgggcaccac gtcgcgcggg ttctccaggt
    13681 gcaggcgatg ccagtcgtcg ctgtacttgc cgccgacccg ggccaggtcg ggcccggtgc
    13741 gcttgctgcc ccactggaac gggtggtcat agacggactc cccggccatc gagtaatggc
    13801 cgtagcgcag cgtctcggcg cgcagcgggc ggatcatctg cgagtggcag ccgtagcagc
    13861 cttcgcgcag gtagacgtcg cgcccggcca gctggagcgc cgtgtagggc ttgagtccct
    13921 cgaccggctg cgtggtggag cgctggaaga acagcgggac gatctcgacc agtcccccga
    13981 tcgcgacgac cacgaggatc agcacgatca tgaggaagtt gttggtctcg atcctggcgt
    14041 gcgagaaccc gggccgggtc gtcgcggcat cgttggccat gtcggtttcc ttttcagagg
    14101 tcaggcgttc gccacggcca gcgggatgcg cacgggctgg tagcggccga tcgtcgcggt
    14161 cttccacgtg ttccaggcca tgacgagcat gccggccagg tacagcaggc ccccacccag
    14221 gcgcacgacg tagaagggga acgtggcctt gaccacctcg acgaagctgt acgcgagcgt
    14281 gccgtcgttg ttgacgctgc gccacatcac gccttccatg acgccggcgc tccacatcga
    14341 ggcgatgtac agcacgatgc cgatggtgga gatccagaag tgcagctcga tggcgcgcac
    14401 gctgtacatc tggtgcaggc cgaacatgcg cgggatcagg tagtacagcg accccatcgt
    14461 gatcaggccc acccagccga gggcgcccga atggacgtgg gcgatgcccc agtccgtgta
    14521 gtgcgacagc gagttgaccg ttttgatcga catcatcggg ccctcgaagg tcgacatgcc
    14581 gtagaacgac aggctcacga tcaggaactt catgatcggg tcgtcgcgca gcttgtgcca
    14641 ggcgcccgac aaggtcatga tcccgttgat catgccgccc cagctgggcg ccagcagcac
    14701 cagcgagaac atcatgccca cgctctgcac ccagtccggc agcgccgtgt agtgcaggtg
    14761 gtgcgggccc gcccacatgt aggtgaagat cagcgcccag aagtgcacga tcgacagccg
    14821 atagctgtag accgggcgct cggcctgctt ggggatgaag tagtacatca tcccgaggaa
    14881 cccggccgtc aggaagaagc ccaccgcgtt gtggccgtac caccactgca ccatcgcgtc
    14941 ctgcacgccc gagtacaccg agtagctctt cagcggcccg accgggatct cggcgctgtt
    15001 gacgatgtgc agcagcgcga ccgcgatgat gaaggcgccg tagaaccagt tggccacgta
    15061 gatgtggcgc accttgcgga tgccgatggt gccgaagaag acgatcgcgt agatcaccca
    15121 cacgaccgtc agcagcaggg cgatcggcca ctcgagttcg
//
LOCUS       DATCRO010000499         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_534406, whole genome shotgun sequence.
ACCESSION   DATCRO010000499 DATCRO010000000
VERSION     DATCRO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534406"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VIN75_13640"
     CDS             complement(<1..1233)
                     /locus_tag="VIN75_13640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY8975260.1"
                     /translation="MGIHVIKMPDIGEGIAEVELVAWNVKVGDNVVEDMNLADVMTDK
                     ATVEIPSPVHGKVLALGGKVGDVMAVGSELIRIEVEGAGNVKDGAAAPAPAAAAPAPA
                     PKAAPAPAPAVATTPAPAPAPVAKAPTPAPAKAPAAGAQKITRAPTDKPIASPAVRAH
                     AWDLGIDLQYVMGTGTAGRITHEDIEAYAARGLVGGTGASGSVDTRYAQRTEEEQIPV
                     IGLRRKIAQKMQDAKRRIPHFTYVEEIDVTELEALRGKLNQKHGATRGKLTMLPFIMR
                     AVVLAVRDFPQMNARFDDEAGVVTRHAAVHLGMATQTDAGLMVPVLQHAETLDLWATA
                     AEVSRLAAGARTGKSAREELTGSTITITSLGALGGIVTTPVINSPEVAIVGVNRVVER
                     PMIRDGLVVARKMMNLSSS"
     gene            complement(1238..2293)
                     /locus_tag="VIN75_13645"
     CDS             complement(1238..2293)
                     /locus_tag="VIN75_13645"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8975261.1"
                     /translation="MQDKKTLAAQPQGPTAPMTMIQALRSAMDVMLARDPNVVVFGED
                     VGYFGGVFRCTEGLQAKYGAQRVFDAPISEGGIVGAAVGMGAYGLRPVVEIQFADYFY
                     PASDQIVSEAARLRYRSAGDFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTHVCGLRTV
                     MPSNPYDAKGLLISCIENDDPVIFLEPKRLYNGPFDGHHDKPVVPWSKHKLGEVPEGY
                     YTVPLDSASVYREGSQLTVLTYGTMVFVSEAAAAETGIDAEIIDLRSIWPLDLDTIVR
                     SVKKTGRCVVVHEATKTSGFGAELAALVQEHCFYHLEAPIERVAGWDTPYPHAQEWAY
                     FPGPDRVGAAFKRAMEK"
BASE COUNT          308 a          791 c          829 g          385 t
ORIGIN      
        1 cgacgacgac aggttcatca tcttgcgcgc gacgaccagg ccgtcgcgga tcatcgggcg
       61 ctcgacgacg cggttgacgc cgacgatggc cacctccggc gagttgatca ccggcgtcgt
      121 gacgatgccg cccagcgcgc cgaggctggt gatcgtgatc gtcgagccgg tgagttcctc
      181 gcgcgcgctc ttgcccgtgc gcgcgcccgc ggccaggcgc gagacctcgg cggcggtggc
      241 ccacaggtcc agtgtctcgg catgctgcag caccggcacc atcaggccgg catcggtctg
      301 cgtggccatg cccaggtgca ccgcggcgtg gcgcgtgacc acgccggcct cgtcgtcgaa
      361 gcgcgcgttc atctgcggga agtcgcgcac ggccagcacc acggcgcgca tgatgaacgg
      421 cagcatcgtg agcttgccgc gcgtcgcgcc gtgcttctgg ttgagcttgc cgcgcagggc
      481 ttccagctcg gtgacgtcga tctcctcgac gtaggtgaag tgcgggatgc ggcgcttggc
      541 gtcctgcatc ttctgcgcga tcttgcggcg caggccgatg acggggatct gctcctcctc
      601 ggtgcgctgc gcgtagcgcg tgtcgacgct gccggacgcg ccggtgccgc cgaccaggcc
      661 gcgcgcggcg taggcctcga tgtcctcgtg cgtgatgcgg ccggcggtgc cggtgcccat
      721 cacgtactgc aggtcgatgc ccaggtccca ggcgtgcgcg cgcacggcgg gcgacgcgat
      781 gggcttgtcg gtcggggcgc gcgtgatctt ctgcgcgccg gcggccgggg ccttcgcggg
      841 cgcgggcgtc ggagccttgg cgaccggtgc aggcgccggg gcgggggtgg tcgcgacggc
      901 cggtgccggc gcgggagccg ccttcggcgc gggcgcgggg gcggcggcag caggcgccgg
      961 agcggcggcg ccgtccttga cgttgcccgc gccctcgacc tcgatgcgga tcagctcgga
     1021 gccgaccgcc atgacgtcgc cgaccttgcc gccgagcgcc agcaccttgc cgtgcaccgg
     1081 cgacgggatc tcgaccgtgg ccttgtcggt catgacgtcg gcgaggttca tgtcctcgac
     1141 gacgttgtcg cccaccttga cgttccaggc gaccagttcg acttccgcga tgccttcgcc
     1201 gatgtccggc atcttgatga cgtggatacc catcttctta tttctccatg gcgcgcttga
     1261 acgcggcgcc gacgcggtcg ggccccggga aatacgccca ctcctgcgca tgcgggtagg
     1321 gggtatccca gccggcgacg cgttcgatgg gggcttccag gtggtagaag cagtgctcct
     1381 ggacgagcgc ggcgagctcc gcgccgaagc cgctcgtctt cgtggcctcg tgcacgacga
     1441 cgcagcggcc ggtcttcttc accgacctga cgatggtgtc gaggtccagc ggccagatgc
     1501 tgcgcaggtc gatgatctcg gcgtcgatgc ccgtctcggc cgcggcggcc tcgctgacga
     1561 acaccatcgt gccgtaggtc agcaccgtca gctgcgagcc ttcgcggtag accgaggccg
     1621 agtccagcgg caccgtgtag tagccctcgg gcacctcgcc cagcttatgc ttggaccacg
     1681 gcaccacggg cttgtcgtgg tggccgtcga acgggccgtt gtacaggcgc ttgggctcca
     1741 ggaagatcac cgggtcgtcg ttctcgatgc acgagatcag caggcccttg gcgtcgtacg
     1801 ggttggacgg catcaccgtg cgcaggccgc acacgtgcgt gaacatcgcc tcgggcgact
     1861 ggctgtgcgt ctggccgccg tagatgccgc cgccgcaagg catgcggatc gtcatcggcg
     1921 cggtgaagtc gccggccgag cggtagcgca ggcgcgccgc ctcggacacg atctggtcgg
     1981 acgccgggta gaagtagtcc gcgaactgga tctcgacgac cggacgcagg ccgtaagcgc
     2041 ccatgcccac ggccgcgccg acgatgccgc cctcggaaat cggcgcgtcg aacacgcgct
     2101 gcgcgccgta cttggcctgc aggccttcgg tgcagcgaaa cacgccgccg aagtagccga
     2161 cgtcctcgcc gaacacgacg acgttcggat cgcgcgccag catcacgtcc atggccgaac
     2221 gcagcgcctg gatcatcgtc atcggggcgg tcgggccttg gggctgggcc gccagagtct
     2281 tcttgtcttg catttccatg atcagagtcc ttc
//
LOCUS       DATCRO010000500         5278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_331808, whole genome shotgun sequence.
ACCESSION   DATCRO010000500 DATCRO010000000
VERSION     DATCRO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331808"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VIN75_13650"
     CDS             <1..693
                     /locus_tag="VIN75_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975262.1"
                     /translation="ETGPAPEPVRSGYVAIAISDDGSGMPEDVRERAFEPFFTTKEAG
                     RGTGLGLSTVYGFAKQSRGTVTLASRPGLGTTVTLFIPQAQAPAAETPDEASDGATLP
                     AGLSVLLVEDEAEVRKVALAFLKAQGARVRECVDAAEAMAALDADASFDLLLSDIALG
                     TGMRGTELARVLQERQPGLRVLLVSGFSSELLDADQTAPPDWALLPKPYSRAELVRAI
                     VAALRRDGEPRA"
     gene            907..1365
                     /locus_tag="VIN75_13655"
     CDS             907..1365
                     /locus_tag="VIN75_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975263.1"
                     /translation="MPPEADPANVLVKRARALRASLCDPAVTPLKGRHVAIAAAGHDT
                     PSARRFERAATALGARVSHIGPESLVLDDARLATTARILGSLYDAIDFIPMPAERADF
                     LQRLAGVPVFADLGGELSPLRELLTPATDEAEAEAELLALMEAVLLRAVG"
     gene            1483..2100
                     /locus_tag="VIN75_13660"
     CDS             1483..2100
                     /locus_tag="VIN75_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY8975264.1"
                     /translation="MLRSHRLILAALAAFPLIAGAQARDDSGDWIVRLRVTYLQSANK
                     DDTGLGLSINDKTLPELDVTRFFTPNLAAELVLTVPQKQTISAGGTPIGSLKHLPPAL
                     LLQYHVTGLGALRPYVGAGVNWTHFSDVQFDAATTAALHPSIKKDSFGAALQAGLDVP
                     VGRGWLLNADAKWVQIKTDIESSGAKVGTFKVNPWLLSVGAGYRF"
     gene            2313..3161
                     /locus_tag="VIN75_13665"
     CDS             2313..3161
                     /locus_tag="VIN75_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8975265.1"
                     /translation="MSYPTILLLLEDDPHADTRNEAAIEFALRCGAHLHGLACHLAVP
                     TGPEFAVAPGGPNDPVRRDLEAGERQAVERARRFARSCERFGVASFETSLDDTGEPAH
                     AILRHAPLHDLVLLGQPDPLDPDVHRRNAVVEDIVLRNPRPTLLYPSAGDFRQFARSA
                     LIAWDGSRGAARAVADALPLLQRCQSVRVAQFDRGSAQFPASGQSPPAGLERWLRRHG
                     IEAHAGVHYTAGDIGVALLSHAGDVGADLLVMGSWGHGPWRERLLGGVTRTVLANMRV
                     PVITSH"
     gene            complement(3343..4680)
                     /locus_tag="VIN75_13670"
     CDS             complement(3343..4680)
                     /locus_tag="VIN75_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY8975266.1"
                     /translation="MKTIPMHPVDAAWFHMDGAENTAVVTALLTSRRRFDAAAVRSQL
                     GRRLAPLPQFHRKVADSQTWTGAPAWIESPSIDIDQHVHHRALPEGAKQAQLLELVED
                     LASQPLPMGMPLWQAHVIDGPGAAGAIVFRYHHCLGDGMAMVGVASSIMDGGDDFAIA
                     ALPGAQGTPASALDAAWRLAGTALEGSFDIVRDLVRPSDAATALKAPASARQRVGWSR
                     PLPLDRVKAIARAFDAKVNDVAVAAIAGALRRHLPASCRAPATIRAMVPVNLRATTDI
                     GDASNGFGLAILELPVDEADAAERLRKATRRMSAIKHSAEATAMHELFDLFGRGPKAL
                     QQVAQGLFEDKVSLVLTNVRGPAAPLKLAGRTIERFIFWVPHPGERVGLGISVCSYRG
                     RIAVGVLADALKLPDAQGLARRFETEIAALERAARALESPARTKKPARTADRV"
     gene            complement(4949..>5278)
                     /locus_tag="VIN75_13675"
     CDS             complement(4949..>5278)
                     /locus_tag="VIN75_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEY8975267.1"
                     /translation="PSGFEVVVMVSPVWCWRLSPPMRSFARSMHGKLGNVAVVSCMGG
                     SGASNAVAEIERLAHRKAVATLALRQDQVEAGRHAEPLQAFADEVAAFAAAHARPKAA
                     IATAKAA"
BASE COUNT          751 a         1879 c         1842 g          806 t
ORIGIN      
        1 gagacagggc cggcgccgga gcccgtccgc tcggggtatg tcgcgatcgc catctccgac
       61 gacggttccg gcatgcccga ggacgtgcgg gaacgggcgt tcgagccgtt cttcacgacc
      121 aaggaggccg ggcgcggcac cggcctggga ctgagcaccg tctacggctt cgccaagcag
      181 tcgcgcggca cggtgacgct cgcctcgcgg cccgggctgg gcaccaccgt cacgctgttc
      241 atcccccagg cgcaggcgcc cgcggcggag acgccggacg aggcgtccga tggggcgacg
      301 cttcccgccg ggctgtccgt cctgctggtg gaggacgagg ccgaggtgcg caaggtggcg
      361 ctcgccttcc tcaaggcgca gggcgcccgc gtgcgcgaat gcgtcgatgc ggccgaggcc
      421 atggccgccc tcgacgccga tgcgtcgttc gacctgctgc tgtccgacat cgcgctcggt
      481 accggcatgc gggggacgga actcgcacgc gtcttgcagg aacggcagcc ggggttgcgc
      541 gtgctgctgg tgtcggggtt ctccagcgaa ctgctggacg ccgaccagac cgcgccgccg
      601 gactgggcgc tgctgcccaa gccgtactcg cgcgccgagc tggttcgggc catcgtcgcc
      661 gcgctgcgcc gggacggcga gccccgggct tgacgcaggt caaggttcgc accggttcgc
      721 gacactggcc gggcgagatc gtgtcgactc ctggcgactg ccgggccatt gcgtgagcat
      781 gagcccattg ccttgaccga tcgcaaggcg cacggacgaa gcgcccctaa cctgtcgtcg
      841 cggggcggtc tcccgcttcg cccacagcca tcgtccatgc cccccgtccc tgaaatgcct
      901 gagcccatgc cgcccgaggc tgacccggcc aacgtgctgg tgaagcgggc ccgcgcgctg
      961 cgtgccagcc tgtgcgatcc cgccgtcacc cccttgaagg gccgacacgt cgccatcgcg
     1021 gcggcgggac acgacacgcc gtccgcgcgc cggttcgaac gggcggcgac cgccctgggc
     1081 gcacgggtgt cgcacatcgg cccggagtcg ctcgtgctgg acgacgcgcg cctggcgacc
     1141 accgcgcgca tcctgggcag cctctacgac gcgatcgact tcatcccgat gccggcggag
     1201 cgtgccgatt tcctgcagcg cctcgcgggg gtgcctgtct tcgcggacct gggcggcgag
     1261 ctgagcccct tgcgcgaact gctgacaccg gcgacggatg aggccgaagc ggaggccgaa
     1321 ctcctcgcgc tgatggaggc ggtgctgttg cgcgccgtgg gctgatgcgg tggccgacgt
     1381 tgcgtcgtgc cgacgccaga gcgcttgttg tcgcaggaca aggcggcatg ggcgaccgcg
     1441 cgctgcaatg agatccttca taacagcaga cgacatcgca tcatgcttcg ctctcatcgc
     1501 ctcatccttg cagccttggc tgccttcccg ctgatcgccg gcgcgcaagc gcgcgacgat
     1561 tccggcgact ggatcgtgcg cctgcgcgtc acctacctgc agtcggcgaa caaggacgac
     1621 accggcctcg gcctgtcgat caacgacaag acgctgcccg agctggacgt cacccgcttc
     1681 ttcacgccga acctcgccgc cgagctggtg ctgacggttc cgcagaagca gacgatctcc
     1741 gccggcggca cgccgatcgg ctcgctcaag cacctgccgc cggcgctgct tctgcagtac
     1801 cacgtcaccg gcctcggtgc gctgcgtccc tacgtcggcg ccggcgtcaa ctggacgcac
     1861 ttctccgacg tgcagttcga tgcggccacg acggcggcgc tgcacccgtc gatcaagaag
     1921 gacagcttcg gtgcggcgtt gcaggccggg ctcgacgtgc ccgtcggacg cggatggctg
     1981 ctcaacgcgg atgcgaagtg ggtgcagatc aagacggaca tcgagtcctc gggcgccaag
     2041 gtcggcacct tcaaggtcaa tccgtggctg ctgtccgtgg gggcgggcta ccggttctga
     2101 agttgctccg tggaaacgcg cgatttcgcc gttgcgcgtc gcgggttgcg cgcgctcaac
     2161 gtcgagcacc gcgtggattg ccaagttgca ccgagcttcg tcctgatgag ccgacgccgg
     2221 aaaccggcca ccccgacccg ggccaggatg tcgacgacat cggcccggcc atccagcacg
     2281 aggccgctcg ccccgccaac cgtggagtcc tcatgagcta tccgacgatc ctgctgctcc
     2341 tggaagacga cccgcatgcc gacacgcgca atgaagcggc gatcgagttc gcgctgcgtt
     2401 gcggcgcgca tctccacggc ctggcctgcc atcttgccgt tccgaccggc ccggaattcg
     2461 ccgtggcgcc gggcgggccg aacgacccgg tcaggcggga cctggaagca ggcgagcggc
     2521 aggccgtcga gcgtgcccgg cgattcgcac gatcctgcga gcgtttcggt gtcgcatcgt
     2581 tcgagacctc gctggacgac accggcgagc cggcgcacgc catcctgcgt cacgcgcctc
     2641 tgcacgacct ggtgctgctc ggccagcccg atcccctgga tccggacgtc caccggcgca
     2701 acgcggtcgt ggaggacatc gtgctgcgca atccgcgtcc gacgctcctc tatccgtctg
     2761 cgggggattt ccggcaattc gcccggagtg ccctgatcgc ctgggacggc agccgcggcg
     2821 cggcccgggc cgtcgccgac gctctgcctc tgctgcagcg ctgccaaagt gtccgcgtcg
     2881 cccagttcga tcggggttcg gcgcaatttc cggcctccgg gcaatcgccg cctgccggac
     2941 tcgagcgctg gctccggcgc cacgggatcg aggcgcatgc tggcgtgcac tacaccgccg
     3001 gcgacatcgg cgtcgcgctg ctgtcccacg caggcgacgt gggcgccgac ctgctcgtga
     3061 tgggcagctg gggccatggg ccctggcggg agcgactgct gggcggcgtg acgcgcaccg
     3121 tgctggcgaa catgcgagtg cctgtcatca cctcgcattg aacgacgacc gctgcgtctt
     3181 caaggctggt cgcctgcttc gcaggggtct gcggtggtcg tcgcccggac ggcgcgaaaa
     3241 cgacaggaag atgctgcctg gggcgacttg atcgtcgtca atcgaccggc gggcagatcc
     3301 gatccctctg ccttccagga ccatcgatcg ttcggttgcc ggtcagactc gatcggccgt
     3361 ccttgcgggc ttcttcgtcc gagccggcga ttccagcgcg cgagctgcgc gctccaaggc
     3421 agcgatctcg gtctcgaacc ggcgggccag gccttgcgcg tcgggcagct tcagcgcgtc
     3481 ggccagcacg ccgacggcga tgcgtccgcg atagctgcac acgctgatgc ccaggccgac
     3541 gcgctcgccg gggtggggca cccagaagat gaagcgctcg atcgtccgcc ccgcgagctt
     3601 cagcggcgcc gctggcccgc gcacgttcgt cagcaccagg ctgaccttgt cctcgaacag
     3661 gccttgtgcg acctgctgca acgccttggg cccgcgcccg aagaggtcga acagctcgtg
     3721 catggccgtc gcctcggccg aatgcttgat cgccgacatg cgccgggtcg ccttcctcaa
     3781 gcgctcggcg gcgtccgcct cgtcgaccgg gagctcgagg atggcgagcc cgaagccatt
     3841 gctggcgtcg ccgatgtccg tcgtcgcgcg caggttcacg ggcaccatcg cccggatcgt
     3901 ggcgggcgcc cggcacgacg cggggagatg gcggcgcagc gcacccgcga tcgccgcgac
     3961 ggcgacgtcg ttgaccttgg cgtcgaacgc ccgggcaatg gccttgacac ggtcgagagg
     4021 caggggccgc gaccagccca cccgttgccg ggcgctggcg ggcgccttga gggccgtcgc
     4081 cgcgtcagaa ggcctcacca ggtcgcggac gatgtcgaag ctgccttcga gcgcggtgcc
     4141 cgccagccgc caggcggcgt ccaatgccga tgctggtgtg ccctgggcgc ccgggagcgc
     4201 tgcgatcgcg aagtcgtcgc ctccatccat gatgctgctc gcgactccga ccatcgccat
     4261 gccgtcgccc aggcaatggt gatagcggaa gacgatggcg cccgctgcgc cgggcccgtc
     4321 gatcacgtgc gcctgccaca gcggcatgcc catgggcagc ggctggctcg cgagatcctc
     4381 gacgagttcg agcaactgcg cttgcttggc gccttcgggc aaggcacggt gatgcacgtg
     4441 ctggtcgatg tcgatcgatg gcgactcgat ccaggccggt gcgccggtcc acgtctgcga
     4501 gtcggcaacc ttgcgatgga actgcggcag tggcgcgagc cggcggccca gctggctgcg
     4561 cacggcggcg gcgtcgaacc ggcgccgcga ggtcaacaag gcggtcacga ccgccgtgtt
     4621 ctcggcgccg tccatgtgga accaggcggc gtcgaccgga tgcatcggga tcgtcttcat
     4681 gatgatgtcg accttagccg cgggaggccg acgacacgtt gcgcgggcgc aagcggcgcc
     4741 gccgcgctga agtgcacatg ggaactgcgc cgaagattgc ggccgcgcaa cgccctgtcg
     4801 cgcacggagc cgcaggctgc cgagatgacc tcgaatgcgc agcctcggcg gctcgtcctt
     4861 gacgacccgg gttgccgcac ggcccgggat gccggcgtgc tcgtgaacaa ggtcgtcaac
     4921 gagatcctgc tgcagccctg acgccccttc aggccgcctt ggccgtggcg atcgccgcct
     4981 tcggccgggc atgcgccgcg gcgaatgcgg cgacctcgtc ggcgaacgcc tgcaacggtt
     5041 cggcatgccg gccggcctcg acctggtcct ggcgcagcgc gagcgtggcc acggccttgc
     5101 gatgggcgag gcgctcgatc tccgcgaccg cgttggaagc gccgctgccg cccatgcacg
     5161 agaccaccgc cacgttgccc agcttgccgt gcatggagcg cgcgaagctg cgcatcggcg
     5221 ggctgaggcg ccagcaccac accggcgaga ccatcaccac cacctcgaag ccggaggg
//
LOCUS       DATCRO010000501         5734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1062740, whole genome shotgun sequence.
ACCESSION   DATCRO010000501 DATCRO010000000
VERSION     DATCRO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062740"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VIN75_13680"
     CDS             complement(<1..422)
                     /locus_tag="VIN75_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975268.1"
                     /translation="MPPRSSVSPVPFTLHAVALVALAVLAACSRQGTAPPPGAMAAAS
                     DAAASDAMAPLPAPMQAPAAVATQAPVQPLPQAPAQAPARTPARVAANDYSNADAPLD
                     YAPPAAGYTNESTYANAPVYADDPDYADDGQQQVVSVYV"
     gene            552..1268
                     /locus_tag="VIN75_13685"
     CDS             552..1268
                     /locus_tag="VIN75_13685"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY8975269.1"
                     /translation="MHAEDFHSPDVARLRADLALALRAAAHHGLAEGICNHFSVELPD
                     GSNRFLLNPRGLLWSEIQADDVVLIANDGTKLAGRHEVEATAMFIHAAVHRLGKPCVL
                     HTHMPYATALTLTADRALDTTLSQNAMRFHGRIAVDAHYNGLALDDAEGERIARAMGT
                     ADVAFLGNHGVIVCGERLAHAYDDLYFLERACLHQVLAQSTGRPLAPVDEALARHVGE
                     QTRGERLQSELFFEALRRTL"
     gene            complement(1287..2456)
                     /locus_tag="VIN75_13690"
     CDS             complement(1287..2456)
                     /locus_tag="VIN75_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975270.1"
                     /translation="MTFTLILAAWLALNVLATRRVLALGEHCHMPRMRIAMVWIVPFI
                     GAGMTLVDTLPDVRWMRAEAAEPPVHVQEPAPDEVPAMGVEAFPLLRHMQAVNGYPTV
                     DWRAAEAWLTSIADGAGRVAARVDLHRAWLEHLCDALGEHAWLHESDDALIVSSLETP
                     VAAAMGRYVASTRKRVAQTLGALAQFPDGVKSVVLVLDDEESYYHYISMFGPAEGEQA
                     FSGGVFVDAGCPHFVVRRDDLSAIEPTIAHELTHSALSHLRLPLWVDEGLAVNTEHRI
                     AGAHRGQFTARELHDKHVAFWDGERIQQFWTGASFRRPDDGNLLSYDLARIMVAQMGR
                     AWPAFERFARAARRVDAGADAAREHLDLDLGAYVSLLFGHEPSAAWTPGAPAGPA"
     gene            2536..2958
                     /locus_tag="VIN75_13695"
     CDS             2536..2958
                     /locus_tag="VIN75_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY8975271.1"
                     /translation="MNTTSSWQLLADAVLCSHVALMAFVVLGLPLVIVGNLRRWRWVN
                     SPWLRFAHLATILVVAGEAWAGVVCPLTTLEMWLRAQAHATTYEGSFVEHWLQALLFW
                     HAPAWVFTAAYSLFGVSVLATWWLWPVRFGRVRAAARA"
     gene            3362..3862
                     /locus_tag="VIN75_13700"
     CDS             3362..3862
                     /locus_tag="VIN75_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY8975272.1"
                     /translation="MNRTLQQGFTLIELMIVVAIIGILAAVALPAYQTYTVKAKMSEV
                     VLAASQCRTTVTEVFQTGSSAPGGGNWGCESTSATSKYVASVTTDGNGTITVKVSSDT
                     SLPTTITGDTLTLVPEDSTGAVYAVGTAGVIGSTQVYKWVCGSTAAPGATSSNLVKYL
                     PGSCRG"
     gene            3990..5081
                     /locus_tag="VIN75_13705"
     CDS             3990..5081
                     /locus_tag="VIN75_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8975273.1"
                     /translation="MIELSVVVPTYNERPNVGELVRRLDAALQGVRWEAVFVDDDSPD
                     GTADAVRALAQADPRVRCIQRLGRRGLSRAVIEGVLSTAAPVFAVIDADLQHDETVLP
                     AMLARLRGEGGQGAMDVVVASRYTAGGSLEGWDAERARMSRFATALANRMVPTGLTDP
                     MSGFFLMRRDTFDQAVRHLSGEGYKLLLDILASAPGAVRLAEVPYAFRPRHAGESKLD
                     SAVLWEFSLLLIDKKVGRWIPARFLLFSLVGISGVFVHFAVLSLLYMLGHQAFTTSQA
                     VATIVAMTSNYALNNSLTYRDQRHRGLGWWGGLLTFYAICGAGVVANVGVAGFLFERQ
                     GWALAAAAGALVGTVWNYAATRVVTWRAR"
     gene            5078..>5734
                     /locus_tag="VIN75_13710"
     CDS             5078..>5734
                     /locus_tag="VIN75_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975274.1"
                     /translation="MSALTPDQSRFRTATVAAVVLGVLAVLVLNVVPTANNDVWLLMK
                     VGQVIVDTGHIPETLLFPFTTVRENHFNAHEWLVSVIFHGFDRAFGTDRLMWVIAPFA
                     LLQFGLCVLLTRRQSGSLGAALLLSLLAMLCANSRFVLRPELFALVFLVSLLLVLDRY
                     RSGSRWTTLLWTLPIAALWANCHGSFLLAPVVASLFALGEALTAARAAAGPVPARLRA
                     G"
BASE COUNT          776 a         2068 c         1998 g          892 t
ORIGIN      
        1 acgtagacgc tgaccacctg ctgctggccg tcgtcggcgt agtcgggatc gtcggcgtag
       61 accggcgcgt tcgcataggt cgactcgttg gtgtagccgg ccgccggcgg cgcatagtcg
      121 aggggcgcat ccgcgttcga atagtcgttc gcggccacgc gcgccggcgt cctcgccggt
      181 gcctgcgcgg gtgcctgcgg cagcggctgc accggcgcct gggtcgcgac ggcggccggc
      241 gcctgcatcg gggccggcag cggcgccatc gcgtcggagg ccgcggcatc ggacgccgcc
      301 gccatcgccc ccggcggcgg cgcggtgcct tgcctggaac aggcggccag cacggcgagc
      361 gcgaccagcg cgacggcgtg cagcgtgaac gggaccggac tgacggatga gcgcgggggc
      421 atgatggctg gcctctcttc gatgaaatcg tccctgcgcg gcaatcggcc tgcccggcgg
      481 gccgccggag tgcgcgccga cgaaccggcg cccttccctg tagagtcggg atctgccgcc
      541 agggagccgc catgcatgcc gaggatttcc attcgcccga cgtcgcgcgc ctgcgcgccg
      601 acctcgcgct cgcgctgcgc gccgccgcgc accacgggct cgccgagggc atctgcaacc
      661 acttcagcgt ggagctgccc gacggctcga accgcttcct gctgaacccg cgcgggctgc
      721 tctggagcga gatccaggcc gacgacgtcg tgctgatcgc caacgacggc acgaaactcg
      781 cgggccgcca cgaggtcgag gccaccgcga tgttcatcca cgccgccgtg caccgcctgg
      841 gcaagccctg cgtgctgcat acccacatgc cgtatgcgac cgcactgacg ctgactgccg
      901 accgcgccct ggacaccacg ctgtcgcaga acgccatgcg cttccacggc cgcatcgccg
      961 tcgatgcgca ctacaacggc ctcgccctcg acgacgccga aggcgagcgc atcgcccgcg
     1021 cgatgggtac ggccgacgtc gccttcctcg gcaaccacgg cgtgatagtc tgcggcgagc
     1081 gcctggcgca cgcgtacgac gacctgtact tcctcgagcg cgcctgcctg caccaggtgc
     1141 tggcgcagtc gaccggccgg ccgctggcgc cggtcgacga ggcgctcgcc cgccacgtcg
     1201 gcgagcagac gcgcggcgag cggctgcagt cggagctgtt cttcgaggcg ctgcggcgga
     1261 cgctgtagcg ccgcgcgcca gcagcttcag gccggtccgg cgggcgcccc gggcgtccac
     1321 gcggcgctgg gctcgtggcc gaacagcagg ctcacgtacg cgcccaggtc gaggtcgagg
     1381 tgctcgcgcg ccgcgtccgc gcccgcgtcc acgcggcgcg ccgcgcgcgc gaagcgctcg
     1441 aaggccggcc aggcgcgccc catctgcgcc accatgatgc gcgcgaggtc gtaggacagc
     1501 aggttgccgt cgtcggggcg ccggaacgag gcgccggtcc agaactgctg gatgcgctcg
     1561 ccgtcccaga acgccacgtg cttgtcatgc agctcgcgtg ccgtgaactg gccgcgatgc
     1621 gcgccggcga tgcggtgctc cgtgttgacc gccaggcctt cgtcgaccca cagcggcagc
     1681 cgcaggtgcg acagcgcgct gtgcgtgagc tcgtgcgcga tcgtcggctc gatggcgctc
     1741 aggtcgtcac gccgcacgac gaagtgcggg caccccgcgt cgacgaagac gccgccactg
     1801 aacgcctgct cgccctcggc cgggccgaac atggaaatgt agtggtagta gctctcctcg
     1861 tcgtcgagca ccagcaccac gctcttcacg ccatccggga actgcgcgag cgcacccagg
     1921 gtctgcgcga cgcgcttgcg ggtactcgcc acgtagcggc ccatcgccgc agccacgggg
     1981 gtctccagcg acgacacgat cagcgcatcg tcgctctcgt gcagccaggc gtgctcgccc
     2041 agcgcgtcgc acaggtgctc cagccacgca cggtgcaggt cgacgcgcgc ggccacgcgt
     2101 ccggcaccgt ccgcgatgga cgtcaaccag gcctccgccg cacgccagtc caccgtcggg
     2161 tagccgttga cggcttgcat gtgccgcagc aacgggaacg cctcgacacc catcgccggc
     2221 acctcgtcgg gcgccggctc ttgcacatgc accggcggtt cggcagcctc ggcgcgcatc
     2281 cagcggacgt cgggcaaggt gtcgaccagc gtcatgccgg cgccaatgaa cggcacgatc
     2341 cataccatcg cgatgcgcat gcgcggcatg tggcaatgct cgccgagcgc cagcacgcgg
     2401 cgcgtcgcca gcacgttgag ggccagccag gcagccagga tgagcgtgaa ggtcatggcg
     2461 cgcgatgtta gcggctgccc ggctgtcggg cgctcgccgg cacaatgcgg tcgatgagat
     2521 cgtgaaccgc gccacatgaa caccacctcc tcctggcaac tgctcgcaga cgccgtgctc
     2581 tgctcgcacg tcgcgctgat ggccttcgtc gtgctcggac tgccgctggt gatcgtcggc
     2641 aacctgcgcc ggtggcgctg ggtcaactcg ccgtggctgc gcttcgcgca tctggcgacc
     2701 atcctggtcg tcgcgggcga ggcgtgggcg ggtgtcgtgt gcccgttgac gacgctggag
     2761 atgtggctgc gcgcgcaggc gcatgcgacg acctacgaag gcagcttcgt cgagcactgg
     2821 ctgcaggcgc tgctgttctg gcacgcgccg gcatgggtgt tcacggcggc gtattcgctg
     2881 ttcggggtgt cggtgctggc gacctggtgg ctgtggccgg tgcgcttcgg gcgcgtccgc
     2941 gcagctgcgc gcgcctgaca ctccggccgt cgctgtcgtc gcttgccgcc agcggcgttg
     3001 cgccggcgac aggccgggca gcagcgccgc gcctggcgcg aaccatgcgt ccgtgccgac
     3061 acgccgtctc gtgtccgacg aacgaacgga caacggcgac ctcgcccggg ccgcccgagc
     3121 cgttcccctc ggcccggtgt gccgaacttg gcatcggccg gtgcgcggtc atcgcgcggt
     3181 aaagacgccc gccgggcgcg ccggaagaat tcgacggcgc caccacaagg gcgcaggcgc
     3241 gttccgggca acggcgaacg gtcggaccgc tccttgacag cgtcgcgacc gttccccgag
     3301 gcacgggaag gcgcgttcct caacttcacc gcatggcttg tcgccacccc aggagcttcc
     3361 tatgaaccgt accctgcaac agggtttcac attgatcgaa ctgatgatcg tcgtcgcgat
     3421 catcggtatc ctcgccgccg tcgccctgcc ggcctaccag acctacacgg tcaaggccaa
     3481 gatgtcggaa gtcgtgctgg ctgcctcgca gtgccgcacc acggtgaccg aagtgttcca
     3541 gaccggttcg tcggctcctg gcggcggcaa ctggggctgc gaaagcacct cggccaccag
     3601 caagtacgtc gccagcgtga cgaccgacgg caacggcacg atcaccgtca aggtctcgag
     3661 cgacacctcg ctgccgacca ccatcaccgg tgacaccctg acgctggttc cggaagactc
     3721 caccggcgcc gtctacgctg tcggcaccgc cggcgtcatc ggcagcacgc aagtctacaa
     3781 gtgggtctgc ggctcgaccg ctgccccggg cgcgacctcg tcgaacctgg tcaagtacct
     3841 gccgggttcg tgccgcggct gattcgggct tgccctgaag atcggaaaga ggtgcttcgg
     3901 cacctctttt tcattggagg gtgcgaatcc gcgcaccggg cggcgtgccg gaaccgacga
     3961 caatgggcga ttcgcggact ggagtgcaga tgattgaact gtcggtcgtt gttccgacct
     4021 acaacgagcg gcccaatgtc ggcgaactgg ttcgacgcct cgacgcggcg ctgcagggtg
     4081 tccgctggga agccgtgttc gtcgacgacg attcccccga cggcaccgcc gacgccgtgc
     4141 gtgcgctggc ccaggccgat ccgcgcgtgc gctgcatcca gcggctcggc cggcgcggcc
     4201 tgtcgcgcgc ggtgatcgag ggcgtcctga gcacggcggc cccggtcttc gccgtcatcg
     4261 acgccgacct ccagcatgac gagaccgtgc tgcccgcgat gctcgcgcgc ctgcgcggcg
     4321 agggcggcca gggcgcgatg gacgtcgtcg tcgccagccg ctataccgcc ggcggcagcc
     4381 tcgagggctg ggacgccgag cgcgcgcgca tgagccgttt cgccaccgcg ctggccaacc
     4441 gcatggtacc cacgggcctg accgacccga tgagcggctt cttcctgatg cgccgcgaca
     4501 ccttcgacca ggccgtgcgc catctctccg gcgagggcta caagctgctg ctggacatcc
     4561 tggcctccgc ccccggcgcg gtgcgcctgg ccgaggtgcc gtacgcgttc cggccgcgcc
     4621 atgccggcga gagcaagctc gactcggcgg tgctgtggga attctcgctg ctgctgatcg
     4681 acaagaaggt cggccgctgg atcccggcgc gcttcctcct gttctcgctg gtgggcatct
     4741 ccggcgtgtt cgtgcacttc gcggtgctga gcctgctgta catgctgggg caccaggcgt
     4801 tcacgacgtc gcaggcggtg gccaccatcg tcgcgatgac gtccaactac gcgctcaaca
     4861 actcgctgac ctaccgcgac cagcgccacc gcggcctggg ctggtggggc ggcctgctga
     4921 ccttctacgc gatctgcggc gcgggcgtcg tcgccaacgt gggcgtcgcc ggcttcctgt
     4981 tcgagcgcca gggctgggcg ctggccgcgg ccgccggcgc gctggtgggc accgtctgga
     5041 actacgccgc cacgcgcgtc gtcacctgga gagcccgttg agcgcgctga cgccggacca
     5101 atcccgtttc cgcacggcga cggtcgccgc ggtggtcctg ggcgtgctgg ccgtgctggt
     5161 gctcaatgtc gtgccgacgg ccaacaacga cgtctggctg ctgatgaagg tcggccaggt
     5221 gatcgtcgac acgggccaca tccccgagac gctgctgttc ccgttcacca cggtgcgcga
     5281 gaaccacttc aacgcgcacg agtggctggt gtcggtcatc ttccacggct tcgaccgcgc
     5341 gttcggcacg gaccgcctga tgtgggtgat cgccccgttc gcattgctgc agttcggcct
     5401 gtgcgtgctg ctgacccggc ggcagtcggg cagcctgggc gcggcgctgc tgctgtcgct
     5461 gctggcgatg ctctgcgcga attcccgctt cgtgctgcgg cccgagctgt tcgcgctggt
     5521 gttcctcgtg tcgctgctgc tggtgctcga tcgctaccgc agcgggagcc gctggacgac
     5581 gctgctgtgg acgctgccga tcgccgcgct gtgggccaac tgccatggct cgttcctgct
     5641 ggcgccggtg gtggcgagcc tgttcgcgct cggggaggcg ctcaccgccg cgcgtgccgc
     5701 ggccggcccc gtgcccgcgc gcctgcgcgc cggc
//
LOCUS       DATCRO010000502        11205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_117926, whole genome shotgun sequence.
ACCESSION   DATCRO010000502 DATCRO010000000
VERSION     DATCRO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117926"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1546
                     /locus_tag="VIN75_13715"
     CDS             176..1546
                     /locus_tag="VIN75_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEY8975275.1"
                     /translation="MTTSPYDALVIGTGQAGPTLAVRLAGHGWRTALVERGPFGGTCV
                     NNGCTPTKTLVASARAAWVARHAADFGVTIAGPVGIDYAAVRDRMMKVVQASRDGLGR
                     WFEGTANLDVIHGQARFTGRDTMQVGDRVLQAPRIFLNTGARPVVPDWIAASGAPFLT
                     SESLMGLEELPTRLVILGAGYIALEYAQVYARFGCDVTVIEHGPRLLPREDDEAARVI
                     QAVLERDGVRFCFGANATALAREGAGIALTVDGRRLAGSHLLVAIGRRPDTEALDLAR
                     AGVTCDARGHVRVDDQLRTDVPGIWALGDVNGRGAFTHTSYNDFEIVAANLLDDDPRR
                     VTDRVPAYALYTDPPLARIGMARAEAKASGRRVLVGHLPMSRVGRARERGETDGYLEA
                     LVDADDQRLLGATLVGIEADEAIHGLLDVMSAGLPYTAISRTMHIHPTVSELIPTMLQ
                     SLEPLE"
     gene            1543..1827
                     /locus_tag="VIN75_13720"
     CDS             1543..1827
                     /locus_tag="VIN75_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8975276.1"
                     /translation="MSSPQVFAVTLLPSGSVFEARADESLLKAGLRAMVSMRSSCRNG
                     TCRTCLCRLQSGSIAYEIEWPGLTREEKDEGLVLPCVARPLSDIVLRYDV"
     gene            complement(2012..2749)
                     /locus_tag="VIN75_13725"
     CDS             complement(2012..2749)
                     /locus_tag="VIN75_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8975277.1"
                     /translation="MLSLVLVAGLWAGLQNSLAGGGSFITLPALIVSGMSPLAANITS
                     TVALFPGQVTSGLAGRSLVSGVGRLTFRWLFALSLAGGAVGGLLLLNTPPTVFARLVP
                     WLVLFATTVFAWGSFRRKPLEGSAHIGPAAAGFAQFLIGIYGGYFGGGIGFLMLAALT
                     LAGLSTRNAGATKNALASVMNAAAVVLFVFSPQVHWLQAAALGAGAIVGGLAGAWALK
                     RVNERALRIAIVCIGVALTIGLFLRPI"
     gene            2877..3872
                     /locus_tag="VIN75_13730"
     CDS             2877..3872
                     /locus_tag="VIN75_13730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8975278.1"
                     /translation="MTLQRRKLGSQGLEVSAIGLGCMGMSQSYGPADEAESIATLHRA
                     IELGCNFLDTAEVYGPLTNEALLGKALKGRRHEVTIATKFAWKFKDGKQIPGARASTP
                     GEIREALEGSLRRLGTDHVDLLYQHRIDPAMPVEEVAETVGRFVKEGKVRFFGLSEAG
                     VANIRRAHAVHPVTALQSEYSLWERNLEAEIIPTLRELGIGLVPFSPLGRGFLTGEVQ
                     RAEDLPEDDFRHDDPRYQGANFDANVAAAAKVREIAERLGATAGQVALAWVLAQGDDI
                     VPIPGTKRRKWLEQNVAAADLRLGPDVLAALGAALAPGKVQGPRYSPERMAMVDR"
     gene            complement(3924..4676)
                     /locus_tag="VIN75_13735"
     CDS             complement(3924..4676)
                     /locus_tag="VIN75_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8975279.1"
                     /translation="MPVRIRPLITTVALAAAVPASFAAHPLQTEDTGTQGAGNLEIEN
                     GLSRTRYASTTQTVYQPQLSLGLATTLDAIVQPSYAWQHVAQERASGLGDTNVDAKWR
                     FWDSHPLSLAIRGGVALATNQDGLGLTHGKSSEHVKLALTWDRSPTTVHVNVDTTFVP
                     RAPASPARRVMTGVSAAVMQQVDEHLILTVDTGFAQSPNPHKASWPGTVLAGVIWTVR
                     PGLDLDLGWQRSVDDTPVARTWLAGLTYRFAL"
     gene            4799..5794
                     /gene="pstS"
                     /locus_tag="VIN75_13740"
     CDS             4799..5794
                     /gene="pstS"
                     /locus_tag="VIN75_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY8975280.1"
                     /translation="MFEFPRPWLMLALAWACNAQAAPALRGAGATFPAPVYEAWAAAY
                     ERTTGTSVRYDPIGSGLGIELATSGQADFGATDVPLPADRLAAAGLRQFPAVIGAVVP
                     VVNIAGIAPGRLVLDAPVLSAIYRGRVTAWSDPAIAALNPGLALPATRITVIHRADAS
                     GSTWLWSRWLSDADPAWRDTLGAGATLAWPVGTEGLGNEGVASLVQRTRSSIGYVAYA
                     YAREHRLSDVRLPSRDGPVLRAERPTFEAAVAAARAHGDPDLSDALTNEPGPRSWPIV
                     GASYVLVRRDGPQAARVDAFFAWALGSGAAMVDGLDYLPLPADAAVRVLATLRHD"
     gene            complement(5974..7635)
                     /locus_tag="VIN75_13745"
     CDS             complement(5974..7635)
                     /locus_tag="VIN75_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8975281.1"
                     /translation="MNAATVPTLPIDGKSHVVGRTDLPLSDATIYGLLADTAKCYPDR
                     LAVVFREQGVRWTWREFLAEVDAFGAGLSALGLAKGDRVGIWSPNRVEWLVTQFATAR
                     MGLVLVNVNPAYRLSELTYALQVSGCRAIVSAERLKTSMYLEMLQKVRRELPALEHVI
                     RMGGERSPGMLNYADVLERGRGAPLPFADCLCHDAINIQFTSGTTGNPKGATLTHHNV
                     VNNGRFIAMAMNFSDKDSLCIPVPLYHCFGMVLAVLACVSTGAAMVFPGEAFDPAATL
                     SAVAEERCTALHGVPTMFIAELAHPSFAQYDLRSLRTGIMAGSPCPIETMKQVVSRMN
                     MREVTIAYGMTETSPVSFQSSTADPLDRRTTTVGRVQPHLECKIVDADGNIVPVGEKG
                     ELCTKGYSVMLGYWGDDKRTAEAVVDGWMHTGDLATIDEEGYCNIVGRVKDMLIRGGE
                     NVYPREIEEFLFRHPKVQSVQVFGVPDAKYGEEVCAWIVLKAGEASTEDEIRDFCRDQ
                     IAHYKVPRYIRFVDDLPMTVTGKAQKFVMRDRMIEELKLQRTQTA"
     gene            complement(7829..8404)
                     /locus_tag="VIN75_13750"
     CDS             complement(7829..8404)
                     /locus_tag="VIN75_13750"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY8975282.1"
                     /translation="MGNERRKAAPPALMGLAVAAAALAPAAAGAKELTSPYGVFIEGG
                     ISDDSRSVVGGVAWTWVQPSKWGAGNASGYLETSIGEWACHREAGEQYHACSTQIGLT
                     PVVRYEPSWWEHWYMELGIGVDVIAPAWKTDRRRFGSDFNFGDHVGLGRKFGDEGRDE
                     IELRAEHYSNGGIKHPNPGENWFQLRYMHWF"
     gene            complement(8497..9279)
                     /locus_tag="VIN75_13755"
     CDS             complement(8497..9279)
                     /locus_tag="VIN75_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8975283.1"
                     /translation="MSLIDIGVNFHSSQLKGKDAELLSRAAAAGVTHILATGTSLLAS
                     ELALAFARTHARVWATAGVHPHDARHWTSHSPAQFEPLWADERVVAVGECGLDYNRMF
                     SPMQDQRAAFEAQIAAAVRLDKPLFLHCRDAFDDFHAMLRDAAAAGAHGVVHCFTEGP
                     AQAEAFLALGFDIGITGWVTDETRGSALRDAVRAVPAGRLHLETDAPYLRPKNAGKTR
                     PYNEPALLPFVARAVAELKGMDVAALSLQASANSRRLFKLPD"
     gene            complement(9326..>11205)
                     /locus_tag="VIN75_13760"
     CDS             complement(9326..>11205)
                     /locus_tag="VIN75_13760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY8975284.1"
                     /translation="YRLFRHVPLVAAAVDTWAAADEDIVRLQRLGEQIHQARNAGDDD
                     AWRPRFEAEMYRLDDALNQRELLFSRQLGQAARWVQGLLLLATAGLALALIAGGLMFA
                     RQMWKQLAQSQQEVEDGNARWSLAADAGRLGLMEWRVRDDTILLDPHAIAIYELPPAP
                     GNVYKARDLRLRVHPDDRPRMKQESLNAIAARTPVVQRFRVVLPSGRMRTLEIDGRAF
                     MADGRTVSHMVGLVRDVTDEVNAQNAMLEEGAAQRTYQAKREFLSRVSHELRTPLNAV
                     IGFSDLMRTDRDEPLTATQRGRLDRVVDGGQYLLRLVNDLLDVARLEVGELSVALSSV
                     RLAPVLRNAVDLLETARAGRDVSIDLDEPPSQLFVVADPVRLKQVFVNILSNAIKYNR
                     RGGRVYIGFSLTPTQAVLTVRDEGAGMSAQQQRDLFIPFRRLGDEPNATEGAGLGLVI
                     SRHLAERQGGSLDIASAPGKGTTVTIRLNRDLDEDVEHTDFVATTAFGQGTAGAAGTI
                     VYIEDDPVNILLLEQYVALTSDVQLLVARDGESGLELARRWMPNLILLDMQLPDMSGL
                     DVLDRLRADPVTSGLRVVALSADAMPAQVTEAIARGAEAYWTKPLALSKFKSELPKFL
                     R"
BASE COUNT         1605 a         3990 c         3998 g         1612 t
ORIGIN      
        1 cggtgacggt gatcgagcac ggcccgcgcc tgctgccgcg cgaggacgac gaggccgcgc
       61 aggtgatccg cgcggtgatg ttcgtgtccg ccgccctggc cgccgcgagc gccgcgtgcg
      121 cctggtggct catcgacgac cgccccgccg ccaacgattc gaaaggaccc gcgccatgac
      181 gacctccccc tacgacgcgc tggtcatcgg caccggccag gccggcccga cgctggcggt
      241 gcgcctggcc gggcacggct ggaggaccgc cctcgtcgaa cgcgggccat tcggcggcac
      301 ctgcgtcaac aacggctgca cgcccaccaa gacgctggtg gccagcgcgc gcgccgcgtg
      361 ggtggcgcgc cacgcggccg acttcggcgt gacgatcgcc ggcccggtgg gcatcgacta
      421 cgccgccgtg cgcgaccgca tgatgaaggt ggtccaggcc tcgcgcgacg gcctgggtcg
      481 ctggttcgaa ggcaccgcca acctcgacgt gatccacggg caggcccgct tcaccgggcg
      541 cgacacgatg caggtcggcg accgcgtgct gcaggcgccg cgcatcttcc tgaacaccgg
      601 cgcgcggccc gtcgtgcccg actggatcgc cgcgtccggc gcgccgttcc tgaccagcga
      661 atcgctgatg ggcctggagg agctgcccac gcgcctcgtg atcctgggcg ccggctacat
      721 cgcgctcgaa tacgcccagg tgtatgcgcg cttcggctgc gacgtgacgg tgatcgagca
      781 cggcccgcgc ctgctgccgc gcgaggacga cgaggccgcg cgagtcatcc aggcggtgct
      841 tgagcgcgac ggcgtgcgct tttgcttcgg cgcgaatgcc actgcgctgg cgcgcgaggg
      901 cgccggcatc gcgttgaccg tcgacggacg gcgcctggcc ggcagccacc tgctcgtggc
      961 catcggccgc cggcccgata ccgaggcgct ggacctcgcg cgcgcaggcg tcacgtgcga
     1021 cgcgcgcggc cacgtgcgcg tggacgacca gttgcgcacc gacgtacccg gcatctgggc
     1081 gctgggcgac gtcaacggcc gcggcgcgtt cacgcacacc tcgtacaacg acttcgagat
     1141 cgtcgccgcg aacctgctgg acgacgatcc gcgccgcgtc accgaccgcg tgccggccta
     1201 cgcgctgtac acggatccgc cgctggcgcg catcggcatg gcccgcgccg aggcgaaggc
     1261 gtcgggccgg cgcgtgctcg tcgggcacct gccgatgtcg cgcgtcgggc gggcgcgcga
     1321 gcgcggcgag acggacggct acctcgaggc gctggtggac gcggacgacc agcgtctcct
     1381 cggtgcgaca ctggtgggca tcgaggcgga cgaggcgatc cacggcttgc tggacgtgat
     1441 gtccgccgga ctgccgtaca ccgccatctc gcgcaccatg cacatccatc ccaccgtcag
     1501 cgaactgatc cccacgatgc tgcagtcgct cgagccgctg gaatgagctc gccgcaagtg
     1561 ttcgccgtca cactgctgcc gtcggggtcg gttttcgagg cgcgagcgga cgaatcgctg
     1621 ttgaaggcgg gcttgcgggc gatggtctcc atgcgctcgt cctgtcgcaa cgggacatgc
     1681 cgcacctgcc tgtgccggct gcagagcggc tcgatcgcgt acgagatcga gtggccgggg
     1741 ttgacgcgcg aggagaagga cgaaggcctc gtgctgcctt gcgtcgcgcg gcccttgtcg
     1801 gacatcgtgt tgcggtacga cgtctgatgc ggggcgccgc ggtgccaggt gtcgcggtgt
     1861 caggccttcg agatggggtc aggtcttgaa agttgcgcga tggggcgttt caagagcgtc
     1921 gaggcttcat cgcgcaactt tcaagacctg accccatcag tggctgaccc gtccggatcg
     1981 ctctcatccc ggcaagcgca ggctcgtcgc ctcaaatcgg ccgcaggaac aacccgatcg
     2041 tcaacgccac gccgatgcac acgatcgcga tgcgcaaggc ccgttcgttg acgcgcttga
     2101 gcgcccacgc accggcaagc cctccgacga tcgcgcccgc gccgagcgcc gcggcctgca
     2161 gccagtgcac ctgcggcgag aacacgaaca gcacgacggc cgcggcgttc atcacgctgg
     2221 ccagcgcgtt cttcgtcgct ccggcgttgc gcgtcgacag tccggccagg gtcagcgcgg
     2281 ccagcatcag gaagccgatg ccgccgccga agtagccgcc gtagatgccg atcaggaact
     2341 gcgcgaagcc ggccgctgcc gggccgatgt gcgcggagcc ttcgagcggc ttgcggcgga
     2401 agctgcccca cgcgaacacg gtggtggcga acagcaccag ccagggcacg aggcgcgcga
     2461 acaccgtcgg cggcgtgttc agcagcagca ggccgccgac ggcgccgccg gccaggctca
     2521 gcgcgaacag ccagcggaag gtcagccggc ccacgcccga aacgaggctg cggcccgcca
     2581 ggcccgaggt gacctggccg ggaaacagcg ccaccgtcga cgtgatgttg gccgccagcg
     2641 gcgacatgcc cgacacgatc agggcgggca gcgtgatgaa cgagccgccg ccggcgagcg
     2701 agttctgcag gccggcccac aagccggcga ccaggacgag ggaaagcagc atggcgcccg
     2761 cgattctgcc ctcgctgcgc aaggccccac gccctcctcg ccttccgtgc cctcgcgctg
     2821 cccgtggtgc gcgtccggca tagagtcgga ctcgaaccaa tctcgacgga gcgacgatga
     2881 cactgcaacg acgcaagctg ggcagccagg ggctggaagt ctcggccatc ggcctgggct
     2941 gcatgggcat gagccagtcc tacgggccgg ccgacgaggc cgagtcgatc gccacgctgc
     3001 accgcgcgat cgagctgggc tgcaacttcc tggacaccgc cgaggtctat ggcccgctga
     3061 ccaacgaggc cctgctgggc aaggcgctca aggggcggcg ccacgaggtg acgatcgcga
     3121 cgaagttcgc gtggaagttc aaggacggca agcagatccc cggcgcccgg gcgagcacgc
     3181 cgggcgagat ccgcgaggcg ctcgaaggct cgctgcgacg gctgggcacc gaccacgtcg
     3241 acctgctcta ccagcaccgc atcgacccgg ccatgccggt cgaggaagtg gccgagaccg
     3301 tgggccgctt cgtcaaggaa ggcaaggtgc gcttcttcgg gctgtcggag gccggggtcg
     3361 ccaacatccg cagggcgcac gccgtgcatc cggtgaccgc gctgcagagc gaatactcgc
     3421 tgtgggaacg caacctcgag gccgagatca tcccgacgct gcgcgaactg ggcatcggcc
     3481 tggtgccgtt cagcccgctg ggacgcggct tcctcaccgg cgaggtccag cgcgcggagg
     3541 atctgcccga ggacgacttc cgccacgacg acccgcgcta ccagggcgcc aacttcgacg
     3601 ccaacgtcgc cgccgcggcc aaggtgcgcg agatcgccga gcgcctgggc gcgacggcgg
     3661 gccaggtcgc gctcgcgtgg gtgcttgcgc agggcgacga catcgtgccc attcccggca
     3721 ccaagcgccg caagtggctg gagcagaacg tggcggccgc cgacctgcgc ctgggacccg
     3781 acgtgctcgc ggcgctcggc gcggcgctgg cgcccggcaa ggtgcagggt ccgcgctatt
     3841 cgcccgagcg catggcgatg gtcgatcgct gaacaggtcc tgggagtctg cgcggcagaa
     3901 gccgcctgct gccgcgcaga ctcctagagc gcgaaccggt acgtcagccc cgccagccag
     3961 gtgcgggcga cgggcgtatc gtcgacgctg cgctgccagc ccaggtcgag gtccagcccc
     4021 ggccgcaccg tccagatgac gccggccagc acggtgccgg gccacgaggc cttgtgcgga
     4081 ttcgggctct gggcgaagcc ggtgtcgacc gtcaggatca ggtgttcgtc gacctgctgc
     4141 atcacggcgg ccgacacgcc ggtcatcacg cgccgcgcgg gcgaggccgg ggcgcgcggg
     4201 acgaaggtgg tgtccacgtt gacgtgcacc gtcgtcggcg agcgatccca ggtcagcgcc
     4261 agcttcacgt gctcggaaga cttgccgtgc gtcaggccca gtccgtcctg gttggtggcc
     4321 agggcgacgc caccgcggat cgcgagcgac agcggatggc tgtcccagaa gcgccatttg
     4381 gcatcgacgt tggtgtcgcc cagcccggac gcgcgttcct gcgcgacgtg ctgccacgcg
     4441 tacgagggct gcacgatggc atcgagggtg gtggccaggc ccagcgacag ctgcggctgg
     4501 tagaccgtct gcgtcgtcga cgcgtaccgg gtgcgcgaca gcccgttctc gatctcgagg
     4561 ttcccggcgc cctgcgtgcc ggtgtcctct gtctgcagcg ggtgggcggc gaacgacgcc
     4621 ggcacggcgg cggccagcgc gacggtcgtg atcagcggac ggatgcgaac gggcaaggca
     4681 ggtctttcga aggaaggcgt cggacgggac cggcggcgcg gggcagcttg cccgtgcccg
     4741 gtgacagggg cgtgtcgatt gtcatgcggc cgccatgacg ggacgggaca ctccccccat
     4801 gttcgagttt ccccgcccat ggctgatgct ggccctcgcg tgggcctgca acgcgcaggc
     4861 cgccccggcc ctgcgcggcg ccggcgccac gttcccggcc ccggtctacg aggcctgggc
     4921 cgcggcctac gagcggacca ccggcacgtc cgtgcgctac gaccccatcg gctcgggcct
     4981 gggcatcgag ctcgccacgt cgggccaggc cgacttcggc gccaccgacg tgccgctgcc
     5041 ggccgaccgc ctcgccgccg ccggcctgcg ccagttcccc gcggtgatcg gcgccgtggt
     5101 gccggtggtc aacatcgccg gcatcgcgcc aggccggctc gtgctggacg cgcccgtgct
     5161 cagcgcgatc taccggggcc gcgtcacggc ctggagcgat ccggcgatcg ccgcgctgaa
     5221 ccccggtctc gcgctgcccg ccacgcgcat caccgtcatc caccgcgccg acgcgtcggg
     5281 ctcgacgtgg ctgtggagcc gctggctcag cgacgccgac cccgcctggc gcgacacgct
     5341 gggcgccggc gcgacgctcg cgtggccggt gggcaccgag gggttgggca acgagggcgt
     5401 ggcctcgctg gtgcagcgca cgcgctcgtc gatcggctac gtcgcgtacg cctacgcgcg
     5461 cgagcatcgc ctgagcgacg tgcgcctgcc gtcgcgcgac ggcccggtgc tgcgcgcgga
     5521 gcggcccact ttcgaggcgg ccgtcgccgc ggcgcgcgcg catggcgatc cggacctctc
     5581 ggacgcgctt accaacgagc cgggcccgcg cagctggccc atcgtcggcg cgagctacgt
     5641 gctggtgcgg cgcgacggcc cgcaggccgc gcgcgtcgat gcgttcttcg cctgggcgct
     5701 gggctcgggc gccgccatgg tcgacggcct cgattacctg ccgctgccgg ccgacgccgc
     5761 ggtgcgcgtg ctggcgacgc tgcggcacga ttgacgcgcg gaacgacgca cgcaaaagcc
     5821 ctggccgcgt cgtgcgcgca ggaacacgca agacctgcag ggcaggttgg ccgcaggcgc
     5881 aacccgccat gtccgcagat cgtcatgcag gcgaacggcg ggttacgcct tcggctaacc
     5941 cgccctacga aacccgcgcg tgggcgaagg tcctcacgcc gtctgcgtgc gctgcagctt
     6001 cagctcctcg atcatccggt cgcgcatcac gaacttctgc gccttgccgg tgaccgtcat
     6061 cggcaggtcg tcgacgaagc ggatgtagcg cggcaccttg tagtgcgcga tctggtcgcg
     6121 gcagaagtcg cggatctcgt cctcggtgga cgcctcgccg gccttgagca cgatccaggc
     6181 gcacacctcc tcgccgtact tggcgtcagg aacgccgaac acctgcaccg actgcacctt
     6241 cgggtggcgg aacaggaact cctcgatctc gcgcgggtag acgttctcgc cgccgcggat
     6301 cagcatgtcc ttcacgcgcc cgacgatgtt gcagtagcct tcctcgtcga tcgtggccag
     6361 gtcgcccgtg tgcatccagc cgtcgacgac cgcctcggcg gtgcgcttgt cgtcgcccca
     6421 gtagcccagc atgaccgagt agcccttggt gcacagctcg cccttctcgc cgaccggcac
     6481 gatgttgccg tcggcgtcga cgatcttgca ctccaggtgc ggctgcacgc ggccgacggt
     6541 ggtggtgcga cggtcgagcg ggtcggccgt cgagctctgg aacgacaccg gcgaggtctc
     6601 ggtcatgccg taggcgatgg tcacctcgcg catgttcatc ctcgagacga cctgcttcat
     6661 cgtctcgatc gggcacggcg agccggccat gatgccggtg cgcagcgagc gcaggtcgta
     6721 ctgcgcgaac gacgggtgcg ccagctccgc gatgaacatc gtgggcacgc cgtgcagcgc
     6781 ggtgcagcgc tcctcggcca cggccgacag cgtggccgcc gggtcgaacg cctcgccggg
     6841 gaacaccatc gccgcgccgg tggacacgca ggccagcact gccagcacca tgccgaagca
     6901 gtggtacagc ggcacgggga tgcacagcga gtccttgtcc gagaagttca tcgccatcgc
     6961 gatgaagcgg ccgttgttga cgacgttgtg gtgcgtcagc gtggcgccct tggggttgcc
     7021 cgtggtgccg ctggtgaact ggatgttgat cgcgtcgtgg cacaggcagt cggcgaaggg
     7081 caacggcgcg ccacgcccgc gttcgagcac gtcggcgtaa ttgagcatgc ccggcgagcg
     7141 ctctccgccc atgcggatga cgtgctcgag cgccggaagt tcgcgccgga ccttctgcaa
     7201 catctccagg tacatggacg tcttgagccg ctcggccgac acgatcgcgc ggcagcccga
     7261 gacctgcagc gcgtaggtca gctcggacag ccggtacgcc ggattgacgt tgaccagcac
     7321 caggcccatg cgcgcggtgg cgaactgcgt gacgagccac tcgacgcggt tgggcgacca
     7381 gatgcccacg cggtcgccct tggccagacc gagcgcggac aggcccgcgc cgaaggcgtc
     7441 gacctcggcg aggaactcgc gccaggtcca gcgcacgccc tgctcgcgga acacgacggc
     7501 caggcggtcc gggtagcact tggccgtgtc ggccagcagc ccgtagatcg tggcgtccga
     7561 cagcggcagg tcggtgcggc cgacgacgtg cgacttgccg tcgatcggaa gcgtcgggac
     7621 agttgctgcg ttcatcgcgg tctccatctc gttgtgccgg cggcgccgag gccgctttcg
     7681 cactggagat taaaccagct tcgtgaaccg gaacttacat gggcgggccc gtgcgtctgc
     7741 gatggttcca ggagtctgcg cggcagagaa cgccgcctgc aattccgcca gctcgtcgag
     7801 ggcgacggcg ccctctgccg cgcagccctt agaaccaatg catgtagcgc agctggaacc
     7861 agttctcccc ggggttgggg tgcttgatcc cgccgttcga gtagtgctcg gcgcgcagct
     7921 cgatctcgtc gcgcccctcg tcgccgaact tgcgcccgag gccgacgtgg tcgccgaagt
     7981 tgaaatcgct gccgaagcgc cgcctgtccg tcttccaggc cggcgcgatc acgtcgacgc
     8041 cgatgcccag ttccatgtac cagtgctccc accacgaggg ctcgtagcgg accaccggcg
     8101 tgaggccgat ctgcgtggag cacgcgtggt actgctcgcc ggcctcgcga tggcaggccc
     8161 attcgccgat ggatgtctcg agatagccgc tcgcattgcc ggcgccccac ttcgagggct
     8221 gtacccatgt ccatgccacg ccgcccacga cggagcgcga atcgtccgag attccgccct
     8281 cgatgaacac gccgtacggc gacgtcagct ccttggcgcc ggcggccgcg ggcgcgagcg
     8341 ccgccgcggc cacggccagg cccatcagcg cgggtggcgc cgccttgcgt ctctcgttgc
     8401 ccaactcgca ctcccctgtc gtgttgaccg cgcgctcctg cgcggcgtcg tggccagggc
     8461 cgtcggcaag gcgcgttccc gcgcggcagt ggtccgtcag tccggcagct tgaagaggcg
     8521 ccggctgttg gcgctggcct gcagggacag cgccgccacg tccatgccct tcaactcggc
     8581 caccgcccgg gcgacgaacg gcagcagcgc cggctcgttg tacggccgcg tcttgcccgc
     8641 gttcttcgga cgcaggtacg gcgcgtcggt ctccaggtgc aggcgcccgg cggggacggc
     8701 gcgcaccgcg tcgcggagcg cgctgccgcg ggtctcgtcg gtgacccagc cggtgatgcc
     8761 gatgtcgaag ccgagcgcga ggaaggcctc ggcctgcgcc gggccctcgg tgaagcagtg
     8821 gacgacacca tgtgcgccgg cggcggcggc atcgcgcagc atggcgtgga agtcgtcgaa
     8881 ggcgtcgcgg cagtgcagga acagcggctt gtccagccgc acggcggcgg cgatctgcgc
     8941 ctcgaatgcg gcgcgctggt cctgcatcgg cgagaacatg cggttgtagt cgaggccgca
     9001 ttcgcccacc gcgacgacgc gctcgtcggc ccacagcggc tcgaactgcg cgggcgagtg
     9061 cgacgtccag tgcctggcgt catgcgggtg cacgcccgcg gtcgcccaca ccctcgcatg
     9121 cgtgcgcgcg aacgcgagcg cgagctcgct ggccagcagc gaggtgccgg tggcgaggat
     9181 gtgcgtgacg cccgcggccg cggcgcggga cagcagctcg gcatccttgc ccttgagttg
     9241 cgaggagtgg aagttgacgc cgatgtcgat gaggctcatg ggcgcattgt ctccgcgcga
     9301 tggacgagcg ggcgcggcga acgcctcacc gcaggaactt cggcagctcg ctcttgaact
     9361 tgctcagcgc cagcggcttc gtccagtacg cctccgcccc ccgcgcgatg gcctcggtca
     9421 cctgcgccgg catcgcgtcg gccgacagcg ccaccacgcg cagcccgctg gtgaccgggt
     9481 cggcgcgcaa ccggtccagc acgtccagcc cgctcatgtc gggcagttgc atgtccagca
     9541 ggatgaggtt gggcatccag cgccgcgcca gctccagccc ggactccccg tcacgggcga
     9601 ccagcagctg cacgtcggag gtcagcgcca cgtactgctc cagcagcagg atgttgaccg
     9661 ggtcgtcctc gatgtagacg atcgtgccgg ccgcccccgc cgtgccctgg ccgaacgcgg
     9721 tggtggcgac gaagtcggtg tgctcgacgt cctcgtccag gtcgcggttc aggcggatcg
     9781 tcaccgtcgt gcccttgccg ggcgcgctgg cgatgtccag cgagccgccc tggcgctcgg
     9841 ccaggtggcg gctgatgacc aggcccaggc cggcgccctc cgtggcgttg ggctcgtcgc
     9901 cgagccggcg gaacgggatg aacaggtcac gctgctgctg cgcgctcatg ccggcgccct
     9961 cgtcgcgcac ggtcagcacg gcctgcgtgg gcgtgagcga gaagccgatg tagacgcgtc
    10021 cgccgcgccg gttgtacttg atcgcgttgg acaggatgtt gacgaacacc tgcttgagcc
    10081 gcaccgggtc ggccacgacg aacagctgcg acggcggttc gtccaggtcg atcgacacgt
    10141 cgcgccccgc gcgcgccgtc tccagcaggt cgacggcgtt gcgcagcacc ggcgcgaggc
    10201 gcacgctgct cagtgcgacg ctcagctcgc cgacctccag ccgcgcgacg tccagcaggt
    10261 cgttgaccag ccgcagcagg tactgcccgc cgtcgacgac gcgatcgagc cgcccgcgct
    10321 gcgtggccgt gagcggctcg tcgcggtcgg tgcgcatgag gtcggagaag ccgatcaccg
    10381 cattgagcgg cgtgcgcagc tcgtggctga cccgcgacag gaactcgcgc ttggcctggt
    10441 aggtgcgctg cgcggcgccc tcctccagca tcgcgttctg cgcgttgacc tcgtcggtga
    10501 cgtcgcgcac caggcccacc atgtgcgaga cggtgcggcc gtcggccatg aacgcgcggc
    10561 cgtcgatctc cagcgtgcgc atgcggcccg acggcagcac gacgcgaaag cgctgcacga
    10621 ccggcgtgcg cgccgcgatc gcgttgagcg actcctgctt catgcgcgga cggtcgtccg
    10681 gatgcacgcg cagccgcagg tcgcgcgcct tgtagacgtt gcccggcgcg ggcggcaact
    10741 cgtagatggc gatggcgtgc gggtcgagca ggatcgtgtc gtcgcgcacg cgccattcca
    10801 tcaggcccag ccggccggca tcggcggcga gcgaccagcg cgcgttgccg tcctcgacct
    10861 cctgctgcga ctgcgcgagc tgcttccaca tctggcgcgc gaacatcagg ccgccggcga
    10921 tcagcgccag cgccagtccc gcggtggcca gcagcagcag gccctgcacc cagcgcgcgg
    10981 cctgccccag ctggcgcgag aacagcagct cgcgctggtt gagcgcgtcg tcgagccggt
    11041 acatctcggc ctcgaagcgc ggacgccagg cgtcgtcgtc gccggcgttg cgtgcctggt
    11101 ggatctgctc gcccaggcgc tgcagccgca cgatgtcctc gtcggcggcg gcccaggtgt
    11161 cgaccgccgc ggccaccagc ggcacgtggc ggaacaggcg gtaga
//
LOCUS       DATCRO010000503         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_793289, whole genome shotgun sequence.
ACCESSION   DATCRO010000503 DATCRO010000000
VERSION     DATCRO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793289"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIN75_13765"
     CDS             complement(<1..210)
                     /locus_tag="VIN75_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975285.1"
                     /translation="MIPIPATMSDDQTLTPSTMAKGDVARMDRAEQALAAMADVAGAR
                     VLRRADLTVAFVAAADAQVADAALLA"
     gene            528..1286
                     /locus_tag="VIN75_13770"
     CDS             528..1286
                     /locus_tag="VIN75_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8975286.1"
                     /translation="MPITTPPERVLPARAIAAPAGELERWARPLAAAAGPWPLCVVRH
                     DPALFRPDAFDEHGLLRPPAIARSVLRRQAEFFHGRMAARRALGALGHGAASALVGAA
                     GQPVWPAGVVGSITHSGPYAAAVALDGAQWRGVGIDIECVADQSGCAALTSVAIDGAE
                     LATLRAQAGRRDIAELLTLAFSAKESLYKAAFDAVGRYFGFECARVLEVDVAGARLAL
                     RIEEDLGGPFMRGHVCQVCIDTLAEGYVLTSFAW"
     gene            complement(1259..2539)
                     /locus_tag="VIN75_13775"
     CDS             complement(1259..2539)
                     /locus_tag="VIN75_13775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8975287.1"
                     /translation="MTDELAILGGKPARATPFPQWPVHGQPEEAALLAALASGRWGRL
                     DGDQVATFERRFADWLGVRHAIAVANGSVSLRIALLAAGIQAGDEVVLPAYTFVTTAG
                     AVVECNATPVFADVARDTFTLDPAAVERLVTPRTRAVIAVHLGGAPCDMDALMAIARE
                     RGLVVIEDAAHAHGTTLHGRHAGALGHLGSFSFQSSKNLTCGEGGLVTTDDDELARAC
                     RSIQNCGRVEEGAWYEHHVIGGNYRLGEFAGAILNAQLDRLDEQVQRRDANARRLDQA
                     VSSIRGLSAQALPSGTTRRAGHLYPLRYDEAAFGLPRARFLQALRAEGIPASAGYVQP
                     LYRQPLFARRAFGPYTGCAGRYDDVLLPHTEALCRGEALWIPHAALLGTARDTDDVAH
                     ALRKIGDRREALVAAFERLDARQRAAHHANDVST"
     gene            2677..>3793
                     /locus_tag="VIN75_13780"
     CDS             2677..>3793
                     /locus_tag="VIN75_13780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8975288.1"
                     /translation="MIPAASKSRNERIDVLRGVAIACVLVLHFTLAYGLKDSPVGSVL
                     PRWLLAGLTLNGNYGVTMFFAISGFLITSLSLRRWGSLAKIDLRSFYVYRAARILPPL
                     VPVLAVIVALGCAGLPHFSNSDDGHDLPASFFAAGAGSVLTFWHNVLMQQYGYFNYCL
                     NIYWSLSVEEVFYVLMPLLCLGLRRNGAMLVACLGFIALGPWYRARHLDDEIFYLYAY
                     QACFDAIAFGCIAAFAAAARPLAGRWATACRWSASLALVAVYLSGIQHHVVWGFTGMA
                     AGTAVFLYAAAHAAPPGPRALALTAPLRWLGRHTYELYLVHIVVLAGLQDVLGRADMT
                     HGLRLPWMALFLLLSCAVAAATARWVAQPVEAALRRRF"
BASE COUNT          476 a         1341 c         1358 g          618 t
ORIGIN      
        1 cgccagcaag gccgcgtcgg ccacctgcgc gtccgcggca gccacgaacg ccaccgtcag
       61 gtccgcccgc cgcagcaccc gggcgccggc gacgtccgcc atcgcagcca gcgcctgctc
      121 ggccctgtcc atcctcgcga cgtcgccttt ggccatcgtg ctcggcgtga gcgtctggtc
      181 atcggacatc gtggctggaa tggggatcac ggctaacctc tgattgaagc ggcggcaacg
      241 cttcgtgggc gtggtcgacc tctggggcgg cggatgatag caacgaagat gtgccggtta
      301 cgtgacgttc cggtgatctc ttctaggggt tttccgatgc ataccctggt tacggtatgg
      361 ggtatgaagt caatcccttg tatatacggc ctgccgtatt cagccagagg tcagtagccg
      421 acgttacgca tcgatacctg acgggtcgga acggacgcag gaaggaccct cggcgcacgt
      481 aagatcgcgc gctccctgcc gaccgacccc acttcgaccg cgacgacatg cccatcacca
      541 cgccgcctga gcgcgtcttg cccgcgcggg cgatcgcggc acccgcagga gagctggagc
      601 gctgggcgcg gccgctggcc gcagcggccg ggccctggcc gctgtgcgtc gtccggcacg
      661 atcctgcgct gttcaggccg gacgcattcg acgagcacgg cttgctccgg ccgcccgcca
      721 tcgcgcgcag cgtgctgcgg cgccaggccg agttcttcca tggacggatg gccgcgcggc
      781 gtgccctggg cgccctcggc cacggcgccg cgtccgcgct ggtcggcgcc gcgggccagc
      841 ccgtctggcc tgccggcgtg gtgggcagca tcacgcacag cgggccctat gctgcggcgg
      901 tcgcactcga cggtgcgcag tggcgtggcg tggggattga catcgaatgc gtggcggacc
      961 agtcgggttg cgcggcgctc acgagcgtcg cgatcgacgg cgcggagctg gccacgctgc
     1021 gcgcgcaggc gggccgccgg gacatcgccg agctgctgac gctcgcgttc tcggccaagg
     1081 aaagcctgta caaggccgcg ttcgatgcgg tcggccgcta cttcggcttc gagtgcgcgc
     1141 gggtgctgga ggtcgatgtc gccggcgccc ggctcgcgct gcgcatcgag gaggacctgg
     1201 gcggtccgtt catgcgcgga catgtctgcc aggtatgcat cgacaccctg gccgagggct
     1261 acgtgctcac gtcgttcgcg tggtgagccg cgcgctggcg cgcatcgagc cgctcgaacg
     1321 ccgccaccag cgcctcgcgc cgatccccga tcttgcgcag ggcgtgcgcc acgtcgtcgg
     1381 tgtcgcgcgc cgtcccgagc agggccgcgt gcggaatcca gagggcttcg ccgcggcaca
     1441 gcgcctcggt gtgcggcagg agcacgtcgt cgtagcgccc cgcgcaaccg gtgtacggac
     1501 cgaaggcgcg tcgcgcgaac agcggctgcc ggtacagcgg ctgcacgtag ccggccgagg
     1561 cggggatgcc ttcggcgcgc agcgcctgca ggaagcgcgc gcgaggcagg ccgaaggccg
     1621 cctcgtcgta gcgcagcggg tacagatgcc cggcgcgtcg cgtcgtgccg gacggcaggg
     1681 cctgcgcgct caggccgcgg atggaggaca cggcctggtc caggcgacgc gcgttcgcgt
     1741 cgcggcgctg cacctgctcg tcgagtcgat cgagctgcgc gttgaggatc gcgcccgcga
     1801 actcgcccag ccggtagttg ccgccgatga cgtggtgctc gtaccaggcg ccttcctcga
     1861 cgcgtccgca gttctggatc gagcggcacg cgcgcgcgag ctcgtcgtcg tcggtcgtga
     1921 cgaggccgcc ctcgccgcag gtgaggttct tgctcgactg gaacgagaac gagccgaggt
     1981 ggcccagcgc gcccgcgtgg cggccgtgca gcgtcgtgcc gtgcgcgtgc gcggcgtcct
     2041 cgatgaccac caggccgcgc tcgcgggcga tggccatcag cgcgtccatg tcgcacggcg
     2101 cgccgcccag gtgcaccgcg atgacggcgc gcgtgcgcgg cgtcaccagg cgttccacgg
     2161 cggccggatc gagggtgaac gtgtcgcgcg ccacgtcggc gaacaccggc gtcgcgttgc
     2221 actcgacgac ggcgccggcg gtcgtgacga aggtgtacgc gggcagcacg acttcgtcgc
     2281 cggcctggat gccggcggcc agcagcgcga tgcgcaacga cacgctgccg ttggccaccg
     2341 cgatggcatg gcgcacgccc agccagtcgg cgaaacggcg ttcgaaggtg gcgacctggt
     2401 cgccgtcgag gcgtccccag cggccgctgg ccagggcggc cagcagcgcc gcttcttcgg
     2461 gctggccatg gacgggccac tgcgggaagg gcgtcgcacg cgcgggcttt ccgcccagga
     2521 tcgccagttc gtcggtcatc gattcgctcg ggatgtcgga gacgccattc tcgacgctgc
     2581 cgctcgccag gcgctcatac ggtcgtgggt atcatcacgg gccttccgat gcgcgtgccc
     2641 ggcgtcgcag gaactgtcca ccatcgatcc cttgccatga ttcctgccgc atcgaaatcc
     2701 cgcaacgagc gcatcgacgt cctgcgaggc gtcgccatcg cgtgcgtgct cgtcctgcac
     2761 ttcacgctgg cgtacggact caaggacagc cccgtcggca gcgtcctgcc ccgatggctg
     2821 ctggccggcc tcacgctcaa cggcaactac ggcgtgacca tgttcttcgc gatctccgga
     2881 ttcctcatca cgtcgctctc gctgcgccgc tggggcagcc tcgcgaagat cgatctgcgc
     2941 tcgttctacg tgtaccgcgc cgcgcgcatc ctgccgccgc tggtgccggt gctcgcggtg
     3001 atcgtcgcgc tcggctgcgc ggggctgccg cacttctcga actcggacga tggccacgac
     3061 ctcccggcgt cgttcttcgc ggccggcgcc ggctcggtgc tgaccttctg gcacaacgtg
     3121 ctgatgcagc agtacggcta cttcaactac tgcctcaaca tctactggtc gctgtccgtc
     3181 gaggaggtct tctacgtgct gatgccgctg ctgtgcctgg gcctgcggcg caacggcgcc
     3241 atgctcgtgg cctgcctggg attcatcgcg ctggggccgt ggtaccgcgc tcgccatctg
     3301 gatgacgaga tcttctacct gtacgcctac caggcctgct tcgacgcgat cgcgttcggc
     3361 tgcatcgcgg cgttcgcggc cgcggcgcgc ccgctggccg gtcgatgggc caccgcgtgc
     3421 cgctggtcgg cgtcgctggc gctggtcgcc gtgtacctgt cgggcatcca gcatcacgtg
     3481 gtctggggct tcaccggcat ggccgcgggc acggcggtgt tcctgtatgc ggcggcccac
     3541 gcggcgccgc ccggaccgcg cgcgctggcc ttgacggcgc cgctgcgctg gctgggccgg
     3601 cacacctacg agctgtacct cgtgcacatc gtcgtcctgg cgggactgca ggacgtgctc
     3661 gggcgcgccg acatgacgca tggcctgcgc ctgccctgga tggcgctgtt cctgctgttg
     3721 tcgtgcgcgg tcgccgcggc gaccgcgcgc tgggtggcgc agccggtcga ggccgcgctg
     3781 cggcggcgct tcc
//
LOCUS       DATCRO010000504         8654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_422248, whole genome shotgun sequence.
ACCESSION   DATCRO010000504 DATCRO010000000
VERSION     DATCRO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422248"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VIN75_13785"
     CDS             <1..687
                     /locus_tag="VIN75_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HEY8975289.1"
                     /translation="RAPNVVEVVGEPYFNKSAAQNIGAFSTRQRVLFFCDCDIILDPV
                     QVAALAARVDQSPGVFATLAGVRESEENSRGGNHVVSFGYQLDIRTADGRRLRIVDNE
                     EDIESGTRQAPGLLLVRRQDFLSIDGYNGTLHGWGWEDQDMISRLTLGAGLERLIEGT
                     AIHLSHGDAARTAFYPEVASRWESRDRMFRTALSNYDAANFRGTYSADVARLGGAVKS
                     QREAPAWQPA"
     gene            672..1643
                     /locus_tag="VIN75_13790"
     CDS             672..1643
                     /locus_tag="VIN75_13790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8975290.1"
                     /translation="MAARLSVCMIVRDESAVLDRCLASLAGLVDELCIVDTGSVDDTP
                     SIARRHGARMASFTACNDDQGRIVDFALARNAALAMATGEWVLQIDADEVLEAGADRV
                     RHWLRSGEADCVAVRMKSGGAEWVSQRLFRRAQASTYRSRIHEYVECGGIAARDVGIR
                     IVNLQDKRGKESSSDRNRRLCELALAENPDDARILFYLAGENRKAGKLQEAVEQFTRC
                     IALGTFRQGLYRAMFQLGACHLMLGRWAAAMDAARSAIALDGRYAEGYCLLADACACS
                     ERLDEARRHYRTALACTPPADALFPVEGWAYDAHPRRRLAAMGALPA"
     gene            complement(1681..2184)
                     /locus_tag="VIN75_13795"
     CDS             complement(1681..2184)
                     /locus_tag="VIN75_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEY8975291.1"
                     /translation="MKHPRIQVASQALAALLLCSAGASAAPARQAAASTPVGVTAAFY
                     DWYITKFRSDHDPMFELRSGAQAWVSSSLLDEVDARLEDQVTDDDEFLQVDDMVRPCH
                     SLAAEPLGEAHGRAEVGVTLGARTTHPWRLRVALVKEDGVWRIRHVERDRARPSPTAA
                     RRAMSDC"
     gene            complement(2297..3376)
                     /locus_tag="VIN75_13800"
     CDS             complement(2297..3376)
                     /locus_tag="VIN75_13800"
                     /inference="COORDINATES: protein motif:HMM:NF044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975292.1"
                     /translation="MSAQADRPATPDVVVITSSRDPDARFVARAVRRHGGTVFSWHTD
                     EISADQSLGLEVGADRASAVVRTPQGTLDLLDPSHGVRSVWLRRMALPRLPAAIHPDD
                     AAFAEAENQALLRAIFQCAFPGALWVNGWAAGRRAEAKLVQLLEARRVGLTLPPTLIS
                     NDRADIESFMARRAEGQTLYKPFNFKEWTLGDGQRRTAFANRVTCANLPSAPLQRANP
                     GIFQQFVDKRFDARLVLFGSTCIAAAIHGNGSVDWRHASRMGRSRVEPLDAPSTVVHK
                     CLQMMRSLAIAYASFDFVVDHDGQYHFLELNEAGQFLWLEEVCPDLPLADGFARFLIH
                     GDLGFDAAAGASTDRVTLDSVLARA"
     gene            complement(3392..3964)
                     /locus_tag="VIN75_13805"
     CDS             complement(3392..3964)
                     /locus_tag="VIN75_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975293.1"
                     /translation="MRHARLPVAALALAASLVPAASRAATDCAAEYARHLESDLALSY
                     REFDQSESSGYRALAAQGCRAQAAALLERYIARSHDLERVMWWHSAQLKAEAGDYAAA
                     IATARKSLAASEDFDRNPIRWNDYVQATIAFWQHDRDALRQHRAVVAAGAADFLPNQI
                     NLDMLDSLTANFDLGYADAMARVRHASRQD"
     gene            complement(4071..4256)
                     /locus_tag="VIN75_13810"
     CDS             complement(4071..4256)
                     /locus_tag="VIN75_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975294.1"
                     /translation="MNTDIDTIRPEAEERLLGFQVGVTIPEEALGLIAGAGGQAGCVL
                     TDHGNDAFCTNPGMGGQ"
     gene            complement(4300..4485)
                     /locus_tag="VIN75_13815"
     CDS             complement(4300..4485)
                     /locus_tag="VIN75_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975295.1"
                     /translation="MTVVSQAPASTIDAEAERLTGFQVGVTIPDEALDLVSGARMSPS
                     NCVLTDHGEDAFCAAMR"
     gene            complement(4615..6774)
                     /locus_tag="VIN75_13820"
     CDS             complement(4615..6774)
                     /locus_tag="VIN75_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEY8975296.1"
                     /translation="MRKLIQSGFFARRALPVFLQAEASECGLACVAMVGAFHGHEIDM
                     TSARRRFPISSKGVTFRTLIDMARRLSLQARPVKVELDGLAQIRTPCILHWEMKHFVV
                     LKRATPRHVVIVDPAVGERRLAWPVVSRSFTGVALELWPENSFQAIDDRPSYTLRSLA
                     GKVTGLKSGLLKLFCLGVLLQVCALVVPFNAQWVIDYALVDDDTSLVNTLAVGFVLLM
                     LFQAFILFLKSWITAVITTDFTFQWLGRTFGHLLRLPMDYFERRHLGDVASRFASIQL
                     IQQSITVQLVDGVLDALLVLGAGVLLFVYSPALGAISVAATLAYAAARGAFVSPQRAA
                     TTEQIVSAAQQQAYLLETVRCMQGIKLFNRTETRRVGWMNKLAEQCNAQLRIARISTA
                     SQSISALIFGIERVVVVWAAAHLVMDTRFSVGMLFAYLSYREQFSARVASLIDKVYDI
                     QLLKLHGERVADIVMSRPEADVLSDGETDGSVAASSAIDVHGLDFRYSDSDPWMFNGL
                     SLNFADGECVALTGSSGCGKTTFVKLLLGLLEADGGYVSVAGVPIRKMGIGNFREMVG
                     TVMQEDQLLTGTIAENICFFDPSPDFERIEECALMSAIDEEISRMPMGYNTLVGDVGA
                     GLSGGQKQRILLARALYRKPRILVLDEATSNLDVPNERTVMSAVKKLRMSRIVVAHRP
                     ETIAMADRVLRFEPGKGSFGAVAVVEAATPRAATAAA"
     gene            complement(6779..8032)
                     /locus_tag="VIN75_13825"
     CDS             complement(6779..8032)
                     /locus_tag="VIN75_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8975297.1"
                     /translation="MFREEAQQHAQNGSIGSLVLDSQFTLTLVAYASAVIFSVAMYFV
                     LTADVARKTQISGAVVPVNGAVGVRSLLDGTVVGVQVGEGQRVKAGQVLFEVSTQRGD
                     RSNAAREGRIEGLLTSRKQSLAEQARLQSQQDANDMLALAHRISSLDVEIDRLGQQVQ
                     LQQERIKLAESDLSRNTQLEKAGFMSGAKTRALTADLLDQKLKGEDLAHQQASARIDR
                     DDVQSRLAALRNKAQQDARSAERDTTEIDQAMIEREENKLVQVVAARDGVLSGVTVEV
                     GQPVIANQVLATLLPADGVLGVTLYAPPDAIGNVKVGMPVKIRYHAFPYQKYGQFPGV
                     VQEISQVALRQEDLQFFESGATRGRDFPYKFRIRVALKGQTVRVGNADEALKPGTTVD
                     ATVMGETRKVYQLIFPVETFLTSGT"
BASE COUNT         1301 a         2906 c         3103 g         1344 t
ORIGIN      
        1 cgggcgccga acgtcgtgga ggtggtgggc gagccgtact tcaacaagtc ggcggcgcag
       61 aacatcggcg cgttcagcac acgccagcgc gtgctgttct tctgcgactg cgacatcatc
      121 ctcgaccccg tgcaggtggc cgcgcttgcg gcacgggtcg accagtcgcc gggcgtcttc
      181 gccaccctgg cgggcgtgcg ggagtccgag gagaactcgc ggggcggcaa ccatgtcgtg
      241 tcgttcggct accagctcga catccgcacg gccgacgggc gccggctgcg catcgtcgac
      301 aacgaggaag acatcgaatc gggcacgcgc caggcgcccg gcctgctcct cgtccgccgc
      361 caggacttcc tgtccataga cggctacaac ggcaccctgc acggctgggg ctgggaagac
      421 caggacatga tctcgcgcct cacgctgggc gccggcctgg aacgcctcat cgagggcacg
      481 gcgatccacc tgtcgcacgg ggacgccgcc cgcaccgcgt tctatcccga agtggcgtcg
      541 cgttgggaaa gccgcgaccg catgttccgc acggcgctgt cgaactacga tgccgccaac
      601 tttcgaggca cctacagcgc ggacgtcgcg cgcctgggcg gagcggtcaa gtcccagcgg
      661 gaggcgccgg cgtggcagcc cgcctgagcg tctgcatgat cgtgcgcgac gagtcggccg
      721 tgctggatcg ctgcctcgcg tcgctggcgg gcctggtcga cgagctgtgc atcgtcgata
      781 ccggctccgt cgacgacacg ccgtcgatcg cccggcgcca cggcgcgcgg atggcgtcgt
      841 tcacggcgtg caacgacgac caggggcgca tcgtcgactt cgcgctcgcc cgcaacgccg
      901 cgctggccat ggccaccggc gagtgggtgc tgcagatcga cgccgacgag gtgctcgagg
      961 cgggcgccga ccgcgtccgc cactggctcc ggtccggcga ggccgactgc gtcgcggtgc
     1021 ggatgaagtc gggcggcgcg gagtgggtgt cgcagcggct gttcaggcgc gcccaggcct
     1081 cgacctaccg cagccgcatc cacgaatatg tcgagtgcgg cggcatcgcg gcgcgcgacg
     1141 tgggcatccg gatcgtgaac ctccaggaca agcgcggcaa ggaatcgtcc agcgatcgca
     1201 accgtcgcct gtgcgaactg gcgctggccg agaacccgga cgacgcccgc atcctgttct
     1261 acctggccgg cgagaaccgc aaggccggca agctccagga ggccgtggag caattcaccc
     1321 gctgcatcgc gctcgggacg ttccggcaag ggctctatcg cgccatgttc cagctgggcg
     1381 cctgccacct gatgctcgga cggtgggccg cggcgatgga cgccgcacga tcggcgatcg
     1441 cgctcgacgg ccgatacgcg gaggggtatt gccttctcgc cgacgcctgc gcatgcagcg
     1501 aacgcctgga cgaagcgcgc cgccactacc gcacggcgct ggcgtgcacc ccgccggccg
     1561 atgcgctgtt tcccgtcgag ggctgggcct acgacgcgca tccgcgccgg cggctggcgg
     1621 cgatgggcgc cctgccggcg tgaggcctgc gggccgccgt gcccgcaggc tggccgcgtt
     1681 tcagcagtcc gacatcgcgc gacgggccgc ggtgggcgac ggccgcgccc ggtccctttc
     1741 cacgtgccgg atgcgccaga cgccgtcttc cttgacgagc gccacgcgca agcgccacgg
     1801 gtgcgtcgtg cgcgcgccca gcgtcacgcc gacctcggcg cggccatgcg cttcgcccag
     1861 cggctcggcg gccaggctgt ggcacgggcg gaccatgtca tcgacctgaa ggaactcgtc
     1921 gtcgtccgtg acctggtcct cgagccgggc gtcgacttca tcgagcaggc tcgacgacac
     1981 ccaggcctgc gcgccggatc gcagctcgaa catcggatcg tggtcgctgc ggaacttggt
     2041 gatgtaccag tcgtagaagg cggcggtgac gcccaccggc gtggacgccg ccgcctggcg
     2101 ggccggcgcc gcggaggccc ctgcactgca cagcagcagg gccgcgaggg cctgcgacgc
     2161 gacctggatc cgtgggtgct tcatggcgtc gttcgtcgcg agcggccgat cggaggtgtc
     2221 atggggcggc agtatcgctg caagcgcatg cgtgcgacgc ggtacccttg ctggcacgca
     2281 ggttgcgcgg cgaagctcag gcccgggcca gcacgctgtc cagggtgacg cgatcggtgg
     2341 acgcgcccgc ggccgcgtcg aaaccgaggt cgccgtggat caggaagcgc gcgaacccgt
     2401 cggccagcgg caggtccggg cagacctcct cgagccacag gaactggccg gcttcgttca
     2461 gctcgaggaa gtggtactgc ccgtcgtggt cgacgacgaa gtcgaacgac gcataggcga
     2521 tggccagcga gcgcatcatc tgcaggcact tgtgcaccac ggtcgagggc gcgtccagcg
     2581 gctcgacgcg gctgcgtccc atccggctgg cgtggcgcca gtcgacgctg ccgtttccat
     2641 ggatggccgc ggcgatgcag gtgctgccga acagcaccag tcgcgcgtcg aagcgcttgt
     2701 cgacgaactg ctggaagatg cccggattgg cgcgctgcag gggcgccgac ggcaggttgg
     2761 cgcaagtcac gcggttggcg aaggcggtcc gccgctggcc gtcgcccagc gtccattcct
     2821 tgaagttgaa cggcttgtag agcgtctggc cttcggcgcg tcgcgccatg aaggactcga
     2881 tgtcggcgcg gtcgttggag atcagcgtcg gcggcagcgt cagtccgacg cggcgtgcct
     2941 cgagcagttg cacgagcttg gcttcggcgc gccgccccgc ggcccagccg ttgacccaca
     3001 gggcgccagg gaacgcgcac tggaagatgg ctcgcagcag cgcctggttc tcggcctcgg
     3061 cgaacgcggc gtcgtccgga tggatggcgg cgggcaggcg cggcagggcc atgcgccgca
     3121 accagaccga gcgcacgccg tgcgagggat cgagcaggtc gagcgtgccc tgcggcgtgc
     3181 gcaccacggc cgaggcgcgg tcggcgccca cctccaggcc gagcgactgg tcggccgaga
     3241 tctcgtcggt gtgccaggaa aagaccgtgc cgccgtgccg ccgcaccgcg cgcgcgacga
     3301 accgggcgtc gggatcgcgc gacgaggtga tcacgacgac gtcgggcgtg gcggggcggt
     3361 cggcttgcgc ggacatggcg gcggctcgtg ctcagtcctg gcgcgaagcg tgcctgacgc
     3421 gcgccatcgc gtcggcatac ccgaggtcga agttggccgt caggctgtcg agcatgtcca
     3481 ggttgatctg gttggggagg aagtcggccg cgccggccgc gacgacggcg cggtgctggc
     3541 gcagcgcgtc gcgatcgtgc tgccagaagg cgatggtcgc ctgcacgtag tcgttccagc
     3601 ggatcgggtt gcggtcgaag tcctcgctcg cggccaggga cttgcgggcc gtcgcgatcg
     3661 ccgccgcata gtcgccggcc tcggccttca actgggcgct gtgccaccac atcactcgtt
     3721 ccaagtcgtg cgagcgggcg atgtagcgct cgagcagggc ggcggcctgc gcgcggcagc
     3781 cttgcgcggc gagggcgcga tagccggagg attcggactg gtcgaactcg cgatacgaga
     3841 gtgccaggtc ggactcgagg tgcctggcgt attcggccgc gcagtccgtc gcggcgcggg
     3901 aggcggcggg gacgagcgat gcggccaggg caagcgcggc gacgggaagg cgggcgtggc
     3961 gcatggtggg ttcttcacgc ggatcggcgt gacggggtgg aagcgggcga gcaaaaggcg
     4021 gagagtcgcc atgcgctctc cgccccggca tcaggcgatg cgttgtcggc ttattggccg
     4081 cccatgcccg ggttggtgca gaaggcgtcg ttgccgtggt cggtcagcac gcagccggcc
     4141 tggccgcccg cgcccgcgat caggccgagg gcttcctcgg ggatcgtgac gccgacctgg
     4201 aaacccagga gacgttcttc ggcctcgggg cggatggtat cgatgtcggt gttcatgtgg
     4261 ttccctgtag atttgttgat tggatcggat gccgcgcgtt cagcgcatcg cggcgcagaa
     4321 cgcgtcttcg ccgtgatcgg tcagcacgca gttggacggc gacatgcgcg cgccgctcac
     4381 gaggtcgagc gcctcgtcgg gaatggtgac gcccacctgg aagccggtca ggcgttcggc
     4441 ttcggcatcg atggtcgagg ccggggcctg ggacacaacg gtcattgctc ttctcctttg
     4501 gattgaatcc tcgtcgagga tattccggac gatccggaat tcgggagacg gcatcgcgcg
     4561 tcgcgctgcc gccgtgtctt gtcgcgtcgc cgccggccga aggcatcgca tcgctcaggc
     4621 cgccgcggtt gccgcgcgag gggtagccgc ttccacgacc gcgacggcgc cgaacgagcc
     4681 cttgcccggt tcgaagcgca ggacgcgatc ggccatcgcg atcgtctcgg ggcggtgggc
     4741 gacgacgatg cggctcatgc gcagcttctt gaccgcgctc atgacggtgc gttcgttcgg
     4801 gacgtccagg ttgctggtcg cctcgtccag caccaggatg cgcggcttgc ggtagagcgc
     4861 gcgcgccagc aggatgcgct gcttctggcc gccggacagg ccggcgccga cgtcgcccac
     4921 cagggtgttg tagcccatcg gcatcctgga gatttcctcg tcgatggcgc tcatcagggc
     4981 gcactcctcg atccgctcga agtcgggcga cgggtcgaag aagcagatgt tctcggcgat
     5041 ggtgcccgtg agcagctggt cttcctgcat cacggtgccg accatctcgc ggaagttgcc
     5101 gatgcccatc ttgcggatcg gcacgccggc gacgctcacg tagccgccgt ccgcttcgag
     5161 caggcccagc agcagcttga cgaaggtcgt cttgccgcag cccgagctgc cggtcagcgc
     5221 gacgcactcg ccgtccgcga agttcagcga caggccgttg aacatccacg ggtcgctgtc
     5281 ggagtagcgg aaatccaggc cgtgcacgtc gatggcggag gacgcagcca cgctgccgtc
     5341 cgtctcgccg tcgctgagca cgtcggcctc cgggcgtgac atgacgatgt cggcgacccg
     5401 ctcgccgtgc agcttgagca gctggatgtc gtagaccttg tcgatcagcg acgcgacgcg
     5461 cgcggagaac tgctcgcggt agctcaggta ggcgaacagc atgccgaccg agaagcgcgt
     5521 gtccatcacg aggtgcgccg cggcccagac cacgacgacg cgctcgatgc cgaagatgag
     5581 ggcgctgatc gactggctgg cggtcgagat gcgggcgatg cgcagttgcg cgttgcactg
     5641 ctcggccagc ttgttcatcc agccgacgcg gcgcgtctcg gtccggttga acagcttgat
     5701 gccctgcatg cagcggacgg tctcgagcag gtacgcctgc tgctgcgcag ccgagacgat
     5761 ctgctcggtc gtcgcggcgc gttgcgggct cacgaacgcg ccgcgcgcgg ccgcgtacgc
     5821 cagcgtcgcc gccaccgaga tcgcgccgag cgccgggctg tagacgaaca gcagcacccc
     5881 ggcgcccagc acgagcaggg cgtcgagcac gccgtcgacg agctgcaccg tgatgctttg
     5941 ctggatcagc tggatggagg cgaagcgcga ggcgacgtcg ccgagatggc gccgctcgaa
     6001 gtagtccatc ggcagccgca gcaggtggcc gaaggtgcgc cccagccact ggaacgtgaa
     6061 gtcggtcgtg atgaccgcgg tgatccacga cttcaggaac aggatgaacg cctggaacag
     6121 catcagcagc acgaagccga ccgccagcgt gttgacgagg ctggtgtcgt cgtcgaccag
     6181 cgcgtagtcg atcacccact gggcgttgaa aggcacgacc agcgcgcaca cctgcagcag
     6241 cacgcccagg cagaacagct tgagcagccc cgacttcagg cccgtcacct tgccggccag
     6301 gctgcgcagc gtgtagctcg ggcggtcgtc gatcgcctgg aacgagttct cgggccagag
     6361 ttcgagcgcc acgccggtga aggacctgga cacgaccggc caggcgagcc gacgctcgcc
     6421 gaccgcgggg tcgacgatca cgacgtggcg cggcgtcgcg cgcttgagca ccacgaagtg
     6481 cttcatctcc cagtgcagga tgcacggcgt cctgatctgc gccaggccgt cgagctcgac
     6541 cttgacgggg cgcgcctgca gcgacaggcg ccgcgccatg tcgatcagcg tgcggaaggt
     6601 gacgcccttg ctcgagatgg ggaagcggcg ccgcgccgag gtcatgtcga tctcgtgacc
     6661 gtggaaggcg ccgaccatcg cgacgcaggc gagtccgcat tcggaggcct cggcctgcag
     6721 gaagacgggc agcgcgcggc gcgcgaagaa gccggattgg atgagcttgc gcacggccct
     6781 acgtcccgct cgtcaggaag gtctcgaccg ggaagatgag ctggtacacc ttgcgtgtct
     6841 cgcccatgac cgtcgcatcg acggtggtgc ccggcttgag cgcctcgtcc gcgttgccca
     6901 cccgcacggt ctgccccttg agcgccacgc ggatgcggaa cttgtacggg aagtcgcggc
     6961 cgcgggtggc gcccgactcg aagaactgca ggtcttcctg ccgcagggcg acttgcgaga
     7021 tctcctgcac gacgcccggg aactgtccgt acttctggta cgggaacgcg tgatagcgga
     7081 tcttgaccgg catgccgacc ttgacgttgc cgatcgcgtc gggcggcgca tagagcgtga
     7141 cgccgagcac cccgtcggcc ggcagcaggg tcgccagcac ctggttggcg atgaccggct
     7201 ggcccacttc caccgtgacc ccggacagca cgccgtcccg cgcggcgacg acctgaacca
     7261 gcttgttctc ctcgcgttcg atcatcgcct ggtcgatttc ggtcgtgtcg cgctcggccg
     7321 agcgcgcgtc ctgctgcgcc ttgttcctga gcgccgcaag ccggctctgc acgtcgtcgc
     7381 gatcgatgcg ggcgctggcc tgctggtgcg ccaggtcctc gcccttgagc ttctggtcga
     7441 gaagatcggc ggtcagggcg cgcgtcttgg cgcccgacat gaagccggcc ttctcgagct
     7501 gcgtgttgcg agacaggtcc gattcggcca gcttgatgcg ctcctgctgc agctgcacct
     7561 gctggccgag ccggtcgatc tccacgtcga ggctcgagat gcgatgggcg agcgccagca
     7621 tgtcgttggc atcctgctgg ctctgcagcc tggcctgctc cgcgaggctc tgcttgcgcg
     7681 aggtgagcag gccctcgatc cggccttcgc gcgcggcatt ggagcggtct ccgcgctggg
     7741 tgctcacctc gaacagcacc tgcccggcct tgacgcgctg gccttcgccg acctgcacgc
     7801 caacgaccgt cccgtcgagc agcgagcgta cgccgacggc gccgttgacc ggcaccacgg
     7861 cgcccgagat ctgcgtcttg cgggcgacgt cggccgtcag gacgaagtac atcgcgacgc
     7921 tgaagatcac cgcgctggcg taggcgacca gggtcagcgt gaactgcgaa tcgagcacca
     7981 gcgaaccgat gctgccgttc tgcgcatgct gctgcgcttc ctcccggaac aagtggacga
     8041 tctttgacgg aggcaaggtc tggcggtctt gaaatgggtg ggcggaaagc gccgcccagc
     8101 acgctcggag gcgcgcagtc tagccgccga ctcgtgcgat ccccggcaat gtccgatgcg
     8161 gagaagtacc gatagacggt ggccggccga agcgtggatc ggcatacggc acaccgtatg
     8221 cgcgccgtca tcgaatcgac atgcagggcg gcatccccgg gacgggcgcg gccggacgag
     8281 tcttgcgtca cgcgttggcg cgaacgcgaa tggtgcttgc gatcagttct aggggttgcg
     8341 cattggtggt gcgccggcca gatggccggg gccgacgacg catttggcgg ttcgttcgcc
     8401 gcgacctccc gaggcgccgg gtctcgaacg tcgagccact tcaaatggcc cttgtcccca
     8461 cgcccgacgg atcgcccgaa ccggcggcca ttgctaacat cctcgcctcg acgatacgtg
     8521 caggagtatc cgcatgccct ccaaggccga cgccggcgcc gcgatcacgc gcttccttca
     8581 acgacccaat tcgcgcaccg acttcctcgg cagcttcctg gtctgcgtcg accttcccga
     8641 ggacgacgcg ctgc
//
LOCUS       DATCRO010000505         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_568017, whole genome shotgun sequence.
ACCESSION   DATCRO010000505 DATCRO010000000
VERSION     DATCRO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568017"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VIN75_13830"
     CDS             complement(<1..379)
                     /locus_tag="VIN75_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975298.1"
                     /translation="MARPTKGVQVLVLGGAGLAAFIAAAHLGGRTQDDGTTVVPTVRG
                     ERAAAQAEARPGSTRGVIMPAPAESAASTAHADRGGAIAGDMGQPFVSTSWLPPPPPP
                     PKPVVVVAAPPAAPVAPPLPFTFV"
     gene            complement(379..924)
                     /locus_tag="VIN75_13835"
     CDS             complement(379..924)
                     /locus_tag="VIN75_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975299.1"
                     /translation="MTQAARRIWLKLVRRVGHAGIAGGIALLAAVALAAGLPRLQREV
                     RESDAARSARALAVQQHAQARPVSLLPADVARHYVDGFPAMNRNADDLAQIFAAAQGH
                     HVQLTKGEYQLKADPGAAFVVYTATFPLHGDYDAIKGFSADVLDALPHVAMDELHLSR
                     DNAGSTALDAVVRFTFYYRSL"
     gene            complement(921..1460)
                     /locus_tag="VIN75_13840"
     CDS             complement(921..1460)
                     /locus_tag="VIN75_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975300.1"
                     /translation="MIAHRIPIDFAPGTHGAARRGRLLLLAGVALLGLVLWQGGRLVA
                     ERGAALAALRDIEADRQRAAAPAPAGKPDPRQAALARSTRQVAATLNTPWAGLLGSLG
                     STARQDVALLGVEPSVAKRSVRLTAEARDEAAMLAWLAALQHDARLSSVVLVSHQVQA
                     QAPGTPIRFQVQAQWGDAP"
     gene            complement(1457..2251)
                     /locus_tag="VIN75_13845"
     CDS             complement(1457..2251)
                     /locus_tag="VIN75_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975301.1"
                     /translation="MRLRVGADAVKASAHAGWPRATTLVEAWRAVASPARVATDPSAH
                     APEIDAALAELGSRMPLDGARLEVHLASALLHFDVVEGDFGAQTDRQLQGVAAACVSE
                     MLGDEAAGRAVRWHLQDDDRHLLIVALAQAWIETLQSAATATGLRLASVQPALVARWN
                     ESGKAMKPGMGVFAACDPRDLAIAAVADGAIAAISVGDGVELSVGPGAAGRRGMAAVD
                     DRVDRFLSARGQDPEAQSSFVLVAPHRNADAASTRWSVIASPEMRP"
     gene            complement(2256..3950)
                     /locus_tag="VIN75_13850"
     CDS             complement(2256..3950)
                     /locus_tag="VIN75_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8975302.1"
                     /translation="MDSATTPSIRLDAALVQQAIAACPAGSRPQWLAQRLGADEPAAL
                     ARFADHFRLRPLDMAALRQLRPDFDLISFADSQRRGCVAGRCADDELLAVVTDPTDAR
                     LLQWLDSRPALRSAPRALAIGADLRAFLATAEKTMRAMDGVRLRADSASAAAAAPGSA
                     LSISMAQIDATESPVVRLVDSTLYDALRAGASDIHLETQPGGMTIKYRLDGVLEVVKQ
                     VDSLEFAHRTISRIKVMSELDIAEHRVPQDGRFQVNVDDREIDFRVSIMPNLFGEDAV
                     LRVLDRKHLTGQFKALTLDTLGFAPRIRAFIRDAARMPYGLLLVTGPTGSGKTTTLYA
                     ALSEINTGLDKIVTIEDPIEYQLAGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGE
                     IRDAETAQIAVQAALTGHQVFTSVHANNVFDVIGRFANMGVDSYSLVAALNGIVAQRL
                     LRMACPNCAEACTPDAELLRRSGLPDAALDGASFRRGLGCATCRGTGYKGRLAIAETL
                     PMSDVLREQLVQRASLSQIRQTARQGGFTSLRDAAVALARQGETTLEEINRVTPVE"
     gene            complement(3958..>5017)
                     /locus_tag="VIN75_13855"
     CDS             complement(3958..>5017)
                     /locus_tag="VIN75_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8975303.1"
                     /translation="GPAGSSPVVSARHMDIAGLSYELSSLMGAGLSVMDSLRTLARKE
                     RHGGRRAALLDVVHGVDEGLPLSAALARHPGRFPPLLVATVGASEQTGDLGVALRRYA
                     DHQQSVKALRDKVVGAAVYPLLLLAVGFGVVLFLLGVVVPKFAVLIESSHAQLPWTSQ
                     LMMSWGRLVAAHIGLVGACGAALLAAVVMGARRVLRTGARGRWIEAMPFVGSTIRQFR
                     HAQLYRTTGMLVQGGIPAPRALGLSTSLLGEADQERLRKAIVLLREGRSVSGALDDAG
                     LADPVAASMLTVAERTGALPEILERIAQFHESGLQRSVELTTRLFEPILMVGIGLVVG
                     AIIVMMYLPIFDLASSLQ"
BASE COUNT          692 a         1816 c         1856 g          653 t
ORIGIN      
        1 cgacgaaggt gaacggcagc ggcggggcga cgggcgccgc cggcggcgcg gccaccacga
       61 cgaccggctt gggcggcggc ggtggcggcg gcagccagct ggtcgacacg aagggctggc
      121 ccatgtcccc ggcgatcgcg ccgccccggt ccgcatgcgc ggtggatgcg gccgattcgg
      181 cgggcgcggg catgatgacg ccgcgcgtgg accccgggcg ggcctcggcc tgcgccgcgg
      241 cccgctcgcc gcggaccgtg ggcacgacgg tcgtcccgtc gtcctgcgtc cgcccaccga
      301 ggtgggccgc ggcgatgaac gcggccaggc ccgcgccacc cagcacgagg acctgcacac
      361 ccttggtcgg ccgcgccatc acaggctccg gtagtagaag gtgaagcgca cgacggcgtc
      421 gagcgcggtg ctgcccgcgt tgtcgcgcga caggtggagt tcgtccatcg cgacgtgcgg
      481 cagggcgtcg aggacgtcgg cgctgaagcc cttgatcgcg tcgtagtcgc cgtgcagcgg
      541 gaacgtcgcc gtgtagacga cgaacgcggc gcccggatcg gccttcaact ggtactcgcc
      601 cttggtcagc tgcacgtggt ggccctgtgc ggccgcgaag atctgggcca ggtcgtccgc
      661 gttgcggttc atcgccggga agccgtccac gtagtggcgc gcgacgtcgg ccggcaacag
      721 cgacacgggc cgcgcctgcg cgtgctgctg caccgcgagg gcgcgcgcgc tgcgcgcggc
      781 gtcgctttcg cgcacctcgc gttgcaggcg tggcagcccg gcggccaggg cgacggccgc
      841 cagcagcgcg atgccgcccg cgatgccggc gtggcccacg cgccgcacca gcttcagcca
      901 gatcctgcgt gcggcctgcg tcatggcgca tctccccact gcgcctgcac ctggaaccgg
      961 atcggcgtcc ccggtgcctg cgcctgcacc tggtgcgaga ccaggacgac ggacgacagc
     1021 cgtgcatcgt gctgcaacgc cgccagccag gcgagcatgg ccgcttcgtc gcgggcctcc
     1081 gcggtcagcc gcaccgaccg cttggcgacg gacggctcga cgcccagcag cgcgacgtcc
     1141 tggcgcgcgg tcgaaccgag cgaaccgagc aggcccgccc agggcgtgtt cagcgtggcg
     1201 gccacctgtc gcgtgctgcg cgccagcgcc gcctggcgcg gatcgggctt gccggcgggc
     1261 gcgggcgctg ccgcgcgctg ccggtcggcc tcgatgtcgc gcagcgccgc cagcgccgca
     1321 ccgcgctcgg ccacgagccg gccgccttgc cacagcacca ggcccagcaa ggcgacaccg
     1381 gccagcagca gcaggcgacc ccggcgcgcc gcaccgtggg tgcccggcgc gaaatcgatg
     1441 gggatgcggt gggcgatcac gggcgcatct ccggcgacgc gatcacggac cagcgcgtcg
     1501 aggccgcgtc ggcgttccgg tgcggcgcca ccaggacgaa cgacgactgt gcctcgggat
     1561 cctggccgcg cgcgctcagg aagcgatcga cgcggtcgtc caccgcagcc atgccgcgcc
     1621 ggccggcggc gcccggtccg accgacagct cgacgccgtc gccgacgctg atggcggcga
     1681 tggcgccgtc cgcgacggcg gcgatggcca ggtcgcgcgg atcgcaggcc gcgaagacgc
     1741 ccattcccgg cttcatcgcc ttgcccgact cgttccagcg ggcgaccaac gccggctgga
     1801 cgctcgccag ccgcagtccc gtcgccgtcg cggccgattg cagcgtctcg atccaggcct
     1861 gggcgagcgc cacgatcagc aggtggcgat cgtcgtcctg aaggtgccag cgcacggcgc
     1921 gtccggccgc ttcgtcgccg agcatctcgg acacgcaggc ggcggccacg ccttgcagct
     1981 gtcgatcggt ctgcgcgccg aagtcgcctt ccacgacgtc gaagtgcagc agcgcgctgg
     2041 ccaggtgcac ctcgaggcga gccccatcga gcggcatgcg cgagccgagc tccgcgagcg
     2101 cggcgtcgat ctcgggggcg tgggccgacg ggtcggtggc cacgcgggct ggcgatgcca
     2161 cggctcgcca ggcctcgacg agggtcgttg cgcgcggcca gccggcatgc gcgctggcct
     2221 tgacggcgtc cgcgccgacg cgcagccgca cggatctatt ccaccggggt gacacgattg
     2281 atctcctcga gggtggtctc gccctggcgc gcgagggcga cggccgcgtc gcgcaggctg
     2341 gtgaagccgc cctggcgggc ggtctggcgg atctgcgaca gcgaggcgcg ctgcacgagc
     2401 tgctcgcgca ggacgtcgct catcggcagc gtttcggcga tcgccaggcg ccccttgtag
     2461 cccgtgcccc ggcaggtcgc gcaacccagg ccgcggcgga acgacgcgcc atcgagcgcg
     2521 gcgtccggca ggccgctgcg gcgcagcagc tcggcgtcgg gcgtgcaggc ctcggcgcag
     2581 ttcgggcagg ccatgcgcag caggcgttgc gcgacgatgc cgttcagggc cgcgaccagg
     2641 ctgtagctgt cgacgcccat gttggcgaag cggccgatca cgtcgaagac gttgttggca
     2701 tgcaccgagg tgaagacctg gtggcccgtg agcgcggcct gcacggcgat ctgcgcggtc
     2761 tcggcatcgc ggatctcgcc gaccatgatc ttgtccgggt cgtggcgcag gatcgaccgc
     2821 aggccgcgcg cgaacgtcag gcccttcttc tcgttgacgg gaatctgcag cacgcccgcg
     2881 agctggtact cgatcggatc ctcgatcgtc acgatcttgt ccaggcccgt gttgatctcc
     2941 gacagcgcgg cgtacagcgt cgtcgtcttg ccgctgccgg tcgggcccgt caccagcagc
     3001 aggccgtacg gcatgcgcgc cgcgtcgcgg atgaacgcgc ggatgcgcgg cgcaaagccc
     3061 agcgtgtcca gcgtcagcgc cttgaactgg ccggtcaggt gcttgcgatc cagcacccgc
     3121 agcaccgcgt cctcgccgaa caggttgggc atgatggaca cgcggaagtc gatctcgcga
     3181 tcgtcgacgt tgacctggaa gcgaccgtcc tgcggcacgc ggtgctcggc gatgtcgagt
     3241 tcggacatca ccttgatgcg cgagatcgtg cgatgcgcga actccaggct gtccacctgc
     3301 ttgacgacct cgagcacgcc gtccagccgg tacttgatcg tcatgccgcc gggctgcgtc
     3361 tccaggtgga tgtcgctggc gccggcgcgc agcgcgtcgt acagcgtgga gtcgacgagc
     3421 cggaccacgg ggctctcggt cgcgtcgatc tgcgccatcg agatcgacag cgccgatccc
     3481 ggcgcggcgg ccgcggcgga ggcgctgtcc gcgcgcaggc gcacgccgtc catcgcccgc
     3541 atcgtcttct ccgcggtggc caggaaggcg cgcaggtcgg cgccgatcgc cagggcgcgc
     3601 ggcgcggaac gcagggcggg gcggctgtcc agccactgca gcagccgcgc gtcggtcggg
     3661 tccgtgacga cggccagcag ctcgtcgtcc gcgcagcgtc ctgcgacgca accgcggcgc
     3721 tggctgtcgg cgaacgagat caggtcgaag tcgggccgca gctggcgcag cgcggccatg
     3781 tccagcggcc gcagcctgaa gtggtccgcg aaccgcgcga gggcggccgg ctcgtccgcg
     3841 cccagccgct gcgcgagcca ttgcggccgg cttcccgcgg ggcaggccgc gatcgcctgc
     3901 tggacgagcg cggcatccag gcggatcgag ggggtcgtgg cgctatccat cgcatcctca
     3961 ctgcaggctc gacgcgaggt cgaagatcgg caggtacatc atcacgatga tggcgcccac
     4021 caccaggccg atgcccacca tcaggatggg ctcgaacagg cgcgtcgtga gttcgaccga
     4081 gcgctgcagg cccgattcgt ggaactgcgc gatgcgctcc aggatctcgg gcagcgcgcc
     4141 ggtgcgttcg gccacggtca gcatgcttgc ggcgacgggg tccgcgaggc cggcgtcgtc
     4201 gagcgcgccg ctgacgctgc gaccctcgcg caggagaacg atggccttgc gaaggcgctc
     4261 ctggtcggct tcgccgagca ggctggtcga caggccgagc gcgcgcggcg cggggatgcc
     4321 gccctgcacc agcatgccgg tcgtgcgata gagctgggcg tggcggaact ggcggatggt
     4381 gctgccgacg aaaggcatgg cttcgatcca gcgcccgcgt gcgccggtcc gcagcacgcg
     4441 ccgcgcgccc atgacgacgg cggccagcaa cgccgcgccg caggcgccga cgagcccgat
     4501 gtgcgcggcc acgagccggc cccacgacat catgagctgc gaggtccagg gcagctgcgc
     4561 atgcgagctc tcgatcagca ccgcgaattt cgggacgacg acgccgagca ggaacagcac
     4621 gacgccaaag ccgacggcca gcagcagcaa cggatagacc gcggccccca ccaccttgtc
     4681 gcgcagggcc ttcacgctct gctggtggtc agcgtagcgg cgcagcgcga cgcccaggtc
     4741 gccggtctgc tcgctggcgc cgaccgtggc caccagcagc ggcgggaacc gcccgggatg
     4801 gcgcgctagc gcggcggaca acggcagtcc ttcgtcgacg ccatgcacca cgtccagcag
     4861 cgccgcgcgc cggccgccat ggcgttcctt gcgcgccagc gtgcgcaggc tgtccatcac
     4921 ggagagcccg gcgcccatca gcgacgacag ctcgtacgac aggcctgcga tgtccatgtg
     4981 gcgtgccgac acgacggggc tcgacccggc gggcccc
//
LOCUS       DATCRO010000506         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_984443, whole genome shotgun sequence.
ACCESSION   DATCRO010000506 DATCRO010000000
VERSION     DATCRO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984443"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VIN75_13860"
     CDS             <1..989
                     /locus_tag="VIN75_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8975304.1"
                     /translation="PEEIRRSDMLAWASLTLMGLFSSLFVLTLVRDVVVIALLLARLA
                     GAKVSDHWLDLSASGVVAVALLVTLAGFYNARKAPRVKTVDIPLKDLPPALQGFKIAQ
                     ISDMHVGPTIKRPAMKYVVAKINQLQVDVVAITGDLVDGNVYELSRHVAPLAGLRSSH
                     GTFFVTGNHEYYSGVHPWLKYLPSLGVRVLLNEHVIILHKHAPIVIAGVTDFSAASYD
                     TSHRTDVALAITGAPRAAVKVLLAHQPRSSAAAEQAGFDLQLSGHTHGGQFLPWNFFV
                     RFQQPFTAGLHRVKDMLVYVSRGTGYWGPPKRLGSPSEITLLRLVAQTERRR"
     gene            1053..2171
                     /locus_tag="VIN75_13865"
     CDS             1053..2171
                     /locus_tag="VIN75_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY8975305.1"
                     /translation="MKTVHHALTQGTPGVSIELHSLHFGTPGAGPKATLQAALHADEI
                     PGLLVAHHLRARLAELEAAGRITGEIVLVPVANPIGLAQRVLGSVEGRFELGSGENFN
                     RHYADLFDAVVARVDGRLGADASYNVGLIRAALREAAAAIPARSTLESLRRTLLGLSI
                     DADIVLDLHCDSQAVLHLYTETSVWPQVEPLARFLGCEAALLAQVSGDHPFDEACSTL
                     WPRLAARFGPDTPIPSACVAVTVELRGEADVAHELAERDSQALLDYLAWQGVIAGAPA
                     GMPPARCEATPLAGSIPVNAPHGGVVAYLREPGARVAAGERLADLIEPMTGLVTPLVS
                     PVDGVFYARETRRFVPAGASVLRVAGREALRTGNLLTA"
     gene            2547..3569
                     /locus_tag="VIN75_13870"
     CDS             2547..3569
                     /locus_tag="VIN75_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975306.1"
                     /translation="MSNDPTRAPTRRDAMRGLGASLAAAPLVIVATDSHAQTQQRVDA
                     SRRQGVRDPRNAYPKPPFPVQQQPWPGLARNMNPRPDHGETSYVGSGRLAGRRALITG
                     GDSGMGRAAAIAYAREGADVAINYLPAEEPDAREVVDLIRQAGRKAAAIPGDLRDEGF
                     CRHLVDEAVRELGGLDIVVCNAGRQQARGSILDISTEDFDATMKTNVYAPFWIIKAAL
                     PHLQPGSAIIGTTSEQAYDPSPDLYDYAQTKAATMNYVKSLAKQLGPKGIRVNGVAPG
                     PVWTPLQISGGATEGKVVTFGATAPLGRPGQPAELAGIYVRLAEDDGSFTTGNIYGAG
                     GGQGNP"
     gene            3569..4024
                     /locus_tag="VIN75_13875"
     CDS             3569..4024
                     /locus_tag="VIN75_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY8975307.1"
                     /translation="MLNEGPKTPKPQEFAESAAQSDGYELAAAQAALAQSRHPQVRAF
                     AERMIADHQHMSQSLQEAAKASKLTPPQPHVGGDQAHFLSSLQSLRGGDFDREYGRQQ
                     MLAHTSALAMMRSYADKGSDPNLRRLAATAAPIVERHLQAARQLQESVT"
     gene            4086..4463
                     /locus_tag="VIN75_13880"
     CDS             4086..4463
                     /locus_tag="VIN75_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8975308.1"
                     /translation="MARPDELHDVTGRCACGQVAFTCRIETETCCCSCDLCKRSSGSA
                     FQSWVNGDRASLKVTGRVDSWHSSDHATRHFCPACGSTLFLFEQGEPDVVEVSAGTID
                     APDGILGARHAYAHRRPGWSGQA"
     gene            complement(4566..>5159)
                     /locus_tag="VIN75_13885"
     CDS             complement(4566..>5159)
                     /locus_tag="VIN75_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975309.1"
                     /translation="AMDQPLYVFDRRAPHRPAYALTGIRAFAPEALPQIANAQREAGG
                     AMLPACQPPKPPHMAPGELLLTRRAPSQADPAVACWRILRVATTPTGIEIRADDGDSP
                     RPVKDAPDWSALAARMAALAAPVDAQARAHVTRARAPLRETPDPKSAPRGWLVKGDAV
                     VVLERKAGLAKVLHVGRDGQATERWLAERDIAPDAAR"
BASE COUNT          761 a         1844 c         1827 g          727 t
ORIGIN      
        1 cgcccgagga gatccgtcgc agcgacatgc tcgcctgggc cagcctcacc ctgatgggcc
       61 tgttctcgtc cctgttcgta ctgacgctgg tgcgcgacgt ggtcgtcatc gcgctgctgc
      121 tggcccgcct ggcgggcgcg aaggtgtccg accactggct cgacctgagc gccagcggcg
      181 tcgtcgccgt cgcgctgctg gtgacgctgg ccggcttcta caacgcccgc aaggcgccgc
      241 gcgtcaagac ggtcgacatc ccgctgaagg acctgccccc ggcgctgcaa ggcttcaaga
      301 tcgcgcagat ctcggacatg cacgtcggcc cgacgatcaa gcgaccggcg atgaagtacg
      361 tggtcgccaa gatcaaccag ttgcaggtcg acgtcgtggc catcaccggc gacctcgtgg
      421 acgggaacgt ctacgagctg tcgcgccatg tggcgccgct ggccggcctg cgctccagcc
      481 acggcacctt cttcgtgacg ggcaaccacg agtactactc gggcgtgcat ccgtggctga
      541 agtacctgcc gtcgctgggc gtgcgggtgc tgctcaacga gcacgtgatc atcctgcaca
      601 agcacgcgcc catcgtcatc gcgggcgtca ccgacttctc ggcggccagc tacgacacca
      661 gccaccgcac cgacgtcgcg ctggccatca ccggcgcgcc gcgcgcggcg gtcaaggtgc
      721 tgctggcgca tcagccgcgc agttccgcgg ccgccgagca ggccggcttc gacctgcagc
      781 tgtccggcca cacccacggc ggccagttcc tgccctggaa cttcttcgtg cgcttccagc
      841 agcccttcac cgcgggcctg caccgcgtca aggacatgct ggtctacgtc agccgcggca
      901 ccggctactg ggggccgccc aagcgcttgg gctcgccgtc ggagatcacg ctgctgcgcc
      961 tggtcgcgca gaccgagcgg cggcgctgac ccgcggcctc gcgtcgccgg cgggctgatg
     1021 taacctgtcc gccccgaccc cgcgagaacg ccatgaagac cgtccaccac gcgctgacgc
     1081 aaggcacgcc cggcgtctcg atcgagctgc acagcctgca cttcggcacg cccggcgccg
     1141 gccccaaggc cacgctgcag gcggcgctgc acgccgacga gatccccgga ctgctggtcg
     1201 cgcaccacct gcgcgcgcgc ctggccgaac tcgaggccgc gggccgcatc acgggcgaga
     1261 tcgtgctggt gccggtggcc aacccgatcg gtctggcgca gcgcgtgctc ggctcggtcg
     1321 aagggcgttt cgagctgggc agtggcgaga acttcaaccg gcactacgcc gacctgttcg
     1381 acgcggtcgt cgcgcgcgtc gacggccggc tcggcgccga cgcgtcgtac aacgtcggcc
     1441 tgatccgcgc cgcgctgcgc gaggcggccg cggcgatccc cgcgcgctcg acgctcgaga
     1501 gcctgcgtcg cacgctgctc gggctgagca tcgacgccga catcgtgctg gacctgcact
     1561 gcgacagcca ggcggtgctc cacctgtaca ccgagacgtc ggtctggccg caggtcgagc
     1621 cgctggcgcg cttcctcggg tgcgaggcgg cgctgctggc gcaggtctcg ggcgaccatc
     1681 cgttcgacga ggcgtgctcg acgctgtggc cgcggctggc ggcgcgcttc gggcccgaca
     1741 cgccgatccc gagcgcgtgc gttgccgtca cggtggaact gcgcggcgag gcggatgtcg
     1801 cgcacgagct cgccgagcgt gactcgcagg cgctgctgga ctatctcgcg tggcagggcg
     1861 tgatcgccgg cgcgccggcc gggatgccgc ccgcgcgctg cgaggcgaca ccgctggcag
     1921 gctcgattcc cgtcaatgcg ccgcatggcg gcgtcgtcgc gtacctgcgc gagccggggg
     1981 cgcgggtggc ggcgggcgag cggctggcgg atctgatcga gccgatgacg gggctggtca
     2041 cgccgctcgt gagtccggtc gatggcgtgt tctatgcgcg cgagacgcgg cgcttcgttc
     2101 ctgccggagc gagcgttctg cgcgtcgcgg gccgcgaggc gcttcgcacc gggaacttgc
     2161 tgacggcgtg agttgtgcct ggctgggcgg gcgtggcgcc gttcgcttcg ctgctcgccg
     2221 agcttgcgct ggcggcaggc agggcagggc cgctggcgga tcgctttgcg gacgctggat
     2281 gcaagtccac ggacgaggct tcgcaagcgg gcaaatgacg ctgaaacggc gggatcactt
     2341 ctgtatggaa ggacagcgct ttccttcccc tgatcgcatc gagaggtccc gccaccaacc
     2401 gaacggcact cgtgatgagc acgcgcaatg cgcggcacaa ggcttgccgc gctcctcgcc
     2461 gtagtctggt cggacatggc acgcacggac gggcagcatg accgacaggc acatcccgcg
     2521 accctcgaac accggcagga gcctgcatga gcaatgaccc gacccgcgca cccacgcgac
     2581 gcgacgcgat gagaggcctg ggcgccagcc tcgccgccgc gcccctggtg atcgtcgcca
     2641 ccgacagcca cgcgcagacg cagcagcggg tggacgcctc gcgccgccag ggcgtccgcg
     2701 atcctcgcaa cgcctatccc aagccgccgt tccccgtcca gcaacagccg tggcccggcc
     2761 tggcgcggaa catgaaccct cggcctgacc acggcgaaac cagctatgtc ggatcgggcc
     2821 ggctggcggg ccgcagggcg ctcatcaccg gaggcgactc gggcatgggc cgtgcggcgg
     2881 cgatcgccta tgcgcgcgaa ggtgccgacg tcgccatcaa ctacctgccc gcggaggagc
     2941 ccgatgcgcg tgaggtcgtc gacctgatcc gccaggccgg ccgcaaggcc gccgcgatcc
     3001 cgggcgacct tcgcgacgag ggcttctgcc gccacctggt ggacgaagcg gtgcgcgaac
     3061 tcggcggcct cgacatcgtg gtctgcaatg ccgggcgcca gcaggcgcgc ggctccatcc
     3121 tggacatcag caccgaagac ttcgacgcga cgatgaagac caacgtctat gcgccgttct
     3181 ggatcatcaa ggcggcattg cctcacctgc agccgggctc ggccatcatc ggcaccacct
     3241 ccgagcaggc ctacgacccg tcgcccgacc tctacgacta cgcccagacc aaggcggcca
     3301 cgatgaacta cgtcaagtcg ctggccaagc agctcgggcc gaagggcatc cgcgtcaacg
     3361 gcgtggcacc cggtccggtc tggacgccgc tgcagatctc gggcggcgcc acggagggca
     3421 aggtggtcac cttcggcgca accgcaccgc tgggaagacc cggccagccg gccgagctgg
     3481 ccggcatcta cgtgcgtctt gcggaggacg acggcagctt caccaccggc aacatctatg
     3541 gcgccggcgg cggccagggg aatccgtgat gctgaacgaa ggcccgaaga cacccaagcc
     3601 gcaggagttc gccgagtccg ccgcccagtc cgacggctac gaactggcgg ccgcccaggc
     3661 tgcgttggcg caaagccgcc atccgcaggt gcgcgcgttc gccgagcgga tgatcgccga
     3721 ccaccagcac atgtcgcaat cgctccagga agcggcgaag gcttcgaagt tgacgcctcc
     3781 gcaaccgcat gtgggcggcg accaggcgca ctttctgtcc agcctgcaaa gcttgcgcgg
     3841 cggcgacttc gaccgcgaat acggccgcca gcagatgctg gcccacacca gcgcgctggc
     3901 catgatgcgc agctatgcgg acaaggggtc ggatccgaac ctgaggcgcc tggcagccac
     3961 ggcggcgccg atcgtcgaac gccacctcca ggcggctcgg cagctgcagg aatccgtcac
     4021 gtaggtcggt ggcgggggcc gatcggcatc gacgccgtgt tcgccttcga cgcctggaag
     4081 agccgatggc aaggcccgac gagctccatg acgtgacggg ccggtgcgca tgcggacagg
     4141 tggcgttcac ctgccggatc gagaccgaaa cctgttgctg cagttgtgat ctgtgcaagc
     4201 gcagttcggg cagcgccttc cagtcctggg tcaatggcga tcgtgcgtcc ctgaaggtca
     4261 cgggccgggt cgacagctgg cattcgagcg atcatgcgac gaggcatttc tgccccgcgt
     4321 gcggaagcac cctctttctc ttcgagcaag gcgagcccga tgtcgtcgag gtgagcgcag
     4381 ggaccatcga cgcgcccgac ggcatcctcg gtgcacgcca tgcatacgcc catcggcggc
     4441 ccggctggag cggacaggca tgatgcgcaa tggccgcccg cattcgctgc cgcgcctgga
     4501 cggcgccgcg atgccggcag gcacgcacgg ccacggggac atggaaggcg acgaccgagt
     4561 cgtcgtcagc gcgcggcgtc gggcgcgatg tcgcgctcgg cgagccagcg ctcggtcgcc
     4621 tggccgtcgc ggccgacgtg cagcaccttg gccaggccgg ccttgcgctc gagcacgacg
     4681 accgcgtcgc ccttgacgag ccagccgcgc ggcgcggact tcgggtcggg cgtctcgcgc
     4741 agcggcgcgc gggcgcgcgt gacgtgcgcg cgcgcctgcg cgtccacggg cgcggcgagc
     4801 gccgccatgc gcgcggccag cgcgctccag tcgggcgcgt ccttcacggg gcggggcgag
     4861 tcgccgtcgt cggcgcggat ctcgatgccg gtcggcgtcg tggccacgcg caggatgcgc
     4921 cagcatgcga ccgccggatc ggcttgcgac ggcgccctgc gcgtcagcag cagctcgcct
     4981 ggcgccatgt gcggcggctt gggcggctgg caggcgggca gcatggcgcc gccggcctcg
     5041 cgctgcgcgt tggcgatctg cggcagcgcc tcgggcgcga acgcgcggat acccgtcagg
     5101 gcataggcgg gccggtgcgg cgcgcgccgg tcgaacacgt agagtggctg gtccatggc
//
LOCUS       DATCRO010000507         6482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_917112, whole genome shotgun sequence.
ACCESSION   DATCRO010000507 DATCRO010000000
VERSION     DATCRO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917112"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VIN75_13890"
     CDS             <1..102
                     /locus_tag="VIN75_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adaptor protein RapZ"
                     /protein_id="HEY8975310.1"
                     /translation="GQHRSVFMAETLAQRFSGSVTTLLRHRELDAAE"
     gene            99..614
                     /locus_tag="VIN75_13895"
     CDS             99..614
                     /locus_tag="VIN75_13895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975311.1"
                     /translation="MSAVQARDAVRRVALADWHALPTADRQAVARLEISPEQVEFAGT
                     IARSVAACEAGDPMEVAGFAIRADDAIVGWLLVKRGASAPDWVDADAVVLSGLRIDRR
                     QQGRGIGALALAELARCVVDRWPQAARLVLRVDEGNAAGIRAYQKAGWREVGERRVGR
                     VGLERTMALEF"
     gene            complement(673..1923)
                     /gene="mutY"
                     /locus_tag="VIN75_13900"
     CDS             complement(673..1923)
                     /gene="mutY"
                     /locus_tag="VIN75_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY8975312.1"
                     /translation="MDLFPDDGARRPFIASEATPEWNALPLAERVVAWQRSHGRSGLP
                     WQQTRDPYRVWLSEIMLQQTQVATVLAYYERFLRRFPTVADLAAAPIEDVLALWSGLG
                     YYARARNLHRCAQTVVAEHGGAFPRSASQLAELPGIGRSTAAAIAAFCFGERVAILDG
                     NVKRVLTRVLAFDRDIAQAASEKALWSIATDLLPDAPAGEPLHEAMATYTQGLMDLGA
                     TVCTIRRPACDACPVHAMCQGRASGAPEKFPVKTRKLKRGKRAGVLLWLQDSDRVWLV
                     QRADSGVWAQLWSLPEFDTAVTLERAVEPWPGTLEWMAPFKHVLTHFDWMLQPLRLTW
                     HGGVSPAQQSEIAQLGAVNGASAMAASEPGQADEHGDVEDIDDAERSVATTTTAGRWF
                     TRAELADVGIPSPIRKLLEAALPD"
     gene            complement(2281..4269)
                     /locus_tag="VIN75_13905"
     CDS             complement(2281..4269)
                     /locus_tag="VIN75_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY8975313.1"
                     /translation="MTKDHSLVPALDALASWRQSLARELASLTKFFTTNDLLSAPSAD
                     IAESLRQRLLADKLVVAFVAEFSRGKSELINAIFFADTGRRILPASPGRTTMCPVELA
                     WDDKDAPMLDLLPIDTRKEGQSLTELRATREGWKRVPLDPSNPDALAAALSEVTHTRR
                     VTPDAARELGLWSDTDPEDNPPLDEEGLLEVPAWRHAMINYPHPLLARGLVVLDTPGL
                     NAIGTEPELTLGLLPGAHAALFLLGADAGVTKSDLSVWNEHLGGQSMACFVVLNKVDM
                     LSDPLLTPEQNEALVQKQCEVTAQTLQMPRDRVFPVSARAALHARLNRNEAELEKSRL
                     PQLEEALTNELLPARQTVLTHALVQGVEALRAQVNARLRDQRRQNSEQLLELRGLRGK
                     SGARVRHLLDRVQEDAAEFERCVARLTALRAVQSRLLRDTQNQLSSERIRAEVKELQA
                     ALNSGWLHLGARQAFLGMCDKLRHALSDSEVTTAEAREMLQGTFRQLNAEFGFSLAIA
                     LPPELAQHREDLAAIERGYNRYFGLSETLRMASSAFKERFVRMLVSKLRVVFETAARD
                     IELWNQSASSQMEAQLRDRRRSFRKRRESLERIQQAAGDLEERVTDVEAQSARIEALI
                     TDVGLRCETLCVIARRGPTGIESEHIEIPFDASATATV"
     gene            complement(4533..5420)
                     /gene="mutM"
                     /locus_tag="VIN75_13910"
     CDS             complement(4533..5420)
                     /gene="mutM"
                     /locus_tag="VIN75_13910"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY8975314.1"
                     /translation="MPELPEVEVTRRSVAAGLLGARVRDVQMGLPLRWPLGHDPRALV
                     GTACGEMSRRGKYLWLPLSRDGDGRDRHLPGLPRPADSPALPESAGGLLIHLGMSGAL
                     AFGEPPAERGPYDHFRLVTDRGCLWLTDPRRFGAVVWSPALDAEPARGLLAPLGPEPF
                     DPALDEAGFHAGLKARRTPVKMALLSGELVVGAGNIYACEALHLARIDPRTPSNRVSR
                     PRAARLLAAVRLVLRHAIEAGGTTLRDFSANGAAGHYQESAQVYGREGQPCNTCGTAV
                     RRIVQGQRSTFFCGVCQKR"
     gene            5481..>6482
                     /locus_tag="VIN75_13915"
     CDS             5481..>6482
                     /locus_tag="VIN75_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975315.1"
                     /translation="MKHFSRATRARTTGLLAGLGLALLLPAGAWAQKSAAAPAAPASV
                     PTVPMPVPKVENSYLTGPLLRQLLEAELTMREGQSANAIELMLQAARENKDDALYRRA
                     LQMAAEAGAGDKALAITRSWHAAMPRSTEALLTEIQLLIRFDRIADTAEPLRQLLQIS
                     TPAERVALIASLPRAQQRAKDKGAAAEMFRQLLQPWIDAPETRVAAHVALGRIRLNQG
                     QPMFALDLADQALASDPKALPPVLLALDVMNSATDGTPARASARATAAASAASAAATP
                     EKGTAPSTELIARAEVIVRRYLAQPEAEPVVRQAYASVLATQQRLTDAAEQLRLAVKD
                     "
BASE COUNT          920 a         2310 c         2334 g          918 t
ORIGIN      
        1 gggcagcacc gctcggtctt catggccgag acgctcgccc agcggttctc gggcagcgtc
       61 accacgttgc tgcggcatcg cgagctcgac gcggccgaat gagcgccgtg caagcacgcg
      121 acgccgtgcg acgcgtcgcg ctcgccgact ggcacgccct gccgaccgcc gatcgccagg
      181 ccgtggcgcg gctcgagatc agccccgagc aggtcgagtt cgccggcacg atcgcgcgca
      241 gcgtcgctgc atgcgaggcc ggcgatccga tggaggtcgc cggtttcgcc atccgcgctg
      301 acgatgcgat cgtcggttgg cttctcgtca agcgcggcgc atcggcgccc gattgggtgg
      361 acgccgatgc cgtcgtcctc agcggcctgc gcatcgaccg gcggcagcaa ggccgcggca
      421 tcggcgcgct cgccctggcc gaactggccc gctgcgtcgt cgaccgctgg ccgcaagccg
      481 cgcgcctcgt gctgcgcgtg gacgaaggca acgccgccgg gatccgcgcg taccagaagg
      541 ccggctggcg cgaggtgggc gaacggcgcg tggggcgcgt cgggctcgag cgcacgatgg
      601 cgctcgagtt ctgaacgtct cgcgctcgcg cgttcgcggg cgtcacgcgc gtgcgcgtcg
      661 gcgcgcgtga actcagtccg gaagcgcggc ttccagcagc ttgcggatcg gcgacggaat
      721 gcccacgtca gcgagctccg cgcgcgtgaa ccagcggccc gcggtggtgg tcgtggcgac
      781 gctgcgctcc gcgtcgtcga tgtcctcgac gtcgccgtgc tcgtcggcct ggcccggttc
      841 ggacgccgcc atcgcggatg cgccgttgac cgcgcccagc tgcgcgatct cgctctgctg
      901 cgccggcgac acgccgccgt gccacgtcag gcgcagcggc tgcagcatcc agtcgaagtg
      961 cgtcagcacg tgcttgaacg gcgccatcca ttcgagcgtg ccgggccatg gttcgaccgc
     1021 gcgctccagc gtcacggcgg tgtcgaattc gggcagcgac cacagctgcg cccacacgcc
     1081 cgagtcggcg cgctgcacca gccagacgcg gtcgctatcc tgcagccaca gcagcacgcc
     1141 ggcgcgcttg ccgcgcttga gcttgcgcgt cttgaccggg aatttctccg gcgcgccgga
     1201 cgcgcgaccc tggcacatcg cgtgcaccgg gcatgcgtcg cacgccggcc tgcggatggt
     1261 gcagaccgtg gcgccgaggt ccatcaggcc ctgcgtgtag gtggccatcg cttcgtgcag
     1321 cggctcgccg gccggggcgt cgggcagcag gtcggtggcg atggaccaca acgccttctc
     1381 ggacgcggcc tgcgcgatgt cgcggtcgaa cgcgagcacg cgcgtcagca cgcgcttgac
     1441 attgccgtcg aggatggcca cgcgttcgcc gaagcagaac gcggcgatcg cggcggcggt
     1501 cgaccggccg atgccgggaa gctccgccag ttgcgaggcg cttcgtggga acgcgccgcc
     1561 gtgttccgcg acgaccgtct gcgcgcagcg atgcaggttg cgggcgcggg cgtagtagcc
     1621 gaggccgctc cacagcgcga gcacgtcctc gatcggcgcg gcggcgagat cggccacggt
     1681 gggaaagcgc cgcaggaagc gctcgtagta ggcgaggacg gtggcgacct gcgtctgctg
     1741 cagcatgatc tcggacagcc agacgcggta cgggtcgcgc gtctgctgcc acggcaggcc
     1801 ggaccggccg tgcgaacgct gccaggcgac cacgcgctcg gcaagcggca gcgcgttcca
     1861 ctcgggcgtg gcctcgctgg cgatgaaggg acggcgcgcg ccgtcgtcgg gaaagagatc
     1921 catgccggaa tgaagcggtg tcgcgggagc ggtgtcaggc cttgaatttt gcacgccgcg
     1981 gcgtcggccg atcgtgcggc gctcgccgta cgaaactcaa gacctgacac cgcgctgcaa
     2041 ccgcgacagc gccgaggcgc cggtcaggcg ggtcaagccg caagacctgg caccggatgc
     2101 ccggcgcgtc atcctgcgcg cagtcgcagg atccacctgg cccggccgcg atgccggctc
     2161 ggcgcgcatc cgtggatcct gcgactgcgc gcagggtgac accgggtcgg cgctgcgcgg
     2221 cgatcagccc cgaatccgcg acgactcgcg ccgaacgacg ggctcgcgga cggcgagcgc
     2281 tcagaccgtc gccgtcgccg acgcgtcgaa cgggatctcg atgtgctcgg actcgatgcc
     2341 ggtcgggccg cggcgggcga tgacgcacag cgtctcgcag cgcaggccca cgtcggtgat
     2401 cagcgcctcg atgcgcgcgg attgggcctc gacgtcggtc acgcgttcct cgaggtctcc
     2461 cgcggcctgc tggatgcgct ccagcgattc gcggcgcttg cggaagctgc gccggcggtc
     2521 gcgcagctgc gcttccatct gcgagctggc cgactggttc cacagctcga tgtcgcgcgc
     2581 ggccgtctcg aagacgacgc gcagcttgga cacgagcatg cgcacgaagc gctccttgaa
     2641 cgccgacgac gccatgcgca gcgtctcgct caggccgaag tagcggttgt agccgcgctc
     2701 gatggccgcg aggtcctcgc ggtgctgcgc gagctcgggc ggcagcgcga tggccagaga
     2761 gaagccgaac tccgcgttga gctggcggaa cgtgccttgc agcatctcgc gcgcctcggc
     2821 ggtggtgacc tcggaatcgg acagcgcgtg gcgcagcttg tcgcacatgc ccaggaaggc
     2881 ctggcgcgcc ccgaggtgca gccaaccgga gttcagcgcg gcctgcagct ccttgacctc
     2941 ggcgcggatg cgctcgctgg agagctggtt ctgcgtgtcg cgcagcaggc gcgactgcac
     3001 ggcgcgcagc gcggtcaggc gcgcgacgca gcgttcgaat tcggcggcgt cctcctgcac
     3061 gcgatcgagc aggtgccgca cgcgcgcgcc gctcttgccg cgcaggccgc gcagttcgag
     3121 caattgttcg gaattctggc ggcgctggtc gcgcagccgc gcgttgacct gcgcgcgcag
     3181 cgcttccaca ccttgcacca gcgcgtgcgt cagcaccgtc tggcgcgccg gcagcagttc
     3241 gttggtgagc gcctcttcga gctggggcag gcggctcttc tcgagttcgg cctcgttgcg
     3301 gttcaggcgc gcgtgcagcg cggcgcgggc cgacaccggg aacacgcgat cacgcggcat
     3361 ctgcagcgtc tgcgccgtga cctcgcactg cttctgcacc agcgcctcgt tctgctcggg
     3421 cgtcagcagc gggtccgaca gcatgtcgac cttgttcagc acgacgaagc aggccatcga
     3481 ctggccgccc aggtgctcgt tccagaccga caggtcggac ttggtgacgc ccgcatcggc
     3541 gcccagcagg aacagcgcgg catgcgcgcc gggcagcagg cccagcgtga gttccggctc
     3601 cgtgccgatc gcgttcaggc ccggggtgtc caggacgacc aggccgcgcg ccagcagcgg
     3661 gtgcgggtag ttgatcatcg cgtggcgcca ggccggcacc tcgagcaggc cttcctcgtc
     3721 cagcggcggg ttgtcttccg gatcggtgtc cgaccacagg cccagctcgc gcgcggcgtc
     3781 cggcgtgacg cggcgggtat gggtgacttc cgacagcgcc gccgccagcg cgtccgggtt
     3841 cgacgggtcg agcggcacgc gcttccagcc ctcgcgcgtg gcgcgcagct cggtcagcga
     3901 ctggccttcc ttgcgcgtgt cgatcggcag caggtcgagc atcggcgcgt ccttgtcgtc
     3961 ccacgcgagc tccaccgggc acatggtggt gcggccgggc gaggcgggca ggatgcggcg
     4021 gcccgtgtcg gcgaagaaga tggcgttgat cagctcggac ttgccgcgcg agaactcggc
     4081 cacgaaggcg accaccagct tgtcggccag caggcgctgg cgaagcgatt cggcgatgtc
     4141 ggcggacggc gccgacagca ggtcgttggt ggtgaagaac ttggtgaggc tggccagctc
     4201 gcgggcgagc gattggcgcc agctcgccag ggcgtcgagg gcgggtacca gcgagtgatc
     4261 tttcgtcatt cccaagctct ttgtcgtcgc tagatagtcg gcgccagtgg cggtgattct
     4321 ggttcggaat atggaccagt tgacagcaga tcgtagcgcg attgccctgg agccgatcgc
     4381 ctgattaccc gccggaatgc caagtatgtc gcatcgtgaa gcgtcgccgg gctgctcggc
     4441 ggcgcgtttc ctgctcgacg cgaaacctcc ggatacattc tcggcaaata ctcaccgatg
     4501 agtaatatct ggagcgcttt ggcgcaatat ggctagcgct tctggcaaac cccgcagaaa
     4561 aaggtcgagc gctggccctg cacgatacgg cgcaccgccg tgccgcaggt attgcagggc
     4621 tggccttccc ggccgtacac ctgcgcgctc tcctggtagt ggcccgccgc gccgttggcg
     4681 ctgaagtcgc gcagcgtggt gcccccggct tcgatcgcat ggcgcagcac cagccgcacc
     4741 gccgccagca ggcgcgccgc gcgcgggcgg ctgacgcggt tggacggcgt gcgcgggtcg
     4801 atgcgcgcca ggtgcagcgc ctcgcaggcg tagatgttgc ccgcccccac gaccagctcg
     4861 cccgacagca gcgccatctt cacgggcgtg cggcgcgcct tcagcccggc gtggaagccg
     4921 gcctcgtcga gcgccgggtc gaacggctcg ggtccgagcg gcgccagcag cccgcgcgcc
     4981 ggttcggcat cgagcgccgg cgaccagacg accgcaccga agcgtcgcgg gtcggtcagc
     5041 cacaggcagc cgcgatcggt gacgagccgg aagtggtcgt acggcccgcg ctcggccggc
     5101 ggctcgccga acgccagcgc gccggacatc cccaggtgga tcagcagccc gcccgcggac
     5161 tccggcaagg ccggcgagtc ggccgggcgt ggcaggcccg gcagatgcct gtcccgtcca
     5221 tcgccatcgc gcgacagcgg cagccacagg tacttgccgc ggcgcgacat ctcgccgcac
     5281 gccgtgccca ccagcgcgcg cggatcgtgc ccgagcggcc agcgcagcgg caggcccatc
     5341 tggacgtcgc gcacgcgcgc gcccagcaat cctgcagcaa cgctgcggcg cgtgacttcg
     5401 acctctggca actcgggcat ggtggttctt cggagcctag aatggactga tatggagcgg
     5461 caatgcgtcg aacacaaggc ttgaagcact tttcccgcgc gacgcgtgcg cgcaccacgg
     5521 gcctgctggc cggcctcgga ctcgccttgc ttttgcccgc cggcgcctgg gcgcagaagt
     5581 cggcggccgc cccggccgcg ccggcctcgg tgcccaccgt cccgatgccg gtgcccaagg
     5641 tcgagaactc ctacctcacc ggcccgctgc tcaggcagtt gctggaggcc gaactgacga
     5701 tgcgcgaagg ccagtcggcc aacgccatcg agctgatgct ccaggccgcg cgcgagaaca
     5761 aggacgatgc actgtaccgc cgcgcgctgc agatggcggc ggaagcgggc gccggcgaca
     5821 aggcgctcgc gatcacgcgc agctggcatg cggcgatgcc gcgctcgacc gaggcgctgc
     5881 tcaccgagat ccagctgctg atccgcttcg accgcatcgc cgacaccgcc gagccgctca
     5941 ggcagctgct gcagatcagc acgccggccg agcgcgtggc gctcatcgcc agcctgccgc
     6001 gcgcccagca gcgcgccaag gacaagggcg ccgcggccga gatgttccgc cagctgctgc
     6061 agccctggat cgatgcgccc gagacgcgcg tcgccgctca cgtcgcgctc gggcgcatcc
     6121 gcctgaacca gggccagccg atgttcgcgc tggacctcgc cgaccaggcg ctcgccagcg
     6181 atcccaaggc gctgccgccg gtgctgctcg ccctcgacgt gatgaactcg gccaccgacg
     6241 gcacgccggc gcgcgcgagc gctcgcgcga ccgcggccgc gagcgccgcc agcgcggccg
     6301 ccacgcccga gaagggcacg gcgccgtcca cggagctgat cgcccgcgcc gaggtcatcg
     6361 tgcgacgcta cctcgcacag cccgaggccg agccggtcgt gcgccaggcc tacgccagcg
     6421 tgctcgccac gcagcagcgc ctgaccgacg cggccgagca gctgcgcctg gccgtcaagg
     6481 ac
//
LOCUS       DATCRO010000508         7111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_151992, whole genome shotgun sequence.
ACCESSION   DATCRO010000508 DATCRO010000000
VERSION     DATCRO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151992"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VIN75_13920"
     CDS             complement(<1..1211)
                     /locus_tag="VIN75_13920"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8975316.1"
                     /translation="MKRVDVAVIGAGAAGLFCAAQAGQRGARVLLIDHAAKVAEKIRI
                     SGGGRCNFTNRDATPAQFHSRNPHFCRSALARYTAQDFIALVQRHRIPFHEKHRGQLF
                     CDESSQQIIDLLLRECDDGRVERWQPCAVAGVRARDAGDDGARHELDTARGPVLARSV
                     VVATGGLPVPAIGATDFGLRLARQFGHALVDPRPALVPLVFDAAEWAPFAGLAGVSLE
                     VDISSGTGKQRGHFIEDLLFTHRGLSGPAVLQASSYWRPGETLQIALAPGTDWPDTLA
                     RAKATSRRQLGNELATLLPQRLAQAWLARGGLPEDRPMPEVGDKALRQLAESLASWRI
                     APSGTEGWKKAEVMAGGVDTREIDSRTMESRRAPGLHFIGEVLDVTGWLGGYNFQWAW
                     ASGYACAEALAA"
     gene            complement(1208..1711)
                     /locus_tag="VIN75_13925"
     CDS             complement(1208..1711)
                     /locus_tag="VIN75_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY8975317.1"
                     /translation="MPSYWLMKSEPDECSISDVEKAPGRKVPWIGVRNYQARNFMRDQ
                     MRVGDGVLFYHSSCPEPGVAGVAEVASAAYDDASQFDAKSPYFDARSDPAKPRWQHVD
                     VRHLATTPLLSLKEMRARPELASMQLLKPGSRLSITPVTPEEWRAVMAALVELGAKPS
                     IAKLVAA"
     gene            complement(1751..1935)
                     /gene="ssrS"
                     /locus_tag="VIN75_13930"
     ncRNA           complement(1751..1935)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VIN75_13930"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2028..2393)
                     /locus_tag="VIN75_13935"
     CDS             complement(2028..2393)
                     /locus_tag="VIN75_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY8975318.1"
                     /translation="MKQVEVTILGQSYLLACPDGGEALLQAAVAAVDREMSGIRDAGK
                     VKARERIAVLAALNLAYRLAEAPRAAPVAAVEGGEHAEPKPHVEHAEASEARRAAAAQ
                     VEHLIARLDEALNEDGRLL"
     gene            complement(2390..2641)
                     /locus_tag="VIN75_13940"
     CDS             complement(2390..2641)
                     /locus_tag="VIN75_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HEY8975319.1"
                     /translation="MSRLPDLAERIERLLLRHEEQKRTNTLLEQQLRTVTDERDNLRS
                     RLAAARARIDALIDRLPASEGGHAADDASIDGDSPRSAP"
     regulatory      2793..3056
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3136..5001
                     /locus_tag="VIN75_13945"
     CDS             3136..5001
                     /locus_tag="VIN75_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8975320.1"
                     /translation="MTSRHPGPFAPAHRRVAAFCLAPVTAALSFLAAAPACADPAADP
                     AQEVVVSAARVAQKLPDTLPSTTVISRADIEASPALDVPDLMRSFLSFNVAQTGPVGA
                     QTSIFVRGANSNQVLVLIDGAPLSRADFGSAPWELVPLAQIDHIEVVRGNLSSLYGSA
                     AVGGVIQIFTKHGAGSSLTVGGGSRGTLDGSASVGRRFGADDRALDLSASISGVHTDG
                     WSARDPKVDPSVNPDRDASTQSGATLSVGKTWATGHRTTLSAMHSDTDSHYDGYGGPT
                     VVDELKSRLDNFTLTSHHALTPALNLDVHAGEDVVKYTDPTAYTPGGLGRTRLLGAGV
                     DWTVADGQNVQFGVENDSERFGYVDSPVNSRHTNSVRAGWLGSFASALEVQANVRHDD
                     ASDYGTANTGLLALGWRLGSDWKLVAQASTAFAAPSFSNIAYEAAPLKAEHSRDVELG
                     VHWSRGDWLARATLFSQRQHDLIAFNATFETVNIAHASNRGVELSVDGDTGYGKVGLD
                     ATFQDARDDDAHAALLRRARTVVAANYRVPVMGWDTGVWLQYAGPRADVDPVTFATVQ
                     AHSRTVLGLTASHAFSPNWSIGVKAGNLTGKHDPEVLGYTPPPPSVIFSLHGQWQ"
     gene            5032..5964
                     /locus_tag="VIN75_13950"
     CDS             5032..5964
                     /locus_tag="VIN75_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8975321.1"
                     /translation="MPARDPAVLRIALLALATAVVAALALGLGSGGWDWQLVVTLRAP
                     RVAAAAGVGSLLALAGLALQVLLRNPLADPYVLGHSTGASVGALAALLLGLQLWIGAA
                     SGALVAGLLLLWLARPALAAADDASPKLILLGTMLAAVFGAVSTLMLALVPDQRLRGA
                     IFWMVGDLSGADASLWNIAAAGVFAAWLWTRGRSVDRLMLGADAAWLLGEPVRRLRLE
                     LLVFASLAVGLAVSTAGSVGFVGLVVAQGWRLAGVLRTRELALLSMLSGAALLMGADL
                     LARIVVAPLELPVGAVMALVGAPCFIWFLARGLK"
     gene            5961..6704
                     /locus_tag="VIN75_13955"
     CDS             5961..6704
                     /locus_tag="VIN75_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975322.1"
                     /translation="MSGTPLLEARALTLRAAGRVLVDGLAWRVQAGERWCVIGRNGAG
                     KSLVLRALAGLSVPGRAGELLWQGRPQGDWPAGAAAALRALMPQQPQDRFALTVARVL
                     ALSEVATGAPQASELLSALDIATLAGRRVDQLSGGERQRVALAQCAMQGAGLMLLDEP
                     VSFQDPAHQQVVARWLVQAATRESGARAWIASAHDPSWIARVATHVLALSGDGGWQAG
                     RVEEILSAPVLERTYGCAWRSVEGAWLPA"
BASE COUNT          922 a         2547 c         2577 g         1065 t
ORIGIN      
        1 gccgcgagcg cctcggcgca ggcgtagccg ctggcccacg cccattggaa gttgtagccg
       61 cccagccagc cggtgacgtc cagcacctcg ccgatgaagt gcagcccggg cgcacgtcgg
      121 ctttccatcg tcctggagtc gatctcgcgg gtgtcgacgc cgccggccat gacctcggcc
      181 ttcttccagc cttcggtgcc gctgggcgcg atgcgccacg acgccagcga ctcggcgagc
      241 tggcgcagcg ccttgtcgcc tacctcgggc atcggccggt cctcgggcag tccgccgcgc
      301 gcgagccagg cctgggccag gcgctgcggc agcagggtgg ccagctcgtt gccgagctgg
      361 cgccgggacg tggccttggc gcgcgccaag gtgtcgggcc agtcggtgcc gggcgccaaa
      421 gcgatctgca gcgtctcgcc cggtcgccag tagctcgagg cctgcagcac tgccgggccg
      481 ctcaggccgc ggtgcgtgaa cagcaggtcc tcgatgaaat ggccgcgctg cttgccggtg
      541 cccgaggaga tgtcgacctc cagcgacacg cccgccagcc cggcgaacgg cgcccactcg
      601 gcggcgtcga acacgagcgg caccagcgcc ggccgcggat cgaccagcgc gtggccgaac
      661 tgcctggcca gccgcagccc gaagtcggtg gcgccgatcg ccggcaccgg caggccgccg
      721 gtggccacga ccacgctgcg cgcgagcacc gggccgcgcg cggtgtcgag ttcgtggcgc
      781 gcgccgtcgt cgcccgcgtc acgcgcgcgc acgcccgcga ccgcgcacgg ctgccagcgc
      841 tcgacgcggc cgtcgtcgca ctcgcgcagg agcaggtcga tgatctgctg gctggactcg
      901 tcgcagaaca gctggccgcg gtgcttctcg tggaacggga tgcggtggcg ctgcaccagc
      961 gcgatgaagt cctgcgcggt atagcgcgcc agcgccgagc ggcagaagtg cggattccgc
     1021 gaatggaact gcgccggcgt ggcgtcgcgg ttggtgaagt tgcagcggcc gccgccggag
     1081 atgcggatct tctcggccac cttggccgcg tgatcgatca gcagcacgcg cgcgccacgc
     1141 tgcccggcct gcgccgcgca gaacaggccg gcggcgccgg cgccgatcac ggcgacgtcg
     1201 acgcgcttca agcagcgacc agcttggcga tggacggctt ggcgcccagc tcgaccagcg
     1261 ccgccatcac cgcgcgccat tcctcgggcg tgactggcgt gatcgacagc cggctgcccg
     1321 gcttgagcag ctgcatgctc gccagttcgg gccgcgcgcg catctccttg agcgagagca
     1381 gcggcgtggt ggccaggtgc ctgacgtcga cgtgctgcca gcgcggcttg gccgggtcgg
     1441 acctggcgtc gaagtacggg ctcttcgcgt cgaactgcga cgcgtcgtcg tacgccgcgc
     1501 tggcgacctc ggccacgccc gccacgcccg gctcggggca gctcgaatgg tagaacagga
     1561 cgccgtcacc gacgcgcatc tggtcgcgca tgaagttgcg cgcctggtag ttgcgcaccc
     1621 cgatccacgg caccttgcgg ccgggggctt tctcgacgtc ggagatcgag cactcgtcgg
     1681 gctcgctttt catcagccag tacgaaggca tctgctcgaa agcgtcgtgg cactgcacgc
     1741 cgctgaagta aaggtgtccg ccgcgaacct cgggccgcat tcctgaaccc aggggtgttc
     1801 aggtgggcga ggtcagtctg gcttcgggtt catctgacgc gagacgatca caccaaccag
     1861 acgatgttcc aagccctagc gcaaagcgca tcggttcaag gaaatataaa accctcgcga
     1921 acgcaacgga cgaatccatt ctatgcgccc cgctcgccgc caagcgagca tcagagacgt
     1981 aaagagggcc tacaaaatca cttgacgaac gatcggcgag gaatgcgtta aagcagccgt
     2041 ccgtcctcgt tcagcgcttc gtccaggcgt gcgatcaggt gctcgacctg cgcggccgcg
     2101 gcgcggcgcg cctcgctggc ctcggcgtgt tcgacgtgcg gcttcggctc ggcgtgctcg
     2161 ccgccttcga ccgccgccac cggcgccgcg cgcggtgcct cggcgagacg gtacgccagg
     2221 ttcagcgcgg ccagcacggc gatgcgttcg cgcgccttga ccttgcccgc gtcgcggatg
     2281 ccgctcatct cgcggtcgac cgcggcgacg gcggcctgca gcagcgcctc gccgccgtcg
     2341 gggcaggcca gcaggtagct ctggcccagg atggtcactt cgacttgctt catggcgcgc
     2401 tccgcgggct gtcaccgtcg atggacgcgt catcggccgc atggccgccc tccgacgcgg
     2461 gcaggcggtc gatcagggcg tcgatgcgtg cgcgcgccgc ggccaggcgc gaacgcaggt
     2521 tgtcgcgctc gtcggtgacg gtgcgcaact gctgttcgag cagggtgttc gtccgcttct
     2581 gctcctcgtg gcgcagcagc aggcgttcga tgcgttcggc gagatccggc agtcgtgaca
     2641 tgggcaggga cgcaggggcg gcaaagaccc attgtagggg ccggcttcat gcggccatcc
     2701 gctcgctgac ccttgaacga ggccgtgcgc gatgcctggc tgccgttgcg tccgagccgc
     2761 gcgacatccg ggaacaagag gtgtcgaatg gtacgatccg ctcttcagtt ggtgctccgg
     2821 gcggcctctc gcgccgcccg agtgaaacgg gaatcaggac ggccgccctc gccaggagga
     2881 acgcctcacc tgagctgccc ccgcaacggt cgcggagggt gtcgtcgcct cgagaaggcg
     2941 ccggcatcgg ctgtcgtcac ctggccactg cgcgtctctc gtcgagcgcg tgggaaggcc
     3001 cacgacacgc tgcctctccg ccagcccgga taccggccag ctgttcgggg tcgtccgcgc
     3061 gcaaggcgcc gggcgatcgt ttcggtggca cctgcgggga cgctcgtggc cacgtttgct
     3121 gtcgttcgtg ttcccatgac ctctcgccat cccgggccgt tcgcgcccgc gcaccgccgc
     3181 gtcgcggcgt tctgcctcgc tcccgtcacc gcggcgctgt cgtttctcgc ggcggctccg
     3241 gcctgcgccg atcccgcggc cgatcccgcg caggaagtcg tcgtctcggc cgcgcgcgtc
     3301 gcgcagaagc tgcccgacac gctgcccagc accacggtca tcagccgcgc cgacatcgag
     3361 gcctctcccg cgctcgacgt gcccgacctg atgcgcagct tcctcagctt caacgtcgcc
     3421 cagaccggcc cggtcggggc gcagaccagc atcttcgtgc gcggcgccaa ctccaaccag
     3481 gtgctggtgc tgatcgacgg cgcgccgctg tcgcgcgccg acttcggctc ggcgccgtgg
     3541 gagctcgtgc cgctggcgca gatcgaccac atcgaggtgg tgcgcggcaa cctgtccagc
     3601 ctgtacgggt cggcggccgt gggcggcgtc atccagatct tcacgaagca cggcgccggc
     3661 agcagcctga ccgtgggcgg cggcagccgc ggcacgctcg acggcagcgc gtcggtaggc
     3721 aggcgcttcg gcgcggacga ccgcgcgctg gacctgagcg cgagcatctc cggcgtccat
     3781 accgacgggt ggagcgcgcg cgaccccaag gtcgatccga gcgtcaaccc cgaccgcgac
     3841 gcgtcgacgc agagcggggc gacgctgtcg gtcggcaaga cctgggccac cggccatcgc
     3901 accacgctca gcgcgatgca ctcggacacc gacagccact acgacggcta cggcggcccc
     3961 acggtcgtcg acgagctcaa gagccgcctg gacaacttca cgctgacctc gcaccacgcg
     4021 ctcacgcccg cgctgaacct ggacgtgcac gcgggcgagg acgtcgtcaa gtacaccgac
     4081 cccacggcct acacgccggg cggcctcggc cgcacgcgcc tgctgggcgc gggcgtggac
     4141 tggacggtcg ccgacggcca gaacgtgcag ttcggcgtcg agaacgacag cgaacgcttc
     4201 ggctacgtcg attcgcccgt gaactcgcgc cacacgaact cggtgcgcgc cggctggctc
     4261 ggctcgttcg cctcggcgct cgaggtgcag gccaacgtgc gccacgacga cgcgagcgac
     4321 tacggcacgg ccaacacggg cctgctcgcg ctgggctggc gcctgggctc ggactggaag
     4381 ctggtggcgc aggcctcgac ggcgttcgcg gcgccgagct tctcgaacat cgcctacgag
     4441 gccgcgccgc tcaaggccga gcactcgcgc gacgtcgagc tgggcgtgca ctggagccgc
     4501 ggcgactggc tcgcgcgggc gacgctgttc tcgcagcgcc agcacgacct gatcgcgttc
     4561 aacgccacgt tcgagaccgt caacatcgcc cacgcgagca accgcggcgt ggagctgtcc
     4621 gtcgacggcg acaccggcta cggcaaggtg ggcctggacg ccacgttcca ggacgcgcgc
     4681 gacgacgacg cgcacgccgc gctgctgcgc cgcgcacgca ccgtggtcgc tgcgaactac
     4741 cgcgtgccgg tcatgggttg ggacacgggc gtgtggctgc agtacgccgg cccgcgcgcc
     4801 gacgtcgacc cggtcacctt cgccaccgtg caggcccatt cgcgcacggt gctggggctg
     4861 acggcgtcgc acgcgttctc gccgaactgg tccatcggcg tgaaggcggg caacctcacc
     4921 ggcaagcacg acccggaggt gctgggctac acgccgccgc cgccgtcggt gatcttctcg
     4981 ctgcacgggc agtggcaata atcggctgac cgtcccgtcc gccccacgtc catgcccgcc
     5041 cgcgaccccg ccgtcctgcg catcgccttg ctggcgctcg cgacggcggt ggtggccgcg
     5101 ctcgcgctgg gactgggctc gggtggctgg gactggcagc tggtcgtcac gctgcgcgcg
     5161 ccgcgcgtgg cggcggccgc cggcgtgggc agcctgctcg cgctcgccgg cctcgcgctg
     5221 caggtgctgc tgcgcaatcc gctggccgat ccgtacgtgc tgggacactc taccggcgcg
     5281 tcggtgggcg ccctggcggc gttgctgctg ggcttgcagc tgtggatcgg cgcggcctcg
     5341 ggcgcgctcg tcgcgggact gctgctgctg tggctcgcgc gtcccgcgct ggccgcggcc
     5401 gacgacgcgt cgcccaagct gatcctgctg ggcacgatgc tggccgccgt cttcggcgcc
     5461 gtctccacgc tgatgctcgc gctggtgccc gaccagcggc tgcgcggcgc gatcttctgg
     5521 atggtgggcg acctgtcggg cgccgacgcc agcctgtgga acatcgccgc cgcgggcgtc
     5581 ttcgccgctt ggctgtggac gcgcggccgc tccgtcgacc gcctgatgct gggcgccgac
     5641 gcggcctggc tgctgggcga gccggtgcgc cggctgcggc tcgaactgct ggtgttcgcg
     5701 tcgctggccg tggggctggc ggtgtcgacc gcgggctcgg tcggcttcgt cggactcgtc
     5761 gtcgcacagg gctggcggct cgcgggcgtg ctgcgcacgc gcgaactggc gctgctgtcg
     5821 atgctgtccg gcgcggcgct gctgatgggc gccgacctgc tggcgcgcat cgtcgtcgcg
     5881 ccgctggaac ttccggtggg tgccgtcatg gcgctggtcg gcgcgccatg cttcatctgg
     5941 ttcctcgcgc gaggcctcaa gtgagcggca cgcccttgct cgaggcccgg gcgctgacgc
     6001 tgcgcgccgc cggccgcgtg ctggtcgacg gccttgcgtg gcgcgtgcag gcgggcgagc
     6061 gctggtgcgt gatcgggcgc aacggcgccg gcaagagcct ggtgctgcgc gcgctggcgg
     6121 gcctgtccgt ccccgggcgc gcgggcgagc tgctgtggca gggccggccg cagggcgact
     6181 ggccggccgg cgccgcagcg gcgctgcgcg cgctgatgcc gcagcagccg caggaccgct
     6241 tcgcgctgac ggtggcgcgc gtgctggcgc tcagcgaggt cgccaccggc gcgccgcagg
     6301 cgtccgaact gctgtctgcg ttggacatcg ccacgctggc cggccgccgt gtcgaccagc
     6361 tctccggcgg cgagcgccag cgggtcgcgc tggcgcaatg cgcgatgcag ggcgccggct
     6421 tgatgctgct ggacgagccg gtgtcgttcc aggatccggc gcaccagcag gtcgtcgccc
     6481 gctggctggt gcaggccgcg acgcgcgagt ccggagcgcg ggcctggatc gccagcgcgc
     6541 acgacccgag ctggatcgcg cgcgtcgcca cgcacgtgct ggcgctgtcg ggcgacggcg
     6601 gctggcaggc gggccgcgtc gaggagatcc tcagcgcgcc ggtgctcgag cgcacctatg
     6661 gctgcgcgtg gcgcagcgtc gagggcgcct ggttgccggc ttagggcctg ttcacactaa
     6721 atgcgcgccc gcgccggtgt cctgcgagtc cccagggcta ggcggacgcg acgcggcggg
     6781 ctgggcgccc gcaaggagcg gccaacgacg cctggggact cgcaggccac cggccctccg
     6841 ggtggccgag ctgcaatgcc ggccttcggc gttgcgggcg cttgtagtgg gcccaccatg
     6901 actgcgcgcc cgcgccttga aggcgggcat tctgcttggc cacgcgggcg tgcatttagt
     6961 gtgaacaggc cctaggtgtg aagcttcaat aggttgttgc gggtgttcac ccggagtggt
     7021 tttgagagac tgggaatcgc caaaccccct gtcagaccaa agccggagtc cggatgaaca
     7081 cccataagca tgcccgattg acgtttctga g
//
LOCUS       DATCRO010000509         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_635168, whole genome shotgun sequence.
ACCESSION   DATCRO010000509 DATCRO010000000
VERSION     DATCRO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635168"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VIN75_13960"
     CDS             complement(<1..904)
                     /locus_tag="VIN75_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HEY8975323.1"
                     /translation="MNTAVLDSPAAAVADRRAVLHRLLDEALAYGPEHGAGMASHLPM
                     ALAALDGLGAHEAQMRSFFAHYARRLRRVELPKGGAQPVDDWKRLRGRIDQHETLRAS
                     FARAIARDGADAVLRDAVPGLVDGVAGAAFHGPIRAAHAVEAGHDGELAAGLAYWAAR
                     WEPLPAPGAATLRIAGASAWLDAIDARRITFDPDWRASASLISARMQQAAVTPPYIEL
                     GGALAVEDVAPDALLLDLAKACAARYAATGNFTVLHMATASRALRVLGRWLPVNPVAL
                     APLLHAVAAAELAARAVPLPRDASG"
     gene            977..1480
                     /gene="soxR"
                     /locus_tag="VIN75_13965"
     CDS             977..1480
                     /gene="soxR"
                     /locus_tag="VIN75_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEY8975324.1"
                     /translation="MALPDTDPLAPSQNDWLPIGAFARRAGVSASTLRFYEAQGLMHG
                     GRSESGRRRYPRSALRRVAFIRVAQQVGLTLEQIAAALATLPDGRTPTPADWARLSAA
                     WRPQLDARIAALTRLRDQLDSCIGCGCLSLQRCALYNAGDRAADSGSGARFLLLDGER
                     HPPPGRP"
     gene            1507..1812
                     /locus_tag="VIN75_13970"
     CDS             1507..1812
                     /locus_tag="VIN75_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY8975325.1"
                     /translation="MRLLTWLIRAFLFLALFGLAINNQQLATVNWLFSYAWTARMVII
                     VLAAFTAGTVFGVLAMTPSWWKHRRRARRLETPVVPPPPALPAAPVAPDVTVVRDGL"
     gene            1802..2974
                     /gene="lapB"
                     /locus_tag="VIN75_13975"
     CDS             1802..2974
                     /gene="lapB"
                     /locus_tag="VIN75_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY8975326.1"
                     /translation="MDFEYQWLLLGLPLFFILGWLGSRFDLRQMRRETRESPKAYYKG
                     LSLLLNEQQDKAIDAFIETVQSDPDTTELHFALGNLFRRRGEFERSVRVHQHLLVRAD
                     LPQAERERAQHALAQDFVKAGLFDRAEAAYKALEGTAYHTEARLALLNLYERSRDWRA
                     AIDTAQQLETAGTGSFARRISHYWCELAQAAEDQHHPDEAERALQQALKADPSSARPL
                     VMQGQRLARAGQPAQAFEAWSELRRRDPSAFTLVAGDYAAAAIAAGRGADAKATLEKL
                     LADAPRLDFLAAWARLEADPEVVANRYREMLHAHPTLSAAREVLQQPAIAASAAAVGP
                     LAERSLADVRTAVTRAARPLQRYRCAACGFEAQRHFWQCPGCLSWDSYPATRIEEQ"
     gene            3078..>3642
                     /locus_tag="VIN75_13980"
     CDS             3078..>3642
                     /locus_tag="VIN75_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8975327.1"
                     /translation="MTDRHILCMKWGDKYGPEYVNRLYAMVRRHLRGDFKFVCLTDSS
                     AGIREEVTCLPIPDLALPDGLPERGWKKLTTFEADLHGLRGVALFLDIDIVIVDDITP
                     FFEMPGDFLIIHDWKRPWRVTGNSSVYRFQLGAHADVLAKFRAESAAMRKLYRNEQAY
                     LSGVLHEQGKLQYWPKAWCASFKYHAIA"
BASE COUNT          502 a         1385 c         1255 g          500 t
ORIGIN      
        1 cgcccgatgc atcgcgcggc agcggcaccg cacgcgcggc cagttccgcg gcggccactg
       61 cgtgcagcag cggcgcgagc gcgaccgggt tcaccggcag ccagcgcccg agcacgcgca
      121 gcgcgcggct ggcggtggcc atgtgcagga cggtgaagtt gcccgtggcc gcatagcgcg
      181 cggcgcaggc cttcgccaga tcgagcagca gcgcgtccgg cgccacgtcc tcgactgcga
      241 gcgcgccccc gagctcgatg tacggcggcg tcaccgccgc ctgctgcatg cgcgcgctga
      301 tcagcgacgc ggacgcccgc cagtccggat cgaacgtgat gcgccgcgca tcgatcgcgt
      361 ccagccatgc gctcgcgccg gcgatgcgca gggtcgcggc gcccggcgcc ggcagcggtt
      421 cccaacgcgc ggcccagtat gcgagccccg cggcgagttc gccgtcgtgg cctgcctcga
      481 ccgcatgcgc ggcgcggatc ggcccgtgga aggccgcacc ggcgacgccg tcgacgagcc
      541 ccggcaccgc gtcgcgcagc acggcgtcgg cgccgtcgcg ggcgatcgcg cgcgcgaagc
      601 tggcgcgcag cgtctcgtgc tggtcgatac gaccgcgcag ccgcttccag tcgtcgaccg
      661 gctgggcgcc gcccttgggc agctcgacgc gccgcagccg ccgcgcgtag tgcgcgaaga
      721 acgagcgcat ctgggcctcg tgcgcgccca ggccgtcgag cgcggcgagc gccatcggca
      781 ggtggctggc catgcctgcg ccatgttcgg ggccgtaggc cagcgcctcg tccagcaggc
      841 gatgcaggac ggcgcggcgg tcggcgacgg cggcggcggg cgaatcgagg acggcggtgt
      901 tcatgtcggt cggcacgttg agtggggatg gctacactct gcaagttgaa gtgaacttca
      961 agtcaagcga tttcgcatgg ccctgcccga caccgacccg ctcgcgccca gccagaacga
     1021 ctggcttccc atcggcgcct tcgcccgccg cgccggcgtc agcgccagca cgctgcgctt
     1081 ctacgaggcc cagggcctga tgcacggcgg ccgcagcgag tcggggcgac ggcgctaccc
     1141 gcggtcggcg ctgcgccgcg tcgcgttcat ccgcgtcgcg cagcaggtgg gtctcaccct
     1201 ggagcagatc gccgcggcgc tggccacgct gcccgacggc cgcacgccga cgccggccga
     1261 ctgggcgcgc ctgtcggccg cctggcgccc gcagctggac gcccgcatcg ccgcgctgac
     1321 gcggctgcgc gaccagctcg attcctgcat cgggtgcggc tgcctgtcgc tgcagcgctg
     1381 cgccctgtac aacgccggcg accgggccgc ggactcgggc agcggcgccc gcttcctgct
     1441 gctcgacggc gagcggcacc cgcctcccgg gcgcccgtag aatccgcgaa ccggcggatc
     1501 ctcatgatgc gacttctcac ctggctcatc cgagccttcc tcttcctggc cttgttcggc
     1561 ttggcgatca acaaccagca gctggcgacg gtgaactggc tgttctcgta cgcctggacg
     1621 gcacggatgg tcatcatcgt gctggccgcg ttcaccgccg gcaccgtgtt cggcgtgctg
     1681 gcgatgacgc ccagctggtg gaagcaccgc cgccgcgcgc gccgcctcga gaccccggtc
     1741 gtgcccccgc cgccggccct tcccgccgcg cccgtggcgc ccgacgtcac cgtggtgcgc
     1801 gatggacttt gagtaccaat ggctcctgct gggcctgccg ctgttcttca tcctcggctg
     1861 gctcggttcg cgcttcgacc tgcgccagat gcggcgcgag acgcgtgaat cgcccaaggc
     1921 ctactacaag ggcctgtcgc tgctgctcaa cgaacagcag gacaaggcga tcgacgcgtt
     1981 catcgagacg gtgcagagcg accccgacac caccgagctg cacttcgcgc tcggaaacct
     2041 gttccggcgc cgcggcgagt tcgagcgctc ggtgcgcgtg caccagcacc tgctcgtgcg
     2101 cgccgacctg ccgcaggccg agcgcgagcg cgcccagcac gcgctggcgc aggacttcgt
     2161 caaggccggc ctgttcgacc gcgccgaggc ggcctacaag gcgctcgagg gcaccgcgta
     2221 ccacaccgag gcgcgcctgg ccctgctgaa cctgtacgag cgctcgcgcg actggcgcgc
     2281 cgccatcgac acggcgcagc agctcgagac cgccggcacc ggctcgttcg cgcgccgcat
     2341 ctcgcattac tggtgcgaac tcgcgcaggc cgcggaggac cagcaccacc ccgacgaggc
     2401 cgagcgcgcg cttcagcagg cgctcaaggc cgatccgtcg tcggcgcgcc cgctggtgat
     2461 gcagggccag cgcctcgcgc gtgcggggca gcctgcgcag gccttcgagg cctggagcga
     2521 gctgcgccgg cgcgatccgt ccgcgttcac gctggtggcc ggcgactacg ccgcggccgc
     2581 catcgccgcc ggccgcggcg ccgatgccaa ggccacgctc gagaagctgc tggccgacgc
     2641 gccacgcctg gatttcctgg ccgcctgggc caggctcgag gccgaccccg aggtcgtcgc
     2701 caaccgctac cgcgagatgc tgcacgcaca tccgacgctg tcggccgcgc gcgaggtgct
     2761 gcagcagccg gcgatcgccg cctcggccgc cgccgtcggc ccgctggccg aacgctcgct
     2821 ggccgacgtg cgcaccgccg tcacccgcgc cgcgcgcccg ctgcagcgct atcgctgcgc
     2881 cgcctgcggc ttcgaggccc agcgccactt ctggcaatgc cccggctgcc tcagctggga
     2941 cagctacccc gccacgcgga tcgaagaaca atgacccgat atttccgcac cacgccggcc
     3001 gagcgctcgg ccgctccgga gccggccggc atccccccgg gggatcgaac gatgtacccg
     3061 tcgggcgagg ggcggcaatg accgacaggc acatcctgtg catgaaatgg ggcgacaagt
     3121 acggccccga gtacgtcaat cgcctctacg cgatggtccg ccgccacctg cgcggcgact
     3181 tcaagttcgt ctgcctgacc gactcgagcg ccggcatccg cgaggaagtg acctgcctgc
     3241 ccatccccga cctggcgctg cccgacggcc tgcccgagcg cggctggaag aagctgacca
     3301 ccttcgaggc cgacctgcac gggctgcgcg gcgtggcgct gttcctggac atcgacatcg
     3361 tcatcgtcga cgacatcacg ccgttcttcg agatgcccgg cgacttcctg atcatccacg
     3421 actggaagcg cccgtggcgc gtcaccggca attcctcggt ctaccgcttc cagctgggcg
     3481 cgcacgccga cgtgctggcc aagttccgcg cggaatcggc ggcgatgcgc aagctctatc
     3541 gcaacgagca ggcctatttg tccggcgtgc tgcatgagca gggcaagctg cagtactggc
     3601 ccaaggcgtg gtgcgccagt ttcaagtacc acgccatagc ga
//
LOCUS       DATCRO010000510         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_557336, whole genome shotgun sequence.
ACCESSION   DATCRO010000510 DATCRO010000000
VERSION     DATCRO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557336"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1346
                     /locus_tag="VIN75_13985"
     CDS             42..1346
                     /locus_tag="VIN75_13985"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8975328.1"
                     /translation="MPDLIVHGGRPLSGRITPSANKNAVLPILSATLLTREPVTLRGV
                     PDITDVRKILAVFRELGSHVEGELKDGVLRIHHEHTHFDPAVHKLPEEMRSSVMLVPP
                     LLARFGSARIENDVQGCTLGVREIDPHVEVFEGFGAQVERGPGALIVHARRPLHAHRR
                     WLDYASVTTTENFVLCAALADGVSELTNAASEPHVQEFCDFMQRMGAKVEGIGTSRLT
                     VTGVDELHGVDFTFVEDFHEVVTFLALGAITGGNVEVRNSAPQFFPLLDRSFAKLGVE
                     VTHEGGWSRAYVPAGLKVREPFTRNMLQKIEAAPWPYFPVDLLPIFIALGVRAEGQMM
                     FWNKVYDGALGWTSELSKFGAHAFQSDPHRVITFGGKPLVGAEVDSPYIIRVAIALLM
                     LASSIDGKSTIYNGTPIRRAHPRFVENLVSLGANIEWVGPEN"
     gene            complement(1358..1765)
                     /locus_tag="VIN75_13990"
     CDS             complement(1358..1765)
                     /locus_tag="VIN75_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY8975329.1"
                     /translation="MSVRIVRLGTPRHPHEGTRIGTVRRPPRGVPKSEFASGDWYDVW
                     YPNLSPTPELVKRALASQADLSAAGEKEWAAFTRGYKAEMKEPENARTIELLAALSQH
                     ANFSVGCYCENEARCHRSILRGLLQEAGARMDE"
     gene            complement(1792..2265)
                     /locus_tag="VIN75_13995"
     CDS             complement(1792..2265)
                     /locus_tag="VIN75_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8975330.1"
                     /translation="MIRIGLISDTHGLLRPAAVDVLRGSDFIVHAGDIGENVLEALST
                     IAPVTAVRGNNDRAAWAERIHETEQLRFGQITLHVLHDLAQLAIDPNAAGVDVVVTGH
                     SHRPKIERRSGVLYVNPGSAGPVRFNLPVSVALMQVSGRDVNVRIVELESLRRTA"
     gene            complement(2353..>3988)
                     /locus_tag="VIN75_14000"
     CDS             complement(2353..>3988)
                     /locus_tag="VIN75_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8975331.1"
                     /translation="AMLRVLGQTQRRIAAVHAIEFVALGGVASLLGLALGFALHLGFV
                     ALLGAWLPTGGLPWPGAQPALLGLGVGLTLLLGFGLPPVLQLARVPPLRVLRRDVGTP
                     KAASLGVLAGAFLGFAALMVIAARDVKLGLISVGGFAAAALAFALVGWLAVRALRRLV
                     PEARAPRWLVLATRAIAARPAFAVLQVASMAIGLMALWVLVLLRTDLIDSWRAATPPD
                     APNRFVVNIQPDQADAYRAMLKSAGVRGDDWFPMIRGRLVSINDEPVGKRYKPDSDAS
                     NMIQRELNLSHSAQVQDKVRIVAGTWVPDEPDGLSVEEGLARTLGLKLGDRMVFDVGG
                     QTVSGRVTTLRKVDWGSMRVNFFVMFPRASMGDLPLSYISAFKAPAKPGFDNQLAAQF
                     PNVTAIDVSATIAQVQGVLDQVIHAVELLFAFALVAGLVVLVATVSATREARAREFAV
                     MRAVGASSALLGRVQQVELAGIGALAGLLAASAAVAVGWSLATWVFEFHWNPPWWVPV
                     AGALSGGVLALGAGWWSLHEVLARPVMETLRRAAQE"
BASE COUNT          610 a         1463 c         1367 g          548 t
ORIGIN      
        1 acgatggcgc cacgtttcca acaagcctca agagagttca cgtgcccgat ctcatcgtcc
       61 atggcggccg ccccctcagc ggccgcatca cgccctccgc gaacaagaac gcggtgctgc
      121 cgatcctgtc ggccacgctg ctgacgcgcg aaccggtgac cctgcgcggc gtgcccgaca
      181 tcaccgacgt ccgcaagatc ctcgccgtgt tccgcgaact gggcagccat gtcgaaggcg
      241 agctcaagga cggcgtgctg cgcatccacc acgagcacac ccacttcgat ccggccgtgc
      301 acaagctgcc cgaggagatg cgctcgtccg tgatgctggt gccgccgctg ctggcgcgct
      361 tcggcagcgc gcgcatcgag aacgacgtgc agggctgcac gctgggcgtg cgcgagatcg
      421 atccgcacgt cgaggtgttc gagggcttcg gcgcgcaggt cgagcgcggc cccggcgcgc
      481 tgatcgtgca cgcccgccgc ccgctgcacg cgcatcgccg ctggctggac tacgcctcgg
      541 tcaccaccac cgagaacttc gtgctgtgcg ccgcgctggc agacggcgtg tcggagctga
      601 ccaacgccgc gtccgagccg cacgtgcagg agttctgcga cttcatgcag cgcatgggcg
      661 cgaaggtcga gggcatcggc acctcgcggc tgaccgtcac cggcgtcgac gagctgcacg
      721 gcgtcgactt caccttcgtc gaggatttcc acgaggtcgt gaccttcctg gcgctcggcg
      781 cgatcacggg cggcaacgtc gaagtgagga acagcgcgcc gcagttcttc ccgctgctcg
      841 atcgcagctt cgccaagctg ggcgtggagg tcacgcacga gggcggctgg tcgcgcgcgt
      901 acgtgcccgc cggcctgaag gtgcgcgagc cgttcacgcg caacatgctg cagaagatcg
      961 aggccgcgcc gtggccctac ttcccggtcg acctgctgcc catcttcatc gcgctgggcg
     1021 tgcgcgccga gggccagatg atgttctgga acaaggtcta cgacggcgcg ctgggctgga
     1081 cgtccgagct gtcgaagttc ggggcccacg cgttccagtc cgacccgcac cgcgtcatca
     1141 ccttcggcgg caagccgctg gtgggcgccg aggtcgacag cccgtacatc atccgcgtcg
     1201 cgatcgcgct gctgatgctg gcctccagca tcgacggcaa gtcgacgatc tacaacggca
     1261 cgccgatccg ccgggcgcat ccgcggttcg tggagaacct ggtgtcgttg ggggcgaaca
     1321 tcgagtgggt cggaccggaa aactgacggc cccacgctca ttcgtccatc cgcgcccccg
     1381 cctcctgcag caggccgcgc aggatggagc gatggcatcg cgcctcgttc tcgcaatagc
     1441 atccgaccga gaagttcgcg tgctgcgaca gcgccgccag cagctcgatc gtgcgggcgt
     1501 tctccggctc cttcatctcg gccttgtagc cgcgcgtgaa cgcggcccat tccttctcgc
     1561 cggcagcgct caggtcggcc tgcgacgcga gcgcgcgctt gacgagttcg ggcgtgggcg
     1621 agaggttcgg gtaccagacg tcgtaccagt cgccggaggc gaactccgat ttcggcactc
     1681 cgcgcggcgg gcgccgcacg gtgccgatgc gcgtgccctc gtgcgggtgg cgcggcgtgc
     1741 ccaggcggac gatgcggaca ctcatggcgg cggtctcctc gatgcgggct gtcacgccgt
     1801 gcggcgcaac gactccagtt ccacgatgcg cacgttgacg tcgcggccgc tgacctgcat
     1861 cagcgccacc gacaccggca agttgaagcg caccggcccg gcgctgcccg gattgacgta
     1921 gaggacgccg ctgcgtcgct cgatcttcgg gcggtgggag tggcccgtga cgacgacatc
     1981 gacgcccgcg gcattcggat cgatcgccag ctgcgcgagg tcgtgcaaca cgtgcagcgt
     2041 gatctgcccg aagcgcagtt gctccgtctc gtggatgcgc tcggcccagg ccgcgcgatc
     2101 attgttgcca cgcacggcgg tcaccggcgc gatggtggac aacgcctcga gcacgttctc
     2161 gccgatgtcg ccggcgtgga cgatgaagtc gctgccgcgc aggacgtcga ccgccgcggg
     2221 gcgcagcagg ccgtgggtgt cggagatgag gccgatccgg atcatggcgt caacccatca
     2281 atcggcgtgc cggcgccgct gcgcgcgcgg gctcgcgctc gcgccgcgcc tgacgcctgc
     2341 gcgtccggag gctcactcct gcgcggcacg acgcaaggtc tccatcacgg gacgtgccag
     2401 cacttcgtgc aggctccacc agccggcgcc gagcgccagc acgccgccgc tcagcgcgcc
     2461 ggccacgggc acccaccagg gcgggttcca gtggaattcg aacacccagg tcgccaggct
     2521 ccagccgacg gccaccgccg cgctggcggc gagcaggccg gccagcgcgc cgatgccggc
     2581 cagctcgacc tgctgcacgc ggcccagcag ggcactcgac gcgcccaccg cgcgcatcac
     2641 cgcgaactcg cgcgcccgcg cctcgcgcgt ggccgacacc gtggccacca gcaccaccag
     2701 gcccgccacc agcgcgaacg cgaacagcag ctccaccgca tggatgacct ggtcgagcac
     2761 gccctgcacc tgcgcgatgg tggccgacac gtcgatcgcc gtgacgttgg gaaattgcgc
     2821 cgccagctgg ttgtcgaagc cgggcttggc cggcgccttg aacgcgctga tgtagctcag
     2881 cggcaggtcg cccatgctgg cgcgcgggaa catcacgaag aagttcaccc gcatcgagcc
     2941 ccagtcgacc ttgcgcagcg tggtgacgcg gcccgagacg gtctgaccgc cgacgtcgaa
     3001 caccatccgg tcgccgagct tgaggcccag cgtcctggcc aggccctcct cgacgctcag
     3061 gccgtccggc tcgtcgggca cccaggtgcc ggcgacgatc ctgaccttgt cctgcacctg
     3121 cgcggagtgg ctcaggttca gttcgcgctg gatcatgttg gacgcgtcgg agtcgggctt
     3181 gtagcgcttg cccaccggct cgtcgttgat gctgacgaga cggccgcgga tcatcgggaa
     3241 ccagtcgtcg ccccgcacgc cggcggactt gagcatggcg cggtacgcat cggcctggtc
     3301 gggctggatg ttgacgacga agcggttggg cgcgtccggc ggcgtggcgg cgcgccagct
     3361 gtcgatgaga tcggtgcgca gcagcaccag cacccacagc gccatcaggc cgatcgccat
     3421 cgaggccacc tgcagcaccg cgaacgccgg gcgcgcggcg atcgcgcgcg tggccaggac
     3481 aagccaccga ggtgctcgcg cctcgggcac gaggcgccgc agcgcgcgca ccgccagcca
     3541 gcccacgagc gcgaacgcca gcgccgccgc cgcgaagccg cccacgctga tgagccccag
     3601 cttgacgtcg cgcgccgcga tcaccatcag cgccgcgaag cccaggaacg cgcccgcgag
     3661 gacgccgagg ctggccgcct tgggcgttcc cacgtcgcgc cgcagcacgc gcagcggcgg
     3721 cacgcgcgcc agttgcagca ccggcggcag gccgaaaccg agcagcagcg tcaggcccac
     3781 gcccaggccc agcagcgccg gctgcgcgcc cggccacggc agcccgcccg tcggcagcca
     3841 tgcgccgagc agcgcgacga agcccaggtg cagcgcgaag cccagcgcca ggcccagcag
     3901 gctggcgacg cctcccagcg cgacgaactc gatcgcatgc acggcggcga tgcggcgctg
     3961 cgtctgaccg agcacccgca gcatcgcg
//
LOCUS       DATCRO010000511         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_230227, whole genome shotgun sequence.
ACCESSION   DATCRO010000511 DATCRO010000000
VERSION     DATCRO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230227"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3021)
                     /locus_tag="VIN75_14005"
     CDS             complement(<1..3021)
                     /locus_tag="VIN75_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8975332.1"
                     /translation="MWMTRVSINNPVFATMVMVALCVLGLFSYQRLGVEQMPDISPPV
                     VFIAVQYPGASPGAVETEITKPLEDALNGIAGVKMIRSNSYEGRSETVVEFHLSVDMT
                     RATQEVRDKVSVVQAGFNRDVKPPFVSRFDGDNSQPTVVLALMANGRSDRELSLIADQ
                     TVQKRLTSVEGVARVTPLGLVTRQVRIDLDPQRLRGFGLTPADVSAALVRANTDQPVG
                     LLQNRNEDSLVRVEGRVLDPKQFANVVVSRNASGGVVLLGDVGKLVEREQEPDSLSRI
                     NGKAAVTFNIFKQQDANIVRTGAAIKAAADEMRKSLPPGVELKLIYASSDWVEQSLDG
                     VKKTLGEGALLTIAIVFLFLHSWRSTIITGLTLPISVIASFIAINAFGFTLNFMTMMA
                     LSLCIGLLIDDAIVVRENIVRHVGMGQDHHTAARRGTDEIGLAVMATTFAICAVFVPV
                     AFMKGIVGKFFFPFGITVVSAVIVSLFVSFTLDPMLSSVWKDPPAVTMQRIWGIGHMM
                     RAVDWAMARLHHIYERLITWAFSGRRYRMFVPPVPAYGFGFGADGRRLVHGKRHLRLA
                     TLTPRGIVLWSAVGTFFAAIALATTIGAEAIPQTDQSYTGLQITMPVGTSLARADEKM
                     KQVEAIIGSMPEIDSLSTQVGGDNGHNTAYIGIGLKPRHERKLNQFQVEDEIRKRLEG
                     LPGISVALGNRPIFISILGPDPTVLDQITADLGAKIRKVKGVTDFESSVKPGLPAYAV
                     RVRPDAARQLGMTPQQLSASLRAYVQGDVSTYWTTPDGQQVEVQLRLPQASRETVAQL
                     NKLPVAFAADGTPVPLESVATIEPVTNPDVIKRQDLQRRQAVYAGVDVKSGRTVGQVN
                     ADIDKIVKATVLPPGYRFDVGGAAQDQDDIFGNIGSALGLALIFIYIVLASQFGSFLQ
                     PLAIMASLPLSLIGVMISLKAFHSTINLFSLIGLIMLMGLVTKNAILLVDFANHARKA
                     GATLAEAVLTAGQIRMRPIIMTTAAMVFGMFPLAL"
     gene            complement(3062..4144)
                     /locus_tag="VIN75_14010"
     CDS             complement(3062..4144)
                     /locus_tag="VIN75_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8975333.1"
                     /translation="MAANTPGAAASEVPLDFTPNEAARPLQLALPQVVEFSGPLVAPN
                     TAIVRAKATGTLLSLDVQEGQRVKAGEPLGRIDVSDLQSKVAERVANLAAVKTTLAQA
                     QRTHDSNIGLANDKFISPIALENSKSALDSAKAQVAAAEATLDSARIALRDASLVAPI
                     SGIVSKKYVVSGEKVTAEQQVVTIVDLHLLELAGTVGTQEVGQLTTGMPVTLKIEGLD
                     KGIDGKLARIAPAAEAGTRSIGVVVQLQNPKETMRAGQYAVAQVLIGDATPRLTVPIS
                     AISSASGQEYVWTVENGKLLRRTVTTGRRDPVRGRAEVLEGLTPDAPVLGARFDNLRE
                     GAKARVVAVATAATPKPLPAAATATR"
     gene            complement(4558..>5142)
                     /locus_tag="VIN75_14015"
     CDS             complement(4558..>5142)
                     /locus_tag="VIN75_14015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8975334.1"
                     /translation="LNPFFAPNAQIIPRLEERMDDLRKAGDSCGARIEVVARGMPVGL
                     GQPLFDKLDADIAYAMMGVNAVKGVEIGAGFASVAQRGTEHGDELTPEGFVGNNAGGV
                     LGGISTGQDLRVSIAIKPTSSIRTPRRSIDRAGNPAVVETFGRHDPCVGIRATPIVEA
                     MLALVVMDHVLAHRAQCGDVHLEQPPIPGAVPRA"
BASE COUNT          756 a         1713 c         1866 g          807 t
ORIGIN      
        1 gagcgcgagc gggaacatgc cgaacaccat cgcggccgtc gtcatgatga tcgggcgcat
       61 ccggatctgg cccgcggtca gcacggcctc ggccagcgtc gccccggcct tgcgggcgtg
      121 gttggcgaag tcgaccagca ggatggcgtt cttggtcacc aggcccatca gcatgatcag
      181 gccgatcagc gagaacaggt tgatggtgga gtggaaggct ttcagcgaga tcatcacgcc
      241 gatcagcgac agcggcagcg aggccatgat cgccagcggc tgcaggaagc tgccgaactg
      301 gctggccagc acgatgtaga tgaagatcag cgccaggccc agggccgagc cgatgttgcc
      361 gaagatgtcg tcctggtcct gcgcggcgcc gcccacgtcg aatcggtagc ccggcggcag
      421 cacggtggcc ttgacgatct tgtcgatgtc ggcgttgacc tggcccaccg tgcggcccga
      481 cttgacgtcc acgccggcgt acaccgcctg gcggcgctgc aggtcctggc gcttgatcac
      541 gtccgggttg gtgacgggct cgatcgtggc gacgctctcc agcggcaccg gcgtgccgtc
      601 ggcggcgaag gcgaccggca gcttgttgag ctgcgcgacc gtctcgcggc tggcctgcgg
      661 caggcgcagc tgcacctcca cctgctggcc gtcgggcgtc gtccagtagg tcgacacgtc
      721 gccctgcacg taggcgcgca ggctggccga cagctgctgc ggcgtcatgc ccagctggcg
      781 cgcggcgtcc ggacgcacgc gcacggcgta cgcgggcagg ccgggcttga ccgaggattc
      841 gaagtcggtc acgcccttga ccttcctgat cttggcgccg aggtcggccg tgatctggtc
      901 gagcacggtc ggatccgggc ccaggatcga gatgaagatc gggcggttgc ccagcgccac
      961 cgagatgccc ggcaggccct ccaggcgctt gcggatctcg tcttccacct ggaactggtt
     1021 gagcttgcgc tcgtggcgcg gcttcaggcc gatgccgatg taggcggtgt tgtggccgtt
     1081 gtcgccaccg acctgcgtgg acagcgagtc gatctcgggc atcgagccga tgatggcctc
     1141 gacctgcttc atcttctcgt cggcgcgcgc caggctggtg cccacgggca tcgtgatctg
     1201 caggccggtg tagctctggt cggtctgcgg gatcgcttcc gcgccgatcg tggtggccag
     1261 cgcgatggcc gcgaagaagg tgcccacggc cgaccacagc acgatgccgc gcggggtcag
     1321 cgtggcaagg cgaaggtggc gcttgccgtg caccaggcgg cggccgtcgg cgccgaagcc
     1381 gaagccgtac gccggcaccg gcggcacgaa catgcggtag cggcggccgc tgaaggccca
     1441 ggtgatcagg cgctcgtaga tgtggtgcag cctggccatg gcccagtcga cggcccgcat
     1501 catgtggccg atgccccaga tgcgctgcat cgtgacggcc ggcggatcct tccagaccga
     1561 cgacagcatc gggtcgagcg tgaagctgac gaacagcgac acgatcaccg cggacaccac
     1621 cgtgatgccg aacgggaaga agaacttgcc gacgatgccc ttcatgaagg ccaccggcac
     1681 gaacaccgcg cagatcgcga aggtggtggc catcaccgcc aggccgatct cgtcggtgcc
     1741 gcggcgcgcg gccgtgtggt ggtcctggcc catgccgacg tgccgcacga tgttctcgcg
     1801 caccacgatc gcgtcgtcga tcagcaggcc gatgcacagc gacagcgcca tcatcgtcat
     1861 gaagttcagc gtgaagccga acgcgttgat cgcgatgaac gaggcgatga ccgagatcgg
     1921 cagcgtcagg ccggtgatga tggtcgagcg ccagctgtgc aggaacagga acacgatggc
     1981 gatcgtcagc agcgcgccct cgcccagcgt cttcttgacg ccgtcgagcg actgttccac
     2041 ccagtcggag ctggcgtaga tcagcttgag ctcgacgccc ggcggcagcg acttgcgcat
     2101 ctcgtcggcc gcggccttga tcgccgcgcc ggtgcgcacg atgttggcgt cctgctgctt
     2161 gaagatgttg aacgtgactg cggccttgcc gttgatgcgc gagagcgaat cgggctcctg
     2221 ctcgcgttcg acgagcttgc cgacatcgcc caggagcacc acgccgccgc tggcgttgcg
     2281 cgagaccacc acgttggcga actgcttggg atcgagcacg cggccttcga cgcgcaccag
     2341 cgaatcctcg ttcctgttct gcagcaggcc gaccggctgg tcggtgttgg cgcgcacgag
     2401 cgcggcgctg acgtcggccg gcgtgaggcc gaagccgcgc aggcgctgcg ggtcgaggtc
     2461 gatgcgcacc tggcgcgtga ccagccccag cggcgtgacg cgagcgacgc cctcgacgct
     2521 ggtcaggcgc ttctgcaccg tctggtcggc gatcagggac agctcgcggt cgctgcggcc
     2581 gttggccatc agcgcgagca ccacggtggg ctgcgagttg tcgccgtcga agcggctgac
     2641 gaacggcggc ttgacgtcgc ggttgaagcc ggcctgcacc accgagacct tgtcgcgcac
     2701 ttcctgcgtg gcgcgcgtca tgtcgacgga caggtggaac tcgacgaccg tctcgctgcg
     2761 gccctcgtag ctgttggagc ggatcatctt gacgccggcg atgccgttca gggcatcctc
     2821 cagcggcttg gtgatctcgg tctcgacggc gccgggcgac gcgcccgggt actgcacggc
     2881 gatgaagacc accggcggcg agatgtcggg catctgctcg acgcccaggc gctggtacga
     2941 gaacaacccg agcacgcaca acgccaccat gaccatggtc gcgaagacgg ggttgttgat
     3001 ggagacacgc gtcatccaca tgggaactct cctcgagtcg aagccgtggg gacggcgcgg
     3061 atcaacgggt ggcggtggcg gcggcgggca gcggcttggg cgtggcggcg gtcgcgaccg
     3121 cgaccacccg cgccttggcg ccctcgcgca ggttgtcgaa gcgcgcgccc agcaccgggg
     3181 cgtcgggcgt caggccctcg agcacctcgg cgcgtccgcg caccgggtcg cggcggcccg
     3241 tggtgacggt gcggcgcagc agcttgccgt tctccaccgt ccacacgtac tcctggccgg
     3301 aggccgagct gatcgcgctg atgggcaccg tcaggcgcgg cgtggcgtcg ccgatgagga
     3361 cctgcgcaac ggcgtactgg ccggcgcgca tcgtctcctt ggggttctgc aactgcacga
     3421 cgacgccgat cgagcgcgtg ccggcctcgg cggccggcgc gatgcgggcc agcttgccgt
     3481 cgatgccctt gtccaggccc tcgatcttca gcgtgacggg catcccggtg gtcagctgcc
     3541 cgacctcctg ggtgccgacc gtgccggcca gctccagcag gtgcaggtcg acgatcgtga
     3601 ccacctgctg ttcggcggtg accttctcgc ccgagacgac gtacttcttg gacacgatgc
     3661 cgctgatggg cgccaccagc gacgcgtcgc gcagcgcgat gcgggcgctg tcgagggtgg
     3721 cctcggccgc ggcgacctgc gccttggcgc tgtccagggc ggacttggaa ttctccagcg
     3781 cgatcggcga gatgaacttg tcgttggcca gcccgatgtt ggagtcgtgc gtgcgctggg
     3841 cctgtgccag cgtcgtcttg accgccgcca ggttggcgac gcgctcggcg accttgctct
     3901 gcaggtcgct gacgtcgatg cggcccagcg gctcgccggc cttcacgcgc tggccttcct
     3961 gcacgtccag cgacagcagc gtgccggtcg ccttcgcgcg cacgatcgcc gtgttgggcg
     4021 cgaccagggg gcccgagaac tcgaccacct gcggcagcgc gagctgcaac ggcctggccg
     4081 cctcgttggg cgtgaaatcg agcggcacct cgctggcggc ggcgccaggt gtgttggcgg
     4141 ccatcgcggc gctcgccggc acggcggcgg cccccctgcc agccttgatc gcgaaacccc
     4201 ctgcgatagc cacgacgatg gccgcgaccc cgaataccaa gcgtttgcgc atgtcctctt
     4261 ccttcgtctt cttttcaggg gcatcctgaa tttcgacgga gtgtggatcg gcgggcaggg
     4321 tcatgggggc ggattgcgac gaagtgctgc tgggtcagga cgagttgcag tgcgcctgga
     4381 tggaccggaa tccgggccga cgaactgcag gaaacgacga tcggccggca tggcgcgcgc
     4441 gacgaaagga cgtcgccggt gcgcgaccgc aagctccgtg aggataagcc gcccgcggac
     4501 ggacgcaaag gcgcgccgta ccccccggac ggggggggcg cggccgtcac gggcgcgtca
     4561 cgcgcgcggg acggcgccgg ggatgggcgg ctgctcgagg tggacgtcgc cgcactgggc
     4621 gcggtgcgcc agtacatggt ccatcaccac cagcgcgagc atcgcctcga cgatgggcgt
     4681 cgcccggatg ccgacgcacg ggtcgtggcg gccgaaggtc tcgaccaccg ccgggttgcc
     4741 cgcgcggtcg atcgagcggc gcggcgtgcg gatcgagctg gtgggcttga tcgcgatcga
     4801 cacccgcagg tcctggccgg tggagatgcc gcccagcacg ccgccggcgt tgttgccgac
     4861 gaagccctcg ggggtcagct cgtcgccatg ctcggtgccg cgctgggcga cgctcgcgaa
     4921 gccggcgccg atctcgacgc ccttgacggc gttgacgccc atcatggcgt aggcgatgtc
     4981 cgcgtcgagc ttgtcgaaca gcggctggcc caggccgacg ggcatgccgc gggcgacgac
     5041 ctcgatgcgc gcgccgcagg agtcgccggc cttgcgcagg tcgtccatgc gctcctcgag
     5101 gcgcgggatg atctgcgcgt tcggggcgaa gaacgggttc ag
//
LOCUS       DATCRO010000512         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_286855, whole genome shotgun sequence.
ACCESSION   DATCRO010000512 DATCRO010000000
VERSION     DATCRO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286855"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VIN75_14020"
     CDS             complement(<1..1419)
                     /locus_tag="VIN75_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8975335.1"
                     /translation="MATYKGITYPDAVAPLARHMPSVNDHREALAALSATWDTLGLLA
                     HLSNLKADMREVRASFGELTGELLACLAEETLALARETLGHQAQIAIDVLTRNLFERT
                     ADIGFLATDAAIVCACLDRDPARLAALRERLRAYAARYTVYSDIVLLGPDGRVLTRLV
                     DGFAGWSSSSVIGRALNGQGGWVETYEATDFCGDALALTHAWRVEHEGRAVGVLALVF
                     DLEREAALVFERLAREDEVIAFVDADDRVLLSNDAARLPAGRRLALRPDAVSLRLGGV
                     THVAARRAGQAYQGYPGPGWAAIALAPVELAFGDDAGDGAPVAFSGENVFSQRLRDVP
                     VRAREIQRRLDRMVWNGRIHQADESNAFSRSLLEEIAATGRRTKDQFERASSQLLATV
                     ASGLQSEARFLAELSVDILARSLYERANDVRWWAADPVLATLDARAARATLARINGLY
                     TVYANLLLFDAQGLVLAASRDAG"
     gene            complement(1497..2561)
                     /locus_tag="VIN75_14025"
     CDS             complement(1497..2561)
                     /locus_tag="VIN75_14025"
                     /EC_number="2.7.7.1"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HEY8975336.1"
                     /translation="MPSDIAVLVGRFQPFHDAHLALLQRALESASRCVVIIGSAFQAR
                     TPKNPFTWKERAEMIRQALPEADRARLVIVPMRDYYDEARWVASVRDAVAALLPPEEA
                     PRVTLVGHLKDATSSYLAAFPGWALASVDRLPGAGGTQLRDALFAAQPADDHTPPAVD
                     AALAAFVDQVPPSTIAFLRAWTAMPHYAELAAEWRMLKRYKEAWRAAPYEPTFVTVDA
                     VVQCAGRVLLIQRGKAPGRGLFAVPGGFIEQRETCWQSCLRELEEETHLKLLDATMRR
                     SLRALAVFDHPDRSQRGRTITHAHHFDLGDRELPEVRADDDAASVQWVPIADLAAMED
                     RFFDDHFHMLDHFLGVTGGA"
     gene            complement(2578..4278)
                     /locus_tag="VIN75_14030"
     CDS             complement(2578..4278)
                     /locus_tag="VIN75_14030"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8975337.1"
                     /translation="MLKITVAQLDYMVGDIAGNVGKMIAAAREAAAAQADLVVFSELS
                     LTGYYPGDLLDEPDFLARAAQGLADLQVASQEFPSLHWIVGAPLEREGAGKRLQNALV
                     VLKAGQVVLRYAKQLLPTYNIFDELRHFAPGPDVARVLRIRGTQVGLMICEDGWNDSG
                     ADYAVNPFSRLADAAPDLVVSINASPSDIGKREMRHQVFGAACRRHGFPLLYVNQIGG
                     QDTVVFDGASFALDARGAVCYEARRFVEDVTTLAFDDGAFTSGSGAPLAPVSPQGLST
                     MAFYRAQIVLGLRDYARRCGFTKAVIGSSGGIDSALTLALAVDALGAENVVAITMPSV
                     YSSAGSVDDSVTLCRNLGVVLHTHPIAHAVAAFAAQFEASFGQPLKGLPLENLQARVR
                     GTTLMEYSNTYGHLLLTTGNKSEISVGYCTLYGDTNGGLGLIGDLYKTEVFALSRHVN
                     EAAGRELIPQAIIDKEPSAELAPGQRDTDSLPPYPVLDEILKLLIEGTRLAPAERARA
                     SEFVTQLRATPEGEALVQRVRGLIARNEYKRRQAPPIIRVRSRAFGSGRQMPIAAKVA
                     "
     gene            complement(4290..>5353)
                     /gene="pncB"
                     /locus_tag="VIN75_14035"
     CDS             complement(4290..>5353)
                     /gene="pncB"
                     /locus_tag="VIN75_14035"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8975338.1"
                     /translation="LDEVNEQLDHLCALSFTEDELAYIGALRYIKNDFVDFLRIFRFQ
                     RRFIRASAAADGRTLEIVAEGPQVHVMGFEIYVLAIVNELYFRRSDQAAALVEGRARL
                     RAKIAALREFGAREPARAHPFEFFDFGVRRRFSGAWQDEVVRTLKAEVPEFFKGTSNV
                     HLARKHGLVAIGTMAHEYLQSYQAFDVRLRDFQKMALENWVQEYRGDLGVALTDVVGM
                     DAFLRDFDLYFAKLFDGLRHDSGDPIEWGEKALAHYAKLRIDAHTKRMTFSDGLDLET
                     AFSIWRHFADRTQLGFGIGTTLTNDVGLVPLNIVMKLTQCNGQPVAKLSDSPGKTLCN
                     DETFLAYLRQVFQVAPQLP"
BASE COUNT          785 a         1836 c         1981 g          751 t
ORIGIN      
        1 gccggcgtcg cggctggcgg ccagcacgag gccttgcgcg tcgaacagca gcaggttcgc
       61 atacacggtg tacaggccgt tgatgcgggc cagcgtcgcg cgggcggcgc gggcgtccag
      121 ggtcgccagc accgggtcgg cggcccacca gcgcacgtcg ttcgcgcgct cgtagaggct
      181 gcgcgcgagg atgtcgaccg acagctcggc caggaagcgc gcctcggact gcaagcccga
      241 cgcgacggtc gccagcagtt gcgacgaagc gcgctcgaac tggtccttgg tgcgccggcc
      301 ggtggccgcg atctcctcca gcagcgagcg cgagaacgcg ttcgattcgt cggcctggtg
      361 gatgcggccg ttccacacca tgcggtcgag gcggcgctgg atctcgcggg cgcgcaccgg
      421 cacgtcgcgc agtcgctgcg agaagacgtt ctccccgctg aacgcgaccg gcgcgccgtc
      481 gcccgcatcg tcgccgaagg cgagctccac cggcgccagc gcgatggcgg cccagcccgg
      541 gcccggatag ccctggtacg cctgcccggc gcggcgcgcc gcgacgtgcg tgacgccgcc
      601 cagccgcagc gatacggcgt ccggccgcag cgcgaggcgg cgcccggccg gcaggcgcgc
      661 ggcgtcgttc gacagcagca cgcggtcgtc ggcgtcgacg aaggcgatca cctcgtcctc
      721 gcgcgccagg cgctcgaaca cgagcgcggc ctcgcgctcc aggtcgaaca ccagcgccag
      781 cacgccgacc gcccggccct cgtgctcgac gcgccaggcg tgggtcaggg ccagcgcgtc
      841 gccgcagaag tcggtggcct cgtacgtctc gacccagccg ccctggccgt tcagcgcgcg
      901 gccgatgacc gacgagctgg accagccggc aaagccgtcg accagccgcg tcagcacgcg
      961 gccatcggga cccagcagca cgatgtcgct gtagacggtg tagcgcgcgg cgtaggcccg
     1021 caggcgctcg cgcagcgcgg ccaggcgcgc gggatcgcgg tcgaggcagg cgcagacgat
     1081 ggcggcgtcg gtcgccagga agccgatgtc ggccgtgcgc tcgaacaggt tgcgcgtgag
     1141 cacgtcgatc gcgatctgcg cctggtggcc cagcgtctcg cgggccagcg cgagcgtctc
     1201 ctcggccagg cacgcgagca gctcgccggt gagctccccg aagctcgcgc gcacctcgcg
     1261 catgtcggcc ttcaggttgg acaggtgcgc cagcagcccc agggtgtccc aggtggcgct
     1321 caacgcggcg agcgcctcgc gatggtcgtt gacggacggc atgtggcgcg cgagcggcgc
     1381 cacggcgtcg gggtaggtga tgcctttgta cgtcgccatg aaggggtgcg atgggctggg
     1441 gcccgcgcga tgttagccct cgcttggccg gtcgttccac ccgtcaaacc cgcggttcag
     1501 gcgccgccag tgactccgag gaagtggtcc agcatgtgga agtgatcgtc gaagaaccga
     1561 tcctccatcg cggcgagatc ggcgatcggc acccattgca ccgacgccgc gtcgtcgtcg
     1621 gcccgcacct cgggcagctc gcggtcgccg aggtcgaagt ggtgcgcgtg cgtgatcgtg
     1681 cggccgcgct ggctgcggtc ggggtggtcg aacacggcca gcgcgcgcag gctgcggcgc
     1741 atcgtcgcgt ccagcagctt caggtgcgtc tcctcctcca gttcgcgcag gcacgactgc
     1801 cagcaggtct cgcgctgctc gatgaagccg ccgggcaccg cgaacaggcc gcggcccggg
     1861 gccttgccgc gctggatcaa cagcacgcgg ccggcgcact gcaccaccgc gtcgacggtg
     1921 acgaaggtgg gctcgtaggg cgcggcccgc cacgcctcct tgtagcgctt gagcatgcgc
     1981 cattcggcgg ccagctcggc atagtgcggc atggccgtcc aggcacgcag gaaggcgatg
     2041 gtggacggcg gcacctggtc gacgaaggcg gcgagcgcgg cgtcgacggc cggcggcgtg
     2101 tggtcgtcgg cgggctgcgc cgcgaacagg gcgtcgcgca gctgcgtgcc gccggcgccg
     2161 ggcaggcggt cgacggaggc cagcgcccag cccgggaagg cggccaggta gctgctggtg
     2221 gcatccttga ggtggcccac cagcgtgacg cgcggcgcct cctcgggcgg cagcagcgcg
     2281 gcgaccgcgt cgcgcaccga cgcgacccag cgcgcctcgt cgtagtagtc gcgcatcggc
     2341 acgatgacca ggcgcgcacg gtcggcctcg ggcagcgcct ggcggatcat ctccgcccgc
     2401 tccttccacg tgaacgggtt cttgggcgtg cgtgcctgga acgcggagcc gatgatgacg
     2461 acgcagcgcg acgccgactc gagcgcgcgc tgcagcagcg ccaggtgcgc gtcgtgaaag
     2521 ggctggaagc gtccgacgag gacggcgatg tcggaaggca tgggcgtcct cgcttggtca
     2581 ggcgaccttg gccgcgatgg gcatctggcg gccgctgccg aacgcgcgcg agcgcacccg
     2641 gatgatgggc ggcgcctggc ggcgcttgta ctcgttgcgc gcgatcaggc cgcgcacgcg
     2701 ctgaaccagc gcctcgccct cgggcgtggc gcgcagttgc gtcacgaact cgctggcgcg
     2761 cgcgcgctcg gccggagcca ggcgcgtgcc ctcgatcagc agcttcagga tctcgtcgag
     2821 caccgggtag ggcggcaggc tgtcggtgtc gcgctgcccc ggcgcgagct cggccgaggg
     2881 ctccttgtcg atgatcgcct gcgggatcag ttcgcggccc gcggcctcgt tgacgtggcg
     2941 cgacagcgcg aacacttcgg tcttgtacag gtcgccgatc aggcccaggc cgccgttggt
     3001 gtcgccgtac agcgtgcagt agcccaccga gatctccgac ttgttgcccg tggtcagcaa
     3061 caggtggcca tacgtgttgg agtattccat cagcgtcgtg ccgcgcacgc gcgcctgcag
     3121 gttctccagc ggcaggccct tgagcggctg gccgaacgag gcctcgaact gcgcggcgaa
     3181 ggcggccacg gcgtgggcga tcgggtgcgt gtgcagcacg accccgaggt tgcggcacag
     3241 cgtcaccgag tcgtccaccg agccggccga tgaatacacg gagggcatcg tgatggcgac
     3301 gacgttctcc gcgccgagcg cgtccaccgc cagtgccagc gtgagcgcgc tgtcgatgcc
     3361 gcccgacgag ccgatcaccg ccttcgtgaa gccgcagcgg cgcgcgtagt cgcgcaggcc
     3421 cagaacgatc tgagcgcgat agaacgccat ggtcgacagg ccctgcggcg agacgggcgc
     3481 cagcggcgcg ccgctcccgg acgtgaaggc gccgtcgtcg aacgcgagcg tggtgacgtc
     3541 ctcgacgaag cgccgcgcct cgtagcagac cgcgccccgc gcgtcgagcg cgaacgaggc
     3601 gccgtcgaag accaccgtgt cctggccgcc gatctggttg acgtacagca gcgggaagcc
     3661 gtggcgcctg caggccgcgc cgaacacctg gtggcgcatc tcgcgcttgc cgatgtccga
     3721 cgggctggcg ttgatggaga ccacgagatc gggcgccgcg tccgccaggc gggagaacgg
     3781 gttgacggcg tagtcggcgc cgctgtcgtt ccagccgtcc tcgcagatca tcaggccgac
     3841 ctgcgtgccc cggatgcgca gcacgcgcgc gacgtccggg cccggcgcga agtggcgcag
     3901 ctcgtcgaag atgttgtagg tgggcagcag ctgcttggcg tagcgcagca ccacctggcc
     3961 ggccttgagc acgaccagcg cgttctgcag ccgcttgccg gcgccttcgc gctcgagcgg
     4021 cgcgccgacg atccagtgca aggacgggaa ttcctgcgac gcgacctgca ggtcggccag
     4081 gccctgcgct gcgcgcgcga ggaagtcggg ctcgtcgagc aggtcgccgg ggtagtaacc
     4141 cgtgagcgag agctcggaaa acacgacgag gtcggcctgc gccgcggcgg cctcgcgcgc
     4201 ggcggcgatc atcttgccga cgttgccggc gatgtcgccg accatgtagt cgagctgggc
     4261 gacggtgatc ttgagcatga tgggtgtgat cagggcaatt gcggtgcgac ctggaagacc
     4321 tggcgcaggt aggcgaggaa ggtctcgtcg ttgcacagcg tcttgccggg cgagtccgag
     4381 agcttggcca ccggctggcc gttgcactgc gtcagcttca tgacgatgtt cagcgggacc
     4441 aggccgacgt cgttggtgag cgtggtgccg atgccgaagc ccagctgcgt gcggtcggcg
     4501 aagtggcgcc agatcgagaa cgcggtctcc aggtcgaggc cgtcggagaa cgtcatgcgc
     4561 ttggtgtggg cgtcgatgcg cagcttcgcg tagtgcgcca gggccttctc gccccactcg
     4621 atcgggtcgc ccgagtcgtg gcgcaggccg tcgaacagct tggcgaagta caggtcgaag
     4681 tcgcgcagga aggcgtccat gccgacgacg tcggtcagtg ccacgccgag gtcgccgcgg
     4741 tactcctgca cccagttctc gagcgccatc ttctggaagt cgcgcaggcg cacgtcgaag
     4801 gcctggtaag actgcaggta ttcgtgcgcc atcgtgccga tcgcgaccag cccatgcttc
     4861 ctggccagat gcacgttgga cgtccccttg aagaactcgg gcacctcggc cttgagcgtg
     4921 cgcaccacct cgtcctgcca agcgccggag aagcgccggc gcacgccgaa gtcgaagaac
     4981 tcgaacgggt gcgcgcgcgc cggctcgcgg gcgccgaact cgcgcagcgc ggcgatcttg
     5041 gcgcgcagcc gcgcacgccc ctcgaccagc gcggcggcct ggtcggaacg ccggaaatag
     5101 agctcgttga cgatcgccag cacgtagatc tcgaagccca tcacgtgcac ctgcgggccc
     5161 tcggcgacga tctccagcgt gcggccgtcc gccgccgccg acgcgcggat gaagcggcgc
     5221 tggaagcgga agatgcgcag gaagtcgacg aagtcgttct tgatgtagcg cagcgcgccg
     5281 atgtaggcga gctcgtcctc ggtgaacgac agcgcgcaca ggtggtcgag ctgctcgttc
     5341 acctcgtcca gca
//
LOCUS       DATCRO010000513         5037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_939571, whole genome shotgun sequence.
ACCESSION   DATCRO010000513 DATCRO010000000
VERSION     DATCRO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939571"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..565)
                     /locus_tag="VIN75_14040"
     CDS             complement(80..565)
                     /locus_tag="VIN75_14040"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8975339.1"
                     /translation="MSGRFTYVTEWRLDAPINQVWDALTDVENWPAWWPHVRSVRTLH
                     PGAPGGLGSVRRLRWSSRLPYGFSLDVETTSMRAPTHLVGRATGDMAGTGTWELACAG
                     AGTRVRYTWTLDLNTRWMRLAAPFMAPVFRWNHEGVMRSGGQGLARHLASRPPLRSLA
                     L"
     gene            complement(562..957)
                     /locus_tag="VIN75_14045"
     CDS             complement(562..957)
                     /locus_tag="VIN75_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8975340.1"
                     /translation="MNDALTLIQDAYGCFGRGDIPALLALTTPDVDWRFHGDHGAGYT
                     GRVIGHAHLAEWFGEVARADDIQSFEPREFLAGPDHVTVIGHERTIARATGRVFETPW
                     VHVWRLREGRIAGFTGLFDTQAVAQARQS"
     gene            complement(1053..1577)
                     /locus_tag="VIN75_14050"
     CDS             complement(1053..1577)
                     /locus_tag="VIN75_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975341.1"
                     /translation="MSAPTFRLADPIADRATLLAINIEYVAWVFAEIEAMSGMTARGI
                     LGAEVSDYVPTVIDKVCGDPPPRGAFYLVEHAGGVVAMGGLRRSEDGVAELKRVYVRP
                     AGRGHRLGEALTRRLLDDARTFGYRTVRLDTLPFMRAAQAVYESLGFVDCAPYPVEMP
                     EVFRSHIRYMALAL"
     gene            1871..2911
                     /gene="pyrC"
                     /locus_tag="VIN75_14055"
     CDS             1871..2911
                     /gene="pyrC"
                     /locus_tag="VIN75_14055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8975342.1"
                     /translation="MTTLNELSIQTPDDWHLHIRDGAAMAAVVPYTARQFGRAIIMPN
                     LRPPITTTAQAVAYRERIVAALPEGSTFEPLMTLYLTDNLPPDEIKRAKDAGIVAVKL
                     YPAGATTNSDAGVTDLRKTYKTLEAMQREGIKLLVHGEVTSAEIDLFDREAVFIDQVF
                     EPLRRDLPELKVVFEHITTREAAQYVAESSAFTAATITAHHLLYNRNAIFLGGLRPHY
                     YCLPVLKREVHRQALVAAATSGSAKFFLGTDSAPHASVMKEASVCGAGCFTALSALEL
                     YAEAFDGVGALDKLEGFASHHGPDFYGLPRNTGTVTLRRESWELPASVPFGEAAVLKP
                     LRAGETLGWKQA"
     gene            2986..3354
                     /locus_tag="VIN75_14060"
     CDS             2986..3354
                     /locus_tag="VIN75_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975343.1"
                     /translation="MRESLERLGRFDPQRARDRFLAAFEPALTRHVLAGGERVGFVVV
                     RPQDDGLLLDHLYLLPTAQGRGVGAAVLADVLARADASRLPVRVGALRGSDSNRFYQR
                     HGFELVEQGEWDNLYLRPVR"
     gene            3449..3794
                     /gene="rnpB"
                     /locus_tag="VIN75_14065"
     ncRNA           3449..3794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIN75_14065"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3847..>5037
                     /locus_tag="VIN75_14070"
     CDS             3847..>5037
                     /locus_tag="VIN75_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY8975344.1"
                     /translation="MNPDAATLWRRPRSALAALLACLGMLGAFSIDTYIPAFAGIARA
                     LDATPVQMQQTLSAYLFGFAVMNLFHGALADSFGRRPVVLWGIAVFTLSSLGCALSQS
                     IGHLVFFRLAQGMSSGASMVVGRAIIRDMFPPDEAQRVMSQVTIYFGVAPAVAPIVGG
                     LLLVHYGWHSIFIFLTGIGVLLWSLSWRLLPETLHHTQVQPFHPAHLLKGYWGMFTSP
                     RFLALALASGVPFNGMFLYILSAPVFLGDLLHLDPTHFFFLFLFTIGGIMGGAFLSGK
                     LAGKMKPARQIRIGFTIMFSVAVLNLVLNSLFTAHAWWAIPPIGVFSFGWALMVPVVT
                     LLVLDQAPLRRGMASSLQACIGSAANGLVAGLLVPMVMHSTVALACASAALMLVGLVA
                     WYGVR"
BASE COUNT          724 a         1776 c         1771 g          766 t
ORIGIN      
        1 cccgtcgcca gggcgcggct gccgtgcgcg atgagatgga aggcaccgtg cggcggctgg
       61 tcgccgcagg ccttgtcgtt cagagcgcga gcgatcgcag cggtggccgg ctggcaaggt
      121 ggcgcgccag cccttgcccg ccgctgcgca tcacgccctc gtggttccag cggaacaccg
      181 gcgccatgaa cggtgcggcc aggcgcatcc agcgcgtgtt caggtccaag gtccaggtat
      241 agcgcacgcg ggtgccggcg ccggcgcagg caagctccca ggtgccggtg ccggccatgt
      301 cgccggtcgc gcggccgacc aggtgcgtcg gcgcgcgcat cgacgtcgtc tcgacgtcca
      361 gcgagaaacc gtagggcagg cggctgctcc agcgcaacct gcgcacgctg cccaggccac
      421 cgggcgcgcc cggatgcagc gtgcgcaccg agcgcacatg cggccaccag gcgggccagt
      481 tctcgacatc ggtcagggcg tcccagacct ggttgatcgg ggcgtccagg cgccactcgg
      541 tgacgtacgt gaagcggccg ctcatgactg gcgggcctgc gcgacggcct gggtgtcgaa
      601 caggccggtg aagcccgcga tgcggccttc gcgcaggcgc cagacatgca cccacggcgt
      661 ctcgaacacg cggccggtgg cacgcgcgat cgtgcgctcg tggccgatga cggtgacgtg
      721 gtccggcccg gcgaggaact cgcgcggctc gaacgactgg atgtcgtccg cccgcgcgac
      781 ctcgccgaac cattcggcca agtgggcgtg gccgatgacg cggcccgtgt agccggcgcc
      841 atggtcgccg tggaaccgcc agtcgacatc gggcgtcgtc agggcgagca gggccgggat
      901 gtcgccgcgg ccgaagcagc cgtaggcgtc ctggatcagg gtgagtgcgt cgttcatgcg
      961 gcctcctggg catcgtgcga cgaaccatgc gttcgccgat gatccgtcta cgcgccccga
     1021 cgcgggtcgg attcaggcgc ggccccccac gttcagagcg ccagggccat gtagcggatg
     1081 tgcgaccgga acacctcggg catctccacc ggatacggcg cgcagtcgac gaaccccagc
     1141 gactcgtaga cggcctgggc cgcgcgcatg aacggcagcg tatcgagccg cacggtgcga
     1201 tagccgaagg tgcgcgcgtc gtccagcagc cggcgcgtga gcgcctcgcc cagccggtgg
     1261 ccgcgccccg cgggacgcac gtagacgcgc ttgagctcgg cgaccccgtc ctcgctgcgg
     1321 cgcagtccgc ccatggcgac gacgccgccg gcgtgctcga cgaggtagaa cgcgccgcgt
     1381 ggcggcgggt cgccgcagac cttgtcgatg acggtcggca catagtcgga cacctcggcg
     1441 ccgaggatcc cgcgcgcggt catccccgac atggcctcga tctcggcgaa cacccaggcg
     1501 acgtactcga tgttgatggc caggagggtg gcgcggtcgg cgatggggtc ggcgaggcgg
     1561 aaggtcggcg cactcatggc tggggtcagg tcttgaacgt tgcggggtca ggtcttgagt
     1621 tttgcacggg agcgggtggc agggctgcgg cgaagatctc cggatcgccg gcccgctgtg
     1681 atggctcttc cacgtcttgc tctatggact gcacgtttca agacctgacc ccgcaacgtt
     1741 caagacctga ccccaccctc gcgcgcaccc tcgctcgagt tcgccggttc cgccgaagcg
     1801 tcatcctgcg cgcagtcgca ggatccacgg tggcgcgcca ggccgcatcc cgaggtgcga
     1861 aaatcggccc atgaccacgc tcaacgaact ttcgatccag acgcccgacg actggcacct
     1921 gcacatccgt gacggcgccg cgatggcggc cgtggtgccg tacaccgcgc gccagttcgg
     1981 ccgcgcgatc atcatgccca acctgcgtcc gccgatcacg acgaccgcgc aggccgtggc
     2041 ctaccgtgag cgcatcgtgg ccgcgctgcc cgagggcagc acgttcgagc cgctgatgac
     2101 gctgtacctg accgacaacc tgccgcccga cgagatcaag cgtgcaaagg acgccggcat
     2161 cgtcgcggtg aagctgtacc ccgccggcgc gacgacgaac tcggacgccg gcgtgaccga
     2221 cctgcgcaag acctacaaga cgctcgaggc gatgcagcgc gagggcatca agctgctggt
     2281 gcacggcgag gtgacgagtg ccgagatcga cctgttcgac cgcgaggccg tgttcatcga
     2341 ccaggtgttc gagccgctgc ggcgcgacct gcccgagctg aaagtggtgt tcgagcacat
     2401 cacgaccagg gaggccgcgc agtacgtggc cgagtcgagc gccttcaccg cggcgacgat
     2461 caccgcccac cacctgctgt acaaccgcaa cgccatcttc ctgggcggcc tgcgcccgca
     2521 ctactactgc ctgcccgtgc tcaagcgcga ggtgcaccgc caggcgctgg tggccgcggc
     2581 cacgtcgggc agcgcgaagt tcttcctggg caccgacagc gcgccgcacg cgtcggtgat
     2641 gaaggaagcg tccgtgtgcg gcgccggctg cttcacggcg ctgtcggcgc tcgagctcta
     2701 tgccgaagcc ttcgacggcg tcggcgcgct cgacaagctc gagggcttcg ccagccacca
     2761 cggccccgac ttctacggcc tgccgcgcaa caccggcacc gtgacgctca ggcgcgagtc
     2821 ctgggaactg ccggcgagcg tgccgttcgg cgaggccgcc gtcctcaagc cgctgcgcgc
     2881 cggcgagacc ctcggctgga agcaggcctg accgccccgc gcgtcacgtt cgagcccgcg
     2941 gtcgccgccg acgccgaggc gctggtggcg atccgcatcg ccgcgatgcg cgagagcctg
     3001 gagcgcctcg gccgcttcga cccgcagcgc gcccgcgacc gcttcctcgc cgccttcgag
     3061 ccggcgctga cgcgccacgt gctggccggc ggcgagcgcg tcggcttcgt cgtcgtgcgc
     3121 ccccaggacg acggcctgct gctcgaccac ctctacctgt tgccgactgc ccagggccgc
     3181 ggcgtcggcg ccgccgtgct ggccgacgtc ctcgcgcgcg cggacgcgtc acgcctgccc
     3241 gtgcgcgtgg gcgcgctgcg cgggagcgac tcgaaccgct tctaccagcg ccacggcttc
     3301 gaactggtcg agcagggcga gtgggacaac ctgtacctcc gtccggtgcg ctgaacgtcc
     3361 gtgccgcgtc ccaacggccg agcgcggcgt ggggacacct cgcatcgacc gggagcgccg
     3421 gggcggccca tgggacaatc caccccgtga agcgagccag accgccgctg gcacaaccgc
     3481 cgaaaggccg tgctggagga aggtccggac tgcacagggc aacgcagcag ctaacggctg
     3541 tccgccgagt gccgcaaggc atgaaggcga ggatcagagc aacagagacg agccgattca
     3601 gttcgggtga aacgggcaat ctctgcgtgc agcaacatca agtaggcgga cgtcgacctg
     3661 gcccggggag tccgcgggta gatggcatcg agccgtgctg gcgacagccg gcccagacga
     3721 atggcggtca cggcgcgcgc gcccgcaagg gaacgcgcga cgcacagaat ccggcctatc
     3781 ggcccgcttc acattaattc caggcggcac gcgcgcggcg cgggccgtca acgagccgac
     3841 acctgcatga atcctgacgc cgcaaccctc tggcgccgac cgcggtcggc gctggcggcg
     3901 ctcctcgcat gcctgggcat gctgggcgcg ttctcgatcg acacctacat cccggcgttc
     3961 gccggcatcg cgcgcgcgct cgacgccacg ccggtgcaga tgcagcagac gctgtcggcc
     4021 tatctgttcg gcttcgcggt gatgaacctg ttccacggcg cactggccga cagtttcggg
     4081 cgccggccgg tcgtgctgtg gggcatcgcc gtgttcacgc tgtcctcgct cggctgcgcg
     4141 ctgtcgcagt ccatcgggca cctcgtgttc ttccggctcg cgcagggcat gagctcgggc
     4201 gcgagcatgg tggtgggccg cgcgatcatc cgcgacatgt tcccgcccga cgaggcgcag
     4261 cgggtgatgt cgcaggtcac catctacttc ggcgtggcgc cggcggtcgc gcccatcgtc
     4321 ggcggcctgc tgctggtgca ctacggctgg cactcgatct tcatcttcct gaccggcatc
     4381 ggcgtgctgc tgtggtcgct cagctggcgc ctgctgcccg agacgctgca ccacacgcag
     4441 gtgcagccct tccacccggc tcacctgctc aagggctact gggggatgtt cacgagtccg
     4501 cgcttccttg cgctggcgct ggcctcgggc gtgccgttca acgggatgtt cctgtacatc
     4561 ctgtcggcac cggtgttcct gggcgacctg ctgcacctgg acccgacgca cttcttcttc
     4621 ctgttcctgt tcaccatcgg cgggatcatg ggcggagcct tcctgtcggg gaagctcgcc
     4681 ggcaagatga agcccgcgcg ccagatccgc atcgggttca cgatcatgtt ctcggtcgcg
     4741 gtgctcaacc tggtgctgaa ctcgctgttc acggcgcacg cgtggtgggc gatcccgccg
     4801 atcggcgtgt tctcgttcgg ctgggcgctg atggtgccgg tggtgacgct gctggtgctg
     4861 gaccaggcgc cgctgcggcg tggcatggcc tcgtcgctcc aggcctgcat cgggtcggcc
     4921 gccaacggcc tggtcgcggg tctgctggtg ccgatggtga tgcactccac cgtggctctg
     4981 gcgtgcgcgt cggccgcgtt gatgctggtc gggctggtgg cctggtacgg cgtgcgg
//
LOCUS       DATCRO010000514         9515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_118138, whole genome shotgun sequence.
ACCESSION   DATCRO010000514 DATCRO010000000
VERSION     DATCRO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118138"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VIN75_14075"
     CDS             <1..183
                     /locus_tag="VIN75_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEY8975345.1"
                     /translation="MMSIGMHCRLLGRPGRLRALQRFLDHVQRHDRVWITRRVDIARH
                     WKQVHPFDPDRAFTWE"
     gene            186..1967
                     /gene="uraD"
                     /locus_tag="VIN75_14080"
     CDS             186..1967
                     /gene="uraD"
                     /locus_tag="VIN75_14080"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY8975346.1"
                     /translation="MSTLTLERLNAASRAEFVALLDGTYEHSPWIADAAFDARPFASL
                     PELKQALVRAVRVAGREAQLGLIRAHPELAGKAMVARTLTAESTSEQGKAGLTACTPE
                     EFARIGELNAAYNARFGFPFILAVRGPRGTGLTRHQIIAAFERRAANHPDFEFAEALR
                     NIDRIAEIRLDDKFGTSPVQGNLVWDWAESLARFSEPGYAERGELTVTYLTDAHRAVA
                     AQLADWMRTDCGFDEVTIDAVGNVVGVYHGSDRAAPRLVTGSHYDTVRNGGKYDGRLG
                     ILVPMACVRELHRRGERLPHGFEVIGFAEEEGQRYDAVFLGSGALTGHFDPKWLDQAD
                     ANGVRMRDAMAAAGLHPEDIPALKRDPARYLGFVEVHIEQGPVLNELDLPLGVVTSIN
                     GSVRHVGEIIGLASHAGTTPMDRRRDAAAGVAELVLFVERRARAEPHLVGTVGILEVP
                     NGSINVVPGRCKFSLDVRAPSDAVRDACSRDILAELAALCERRGLSFRLHETMRAAAA
                     PSAPEWQRRWEHAVESLGLPVHRLPSGAGHDAMKMHTLLPQAMLFVRGENAGISHNPL
                     ESTSSHDIDLAVQAFARLLAHLASETR"
     gene            1964..3199
                     /locus_tag="VIN75_14085"
     CDS             1964..3199
                     /locus_tag="VIN75_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8975347.1"
                     /translation="MTSSAYDQLDRWIDTHFDEQVRLLQELVRVPTDTPPGNNTPHAL
                     RTQELLREFDFAAQAHEVPAATVREHGLQSITNLVVQRRYGSGGRVVALNAHGDVVPP
                     GEGWTHDPYGGEVVDGKLYGRAAAVSKCDFTTFTFAVRALEALGAPLAGGVDLLFTYD
                     EEFGGELGPAWLLERGLTKPDLEIAAGFSYQVVTAHNGCLQLEITVHGKMGHAAVPDT
                     AVDALQAANRILTALYAENAVLAKTRSGVRGINHGYVNVGQIAGGTNTNVVPGKVVLK
                     VDRRMIPEEDPVAVEAGLRALIERAAAGQAGISVDVRRILLAHAMKPLAGNAPLVDAL
                     CSHATALFGETVEAMGTPLYTDARLFSERGIPAVIYGAGPRTVLESNAKRADEHIVLE
                     DLRRATKVVARTLFDLLKA"
     gene            complement(3363..4295)
                     /locus_tag="VIN75_14090"
     CDS             complement(3363..4295)
                     /locus_tag="VIN75_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8975348.1"
                     /translation="MDRLSAVKLFMRVVELGSFSKAATELGIGQPAVTKQVQRMEEQL
                     GARLLHRSTHGVTATEIGALYYEKCRTIAHHVEEADSVAALLQSQVRGAMRVSTSVAF
                     GRRVLGPLLMQFMRANPDLQIDLGVDDRYVDMIEQGVDVAVRMGRLADSTLGARYLGM
                     NPWVLVASPAFLRERDAPAAPQDLAARDCLIYSTVQGDARWHFTGAGGQAESVAVKGP
                     LRSNSLSLLLEAAVEGMGIAALPRYVAQQALVAGAVVPLLGEWSLPAQEVHAVFPSPR
                     MLPLKVSGFVGWLQAQFEGEWWNRTWKAEAERKP"
     gene            4419..6215
                     /gene="gcl"
                     /locus_tag="VIN75_14095"
     CDS             4419..6215
                     /gene="gcl"
                     /locus_tag="VIN75_14095"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEY8975349.1"
                     /translation="MARMKAAMAAVLVMEREGVTQAFGVPGAAINPLYAQMRERQSIA
                     HILARHVEGASHMAEGYTRARAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPAQVPRVFQQAFHLMRSGRPGPVLI
                     DLPFDVQMAEIEFDIDSYEPLPVYKPAATRKQVEKALDMLAAADKPLIVAGGGIINAD
                     ASALMQEFAEITGVPVIPTLMGWGTIPDDHPLMAGMCGLQTSHRYGNATLLASDFVLG
                     IGNRWANRHTGSIDVYTKGRKFVHVDIEPTQIGRVFNPDLGIASDAGAALELFVEVAR
                     ERASAGRLKDYGDWAFRCRERKRLMHRKSHYTQVPIKPMRVYEEMNAAFPRDTIYVST
                     IGLSQIAGAQFINVHGPRQWINCGQAGPLGWTIPAALGVVAADPTRTVVGLSGDYDFQ
                     FLVEELAVGAQFRLPYVQVLVNNSYLGLIRQSQRNFDMDFCVQLAFENQNVPAGEGDL
                     RGYGVDHQQVVAGLGCKSLRVTDPEQIRSALVQAQAMAREFRVPVVVEVILEKVTNIA
                     MGTEIDNIAEFEEIDCRHPQGRTGLELAGLLD"
     gene            6238..7017
                     /gene="hyi"
                     /locus_tag="VIN75_14100"
     CDS             6238..7017
                     /gene="hyi"
                     /locus_tag="VIN75_14100"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEY8975350.1"
                     /translation="MPRFAANLTMLFTEFPFLDRFERAARAGFKGVEFLFPYPFQPKD
                     IRARLEQFDLRLVLHNLPAGNWDAGERGIACHPDRCAEFREGVERAIDYAGALGVSQL
                     NCLVGKTPADVPAAQVHETLVDNLWRASQRLKEAGIRLLVEPINRFDIPGFHLHRTDQ
                     ALALIDEIGGDNVFLQYDIYHAQRTEGELAETLRKHLSRIAHVQVADNPGRNEPGTGE
                     INFPFLFGHLDRIGYQGWIGCEYKPAVSTEIGLEWIQRIAK"
     gene            7050..7952
                     /gene="glxR"
                     /locus_tag="VIN75_14105"
     CDS             7050..7952
                     /gene="glxR"
                     /locus_tag="VIN75_14105"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEY8975351.1"
                     /translation="MTNASPKIGFIGLGIMGAPMAGHLIAAGHQLFVHTKGKMPETIT
                     ETRATQCVDARGVAERADIVFIMVPDTPDVEAVLFGDGGVAAGLSRGKIVVDMSSISP
                     IATKRFARRVNDIGCDYLDAPVSGGEVGARNATLSIMVGGDEGVFARVKPLFELMGKN
                     ITLVGGNGDGQTAKVANQIIVALNIEAVAEALLFAARAGADPAKVRQALMGGFAASRI
                     LEVHGERMIKRTFDPGFRIELHQKDLNLALASARALGVSLPNTANAQELFNACAAHGG
                     KAWDHSGMVRALEKLANFEIGQAA"
     gene            8060..9365
                     /locus_tag="VIN75_14110"
                     /pseudo
     CDS             8060..9365
                     /locus_tag="VIN75_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
BASE COUNT         1475 a         3501 c         3262 g         1277 t
ORIGIN      
        1 atgatgagca tcggcatgca ctgccgcctg ctcggacgcc ccggccgcct gcgcgcgctg
       61 cagcgcttcc tcgaccacgt gcagcgccac gaccgcgtgt ggatcacccg ccgcgtcgac
      121 atcgcgcggc actggaagca ggtgcatccc ttcgatccgg accgggcctt cacctgggaa
      181 tgagcatgtc caccctcacc ctcgagcggc tcaacgccgc atcgcgcgcg gagttcgtcg
      241 cgctcctgga cggcacttac gagcattcgc cgtggatcgc cgacgccgcg ttcgacgcgc
      301 gcccgttcgc gagcctgccg gagctcaagc aggccctcgt gcgcgcggtg cgcgtggccg
      361 ggcgcgaggc gcagctcggg ctgatccgcg cccacccgga actcgccggc aaggcgatgg
      421 tcgccaggac gctcaccgcc gagagcacca gcgagcaggg caaggcgggc ctcacggcct
      481 gcacgcccga ggagttcgcg cgcatcggcg agctcaacgc ggcctacaac gccaggttcg
      541 gcttcccgtt catcctcgcc gtgcgcggcc cgcgcggcac gggcctgacc cgccaccaga
      601 tcatcgccgc cttcgagcgc cgcgccgcga accacccgga cttcgagttc gccgaggcgc
      661 tgcgcaacat cgaccgcatc gcggagatcc gcctcgacga caagttcggc acgtcgcccg
      721 tgcagggcaa cctcgtgtgg gactgggccg agtcgctcgc gcgcttcagc gagcccggct
      781 atgccgagcg cggcgagctg acggtcacct acctgaccga tgcgcaccgc gccgtcgccg
      841 cgcagctggc cgactggatg cgcaccgact gcggcttcga cgaggtcacg atcgacgcgg
      901 tcggcaacgt cgtcggcgtc taccacggca gcgaccgcgc ggcgccgcgg ctcgtcaccg
      961 gcagccatta cgacacggtg cgcaacggcg gcaagtacga cggccgcctg ggcatcctgg
     1021 tgccgatggc ctgcgtgcgc gaattgcacc ggcgcggcga gcgcctgccc cacggcttcg
     1081 aggtgatcgg cttcgccgag gaggaaggcc agcgctatga cgcggtcttc ctcggctcgg
     1141 gcgcgctcac cgggcacttc gacccgaagt ggctggacca ggccgacgcg aacggcgtgc
     1201 gcatgcgcga cgcgatggcc gcggccggac tgcaccccga agacatcccc gcgttgaagc
     1261 gtgacccggc gcgctatctc ggcttcgtcg aagtgcacat cgagcagggc cccgtgctca
     1321 acgagctcga cctgccgctg ggcgtggtca cgtccatcaa cggcagcgtg cggcacgtcg
     1381 gcgagatcat cggactcgcg agccacgccg gcaccacgcc gatggaccgc cgccgcgacg
     1441 ccgccgccgg cgtcgccgag ctggtgctgt tcgtcgagcg ccgcgcgcgc gccgagccgc
     1501 acctggtcgg cacggtcggg atcctggagg tgcccaacgg ctcgatcaac gtggtgcccg
     1561 gccgctgcaa gttcagcctc gacgtgcgcg cgccgagcga cgccgtgcgc gacgcctgct
     1621 cgcgcgacat cctggccgaa ttggccgcgc tgtgcgaacg ccgcggcctg tcgttccggc
     1681 tgcacgagac catgcgcgcc gccgccgcgc ccagcgcgcc cgaatggcaa cggcgttggg
     1741 aacacgccgt cgagtcgctg ggcctgcccg tgcaccgcct gcccagcggc gccggccacg
     1801 acgccatgaa gatgcacacg ctgctgccgc aggccatgct gttcgtgcgc ggcgagaacg
     1861 ccggcatcag ccacaacccg ctggagtcga cctccagcca cgacatcgac ctcgcggtgc
     1921 aggccttcgc ccgcctgctc gcccacctcg cctcggagac ccgatgactt caagcgccta
     1981 cgaccagctc gatcgctgga tcgacaccca cttcgacgag caggtgcgcc tgctgcagga
     2041 actcgtgcgc gtgcccaccg acacgccgcc cggcaacaac acgccgcacg cgctgcgcac
     2101 gcaggaactg ctgcgcgagt tcgacttcgc cgcgcaggcg cacgaggtgc ccgccgcgac
     2161 cgtgcgcgag cacggcctgc agtccatcac caacctggtc gtgcagcgcc gctacggcag
     2221 cggcggccgc gtcgtcgcgc tcaacgcgca cggcgacgtc gttccgcccg gcgaaggctg
     2281 gacgcacgac ccgtacggcg gcgaagtcgt cgacggcaag ctctacgggc gcgccgccgc
     2341 ggtcagcaag tgcgacttca cgacgttcac gttcgcggtg cgcgcgctcg aggcgctcgg
     2401 cgcgccgctg gccggcggcg tcgacctgct gttcacctac gacgaggaat tcggcggcga
     2461 actcggcccg gcgtggctgc tggaacgcgg cctgacgaag cccgacctcg agattgccgc
     2521 cggcttcagc taccaggtcg tcaccgcgca caacggctgc ctgcagctgg agatcaccgt
     2581 gcacggcaag atgggccacg ccgcggtgcc ggacaccgcc gtcgacgcgc tgcaggccgc
     2641 caaccgcatc ctgaccgcgt tgtacgcgga gaacgcggtg ctcgcgaaga cgcgcagcgg
     2701 cgtgcgcggc atcaatcacg gctacgtcaa cgtcggccag atcgccggcg gcaccaacac
     2761 caacgtggtg cccggcaagg tggtgctcaa ggtcgaccgc cgcatgatcc ccgaggagga
     2821 tccggtggcc gtcgaggccg gcctgcgcgc gctgatcgag cgcgcggccg cgggccaggc
     2881 gggcatcagc gtcgacgtca ggcgcatcct gctcgcccac gcgatgaagc ccctggccgg
     2941 caacgcgccg ctggtcgacg cgctgtgctc ccacgccacg gccctgttcg gcgagacggt
     3001 ggaggccatg ggcacgccgc tgtacaccga cgcacgcctg ttcagcgagc gcggcatccc
     3061 cgcggtgatc tacggcgcag ggccgcgcac ggtgctggag tccaacgcga agcgggccga
     3121 cgagcacatc gtgctggagg acctgcggcg cgcgaccaag gtcgtcgcgc gcacgctgtt
     3181 cgacctgctc aaggcatagc ggcgcgacac cgtccgcagc caccgccgcg tcgtcacctg
     3241 cgcgcagtcg caggatccac ggcggcgtac cgatgcgcgt catggcgggc agaggtggat
     3301 cctgccactg cgcaggatga cgggtcacgg atggcgccct cattggcgcc tcggaggaca
     3361 agctacggct tgcgttcggc ttcggccttc cacgtccggt tccaccactc gccctcgaac
     3421 tgcgcctgca gccagcccac gaagcccgac accttcaacg gcagcatgcg cggcgagggg
     3481 aacaccgcgt gcacctcctg cgcgggcagc gaccactcgc cgagcagcgg gaccaccgcg
     3541 cccgcgacca gcgcctgctg cgcgacgtag cgcggcagcg ccgcgatgcc catgccctcg
     3601 acggccgcct ccagcagcag tgacaggctg ttggagcgca gcggtccctt caccgccacc
     3661 gactccgcct gcccgcccgc gccggtgaag tgccagcgcg cgtcgccctg cacggtgctg
     3721 tagatcaggc agtcgcgcgc cgcgagatcc tggggcgccg ccggcgcgtc gcgttcgcgc
     3781 aggaacgccg gcgaggccac cagcacccag gggttcatgc cgagatagcg ggcgcccagc
     3841 gtcgagtcgg ccagccggcc catgcgcacc gcgacgtcga cgccctgctc gatcatgtcg
     3901 acgtagcggt cgtccacgcc caggtcgatc tgcaggtcgg ggttggcgcg catgaactgc
     3961 atcagcagcg gccccagcac gcggcggccg aacgcgaccg aggtgctcac gcgcatcgcg
     4021 ccgcgcacct gcgactgcag cagcgcagcg accgaatcgg cctcctcgac gtggtgtgcg
     4081 atggtgcggc acttctcgta gtacagcgcg ccgatctcgg tggccgtgac gccgtgcgtg
     4141 gagcgatgca gcaggcgcgc gccgagctgc tcctccatgc gctggacctg cttggtgacc
     4201 gcgggctggc cgatgcccag ctccgtcgcg gccttgctga acgaaccgag ctcgacgacg
     4261 cgcatgaaga gcttgacggc ggagagacgg tccatggcgc ggtgttcgac gggtattcca
     4321 ttgtggaata aacatcatgc ttgtcaacgg cgttcctcga tgcgccgcgc tcgccgacca
     4381 tcacgccaac ccccaagcaa gcagtgatgg agacgaacat ggcaaggatg aaggccgcga
     4441 tggcggcagt gctggtgatg gaacgcgaag gcgtgacgca ggcgttcggc gtgccgggcg
     4501 cggcgatcaa tccgctgtac gcgcagatgc gcgagcggca gtcgatcgcg cacatcctgg
     4561 cgcgccacgt ggaaggcgcg tcgcacatgg ccgagggcta cacgcgcgcc cgtgccggca
     4621 acatcggcgt gtgcatcggc accagcggcc ccgcgggcac cgacatgatc acgggcctgt
     4681 attcggccag cgccgactcg atccccatcc tgtgcatcac cggccaggcg ccgcgcgccc
     4741 gcctgtacaa ggaagacttc caggccgtgg acatcgagtc catcgccaag cccgtcacca
     4801 agtgggccgt caccgtgcgc gagcccgcgc aggtgccgcg cgtgttccag caggccttcc
     4861 atctgatgcg ctcgggccgc ccgggcccgg tgctgatcga cctgccgttc gacgtgcaga
     4921 tggccgagat cgagttcgac atcgacagct acgagccgct gcccgtctac aagcccgcgg
     4981 ccacgcgcaa gcaggtcgag aaggcgctgg acatgctggc cgccgccgac aagccgctga
     5041 tcgtcgccgg cggcggcatc atcaacgcgg acgccagcgc gctgatgcag gagttcgccg
     5101 agatcaccgg cgtgcccgtc atccccacgc tgatgggctg gggaaccatc cccgacgacc
     5161 acccgctgat ggccggcatg tgcggcctgc agaccagcca ccgctacggc aacgccacgc
     5221 tgctggccag cgacttcgtg ctgggcatcg gcaaccgctg ggccaatcgc cacacgggct
     5281 cgatcgacgt ctacaccaag ggccgcaagt tcgtgcacgt cgacatcgag cccacgcaga
     5341 tcggccgcgt gttcaacccc gacctgggca tcgccagcga cgccggcgcg gcgctcgagc
     5401 tgttcgtcga ggtcgcccgg gagcgcgcgt ccgcgggccg gctgaaggac tacggcgact
     5461 gggcgttcag gtgccgggag cgcaagcgcc tgatgcaccg caagagccac tacacgcagg
     5521 tgccgatcaa gccgatgcgc gtatacgagg agatgaacgc cgcgttcccg cgcgacacga
     5581 tctacgtctc cacgatcggc ctgagccaga tcgccggcgc gcagttcatc aacgtccacg
     5641 gcccgcgcca gtggatcaac tgcggccagg ccggcccgct gggctggacg atccccgcag
     5701 cgctgggcgt ggttgccgcc gatccgacgc gcaccgtcgt cggcctgtcg ggcgactacg
     5761 acttccagtt cctcgtcgag gagctcgccg tcggcgcgca gttccgcctg ccctacgtgc
     5821 aggtgctcgt gaacaacagc tacctcggcc tgatccgcca gtcgcagcgc aacttcgaca
     5881 tggacttctg cgtgcagctc gcgttcgaga accagaacgt gcccgcgggc gaaggcgacc
     5941 tgcgcggcta tggcgtcgac caccagcagg tcgtcgccgg cctgggctgc aagtcgctgc
     6001 gcgtgaccga ccccgagcag atccggtcgg cgctcgtgca ggcgcaggcg atggcgcgcg
     6061 agttccgcgt gccggtggtc gtcgaggtga tcctggagaa ggtcaccaac atcgcgatgg
     6121 gcaccgagat cgacaacatc gcggagttcg aggaaatcga ctgccgccat ccgcagggac
     6181 gcacgggcct ggagctcgcc ggcctcctcg attgaacaca ccacgactgg acacacgatg
     6241 cctcgcttcg ccgccaacct cacgatgctc ttcacggagt tcccgttcct cgaccgcttc
     6301 gagcgcgccg cgcgcgccgg cttcaagggc gtggaattcc tgttccccta cccgttccag
     6361 cccaaggaca tccgcgcgcg gctcgagcag ttcgacctgc ggctggtgct gcacaacctg
     6421 cccgccggca actgggacgc gggcgagcgc ggcatcgcct gccatcccga tcgctgcgcg
     6481 gagttccgcg agggcgtcga gcgcgcgatc gactacgccg gcgcgctggg cgtcagccag
     6541 ctcaactgcc tggtcggcaa gacgccggcc gacgtgcccg ccgcccaggt gcacgagacg
     6601 ctggtggaca acctctggcg cgcgtcgcag cggctgaagg aggccggcat caggctgctc
     6661 gtcgagccga tcaaccggtt cgacatcccc ggcttccacc tgcaccgcac cgaccaggcg
     6721 ttggcgctga tcgacgagat cggcggcgac aacgtgttcc tgcagtacga catctaccac
     6781 gcgcaacgca ccgaaggcga acttgccgag acgctgcgca agcacctgtc gcgcatcgcg
     6841 cacgtgcagg tcgccgacaa cccgggccgc aacgagccgg gcaccggcga gatcaatttc
     6901 ccgttcctgt tcggccacct cgaccgcatc ggctaccagg ggtggatcgg ctgcgaatac
     6961 aagcctgcgg tctccaccga gatcggcctg gagtggatcc agcgcatcgc caagtgagcc
     7021 gcaccttcat ttcaagcaga gagatcgaga tgaccaacgc atccccgaag atcggcttca
     7081 tcggcctggg catcatgggc gcgccgatgg cgggccacct catcgccgcc ggccaccagc
     7141 tgttcgtgca caccaagggc aagatgcccg agaccatcac cgagacgcgc gccacgcagt
     7201 gcgtcgacgc gcgcggcgtg gccgaacggg ccgacatcgt cttcatcatg gtgcccgaca
     7261 cgcccgacgt cgaggccgtg ctgttcggcg acggcggcgt ggccgccggg ctgtccaggg
     7321 gcaagatcgt cgtcgacatg agctccatct cgccgatcgc caccaagcgc ttcgcgcgcc
     7381 gcgtcaacga catcggctgc gactacctgg acgcgccggt ctcgggcggc gaggtgggcg
     7441 cgcgcaacgc gacgttgtcc atcatggtgg gtggcgacga aggcgtgttc gcgcgcgtca
     7501 agccgctgtt cgagctgatg ggcaagaaca tcacgctggt gggcggcaac ggcgacgggc
     7561 agacggccaa ggtggccaac cagatcatcg tggcgctcaa catcgaggcc gtcgccgagg
     7621 cgctgctgtt cgccgcgcgc gccggcgccg acccggccaa ggtgcgccag gcgctgatgg
     7681 gcggcttcgc cgccagccgc atcctggagg tgcacggcga gcgcatgatc aagcgcacgt
     7741 tcgacccggg cttccgcatc gagctgcacc agaaggacct gaacctcgcg ctggccagcg
     7801 cgcgggcgct gggcgtctcg ctgcccaaca cggccaacgc gcaggagctg ttcaacgcgt
     7861 gcgcggccca cggcggcaag gcctgggatc actcgggcat ggtgcgcgcg ctggagaagc
     7921 tcgcgaactt cgagatcggg caggcggcgt gagcgccgcc cggccgcccg aaggcgttcc
     7981 ctcctgcgtc ggtcacaccg cagtgcgcag cacgcaggcc tgtccggctg cgacggccga
     8041 gaccacgctg ctgcgccgca tgttcgccgc cgcggtggcc agcgcgcagc ccgcgctgtg
     8101 cgtgccgccg cacctgccgc ggcccgagga gatcggtgac ggtcgcctcg tggtcatcgg
     8161 cgccggcaag gcctcggccg cgatggcgca ggcggtcgag gcgaactggc acgggccgct
     8221 gtccggcctc gtcgtcacgc gctacggcta cggcgcgccg tgcgaccgca tcgagatcgt
     8281 cgaggccgcg catccggtgc ccgacgaggc cggcctgcgc gccgcccagc gcatgctgga
     8341 cgtcgtgcgc gacctgcggc cgcaggacgt cgtgctgtgc ctgatctcgg gcggcggctc
     8401 ggcgctgctg ccgctgccgc tgccgggcct cacgctggaa gacaagcagg ccgtcaaccg
     8461 cgcgatgctc gccagcggcg cgaccatcgg cgagatgaac tgcgtgcggc gccacctgtc
     8521 ggccatcaag ggcggacgcc tcgcggcggc ctgccatccg gcgcgcgtca tcacgctgct
     8581 gatgtcggac gtgccgggcg accggcctct ggacatcgca tcgggcccga cggtcgccga
     8641 cccgacgacg tgccacgacg cgctcgacat cgtgcgtcgc cacgagctcg cgctgcctgc
     8701 cgccgcgatg gcgctgctcg agagcgggcg cggcgaatcg atcaagcccg gcgacccgcg
     8761 gctcgcgcgc gccgatctgc gcatgatcgc gacgccgcag gccgcgctcg aggccgcggc
     8821 cgccgtggcc gcgcaggcgg gttatgccgc gcacatcctc ggcgacgcga tcgagggcga
     8881 ggcgcgcgac gtcgccaagg tgatgggcgg catcgcgctg caggccgcga cgcggcgcca
     8941 gccgttcgcg gcgccgtgcg tgctgctgtc cggcggcgag acgacggtca ccgtgcgcgg
     9001 ccagggccgc ggcgggcgca acgtggagtt cctgctgtcg ctcgcgctcg cgctcgacca
     9061 gcagcccggc gtgttcgcgc tggccggcga caccgatggc gtcgacggcc aggaggagat
     9121 cgccggcgcc gtgctgcgcc ccgacacgct cgcacgggcc cgcgcgctcg ggctgcgcgc
     9181 gcgcgaccgg ctggcggaca acgatgcgca cacgttcttc gagcgcctgg gcgcgcggcc
     9241 tgcgcccgcg cgacgcgctg gacgacaacg acgcccacac gttcttcgag acgctcggcg
     9301 acgccgtgat caccggcccc acgcggacga acgtgaacga tttccgggcg gtgctgatcg
     9361 cctgatttcc ggcgcgacga gaccggaggg tccggcctct ccgaacgccg cgcaaggaca
     9421 tcgagcagcg aggtgatgcg actcgcggag ggaggagagg ccagacccct tcgaccgcct
     9481 tgcctccagg ccacctcctc gtcaaccctc gacgt
//
LOCUS       DATCRO010000515         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_387747, whole genome shotgun sequence.
ACCESSION   DATCRO010000515 DATCRO010000000
VERSION     DATCRO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387747"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VIN75_14115"
     CDS             complement(<1..1032)
                     /locus_tag="VIN75_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8975352.1"
                     /translation="MPNRPYLNRLLIAFACLWTLQAHAQSFRVQCPASTALHPLVNAL
                     GQTGKPDSNLPNPHIKCQQLGGGDGFATMADGTQTYMFSFGPLSGLSNIVHGLPGTVT
                     AAEFNQSNIDPSGNPIADVRIGAPDGLGYTFNGAIGLAPDINAEQVGAVSFNIDPTTD
                     LPITGTRYKVNDIVVFSGGGGSGATGVVSSVTGTGAINAITVTNPGQDYTSVPDVTIV
                     PFDGRALAGTGAQPVASLMMAVNAMDGHVDPRLVMDVGVMNGNIPAPMISIEEGDELF
                     LTLTNVGMVMRPDLFEQHTVHFHGYPNASSFYDGVPDASVAINIGASFTYYYLAPEAG
                     TYMWHCHISP"
     gene            complement(1090..1785)
                     /locus_tag="VIN75_14120"
     CDS             complement(1090..1785)
                     /locus_tag="VIN75_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8975353.1"
                     /translation="MSRTRLPIDARRSRAAERVASDKGASVVARLALFLAGVLVLLAA
                     ILGKPAQAAAGAEQSAHHHHMAPETQRSLADYAVPRIALVREDGANVVLSDEIDDGRP
                     VVLSFVYTTCTTICPVTSATLAELQSRLGETRDRVHLVSISIDPEQDTPARLREYARR
                     FGAGPEWHHYTGTVGASVASQRAFGVYRGDKMSHAPVTLVRAGPNERWVRFEGFATAD
                     QLLAELHRSTDTR"
     gene            2149..>2559
                     /locus_tag="VIN75_14125"
     CDS             2149..>2559
                     /locus_tag="VIN75_14125"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8975354.1"
                     /translation="MSKQADEDARNGPPTTWGRRAGDRLPPDDRERLDRLAELARHAV
                     AAREEERRRLAAELHDVVGTNLAAIKLQLSALASVLPAGCEARIEDAQRLLAETVAGI
                     RDLCGTLRPATLEYAGFTAALGAHCARFSHRTGIE"
BASE COUNT          375 a          855 c          926 g          403 t
ORIGIN      
        1 cggcgagatg tggcagtgcc acatgtacgt gccggcctcg ggcgccaggt agtagtaggt
       61 gaagctggcg ccgatgttga tcgcgaccga ggcgtccggc acgccgtcgt agaaggacga
      121 cgcgttcggg tagccgtgga agtggacggt gtgctgctcg aacaggtcgg ggcgcatgac
      181 catgcccacg ttcgtcagcg tgaggaacag ctcgtcgccc tcctcgatgg agatcatcgg
      241 cgcagggatg ttgccgttca tgacgcccac gtccatgacc aggcgcgggt cgacatggcc
      301 atccatcgcg ttgaccgcca tcatcagcga cgcgaccggc tgcgcgcccg tgcccgccag
      361 ggcccggccg tcgaacggca cgatcgtgac gtcgggcacc gacgtgtagt cctgccccgg
      421 gttggtgacc gtgatggcgt tgatcgcgcc ggtgccggtg acgctcgaga cgacgcccgt
      481 cgcgccgctg ccgccaccac cggagaagac gacgatgtcg ttgaccttgt agcgggtgcc
      541 ggtgatgggc aggtcggtgg tcggatcgat gttgaagctg acggccccca cctgctccgc
      601 gttgatgtcg ggggcgaggc cgatcgcgcc gttgaaggtg tagcccaggc cgtccggcgc
      661 gccgatgcgg acgtcggcga tcgggttgcc cgacggatcg atgttgctct ggttgaactc
      721 ggcggccgtc acggtgccgg gcaggccgtg cacgatgttg ctcagacccg agagcggccc
      781 gaacgagaac atgtaggtct gcgtgccgtc ggccatcgtc gcgaacccgt cgccgccgcc
      841 cagctgctgg cacttgatgt gcggattggg caggttgctg tccggcttgc cggtctggcc
      901 cagcgcgttg accaggggat gcagcgccgt gctggcgggg cactggacac ggaagctctg
      961 cgcatgggcc tgcaacgtcc acaggcatgc gaatgcgatg agcagacggt tcaggtacgg
     1021 acggtttggc atgtcgaatc ccctgtcgtg tctgtgaggc ggtatggcgt gttccgtggt
     1081 tgcgctcgtc tagcgcgtgt ccgtcgaacg gtgcagctcg gccagcagct ggtcggcggt
     1141 ggcgaagccc tcgaagcgaa cccagcgctc gttcggcccg gcgcgcacga gggtgacggg
     1201 tgcgtggctc atcttgtcgc cgcggtacac gccgaaggcc cgctggctcg cgacgctcgc
     1261 gccgaccgtg cctgtgtagt ggtgccactc gggccccgcg ccgaacctgc gcgcgtattc
     1321 gcgcaggcgg gccggcgtgt cctgctcggg atcgatggag atcgagacga gatgcacgcg
     1381 gtcgcgcgtc tcgcccaggc gggactgcag ctccgccagc gtcgcgctgg tgaccgggca
     1441 gatcgtcgtg caggtggtgt agacgaagct cagcacgacg ggcctgccgt cgtcgatctc
     1501 gtcgctcagg acgacgttcg cgccgtcctc gcgcacgagc gcgatccggg gcaccgcgta
     1561 gtcggccagc gagcgctgcg tctcgggcgc catgtggtgg tggtgcgcgc tctgttcggc
     1621 accggcggcg gcctgcgcgg gcttgccgag gatcgccgcc aggaggacga gcacgcccgc
     1681 caggaacagc gccaggcgag cgacgacgct ggcgcccttg tcggatgcga cacgctcggc
     1741 ggctcgtgat cgccgcgcgt cgatcggaag acgtgtccgg gacatcccct ctctctcctt
     1801 gcttgttgtt gccggatcga aaatgcattt gcacctgatc cgacattgcg gttctaccga
     1861 cgcacaaggg ctcgaaccat cgggcgcgga tgagatggga ctaggagtcc tgagcgcgtc
     1921 cctcggaaaa atccgagcgc ggtcgggagg cacggtcacc ccggcgacga cgtcgggacc
     1981 gccccttgcc cacgcgcgta ctcgtggcgc gacatccgac tcccctggaa gggggggcgg
     2041 gtgtcgacgc ccccggggcg cgcgcgcaac gatggccagg gcttaccctg atcgcgcgcc
     2101 ggacttccct tgctagccaa cgcgagtcat tgctggtgca atccgcacat gagcaagcag
     2161 gccgacgagg acgcacgcaa cgggccgccc accacatggg gccggcgcgc cggcgaccgc
     2221 ctgccgccgg acgaccgcga gcgcctggac cggctcgccg aactggcgcg ccacgccgtg
     2281 gccgcgcgcg aggaggaacg tcgccgcctc gcggccgaac tgcacgacgt cgtcggcacc
     2341 aacctggccg ccatcaagct ccagctcagc gccctggcct ccgtgcttcc ggccggatgc
     2401 gaggcgcgca tcgaggacgc acagcgcctg ctggccgaaa cggtggcggg catccgggac
     2461 ctctgcggca cgctgcgtcc cgccacgctg gaatacgccg ggttcacggc cgccctcggc
     2521 gcgcattgcg cgcgcttcag ccaccgcacg ggcatcgag
//
LOCUS       DATCRO010000516         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_714622, whole genome shotgun sequence.
ACCESSION   DATCRO010000516 DATCRO010000000
VERSION     DATCRO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714622"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VIN75_14130"
     CDS             <1..1269
                     /locus_tag="VIN75_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8975355.1"
                     /translation="DQVLEALECAHHAGVRHGDLKLTNVLITSAGLVKVTDFGLARAE
                     GRTGAQAGIAPEYQSGRLIDHRVDIYAAGAILYRMLVGRDPYSNSLVDTQADALSGTL
                     RLPSTIAEAQRPPIFDAVVSRALRLDSSQRFASAAEFRDALRDATAVRVPEHGTGVVT
                     LDSGKPVSASTTIAPMLGELPETGRRTAPSGKAAGAVPTLTIAIPDSVLSMPSHDPWA
                     HNGQPPVPPRQDSDVAPDLVQNDEGYAAALQSQVERTRRASVTAAAAAATAALSAAAD
                     SAASGPAPVPYARRAPPPSVSLPEPMSFKDETPVDERGGIVISRPVSQPMSVRDRMLP
                     DGAAEFDKADLSLPLAGDGVGIPAEALRRTMKVLTVHFGPMAGDVLMHCAPRAGTVPE
                     LHALLVAQAGANIDKKRLAKQLRAVAKMPL"
     gene            complement(1537..1980)
                     /locus_tag="VIN75_14135"
     CDS             complement(1537..1980)
                     /locus_tag="VIN75_14135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY8975356.1"
                     /translation="MPRFPDEPAVVRPPYQKFDVFVRKSEIDGHGVFAAEMIPPRLKI
                     GEIRGESISCAEGRRRAKALQRIMIVEVSSRTAIDASKSTDPMRFTNHSCQANARLAI
                     IAGRIEFYSLRAIEPGEEITVNYGETHHEGKLRCRCGAPNCIGAL"
     gene            complement(1989..2423)
                     /gene="queF"
                     /locus_tag="VIN75_14140"
     CDS             complement(1989..2423)
                     /gene="queF"
                     /locus_tag="VIN75_14140"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY8975357.1"
                     /translation="MPPNPPTAPSKELHVFANPSAGRDYVIQFQIPEFTCHCPLTGQP
                     DFAHFTIDMICDQTCIELKSLKMYMWSYRDEGAFHEKVTNTILDDIVAATDPRFVRIT
                     AKWYVRGGIYTNVVVEHRQKGWTPQPRVDLPAANLGTATGLL"
     gene            2784..>3193
                     /locus_tag="VIN75_14145"
     CDS             2784..>3193
                     /locus_tag="VIN75_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8975358.1"
                     /translation="MRLNSIKLSGFKSFADPTNFQLPGQRVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMMDVIFNGSGTRKPAARASVELVFNNESARAGGPWNQFAEIA
                     VKRVLTREGGSSYFINNQPVRRRDVQDVFLGTGLG"
BASE COUNT          459 a         1130 c         1133 g          471 t
ORIGIN      
        1 gaccaggtgc tcgaagcgct cgagtgcgcc caccacgccg gcgtgcgcca cggcgacctc
       61 aagctcacca acgtgctgat caccagcgcc ggcctggtca aggtcaccga cttcggcctc
      121 gcgcgcgccg agggccgcac cggcgcccag gcgggcatcg cgcccgaata ccagtcgggc
      181 cgcctgatcg accaccgcgt cgacatctac gcggccggcg cgatcctgta tcgcatgctg
      241 gtcggtcgcg atccttacag caattcgctg gtcgacacgc aggccgacgc gctcagcggc
      301 acgctgcgcc tgcccagcac catcgccgag gcccagcgcc cgccgatctt cgacgccgtc
      361 gtctcgcgcg cgctgcgcct ggactcgagc cagcgattcg ccagcgccgc cgaattccgc
      421 gacgcgctgc gcgacgcgac cgcggtgcgg gtgcccgagc acggcacggg cgtcgtgacg
      481 ctggactcgg gcaagcccgt cagcgcctcg accaccatcg ccccgatgct gggcgaactg
      541 cccgagaccg gccgccgcac cgcgcccagc ggcaaggccg ccggcgccgt gcccacgctc
      601 accatcgcca ttcccgacag cgtgctgtcc atgccgtcgc acgacccgtg ggcgcacaac
      661 ggccagccgc cggtgccgcc gcgccaggac agcgacgtcg cgccggatct cgtgcagaac
      721 gacgagggct atgcggccgc gctgcagagc caggtcgagc gcacgcgccg cgcgtcggtc
      781 accgccgccg ccgcggccgc caccgcggcc ctgagcgccg cggccgattc cgcggcctcc
      841 gggccggcgc ccgtgccgta cgcgcgccgc gcgccgccgc ccagcgtctc gctgcccgag
      901 ccgatgtcgt tcaaggacga gacgccggtc gacgagcgcg gcggcatcgt catcagccgt
      961 ccggtgtcgc agccgatgtc cgtgcgcgac cgcatgctgc ccgacggcgc cgccgagttc
     1021 gacaaggccg acctgtcgct gccgctggcc ggcgacggcg tcggcatccc cgccgaggcg
     1081 ctgcgccgca cgatgaaggt gctgaccgtg cacttcggcc cgatggccgg cgacgtgctg
     1141 atgcactgcg ccccgcgcgc gggcaccgtc cccgagctgc acgccctgct ggtcgcccag
     1201 gcgggcgcca acatcgacaa gaagcggctg gccaagcagc tgcgcgcggt cgccaagatg
     1261 ccgctttgag gcggctggcg gtcacggcaa tccgttcgcg gatggacggc cgccaggggt
     1321 ctggcctctc ctcggatccg cgggaggggc ctgcgatcgg caaaggcgtt ggttgcgggt
     1381 ccgaggcgaa ggcctgaccc cgatcagctg acgttcgagg tcaggccttc gcctcatgcc
     1441 gccgcagacg tcctcgcgca tggtcgcatc ccggcggcga catgaggaga ggccagaccc
     1501 ctcgggcccg aagggcggag aggccagact ccttggctac agcgccccga tgcaattggg
     1561 cgccccgcag cggcagcgca gcttgccttc gtggtgcgtc tcgccgtagt tcacggtgat
     1621 ctcctcgccc ggctcgatcg cgcgcagcga gtagaactcg atgcggccgg cgatgatggc
     1681 caggcgcgcg ttggcctggc aggagtggtt cgtgaagcgc atcgggtcgg tcgacttgga
     1741 cgcgtcgatc gccgtgcgcg acgacacctc cacgatcatg atccgctgca gcgccttcgc
     1801 gcggcgccgg ccttcggcgc agctgatgga ctcgccgcgg atctcgccga tcttcaggcg
     1861 cggcgggatc atctcggccg cgaacacgcc gtggccgtcg atctcgctct tgcgcacgaa
     1921 gacgtcgaac ttctggtacg gcggccggac gacggccggc tcatcgggga accggggcat
     1981 ggcggcgttc agagcaggcc ggtggccgtg ccgaggttgg ccgcgggcag gtcgacgcgc
     2041 ggctgcggcg tccagccctt ctggcggtgc tcgacgacga cgttggtgta gatgccgccg
     2101 cgcacgtacc acttggcggt gatgcgcacg aagcgcgggt cggtggccgc gacgatgtcg
     2161 tccaggatgg tgttggtgac cttctcgtgg aacgcgccct cgtcgcggta gctccacatg
     2221 tacatcttca ggctcttcaa ctcgatgcac gtctggtcgc agatcatgtc gatcgtgaag
     2281 tgcgcgaagt cgggctggcc ggtcagcggg cagtggcagg tgaactcggg gatctggaac
     2341 tggatgacgt agtcgcggcc cgcggacggg ttggcgaaca cgtgcagctc cttgctgggc
     2401 gcggtcggcg ggttgggcgg catgtcgcgc gcgccgacct tcagggcgga cttcgaggcc
     2461 ttggcggcct tgggagcgga cttgggagac ggtgcggcag gggtcttctt ggccatgggt
     2521 gcctgcgtcg cgccgggggg gcggcgacgg atgatttcgt ggggggagaa gaccgctgtc
     2581 ccggtcttcg gaggggagga cgcgatggta tcatcccggg ccttttcccg cgtccgcagg
     2641 ggtgctcgcc gtgctccaga cgtcgcttgg aaaatccata taaatcaaca cgttagcgca
     2701 catcaacgcc gaccggcggg agcgcctccc gacgcgcgga ctgacaccgc tccccgacgt
     2761 gccctgcagg cgcaagaccg ttcatgcggc tcaattcgat caagctttcc gggttcaagt
     2821 ccttcgcgga tcccaccaac ttccagctgc ccggccagcg cgtcggcgtc gtcgggccca
     2881 acggctgcgg caagtccaac atcatggacg cggtgcgctg ggtgctgggc gagagcaagg
     2941 cgagcgagct gcgcggcgag tcgatgatgg acgtcatctt caacggctcg ggcacgcgca
     3001 agcccgccgc gcgggcgagc gtcgaactcg tgttcaacaa cgagtcggcg cgcgccggcg
     3061 gcccctggaa ccagttcgcc gagatcgccg tcaagcgcgt gctcacgcgc gagggcggct
     3121 ccagctactt catcaacaac cagccggtgc gccggcgcga cgtgcaggac gtgttcctcg
     3181 gcacgggcct ggg
//
LOCUS       DATCRO010000517         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_163213, whole genome shotgun sequence.
ACCESSION   DATCRO010000517 DATCRO010000000
VERSION     DATCRO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163213"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VIN75_14150"
     CDS             complement(<1..590)
                     /locus_tag="VIN75_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8975359.1"
                     /translation="MRTLLALLAAACVLAGCERTMKDMYVQPKLGPDDASPLFPDGKG
                     SRPPPPGSVAVASGDLAFTSGGRRGRDDLAARDAALAASAPPAAGMALLRRGQSRYDI
                     FCAPCHSALGDGDGRVAQRGFPHPPTFHQARLRQAPDRHFFDVISQGYGEMPSYADRL
                     TPQDRWAVVAYVRALQLSQDARIDTLPADLQAGLRAL"
     gene            complement(587..1120)
                     /locus_tag="VIN75_14155"
     CDS             complement(587..1120)
                     /locus_tag="VIN75_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY8975360.1"
                     /translation="MSDPRWALMAEYETAEALVAAAQAAHDAGFRAIEAYAPFHVEGL
                     AEAVGFERSRLPAVVLAGGLLGGLGGYFLQWYAAVRSYPIEVGARPLHSWPMFVPVTF
                     EMTVLFAAFAAFFGALVANGLPRLAHPIFDAPDFDLATRNRFFLCLRTDDPRFDEARA
                     SAVLDATHPLRRAEVAR"
     gene            complement(1117..2472)
                     /gene="nrfD"
                     /locus_tag="VIN75_14160"
     CDS             complement(1117..2472)
                     /gene="nrfD"
                     /locus_tag="VIN75_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY8975361.1"
                     /translation="MADVPASRESGVLRPGWGYASVTDKIAGLVLTRPVRWPWLAAFL
                     ATMAGTIVYLAAMTYLFIEGVGIWGINIPVAWGFAIGNFVWWIGIGHAGTFISAILLL
                     LRQRWRTSINRFAEAMTLFAAGMAGLFPILHLGRPWFAYWLTPYPDHMNLWPQWRSPL
                     VWDVFAISTYLIVSALFWYQGMLPDLATLRDRAKDRGKRIAYGLMALGWRGEARHWAR
                     HETAYLLLAGIATPLVVSVHSVVAMDFAIGNTPGYHSTIFPPYFVAGALFSGFAMVLT
                     LAIPLRKAFQLEDFITEVHLDHAAKVLLATGWLVAYGYLAENFMAFFSGDRYEIDMTV
                     DRWTGFYAPVYWGLMLCNVVLPQGLWWRKARRSPWLLFALSIVVNLGMWMERFLIVVQ
                     STSHDFMPSAWGRFVPTRWDWIFLFGSISMFAWLFLLFIRLLPAISISEMRELVKEES
                     R"
     gene            complement(2477..>2673)
                     /locus_tag="VIN75_14165"
     CDS             complement(2477..>2673)
                     /locus_tag="VIN75_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975362.1"
                     /translation="GTLRDRAGLRGSRFKQFAYGILALGWRGEARHWARHETAYLLLA
                     ELDTKPRTTYLARIRPADQA"
BASE COUNT          451 a          916 c          981 g          325 t
ORIGIN      
        1 agcgcgcgca agccggcctg caggtcggcc ggcagcgtgt cgatgcgcgc gtcctggctc
       61 agctgcaacg cccggacgta ggcgacgacg gcccagcgat cctgcggcgt caggcggtcg
      121 gcgtaggacg gcatctcgcc gtacccctgg ctgatgacgt cgaagaagtg ccggtcgggc
      181 gcctggcgca gccgcgcctg gtggaacgtc ggcggatggg ggaagccgcg ctgcgcgacg
      241 cggccgtcgc catcgcccag cgcgctgtgg cagggcgcgc agaagatgtc gtagcgcgac
      301 tggccgcgtc gcagcaacgc catgccggcg gcgggtggcg cgctggcggc cagcgcggcg
      361 tcgcgcgcgg cgaggtcgtc gcgcccgcgg cggccgccgc tggtgaacgc gaggtcgccc
      421 gaggccaccg ccacgctgcc cggcggcggc ggccgcgaac ccttgccgtc gggaaacagc
      481 gggctcgcgt cgtccgggcc cagcttgggc tgcacgtaca tgtccttcat cgtgcgttcg
      541 cagccggcga gcacgcaggc cgcggccagc agcgcgagca gcgtcctcat cgcgccacct
      601 ccgcgcggcg cagcggatgc gtcgcgtcga gcacggccga cgcgcgagcc tcgtcgaagc
      661 gcgggtcgtc ggtgcgcagg cacaggaaga agcggttgcg ggtggcgagg tcgaagtcgg
      721 gcgcgtcgaa gatcgggtgc gcgaggcgcg gcagcccgtt ggccacgagc gcgccgaaga
      781 acgcggcgaa ggcggcgaac agcacggtca tctcgaaggt gacgggcacg aacatcggcc
      841 agctgtgaag cggccgggcg ccgacctcga tcgggtacga gcgcaccgcc gcgtaccact
      901 gcaggaagta gccacccagg ccgccgagca ggccgccggc aagcacgacg gccggcagcc
      961 ggctgcgttc gaatccgacc gcctcggcca ggccctcgac gtggaacggc gcataggcct
     1021 cgatcgcgcg gaagcccgcg tcgtgcgcgg cctgcgccgc ggccaccagc gcctccgcgg
     1081 tctcgtactc ggccatcagg gcccagcgag gatcgctcat cggctctcct ccttgacgag
     1141 ctcacgcatc tccgagatgg agatcgccgg cagcagccgg atgaacagca ggaacagcca
     1201 ggcgaacatg ctgatcgagc cgaacaggaa gatccagtcc cagcgcgtcg gcacgaagcg
     1261 gccccaggcc gagggcatga agtcgtggct ggtgctctgc acgacgatca ggaagcgctc
     1321 catccacatg cccaggttca cgacgatgga cagcgcgaac agcagccacg ggctgcgccg
     1381 tgccttgcgc caccacagcc cctgcggcag cacgacgttg cacagcatca gcccccagta
     1441 gaccggcgcg tagaagccgg tccagcggtc gacggtcatg tcgatctcgt agcggtcgcc
     1501 gctgaagaag gccatgaagt tctcggcgag gtagccgtag gcgaccagcc agcccgtggc
     1561 cagcagcacc ttggcggcat ggtccaggtg cacctcggtg atgaagtcct ccagctggaa
     1621 cgccttgcgc agcgggatcg cgagcgtgag caccatcgcg aagccggaga acagcgcacc
     1681 ggccacgaag tagggcggga agatggtgga gtggtagccg ggcgtgttgc cgatggcgaa
     1741 gtccatcgcg accaccgagt gcaccgacac caccagcggc gtggcgatgc ccgccagcag
     1801 caggtaggcc gtctcgtggc gcgcccagtg ccgcgcctcg ccgcgccagc ccagcgccat
     1861 caggccgtac gcgatgcgct tgccgcgatc cttcgcgcgg tcgcgcagcg tcgcgaggtc
     1921 gggcagcatg ccctggtacc agaacagcgc ggagacgatc aggtacgtcg agatcgcgaa
     1981 cacgtcccac accagcgggc tgcgccattg cggccacagg ttcatgtggt cgggataggg
     2041 cgtgagccag taggcgaacc acgggcggcc cagatgcagg atcggaaaca ggccggccat
     2101 gcccgcggca aacagcgtca tcgcctcggc gaagcggttg atcgacgtgc gccagcgctg
     2161 gcgcagcagc agcaggatcg ccgagatgaa ggtgcccgca tggccgatgc cgatccacca
     2221 gacgaagttt ccgatggcaa agccccaggc gaccgggatg ttgatgcccc agatgcccac
     2281 gccctcgatg aacaggtacg tcatcgccgc caggtagacg atcgtgccgg ccatcgttgc
     2341 gaggaaggcc gccagccagg gccagcgcac cgggcgcgtc agcaccaggc cggcgatctt
     2401 gtcggtgacg ctcgcgtagc cccagcctgg gcgcagcacg ccggactcgc gggaggcggg
     2461 aacgtcggcc atcgcgtcag gcctggtcgg cggggcggat ccgcgcgagg taggtggtgc
     2521 gcggcttggt gtcgagctcc gccagcagca ggtaggccgt ctcgtggcgc gcccagtgcc
     2581 gcgcctcgcc gcgccagccc agcgcgagaa tgccgtaggc gaattgcttg aagcggctgc
     2641 cgcgcaggcc ggcgcggtcg cgcagtgtgc cga
//
LOCUS       DATCRO010000518         3496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_680910, whole genome shotgun sequence.
ACCESSION   DATCRO010000518 DATCRO010000000
VERSION     DATCRO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680910"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /gene="trpD"
                     /locus_tag="VIN75_14170"
     CDS             <1..1036
                     /gene="trpD"
                     /locus_tag="VIN75_14170"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY8975363.1"
                     /translation="PISDTEALTRVIEHREIFHDEMLALMRRIMSGEMSPTMIAAIAI
                     GLRVKKETIGEITAAATVMREFAAPVPVVDRANLVDIVGTGGDSSHTFNISTASVFVA
                     AAAGARVAKHGGRSVSSTSGSADVMEALGAHINLSPELVATCLEETGIGFMFAPNHHA
                     SMKHAAPVRKELGVRTIFNILGPLTNPAGAPNQLMGVFHPDLVGIQVRVLQRLGSEHV
                     MVVYGMNGMDEVSLSGETLVGELHKGEVREYVVHPSDFGLPVYDSRVLRVANQQESVA
                     CIRRALSNEDGPVRDVVLLNAGAALYCAGVAGSVADGVKRAREAVASGAAQAKLDQFV
                     AVTQKLKPNV"
     gene            1029..1829
                     /gene="trpC"
                     /locus_tag="VIN75_14175"
     CDS             1029..1829
                     /gene="trpC"
                     /locus_tag="VIN75_14175"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY8975364.1"
                     /translation="MSDILNRIVAVKREEIAAARPRRSEADLQELARAQSAPRGFAAA
                     MRAKVARGDAAVIAEIKKASPSKGVLREHFDPAAIAVTYAAHGAACLSVLTDVQFFQG
                     HEDYLRAARAACALPVIRKDFIVDPYQVVEARAMGADCVLLIAACLADSQMAELEAAA
                     FEQGLDVLVEVHDGAELDRALRLRTPLLGINNRNLRTFEVTLDTTLGLLDRVPADRLL
                     VTESGILSQADVARMRAADVHAFLVGEAFMRAADPGVALQALFGAPKP"
     gene            1826..2557
                     /locus_tag="VIN75_14180"
     CDS             1826..2557
                     /locus_tag="VIN75_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8975365.1"
                     /translation="MSGQADLPFGDTDAQGDVPRVRIAADLPAAFAHLPAAWREVLAG
                     WTPAAEQAVADAVRAVSGEREIAPPDPFRALRLLAPGEVKVVVFGQDPYPRPGHADGL
                     AFSAGHGKPVSLRRIFEILKADRPGWQPPERWLLDGWARQGVLMLNPVLTVEVGAAGA
                     HMNCGWQALTSDIVRVLAGRVDPPAFLLWGRPAQAFFDSACPAGSCARVLRARHPSHD
                     FKREFMADGSHFAATAGLVDWWSLA"
     gene            2658..2743
                     /locus_tag="VIN75_14185"
     tRNA            2658..2743
                     /locus_tag="VIN75_14185"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2692..2694,aa:Tyr,seq:gta)
     gene            2772..2845
                     /locus_tag="VIN75_14190"
     tRNA            2772..2845
                     /locus_tag="VIN75_14190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2804..2806,aa:Gly,seq:tcc)
     gene            2942..3016
                     /locus_tag="VIN75_14195"
     tRNA            2942..3016
                     /locus_tag="VIN75_14195"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2974..2976,aa:Thr,seq:ggt)
     gene            3065..>3496
                     /locus_tag="VIN75_14200"
     CDS             3065..>3496
                     /locus_tag="VIN75_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8975366.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLSSKFGGEA
                     KAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLSRQVGVPYIIVFLNKADMVDDA"
BASE COUNT          532 a         1209 c         1218 g          537 t
ORIGIN      
        1 gccgatttcc gacaccgaag ccctgacccg cgtcatcgag catcgcgaga tcttccacga
       61 cgagatgctc gcgctgatgc gccgcatcat gagcggcgag atgtcgccga cgatgatcgc
      121 cgccatcgcg atcggcctgc gcgtcaagaa ggagaccatc ggcgagatca ctgcggcggc
      181 caccgtgatg cgcgagttcg cggcgccggt gccggtggtc gaccgcgcaa acctggtcga
      241 catcgtgggc accgggggcg acagctcgca cacgttcaac atctccacgg cgtccgtctt
      301 cgtcgccgcc gccgccgggg cgcgcgtggc caagcatggc ggccgcagcg tgtcgtccac
      361 ctcgggcagc gccgacgtga tggaggcgct cggcgcccac atcaacctgt cgcccgagct
      421 cgtggccacc tgcctggagg agacgggcat cggcttcatg ttcgcgccca accaccacgc
      481 ctcgatgaag cacgcggcgc cggtgcgcaa ggagctgggc gtgcgcacga tcttcaacat
      541 cctgggcccg ctcaccaacc cggccggcgc gcccaaccag ctgatgggcg tgttccaccc
      601 cgacctggtc ggcatccagg tgcgcgtgct gcagcgcctg ggctccgagc acgtgatggt
      661 cgtctacggc atgaacggca tggacgaggt gtcgctgtcg ggcgagacgc tggtgggcga
      721 actgcacaag ggcgaggtgc gcgagtacgt cgtgcacccg agcgacttcg gcctgccggt
      781 gtacgactcg cgcgtgctgc gcgtggccaa ccagcaggag tcggtggcgt gcatccgccg
      841 cgcgttgtcc aacgaggacg gcccggtgcg cgacgtcgtg ctgctcaacg caggcgccgc
      901 gctgtactgc gcgggcgtgg cgggctcggt ggccgacggc gtcaagcgcg cccgcgaggc
      961 cgtggccagc ggcgcggcgc aggccaagct ggaccagttc gtggccgtca cgcagaagtt
     1021 gaagcccaat gtctgacatc ctgaaccgca tcgtcgccgt caagcgcgag gagatcgccg
     1081 ccgcccgtcc caggcgcagc gaggccgacc tgcaggagct ggcgcgcgcc cagtccgcgc
     1141 cgcgcggctt tgccgcggcc atgcgcgcga aggtggcgcg cggcgacgcc gccgtcatcg
     1201 ccgagatcaa gaaggccagc cccagcaagg gcgtgctgcg cgagcacttc gacccggcgg
     1261 ccatcgcggt cacttacgcg gcgcacgggg cggcctgcct gtcggtgctc acggacgtgc
     1321 agttcttcca gggccacgag gactacctgc gcgccgcgcg cgccgcctgc gcgctgccgg
     1381 tgatccgcaa ggatttcatc gtcgacccgt accaggtcgt cgaggcgcgc gcgatgggcg
     1441 ccgactgcgt cctgctgatc gccgcgtgcc tggccgattc gcagatggcc gagctggagg
     1501 ccgcggcctt cgagcagggg ctggacgtgc tggtcgaggt gcacgacggc gccgagctcg
     1561 atcgcgcgct gcgcctgcgc accccgctgc tgggcatcaa caaccgcaac ctgcgcacgt
     1621 tcgaggtgac gctggacacg acgctgggac tgctggaccg cgtcccggcc gatcggctgc
     1681 tggtcaccga atcgggcatc ctgtcccagg ccgacgtggc caggatgcgc gcggcggacg
     1741 tgcacgcctt cctggtcggc gaggccttca tgcgcgccgc cgatccgggc gtcgcgctgc
     1801 aggcgctgtt cggcgccccg aagccatgag cgggcaggcc gacctgccgt tcggcgacac
     1861 ggacgcgcag ggcgacgtcc cgcgcgtgcg gatcgccgcc gaccttcccg ccgcgttcgc
     1921 ccacctgccg gcggcctggc gcgaggtcct ggcgggctgg acgcccgcgg ccgagcaggc
     1981 cgtcgcggac gcggtgcggg cggtgtcggg cgagcgcgag atcgcgccgc cggatccgtt
     2041 ccgcgcgctg cggctgctgg cgcccggcga ggtcaaggtg gtcgtgttcg gccaggatcc
     2101 gtatccgcgg cccggccacg ccgacggcct ggcgttctcg gccggtcacg gcaagccggt
     2161 gtcgctgcgg cgcatcttcg agatcctcaa ggccgaccgg cctggctggc agccgcccga
     2221 gcgctggctg ctcgacggct gggcgcgcca gggcgtgctg atgctcaacc ccgtcctgac
     2281 ggtcgaagtc ggcgccgccg gcgcgcacat gaattgtggt tggcaagcgc tcacttccga
     2341 tatcgtccgc gtgcttgcgg gccgcgtcga tccgccggcc ttcctgctgt ggggcaggcc
     2401 cgcccaggca ttcttcgatt cggcctgccc ggccgggtcg tgcgcccgcg tgctgcgcgc
     2461 gcgccatccg tcgcacgact tcaagcgcga attcatggcc gacggcagcc atttcgcggc
     2521 aacggcggga ctcgtggact ggtggtcgct cgcctgacga gcagcgacag gcgtgtgacc
     2581 gcggtttcgc agccgacgac gctcgggggt ctatccaatc ccgaatctgg tgtggtatag
     2641 ttgcgggctt cgctcctgga ggggtggccg agtggttaaa ggcagcagac tgtaaatctg
     2701 cccgctaacg cgtacgctgg ttcgaatcca gcctcctcca ccaagtgtgc gaagactgtc
     2761 cctggctgca ggcgggagta gttcaatggt agaaccctag ccttccaagc taatgacgcg
     2821 ggttcgattc ccgtctcccg ctccagtgtt gcagcagccg tcaagacagt agacagtgca
     2881 gaccgttgtc gacagcggtt tgaacacggg ccctccggcc ggtgtccaag ccagtgtcga
     2941 tgcccatgtg gctcagtggt agagcactcc cttggtaagg gagaggtcgc gcgttcgatc
     3001 cgcgccatgg gcaccaccct cgttttcccc gtttcttttt cgaacgcttg actaggagcg
     3061 caagatggca aaaggcaagt ttgaacgtac caagccgcac gtgaacgtcg gcaccatcgg
     3121 tcacgtggac catggcaaga ccacgctgac ggcggccatc acgacggtgc tgtcgagcaa
     3181 gttcggcggc gaagccaagg cgtacgacca gatcgacgcg gcgcccgagg aaaaggcgcg
     3241 cggcatcacg atcaacaccg cgcacgtcga gtacgagacg gccaaccgcc actacgcgca
     3301 cgtggactgc ccgggccacg ccgactacgt caagaacatg atcaccggtg cggcccagat
     3361 ggacggcgcc atcctggtgt gctcggccgc ggacggcccg atgccccaga cgcgcgagca
     3421 catcctgctg tcgcgccagg tcggtgtgcc ctacatcatc gtcttcctga acaaggccga
     3481 catggtggac gacgcc
//
LOCUS       DATCRO010000519         9714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_692352, whole genome shotgun sequence.
ACCESSION   DATCRO010000519 DATCRO010000000
VERSION     DATCRO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692352"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VIN75_14205"
     CDS             <1..363
                     /locus_tag="VIN75_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEY8975367.1"
                     /translation="ALYGPTEVGTGQADEDVQLSTLVGKLNERFGTEFTPADQLFFDQ
                     VRETAVANEQLRQAVMANSIENFEPVFNKQLENLFIERMDGNEDIFVRLMNDEAFRSV
                     AASHLMRAVYQQVKASGK"
     gene            complement(774..850)
                     /locus_tag="VIN75_14210"
     tRNA            complement(774..850)
                     /locus_tag="VIN75_14210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Met,seq:cat)
     gene            complement(945..3746)
                     /locus_tag="VIN75_14215"
     CDS             complement(945..3746)
                     /locus_tag="VIN75_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975368.1"
                     /translation="MKPARQSLKRILTRQYVLLACVPLLLVVVLWSAVAIPATLRDIE
                     EENARTALLIRAQVELLISAPREAVARAAARVAPGTREQDVREELGEALATAPALESV
                     LLLDRDGMATIAEVRPGTQVNRKNWEGLDLSRRPFYQRARHADAPVWSETFLSPLTGR
                     ITAVVAAPAGGRMVVGDLSLDRMAQQIRASVETRDLAVIVFDSLGRVIVHPDQRRANW
                     QENLSQFELVHRALNGYSGSGEIDFDGQRWLASVAPAATTGWYVLVAQPRNALFAPVM
                     QLAGVVGIAVLVVLVVVVAIALKLAQRQAGNYRQLVSAATDLVDDRPSGPGEPGAEAD
                     LGSEEVAALWAQLRRLLDRAKEQERSARHAQAELQALLDAATEVAIIAVDANHVVRLF
                     NRGAEKMLGHRASDMLGRQCPMEMHDAAELAQRAQELSVQFKRTVRGREAITAVAERF
                     GYEVRDWTYLRKDGSRLTVSLAVTAVRAPAAGDDAPQGPRERTAAGEVVFAPGSGVVA
                     PGGDALGFLFVGTDQSERVRSGELELARRRAEAASQAKSEFLSRVSHELRTPMNAMLG
                     YAQLLQLEDAHPLDEHQRERVARIEAAGWHLVTLINDVLDLSTIESGNARIQMADIDV
                     ALVINESVRLLAPLAANAKVALAVTLPPQLPREALGVQADATRLRQVLVNVIGNAIKY
                     NLENGRVDVTVHVRAPQPAHDGDLDEETDAGRLVIEIADTGRGMTAAQLARLFSAFDR
                     LGLESSAIEGTGIGLVISRRLVERMGGEIAVDSRPDVGTRVTLTLRRAQAGPLAAAGD
                     APANLALPRSAGGQIVYVEDNPINALLMQEVVEQRPDCTLHQAVSVRDGIDMIERLRP
                     DLVLVDLHLPDGSGLQVAEWMRTRPMLARVPVVVVSADSTQTQRDAATVAGVRAYLAK
                     PIRLPEALRLIDELLPASA"
     gene            complement(3751..4860)
                     /locus_tag="VIN75_14220"
     CDS             complement(3751..4860)
                     /locus_tag="VIN75_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975369.1"
                     /translation="MPLLRLKSIPTLAALGLVALCGCQPVSTVKVGFIGELSGRVSEL
                     TIDGRNGTQLAIEALNAQSGPRYELHLHDDSPAAGTPAGIIDALADEGDAFAIGPMTS
                     VLAKAMIPEADKRQLVLISPTANSDELSGMDDWFFRVIPPAGPGAMQIADAAVGRGLL
                     TASVMAEWRNRTYSESFADTFATRFRAQGGTVSNVVRYESDGHPDYARIAGELLSAHP
                     RLVLLVCGSADASIIAQQLRRLDPDVQIAMASWAANAQLLQMGGRAIEGALVLQALDL
                     DSPEPAYVAFRKRYIERFGNPPSQAAVFSYDATMLGAEGLRRKTVGQSLRDALRAPAT
                     WPGLQQPIVLDHFGDSVSRFRLSEVRQGRFVMVGS"
     gene            complement(5017..5727)
                     /locus_tag="VIN75_14225"
     CDS             complement(5017..5727)
                     /locus_tag="VIN75_14225"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEY8975370.1"
                     /translation="MKLAQILFSQGFGTRRACEGLVGGGYVAIDGVLHDDPYEDLAPE
                     GLVLNVEGKDWPVHLKALVALNKPAGYECSKKPKHHPSVYSLLPAPLLRRDVQAVGRL
                     DEDTTGLLLFTDDGQLLHRWTSPKHHVPKVYDASCKHPVTPEQIARLLAGVVLMDDPE
                     PVRALAAEPWDGATGGLHGVRLTLAEGKYHQVKRMLAAVGNRVEALHRSRFGKLALPD
                     DLAPGAWCWLPGTQVVAD"
     gene            complement(5724..7715)
                     /gene="dsbD"
                     /locus_tag="VIN75_14230"
     CDS             complement(5724..7715)
                     /gene="dsbD"
                     /locus_tag="VIN75_14230"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY8975371.1"
                     /translation="MIRLPSMRAAWALLALLLTFAMAGPARAAGDFLDPDQAFTLSVR
                     VLDASKLELDYKVAPGYYLYRERFKFASPDAKLGEPQIPPGKKHYDTALEQNVETYHD
                     GVVVILPIVSAGKSFTLNATHQGCADKGLCYPPQPRTLAVTMKGFGADADSVKVVADA
                     DAAAAPAGVNPPAVGQVAGGMSVAPGALPDPLRSVTMAPGVAPAAQVDRAPQGGTTTI
                     LAPVPAAAPAPAPEATDSGVGAALAGGHLWLIILISIGAGLGLAATPCVWPMFPILSS
                     IIAGQGNVVTPRRGLALAAMYSLGVALVYTALGVAAGLLGKGFAGDLQKPAVLATFGV
                     LLVVLSLSMFGLYELQLPAALRDRLNAKNETLSGGQLGGVFAMGVLSALIVSPCVSAP
                     LVGVLVYIGNQHDALLGGISLFSIAVGMCVPLLVIGASAGTLLPRSGSWMDVVKRFFG
                     LMLLGVALYLVHPIVPALFSMLAWGALAIITGVVLGAFEPVTSKDYEGTARTVKGLGI
                     LFVIIGAIELVGASSGGLDPTQPLARLAGRGGASAEAGPRFDRVSSIAELDQRLQSAG
                     RPVILDFYADWCVSCKEMESQTFVDPAVRAKLDKAVLLRADVTANSADDQALLKRFHL
                     FGPPGIILFDAQGRELETVRVVGFQDAAQFSASLSAAGL"
     gene            7864..8709
                     /locus_tag="VIN75_14235"
     CDS             7864..8709
                     /locus_tag="VIN75_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8975372.1"
                     /translation="MKRHWIAACAAAMTSVLAPAAWASTSSDISVSDFVVTVKSVVPG
                     SQPGVSFAGAGGSTSVCDSSSGSPRPDQHWSAASGKAFGDASTATTPDPFAGGSATMT
                     GDVFGRGAVASASAFASSLVPASFGDGTIGLVNDVGAALFTLAPGTLLTISATVTATA
                     SASGASPDEFAESGLLMSIGDETGLGPQRAYVNFNVYASGLFGQYSDTETTFVTLTYL
                     NDTDATISGLFSGYVSSYASSGDPVSDAPEPGTSAMLLAGLLAVAGVARTRRPAPASP
                     RATRQ"
     gene            complement(8756..9148)
                     /gene="rplQ"
                     /locus_tag="VIN75_14240"
     CDS             complement(8756..9148)
                     /gene="rplQ"
                     /locus_tag="VIN75_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY8975373.1"
                     /translation="MRHRHGLRKLNRTSSHRQAMFKNMAVSLLRHEAIKTTLPKAKEL
                     RRVVEPLITLAKEPTLANRRLAFDRLRDREIVTKLFDVLGPLFKARPGGYTRILKMGF
                     RNGDNAPMAFVELVERSHSEEAVTDSEA"
     gene            complement(9276..>9714)
                     /locus_tag="VIN75_14245"
     CDS             complement(9276..>9714)
                     /locus_tag="VIN75_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEY8975374.1"
                     /translation="ENARVEQRTDLDKLVMEIETNGAISPEEAIRSSAKILVEQLAVF
                     AQLEGSDIAAFDQPAPRTAQFDPILLRPVDELELTVRSANCLKAENIYYIGDLIQRTE
                     TELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDKR"
BASE COUNT         1478 a         3394 c         3435 g         1407 t
ORIGIN      
        1 gcgctgtacg ggcccaccga ggtgggtacc ggccaagccg atgaagacgt gcagctgtca
       61 accttggtcg gcaagctcaa tgagcggttc ggcaccgagt tcacgccggc tgaccagctc
      121 ttcttcgacc aagtgcgcga aaccgcggtg gccaatgagc agctgcgcca ggcggtgatg
      181 gccaacagca tcgagaactt cgagccggtt ttcaataagc agctcgagaa cctgttcatc
      241 gagcgcatgg acggcaacga agacatcttc gtccgcctga tgaacgacga ggcgttccgt
      301 agcgtggcag ccagccacct gatgcgcgcc gtgtaccaac aggtcaaggc atcggggaag
      361 tgacatggct ctgcatcttg tgaaagcggt actggactgc ctgaaggccc gccccgagga
      421 aaaggtgacg acactggaga ttggcctacc tgctccaggt cgcgatgcta cgcctcgcac
      481 gcgaaatttc tgacaggctc ctcctgcctg gactcaagcg tgacgatgag cgcttcaacg
      541 cgcggagcgg ccacaccgaa gttcgcaaat atcgcctaac agtcatcgac acccagcggt
      601 caaaagaccg cccaacggtc gaccactcga cacagagacc aaaggtcgtc ggatgcgccc
      661 gcgactgccg tgacggcaca gacctcgaca caaacctcgt cacaacgaga tatccgggcc
      721 cggaaagcaa aacggcgctc actgggagcg ccgcatgttt cctacgtatt cggtggttgc
      781 gggggctgga tttgaaccaa cgacctttgg gttatgagcc caacgagcta ccagactgct
      841 ccaccccgcg actgaaggat gtgattatgg cataaatttg cgcccatgtc acggacgcgc
      901 ggtgaagacg tgaaaagaac acacgtgcgc acccggacgc ggcgtcacgc cgacgcgggc
      961 agcagctcgt cgatgaggcg cagcgcctcg ggaaggcgga tgggcttggc gaggtaggcg
     1021 cgcacgccgg cgacggtcgc cgcgtcgcgc tgggtctgcg tgctgtcggc cgacaccacc
     1081 acgacgggca cccgcgccag catcggtcgc gtgcgcatcc attcggccac ctgcaggccc
     1141 gaaccgtcgg gcaggtgcag gtcgaccagc acgaggtcgg gccgcaggcg ctcgatcatg
     1201 tcgatgccgt cgcgcacgct cactgcctgg tgcagcgtgc agtcggggcg ttgctcgacc
     1261 acctcctgca tcagcagcgc gttgatgggg ttgtcctcga cgtagacgat ctgcccgccg
     1321 gcgctgcgcg gcagcgccag gttcgcgggc gcgtcgcccg cggccgcgag cggcccggcc
     1381 tgcgcgcggc gcagggtcag cgtgacgcgc gtgccgacgt cggggcggct gtcgacggcg
     1441 atctcgccgc ccatgcgctc gaccaggcgg cggctgatca cgaggccgat gccggtgccc
     1501 tcgatcgcgc tggactccag ccccagccgg tcgaacgccg agaacaggcg cgcgagctgg
     1561 gcggcggtca tgccccggcc ggtatcggcg atctcgatga cgaggcgtcc ggcgtccgtc
     1621 tcctcgtcga ggtcgccatc gtgcgccggc tgcggcgcac gcacgtgcac cgtcacatcg
     1681 acgcgtccgt tctccaggtt gtacttgatc gcgttgccga tcacgttgac cagcacctgg
     1741 cgcagccgcg tggcgtcggc ctgcacgccc agcgcctcgc gcggcaactg cggcggcagc
     1801 gtcaccgcca gtgcgacctt cgcgttggcg gccagcggcg cgagcagtcg cacgctctcg
     1861 ttgatgacga gcgcgacgtc gatgtcggcc atctggatgc gggcgttgcc cgactcgatg
     1921 gtggacaggt ccagcacgtc gttgatcagc gtcacgaggt gccagcccgc ggcctcgatg
     1981 cgtgcgacgc gttcgcgctg gtgctcgtcg agcggatggg cgtcctccag ctgcagcagc
     2041 tgtgcgtagc ccagcatcgc attcatcggc gtgcgcagct cgtggctcac gcgcgagagg
     2101 aattccgact tcgcctggct cgcggcctcg gcgcgccgcc gcgcgagctc gagctcgccg
     2161 gagcgcacgc gttcggactg gtcggtgccc acgaacagga agccgagcgc gtcgccgccg
     2221 ggcgccacga cgccgctgcc cggcgcgaac accacctcgc ccgccgcagt gcgctcgcgc
     2281 gggccctgcg gcgcgtcgtc accggcggcc ggcgcgcgca cggcggtgac cgcgagcgac
     2341 acggtcaggc gcgagccgtc cttgcgcaag taggtccagt cacggacctc gtagccgaag
     2401 cgctcggcga cggcggtgat cgcctcgcga ccgcgcaccg tgcgcttgaa ctgcacgctg
     2461 agctcctgag cgcgctgcgc gagttcggcc gcgtcgtgca tctccatcgg gcattggcgg
     2521 cccagcatgt cggatgcgcg gtgtcccagc atcttctccg cgccgcggtt gaacagccgc
     2581 acgacgtggt tcgcatcgac cgcgatgatc gcgacctcgg tggccgcgtc gagcagcgcc
     2641 tgcagctcgg cctgcgcatg gcgcgcgctg cgctcctgct ccttggcgcg atcgagcagc
     2701 cggcgcagct gcgcccacag cgcggcgact tcctcgctgc cgaggtccgc ctcggccccc
     2761 ggttcgccgg ggccgctcgg ccggtcatcg acgaggtcgg tggcggcgct gaccagctgc
     2821 cggtagttgc cggcctggcg ctgcgcgagc ttcagcgcga tcgcgacgac gaccaccagc
     2881 accaccagca cggcgatgcc gacgacgccg gccagctgca tcaccggagc gaacagcgcg
     2941 ttgcgcggct gcgcgaccag cacgtaccag cccgtggtgg ccgcgggcgc gacgctcgcc
     3001 agccagcgct ggccgtcgaa gtcgatctcg cccgagcccg agtacccatt gagcgcgcgg
     3061 tgcacgagct cgaactggct caggttctcc tgccagttcg cgcgccgctg gtcgggatgc
     3121 acgatcacgc ggcccaggct gtcgaagacg atgacggcca ggtcgcgcgt ctcgacggac
     3181 gcgcggatct gctgcgccat cctgtccagc gacaggtcgc ccacgaccat gcggccgccg
     3241 gcgggagcgg cgacgacggc ggtgatgcgc ccggtcagcg gcgacaggaa ggtctccgac
     3301 cagaccggcg cgtcggcgtg gcgggcgcgt tggtagaacg ggcggcgcga caggtcgagc
     3361 ccttcccagt tcttgcggtt gacctgcgtg cccgggcgca cctcggcaat ggtggccatg
     3421 ccgtcgcgat cgagcagcag caccgattcg agcgcgggcg cggtcgccag cgcctcgccg
     3481 agctcctcgc gcacgtcctg ctcgcgcgtg ccgggcgcga cgcgcgcggc cgcgcgcgcg
     3541 acggcctcgc gcggcgcgga gatcagcagc tccacctgcg cgcggatcag cagcgcggtg
     3601 cgcgcgttct cctcctcgat gtcgcgcagc gtcgcgggga tcgcgaccgc gctccacagc
     3661 accaccacca gcagcagcgg cacgcacgcg agcagcacgt actggcgcgt gaggatgcgc
     3721 ttgagcgact ggcgcgcggg tttcatcgcc tcacgatccc accatcacga aacggccctg
     3781 gcgcacctcg ctgagccgga agcggctgac gctgtcgccg aagtgatcga gcacgatggg
     3841 ctgctgcagc ccgggccagg tggccggggc gcgcagcgcg tcgcgcaggg actggccgac
     3901 cgtcttgcgg cgcaggcctt cggcgcccag catcgtcgcg tcgtaggaga acaccgccgc
     3961 ctggctgggc gggttgccga agcgctcgat gtagcgcttg cggaacgcga cgtacgccgg
     4021 ctcggggctg tcgaggtcga gcgcctgcag caccagtgca ccctcgatcg cgcggccgcc
     4081 catctgcagg agctgcgcgt tcgcggccca cgacgccatc gcgatctgca cgtccggatc
     4141 aagccggcgc agctgctgcg cgatgatgga cgcatcggcc gaaccgcaca ccagcagcac
     4201 gagccgcgga tgcgccgaca gcagctcgcc ggcgatcctg gcgtagtccg gatggccgtc
     4261 gctctcgtag cgcaccacgt tcgacaccgt gccgccctgc gcgcgaaagc gcgtggcgaa
     4321 ggtgtcggcg aagctctcgc tgtaggtgcg gttgcgccac tcggccatca ccgatgccgt
     4381 cagcagcccg cgacccaccg ccgcgtcggc gatctgcatc gcgcccggcc cggccggcgg
     4441 gatgacgcgg aagaaccagt cgtccatgcc gctcagctcg tcggagttcg cggtggggct
     4501 gatcagcacg agctggcgct tgtcggcctc ggggatcatc gccttggcca gcacgctggt
     4561 catcggcccg atcgcgaacg cgtcgccctc gtcggcgagc gcgtcgatga tgcccgcggg
     4621 cgtgccggcc gcgggcgagt cgtcgtgcag gtgcagctcg tagcgcggcc cggactgcgc
     4681 attgagcgcc tcgatggcca gctgtgtgcc gttgcggccg tcgatggtca gttcggacac
     4741 gcggcccgac agctcgccga tgaagcccac cttgaccgtg gacaccggct ggcagccgca
     4801 cagcgcgacc aggcccagcg cggcgagcgt ggggatggac ttgagtcgca acaggggcat
     4861 ggtcaggcgc gtcaccgggg tttcagtggc gacggagggc gcctgctcca gcatagccgc
     4921 gtgcgcgagc cgcgggcaag cggcgccgcg ccttcgccgc ccccggcgtg acccagttcg
     4981 cgcgccggcc cgcgcgtgca ggccggcgca ggcgcgtcag tcggcgacga cctgcgtgcc
     5041 cgggagccag caccacgcgc cgggcgcgag gtcgtccggc agcgccagct tgccgaagcg
     5101 gctgcgatgc agcgcctcga cgcggttgcc caccgccgcc agcatgcgct tgacctggtg
     5161 gtacttgcct tcggccagcg tcaaccgcac gccgtgcagt ccgccggtcg cgccgtccca
     5221 gggctcggcg gcgagcgcgc gcaccggctc ggggtcgtcc atcagcacca cgccggcgag
     5281 cagccgcgcg atctgctcgg gcgtcaccgg gtgcttgcag gacgcgtcgt agaccttggg
     5341 cacgtggtgc ttgggcgacg tccagcgatg cagcagttgg ccgtcgtcgg tgaacagcag
     5401 caggccggtg gtgtcctcgt cgagccggcc cacggcctgc acgtcgcgcc gcagcagcgg
     5461 ggccggcagc aggctgtaga cgctcgggtg atgcttgggc ttcttggagc actcgtagcc
     5521 ggcgggcttg ttcagcgcga ccagcgcctt caggtgcacc ggccagtcct tgccctcgac
     5581 gttgagcacc aggccctcgg gggcgaggtc ctcgtacgga tcgtcgtgca gcacgccgtc
     5641 gatggccacg tagcccccgc ccacgagccc ttcgcaggcg cggcgggtgc cgaagccctg
     5701 cgagaacagg atctgcgcga gcttcacagg ccggcggccg acaggctggc cgagaactgc
     5761 gcggcgtcct ggaagccgac gacgcgcacc gtctcgagtt cgcggccctg ggcgtcgaac
     5821 aggatgatgc cgggcgggcc gaacaggtgg aagcgcttga gcagcgcctg gtcgtcggcc
     5881 gagttcgcgg tgacgtcggc acgcagcagc acggccttgt ccagcttggc gcgcacggcc
     5941 gggtcgacga aggtctgcga ctccatctcc ttgcacgaga cgcaccagtc ggcatagaag
     6001 tccaggatca ccggccggcc ggcggactgc agccgctggt cgagttcggc gatgctggag
     6061 acgcggtcga agcgcgggcc ggcctcggcg ctcgcgccgc cgcgccctgc gaggcgggcc
     6121 agcggttgcg tcggatcgag gccgccgctg gacgcgccga cgagttcgat cgcaccgatg
     6181 atgacgaaca ggatgccaag gcccttgacg gtgcgcgccg tgccctcgta gtccttgctg
     6241 gtgaccggct cgaaggcgcc cagcacgacg ccggtgatga tcgccagcgc gccccaggcc
     6301 agcatcgaga acagcgccgg gacgatcgga tgcaccaggt acagcgcgac gcccagcagc
     6361 atcaggccga agaagcgctt gaccacgtcc atccagctgc cgctgcgggg cagcagggtg
     6421 ccggccgagg caccgatgac cagcagcggc acgcacatgc ccacggcgat cgagaacagc
     6481 gagatgccgc ccagcagcgc gtcatgctgg ttgccgatgt acaccagcac gccgaccagc
     6541 ggcgccgaca cgcacgggct cacgatcagc gccgaaagca cgcccatcgc gaacacgccc
     6601 ccgagttggc cgcccgacag cgtctcgttc ttcgcattga ggcggtcgcg cagcgccgcc
     6661 ggaagctgca attcgtacaa gccgaacatg gacagcgaca gcacgaccag caacacgccg
     6721 aaggtggcca gcacggccgg cttctgcagg tcgcccgcga agcccttgcc gagcaggccg
     6781 gcggcaacac ccagcgcggt gtagaccagc gcgacgccga gcgaatacat ggccgccagc
     6841 gcgagcccgc gccgcggcgt cacgacattg ccctggcccg cgatgatgga cgacaggatc
     6901 gggaacatcg gccagacgca gggcgtggcc gccaggccca ggccggcgcc gatcgagatg
     6961 aggatgatga gccacaggtg gccgcccgcg agcgcggccc cgacgccgct gtcggtggct
     7021 tcgggcgccg gtgccggcgc ggccgccggc accggcgcca ggatcgtcgt cgtcccgccc
     7081 tgcggcgcgc gatcgacctg cgccgccggt gccacgcccg gcgccatggt cacgctgcgc
     7141 agcgggtcgg gcagcgcgcc cggcgcgacg ctcatgccgc cggccacctg gcccacggcg
     7201 ggcgggttca cgccggccgg cgcggccgcc gcgtcggcgt cggccaccac cttgaccgaa
     7261 tcggcatcgg cgccgaagcc tttcatcgtc acggccagcg tgcgcggctg cggcgggtag
     7321 cacaggccct tgtcggcgca gccctggtgc gtggcgttca gcgtgaacga cttgcccgcg
     7381 gagacgatcg gaaggatcac gaccacgccg tcgtggtacg tctcgacgtt ctgctccagc
     7441 gcggtgtcgt agtgcttctt gccgggcggg atctgcggct cgcccagctt ggcgtcgggc
     7501 gacgcgaact tgaagcgctc gcgatacagg tagtagccgg gcgcgacctt gtagtccagc
     7561 tcgagcttgc tggcatcgag cacgcgcacc gacagcgtga acgcctggtc ggggtcgagg
     7621 aaatcgcccg cggcgcgcgc cgggccggcc atggcgaacg tcagcaggag agcgaggagc
     7681 gcccaggcgg cccgcatcga aggcagtcga atcatggagt tgtagagtcg gccggagccg
     7741 gctcgttccc gtggaagaat ggattgtcgc cggtccccgg ccgaccttcg gggggccccc
     7801 ggattgcccc gattcgaggc ggacgactcg ggggccgccc gacaacgaca gtctggagga
     7861 gacatgaagc ggcattggat cgcggcctgc gcggccgcga tgacgagcgt cctcgccccc
     7921 gcagcgtggg cgagcacctc gtcggacatc agcgtgagcg atttcgtcgt cacggtgaag
     7981 tccgtcgttc ccggcagcca gcccggcgtg agcttcgccg gcgcgggagg cagcacctcg
     8041 gtctgcgaca gctcgtccgg cagcccgcgt cccgaccagc actggtcggc cgcgagcggc
     8101 aaggccttcg gtgacgcgtc cacggcaacg acgcccgatc cgttcgccgg cggctccgcc
     8161 accatgaccg gcgacgtctt cggccgcggc gccgtggcca gcgccagcgc gttcgcgagc
     8221 agcctggtgc cggcatcgtt cggcgacggc acgatcgggc tggtcaacga cgtcggcgcc
     8281 gcgctgttca cgctcgcgcc cggcacgctc ctgacgatct cggccacggt gaccgccacg
     8341 gcctcggcca gcggcgccag ccccgacgag ttcgccgaga gcgggctgct gatgtccatc
     8401 ggcgacgaga ccggcctggg cccgcagcgc gcatacgtca acttcaacgt ctacgcctcg
     8461 gggctgttcg gccagtacag cgataccgag acgacgttcg tcacgctcac ctacctcaac
     8521 gacaccgacg cgacgatctc gggcctgttc agcggctacg tctcgtcgta cgcgagttcc
     8581 ggcgatcccg tcagcgatgc gcccgagccc ggcacgtccg cgatgctgct ggcgggcctg
     8641 ctggccgtgg cgggcgtcgc gcgcacgcgc cggcccgcgc ccgcttcccc gcgcgcgacg
     8701 cgccaatgaa aaaggccggc atgtgccggc cttcgtgtca ggaagcggga gccgctcagg
     8761 cttccgaatc ggtgacggct tcttccgagt gcgagcgctc gaccagctcg acgaacgcca
     8821 tcggcgcgtt gtcgccgttg cggaagccca tcttcaggat gcgggtgtag ccgcccggac
     8881 gcgccttgaa cagcgggccc agcacgtcga acagcttggt gacgatctcg cggtcgcgca
     8941 ggcggtcgaa ggccaggcgg cggttcgcca gcgtcggctc cttggccagc gtgatcaggg
     9001 gctcgacgac gcggcgcagt tccttggcct tgggcagcgt ggtcttgatg gcttcgtggc
     9061 gcagcagcga gacggccatg ttcttgaaca tggcttggcg gtgcgacgag gtgcggttga
     9121 gcttgcgaag gccgtgacga tggcgcatgg tgatttcctt tggtctcttt attgaacccc
     9181 ggccggatca ggtaccgggg gtttgcacac ggcgcccgtc tggatcgacg ggcccggcag
     9241 cggggaacct acaaaaaagc gatcaggaca gcggcttagc gcttgtccag accttgcggg
     9301 ggccagttct cgagacgcgc gcccagcgtg aggccgcgcg aagcgaggac ttccttgatt
     9361 tcgttgagcg acttgcgacc caggttgggg gtcttgagca gctcggtctc ggtacgctgg
     9421 atcaggtcgc cgatgtagta gatgttttcg gccttcaggc agtttgccga acgcaccgtg
     9481 agctcgagct cgtcgaccgg acgcagcagg atcgggtcga actgggccgt gcgcggggcc
     9541 ggctggtcga acgcggcgat gtccgaacct tccagctgcg cgaacacggc gagctgttcc
     9601 accaggatct tggccgacga acggatcgct tcctcgggcg agatggcgcc gttggtctcg
     9661 atctccatca ccagcttgtc gaggtcggtg cgctgctcga cgcgggcgtt ctcg
//
LOCUS       DATCRO010000520         5935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_928221, whole genome shotgun sequence.
ACCESSION   DATCRO010000520 DATCRO010000000
VERSION     DATCRO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928221"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VIN75_14250"
     CDS             <1..448
                     /locus_tag="VIN75_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8975375.1"
                     /translation="SPGTLATPVPASPDGAARWGTVMHQDDIRAAVQRHWAASDAGDF
                     ATEHEIYDQDAVLDYPQSGERIRGSQRIQASRMAQPDRKRFSLRGLKGEGGLWVSELA
                     MGYGDRPFHMVSIMEFAGGKVIHETQYFAEPFAPGASRARWVERIE"
     gene            complement(463..1272)
                     /locus_tag="VIN75_14255"
     CDS             complement(463..1272)
                     /locus_tag="VIN75_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8975376.1"
                     /translation="MGFRLVTLNLNGIRSAHNKGFLAWAESMKADCMGVQEVKAQHEH
                     VHETFDTLDGMAGRFHYAVKKGYSGVGLYSRVAPTEVVTGFGDAEFDDEGRYIEFRFD
                     KPSEGLSHPFEKLSIISCYFPSGSSGEERQAAKFRFLDLIYPHLMALKGQREFILVGD
                     INIAHKNIDLKNWKGNQKNSGFLPEEREWMTKLLTDGGLVDVFRTLNDKEEQYTWWSN
                     RGQAWAKNVGWRLDYHLATPGVAALARKEAIHIESRFSDHAPLTIDYDFTL"
     gene            1284..1961
                     /gene="pyrE"
                     /locus_tag="VIN75_14260"
     CDS             1284..1961
                     /gene="pyrE"
                     /locus_tag="VIN75_14260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY8975377.1"
                     /translation="MTAPTNDLLAQEFVQFSVEAGVLRFGDFKTKAGRQSPYFFNTGF
                     FDDGMKLMRLGEFYARRLLASGVEFDMLFGPAYKGITLAAAVAIGLARHGKNVTYAYN
                     RKEAKDHGEGGTLVGAPVRGRVMIIDDVITDGASKREAADLIRAVGAEPVGVAITMDR
                     QERARDEGEDRRSGVQYVEQTLGLKVVAIATLADLMQLLATTGDPSLAASKDAVAAYR
                     ARYGIDA"
     gene            1975..2331
                     /locus_tag="VIN75_14265"
     CDS             1975..2331
                     /locus_tag="VIN75_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975378.1"
                     /translation="MTHRPVCPSRAIRMAMGAALLLAAAGAQAMTVSYQCTGRRLLTA
                     ELNPRKGQLHFEGHDWTVLRVPDKNMARYVSKQDDIEVITKDRAMTFTHGGETLQCLL
                     YSDALPGDAPRTRPTN"
     gene            2333..2809
                     /locus_tag="VIN75_14270"
     CDS             2333..2809
                     /locus_tag="VIN75_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8975379.1"
                     /translation="MSTSDPSSGETILREARMSWWALWPRVVLGLVGVVLVAWGLLRQ
                     DHLGYGVAGFGALAFLWFVGGALLRRSAMNVKLTDRYVVLQRGLVSRHTSTVMLNRVE
                     SLDVDQSLWQRMTGYGTLTIRGTGSEDLRLAGLTDPVGFQAAARQAINAASGRASF"
     gene            complement(2970..3851)
                     /locus_tag="VIN75_14275"
     CDS             complement(2970..3851)
                     /locus_tag="VIN75_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975380.1"
                     /translation="MRVFITGATGFIGGVVVPELLRAGHQVLGLARSDAGADVLRAQG
                     AGVHRGTLEDLASLRRGAEQADGVIHAGFIHDFSKFAENCEIDRRAIEALGDVLAGSG
                     RPLVVTAGIPGVAGRASTEDDPPQQMTPRVSEQTAVAQAARDVRATTIRLPQVHDRDK
                     AGLVSYLIMIARQKGVSAYVGDGANRWSAVHRQATASLYRLALESGVAGSRYHAVEEE
                     GVPLKAIAQAIGRGLGVPVKSLSPAQAAAHFGPMGHFAGMDNPASSALTRERLGWTPG
                     GHPGLIDDLDHSTAYAA"
     gene            4129..5061
                     /locus_tag="VIN75_14280"
     CDS             4129..5061
                     /locus_tag="VIN75_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8975381.1"
                     /translation="MDPLSDVLSLLKPRSFMSGGVDYGPDWSLRFGPHDGIKYQAMLS
                     GGCWLQVEGLEPVRVEAGDCYLLPRGLPFRLASDLALPSIDAVELLKLPLNGGMRTIN
                     GGGQCVALGGHFAFEGRHAPMLLGMLPPIVHLRSEADKAAMRWSIERLMREIREPQPG
                     GFLIAQQLAHMLLVQALRLHLNDGAADGVGWLFALADRQMSAAMNAMHAEPGRRWTIA
                     ALAKEAAMSRSTFALKFKRTVGASPMDYLTRWRMLVAADRLAGGGETIATLAPALGYE
                     SESAFSTAFKRVMGCAPRHYGRGDATPDVALVPA"
     gene            complement(5217..>5935)
                     /gene="gatB"
                     /locus_tag="VIN75_14285"
     CDS             complement(5217..>5935)
                     /gene="gatB"
                     /locus_tag="VIN75_14285"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229956.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY8975382.1"
                     /translation="IVQATVLFDPDSGETRMMRTKEDAQDYRYFPDPDLPPLVISREW
                     VEQVRASMPELPRAMAERFVKADGLPEYDAAMMTQSLATARFYEAARDAGAPAKLVAN
                     WTMGEISRRLNSENLSIEESPVDAPLLASLIKRIQDGTISNNAARQVFEALWTREKGS
                     AGNVDDVIDRLDVRQNNDTGALEAILDEVLKANQKSVDEFKAGKEKAFQALVGQAMKA
                     SKGKANPAQVNELLKKKLAG"
BASE COUNT          858 a         2022 c         2076 g          979 t
ORIGIN      
        1 gtccccgggc acgctcgcga cgccggtgcc cgccagtccg gacggagcgg cgcgatgggg
       61 gaccgtcatg catcaggacg acatccgggc tgcagtgcag cgccactggg ccgcctcgga
      121 cgccggtgat ttcgcgaccg agcacgagat ctatgaccag gacgccgtgc tcgactatcc
      181 gcagtcgggc gaacgcatcc gcggcagtca gcggatccag gcgtcgcgca tggcgcagcc
      241 ggaccgcaag cgcttctcct tgcgcggcct gaagggcgag ggcggcctgt gggtcagcga
      301 actggccatg ggctacggcg accggccgtt ccacatggtc agcatcatgg aattcgccgg
      361 cggcaaggtg atccacgaga cgcagtactt tgccgagccg ttcgccccgg gcgcttcgcg
      421 tgcgcggtgg gtcgaacgca tcgagtgagc ccgccgacgc gtctacagcg tgaagtcgta
      481 gtcgatcgtc agcggcgcgt ggtcgctgaa gcgcgactcg atgtggatgg cttccttgcg
      541 cgccagcgcg gcgacgccgg gcgtggccag gtggtagtcc aggcgccagc ccacgttctt
      601 cgcccaggcc tggccgcggt tgctccacca cgtgtactgc tcttccttgt cgttgagcgt
      661 gcggaacacg tcgaccaggc cgccgtcggt cagcagcttg gtcatccact cgcgctcctc
      721 gggcaggaag ccggagttct tctggttgcc cttccagttc ttcaggtcga tgttcttgtg
      781 cgcgatgttg atgtcgccga ccaggatgaa ctcgcgctgg cccttgagcg ccatcaggtg
      841 cgggtagatc aggtcgagga agcggaactt ggcggcctgg cgttcctcgc cggacgagcc
      901 gctggggaag tagcagctga tgatgctgag cttctcgaac ggatggctca ggccttcgga
      961 cggcttgtcg aagcggaact cgatgtagcg gccctcgtcg tcgaactcgg cgtcgccgaa
     1021 gccggtgacg acctcggtgg gcgcgacgcg gctgtacagg cccacgccgg agtagccctt
     1081 cttgaccgcg tagtggaagc ggccggccat gccgtccagg gtgtcgaagg tctcgtgcac
     1141 gtgctcgtgc tgcgccttga cctcctgtac gcccatacaa tcggccttca tggactccgc
     1201 ccatgcaaga aagcccttgt tgtgggccga acggatcccg ttcaggttca atgtcaccaa
     1261 gcgaaatccc aaggcactac tccatgacag cacccaccaa cgacctgctc gcgcaggaat
     1321 tcgtccagtt ctcggtggag gccggtgttc tgcggttcgg tgatttcaag acgaaggccg
     1381 gacgccagtc gccctacttc ttcaacaccg ggttcttcga cgacggcatg aagctcatgc
     1441 gtctgggcga attctatgca cgccggctgc tcgcttccgg cgtggagttc gacatgctgt
     1501 tcggcccggc ctacaagggc atcacgctgg ccgccgcggt ggccatcggg ctcgccaggc
     1561 acggcaagaa cgtcacctac gcgtacaacc gcaaggaagc caaggatcac ggcgagggcg
     1621 gcacgctggt gggcgcgccg gtcaggggcc gcgtgatgat catcgacgac gtcatcaccg
     1681 acggcgcctc caagcgcgag gccgccgacc tgatccgcgc cgtcggcgca gagccggtgg
     1741 gcgtggccat cacgatggat cgccaggaac gcgcacgcga cgagggcgag gaccgccgct
     1801 cgggcgtgca gtacgtcgag cagacgctgg gcctgaaggt ggtggccatc gccacgctgg
     1861 ccgacctgat gcagctgctc gccaccaccg gcgacccgtc gctggccgcc agcaaggatg
     1921 cggtcgccgc ctatcgcgcc cgctacggca tcgacgcctg aaaggtcccg tcccatgacc
     1981 catcgccccg tttgcccgtc acgcgccatc cggatggcga tgggggcggc gctgctgctg
     2041 gccgccgccg gcgcgcaggc catgaccgtc agctaccagt gcaccggccg ccggctgctc
     2101 acggcggaac tgaacccgcg caagggccag ctgcacttcg agggccacga ctggaccgtg
     2161 ctgcgcgtgc ccgacaagaa catggcgcgc tacgtcagca agcaggacga catcgaggtc
     2221 atcacgaagg accgcgcgat gaccttcacg cacggcggcg agacgctgca gtgcctgctg
     2281 tactccgacg cgctgccggg cgatgcgccg cgcaccaggc ccacgaactg acatgagcac
     2341 cagcgacccg tcctccggcg agaccatcct gcgcgaggcc cgcatgtctt ggtgggcgct
     2401 gtggccgcgc gtcgtgctcg ggctcgtggg cgttgtgctc gtcgcctggg gcctgctgcg
     2461 ccaggatcac ctgggctacg gcgtggccgg cttcggcgcg ctggccttcc tgtggttcgt
     2521 gggcggtgcg ctgctgcgcc gcagcgccat gaacgtcaag ctgaccgacc gctacgtcgt
     2581 gctgcagcgc gggctcgtga gccgccacac gtccaccgtg atgctcaatc gcgtcgagag
     2641 cctcgacgtc gaccagagcc tgtggcaacg catgaccggc tacggcacgc tgacgatccg
     2701 tggcaccggc agcgaggacc tgcgcctggc ggggctgacc gatccggtcg ggttccaggc
     2761 ggcggcgcgg caggcgatca acgcggcgag cggcagggcc tcgttctagt cgtcgcgccg
     2821 cgaaaggctg tcattccgcg cggagccaag cggagtcgca gaatccatcc tggaggcggc
     2881 gctgcccgcc gcgtggatcc tgcgactgcg cgcagcatga catgggatga gcgcggcgcg
     2941 atcacctggc gtggccatcg agggggcgcc taggccgcat acgccgtcga gtggtccaga
     3001 tcgtcgatga gccccggatg ccctcccggc gtccagccca ggcgctcgcg ggtcagcgcg
     3061 ctcgacgccg ggttgtccat gcctgcgaag tgccccatcg gcccgaagtg cgcggcggcc
     3121 tgcgccggcg acagcgactt caccggcacg cccagcccgc ggccgatggc ctgcgcgatc
     3181 gccttcaacg gcacgccttc ctcctcgacc gcgtgatagc ggctgcccgc cacgccgctt
     3241 tcgagcgcca gccggtacag cgatgccgtg gcctgccgat gcaccgccga ccagcggttg
     3301 gcgccgtcgc cgacgtaggc cgacacgccc ttctgccggg cgatcatgat caggtagctg
     3361 accagcccgg ccttgtcgcg gtcgtgcacc tgcggcaggc ggatcgtcgt cgcacgtacg
     3421 tcgcgggcgg cctgcgcgac ggccgtctgc tcggacaccc gcggcgtcat ctgctgcggc
     3481 gggtcgtcct cggtgctcgc gcgcccggcc acgcccggga tgcccgcggt cacgaccagc
     3541 ggccggccgc tgcccgcgag cacgtcgccg agggcctcga tggcgcggcg gtcgatctcg
     3601 cagttctcgg cgaacttcga gaagtcgtgg atgaacccgg cgtggatgac gccgtccgcc
     3661 tgctcggcgc cgcggcgcag gctggccagg tcctccagcg tgccgcggtg gacgccagcg
     3721 ccttgcgcgc gcagcacgtc cgcgcccgcg tccgagcgcg cgaggcccag cacctggtgg
     3781 ccggcgcgca gcagttcggg cacgaccacg ccgccgatga acccggtcgc gccggtgatg
     3841 aagactcgca tggggttctc tccttgtggg gtgtcaggta tcgcggtgtc aggtcttgcc
     3901 ttttgcgcga acgcatcgat ggggtcaggt cttgaacgtt gcggggtcag gtcttgaaag
     3961 ttgcggggtc aggtcttgat tcgtgcagtg cacgattcaa gacctgaccc cgccttcggt
     4021 gcacgccgca agacctgaca ccctgtcgat gagggcactc tagtgactcg aatcgataca
     4081 atccatgctt ttcagtccgc aaaatcttcg tgatcgtccg aaatggccat ggatccgttg
     4141 tctgatgtcc tgtcgctgct caagccgcgc agcttcatgt cgggcggggt ggactacggc
     4201 ccggactggt cgctgcgctt cgggccgcac gacggcatca agtaccaggc gatgttgtcg
     4261 ggcggctgct ggctgcaggt cgaggggctg gagcccgtgc gcgtggaggc cggcgactgc
     4321 tacctgctgc cacgcgggct gccgttccgc ctggccagcg acctggcgct gccgtcgatc
     4381 gacgccgtgg agctcctgaa gctgcccctg aacggcggca tgcgcacgat caacggcggc
     4441 ggccagtgcg tggcactggg cggacatttc gcgttcgagg ggcggcacgc gccgatgctg
     4501 ctcgggatgc tgccgcccat cgtccacctg cggtcggagg cggacaaggc ggcgatgcgc
     4561 tggtcgatcg aacgcctgat gcgcgagatc cgggagccgc agcccggcgg cttcctgatc
     4621 gcgcaacagc ttgcgcacat gctgctggtg caggcgctgc ggctgcacct gaacgacggc
     4681 gcggccgacg gcgtgggctg gctgttcgcg ctggccgacc gccagatgag cgccgcgatg
     4741 aacgcgatgc acgccgagcc gggccggcgc tggacgatcg cggcgctcgc caaggaggcg
     4801 gcgatgtcgc gctccacctt cgcgctgaag ttcaagcgca cggtgggcgc ctcgccgatg
     4861 gactacctca cgcgctggcg catgctcgtg gccgccgatc gcctcgccgg cggcggcgag
     4921 acgatcgcga cgctggcccc cgcgctcggc tacgaatcgg agagcgcgtt cagcacggcg
     4981 ttcaagcgcg tgatgggctg cgcgccgcgg cactacgggc ggggcgacgc gacgcccgac
     5041 gtcgcgctgg tgccggcgta gggcggtagg gcgggttagc cgcaggcgta acccgccgtt
     5101 cggtggcgcg ctgccctgcg taccttgcat tccggagcgc cgggcaggcc ggcacgtcgg
     5161 caagcggcgg gttacggctg cgcctgaccc gccctacggc cgcacggcga gcccgatcag
     5221 cccgccagct tcttcttgag cagctcgttg acctgcgccg ggttggcctt gcccttgctg
     5281 gctttcatcg cctggcccac gagcgcctgg aaggcctttt ccttgccggc cttgaactcg
     5341 tcgaccgact tctggttggc cttgaggact tcgtcgagga tggcttcgag cgcaccggtg
     5401 tcgttgttct ggcgaacgtc cagccggtcg atcacgtcgt cgacattgcc cgccgagccc
     5461 ttctcgcgcg tccacagcgc ctcgaacacc tgcctggcgg cgttgttgga gatggtgccg
     5521 tcctggatgc gcttgatcag cgatgcgagc agcggcgcgt cgacgggact ctcctcgatc
     5581 gagaggttct cgctgttcaa gcggcgcgag atctcgccca tcgtccagtt cgcgacgagc
     5641 ttggccggcg cgccggcatc gcgcgcggcc tcgtagaagc gcgcggtggc cagcgactgc
     5701 gtcatcatcg cggcgtcgta ctcgggcagg ccgtcggcct tgacgaagcg ttcggccatc
     5761 gcgcgcggca gctcgggcat gctcgcgcgc acctgctcga cccactcgcg cgagatcacc
     5821 agcggcggca ggtcggggtc ggggaagtag cgatagtcct gcgcgtcttc cttggtgcgc
     5881 atcatgcgcg tctcgccgga gtcggggtcg aacagcacgg tggcctgcac gatgg
//
LOCUS       DATCRO010000521         7378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_703419, whole genome shotgun sequence.
ACCESSION   DATCRO010000521 DATCRO010000000
VERSION     DATCRO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703419"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2260
                     /locus_tag="VIN75_14290"
     CDS             98..2260
                     /locus_tag="VIN75_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8975383.1"
                     /translation="MTTWRSRLAAWPGIAFFRLRLFFRGVFLLLALATIAIALSVLQD
                     EKELSHRSYRQGFAKTMGQVAAQLRHPAGQLALLNPPGPSASAATAFPGAVHPLVLPF
                     SALDFDDHNKVEQAVEMAGCTRQYPDDAALCVALGSNPWAGGFVYVVGHAPGPPPVAH
                     RTGDHDLSQSTRLRVDVALRGRRYAWVAPFEPEPATAVNVVRGRLTGFALDDDGSVLP
                     RPVRDFRGWLWQGGPCTDGGKDIATCARRAFFSVRLPVEVLHEDVLVSAHPVWPPADM
                     NRIDVRVQWLAPGDGAPLFDSARPGATAPFSLSDLSAQLLPGETLQLRRIDGRTSAPL
                     ATIKGAPPPAEPSSSWMPQAVNDFLGYAVRGLLRRLPIEDEDVPIEDTETIRTAAGSV
                     ELHGYGDERSANRALAQVATRVSWFVIAMLVALALAWGAIEWTIIRRITMLTKRSRAV
                     TAAVAEDASASSRWSALDIADLRSPDELGVLASNLAELLQRVDESMQRERLRAEREKD
                     QWMAVGHEIMSPLQSLIALHGAPDDASRRYILRMQQAIRVLYGSASPSEAFETTTLQL
                     ATLELDAFCAAVAANAPHAGLFDVVYDGPGEPVVVRADEYSLEDVVTHVLQNAQRHRR
                     AGTRITLRLRADEARATLELHNEGEPIPPELIDRIFEYGVSGTQDDEGAGVDADRHAP
                     QRGQGLFVAKTYMAKMGGTIAAENVPGGVRFVLTLPRA"
     gene            2363..3307
                     /locus_tag="VIN75_14295"
     CDS             2363..3307
                     /locus_tag="VIN75_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975384.1"
                     /translation="MRRHLCPRAFAAALAFATLLPAFAQSDAPATTPRRFERLRQAMA
                     GDLEDGSAQDRSARGAPSGTRLVPDIPYGPDPAQRLDVYVSAQPQAGTAPLPVIFFVH
                     GGAWARGDKTNTRVVEPKIAHWVDQGFVVISANYRMLPTPVAQQADDVAAAIAFAQSQ
                     AGLWGGDGRRFILMGHSAGAHLVALIAAGARTATRPQPWRGAVLLDSAAFDVTTLMTH
                     RHFGLYDHAFGSDRAYWAAMSPIEQLAHATAPILAVCSSRRQESCGQADRFAGKANGL
                     GGKVRVLREDLSHKEINATLGAASDYTSQVDAFLASVR"
     gene            complement(3314..3832)
                     /locus_tag="VIN75_14300"
     CDS             complement(3314..3832)
                     /locus_tag="VIN75_14300"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8975385.1"
                     /translation="MNQMTPHLSNAASAATATSAGARIAVICASWHTDIVDRAKQSLL
                     GEFDRSPTPPGKVDQYEVPGAFEIPLLAQTLARSGRYDAIVACALVVNGGIYRHEFVG
                     SAVIDGLMRVQLDTGVPVMSAVLTPRDFHEHDEHRRFFAEHFVKKGVEVAQACLMTIA
                     GLRRANLALAAA"
     regulatory      complement(3928..4083)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4160..5665)
                     /locus_tag="VIN75_14305"
     CDS             complement(4160..5665)
                     /locus_tag="VIN75_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY8975386.1"
                     /translation="MTEPKTAADAKPDADKARQTQRDAVEKLLATAPMRSTGSIKLGG
                     RKLEYKVEGAFVPVVNATQTDKRGEPEAAVFTTAYQLDRKAGDTRPRPVCFAFNGGPG
                     SASIWLQLGAMGPKRLRIGNDGSMLPPPYEVEDNPLSWFEHFDLVFVDPPHTGYSITA
                     SDDARKKMLSVDGDVACLTDVIRTWLSRHQRWTSPIYVAGESYGTTRGAAIADKLADQ
                     GVSLAGLILISCAMDLQSIVFEARNDLPYALFLPAFAGVAQYHGCLKGPPSKSAAAAR
                     ALATDFVESDYLRVLHKGARATDDERAKVARRMAELTGLSQTLVEHRNLRISDNTFFT
                     ELLRDRGQIVGRLEARVAGPMGLSRTHEWDFDPGMESLVGPYTMGGMGYFIGTLGFDP
                     SWPYEVLSMDTNKHWNWSRGDVQGNSFCCTSPDLSRALRRNPHLRVLVASGHFDLGTP
                     STASDWSLAQLDIPADVRRRVTHQYYDAGHMMYTRDEDLAKLKKDLAAWLP"
     gene            complement(5754..6512)
                     /locus_tag="VIN75_14310"
     CDS             complement(5754..6512)
                     /locus_tag="VIN75_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEY8975387.1"
                     /translation="MSDPNSLIPPPLTPAGDVDAALAGAGFAVVGPEGLAALAGATPA
                     DFDAWRPFWNRLPADAYLLDGGKYRQRRHGVFVVDGDTVSAAPHRPHWQPVDYNALHG
                     GIERHFEPIEAGLAGDPAWRRLLAGLAARASALKGAQRWFVEAHTFRIDTASGIGRPT
                     PEGAHRDGVDLVSVTLVDRHGIKGGESRVFQAASSAGLRFTLSQPWTTVLLDDARVIH
                     ETTPIQPLRSGEPGWRDTLVLTFRAGAFQGPAAA"
     gene            6671..>7378
                     /locus_tag="VIN75_14315"
     CDS             6671..>7378
                     /locus_tag="VIN75_14315"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8975388.1"
                     /translation="MSASKGHSRPSVVPAAPGPDDGAAPAALPAALLAWGRTAPVALA
                     AIAADGTVAWVNPAFERLAGLLSEQATGQPLETLLADANWPRWLPPPGTLARRRLLRR
                     DATALHCDAQVDAIEGGLRLVTLVGRDDEARIDLEACRLKALLDFVQTHARIGLWERN
                     VRTREGKWDPHMYRFFGIDPRHGPPSIAQIAQATVPQDRLFQVLDDSMRKAGSYSHRY
                     RLFTADGGLRRVQAHWEV"
BASE COUNT          983 a         2677 c         2688 g         1030 t
ORIGIN      
        1 gcactgcagc cggcccccca ccggggcgcg gggacctgcc ccggagcggc cgagcgcggc
       61 gtccgcgtgc tcgtgcccct cgagtgaggg gcccgcgttg acgacgtggc gctcccgcct
      121 ggccgcctgg cccggcatcg cgttcttccg cctgcgcctg ttcttccgcg gcgtgttcct
      181 gctgctggcg ctggcgacga tcgccatcgc gctgtcggtg ctgcaggacg agaaggagct
      241 gagccaccgc agctaccgcc agggcttcgc caagacgatg ggccaggtgg ccgcgcagct
      301 gcgccacccc gcgggccagc tcgcgctgct aaacccgccg gggccttcgg cctcggccgc
      361 caccgccttc ccgggcgcgg tgcatccgct ggtgctgccg ttctcggcgc tggacttcga
      421 cgaccacaac aaggtcgaac aggccgtcga gatggccggc tgcacgcgcc agtatcccga
      481 cgacgcggcg ctgtgcgtgg ccctgggcag caacccgtgg gccggcggct tcgtgtacgt
      541 ggtggggcat gcgcccgggc cgccgcccgt cgcgcaccgg accggcgacc acgacctctc
      601 gcagtcgacg cgcctgcgcg tagacgtcgc gctgcgcggc cgccgctacg cctgggtcgc
      661 gccgttcgag cccgagcccg ccacggcggt caatgtcgtg cgtggccgcc tgacgggctt
      721 cgccctcgac gacgacggca gcgtgctgcc gcggccggtg cgcgacttcc gcggctggct
      781 gtggcagggc ggcccgtgca ccgacggcgg caaggacatc gccacctgcg cgcggcgcgc
      841 gttcttctcg gtgcggctgc cggtggaggt gctgcacgag gacgtgctcg tgtcggcgca
      901 tccggtgtgg ccgccggccg acatgaatcg catcgacgtc cgcgtgcaat ggctcgcgcc
      961 gggcgacggc gcgccgctgt tcgacagcgc gcgccccggc gcgacggcgc cgttctcgct
     1021 gtccgacctc agcgcgcagc tgctgcccgg cgagaccctg caactgcgca ggatcgatgg
     1081 caggaccagc gcgccgctgg ccacgatcaa gggcgcgccg ccgccggccg agccgtcctc
     1141 cagctggatg ccgcaggccg tcaacgactt cctgggctac gccgtgcgtg gcctgctgcg
     1201 ccggctgccg atcgaggacg aggacgtgcc catcgaggac accgagacga tccgcaccgc
     1261 cgccggcagc gtcgagctgc acggctacgg cgacgagcgc agcgccaacc gcgcgctggc
     1321 gcaggtggcc acgcgggtgt cgtggttcgt catcgcgatg ctggtggcgc tcgcgctggc
     1381 ctggggcgcc atcgagtgga cgatcatccg tcgcatcacc atgctgacca agcgctcgcg
     1441 cgcggtcacg gccgcggtgg ccgaggacgc gagcgccagc agccggtggt cggcgctgga
     1501 catcgccgac ctgcgcagcc ccgacgagct gggcgtgctc gcgagcaacc tcgccgagct
     1561 gctgcagcgc gtcgacgaga gcatgcagcg cgaacgcctg cgcgccgagc gcgagaagga
     1621 ccagtggatg gccgtgggcc acgagatcat gtcgccgctg cagtcgctca tcgctctgca
     1681 cggcgcgccc gacgacgcaa gccggcgcta catcctgcgc atgcagcagg cgatccgcgt
     1741 gctgtacggc agcgcgtcac ccagcgaggc tttcgagacg accacgctgc agctagccac
     1801 gctggagctc gacgcgttct gcgccgcggt ggccgccaac gcgccgcacg cgggcctgtt
     1861 cgacgtcgtc tacgacggcc ccggcgagcc ggtggtcgtg cgcgccgacg agtactcgct
     1921 ggaagacgtc gtcacgcacg tgctgcagaa cgcgcagcgc caccgacgcg cgggcacgcg
     1981 catcacgctg cgcctgcgcg cggacgaggc gcgcgcgacg ctggagctgc acaacgaagg
     2041 cgagcccatc ccgcccgagc tgatcgacag gatcttcgag tacggcgtct cgggcacgca
     2101 ggacgacgag ggcgcgggcg tggacgccga ccgccacgcg ccgcagcgcg gccagggcct
     2161 gttcgtcgcc aagacctaca tggccaagat gggcggcacc atcgccgccg agaacgtccc
     2221 cggcggcgtg cgcttcgtgt tgacgctgcc gcgcgcctga acgaccgccc cgaacggggt
     2281 caggtcttga atgttgcaac gttatcacgc cgcgtgcggg caatgcagca tcgcggccgc
     2341 caggcgatgc gatcatcgcg ccatgcgccg ccacctctgc ccacgcgcct tcgccgccgc
     2401 cctcgcgttc gccacgctgc tgcccgcgtt cgctcagtcg gacgcacccg ccacgacgcc
     2461 gcgccgcttc gaacggctgc gccaggcgat ggccggcgac ctggaggacg gcagcgcgca
     2521 ggaccgctcg gcccgcggcg cgccgagcgg cacgcggctg gtccccgaca tcccgtacgg
     2581 ccccgatccc gcgcagcgcc tggacgtcta cgtgtcggcg cagccgcagg ccggcaccgc
     2641 gccgctgccg gtcatcttct tcgtgcacgg cggcgcctgg gcgcgcgggg acaagacgaa
     2701 cacgcgcgtc gtcgagccca agatcgcgca ctgggtcgac cagggcttcg tggtgatctc
     2761 cgccaactac cgcatgctgc cgacgccggt ggcgcagcag gccgacgacg tcgcggccgc
     2821 gatcgcgttc gcgcagtcgc aggccggcct gtggggcggc gacggcaggc gcttcatcct
     2881 gatgggccac tcggcgggcg cgcacctggt cgcgctgatc gccgccggcg cgcgcacggc
     2941 gacccggccg caaccctggc gcggcgcggt gctcctggac agcgccgcgt tcgacgtgac
     3001 gacgctgatg acgcaccgcc acttcggcct ctacgaccac gcgttcggca gcgaccgcgc
     3061 ctactgggcc gcgatgtcgc cgatcgagca gctcgcgcat gcgacggcgc cgatcctggc
     3121 ggtgtgctcg tcgcgccgcc aggagtcctg cggccaggcc gatcgcttcg cgggcaaggc
     3181 gaacggcctg ggcggcaagg tgcgcgtgct gcgcgaggac ctgtcgcaca aggagatcaa
     3241 cgcgacgctg ggcgccgcgt cggactacac gtcgcaggtc gacgcgttcc tcgcgtccgt
     3301 gcgctgagcg acctcaggcc gccgcgagcg cgaggttcgc ccgccgcagc cccgcgatcg
     3361 tcatcaggca ggcctgcgcg acctcgacgc ccttcttgac gaagtgctcg gcgaagaagc
     3421 gcctgtgctc gtcgtgctcg tggaagtcgc gcggcgtcag caccgccgac atcaccggca
     3481 cgccggtatc gagctgcacg cgcatcaggc cgtcgatgac cgccgagccg acgaactcgt
     3541 ggcggtagat gccgccgttg accaccagcg cgcaggcgac gatggcgtcg tagcggcccg
     3601 agcgcgcgag cgtctgcgcg agcagcggga tctcgaaggc gccgggcacc tcgtactggt
     3661 cgaccttgcc cggcggcgtc ggacttcggt cgaactcgcc gagcagcgac tgcttcgcgc
     3721 gatcgacgat gtcggtgtgc caggaggcgc agatcaccgc gatgcgggcc cccgcgctgg
     3781 tcgcggtggc cgccgaggcg gcattggaaa gatggggagt catctgattc atggtttgct
     3841 cacggtcgag tggttgccag aatcagggcg cgcgagaacg cagcggccgc acggccggtt
     3901 tcaagcggac atgcggcagc tcacacttcg cgtttctctt tcatccggac tgtgaccgtc
     3961 ggccctggca tcgcaccagg tctgctgacc ctccgctcgt gaagacgcga aggcgctcgc
     4021 gggctcccgc gacgagtcgc ggataccgcc ggtggggaat ttcgccccgc cctgagaacg
     4081 ctgtcggcct tgcgggccga catgcccatt ctagggaggg cgtcgcaacg tcggtgtcgc
     4141 gagggttact gaccctggct cacgggagcc acgccgccag gtccttcttc agcttggcca
     4201 ggtcctcgtc gcgcgtgtac atcatgtggc ctgcgtcgta gtactggtgc gtgacgcgcc
     4261 ggcgcacgtc ggcggggatg tccaactgcg cgagcgacca gtcgctggcg gtcgacggcg
     4321 tgcccaggtc gaagtggccg ctggccacca gcacgcgcag gtgcgggttg cggcgcaggg
     4381 cgcgcgacag gtcggggctg gtgcagcaga acgagttgcc ctgcacgtcg ccgcggctcc
     4441 agttccagtg cttgttggtg tccatggaca gcacctcgta gggccaggac gggtcgaacc
     4501 cgagcgtgcc gatgaagtag cccatgccgc ccatcgtgta gggcccgacc agcgattcca
     4561 tgcccgggtc gaagtcccac tcgtgcgttc gcgacagccc catcggcccc gcgacgcgcg
     4621 cctcgagccg gcccacgatc tggccgcgat cgcgcaagag ctcggtgaag aaggtgttgt
     4681 ccgagatgcg caggttgcgg tgctcgacca gcgtctgcga caggcccgtg agctccgcca
     4741 tccgcctggc gaccttcgcg cgctcgtcgt cggtggcgcg cgcgcccttg tgcagcacgc
     4801 gcaggtagtc ggactcgacg aagtccgtgg ccagcgcgcg cgccgcggcg gccgacttcg
     4861 atggcggccc cttgaggcag ccatggtact gcgcgacgcc ggcgaacgcg ggcaggaaca
     4921 gcgcgtacgg caggtcgttg cgcgcctcga agacgatgga ttgcaggtcc atcgcgcacg
     4981 agatcaggat cagcccggcg agcgacacgc cctggtcggc cagcttgtcg gcgatggccg
     5041 cgccgcgcgt cgtgccatag ctctcgccgg ccacgtagat cggcgacgtc cagcgctggt
     5101 ggcgcgacag ccaggtgcgg atcacgtcgg tcaggcaggc gacatcgccg tcgaccgaca
     5161 gcatcttctt gcgcgcgtcg tcgctggcgg tgatcgagta gcccgtgtgc ggcgggtcga
     5221 cgaacacgag gtcgaagtgc tcgaaccagc tgagcgggtt gtcctcgacc tcgtagggcg
     5281 gcggcagcat cgagccgtcg ttgccgatgc gcaggcgctt gggccccatc gcgcccagct
     5341 gcagccagat cgaggccgag cccggccccc cgttgaacgc gaagcagacg ggccgcggcc
     5401 tggtgtcgcc ggccttgcga tcgagctggt aggcggtggt gaagacggcg gcctcgggct
     5461 cgccgcgctt gtcggtctgg gtcgcgttga cgaccgggac gaacgcgccc tcgaccttgt
     5521 actcgagctt cctgccaccc agcttgatcg agccggtgct gcgcatgggt gcggtggcca
     5581 gcagcttctc cacggcgtcg cgctgcgtct gcctggcctt gtcggcgtcg ggcttggcgt
     5641 cggcggcggt cttcggttcg gtcatgggct ccctttgcac ggtgtccggg tcggcgcatt
     5701 gtggcgcgcc ggacgcgccc gcgggcccgc tcccgatcag ggacttcgcg gagtcaggcc
     5761 gccgccggtc cctggaacgc gccggcgcgg aaggtcagca ccagcgtgtc gcgccagccc
     5821 ggctcgcccg acctgagcgg ctggatcggc gtggtctcgt ggatgacgcg ggcgtcgtcc
     5881 agcagcaccg tcgtccacgg ctgcgacagc gtgaagcgca ggccggcgct ggatgccgcc
     5941 tggaacacgc ggctctcgcc gcccttgatg ccgtggcggt cgaccagcgt cacgctgacg
     6001 aggtcgacgc cgtcgcggtg cgcgccctcg ggcgtcggcc ggccgatgcc gctcgcggtg
     6061 tcgatgcgga acgtgtgcgc ctcgacgaac cagcgttgcg cgcccttcag ggccgaggcg
     6121 cgcgccgcca ggccggccag cagccggcgc cacgcggggt cgcccgccag gccggcctcg
     6181 atcggctcga aatggcgttc gatgccgccg tgcagcgcgt tgtagtcgac cggctgccag
     6241 tgcgggcgat gcggcgccgc gctcaccgtg tcgccgtcga cgacgaacac gccgtggcgg
     6301 cgctggcggt acttgccgcc atcgagcagg taggcgtccg cgggcaggcg gttccagaac
     6361 ggccgccagg cgtcgaagtc cgccggggtc gcgccggcca gcgccgccag cccctccggc
     6421 cccacgacgg cgaagccggc gcccgcgagg gcggcgtcga cgtcgccggc aggcgtcagc
     6481 gggggcggga tcaggctgtt cgggtcggac atgacggaag gcgacggggg cgtcgctgga
     6541 cagggggtct ggcacattct cgcgcgtgcc gccatccccg cgtccggtca ggtggcggga
     6601 agcgacagcg gccgccctgc ccgacaggtg gagcgtagac tgacgagtct ccccgatcca
     6661 tcgccacgcc atgtccgcct ccaagggcca ctccagaccg agcgtcgtgc ccgcggcccc
     6721 cggcccggac gatggcgccg cgccggccgc cctgcccgcg gccctgctgg cgtggggccg
     6781 cacggcaccg gtggccctgg cggcgatcgc ggccgacggc acggtcgcct gggtcaaccc
     6841 ggcgttcgag cgtctcgccg ggctgctgtc ggagcaggcc acgggccagc ccctggaaac
     6901 cctgctcgcc gacgccaact ggccgcgctg gctgccgccg cccggcacgc ttgcgcggcg
     6961 ccgcctgctg cggcgcgatg cgacggcgct gcactgcgac gcgcaggtcg acgcgatcga
     7021 gggcggcctg cgcctggtga cgctcgtcgg ccgcgacgac gaggcccgca tcgacctcga
     7081 ggcatgccgg ctcaaggccc tgctggactt cgtccagacc cacgcgcgca tcggcctgtg
     7141 ggagcgcaac gtgcgcacgc gcgagggcaa gtgggacccg cacatgtacc gattcttcgg
     7201 catcgacccg cgccacgggc cgccgtcgat cgcgcagatc gcgcaggcca cggtgccgca
     7261 ggaccgcctg ttccaggtgc tggacgactc gatgaggaag gcgggcagct actcgcaccg
     7321 ctaccgcctg ttcaccgccg acggcggcct gcggcgcgtg caggcgcact gggaggtg
//
LOCUS       DATCRO010000522         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_827114, whole genome shotgun sequence.
ACCESSION   DATCRO010000522 DATCRO010000000
VERSION     DATCRO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827114"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1010
                     /gene="dhaK"
                     /locus_tag="VIN75_14320"
     CDS             27..1010
                     /gene="dhaK"
                     /locus_tag="VIN75_14320"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY8975389.1"
                     /translation="MKKFVNDIDDVMRESLSGLVSAHPDLIRVEFEPTFIARRNRTPG
                     KVALISGGGSGHEPLHAGFVGKGMLDAACPGQVFTSPTPDQMLAAAAAVDGGKGVLFI
                     VKNYAGDFMNFQMASEMLDIENATVLVNDDVAVENSTHTTGRRGVAGTVIVEKIVGAA
                     AELGATLAECKQVGDRVNKSTASMGLAFTSCTVPAAGRTTFDIGDDEMEVGVGIHGEP
                     GRRREKIKPADAIVDELLAAILDDLKPQHGRELILHVNGFGGTPLMELYLLHHSALSR
                     LRSAGLAPVRSLVGNYTTSLEMAGASLTVTLLDDELRGLWDAPVHTSAMRW"
     gene            1021..2169
                     /locus_tag="VIN75_14325"
     CDS             1021..2169
                     /locus_tag="VIN75_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975390.1"
                     /translation="MRRRQALAGTAAAAASWALAPGRALAAVADRSPIRLGQSAPISG
                     PFARQGLAFRDAARAVLDDTNAQGGIGGRRIELVTMDDENSPERTAVNVKLLASEQRV
                     SALFGFVGAGAHRAGPGGAREEGLPYVAPVSGSQEIRSGAFPWVFNVRAGHVDEIRFI
                     ARHTKLIGIERIALLTEYNSQGWELRDALMAAAESQGRKVASISSVDHEGGSYSLTGA
                     VSSVLAGRPEAVLVGADYVASARFVDAVRRGGFNGLFYTLSTVGGDALMERLGPLAVG
                     VSVTQVMPFPWSFASPVGRHFQAFCQRHLLQPSFPAMEAYVSTSLLMDALHGARGFAP
                     AQIAEALAMQAPRDLGGFVAAFTAGEGDRRAPPQVDLTVFSRGGKFIQ"
     gene            2166..2960
                     /gene="tpiA"
                     /locus_tag="VIN75_14330"
     CDS             2166..2960
                     /gene="tpiA"
                     /locus_tag="VIN75_14330"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8975391.1"
                     /translation="MTSAKLVVGNWKMTGDVARVQVFAGQAAGELSALGTRCEAAICV
                     PHPLLHVAQSAWWCTPLRWGAQDCSAVDDGKSTGDVSAGMLRAAGARYVIVGHSERRH
                     GRGETDADVSAKARRALDNAVTPIVCIGETAAERDDHRAEEVLRRQLQHLVATLGGSL
                     CSIVLAYEPVWAIGTGRTASVGMIEDVHGFIEHVLRFHGQCDVRSVRRLYGGSVDPRN
                     ARAIMACPGVDGVLVGGASQRAADFLDICKAATGGTAGTTHGSEDG"
BASE COUNT          438 a         1052 c         1158 g          448 t
ORIGIN      
        1 caacctgatc tgaagcagga ggccgtatga agaagttcgt caacgacatc gatgacgtga
       61 tgcgcgagtc cctgtcgggc ctcgtgtcgg cccacccgga cctgattcgc gtcgaattcg
      121 aacccacgtt catcgcgcgc cggaacagga cacccggcaa ggtcgcgctg atctccggtg
      181 gcggctcggg gcacgagccc ctgcacgccg gcttcgtcgg caaggggatg ctcgacgcgg
      241 cgtgccccgg ccaggtgttc acctcgccga cgcccgacca gatgctcgcg gccgccgcgg
      301 ccgtcgacgg cggcaagggc gtgctgttca tcgtcaagaa ctacgccggc gacttcatga
      361 acttccagat ggcgtcggag atgctcgaca tcgagaacgc cacggtgctg gtcaacgacg
      421 acgtcgcggt cgagaactcg acgcacacga ccggccggcg cggcgtcgcg ggcacggtga
      481 tcgtcgagaa gatcgtcggc gccgctgcgg agctgggcgc cacgctcgcc gagtgcaagc
      541 aggtgggcga ccgcgtcaac aagtcgaccg cgtcgatggg cctggcgttc accagctgca
      601 cggtgccggc cgccggccgc acgacgttcg acatcggcga cgacgagatg gaggtcggcg
      661 tcggcatcca cggcgaaccg gggcgccggc gcgagaagat caagccggcc gacgcgatcg
      721 tcgacgagct gctcgccgcg atcctggacg acctcaagcc ccagcacggg cgcgagctca
      781 tcctgcacgt caacggcttc ggcggcacgc cgctgatgga gctgtacctg ctgcaccaca
      841 gcgcgctgtc gcgcctgcgt tcggccggcc tggcgcccgt gcgctcgctg gtgggcaact
      901 acacgacctc gctggagatg gccggcgcct ccctgaccgt gaccctgctg gacgacgagc
      961 tgcggggcct gtgggacgca ccggtgcata cctcggcgat gcgctggtag ggagacggcc
     1021 atgcgcaggc gacaagctct tgccgggacc gccgccgcag cggcgtcgtg ggccctcgcg
     1081 ccgggccgtg cgctggctgc cgtcgcggat cgctcgccga tccgcctggg ccagtccgcg
     1141 ccgatcagcg gcccgttcgc gcgccagggg cttgcgttcc gcgacgccgc gcgcgcggtg
     1201 ctcgatgaca cgaacgcgca gggcggcatc ggcggccggc gcatcgagct ggtgacgatg
     1261 gacgacgaga acagccccga gcgcacggcc gtcaacgtca agctgctcgc ctccgagcag
     1321 cgcgtgtcgg cgctgttcgg cttcgtcggc gccggcgccc atcgcgctgg cccgggcggc
     1381 gcgcgcgagg aggggctgcc ctacgtcgcg ccggtgagcg gatcgcagga aatacgctcg
     1441 ggcgcgttcc cgtgggtgtt caacgtgcgt gccggccacg tcgacgagat ccgcttcatc
     1501 gcgcggcata cgaaactcat cggcatcgag cgcatcgcgc tgctgaccga atacaactcg
     1561 cagggctggg agctgcgcga cgcgctgatg gcggcggccg aatcgcaggg gcgcaaggtc
     1621 gcttcgatct cgagcgtcga ccacgaaggc ggcagctaca gcctgaccgg cgccgtgtcg
     1681 tcggtgctgg ccggccgacc cgaggcggtg ctcgtgggcg cggactatgt cgcctccgcc
     1741 cgcttcgtcg acgccgtgcg ccgcggcggc ttcaacgggc tgttctacac gctgtcgacc
     1801 gtcggtggcg atgcgctgat ggagcgcctc gggccgctgg ccgtgggcgt gtcggtcacg
     1861 caggtgatgc cgttcccgtg gtcgttcgcc tcgcccgtgg gccggcattt ccaggcgttc
     1921 tgccagcgcc acctgctgca gccgtccttc ccggcgatgg aggcctacgt ctcgaccagc
     1981 ctgctgatgg acgcgctgca cggcgcgcgc gggttcgcgc cggcgcagat cgccgaggcg
     2041 ctggccatgc aggcgccgcg cgacctgggc ggcttcgtgg ccgcgttcac cgcgggcgag
     2101 ggcgatcgcc gcgcgccgcc gcaggtcgac ctcaccgtgt tctcgcgcgg cgggaagttc
     2161 atccaatgac ctccgccaag ctcgtcgtgg gcaactggaa gatgacgggc gatgtcgccc
     2221 gcgtccaggt gttcgcgggg caggcggccg gcgagctgtc ggcgctgggg acgcggtgcg
     2281 aggcggcgat ctgcgtgccg catccgctgc tgcacgtggc ccagtcggcc tggtggtgca
     2341 cgccgctgcg ctggggcgcg caggactgct ccgcggtgga cgacggcaag tcgacgggcg
     2401 acgtcagcgc cgggatgctg cgggccgcgg gcgcgcgcta cgtgatcgtc gggcattccg
     2461 agcggcggca cggacgcggc gagaccgatg cggacgtgtc ggccaaggcg cggcgcgcgc
     2521 tggacaacgc cgtcaccccg atcgtgtgca tcggcgagac ggccgcggaa cgcgacgacc
     2581 atcgcgccga ggaggtgctg cggcgccagc tccagcacct ggtcgcgacg ctgggcggca
     2641 gcctgtgctc gatcgtgctg gcctacgaac ccgtctgggc catcggcacc ggacggacgg
     2701 catcggtggg aatgatcgag gacgtgcacg gcttcatcga gcacgtcctg cgctttcacg
     2761 ggcaatgcga cgtgcgttcc gtgcgtcgct tgtatggcgg gagtgtggat ccgcgcaacg
     2821 cgagggccat catggcctgt ccgggcgtcg acggcgtgct cgtcggcggg gcgtcgcagc
     2881 gcgcggccga tttcctcgac atctgcaagg cggcgacggg cggtaccgcg ggcacgaccc
     2941 acggcagcga agacggctga gcctgcatct cgctggaggc gggtatgtgc aagacggcaa
     3001 cgggcgaaga gctgagggcg ctgcgccttc aggtgagtga gctgcgtcac gcgaaggagc
     3061 aggccgaggc cggcacgcgg gcgcactgga tgctgg
//
LOCUS       DATCRO010000523         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_39439, whole genome shotgun sequence.
ACCESSION   DATCRO010000523 DATCRO010000000
VERSION     DATCRO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39439"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VIN75_14335"
     CDS             <1..1189
                     /locus_tag="VIN75_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975392.1"
                     /translation="QMALSLRDQGEIAPEDARALADGQLDYVVQIWREDGHEVYATQL
                     QNEPPARAVLGFADISAGGVVWRTFGVETRDRIIQVAQPERVRHERAAQAALRSVVPL
                     LAVAPALALVIGWLVSRSLAPLQRLAAEVRSRDSSSLQALPEGDLPDEVLPLVQSLNA
                     LLDRLGAALAAQRHFVGDAAHELRSPLTALKLQAQVLRRAPDEATRDEAARALTAGVD
                     RATRLVEQLLALARSEAGPPREASTAPLAELVRQAMGDVAPFADARGTTLELDVADGA
                     EAATVSGEGDVAVLARNLIDNAVRYSPAGGHVSVWVGTDAERAGAPVLQVDDDGPGIP
                     AADREIVFDRFVRRDSAAGQTGSGLGLAIVRQVALRQGADVVLGVSPAGGLRVRVRWP
                     ARA"
     gene            1361..2923
                     /locus_tag="VIN75_14340"
     CDS             1361..2923
                     /locus_tag="VIN75_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY8975393.1"
                     /translation="MNKTNLKPLAWALLGAGVAVMGTAGALDLPSWLRHSSSNAVVTP
                     GAGGGTAGIAAAATGQQVAPTPITPPLPALPPGTAPNYRAIVQEFGPAVVHVSVEGTH
                     KPTNDEIQEQGGMSPDDPLFRFFRGMPGMPNFQRGGPGLEQPFKGLGSGFIIGSDGLI
                     LTNAHVVKEAKDVTVKLQDRREYVAKVLGVDAATDIAVLKIDAKGLPTVRFGQPSKLM
                     VGDYVLAIGAPYGLDQTATSGIISAKGRSLPSEGGENFVPFLQTDAAVNPGNSGGPLF
                     DAAGNVIGINSQIYSRTGGFQGVSFAIPIDVALAIKDQIVATGHATHARLGVSIQPLT
                     QDLAESFGLERPDGALVATVAPNSGAAKAGLRSGDVITKVNGQPIEEPGQLSSRIGMS
                     RPGEKVTLEVWRDKKLETVVATLGASTDKLDVASKGSDGGGSEKLGLALRPLNRDEKG
                     EVGSSGLVVENADGAAAKAGVEQGDVLLAVNGKPVGSVEEVKSILATKPKRVALLIWR
                     DGSRIFFPVNLG"
     gene            complement(3221..3415)
                     /locus_tag="VIN75_14345"
     CDS             complement(3221..3415)
                     /locus_tag="VIN75_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HEY8975394.1"
                     /translation="MKKILIAVLSLAIGATAFAQSGAPAPAAQTATASPDTAASAVAK
                     KKAKRAAKKAAKKAKAASAA"
BASE COUNT          539 a         1364 c         1401 g          517 t
ORIGIN      
        1 gcagatggcg ctgtcgctgc gcgaccaggg cgagatcgcg cccgaggatg cgcgcgcgct
       61 ggccgacggg cagctcgact acgtggtgca gatctggcgc gaggacgggc acgaggtcta
      121 cgcgacgcag ctgcagaacg aaccgcccgc gcgcgccgtg ctgggctttg ccgacatctc
      181 cgccggcggc gtcgtctggc gcacattcgg cgtcgagacg cgcgaccgca tcatccaggt
      241 ggcgcagccc gagcgcgtgc gccacgagcg cgccgcgcag gccgcgctgc gcagcgtggt
      301 gccgctgctg gccgtcgcgc cggcgctggc gctggtgatc ggctggctgg tctcgcgctc
      361 gctcgcgccg ctgcagcgcc tggccgccga ggtgcgctcg cgcgacagca gctcgctgca
      421 ggccctgccc gagggcgacc tgcccgacga ggtgctgccg ctggtgcagt cgctcaacgc
      481 gctgctcgac cgcctcggcg cggcgctggc cgcgcagcgc cacttcgtcg gcgacgcagc
      541 gcacgagctg cgctcgccgc tgaccgcgct gaagctgcag gcgcaggtgc tgcgccgcgc
      601 gcccgacgag gccacgcgcg acgaggccgc gcgcgcgctc accgcgggcg tcgatcgcgc
      661 gacgcgcctg gtcgagcagc tgctcgcgct cgcccgcagc gaggccgggc cgccgcgcga
      721 ggccagcacc gcgccgctgg ccgaactcgt gcgccaggcg atgggcgacg tcgcgccctt
      781 cgccgatgcc cgcggcacga cgctggagct ggacgtggcc gacggcgccg aggccgcgac
      841 cgtctcgggc gagggcgacg tcgccgtgct ggctcgcaac ctcatcgaca acgcggtgcg
      901 ctacagcccg gcgggcggcc acgtgtcggt gtgggtgggc acagacgcgg agcgcgcggg
      961 ggcgccggtg ctgcaggtcg acgacgacgg ccccggcatc ccggccgccg atcgcgagat
     1021 cgtgttcgac cgcttcgtgc ggcgcgacag cgcggccggc cagacgggct ccggcctggg
     1081 cctggcgatc gtgcgccagg tcgcgctgcg ccagggcgcc gacgtcgtgc tgggcgtctc
     1141 gcccgcgggc ggcctgcgcg tgcgcgtgcg ctggcccgcg cgcgcctgac gcgcgaccgc
     1201 caaggcctcc gcggcgtccg ccgcgtccta atgttcgcct catgttgagc taatggagcc
     1261 ttcatccatg gcgcgcacat tgccaccatg cgccgcgaag gcccgggttt cccggaaccg
     1321 gcgcgccgtc tcgaatgaag ccgacaagga gttcgatctg atgaacaaga ccaacctgaa
     1381 gccgctcgcg tgggccctgc tcggcgcggg cgtggcggtg atgggcaccg ccggcgcact
     1441 ggacctgccg tcctggctgc gccattccag cagcaacgcc gtggtcaccc cgggcgccgg
     1501 cggcggcacc gcgggcatcg ccgcggcagc caccggccag caggtcgcac cgacgccgat
     1561 cacgcccccg ctgcccgcgc tgccgccggg cacggcgccg aactaccgcg ccatcgtgca
     1621 ggagttcggc cccgcggtcg tgcacgtcag cgtcgaaggc acgcacaagc cgaccaacga
     1681 cgagatccag gaacagggcg gcatgagccc ggacgatccg ctgttccgct tcttccgcgg
     1741 catgcccggc atgcccaact tccagcgcgg cggccccggc ctggagcagc cgttcaaggg
     1801 cctgggctcg ggcttcatca tcggctcgga cggcctgatc ctgaccaacg cgcacgtcgt
     1861 caaggaagcc aaggacgtca ccgtcaagct gcaggaccgc cgcgagtacg tggccaaggt
     1921 gctgggcgtg gacgccgcca ccgacatcgc cgtgctcaag atcgacgcca agggcctgcc
     1981 cacggtgcgc ttcggccagc cgtccaagct gatggtgggc gactacgtgc tggccatcgg
     2041 cgcgccctac ggcctggacc agacggccac ctccggcatc atcagcgcca agggccgttc
     2101 tctgcccagc gagggtggcg agaacttcgt cccgttcctg cagaccgacg cggcggtcaa
     2161 ccccggcaac tcgggcggcc cgctgttcga tgcggccggc aacgtgatcg gcatcaactc
     2221 gcagatctac agccgcaccg gcgggttcca gggcgtgagc ttcgcgatcc cgatcgacgt
     2281 ggcgctcgcg atcaaggacc agatcgtcgc caccggccac gcgacgcacg cgcgcctggg
     2341 cgtgtcgatc cagccgctga cgcaggacct ggccgagagc ttcggcctgg agcgccccga
     2401 cggcgcgctg gtggccaccg tggcccccaa cagcggcgcg gccaaggccg gcctgcgctc
     2461 gggcgacgtc atcaccaagg tcaacggcca gcccatcgag gagccgggcc agctgtcctc
     2521 ccgcatcggc atgtcgcgcc cgggcgaaaa ggtcacgctg gaggtgtggc gcgacaagaa
     2581 gctcgagacg gtcgtcgcca cgctgggcgc cagcaccgac aagctcgatg tcgcctccaa
     2641 gggcagcgac ggcggcggca gcgagaagct gggtctggcg ctgcgcccgc tcaatcgcga
     2701 cgagaagggc gaggtcggct cgtccggcct ggtggtggag aacgccgacg gcgccgccgc
     2761 caaggccggc gtggagcagg gtgacgtgct gctggccgtc aacggcaagc cggtgggctc
     2821 ggtcgaggag gtcaagtcca tcctggcgac caagcccaag cgcgtggcgc tgctgatctg
     2881 gcgcgacggc tcgcgcatct tcttcccggt gaacctgggc tgaggacggt cgcgaggcgc
     2941 gtcctcgcgg cgaatcgctg gaagagggcg ctcggcgaga gcgccctttt tcattggcct
     3001 gacttggtcg tcatcctgcg cgcagtcgca ggatccaccg tggccggccg ggcggcatca
     3061 tggcgggcgt ggcgtggatc ctgcgactgc gcgcaggatg acggctggtt ggcgtcccgg
     3121 ctgcgcgacc ccggccgggc ccgcccccga aaaaaaagcc gacgcggtcg cccggtcggc
     3181 tttcttcgtt ccagctcgcg agaaccgcga gcgcgtggaa tcaggcggcg gaagcggcct
     3241 tggccttctt ggcagccttc ttggcggcgc gcttggcctt cttcttggcg acggccgacg
     3301 cggcggtgtc gggcgaagcg gtggcggtct gggcggccgg ggccggcgcg ccggactggg
     3361 cgaaagcggt ggcgccgatg gcgagcgaca ggacggcgat caggatcttc ttcatggtgg
     3421 aaactttcag gttagggttt gtggcgtcgg ccgccacaag tgagcggggg acgccggaac
     3481 aacgcgcccc ttgcacgaac ggtttacgtg cctcccttct ttttcttgca tcgggatgaa
     3541 gaaaacgccg gaacgctcga ccggaaacgt ggcgggcgag attttcgagc agggccgacg
     3601 ggcccgcaac gccgccggcg cacagtctcc ctacgttgtg cgcgaaacct gaatccaagc
     3661 ttgcgctccg ctggtgccgc gcaccagggc ggggcgctgg cccgggaggc gctggccgcc
     3721 gacggcatcg gtccgattga cgcccagggc gcggggcgcg gaacgggccg tttcgcgtcg
     3781 atcggcgccc gaaagtccaa ttgcgcgccg tctatcacac c
//
LOCUS       DATCRO010000524         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_287008, whole genome shotgun sequence.
ACCESSION   DATCRO010000524 DATCRO010000000
VERSION     DATCRO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287008"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VIN75_14350"
     CDS             complement(<1..208)
                     /locus_tag="VIN75_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975395.1"
                     /translation="MTSLVESWKVLPNGPLTAVDDHLSMSLSLDDLPDLPGAREAALD
                     DAPDALPAQLLEWSEDFELRGIHPC"
     gene            complement(378..1097)
                     /locus_tag="VIN75_14355"
     CDS             complement(378..1097)
                     /locus_tag="VIN75_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975396.1"
                     /translation="MPAILDHWKVLPHGPLTAIDDKVWTVTGTIHMPVGDFERRMSVV
                     RLRDGRLVIWSAIALDEPEMARLEAFGRPAWLVVPGDDHRMDAKIWLQRYPHLDVATP
                     AGARDKVLEAVPVDSSAPDFQDPDVQWITVPGTQGHEAALTVRGSKGMTLIVNDLIAN
                     LRHPHGFGGWMLKLMGFAGDEPNIPAVRRMAMIKDSQALADQLQAWSDLPELHCILVS
                     HGEPIVDDPRGELQRLADKLR"
     gene            complement(1149..2441)
                     /locus_tag="VIN75_14360"
     CDS             complement(1149..2441)
                     /locus_tag="VIN75_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975397.1"
                     /translation="MSLHLPGRRARARLARRREARRALRQPSGFRIAIADAISQLDPA
                     AWDEVVADESWFFSREYLAMLERVPPSVVEPRYALVCDAQGPVAAVVLQWAEVDGTRL
                     RPLPAGAGGDDELNPLRRLVDKLSRPARRAFATRLRERVLVCGNLLTYGQHAVAVAPD
                     VDPDGVWPAVAEVLYRIRRAEKLAGQAGFVLIKDIPLAANAGVARLADVGYKGIETEP
                     NMVLALDAAWKTHADYLGGLTSKYRSAVRNQILQPIEQAGLAVRAYVPSGALARRTHE
                     LYLGVHRNAALRPFTLHEDYFAELAAAAGARARFAGLFDVPDDTPEALLGFIVTLADV
                     DEALAYHIGFVQDDPRGLPLYLRLLHASIADAIALEATALSFGRTALEPKARLGARPQ
                     DMQVWLRHRQPVFNRIVRQLTGFAHHAEAPETHPFKKS"
     gene            complement(2575..3204)
                     /locus_tag="VIN75_14365"
     CDS             complement(2575..3204)
                     /locus_tag="VIN75_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8975398.1"
                     /translation="MASRQRKPPARGYHHGDLKNALRDAASALIAERGVEAVSLREIS
                     QAAGVSHTAAYRHYADKQALLAALAEEGFRQLADINRRTIAETPGGPVPQLMACGRAY
                     VRFGVQRPHVLQLMFSQAIPDWSAHPSLQQASEELAQTLAGVVAAGQSAGAMQPAPLG
                     EMTLTAWSLVHGLALLLAGRRIPAPAIDDAFVEHMAEQCVALLMRGLAR"
     gene            3279..3734
                     /locus_tag="VIN75_14370"
     CDS             3279..3734
                     /locus_tag="VIN75_14370"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY8975399.1"
                     /translation="MATDLLLACLHHLVVFSLVTIPAVELALMRGNVGATRIAHLARL
                     DAVYGLLAMAILAIGFGRVYWGAKGPAFYLHNPVFWAKLAFFALAGLASAAPTVRILR
                     WKKAVAREPGFAAPDTDVASTRRWLAAELALFVPIPLLAAAMARAVGLG"
     gene            3747..4094
                     /locus_tag="VIN75_14375"
     CDS             3747..4094
                     /locus_tag="VIN75_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY8975400.1"
                     /translation="MTTLYGIPNCDTVKRARDWLATHGVAHEFHDLKKAGVPADRLQA
                     WVDAAGWERVLNRKGTTWRKLDPSLQASVVDAKTAQALMREQASVIKRPVVEWDDGRI
                     TVGFDADDWETRA"
BASE COUNT          626 a         1586 c         1672 g          639 t
ORIGIN      
        1 cgcaagggtg gatgccgcgc agctcgaagt cctcgctcca ctcgagcagc tgcgcgggca
       61 gggcatccgg cgcgtcgtcc agggccgcct cgcgcgcgcc cggcaggtcc ggcaggtcgt
      121 cgagcgacag ggacatcgac aggtgatcgt cgacggccgt cagcgggccg ttcggcaaga
      181 ctttccagga ttcgactagc gaggtcatcg cggactccgg ttcgaccgca cggcgcggcc
      241 agcgcgggag cccgccctgg gctggccggg ctccgacaga cccggtccgg cggcaagcgc
      301 catgccttgc cacggtccgc catgactgcg ggcgcgacag ggcgccgccg tcgtcgcgac
      361 ccgtcgaggc ggcggcgtca gcgcaacttg tcggccaggc gctgcagctc gccgcgcggg
      421 tcgtcgacga tgggctcgcc gtgcgacacg aggatgcagt gcagctcggg caggtcgctc
      481 caggcctgca gctggtcggc cagcgcctgg ctgtccttga tcatcgccat gcggcgcacg
      541 gcggggatgt tcggctcgtc gccggcaaag cccatcagct tgagcatcca gccgccgaag
      601 ccgtgcgggt ggcgcaggtt ggcgatcagg tcgttgacga tcagcgtcat gcccttggag
      661 ccgcgcaccg tcagcgcggc ctcgtggccc tgcgtgcccg gcacggtgat ccactgcacg
      721 tcgggatcct ggaagtcggg cgccgacgag tcgaccggca ccgcctccag caccttgtcg
      781 cgcgcgccgg ccggcgtggc gacgtccagg tgcgggtagc gctgcagcca gatcttcgcg
      841 tccatgcgat ggtcgtcgcc gggcacgacc agccaggccg ggcggccgaa ggcctccagg
      901 cgcgccatct cgggctcgtc gagcgcgatg gcgctccaga tcaccagccg gccgtcacgc
      961 agtcgcacga cgctcatgcg gcgctcgaag tcgcccaccg gcatgtggat cgtgccggtg
     1021 accgtccaga ccttgtcgtc gatggccgtc agcggcccgt ggggcaggac cttccagtgg
     1081 tcgaggatcg cgggcatcgg gcgctcccgt tcagctgcag tgccctcgat cggcaaggca
     1141 catgccgctc agctcttctt gaacgggtgg gtctccggcg cctcggcatg atgcgcgaag
     1201 cccgtgagct ggcgcacgat gcggttgaac accggctggc gatggcgcag ccacacctgc
     1261 atgtcctgcg gcctggcgcc cagccgcgcc ttgggctcga gcgcggtgcg gccgaacgac
     1321 agcgcggtcg cctccagcgc gatggcgtcg gcgatgctgg cgtgcagcag gcgcaggtac
     1381 agcggcaggc cgcgcggatc gtcctgcacg aagccgatgt ggtacgcgag cgcctcgtcg
     1441 acgtcggcca gcgtcacgat gaagcccagc agcgcctcgg gcgtgtcgtc gggcacgtcg
     1501 aagaggcccg cgaagcgcgc gcgcgcgccg gcggcggcgg ccagctcggc gaagtagtcc
     1561 tcgtgcagcg tgaacggccg cagcgccgcg ttgcggtgca cgcccaggta gagttcgtgc
     1621 gtgcgccgcg cgagcgcgcc cgagggcacg tacgcgcgca cggcgagccc ggcctgctcg
     1681 atgggctgca ggatctggtt cctgaccgcg ctgcggtact tggacgtcag gccgccgagg
     1741 tagtcggcgt gcgtcttcca ggccgcgtcg agggccagca ccatgttggg ctcggtctcg
     1801 atgcccttgt agccgacgtc cgccagccgc gcgacgccgg cgttcgcggc cagcgggatg
     1861 tccttgatca gcacgaagcc cgcctgcccg gcgagcttct cggcgcgccg gatgcggtac
     1921 agcacctcgg cgacggcggg ccagacgccg tcggggtcga cgtcgggcgc gacggccacc
     1981 gcatgctggc cgtaggtcag caggttgccg cacaccagca cgcgctcgcg cagccgtgtc
     2041 gcgaacgcgc ggcgcgcggg gcgcgacagc ttgtcgacga ggcggcgcag cggattcagc
     2101 tcgtcgtccc cgcccgcgcc ggccggcagc gggcgcagcc gcgtgccgtc gacctcggcc
     2161 cattgcagga cgacagccgc cacggggccc tgcgcgtcgc agacgagcgc gtagcgcggc
     2221 tcgacgaccg agggcgggac gcgctccagc atcgccaggt actcgcggct gaagaaccag
     2281 gactcgtcgg cgacgacctc gtcccaggcg gctggatcga gctggctgat cgcgtcggcg
     2341 atcgcgatgc gaaagccgga gggctggcgc agcgcgcggc gcgcctcgcg ccggcgggcg
     2401 aggcgggcgc gcgcgcggcg gccgggcagg tgcagggaca tcggatctcc ttcggtggcg
     2461 cggcgtccgg tggctgccgc gggtcgacgg cgattctggc tcgccacggt cgagcagcac
     2521 gccgccgcgg cgaggtcgcg agaggcgata ccgcgcggtg cgtccggtgc gccgtcagcg
     2581 cgcgagaccg cgcatcagca gcgcgacgca ctgctcggcc atgtgctcga cgaaggcgtc
     2641 gtcgatggcg ggcgcgggaa tgcgccggcc cgccagcagc agcgccaggc cgtgcaccag
     2701 cgaccacgcc gtcagcgtca tctcgccgag cggtgcgggc tgcatcgcgc cggccgactg
     2761 gccggccgcc acgacgccgg ccagtgtctg cgcgagttcc tcgctggcct gctgcagcga
     2821 cggatgggcc gaccagtcgg ggatggcctg gctgaacatc agctgcagca cgtgcgggcg
     2881 ctgcaccccg aagcgcacgt aggcgcgacc gcaggccatc agctgcggca ccgggccgcc
     2941 gggggtctcg gcgatggtgc ggcggttgat gtcggcgagc tggcggaagc cttcctcggc
     3001 cagggcggcc agcagcgcct gcttgtcggc gtaatgacga taggccgcgg tgtggctgac
     3061 gccggccgcc tgcgagatct cgcgcaggct cacggcctcg acgccgcgct cggcgatcag
     3121 cgcggacgcc gcgtcgcgca gcgcgttctt caggtcgccg tggtggtagc cgcgcgcggg
     3181 cggcttgcgc tggcgagagg ccatggtgcg cgggaggaat gttgacagtg ggcacattgt
     3241 ctcatacgat cgatgtaccc actgtaaaca ttcatgccat ggccaccgac ctgctgctcg
     3301 cctgcctgca tcacctcgtc gtcttctcgc tggtgacgat ccccgccgtc gaactggcgc
     3361 tgatgcgcgg caacgtcggc gcgacccgca tcgcgcacct ggccaggctc gacgcggtct
     3421 acggcctgct ggcgatggcc atcctcgcca tcggcttcgg ccgcgtgtac tggggcgcca
     3481 agggcccggc gttctacctg cacaacccgg tcttctgggc caagctggcg ttcttcgcgc
     3541 tggccgggct cgcgtcggcg gcgccgacgg tgcgcatcct gcgctggaag aaggcggtgg
     3601 cgcgcgagcc cggtttcgcg gcgccggaca cggacgtcgc cagcacgcgg cgctggctcg
     3661 ccgccgaact cgcgctgttc gtgcccatcc cgctgctggc cgcggcgatg gcgcgcgcgg
     3721 tcggcctcgg gtgacacaat cgacgcatga cgaccctcta cggcatcccg aactgcgaca
     3781 ccgtcaagcg cgcccgcgac tggctcgcca cgcatggcgt cgcccacgaa ttccacgact
     3841 tgaagaaggc cggcgtgccc gccgaccgcc tgcaggcctg ggtcgacgcc gcgggctggg
     3901 agcgggtgct caatcgaaaa ggcaccacgt ggcgcaagct cgatccgtcg ctgcaggcct
     3961 cggtggtcga tgcgaagacg gcgcaggcgt tgatgcgcga gcaggcgagc gtcatcaagc
     4021 gtccggtggt cgaatgggac gatggccgca tcaccgtcgg cttcgatgcg gacgactggg
     4081 agacacgggc ctgaggttgc gcgccccgtc gccgcacgca gccccgggtt cgcggcgccg
     4141 atcaccgcag ggagcgacca tgcaacgaac ttcagtcccg gtccgttcgc acaagagccc
     4201 gtcgccgcgc ggtcgatggt gagccgtatg ccttccggtg tcaggtctcg aatcgttgcg
     4261 gtgtcaggtc ttgattcgaa cactgcacga ttcaagacct gacacccgat ggcgaacacc
     4321 ggaggtgagc gcctcacccc agcgccttct ccagcaccgt gaacgcgagg tcgtcagcgc
     4381 ggcgatgcgg cgaggtcggc gtcagtggac ggttcgaaca tggcggcgtg ccggcgtggc
     4441 gggaaacgcg gtcgatcgca ccacgccggc agcgggcatg ccggccgggc gacaatcggg
     4501 gcttcgtcca ccaggaactc ccc
//
LOCUS       DATCRO010000525         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_50959, whole genome shotgun sequence.
ACCESSION   DATCRO010000525 DATCRO010000000
VERSION     DATCRO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50959"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VIN75_14380"
     CDS             <1..636
                     /locus_tag="VIN75_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8975401.1"
                     /translation="NMILIPALPDFRARHPELQVDLGVSDRPIDLISEGVDCVIRGGE
                     LADTTLVARRIADLDWVTCASPVYLKARGVPKHPSELIARVHGSEDRARVPGHAIAGY
                     FSSLTGRAFPLEFHRGDEHLQIAGETVMAVNESTAHLSALLSGAGLGQTFRFAAAPHL
                     ASGALRTILGDWTRPRHPLYVVYPASRHLSAKLRLFVDWVAEVFAPFDDRP"
     gene            complement(637..1611)
                     /gene="tehA"
                     /locus_tag="VIN75_14385"
     CDS             complement(637..1611)
                     /gene="tehA"
                     /locus_tag="VIN75_14385"
                     /inference="COORDINATES: protein motif:HMM:NF008032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEY8975402.1"
                     /translation="MNASRWAVPASYFSMVLGLTGLGNGWRVAARLWGVPAWIGESVM
                     AVAVAVWAVLLVRFAIKWIAVRNEARAEAAHPILCCFIALVPVATMLAGIGVLPHAHG
                     PGTVLIVVGAAASLLFGLWRHGGQWRGGRDEKATTPVLYLPTVGANLVSAIAMSALGW
                     PSWGQLFFGAGVLAWIVQESIILSRLLTVDPLPPLLRPTVGIQMAPPAVALLAYAAVT
                     VGPPDLIARMLLGYAIVQALMVLRLMPWIRQPFTLSYWAFTFGATAISTGTMRFAERG
                     HDPVFTTLAPVLFAMSNLVIAGLSIASVMWALRAKPAAASAPVAPAAG"
     gene            complement(1769..3397)
                     /locus_tag="VIN75_14390"
     CDS             complement(1769..3397)
                     /locus_tag="VIN75_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8975403.1"
                     /translation="MGRAYSDITFTPTVRDVQSEKGSREQYAFLDTMSDRGDALTPRE
                     AQFLAEADHFFQATVSETGWPYVQHRGGPKGFLKVLDQRTLGFADFRGNVQYLSVGNL
                     RKDDRLSMIVVDYPNRRRLKLLGRVELVEAGVSAQGDAAIAAVSDPSYGATVERAFLI
                     RIEGWDWNCPQHITPRFTETEVASLTAPLRAQVQKLKSQLSEAKAALAASAAPSSASA
                     PQSLGDGPLALTISGVRQLTPSVRAFELRSADGSPLPTVIAGAHLDLPVRLADGTDST
                     RSYSIASSPHRTDAYEIAVQREADGRGGSVAVHEAFTLGLRLNASMPKHLFSLEETPH
                     RAVLVAGGIGVTPIKAMAHALIARGSEFELHYAVRSRTQAPFLGELIEAFGDRVKVHA
                     KDEGHRLDVEALMLGLDESDHVYVCGPSRLIDAVSAAATSAGVASARVHAEKFSADTT
                     RPGDREFVVHLVRSGKDIVIPKDRSILEVAEAQGVRVPASCRIGNCGTCATRVVAGTP
                     AHRDEVLTDAQKSEQQLMCVCVSRADGGELTLDL"
     gene            complement(3426..3977)
                     /locus_tag="VIN75_14395"
     CDS             complement(3426..3977)
                     /locus_tag="VIN75_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447949.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY8975404.1"
                     /translation="MSRIHVVESSTATPEQKALLDAIQGQLGMVPNFLKVFANSTTAL
                     QAFLGLYGVANGGSLDPLDRERIALALAQQNGCQYCVSAHTAIGRKTGLSAEEIAAAR
                     AGTSDDDTSAAILKFARSLMEKRGEVSALELTEVRAAGVDEAQIVEIITHVGMNFLTN
                     ILGKASLVEIDFPSVKLELPATA"
     gene            4098..4976
                     /locus_tag="VIN75_14400"
     CDS             4098..4976
                     /locus_tag="VIN75_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8975405.1"
                     /translation="MAIFVAVVQEGGFSAAARRLGVSAPVVTRAVSELEAAMNVRLLL
                     RTTRVVKVTEVGERYAQDCRRVLGELEEIEQAASGSHGAVRGRLVVSASVMYGRLKLN
                     EVVLAYLARYPETEVECRFLDRNVNLLGEDVDVAIRIGDLPDASFHATPLTTVRRIVC
                     ASPAYLKRHGTPKQLKDLSKHSMIAALGLEPGSEWKFREGEGVDAPTTAVPVRARLAT
                     DSNDHAIAAACEGFGLTRVLSYMVEEQIASGALVEVLSKFAPPAVPVQALQHQGLRAS
                     RKVRAFIDLAVERLRG"
BASE COUNT          841 a         1739 c         1863 g          815 t
ORIGIN      
        1 aacatgatcc tcatcccggc gctccccgat ttccgtgcgc gtcatcccga gctgcaggtg
       61 gatctgggcg tcagcgaccg gcccatcgat ctgatcagtg aaggcgtgga ttgcgtgatc
      121 cgcggagggg aactggcgga cacgacgttg gtggcgcgtc ggatcgccga cctcgactgg
      181 gtcacctgcg cgagcccggt ttacctgaag gcgcgcggcg tgccgaagca cccgtccgaa
      241 ctgatcgcgc gcgtccacgg atcggaggac cgtgcgcgcg tgccggggca tgcgatcgcg
      301 ggctactttt cctcgctgac cggacgggca tttccactcg agttccaccg cggcgacgag
      361 catctgcaga tcgctggcga gacggtgatg gccgtcaacg aaagcaccgc gcacctgagc
      421 gcgctgctgt cgggcgcggg gctcggacag accttccgct tcgcggcggc accgcatctg
      481 gcctccggcg cgttgcgcac gatccttggc gactggaccc gcccgcgtca tccgctgtat
      541 gtcgtgtacc ccgcttcgcg ccacctgagc gccaagctca ggctgttcgt cgactgggtc
      601 gcggaggtct tcgcgccctt cgacgaccgg ccctagtcag cccgccgccg gcgcgaccgg
      661 ggcggatgcc gctgccggct ttgcccgcaa cgcccacatc accgaggcga tggagagccc
      721 tgcgatcacc aggttgctca tggcgaagag cacgggagcg agcgtggtga agactggatc
      781 gtggccgcgc tcggcaaagc gcatggtgcc ggtggagatc gcggtcgcgc cgaaagtgaa
      841 tgcccagtag gacagcgtga acggctggcg gatccatggc atcagccgca gcaccatcag
      901 cgcctgcacg atcgcgtaac ccagcagcat gcgggcgatg aggtccggcg ggccgacggt
      961 gacggccgca taggccagca gcgcgaccgc cggtggcgcc atctgaatgc ccacggtggg
     1021 acgcagcagg ggcggcaacg gatcgacggt cagcaggcgg ctcaggatga tggactcctg
     1081 gacgatccag gccagcacgc cggcgccgaa gaacagctgc ccccaactcg gccagccgag
     1141 tgccgacatc gcgatcgcgg ataccaggtt ggcgccgacg gtcggcaggt agagcacggg
     1201 cgtcgtcgcc ttctcgtcgc gtccaccacg ccactgcccg ccatggcgcc acagtccgaa
     1261 gagaagcgac gccgccgcac cgacgacgat caggaccgtc cccgggccgt gcgcgtgagg
     1321 tagcacgccg atgccggcga gcatcgtcgc caccggcaca agggcgatga agcagcagag
     1381 gatcggatgg gcggcctcgg cccgggcctc gttgcgcacg gcgatccact tgatcgcgaa
     1441 gcggaccagc agcaccgccc acaccgcgac cgcgaccgcc atgaccgact cgccgatcca
     1501 ggccggcaca ccccagaggc gcgccgccac gcgccagcca ttgcccagac cggtcaggcc
     1561 cagcaccatg ctgaagtagg aggcggggac cgcccatcgg gatgcgttca tcggggtgct
     1621 cctgcgcgag caatgaccgg gaggccatcg gacgagcgca ctgtaaaagg ggagtctggg
     1681 tcgggcaatc ggggcctgtg gggaacgctc gccaaagacc ggtccgcgag ggccgatcgt
     1741 gcgatctgcg ggtctgcgcg ctcgccgttc agagatcgag cgtcagctcg ccgccatccg
     1801 ccctggagac gcagacgcac atcagctgct gctccgactt ctgcgcatcg gtgagcacct
     1861 cgtcgcgatg tgccggtgtt cccgcgacca cccgtgtggc gcaagtgccg cagttgccga
     1921 tgcggcacga ggccggcacc cggacgccct gcgcttcggc gacctcgagg atcgagcggt
     1981 ccttcggtat gacgatgtcc ttgccgctgc gcaccagatg gacgacgaac tcgcgatccc
     2041 ctggcctggt cgtgtccgcg ctgaacttct cggcatgcac gcgcgcggaa gccacgccgg
     2101 ccgacgtcgc ggccgccgac acggcgtcga tcaggcgcga tggcccgcag acatagacat
     2161 ggtcggattc gtccaggccg agcatcaggg cttcgacgtc gaggcggtgg ccttcgtcct
     2221 tcgcatgcac tttcacccgg tcgccgaagg cctcgatcag ctcgcccagg aacggcgcct
     2281 gcgtccgcga ccgcaccgcg tagtgcagct caaactccga gccgcgcgcg atgagcgcgt
     2341 gcgccatcgc cttgatcggc gtgacgccga tgccgccagc gaccagcacc gcccgatgcg
     2401 gcgtctcctc cagcgagaag aggtgcttcg gcatcgaggc gttgagccgc aagccgagcg
     2461 tgaaggcctc atgcacggcc acggagccgc ctcgtccatc ggcctcacgc tgcacggcga
     2521 tctcgtaggc atcggtgcga tgcgggctcg atgcgatcga gtagctgcgc gtcgagtcgg
     2581 tgccgtcggc cagacgcacc ggcaggtcga gatgggcccc ggcgatcact gtcggcagcg
     2641 gcgagccgtc ggcggacctc agctcgaacg cgcgcaccga gggcgtgagc tggcgcacgc
     2701 cggagatcgt cagcgcgagc ggcccgtcgc cgagcgattg cggtgcgctg gcgctgctcg
     2761 gcgccgccga agcggcgagc gccgccttcg cttcgctcaa ctgcgacttc agcttctgca
     2821 cctgcgcgcg cagcggcgcg gtgagcgacg cgacttcggt ctcggtgaaa cgcggcgtga
     2881 tgtgctgcgg gcagttccag tcccagcctt cgatgcggat caggaaggcg cgctcgaccg
     2941 ttgccccata ggacggatcg ctcaccgcgg cgatggcggc gtcgccctgc gcgctgacgc
     3001 cggcctcgac cagctccacg cgcccgagca gcttcagccg gcgccgattc gggtagtcga
     3061 cgacgatcat cgagaggcga tcgtccttgc gcaggttgcc gacgctcagg tactgcacgt
     3121 tgccgcggaa atccgcgaag ccgagggtcc gctggtccag caccttgagg aagcctttcg
     3181 gtccgccgcg gtgctgcacg tagggccagc cggtctcgct gaccgtggcc tggaagaagt
     3241 gatcggcctc ggccaggaac tgcgcctcgc gcggcgtcag cgcatcgccc cggtcgctca
     3301 tcgtgtcgag gaaggcgtac tgctcgcggc tgcccttctc cgactggacg tcgcggaccg
     3361 tcggcgtgaa ggtgatgtcg gaataggcgc gtcccatgat gagctcccgg tgacgatgtg
     3421 gaggttcagg ccgtggccgg cagctcgagc ttgacgctcg ggaagtcgat ctcgaccagg
     3481 ctggccttgc ccaggatgtt ggtcaggaag ttcatgccga cgtgggtgat gatctcgacg
     3541 atctgcgcct cgtcgacacc ggccgcgcgg acctcggtca actccagcgc ggacacctcg
     3601 ccgcgtttct ccatcaggct gcgggcgaac ttcaggatcg cggccgaggt gtcgtcgtcg
     3661 ctggtgccgg cgcgggccgc ggcgatctcc tcggcggaca ggccggtctt gcgtccgatc
     3721 gccgtgtgcg ccgagacgca gtactggcag ccgttctgct gcgccagcgc cagcgcgatg
     3781 cgctcgcggt ccagcggatc gaggctgccg ccgttggcga cgccgtacag gccgaggaag
     3841 gcttgcagcg cggtggtcga gttggcgaag accttcagga agttggggac catgccgagc
     3901 tggccctgga tcgcgtccag cagcgccttc tgctcgggcg tggcggtgga cgattcgacg
     3961 acgtggatgc ggctcatgga agttctccgg tgggttgagg gtgagcggat ggtggccctc
     4021 gcggtccgcg gagagaatcg gcgcggctgg gaaatgactc tttctggaac aggaaagata
     4081 tggacaagtt ggatgccatg gccatcttcg tggccgttgt tcaggagggc gggttttccg
     4141 cggcggcgcg gcggctgggg gtgtccgcgc cggtcgtcac ccgcgcggtg agcgagctcg
     4201 aagccgcgat gaatgtgcgc ctgctgctgc gcaccacgcg cgtggtcaag gtcaccgaag
     4261 tcggagagcg ctacgcgcag gactgccgac gcgtgctcgg cgagctggag gagatcgagc
     4321 aggcggccag cggctcgcac ggcgcggtgc gaggcaggct cgtcgtcagc gcctcggtga
     4381 tgtacgggcg gctcaaactc aacgaggtgg tgctggccta tctggcgcgg tatccggaga
     4441 ccgaggttga atgccggttc ctcgatcgca acgtcaacct gctcggggag gacgtcgacg
     4501 tcgccatccg catcggtgac ctgccggacg cttccttcca tgcgacgccg ctgaccaccg
     4561 tgcggcggat cgtgtgcgcg tcaccggctt acctcaagcg gcacgggaca ccgaagcagc
     4621 tgaaggatct ttcgaagcac tcgatgatcg cggcgctcgg cctggagccg ggatccgaat
     4681 ggaagttccg cgagggggag ggcgtcgacg ctccgaccac ggccgtgccg gtccgggctc
     4741 ggctcgccac cgacagcaac gaccacgcca tcgccgccgc ttgcgagggg tttggcctca
     4801 cccgcgtgct gtcctacatg gtcgaagagc agatcgcttc gggcgcgctg gtggaggtgc
     4861 tctcgaagtt cgcgccgccc gcggtgccgg tgcaggcgct gcagcaccag gggctgcgag
     4921 cgtcgcgcaa ggtgagggcg ttcattgacc ttgcggtgga gcggttgagg gggtgaggga
     4981 gcaatcgccg cgaccggtca aggcgatcac ggcggcgttg cctggccgct gttgggccga
     5041 tcgacctttt ggggccgcgt tccaggtcag cctagcggct cgcctttgcg aaccaggatg
     5101 cacagaaagc ccgtcgtgaa tagggctgag aggccgaaga agacaagggg atgcgcgctt
     5161 ggtgagcgaa acgctgccag acctccgaga gacgagagat aggcagccag cgtcacccat
     5221 ccttgccacc tttgaggcag accccagccg tatccaaa
//
LOCUS       DATCRO010000526         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_804414, whole genome shotgun sequence.
ACCESSION   DATCRO010000526 DATCRO010000000
VERSION     DATCRO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804414"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..647
                     /gene="rsmD"
                     /locus_tag="VIN75_14405"
     CDS             57..647
                     /gene="rsmD"
                     /locus_tag="VIN75_14405"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY8975406.1"
                     /translation="MTPAQKRERRAAEHAPHEIRIIGGQWRRRLLPVPDMPGLRPTPD
                     RVRETLFNWLGQDLDGWSVLDAFAGTGALGFEAASRGAATVQMLERETKLVSGLAATR
                     TKLDAAVVTITQADALAWMKRAPAQFDLVLLDPPFDANLFEPALAASLNCVVPQGWIY
                     LEAPAPLDAAALAERGLELYRHVRAGNVHAHLLRRT"
     gene            764..1261
                     /gene="coaD"
                     /locus_tag="VIN75_14410"
     CDS             764..1261
                     /gene="coaD"
                     /locus_tag="VIN75_14410"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8975407.1"
                     /translation="MTKLTAVYPGTFDPMTLGHQDLMRRASALFPRLILAVAAGHHKR
                     TMFSVEERLEIAQELAQPYKNVEVVAFKGLLRDFVVANGGKVVVRGLRAVSDFEYEFQ
                     MAGMNRQLMPDVETLFLTPSDQYQFISGTFVREIAALGGDVSKFVAPLVLSKLKDKIS
                     RPREE"
     gene            1268..1573
                     /locus_tag="VIN75_14415"
     CDS             1268..1573
                     /locus_tag="VIN75_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY8975408.1"
                     /translation="MALMITDECINCDVCEPECPNEAISMGEEYYVIAPDKCTECVGH
                     FDSPQCVQVCPVECIPINPEHVETKEQLMTKYRRLLGLDGAVPAAALASSPHAPSAG"
     gene            complement(1676..2293)
                     /locus_tag="VIN75_14420"
     CDS             complement(1676..2293)
                     /locus_tag="VIN75_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975409.1"
                     /translation="MTNQTTRFHLPSLIASRARALARDARPHGRAPTHARRLALARTP
                     AIATALWGALVLGVDARQARASHAHKAASAPSTEQILAPSAATLPDALPPERQVWRCG
                     NSYSRHPCADAAAKPLDVADPRSDAQRRQSEDVTARDKRLAAWYEAARRQRETPASAP
                     APARAPAASAACVDTTMMHCVPKKPRTRKATLPGRAGAPSAGHGP"
     gene            complement(2290..3006)
                     /gene="pth"
                     /locus_tag="VIN75_14425"
     CDS             complement(2290..3006)
                     /gene="pth"
                     /locus_tag="VIN75_14425"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8975410.1"
                     /translation="MIRLIVGLGNPGAEYEATRHNAGFWWVDQAVRQLKGSLVHDPKY
                     HGFVARVNRPEGPVWLQQPLTYMNLSGKAVAPLARFFKILPEEILVVHDELDIPPGQM
                     KLKKGGGNGGHNGLKDIQAQLGTGEFWRLRLGIGHPGDRNEVAAYVLRKPPAAERELI
                     EKNVDESLKALDLLLAGDMDKASMKIHAKPPRPKPPRPPQLNPLLTSLMNKVPAATPS
                     PTSPASPAGPASAGTQEPAE"
     gene            complement(3097..4038)
                     /locus_tag="VIN75_14430"
     CDS             complement(3097..4038)
                     /locus_tag="VIN75_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975411.1"
                     /translation="MLILETDRLRLRWFNDTAADADFLRALLNDPGWLANIGERNVRT
                     RRQARHWIATRHTAVYGRLGFGFWAVERKSDGALMGMCGLIKRDTLLEVDVGYALMPA
                     FRGQGYAREAASACVRYAHDVLGMPEVWGITNPDNAASAIVLQQIGLRDAGITRLVGN
                     DYDTWMFKSPRVEPGDDQRQIDALTRRFLAAFTNRGGVIPTLPALPHYFMLDATVRVA
                     DSLGTVTTTGVHGFIAPRAELLARGRLVDFEEHETESRTEIRGAIAQRWLRYAKRGTL
                     DGQPFEGAGTKALQFVRTARDWKIASLMWTDDITPGA"
     gene            complement(4060..5007)
                     /locus_tag="VIN75_14435"
     CDS             complement(4060..5007)
                     /locus_tag="VIN75_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEY8975412.1"
                     /translation="MNLLDPAALESLAALAEAGSFERAAQLLSITQSAVSQRLRALET
                     QVGQLLVVRSRPLRMTEPGKVLLRYARQLQALRADAARELGAAQMREERLPIAINADS
                     LATWVLGALDALVHAGQREGYGLELIVDDQDFTHDRLREGEVLGCVSTVSMALRGCTC
                     QPLGHMRYIAVASPAFVELAMPGGLNEANFARLPFIVFNRKDDMQAQWVARAMGVRDP
                     RLKERFVPSSEAGARAAVMGWGVAVIPELLARAHLDAGALVAIRPEVTIDVALYWHQW
                     KLGTGIDAADGNAPIARSGLMEQIGQALIAGARAALLPP"
     gene            5110..5391
                     /locus_tag="VIN75_14440"
                     /pseudo
     CDS             5110..5391
                     /locus_tag="VIN75_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365921.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
BASE COUNT          773 a         1895 c         1934 g          789 t
ORIGIN      
        1 cgcgcacgcc gcgcgccgcg cgtggcgccg cggccgtgcc cccggcaccc gcgccgctga
       61 cgcccgccca gaagcgcgag cgccgcgccg ccgagcatgc gccgcacgag atccgcatca
      121 tcggcggcca gtggcgccgc cgcctgctgc cggtgcccga catgccgggc ctgcgtccca
      181 cgcccgaccg cgtgcgcgag acgctgttca actggctggg ccaggacctg gacggctgga
      241 gcgttctcga cgccttcgcc ggcaccggcg ccctcggctt cgaggccgcg tcccgcggcg
      301 ctgccacggt gcagatgctg gagcgcgaga ccaagctggt ctccggcctg gccgccacca
      361 ggacaaagct cgacgcggcc gtcgtcacga tcacgcaggc cgacgcgctg gcctggatga
      421 aacgcgcccc cgcgcagttc gacctggtgc tgctggaccc gccgttcgac gccaacctgt
      481 tcgagccggc gctggccgcg tcgctgaact gcgtcgtgcc gcagggctgg atctacctgg
      541 aggcgccagc gccgctggat gccgcggcgc tggccgagcg cggcctggag ctgtatcgcc
      601 acgtgcgggc cggaaacgtc cacgcgcacc tgctgcgcag gacgtgaccc atcgcgcgcc
      661 tacgcaacat cgcctaatcc gcgtgcacct cggctgtgga atgatcgcgc cgtagccaac
      721 gaacgagaca acgcggcctt gtccgcgccc cgcaaggccc tccatgacca agctgacagc
      781 cgtctacccc ggaaccttcg acccgatgac cctcgggcac caggacctca tgcgccgggc
      841 cagtgcgctg ttcccgcgcc tgatcctggc cgtggccgcc ggccatcaca agcgcaccat
      901 gttctcggtg gaggagcgcc tggagatcgc ccaggaactg gcccagccct acaagaacgt
      961 cgaggtggtg gccttcaagg gcctgctgcg cgacttcgtg gtggccaacg gcggcaaggt
     1021 cgtggtgcgc ggcctgcgcg ccgtcagcga cttcgagtac gaattccaga tggccggcat
     1081 gaaccgccag ctgatgcccg acgtcgagac gctgttcctg acgccttccg accagtacca
     1141 gttcatcagc ggcaccttcg tgcgcgagat tgccgctctc ggcggtgatg tctccaaatt
     1201 cgtggcaccc ttggtgctgt cgaagctgaa ggacaagatt tcccggcccc gggaagagtg
     1261 agcggtcatg gccctgatga tcacggacga gtgcatcaac tgcgacgtct gcgaacccga
     1321 atgccccaac gaggccatct cgatgggtga ggagtactac gtcatcgcgc cggacaagtg
     1381 caccgagtgc gtcggccact tcgactcccc gcagtgcgtg caggtctgcc cggtcgagtg
     1441 catcccgatc aatccggagc acgtggagac caaggagcag ctgatgacca agtaccggcg
     1501 cttgctcggg ttggacggcg ccgtgccggc ggcggccttg gcctcgtcgc cgcacgcacc
     1561 gtccgcgggt tgacgcggcc aggcgcgcgg accgcgggtc tcgacccgcc ctgcccaccg
     1621 ttcatgggcg ccggcatcct tgcgacacca ccgctggcgg gtcgaacgat ccgccctacg
     1681 ggccgtgacc cgccgacggg gcgcccgcgc ggcccggcaa ggtcgccttg cgcgtgcgtg
     1741 gcttcttcgg cacgcaatgc atcatcgtcg tgtcgacgca cgcggccgag gccgcagggg
     1801 cgcgcgccgg ggccggcgcc gaggccggcg tctcgcgttg ccgccgcgcc gcctcgtacc
     1861 aggcggccag gcgcttgtcg cgcgcagtga cgtcctccga ctgccggcgc tgcgcgtccg
     1921 agcgcggatc ggcgacgtcc agcggcttgg ccgccgcatc cgcacacgga tggcgcgaat
     1981 agctgttgcc gcagcgccag acctgccgtt ccggcggcag cgcatcgggc aaggtggcgg
     2041 ccgagggcgc caggatctgc tcggtggacg gcgccgacgc cgccttgtgt gcatgagatg
     2101 cgcgcgcctg gcgggcatcg acacccagca cgagcgcacc ccacagcgcg gtggcgatgg
     2161 cgggcgtgcg cgcgagcgcg agtcggcgtg cgtgtgtggg cgcgcggccg tgcggacgcg
     2221 cgtcacgcgc tagggcgcgc gcacgcgagg cgatcaggga aggaaggtgg aaacgggtcg
     2281 tctggttcgt cactcggcgg gctcctgcgt gccggccgaa gccggccccg cagggctcgc
     2341 aggggacgtc ggcgacggcg tggccgccgg caccttgttc atcagcgacg tcagcagcgg
     2401 attgagctgc ggcggacgcg gaggcttggg gcgcggcggc ttggcgtgga tcttcatgct
     2461 cgccttgtcc atgtcgcccg ccaggagcag atcgagagct ttcaggctct cgtcgacatt
     2521 cttctcgata agttcgcgtt cggcggccgg cggcttgcgc agcacgtagg ccgcgacctc
     2581 gttgcgatcg cccggatggc cgatgcccag ccgcaggcgc cagaactcgc ccgtccccag
     2641 ctgcgcctgg atgtccttca ggccgttgtg gccgccgttg ccgccgccct tcttgagctt
     2701 catctgcccc ggcgggatgt cgagctcgtc gtgcacgacg aggatctcct cgggcaggat
     2761 cttgaagaag cgcgcgagcg gcgccaccgc cttgcccgac aggttcatgt aggtcagcgg
     2821 ctgctgcagc cacaccgggc cctcgggccg gttcacgcgc gcgacgaagc cgtggtactt
     2881 cgggtcgtgc acgagagagc ccttgagctg gcgcaccgcc tggtccaccc accagaagcc
     2941 cgcgttgtgg cgcgtggcct cgtattccgc cccggggttg ccgaggccga cgatcagtcg
     3001 aatcatgggg cgattatccg cgggcgcggc ccggggccgg cgacatggcc tggggtggat
     3061 cctgcgactt cgcgcaggat gacgaacggg ggcggttcag gcgcccggcg tgatgtcgtc
     3121 cgtccacatc agcgaggcga tcttccagtc gcgcgcggtg cgcacgaact gcagggcctt
     3181 ggtgccggcg ccctcgaagg gctggccgtc gagcgtgccg cgcttggcgt agcgcagcca
     3241 gcgctgcgcg atggcgccgc ggatctcggt gcgcgattcg gtctcgtgct cctcgaagtc
     3301 gacgaggcgg ccgcgcgcga gcagctcggc gcgcggcgcg atgaagccgt gcacgcccgt
     3361 ggtcgtgacg gtgccgagcg agtcggccac gcgcacggtg gcgtccagca tgaagtagtg
     3421 cggcagcgcg ggcagggtcg ggatgacgcc gccgcggttg gtgaacgcgg ccaggaagcg
     3481 gcgggtcagc gcgtcgatct ggcgctggtc gtcgcccggc tcgacgcgcg gcgacttgaa
     3541 catccacgtg tcgtagtcgt tgccgacgag ccgcgtgatg ccggcgtcgc gcagcccgat
     3601 ctgttgcagc acgatggcgg acgcggcgtt gtccgggttc gtgatgcccc agacctcggg
     3661 catgcccagc acgtcgtggg cgtagcgcac gcaggccgat gcggcctcgc gggcatagcc
     3721 ctggccgcgg aacgcgggca tcagcgcgta gccgacgtcg acctccagca acgtgtcgcg
     3781 cttgatcagc ccgcacatgc ccatcagcgc gccgtcggac ttgcgctcga ccgcccagaa
     3841 gccgaagccg aggcgcccgt agacggcggt gtggcgcgtg gcgatccagt ggcgcgcctg
     3901 gcgccgcgtg cgcacgttgc gctcgccgat gttggcgagc cagccggggt cgttgagcag
     3961 ggcgcgcagg aagtcggcgt cggcggcggt gtcgttgaac cagcgcaggc gcaggcggtc
     4021 ggtctcgagg atcagcatgg gtgcgtcagg tcgttgtgtt cagggcggca gcagcgccgc
     4081 gcgcgcgccg gcgatcagcg cctggccgat ctgctccatc aggcccgaac gcgcgatcgg
     4141 tgcattgccg tcggccgcgt cgatgccggt gcccagcttc cactggtgcc agtacagcgc
     4201 gacgtcgatc gtgacctcgg ggcggatcgc caccagcgcg cccgcatcga ggtgcgcgcg
     4261 ggcgagcagc tcggggatca ccgcgacgcc ccagcccatc accgcggcgc gcgcgccggc
     4321 ctcgctggac gggacgaagc gctccttcag gcgcggatcg cgcacgccca tcgcgcgcgc
     4381 cacccactgg gcctgcatgt cgtccttgcg gttgaacacg atgaagggca ggcgcgcgaa
     4441 gttggcctcg ttcaggccgc cgggcatcgc gagctcgacg aacgcagggc tcgcgacggc
     4501 gatgtagcgc atgtggccca gcggctggca ggtgcagccg cgcagcgcca tggacaccgt
     4561 cgacacgcag cccagcacct cgccctcgcg cagccggtcg tgcgtgaagt cctggtcgtc
     4621 gacgatcagc tccagaccgt agccttcgcg ctggcctgcg tgcaccagcg cgtcgagcgc
     4681 gcccagcacc caggtggcca ggctgtcggc gttgatggcg atgggcaggc gctcctcgcg
     4741 catctgcgcg gcgccgagct cgcgcgcggc gtcggcgcgc agcgcctgca gctggcgtgc
     4801 gtagcgcagc agcaccttgc cgggctcggt catgcgcagc gggcgcgagc gcaccaccag
     4861 cagctggccc acctgcgtct cgagcgcgcg cagccgttgc gagacggccg actgcgtgat
     4921 cgacagcagc tgcgccgcgc gctcgaagct gccggcctcc gcgagggcgg ccagcgattc
     4981 cagtgcggcg ggatccagca ggttcataag caacgctact gtaatcgctg caatttcgat
     5041 gaatcttcac actttccctg ggccggccta cgctggatcg accacgaatt catccaaccg
     5101 gagacaagca tgcagatcgg cgttccgaag gaaatcaaga accacgagta ccgagtcggc
     5161 ctgacgccgg ccagcgtgcg cgagttcgtc gcgcacggcc acgccgtgat ggtgcaggcc
     5221 ggcgcgggcg cggccatcgg cctggacgac gcccagtacc gcgcggcggg tgcgacgatc
     5281 gtcgacagtg cgcaggaggt gttcgcaagg ccgacatggt cgtcaaggtc aaggagccgc
     5341 agccgcagga gtgcgcgatg ctgcgtcccg gccagctgct ctacacctac c
//
LOCUS       DATCRO010000527         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_669916, whole genome shotgun sequence.
ACCESSION   DATCRO010000527 DATCRO010000000
VERSION     DATCRO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669916"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VIN75_14445"
     CDS             <1..1828
                     /locus_tag="VIN75_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY8975413.1"
                     /translation="YYRGPGWYRHELLLPPAPVAARRFLQFDGAALVTDVWVNGHPAG
                     HHEGGYGAFRFDVGRWLHAGRNAVAVRVDNAPSNRVAPLGGDFTVFGGLYRTVELVTV
                     RPVHFDLLDDGGPGVYVAAGRVAPDRAELAARARLCVPDGARGSMHVRWTLLDARGRR
                     VVRSSTRVAAGPAGIRTIRQALSVPHPRLWQGVEDPALYRLRVEVLYRGAVADVVDVP
                     VGIREFSIDPVRGLVLNGQPYAVHGVDLFHSGRPGHGLAVTDAQVDEDFEALGELGVT
                     GLRLVHFQHPQRAYDDADKSGFVVWTEIPLNSSMDESSAFRANLSQQLRELVKQNYNH
                     PSVLIWGLGNEVYKSDAASHALLADLQREAKALDPHRLTSYAHCCAADDDPLTRQTDV
                     IAYNRYYGWYDGQVADIGPWADRLHAARPGRAIAVSEYGAGASVLQQEEPPARPVPAS
                     HWHPEQYQALFHEAYWRQLSARPWLWGTFVWVAFDLPSAGRDEGDRRGVNDKGLATYD
                     RRIRKDAWYWYQANWSSRPMLHLADRRAAPRAGAGSNVKVYTNLDEVTLAVDGASLGS
                     RRASNRVATWEDVKLPPGVHHLTATGGGVNDSIEWIVAAPEQ"
     gene            complement(1856..2563)
                     /locus_tag="VIN75_14450"
     CDS             complement(1856..2563)
                     /locus_tag="VIN75_14450"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEY8975414.1"
                     /translation="MAEAINKAGRQRMLSQRLAKSYMARGQDVDSGLADRTLDASMSM
                     FERQLGEPRSLAFQGGVADALRRLEASWRPYRAALTAGPPDRTRAPAVLSCAADVLAR
                     AQECTALLVAMSGQAIGRCVDTSGRQRMLSQRIALLYLSSSWGLEGASAQAEIASGSA
                     KFAAAQAALLSAPESTAAIRDQLALANQQWTFFGAALRGLRPGVADPEAMSHVFTTSE
                     RILEVMEDVTGQYAKSG"
BASE COUNT          465 a         1093 c         1138 g          482 t
ORIGIN      
        1 ctactaccgc ggccccggct ggtaccgcca cgagttgctg ctgccgccag ctcccgtcgc
       61 ggcaaggcgc ttcctgcagt tcgacggcgc ggcgctggtc acggacgtct gggtcaacgg
      121 ccatccggcg ggacaccacg aaggcggcta cggcgcattc cgcttcgacg tcggccggtg
      181 gctgcatgcg ggccgcaacg ccgtcgccgt gcgtgtcgac aatgcaccct ccaaccgcgt
      241 ggccccgctg ggcggggact tcacggtgtt cggcgggctg taccggaccg tcgagctcgt
      301 gaccgtgcgc cctgtccatt tcgatctgct ggacgatggc gggccgggag tctatgtcgc
      361 tgcaggcagg gtcgcgccgg atcgcgcgga gcttgcggcc cgggcgcggc tgtgcgttcc
      421 cgacggggcc cgcgggagca tgcatgttcg ctggacgctg ctggatgcgc gcgggcgccg
      481 ggtcgttcgc tcgtcgacac gtgtcgccgc agggcctgcc ggcattcgga cgatcaggca
      541 ggccttgtcg gtgccgcacc ctcgactgtg gcaaggcgtg gaggacccgg cgctctacag
      601 gttgcgagtg gaagtgctgt accgcggcgc cgtcgccgac gtggtggacg tgcccgtcgg
      661 aatccgcgag ttctccatcg atccggtccg gggcctcgtc ctgaacggcc agccctacgc
      721 cgtgcacggc gtcgacctgt tccacagtgg ccgccccggc cacggcctgg ccgtcaccga
      781 cgcccaggtg gacgaggatt tcgaggcgct cggcgaactc ggcgtgacgg gattgaggct
      841 cgtccacttc cagcatcccc aacgcgccta tgacgacgcc gacaagtcgg gattcgtcgt
      901 ctggaccgag attccgctga actcgtccat ggacgagagc agcgcgttcc gggccaatct
      961 cagccagcaa ctgcgcgaac tggtcaagca gaactacaac catccgtcgg tgctgatctg
     1021 gggcctgggc aacgaggtct acaagtccga cgcggcaagc catgcgcttc tcgcggactt
     1081 gcagcgcgag gccaaggcgc tcgatccgca tcgactcacg agctacgcgc attgctgcgc
     1141 cgccgacgac gacccgctga cgcggcagac cgacgtgatc gcatacaacc gctactacgg
     1201 ttggtacgac ggccaggtgg ccgacatcgg gccctgggcc gaccgcttgc acgcggcccg
     1261 gcccgggcga gccatcgccg tgagcgaata tggcgccgga gccagcgtgc ttcaacagga
     1321 ggagccgccg gcgcgtccgg taccggcgag ccactggcat cccgagcagt accaggcgct
     1381 gttccacgag gcctattggc gccagctcag cgcgcgcccg tggttgtggg gcacgttcgt
     1441 gtgggtcgca ttcgacctgc cttccgccgg ccgcgatgaa ggcgatcgac ggggcgtcaa
     1501 cgacaagggc ctggcgacgt acgacaggcg gatccgcaag gatgcgtggt actggtacca
     1561 ggccaactgg agctccaggc ccatgctgca ccttgccgat cgccgcgccg cgccgcgtgc
     1621 cggggccggc tcgaacgtca aggtctacac gaacctggac gaggtgacgc ttgctgtcga
     1681 cggtgcgagc ctgggatcgc gccgtgcatc gaaccgcgtc gcgacctggg aagacgtaaa
     1741 gctgccgccc ggggtccacc acctcaccgc caccggcggc ggggtgaacg actcgatcga
     1801 atggatcgtg gcggcgccgg agcaatgacc cggcggccgg cgggcggcct tggcctcagc
     1861 ccgatttcgc gtactggccc gtcacatcct ccatcacctc caggatgcgc tcgctggtcg
     1921 tgaacacgtg gctcatcgcc tccgggtccg ccacgcccgg gcgcaagccc cgcagcgcgg
     1981 cgccgaagaa cgtccactgc tgattggcca gcgcgagctg gtcccggatc gcggccgtgc
     2041 tctcgggcgc cgacaggagg gcggcctgtg cagccgcgaa cttcgcgctg ccgctcgcga
     2101 tctcggcctg cgcggaggcg ccttccagtc cccagctcga gctgagatag agcagggcga
     2161 tgcgttggct cagcatccgc tggcgtccgc tggtatcgac gcagcgcccg atcgcctggc
     2221 cggacattgc caccagcaac gccgtgcatt cctgggcccg tgcgagcacg tcggcagcgc
     2281 acgagagcac cgcaggcgcg cgagtgcggt ccggcggccc ggccgtcaat gcggcgcggt
     2341 agggccgcca actggcctcc aggcggcgca aggcgtcggc gactccgccc tggaatgcga
     2401 gtgacctggg ctcgcccagt tgccgctcga acatcgacat cgaggcgtcg agcgtcctgt
     2461 ccgccagccc gctgtcgacg tcctgcccgc gggccatgta gctcttggcc aggcgctggc
     2521 tcagcatgcg ctgccggccc gccttgttga tggcctcggc catctcgtgc gtgtcggcca
     2581 ggaccgtgcc gctcgccagg gtcgacgcga tgaccgcgag gcagtcgcgc cgcctcatgc
     2641 cgcgctcccg ccgagacggg gacggtcggg acgccaggca ggcaggatgt ggtggcggcg
     2701 ggagcgccgg agacatgcat ctgtgaacat gcggtgcttt cgaaaggcaa gcgacggatg
     2761 aatgccgcct acgtgcagtg gcggcacggc gtgaacgtga atgggggtga cgtcgcgcgc
     2821 ggcgcggacg acagggacga gttggcaggg cgatcgcgcg gacggccgga ttgcgccggg
     2881 cgacgatcac gggcgcgctt gcgcggcggc agggcccgat cgcctcaggg gtgcttcgtt
     2941 gcaccacgcc aggcaaagca tgaagcgtgc ctctgctgaa gcagcggttc gtcgcgtgcg
     3001 cgagcgcgcc cggccgggag acgacggttc tcaggacctt ttcttggcct cgcacgcgcc
     3061 atgtcggtgc cgaccatgcg ccatggaggg ccgtggctag accggttgcc gccgggtggt
     3121 gcggccggtc acggcgggcg ctgcacgcgc gccgtcaggc gacgaggccg tccagttc
//
LOCUS       DATCRO010000528         9337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_849940, whole genome shotgun sequence.
ACCESSION   DATCRO010000528 DATCRO010000000
VERSION     DATCRO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849940"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VIN75_14455"
     CDS             <1..258
                     /locus_tag="VIN75_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEY8975415.1"
                     /translation="VEKAKSTDTDKVIAAMAGQTFKAPSGFVLEMDPKNHHLHKPVFI
                     GEVKADGQFNVVWKTPAPIKAQPWSPYIPEDKGKKDEPEKK"
     gene            277..1881
                     /gene="urtB"
                     /locus_tag="VIN75_14460"
     CDS             277..1881
                     /gene="urtB"
                     /locus_tag="VIN75_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY8975416.1"
                     /translation="MRKWLSRGVRIVLAAALGTAAAMPACALDAAQARAIAVGDGDAR
                     IAALDAAVERADPGLAAFAQALLDDAVKTGANGVYVVRDDKVTDAVTGAPAALPDDAE
                     DVTNNNRMRGALQAALSGLRLLSRDAAQRHAAIDELSRMDPDPALLPVLDKAWAAEAD
                     GHAKRQLDRLRAAVRIAAPDRAQRQQAIDALSRSDEPAVRALLQARLAPDAERDPELR
                     AALARALAQVDGRLAWGERLGMLFSGLSLGSILLLVALGLAITYGLMGVINMAHGELM
                     MIGAYATWLVQNLFRHYAPHWFDAYLILAIPVSFLVAALVGAVLERGVIRFLYGRPLE
                     TLLATWGISLVLMQLVRTLFGAQNVEVENPSWMSGGIAVASNLVLPWNRLAIIVFAFL
                     VLAATGLLISRTRLGLFVRGVTQNRRMAACTGIDTARTDTMAFALGAGIAGLAGCALS
                     QVGNVGPDLGQGYIVDAFMVVVLGGVGQLTGTVLAALGLGMMNKALEGIAGAVLAKIL
                     VLVFIVVFIQKRPQGLFALKGRSAES"
     gene            1878..3026
                     /gene="urtC"
                     /locus_tag="VIN75_14465"
     CDS             1878..3026
                     /gene="urtC"
                     /locus_tag="VIN75_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY8975417.1"
                     /translation="MSPEVKPVRPVHLLPEARRGLLLGARGWTAVAGTLLLVAAVVPV
                     LNLLVPSGHMLHLGDWGVALIAKIMCYAICALAMDLIWGYTGILSLGHGLYFALGGYA
                     MGMYLMRQIGHDGNYRSDLPDFMVFLDWKELPWTWAGSASLAFQLVLVVAVPALVAGV
                     FGWFAFRSRIKGVYFSIITQALTFAAMLLFFRNETGFGGNNGFTDFKRIAGVPIALPS
                     TRMALFVVTGCTLLGFWLLARAIVNSKFGRVLQAIRDAESRVMFTGYDPLRYKLAIWV
                     LSAAMCGVAGALYVSQVGIINPSEMSPSASIEMAVWCAVGGRGTLVGPILGAFFVNGA
                     KSWFTQAFPEFWLYFLGALFIAVTLFLPRGLMGIAASLRREKGMEETR"
     gene            3023..3895
                     /gene="urtD"
                     /locus_tag="VIN75_14470"
     CDS             3023..3895
                     /gene="urtD"
                     /locus_tag="VIN75_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY8975418.1"
                     /translation="MTPELMEQGIAVLERAHAREAAAPSGGASFGRVLQPRVPDFAHG
                     VALYLDAISVSFDGFKALNELSLAIDVGELRCIIGPNGAGKTTMMDVVTGKTRPDAGH
                     AHFGSNIDLLRLREPEIVRAGICRKFQKPTVYEQLSVFENLELALAADRGVRAALTAR
                     VTPLQRERIAEILEVVHLLPEARRIAGLLSHGQKQWLEIGMLLAQEPVLLLLDEPVAG
                     MTDEETERTAELFLALAGKHSLVVVEHDMKFVGMLTQGHRKVTVLHEGAVLAEGTLAD
                     VQANPQVIDVYLGR"
     gene            3896..4588
                     /gene="urtE"
                     /locus_tag="VIN75_14475"
     CDS             3896..4588
                     /gene="urtE"
                     /locus_tag="VIN75_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEY8975419.1"
                     /translation="MLEVESLNQYYGGSHILRDVSFEARAGEVTVILGRNGVGKTTLL
                     KSLMGVVAVRAGSIRLGGHELAGLAPYERVRRGAGYVPQGREIFGRLTVMDNLRMGLA
                     SRPAREDVPAELFDLFPVLAQMRDRRGGDLSGGQQQQLAIARALAPGPDLLLLDEPTE
                     GIQPSVIKDIGRVIRRLADRKTMAIVLVEQYYDFAAELADRYIVMERGEIVARGDGST
                     MAADGVRALMSV"
     gene            complement(4633..5493)
                     /locus_tag="VIN75_14480"
     CDS             complement(4633..5493)
                     /locus_tag="VIN75_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY8975420.1"
                     /translation="MAWHARLDLDYRTDSNGSAGSTRTCVDFRHDGPLRILKSLHPEG
                     EAVCHSVIVHPPGGIVGGDTLDLSIGLAPRTHALLTTPGATRFYRSSGARAAQVVDAR
                     VADGARLEWLPLETLVHSGALADNRLRFDLAPGAEMIGWDCVALGLPAAEAPFLRGDY
                     RHDIALSAAGRPRWLERGRTAATDLALLDGPCGWAGHRALGTLWFAAGAQMSDERREA
                     LLHIARDVAAAHSLAATVGATAPQAEVVVVRMLAPRIEPAFELMTQIWKRWRTFAWTL
                     APCPPRVWRT"
     gene            complement(5556..7613)
                     /locus_tag="VIN75_14485"
     CDS             complement(5556..7613)
                     /locus_tag="VIN75_14485"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY8975421.1"
                     /translation="MFITKPMKKTLAACLLALATLQTVLAQGADGHPDWPGAGQLFVG
                     TCYQPVDRSLEQVKRDVALMKQAGFKVVRMGDLSWDYFEPQEGRFDFRHFDAVMNEMQ
                     AAGIKVILDIPGLPAPTWLHHKYPGVDIVNQAGTRLAPAERYMDDIGDPDYRRLAIRM
                     ADTLTRRYARHPALLAVGYDNEIGNGFMSYSEADRKRFIAWLKKRYGSLEALNKAWAT
                     QRWSRRIGQWDEVRLPYGDGPGPAERYLDLRRFWSDITIATLTDLEAVRKKNTPDTPT
                     LSNYWDWADRKGFDYLGSYRRHTTYGAMGFYAGDALDGGWQAALIKGGMSTPTWFNEF
                     AAGGGGSYGSRGWSRMWAHFVLLMGGQAAVAWTFNSHLGGEEQALMGLIDHDDRPSWK
                     LAEWARIASDYARLEKLGFPRKVEPKVAIAYSFENTAAYSAPGPSNTSRQYLKTPYLV
                     QAHGAFEPLLKDNVDVAVIKLSYEDLSRYRLIVVPGMYLLDEASTDALRKFVADGGTV
                     VMTAQSAKVDGHDQWHATPLPGGLTDVFGLRTNEFYDAAGTLEIGGEQVEGTIEHYEV
                     LEPSTAQVMSRFSNLEGMPPAITVNRFGKGRAIYVATPAQPQIMRPLYRQLYAQLGIE
                     PGPRTPDGVFARAVDGRVLYVNTNNAPVDVAIDGAMKGVLGGQRWDGSLRLEARGVEL
                     LER"
     gene            complement(7674..>9337)
                     /locus_tag="VIN75_14490"
     CDS             complement(7674..>9337)
                     /locus_tag="VIN75_14490"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY8975422.1"
                     /translation="YAVRVRVVTDGQVVVEGDRLSIRGARHALLLVAGETSYRRFDDV
                     SGDPVAAVRDRTEVAASKGLDTLMAEHLASHRALYRRMTLRLGKPSAAAPPTDRRIRP
                     PSDPLEPDFAVLYLNYARYLLISSSRPGGQPANLQGLWNEGNNPPWGSKYTININTEM
                     NYWLAGPANLHECVEPLLRMVEDLAVTGARTARLQYGARGWVAHHNTDLWRATAPIDG
                     PMWGLWPMGGAWLCLSLWDEFHYGQDDALLRRLYPLMRGAAEFFLDTLVEDPEGRGLV
                     TSPSISPENAHGAGVAICAGPAMDRQILRDLFAAVLDAQRRCGVADDAFAESVSRARA
                     RLPADRIGAQGQLQEWLDDWDAQAPDQRHRHVSHLYGLFPSDQINVRDTPALVRAAKT
                     TLNTRGDLSTGWATAWRLALWARLGDGQRALGILQGLLSTERTYPNLFDAHPPFQIDG
                     NFGGAAGMLEMLVQSWGGEIRLLPALPAAWPEGELRGVRARGGVEIDLAWSKGRLTRA
                     ALRGRPGAVLRVRHAGGLMTLALDAEGKAQWPPRRPARTHSAKRT"
BASE COUNT         1358 a         3176 c         3301 g         1502 t
ORIGIN      
        1 gtggagaagg ccaagagcac cgacaccgac aaggtgatcg cggcgatggc cggccagacc
       61 ttcaaggccc cgagcggctt cgtgctggag atggatccga agaaccacca cctgcacaag
      121 ccggtcttca tcggcgaggt caaggcggac ggccagttca acgtggtgtg gaagacgccc
      181 gcgcccatca aggcgcagcc ctggagcccg tacatcccgg aggacaaggg caagaaggac
      241 gagcccgaga agaagtgagc tgacggagcg gcgacgatgc gcaagtggct ttcccgggga
      301 gtgcggatcg tgctggccgc ggcgttgggc accgcggccg cgatgcccgc ctgcgcgctc
      361 gacgccgcgc aggcgcgcgc catcgcggtc ggcgatggcg acgcccgcat cgcggcgctc
      421 gacgccgcgg tcgaacgagc cgacccgggg ctcgccgctt tcgcgcaggc gctgctggac
      481 gacgccgtga agaccggcgc caacggcgtc tatgtcgttc gcgacgacaa ggtcaccgac
      541 gccgtcaccg gcgcgccggc ggccttgccg gacgacgccg aagacgtcac caacaacaac
      601 cgcatgcgcg gcgcgctcca ggcggcgctg agcggcctgc gcctgctgtc cagggacgcg
      661 gcgcaacgcc atgccgcgat cgacgagctg tccaggatgg atccggatcc cgcgctgctg
      721 ccggtgctgg acaaggcctg ggcggcggaa gccgatggac atgcgaagcg gcagctcgac
      781 cggctccggg ccgccgtgcg gatcgccgcg cccgaccgcg cgcagcggca gcaggcgatc
      841 gatgccctct cgcgcagcga cgaacctgcc gtgcgcgcgc tgttgcaggc gcgcctcgcg
      901 cccgacgccg agcgcgatcc cgaactgcgc gccgcgctcg cgcgagcgct cgcccaggtc
      961 gacggccggc tcgcgtgggg cgaacggctt ggcatgttgt tctcgggcct gagcctgggc
     1021 tcgatcctgc tgctcgtcgc gctcggcctg gcgatcacgt acggcctgat gggcgtgatc
     1081 aacatggcgc atggcgagct gatgatgatc ggcgcctacg ccacctggct ggtgcagaac
     1141 ctgttccggc actacgcgcc gcactggttc gacgcctacc tgatcctcgc gattcccgtg
     1201 tccttcctcg tcgcggcgct ggtcggcgcg gtgctggaac ggggcgtgat ccgctttctc
     1261 tacggccggc cgctggaaac gcttcttgcg acctggggca tcagcctcgt gctgatgcag
     1321 ctcgtgcgca cgctcttcgg cgcgcagaac gtcgaagtcg agaacccgtc gtggatgtcc
     1381 ggcggcatcg cggtcgcgag caatctcgtg ctgccctgga accggctcgc gatcatcgtc
     1441 ttcgcattcc tcgtgctcgc cgccaccgga ctgttgatca gccggacccg actggggctg
     1501 ttcgtgcgcg gcgtcacgca gaaccgccgc atggccgcgt gcaccggcat cgacacggcg
     1561 cgcaccgaca cgatggcgtt cgcgctgggc gccggcatcg ccggcctggc cggctgcgcg
     1621 ctgagccagg tcggcaacgt cgggcccgac ctcgggcagg gctacatcgt cgacgccttc
     1681 atggtcgtgg tgctgggcgg cgtcggacag ctgaccggca cggtgctggc ggccctgggc
     1741 ctgggcatga tgaacaaggc gctcgaaggc atcgccggtg ccgtgctggc caagatcctc
     1801 gtgctcgtct tcatcgtcgt gttcatccag aaacgtccgc agggcctgtt cgccctcaag
     1861 ggccggagcg ccgagtcatg agtcccgagg tgaagcccgt gcggcccgtg cacctgctgc
     1921 ccgaggcgcg tcgtggcctg cttctcggcg cgcgcggctg gacggccgtg gcgggaacgc
     1981 tgctgctggt cgcggcggtc gtgcccgtgc tcaacctgct cgtgccctcg ggccacatgc
     2041 tgcatctggg cgactggggc gtcgcgctca tcgccaagat catgtgctac gcgatctgcg
     2101 cgctggcgat ggacctgatc tggggctaca ccggcattct ctcgctgggc cacggcctgt
     2161 acttcgcgct gggcggctac gcgatgggga tgtatctcat gcgccagatc ggccacgacg
     2221 gcaactatcg cagcgacctg cccgacttca tggtgttcct ggactggaag gagttgccct
     2281 ggacctgggc gggcagcgcc tcgctcgcgt tccagctggt cctcgtggtc gcggtgcccg
     2341 cgctggtcgc gggtgtgttc ggctggttcg cgttccgctc gcgcatcaag ggcgtgtact
     2401 tctcgatcat cacgcaggcc ctgaccttcg ccgcgatgct gctgttcttc cgcaacgaga
     2461 cgggcttcgg cggcaacaac ggcttcaccg acttcaagcg catcgcgggc gtgccgatcg
     2521 cgttgccgtc gacgcgcatg gcgctgttcg tcgtcaccgg ctgcacgctg ctgggcttct
     2581 ggctgcttgc ccgtgcgatc gtcaactcga agttcgggcg cgtgctgcag gccatccgcg
     2641 acgccgagag ccgggtgatg ttcaccggct acgacccgct gcgctacaag ctggcgatct
     2701 gggtgctgtc ggccgccatg tgcggcgtcg ccggcgcgct gtacgtgtcg caggtgggca
     2761 tcatcaaccc gagcgagatg tcgccctcgg cgtcgatcga gatggccgtc tggtgcgccg
     2821 tcggcggacg cggcacgctg gtgggcccca tcctcggcgc gttcttcgtc aatggcgcca
     2881 agagctggtt cacccaggcc tttcccgagt tctggctgta tttcctcggc gcgctgttca
     2941 tcgcggtgac cctgttcctg ccgcgtggcc tgatgggcat cgcggcgagc ctgcggcgcg
     3001 agaagggcat ggaggagacg cggtgacgcc ggaactgatg gagcagggca tcgccgtcct
     3061 cgagcgcgcg catgcgcgag aggcggccgc gccctcgggc ggcgcgagct tcggccgcgt
     3121 gctgcagcct cgcgtgcccg acttcgcgca cggcgtggcg ctgtacctgg atgcgatcag
     3181 cgtcagcttc gacgggttca aggcgctcaa cgagctgtcc ctggcgatcg acgtcggcga
     3241 gctgcgttgc atcatcggcc ccaacggcgc cggcaagacg acgatgatgg acgtcgtcac
     3301 cggcaagacc cgtcccgacg ccggccatgc gcacttcggc agcaacatcg accttttgcg
     3361 actgcgcgaa cccgagatcg tgcgcgccgg catctgccgc aagttccaga agccgaccgt
     3421 ctacgagcag ttgtccgtgt tcgagaacct ggagctggcg ctggcggccg accggggtgt
     3481 gcgcgcggcg ctgacggcgc gcgtgacgcc gctgcagcgc gagcgcatcg ccgagatcct
     3541 ggaggtggtt catctgctgc ctgaggcgag acgcatcgcg ggattgctgt cgcacgggca
     3601 gaagcagtgg ctggagatcg gcatgttgct ggcgcaggag cctgtgctgc tgctgctgga
     3661 cgaaccggtc gcgggcatga ccgacgagga aaccgaacgc acggccgagc tcttcctggc
     3721 gctcgccggc aagcattcgc tggtggtggt cgagcacgac atgaagttcg tcgggatgct
     3781 gacgcagggc catcgcaagg tgacggtgct gcacgagggt gcggtgctcg cggagggcac
     3841 gctcgcggac gtgcaggcca atccgcaggt gatcgacgtc tacctgggac gatagatgct
     3901 cgaggtcgaa tccctcaatc aatactacgg cggctcgcac atcctgcgcg atgtctcgtt
     3961 cgaggcgcgg gccggcgaag tcacggtcat cctcggccgc aacggcgtgg gcaagacgac
     4021 cctgctcaag agcctgatgg gggtcgtcgc cgtgcgtgcc ggctcgatcc ggctcggcgg
     4081 acacgagctc gcgggactcg ccccctacga gcgggtgcgc cgcggcgccg gttacgtgcc
     4141 ccagggccgc gagatcttcg gccgcctgac ggtcatggac aacctgcgaa tgggactggc
     4201 ctcgcggccg gcacgggagg acgtgcccgc cgagctgttc gacctgttcc cggtgctggc
     4261 gcagatgcgc gaccggcgcg gcggcgatct ctcgggtggc cagcagcagc aactggcgat
     4321 cgcgcgcgcg ctcgcgccgg ggcccgacct gctcctgctg gacgagccca ccgaggggat
     4381 ccagccgtcg gtcatcaagg acatcggacg cgtgatccgc aggctggccg accgcaagac
     4441 gatggccatc gtgctcgtgg agcagtacta cgacttcgcc gcggaactgg ccgatcgcta
     4501 catcgtgatg gagcggggcg agatcgtcgc tcgcggagat ggttccacga tggcggcgga
     4561 cggggtgagg gcattgatgt ccgtgtgaac gatgcgagca cgtcggccgc gcccgcacgt
     4621 cccgtcagcc gctcatgtac gccagacccg cggggggcag ggcgccagcg tccatgcgaa
     4681 cgtgcgccag cgcttccaga tctgcgtcat cagctcgaac gcgggctcga tgcgcggcgc
     4741 gagcatgcgc acgaccacca cttcggcctg gggggccgtc gcgcccaccg tggctgcgag
     4801 cgaatgggcc gcggcgacat cgcgcgcgat gtggagcagc gcctcgcggc gctcgtcgga
     4861 catctgcgca ccggccgcga accagagggt gccgagcgcg cgatggccgg cccagccgca
     4921 ggggccgtcc agcaacgcca ggtcggtggc ggccgtgcgc ccgcgttcca gccagcgtgg
     4981 ccggccggcg gccgacaagg cgatgtcatg ccggtagtcg ccgcggagga agggcgcttc
     5041 ggcggcgggc aggcccaggg cgacgcagtc ccagccgatc atctcggcgc ccggcgccag
     5101 gtcgaagcgc agccggttgt ccgccagcgc gcccgagtgc accagggttt cgagcggcag
     5161 ccactcgagc cgtgcaccgt cggcgacgcg tgcgtcgacg acctgggccg cccgcgctcc
     5221 gctgctgcgg tagaagcgcg ttgcgcccgg cgtcgtcagc agtgcgtgcg ttcgcggtgc
     5281 gagtccgatc gacaggtcca acgtgtcgcc gccgacgatg ccgccgggtg gatgcacgat
     5341 cacggaatgg cagacggcct cgccctcggg atgcaggctc ttgaggatgc gcagcggccc
     5401 gtcatggcgg aagtcgacgc aggtgcgcgt cgagccggcg ctgccgttgg aatccgttcg
     5461 atagtccagg tcgagacgag cgtgccaggc catgcgatga gcgatcggtc agtaggaaca
     5521 gatgctcagc gtaccctagt gtcgagcctg tcccgtcaac gctcgagcag ctcgactcca
     5581 cgcgcctcca gccgcaggct gccgtcccag cgctggccac cgagcacgcc tttcatcgcg
     5641 ccgtcgatcg ccacgtcgac cggcgcattg ttggtgttca cataaagaac gcgtccatcg
     5701 acggcgcgtg cgaatacgcc gtcaggcgtc ctggggccgg gttcgatgcc gagctgcgcg
     5761 tacagctggc ggtagagggg ccgcatgatc tgcggctgtg cgggcgtcgc gacgtagatc
     5821 gcgcgcccct tgccgaagcg gttgacggtg atcgccggcg gcatcccctc gaggttgctg
     5881 aagcggctca tcacctgggc ggtggacggc tccaatactt catagtgctc gatcgtgccc
     5941 tcgacctgct cgccgccgat ctcgagcgtg ccggcggcgt cgtagaactc gttggtgcgc
     6001 aggccgaaga catcggtcag gcccccggga agcggcgtgg cgtgccactg gtcgtggccg
     6061 tccaccttgg ccgattgcgc cgtcatgacc acggtgccgc catcggcgac gaacttgcgc
     6121 agcgcgtccg tggaggcctc gtcgagcagg tacatgcccg gcaccacgat cagtcggtag
     6181 cggctcagat cctcgtacga cagcttgatg accgcgacgt cgacgttgtc cttgagcagc
     6241 ggctcgaagg cgccgtgggc ctggaccaga tagggcgtct tcaggtactg gcgcgaggtg
     6301 ttgctgggac ccggggcgct gtacgccgcg gtgttctcga acgagtacgc gatggccacc
     6361 ttgggctcga ccttgcgagg gaagccgagc ttctcgagcc tggcgtagtc gctggcgatg
     6421 cgggcccact ccgcgagctt ccatgaaggg cggtcgtcat ggtcgatgag ccccatcaac
     6481 gcctgctcct cgccgccgag gtggctgttg aacgtccagg cgaccgcggc ctggccgccc
     6541 atcaagagca cgaagtgggc ccacatgcgc gaccagcccc tgctgccgta ggaaccgccg
     6601 ccgccggcgg cgaattcgtt gaaccaggtc ggcgtgctca tgccgccctt gatgagcgct
     6661 gcctgccagc cgccgtccag ggcgtcgccg gcatagaacc ccatcgcgcc gtaggtggtg
     6721 tgccgccgat agctgcccag gtagtcgaag cctttgcggt ccgcccagtc ccagtagttg
     6781 gaaagcgtgg gcgtgtcggg cgtgttcttc ttgcgcacgg cctccaggtc ggtgagggtc
     6841 gcgatcgtga tgtccgacca gaagcgccgc aggtcgaggt agcgctcggc cgggccaggt
     6901 ccgtcgccat agggaagtcg gacctcatcc cactggccga tgcggcgcga ccagcgctgc
     6961 gtggcccagg ccttgttcaa cgcctcgagg ctgccgtacc gcttcttcag ccaggcgatg
     7021 aagcgcttgc ggtcggcttc ggagtacgac atgaacccgt tgccgatctc gttgtcgtac
     7081 cccacggcca gcagcgccgg gtgccgggcg tagcgccggg tcagcgtgtc ggccatgcgg
     7141 atggccaggc ggcggtagtc cgggtcgccg atatcgtcca tgtagcgctc ggccggtgcc
     7201 aggcgcgtgc cagcctggtt gacgatgtcg acgcccgggt acttgtggtg cagccacgtc
     7261 ggcgccggaa ggccgggaat gtcgaggatg accttgatgc cggcggcctg catctcgttc
     7321 atcaccgcgt cgaagtgacg gaagtcgaac cggccctcct gcggctcgaa gtagtcccag
     7381 gacaggtcgc ccattcgcac gaccttgaag ccggcctgct tcatcagggc gacgtcgcgc
     7441 ttcacctgtt ccaggctgcg gtccaccggc tggtagcagg taccgacgaa cagttgccct
     7501 gcgcccggcc agtccggatg gccgtcggcg ccctgggcca ggacggtttg aagcgtggcc
     7561 agcgcgagca ggcaggccgc cagggtcttc ttcattggct tggtgatgaa catggtgtgc
     7621 ctgagctcat gcgatgccgc gatgcggcgc tgagcgtggg ccgactcggc ggctcacgtc
     7681 cgtttcgccg agtgcgtgcg cgcggggcgc ctgggcggcc attgcgcttt cccctcggca
     7741 tcgagggcga gcgtcatcag gccgcccgcg tgtcgcacgc gcagtacggc gccggggcgg
     7801 ccccgcaacg ccgcgcgggt caggcggcct ttcgaccatg ccaggtcgat ttccacgccg
     7861 ccgcgggcgc gcacgccccg cagttcgcct tcgggccacg cggccgggag tgccgggagc
     7921 agcctgatct cgccgcccca ggactgcacc agcatttcga gcatgcccgc ggcgccgccg
     7981 aaattgccgt cgatctggaa cggcggatgg gcgtcgaaca ggttgggata ggttctttcc
     8041 gtgctcagca gcccctgcag gatgcccagc gcgcgctggc cgtcgcccag gcgcgcccac
     8101 agcgccagcc gccaagccgt cgcccatccc gtggacaggt cacctcgggt gttcagcgtc
     8161 gtcttggccg cgcgcaccag cgccggcgtg tctcgaacgt tgatctgatc gctggggaac
     8221 aagccataga gatgcgagac atggcgatgc cgctggtccg gcgcctgcgc atcccaatcg
     8281 tccagccact cctggagctg gccctgcgcg ccgatccggt cggccggcag ccgggcgcgt
     8341 gcgcgactca cggactcggc aaacgcgtcg tccgcgacgc cacagcgccg ctgcgcatcg
     8401 agcacggcgg caaacaggtc gcgcaggatc tgcctgtcca tggccggccc ggcgcagatc
     8461 gccacgccag ccccatgcgc gttctccggc gagatcgacg gtgacgtcac caacccgcgc
     8521 ccctcgggat cctccaccag cgtatcgagg aagaattcgg ccgcgcctcg catcagcggg
     8581 tagaggcgcc tcaacagcgc gtcgtcctgc ccgtagtgga attcgtccca gagggacagg
     8641 cacagccacg cgccgcccat cggccagagg ccccacatcg gcccgtcgat cggcgccgtg
     8701 gcccgccaca gatcggtgtt gtgatgggcc acccagcccc tggcgccgta ttgcagccgc
     8761 gccgttcttg cgccggtgac ggccaggtcc tcgaccatcc gcagcagggg ctcgacgcac
     8821 tcgtgcaggt tggccggacc tgccagccag tagttcatct cggtgttgat gttgatcgtg
     8881 tacttgctgc cccagggcgg gttgttgccc tcgttccaga gaccctgcag gttggccggc
     8941 tggccgccgg gccgcgagga ggagatgagc aggtagcgcg cgtagttcag gtagagcacg
     9001 gcgaagtccg gctccagcgg gtccgaaggc ggacggattc gccggtccgt ggggggcgcc
     9061 gctgccgagg gcttgcccag ccgaagcgtc attcgacgat acagcgcccg gtggctcgcc
     9121 aggtgctcgg ccatcagcgt gtccaggccc ttcgacgcgg cgacttccgt gcgatcgcgt
     9181 accgcggcca ccgggtctcc actcacgtcg tcgaaccgtc ggtagctcgt ctcgccggca
     9241 accagcagca acgcatggcg ggcgccgcgg atgctcagcc ggtccccctc gacgaccacc
     9301 tggccgtcgg tgacgacacg cacgcgcacg gcgtagc
//
LOCUS       DATCRO010000529        10805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_39474, whole genome shotgun sequence.
ACCESSION   DATCRO010000529 DATCRO010000000
VERSION     DATCRO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39474"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..2099
                     /gene="recG"
                     /locus_tag="VIN75_14495"
     CDS             57..2099
                     /gene="recG"
                     /locus_tag="VIN75_14495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8975423.1"
                     /translation="MDKLGLVRDIDLALHLPLRYEDETQVTPIARAYPGQKVQVEGVV
                     LESRVELRGRRQFVVKMTDESDAELVLRFLNFYPSHQKTFAPGQRVRVRGEIRGGFLG
                     REMVHPTFKAVVEGAPLASSLTPVYPTTAQLPQPYLRKAIAGSLGRAPLQEVLPPQVV
                     PAGLPSLRVALETLHHPPPDVSLHALEDGSHPAAQRLKFEELLAQQISQLAAKAERAL
                     LRGPAIVPRPGGLHEKLLGVLPFGLTDAQRRVVDEITADLAGPRPMHRLLQGDVGAGK
                     TVVAALAAAVAIDAGWQCALMAPTEILAEQHFAKLLQWLEPLGIEVAWLSGSRKGKAR
                     AQALEKAASGAARLIVGTHAVIQEGVQFARLGLAIVDEQHRFGVQQRLALRRKLAIEG
                     LEPHLLMMTATPIPRTLAMTYFADLDVSTIDQLPPGRTPIVTRVFADTRRPDIVERIR
                     DDIAAGRQVYWVCPLIEESEDAENAAAEFAGKSEALTLQNVTDTHAALEQALPGIGVG
                     LLHGRMKPADKAAVMSRFKSGELPVLVATTVIEVGVDVPNASLMVIEHAERFGLAQLH
                     QLRGRVGRGSAASVCVLLYAGPLSQIGKERLKAMAETTDGFEIARRDLQIRGPGEFMG
                     ARQSGDQLLRFADLEEDAPLLAHVREVAPQLLARAPGAASAHVERWLGGRLEWMKA"
     gene            2304..3242
                     /locus_tag="VIN75_14500"
     CDS             2304..3242
                     /locus_tag="VIN75_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8975424.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEEELD
                     VKIFERGSNEVSLTALGTAIVRQAQSVIEQAAAIKEIAKSGQDPLSGALKLGIIYTIG
                     PYLLPQLVRHAIELTPQMPLMLQENFTVKLLDMLRTGELDCAIMAEPFPDSGLAIAPL
                     YDEPFVVAVPRSHALAKEKSITSERLKQETMLLLGTGHCFRDHVLEVCPEFARFSNDA
                     EGIRKSFEGSSLETIKHMVASGMGVTVVPQLSVPQGLEDPQSHALVTYIPFEPPVPTR
                     RVVLAWRRSFPRYEAIAALRNAVYACALDGVTRLSD"
     gene            3265..7053
                     /locus_tag="VIN75_14505"
     CDS             3265..7053
                     /locus_tag="VIN75_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8975425.1"
                     /translation="MDARFARRARGTLAALSFALPPIVAGATPELPTTIREAVSAPAV
                     AAPASAPRVLRVVGADNYPPYLFRDEDGRPAGLVADEWALWEKKTGVHVDLQPVDWAD
                     ALRRVADGQADVIDTIFWSSDRARTMDFTAPFADVREPIYADASVKGLVDLHSLRGFH
                     VAALAGDVCIDRLHAGGVTQIDTFPSYQSLIASAVAGGPKIFCMDGPSAEYYLYKANA
                     SQRFREAFVMYYGHMHRAVRRGDAATLALIEKGFDQVSPTEKKAAEDKWFGAPLAGAR
                     WLHLALVAAGVLALAALLALLWGLFLRRAVREKTAEIDSQARRLRTLIDTLPDLVWFK
                     GVDGRFLACNVRFERFIGAPENELIGHAASAFLPAAVAAEFAAADKRAIDMQAPVPHQ
                     FTLTYAHDGHQEQVESLHTPVFGSHGELAGVLSIGRDVTQRRDDERHLRRLNRLYQVL
                     TNVHAAIARERAPEPLLAAVCQIMVRDGGLRMAWIGRPDAEAGELVPVAWAGKTGDYL
                     DPPHATLAAGPRGHAPSAQAFREGRAMHSTDIGSDPGMVTWRESALALGYRSSSAYPL
                     KSRGRVVGVFTMYSSAIQFFDAGELALLERLSEDIGHALESYELAEERERAVTDLRDS
                     EARFATMFRMSPSGLGLARFEDKRYIDVNEAFIQMFGYSWEEAHNRSGLELNTWVDVK
                     LRGEAMATLEREGEVRNVEAPFRTRSGDIFYASFSAMRLSIGREDLVVASYFDLTAQR
                     LAARTLEERKLELERIVSQRTAELSRILDAMPDRYFRIRQDGTVVDFRMGRHEDSYAD
                     TSTLLGRRIGDVLPAAAAEPLLRAVSEAIASRSLVMVEYALPYPDGERHFEARTQALG
                     TDQVIMFVRNITDRHSLEADRERARLNAVALANAKSEFLANMSHEIRTPLNGLLGLAQ
                     IGQRETAGSRTAATFAAILNSGRLLLGIVNDVLDFSKIEVGKLRVEQRPISPHALAEE
                     AVALLRDRADEKGIALNLAFDPALPDRCLGDALRTQQVLVNLLSNAIKFTETGHVTVW
                     AGMEPGPAAADPCMVYRVTDTGIGIATEHLKHLFDAFHQADTSTTRRFGGTGLGLAIS
                     KRLAELMGGAVHVSSELGAGSVFELRLPWLAATEMPQEHEPRTSWGDQPRRLQGVRVL
                     VAEDNEVNQLVLERALEIEGAATTIVGDGRQAVNRVLADGRESYDVVLMDIQMPEMDG
                     YEATRRILELAPDLPVIGQTAHAMLDERRECLAAGMVDHIAKPLDFRLLADMIVRHIG
                     "
     gene            7316..8032
                     /locus_tag="VIN75_14510"
     CDS             7316..8032
                     /locus_tag="VIN75_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975426.1"
                     /translation="MTDNTQSDDKPGLGAQLWGAVRGALVEEDPVAATRRAKLQGGHA
                     RADKTDPAPAPAAVPAPMSAMATTLLAQVLARPTAYTALTEKLEPLEGIVLDERTRYQ
                     AAYALIRNSRSVEQLVQAIDMQHAQALEDEKTRFAAQLQDKDRSEIGARSQELQVLDA
                     RMDAAARQVATLREETDARIAQIEASVARDRERQALLAREVEDKRQELAAVQRQFDAA
                     AGSVAQALTTAKATLLRHLG"
     gene            8089..9129
                     /locus_tag="VIN75_14515"
     CDS             8089..9129
                     /locus_tag="VIN75_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975427.1"
                     /translation="MSDIIPTDYKPLSFWERKEGVTGQVVGVALLCGAGVLLYRALPT
                     IITLLENTIYAAVLGVAAVALAFVITDRRFWRLGAWAYMSAMRRITQVFVEIDPIGIM
                     KNYIEDLKRKLADMNGRISRLSGMIRACKEEIRQNDQAKTGALGLVSEAKKKGLTMVA
                     AAQSRKAGRIAEANLTYQDLLAKMELLHRVLVKYQEVSTFLIEDMTQEVVIKERKRAM
                     AKEAYSAMKSAMAILHGDRNARELYDMASEYVAADYAMRIGEIEDFVRMSDNFMQSID
                     LRNGVYEQQAVEMLADWEKNADSIVLGDAKRLLIENHPDAVARDTRAGTPRTRGHGND
                     TPDLDWFDQAGQ"
     gene            9160..>10805
                     /locus_tag="VIN75_14520"
     CDS             9160..>10805
                     /locus_tag="VIN75_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8975428.1"
                     /translation="MAIKPLPRLILIAAAVGGAWYGLDRFAHRPRDIAASAVPGQADV
                     PVATAAAATAQPTLVTSTLAPATGDYTARTLVLPWNAVAGLAYANGDVNTAADSLMAR
                     HGVHLELERQDDYAQMVAEQALFASEVANGVARPAKGAAFVVIMGDALPGYAASAKAA
                     LDKLGQQVEVVGALGYSRGEDKCMLPAAVASDPQKARGSLIGAVLRDGDWNICIKYAA
                     DNRIPVNADPKTYDPDAMNFVSVGAFTEADEKLIAASKGACETRPVVSAGKLTGESRR
                     VCQDGTATWTPGDVKVAREVGGLAAVASTREYMWQMPAVIIGNKQWMAQNPAFVKNLL
                     AASFEASDAIKGSDANLLAASTVMSKVMKEQTPQWWAKYHRGVVEKDAKGQDIRLGGS
                     TTNNLADNLYLFGLNGNDNLYKRVYTVYGGIARSMYPQEMPQVLPYESVVDAGYVQSL
                     AAEATGAAQARPATPVFDAQAPATSTFAKRSWAIEFDTGKATFRPEAIPVLDQMLDQV
                     SVSGLVVQVSGHTDNVGDPSSNLALSRRRAEAVSAWLQANA"
BASE COUNT         1707 a         3873 c         3782 g         1443 t
ORIGIN      
        1 tgtcagcgca gcccacggaa ggtacgacgg cgacgaagtc cgggccgcag aaggccctgg
       61 acaagctcgg cctcgtgcgc gacatcgacc ttgcgctgca cctgccgctg cgctacgagg
      121 acgagacgca ggtcacgccc atcgcccgcg cctacccggg ccagaaggtg caggtcgagg
      181 gcgtcgtgct cgagtcgcgc gtggagctgc gcggccgccg gcagttcgtc gtgaagatga
      241 ccgacgagtc cgacgccgag ctcgtgctgc gcttcctcaa cttctacccc tcgcaccaga
      301 agaccttcgc gcccggccag cgcgtgcgcg tgcgcggcga gatccgcggc ggcttcctcg
      361 gccgcgagat ggtgcacccc accttcaagg cggtggtcga gggggcgccg ctcgcgtcgt
      421 cgctgacgcc ggtctacccg accaccgcgc agctgccgca gccctacctg cgcaaggcga
      481 tcgccggcag cctgggccgc gcgccgctgc aggaggtgct gccgccgcag gtggtgccgg
      541 ccgggctgcc gtcgctgcgc gtggcgctgg agacgctgca ccatccgccg ccggacgtct
      601 ccctgcacgc gctcgaggac ggcagccacc ccgccgccca acgcctgaag ttcgaggagc
      661 tgctcgcgca gcagatctcc cagctcgccg ccaaggccga gcgcgcgctg ctgcgcggcc
      721 cggcgatcgt tccgcgcccg ggcggcctgc acgagaagct gctgggcgtg ctgccgttcg
      781 gcctgacgga cgcccagcgc cgcgtggtcg acgagatcac cgcggacctc gccggcccgc
      841 ggccgatgca ccggctgctg cagggcgacg tgggcgccgg caagacggtg gtggccgcac
      901 tggccgcagc cgtcgcgatc gacgccggct ggcagtgcgc gctgatggcg cccaccgaga
      961 tcctggccga gcagcacttc gccaagctgc tgcagtggct ggagccgctg ggcatcgagg
     1021 tggcctggct cagcggcagc cgcaagggca aggcgcgggc gcaggcgctc gagaaggctg
     1081 ccagcggcgc cgccaggctg atcgtcggca cccacgcggt catccaggaa ggcgtccagt
     1141 tcgcacgcct gggcctggcc atcgtcgacg agcagcaccg cttcggcgtg cagcagcggc
     1201 tggcgctgcg gcgcaagctg gccatcgagg ggctggagcc gcacctgctc atgatgacgg
     1261 ccacgccgat cccgcgcacg ctggccatga cctacttcgc cgacctggac gtcagcacca
     1321 tcgaccagct gccgcccggt cgcacgccca tcgtcacgcg cgtgttcgcg gacacgcggc
     1381 ggcccgacat cgtcgagcgc atccgcgacg acatcgccgc cggccggcag gtctactggg
     1441 tctgcccgct gatcgaggag tccgaggacg ccgagaacgc ggcggcagag ttcgccggca
     1501 agtccgaggc gctgacgctg cagaacgtca cggacaccca cgctgcgctg gaacaggcgc
     1561 tgccgggcat cggcgtcggc ctgctgcacg ggcgcatgaa gccggccgac aaggcggcgg
     1621 tgatgagccg cttcaagtcc ggcgagctcc cggtgctggt ggccacgacc gtcatcgagg
     1681 tgggcgtgga cgtgcccaac gccagcctga tggtgatcga gcacgccgag cgcttcgggc
     1741 tggcgcagct gcaccagttg cgtggccggg tggggcgcgg cagcgctgcc agcgtgtgcg
     1801 tgctgctgta cgccgggccg ctgtcgcaga tcggcaagga gcggctcaag gcgatggccg
     1861 agacgaccga cggcttcgag atcgcccggc gcgacctgca gatccgcggc ccgggcgagt
     1921 tcatgggcgc gcgccagtcc ggcgaccagc tgctgcgttt cgccgacctg gaggaggatg
     1981 cgccgctgct cgcgcatgtt cgggaggtcg cgccgcagtt gctggcgcgg gcgcccgggg
     2041 ccgcatcggc ccacgtcgaa cgctggctgg gcggccggct cgagtggatg aaggcctgat
     2101 cgagagctgc ggtcaaccct ggcaactcca tagcgcacaa agctatggac agcggccaca
     2161 atagagggga tgctatctcg tcccggcgct gccgctcgaa cgtaccgaca ggagttcgag
     2221 ggagcgacgg atccggcttc gccgggccgt tcgcggccgg cccctcgggg gcagcgagcg
     2281 ctcgcgagcg tggggcccaa tttatgacac tcaccgagct gcgctacatc gtcgcggtcg
     2341 cccgcgagcg gcacttcggc cgcgcggccg aggcgtgctt cgtgtcgcaa ccgacgctct
     2401 cggtggcgat caagaagctc gaggaggagc tcgacgtcaa gatcttcgag cggggcagca
     2461 acgaggtgag cctgacagcg ctgggcaccg ccatcgtgcg gcaggcgcag agcgtcatcg
     2521 agcaggcggc cgcgatcaag gagatcgcca agagcgggca ggatccgctg tccggcgcgc
     2581 tcaagctggg catcatctac accatcggcc cgtacctgct gccgcagctg gtgcgccacg
     2641 ccatcgagct gacgccacag atgccgttga tgctgcagga gaacttcacc gtcaagctgc
     2701 tggacatgct gcgcaccggc gagctcgatt gcgccatcat ggccgagccc ttcccggaca
     2761 gcggcctggc catcgcgccc ctgtatgacg agcccttcgt cgtggcggtg ccgcgctcgc
     2821 acgcgctcgc caaggagaag tcgatcacct cggaacgact gaagcaggag acgatgctgc
     2881 tgctgggcac gggccactgc ttccgcgacc acgtgctgga ggtgtgcccc gagttcgcgc
     2941 gcttctccaa cgacgcggaa ggcatccgca agagcttcga gggctcgtcg ctggagacca
     3001 tcaagcacat ggtcgcctcg ggcatgggcg tgacggtggt gccgcagctg tcggtgccgc
     3061 agggcctgga agatccgcaa tcgcatgcgc tggtcaccta catcccgttc gagccgccgg
     3121 tgcccacgcg gcgcgtcgtg ctcgcgtggc ggcgcagctt tccccgctac gaggccatcg
     3181 ccgcactgcg caatgccgtc tacgcctgtg cgctggacgg cgtgacgcga ctgtcggact
     3241 agcgccctgg cacccgccat cgtcatggac gcccgattcg cacggcgtgc gcgcggcacg
     3301 ctggcggcgt tgtccttcgc gctgccgccg atcgtcgccg gcgccacgcc ggagctgccc
     3361 acgacgatcc gcgaggcggt ctccgcgccc gccgtcgccg cgcccgcctc ggcgccgcgg
     3421 gtgctgcgcg tggtgggcgc cgacaactac ccgccctacc tgttccgcga cgaggacggc
     3481 cgccccgcgg gcctggtcgc cgacgaatgg gcgctgtggg agaagaagac cggcgtgcac
     3541 gtcgacctgc agccggtgga ctgggccgac gcgctgcgcc gcgtggccga cgggcaggcc
     3601 gacgtcatcg ataccatctt ctggtcgtcc gaccgcgccc gcacgatgga cttcaccgcg
     3661 ccgttcgccg acgtgcgcga gcccatctac gccgacgcca gcgtcaaggg cctggtcgac
     3721 ctgcactccc tgcgcggctt ccacgtcgcg gcgctcgctg gcgacgtctg catcgaccgg
     3781 ctgcatgccg gcggcgtcac gcagatcgac accttcccga gctaccagag cctgatcgcg
     3841 tcggcggtgg cgggcggccc gaagatcttc tgcatggacg gcccgtccgc cgagtactac
     3901 ctgtacaagg ccaacgcctc gcagcgcttt cgcgaggcct tcgtcatgta ctacggccac
     3961 atgcaccgcg cggtgcgccg cggcgacgcc gcgacgctgg cgctgatcga gaagggcttc
     4021 gaccaggtct cgccgaccga gaagaaggcc gccgaggaca agtggttcgg cgcgccgctg
     4081 gccggcgcgc gctggctgca cctggcgctg gtggccgccg gcgtgctcgc gctggccgcc
     4141 ttgctcgcgc ttctgtgggg cctgttcctg cgccgcgcgg tgcgcgagaa gaccgccgag
     4201 atcgacagcc aggcgcggcg cctgcgcacg ctgatcgaca cgctgcccga cctcgtctgg
     4261 ttcaagggcg tggacggcag gttcctcgcg tgcaacgtgc gcttcgagcg cttcatcggc
     4321 gcgcccgaga acgagctgat cggccacgcg gccagcgcct tcctgccggc cgcggtggcc
     4381 gcggagttcg ccgccgccga caagcgcgcg atcgacatgc aggccccggt gccgcaccag
     4441 ttcacgctga cctacgcgca cgacggccac caggagcagg tcgagtcgct gcacacgccg
     4501 gtgttcggct cgcacggcga gcttgccggc gtgctgagca tcggccgcga cgtcacgcag
     4561 cggcgcgacg acgagcgcca cctgcggcga ctgaaccgcc tctaccaggt gctgacgaac
     4621 gtgcacgcgg ccatcgcgcg cgagcgcgcg cccgagccgc tgctggccgc cgtgtgccag
     4681 atcatggtgc gcgacggcgg cctgcgcatg gcctggatcg gccggcccga cgccgaggcc
     4741 ggcgagctgg tgcccgtcgc ctgggccggc aagaccggcg actacctcga tccgccgcac
     4801 gccacgctcg ccgccggacc gcgcgggcac gcgccgagcg cgcaggcgtt ccgcgagggg
     4861 cgcgcgatgc actcgaccga catcggctcc gaccccggca tggtcacctg gcgcgaaagc
     4921 gcgctggcgc tcggctaccg ctcgtcgtcg gcgtatccgc tgaagtcgcg cggccgtgtc
     4981 gtgggcgtgt tcacgatgta ctcgtcggcg atccagttct tcgacgcggg cgagctcgcg
     5041 ctgctggagc gcctgtccga ggacatcggc cacgcgctcg agagctacga gctcgccgag
     5101 gagcgcgagc gcgccgtcac cgacctgcgc gacagcgagg cccgtttcgc cacgatgttc
     5161 cgcatgagcc cgtcgggcct gggcctggcg cgcttcgagg acaagcgcta catcgacgtc
     5221 aacgaggcct tcatccagat gttcggctac tcgtgggagg aggcgcacaa ccgcagcggg
     5281 ctggagctca acacctgggt cgacgtcaag ctgcgcggcg aggcgatggc cacgctggag
     5341 cgcgagggcg aggtgcgcaa cgtcgaggcg ccgttccgca cgcgctcggg cgacatcttc
     5401 tacgcgtcgt tctcggcgat gcggctgtcg atcggccgcg aggacctggt ggtggcctcg
     5461 tacttcgacc tgaccgcgca gcgactggcc gcgcgcacgc tggaggagcg caagctcgag
     5521 ctcgagcgca tcgtgtcgca gcgcacggcc gaactcagcc gcatcctcga cgcgatgccg
     5581 gaccgctact tccgcatccg ccaggacggc acggtcgtcg acttccgcat gggccgccac
     5641 gaggacagct acgccgacac gagcacgctg ctcgggcgcc gcatcggcga cgtgctgcct
     5701 gcggcggcgg cggagccgct gctgcgcgcg gtctccgagg ccatcgcgag caggagcctg
     5761 gtgatggtcg aatacgcctt gccgtatccg gacggcgagc ggcacttcga agcgcgcacg
     5821 caggccctgg gcaccgacca ggtcatcatg ttcgtgcgca acatcaccga ccgccactcg
     5881 ctggaggccg accgcgagcg cgcgcgactg aacgcggtgg cgctcgccaa cgccaagagc
     5941 gagttcctcg ccaacatgag ccacgagatc cgcacgccgc tcaacgggct gctgggcctg
     6001 gcccagatcg gccagcgcga gaccgccggc agccgcacgg cggccacgtt cgcggccatc
     6061 ctcaactcgg ggcggctgct gctgggcatc gtcaacgacg tgctcgactt ctcgaagatc
     6121 gaggtcggca agctgcgcgt cgaacagcgc ccgatctcgc cgcatgccct cgccgaggag
     6181 gccgtggcgc tgctgcgcga tcgcgccgac gagaagggca tcgcgctgaa cctcgcgttc
     6241 gaccccgcgc tgcccgatcg ctgcctgggc gacgccctgc gcacgcagca ggtgctggtc
     6301 aacctgttgt ccaacgcgat caagttcacc gagaccggcc acgtcacggt gtgggccggc
     6361 atggagcctg gcccggccgc cgccgacccc tgcatggtct accgcgtcac cgacaccggc
     6421 atcggcatcg ccaccgaaca cctgaaacac ctgttcgacg ccttccacca ggccgacacg
     6481 tccaccacgc gccgcttcgg cggcaccggc ctgggcctgg cgatcagcaa gcgcctggcc
     6541 gaactgatgg gcggggcggt gcacgtgagc agcgaactgg gcgccggcag cgtgttcgag
     6601 ctgcgcctgc cctggctcgc ggccaccgag atgccccagg agcacgagcc gcgcacgagc
     6661 tggggcgacc agccgcgccg cctgcagggc gtgcgcgtgc tcgtggccga ggacaacgag
     6721 gtcaaccagc tggtgctcga gcgcgcgctc gagatcgagg gcgccgccac gaccatcgtg
     6781 ggcgacggcc gccaggcggt caaccgcgtg ctggccgacg gccgcgagtc ctacgacgtc
     6841 gtgctgatgg acatccagat gcccgagatg gacggctacg aggccacgcg ccgcatcctg
     6901 gagctggcgc cggacctgcc ggtgatcggc cagacggcgc acgcgatgct cgacgagcgc
     6961 cgcgagtgcc tggccgccgg aatggtcgac cacatcgcca agccgctgga cttccggctg
     7021 ctggcggaca tgatcgtgcg gcatatcggc tgacgcgccg cgggcgctcg acgcgcgtga
     7081 cgtcacccgt cggcgaagcg cgtccctcaa cgagcgcgtt gccaccaaag cgcgtccacc
     7141 accgacgggc gtccaccacc gacgggcgtc cccaccgaat ggcggaggtg gcggaggtgg
     7201 agcatcggcc aagcaacgtc ctaacggaat cctgacgccg tcatatgcgg tccatacgac
     7261 cgccctggtc aagtcacgcc atgcgccgac acggcgcgct tcgtgatcga acaccatgac
     7321 ggacaacacg caatcggacg acaagcccgg cctgggcgcc cagctgtggg gcgccgtcag
     7381 gggcgccctg gtcgaggaag acccggtcgc ggccacgcgc cgcgccaagc tgcagggcgg
     7441 ccatgcccgt gccgacaaga ccgatcccgc acccgcgccc gccgccgtgc cggcgccgat
     7501 gtcggcgatg gccacgacgt tgctggccca ggtgctcgca cgcccgacgg cctacaccgc
     7561 gctgacggag aagctcgagc cgctcgaagg catcgtgctg gacgaacgca cgcgctacca
     7621 ggcggcctac gcgctgatcc gcaactcgcg cagcgtcgag cagctggtgc aggcgatcga
     7681 catgcagcac gcacaggcgc tcgaggacga gaagacgcgc tttgccgcgc aactgcagga
     7741 caaggatcgc agcgagatcg gcgcgcgctc gcaggagctg caggtgctcg acgcccgcat
     7801 ggacgccgcc gcgcgccagg tggcgacgct gcgcgaggag accgatgcgc gcatcgccca
     7861 gatcgaggcg agcgtcgcgc gcgatcgcga acgccaggcg ctgctcgcgc gcgaggtcga
     7921 ggacaagcgc caggagctcg ccgcggtgca gcgccagttc gacgcggccg cgggctcggt
     7981 ggcgcaggcc ctgaccaccg ccaaggcgac gctgctgcgc catctcggct gaacgccgtc
     8041 ccatttcagg caagacgacg ggccgtcatc ggcccaggag gaaagaccat gtccgacatc
     8101 atccccaccg actacaagcc gctcagcttc tgggagcgca aggaaggcgt caccggccag
     8161 gtggtcggcg tcgcgctcct gtgcggcgcc ggcgtgctgt tgtatcgcgc gctgcccacc
     8221 atcatcacgc tgctcgagaa cacgatctac gccgcggtgc tgggcgtggc cgcggtggcg
     8281 ctcgccttcg tcatcaccga ccgccgcttc tggcgcctgg gcgcctgggc ctacatgagc
     8341 gcgatgcgtc gcatcacgca ggtgttcgtc gagatcgatc ccatcgggat catgaagaac
     8401 tacatcgagg acctcaagcg caagctggcc gacatgaacg gccgcatctc gcggctgtcc
     8461 gggatgatcc gcgcctgcaa ggaggagatc cgccagaacg accaggccaa gactggcgcg
     8521 ctgggcctcg tgagcgaggc gaagaagaag ggcctgacga tggtggccgc cgcgcagtcg
     8581 cgcaaggccg gccgcatcgc ggaagcgaac ctgacctacc aggacctgct cgccaagatg
     8641 gagctgctcc atcgcgtgct ggtcaagtac caggaggtgt cgaccttcct catcgaggac
     8701 atgacgcagg aagtggtcat caaggagcgc aagcgcgcga tggccaagga agcgtactcc
     8761 gcgatgaagt cggcgatggc catcctccat ggcgaccgca acgcccgcga gctctacgac
     8821 atggccagcg agtacgtcgc ggccgactac gccatgcgca tcggcgagat cgaggacttc
     8881 gtgcgcatgt ccgacaactt catgcagtcg atcgacctgc gcaacggcgt gtacgaacag
     8941 caggccgtcg agatgctcgc cgactgggag aagaacgccg actcgatcgt gctgggcgac
     9001 gccaagcgcc tgctgatcga gaaccacccc gacgccgtcg cgcgcgacac gcgcgcgggc
     9061 acgccgcgca cgcgcggcca cggcaacgac acgccggacc tggactggtt cgaccaggcc
     9121 ggccagtaac gcgaacaacg aaccacgaaa ggaaccatca tggccatcaa gccgcttccc
     9181 cgcctcatcc tgatcgctgc cgccgtcggc ggcgcgtggt acgggctcga tcgcttcgcg
     9241 catcgcccgc gcgacatcgc cgccagcgcg gtgcccggcc aggccgacgt gcccgtcgcc
     9301 accgccgccg ccgccacggc gcagcccacg ctcgtcacgt cgacgctcgc gcccgccacc
     9361 ggcgactaca ccgcgcgcac gctggtgctg ccgtggaacg cggtggccgg ccttgcatac
     9421 gcgaacggcg acgtcaacac cgccgcggac agcctgatgg cgcgccatgg cgtgcacctc
     9481 gagctcgagc gccaggacga ctacgcgcag atggtggccg agcaggccct gttcgcgagc
     9541 gaggtcgcca acggcgtcgc gcgtccggcc aagggcgccg cgttcgtcgt catcatgggc
     9601 gacgcgctgc ccggctacgc cgcgtcggcc aaggcggcgc tcgacaagct gggccagcag
     9661 gtcgaggtcg tcggcgcgct gggctactcg cgcggcgagg acaagtgcat gctgcccgcc
     9721 gcggtcgcgt ccgacccgca gaaggcgcgc ggttcgctga tcggcgccgt gctgcgtgac
     9781 ggcgactgga acatctgcat caagtacgcg gccgacaaca ggattcccgt caacgcggat
     9841 ccgaagacgt acgacccgga cgcgatgaac ttcgtgtcgg tcggcgcgtt caccgaggcc
     9901 gacgagaagc tgatcgccgc cagcaagggc gcgtgcgaga cgcgtcccgt cgtgagcgcc
     9961 ggcaagctca ccggcgagag ccgccgcgtc tgccaggacg gcacggccac gtggacgccc
    10021 ggcgacgtga aggtggcgcg cgaggtggga ggcctcgcgg ccgtcgcgtc gacgcgcgag
    10081 tacatgtggc agatgcccgc cgtcatcatc ggcaacaagc agtggatggc gcagaacccg
    10141 gcgttcgtca agaacctgct ggccgcgtcg ttcgaggcga gcgacgcgat caagggcagc
    10201 gacgccaacc tgctcgcggc ctcgacggtg atgtccaagg tcatgaagga gcagacgccg
    10261 cagtggtggg cgaagtacca ccgcggcgtc gtggagaagg acgccaaggg ccaggacatc
    10321 cgcctcggcg gctcgaccac caacaacctg gcggacaacc tgtacctgtt cggcctgaac
    10381 ggcaacgaca acctgtacaa gcgcgtctac acggtctacg gcggcatcgc caggtcgatg
    10441 tacccgcagg agatgccgca ggtgctgccg tacgagtcgg tggtggacgc cggatacgtg
    10501 cagtcgctcg cggccgaggc cacgggcgcg gcgcaggcgc gtccggccac gccggtcttc
    10561 gacgcccagg cgcccgcgac gtcgaccttc gccaagcgct cgtgggccat cgagttcgac
    10621 accggcaagg ccacgttccg ccccgaggcg attcccgtcc tcgaccagat gctcgaccag
    10681 gtgtcggtgt cgggcctggt cgtgcaggtc tcgggccaca cggacaacgt cggcgacccg
    10741 tcgtccaacc tcgcgctgtc gcgccgccgc gccgaggccg tgagcgcctg gctgcaggcc
    10801 aacgc
//
LOCUS       DATCRO010000530         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_62094, whole genome shotgun sequence.
ACCESSION   DATCRO010000530 DATCRO010000000
VERSION     DATCRO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62094"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VIN75_14525"
     CDS             complement(<1..422)
                     /locus_tag="VIN75_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY8975429.1"
                     /translation="MDRRRFAHAAIALAGAWPVARALGGAEPAQKNWGDARAERWRAI
                     EAQVGGRLGVAMLDTATGELAGQRLDERFPMCSTFKALAAALVLARVDKGEERLDRRI
                     VVTRADLLEWAPVTSRRVGGAGMTVAELCEAAITVSDNT"
     gene            526..1425
                     /locus_tag="VIN75_14530"
     CDS             526..1425
                     /locus_tag="VIN75_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8975430.1"
                     /translation="MQLPLNAMRAFEASARHLSFTRAALELHVTQAAVSAQVKNLEAR
                     LGTPLFRRLPRGLALTDEGQALLPLLTDSFERMHAVLEQFEGGLHRQVVTVAVVGTFA
                     VGWLLPRLPAFQRAHPAIDLRLMTNNNRVDLAAEGLDWAIRFGDGAWHGTEAQALIAP
                     PLSPVCAPAVARRLRTPADLARETLLRSYRPDEWTRWFAAARVAPPLVRGAIFDSSIT
                     MADAAAQGAGVALLPTALFGEALRSERLVQPFDIGVTLGGYWLTRLKSRPESDAMRTF
                     ADWLQARSGEEDASVRPAGAPAA"
     gene            complement(1397..1864)
                     /locus_tag="VIN75_14535"
     CDS             complement(1397..1864)
                     /locus_tag="VIN75_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975431.1"
                     /translation="MSSGTARAARLVVLAGIVGATCDITYAILYYGWKGISAERILQT
                     VASGLLGKASYDGGWPTAALGLACHYAIVIVAAAVFYAVARRWTWLRSEPVTAGLAFG
                     IAIYVFMNFVVLPLSAYPFPLRFPLVTTATGLLAHMLVGLSIALITRRAHPPA"
     gene            complement(1861..>3409)
                     /locus_tag="VIN75_14540"
     CDS             complement(1861..>3409)
                     /locus_tag="VIN75_14540"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975432.1"
                     /translation="ARHACAQRGDAGHPSLRLMTLEGGRAVEAAMPDRPADPDVVLKV
                     HPGVGWLVAVPQLRFERELTTAERWHEDIARWWRRLQSSAGRASRRDRDPTWQARLQA
                     DEAWWAAQLGIAGETAQALAREARLGGALRPASRIATLPAAQRRQAQVIVIHGGLSSA
                     RSAYEAALNTQARAVAKAIEAGEPASPGGLELRLHLDAQDAATAGARHDLAMAGTAAA
                     FDASALSTAPPPPPDAAHVWPGLPTLDERATWRFEHDTFLRIAQNVAHLVAAVRRQCI
                     AGCPADAQRHIVLVAHSRGGNVARFALPALRKAFGKQGWTFAAVTLGSPHLGTHVFER
                     VGRRWHGLAAAVGGLRHLGAPWLSRDTLAELVNLERGLAYDVPPGFHDVEPAGVERMM
                     KGRPATELPAGMWLVGSHWGPSAGVDVDHRTWDWLFEDMMGAEEESDGLVRRLSALGG
                     RAPENDLVRATPDRCPVQFDASPVFHTHYLVHEQTRRQIARMLGSALGLVSGNNGASC
                     VAQKETA"
BASE COUNT          440 a         1276 c         1246 g          447 t
ORIGIN      
        1 gtgttgtcgc tgacggtgat cgccgcctcg cacagctcgg ccaccgtcat gccggcgccg
       61 ccgacgcgcc tgctcgtcac cggcgcccat tcgagcaggt cggcgcgcgt gacgacgatg
      121 cgccggtcga ggcgttcctc gcccttgtcc acgcgcgcca gcaccagcgc cgcggccagc
      181 gccttgaagg tgctgcacat cgggaagcgc tcgtccaggc gctggcccgc gagctcgccg
      241 gtggcggtgt ccagcatcgc gacgcccagc cggccgccga cctgcgcctc gatcgcgcgc
      301 cagcgctcgg cgcgcgcatc accccagttt ttctgggccg gttcagcgcc gcccagcgcc
      361 cgggcgacgg gccacgcgcc cgccagcgcg atcgccgcgt gggcgaagcg tcttctgtcc
      421 atggatgagt ctcccgctga ttgattcgac aggcggcacg atagccagcg cgagggatcc
      481 catcaaacgc tgatttccgg tgcgagaccc cagataatct ggggcatgca acttcccctg
      541 aacgccatgc gcgccttcga ggcctcggcg cggcacctga gcttcacgcg cgccgcgctg
      601 gagctgcacg tgacgcaggc ggccgtcagc gcgcaggtca agaatctcga ggcccggctc
      661 ggcaccccgc tgttccggcg cctgccgcgc gggctcgcgc tgaccgacga gggccaggcg
      721 ctgctgcccc tgctgaccga cagcttcgag cgcatgcacg cggtgctgga gcagttcgag
      781 ggcggcctgc accggcaggt ggtgaccgtg gccgtcgtcg gcaccttcgc ggtcggctgg
      841 ctgctgccgc ggctgcccgc gttccagcgc gcgcatccgg ccatcgacct gcggctgatg
      901 accaacaaca accgcgtgga cctcgcggca gaggggctgg attgggcgat ccgcttcggc
      961 gacggcgcct ggcacggcac cgaggcgcag gccttgatcg cgccgccgct ctcgccggtc
     1021 tgcgcaccgg ccgtcgcgcg ccgcctgcgc acgcccgccg acctcgcgcg cgagacgctg
     1081 ctgcgctcct accgccccga cgaatggacg cgctggttcg ccgccgcgcg cgtcgcgccg
     1141 ccgctcgtgc gcggcgcgat cttcgactcg tcgatcacga tggccgacgc cgcggcccag
     1201 ggcgcgggcg tcgcgctgct gcccacggcg ctgttcggcg aggcgttgcg cagcgagcgg
     1261 ctggtgcagc cgttcgacat cggcgtgacg ctgggcggct actggctgac gcgcctgaag
     1321 tcgcgccccg agagcgacgc catgcgcacc ttcgccgact ggctgcaggc gcgcagcggc
     1381 gaggaggacg ccagcgtcag gccggcgggt gcgcccgccg cgtgatcagt gcgatcgaca
     1441 ggcccacgag catgtgcgcg agcaggccgg tggccgtggt caccagcggg aagcgcagcg
     1501 ggaacggata ggccgacagc ggcagcacga cgaagttcat gaacacgtag atcgcgatcc
     1561 cgaaggccag gcccgcggtc accggctcgc tgcgcagcca cgtccagcgc ctggccacgg
     1621 cgtagaacac cgccgccgcg acgatcacga tcgcgtagtg gcacgccagc cccagcgccg
     1681 cggtgggcca gccgccgtcg tacgacgcct tgcccagcag gccgctggcg accgtctgca
     1741 ggatgcgctc ggcggagatg cctttccagc cgtagtagag gatcgcgtag gtgatgtcgc
     1801 aggtggcgcc gacgatgccc gcgaggacga ccaggcgcgc cgcgcgtgcg gtgccggaac
     1861 tcatgccgtt tccttttgag ccacgcagga ggcgccattg ttgccggaga ccaggcccag
     1921 cgcgctaccg agcatgcgcg cgatctggcg ccgcgtctgc tcgtgcacca ggtagtgggt
     1981 gtggaacacc ggcgaggcgt cgaactgcac ggggcagcga tcgggcgtgg cgcgcacgag
     2041 gtcgttctcc ggcgcgcgcc cgccgagcgc ggacagccgc cgcaccaggc cgtcgctctc
     2101 ctcctcggcg cccatcatgt cctcgaacag ccagtcccag gtcctgtggt cgacgtcgac
     2161 gccggcgctc ggcccccagt gcgagccgac gagccacatg cccgccggca gctcggtggc
     2221 cggcctgccc ttcatcatcc tttcgacgcc cgcgggctcg acgtcgtgga agccgggcgg
     2281 cacgtcgtag gcgagcccgc gctcgaggtt gacgagttcg gccagcgtgt cgcggctcag
     2341 ccacggcgcg cccagatggc gcagtccgcc gacggccgcg gccaggccgt gccagcgccg
     2401 gcccacgcgc tcgaacacgt gcgtgcccag gtgcgggctg cccagggtga cggcggcgaa
     2461 cgtccagccc tgcttgccga aggccttgcg cagcgcgggc aatgcgaagc gcgcgacgtt
     2521 gccgccgcgc gaatgcgcca ccagcacgat gtggcgctgc gcgtcggcgg ggcagccggc
     2581 aatgcactgg cggcgcacgg cggcgaccag gtgcgccacg ttctgggcga tgcgcaggaa
     2641 ggtgtcgtgc tcgaagcgcc aggtggcgcg ttcgtcgagc gtgggcaggc cgggccagac
     2701 atgcgcggca tcgggcgggg gcggcggcgc cgtcgacagc gccgaggcgt cgaaggccgc
     2761 cgcggtgccg gccatcgcga ggtcatgccg cgcgccggcc gtcgccgcgt cctgcgcgtc
     2821 caggtgcagg cgcagctcga ggccgcccgg tgacgccggt tcaccagcct cgatcgcctt
     2881 ggccaccgcc cgcgcctgcg tgttgagggc cgcctcgtag gcactgcgcg ccgagctcag
     2941 gccgccgtgg atgacgatga cctgcgcctg gcggcgctgc gccgcgggca gcgtggcgat
     3001 gcggctggcc ggacgcagcg cgccgcccag ccgggcctcg cgcgccagcg cctgggccgt
     3061 ttcgccggcg atcccgagct gcgcggccca ccaggcctcg tcggcctgca ggcgcgcctg
     3121 ccaggtcggg tcgcgatcgc gccgggatgc gcggcccgcg ctggactgca ggcgccgcca
     3181 ccatcgcgcg atgtcttcat gccagcgttc ggccgtggtc agctcgcgct cgaagcgcag
     3241 ctgcggcacg gccaccagcc agccgacgcc cggatgcacc ttgagcacga cgtcgggatc
     3301 ggccgggcga tcgggcatcg cggcctcgac cgcgcggccg ccctccagcg tcatcagcct
     3361 gagcgacggg tgccccgcat ccccgcgctg cgcgcaggcg tgccgcgcg
//
LOCUS       DATCRO010000531         4000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_50981, whole genome shotgun sequence.
ACCESSION   DATCRO010000531 DATCRO010000000
VERSION     DATCRO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50981"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2978
                     /locus_tag="VIN75_14545"
     CDS             <1..2978
                     /locus_tag="VIN75_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY8975433.1"
                     /translation="SGPLAAPEAYRVAAGVDGFSMAAGAIPDGARSGRPGWRNAKVTL
                     DANERGGRAHLALDGGSIELPGVFEEPVIGFDRFGADASWKIDPVRGAAQPPAITVAL
                     DDVRFANADAEGSLQLQWQTGPGPAPATPAAGASAPSMFGRGLRYPGTIALKGTLAHA
                     RADRLARYLPKPGLESTRSYIAAAFAGGDLDNVRFNVRGDLAGFPYQRLHDGDFRIDG
                     RVRGTTLTYVPGAPQAGGARAPSPWPAFTQVAGEVVLDRGALEIHDASGRMGELQLHD
                     VNGGVKNLYEQAALGLHGQVSGPMTDFLRYVAQSPVGGWLHNGLASTTATGSAELELG
                     LNIPLQHGEQTTVNGVLTLPGNDVRLVAGAPVLADARARVAFTEKGVTVSGGHARALG
                     GDVAFDGGTLADGALRFTAQGSVSAEGLRKAAEEPVLARLASHVDGQAGYKLVIGIAK
                     GQTEFALTSPLAGLGLALPAPLNKPADATWPLTVSTTASLEEGRPRDTLRVALSQPQG
                     TVLQAEVLRDRSGDAPRPIRAAYAVGAPLPPLQPGGVLMLRGPSFDGDAWLALWRGLA
                     SGPSSAPPPAAGQAAANDDMGISYVPSAAALKTPDFVIGGHHLTNVDVLLAQVVAAGD
                     DVWRSNISSDQTKGVVEYRPETPTRGAQVFARLDRLALDAHDAEPEPAGAASAPAPAA
                     EASQTNSVPALDIVVDNFELNARKLGKLEVAATTQTGGRDWRLTKLAITAPEARLSGS
                     GRWSGAPSRHVSLDFDLELADSGAFLGRLGMGQVLKKGKGKLAGSVTWTGSPLALDYP
                     SLQGRLRVQIDEGQFLKADAGAGRLLGVLSLQSLPRRFMLDFRDLFSEGFAFDNVTGD
                     VVIDRGVASTKDLRMRSATAAVLMSGRADLKAETQDIDVIIVPEVNAGAASLAYAAIN
                     PVIGIGTFLAQLFLRNPLTEAGTREVHVTGTWSAPKVDEVEHKAAVAAAAAAASAARA
                     SEAAAAASAALAQPRTETP"
     gene            3055..3867
                     /locus_tag="VIN75_14550"
     CDS             3055..3867
                     /locus_tag="VIN75_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8975434.1"
                     /translation="MTKFAAVQTVSTPDVSRNLEAAARLVAQAADVGAQWISLPEYFC
                     LMGLRDDAKLHIAEADGDGPIQQFLSDAARRHQVTLVGGTLPILAKKAGHVRNACCVY
                     GPDGKRLARYDKIHLFAFDNGKESYDEARVLEAGDTPVAFEHGGWRAGLSVCYDLRFP
                     ELYRDLMRPPCDVLFVPAAFTHTTGLAHWLLLLRARAVENQCYVVAPAQGGQHENGRR
                     TFGHSVVIDPWGEVLSLALDGAEGEGFAIAELSRERIEQVRMQLPALKHRKL"
BASE COUNT          521 a         1527 c         1452 g          500 t
ORIGIN      
        1 ggtcggggcc gctcgcggcg cccgaggcct accgcgtcgc cgccggcgtc gacgggttct
       61 cgatggccgc cggcgcgatc cccgacggcg cgcgcagcgg ccggcccggc tggcgcaacg
      121 ccaaggtgac gctcgacgcg aacgagcgcg gcggccgcgc gcacctcgcg ctggacggcg
      181 gcagcatcga gctgcccggc gtgttcgagg agccggtgat cgggttcgac cgcttcggcg
      241 ccgacgcgag ctggaagatc gacccggtgc gcggcgccgc gcagccgccg gcgatcacgg
      301 tggcgctcga cgacgttcgc ttcgccaatg ccgatgccga aggcagtctg cagctgcagt
      361 ggcagaccgg cccgggtccc gcgcccgcga cgccggccgc gggcgcgtcc gccccgtcga
      421 tgttcggccg aggcttgcgc tatcccggca cgatcgccct gaagggcacg ctggcgcacg
      481 cgcgcgccga ccgcctggcg cgctacctgc ccaagcccgg gctcgagagc acgcgcagct
      541 acatcgccgc ggcgttcgcg ggcggcgacc tggacaacgt gcgcttcaac gtgcgcggcg
      601 acctcgcggg cttcccttac cagcgcctgc acgacggcga cttccgcatc gacggccggg
      661 tgcgcggcac cacgctcacc tacgtgccgg gcgcgccgca ggccggcggc gcgcgcgcgc
      721 cgtcgccatg gcccgcgttc acgcaggtgg ccggcgaggt ggtgctcgat cgcggcgcgc
      781 tcgagatcca cgacgccagc ggccgcatgg gcgagctgca gctgcacgac gtcaacggcg
      841 gcgtgaagaa tctgtacgag caggcggcgc tggggctgca cggccaggtg tccgggccga
      901 tgaccgactt cctgcgctat gtcgcgcagt cgccggtcgg cggctggctg cacaacgggc
      961 tggcctcgac cacggccacc ggcagcgccg agctcgagct cggcctgaac atcccgctgc
     1021 agcacggcga gcagaccacc gtcaacggcg tgctgaccct gcccggcaac gacgtccggc
     1081 tcgtcgccgg cgcacccgtc ctggcggacg cccgcgcgcg cgtggccttc accgagaagg
     1141 gcgtcaccgt cagcggcggc catgcgcgcg cgctcggcgg cgacgtcgcc ttcgacggcg
     1201 gcacgctggc cgacggcgcg ctgcgcttca cggcgcaggg cagcgtcagc gcggagggcc
     1261 tgcgcaaggc ggccgaggag ccggtgctcg cgcggctggc gtcgcacgtg gacggccagg
     1321 ccggctacaa gctcgtgatc ggcatcgcca aggggcagac cgagttcgcg ctgaccagcc
     1381 cgctcgccgg cctgggcctc gcgctgcccg cgccgctgaa caagcccgcc gacgccacgt
     1441 ggccgctgac ggtgtccacc accgcgtcgc tcgaggaagg ccgtccgcgc gacacgctgc
     1501 gcgtcgcgct gtcgcagccg caggggacgg tgctgcaggc cgaggtgctg cgcgaccgct
     1561 ccggcgacgc gccgcgcccg atccgtgcgg cctacgcggt cggcgcgccg ctgccgccgc
     1621 tgcagccggg cggcgtgctg atgctgcgcg gcccgtcgtt cgatggcgat gcctggcttg
     1681 cgctatggcg cggcctggcc tccggcccgt cctcagcgcc gccgccggcc gccggccagg
     1741 ccgccgccaa cgacgacatg ggcatcagct acgtcccgtc cgccgccgca ctgaagacgc
     1801 ccgacttcgt gatcggcggc caccacctca ccaacgtcga cgtgctgctc gcgcaggtcg
     1861 tcgccgcggg cgacgacgtc tggcgttcca acatctcctc ggaccagacc aagggcgtcg
     1921 tcgagtaccg gcccgagacg cccacgcgcg gcgcgcaggt gttcgcgcgc ctggaccgcc
     1981 ttgcgctcga cgcgcacgac gccgagcccg agccggccgg cgccgcgtcc gccccggcgc
     2041 cggcggccga ggcgtcgcag acgaactcgg tgcccgcgct cgacatcgtc gtcgacaatt
     2101 tcgagctcaa cgccaggaag ctcggcaagc tggaggtggc ggcgacgacg cagacgggcg
     2161 ggcgcgactg gcgcctgacc aagctggcga tcacggcgcc cgaggccagg ctctcgggca
     2221 gcggccgctg gagcggcgcg ccgtcgcgcc acgtctcgct ggacttcgac ctcgagctcg
     2281 ccgattccgg cgcgttcctc gggcgcctgg gcatgggcca ggtgctcaag aagggcaagg
     2341 gcaagctcgc cggctcggtg acatggacgg gctcgccgct ggcgctcgac tacccgagcc
     2401 tgcagggcag gctgcgcgtc cagatcgacg agggccagtt cctcaaggcc gacgccggcg
     2461 ccggccgcct gctgggcgtg ctgagcctgc agtcgctgcc gcgccgcttc atgctcgact
     2521 tccgcgacct gttctcggaa ggtttcgcct tcgacaacgt caccggcgac gtcgtgatcg
     2581 accgcggcgt ggccagcacc aaggacctgc gcatgcgcag cgcgacggcc gcggtgctga
     2641 tgtccggccg cgcggacctg aaggccgaga cgcaggacat cgacgtcatc atcgtgccgg
     2701 aggtcaacgc cggcgcggcc tcgctcgcgt acgccgccat caatccggtc atcggcatcg
     2761 gcaccttcct ggcgcagctg ttcctgcgca acccgctcac ggaggccggc acgcgcgagg
     2821 tgcacgtgac gggcacgtgg tcggcgccca aggtggacga ggtcgagcac aaggccgccg
     2881 tcgccgccgc ggcggccgcg gcgtccgccg cgcgcgccag cgaggccgcg gcggccgcgt
     2941 cggcggcgct ggcccagccg cgtaccgaga cgccctgatc gcgcgcgacg cacccgttca
     3001 cgccggtcgg aaatcgcggg ccggcggata ctcggggcaa cgcaaggaag ccccatgacc
     3061 aagttcgccg ccgtccagac cgtctccacg cccgacgtgt cccgcaacct cgaggccgcc
     3121 gcccgcctgg tcgcccaggc cgccgacgtc ggcgcgcagt ggatctcgct gcccgagtac
     3181 ttctgcctga tgggcctgcg cgacgacgcc aagctgcaca ttgccgaagc ggacggcgac
     3241 gggccgatcc agcagttcct gtccgacgct gcgcgccgcc accaggtcac gctggtgggc
     3301 ggcacgctgc ccatcctcgc gaagaaggcg ggccacgtgc gcaacgcgtg ctgcgtctac
     3361 gggcccgacg gcaagcgcct cgcgcgctac gacaagatcc acctgttcgc gttcgacaac
     3421 ggcaaggagt cctacgacga agcccgcgtc ctcgaggccg gcgacacgcc cgtggccttc
     3481 gagcacggcg gctggcgcgc cggtctgtcg gtctgctacg acctgcgctt tcccgagctg
     3541 taccgcgacc tgatgcgccc gccgtgcgac gtgctgttcg tgcccgccgc cttcacgcac
     3601 accacgggcc tcgcgcactg gctgctgctg ctgcgcgccc gcgccgtcga gaaccagtgc
     3661 tacgtcgtcg cgccggccca gggcggccag cacgagaacg gccgccgcac cttcggccac
     3721 agcgtcgtca tcgatccctg gggcgaggtg ctgtcgctgg cgctggacgg cgccgagggc
     3781 gagggcttcg cgatcgccga gctctcgcgc gagcgcatcg agcaggtgcg catgcagctg
     3841 cccgcgttga agcatcgcaa gctgtgacgg acgcgcgttc gccggccggc ggggccgcgc
     3901 cggcgccgcc gagcctgggc ccggacgcgt tcgtcgcgca ctgcgtcgag tgcatcgcgg
     3961 ccggcggcgg cgccgtggag atcgcgcggc tcatgcggcg
//
LOCUS       DATCRO010000532         4914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_512547, whole genome shotgun sequence.
ACCESSION   DATCRO010000532 DATCRO010000000
VERSION     DATCRO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512547"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VIN75_14555"
     CDS             <1..660
                     /locus_tag="VIN75_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8975435.1"
                     /translation="SPVADVFVVWAKDDEGAIRGFVLEKGWKGLSAPAIHGKVGLRAS
                     ITGEIVMDEVFCPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWHTARQYVL
                     DRKQFGKPLAANQLVQKKLADMQTEITLGLQGCLRLGRMKDEGTAAVEITSIMKRNSC
                     GKSLEIARLARDMLGGNGISDEFGIARHMVNLEVVNTYEGMHDIHALILGRAMTGIAA
                     F"
     gene            819..2015
                     /locus_tag="VIN75_14560"
     CDS             819..2015
                     /locus_tag="VIN75_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8975436.1"
                     /translation="MTATPRSLAGVKVLDLSRVLAGPWCGQLLADLGADVVKVERPGA
                     GDDTRSWGPPWLKDANGEQTRDAAYYFCANRNKRSVTIDMATPEGQALVKRLAMEADV
                     VLENFKVGGLTQYGLDYASLKALKPSLVYCSITGFGQTGPYAQRAGYDFLIQGMGGLM
                     SVTGRAEGEEGAGPQKVGVALTDILTGLYATVGVLAALAHRDRTGEGQHIDLALLDVQ
                     VACLANQTANYLIGGAVPRRMGNAHPNIVPYQDFPTADGDMILAVGNDGQFAKFCEVA
                     GKSEWARDERFATNAARVKHRAELVVLLRQTTVMRTTHDWIVALEAAGVPCGPINRLD
                     EVFADPQVVARGLRIDLPHAVAGTVPGVANPIRLSASPVAYDRGPPVLGEHTADVLRE
                     WLGMGA"
     gene            complement(2219..2437)
                     /locus_tag="VIN75_14565"
     CDS             complement(2219..2437)
                     /locus_tag="VIN75_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY8975437.1"
                     /translation="MTASAIPPGLEGPERDIQCPGCGKPCKYGPSNPSRPFCSDRCRN
                     GDFGAWATESYRVPAAQNPVDEQDSEGE"
     gene            complement(2466..3221)
                     /gene="zapD"
                     /locus_tag="VIN75_14570"
     CDS             complement(2466..3221)
                     /gene="zapD"
                     /locus_tag="VIN75_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY8975438.1"
                     /translation="MVLYEYPFNEGIRTMLRLEHLFDRMAGLIARDEPVDHHYALASM
                     FEIMDVAARADLKSDVLKELDRHKQQLLSYRGNPAISEAALDDVIGRIDVAFTSLNES
                     PGKAGQALTSNEWLMSIRSRIGIPGGTCAFDLPAYYAWQQHAPEQRRADLQRWTDTLT
                     PLSTSLHLLLGLLRESGVPHKVVAIGGQYQQSLTAARTWHLLRVKLPQEADLVPEISG
                     HRLMVSIRLMKSDSEGRLRPLAEDCAFELALCS"
     gene            complement(3326..3952)
                     /gene="coaE"
                     /locus_tag="VIN75_14575"
     CDS             complement(3326..3952)
                     /gene="coaE"
                     /locus_tag="VIN75_14575"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430449.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8975439.1"
                     /translation="MGGHPLPGLRIGLSGGIGSGKSSVARRLAALGALVVDTDALAHA
                     LTAPGGGAIPAIAAAFGDDMLAADGSMDRAKMRALVFGDPAQRRRLEAILHPMIAAGT
                     RAQASRAGAGQAVVFDVPLLTESGAWRARVDRVLVVDCPTATQVARVVARSGWTPEAV
                     ERTIAQQATRAQRRAIADAVIVNDTLTLAQLDGEVDALCAAWNLGGTA"
     gene            complement(3925..4869)
                     /locus_tag="VIN75_14580"
     CDS             complement(3925..4869)
                     /locus_tag="VIN75_14580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8975440.1"
                     /translation="MDEFLTDLQWVCGSPVVLGVFGLLVGSFLNVVIHRKPIIMMREW
                     LLDFGGMLGDGEVWKQVFGKPQPAEITEAGRSIDAHLEALTPLGLATPRSRCGACGHE
                     IRWYENIPLLSWLALRAKCSACGTRISVRYPLVELGTGLLFGLAGWKFGPHLATLGWC
                     AGIALLVAMALIDLDTKYLPDDLTYPLLWLGLLMAALGGTIPLSTAVWGAIAGYVPLW
                     LIATGFALLMKRQGMGGGDLKLLAALGAWLGWQSILPIILIASLVGLCVNVPRMLLGK
                     HQRHQQYPFGPFLAGGGLFVMFVGNDTLLSWAGIRFPA"
BASE COUNT          762 a         1685 c         1763 g          704 t
ORIGIN      
        1 tcgccggtcg ccgacgtgtt cgtggtctgg gccaaggacg acgagggcgc gatccgcggc
       61 ttcgtgctgg agaagggctg gaagggcctg tcggcgccgg cgatccacgg caaggtgggc
      121 ctgcgcgcgt cgatcaccgg cgagatcgtg atggacgagg tgttctgccc ggaagagaac
      181 gccttccccg aggtgcgcgg cctcaagggc ccgttcacct gcctgaactc ggcgcgctac
      241 ggcatcgcct ggggcgcgct cggcgccgcc gaggactgct ggcacaccgc gcgccagtac
      301 gtgctcgatc gcaagcagtt cggcaagccg ctcgcggcca accagctggt gcagaagaag
      361 ctggccgaca tgcagaccga gatcacgctg ggcctgcagg gctgcctgcg cctgggccgc
      421 atgaaggacg agggcacggc cgcggtggag atcacgtcga tcatgaagcg caattcgtgc
      481 ggcaagtcgc tcgagatcgc gcgcctggcc cgcgacatgc tgggcggcaa cggcatctcc
      541 gacgagttcg gcatcgcccg ccacatggtc aacctcgaag tggtcaacac ctacgaaggc
      601 atgcacgaca tccatgcgct gatcctgggc cgtgcgatga ccggcatcgc tgccttttaa
      661 cccccacggt cgctggcgct ccctgccccc cgagggggcc ggccgcgagc ggcccggcag
      721 agccggctcc gccgctccct cgaatttccg ttgaggtgcg cgagcggcag cgccggggca
      781 ggcccggtgc cagcagcttc tcttcgatcg gagtcttgat gaccgcaacc cctcgctcgc
      841 tcgcgggcgt gaaagtcctg gacctctcgc gtgtgctcgc ggggccgtgg tgcggccagc
      901 tgctggctga tctcggcgcg gacgtcgtga aggtcgagcg cccgggcgcc ggcgacgaca
      961 cgcgctcgtg gggcccgccg tggctcaagg acgcgaacgg cgagcagacg cgcgacgccg
     1021 cgtactactt ctgcgccaac cgcaacaagc gctcggtgac gatcgacatg gccacgccgg
     1081 agggccaggc gctggtcaag cggctggcga tggaagccga cgtcgtgctc gagaacttca
     1141 aggtcggcgg cctgacgcag tacggcctcg actacgcgag cctcaaggcg ctgaagccgt
     1201 cgctggtcta ctgctcgatc accggcttcg gccagacggg cccgtacgcg cagcgcgccg
     1261 gctacgactt cctgatccag ggcatgggcg gcctcatgag cgtcaccggc cgcgccgagg
     1321 gcgaggaggg cgcggggccg cagaaggtcg gcgtcgcgct gaccgacatc ctcacgggcc
     1381 tgtacgccac cgtcggcgtg ctggccgcgc tcgcgcaccg cgaccgcacc ggcgagggcc
     1441 agcacatcga cctcgcgctg ctggacgtgc aggtcgcgtg cctggccaac cagacggcca
     1501 actacctgat cggcggcgcc gtgccgcgcc gcatgggcaa cgcgcacccg aacatcgtgc
     1561 cctaccagga cttcccgacg gccgacggcg acatgattct ggccgtgggc aacgacggcc
     1621 agttcgcgaa gttctgcgag gtcgcgggca agtccgagtg ggcgcgcgac gagcgcttcg
     1681 ccaccaacgc ggcacgcgtg aagcatcgcg ccgagctggt cgtgctgctg cgccagacca
     1741 ccgtcatgcg cacgacgcac gactggatcg tcgcgctgga agcggccggc gtgccctgcg
     1801 gcccgatcaa ccggctcgac gaggtgttcg ccgatccgca agtggtcgcc cgcggcctgc
     1861 gcatcgacct gccgcatgcc gtggccggca cggtgccggg cgtggccaac ccgatccgcc
     1921 tgtcggcctc gccggtggcc tacgaccgcg ggccgccggt gctgggcgag catacggccg
     1981 acgtgctgcg cgagtggctg ggcatggggg cctgaagcga ggggtctggc ctctcctcat
     2041 gtcgccgtcg cgcggtgacc tgcagagaag atgttgccgg cgacttgagg cgaaggcctg
     2101 acctcgatcg tcagccagtg atcggggtca ggccctcgcc tcgcatcgcg gccgaggtgt
     2161 cgactcgcgg cagctttgcg gcgcgatgtc gaggagaggc cagacccctc tgccgtgctc
     2221 actcgccctc ggaatcctgc tcgtcgaccg ggttctgcgc cgccggcacg cggtacgact
     2281 cggtcgccca cgcgccgaaa tcgccgttgc ggcagcggtc gctgcagaat gggcgtgacg
     2341 ggttggacgg gccatacttg cagggcttgc cgcagcccgg gcactggatg tcgcgctcgg
     2401 ggccttcgag gcccggcggg atggcggaag cggtcatgca ggaatactcg tgaggccggc
     2461 gtgcgtcagg agcacagcgc cagctcgaag gcgcagtcct cggcgagcgg gcgcaggcgc
     2521 ccttcgctgt cggacttcat caggcgaatc gacaccatca accggtggcc gctgatctcg
     2581 gggacgagat cggcctcctg cggcagcttc acgcgcagca ggtgccaggt gcgcgccgcg
     2641 gtcaggctct gctggtactg gccgccgatg gccaccacct tgtgcggcac gccggactcg
     2701 cgcagcagcc ccagcagcag gtgcagcgac gtcgacaggg gcgtgagcgt gtccgtccag
     2761 cgctgcaggt cggcgcgccg ctgctccggc gcgtgctgct gccaggcgta ataggccggc
     2821 agatcgaacg cgcaggtgcc gccggggatg ccgatgcggc tgcggatgct catcagccac
     2881 tcgttggacg tgagcgcctg gccggccttg ccgggggatt cgttgaggct cgtgaaggcg
     2941 acgtcgatgc ggccgatgac gtcgtcgagc gcagcctccg agatggccgg attgccgcga
     3001 tagctcagca actgctgctt gtggcgatcc agttccttga gcacgtcgga cttgaggtcg
     3061 gcacgcgccg cgacgtccat gatctcgaac atcgaggcca gcgcgtagtg gtggtcgacc
     3121 ggttcgtcgc gcgcgatgag gccggccatg cggtcgaaca ggtgttcgag ccggagcatc
     3181 gtgcggatgc cctcgttgaa cggatattcg tataggacca aggcgttctc tggttcggcg
     3241 gtgagcgcga ttgtttcaca cgcgttacga gcttcgctcc cctaaacgcg caagtgaccg
     3301 caatctgcag ctttgtccta caagatcacg ccgtgccgcc gaggttccat gccgcgcaca
     3361 acgcgtcgac ctcgccgtcg agctgcgcga gcgtgagcgt gtcgttgacg atgaccgcgt
     3421 cggcgatcgc gcggcgctgc gcgcgcgtgg cctgctgggc gatcgtgcgc tccacggcct
     3481 cgggcgtcca gcccgaacgc gcgacgaccc gtgcgacctg cgtcgccgtc gggcagtcga
     3541 ccaccagcac gcggtcgacg cgtgcgcgcc aggcgcccga ctcggtgagc agcggcacgt
     3601 cgaacaccac ggcctggccc gcgcccgcgc gtgacgcctg cgcgcgcgtg cccgcggcga
     3661 tcatggggtg caggatggcc tcgagccgcc ggcgctgcgc cgggtcgccg aagacgaggg
     3721 cgcgcatctt cgcgcgatcc atggagccgt cggccgcgag catgtcgtcg ccgaacgcgg
     3781 cggcgatggc ggggatggcg ccaccgccgg gagcggtcaa cgcgtgggcg agggcgtcgg
     3841 tgtcgaccac cagcgcgccg agcgccgcga ggcgccgcgc gacactgctc ttgccactgc
     3901 cgatgccgcc gctgaggccg atcctcaggc cgggaagcgg atgcccgccc atgacagcag
     3961 ggtgtcgttg ccgacgaaca tcacgaacag gccgccgccg gccaggaacg ggccgaacgg
     4021 gtattgctgg tggcgctggt gcttgcccag cagcatgcgc ggcacgttga cgcacaggcc
     4081 cacgagggag gcgatcagga tgatgggcag gatcgactgc cagcccagcc acgcgccgag
     4141 cgcggcgagc agcttgaggt cgccgccgcc catgccctgg cgcttcatca gcagcgcgaa
     4201 gccggtggcg atcagccaca gcggcacgta gccggcgatg gcgccccaca ccgcggtcga
     4261 cagcgggatc gtgccgccga gcgcggccat cagcagcccg agccacagca gcgggtaggt
     4321 gaggtcgtcg ggcaggtact tggtgtccag gtcgatcagc gccatcgcca ccagcagcgc
     4381 gatgccggcg caccagccca gcgtggccag gtgcggcccg aacttccagc ccgcgagccc
     4441 gaacagcaac ccggtgccga gctcgacgag cggatagcgc accgagatgc gcgtgccgca
     4501 cgccgagcac ttggcgcgca gcgcgagcca gctcaggagg gggatgttct cgtaccagcg
     4561 gatctcgtgc ccgcaggcgc cgcagcgcga gcgcggcgtg gccaggccca gcggtgtcag
     4621 cgcctcgaga tgggcgtcga tgctgcggcc ggcctcggtg atctcagccg gctgcggctt
     4681 gccgaagacc tgcttccaga cctcgccgtc gcccagcatg ccgccgaagt cgagcaacca
     4741 ctcgcgcatc atgatgatcg gcttgcgatg gatcaccacg ttgaggaagc tgccgaccag
     4801 gagtccgaag actcccagca cgacgggcga accgcagacc cactgcaggt cggtcaggaa
     4861 ctcgtccaac gctcagacca cttggccgat cttgaagatg ggcaggtaca tcga
//
LOCUS       DATCRO010000533         7492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_748595, whole genome shotgun sequence.
ACCESSION   DATCRO010000533 DATCRO010000000
VERSION     DATCRO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748595"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1077
                     /locus_tag="VIN75_14585"
     CDS             262..1077
                     /locus_tag="VIN75_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication/partition related protein"
                     /protein_id="HEY8975441.1"
                     /translation="MNIVVREDLKSYIEPLTPDEHDALERSLLAEGCRDALVLWGDVL
                     VDGHNRYGLCRKHGLPFNTVQNPRFQSIEDVHLWMIDQHLGRRSVSDYQRGVLALRKK
                     EIVAARESAAKAAKKDAHMRAEVLGQAEPLGSPIDGEPVAAAEPPIKSREDIAKAARL
                     SPTQVTMIEKIQKQAAPEVVAAVRAGSISLNAAAAVASLPVEEQVAAASAGKDELKQA
                     AKRAREATRKPRVEASQMPTTEVQALQDRIAELTAENEYLRGQVAELQARLAG"
     gene            1400..1762
                     /gene="clpS"
                     /locus_tag="VIN75_14590"
     CDS             1400..1762
                     /gene="clpS"
                     /locus_tag="VIN75_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY8975442.1"
                     /translation="MPEENPPPPPPPALPKPVLPDSGQGAVVAERKAARTKPPRMYQV
                     VLLNDDFTPMEFVVMVLQEYFKLDLETSTQIMLKIHHEGRGVCGLFTKDIAATKVELV
                     LAAARRYGHPLQCIMEAA"
     gene            1759..4068
                     /gene="clpA"
                     /locus_tag="VIN75_14595"
     CDS             1759..4068
                     /gene="clpA"
                     /locus_tag="VIN75_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY8975443.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIEDLRKSLSTFIRENTPTVGGSEEVDTQPTLGFQRVIQRAIMHVQSTGGGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNYIAHGIKKSDPPEPPKASGESAG
                     EQAEKDEGGEGKGSPLDQFTQNLNQMARDGKIDPLIGREHEVERVIQVLCRRRKNNPL
                     LVGEAGVGKTAIAEGLAWRITQNDVPEVLADAVVYSLDMGALLAGTKYRGDFEQRLKG
                     VLKQLKDQPRAVLFIDEIHTLIGAGAASGGTLDASNLLKPALSNGTMKCIGATTFTEY
                     RGIFEKDAALSRRFQKVDVNEPTVEQTVEILKGLKSRFEEHHSVKYAIGALQAAAELS
                     AKYINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTITRNEVEEIVAKIARIPPASVTT
                     DDRDKLRTIDRDLKSVVFGQDPAIDALSAAIKMARSGLGKPDKPIGSFLFSGPTGVGK
                     TEVAKQLAFILGIDLIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEQITKKPHC
                     VLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKSDFRNVIIIMTTNAGAETMSKATI
                     GFTNSRQQGDEMGDIKRMFTPEFRNRLDAIVNFKAIDEEIILRVVDKFLLQLEAQLAE
                     KKVEVTFSDALRKHLGKKGFDPLMGARPMQRLIQDTIRRALADELLFGRLVDGGRLGV
                     DVDDKGETVLDIQPSGKSGDRPKAEAAAL"
     gene            complement(4361..4654)
                     /locus_tag="VIN75_14600"
     CDS             complement(4361..4654)
                     /locus_tag="VIN75_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975444.1"
                     /translation="MNFKAIALMGALCTGLAACGGDDTIPQTRTLSAGQSYTIGPNQT
                     FYVPSNTTVDSTGSHVTITGHKNTITAAVGAVVTVAADATGPADNIVVSHGAN"
     gene            complement(4764..6053)
                     /locus_tag="VIN75_14605"
     CDS             complement(4764..6053)
                     /locus_tag="VIN75_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY8975445.1"
                     /translation="MKTPFWRILYVQVLFAIVVGALLGHFDPGLATKMKPLGDGFVAL
                     IRMVIAPIIFCTVVAGIAGMDDMKKVGRVGGKALLYFEVMSTIALLVGLMVAHLLRPG
                     DGFNVDPKTLDASAVSSYATQAHGQSTVDFLLKIIPTTVVDAFAKGEVLQVLLVALLF
                     GAALAHAGERGAPLLHLIDRFAAVFFRMVTLITRLAPLGAFGAMAFTVGKYGLGSLLP
                     LLKLVATFYVTSLLFVLVVLGLVARVTGFSILRFLVYIKEELLIVLGTSSSEAALPQL
                     MAKLENLGCSKSVVGLVVPTGYSFNLDGTNIYMTLAVIFIAQALDVDLTVGQEATILA
                     VAMLTSKGASGVTGSGFITLAATLSVVPSLPVTGMVLILGIDKFMSECRALTNICGNG
                     VACVVVSAWERELDRGRLAEALRRDTPGETEAAEPAA"
     gene            complement(6145..6948)
                     /locus_tag="VIN75_14610"
     CDS             complement(6145..6948)
                     /locus_tag="VIN75_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY8975446.1"
                     /translation="MNDAAPPAWWRAPLARLGAWREQRALARRAIPDDLWALTLHRLP
                     FLARLDEAERAELRRLCSLFLDRKQFHGAGGLEVTDDMALMIAAQACLPVLKLGLHFY
                     DPVLGIIVHPDQMVVQESIMDEDGVVHEFEDERAGELTNRGLIELVWPEVESAGEIPD
                     EAYNVVIHEFAHLIDAATGDIDGVPPLPDRAARERWIAVIDPAYDAFCRLVDRGVETV
                     VDPYGAEAVDEFFAVAVEAFFVTPAPFKRESPALYDLLAGYFLQDPAAA"
     gene            complement(6953..>7492)
                     /locus_tag="VIN75_14615"
     CDS             complement(6953..>7492)
                     /locus_tag="VIN75_14615"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8975447.1"
                     /translation="ATAARVPVWLQHGNRDFLIGAEFAQATGIRLLADPASVTAFGRT
                     VLLTHGDELCVDDVPYQVFRQQVRNPAYQQQLLSQPLEARLALAAKMRAASLARHDEP
                     VTYADVDETMAKAWLARLGTDTMIHGHTHRPGTQVRDGWTRHVLTDWDLDKPLPEARR
                     AQVLRWTAAGFDRISPEQA"
BASE COUNT         1298 a         2557 c         2573 g         1064 t
ORIGIN      
        1 ggtctgcctc actcatgcgt ttcgagtttt cgacaaatga cttgaggttc gtgaggtaag
       61 gcattgaaat aatgggattc tggcgccgcc gcgtcacgat gccgcaagct cgaccgtggt
      121 cgccgccgcc gtgctcggaa cgccaggcgc gcgccgcccg gggcggggcg gcgacgccct
      181 tagaatgacg cccatcccct gtcgaaggcc cgccgcgcgc gggccttcgc gcctcccggc
      241 ccctgacatc cccatcgccc cgtgaacatc gtcgtccgag aagacctcaa gagctacatc
      301 gagccgctga cccccgacga gcacgacgcg ctcgagcgca gcctgctggc cgaaggctgc
      361 cgcgacgcgc tggtgctgtg gggcgacgtg ctggtcgacg gccacaaccg ctacggcctg
      421 tgccgcaagc acgggctgcc gttcaacacg gtgcagaacc cgcgcttcca gtcgatcgag
      481 gacgtccacc tgtggatgat cgaccagcac ctgggccgcc gcagcgtctc ggactaccag
      541 cgcggcgtgc tcgcgctgcg caagaaggag atcgtggccg cgcgcgagtc ggcggccaag
      601 gccgcgaaga aggatgcgca catgcgcgcc gaggtgctgg gccaggccga gccgctcggc
      661 tcgccgatcg acggcgagcc ggtggccgcc gccgagccgc cgatcaagtc gcgcgaggac
      721 atcgccaagg ccgcgcgcct gagcccgacg caggtgacga tgatcgagaa gatccagaag
      781 caggcagcgc ccgaggtggt ggccgcggtg cgggcgggca gcatctcgct caacgccgcg
      841 gcagccgtcg ccagcctgcc ggtggaggag caggtcgcag cggcctcggc cggcaaggac
      901 gagctcaagc aggccgccaa gcgtgcgcgc gaggcgacca ggaagccgcg cgtcgaggcc
      961 agccagatgc cgaccaccga ggtgcaggcg ctgcaggatc gcatcgccga gctgacggcg
     1021 gagaacgagt acctgcgcgg ccaggtggcc gaactgcagg ccaggctcgc gggctgaacg
     1081 ccccgccgcg ggtcatgccg tcgcgggagc ggccggcagc aggcaaattc ctcgtcggct
     1141 cggggcaaca gggccccaac gcgggcgcgc gacgtgctac cttggggtca tgagaaagcg
     1201 cttttccagc cgccgccggg cgttcgtcca gcccctggcg gccggcggga aagttgtcca
     1261 gtacgctcgt cttgaaatga ttcgcgtcgt cccgaaatgc gtggcctggg caaagcttat
     1321 ccggcaatgc tctttcccct gtcagggcga cgatatgtcc catcgtgccc ggacgtgcgc
     1381 gagtctatag aatcaattca tgcccgagga aaaccctcca ccgccgccac cgcccgcact
     1441 gcccaagccg gtcctgccgg actcgggcca gggcgcggtc gtggccgaac gcaaggctgc
     1501 ccgaaccaag ccgccccgca tgtaccaggt ggtgctgctc aatgacgact tcaccccgat
     1561 ggagttcgtc gtcatggtgc tgcaggaata tttcaagttg gacctcgaaa cctccacgca
     1621 aatcatgctg aagatccatc atgaggggcg tggcgtgtgc ggcctgttca ccaaggacat
     1681 cgccgccacg aaggtcgagc tggtactggc cgccgcacgt cggtacggac acccgcttca
     1741 atgcattatg gaggccgcat gattgcgcaa gaactggaag tcagcctgca catggcgttt
     1801 gtcgaggcgc gtcagcagcg gcacgagttc atcaccgtcg agcacctgct gctcgcgctg
     1861 ctggacaacc cctcggcggc cgaggtcctg cgtgcctgcg cagccaacat cgaggacctg
     1921 aggaagagcc tgtccacctt catccgcgag aacacgccca ccgtaggcgg ctccgaagag
     1981 gtcgacaccc agccgacgct gggcttccag cgcgtgatcc aacgcgcgat catgcacgtg
     2041 cagtccaccg gcggcggcaa gaaggaagtc accggggcca acgtgctcgt ggcgatcttc
     2101 ggcgagaagg attcgcatgc cgtgtactac ctgcaccagc agggcgtcac gcgcctggac
     2161 gtcgtcaact acatcgccca cggcatcaag aagtcggatc cgccggagcc gcccaaggcc
     2221 agcggcgagt ccgccggcga gcaggccgag aaggacgagg gcggcgaggg caagggttcc
     2281 ccgctggacc agttcaccca gaacctcaac cagatggcac gcgacggcaa gatcgatccg
     2341 ctgatcggcc gcgagcacga ggtcgagcgc gtcatccagg tgctgtgccg ccgccgcaag
     2401 aacaacccgc tgctggtcgg cgaggccggc gtgggcaaga cggccatcgc cgagggcctg
     2461 gcatggcgca tcacgcagaa cgacgtgccc gaagtcctcg cggacgcggt cgtctactcg
     2521 ctggacatgg gcgcgctgct ggcgggcacc aagtaccgcg gcgacttcga gcagcgcctc
     2581 aagggcgtgc tcaagcagct caaggaccag ccgcgcgcgg tgctgttcat cgacgagatc
     2641 cacacgctga tcggcgccgg cgcggcgtcg ggcggcacgc tggacgcgag caacctgctc
     2701 aagccggcgc tgtccaacgg cacgatgaag tgcatcggcg ccaccacgtt caccgagtac
     2761 cgcggcatct tcgagaagga cgcggcgctg tcgcggcgct tccagaaggt cgacgtcaac
     2821 gagccgaccg tggagcagac cgtcgagatc ctcaagggcc tgaagtcgcg cttcgaggag
     2881 caccacagcg tcaagtacgc catcggcgcg ctgcaggccg cggcggagct gtcggccaag
     2941 tacatcaatg accgtcacct gcccgacaag gcgatcgacg tcatcgacga ggccggcgcg
     3001 gcacagcgca tcctgcccaa gagcaagcag aagaagacga tcacccgcaa cgaggtggaa
     3061 gagatcgtgg ccaagatcgc gcgcatcccg cccgcctcgg tcaccaccga cgaccgcgac
     3121 aagctgcgca cgatcgaccg cgacctcaag agcgtggtgt tcggccagga tccggccatc
     3181 gacgcgctgt cggcggccat caagatggcg cgctccggcc tgggcaagcc cgacaagccg
     3241 atcggctcgt tcctgttctc cgggcccacg ggtgtcggca agaccgaggt cgccaagcag
     3301 ctcgcgttca tcctgggcat cgacctgatc cgcttcgaca tgtccgagta catggagcgc
     3361 cacgccgtca gccggctgat cggcgcgcct ccgggctacg tcggcttcga ccagggcggc
     3421 ctgctgaccg agcagatcac caagaagccg cactgcgtcc tgctgctcga cgagatcgag
     3481 aaggcgcacc cggacgtctt caacgtgctg ctgcaggtga tggaccacgg cacgctgacc
     3541 gacaacaacg gccgcaagtc cgacttccgc aacgtgatca tcatcatgac caccaacgcg
     3601 ggcgccgaga ccatgagcaa ggcgacgatc ggcttcacca actcgcgcca gcagggcgac
     3661 gagatgggcg acatcaagcg catgttcacg ccggagttcc gcaaccgcct ggacgcgatc
     3721 gtcaacttca aggccatcga cgaggagatc atcctgcgcg tggtcgacaa gttcctgctg
     3781 cagctcgagg cccagctggc cgagaagaag gtcgaggtca cgttctccga cgccctgcgc
     3841 aagcacctgg gcaagaaggg cttcgatccg ctgatgggcg cgcgcccgat gcagcgcctg
     3901 atccaggaca cgatccgtcg cgcgctggcc gacgagctgc tgttcggccg cctggtcgac
     3961 ggcggtcgcc tgggcgtgga cgtcgacgac aagggcgaga cggtgctcga catccagccc
     4021 tcgggcaaga gcggcgacag gcccaaggcg gaagccgcgg cgctctgatc ggcggccttc
     4081 ccacgaagcc cggcgcgacg caagtcccgc cgggcttttt cacggctctt cgtcgatttg
     4141 cggggattcg gtcgacgcga gaccctgctg tgacgaagaa caaacgtgtc atcctgcgcg
     4201 cagtcgcacg atccgcgccg gccgagcgac ggtctgggcc atcctcgtgg tgcccagatg
     4261 gatcctgcga cttcgcgcag gatgacatgg tgggtgcagg cggcgcttgc ttcgacacgg
     4321 ccacgcaccc cgtgagccgg cgaagagcct ctttcacggg ctagtttgcc ccgtgcgaca
     4381 ccacgatgtt gtcggccggc cccgtcgcgt cggccgcgac ggtgacgacc gccccgaccg
     4441 cggccgtgat cgtgttcttg tggcccgtga tcgtgacgtg gctgccggtc gagtcgacgg
     4501 tcgtgttcga cggcacgtag aacgtctggt tgggcccgat ggtgtacgac tggccggcgc
     4561 tgagggtgcg cgtctgggga atggtgtcgt ccccgccgca cgcggccagg cccgtgcaga
     4621 gcgcgcccat gagcgcgatc gccttgaagt tcatgcagtt cctcccgggt gtcgtctttt
     4681 gaagaggcgc aagcgtaggc cgcctgcctg acggcggcct gtgcccgcgg atcaggcttg
     4741 cccccttgac tatagcgacg cgttcatgcg gccggctcgg cggcctcggt ctcgccgggc
     4801 gtgtcgcggc gcagcgcctc ggccagccgg ccccgatcga gctcgcgctc ccaggcggag
     4861 acgacgacgc aggccacgcc gttgccgcag atgttggtca gcgcgcggca ctcgctcatg
     4921 aacttgtcga tgcccaggat cagcaccatg ccggtcacag ggagcgatgg gacgaccgac
     4981 agcgtggccg ccagcgtgat gaaacccgag ccggtgacgc cgctcgcgcc cttggacgtg
     5041 agcatcgcga ccgccaggat cgtcgcttcc tggccgacgg tcaggtcgac gtcgagcgcc
     5101 tgcgcgatga agatcaccgc cagggtcatg tagatgttgg tgccgtcgag gttgaacgag
     5161 tacccggtgg gcaccaccag gccgacgacc gacttcgagc agccgaggtt ctcgagcttg
     5221 gccatcagct gcggcagcgc ggcctccgag gaactggtgc ccagcacgat cagcaactcc
     5281 tccttgatgt agacgaggaa gcgcaggatc gagaagcccg tgacgcgtgc gaccagcccg
     5341 aggacgacca gcacgaacag cagcgacgtg acgtagaagg tggccaccag cttgagcagc
     5401 ggcagcaggg agcccagccc gtacttgccc acggtgaagg ccatcgcgcc gaatgcgccc
     5461 aggggcgcga ggcgcgtgat cagggtgacc atgcggaaga agacggccgc gaagcggtcg
     5521 atcaggtgca gcagcggcgc cccgcgctcg cccgcgtgcg cgagggccgc gccgaacagc
     5581 agcgccacca gcagcacctg cagcacctcg cccttggcga acgcgtcgac gaccgtggtc
     5641 gggatgatct tgagcaggaa gtcgacggtg ctctggccgt gcgcctgcgt ggcgtaggac
     5701 gacaccgcgc tcgcgtccag cgtcttcggg tccacgttga agccgtcgcc gggccgcagc
     5761 aggtgcgcca ccatcaggcc gaccagcagc gcgatggtcg acatcacctc gaagtagagc
     5821 agggccttgc cgccgacccg ccccaccttc ttcatgtcgt ccatgcccgc gatgccggcg
     5881 acgacggtgc agaagatgat gggcgcgatc accatgcgga tcagcgcgac gaagccgtcg
     5941 cccagcggct tcatcttcgt cgccagcccg ggatcgaagt ggccgagcag ggcgcccacg
     6001 acgatcgcga acaggacctg gacgtacagg atcctccaga acggcgtctt catgtcgcgc
     6061 actcccggta cggcgccggg cggcgtcgga acgacgattc ttggccgcac acgatcccca
     6121 tccctgcggg agggggaagc cggttcaggc ggccgccggg tcctgcagga agtagccggc
     6181 cagcaggtcg tagagcgccg gcgattcgcg cttgaagggc gccggggtca cgaagaaggc
     6241 ctcgaccgcc accgcgaaga actcgtcgac cgcttcggcg ccgtagggat cgaccaccgt
     6301 ctccacgccc cggtccacca ggcggcagaa ggcgtcatag gcggggtcga tgaccgcgat
     6361 ccagcgctcg cgggcggcgc gatcgggcag cggcgggacg ccgtcgatgt cgcccgtcgc
     6421 ggcgtcgatc aggtgcgcga actcgtggat gacgacgttg taggcctcgt ccgggatctc
     6481 gcccgcggac tcgacctccg gccagaccag ctcgatcagg ccgcggttgg tgagctcgcc
     6541 ggcgcgctcg tcctcgaact cgtggacgac gccgtcctcg tccatgatgc tttcctgcac
     6601 gaccatctgg tcggggtgca cgatgatgcc cagcaccggg tcgtagaagt gcagccccaa
     6661 tttcagcacc ggcaggcagg cctgcgccgc gatcatcagc gccatgtcat cggtgacctc
     6721 gaggccgccc gcgccgtgga actgcttgcg atcgaggaac aggctgcaca gccgccgcag
     6781 ctcggcgcgc tcggcctcgt ccaggcgggc gaggaagggc agccggtgca gcgtcagcgc
     6841 ccacaggtcg tcggggatcg cgcgccgcgc gagcgcgcgc tgctcgcgcc acgcgcccag
     6901 gcgcgcgagc ggcgcgcgcc accacgcggg cggggcagcg tcgttcatcc ggtcacgcct
     6961 gttcgggcga gatgcggtcg aagccggcgg ccgtccagcg cagcacctgc gcgcggcgcg
     7021 cctcgggcag gggcttgtcc agatcccagt cggtcaggac gtggcgcgtc cagccgtcgc
     7081 gcacctgcgt gccggggcga tgggtgtggc cgtggatcat ggtgtcggtg cccaggcgcg
     7141 cgagccaggc cttggccatc gtctcgtcga cgtcggcgta ggtgacgggc tcgtcgtggc
     7201 gcgccaagct ggcggcgcgc attttcgcgg cgagtgccag ccgagcctcc agcggctgcg
     7261 acagcagctg ctgctggtac gccgggttgc gcacctgctg gcggaacacc tggtacggca
     7321 cgtcgtcgac gcacagctcg tcgccgtgcg tgagcagcac cgtgcggccg aacgccgtca
     7381 ccgaggccgg atcggccagc aggcggatgc ccgtggcctg cgcgaactcg gcgccgatca
     7441 ggaagtcgcg gttgccgtgc tgcagccaca ccggcacgcg cgcggcggtg gc
//
LOCUS       DATCRO010000534         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_242052, whole genome shotgun sequence.
ACCESSION   DATCRO010000534 DATCRO010000000
VERSION     DATCRO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242052"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VIN75_14620"
     CDS             <1..160
                     /locus_tag="VIN75_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEY8975448.1"
                     /translation="KGDAIKTGQPLAVLEAMKMEHTIAAPRDGTVAELLFAPGDQVSE
                     GGELLTLE"
     gene            164..1093
                     /locus_tag="VIN75_14625"
     CDS             164..1093
                     /locus_tag="VIN75_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8975449.1"
                     /translation="MPQFPEFVKIVDVGPRDGLQNEKNHVPAADKIALVSLLQDAGLR
                     EIEVTSFVSPKWVPQMADALDVMAGIARKPGVRYSVLTPNMKGWEAAAATRPDEIVVF
                     GAASEAFSQKNINCSIAESIDRFAPVVAAAKAAGVKVRAAISCAVGCPYEGEVAPSRV
                     GMVARMMRAIGVDHVGVADTIGVGTPRKVRAAMEAALEHFPIAETSGHFHDTYGQALS
                     NIYECLQMGIATFDTSVSGLGGCPYAKGATGNVATEDVVYLLHGLGIKTGIDLDKLVD
                     AGAFISRQIGKPNGSRVARALLAKRADPAAVCA"
     gene            1090..1584
                     /locus_tag="VIN75_14630"
     CDS             1090..1584
                     /locus_tag="VIN75_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8975450.1"
                     /translation="MSDAADRPEGFQRVSQALLRLGHPHAPTYLDIAARTSQEAADAL
                     GVAVGQIAKSVVFRRKSDEVAILVVASGDRRVDEKKVAQIVGPIVRADAEYVKAKTGF
                     TIGGVSPLAHATRVVTLIDRELFRFDRVWAAAGHPHGVFPMTPAELEKLTGAPVHDVV
                     QAPA"
     gene            1754..1942
                     /locus_tag="VIN75_14635"
     CDS             1754..1942
                     /locus_tag="VIN75_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY8975451.1"
                     /translation="MAEIGDATPVPSPCVNICKIDPYTGMCHGCRRTLDEIAAWSALG
                     DADKRWVWQQLPGREPPI"
     gene            1953..2543
                     /locus_tag="VIN75_14640"
     CDS             1953..2543
                     /locus_tag="VIN75_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY8975452.1"
                     /translation="MQRVIFDFDIVSPYAYLAFERLPSALEGVSHHVEYRPLLFAGLL
                     KHWGNTAPVAVAPKKAWLFRQCAWLAQQEGIVFRPPTPHPFNPLALLRLLVASAPAGG
                     QPNRRAVELAFRHVWARDGGDPNDAAALRAVTEAISPVRDPAGAEVKEELQARTADAA
                     AAGVFGVPSFRLDDGQLFWGTDSMAMLADAMRRRQG"
     gene            complement(2585..>4627)
                     /locus_tag="VIN75_14645"
     CDS             complement(2585..>4627)
                     /locus_tag="VIN75_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975453.1"
                     /translation="RAQWEMLDLAALTRDIAGLFRSTAERLGLRLTVDCPPLLSPVPA
                     DRQMWEQIVSNLLANALKHTFEGGITVRLSDIGQHVELSVTDTGVGIPEDELSNIFLR
                     FHRVRGMRSRTDEGVGIGLAMVNELVQLHHGRVRARSRPGQGSTFTVWLSRRQPLHIG
                     GRPAQSRPVRNVALQLAEEADRWAVPAHEPTPAAVVESVLSPARPEMPVRVPDARVLV
                     VDDNADMRDYLARMLGAYWNIVVARDGEEALALARQHRPDLVLTDVMMPGLDGFGLLR
                     RLREDDELKATSVVLVTARAHEQAAIDGLLAGADDYVAKPFASRELVARIGAQLELSR
                     VRRHGERRVRELLALMPVAVYACDRDGRFEIVNRRAIELWGAEPDRGDRRWALCGGPS
                     VRLPDGGSLEPDAAPMATVLSTGETVIDRELVIVRADGRTCDILVNIRPLHDADGRLV
                     GAVSAFLDVSERKRAERALQTMNEELERRVAERTQALAASEAALAADLRDTMLLHRVS
                     SRPDGESEMQSLYLDILDTAIALMEADAGTVQILDVATGDLVILAHRNLDQIYPYFLR
                     VPAGSGTPCGVALGRGQRVFLDFDNPSEPDPHDSNGIHLRGGLRSAQSTPLVTRRGRP
                     IGMFSTHWRDHHRPGERELRYLDLLARQAADLIERQQTLERLQERDGVLTRLQAGT"
BASE COUNT          656 a         1639 c         1690 g          642 t
ORIGIN      
        1 gaagggcgac gccatcaaga ccggacagcc gctggcggtt ctcgaagcga tgaaaatgga
       61 gcacaccatc gccgcgccgc gcgacggcac cgtggccgag ctgctgttcg cgcccggcga
      121 ccaggtcagc gaaggcggcg aactgctcac gttggagtaa gccatgcccc agtttccgga
      181 gttcgtgaag atcgtcgacg tcggcccgcg cgacggcctg cagaacgaga agaaccacgt
      241 ccccgccgcc gacaagatcg cgctggtcag cctgctgcag gacgccggcc tgcgcgaaat
      301 cgaggtcacc agcttcgtca gccccaagtg ggtgccgcag atggccgatg cgctcgacgt
      361 catggccggc atcgcgcgca agccgggcgt gcgctattcg gtgctcacgc ccaacatgaa
      421 gggctgggag gccgcggcgg cgacgcggcc ggacgagatc gtcgtcttcg gcgcggccag
      481 cgaggcgttc agccagaaga acatcaactg ctcgatcgcc gagtcgatcg accgcttcgc
      541 gccggtggtg gcggcggcca aggcggccgg cgtgaaggtg cgcgccgcga tctcctgcgc
      601 ggtgggctgc ccgtacgagg gcgaggtcgc gccgtcgcgc gtgggcatgg tcgcgcgcat
      661 gatgcgcgcg atcggcgtcg accacgtcgg cgtggccgac acgatcggcg tgggcacgcc
      721 gcgcaaggtg cgcgccgcga tggaagccgc gctggaacac ttcccgatcg cggagaccag
      781 cggccacttc cacgacacct acggccaggc gctgtccaac atctacgaat gcctgcagat
      841 gggcatcgcc acgttcgaca ccagcgtgtc cggcctcggc ggctgcccgt acgcgaaggg
      901 cgcgacgggc aacgtcgcga cggaggacgt cgtctacctg ctgcacggcc tgggcatcaa
      961 gacgggcatc gatctggaca agctggtcga cgccggcgcg ttcatctcca ggcagatcgg
     1021 caagcccaac ggctcgcgcg tggcgcgggc gctgctggcc aagcgtgcgg acccggcggc
     1081 ggtgtgcgca tgagcgacgc tgccgatcgt cccgaaggtt tccagcgcgt gtcgcaggca
     1141 ctgttgcgcc tgggtcatcc gcacgcgccg acgtacctcg acatcgcggc ccgcacgtcg
     1201 caggaggccg ccgacgcact cggcgtggcc gtcggccaga tcgccaagag cgtggtgttc
     1261 cggcgcaagt ccgacgaggt cgcgatcctg gtcgtcgcct cgggcgaccg gcgcgtcgac
     1321 gagaagaagg tggcgcagat cgtcggcccc atcgtccgcg ccgacgccga gtacgtgaag
     1381 gccaagacgg gcttcaccat cggcggcgtg tcgccgctgg cgcacgccac gcgggtcgtg
     1441 acgctgatcg accgcgagct gttccgcttc gaccgcgtgt gggccgcggc aggccatccg
     1501 cacggcgtgt tcccgatgac gccggccgag ctcgagaagc tcaccggcgc gccggtgcat
     1561 gacgtcgtgc aggcgccggc gtgaggggcg agatggtccg cgattcctgc cacgcgcggg
     1621 gcgagcgccg ggccgcccca agccggcccg cgttcccccc gggggaagga gcgacgtatt
     1681 cggcgcggga ggggcgcaca tgacgcgcga cggcatcgag cacgcgccgg attcggcgtt
     1741 cttcgtcgcg cgcctggccg agatcggcga cgcgaccccc gtgccctcgc cctgcgtcaa
     1801 catctgcaag atcgacccgt ataccggcat gtgccacggt tgccgccgca cgctcgacga
     1861 gatcgctgcg tggagcgcgc tgggcgacgc ggacaagcgc tgggtctggc agcaactgcc
     1921 cgggcgcgag ccgccgatct aggagcgtcg ccatgcagcg cgtcatcttc gacttcgaca
     1981 tcgtctcgcc ctacgcgtac ctcgcgttcg agcgcctgcc gtccgcgctg gagggcgtgt
     2041 cgcaccacgt cgagtaccgc ccgctgctgt tcgcgggcct gctcaagcac tggggcaaca
     2101 ccgcgccggt ggcggtcgcg ccgaagaagg cctggctgtt ccgccagtgc gcgtggctcg
     2161 cgcagcagga gggcatcgtg ttccggccgc cgacgccgca cccgttcaac ccgctcgcgt
     2221 tgctgcgcct gctggtggcc agcgcgccgg ccggcggcca gccgaaccgg cgcgcggtcg
     2281 aactcgcgtt ccgccacgtg tgggcgcgcg acggcggcga cccgaacgac gcggccgcgc
     2341 tgcgggcggt caccgaagcc atctcgccgg tgcgcgatcc ggccggcgcc gaggtcaagg
     2401 aagaactcca ggcgcgcacc gccgacgcgg cggcggcggg ggtgttcggc gtgcccagct
     2461 tccgcctcga tgacgggcag ctgttctggg gcacggattc gatggcgatg ctggcggacg
     2521 cgatgcgccg ccggcaaggc tgaaatcggt gcgcgccggc gaccggtgat cgccggcgcc
     2581 tgactcaagt acccgcctgc aggcgcgtca gcacgccgtc gcgctcctgc agccgttcca
     2641 gcgtctgctg gcgctcgatg aggtcggcgg cctggcgcgc cagcaggtcc aggtagcgca
     2701 gctcgcgctc gccggggcga tggtggtcgc gccaatgcgt ggagaacatg ccgatcggcc
     2761 ggccgcgccg cgtcaccagc ggcgtcgact gcgccgagcg caggccgccg cgcaggtgga
     2821 tgccgttgga atcgtgcggg tccggctcgg acgggttgtc gaagtcgagg aagacgcgct
     2881 ggccgcgtcc gagcgcgacg ccgcagggcg tgccggagcc cgcgggcacg cgcaggaagt
     2941 acggatagat ctggtccagg ttgcgatgcg cgaggatcac gagatcgccc gtcgccacgt
     3001 cgaggatctg cacggtgccg gcgtcggcct ccatcagcgc gatggcggtg tccaggatgt
     3061 ccaggtacag cgactgcatc tcgctctcgc cgtcgggccg cgagctgacg cggtgcagca
     3121 gcatcgtgtc gcgcaggtcg gcggcgagcg cggcctcgct ggcggccagt gcctgcgtgc
     3181 gctcggccac gcggcgctcg agttcctcgt tcatcgtctg cagcgcccgc tcggcgcgct
     3241 tgcgctcgct gacgtccagg aaggcgctca ccgcgccgac gaggcggcca tccgcatcgt
     3301 gcagtggccg gatgttgacg aggatgtcgc atgtccggcc gtcggcccgg acgatgacca
     3361 gttcgcggtc gatgaccgtc tcgccggtgc tcaggacggt ggccatcggc gcggcgtcgg
     3421 gttcgagcga gccgccgtcg ggcaggcgca cgctggggcc gccgcacagc gcccagcgcc
     3481 ggtcgccgcg atccggttcc gcaccccaca gctcgatggc acgccggttg acgatctcga
     3541 agcgcccgtc gcggtcgcac gcgtagacgg ccaccggcat cagggccagc agctcgcgaa
     3601 cgcggcgttc cccgtggcgg cgcacgcgcg acagctcgag ctgcgcgccg atgcgcgcga
     3661 cgagttcgcg cgaggcgaac ggcttggcca cgtagtcgtc ggccccggcc agcaggccgt
     3721 cgatggccgc ctgctcgtgc gcgcgggccg tcacgagcac caccgacgtg gccttgagct
     3781 cgtcgtcctc gcgtaggcgg cgcagcaggc cgaagccgtc gagccccggc atcatcacgt
     3841 cggtcagcac caggtccggg cggtgctggc gggccagtgc cagcgcctct tccccatcgc
     3901 gggcgacgac gatgttccag tatgcgccca gcatgcgggc gaggtagtcg cgcatgtccg
     3961 cgttgtcatc gacgacgagc acgcgcgcgt ccggcacgcg caccggcatt tccggccggg
     4021 ccgggctgag cacgctctcc acgaccgcgg ccggcgtcgg ctcgtgcgcc ggcaccgccc
     4081 atcgatccgc ttcctccgcc agctgcagcg cgacgttgcg cacgggccgg ctctgcgccg
     4141 ggcggccgcc gatgtgcagg ggctggcgcc ggctgagcca gacggtgaac gtgctgccct
     4201 gacccggcct gctgcgcgcg cgcacacggc cgtggtgcag ctgcaccagc tcgttgacca
     4261 tcgccaggcc gatgcccacc ccctcgtcgg tgcgcgagcg catgccgcgc acgcggtgga
     4321 agcgcaggaa gatgttggac agctcgtcct cggggatgcc cacgccggtg tccgtcacgc
     4381 tcagctccac gtgctggccg atgtcggaca ggcgcacggt gatgccgccc tcgaacgtgt
     4441 gcttcagcgc attggccagc aggttggaga cgatctgttc ccacatctgc cggtcggccg
     4501 gcaccgggga cagcaagggc gggcagtcga cggtcaatcg cagcccgagc cgctccgcgg
     4561 tgctgcggaa aaggccggcg atgtcgcgcg tcaacgccgc caggtccagc atctcccatt
     4621 gcgcgcg
//
LOCUS       DATCRO010000535         8906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_850032, whole genome shotgun sequence.
ACCESSION   DATCRO010000535 DATCRO010000000
VERSION     DATCRO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850032"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VIN75_14650"
     CDS             complement(<1..335)
                     /locus_tag="VIN75_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEY8975454.1"
                     /translation="MTIAPYLREIGRGKDGARSLSREQAADLMGRLLDGKVSDVETGA
                     FAIAMRIKGETPEELAGFMDAVVPRMLALPPPGPAGGVVLPSYNGARKLPNLVPLLAL
                     QLARAGLSVL"
     gene            427..984
                     /locus_tag="VIN75_14655"
     CDS             427..984
                     /locus_tag="VIN75_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975455.1"
                     /translation="MRLLLILIASWFAAAACHAELPTIEAIERQPDHVQAWQSRENYL
                     EPKWGGRLEDVIALAELGHRTVSPGEGRGMMVEVLEIGLDCNCSGLLNHPGIDWEAVR
                     ASMDDVLQRYPDEINAQRCLLIACKRADKALAQHLVPWVKAAPSPFLTDGNVGMFEQR
                     RAWTQGRKASFGVFDAATGRTRIVQ"
     gene            complement(1041..1922)
                     /locus_tag="VIN75_14660"
     CDS             complement(1041..1922)
                     /locus_tag="VIN75_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975456.1"
                     /translation="MTFDHHRTAAHRRPANHLLKPTLALCAALALAGAARAQDAAALH
                     AKFDSLQDRLAHNAYGRPLVLQSTQTGDHLEGEVYARVDQPYAAVQKGLRGTDNWCGI
                     LILHLNVKMCRAQPAGLDMALGRKYDQPVDDAYKLHFDYKSVADGPDYLRAELTSADG
                     PLGTKDYRIAVEAAPLDAAHTMLHMSYAYGFGFTARVAMNTYLSTVGAQKVGFSVTGK
                     DGDGNPEYVGGVRGLVERNTMRYYLAIDDYVAAPAPAQLEQRLATWFDATDRYPRQLH
                     EMEKADYLAMKRIEAKR"
     gene            complement(1919..3319)
                     /locus_tag="VIN75_14665"
     CDS             complement(1919..3319)
                     /locus_tag="VIN75_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8975457.1"
                     /translation="MPALRPHLARAVGSASLAVLAACSTLPTIAPDSAQAAGVENVRI
                     DGPHGPLSDDARKRVLARLQAAGEDTDIFERHLAFEQEITGAPLSSGNKVTLLQDGPS
                     TYQALYAAMAGARNSIDMESYIIEDDEVGRRFAQELKAARARGVVVNLIYDSVGCLHT
                     PKAYFDDLAAAGVNILQFNPIDPLKAKAGWAVNQRDHRKLTIIDGRTAFVGGINISSV
                     YSGGSFSQKSKEQPGHGQKVPWRDTDLQIDGPVVGDFQHLFMDTWSRQKGKPVAPADY
                     YPQVGRQGDAVVRAIGGTPAEPYSQIYATLVSAIRSASTSILLANAYFDPDPQLLGAL
                     EDAARRGVDVQLLLPSVSDTWVVTAAGRRHYGELLESGVKIHERQGALLHSKTTLIDG
                     VWAAVGSTNLDRRSFLHNDEIDAIVLSPAFGDRMRAAFQEDLKQSTPLTLEQWEHRGL
                     WARLQEMSVGMWEYWL"
     gene            3418..4314
                     /gene="purU"
                     /locus_tag="VIN75_14670"
     CDS             3418..4314
                     /gene="purU"
                     /locus_tag="VIN75_14670"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY8975458.1"
                     /translation="MQASPSREREFILRLSCRDTKGIVHAVSGLLFQAGCNIIDSQQF
                     GDLAGDGATGRFFMRVHFEAPPQLADVGTLDRMLSHVTQQFEMDSQLHSLARRPRVML
                     MVSQHGHCLNDLLFRWKSGQLDIDIPAIVSNHTTFADLAASYGLPFHHLPLAPGATPA
                     AKREQEQAVQAIVDKQAVDLVVLARYMQILSPELCGVLENRAINIHHSFLPSFKGAKP
                     YWQAHDRGVKLIGATAHYVTADLDEGPIIEQDVERVDHTLTPEDMTAIGRDVECVVLA
                     RAVRWHVEHRVLRNGRKTVVFN"
     gene            4330..4782
                     /locus_tag="VIN75_14675"
     CDS             4330..4782
                     /locus_tag="VIN75_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8975459.1"
                     /translation="MPNLKARAAVLHASPEDAEAQFYDALRDGDVDRVMAVWADDEDV
                     VCVHPGGARVVGPQAVRAAFESIFASGQLAVHPEHVKAVQAMGCAVHSVLERIDVVTA
                     DGPRTGWIVATNVYVKTGQGWRMVAHHASPGAPDELPEVGEATPSILH"
     gene            4859..5878
                     /locus_tag="VIN75_14680"
     CDS             4859..5878
                     /locus_tag="VIN75_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8975460.1"
                     /translation="MAAVMSPVTTFSHRAPWWLPGGHLQTILPSLFARATIGEAPRFR
                     RERWTTPDQDFIDVDFLVDAPGSDANATARPTLVLFHGLEGSSQSGYAKAFACLARDQ
                     GWSYAVPHFRGCSGELNLAPRAYHSGDYEEVDWILKRMSERRGGPVLAVGISLGGNML
                     LRWAEESGASASRTAAAVAAVCSPIDMAAGGRAIGRGFGRFTYTRMFLSTMKPKALAK
                     LRQHPGLFDERRLRAARDLYEFDNVFTAPLHGFRDTDDYWKRASAKPRLSDIRIPALV
                     LNALNDPFVPGSSLPKQSEVGSHVTLWQPRHGGHVGFASGRLPPGNAMSMPTSVFGWL
                     KSHHR"
     gene            5916..6497
                     /locus_tag="VIN75_14685"
     CDS             5916..6497
                     /locus_tag="VIN75_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY8975461.1"
                     /translation="MDDMVRAALKKWPNVPSCTGWLALDARGDWYMRDDRTQRAGPFP
                     HSKGSRIEHRQLREFIERNYEGTADGAWYFQNGPQRVYVELEAAPHVWRLAARTGAAP
                     EVRSHAGGDGVAVESAWVDERGRLFLATDAGFGLVHTQDMEAAADAIEAGLWSPQTLE
                     FAAMPARFGFCLSPAARWDGHPPREVADAHAAT"
     gene            complement(6560..7147)
                     /locus_tag="VIN75_14690"
     CDS             complement(6560..7147)
                     /locus_tag="VIN75_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8975462.1"
                     /translation="MSEEQHYEEAEHESGIKTPRQLITLVAAAFIVPVLVIIMLTQFV
                     TLENKKSPGSDAFSASAVAARIAPVASDAVFDASAPVVYKTGQQVFEAQCTTCHTAGL
                     LGAPKFGDAAAWGPRVAKGYDALLQSALHGKGNMPAQGGGDFSDFEVGRAVVYMANHG
                     GGKLAEPAAPAAPAASGAASAAAPAASAAASAASR"
     gene            complement(7495..8370)
                     /locus_tag="VIN75_14695"
     CDS             complement(7495..8370)
                     /locus_tag="VIN75_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8975463.1"
                     /translation="MLYPELFKQLEAVRWDMDKDIPWESFDASKLTDEQATTIKMNAI
                     TEWSALPATEMFLRDNRDDSDFCAFMSVWFFEEQKHSLVLMEYLRRFRPELVPTEDEL
                     HAVRFEFDPAPALETLMLHFCGEIRLNHWYRRAAQWHTEPVIKHIYETLARDEARHGG
                     AYLRYMKKALARFGNEAKAAFTKVGVLMASARRTAQALHPTNLHVNANLFPRDTIQSR
                     VPDPNWLEGWLDKQIQFDAVWENKVVERILHNMSLLLERSFVSVQELNRYRKEVTALV
                     AARVAPPASPDPQAA"
     gene            complement(8490..>8906)
                     /locus_tag="VIN75_14700"
     CDS             complement(8490..>8906)
                     /locus_tag="VIN75_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY8975464.1"
                     /translation="LPFQAGLETRGPRILLTTVPGEAHGLGLLMAESLMTLEACRCLA
                     LGPQTPLADIVSAARAHRIDVVALSFSESLPPAQALPALTELRARLPADVAIWAGGGL
                     PALRGLRVAGIRVISRLAEIGGAVADWRRDRSPAAD"
BASE COUNT         1269 a         3120 c         3174 g         1343 t
ORIGIN      
        1 agcaccgaga gcccggcgcg cgcgagctgc agcgccagca gcggcacgag gttgggcagc
       61 ttgcgcgcgc cgttgtacga gggcagcacg acgccgcccg cggggccggg cggcggcagc
      121 gcgagcatgc gcggcacgac ggcgtccatg aagccggcca gctcctcggg cgtctcaccc
      181 ttgatgcgca tggcgatcgc gaacgcgccg gtctcgacgt cgctcacctt tccgtccagc
      241 agccgcccca tcaggtcggc ggcctgctcg cggctgagcg accgcgcgcc gtccttgccg
      301 cggccgatct cgcgcaggta cggggcgatg gtcatgtcga tgcggtgatt cgtcgattca
      361 tgcgccgatt gtggcgcgag cccgggaacg tgtgcccttc gccgcctcga caccccaggg
      421 acttccgtgc gcctcctgct gatcctcatc gcgtcgtggt tcgccgcggc cgcctgccac
      481 gccgaactgc ccaccatcga agccatcgag cgccagccgg accacgtgca ggcctggcag
      541 tcccgcgaga actacctcga gcccaagtgg ggcggccggc tcgaggacgt gatcgcgctg
      601 gccgagctcg ggcaccgcac ggtgtcgccg ggcgagggcc gcggcatgat ggtggaggtg
      661 ctggagatcg gcctggactg caactgcagc ggactgctga accacccggg catcgactgg
      721 gaggcggtcc gagcctcgat ggacgacgtc ctccagcgct acccggacga gatcaacgcg
      781 cagcgctgct tgctgatcgc ctgcaagcgc gcggacaagg cgctggcgca gcacctggtg
      841 ccctgggtga aggcggcgcc gtcgccgttc ctcaccgatg gcaacgtggg gatgttcgag
      901 caacgccgcg cgtggacgca aggcaggaag gcgtcgttcg gcgtgttcga cgcggccacc
      961 ggccggaccc ggatcgtcca gtgaaggcca cgaggcgacg cccgtccgcc ggacgggcgc
     1021 gtgacgcccg cgcgcgatcg tcagcgcttg gcctcgatcc gcttcatcgc gaggtagtcg
     1081 gccttctcca tctcgtgcag ctgccgcgga tagcggtcgg tggcgtcgaa ccaggtggcc
     1141 aggcgctgct cgagctgcgc gggcgccggc gcggcgacgt agtcgtcgat cgccaggtag
     1201 tagcgcatcg tgttgcgctc gacgagcccg cgcacgccgc ccacgtattc cgggttgccg
     1261 tcgccgtcct tgccggtgac gctgaagccg accttctgcg cccccaccgt cgacagatag
     1321 gtgttcatcg ccacccgcgc ggtgaagccg aagccgtagg cgtagctcat gtgcagcatg
     1381 gtgtgggccg cgtccagcgg cgccgcctcg acggcgatgc ggtagtcctt ggtgcccagc
     1441 gggccgtcgg ccgacgtcag ctcggcgcgc aggtagtcgg gcccgtcggc cacggacttg
     1501 tagtcgaagt gcagcttgta ggcgtcgtcg accggctggt cgtacttgcg gcccagcgcc
     1561 atgtccaggc cggccggctg cgcgcggcac atcttcacgt tcaggtgcag gatcaggatg
     1621 ccgcaccagt tgtccgtgcc gcgcaggccc ttctgcaccg ccgcgtaggg ctggtcgacg
     1681 cgtgcgtaga cctcgccctc gaggtggtcg cccgtctgcg tggactgcag caccagcggc
     1741 cggccgtagg cgttgtgcgc gaggcggtcc tgcagcgaat cgaacttggc gtgcagcgcg
     1801 gccgcgtcct gcgcgcgggc ggcgcccgcc agtgcgagcg cggcgcacag cgcgagcgtc
     1861 ggcttgagca agtggttcgc cgggcgacgg tgcgcggcag tgcgatggtg gtcgaaggtc
     1921 atagccaata ttcccacatc cccacggaca tttcctgcag ccgcgcccag agaccgcgat
     1981 gctcccactg ctccagcgtc agcggcgtcg actgcttgag gtcctcctgg aacgccgcgc
     2041 gcatccggtc gccgaaggcc gggctcagca cgatggcgtc gatctcgtcg ttgtgaagaa
     2101 agctgcggcg gtcgaggttg gtcgagccga cggcggccca cacgccgtcg atgagcgtcg
     2161 tcttggaatg cagcagcgcg ccctggcgct cgtggatctt cacgcccgac tccagcagct
     2221 cgccgtagtg gcgccggccc gcggcggtca cgacccaggt gtcgctgacg ctgggcagga
     2281 gcaactgcac gtcgacgccg cggcgcgccg cgtcctcgag cgcgcccagc aactggggat
     2341 cggggtcgaa gtacgcgttg gccagcagga tcgacgtcga cgcgctgcgg atcgccgaga
     2401 cgagcgtcgc gtagatctgg ctgtacggct ctgccggcgt gccgccgatc gcgcgcacga
     2461 cggcgtcgcc ctggcgcccg acctgcgggt agtagtcggc cggcgccacg ggcttgccct
     2521 tctggcgcga ccaggtgtcc atgaacaggt gctggaagtc gccgacgacc gggccgtcga
     2581 tctgcaggtc ggtgtcgcgc cacggcacct tctggccgtg gcccggctgc tccttggact
     2641 tctggctgaa cgagccgccc gaatacacgc tgctgatgtt gatgccgccg acgaaggcgg
     2701 tgcggccgtc gatgatggtc agcttgcgat ggtcgcgctg gttcaccgcc cagccggcct
     2761 tggccttgag cgggtcgatc gggttgaact gcaggatgtt cacgcccgcc gcggccaggt
     2821 cgtcgaagta cgccttgggc gtgtgcaggc agccgacgct gtcgtagatc aggttgacga
     2881 cgacgccgcg cgcccgcgcc gccttgagct cctgcgcgaa gcggcggccg acctcgtcgt
     2941 cctcgatgat gtagctctcc atgtcgatgg agttcctcgc gcctgccatc gcggcataca
     3001 gcgcctggta ggtggacggg ccgtcctgca gcagcgtcac cttgttgccg gacgacagcg
     3061 gcgcgccggt gatctcctgc tcgaacgcga ggtggcgctc gaagatgtcg gtatcctcgc
     3121 ccgcggcctg caggcgcgcc agcacgcgct tgcgcgcgtc gtcggacagc ggcccgtgcg
     3181 ggccgtcgat gcgcacgttc tcgacgcccg cggcctgcgc ggagtccggc gcgatcgtgg
     3241 gcagcgtgct gcacgcggcc agcaccgcga gcgaggcgga gccgacggcg cgggccaggt
     3301 gcgggcggag ggcgggcatc gtcgtcggcg tgggttggag ggaaatcggg gtttcggcga
     3361 agggcggttt ggcaactcgc gtgcccgaca gcaacggggc ctccttacaa tgcgggcatg
     3421 caggcttcac cttcccgcga gcgcgaattc atcctccggc tgtcgtgccg ggacaccaag
     3481 ggcatcgtgc acgcggtgtc gggcctgctg ttccaggccg gttgcaacat catcgactcg
     3541 cagcagttcg gcgacctcgc cggcgacggc gcgacgggcc gcttcttcat gcgcgtgcac
     3601 ttcgaggcgc cgccgcagct ggccgacgtc ggcaccctgg accgcatgct gtcccacgtg
     3661 acgcagcagt tcgagatgga ctcgcagctg cactcgctgg cgcgccgccc gcgcgtgatg
     3721 ctgatggtga gccagcacgg ccactgcctg aacgacctgc tgttccgctg gaagagcggc
     3781 cagctcgaca tcgacatccc ggccatcgtg tccaaccaca cgaccttcgc ggacctggcc
     3841 gccagctacg gcctgccgtt ccaccacctg ccgctcgccc cgggcgccac gccggcggcc
     3901 aagcgcgagc aggagcaggc ggtgcaggcc atcgtcgaca agcaagcggt cgacctcgtc
     3961 gtgctcgcgc gctacatgca gatcctgtcg cccgagctgt gcggcgtgct cgagaaccgc
     4021 gccatcaaca tccaccacag cttcctgccc agcttcaagg gcgccaagcc gtactggcag
     4081 gcgcacgacc gcggcgtgaa gctgatcggc gcgacggccc actacgtcac ggccgacctc
     4141 gacgagggcc cgatcatcga acaggacgtc gagcgcgtcg accacacgct cacacccgag
     4201 gacatgaccg ccatcggacg cgacgtcgag tgcgtcgtcc tcgcgcgcgc ggtgcgctgg
     4261 cacgtcgagc accgcgtgct gcgcaacggc cgcaagaccg tcgtcttcaa ctgagccacc
     4321 ggacgccaga tgcccaacct gaaggcccgc gcggccgtgc tgcacgcctc gcccgaggac
     4381 gccgaggcgc agttctacga tgcgctgcgc gacggcgacg tcgaccgcgt gatggccgtc
     4441 tgggccgacg acgaggacgt cgtgtgcgtg catcccggcg gcgcccgcgt ggtcgggccg
     4501 caggccgtgc gcgccgcgtt cgagtccatc ttcgccagcg gccagctcgc ggtccatccc
     4561 gagcatgtga aggccgtcca ggcgatgggc tgcgccgtgc atagcgtgct cgagcgcatc
     4621 gacgtcgtca ccgccgacgg cccgcgcacg ggctggatcg tcgcgaccaa cgtctacgtg
     4681 aagacgggcc agggctggcg catggtggcg caccacgcga gcccgggtgc gcccgacgag
     4741 ctgcccgaag tcggcgaggc gacgccttcc atcctgcatt gagcgagcgt tgcctgggag
     4801 caacctgggc atgtcgattg cttgccggcc agcgcatact actccccaga cctccgccat
     4861 ggccgccgtc atgtcccccg tcaccacgtt ctcgcaccgc gcgccctggt ggctgcccgg
     4921 cgggcacctg cagaccatcc tgccctcgct gttcgcccgc gcgacgatcg gcgaggcgcc
     4981 ccgcttccgg cgcgagcgct ggacgacgcc ggaccaggac ttcatcgacg tcgacttcct
     5041 cgtcgacgcg cccggcagcg acgccaatgc cacggcgcgc cccacgctcg tgctgttcca
     5101 cggcctggag ggctcgtcgc agagcggcta cgccaaggcc ttcgcatgcc tcgcgcgcga
     5161 ccagggctgg tcgtacgccg tcccgcattt ccgcggctgc tccggcgaac tcaacctcgc
     5221 gccgcgcgcc taccactcgg gcgactacga ggaggtcgac tggatcctca agcgcatgag
     5281 cgagcgccgc ggcggcccgg tgctggccgt gggcatctcg ctgggcggca acatgctgct
     5341 gcgctgggcc gaggagtcgg gcgccagcgc cagccggacc gcggccgccg tggcggccgt
     5401 gtgctcgccg atcgacatgg ccgcgggcgg gcgcgccatc ggccgcggct tcgggcgctt
     5461 cacgtacacg cgcatgttcc tgagcacgat gaagcccaag gcgctggcca agctgcgcca
     5521 gcacccgggc ctgttcgacg agcgccgcct gcgcgccgcg cgcgacctct acgagttcga
     5581 caacgtgttc acggcgccgc tgcacggctt tcgcgacacc gacgactact ggaagcgtgc
     5641 ctcggccaag ccgcgcctga gcgacatccg catcccggcg ctggtgctca acgccttgaa
     5701 cgatccgttc gtcccgggca gctcgctgcc caagcagtcg gaggtcgggt cgcacgtcac
     5761 gctgtggcag ccgcggcacg gcggccacgt cggcttcgcg tcgggccggt tgccgccggg
     5821 caacgcgatg tccatgccga cctccgtctt cggctggctc aagagccacc atcgctgacg
     5881 cgggcgcgcg cccctcgaac gacaggagag ccggcatgga cgacatggtg cgcgccgcac
     5941 tgaagaaatg gcccaacgtg ccgtcctgca cgggctggct cgcgctggac gcgcgcggcg
     6001 actggtacat gcgcgacgac cgcacgcagc gtgccgggcc gttcccgcac tcgaagggca
     6061 gccggatcga gcaccgccag ctgcgcgagt tcatcgagcg caactacgaa ggcacggccg
     6121 acggcgcgtg gtacttccag aacgggccgc agcgcgtgta cgtcgagctc gaggcggcgc
     6181 cgcacgtgtg gcgcctcgcc gcacggaccg gcgccgcgcc cgaggtgcgc tcgcacgcgg
     6241 gcggcgacgg cgtggcggtg gaatcggcct gggtcgacga gcgcgggagg ctgttcctgg
     6301 ccaccgacgc cggcttcggt ctcgtgcaca cccaggacat ggaggccgcc gccgatgcga
     6361 tcgaggccgg cctgtggtcg ccgcagacgc tggagttcgc cgccatgccc gcgcgcttcg
     6421 gtttctgcct gagcccggcg gcccggtggg acggccatcc gccgcgcgag gtggcggacg
     6481 cgcacgcggc gacctgaaca ttgccgccgc ccatgaaaaa aagccgcttg cgggcggctt
     6541 tggtcttgct gcgggcggct tacctggaag cggccgatgc ggctgcggaa gcggccggcg
     6601 cggcagcgga cgccgcgccc gaggccgcgg gcgccgccgg agccgccggc tcggcgagct
     6661 tgccgccgcc gtggttggcc atgtagacga cggcgcggcc gacttcgaag tcgctgaagt
     6721 ccccgccgcc ctgcgccggc atgttgccct tgccgtgcaa cgcggactgc agcagggcgt
     6781 cgtagccctt ggccacgcgc gggccccagg cagccgcgtc gccgaacttc ggcgcgccga
     6841 gcaggcctgc ggtgtggcag gtggtgcact gggcttcgaa gacctgctgg ccggtcttgt
     6901 agacgaccgg ggcgctggcg tcgaacaccg cgtcgctggc caccggcgcg atgcgcgcgg
     6961 cgacggcgga agcggagaac gcgtccgagc cgggggattt cttgttctcg agcgtcacga
     7021 actgcgtgag catgatgatc accagcacgg gaacgatgaa ggcggcggcc accagggtga
     7081 tcagctgccg cggcgtcttg atgccggact cgtgttctgc ttcttcgtag tgctgctctt
     7141 cgctcatgga ttcctcaagg cacgggctgc gggaagttca tgcaccgagg acgcggacct
     7201 gtttcgcgca tactcggccg tcaggtaaaa gccgcgcgat tataggttcc gggggcgggc
     7261 cattccccga tggccgtgcc ttggatgccc cgggacaaac ccccgcttgc caaggcggcg
     7321 acgtcggcgc ttcgcaccag gccggcgtcg ccgcggcgcg cccacccacg tgtcatcctg
     7381 cgcgcagtcg cagggtgacc gttttggcgc ttcacgtgaa tctcgtctcg cggccgcggc
     7441 aagcaaaaac gcccggcgga gccgggcgtc gacagtgcaa gccgcgcggg agcgtcaggc
     7501 cgcctgggga tccggcgaag ccgggggcgc gacgcgcgcc gcgaccagcg cggtcacttc
     7561 cttgcggtag cgattgagtt cctgcaccga cacgaagctg cgctccagca gcaggctcat
     7621 gttgtggagg atgcgctcga cgaccttgtt ctcccacacc gcgtcgaact ggatctgctt
     7681 gtccagccag ccttccagcc agttgggatc gggcacgcgg ctctggatgg tgtcgcgcgg
     7741 gaacaggttg gcgttgacgt gcaggttggt cgggtgcagc gcctgggcgg tgcggcgcgc
     7801 gctggccatc agcacgccga ccttcgtgaa cgcggccttg gcctcgttgc cgaagcgcgc
     7861 gagcgccttc ttcatgtagc gcaggtaggc gccgccgtgg cgggcctcgt cgcgggcgag
     7921 cgtctcgtag atgtgcttga tgacaggctc ggtgtgccac tgcgccgcgc ggcggtacca
     7981 gtggttcagg cggatctcgc cgcagaagtg cagcatcagc gtctcgagcg cgggcgccgg
     8041 gtcgaattcg aagcgcaccg cgtgcagctc atcttcggtg ggcacgagct cggggcggaa
     8101 gcggcgcagg tactccatca gcaccagcga gtgcttctgc tcctcgaaga accagacgct
     8161 catgaaggcg cagaagtcgc tgtcgtcgcg gttgtcgcgc aggaacatct cggtggcggg
     8221 cagcgccgac cactccgtga tggcgttcat cttgatggtg gtggcctgct cgtcggtcag
     8281 cttggacgcg tcgaaggact cccaggggat gtccttgtcc atgtcccaac ggacggcttc
     8341 gagttgcttg aacagttcgg ggtacagcat gctggcgtgg gtctcctgga ccgattttag
     8401 tcggcagtgg gcccggatag tgcgacaaaa gcccgacgat gccgaaggag tctcgggaat
     8461 gcaggggcgc gcccccgaga aagatgcggt caatcggcgg ccgggctgcg atcccgccgc
     8521 cagtcggcca ccgccccgcc gatctccgcg agccggctga tcacccggat tccggcaacc
     8581 cgcaggcctc gcagggcggg caggccgccg ccggcccaga tcgccacgtc ggccggcagg
     8641 cgcgcgcgca gctcggtcag cgcgggcagg gcctgcgcgg gcggcaggct ctccgagaag
     8701 ctcagcgcca ccacgtcgat ccggtgcgcg cgggcggccg acacgatgtc ggccaggggc
     8761 gtctgcggcc ccagcgcgag gcagcggcag gcctccagcg tcatcagcga ctcggccatc
     8821 aacaggccca gcccgtgcgc ctcgccgggc acggtcgtca gcaggatgcg cgggccgcgc
     8881 gtctccagcc cggcctggaa cggcag
//
LOCUS       DATCRO010000536         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_962272, whole genome shotgun sequence.
ACCESSION   DATCRO010000536 DATCRO010000000
VERSION     DATCRO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962272"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VIN75_14705"
     CDS             complement(<1..1645)
                     /locus_tag="VIN75_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEY8975465.1"
                     /translation="MDTDTTGETRGADWFFAQRELVIEGGTVRWTDEQRQAEPLLLTD
                     VRFVARNSARRHGLRLDATPPAGWGERFTLRGQFRQPLLSIRTGHWQTWDGQLYADLP
                     YIDVTRLGQYISLDARIREGNGALRLWADVDDGQVVGGAVDLGLDKVDVSLDKGLQPL
                     VLRAVTGRLSGQLTEETLEFSTTALQFDTADGMRWPGGNLWLQHTPARGRTPEHGALR
                     ADRLDLAALALIADRLPLGDATHRLLDSYGPRGLVEHVDLNWQGSLGAPTRYQAKGRV
                     SGLRIASQPAPTDAAAGPSQQTPAAPVAIPTAATTTVSTTAATAAAAANAAAPHQTHD
                     AGTPGLSGATIDFDATHTGGTATLAIAQGTLEFPGVFEEPVIPVDRLSTQLQWKLDSG
                     NGSAQLQVSKLSFANTDAEGEAQASWRTSDPAVSGGKSRFPGVLDLQGKLTRADGTRV
                     FRYLPTGIPQHTRDYVREAVTKGVASSVDFRVRGDLHDMPFKDPKQGDFRIVAKVSDV
                     NYAYAPPSITNATPSRNGTPARVWPALTGLSGELVFERDS"
     gene            1661..1960
                     /locus_tag="VIN75_14710"
     CDS             1661..1960
                     /locus_tag="VIN75_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975466.1"
                     /translation="MEFSLTVAAHVELRALDVELFEIEPPQRARRHAGHHACEAKGLP
                     LVHVLQHHVAQFEGREKPFGAGGDRSDAHRHADGLASLRLERVTEIADSRHNPRV"
     gene            2103..>3257
                     /locus_tag="VIN75_14715"
     CDS             2103..>3257
                     /locus_tag="VIN75_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEY8975467.1"
                     /translation="MTASFQTGSAETSIGVNQLPDTISPSSPAPQGAFSSYSRFVQRL
                     RRRYAAELALLPPGAPRRESMAVAYEALRQRGDGVGDALRIVRQLVMERLVTLDCDEQ
                     APLAVVTTAVTELAELALDIACQEACRELDAAHGAPLGPEGQRAQLWIVGMGKLGARE
                     LNVSSDIDLIYLYDVDGETQGDADGRGRLSNQEYFARAVKRIYALVGDTTEHGFVFRV
                     DLALRPNGNSGPSVVSLDALEEYFQVQGREWERFAWMKSRVVAPRDVVLAGQAQGLRG
                     AVLPFVFRRYLDYSVFDSLRVLHRQIREQSARRSAGRPERANDVKLSRGGIREIEFTV
                     QLLQVVRGGQFPELRTRPTLDALQRLARANLMPQETADALAAAYEFLRRVE"
BASE COUNT          458 a         1090 c         1211 g          498 t
ORIGIN      
        1 tgctgtcgcg ctcgaacacc agctcgcccg acaggccggt gagcgccggc cagacgcgcg
       61 ccggggtgcc gttgcgcgaa ggcgttgcgt tggtgatcga aggcggcgcg taggcgtagt
      121 tgacgtccga caccttggcg acgatgcgga agtcgccctg cttcgggtcc ttgaacggca
      181 tgtcgtgcag gtcgccgcgc acgcggaagt cgacgctgct cgcgacgccc ttggtgacgg
      241 cctcgcgcac gtagtcgcgc gtgtgctggg ggatgcccgt cggcaggtag cggaacacgc
      301 gcgtgccgtc ggcgcgggtg agcttgccct gcaggtcgag cacgcccgga aagcgcgact
      361 tgccgcccga gacggccgga tcgctggtgc gccagctggc ctgggcctcg ccttccgcgt
      421 cggtgttggc gaagctgagc ttcgacacct gcagctgcgc gctgccattg ccgctgtcga
      481 gcttccattg cagctgcgtg gagagccggt cgacgggaat gacgggctcc tcgaacacgc
      541 cggggaattc gagcgtgcct tgcgcgatgg cgagcgtggc ggtgccgccg gtgtgggtgg
      601 cgtcgaagtc gatggtggcg ccgctcagcc cgggggtgcc ggcgtcgtgg gtctggtgcg
      661 gggctgcggc gttggcagcg gctgcagcgg tggcagccgt ggtggagacg gtcgtggtcg
      721 ccgcggtggg tatcgcgacc ggagccgccg gggtctgctg cgaaggcccc gccgcggcgt
      781 cggtcggcgc cggctgcgag gcgatgcgca gcccgctgac ccggcccttg gcctggtagc
      841 gggtgggtgc gcccagcgag ccctgccagt tcaggtcgac gtgctcgacc aggccgcgcg
      901 gcccgtagct gtcgagcagg cggtgggtgg cgtcgcccag cggcagccgg tcggcgatga
      961 gggcgagcgc ggccaggtcg agccggtcgg cgcgcagcgc gccgtgctcg ggcgtgcggc
     1021 ccctggcggg cgtgtgctgc agccacaggt tgccgccggg ccagcgcatg ccgtcggcgg
     1081 tatcgaactg caaggcggtg gtggagaact ccagcgtctc ctcggtgagc tggccggaaa
     1141 ggcggccggt gaccgcgcgc agaaccagcg gctgcaggcc cttgtcgagc gagacgtcga
     1201 ccttgtcgag ccccaggtcg accgcgcctc cgacgacctg gccgtcgtcc acgtcggccc
     1261 acagacgcag cgcaccgttg ccctcgcgga tgcgcgcatc gagcgagatg tactggccga
     1321 gccgggtgac gtcgatgtag ggcaggtcgg cgtacagctg gccgtcccag gtctgccagt
     1381 ggccggtgcg gatcgacagc agcggctggc ggaactggcc gcgcagcgtg aagcgctcgc
     1441 cccagccggc cggcggcgtg gcgtccaggc gcaggccgtg acgccgggcg ctgttgcgcg
     1501 cgacgaagcg cacgtcggtc agcagcaggg gctctgcctg gcgctgctcg tcggtccagc
     1561 gcacggtgcc gccttcgatg accagctcgc gctgcgcgaa aaaccagtcg gcaccgcggg
     1621 tttctccggt cgtgtcggtg tccatgtgca aaccagccac atggagtttt ccctgacggt
     1681 cgcggcgcac gtcgagctgc gggcgctcga tgtagagctg ttcgaaattg agccgccaca
     1741 gcgagcgcgg cgacacgctg gccaccacgc gtgcgaggcg aagggcctcc cgcttgtcca
     1801 cgtcctgcag caccacgtcg cgcaattcga aggccgggaa aagcccttcg gagcgggcgg
     1861 tgatcgatcc gatgcgcacc ggcacgccga tggccttgct agcctgcgcc tcgagcgcgt
     1921 cacggaaatc gccgattcgc ggcacaatcc acgcgtgtag gacaacgact gacagcgcca
     1981 gcagaagcca tgcagcgatc aagagaccga gcagccagcg cgccgtcaca gcggtgattt
     2041 tgagcagacg tgaaggggaa gacgccgtgt cgttcattga ggatcgcgct gtgtccggaa
     2101 ttatgaccgc cagctttcag acgggttccg cggaaaccag catcggtgtg aaccagttgc
     2161 cagacaccat ttctcccagt tctccggcgc cgcagggcgc cttctcttcg tattcgaggt
     2221 tcgtgcagcg cctgcgccgc aggtatgccg ccgagcttgc attgcttccc ccgggcgcgc
     2281 cgcggcgcga atcgatggcg gtggcctacg aggcactgcg ccagcgcggg gacggcgtcg
     2341 gcgacgctct gcgcattgtg cggcagctcg tgatggagcg gcttgtaact cttgattgcg
     2401 acgagcaggc cccgctggcc gtggtgacca ccgccgtcac cgagctggcc gagctggcgc
     2461 tcgacatcgc gtgccaggag gcctgccgcg aactcgacgc ggcccacggc gccccgctgg
     2521 gccccgaagg ccagcgcgcg cagctgtgga tcgtgggcat gggcaagctc ggcgcgcgcg
     2581 agctcaacgt gtcgagcgac atcgacctga tctacctcta cgacgtcgac ggcgagaccc
     2641 agggcgacgc cgacgggcgc ggccgcctgt ccaaccagga atacttcgcg cgcgccgtga
     2701 agcgcatcta cgcgctggtg ggcgacacca ccgagcacgg cttcgtgttc cgcgtcgacc
     2761 tggcgctgcg gcccaacggc aactcgggcc cgagcgtggt ctcgctcgat gcgctggaag
     2821 agtatttcca ggtgcagggc cgcgagtggg agcgcttcgc ctggatgaag agccgcgtgg
     2881 tcgcgccgcg cgacgtcgtg ctcgcaggcc aggcccaggg cctgcgcggc gcggtgctgc
     2941 ccttcgtgtt ccgacgctac ctcgactaca gcgtgttcga ttctctgcgc gtgctgcacc
     3001 ggcagatccg cgagcagtcg gcacgccgca gcgccgggcg ccccgagcgt gccaacgacg
     3061 tgaagctctc gcgcggcggc atccgcgaga tcgagttcac ggtgcagttg ctgcaggtgg
     3121 tccgcggcgg ccagtttccg gagctgcgca cgcggcccac gctcgacgcg ctgcagcgcc
     3181 tggcgcgcgc caacctcatg ccgcaggaga cggccgacgc gctggccgcg gcctacgagt
     3241 tcctgcgccg cgtcgag
//
LOCUS       DATCRO010000537         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_298415, whole genome shotgun sequence.
ACCESSION   DATCRO010000537 DATCRO010000000
VERSION     DATCRO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298415"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VIN75_14720"
     CDS             complement(<1..552)
                     /locus_tag="VIN75_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8975468.1"
                     /translation="MKTASWPGIVLALSAAVLWGTTGTSQHFAGAHLSSTWIGALRLV
                     IAAAFFAGLVALTERGAAARPRPAGLWQRQLLAGASIAAYNLAFFAGVRLAGVAVGTT
                     VAIGSGPLFAGALQALVTRRAPVPLWWLGTALAIAGGAAIALGGGGAASASLGGLLLC
                     LSAGLSYAVYTLTAKSLSGHASPA"
     gene            complement(606..1277)
                     /locus_tag="VIN75_14725"
     CDS             complement(606..1277)
                     /locus_tag="VIN75_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8975469.1"
                     /translation="MSHPAEPLLSIRSYSGEQTVHEHGYAQVLFAWRGRMDIEVGASA
                     DFSDNSCGLIIPAGVTHAYAASPDLRMVVMDVPMQRGLDRARRFPVTAGVRALVGQAS
                     PDDLLAAILGAPRAGVRRGIALDRLQAELAAALHEPWTTARMAGRCLLSPQRFHARLL
                     ELTGRSPQAWLRGLRLDEAQRLLRRGLTLEAVAARVGYATASALAHALRRDRQVGARS
                     LRDDD"
     gene            1453..2490
                     /locus_tag="VIN75_14730"
     CDS             1453..2490
                     /locus_tag="VIN75_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY8975470.1"
                     /translation="MTFTVTLHPAERAFDVARDETILSAAIRAGVGLPYGCRDGACGS
                     CKSKLKDGRVIHGAHQHKALSEAEEAAGLILTCCATPQTDCVVEARMVVGAGEYPISK
                     MPGRVLSLERPSGDVAVVRVQLPATQPFRYRAGQYVEFILRDGSRRSYSMANAPHNLG
                     TPAAIELHIRHMPGGKFTDHVFGAMKEKDILRMEGPFGSFFLREESDKPIVLLASGTG
                     FAPIKALIEQMHHLGSIRPVRLYWGARRKADLYLDEWLATAKELVPQLEYVPVLSEAA
                     PEDAWTGRTGFVHQAVMADFPDLSGHQVYACGVPVMVESAQRDFVAQCRLPDDEFYAD
                     SFTSEADKHGD"
     gene            2537..2956
                     /locus_tag="VIN75_14735"
     CDS             2537..2956
                     /locus_tag="VIN75_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975471.1"
                     /translation="MPFDTLSHQVAWSLLIGAEIFAVAALWPLSEWATTAWRRRGFVM
                     AVVVGMACFALNWKAFAFARLFTDPARADHVLAWLAVPLFLAFPCAVCVVSGQSLRHA
                     GMAARSARVVALTVAGMTALVAPMAVISAACLGGMCL"
     gene            complement(2978..4195)
                     /locus_tag="VIN75_14740"
     CDS             complement(2978..4195)
                     /locus_tag="VIN75_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975472.1"
                     /translation="MTGLVASGPAQAADVEVLHWWTSGGEAKSVEELKRLLAAQGVTW
                     RDFAVAGGGGDAAMTALKQRVDAGKPPTAAQIKGPAIQEWGRKGVLANLDDVAAAGNW
                     DIKLPRTVAQQMKVDGHWAAVPVNVHRVNWLWINKDLLTKVGGRVPDTWDSFFALADR
                     FKAANIVPLAHGGQPWQDLTTFEIVGLGVGGADFYRQAFVAMDPAAWRSPTMGRVIDT
                     FRRLKAYTDKDAPGRDWNKATEMVIKGRAGMQFMGDWAKGEFLAAGKKPETDFVCTAA
                     PNTWTAYIYNIDSFAMFRLSDPQAVQGQKALATTIMSSKFQEIFNLNKGSIPAAVGSD
                     MTHFDHCGQESSAYFVAASLSNTLVPSFAHKMAQAENHVAAVQAIVARMWDDDSYASA
                     KAQADLVAAASAH"
     gene            complement(4766..5485)
                     /locus_tag="VIN75_14745"
     CDS             complement(4766..5485)
                     /locus_tag="VIN75_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975473.1"
                     /translation="MGAGQVLLEVENLRVAYGGIQAVKGINFKVGEGELVSLIGANGA
                     GKTTTLKAVTGIQPVKDGDVRFLGKSIAGQGAWNLVKQGLVMVPEGRGTFTRMTITEN
                     LLMGAHVRHDNEIEADIDKVFTTFPRLKERATQLAGTMSGGEQQMLAMGRALMARPKV
                     LLLDEPSMGLSPIMVDKIFEVIESIHKQGTTVLLVEQNASRALSIANRGYVMESGEIS
                     MDGQADMLLNDPKVRAAYLGE"
     gene            complement(5606..>5745)
                     /locus_tag="VIN75_14750"
     CDS             complement(5606..>5745)
                     /locus_tag="VIN75_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975474.1"
                     /translation="KLVMGLCDRVTVLDYGKQIAEGTPAEVQGNEKVIEAYLGAGHKP
                     H"
BASE COUNT          816 a         2080 c         1974 g          875 t
ORIGIN      
        1 cgccggggac gcatggcccg acagcgactt ggccgtcagc gtgtagaccg cgtacgacag
       61 gcccgccgat aggcagagca gcaacccgcc caggctggcg cttgcggcgc ctccgccgcc
      121 cagcgcgatg gccgcgccgc ccgcgatggc cagcgccgtg ccgagccacc acagcggcac
      181 cggcgcgcgc cgcgtgacca gcgcctgcag cgcgccggcg aacagcggcc cgctgccgat
      241 ggccaccgtg gtgcccacgg ccacgcccgc cagccgcacg ccggcgaaga acgcgaggtt
      301 gtacgccgcg atggacgcgc ccgcgagcaa ctggcgctgc cacaggccgg ccggtcgcgg
      361 tcgcgccgcc gcgccgcgtt cggtcagcgc caccagcccc gcgaagaacg ccgcggcgat
      421 gaccagccgc agtgcgccga tccaggtcga cgacaggtgt gcgccggcga aatgctggga
      481 ggtgccggtc gtgccccaca gcacggcggc ggatagcgcc aggacgatgc ccggccagga
      541 agcggtcttc atgcgtccat ggtggcgcgc gcgccggccc ggtttgtcga gagactctcg
      601 acttttcagt cgtcgtcgcg aagggaccgg gcaccgactt ggcgatcgcg ccgcagcgcg
      661 tgcgccagcg cgctggccgt cgcatagccg acgcgggcgg ccacggcctc gagcgtgagg
      721 ccgcgccgca gcagccgctg cgcctcgtcc agccgcagcc cgcgcagcca ggcctgcggc
      781 gaccggccgg tcagctccag cagccgcgcg tggaagcgct gcgggctcag caggcagcgc
      841 ccggccatgc gggcggtggt ccacggctcg tgcagcgcgg ccgccagctc ggcctgcagg
      901 cggtccagcg cgatgccgcg ccgcacgccc gcgcgcgggg cgccgaggat ggcggccagc
      961 aggtcgtcgg ggctggcctg gcccaccagc gcgcgcacgc cggccgtcac cggaaagcgc
     1021 cgggcgcggt cgaggccgcg ctgcatcggc acgtccatca ccaccatgcg caggtcgggc
     1081 gaggccgcgt aggcgtgcgt gacgccggcc gggatgatca ggccgcagga gttgtcggag
     1141 aagtcggccg acgcgccgac ctcgatgtcc atgcggccgc gccaggcgaa cagcacctgc
     1201 gcgtagccgt gctcgtgcac ggtctgctcg ccgctgtagg agcggatgct cagcaggggt
     1261 tcggcgggat gggacatgcg tcagtgtagg gcgggttagc gcagcgtaac ccgccattcg
     1321 tcggcatcgc caggcatcga catggcgggt tacgcctgcg gctaacccgc cctgcggcag
     1381 cggcccgccc ggcgcgggat aatccgacat tcggtgccgc gcggcacccc ttgcgtgtcc
     1441 agcctgctcc acatgacctt caccgtcacc ctccaccccg ccgagcgggc cttcgacgtc
     1501 gcccgcgacg agaccatcct gtcggcggct atccgcgccg gtgtgggcct gccctacggc
     1561 tgccgcgacg gcgcctgcgg ctcgtgcaag agcaagctca aggacggccg ggtgatccac
     1621 ggcgcgcacc agcacaaggc cctgtccgag gccgaggagg ccgccggcct gatcctcacc
     1681 tgctgcgcca cgccgcagac cgactgcgtc gtcgaggccc gcatggtggt gggcgccggc
     1741 gagtacccga tcagcaagat gcccggccgc gtgctgagcc tggagcggcc gtcgggcgac
     1801 gtggccgtcg tgcgcgtgca actgcccgcg acacagccgt tccgctatcg cgcgggccag
     1861 tacgtcgagt tcatcctgcg cgacggctcg cgccgcagct actcgatggc caacgcgccg
     1921 cacaacctgg gcacgccggc cgccatcgag ctgcacatcc gccacatgcc cggcggcaag
     1981 ttcaccgacc acgtgttcgg cgcgatgaag gagaaggaca tcctgcgcat ggagggcccg
     2041 ttcggcagct tcttcctgcg cgaggagagc gacaagccca tcgtgctgct cgcctcgggc
     2101 accggcttcg cgccgatcaa ggcgctgatc gagcagatgc accacctggg cagcatccgc
     2161 cccgtgcggc tgtactgggg cgcgcgccgc aaggccgacc tgtacctgga cgaatggctc
     2221 gccacggcca aggaactcgt gccgcagctg gagtacgtgc cggtgctgtc ggaggcggcg
     2281 cccgaagacg cctggacggg ccgcaccggc ttcgtccacc aggccgtgat ggccgatttc
     2341 cccgacctgt cgggccacca ggtctacgcc tgcggcgtgc ccgtcatggt ggagtcggcc
     2401 caacgcgact tcgtggcgca gtgccgcctg cccgacgacg agttctacgc cgacagcttc
     2461 acgtcggaag ccgacaagca cggcgactga aggccgcccg gccttcccgc acgcaccgac
     2521 acgcccgccg ctgccgatgc ccttcgacac gctgtcccat caggtcgcct ggtcactgct
     2581 gatcggcgcc gagatcttcg ccgtcgccgc tctgtggccg ttgtccgaat gggcgacgac
     2641 cgcgtggcgc cggcgcggct tcgtcatggc ggtggtcgtg ggcatggcct gcttcgcgct
     2701 gaactggaag gccttcgcgt tcgcgcgcct gttcacggac cccgcgcggg ccgaccacgt
     2761 gctggcctgg ctcgcggtgc cgctgttcct ggccttcccc tgcgccgtgt gcgtggtctc
     2821 cgggcagtcg ctgcgccatg ccggcatggc ggcacgctcg gcccgcgtcg tcgccctgac
     2881 ggtggccggg atgacggcgc tggtggcgcc gatggcggtg atctcggcgg cctgcctggg
     2941 cgggatgtgc ctgtaggggc ctgcccaaac cgtgggttca gtgcgccgag gcggcggcca
     3001 ccaggtccgc ctgcgccttg gccgacgcgt acgaatcgtc gtcccacatc ctggcgacga
     3061 tggcctgcac cgccgcgacg tggttctccg cctgcgccat cttgtgcgcg aacgacggca
     3121 ccagcgtgtt ggacagcgac gccgccacga agtaggcgct cgattcctgc ccgcagtggt
     3181 cgaagtgcgt catgtcggag ccgacggccg cggggatcga gcccttgttg aggttgaaga
     3241 tctcctggaa cttgctgctc atgatcgtgg tcgccagcgc cttctggccc tgtaccgcct
     3301 gcgggtcgct gaggcggaac atcgcgaagc tgtcgatgtt gtagatgtag gcggtccagg
     3361 tgttgggggc cgcggtgcag acgaagtcgg tttcgggctt cttgccggcg gccaggaact
     3421 cgcccttggc ccagtcgccc atgaactgca tgcccgcgcg ccccttgatc accatctcgg
     3481 tggccttgtt ccagtcgcgg cccggcgcgt ccttgtcggt ataggccttc aggcgccgga
     3541 aggtgtcgat gacgcggccc atcgtcgggc tgcgccaggc cgcgggatcc atcgccacga
     3601 aggcctggcg gtagaagtcg gcgccaccga cgcccaggcc gacgatctcg aaggtggtca
     3661 ggtcctgcca cggctggccg ccgtgcgcca ggggcacgat gttggcggcc ttgaagcggt
     3721 cggccagcgc gaagaacgag tcccaggtgt ccggcacgcg gccgccgacc ttcgtcagca
     3781 ggtccttgtt gatccacagc cagttcacgc ggtgcacgtt cacgggcacg gccgcccagt
     3841 ggccgtcgac cttcatctgc tgcgccacgg tgcgcggaag cttgatgtcc cagttgcccg
     3901 ccgcggccac gtcgtcgaga ttggccagca cgcccttgcg gccccattcc tggatggccg
     3961 ggcccttgat ctgcgccgcg gtcggcggct tgccggcgtc gacgcgctgc ttgagcgccg
     4021 tcatcgcggc gtcgccgccg ccgccggcca ccgcgaagtc ccgccaggtg acgccctggg
     4081 cggccagcag ccgcttgagc tcctcgaccg acttggcctc gccgccggac gtccaccagt
     4141 gcagcacctc cacgtccgcg gcctgcgccg ggccgctggc cacgaggccg gtcatcagtg
     4201 cactcgcccc cacgacgacc caacgccgta atgaaatgtt cagattcacg gttgtctccg
     4261 cccgaaagcg gtcgtccaac gcggaatgtc aggcgccctc tcggaataat ttataaatcc
     4321 aagataacgc tgcgttaatg ttttgaacag atggcgaacc cggattcgcg ggacgtccgg
     4381 gcatccggcg gggcgaagcg tgacgggcgt cacggcccgg ccgcatgctc ggcgacggtc
     4441 gccagggcgc catgaaaacg ccgccggccg caaaggaggt gccaaaggag gtgccacaaa
     4501 ggaggtgcca ggtcttgccg ggcaaatcgg cccgccctga gcgtgttgcc acgcaagacc
     4561 tggcgcccga tctatggcgc ccgatctagg cgccggatcc ccctggtcgg ccgtgcaccg
     4621 ccccgttgcc aggcaagacc tggcacccga aattccgccc cgttaccagg caagacctgg
     4681 cacccgaaag gggcggccaa ggggcgccat gaaaaagggc gcccgcgggc gccctggtcg
     4741 tgcggcggcg cggacgccgc cggcgtcact cgccgaggta cgcggcccgc accttcgggt
     4801 cgttcagcag catgtcggcc tggccgtcca tggagatctc gcccgactcc atcacgtagc
     4861 cgcggttggc gatcgacagc gcgcggctgg cgttctgctc caccagcagc acggtggtgc
     4921 cctgcttgtg gatggactcg atcacctcga agatcttgtc gaccatgatc ggcgacaggc
     4981 ccatcgacgg ctcgtccagc agcagcacct tggggcgcgc catcagcgcg cggcccatcg
     5041 ccagcatctg ctgctcgccg cccgacatcg tgccggccag ctgcgtggcg cgttccttca
     5101 ggcgcgggaa ggtcgtgaac accttgtcga tgtcggcctc gatctcgttg tcgtggcgca
     5161 cgtgcgcgcc catcagcagg ttctccgtga tggtcatgcg ggtgaaggtg ccgcggccct
     5221 cgggcaccat caccaggccc tgcttgacga ggttccacgc gccctggccc gcgatggact
     5281 tgcccaggaa cctgacgtcc ccgtccttga ccggctggat gccggtgacg gccttgagcg
     5341 tggtcgtctt gccggcgccg ttggccccga tcaggctgac cagttcgccc tcgcccacct
     5401 tgaagttgat gcccttgacg gcctggatgc cgccgtaggc gacgcgcagg ttctcgactt
     5461 cgagcagtac ttgtccagcg cccatcacag cccctcgtcc gacgggtaca tcgtccgatc
     5521 ccccgagggg acacgggcct tctcgggagc ggcccgtcgc acggcccgtt caaagagtga
     5581 tttcatcttc attggattcg ccctctcaat gcggcttgtg gccggcgccg aggtaagcct
     5641 cgatcacctt ctcgttgccc tgcacctcgg cgggcgtccc ttccgcgatc tgcttgccgt
     5701 agtccagcac ggtcacgcgg tcgcacaggc ccatcaccag cttga
//
LOCUS       DATCRO010000538         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_950630, whole genome shotgun sequence.
ACCESSION   DATCRO010000538 DATCRO010000000
VERSION     DATCRO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950630"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VIN75_14755"
     CDS             complement(<1..774)
                     /locus_tag="VIN75_14755"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8975475.1"
                     /translation="MSSAPTDFDADQRPDEVEPDDESSEIRAHAVTLEQHGQRLDKLL
                     TAIAPEFSRAHLQHLVADGRVQVDGKVSSTSSAKVKAGMRVQVELVKPAHELAFQPEP
                     IPLDVVYEDEHILVVDKPAGLVVHPAAGNWSGTLLNGLLARDPAAAGLPRAGIVHRLD
                     KDTSGLMVVGRTLPAVTGLMRDIAARVVSRKYLALVHGRLKLDEDTIEAPIGRDPKTR
                     VRMAVIHTGKWAKTDVDLVAYDQGIAAVRCTLHTGRTHQI"
     gene            834..1637
                     /locus_tag="VIN75_14760"
     CDS             834..1637
                     /locus_tag="VIN75_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY8975476.1"
                     /translation="MTFSIAIRRLATVLAPIAVAAALAACGATQAEKDAQTPVEKLYA
                     DAMDDVESANYDRAIKQLDRIEGKAAGSILAQQAQLELAYLYWKTADKTQALATIDRF
                     IKLHPSSPALDYALYLRGIINFNDDLGFLGTLSGQNLAERDQAAARESYQSFKQLTEQ
                     FPDSKYSADARLRMDFTVNMLAEYEVRVAAYYMRRAAYVAAANRAKESVLEFPQTAST
                     AEALSIMVKAYDKLGLTTLRDDAQRVLDKNFPNSDFNEAAKPKAWWKFW"
     gene            complement(1826..3832)
                     /locus_tag="VIN75_14765"
     CDS             complement(1826..3832)
                     /locus_tag="VIN75_14765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8975477.1"
                     /translation="MNEGQEDAAPALVQAVLRALSADGPLAREDDAYLERAGQLSMAG
                     AVAQAIEDRATLVVEAGTGVGKTFAYLVPLLLAGRRALVSTATKNLQDQLFYRDLPRL
                     TEALGVPLSTALLKGRASYLCPYRMERARTEATFADRLAIRALSRIETWAQSTTSGDL
                     SELEGLDERSEVIPLVTSTRENCLGSECPKFAVCPVMKARREAMAADVVVVNHHLFFA
                     DMALREGGVAELLPSVDVAVFDEAHQMVDIGVQFLGQTLATGQAIDLARDLVGAGLQQ
                     ARGLAPWTELAARLELLARELRLAAAGPHAHARGGGTTSTRRPWEQVEADGLLPDALG
                     ALASHCALLVETLEPMQDIGPDFTRLRGRADAYRSLCVRFQGTAPEGQVRWVDLTTHQ
                     ARLVESPLDIGGPLSLQREAVPRAWIFTSATLGDDAGLTWFSEPAGLDDARKLRVDSP
                     FDYAANSCVYVPPRFAAPADAHHAESVGRAAAHCAAALRGRTFVLTTTLRAMRSAAEA
                     LQAELGKLGASLEVLVQGTLPKRAMLERFTRGGCVLVGSQSFWEGIDVPGDALQCVLI
                     DKLPFPPPNDPLIEARVKRLQSQGRDAFNDCFVAEAAVSLKQGAGRLIRTVSDQGLLV
                     ICDSRLRTARYGQRLIDALPPMRQLEDGGQALEFLRQLALTHGD"
     gene            complement(3829..4032)
                     /locus_tag="VIN75_14770"
     CDS             complement(3829..4032)
                     /locus_tag="VIN75_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY8975478.1"
                     /translation="MNESLHSPQRHLIELRMAHAELDQLIDASGEQGSVDELTLKRLK
                     KRRLMLRDQISQLEAELSPPEKA"
     gene            4079..>4827
                     /locus_tag="VIN75_14775"
     CDS             4079..>4827
                     /locus_tag="VIN75_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY8975479.1"
                     /translation="MNTTTQGFRISASTGLHKGDRAYQQDQIELLVHPRIPGCALGLV
                     ADGMGGKSGGRTAADQVVMTARQVFERFAPQRDDPTAMLTSLVVESHLMIKLTAITAE
                     EEPHSTLAAFLVMPSRQAFTVHAGDSRIYHYRGAELVRRTKDHSYVQRLIDEGRLTEE
                     KANTHPQSNLLTGCLGTQQDPPVELNHIDYLEVGDSLMACSDGLWHYLTAREIGAIIN
                     ALSPREATEMLISKARQRARGSGDNLSLVLVR"
BASE COUNT          727 a         1746 c         1645 g          709 t
ORIGIN      
        1 gatctggtgc gtgcggccgg tgtgcagcgt gcagcgcacg gcggcgatgc cctggtcgta
       61 ggccacgagg tcgacgtcgg tcttcgccca cttgccggtg tggatcaccg ccatgcgcac
      121 ccgcgtcttc gggtcgcggc cgatcggcgc ctcgatggtg tcctcgtcga gcttcaggcg
      181 cccgtgcacc agcgcgaggt acttgcggct gaccacccgc gcggcgatgt cgcgcatcag
      241 gcccgtgacc gccggcaagg tgcggcccac caccatcagg ccggacgtgt ccttgtcgag
      301 gcggtgcacg atgcccgcgc gcggcagccc cgcggccgcg gggtcgcgcg cgagcaggcc
      361 gttgagcagc gtgcccgacc agttgccggc ggccggatga accacgagcc cggccggctt
      421 gtcgacgacc aggatgtgct cgtcctcgta gaccacgtcc agcgggatcg gctccggctg
      481 gaacgcgagt tcgtgcgcgg gcttgaccag ttcgacctgc acgcgcatgc ccgccttgac
      541 cttggcggac gacgttgacg aaactttacc gtcgacctgg acgcggccgt cggcgaccag
      601 atgctgcaga tgggcgcgcg aaaactcggg cgcgatggcc gtcaggagct tgtccagccg
      661 ctgtccgtgc tgctccagcg tgacggcgtg ggcgcggatc tcggaggact cgtcatccgg
      721 ttcgacctcg tccggccgtt ggtccgcgtc gaagtctgta ggggcactgc tcatataatt
      781 ccgaggtttc tttccatgtt cgcggcttcg tcgccgcagt gcagcccaag ccaatgacct
      841 tctcgatcgc catccgccgc ctcgccactg tcctggcgcc gatcgctgtc gccgccgccc
      901 tggcggcctg cggcgccacc caggccgaga aggacgccca gacgccggtc gagaagctct
      961 acgccgacgc gatggacgac gtcgagtcgg ccaactacga ccgtgcgatc aagcagttgg
     1021 accgcatcga gggcaaggcc gcgggctcca ttctcgccca gcaggcgcag ctcgagctcg
     1081 cctacctgta ctggaagacg gccgacaaga cgcaggcgct cgccaccatc gaccgcttca
     1141 tcaagctgca tccgtccagc cccgcgctgg actacgcgct gtacctgcgc ggcatcatca
     1201 acttcaacga cgacctcggc ttcctcggca cgctcagcgg ccagaacctg gccgagcgcg
     1261 accaggccgc cgcgcgcgag tcctaccagt cgttcaagca gctcaccgag cagttccccg
     1321 attcgaagta ctcggccgac gcccgcctgc gcatggactt caccgtcaac atgctggccg
     1381 aatacgaagt gcgcgtggcc gcgtactaca tgcgccgcgc agcctacgtg gccgctgcca
     1441 accgcgccaa ggaatcggtg ctcgagttcc cgcagacggc ctccaccgcc gaggcgctgt
     1501 ccatcatggt caaggcctac gacaagctgg gcctgacgac gctgcgcgac gacgcgcagc
     1561 gcgtgctgga caagaacttc cccaacagcg atttcaacga ggccgccaag ccgaaggcct
     1621 ggtggaagtt ctggtgatcc ggtgccggtt cgcctgacat gtcgagggcc gcgcttgcgg
     1681 cccttgtcgt ttccggccgg agccgcgccg cacgtagggc gggttagccg caggcgtaac
     1741 ccgccatgtc ggcatgcgtg acgctggcga acggcgggtt acgcctgcgg ctaacccgcc
     1801 ctacgaaatg cggagctcga cccggtcagt cgccgtgtgt caatgcaagc tgccgcagga
     1861 attccagcgc ctgcccgccg tcctccagct ggcgcatcgg cggcagcgcg tcgatcagcc
     1921 gctggccgta gcgcgcggtg cgcaggcgcg agtcgcagat cacgagcagc ccctggtccg
     1981 acaccgtgcg gatcagccgc cccgcgccct gcttgagcga caccgccgcc tccgcgacga
     2041 agcagtcgtt gaacgcgtcg cggccctgcg actgcaggcg cttgacgcgc gcctcgatca
     2101 gcggatcgtt gggcggcggg aacggcagct tgtcgatcag cacgcactgc aacgcgtcgc
     2161 ccggcacgtc gatgccttcc cagaagctct gcgagcccac cagcacgcag ccgccgcgcg
     2221 tgaagcgctc cagcatcgcg cgcttgggca gcgtgccctg caccagcact tcgagcgacg
     2281 cgcccagctt gccgagctcg gcctgcagcg cctcggccgc agagcgcatg gcgcgcagcg
     2341 tcgtggtgag gacgaaggtg cggccgcgca gcgccgcggc gcaatgcgcg gcggcgcggc
     2401 cgacgctctc ggcatgatgc gcgtccgccg gcgcggcgaa gcgcggcggc acgtagacgc
     2461 aggagttcgc cgcgtagtcg aacgggctgt cgacgcgcag cttgcgcgcg tcgtccaggc
     2521 cggcgggttc ggagaaccac gtcagccccg cgtcgtcacc gagcgtggcc gacgtgaaga
     2581 tccaggcgcg cggcaccgcc tcgcgctgca ggctcagcgg gccgccgatg tccagcggcg
     2641 attcgaccag ccgcgcctgg tgcgtcgtga ggtcgaccca gcgcacctgg ccttccggcg
     2701 ccgtgccctg gaagcgcacg cacaggctgc ggtacgcgtc ggcgcgtccg cgcaggcggg
     2761 tgaagtcggg accgatgtcc tgcatcggct ccagagtctc gaccagcagc gcgcagtgcg
     2821 aggccagcgc cccgagcgcg tccggcagca ggccgtcggc ctcgacctgc tcccacggcc
     2881 gccgcgtgct ggtcgtgccg ccgccgcgcg cgtgcgcatg cgggcccgcg gccgcgaggc
     2941 gcagctcgcg cgcgagcagt tccagccgcg cggccagttc cgtccacggc gcgagtccgc
     3001 gcgcctgctg caggccggcg ccgacgaggt cgcgcgcgag gtcgatcgcc tggccggtgg
     3061 ccagcgtctg gccgaggaac tgcacgccga tgtccaccat ctggtgcgcc tcgtcgaaca
     3121 cggcgacatc caccgacggc agcagctccg cgacgccgcc ctcgcgcagc gccatgtcgg
     3181 cgaagaacag gtggtggttg acgacgacga cgtcggccgc catcgcctcg cggcgcgcct
     3241 tcatcaccgg gcacaccgcg aacttggggc actcgctgcc caggcagttc tcgcgcgtgg
     3301 aggtgaccag cgggatgacc tcggagcgct cgtccaggcc ctccagctcg ctgagatcgc
     3361 cgctggtggt cgactgcgcc caggtctcga tgcgcgacag cgcgcggatg gcgaggcggt
     3421 cggcgaacgt ggcctcggtg cgggcgcgct ccatccgata cgggcacagg tagctggcgc
     3481 ggcccttgag cagcgcggtg gacagcggca cgcccagcgc ctcggtcagc cgcggcaggt
     3541 cgcggtagaa cagttggtcc tgcaggttct tggtggcggt gctgaccagc gcccggcgcc
     3601 cggccagcag cagcggcacc aggtaggcga aggtcttgcc cacgcccgtg ccggcctcga
     3661 cgaccagcgt ggcgcgatcc tcgatggcct gggccaccgc cccggccatg ctcagctgcc
     3721 cggcgcgttc caggtaggcg tcgtcctcgc gggccagcgg gccgtcggcc gagagcgcgc
     3781 gcagcaccgc ctgcaccagg gccggtgccg catcctcttg accttcgttc acgctttttc
     3841 cgggggggac agttcggcct cgagctgcga gatctggtcg cgcagcatga ggcggcgctt
     3901 cttcaggcgc ttgagcgtca gctcgtcgac gctgccctgc tcgcccgagg cgtcgatcag
     3961 ctggtcgagc tcggcatggg ccatgcgcag ctcgatcagg tgacgttgtg gggaatgcag
     4021 ggattcgttc atggcggcag gcccgtgtcg ggtgcaatca cagtttatat tgacggtcat
     4081 gaacacgacc acgcaaggct tccgcatttc ggcctcgact ggcctgcaca agggcgaccg
     4141 cgcctatcag caggatcaga tcgagctgct ggtgcatccg cgtatacccg gatgcgcgct
     4201 cggcttggtc gccgacggca tgggcggcaa gagcggcggc cgcacggcgg ccgaccaggt
     4261 cgtgatgacc gcccgccagg tgttcgagcg cttcgcgccc cagcgcgacg acccgaccgc
     4321 catgctcacc agcctggtcg tcgaatcgca cctgatgatc aagctgacgg ccatcaccgc
     4381 cgaggaggag ccgcacagca cgctggccgc gttcctcgtg atgcccagcc gccaggcctt
     4441 cacggtgcac gccggcgact cgcgcatcta ccactaccgc ggcgccgagc tggtgcgccg
     4501 caccaaggac cactcctacg tgcagcgcct gatcgacgaa ggccgcctga ccgaagagaa
     4561 ggccaacacc cacccgcagt ccaacctgct gaccggctgc ctgggcaccc agcaggatcc
     4621 gccggtcgag ctcaaccaca tcgactacct cgaggtcggc gactcgctga tggcctgcag
     4681 cgacggcctg tggcactacc tgaccgcccg cgagatcggc gccatcatca acgcgctgtc
     4741 cccgcgcgag gccaccgaga tgctgatcag caaggcgcgc cagcgcgccc gcggatccgg
     4801 cgacaacctg tcgctggtgc tggtgcg
//
LOCUS       DATCRO010000539         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_512709, whole genome shotgun sequence.
ACCESSION   DATCRO010000539 DATCRO010000000
VERSION     DATCRO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512709"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2236
                     /locus_tag="VIN75_14780"
     CDS             <1..2236
                     /locus_tag="VIN75_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HEY8975480.1"
                     /translation="RDTETITADEVLEDLQLLKQANFGLIRLFDSSDAVAKLTLQVIK
                     DNNLDIKVHLGAYVIAGNDSFNTAEIARAIKLANDYPDIVEAVSVGNETMVSWSFNKF
                     TPAQIEAFLGTVRAGIQQPVTTDDNWALWAAAPDDLLKAVDFVALHTYSLLDTVSAPG
                     QWDWQQAGVPAAQRAAAMMDASIAKAKSDYTAARSHLDGLGYTAMPIVIGETGWKATP
                     TGGETQRAHPVNQKMYVDRLATWKASAGAPLNIVYFEAFDEPWKGGDDGWGLFDVGRK
                     ARYVIQGLVPQAEWEAGTYTDADALYYIPPVATGPVTASRYTLYAEQANAGEAFPSSP
                     LTWDAWQNGQTSSETEDATTAAEGTKSIKITPTPLDWGWGMTMNLRNGSENLTNFQAA
                     GTLNFSIRTTYAGKIEVGFLTGTSTGSSLYDVYIPIASGQYGYVNDGNWHDVHIPISD
                     ITPHGAMAYGMTDPTKAKLDLSTVTNPFVINDTYGVTGNTAGSTTPIWVDNIYWSNSS
                     GSTPTALTFSSGFADGGRTVEGGAYGGYSGSDIDNWNCGAPAQCGNGGTTTSTASADQ
                     TGFYYYYQTLTPATGEYVGIYLQAPGLTTGLSKTADTAGMQLTDQTTMRFTFGMNPEW
                     WASTTRNVGALLTLGKLYGSGNSACNITLVSIFTPTAQAATAYSLPLSSFSFKTDCGS
                     GLTVAQALAQQPVAQVDFQGDGGTAAIAAGGTSTGANLSVPTTGTPALYPTTLVVNGA
                     VTFQ"
     gene            complement(2285..2704)
                     /locus_tag="VIN75_14785"
     CDS             complement(2285..2704)
                     /locus_tag="VIN75_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975481.1"
                     /translation="MLTQPLKAFVLLFLCGALVEDATIFAIAWLAPDVWFRWFHHAQP
                     AGLGTALLRRAAGQWAAFALVQAIALARWKAQPVWLMVVAGLRASDLLTDLSYIAAVP
                     SLTALGWALLTPPPFLNAAFIAAMALAYRRLVVRPAR"
     gene            2844..>3518
                     /locus_tag="VIN75_14790"
     CDS             2844..>3518
                     /locus_tag="VIN75_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HEY8975482.1"
                     /translation="MSSVSSAATMKVCILDNDHLDPAVADTYVSYGVMTEKMFAAAGV
                     PWQFDRFDTMQGRYPDSFDDYDVVLLTGSKADSFSDEPWVQELRRRVSSLLDQRKKLL
                     GICFGHQLIAHCLGAQVGRAPNGWGMGRMSYDWVGPTPLKPAATGATVNLLASHQDQV
                     LTLPAGATLLARSDFCPIAAYAIDDHVFCVQPHPEFIAAYSAYLLDKRRDKVGEERYA
                     TARAELA"
BASE COUNT          636 a         1309 c         1122 g          451 t
ORIGIN      
        1 ccgcgacacc gagacgatca cggccgacga ggtgctcgag gacctgcagt tgctcaagca
       61 ggcgaacttc ggcctgatcc gcctgttcga cagctcggac gccgtcgcca agctgacgtt
      121 gcaggtgatc aaggacaaca acctcgacat caaggtgcac ctgggcgcct acgtcatcgc
      181 cggcaacgac tcgttcaaca cggccgagat cgcgcgcgcc atcaagctcg cgaacgacta
      241 cccggacatc gtcgaggccg tcagcgtcgg caacgagacc atggtgagct ggtcgttcaa
      301 caagttcacg ccggcgcaga tcgaggcctt cctcggcacc gtgcgcgcgg gcatccagca
      361 gccggtgacc accgacgaca actgggcgct gtgggccgcc gcgccggacg acctgctcaa
      421 ggccgtcgac ttcgtcgcgc tacacaccta ctcgctgctc gacaccgtca gcgcgcccgg
      481 ccagtgggac tggcagcagg ccggcgtgcc cgccgcccag cgcgccgcgg ccatgatgga
      541 cgcctccatc gccaaggcca agtccgacta cacggcggcc cgcagccacc tggacggcct
      601 gggctacacc gcgatgccca tcgtcatcgg cgagacgggc tggaaggcca cgcccaccgg
      661 cggcgagacg cagcgcgcac atccggtcaa ccagaagatg tacgtcgacc gcctcgccac
      721 gtggaaggcc tcggcgggcg ccccgctcaa catcgtctac ttcgaggcct tcgacgagcc
      781 gtggaagggc ggcgacgacg gctggggcct gttcgacgtc ggccgcaagg cgcgctacgt
      841 catccagggc ctggtgccgc aggccgaatg ggaggccggc acctacacgg acgccgacgc
      901 gctgtactac atcccacccg tggccaccgg cccggtgacg gccagccgct acacgctgta
      961 cgccgagcag gccaacgccg gcgaagcctt tccgtcgtcg ccgctcacct gggacgcctg
     1021 gcagaacggc cagacctcca gcgagacgga ggacgcgacg accgccgccg aagggaccaa
     1081 gagcatcaag atcacgccca ccccgctgga ctggggctgg ggcatgacga tgaacctgcg
     1141 caacggctcg gagaacctga ccaacttcca ggccgccggc acgctgaact tcagcatcag
     1201 gacgacctac gccggcaaga tcgaagtggg cttcctcacc ggcacgtcca ccggctcgag
     1261 cctgtacgac gtgtacatcc cgatcgcctc gggccagtac ggctacgtca acgacggcaa
     1321 ctggcacgac gtgcacatcc cgatcagcga catcacgccg cacggtgcca tggcctacgg
     1381 catgaccgat cccaccaagg ccaagctcga cctgtcgacc gtgaccaacc cgttcgtgat
     1441 caacgacacc tacggcgtga ccggcaacac cgcgggcagc accacgccga tctgggtcga
     1501 caacatctac tggtcgaact cgtcgggcag cacgcccacg gcgctgacgt tctcgtcggg
     1561 cttcgccgac ggcggccgca cggtcgaagg cggcgcgtac ggcggctaca gcggcagcga
     1621 catcgacaac tggaactgcg gcgcgcccgc gcaatgcggc aacggcggca cgaccacgtc
     1681 caccgccagc gccgaccaga ccggcttcta ctactactac cagacgctca cgccggccac
     1741 cggcgaatac gtcggcatct acctgcaggc gccgggcctg accacgggcc tgagcaagac
     1801 cgccgacacg gccgggatgc agctgaccga ccagacgacc atgcgcttca cgttcggcat
     1861 gaaccccgag tggtgggcca gcacgaccag gaacgtcggc gcgctgctga cgctgggcaa
     1921 gctgtacggt tccggcaaca gcgcctgcaa catcacgctg gtctcgatct tcacccccac
     1981 cgcgcaggcc gccacggcgt actcgctgcc gctgagctcg ttctcgttca agacggactg
     2041 cggttccgga ctgacggtcg cccaggcgct cgcgcagcaa ccggtggcgc aggtcgactt
     2101 ccagggcgac ggcggcaccg ccgcgatcgc agccggcggc acgtccaccg gcgccaacct
     2161 gagcgtgccg acgacgggga cgccggcgct gtacccgacc acgctggtgg tcaacggcgc
     2221 ggtcacgttc cagtgacgca gcgctgaacg cgctctcgac gacgcccggt ccgccgggcg
     2281 tctctcatcg cgccgggcga acgaccaacc ggcgatacgc gagcgccatc gcggcgatga
     2341 aggccgcgtt caggaacggc ggcggcgtga gcaacgccca gcccagcgcc gtcaaggaag
     2401 gcaccgccgc gatgtacgac aggtcggtca gcaggtcgga cgcgcgcagg cctgcgacga
     2461 ccatcaacca gacgggctgc gccttccatc gcgcgagcgc gatcgcctgc acgagcgcga
     2521 acgccgccca ctggccggcc gcgcgtcgca acaacgcggt cccgagcccg gccggctgcg
     2581 cgtgatggaa ccagcggaac cagacgtccg gcgccagcca ggcgatcgcg aagatggtcg
     2641 cgtcctcgac cagcgcgccg cacaggaaca gcagcacgaa ggccttgagc ggttgcgtca
     2701 gcatgggcag ctccccgagt cgtcacgatc cagcctacgc gcatggaacg ggccggccat
     2761 cccgcaagtc gccggcgtcc ctagcacgac gcggcgccgc gcgcgggcga tcgggctatc
     2821 ggtggcggct taccatctcg tccatgagct ccgtttcctc cgcagcgacc atgaaggtct
     2881 gcatcctcga caacgaccac ctcgaccccg ccgtcgcgga cacctacgtc agctacggcg
     2941 tcatgaccga gaagatgttc gccgccgccg gcgtgccctg gcagttcgac cgcttcgaca
     3001 cgatgcaggg ccgctacccc gattcgttcg acgactacga tgtcgtcctg ctcaccggca
     3061 gcaaggccga ctcgttctcc gacgagccct gggtgcagga actgcgccgc cgcgtctcca
     3121 gcttgctgga ccagcgcaag aagctgctgg gcatctgctt cggccaccag ttgatcgcgc
     3181 actgcctcgg cgcccaggtg ggccgtgcgc ccaacggctg gggcatgggc cgcatgagct
     3241 acgactgggt cggcccgacg ccgctgaagc ccgcggccac cggcgccacc gtcaacctgc
     3301 tggcctcgca ccaggaccag gtgctgacgc tgcccgccgg cgccaccctg ctcgcgcgca
     3361 gcgacttctg cccgatcgcc gcgtacgcga tcgacgacca cgtcttctgc gtgcagccgc
     3421 accccgagtt catcgcggcc tacagcgcct acctgctgga caagcggcgc gacaaggtgg
     3481 gcgaggaacg ctatgcgacg gcgcgcgccg agctggcg
//
LOCUS       DATCRO010000540         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_73391, whole genome shotgun sequence.
ACCESSION   DATCRO010000540 DATCRO010000000
VERSION     DATCRO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73391"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1383)
                     /gene="gap"
                     /locus_tag="VIN75_14795"
     CDS             complement(385..1383)
                     /gene="gap"
                     /locus_tag="VIN75_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8975483.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVQNFSDIEVVGINDLLEPEYLLY
                     MLKHDSVHGRFKGEASVDNGTLIVNGKRIRLTAVKNPAELKWGEVGADIVVESTGLFL
                     TKETCQAHLDAGAKKVIMSAPSKDDTPMFVYGVNDKTYAGQAIISNASCTTNCLAPVA
                     KVLNDTFGIKRGLMTTVHATTATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSDVSVIDLTVELNKEASYDDICNAMKAAADGPMKGVLGYT
                     DEKVVSTDFRGEARTSVFDADAGIALDKTFVKVVAWYDNEWGYSNKVLEMARVVGG"
     gene            complement(1495..3504)
                     /gene="tkt"
                     /locus_tag="VIN75_14800"
     CDS             complement(1495..3504)
                     /gene="tkt"
                     /locus_tag="VIN75_14800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8975484.1"
                     /translation="MANAIRALAMDAVQAANSGHPGAPMGMADIAVALWNRHLKHNPA
                     NPHWHNRDRFVLSNGHGSMLQYALLHLTGYDLPIEELKNFRKLHSKTAGHPEVDVTPG
                     VETTTGPLGQGIANAVGMALAEKLLAAEFNRPGHEIVDHHTYVFLGDGCMMEGISHEA
                     CSLAGAWKLNKLVAIYDDNGISIDGQVQPWFIDDVAARFIAYGWNVIDKVDGHDIEVV
                     DAAIEEALKSATAPTLIVCKTVIGKGSPNRAGTSKAHGEALGADEIALTRKALGWDHP
                     PFEVPADIYAAWDARKSGAEFEAEWKTEFAAYEAAHPELAKEFVRRMMGELPADFAEI
                     AAEAVGTAHDKAETVASRKASQQALEFFTARLPEMLGGSADLTGSNLTNTKSTPSLRF
                     GAEGQVMTTEGRIGRHINYGVREFGMAAIMNGVCLHGGYIPYGGTFLTFSDYSRNAIR
                     MAALMKRRVVHVFTHDSIGLGEDGPTHQSVEHAASLRLIPNLDVWRPADTTETAVAWT
                     MALVQKHRPSALLLSRQNLPYLPKVDADVITQGAYVLAEPSEVGLNRKAQVVLMATGS
                     EVHLALQAQEQLAKLKIAARVVSVPSTSVFDRQTESYKVQVLPRGIPRVAVEAGVTDF
                     WWKYRVDAVVGLDRFGESAPAPDLFKFFDITADNIAAVARKVVGR"
     gene            3676..>3908
                     /locus_tag="VIN75_14805"
     CDS             3676..>3908
                     /locus_tag="VIN75_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8975485.1"
                     /translation="MKALILAAGRGERMRPLTDGTPKPLIEVRGKRLIEWHLEALARA
                     GVREVVVNTAWLEDQFPATLGDGSRHGVTITYS"
BASE COUNT          561 a         1266 c         1369 g          712 t
ORIGIN      
        1 gttcggccag ctggctcggc gccttgcgca ccggattgct ctcgacggcg acgaccgccg
       61 ctcccacggc cgccgtcacc acgatcccca ggctgactgc gctcagcagt cgactcaagc
      121 gtgccatcgc tctctctcca aatggttggt ttcgccccgc gacgcgaggc cttgttatcg
      181 atccgagtct agtgggatcc gggttgcaaa ctcgttgcgc gagtgcgtcc agctggacaa
      241 tccgcctgtg tatttcgcaa acgaacgact ccgttgcggc atacgtcacg cccggcccac
      301 ttctcccctg aaaaggcaaa cggcccgcga gcgggccgtt cgttcagctt cgcgcagggt
      361 tcgcgcgatt cctgcggcgg gagtttagcc gccgacgacg cgcgccatct cgagcacctt
      421 gttcgagtag ccccactcgt tgtcgtacca ggccacgacc ttgacgaagg tcttgtccag
      481 cgcgatgccc gcgtcggcgt cgaacaccga ggtgcgcgcc tcgccgcgga agtcggtgga
      541 gaccaccttc tcgtcggtgt agcccagcac gcccttcatc gggccgtcgg ccgccgcctt
      601 catggcgttg cagatgtcgt cgtagctggc ttccttgttc agctcgacgg tcaggtcgat
      661 caccgagacg tcggacgtcg gcacgcggaa cgacatgccc gtcagcttct tgttcagctc
      721 ggggatcacg acgcccacgg ccttggccgc gcccgtcgac gacgggatga tgttctcgag
      781 gatgccgcgg ccgccgcgcc agtccttgtt ggacgggccg tcgacggtct tttgcgtcgc
      841 ggtggtggcg tgcaccgtgg tcatcaggcc gcgcttgatg ccgaacgtgt cgttgagcac
      901 cttggccacc ggggccaggc agttcgtcgt gcacgacgcg ttggagatga tggcctggcc
      961 ggcgtacgtc ttgtcgttga cgccgtagac gaacatcggc gtgtcgtcct tggacggggc
     1021 cgacatgatg accttcttgg cgccggcgtc caggtgcgcc tggcaggttt ccttggtcag
     1081 gaacaggccg gtggactcga cgacgatgtc ggcgccgacc tcgccccact tgagctcggc
     1141 cgggttcttg acggccgtca ggcggatgcg cttgccgttg acgatcagcg tgccgttgtc
     1201 gaccgaggcc tcgcccttga accggccgtg caccgagtcg tgcttgagca tgtacagcag
     1261 gtactcgggc tccagcaggt cgttgatgcc gacgacctcg atgtcggaga agttctgcac
     1321 ggcggcacgg aacaccatgc gcccgatgcg gccgaagccg ttgatgccga tcttgatggt
     1381 catgtcgatg tctctctagt ctcgaagtaa aggaaatccg ggctcgcgga ccgccaagtc
     1441 tgcctgctgc aggttacgac gcgggaactt tcgaccccgc accgcagcag ggccttacct
     1501 gccgacgacc ttgcgcgcga cggcggcgat gttgtccgcc gtgatgtcga agaacttgaa
     1561 caggtccggc gccggggccg actcgccgaa acggtcgagg ccgacgacgg cgtcgacgcg
     1621 gtatttccac cagaagtcgg tgacgccggc ctcgacggcc acgcgcggga tgccgcgcgg
     1681 cagcacctgc accttgtacg actcggtctg gcggtcgaac acgctggtcg acggcaccga
     1741 gaccacgcgc gcggcgatct tgagcttggc cagctgctcc tgcgcctgca gcgcgaggtg
     1801 cacttccgag ccggtggcca tcagcacgac ctgcgccttg cggttgaggc ccacttcgct
     1861 gggctcggcc agcacgtagg cgccctgggt gatgacgtcg gcgtcgacct tgggcagata
     1921 gggcaggttc tggcgcgaga gcagcagcgc gctggggcgg tgcttctgca cgagcgccat
     1981 cgtccaggcc acggccgtct cggtggtgtc ggccgggcgc cagacgtcca ggttggggat
     2041 caggcgcagg ctggcggcgt gctcgaccga ctggtgggtc gggccgtcct cgcccaggcc
     2101 gatggagtcg tgcgtgaaca cgtgcacgac gcggcgcttc atcagcgcgg ccatgcggat
     2161 cgcgttgcgg ctgtagtcgc tgaacgtgag gaaggtgccg ccgtagggga tgtagccgcc
     2221 gtgcaggcac acgccgttca tgatggcggc catgccgaac tcgcgcacgc cgtagttgat
     2281 gtggcggccg atcctgcctt cggtcgtcat gacctggcct tcggcgccga agcgcagcga
     2341 cggcgtgctc ttggtgttcg tgaggttcga gccggtcagg tcggccgagc cgcccagcat
     2401 ctcgggcagc ctggcggtga agaactcgag cgcctgctgg ctggccttgc gcgaggccac
     2461 ggtctcggcc ttgtcgtgcg cggtgccgac ggcctcggcg gcgatctcgg caaagtcggc
     2521 cggcagctcg cccatcatgc ggcggacgaa ctccttggcc agctccggat gcgcggcctc
     2581 gtaggccgcg aactcggtct tccactcggc ctcgaactcg gcgccggact tcctggcgtc
     2641 ccaggccgcg tagatgtcgg ccggcacctc gaacggcggg tggtcccagc ccagggcctt
     2701 gcgggtcagc gcgatctcgt cggcgcccag cgcctcgccg tgcgccttgg acgtgccggc
     2761 gcggttgggc gagcccttgc cgatgacggt cttgcagacg atcagcgtcg gcgcggtggc
     2821 gctcttgagc gcttcctcga tggcggcgtc cacgacctcg atgtcatggc cgtcgacctt
     2881 gtcgatgacg ttccagccgt aggcgatgaa gcgcgcggcg acgtcgtcga tgaaccaggg
     2941 ctgcacctgg ccgtcgatgg agatcccgtt gtcgtcgtag atcgcgacca gcttgttcag
     3001 cttccacgcg ccggccagcg agcaggcctc gtggctgatg ccttccatca tgcagccgtc
     3061 gcccaggaac acgtaggtgt ggtggtcgac gatctcgtgg ccgggacggt tgaattcggc
     3121 ggcgagcagc ttctcggcca gcgccatgcc cacggcgttg gcgatgccct ggcccagcgg
     3181 gccggtggtg gtctcgacgc ccggcgtgac gtccacctcg gggtggccgg cggtcttcga
     3241 gtgcagcttg cggaagttct tcagttcctc gatcggcagg tcgtagccgg tcaggtgcag
     3301 cagcgcgtac tgcagcatcg agccgtggcc gttggacagc acgaagcggt cgcggttgtg
     3361 ccagtgcggg ttggccgggt tgtgcttcag gtggcggttc cacagggcga cggcgatgtc
     3421 ggccatgccc atcggggcgc cggggtgtcc ggagttggcc gcctggacgg cgtccatcgc
     3481 cagcgcgcgg atggcgttgg ccatcaacgt gggcatcggg gtggcgacgg gggtatcggc
     3541 gggaacgaca ttcgagatct cggacatgga agactctttt tggggccagg atgcggcgga
     3601 ggcggaaccg gcgattttag acgggcgccc ggaaatcgcg gggaaatccc gggccgcagc
     3661 tcttaaactg catcaatgaa ggcactcatt cttgccgcgg gccgcggcga gcgcatgcgc
     3721 ccgctcaccg atggcacgcc caagccgctg atcgaggtgc gcggcaagcg cctgatcgaa
     3781 tggcacctgg aggccctcgc gcgggccggc gtgcgcgagg tcgtcgtcaa cacggcgtgg
     3841 ctggaggacc agtttcccgc gaccctgggc gacggttcgc gccacggcgt gacgatcacg
     3901 tattcgtt
//
LOCUS       DATCRO010000541         8424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_51149, whole genome shotgun sequence.
ACCESSION   DATCRO010000541 DATCRO010000000
VERSION     DATCRO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51149"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1853)
                     /locus_tag="VIN75_14810"
     CDS             complement(69..1853)
                     /locus_tag="VIN75_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8975486.1"
                     /translation="MAGSSSARSPLARLERRLAEAQASRAGSPDALVAGGGRASAPAP
                     EPAPFRDTEVRADVLAHFHQLPSYQALLTVGPEAVYQLPGTLHEKLVALDCGGRQACL
                     VRATMPAADKLALDGTVKAVRGALRARGYGVTGEWVAEPHLLREILRNAGTSGKGGAR
                     GGPMQLFERWVALAVPTGATDLHIEVRGNLGVVRIRVDGRVEPLPDGQRGRYSRKEIV
                     DAIAAGYNSTRKGNNVSQYAADQFVDCMIDLDLPGTAGQLRYQNVKGRLGPKTVVRIL
                     RTGDDNRIRFDAAGYAPSHQRLLRLAGRAGKGIVLLSGVTSSGKSTSLKNFIETLPGL
                     EHKAIYTVEDPIEYEIAGAHQIEVLRDIANDDETRRRYAEVMRALMRSDPDGVMLGEI
                     RDKLTALFALQIAETGHLAMGTVHAHLISNIIPRLTNDQIGVSRQALTGPNILNLLVY
                     QALVPKLCRCCALPTSAALRTDPDAREIEEVLRRRFGLATAGLKWTRPEGCAACQGRG
                     TFGKTIVAEMLQPDRVWLRHVRNNDDDAALQHFRSASNRRFDSPDMTGKTVFEHALYK
                     ALQGEIDVRNCEEFDGFERFEILPRECP"
     gene            complement(2005..2412)
                     /locus_tag="VIN75_14815"
     CDS             complement(2005..2412)
                     /locus_tag="VIN75_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975487.1"
                     /translation="MAHAQTIADYSRAQRALLESAMTQSAARSAGIAAPAPASAASMP
                     AAAIAPPTRVTLPPPVPSIEVSGVFSSSAGAIAEVVVNSTPYLLEAGERVPGTAWEVR
                     AVAVDRVVLGRQGSSVADVAGDRWRIFALPALR"
     gene            complement(2471..3790)
                     /locus_tag="VIN75_14820"
     CDS             complement(2471..3790)
                     /locus_tag="VIN75_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975488.1"
                     /translation="MAVIERLIASTAHGDLLVGLLWRAPTAGQGRRRALLEARALTSD
                     ATHWAQALVGAHVRYGLFQPRASEEGMRLPKGTLAAAACFSRLVGQRAPNAALVLTVP
                     ASAQRKEEKYFVVCLEDGVPVVDVLSNEIDARNALGAEDRPIWSDNPVAYPNCEPADL
                     AWLAAGCDRLARLQPMPVNPWPMLAAGMVTVASLGGWVLVQHLHRADAARKAAAAARA
                     ADPTPRYLAALAGQQGAMGSDRPAVVAAAREIFGYGTWVPGWRLASAECSARTQACTR
                     DWVRRGGTFEDLRRALPEDALEMLVPQGSNVPALDVARTRHPFKIARRSMLDPTRPLR
                     ALQAAISDAGPLLQRWRTADIVLELKRPALWPRVPDVPATFVHPAAVLAGDVDLHNVP
                     GPFIVEALVSAPDFVSWESVRVDLGEGTDARGLLKFSATGVFYVAAH"
     gene            complement(3804..5504)
                     /locus_tag="VIN75_14825"
     CDS             complement(3804..5504)
                     /locus_tag="VIN75_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975489.1"
                     /translation="MSEHVQADHATGAVGAASLHGDRTLAQQGAVAAGSQSDADRRAA
                     EQAVPPVLRRARTAWVGSVSVPMGSGERLPSIFTESVRLNFDDAATGGKVSLRTMADR
                     ITAVTAVPVRLKPDVFGGAEPVARSAPPPARLAMPLPALPQLPGHGAADPVAMPPAVS
                     HDGSVNAVAMRWSGSLQGYLDQITDQLSLSWEYRDGVVVIERLRTEFFEIAALESETD
                     YHLGLSGADQANATSSGNGGGGTSSTASSSNEVSEHGKSNVIGSILAAIGQIIKDVPG
                     SSAVRSDGSGRIAVTTTKETLSKVRDFVRVENESLLRQAQVQFDVYSVTRNESDERGI
                     EWSAVLSALGGAYTGSFASPTTLASSTAAGIGFSILTPTQSGVTNRVTSHLGGSSALL
                     KLLSEYGVSTQHREVSLLALNRTWDRKSSLGGRAYVSATIPGAASTTGVGAPGLVTST
                     VTTGDRYLAQPFILDNDTVVLKFGIGLSSLVNLVNFTSGTGSSQQTVQTPETTAINDQ
                     STVALKAGQILVITGLSRTVSSDDRRTLTDDAPIGLGGSKTQSRLREDFVIFVRPTIL
                     "
     gene            complement(5594..6004)
                     /locus_tag="VIN75_14830"
     CDS             complement(5594..6004)
                     /locus_tag="VIN75_14830"
                     /inference="COORDINATES: protein motif:HMM:NF022136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HEY8975490.1"
                     /translation="MNPLIPLLVAAVAGLAHTASAAEPSLPVAPYAGGASGPEAPPRH
                     WSVLVQDITLERTLQRWGAQAGVRLKWDAQRNFLIGAPDMIEGTFETALKTVLDSAGI
                     RQSDYPLEACIYANDPPLVRITRRGEQAHECEAL"
     gene            complement(6040..7113)
                     /locus_tag="VIN75_14835"
     CDS             complement(6040..7113)
                     /locus_tag="VIN75_14835"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HEY8975491.1"
                     /translation="MRADSPPSLRTRAVFLRAFCAALLMAVAGNASSLTGKRFVGRYD
                     FGYAIRGDRLARPAQVFDDGSGKVYFEARPNQPMPAVFAGRNPALLVLQPEGQYFSAR
                     TSATEFTLVLGAARATVRRGSAVLGDETITPQDDTARLASADGGLPAGVDYDPPADPA
                     LRRVRHEQPIVFAPGVATLRPEVLEALSVLVLRIGPDTAITAFGRNDAGGHDALATSR
                     AEALRNALLARGVAARDIHIAAAEDDDATRPHLHASSLRWTSTEQTRDTASSPAALAP
                     SFDITPADRDIAVSLRRWARASGYEIAWDVDWAAPINGALRVEAPSFVDAVQQVVTGL
                     RAQGYPVQARIDADRVVRFVAPE"
     gene            complement(7230..7814)
                     /locus_tag="VIN75_14840"
     CDS             complement(7230..7814)
                     /locus_tag="VIN75_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY8975492.1"
                     /translation="MGKGRFEAFSDGVLAIIITIMVLELKVPHGDTLAALAEQWPVFL
                     SYVISFIYVGIYWNNHHHLLHAATSVTGGMLWANLNLLFWLSLFPFTTGWMGENHFAA
                     TPSAVYGVALLMAAIAWWILQTLIVRAQGENGVLKRALGSDWKGKLSPWLYLVGIVAS
                     FCVTGLAQAIYLLAALIWLVPDRRIEKALAHEKH"
BASE COUNT         1250 a         2962 c         3008 g         1204 t
ORIGIN      
        1 gtagaactcc gcgcgcatcc tccggaagtc ggccagcgcc acccaggcca ccagggcatg
       61 cggccggctc acgggcactc ccgcggcagg atctcgaacc gctcgaagcc gtcgaactcc
      121 tcgcagttgc gcacgtcgat ctcgccctgc aatgccttgt acagcgcgtg ctcgaagacc
      181 gtcttgccgg tcatgtccgg actgtcgaac cggcggttgc tggccgagcg gaagtgctgc
      241 agcgcggcgt catcgtcgtt gttgcgcaca tgacgcagcc agacgcggtc gggctgcagc
      301 atctccgcca cgatcgtctt gccgaaggtg ccgcgcccct ggcaggccgc gcagccttcc
      361 ggtcgcgtcc acttcaggcc cgcggtggcc aatccgaagc ggcggcgcag cacctcctcg
      421 atctcgcgcg catcggggtc ggtgcgcagg gccgccgacg tcggcagcgc gcagcagcgg
      481 cacagcttgg gaaccagcgc ctggtagacc agcaggttca ggatgttggg gccggtgagc
      541 gcctggcggc tgacgccgat ctggtcgttg gtcaggcgcg ggatgatgtt ggagatcagg
      601 tgggcgtgca ccgtgcccat cgcgaggtgg cccgtctcgg cgatctgcag cgcaaacagc
      661 gccgtgagct tgtcgcggat ctcgcccagc atcacgccgt cggggtcgga gcgcatcaac
      721 gcgcgcatga cttcagcgta gcgacggcgc gtctcgtcgt cgttggcgat gtcgcgcagc
      781 acctcgatct ggtgggcgcc cgcgatctcg tactcgatcg gatcctcgac cgtgtagatc
      841 gccttgtgct cgagcccggg cagggtctcg atgaagttct tcaggctggt ggacttgccc
      901 gagctcgtga cgcccgacaa cagcacgatg cccttgccag cgcgtccggc cagccgcagc
      961 aggcgttggt gactcggcgc atagcctgcg gcgtcgaagc ggatccggtt gtcgtcgccg
     1021 gtccgcagga tgcgcaccac cgtcttcggg ccgagccggc ccttgacgtt ctggtagcgc
     1081 agctggcccg ccgtcccggg gaggtcgagg tcgatcatgc agtcgacgaa ctggtcggcc
     1141 gcatattgcg agacgttgtt gcccttgcgg gtgctgttgt aaccggctgc gatggcgtcg
     1201 acgatctcct tgcggctgta gcgcccgcgc tggccgtcgg gcagcggctc gacgcggccg
     1261 tcgacacgga tgcggaccac gcccaggttg ccgcgcacct cgatgtgcag gtcggtggcg
     1321 ccggtgggca cggccagcgc gacccagcgc tcgaacagct gcatcggccc gcccctggcg
     1381 ccgcccttgc cggacgtgcc ggcgttgcgc aggatctcgc gcagcaggtg cggctcggcc
     1441 acccattccc cggtgacgcc gtagccacgt gcccgcaacg cgccccggac cgccttgacc
     1501 gtgccatcga gcgccagctt gtcggcagcg ggcatcgtcg cccggacgag gcaggcctgg
     1561 cgcccgccac agtccagggc caccagcttc tcgtgcaggg tgccgggcaa ctggtagacg
     1621 gcctccgggc cgaccgtcaa cagcgcctgg taggacggca actggtggaa gtgcgcgagg
     1681 acgtcggcac gcacttcggt gtcgcggaac ggcgcaggct ccggtgcagg cgctgacgcg
     1741 cgcccgccgc cggccacgag cgcgtcgggc gagcccgcgc gcgaagcctg cgcttcggcg
     1801 aggcgccgct cgagccgcgc cagcggggag cgcgcggacg acgatcccgc cacgctcgcc
     1861 aggcgaggcg tcgaggccgg agcgactggc gccgcatcgc tcgacgccgc accgccgccc
     1921 gcaccctgcg tcggtgtcac ggacgtaccc accgattcgg tctgctctcg tcgtcgccac
     1981 atctcgagga tccccatgac cggctcagcg aagcgcgggc agcgcgaaga tccgccaccg
     2041 gtcgccggcc acgtcggcca cgctgctgcc ctggcggccc agcacgacgc gatcgaccgc
     2101 gacggcccgc acttcccagg cggtgccggg aacccgttcg cccgcctcca gcaggtaggg
     2161 cgtgctgttg acgacgacct ccgcgatcgc gcccgcgctc gacgagaaga caccgctcac
     2221 ctcgatgctc ggcacgggcg gtggcagcgt cacgcgtgtc gggggcgcga tggccgccgc
     2281 cggcatcgat gcggccgagg ccggtgcagg cgccgcgatg cccgcagatc gcgcggccga
     2341 ttgggtcatc gcggactcga gcaaggcccg ttgggcgcgg ctgtagtcgg cgatggtctg
     2401 ggcgtgcgcc atgggtgcga ggatcgcgag ggcggcggct gcgaaccggc gtccgcgaga
     2461 cgggaaacag ctagtgggcc gcgacataga agactcctgt ggccgagaac ttgagcaggc
     2521 cgcgcgcgtc cgtgccttcg ccgagatcca cgcggacgga ctcccagctg acgaagtcgg
     2581 gcgccgacac cagcgcctcg acgatgaacg ggccgggcac gttgtgcagg tcgacgtcgc
     2641 ccgcgagcac ggccgccggg tgcacgaagg ttgccggcac gtccggcaca cggggccaca
     2701 gcgcgggccg cttcagttcg agcacgatgt cggccgtgcg ccaacgctgc agcaacggcc
     2761 cggcgtcaga gatcgccgcc tggagcgcgc gcagcggacg cgtcggatcg agcatcgagc
     2821 ggcgcgcgat cttgaacgga tgccgcgtcc gggcgacgtc gagcgccggc acgttgctgc
     2881 cttgcggcac cagcatctcg agggcgtcct cgggcaaggc gcgacgcaag tcctcgaacg
     2941 tgcctccgcg ccgcacccag tcgcgcgtgc acgcctgcgt gcgagcgctg cactccgcgg
     3001 acgccaggcg ccagcccggc acccacgtgc cgtagccgaa gatctcgcgc gccgcggcga
     3061 cgacggccgg gcgatcgctg cccatggcgc cctgctggcc ggccagcgcg gccaggtagc
     3121 gcggcgtcgg atcggccgcg cgcgcggcag ccgccgcctt gcgcgcggcg tccgcgcgat
     3181 gcaggtgctg cacgagcacc cagccgccga gcgaagccac cgtgaccatg cccgcggcaa
     3241 gcatcggcca tgggttgacc ggcatcggct gcaggcgcgc gagccggtcg cagccggcgg
     3301 ccagccaggc gaggtcggcg ggctcgcagt tcgggtaggc cacggggttg tcgctccaga
     3361 tcggccgatc ctcggcgccg agcgcattgc gggcgtcgat ctcgttgctg aggacgtcga
     3421 ccaccggcac gccgtcctcg aggcacacga cgaaatattt ctcttccttg cgctgggcgg
     3481 aggccggcac ggtgagcacg agcgcggcgt tgggtgcgcg ttgaccgacc agccgcgaga
     3541 agcatgccgc ggcggccagc gtgcccttgg gcaagcgcat cccctcttcg gaagcgcgcg
     3601 gctggaacag gccgtagcgg acgtgcgcgc cgaccaacgc ctgggcccag tgcgtcgcgt
     3661 cgctggtgag tgcacgcgcc tcgagcagcg cgcgccgccg cccctgcccg gcggtcggtg
     3721 cgcgccacaa caggcccacg agcaggtccc cgtgcgcggt gctcgcgatc aatcgctcga
     3781 tgacggccat cgtctctcgc cgctcaaagg atggtgggtc gcacgaagat cacgaagtcc
     3841 tcgcgcaggc gcgactgcgt cttgctgcca cccaggccga tcggcgcgtc gtcggtcagc
     3901 gtgcgccggt cgtcgctgga gaccgtgcgg ctcagccccg tgatgaccag gatctgcccc
     3961 gccttcagcg ccacggtcga ctggtcgttg atcgcggtgg tttccggtgt ctgcaccgtc
     4021 tgttgcgatg agcccgtgcc cgaggtgaag ttgaccaggt tgaccaacga cgacagcccg
     4081 atgccgaact tgaggacgac cgtgtcgttg tccaggatga acggctgcgc caggtagcgg
     4141 tcgccggtgg tgaccgtaga cgtgaccagg ccgggtgcgc cgacgcccgt cgtgctggcc
     4201 gcgccgggga tggtggcgct cacgtaggcg cggccgccga ggctggactt gcgatcccag
     4261 gtgcggttca aggccagcag actgacctcg cggtgctgcg tggacacgcc gtactcgctg
     4321 agcagcttga gcagcgcgct gcttccgccc aggtgcgacg tgacgcgatt ggtgacgccc
     4381 gactgcgtgg gcgtgaggat ggagaagccg atgcccgcgg ccgtggagct ggccagcgtc
     4441 gtgggcgatg cgaagctgcc cgtgtaggcg cccccgagcg ccgacaggac ggcgctccat
     4501 tcgatgccgc gttcgtccga ctcgttgcgc gtgacgctgt agacgtcgaa ctgcacttgc
     4561 gcctgacgca gcaggctctc gttctccacg cggacgaagt cgcgcacctt ggacaacgtc
     4621 tccttggtgg tggtgacggc gatgcggccg gagccgtccg agcgcaccgc cgaggatccg
     4681 ggcacgtcct tgatgatctg cccgatggcc gcgaggatgg agccgatgac gttggacttg
     4741 ccgtgctcgc tgacctcgtt gctggaactg gcggtgctgc tggtgccgcc gccgccgttg
     4801 ccggaactgg tggcgttggc ctggtcggcg ccgctcaggc ccaggtggta gtccgtctcg
     4861 ctctcgagcg ccgcgatctc gaagaactcg gtgcgcaggc gctcgatgac gaccacgccg
     4921 tcgcggtact cccacgacag gctcagctgg tccgtgatct ggtcgagata gccctgcagc
     4981 gagcccgacc agcgcatcgc gacggcgttg accgacccgt cgtgcgatac cgctggcggc
     5041 atcgcgacgg gatcggccgc gccatggccc ggcagctgcg gcagcgcagg cagcggcatc
     5101 gccaggcgcg caggcggcgg cgctgaccgg gccaccggct cggccccgcc gaacacgtcc
     5161 ggcttcaggc gcaccggcac ggccgtgacg gccgtgatgc gatcggccat cgtgcgcaga
     5221 ctcaccttgc cgccggtggc ggcgtcatcg aaattcaggc gcacggactc cgtgaagatg
     5281 ctgggcaggc gttcgccact gcccatgggc acgctcaccg acccgaccca ggcggtgcgg
     5341 gctcgccgca gcaccggcgg cacggcctgt tcggccgcgc gccggtcggc atcggattgc
     5401 gagcccgctg caacggcgcc ctgctgcgcc agcgtccggt cgccgtgcaa cgacgcggcg
     5461 ccgacggcgc ccgtcgcatg gtcggcctgc acatgctcgg acaccccggg cgacgcgcaa
     5521 ccggccaacg cgccggccag caatgctgag cccgcgagcc cgcgccaagc cttcgaccgt
     5581 acgatggccc gcatcacagc gcctcgcact cgtgagcctg ctctccgcgg cgcgtgatgc
     5641 gcaccagcgg cgggtcgttg gcatagatgc aggcctccag cggatagtcg ctctggcgga
     5701 tgcccgcgga gtccagcacc gtcttcaagg cggtctcgaa cgtgccctcg atcatgtcgg
     5761 gcgcgccgat caggaagttg cgttgcgcat cccacttcag ccggacgccc gcctgcgcgc
     5821 cccagcgctg gagcgtgcgc tcgagggtga tgtcctgcac cagcacgctc cagtgacgag
     5881 gtggcgcttc agggccggaa gcaccgcccg cgtacggcgc gacggggagc gagggctccg
     5941 cggccgaggc cgtgtgggcg aggccggcca cggcggccac gagcaacggg atcaagggat
     6001 tcatggacgg atccttcctg caatcgatgg agcggccact cactcgggcg cgacgaagcg
     6061 gacgacgcgg tcggcatcga tgcgcgcctg caccggatat ccctgcgcgc gcaggccggt
     6121 cacgacctgc tgcaccgcat cgacgaacga cggcgcctcg acgcgcagcg caccgttgat
     6181 cggcgcggcc caatcgacgt cccaggcgat ctcgtagccg ctggcgcgcg cccagcggcg
     6241 cagcgagacc gcgatgtcac gatccgccgg cgtgatgtcg aacgatgggg cgagcgcggc
     6301 gggcgacgag gcggtgtcgc gcgtctgctc ggtgctcgtc cagcgcaacg acgaagcgtg
     6361 caggtgcggt cgcgtggcgt cgtcgtcctc cgcagcggcg atgtggatgt cgcgggccgc
     6421 cacgccgcgc gcgagcaggg cgttgcgcaa ggcctcggcg cgcgacgtcg ccagcgcgtc
     6481 atggccgccg gcgtcgttgc ggccgaacgc ggtgatggcg gtgtccggtc cgatgcgcag
     6541 cacgagcacc gacagggcct ccagcacctc gggtcgcaac gtggcgacgc cgggcgcaaa
     6601 gacgatgggc tgctcgtgcc ggacgcgtcg cagcgccgga tcggctggtg ggtcgtagtc
     6661 gactccggcg ggcaggccgc cgtctgcgga cgccaggcgc gcggtgtcgt cctgcggggt
     6721 gatcgtctcg tcgccgagca cggcgctccc gcggcggacg gtagcgcgcg cggcacccag
     6781 cacgagcgtg aactcggtgg ccgacgtgcg ggccgagaag tactggccct cgggctgcag
     6841 caccagcaac gccgggttgc gacccgcgaa gacagcgggc atgggctggt tgggccgggc
     6901 ctcgaaatag accttgccgc tcccatcatc gaacacctgc gccggacgcg ccaggcggtc
     6961 gccgcggatg gcatagccga agtcgtagcg gccgacgaag cgcttgccgg tcaggctgga
     7021 cgcgttgccc gccaccgcca tcagcaaggc ggcgcagaag gcgcgcagga acacggcgcg
     7081 ggtgcggagg gatggcggcg aatcggctct catggtcgag acgattctgc tgatgcgccg
     7141 gaaaactgcc cggcgccaaa ccaccctgaa atcgcgcgtt tggcgcgccg cgcaaatcag
     7201 cgcgcgctgc tcaaatcagg gcgcaccctt caatgcttct catgcgccaa cgccttttcg
     7261 atccgccggt cgggcaccag ccagatcagg gccgccagca ggtagatcgc ctgcgcgagc
     7321 ccggtcacgc agaacgacgc cacgatgccc accaggtaca accagggcga gagcttgccc
     7381 ttccagtccg agcccagcgc gcgcttgagc acgccgttct cgccctgcgc gcgcacgatc
     7441 agcgtctgca ggatccacca cgcgatggca gccatcagca aggccacgcc gtagacggcc
     7501 gacggcgtcg ccgcgaagtg gttctcgccc atccagccgg tcgtgaacgg gaacagcgac
     7561 agccagaaca acaggttgag gttggcccac agcatgccgc cggtcacgct ggtggcggcg
     7621 tgcagaaggt ggtggtggtt gttccagtag atgcccacgt agatgaagct gatcacgtag
     7681 ctcaggaaca ccggccactg ctcggcgagc gcggccagcg tgtcgccgtg gggcaccttc
     7741 agctccagca ccatgatcgt gatgatgatg gccagcacgc catcgctgaa tgcctcgaac
     7801 cgacccttgc ccatcaccgc ctcccgacgc tccgtgcgcc gcggatcatt gtcgcaaacg
     7861 gccggcgcat cggacctgcg tccggccggc accgggcatc cgcggaccct cgacgcgcac
     7921 gccgtctcgc cgacaccgga tacgcgcggg cacgcggtcg aggcggtcgc cgtgaattcg
     7981 gggggtgcca cgcgcgtgtt cgggatggct ggcgcatggg cgcgggcgca ccatttttcc
     8041 agggcccctg ctcgacatcg ccgacgcccc gcgtacggcg caccgatcac cgtcgggcaa
     8101 gccgattgcc atcaccaaac cggaaggatc gtgaaatcga tccgcgaatg aagacttgcc
     8161 agggctcgag ggtgcgctgg tccgtgcccg cgggttcaca ctggcctgga gacaaaggtg
     8221 gaaacccggt cgcgccgtcg tcgacgacgc tgcccgatcc gtgtgcccat gaaccgattc
     8281 gacccgctcc gaaaattgct gcatgccgac gaggcggttg cccgttacgc gtcgcagccg
     8341 gccacggtgt tcaccgatgg cgtcttcgac ctgctgcaac gcgggcacat cgcctggctg
     8401 gccgaggccc ggcaactggg cgac
//
LOCUS       DATCRO010000542         8159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1041203, whole genome shotgun sequence.
ACCESSION   DATCRO010000542 DATCRO010000000
VERSION     DATCRO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041203"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VIN75_14845"
     CDS             complement(<1..646)
                     /locus_tag="VIN75_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8975493.1"
                     /translation="MFKNLIAGEWVDGANASRNINPSDTSDVVGEYAQADAAQTREAI
                     AAARRAFPAWSLSTPQQRFDILDAAGTEILARRAELGDLLAREEGKTLPEAIGEVQRA
                     GNIFKFFAGEALRPGGEALPSVRPGVGVEITREPIGVVGIITPWNFPIAIPSWKIAPA
                     LAWGNCVVIKPADLVPGSVWALCEILSRCGLPAGVLNLVMGRGAQVGATMLEDRG"
     gene            complement(821..1543)
                     /locus_tag="VIN75_14850"
     CDS             complement(821..1543)
                     /locus_tag="VIN75_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY8975494.1"
                     /translation="MSPPDLARRRPRSLALELVSALSERIRDGRLNVGDKMPTEAAIM
                     AEFDVSRTVVREALSKLQASGLVQTRHGIGTFVLGLGEDAGFRISREQYETLQDVVAV
                     LELRIGIETEAAALAAQRRTEANLAAMRKALDALGVALEQGSDAVAADFQFHQEVARA
                     TQNDHFADLMTTLGSRIIPRARLEPAQVMTDERREYLRRVNGEHESIYDAIAAQDAEG
                     ARAAMRTHLANSRERRRRSQTA"
     gene            1721..3310
                     /gene="garD"
                     /locus_tag="VIN75_14855"
     CDS             1721..3310
                     /gene="garD"
                     /locus_tag="VIN75_14855"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEY8975495.1"
                     /translation="MTDPHARRAPLAIRMHAADNVAIVANDGGLPAGTRLPEGGPGAG
                     LVLRDKVPQGHKVALADIAEGEAVRRYDVPIGFALKAVPAGSWVHERLIAMPAARALE
                     GLPMATAAPPAVEPLEGHTFQGYRNADGSVGTRNILAITTTVQCVAGVVDVAVRRIKS
                     ELLPLYPNVDDVVGLEHTYGCGVAIDAPDAHIPIRTLRHICLNPNFGGEVMVVSLGCE
                     KLQPDRLLPPGSFPITDERDPALGPETVCLQAEEHVGFMSMIEDIVGRARPHLERLNA
                     RRRETVPASELVVGVQCGGSDAFSGVTANPAVGFCADLLVRAGATVMFSENTEVRDAV
                     EQLTRRAATPEVAQGIVRELAWYDRYLDRGGVDRAANTTPGNKAGGLSNIAEKAMGSI
                     VKSGTSAISHVLSPGDKLRRDQRGLVYAATPASDFICGTLQLAAGMNLHVFTTGRGTP
                     YGLAECPVVKVATRTDLARRWNDLMDVNAGRIADGQATIESLGWELFHLLLDVASGRR
                     TWAEHWQLHNALVLFNPAPVT"
     gene            complement(3327..3962)
                     /gene="tetR"
                     /locus_tag="VIN75_14860"
     CDS             complement(3327..3962)
                     /gene="tetR"
                     /locus_tag="VIN75_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR"
                     /protein_id="HEY8975496.1"
                     /translation="MTNSTERSRLNRDDVLRAALELLDEVGIDALSTRKLAERLGVQS
                     PTLYWHFKSKGALLDAMSAAVMQRSRTPSTPGPDEDWRDWLLADGRSFRKALLAIRDG
                     ARLHAGTRPGEAQRESIEARLRLLCAAGFEPEPATLLLMSIGRFVVGWVLEEQSIQQA
                     DAPPSATLAGPDATQYPLLAAGWAANVGGDADAVFDDALRLIVDGAAARLA"
     gene            3992..5197
                     /gene="tet"
                     /locus_tag="VIN75_14865"
     CDS             3992..5197
                     /gene="tet"
                     /locus_tag="VIN75_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(A)/Tet(B)/Tet(C) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HEY8975497.1"
                     /translation="MHFINDRENVVNRALTIILATLLLDAVGMSLVFPILPGLLRELA
                     GADATTYHYGALLAVYAAMQFLFSPVLGALSDRFGRRPVLLASLAGAAADYVLMALAP
                     NLAWLYVGRVLSGVTGASMSVGTAYVTDVSPEPERAMRYGQMGAAMGLGLVIGPLIGG
                     ALGEWSLRAPFVAAAVMNAVNLAMAWRALPESNQNRTAHLDPRKFNAFSALHRLSGRP
                     PLLPLVGVFAVVALVSQVPGSLWILYGQQRYGWSAFIAGMSLACYGGLHAISQAFVTG
                     PLVARLGDKRALLAGMAADGLGFALMGVATMGWMPFALLPLFAVGGLALPALQALLAR
                     QVDEQHQGELQGTIASLTSLIGVAGPLVVTASFAATQKTWPGSVWLAAAVFYLLALPL
                     LFSRRAREA"
     gene            complement(5204..6259)
                     /locus_tag="VIN75_14870"
     CDS             complement(5204..6259)
                     /locus_tag="VIN75_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8975498.1"
                     /translation="MSDVLQTDCLIIGAGPAGLFQVFELGLREICAHVVEALPFAGGQ
                     CVELYGDKPIYDIPALPACTGRELTDRLLQQIRPFATTFHFDQLVSHVARRDDGRFDV
                     RTKSGLQFDCGAIVLAAGVGAFVPRTLKVAGLEAFEGRQLFHHAERVPAEALAGRHVV
                     VSGATEQALAFATRLCGEVARGAPGAPARVTLIHRRDVFDAEDDTVAAFRGACDAGAL
                     HFVAGQPTGLALDGDRLAALTLLDASGATVSLQADTVVVLQGLSPKLGPLADWGLALE
                     RRQVQVETAKFATSVPGLFAIGDVIHYPGKQRLILSAFHEATLAAAGVAGHLSPGDDG
                     PLEYTTSSPRIHKLLGV"
     gene            complement(6285..6608)
                     /gene="fdxA"
                     /locus_tag="VIN75_14875"
     CDS             complement(6285..6608)
                     /gene="fdxA"
                     /locus_tag="VIN75_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY8975499.1"
                     /translation="MTHVVTEACIRCKYTDCVDVCPVDCFREGPNFLAIDPDECIDCA
                     VCIPECPVNAILPEEDVPADQLHFIKLNADLAKGWPSITKRKPALPDADEWKDKKDKL
                     DQLVK"
     gene            complement(6653..7414)
                     /gene="cobA"
                     /locus_tag="VIN75_14880"
     CDS             complement(6653..7414)
                     /gene="cobA"
                     /locus_tag="VIN75_14880"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY8975500.1"
                     /translation="MTTRVTIVGAGPGSADLITLRGARALASATVVLHDALIDPSLRE
                     MAPAAEWIDVGKRGFCDSTAQARINALLVQHARAGGQVVRLKGGDPSVFGRLDEELEA
                     LAAAGIACEVVPGVTAAIAAAADLKRPLTRRGSGRSVALSTAMTRAGELVARRGADTE
                     VFYMAGRQLAALSRRLIAAGWAPETPVAVVSRAGWPDQHGSDHRVETLPEAVVLHGGR
                     PTVVIVGVGATALAATLSTADEPMTMTPVPAASKP"
     gene            complement(7509..>8159)
                     /locus_tag="VIN75_14885"
     CDS             complement(7509..>8159)
                     /locus_tag="VIN75_14885"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY8975501.1"
                     /translation="AQDHGGRIVQATSLGAEDMVVTDLIARLHLPIAIGTLQTGKLHP
                     QTVALIPRIEERYGLQVEVYRPRKEQVVHFVRANGEDAMYKSIELRKACCGLRKMEPL
                     SRMLDGRAAWVTGLRREQSNNRGTVPFVETESNGRLKVNPLAAWTWNDVWHYIAQHDV
                     PFNPLHDEFMPSIGCAPCTRAIAVGEDFRAGRWWWEDEAAKECGLHATRTAEPVAA"
BASE COUNT         1102 a         2868 c         3002 g         1187 t
ORIGIN      
        1 tgccgcgatc ctccagcatc gtggcgccca cctgcgcgcc gcggcccatg acgaggttga
       61 gcacgccggc gggcaggccg cagcgcgaca ggatctcgca cagcgcccac accgagcccg
      121 gcaccaggtc ggccggcttg atgacgacgc agttgcccca cgccagcgcc ggcgcgatct
      181 tccacgaggg gatcgcgatg gggaagttcc acggcgtgat gatgcccacc acgccgatcg
      241 gctcgcgcgt gatctcgaca cccacgccgg gccgcacgga cggcagtgcc tcgccgccgg
      301 gccgcagcgc ctcgccggcg aagaacttga agatgttgcc ggcgcgctgg acctcgccga
      361 tcgcctcggg cagcgtcttg ccttcctcgc gcgcgagcag gtcgccgagc tcggcgcggc
      421 gcgccaggat ctcggtgccg gcggcgtcga ggatgtcgaa gcgctgctgc ggggtgctca
      481 ggctccacgc ggggaacgcg cggcgcgcgg cggcgatggc ctcgcgcgtc tgcgcggcgt
      541 cggcctgcgc gtattcgccg acgacgtcgc tggtgtccga cgggttgatg ttgcgcgagg
      601 cgttcgcgcc gtcgacccat tcgccggcga tcaggttctt gaacatgctg gatttccttg
      661 aatcttatca gtcatcgtac aacaaacacg caacgtgtag ggcgggttag ccgcaggcgt
      721 aacccgcctt ccgtgggcgt gccgatgtcc aatggacaca agccctgagc atatccggcg
      781 cgcgggcaaa tggcggctcg cccctgcggc caacccgccc ctacgccgtc tgcgaccgcc
      841 tgcgccgctc ccggctgttg gccaggtgcg tccgcatcgc cgcgcgcgcg ccctcggcgt
      901 cctgcgccgc gatcgcgtcg tagatgctct cgtgctcgcc gttgacgcgc cgcaggtatt
      961 cgcggcgctc gtcggtcatc acctgcgcgg gctcgaggcg ggcgcgcggg atgatgcggc
     1021 tgcccagggt ggtcatcagg tcggcgaagt ggtcgttctg cgtggcgcgc gcgacctcct
     1081 ggtggaactg gaaatcggcc gcgacggcgt cgctgccttg ctccagcgcg acaccgagcg
     1141 cgtcgagcgc cttgcgcatc gcagccaggt tggcctcggt gcggcgctgc gcggcgagcg
     1201 cggcggcctc ggtctcgatg ccgatgcgca gttccagcac ggccaccacg tcctgcagcg
     1261 tctcgtactg ctcgcggctg atccggaagc ccgcatcctc gcccagcccg agcacgaagg
     1321 tgccgatgcc gtggcgcgtc tgcaccaggc ccgacgcctg cagcttggac agcgcctcgc
     1381 gcaccacggt gcggctgacg tcgaactcgg ccatgatggc ggcctcggtg ggcatcttgt
     1441 cgcccacgtt caggcggccg tcgcggatgc gctcgctgag cgcggagacg agttccagcg
     1501 cgagggagcg agggcggcgg cgggcgagat cgggcgggct catggcggag gagggcagtc
     1561 agcggcgtcc ggcgagggcg ggccggcacg aatgatcgtg ttgtacgaca actgatgacg
     1621 gactacactc actccatgga ctgtaccaag aaccgtccgc gctcgtgacc ctcgctttca
     1681 tgggatccga ggcgccgcca ggcggttcga gaaggacgag atgaccgatc cccacgcccg
     1741 ccgggcgccg ctcgcgatcc gcatgcatgc cgccgacaac gtggccatcg tcgccaacga
     1801 cggcggcctg ccggccggca cgcgcctgcc cgagggcggc ccgggcgccg gcctggtgct
     1861 gcgtgacaag gtgccgcagg gccacaaggt ggcgcttgcc gacatcgccg agggcgaggc
     1921 ggtgcggcgc tacgacgtcc ccatcggctt cgcgctgaag gccgtccccg ccggcagctg
     1981 ggtgcacgag cggctgatcg cgatgcccgc cgcgcgcgcg ctcgagggcc tgccgatggc
     2041 gaccgccgcg ccccccgccg tcgaacccct cgaaggccat accttccagg ggtatcgcaa
     2101 cgcggacggc tcggtgggca cgcgcaacat cctggcgatc acgacgacgg tgcagtgcgt
     2161 ggccggcgtc gtcgacgtcg ccgtgcgccg catcaagtcc gagctgctgc cgctgtaccc
     2221 gaacgtcgac gacgtcgtcg gcctggagca cacctatggc tgcggcgtgg ccatcgacgc
     2281 gcccgacgcg cacatcccga tccgcacgct gcgccacatc tgcctgaacc cgaacttcgg
     2341 cggcgaggtg atggtggtga gcctgggctg cgagaagctg cagcccgacc gcctgctgcc
     2401 gcccggcagc ttcccgatca ccgacgagcg cgacccagcg ctcggtcccg agacggtctg
     2461 cctgcaggcc gaggaacacg tgggcttcat gtcgatgatc gaggacatcg tcggccgcgc
     2521 caggccgcat ctggagcgat tgaacgcgcg ccgtcgggag acggtgccgg ccagcgagct
     2581 ggtggtgggc gtgcagtgcg gcggcagcga cgcgttctcc ggggtcaccg ccaatcccgc
     2641 ggtcggcttc tgcgccgacc tgctcgtgcg cgccggcgcc acggtgatgt tcagcgagaa
     2701 caccgaggtg cgcgacgcgg tcgaacagct gacgcggcgc gccgccacgc ccgaggtcgc
     2761 gcagggcatc gtgcgcgagc tcgcctggta cgaccgctac ctcgaccgcg gcggcgtcga
     2821 ccgcgccgcc aacacgacgc cgggcaacaa ggcgggcggg ctgtccaaca tcgcggagaa
     2881 ggcgatgggc tcgatcgtca agagcggcac gtcggcgatc tcgcacgtgc tttcgcccgg
     2941 cgacaagctg cgccgcgacc agcgcggcct ggtctacgcc gccacgcccg ccagcgactt
     3001 catctgcggc acgctgcagc tggccgccgg catgaacctg cacgtgttca ccacgggccg
     3061 cggcacgccc tacggcctgg ccgagtgccc ggtggtcaag gtggcgacgc gcaccgacct
     3121 cgcgcgccgc tggaacgacc tgatggacgt caacgccggc cgcatcgccg acgggcaggc
     3181 cacgatcgag tccctcggct gggagctgtt ccatctgctg ctggacgtgg ccagcggacg
     3241 ccgcacgtgg gccgagcact ggcaactgca caacgcgctc gtgctgttca acccggcgcc
     3301 ggtgacctga gccggccgcc gcacgctcac gccagccggg cggccgcgcc gtcgacgatg
     3361 agccgcaggg cgtcgtcgaa gaccgcgtcc gcatcgccgc ccacgttcgc ggcccagccg
     3421 gccgccagca gcgggtactg cgtcgcgtcc gggccggcca gcgtggcgga cggcggtgcg
     3481 tcggcctgct ggatcgactg ctcctcgagc acccagccca cgacgaagcg gccgatcgac
     3541 atcagcagca gcgtcgcggg ctcgggctcg aagccggcgg cgcacaacag gcgcagccgc
     3601 gcctcgatgg actcgcgctg cgcctcgccc gggcgcgtgc cggcatgcag ccgcgcaccg
     3661 tcgcggatgg ccagcagcgc cttgcgaaag ctgcggccgt cggcgagcag ccagtcgcgc
     3721 cagtcctcgt ccggcccggg cgtcgacggg gtgcggctgc gctgcatgac ggcggcagac
     3781 atcgcgtcca gcagcgcgcc cttgctcttg aaatgccagt acagggtggg cgactgcacg
     3841 cccaggcgct cggccagctt gcgcgtgctg agcgcatcga tgccgacctc gtcgagcagt
     3901 tcgagcgccg cacgcaggac atcgtcgcgg ttcaggcgcg atcgttcggt ggaattcgtc
     3961 accctagcaa tgatagagaa gttggggcac aatgcacttt atcaatgata gagagaatgt
     4021 cgtgaatcga gccttgacca tcatcctggc cacgctgctg ctggacgccg tcggcatgag
     4081 cctggtgttc cccatcctgc ccggcctcct gcgcgagctg gccggcgccg acgccaccac
     4141 gtaccactac ggcgcgctgc tggccgtgta tgcggccatg cagttcctgt tctccccggt
     4201 gctgggcgcg ttgagcgacc gcttcgggcg acgtcccgtg ctgctggcgt cgctcgccgg
     4261 tgcggcggcc gactacgtgc tgatggcgct cgcgccgaac ctcgcgtggc tgtacgtcgg
     4321 gcgcgtgctg tcgggcgtca ccggcgcgag catgtcggtc ggcaccgcct acgtcaccga
     4381 cgtcagcccc gagcccgagc gcgcgatgcg ctacgggcag atgggggcgg cgatgggcct
     4441 gggcctggtc atcggcccgt tgatcggcgg cgcgctgggc gagtggtcgc tgcgcgcgcc
     4501 gttcgtcgcg gccgcggtga tgaatgcggt gaatctcgcc atggcgtggc gcgcgttgcc
     4561 cgagtccaac cagaaccgca cggcgcatct ggacccgcgc aagttcaacg cgttctcggc
     4621 gctgcaccgc ctgagcgggc ggccgccgct gctgccgctg gtcggcgtgt tcgcggtggt
     4681 ggcgctggtg tcgcaggtgc ccggctcgct gtggatcctg tacggccagc agcgctatgg
     4741 ctggagcgcg ttcatcgccg gcatgtcgct ggcctgctac ggcggcctgc acgcgatttc
     4801 gcaggccttc gtcaccggcc cgctggtggc gcggctcggc gacaagcgcg cgctcctggc
     4861 cggcatggcg gccgacggac tgggcttcgc gctgatgggc gtggccacca tgggctggat
     4921 gcctttcgca ctgttgcccc tgttcgccgt cggcggcctc gcgctgccgg cgctccaggc
     4981 gttgctggcg cggcaggtcg acgagcagca ccagggcgag ctgcagggca cgatcgcgag
     5041 cctgaccagc ctcatcggcg tcgccggccc gctcgtcgtg acggcgtcct tcgcggcgac
     5101 gcagaagacc tggccgggca gcgtgtggct ggccgcggcc gtgttctacc tgctggcgtt
     5161 gccgctgctg ttcagccgcc gggcgcgcga ggcctgatcc gacctacacg cccagcagct
     5221 tgtggatgcg cgggctgctc gtggtgtatt cgagcggccc gtcgtcgccc gggctgaggt
     5281 ggccggcgac accggccgcc gcgagcgtgg cctcgtggaa ggccgacagg atcaggcgct
     5341 gcttgccggg atagtggatg acgtcgccga tggcgaagag gcccgggacg ctggtcgcga
     5401 acttcgcggt ctccacctgc acctggcgcc gttccagcgc gaggccccag tcggccagcg
     5461 gcccgagctt gggcgacagg ccctgcagca cgacgaccgt gtcggcctgc agcgagacgg
     5521 tggcgcccga ggcgtcgagc agcgtgagtg ccgcgaggcg gtcgccgtcc agcgcgaggc
     5581 ccgtgggctg gccggcgacg aagtgcagcg cgccggcatc gcacgcaccg cggaaggcgg
     5641 cgacggtgtc gtcctccgcg tcgaagacgt cccggcgatg gatgagcgtc acgcgcgcgg
     5701 gggcgcccgg cgcgccgcgc gcgacctcgc cgcacaggcg cgtggcgaac gcgagcgcct
     5761 gctcggtggc gccggagacg acgacgtgcc tgccggccag cgcctcggcg ggcacgcgct
     5821 cggcgtgatg gaacagctgg cggccctcga aggcctcgag gccggccacc ttgagcgtgc
     5881 ggggcacgaa ggcgccgacg cccgcggcca gcacgatggc gccgcaatcg aactgcaggc
     5941 cggacttcgt tcgcacgtcg aagcggccgt cgtcgcggcg cgcgacgtgg ctgaccaact
     6001 ggtcgaagtg gaacgtggtg gcgaacggcc ggatctgctg gagcagccga tcggtcagct
     6061 cgcgcccggt gcacgcaggc agcgcgggga tgtcgtagat cggcttgtcg ccatacagct
     6121 cgacgcactg gccgcccgcg aagggcaggg cctcgacgac gtgggcgcag atctcgcgca
     6181 ggcccagctc gaagacctgg aacaggcccg cggggcctgc gccgatgatc aggcagtcgg
     6241 tctggaggac gtcggacatg gcgacggcgc gagccggccc gcgttcactt gaccagctgg
     6301 tcgagcttgt ccttcttgtc cttccactcg tcggcgtccg gcagcgcggg cttgcgcttg
     6361 gtgatgctgg gccagccctt ggccaggtcg gcgttgagct tgatgaagtg gagctggtcg
     6421 gccggaacgt cttcctcggg caggatcgcg ttgaccgggc actcggggat gcacacggcg
     6481 cagtcgatgc actcgtccgg gtcgatcgcc aggaagttgg ggccttcgcg gaagcagtcg
     6541 acagggcaca cgtccacgca atcggtgtac ttgcaacgga tacaggcttc ggtaacgacg
     6601 tgggtcatgg cagcgaggtg cgtggcgggc agaggctgga gcagccggga ttctaaggct
     6661 tggacgcggc aggaacgggc gtcatggtca tgggttcgtc ggcggtcgac aacgtcgccg
     6721 ccagcgccgt ggcgccgacg cccacgatga cgacggtcgg ccgcccgccg tgcagcacga
     6781 cggcctcggg cagcgtctcg acgcgatggt cgctgccgtg ctggtcgggc cagccggcgc
     6841 gcgagaccac ggccaccggc gtctccggcg cccacccggc ggcgatcagg cggcgcgaca
     6901 gcgcggcgag ctggcggccg gccatgtaga agacctcggt gtcggcgccg cgcctggcga
     6961 ccagttcgcc ggcgcgcgtc atggccgtgc tcagcgcgac gctgcggccg gagccgcggc
     7021 gcgtcagcgg gcgcttcagg tcggcggcgg cggcgatggc ggccgtcacg ccgggcacga
     7081 cctcgcacgc gatgcccgcg gcggccagcg cttcgagctc ctcgtcgagc cggccgaaca
     7141 cgctcgggtc gccgcccttg aggcgcacga cctggccgcc ggcgcgcgcg tgctgcacca
     7201 gcagcgcgtt gatgcgcgcc tgcgcggtcg agtcgcagaa gccgcgcttg ccgacgtcga
     7261 tccactcggc cgcgggcgcc atctcgcgca gcgacgggtc gatcagcgcg tcgtgcagca
     7321 ccacggtcgc gctggcgagc gcgcgcgcgc cgcgcagcgt gatcaggtcg gcggatccgg
     7381 ggccggcgcc cacgatggtc actcgggtcg tcatggtctg ggacggtagg gcagcgggga
     7441 gccgcccgca agcaggaaag gtcgactccg gcgccgcggc gccggatcgg gtgcgggagg
     7501 ccggagcctc aggccgcgac gggctcggcc gtcctggtgg cgtgcaggcc gcattccttg
     7561 gcggcctcgt cttcccacca ccagcggccg gcgcggaagt cctcgccgac ggcgatcgcg
     7621 cgcgtgcagg gcgcacagcc gatgctgggc atgaactcgt cgtgcagcgg gttgaagggc
     7681 acgtcgtgct gggcgatgta gtgccacacg tcgttccatg tccacgcggc cagcgggttg
     7741 accttgaggc ggccgttgga ctcggtctcg acgaagggca cggtgccgcg gttgttcgac
     7801 tgctcgcggc gcaggcccgt gacccacgcg gcgcggccgt cgagcatgcg cgacagcggt
     7861 tccatcttgc gcaagccgca gcaggccttg cgcagctcga tgctcttgta catcgcgtcc
     7921 tcgccgttgg ctcggacgaa gtgcacgacc tgttccttgc gcggccggta cacctcgacc
     7981 tgcagcccgt agcgctcctc gatgcgcggg atcagcgcca ccgtctgcgg atgcagcttg
     8041 cccgtctgga gcgtgccgat cgcgatcggc aggtgcaggc gcgcgatcag gtcggtgacg
     8101 accatgtcct cggcgcccag gctggtggcc tgcacgatgc ggccgccgtg gtcctgcgc
//
LOCUS       DATCRO010000543         6017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_478764, whole genome shotgun sequence.
ACCESSION   DATCRO010000543 DATCRO010000000
VERSION     DATCRO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478764"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..601
                     /locus_tag="VIN75_14890"
     CDS             95..601
                     /locus_tag="VIN75_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY8975502.1"
                     /translation="MTGLHDLVLALFGEDQDLNVLQMAMRALAVFVLTLAMIRIAGRR
                     SLGQHRAFDTCTTVLLGSVLSRAVVGASPFWPTMAAGAVFVVLHRLVAMASVRWPRFE
                     TMISGDKRELVQDGRRDEAAMRKALITVRDLDEAVRRKTGDETSQVERAILERDGGIT
                     VKVAEPPG"
     gene            complement(624..1310)
                     /gene="pdeM"
                     /locus_tag="VIN75_14895"
     CDS             complement(624..1310)
                     /gene="pdeM"
                     /locus_tag="VIN75_14895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY8975503.1"
                     /translation="MSTTQSLVLQGRSLSLLPERAAWHAASRTLFIADLHLAKSASFR
                     AQGLPVPAGTTRDNLDRIATLVRTHGAARVVFLGDLLHSRHAQRASAIAPLRAWRDAH
                     PDLHCVLVRGNHDSHAGDPPPSLRFEIVDEPWPVPGAAGLVGCHHPRQLAGAAVLAGH
                     WHPAVTLRGPARDVERLACFCHIADDRADVLVLPAFGAFTGSSPQAPPSGSTCWPVGG
                     GRVWPGMRID"
     gene            complement(1307..3937)
                     /locus_tag="VIN75_14900"
     CDS             complement(1307..3937)
                     /locus_tag="VIN75_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY8975504.1"
                     /translation="MADAAAVEAWLATRGWHPFAFQREVWQAMAEGRSGLLHANTGTG
                     KTLAAWLGALQALRVQASASASRSKKAAAPPVSVLWLTPMRALAADTTRALADPMAAL
                     EPTWSIGLRSGDTTSAERSAQDRRLPTVLVTTPESLTLQLARADALQALAHVQLVVVD
                     EWHELLGNKRGVQVQLALARLRQRRPGLMCWALSATLGNLEEALATLVGVRPRARVTP
                     APALVRGDVAKTIVIDTLLPERAERFSWGGHMGLRMLPQVVQAIDDSGTTLAFTNTRS
                     QAERWYQALLDARPDWAGIVALHHGSLAREVREWVEAGLKSGTLRAVVCTSSLDLGVD
                     FLPVDRVLQIGSAKGIARLLQRAGRSGHAPGRPSRVTLVPTHSLELVEAAAARHAAQQ
                     GQVEARHSPQEPLDVLVQHLVTVALGGGFMPDALYAEVRTTTAYRDLSPAAWQWCLDF
                     VRQGGPSLAAYPDYQRVVPDDAGIWRVPDARLARRHRVNIGTIVSDASINVAYQTGGN
                     LGSVEESFIARMKPGDAFLFGGRLLELVRVHQMTAYVKRAPAGRGAVPRWNGGRMPLS
                     NTLADAVLRELDAADRGRFDSPEMQCVRPLLEIQGRWSALPTPSTLLAETWKSREGWH
                     LFLYPFAGRLVHLGLASLLAWRAARPEQATFSIAVNDYGLELLSAREIAWGERLPALL
                     ATPDDPAALLGEVLASLNATEMSRRRFREIARIAGLIFQSHPGERQSNRQLQASASLF
                     FDVFRRYDPGNLLLTQAERELLRQELDVDQLAAALRRMQGQRLAAVTLARPSPLAFPL
                     MVERFREKLTNESLAERIARMVSDLEGRAGGDAVGTAHEDAKRVFARDALQQAAQASV
                     IADRDEPKPKRRTRRAKLRT"
     gene            complement(3937..5583)
                     /locus_tag="VIN75_14905"
     CDS             complement(3937..5583)
                     /locus_tag="VIN75_14905"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8975505.1"
                     /translation="MKEFAALFAALDSTTSTRRKTEALLAYFQRAQPADAAWALYFLA
                     GGKPRQTVPTGLLRMAAREAAGIPEWLFDESYDVVGDLAETIAHVLPPARETVDLPLH
                     VWIEQRLLALRGQAPDAVSAALKGYWSELDTPGRFLLTKLIGGSFRVGVARNLVLRAL
                     SEASGVDAKLLAQRVMGYTDKQHAPDAARYALLVDPKQVDTPAESGQPYPFFLAHPLQ
                     APVESLGERADWQVEWKWDGIRAQFIRRGANSWLWSRGEDLLNGRFPELDAMHARLPD
                     TCVLDGEIVVWRDGRVQPFAELQKRIGRKTVTRKILAEQPVAFLAYDLLEIEGVDIRA
                     WPQWQRRERLEQVVAEAGGGPLQLSPRVDAADWSALATLRGESRARGVEGFMLKQREG
                     RYGAGRTKDVGTWWKWKIDPYSVDAVLVYAQGGHGRRANLYTDYTFAVWDGDGEARRL
                     VPFAKAYSGLTDDEMRQVDQRIRQTTIEKFGPVRSVTPTLVVELGFEGIQASSRHKSG
                     IAVRFPRMLRLRWDKPVDEADTLQVLRDFIAESAALRQAD"
     gene            complement(5580..>6017)
                     /locus_tag="VIN75_14910"
     CDS             complement(5580..>6017)
                     /locus_tag="VIN75_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEY8975506.1"
                     /translation="VATMDEAYRQSGVALPPTRNVLEATPDEIRRSLVIAPPSAQGST
                     WTRRFGDSSDAFASGWMLLRGARRRRNVDRGFILSDHADWPGLLEAIAASGAPRVIVT
                     HGYVEPLVRFLAEQGLESGAFKTEYGDDEDAKPVEEAAEAEPT"
BASE COUNT          801 a         2170 c         2234 g          812 t
ORIGIN      
        1 tcgtcgcctg cgggccgccg gacgaggtcg cgcgccgccg ccacggccgc accgcgccgt
       61 acctgcgtgc ggtgctcgac gcgaaggaac ggccatgacc gggctgcacg acctggtgct
      121 ggccctgttc ggggaggacc aggacctgaa cgtgctgcag atggcgatgc gggcgctggc
      181 ggtgttcgtg ctgacgctgg cgatgatccg catcgcgggc cgccgctcgc tgggccagca
      241 tcgtgccttc gacacctgca cgacggtgct cctgggctcc gtgctcagcc gggccgtggt
      301 cggggcgtcg ccgttctggc cgacgatggc cgcgggcgcg gtcttcgtcg tgctccaccg
      361 cctcgtcgcg atggccagcg tgcgctggcc gcggttcgag acgatgatca gcggcgacaa
      421 gcgcgagctc gtgcaagacg gccgtcgtga cgaggcggcg atgcgcaagg cgctcatcac
      481 cgtgcgcgac ctggacgagg cggtgcgcag gaagacgggc gacgagacct cgcaagtgga
      541 gcgcgcgatc ctcgagcgcg atggcggcat cacggtgaag gtcgccgagc cgcccgggtg
      601 agtgcgctgc cttcggggcc cggtcagtcg atgcgcatcc ccggccagac gcgcccgccg
      661 cccaccggcc agcaggtcga gcccgacggc ggcgcctgcg ggctgctgcc ggtgaacgcg
      721 ccgaacgcgg gcagcacgag cacgtcggcg cgatcgtcgg cgatgtggca gaagcaggcg
      781 agccgctcga cgtcgcgcgc cggcccgcgc agcgtcacgg ccgggtgcca gtgtcccgcg
      841 agcaccgcgg cgccggccag ctgccgcgga tgatggcagc cgacgaggcc ggccgcgccc
      901 ggcacgggcc agggctcgtc gacgatctcg aagcgcagcg acggcggcgg gtcgccggcg
      961 tgcgagtcgt ggttgccgcg cacgagcacg cagtgcaggt cggggtgcgc gtcgcgccat
     1021 gcgcgcagcg gcgcgatcgc cgaggcgcgc tgcgcgtgcc gcgagtgcag caggtcgccg
     1081 aggaacacga cgcgtgcggc gccatgcgtc cgcaccagcg tcgcgatgcg atcgaggttg
     1141 tcgcgcgtcg tccccgcggg caccggcagg ccttgcgcgc ggaagctcgc cgacttggcc
     1201 aggtgcaggt ccgcgatgaa cagcgtgcgc gacgccgcat gccaggccgc gcgctcgggc
     1261 agcagcgaca gcgagcggcc ctgcaggacc agcgactgcg tcgtgctcat gtccgcagct
     1321 tggcgcggcg cgtgcgccgc ttcggcttcg gttcgtcgcg gtcggcgatc acggatgcct
     1381 gcgcagcttg ctggagcgca tcgcgcgcga acaccctctt cgcgtcctcg tgcgcggtgc
     1441 ccaccgcgtc gccgccggcg cgaccttcga ggtcggacac catgcgcgcg atgcgctcgg
     1501 cgagcgattc gttggtgagc ttctcgcgga agcgctcgac catcaacggg aacgcgagcg
     1561 gcgagggccg cgcgagcgtc acggcggcca gccgctgccc ctgcatgcgc cgcaacgccg
     1621 cggccagctg gtcgacgtcc agttcctggc gcagcagctc gcgctcggcc tgcgtcagca
     1681 gcaggttgcc ggggtcgtac ctgcggaaca cgtcgaagaa caggctggcc gaggcctgca
     1741 gctggcggtt cgactggcgc tcgccgggat gcgactggaa gatcaggccg gcgatgcggg
     1801 cgatctcccg gaagcgccgg cgcgacatct ccgtcgcatt caggctggcc agcacctcgc
     1861 cgagcagcgc ggccgggtcg tccggcgtcg cgagcaaggc gggcaggcgc tcgccccagg
     1921 cgatctcgcg cgccgacagc agctccaagc cgtagtcgtt gaccgcgatc gagaatgtcg
     1981 cctgctcggg ccgcgcggcg cgccaggcca gcaggctcgc gaggcccagg tgcaccaggc
     2041 ggcccgcgaa cgggtagagg aacaggtgcc agccctcgcg gctcttccag gtctcggcca
     2101 gcagcgtcga cggcgtcggc agcgccgacc accggccctg gatctccagc agcggccgga
     2161 cgcactgcat ctcggggctg tcgaagcggc cgcggtcggc ggcgtcgagc tcgcgcagca
     2221 ccgcgtcggc cagcgtgttg gacagcggca tgcgcccgcc gttccagcgc ggcaccgcgc
     2281 cgcgcccggc gggggcgcgc ttcacgtagg ccgtcatctg gtgcacgcgc accagctcca
     2341 gcaggcggcc gccgaacagg aacgcgtcgc cgggcttcat gcgcgcgatg aagctctcct
     2401 cgacgctgcc caggttgccg ccggtctggt acgcgacgtt gatgctggcg tcgctgacga
     2461 tggtgccgat gttgacgcga tggcgccgcg cgagccgcgc gtcgggcacg cgccagatgc
     2521 ctgcgtcgtc cggcacgacg cgctggtagt ccgggtacgc ggccagcgag gggccgccct
     2581 ggcgcacgaa gtccaggcac cactgccagg ccgcgggcga gaggtcgcgg taggccgtcg
     2641 tcgtgcgcac ctcggcgtac aacgcgtcgg gcatgaagcc gccgccgagc gccacggtca
     2701 ccaggtgctg gacgagcacg tccagcggct cctgcggcga atgccgcgcc tcgacctggc
     2761 cctgctgcgc cgcgtggcgc gcggcggcgg cctccaccag ttccaggctg tgcgtgggca
     2821 ccagcgtcac gcgcgacggc cggcccgggg cgtggcccga gcggccggcg cgctgcagca
     2881 gccgtgcgat gcccttggcc gagccgatct gcagcacgcg gtcgacgggc aggaagtcga
     2941 cgcccaggtc gaggctggac gtgcacacca ccgcgcgcaa cgtgcccgac ttcagccccg
     3001 cctcgaccca ctcgcgcacc tcgcgcgcca gcgagccgtg gtgcagcgcg acgatgccgg
     3061 cccagtcggg gcgcgcgtcc agcagcgcct ggtaccagcg ctcggcctgc gagcgcgtgt
     3121 tggtgaaggc cagcgtcgtg ccgctgtcgt cgatcgcctg gacgacctgc ggcagcatgc
     3181 gcaggcccat gtggccgccc cacgagaagc gctcggcgcg ttcgggcagg agcgtgtcga
     3241 tgacgatggt cttggcgacg tcgccgcgca cgagcgctgg cgcaggcgtc acgcgcgccc
     3301 gcgggcgcac gcccaccagc gtggccagcg cctcctcgag gttgccgagc gtggccgaca
     3361 gcgcccagca catcagcccg ggacggcgct ggcgcagccg cgcgagcgcc agctgcacct
     3421 gcacgccgcg cttgttgccc agcagctcgt gccattcgtc gacgacgacc agctgcacgt
     3481 gtgccagcgc ctggagcgca tcggcgcgcg ccagttgcag cgtcaggctc tcgggcgtcg
     3541 tcaccagcac cgtgggcagc cggcgatcct gcgcgctgcg ctcggcggac gtcgtgtcgc
     3601 cgctgcgcag gccgatcgac cacgtgggtt cgagcgcggc catcggatcg gcgagggcgc
     3661 gcgtggtgtc ggcggccagt gcgcgcatcg gcgtgagcca caggaccgac accggtggtg
     3721 ccgccgcctt cttcgaacgt gacgcggatg cggacgcctg gacacgcaac gcctgcagcg
     3781 cgcccagcca ggccgccagc gtcttgccgg tgccggtgtt ggcgtgcagg agtccgctgc
     3841 ggccttccgc catcgcctgc cagacctcgc gctggaacgc gaacgggtgc cagccgcgcg
     3901 tcgcgagcca ggcctcgacg gcggccgcgt ccgccatcag tccgcctggc gcaacgccgc
     3961 ggattcggcg atgaagtcgc gcagcacctg cagcgtgtcg gcctcgtcga cgggcttgtc
     4021 ccagcgcagg cgcagcatgc gcgggaagcg caccgcgatg cccgacttgt ggcgcgacga
     4081 ggcctggatg ccctcgaagc cgagctcgac gaccagcgtt ggcgtcacgc tgcgcacggg
     4141 gccgaacttc tcgatcgtcg tctggcggat gcgctggtcg acctggcgca tctcgtcgtc
     4201 ggtcaggccc gagtaggcct tggcgaacgg cacgagcctg cgcgcctcgc cgtcgccgtc
     4261 ccacacggcg aaggtgtagt ccgtgtagag gttcgcgcgc cgcccgtggc cgccctgcgc
     4321 atagacgagc actgcgtcga cgctgtacgg gtcgatcttc cacttccacc atgtgcccac
     4381 gtccttggtg cggcctgcgc cgtagcggcc ctcgcgctgc ttgagcatga agccctcgac
     4441 gccgcgggcg cgcgactcgc cgcgcagcgt cgccagcgcg gaccagtcgg cggcgtccac
     4501 gcgcggcgag agctgcagcg ggccgccacc ggcctcggcg acgacctgct cgagccgttc
     4561 gcgacgctgc cactgaggcc aggcgcggat gtcgacgcct tcgatctcca gcaggtcgta
     4621 cgcgaggaag gcgactggct gttcggccag gatcttcctg gtcacggtct tgcgcccgat
     4681 gcgcttctgc agctccgcga acggctgcac gcgtccatcg cgccagacga cgatctcgcc
     4741 gtccagcacg caagtgtcgg gcagccgcgc gtgcatcgcg tcgagctcgg ggaagcggcc
     4801 gttgagcaga tcctcgccgc gcgaccacag ccacgaattg gcgccgcgcc tgatgaactg
     4861 cgcgcggatg ccgtcccact tccactcgac ctgccagtcg gcgcgctcgc ccagcgattc
     4921 gacgggcgcc tgcagcgggt gcgcgaggaa gaacgggtag ggctggcccg attcggccgg
     4981 cgtgtcgacc tgcttcgggt cgaccagcag cgcatagcgc gcggcgtccg gcgcgtgctg
     5041 cttgtcggtg tagcccatca cgcgttgcgc gagcagcttc gcatcgacgc ccgaggcctc
     5101 ggacagcgca cgcagcacga ggttgcgcgc gacgccgacg cgaaagctgc cgccgatcag
     5161 cttggtcagc aggaaacggc cgggggtgtc gagctcgctc cagtagccct tgagcgcggc
     5221 cgagacggcg tcgggcgcct ggccgcgcag cgcgagcagg cgttgctcga tccacacgtg
     5281 aagcggcagg tcgacggtct cgcgcgccgg cggcagcacg tgggcgatgg tctcggccag
     5341 gtcgccgacg acgtcgtagc tttcgtcgaa cagccactcg gggatgccgg cggcctcgcg
     5401 cgcggccatg cgcagcaggc ctgtcggcac cgtctggcgc ggcttgccgc cggccaggaa
     5461 gtacagcgcc caggctgcgt cggcaggctg cgcgcgctgg aagtaggcga gcagcgcctc
     5521 ggtcttcctg cgggtcgacg tggtcgagtc gagcgcggcg aacagcgcgg cgaactcctt
     5581 catgtcggct ccgcttcggc ggcttcctcg acgggcttcg cgtcctcgtc gtcgccgtac
     5641 tcggtcttga acgcgcccga ttccaggccc tgctccgcga ggaagcgcac caacggctcg
     5701 acgtagccgt gcgtgacgat cacgcgcggc gcgccgctgg cggcgatcgc ctccagcagg
     5761 ccgggccagt cggcgtggtc ggacaggatg aagccgcggt cgacgttgcg ccgccggcgc
     5821 gcgccgcgca gcagcatcca gccggaggcg aacgcgtcgc tggaatcacc gaatcgccgc
     5881 gtccacgtgc tgccttgtgc ggacggcggc gcgatgacca ggctgcggcg gatctcgtcg
     5941 ggcgtggcct cgagcacgtt cctggtcggc ggcagcgcga cgccggactg gcggtacgcc
     6001 tcgtccatcg tcgcgac
//
LOCUS       DATCRO010000544         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_591345, whole genome shotgun sequence.
ACCESSION   DATCRO010000544 DATCRO010000000
VERSION     DATCRO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591345"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..997)
                     /locus_tag="VIN75_14915"
     CDS             complement(284..997)
                     /locus_tag="VIN75_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975507.1"
                     /translation="MSNTAARVAIIEDDPPTSDQLKGWIQSARPGLQVDQWFTRDEAE
                     AAIARERYDLVVMDIELGRERHAGVALINAINKQHGTPVLVVSAMPATIYRSIMKALD
                     AWDYLQKTTFEESDFIDTVLEMLRASKDRPAPKTGATTAVDQELSVDPLGQGAVLWRG
                     HRVNLPLTAQRILAALHARRGEVVSYDELFQVVKTGRNRDNVRKHISTIREAFRDIDP
                     QFEGIESVPMRGFRWLARQ"
     gene            1198..2457
                     /locus_tag="VIN75_14920"
     CDS             1198..2457
                     /locus_tag="VIN75_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8975508.1"
                     /translation="MSDNDLHPQYPVEDGPLPPASPLGRLVARLPRVRPLTLALAAAG
                     VVAVWMLASHPPLRGVPAGEVGVRTNRLTGDAGIVRAGSAFVFPGLQELRLVDLREQV
                     YRPTESASASGPAPFQSVEGLSLGVDIATHYAIDPARVTSVARELPPDIGRDVVAPQV
                     QGVIYRVFTRYTVREIFSSRRAEIQQAIVTELRPALAAKGIVLRDVQIGKVDLPPDYR
                     HGMEALLAEELESEKMRFTLELKDKQVKQAALEAEAEKVKREKAAEAAGVEQVIAAKA
                     QEEAMAHVLPFKQKQIQQRQLEAEADKVARIKTAEAAAEARRIEASGEADSRNKLADA
                     EVYRLEKVGKSTSEQMAREGALITRHPLLIQKTMADKLSDKIQVIIAPPPADGGFIGS
                     TLLGRQAAAPATTAKADAGNDTQEAAQ"
     gene            2454..>3610
                     /locus_tag="VIN75_14925"
     CDS             2454..>3610
                     /locus_tag="VIN75_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975509.1"
                     /translation="MRVRTSRGSGQIGYRPTRRRHDFGHPHAQGSPLRTLAIWLLASL
                     AAIACIVYPPTAHAASPAPAAALAARSVGVAAKTPAPVAATTTPESSRYALVTQDAVP
                     LRAGARSGGSPLALLTQGELLEVRGERLDYLQVWDLARERGGFVRASAVRALSLASAE
                     APELLALVRFLRDRPGSESLGLGVAAAYLKAAPAGSIHADVFDAMGTMAERLAWRANV
                     AAANAANVPVRPGEPAVAAQLEVAADLGVKFTSLERDGHVWLCYDGDAFRHVLALQPS
                     PEQSARAALALTRHDCVDDMTPPLQRWQVDQARAALLARVPVAGLPPYVRNRIRMRQA
                     GVWSAIAYEDTRRLAVESGAAPGGLPAVGANDVLVAGNAALEALAGVDRSEL"
BASE COUNT          499 a         1325 c         1313 g          473 t
ORIGIN      
        1 gcggtagctg cggtggctca gctccttctc gtcctgcagc accgacagcg cgatcgccac
       61 ggtggccagc gccagcagca ggaacacgcc gcggaagaac agccgcaggc ggaagaacgc
      121 gatgccgggc cagcgccagg cggcaaggcg gtcacgccac ctcatgcgtc gtcatcctgc
      181 gcgcagtcgc aggatccatc cccgccatgc ggcctccggg cggcgttgcc gcgcgcgtcg
      241 atcccgcgac tgcgcgcagg atgacggaca tgggtgtcgg agctcactgc ctggcgagcc
      301 accggaaccc gcgcatcggc acgctctcga tgccttcgaa ctgcggatcg atgtcgcgga
      361 acgcttcgcg gatcgtgctg atgtgcttgc gcacgttgtc gcggttgcgt cccgtcttca
      421 cgacctggaa cagctcgtcg tacgacacca cctcgccgcg gcgcgcatgc agggccgcga
      481 ggatgcgctg cgcggtcagc ggcaggttga cccggtggcc gcgccacagc acggcgccct
      541 ggccgagcgg gtcgacgctg agctcctggt cgacggccgt cgtcgcgccg gtcttcgggg
      601 cggggcggtc cttggaggcg cgcagcatct cgagcacggt gtcgatgaag tcggactcct
      661 cgaaggtggt cttctgcagg tagtcccagg cgtcgagcgc cttcatgatg ctgcggtaga
      721 tcgtggccgg catcgccgag accaccagca ccggcgtgcc gtgctgcttg ttgatcgcat
      781 tgatcagcgc gacgccggca tggcgctcgc gcccgagctc gatgtccatc accaccaggt
      841 cgtagcgctc gcgggcgatc gcggcctcgg cctcgtcgcg cgtgaaccac tggtcgacct
      901 gcagccccgg gcgcgccgac tggatccagc ccttgagctg gtcgctcgtg ggcgggtcgt
      961 cttcgatgat cgcgacgcgg gcggcggtgt tcgacacggt gggcctttcg gtggacggac
     1021 gcgcgttgcc gcatcctgcc gcccgattat ccggtcgcct cgcagggggc gccgtgaggg
     1081 tttgctccgc gccgcggaag gcaccggcgg ccggcgtgaa cgcagcaccc ccgcggcgcc
     1141 gttgaacgct cggcgcgggc ctcgaagaat ggcctcacac accgaggaga gacccccatg
     1201 agcgacaacg acctgcatcc ccaatacccc gtcgaggacg gcccgctgcc gccggccagt
     1261 cccctcggac ggctcgtcgc ccgcctgccg cgcgtgcgtc cgctgacgct ggcgctcgcg
     1321 gccgccggcg tcgtcgcggt ctggatgctg gccagtcacc cgccgctgcg cggcgtgccg
     1381 gccggcgagg tcggcgtgcg caccaaccgg ctgactggcg acgccggcat cgtgcgcgcc
     1441 ggctccgcct tcgtcttccc gggcctgcag gagctgcgcc tggtcgacct gcgcgagcag
     1501 gtctatcgcc cgacggaaag cgcgtcggcg agcggcccgg cgccgttcca gtcggtcgag
     1561 gggctgtcgc tgggcgtgga catcgccacg cactacgcga tcgatccggc gcgcgtgacc
     1621 agcgtggcgc gcgagctgcc gcccgacatc ggccgcgacg tcgtcgcgcc acaggtgcag
     1681 ggcgtcatct accgcgtctt cacgcgctac acggtgcgcg agatcttctc gtccaggcgc
     1741 gccgagatcc agcaggcgat cgtcaccgaa ctgcgccccg cgctggcggc caagggcatc
     1801 gtgctgcgcg acgtgcagat cggcaaggtc gacctgccgc cggactacag gcacggcatg
     1861 gaggcgctgc tcgccgagga gctcgagagc gagaagatgc gcttcacgct ggaactcaag
     1921 gacaagcagg tcaagcaggc cgcgctggag gccgaggccg agaaggtcaa gcgcgagaag
     1981 gccgccgagg ccgccggcgt ggagcaggtg atcgcggcca aggcgcagga ggaggcgatg
     2041 gcccacgtgc tgccgttcaa gcagaagcag atccagcagc gccagctcga ggccgaggcc
     2101 gacaaggtcg cccgcatcaa gaccgccgag gccgcggccg aggcacgccg catcgaggcg
     2161 tcgggcgagg cggactcgcg caacaagctg gccgacgccg aggtctaccg cctggagaag
     2221 gtcggcaagt ccaccagcga gcagatggcg cgcgaggggg cgctgatcac gcgccacccg
     2281 ctgctgatcc agaagacgat ggccgacaag ctgtccgaca agatccaggt gatcatcgcg
     2341 cccccgcccg cggacggtgg cttcatcggc tcgacgctgc tgggcaggca ggccgcggcg
     2401 ccggcgacca cggccaaggc cgacgccggc aacgacacgc aggaggccgc gcaatgagag
     2461 tccgcacctc gcgcggcagc ggccagatcg gctaccgccc gacgcgccgc cgccacgact
     2521 tcggccaccc gcacgcccag ggcagcccgc tgcgcacgct ggcgatctgg ttgctggcga
     2581 gcctggccgc catcgcctgc atcgtctatc cgccgaccgc gcatgccgcg tccccggcgc
     2641 ccgcggcggc gctggccgcg cgcagcgtcg gcgtggccgc caagacgccg gcgccggtcg
     2701 ccgcgacgac cacgccggag tcgtcgcgct acgcgctcgt cacgcaggac gccgtcccgc
     2761 tgcgcgccgg cgccaggagc ggcggcagcc cgctggcgct gctgacccag ggcgaactgc
     2821 tggaggtgcg cggcgagcgc ctggactacc tgcaggtctg ggacctcgcc cgcgaacgcg
     2881 gcggcttcgt gcgcgcgagc gccgtgcgcg cgctctcgct cgcgtccgcg gaggcgcccg
     2941 agttgctggc gctggtgcgc ttcctgcgcg atcgccccgg gtccgaatcg ctgggcctgg
     3001 gcgtggccgc cgcctacctg aaagccgcgc cggccggctc gatccacgcc gacgtgttcg
     3061 acgcgatggg cacgatggcc gaacgcctgg cctggcgcgc caacgtggcc gccgccaatg
     3121 ccgccaacgt gccggtgcgc ccgggcgagc ccgccgtcgc ggcgcaactg gaggtggcgg
     3181 ccgacctcgg cgtgaagttc acgagcctgg agcgcgacgg ccacgtctgg ctctgctatg
     3241 acggcgatgc gttccgccac gtgctggcgc tgcagccgtc gcccgagcag tcggctcgcg
     3301 ccgcgctggc cttgacgcgc cacgactgcg tcgacgacat gaccccgccg ctgcagcgct
     3361 ggcaggtcga ccaggcgcgc gcggccctgc tggcgcgggt gccggtcgcc gggctgccgc
     3421 cgtacgtgcg caaccgcatc cgcatgcgcc aggccggggt ctggtcggcg atcgcgtacg
     3481 aggacacccg ccgcctcgcc gtcgaatccg gcgccgcgcc cggcggcctg ccggcggtcg
     3541 gcgccaacga cgtgctcgtg gccggcaacg cggcgctgga ggcgctggcc ggcgtcgacc
     3601 ggtccgagct
//
LOCUS       DATCRO010000545         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_354217, whole genome shotgun sequence.
ACCESSION   DATCRO010000545 DATCRO010000000
VERSION     DATCRO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354217"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /gene="metG"
                     /locus_tag="VIN75_14930"
     CDS             complement(<1..996)
                     /gene="metG"
                     /locus_tag="VIN75_14930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8975510.1"
                     /translation="MTRKLFVTTALPYANAPFHIGHMMEYIQADIWVRFQRMRGATVH
                     FVGADDAHGAPIMIAAEKQGKTPQAFVAEVAAGRAKYLDGFHIAFDNWSSTDSPENHA
                     LSREIYLALRKNELIDVKTIDQFFDPVKSMFLPDRFIKGECPRCGTKDQYGDACENCG
                     AVYSPTELKNPYSALSGATPVMKSSEHYFFRLSDPRCLEFLQKWTTDTGRLQPEVLNK
                     IKEWFQKDPETGAGGLGDWDISRDAPYFGIEIPDAPGKYFYVWLDAPIGYLASLKNYF
                     GKKGLDYDAFFADPDVQQVHFIGKDITYFHTLFWPAMLHFSGRKTPDHVFVHGFIT"
     gene            1259..2362
                     /locus_tag="VIN75_14935"
     CDS             1259..2362
                     /locus_tag="VIN75_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975511.1"
                     /translation="MNLLWNPAARRLRAGLATALALLGLAPASRATDIAHAAIPSVVA
                     DNKISIGSHAFRLPPGQWVLVQGQTFTTASASNRDAEIFRGVVVLLDKNQFRVAIDVS
                     LPVRDSPQMTPWGEDACRANGGALHATPMTKFNGEQCLAIFGHPDLLRTLRRNNPQVA
                     QWMLDQHVVGLGSAVEIVYTSRQDATFGRMHVFFTAPYFDSDEAMTRWSVALEDSVKR
                     EFASKSFTAAVLPALPVLPASIQANDSASDMVRPAAQVEAAAAAAREEWMACLRTQAA
                     RLDDRRTPANEVGSALYFNCREQELKGVTANIPPARLQVMSTSEINELFENAHRQVVD
                     SGVATNIVLEHRASRAPDRPASPADKPAKSVKR"
     gene            complement(2352..3248)
                     /locus_tag="VIN75_14940"
     CDS             complement(2352..3248)
                     /locus_tag="VIN75_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8975512.1"
                     /translation="MKRLFARSPLVVMLAAPLLAHAVDITAGTSEYITFRDCIAGVTA
                     CDSISPIVSGQYGGNPGAYSSAATSNHAGYGSASGSVSFSGVIGAPVLHASASADPGK
                     RANTNSVALQSWTYTGSTPTTRTFGGTLTYNQALTGTYPDTGATGVYATIDAFTLTAA
                     SIDAGIDAASNFNALFGEPWTADPSTGYVDLAQNTFSDDTSQAGATGNLGVTVSLTPG
                     ETVWLYVLLQTPAVNGSVVDASHTLVTGWSDATDLVPAVTSAPVPEPSSWALIGCGLA
                     AVAVLRRRHDRTRRTRSRLLSA"
     gene            3456..4859
                     /locus_tag="VIN75_14945"
     CDS             3456..4859
                     /locus_tag="VIN75_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8975513.1"
                     /translation="MSVDSPAATALRLPAADGLAGRQHVLALVAILLSVAMATLDTAI
                     ANTALPTIASSLRVSEAQVIWVVNAYQLAMVAVLLPFAASGEILGHRRVFAAGTLLFT
                     VASLVCGLAGSLTALVAARILQGLGGAAIMSVNMTLIRVVFPASRLGRGAGMNALVVG
                     IAFTVGPTVASAVLAVASWHWLFLINVPVGIVAVLFAARALPHTERHDHGFDRVAALL
                     CAGLFGCAVLGFESLSHGGAWLPVAAAWAVAGACGALLWRRQAGHPAPMLPVDLFRSP
                     AFSLSALTAVCAFSAQSFGFVALPFLLQSNLGYSAVATGLLMTPWPAVVALMAPIAGP
                     LADRRPPGLLGGLGLVIFGVGLAALALLPAHAAVADIAWRTAVCGAGFGFFQAPNLKA
                     LMGSVPARRSGSASGVVATSRLIGQTLGAGLVALCFHLSAAVAPTLALWLGCAFALAG
                     STASVLRLLPAARVVPR"
     gene            complement(4867..>5317)
                     /locus_tag="VIN75_14950"
     CDS             complement(4867..>5317)
                     /locus_tag="VIN75_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975514.1"
                     /translation="HEKPGGGGYPAGVTEVGEMASALRTVDVYTAKLAGRSGRDSLPP
                     PRAARDLLVVERGNPFALALITEFGVHPPGSLVRLYSGEIAVVLRRTADSGAPQVAVL
                     AGRRGEPLAEPMLLEASGRDRGIAGPADPHGLRVRISPERLYALPSP"
BASE COUNT          673 a         1838 c         1986 g          820 t
ORIGIN      
        1 cgtgatgaag ccgtgcacga acacgtggtc gggcgtcttg cggcccgaga agtgcagcat
       61 cgccggccag aacagcgtgt ggaagtacgt gatgtccttg ccgatgaagt gcacctgctg
      121 cacgtcgggg tcggcgaaga aggcgtcgta gtccaagccc ttcttgccga agtagttctt
      181 gagcgaggcg aggtagccga tcggcgcgtc cagccagaca tagaagtact tgcccggcgc
      241 gtcggggatc tcgatgccga agtagggcgc gtcgcggctg atgtcccagt cgcccaggcc
      301 gccggcgccg gtctcgggat ccttctggaa ccactccttg atcttgttca ggacctccgg
      361 ctgcaggcgg ccggtgtcgg tcgtccactt ctgcaggaac tccaggcagc gcgggtcgga
      421 cagccggaag aagtagtgct cgctggactt catcaccggc gtcgcgccgc tgagcgccga
      481 gtacgggttc ttcagctcgg tggggctgta gacggcgccg cagttctcgc aggcgtcgcc
      541 gtactggtcc ttcgtgccgc agcgcgggca ttcgcccttg atgaagcggt ccggcaggaa
      601 catggacttc accgggtcga agaactggtc gatcgtcttg acgtcgatca gctcgttctt
      661 gcgcagggcg aggtagatct ccctggacag cgcgtggttc tcgggcgagt ccgtggagct
      721 ccagttgtcg aaggcgatgt ggaagccgtc gaggtacttc gcgcggccgg ccgccacctc
      781 ggcgacgaag gcctgcggcg tcttgccctg cttctcggcg gcgatcatga tcggcgcgcc
      841 gtgggcgtcg tcggcgccca cgaagtgcac cgtggcgccc ctcatgcgct ggaaccgcac
      901 ccagatgtcg gcctggatgt actccatcat gtgcccgatg tggaagggcg cattggcgta
      961 gggcagggcg gtggtgacga agagtttgcg ggtcatggcg gcgatggtcg tccccgggcg
     1021 cgcgaggggc gcggcggcgg cgcgaaagcg gaacccacga ttctaagggc gccgggacgg
     1081 atcggctggc cagggcccat ggccgggcag ccgcggcgcg cccgcgccat ccccaattcg
     1141 ttggggccgg cgcccggcaa atgtcacacc acggcgagct tgcggcaggc ttgctcgacg
     1201 ggcgatgacg taaagttcgg aacgctttcg tcggcccctc ccacgccaag gatccccctt
     1261 gaacctgctc tggaaccccg ccgcgcgccg cctccgggcc ggcctcgcga ccgccctggc
     1321 cctgctgggc ctcgcgcccg cgagccgcgc gaccgacatc gcccatgccg cgatcccgtc
     1381 cgtcgtggcc gacaacaaga tctcgatcgg ctcgcacgcc ttcaggctgc cgccgggcca
     1441 gtgggtgctg gtgcagggcc agaccttcac cacggccagc gccagcaacc gcgacgccga
     1501 gatcttccgc ggcgtggtcg tgctcctgga caagaaccag ttccgcgtcg cgatcgacgt
     1561 ctcgctgccg gtgcgcgatt cgccgcagat gacgccctgg ggcgaggatg cctgccgtgc
     1621 caacggcggc gcgctgcacg ccacgccgat gaccaagttc aacggcgaac agtgcctggc
     1681 catcttcggc catcctgacc tgctgcgcac cctgcgccgc aacaacccgc aggtcgcgca
     1741 gtggatgctc gaccagcacg tcgtcggcct cggcagcgcc gtcgagatcg tctacacgag
     1801 ccgccaggac gccaccttcg gccgcatgca cgtgttcttc accgcgccgt acttcgacag
     1861 cgacgaggcc atgaccaggt ggtcggtggc gctggaggac tcggtcaagc gcgagttcgc
     1921 gagcaagtcc ttcaccgcgg cagtgctgcc ggcgctgccg gtgctgcccg cgtcgatcca
     1981 ggccaacgac tcggcctccg acatggtgcg ccccgcggcg caggtggagg cggccgcggc
     2041 cgctgcgcgc gaggaatgga tggcctgtct gcgcacgcag gccgcgcggc tggacgaccg
     2101 ccgcacgccg gccaacgagg tcgggtcggc gctctacttc aactgccgcg agcaggagtt
     2161 gaagggcgtg acagccaaca tcccgcccgc gcggctgcag gtgatgtcga cgtccgagat
     2221 caacgagctg ttcgagaacg cgcaccgcca ggtggtcgac tcgggcgtgg ccaccaacat
     2281 cgtgctggag caccgcgcgt cgcgcgcgcc cgaccgtccg gcgtcgccgg cggacaagcc
     2341 ggccaagtcg gtcaagcgct gagaagccgg gaacgagtcc ggcgcgtccg gtcgtgccgc
     2401 cggcgcagca cggcgacggc cgcgaggccg cagccgatca gcgcccatga cgacggctcg
     2461 ggcacgggcg cgctcgtgac cgcgggcacg aggtcggtcg cgtcgctcca tccggtgacg
     2521 agcgtgtgcg aggcgtcgac gacgctgccg ttgacggccg gcgtctgcag cagcacgtac
     2581 agccacaccg tctcgccggg cgtcaggctc accgtgacgc ccaggttgcc ggtggcgccc
     2641 gcctgcgagg tgtcgtccga gaacgtgttc tgcgccaggt cgacgtagcc ggtcgacgga
     2701 tcggccgtcc acggctcgcc gaacagcgcg ttgaagttcg aggccgcgtc gatgccggcg
     2761 tcgatgctgg cggccgtcag cgtgaaggcg tcgatcgtcg cgtagacgcc ggtggcgccg
     2821 gtgtcgggat aggtgccggt gagggcctgg ttgtaggtca gggtgccgcc gaaggtgcgc
     2881 gtggtgggcg tgctgccggt gtaggtccag ctttgcagcg ccaccgaatt ggtgttggcg
     2941 cgcttgcccg ggtcggcgga cgcgctcgcg tgcagcaccg gcgcgccgat cacgcccgag
     3001 aagctgacgc tgcccgaggc cgagccgtac ccggcgtggt tgctcgtcgc cgccgaggag
     3061 tacgcgccgg ggttgccgcc gtactggccg ctgacgatgg gcgagatgct gtcgcaggcg
     3121 gtgacgccgg cgatgcagtc gcggaaggtg atgtactcgg aggtgccggc cgtgatgtcg
     3181 accgcgtgcg caagcagcgg cgcggcaagc atcacgacga gggggctgcg ggcgaacagg
     3241 cgcttcatgg gggctccgtg gaatcgttgg cgggcggcgc cacggtcggg caccgagcgc
     3301 gatcctcacg gatgccgggc cgggcaccca tccctcgacc gaggtatgtc gcgcgaacca
     3361 cgatgtgaac cggatccggg cgccgcaaag gcccgccgcg ccgctgcggc atcgccgcgg
     3421 tgatgaaatg cgaggactcc ctcatctttg ccgcgatgtc cgtcgactcc cccgccgcca
     3481 cggcgctccg cctccccgcc gcagacggcc tggccggccg ccagcacgtg ctcgcgctcg
     3541 tcgcgatcct gctgtcggtg gcgatggcga cgctggacac cgccatcgcc aacaccgcgc
     3601 tgccgacgat cgcgtccagc ctgcgcgtga gcgaggcgca ggtgatctgg gtcgtcaacg
     3661 cctaccagct ggcgatggtg gcggtgctgc tgccgttcgc ggcgtcgggg gagatcctgg
     3721 gccaccggcg cgtgttcgcg gccggcacgc tgctgttcac ggtcgcctcg ctcgtgtgcg
     3781 ggctggccgg ctcgctgacg gcgctggtcg ccgcgcgcat cctgcagggg ctgggcggcg
     3841 ctgccatcat gagcgtcaac atgacgctga tccgggtcgt gttcccggcc agccggctgg
     3901 ggcgcggggc gggcatgaac gcgctggtcg tgggcatcgc gttcacggtg gggccgacgg
     3961 tggcgtcggc ggtgctggcg gtggcctcgt ggcactggct gttcctgatc aacgtgccgg
     4021 tcggcatcgt cgccgtgctg ttcgcggcgc gcgcgctgcc gcacaccgag cgccacgacc
     4081 acggcttcga ccgcgtcgcg gcgctgctgt gcgcgggcct gttcggctgc gcggtgctgg
     4141 gcttcgagtc gctgtcgcac ggcggcgcct ggctgccggt ggccgcggcg tgggcggtgg
     4201 ccggcgcctg cggggcgctg ctgtggcggc gccaggccgg ccatccggcg ccgatgctgc
     4261 cggtcgacct gttccgctcg ccggcgttct cgctgtccgc tctcacggcg gtctgcgcgt
     4321 tctcggcgca gagcttcggc ttcgtggcgt tgccgttcct gctgcagtcc aacctcggct
     4381 acagcgcggt cgccacgggc ctgctgatga cgccgtggcc ggcggtggtg gccctgatgg
     4441 cgccgatcgc cgggccgctg gccgaccgcc gtccgccagg cctgctgggc ggcctcgggc
     4501 tcgtgatctt cggcgtgggt ctggccgcgc tggcgctgct gccggcgcat gccgcggtgg
     4561 ccgacatcgc ctggcgcacc gcggtctgcg gcgccggctt cggcttcttc caggcgccca
     4621 acctcaaggc cttgatgggc agcgtgccgg cgcgccgcag cggcagcgcc agcggcgtgg
     4681 tggccacgtc gcgcctgatc gggcagacgc tgggcgccgg actcgtggcg ctgtgcttcc
     4741 acctgagcgc cgccgtcgcg ccgacgctgg cgctgtggct gggctgcgcg ttcgcgctgg
     4801 cgggcagcac ggcgagcgtg ctgcgcctgt tgccggcggc gcgcgtcgtg ccgcgctgac
     4861 ccggcgtcag ggcgacggca acgcgtacag ccgctcgggg ctgatgcgca cgcgcaggcc
     4921 gtgcgggtcg gcggggccgg cgatgccgcg gtcgcggccg ctggcctcca gcagcatggg
     4981 ctcggccagc ggctcgccgc gccgtccggc gagcacggcg acctgcggtg cgccggagtc
     5041 ggcggtgcgc cgcaggacga ccgcgatctc gccgctgtac aggcgcacga ggctgccggg
     5101 cggatgcacg ccgaactcgg tgatcagcgc cagcgcgaac ggattgccgc gttcgacgac
     5161 gagcaggtcg cgcgccgcgc gcgggggcgg caggctgtcg cggccgctgc gcccggccag
     5221 cttggcggtg tagacgtcca ccgtgcgcag cgcgctggcc atctcgccca cctcggtgac
     5281 cccggccgga tagccgccgc cgccgggctt ctcgtgg
//
LOCUS       DATCRO010000546         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_186301, whole genome shotgun sequence.
ACCESSION   DATCRO010000546 DATCRO010000000
VERSION     DATCRO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186301"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VIN75_14955"
     CDS             complement(<1..625)
                     /locus_tag="VIN75_14955"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8975515.1"
                     /translation="MTDDVLALLAGGPRLPLPLLERLFDALPDVVFFAKDAEGRYTHA
                     NQTLVDRLRLTKRAQLVGRRADELFPGGLGDRYRGQDDAVLRGGPEIVGQLELHLYPN
                     RAPGWCLTHKLAWREPTPGGGRRRERIVGLVGLSRDLATPGLSHATPPATYARIAHVV
                     ERLQRDYAEPLQIAALAREAGLSIAQLERHVTQLYRVTPRQLLARARL"
     gene            706..1659
                     /locus_tag="VIN75_14960"
     CDS             706..1659
                     /locus_tag="VIN75_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY8975516.1"
                     /translation="MHKIVVIDSHTAGEPTRVVLEGGPALAGATLAEQRAEFARHHDA
                     FRAAVACEPRGSDTMVGALLVQPRAPGCVAGMIFFNNVGLLGMCGHGTIGVVATLAHL
                     GRIGPGRHRLDTPVGPVETELHADGHVTVFNVPSWRHRAGVTLDVPGVGPVAGDVAWG
                     GNWFFLVDATQFEPAPDLALAHVEALTDLTWRIRGALAAQGITGADGAEIDHIELFAP
                     SPTADARNFVLCPGKAYDRSPCGTGTSAKLACLAADGHLAAGVAWVQESVIGSRFEGR
                     WTPVDGRPGHILPAIRGRAWITAEATLLRAEDDPFRDGIRA"
     gene            1718..2848
                     /locus_tag="VIN75_14965"
     CDS             1718..2848
                     /locus_tag="VIN75_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8975517.1"
                     /translation="MTRPVDVAIVGGGVVGCACAHAAVQAGLRVAVYDAGRIGAGATA
                     AGMGHLVTIDDDPAEFALARLSMTLWRAWSGWAAIERRTTGTLWLAETDRQRDAAHAK
                     LARLRAAGLSAEWLEPTVLASAEPRLARDLAGALRVAGDEVAYGPELAARLAADVRAA
                     GGAIEEMCPVAALRDDGLQLSSGGRVSAGHVLLAAGCDSVRLWPGDAPLPLRPRKGQL
                     AITDRYPGYVRHALVELGYIDAAHGDADESVAFNVQPRASGQVLLGSTRSYRDTSPDV
                     DAALLARMVRRAQRFLPGIGALQMLRCWAGFRPAIADGRPAIGRIPGARRDNQWVATG
                     HEGLGLTTATGTAGVWLDLLLGRAPAIDPAPYAPDRFVAVAA"
BASE COUNT          350 a         1112 c         1120 g          365 t
ORIGIN      
        1 cgagccgcgc gcgcgccagc agctggcgcg gcgtcacgcg gtacagctgc gtgacgtgac
       61 gctccagctg cgcgatcgac agccccgcct cgcgcgccag cgcggcgatc tgcagcggct
      121 ccgcgtagtc gcgctgcagc cgctcgacga catgcgcgat gcgcgcgtac gtcgcgggcg
      181 gcgtcgcgtg gctcaggccc ggcgtggcca ggtcgcgcga caggcccacc aggccgacga
      241 tgcgctcgcg ccggcggccg ccgccaggcg tcggctcgcg ccatgccagc ttgtgcgtga
      301 ggcaccagcc gggcgcgcgg ttcgggtaca ggtgcagctc cagctggccg acgatctcgg
      361 gcccgccgcg cagcacggcg tcgtcctgcc cgcggtaacg gtcgcccagg ccgccgggga
      421 acagctcgtc ggcgcgccgg cccaccagct gtgcgcgctt cgtcaggcgc aggcggtcga
      481 ccagcgtctg gttcgcgtgc gtgtagcgcc cctcggcgtc cttggcgaag aagaccacgt
      541 cgggcagggc gtcgaacagg cgttccagca ggggcagcgg caggcgcggg ccgccggcaa
      601 gcagggcgag gacgtcgtcg gtcatgcttg cattgtgctg aaatcgtcat ggcgatgcac
      661 gcgacggatc aagccgcgcg ggcccgccgg ttccagaatc gcggcatgca caagatcgtc
      721 gtcatcgatt cccacaccgc cggcgagccg acgcgcgtcg tcctcgaggg cgggcccgcg
      781 ctggccggcg cgacgctggc cgagcagcgc gccgagttcg cgcgccacca cgacgccttc
      841 cgcgccgccg tcgcctgcga gccgcgcggc agcgacacga tggtcggcgc gctgctggtg
      901 caaccgcgcg cgccgggctg cgtggccggc atgatcttct tcaacaacgt cggcctgctg
      961 ggcatgtgcg gccacggcac gatcggcgtg gtcgccacgc tggcgcacct gggccgcatc
     1021 gggccgggcc gccatcgcct ggacaccccc gtcggccccg tcgagaccga gctccacgcc
     1081 gacggccatg tcaccgtgtt caacgtgccg agctggcgcc accgcgccgg cgtcacgctg
     1141 gacgtgcccg gcgtcggtcc cgtcgccggc gacgtcgcgt ggggcggcaa ctggttcttc
     1201 ctcgtcgacg cgacgcagtt cgagccggcg cccgacctcg cgctggcgca cgtcgaggcg
     1261 ctgaccgacc tgacgtggcg catccgcggc gcgctcgccg cgcaaggcat caccggcgcg
     1321 gacggcgccg agatcgatca catcgagctg ttcgccccgt cgcccacggc ggacgcgcgc
     1381 aacttcgtgc tgtgccccgg caaggcctac gaccgctcgc cgtgcggcac cggcaccagc
     1441 gccaagctcg cctgcctggc cgcggacggc catctcgcgg cgggcgtcgc gtgggtgcag
     1501 gaaagcgtga tcggcagccg cttcgagggc cgctggacgc cggtcgacgg ccggcccggc
     1561 cacatcctgc cggccatccg cggccgcgcc tggatcacgg ccgaggcgac gctgttgcgt
     1621 gccgaggacg atcccttccg cgacggcatc cgcgcatgag tccgcacggc cgtccgaagg
     1681 gcgaatcgac cgcggcgcgc tgcgcggagc ttgcctgatg acccgtccgg tcgacgtcgc
     1741 gatcgtcggc ggcggcgtgg tcggctgcgc ctgcgcgcac gcggccgtgc aggccgggct
     1801 gcgcgtggcg gtctacgacg ccggacgcat cggcgccggc gcgaccgcgg caggcatggg
     1861 ccatctcgtg acgatcgacg acgacccggc cgaattcgcg ctggcgcgcc tgtcgatgac
     1921 gctgtggcgc gcctggagcg gctgggccgc gatcgagcgc cgcaccacgg gcacgctgtg
     1981 gctggccgag accgacaggc agcgcgacgc cgcccacgcg aagctggcgc gcctgcgcgc
     2041 cgcgggactg tcggccgagt ggctggagcc cacggtgctg gcgtccgccg agccgcgcct
     2101 ggcgcgcgac ctcgcgggcg cgctgcgcgt ggccggcgac gaggtggcgt acgggccaga
     2161 gctcgcggcg cggctcgcgg ccgacgtgcg cgccgccggc ggcgccatcg aggagatgtg
     2221 ccccgtcgcc gcgctgcgcg acgacggcct gcaactgtcc agcggcggcc gcgtcagcgc
     2281 gggccacgtg ctgctggccg ccggctgcga cagcgtccgc ctgtggcccg gcgatgcgcc
     2341 gttgccgctg cgtccgcgca aggggcagct ggccatcacc gaccgctatc cgggctacgt
     2401 gcgccacgcg ctggtggaac tgggctacat cgatgccgcc cacggggacg ccgacgagtc
     2461 ggtcgccttc aacgtgcagc cgcgcgcatc gggccaggtg ctgctgggtt cgacgcgcag
     2521 ctatcgcgac acgagccccg acgtcgacgc cgcgctgctg gcgcgcatgg tgcgacgcgc
     2581 gcagcgcttc ctgcccggca tcggcgcgct gcagatgctg cgctgctggg ccggcttccg
     2641 gcccgcgatc gcggacgggc ggccggcgat cggccgcatc ccgggcgcgc ggcgcgacaa
     2701 ccagtgggtc gccacgggcc acgagggcct gggcctgacc acggccaccg gcacggccgg
     2761 cgtgtggctg gacctgctgc tgggccgcgc gcccgccatc gatccggcgc cgtacgcgcc
     2821 cgatcgcttc gtggcggtgg cggcatgacg gacgcgatca cggtcaccgt cgacgggcgc
     2881 gcactggacg tgcggcccgg cacgatggtc gcggcggccg tcgaactggc cgcgcccggg
     2941 cggggcg
//
LOCUS       DATCRO010000547         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_861346, whole genome shotgun sequence.
ACCESSION   DATCRO010000547 DATCRO010000000
VERSION     DATCRO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861346"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1590)
                     /locus_tag="VIN75_14970"
     CDS             complement(22..1590)
                     /locus_tag="VIN75_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8975518.1"
                     /translation="MSAPAAAAKPPAPLTGSALVLGTLSLSLATFMNVLDSSIANVSL
                     PSISGDLGVSISQGTWVITSFGVANAISVPLTGWLTQRFGAVRLFTLSVILFVIASTL
                     CGLAPSIEMLIAFRVLQGFVAGPMIPLSQTLLLSTYPPQKAGTALALWGVTTLVAPVA
                     GPLLGGYITDNWTWPWIFYINIPVGIFAAFITWSIYHKRETPTVKRPIDGVGLGMLVL
                     WVGCLQVMLDKGKELDWFNSPTIVTLAVIVVIAFAVFVAWELTDEHPVVDIRLFAGRN
                     FAFGAATLSIAYGLFFGNVVLLPLWLQQYMGYTATSAGMALAPVGILAIVLTPIVGRN
                     VAKWDPRWMATGAFILFALVLWMRSKFTSDTDFGTILLPTLIQGGAMAFFFIPLTTLT
                     LAGLPPSRIPAAAGLSNFVRITMGAMGTSIATTLWDNRATMHHEHLVEQMAMNGTALD
                     NATTALTQAGLPTAGAYASINRLVDVQAFTKAADDIFIASAVTFLFLIFTIWFTKRPP
                     RIAGGPSVDAGGAH"
     gene            complement(1656..3005)
                     /locus_tag="VIN75_14975"
     CDS             complement(1656..3005)
                     /locus_tag="VIN75_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8975519.1"
                     /translation="MNAPANNNAAPDTTAPAGNPKRRTALTAIAVAVLVIGGGYGIYY
                     WMTGRHSESTDNAYVQANVVQITAQAGGTVTAINADDTDYVKAGQVLVKLDPADARVA
                     LDQAEAALAQTVRETRILFANNSTLQAQIDARAADATRAAADLAKAQDDVNRRGPLVQ
                     SGAVGKEEFEHAQQQLQSARSAYTAAKSALDAAKEQLASNQSQTEGTSVAQHPNVQRA
                     AAKVREAWLAVQRTDVPAPVSGTVARRSVQVGQRVQAGVPLMSVVTLNNAWVDANFKE
                     GQLERLRIGQPATLEADVYGKHVVFHGKVEGLGAGTGAAFSLLPAQNATGNWIKVVQR
                     VPVRISLDPAEVAKNPLRVGLSMNVKVDVADESGRSVADTPRTQGAVASTTVFDSLQR
                     DADTEVARIVAANGGGRIAPTATAQAASSSASGPVAAASGAEKIASAASHRHHHKQS"
     gene            complement(3012..4637)
                     /locus_tag="VIN75_14980"
     CDS             complement(3012..4637)
                     /locus_tag="VIN75_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8975520.1"
                     /translation="MPSATRQQECPRRAGASPKTGETQSINSRKPAKMTHRGLELATP
                     RVLSASTFALAVASAAVLVLAGCASTGHQSEPVAQRDAQALGLSASQQTTAVDAQWWH
                     QFGDAQLDALVAKALEGSPSLGSAQARLTRAQAVVEAARGAELPAVAFDASVKRELLS
                     RNGLFPPPYGGSIFNQSNATFGLSWDADVFGRQKHDLESALGSARAARADADVAAQQI
                     ATQVVRTYFSLARLESQRAVAQRTYDQRKQMLSLITQRVSAGLDTVVEQKQGEGALPD
                     TLAQIESLDEQIDLARHALAALTAQPMDATARLAPTLDQLHVSPQPPVLGADLIARRP
                     DVAAALARVEASKEAVASQKTRFYPDINFSAGFGLDSINLGKFLELPSKTWSFGPALH
                     LPIFEGGQLRAQLKGKGAERDAAIQAYNQQVIDAVREAADAATSAASIGRQLALQRQA
                     LEASTASYDFSQKRYGQGLGNALIVLNAETQMLAERRLEVDLEYRALDVQAQLMKALG
                     GGWTANLNTPSAPAASASSPTTATAKNDNLTPA"
     gene            complement(4647..>4824)
                     /locus_tag="VIN75_14985"
     CDS             complement(4647..>4824)
                     /locus_tag="VIN75_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8975521.1"
                     /translation="AGREVAAVVPAVLAEVLNLHLAGFSHDEWRALLSMLKRMRANGD
                     ALRAATPPNSATPD"
BASE COUNT          654 a         1611 c         1824 g          735 t
ORIGIN      
        1 gcccgggtcg tctcggcgtc gctagtgcgc cccgcccgcg tccaccgacg gcccgccggc
       61 gatcctgggc gggcgcttgg tgaaccagat cgtgaagatc aggaacagga aggtgacggc
      121 cgaggcgatg aagatgtcgt cggcggcctt ggtgaacgcc tgcacgtcga cgagccggtt
      181 gatgctcgcg taggcgccgg cggtcggcag gccggcctgc gtcagcgccg tggtggcgtt
      241 gtccagcgcg gtgccgttca tcgccatctg ctcgaccagg tgctcgtggt gcatcgtcgc
      301 gcggttgtcc cacagcgtcg tggcgatcga ggtgcccatc gcgcccatcg tgatgcgcac
      361 gaagttcgac aggccggcgg cggcggggat gcgtgacggc ggcaggcccg cgagcgtcag
      421 cgtggtcagc gggatgaaga agaacgccat cgcgccgccc tggatcagcg tcggcagcag
      481 gatggtgccg aagtcggtgt cggacgtgaa cttcgagcgc atccacagca ccagcgcgaa
      541 caggatgaac gcgcccgtgg ccatccagcg cggatcccac ttggccacgt tgcggcccac
      601 gatgggcgtg agcacgatgg ccaggatgcc caccggtgcc agcgccatgc cggccgacgt
      661 ggccgtgtag cccatgtact gctgcagcca cagcggcagc agcaccacgt tgccgaagaa
      721 caggccgtag gcgatggaca gcgtggccgc gccgaacgcg aagttgcgcc ccgcgaacag
      781 ccggatgtcg accaccgggt gctcgtcggt cagctcccac gcgacgaaca ccgcgaaggc
      841 gatcaccacg atcaccgcca gcgtgacgat ggtgggcgag ttgaaccagt cgagctcctt
      901 gcccttgtcc agcatcacct gcaggcagcc cacccacagc accagcatgc ccaggcccac
      961 gccgtcgatg ggccgcttga ccgtcggcgt ctcgcgcttg tggtagatgc tccacgtgat
     1021 gaatgccgcg aagatgccca ccgggatgtt gatgtagaag atccagggcc acgtccagtt
     1081 gtcggtgatg tagccgccca gcaagggccc ggccacgggc gcgaccagcg tcgtcacgcc
     1141 ccacaacgcc aaggccgtgc cggctttctg gggtggatag gtggacagca gcagcgtctg
     1201 cgacagcggg atcatcggcc cggccacgaa gccctgcagc acgcggaacg cgatcagcat
     1261 ctcgatggac ggcgccaggc cgcacagcgt cgaggcgatg acgaacagga tgacgctcag
     1321 cgtgaacagg cgcaccgcgc cgaagcgctg cgtcagccag ccggtgagcg gcacggagat
     1381 ggcgttggcg acgccgaagc tggtgatgac ccaggtgccc tgcgagatgg acacgcccag
     1441 gtcgccggag atcgacggca gggacacgtt cgcgatcgac gagtccagca cgttcatgaa
     1501 ggtcgccagc gacagcgaca gcgtgcccag cacgagcgcg ctgccggtca ggggcgcagg
     1561 cggcttggcc gccgcggcgg gagcggacat tgcgagacct cttgttgttc tggtcggtgg
     1621 gaagcggacg ccggcggcgg cgtccgcgct ctggatcacg actgcttgtg gtggtggcgg
     1681 tgcgaagccg ccgaggcgat cttctccgcg cccgaggccg cggccaccgg gccggacgcg
     1741 gacgacgacg cggcctgcgc cgtcgcggtc ggcgcgatgc ggccgccgcc gttggccgcg
     1801 acgatgcgcg ccacctcggt gtcggcgtcg cgctgcagcg agtcgaacac cgtggtcgag
     1861 gcgacggcgc cctgggtgcg cggcgtgtcg gccaccgagc ggccgctctc gtcggcgacg
     1921 tccaccttca cgttcatcga caggcccacg cgcagcgggt tcttcgccac ttcggccggg
     1981 tccagcgaga tgcgcacggg cacgcgctgc acgaccttga tccagttgcc ggtggcgttc
     2041 tgcgccggca gcagcgagaa cgccgcgccg gtgcccgcgc ccaggccctc gaccttgccg
     2101 tggaagacca cgtgcttgcc gtagacgtcg gcctccagcg tggccggctg gccgatgcgc
     2161 aggcgctcga gctggccttc cttgaagttg gcgtcgaccc aggcgttgtt gagcgtgacc
     2221 accgacatca gcggcacccc cgcctgcacg cgctggccga cctgcacgct gcggcgcgcg
     2281 acggtgccgc tcacgggcgc gggcacgtcg gtgcgctgca ccgcgagcca ggcctcgcgc
     2341 accttggccg cggcgcgctg cacgttgggg tgctgcgcga cgctggtgcc ttcggtctgc
     2401 gactggttcg aggccagctg ttccttggcc gcgtccagcg ccgacttggc ggcggtgtac
     2461 gcgctcctgg ccgattgcag ctgctgctgc gcgtgctcga attcttcctt gccgaccgcg
     2521 ccgctctgca ccagcgggcc gcggcggttg acgtcgtcct gcgccttggc gaggtcggca
     2581 gccgcgcgcg tggcgtcggc ggcgcgggcg tcgatctgcg cctgcagcgt ggagttgttg
     2641 gcgaacagga tgcgcgtctc gcgcaccgtc tgcgccagcg cggcctcggc ctggtcgagc
     2701 gcgacgcgcg cgtcggccgg gtcgagcttg accagcacct ggccggcctt gacgtagtcg
     2761 gtgtcgtcgg cgttgatggc cgtgacggtg ccgccggcct gcgccgtgat ctgcaccacg
     2821 ttggcctgca cgtacgcgtt gtcggtggac tccgagtggc ggccggtcat ccagtagtag
     2881 atgccgtagc cgccgccgat caccagcacg gcgaccgcga tggcggtcag ggcggtgcga
     2941 cgcttggggt tgccggccgg cgcggtcgtg tcgggggcgg cgttgttgtt ggcgggagcg
     3001 ttcatgatgg tttaggcggg cgtgaggttg tcgttcttgg cggtcgcggt cgtcggcgac
     3061 gacgcggagg cggcgggtgc ggacggcgtg ttcaggttgg ccgtccagcc gccgcccagg
     3121 gccttcatca gctgggcctg gacgtccagc gcgcggtact cgaggtcgac ctccaggcgg
     3181 cgctcggcca gcatctgggt ctcggcgttg agcacgatca gcgcgttgcc caggccctgg
     3241 ccatagcgct tctgcgagaa gtcgtagctg gcggtcgagg cctcgagcgc ctggcgctgc
     3301 agcgccagct ggcggccgat cgaggccgcg gaggtggcgg cgtcggcggc ttcgcgcacg
     3361 gcgtcgatca cctgctggtt gtaggcctgg atcgcggcgt cgcgctcggc gcccttgccc
     3421 ttcaactggg cgcgcagctg gccgccctcg aagatgggca ggtgcagcgc ggggccgaac
     3481 gaccaggtct tgctgggcag ctccaggaac ttgcccaggt tgatcgagtc gaggccgaag
     3541 ccggccgaga agttgatgtc cggatagaag cgcgtcttct gcgacgcgac ggcctccttg
     3601 gacgcctcga cgcgcgcgag cgcggcggcg acgtcgggac ggcgcgcgat caggtcggcg
     3661 cccagcaccg gcggctgcgg cgagacatgc agctggtcca gcgtcggcgc gagcctggcg
     3721 gtggcgtcca tcggttgcgc ggtgagcgcg gccagcgcgt ggcgggccag gtcgatctgc
     3781 tcgtcgagcg attcgatctg cgccagcgtg tcgggcagcg cgccctcgcc ctgcttctgt
     3841 tcgacgaccg tgtccaggcc ggcgctgacg cgctgcgtga tcagcgacag catctgcttg
     3901 cgctggtcgt aggtgcgttg cgcgacggcg cgctggctct cgaggcgggc cagcgagaaa
     3961 tacgtgcgca cgacctgcgt ggcgatctgc tgcgcggcca catcggcgtc ggccctggcg
     4021 gcgcgcgcgc tgcccagggc ggactcgagg tcgtgcttct ggcggccgaa cacgtcggcg
     4081 tcccagctca ggccgaaggt ggcgttggac tggttgaaga tgctgccacc gtagggcggc
     4141 gggaacaggc cgttgcgcga gagcagctcg cgcttgaccg aggcgtcgaa ggcgaccgcc
     4201 ggcagctcgg cgccgcgcgc ggcctcgacg accgcctgcg cgcgcgtcag gcgcgcctgc
     4261 gcggagccga ggctgggcga gccctcgagg gccttggcca cgagcgcgtc gagctgggcg
     4321 tcaccgaact ggtgccacca ctgcgcgtcg accgcggtgg tctgctgcga ggccgacagg
     4381 cccagcgcct gggcgtcgcg ctgggcgacg ggctcggact ggtggccggt cgacgcgcag
     4441 ccggccagca ccagcacggc ggccgaggcg acggccagcg cgaaggtgct cgcggacagc
     4501 acgcgcggcg tggccagctc gaggccgcga tgcgtcatct tggccggctt gcgcgagttg
     4561 atggattgcg tctcgccggt cttcggcgac gcgccggcgc ggcgcgggca ttcttgttgt
     4621 cgggtggcgc tgggcatgat gttctttcag tctggggtcg ctgaattcgg gggcgtggca
     4681 gcacgcaggg cgtcgccgtt ggcgcgcatg cgcttgagca tgctcagcag cgcgcgccat
     4741 tcgtcgtgcg agaagccggc caggtgcagg ttgagcacct cggccagcac cgccgggacg
     4801 accgcggcga cctcgcggcc ggca
//
LOCUS       DATCRO010000548         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1085879, whole genome shotgun sequence.
ACCESSION   DATCRO010000548 DATCRO010000000
VERSION     DATCRO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085879"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VIN75_14990"
     CDS             <1..817
                     /locus_tag="VIN75_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8975522.1"
                     /translation="LYNRRVLDRWQLAPPRSWAEMERVAKALRAQHVTPLAWSDEPWQ
                     IATVFETVLLAEGGPRLYAELATTAGWSSWQDARVVAALRRLRWLRDLDDEPTTEEPW
                     TSATRRLQSGAAGMLIMGDWARGELQAWGEHAGLDFDCVAVPQTAGMHLYSIDTLAML
                     VGRNAHRAEQEHMADILAQPATQMAYNQAKGSVPVRTDIDPAQLDACARDSWQTLARP
                     GAPLLPSITHRMAAVESVRDAIGEVLHRFVKSRAMTPQQAQSQLVAIVRGAAQ"
     gene            814..1557
                     /locus_tag="VIN75_14995"
     CDS             814..1557
                     /locus_tag="VIN75_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975523.1"
                     /translation="MTAAPSPGAPRRKSILAVDDDQKTRQLLKQYLEKQQYVVHLAHD
                     GESFRAEFDRYRDELSLVILDVMLPDTDGFALCRLVRERSEVPIIMLTASSDDTERIV
                     GLELGADDYIGKPYNPRELLARIKAIHRRSRTGDAAPRRYMRFAGFCLDRLERTLKDA
                     DGRIVALTGMDFDLLRLFAEHAGEILDRARLMEQTRGRDLGPADRSLDVQVSRLRQRL
                     ADDGKQPVLIKTVRNSGYVFSVEVQSSHD"
     gene            1550..3031
                     /locus_tag="VIN75_15000"
     CDS             1550..3031
                     /locus_tag="VIN75_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975524.1"
                     /translation="MTERATGGWADRLLPATLRGRILLWMVVGVLLSQLLGSAIWTWQ
                     LRDTARRDARDAAHQTAVSAAAAIRFLRDLPGQYRPLLIDQLRQMGGTRFFVAVNGKR
                     VPVAPIEDSLLARTVVGEVRRTLQGELAQGAQADVALAWPETLSVTGDGRLLHELPDS
                     WVVGPMLLRPRRAPVLVIQVDVASGGSLLLASTLPDPYFLDSANPLTRDRLLLQGATL
                     LTVLLLVLAVARTLTRPLQRIADAAAAFGGAMHPSAVPETGTIELRRTARAFNDMQAR
                     IQRFVEDRERLFASISHDLKTPITRLKLRTEMLDDDALRGDFHEDLDELDVMVKGALQ
                     SVKDSAVHENVSDVRLDRLVERLTQPALAAGARIAIDVPALSVRAKPLALKRALGNLV
                     DNGLRYGGNELEIAAAADDAGVVLTIRDHGPGLPASSREEIFEPYVRLAHGRETSRDG
                     SGLGLGIARGILREHGGDVRLDNHPDGGLVATVRLPAGAAPAA"
     gene            3172..>4189
                     /locus_tag="VIN75_15005"
     CDS             3172..>4189
                     /locus_tag="VIN75_15005"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY8975525.1"
                     /translation="MAKNIVLCLDGTWNGPGSKDQRGNLTPTNVQKLFEALAGSAPLA
                     PSDQEKEFTQAASASAVDGQVGKYIYGIGVSTNLLARAVEGAVGLGLVARVVRGYTYL
                     SRAYEPGDRIYILGFSRGAYTARALAGLVTRQGLLDWDAMKLDAGSEQSYSAGLAAWQ
                     QYKKAIYKDDGSVLHSLAGVVSDFQDLFQLGLHPAPQLRYTGAVGIVAVGVWDTVGAL
                     GIPDLAERDGSAVRLDAFQFCDSVLNANVAHGFHAVAIDERRVDFTPTLWEPRDGVVQ
                     VLFAGAHDDVGGGNPSADSGLANVTLAWMARQAASVGVEFARMPVGDPDPLALQHQPW
                     AGTAY"
BASE COUNT          592 a         1558 c         1493 g          546 t
ORIGIN      
        1 gctgtacaac cgccgagtgc tcgatcgctg gcagctcgcg ccgccgcgca gctgggccga
       61 gatggagcgg gtcgccaagg cgctgcgcgc gcaacacgtc acgccgctcg cgtggagcga
      121 cgagccctgg cagatcgcca cggtgttcga gacggtgctg ctcgccgagg gcgggccccg
      181 gctgtatgcg gagctcgcga cgacggcagg ctggtcgtcc tggcaggatg cgcgcgtcgt
      241 cgccgcgctg cggcgcctgc gctggctgcg cgacctcgac gacgagccca ccaccgagga
      301 gccgtggaca tcggccacgc gccggctgca gtcgggcgcg gcgggcatgc tgatcatggg
      361 cgactgggcg cgcggcgagc tgcaggcgtg gggcgagcac gcggggctcg acttcgactg
      421 cgtggccgtg ccgcagaccg ccggcatgca cctgtactcg atcgacacgc tcgccatgct
      481 ggtggggcgc aacgcgcacc gcgcggaaca ggaacacatg gccgacatcc tcgcccagcc
      541 cgccacgcag atggcctaca accaggccaa gggctcggtg ccggtgcgca ccgacatcga
      601 cccggcgcag ctcgacgcct gcgcccgcga ttcgtggcag acgctcgcgc gccccggcgc
      661 gccgctgctg cccagcatca cgcaccgcat ggcggcggtc gaatcggtgc gcgacgcgat
      721 cggcgaggtg ctgcatcgct tcgtgaagtc gcgcgccatg acgccgcagc aggcgcagtc
      781 gcagctcgtg gccatcgtgc gcggggccgc gcaatgacgg ccgcgcccag cccgggcgcg
      841 ccgcgccgca agtccatcct ggccgtcgac gacgaccaga agacgcgcca gctcctcaag
      901 cagtacctcg agaagcagca gtacgtcgtg cacctggcgc acgacggcga gagcttccgc
      961 gccgagttcg accgctaccg cgacgagctg agcctggtga tcctcgacgt gatgctgccc
     1021 gacaccgacg gcttcgcgct gtgccgcctc gtgcgcgagc gctccgaggt gcccatcatc
     1081 atgctgaccg ccagctccga cgacacggag cgcatcgtcg ggctcgagct cggcgccgac
     1141 gactacatcg gcaagcccta caacccgcgc gagctgctgg cccgcatcaa ggcgatccac
     1201 cggcgctcca ggacgggcga cgccgcgccg cgccgctaca tgcgcttcgc cggcttctgc
     1261 ctcgaccgcc tggagcgcac gctcaaggac gccgacggtc ggatcgtcgc gctgaccggc
     1321 atggacttcg acctgctgcg cctgttcgcc gaacacgccg gcgagatcct cgatcgcgcg
     1381 cggctgatgg agcagacgcg cggccgcgac ctcggccccg ccgaccgatc gctggatgtg
     1441 caggtgagcc gcctgcgcca gcgcctggcc gacgacggca agcagccggt gctgatcaag
     1501 acggtgcgca acagcggcta cgtgttctcc gtcgaagtcc agtcgtccca tgactgagcg
     1561 agccaccggc ggctgggcgg accggctgct gccggcgacg ctgcgcggac gcatcctgct
     1621 gtggatggtg gtcggcgtgc tgctgagcca gctgctgggc agcgcgatct ggacctggca
     1681 gctgcgcgac accgcccgcc gcgacgcgcg cgatgccgcg caccagaccg ccgtcagcgc
     1741 cgccgccgcc atccgcttcc tgcgcgacct gcccggccaa taccgcccgc tgctgatcga
     1801 ccagctgcgc cagatgggtg gcacgcgctt cttcgtggcc gtcaacggca agcgcgtgcc
     1861 ggtggcgccg atcgaggact ccctgctcgc gcgcaccgtc gtcggcgagg tgcgccgcac
     1921 gttgcagggc gagctcgcgc agggcgcgca ggcggacgtg gcgctcgcgt ggcccgagac
     1981 gctgtcggtc accggcgacg gccgcctgct gcacgagctg cccgacagct gggtcgtcgg
     2041 cccgatgctg ctgcgcccgc gccgcgcgcc ggtgctcgtc atccaggtgg atgtcgcctc
     2101 cggcggcagc ctgctgctgg cctccaccct gcccgacccg tacttcctgg acagcgccaa
     2161 cccgctcacg cgcgaccgcc tgctgctgca aggcgccacg ctgctgacgg tgctgctgct
     2221 ggtgctggcc gtcgcgcgca cgctcacgcg accgctgcaa cgcatcgccg acgcggccgc
     2281 ggccttcggc ggcgcgatgc acccgagcgc cgtgcccgag accggcacca tcgagctgcg
     2341 ccgcacggcg cgcgcgttca acgacatgca ggcgcgcatc cagcgcttcg tggaggatcg
     2401 cgagcgcctg ttcgcgagca tctcgcacga cctgaagacg cccatcacgc ggctgaagct
     2461 gcgcaccgag atgctggacg acgatgcgct gcgcggcgac ttccacgagg atctggacga
     2521 gctcgacgtg atggtcaagg gcgcgctgca aagcgtcaag gacagcgcgg tgcacgagaa
     2581 cgtcagcgac gtgcgcctcg atcgcctcgt cgaacgcctg acgcagcccg cgctcgcggc
     2641 cggcgcgcgc atcgccatcg acgtgcccgc gctgagcgtg cgcgccaagc cgctggcgct
     2701 caagcgcgcg ctcggcaacc tcgtcgacaa cggcctgcgc tacggcggca acgagctcga
     2761 gatcgccgcc gcggccgacg acgcgggcgt cgtgctcacg atccgcgacc acgggcccgg
     2821 cctgcccgcg agctcgcgcg aggagatctt cgagccgtac gtgcggctcg cgcacggccg
     2881 cgagacgagc cgcgacggca gcgggctggg cctgggcatc gcgcgcggca tcctgcgcga
     2941 gcacggcggc gacgtgcgcc tggacaacca ccccgacggc ggcctcgtgg ccaccgtgcg
     3001 cctgcccgcc ggcgccgcgc cagccgcctg agcgtcgccc cgcttcgtat tcggggggcc
     3061 ggccgcccgg tcgcgatcct ggggatgggt gcgccgcacc ggtcctttga tgatgagcac
     3121 gccgcgcggc cgccctcgcg cgccgcggct gcgcatcgga ggactgcgac catggccaag
     3181 aacatcgtgc tgtgcctgga cggaacctgg aacggccccg gcagcaagga ccagcgaggc
     3241 aacctgacgc ccacgaacgt gcagaagctg ttcgaggcgc tggccggctc ggcgccgctc
     3301 gcgccgtccg accaggaaaa ggagttcact caggcggcct cggccagcgc ggtcgacggc
     3361 caggtcggca agtacatcta cggcatcggc gtcagcacca acctgctcgc cagggccgtg
     3421 gagggcgcgg tcggtctcgg cctggtggcg cgcgtcgtgc gcggctacac gtacctgtcg
     3481 cgcgcgtacg agccgggcga ccgcatctac atcctgggct tcagccgcgg cgcctacacg
     3541 gcgcgcgcgc tggccggcct ggtcacgcgc caggggctgc tcgattggga cgccatgaag
     3601 ctggacgccg gctcggagca gagctacagc gcgggcctgg ccgcgtggca gcagtacaag
     3661 aaggccatct acaaggacga cggcagcgtg ctgcacagcc tggcgggcgt cgtgagcgac
     3721 ttccaggacc tgttccagct cgggctgcat ccggcgccgc agctgcgcta caccggcgcg
     3781 gtcggcatcg tggccgtcgg cgtctgggac acggtgggcg cactcggcat tcccgacctc
     3841 gcggaacggg acggctcggc cgtgcggctc gacgcgttcc agttctgcga ctcggtgctc
     3901 aacgccaacg tcgcccacgg cttccacgcg gtggccatcg acgagcgccg cgtcgacttc
     3961 acccccacgc tgtgggaacc gcgcgacggc gtggtgcagg tgctgttcgc cggcgcgcac
     4021 gacgatgtcg gcggcggcaa tccctccgcc gactcgggcc tggccaacgt gacgctcgcg
     4081 tggatggcgc ggcaggccgc gtcggtgggc gtcgagttcg cgcgcatgcc cgtgggcgat
     4141 cccgacccgc tggcgctgca gcaccagccg tgggccggca cggcgtacc
//
LOCUS       DATCRO010000549         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_714961, whole genome shotgun sequence.
ACCESSION   DATCRO010000549 DATCRO010000000
VERSION     DATCRO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714961"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VIN75_15010"
     CDS             <1..970
                     /locus_tag="VIN75_15010"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY8975526.1"
                     /translation="AFIPPSIAARRPAPEPAPAPPPAVQAALPLVTPIVEEPAAPTAS
                     ERAEDFAAPPPPDSALAEPSVIDEFDEFRELGDLAALEVVEPAPEVGGSTIPSRLADD
                     LEEEHRAGPPTLASMLPEDAGEWPPRKTKKRKGKDAVEGTPESVLVDKTKDPRFLREA
                     RSGARWRKPWVRATLSLLLVVLIAGAVAQVAWPLRDTLAARWPVTQPMWAWLCEQADC
                     KIEAPRAIASLALDGSSLTRTDTEHVLLFSADLHNRADHEVLMPSFDVSFMDLNGAIV
                     ARKVLSPEQLGIHQASLPALGELHVHARLQVGTLPASGFQADLFYP"
     gene            1032..1937
                     /locus_tag="VIN75_15015"
     CDS             1032..1937
                     /locus_tag="VIN75_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY8975527.1"
                     /translation="MASLVCGSLAFDTITVFPGRFADQILPEQLHILNVSFLVPTLRR
                     EFGGCAGNIAYTLKLLGGEPVVLAALGNDGASYLAHLDKLGIAKDGVHVAADNYTAQA
                     IIITDAANNQITAFHPGAMMQAHEAGLPARSDIRLAIIGPDGRDAMIRRATDLAAAKI
                     PFIFDPGQGLPMFDGADLRAFIEQATWVTVNDYEARMLTDRTGLSLAELSTSHLKGVI
                     VTLGDQGCEVWEQGVRTHVPGEKASAVVDPTGCGDAFRGALLYGLEKGWSLIDCVKLG
                     NKVGALKIAHRGGQNHPIDKAALGV"
     gene            complement(2135..2473)
                     /locus_tag="VIN75_15020"
     CDS             complement(2135..2473)
                     /locus_tag="VIN75_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8975528.1"
                     /translation="MTHELDSSPAWPCLSVRGDGVLLQVSVMPNAKRTEVDGLHDGAL
                     RVRLAAPPIEGRANDALVAWLAKSLGVARRDVELVRGESSRRKQVAIAASYDVVARWL
                     DGQCRHSRPR"
BASE COUNT          391 a          961 c          861 g          335 t
ORIGIN      
        1 ggcgttcatt ccgccgtcga tcgcggcgcg ccgccccgca cccgaaccgg cgccagcccc
       61 gccgcccgcg gtgcaagccg cgctgccgct cgtcaccccg atcgtcgagg agccggccgc
      121 cccgaccgcc tcggagcgcg ccgaggactt cgcggccccg ccgccgccgg actcggcgct
      181 ggccgagccg agcgtcatcg acgagttcga cgagttccgc gagctcggcg atctggccgc
      241 gctcgaggtg gtggagccgg cgcccgaagt cggtggctcg accatcccca gccggctcgc
      301 cgacgacctc gaggaagagc atcgcgccgg cccgcccacg ctggcgtcga tgctgcccga
      361 ggacgccggc gagtggccgc cgcgcaagac gaagaagcgc aaaggcaagg acgccgtcga
      421 gggcacgccc gagagcgtgc tcgtcgacaa gaccaaggac ccgcgcttcc tgcgcgaggc
      481 ccgcagcggc gcgcgctggc gcaagccgtg ggtgcgtgcg acgctgagcc tgctgctggt
      541 ggtgctcatc gcgggtgccg tcgcgcaggt ggcctggccg ctgcgcgaca ccctcgccgc
      601 gcgctggccc gtcacgcagc cgatgtgggc ctggctgtgc gagcaggccg actgcaagat
      661 cgaggcaccg cgcgcgatcg cgagcctcgc gctcgacggc agctcgctga cccgcaccga
      721 caccgagcac gtgctgctgt tctccgccga cctgcacaac cgcgccgacc acgaggtgct
      781 gatgccctcg ttcgacgtca gcttcatgga cctcaacggc gcgatcgtcg cgcgcaaggt
      841 gctcagcccc gagcagttgg gcatccacca ggcgtcgctg ccggcgctgg gcgagctgca
      901 tgtgcacgcg cggctgcagg tcggcacgct gccggccagc ggtttccagg ccgacctgtt
      961 ctatccctga cccgaacccg cggcgcggcg cacgcgcgcc cgcgtcaccc accgactcct
     1021 gaaagcgccc catggccagc ctcgtctgcg gttccctcgc gttcgacacc atcaccgtct
     1081 tccccggccg cttcgcggac cagatcctgc ccgagcagct gcacatcctg aacgtgtcgt
     1141 tcctggtgcc gacgctgcgg cgcgagttcg gcggttgcgc gggcaacatc gcctacacgc
     1201 tcaagctgct gggcggcgag ccggtggtgc tcgccgcgct cggcaacgat ggcgcgagct
     1261 acctcgcgca cctggacaag ctgggcatcg ccaaggacgg cgtgcacgtg gccgcggaca
     1321 actacaccgc gcaggcgatc atcatcaccg acgccgccaa caaccagatc accgcgttcc
     1381 accccggcgc gatgatgcag gcgcacgagg ccggcctgcc cgcgcgcagc gacatccgcc
     1441 tggcgatcat cggccccgac ggccgcgacg cgatgatccg ccgcgcgacc gacctggccg
     1501 ccgccaagat cccgttcatc ttcgacccgg gccagggcct gccgatgttc gacggcgccg
     1561 acctgcgcgc cttcatcgag caggcgacct gggtcacggt caacgactac gaggccagga
     1621 tgctcaccga ccgcaccggc ctgagcctgg ccgagctgtc gacctcgcac ctgaagggcg
     1681 tgatcgtgac gctgggcgac cagggctgcg aggtgtggga gcaaggcgtg cgcacgcacg
     1741 tccccggcga gaaggccagc gccgtcgtcg atccgaccgg ctgcggcgac gcgttccgcg
     1801 gcgcgctgct gtacgggctc gagaagggct ggtcgctgat cgactgcgtc aagctcggga
     1861 acaaggtcgg ggcgctcaag atcgctcatc gcggcggaca gaaccacccg atcgacaagg
     1921 ccgcgctcgg cgtctgatcg gcgacctgtc ggtcggcgcg agcacgcaag gccgtcattg
     1981 cgcgtcgttg cagggcggtc attccacacg gagtcattgc ggcgtcattc cgcgcgcagt
     2041 cgcggaatcc accttcgcga gcaaacggcg cctcgctacc ggcgtctccc tggcccaccg
     2101 cggtggatcc tgcgacttcg cgcaggatga cgagtcagcg gggccgcgaa tggcgacact
     2161 gcccgtcgag ccagcgcgcg acgacgtcgt acgaagccgc gatcgccacc tgcttgcgcc
     2221 ggctggactc gccgcgcacc aactcgacat cgcgcctggc cacgcccagc gacttcgcca
     2281 gccacgccac cagggcgtcg ttcgcacgcc cctcgatcgg cggcgcggcc agccgcacgc
     2341 gcagcgcgcc gtcgtgcagg ccatcgacct cggtccgctt cgcgttgggc atgacgctga
     2401 cctgaagcag cacaccgtcg ccgcgcacgg acaggcaagg ccaggccggc gacgaatcca
     2461 gttcatgcgt catggggtcg tgagccggaa agaaaaagcc cggccgaggc cgggcttcca
     2521 gtcaagcgag aacgctgacc tcagggct
//
LOCUS       DATCRO010000550         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_557828, whole genome shotgun sequence.
ACCESSION   DATCRO010000550 DATCRO010000000
VERSION     DATCRO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557828"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VIN75_15025"
     CDS             complement(<1..1495)
                     /locus_tag="VIN75_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8975529.1"
                     /translation="MTTVEPKLEPTPIVARPWPVRREPKGSWLARTLRTTDAKQIGLL
                     YFVTSFAFFAIGGVLALLIRAELFQPGLQIVSSEQFNQLFTMHGTIMLLMFATPIVFA
                     FGNYLVPLQIGAPDVAFPRLNAFAYWLYLLGSTLAVAGFITPGGAASFGWFAYQPLSN
                     AVNSPGIGADMWFVGLSISGAGTILGAVNMITTIVTMRAPGMLMFRMPIFTWNILITS
                     LLMILVFPILFAALLAVMADRTLGAHVFDADTGGAILWQHLFWFFGHPEVYIVALPFF
                     GIVSEIFPVFSRKPIFGYKTLVAATLAIAGLSMAVWAHHMYATGAVMLPFFSLMTFLI
                     AVPTGVKFFNWIGTMWKGQLTFEAPMLFSIGFLVTFLLGGLSGVLLASPPVDFHVSDS
                     YFVVAHFHYVLFGTIVFAVYAGVYFWFPKVAGRMYDERLAKVHFWLTFIGFHTTFLIQ
                     HWIGVMGGARRYWSYLAEDGLTWMNQISTVGAGILAVSLIPFFLNVLV"
     gene            complement(1724..2131)
                     /locus_tag="VIN75_15030"
     CDS             complement(1724..2131)
                     /locus_tag="VIN75_15030"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY8975530.1"
                     /translation="MVDSNDKNVGELDWLLTSFVEKVPDVSEALTVSVDGLAIAASRG
                     LSTDRADQLAAITSGLASLTTGAAKVLDAGRIRQSVIDMDEGVLLLMAVGDRAQLAVL
                     ANAGCDLGQVGYETALLVKRVYAALEPDARGGI"
     gene            complement(2143..>2881)
                     /locus_tag="VIN75_15035"
     CDS             complement(2143..>2881)
                     /locus_tag="VIN75_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975531.1"
                     /translation="PTQSVRVTSRRSGSEIVISIADDGIGLAPEPMAAINRRLAEPTQ
                     LNSELTATMGLLVVGRLAAEHSVSVQLHSTMGKGTLALVRIPASAHAEDPVGLSPDQG
                     SAARLALSARAANLPVTGTGAEAAGSGAVEPIVPRPRKAGPAPAPRPVAGTTLNGGKP
                     VAAPAGPAPETLDVTFRKVNRALENSGRTNGAELPRRAPGSRLLPGSVPSARQGGGAD
                     LDPAEIRDRLAGFASGIAAAAAHEQRQ"
BASE COUNT          501 a          952 c         1034 g          394 t
ORIGIN      
        1 tgacgagcac gttgaggaag aacgggatca gcgagaccgc gaggatgccg gcgccgacgg
       61 tcgagatctg gttcatccag gtcagcccgt cctccgcgag gtagctccag tagcggcggg
      121 cgccgcccat gacgccgatc cagtgctgga tgaggaacgt cgtgtggaag ccgatgaacg
      181 tcagccagaa gtggaccttc gcgaggcgct cgtcgtacat gcgcccggcg accttcggga
      241 accagaagta cacgccggcg tagaccgcga acacgatcgt gccgaacagc acgtagtgga
      301 agtgcgcgac cacgaagtac gagtcggaga cgtggaagtc gaccggcggg ctggcgagca
      361 ggacgcccga gaggccgccg aggaggaacg tcaccaggaa gccgatgctg aacagcatcg
      421 gggcctcgaa cgtcagctgg cccttccaca tcgtgccgat ccagttgaag aacttcacac
      481 cggtcgggac cgcgatgagg aacgtcatga gcgagaagaa cggcagcatg accgcgccgg
      541 tcgcgtacat gtggtgcgcc cacaccgcca tcgacaggcc cgcgatggcg agcgtcgcgg
      601 cgaccagggt cttgtagccg aagatcggct tgcggctgaa caccgggaag atctccgaga
      661 cgatgccgaa gaacggcagc gccacgatgt acacctcggg gtggccgaag aaccagaaca
      721 ggtgctgcca caggatcgcg ccgccggtgt cggcgtcgaa cacgtgcgcg ccgagcgtgc
      781 ggtcggccat gacggcgagc agcgcggcga acaggatcgg gaagaccagg atcatcagca
      841 ggctggtgat gaggatgttc cacgtgaaga tcggcatccg gaacatgagc atgcccggcg
      901 cgcgcatcgt cacgatcgtg gtgatcatgt tgacggcgcc gaggatcgtg cccgcacccg
      961 agatcgacag gcccacgaac cacatgtccg cgccgatgcc gggggagttc acggcgttcg
     1021 acagcggctg gtaagcgaac cagccgaagg acgccgcgcc gcccggggtg atgaagccgg
     1081 cgaccgcgag ggtcgagccg agcaggtaca gccagtacgc gaacgcattc aggcgcggga
     1141 acgcgacgtc gggggcgccg atctggagcg gcaccaggta gttgccgaac gcgaacacga
     1201 tcggggtcgc gaacatcagc agcatgatgg tgccgtgcat cgtgaacagc tggttgaact
     1261 gctcagagga cacgatctgg agtccgggct ggaacagctc cgcgcggatc agcagggcca
     1321 ggacgccgcc gatcgcgaag aacgcgaacg acgtcacgaa gtagagcagg ccgatctgct
     1381 tcgcgtcggt cgtgcggagc gtgcgggcga gccaggagcc cttgggctcg cggcgcaccg
     1441 gccagggacg cgcgacgata ggcgttggct caagcttcgg ctcaaccgtg gtcacctgtg
     1501 ggcctccatg attcatttcc aggtagcgaa cgcagcatag ccccgtgaaa cgccgcctac
     1561 gcggaggggc gcagcctcag cggcgtggcg cagcgtgacc gttcgatggg cgctcagtgg
     1621 ttgtcgcagg tcgaatacgc gacaggtcat aggggtggaa ccgcccgcgc gggccgtcga
     1681 tgggcgcggc ccggcgcggg tgagcaatct cctacgcggc cggtcagatc ccgccgcggg
     1741 catcgggttc gagcgcggcg tagacgcgct tcaccagcag cgcggtctcg tagccgacct
     1801 ggccgaggtc gcagccggcg ttggcgagga ccgcgagctg ggcccggtcg ccgacggcca
     1861 tgagcagcag cacgccctcg tccatgtcga tcaccgactg gcggatgcgg cccgcgtcga
     1921 ggaccttcgc ggcccccgtc gtcaggctcg cgagccccga ggtgatcgcg gcgagctggt
     1981 cggcccggtc cgtcgacagt ccccttgagg cggcgatggc gaggccgtcg acggacaccg
     2041 tgagcgcctc cgagacgtcc ggcaccttct ccacgaagga ggtcaggagc cagtcgagtt
     2101 caccgacgtt cttgtcgttg ctatccacca tctcgctgct ttctattggc gttgttcgtg
     2161 cgcggcggcc gccgcgatgc cggaggcgaa accggcgagc cggtcccgga tctccgccgg
     2221 gtcgagatcg gcgccgccgc cctgccgggc gctggggacg gagccgggga gcagtcggga
     2281 gcccggcgcg cgacggggca gttcggcgcc gttggtcctg ccggagtttt cgagtgctcg
     2341 gttcaccttc ctgaaggtaa catccagcgt ctcgggagcg gggcccgcag gggccgcgac
     2401 cggcttgcct ccgttcagtg tcgtcccagc tacgggacgt ggcgcggggg ccggaccggc
     2461 cttgcgcggg cgcggcacga tcggctccac ggcgcccgat ccggccgcct cggcgcccgt
     2521 cccggtcacc ggcaggttcg ccgcccgcgc cgacagcgcc agccgcgccg ccgaaccctg
     2581 gtcgggcgac aggcccaccg ggtcctccgc gtgcgcgctc gccggaatcc gcaccagcgc
     2641 cagcgtgccc ttgcccatcg tcgaatgcag ctgcacgctc accgagtgct ccgcggccag
     2701 ccgccccacc accaacaggc ccatcgtcgc cgtcagctcc gagttcagct gcgtcggctc
     2761 ggccaggcgg cggttgatcg ccgccatcgg ctccggcgcc aggccgatgc cgtcgtcggc
     2821 gatcgagatg acgatctccg aacccgaacg gcgcgaggtg acccgcaccg actgggtcgg
     2881 c
//
LOCUS       DATCRO010000551         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1052243, whole genome shotgun sequence.
ACCESSION   DATCRO010000551 DATCRO010000000
VERSION     DATCRO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052243"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VIN75_15040"
     CDS             complement(<1..799)
                     /locus_tag="VIN75_15040"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY8975532.1"
                     /translation="MTPRRLLDTPDDFALFDDATTRRIERRAAAALPAHALMARAGLA
                     LLRLARAIAPNARAAWIAAGPGNNGGDGLVLATWLHRAGMDVRVTLVGHRADRALPAD
                     AAWALAEARAAGVAMTSELPAAADIGDIDLAVDALLGVGQSRPPEGAMADAIALLNAL
                     RCPVLAVDAPTGLSTDTGAQLGDAAVRATHTLTLLTAKPGLFTGAGRELAGDPWFDDI
                     GVTPADDEPPTASLTGAGTAANMTPPRRHLANKGSYGDVHVAGGAAGM"
     gene            complement(809..2077)
                     /locus_tag="VIN75_15045"
     CDS             complement(809..2077)
                     /locus_tag="VIN75_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8975533.1"
                     /translation="MRIAVIGAGMAGVAAAQALAADGHEVTVFERRSSVAEESSFAPA
                     GIAAAALAAPWRPAPGRLQAGKADDVSRLHPTGAAFAVPGLLGKGDAAATARREERAR
                     ALHRLLAAGVARIDELTRALGLDFERSDGLLLVQPDAAQATHAIAWLQALGETAEWAD
                     AVRQRQLEPGRGVADDAPRAVWLPQARVGNPREWTQLLRAHAVHLGARFQFQHVVQAI
                     SAGPKPAVRFSAADGQTSEAEFDAVVVCAALESARLLAPLGVALPCKPVRGYSVTVAQ
                     HVRESAPDSGPRAALLDVRSGVSVTRLGARIRVAGGHEAVPVPAPGGAPVEPPGRRQV
                     QPLYEAVDGAFPGAANWQQAQVWQATRAALPDGLPAVGASGVPGVWLDVGHAHHGWAL
                     SQASAGLLCTLLAGEPAPPEAAALAPQRLR"
     gene            2372..3118
                     /gene="rpsB"
                     /locus_tag="VIN75_15050"
     CDS             2372..3118
                     /gene="rpsB"
                     /locus_tag="VIN75_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8975534.1"
                     /translation="MSVSMREMLEAGVHFGHQTRFWNPKMAPYIYGHRNKIHIINLEK
                     TLPAFENALKFIRTLAAKRGTILMVGTKRQAREVVAAEAQRCGMPYVDQRWLGGMLTN
                     FKTVKGSLKKLKDMQAAKDSGTLAVIKKEALMFERERIKLEKDIGGIQDMAALPDAIF
                     VIDVGYHKIAIQEANKLGIPVIGVVDTNHSPIGIDYVIPGNDDSAKAVALYAKAVADA
                     VVEGKANATNDVVQQVAASGDEYVEESAEG"
     gene            3284..4159
                     /gene="tsf"
                     /locus_tag="VIN75_15055"
     CDS             3284..4159
                     /gene="tsf"
                     /locus_tag="VIN75_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8975535.1"
                     /translation="MPAITASMVAELRAKTDAPMMLCKKALTEADGDLAKAEEILRVK
                     LGTKAAGAGSRVTAEGVIATYVAGNVGAMIEVNCETDFVTKNDDFIAFANKLAKLVAE
                     KNPADVAALSALELDGSTVDAVRLALVGKIGENMTIRRFERVENAGLAHYLHGTRIGV
                     VVQFEGDDTAAKDVAMHVAAMKPKALSAADVPAHLVETERSVATQKAAESGKPADIVA
                     KMVEGSVQKYLKEVSLLNQSFVKNDKQTVEQMLKAANTTIKGFTLYVVGEGLEKKADN
                     FADEVAAQVAAARGH"
     gene            4278..>4543
                     /locus_tag="VIN75_15060"
     CDS             4278..>4543
                     /locus_tag="VIN75_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8975536.1"
                     /translation="MPAYKRILLKLSGEALMGDDAFGINRATIVRMVREVQEITQLGC
                     EVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNSLALADT"
BASE COUNT          726 a         1608 c         1619 g          590 t
ORIGIN      
        1 ccatgcccgc cgcgccgccg gcgacgtgca cgtcgccgta gctgcccttg ttggcgaggt
       61 ggcggcgcgg cggcgtcatg ttcgcggccg tgcccgcgcc cgtgagcgac gcggtcggcg
      121 gttcgtcgtc cgcgggcgtg acgccgatgt cgtcgaacca cgggtcgccc gcgagctcgc
      181 ggcccgcgcc cgtgaacagg ccgggcttgg ccgtcagcag cgtgagcgtg tgcgtggcgc
      241 gcacggcggc gtcgccgagc tgggcgccgg tgtccgtcga cagcccggtg ggcgcatcga
      301 cggcgagcac cgggcagcgc agcgcgttca agagggcgat ggcatcggcc atggcgccct
      361 cggggggacg cgactggccc acgcccagca gcgcgtcgac ggcgaggtcg atgtcgccga
      421 tgtccgccgc agccggaagt tcggacgtca tggcgacgcc cgcggcgcgc gcctcggcca
      481 gcgcccaggc cgcgtcggcg ggcagcgcgc gatccgcgcg atggccgacc agcgtcacgc
      541 gcacgtccat gccggcgcga tgcagccacg tcgccagcac gaggccgtcg ccgccgttgt
      601 tgccggggcc ggccgcgatc caggccgcgc gggcgttcgg cgcgatcgca cgcgccaggc
      661 gcagcagcgc gaggccggcg cgcgccatca gcgcatgggc gggcagagcg gccgcggcac
      721 gccgctcgat ccgccgcgtt gtcgcatcgt cgaacaacgc gaagtcgtcg ggggtgtcga
      781 gcaggcggcg cggcgtcatc ggggcaggtt aacgcaatcg ctgcggggcg agggcggccg
      841 cttccggcgg cgccggttcc ccggccagca gcgtgcagag cagcccggcg ctggcctggc
      901 tcagcgccca gccgtggtgg gcgtggccga cgtccagcca gacacccggc acgccgctgg
      961 cgcccaccgc gggcaggccg tcgggcagcg ccgcgcgcgt cgcctgccag acctgcgcct
     1021 gctgccagtt cgcggcgccg gggaatgcgc cgtcgacggc ctcgtacagc ggctgcacct
     1081 ggcggcggcc cggcggttcg accggcgcgc caccgggcgc gggcaccggc acggcctcgt
     1141 gcccgccggc gacgcggatg cgcgcgccga gccgggtgac ggacacgccg ctgcgcacgt
     1201 ccagcaacgc ggcgcgcggg ccgctgtcgg gcgccgactc gcgcacgtgc tgcgccacgg
     1261 tgaccgagta gccgcgcacc ggcttgcacg gcagcgcgac gcccagcggc gccagcaggc
     1321 gcgccgactc gagcgcggcg cacacgacga ccgcatcgaa ctcggcctcg ctcgtctggc
     1381 cgtcggccgc cgagaagcgc acggccggct tcgggccggc agagatggcc tgcaccacgt
     1441 gctggaactg gaagcgggcg cccaggtgca cggcgtgcgc ccgcagcagc tgcgtccatt
     1501 cgcgcgggtt gcccacgcgc gcctgcggca gccagacggc acgcggcgcg tcgtcggcga
     1561 cgccgcggcc gggctcgagc tggcgctggc gcacggcgtc ggcccactcg gccgtctcgc
     1621 cgagcgcctg caaccacgcg atcgcgtgcg tggcctgcgc ggcgtcgggc tgcaccagca
     1681 gcaggccgtc gctgcgctcg aaatccaggc ccagcgcgcg ggtgagctcg tcgatgcgcg
     1741 cgacgccggc ggccagcagc cggtgcagcg cccgcgcgcg ctcctcgcgg cgtgcggtcg
     1801 ccgcggcgtc gcccttgccc agcaggcccg gcacggcgaa ggcggcgccg gtcgggtgca
     1861 gccggctgac gtcgtccgcc ttgcccgcct gcaaccgccc gggcgcgggc cgccatggcg
     1921 cggcgagcgc ggccgcggcg atgccggcgg gggcgaagct ggactcctcg gcgacgctgg
     1981 agcggcgctc gaacaccgtc acctcgtggc cgtcggcggc cagggcctgg gcggccgcga
     2041 cgccggccat gccggcgccg atcacggcga tcctcatcgg gcggcctccg cgagataaat
     2101 gtgcggggcg ggggtggaac tggtgcagga ccgcgtctgg cctgctatac tctttgggtt
     2161 tttctcggtg gccatgcaaa agggtgacaa cccggcgcgg tgatcgatga aaacggggtc
     2221 ttttcagcga tgaaagatcc caaacacgcg aacccggcaa tcaaggtgtt gtggtcgagc
     2281 aggcgcaggg cctgaggcgg ttagaaaccg taagtcctgt agctcgatgc aatgtgtgcc
     2341 gggattctag acccaacctt tggagtactc catgtctgta tccatgcgcg aaatgctgga
     2401 agccggcgtc catttcggac accagacccg cttctggaac cccaagatgg ccccgtacat
     2461 ctacggccat cgcaacaaga tccacatcat caatctcgag aagaccctgc cggccttcga
     2521 gaacgcgctc aagttcatcc gcaccctcgc ggccaagcgc ggcacgatcc tgatggtggg
     2581 caccaagcgc caggcgcgcg aagtggtcgc cgccgaagcc cagcgctgcg gcatgcccta
     2641 tgtcgaccag cgctggctgg gcggcatgct gaccaacttc aagacggtca agggttcgct
     2701 caagaagctc aaggacatgc aggccgccaa ggattcgggc acgctggccg tgatcaagaa
     2761 ggaagccctg atgttcgagc gcgagcgcat caagctcgag aaggacatcg gcggcatcca
     2821 ggacatggcc gcgctgccgg acgcgatctt cgtgatcgac gtcggctacc acaagatcgc
     2881 catccaggaa gccaacaagc tgggcatccc ggtcatcggc gtcgtggaca ccaaccattc
     2941 gccgatcggc atcgactacg tgatcccggg caacgacgac tcggccaagg ccgtggcgct
     3001 gtacgccaag gccgtggctg acgccgtggt cgaaggcaag gccaacgcga cgaacgacgt
     3061 ggtgcagcag gttgccgcct cgggcgacga gtacgtcgaa gagagcgccg aaggctgatc
     3121 acccccaccc gtcgcctgac gggtctgtgt tcaagggggg ctgcgaggcc ccctttgcac
     3181 aaggcgaggc gaaagcgcaa cagtcgctcg ccgcgccgag gacggaagca atttgttcac
     3241 tgtcggccag tacgctggcc ggcgcagatg atggagtttg aagatgcccg cgattaccgc
     3301 aagcatggtg gctgaactgc gcgccaagac cgacgcgccg atgatgctct gcaagaaggc
     3361 cctgaccgag gccgacggcg acctggccaa ggccgaagag atcctgcgcg tcaagctggg
     3421 caccaaggcg gccggcgccg gttcgcgcgt caccgccgaa ggcgtcatcg ccacctacgt
     3481 cgccggcaac gtcggcgcca tgatcgaagt caactgcgag accgacttcg tcaccaagaa
     3541 cgacgatttc atcgccttcg ccaacaagct ggccaagctg gtcgccgaga agaacccggc
     3601 cgacgtcgcc gcgctgtcgg cgctcgagct ggacggctcg acggtcgacg ccgtgcgcct
     3661 ggcgctggtc ggcaagatcg gcgagaacat gaccatccgc cgcttcgagc gcgtggagaa
     3721 cgccggcctg gcgcactacc tgcacggcac gcgcatcggc gtggtcgtcc agttcgaggg
     3781 cgacgacacg gccgccaagg acgtcgcgat gcacgtcgcc gcgatgaagc cgaaggcgct
     3841 gtccgccgcc gacgtcccgg cccacctggt cgagaccgag cgctcggtgg ccacccagaa
     3901 ggccgccgag tccggcaagc cggccgacat cgtcgccaag atggtcgaag gctcggtgca
     3961 gaagtacctc aaggaagtct cgctgctgaa ccagtcgttc gtgaagaacg acaagcagac
     4021 cgtcgagcag atgctcaagg ccgccaacac gacgatcaag ggcttcacgc tctacgtcgt
     4081 cggcgagggc ctggagaaga aagccgacaa cttcgccgac gaggtcgcgg cccaggtcgc
     4141 ggcggcccgc gggcactgaa cgcggcatcg cggccccggg cgggggccgc ggcttacact
     4201 cacgcggtcc ctgccatccg ccccatcgac agcggctctt ttccagcgtc ccaccagccc
     4261 cgaaagtcct gccctccatg ccggcgtaca agcgcatcct tctgaaactt tccggcgaag
     4321 cgctgatggg cgacgacgcc ttcggcatca atcgcgccac catcgtgcgc atggtgcgcg
     4381 aggtccagga gatcacgcag ctcggctgcg aggtcgcggt cgtcatcggc ggcggcaaca
     4441 tcttccgcgg cgtggccggc ggctccgtcg gcatggaccg cgccacggcc gactacatgg
     4501 gcatgctcgc gaccgtgatg aactcgctcg ccctggccga cac
//
LOCUS       DATCRO010000552         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_523521, whole genome shotgun sequence.
ACCESSION   DATCRO010000552 DATCRO010000000
VERSION     DATCRO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523521"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2113
                     /locus_tag="VIN75_15065"
     CDS             <1..>2113
                     /locus_tag="VIN75_15065"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8975537.1"
                     /translation="GMCVTSLNGVLWLAVAGAPESAHLLPAWIGWLVGDAVGALLAGV
                     PLIAWSRASAALAFGGPAGLSNLLLQAVVVACGLTAFSGWLDRDSAFVYPLLALPFFL
                     LALLAMRGGVIAASIGVLLLSIATGYGTARGVGPFALHDPRAGSLALWSYVTAQACTS
                     LLICSLGMALQASQRQFEAFLQNVSDGILVIDDQGVLLHANAAFTAMTGTDAATLVGR
                     RAADAMRGGAGAIAALVAGGAPGSTTELTLPRAGGGRRVVECQVARYRKASGYWQTHV
                     TLRDITQRVEAQEQLATSEARLKAVTDNVPAMFAYVDRDQVYRFANGHFRHVMGAEPE
                     SVIGRTMRQFLGDAAHAELLPHIEGALRGEPQQFERTGWRENANRHFLAQYVPEVRPD
                     GSVPGFFIMVLDITERFRAELALARSEALVRAIADHMPGLISRMDRDYRYTFVNANYM
                     SWFSLPESPVGKTVAEVFGQDVFEGVHARIDQALAGHDVVFDLTNKVPGGVDFMQVHY
                     VPDRDERGQVIGVYGLVTDRTEQQHARDRIEASERQLRAVTDNLPMLITYVDADERLR
                     FMNATFHVWLGTDIAAAIGRPLRDVVGEEHYAARRDHLRAALAGRRVEFEVVSRTLQG
                     LRNLQTVYIPDVRDDGSVRGIFTMSTDVTSLKETERELQRLARVDTLTGLANRRQFDE
                     LLEQSLARYRRARRPLALIYLD"
BASE COUNT          277 a          761 c          768 g          307 t
ORIGIN      
        1 gggcatgtgc gtcacctcgc tcaacggcgt gctgtggctg gccgtggccg gcgcgccgga
       61 gtccgcgcac ctgctgccgg cgtggatcgg ctggctggtc ggcgacgccg tcggcgcgct
      121 gctggccggc gtgccgctga tcgcctggag ccgcgcgagc gcggcgctgg cgttcggggg
      181 ccccgccggg ctgagcaacc tgctcttgca ggcggtggtg gtggcttgtg gcctgaccgc
      241 cttttccggc tggctggatc gcgactccgc gttcgtctac ccgctgctgg cgctgccgtt
      301 cttcctgctg gcgctgctgg cgatgcgcgg gggcgtcatc gcggcgtcga tcggcgtgct
      361 gttgctgtcg atcgccaccg gctacggcac cgcgcgcggc gtcggcccct tcgcgctgca
      421 cgacccgcgc gccggttcgc tggcgctgtg gagctacgtc accgcgcagg cctgcaccag
      481 cctgctgatc tgcagcctgg gcatggcgct gcaggccagc cagcgccagt tcgaggcctt
      541 cctgcagaac gtctccgacg gcatcctggt gatcgacgac cagggcgtgc tgctgcatgc
      601 caacgcggcg ttcacggcga tgaccggcac cgatgccgcg acgctggtgg gccggcgcgc
      661 ggccgacgcg atgcgcggtg gcgcgggcgc gatcgcggcg ctggtggccg gtggcgcgcc
      721 ggggtcgacc accgaactga cgctgccgcg cgcgggcggc gggcggcggg tggtcgagtg
      781 ccaggtggcg cgctaccgca aggcgagcgg gtactggcag acgcacgtca cgctgcgcga
      841 catcacgcag cgcgtcgagg cgcaggagca gctggccacc agcgaggcgc ggctcaaggc
      901 cgtcaccgac aacgtgccgg cgatgttcgc gtacgtcgac cgggaccagg tctaccgctt
      961 cgccaacggc cacttccgcc acgtgatggg cgccgagccc gagtccgtga tcggccgcac
     1021 gatgcgccag ttcctgggcg atgcggcgca cgccgagctg ctgccgcaca tcgagggcgc
     1081 gctgcgcggc gagccgcagc agttcgagcg caccggctgg cgcgagaacg cgaaccggca
     1141 cttcctggcg cagtacgtgc cggaggtgcg gcccgacggc agcgtgcccg gcttcttcat
     1201 catggtgctc gacatcaccg agcgcttccg cgccgagctc gcgctcgcgc gcagcgaggc
     1261 gctggtgcgc gccatcgccg accacatgcc gggcctgatc tcgcgcatgg accgcgacta
     1321 ccgctacacc ttcgtcaacg ccaactacat gagctggttc tcgctgcccg agtccccggt
     1381 gggcaagacg gtggccgagg tcttcggcca ggatgtcttc gagggcgtgc acgcgcgaat
     1441 cgaccaggcg ctggcgggcc acgacgtcgt cttcgacctg accaacaagg tgcccggcgg
     1501 cgtcgacttc atgcaggtgc actacgtgcc tgaccgcgac gagcgcgggc aggtgatcgg
     1561 cgtgtacggc ctggtcaccg accgcaccga gcagcagcac gcgcgcgacc gcatcgaggc
     1621 cagcgagcgg cagttgcgcg ccgtcaccga caacctgccg atgctgatca cctacgtcga
     1681 cgccgacgag cgcctgcgct tcatgaacgc gaccttccac gtgtggctgg gcaccgacat
     1741 cgccgccgcg atcggccggc cgctgcgcga cgtggtcggc gaggagcact acgccgcgcg
     1801 gcgcgatcac ctgcgcgcgg cgctcgccgg ccggcgcgtc gagttcgagg tcgtctcgcg
     1861 caccctgcag gggctgcgca acctgcagac ggtctacatc cccgacgtcc gcgacgacgg
     1921 cagcgtgcgc ggcatcttca cgatgagcac cgacgtcacc tcgctgaagg agaccgagcg
     1981 cgagctgcag cgcctcgcgc gcgtggacac gctcacgggc ctggccaacc ggcgccagtt
     2041 cgacgagctg ctggaacagt cgctggcgcg ctaccgccgc gcgcgccggc cgctcgcgct
     2101 gatctacctg gac
//
LOCUS       DATCRO010000553         9806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_535132, whole genome shotgun sequence.
ACCESSION   DATCRO010000553 DATCRO010000000
VERSION     DATCRO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535132"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..2124
                     /locus_tag="VIN75_15070"
     CDS             235..2124
                     /locus_tag="VIN75_15070"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEY8975538.1"
                     /translation="MRINMLLTAALCASGLVAAQSALAAPLKALAHADSQATVAAAAS
                     QDVVEFGIYLPLRHKDQLEALIARQHDEHSPQYHQWLQPAEFLAKYGPRPADIEAVKD
                     ALTARGLTIVSSNAHGVRARGTAAQVSQAFSAPLEKVTRHSHTRFQARSGLRIPGELA
                     GVDARVFGLATVPERHIHAHVLGKAPEANSPDNRYSTTGPYWFTDLKQAYDYPAWSAK
                     TDGTGVTVAVLMADLIFPNDVAAMFNHEKFTAVTGQAVPTVDTVTIDGGGVYDGYGSF
                     EASLDVQQVLGGAPGSKVTLVSIPDLSDQHIMDGYQYIIDSNQYDIVNSSFGGCELAY
                     TAAYNGGVDYTDLLQAQHEMFLQGNAQGITFVASSGDESGLECPSADYVTGGTAPTFV
                     PSVSTPAADPAVTAVGGGNLVTNSAPPSLDSTYVRESAYNDPEVPSDPWGLGVNVSGG
                     AWGAGGGVSVVFRKPLYQYLANTGTNHWRTIPDVGMQVGGCPGSAISCNDDDSAAVVS
                     YGVGIGGGNYGVIGTSVSSPEFVGALALFEQQLGKKNHRMGNANYYLYAMGALQTLAG
                     GTKAPAALQFYHRNNPGFNGVANDSFPSYNYNYVYGNGSPDVRKLFGLTQYKAAGVPQ
                     TTTNP"
     gene            2322..3029
                     /locus_tag="VIN75_15075"
     CDS             2322..3029
                     /locus_tag="VIN75_15075"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY8975539.1"
                     /translation="MTSTTSSRARAFQLALVASAAIGLGACSKTDSNDVPPPNAAASM
                     PSTVATAPTPAPAPAPAPLVVAQNDAANKSAYEAGRRDQIRQDARRAEQQRAARERAQ
                     ERQLPPRADRDTDVRNDQRVAAAACRECGVVESVTEVKVQGETNGVGALAGGATGALV
                     GNRIAGGNNRTLGGVVGAVGGGLIGNAIEKHHRQSIVYDVNVRMEDGSLRTVRESTAP
                     AIGEKVRVESDGLHQRT"
     gene            complement(3054..3884)
                     /gene="metF"
                     /locus_tag="VIN75_15080"
     CDS             complement(3054..3884)
                     /gene="metF"
                     /locus_tag="VIN75_15080"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY8975540.1"
                     /translation="MIKIPVSLEFFPPKTPEGMTKLAAARQQLYALDPEFCSVTYGAG
                     GSTQMGTLQVVQAIRDEGRVGVPHYTCIGATRASVGSTLDGFKDAGLKRLVALRGDLP
                     SGSGSFGEFRYATELIEAIRKHSDDHFHIEVAAYPETHPQARSPEADLKAFAAKAAAG
                     ANSAITQMFFNADAYFRFVDDARAMGVTIPIVPGIMPITNSSGLMRFADNTGCEIPRW
                     MRLRLQSFGDDTASIKAFGLDVMARLCERLVAGGVPALHFYTMNQSAATLALVDAIGG
                     "
     gene            complement(4135..5562)
                     /gene="ahcY"
                     /locus_tag="VIN75_15085"
     CDS             complement(4135..5562)
                     /gene="ahcY"
                     /locus_tag="VIN75_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8975541.1"
                     /translation="MNAALTPLPTDAYAVADLKLADWGRKEIAIAESEMPALMAMRAE
                     YGSSLKGARIAGSLHMTIQTAVLVETLQALGAQVRWASCNIFSTQDHAAAALVAQGTP
                     VFAYKGETLKDYWDYTHRIFEFGAKGDKDEGPNMILDDGGDATLLMHLGLRAEKDLSV
                     VGNPTSEEERELFAAIKAKLAVDPTWYTRKSAQIIGVTEETTTGVHRLNEMSAKGTLL
                     FRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVAGYGDVGKGSAQAL
                     RALSAQVWVTEIDPINALQAAMEGYRVVTMDDAAAHGDIFVTCTGNKNVITYEHMAAM
                     KEQSIVCNIGHFDNEIDIASLEHCKWEEIKPQVDHVIFPNGKRIIMLAKGRLVNLGCG
                     TGHPSYVMSSSFANQTIAQVELFTRPDAYQKGKVYVLPKHLDEKVARLQLSKLNAKLT
                     ELTDEQAAYIGVSKAGPYKPDSYRY"
     regulatory      complement(5651..5741)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(5792..6634)
                     /locus_tag="VIN75_15090"
     CDS             complement(5792..6634)
                     /locus_tag="VIN75_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8975542.1"
                     /translation="MEGSNFSRPAGRNGSSDTGRIATARPNDVAPSASMAPALSSGIA
                     GIALAALEAELAYRGPERRSGPGAGLAKLLASVLDEVDYGLVLIAADGHVVHANHAAR
                     VELAGAKTLQLVGRRLSGRSAVDQRALDEALASARDEGKRRMLAFAPANALSEGKSCD
                     LSIVPLPAPLSSNHSGHAVLISLPRSRIAETLSVDAYARELGLSRREQQVLAGLCEGL
                     RVKEIAAKLEIGDETVRSHVKRLKAKTGCAGIVDIVNQVSRLPPMVGALRQVADLPPI
                     DERS"
     gene            complement(6797..7945)
                     /locus_tag="VIN75_15095"
     CDS             complement(6797..7945)
                     /locus_tag="VIN75_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8975543.1"
                     /translation="MPRTKTITLDHLRRYAVARTLFPPTTLQKAIERLGFVQADPIRA
                     PARAQDLTLRHRVKDYRAGDLERRYPRLAVEEDCLVNYGFVPRSLLPLMHPRTPARGW
                     DAAMQRRADEVLAFVRARGATHPRDVQEVFDHGRVQSWGATNTLNASTALLDGLHYRG
                     LLRVKRRENGTRVYEAVDHPPADDDPAARHERARRLLMTIVEKYAPLPSASLGYLARL
                     LEYGAPHLHGETRTLLAELRAGLPQARIDGVTWLWTPGEDPASRKWRVDDGRARLLAP
                     FDPVVWDRRRFELLWGWTYKFEAYTPAVKRTMGHYALPLLYGDRAIGWANATLVDKAR
                     LEVHVGFADKAWAREKALAAAIDDELAGMAEFLQLGRGAATRVKRPRK"
     gene            8038..8949
                     /locus_tag="VIN75_15100"
     CDS             8038..8949
                     /locus_tag="VIN75_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8975544.1"
                     /translation="MPTASRTPPTPPSPQAVTWDWRVLAAGALAIAAGLWLRSNAACN
                     EALFHAVNRFGPDAPAAWSMLSVAGLALSAFIYLTACADEVPERVAQLLWGIVLGGLT
                     ASWIKHHLPSPRPFLALGAEHLAVVGTPLGSGSMPSGHSAMAFAMLAVLVAERRRFGE
                     RGAVDGVLTSNLGIVLIALLALGIALSRLAVGAHWPADTLVGGGLGLVFGGLAPHAWP
                     VGAMTRLLSRPLGQRLMAAGLLLCAFCIAATPGLLDATGLVDRKWARNLLPGYPLAAT
                     LQYVLALVALAGAARWWRASGRAAVAD"
     gene            8954..>9806
                     /locus_tag="VIN75_15105"
     CDS             8954..>9806
                     /locus_tag="VIN75_15105"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY8975545.1"
                     /translation="MTRTRWIAAASIAAGVALLAVLVVFTPWRLLAERVATVPWPGWV
                     GATLGMSATYALRAGRLRAEWLWKLRTLGLGYRECLQVTLLHNAAINILPMRSGEASY
                     SFLLHRRWGVGLGDATASLLWLRLQDMMVLGVLGIAILVPAPLPWRIGFAAAAILAAA
                     TLLPVLVRRIHVHARWSRARAHASARTRRAWHLVAKVAAAFRAARGGAAAWGFAIANW
                     MLKLGIVGVLLASLADLPPGAGFCGALGGELAGVLPLQAPAGVGTYEAGVALGTRARV
                     RESPASAP"
BASE COUNT         1336 a         3539 c         3456 g         1475 t
ORIGIN      
        1 gtcgcgagtc gatcgccaca ggcggcggcc gatcggccgt ccaggctgcc ggcggcggcg
       61 cacgggatcc cccatttcgg tgagcgccgc gccgaatacc tgtcgatccg cgcaaacccg
      121 catcgggata cctcgccccg cggtccgggg gttcgcgtcg ttgacacacc aacgcgccga
      181 accgagactt ttttcctcgg aaatccactt tcttccaccg tgaggggaac ccccatgcga
      241 atcaacatgc tattgacggc cgccttgtgc gccagcggcc tcgtcgccgc ccagtccgcg
      301 ctcgcggcgc ccctgaaggc gctggcccac gcggactcgc aggcgacggt cgccgccgcc
      361 gcgtcgcagg acgtcgtcga gttcggcatc tacctgccgt tgcgccacaa ggaccagctc
      421 gaggccctga tcgcgcgcca gcacgacgag cattcgccgc agtaccacca gtggctgcag
      481 ccggcggaat tccttgccaa gtacggcccg cgtccggccg acatcgaagc cgtcaaggac
      541 gcgctgaccg cgcgcggcct gaccatcgtg tccagcaacg cccacggcgt gcgtgcgcgc
      601 ggcaccgcgg cccaggtgtc gcaggccttc agcgcgccgc tggagaaggt cacccggcac
      661 tcgcacaccc gcttccaggc ccgcagcggc ctgcgcatcc cgggcgaact ggccggcgtc
      721 gacgcgcgcg tgttcggcct ggccaccgtg ccggagcgcc acatccacgc ccacgtgctc
      781 ggcaaggccc ccgaggccaa cagcccggac aatcgctaca gcaccaccgg cccgtactgg
      841 ttcaccgacc tgaagcaggc ctacgactac ccggcctgga gcgccaagac cgacggcacc
      901 ggcgtcaccg tggcggtgct gatggccgac ctgatcttcc cgaacgacgt ggccgcgatg
      961 ttcaaccacg agaagttcac cgcggtgacc ggccaggccg ttcccacggt cgacaccgtg
     1021 accatcgacg gcggcggcgt ctacgacggc tacggctcgt tcgaggccag cctcgacgtg
     1081 cagcaggtgc tcggcggcgc gcccggctcc aaggtcacgc tggtgagcat ccccgacctg
     1141 tccgaccagc acatcatgga cgggtaccag tacatcatcg acagcaacca gtacgacatc
     1201 gtgaactcgt cgttcggcgg ctgcgagctc gcgtacaccg cggcgtacaa cggcggcgtc
     1261 gactacaccg acctgctgca ggcccagcac gagatgttcc tgcagggcaa cgcccagggc
     1321 atcacgttcg tggccagctc gggcgacgag tcgggcctgg agtgcccgag cgccgactac
     1381 gtcaccggcg gcaccgcgcc gaccttcgtg cccagcgtca gcaccccggc ggcggacccg
     1441 gccgtgacgg cggtcggcgg cggcaacctg gtcaccaaca gcgcgccccc gtcgctggac
     1501 tcgacctacg tgcgcgagag cgcctacaac gacccggaag tgccgagcga cccgtggggc
     1561 ctgggcgtga acgtgtccgg cggcgcctgg ggcgcgggcg gcggcgtcag cgtcgtgttc
     1621 cgcaagccgc tgtaccagta cctggccaac accggcacca accactggcg caccatcccc
     1681 gacgtgggca tgcaggtcgg cggctgcccg ggctcggcca tcagctgcaa tgacgacgac
     1741 agcgcggcgg tggtgtcgta cggcgtgggc atcggcggcg gcaactacgg cgtgatcggc
     1801 acctcggtgt cctcgcccga gttcgtcggc gcgctggcgc tgttcgagca gcaactcggc
     1861 aagaagaacc accggatggg caacgccaac tactacctgt acgcgatggg cgcgctgcag
     1921 acgctggccg gcggcaccaa ggccccggcc gcgctgcagt tctaccatcg caacaacccg
     1981 ggcttcaacg gcgtcgccaa cgacagcttc ccgagctaca actacaacta tgtctacggc
     2041 aacggcagcc ccgacgtgcg caagctcttc ggcctgacgc agtacaaggc ggccggcgtc
     2101 ccgcagacga cgaccaatcc gtaagccgac ctgtatccag gtgcaagggg cgttcgccac
     2161 gcggcgaacg cccttttttc atgggcagcg ccgacgcgtg cgcggcggcc agctcgaccg
     2221 cgatgcatcc ggatgtttcc atggccgggc acgcgggcat gcgcatcgcc acctgaccgg
     2281 aatgacgggc ccctccggcc cgaccggaaa aggagactcc catgaccagc accacctcgt
     2341 cccgcgcccg cgccttccag ctcgcgctcg tcgcctcggc ggcgatcggc ctgggcgcct
     2401 gttcgaagac cgattcgaac gacgtgccgc cgcccaacgc ggccgcgagc atgccgtcca
     2461 ccgtcgcgac ggcgcccacg cctgccccgg cgccggcgcc tgccccgctc gtcgtcgcgc
     2521 agaacgacgc ggccaacaag agcgcctacg aggcgggccg gcgggaccag atccgccagg
     2581 acgcgcgccg cgccgagcag caacgcgccg cgcgcgagcg cgcgcaggag cgccagctgc
     2641 cgccgcgcgc cgaccgcgac accgacgtgc gcaacgacca gcgcgtggcc gcggccgcct
     2701 gccgcgaatg cggcgtggtc gagtccgtga ccgaggtgaa ggtgcagggc gagaccaacg
     2761 gtgtcggcgc gctggccggc ggcgccacgg gcgcgctggt gggcaaccgc atcgccggcg
     2821 gcaacaaccg cacgctgggc ggcgtcgtcg gcgcggtcgg cggcggcctg atcggcaacg
     2881 ccatcgagaa gcaccaccgc cagtccatcg tctacgacgt caacgtgcgc atggaggacg
     2941 gcagcctgcg cacggtgcgc gagtcgacgg cgccggcgat cggcgagaag gtgcgcgtcg
     3001 agtcggacgg gctgcaccag cgcacgtaag cacgtgcgcc acgcgatcgg cgttcagccg
     3061 ccgatcgcgt ccaccagcgc cagcgtggcc gccgactggt tcatcgtgta gaagtgcagc
     3121 gccggcacgc ccccggcgac caggcgctcg cacaggcgcg ccatcacgtc caggccgaag
     3181 gccttgatcg atgcggtgtc gtcgccgaag gactgcagcc gcagccgcat ccagcgcggg
     3241 atctcgcagc cggtgttgtc ggcaaagcgc atcaggccgc tggagttcgt gatgggcatg
     3301 atgcccggca cgatggggat cgtgacgccc atcgcgcgcg cgtcgtcgac gaagcggaag
     3361 tatgcgtcgg cgttgaagaa catctgcgtg atggccgagt tcgcgccggc ggcggccttg
     3421 gccgcgaagg ccttcaggtc ggcctcgggc gagcgcgcct gcggatgcgt ctcgggatag
     3481 gccgccacct cgatgtggaa gtggtcgtcc gagtgcttgc ggatcgcctc gatgagctcg
     3541 gtggcatagc ggaactcgcc gaagctgccc gagccgctgg gcaggtcgcc gcgcagcgcg
     3601 acgaggcgct tgagccccgc gtccttgaag ccgtccagcg tcgagcccac gctcgcgcgc
     3661 gtggcgccga tgcaggtgta gtgcggcacg cccacgcggc cctcgtcgcg gatcgcctgc
     3721 accacctgca gcgtgcccat ctgcgtggac ccgccggcgc cgtaggtgac cgagcagaac
     3781 tcgggatcga gcgcgtacag ctgctgccgc gccgcggcca gcttggtcat gccttcgggg
     3841 gtcttcggcg ggaagaactc gaggctgacg gggatcttga tcatggttgt tccagtgaaa
     3901 tggcttcgcg tccaggaacc ggccggaggg gtctggccgg aggggtctgg cctctcctcg
     3961 aaatcgcgcc gcgacgccgc cgcaagccga tgaggcggcc gcgattcgag gcgaaggcct
     4021 gaccccggtc cccggctgac gttcggggtc aggccctcgc ctcctgtcgc cggtggcatg
     4081 tctgccgatg gctgcgccct ggcggcgaca tgaggagagg ccagacccct ttgctcagta
     4141 gcggtacgag tcgggcttgt acggccccgc cttcgacacg ccgatgtacg ccgcctgctc
     4201 gtcggtcagc tccgtcagct tggcgttgag cttggacagc tgcaggcgcg ccaccttctc
     4261 gtccaggtgc ttgggcagca cgtagacctt gcccttctgg tacgcgtcgg ggcgcgtgaa
     4321 cagctcgacc tgcgcgatcg tctggttggc gaacgacgac gacatcacgt agctcggatg
     4381 gcccgtgccg cagcccaggt tcacgaggcg gcccttggcc agcatgatga tgcgcttgcc
     4441 gttcgggaag atcacgtggt cgacctgcgg cttgatctcc tcccacttgc agtgctcgag
     4501 cgaggcgatg tcgatctcgt tgtcgaagtg gccgatgttg cagacgatcg actgctcctt
     4561 catcgcggcc atgtgctcgt acgtgatgac gttcttgttg ccggtgcagg tgacgaagat
     4621 gtcgccgtgc gcggccgcgt cgtccatcgt caccacgcgg tagccttcca tcgcggcctg
     4681 cagcgcgttg atcgggtcga tctcggtcac ccagacctgc gccgacagcg cgcgcagcgc
     4741 ctgcgccgag cccttgccca cgtcgccgta gccggccacg acggcgatct tgccggcgac
     4801 catcacgtcg gtcgcgcgct tgatgccgtc cacgagcgac tcgcggcagc cgtacaggtt
     4861 gtcgaacttg ctcttggtga cggagtcgtt gacgttgatg gcgcggaaca gcagcgtgcc
     4921 cttggccgac atctcgttga ggcggtgcac gccggtcgtc gtctcttcgg tcacgccgat
     4981 gatctgggcg ctcttgcgcg tgtaccaggt cgggtcgacg gccagcttgg ccttgatcgc
     5041 ggcgaacagc tcgcgctctt cctcgctggt cgggttgccg acgacggaga ggtccttctc
     5101 ggcgcgcagg cccaggtgca tcagcagcgt cgcgtcgccg ccgtcgtcca ggatcatgtt
     5161 cgggccttcg tccttgtcgc ccttggcgcc gaactcgaag atgcggtggg tgtagtccca
     5221 gtagtccttg agcgtctcgc ccttgtacgc gaacaccggc gtgccctgcg ccacgagcgc
     5281 ggcggcggcg tggtcctgcg tggagaagat gttgcacgac gcccagcgca cctgcgcgcc
     5341 gagcgcctgc agggtctcga ccagcacggc cgtctggatc gtcatgtgca ggctgccggc
     5401 gatgcgggcg cccttgagcg agctgccgta ctcggcgcgc atggccatca gcgcgggcat
     5461 ctcgctctcg gcgatggcga tttccttgcg gccccagtcg gccagcttca ggtcggcgac
     5521 ggcgtacgcg tcggtcggaa ggggggtcag tgctgcgttc attcgggttc tccgttgagt
     5581 acccgccacg cgccgcgaag gccgcgggga aggcgacctt gcaccttcac ccggaacctc
     5641 gcgcgagcga agcgggtgag cgcggttgcc tttgatgggg ttccgagcct ggggcgcgca
     5701 gccgtgatcg acggccggac ctcgcaacgc tcctcggaat ggacgagatt atatcggccg
     5761 cctcccggcg gcctcaagcc tcgtcagcga ttcaagaccg ttcgtcgatc ggcggcaggt
     5821 cggccacctg gcgcagcgcg ccgaccatgg gcggcaggcg cgagacctgg ttgacgatgt
     5881 cgacgatgcc ggcgcagccc gtcttggcct tgagccgctt gacgtggctg cgaacggtct
     5941 cgtcgccgat ctcgagcttg gcggcgatct ccttgacgcg caggccctcg cacaggccgg
     6001 ccagcacctg ctgctcgcgg cgcgacaggc ccagctcgcg ggcataggcg tcgaccgaca
     6061 gcgtctcggc gatgcggctg cgcggcagcg agatcagcac cgcgtggccg gaatggttgg
     6121 acgacagcgg cgcgggcagc ggcacgatcg acaggtcgca ggacttgcct tcggacagcg
     6181 cgttggccgg cgcgaacgcc agcatgcggc gcttgccctc gtcgcgggcg ctggcgagcg
     6241 cctcgtcgag cgcccgctgg tcgacggccg agcggccgct caaccggcgg ccgaccagct
     6301 gcaaagtctt ggcgccggcc agctccacgc gcgcggcgtg gttggcgtgc acgacgtggc
     6361 cgtcggcggc gatcagcacg aggccgtagt cgacctcgtc cagcacgctg gcgagcagct
     6421 tggccaggcc ggcgcccggg ccgctgcggc gttcgggccc gcggtaggcc agctcggcct
     6481 ccagcgcggc cagcgcgatg ccggcgatgc ccgagctcag cgcgggcgcc atcgaggccg
     6541 acggcgcgac gtcgttcgga cgcgcggtcg cgatgcgacc ggtgtcggag ctcccgttgc
     6601 gtccggctgg acgactgaaa ttcgaccctt ccatgcgtgc ctcgcagagt attcaggact
     6661 gcttggccgg ccttgttccg tggaccgcgg cgagcgccgc gggcccggtg ggagccggca
     6721 attgcatttg acgtgctttt tgttacccgc gggacgccgg cgcctcgcgc aaccggccgt
     6781 ctcgtcaact tgtgcactac tttcgcgggc gcttgaccct ggtagcggca ccacggccca
     6841 gttgcaggaa ctccgccatc ccggcgagtt cgtcgtcgat cgcggcagca agcgctttct
     6901 cgcgcgccca ggccttgtcg gcgaagccca cgtgcacctc gagccgcgcc ttgtccacca
     6961 gcgtggcgtt ggcccagccg atcgcgcggt cgccgtacag caggggcagc gcatagtggc
     7021 ccatcgtccg cttgacggcc ggcgtgtagg cctcgaactt gtacgtccag ccccacaaca
     7081 gctcgaagcg gcggcgatcc cacaccaccg ggtcgaacgg cgcgagcagc cgcgcgcggc
     7141 cgtcgtcgac gcgccacttg cgcgacgccg gatcctcgcc cggcgtccac agccaggtga
     7201 cgccgtcgat gcgcgcctgc ggcagtccgg cgcgcagttc ggccagcagc gtgcgcgtct
     7261 cgccgtgcag gtgcggcgcg ccgtattcca gcagccgcgc aaggtagccc agcgaggccg
     7321 acggcagcgg cgcgtacttc tcgacgatcg tcatcagcag ccggcgcgca cgctcgtggc
     7381 gcgccgccgg gtcgtcatcc gccggcggat ggtcgacggc ctcgtagacg cgggtaccgt
     7441 tctcgcgccg cttgacgcgc agcaggccgc ggtagtgcaa gccgtccagc agcgcggtgc
     7501 tggcgttgag cgtgttggtc gcgccccagc tctgcacgcg gccgtggtcg aacacctcct
     7561 gcacgtcgcg cggatgcgtc gcgccgcgcg cccgcacgaa cgcgagcacc tcgtcggcgc
     7621 ggcgctgcat cgccgcgtcc cagccgcgcg cgggcgtgcg cggatgcatc agcggcagca
     7681 ggctgcgcgg cacgaagccg tagttgacga ggcagtcctc ctcgacggcc agccgcggat
     7741 agcggcgttc cagatcgccg gcgcggtagt ccttgacgcg atggcgcagc gtgaggtcct
     7801 gcgcccgcgc cggggcgcgg atcgggtcgg cctgcacgaa gcccaggcgc tcgatggcct
     7861 tctgcagcgt ggtcggcgga aacagcgtgc gggcgaccgc gtagcggcgc aggtggtcca
     7921 gcgtgatggt cttcgtgcgg ggcatcggaa gtggttcctg tcgagcgcat tctcgcgccg
     7981 gcccaggctc acccggcgag ccagggcgga ggcggccggt cgatacaatc gccgcccatg
     8041 ccgaccgcct cccgaacccc gccgacgccc ccttcgccgc aggcggtgac ctgggactgg
     8101 cgcgtgctcg ccgcgggcgc gctcgccatc gccgcgggcc tgtggctgcg ttcgaacgcc
     8161 gcctgcaacg aggcgctgtt ccacgccgtc aaccgcttcg gcccggacgc gcccgccgcc
     8221 tggtcgatgc tgagcgtcgc gggcctggcg ctgtcggcgt tcatctacct gacggcctgc
     8281 gccgacgagg tgcccgagcg cgtcgcgcag ctgctgtggg gcatcgtgct gggcggcctg
     8341 accgccagct ggatcaagca ccacctgcct tcgccgcggc ccttcctggc gctcggcgcg
     8401 gaacatctcg ctgtcgtggg aacacccctc ggttcaggct cgatgccctc gggccacagc
     8461 gcgatggcct tcgcgatgct ggccgtgctc gtggccgagc ggcgccgttt cggcgaacgc
     8521 ggcgcggtcg acggcgtgct cacgtccaac ctcggcatcg tgctcatcgc gctgctggcc
     8581 ctggggatcg cgctgtctcg gctggccgtc ggcgcgcact ggccggcgga cacgctcgtc
     8641 ggcggcggcc tgggcctcgt gttcggcggc ctcgcgccgc acgcctggcc ggtgggcgcg
     8701 atgacgcggc tgctctcgcg gcccctgggc cagcggctga tggccgctgg cctcctgctg
     8761 tgcgccttct gcatcgccgc cacccccggc ctgctggatg ccaccggcct cgtcgacagg
     8821 aaatgggcgc gcaacctgct gcccggctat ccgctggcgg ccacgctgca atacgtcctg
     8881 gccctcgtcg cgctcgcggg cgccgcgcgc tggtggcgcg cgagcggccg cgccgccgtc
     8941 gctgattgac ccgatgaccc gcacccgctg gatcgccgcc gccagcatcg ccgccggcgt
     9001 cgcgctgctg gccgtgctgg tggtcttcac gccgtggcgg ctgctggccg agcgcgtggc
     9061 caccgtgccg tggccgggct gggtcggcgc cacgctgggc atgagcgcca cgtacgcgct
     9121 gcgcgccggc cggctgcgcg ccgagtggct gtggaagctg cgcaccctcg gcctgggcta
     9181 tcgcgagtgc ctgcaggtca cgctcctgca caacgcggcc atcaacatcc tgccgatgcg
     9241 ctcgggcgag gcgagctact cgttcctgct gcaccggcgc tggggcgtag gcctgggcga
     9301 cgccacggcg agcctgctgt ggctgcggct gcaggacatg atggtgctgg gcgtgctggg
     9361 catcgcgatc ctggtgccgg cgccgctgcc gtggcgcatc ggcttcgcgg cggccgcgat
     9421 cctggcggcg gccacgctgc tgcccgtgct ggtgcgccgc atccacgtgc atgcccgctg
     9481 gtcgcgcgcg cgggcccatg cgtccgcccg cacgcgcagg gcctggcacc tggtggccaa
     9541 ggtcgcggcc gcgttccgcg ccgcgcgcgg cggcgcggcg gcctggggct tcgcgatcgc
     9601 caactggatg ctcaagctgg gcatcgtcgg cgtgctgctc gcctcgctgg ccgacctgcc
     9661 gccgggcgcg ggcttttgcg gcgcacttgg cggcgagctc gcgggcgtgc tgccgctgca
     9721 ggcgcccgcc ggcgtcggca cgtacgaggc cggcgtcgcc ctcggcacgc gggcccgcgt
     9781 gcgcgaatcg ccggcatcgg cgccgg
//
LOCUS       DATCRO010000554         8737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_658892, whole genome shotgun sequence.
ACCESSION   DATCRO010000554 DATCRO010000000
VERSION     DATCRO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658892"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="yidC"
                     /locus_tag="VIN75_15110"
     CDS             <1..790
                     /gene="yidC"
                     /locus_tag="VIN75_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY8975546.1"
                     /translation="PVGTVAPGATQSVKATLFAGPQEEKLLAPLAPNLEQVKDYGRLK
                     IIAQPLFWLLSKIHTVIGNWGWSIVALVVLLKIAFYGLNANAYRSMAKMKAVNPRIQE
                     LNVRYKDNPQQKQQEIMKIYREEKVNPLGGCLPILIQMPIFIALYTVLTSSAEMRGAP
                     WMGWIHDLSIMDPYAILPILMTATSLIQVSLQPTPADPVQAKMMWLMPLAFSAALFSF
                     PAGLTLYWVTNNALTILQQWMINRGLEGEKAKGLKAADAKIVKG"
     gene            870..2258
                     /gene="mnmE"
                     /locus_tag="VIN75_15115"
     CDS             870..2258
                     /gene="mnmE"
                     /locus_tag="VIN75_15115"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY8975547.1"
                     /translation="MNAATDLAHLTDPIVAIATAPGRGAVGIVRASGKDLSTLVAALC
                     AKPLTPRVAHYGPILDAQGQALDQGLAIHFPTPNSYTGEDVLELQAHGGPVVLQLILA
                     RCLEAGRGIGLRLANPGEFTARAFFNDKLDLAQAEAVADLIDASTEAAARSAGRSLAG
                     AFSREITALAERIVHLRMLVEATLDFPEEEIDFLEKAGARAQLADIRARLAAVMARAR
                     QGALLREGLRVVLAGAPNVGKSSLLNALAGAELAIVTPIAGTTRDRITETIQIHGVPI
                     HMIDTAGLREASDEVERIGIERSWQAIADADAVLFLHDLSRADEPAAQADDVAIADAL
                     ARAHAAEPLHVYNKRDLAPGHALPDGALALSAQTGAGLDALRDALLERAGATHVPDGV
                     FIARTRHVQALARTDDHLQLAQSHADQRDAALDLFAEELRLAHGALGEITGQFTADDL
                     LGEIFGRFCIGK"
     gene            2635..3102
                     /locus_tag="VIN75_15120"
     CDS             2635..3102
                     /locus_tag="VIN75_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8975548.1"
                     /translation="MNVLNLVQSIQTLNAYDAFRPRFSPENWRVVGSYLIRREMKGGD
                     VLIYQGDSDRTMYLLESGTLQVYVRGTATSAAKIALLRAGSIVGEPALFGETMRMAQV
                     DAIAPSVVWALSRPRLDEMLAGQPELAYEILRASGAVMAERMRGTLERGTPVA"
     gene            complement(3117..5117)
                     /locus_tag="VIN75_15125"
     CDS             complement(3117..5117)
                     /locus_tag="VIN75_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8975549.1"
                     /translation="MASTLELVLLYLAAAIAGVMGFRMLRLPPVLGYLVAGVIIGPNA
                     LQLAHDTAGVHSLAEFGVVFLMFVIGLEFNLPKLRAMRTLVFGLGASQVVLTILGAMA
                     GNFAIIWICSLIGRHWDLTWQAAAILGSAMAMSSTALVVKLMAERLELESEHGRRVMG
                     VLLFQDLAVVPLLVIIPALGGDTRDLFKTLVPAILKAVVVLAVLLSGGQKVMKWFLTA
                     ISRRKSDELFMLALLGVTLGLAAVTEMAGLSLALGAFVAGMLIAETEYKHQVETDIRP
                     FHDVLLGLFFITIGMKLDWHVLADEWVAVIVLTTVPILVKASLVAGLARVFGAAAGVA
                     LRTGLYLAQAGEFGFVLLTLGTDNHVLPDRWVAPVLASMVLSMLASPFVILYANRIVM
                     KLASSDWLMQSVALTTIAKRAITAEKHVIICGYGRSGQNLARMLEVERIPFMALDLDP
                     DRVRQAAAAGQNVVFGDAARLSSLMAAGLARAAAVVCSYHDTPSALKVLHLVQTHAPR
                     VPVIVRTIDDSDLEVLKAAGATEVVPEAIEGSLMLASHALALMGVPMRRVIRLTREAR
                     EARYGLLRGFFHGADDDEAGEQSQARLSSVTIPANAVAAGRTVAETGVIADGVQVVSL
                     RLSSGRVLPPNPEQRLAAGDTLVLAGLPEPLQAAEEKLTTGA"
     gene            5243..6229
                     /locus_tag="VIN75_15130"
     CDS             5243..6229
                     /locus_tag="VIN75_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY8975550.1"
                     /translation="MTNDLPFDAERALHLARQTFAIEADALLGMQRRLTAEFARAVET
                     ILRCEHRVVVMGMGKSGHVGRKIAATLASTGTPSFFVHPAEAAHGDLGMVTAGDVCLL
                     ISNSGESNELSAIIPALRRLGVTLVALTGRSDSSLAKVCDIVLSCAVDKEACTLNLAP
                     TASTTAQMAMGDALAVALLDSRGFREEDFALSHPGGALGRKLLMHVSDLMRSGDAVPA
                     VARDANFSTLLQVMSSKGLGMAAVVDGRTPVGIFTDGDLRRLIETGADLRQRVAHEVM
                     HPRPHLIRAGALAVDAADQMERHRITSLLVVDGKGELVGALNTYDLLHAKVI"
     gene            6229..6813
                     /locus_tag="VIN75_15135"
     CDS             6229..6813
                     /locus_tag="VIN75_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8975551.1"
                     /translation="MAGTPQSHLSFPPEILVKAQGAGAGIKAAIFDVDGVLTDGTIWI
                     TEAGETLKNFNVLDGQGLLMLRKGGITPIVITGRDSAAVRRRVSDLGLPHASFGASDK
                     VAAANDILQQLGVDWGDVAVIGDDWPDLPLFACAGFACAPANAHAEARGAAAYVTGRE
                     GGRGAAREFCDLLLMANGLYAPLMTKLLVTLDSK"
     gene            6821..7543
                     /gene="lptC"
                     /locus_tag="VIN75_15140"
     CDS             6821..7543
                     /gene="lptC"
                     /locus_tag="VIN75_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY8975552.1"
                     /translation="MKGELHIPDLPEVPVALSPLSPPVDDQQRRTRPSWPVRVRESLV
                     GYLPLMLMVGLALGTWLVAKNTPGLLSASGPAVVKHEPDYTLDQFNLQRFDPSGVLKV
                     EIQGVHMQHFPDDDIMEVEQIRVVTTEPDGRRMIATAQHGRARGDSTEVWLDGQAQVV
                     SEAPGELPIQMNGEHLHALPKVRKVDSKYMVVVRQGDSEFNADGLEYDDLTRLLTLHG
                     NVHALMQPALRKSMASTSDAKR"
     gene            7540..8316
                     /locus_tag="VIN75_15145"
     CDS             7540..8316
                     /locus_tag="VIN75_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975553.1"
                     /translation="MTAPLLFITGASSGIGQAMAVEWARRGGRLALVARRGDEMRAWA
                     ASQGWGADRAQVYVADARDLPAMTAAGRDCIARQGLPDVVIASAGISVGVDMGDEADL
                     EVLRAVVETNNLGMAATFQPFIKPMCERRAGRLVGIASVAGIRGLPGHAAYSMSKAGV
                     ISFCESLRVECRRFGVRVVTIAPGYIDTPLTRGNSYSMPFLMPADKFARQALDAIAAG
                     VGFRVIPWQMGVLARVLRILPDWIYDRAVAGRGRKARKSQ"
BASE COUNT         1361 a         3087 c         3044 g         1245 t
ORIGIN      
        1 gccggtgggc acggtcgccc cgggcgccac gcagtcggtc aaggccacgc tgttcgccgg
       61 cccgcaggaa gagaagctgc tggccccgct cgcgcccaac ctcgagcagg tcaaggacta
      121 cggccgcctg aagatcatcg cccagccgct gttctggctg ctgagcaaga tccacacggt
      181 gatcggcaac tggggctggt cgatcgtggc gctggtggtg ctgctcaaga tcgcgttcta
      241 cggcctgaac gccaacgcgt accgctcgat ggccaagatg aaggccgtca acccgcgcat
      301 ccaggagctg aacgtccgct acaaggacaa cccgcagcag aagcagcagg agatcatgaa
      361 gatctaccgc gaggagaagg tcaatcctct cggcggctgc ctgcccatcc tgatccagat
      421 gccgatcttc atcgcgctgt acacggtgct gacctcgtcg gccgagatgc gcggcgcacc
      481 ctggatgggc tggatccatg acctgtccat catggacccg tacgcgatcc tgccgatcct
      541 gatgacggcc acgtcgctga tccaggtctc gctgcagccc acgccggccg acccggtgca
      601 ggccaagatg atgtggctga tgccgctggc cttcagcgcc gcgctgttct cgttcccggc
      661 cggcctgacg ctgtactggg tcacgaacaa cgcgctgacg atcctgcagc agtggatgat
      721 caaccgcggc ctcgaaggcg agaaggccaa gggcctgaag gccgctgacg cgaagatcgt
      781 caagggctga gccctcccgc gtcacgacga cgccgcgttc ggccccgagc cgaccgcggc
      841 gtttttcatt tgacgacaat cgagccctga tgaacgccgc caccgacctc gcgcacctga
      901 ccgacccgat cgtcgccatc gccacggccc ccggccgcgg cgcggtgggc atcgtccggg
      961 cgtcgggcaa ggacctgtcg acgctggtcg ccgcgctgtg cgccaagccg ttgacgccgc
     1021 gcgtggcgca ctacggcccc atcctggacg cgcagggcca ggcgctcgac cagggcctgg
     1081 ccatccactt ccccacgccc aactcgtaca cgggcgagga cgtgctggaa ctgcaggcgc
     1141 acggcgggcc ggtggtgctg cagctgatcc tcgcgcgctg cctcgaggcg ggccgcggca
     1201 tcggcctgcg cctggccaat ccgggcgagt tcacggcgcg cgcgttcttc aacgacaagc
     1261 tggacctcgc gcaggccgag gccgtcgccg acctgatcga cgccagcacc gaggccgccg
     1321 cgcgatcggc cggacgttcg ctggcgggcg cgttctccag ggagatcacc gcgctggccg
     1381 agcgcatcgt ccacctgcgc atgctggtcg aggcgacgct ggatttcccc gaggaggaga
     1441 tcgacttcct cgagaaggcc ggcgcgcgcg cgcaactcgc cgacatccgc gcgcggctcg
     1501 cggccgtcat ggcccgtgcg cgccagggcg cgctgctgcg cgagggcctg cgcgtggtgc
     1561 tcgccggggc gcccaacgtc ggcaagagct cgttgctgaa cgcgctggcc ggcgcggagc
     1621 tggccatcgt cacgccgatc gccggcacca cgcgcgaccg catcaccgag acgatccaga
     1681 tccacggcgt gccgatccac atgatcgaca ccgccggact gcgcgaggcg agcgacgagg
     1741 tcgagcgcat cggcatcgag cgcagctggc aggcgatcgc cgatgccgac gcggtgctgt
     1801 tcctgcacga cctgagccgc gccgacgagc ccgccgcgca ggccgacgac gtggcgatcg
     1861 ccgacgccct tgcacgcgcg cacgcggccg agccgctgca cgtctacaac aagcgcgacc
     1921 tcgcgcccgg tcacgccttg cccgatggcg cgctggcctt gtccgcgcag accggcgccg
     1981 gcctcgacgc gttgcgtgac gccctgctcg aacgcgccgg cgccacccac gtccccgacg
     2041 gcgtgttcat cgcgcgcacg cgtcacgtgc aggcgctcgc gcgcacggac gaccacctgc
     2101 agctcgcgca gtcgcacgcc gaccagcgcg acgccgcgct cgacctgttt gccgaggaac
     2161 tgcgcctggc gcacggcgcg ctcggcgaga tcacgggcca gttcacggcc gacgacctgc
     2221 tcggggagat cttcgggcgg ttctgcatcg ggaaatgatc gcgggcgcgg gtaccgtggg
     2281 gagcgcgccg atcgcgctgc gcggagtgtg tgatcgctcc ggcacccctc ggtcaggctg
     2341 atggcgacgt gaccgaatgc gcgaatagag tcgcctcccg ataccgcaga tcggggatgg
     2401 catcatcgtt gaagtgagcg cctgccgtcg gccagcgcgg caggatgcgt gtttcgtggg
     2461 ccgctggcgt ccggaagatg cgacagacgg catgctggcg ccgacggacg tcgcgctttg
     2521 ccttgccgcc ggcggcttcc ggacgtgaca attggtgtca aatgcctgtc ccagggaacg
     2581 atgggttgca acgccgtgcg gcgcttgccg ggagccatcg caaggaccaa gagaatgaac
     2641 gttttgaatc tggtgcagtc catccaaacg ctgaacgcct atgacgcgtt ccgtccgcgc
     2701 ttttcgccgg agaactggcg tgtggtgggc tcgtacttga tccgccgcga gatgaagggc
     2761 ggcgacgtcc tgatctacca gggcgactcc gaccgcacga tgtacctgct ggagtcgggc
     2821 acgctgcagg tgtacgtccg cggcaccgcc acgtcggccg ccaagatcgc gctgctgcgc
     2881 gccggctcca tcgtcggcga gccggccctg ttcggcgaga cgatgcgcat ggcgcaggtc
     2941 gacgcgatcg cgccgagcgt cgtctgggcg ctgagccgtc cgcggctgga cgagatgctg
     3001 gccggccagc ccgagctcgc gtacgagatc ctgcgcgcct cgggcgcggt catggccgag
     3061 cgcatgcgcg gcaccctcga gcgcggcacc cccgtcgcct gacgccgtcc ggcggctcag
     3121 gccccggtcg tcaacttctc ttcggccgcc tgcagcggct ccggcaatcc cgccagcacc
     3181 agcgtgtcgc ccgcggccag gcgctgctcg ggattgggcg gcagcacgcg gccgctggac
     3241 agccgcagcg acaccacctg cacgccgtcg gcgatgacgc ccgtctcggc gacggtgcgt
     3301 ccggcggcaa ccgcgttggc ggggatggtg acgctggaca gccgcgcctg cgactgctcg
     3361 ccggcctcgt cgtcgtcggc gccgtggaag aagccgcgca acaggccgta gcgcgcctcg
     3421 cgggcctcgc gcgtgagccg gatcacgcgc cgcatcggca cgcccatcag cgccagcgcg
     3481 tgcgaggcca gcatcaggga gccctcgatc gcctcgggca cgacctcggt cgcgccggcg
     3541 gccttgagca cctcgaggtc ggagtcgtcg atcgtgcgca caatcacggg cacgcgcggc
     3601 gcgtgcgtct ggaccaggtg cagcaccttc agcgcggatg gggtgtcgtg gtagctgcag
     3661 accaccgccg ccgcgcgcgc gagccccgcg gccatcaggc tggacagccg cgccgcgtcg
     3721 ccgaacacca cgttctggcc ggcggccgcg gcctggcgta cgcggtcggg gtccaggtcc
     3781 agcgccatga acgggatgcg ctcgacctcc agcatgcgcg ccaggttctg gccggagcgg
     3841 ccgtagccgc agatgatcac gtgcttctcg gccgtgatgg cgcgcttggc gatcgtcgtc
     3901 agcgccaccg actgcatcag ccagtcgctc gaggccagct tcatgacgat gcggttggca
     3961 tagaggatga cgaacggcga cgcgagcatc gacagcacca tgctggccag caccggcgcc
     4021 acccagcgat cgggcagcac gtggttgtcg gtgcccagcg tcagcagcac gaagccgaac
     4081 tcgccggcct gcgcgaggta caggccggtg cgcagcgcga cgcccgcggc ggcgccgaac
     4141 acgcgcgcca ggccggccac gaggctcgcc ttgacgagga tgggcaccgt ggtcagcacg
     4201 atgaccgcca cccattcgtc ggccagcacg tgccagtcga gcttcatgcc gatcgtgatg
     4261 aagaacaggc ccagcagcac gtcatggaac ggccggatgt cggtctccac ctggtgcttg
     4321 tattcggttt ccgcgatcag catgccggcg acgaacgcgc ccagcgcgag cgacaggccg
     4381 gccatctcgg tgaccgccgc caggcccagc gtgacgccca gcagcgccag catgaacagc
     4441 tcgtcgctct tgcggcgtga gatggcggtc aggaaccact tcatcacctt ctggccgccg
     4501 gacagcagca ccgccagcac caccaccgcc ttgaggatgg ccggcaccag cgtcttgaac
     4561 aggtcgcgcg tgtcgccgcc cagcgccggg atgatcacca gcaacggcac cacggccagg
     4621 tcctggaaca gcagcacccc catcacgcgg cggccgtgct cgctctcgag ttccagccgc
     4681 tcggccatca gcttgacgac cagcgcggtg ctggacatcg ccatcgcgct gcccaggatc
     4741 gcggcggcct gccacgtgag gtcccagtgc cggccgatca gcgagcagat ccagatgatg
     4801 gcgaagttgc cggccatcgc gcccaggatc gtcagcacca cctgcgaggc gcccagtccg
     4861 aagacgagcg tgcgcatcgc gcgcagcttg ggcaggttga actccagccc gatgacgaac
     4921 atcaggaaga cgacgccgaa ctcggccagg ctgtgcacgc ccgcggtgtc gtgcgccagc
     4981 tgcagcgcgt tgggcccgat gatgacgccg gccaccaggt agccgagcac cggcggcagg
     5041 cgcagcatgc gaaagcccat gacgccggcg atggcggccg ccaggtacag caggacgagt
     5101 tcgagggtcg aggccatgcg ggaccggttt tggcgcgggc aaaaccgcgc ggtgggtggg
     5161 tcgggcagcg ccttgccccg ccacagccgc gggggttccg gacacttaga atccccccat
     5221 cctagaacac aggcagccca ctttgacaaa cgacttgccg ttcgacgccg aacgcgcatt
     5281 gcacctggcc cggcagacct tcgcgatcga ggccgacgcg ctgctcggaa tgcagcgccg
     5341 cctgaccgcc gagttcgcgc gcgcggtcga gacgatcctg cgctgcgagc accgcgtcgt
     5401 cgtgatgggc atgggcaaga gcggccacgt gggccgcaag atcgccgcca cgctggcgtc
     5461 gaccggcacg ccgtcgttct tcgtgcatcc ggccgaggcc gcgcacggcg acctgggcat
     5521 ggtgacggcg ggcgacgtgt gcctgctgat ctccaactcg ggcgagtcca acgagctgag
     5581 cgccatcatc ccagcgctgc gccggctcgg cgtgacgctg gtcgcgctga cgggccgttc
     5641 cgattcgtcg ctggccaagg tgtgcgacat cgtcctgtcc tgcgcggtcg acaaggaagc
     5701 ctgcacgctc aacctcgcgc cgacggccag cacgacggcg cagatggcca tgggcgacgc
     5761 cctggcggtc gctctgctgg actcgcgcgg cttccgcgag gaggacttcg cgctgtcgca
     5821 tccgggcggc gcgctgggcc gcaagctgct gatgcacgtg tccgacctga tgcgctcggg
     5881 cgacgcggtg cccgcggtcg cgcgcgacgc gaacttctcg acgctgctgc aggtcatgtc
     5941 cagcaagggg ctgggcatgg cggcggtggt ggacggccgc acgccggtgg gcatcttcac
     6001 cgacggcgac ctgcgccgcc tcatcgagac cggcgccgac ctgcgccagc gcgtcgcgca
     6061 cgaggtgatg catccgcgcc cgcacctgat ccgcgccggc gcgctggccg tcgacgctgc
     6121 cgaccagatg gaacgccacc gcatcaccag cctgctggtg gtcgacggca agggcgagct
     6181 ggtgggcgcg ctcaacacct atgacctgct gcacgccaag gtgatctgat ggccggcacc
     6241 ccgcagtcgc acctgagctt cccgcccgag atcctggtca aggcgcaagg cgccggcgcc
     6301 ggcatcaagg ccgcgatctt cgacgtcgac ggcgtcctca ccgacggcac gatctggatc
     6361 accgaggccg gcgagacgct caagaacttc aacgtgctcg acggccaggg cctgctgatg
     6421 ctgcgcaagg gcggcatcac gcccatcgtc atcaccgggc gcgattcggc ggcggtgcgc
     6481 cgtcgggtgt ccgacctcgg cctgccgcat gcgtcgttcg gcgcgtccga caaggtggcc
     6541 gcggccaacg acatcctgca gcagctgggc gtggactggg gcgacgtggc cgtcatcggc
     6601 gacgactggc ccgacctgcc gctgttcgcc tgcgccggct tcgcgtgcgc gcccgcgaac
     6661 gcgcacgccg aagcgcgcgg cgctgccgcc tatgtcaccg gacgcgaggg tggccgcggc
     6721 gcggcgcgcg agttctgcga cctgctgctg atggccaacg gcctgtacgc gccgctgatg
     6781 accaagctcc tggtgacgct cgactcgaag tgacctgcgg atgaagggcg aactccacat
     6841 ccccgacctg cccgaggtgc cggtcgcact cagcccgctc agtccgccgg tcgacgacca
     6901 gcagcgccgc acgcggccga gctggcccgt gcgcgtgcgc gagagcctgg tcggctacct
     6961 gccgctgatg ctgatggtgg gtctcgcgct gggcacctgg ctggtggcca agaacacgcc
     7021 ggggctgctg tcggcgtcgg gcccggccgt cgtcaagcac gagcccgact acacgctgga
     7081 ccagttcaac ctgcagcgct tcgacccgtc cggcgtgctc aaggtcgaga tccagggcgt
     7141 gcacatgcag cacttcccgg acgacgacat catggaggtc gagcagatcc gcgtcgtcac
     7201 gaccgagccc gacggccgcc gcatgatcgc caccgcccag cacggccgtg cgcgcggcga
     7261 ctcgaccgag gtgtggctcg acggccaggc gcaggtggtg tccgaggcgc cgggcgagct
     7321 gccgatccag atgaacggcg agcacctgca tgcgctgccc aaggtgcgca aggtcgattc
     7381 gaagtacatg gtggtggtgc gccagggcga cagcgagttc aacgccgacg gcctggagta
     7441 cgacgacctc acgcgcctgc tgacgctgca cggcaacgtg cacgcgctga tgcagccggc
     7501 gctgcgcaag tcgatggcga gcacgagcga cgccaagcga tgacggcgcc gctgctgttc
     7561 atcaccggcg cctccagcgg gatcggccag gcgatggccg tcgagtgggc acggcgcggg
     7621 ggccggctcg ccctggtggc gcgccgcggc gacgagatgc gcgcctgggc agcgtcccag
     7681 ggctggggcg ccgatcgcgc gcaggtctac gtcgccgacg cacgcgacct gcccgccatg
     7741 accgccgccg gtcgcgactg catcgcgcgc cagggcctgc ccgacgtggt gatcgccagc
     7801 gcggggatca gcgtgggcgt cgacatgggc gacgaggccg acctcgaggt gctgcgcgcc
     7861 gtcgtcgaga cgaacaacct cggaatggcc gccaccttcc agccgttcat caagccgatg
     7921 tgcgagcgac gtgccggccg cctggtgggc attgccagcg tggccggcat ccgcgggttg
     7981 cccggtcacg ccgcctattc gatgagcaag gccggcgtga tcagtttctg cgagagcctg
     8041 cgcgtcgaat gccggcgctt cggcgtgcgc gtcgtgacga tcgcgccagg gtatatcgat
     8101 accccgctga cgcgcggcaa ttcatattcg atgccgttcc tgatgccggc cgacaaattc
     8161 gcgcgccagg cgctcgacgc gattgccgca ggcgtcggat tccgggtgat tccctggcag
     8221 atgggggtgt tggcgcgggt gctgcgaata ctgcccgact ggatatacga ccgcgcggtg
     8281 gccggtcgtg gccgcaaggc aagaaagtcg cagtgagcgc tcgctgaggg cgcagagacc
     8341 gaatatctgc gaactgcggg gcgccggtcg cctttgccca cagggtgatc gatcgccgcc
     8401 cgcgggtcgt cacgggggcc ggcgccgact gccagggcgg gccaagaccc gccagacgat
     8461 ggcgccgatg cgacgcttcc tgaaagacgc gatgcctctg gcgccacgag tctgactcgg
     8521 cgctcccagc cgccttgccg gcgcggcaga catttggccg gcgaacggcg gtcgcggccg
     8581 gctcgaccgc gctttccgtc gttgaaattc atcacccggt cgacgcgcca aaatgaaaaa
     8641 gcccggggcc gcgaggcgcc agggcttttg ttcagtcgat ccggctggtt tcagccggac
     8701 agacggatgc gtgtcagagt aacggagcgg gctgctc
//
LOCUS       DATCRO010000555         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_512849, whole genome shotgun sequence.
ACCESSION   DATCRO010000555 DATCRO010000000
VERSION     DATCRO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512849"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VIN75_15150"
     CDS             <1..687
                     /locus_tag="VIN75_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8975554.1"
                     /translation="GDVPPDRFIALAEECGLIGAIDAWVLETACAQLAAWQRDDVPVP
                     SISVNVSPLRFHSDDVPAHVRRLLSLHGLKPGALMLEVTERVMLGDDERTRVDLRTLN
                     EMGVRLSVDDFGTGYSSLGYLKRLPVSELKLDRSFVCDLEREASGRALADAVIGIGQA
                     LGLEVVAEGVETPGQREILARAGCAIAQGFLWTRALPADELVAWLEHERGSWSPRRLT
                     AIPGGGTRAA"
     gene            763..1347
                     /locus_tag="VIN75_15155"
     CDS             763..1347
                     /locus_tag="VIN75_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8975555.1"
                     /translation="MSNDFAAQLETLQPQLLRFARTQLRNEAWAEDAVSETVLAALEK
                     PQSFAGLSQLKTWLVGILKHKVIDQLRRHKREATVLTTDDSEDLDDLLFAPDGHWREM
                     PADWGNPDDVLRQRQFADVLQACLDHLPPTQARVFMMREWLELTSDEVCKELTITPTN
                     LWVLLHRARLRLRDCLQGGWFDAHPAPATAKARR"
     gene            1344..1562
                     /locus_tag="VIN75_15160"
     CDS             1344..1562
                     /locus_tag="VIN75_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8975556.1"
                     /translation="MSLFYRNCREVTRLVLERENRRLTLVERIAVRLHLRICLMCTRF
                     TGQLQLMNRAMAQWKHYAEHDERDDAAT"
     gene            1692..>2596
                     /locus_tag="VIN75_15165"
     CDS             1692..>2596
                     /locus_tag="VIN75_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HEY8975557.1"
                     /translation="MSDLSQPPLNAKPGPHPADRAAAEPSHWAMPPAANAGAAWPLGT
                     SEPCAILGLNDKSFNARLAQFDASIGTAKVFVPPARSPMQLRFGQFQRLTLTEPVMPS
                     TNGVNAVPGWAERAPGDTEFQVQLANGKTHTGRTISHVETDQGLFLFPPVGDAGLVQR
                     HFYPRGAYRRVDFGPRLGDVLVSKHTLSPQQLQDALAEQQRIRNLKLGEILVDVKLIT
                     PAQLQQALELQSRTPMLRLGEALTSLKILSHEDLAQALRSQTRNRNLPLGELLVNHGV
                     ISREELREALEIKRSRLPDDSPSPDT"
BASE COUNT          390 a          971 c          880 g          355 t
ORIGIN      
        1 ggcgacgtcc cgcccgaccg cttcatcgcg ctggccgagg aatgcggcct gatcggcgcg
       61 atcgacgcgt gggtgctgga gacggcctgc gcgcagctgg ccgcctggca gcgcgacgac
      121 gtgccggtgc ccagcatctc ggtcaacgtc tcgccgctgc gcttccattc cgacgacgtg
      181 ccggcgcacg tgcggcgcct gctgtcgctg catggcctga agcccggcgc gctgatgctg
      241 gaggtgaccg agcgcgtgat gctgggcgac gacgagcgca cgcgcgtcga cctgcgcacg
      301 ctcaacgaga tgggcgtgcg gctgtcggtg gacgacttcg gcaccggcta ctccagcctg
      361 ggctacctca agcgcctgcc cgtcagcgag ctcaagctgg atcgcagctt cgtctgcgac
      421 ctcgagcgcg aggccagcgg ccgcgcgctc gccgacgcgg tgatcggcat cggccaggcc
      481 ctgggcctcg aggtggtcgc cgagggcgtg gagacacccg gccagcgcga gatcctggcg
      541 cgcgcgggct gcgcgatcgc ccagggcttc ctgtggacgc gcgccctgcc cgccgacgag
      601 ctcgtggcct ggctggagca cgagcgcggc agctggtcgc cgcgcaggct cacggccatc
      661 cccggcggcg ggacgcgcgc ggcctgagaa gctcaaggcg ggcgtcagcc gggtcggcga
      721 tactcgcgca gcgccgcagt ccggcgcgcc cctgccccgt ccatgtccaa cgatttcgcc
      781 gcccagctcg agacgctcca gccgcaactg ctgcgcttcg cgcgcaccca gctgcgcaac
      841 gaggcctggg ccgaggacgc cgtctccgag accgtgctgg ccgcgctcga gaagccgcag
      901 tcgttcgccg gcctgtcgca gctcaagacc tggctggtgg gtatcctcaa gcacaaggtc
      961 atcgaccagc tgcgccgcca caagcgcgag gccacggtgc tgaccaccga cgacagcgag
     1021 gacctggacg acctgctgtt cgccccggac ggccactggc gcgagatgcc cgccgactgg
     1081 ggcaatccgg acgacgtgct gcgccagcgc cagttcgccg acgtgctgca ggcctgcctg
     1141 gaccacctgc cgcccacgca ggcccgcgtc ttcatgatgc gcgagtggct ggagctgacc
     1201 tccgacgagg tgtgtaagga attgacgatc acgccgacca acctgtgggt gctgctgcat
     1261 cgtgcccggt tgcgcctgcg cgattgcctg cagggcggct ggttcgacgc acatcccgcg
     1321 ccggccacgg cgaaagcaag acgatgagcc tgttctaccg caactgccga gaagtcaccc
     1381 ggctcgtgct cgagcgcgag aaccgcaggc tcacgctcgt cgagcgcatc gccgtgcgct
     1441 tgcatctgcg catctgcctg atgtgcacgc gcttcaccgg gcagctgcag ctgatgaacc
     1501 gcgcgatggc gcagtggaag cactacgccg agcacgacga gcgcgacgac gcggccacct
     1561 gaacgcgcgt cccggcgaac tgccccgcga tcggcccgct atttgcggtt tcgcggcagg
     1621 gctcgccacc taggatcgac tggcccccag ccttcgccgg ggaaaaggcg cgttcgcgcg
     1681 ctacaaaagc catgtccgac ctttcgcagc ctcccctgaa cgccaagccg ggcccgcacc
     1741 cggccgaccg tgccgccgcc gagccgtcgc actgggccat gccgcccgcc gcgaacgcgg
     1801 gcgccgcctg gccgctgggc acgagcgagc cgtgcgcgat cctgggcctg aacgacaaga
     1861 gcttcaacgc ccgcctggcg cagttcgacg cctcgatcgg cacggccaag gtcttcgtgc
     1921 cgcccgcgcg ttcgccgatg cagctgcgct tcggccagtt ccagcgcctg acgctgaccg
     1981 agccggtgat gccgtcgacc aacggcgtca acgcggtgcc gggctgggcc gagcgcgcgc
     2041 cgggcgacac cgagttccag gtgcagctgg ccaacggcaa gacgcacacg gggcgcacga
     2101 tcagccacgt cgagaccgac cagggcctgt tcctgttccc gcccgtgggc gatgccggcc
     2161 tggtgcagcg ccacttctac ccgcgcggcg cctaccgtcg cgtcgacttc gggccgcgcc
     2221 tgggcgacgt tctcgtcagc aagcacacgc tgtccccgca gcagctgcag gacgcgctcg
     2281 ccgagcagca gcgcatccgc aacctcaagc tcggcgagat cctcgtcgac gtcaagctca
     2341 tcacgccggc gcagctgcag caggcgctcg aactgcagtc gcgcacgccg atgctgcggc
     2401 tgggcgaggc gctcacctcg ctgaagatcc tctcgcacga ggacctcgcg caggccctgc
     2461 gcagccagac gcgcaaccgc aacctgccgc tgggcgaact gctcgtcaac cacggcgtga
     2521 tctcgcgcga ggagctgcgc gaggccctgg agatcaagcg ctcgcgcctg cccgacgact
     2581 cgccctcgcc cgacac
//
LOCUS       DATCRO010000556         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1120158, whole genome shotgun sequence.
ACCESSION   DATCRO010000556 DATCRO010000000
VERSION     DATCRO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120158"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="gatB"
                     /locus_tag="VIN75_15170"
     CDS             complement(<1..747)
                     /gene="gatB"
                     /locus_tag="VIN75_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8975558.1"
                     /translation="MSTLIRGYEVVIGFETHTQLSTASKIFSGASTEFGAEPNTQASA
                     VDLALPGTLPVLNRGAVERAIRLGLAVNATVAPLSIFARKNYFYPDLPKGYQISQYEI
                     PVVQGGTVPFVHNGELRTLRLTRAHLEEDAGKSVHEGFDNMSGIDLNRAGTPLLEIVT
                     EPDLRSSTEAVDYARSLHALVMWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIK
                     NLNSFRHLQQAIDYEINWQIDELEDGRAIVQ"
     gene            complement(744..1697)
                     /locus_tag="VIN75_15175"
     CDS             complement(744..1697)
                     /locus_tag="VIN75_15175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY8975559.1"
                     /translation="MNARADHLPPATPVRGGVVAGDCLDVLRSMPGERVDLIVTSPPY
                     ADQRASTYGGVTADDYVDWFMPRAEQFARVLKREGSLVINIKEKVVDGERHTYVLELI
                     LAMRKQGWLWTEEYVWHKRNCFPGKWPNRFRDAWERCLHFTRQKRFKMNQDAVMVPMG
                     DWAQTRLKTLGANDVVRHDSRVGNAFGKNIANWQGRDRAYPTNVLHLATECGNKGHSA
                     AFPQALPRWFIELFTDPGDTVLDPFAGSGTTLAAALALGRNAIGVELAPAYVTRARDA
                     VASASEAAQALARASLVTGQDIISPDHGDAVATVSKDSVPS"
     gene            complement(1706..3199)
                     /gene="gatA"
                     /locus_tag="VIN75_15180"
     CDS             complement(1706..3199)
                     /gene="gatA"
                     /locus_tag="VIN75_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8975560.1"
                     /translation="MSGELHTMGVAGLGRALAARSVSSVEATQAFLARAKADQHGAFL
                     VVDEDAAIAAATAADARRAAGAEGSSNPLLGVPIAHKDIFVTTESPTTAGSKMLQGYR
                     SPFDATVVARLKAAGTVSLGKLNCDEFAMGSANENSAYGVVTNPWDATRVPGGSSGGT
                     AAAVAARLVPAATGTDTGGSIRQPASFTGTTGIKPTYGVCSRFGMIAFASSLDQAGTI
                     AHSAEDCALMLSAMSGFDERDATSVQRPPQDFHAQMLQARPGASPARPLEGLRIGLPR
                     EFFPAALAADVGGRVREALAQMEKLGATLVDVSLPRTELSIPVYYIIAPAEASSNLSR
                     FDGVRYGHRAAKYDDLLDMYKKSRSEGFGPEVKRRIMIGTYVLSHGYYDAYYLQAQKI
                     RRMIADDFQACFQQCDLIAGPVAPTVAWKLGEQGDDPVKAYLADIFTLPGSLAGLPGM
                     SVPAGLGDGGMPVGLQLLGNYFAEGMLLHAAHALQQATDFHQQRPKA"
     gene            complement(3196..3495)
                     /gene="gatC"
                     /locus_tag="VIN75_15185"
     CDS             complement(3196..3495)
                     /gene="gatC"
                     /locus_tag="VIN75_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8975561.1"
                     /translation="MPLTPEDVSRIAHLARLELSPAEGPAMQTQLNDFFKIVEQMSAV
                     DTSGLEPLHTPLSAIGNVELRLRPDAVTETNRREANMANAPAAEEGLFLVPKVIE"
     gene            3868..>4058
                     /locus_tag="VIN75_15190"
     CDS             3868..>4058
                     /locus_tag="VIN75_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8975562.1"
                     /translation="MFSSLRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVSIRHE
                     GGPHGKKIIQAVGKEAKAML"
BASE COUNT          595 a         1373 c         1451 g          639 t
ORIGIN      
        1 ctgcacgatg gcgcggccgt cctccagctc gtcgatctgc cagttgatct cgtagtcgat
       61 cgcctgctgc aggtggcgga agctgttgag gttcttgatc tcgcggcgcg tgccgaacgg
      121 cgcgccgggg cggcgcacgg acacgttggc gtcgcagcgg aacgagcctt cctgcatgtt
      181 gccgtcgcag atgcccagcc acatcacgag cgcgtgcagc gacctggcgt agtcgacggc
      241 ctcggtggac gagcgcaggt ccggctcggt cacgatctcc agcagcggcg tgccggcgcg
      301 gttcaggtcg atgcccgaca tgttgtcgaa cccctcgtgc accgacttgc cggcgtcctc
      361 ctcgaggtgc gcgcgcgtca ggcgcagcgt gcgcagctcg ccgttgtgga cgaacggcac
      421 tgtgccgccc tgcacgacgg ggatctcgta ctggctgatc tggtagccct tgggcaggtc
      481 gggatagaaa tagttcttgc gcgcgaagat cgacagcggc gccacggtgg cgttgaccgc
      541 caggcccagt cggatcgcgc gctcgaccgc gccgcggttc aggacgggca gcgtgccggg
      601 cagcgcgagg tcgaccgcgg aggcctgcgt gttgggctcg gcgccgaact cggtggacgc
      661 accggagaag atcttgctgg ccgtcgacag ttgcgtgtgg gtctcgaagc cgatcacgac
      721 ttcatagccg cgaatgaggg tgctcatgat ggaacgctgt ccttggaaac ggtggccacg
      781 gcatcgccgt ggtccgggga aatgatgtct tgacctgtga cgaggctggc gcgcgcgagc
      841 gcctgcgcgg cctcgctcgc gctcgcgacc gcgtcacgcg cgcgcgtgac gtacgcgggc
      901 gcgagctcga ccccgatcgc gttgcgaccg agcgccagcg ccgcggccag cgtggtgccg
      961 gagccggcga acgggtccag cacggtgtcg cccgggtcgg tgaacagctc gatgaaccag
     1021 cgcggcagcg cctgcgggaa cgccgcgctg tggcccttgt tgccgcactc ggtggccagg
     1081 tgcagcacgt tggtcgggta cgcgcggtcg cggccctgcc agttggcgat gttcttgccg
     1141 aaggcgttgc ccacgcgcga gtcgtggcgc accacgtcgt tggcgccgag cgtcttcagg
     1201 cgcgtctgcg cccagtcgcc catgggcacc atgacggcgt cctggttcat cttgaagcgc
     1261 ttctggcgcg tgaagtgcag gcagcgctcc cacgcgtcgc ggaagcggtt gggccacttg
     1321 ccgggaaagc aattgcgctt gtgccagacg tactcctccg tccacagcca gccctgcttc
     1381 ctcatcgcga ggatcagctc gaggacgtag gtgtggcgct cgccgtcgac caccttctcc
     1441 ttgatgttga tcaccaggga gccttcgcgc ttgagcacgc gcgcgaactg ctcggcgcgc
     1501 ggcatgaacc agtcgacgta gtcgtcggcg gtgacgccgc cataggtcga cgcgcgctgg
     1561 tcggcatagg ggggagacgt cacgatcagg tcgacgcgtt cgcccggcat cgagcgcagc
     1621 acgtcgaggc aatcacccgc gaccacgccg ccacggaccg gcgtggcggg aggcagatgg
     1681 tcggcgcgcg cgttcatcgg caggctcagg ccttcgggcg ctgctggtgg aagtcggtcg
     1741 cctgctgcag cgcgtgggcc gcgtgcagca gcatgccttc cgcgaagtag ttgcccagca
     1801 gctgcaggcc caccggcatg ccgccgtcgc ccaggcccgc cggcacgctc atgccgggca
     1861 ggccggcgag cgagccgggc agcgtgaaga tgtcggcgag gtaggccttg accgggtcgt
     1921 cgccctgctc gccgagcttc catgccaccg tcggcgccac cgggccggcg atcaggtcgc
     1981 actgctggaa gcaggcctgg aagtcgtcgg cgatcatgcg gcggatcttc tgggcctgca
     2041 ggtagtacgc gtcgtagtag ccgtgcgaga gcacgtacgt gccgatcatg atgcggcgct
     2101 tgacctcggg gccgaaacct tcggagcgcg acttcttgta catgtccagc aggtcgtcgt
     2161 acttcgcggc gcgatgcccg tagcgcacgc cgtcgaagcg gctcaggttc gagctcgcct
     2221 cggccggcgc gatgatgtag tacacgggga tcgacagctc ggtgcgcggc agcgagacgt
     2281 ccaccagcgt ggcgcccagc ttctccatct gcgccagcgc ctcgcgcacg cggccgccga
     2341 cgtcggcggc cagcgcagcg gggaagaact ccctgggcag gccgatgcgc agcccctcga
     2401 gcggccgcgc tggcgacgcg ccggggcgcg cctgcagcat ctgcgcgtgg aaatcctgcg
     2461 gcgggcgctg cacgctggtg gcgtcgcgct cgtcgaagcc gctcatcgcc gacagcatca
     2521 gcgcgcagtc ctcggccgag tgcgcgatgg tgccggcctg gtcgaggctg gacgcgaacg
     2581 cgatcatgcc gaagcgcgag cacacgccgt acgtcggctt gatgccggtg gtgccggtga
     2641 agctcgcggg ctggcggatc gagccgccgg tgtcggtgcc ggtggccgcg ggcacgaggc
     2701 gcgcggcgac cgcggcggcg gtgccgcccg acgagccgcc cggcacgcgc gtggcgtccc
     2761 agggattggt cacgacgccg tacgcggagt tctcgttggc cgagcccatc gcgaactcgt
     2821 cgcagttgag cttgcccagc gacacggtgc ccgccgcctt caggcgcgcg acgacggtcg
     2881 cgtcgaacgg gctgcggtag ccctgcagca tcttcgagcc ggccgtggtg ggcgactcgg
     2941 tcgtgacgaa gatgtccttg tgcgcgatgg gcacgcccag caacgggttc gacgaccctt
     3001 cggcgcccgc ggcgcgcctg gcgtcggcgg ccgtggcggc ggcgatcgcg gcgtcctcgt
     3061 cgacgaccag gaaagcgccg tgctggtcgg ccttggcgcg cgccaggaag gcctgcgtgg
     3121 cctcgacgct ggagacggac cgggcggcca gcgcgcggcc gagcccggcc acgcccatcg
     3181 tgtgcagttc gccgctcatt cgatcacctt gggcaccagg aacaggcctt cctcggccgc
     3241 gggcgcgttg gccatgttgg cctcgcgacg gttggtctcg gtgacggcgt cgggccgcag
     3301 gcgcagctcg acattgccga tggccgacag cggcgtgtgc agcggctcga ggccggaggt
     3361 gtcgaccgcg ctcatctgct cgacgatctt gaagaagtcg ttcaactggg tctgcatcgc
     3421 aggcccctcg gcgggcgaca gctcgagccg cgcgagatgc gcgatgcggc tgacgtcttc
     3481 gggagtcaag ggcatgggga aatctcagct ggggtcggcg gcccgcgata cccggaccag
     3541 gggagccggg aagggtgccg agctaaaaaa gttacgacct gagtacaggc tcatcaggtg
     3601 cgcttgcggt attatcctcg gttaattcga ggcatcgtgc acgagctgat tcatgacctg
     3661 aatccagcgt cgccgggcgc ccggacccac ccaattcatg cgccttgcgg cgccacgagc
     3721 gagacgcaca tccaggttcg gaatccccgg cccgggcctc ccggccccgc cccccggttc
     3781 cggagtcata cccatccaga tggcctgata ccgaccccgc gacgacccgc cgcaagccgc
     3841 cgacccaaac gccaccccct cccgccaatg ttctcttcgc tgcgccgcta tttctccacc
     3901 gatctcgcga tcgacctcgg gaccgccaac acgctgatct acgtgcgcgg caagggcatc
     3961 gtcctggacg aaccctcggt ggtctcgatc cgccacgaag gcggcccgca cggcaagaag
     4021 atcatccagg ccgtcggcaa ggaagccaag gcgatgct
//
LOCUS       DATCRO010000557        15266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_444794, whole genome shotgun sequence.
ACCESSION   DATCRO010000557 DATCRO010000000
VERSION     DATCRO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444794"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="ftsA"
                     /locus_tag="VIN75_15195"
     CDS             complement(<1..533)
                     /gene="ftsA"
                     /locus_tag="VIN75_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8975563.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEILPGGELRLAGLGVAPTHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKISRVYTGITGSHIRGQNSTGMVIVRDNR
                     EVTPTDMARVVETAKAINIPNDQRVLLVEPQEFVIDGHEVKEPIGMSGSRLEVRVHIV
                     TGAQSAAENIVKCVRRCG"
     gene            complement(554..1369)
                     /locus_tag="VIN75_15200"
     CDS             complement(554..1369)
                     /locus_tag="VIN75_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY8975564.1"
                     /translation="MLLRQPATPDAAPAQALPVDVRLMNAIAAIVFVLALVALAAAAI
                     AWAARRPQFQFADVRLEGDLQRNSVTTVRANALPHLAGNFFTMDLARTRAAFEQVPWV
                     RHAVVRRVWPDRLVVALEEHQPVALWEGDENSDKMVNSHGEVFEANVGDVEDDSLPQF
                     AGPEGTSAQVLDMYRRLQPVFSKMDAEMNALRLSGRGSWKVDLDDGATIELGRGTQDE
                     VVDRTARFVRTVPQLLDKFHAPLESADLRHAEGYAVKLKGLTVNADAKQPAGK"
     gene            complement(1377..2393)
                     /locus_tag="VIN75_15205"
     CDS             complement(1377..2393)
                     /locus_tag="VIN75_15205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY8975565.1"
                     /translation="MNFISLSRDQAARLGKIAVLMGGTSAEREISLLSGNGVLAALKS
                     GGFDAHAFDPAQRPMDDLKREGFQRCFIALHGRHGEDGAIQGALELMGIPYTGPGMMA
                     SAIAMDKVMTKRVWRAEVLSTPRGRVLSPEEQVDRFISKVPDELGLPLIVKPPHEGSS
                     VGVTKVRSWAQMKEAVELAAGYDADVLCEEFIDGDEVTVAVLGSGMDTMALPVVRIVA
                     PEGKYDLQNKYFTDVVKYHCPSGLPDAEEKAVQRLAVAAYRALDCRGWSRADLIIRAS
                     DRKPFLIEMNTSPGMTSHSLVPMAARAVGVSYEALCAHLIASAALDPKGKGGKPAKNE
                     QPGT"
     gene            complement(2390..3823)
                     /gene="murC"
                     /locus_tag="VIN75_15210"
     CDS             complement(2390..3823)
                     /gene="murC"
                     /locus_tag="VIN75_15210"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8975566.1"
                     /translation="MKHAVKKIHFVGVGGAGMSGIAEILHNLGYAVSGSDQADSATSR
                     RLATLGIRVFTGHDAANVADAQAVVTSTAVKGDNPEVIAARARRIPVVPRAVMLAELM
                     RLKKGIAIAGTHGKTTTTSLVTSVLAEAGVDPTFVIGGKLNSAGANSRLGSGEYIVVE
                     ADESDASFLNLMPILSVVTNIDADHMDTYGHDFARLKAAFVEFLHRMPFYGSAIVCGD
                     DPGVRSIVPMISRPVITYGMNEDVQVRAVDVRALEGGQMQFTCRRRNGVQMADLAVTL
                     NLPGEHNVRNALAAIAVATELELPDDAIVRALAGFTGVGRRFQSYGQQAVDADKGGGS
                     FTLIDDYGHHPVEMAAVIAAARGAFPRHRLVLAFQPHRFTRTRDCFEDFVKVMGQADQ
                     VLLTEVYSAGEAPIVAADGRSLARALRVAGKVDPVFVDDVGELAAAIIDHARDGDVVI
                     SMGAGSIAAVPQKVADLRAQARNGGTQ"
     gene            complement(3861..4958)
                     /gene="murG"
                     /locus_tag="VIN75_15215"
     CDS             complement(3861..4958)
                     /gene="murG"
                     /locus_tag="VIN75_15215"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8975567.1"
                     /translation="MSSDLATRAPRHLVVMAAGTGGHVMPGLAVAREVLARGWTVSWL
                     GTRTGMENRLVPPSGIPMDTIDFAGVRGKGLLGTFKGLMQLAAAFGACRHILRTRNAS
                     AVLGMGGYVCVPGGKVARRLRKPLVLVNADAALLLSNKSLLSSCDRIAFGFDGDDARA
                     TPKALVTGNPVRAEIEALSPPAERLAQRAGPLRVLVVGGSLGAKVLNETLPQALKLIP
                     AGERPRVTHQTGEKNWELVQAEYLARGVEAEVVPFIDDMPRRLAECDLIVCRAGAITV
                     SELCAAGVASILVPLVISTTSHQRDNAQYMASRGAARHLPQAELTPERLAAELRGATR
                     ANLLAMAERARSLARPQAAARVADELERLVA"
     gene            complement(4955..6235)
                     /gene="ftsW"
                     /locus_tag="VIN75_15220"
     CDS             complement(4955..6235)
                     /gene="ftsW"
                     /locus_tag="VIN75_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY8975568.1"
                     /translation="MSVMALLKQHLPEWPGFRPAEWFRKRRAAAAGDERVPIRDWVDA
                     GRPTRVMGVDSALIWMTAALLAFGLVMVYSASVALPDSPRFALYSSTHFLGRQAMFIA
                     IGSVCAFATAQVPIAIWEKWAPWIFALAMVLLALVLIPHVGKGVNGARRWIPLGVLNF
                     QPSELAKLGMCMYAASYMVRKMDVKENFIKAVWPMAVALGVIGVLLLAEPDMGAFMVI
                     AAIALGVLFLGGVNGRMFLLSVAVLLGAFVLMIVFSEFRRQRIFAYLDPWNPTYAQGK
                     AYQLTHSLIAFGRGEIFGQGLGNSIEKLHYLPEAHTDFLLAVVGEELGFVGVFIIVAA
                     FFWLTRRLFIIGRQAIALDRVFAGLMCQGVGLWMGAQSFINFGVNLGVLPTKGLTLPL
                     LSYGGSGVVLNLVALALVLRVDMESRALMRGGRA"
     gene            complement(6232..8385)
                     /gene="murD"
                     /locus_tag="VIN75_15225"
     CDS             complement(6232..8385)
                     /gene="murD"
                     /locus_tag="VIN75_15225"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8975569.1"
                     /translation="MSEQSPNTVLILGLGASGLAMARWCAGQGARVRVWDSRPNPPQA
                     AALAAEVEGSTFFSGELTAAALDGVARVYKSPGLSPADPRLLTLLQAARDEGVPVRGE
                     LDLFSVALGQLKAERGYAPKILAITGTNGKTTTTSLTGQLVERAGRSVAVAGNIGPTM
                     LDTLAAAIAAQAVADVEAAEAAAAQALLDAEEAAARAAQDAIDAEAARVAAEKQARLD
                     AELAAAAAARQAEEDAKAQARAEAAAAKAARAQARAAKAAAKANPDAELEMPVEAEVP
                     TDVAAAEAVPVDADTPLADAQSRELVETEDADELRELAALDGSDVPAQLAEDVDEAHA
                     DEQLAEDSAELAEAAATLPDDDAPAITLIPPPPADPVFSHLPQVWVLELSSFQLDEVH
                     DFNPSAATVLNITQDHLDWHGSMKAYAAAKARIFGTDAMPATVMVINRDDPAVEAMVP
                     PPVIVKPTRRGERAKQVHRRVVRFGLDAPREPGDFGLVEEAGMAWLVRAMELDPTIKR
                     KPGDEIDLVIQRLMPADALRIRGRHNASNALAALALASSVGIPLAPMLHGLREYRGEP
                     HRVEPVGIIGQVEFFDDSKGTNVGATVAALNGLGQDRAPSKLVVILGGDGKGQDFVPL
                     TEPLRRHARAAATIGRDAEALEAVLRTASVPFERFATLEEATRWAHQQAHGSDAVLLS
                     PACASLDMFRDYKHRADVFVATVHDIADDAGEVLA"
     gene            complement(8390..9571)
                     /gene="mraY"
                     /locus_tag="VIN75_15230"
     CDS             complement(8390..9571)
                     /gene="mraY"
                     /locus_tag="VIN75_15230"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8975570.1"
                     /translation="MLLSLTSWLQEHYPQSFGFLRVFQYHTFRAVMAAMTALLIGLFF
                     GPWVIRRLTELKIGQPIREYGVQSHMVKRGTPTMGGVLVLIAIAIATLLWSDWSNRFV
                     WIVMLVTFGFGAIGWSDDWRKVVQKNPEGMPSREKYMWQSLIGLVAALYLAFSVSETS
                     NLRVLELFLRWVTSGFANDLPPKADLMLPFFKTVSYPLGVFGFIAMTYFVIVGASNAV
                     NLTDGLDGLAIMPVVLVAGALGLFAYVVGNAVYSRYLLFPYIPGAGELLIFCAAIAGA
                     GLAFLWFNAHPAQVFMGDVGALALGGALGTIAVITRQEIVLGIMGGVFVAEALSVMAQ
                     VSYFKYTKKKYGEGRRILKMAPLHHHFEKSGWTETQVVIRFWIVTMLLCLVGLASLKL
                     R"
     gene            complement(9561..10973)
                     /gene="murF"
                     /locus_tag="VIN75_15235"
     CDS             complement(9561..10973)
                     /gene="murF"
                     /locus_tag="VIN75_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8975571.1"
                     /translation="MMTLAEAVAMVPGAVLVGDGARAFERVHTDTRTLRAGDLFVALQ
                     GERFDAHDFLPQARAAGAVAAIGTHGVAEAGLDGLVVTDSLQALQALATGWRRRFAGP
                     VVAVTGSNGKTTVTQMIASILRAAHADAAHATAGNLNNHIGVPLTVLRMDASHKSAVI
                     ELGMNHPGEIAVLARIAQPTVALVNNAQREHQEFMGTVEAVARENGAALEALPADGVA
                     VFPCDDEYADIWQAQAAGRRCLRFALAGEAEGPWPRAEISARARWVGDAWEVEMTTPE
                     GQARLALRVAGRHNVRNALASTAAALGAGIALADVVRGLESFAPVKGRSQLKSLQRGG
                     RTIPLVDDTYNANPDSVRAAIDVLAGLPAPRWLLLGDMGEVGDQGPAFHQEIGAYAKA
                     RGIDEFWCAGGLCAHAARAYGPDARHFDDTPALLAALGDARDSEPAFASVLAKGSRFM
                     GMERVVALLQSHDDGGAHAA"
     gene            complement(10979..12517)
                     /locus_tag="VIN75_15240"
     CDS             complement(10979..12517)
                     /locus_tag="VIN75_15240"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8975572.1"
                     /translation="MALTRLKSPAAAARWLKSWVTGTLRTDSRAVQPGDAFIAWPGYA
                     NDGRRFVASALAAGASTCLVEQDQVDAFGFEDARIAALTSMKAATGHIAAAFFDEPSE
                     RLKVVATTGTNGKTSTAWWTSQALNLLGRRTGVVGTLGIGVPPLPMGVAAADSRQDWT
                     IRATGLTTPDPVALQAAFRRMVDDGFAACAIEASSIGIVEQRLAGTRIEVALFTNFTR
                     DHLDYHGSMDSYWAAKRLLFGWPGLKAAVVNIDDDAGDLLAGELTKGPLDLWTYSTRG
                     DSPARLVAEDIGYRDGGLAFTLVESGTERAAVATTLIGDYNVHNLLAVIGGLRALGVT
                     LADAARVAGLVTPVPGRMQRVAANAAGQPEVVVDYAHTPDALEKVIAALRPLAADRGG
                     RLHCVFGCGGDRDATKRPLMGGIAARLADAVVVTSDNPRTEDPRKIVDQVAAGATGGA
                     PVQVIVDRRAAIAQVLAGADARDVVLVAGKGHEDYQEINGVRQHFSDVEEALAGLRKR
                     GLAA"
     gene            complement(12599..14377)
                     /locus_tag="VIN75_15245"
     CDS             complement(12599..14377)
                     /locus_tag="VIN75_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8975573.1"
                     /translation="MSKANKRVQTPRGRAVSNVTYANSPLLASKTPLWRSRFLVVLVG
                     IGSLILVGRAAYIQIIATNFYLKQGEARYAHTLEMPASRGRIVDRNGLLLATSVPTPS
                     IWAIPKDVDADAGEKKQLAKALGMTVAELDDRLDGNPNFVWLKRQVDDSVGAAVKALD
                     LKGIHQVSEFRRRYPEGEAAAHVVGFTGDQDKGQEGIELAYQQQLQGRDGQREVVKDR
                     LGRIVEDIGDPQRPINGEDIELSIDSKVQFFAYQRIRDQVIAQKAKGGSVVVIDTLTG
                     EVLALANYPSYNPGDRHHLSGAQLRNRALTDVFEPGSTMKPFIVGTALEKGLVTPTSM
                     VDATPGSLVINGFAPKDAEPNGWLTVQQVIQKSSNIGAARLAMKLTPKEEWEKFSSIG
                     LGQKPQINFPGVVTGRLRPYKTWRPIEQATMAYGYGLSASLFQLARAYTVFANDGVLE
                     NVSMLRQSAPGPGPRVFKPETVKELNTMLQMVASPGGTAPKARTLGYSVGGKSGTAYK
                     AEGRGYAQNKYRPWFVGLAPISHPRIVVAVMVDEPTAGLHYGGDVAAPVFSQVVSQTL
                     PLLGVAPDLDVRTQITQANVVQIPEN"
     gene            complement(14374..14736)
                     /gene="ftsL"
                     /locus_tag="VIN75_15250"
     CDS             complement(14374..14736)
                     /gene="ftsL"
                     /locus_tag="VIN75_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY8975574.1"
                     /translation="MSGQSFMVRLNVLLAIALIVSGLYLVKISYESRRLFAEIENAHG
                     TERQLESDRRRLEAERQAQATHLRVEATARTKLNMRAATPANTQYVVDAGLAAAPQSA
                     PLARTAAPASVPDSGAAQ"
     gene            complement(14733..>15266)
                     /gene="rsmH"
                     /locus_tag="VIN75_15255"
     CDS             complement(14733..>15266)
                     /gene="rsmH"
                     /locus_tag="VIN75_15255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8975575.1"
                     /translation="RGETAADFLARADERHIAEVIRDYGEERFAVSIAKAIVALRDGG
                     TPVRTTRELSAVVARSVKTREAGQDPATRTFQALRIFVNAELEELEQVLNASLDLLEE
                     GGRLAVISFHSLEDRIVKTFIARESKAEIDRRAPFAPPAKALRLNALGRIKPGPTEVL
                     NNGRARSAILRVAERLA"
BASE COUNT         2137 a         5455 c         5390 g         2284 t
ORIGIN      
        1 ccgcagcggc gcacgcactt gacgatgttc tcggccgcgc tctgcgcgcc ggtgacgatg
       61 tgcacgcgca cctcgagccg gctgccgctc atgccgatcg gctccttgac ctcgtggccg
      121 tcgatcacga attcctgcgg ctcgaccagc agcacgcgct ggtcgttggg gatgttgatc
      181 gccttggccg tctccaccac gcgcgccatg tcggtgggcg tgacctcgcg gttgtcgcgc
      241 acgatcacca tgcccgtcga attctggccg cggatgtggc tgccggtgat gcccgtgtag
      301 acgcgcgaga tcttgcagtc ggccatcatc tcggcctcct tcaaggcctg ctggatggac
      361 tgcaccgtgg cgtcgatgtt cacgacgacc ccgcgcttca agccatgcgt cggcgcgacg
      421 cccaggcccg cgagcctcag ctcgccgccc ggcagtatct cggcgacgac ggccatcacc
      481 ttggcggtgc cgatgtccag gccgacgacg agatccttgt attccttggc cattgctgtg
      541 cgtttccctc ttgctacttg cccgccggct gcttcgcgtc ggcgttcacc gtgaggccct
      601 tgagcttcac ggcgtagcct tcggcatgcc gcagatccgc gctctcgagc ggcgcgtgga
      661 acttgtcgag cagctgcggc acggtgcgca cgaagcgcgc cgtgcggtcg accacctcgt
      721 cctgcgtgcc gcgacccagt tcgatcgtgg cgccgtcgtc caggtcgacc ttccagctgc
      781 cgcggcccga cagccgcagc gcgttcatct cggcgtccat cttcgagaac acgggctgca
      841 ggcggcggta catgtccagc acctgcgccg aagtgccctc ggggcccgcg aactgcggca
      901 ggctgtcgtc ctcgacgtcg ccgacgttgg cctcgaagac ctcgccgtgg ctgttgacca
      961 tcttgtcgga gttctcgtcg ccttcccaca gcgcgacggg ctggtgctcc tcgagcgcga
     1021 cgaccagcct gtcgggccac acgcggcgca cgacggcatg gcgcacccag ggcacctgct
     1081 cgaacgcggc gcgcgtgcgg gcgaggtcca tcgtgaagaa attgccggcc aggtgcggca
     1141 aggcattcgc gcgcacggtg gtcacgctgt tgcgctgcag gtcgccctcc agccgcacgt
     1201 cggcgaactg gaactgcgga cgccgggccg cccaggcgat ggccgcggcc gccagcgcca
     1261 ccagcgccag cacgaacacg atcgcggcga tcgcattcat gaggcgcacg tccaccggca
     1321 gcgcctgggc gggcgcggca tcgggggtgg cgggttggcg caggagcatt tgcggctcag
     1381 gttccgggtt gttcgttctt tgcgggcttg ccgcccttgc ccttggggtc gagcgcggcc
     1441 gaggcgatca gatgcgcgca cagcgcctcg taggacacgc ccaccgcgcg cgcggccatc
     1501 ggcaccagcg agtgcgaggt catgcccggc gaggtgttca tctcgatcag gaacggcttg
     1561 cggtcggacg cgcggatgat caggtcggcg cggctccagc cgcggcagtc gagcgcgcgg
     1621 taggccgcga cggccaggcg ctgcacggcc ttctcctcgg cgtcgggcag gccgctcggg
     1681 cagtggtact tcacgacgtc ggtgaagtac ttgttctgca ggtcgtactt gccttcgggc
     1741 gcgacgatgc gcaccacggg cagcgccatc gtgtccatgc ccgagcccag caccgccacg
     1801 gtgacctcgt cgccgtcgat gaactcctcg cacagcacgt cggcgtcata gccggcggcc
     1861 agttcgaccg cctccttcat ctgcgcccag ctcctgacct tggtcacgcc caccgacgac
     1921 ccctcgtgcg gcggcttgac gatcagcggc aggcccagtt cgtcgggcac cttggagatg
     1981 aacctgtcga cctgctcctc ggggctcagc acgcgcccgc gcggcgtgga cagcacctcg
     2041 gcgcgccaca cgcgcttggt catcaccttg tccatcgcga tggccgaggc catcatgccc
     2101 gggcccgtgt acgggatgcc catcagctcg agcgcgccct ggatcgcgcc gtcctcgcca
     2161 tggcggccgt gcagcgcgat gaagcagcgc tggaagcctt cgcgcttcag gtcgtccatc
     2221 ggacgctggg cgggatcgaa cgcatgcgcg tcgaagccgc ccgacttcag cgccgccagc
     2281 acgccgttgc ccgagagcag cgagatctcg cgttcggcgg acgtgccgcc catcagcacg
     2341 gcgatcttgc cgagcctggc ggcctggtcg cggctcaatg aaatgaagtt cattgcgtgc
     2401 ctccgttgcg tgcctgcgcc cggaggtccg ccaccttctg cggcaccgcg gcgatcgagc
     2461 cggcgcccat cgagatgacg acgtccccgt cgcgcgcgtg atcgatgatg gccgcggcga
     2521 gctcgccgac gtcgtcgacg aacaccggat ccaccttgcc cgccacgcgc agcgcgcgcg
     2581 ccagcgagcg gccgtcggcg gccacgatgg gcgcctcgcc ggccgagtac acctcggtca
     2641 gcagcacctg gtcggcctgg cccatcacct tgacgaagtc ctcgaagcag tcgcgggtgc
     2701 gcgtgaagcg gtgcggctgg aaggcgagca ccagccggtg gcgcgggaac gcgccgcgcg
     2761 cggcggcgat gacggcggcc atctccaccg ggtggtggcc gtagtcgtcg atcagcgtga
     2821 agctgccgcc gcccttgtcg gcgtcgacgg cctgctggcc gtagctctgg aagcggcggc
     2881 ccacgccggt gaagccggcc agcgcgcgga cgatcgcgtc gtcgggcagc tccagctcgg
     2941 tggccaccgc gatcgcggcc agcgcgttgc gcacgttgtg ctcgccgggc aggttcagcg
     3001 tcaccgcgag gtcggccatc tgcacgccgt tgcggcgccg gcaggtgaac tgcatctggc
     3061 cgccctcgag cgcccgcacg tccaccgcgc gcacctgcac gtcctcgttc atgccgtagg
     3121 tgatgacggg gcgcgagatc atcggcacga tcgagcgcac gccgggatcg tcgccgcaca
     3181 cgatggccga gccgtagaac ggcatgcggt gcaggaactc cacgaacgcg gccttcaggc
     3241 gcgcgaagtc gtggccgtag gtgtccatgt ggtcggcgtc gatgttcgtg accaccgaca
     3301 ggatcggcat caggttcagg aacgaagcgt ccgactcgtc ggcctcgacc acgatgtact
     3361 cgcccgagcc caggcgcgag ttcgcgccgg cggagttgag cttgccgccg atcacgaagg
     3421 tcgggtcgac gcccgcctcg gccagcacgc tggtgaccag cgacgtggtc gtcgtcttgc
     3481 cgtgcgtgcc ggcgatcgcg atgcccttct tcaggcgcat cagctcggcc agcatcaccg
     3541 cccgcggcac caccgggatg cgcctggcgc gcgccgcgat cacctcgggg ttgtcgccct
     3601 tgaccgccgt cgacgtgacc acggcctgcg cgtcggccac gttggccgcg tcatggcccg
     3661 tgaagacgcg gatgcccagc gtcgcaaggc ggcgcgaggt cgcgctgtcg gcctggtcgg
     3721 agccggagac ggcgtagccg aggttgtgca ggatctcggc gatgccggac atgccggcgc
     3781 cgccgacgcc aacgaaatgg atcttcttga cggcgtgctt catgcgcacc tccgcttcat
     3841 gttcaacagc ggactcgggt tcatgccacg agcctttcga gttcatccgc gacgcgcgcg
     3901 gcggcctggg gacgcgcgag cgaacgcgcg cgttcggcca tggcgagcag gttggcgcgg
     3961 gtcgccccgc gcagttcggc ggccagcctc tcgggcgtca gctcggcctg cggcaggtgg
     4021 cgcgcggcgc cgcggctggc catgtattgc gcgttgtcgc gctggtgcga ggtggtgctg
     4081 atcacgagcg gcacgaggat gctggcgacg cccgcggcgc acagctcgct gaccgtgatc
     4141 gcgccggcgc ggcagacgat caggtcgcac tcggccaggc gccgcggcat gtcgtcgatg
     4201 aacggcacga cctcggcctc gacgccgcgc gccaggtatt cggcctgcac cagttcccag
     4261 ttcttctcgc ccgtctggtg cgtgacgcgc ggacgctcgc ccgcggggat cagcttgagg
     4321 gcctgcggca gcgtctcgtt gagcaccttg gcgcccaggc tgccgccgac caccagcacg
     4381 cgcagcgggc cggcgcgctg cgccaggcgt tcggccggcg gcgacagggc ctcgatctcc
     4441 gcgcgcaccg ggttgccggt gaccagcgcc ttgggcgtgg cgcgcgcatc gtcgccgtcg
     4501 aagccgaagg cgatgcggtc gcacgacgac agcagcgact tgttggacag cagcagcgcc
     4561 gcgtcggcgt tgaccagcac cagcggcttg cgcaggcgcc gcgcgacctt gccgcccggc
     4621 acgcagacgt agccgcccat gcccagcacc gccgacgcgt tgcgggtgcg caggatgtgc
     4681 ctgcacgcgc cgaaggccgc cgcgagctgc atcaggccct tgaacgtgcc cagcaggccc
     4741 ttgccgcgca cgccggcgaa atcgatggtg tccatcggga tgcccgacgg cggcacgagg
     4801 cggttctcca tgccggtgcg cgtgcccagc cagctgacgg tccagccgcg cgcgagcacc
     4861 tcgcgggcga cggcgaggcc cggcatcacg tggccgccgg tgcccgcggc catcacgacg
     4921 aggtggcgcg gcgcgcgcgt ggccaggtcg gaactcatgc ccgccctccc cgcatcagcg
     4981 cacggctttc catgtcgacg cgcagcacca gcgcgagcgc gacgaggttg agcaccaccc
     5041 ccgagccgcc gtagctcagc agcggcagcg tcaggccctt ggtgggcagc acgccgaggt
     5101 tcacgccgaa gttgatgaac gactgcgcgc ccatccacag gcccacgccc tggcacatca
     5161 ggcccgcgaa cacgcggtcc agcgcgatcg cctggcggcc gatgatgaac agccggcgcg
     5221 tgagccagaa gaacgcggcc acgatgatga acacgccgac gaagcccagc tcctcgccga
     5281 ccacggccag caggaagtcg gtgtgcgcct cgggcaggta gtgcagcttc tcgatggagt
     5341 tgcccaggcc ctggccgaag atctcgcccc ggccgaacgc gatcagcgag tgggtcagct
     5401 ggtaggcctt gccctgcgcg taggtcgggt tccacgggtc gaggtacgcg aagatgcgct
     5461 ggcggcggaa ctcgctgaac acgatcatca gcacgaacgc gcccagcagc acggcgacgc
     5521 tgagcaggaa catgcggccg ttgacgccgc ccaggaacag cacccccagc gcgatggcgg
     5581 cgatcaccat gaacgcgccc atgtcgggct cggccagcag caggacgccg atcacgccga
     5641 gcgcgaccgc catcggccac acggccttga tgaagttctc cttcacgtcc atcttgcgca
     5701 ccatgtagct ggccgcgtac atgcacatgc ccagcttggc gagctcgctg ggctggaagt
     5761 tcagaacgcc cagcgggatc cagcggcgag cgccgttgac gcccttgccc acgtgcggga
     5821 tcagcaccag cgccagcagc accatcgcca gcgcgaagat ccacggcgcc cacttctccc
     5881 agatcgcgat cggcacctgc gcggtggcga aggcgcacac ggagccgatg gcgatgaaca
     5941 tcgcctggcg gcccaggaag tgcgtggacg aatacagcgc gaagcgcggg ctgtcgggca
     6001 gggccaccga cgccgaatag accatcacca ggccgaacgc cagcagcgcg gccgtcatcc
     6061 agatcagcgc ggagtccacg cccatgacgc gcgtgggccg gcccgcgtcg acccagtcgc
     6121 ggatcggcac gcgctcgtcg ccggcggcgg ccgcgcggcg cttgcggaac cactcggcgg
     6181 gccggaagcc cggccactcg ggcaggtgct gcttgagcag ggccatcacg ctcatgccag
     6241 gacctccccg gcatcgtcgg caatgtcatg caccgtggcg acgaacacgt cggcgcggtg
     6301 cttgtagtcc cggaacatgt ccaggctcgc gcaggccggg ctcagcagca cggcatcgga
     6361 gccgtgggcc tgctggtgcg cccagcgcgt ggcctcttcc agcgtcgcga agcgctcgaa
     6421 cggcacgctg gccgtgcgca gcaccgcctc gagggcctcg gcgtccctgc cgatggtcgc
     6481 cgcggcgcgg gcatggcggc gcagcggctc ggtcagcggc acgaagtcct ggcccttgcc
     6541 gtcgccgccg aggatcacga ccagcttcga aggcgcgcga tcctggccca ggccgttcag
     6601 cgcggcgacg gtggcgccga cgttggtgcc cttggaatcg tcgaagaact cgacctgccc
     6661 gatgatgccc accggctcga cgcgatgcgg ctcgccgcgg tattcgcgca ggccgtgcag
     6721 catcggcgcg agcgggatgc cgaccgagct cgcgagcgcc agcgccgcca gcgcgttgga
     6781 tgcgttgtgg cggccgcgga tgcgcagcgc gtcggcgggc atgaggcgct ggatcacgag
     6841 gtcgatctcg tcgccgggct tgcgcttgat cgtcgggtcg agctccatcg cgcggacgag
     6901 ccaggccatg ccggcctcct ccacgaggcc gaagtcgccg ggctcgcgcg gcgcgtccag
     6961 gccgaagcgc acgacgcgcc gatggacctg cttcgcacgc tcgccgcggc gggtcggctt
     7021 gacgatgacc ggcggcggga ccatcgcctc gactgcaggg tcgtcgcggt tgatgaccat
     7081 gaccgtggcg ggcatcgcgt cggtgccgaa gatgcgcgcc ttcgcagcgg cgtacgcctt
     7141 catcgagccg tgccagtcca gatggtcctg cgtgatgttc aggacggtgg cggcgctcgg
     7201 gttgaagtcg tgcacctcgt ccagctggaa gctcgaaagc tccagcaccc acacctgcgg
     7261 caggtggctg aacaccgggt cggcgggcgg cggcgggatc agcgtgatcg ccggggcgtc
     7321 gtcgtcgggc agcgtggccg ccgcttccgc cagctccgcg ctgtcctcgg ccagttgctc
     7381 gtcggcatgc gcctcgtcga cgtcctcggc cagttgcgcc ggcacgtcgc tgccgtcgag
     7441 cgcggcgagc tcgcgcagct cgtcggcatc ctcggtctcg acgagctcgc gcgactgggc
     7501 gtcggccagg ggcgtgtcgg catcgacggg cacggcctcg gccgccgcga cgtcagtggg
     7561 cacctcggcc tcgaccggca tctcgagttc ggcgtccgga ttcgccttgg ccgcggcctt
     7621 cgcggcgcgc gcctgcgcac gcgccgcctt cgccgcggcg gcctcggccc tggcctgcgc
     7681 cttcgcgtcc tcctcggcct gcctggcggc agccgcggcg gcgagctcgg cgtcgagtcg
     7741 cgcctgcttc tccgcggcca cgcgcgcggc ctccgcgtcg atggcatcct gcgcggccct
     7801 ggcggcggct tcctcggcgt cgagcagcgc ctgcgcggcg gcggcctccg cggcctcgac
     7861 gtcggcgacg gcctgcgccg cgatcgcggc ggccagggtg tcgagcatcg tcgggccgat
     7921 gttgccggca acggccacgc tgcgtcccgc gcgctcgacg agttggcccg tcagcgaggt
     7981 ggtcgtcgtc ttgccgttgg tgccggtgat ggccaggatc ttcggcgcgt agccgcgctc
     8041 ggccttcagc tgccccagcg cgaccgagaa caggtcgagc tcgccacgca cgggcacgcc
     8101 ttcatccctg gcggcctgca gcagcgtcag cagccgcggg tcggcggggc tcaggcccgg
     8161 gctcttgtag acgcgggcga cgccgtccag cgccgcggcg gtgagctcgc ccgagaagaa
     8221 cgtgctgcct tcgacctcgg cagccagcgc cgcggcctgc ggcgggttcg gacgcgaatc
     8281 ccacacgcgg acgcgcgcac cctggcctgc gcaccagcgc gccatggcca gacccgaggc
     8341 gccgaggccc aggatcagca cggtgttggg agactgctcg ctcatgatct caacgcagct
     8401 tcaggctcgc cagcccgacc aggcacaaca gcatcgtgac gatccagaag cggatcacga
     8461 cctgcgtctc ggtccagccg gacttctcga aatggtggtg cagcggcgcc atcttcagga
     8521 tgcgccgccc ttcgccgtac ttcttcttcg tgtacttgaa gtagctgacc tgcgccatca
     8581 ccgacagcgc ctcggcgacg aacacgccgc ccatgatccc cagcacgatc tcctggcgcg
     8641 tgatcacggc gatcgtgccg agcgcgccgc cgagcgccag cgcgccgacg tcgcccatga
     8701 acacctgcgc cggatgcgcg ttgaaccaca ggaacgcgag ccccgcgccg gcgatggccg
     8761 cgcagaagat cagcagctcg ccggcgcccg ggatgtacgg gaacagcagg tacctggagt
     8821 agacggcgtt gccgaccacg tacgcgaaca gccccagcgc gccggccacc aggacgaccg
     8881 gcatgatggc caggccgtcg aggccgtcgg tgaggttgac cgcgttgctc gcgcccacga
     8941 tcacgaagta cgtcatcgcg atgaagccga acacgcccag cgggtagctc accgtcttga
     9001 agaagggcag catcaggtcg gccttgggcg gcaggtcgtt ggcgaagccg ctcgtgaccc
     9061 agcgcaggaa cagctccagc acgcgcaggt tggacgtctc ggacacgctg aacgcgaggt
     9121 acagcgccgc gaccaggccg atcagcgact gccacatgta cttctcgcgc gagggcatgc
     9181 cctccggatt cttctgcacg accttgcgcc agtcgtccga ccagccgatc gcgccgaagc
     9241 cgaaggtcac cagcatcacg atccagacga agcggttgct ccagtcgctc cacagcagcg
     9301 tggcgatggc gatcgcgatc aggaccagca cgccgcccat cgtgggcgtg ccgcgcttga
     9361 ccatgtgcga ctgcacgccg tattcgcgga tcggctggcc gatcttcagc tcggtcaggc
     9421 gccggatgac ccacggcccg aagaacaggc cgatcagcag cgccgtcatc gcggccatca
     9481 ccgcgcggaa ggtgtggtac tggaacacgc gcaggaagcc gaagctctgc gggtagtgct
     9541 cctgcagcca ggacgtgagg ctaagcagca tgggcgcctc cgtcgtcgtg cgattgcagc
     9601 agcgccacca cgcgttccat gcccatgaag cgcgagccct tggccagcac cgacgcgaac
     9661 gccggctcgc tgtcccgtgc gtcgccgagc gcggccagca gcgcgggcgt gtcgtcgaag
     9721 tggcgcgcat cgggcccgta ggcgcgcgcg gcgtgggcgc acagcccgcc ggcgcaccag
     9781 aactcgtcga tgccgcgcgc cttcgcgtag gcgccgatct cctggtggaa ggccggcccc
     9841 tggtcgccga cctcgcccat gtcgcccagc agcagccagc gcggcgcggg cagcccggcc
     9901 agcacgtcga tggcggcgcg cacggagtcg gggttggcgt tgtaggtgtc gtcgaccagc
     9961 gggatcgtcc tgccgccgcg ctggagcgac ttcagctgcg agcggccctt gaccggcgcg
    10021 aacgactcca ggccgcgcac gacgtcggcc agcgcgatgc ccgcgccgag cgccgccgcg
    10081 gtcgacgcga gcgcattgcg cacgttgtgg cgcccggcca cgcgcagcgc caggcgcgcc
    10141 tgtccctcgg gcgtggtcat ctcgacttcc caggcatcgc cgacccagcg cgcgcgggcc
    10201 gagatctcgg cgcgcggcca cgggccctcg gcctcgccgg ccagcgcgaa gcgcaggcag
    10261 cggcgcccgg cggcctgcgc ctgccagatg tcggcgtact cgtcgtcgca tgggaagacg
    10321 gcgacgccgt ccgccggcag ggcctcgagc gcggcgccgt tctcgcgcgc caccgcctcg
    10381 acggtgccca tgaattcctg gtgctcgcgc tgcgcgttgt tgacgagcgc gacggtcggc
    10441 tgcgcgatgc gcgcgagcac ggcgatctcg cccggatggt tcatgcccag ctcgatgacc
    10501 gccgacttgt gcgaggcgtc catgcgcagc accgtcagcg gcacgccgat gtggttgttg
    10561 aggttgcccg ccgtcgcgtg cgccgcgtcg gcatgggcgg cgcgcaggat ggacgcgatc
    10621 atctgcgtga cggtggtctt gccgttgctg ccggtgacgg cgaccaccgg gcccgcgaag
    10681 cgccggcgcc atccggtggc cagggcctgc agcgcctgca gcgagtcggt gacgacgagc
    10741 ccgtcgagcc cggcctcggc cacgccgtgc gtgccgatcg ccgccaccgc gccggcggcg
    10801 cgcgcctgcg gcaggaagtc gtgcgcatcg aagcgctcgc cctggagcgc gacgaacagg
    10861 tcgcccgcgc gcagcgtgcg ggtgtccgtg tgcacacgct cgaacgcgcg cgcgccgtcg
    10921 ccgacgagca ccgcacccgg caccatcgcg acggcttcgg cgagcgtcat catccgtgtc
    10981 atgcggccag tccccgcttc ctgagtccgg ccagcgcttc ctcgacatcc gagaagtgct
    11041 gccgcacgcc gttgatctcc tggtagtcct cgtggccctt gccggccacg agcaccacgt
    11101 cgcgcgcgtc cgcgcccgcg agcacctgcg cgatggccgc gcggcggtcg acgatcacct
    11161 gcaccggcgc gccgcccgtc gcgcccgcag cgacctggtc gacgatcttg cgcggatcct
    11221 cggtgcgcgg gttgtcgctg gtgacgacga cggcgtcggc caggcgcgcg gcgatgccgc
    11281 ccatcagcgg gcgcttggtc gcgtcgcgat cgccgccgca gccgaacacg caatgcagtc
    11341 ggccgccgcg gtcggccgcg agcggacgca gcgcggcgat gaccttctcc agcgcgtcgg
    11401 gcgtgtgggc gtagtcgacc accacctcgg gctggcccgc ggcgttcgcg gcgacgcgct
    11461 gcatgcggcc cggcaccggc gtgacgaggc ccgcgacgcg cgcggcgtcc gccagcgtca
    11521 cgcccagcgc gcgcagtccg ccgatcacgg ccagcaggtt gtgcacgttg tagtcgccga
    11581 tcagcgtcgt ggcgacggcg gcgcgctccg tgccggactc gaccagcgtg aacgcgaggc
    11641 cgccgtcgcg gtagccgatg tcctcggcga ccaggcgcgc cggcgaatcg ccgcgcgtgg
    11701 aatacgtcca caagtcgagc gggcccttgg tgagctcgcc cgccagcagg tcgccggcgt
    11761 cgtcgtcgat gttgacgacg gccgccttca ggcccggcca gccgaacagc aggcgcttgg
    11821 cggcccagta gctgtccatg ctgccgtggt agtcgaggtg gtcgcgcgtg aagttggtga
    11881 acagcgcgac ctcgatgcgc gtgccggcca ggcgctgctc gacgatgccg atggagctgg
    11941 cctcaatcgc gcatgcggca aagccgtcgt cgaccatgcg gcggaacgcg gcctgcagcg
    12001 ccaccgggtc cggcgtggtc aggcccgtgg cccggatcgt ccagtcctgc ctggaatcgg
    12061 ccgcggcgac gcccatcggc agcggcggca cgccgatgcc cagcgtcccc acgacacccg
    12121 tgcgccggcc gagcaggttc agcgcctgcg acgtccacca cgcggtcgac gtcttgccgt
    12181 tggtgccggt ggtggccacc accttcaggc gctcgctcgg ctcgtcgaag aacgcggcgg
    12241 cgatgtgtcc ggtggcggcc ttcatcgacg tgagcgcggc gatgcgcgcg tcctcgaagc
    12301 cgaacgcgtc gacctggtcc tgctcgacga ggcacgtcga cgcgcccgcg gcgagtgcgc
    12361 tggcgacgaa gcggcggccg tcgttggcgt agccgggcca ggcgatgaag gcatcgcccg
    12421 gctggacggc gcggctgtcc gtgcgcagcg tgccggtgac ccaggacttg agccagcggg
    12481 cagcggcggc gggggacttg aggcgggtca gagccatcag cgacgcccgg ccgcccgaag
    12541 gcgctgatgc gccccctcgg ggggcagcga atggaatgag cgtgggggct tcatttctct
    12601 agttctcggg aatctggacg acgttggcct gcgtgatctg cgtgcgcacg tcgaggtcgg
    12661 gcgcgacgcc gagcagcggc agcgtctgcg acaccacctg gctgaacacc ggcgcggcga
    12721 cgtcgccgcc gtagtgcagt ccggccgtgg gttcgtccac catcacggca accacgatgc
    12781 gcgggtgcga gatcggcgcc aggccgacga accacgggcg gtacttgttc tgcgcgtagc
    12841 cgcggccctc ggccttgtac gcggtgccgg acttgccgcc gacgctgtag ccgagcgtgc
    12901 gcgccttcgg cgccgtgccg cccggcgacg cgaccatctg cagcatcgtg ttcagctcct
    12961 tgaccgtctc gggcttgaac acgcgcgggc ccgggccggg cgcgctctgg cgcagcatcg
    13021 acacgttctc gagcacgccg tcgttggcga acaccgtgta ggcgcgcgcc agctggaaca
    13081 gcgaggccga caggccgtag ccgtaggcca tcgtcgcctg ctcgatcggg cgccaggtct
    13141 tgtacggacg cagccggccg gtgacgacgc cggggaagtt gatctgcggc ttctggccca
    13201 ggccgatgga cgagaacttc tcccactctt ccttgggcgt gagcttcatc gccagccgcg
    13261 cggcgccgat gttgctggac ttctggatca cctgctgcac cgtcagccag ccgttgggct
    13321 cggcatcctt gggcgcgaag ccgttgatga ccagcgagcc gggcgtcgcg tcgaccatcg
    13381 acgtcggcgt gaccaggccc ttctcgagcg cggtgccgac gatgaacggc ttcatcgtcg
    13441 agccgggctc gaacacgtcg gtcagcgcgc ggttgcgcag ctgcgcgccc gacaggtggt
    13501 ggcggtcgcc cgggttgtag ctcgggtagt tggccagcgc gagcacctcg ccggtgagcg
    13561 tgtctatcac gaccacgctg ccgcccttgg ccttctgcgc gatgacctgg tcgcggatgc
    13621 gctggtacgc gaagaactgc accttggagt cgatcgacag ctcgatgtcc tcgccgttga
    13681 tcgggcgctg gggatcgccg atgtcctcga cgatgcggcc caggcgatcc ttgaccactt
    13741 cgcgctggcc gtcgcggccc tgcagctgct gctggtaggc gagctcgatg ccttcctggc
    13801 ccttgtcctg gtcgccggtg aagcccacca cgtgcgcggc ggcctcgccc tccggataac
    13861 ggcggcggaa ctcgctgacc tggtggatgc ccttcaggtc gagcgccttg acggccgcgc
    13921 cgacgctgtc gtcgacctgg cgcttgagcc acacgaagtt cgggttgccg tcgaggcggt
    13981 cgtcgagctc ggccaccgtc atgcccagcg ccttggcgag ctgcttcttc tcgcccgcgt
    14041 cggcgtcgac gtccttggga atcgcccaga tcgatggcgt gggcacgctc gtggccagca
    14101 gcaggccgtt gcggtcgacg atgcggccgc ggctggccgg catctcgagc gtgtgcgcgt
    14161 agcgcgcctc gccttgcttc aggtagaagt tggtggcgat gatctggatg taggcggcgc
    14221 ggcccaccag gatcagcgag ccgatgccta ccagcaccac caggaagcgg ctgcgccaca
    14281 gcggcgtctt ggacgccagc agcgggctgt tggcgtacgt gacgttgctg accgcgcggc
    14341 cgcgcggcgt ctggacgcgc ttgttggcct tgctcattgc gcggcccccg aatcgggcac
    14401 gctggccggc gccgccgtgc gcgcgagcgg cgccgactgc ggtgcggccg caagcccggc
    14461 gtcgaccacg tactgcgtgt tggccggcgt ggcggcgcgc atgttgagct tggtgcgcgc
    14521 ggtcgcctcc acgcgcaggt gcgtggcctg cgcctggcgc tcggcctcga ggcggcggcg
    14581 gtcggactcg agctggcgct cggtgccgtg cgcgttctcg atctcggcga acaggcggcg
    14641 cgactcgtag ctgatcttga cgaggtacag cccgctgacg atcagcgcga tcgccagcag
    14701 cacgttcagc cgcaccatga aggattggcc gctcatgcga gcctctccgc cacgcgcagg
    14761 atcgccgagc gcgcgcggcc gttgttcagc acctcggtcg gccccggctt gatgcggccc
    14821 agcgcgttca ggcgcagcgc cttggccggc ggcgcgaacg gcgcgcggcg gtcgatctcg
    14881 gccttgctct cgcgtgcgat gaaggtcttg acgatgcggt cttccagcga atggaagctg
    14941 atgacggcaa ggcggccgcc ttcttcgagc aggtccagcg acgcgttcag gacctgttcg
    15001 agctcctcaa gctcggcatt gacgaaaatc cgaagagcct gaaacgtgcg cgtggcggga
    15061 tcctggcccg cttcacgggt cttgaccgaa cgagccacga cggcggacag ctcgcgagtg
    15121 gttcgcacgg gcgttcctcc atcgcgcaga gccacaatcg cctttgcaat cgacacagca
    15181 aaccgttctt cgccgtagtc acggatcacc tctgcaatgt gacgctcgtc cgcgcgagcg
    15241 aggaagtcgg cggccgtctc gccgcg
//
LOCUS       DATCRO010000558         9856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_85019, whole genome shotgun sequence.
ACCESSION   DATCRO010000558 DATCRO010000000
VERSION     DATCRO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85019"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VIN75_15260"
     CDS             <1..1053
                     /locus_tag="VIN75_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HEY8975576.1"
                     /translation="VQGPAGETLLFDLQDKPWGPNYLRAGLDLHADFSGHSGFDVKLS
                     HNHHWLTENGTEWRNFIQVGERPAIFSEIYHPLFRNIDDGSDWFAAAYISGDRQHRTV
                     YQGEDGAELAQFLRSTGQIGLDLGLPFGRLGEARAGFLHLTTSTTPTLLSAQWTGPTQ
                     AQIWQEDGLRLRGVVDQLDYVNFPTSGYRLVGEGVVGTRRLQREHSEHMARGSVEGTA
                     VDTFGRSTFELHGALKMSDQHSLLVVDQYTLGGFQQLSGYRPDQLSGNAVLFGRLNWY
                     MRLANSPVFARGFYVGASVEAGNAWADRSEMSLAHLRGGGSVYLGADTGLGPMYLGVT
                     YAPRGELGVALFIGRP"
     gene            complement(1093..1566)
                     /locus_tag="VIN75_15265"
     CDS             complement(1093..1566)
                     /locus_tag="VIN75_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY8975577.1"
                     /translation="MTTTSTPTLYDWLGGIDALNRLTARFYEHVAHDALLAPVFAHMS
                     PEHPAHVAAFLGEVLGGPATYSAEHGGHPHMIRQHLNRHLTQDKRRRWVELLLDTADE
                     LAMPDDPEFRSALVGYLEWGTRLAVINSQPGATVDEHAAMPRWGWGEVKGPYTGG"
     gene            complement(1795..2526)
                     /gene="yihA"
                     /locus_tag="VIN75_15270"
     CDS             complement(1795..2526)
                     /gene="yihA"
                     /locus_tag="VIN75_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY8975578.1"
                     /translation="MVSDDAKEGLGWLHTAKFLTSAGKINQLPQGDMPEIAFVGRSNA
                     GKSSAINTLAQQRQLAFASKTPGRTQLINLFALGPREAPDAYLADLPGYGYAAVGRDK
                     KLVWQKVMADYLEVRRALTGVVLMVDSRHGFTPLDRQLMDFIAQRVRTGEVKLLVLLT
                     KADKLTKSESVAAVAAASTVLGEFSTEDSDIGVTLFSSLRRIGVEDAAEQLLAWKRAM
                     PERVHFELPPMAEGEAGEGVEGDEV"
     gene            2654..3280
                     /locus_tag="VIN75_15275"
     CDS             2654..3280
                     /locus_tag="VIN75_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8975579.1"
                     /translation="MKSLCVVALAALFATSVMADEAKGPAKPDLAKGETISKTVCAAC
                     HTADGSRGIPTNPILQGQHADYLVKQLTEFKAGKRDNAVMKPMASTLSEDDMRNVAAF
                     YQSKSAKAGQSKHKETVALGEKIWRGGIAEKSVPACAGCHGPAGAGVPAQYARLAGQH
                     AEYVSAELGLFRSGKRANSPQMATIAARMSDAEIAAVSDYVEGLNTAR"
     gene            3411..3743
                     /locus_tag="VIN75_15280"
     CDS             3411..3743
                     /locus_tag="VIN75_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975580.1"
                     /translation="MPFIRRNASGAIDSLHRRDEGGCEFVPDDHPDVRAFLGAAAPSH
                     DQNFATLDADFIRVIEDVIDTLISKNVINITDLPLEAQTKLFGRKGYRQRAAKDALKL
                     FGDDEAAI"
     gene            3791..4222
                     /locus_tag="VIN75_15285"
     CDS             3791..4222
                     /locus_tag="VIN75_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975581.1"
                     /translation="MQPSFTVMVAAIVAVVAAAGWMLLGSHSRHRRLERLRDARDAAN
                     AEARCVAAFPSVDPLSAHAAYRWVQALVEVENMPLHPDDGLEALLGIDPGKIDDKFDA
                     CREFAGAARPGLEVPRPLRTVRALMAAVLAAGCELDPADAA"
     gene            complement(4241..4927)
                     /locus_tag="VIN75_15290"
     CDS             complement(4241..4927)
                     /locus_tag="VIN75_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY8975582.1"
                     /translation="MTIPTLSLLETRILGVLVEKQHTVPDSYPLSLNALTLGCNQKTA
                     RDPVINASENDVLAALDTLKSQHLVLEGSGSRVPRFEHNVARVLKLPGQSVALLAMLM
                     LRGPQTAAELRLNTERLHRFADISSVEGFLDEMATHEPPFVVKLARAPGARESRWAHL
                     LSGPVADAAPAAPQSGPGTPLSDTALGMSEVAALRAEQLRMKGELERLRAQVARLANE
                     LGVDLDGGAA"
     gene            complement(5275..6966)
                     /gene="acpA"
                     /locus_tag="VIN75_15295"
     CDS             complement(5275..6966)
                     /gene="acpA"
                     /locus_tag="VIN75_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY8975583.1"
                     /translation="MKNRIKPIALLTPLALAAVLSLTACNDSDSPAPVDTTAALQSKV
                     QNIVVIYAENRSFDNLYGNFPGANGLSTVVDATGKTTAAYVPQVDRDGSALATLPPTW
                     GGVMMPGQSVTVTEAQSAGLPNAPFNIATAYKTSAGVTITGDMVTRDLYHRFFENQMQ
                     IDGGKNDKFVAWADAGSLVMGNFDYSASPLYKLAQQYTLADNFFQGAFGGSFLNHQYL
                     ICACAPTVDAATVTANKMTVNNVATNADGSFTTSLVPAASSASSVMTAKPTLQTGNIT
                     PLNYFGDNKYHAVNTMQAPFQPSGNGPVDVAGDDAKYANPTAATTLPPQTQTNIGDLL
                     TAKNINWKWYAGSWNSAVADGMQAPASKRTVIYAGDTNGVASTAAVDFQPHHQPFNYQ
                     ANMDPVTHAADRANHLKDYTDLVADAAAGTLPPVAFYKPEGLYNQHPGYANITGADQK
                     IADLVTKLQASPQWKNMVIVITYDENGGQWDHVAPPKGDKIGPGTRIPALIISPYSKT
                     GTVDHTQYDTASVLRLITRRFGLDTLAGLTARDTALKANGAPAMGDLTNALDLTK"
     gene            7152..8417
                     /locus_tag="VIN75_15300"
     CDS             7152..8417
                     /locus_tag="VIN75_15300"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8975584.1"
                     /translation="MNRAAPSLAKLILLVMFLAATALAHWVKATPAPAPRELPFYATR
                     FEQRPDAKTLTALGRALFSDPALSASGTMSCASCHDPAHAYGPANHLAVQLGGPDRNL
                     PGLRAVPSLKYHQTIQPFSERFFDNDGNDAADQGPTGGFDWDGRADSAHEQAAGPLLS
                     PFEMANPDKDAVVARLRASPNAAPLRAAFGAHVLDEPQRAWNALVLALEVFQQSPQDF
                     YPYDSKYDAFLRGQATLTPQEARGLAVFNDAGRGNCASCHVSAIKRGAFPQFTDAGLI
                     AIGVPRNPAIPANAKADFRDLGVCGPLRTDLKSHDDYCGLFRTPSLRNVALRGVFFHN
                     GVYSRLADAVRFYALRDVDPARIYGTGRFDDLPGKYQGNLNVEAPFGGKRGDKPRMTE
                     AEIADVVAFLGTLTDGWNAPRGGQVAQRP"
     gene            complement(8434..8958)
                     /locus_tag="VIN75_15305"
                     /pseudo
     CDS             complement(8434..8958)
                     /locus_tag="VIN75_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
     gene            complement(9008..9637)
                     /locus_tag="VIN75_15310"
     CDS             complement(9008..9637)
                     /locus_tag="VIN75_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8975585.1"
                     /translation="MELTLHIDKLVFAYGQRWLFEGFSARAGAGVTWVQGANGSGKST
                     LLRLLAGALAPRHGSFELAGCRYAAGDRVAPLAWRRQTFWGLSDPPPTPWLTIAECLG
                     FVAGTYGGIDAARLREQVDALGLAPALPTPLREASLGQQRKTLLALALALPVKLLLLD
                     EPFNALDVASAKHLSDTLAARAAQGGQVILLTSHVEPHVPVRDTWVLRG"
BASE COUNT         1382 a         3466 c         3538 g         1470 t
ORIGIN      
        1 gtgcagggtc ccgccggcga gacgctgctg ttcgacctgc aggacaagcc ctggggcccg
       61 aactacctgc gcgcgggcct ggacctgcac gccgacttca gcggccacag cggcttcgac
      121 gtcaagctga gccacaacca ccactggctc accgagaacg gcaccgagtg gcgcaacttc
      181 atccaggtgg gcgagcggcc ggcgatcttc tcggagatct accacccgct gttccgcaac
      241 atcgacgacg gctccgactg gttcgccgcg gcctacatct cgggcgaccg ccagcaccgc
      301 accgtctacc agggcgagga cggcgcggag ctcgcgcagt tcctgcgctc cacgggccag
      361 atcggcctgg acctgggcct gcccttcggc cgcctgggcg aggcgcgcgc cggcttcctg
      421 cacctgacga cgtcgacgac gccgacgctg ctgtcggccc agtggacggg cccgacgcag
      481 gcgcagatct ggcaggagga cggcctgcgc ctgcgcggcg tggtcgacca gctggactac
      541 gtcaacttcc ccaccagcgg ctaccgcctc gtcggcgagg gcgtggtggg cacgcggcgg
      601 ctgcagcgcg agcacagcga gcacatggcg cgaggctcgg tggagggcac cgcggtcgac
      661 accttcggcc gcagcacctt cgagctgcac ggcgcactga agatgtccga ccagcactcg
      721 ctgctggtcg tggaccagta caccctcggc ggcttccagc agctctcggg ctaccgcccc
      781 gaccagctct ccggcaacgc cgtgctgttc ggccgcctga actggtacat gcgcctcgcc
      841 aactcgccgg tgttcgcgcg cggcttctac gtcggcgcgt cggtggaggc cggcaacgcc
      901 tgggccgacc gctccgagat gagcctggcg cacctgcgcg gcggcggcag cgtctacctc
      961 ggcgcggaca cggggctcgg gccgatgtac ctgggcgtga cgtatgcgcc gcggggcgag
     1021 ctgggggtgg cgttgttcat cgggcggccg tagcgcgcgg atcatcggcg cacgcgcgcg
     1081 ccgaccgcca ggtcagccac cggtgtacgg ccccttgacc tcgccccagc cccacctggg
     1141 catggccgcg tgctcgtcga ccgtcgcgcc gggctgcgag ttgatgactg ccaggcgcgt
     1201 gccccactcg aggtagccca ccagcgcgga gcggaattcc gggtcgtcgg gcatcgcgag
     1261 ttcgtcggcg gtgtccagca gcagctcgac ccagcggcgg cgcttgtcct gcgtcaggtg
     1321 gcggttcagg tgctggcgga tcatgtgcgg gtggccgcca tgctcggccg aataggtcgc
     1381 cgggccgccg agcacctcgc ccaggaacgc cgcgacatgc gccggatgct ccgggctcat
     1441 gtgcgcgaac acgggcgcga gcagcgcatc gtgcgcgaca tgctcgtaga agcgcgcggt
     1501 gagccggttc agcgcgtcga tgccgcccag ccagtcgtag agcgtgggcg tggaggtcgt
     1561 cgtcatgcga cgcattctgc gcggattgcg gcaggcgcgc gcgagtcggg gtcaggtctt
     1621 gcgcgagtcg gggtcaagtc ttgaatcgtt gcgtgccggc acctcgccgg agggtgtccg
     1681 ccgtaggggg tcaggtcttg aaagttgcgc ggtggaactt ccgcggtcgg cgagcggcac
     1741 cgcgcgcaac tttcaagacc tgaccccctc cttcacaaga cctgaccccc tccttcagac
     1801 ctcgtcgccc tcgacgccct cgcccgcttc gccctcggcc atcggcggca gctcgaagtg
     1861 gacccgctcg ggcatcgccc tcttccaggc cagcagctgt tcggccgcgt cctccacgcc
     1921 gatgcggcgc agcgacgaga acagcgtcac gccgatgtcg gagtcctcgg tcgagaactc
     1981 gcccagcacc gtgctggcgg cggcaacggc cgccaccgac tcgctcttcg tgagcttgtc
     2041 ggccttggtc agcagcacca gcagcttgac ctcgccggtg cgcacgcgct gggcgatgaa
     2101 gtccatcagc tggcggtcca gcggcgtgaa gccgtggcgg ctgtcgacca tcaggacgac
     2161 gcccgtcagc gcgcgccgca cctcgaggta gtcggccatc accttctgcc agaccagctt
     2221 cttgtcgcgg ccgacggcgg cgtagccgta gccgggcagg tcggccaggt aggcgtcggg
     2281 cgcctcgcgc gggccgagcg cgaacaggtt gatcagctgc gtgcggcccg gcgtcttgga
     2341 cgcgaacgcg agctggcgct gctgggccag cgtgttgatc gccgacgact tgccggcgtt
     2401 gcttcggccc acgaacgcga tctcgggcat gtcgccctgc ggcagctggt tgatcttgcc
     2461 tgcggaggtc aggaacttgg cggtgtgcag ccagcccagg ccttccttgg cgtcgtccga
     2521 gaccacgacc gccttctggt gggcgggtgt cgcgggggat gagggtttgc gcgggggcct
     2581 gggatgggtc atggtgcatt gtaaaatcgc tgggtttgct ccatccaaaa agcctcttga
     2641 agggttcgta gagatgaagt ccttgtgcgt tgttgccctg gcggccctgt tcgccacctc
     2701 cgtgatggcc gacgaagcga aggggccggc caagccggac ctggccaagg gggagacgat
     2761 ctcgaagacg gtctgcgccg cctgccacac cgccgacggc tcccgcggca tcccgaccaa
     2821 cccgatcctt cagggtcagc acgccgacta cctggtcaag cagctcaccg agttcaaggc
     2881 cggcaagcgc gacaacgcgg tgatgaagcc gatggcgtcg acgctgtccg aagacgacat
     2941 gcgcaatgtc gccgcgttct accagtccaa gtcggccaag gccggccagt ccaagcacaa
     3001 ggagacggtg gcgctgggcg agaagatctg gcgcggcggc atcgccgaga agtcggtgcc
     3061 cgcctgcgcc ggctgccacg gccccgccgg cgccggcgtc cccgcgcagt acgcgcgcct
     3121 ggccggccag cacgccgagt acgtcagcgc cgagctcggc ctgttccgct ccggcaagcg
     3181 cgccaacagc ccgcagatgg ccaccatcgc cgcgcgcatg agcgatgcgg agatcgcggc
     3241 ggtctcggac tacgtcgaag gcctgaacac ggccaggtga ggcggggcgc cccggcgccc
     3301 tcgcctcgcg gcgcccaatg ccggacgccg cgcgtccctg cgtgaattcc tgctgcccgc
     3361 cccgcgcacg gtcgcgcacc gcgcgcgggc gagctcgcta tgatgggccg atgcccttca
     3421 tccgccgcaa cgcttccgga gccatcgaca gcctgcatcg acgcgacgag ggcggctgcg
     3481 agttcgtgcc cgacgaccat cccgacgtgc gcgccttcct gggcgccgcc gcgccctcgc
     3541 acgaccagaa cttcgcgacg ctggacgccg acttcatccg cgtgatcgag gacgtgatcg
     3601 acacgctgat ctccaagaac gtcatcaaca tcaccgacct gcccctggag gcccagacca
     3661 agctgttcgg ccgcaagggc taccggcagc gcgcggccaa ggacgcgctc aagctgttcg
     3721 gggacgacga ggcggcgatc taggagtctg cgcggcagaa gccgcgcaga ctcctagctg
     3781 gcgcaaccgc atgcagccga gcttcaccgt gatggtggcg gccatcgtcg cggtcgtcgc
     3841 ggccgccggc tggatgctcc tgggcagcca ttcccgccat cgccgcctcg agcgcctgcg
     3901 cgacgcgcgc gacgccgcaa acgctgaggc ccgctgcgtg gccgccttcc cgtcggtcga
     3961 tcccctgagc gcgcacgcgg cctatcgctg ggtgcaggcg ctggtggagg tcgagaacat
     4021 gccgctgcac ccggacgacg gcctcgaggc gctgctgggc atcgacccgg gcaagatcga
     4081 cgacaagttc gatgcctgcc gcgagttcgc aggggcggcg cgccccggcc tggaggttcc
     4141 ccgcccgctg cgcacggtgc gcgcgctgat ggcggcggtg ctggcggccg gctgcgagct
     4201 cgatccggcc gacgcggcgt gacgccgccg ggcgcgaccg tcaggccgcg ccgccgtcca
     4261 gatcgacgcc cagttcgttc gcgagccgcg cgacctgcgc gcgcagccgc tccagctcgc
     4321 ccttcatgcg cagttgctcg gcccgcaggg cggccacctc ggacatgccc agggccgtat
     4381 cggacagggg cgtgcccggg cccgactgcg gcgccgcggg cgcggcgtcg gcgaccgggc
     4441 cggacagcag gtgcgcccag cgcgactcgc gcgcgcccgg cgcgcgggca agcttgacga
     4501 cgaagggcgg ctcgtgcgtg gccatctcgt ccaggaagcc ctcgaccgac gagatgtcgg
     4561 cgaagcgatg cagtcgctcg gtgttcagcc gcagctcggc cgccgtctgc gggccgcgca
     4621 gcatcagcat cgccagcagc gcgacgctct gtccgggcag cttgagcacg cgcgccacgt
     4681 tgtgctcgaa gcgcggcacg cggctgccgg agccctcgag caccaggtgc tgcgacttga
     4741 gcgtgtcgag cgcggccagc acgtcgttct cgctcgcgtt gatgaccggg tcgcgcgccg
     4801 tcttctggtt gcagccgagc gtgagcgcgt tcaggctcag cgggtagctg tcgggcacgg
     4861 tgtgctgctt ctcgacgagc acgccgagga tgcgggtctc caggagcgag agcgtgggga
     4921 tggtcatgca ccggagtatc gctccgccgg gcggggcgac gcgatggcgg tgcgtccagc
     4981 gacgcagaaa cagcacatcg cgcgcggcga cgacgacgac gacgacgcct cgcaccaagc
     5041 gccaaccccg tcatcctgcg cgcagcgaag cgaagtcgca ggatccactt cggcgacgcc
     5101 acctcgcaac caccgtggat cctgcgactt cgcgcaggat accgggtggg cgcgtcgcgg
     5161 caacagggcc tcgggtgaga tggcgacaag gcatcaagcg aagcgcaccg cgcatgaaaa
     5221 gcgccccgcg ggcccgaagg ccggcggggc ggaacaggcg aagcttgctt gttcttactt
     5281 ggtcaggtcc agcgcgttgg tcaggtcgcc catcgccgga gcgccgttgg ccttgagcgc
     5341 ggtgtcgcgc gccgtcaggc cggccagcgt gtccaggccg aagcggcgcg tgatcaggcg
     5401 cagcaccgag gcggtgtcgt actgcgtgtg gtcgacggtg ccggtcttcg agtacggcga
     5461 gatgatcagc gccgggatgc gggtgcccgg gccgatcttg tcgcccttgg gcggcgcgac
     5521 gtggtcccac tggccgccgt tctcgtcgta cgtgatgacg atgaccatgt tcttccactg
     5581 cgggctggcc tgcagcttgg tgacgaggtc ggcgatcttc tggtcggcgc cggtgatgtt
     5641 ggcgtagccg gggtgctggt tgtacaggcc ctcgggcttg tagaacgcga ccggcggcag
     5701 cgtgccggcg gcggcgtcgg ccaccaggtc ggtgtagtcc ttcaggtggt tcgcgcggtc
     5761 ggcggcatgc gtcaccgggt ccatgttcgc ctggtagttg aaaggctggt ggtgcggctg
     5821 gaagtcgacg gcggcggtgg aggcgacgcc gttggtatcg cccgcgtaga tcacggtgcg
     5881 cttggaggcc ggggcctgca tgccgtcggc gaccgccgag ttccacgagc cggcgtacca
     5941 cttccagttg atgttcttgg ccgtcagcag gtcgccgatg ttggtctgcg tctgcggcgg
     6001 cagcgtggtg gcggccgtcg ggttcgcgta cttggcgtcg tcgccagcca cgtcgaccgg
     6061 gccgttgccg ctgggctgga acggcgcctg catcgtgttg acggcgtggt acttgttgtc
     6121 gccgaagtag ttcagcggcg tgatgttgcc ggtctgcagg gtcggcttgg ccgtcatcac
     6181 cgatgaggcc gacgaggcgg ccggcaccag gctcgtggtg aagctgccgt cggcgttggt
     6241 ggcgacgttg ttgaccgtca tcttgttggc cgtcaccgtc gcggcgtcga ccgtcggcgc
     6301 gcaggcgcag atcaggtatt ggtggttcag gaacgaaccg ccgaacgcgc cctggaagaa
     6361 gttgtcggcc agcgtgtact gctgcgccag cttgtacagc ggcgaggcgc tgtagtcgaa
     6421 gttgcccatg accaggctgc cggcgtcggc ccaggcgacg aacttgtcgt tcttgccgcc
     6481 gtcgatctgc atctggttct cgaagaagcg gtggtacagg tcgcgcgtga ccatgtcgcc
     6541 cgtgatcgtg acgccggcgc tcgtcttgta cgcggtggcg atgttgaacg gcgcgttggg
     6601 caggccggcg ctctgcgcct cggtcacggt gacgctctgg ccgggcatca tcacgccgcc
     6661 ccaggtgggc ggcagcgtcg ccagcgcgga gccgtcgcgg tcgacctgcg ggacgtacgc
     6721 ggccgtcgtc ttgccggtgg cgtcgaccac ggtagacagg ccgttggcgc cggggaagtt
     6781 gccgtacagg ttgtcgaacg agcggttctc ggcgtagatg acgacgatgt tctgcacctt
     6841 gctctgcagc gcggcggtgg tgtcgacggg tgccggcgag tcgctgtcgt tgcaggcggt
     6901 caggctcagc acggcggcga gggccagcgg ggtcagcagg gcgatgggct tgatgcggtt
     6961 cttcattggt ttgggcctcc agggcacgcg acggatgacg tagcccgaca gcatcgcgat
     7021 gccgtgtgac agccctggga aagcaagtgc aatccgggcg tcacactcgg atgaagcagg
     7081 gttgtcacgc gcgcgccaca cggggtcgct agcctgcccg ctccgtcgtc gacctgttct
     7141 gcagcttccc cgtgaaccgt gccgctccct cgcttgccaa gctgatcctg ctcgtgatgt
     7201 tcctggccgc gacggcgctc gcccactggg tcaaggccac cccggcgccg gctccgcgcg
     7261 agctgccgtt ctatgcgacg aggttcgagc agcgccccga cgcgaagacg ctcactgcgc
     7321 tcggccgcgc gttgttttcc gatccggcgc tgtcggcctc gggaacgatg tcgtgcgcga
     7381 gctgccacga tccggcgcac gcgtacggcc ccgccaacca cctggccgtg caactgggcg
     7441 gccccgaccg caacctgccc ggcctgcgcg cggtgccgtc gctgaagtac caccagacga
     7501 tccagccgtt ctccgagcgc ttcttcgaca acgacggcaa cgacgccgcc gaccagggcc
     7561 ccaccggcgg cttcgactgg gacggccgcg ccgactccgc gcacgaacag gccgcgggcc
     7621 cgctgctgtc gccgttcgag atggccaacc ccgacaagga cgccgtcgtc gcgcggctgc
     7681 gcgcctcgcc caacgccgcg ccgctgcgcg cggccttcgg ggcgcacgtg ctcgatgagc
     7741 cgcaacgcgc ctggaacgcg ctcgtgctcg cgctcgaggt gttccagcag agcccgcagg
     7801 acttctatcc gtacgacagc aagtacgacg ccttcctgcg cggccaggcg acgctcacgc
     7861 cgcaggaggc gcgcggcctg gcggtgttca acgacgccgg caggggcaac tgcgcgtcgt
     7921 gccacgtcag cgcgatcaag cgcggtgcgt tcccgcagtt caccgatgcc ggcctgatcg
     7981 cgatcggcgt gccgcgcaac cccgcgattc ccgcgaacgc gaaggcggac ttccgcgacc
     8041 tgggcgtctg cggccccctg cgcaccgacc tgaagtcgca cgacgactac tgcggcctgt
     8101 tccgcacgcc gtcgctgcgc aacgtggccc tgcgcggcgt gttcttccac aacggcgtct
     8161 actcgaggct cgcggacgcc gtgcgcttct acgcgctgcg cgacgtcgac ccggcgcgga
     8221 tctacggcac gggccgcttc gacgacctgc ccggcaagta ccagggcaac ctcaacgtcg
     8281 aggccccgtt cggcggcaag cgcggcgaca agccgcgcat gaccgaggcg gagatcgccg
     8341 acgtcgtggc gttcctgggc acgctgacgg acggctggaa cgcgccgcgg ggcgggcagg
     8401 tggcgcagcg accctgacgt cgcccccgcc ggctcactcg atcaggatcg cgcggaagtc
     8461 gttgacgttg gtcagcgtcg gcccggtgac cagcgagtcg gccagcgcgc cgaagaagcc
     8521 gcggccgccg ttgtcgtcga ggcgctcgcg cgggcgcagt ccggtggccc atgcgcgtgc
     8581 gagcgcgtcg ggcgccagcc aggcgccggc gatctcctcc tggccgtcga cgccgtcggt
     8641 gtcggccgcg agcgcgtgca cgcccggctc gccacccagc gcgatgccca gcgacagcag
     8701 gaactccacg ttgcggccgc cgcggccgcg gccgcgcacg gtcaccgtcg tctcgccgcc
     8761 ggacagcagc acgcagggcc gcgcgaaggg ctggccgcgg cgcgcgacct gcagcgcgat
     8821 gccggccatc gccttggcca cgtcgcgcgc ctcgccctcg atcgcgtcgc cgaggatgtg
     8881 ggtggtgtag cccgcctcgc atgccacgcg ggccgcggcc tcgggcgcga gctgcggcgt
     8941 cgcgatgaag cgggtctcga agcgtcatcc tgcgcgcagt cgcaggatcc acgccggccg
     9001 gccgcaccta cccccgcagc acccacgtgt cgcgcaccgg cacgtgcggc tcgacgtggc
     9061 tggtcagcag gatcacctgc ccgccctgcg cggcgcgcgc ggccagcgtg tcggacaggt
     9121 gcttcgccga cgcgacgtcg agcgcgttga agggctcgtc cagcaacagc agcttcaccg
     9181 gcagggccag cgcgagcgcg agcagcgtct tgcgttgctg gcccagcgag gcctcgcgca
     9241 gcggcgtggg cagcgccggc gccaggccca gcgcatcgac ctgctcgcgc aggcgcgccg
     9301 catcgatgcc gccgtaggtg cccgcgacga agcccaggca ttccgcgatg gtgagccagg
     9361 gcgtgggcgg cgggtcgctg agtccccaga aggtctgccg gcgccatgcg agcggcgcga
     9421 cgcggtcgcc cgcggcgtag cggcagccgg ccagctcgaa cgacccgtgg cgcggcgcga
     9481 gcgcgccggc cagcaggcgc agcagcgtcg acttgccgct gccgttggcg ccctgcaccc
     9541 acgtgacgcc ggcgccggcc cgcgccgaga agccctcgaa cagccagcgc tggccgtagg
     9601 cgaagacgag cttgtcgatg tgcagggtca gttccatgaa gtcctcttcg cggccgtcgg
     9661 aagtgttcga tgccgcctca gtcccacaac tcgctcccga acgccgtcaa cagcgcgacg
     9721 ctgatcgccc acagcgcgac gtgcgcctcg cggttggacg cgggcaccga ggtgagcgcg
     9781 gccggcgtcg cgaccaggcc gagcgcgaag gcggcgagcg gcccggcgcg ccacggacgc
     9841 tcgacgaccg ccagcg
//
LOCUS       DATCRO010000559         8230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1007479, whole genome shotgun sequence.
ACCESSION   DATCRO010000559 DATCRO010000000
VERSION     DATCRO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007479"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1014)
                     /gene="asd"
                     /locus_tag="VIN75_15315"
     CDS             complement(94..1014)
                     /gene="asd"
                     /locus_tag="VIN75_15315"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517196.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY8975586.1"
                     /translation="MKKQFRKLLAQEDLNFLLTNRVPRIALTHFMGKFSKIRNPIVSK
                     ASIGVWKLFTDLDLSDARTRKFRSMHDCFTRELMPGARPVDGDPGVLTSPCDAIVGAC
                     GPVSGTTVFQAKGFPYRMEDLFGPGTDTSPFRDGRFITLRLTSAMYHRFHAPHDVDVN
                     HVSYITGDTWNVNPIALRRVERLFCRNERAVLSARLRRGNHPIAIVPVAAILVASIRL
                     KFTDVLLHLKYRGPNEIPVEARFGKGEEMGGFQHGSTIIMFVPPGFEFVDGIATGARI
                     RMGQALMRLPAVAAGVDASGSREALATAQA"
     gene            complement(1021..2679)
                     /locus_tag="VIN75_15320"
     CDS             complement(1021..2679)
                     /locus_tag="VIN75_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8975587.1"
                     /translation="MEPSTLAFCALLLAALAVLAPRIKRRLELSRAKHRSLTGHSKMA
                     KRVAGFLPGYAYDEARFFGSDGAPQEFQQRRRAGFAGLVALYTERFARSAALTAQASS
                     GISDLQFTGSYRVPFQYSPYLRQHLKTGSFLQSSSGVTVTDLDGNTFHDLTGSYGVNL
                     FGYDFYKECIAEGAARVADLGPVLGAYHPVLLDNVRHLQRISGLDEVSFHMSGTEAVM
                     QAVRLARYHTKKKFLVRFCGAYHGWWEDVQPGPGNPLPPRETYTLEEMKAGTLKVLRT
                     RQDIACVLVNPLQALHPNAAAPGDSSLVDSARRAKFDRAAYTEWLRQLRQVCTERGIV
                     LIFDEIFIGFRLAPGGAQEYFGIQADMVTYGKSLGGGLPVGVVCGRRDLMKRFREDRP
                     ADICFARGTFNSHPYVMGAMNVFLERLARPDVRAMYDGVDERWNQRAARFNERLAAAG
                     VPVRVANLQSIWTVLYEQPSRYNWMLQFYLRAHGLALSWVGTGRLIFSLDYGDADFDA
                     VIDRFVAAAVQMQGDGWWWNDPAQSNRTIRRGLLREMLRERFSS"
     gene            complement(2703..3323)
                     /locus_tag="VIN75_15325"
     CDS             complement(2703..3323)
                     /locus_tag="VIN75_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975588.1"
                     /translation="MNTFMQTLRTLRWDDHRFYHHSTINQSLHLVSAIVFCISYGLLL
                     IDPAIAAVLAWTVSMTTRQAGHFFFEPRGYDHVNQVSDEYKEEVKAGYNIQRKIVLMA
                     IWVAIPAALWFAPSLGGLIQPHVDMRGFVHDVGIAWLSLGAAGLVFRVIQLCVTRNVT
                     EGLAWGFKIITDPFMDIVLYHKAPLKLMQGQMISSVASPTMPTAAH"
     gene            3547..4710
                     /locus_tag="VIN75_15330"
     CDS             3547..4710
                     /locus_tag="VIN75_15330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8975589.1"
                     /translation="MTETYPPEINGVAMTVARLVQGLRARHHSVRVIRPRQASDAAAT
                     PIDGDVLVRGVPIPRYEGLKMGLPCTGRLVRLWRRERPDVVHIATEGPLGRSALLAAR
                     ALGLPVCSEFRTNFHAYSAHYGLGFMKTLILGYLRRFHNATQCTMVPTQALHDQLQRE
                     GFRDLLTVARGVDTRRFDPARRSEGLRAQWGAAPDDLVVTCVGRLAPEKNLATLLDAF
                     DAIHRAQPRARLVLVGDGPMRKELEARRPDAIFAGQRMGDDLAAHYASADLFLFPSIT
                     ETFGNVTTEAMASGLAVVAYDYAAAQRVIRDGENGALAPYDDRGAFVATAARTAADLA
                     RCRVLGGRARTSVMALDWDGIAAQVEGVMASVIARKAERPAPYAFASARHSSV"
     gene            complement(4674..5918)
                     /gene="lplT"
                     /locus_tag="VIN75_15335"
     CDS             complement(4674..5918)
                     /gene="lplT"
                     /locus_tag="VIN75_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY8975590.1"
                     /translation="MPASFLLLLAAQFTSALADNALLIVTIDLLQQRAMPGWLAPMLK
                     VAFIAAYVLLAPFVGPLADAFPKARLMAWMNGVKIAGVTALVAGLHPVAAFLVVGLGA
                     AAYAPAKYGLATELVRPDQLVAANAWIETAAVCAVLLGTGLGGLLVSPWLLGHASRIV
                     SDVCDPVLPSLLILLATYLLAGALNLGVPDSGVRHEVGSLHPRAMLRDFARANRRLWR
                     DRDGGLSLTVTTIFWGVGATLQFAVLRWARETMHLTLDRAAFVQAAVAIGVIAGAVAA
                     GRFVPLAHAKRMLWAGVLFGLLMTVLPFLTQLRLAVAVLIVVGGVGGLMVVPLNALLQ
                     DRGNALLSAGRSIAVQGGNENASVLVCLGVYSALCAVDVSLAALMTGLGACVSAALAW
                     LLLQERWRAPLHTDEWRAEAKA"
     gene            complement(6194..8164)
                     /locus_tag="VIN75_15340"
     CDS             complement(6194..8164)
                     /locus_tag="VIN75_15340"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY8975591.1"
                     /translation="MTATAYERHLERAHALLSRRVRSGAGGLATDIAAAPVTNAALRE
                     LRGEVATALARAHAAFATAFSEALRESFRQATQAPAAKPAGQWLDLGAFEIMDESRIQ
                     DEIEIAQVVRLLEEDCAVELRRLIKFEGALRRSEGVRVATSPIAPPSLARCLWSASEV
                     LGLAPAARAEAVRCVARWLAPRLAEIYAAVRQAGLADASQIEADRVAEPDDDTSTASP
                     PTGFDVTRPGALYELMDLDEERPVFRTSTQAAPLTIIPDTLPMPLEGDGARIPGLIHS
                     HREELAALQDATSTRLGGALIGHLFEQIVADPALSADGRGWIARLEPSVVQLAASDAS
                     LLKSHRHAAWVLVNRVATQMQDDKGAPAPEFMQWLQESVAQIEMQPATATFEAAVERL
                     AQWQRRQAQKRLTSVEGALELLQRNATLEDLVAEAKARLERKLDSAHPPSPTRRFIRT
                     LWALVVAQEWTTLPPEARRNSPAMDTAVDVIWSTDKARSRADAPTLVAMVPELVERVT
                     AGMATVKLSDAMRKAWLDQIAAIHLHVMRRPEGEEAVAGPVTVDLQLDDELPPPPPPP
                     APPPVDASSPVASLQVGDSVTLQLHGEWTDLQLLWVSDNGYFLLFAGAGDATQSFTRK
                     ALEKMAAQGLLRTADASSALQRASDELIRRRL"
BASE COUNT         1241 a         2943 c         2900 g         1146 t
ORIGIN      
        1 gcgctggcct gcgagcaggc gcccaacctg cgccgctacg cgccgctgcc gctctgaagc
       61 cccccgcgcc acgcgcccgc gggcgcgagc gggtcacgcc tgcgcggtcg cgagcgcctc
      121 gcgcgacccg gacgcgtcca cgccggccgc gaccgccggc aggcgcatca gcgcctggcc
      181 catgcggatg cgcgcgccag tggcgatgcc gtcgacgaac tcgaagcccg gcggcacgaa
      241 catgatgatg gtcgagccgt gctggaagcc gcccatctcc tcgcccttgc cgaagcgcgc
      301 ctcgaccggg atctcgttgg gcccgcggta cttcaggtgc agcagcacgt ccgtgaactt
      361 caggcggatg ctcgcgacga ggatcgccgc caccggcacg atcgcgatcg gatggttgcc
      421 gcggcgcagc cgcgcgctga gcaccgcgcg ttcgttgcgg cagaacaggc gctcgacgcg
      481 cctgagcgcg atcgggttga cgttccaggt gtcgccggtg atgtagctga cgtggttcac
      541 gtcgacgtcg tgcggcgcat ggaagcggtg gtacatcgcc gacgtcagcc gcagcgtgat
      601 gaagcggccg tcgcggaacg ggctcgtgtc ggtgccgggc ccgaacaggt cttccatccg
      661 gtacgggaag cccttggcct ggaacaccgt cgtgcccgag accggcccgc aggcgcccac
      721 gatggcgtcg cacggcgagg tgagcacgcc cggatcgccg tcgaccggac gcgcgccggg
      781 catcagctcg cgcgtgaagc agtcgtgcat cgagcggaac ttgcgcgtgc gggcgtcgct
      841 caggtcgagg tcggtgaaca acttccacac gccgatcgag gccttgctga cgatcgggtt
      901 gcggatcttg ctgaacttgc ccatgaagtg cgtcagcgcg atgcgcggca cgcggttggt
      961 gagcaggaag ttcaggtctt cctgcgcgag cagcttgcgg aattgctttt tcatcggccc
     1021 tcagctgctg aagcgttcac gcagcatttc gcgcagcagc ccgcgccgga tggtgcggtt
     1081 gctctgcgcg ggatcgttcc accaccagcc gtcgccctgc atctggacgg ccgcggcgac
     1141 gaagcggtct atcacggcgt cgaagtcggc atcgccgtag tcgaggctga agatcaggcg
     1201 ccccgtgccc acccagctga gcgccaggcc gtgcgcgcgc aggtagaact gcagcatcca
     1261 gttgtagcgc gacggctgct cgtacagcac cgtccagatc gactgcaggt tggccacgcg
     1321 caccggcacg cccgccgcgg ccagccgctc gttgaagcgc gcggcgcgct ggttccagcg
     1381 ctcgtcgacg ccgtcgtaca tggcccggac gtcggggcgc gccaggcgct cgaggaacac
     1441 gttcatcgcc cccatcacgt acgggtgcga gttgaaggtg ccgcgggcaa agcagatgtc
     1501 ggcgggccgg tcctcgcgga agcgcttcat caggtcgcgc cggccgcaca ccacgcccac
     1561 gggcagcccg ccacccagcg acttgccgta ggtgaccatg tcggcctgga tgccgaaata
     1621 ctcctgcgcg ccgccgggcg cgaggcgaaa gccgatgaag atctcgtcga agatcagcac
     1681 gatgccgcgc tcggtgcaga cctggcgcag ctgcctgagc cactcggtgt acgccgcgcg
     1741 gtcgaacttc gcgcggcgcg cgctgtcgac cagcgacgag tcgcccggcg ccgccgcgtt
     1801 cggatgcagc gcctgcaacg ggttgaccag cacgcaggcg atgtcctggc gcgtgcgcag
     1861 caccttcagc gtcccggctt tcatctcctc gagcgtgtac gtctcgcgcg gcggcagcgg
     1921 gttgccgggg cccggctgca cgtcctccca ccagccgtgg tacgcgccgc agaagcgcac
     1981 gaggaatttc ttcttcgtgt gatagcgcgc caggcgcacc gcctgcatca cggcctcggt
     2041 gccggacatg tggaacgaca cctcgtccag acccgagatg cgctgcaggt ggcgcacgtt
     2101 gtccagcagc accgggtggt aggcgcccag caccgggccg agatcggcga cgcgtgcggc
     2161 gccttcggcg atgcattcct tgtagaagtc atagccgaac aggttgacgc cgtacgagcc
     2221 ggtgaggtcg tggaaggtgt tgccgtccag gtccgtcacg gtcacgcccg acgatgactg
     2281 caggaagctg cccgtcttca ggtgctggcg caggtacggg ctgtactgga acggcacgcg
     2341 atagctgccg gtgaactgca ggtcggagat gccgctgctg gcctgggcgg tcagcgcggc
     2401 gctcctggcg aagcgctccg tgtacagcgc gacgagcccg gcgaagccgg cgcggcgccg
     2461 ctgctggaac tcctgcggcg cgccgtcgga gccgaagaag cgcgcctcgt cgtaggcgta
     2521 gcccggcagg aagccggcca cgcgcttggc catcttcgaa tggcccgtga gcgagcgatg
     2581 cttggcgcgc gagagttcga gccgccgctt gatgcgcggg gccagcacgg ccagcgcggc
     2641 gagcagcagt gcgcagaagg cgagggtgga cggttccatg ggcgacggga tcccgcgata
     2701 tctcagtgag ccgccgtggg catcgtcggc gaggcgacgg acgagatcat ctggccctgc
     2761 atcagcttga gcggcgcctt gtggtacagg acgatgtcca tgaacgggtc ggtgatgatc
     2821 ttgaagcccc aggccaggcc ttccgtcacg ttgcgcgtga cgcacagctg gatcacgcgg
     2881 aacacgaggc cggccgcgcc gagcgacagc cacgcgatgc ccacgtcgtg aacgaagccg
     2941 cgcatgtcga catgcggctg gatcagtccg cccagcgagg gtgcgaacca cagcgctgcg
     3001 ggaatcgcca cccagatcgc catcagcacg atcttgcgct ggatgttgta gccggccttg
     3061 acctcttcct tgtactcgtc cgagacctgg ttgacgtggt cgtagccgcg cggctcgaag
     3121 aagaagtggc cggcctggcg cgtggtcatc gacaccgtcc aggccagcac ggcggcgatc
     3181 gccggatcga tcagcagcag gccgtacgag atgcagaaca cgatcgcgct gaccaggtgc
     3241 aggctctggt tgatcgtgct gtggtggtag aagcgatggt cgtcccaccg cagcgtgcgc
     3301 agcgtttgca tgaaggtgtt cacgagggtt cctctcagag attcgccgct gcacgacaag
     3361 accgcggcgt gacaggatgg tgacgtcgaa atatgaaagg gccgtgtccg ctggtggggt
     3421 gaattgccgc ggaactgtca tcgatctgtt gcacgcgccg gccatcatcg ccagcgaccg
     3481 caacgaatcg gaggttcact cgtggacgtt cagaccggcc ttcaacggcc cctgcgaatt
     3541 gcctgcgtga ccgagaccta cccgcccgag atcaacggcg tcgcgatgac cgtggcccgc
     3601 ctcgtgcagg gcctgcgcgc gcgccaccac agcgtgcgcg tgatccgccc gcgccaggcg
     3661 agcgacgctg cggcgacgcc gatcgatggc gacgtcctcg tccgcggcgt gcccatcccg
     3721 cgctacgaag gcctgaagat gggcctgccg tgcaccggcc ggctggtcag gctgtggcgc
     3781 agggaacggc cggacgtcgt gcacatcgcc acggaaggtc ccctcggccg ctccgcgctg
     3841 ctggccgcca gggcgctggg cctgccggtg tgctccgagt tccgcaccaa cttccacgcc
     3901 tacagcgcgc actacggcct gggcttcatg aagacgctca tcctgggcta cctgcgccgc
     3961 ttccacaacg ccacgcagtg caccatggtg cccacgcagg cgctgcacga ccagctgcag
     4021 cgcgagggct tccgcgacct gttgaccgtc gcgcgcggcg tcgacacgcg ccgcttcgac
     4081 cccgcccgcc gcagcgaggg cctgcgcgcg caatggggcg ccgcgcccga cgacctggtc
     4141 gtgacctgcg tgggccgcct cgcgcccgag aagaacctgg cgacgttgct ggacgccttc
     4201 gacgcgatcc accgcgcgca gccgcgcgcc cgcctcgtgc tggtgggcga cggcccgatg
     4261 cgcaaggaac tcgaggcgcg ccggcccgac gcgatcttcg caggccagcg catgggcgac
     4321 gacctcgccg cccactacgc atcggccgac ctgttcctgt tccccagcat caccgagacc
     4381 ttcggcaacg tcaccaccga ggcgatggcc agcggcctgg ccgtcgtcgc gtacgactac
     4441 gccgccgcgc agcgcgtgat ccgcgacggc gagaacggcg cgctggcgcc gtacgacgac
     4501 cgcggcgcgt tcgtcgccac cgccgcgcgc accgccgccg atctcgcgcg ctgccgcgtc
     4561 ctcggcggcc gcgcccgcac cagcgtgatg gcgcttgact gggacggcat cgccgcgcag
     4621 gtcgaaggcg tcatggcgtc ggtgatcgcg cgcaaggccg aacgccccgc gccctacgcc
     4681 ttcgcctctg cgcgccattc gtcggtgtga agcggggcgc gccagcgctc ctgcagcagc
     4741 aaccaggcca gcgcggcgga aacgcacgcg cccaggcccg tcatcagcgc ggcgagcgag
     4801 acgtccaccg cgcacaaggc cgagtacacg cccaggcaca cgagcacgct ggcgttctcg
     4861 ttgccgccct gcaccgcgat cgaacgtccc gcgctcagca aggcgttgcc gcgatcctgc
     4921 agcaacgcgt tcagcggcac caccatcagg ccgcccacgc cgccgacgac gatcagcacc
     4981 gccacggcca ggcgcagctg cgtcaggaac ggtagcaccg tcatcagcaa cccgaacagc
     5041 acgccggccc acagcatgcg cttggcgtgc gccagcggca cgaaacggcc ggcggcgacg
     5101 gcgcccgcga tgacgccgat cgcgacggcg gcctgcacga acgcggcgcg gtccagcgtc
     5161 aggtgcatgg tctcgcgcgc ccagcgcagc accgcgaact gcagcgtcgc gccgacgccc
     5221 cagaagatcg tcgtcaccgt cagcgacagt ccgccatcgc ggtcgcgcca cagcctgcgg
     5281 ttggcccggg cgaagtcgcg cagcatcgcg cgcgggtgca gcgagccgac ctcatgccgc
     5341 acgccgctgt cgggcacgcc gaggttgagc gcgccggcca gcaggtaggt ggcgagcagg
     5401 atcagcagcg atggcagcac cggatcgcag acgtcggaca cgatgcggct cgcatgcccc
     5461 agcagccacg ggctgacgag cagtcctccg agcccggtgc ccagcagcac ggcgcacacg
     5521 gcggccgtct cgatccatgc attggccgcg acgagctggt cgggccgcac gagctcggtc
     5581 gccaggccgt acttggccgg cgcgtacgcc gcggcgccca ggccgacgac caggaacgcc
     5641 gcgaccggat gcaggcccgc cacgagcgcc gtgacgccgg cgatcttgac gccgttcatc
     5701 cacgccatca gccgcgcctt cggaaacgcg tcggccagcg gcccgacgaa cggcgccagc
     5761 agcacgtatg cggcgatgaa cgcgaccttg agcatcggcg ccagccagcc cggcatcgcg
     5821 cgttgctgca gcaggtcgat ggtgacgatc agcagcgcgt tgtccgccag cgccgaggtg
     5881 aactgggccg ccagcagcaa caggaacgag gcgggcatca ggcggcgcgg tcgaaggaaa
     5941 cgggcatcgc tggcgtcgag tcaggatgtg gacagcacgc gagcctgagg cacgtgcgtg
     6001 aaacggtcgt gacaagcccg cgacagcatg aactccgcgc cacgcagggc gggttagccg
     6061 caggcgtgac ccgccgttcg cctgcgagct gcgatgggac tttcgtattg aaggcgctgg
     6121 ctggatcggc gcgccaccga atggcgggct acgccttcgg ccaacccgcc ctacgtagac
     6181 gcgccttgcg cgtctacagc ctgcgccgga tcagttcgtc gctcgcgcgc tgcagcgcgg
     6241 aactggcgtc ggcggtgcgc agcaggccct gcgcggccat cttctcgagc gccttgcgcg
     6301 tgaagctctg cgtggcgtcg ccggcgcccg cgaacagcag gaagtagccg ttgtcgctca
     6361 cccacagcag ctgcaggtcg gtccactcgc catgcagctg cagcgtgacg ctgtcgccga
     6421 cctgcagcga cgcgacgggc gacgacgcgt ccaccggcgg cggcgccggc ggcgggggcg
     6481 gcggcggcag ctcgtcgtcg agctgcaggt cgaccgtgac ggggccggcg acggcctcct
     6541 cgccctcggg ccggcgcatc acgtgcaggt ggatcgcggc gatctggtcg agccaggcct
     6601 tccgcatcgc atcggacagc ttgaccgtgg ccatgcccgc ggtcacgcgc tcgaccagct
     6661 cgggcaccat cgccaccagc gtcggcgcgt cggcgcgcga gcgcgccttg tcggtggacc
     6721 agatgacgtc gaccgcggtg tccatcgccg gcgagttgcg ccgcgcctcg ggcggcagcg
     6781 tcgtccactc ctgcgcgacg accagcgccc acagcgtgcg gatgaagcgc cgcgtgggcg
     6841 acggcggatg cgccgaatcg agcttgcgct ccagccgcgc cttggcctcg gccaccaggt
     6901 cctccagcgt ggcgttgcgc tgcagcagct ccagcgcgcc ctcgaccgag gtgagccgct
     6961 tctgggcctg cctgcgctgc cactgcgcca ggcgttccac cgcggcctcg aacgtggccg
     7021 tggccggctg catctcgatc tgcgccaccg actcctgcag ccactgcatg aactcgggcg
     7081 cgggggcgcc cttgtcgtcc tgcatctgcg tggcgacgcg gttgaccagc acccaggccg
     7141 cgtgccggtg cgacttcagc agcgaggcat cgctcgccgc cagttgcacg accgacggct
     7201 ccaggcgcgc gatccagccg cggccgtcgg ccgacagcgc cgggtcggcc acgatctgct
     7261 cgaacaggtg gccgatcagc gcgccgccca ggcgcgtgct ggtggcgtcc tgcagcgcgg
     7321 cgagctcctc gcgatggctg tggatcagcc cggggatgcg ggcgccgtcg ccctccagcg
     7381 gcatcggcag cgtgtcgggg atgatcgtca gcggcgccgc ctgcgtcgag gtgcggaaga
     7441 cgggccgctc ctcgtcgagg tccatcagct cgtacagcgc ccccggccgc gtgacgtcga
     7501 agccggtggg cggcgatgcg gtggacgtgt cgtcgtcggg ctcggcgacg cggtcggcct
     7561 cgatctgcga cgcgtcggcc aagcctgcct ggcgcacggc ggcatagatc tcggccagcc
     7621 gcggagccag ccagcgcgcg acgcagcgca ccgcttcggc gcgcgccgcc ggggcgagcc
     7681 cgagcacctc ggaggccgac cacaagcacc gcgcgaggct cggcggcgcg atgggcgagg
     7741 tcgcgacgcg cacaccctcg ctgcggcgca gggcgccctc gaacttgatg agccggcgca
     7801 gttcgaccgc gcagtcctcc tcgagcagcc gcaccacctg ggcgatctcg atctcgtcct
     7861 ggatgcgcga ctcgtccatg atctcgaacg cgcccaggtc cagccactgt ccggccggct
     7921 tggccgcggg cgcctgcgtg gcttgcctga acgactcgcg cagcgcctcg ctgaacgcgg
     7981 tcgcgaacgc cgcatgcgcg cgcgccagtg ccgtggcgac ctcgccgcgc agctcgcgca
     8041 gcgcggcgtt ggtcaccggc gcggcggcga tgtcggtggc gagcccgccc gcgccgctgc
     8101 gcacgcggcg cgacagcaac gcatgggcgc gctcgaggtg gcgttcgtag gcggtggcgg
     8161 tcacgcggcc atattaggcc aggaccgccg tccgcaccgt cggcggcgtg ttgcccgacc
     8221 gtggttgtgc
//
LOCUS       DATCRO010000560         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_839038, whole genome shotgun sequence.
ACCESSION   DATCRO010000560 DATCRO010000000
VERSION     DATCRO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839038"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VIN75_15345"
     CDS             complement(<1..175)
                     /locus_tag="VIN75_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8975592.1"
                     /translation="MEFFRIRRDIPFMRHALVFNIISALTFLAAVFFLVTRGLNFSIE
                     FTGGTAVEATYAQS"
     gene            complement(203..2131)
                     /gene="secD"
                     /locus_tag="VIN75_15350"
     CDS             complement(203..2131)
                     /gene="secD"
                     /locus_tag="VIN75_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8975593.1"
                     /translation="MNRYPVWKFVLMALAVLIGALYTVPNLYGQAPAVQVSAAKLTTK
                     VGPDTATQVQALLDAASIKADYVTFEGTSVHARFANPDIQIHARDVINAGLNAGADKD
                     SPPYSVSLALVARAPRWMTALHAMPMYLGLDLRGGVHFLLQVDLKAALEKKTEGLVTD
                     TRTLLRQKQLRTVVTRDGNAVTMTFHDAATATQAVNVLTDSFPDVQWNQVAEGTDVKI
                     SGALKPAALVKAQEAAIKQNMTTLANRVAGLGTSEPVIQQQGPDRIVVELPGIQDTAK
                     AQELLGRTATLEFHMVDESAQGNAAAEGTGPVPFGDDRYENSPDVGRRHGGYTVVKKD
                     VIASGDDLTDAQANFDQQNQGSIVILNLNAKGALNMREVSGQNIGKLLAIVLFEKGKG
                     QAISVATIQSELGDRFNITGMPTAEQANDLALLLRSGALAAPMEIIEQTAIGPSLGAD
                     NIAKGLHSVAWGFVAILVFMCAYYLLFGVISTLSLGVNLLLLVAVLSLIQTTLTLPGI
                     AAIALTLGMAIDANVLVNERIREELRGGSAPQTAISAGYERAWATILDSNITTLIAGL
                     SLLAFGSGPVRGFAIVHCLGILTSMFSAVFFSRGLVNLWYGRQKKLKSVLIGQVWKGG
                     AKAGASDEAAANTPGAAR"
     gene            complement(2220..2546)
                     /gene="yajC"
                     /locus_tag="VIN75_15355"
     CDS             complement(2220..2546)
                     /gene="yajC"
                     /locus_tag="VIN75_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY8975594.1"
                     /translation="MFLISSAFAQTAAPAASGASDNSFLFMLIPMFAIMYFLVIRPQQ
                     KRAKEQKDLIAALVKGDEIVTTGGIIGRISKLGESYVHIETGGNVELQVQRSAIVQVL
                     PKGTLK"
     gene            complement(2742..3470)
                     /locus_tag="VIN75_15360"
     CDS             complement(2742..3470)
                     /locus_tag="VIN75_15360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8975595.1"
                     /translation="MPERPATPGAERRAAGVAPVDPLAAALAACADLVPRLAAVQSLG
                     DPAEQRQALLQLPATLQAASAALARLAASADAAVSHDTRGDALTGLADRAQLEAACAG
                     LALQFPEAPLTLVAVDVDHLRHVNDHRSRATGDAVLRAVAEVLRAQSRPQDVLARADG
                     GLFVVVLGGPVSTTRALLVAERLRAAIEGHAWAALGDGLRVTASLGVAARTPGEPLED
                     VLARAGAALQECKRGGRNQVRSRA"
     gene            3603..4505
                     /locus_tag="VIN75_15365"
     CDS             3603..4505
                     /locus_tag="VIN75_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY8975596.1"
                     /translation="MSLVILLAAAVLLVGAIAGLPRWRAWRRQLWASRPFPAAWRNVL
                     RRRVPLYRQLPADLQLQLRRRILEFLAEKPFLGCAGLVVTDEMRVTIAAQACLLRLNH
                     RGPLFPELRQVLVYPGAFVVDRVHAAPGGVLREERRALAGESWQQGQVILSWQDVLDG
                     ARIPDDGHNVVLHEFAHQLDQETGAANGAPLLGSRAAHARWAGVMTREFAGLQARIRA
                     RDAARAQAGRSWWDGSPEPVPAPPPDDLISDYGATNEAEFFAVVTEVFYEQPRALAAR
                     HPELFALLRDYYRVDPREWLAPMR"
BASE COUNT          618 a         1581 c         1689 g          681 t
ORIGIN      
        1 cgctctgcgc gtacgtggcc tcgacggccg tgccgccggt gaactcgatc gagaagttca
       61 ggccgcgggt gaccaggaag aacacggccg cgaggaacgt gagcgccgag atgatgttga
      121 acaccagcgc gtgccgcatg aacgggatgt cgcgccggat acggaagaat tccatgagcg
      181 tttccttcgg atctgggacg gttcagcgcg cggcgccggg ggtgttggcg gcagcctcgt
      241 cggaggcgcc ggccttggcg ccgcccttcc agacctggcc gatcagcacc gacttgagct
      301 tcttctggcg gccgtaccag aggttgacga ggccgcgcga gaagaacacc gccgagaaca
      361 tcgacgtcag gatgcccagg cagtgcacga tggcgaaacc gcgcacgggc cccgagccga
      421 acgccagcag cgacaggccg gcgatcagcg tcgtgatgtt ggagtcgagg atggtggccc
      481 aggcgcgttc gtagccggcc gagatcgccg tctgcggcgc gctgccgccg cgcaactcct
      541 cgcgtatgcg ctcgttgacc agcacgttcg cgtcgatggc catgcccagt gtcagcgcga
      601 tggccgcgat gccgggcagc gtcagcgtcg tctggatcag cgacagcacc gccaccagca
      661 gcagcaggtt cacgcccagc gacagcgtcg agatcacgcc gaacagcagg tagtacgcgc
      721 acatgaacac caggatcgcc acgaagcccc aggccacgct gtgcaggccc ttggcgatgt
      781 tgtcggcgcc caggctgggg ccgatggcgg tctgctcgat gatctccatc ggcgcggcca
      841 gcgcgcccga gcgcagcagc agggccaggt cgttggcctg ctcggcggtc ggcatgccgg
      901 tgatgttgaa acggtcgccc agctcgctct ggatggtggc cacgctgatc gcctggccct
      961 tgcccttctc gaacagcacg atggccagca gcttgccgat gttctggccc gagacctcgc
     1021 gcatgttcag cgcgcccttg gcgttcaggt tcaggatgac gatcgagccc tggttctgct
     1081 ggtcgaagtt ggcctgcgcg tcggtcaggt cgtcgccgct ggcgatgacg tccttcttga
     1141 cgacggtgta gccgccgtgg cggcggccga cgtcgggcga gttctcgtag cggtcgtcgc
     1201 cgaacggcac cgggcccgtg ccctcggccg cggcattgcc ctgggccgac tcgtcgacca
     1261 tgtggaattc cagcgtcgcg gtgcggccga ggagttcctg ggccttggcg gtgtcctgga
     1321 tgccgggcag ctcgacgacg atgcggtcgg ggccctgctg ctggatgacg ggctcggagg
     1381 tgcccaggcc ggcgacgcgg ttggccagcg tcgtcatgtt ctgcttgatg gccgcttcct
     1441 gcgccttgac cagcgcggcg ggcttgagcg cgcccgagat cttgacgtcc gtgccctcgg
     1501 cgacctggtt ccactgcacg tccgggaacg agtcggtcag cacgttgacg gcctgggtgg
     1561 cggtggccgc gtcgtggaag gtcatcgtga cggcgttgcc gtcgcgcgtg acgaccgtgc
     1621 gcagctgctt ctggcgcagc agggtgcggg tgtcggtgac caggccttcc gtcttcttct
     1681 cgagcgcggc cttcaggtcg acctgcagca ggaagtgcac gccgccgcgc aggtccaggc
     1741 ccaggtacat cggcatcgcg tgcagcgccg tcatccagcg cggcgcgcgc gcgacgagcg
     1801 ccagcgacac ggagtacggc ggcgaatcct tgtcggcgcc ggcgttcagg ccggcgttga
     1861 tcacgtcgcg ggcatggatc tggatgtcgg ggttggcgaa gcgcgcatgc accgacgtgc
     1921 cttcgaaggt gacgtagtcc gccttgatgg atgccgcgtc gagcagcgcc tgcacctgcg
     1981 tggccgtgtc ggggccgacc ttggtggtca gcttggccgc cgagacctgc acggccgggg
     2041 cctggccata caggttgggc acggtgtaga gcgcgccgat cagcacggcg agcgccatca
     2101 gcacgaattt ccagacgggg tagcgattca tacgtgtttc ctcgaaggcg tcgcggcgcg
     2161 cactcgggcg cgcgcgggcg acggcaccgg ccgcgacgcg cgcggccggc gggcactgct
     2221 tacttcaacg tgcccttggg caggacctgg acgatggcgc tgcgctggac ctgcagctcg
     2281 acgttgccgc cggtctcgat gtggacgtac gactcgccca gcttggagat gcggccgatg
     2341 atgccgccgg tcgtgacgat ctcgtcgccc ttgaccagcg cggcgatcag gtccttctgt
     2401 tccttggcgc gcttctgctg cgggcggatc acgaggaaat acatgatcgc gaacatcggg
     2461 atcagcatga acaggaagct gttgtcggac gcgccggagg ctgcgggcgc cgcggtctgg
     2521 gcgaaggcgg aagagatcag gaacacggcg aaatcctcag ggaaggcttg ggaagcgggg
     2581 ccgtcgcggc agggggccgc ggccccgctc tcgaaacgtg cggtcgaggt ggagccgcca
     2641 cgaggcgatt caaccccggg atagcccgac caacccacca ttttaacggg cgcgggcgga
     2701 cgccccgccc ggccccgggc gcaggcttgc ccgcggggca ttcaggcgcg cgagcgcacc
     2761 tggttgcggc cgccgcgctt gcactcctgc agcgcggcgc cggcgcgtgc cagcacgtcc
     2821 tccagcggct cgcccggcgt ccgcgccgcg acgcccagac tggccgtcac ccgcaggccg
     2881 tcgcccagcg ccgcccaggc gtggccctcg atggcggcgc gcaggcgctc ggccaccagc
     2941 agggcgcgcg tggtcgacac cggcccgccc agcacgacca cgaacaggcc gccgtcggcg
     3001 cgcgcgagca cgtcctgcgg ccgcgactgc gcccgcagca cctcggcgac cgcgcgcagg
     3061 acggcgtcgc cggtcgcgcg cgagcgatgg tcgttgacgt ggcgcaggtg gtcgacgtcg
     3121 accgccacga gggtcagcgg ggcctcgggg aactgcagcg ccaggcccgc gcaggcggcc
     3181 tccagctggg cgcggtcggc caggccggtg agcgcgtcgc cgcgggtgtc gtgcgagacg
     3241 gcggcgtccg cgctggcggc caggcgcgcg agcgcggcgc tggccgcctg cagcgtggcg
     3301 ggcaattgca gcagggcctg gcgctgctcg gccggatcgc ccagcgactg cacggccgcc
     3361 agccgcggca ccaggtcggc gcaagcggcc agcgccgcgg ccagcggatc gacgggcgcg
     3421 acgcccgccg cccggcgctc ggcccccggc gtggccgggc gttcgggcaa ggggagcgcg
     3481 tgcgggtcca aggtctgcat ccggttggcg acgcctgatc gtaagtccgg cggacgactt
     3541 gaaactgtac ggccgatggc catatgctgg agatcgcgcc gcggcgcccc tcgagcaacg
     3601 ccatgtccct cgtcatcctg ctggccgcgg ccgtgttgct cgtcggcgcc atcgccggac
     3661 tgccgcgctg gcgcgcctgg cgccgccagc tgtgggccag ccggccgttc ccggcggcgt
     3721 ggcgcaacgt cctgcggcgc cgcgtcccct tgtatcggca gttgccggcc gacctgcagc
     3781 ttcaattgcg ccgccgcatc ctcgagttcc tggccgagaa gccgttcctc ggctgcgcgg
     3841 ggctggtcgt caccgacgag atgcgcgtca cgatcgccgc ccaggcctgc ctgctgcgcc
     3901 tgaaccaccg cggcccgctg tttcccgagc tgcgccaggt gctcgtctat ccgggcgcgt
     3961 tcgtcgtcga ccgcgtccac gcggcgcccg gcggcgtgct gcgcgaggaa cggcgcgcac
     4021 tggcgggaga gtcgtggcag caggggcagg tgatcctgtc ctggcaggac gtgctggacg
     4081 gcgcccgcat ccccgacgac ggccacaacg tggtgctgca cgagttcgcc caccagctcg
     4141 accaggagac cggcgccgcc aacggcgcgc cgctgctggg ctcgcgcgcc gcgcacgcgc
     4201 gctgggccgg cgtgatgacc cgcgagttcg ccggcctgca ggcgcgcatc cgcgcgcgcg
     4261 acgcggcgcg ggcgcaggcc gggcggtcgt ggtgggacgg gtcgcccgag cccgtgcccg
     4321 cgccgccgcc cgatgacctg atcagcgact acggcgccac caacgaggcc gagttcttcg
     4381 ccgtcgtcac cgaggtgttc tacgagcagc cccgggcgct cgccgcccgc catccggagc
     4441 tgttcgcgct gctgcgcgac tattaccggg tcgatccgcg ggagtggctg gcgccgatgc
     4501 gctgagggcg gcgggcgaat ggcgggttac gcctgcggct aacccgccct tgtatcttta
     4561 cgtccgtgg
//
LOCUS       DATCRO010000561        10790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_51308, whole genome shotgun sequence.
ACCESSION   DATCRO010000561 DATCRO010000000
VERSION     DATCRO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51308"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VIN75_15370"
     CDS             <1..1456
                     /locus_tag="VIN75_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975597.1"
                     /translation="RIEATRSRDLQRRVDRMQAIRAELDPGVAPLLPGAGDGSPDALA
                     ARAQALADAIDAADREQRARDLARLTQIPLALARRKGDADDAAHRLPPPPETAQDRIA
                     RLERHARDLAEARRARLDAQREGLRVTRAHVKAHRIHEPDAALAHTAPASGSTGQAHV
                     ADGIPRSMTEQFKSMVRLGGPQGRVGVVGTQAGLSVNREHVKGIAGGRNAKAEDFGRD
                     ADVASKGRQVVLEGQREKAGQGGGIGDHGSGSSRHGSAASTRGDASLRGLAAVRIPGS
                     SLDLTGRVEDAQHTFVRYASPPAVDLATLRTAAGRVFGPGGTHAERVYLDTWYVIGPF
                     AGKGAESQQAGYPPEDDVDLDAAYPGPEGRTLTWQFTSRGFYPFVPPDRRMDAVYYAY
                     TEIRVDAERDVWFSLAADDDSMMWLDERLVWISSPGDKPWYHPPYYARDELVGSLSLT
                     EGRRRVHLAAGVHRLLFKLCNADGHPFFSVVLSK"
     gene            1465..2097
                     /locus_tag="VIN75_15375"
     CDS             1465..2097
                     /locus_tag="VIN75_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8975598.1"
                     /translation="MQLNLFQQLATGGAGIWVIAALSLLMVAVAIERFHRFRMSAIVP
                     TGLVDEVLPLWQAGRLEELQQALARRESVLAMAIRFVIANRARWTPEVLDERAGELAS
                     RALRQQQQRAYPLAVVATVAPIVGLLGTVVGMIEAFHVIAFTGGMGDASLLAGGISKA
                     LVNTAAGLSVALPALALHHFFRHRLTLGGLALEEQLERVMDACARDGGRA"
     gene            2094..2522
                     /locus_tag="VIN75_15380"
     CDS             2094..2522
                     /locus_tag="VIN75_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8975599.1"
                     /translation="MRIAVGEDEQPEISLIALIDCIFFLLMFFMVATSFQKQAEGKAA
                     RQLPITLPQSEVSLDEAQADASPLVIGVDVKGGLYWGREPVSTVQLRDRLHAEARKDP
                     NRAVRIDGDTLVPYQDVVHLLDLCEFEGLTRVALRTRAGR"
     gene            2707..3093
                     /locus_tag="VIN75_15385"
     CDS             2707..3093
                     /locus_tag="VIN75_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY8975600.1"
                     /translation="MTDTELADLYRDYIACLNRRDWPALAQFVSHDVMHNGRPLGLSG
                     YRAMLEKDVEDVPDLKFNVEMVTCDPPNVAAKLAFDCTPTGQFQGLAVNGKQVRFTET
                     VFYEISDRRIATVWSAIDKAAIEAQL"
     gene            complement(3147..3902)
                     /locus_tag="VIN75_15390"
     CDS             complement(3147..3902)
                     /locus_tag="VIN75_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HEY8975601.1"
                     /translation="MNEPAKDALPPHAVARLRDACLADDAQRPRLDRGAPRKARCERC
                     RLAPSHCACALRPRVPTRAGMCLLMAPFEALKPSNTGWLVADVVPDTHAFAWSRTHVD
                     PALVALLDDPRWQPFIVFPGEFAAPERVVGALPAVAPGDARRPLFILLDGTWAEARKA
                     FRKSPYLDRFPVLGLDPATLSRYRLRSAWHEHHQCTAEIAALCLDLSGDARAARTLEA
                     WLDVFSERYLCAKQSVPPDESDEAHRRLAALRG"
     gene            complement(3937..4338)
                     /locus_tag="VIN75_15395"
     CDS             complement(3937..4338)
                     /locus_tag="VIN75_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8975602.1"
                     /translation="MTAPVLETWHRLVRTNDASGIPALIAEDATFHSPVVHAAQPGRK
                     LTVMYLSAAFRVFFNPTFRYVREIAGPHDAMLEFETEIDGILVNGVDIIKWNDAGQIV
                     DFKVMIRPLKGINIIHQKMGEMLAAAQKPAA"
     gene            complement(4420..6216)
                     /locus_tag="VIN75_15400"
     CDS             complement(4420..6216)
                     /locus_tag="VIN75_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8975603.1"
                     /translation="MNTAAPPTRLSLSTVVSLALALVLAACSTVDLAPAPAAGPAPVV
                     TAAMQPADAASASEAAASQPAVALAAAGSASAPAAAASDAEAMEADDDDDSDAAKTGT
                     APAVLPPYDPLQPDTRVNLDDSAARSDLWHRVRDGFAIPDMDTPAVKQWEQWYSSRPD
                     YVARMTARGARYLYHITQEVERRHMPTELALLPFTESAFNPQAMSVAKASGMWQFVSA
                     TGKDFDLKQNIFRDDRRDVLSSTRAALDYLQRLHDMFGDWQLALAAYNCGQGNVQRAI
                     ARNQKRGLPTDYASLNLPKETSEYVPKLQAVKNIVLHPEAYSLTLPPVGNHPYFLSVP
                     IERDIDVDTAARLANVSIDEFRMLNPQLDKPVILAAGTPQVLLPYDNADDFVRAVKAH
                     PGPLATWTVYSVGKTIRPADAARLVGMSETELREVNHIPARMLVRAGSTLLVPRSAQH
                     KADVSETVADSAAISLAPDLPPTRRVNMRVGRGGETVASVARRWHVSATQVAAWNHVS
                     AGGKFAAGSTAVLFLPRSTSLARAPSHPATRVAAKVKGGAKTRVAQAPNARSGTHVAV
                     SKAPPRKGKAVVAHKGGTPGKTVVTKTRVAQP"
     gene            complement(6213..6980)
                     /gene="gloB"
                     /locus_tag="VIN75_15405"
     CDS             complement(6213..6980)
                     /gene="gloB"
                     /locus_tag="VIN75_15405"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY8975604.1"
                     /translation="MDLIALPAFADNLIWMLHDGADAIVVDPGAASPVKNALRERGLR
                     LAAILVTHHHPDHVGGLAELQPLVQGPIYGPAHEPMPVAVEKMSHGMAFELLGLHVQV
                     LDVPGHTAGHIAYVATERGRAPWLFCGDTLFSGGCGRLFEGTPAQMQHSLSELAALPG
                     ESRVCCAHEYTLSNLRFARTVEPHNTALAEYEARCQALRAEGAWTLPSTIATERAVNP
                     FLRVREPEVVASARAHGAASGDPVDVLAALREWKNTF"
     gene            6979..7803
                     /locus_tag="VIN75_15410"
     CDS             6979..7803
                     /locus_tag="VIN75_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8975605.1"
                     /translation="METSAAIIELGEWLETPPGRVLLAWEQERIDRAVFDIFGYHALQ
                     MGLPSLDGLRANRMPHRWLASPSLVNPEPSPIAPAADLGFAISPLKVPVALHCCAEAL
                     PFASNSLDLVVMPHTLEMSSDPHQALAEATRCLVPDGKLVIFGFNPMSLWALRQRLGR
                     TGHRMRMADETLFLPSAGEFIGYWRLRDWLRLLSFDIEVGKFGCYRPPWSTQKWLDRY
                     GWMERVGERWWPVLGAVYFLVAVRRVRGMHLVGKLRKPPVKAATAPAVATINRGRR"
     gene            7866..8333
                     /gene="rnhA"
                     /locus_tag="VIN75_15415"
     CDS             7866..8333
                     /gene="rnhA"
                     /locus_tag="VIN75_15415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY8975606.1"
                     /translation="MPAMSESTTEVTIYTDGACKGNPGPGGWGCWLSASGKERELFGG
                     ATQTTNNRMELTAVIKGLSVLTRRCSVAVYTDSEYVRNGITSWIHGWKKRGWRTADGK
                     PVKNVELWQQLETLANAHDVQWHWVKGHAGDPGNERADALANRGVQAALNGQA"
     gene            complement(8429..9025)
                     /gene="mobA"
                     /locus_tag="VIN75_15420"
     CDS             complement(8429..9025)
                     /gene="mobA"
                     /locus_tag="VIN75_15420"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY8975607.1"
                     /translation="MAAPASRAAITGLVLAGGRGQRLGGVDKGLQPWHGRALVDHAIE
                     RLAPQVGGMMISANRNAADYASRGARVLADARDDFPGPLAGILAGLCAAATPWLAVVP
                     CDSPRLPADLVERLARGLDGRDGAVVQRDLGAAGVRLEPVCCLLSTSLAGDLARYLED
                     GGRKVEAWLSRHATPVRFDRPEDGAAFANINTPDDLAL"
     gene            complement(9025..10152)
                     /gene="moaA"
                     /locus_tag="VIN75_15425"
     CDS             complement(9025..10152)
                     /gene="moaA"
                     /locus_tag="VIN75_15425"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY8975608.1"
                     /translation="MGKTFAIHDLRRGPAPASVRAGTPLLAPGADRLDTFARPLRDLR
                     ISVTDRCNFRCGYCMPKEVFDSRHVFLPHAQLLSFEEIERLARLFADAGVRKLRLTGG
                     EPLLRKNLEHLVELLAALRTPDGLPLDITLTTNGSLLARKAAALKAAGLGRLTVSLDA
                     IDDAVFRRMNDVGFAVEDVLGGIDAALAAGFGPIKVNMVVKKGTNDDQVVPLTRALHA
                     RYGNATVLRFIEFMDVGSTNGWRLDDVLPSREVLERLREIGPLTALPASAAGETAQRW
                     RFDATGQEIGLVSSVTQAFCGDCNRARLSTDGQLYLCLFATHGHDLRALLRGGADDAA
                     IAGAIDQVWAARDDRYSALRTAATPAVDAGERRVEMHYIGG"
     gene            complement(10254..>10790)
                     /locus_tag="VIN75_15430"
     CDS             complement(10254..>10790)
                     /locus_tag="VIN75_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY8975609.1"
                     /translation="DCSGFTRYVFENSLGRVLPHRADEQANAPELAKVDRKDLKPGDL
                     VFFDTMRRTFSHVGIYLGDGKFIHSPRTGESVRIEDLNVSYWARRFTGGRRVPELADA
                     NGNPTGANTSMLASLTSRLPGFGGSGGAGGAPVVAPAIVSSTPALRLSSTPVALPAAA
                     PSALGPSHDPFARALTDR"
BASE COUNT         1494 a         3741 c         4066 g         1489 t
ORIGIN      
        1 gcgcatcgag gcgacgcgct cgcgcgacct gcagcgccgg gtcgatcgca tgcaggccat
       61 ccgcgcggag ctcgaccccg gcgtggcgcc gctgctgccg ggcgccggtg acggctcgcc
      121 cgacgcgctg gcggcgcgcg cgcaggcgct ggccgacgcc atcgacgccg ccgatcgcga
      181 gcagcgcgcg cgggacctgg cgcggctcac gcagatcccg ctggcgctgg cgcgccgcaa
      241 gggcgacgcg gacgacgcgg cgcatcgcct gccgccgccg cccgagacgg cgcaggacag
      301 gatcgcgcgc ctggagcgcc atgcccggga cctggccgag gcgcgccgcg cgcgcctgga
      361 cgcgcagcgc gagggccttc gcgtcacacg cgcgcacgtg aaggcgcacc gcatccatga
      421 acccgacgcc gcgctggcgc atacggcgcc ggcctcgggt tcgacgggcc aggcgcacgt
      481 ggccgacggc atcccgcgct cgatgaccga gcagttcaag tccatggtgc gcctgggcgg
      541 cccgcagggt cgcgtgggcg tggtcggcac gcaggccggg ctgtcggtca accgcgagca
      601 cgtcaagggc atcgccggcg gccgcaacgc caaggcggag gacttcgggc gcgacgccga
      661 cgtcgcgagc aaggggcgcc aggtcgtgct ggagggccag cgcgagaagg ccgggcaggg
      721 cggcggcatc ggcgaccacg gctcgggttc aagcaggcac ggcagcgccg cttcgacccg
      781 cggcgacgcg agcctgcgcg gcctcgcggc ggtgcgcatc ccgggcagca gcctcgacct
      841 cacgggccgg gtcgaggatg cccagcacac cttcgtgcgc tacgcctcgc caccggccgt
      901 cgatctggcc acgctgcgca ccgcggcggg ccgcgtgttc gggcccggcg gcacgcatgc
      961 ggagcgcgtc tacctcgaca cctggtacgt gatcggcccg ttcgcgggca agggcgcgga
     1021 gtcgcagcag gcgggctatc cgcccgagga cgacgtcgac ctcgatgccg cctatccggg
     1081 ccccgagggc cgcacgctga cgtggcagtt caccagccgc ggcttctacc cgttcgtgcc
     1141 gcccgaccgg cgcatggacg ccgtctacta cgcctacacc gagatccgcg tcgacgcgga
     1201 gcgcgacgtc tggttctccc tggccgccga cgacgactcg atgatgtggc tcgacgagcg
     1261 cctcgtgtgg atcagctcgc ccggcgacaa gccgtggtac cacccgccgt actacgcccg
     1321 cgacgagctc gtcggcagcc tgtcgctcac cgagggccgg cgccgcgtgc acctggcggc
     1381 cggcgtgcac cgcctgctgt tcaagctgtg caatgcggac ggccatccct tcttctccgt
     1441 cgtcctgtcc aagtgatcct catcatgcaa ttgaacctgt tccagcaact ggccaccggc
     1501 ggcgccggca tctgggtcat cgcggcgctg tcgctgctga tggtcgccgt ggcgatcgag
     1561 cgcttccatc gcttccgcat gtcggccatc gtgccaacgg ggctggtcga cgaggtcctg
     1621 ccgctgtggc aggccgggcg gctggaggaa ctgcagcagg cgctggcgcg gcgcgagagc
     1681 gtccttgcga tggcgatccg cttcgtcatc gccaaccggg cgcgctggac gcccgaggtg
     1741 ctcgacgagc gcgccggcga actcgcctcg cgcgcgctgc gccagcagca gcagcgcgcc
     1801 tacccgctgg cggtcgtggc gacggtggcg ccgatcgtgg gcctgctggg gaccgtggtc
     1861 ggcatgatcg aagccttcca cgtcatcgcg ttcacgggcg gcatgggcga cgcgtcgctg
     1921 ctggccggcg gcatctccaa ggcgctggtc aacaccgccg ccggcctcag cgtcgcactg
     1981 ccggcgctgg cgcttcatca tttcttccgc catcgcctga cgctgggtgg cctggcgctc
     2041 gaggagcaac tggaacgcgt gatggatgcc tgcgcgcgcg acggaggccg cgcatgagga
     2101 tcgccgtcgg cgaggacgag cagcccgaga tcagcctgat cgcgctgatc gactgcatct
     2161 tcttcctgct catgttcttc atggtcgcca cctcgttcca gaagcaggcc gagggcaagg
     2221 cggcgaggca gttgccgatc acgctgccgc agtccgaggt gtcgctggac gaggcgcagg
     2281 ccgacgcgag cccgctggtg atcggcgtcg acgtcaaggg cggcctgtac tggggccgcg
     2341 agccggtgtc gacggtgcaa ctgcgcgacc gcctgcacgc cgaggcgcgc aaggatccga
     2401 accgcgccgt gcgcatcgac ggcgataccc tggtgccgta ccaggacgtc gtgcacctgc
     2461 tggacctgtg cgagttcgaa gggctgacgc gcgtggcgct gcgcacgcgg gccgggcggt
     2521 gacgtcgggc gatgacacgc ctttggtccg ccagtgccgc ggcgtaccgc ggcccggccg
     2581 cagtaccttc cgctgctgcg cgaggactgg cgcgcctgcc ggccatgtcc ccggcaacgt
     2641 gatcgcgatg tcgcgacgcc ggttcgcgct acgatcccac cctcgacgcc gcacctcgcc
     2701 ctggacatga ccgacaccga actcgccgac ctctaccgcg actacatcgc ctgcctcaac
     2761 cggcgcgact ggcccgcgct cgcgcagttc gtctcgcacg acgtcatgca caacggccgg
     2821 ccgctgggcc tgtccggcta tcgggcgatg ctcgagaagg acgtggagga cgtgcccgac
     2881 ctgaagttca acgtcgagat ggtcacctgc gacccgccca acgtcgccgc gaagctcgcg
     2941 ttcgactgca cgcccaccgg ccaattccag ggcctggcgg tcaacggcaa gcaggtcagg
     3001 ttcaccgaga cggtgttcta cgagatcagc gaccgtcgca tcgccaccgt gtggtcggcc
     3061 atcgacaagg cggccatcga ggcgcagctc tgaacgcgcc gcgcgaacgg cgctaggagt
     3121 ccgcgcggct tctgccgcgc agactcctag ccgcgcagcg cggcgaggcg tcgatgcgcc
     3181 tcgtccgact cgtcgggcgg cacgctctgc ttcgcacaca ggtagcgttc gctgaacacg
     3241 tcgagccagg cctcgagcgt gcgcgcggcg cgggcgtcgc cggacaggtc caggcacagg
     3301 gcggcgatct ccgcggtgca ctggtgatgc tcgtgccagg cgctgcgcag gcggtagcgc
     3361 gagagcgtcg cggggtccag gcccagcacc gggaagcggt cgaggtacgg gctcttgcgg
     3421 aaggccttgc gcgcctcggc ccaggtgccg tccagcagga tgaacagagg acgccgcgcg
     3481 tcgcccggcg cgacggcggg cagtgcgccg acgacgcgct cgggcgccgc gaactccccg
     3541 ggaaacacga tgaagggttg ccagcgcggg tcgtccagca gcgccaccag cgccgggtcg
     3601 acgtgcgtgc gtgaccacgc gaacgcgtgc gtgtcgggca cgacgtcggc caccagccag
     3661 ccggtgttgc tgggcttgag cgcctcgaac ggcgccatca gcaggcacat gcccgcgcgc
     3721 gtgggcacgc gggggcggag tgcgcaggcg cagtgcgagg gcgcgaggcg gcagcgctcg
     3781 cagcgcgcct tgcgcggcgc gccgcgatcc aggcgcggcc gctgcgcgtc gtccgcgagg
     3841 caggcgtcgc gcaaccgggc gacggcgtgc ggcggcaggg cgtccttggc gggctcgttc
     3901 acgcgcgcat catgccgcac ccctgcgggc acgggctcag gcggccggct tctgcgccgc
     3961 ggccagcatc tcgcccatct tctggtggat gatgttgatg cccttgagcg ggcggatcat
     4021 caccttgaag tcgacgatct ggccggcgtc gttccacttg atgatgtcga cgccgttgac
     4081 gaggatgccg tcgatctcgg tctcgaactc gagcatcgcg tcatggggcc cggcgatctc
     4141 gcgcacgtag cggaacgtcg ggttgaagaa caccctgaag gcggccgaca ggtacatcac
     4201 cgtcagcttg cggcccggct gcgccgcgtg cacgaccggg gagtggaagg tggcgtcttc
     4261 cgcgatcagc gccgggatgc cggaggcgtc gttggtgcgc accaggcggt gccaggtctc
     4321 gaggacgggg gcggtcatgc gcgggcttcc ggtgaggttg gggcggccag tgtaggtcgc
     4381 gcccggcgca tgcggtcgag cgccgcctct agggtggtgt cagggctgcg cgacccgcgt
     4441 cttggtgacc accgtcttgc ccggcgtgcc gcccttgtgc gcgacgacgg ccttgccctt
     4501 gcgcggcggc gccttcgaga cggccacgtg cgtgccgctg cgcgcgttgg gcgcctgcgc
     4561 cacgcgcgtc ttcgcgccgc ccttgacctt ggcggcgacg cgggtggccg ggtgcgacgg
     4621 cgcgcgcgcc agcgacgtgg agcgcggcag gaacagcacg gccgtcgatc ccgcggcgaa
     4681 cttgccgccg gccgacacat ggttccaggc ggccacctgc gtggcgctga cgtgccagcg
     4741 gcgcgccacc gacgccaccg tctcgccgcc cctgcccacg cgcatgttga cgcggcgggt
     4801 cggcggcagg tcgggcgcca gcgagatggc agccgagtcg gcgaccgtct cggagacgtc
     4861 cgccttgtgc tgcgccgagc gcggcaccag cagcgtcgag ccggcccgca ccagcatgcg
     4921 cgccgggatg tggttgacct cgcgcagctc ggtctcgctc atgcccacca gcctggcggc
     4981 gtcggcggga cggatcgtct tgccgaccga gtacaccgtc cacgtcgcca gcggtcccgg
     5041 atgcgccttg accgcgcgca cgaagtcgtc ggcgttgtcg tagggcagca gcacctgcgg
     5101 cgtgccggcg gccaggatca cgggcttgtc cagctgcggg ttgagcatgc ggaactcgtc
     5161 gatggacacg ttggccagcc tggcggcggt gtcgacgtcg atgtcgcgct cgatgggcac
     5221 gctcaggaag tacgggtggt tgcccacggg cggcagcgtc agcgaatacg cctcggggtg
     5281 cagcacgatg ttcttgaccg cctgcagctt gggcacgtac tcgctggtct ccttgggcag
     5341 gttcaggctc gcgtagtcgg tgggcaggcc gcgcttctgg ttgcgcgcga tggcgcgctg
     5401 cacgttgccc tggccgcagt tgtaagcggc cagcgcgagc tgccagtcgc cgaacatgtc
     5461 gtgcaggcgc tgcaggtagt cgagcgcggc gcgggtggac gacagcacgt cgcggcggtc
     5521 gtcgcggaag atgttctgct tgaggtcgaa gtccttgccg gtggccgaca cgaactgcca
     5581 catgcccgag gccttggcca ccgacatcgc ttgcgggttg aacgcgctct cggtgaaggg
     5641 cagcagcgcc agttcggtcg gcatgtggcg gcgctcgacc tcctgggtga tgtggtacag
     5701 gtagcgcgcg ccgcgggccg tcatgcgggc cacgtagtcc gggcgcgacg agtaccactg
     5761 ctcccactgc ttgacggcgg gcgtgtccat gtcggggatc gcgaagccgt cgcgcacgcg
     5821 gtgccacagg tcggaacggg cggcgctgtc gtcgaggttg acgcgggtgt cgggctgcag
     5881 cgggtcgtac ggcggcagca cggccggggc ggtgcccgtc ttggccgcgt cgctgtcgtc
     5941 atcgtcgtcg gcctccatcg cctcggcgtc ggaggcggcc gcggccggcg cgctcgccga
     6001 gccggcggcg gccaaggcga ccgccggctg cgacgcggcg gcctcggaag cggacgccgc
     6061 gtcggccggc tgcatggcgg ccgtgacgac gggagcgggg ccggcggccg gcgcgggcgc
     6121 gaggtcgacg gtggagcagg ccgcgagcac cagcgcgagg gccagggaca cgacggtgga
     6181 cagcgacagg cgcgtcgggg gagcggcagt gttcaaaacg tgttcttcca ttcacgcagg
     6241 gcggctagga cgtcgaccgg gtccccggag gcggcgccat gggcccgcgc cgacgccacc
     6301 acctcgggtt cgcgcacgcg caggaacggg ttgacggcgc gttcggtggc gatggtcgac
     6361 ggcagggtcc aggcgccctc ggcgcgcagg gcctggcagc gggcctcgta ctcggccagg
     6421 gccgtgttgt ggggctcgac cgtgcgcgcg aaacgcaggt tcgagagcgt gtattcgtgc
     6481 gcgcagcaga cgcgcgactc gcccggcagc gcggcgagct cagacaggga gtgctgcatc
     6541 tgggcaggag ttccctcgaa cagccggccg cagccgccgg aaaagagcgt gtcgccgcag
     6601 aacagccagg gcgcgcggcc ccgctcggtg gcgacgtagg cgatgtggcc ggcggtatgg
     6661 ccgggcacgt ccagcacctg cacatgcagg ccgagcagct cgaacgccat gccgtgcgac
     6721 atcttctcga ccgccaccgg catcggctcg tgcgccgggc cgtagatcgg cccctgcacc
     6781 agcggttgca actcggccag gccgccgaca tggtccggat ggtggtgggt cactagaatg
     6841 gccgcgagcc gcagtccccg ttcgcgcaac gcgttcttca cgggcgacgc tgccccggga
     6901 tccaccacga tggcgtcggc gccgtcgtga agcatccaga tgaggttgtc ggcgaaggcg
     6961 ggtagggcga tcagatccat ggagacaagc gcagcgatta tagagttggg cgagtggctg
     7021 gagacaccgc ccggccgggt gctgctcgcc tgggagcagg agcggatcga ccgcgccgtc
     7081 ttcgacatct tcggctacca cgcgctgcag atgggcctgc cctcgctgga cggcctgcgc
     7141 gccaaccgca tgccgcaccg ctggctcgcc agccccagcc tggtcaatcc cgagccttcg
     7201 ccgatcgccc ccgcggccga cctcggcttc gccatctcgc cgctgaaggt gcccgtcgcg
     7261 ctgcattgct gcgccgaggc gctgccgttc gcctccaaca gcctcgacct ggtggtgatg
     7321 ccgcacacgc tcgagatgtc ctccgacccg caccaggcgc tggccgaggc cacgcgctgc
     7381 ctggtgcccg acggcaagct cgtgatcttc ggcttcaacc cgatgagcct gtgggcgctg
     7441 cgccagcgtc tcggccgcac gggccaccgc atgcgcatgg ccgacgagac gctgttcctg
     7501 ccgagcgccg gtgagttcat cggctactgg cgcctgcgcg actggctgcg gctgctcagc
     7561 ttcgacatcg aggtcggcaa gttcggctgc taccgcccgc cgtggagcac gcagaaatgg
     7621 ctggaccgct acggctggat ggagcgcgtg ggcgagcgct ggtggccggt gctgggcgcc
     7681 gtctacttcc tcgtggccgt gcggcgcgtg cgcggcatgc acctggtggg caagctgcgc
     7741 aagccgcccg tcaaggcggc gacggcgccg gcggtggcga cgatcaaccg ggggcggcgc
     7801 tgaagcgcgg acttgtccga acgggccagt gcacgtcgac gagggcgagc cgccacgaag
     7861 gtacgatgcc ggccatgagc gaatcgacga ccgaagtgac gatctacacg gacggggcct
     7921 gcaagggcaa ccccgggccg ggcgggtggg gctgctggct ctcggccagc ggcaaggagc
     7981 gcgagctgtt cggcggcgcc acgcagacga ccaacaaccg catggagctc accgcggtga
     8041 tcaagggcct gtcggtgctg acgcggcgct gctcggtggc ggtctacacc gacagcgaat
     8101 acgtgcgcaa tggcatcacg tcgtggatcc acggctggaa gaagcgcggc tggcgcacgg
     8161 ccgacggcaa gccggtgaag aacgtggagc tgtggcagca gctcgagacg ctggccaacg
     8221 cgcacgatgt gcagtggcac tgggtcaagg gccatgccgg cgatccgggc aacgagcgcg
     8281 cggatgcgct ggccaaccgg ggcgtgcagg cggcgttgaa tgggcaggcc tgaacggcgc
     8341 agggcgggtt ggcccgaggc gcggcccgcc atgtcggcca gtcggcaacg ccggcgaacg
     8401 gcggttgacg cctgcgactg acccgccctt acagcgccag gtcgtctggc gtgttgatgt
     8461 tggcgaaggc cgccccatcc tccgggcgat cgaagcgcac cggcgtcgca tggcgcgaca
     8521 gccaggcctc gaccttgcgg ccgccgtctt ccaggtagcg cgcgagatcg ccggccagcg
     8581 atgtcgacag caggcagcac accggctcca ggcgcacgcc cgcggcgccg aggtcgcgtt
     8641 gcacgacggc cccgtcgcgg ccgtccaggc ctcgggccag gcgctcgacg aggtcggcgg
     8701 gcaggcgcgg cgagtcgcag ggcaccaccg cgagccacgg cgtcgcggcg gcgcacaggc
     8761 ccgccaggat gcccgcgagc gggccgggga agtcgtcgcg cgcgtccgca agcacgcgcg
     8821 cgccgcgcga ggcgtagtcg gcggcgttgc ggttcgcgct gatcatcatg ccgccgacct
     8881 ggggcgcgag ccgctcgatc gcatggtcga ccagcgcccg gccatgccag ggctgcagcc
     8941 ccttgtcgac gccgcccagg cgctgcccgc ggccgccggc cagcaccagg ccggtgatcg
     9001 ccgcgcggga cgcaggcgcg gccatcagcc gccgatgtag tgcatctcga cgcgccgctc
     9061 gccggcgtcg accgcgggcg tggccgcggt gcgcagcgcg gagtagcggt cgtcgcgcgc
     9121 ggcccagacc tggtcgatcg cgcccgcgat ggcagcgtcg tcggcgccgc cgcgcagcag
     9181 cgcgcgcagg tcgtggccat gggtggcgaa caggcacagg tagagctggc cgtcggtgga
     9241 caggcgcgcc cggttgcagt cgccgcagaa ggcctgcgtg acgctggaga ccaggccgat
     9301 ctcctggccg gtggcgtcga agcgccagcg ctgggccgtc tcgcccgccg cgctggcggg
     9361 cagggccgtc aatgggccga tctcgcgcag gcgctcgagc acctcgcgcg agggcagcac
     9421 gtcgtccagt cgccagccgt tggtcgagcc gacgtccatg aactcgatga agcgcagcac
     9481 cgtggcgttg ccgtagcgtg catgcagggc gcgcgtgagc ggcaccacct ggtcgtcgtt
     9541 cgtgcccttc ttgacgacca tgttgacctt gatcggaccg aagccggcgg ccagcgcggc
     9601 gtcgatgccg ccgagcacgt cctcgaccgc gaagccgacg tcgttcatgc ggcggaacac
     9661 ggcgtcgtcg atggcgtcca ggctcaccgt gaggcggccc aggccggccg ccttcagcgc
     9721 cgcggccttg cgcgccagca gcgagccgtt ggtggtcagc gtgatgtcca gcggcaaccc
     9781 gtcgggtgtg cgcagggcgg cgagcagttc gacgaggtgc tccaggttct tgcgcagcag
     9841 cggctcgccg ccggtcagcc gcagcttgcg aacgccggcg tccgcgaaca ggcgcgcgag
     9901 ccgttcgatc tcctcgaagc tcagcagctg ggcatgcggc aggaacacat ggcggctgtc
     9961 gaacacctcc ttgggcatgc agtagccgca gcggaagttg cagcggtcgg tgaccgagat
    10021 gcgcaggtcg cgcagcgggc gcgcgaaggt gtcgaggcga tcggccccgg gcgcgagcag
    10081 gggggtgccc gcgcgcacgc tcgcgggcgc ggggccgcgt cgcaggtcgt ggatggcgaa
    10141 ggtcttgccc atggcccgat tgtgcccggc cggccggcat cccatcggcg tcgggtcatc
    10201 gcggaaacga cacggcccgc gcgaggcggg ccgtggcagg gagcacgatg tgttcagcga
    10261 tcggtcaggg cgcgcgcgaa cggatcgtgc gaggggccca gcgcgctggg cgcggccgcc
    10321 ggcagcgcga ccggcgtgct gctgaggcgc agggcgggcg tgctggacac gatggccggc
    10381 gccacgaccg gcgcgccgcc ggcaccgccc gatccgccga accccggcag gcgcgaggtc
    10441 agcgaggcca gcatcgacgt gttggcgccg gtcggattgc cgttggcatc ggccagctcg
    10501 ggcacgcggc ggccgccggt gaagcggcgc gcccagtacg agacgttcag gtcctcgatg
    10561 cgcacggatt cgccggtgcg cggcgagtgg atgaacttgc cgtcgcccag gtagatgccc
    10621 acgtgcgaga acgtgcggcg catggtgtcg aagaagacca ggtcgccggg cttgaggtcc
    10681 ttgcggtcga ccttggcgag ctcgggcgcg ttggcctgct cgtcggcccg gtgcggcagc
    10741 acgcggccca ggctgttctc gaagacgtag cgggtgaaac ccgagcagtc
//
LOCUS       DATCRO010000562         6742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_794072, whole genome shotgun sequence.
ACCESSION   DATCRO010000562 DATCRO010000000
VERSION     DATCRO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794072"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1833)
                     /gene="aceK"
                     /locus_tag="VIN75_15435"
     CDS             complement(25..1833)
                     /gene="aceK"
                     /locus_tag="VIN75_15435"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEY8975610.1"
                     /translation="MFPHQLDDPQAFAIARAIVEGFDRHYRLFTAAARGAKARFEKAD
                     WQGQLDAQRDRIAFYDLRVDEAVERLHREFRAGELSRETWHQVKLHTIGLLIDHHQPE
                     CCETFFNSVTTKILHRGDYRNDFIFVRPALSTDYLENDEPGATPTYRAYYPTRETLHE
                     TCMRVITNFQLEVEFQDLARDVADVVRALLGELKDARLRANFQVQVLSTLFYRNKGAY
                     VVGKIVNGFSELPFALPILHGDDGLLVIDTVLIGEEDLLLLFSAARAYFMVDMGVPSG
                     TVHFLRTLMPRKTRSELYAQVGLQKQGKALFYRDLLQHLRHSSDKFRIAPGIKGMVML
                     VFDLPSFPVVFKVIKDFFPPPKDTTPEQVEAKYLLVKQHDRVGRMADTLEFSNVALPR
                     HRFEEELIAELRQFCGSQLEDDGQTLVIRHAYIERRMVPLNIYLGDASPAQIEQVVVE
                     YGNAIKDLVAANIFPGDMLWKNFGVTRLGRVVFYDYDEIEYLTDCNFRRVPAPRNEEE
                     EMSGEPWYAVAPRDVFPETFGPFLLGNPAVRKVFMAHHADLLDAGYWQQQKDRILAGY
                     VHDVFPYDPSRRFSVQRAAAKAHPNSEARAMQPAPT"
     gene            complement(1851..2537)
                     /gene="can"
                     /locus_tag="VIN75_15440"
     CDS             complement(1851..2537)
                     /gene="can"
                     /locus_tag="VIN75_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEY8975611.1"
                     /translation="MSDDNSNDKDLSGLFEQNARWAEKMEQRSPGFFTSLLTQQTPQY
                     LWIGCADSRVPANELIGLLPGDVFVHRNVANVVAHSDLNALSVIQFAVDALKVRHIIV
                     VGHSKCGGVTAALNNSRVGVVDNWLRHVQDVRNAHMELLQAIPEAQRVDALVELNVLE
                     QARNVCLSTVVEDAWVRGQQVIVHGWVYGLHNGLLKDLSFTVASIDDVGPEYQRALFA
                     LRLRYLPAAA"
     gene            complement(2590..3291)
                     /locus_tag="VIN75_15445"
     CDS             complement(2590..3291)
                     /locus_tag="VIN75_15445"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8975612.1"
                     /translation="MTGAGAIACIGGGGFLVDDARGVQERFLLTLIPGSRRRERPRVL
                     YLGLAHGDAESRQLRAYKMFARLGCDITALPFFPYEMKRDYPAEALAADLVYVGGGNT
                     PAMIAVWREFGFDRALHDAWQAGTVLAGVSAGANCWFEDYVTDSVPGGGVRPGLGWLP
                     GTFCPHLDSEPWRAPLLAGVTRAPAVGVREGGMMIVRPGAAPEFVADRPGVTANVRLP
                     GEEAREVPALDLSSR"
     gene            complement(3350..3967)
                     /locus_tag="VIN75_15450"
     CDS             complement(3350..3967)
                     /locus_tag="VIN75_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975613.1"
                     /translation="MTRLPAPLPILQGERCLLRALVPQDAPALVAHADDPAVARNLFD
                     GFPQPYTMTAATAWATHESQSGDFGWVWAIVVDGALIGCIGLRPESGWLRCNAEIGYW
                     IGQAHWRRGITSDAVRQVTDWAFAAVPEITRIYAPIFADNAASQAVARTCGYVREATL
                     PQSAIKDGRVIDRVVWATYRTQTARRDADIDAALAATAASVMERA"
     gene            complement(3967..5148)
                     /locus_tag="VIN75_15455"
     CDS             complement(3967..5148)
                     /locus_tag="VIN75_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY8975614.1"
                     /translation="MSNLPGLNFMLGEDIDALRDAVRAFAQAEIAPRAAEIDRSDQFP
                     MDLWRKMGELGVLGITVPEEYGGANMGYLAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGSEAQKKKYLPRLISGEHVGALAMSEPGAGSDVISMKLKAEDKGGVYLLNGSK
                     MWITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHTGEL
                     VFQDVEVPAENVLGAVNGGAKVLMSGLDYERAVLTGGPLGIMQSVMDNVVPYIHDRKQ
                     FGQSIGEFQLIQGKVADMYTVLQAARSFAYTVAKNLDMLGAGHVRQVRKDCASVILWT
                     AEKATWMAGEGVQIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAET
                     M"
     gene            5299..5799
                     /locus_tag="VIN75_15460"
     CDS             5299..5799
                     /locus_tag="VIN75_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975615.1"
                     /translation="MPRDTPPANIRERPHLGVEVHDDSTLLRSQLALSSNWFAVGGMD
                     ITRRKGEVRLHRDGQVVARSDGNVLFLAGRPLHLRQAGFWRPVWTATLDDGRLVEVTT
                     GRHGIYKHVRVMPGDVRLEVVDTSSGFGRACRVNLVSGLIETELALGFLVYFLAELEA
                     AYRDRS"
     gene            complement(5807..6295)
                     /locus_tag="VIN75_15465"
     CDS             complement(5807..6295)
                     /locus_tag="VIN75_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8975616.1"
                     /translation="MSASPSTAGRNKAAKAPAAAKGAAKSFTITELAAEFDVTPRAIR
                     FYEDVGLLEPTRAGRNRVYSQRERTRLKLTLRGKRLGLSLQEIKQLVDMYDSPSDTRP
                     QLEAFLAVLRSHRRQIELQLEDIRVTLEEIAQHEARCERLLEAAGGAAAPAPAAKRAV
                     RA"
     gene            6345..>6742
                     /locus_tag="VIN75_15470"
     CDS             6345..>6742
                     /locus_tag="VIN75_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8975617.1"
                     /translation="MANADESKLAYPLGDTLPATGEVLDVAPGVRWLRMPLPFALDHI
                     NLWLLRDRADDGRAGWAIVDCGVANDATRAAWEQVLDRHLDGLPVLRVIVTHMHPDHL
                     GLAHWLTARWDCRLWISATDWHAARMACRST"
BASE COUNT          979 a         2329 c         2345 g         1089 t
ORIGIN      
        1 caatgctcct gaaaggatgg tgggtcaggt cggcgcgggc tgcatggccc tcgcttcgga
       61 attcggatgg gctttcgccg cggcgcgctg aacgctgaag cgccgcgacg ggtcgtacgg
      121 gaagacgtcg tggacgtagc cggcgaggat gcggtccttc tgctgctgcc agtacccggc
      181 gtccagcagg tcggcgtggt gcgccatgaa caccttgcgc accgccgggt tgcccagcag
      241 gaacgggccg aaggtctcgg gaaagacgtc gcgcggcgcc acggcgtacc agggctcgcc
      301 cgacatctct tcctcctcgt tgcgcggcgc cggcacgcgc cggaagttgc agtcggtcag
      361 gtactcgatc tcgtcgtagt cgtagaacac gacgcggccc aggcgcgtga cgccgaagtt
      421 cttccacagc atgtcgccgg ggaagatgtt ggccgcgacg agatccttga tcgcgttgcc
      481 gtactccacg accacctgct cgatctgcgc cgggctcgcg tcgcccaggt agatgttgag
      541 cggcaccatg cggcgctcga tgtaggcatg gcggatcacc agcgtctgcc cgtcgtcctc
      601 gagctggctg ccgcagaact gccgcagttc ggcgatcagc tcctcctcga agcggtggcg
      661 cggcagcgcg acgttggaga actcgagcgt gtcggccatg cggcccacgc ggtcgtgctg
      721 cttgacgagc aggtacttgg cctcgacctg ctcgggcgtg gtgtccttgg gcggagggaa
      781 gaagtccttg atgaccttga acaccaccgg gaacgacggc aggtcgaaca cgagcatcac
      841 catgcccttg atgccgggcg cgatgcggaa cttgtcgctg gaatggcgca ggtgctgcag
      901 caggtcgcgg tagaacagcg ccttgccctg cttctgcagc ccgacctgcg cgtacagctc
      961 ggagcgcgtc ttgcgcggca tcagcgtgcg caggaagtgc acggtgccgc tgggcacgcc
     1021 catgtccacc atgaagtagg cgcgcgccgc ggaaaacagc agcagcaggt cttcctcgcc
     1081 gatcagtacc gtgtcgatca ccagcaggcc gtcgtcgccg tgcaggatcg gcagcgcgaa
     1141 cggcagctcg ctgaagccgt tgacgatctt gccgacgacg tacgcgccct tgttgcggta
     1201 gaacagcgtc gacagcacct gcacctggaa gttggcgcgc aggcgggcgt ccttgagctc
     1261 gcccagcagc gcacgcacca cgtcggcgac gtcgcgcgcg agatcctgga actcgacctc
     1321 cagctggaag ttggtgatga cgcgcatgca cgtctcgtgc agcgtctcgc gcgtcgggta
     1381 gtaggcgcgg taggtcggcg tggcgccggg ctcgtcgttc tcgaggtagt cggtggacag
     1441 cgcgggccgc acgaagatga agtcgttgcg atagtcgccg cggtgcagga tcttggtcgt
     1501 caccgagttg aagaacgtct cgcagcactc gggctggtgg tggtcgatca gcaggccgat
     1561 cgtgtgcagc ttgacctggt gccaggtctc gcgcgacagt tcgccggcac ggaactcgcg
     1621 gtgcaggcgc tccacggcct cgtcgacgcg caggtcgtag aacgcgatgc ggtcgcgctg
     1681 cgcgtcgagc tggccctgcc agtcggcttt ctcgaagcgc gccttcgcgc cgcgcgccgc
     1741 cgcggtgaac agccggtagt ggcgatcgaa gccctcgacg atcgcccggg cgatcgcgaa
     1801 cgcttgcggg tcgtccagct ggtgcgggaa catcgcgtcc tcggatggct tcaggcggcg
     1861 gccggcaggt agcgcaggcg cagggcgaac agcgcgcgct ggtactcggg gccgacgtcg
     1921 tcgatgctgg cgacggtgaa gctcaggtcc ttgagcaggc cgttgtgcag gccgtagacc
     1981 cagccgtgca cgatcacctg ctggccgcgc acccaggcat cctcgaccac cgtcgacagg
     2041 caaacgttgc gcgcctgctc cagcacgttg agctcgacca gcgcgtcgac gcgctgcgcc
     2101 tcggggatgg cctgcagcag ctccatgtgc gcgttgcgca cgtcctgcac gtggcgcagc
     2161 cagttgtcga cgacgccgac gcgcgagttg ttcagcgccg ccgtcacgcc gccgcacttc
     2221 gagtgcccca cgacgatgat gtgcctgacc ttgagggcgt cgacggcgaa ctggatcacc
     2281 gacagcgcgt tcaggtccga gtgcgcgacg acgttggcca cgttgcgatg cacgaacacg
     2341 tcgcccggca gcaggccgat cagctcgttg gccggcacgc ggctgtcggc gcagccgatc
     2401 cacaggtatt gcggcgtctg ctgcgtcagc aggctcgtga agaagccggg gctgcgctgc
     2461 tccatcttct cggcccagcg cgcgttctgc tcgaacaggc cggagagatc cttgtcgttg
     2521 ctgttgtcgt cggacatggt catttcgtgt cgttgttggg cgaacggcca gtgtacggag
     2581 cggccggttt caccgcgagg acaagtcgag cgcggggact tcccgcgctt cttcgcccgg
     2641 caaccgcacg ttcgcggtga cgccgggcct gtcggcgacg aactcgggcg ccgcgccggg
     2701 acgcacgatc atcatcccgc cctcgcgcac gccgaccgcg ggggcgcgcg tcacgcccgc
     2761 gagcaggggc gcgcgccagg gctcgctgtc caggtgcggg cagaacgtgc cgggcagcca
     2821 gcccaggccc gggcgcacgc cgccgccggg cacgctgtcg gtgacgtagt cctcgaacca
     2881 gcagttggcg ccggcgctca cgcccgccag cacggtgccg gcctgccacg cgtcgtgcag
     2941 cgcgcggtcg aagccgaact cgcgccacac ggcgatcatc gcgggcgtgt tgccgccgcc
     3001 gacgtagacg aggtcggcgg ccagcgcctc ggcggggtag tcgcgcttca tctcgtacgg
     3061 aaagaacggc aacgccgtga tgtcgcagcc gagcctggcg aacatcttgt aggcgcgcag
     3121 ctgccggctc tccgcgtcgc cgtgcgcgag gcccaggtac agcacgcgcg gacgctcgcg
     3181 ccggcgcgac ccggggatca gcgtcagcag gaagcgctcc tgcacgccgc gcgcgtcgtc
     3241 gacgaggaag cccccgccgc cgatgcaggc gatcgcgccg gcgcccgtca tcggatggcc
     3301 tccgcgctgc gcgctgcggc gcgaacgcct tggggcggcc gggcggcgtt catgcccgct
     3361 ccatcacgct ggccgccgtg gcggcgagcg cggcgtcgat gtccgcgtcg cgtcgcgccg
     3421 tctgcgtgcg gtaggtcgcc cacaccacgc ggtcgatcac gcggccgtcc ttgatcgcgc
     3481 tttgcggcag cgtcgcttcg cgcacgtagc cgcaggtgcg cgcgaccgcc tgcgaagctg
     3541 cgttgtccgc gaagatcggc gcgtagatgc gcgtgatctc cggcaccgcc gcgaacgccc
     3601 agtcggtgac ctggcgcacc gcgtcgctgg tgatgccgcg ccgccagtgc gcctggccga
     3661 tccagtagcc gatctcggca ttgcagcgca gccagccgct ttccggccgc aggccgatgc
     3721 agccgatcaa cgcgccgtcc acgacgatcg cccagaccca gccgaagtcc cccgactgcg
     3781 actcgtgtgt cgcccaggcg gtggccgcgg tcatcgtgta gggctgcggg aagccgtcga
     3841 acaggttgcg tgcgacggcc ggatcgtcgg cgtgcgcgac cagcgccggc gcgtcctgcg
     3901 gcacgagcgc gcgcagcagg cagcgctcgc cctgcaggat gggaagcggc gccggcagcc
     3961 gggtcatcac atcgtctcgg cgaacagctc gcggccgatc agcatgcggc ggatctcgct
     4021 ggtgcccgcg ccgatctcgt agagcttggc atcgcgccac aggcggccca gcgggtactc
     4081 gttgatgtag ccgttgccgc cgaagatctg cacgccctcg ccggccatcc atgtcgcctt
     4141 ctcggccgtc cacaggatga ccgaggcgca gtccttgcgc acctggcgca catgcccggc
     4201 accgagcatg tccaggttct tggccaccgt gtaggcgaag ctgcgcgcgg cctgcagcac
     4261 ggtgtacatg tcggccacct tgccctggat gagctggaac tcgccgatgc tctggccgaa
     4321 ctgcttgcgg tcgtggatgt aagggacgac gttgtccatc accgactgca tgatgcccag
     4381 cgggccgccg gtcagcaccg cgcgctcgta gtccaggccc gacatcagca ccttggcgcc
     4441 gccgttgacc gcgcccagca cgttctcggc cggcacctcg acgtcctgga acaccagctc
     4501 gccggtgtgg ctgccgcgca tgcccagctt gtcgagcttc tgcgcgatcg agaagccctt
     4561 catgcctttc tcgatcagga aggccgtgac gccgcgcgcg cccagctcgg gctcggtctt
     4621 ggcgtagacc accagcgtgt cggcgtcggg gccgttggtg atccacatct tgctgccgtt
     4681 gagcaggtag acgccgccct tgtcctcggc cttgagcttc atcgagatga cgtcggagcc
     4741 ggcgccgggc tcgctcatcg ccagcgcgcc cacgtgctcg cccgagatca gcctgggcag
     4801 gtacttcttc ttctgcgcct cggagccgtt gcgcttgatc tggttgacgc acaggttgga
     4861 gtgcgcgccg tagctcaggc ccaccgaggc gctggctcgg ctgatctcct ccatcgcgat
     4921 catgtgcgcg aggtagccca tgttggcgcc gccgtattcc tcgggcacgg tgatgcccag
     4981 cacgcccagc tcgcccatct tgcgccacag gtccatcggg aactggtcgc tgcggtcgat
     5041 ctcggccgcg cgcggcgcga tctcggcctg cgcgaacgcg cggacggcgt cgcgcagcgc
     5101 gtcgatgtct tcgccgagca tgaagttcag gccgggcagg ttgctcatgg tgtcgtctcc
     5161 aggatcagga cgcgccgccg acgggcgaac gccattgttg ctccgggctg cgcacccggc
     5221 gaggccgtcg agtttaattg acgtttacgt aaacgtaaac cgagatcacc cggagagggc
     5281 atgcccgaca atccgcgcat gccccgcgac accccgcccg ccaacatccg cgaacggccg
     5341 cacctgggag tcgaggtgca cgacgactcg acgctgctgc gcagccagct cgcgctctcg
     5401 tccaactggt tcgcggtggg tggcatggac atcacccgac gcaaggggga agtccggctc
     5461 catcgcgatg gccaggtcgt tgcccggagt gacggcaacg tgctgtttct cgccggccgc
     5521 ccgctgcacc tgcgccaggc cggattctgg cgccccgtct ggacggcgac cctggacgac
     5581 gggcgcctgg tcgaggtgac gacaggccgg cacggcatct acaagcacgt gcgcgtgatg
     5641 ccaggcgatg tgcggctgga agtggtcgac acgagcagcg gctttggccg ggcctgccgc
     5701 gtcaacctgg tgtccgggct gatcgagacc gagctggcgc tcggctttct cgtctacttc
     5761 ctggccgaac tggaagcggc ctaccgcgac aggagctgaa cgcccgtcac gcacgcaccg
     5821 cccgcttggc ggcgggcgcg ggcgccgcgg cgccgccggc cgcctccagc aggcgctcgc
     5881 agcgcgcctc gtgctgggcg atctcctcca gcgtgacgcg gatgtcctcc agctgcagct
     5941 cgatctgccg gcggtgcgac cgcagcaccg ccaggaacgc ctccagctgg ggccgggtgt
     6001 ccgacggcga gtcgtacatg tccaccagct gcttgatctc ctgcaggctc aggcccaggc
     6061 gcttgccgcg cagcgtcagc ttcagccggg tgcgttcgcg ctgcgaatag acacggttgc
     6121 gtccggcccg cgtgggctcc agcaggccga cgtcctcgta gaaacggatc gcgcgcgggg
     6181 tgacgtcgaa ctcggcagcc agctccgtga tcgtgaagct cttggccgcg cccttggcgg
     6241 cggcgggcgc cttcgccgcc ttgttcctgc ctgcggtact gggagatgcg ctcatggtta
     6301 gactgacgtt aacgtaaacg gaagtctagt ccaccggagc ccgcatggcc aacgccgacg
     6361 aatcgaaact ggcctacccg ctgggcgaca cgctgcccgc gacgggcgag gtgctggacg
     6421 tcgcgccggg cgtgcgctgg ctgcgcatgc cgctgccgtt cgcgctcgac cacatcaacc
     6481 tgtggctgct gcgcgatcgc gcggacgacg ggcgcgcggg ctgggcgatc gtcgactgcg
     6541 gcgtcgccaa cgacgccacg cgcgccgcct gggagcaggt cctcgaccgc catctcgacg
     6601 gcctgcccgt gctgcgcgtg atcgtcacgc acatgcatcc ggaccatctc ggcctggcgc
     6661 actggctcac ggcgcgctgg gactgccggc tgtggatcag cgccaccgac tggcacgccg
     6721 cgcgcatggc gtgccgttcg ac
//
LOCUS       DATCRO010000563         9470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_433813, whole genome shotgun sequence.
ACCESSION   DATCRO010000563 DATCRO010000000
VERSION     DATCRO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433813"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1136
                     /locus_tag="VIN75_15475"
     CDS             408..1136
                     /locus_tag="VIN75_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8975618.1"
                     /translation="MIDFIIGLAIGAALAGAFAAWRGSRAARTLAPDVEAQRLDVPDH
                     SVAVRAAEEALRTQQAEFARQRKDGEARMAQMQREHDQLLRDVGARVAEVKTSALRNC
                     DSLAGEINQLLGVVKTFERWHAEMNTLLAHNREMHAKSEEFSKIVEQVVIVALNASIE
                     AARAGDQGRGFAVVANEIRTLAGRAERLSKDYRANLYKNDLITTTTFQDLQAGGKMIV
                     GAVIGLELINNKSKDVLTAEAAVA"
     gene            1133..1687
                     /locus_tag="VIN75_15480"
     CDS             1133..1687
                     /locus_tag="VIN75_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975619.1"
                     /translation="MITERAKASFDRLLVSSLRSGMPVGGASVSVEPVATIEKNKDKK
                     IVVLTVSSYLFRALAIIYFKPDAATRAYFNRNAEEGAPALGDEEFYDRVAECGNVCVG
                     ALNRDLGPFFPHVGMSTPNIIDKDCMQYVDLLGCGLVRHYRVVLAGELTLHATLCVAD
                     YGLVDFHVDPAVEVEVDTGELEMF"
     gene            1730..2098
                     /locus_tag="VIN75_15485"
     CDS             1730..2098
                     /locus_tag="VIN75_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975620.1"
                     /translation="MAQILVVDDSSTVRDEVAGFLKRNGLDVATAVDGKDGLSKLRMD
                     PKLKLVVSDVNMPNMDGLTMAEKIRGELGNKTVNIVMLTTENSPIMKERGKAAGIKGW
                     IVKPFKGDAVLATFKKLVEA"
     gene            2104..2247
                     /locus_tag="VIN75_15490"
     CDS             2104..2247
                     /locus_tag="VIN75_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975621.1"
                     /translation="MAANRPHRPARAAAQARRQPAFLRLAAGLWIGAVLAAFSAIHFL
                     VMP"
     gene            2244..4565
                     /locus_tag="VIN75_15495"
     CDS             2244..4565
                     /locus_tag="VIN75_15495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975622.1"
                     /translation="MSTTTPLEHAAVERAAASAAAHDAPPAPAAGAGSSVAMKKILAM
                     VAVAIACFLVISSYQVVKSVESKTSLTKVEQSYFPVLIKADSSIAVMEKVSDDYVKAV
                     MTAAADKAVAAHELGKGAVGNLREMGALFPDKARQIDQIRTQFNAYNEEAARMDDDYL
                     NHRMVLRDWLPRIKEMNENLATTRTKLKELRAAFYDDFSRSVAKTQSAMQWNLALSLA
                     LGTMNIAFMAVLVFLIRNNLRMMGQISDHNANLERRVTERTLALRQKTADIQSMLQNM
                     PQGVLTVVAGNKVHAEYSAYLETIFETASIADRDVMALVFANTSLGADTLSTIEVSIA
                     SCIGEDAMNYEFNSHLLVTEFDLRMADGRVKSLELSWSPIVGDGDVVEKLMLCVRDVT
                     ELKRLANEASTQRRELEMIGEILSVSQEKFQDFMQTSLAFVEENRALTEQATGPTAEL
                     ITQLFRNMHTVKGNARTYGLGNLTNTVHEAEQTYDELRKGQTTEWHPETLLQQLDSVR
                     GLLDEYAKINDTKLGRKGPGRRGNVEKFLMVDKSQVTQTLQAMQSVDMDDVDAMRAAL
                     LETQKLLRRIGTERLQDTLGGVVESLPSLARELGKEPPVVSLHDDGILVRNQVTGLLK
                     NLFTHLLRNAIDHGIEAPAARVAAGKAAVGRIDLDAAIAGDEFRMVLRDDGQGMALDR
                     IRAIAAERGLVEASASLSDEETAQLIFLPGFSTAQVVTEVSGRGVGMDAVKAFLDRER
                     GRIDVRFLGERNAAGNRPFEFAIVLPAAFAVAA"
     gene            4614..5972
                     /locus_tag="VIN75_15500"
     CDS             4614..5972
                     /locus_tag="VIN75_15500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY8975623.1"
                     /translation="MPHTPALARLAVAIAIGSSTPAFAADSPDATLGAIADAVDPRAL
                     HATIEKLVGFGTRHTLSDTKSDTRGIGAARRWTQARFEAISKECGGCLEIVTPSQHVT
                     GKRAPDGVDIVDVVAIQRGTSDPERVIAITGHLDSRVTDVMDATHDAPGADDDGSGTA
                     AVMESARVLSKHKFAGTIVYGVLSGEEQGLYGGKVLADYAKARGWKVEADLNNDIVGN
                     SHGQDGVNDNGVIRVFSEGTKAVETPEQAGKRRYNGGELDSPSRNLARFVAGLAEHHL
                     VNFRVNMVYRTDRYGRGGDQVPFLEAGFPALRFTEPHEDYRNEHQDLRTADGVRFGDT
                     IDRVDFDYLAQVTRLNAITLAALANAPAPVKGVAIGGAVTTDTVMTWTPQPGVAAYRV
                     WWRDTTAPQWQRSRTYPANVDPGRAVLRNIDLDDFFFGVSAISADGWESPVTFPGDAG
                     SF"
     gene            complement(6250..6744)
                     /locus_tag="VIN75_15505"
     CDS             complement(6250..6744)
                     /locus_tag="VIN75_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY8975624.1"
                     /translation="MIALIRTLPLHAARYKDYTARRRVTSFGGSYDFDANKLLPAQPL
                     IEQLHPLRARVAHWAGIEPEALRHVLVAEYAPGTPLGWHRDVPDHEDVFGVSLGSTAV
                     LRFRPYPPKDPKRADIVKLRAAPRSVYALRGPARWAWQHSVAPVEELRWSITLRTARV
                     GRRS"
     gene            complement(6849..7289)
                     /locus_tag="VIN75_15510"
     CDS             complement(6849..7289)
                     /locus_tag="VIN75_15510"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEY8975625.1"
                     /translation="MSSRPSAIPTAPCTNAERLLPGHQFADAYKVPAPYGVDAIEATR
                     LAFARGPLWIRSLLGLRNGLVRLVGLKPARSRGFPVLSETPREVVMGFDDRHLDFRIV
                     VVVAGGFATVTTLVRWHNAWGRAYLAVVLPFHRAIAARMIEGVA"
     gene            7406..8596
                     /locus_tag="VIN75_15515"
     CDS             7406..8596
                     /locus_tag="VIN75_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8975626.1"
                     /translation="MNLHDYDAWLAPLASALGAALVGILVHVMLRPIVRRLARHAPVA
                     NAVVQQIDRPVRWLLPLMAVQVVLEGAPDDLPHMGGMRQLVTVLLIAAFTATAIAAVR
                     GVAEAVALLHPQDVADNLQARRILTQTRVLARIAMGIALFAGVAFILMTFPRARQFGT
                     SLLASAGLSALVIGLAAKSVFGNLLAGLQIALSQPLRIDDVLIVQGEWGRVEEITSTY
                     VVLKIWDERRLVIPLQWFIDNPFQNWTRTTSQIIGSVFFWVDYTTDLAPLRAEATRLA
                     QGSKDHDGRVCLLQVVDTSERAMKLRLIVSSPSAGQSWDLCCLLREGLVALMQREHPD
                     ALPRLRAEMREAARPHDHRDELDARIAGQRTDPGRAQVNNDTGLTSLTAPTGGVLPPA
                     EPPR"
     gene            8901..>9470
                     /locus_tag="VIN75_15520"
     CDS             8901..>9470
                     /locus_tag="VIN75_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975627.1"
                     /translation="MKRRTMLSLLASLPAAPLFAKESGTLVLGQSAPNTGPSAQLGIQ
                     LNRGARLYFDKVNAAGGVNGQKIELRMLDDGYEPARAKANTEKFLNDDVFALFGYVGT
                     PTSVACLPMVKDAGIPFFGPFTGAMALRQPALRNVFHVRASYDDETALIVNQLHNLGL
                     KKIAVFKQNDAYGQAGLDGVNKALKALDLA"
BASE COUNT         1582 a         3353 c         3166 g         1369 t
ORIGIN      
        1 ggtctcgtgg ccgaccacga acggcgcctc gagccgaacg cggaaccacg cgccgaactc
       61 ggtggcatcg gtgatcgcgc gccacacgcg cgacggcggc gccttgatga gcttctgttg
      121 gcggatctcg ttggtgctcg acatgcaggt ctcctggacg tggccggttg tacgcccgat
      181 cgtccgttca tgcaaccgcc gggttgcctt ttcatgatcg aacgttcatg gtgcagcgca
      241 gcggacatcg gtcacgcttc gcgcgggatc gcggcggtcg ggtcgcacga cggtcacgaa
      301 tggactcaaa agcgcccgct gatggacgaa atcccctgaa tgcggcaagc ggagcatttc
      361 gtgctgccgg cgagcttcgc cgcgacggcc tgaaaaggga cattgacatg atcgacttca
      421 tcatcggcct cgcgatcggc gcggccctgg ccggcgcctt cgcggcgtgg cgcggcagcc
      481 gcgccgcgcg caccttggcg ccggacgtcg aggcacagcg cctggacgtg ccggatcact
      541 cggtcgccgt gcgcgccgcc gaggaggcgc tgcgcacgca gcaggccgag ttcgcgcgcc
      601 agcgcaagga cggcgaggcg cgcatggcgc agatgcagcg cgagcacgac cagctgctgc
      661 gcgacgtcgg cgcgcgcgtg gccgaggtca agacgtcggc gctgcgcaac tgcgactcgc
      721 tggccggcga gatcaaccag ctgctgggcg tcgtcaagac cttcgagcgc tggcacgccg
      781 aaatgaacac gctgctcgcg cacaaccgcg agatgcacgc caagagcgag gagttctcca
      841 agatcgtcga gcaggtcgtg atcgtcgcgc tgaacgcgtc gatcgaggcc gcgcgcgccg
      901 gcgaccaggg ccgcggcttc gcggtcgtcg ccaacgagat ccgcacgctg gccggccgcg
      961 ccgagcgcct gtccaaggac taccgcgcca acctctacaa gaacgacctg atcaccacca
     1021 ccacgttcca ggacctgcag gccggcggca agatgatcgt cggcgcggtg atcggcctcg
     1081 agctgatcaa caacaagtcc aaggacgtgc tgaccgccga ggcggccgtc gcatgatcac
     1141 cgaacgcgcc aaggcgagct tcgaccgcct gctggtctcg tcgctgcgca gcggcatgcc
     1201 cgtcggcggc gcgagcgtca gcgtggagcc cgtggccacg atcgagaaga acaaggacaa
     1261 gaagatcgtc gtcctgaccg tctcgtccta cctgttccgc gcgctggcga tcatctactt
     1321 caagcccgac gccgccaccc gggcgtactt caaccgcaac gcggaggaag gcgcgccggc
     1381 gctgggcgac gaggagttct acgaccgcgt ggccgagtgc ggcaacgtgt gcgtgggcgc
     1441 gctcaaccgc gacctcgggc cgttcttccc gcacgtgggc atgtccacgc ccaacatcat
     1501 cgacaaggac tgcatgcagt acgtcgacct gctgggctgc ggcctcgtca ggcactaccg
     1561 cgtcgtcctt gccggcgagc tcaccctgca cgccacgctc tgcgtcgccg actacggcct
     1621 cgtcgatttc catgtcgacc cggccgtcga ggtcgaggtc gacaccggcg agctcgagat
     1681 gttctgaatt ccccgtccct gccaccaccg aaactccgga gcgctcccca tggcccagat
     1741 cctggtcgtc gatgattcca gcaccgttcg cgatgaagtc gcgggcttcc tcaagcgcaa
     1801 cggcctggac gtcgccacgg ccgtcgacgg caaggacggc ctgtccaagc tgcgcatgga
     1861 ccccaagctc aagctcgtcg tctccgacgt caacatgccc aacatggatg gcctgacgat
     1921 ggccgagaag atccgcggcg agctcggcaa caagacggtc aacatcgtga tgctgaccac
     1981 cgagaacagc cccatcatga aggagcgcgg caaggccgcc ggcatcaagg gctggatcgt
     2041 caagccgttc aagggcgacg ccgtgctggc gacgttcaag aagctggtcg aggcctgaac
     2101 atcatggccg cgaaccgccc gcatcgcccc gcccgcgccg ccgcgcaagc ccgtcgccag
     2161 ccggccttcc tgcgcctggc cgcgggactc tggatcggcg cggtgctcgc cgccttttcc
     2221 gccatccact tcctggtgat gccatgagca cgaccacacc cctggagcac gccgcggtcg
     2281 aacgcgccgc ggcctccgcc gccgcgcacg acgcgccgcc cgcccccgct gcgggcgccg
     2341 gcagcagcgt cgcgatgaag aagatcctcg ccatggtggc ggtggccatc gcctgcttcc
     2401 tggtgatctc cagctaccag gtcgtgaagt cggtcgagtc caagacctcg ctcacgaagg
     2461 tggagcaatc ctatttcccc gtgctgatca aggccgattc gtccatcgcg gtgatggaga
     2521 aggtcagcga cgactacgtc aaggcggtga tgaccgcggc cgccgacaag gccgtcgcgg
     2581 cgcacgagct cggcaagggc gcggtgggca acctgcggga gatgggcgcg ctgttcccgg
     2641 acaaggccag gcagatcgac cagatccgca cgcagttcaa cgcgtacaac gaggaagccg
     2701 cccgcatgga cgacgactac ctcaaccacc gcatggtgct gcgcgactgg ctgccgcgca
     2761 tcaaggagat gaacgagaac ctggccacga cgcgcacgaa gttgaaggaa ctgcgcgccg
     2821 ccttctacga cgacttctcg cgctcggtgg ccaagacgca gtcggcgatg cagtggaacc
     2881 tcgcgctgag cctggcgctc ggcacgatga acatcgcgtt catggcggtg ctcgtcttcc
     2941 tcatccgcaa caacctgcgc atgatgggcc agatctccga ccacaacgcc aacctggagc
     3001 gccgcgtcac ggaacgcacg ctggccctgc gccagaagac ggccgacatc cagagcatgc
     3061 tgcagaacat gccccagggc gtgctcacgg tggtggccgg caacaaggtg cacgccgagt
     3121 actcggccta tctggagacc atcttcgaga cggcgagcat cgccgaccgc gacgtcatgg
     3181 cgctggtgtt cgcgaacacc tcgctgggcg ccgacacgct gtcgaccatc gaggtctcga
     3241 tcgcctcgtg catcggcgag gacgcgatga actacgagtt caactcgcac ctgctggtca
     3301 ccgagttcga cctgcgcatg gccgacggcc gcgtcaagtc gctggagctg agctggtcgc
     3361 ccatcgtggg ggacggcgac gtcgtcgaga agctgatgct gtgcgtgcgc gacgtcaccg
     3421 agctcaagcg cctggccaac gaggcctcca cgcagcgccg cgagctcgag atgatcggcg
     3481 agatcctgtc ggtgtcgcag gagaagttcc aggacttcat gcagacctcg ctggccttcg
     3541 tcgaggagaa ccgcgcgctg accgaacaag ccaccggccc gaccgcggag ctgatcacgc
     3601 agttgttccg caacatgcac acggtcaagg gcaacgcccg cacgtacggc ctgggcaacc
     3661 tgaccaatac ggtccacgaa gccgagcaga cctacgacga gctgcgcaag ggacagacca
     3721 ccgagtggca ccccgagacg ctgctgcagc agctcgactc ggtgcgcggg ctgctggacg
     3781 agtacgccaa gatcaacgac accaagctgg gtcgcaaggg cccgggccgg cgcggcaacg
     3841 tcgagaagtt cctgatggtc gacaagtccc aggtcacgca gacgctgcag gcgatgcagt
     3901 cggtggacat ggacgacgtc gacgcgatgc gcgccgcgct gctggagacg cagaagctgt
     3961 tgcgccgcat cggcacggag cgcctgcagg acacgctggg cggcgtcgtc gaatcgctgc
     4021 cctcgctggc cagggagctc ggcaaggagc cgccggtcgt ctcgctgcac gacgacggga
     4081 tcctggtgcg caaccaggtc actggcctgc tcaagaacct gttcacgcac ctgctgcgca
     4141 acgcgatcga ccacggcatc gaggcgccgg ccgcgcgcgt ggccgcgggc aaggcggcgg
     4201 tcggccgcat cgatctggac gcggcgattg ccggtgacga gttccggatg gtgctgcgcg
     4261 acgacggcca gggcatggcg ctcgatcgca tccgcgcgat cgccgccgag cgcggcctgg
     4321 tcgaggccag cgccagcctc agcgacgagg agacggcgca gctgatcttc ctgccggggt
     4381 tctcgacggc ccaggtggtg accgaggtct cggggcgcgg cgtgggcatg gatgccgtca
     4441 aggccttcct cgaccgcgag cgcggacgca tcgacgtgcg cttcctcggc gaacgcaacg
     4501 ccgcgggcaa ccgcccgttc gagttcgcga tcgtgctgcc tgccgcgttc gccgtcgcgg
     4561 cctgatcacc cgcctggcgg cgcgggcggc gcggcgtgcc cataatcggc ggcatgccgc
     4621 acacccccgc cctcgcccgc ctcgcggtgg ccatcgccat cggctcgtcc acgcccgcgt
     4681 tcgccgcgga ttcgcccgac gcgacgctcg gagcgatcgc cgacgcggtc gacccgcgcg
     4741 cgctgcacgc caccatcgag aagctggtgg gcttcggcac gcggcacacg ctgtcggaca
     4801 cgaagtcgga cacccgcggc atcggcgccg cgcgccgctg gacgcaggcc cgcttcgaag
     4861 cgatctccaa ggagtgcggc ggctgcctcg agatcgtcac gccgtcgcag cacgtgaccg
     4921 gcaagcgcgc gcccgacggg gtcgacatcg tcgacgtcgt cgcgatccag cgcggcacat
     4981 cggaccccga acgcgtgatc gcgatcaccg gccacctcga ttcacgcgtg accgacgtga
     5041 tggacgcgac gcacgacgcg cccggcgccg acgacgacgg ctccggcacg gccgccgtca
     5101 tggagtcggc gcgcgtcctc tcgaagcaca agttcgccgg caccatcgtc tacggcgtgc
     5161 tgtccggcga ggagcagggc ctgtacggcg gcaaggtgct cgccgactac gccaaggcca
     5221 ggggctggaa ggtcgaggcc gacctgaaca acgacatcgt cggcaacagc cacggccagg
     5281 acggcgtcaa cgacaacggc gtgatccgcg tgttctccga aggcaccaag gcggtcgaga
     5341 cgccggagca ggccggcaag cgccgctaca acggcggcga gctggactcg ccgtcgcgca
     5401 acctggcgcg cttcgtggcg gggctggccg aacaccacct cgtcaacttc cgcgtcaaca
     5461 tggtctaccg caccgaccgc tacggccgcg gcggcgacca ggtacccttc ctcgaggccg
     5521 gctttcccgc gctgcgcttc accgagccgc acgaggacta ccgcaacgag caccaggacc
     5581 tgcgcaccgc ggacggcgtg cgcttcggcg acacgatcga ccgcgtcgac ttcgactacc
     5641 tcgcgcaggt cacgcgcctg aacgccatca cgctcgcggc gctggccaat gcgccggcgc
     5701 cggtcaaggg cgtggcgatc ggcggcgcgg tcaccaccga caccgtcatg acctggacgc
     5761 cgcagcccgg cgtggccgcc taccgcgtgt ggtggcgcga caccaccgcg ccgcagtggc
     5821 agcgcagccg cacctatccg gccaacgtcg accccggccg cgcggtgctc aggaacatcg
     5881 atctcgatga tttcttcttc ggcgtgtcgg cgatctcggc cgatggctgg gagtcgccgg
     5941 tcaccttccc gggggatgcg ggcagcttct gatccaccgc gaggtcaggt cttgaatgtt
     6001 gcgcgttgaa ttgcatggcc tcggggtcag gtcttgaaag ttgcgcggca aggcttctgg
     6061 catcggggtc aggtcttgaa agttgcgcgg caaggcttcg gcgggcgctg acggctcaac
     6121 gcgcaacttt caagacctga ccccaaggcg agatgtcgcc gcgcaatttt caagacctga
     6181 ccccaaggcg agatgtcgcc gcgcaacttt caagacctga ccccaaggcg agatgtcgcc
     6241 gcgcaacatt caagacctgc gccccactcg agccgtccgc agcgtgatcg accaccgcaa
     6301 ctcctccacc ggcgcgacgc tgtgctgcca cgcccagcgc gccgggccgc gcagcgcgta
     6361 gaccgagcgc ggtgccgcgc gcagcttgac gatgtccgcg cgcttggggt ccttgggcgg
     6421 ataggggcgg aagcgcagca ccgcggtcga gccgagcgag acgccgaaga cgtcctcgtg
     6481 gtcgggcacg tcgcggtgcc agcccagcgg cgtgcccggc gcgtactcgg ccaccagcac
     6541 gtgccgcagc gcctcgggct cgatgcccgc ccagtgcgcg acacgcgcgc gcagcggatg
     6601 cagttgctcg atcagcggct gcgccggcag cagcttgttg gcgtcgaaat cgtagctgcc
     6661 gccgaagctc gtcacgcggc ggcgcgccgt gtagtccttg tatctcgcgg cgtgcagcgg
     6721 cagcgtgcgg atcagcgcga tcaacgcctg ctcctcctcg cgcgagagga actccggctc
     6781 gtaggccagc ccctcgggca ggccggcggg cagcggctcg ggcaggtcgg gaaagaggga
     6841 cggctggttc aggcgacgcc ctcgatcatg cgcgccgcga tggcccggtg gaacggcagg
     6901 acgacggcca ggtaggcgcg gccccaggcg ttgtgccagc gcacgagcgt ggtgacggtg
     6961 gcgaaaccgc cggcgaccac gacgacgatg cggaagtcga ggtgccggtc gtcgaagccc
     7021 atcacgactt cgcgcggcgt ctcggacagg acaggaaagc cgcgcgagcg cgccggcttc
     7081 agccccacca gccgcaccag gccgttgcgc agcccgagca gcgagcggat ccacagcggg
     7141 ccgcgcgcga acgcgaggcg cgtcgcctcg atggcgtcca cgccgtacgg cgccggcacc
     7201 ttgtaggcgt cggcgaactg gtggccgggc agcaggcgct cggcgttcgt gcagggggcg
     7261 gtggggatcg cgctcggtcg ggaggacatg cctgccactg tatgccccga atgccaactc
     7321 gaccggatgc tcctttcgcg accctgagcg ggaccgtgga ttgccgcccc gcgcgttcgt
     7381 cctcccgctt cccgcgttcg ccgccatgaa cctgcacgac tacgacgcct ggctcgcccc
     7441 gctcgcctcg gcccttggcg ccgccctcgt gggcatcctc gtccatgtca tgctgcgccc
     7501 gatcgtgcgg cgcctcgcgc gacatgcgcc cgtggccaac gcggtcgtgc agcagatcga
     7561 ccggccggtg cgctggctgc tgccgctgat ggccgtccag gtggtgctgg agggcgcgcc
     7621 cgacgacctg ccgcacatgg gcggcatgcg ccagctggtg acggtgctgc tgatcgccgc
     7681 gttcaccgcc accgccatcg ccgcggtgcg cggcgtcgcc gaggcggtcg cgctgctgca
     7741 tccgcaggac gtcgccgaca acctgcaggc gcgccgcatc ctcacgcaga cgcgcgtgct
     7801 cgcgcgcatc gcgatgggca tcgcgctgtt cgcgggcgtc gcgttcatcc tcatgacctt
     7861 cccgcgggca cgccagttcg gcaccagcct gctcgcgtcg gccggcctgt cggcgctcgt
     7921 gatcggcctg gccgcgaagt cggtgttcgg caacctgctc gcgggcctgc agatcgcgct
     7981 gtcgcagccg ctgcgcatcg acgacgtgct gatcgtgcag ggcgaatggg gccgcgtcga
     8041 ggagatcacc agcacctacg tcgtgctcaa gatctgggac gagcggcgcc tggtgatccc
     8101 gctgcagtgg ttcatcgaca acccgttcca gaactggacg cgcaccacct cgcagatcat
     8161 cggcagcgtc ttcttctggg tcgactacac caccgacctc gcgccgctgc gcgccgaggc
     8221 cacgcgcctt gcgcaaggtt ccaaggatca cgacggccgc gtctgcctgc tgcaggtggt
     8281 ggacacgagc gagcgcgcga tgaagctgcg gctgatcgtg agctcgccgt cggcgggcca
     8341 gagctgggac ctgtgctgcc tgctgcgcga gggcttggtc gccctgatgc agcgcgagca
     8401 tccggacgcg ttgccgcgcc tgcgcgccga gatgcgcgag gcggcccggc cgcacgacca
     8461 tcgcgacgag ctcgacgcgc gcatcgccgg ccagcgcacc gacccgggca gggcccaggt
     8521 caacaacgac accggactga ccagcctcac ggccccgacc ggcggggtgc tgccaccggc
     8581 cgaaccgccg cgttgagggc gctcgcgcac ggcgctgaca acggtgtcca aggctgatcg
     8641 atcgctccgc cccgcagtcg cttcggcctg ccggcctcac ccatgagcgg ttaagggtaa
     8701 ctactcgtta acggtgtgtc atcgcgaaaa ccaatgccac agaaacgaga tctgctcaac
     8761 agggagttcg tgaaccgttt cacgagttcg ggaaatcaac tacttttgcc ggacacttcc
     8821 tgacatcgtt tgtcctcgag ctgaacgaca atttgggctc cccctagttc cgtgcgtctc
     8881 cctccggcgc acccaacgac atgaaacgcc gcacgatgct gagcctgctg gcctccctgc
     8941 cggcagcacc ccttttcgcc aaggaatcgg gcaccctcgt cctcggccag tcggccccga
     9001 acacgggtcc gtcggcgcag ctgggcatcc agctgaaccg gggcgcgcgc ctgtacttcg
     9061 acaaggtcaa cgccgccggc ggcgtcaacg gccagaagat cgaactgcgc atgctggacg
     9121 acggctacga gccggcgcgc gcgaaggcca acaccgagaa gttcctcaac gacgacgtct
     9181 tcgccctgtt cggctacgtc ggcacgccca cctcggtggc ctgcctgccg atggtcaagg
     9241 acgccggcat cccgttcttc ggcccgttca ccggcgcgat ggcgctgcgc cagccggcgc
     9301 tgcgcaacgt gttccacgtg cgcgcgtcgt acgacgacga gacggcgctg atcgtcaacc
     9361 agctgcacaa cctgggcctc aagaagatcg ccgtcttcaa gcagaacgac gcctacggcc
     9421 aggccggcct cgacggcgtc aacaaggcgc tcaaggccct cgacctggcc
//
LOCUS       DATCRO010000564         9983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_107442, whole genome shotgun sequence.
ACCESSION   DATCRO010000564 DATCRO010000000
VERSION     DATCRO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107442"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VIN75_15525"
     CDS             complement(<1..765)
                     /locus_tag="VIN75_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8975628.1"
                     /translation="MPRPIRLPGRLAGPALAATALALAACAALPDAAAPPVPREPATI
                     SGARSFDAPTAAWPEQAWWHAYRDTQLDALIAEGLAGSPSIAMAQARLARAESLAQTS
                     RAADLPQLSANTSITQQRQSNDYLSPRAATPQGWNDYGRATLDLSWDLDFWGRNRAAL
                     RAALSDADAARADAAQARLMLAASIASAYAEFVRLCTAEDTAAEALEVRGKTVALFRQ
                     RRDNGLETLAAVRQVEARREMAEADLLSQREQVALQR"
     gene            complement(892..3741)
                     /locus_tag="VIN75_15530"
     CDS             complement(892..3741)
                     /locus_tag="VIN75_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8975629.1"
                     /translation="MATHDFLRLRFGSFEFDEADARLVVDGRPVALAPKAFGVLAALL
                     RQPGQLVTKGDLLDAVWGHRHVSESVLKTTISELRAALGDDAKRPRWVETASRRGYRF
                     IGLLEAEPLAGAPPAVADAPLVGRGAALERLRGAWSRAAGGQRQVCCVTGEAGIGKTS
                     LIDALVAGLGDPVVARGQCIEQAGAGEPYLPVLDALGSLVRRDPQVLPVLRTVAPTWL
                     LQMPWLVDAEEQQRLRAQLANTTQERMLREFAELVEQLSLVRPLLLVTEDLHWCDGAT
                     VSLLDHVARRRPPARLMWIASFRIAELVAEGHPLNGVRHELRARRLCDELLLDPFSES
                     ELQAYLEHRRPELARDERAVRRLHRQTDGLPLFVAVAVDDLGPHVPLARDATPDDWAV
                     SDTLAGVIERQLLRVPDGSRSLLEAAAVCGVEFETSVVAELMQADAADVAARCDELVR
                     RQYWLRPAPLETRADGSLEPRFAFRHALYRQVLYHRVGAPARAALHRRAAQALQRDPA
                     RHASAALALHHERGLEPLAALRAWVAVAETALGRFAPREALAITEHALEVLAHCPDGV
                     ERMDSELALMQVRGAACAHSIGIGTPQAREAFRRAAQLCELLPPTARRAWALSGLAWM
                     LMSRGEYALAEELAQRLLQAARQHGDRLLQATAAAILSAPNLQWGRLAAARSLVEEAI
                     TLTEGMEAVRLPNAVLLDPVAISRGIGAILYGHLGEVERARAAAAAAIDRAAAVGQPI
                     TRGFTWRCVCMVLVRLDEADAVLEIAGKLRQHVREHGLAQGEGPARTLLGWAQARHGD
                     ARAGHHELIQGMDDMRRLEAWFDLTMPLQLAADAARLAGDLDAAREHVEEGLALAERL
                     GERLHLPDLWLRRADLASTAGDVPRARTCAREALRVAEDMGARIAALAARLALCRLPG
                     ADAADLAALRACRQTWHDGEPFGVLRHVDALLAAS"
     gene            complement(3804..6434)
                     /locus_tag="VIN75_15535"
     CDS             complement(3804..6434)
                     /locus_tag="VIN75_15535"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY8975630.1"
                     /translation="MSALPSPPAQRASVAELRERFRSGKAALLEHFAGSRAAAPAATL
                     LLKSLARHVDQTLAELWQQAGMPSQAALLAVGGYGRGELFPYSDVDVLVLLPNAHMGG
                     DDPHSVALRNTIEGFITACWDIGLEIGSSVRTIDECLLEARADVTVQTALLESRFLTG
                     SRRMFNAFRRVNTEAMDPRAFLRAKTFEMRQRQVKYEDTPYSLEPNCKESPGGLRDLQ
                     VLTWIARAAGLGRSWNEMAAHGLLTAFEVKRLQRHEGTLKLIRARLHLVAKRREDRLV
                     FDLQTAVAESFGYQGSTTMRASEMLMQRYYWAAKAVSQLNEVLLLNLEERINGSEDLP
                     MQPLNERFLDRGGLLEVISDDLYKREPHAILETFHLYQTTPHLRGLSARTMRALYNVR
                     NQMNGAFRKDPVNRARFLAILQAPEGQTHAFRLMNATSVLGRYLWVFRRIVGRMQHDL
                     FHVYTVDQHILMVLRNVRRFFIPEHAHEYPKCTALAASFDKPWLLYIAALFHDVAKGR
                     GGDHSELGGAEVRHFCRDHGIAREDADFIEFLVRQHLTMSNVAQKEDLSDAAVIADFA
                     AKVGNPRRLTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRLTLRKLGGEVLALDAEI
                     EARKKEARQILALHSALPGTEAPLWKTLDLGYFARHDPNELAWHARSLWRHLETAAPV
                     VRARPSPHGEGLQVLLFSPDRADLFARICGYFDSAGFSIQDAKIHTTNAGFALDTFQV
                     INPAYEDDGPAGYRDLIPLVEHQAALAAGSEGPLPTPRASRVSRRVKSFPVVPRVSLR
                     PDDRAQRWLLSISASDRSGLLYAVARVLAQYRINLQLAKVSTLGERVEDTFLVDGAAL
                     QNNRLQIQVETELLDAISAT"
     gene            complement(6449..7300)
                     /gene="map"
                     /locus_tag="VIN75_15540"
     CDS             complement(6449..7300)
                     /gene="map"
                     /locus_tag="VIN75_15540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8975631.1"
                     /translation="MTITLKSASDIEGMRIAGRLGSEVLDMLTPHVKPGVTTEQLDKL
                     AHDYIFDVQKGIPAPLNYCPPGYTPYPKSICTSINHQVCHGIPNDRPLKDGDIVNIDV
                     TVIKDGWHGDTSRMFVVGKGSIAARRLCQITYEAMWKGIEKVKPGARLGDIGHAIQVF
                     AENAGYSVVREFCGHGIGQKFHEEPQVLHYGRPGTLEELVPGMIFTIEPMINAGKRDI
                     KEFGDGWTIVTKDRSLSAQWEHTIVVTETGYEVLTVSAGSPAVPDFVKNPVLNRLLVD
                     APSGVAI"
     gene            7598..>9983
                     /gene="purL"
                     /locus_tag="VIN75_15545"
                     /gene_synonym="purI"
     CDS             7598..>9983
                     /gene="purL"
                     /locus_tag="VIN75_15545"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY8975632.1"
                     /translation="MSSPTQKHLLHFEGGNALSAFRAQALLPKLQSVSPRITGVAARH
                     VHWVWSDEAVQPDAMRKLASLLRYGDAYDGPGDDAADSVLVVVMPRLGTVSPWASKAT
                     DIAHNCGLALHRVERVTEFRLTLKTGLLGGAKALSEQELQAAAMLLHDRMTESVAFER
                     EAAVHLFDKQPAEPLVHVDLLGQGRDALVVANAQFGLALSDDEIDYLVDTFKGLDRNR
                     TDVELMMFAQANSEHCRHKIFNAKFIIDGQAQDISMFGMIRNTEKLNSQHSIVAYSDN
                     AAVMEGHAIERWVAAPMKGDGPAQYAGRAETAHVLMKVETHNHPTAISPFPGASTGSG
                     GEIRDEGATGRGSKPKAGLAGFSVGNLYLPGTSESWERSPVGKPEHIASALQIMIDGP
                     LGAAAFNNEFGRPNLAGYFRVFEQDVAGVTRGYHKPIMIAGGLGAIRDDLTHKIEFPA
                     GTLLVQLGGPGMRIGMGGGAASSMAAGTNTAALDFDSVQRGNPEIQRRAQEVINQCWQ
                     LGAANPILAIHDVGAGGLSNAFPELAEGAGTGAVFDLRAVPLEESGLAPKEIWCNESQ
                     ERYTLAIAPESLPLLKSFAARERCPFAVVGRATERKSLVLEDGAGGERAIDMPMEVLF
                     GKPPKLLRDVTRVERVETPLDLSGVKLEDAALDVLRHPTVASKRFLITIGDRTVGGLS
                     HRDQMVGPWQVPVADVAVTLADFKGFAGEAMSMGERTPLASVHAPASGRMAVAEAITN
                     LLAAPIELARVKLSCNWMAACGEPGEDAALYDTVRAVGMELCPALGISVPVGKDS"
BASE COUNT         1445 a         3465 c         3663 g         1410 t
ORIGIN      
        1 gcgctgcagc gcgacctgct cgcgctgcga cagcaggtcg gcctcggcca tctcgcgccg
       61 cgcctcgacc tggcgcacgg cggccagggt ctccaggccg ttgtcgcggc gctggcggaa
      121 cagcgccacg gtcttcccgc gcacctcgag cgcctcggcg gcggtgtctt cggcggtgca
      181 caggcgcacg aattcggcat aggccgacgc gatggacgcc gccagcatca gccgagcctg
      241 cgccgcgtcg gcgcgcgcgg cgtcggcatc cgacagcgcc gcccgcaacg cggcgcggtt
      301 gcggccccag aagtccaggt cccaggacag atcgagcgtc gcgcgtccat agtcgttcca
      361 gccctggggc gtggcggcgc gcgggctgag gtagtcgttg ctctgcctct gctgcgtgat
      421 cgacgtgttg gccgacagct ggggcaggtc ggcggcgcgt gacgtctgcg ccagcgattc
      481 ggcgcgtgcg aggcgggcct gggccatggc gatcgagggc gagccggcga gtccttcggc
      541 gatgagcgcg tcgagctgcg tgtcgcgata ggcgtgccac caggcctgtt cgggccaggc
      601 ggcggtgggc gcatcgaagc tgcgcgcccc ggagatcgtg gcgggctcgc gtggaaccgg
      661 cggggcggcg gcgtcgggca aggccgcgca ggcggccagg gccagcgcgg tggcggcgag
      721 cgccggaccg gccagtcggc cgggcaatcg gatggggcgg ggcatcgtcg gtttcctctg
      781 tgctcggacg gcgcggcacg cgccttttcc atgaccgaga ggctaaggac gatgacgcgg
      841 gaagtcgttc gggaacggtc gggaaacggt caggatcgtg gccggacgga ctcacgaggc
      901 ggcaagcagc gcgtcgacat gccgcagcac gccgaagggc tcgccgtcat gccacgtctg
      961 gcgacaggcg cgcagggcgg ccagatccgc cgcgtcggcg ccgggcagtc ggcacagtgc
     1021 caggcgcgcg gccagggcgg cgatgcgcgc gcccatgtcc tcggcgacgc gcagcgcctc
     1081 gcgcgcgcag gtgcgggcgc gcgggacatc gcccgcggtg ctcgcaaggt ccgcacggcg
     1141 cagccacagg tcgggcaggt gcagccgctc gcccaggcgc tcggccaggg cgaggccttc
     1201 ctcgacatgc tcgcgggcgg cgtccaggtc gccggccagc cgcgccgcat cggccgcgag
     1261 ctgcaggggc atcgtgagat cgaaccaggc ttcgagccgg cgcatgtcgt ccatgccctg
     1321 gatcagctca tggtgacccg cccgcgcatc gccgtgccgg gcctgcgccc atcccagcag
     1381 cgtcctcgcc ggaccttcgc cctgtgcgag gccgtgctcg cgcacgtgct ggcgcagctt
     1441 gccggcgatc tcgagcacgg cgtcggcctc gtccagccgc accagcacca tgcagacgca
     1501 gcgccaggtg aagccgcgcg tgatcggctg gccgaccgcc gcggcgcggt cgatcgccgc
     1561 ggccgcggcg gcgcgggcgc gctcgacctc tcccaggtgc ccatacagga tcgcgccgat
     1621 gccgcgtgat atcgccaccg gatccagcag gacggcgttg ggcaggcgaa cggcctccat
     1681 gccttcggtc agcgtgatcg cttcctcgac cagcgaccgc gccgccgcca ggcgtcccca
     1741 ttgcaggttc ggtgcggaca ggatggccgc ggccgtggct tgcagcaggc ggtcgccatg
     1801 ctggcgcgcg gcctgcagca gccgctgcgc cagttcctcg gccagcgcgt actcgccgcg
     1861 tgacatcagc atccaggcca ggccgctgag cgcccaggcg cggcgcgccg tgggcggcag
     1921 gagctcgcac agctgcgcgg cgcgccggaa ggcctcgcgg gcctggggcg tgccgatgcc
     1981 gatggagtgc gcgcaggcgg cgccgcgcac ctgcatgagc gcgagttcgg agtccatgcg
     2041 ttcgacgccg tccgggcagt gcgccaggac ttcgagcgcg tgctcggtga tcgccagtgc
     2101 ctcgcgcggc gcgaagcggc ccagtgccgt ctcggcgacg gcgacccagg cgcgcagggc
     2161 ggccagaggc tcgagcccgc gctcgtggtg cagggcgagc gctgcggaag cgtggcgggc
     2221 agggtcgcgc tgcagcgcct gtgcggcgcg ccgatggagc gccgcgcgcg ccggggcgcc
     2281 gacgcggtgg tacagcacct ggcgatacag cgcatggcgg aacgcgaagc gtggttccag
     2341 ggacccgtcg gcgcgggtct cgagcggcgc cgggcgcagc cagtactggc gccgcacgag
     2401 ctcgtcgcag cgggccgcga cgtccgccgc gtcggcctgc atcaactccg ccacgacgga
     2461 ggtttcgaac tccacgccgc atacggcggc tgcctccagc aacgagcgcg agccgtccgg
     2521 cacgcgcagc agctggcgtt cgatgacgcc ggccagggtg tcggacacgg cccagtcgtc
     2581 tggcgtggca tcgcgcgcca gcgggacgtg ggggccgagg tcgtcgaccg ccacggccac
     2641 gaacagcggc aggccatcgg tctgccggtg caggcgccgc acggcgcgtt cgtcgcgtgc
     2701 cagctcgggt cgccggtgtt cgagataggc ctggagctcg gactcgctga atgggtcgag
     2761 cagcagttcg tcgcacagcc gccgggcgcg caactcgtgg cgcacgccgt tgagcggatg
     2821 cccttccgcc accagctcgg cgatgcggaa gctggcgatc cacatcagcc gggccggcgg
     2881 ccgccggcgg gcgacgtgat cgagcaggct caccgtcgcg ccgtcgcacc agtgcaggtc
     2941 ctccgtgacc agcagcagcg gccgcacgag gctcagctgc tcgacgagct cggcgaactc
     3001 gcgcagcatg cgctcctgcg tcgtgttggc caactgcgcg cgcaggcgct gctgttcctc
     3061 ggcgtccacg agccagggca tctgcagcag ccaggtcggc gcgaccgttc gcaggaccgg
     3121 cagcacctgc gggtcgcggc gcacgaggct gcccagcgcg tcaagtacgg gcaagtaggg
     3181 ctcgcccgcg ccggcttgct cgatgcactg gccgcgcgcg acgaccgggt cgcccaggcc
     3241 ggcgacgagg gcgtcgatca gcgaggtctt gccgatgccg gcctcgcccg tgacgcagca
     3301 cacctggcgc tggcctcccg ctgcgcgtga ccaggccccc cgcagacgct cgagcgccgc
     3361 gccgcggccg accagcgggg catcggcgac ggccggcggg gccccggcaa gcggttccgc
     3421 ctcgagaagg ccgatgaagc gatagccgcg gcgcgaggcc gtctcgaccc agcgcggtcg
     3481 cttggcgtcg tcgcccagcg cggcgcgcag ctcgctgatg gtggtcttga gcaccgactc
     3541 gctcacatgg cgatggcccc agacggcatc gagcaggtcg cccttggtca cgagctgccc
     3601 gggctggcgc agcaacgccg ccagtacgcc gaaggccttg ggcgcgagag ccaccggccg
     3661 gccgtccacg accagccgcg cgtcggcctc gtcgaactcg aacgaaccga agcgcagccg
     3721 caggaaatcg tgcgtggcca tgtcccgaaa ttatgggctc gccggacggc tgcccgggcg
     3781 agatcggcgc ttcaagcgcg ggatcaggtc gcgctgatcg cgtccagcag ctccgtctcc
     3841 acctggatct gcagccggtt gttctgcagc gcggcgccgt ccacgaggaa ggtgtcctcg
     3901 acgcgctcgc cgagcgtcga gaccttggcc agctgcaggt tgatgcggta ctgcgccagc
     3961 acgcgcgcca ccgcgtacag caggccggag cggtcgctgg cgctgatgct cagcagccag
     4021 cgctgggcac ggtcgtccgg acgcagcgac acgcgcggca ccaccggaaa cgacttgacg
     4081 cggcgcgaca cgcggctggc gcgcggcgtg ggcagcgggc cctcggagcc ggcggccagc
     4141 gcggcctggt gctccaccag cgggatcagg tcgcggtagc cggcggggcc gtcgtcctcg
     4201 taggccgggt tgatgacctg gaaggtgtcc agcgcgaagc cggcgttggt ggtgtggatc
     4261 ttggcgtcct ggatggagaa cccggcgctg tcgaagtagc cgcagatgcg ggcgaacagg
     4321 tcggcgcggt cgggcgagaa cagcagcacc tgcaggccct cgccgtgcgg cgacggacgc
     4381 gcgcgcacga cgggcgccgc cgtctccagg tggcgccaca gcgagcgcgc gtgccaggcc
     4441 agctcgttgg ggtcgtggcg cgcgaagtag ccgaggtcca gcgtcttcca cagcggcgcc
     4501 tcggtgccgg gcagcgcgga gtgcagcgcg aggatctggc gcgcctcctt cttgcgcgcc
     4561 tcgatctcgg cgtcgagcgc cagcacctcg ccgccgagct tgcgcagcgt caggcgatag
     4621 aggtcctcga gcagcttgcc cttccacgcg ttccagacct tgggactggt gccgcggatg
     4681 tcggccacgg tcagcaggta cagggcggtc aggcggcgcg ggttgccgac cttggcggcg
     4741 aagtcggcga tgacggccgc gtcggacagg tcctccttct gcgccacgtt gctcatggtc
     4801 aggtgctggc gcacgaggaa ctcgatgaag tcggcgtcct ccctggcgat gccgtggtcg
     4861 cggcagaagt ggcgcacctc ggcgcccccg agctccgaat ggtcgccgcc gcggcccttg
     4921 gccacgtcat ggaacagcgc ggcgatgtac agcagccacg gcttgtcgaa gctcgcggcc
     4981 agcgcggtgc acttggggta ctcgtgcgcg tgctcgggga tgaagaagcg gcgcacgttg
     5041 cgcagcacca tcaggatgtg ctggtcgacc gtgtagacgt ggaacaggtc gtgctgcatc
     5101 cggccgacga tgcggcggaa cacccacagg tagcgcccca gcaccgacgt ggcgttcatc
     5161 agccggaacg cgtgcgtctg gccttcgggc gcctggagga tggccaggaa gcgcgcgcgg
     5221 ttgaccggat ccttgcggaa cgcgccgttc atctggtttc gcacgttgta cagcgcacgc
     5281 atcgtgcgcg ccgacaggcc gcgcaggtgc ggcgtggtct ggtacaggtg gaaggtctcg
     5341 aggatcgcgt gcggctcgcg cttgtacagg tcgtcggaga tcacctccag caggccgccg
     5401 cgatcgagga agcgctcgtt gagcggctgc atcggcaggt cttccgagcc gttgatgcgc
     5461 tcctcgaggt tcagcagcag cacctcgttc aactgcgaga ccgccttggc cgcccagtag
     5521 tagcgctgca tcagcatctc ggaggcgcgc atcgtggtgc tgccctggta gccgaaggac
     5581 tcggccacgg cggtctgcag gtcgaacacg aggcgatcct cgcgccgctt ggccaccagg
     5641 tgcagccgcg cgcggatcag cttgagcgtg ccttcgtggc gctgcaggcg cttgacctcg
     5701 aacgcggtca gcaggccgtg cgcggccatc tcgttccagc tgcggcccag gcccgcggcg
     5761 cgcgcgatcc aggtcagcac ctgcaggtcg cgcaggccgc ccgggctttc cttgcagttg
     5821 ggctccagcg agtacggcgt gtcctcgtac ttgacctggc gctggcgcat ctcgaaggtc
     5881 ttggcgcgca ggaaggcgcg cgggtccatc gcctcggtgt tgacgcgccg gaacgcgttg
     5941 aacatgcggc gcgagccggt caggaagcgg ctctcgagca gcgcggtctg cacggtgacg
     6001 tcggcgcgcg cctcgagcag gcactcgtcg atggtgcgca ccgacgagcc gatctccagg
     6061 ccgatgtccc agcacgcggt gatgaagccc tcgatcgtgt tgcgcagcgc caccgaatgc
     6121 gggtcgtcgc cgcccatgtg cgcgttgggc agcagcacca ggacgtcgac gtcggaatac
     6181 gggaacagct cgccgcggcc gtagccgccg acggccagca gcgcggcctg cgaaggcatg
     6241 ccggcctgct gccacagctc ggccagcgtc tggtcgacgt gcctggccag cgacttgagc
     6301 agcaaggtgg cggccggcgc ggcggcgcgc gacccggcga aatgttcgag cagggcggcc
     6361 ttgcccgagc ggaaacgctc gcgcaactcg gccacggagg cgcgctgcgc gggcggcgag
     6421 ggcagggcgg acaccgaggt cggaaccgtc agatcgcgac gccgctgggg gcgtcgacga
     6481 gcagccggtt gagcaccggg ttcttcacga agtcgggcac ggccgggctg ccggcggaca
     6541 ccgtcagcac ctcgtagccc gtctcggtga cgacgatcgt gtgctcccac tgggccgaca
     6601 gcgagcggtc cttggtgacg atggtccagc cgtcgccgaa ttccttgatg tcgcgcttgc
     6661 cggcgttgat catcggctcg atcgtgaaga tcatgccggg caccagctcc tcgagcgtgc
     6721 cggggcggcc gtagtgcagc acctggggct cctcgtggaa cttctgcccg atgccgtggc
     6781 cgcagaactc gcgcaccacc gagtagccgg cgttctcggc gaacacctgg atggcatggc
     6841 cgatgtcgcc caggcgcgcg cccggcttga ccttctcgat gcccttccac atcgcctcgt
     6901 aggtgatctg gcacagccgc ctggccgcga tggagccctt gccgaccacg aacatgcggc
     6961 tcgtgtcgcc gtgccagccg tccttgatga cggtgacgtc gatgttgacg atgtcgccgt
     7021 ccttgagcgg tcggtcgttg gggatgccgt ggcagacctg gtggttgatc gacgtgcaga
     7081 tcgacttcgg gtagggcgtg tagcccggcg ggcagtagtt cagcggggcc gggatgccct
     7141 tctggacgtc gaagatgtag tcgtgggcca gcttgtcgag ctgttcggtg gtgacaccag
     7201 gcttgacatg cggggtgagc atgtccagca cttccgagcc cagccggccg gcgatgcgca
     7261 tgccctcgat gtcggaggcg gacttcaggg tgatcgtcat ggttcccgaa ttatcccatc
     7321 ggagggagtg acttggctgg cggcgctgtc ttgtcgcgtt tgacgcattt cgggcggatc
     7381 cgcctggggg aagcacgacg gatcggggga gtcttcgcgg cgaggtcaag gaggaggagg
     7441 ccggtggaca tgaacaagaa tgactccgcc ggggcgtcgt gccttccccc ggaactgccc
     7501 gagcacacca ccctccgcta aaatcaggga ttctgcttag ttcgcccccg ggatggaccg
     7561 agcaacccca acagacacct ccgcgagccc acaccgcatg tcgtccccca cgcaaaagca
     7621 cctgctccac ttcgagggcg gcaacgccct gtccgccttc cgcgcccagg cgctgttgcc
     7681 gaagctgcag tccgtctcgc cgcgcatcac gggggtcgcg gcgcggcacg tgcactgggt
     7741 ctggtcggac gaggccgtcc agcccgatgc catgcgcaag ctggccagcc tgctgcgcta
     7801 cggcgatgcc tacgacggtc cgggcgacga tgccgccgac agcgtgctgg tcgtggtgat
     7861 gccgcggctg ggcaccgtgt cgccctgggc cagcaaggcc accgacatcg cccacaactg
     7921 cggcctggcg ctgcaccgcg tcgagcgcgt caccgaattc cgcctgacgc tcaagaccgg
     7981 cctgctgggc ggcgccaagg cgctgtccga acaggaattg caggccgcgg ccatgctgct
     8041 gcacgaccgc atgaccgaga gcgtcgcgtt cgagcgcgag gccgccgtcc acctgttcga
     8101 caagcagccg gccgaaccgc tggtgcacgt cgacctgctg gggcagggcc gcgatgcgct
     8161 ggtcgtggcc aacgcgcaat tcggcctggc gttgtcggac gacgagatcg attacctcgt
     8221 cgacaccttc aagggcctgg atcgcaaccg gaccgacgtc gagctcatga tgttcgcgca
     8281 ggccaacagc gaacactgcc gccacaagat cttcaacgcg aagttcatca tcgacggcca
     8341 ggcccaggac atctcgatgt tcggcatgat ccgcaacacc gagaagctca attcgcagca
     8401 ctcgatcgtc gcgtactccg acaacgcggc cgtcatggaa ggccacgcga tcgagcgctg
     8461 ggtcgcggcg ccgatgaagg gcgacggccc cgcgcagtac gccggccgcg ccgagaccgc
     8521 gcacgtgctg atgaaggtcg agacgcacaa ccacccgacc gccatctcgc cgttccccgg
     8581 cgcgtcgacc ggctccggcg gcgagatccg cgacgagggc gcgaccggcc gcggctccaa
     8641 gcccaaggcc ggcctggccg gcttctcggt gggcaacctg tacctgcccg gcaccagcga
     8701 gtcgtgggag cgctcgccgg tgggcaagcc cgagcacatc gccagcgcgc tgcagatcat
     8761 gatcgacggc ccgctcggcg ccgcggcgtt caacaacgag ttcggccgcc ccaacctggc
     8821 cggctacttc cgcgtgttcg agcaggacgt cgccggcgtc acgcgcggct accacaagcc
     8881 catcatgatc gcgggcggcc tgggcgcgat ccgcgacgac ctgacccaca agatcgagtt
     8941 cccggccggc acgctgctgg tgcaactggg cggcccgggc atgcgcatcg gcatgggcgg
     9001 cggcgccgcc agctcgatgg cggccggcac caacaccgcg gcgctggact tcgactccgt
     9061 gcagcgcggc aaccccgaga tccagcgccg cgcgcaggaa gtcatcaacc agtgctggca
     9121 gctgggcgcg gccaacccca tcctggcgat ccacgacgtg ggcgcgggcg gcctgtccaa
     9181 cgcgttcccc gagctggccg aaggcgcggg caccggcgcc gtgttcgacc tgcgcgccgt
     9241 gccgctggag gaatccggcc tcgcgcccaa ggagatctgg tgcaacgaga gccaggagcg
     9301 ctacacgctg gccatcgctc ccgagtcgct gccgctgctc aagtcgttcg ccgcgcgcga
     9361 acgctgcccg ttcgcggtgg tcggacgcgc gaccgagcgc aagtcgctgg tgctggaaga
     9421 cggcgccggc ggcgaacgcg ccatcgacat gccgatggag gtgctgttcg gcaagccgcc
     9481 caagctgctg cgcgacgtca cgcgcgtcga gcgcgtcgag acgcccctcg acctgtccgg
     9541 cgtgaagctg gaggacgccg cgctcgacgt gctgcgccat ccgaccgtgg cctccaagcg
     9601 cttcctgatc accatcggcg accgcacggt gggcggcctg tcccaccggg accagatggt
     9661 cggcccgtgg caggtgccgg tggccgacgt ggccgtgacg ctggccgatt tcaagggctt
     9721 tgccggtgag gcgatgagca tgggcgagcg cacgccgctc gcgtccgtgc acgcgccggc
     9781 ctcgggccgc atggccgtgg ccgaggccat caccaacctg ctggccgcgc cgatcgagct
     9841 ggcgcgcgtc aagctgtcgt gcaactggat ggccgcgtgc ggcgagccgg gcgaggacgc
     9901 ggcgctgtac gacacggtgc gcgcggtggg catggagctg tgcccggcgc tgggcatcag
     9961 cgtgccggtg ggcaaggaca gcc
//
LOCUS       DATCRO010000565         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1052444, whole genome shotgun sequence.
ACCESSION   DATCRO010000565 DATCRO010000000
VERSION     DATCRO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052444"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VIN75_15550"
     CDS             <1..926
                     /locus_tag="VIN75_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8975633.1"
                     /translation="LILSNDGALRIPAVPKTLGVIGSGVIGLEMGSVWRRLGAQVTIL
                     EALPTFLGAADESVAKEAAKAFAKQNLKIELGVKVTKVDSSGKGVSVSYTDAKGAEQV
                     LAVDRLIVSIGRVANTIGLNPEAVGLKLDERGMVVVDDECRTNLPGVWAIGDVVRGPM
                     LAHKAEEEGVAVAERIAGQHGHVNFNTVPWVIYTSPEIAWVGQTEQQLKTEGRAYKAG
                     QFPFLANGRARALGDTTGFVKFIADAKTDEILGVHMIGPMVSELISEACVAMEFKASA
                     EDIARICHAHPSLSEATKEAALAVDKRTLNF"
     gene            1093..2193
                     /gene="zapE"
                     /locus_tag="VIN75_15555"
     CDS             1093..2193
                     /gene="zapE"
                     /locus_tag="VIN75_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY8975634.1"
                     /translation="MPTVTELYHQTLAERGFRADAAQLNAVAALQRCEDEWIAYKSRR
                     SNAITKMLVRPPIPRGVYMYGGVGRGKSFLMDCFFQSVPLKRKVRLHFHEFMREVHRE
                     LQDLKGVADPLQELGRRISLRYRLICFDEFHIADVTDAMILYRLLLSLFDNRVSFVTT
                     SNFRPDDLYPNGLNRDRILPAIALLNDKLEVINVDAGTDYRRRTLTDAKLYHTPLGPE
                     ADADMAATFDQIAEARDEDPLLHIEHREIRARRRAGGVVWFDFKELCGGPRSMNDYLE
                     LATQFHTLMLSDVPQMSPRLASEARRFTWLVDVLYDRRVKLVISAEVPPEQLYLDGPL
                     AHEFPRTISRLQEMQSQEYLAISKRDVDTGLT"
     gene            complement(2259..3083)
                     /locus_tag="VIN75_15560"
     CDS             complement(2259..3083)
                     /locus_tag="VIN75_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HEY8975635.1"
                     /translation="MDATNTLPPLLGLALVALVLGMRHGVDADHLAAIDAMTRCNAQG
                     RPALARRTGVWFSVGHGAIVMAVALAVAATTAAWNVPAWLAPFGAWTSIAVLVLLGAL
                     NLAAWQRAAPGAPVALAGWRSRLFAGALRACTRRAVMGVGMLFALSFDTLTQAALMAA
                     TGVARHGLPTVALLAGAFAAGMVLTDGVNGWWVARLVQRPGAGSARASRLMCIAVGAI
                     SLGTAAIGAAAQLSGPIAGWAQAHGEWVAGAIVAIMACSFGAAALLARRDASVATA"
     gene            3362..>3801
                     /locus_tag="VIN75_15565"
     CDS             3362..>3801
                     /locus_tag="VIN75_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8975636.1"
                     /translation="METSRQGDVLFLLLGAVMVLAMHAGFAFLELGTVRKKNQVNALV
                     KILVDFSVSTVAYFFIGYMVAYGTSFFVDARHLALHSGYELVRFFFLMTFAAAVPAIV
                     SGGIAERARFEPQLAATAVIVGLAYPLLEGAAWNDRFGIQGVLKG"
BASE COUNT          598 a         1341 c         1317 g          545 t
ORIGIN      
        1 acctcatcct ttccaacgac ggtgccctgc gcattccggc tgtgccgaag acgctgggcg
       61 tcatcggttc gggcgttatc ggccttgaga tgggttcggt gtggcgacga cttggcgccc
      121 aggtcaccat cctggaagcg ctgcccacgt tcctgggcgc ggccgacgag tcggtcgcca
      181 aggaagcggc caaggccttc gccaagcaga acctcaagat cgagctgggc gtgaaagtca
      241 ccaaggtcga ctccagcggc aagggcgtgt cggtgtcgta cacggacgcc aagggcgccg
      301 agcaggtgct ggccgtcgac cggctgatcg tctcgatcgg ccgcgtcgcc aacaccatcg
      361 gcctgaatcc cgaggccgtg ggcctgaagc tggacgagcg cggcatggtc gtggtcgacg
      421 acgagtgccg cacgaacctg ccgggcgtct gggccatcgg cgacgtcgtg cgcggcccga
      481 tgctggcgca caaggccgag gaagagggcg tcgcggtggc cgagcgcatc gcgggccagc
      541 acggccacgt caacttcaac acggtgccct gggtcatcta cacgagcccc gagatcgcgt
      601 gggtgggcca gaccgagcag cagctcaaga ccgaaggccg cgcctacaag gccggccagt
      661 tcccgttcct cgccaacggc cgcgcgcgcg cgctgggcga caccacgggc ttcgtcaagt
      721 tcatcgccga cgcgaagacc gacgagatcc tgggcgtgca catgatcggc ccgatggtct
      781 cggagctgat ctcggaagcc tgcgtggcga tggagttcaa ggcctcggcc gaggacatcg
      841 cgcgcatctg ccatgcgcac ccgtcgctgt cggaagccac caaggaagcc gcgctggccg
      901 tcgacaagcg cactctgaac ttctgatcgc aggggtctgg cctatcctcg tgccgccgca
      961 ggcggggcga ggccaggcct gaccccgctc gcgctgaaat cgtccccggg ggatcggggt
     1021 caggcctgtc cgcgaatcgc tgcgcgattc gtgcagagtc cagacccctc cggccccgcc
     1081 gctcgcccgt ccatgcccac cgtcaccgag ctgtaccacc agaccctcgc cgagcgcggc
     1141 ttccgggccg atgccgcgca gctgaacgcc gtcgcggcgc tgcagcgttg cgaggacgag
     1201 tggatcgcgt acaagtcgcg ccgctccaac gcgatcacca agatgctggt gcgcccgccg
     1261 atcccgcgcg gcgtctacat gtacggcggc gtcgggcgcg gcaagagctt cctgatggac
     1321 tgcttcttcc agtccgtgcc gctcaagcgc aaggtgcggc tgcacttcca cgagttcatg
     1381 cgcgaggtgc accgcgagct gcaggacctg aagggcgtcg ccgacccgct gcaggaactg
     1441 gggcgccgca tctcgctgcg ctaccggctg atctgcttcg acgagttcca catcgccgac
     1501 gtcaccgacg cgatgatcct gtaccggctg ctgctgtcgc tgttcgacaa ccgcgtctcg
     1561 ttcgtcacga cctcgaactt ccggcccgac gacctgtacc cgaatggcct gaaccgcgac
     1621 cgcatcctgc ctgcgatcgc gctgctcaac gacaagctcg aggtgatcaa cgtcgacgcc
     1681 ggcaccgact accgccggcg cacgctcacc gacgccaagc tgtaccacac gccgctcggg
     1741 cccgaggcgg acgccgacat ggcggccacc ttcgaccaga tcgccgaggc gcgcgacgag
     1801 gatccgctgc tgcacatcga gcaccgcgag atccgcgccc ggcggcgcgc cggcggcgtc
     1861 gtgtggttcg acttcaagga attgtgcggc ggcccgcgct cgatgaacga ctacctcgag
     1921 ctcgcgaccc agttccacac gctgatgctg tcggacgtgc cgcagatgtc gccgcgcctg
     1981 gcctccgagg cgcgccgctt cacgtggctg gtggacgtgc tgtacgaccg ccgcgtcaag
     2041 ctggtcatct ccgccgaggt gccgcccgag cagctgtacc tggacggccc gctggcgcac
     2101 gagttcccgc gcacgatctc gcggctgcag gagatgcagt cgcaggaata cctggcgatc
     2161 tccaagcggg acgtcgacac cgggctgacc tgagaagccg tggacgaatc ccgcacgccc
     2221 gagtcggcgt gcacgtgacg tgcgaccgcg caccgagctc aggccgtcgc gacggacgcg
     2281 tcccggcgcg ccagcagcgc cgcggcgccg aagctgcacg ccatgatggc gacgatcgcg
     2341 ccggccaccc actcgccgtg cgcctgcgcc cagcccgcga tcggccccga cagctgcgcc
     2401 gccgcgccga tcgccgccgt gcccaggctg atcgcgccga ccgcgatgca catcaggcgc
     2461 gaggcccgcg ccgagccggc gccgggacgc tgcaccaggc gcgcgaccca ccagccgttg
     2521 acgccgtcgg tcaggaccat gccggcggcg aacgcgccgg ccagcagcgc cacggtgggc
     2581 aagccatgcc gcgcgacgcc cgtggccgcc atcagggcag cctgggtgag cgtgtcgaac
     2641 gacagcgcga acagcatgcc cacgcccatc acggcgcgcc gcgtgcaggc gcgcagcgca
     2701 ccggcgaaca gccggctgcg ccagccggcg agggcgacgg gtgcgccggg ggccgcgcgc
     2761 tgccaggctg ccaggttgag cgcgcccagg agcaccagca cggcgatcga cgtccaggcc
     2821 ccgaacggtg ccagccacgc cgggacgttc caggcggccg tcgtcgcggc caccgccagc
     2881 gccacggcca tcacgatggc gccgtggccg accgagaacc acacgcccgt gcgacgcgcc
     2941 agcgccgggc ggccctgggc gttgcagcgc gtcatcgcgt cgatggcggc caggtggtcg
     3001 gcatcgacgc catggcgcat gcccagcacc agcgcgacga gcgcgaggcc gagcaggggc
     3061 gggagcgtgt tggtggcgtc catggggctc ggacgactat agacccgggc cgcggcggcg
     3121 gggcggtcgc gccgcgggac cgttggtggg aggcccttgg caggcggtgg cgttcctaaa
     3181 cggggcgaca tgcattgaaa tggggcgcaa tgcactgcaa cggtgttcag cttggattca
     3241 aggccccgcg gcatggctcg ggagccgctt catttgtgag catgaatatt gcgtcatggt
     3301 gcgccggcgt cgaaacctgc accatcgtgg aatgcggggc gccccttcgt gaaagaagaa
     3361 gatggagacc tcgagacaag gcgacgtcct gttcctgctg ctgggcgccg tgatggtgct
     3421 ggcgatgcac gccggcttcg cgttcctgga gctgggcacc gtgcgcaaga agaaccaggt
     3481 caacgcgctc gtgaagatcc tcgtggactt ctcggtgtcg accgtggcgt acttcttcat
     3541 cggctacatg gtggcgtacg gcacgtcgtt cttcgtcgac gcccggcacc tggcgctgca
     3601 cagcggctac gagctggtgc gcttcttctt cctgatgacg ttcgcggccg cggtgccggc
     3661 catcgtgtcg ggcggcatcg ccgagcgcgc gcgcttcgag ccgcagctgg ccgccacggc
     3721 cgtcatcgtg ggcctggcct atccgctgct ggagggcgcg gcatggaacg accgcttcgg
     3781 catccagggc gtgctcaagg g
//
LOCUS       DATCRO010000566         8985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_141574, whole genome shotgun sequence.
ACCESSION   DATCRO010000566 DATCRO010000000
VERSION     DATCRO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141574"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..685)
                     /locus_tag="VIN75_15570"
     CDS             complement(83..685)
                     /locus_tag="VIN75_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975637.1"
                     /translation="MTAPLRSFLHIAIAAALTVAGAAAHAQAKAAAPAAAPANLPAPD
                     AEKQKAIDRILAVFHPENGVLQAVQRQGVDAMQQSNIALHTAHVPKERADKTLKEIGV
                     DVQKYIDTTMPIAVASAKKNTNPAVGPILAQNFTADELRQIAALLESPLKARFEKLVP
                     QMEDAVGHKVQADISPQVNANVKTMTEAVSTKLQIAATAQ"
     gene            complement(705..2375)
                     /locus_tag="VIN75_15575"
     CDS             complement(705..2375)
                     /locus_tag="VIN75_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY8975638.1"
                     /translation="MTVPPTTRPARLRPVSRQTPPARTILSTCIAAGALLSPQAHAAL
                     TENLAVSPVAMSLGNAVTADPPGLDSIHFNPAGLAKITSDTRSDTIFGAALRTTAQFH
                     QPAGFDVGGFTEDPLNGTHSDHNRQAIFIPIAGVPSPRLPFAAAAGLALSWHNEGSPW
                     TFATGTYVPQAVGIDRTKNPDDPARFDGKQVVIQRLVYLSPSVGYKVNDQFQVGLAIP
                     IAHQGFALDTEMRFPNKLLGIIGKLQDAWCGPSGNPLDELGFGLCGTDSTGKQSGRLR
                     PFDSIGEMRFSGTAPADVTFNVGALWQPSDKLGFGAVYQSGSKTVITGDYNFHANPML
                     PEFVRHMYNSLWGPVVAAMFGFPTSIPTDQGGNFTLVMPYPAHLQLGLKARPIDRVQF
                     NVDAGWTDWSKWNQMTFQFDRSVNLLEMARLFGQADSTKLVIPRGYRSVWSYGYGMQL
                     DVWDGLKLRVGYEPRKSSVPANKIDLIAPMPNLPVKSVGFSYETKDGMKLETGFSYAR
                     GRYNVPANTDCNLNCTDFFNVIYNPYAGLDVSGTLVLRYFGMKITQPF"
     gene            complement(2380..3492)
                     /locus_tag="VIN75_15580"
     CDS             complement(2380..3492)
                     /locus_tag="VIN75_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8975639.1"
                     /translation="MTTANWNGAALALACGLLTSVAAQAQPDNAPATTSPAAPDAPAA
                     APGAPASAPTGSAADALKKQAGDVDQTTLLKDTLSKTDRQYSLLRAGRIAANYDLTYS
                     YVGSQSIDVGFAASTGDIDLLRIETTRSHTITNTLSVDYGLLDNLTVNGTFPFVSKFT
                     QGTEFSGTAYGIGDISLGARYQPFVQNPSGPTFTVTGTLSLPTGRSPFDQRIGQNTST
                     GNGYASGNVGVNASQVFDPVVLFGSLSFGASKSVKNLHQYNPADGSILTGVRPGKTIG
                     FGAGFTYALSYNVSTSVSFQENVTTASRITIVDANNNPVAPRSTSMQTGAVMNFGLGL
                     RINPKTTINLTAGMGLTVDSPDFTLGLNVPLTFAMF"
     gene            complement(3495..4262)
                     /locus_tag="VIN75_15585"
     CDS             complement(3495..4262)
                     /locus_tag="VIN75_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY8975640.1"
                     /translation="MLLILLGVVAAAIIGAGVGSRNEPLNRPPLDYEINVSKMNGGIS
                     PESVQVYPNEYYKYKHIVHQAYDYSCGSAALTTVLKFHLGLNISEQDAMKGMLEHGEQ
                     DKIIARRGFSLLDMKRYVATLGSDSGGFRADMNDLAKLGEPAIVPIDYAGFKHFVVVK
                     GIRDGKVFIADPSAGNIAFSTHEFAGLWDKGTLFVIYPSKTHPIVQSSLSLTDQELGV
                     ISPDLVREDVALRPVDNRDALEHAVQSGLGTLNFKHQ"
     gene            complement(4289..4774)
                     /locus_tag="VIN75_15590"
     CDS             complement(4289..4774)
                     /locus_tag="VIN75_15590"
                     /inference="COORDINATES: protein motif:HMM:NF039674.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HEY8975641.1"
                     /translation="MSARVPAMFRHVALAGMLAAAASGAFAEKKALDDDELRGVSGKG
                     IAIAVNLQLNTRQIAGTAPPSNISAGFNVNGVTTYAIVQNFGGGLQLFSITIDPSVKP
                     DGTGYLAIGMPSYVNAQDFGIHGIGVQTDPNAPIASSLGSLTLNGTASMTGTLNLWPK
                     N"
     gene            complement(4771..5691)
                     /locus_tag="VIN75_15595"
     CDS             complement(4771..5691)
                     /locus_tag="VIN75_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975642.1"
                     /translation="MAPAAALLAPALPVAAATPPEPASHAMQALQDDELSAVRGADGI
                     SFNLNNFSLTSSLTNPLTLTYVSPNGSALTLSGLDLSRTDDADQFADPYQLSLRPTAG
                     GQQMIAIDFPLNAAGNQRWSLTADFANCGSFAAGACAAGASFFGGTLQVLDLTMKGGG
                     LYVAPSQVPNTEGIAFGLGTQLDIGSLSVYSRGRAADGTIDRGDALALTGIHLVDAAT
                     GGVYMLADLDKHPGLINAATDATGSYLHLQIGWPTTGDPVPAATLRIDNITFTTAGIN
                     GGAATATNLGSASIASMQINYLDIKFRTGQ"
     gene            complement(5760..6536)
                     /locus_tag="VIN75_15600"
     CDS             complement(5760..6536)
                     /locus_tag="VIN75_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975643.1"
                     /translation="MTFRPVPAAHATMLACALLVGSAHAQDGGKPRLKALDDAELSGV
                     YGQALLDLTNTSQGGYDFSRLTLNADITMSSTLSGLVLGRHADGTTDIDISTLSFGRS
                     DLDDAHRTVAIANPYFEWVYSGSAATGDRQVVGMRVGFGGIAGDVGLLMNTVSGSLTI
                     NTPNGQMSSLGSQSTTLTGASCGCSLALNQVGGVTAGTTDGASRDFFLSILKSALTYP
                     TTNGLTPAASQAGFWLNWTDRLNALNTTGTVPPNVPKIGP"
     gene            complement(6693..7196)
                     /locus_tag="VIN75_15605"
     CDS             complement(6693..7196)
                     /locus_tag="VIN75_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HEY8975644.1"
                     /translation="MKFFKLATAAALVAASLSASAMTTIADDELSTVAGQDGVSIGAN
                     LNINIGSFQYTDTDANGGTVSFNNISIKGLMHMDVDIINAATFNGILTSVGATLADYD
                     GTSDVVQFAFPNDTLASTAVLPSIKVGSITLGHAGNNAAGTTTSFGSIAINQLDLRGT
                     TVWMWAH"
     gene            complement(7687..8715)
                     /locus_tag="VIN75_15610"
     CDS             complement(7687..8715)
                     /locus_tag="VIN75_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8975645.1"
                     /translation="MVVVPLSQLSAFKAMKDGFAQRGLDLPGILAEAGLNPDPNESVD
                     AEHLADIFSVAWRVAVEQTGDPAVGLSTCPRQPLIGLGSLSSLILSAPDLRSGLQRMA
                     RYTSLISPTTSVGVDMPSSSVGMHVDEDACRITLRISAGKMAVPQQRYDFMAATFLKG
                     MFWLVGEKVNPLRVHHPYAAPADATPWEEAFGCPVHFGAAAFVIELPLSALDIPFPTS
                     DPTVGEMCERMVAQLAAEQGGCITARVRQALMQLISKGDPRRELVAATLCMSERTLQR
                     RLTEEGTSFIELVDDTRRELAQRYFVNGAYSPTEMSFALGFSDPSNFYRACKRWFGRT
                     PNTLRCAA"
BASE COUNT         1352 a         2736 c         3336 g         1561 t
ORIGIN      
        1 gtgccggtgc ccccgccgcc gccgcacgcg acgagcgcga acagggcgcc gagcgcggca
       61 agcgagcggg gcgatgccat ggtcactgcg ccgtggcggc gatctgcagc ttggtgctga
      121 cggcctcggt catggtcttg acgttggcat tgacctgcgg cgagatgtcg gcctgcacct
      181 tgtggccgac ggcgtcttcc atctgcggca ccagcttctc gaacctggcc ttcagcggcg
      241 attccagcag cgccgcgatc tggcgcagct cgtcggcggt gaagttctgc gcgaggatgg
      301 ggccgaccgc cgggttggtg ttcttcttgg cgctggcgac ggcgatgggc atggtcgtgt
      361 cgatgtactt ctgcacgtcg acgccgatct ccttgagggt cttgtcggcg cgctccttgg
      421 gcacgtgggc cgtgtggagc gcgatgttgg actgctgcat cgcgtcgacg ccctggcgct
      481 ggacggcttg caggacgccg ttctcgggat ggaacaccgc caggatgcgg tcgatggcct
      541 tctgcttctc ggcgtccggc gcgggcaggt tggccggggc ggcggcgggc gcggcggcct
      601 tggcctgggc gtgggcggcg gccccggcga cggtcagtgc ggcggcgatg gcgatgtgca
      661 ggaaggagcg gaggggagcg gtcatggtcg ggatccgggg ggcgtcagaa aggttgcgtg
      721 atcttcatgc cgaagtagcg cagcacgagc gtgccggaca cgtccagccc cgcgtacggg
      781 ttgtagatca cgttgaagaa atcggtgcag ttgaggttgc agtcggtgtt ggccggcacg
      841 ttgtagcgtc cccgagcgta gctgaaaccg gtctcgagct tcatgccgtc cttggtctca
      901 tagctgaagc ccacgctctt gaccggcagg ttgggcatcg gcgcgatcag gtcgatcttg
      961 ttggcgggca ccgagctctt gcgcggctcg tagcccacgc gcagcttcag gccgtcccac
     1021 acgtccagct gcatgccgta gccgtagctc cagacgctgc ggtagccgcg cgggatcacc
     1081 agcttggtgg aatcggcctg gccgaacagg cgggccatct ccagcaggtt gacgctgcgg
     1141 tcgaactgga acgtcatctg gttccacttc gaccagtcgg tccagcccgc gtcgacgttg
     1201 aactgcacgc ggtcgatcgg cctcgccttg agcccgagct gcaggtgcgc gggatagggc
     1261 atgaccagcg tgaagttgcc gccctggtcg gtcgggatcg acgtcgggaa gccgaacatc
     1321 gccgcgacca cggggcccca cagcgagttg tacatgtgcc gcacgaactc gggcagcatc
     1381 gggttcgcgt ggaagttgta gtcgccggtg atgaccgtct tggagccgga ctggtagacg
     1441 gcgccgaagc cgagcttgtc cgacggctgc caaagcgcgc cgacgttgaa cgtgacgtcg
     1501 gccggcgccg tgcccgagaa gcgcatctcg ccgatgctgt cgaacggccg caggcggccg
     1561 ctctgcttgc cggtggagtc ggtgccgcac aggccgaagc ccagctcgtc gagcgggttg
     1621 ccgctcggcc cgcaccaggc gtcctgcagc ttgccgatga tgcccagcag cttgttgggg
     1681 aagcgcatct cggtgtcgag cgcgaagccc tggtgcgcga tcgggatcgc cagacccacc
     1741 tggaactggt cgttgacctt gtagcccacc gacggcgaca ggtacacgag gcgctggatc
     1801 accacctgct tgccgtcaaa gcgcgcggga tcgtccgggt tcttggtgcg gtcgatgccc
     1861 accgcctgcg gcacgtaggt gcccgtggcg aaggtccagg gcgagccctc gttgtgccac
     1921 gacagcgcca ggcccgcggc cgcggcgaac ggcaggcgcg ggctgggaac gccggcgatc
     1981 gggatgaaga tcgcctgcct gttgtggtcc gagtgcgtgc cgttgagcgg atcctcggtg
     2041 aagccgccga cgtcgaagcc cgcgggctgg tggaactggg cggtggtgcg cagggcggcg
     2101 ccgaagatcg tgtccgagcg cgtgtccgag gtgatcttcg cgagtccggc cgggttgaag
     2161 tggatcgagt ccagccccgg cggatcggcc gtgacggcgt tgcccagcga catcgccacc
     2221 gggctgaccg ccaggttctc cgtgagggcc gcgtgggcct gcggggacag cagggcgccg
     2281 gccgcgatgc aggtcgacag gatcgtgcgg gccggcggcg tctgccgcga aacgggccgc
     2341 aggcgcgccg ggcgcgtcgt cggtggcacg gtcatggggt cagaacatcg caaaggtcag
     2401 cggcacgttc aacccgaggg tgaagtccgg cgaatcgacc gtcaggccca tgcccgcggt
     2461 caggttgatc gtcgtcttcg ggttgatgcg caggcccagg ccgaagttca tcacggcgcc
     2521 ggtctgcatc gacgtcgagc gcggcgcgac cgggttgttg ttggcgtcga cgatcgtgat
     2581 gcgcgaggcc gtggtgacgt tctcctggaa cgacacgctg gtcgacacgt tgtacgacag
     2641 cgcgtacgtg aagccggcgc cgaagccgat ggtcttgccc gggcgcacgc cggtcaggat
     2701 gctgccgtcg gccgggttgt actggtgcag gttcttgacg gacttggaag cgccgaagct
     2761 cagcgagccg aacagcacca cggggtcgaa cacctgcgag gcgttcacgc cgacgttgcc
     2821 gcttgcgtag ccgttgccgg tcgaggtgtt ctggccgatg cgctggtcga acgggctgcg
     2881 gccggtgggc agcgacagcg tgccggtgac cgtgaaggtc gggccggagg gattctggac
     2941 gaagggctgg tagcgcgcgc ccaggctgat gtcgccgatg ccgtaggcgg tgccgctgaa
     3001 ctcggtgccc tgcgtgaact tgctgacgaa cgggaacgtg ccgttgaccg tcaggttgtc
     3061 cagcaggccg tagtccaccg acagcgtgtt ggtgatcgtg tgcgagcgcg tcgtctcgat
     3121 gcgcagcagg tcgatgtcgc cggtggacgc ggcgaagccg acgtcgatcg actggctgcc
     3181 gacgtagctg taggtgaggt cgtagttggc cgcgatgcgc ccggcgcgca gcagcgagta
     3241 ctggcggtcg gtcttggaca gcgtgtcctt gagcagcgtc gtctggtcga cgtcgccggc
     3301 ctgcttcttg agcgcgtcgg ccgcgctgcc cgtgggcgcg gacgcgggcg cgccgggggc
     3361 ggcggcgggc gcgtccgggg cggcggggct ggtggtggcc ggcgcgttgt ccggttgcgc
     3421 ctgtgcggcg acgctcgtga ggagcccgca cgcgagcgcc agggccgcgc cgttccaatt
     3481 tgccgttgtc atgttcattg gtgcttgaaa ttgagggtgc ccaggccgga ctgcacggcg
     3541 tgctccagcg cgtcgcgatt gtccaccggc cgcagggcca catcctcgcg caccaggtcg
     3601 ggcgagatga cgcccagttc ctggtcggtc agcgacaggc tcgactgcac gatcgggtgc
     3661 gtcttggacg ggtagatcac gaacagcgtc cccttgtccc agagtccggc gaattcatgc
     3721 gtggagaagg cgatgttgcc cgccgacggg tcggcgatga acaccttgcc gtcgcggatg
     3781 cccttgacga cgacgaagtg cttgaagccg gcgtagtcga tcggcacgat ggccggctcg
     3841 cccagcttgg ccaggtcgtt catgtcggcg cgaaagccgc cggagtcgct gcccagcgtc
     3901 gccacgtagc gcttcatgtc cagcagcgag aagccgcggc gcgcgatgat cttgtcctgc
     3961 tcgccgtgct ccagcatgcc cttcatcgcg tcctgctcgc tgatgttcag gcccaggtgg
     4021 aacttcagga ccgtcgtgag cgcggccgag ccgcagctgt agtcgtaggc ctggtggacg
     4081 atgtgcttgt acttgtagta ctcgttcggg tagacctgca ccgactcggg cgagatgccg
     4141 ccgttcatct tcgagacgtt gatctcgtag tccagcggcg ggcggttcag cggctcgttg
     4201 cgcgagccga cgcccgcacc gatgatggcg gcggcgacga cgcccaggag gatcagcagc
     4261 atggtggtct tgccttgcgg tggtcgggct agttcttcgg ccagagattg agcgtgcccg
     4321 tcatcgaggc ggtgccgttg agcgtgagcg agcccaggct gctcgcgatg ggggcgttcg
     4381 ggtcggtctg cacgccgatg ccatggatgc cgaagtcctg cgcgttgacg taggacggca
     4441 tgccgatcgc gaggtagccg gtgccgtcgg gcttgaccga cgggtcgatc gtgatcgaga
     4501 acagctgcag cccgccgccg aagttctgca cgatggcgta ggtcgtcacg ccgttgacgt
     4561 tgaagccggc cgagatgttg ctgggcggcg cggtgccggc gatctggcgc gtgttcagct
     4621 gcaggttcac cgcgatggcg atgcccttgc cggagacgcc gcgcagctcg tcgtcgtcga
     4681 gcgccttctt ctcggcgaac gcgcccgacg ccgcggcggc gagcatgccg gcgagcgcga
     4741 cgtggcggaa catggctggc acgcgtgcgc tcattgaccc gtcctgaact tgatgtcgag
     4801 atagttgatc tgcatggacg cgatgctggc cgacccgagg ttggtcgcgg tcgccgcgcc
     4861 gccgttgatg ccggccgtcg tgaacgtgat gttgtcgatg cgcagcgtgg ccgcgggcac
     4921 cgggtcgccg gtggtcggcc agcctatctg gaggtgcagg tacgagcccg tcgcgtcggt
     4981 ggccgcgttg atcaggccgg ggtgcttgtc gaggtcggcc agcatgtaga cgcccccggt
     5041 ggcggcgtcc accaggtgga tgccggtcag cgcgagcgcg tcgccgcggt cgatcgtgcc
     5101 gtccgcggcg cggccgcgcg agtagaccga caggctgccg atgtccagct gcgtgcccag
     5161 gccgaacgcg atgccttcgg tgttggggac ctgggacggc gcgacgtaca gtccgccgcc
     5221 cttcatcgtg agatcgagca cctgcagcgt gccgccgaag aagctcgcgc ccgcggcgca
     5281 ggcgccggcc gcgaacgaac cgcagttggc gaagtccgcg gtcagcgacc agcgctggtt
     5341 gcccgccgcg ttgagcggga agtcgatcgc gatcatctgc tggccgccgg cggtcgggcg
     5401 cagcgagagc tggtacgggt cggcgaactg gtcggcgtcg tcggtgcgcg acaggtcgag
     5461 gccggacaac gtcagcgcgc tgccgttggg cgacacgtag gtcagcgtca gcgggttggt
     5521 gaggctgctc gtgagcgaga agttgttcag gttgaacgag atgccgtcgg cgccgcgcac
     5581 ggccgacagc tcgtcgtcct gcagcgcctg catcgcgtgg gacgcaggct cgggcggggt
     5641 ggccgcggcg acgggcaggg ccggggcgag cagcgcggcg gccggggcca tggcgcgcag
     5701 caacgcacgg catcgcatcg cgatggcccg tccggtcgcg ctcagtgccc cgctgcgcat
     5761 catggcccga tcttgggcac gttgggcggc acggtgccgg tggtgttgag cgcgttcagg
     5821 cgatcggtcc agttcagcca gaagccggcc tgcgacgcgg cgggcgtgag gccgttggtg
     5881 gtgggatagg tgagggcgga cttcaggatc gacaggaaga agtcgcggct cgcgccgtcg
     5941 gtcgtgcccg ccgtcacgcc gccgacctgg ttcagcgcga ggctgcagcc gcaggaggcg
     6001 ccggtgagcg tcgtcgattg cgatcccagc gagctcatct ggccgttggg cgtgttgatc
     6061 gtgagcgagc cgctgacggt gttcatcagc aggccgacgt cgccggcgat gccgccgaag
     6121 cccacgcgca tgcccaccac ctggcggtcg ccggtcgcgg cgctgcccga gtagacccat
     6181 tcgaagtacg ggttggcgat ggcgaccgtg cggtgcgcgt cgtcgaggtc gctgcggccg
     6241 aagctcagcg tggagatgtc gatgtcggtc gtgccgtcgg cgtggcgtcc cagcaccagt
     6301 cccgagagcg tcgacgacat cgtgatgtcg gcgttcagcg tgagtcgcga gaagtcgtag
     6361 ccgccctggc tcgtgttggt caggtccagc agcgcctggc cgtagacgcc ggacagctcc
     6421 gcgtcgtcga gggccttcag cctgggcttg ccgccgtcct gcgcatgcgc cgatccgacg
     6481 aggagcgcgc aggcgagcat cgtggcgtgg gcggcgggga cagggcggaa ggtcatggcg
     6541 ggattgtcct tggaagcgct ggagagcaaa cccctggacg tgggacgaag ttcgcgcttg
     6601 ccggagaaga aaggccccgt gacggtgatc acggggcctt tcgaggaatg gtccggcccc
     6661 tgatcaagcg ggccggatgt cattcaggca gatcagtgcg cccacatcca cacggtcgtg
     6721 ccgcgcaggt cgagctggtt gatcgcaatc gagccgaacg acgtcgtcgt gcccgcggcg
     6781 ttgttgccgg cgtggcccag ggtgatcgag ccgaccttga tgctcggcag gacggccgtc
     6841 gaggccagcg tgtcgttcgg gaaggcgaac tggacgacgt cggacgtgcc gtcgtagtca
     6901 gccagcgtgg cgccgaccga ggtcaggatg ccgttgaacg tggcggcatt gatgatgtcc
     6961 acgtccatgt gcatcaggcc cttgatgctg atgttgttga agctgacggt gccgccgttg
     7021 gcatcggtgt ccgtgtactg gaacgaaccg atgttgatgt tcaggttggc gccgatcgac
     7081 acgccgtcct ggccggcgac ggtgctcagt tcgtcgtcgg cgatggtggt catggcggaa
     7141 gccgacaggg aagcggcgac cagggcggca gcggtggcaa gcttgaagaa cttcatggtg
     7201 tttgtctctc cagaatagat tgaaatatgg aacctttaaa acccctcgcg agcttgtcag
     7261 gtctgcagcg cccggggtta tgccccgagg tgccgtcgac agtcctgatg aaccgtctgc
     7321 cgccgctgaa accattcctt tgatcgcgtg ccgcaactat gttcgggttc cgtgaactac
     7381 gcactgactt gcggagccaa gcccatggct tgcgaaacca gggatggagg gtaacttccc
     7441 tagtttcggt gagtgactca gggaggcttc ccacgatccg aaacagcggt cgcaccagtg
     7501 aaaagccggg ccctggaatg gctaagtgat aacccgttgg ctcctcgcgc cagcgctggc
     7561 tcgcggggcc agtcggaaaa tcctcggaaa atatcgccgg aaataaaaat ggagcctggc
     7621 ggctccatct tgaaaattgc tttgaatatt ttcccgcaga tccttcaggg aaatgctgga
     7681 taaatatcag gcggcgcagc gcagggtatt cggggtgcgt ccgaaccagc gtttgcaggc
     7741 ccggtagaaa ttgctcggat cagagaatcc gagagcgaag ctcatctcgg tcgggctgta
     7801 ggcgccgttg acgaagtagc gttgcgcgag ctcgcggcgg gtgtcgtcga cgagctcgat
     7861 gaagctggtg ccttcctcgg tgaggcggcg ctgcagcgtg cgttcgctca tgcacagcgt
     7921 cgcggccacc agctcgcggc gcgggtcgcc cttggagatc agctgcatca gcgcctggcg
     7981 cacgcgcgcc gtgatgcagc caccctgttc ggcggccagc tgtgcgacca tgcgctcgca
     8041 catctcgccg acggtcgggt cggaggtggg gaaggggatg tccagcgccg acagcggcag
     8101 ctcgatgacg aaggcggcgg cgccgaagtg caccgggcag ccgaaggctt cctcccacgg
     8161 cgtcgcgtcg gccggggcgg cgtacgggtg gtgcacgcgc agcgggttca ccttttcgcc
     8221 gaccagccag aacatgccct tgaggaaggt ggccgccatg aagtcgtagc gctgctgcgg
     8281 cacggccatc ttgcccgcgg agatgcgcag cgtgatgcga caggcgtcct cgtcgacgtg
     8341 catgccgacg ctggacgacg gcatgtccac gcccacggaa gtcgtcggcg agatcaggct
     8401 cgtgtagcgc gccatgcgct gcaggccgct gcgcaggtcg ggcgcggaca ggatcaggct
     8461 ggacagcgag cccaggccga tcagcggctg gcgcgggcag gtgctcaggc cgacggccgg
     8521 gtcgcccgtc tgctcgacgg ccacgcgcca ggcgacgctg aagatgtcgg ccaggtgctc
     8581 ggcatcgacg gactcgttcg ggtcgggatt gagcccggct tcggccagga tgccgggcag
     8641 gtcgagaccg cgctgcgcaa agccgtcctt catcgccttg aacgcgctga gctgggacag
     8701 gggaacgacg accacggggt actccgattc gctgcgagaa ccggcggccg gatgcaccgt
     8761 cgaatctctt tgccaaaata gaacgaccgt tctatttatt gaatgaaatg ttagacagcg
     8821 ggtccctgca ccgacatggg atgaacccgc agtccgcccc gtcccggggc ggtcccgacc
     8881 cgttccgggg tgctttcccc ccaactttgg tgaagggtaa gtaccttgtg tcgcgccgag
     8941 gccgaaacag ctcgtttccc gggcgtggga tggttgcagg cgggg
//
LOCUS       DATCRO010000567         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_692879, whole genome shotgun sequence.
ACCESSION   DATCRO010000567 DATCRO010000000
VERSION     DATCRO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692879"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1204
                     /locus_tag="VIN75_15615"
     CDS             209..1204
                     /locus_tag="VIN75_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEY8975646.1"
                     /translation="MFSHNKRLQYTVRVDETNPGLANLMLEQFGGPQGELAAACRYFT
                     QYLAEEDPGRRDMLIDIATEELSHLEVIGTIVAMLNRGAKGQLSEAMQGEAELYRSIS
                     GAGNDSHVTQVLYGGGPALVNSGGVPWTAAYIDTIGDPTCDLRSNIAAEARAKIIYER
                     LINCTDDPGVKDALTFLMTREISHQLSFEKALYSIEPNFPPGKLPGKQEFANVYFNMS
                     RRTSDGLSAGDAGSSATGGAATSGTDGNAELATDRRGPWNASPVFNYQEGRPAVDGGS
                     GGFEVQCDPEEGQALQAMKQRLASVVDSDPITGAELGSAKPAAGDGAGAGASAKQ"
     gene            1269..2606
                     /locus_tag="VIN75_15620"
     CDS             1269..2606
                     /locus_tag="VIN75_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY8975647.1"
                     /translation="MKDIAMTRALDTAPGAAHPWPGGPASGRPGLDVRAFARRCRAFA
                     GPGCMVAVGYMDPGNWATSIAGGSRYGETLLAVVIASSLMAMLFQAAAVRLGLAGGED
                     LAQACRRHFGRRTSLALWLFSEVAIVACNLAEVLGMAIGLNLLLGLPMFAGVCITVVD
                     VLLVLALRARGFRLVEAVVGLLVAAITACFIAQLAWARPSAQAILQGLALAPRIVADP
                     QMLYLAVGIVGATVMPHNLYLHSAIVRTRAGGRDAASVRSAIRWSTVDSTLALGLALF
                     VNAAILVVAAAAFHRPGQAVVTELSDAYRLISPTLGVALASTVFGIGLLASGLSASIT
                     GTLAGQVVMEGFMDIRLSPARRALLTRALAIGPAAAATAWFGARGASGLLVFSQVVLS
                     LQLPFALVPLLMFTTRRRHLGMHAFGRAASLALWAAAVLVICLNAWLLARLFS"
     gene            2718..3275
                     /locus_tag="VIN75_15625"
     CDS             2718..3275
                     /locus_tag="VIN75_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8975648.1"
                     /translation="MATAPRKTSTSARKPAAKTARGASRTDAIALLRADHKRVSELFE
                     QYESTRSAARKKTLVDTICQELTVHAQIEEEIFYPAVKAALKDKELVPEAQVEHQSLK
                     DLIAQVKGKEPDGEMFDAKIKVMSEYVKHHVKEEQNEMFVRAKKTRLDMQQLGEQLAA
                     RKQALMDDPAQLEASPTEQPAENFA"
BASE COUNT          528 a         1295 c         1246 g          505 t
ORIGIN      
        1 ccgttgcgtg cgtcgccaaa aacgggccgg cgctgcccgg atgctcgctc agccacgcgg
       61 gccctcctcg actccgcccg tcgcgagcga accgccgtca gcgcgcgttc ggccctggaa
      121 cccgcattgc cacgtgtcgc cgtcgcccgc ttcgcgacgt ttcagtcatc ccctcatcgc
      181 cgatcgaatc ggaacaggaa ggccgctcat gttctcgcac aacaaacgtc tccagtacac
      241 cgtccgcgtc gacgagacca accccggtct cgccaacctg atgctcgagc agttcggtgg
      301 cccgcagggc gagctcgccg ccgcatgccg ctacttcacg caatacctcg ccgaggagga
      361 cccgggccgg cgcgacatgc tgatcgacat cgccaccgag gagctgagcc acctcgaggt
      421 gatcggcacc atcgtcgcga tgctcaatcg cggcgcgaag gggcagctgt ccgaggcgat
      481 gcagggcgag gccgagctgt accgctccat cagcggcgcg ggcaacgact cgcacgtgac
      541 gcaggtgctg tacggcggcg gcccggcgct cgtcaactcg ggcggggtgc cgtggacggc
      601 cgcctacatc gacaccatcg gtgacccgac ctgcgacctg cgctcgaaca tcgccgccga
      661 ggcgcgcgcc aagatcatct acgagcggct catcaactgc accgacgatc ccggcgtcaa
      721 ggacgcgctc accttcctga tgacgcgcga gatctcgcac cagctgtcgt tcgagaaggc
      781 gctgtactcg atcgagccca acttcccgcc gggcaagctg cccggcaagc aggagttcgc
      841 caacgtctac ttcaacatgt cgcgccgcac gagcgacggc ctgtcggccg gcgacgccgg
      901 cagcagcgcg acgggcggcg cggcgacgag cggcaccgac ggcaacgcgg agctcgcgac
      961 ggaccgccgc ggcccgtgga acgcgtcgcc ggtcttcaac taccaggagg gccgcccggc
     1021 cgtggacggc ggcagcggcg gcttcgaggt gcagtgcgat cccgaggaag gacaggcgct
     1081 gcaggcgatg aagcagcggc tcgcctcggt ggtcgacagc gatccgatca ccggcgcgga
     1141 gctcggcagc gccaagccgg ccgcgggcga cggcgccggc gccggcgcct cggccaagca
     1201 gtgaccgtgc gccggacgcg accggcgcga cgagcccgcg acgcgcgcgg gcccgccggc
     1261 ggatatccat gaaggacatc gcgatgacac gggcgctgga cacggcgccc ggtgcggcac
     1321 atccctggcc gggcggcccc gcctccggtc gcccgggcct ggacgtgcgc gcgttcgcgc
     1381 gccgctgccg cgccttcgcg ggccccggct gcatggtggc ggtcggctac atggatccgg
     1441 gcaactgggc gacgtcgatc gcgggcggct cgcgctatgg cgagacgctg ctggccgtcg
     1501 tcatcgcctc cagcctgatg gcgatgctgt tccaggccgc cgccgtccgc ctgggcctgg
     1561 caggcggcga ggacctggcg caggcctgcc gccgccattt cggacgccgc accagcctcg
     1621 ccctgtggct gttcagcgag gtcgcgatcg tcgcctgcaa cctggccgag gtgctgggca
     1681 tggcgatcgg gctgaacctc ctgttgggcc tgccgatgtt cgcgggcgtg tgcatcaccg
     1741 tcgtcgacgt gctgctcgtg ctcgcactgc gggcacgcgg gtttcggctg gtcgaggcgg
     1801 tcgtgggcct gctggtggcc gcgatcacgg cctgcttcat cgcccagctc gcctgggcca
     1861 ggccctcggc ccaggccatc ctgcagggcc tggcgctcgc gccacgcatc gtggccgacc
     1921 cgcagatgct ctacctggcg gtgggcatcg tgggagcgac ggtgatgccg cacaacctgt
     1981 acctgcattc ggcgatcgtg cgcacgcgcg caggcgggcg cgacgcggcc agcgtgcgat
     2041 cggcgatccg ctggtcgacc gtcgattcca cgctcgcgct ggggctggcc ctgttcgtca
     2101 acgcagccat cctggtcgtc gccgcggccg ccttccaccg gccggggcag gctgtcgtga
     2161 ccgaactgtc cgatgcctac cggctgatct cgccgacgct gggcgtggcg ctggcgagca
     2221 ccgtgttcgg catcggcctg ctcgcctccg ggctgagcgc gtccatcacc ggcacgctgg
     2281 ccggacaggt cgtgatggag ggcttcatgg acatccgcct gtcgcccgcg cggcgagcgc
     2341 tgctcacgcg cgcgctcgcg atcgggcccg cggcggcggc cacggcctgg ttcggcgccc
     2401 gcggggcgag cggcctgctc gtcttcagcc aggtcgtgct gagcctgcaa ctgccgttcg
     2461 cgctggtgcc gctgctcatg ttcaccacgc gccggcgcca tctgggcatg catgcgttcg
     2521 gacgtgcggc aagcttggca ctgtgggccg ccgcggtgct cgtgatctgc ctgaacgcct
     2581 ggttgctggc gcgcctgttc tcctgaggaa cctggcttca cgggctcgcg cgcacttcgt
     2641 cggcgcgcgg cttgccaccg tcgagccgcg cgctcggccg cgcatccaac ccatccatcg
     2701 acaggagctt caagatcatg gcaactgcac cccgcaagac atccacgtcc gcccgcaagc
     2761 ccgccgccaa gaccgcgcgc ggcgcatcgc gcacggacgc catcgcgctg ctgcgcgccg
     2821 accacaagcg cgtcagcgag ctgttcgagc agtacgagtc gacccgatca gcggccagga
     2881 agaagacgct ggtggacacc atctgccagg aactgaccgt ccacgcgcag atcgaggagg
     2941 agatcttcta tccggccgtc aaggccgcgc tcaaggacaa ggagctcgtc cccgaggcgc
     3001 aggtcgagca ccagagcctc aaggacctga tcgcgcaggt caagggcaag gagccggacg
     3061 gcgagatgtt cgacgccaag atcaaggtga tgtcggagta cgtcaagcac cacgtcaagg
     3121 aagagcagaa cgagatgttc gtccgcgcga agaagacccg cctggacatg cagcagctcg
     3181 gcgagcagct ggccgcccgc aagcaggcgc tgatggacga cccggcgcag ctcgaggcat
     3241 cgcccaccga gcagccggcc gagaacttcg cctgagacca cggcgctgga tggaagacca
     3301 gcgccggtgg caaggacttc ggccgcgcct tgccacggcg ttcacttgtt gccggacggt
     3361 gggcacgtcg tgaggcgcgt ggcgatggcg gtcgatcgac ctggcgtcag cgcccgccgt
     3421 ccttctcgac gtgcggatcg tgcgccgcat ggtccgcggt gggcgccggc tcgccttcga
     3481 cgacgggccc ggcgacgggc ccggtgcccg cggcgccgga gcccagcacg ccgcccgcga
     3541 tcgcgccgcc gacggcaccg gcgaggctgc tcac
//
LOCUS       DATCRO010000568         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_197409, whole genome shotgun sequence.
ACCESSION   DATCRO010000568 DATCRO010000000
VERSION     DATCRO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197409"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VIN75_15630"
     CDS             complement(<1..279)
                     /locus_tag="VIN75_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975649.1"
                     /translation="MIIDAHLHCTGDETTADVLDALDEADIDIGVLIAPFLSNGYSMA
                     DAESIRRANQHLAHLVRGHGDRLVGLAIVNPSLPGAADELRRSVETLGL"
     gene            complement(293..1582)
                     /locus_tag="VIN75_15635"
     CDS             complement(293..1582)
                     /locus_tag="VIN75_15635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8975650.1"
                     /translation="MQTSPPTWTIATPTGAPDRAARATLIVLSHLRWGFVFQRPQHLM
                     TRLAKHFDVIFVEEPVLADGAPVLTSAVHEPGVEVVTPRTSVVAPGFHDDQMGVLKPL
                     LADFVASRGIVDPLVWLYTPMALPLVADLEPAAVVYDCMDDLASFKFAPPQLVEREKA
                     LMELADVVLTGGPSLYEARKDRHPNIHCLPSAVDAAHYTPGSLDRGSVEALRAAELHR
                     GLPRPRLGFFGVIDERLDLELVAGIADRRPDWQIVMVGPVVKIDPAMLPQRPNIRWLG
                     MQRYEALPHLMSRWDVCLLPFALNEATRFISPTKTLEYLAGGKPVVSTRIRDVESLYA
                     AAVRFGSTADEFVAAIEAALAQSDDENRDWRARAQALVDGCTWDGTAAIIVNLLMDYV
                     PERAPAPALRAAPEDGAPLAVPLPVEIDAQSVRPLVA"
     gene            1817..>3707
                     /locus_tag="VIN75_15640"
     CDS             1817..>3707
                     /locus_tag="VIN75_15640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8975651.1"
                     /translation="METQDKPTPSPTRRLQASEERFRLLVESVRDYAIFMLDPEGRVI
                     TWNQGAERFMGYQPEEVIGSHFCRFYPPDALAKGVPGEELRRASEDGSFEDEGWRLRK
                     DGSMFWANVVITAMRDESGKLVGYAKVTRDLTQRRNQEEALRISEERFRLLVEGVSDY
                     AIFMLDVNGHVASWNVGAQRIKGYTAEEIVGRHFSVFYPREVVDSGWPEHELREAAAT
                     GRFIDTGWRVRKDGSMFWANVTITAMRDDTGKLLGYAKLTRDLTERKRAEAMEMASQE
                     REEILDAERNARISAQRATRLKDEFLATLSHELRTPLSAILGWTQVLLKGHAVSPVDQ
                     RRAIEVIDRNARAQVQLIGDLLDLSRIMSGKLRLDLQQVSIHDVVQAALDSAAPAADA
                     KEIRLVSLLDPARAIVNGDAGRLQQVVWNLLTNAIKFTPRGGRVQVLLQRVNSHVELS
                     VADTGIGIPANFLPQVFDRFSQRDGSSTRAHGGLGLGLAICKQLVELHGGSIRVASPG
                     EGQGATFSVDLPVSIMQVESERERTHPTAEGGESDAVAMPRLDGIQVFVIDDEPDARE
                     LLQRVLTEQGANVTLFDSADSALAAMATSRPTIIVSDVGMPGADGYQMMRTLRASESR
                     DSRIPA"
BASE COUNT          586 a         1289 c         1327 g          505 t
ORIGIN      
        1 caggcccagc gtctcgacgg agcggcgcag ctcgtcggcc gcgcccggca gcgacgggtt
       61 gacgatcgcc aggccgacga gccggtcccc atggccgcgc acgaggtggg ccaggtgctg
      121 gttggcgcgc cggatcgact cggcgtcggc catcgagtag ccgttgctca ggaacggggc
      181 gatgagcacg ccgatgtcga tgtccgcctc gtcgagcgcg tcgaggacgt cggccgttgt
      241 ttcgtcgccg gtgcagtgca ggtgagcgtc gatgatcacg gccggcgccc gttcacgcga
      301 ccagcggtcg aacggactgc gcgtcgatct cgacgggcag cggcaccgcc agcggcgcgc
      361 cgtcctccgg cgcggcccgc agcgccggcg cgggcgcccg ctccgggacg tagtccatca
      421 gcaggttcac gatgatcgcc gcggtgccgt cccaggtgca accgtcgacc agcgcctgcg
      481 cccgtgcacg ccagtcgcgg ttctcgtcgt cggactgcgc cagcgcggcc tcgatcgcgg
      541 ccacgaattc gtcggccgtc gagccgaagc gcaccgcggc ggcgtacagc gactcgacat
      601 cccggatccg cgtgctgacg accggcttgc cgccggccag gtattccagg gtcttggtcg
      661 ggctgatgaa gcgcgtggcc tcgttgaggg cgaagggaag caggcacacg tcccagcgcg
      721 acatcaggtg cggcagtgcc tcgtagcgct gcatgcccag ccaacggatg ttggggcgtt
      781 gcggcagcat ggccggatcg atcttgacga cggggcccac catcacgatc tgccagtcgg
      841 gccggcgatc ggcgatgccg gccaccagct cgaggtcgag ccgctcgtcg atcacaccga
      901 agaagcccag gcgcggacgc ggcagtccgc gatggagctc ggccgcccgc agggcttcga
      961 cggagccgcg atcgaggctc cccggtgtgt agtgggcggc gtcgacggcg ctgggcaggc
     1021 aatggatgtt ggggtgccga tccttgcgcg cctcgtacag cgacgggccg ccggtcagaa
     1081 cgacgtcggc cagttccatc aacgccttct cgcgctcgac cagctgcggc ggcgcgaact
     1141 tgaacgaggc caggtcgtcc atgcagtcgt agacgacggc agccggctcg aggtcggcca
     1201 cgagcggcag ggccatcggc gtgtagagcc agaccagcgg atcgacgatg ccgcgcgagg
     1261 ccacgaagtc cgccagcagc ggcttgagca cgcccatctg gtcgtcatgg aagccgggcg
     1321 ccacgacgct ggttcgcggc gtgaccacct cgacgccggg ctcgtgcacg gcgctcgtca
     1381 gcacgggcgc gccgtcggcc aggaccggct cctcgacgaa gatcacgtcg aagtgcttgg
     1441 cgaggcgggt catcaggtgc tggggccgct ggaacacgaa tccccagcgc aggtgcgaca
     1501 ggacgatcag ggtcgcgcgc gctgcgcgat cgggcgctcc ggtgggcgtg gctatcgtcc
     1561 aggtcggcgg agacgtctgc atggctgaaa ctcccgatat tgttgaaaag ttggaatgac
     1621 cccgaggcaa gcgtcatgcc tcggacgaac gatcagggcc ccttggccgc aggctggcgc
     1681 ggatggggaa tgccccgctg cgcagggcgc gcgcatgggc ctaacatgcc gctgcgtgcg
     1741 tcccgcccga ccctggcacg ccgcctgccg cacgcgccgc gcacggtcga cttcgtcgtc
     1801 catcggcgcc aggaccatgg aaacccaaga caagccgacc cccagcccca cccgccgcct
     1861 gcaggccagc gaggaacgct ttcgcctgct ggtcgagagc gtccgcgact acgcgatctt
     1921 catgctcgac cccgagggtc gcgtgatcac ctggaaccag ggcgccgagc gcttcatggg
     1981 ctaccagccc gaggaggtca tcggcagcca cttctgccgc ttctacccgc ccgacgcgct
     2041 ggccaagggc gtgccgggcg aggaattgcg gcgcgccagc gaggacggct ccttcgagga
     2101 cgaaggctgg cgcctgcgca aggacggctc gatgttctgg gccaacgtcg tgatcacggc
     2161 gatgcgcgac gagtcgggca agctcgtcgg ctacgcgaag gtgacgcgcg acctgacgca
     2221 gcggcgcaac caggaagagg cgctgcgcat cagcgaggag cgcttccgcc tgctggtcga
     2281 gggggtgtcg gactatgcga tcttcatgct cgacgtcaac ggccatgtcg ccagctggaa
     2341 cgtcggcgcg cagcgcatca agggctacac ggccgaggag atcgtcggcc ggcacttctc
     2401 ggtcttctac ccgcgcgagg tggtcgacag cggctggccg gagcacgagc tgcgcgaggc
     2461 cgcggccacc ggacgcttca tcgacacggg ctggcgcgtg cgcaaggacg gctcgatgtt
     2521 ctgggccaac gtcaccatca ccgcgatgcg cgacgacacg ggcaagctgc tgggctacgc
     2581 caagctcacg cgcgacctga ccgaacgcaa gcgcgccgag gccatggaga tggccagcca
     2641 ggagcgcgag gagatcctgg acgccgagcg caacgcccgc atctcggcgc agcgcgccac
     2701 gcgcctgaag gacgagttcc tcgcgacgct gtcgcacgag ctgcgcacgc cgctgagcgc
     2761 gatcctgggc tggacccagg tgctgctcaa gggacatgcc gtcagcccgg tcgaccagcg
     2821 gcgcgccatc gaggtcatcg accgcaacgc gcgcgcccag gtgcagctga tcggcgacct
     2881 gctggatctc agccgcatca tgtcgggcaa gctgcggctc gacctgcagc aggtctcgat
     2941 ccacgacgtc gtgcaggccg cgctcgattc ggccgcgccg gccgccgacg ccaaggagat
     3001 ccgcctggtg agcctgctcg acccggcgcg cgcgatcgtc aacggggacg ccgggcggct
     3061 gcagcaggtg gtgtggaacc tgctcaccaa cgcgatcaag ttcacgccgc gcggcggccg
     3121 cgtgcaggtg ctgctgcagc gcgtgaactc ccacgtggag ctgagcgtgg cggacaccgg
     3181 catcggcatc cccgccaact tcctgccgca ggtgttcgac cgcttctcgc agcgcgacgg
     3241 ctcgagcacg cgcgcgcacg gcggcctcgg gctgggcctg gcgatctgca agcagctggt
     3301 cgagctgcac ggcggcagca tccgcgtcgc gagccccggc gagggccagg gcgcgacgtt
     3361 ctcggtcgac ctgccggtgt cgatcatgca ggtcgaatcg gaacgcgaac gcacccatcc
     3421 gaccgccgag ggcggcgagt cggacgccgt cgcgatgccg cggctcgacg gcatccaggt
     3481 gttcgtcatc gacgacgagc ccgacgcgcg cgagctgctg cagcgcgtgc tgaccgagca
     3541 gggcgccaac gtgacgctgt tcgactcggc cgacagcgcg ctcgccgcga tggccacgtc
     3601 gcggcccacc atcatcgtca gcgacgtcgg catgccgggc gccgacggct accagatgat
     3661 gcgcacgctg cgcgccagcg agtcgcgcga ctcgcgcatc ccggcgc
//
LOCUS       DATCRO010000569         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_568855, whole genome shotgun sequence.
ACCESSION   DATCRO010000569 DATCRO010000000
VERSION     DATCRO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568855"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2517
                     /locus_tag="VIN75_15645"
     CDS             <1..>2517
                     /locus_tag="VIN75_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY8975652.1"
                     /translation="GDEPIAIIALAGRFPGARDVEQFWANLCEGRDSITHFGDGQLDP
                     SLAASLVSDPDYVKARGIVDDVEMFDPAFFGMSPREAELMDPQQRIFMELCWEALERA
                     GHVPDADEMPIGVFGGMYNATYYRHHLQPRPDLVELLGEFPVMLANEKDYIATRAAYR
                     LNLTGPAVNVFTGCSTSLVAIAQAFWSLRQGDCRMAVAGGASITCPPRSGYLHQAGSM
                     LSPDGRTRTFDARSGGTVFSDGAAVVVLKRLSDAICDGNEVIAVIRGVGLSNDGRNKA
                     SFTAPSVDGQAAAIERAQRVAGVPARSISYVEAHGTATPMGDPVEIEALNQAFRRSTA
                     DQAFCRIGSLKSNVGHLVIAAGAAAVIKTALALQREQLPATIHYTSPNPAIPFEGSPF
                     VVNDRPHSWPRATAPRRAGVSSFGVGGTNAHAILEEAPLREPSSRAQGAQRLQLAARS
                     AAALEAMATRLADHLEANPDANLADVAHTLQHGRSRFVHRTAIVAADVAEAVAALRTR
                     GHASRVSGALGAKTPATVWMFPGQGAQYAGMGLALARADAAFGRAFDEALDAVAPHLS
                     FDLRARLADPSPDALRATEATQPALFCIEVALARAWQARGLQPAALVGHSIGEFPAAV
                     IAGVMSLADAARLVARRGALLQAQPSGAMLSVRLPLADALALAPADVFLAAENGPRAC
                     VFAGSHEAIAAWTTVLEQSGVAARALVTSHAFHSPAMDAAVAPFEMLLRGVPLSAPRI
                     PIVSTATGQRLTDAQATDPEYWATQLRKPVLFSRALLAAQAAHEGAVLLEMGPRETLC
                     TLARQHAAPGRPAAVAIASLGDSPASEAQRPMLADAQLWTC"
BASE COUNT          316 a          948 c          917 g          336 t
ORIGIN      
        1 ggcgacgagc cgatcgccat catcgcgctg gccggccgct tccccggcgc gcgcgacgtc
       61 gagcagttct gggccaacct gtgcgaaggc cgcgacagca tcacccactt cggcgacggc
      121 cagctcgatc cgtcgctggc ggcctcgctg gtttccgatc ccgactacgt caaggcacgc
      181 ggcatcgtcg acgacgtcga gatgttcgac ccggccttct tcggcatgtc gccgcgcgag
      241 gccgagctga tggacccgca gcagcgcatc ttcatggagc tgtgctggga ggcgctggag
      301 cgcgcaggcc acgtgcccga cgccgacgag atgcccatcg gcgtgttcgg cggcatgtac
      361 aacgccacgt actacaggca ccacctgcag ccgcggccgg atctggtcga gctgctgggc
      421 gagttcccgg tgatgctggc caacgagaag gattacatcg cgacgcgcgc ggcctaccgc
      481 ctgaacctga cgggcccggc ggtcaacgtg ttcaccggat gctcgacgtc gctggtcgcc
      541 atcgcccagg ccttctggag cctgcgccag ggcgactgcc gcatggcggt ggccggcggc
      601 gcgtcgatca cctgcccgcc gcgcagcggc tacctgcacc aggcgggctc gatgctgtcg
      661 ccggacggtc gcacgcggac gttcgacgcc cgctccggcg gcaccgtctt cagcgacggc
      721 gccgcggtcg tggtgctcaa gcggctgagc gacgcgatct gcgacggcaa cgaggtgatc
      781 gccgtgatcc gcggggtggg cctcagcaac gacggccgca acaaggcgag cttcacggcg
      841 ccgagcgtcg acggccaggc ggcggcgatc gagcgcgccc agcgcgtcgc gggcgtgccg
      901 gcgcgcagca tctcgtacgt cgaggcgcac ggcaccgcca cgccgatggg cgacccggtc
      961 gagatcgagg cgctgaacca ggccttccgc cgcagcacgg cggaccaggc cttctgccgc
     1021 atcggttcgc tcaagagcaa cgtcggccac ctcgtgatcg cggccggcgc ggcggcggtg
     1081 atcaagacgg cgctcgccct gcagcgcgag cagttgcccg cgacgatcca ctacacgagc
     1141 ccgaacccgg cgatcccgtt cgagggctcg cccttcgtgg tgaacgaccg cccgcactcg
     1201 tggccgcgcg cgaccgctcc gcgccgcgcc ggcgtcagca gttttggcgt gggcggcacc
     1261 aacgcccacg cgatcctgga agaagcaccg ctgcgcgagc cctccagccg cgcgcagggc
     1321 gcgcagcgcc tgcaactggc cgcgcgttcg gcggcggcgc tggaggcgat ggcgacgagg
     1381 ctggccgacc atctcgaggc gaatcccgat gcgaacctcg cggacgtcgc gcacacgctg
     1441 caacacggcc gctcgcgctt cgtgcatcgc acggccatcg tggcggccga cgtggccgag
     1501 gccgtcgccg cgctgcgcac gcgcgggcac gcgtctcgcg tgtcgggcgc gctcggcgcg
     1561 aagacgccgg ccaccgtctg gatgttcccg ggccagggcg cgcagtacgc cggcatgggc
     1621 ctggcgctcg cgcgtgccga cgccgccttc ggccgcgcgt tcgacgaggc gctggacgcc
     1681 gtcgcgccgc acctgagctt cgacctgcgc gcacgcctgg ccgacccatc gcccgacgcg
     1741 ttgcgcgcga ccgaggcgac gcagcccgcg ctgttctgca tcgaggtcgc gctcgcgcgc
     1801 gcctggcagg cgcgcggcct gcagccggca gcgctggtcg gccacagcat cggcgagttc
     1861 ccggcggcgg tgatcgcggg cgtgatgtcg ctcgccgacg ccgcgcgcct cgtcgcgcgg
     1921 cgcggcgcgc tgctgcaggc gcagccgtcc ggcgcgatgc tgtcggtgcg cctgccgctg
     1981 gccgacgcgc tggcgctggc tcccgccgac gtcttcctgg ccgccgagaa cggcccgcgc
     2041 gcctgtgtgt tcgcgggctc gcacgaggcg atcgccgcgt ggacgaccgt gctggagcag
     2101 tccggcgtcg cggcgcgcgc gctggtcact tcgcacgcgt tccactcgcc ggcgatggat
     2161 gccgcggtcg cgccgttcga gatgctcctg cgcggcgtgc cgctgtcggc gccgcgcatc
     2221 cccatcgtct cgaccgccac cggccagcgc ctgaccgacg cgcaggccac cgaccccgag
     2281 tactgggcga cgcagctgcg caagccggtg ctgttctcgc gcgcgctgct ggccgcgcag
     2341 gcggcgcacg agggcgccgt gctgctggag atgggcccgc gcgagacgct gtgcacgctg
     2401 gcacgccagc acgcggcgcc gggccgtccc gcggccgtgg ccatcgcgtc gctgggtgat
     2461 tcgcccgctt ccgaggcgca gcgcccgatg ctggccgacg cgcagctgtg gacgtgc
//
LOCUS       DATCRO010000570         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_186606, whole genome shotgun sequence.
ACCESSION   DATCRO010000570 DATCRO010000000
VERSION     DATCRO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186606"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VIN75_15650"
     CDS             <1..948
                     /locus_tag="VIN75_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8975653.1"
                     /translation="LEEITDGTLAIGDRVVNDVSPSDRGVAMVFQSYALYPHMTVGEN
                     MGFALKMAGRPKAEIAAAVGRAAEILQISALLDRKPKALSGGQRQRVAIGRAIVRQPQ
                     VFLFDEPLSNLDAALRVQMRTELARLHRELGTTMVYVTHDQVEAMTLGQRIAVFNAGR
                     IAQVGEPMALYDRPANLFVAGFLGSPKMNLVPVTVSANDPAGLRVRAHEGGGEFVLGA
                     PAAGAAPGAAATLGFRPDQVELAAAGAGALEGQVDLVERLGDATLVHVRCAGQTAPVI
                     VRLPGHAEPLAAGSRVALRPDLARSRLFDSAGEALQLSA"
     gene            complement(1194..2486)
                     /locus_tag="VIN75_15655"
     CDS             complement(1194..2486)
                     /locus_tag="VIN75_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975654.1"
                     /translation="MRAFHSRAALALTLAAGLAATLPAAAADTAAAAASQPANTVRPA
                     FGSAFNTAQEALKAGNGAVALAKLKEIEAIPDLNPYEKYLILRLRGPAEYSLNDYTAA
                     AADYDAILANPLLPPEDRVAMLKALAGIYYGSENYPKAAVAIQRYLDAGGNDPQLKEL
                     LPQAQYAAKDYANAAKGFRAEVDATYAAGRVPTEKLLRLEYSAYVGLKDDAGVVSAME
                     HLAVNYPKADYWRDLIPRVREVDNFSERLVLDYYRLKAQVFGQVDDRERLNYAAIAAR
                     AGYPGEAKKVLDAAAAGKPFAGNEVADANRLRSEITKAAAADVAQQNANETAARNAKD
                     GNALVSQGLLETVTDNAAKGAQLIEQGIAKGGLKQPEDAKLHLGYAQVRAGRDADALK
                     TFQSVKGPNGLTELAHMWVLYLESKQAPAAAPAAAASH"
     gene            complement(2703..3443)
                     /locus_tag="VIN75_15660"
     CDS             complement(2703..3443)
                     /locus_tag="VIN75_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8975655.1"
                     /translation="MSALAQLLNTLDPDGSQPLYQQLQRALREAIEKRVLGPDDALPS
                     ERQLAADLGVSRITVRKAIQGLADEGFLDQRQGSGNFVSTRIDKNFAKLTSFSEDMRA
                     RGRTPRSEWIKRSSGTVTPEEAMKLALSPGSLVYRFHRLRYADDAPMALEYATLSAAA
                     LPSLESVDNSLYDALEKAGNRPVRALQRLRALLLNEEQARLLDADVGDAGLLVERLGY
                     LRDGRAIELSQSIYRGDTYDFVAELNAS"
BASE COUNT          548 a         1277 c         1426 g          598 t
ORIGIN      
        1 ctcgaggaga tcaccgacgg cacgctggcg atcggcgatc gcgtcgtcaa cgacgtctcg
       61 ccgtccgacc gcggcgtggc gatggtgttc cagagctacg cgctgtaccc gcacatgacg
      121 gtcggcgaga acatgggctt cgcgctgaag atggccgggc gccccaaggc cgagatcgcc
      181 gcggcggtcg gtcgcgccgc ggagatcctg cagatcagcg cgctgctcga tcgcaagccc
      241 aaggcgctgt cgggcggcca gcgccagcgc gtggcgatcg gccgggcgat cgtgcgccag
      301 ccgcaggtgt tcctgttcga cgagccgctg tccaacctcg acgcggcact gcgcgtgcag
      361 atgcgcaccg agctcgcgcg cctgcaccgc gaactgggca cgacgatggt ctacgtgacg
      421 cacgaccagg tcgaggccat gacgctgggc cagcgcatcg ccgtgttcaa tgccgggcgc
      481 atcgcgcagg tgggcgagcc gatggcgctg tacgaccgcc ccgccaacct gttcgtggcc
      541 ggctttctcg gctcgccgaa gatgaacctg gtgcccgtga ccgtgagcgc caacgacccc
      601 gcggggctgc gtgtgcgcgc gcacgagggc ggcggcgagt tcgtgctcgg cgcgccggcc
      661 gcgggcgccg cgcccggcgc tgccgcgacg ctgggcttcc gtcccgacca ggtggaactc
      721 gcggccgccg gcgcgggcgc gctcgagggc caggtcgatc tcgtggagcg cctgggcgac
      781 gccacgctgg tgcacgtgcg ctgcgccggc cagaccgcgc cggtcatcgt gagactgccc
      841 ggccacgccg agccgctggc cgcgggcagc cgggtcgcgc tgcggccgga cctggcgcgc
      901 agccgcttgt tcgacagcgc gggcgaggcg ttgcagctca gcgcctgagg cggcccctgc
      961 ccatgaatgg ggtcttcgcc gatctgcggg gatctggtcg acgcgaggca cggctcctac
     1021 gaagaacaaa cgtgtcatcc tgcgactgcg cgcaggatga catcgcgccg gggcagacgc
     1081 cctttgcgat gcggccttct ccgacccgcc gcggagtcac actccccgcg aatcggcgat
     1141 gaatcgtgaa aaaggccccg cgagcggggc cttgtcgttg ctgcgagccg cgcttagtgc
     1201 gacgcggcag ccgccggcgc ggccgcggga gcctgcttgg actccaggta cagcacccac
     1261 atgtgcgcga gttcggtcaa gccgttgggg cccttgaccg actggaacgt cttgagcgca
     1321 tcggcgtcgc ggcccgcgcg cacctgcgcg tagcccaggt gcagcttggc atcctcgggc
     1381 tgcttgagac cgcccttggc gatgccctgc tcgatcagct gcgcaccctt ggcggcgttg
     1441 tcggtgaccg tctcgagcag gccctgcgac accagcgcgt tgccgtcctt ggcgttgcgc
     1501 gcggccgtct cgttggcgtt ctgctgcgcc acgtcggccg cggcggcctt ggtgatctcg
     1561 gagcgcaggc ggttcgcgtc ggcgacctcg ttgcccgcga acggcttgcc ggcggcggcg
     1621 gcgtccagca ccttcttggc ttcgcccgga tagcccgcgc gcgcggcgat ggcggcgtag
     1681 ttcaggcgct cgcggtcgtc gacctggccg aacacctgcg ccttcaggcg gtagtagtcg
     1741 aggacgaggc gctcggagaa gttgtcgacc tcgcgaacgc gcgggatcag gtcgcgccag
     1801 tagtcggcct tcgggtagtt gacggccagg tgttccatcg cggagacgac gccggcgtcg
     1861 tccttcaggc cgacgtacgc gctgtactcg agccgcagca gcttctcggt gggcacgcgg
     1921 cccgcggcgt aggtggcgtc cacctcggcg cggaagccct tggcggcgtt cgcgtagtcc
     1981 ttggcggcgt actgggcctg cggcagcagt tccttgagtt gcgggtcgtt gccgcccgcg
     2041 tccaggtagc gctggatggc gaccgcggcc ttcgggtagt tctcgctgcc gtagtagatg
     2101 ccggccagcg ccttgagcat ggccacgcgg tcttccggcg gcagcagcgg gttggcgagg
     2161 atcgcgtcgt agtcggcggc ggccgcggtg tagtcgttca gcgagtactc ggccgggccg
     2221 cgcaggcgca ggatgaggta cttctcgtac gggttcaggt cggggatcgc ctcgatttcc
     2281 ttgagcttgg ccagcgccac cgcgccgttg cctgccttga gcgcttcctg ggccgtgttg
     2341 aacgcggacc cgaacgcggg acgcacggtg ttcgcaggct gcgatgcggc cgccgcggcg
     2401 gtgtcggcgg cggcggccgg gagcgtggcg gccaggccgg cggcgagggt cagcgccagg
     2461 gcggcgcgcg aatggaaggc tctcatgtct gtcccagggg aaacgctgcg ccgaacgcgg
     2521 aaatggaggt cacgattgca cgcgggcggc gccccgggtg cgagcagaaa agtctgccat
     2581 tgtccccaaa acgcggaacg gggcgcaaac gccgacctgg ggggcgaaaa atcgcccacg
     2641 cacggggatc cgggcgccgg ggagcgccaa gccgggcagg tcgcgtgccc ggccgacgcg
     2701 cttcaggacg cgttcaattc cgcgacgaag tcgtaggtgt cgccacggta gatcgactgc
     2761 gacagctcga tggcgcggcc gtcgcgcagg tagcccaggc gctcgacgag cagcccggcg
     2821 tcgccgacgt cggcgtcgag caggcgtgcc tgctcctcgt tgagcagcag cgcgcgcagg
     2881 cgctgcagcg cgcgcacggg gcggttgccg gccttctcga gcgcgtcgta cagcgagttg
     2941 tcgaccgact ccagcgacgg cagcgccgcc gccgacagcg tggcgtactc gagcgccatc
     3001 ggcgcgtcgt cggcgtagcg caggcggtgg aagcggtaga cgagcgagcc cgggctcagc
     3061 gccagcttca tcgcctcttc cggcgtgacg gtgccgctgg agcgcttgat ccattcgctg
     3121 cgcggcgtgc ggccgcgcgc gcgcatgtct tccgagaacg acgtgagctt ggcgaagttc
     3181 ttgtcgatgc gggtggagac gaagttgccc gagccctggc gctggtcgag gaagccctcg
     3241 tcggccaggc cctggatggc cttgcgcacc gtgatgcgcg agacgccgag gtcggcggcc
     3301 agctggcgct cggaaggcag cgcgtcgtcg ggaccgagca cgcgcttctc gatcgcctcg
     3361 cgcagcgcgc gctgcagttg ttggtaaagc ggctgactgc cgtcggggtc cagcgtgttc
     3421 aggagttgtg ccagcgcgct catgtgcgtg cccttgttcg tgttgtcggg tgctgcggag
     3481 gctccgcccg ccgccggcag ggtaagccgg ggcgccggga gccgcagtct gactagcata
     3541 ccactcaggc accaaagaag cgtcaagcgg accggtcgag cgaaccacgg cggggcggtc
     3601 gcacggaccg tgaatttgcg gcggttccac gggtaaatga ggtgttgccg aaacgcaata
     3661 tgagggtaaa cgcatagagc cccggtctag actggttgta cagtggtact accttgtcgc
     3721 ataccaggtt tctagcccga gggtgcaccg tctgagctcg tggagagacg gcacgaccca
     3781 aaacccaacc ggccgcgttc ccgaagaaat gctgccaccc gaaaagagga gccccatgaa
     3841 gacgaaact
//
LOCUS       DATCRO010000571         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_883958, whole genome shotgun sequence.
ACCESSION   DATCRO010000571 DATCRO010000000
VERSION     DATCRO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883958"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="VIN75_15665"
     CDS             <1..2564
                     /locus_tag="VIN75_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8975656.1"
                     /translation="STVSITVTPASGSPLTYTATTDSNGNWSLNTATATPTSGTMPAA
                     GLPDGTVALKVVSTDTAGNSTTATGSFVEDHTAPGAATIGLKHDAADDTGTSATDNLT
                     NNQKPILTGTAEASSTVTVTVTPSSGGAVTYTATTDATGNWSVNMATATPTSGTLPAS
                     GLVDGAVTLKVVATDAAGNVSVAATSAFTEDHTAPVAAIGLKHDAANDTGVSATDSLT
                     SNPVPVITGTGEKSSTVLVSVTDAGGHTLTYSATTDATGAWSIDLAAQAPTSGTMPRD
                     GLSDGAVSLSVSSTDAAGNTGTATGSFTIDSVDHAATPTVSVSSVGRWLFNEGTGTST
                     ADSYNAYTGTLTNVNTANGGTAPTWITGHNGTAGTALNFDGKGGIVSVPAGDTAALTG
                     TSSLSVWFKTTQTGTNIGWSSPSIIGSESAGDGNDIQWGTLNSSGKIGLGFANSAGVY
                     SAAAVNDGNWHDLQITRTVNANGSSTVSIYIDGALSSSGTIAADSGVPSGIVTNELAG
                     FGYTNGWSSSSGDTTTGDVYYKGALDDAHIYNHVLTADQAKAIYTVENGYETQAVAND
                     GDAIKLVVTDTNTTSLHVTGVENGMTITDGTAAHTITSTGNTQSIDLTGWNLGTLQLT
                     NVGTGSANLQFDAVNTTASGQSHDATSWISVANGTSLIGGTANADTLNASAQTQAMFI
                     SGQAGNDTITGGSGNDRLLGGAGNDTIVGGAGNDLIVGGTGNDTLTGGAGNDVFRWEF
                     GDHGTAGTPNVDTITDFSSVAGNKDVLDLRDLLSGANHDGTQPGNIANYLHFDLSGGD
                     TIIHISSTGGFANGYVASAEDETIILKGVDLTSAGTQNDLQIIHQLLSNGQVHTG"
     gene            complement(2810..3172)
                     /locus_tag="VIN75_15670"
     CDS             complement(2810..3172)
                     /locus_tag="VIN75_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8975657.1"
                     /translation="MHQGSCHCGAVRLTLPSTPEVATKCNCSLCRRIGGPWVYFEFGT
                     VRIEGHPESTEGYVQGDRTLNTIRCRHCGVTTHWEPLPPEPGVRHGVNLGNFDPALIA
                     SVRVRRLDGADTWKYLDE"
     gene            3431..>3881
                     /locus_tag="VIN75_15675"
     CDS             3431..>3881
                     /locus_tag="VIN75_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975658.1"
                     /translation="MPDDAIAMNANLGQDLRVDLVFDGQVLPGFDPAQVQSAVAERFK
                     LDDQRRERMFSGVPYVVKRAMTPEDATRYIDLFAALGARLRPQGSAARGDARPAAPAA
                     MAGPSRDTPSSPTRPPVRPAQASPGSTPGSPSRPAPRAMAAPPGLALV"
BASE COUNT          691 a         1426 c         1301 g          463 t
ORIGIN      
        1 acagcacggt ctccatcacc gtcacgccgg cctccggctc gccgctgacc tacaccgcga
       61 cgaccgacag caacggcaat tggtcgctga acaccgccac ggccacgccc accagcggca
      121 ccatgccggc cgcgggcctg ccggacggca cggtggcgct gaaggtcgtc tccacggaca
      181 ccgcgggcaa cagcacgacg gccaccggct cgttcgtcga ggaccacacc gcgccgggtg
      241 ccgcgacgat cggcctgaag cacgacgccg ccgacgacac cggcacgtcg gccaccgaca
      301 acctgaccaa caaccagaag ccgatcctga ccggcaccgc cgaggcgagc agcacggtga
      361 ccgtcacggt gacgccgtcg tccggcggcg ccgtcacgta caccgcgacg acggacgcga
      421 cgggcaactg gtcggtcaac atggccaccg ccacgccgac ttcgggcacc ctgcccgcca
      481 gcggcctggt ggacggcgcg gtcacgctca aggtcgtcgc gaccgacgcc gcaggcaacg
      541 tgtccgtggc ggccaccagc gcgttcacgg aggaccacac cgcgccggtc gcggccatcg
      601 gcctgaagca tgacgcggcc aacgacacgg gcgtgtccgc cacggacagc ctgacgtcga
      661 acccggtgcc ggtgatcacg ggcacgggcg agaagagcag cacggtgctg gtgtcggtga
      721 ccgacgccgg cggccacacg ctgacctaca gcgcgacgac cgacgccacc ggcgcatgga
      781 gcatcgacct ggcggcgcag gcgccgacct ccggcacgat gccgcgcgac ggcctgtccg
      841 acggcgccgt gagcctgtcg gtcagcagca cggacgccgc cggcaacacc ggcacggcca
      901 cgggctcgtt cacgatcgac tcggtcgacc acgcggccac gccgacggtc agcgtgagct
      961 cggtcgggcg ctggctgttc aacgaaggca cgggcacgtc gacggccgat tcgtacaacg
     1021 cctacaccgg cacgctcacc aacgtgaaca cggccaacgg cggcacggcg cccacctgga
     1081 tcacgggcca caacggcacg gccggcacgg cgctgaactt cgacggcaag ggcggcatcg
     1141 tctcggtgcc ggcgggcgac accgcggcgc tgacgggcac cagctcgctg tcggtgtggt
     1201 tcaagaccac gcagaccggc acgaacatcg gctggagcag tccgtcgatc atcggctcgg
     1261 agagcgccgg cgacggcaac gacatccagt ggggcacgct caacagctcc ggcaagatcg
     1321 gcctgggctt cgccaactcc gccggcgtgt attcggcggc ggcggtcaac gacggcaact
     1381 ggcacgacct gcagatcacc cgcacggtca acgccaacgg atcgagcacg gtatcgatct
     1441 acatcgacgg cgcgctgagc agctcgggca cgatcgcggc cgactccggc gtgccctcgg
     1501 gcatcgtgac caacgagctc gccggcttcg gctacacgaa cggctggtcg tcgagttccg
     1561 gcgacacgac gacgggcgac gtctactaca agggcgcgct cgacgacgcg cacatctaca
     1621 accacgtgct gaccgccgac caggccaagg ccatctacac cgtggagaac ggctacgaga
     1681 cccaggcggt ggccaacgac ggcgacgcga tcaagctggt ggtgaccgac accaacacca
     1741 cgtcgctgca cgtcaccggc gtcgagaacg gcatgaccat caccgacggc accgcggcgc
     1801 acacgatcac cagcacgggc aacacccagt cgatcgacct gaccggctgg aacctgggca
     1861 cgctgcagct gaccaacgtg ggcaccggct cggcgaacct gcagttcgac gcggtcaaca
     1921 cgacggccag cggccagtcg cacgacgcga cgtcgtggat cagcgtggcc aacggcacgt
     1981 cgctgatcgg cggtaccgcc aacgccgaca cgctcaacgc gtccgcccag acgcaggcga
     2041 tgttcatctc gggccaggcg ggcaacgaca cgatcacggg cggctcgggc aacgaccgcc
     2101 tgctgggcgg cgccggcaac gacaccatcg tcggcggcgc aggcaacgac ctgatcgtcg
     2161 gcggcaccgg caacgacacg ctgaccggag gcgcgggcaa cgacgtcttc cgctgggagt
     2221 tcggcgacca cggcacggcg ggcacgccca acgtggacac catcaccgac ttcagctcgg
     2281 tggccggcaa caaggacgtg ctggacctgc gcgacctgct gtccggcgcg aaccacgacg
     2341 gcacgcaacc gggcaacatc gccaactacc tgcacttcga cctgtccggc ggcgacacca
     2401 tcatccacat cagcagcacc ggcggcttcg ccaacggcta cgtggcgtcc gccgaggacg
     2461 agaccatcat cctcaagggc gtggacctga cctccgccgg cacgcagaac gacctgcaga
     2521 tcatccacca gctgctgagc aacgggcagg tgcacaccgg ttgacatcct gcgcggagcg
     2581 aagcggagtc gcaggatcca cctcggcgaa gcagcttcct ggccaccgtg gatcctgcga
     2641 ctgcgcgcag gatgacacct ggttggcgcg aggcggtgac atgccgtcgc gcgactgcga
     2701 ttgcgactgc gcccgcgcca ggatcacacc tggttggcgc aaggcggcca caagggtgtc
     2761 ccgcgttcga ctgctcgctc ctggcgaggt tgccagcctg gcctgagcct cactcgtcca
     2821 ggtatttcca ggtgtcggcc ccgtccaggc ggcgcacgcg caccgacgcg atcagcgccg
     2881 ggtcgaagtt gccgaggttg acgccgtgcc gcacgccggg ctccggcggc agcggctccc
     2941 agtgcgtggt gacgccgcaa tggcggcagc ggatcgtgtt caacgtgcgg tcgccctgga
     3001 cgtagccttc ggtcgactcg gggtggcctt cgatcctcac ggtgccgaac tcgaagtaca
     3061 cccacgggcc gccgatgcga cggcacagcg agcagttgca cttcgtggcg acctcggggg
     3121 tcgagggcag cgtgaggcgg acggcgccgc agtggcatga accttggtgc atcgcgggaa
     3181 ctcctggact gggggacagc gtacctgaac gcgcgccgcg gatcgaacgc gcgctgccga
     3241 cccgggacga gcgccatccg atcacgcgac aggctcgcgg gatgagccag cagcgaggcg
     3301 cgcttgacaa cgtagtcaat cacggacatc gcgccagatg cgatgcgcgg tcaccgatgc
     3361 acgcaaaaaa ccctcgcggc gctgcaaccc ggccgttagc atggcgcccg tgtgccccgg
     3421 gaggatcacc ttgcctgacg acgccatcgc catgaacgcc aacctgggcc aggacctgcg
     3481 ggtcgacctc gtcttcgacg gccaggtgct ccccgggttc gatcccgccc aggtgcagag
     3541 cgcggtcgcc gagcgcttca agctcgacga ccagcggcgc gagcgcatgt tctcgggcgt
     3601 gccctacgtc gtcaagcgcg ccatgacgcc cgaggacgcc acgcgctaca tcgacctgtt
     3661 cgcggcgctc ggggcgcggc tgcgtccgca gggaagcgcg gcgcggggcg atgccaggcc
     3721 tgccgcaccc gcggccatgg ccgggccgtc gcgcgacacg ccttcctcgc cgacacggcc
     3781 gccggtgcgt ccggcgcaag catcgcccgg ctcgacgccc ggctcgccgt cgcgcccggc
     3841 gccgcgtgcg atggccgcgc cgccggggct cgccctggtc c
//
LOCUS       DATCRO010000572         8310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_996382, whole genome shotgun sequence.
ACCESSION   DATCRO010000572 DATCRO010000000
VERSION     DATCRO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996382"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VIN75_15680"
     CDS             complement(<1..488)
                     /locus_tag="VIN75_15680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8975659.1"
                     /translation="MKRSDFRFADPLRVRWAEVDMQKVVFNGHYLMYVDTAMGNWWRA
                     LALPYEDTIAALGGDLFVRKSTLEYLAPARYDDALEVGIRFESAGTSSLRFVAGVFRG
                     EELLVHGQIVYVWTDAATKKPKPAPQALLDVFRAYEAGEAMVDVAVGGWSELGAEAQA
                     IRT"
     gene            complement(485..1399)
                     /locus_tag="VIN75_15685"
     CDS             complement(485..1399)
                     /locus_tag="VIN75_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975660.1"
                     /translation="MAALLERIQRANRPGFETMSPPEARIAYTAAAEVLEPPRAPLPR
                     VEDITLPGAAGVALAARLYAPSMDVLPVLLYLHGGGFTIGNLETHDSLCRQIAKRSGA
                     AVIALDYRLAPEHRFPTAVDDCWAALQHLARPEAAARLGLDTTRLAVGGDSAGGTLAA
                     VCALMARDAGLPLALQVLITPGTIAHADTPSHARFAHGFLLEKQAVDWFFDNYIAVAD
                     RTDWRFAPALAPEHEGVAPACLVIAECDALVDEGVAYGDILRAAGVAVDLEIYRGMTH
                     DFIKMGRMLPEAAQAQQAIADALRKAFE"
     gene            1594..2751
                     /locus_tag="VIN75_15690"
     CDS             1594..2751
                     /locus_tag="VIN75_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY8975661.1"
                     /translation="MARFQYVTQIEIDPGVVRLLPQECKAAGITRPLVVTDAGVRAAG
                     VLDIALAALAGVPHAVFDQTPGNPNEAAVRAATAAYHDNGCDGLVAVGGGSSIDLAKG
                     VAIAATHEGPLANWATITGGADKITSKASPLIAVPTTAGTGSEVARGAILIVDDGRKL
                     GFHNWHLLPRVALLDPDLTMGLPPMLTAATGMDAIAHCLETFMSAKVNPPADGIALDG
                     LRRGWGHIERATRDGRTDGQARWNMMSASMQGALAFQKGLGCVHSLSHSLGGVNPKLH
                     HGTLNAVFLPAVIRFNADCDSMRRNDRLNRMAHAMGLPDGRDHSDGQAVAQAVFDMTA
                     RLGLPTGLAAMGVSEALFDEIIDHALVDHCHATNPRLASRDDYRRMLQESM"
     gene            complement(2778..3212)
                     /locus_tag="VIN75_15695"
     CDS             complement(2778..3212)
                     /locus_tag="VIN75_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY8975662.1"
                     /translation="MPVDAKYKTTATATGGGRDGKTSLADGTLSFQLVVPKELGGPGG
                     DGANPEKLFALGYSACFLGAMRVAAQQLKTKVPDGSTVTATVGIGPRSEGGFGITAEL
                     DVLLPGMADADAKSLVETTHTICPYSNAIKASVPVPTRTRSA"
     gene            3318..3851
                     /locus_tag="VIN75_15700"
     CDS             3318..3851
                     /locus_tag="VIN75_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8975663.1"
                     /translation="MSTLDTDAFADWQARVAAAWKIADTLTPQELVRTVDALCSERPV
                     DDPFAQFERACARDTAGIEDAAEPLYRAALASGALDPYRHGRASIQLGSTLKHLGRLA
                     ESEQLLLAQLERCKAEGPGAVLFDEVRAVLAFTWIAQGRTLEAAALALETLAPHLTRY
                     NRSMAANARALTAPPPP"
     gene            3958..4320
                     /locus_tag="VIN75_15705"
     CDS             3958..4320
                     /locus_tag="VIN75_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975664.1"
                     /translation="MNKALNTLATALALVGAIGLAQAQGTGGSGGSGTSGSTGAPGNA
                     GTPSGGTSPTGGSNSGTSTGKSSPTANTGNSADTGAGVRSSPETSGTNTATSSHRKHS
                     HAKRAKRSTERASEPSGS"
     gene            complement(4378..4956)
                     /locus_tag="VIN75_15710"
     CDS             complement(4378..4956)
                     /locus_tag="VIN75_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY8975665.1"
                     /translation="MTKQLKDMKVAIVVDDLFEQAELAEPRKALEAAGVTVHIIAPKG
                     PTVTGMKHHDKGDSFPVSVSLDQAKPEDYDGLVLPGGVVNADALRMNALAQAFARSID
                     QAGKPLAVVCHGPWLLISADLVSGRTLTSWPTLQDDLRNAGATWVDQEVVSDGNWTSS
                     RKPADLPAFNRAFIEALQREPARQPETMPIAD"
     gene            complement(5147..6445)
                     /gene="aceA"
                     /locus_tag="VIN75_15715"
     CDS             complement(5147..6445)
                     /gene="aceA"
                     /locus_tag="VIN75_15715"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY8975666.1"
                     /translation="MTQLTRDQQIAALEKDWAENPRWKGVRRTYSAADVVRLRGSLQI
                     DHTLARRGSEKLWNLINTEPFINALGALTGNQAMQQVKAGLKAIYLSGWQVAGDANSN
                     GEMYPDQSLYSVDSVPKVVKKINATFKRADEIQWSEGKNDIDFFAPIVADAEAGFGGV
                     LNAFELMKAMIEAGASGVHFEDQLAAAKKCGHMGGKVLVPTREAVSKLSAARLASDVM
                     GVPTVLLARTDAEAGDLVTSDIDDNDKPFCTGERTVEGFFRTRNGLEQAISRGLAYAP
                     YADMIWCETGKPDLAFAKAFADAIHAKFPGKLLAYNCSPSFNWKKNLDDATIAKFQRE
                     LGAMGYKFQFITLAGFHALNYSMFNLAYGYARNNMSAFVELQESEFAAAEKGFTAVKH
                     QREVGTGYFDAVTTTIEREASTGALKGSTEDEQFFDKKHA"
     gene            7060..7584
                     /locus_tag="VIN75_15720"
     CDS             7060..7584
                     /locus_tag="VIN75_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8975667.1"
                     /translation="MIETRANDTLPMLDRLDATHDQMRYWLGELQTLVNHMEDRGWDT
                     WARDHARSVIDFFSHTARQHHLDVDRQIFPLVSGRGDASIDISIDRLRQDQGWLEENW
                     REVLAQLQPVAEGFGGYDVDMLRHAADVYAALLLEHLALEQGSVFPAARASADKQMKA
                     SETRRGGAEIGAAA"
     gene            complement(7631..8116)
                     /locus_tag="VIN75_15725"
     CDS             complement(7631..8116)
                     /locus_tag="VIN75_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8975668.1"
                     /translation="MRQIPKSLQLDAIDRSILSELQNDGRLPNVELAARVHLSPSACL
                     RRVKQLEDSGVIAGYVALLNPKLVGQSGVSFTIINLATMSNALLEAFEQAVVEAPEIL
                     DCYYVAGSNDYLIRFAYRDAEDLERFHTEVLMRLPGVERSNSMLVLRTVKKTTALPVG
                     E"
BASE COUNT         1281 a         2925 c         2841 g         1263 t
ORIGIN      
        1 gtgcggatcg cctgcgcctc ggcgcccagc tcgctccagc cgccgaccgc gacgtcgacc
       61 atggcctcgc cggcctcgta ggcgcggaat acgtcgagca gcgcctgcgg ggccggcttg
      121 ggcttcttcg tggccgcgtc cgtccacacg tagacgatct ggccgtgcac cagcagttcc
      181 tcgccgcgga acacgcccgc gacaaagcgc agcgagctgg tgccggccga ctcgaagcgg
      241 atgccgacct ccagcgcgtc gtcgtagcgc gccggcgcca ggtattccag cgtcgacttg
      301 cgcacgaaca ggtcgccccc gagcgcggcg atcgtgtcct cgtacggcag cgccagcgcg
      361 cgccaccagt tgcccatcgc ggtgtcgacg tacatcaggt agtggccgtt gaagaccacc
      421 ttctgcatgt ccacctcggc ccagcgcacg cgcagcgggt cggcgaatcg gaagtcggaa
      481 cgtttcattc gaaggctttc ctcagggcgt cggcgatggc ctgctgggcc tgcgccgcct
      541 cgggcagcat gcggcccatc ttgatgaagt cgtgcgtcat gccgcggtag atctccaggt
      601 cgaccgcgac gccggcggcg cgcaggatgt cgccgtacgc gacgccctcg tcgaccagcg
      661 cgtcgcattc ggcgatgacc aggcatgccg gcgcgacgcc ctcgtgctcg ggcgcgagcg
      721 cgggcgcgaa gcgccagtcg gtgcggtcgg cgacggcgat gtagttgtcg aagaaccagt
      781 cgacggcctg cttctccagc aggaagccgt gggcaaagcg ggcgtgcgac ggggtgtcgg
      841 cgtgcgcgat ggtgcccggg gtgatgagca cctgcagcgc gagaggcagg ccggcgtcgc
      901 gcgccatcaa cgcacacacg gcggccagcg tgccgccggc gctgtcgccg cccacggcca
      961 ggcgcgtggt gtccaggccc aggcgcgctg cggcctccgg ccgcgcgagg tgctgcaacg
     1021 cggcccagca gtcgtcgacg gccgtcggga agcggtgttc cggcgcgagg cggtagtcca
     1081 gcgcgatcac cgcggcgccg ctgcgtttgg caatctggcg gcacaggctg tcgtgcgtct
     1141 ccaagttgcc gatcgtgaag ccgccgccgt gcaggtacag cagcacgggc aggacgtcca
     1201 tcgacggcgc gtacaggcgc gcggcgagcg cgacgccggc ggcgccgggc agcgtgatgt
     1261 cctccacccg cggcagcggc gcgcgcggcg gctccagcac ctcggccgcg gcggtgtagg
     1321 cgatgcgcgc ctcgggcggc gacatcgtct cgaagccggg acggttggcg cgctggatgc
     1381 gttcgagcag cgcggccatg cgcggggtca ggagatcttg ggcggacacg ggagagcggt
     1441 gacgggcggc acgaccggca agcatacggc ctcgcgcgcg gccgtgccgc cggttcagca
     1501 tccgaaccgt gccggcgcca tgcgacgcgg ccgcgacagc agcgtcccgc gccgcgtcct
     1561 acactgcgtc gtcgcctcca gttccagacc gccatggccc gcttccaata cgtcacgcag
     1621 atcgagatcg acccgggcgt cgtgcgcctg ctcccgcagg aatgcaaggc tgccggcatc
     1681 accaggccgc tggtggtgac cgacgccggc gtgcgcgccg cgggcgtgct ggacatcgcg
     1741 ctggccgcgc tcgccggcgt gccgcacgcc gtgttcgacc agacgcccgg caacccgaac
     1801 gaggccgccg tgcgcgcggc cacggcggcc taccatgaca acggctgcga cggcctcgtc
     1861 gccgtgggcg gcggctccag catcgacctg gccaagggcg tcgccatcgc cgccacgcac
     1921 gagggcccgc tcgcgaactg ggcgacgatc accggcggcg ccgacaagat cacgagcaag
     1981 gcctcgccgc tgatcgccgt gcccaccacc gcgggcaccg gcagcgaggt ggcgcgcggc
     2041 gccatcctga tcgtcgacga cggccgcaag ctgggcttcc acaactggca tttgctgccc
     2101 cgcgtcgcgc tgctcgaccc ggacctgacg atgggcctgc cgccgatgct gaccgccgcg
     2161 accggcatgg acgcgatcgc gcactgcctg gagaccttca tgtcggccaa ggtgaatccg
     2221 cccgccgacg gcatcgcgct ggacggcctg cgccgcggct ggggccacat cgagcgcgcg
     2281 acgcgcgacg gccgcaccga cgggcaggcc cgctggaaca tgatgagcgc gagcatgcag
     2341 ggcgcgctcg cgttccagaa ggggctgggc tgcgtgcaca gcctcagcca cagcctgggc
     2401 ggcgtgaacc cgaagctgca ccatggcacg ctcaacgccg tgttcctgcc ggccgtcatc
     2461 cgcttcaacg cggactgcga ttcgatgcgc cggaacgacc ggctgaaccg catggcccac
     2521 gcgatgggcc tgcccgacgg ccgcgaccat tcggacggcc aggccgtcgc ccaggccgtg
     2581 ttcgacatga ccgcgcgcct gggcctgccc accggcctgg cggccatggg cgtgagcgaa
     2641 gcgctgttcg acgagatcat cgaccacgcg ctggtcgacc actgccacgc gacgaacccg
     2701 cgcctggcca gccgcgacga ctaccgccgg atgctgcagg aatcgatgta ggcctcgcgt
     2761 cccggcgcgg gcgggcgtca cgcgctgcgc gtgcgcgtgg gcaccggcac gctggccttg
     2821 atcgcgttcg agtacgggca gatcgtgtgc gtcgtctcga ccagcgactt cgcgtccgcg
     2881 tcggccatgc cgggcagcag gacgtcgagc tcggcggtga tgccgaagcc gccttccgag
     2941 cgcgggccga tgccgacggt ggccgtgacg gtcgatccat cgggcacctt ggtcttgagc
     3001 tgctgcgcgg ccacgcgcat cgcgccgagg aagcaggcgg agtagcctag cgcgaacagc
     3061 ttctccgggt tggcgccgtc gccgccgggg ccgccgagct ccttgggcac gacgagctgg
     3121 aacgacagcg tgccatcggc cagcgacgtc ttgccgtcgc ggccgccgcc ggtcgcggtg
     3181 gcggtggtct tgtacttcgc gtccacgggc atggtcgggt ctccttgaaa ggtgtggcgg
     3241 acatgctagc cagcgcttcg cgcacgcgtg cgaccgcctc gaacgaccct gcccgcgacg
     3301 cgaactaaca tcgccgcatg agcaccctcg ataccgacgc cttcgccgac tggcaggccc
     3361 gcgtcgccgc cgcctggaag atcgccgaca ccctgacgcc gcaggaactc gtgcgcaccg
     3421 tcgacgcgct gtgcagcgag cggcccgtcg acgacccgtt cgcccagttc gagcgcgcct
     3481 gcgcccgcga tacggccggc atcgaggacg cggccgagcc gctgtaccgc gccgcgctgg
     3541 ccagcggcgc gctcgacccc taccgccacg gccgcgcgag catccagctg ggcagcacgc
     3601 tcaagcacct gggccgcctg gccgagagcg aacagctgct gctcgcgcag ctcgagcgct
     3661 gcaaggccga aggcccgggc gccgtgctgt tcgacgaggt gcgcgccgtg ctcgcgttca
     3721 cgtggatcgc gcagggccgc acgctcgagg ccgcggcgct cgcgctcgag acgctggcac
     3781 cgcacctgac gcgctacaac cggtcgatgg cggcgaacgc gcgggcgctg accgcgccgc
     3841 cgcctccgtg acgccggaca aacgttcggt caagcaccgc gctcggcggg gcattcgcat
     3901 tgccgccccc ggtgtcgcgc ctcgatggtc gagacgcgca cacacagggg aattgcaatg
     3961 aacaaggctc tcaacacgtt ggcgaccgcg ctcgcgctgg tcggggcgat cggtctcgca
     4021 caggcgcagg gcaccggcgg ctcgggcggg tccggcacgt cgggcagcac cggcgcgccc
     4081 ggcaacgcgg gcacgccgtc gggcggcacc agcccgacgg gcggcagcaa ctccggcacg
     4141 tcgaccggta aatccagccc gaccgccaac acgggcaatt cggccgacac gggtgccggc
     4201 gtgcgcagct cgccggagac cagcggcacc aacaccgcca cctcctcgca caggaagcac
     4261 tcgcacgcca agcgcgcgaa gcgctccacc gagcgggcca gcgagccgtc gggcagctga
     4321 tcccaccgca ggaagaccgc ggccgcaggc cggccgcggc gcccggcggc gcgccgctca
     4381 atcggcgatc ggcatcgtct ccggctgccg cgccggctcg cgctgcagcg cctcgatgaa
     4441 cgcacggttg aatgccggca ggtcggcggg cttgcggctc gacgtccagt tgccgtcgct
     4501 caccacttcc tggtcgaccc aggtcgcgcc ggcattgcgc aggtcgtcct gcagcgtcgg
     4561 ccagctcgtc agcgtgcggc cggacacgag gtcggcggag atcagcagcc agggcccgtg
     4621 gcacacgacc gccagcggct tgcccgcctg gtcgatggac ctcgcgaagg cctgcgcgag
     4681 cgcgttcatg cgcagcgcat cggcgttgac cacgccgccc ggcagcacga ggccgtcgta
     4741 gtcctccggc ttggcctggt cgagcgacac gctcaccggg aaactgtcgc ccttgtcgtg
     4801 gtgcttcatg ccggtcaccg tcggaccctt gggcgcgatg atgtgcacgg tgacgccggc
     4861 ggcctcgagc gccttgcgcg gctcggccag ctcggcctgc tcgaacaggt cgtcgaccac
     4921 gatggcgacc ttcatgtcct tgagttgctt ggtcatggga atcctctcgt ccgttggaac
     4981 acaccagggt gcggattcct gcgggcggca gtccccatgc ccggccgagg acccggtgcg
     5041 cacggacgaa aaaaagccca cccggtgctc aagtcgggtg ggctccatcg tcgctgccga
     5101 tccggcgtcc gcccctcgcg gccgccggtc ggcgcgacgt cacgcgtcaa gcgtgcttct
     5161 tgtcgaagaa ctgctcgtct tcggtggaac ccttcagcgc gccggtggag gcttcgcgct
     5221 cgatcgtcgt cgtcacggcg tcgaagtaac ccgtgcccac ttcgcgctgg tgcttgacgg
     5281 cggtgaagcc cttctcggca gcggcgaatt ccgattcctg cagctcgacg aacgcgctca
     5341 tgttgttgcg cgcgtagccg taggccaggt tgaacatcga gtagttcagg gcatggaagc
     5401 cggccagcgt gatgaactgg aacttgtagc ccatcgcgcc cagctcgcgc tggaacttgg
     5461 cgatggtcgc gtcgtcgagg ttcttcttcc agttgaacga gggcgagcag ttgtacgcga
     5521 gcagcttgcc cgggaacttg gcgtggatcg cgtcggcgaa cgccttggcg aacgcgaggt
     5581 cgggcttgcc cgtctcgcac cagatcatgt cggcgtacgg cgcgtaggcg aggccgcggc
     5641 tgatggcctg ctccaggccg ttgcgggtgc ggaagaagcc ttccacggta cgctcgccgg
     5701 tgcagaacgg cttgtcgttg tcgtcgatgt cgctggtgac caggtcgccg gcttcggcgt
     5761 cggtgcgtgc gagcagcacg gtgggcacgc ccatcacgtc ggaggccagg cgcgcggccg
     5821 acagcttgga cacggcctca cgcgtgggca ccagcacctt gccacccatg tggccgcact
     5881 tcttggcggc ggccagctgg tcctcgaagt gcacgcccga ggcgccggct tcgatcatgg
     5941 ccttcatcag ctcgaacgcg ttcagcacgc cgccgaagcc ggcttcggcg tcggccacga
     6001 tcggcgcgaa gaagtcgatg tcgttcttgc cttccgacca ctggatctcg tcggcgcgct
     6061 tgaacgtggc gttgatcttc ttgacgacct tgggcaccga gtccaccgag tacagcgact
     6121 ggtcggggta catctcgccg ttgctgttgg cgtcgccggc gacctgccag cccgacaggt
     6181 agatggcctt caggccggcc ttgacctgct gcatcgcctg gttgcccgtc agcgcgccca
     6241 gcgcgttgat gaagggctcg gtgttgatca ggttccacag cttctcggag ccgcggcgcg
     6301 cgagcgtgtg gtcgatctgc agcgagccgc gcaggcgcac gacatcggcc gctgagtacg
     6361 tgcgcctgac gcccttccag cgcgggttct cggcccagtc cttctcgagg gcggcgatct
     6421 gctggtcacg ggtgagttgg gtcatggtgg cttccttggg ggttctgtga agggtggaag
     6481 aagaggtgtt tcgttgggat caagcgtatt cctgttgcag gagaaatgga agacatgtgt
     6541 cttatataag actaaagaag tagtccttat taaacaacga cttagcatcg cattgtcgca
     6601 atacggaaag ccatttctca taacgagaaa ttttgctgcg acgcaccagc caccaattcc
     6661 gcattgcgga acgttctttc cgatgcgaaa ttgacgtttt ccgaagcgtc cgttcgcgca
     6721 tcgacaagcc cgcgccggac gcgtccgcgg cggtgcggac ctcagcattc ccgcggctcg
     6781 ctcgcaccgg ttcgcgacgt gcaggatcga cgcagcgagg cctgcccgcg aacgcgccgg
     6841 gtaccgcgaa cgagtgaccc cggacgacat cggggacacg gtcgagcacg cgcgtcggac
     6901 gctcgccctg cacggcgagc acgacacgga accgaccctg tcaagaggcg gcaacgcgct
     6961 gctgccgtta catcttttcc ggggaaaaga ttcttgaaaa aggcgtcgcc ccatgcgatg
     7021 ctgaagtcaa ccaaccgacg tccatttcga ggagatgccg tgatcgaaac ccgcgccaac
     7081 gacaccctgc cgatgctcga ccgcctcgat gccacgcacg accagatgcg ctactggctc
     7141 ggcgagctgc agacgctggt gaaccacatg gaagaccgcg gctgggatac ctgggcgcgc
     7201 gaccacgccc gctcggtgat cgacttcttt tcgcacacgg cgcgccagca tcacctcgat
     7261 gtcgatcgcc agatcttccc gctcgtctcg ggccgcggcg acgcctcgat cgacatcagc
     7321 atcgaccgcc tgcgccagga ccagggctgg ctcgaggaga actggcgcga ggtgctcgcg
     7381 cagctgcagc cggtggccga aggcttcggc ggctacgacg tcgacatgct gcgccacgcg
     7441 gccgatgtct acgccgcgct gctcctggag cacctggcgc tcgagcaggg cagcgtgttc
     7501 ccggccgcgc gcgcgtccgc cgacaagcag atgaaggcct ccgagacgcg ccgcggcggc
     7561 gccgagatcg gcgcggccgc ctgagcccgt ccaccagcgg gccggcggcg ccggcctgcc
     7621 cttggcgagg ctactcgccc accggcagcg ccgtcgtctt cttgaccgtg cgcagcacca
     7681 gcatcgaatt cgaacgctcc acgcccggca gccgcatcag cacctcggtg tggaagcgtt
     7741 ccaggtcctc ggcgtcgcgg tacgcgaagc ggatcaggta gtcgttcgac ccggccacgt
     7801 aatagcagtc caggatctcc ggcgcctcga cgacggcctg ctcgaacgcc tccagcagcg
     7861 cattgctcat cgtcgccagg ttgatgatcg tgaagctgac gccgctctgg cccaccagct
     7921 tcggattgag cagcgccacg taacccgcga tgacccccga gtcctccagc tgcttcaccc
     7981 gccgcaggca ggccgacggc gacaggtgca cgcgcgccgc cagctccacg ttgggcagtc
     8041 gcccgtcgtt ctgcagttcg ctcaaaatgc tgcgatcgat cgcatccaat tgcagggatt
     8101 tcggtatttg gcgcatgaga tgcgaatgga ttgaacttcg ctcgcatgat attgcgtttt
     8161 ccggggtttg ccctgcgtca tcctgcgcac acattgcgcg ccctcctcgc tacagtcgag
     8221 tccatcgatc gaacgaagtc gatgacagcg acgacgagcg tccgacctga atcagaaaga
     8281 cccgcatggc caccgatctc gacgcctact
//
LOCUS       DATCRO010000573        10614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_895145, whole genome shotgun sequence.
ACCESSION   DATCRO010000573 DATCRO010000000
VERSION     DATCRO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895145"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..2353)
                     /locus_tag="VIN75_15730"
     CDS             complement(173..2353)
                     /locus_tag="VIN75_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975669.1"
                     /translation="MRPADVLPPARDRVLLSATLAVILLTWAAVAFACWRGRQDAIDD
                     WRYFLANLSEMAAQHADQTLAAADAVLGRVVDDVKNVAPVDGADLARKTGTRETYELL
                     RRRQGDLPQIDVISIVGSDGRLISFSRSFPTPVINLADRDYLQAHLADDSLALFLSTP
                     VKNRGTGRWTFYLARKLRGTDGRMVGLAIVGIECEYFQHFYESIDSAREGVTVLLLRR
                     DGLLLARQPPIDEFVGRSFAGGPVFQLLARAGANVPRTAFIDGPRVVDRLDARPRLVA
                     PTLSRAYPTVVALVATSDLILAHWRRTSIALVAVTLAFDAVLAAFAIGLARLLRRRRE
                     ILSQLKGALAAADAASQAKSSFLANMSHEIRTPMNGILGMTELLLQTPLDARQRELAA
                     AAHASGQTMLHVLNDVLDVSKIEAGKVALERVDFDLRQLLDDELALYRATAAATGITL
                     AGEFDPALPARVIGDPTRLRQVLTNLLNNAVKFTERGGDVRLGARCIGGDATSHRLAF
                     EVRDTGIGISEAAQRRLFEPFTQADESTTRRFGGTGLGLAISKELVGLMGGTLSLESR
                     PGAGSTFRIELPLAASAAPAQAAPPAAAAEGADRRLAGLHLLVAEDNPVNLQVVVAML
                     EGMGARVTCAVDGAQAVAQCRDGSFDALLLDIQMPGMDGYEAARRIRAEGARRMPIIA
                     VTANAMPTDRTLALAAGMNDHLAKPLTRDALAVMVLKWARPVDA"
     gene            2487..3482
                     /locus_tag="VIN75_15735"
     CDS             2487..3482
                     /locus_tag="VIN75_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8975670.1"
                     /translation="MGANDAFLQDAYSRTVSQVVERVGPSVAAVRLHGAGGSDSSLGG
                     GSGFLFTPDGYVLTNSHVVRAGKPPAAARPELRHQVSLADGREFDARWVGDDPDTDLA
                     VLSIDGLSRGALGHAPLGRSAGLKRGQIAIAIGNPLGFEHTVTAGIVSALGRSMRTST
                     GRLIPDVIQTDAALNPGNSGGPLLDSNGEVIGVNTAIIRGAQAICFAVAADIAHWVIP
                     QLLQHGRVRRGYLGVAGQTQELHRRVVLTYELPDSTGVRVMSVEPDSPAARVGVREGD
                     LIVGLDGVAIPSVDALHQALDASRVNRDCVLKIIRGVRQPVPMYLTVRPLEVLRA"
     gene            3532..3801
                     /locus_tag="VIN75_15740"
     CDS             3532..3801
                     /locus_tag="VIN75_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HEY8975671.1"
                     /translation="MGIVKISDLMHDNLRDASDALSRSINSQAEHWLRVGMLAELHPE
                     LNYGDICQLMLRDARAGGGGLGFVKPEVAAAASATAGKAIRRAAR"
     gene            3798..4592
                     /gene="map"
                     /locus_tag="VIN75_15745"
     CDS             3798..4592
                     /gene="map"
                     /locus_tag="VIN75_15745"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8975672.1"
                     /translation="MRRGANLVKSPEEIALARAAGALAADVLRMIAPHVKAGVTTDEL
                     DRLCHDHIVGVQKAVPANVGYHGYPKTTCISVNHVVCHGIPDGKALKDGDILNIDVAV
                     IKDGWYGDTSRMYYVGTPSVHARRLVDTTYEAMRAGIRSVRPGATLGDVGHAIQQVAE
                     KAGFSIVRDYCGHGIGRIYHDDPQVCHFGRRGEGMRLQEGMVFTIEPMVNAGKPGTRE
                     LADGWTVVTRDRSLSAQWEHMVAVTHDGFEVLTPWPDGTGAYAAIG"
     gene            4758..6947
                     /locus_tag="VIN75_15750"
     CDS             4758..6947
                     /locus_tag="VIN75_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8975673.1"
                     /translation="MSRSNLALRMICAALAGASTLAQAAATTLPVAPVKAVTDTYFGT
                     TVTDTYRYMEDLSAPEVQQWAKAQADHAREALDAIPGRAPLLARIRELDDSVKERVQS
                     VALAGGGLVFYEKRGADDNQLKLYVRQGYKGAERLLVDPDALAKADGTPHAIEFFQAS
                     NNGRYVAYAISAGGSEEAVIHVMDVATGKEVMAPIDRAHYSYVAWLPDDSGFFYMRLQ
                     ALAKGAPETDKYQRATAWFHRMKGGAADVAVLTAGTDTHLKIAAAEMPVVMPVVGTRW
                     TVAIPANGVENEIDLYAAPQGKALDPKVKWRKLVGRESQVTGFAIHGDDLYLLTHLNA
                     SRYKVIRTSMSHPDVAMADVVVAPGEEVVDRIAAAKDGLYIQSRDATVGKLYRLAYAK
                     DAQPEPVQLPASGAVTIVDNDLTRAGVVVSIDSWTHDEAFYSVAGNGGPVADTGLQVA
                     GAFGAPADLVAEEVKVKSYDGLEIPLSIVHRKDMKLDGGNPLILEAYGAYGVVDTPGY
                     APRQLAWYELGGVYATCHVRGGGIYGENWHLAGKQLSKPNSWKDAIACGEWLVKNGYT
                     SSARMALNGGSAGGITVGRAITERPDLFAVAVPEVGVLNAVRAETSANGVPNIPEFGS
                     VKDKQQFNGLLEMDAFHHVQDGVNYPATLLTTGINDPRVPAWESMKMAARMQAATASG
                     KPVLLRIQYDGGHGIGSTKKQRQELAADRWSFMLWQFGDARFQPATK"
     gene            complement(6995..7966)
                     /locus_tag="VIN75_15755"
     CDS             complement(6995..7966)
                     /locus_tag="VIN75_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8975674.1"
                     /translation="MIDALHSFLAVASSSSFSQVAKAQAVAVSSVTRKIDWLEAEIGT
                     RLFHRSSRRVMLTDAGEQFLPRARNILAELAQARDALASIDADPRGTMTVTAPTIFGR
                     RHLAQAVASFLERYPMIEVDLHVSDALVDLAEQRVDVAIRIGMLPDSDLVATRLATLR
                     PLVVASPAYLARHGRPARPEDLVRHECITGPAPTAGSPSWRFAGVNRGAALPVRGKLR
                     IDDKDCALQAALAGIGIAFLASWVVSEDIVAGRLVSLFPDMKPADPASLPAIHAVRMP
                     GRSSEAKARLFIAHLREAIGETPYWDVALDRPRTRARGGARVAPARA"
     gene            8095..9078
                     /locus_tag="VIN75_15760"
     CDS             8095..9078
                     /locus_tag="VIN75_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8975675.1"
                     /translation="MTRFQRLFQLSVLSAAIALGGAALLTAAPAAHAAAPQVRTQAPG
                     FHRAILGDFEITALSDGTVDLDVTKLLHEPAARTNAALARDFVKTPLETSVNAFLVNT
                     GTKLVLIDTGAGTLFGPTLGKLVDSIKAAGYTPDQVDDIFITHFHPDHVGGLSANGER
                     VFPNATLHADKAESDFWLSQANLDKAPADMKAFFQGAMGSVNPYVAAGKYQPFEADGE
                     PVPGIRTHATHGHTAGHTSYVVESKGQRLVVIGDLIHVAAVQLPNPGVTIAFDSDAKA
                     AAAARDAVFRQAAKDGSLVAAAHLQFPGLGHLRAAGKSWQWVPVNYTTELK"
     gene            complement(9101..9850)
                     /locus_tag="VIN75_15765"
     CDS             complement(9101..9850)
                     /locus_tag="VIN75_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8975676.1"
                     /translation="MTARALIAEDEPLLAAELRDELAQLWPELEICAVVHDGHDALRQ
                     VQARKPDVLFLDVQMPGLDGVALARLAGPRPHVVFVTAFDRYAVSAFDEGAVDYLLKP
                     VETERLARAVQRLKERLADAAPPAAPVPAPAASDAGAPSSPLRWISVLQGRDVKLIEV
                     DDVCYFRADHKYVAVVTTDGEALITTPLKDLLPRLDPALFWQVHRSTVVNMRAVHSVA
                     RTPTGRMLLRLKQRPERLDVSASYTHLFKQF"
     gene            complement(9825..>10614)
                     /locus_tag="VIN75_15770"
     CDS             complement(9825..>10614)
                     /locus_tag="VIN75_15770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8975677.1"
                     /translation="IWLLGNLIVLALGGIPGTWNVMDDLSLALGRMLSLGTLTFVHAT
                     WRGEQRALAALRRGEIEQAARQRAVETARLLALQARVEPEFLFDTLGRVNRLARRSTA
                     AADRLLMALTHLLRALQGGGAQAASSLQRELSLVSAYARTSARSGLRPPRLLMSASAQ
                     ARATALAPGVLLPLLRDLARHGPSRWRLDAHLEADALAIAVVAEPADAAARHALAGVD
                     QPSLLARLRGVHGPGARLAIAERDAACGLHLHLPVPDDRQGPDR"
BASE COUNT         1616 a         3744 c         3843 g         1411 t
ORIGIN      
        1 ggcccgcggc aaggccaaga tgagtgccct cggcgccgcc atgcataagc tcgcgcaatt
       61 gtgcttcggc gtcgttcaca caggccagcc ctaccgcgct gattggcctg tcaaggcttg
      121 acgcccgaga cggtatctac gaataccctc cctagggaag gaaatcccgc cgtcacgcgt
      181 ccacgggccg cgcccacttg agcaccatca ccgccagcgc atcgcgcgtc agcggcttgg
      241 cgaggtggtc gttcatgccg gcggccagcg ccagcgtgcg gtcggtgggc atcgcgttgg
      301 ccgtcacggc gatgatgggc atgcgccgcg cgccctcggc gcggatgcgg cgggcggcct
      361 cgtagccgtc catgccgggc atctggatgt ccagcagcag tgcgtcgaaa ctgccgtcgc
      421 ggcattgcgc cacggcctgc gcgccgtcga cggcgcaggt gacgcgcgcg cccatgcctt
      481 cgagcatcgc gacgaccacc tgcaggttga ccggattgtc ctcggccacc agcaggtgca
      541 gcccggccag ccggcggtcg gcgccttcgg ctgccgcggc gggaggcgcg gcctgtgccg
      601 gagcggccga ggccgcgagc ggcagctcga tgcggaacgt cgagcccgcg ccggggcggc
      661 tctcgagcga cagcgtgccg cccatcagtc cgaccagttc cttgctgatc gccagtccca
      721 gcccggtgcc gccgaagcgt cgcgtcgtcg actcgtcggc ctgcgtgaac ggctcgaaca
      781 gccggcgctg cgcggcctcg ctgatgccga tgccggtgtc gcgcacctcg aaggccagcc
      841 ggtgcgacgt cgcgtcgccg ccgatgcatc gggcgcccag ccgcacgtcg ccgccgcgct
      901 cggtgaattt gacggcgttg ttcaggaggt tcgtcaggac ctgccgcagc cgcgtcggat
      961 cgccgatgac gcgcgccggc agcgcggggt cgaactcgcc ggccagcgtg atgcccgtgg
     1021 ctgcggccgt cgcgcggtac agcgccagtt cgtcgtcgag cagctggcgc aggtcgaagt
     1081 cgacccgctc gagcgcgacc ttgccggcct cgatcttgga cacgtcgagc acgtcgttga
     1141 gcacgtgcag catcgtctgg cccgacgcgt gcgccgccgc ggccagctcc cgctggcgcg
     1201 cgtccagcgg cgtctgcagc agcagctcgg tcatgcccag gatgccgttc atcggcgtgc
     1261 ggatctcgtg gctcatgttg gccaggaacg aggacttggc ctggctcgcc gcatcggccg
     1321 cggcgagcgc gcccttgagc tgcgacagga tctcgcgccg gcgccgcagc aggcgggcca
     1381 gcccgatcgc gaaggcggcc agcacggcgt cgaacgccag ggtgaccgcg acgagtgcga
     1441 tgctcgtccg acgccagtgc gccaggatca ggtcgctcgt ggccacgagg gcgacgacgg
     1501 tcggataggc ccgcgacagc gtcggcgcga ccagcctcgg gcgtgcgtcg agtcgatcga
     1561 cgacgcgcgg cccgtcgatg aacgccgtgc gcggaacgtt ggcgccggcc cgcgccagga
     1621 gctggaacac cgggccgccg gcgaagctgc gaccgacgaa ttcgtcgatc ggcggctggc
     1681 gcgcgagcag caggccgtcc cggcgcagca gcaggacggt gacgccctcg cgggcggagt
     1741 cgatcgactc gtagaagtgc tggaagtact cgcactcgat gcccacgatg gccaggccga
     1801 ccatgcggcc gtcggtgccg cgcagcttgc gcgccaggta gaacgtccag cgcccggtgc
     1861 cgcggttctt cacgggggtg gacaggaaca gcgcgaggct gtcgtcggcg aggtgggcct
     1921 gcaggtagtc gcgatcggcc aggttgatca ccggcgtcgg gaagctgcgc gagaaactga
     1981 tcagccggcc gtcgctgccc acgatggaga tgacgtcgat ctgcggcagg tcgccctggc
     2041 gccggcgcag cagctcgtag gtctcgcgcg tgccggtctt gcgggcgagg tccgcgccat
     2101 cgacgggcgc cacgttcttg acgtcatcca ccacccgccc gagcacggca tcggcggcgg
     2161 ccagcgtctg gtcggcgtgc tgggccgcca tctccgacag gttggccagg aagtagcgcc
     2221 agtcgtcgat ggcatcctgg cggccgcgcc agcacgcgaa cgcgaccgcg gcccaggtca
     2281 gcaggatcac cgccagggtg gcgctcagca gcacacggtc gcgggcgggc gggaggacgt
     2341 cggcgggacg catcgggcga ttgtggcgcc gtggcggccg gcgtggggcg gcaagtgcgg
     2401 tcggtgccgg gggggggccg gcacgccgcc ccggcccttg aaatcgctcc gttcgccctg
     2461 cagttccggc ctcgaaggag attcccatgg gtgccaacga tgccttcctg caggacgcct
     2521 actcgcgcac ggtcagccag gtggtggagc gggtcgggcc cagcgtggcc gcggtgcggc
     2581 tgcacggcgc gggcggctcg gactccagcc tgggcggcgg ctcgggcttc ctgttcacgc
     2641 ccgacggcta cgtgctgacg aactcgcacg tggtgcgcgc gggcaagccg ccggcggccg
     2701 cgcggcccga gctgcgccac caggtgtcgc tggcggacgg ccgcgagttc gacgcgcgct
     2761 gggtcggcga cgaccccgac accgacctcg cggtgctgag catcgacggc ctgtcgcgcg
     2821 gcgcgctggg ccatgcgccg ctgggccgct cggcgggcct gaagcgcggc cagatcgcga
     2881 tcgccatcgg caatccgctg ggcttcgagc acacggtcac ggccggcatc gtcagcgcgc
     2941 tgggacgcag catgcgcacg agcaccgggc gcctcatccc cgatgtcatc cagaccgacg
     3001 ccgcgctgaa tcccggcaac tcgggcgggc cgctgctcga ttcgaacggc gaggtcatcg
     3061 gcgtcaacac cgcgatcatc cgcggcgcgc aggcgatctg ctttgccgtg gcggccgaca
     3121 tcgcgcactg ggtcatcccg cagttgctgc agcacggacg cgtgcgccgc ggctacctgg
     3181 gcgtggccgg ccagacgcag gaactgcacc ggcgcgtcgt cctcacctac gagctgcccg
     3241 attccaccgg cgtgcgcgtg atgagcgtcg agcccgacag ccccgccgcg cgcgtgggcg
     3301 tgcgcgaggg cgacctgatc gtgggcctgg acggcgtggc gatcccgagc gtcgatgcgt
     3361 tgcaccaggc gctcgacgcg agccgcgtca accgcgactg cgtgctcaag atcatccgcg
     3421 gcgtgcgcca gcccgtgccg atgtacctga cggtgcggcc gctggaggtc ctgcgggcct
     3481 gaccggattg atatacactc tgtatatgaa acgtatatac ggagtcccgg catgggcatc
     3541 gtgaagatct cggacctgat gcacgacaac ctgcgcgacg ccagcgacgc gttgagccgg
     3601 tcgatcaact cgcaggccga gcactggctg cgcgtgggca tgctggccga gctgcacccc
     3661 gagctgaact acggcgacat ctgccagctg atgctgcgcg acgcgcgggc cggcggcggc
     3721 gggctgggct tcgtcaagcc ggaggtggcc gccgcggcct cggcgacggc cggcaaggcg
     3781 atccgcaggg cggcgcgatg aggcgcggcg cgaacctggt caagtccccc gaggagatcg
     3841 cgctggcgcg cgccgcgggc gcgctggcgg ccgacgtcct gcgcatgatc gcgccgcacg
     3901 tcaaggccgg cgtcaccacc gacgagctcg accgcctgtg ccacgaccac atcgtcggcg
     3961 tgcagaaggc ggtgccggcc aacgtcggct accacggcta cccgaagacg acctgcatct
     4021 cggtcaacca cgtcgtgtgc cacggcatcc ccgacggcaa ggcgctcaag gacggcgaca
     4081 tcctcaacat cgacgtcgcg gtcatcaagg acggctggta cggcgacacg agccgcatgt
     4141 actacgtcgg cacgccctcg gtgcacgcca ggcgcctggt ggacaccacc tacgaggcca
     4201 tgcgcgcggg catccgcagc gtcaggcccg gcgcgacgct gggcgacgtg ggccacgcga
     4261 tccagcaggt ggccgagaag gccggcttca gcatcgtgcg cgactactgc ggccacggca
     4321 tcggccggat ctaccacgac gacccgcagg tctgccactt cggccgccgc ggcgagggga
     4381 tgcggctgca ggagggcatg gtgttcacca tcgagcccat ggtcaacgcc ggcaagccgg
     4441 gcacgcgcga gctcgccgac ggctggacgg tggtcacgcg cgaccgctcg ctgtcggcgc
     4501 agtgggagca catggtggcc gtcacgcatg acgggttcga ggtattgaca ccgtggccgg
     4561 atggcaccgg tgcgtacgcc gcgatcggtt gagcgggtaa cagcgcgtgg ccgcctcggg
     4621 gaagtcccct aggagtctgc gcggcttctg ccgcgcagac tcctagggaa ccctgcccaa
     4681 cacgcgtaat tgctgacggg aaggccccgc ggccgttggc atactccgcg ctccgacaag
     4741 ccgcgtgagt cctgaccatg tcccgttcga acctggcgct gcgcatgatc tgcgccgccc
     4801 tggccggtgc cagcaccctg gcgcaggccg ccgcgaccac actcccggtc gcgcccgtga
     4861 aggccgtcac cgatacctac ttcggtacca ccgtcaccga cacctaccgc tacatggagg
     4921 acctgtcggc ccccgaagtg cagcagtggg ccaaggcgca ggccgaccac gcgcgcgagg
     4981 cgctcgacgc gatcccgggc cgcgcgccgc tgctggcgcg catccgcgag ctcgacgact
     5041 ccgtcaagga gcgcgtgcag tccgtcgcgc tggccggcgg cggcctcgtg ttctacgaga
     5101 agcgcggtgc cgacgacaac cagctcaagc tctacgtgcg ccagggctac aagggcgccg
     5161 agcgactgct ggtcgatcct gacgcccttg ccaaggccga cggcacgccg cacgccatcg
     5221 agttcttcca ggcgtccaac aacggcaggt acgtggccta cgcgatctcc gcgggcggct
     5281 cggaggaggc ggtcatccac gtgatggacg tggccaccgg caaggaggtg atggcgccga
     5341 tcgaccgcgc gcactacagc tacgtcgcat ggctgccgga tgactcgggc ttcttctaca
     5401 tgcgcctgca ggcgctcgcc aagggcgcgc ccgagacgga caagtaccag cgcgccaccg
     5461 catggttcca ccgcatgaag gggggcgcgg ccgacgtcgc cgtcctcacc gcgggcaccg
     5521 acacgcacct gaagatcgcc gcggccgaga tgccggtggt catgccggtc gtgggcacgc
     5581 gatggacggt ggcgatcccg gccaacggcg tcgagaacga gatcgacctg tacgccgcgc
     5641 cgcaaggcaa ggcgctcgac ccgaaggtca agtggcgcaa gctggtcggc cgcgaatcgc
     5701 aggtgaccgg cttcgcgatc cacggcgacg acctgtacct gctcacgcac ctgaacgcgt
     5761 cgcgctacaa ggtgatccgc acgagcatgt cgcatccaga cgtggcgatg gccgacgtcg
     5821 tcgtggcgcc gggcgaggag gtggtcgacc gcatcgccgc cgccaaggac gggctgtaca
     5881 tccagtcgcg cgacgccacc gtcggcaagc tgtaccgcct cgcatatgcg aaggacgcgc
     5941 agcccgagcc ggtgcagctg cccgcatccg gcgccgtgac catcgtcgac aacgacctca
     6001 cgcgtgccgg cgtcgtcgtc tccatcgact cgtggacgca cgacgaggcg ttctacagcg
     6061 tggccggcaa cggcggcccg gtggccgaca ccgggctgca ggtcgcgggc gcgttcggcg
     6121 cgccggccga cctcgtcgcc gaggaggtca aggtcaagag ctacgacggg ctcgagatcc
     6181 cgctgtccat cgtgcaccgc aaggacatga agctcgatgg cggcaatccg ctgatcctcg
     6241 aggcctacgg cgcgtacggc gtggtcgaca cgcccggcta cgcgccgcgc cagctggcct
     6301 ggtacgagct gggcggcgtg tacgccacct gccacgtgcg cgggggcggc atctacggcg
     6361 agaactggca cctggccggc aagcagctgt ccaagcccaa cagctggaag gacgcgatcg
     6421 cctgcggcga gtggctggtc aagaacggct acaccagctc ggccaggatg gcgctcaacg
     6481 gcggcagcgc cggcggcatc acggtcggtc gcgccatcac cgagcggccc gacctgttcg
     6541 cggtggcggt gcccgaggtg ggcgtgctca atgccgttcg ggccgagacc tcggccaacg
     6601 gcgtgcccaa catccccgag ttcggcagcg tcaaggacaa gcagcagttc aacggcctgc
     6661 tggagatgga cgcgttccac cacgtgcagg acggcgtgaa ctacccggcc acgctgctga
     6721 ccaccggcat caacgacccg cgcgtgccgg cctgggagtc gatgaagatg gccgcccgca
     6781 tgcaggccgc gacggcctcg ggcaagccgg tgctcctgcg catccagtac gacggcggcc
     6841 acggcatcgg ctcgaccaag aagcagcgcc aggaactggc ggccgaccgc tggtcgttca
     6901 tgctgtggca gttcggcgac gcgcggttcc agccggcgac caagtgacgt gaatcgatcc
     6961 cggcgccgcg gtgctcgcgc gcaggcgggc gcattcaggc gcgagcgggc gcgacgcgcg
     7021 cgcccccccg cgcgcgcgtg cgcgggcgat cgagcgccac gtcccaatac ggcgtctcgc
     7081 cgatcgcctc gcgcaggtgc gcgatgaaca gccgcgcctt ggcctccgac gagcgccccg
     7141 gcatgcgcac cgcatggatc gccggcagcg acgccgggtc ggcgggcttc atgtccggga
     7201 acagcgagac gagccggccg gcgacgatgt cctcgctgac gacccagctc gcaagaaagg
     7261 cgatcccgat gcccgccagc gcggcctgca gcgcgcagtc cttgtcgtcg atgcgcagct
     7321 tgccgcgcac gggcagcgcc gcgccgcggt tgacgccggc gaagcgccag gacggactgc
     7381 cggccgtggg cgcggggccc gtgatgcact cgtggcgcac gaggtcttcc ggccgcgcgg
     7441 ggcggccgtg ccgcgcgagg taggcggggc tggcgaccac cagcggacgc agcgtggcca
     7501 ggcgcgtggc gaccaggtcg gagtcgggca gcatgccgat gcggatggcc acgtccacgc
     7561 gctgctcggc gaggtcgacc agcgcgtcgc tcacgtgcag gtcgacctcg atcatcgggt
     7621 agcgctcgag aaagctcgcc acggcctgcg cgaggtggcg gcggccgaag atggtgggcg
     7681 cggtgacggt catggtgccg cgcgggtcgg cgtcgatcga tgcgagcgcg tccctggcct
     7741 gcgccagttc cgcgaggatg ttgcgggcgc gcggcaggaa ctgctcgccg gcatcggtga
     7801 gcatcacgcg gcgcgagctg cggtggaaca gccgcgtgcc gatctcggcc tccagccagt
     7861 cgatcttgcg cgtgaccgag gacacggcga cggcctgcgc cttggccacc tgcgagaagc
     7921 tgctggacga ggcgacggca aggaagctgt ggagggcgtc gatcatggat ctcgtggttg
     7981 cgtgaatcgc aacgatgctt tgcgaatatt gggattgttc tggaattcgc aaaacaacac
     8041 aatgcgatcc gcaccctcac cggccgaccc tccggccctc gacgaaagca accgatgacc
     8101 cgcttccaac gccttttcca actgagtgtc ctgtcagccg ccatcgccct ggggggcgcc
     8161 gcgctgctca ccgccgcgcc cgcggcccac gccgccgcgc cgcaggtgcg cacgcaggcg
     8221 cccggcttcc accgcgccat cctcggcgac ttcgagatca ccgcgctgag cgacggcacc
     8281 gtcgacctgg acgtgaccaa gctgctgcac gaacccgccg ccaggaccaa cgccgcgctc
     8341 gcgcgcgact tcgtcaagac accgctcgag acctcggtca acgcgttcct cgtcaacacc
     8401 ggcaccaagc tggtgctgat cgacaccggc gccggcacgc tgttcggccc cacgctgggc
     8461 aagctcgtcg acagcatcaa ggccgccggc tacacgcccg accaggtcga cgacatcttc
     8521 atcacgcact tccaccccga ccatgtcggc ggcctgtcgg ccaacggcga gcgcgtgttc
     8581 cccaacgcca cgctgcacgc ggacaaggcg gagtcggact tctggctgtc gcaggccaac
     8641 ctggacaagg cgccggccga catgaaggcc ttcttccagg gcgcgatggg ctcggtcaac
     8701 ccgtacgtcg cggccggcaa gtaccagccg ttcgaagccg acggcgagcc ggtgccgggc
     8761 atccgcacgc acgccacgca cggccatacg gcgggccaca cgagctacgt cgtcgagagc
     8821 aagggccagc gcctggtcgt gatcggcgac ctgatccacg tggccgcggt gcagttgccc
     8881 aacccgggcg tgacgatcgc gttcgactcc gacgccaagg ccgccgcggc ggcgcgagat
     8941 gcggtcttca ggcaggcggc caaggacggc tcgctggtgg ccgccgccca cctgcagttc
     9001 cccggcctgg gccacttgcg cgcggccggc aagtcgtggc agtgggtgcc ggtgaattac
     9061 acgaccgagc tcaagtgagt gtccggcggc ggcacgcgcg tcagaactgc ttgaacaggt
     9121 gcgtgtagga cgcgctgacg tccaggcgct cgggccgctg cttcaggcgc agcagcatcc
     9181 ggccggtcgg cgtgcgtgcg acgctgtgca ctgcgcgcat gttgaccacg gtgctgcggt
     9241 gcacctgcca gaacagggcc ggatcgagcc gcggcagcag gtccttcagc ggcgtggtga
     9301 tcaaggcctc gccgtcggtg gtgaccactg ccacgtactt gtggtccgcg cggaagtagc
     9361 agacgtcgtc gacctcgatc agcttgacgt cccggccctg caggacgctg atccagcgca
     9421 gcggcgacga cggcgcgccc gcgtcgctcg ccgcaggcgc cgggacgggc gctgccggtg
     9481 gcgcggcatc ggccaggcgc tccttcaggc gctgcacggc gcgggcgaga cgctccgtct
     9541 ccaccggctt gagcaggtag tcgaccgcgc cttcgtcgaa ggccgacacg gcgtagcggt
     9601 cgaaggcggt gacgaagaca acgtgcggcc gcgggccggc aagacgcgcc agggcgacgc
     9661 cgtccaggcc gggcatctgg acgtccagga acagcacgtc gggcttgcgg gcctgcacct
     9721 ggcgcaaggc gtcgtggcca tcgtgcacga ccgcgcagat ctcgagctcg ggccagagct
     9781 gcgcgagctc gtcgcggagt tcggcggcca gcaacggctc gtcctcagcg atcagggccc
     9841 tggcggtcat cgggcacggg caggtggaga tggaggccgc aggcggcgtc gcgctcggcg
     9901 atcgccaggc gggcgcccgg gccgtgcacg ccgcgcaggc gcgcgagcag cgacggctgg
     9961 tcgacgccgg ccagggcatg ccgggcggcg gcatcggccg gctcggccac gacggcgatg
    10021 gccagcgcgt ccgcttcgag gtgagcgtcc aggcgccagc gggacggccc gtgcctggcc
    10081 aggtcgcgca gcagcggcag cagaacgccc ggcgccaggg cggtggcgcg cgcctgcgcg
    10141 ctcgcggaca tcaacagccg cggcggacgc aaccccgagc gcgcgctcgt gcgggcatag
    10201 gcgctcacga gcgagagttc gcgctgaagg gacgaggccg cctgcgcgcc gccgccctgg
    10261 agtgcgcgga gcaggtgcgt cagcgccatc agcagtcggt ccgcagcggc agtggagcgg
    10321 cgggcgaggc gattgacgcg gcccagcgtg tcgaacagga actcgggctc gacgcgggcc
    10381 tgcagcgcga gcagccgggc cgtctcgacg gcgcgttggc gcgccgcctg ctcgatctct
    10441 ccgcggcgca gcgccgcgag cgcgcgctgc tcgccgcgcc aggtcgcgtg gacgaacgtc
    10501 agcgtgccga gcgacagcat gcgtcccaac gcgagggaca ggtcgtccat gacgttccac
    10561 gtccccggga tgccaccgag cgcgagcacg atgaggttgc ccaggagcca gatg
//
LOCUS       DATCRO010000574         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_681522, whole genome shotgun sequence.
ACCESSION   DATCRO010000574 DATCRO010000000
VERSION     DATCRO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681522"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VIN75_15775"
     CDS             <1..1075
                     /locus_tag="VIN75_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975678.1"
                     /translation="LKGVSVDIPDRAFAVLVGPSGCGKSTLLRLLAGLEQADGGTIRF
                     GDRDDVTDHQPARRGIAMVFQSYALYPHMNLYDNMAFGLKIAKVDKQEIHRLVHQAAK
                     ILNIEHLLDRKPKALSGGQRQRVAIGRAIVRKPEVFLFDEPLSNLDAALRVRMRYEFA
                     KLHEDLKTTMIYVTHDQVEAMTLADRIVVLAAGRIEQVGSPLALYEHPDNVFVAGFIG
                     SPKMNFLEATVVSGDASGAVVKLQTGAQIRCEVDAARAAAGEKVTLGVRPEHFEAAAP
                     GATDNVIATSVTFVESLGSTTHAYCEYPGAAETLVCELDGATRLRNGAPVALAIPAAR
                     TYLFDAAGQAFRRHVAQTQQEAA"
     gene            1072..>2215
                     /gene="malE"
                     /locus_tag="VIN75_15780"
     CDS             1072..>2215
                     /gene="malE"
                     /locus_tag="VIN75_15780"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HEY8975679.1"
                     /translation="MTTRPALRRVAARIVAGALACSSVVQAAEPLKLLVWINPDKGYN
                     GLQKVGDRYRADTGVEVTVQHPDDAPSKFQAAAGAGKGPDIFCWPHDRAGEWAKSGLI
                     VPIKPRRAVHDAIEESAWQAFNYQGRTWGYPLSIEAIGLIWNKAMLKEPPRTWDDVVA
                     IDKELSAHGKKAILWDYNKSFFSWPLLAGSGGTIFGRKPDGSFDTDVVGVNAPGAVQA
                     MTMLQNLVRTGVLPKGAGYSEMEGAFNRGEIAMMISGPWAWDNVRKSHIDFGVAPIPS
                     VNGHEPKPFVGVLGCMIAAPSRNKDLARDFLEHYVLTLDGLRTINADVPLGTPANKAF
                     YAELKADPHIAATMDNARRGAPVPNIPEVGKFWTAMDAAFEAVTNMR"
BASE COUNT          372 a          805 c          741 g          297 t
ORIGIN      
        1 cctcaagggc gtgagcgtcg acatccccga ccgcgccttc gccgtgctgg tcgggccgag
       61 cggctgcggg aagtcgacgc tgctccgcct cctcgccggg ctggagcagg cggacggggg
      121 caccatccgc ttcggcgacc gcgacgacgt caccgaccac cagccggccc ggcgcggcat
      181 cgcgatggtg ttccagtcgt acgcgctgta cccgcacatg aacctgtacg acaacatggc
      241 gttcggcctc aagatcgcca aggtcgacaa gcaagagatc catcgcctgg tgcaccaggc
      301 cgccaagatc ctcaacatcg agcacctgct cgatcgcaag cccaaggcgc tgtcgggcgg
      361 ccagcgccag cgcgtggcca tcggtcgcgc catcgtgcgc aagcccgagg tcttcctgtt
      421 cgacgagccg ctgtccaacc tcgacgcggc cctgcgcgtg cggatgcgct acgagttcgc
      481 caagctgcac gaggacctga agaccaccat gatctacgtc acccacgacc aggtcgaggc
      541 catgacgctg gccgaccgca tcgtggtgct ggccgccggc cgcatcgagc aggtcggctc
      601 gccgctcgca ctgtacgagc atccggacaa cgtgttcgtg gccggcttca tcggctcgcc
      661 caagatgaac ttcctggagg ccaccgtggt cagcggcgac gcgagcggtg ccgtcgtcaa
      721 gctgcagacg ggggcgcaga tccgctgcga ggtcgatgcc gcgcgcgcgg ccgccggcga
      781 gaaggtgacc ctgggcgtgc ggcccgagca cttcgaggcc gcggctcccg gtgcgacgga
      841 caacgtgatc gccaccagcg tcaccttcgt cgagtcgctg ggctcgacca cgcacgccta
      901 ctgcgagtac cccggcgccg ccgagacgct ggtgtgcgag ctggacggcg ccacgcgcct
      961 gcgcaacggc gcgccggtcg cgctggcgat ccccgctgcg cgcacctacc tgttcgacgc
     1021 cgcgggccag gccttccgcc ggcacgtcgc ccagacgcag caagaggccg catgacgacg
     1081 cgccccgccc tgcgccgcgt cgcggcgcgc atcgtcgcgg gcgcgctcgc atgctcgtcc
     1141 gtcgtgcagg ccgccgagcc gctcaagctg ctggtctgga tcaaccccga caagggctac
     1201 aacggcctgc agaaggtcgg cgacaggtac cgcgccgaca cgggcgtcga agtcaccgtg
     1261 cagcaccccg acgacgcccc gtccaagttc caggccgccg ccggcgccgg caagggcccc
     1321 gacatcttct gctggccgca cgaccgcgcc ggcgagtggg ccaagtcggg gctgatcgtg
     1381 ccgatcaagc cgcgccgggc ggtgcacgac gccatcgagg aatcggcctg gcaggccttc
     1441 aactaccagg gccgcacctg gggctacccg ctgtcgatcg aggccatcgg cctgatctgg
     1501 aacaaggcga tgctcaagga gccgcccagg acgtgggacg acgtcgtcgc catcgacaag
     1561 gaactgtcgg cgcacggcaa gaaggccatc ctctgggact acaacaagag cttcttcagc
     1621 tggccgctgc tggccggctc gggcggcacg atcttcggca ggaagcccga cggcagcttc
     1681 gacaccgacg tcgtgggcgt caatgcgccg ggcgccgtgc aggccatgac gatgctgcag
     1741 aacctcgtca ggaccggcgt gctgcccaag ggcgcgggct actccgagat ggaaggcgcg
     1801 ttcaaccgcg gcgagatcgc gatgatgatc tcgggcccct gggcctggga caacgtcagg
     1861 aagagccaca tcgacttcgg cgtcgcgccc atcccctcgg tcaacggcca tgagcccaag
     1921 cccttcgtgg gcgtgctggg ctgcatgatc gccgcgccca gcaggaacaa ggacctggcg
     1981 cgcgacttcc tcgagcacta cgtgctgacg ctggacggcc tgcgcacgat caacgccgac
     2041 gtgccgctgg gcacgcccgc gaacaaggcc ttctacgccg agctcaaggc cgacccgcac
     2101 atcgccgcga cgatggacaa cgcgcgccgc ggcgcgccgg tgcccaacat ccccgaggtg
     2161 ggcaagttct ggacggccat ggacgctgcg ttcgaagccg tgaccaacat gcgcc
//
LOCUS       DATCRO010000575         8029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_749181, whole genome shotgun sequence.
ACCESSION   DATCRO010000575 DATCRO010000000
VERSION     DATCRO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749181"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1554
                     /gene="kynU"
                     /locus_tag="VIN75_15785"
     CDS             238..1554
                     /gene="kynU"
                     /locus_tag="VIN75_15785"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY8975680.1"
                     /translation="MITRDDCARRDAEDALAPLRDQFMLPEGVIYVDGNSLGALPRAT
                     PARVAAAVQQEWGDGLIRSWNDAGWITLPQRVGDRIGALIGAAPGECVAADSTSVNLY
                     KVLSAAARIAQADAPARRVIVSERQNFPTDLYIAEGLCRALGWTLELLEPPAIVERLS
                     NGRGADVALLMLTQVNYRTGALHDMAAVTALAHAAGALTIWDLAHSAGALPVDLNDAG
                     ADFAIGCGYKFLNGGPGAPAFVWAHPRHAERFWQPLSGWMGHAAPFMFRPNYEPAPGI
                     GRFLCGTPAVLSLTALECGVDTLHAADALGGMAALRAKSIALTEAFIALVDERCAPLG
                     FAVASPRDARLRGSQVSLTHASPDGASHGYAIVQALIARGVIGDFRAGTPPDACGPAL
                     PDILRFGFTPLYLRFVDVYDAVDHLVQVMRTGEFREARFNKRSAVT"
     gene            1608..2489
                     /gene="kynA"
                     /locus_tag="VIN75_15790"
     CDS             1608..2489
                     /gene="kynA"
                     /locus_tag="VIN75_15790"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEY8975681.1"
                     /translation="MGHGADPAPAAAEVDARGERIVAEEGAQLDFESDMSYGDYLHLD
                     QVLTAQHPLSPDHNEMLFIVQHQTSELWMKLMLHELRAAVASIAADELGAAFKMMARV
                     SRIMEQLVHAWDVLATMTPSEYSAIRPYLAASSGFQSWQYRCIEFAMGNKNAAMLKPH
                     SHRPDLLAQVELASRAPSLYDESLRLLARRGLPIPADYLERDWTQPYVESAAVRDAWL
                     AVYRDPNRHWDLYQLGEELTDLEDAFRLWRFRHLTTVQRIIGFKRGTGGTGGVSYLRK
                     MLDTVLFPEVWALRTEL"
     gene            2822..3460
                     /locus_tag="VIN75_15795"
     CDS             2822..3460
                     /locus_tag="VIN75_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975682.1"
                     /translation="MSALTAADASDDARYVEHWIHAKGDDHGHPFVIVDKKAARIFVF
                     GAGGKLVGESSTILGQSRGDVPVPGAGQKNPSDLLPFERKTPAGRFESQPGRNLHGEP
                     VVWVDYDTGIAIHRVRPGISQMQRVNSLATDDPNNKRLSLGCVVVPEDFFWSVVIPTM
                     GHSHGVVYVLPEDEPVQALFVAPKSEPKAVAATASIAPRAVAQPVRLDVSAR"
     gene            complement(3465..4379)
                     /locus_tag="VIN75_15800"
     CDS             complement(3465..4379)
                     /locus_tag="VIN75_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8975683.1"
                     /translation="MARIPLQFLPTFVAVARLQTLRAAADSLHLTHSAVSQQIGELES
                     RLGFPLFDRRGRRVHLNAAGEALLRHASPALAQVDDGVQAAAAAAQGAAQGIRITMLP
                     SFAQRWFLPRLGAWRARHPDIPIDIEASAGIIDLQRDGFHAAIRTGAGPWPGLVSERL
                     YESPTPFIAVAAPAVARRLREGQPADLAREPLLGDSEVWEKWFAGAGVHARATPVASF
                     NDVGLMLQATEQGLGLSVVRELLAADALRDGRLARLFDVSVVLDGVQPYSFAFPPALK
                     EWAPLVALRAWVREEFDASLATLKTIAR"
     gene            4474..4728
                     /locus_tag="VIN75_15805"
     CDS             4474..4728
                     /locus_tag="VIN75_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975684.1"
                     /translation="MTTFTITPTVTPSLASRLLEAASRYFSRSVVTRESVDQAWIDSG
                     LQRLDAATLKDIGAPAGVIEGASVREAWKLAAALDATRHL"
     gene            4884..5075
                     /locus_tag="VIN75_15810"
     CDS             4884..5075
                     /locus_tag="VIN75_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975685.1"
                     /translation="MTKSTAQAAAFALSILMTLGIVAGMNGIATKQYAAAERVAQVAA
                     DATQVAALQRVVVVARRAA"
     gene            complement(5153..6100)
                     /locus_tag="VIN75_15815"
     CDS             complement(5153..6100)
                     /locus_tag="VIN75_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975686.1"
                     /translation="MPVTFRSALLACTIAAGAALTGCDSLGPTLKDSAYSLERSRAGL
                     ERKEMTLADGTRMVWLEGGAGAPLVLVHGFGADKDNFTRVARFLTPHYRVIAPDLVGF
                     GESAHRADVDYHYAAQAERLHAFVQALGLSRIDLGGNSMGGGIAMSYAAQHPTEVASL
                     WLIDCAGIAGAPPSELAKIVTTTGQNPLMIARESDFTAFLDFVMSDPPYIPGPVKNVM
                     ARERIANQALERQVFQQIATDSVSASVKGLATPTLITWGDEDRALSVGTVPILETLLP
                     NAQAVVMPHVGHAPMIERPKESAEDYLRFREGLSRHAAQ"
     gene            complement(6529..7989)
                     /locus_tag="VIN75_15820"
     CDS             complement(6529..7989)
                     /locus_tag="VIN75_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8975687.1"
                     /translation="MDAPLDLRHAADDAGPARARHAQAPAPRAPAPAVSANGPRLGEL
                     LGALSHALDMTEGQPVGHCMRSCWIGQQLGRAIGLSTAELRELYYTTLLKDSGCSSNA
                     ARLCELFLTDDLVFKRARRTVGMSLPSLLRFVVRHTGPAAGMRERVRAIANAVQNGGE
                     IHRELTEQRCSRGAQIARQLRFSDDVAEAIQSLDEHWDGSGHPQGLAGKEIPLLARLA
                     LLAQAVDVFHTSAGPQATREEIARRSGRMFDPRLADAFAVLARDPAFWRQLACDDLEQ
                     AVLEMEPAQQPQPVDDDYLDDIAAAFGQIVDAKSPWTGGHSARVGLITEQLARELGLA
                     APERRWLRRGALLHDIGKLGVSNRILDKAGPLDIAEWIVVQRHAMHTEQILGRISSFG
                     VLARVAGAHHEKLDGTGYPRGLDARHIRLETRIITTADVFDALTADRPYRAAMPTGRA
                     LQVMRDSIGTAIDGDCFAALERSLDALAQFSAFGGL"
BASE COUNT         1149 a         2887 c         2836 g         1157 t
ORIGIN      
        1 cgctcgacca cggtgatgtc gtgccccggg tcaagcttct tcatcagcag ggcaaaggtg
       61 aggccggcgg ggccgccgcc gatgcagacg atgcgcattg aagtcctcat ctatcctgga
      121 gagcgctggc gccggcgact gcccggcgcc aattgcttga ggtatgaata gtttaatact
      181 aaactaattg catcccattc cgcaagatgg ccttcggggc cgtcaggagc gaccgccatg
      241 atcaccagag acgactgcgc tcgacgcgac gccgaggacg cgctcgcccc gctgcgcgac
      301 cagttcatgc tgccagaagg cgtgatctat gtcgacggca actccctggg cgcgctgccc
      361 cgcgccacgc ccgcgcgcgt cgctgccgcg gtgcagcagg aatggggcga cggcctgatc
      421 cgcagctgga acgatgccgg ctggatcacg ctgccccagc gcgtgggcga ccgcatcggt
      481 gcgctgatcg gcgccgcgcc gggcgagtgc gtcgcggcgg actcgacctc ggtcaacctg
      541 tacaaggtgc tcagcgccgc cgcgcgcatc gcgcaggcgg acgcgcccgc gcgccgggtc
      601 atcgtcagcg agcgccagaa cttccccacc gacctgtaca tcgccgaggg cctgtgccgc
      661 gcgctgggct ggacgctgga gctgctcgag ccgccggcga tcgtggaacg cctgtcgaac
      721 ggacgcggcg ccgacgtcgc gctgctgatg ctcacgcagg tcaactaccg caccggcgcg
      781 ctgcacgaca tggccgcggt gaccgcgctc gcgcacgcgg ccggcgcgct gacgatctgg
      841 gacctggcgc actccgccgg cgcactgccg gtcgacctga acgacgccgg cgccgacttc
      901 gcgatcggct gcggctacaa gttcctcaac ggcggcccgg gcgcgccggc cttcgtgtgg
      961 gcgcacccgc gccacgccga gcgcttctgg cagccgctgt ccggctggat gggccacgcc
     1021 gcgccgttca tgttcaggcc gaactacgag cctgcgcccg gcatcggacg cttcctgtgc
     1081 ggcacgcccg cggtgctgtc gctgaccgcg ctggaatgcg gcgtcgacac gctgcacgcc
     1141 gccgacgcgc tgggcggcat ggcggcgctg cgggcgaagt cgatcgcatt gaccgaggcc
     1201 ttcatcgcgc tggtcgacga gcgctgcgcg ccgctgggct tcgcggtcgc gagcccgcgt
     1261 gacgcgcgcc tgcgcggcag ccaggtcagc ctgacgcacg cgagtcccga cggcgcctcg
     1321 cacggctacg ccatcgtgca ggcgctgatc gcgcgcggcg tgatcggtga cttccgcgcc
     1381 ggcaccccgc ccgatgcctg cggccccgcg ctgcccgaca tcctgcgctt cggcttcacg
     1441 ccgttgtacc tgcgcttcgt cgacgtgtac gacgcggtgg atcacctggt gcaggtgatg
     1501 cggacgggcg aattccgcga agcgcgcttc aacaagcgct ccgcggtgac ctgagcgggg
     1561 aaaatcacgc catgagccag accaacgaca ccgccggccg ctgcccgatg ggccacggcg
     1621 ccgaccctgc acccgccgcc gccgaggtcg atgcgcgcgg cgagcgcatc gtcgccgagg
     1681 agggcgccca gctcgatttc gagtcggaca tgagctacgg cgactacctg cacctggacc
     1741 aggtgctgac cgcccagcac ccgctctcgc cggaccacaa cgagatgctg ttcatcgtgc
     1801 agcaccagac gtccgagctg tggatgaagc tgatgctgca cgagctgcgc gcggcggtcg
     1861 cctccatcgc cgccgacgag ctcggcgccg cgttcaagat gatggcgcgc gtcagccgca
     1921 tcatggagca gctggtgcat gcgtgggacg tgctggcgac gatgacgccg tccgagtatt
     1981 cggcgatccg tccctacctg gcggccagca gcggcttcca gagctggcaa taccgctgca
     2041 tcgagttcgc gatgggcaac aagaacgccg cgatgctcaa gccgcattcg caccgccccg
     2101 acctgctcgc gcaggtggag ctggcctcgc gcgcgccgtc gctgtacgac gagagcctgc
     2161 gcctgctcgc gcgccgcggc ctgcccatcc ccgccgacta tctcgagcgc gactggacgc
     2221 agccctacgt ggagagcgcc gccgtgcgcg acgcctggct ggcggtctac cgcgatccga
     2281 accgccactg ggacctgtac cagctgggcg aggagctgac cgacctggag gacgcgttcc
     2341 gcctctggcg cttccgccac ctgacgacgg tgcaacgcat catcggcttc aagcgcggca
     2401 ccggcggcac cggcggcgtc agctacctgc gcaagatgct cgacacggtg ctgttccccg
     2461 aggtctgggc gctgcgcacc gaactgtgac agcgcgtcgc catgcgggtt tcctgctcga
     2521 caggatgcgg cgacttggtg caaattgcca accgatgagc gagtgttccg tcgagtagaa
     2581 gatcgtaatt ggcgtgcttt attgccgcaa acccttcata ttcaccgtaa gatctgcccc
     2641 taccatgtag gacaagggcg cgaaatcgtg cccgtccccg gcacttccaa ccggggccac
     2701 gagcaggatc ggaatcgtcg agatgaagtt ttggggcaag ctggcagtcg ccgcaacggt
     2761 cgcgtgggcc tcgtccacgg ctttcgcact caccgacccg acgtctcgcc ctggcgagcc
     2821 cttgtccgcg ctgaccgccg cggacgcgtc ggacgatgcg cgctacgtgg agcactggat
     2881 ccacgccaag ggagacgacc acggccaccc tttcgtgatc gtcgacaaga aggcggcgcg
     2941 catcttcgtg ttcggcgccg gcggcaagct cgtgggcgag tcgtccacca tcctcggcca
     3001 gagccgcggc gacgtgcccg tgccgggcgc gggccagaag aatccgtccg acctgctgcc
     3061 cttcgagcgc aagacgcccg caggccgctt cgaatcgcag ccgggccgca acctgcacgg
     3121 cgagccggtg gtctgggtcg actacgacac cgggatcgcg atccaccgcg tgcgcccggg
     3181 catctcgcag atgcagcgcg tcaacagcct ggccaccgac gacccgaaca acaagcggct
     3241 ctcgctcggc tgcgtcgtgg tgcccgagga cttcttctgg tcggtcgtca tcccgacgat
     3301 gggccacagc cacggcgtgg tctacgtgct gcccgaggac gagccggtgc aggcgctgtt
     3361 cgtggcgccc aagtccgagc ccaaggccgt cgccgcgacc gcgtcgatcg cgccgagagc
     3421 ggtcgcccag cccgtccgcc tggacgtcag cgcccgctga gccttcagcg cgcgatggtc
     3481 ttgagcgtcg ccagtgacgc gtcgaactcc tcgcgcaccc acgcccgcag cgccacgagc
     3541 ggcgcccact ccttgagcgc cggcgggaac gcgaagctgt agggctggac gccgtccagc
     3601 accaccgaca cgtcgaacag ccgcgccaga cggccgtcgc gcagcgcgtc ggccgcgagc
     3661 agttcgcgca ccactgacag gcccagtccc tgctcggtgg cctgcagcat caggccgacg
     3721 tcgttgaacg atgccaccgg cgtcgcgcgc gcgtgcacgc cagcgccggc gaaccacttc
     3781 tcccacacct cgctgtcgcc cagaagcggc tcgcgcgcga ggtcggccgg ctggccctcg
     3841 cgcaggcggc gcgccaccgc gggcgcggcc acggcgatga acggcgtggg cgattcgtac
     3901 agccgctccg acaccagccc cggccagggt cccgcgcccg tgcggatcgc ggcatggaag
     3961 ccgtcgcgct gcaggtcgat gatgccggcg gaggcctcga tgtcgatcgg gatgtcggga
     4021 tggcgcgcgc gccaggcgcc cagccgcggc aggaaccagc gctgcgcgaa ggacggcagc
     4081 atcgtgatgc ggatgccttg cgcggcgccc tgggccgccg cagccgcggc ctgcacgccg
     4141 tcgtccacct gcgccagcgc gggactcgca tgccgcagca gcgcctcgcc ggccgcgttc
     4201 aggtgcacgc ggcgcccgcg ccggtcgaac agcgggaagc cgagccggct ttcgagctcg
     4261 ccgatctgct ggctgaccgc gctgtgcgtc aggtgcaggg aatcggccgc cgcgcgcagc
     4321 gtctgcagcc gggcgaccgc gacgaaggtg ggcaggaact ggagtgggat gcgggccatt
     4381 ggtcagtcca gcttaccaat ttctgcagaa caagtcgata tacgcgggcc aatgtccgtc
     4441 ctattcttac caacacaaga acaggagatc accatgacca cgttcaccat caccccgacc
     4501 gtcaccccgt cgctcgcgag ccggctgctg gaagcggcgt cccgctactt cagccgctcc
     4561 gtggtcacgc gcgagagcgt cgaccaggcc tggatcgact cgggcttgca gcgtctcgac
     4621 gcggccacgc tcaaggacat cggcgcgccg gccggcgtca tcgaaggcgc ttccgtccgc
     4681 gaggcctgga agctggcggc ggcgctggac gcgacgcgcc acctctgagt cgccggacgc
     4741 cggtccgtcg gccgcggcga cgtctggcgc cgtcgacggg ccggctcgtc gcaagccggt
     4801 ttgaacgccg gacgccatcc cggacagtcc acccatcgga acacgaaacg cgatcccgaa
     4861 ccaaccaccc caaggagccc atcatgacca agtccaccgc ccaagccgcc gccttcgcgc
     4921 tgtccatcct catgaccctc ggtatcgtcg ccggcatgaa cggcatcgcc accaagcagt
     4981 acgcggccgc cgagcgcgtg gcccaggtcg cggccgacgc gacgcaggtc gccgcgctgc
     5041 aacgcgtggt cgtggtggcg cgccgcgccg cctgatccgc ctgcgccgct ccgccgagcc
     5101 ccgcttcggc ggggcttttt catggcggct cggcgctgcg caggcgcaga cctcattgcg
     5161 ccgcgtgcct ggacaggccc tcgcggaagc gcaggtagtc ctcggccgat tccttcggcc
     5221 gctcgatcat cggcgcatgg ccgacgtgcg gcatcacgac cgcctgcgcg ttgggcagca
     5281 gcgtctcgag gatcgggacg gtgcccaccg acagcgcgcg gtcctcgtcg ccccaggtga
     5341 tcagggtggg cgtcgccagc cccttgaccg acgcgctcac cgaatccgtg gcgatctgct
     5401 ggaacacctg cctttccagc gcctggttgg cgatgcgctc gcgcgccatc acgttcttga
     5461 ccggccccgg gatgtacggc gggtcgctca tcacgaagtc caggaatgcg gtgaagtcgc
     5521 tttccctggc gatcatcagc gggttctggc cggtggtcgt cacgatcttg gcgagctcgc
     5581 tgggtggcgc cccggcgatg ccggcgcagt cgatgagcca caggctggcc acttccgtcg
     5641 ggtgctgcgc ggcatagctc atcgcgatgc cgccgcccat cgagttgccg cccaggtcga
     5701 tgcgcgacag tcccagcgcc tggacgaagg cgtgcaggcg ctcggcctgt gcggcgtagt
     5761 ggtagtcgac gtcggcccgg tgcgccgatt cgccgaagcc caccaggtcg ggcgcgatca
     5821 cgcggtagtg cggcgtgagg aagcgcgcga cgcgggtgaa gttgtccttg tcggccccga
     5881 agccgtgcac cagcaccagc ggcgcgccag cgccgccctc cagccagacc atgcgcgtgc
     5941 cgtcggccag cgtcatctcc ttgcgctcca ggccggcccg cgagcgctcc agcgaatagg
     6001 cgctgtcctt gagcgtcggc cccaggctgt cgcagccggt gagcgcggcg ccggcggcga
     6061 tcgtgcaggc gagcagggcg gagcggaacg tgacgggcat ggcgggcagg cgttgaggcg
     6121 acgacgcgac tgtagcggcg gcgccgcggt cgcgcgattc gctttcgggg cggcgcggct
     6181 gacccgcgtg agagccccgt cgtgcgcgtg tcggcggcgg cgcaactcgc atttccagcg
     6241 tctcgcgcgg gaccggcgtg gccgctgcgc gcctacccgg tgtcatcctg ctcgcagcga
     6301 agcgaagtcg caggatccac ctcggcgatg cgatttggta gccaccgtgg atcctgcgac
     6361 tgcgcgcagg atgacacggg gtgggcgcgc cgcgagggcc gggtcggcgt gccgcgacgg
     6421 tcgggtcggc gctccgccat ggtcaggtct gcgctccgcc gtggccggat cggtgccgcc
     6481 gacggggccc ggccggggcc tcgcggccac ggggcgttcg tccgcccctc agaggccgcc
     6541 gaacgcgctg aactgcgcga gcgcgtccag cgaacgctcc agcgcggcga agcagtcgcc
     6601 gtcgatcgcc gtgccgatgc tgtcgcgcat cacctgcagc gcccggccgg tgggcatcgc
     6661 cgcgcggtag gggcggtcgg cggtgagggc gtcgaagacg tcggccgtcg tgatgatgcg
     6721 cgtctccagc cggatgtggc gggcgtccag gccgcgcgga tagccggtgc cgtccagctt
     6781 ctcgtggtgc gcgccagcca cgcgcgcgag cacgccgaac gaactgatgc gcccgaggat
     6841 ctgctcggtg tgcatcgcgt ggcgctgcac gacgatccat tcggcgatgt ccagcgggcc
     6901 ggccttgtcg aggatgcggt tgctcacgcc cagcttgccg atgtcgtgca gcagcgcgcc
     6961 gcgccgcagc cagcgccgct cgggcgcagc caggcccagc tcgcgcgcca gttgctcggt
     7021 gatgagcccg acgcgggcgc tgtggccgcc ggtccacggg ctcttggcat cgacgatctg
     7081 gccgaaggcg gcggcgatgt cgtccaggta gtcgtcgtcg acgggctgcg gctgctgcgc
     7141 cggctccatc tccagcaccg cctgctcgag gtcgtcgcag gcgagctggc gccagaacgc
     7201 ggggtcgcgc gcgagcacgg cgaaggcgtc ggccaggcgc gggtcgaaca tgcggccgct
     7261 gcggcgcgcg atctcctcgc gcgtggcctg cgggccggcg ctggtgtgga acacgtcgac
     7321 ggcctgcgcc agcagcgcca ggcgcgccag cagcgggatc tccttgcccg ccaggccctg
     7381 cggatggccg ctgccgtccc agtgctcgtc gagcgactgg atggcctcgg ccacgtcgtc
     7441 ggagaagcgc agctggcggg cgatctgcgc gccgcgcgag cagcgctgct cggtcagctc
     7501 gcgatggatc tcgccgccgt tctgcaccgc gttggcgatc gcgcgaacgc gctcgcgcat
     7561 gccggcggcc gggccggtgt ggcgcacgac gaagcgcagc aggctgggca ggctcatgcc
     7621 cacggtgcgg cgcgcgcgct tgaagacgag atcgtcggtc aggaacagct cgcacaggcg
     7681 cgcggcgttg ctgctgcagc ccgagtcctt gagcagcgtc gtgtagtaga gctcgcgcag
     7741 ttcggccgtc gacaggccga tggcgcggcc gagctgctgg ccgatccagc aactgcgcat
     7801 gcaatggccg acgggttggc cctcggtcat gtccagcgcg tggctgagcg cgcccagcag
     7861 ctcgcccagc cgcgggccgt tggccgacac cgcgggcgcc ggcgcgcgcg gcgcgggtgc
     7921 ctgcgcgtga cgcgcgcggg cgggaccggc gtcgtcggcg gcgtggcgga gatcgagcgg
     7981 ggcatccatc cagggtctat cggcccggca gtggggcggc tgaagtccc
//
LOCUS       DATCRO010000576         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_96272, whole genome shotgun sequence.
ACCESSION   DATCRO010000576 DATCRO010000000
VERSION     DATCRO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96272"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1513)
                     /locus_tag="VIN75_15825"
     CDS             complement(245..1513)
                     /locus_tag="VIN75_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8975688.1"
                     /translation="MTRPESRASTLQRLAVLTAAVAAGTALAPQAALAANPQCQSWVS
                     SIAYVGGDTAVRNGVSYKANWWTQGQDPATNNGGAGSGQPWTQITSCGTTPPPPTPAP
                     PPPTPAPPPPTPAPPPPTPAPPPPTPTPPPSPTPAPPPSGHWLVGYWHNFTNPSGATF
                     PISQVSNNWDTIIVSFADNAGNGNISFTVDPNAGSDAQFIADVAAKRKAGKKVVLSVG
                     GQNGSISLDNATMVTNFVNSAYAIIQKYGFDGIDLDLETGVSQNTALQTNLVTAMRQL
                     KAKVGSNFYLSMAPEHPYVQGGYVAYGSIWGAYLPIIDQLRNDLTEIHVQYYNNGGFT
                     YDNASNQQVSEGTVDGLVAGSLMLIEGFSVSYGTGWHFNGLRPDQVAFGVPSGPSSAN
                     TGFVTSTVVANTLNCLTKLQGCNTIKPAKA"
     gene            complement(1624..>2581)
                     /locus_tag="VIN75_15830"
     CDS             complement(1624..>2581)
                     /locus_tag="VIN75_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8975689.1"
                     /translation="PTPTPPSPPPSGGVMYGPYKDITVSMNWNTNVISTAVTGTLSPV
                     LSVKPAKLKMITWAFATGACGSENWGGLQASAVASANVQNFVNAGVRYIISTGGAAGS
                     FTCASDTDFETFVNRYASANLAGIDFDIEAGQTSADIDNLVARVKASQSKHPGLRWSF
                     TLATLGGNSPQSLGSMGVTVMNSIKSHGLTGYIINLMAMDYGSAIASNCTLNSSGKCD
                     MGKSAIQSAVNLHNYWGVPYSNIEITPMIGGNDATDETFSIADVTTLSNWVKANGASG
                     IHFWSLDRDKDCAPGYASATCNSYGTAGTWGFTNAFISALGL"
BASE COUNT          345 a          795 c          987 g          454 t
ORIGIN      
        1 cgctcatcga ggaggccgcg ccgaagtgga gcgccgcggc gtggcagtcg cgcggcacga
       61 ggtagcggcc gcgcgagcgg cgcggccatg aagaaggggc gcccgcgggc gccccttcgt
      121 tcctgacgat cgatggatcg gatcagggca gcgcgttcag cgccgcgcgc gtcggcacgg
      181 agaagttgta gccgtcgtgc ttgtcccagt tgatggacca cgtcatcgcg ccgcggaagg
      241 tcggttaggc cttcgccggc ttgatggtgt tgcagccctg cagcttcgtc aggcagttca
      301 gcgtgttggc caccaccgtc gacgtcacga agccggtgtt ggccgagctg gggcccgagg
      361 gcacgccgaa ggccacctgg tcgggacgca ggccgttgaa gtgccagccc gtgccgtacg
      421 acacgctgaa gccctcgatc agcatcaggc tgcccgcgac gaggccgtcg acggtgcctt
      481 ccgacacctg ctggttgctg gcgttgtcgt aggtgaagcc gccgttgttg tagtactgca
      541 cgtggatctc ggtgagatcg ttgcgcagct ggtcgatgat gggcaggtac gcgccccaga
      601 tcgagccgta ggcgacgtag ccgccctgca cgtacgggtg ctccggcgcc atcgacaggt
      661 agaagttcga gcccaccttg gccttgagct ggcgcatggc cgtcacgagg ttcgtctgca
      721 gcgcggtgtt ctgcgacacg ccggtctcga ggtcgaggtc gatgccgtcg aagccgtact
      781 tctggatgat ggcgtaggcg ctgttgacga agttcgtcac catcgtcgcg ttgtccagcg
      841 agatcgagcc gttctggccg cccaccgaca gcaccacctt cttgcccgcc ttgcgcttgg
      901 cggcgacgtc ggcgatgaac tgcgcgtcgc tgcccgcgtt cgggtcgacg gtgaagctga
      961 tgttgccgtt gccggcgttg tcggcgaacg agacgatgat cgtgtcccag ttgttcgaca
     1021 cctgcgagat cgggaacgtc gcgcctgacg ggttggtgaa gttgtgccag tagccgacca
     1081 gccagtggcc cgacggcggc ggcgccgggg tgggcgacgg cggcggcgtg ggcgtgggag
     1141 gcggcggcgc cggggtcggc ggcgggggcg ccggcgtggg cggcggcggt gcgggggtcg
     1201 gcggcggcgg ggccggcgtc ggaggcggcg gcgtcgtgcc gcagctggtg atctgggtcc
     1261 acggctggcc ggagccggcg ccgccgttgt tggtggccgg gtcctggcct tgcgtccacc
     1321 agttggcctt gtagctcacg ccgttgcgca ccgcggtgtc gccgccgaca taggcgatcg
     1381 acgacaccca gctctggcat tgcgggttgg ccgccagtgc ggcctgcggc gccagcgcgg
     1441 tgccggccgc cacggcggcg gtcagcaccg ccaggcgctg cagcgtgctc gcgcgggatt
     1501 cgggacgggt catgtcctgc tcctcgtttg gaagggttgg gggtgacgtg ccgacggccg
     1561 gatcgcggga ggcgggccgg ccgtcggtgg gatcgcgtcg gacgcgatcg gggggtcagg
     1621 cgatcagagg cccagcgcgc tgatgaacgc gttggtgaag ccccaggtgc ccgccgtgcc
     1681 gtagctgttg caggtggccg aggcgtagcc gggcgcgcag tccttgtcgc ggtcgagcga
     1741 ccagaagtgg atgcccgagg cgccgttggc cttgacccag ttgctcagcg tggtgacgtc
     1801 ggcgatcgag aaggtctcgt ccgtcgcgtc gttgccgccg atcatcgggg tgatctcgat
     1861 gttcgagtac ggcacgcccc agtagttgtg caggttcacc gccgactgga tcgccgactt
     1921 gcccatgtcg cacttgccgc tggagttgag cgtgcagttc gaggcgatcg cgctgccgta
     1981 gtccatggcc atcaggttga tgatgtagcc ggtcaggccg tgcgacttga tcgagttcat
     2041 caccgtcacg cccatcgagc ccaggctctg cggcgagttg ccgccgagcg tggccagcgt
     2101 gaagctccag cgcaggccgg ggtgcttgga ctgcgaggcc ttgacgcggg cgaccaggtt
     2161 gtcgatgtcg gcggacgtct ggccggcctc gatgtcgaag tcgatgcccg cgaggttggc
     2221 cgacgcatag cggttgacga aggtctcgaa gtcggtgtcg ctcgcgcagg tgaacgaacc
     2281 cgcggcgccg cccgtcgaga tgatgtacct gacgccggcg ttgacgaagt tctgcacgtt
     2341 cgccgaggcg accgcggagg cctgcaggcc gccccagttc tcgctgccgc atgcgccggt
     2401 ggcgaacgcc caggtgatca tcttgagctt ggccggcttg acgctgagca ccggcgacag
     2461 cgtgcccgtc accgccgtcg agatgacgtt ggtgttccag ttcatgctga cggtgatgtc
     2521 cttgtacggg ccgtacatga cgccccccga gggcgggggc gacggcggcg tgggcgtcgg
     2581 g
//
LOCUS       DATCRO010000577         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_659240, whole genome shotgun sequence.
ACCESSION   DATCRO010000577 DATCRO010000000
VERSION     DATCRO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659240"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VIN75_15835"
     CDS             <1..171
                     /locus_tag="VIN75_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975690.1"
                     /translation="GVTILLVEQNARAALEVADHGYVLEMGSVSLEGEAKKLAVDSRV
                     IDTYLGAARKAA"
     gene            218..1963
                     /locus_tag="VIN75_15840"
     CDS             218..1963
                     /locus_tag="VIN75_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY8975691.1"
                     /translation="MKKPQRRFHPALCATALGAAALLAACASRSTAPPAPHAPLAAAR
                     PGTLQACTDLAARAVLPGTVYTSATEVPAGTVTVGGVAMAAPAHCLVQGRMNERTSPV
                     DGQRYAIGFEMRLPVAWNGRFFYQANGGLDGVVVPALGGIGGGGPRSSALQMGFAVIS
                     SDAGHAGSMGPRFGLDPQARLDYGYNAVAQLTPMAKNLIAKAYGRGPDRSYIGGCSNG
                     GRHAMVAAVRSAGQYDGVLAGDPGFNLPKAAVAQLYGAQQYAAVASAKTPAGQPDLQS
                     AFTPAELKLVADKVLQRCDALDGIADGIVSNVNACRTRFDIQRDVPTCAAGTRDGMCL
                     SAPQKNALANVFKGARNGAGQLIYSSFPFDAGVSGANWREWKFASPPTRDAGAVGFIF
                     STPPLADRPQGLPFALGFDMDRDAPRIAATDGVFRESALSFMTPPDAANLGALRERGG
                     RMIVYHGTSDPVFSSDDTQAWYERVQAANGGDASSFARYFPVPGMNHCAGGPATDQFD
                     MLSALVDWVEQGKAPASVTASARGAGTPVLNAEVPAGWAAQRTRPLCPYPQVATYTGG
                     DAERAGSFACKLPTP"
BASE COUNT          296 a          776 c          696 g          253 t
ORIGIN      
        1 ggcgtgacca tcctgctggt ggagcagaac gcgcgcgccg cgctcgaagt ggcggaccac
       61 ggctacgtgc tcgagatggg cagcgtcagc ctggagggcg aggcgaagaa gctggccgtc
      121 gactcgcgcg tgatcgacac ctacctcggc gccgcgagaa aagccgcctg agccaacgac
      181 aacgccgcga cccgcgcggc agcaaggaga ccacagcatg aagaagccac agcgccgctt
      241 ccatccagcc ctgtgcgcga ccgcgctcgg cgccgccgcg ctgctcgcgg cctgcgcgag
      301 ccggtccacc gcgcctccgg ctccccatgc gccgctggcc gccgcgcgcc ccggcacgct
      361 gcaggcctgc accgacctcg ccgcccgcgc ggtgctgccc ggcaccgtct acacatcggc
      421 caccgaggtg ccggccggca ccgtcacggt cggcggcgtc gcgatggcgg cgcccgcgca
      481 ctgcctcgtg cagggccgca tgaacgagcg cacaagcccc gtcgacggcc agcgctatgc
      541 catcggcttc gagatgcggt tgccggtcgc ctggaacggc cgattcttct accaggccaa
      601 cggcgggctc gacggcgtgg tggtgcccgc gctcggcggc atcggcggcg gcggaccgcg
      661 cagcagcgcg ctgcagatgg gcttcgccgt catcagctcc gacgccggcc acgcgggctc
      721 gatgggcccg cgcttcgggc tcgatccgca ggcgcggctg gactacggct acaacgccgt
      781 cgcgcagctc acgccgatgg ccaagaacct gatcgccaag gcctacggcc gcggccccga
      841 ccgctcgtac atcggcggct gctccaacgg cggccggcac gcgatggtgg ccgccgtgcg
      901 ctcggccggc cagtacgacg gcgtgctcgc gggcgacccc ggcttcaacc tgccgaaggc
      961 ggcggtggcg cagctctacg gcgcgcagca gtacgcggcg gtggccagtg cgaagacgcc
     1021 cgccggacag cccgacctgc agagcgcctt cacgcccgcc gagctgaagc tggtggccga
     1081 caaggtgctg cagcgctgcg acgcgctcga cggcatcgcc gacggcatcg tgtcgaacgt
     1141 caacgcctgc cgcacgcgct tcgacatcca gcgcgacgtg ccgacctgcg ccgcgggcac
     1201 gcgcgacggc atgtgcctga gcgcgccgca gaagaacgcg ctcgccaacg tgttcaaggg
     1261 cgcgcgcaac ggcgcgggcc agctcatcta cagcagcttc ccgttcgatg cgggcgtgag
     1321 cggcgccaac tggcgcgagt ggaagttcgc gagcccgccg acgcgcgacg cgggcgcggt
     1381 cggcttcatc ttcagcacgc cgccattggc cgaccggccg cagggcctgc ccttcgcgct
     1441 gggcttcgac atggaccgcg acgcgccgcg catcgccgcc accgacggcg tgttccgcga
     1501 atcggcgctg tcgttcatga cgccgcccga cgctgccaac ctgggcgcac tgcgcgagcg
     1561 cggcggccgc atgatcgtct accacggcac cagcgacccg gtgttctcct ccgacgacac
     1621 gcaggcctgg tatgagcgcg tgcaggccgc gaacggcggc gatgcctcct cgttcgcgcg
     1681 ctacttcccc gtgcccggca tgaaccattg cgcaggcggc ccggccaccg accagttcga
     1741 catgctgagc gcgctggtcg actgggtcga gcagggcaag gcgcccgcat ccgttaccgc
     1801 ctcggcacgc ggcgcgggca cgccggtgct gaatgccgaa gtgcctgccg gctgggccgc
     1861 gcaacgcacg cggccgctgt gcccctatcc gcaggtcgcc acctacaccg gcggcgatgc
     1921 cgagcgcgca ggcagcttcg cttgcaagct gcccacgccc tgatacctag ccagttaccg
     1981 agcctccgat ggattaccag ccccgccccg atgacgtccg c
//
LOCUS       DATCRO010000578         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_118883, whole genome shotgun sequence.
ACCESSION   DATCRO010000578 DATCRO010000000
VERSION     DATCRO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118883"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VIN75_15845"
     CDS             <1..1218
                     /locus_tag="VIN75_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY8975692.1"
                     /translation="SVATIQMINNLLLLRGNIGRPGAGPCPVRGHSNVQGDRTMGIWE
                     KPPAALLDRLRDVFGFEPPRAHGVDTVEAIKAMIEGRGKVFIALGGNFAAATPDTYET
                     WRGLRQCDLTVHVATKLNRSHLVHGREALILPCLGRTEIDVQAAGPQGVTVEDSMSMV
                     HISLGINPPASEHLLSEPAIVARMAAATLGTRSTTPWLWLVEDYDRIRDKIEAVFDDF
                     FDFNERIRVPGGFRLRNTASERVWATAPGKAMFCTHAVPQDTPVHRARARQLDKVVFT
                     LLTTRSHDQYNTTIYGMDDRYRGVFGQRRVVFINPADIRHLGLKDGDWVDLHTAWSDG
                     VERRADRFKLVAYDIPRGNVAAYYPETNPLVPLSSTAIAAGTPTSKSIPVILTAHEAA
                     GSTQQADTTFAGA"
     gene            1282..1560
                     /locus_tag="VIN75_15850"
     CDS             1282..1560
                     /locus_tag="VIN75_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975693.1"
                     /translation="MNDEVPELDELSAAILRELAAAPGEAGMSLPRLGKHLGLGASVL
                     MRSLSAMGNARIGGVDGPGWVRVTQVEERWTAALTELGRAFCARHLGP"
     gene            1655..>4110
                     /gene="mprF"
                     /locus_tag="VIN75_15855"
     CDS             1655..>4110
                     /gene="mprF"
                     /locus_tag="VIN75_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEY8975694.1"
                     /translation="MTQTDSPAPGSTTRAGTDLGEWWARLRPWLLAVVGVALAALVGV
                     ALRRLLDEVHYAGVMAAMDATPAPRIWLAVLATAFSYLALSRYDASALRHVGAKVGEP
                     TILFTSFVAYALSNTIGLGPLSGGVVRMRLYSAAGVEPPQIARVIVFNAAAFGLGMMF
                     FGAIGLLWGAHDVAALLHTRGWLVRLLAAAMLLLLIGFLWLCVRRRTVQFGRVLRMRL
                     PPAELAMRQLGISAIELAASATVLWLLLPAGGIPLPAFVGFYAIAVTAGIVSHVPGGV
                     GVFETVMLLAAGNGVPRDAMLGALLLNRGIYYVLPLVVAAGMMVVYELRSGVVAPVGR
                     MAVRLSPSLMAALTLIAGAWLIVSGATPFTQDARDVLIALHVPVPVVEASHFLGSVAG
                     LGLVLVARGLQHRLAVAWWIASALALVAAALALPKGIALHEAAMLLSLAALLAVSRRQ
                     FDRRSSLLAQHLEPEWIVAIGGVVIACAWVLFFAYKEVAYSNRLWWQFEIDGQAPRSL
                     RALTAVALLALGYGLWQLLRPPAGALAPVSAEEIERAAAIIRANPSTDACYALVGDKH
                     LMFSPSGRSFLAFGKRGRSWVALFGPFGDAREFADIVWQFIEMATDHGGRAAFYHVRP
                     ATLPLLLDCGLHLFKLGEHAQVELASFDLKGASRANLRTAVNRAEREGLRFEVVPAAE
                     VGALLPALREVSDAWLAEHRSGEKAFSVGRFDDDYLRRFDVAVVRRDDRVIAFANLLT
                     TDVREEAALDLMRHLPDAPPGTMDFLLVRILQHLQAQGYRRFGLGMSPMAGMAQRSGA
                     PKWQRVARLVYEHGNGFYNFR"
BASE COUNT          517 a         1512 c         1451 g          630 t
ORIGIN      
        1 tcggtggcga cgatccagat gatcaacaac ctgctgctgc tgcgcggcaa catcggccgg
       61 cccggcgccg gtccgtgccc ggtgcgcggc cacagcaacg tgcagggcga ccgcacgatg
      121 ggcatctggg agaagccgcc cgccgccctg ctcgaccgcc tgcgcgacgt gttcggcttc
      181 gagccgccgc gcgcccacgg cgtcgacacc gtcgaggcca tcaaggcgat gatcgagggc
      241 cgcggcaagg tcttcatcgc cctgggcggc aacttcgccg ccgccacgcc cgacacctac
      301 gagacctggc gcggcctgcg ccagtgcgat ctcacggtgc acgtggccac caagctgaac
      361 cgcagccacc tcgtgcacgg gcgcgaggcg ctgatcctgc cctgcctggg ccgcaccgag
      421 atcgacgtgc aggccgccgg cccgcagggc gtgacggtgg aggactcgat gtcgatggtg
      481 cacatctcgc tgggcatcaa cccgccggcc tccgagcacc tgctgtccga gcccgcgatc
      541 gtcgcccgca tggccgctgc caccctcggc acgcgctcga ccacgccgtg gctgtggctg
      601 gtggaggact acgaccgcat ccgcgacaag atcgaggccg tcttcgacga cttctttgac
      661 ttcaacgagc gcatccgcgt ccccggcggc ttccgcctgc gcaacaccgc gagcgaacgc
      721 gtctgggcca ccgcgccggg caaggcgatg ttctgcacgc acgccgtgcc gcaggacacg
      781 ccggtgcacc gcgcccgtgc gcgccagctc gacaaggtcg tcttcacgct gctgaccacg
      841 cgctcgcacg accagtacaa cacgacgatc tacggcatgg acgaccgcta ccgcggcgtg
      901 ttcggccagc gccgcgtcgt gttcatcaac ccggccgaca tccggcacct gggcctgaag
      961 gacggcgact gggtcgacct gcacacggcc tggagcgacg gcgtcgagcg ccgcgccgac
     1021 cgcttcaagc tggtggccta cgacatcccg cgcggcaacg tcgcggcgta ctaccccgag
     1081 acgaacccgc tcgtgccgct gtcgtccacg gcgatcgcgg ccggcacgcc cacctcgaag
     1141 tcgattcccg tcatactcac ggcacacgag gccgcgggat ccactcagca ggccgatacg
     1201 acgttcgcgg gcgcgtgagc ctcggcgggc cgccgcccgc ctccccccgc gcgacgcgca
     1261 cgcgactgcg cgagagccac gatgaacgac gaggttcccg aactcgacga gctgtcggcc
     1321 gccatcctgc gcgagctggc agccgcgccg ggcgaggccg gcatgtcgct gccacgtctc
     1381 ggcaagcacc tgggtctcgg tgcgagcgtg ctgatgcgct cgctgtcggc gatgggcaac
     1441 gcgcgcatcg gcggcgtcga cggacccggc tgggtgcgcg tcacgcaggt cgaggagcgc
     1501 tggaccgcgg cgctcaccga gctgggacgg gcgttctgcg cgcgccacct cggcccctga
     1561 agcgttcgcg gccgcgcggc gacgccgtcc cggtgccggg tcgacatcgg acctctgttc
     1621 aaatacggcc tcccttggcg cagccgacct tgcagtgacc cagacagact ccccggcgcc
     1681 cggctcgacc acgcgcgcag gcaccgacct cggcgaatgg tgggcgcgac tgcgtccatg
     1741 gctgctggcg gtcgtcgggg tcgcgctggc ggcgctggtc ggcgtcgcgc tgcgccgcct
     1801 gctcgacgag gtgcactacg cgggcgtgat ggcggcgatg gatgcgacgc ccgcgccgcg
     1861 catctggctg gcggtgctgg ccacggcgtt cagctacctc gccctcagcc gctacgacgc
     1921 ctccgcgctg cgccatgtcg gtgcgaaggt cggcgagccc accatcctgt tcacctcgtt
     1981 cgtggcctac gcgctgtcca acaccatcgg gctgggcccg ctgtcgggcg gcgtcgtgcg
     2041 catgcggctg tactcggccg ccggcgtcga gccgccgcag atcgcgcgcg tcatcgtgtt
     2101 caatgccgcg gcgttcggcc tgggcatgat gttcttcggc gccatcggac tgctgtgggg
     2161 cgcacacgac gtcgccgcgc tgctgcacac gcgcggatgg ctggtccgcc tgctggcggc
     2221 ggccatgctg ctgctgctca tcggcttcct gtggctgtgc gtgcggcggc gcaccgtgca
     2281 gttcgggcgg gtcctgcgaa tgcggctgcc accggcggaa ctggcgatgc gccagctggg
     2341 catctcggcg atcgagctgg cggcgtccgc aaccgtgctg tggctgctgc tgcccgcggg
     2401 cggcatccct ctgcccgcgt tcgtcggctt ctacgccatc gcggtgaccg ccggcatcgt
     2461 cagccacgtc ccgggtggcg tcggcgtgtt cgagacggtg atgctgctgg cggccggcaa
     2521 cggcgtgccg cgtgacgcga tgctgggcgc gctgctgctc aatcgcggca tctactacgt
     2581 gctgccgctg gtcgtcgcgg ccggcatgat ggtcgtctac gagctgcgct cgggcgtcgt
     2641 ggcgccggtg ggccgcatgg ccgtgcgcct gagcccgagc ctgatggcgg cgctgaccct
     2701 gatcgccggc gcctggctga tcgtctccgg cgccacgcct ttcacgcagg acgcccgcga
     2761 cgtcctcatc gcgctgcacg tgcccgtgcc ggtggtcgag gcctcgcact tcctgggcag
     2821 cgtggcgggc ctgggactgg tgctggtggc gcgcggcctg cagcaccggc tcgccgtcgc
     2881 ctggtggatc gcgtccgcgc tggccctcgt cgcggcggcg ctggcgctgc ccaagggcat
     2941 cgcgctgcac gaggcggcga tgctgctgtc gctcgcggcg ctgctggcgg tctcgcggcg
     3001 ccagttcgac cggcgctcgt cgctgctggc gcaacacctc gaacccgagt ggatcgtggc
     3061 catcggcggc gtggtcatcg cgtgcgcctg ggtgctgttc ttcgcctaca aggaagtcgc
     3121 ctacagcaac cggctgtggt ggcagttcga gatcgacggc caggcgccgc ggtccctgcg
     3181 cgcgctgacg gcggtggccc tgctcgcgct cggctacggc ctgtggcagc tgctgcggcc
     3241 gccagccggc gcgctggcgc cggtgagcgc cgaggagatc gagcgcgcgg ccgccatcat
     3301 ccgggccaat ccgtccacgg acgcctgcta cgcgctggtc ggcgacaagc acctcatgtt
     3361 ctcgccgtcg ggccgcagct tcctcgcgtt cggcaagcgc ggacgctcct gggtcgccct
     3421 gttcggcccc ttcggcgacg ctcgcgagtt cgccgacatc gtgtggcaat tcatcgagat
     3481 ggccaccgac cacggcggcc gggcggcgtt ctatcacgtg cggccggcga cgctgccgct
     3541 gctgctggac tgcggcctgc acctgttcaa gctcggcgag cacgcgcagg tggaactcgc
     3601 gtcgttcgac ctcaagggcg ccagtcgcgc gaacctgcgc accgccgtca accgggccga
     3661 gcgcgagggc ctgcgcttcg aggtcgtccc ggccgccgag gtcggcgccc tgctgcccgc
     3721 gctgcgcgag gtgtcggacg cctggctcgc cgagcatcgc agcggcgaaa aggccttctc
     3781 cgtcggacgc ttcgacgacg actacctgcg gcgattcgac gtggcggtcg tgcggcgcga
     3841 cgatcgcgtc atcgcgttcg cgaacctgct gacgacggac gtccgcgagg aagcggcgct
     3901 cgacctgatg cgccacctgc cggacgcgcc gcccgggacg atggacttcc tgctggtgcg
     3961 catcctgcag cacctgcagg cgcagggcta ccgccgcttc ggcctgggca tgtcgccgat
     4021 ggccggcatg gcccagcgca gcggcgcgcc gaaatggcag cgcgtcgcgc gactcgtgta
     4081 cgagcacggc aacgggttct acaacttccg
//
LOCUS       DATCRO010000579         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_265401, whole genome shotgun sequence.
ACCESSION   DATCRO010000579 DATCRO010000000
VERSION     DATCRO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265401"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VIN75_15860"
     CDS             complement(<1..246)
                     /locus_tag="VIN75_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8975695.1"
                     /translation="MSVTLEAAPAPPTIAADLVAGVSMAGLLLPEAVAYSGIAGMPPQ
                     AGVLALFAGLLAYGLIGRSRFAIVSATSSSAAVLGAAT"
     gene            complement(243..881)
                     /locus_tag="VIN75_15865"
     CDS             complement(243..881)
                     /locus_tag="VIN75_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEY8975696.1"
                     /translation="MQPAAHHHFIEAELLELHPTQMTAGFAEVALKRREWAGLSKKER
                     KELLASHWFPAVLGPRGRSYIVDHHHLGLALLEEGVERVKLMVLEDLSYLAPTIFWRV
                     MEQRNWVHPFDANGKRQPYTGIPARITELADDPHRSLAGLLRSTGGFAKDASPFAEFL
                     WADYLREHISRGQIRKAIDVALREAQGLAKSPLARYLPGWSGVMPAPAGRRK"
     gene            complement(1052..1282)
                     /locus_tag="VIN75_15870"
     CDS             complement(1052..1282)
                     /locus_tag="VIN75_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975697.1"
                     /translation="MQHQPTSASAARVEWELLPSLMNRLGGRRTRSQPAWMETMPSEI
                     DLLEPSDPFEEVLPGLQMREVREPDIFQIFFG"
     gene            1787..>2292
                     /locus_tag="VIN75_15875"
     CDS             1787..>2292
                     /locus_tag="VIN75_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975698.1"
                     /translation="MKRRIVPFLSAGPLPEWWEPLFEASGNDSVFLSPAWMSHWIDVY
                     GADFTGSWVHWEAGGRVVAGCLLVERVIRVKGVPFRSQFLNATGKAGSPTPLAEFNDV
                     LHLPGHADAVAADFVHLLQERSWGRLLLSGHEHGGVVGRALAHVGGTQTEQRRQPSPF
                     VDLRALGD"
BASE COUNT          320 a          804 c          829 g          339 t
ORIGIN      
        1 cgtcgcggcg cccagcaccg cggccgacga cgacgtcgcc gacacgatcg cgaagcgcga
       61 gcgcccgatc agcccatacg cgagcagccc cgcgaacagg gccagcacgc cggcctgcgg
      121 cggcatgccc gcgatgcccg aatacgcgac ggcctcgggc agcagcaggc cggccatcga
      181 gacgcccgcc accaggtccg ccgcgatcgt cggcggcgcg ggcgcagcct cgagcgtcac
      241 gctcacttgc ggcgcccggc cggcgccggc atcacgccgc tccagccggg caggtagcgc
      301 gcgagcgggc tcttggccag gccctgcgcc tcgcgcagcg cgacgtcgat cgccttgcgg
      361 atctggccgc gcgagatgtg ttcccgcagg tagtcggccc agaggaattc ggcgaacggc
      421 gaggcgtcct tggcgaaacc gcccgtgctg cgcagcaggc ccgcgaggct gcggtgcggg
      481 tcgtcggcaa gctcggtgat gcgcgccggg atcccggtgt agggctggcg cttgccgttc
      541 gcgtcgaacg ggtgcaccca gttgcgctgc tccatcacgc gccagaagat cgtgggcgcc
      601 aggtacgaca ggtcctccag caccatcagc ttgacccgct ccacgccctc ttcgagcagc
      661 gcgaggccca ggtggtggtg gtcgacgatg tagctgcgcc cgcgcgggcc cagcaccgcg
      721 gggaaccagt gcgacgccag gagctccttg cgctccttct tcgacaagcc cgcccattcg
      781 cggcgcttga gcgcgacctc ggcaaagccg gcggtcatct gggtgggatg cagctcgagc
      841 agctcggctt cgatgaagtg gtggtgcgcg gcgggctgca tggcggcggg ggccgcgcat
      901 cgagcgcggg cccggcggcg tgccccggag agtgcggggc tttcattgtg cggccgttcc
      961 gggccgggcg cgccggggcg ccttgcgacc ggctgacgcg ccggcgcgct gcgtgcgcgc
     1021 gagcgcgtga cgcgctcgcg cgggcgatgg gtcaaccgaa gaagatctgg aagatgtcgg
     1081 gctcgcgcac ttcacgcatc tgcaggcccg gcagcacttc ctcgaacgga tcggagggct
     1141 cgagcaggtc gatctccgac ggcatcgtct ccatccaggc cggctgcgag cgcgtgcggc
     1201 gtccgcccag gcggttcatc agcgacggca ggagttccca ctcgacgcgg gcggcggagg
     1261 cggaagtcgg ttgatgttgc atggcggttc ccggtggact gaggcggacg tgccgtcgat
     1321 gagccgttat cgggaagggt ttggccaagc tgtaaccagt tgctgggttc cgcgtgacat
     1381 ctgcccgaac agcggccccg ccgggcgatt cttcaagacc cggtcaggtt cgaccctcag
     1441 gtcgcgtcgc gaagcgccga ccggggcgcg ctgccaccac gcctgcgggc gcacgctgcg
     1501 tcgccgccag gctcgcggga cgccccggat cgccccccct gcagacgcgt tccggggggt
     1561 ggcgtcgcga tcgcgtgaac gatctctcga aacgtctgcg aagcagccgc ggcgacacgt
     1621 cgagtcacaa agttcagctt tacttcaggc aaaatacaag ctatcaaaaa gctgtcaatc
     1681 gccatgcagt gattgcgccg gggggggtgg cacgctgcgt ccgatgcacg ggacgcgcgt
     1741 cgccgggcgt cctcccggcc ggtcgcgccg ccctttcctt cattcgatga agcgacgcat
     1801 cgtccctttc ctgagcgccg gtccgctgcc cgagtggtgg gagccgctgt tcgaggcctc
     1861 cggcaacgac tcggtcttcc tgtcgccggc ctggatgagc cactggatcg acgtctacgg
     1921 cgccgacttc accggctcgt gggtgcactg ggaggccggc ggccgcgtcg tcgcgggctg
     1981 cctgctggtc gagcgcgtga tccgcgtcaa gggcgtgccg ttccgctcgc agttcctcaa
     2041 cgcgaccggc aaggccggat cccccacgcc gctcgccgaa ttcaacgacg tgctgcacct
     2101 gccgggccac gcggatgcag tcgccgcgga cttcgtgcac ctgctgcagg agcgctcgtg
     2161 gggacggctg ctgctgtccg gccacgagca cggcggcgtc gtcggacgcg cgctggcgca
     2221 cgtgggcggc acgcagacgg aacagcgtcg ccagccgtcg cccttcgtcg atctccgggc
     2281 cctcggcgac ag
//
LOCUS       DATCRO010000580         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_186748, whole genome shotgun sequence.
ACCESSION   DATCRO010000580 DATCRO010000000
VERSION     DATCRO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186748"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1258
                     /locus_tag="VIN75_15880"
     CDS             299..1258
                     /locus_tag="VIN75_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8975699.1"
                     /translation="MTDVRWGILGAAKFAREHMGPALTLAPGGHVAALATSEPAKAEP
                     FRAFAPGLRVHDSFDALLADPAIDAVYIPLPNALHVEWTLKAIRAGKHVLCEKPMAMR
                     AGEFDALIAARDAAGVLAAEAFMIVHHPQWQRARQLLADGAVGRLWRIDGAFSFDNRD
                     KANIRNRADMGGGGLRDIGVYVLGGARFATGLEGTDVQARIQWEDGIDVYAAITAKFG
                     EADYVSFTSIRMHPRQEITFHGDAGVLRLPVPFNARVFGEARLELHRGSETIIERWPA
                     VNHYELQAAAFNRSVRDGTPYACPLEFSRGTQAMIDAAFATAA"
     gene            1794..2000
                     /locus_tag="VIN75_15885"
     CDS             1794..2000
                     /locus_tag="VIN75_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975700.1"
                     /translation="MDAMLGRPELREPRIRAWRQIGSAGLNELLDQLESEFQQDGESP
                     ADNAKAGLKVMQGIQLVPHPSRGA"
     gene            complement(2029..>2482)
                     /locus_tag="VIN75_15890"
     CDS             complement(2029..>2482)
                     /locus_tag="VIN75_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEY8975701.1"
                     /translation="IYRRWGELGDLLADVAVERMRPDADPVDTGSVAGDLLAWVEQYR
                     DEMSSDVGLGMMLDVLSAKGGSLAEGKLPVPCQCAAFTAAQIETIVERGVRRGEPVPE
                     VDAVMDTVVSPIVYRALFGTAPMAPEHAQALVEACMKTASKVKAAASA"
BASE COUNT          374 a          897 c          866 g          345 t
ORIGIN      
        1 gtgcatgcgc gtcgaatcga tctgcagcag gttgccgaag accggcacgc cacgcgggcc
       61 gggcaggtcg tcgaaccggc gcagcgtgga tggcgcggcg accgggggag gtggaggctc
      121 catggacgaa ggcacggcgc gcggccacgc acggttgcaa cggggcgtcg atcttgcctg
      181 agcgccgcac gtggcgccag cgccccggct tgcgcggact tgtcatgtcc gagcgcgcgc
      241 ggcggattgc ctcggggcgc tatgctggcc cgcaatcaat gcgcgtggag gatggcacat
      301 gacggatgtt cgatggggaa tcctgggcgc cgcgaagttc gcgcgcgaac acatggggcc
      361 ggcgctgacg ctggcgccgg gcggccacgt cgccgcgctg gcgacctccg agcccgcgaa
      421 ggccgagccg ttccgcgcgt tcgcgccggg cctgcgcgtg cacgacagct tcgacgccct
      481 gctcgccgac cccgccatcg acgccgtcta catcccgctg cccaacgcgc tgcacgtcga
      541 gtggacgctc aaggcgatcc gcgccggcaa gcacgtgctg tgcgagaagc ccatggcgat
      601 gcgggccggc gagttcgacg cgctgatcgc cgcacgcgac gccgcgggcg tgctggcggc
      661 cgaggcgttc atgatcgtcc accacccgca gtggcagcgc gcgcgccagc tgctggccga
      721 cggcgcggtg ggcaggctgt ggcgcatcga cggcgcgttc tcgttcgaca accgcgacaa
      781 ggccaacatc cgcaaccgcg ccgacatggg cggcgggggc ctgcgcgaca tcggcgtcta
      841 cgtgttgggc ggcgcgcgct ttgccaccgg cctcgagggc acggatgtcc aggcccgcat
      901 ccagtgggag gacggcatcg acgtctacgc cgcgatcacc gcgaagttcg gcgaggcgga
      961 ctacgtcagc ttcacgtcga tccgcatgca cccgcgccag gagatcacct tccacggcga
     1021 cgccggcgtg ctgcgcctgc cggtgccctt caacgcccgc gtgttcggcg aggcgcgcct
     1081 ggagctgcat cgaggcagcg agacgatcat cgagcgctgg cccgccgtga accactacga
     1141 gctgcaggcc gccgcgttca accggtcggt gcgtgacggc acgccatacg cctgcccgct
     1201 ggagttctcg cgcggcacgc aggcgatgat cgacgcggcg ttcgcgaccg ccgcctgagt
     1261 ctgcccgccg gccttttccg gccgcatgtc gaaagcggcc cgtgattccc gacatgagtt
     1321 gccgagatct ttcggcgacc aaccaaggac acgcaccacg aaattcccga tgacgctcct
     1381 gcacgtggaa gcggccggcg cagcgatccc gcccgacgag atggccaggt acatggacca
     1441 gatgcacgcc taccgcgccg ccctcagctc ggccgcgcgc gttgacgacg ccctcctcgg
     1501 ccgatcggtc gacaacggcg gtggcatcgg cgcggaggcg acgcgcagac ccgccgccgc
     1561 gcgatttcgg ccgcatacgg tcaccggtat atcgaccgcg cgcccggcag ccccggggga
     1621 tcccgcccgc ctgtcgtgca ggtatcgacc ttgctgcatc cggcgcaccg caatcacacc
     1681 ggcagttcct gctcgtccgg cggaaatgcc gtctagtcat gaattcccag agctccatcc
     1741 tgcgcaaccg gatcaagttc gcggtgcgtc gatgcacagg tcggatcgtc gacatggacg
     1801 ccatgctggg ccggcccgag ctgcgcgagc cgcggatccg cgcgtggcga cagatcggct
     1861 cggcgggact caacgagctg ctcgaccagc tggaatcgga gttccagcag gacggcgaat
     1921 cgccggccga caatgcgaag gccggcctca aggtgatgca gggcatccag ctcgtgccgc
     1981 atccgtcccg cggcgcgtga tcctcgccgc gggtcgcgcc gcagcgtctc aggcggaggc
     2041 cgcggccttg accttgctcg ccgtcttcat gcacgcctcc accagcgcct gcgcgtgttc
     2101 gggcgccatc ggcgcggtgc cgaacagcgc gcggtagacg atcggcgaga cgaccgtgtc
     2161 catcacggcg tcgacctcgg gcaccggctc gccgcgcctg acgccgcgct cgacgatggt
     2221 ctcgatctgc gccgccgtga aggccgcgca ctggcagggc acgggcagct tgccctcggc
     2281 gagcgaaccg cccttggcgg acagcacgtc cagcatcatg cccaggccga cgtccgagga
     2341 catctcgtcg cggtactgct ccacccaggc cagcaggtcg ccggcgacgc tgccggtgtc
     2401 gacgggatcg gcgtcggggc gcatgcgctc caccgcgaca tccgccagca ggtcgccgag
     2461 ctcgccccag cggcggtaga tc
//
LOCUS       DATCRO010000581         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_872544, whole genome shotgun sequence.
ACCESSION   DATCRO010000581 DATCRO010000000
VERSION     DATCRO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872544"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..>2518
                     /locus_tag="VIN75_15895"
     CDS             7..>2518
                     /locus_tag="VIN75_15895"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY8975702.1"
                     /translation="MTTDLQRGLLDAFQPDDGAPLPGAAPLPPQDISLEVLLEKYAKG
                     DERDLHDVRARVARALAQVEAPEQREAWAERFLDALQRGFVPAGRINSAAGTELTATL
                     INCFVQPVGDSIAQEEDGVPGIYTALTEAAETMRRGGGVGYDFSRIRPQGAWVGSTRS
                     RASGPVSYMRVFDRSCETVESAGSRRGAQMAVLRCDHPDIEAFIHAKDQGDLTNFNIS
                     VGVTDAFMKAVDEDTTFDLVHRARPSQEQIDAGARQRADGLWIYRQPPARALWDQIMR
                     CTYDHAEPGVLFLDRINQDNNLSYCEAISATNPCGEQPLPPYGCCCLGSIDLTHFVRD
                     PFEPHASFDHEGFAAVAKTATRMLDNVLDITVWPLPAQLAEANAKRRVGLGFTGLGDA
                     LVMLNLRYDTAAARDAAGRIAQAMRDAAYDASADLAMERGAFPLFNADLYLSRGTFAS
                     RLPGPLKDKIRAQGLRNSHLLSIAPTGTISLAFADNASNGIEPPFSWSYTRKKRMPDG
                     SAKEYAVEDHAWRLYRHLKGVDAPLTDAFVTALEMSAESHAAMVATVAPFVDTSISKT
                     VNVPADYPFADFEGLYRFAWKSGLKGLATYRPNAVLGSVLSTTPASPERLPNAATNET
                     PQPLVATADGGARLADQRLTLDRPATAVMSSLRWPGRPEMNGGNPAWTYMIRHPHGEF
                     ALFVGEMPAQAGPGFGLFAQNVPFEVWVNGAEQPRGLGALAKTLSMDMRVNDPQWLRL
                     KLDVLATVAEERAFDMPFPPAGERRLFPGVVAATAAVIRWRCEQLGALADGGPTPMLD
                     AMFSRDEPRTGTAGTLAWAVDVDNPATGETFTLTMKEVT"
BASE COUNT          378 a          959 c          854 g          327 t
ORIGIN      
        1 tcctcgatga ccaccgacct ccaacgcggc ctgctcgacg ccttccaacc cgacgacggc
       61 gcgccgctgc ccggcgccgc gcccctgccg ccgcaggaca tcagcctcga ggtgctgctc
      121 gagaaatacg ccaagggcga cgagcgcgac ctgcacgacg tgcgcgcgcg tgtcgcgcgc
      181 gcgctcgcgc aggtcgaggc gcccgagcag cgcgaggcgt gggccgagcg cttcctcgac
      241 gcgctgcagc gcggcttcgt gccggcgggc cgcatcaact ccgccgccgg caccgagctc
      301 acggccacgc tgatcaactg cttcgtgcag ccggtgggcg actccatcgc gcaggaggag
      361 gacggcgtgc cgggcatcta caccgcgctc accgaggcgg cggaaaccat gcgccgcggc
      421 ggcggcgtcg gctacgactt ctcgcggatc cgtccgcagg gcgcatgggt gggctccacg
      481 cgcagccgcg cgtcggggcc ggtgagctac atgcgcgtgt tcgaccgcag ctgcgagacc
      541 gtggaatcgg ccggcagccg ccgcggcgcg cagatggccg tgctgcgctg cgaccacccc
      601 gacatcgagg cgttcatcca cgccaaggac cagggcgacc tcaccaactt caacatctcg
      661 gtgggcgtga ccgacgcgtt catgaaggcc gtcgacgagg acacgacctt cgacctcgtc
      721 caccgcgcgc gaccgagcca ggagcagatc gacgccggcg cccgccagcg cgccgacggc
      781 ctgtggatct accgccagcc gcccgcccgc gcgctgtggg accagatcat gcgctgcacc
      841 tacgaccatg ccgagcccgg cgtgctgttc ctggatcgca tcaaccagga caacaacctg
      901 agctattgcg aggcgatcag tgcgacgaac ccgtgcggcg agcagccgct tccgccctac
      961 ggctgctgct gcctcggctc catcgacctg acgcacttcg tgcgcgaccc gttcgagccg
     1021 cacgcgtcgt tcgaccacga gggcttcgcg gccgtcgcca agaccgccac ccgcatgctg
     1081 gacaacgtgc tggacatcac cgtctggccg ctgcccgcgc agctcgccga ggccaacgcc
     1141 aagcgccgcg tgggactcgg cttcacgggc ctgggcgacg cgctcgtcat gctcaacctg
     1201 cgctacgaca ccgccgccgc gcgcgacgcg gcgggccgca tcgcgcaggc gatgcgcgac
     1261 gccgcgtacg acgcgtcggc cgacctcgcg atggagcgcg gcgcgttccc gctgttcaac
     1321 gccgacctgt acctgtcgcg cggcacgttc gcctcgcgcc tgcccggtcc gctgaaggac
     1381 aagatccgcg cgcagggcct gcgcaactcg cacctgctgt cgatcgcacc caccggcacg
     1441 atcagcctgg ccttcgccga caacgcgagc aacggcatcg agccgccgtt ctcgtggtcc
     1501 tacacgcgca agaagcgcat gcccgatggc agcgccaagg agtacgcggt cgaggaccac
     1561 gcgtggcggc tctatcgcca cctcaagggg gtggacgcgc cgctcaccga cgccttcgtc
     1621 accgcgctgg agatgagcgc cgagagccat gccgcgatgg tggccaccgt cgcgcccttc
     1681 gtcgacacgt cgatcagcaa gacggtcaac gtgcccgccg actacccgtt cgccgacttc
     1741 gaggggctgt accgcttcgc gtggaagtcg ggcctgaagg ggctggccac ctaccggccc
     1801 aacgcggtgc tgggctcggt gctgagcacc acgccggcgt cgcccgagcg gctgcccaac
     1861 gcggccacca acgagacgcc gcagccgctc gtggcgaccg ccgacggtgg cgcgcggctc
     1921 gcggaccagc gcctcacgct cgatcgtccc gccaccgcgg tgatgagttc gttgcgctgg
     1981 cccggccggc ccgagatgaa cggcggcaac cccgcgtgga cgtacatgat ccgccacccg
     2041 cacggcgagt tcgcgctgtt cgtcggcgaa atgccggcgc aggccggccc cggcttcggc
     2101 ctgttcgcgc agaacgtgcc gttcgaggtg tgggtcaacg gcgccgagca gccgcgcggc
     2161 ctgggcgcgc tggccaagac gctgtcgatg gacatgcgcg tcaacgaccc gcagtggctc
     2221 aggttgaagc tcgacgtgct ggccacggtc gccgaggagc gcgcgttcga catgccgttc
     2281 ccgcccgccg gcgagcggcg cctgttcccc ggcgtcgtcg ccgcgacggc cgccgtgatc
     2341 cgctggcgct gcgagcagct cggcgcgctg gccgacggcg gcccgacgcc gatgctcgac
     2401 gcgatgttca gccgcgacga gccgcgcacc ggcacggccg gcacgctggc gtgggccgtc
     2461 gacgtcgaca acccggccac cggcgagacc ttcaccctga ccatgaagga agtgacgc
//
LOCUS       DATCRO010000582         9291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_704134, whole genome shotgun sequence.
ACCESSION   DATCRO010000582 DATCRO010000000
VERSION     DATCRO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704134"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1358)
                     /locus_tag="VIN75_15900"
     CDS             complement(3..1358)
                     /locus_tag="VIN75_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8975703.1"
                     /translation="MDNPGLLDIARDVFAAHGPVVAGAKTYAPSDFSVHFFLQLAFIL
                     VACRLVGWLGRKLLGQPQVVGEMIAGVLLGPSLLGLFFPDLQATVFPRETRNVLYAGA
                     QLGVGLYMFLVGTTLRLDHFRSKARAAVGVSIAGIAAPFFIALLITPLLLGIPGLFAA
                     GISQANATLFMGACIALTAFPMLARIVNERGLADTALGTLSLTAGAFDDATSWCVLAL
                     VLATFGAGPGVAILAIGGAVLYGGFVLLFGRRLLAPLGRIVERDGHMSHTVLGIAMAL
                     FCLSAFLMDAIGIHAIFGGFILGVAMPRGLFADELRKKIEPLSVILLLPMFFTYSGLN
                     TRMDMVNSGQLLLVALGILVASVLAKGGACWLAARLSGEDNRTALGIGALMNSRGLME
                     LIIINIGLQKGIIGPALFSMLVLMAIVTTMMASPLFEWVYGRRARENGELGALPGGAP
                     A"
     gene            1532..1780
                     /locus_tag="VIN75_15905"
     CDS             1532..1780
                     /locus_tag="VIN75_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY8975704.1"
                     /translation="MKLLTSREFNRDVSQAKRAARIEPVLITDRGQPTHVLMSIADFR
                     RFSGESENILEILAMPEPVELESGDLSDGAWDHRERIE"
     gene            1780..2280
                     /locus_tag="VIN75_15910"
     CDS             1780..2280
                     /locus_tag="VIN75_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8975705.1"
                     /translation="MFLLDTGVVLELRKARAGQSDPGLAAWAAGIARQKLFISALNLL
                     EIESAAARLGRKDRAAGLVVRQWIDNHVMAAFDGRILPVDAAIVRRRAQLPYANTRDG
                     LLAATALEHGLTLVTRKVAAFRAGRVKLLNPSGVPVQPAAEDEEDWGQATQAGHQWLK
                     NLYVRA"
     gene            2284..3843
                     /locus_tag="VIN75_15915"
     CDS             2284..3843
                     /locus_tag="VIN75_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8975706.1"
                     /translation="MDAQGRTHKRRILIVGGGTAGWLTAAYLARHLVLSERSHLEVTV
                     LESPDIGPIGVGEGTFPTIRATLQFLGIDERRFIRETAATFKQGIRFVDWALPGQEFL
                     HPFEAPFYTEGTSLVPYWLLQDEATRPPFAQAVTLQARVAAAQRAPKRAHEAAYAGPL
                     NYAYHFDALKLARLLAARARELGVQHVEATLSAVTRDERGAIAHVDTLQHGRFQADLY
                     VDCTGFRAELIGQAPGAAFHSVRNHLFADRALTCKLPTGRADAAMESCTIATAHEAGW
                     TWEIGLNGSRGIGCVYSSDHMTDDRAAQILRDYVGPGHEDMATRRIPFTGGWRQKQWV
                     RNCVAVGLSAGFLEPLEATGLVLIEAAVGMIAEMFPHGGPIEAPARRFNELMNARFEN
                     IVNFLKLHYCVSRRDEPFWRDNAALASIPEQLAEFLEQWKYRPPGRYDFLLDTETFAF
                     FNYQYILYGMGYRTDMAAARADFTQVDEAERLFAKIRRFADRALADLPDHGALIRQIN
                     AAAVHALAGAA"
     gene            complement(4165..5325)
                     /locus_tag="VIN75_15920"
     CDS             complement(4165..5325)
                     /locus_tag="VIN75_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975707.1"
                     /translation="MADNTPAPSPLDPTIGTTPVALPTQDACCQSPCDPAWRTKSTCT
                     TFNETRTVILLLGVEDTVAVLARGRIVINVTYAHTMCLVGKQHGGLAYTLTLLPGEKM
                     NLYQSDRYRRTTSETERFSVQTTFAQFVSALHQQASSQDTSALQQVLNSQTSSTSVGA
                     SGGINLGLFSLGGSAGTSSKSSSSSIHSLATQTASSEFVSVAQQASQYTDLQRSITIS
                     SFEDSESVSTTQRTLVNNNRCYAVTYFVRKVVDVYQSATRVVAVTFQVSEGNFVSAVL
                     TPAEIGQLPAALRPAVEAIVKQLPPVGEASQHPTLVTVPTDGVVYDPELAHCCAQDPE
                     LEQAERIRLEREQAEAQRIGLEVQLMALEVQRRQALLAAGTLTPFEPAPPEA"
     gene            5745..7457
                     /locus_tag="VIN75_15925"
     CDS             5745..7457
                     /locus_tag="VIN75_15925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8975708.1"
                     /translation="MKTNANLALSAIALAVSAFTSVQVHAGDGPERSARMAHAQPLAR
                     HVTRHNALLAPAGDPGDDDTAVSLDPSISALCQSTIGQLNVYHPTGQNVDQINGDTIV
                     SAGTQTGCNAAQNETTIAVNPRNPNNLVGGTNDYRIFNSREGRNDGSGWAYTTFDGGR
                     TWRDVQLPHLTFQTGATGALSDMDSAGDPAVAFGNDGTVYYANLVFSRLNDASGIVVS
                     ASHDGGLTWGEPSIVHVDGVDGSGNPVATGIFNDKEWIAVDPRSGAVYVTWTSFQADG
                     SPISVSRSDDGGQTWSAPAFVNPANSFTPGGLTPYSQGSIPQVGNDGTLYIAYEAAIC
                     ATLNCDQPSDHDEVVVATSTDGGQTFTNAAVAVDYDFPYNPDVGRSTLTGENFRMNSY
                     PQMAIDRATNALYVTWADDRHGRYDANGNSIRTNGDVLVVSSRDGRRWSGVQTVGSGA
                     DEFYPAIAAYGGQAAVSFYTRRYDPNGIGLDFAYAQADDGRFGGAGMTRITSQTANPQ
                     GQFVAIGAVTGTVLQGVFIGDYTAAAMGRDRVFHPSWTDFRGHPGVTSPNQDAYAQAI
                     RLDQ"
     gene            complement(7530..>9291)
                     /locus_tag="VIN75_15930"
     CDS             complement(7530..>9291)
                     /locus_tag="VIN75_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8975709.1"
                     /translation="NCAVSALVDARARVVWCCMPRFDSTPVFDALMQSANGVPESGAM
                     ALEIEGLARSEQSYDHGTAILRTRLWDGQGQCVEVVDFAPRFITRDRVFRPAQLVRRI
                     RPLAGRPRVRVTVRPHGGWGAEPPQVTRGSHHVRFVMPEITVRLNTSVPITYLMDGTW
                     FALDREMSLFLGPDETMAGGIDETAREFEDQTSLQWRHWTHRLALPLEYQDAVIRAAI
                     TLKLCQYEETGAIVAAMTTSLPEAPHSGRNWDYRFCWLRDAFFVVRALNGLSEVGTME
                     KYLGWLTNVLHKEAGSGHVQPLYGIGLERELAEAVVPHASGYRGMGPVRIGNQAHEHF
                     QHDTYGNIILASAQSFHDHRLFRRGDASDFAALETMGEQAWRLHDQPDAGIWELRTRA
                     RIHTSSSLMCWAACDRLAHIARRVGMPDRAAFWRERADVIRAKILQFAWNDKRQAYVE
                     SFGGSELDASVLLMGEVGFLPPNDPRFISTVEAMECTLCDGHFMRRYEAADDFGRPET
                     TFNICSFWRVDALARIGRRDEAREAFEALLSRRNPMGILSEDMNPDTGELWGNFPQTY
                     SMVGIIHGALRLSAPWDSVV"
BASE COUNT         1477 a         3246 c         3218 g         1350 t
ORIGIN      
        1 gctcaggccg gcgcgccgcc cggcagcgcg ccgagttcgc cgttctcgcg cgcgcggcgg
       61 ccgtagaccc actcgaacag cgggctggcc atcatcgtcg tgacgatggc catcagcacc
      121 agcatcgaga acagcgccgg accgatgatg cccttctgca ggccgatgtt gatgatgatc
      181 agctccatca ggccgcgcga gttcatcagc gcgccgatgc ccagcgccgt gcggttgtcc
      241 tcgccggaca ggcgcgcggc gagccagcac gcgccgccct tggccagcac cgaggcgacg
      301 aggatgccca gcgccaccag caggagctgg ccggagttga ccatgtccat gcgcgtgttc
      361 aggcccgagt aggtgaagaa catcggcagg agcaggatca ccgacagggg ctcgatcttc
      421 ttgcgcagtt cgtcggcgaa cagcccgcgc ggcatcgcga cgcccaggat gaagccgccg
      481 aagatcgcgt ggatgccgat cgcgtccatc aggaacgccg acaggcagaa cagcgccatc
      541 gcgatgccca gcaccgtatg gctcatgtgg ccgtcgcgtt cgacgatgcg cccaagcggt
      601 gccagcagcc ggcggccgaa cagcaggacg aagccgccgt acaggacggc gccgccgatg
      661 gccaggatcg ccacgccggg gccggcgccg aacgtggcca gcaccagtgc gagcacgcac
      721 caggacgtcg cgtcgtcgaa cgccccggcg gtcagcgaca gcgtgccgag cgccgtgtcg
      781 gccaggccgc gctcgttgac gatgcgcgcc agcatcggaa acgccgtcag cgcgatgcag
      841 gcgcccatga acagcgtggc gttggcttgc gagatcccgg cggcgaacag gcccgggatg
      901 cccagcagca gcggcgtgat cagcagcgcg atgaagaacg gcgcggcgat gccggcgatc
      961 gataccccga cggccgcgcg cgccttggac cggaagtggt ccagccgcag cgtcgtgccg
     1021 accaggaaca tgtacaggcc cacgcccagc tgcgcgccgg cgtacagcac gttgcgcgtc
     1081 tccctgggga acaccgtcgc ctgcaggtcg gggaagaaca ggcccagcaa cgagggcccg
     1141 agcagcacgc ccgcgatcat ctcgcccacc acctgcggct gtcccagcag cttgcggccc
     1201 agccagccga ccaggcggca ggccaccagg atgaaggcca gctgcaggaa gaagtgcacg
     1261 ctgaagtcgc tcggcgcgta cgtcttcgcg cccgcgacca cggggccgtg ggcggcgaac
     1321 acgtcgcgtg cgatgtccag caggccggga ttgtccatgg gaagccctct cttctcgatt
     1381 cgtcggtcac cagaatgaaa gcgatttcat cccttgatgg aatactttgc cagcatgcca
     1441 tgcgttgtcg agccggactg accgatttgc gcggatgtac ctacctggga ttgatatgat
     1501 gaaggcacgt aggtacaagc gggacgccgc catgaagctt ctcacgagcc gcgagttcaa
     1561 ccgcgacgtc agccaggcca agcgcgcggc gcgcatcgag ccggtgctga tcaccgaccg
     1621 cggccagccc acccacgtgc tgatgagcat cgcggacttc cgccgcttct ccggcgagag
     1681 cgagaacatc ctggagatcc tggcgatgcc cgagccggtg gagctcgagt ccggcgacct
     1741 gtccgacggc gcctgggatc accgcgaaag gatcgagtga tgttcctgct ggacaccggc
     1801 gtggtgctgg agctgcgcaa ggcccgggcc gggcagtccg acccgggact ggccgcgtgg
     1861 gcggccggca tcgcgcgcca gaagctgttc atctcggcgc tgaacctgct ggagatcgag
     1921 agcgccgcgg cgcggctcgg gcgcaaggat cgcgcggcgg ggctggtcgt tcggcagtgg
     1981 atcgacaacc acgtgatggc cgccttcgac gggcgcatcc tgcccgtcga cgccgccatc
     2041 gtgcgccggc gcgcgcagct gccgtacgcc aacacgcgcg acggcctgct ggccgccacg
     2101 gcgctggagc acggcctgac gctggtcacg cgcaaggtgg ccgcgttccg cgccgggcgc
     2161 gtcaagctgc tcaacccgtc gggcgtgccg gtgcagccgg cggccgagga cgaggaggac
     2221 tggggccagg ccacgcaggc cggccaccag tggctcaaga acctgtacgt gagggcgtga
     2281 cgcgtggacg cgcaaggacg cacccataag cgccgcatcc tcatcgtggg cggcggcacc
     2341 gccggctggc tgacggcggc ctacctcgca cgccatctcg tgctgtcgga gcgcagccac
     2401 ctcgaggtga cggtgctgga gtcgcccgac atcggcccga tcggcgtcgg cgaaggcacg
     2461 ttccccacga tccgcgcgac gctgcagttc ctgggcatcg acgagcgccg cttcatccgc
     2521 gagacggccg ccacgttcaa gcagggcatc cgcttcgtcg actgggcgct gcccggccag
     2581 gagttcctgc atccgttcga ggcgccgttc tacaccgaag gcacgagcct ggtgccgtac
     2641 tggctgttgc aggacgaggc gacgcgcccg ccattcgcgc aggcggtgac gctgcaggcc
     2701 cgcgtggccg ccgcgcagcg cgcgcccaag cgcgcgcacg aggccgcgta cgccgggccg
     2761 ttgaactacg cctatcactt cgacgcgctc aagctcgcgc ggctgctggc cgcgcgggcg
     2821 cgcgagctgg gcgtgcagca cgtcgaggcg acgctgtcgg ccgtcacccg cgacgagcgc
     2881 ggcgcgatcg cgcacgtcga cacgctgcag cacgggcgct tccaggccga cctgtatgtg
     2941 gactgcacgg gctttcgcgc cgagctgatc ggccaggccc cgggcgccgc gttccactcg
     3001 gtgcgcaacc acctgttcgc cgaccgcgcg ctcacgtgca agctgccgac cgggcgcgcc
     3061 gacgcggcga tggagagctg cacgatcgcc accgcgcacg aggccggctg gacgtgggag
     3121 atcggcctga acggctcgcg cggcatcggc tgcgtctact ccagcgacca catgacggac
     3181 gaccgcgccg cgcagatcct gcgcgactac gtcggccccg gccacgagga catggccacg
     3241 cgccgcatcc cgttcaccgg cggctggcgc cagaagcagt gggtgcgcaa ctgcgtggcg
     3301 gtcggcctgt cggcgggctt cctcgagccg ctggaggcca cgggcctggt gctgatcgag
     3361 gcggcggtcg gcatgatcgc cgagatgttc ccgcacggcg ggccgatcga ggcgcccgcg
     3421 cgccgcttca acgagctgat gaacgcgcgc ttcgagaaca tcgtcaactt cctgaagctg
     3481 cactactgcg tcagccggcg cgacgagccg ttctggcgcg acaacgcggc gctggcgtcc
     3541 atcccggaac agctggccga gttcctcgag cagtggaagt accggccgcc cgggcgctac
     3601 gacttcctgc tggacaccga gaccttcgcg ttcttcaact accagtacat cctgtacggc
     3661 atggggtatc gcaccgacat ggcggccgcg cgcgccgact tcacccaggt cgacgaggcc
     3721 gagcgcctgt tcgccaagat ccgccgcttc gccgaccgtg cgctggcgga cctgccggac
     3781 cacggggcgc tcatccggca gatcaatgcg gcggcggtgc atgcgctggc tggtgctgcg
     3841 tgagcgttga ggtcggcagg gcgaggcgca tcctcgtcac ccgtgcggcg ggcgtgccga
     3901 ttttggtggc ggccagcgga cacagcggac acagggtcag gtcttgattc ttgcggggtc
     3961 aggtcttgaa tcgtgcagtc ccaaggatcc gggcgcctcg tgcctgcggt gctggcgctt
     4021 cggcggccgg cgaaatgcca gcaccgcagg cagacaggca gcccgcatgc aactcgactg
     4081 cacgattcaa gacctgaccc cgcaacgttc aagacctgac cccgcaagaa tcaagacctg
     4141 acccccacgc tgacccccgc gtcgtcaggc ctccggcggc gccggctcga acggcgtcag
     4201 cgtcccggca gccagcaacg cctgccgccg ttgcacctcg agcgccatca gctgcacctc
     4261 gagcccgatc cgctgtgcct cggcctgctc gcgctccagc cggatgcgtt ccgcctgctc
     4321 cagttcggga tcctgcgcgc agcaatgcgc gagctcgggg tcgtagacga cgccgtcggt
     4381 gggcaccgtg acgagcgtcg gatgctggct ggcctcgccc acgggcggca actgcttgac
     4441 gatggcctcg acggccggcc gcagcgccgc cggcagctgc ccgatctccg cgggcgtcag
     4501 caccgccgag acgaagttgc cttcgctgac ctggaacgtg accgccacca cgcgcgtcgc
     4561 gctctggtag acgtcgacga ccttgcgcac gaagtaggtc acggcatagc agcggttgtt
     4621 gttgaccagc gtgcgctgcg tggtggacac gctctcggag tcctcgaagc tgctgatggt
     4681 gatcgagcgc tgcaggtcgg tgtactgcga ggcctgctgc gccaccgaga cgaactcgct
     4741 cgaggcggtc tgcgtggcca gcgagtggat cgacgacgag ctcgacttcg agctggtgcc
     4801 ggcgctgccg cccaggctga acaggcccag gttgatgccg ccgctcgcgc ccacgctggt
     4861 gctcgatgtc tggctgttca acacctgctg cagggcgctg gtgtcctggc tgctggcctg
     4921 ctggtgcagt gccgagacga actgcgcgaa ggtggtctga acggagaagc gctccgtctc
     4981 cgaggtggtg cggcggtagc ggtcgctctg gtacaggttc atcttctcgc cgggcagcag
     5041 ggtcagcgtg tacgcgaggc cgccatgctg cttgccgacc aggcacatcg tgtgcgcgta
     5101 cgtgacgttg atgacgatgc ggccgcgtgc gagcacggcg acggtgtcct cgacgcccag
     5161 cagcaggatg acggtgcgcg tctcgttgaa cgtggtgcac gtcgacttgg tgcgccacgc
     5221 aggatcgcac gggctctggc agcacgcgtc ctgcgtgggc agcgcgacgg gcgtcgtgcc
     5281 gatggtgggg tcgagcggcg agggggcagg ggtgttgtcg gccatggtgg actccttttt
     5341 tcaggacgat cgcagccatg ccgatccctc tggagagatc gcgcaacggg gccgccctgg
     5401 gatgccggcg atgttgctcg gccggtccag tgttggcgtg cgctcgcctg cgttgaatcc
     5461 ctagcggtct gctcaaacgc ggggggcgct cccggctgcc gcgcggggca cgtggatcgt
     5521 catgctgtcc ggcatcgacc cggggggccg cccaccccct agactctcgg ccccttcccg
     5581 cgtgtcgcgg cgggagcgcg ctcccagaat gccggttcca gccggagcgc atcgcctgcg
     5641 tggcatgccg ttccattcga gcgtcgtgcg ccagggcccg gctcgccggt cggcattgcc
     5701 aagcccagcc atgacgtccc catgtccgcg gaggagtcga gctgatgaaa accaacgcca
     5761 accttgccct gtcggcgatc gcactcgctg tgtcagcctt cacgagcgtc caggtccatg
     5821 ccggcgacgg gccggagcgg tcggcgcgca tggcccatgc gcagcccctt gcgcgccacg
     5881 tgacgcgcca caacgcgctg ctcgcgcccg ccggcgatcc cggcgacgac gatacggcgg
     5941 tctcgctgga tccgtcgatc tcggccctgt gccagtcgac catcggccag ctcaacgtct
     6001 accacccgac gggccagaac gtcgatcaga tcaacggcga cacgatcgtc agcgccggca
     6061 cgcagacggg ctgcaacgcc gcgcagaacg agacgacgat cgcggtgaac ccgcgcaacc
     6121 cgaacaatct cgtcggcggc accaacgact acaggatctt caacagccgc gaaggccgca
     6181 acgacggctc gggctgggcc tacacgacgt tcgacggcgg ccgcacctgg cgcgacgtcc
     6241 agctcccgca cctgactttc cagaccggcg cgaccggtgc gctgtccgac atggattccg
     6301 cgggcgaccc ggccgtcgcg ttcggcaacg acggcacggt ttactacgcc aacctcgtgt
     6361 tcagccggct caacgacgcc agcggcatcg tcgtcagcgc ctcgcacgac ggcggcctga
     6421 cctggggcga gccgtccatc gtccatgtgg acggcgtcga cggcagcggc aacccggtgg
     6481 ccaccggcat cttcaacgac aaggaatgga tcgccgtcga tccgcgaagc ggcgcggtct
     6541 acgtcacctg gacgtcgttc caggcggacg gcagcccgat cagcgtctcg cgctccgatg
     6601 acggcgggca gacctggtcg gcgccggcct tcgtgaaccc cgcgaactcg ttcaccccgg
     6661 gcggcctgac gccctattcg cagggttcca tcccgcaggt cgggaacgac ggcacgctct
     6721 acatcgccta cgaggccgcc atttgcgcca cgctgaactg cgaccagccg agcgaccacg
     6781 acgaggtggt cgtggcgaca tcgaccgacg gcggccagac cttcaccaat gccgccgtgg
     6841 cggtcgacta cgacttcccc tacaacccgg acgtcggccg ttccacgctg accggcgaga
     6901 acttccggat gaacagctac ccgcaaatgg cgatcgaccg cgcgaccaac gcgctgtacg
     6961 tcacctgggc cgacgatcgc catgggcgct acgacgcgaa cggcaactcg atcaggacca
     7021 atggcgacgt gctcgtggtt tcgtcgcgcg acggccgtcg ctggtccggc gtgcagacgg
     7081 tgggctccgg cgcggacgag ttctaccccg ccatcgccgc ctacgggggc caggcggcgg
     7141 tgagcttcta cacgcgccgg tacgacccca acggcatcgg tctggacttc gcctatgcgc
     7201 aggcggacga cgggcgattc ggaggcgccg gcatgactcg gatcacgagc cagacagcca
     7261 acccgcaggg gcagttcgtc gccatcggcg ccgtcaccgg caccgtcctg cagggcgtgt
     7321 tcatcggcga ctacacggcc gcggccatgg gccgggaccg cgtgttccat ccgtcctgga
     7381 cggacttccg cggacaccct ggcgtgacct cgccgaacca ggacgcctac gcgcaggcga
     7441 tccgtctcga ccaataggcc gggacggcgg ccgcccgctc gcgcacgccc gcgacgacga
     7501 cccgtcgcgg agcgcgaggc ggcggccggt cacacgaccg agtcccacgg cgccgagagc
     7561 cgcagcgcgc catggatgat gcccaccatc gaataggtct gcgggaagtt gccccacagc
     7621 tcgccggtgt cggggttcat gtcctcgctg aggatgccca tcgggttgcg gcgtgacagc
     7681 agcgcctcga aggcctcgcg cgcctcgtcg cggcggccga tgcgcgccag cgcgtcgacg
     7741 cgccagaacg agcagatgtt gaacgtcgtc tcggggcggc cgaagtcgtc ggcggcctcg
     7801 tagcggcgca tgaagtggcc gtcgcacagc gtgcattcca tcgcctcgac ggtgctgatg
     7861 aagcgcgggt cgttgggcgg caggaagccc acctcgccca tcaacagcac gctggcgtcg
     7921 agctcgctgc cgccgaagct ctcgacgtag gcctggcgct tgtcgttcca ggcgaactgc
     7981 aggatcttgg cgcggatgac gtcggcgcgc tcgcgccaga acgccgcgcg gtccggcatg
     8041 cccacgcggc gcgcgatgtg cgcgagccgg tcgcacgcgg cccagcacat cagcgacgac
     8101 gacgtgtgga tgcgggcgcg cgtgcgcaac tcccagatgc cggcgtcggg ctggtcgtgc
     8161 agccgccagg cctgctcgcc catcgtctcg agcgcggcga agtcggacgc atcgccgcgg
     8221 cggaacaggc gatggtcgtg gaacgactgc gccgaggcga ggatgatgtt gccgtaggtg
     8281 tcgtgctgga agtgctcgtg cgcctggttg ccgatgcgca ccggtcccat gccgcggtag
     8341 ccgctcgcgt gcggcacgac ggcctcggcc agctcgcgct ccaggccgat gccgtacagc
     8401 ggctgcacgt ggcccgagcc ggcctccttg tgcagcacgt tggtcagcca gccgaggtat
     8461 ttttccatcg tgccgacctc ggacagcccg ttgagcgcgc gcaccacgaa gaaggcatcg
     8521 cgcagccagc agaagcggta gtcccagttg cgcccgctgt gcggcgcctc gggcaggctg
     8581 gtcgtcatgg cagcgacgat cgcgcccgtc tcctcgtact ggcacagctt gagcgtgatg
     8641 gccgcgcgga tgacggcgtc ctggtactcg agcggcagcg ccaggcgatg cgtccagtgg
     8701 cgccactgca gcgaggtctg gtcctcgaac tcgcgcgcgg tctcgtcgat gccgccggcc
     8761 atggtctcgt cggggcccag gaacaggctc atctcgcgat cgagcgcgaa ccaggtgccg
     8821 tccatcaggt aggtgatagg cacgctggta ttcagccgga cggtgatctc gggcatgacg
     8881 aagcgcacgt ggtggctgcc gcgagtgacc tgcggcggct ccgcgcccca gccgccatgc
     8941 ggccgaaccg tgacgcgcac gcgcggccgc ccggctaacg gccggatgcg ccggacgagc
     9001 tgcgccgggc ggaacacgcg gtcgcgcgtg atgaagcgcg gcgcgaaatc gaccacctcg
     9061 acgcactggc cttgtccatc ccacaagcgg gtgcgtaaga tggccgtgcc atgatcatag
     9121 gactgctcgc tgcgagcgag gccttcgatt tccaaagcca tggcgccgga ctcgggtaca
     9181 ccgtttgccg actgcatcaa cgcgtcgaag accggtgtac tgtcgaacct cggcatgcag
     9241 caccagacca cgcgggcccg ggcgtcgacc agggcactga cggcgcagtt g
//
LOCUS       DATCRO010000583         9888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_434058, whole genome shotgun sequence.
ACCESSION   DATCRO010000583 DATCRO010000000
VERSION     DATCRO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434058"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VIN75_15935"
     CDS             <1..499
                     /locus_tag="VIN75_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY8975710.1"
                     /translation="ITLDIPASTNAAGAATLPITGIQLDMSGATEFGSNFGVTNMTQD
                     GYTAGQLTGVSVDPKGQLIAQYSNGQTKAAGQVELATFRNVQGLTPLGGNAWKQTAAS
                     GGPTVGVAGDGNLGVLNSGSLEESNVDLTAELVSMITAQRDYQANAQTIKTMDQAMQT
                     LVNLR"
     gene            526..1251
                     /gene="flgF"
                     /locus_tag="VIN75_15940"
     CDS             526..1251
                     /gene="flgF"
                     /locus_tag="VIN75_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEY8975711.1"
                     /translation="MDRMIYLSMSGAKAALQRQDSLANNLANVSTNGFRAELTAFRAV
                     PIRGDGASTRVFSLETTPGYSDEQGAINATGRNLDVAMTGKSWLAVQSLDGTEAYTRA
                     GALDVNAEGVLVTQGGLQVNGDGGPITVPQNARVDIGEDGTVTAKVDGQKPQQIGKLK
                     LVTPDVPMNRGTDGLFRADSGDLAADPAARLQSGALEGSNVSPVATMVGMIAAARQFE
                     HQMKMLENAEKQDQTASKLLSEN"
     gene            1552..2334
                     /gene="flgG"
                     /locus_tag="VIN75_15945"
     CDS             1552..2334
                     /gene="flgG"
                     /locus_tag="VIN75_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY8975712.1"
                     /translation="MLRSLWISKTGMEAQQLQLDNISHNLANVATNGYKKSHAQFEDL
                     IYQNLRQSGSATSDQTQLPNGLQVGLGVRPVATARDFSQGSLQQSGNSLDVAISGNGF
                     LQVTMPDGTTGYTRDGSLKLDSQGQLVTNEGFIVQPGITIPPQAQSISIGADGTVSAT
                     LPGTAQPSTLGQLQLAGFPNPQGLDPKGGNIYAETAASGAPSAAAPGTNGLGRIQQGF
                     VETSNVNVVEELVAMISTQRAYELNSKAIQTSDQMLQRLGQL"
     gene            2424..3518
                     /locus_tag="VIN75_15950"
     CDS             2424..3518
                     /locus_tag="VIN75_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY8975713.1"
                     /translation="MAAIIVAILAALFTTPAHATRIKEVAAVQGVRSNQLVGYGLVIG
                     LDGTGDQTTQAPFTTQGLQSMLQQLGVTLPPGTTMQLKNVAAVIVTASLPAFAQPGQN
                     IDVDVSSVANAKSLRGGTLVATPLKGADGQVYAIAQGNLIVGGAGAAAGGSKVTINHL
                     SAGRIPEGASVERAVPTPLSQGDTLELDLSSADYATARAVANAINVSKGMGTARALDG
                     RVIKVKAPTDSDARVSFLADLENLDINLSAPAAKIVMNARTGSIVMNQAVTLAPCAVA
                     HGSLSVTINSNPQVSQPAPNSGGQTVVTDKTDIAISSQAGSLVNMPAGTKLADVVKAL
                     NALGATPQDLLAILQAMKSAGALTADIEVI"
     gene            3521..4402
                     /gene="flgJ"
                     /locus_tag="VIN75_15955"
     CDS             3521..4402
                     /gene="flgJ"
                     /locus_tag="VIN75_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEY8975714.1"
                     /translation="MTSPISNSIAGAPQSLNLAGGLENLRVKAQQDPKAAVREAAKQF
                     ETMFTQQLLKQMRDSTMNTDMMDNEGTKMGTEMLDSQWASKLAGQPGGLSDIIARQLE
                     RQMGITQGITAKPATANPMPLDPIHPDAPVGIPQKGAAAFIQAHTDAAAAAEKATGIP
                     ARFMIAQAAHETGWGRKEIRNADGSTSFNLFGIKASPNWKGATTDVTTTEYYGGKAHK
                     VTQTFRAYASYAESFADYASTMKNSPRYAAVVAAGNDASGFARGLQKAGYATDPSYAT
                     KLAKMIHMTERVQQTLA"
     gene            4473..6488
                     /gene="flgK"
                     /locus_tag="VIN75_15960"
     CDS             4473..6488
                     /gene="flgK"
                     /locus_tag="VIN75_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY8975715.1"
                     /translation="MTSSALMSIGMRAMFANYASMQTTGHNIANAQTPGYSRQTVDLA
                     TSGGQQTGAGFFGKGVDVVTVRRASDAFLNTQSQVASSMAAMDDARSSNLQQLETVFP
                     PGDTGMGAAVGTFMNSYVDLANSPADSSARQVILTNAAEVADRFATAGGQLDRLQQGV
                     TQDLRNSVAQVNQLSSQIAKLNYDIAGLQGSGQSPNDLMDQRDQMVKQLGNLVKVSTI
                     TANDGSMGVFIAGGQRLVLGAEASKLAVTPDPANSGRSTISMDDNGIQRELSTDLLVG
                     GSISGLMKYQNEDLVAARNQLGQMAASVAARVNAVQSRGIDMGSPPGAGDAIFAVGGP
                     IAIPNAHNSRDASGGFASQVSLTVTDASQLKASDYTLASDPANGGAYIVTRQSDGVKF
                     SMAPDGGGGFQYTRQSDGAAMGGSMDGFQISFNGTPAAGDTFELEPVGRAATSMVRTL
                     DDPNGIAAASPETAVMGVNNKGTATVAGLSVVDSTNDPTLTAAISFTDASGGYSYTLT
                     DASGNVTASGNGTWTPGQPIAMNGFALSLNGVPAAGDTINVAKTTHPESNNGNALAMV
                     GLRDESFVGRDMQIDGTLADGETATDAYASVLANVGVRVQSAKTASTISAATSEQADQ
                     AVANKSGVNLDEEAGRLIQFQQGYQAAAKVLQVAQAIFDTLLQMAGH"
     gene            6504..7703
                     /gene="flgL"
                     /locus_tag="VIN75_15965"
     CDS             6504..7703
                     /gene="flgL"
                     /locus_tag="VIN75_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY8975716.1"
                     /translation="MSVERISTAHAYDSALNNLMSRQDNLTATQEQMTSGKRVIKASD
                     DPTAAARAERARALEDRTTASEKAVNASANAMTLTESALGDAGELLQQARELLVSAGD
                     ASYSDAERASKADALKSIREQLLSVANRPDGQGGFVFSGQGSGGAPFVDKPGGVAFQG
                     IGGQVNVATDEPLPLTLDGGNVWLSASTGNGVFVTKNTNSSNAWIDAGRVTSPDQLTG
                     STYDIQFSTAGGATTYSVLKDGNPVSSGNAFTSGQNITVDGMSFAISGDPANGDDFQL
                     SPSTRSLSVFDALDNAVKQLSTPDQGGPKVQQAVQDSIRDIDQCSSQISSARSFAGTT
                     LQRLDGVKGRLDATELSAQTSRSNAEDLDMVKALSSFNQQQTGYQAALQSYATIQKLS
                     LFNYLNA"
     gene            7766..8962
                     /locus_tag="VIN75_15970"
     CDS             7766..8962
                     /locus_tag="VIN75_15970"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY8975717.1"
                     /translation="MSLDHPVLDQVVLGASSLFDARRAPIAAHMTVFPQRPDAPIDAA
                     GLLQVVDVIAPPEAGPLMLSVTHEGSLRALLAAPTAAHLMIEVPGFMLSDPGLATLLH
                     ERVAQGLSLVLGGRIPDSAPQDLWQLFQAVTVDRANIRNPLPAGLGGRPLQRFFIGAD
                     GPAMSAGALRAGFSAVVGWPTGDAPGPDTKAKNVPAGVTTVVDLMQRVDRQEPAEKLE
                     AVVKNDPTLAFRLMRYLNSPGFGLSVEISSFRHALMILGYQRLKRWLALLVTSAIDDP
                     DMKPLMQLAVRRGLVMEEIARPGGDDALRNELFICGVFSTLDRMMGQPFEQLLKTIPV
                     QESVAKALAHESGPLAPFLALARAIETESAYDIREAADTLMLTVGDVNRALAKALVHA
                     HSLTKG"
     gene            9124..9630
                     /locus_tag="VIN75_15975"
     CDS             9124..9630
                     /locus_tag="VIN75_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975718.1"
                     /translation="MIRSTRLAAGVVLVALAQIGNVQAQTVHKCIVNGAAVYQAAACP
                     AANEQKSLVIPSAPSQQELLDATANGRLQSVTPGSNVPQVGTSRRYDRHPVVASPELT
                     PSMQDPEQVPTNSCDRLNQTYQDAKYRRDELSAPGGGANRAPALQRAIDDMKRAQDMA
                     ANTQCHLR"
     gene            9697..>9888
                     /locus_tag="VIN75_15980"
     CDS             9697..>9888
                     /locus_tag="VIN75_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8975719.1"
                     /translation="MTRTYGRAASLAALFATVAVHAQEVHKCTVNGTVTYQARPCATG
                     DVVLPPAPMPSDQDTRQAQE"
BASE COUNT         1738 a         3640 c         3264 g         1246 t
ORIGIN      
        1 gatcaccctg gacatccccg cgtcgaccaa cgccgccggc gccgcgacgc tgccgatcac
       61 cggcatccag ctggacatgt ccggcgcgac cgagttcggc tcgaacttcg gcgtcaccaa
      121 catgacccag gacggctaca ccgcgggcca gctcacgggc gtgtccgtcg acccgaaggg
      181 gcagctgatc gcccagtact ccaacggcca gaccaaggcc gccggccagg tcgagctggc
      241 caccttccgc aacgtgcagg gcctgacgcc gctgggcggc aacgcctgga agcagaccgc
      301 cgcgtcgggc ggcccgaccg tcggcgtcgc cggcgacggc aacctgggcg tgctcaattc
      361 cggctcgctg gaggaatcga acgtcgacct gacggcggag ctggtctcga tgatcacggc
      421 gcagcgcgac taccaggcca acgcccagac catcaagacg atggaccagg cgatgcagac
      481 cctcgtgaac ctgcgctgag cggattgagc tgaaggggag cgaccatgga ccgcatgatc
      541 tacctgtcga tgtcgggcgc gaaggcggcc ctgcagcgcc aggattcgct cgccaacaac
      601 ctcgccaacg tctcgaccaa cggtttccgc gccgagctca ccgccttccg cgcggtgccg
      661 atccgcggcg acggcgcgtc cacccgcgtg ttctcgctgg agaccacgcc gggctacagc
      721 gacgagcagg gcgcgatcaa cgccaccggg cgcaacctgg acgtcgcgat gaccggcaag
      781 tcctggctcg cggtgcagtc gctggacggc accgaggcct acacccgtgc cggtgcgctg
      841 gacgtcaatg ccgaaggcgt gctggtcacc cagggcggcc tgcaggtcaa cggcgacggc
      901 ggcccgatca ccgtgccgca gaacgcgcgc gtggacatcg gcgaggacgg cacggtcacc
      961 gccaaggtcg acggccagaa gccgcagcag atcggcaagc tcaagctggt cacgcccgac
     1021 gtgccgatga accgcggcac cgacggcctg ttccgcgccg attccggcga cctcgccgcc
     1081 gaccccgccg cgcgcctgca gtcgggcgcg ctggaaggct ccaacgtgag ccccgtggcg
     1141 acgatggtgg gcatgatcgc cgccgcgcgc cagttcgagc accagatgaa gatgctcgag
     1201 aacgccgaga agcaggacca gacggcgtcc aagctgttga gcgagaactg agggcgccgc
     1261 cgcgccgcaa ggggtctggc ctctccccga atcgcgccgc cgcgcagccg caagcgggga
     1321 cgtccaccgc gatgcgggga caggcctgac cccgctgccg atcgaagccg gggtcaggcc
     1381 tgtcctcgga tcgcgggccg atcctcgccg acgccggcgc aacagcgcga tccgaggaga
     1441 ggccagaccc ctcccagtcg ggataagcgg cccctatgcc ggccttatcc ggtcgatcca
     1501 cccctcaagt tttggggaag atggcgtatc actcgaacga aagttcacgt catgcttcgc
     1561 tcattgtgga tctcgaagac cggcatggag gcccagcagc tccagctgga caacatctcg
     1621 cacaacctcg ccaacgtggc caccaacggc tacaagaaga gccatgcgca gttcgaggac
     1681 ctgatctacc agaacctgcg ccagtccggc tccgcgacgt ccgaccagac gcagctgccc
     1741 aacggcctgc aggtcggcct gggcgtgcgc ccggtggcga cggcgcgtga cttctcgcaa
     1801 ggctcgctgc agcagtccgg caactcgctg gacgtggcca tcagcggcaa cggcttcctg
     1861 caggtgacga tgcccgacgg caccaccggc tacacgcgcg acggctcgct caagctcgac
     1921 tcgcaaggcc agctggtcac caacgagggc ttcatcgtgc agcccggcat cacgatcccg
     1981 ccgcaggccc agagcatctc gatcggcgcc gacggcaccg tctcggccac gctgcccggc
     2041 accgcgcagc ccagcacgct gggccagctg cagctcgccg gatttcccaa cccgcagggc
     2101 ctggacccca agggcggcaa catctacgcc gagaccgccg cctccggcgc gccgagcgcc
     2161 gccgcgccgg gcaccaatgg cctgggccgc atccagcagg gcttcgtcga gacctccaac
     2221 gtcaacgtcg tcgaggaact ggtggcgatg atctccaccc agcgcgccta cgagctcaac
     2281 tccaaggcga tccagacctc cgaccagatg ctgcaacgcc tgggacaact ctgatgctcg
     2341 actccgcttc aggcaaccgc catctccgcc atcgcgtccc gcacacgcgc aacgtgtcgg
     2401 accgcgccgc gctgcgcatc ggcatggccg cgatcatcgt ggccatcctc gcggcgctgt
     2461 tcaccacgcc cgcccacgcc acgcgcatca aggaagtggc ggccgtgcag ggcgtgcgct
     2521 ccaaccagct ggtcggctac ggcctggtca tcggcctgga tggcacgggc gaccagacca
     2581 cccaggcccc gttcaccacg cagggcctgc agtcgatgct tcagcagctg ggcgtgacgc
     2641 tgcccccggg caccacgatg cagctcaaga acgtggcggc cgtgatcgtc accgcctcgc
     2701 tgcctgcgtt cgcgcagccg ggccagaaca tcgacgtcga cgtctcgtcg gtggccaacg
     2761 ccaagagcct gcgcggcggc acgctggtgg ccacgcctct gaagggcgcc gacggccagg
     2821 tctacgcgat cgcccagggc aacctgatcg tcggcggcgc cggcgccgcc gcgggcggct
     2881 ccaaggtcac gatcaaccat ctcagcgccg gccgcatccc ggaaggcgcc tcggtcgaac
     2941 gcgcggtgcc cacgcccctg tcgcagggcg acacgctgga gctcgacctg agctcggccg
     3001 actacgccac cgcccgcgcg gtcgccaacg cgatcaacgt gagcaagggc atgggcacgg
     3061 cgcgcgcgct ggacggccgc gtcatcaagg tcaaggcgcc caccgactcc gatgcgcgcg
     3121 tgagcttcct cgcggacctc gagaacctgg acatcaacct gtccgcgccg gccgccaaga
     3181 tcgtgatgaa cgcgcgcacc ggctcgatcg tgatgaacca ggcggtcacc ctcgcgccgt
     3241 gcgccgtcgc gcacggcagc ctgtcggtga cgatcaactc caacccgcag gtgagccagc
     3301 ccgcgccgaa ctcgggcggc cagacggtgg tcaccgacaa gacggacatc gcgatctcgt
     3361 cgcaggccgg ctcgctcgtg aacatgcccg cgggcaccaa gctggccgac gtcgtcaagg
     3421 cgctcaacgc gctcggcgcc acgccgcagg acctgctcgc gatcctgcag gcgatgaagt
     3481 cggccggcgc gctgaccgcg gacatcgagg tcatctgatc atgaccagcc ccatctcgaa
     3541 ttccatcgcg ggcgccccgc agtcgctgaa cctggccggc ggcctggaga acctgcgcgt
     3601 caaggcgcag caggacccga aggccgcggt gcgcgaggcc gccaagcagt tcgagacgat
     3661 gttcacgcag cagttgctca agcagatgcg cgactccacg atgaacaccg acatgatgga
     3721 caacgagggc acgaagatgg gcaccgagat gctcgattcg cagtgggcct ccaagctcgc
     3781 cggccagccg ggcgggctgt cggacatcat cgcgaggcaa ctcgagcgcc agatgggcat
     3841 cacgcagggc atcacggcca agccggccac ggccaacccg atgccgctcg acccgatcca
     3901 cccggatgcc ccggtaggca tcccgcagaa gggcgccgcc gcgttcatcc aggcgcacac
     3961 cgacgccgcc gccgcggccg agaaggccac cggcatcccc gccaggttca tgatcgcgca
     4021 ggccgcgcac gagacgggct ggggccgcaa ggagatcagg aacgccgacg gctcgacctc
     4081 gttcaacctg ttcggcatca aggccagccc gaactggaag ggcgcgacca ccgacgtgac
     4141 gaccaccgag tactacggcg gcaaggcgca caaggtcacg cagaccttcc gcgcctacgc
     4201 gagctacgcc gagtcgttcg ccgactacgc cagcacgatg aagaacagcc cgcgctatgc
     4261 cgcggtcgtg gccgccggca acgacgccag cggcttcgcg cgcggcctgc agaaggccgg
     4321 ctacgccacc gacccgtcgt acgcgacgaa gctggccaag atgatccaca tgaccgagcg
     4381 cgtccagcag acgctggctt gacgcgtgca cgaccacacc ccgcgcccat cgtgatctcg
     4441 accgcacact tcgcaactga aaggagcgcc tcatgacgtc gtcggccctg atgtcgatcg
     4501 gcatgcgcgc catgttcgcg aactatgcgt cgatgcagac gaccggtcac aacatcgcga
     4561 acgcgcagac gcccggctac tcgcgccaga ccgtcgacct ggccacctcc ggcggccagc
     4621 agaccggcgc gggcttcttc ggcaagggcg tggacgtcgt cacggtgcgg cgcgcgagcg
     4681 acgccttcct gaacacgcag tcgcaggtgg ccagctcgat ggccgcgatg gacgacgcac
     4741 gatcgtccaa cctgcagcag ctcgagacgg tgttcccgcc cggcgacacc ggcatgggcg
     4801 ccgccgtcgg cacgttcatg aactcgtacg tcgacctggc caacagcccg gccgattcgt
     4861 cggcgcgcca ggtgatcctg accaacgccg cggaggtggc cgaccgcttc gccaccgccg
     4921 gcggccagct cgaccgcctg cagcagggcg tgacgcagga cctgcgcaac tcggtggcgc
     4981 aggtcaacca gctgtcgtcg cagatcgcca agctcaatta cgacatcgcc ggcctgcagg
     5041 gctcgggcca gtcgcccaac gacctgatgg accagcgcga ccagatggtc aagcagctgg
     5101 gcaacctggt caaggtcagc accatcacgg ccaacgacgg ctcgatgggc gtgttcatcg
     5161 ccggcggcca gcgcctggtg ctgggcgccg aggcgtcgaa actggccgtg acgcccgacc
     5221 cggccaacag cggccgctcg acgatctcga tggacgacaa cggcatccag cgcgagctct
     5281 cgaccgacct gctggtgggc ggctcgatca gcgggctgat gaagtaccag aacgaggacc
     5341 tcgtcgccgc gcgcaaccag ctgggccaga tggcggcctc ggtggcggcg cgcgtcaacg
     5401 cggtgcagtc gcgcggcatc gacatgggct cgccccccgg cgccggcgac gcgatcttcg
     5461 cggtcggcgg cccgatcgcg atccccaacg cgcacaactc gcgcgacgcc tccggcggct
     5521 tcgcgtccca ggtgtcgctg accgtcaccg acgcctcgca gctcaaggcg agcgactaca
     5581 cgctggcgtc cgatccggcc aacggcggcg cgtacatcgt cacgcgccag tccgacggcg
     5641 tgaagttctc gatggcgccg gacggcggcg gcggcttcca gtacacgcgc cagtccgacg
     5701 gcgcggcgat gggcggcagc atggacggct tccagatctc gttcaacggc acgcccgcgg
     5761 ccggcgacac cttcgagctc gagcccgtgg gccgcgccgc gacgagcatg gtgcgcaccc
     5821 tcgacgatcc gaacggcatc gccgcggcct cgccggagac ggccgtcatg ggcgtcaaca
     5881 acaagggcac ggccaccgtc gccggcctga gcgtggtcga ctcgaccaac gacccgaccc
     5941 tgaccgcggc catcagcttc accgacgcga gcggcggcta ctcgtacacg ctgaccgacg
     6001 cgagcggcaa cgtcaccgcc tcgggcaacg gcacctggac gccgggccag ccgatcgcga
     6061 tgaacggctt cgcgctgagc ctcaacggcg tgccggccgc gggcgacacc atcaacgtgg
     6121 ccaagacgac gcaccccgag agcaacaacg gcaacgcgct ggcgatggtc gggctgcgcg
     6181 acgagagctt cgtgggccgc gacatgcaga tcgacggcac gctggccgac ggcgagaccg
     6241 ccaccgacgc ctacgcgagc gtgctggcca acgtcggcgt gcgcgtgcag agcgccaaga
     6301 cggcgtcgac gatctcggcc gccacgtccg agcaggccga ccaggcggtc gccaacaaga
     6361 gcggcgtcaa cctcgacgag gaggccggcc gcctgatcca gttccagcag ggctaccagg
     6421 ccgccgccaa ggtgctgcag gtggcgcagg ccatcttcga caccctgctg cagatggccg
     6481 gtcactgata agcaggcgca cgcatgtccg tagaacgcat ctccaccgcc cacgcgtacg
     6541 actccgcgct gaacaacctg atgtcgcgcc aggacaacct gacggcgacg caggagcaga
     6601 tgaccagcgg caagcgcgtc atcaaggcca gcgacgaccc gaccgccgcc gcgcgcgccg
     6661 aacgggcccg cgcgctcgag gatcgcacca ccgccagcga gaaggccgtc aacgccagcg
     6721 ccaacgccat gacgctgacg gagtcggcgc tgggcgacgc cggcgagttg ctgcagcagg
     6781 cgcgcgagct gctcgtgagc gccggcgacg ccagctacag cgacgctgaa cgcgcgtcca
     6841 aggccgatgc gctcaagtcg atccgcgagc agctgctgtc ggtggccaac cggcccgacg
     6901 gccagggcgg cttcgtgttc tcgggccagg gctcgggcgg cgcgcccttc gtcgacaagc
     6961 ccggcggcgt ggcgttccag ggcatcggcg gccaggtcaa cgtggccacc gacgagccgc
     7021 tgccgctgac gctggacggc ggcaacgtct ggctgtcggc cagcaccggc aacggcgtgt
     7081 tcgtcacgaa gaacaccaac tccagcaacg cctggatcga cgccggccgc gtgacctcgc
     7141 ccgaccagct caccggctcg acctacgaca tccagttcag caccgccggc ggcgccacca
     7201 cgtactcggt gctcaaggac ggcaacccgg tctccagcgg caacgccttc accagtggcc
     7261 agaacatcac ggtggacggg atgtccttcg cgatctcggg cgacccggcc aacggcgacg
     7321 acttccagct gtcgccctcg acgcgctcgc tgtcggtgtt cgacgccctg gacaacgcgg
     7381 tcaagcagtt gtccacgccc gaccagggcg gcccgaaggt gcaacaagcc gtacaggaca
     7441 gcatccgcga catcgaccag tgctcgagcc agatctcctc ggcgcgcagc ttcgcgggca
     7501 ccacgctgca acgcctggac ggcgtcaagg gacggctcga cgcgaccgag ctttccgctc
     7561 aaacttcacg ctcgaatgcc gaagatctcg acatggtcaa ggccctgtcg agcttcaacc
     7621 agcagcagac gggctaccag gccgcgctcc agagctacgc cacgatccag aagctgtccc
     7681 tgttcaacta tctgaacgcc tgagatagcg gacgtcggac tggctcgtca ccattccaat
     7741 aaatacgttt ccgaggattc caagaatgtc cctagaccat cccgtcctcg accaggtggt
     7801 gctcggcgcg agctccctgt tcgacgccag gcgtgcgccc atcgcggccc acatgacggt
     7861 gttcccccag cggcccgacg ccccgatcga cgccgccggc ctgctgcagg tcgtcgacgt
     7921 gatcgccccg cccgaggccg ggccgctgat gctcagcgtg acgcacgagg gcagcctgcg
     7981 cgcgctgctg gccgcgccca ccgccgcgca cctgatgatc gaggtgcccg gcttcatgct
     8041 gtccgacccc ggcctggcga cgctgctgca cgagcgcgtg gcccagggcc tgtcgctggt
     8101 gctgggcggc cgcatccccg actccgcgcc gcaggacctg tggcagctgt tccaggccgt
     8161 gaccgtcgac cgcgccaaca tccgcaaccc gctgcccgcc gggctcggcg gccggccgct
     8221 gcagcgcttc ttcatcggcg ccgacggccc ggcgatgtcg gccggcgcgc tgcgcgcggg
     8281 cttttccgcc gtcgtcggct ggcccacggg cgacgccccg ggccccgaca ccaaggccaa
     8341 gaacgtgccg gccggcgtca ccaccgtcgt cgacctgatg caacgcgtgg atcgccagga
     8401 gccggccgag aagctggagg cggtggtcaa gaacgacccg acgctggcgt tccgcctgat
     8461 gcgctacctg aactcgccgg gctttggcct gtcggtcgag atctcgtcgt tccgccacgc
     8521 gctgatgatc ctgggctacc agcggctcaa gcgctggctg gcgctgctgg tcaccagtgc
     8581 catcgacgat cccgacatga agccgctgat gcagctggcg gtgcgccgcg gcctcgtgat
     8641 ggaggagatc gcgcgccccg gcggcgacga cgcgctgcgc aacgaactgt tcatctgcgg
     8701 cgtgttctcg acgctggatc gcatgatggg ccagccgttc gagcagctgc tcaagacgat
     8761 ccccgtgcag gagagcgtgg ccaaggccct ggcccacgag agcggcccgc tcgcgccgtt
     8821 cctggcgctg gcgcgcgcga tcgagaccga atcggcctac gacatccgcg aggcggccga
     8881 cacgctgatg ctgacggtgg gcgacgtgaa ccgggcgctg gccaaggcgc tggtgcacgc
     8941 tcactccctg accaagggct gagcgccagc cttcgcgccc ggtcgccagg aagggccggc
     9001 cgtgacgcgg cggcgccggc tcggaaataa ttctcggatc gacgccggcc cggcacccgg
     9061 cgccgcccgg cggcgtggag aatcgccgct ggcggatttg tcgccatgga gaggtcggga
     9121 aagatgattc ggtcgacgag gttggcagcg ggcgtcgtgc tggtggcact ggcgcagatc
     9181 ggcaacgtgc aggcgcagac ggtgcacaag tgcatcgtca acggcgccgc cgtctaccag
     9241 gccgccgcgt gcccggccgc caacgagcag aagtcgctcg tcatcccgtc ggccccgagc
     9301 cagcaggaac tgctggacgc caccgccaac gggcggctgc agtcggtcac gccgggctcc
     9361 aacgtgccgc aggtggggac gtcgcggcgc tacgaccgcc atcccgtcgt ggccagcccg
     9421 gagctgacgc cttcgatgca ggatcccgag caggtgccca ccaacagctg cgaccggctg
     9481 aaccagacct accaggatgc caagtaccgg cgcgacgagc tgtcggcacc cggcggcggc
     9541 gcgaaccgcg ccccggccct gcagcgcgcg atcgacgaca tgaagcgcgc ccaggacatg
     9601 gcggccaaca cgcagtgcca tctgcgctga gcgcgcgacc gcgggcatga ccggcacgag
     9661 accggcgccc cgccaggaac gagggaggat gcaatgatga cgaggacgta cggacgcgcc
     9721 gccagcctgg ccgccctgtt cgcgacggtg gccgtgcacg cccaggaagt gcacaagtgc
     9781 accgtcaacg ggacggtcac ctaccaggcc aggccgtgcg ccacgggcga cgtcgtgctg
     9841 ccgccggcgc ccatgcccag cgaccaggac acgcgccagg cgcaggaa
//
LOCUS       DATCRO010000584         6546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_467742, whole genome shotgun sequence.
ACCESSION   DATCRO010000584 DATCRO010000000
VERSION     DATCRO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467742"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1277
                     /locus_tag="VIN75_15985"
     CDS             87..1277
                     /locus_tag="VIN75_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEY8975720.1"
                     /translation="MSADYDSYRSALAGRRLPAAFVDLDALDANLADLRRRAGDLPIR
                     LVTKSVRCVAILRRALASSPQFQGLMCYSPAEAAWLASLGFRDLLVAYPTVEPDDLRA
                     VAAQRRAGRDITLMVDGAAQVQRIAQLAQAEGVVQPLAIDLDMSSDFPGLHFGVFRSP
                     VDGPEAALALAGEIARHPSLRLDGLMGYESQIAGLMDDVPGQAARSAVIRWLKRRSIP
                     EINERRRATVAALKAAGHALRFVNGGGTGSFESTSLDASVTELAAGSGLYVPTLFDHY
                     RAFEARPAAGFAVAVTRAPRPGIVTCAGGGYPASGPGGADRMPRPWLPAGCTLVATEG
                     AGEVQTPVRIPAGVSLAPGDPLLFRHAKAGELCERFNELLLIEGGRVVDTVPTYRGDG
                     RCFF"
     gene            1295..2581
                     /locus_tag="VIN75_15990"
     CDS             1295..2581
                     /locus_tag="VIN75_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY8975721.1"
                     /translation="MWKNWSGYVECPGTPVRTPGDVAEVARLLADAGRAGQSVRVAGA
                     GHSFSPLVASDGVIVSLDRMQGIVDVDGVTNVARVRAGTRLYALGAALAERGLAMENL
                     GDINVQSIAGATSTGTHGTGLAFGNLATQIAALKFVAADGREVVASPLDNPELFAGGR
                     VALGALGVISEVSLRLVPAFRLKLERGGMDLEDCLAQADALVAANRSFEFYWLPHTES
                     VLTKKWNITDAPVDEMGLGRWVSDVLLENTAFGALCKLGRAMPSWCPPLSRFCAAMIS
                     RSEQVDASWSMLSTVRQVRFNEMEWSLPAARGADALREIKALIARREFPLMFPLEYRW
                     VKGDDIWLSPNFGRDSVHISVHQFVGMPFERYFDAVQAICLNHGGRPHWGKVHSLKAA
                     QLSRLYPRWDDFLALRERMDPAGRFLTPYLRGLFGV"
     gene            complement(2591..3040)
                     /locus_tag="VIN75_15995"
     CDS             complement(2591..3040)
                     /locus_tag="VIN75_15995"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975722.1"
                     /translation="MPPDTDPACAAQLARAREEFDPKQARFSAEVEAYSRWELDEDTA
                     TLAFFREDGASRTYSVIPIGTWLVDRQDWAWAWADDAFPEASRRRSAVLQSFDEAGGQ
                     GRFTRPSFHAHAEDVGELCVLAVHHLGGHAQFRTMGDLRAYHVVYPR"
     gene            3220..4512
                     /locus_tag="VIN75_16000"
     CDS             3220..4512
                     /locus_tag="VIN75_16000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8975723.1"
                     /translation="MQGDRLSHGLWEATAPPAPPAPPLRGRVEVDVAVIGAGYTGLSA
                     ALHLAQAGRSVAVLEAAQVGFGASGRNVGLVNAGMWVPPEALPQRLGEVRGARLLGLL
                     GDAPSLVFDLVARHGMACEALRRGTLHCAVGASGLAGLRARCEQWQRLGAPVALLSRD
                     ETAARVGTSAYAGALLDQRAGTIQPLAYARGLARAAQAAGAALHGASPVVGAEDRGTT
                     WRLAIDGGGQVEARWVVVATNTYSLPGGPWSSIASELVRMPYFNLATRPLPADLRRTI
                     LPGGEGAWDTRTVLSSLRLDASGRLVFGSIGALRPPGHGIHLRWGRRALLRLFPALAD
                     VTFEHEWYGWIGTTADSLPRWHALARNVVSFSGYNGRGIGTGTVFGRELARLVLGEIT
                     PSDLALPERPIVPTPLRAAREVFYEAGAQVAHAVDARW"
     gene            4865..>6546
                     /locus_tag="VIN75_16005"
     CDS             4865..>6546
                     /locus_tag="VIN75_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY8975724.1"
                     /translation="MSGLIRIRGARQHNLKNLDLDIRTGELTVVTGPSGSGKSSLVFD
                     TLYAEGQRRYVETFSAYARQFLDRMDRPAVDHVEGVPPAIAIDQTNPVRSSRSTVGTM
                     TELNDHLKLLFGRAAALFDRVTALPVRHDTPDTIYADLVQRAAAQGDPRVAVTFPVEL
                     PANTTAEEVAQWLSSSGFTRIQGERTIRDDKGGERKLLDVVADRFRVGKVEKVRVVEA
                     IELALKRGQGRLNVDVLPENAEAEDPGIRWRWSTGLHCPESDLRYGDPQPALFSFNSA
                     FGACETCRGFGRVIGVDWGLVIPDEKKTLRNGAIKAIQTPAWKEIQEDLMKYAGDAGI
                     PRDTAWTQLNESQRRWVVDGSPNWNGNWNKQWYGIKRFFEYLESKAYKMHIRVLLSKY
                     RSYTTCPACDGARLKLEAQLWRVGTKAQADAVLPPSQRNLPRGTDWTREQLEALPGLG
                     LHDMMLLSIDRLRRFFDTLETPADLPDEALKLLMDEIRTRLKYLNDVGIGYLTLDRQS
                     RTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRIVEAMQRLRDA"
BASE COUNT          899 a         2284 c         2433 g          930 t
ORIGIN      
        1 gcacaaggcg atgctggtgg cgacgctcga gtcgcacagg gcgcgcggaa ggaacgcatg
       61 agcgcccgca cggcggaggt tgcgctatga gcgcggacta cgacagctac cggagcgcgc
      121 tcgccggccg ccgcctgccc gccgcgttcg tcgacctcga cgcgctggac gccaacctgg
      181 ccgacctgcg ccggcgcgcc ggcgacctgc cgatccggct ggtgaccaag tccgtgcgct
      241 gcgtcgcgat cctgcgccgc gcgctcgcgt cgtcgccgca gttccagggc ctgatgtgct
      301 actcgcccgc cgaggccgcg tggctcgcgt cgctgggctt tcgcgacctg ctggtggcct
      361 atccgacggt ggaacccgac gacctgcgcg ccgtcgcggc gcagcggcgc gcgggccggg
      421 acatcacgct gatggtggac ggcgcggcgc aggtgcagcg catcgcgcag ctcgcgcagg
      481 ccgagggcgt ggtgcagccg ctggcgatcg acctggacat gtcgtccgac ttccccggcc
      541 tgcacttcgg cgtgttccgt tcgccggtcg acggtcccga agccgcgctc gcgctggccg
      601 gcgagatcgc acgccatccg tcgctgcggc tggacggcct gatgggctac gagagccaga
      661 tcgcggggct gatggacgac gtccccggcc aggcggcgag gagcgcggtg atccgctggc
      721 tcaagcgccg ttcgatcccc gagatcaacg agcgccgccg cgcgacggtc gccgccctga
      781 aggccgcggg ccacgcgctg cgcttcgtca acggcggcgg caccggcagc ttcgagagca
      841 cgagcctgga tgcgagcgtc accgagctgg cagccggctc cggcctgtac gtgcccacgc
      901 tgttcgacca ctaccgcgcg ttcgaggcgc gtcccgccgc gggcttcgcg gtggcggtca
      961 cgcgggcgcc caggccgggc atcgtcacct gcgcgggcgg cggctatccg gcctcgggac
     1021 cgggcggcgc cgaccgcatg ccgcggccct ggctgcccgc gggctgcacg ctggtcgcca
     1081 cggagggcgc gggcgaggtg cagacgccgg tgcgcatccc ggccggggtg tcgctggcgc
     1141 cgggcgatcc gctgctgttc cgccacgcca aggcgggcga gctgtgcgag cgcttcaacg
     1201 agctgctgct gatcgaaggc ggtcgcgtgg tcgatacggt gcccacgtat cgcggcgacg
     1261 gccgctgctt cttctaggaa cgagggtcga cgggatgtgg aagaactggt cggggtatgt
     1321 cgagtgtccg ggcacgccgg tgcgcacgcc cggcgacgtc gccgaagtgg cgcggctgct
     1381 ggccgacgcc gggcgcgcag ggcagtcggt gcgcgtggct ggcgcgggcc actcgttcag
     1441 cccgctggtg gccagcgacg gcgtcatcgt ctcgctggac cggatgcagg gcatcgtcga
     1501 cgtcgacggc gtcacgaacg tcgcgcgcgt gcgggccggc acgcgcctgt acgcgctcgg
     1561 cgccgcgctg gccgaacgcg ggctcgcgat ggagaacctg ggcgacatca acgtgcagtc
     1621 catcgccggc gccaccagca ccggcacgca cggcacgggc ctggccttcg gcaacctcgc
     1681 cacgcagatc gcggcgctga agttcgtcgc cgccgacggc cgcgaggtcg tcgcgtcgcc
     1741 cctggacaac cccgagctgt tcgcgggcgg ccgcgtcgcg ctcggcgcgc tgggcgtgat
     1801 cagcgaggtc agcctgcgcc tggtgcccgc gttccggctc aagctggagc gcggcgggat
     1861 ggacctggag gactgcctcg cgcaggccga cgcgctcgtc gcggcgaacc ggtcgttcga
     1921 gttctactgg ctgccgcaca ccgagtcggt cctgaccaag aagtggaaca tcaccgacgc
     1981 gcccgtcgac gagatgggcc tggggcgctg ggtgagcgac gtgctgctgg agaacacggc
     2041 gttcggcgcg ctgtgcaagc tgggccgggc gatgccgtcg tggtgcccgc cgctgagccg
     2101 cttctgcgcg gccatgatct cgcgcagcga gcaggtcgac gcgagctggt cgatgctgtc
     2161 gacggtgcgc caagtgcgct tcaacgagat ggagtggtcg ctgcccgccg cgcgcggggc
     2221 cgacgcattg cgcgagatca aggcgctgat cgcgcgccgc gagttcccgc tgatgttccc
     2281 gctcgagtac cgctgggtca agggcgacga catctggctg agcccgaact tcggccgcga
     2341 cagcgtgcac atctcggtgc accagttcgt gggcatgccc ttcgagcgct acttcgacgc
     2401 ggtgcaggcc atctgcctga accacggcgg ccggccgcac tggggcaagg tgcattcgct
     2461 caaggcggcg caactgtcgc ggctgtatcc gcgctgggat gatttcctcg cgctgcgcga
     2521 gaggatggat ccggcaggcc gcttcctgac gccgtacctg cgcgggttgt tcggcgtgtg
     2581 aggccggcac tcagcgcggg tagacgacgt ggtaggcgcg caggtcgccc atcgtccgga
     2641 actgcgcgtg gccgcccagg tggtggaccg ccagcacgca cagctcgccg acgtcctccg
     2701 cgtgggcatg gaacgagggg cgcgtgaagc ggccctggcc accggcctcg tcgaacgact
     2761 gcaggaccgc ggaacggcgg cgggacgcct cggggaacgc atcgtcggcc caggcccatg
     2821 cccagtcctg gcggtcgacc agccaggtgc cgatcgggat cacggaatac gtgcgcgacg
     2881 caccgtcctc gcggaagaac gccagcgtgg cggtgtcctc gtcgagctcc cagcgcgaat
     2941 acgcctcgac ctccgccgag aagcgcgcct gcttgggatc gaactcctcc cgcgcgcgtg
     3001 caagctgggc ggcgcaggcg ggatcggtgt cgggcggcat gggcgttcgg cgggaatcgg
     3061 agtgcggcca gtatgcagga agcgtcctcg gcaggtccgc gtgggcggcg ctgcgatctg
     3121 cgggcgcgaa cgatcagccg gattggacgt gccgcaagca tcgaggagac gtcccgtccg
     3181 ggtggcgccg agcgtggccg ggtgcgtcag aatggccgca tgcaagggga tcgtctgtcg
     3241 cacggcctgt gggaggccac cgcgccgccc gccccgccgg cgccaccgct gcgcggccgc
     3301 gtggaggtcg atgtcgccgt gatcggcgcc ggctacaccg ggctgtcggc ggcgctgcac
     3361 ctggcccagg ccggacgatc ggtggccgtg ctggaggcgg cgcaggtcgg gttcggggcg
     3421 tcggggcgca atgtcgggtt ggtcaacgcc ggcatgtggg tgccgcccga ggcactgccg
     3481 caacgcctcg gcgaggtccg cggtgcgcgc ctgctggggc tgctgggcga tgcgccgtcg
     3541 ctggtgttcg atctcgtcgc gcggcatggc atggcgtgcg aggcgctgcg tcgcggcacg
     3601 ctgcattgcg cggtgggtgc gtcgggcctc gcgggcctgc gcgcgcgctg cgagcaatgg
     3661 cagcggctgg gtgcgccggt ggcgctgctg tcgcgcgacg agactgctgc gcgcgtcggc
     3721 acgagcgcct acgccggcgc gttgctcgat caacgcgccg gcacgatcca acccctcgcg
     3781 tacgcgcgcg ggctggcgcg cgccgcgcag gctgcgggtg cggcgctgca tggcgccagt
     3841 ccggtcgtcg gggccgagga tcgcgggacg acgtggcggc tggcgatcga cggcggcggc
     3901 caggtcgagg cgcgctgggt ggtggtcgcg accaatacgt attcgctccc tggcggcccc
     3961 tggtcgtcga tcgcctccga gctggtgcgc atgccgtact tcaacctcgc cacgcgcccg
     4021 ttgcccgccg acctgcggcg caccatcctg ccgggcggcg agggcgcgtg ggacacccgc
     4081 accgtcctca gctcgctgcg cctggacgcg tcgggccggc tcgtgttcgg cagcatcggc
     4141 gcgctgcgcc cgccgggcca tggcatccac ctgcgctggg ggcggcgcgc gctgctgcgc
     4201 ctgttccccg cgctcgcgga cgtgaccttc gagcacgagt ggtacggctg gataggcacg
     4261 accgccgatt cgctgccacg ctggcacgcg ctggcgcgca acgtggtcag cttcagcggc
     4321 tacaacgggc gcggcatcgg caccggcacc gtgttcggcc gcgagttggc gcgcctggtg
     4381 ctgggcgaga tcaccccatc cgacctggcc ctgccggagc gacccatcgt tccgacgccg
     4441 ctgcgcgcgg cgcgcgaggt gttctacgag gcgggggcgc aggtggcgca cgctgtcgac
     4501 gcgcggtggt gaccggtgcg tcctgcgacc gcgggccgag ccgacatcct gcgcgcggtc
     4561 gcgggatccc tctcggccgg ccaggcaccg gtttcgccgc gcgatgcggc tgtccaggcc
     4621 tggatgccgg aatagagttt gactccgctc cgcgcaggat gacggcccgg ccggcggcga
     4681 gggacggctc ggccggcggc aagagctgcg gcgatcgtca gtcatgccgg tgtcccagca
     4741 tcgccagatg aggccagcgg cgtccaattc aagaacatcc gacatcgcgc agggccattg
     4801 actgctgaca agcgctggca gttggcgccc ctagaatctt ccgcctaccc ctggaaggac
     4861 gcacatgagc gggctcatcc gcatccgcgg cgcgcggcag cacaacctga agaacctcga
     4921 cctggacatc cgcacgggcg agctgacggt cgtcacgggg cccagcgggt cgggcaagtc
     4981 cagcctcgtg ttcgacacgc tgtacgcgga aggccagcgg cgctacgtcg agacgttctc
     5041 tgcctacgcg cgccagttcc tggaccgcat ggaccgtccc gcggtggatc acgtcgaagg
     5101 cgtgccgccc gcgatcgcca tcgaccagac caacccggtg cgcagctcgc gctccacggt
     5161 cgggacgatg actgagctga acgaccacct caagctgctg ttcggccgcg ccgcggcgct
     5221 gttcgaccgc gtcaccgcgc tgccggtgcg ccacgacacg cccgacacga tctacgccga
     5281 cctggtgcag cgcgccgccg cgcagggcga tccgcgcgtg gcggtcacgt tcccggtcga
     5341 gctgccggcc aacaccacgg ccgaggaagt ggcgcagtgg ctgtcctcca gcggcttcac
     5401 gcgcatccag ggcgagcgca cgatcaggga cgacaagggc ggcgagcgca agctgctcga
     5461 cgtcgtggcc gaccgcttcc gcgtcggcaa ggtcgagaag gtgcgcgtgg tcgaggccat
     5521 cgagctggcg ctcaagcgcg gccaggggcg gctgaacgtc gacgtgctgc cggagaacgc
     5581 cgaggccgag gatcccggca tccgctggcg ttggtcgacg ggcctgcact gccccgagag
     5641 cgacctgcgc tacggcgacc cgcagccggc gctgttctcg ttcaactccg cgttcggcgc
     5701 ctgcgagacc tgccgcggct tcggccgcgt gatcggcgtc gactggggcc tggtcatccc
     5761 cgacgagaag aagacgctgc gcaacggcgc gatcaaggcg atccagacgc ctgcctggaa
     5821 ggagatccag gaagacctga tgaagtacgc gggcgacgcc ggcatccccc gcgacaccgc
     5881 gtggacccag ctgaacgagt cgcagcgccg ctgggtggtc gacggttcgc cgaactggaa
     5941 cggcaactgg aacaagcagt ggtacggcat caagcgcttc ttcgagtacc tcgagtcgaa
     6001 ggcgtacaag atgcacatcc gcgtgctgct gtccaagtac cgcagctaca cgacgtgccc
     6061 ggcgtgcgac ggggcgcgcc tgaagctgga agcgcagctg tggcgcgtgg gcacgaaggc
     6121 gcaggccgac gcggtgctgc cgccgtcgca gcgcaacctg ccgcgcggca ccgactggac
     6181 gcgcgagcag ctcgaggcgc tgccgggcct gggcctgcac gacatgatgc tgctgtcgat
     6241 cgaccgtctc cgacgcttct tcgacacgct ggagacgccc gccgatctcc ccgatgaagc
     6301 gctcaagctg ctgatggacg agatccgcac gcggctgaag tacctcaacg acgtcggcat
     6361 cggctacctc acgctcgatc gccagagccg cacgctctcg ggtggcgagg tgcagcgcat
     6421 caacctcacc accgcgctgg gcacctcgct ggtcaacacg ctgttcgtgc tggacgagcc
     6481 gtccatcggc ctgcacccgc gcgacatgca ccgcatcgtc gaggcgatgc agcgcctgcg
     6541 cgacgc
//
LOCUS       DATCRO010000585         8300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_73803, whole genome shotgun sequence.
ACCESSION   DATCRO010000585 DATCRO010000000
VERSION     DATCRO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73803"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VIN75_16010"
     CDS             complement(<1..1431)
                     /locus_tag="VIN75_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HEY8975725.1"
                     /translation="MNAPSQVEPLALRAADLLQRIEAYGFDEPDEPLAFESRLADDQG
                     WTLGYALAVVEEYRRFLVLTQVAGHAVSPSPDVDEAWHLHLTRTAHYEAFCAAVFGRF
                     LHHAPARSGEGAKHRDMYAATLEAYRAAFGFTAPPVIWPRPGQPLARQAATVPGWTVP
                     ESLRRDRRLGLAALLLAVVAGVSLDRVGLLHPLQGIGPVQFLAGALIVTLALGWLGLR
                     SGVPPVQATARDRLEPYEAAWFSGGAARMAMTAIAALTERGVLLAPGKAPERGARPLI
                     PVNRTVQAHCVHPAEAVCLAMATDAGLRYTDACNALQPLAAEVEQRLVAAGIAADTAA
                     LPRPRAQALLGMEILLVVEVARIAHALGTPHPVGFLVLLTLAGMGLAWALARRPLRTS
                     ARSERVLRPLRLAAGIPGKQPRAGEALAFGVALIGGTAIAGDLRFAGLDQQVDVSGAD
                     WALRQRRGAGRDGSSDGSNSCGSSCGS"
     gene            complement(1507..2832)
                     /locus_tag="VIN75_16015"
     CDS             complement(1507..2832)
                     /locus_tag="VIN75_16015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HEY8975726.1"
                     /translation="MLTQVAGTQVCPSDDVDQAWHLHITRTADYERFCRDVFGRFLHH
                     RPAEAGPDEHQRHRGMYVGTLSLYRRAFRQPAPADVWPDVDRRFARPLAGSDAIRLPG
                     ELASGALLTIVGLLGSFALALLLNRLGLLDASHEVSGPRFLQFAVPATMLLVLLGWLS
                     TSPFDRGLPRDTLDAYEAAWLSGGTPRVAATAIGMLVELGRLELRTTVEGVGWRRRSV
                     TRLVVADAAPSDLLHPVENACLHSARDGVVRFERADVALRSWAMRTGARLRRAGLAGD
                     GSAIAPARAAVAIVTAGWLAVELERIGHALGSARPMSYLVLLAIVTATSFVLLALRDG
                     GQPTWRGRRALRALRETLRGENARAAERARRQGTEPVIAARLLPMTLALLGPAAVLAR
                     PGFDGLDDAIGPDGMRHAGGNSGSSGDGGGGGCSGGGGCGGGCGGGCGG"
     gene            complement(3171..4505)
                     /locus_tag="VIN75_16020"
     CDS             complement(3171..4505)
                     /locus_tag="VIN75_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8975727.1"
                     /translation="MDKHGSPIIKINDVRKSFRAADGRERPVLEGVDFTLREGEIVAL
                     LGQSGSGKSTLLRIMAGLIGVDSGSVQYRGQPLYGPARAISMVFQSFALFPWLTVQQN
                     VELGLEARGIGREERAERAEKAISLIGLAGFEGALPRELSGGMRQRVGIARALVTQPD
                     VLLMDEAFSALDVLTGERLRDDILELWTQGGMPTKAMLVVSHNIEEAVLMADRILIFA
                     SDPGRVRFQLAIDLPRPRVPDSAGVRALIDEVYALMTAGAVRAGRAAEEPEEMRLHDR
                     LPDADVARMEGLLELLAGAPFHGEADLPKLAEESELTDDELLPVASAIDLLGLARVQS
                     GDLHLTLLGRRYVDGDHAVRQELFGQQVLANVPLAAHIRHSLEQDPERALPEQPFLRL
                     LQQQLDPAEAERVLKTVIEWGRHGEVFEYDFHAGSIHLPDDQQDEARNSIIE"
     gene            complement(4549..6276)
                     /locus_tag="VIN75_16025"
     CDS             complement(4549..6276)
                     /locus_tag="VIN75_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8975728.1"
                     /translation="MAFEKNFFPRQVVEAGNRWDWALLPLVLAALVLAGLGATGMTRP
                     YHLGDPIPLSLDPLQLPYYLLRTTLRMLLALVFSLLFSFAFATAAARWRAAEKVLVPL
                     LDILQSIPILGFLSITVTGFIALFPGNLFGVECAAIFAIFTSQAWNMAFSLYQSMRTV
                     PGELQEAARVFQLSPWQRFWRLELPFAMPGLLWNMMVSMSGGWFFVVASEAIAVSGQD
                     IKLPGVGSYIAMAIEARNLQAIGWAILGMLIGIVLYDQLLFRPLVAWADKFRFEEGGQ
                     EAAPTSWLLTWSRRTERIAKLSERFTHALESTFTWFRVKHDGTSIRARATPHSGTIAR
                     VWDALIAAGGLYAAWELYSFVHAEVGFAEVWHVFLLGCATMLRVVVLIALASLVWVPI
                     GVWIGLRPAVSGRLQAVAQFLAAFPANLMFPVVVVFIVHWHLNPNIWLSPLMVFGTQW
                     YLLFNVIAGASSIPSELRFAAGNLGLTGWLRWKRFFLPAVFPSFVTGAITASGGSWNA
                     SIVSEYVSWGDTRLEAQGLGSYIAHWTAHGDFHRIALGICVMCVFVMGMNHFVWRRLY
                     RIAEERMHF"
     gene            complement(6392..>8300)
                     /gene="secA"
                     /locus_tag="VIN75_16030"
     CDS             complement(6392..>8300)
                     /gene="secA"
                     /locus_tag="VIN75_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8975729.1"
                     /translation="RAEELLAQAGLLVEGASLYDPANITLMHHVYAALRANHLYHRDQ
                     HYVVQNGEVVIVDEFTGRLMTGRRWSDGLHQAVEAKEGVEIQSENQTLASITFQNYFR
                     MYTKLGGMTGTADTEAYEFQEIYGLETVVIPPNRPTQRKDELDLVYKTEREKYNAVVE
                     DIRDCVNRGQPALVGTTSIENSEKLSQLLEKARIPHQVLNAKQHEREALIVAQAGRPG
                     TITIATNMAGRGTDIVLGGNVEKQIEHIEADDAIPADQKAAQIQKLKDEWQALNEQVK
                     AAGGLRIIATERHESRRIDNQLRGRSGRQGDPGASRFYLSLEDPLMRIFAGDRVKAIM
                     ERLKMPEGEAIEAGIVSRSLEGAQRKVEARNFDMRKQLLEYDDVANDQRKVIYQQRNE
                     ILEAKSLVNQIANLREGTMEDLVRAFVPAESVEEQWDLPGLQKALRDEYLLDVPIAEQ
                     VEKSESVNDEEVVQMVVTAAKAAFQAKLDLVGEEQFTPFMRMVLLQHLDTNWREHLAA
                     LDYLRQGIHLRGYAQKNPKQEYKREAFELFSQLIDTVKTDVTKVLLTVRVQTQEQAAQ
                     AAQAIEQRAEHVSNVQYVHPNEDGSISQDAAPEGLSAANPNLPDRRVGRNDPCPCGSG
                     KKFKECHGKLA"
BASE COUNT         1197 a         2958 c         2940 g         1205 t
ORIGIN      
        1 gctgccgcag gacgagccgc acgagttcga gccgtccgac gagccgtccc tgccggcgcc
       61 cctgcgctgg cgcagcgccc agtcggcgcc cgacacgtcg acctgctggt cgaggccggc
      121 gaagcgcagg tcgccggcga tcgccgtgcc gccgatcagc gcgacgccga acgcgagcgc
      181 ctcgcccgcg cgcggctgct tgccggggat gcccgccgcc agccgcagcg gccgcagcac
      241 gcgctccgaa cgcgcgctgg tgcgcagcgg gcggcgcgcc agcgcccagg ccagccccat
      301 gccggccagc gtcagcagca ccaggaagcc gaccgggtgc ggcgtgccca gggcatgcgc
      361 gatgcgcgcg acttccacca ccagcaggat ctccatgccc agcagcgcct gcgcgcgcgg
      421 ccgcgggagc gcggcggtgt cggccgcgat gccggcggcg accaggcgct gctcgacctc
      481 ggcggccagc ggctgcaacg cgttgcaggc atcggtatag cgcaggccgg cgtcggtggc
      541 catcgccagg cagacggcct cggccggatg gacgcagtgc gcctggaccg tgcggttgac
      601 cgggatgagc ggccgcgcgc cgcgctcggg cgccttgccg ggcgccagca gcacgccgcg
      661 ttcggtgagc gccgcgatcg ccgtcatcgc catgcgggcc gcgccgccgc tgaaccatgc
      721 cgcctcgtag ggctccagcc ggtcgcgcgc cgtcgcctgc accggcggca cgcccgagcg
      781 cagtcccagc cagcccagcg ccagcgtgac gatcaaggct cccgcgagga actggaccgg
      841 cccgatccct tgcagcggat gcagcaggcc cacgcggtcg agcgacacgc cggccacgac
      901 cgccagcagc agcgcggcga ggcccagtcg gcggtcgcgc cgcagcgact ccggcaccgt
      961 ccagcccggc accgtggccg cctggcgcgc cagcggctgg ccaggccgtg gccagatcac
     1021 cggcggcgcg gtgaacccga aggcggcgcg gtaggcctcc agcgtcgcgg cgtacatgtc
     1081 gcggtgcttc gcaccctcgc ccgaacgcgc cggcgcgtgg tgcaggaagc gcccgaacac
     1141 ggcggcgcag aacgcctcgt agtgcgccgt gcgcgtcagg tgcaggtgcc aggcctcgtc
     1201 gacgtcgggc gacgggctca ccgcgtggcc cgcgacctgt gtcagcacga ggaagcggcg
     1261 gtactcctcg acgaccgcga gcgcgtagcc gagcgtccag ccctggtcgt cggccaggcg
     1321 cgactcgaag gcgagcggct cgtcgggctc gtcgaagccg tatgcctcga tgcgttgcag
     1381 caggtcggcg gcgcgcagcg ccaggggttc gacttgcgaa ggtgcgttca tgcgcgggtc
     1441 actccttggt cgagcgggtt atagcgtcgt cgcgcgtgct gcgcgtcgcc atcaaggggg
     1501 ttgccgtcat ccgccgcatc ccccaccgca cccgccaccg catccgccgc cgccactgca
     1561 tccaccgccg ccgccgtcgc ccgacgaacc gctgttcccg ccggcgtggc gcatgccgtc
     1621 cggaccgatc gcgtcgtcca ggccgtcgaa gccgggtcgg gccagcacgg cggccggccc
     1681 gagcagcgcg agcgtcatgg gcagcagtcg cgcggcgatg accggctcgg tgccctgccg
     1741 cctggcgcgt tccgcggccc gcgcgttctc gccgcgcagg gtttcccgga gcgcacgcaa
     1801 cgcacgccgg ccccgccagg tgggctggcc gccatcgcgc agggccagca ggacgaagct
     1861 cgtcgccgtg acgatggcca gcagcaccag gtacgacatc ggccgcgcgc tcccgagcgc
     1921 gtgcccgatg cgttcgagct ccacggccag ccagccggcc gtcacgatgg ccacggcggc
     1981 gcgggccggc gcgatggccg agccgtcgcc ggccagcccg gcccgccgca gccgcgcgcc
     2041 ggtccgcatc gcccaggagc gcagcgcgac gtccgcgcgt tcgaagcgca cgacgccgtc
     2101 gcgcgccgag tgcaggcagg cgttctccac cgggtgcagc aggtccgacg gcgccgcgtc
     2161 ggccaccacg agccgcgtga cggaacggcg ccgccagccg accccctcga ccgtcgtgcg
     2221 cagttcgagt cgaccgagct cgaccagcat gccgatcgcg gtggccgcca cgcgcggcgt
     2281 gccgcccgag agccacgccg cctcgtaggc gtccagcgtg tcgcgcggca ggccgcggtc
     2341 gaagggcgag gtcgacagcc atccgagcag caccaggagc atcgtcgccg gcaccgcgaa
     2401 ctgcaggaat cgcggcccgc tcacctcgtg cgaggcgtcc agcaggccca gccggttcag
     2461 cagcagtgcc agcgcgaacg agccgagcag tcccacgatc gtgagcagcg cgccggacgc
     2521 gagctcgccg ggaaggcgga tcgcgtcgga cccggccagg ggtcgcgcga accgccggtc
     2581 gacgtccggc cacacgtccg ccggcgcagg ctgccggaac gcgcgccgat acagcgacag
     2641 cgtgcccacg tacatgccgc gatgccgctg gtgttcgtcg ggccccgctt cggccggcct
     2701 gtgatgcagg aagcgtccga acacgtcgcg gcagaagcgc tcgtagtcgg cggtgcgggt
     2761 gatgtggagg tgccaggcct ggtcgacgtc gtccgacgga cagacctgcg tgccggcgac
     2821 ctgcgtgagc acgaggaagc ggcggtattc gcggacgacg cggacggcgt gtccgagggt
     2881 ccagccgttg tcgtcggcca gccggctctc gaaggcgagc ggatcgcccg gagcggaaag
     2941 ggggtgggcc tcgacgcgcg ccagcagcgt ggcggcggag gtggggagct cgttgtgcga
     3001 gtgactggtg atgggaacct tcctttcttg ttgttatggt ctcttgttgt tgttgcgcgc
     3061 agtgtcaagt cggcccgccg ggccggcaat cgtgggaaac cacatgcgcg aattcggcga
     3121 atggcggcgc ttcgcggaga tgacggctgc gcactcccgc gctggcgcgc ctactcgatg
     3181 atggagttgc gcgcctcgtc ctgctggtcg tcgggcaggt ggatgctgcc ggcgtggaag
     3241 tcgtactcga agacctcgcc gtggcggccc cattcgatga ccgtcttgag cacgcgttcg
     3301 gcctcggccg gatcgagctg ctgctgcagc aggcgcagga agggctgctc gggcagcgcg
     3361 cgctcgggat cctgctcgag gctgtggcgg atgtgcgcgg ccagcggcac gttggcgagc
     3421 acctgctggc cgaacagctc ctggcgcacg gcatggtcgc cgtcgacgta gcgccggccc
     3481 agcagcgtca ggtgcaggtc gccgctctgg acgcgcgcca ggcccagcag gtcgatcgcg
     3541 ctcgcgacgg gcagcagctc gtcgtcggtg agctccgatt cctcggccag cttgggcagg
     3601 tcggcctcgc cgtggaacgg ggccccggcc agcagctcca gcaggccctc catgcgcgcg
     3661 acgtcggcgt cgggcaggcg gtcgtgcagg cgcatctcct cgggttcctc cgcggcgcgg
     3721 ccggcgcgca cggcgccggc ggtcatcagc gcgtagacct cgtcgatcag cgcgcgcacg
     3781 ccggcggagt cgggcacgcg cgggcgcggc aggtcgatgg cgagctggaa gcgcacgcgg
     3841 cccgggtcgc tcgcgaagat caggatgcgg tcggccatca gcaccgcctc ctcgatgttg
     3901 tgcgacacca cgagcatcgc cttggtgggc atgccgccct gcgtccacag ctcgaggatg
     3961 tcgtcgcgca ggcgttcgcc ggtgagcacg tccaacgccg agaacgcctc gtccatcagc
     4021 agcacgtccg gttgcgtgac gagcgcgcgc gcgatgccca cgcgctggcg catgccgccc
     4081 gagagctcgc gcggcagcgc gccttcgaag ccggccaggc cgatcaggct gatcgccttc
     4141 tcggcgcgct cggcgcgctc ctcgcgtccg atcccgcgcg cctccaggcc cagctcgacg
     4201 ttctgctgca ccgtcagcca cgggaacagc gcgaaggact ggaacaccat gctgatcgcg
     4261 cgcgccggcc cgtacagcgg ctggccacgg tactgcacgc tgcccgagtc cacgccgatc
     4321 aggccggcca tgatgcgcag cagcgtgctc ttgcccgagc ccgactggcc cagcagcgcc
     4381 acgatctcgc cctcgcgcag cgtgaagtcc acgccttcga gcaccggccg ctcgcggccg
     4441 tccgccgcgc ggaaggactt gcggacgtcg ttgatcttga tgatggggga gccgtgcttg
     4501 tccatgtcgt tcgaatcttt gatcaggtgg ctgcgccttg caacggccct agaagtgcat
     4561 gcgctcttcg gcgatgcgat agaggcggcg ccacacgaag tggttcatgc ccatcacgaa
     4621 cacgcacatc acgcagatgc ccagcgcgat gcggtggaag tcgccgtgag ccgtccagtg
     4681 cgcgatgtag ctgcccaggc cctgcgcctc gagccgggtg tcgccccagc tgacgtactc
     4741 cgacacgatg ctcgcgttcc atgagccgcc gctggccgtg atggcgccgg tgacgaagct
     4801 cgggaacacc gccggcagga agaagcgctt ccagcgcagc cagccggtca gcccgaggtt
     4861 gccggcggcg aagcgcagct ccgacgggat cgacgacgcg ccggcgatca cgttgaacag
     4921 caggtaccac tgcgtgccga acaccatcag cggcgacagc cagatgttcg ggttcaggtg
     4981 ccagtgcacg atgaacacga cgaccaccgg gaacatgaga ttggccggga acgccgccag
     5041 gaactgcgcc accgcctgca gccggcccga gaccgccggc cgcagcccga tccacacgcc
     5101 gatgggcacc cacacgagcg acgccagcgc gatcagcacc accacccgca gcatcgtcgc
     5161 gcagcccagc aggaacacgt gccagacctc ggcgaagccg acctcggcgt gcacgaagct
     5221 gtacagctcc caggcggcat agaggccgcc ggccgcgatc agtgcgtccc acacccgcgc
     5281 gatcgtgccg ctgtgcggcg tggcccgcgc gcggatcgac gtgccgtcgt gcttcacgcg
     5341 gaaccacgtg aacgtgctct ccagcgcatg cgtgaaacgc tcgctgagct tggcgatgcg
     5401 ttcggtgcgg cgcgaccagg tcagcagcca agacgtcggc gccgcctcct ggccgccctc
     5461 ctcgaagcgg aacttgtcgg cccacgccac cagcgggcgg aacagcagct ggtcgtacag
     5521 cacgatgccg atgagcatgc ccaggatggc ccagccgatc gcctgcaggt tgcgcgcctc
     5581 gatcgccatc gcgatgtacg agcccacgcc cggcagcttg atgtcctggc ccgacacggc
     5641 gatggcctcg gacgcgacga cgaagaacca gccgcccgac atcgacacca tcatgttcca
     5701 cagcagcccg ggcatcgcga acggcagctc caggcgccag aagcgctgcc acggcgacag
     5761 ctggaacacg cgcgccgcct cctgcagctc gcccggcacc gtgcgcatcg actggtacag
     5821 gctgaacgcc atgttccagg cctgcgacgt gaagatcgcg aagatggccg cgcactcgac
     5881 gccgaacagg ttgcccggga acagcgcgat gaagccggtg acggtgatcg acaggaagcc
     5941 caggatcggg atcgactgca ggatgtccag cagcggcacc agcaccttct cggcggcgcg
     6001 ccagcgcgcg gccgcggtgg cgaacgcgaa gctgaacagc agcgagaaca cgagcgcgag
     6061 cagcatgcgc agcgtcgtgc gcagcaggta gtacggcagc tgcagcggat cgagcgacag
     6121 cgggatcggg tcgcccaggt ggtagggccg cgtcatcccg gtggccccca ggccggccag
     6181 caccagcgcg gccagcacca gcggcagcag cgcccagtcc cagcggttgc cggcctcgac
     6241 gacctggcgg gggaagaagt tcttctcgaa agccatgaag gcggattgtc gcggctaaag
     6301 cttgctcagg aaggacaaag ccgcatccgg ctgtgcgccg atgcggcttc gttggattcc
     6361 ggcccgcgtt cagcggaccg tcacggcaat gtcacgccag tttgccgtgg cattccttga
     6421 acttcttgcc actgccgcac gggcacgggt cgttgcggcc aacgcggcgg tcgggcaggt
     6481 tcgggttggc ggcggacagg ccttcgggcg cggcgtcctg gctgatgctg ccgtcctcgt
     6541 tcgggtgcac gtactgcacg ttgctgacgt gctctgcacg ctgctcgatc gcctgcgccg
     6601 cctgggcggc ctgttcctgc gtctgcacgc gcaccgtcag cagcaccttg gtcacgtcgg
     6661 tcttgaccgt gtcgatcagc tgcgagaaca gctcgaaggc ctcgcgcttg tattcctgct
     6721 tcgggttctt ctgcgcatag ccgcgcaggt ggatgccctg gcgcaggtag tccagcgcgg
     6781 ccaggtgctc gcgccagttg gtgtccaggt gctgcagcag caccatgcgc atgaacggcg
     6841 tgaactgctc ctcgccgacg aggtcgagct tggcctggaa cgcggccttg gcggccgtga
     6901 cgaccatctg gacgacctcc tcgtcgttga ccgactcgga tttctcgacc tgttcggcga
     6961 tgggcacgtc cagcaggtac tcgtcgcgca gcgccttctg caggcccggc aggtcccact
     7021 gctcctcgac gctctcggcc ggcacgaagg cccgcacgag gtcctccatg gtgccttcgc
     7081 gcaggttggc gatctggttg accagcgact tggcctccag gatctcgttg cgctgctggt
     7141 agatcacctt gcgctggtcg ttggcgacgt cgtcgtattc gagcagctgc ttgcgcatgt
     7201 cgaagttgcg cgcctcgacc ttgcgctgcg cgccttcgag gctgcggctg acgatgcccg
     7261 cctcgatggc ctcgccctcg ggcatcttca ggcgctccat gatcgccttg acgcggtcgc
     7321 ccgcgaagat gcgcatcagc gggtcttcca gcgacaggta gaagcgcgag gcgcccgggt
     7381 cgccctggcg gccggagcgg ccgcgcagct ggttgtcgat gcggcgcgac tcgtggcgct
     7441 cggtggcgat gatgcgcagg ccgccggcgg ccttgacctg ctcgttgagc gcctgccact
     7501 cgtccttgag cttctggatc tgcgcggcct tctggtcggc cgggatcgca tcgtcggcct
     7561 cgatgtgctc gatctgcttc tcgacgttgc cgcccagcac gatgtcggtg ccgcggccgg
     7621 ccatgttggt ggcgatggtg atcgtgcccg ggcggcccgc ctgcgcgacg atcagcgcct
     7681 cgcgctcgtg ctgcttggcg ttgagcacct ggtgcgggat gcgcgccttc tcgagcagct
     7741 gcgacagctt ctcggagttc tcgatggacg tggtgcccac cagcgccggc tggccgcggt
     7801 tgacgcagtc gcggatgtcc tcgacgaccg cgttgtactt ctcgcgctcg gtcttgtaga
     7861 cgaggtcgag ctcgtccttg cgctgcgtcg ggcggttcgg cgggatcacc acggtctcca
     7921 ggccgtagat ctcctggaac tcgtaggcct cggtgtcggc cgtgccggtc atgccaccga
     7981 gcttggtgta catgcggaag tagttctgga aggtgatcga ggcgagcgtc tggttctcgc
     8041 tctggatctc cacgccttcc ttggcctcga cggcctggtg caggccgtcc gaccagcggc
     8101 ggccggtcat caggcggccc gtgaattcgt cgacgatcac cacctcgccg ttctgcacga
     8161 cgtagtgctg gtcgcggtgg tacaggtggt tcgcgcgcag cgccgcgtac acgtggtgca
     8221 tcagcgtgat gttggccggg tcgtacaggc tcgcgccctc gaccagcagg ccggcctgcg
     8281 ccagcagttc ctcggcgcgc
//
LOCUS       DATCRO010000586         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_772069, whole genome shotgun sequence.
ACCESSION   DATCRO010000586 DATCRO010000000
VERSION     DATCRO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772069"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1365)
                     /locus_tag="VIN75_16035"
     CDS             complement(277..1365)
                     /locus_tag="VIN75_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8975730.1"
                     /translation="MSSPYHVDPDITVASTLPSAFYRDPAAYERVLERVFARSWQWIG
                     DTADVAAPGALSPREMLPGSLAEPLLLARDGAGTLRCLSNVCTHRGNLLVKAPCVAEQ
                     IRCGYHSRRFDLAGRMTFMPEFGAARNFPSPSDHLPHVPFGTLGNQAFAAVRPAAPLD
                     DFLGDLRTRLAWLPLHEFTHDPARDRNFDIAAHWALYVENYLEGLHIPFIHPALNKVL
                     SFEGYDYQLQRHSNLQFARARDGEQAFELPPSSPDFGERVAAYYYWIFPNLMLNFYPW
                     GLSVNVVQPRGMDRTRVIFRSYVWDAARLGDGAGNDLDQVEMEDEEVVQQVQRGVQSR
                     FYDRGRYSPSKERGVHQFHRLLCEFLAR"
     gene            1445..2638
                     /locus_tag="VIN75_16040"
     CDS             1445..2638
                     /locus_tag="VIN75_16040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8975731.1"
                     /translation="MTRRSSAFNARLVFTGVALASGGIAFYVPLMLSAVGLAHSGAWA
                     AGILFGTNLGRLVGSHLASRHGVFTQRRGAIVGNILLEGVALFSMAFIASPAGLVAVA
                     TLAGLGSGLSFPGMKSCLMRLPGVDSARVFAGLSLALRLGMALGYLAGALVGANHLTA
                     VFAVLLVMFVGYAGLMDLAMRDIASAPAEPSATPAPQAAPAPSADAPLPVGLTPVLLA
                     NAVFWFLTIQPTVTMSLYVPRYVPGLAVSTTYWVTTVTVLLLQLRVTRLARGMPGHLA
                     FLRIGIACMAASFALLALAGGRAVPVLAAAVLLALSQVFYSPSLDVLVAGDARARGLD
                     LGKTMARQLFWQNLGMMSGSLFAGALFDEAQRRAMPSLAWSIPAACSLAMLVAWTMGM
                     RRAKA"
BASE COUNT          365 a          994 c         1050 g          427 t
ORIGIN      
        1 gattgggacg aggtgcgccg ccacgcctgc gcgagcgatg acgaccatgt catcaagctc
       61 gtgcatgccg cggcgatgca gcacgccgag gcgcccgatg gcgtctggct ggacgcggcg
      121 cgcacggccg tgcgggcctg acgaagctcg ccgcggccta tgtggccatg cagggcgggt
      181 tagccgcagg cgcaacccgc catggcggcg cctcgcaatg ccggcgaacg gcgggttacg
      241 cctgcggcta acccgcccta cggaggcgga ccggcatcat cgcgcgagga actcgcacag
      301 caaccgatgg aactggtgca cgccgcgctc cttgctgggc gagtagcggc cgcggtcgta
      361 gaagcgcgac tgcacgccgc gctgcacctg ctgcacgacc tcctcgtcct ccatctccac
      421 ctggtccagg tcgttgcccg cgccgtcgcc cagcctggcc gcgtcccaca cgtaggagcg
      481 gaagatgacc cgggtgcggt ccatgccgcg cggctgcacg acgttgaccg acagccccca
      541 cgggtagaag ttgagcatca ggttcgggaa gatccagtag tagtacgccg ccacgcgctc
      601 gccgaagtcg ggtgacgaag gcggcagctc gaaggcctgc tcgccgtcgc gggcgcgggc
      661 gaactgcagg ttcgagtggc gttgcagctg gtagtcgtag ccctcgaagc tgagcacctt
      721 gttgagcgcg ggatggatga acgggatgtg caggccctcg agatagttct cgacgtacag
      781 cgcccagtgg gcggcgatgt cgaagttgcg gtcgcgcgcg gggtcgtgcg tgaactcgtg
      841 cagcggcagc caggcgaggc gggtgcgcag gtcgccgagg aaatcgtcca gcggcgcggc
      901 cggccgcacc gccgcgaagg cctggttgcc cagcgtgccg aagggcacgt ggggcaggtg
      961 gtcgctgggt gacgggaagt tcctggcagc gccgaactcg ggcatgaagg tcatgcggcc
     1021 cgcgagatcg aagcggcgcg agtggtagcc gcagcggatc tgctcggcca cgcaaggcgc
     1081 cttcaccagc aggttgccgc ggtgcgtgca gacgttggac aggcagcgca gcgtgcccgc
     1141 gccgtcgcgc gcgagcagca gcggctcggc caggctgccg ggcagcatct cgcgcggcga
     1201 cagcgcgccg ggggcggcga cgtcggcggt gtcgccgatc cattgccagc tgcgcgcgaa
     1261 cacgcgctcg agcacgcgct cgtaggcggc ggggtcgcgg tagaacgcgc tgggcagggt
     1321 gctggcgacg gtgatgtcgg ggtcgacgtg gtacggcgag ctcaaggggt gtctccgtgg
     1381 tgcgcgtcac cgatcatgcc gcgcgtccga ttgcggcgac actgcgggct gacccgattc
     1441 ccccatgacc cgccgcagct ccgccttcaa cgcccgcctc gtgttcacgg gcgtcgcgct
     1501 cgcctcgggc ggcatcgcgt tctacgtgcc gctgatgctg tcggccgtcg gcctcgcgca
     1561 ctcgggcgcc tgggcggcgg gcatcctgtt cggcaccaac ctcggccgcc tggtcggctc
     1621 gcacttggcc agccggcacg gcgtgttcac gcagcggcgc ggggcgatcg tgggcaacat
     1681 cctgctggaa ggcgtcgcct tgttcagcat ggccttcatc gcgtcgcctg cgggcctggt
     1741 cgcagtcgcg acgctggcgg gcctgggcag cgggctgtcg tttcccggca tgaagagctg
     1801 cctgatgcgc ttgcccggcg tggacagcgc gcgcgtcttc gccggcctgt cgctcgcgct
     1861 gcggctgggc atggcgctcg gctacctggc tggcgcgctg gtcggcgcca accacctgac
     1921 cgccgtcttc gccgtgctgc tggtgatgtt cgtcggctac gccggcctca tggacctggc
     1981 gatgcgcgac atcgcgtccg cgcccgccga accgtccgcc acgccggcgc cgcaggccgc
     2041 gcccgcgccg tccgccgacg cgccgctgcc cgtcgggctc acgccggtgc tgctggccaa
     2101 cgccgtgttc tggttcctga cgatccaacc caccgtcacg atgagcctgt acgtgccccg
     2161 gtatgtcccc ggcctggcgg tgtcgaccac ctactgggtc accaccgtca cggtcctgct
     2221 gctgcagctt cgcgtgacgc gcctggcacg cggtatgcca ggccatctcg cgttcctgcg
     2281 catcggcatc gcctgcatgg ccgcgagctt cgcgctgctc gcgctggcgg gcggccgcgc
     2341 cgtgccggtg ctggcggcgg cggtgctgct ggcgctgtcg caggtgttct attcgccgtc
     2401 gctcgatgtg ctcgtcgccg gcgacgcacg cgcccgcggg ctggacctcg gcaagaccat
     2461 ggcccgccag ctgttctggc agaacctggg catgatgagc ggcagcctgt tcgccggcgc
     2521 gttgttcgac gaggcgcagc gccgcgcgat gccctcgctc gcgtggagca tcccggcggc
     2581 gtgttcgctg gcgatgctcg tggcctggac catgggcatg cggcgcgcga aggcctgagc
     2641 cggcccgcgc accacgaccg ccgccagcgc cttggcgatc cacagcacga ggctcgccag
     2701 cgcgcaggcg gctgccgcag cggcgccggc tgtcagcagg ctcacgacca gcatctcgcc
     2761 gacgtccgcg agcaggccgc aggcgaaccc ggaggcggcg cgcacgagcg agcgggccga
     2821 gcggcggccg ggtggc
//
LOCUS       DATCRO010000587         6429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1063840, whole genome shotgun sequence.
ACCESSION   DATCRO010000587 DATCRO010000000
VERSION     DATCRO010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063840"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VIN75_16045"
     CDS             complement(<1..626)
                     /locus_tag="VIN75_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8975732.1"
                     /translation="MPDSATDHRPETGATPRFDTVLVANRGAIACRIIRTLRQLGLRS
                     VAVYSEADADSAHVAQADQAICIGPALAAKSYLDAERILTAARDAGAGAIHPGYGFLS
                     ENPGFADACEAAGIAFVGPTAQQMRAFGLKHTARSLAQSHGVPLLPGSGLLDGLDAAL
                     AEAERIGYPVMLKSTAGGGGIGMRLIRRADEMPAAFEAVERLARANFK"
     gene            complement(700..1362)
                     /locus_tag="VIN75_16050"
     CDS             complement(700..1362)
                     /locus_tag="VIN75_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEY8975733.1"
                     /translation="MDTPVPSTSPSTRDPARAVRDEVVAAGEPWLARIARGQVLRIVD
                     LEGNQAVDTLFFNAQDPAEHYSLTHTIQRQRALYLGLGTTLFSSDDRPMLTIVADTCG
                     RHDTLGGACSCESNTVRYGLGKRFMHSCRDSFMTAIVNDPSGQLTKAHLSSNINFFMN
                     VPVSPTGGLAFADGISGAGKYVELEAHMDVTVLISNCPQLNNPCNAYDPTPVRCLVWD
                     AE"
     gene            complement(1371..2150)
                     /locus_tag="VIN75_16055"
     CDS             complement(1371..2150)
                     /locus_tag="VIN75_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEY8975734.1"
                     /translation="MTSLLEQTPLARAAVPAHSTRFAPELVLWEEEARGGVSWSGALR
                     RGTTLRVTDLEGHANVAVLLFNDDRPSERYNMPDTLKAQHTAYLTRGHACYSDMGRIL
                     CSIPEDTCGWHDTVCGVLDRAAMEQQYGVHRYQEHRNAMHRSGLDGLLVELAKHGLGR
                     RDVHANLNLFSKVRPDATGALHFERGGRAAGQFVDLRCEMNVLVVLSTAPHPLDPAPE
                     YSPGAVRLTAWRSGTAGADDVVRLSCAENARGFINTERSFL"
     gene            complement(2162..2917)
                     /locus_tag="VIN75_16060"
     CDS             complement(2162..2917)
                     /locus_tag="VIN75_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY8975735.1"
                     /translation="MAALAFGASLHAHAEGPEPDQSVTYNIGAVSDYRVRGIAQTSFR
                     PAVQGGIDFADKSGFYAGAWASNVRWVKALNGATRGSVEVDVYGGYRGGLDIAPVTYD
                     AGVITYQYPGNNSGVGGTLPAGTFGNASTVEAYISATWKIVTLKYNRSLTNFMGNLHS
                     HGSQYFDLSASFDLGNSFALVPHVGHQSIPGQADDLGDYTDYSLTLSKDFGNGWSATL
                     AGTGTDAKRAFYTDTNGRFLGKRAVELGAKYTF"
     gene            3407..4156
                     /gene="phbB"
                     /locus_tag="VIN75_16065"
     CDS             3407..4156
                     /gene="phbB"
                     /locus_tag="VIN75_16065"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY8975736.1"
                     /translation="MSNDKRLALVTGGTGGLGQAICKRLSSDGFAVVALHTPGNAHVG
                     AWLAEREREGHAFAAEAVDVGDFDACARVVEKIRGLYGPVAVLVNNAGITQDASFRKM
                     TASQWQQVLRVNLDSMFNMTKPVIDDMLAAGWGRVVNISSVNGQRGAFGQANYAASKA
                     GIHGFTKSLAIEFASKGITVNTVSPGYLRTSMVEKVPEEILRSRILPQIPSGRLGEPD
                     EVARLVAYLVSDGGGYVNGANIAINGGQHMD"
     gene            complement(4197..4658)
                     /locus_tag="VIN75_16070"
     CDS             complement(4197..4658)
                     /locus_tag="VIN75_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975737.1"
                     /translation="MTDARDARDHDCPSAQPDMAGARPFGVVSGTVTETRVAFFKKDA
                     LQHFDWRERFDVPEATQLFRFAARCEKSACGHYDGIDHRCSLGQRVATALPPVTDALP
                     PCLIRPSCRWHAEQGPSVCLRCPQVVTMIPAGDGPLQTVARPEARQAAGAA"
     gene            complement(4661..4972)
                     /locus_tag="VIN75_16075"
     CDS             complement(4661..4972)
                     /locus_tag="VIN75_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975738.1"
                     /translation="MATKKRVETVSLSSLSKSIDKAVSLASEKHGVDFQKGNVILNWE
                     ILGRILRERAALPDGQSRLDVAATITKNVAGIKGQPIVTMVGKDILIGFVERSGTPFG
                     F"
     gene            complement(5072..5878)
                     /locus_tag="VIN75_16080"
     CDS             complement(5072..5878)
                     /locus_tag="VIN75_16080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8975739.1"
                     /translation="MKRLAHALAALVLAAPLARATDSAFHWPHGEKAAVSLSYDDAID
                     SQLDNAIPALDRAGLHGSFYLTLSNASIAKRLAQWRAAAARGHELGNHTLFHYCSARA
                     AGHEWVTPELDLDTVSAARMVAQVRLANTMLQAIDGRTERTFTVPCGDRRAQGGVDYV
                     DLVQGEFVAIKSGSAGVIPDMATLDPMAVGVTTPEGVTGEQLIKLAQDAAARGTMINF
                     TFHGIGGDYITTSKQAHEELLAYLAAHRDVYWTDTFLHIMTYVKAQQASR"
     gene            5989..>6429
                     /locus_tag="VIN75_16085"
     CDS             5989..>6429
                     /locus_tag="VIN75_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975740.1"
                     /translation="MPIVQNSHAGAHYALDIDGLPVESLKTISGLDVQADIATEPPGP
                     DLAPRKHVAGISWSPGRASIGMGMGRSMYAWMKGAFAQGASRASGTLATADVNFRQRS
                     LLAFEEALLTAVTLPRLDAASKESGAFDIEFEPMRVRLAKGDGSD"
BASE COUNT          944 a         2095 c         2365 g         1025 t
ORIGIN      
        1 tccttgaagt tggcgcgcgc caggcgctcg accgcctcga atgcggctgg catttcgtcg
       61 gcgcggcgga tcagccgcat gccgatgccg ccaccgccgg cggtgctctt gagcatcacc
      121 gggtagccga tgcgctcggc ctcggccagc gccgcgtcga ggccgtccag cagcccgctg
      181 ccgggcagca gcggcacgcc gtgcgactgc gcgagcgatc gcgccgtgtg cttgaggccg
      241 aacgcgcgca tctgctgcgc ggtggggccc acgaacgcga tgccggcggc ctcgcaggca
      301 tcggcgaagc cggggttctc ggacaggaag ccatagccgg gatggatggc gccggcacca
      361 gcgtcccgcg ccgccgtgag gatgcgctcg gcgtccaggt agctcttggc ggcgagggcc
      421 gggccgatgc agatcgcctg gtccgcctgg gccacatggg cggagtcggc gtcggcctcc
      481 gagtacaccg cgaccgagcg caggcccagc tggcgcaggg tgcggatgat gcggcaggcg
      541 atggcgccgc ggttggcgac caggacggtg tcgaagcgcg gcgtggcgcc ggtctcggga
      601 cgatgatcgg tggcggagtc gggcatgggg atttctcgga gacgggccgt cccgccggag
      661 gcattgcacg gcgcgggtcg tcccgcgccc ggggcccgtc tactcggcgt cccagaccag
      721 gcagcgcacg ggcgtcgggt cgtaggcgtt gcaggggttg ttcaactgcg ggcagttgga
      781 gatcagcacg gtcacgtcca tgtgcgcctc cagttcgacg tacttgccgg cgcccgagat
      841 gccgtccgcg aacgcgaggc cgccggtcgg cgagaccggc acgttcatga agaaattgat
      901 gttggacgac aggtgcgcct tggtcagctg gcccgacggg tcgttgacga tggcagtcat
      961 gaagctgtcg cggcagctgt gcatgaagcg cttgccgagt ccgtagcgca cggtattgct
     1021 ctcgcacgag caggcgccgc cgagagtgtc gtggcggccg caggtgtccg ccacgatcgt
     1081 cagcatcggg cggtcgtcgc tggagaacag cgtcgtgccg agcccgaggt agagcgcgcg
     1141 ctggcgctgg atcgtgtggg tcaggctgta gtgctcggcg gggtcctgcg cgttgaagaa
     1201 cagcgtgtcg acggcctggt tgccctcgag gtcgacgatg cgcagcacct ggccgcgggc
     1261 aatgcgcgcc agccagggtt cgccggcggc gacgacctcg tcgcgcacgg cacgcgcggg
     1321 atcgcgcgtg ctcgggctcg tggaagggac gggggtatcc atcggaggcc tcacaggaag
     1381 ctgcgttcgg tgttgatgaa gccgcgggcg ttctctgcgc acgagaggcg cacgacgtcg
     1441 tcggccccgg cagttccgga gcgccaggcg gtcaggcgca cggcgcccgg cgagtactcg
     1501 ggtgccgggt ccagcggatg cggcgccgtc gacaggacca caagcacgtt catctcgcag
     1561 cgcaggtcca cgaactgccc ggccgcgcga ccgccgcgtt caaaatgcag tgcaccggtg
     1621 gcgtcggggc gcaccttgct gaacaggttc agattggcat gtacgtcacg ccggccgagg
     1681 ccgtgcttgg ccagttccac cagcaggccg tcgagtccgc tgcgatgcat cgcgttgcgg
     1741 tgctcctggt accggtgcac gccgtactgc tgctccattg cggcgcggtc caggacgccg
     1801 cacacggtgt cgtgccagcc gcaggtgtcc tccggaatcg agcagaggat gcgccccatg
     1861 tcggagtagc aggcgtggcc gcgggtcagg tacgcggtgt gttgggcctt gagcgtgtcg
     1921 ggcatgttgt agcgctcgga gggccggtcg tcgttgaaca gcagcacggc gacgttggcg
     1981 tggccctcca gatcggtgac gcgcagggtg gtgccgcggc gcaacgcgcc ggaccacgac
     2041 acgcccccgc gggcctcctc ctcccacagc acgagttccg gcgcgaaccg cgtggaatgc
     2101 gcgggcaccg cggcgcgtgc caggggcgtc tgttcgagca gcgacgtcat ggaacgcccg
     2161 ctcagaacgt gtacttcgcg ccgagctcga ccgcgcgctt gcccaggaag cggccgttcg
     2221 tgtcggtgta gaacgcgcgc ttggcgtcgg tgccggtgcc tgcgagggtg gcgctccagc
     2281 cgttgccgaa gtccttggac agcgtcagcg aatagtcggt gtagtcgccc aggtcgtcgg
     2341 cctgcccggg gatggattgg tggccgacat gcggcaccag cgcgaagctg ttgcccaggt
     2401 cgaaggaggc gctgaggtcg aagtactggc tgccgtggct gtgcaggttg cccatgaagt
     2461 tcgtcaggct gcggttgtac ttgagcgtga cgatcttcca cgtcgcgctg atgtaggcct
     2521 ccacggtgct cgcgttgccg aaggtgccgg ccggcagggt gccgccgacg cccgagttgt
     2581 tgcccgggta ctggtaggtg atgacgcccg cgtcgtaggt caccggcgcg atgtccaggc
     2641 ccccgcggta gccgccgtag acatcgacct cgacgctgcc gcgcgtcgcg ccgttgaggg
     2701 ccttgaccca gcgcacgttg gacgcccacg cgccggcgta gaagccgctc ttgtccgcga
     2761 agtcgatgcc tccctgcacc gcgggccgga acgaggtctg ggcgatgccg cgcacgcggt
     2821 agtcggaaac cgcgccaatg ttgtacgtca cgctctggtc cggctcgggg ccctcggcgt
     2881 gggcgtgcag actggcgccg aatgccaggg cggccatcag cgtccgggaa atgtggcgga
     2941 cacgggcggg aacacgtcgc agggaggtca tcggattctt tcggatggat tgcgttatta
     3001 ctaatttgat gtttagtact accgacgagc gataagcagc atccgtgcca tgggacatca
     3061 gcctgctgcg ccgggcgcgt ccgtcacccc gcggggtgcg ggtgcaccga tgtgcggcgc
     3121 cgtattggtg ccccctgctc gacgctgggg cgtgcgatca agttgcaagg cggcggccgc
     3181 tgcacatgcc tgcttcctga cgctttcttg gcttggcggc gctggcctcg ggactgaagt
     3241 cgcatgtggt caccgggttg cgctcgacat accgagcccc gtatcacagt agggccttgg
     3301 cgttcgccgg cgttcgagcg acattgccct ggccagacgc cgcgccgctt cgatgtccgc
     3361 agggcggtgt gccgggcgca acgcacgcaa ccgaaggagt ccatggatga gcaatgacaa
     3421 gcgtctcgcc ctcgtcacgg gcggtaccgg cggcctcggg caggccatct gcaagcgtct
     3481 ttccagcgac ggcttcgcgg tcgtcgcgct gcatacgccg ggcaacgccc acgtcggcgc
     3541 ctggctcgcc gagcgcgagc gcgagggtca cgcgttcgcg gccgaggcgg tggacgtcgg
     3601 cgacttcgac gcgtgtgcgc gggtcgtcga gaagatccgc gggctctacg ggccggtggc
     3661 cgtgctggtc aacaacgccg gcatcacgca ggacgcgagc tttcgcaaga tgaccgcttc
     3721 gcagtggcag caggtgctgc gcgtgaacct ggactccatg ttcaacatga ccaagcccgt
     3781 gatcgacgac atgctggccg ccgggtgggg acgcgtggtc aacatctcgt ccgtgaacgg
     3841 ccagcgcggc gcgttcgggc aggcgaacta cgcggcgtcc aaggccggca tccacggctt
     3901 cacgaagtcg ctggccatcg agttcgcgag caagggcatc acggtcaaca cggtgtcgcc
     3961 gggatacctg cgcacgtcca tggtggagaa ggtgcccgag gagatcctgc gcagccgcat
     4021 cctgccgcag atcccgtcgg gccggctggg cgagcccgac gaggtcgcgc ggctcgtcgc
     4081 gtacctggtg tccgacggcg gcggctacgt caacggcgcc aacatcgcca tcaacggcgg
     4141 gcagcacatg gactgagcgg gcgcgcgggc gccgcgcatc gagcgggaat gcccgctcac
     4201 gcggcgccgg cggcctggcg ggcttcgggc cgggccaccg tctgcagcgg cccgtcgccg
     4261 gcggggatca tcgtcaccac ctgcgggcag cgcaggcaca ccgacgggcc ttgctccgcg
     4321 tgccatcgac acgacggccg gatcaggcag ggcggcagcg cgtcggtcac cggcggcagc
     4381 gccgtcgcca cgcgctgtcc cagcgagcag cggtggtcga tgccgtcgta gtggccgcag
     4441 gcggacttct cgcagcgggc cgcgaagcgg aacagctgcg tcgcctcggg cacgtcgaag
     4501 cgctcgcgcc aatcgaagtg ctgcagcgcg tccttcttga agaacgcgac gcgtgtctcc
     4561 gtcaccgtgc cggacacgac cccgaaaggc ctcgcgcccg ccatgtccgg ctgggcgctg
     4621 gggcagtcgt ggtcgcgcgc gtcgcgtgcg tcggtcacgg tcagaatccg aacggcgtgc
     4681 cgcttctctc gacgaagccg atcaggatgt ccttgccgac catcgtcacg atcggctggc
     4741 ccttgatgcc ggccacgttc ttggtgatgg tggcggcgac gtcgagccgg ctctggccgt
     4801 cgggaagcgc cgcgcgctcg cgaaggatgc ggcccaggat ctcccagttc aggatcacgt
     4861 tgcccttctg gaaatccacc ccgtgcttct cgctggcgag gctcacggcc ttgtcgatcg
     4921 atttcgacag cgaggacagg gagacggttt cgacgcgctt cttggtggcc atgggcactc
     4981 ctgctgcgaa gagacggacg gagtgtcctg ccggcgagcc gtcgagcaga tccctagctc
     5041 aggggttcgc attcacgcat cgacgtcgcg ctcaacgcga cgcctgttgc gccttcacgt
     5101 acgtcatgat gtgcaggaac gtgtccgtcc aatagacgtc gcgatgcgcc gcgaggtagg
     5161 cgaggagctc ctcgtgcgcc tgtttcgacg tggtgatgta gtcgccgccg atgccgtgga
     5221 aggtgaagtt gatcatcgtg cccctggcgg cggcgtcctg cgcgagcttg atcaactgct
     5281 cgccagtgac gccttcgggc gtggtgacgc cgacggccat cggatcgagc gtggccatgt
     5341 cggggatgac gccggcgctg ccggacttga tcgcgacaaa ctcgccctgg acgagatcga
     5401 cgtagtcgac gccgccctgc gcgcggcggt cgccgcaggg caccgtgaac gtgcgctcgg
     5461 tgcggccgtc gatcgcctgc agcatcgtgt tggcgagcct gacctgcgcc accatgcgcg
     5521 cggcgctgac ggtgtcgagg tcgagctcgg gcgtgaccca ttcgtggccc gcggccctgg
     5581 ccgagcagta gtggaacaac gtgtggttgc ccagctcgtg gccgcgcgca gcggcggcgc
     5641 gccattgcgc cagccgcttg gcgatcgagg cattcgacag cgtcaggtag aagctgccgt
     5701 gcaggccggc gcgatcgagc gcggggatgg cgttgtccag ctggctgtcg atggcgtcgt
     5761 cataggacag gctgaccgcg gccttctcgc cgtgcggcca gtggaacgcg gaatccgttg
     5821 cacgcgcgag gggggcggcg aggacgaggg cggcaagggc gtgggcgagg cgcttcatgg
     5881 tgcgcgagga tagggcaaat ccacgaattg gggggacggc gacccgccgc ggcgcgtgcg
     5941 acctagcatg gcctttcgca tccgacaaac gtctcgtgga ggtctcgcat gcccatcgtc
     6001 cagaacagcc atgccggcgc gcactacgcg ctcgacatcg acggcctgcc cgtcgaatcg
     6061 ctcaagacca tcagcggcct ggacgtgcag gccgacatcg ccaccgagcc gccgggtccc
     6121 gacctcgcgc cgaggaagca cgtcgcaggc atctcctggt cgccgggccg cgcgagcatc
     6181 ggcatgggca tgggtcggtc catgtacgcg tggatgaaag gcgccttcgc gcagggcgcg
     6241 tcgcgcgcga gcggcacgct ggccacggcc gacgtcaact tccggcagcg ctcgctgctg
     6301 gccttcgagg aggcgctgct gaccgcggtc accctcccgc gcctcgacgc ggccagcaag
     6361 gaatcgggcg cgttcgacat cgaattcgaa ccgatgcgcg tgcgcctggc caagggcgac
     6421 ggctcggac
//
LOCUS       DATCRO010000588         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_287449, whole genome shotgun sequence.
ACCESSION   DATCRO010000588 DATCRO010000000
VERSION     DATCRO010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287449"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VIN75_16090"
     CDS             complement(<1..190)
                     /locus_tag="VIN75_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975741.1"
                     /translation="MSTPALACASLASMSAVSMSLSLDDLPDLPGAREAALDDAPDAL
                     PAQLLEWSEDFELRGIHPC"
     gene            183..932
                     /locus_tag="VIN75_16095"
     CDS             183..932
                     /locus_tag="VIN75_16095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8975742.1"
                     /translation="MLTAQSQVQTAQAQVEGRKADAAQAAAAARQAQAEAANAQQDLA
                     RFKGVDPDAVSRQQYDQAVARARSARAKVDAARATEDTARAQVKAAQADVAVAQAQVA
                     TAQADLESANIQRGYAHVVAPIDGRVTKRAVDVGNVIAIGQPILAIVSDDLWVTANYK
                     ETQLKKMHVGQPVSMEIDAVPGVKFAGHVQSFQRAAGSFFSALPAENATGNYVKVTQR
                     VPVKIVFDHPQELQRYHVGPGMSVKPSVSLE"
     gene            943..2481
                     /locus_tag="VIN75_16100"
     CDS             943..2481
                     /locus_tag="VIN75_16100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8975743.1"
                     /translation="MAAASSASLPRSAAGRHNPWLIAVVVSLATFMEVLDTTIANVSL
                     KHIAGGLGASQDEATWILTSYLISNAIVLPVSGWLATLIGRKKFYMTCVALFTGSSFM
                     CALSPNLGTMIFFRVLQGLGGGGLAPTEQSIFADTFAPAQRAKAFAFYGVTVIVAPAI
                     GPLLGGWLTDSFSWHWIFLINLPVGAAALTPIWFLVDEPQAVKDDTAQFRRNFIPDWL
                     GFLLVAATFGCLQVVLDRFQEDDGFASNLIVAFFIVFVCSAVCLVFWEWFHPQPVFDV
                     KLFRVGSFGAANMVMLLVGITLFSTTQLIPQFAQNLLQYDALAAGKTLALGGVAAAAM
                     MPLAGIAATRLPPWLLVGLGLVCTGLALIHTADLNGQISFYDLSMSRVYQSVCLPFLF
                     VSLTTAGYVGIPPDRNNEASAIINLSRNLGGSIGVAAATTELAWRIQFHHERLGEQLS
                     SIGARGQQVLQHGRAAAEQLVNAQAQVLSYLDIFHVFGVMALIVTPIAFFLKTPAPGE
                     AHGH"
     gene            2471..>3964
                     /locus_tag="VIN75_16105"
     CDS             2471..>3964
                     /locus_tag="VIN75_16105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8975744.1"
                     /translation="MDTEPARPRALARPHVTPARASALACAALVGGCVMGPNFERPHA
                     TPPAAFDSLAGLPADLSGKPVATPYDGTRWWSVFKDPVLDGLVDEARRQNLDLQAAAL
                     RIEEARAQLGAARSQGWPSVSGMGIGGRQRASQNGFFSALSGGGQQQGGSQGGAGAPQ
                     QPAPSPYSDLFQIGFDATWELDLWGKARRGVEAANAGVESAEQARGSALISLTAEVAR
                     TYLNLRGAQHQRQIVEADIADEQQLLRLVESRSRNGFGSKADSVQQQAQLSASQAQLP
                     AIAQAIAQARNGLALLLALPPGALAQRLDAVPAADIALPPAVPVGLPGDLLRRRPDVL
                     KAEADLHAATAKVGQAKAQLFPSITLGGFGGLQSVHASSLTDWASRFWAGGAQVSIPI
                     FEGGKLKAQIRTADVQMKEAALAWRGTVLSAYHDADNALEAYADEQRHAAALARALAD
                     ARRSDELMRSRQRSGFVSMIEVLDAGRQVHQAEQQALQSRVAAATDLV"
BASE COUNT          560 a         1426 c         1417 g          561 t
ORIGIN      
        1 cgcaagggtg gatgccgcgc agctcgaagt cctcgctcca ctcgagcagc tgcgcgggca
       61 gggcatccgg cgcgtcgtcc agggccgcct cgcgcgcgcc cggcaggtcc ggcaggtcgt
      121 cgagcgacag ggacatcgac accgccgaca tcgatgccag gctcgcgcag gccaaggccg
      181 gcgtgctcac ggcgcagagc caggtgcaga ccgcgcaggc gcaggtcgag ggccgcaagg
      241 ccgacgcggc gcaggccgcg gccgcggcgc gccaggcgca ggccgaggcc gccaacgcgc
      301 agcaggacct cgcgcgcttc aagggcgtcg acccggacgc cgtctcgcgc cagcaatatg
      361 accaggcggt ggcgcgggcc cgctcggccc gggccaaggt cgacgcggcg cgcgccaccg
      421 aggacacggc ccgtgcgcag gtcaaggccg cgcaggcgga cgtggcggtg gcgcaggcgc
      481 aggtggccac cgcgcaggcc gacctcgaga gcgcgaacat ccagcgcggc tacgcgcacg
      541 tcgtcgcgcc gatcgacggc cgcgtcacca agcgcgcggt cgacgtcggc aacgtcatcg
      601 cgatcggcca gccgatcctc gcgatcgtca gcgacgacct gtgggtgacg gccaactaca
      661 aggagacgca gctcaagaag atgcacgtcg gccagccggt ctccatggag atcgacgccg
      721 tgccgggcgt gaagttcgcg ggccacgtgc agagcttcca gcgcgccgcg ggcagcttct
      781 tcagcgccct gcccgccgag aacgccaccg gcaactacgt caaggtgacg cagcgcgtgc
      841 cggtcaagat cgtgttcgac catccgcagg aactgcagcg ataccacgtg gggcccggca
      901 tgtcggtcaa gcccagcgtg tcgctcgagt gagggcgcgc ccgtggccgc cgcatcgtcc
      961 gcatcgttgc cgcgctccgc ggcgggcagg cacaacccgt ggctgatcgc ggtggtggtg
     1021 tcgctggcca ccttcatgga ggtgctggac acgaccatcg ccaacgtgtc gctcaagcac
     1081 atcgccggcg gcctgggcgc cagccaggac gaggcgacgt ggatcctcac gagctacctg
     1141 atctccaacg cgatcgtgct gccggtgagc ggctggctgg cgacgctgat cgggcgcaag
     1201 aagttctaca tgacgtgcgt ggcgctgttc accggcagct cgttcatgtg cgcgctgtcg
     1261 cccaacctgg gcacgatgat cttctttcgc gtgctccagg gcctgggcgg cggcgggctc
     1321 gcgccgaccg agcagtcgat cttcgccgac accttcgcgc ccgcgcagcg cgccaaggcc
     1381 ttcgcgttct acggcgtgac cgtcatcgtg gcgccggcca tcggcccgct gctgggcggc
     1441 tggctcaccg acagtttctc gtggcactgg atcttcctga tcaacctgcc ggtgggcgcc
     1501 gcggcgctga cgccgatctg gttcctcgtc gacgagccgc aggccgtcaa ggacgatacc
     1561 gcccagttcc gccgcaactt catccccgac tggctgggtt tcctgctcgt ggccgcgacc
     1621 ttcggctgcc tgcaggtcgt gctcgatcgc ttccaggagg acgacggctt cgcctcgaac
     1681 ctgatcgtcg cgttcttcat cgtgttcgtg tgctcggcgg tctgcctcgt gttctgggag
     1741 tggttccatc cgcagccggt gttcgacgtc aagctgttca gggtgggctc gttcggcgcg
     1801 gccaacatgg tgatgctgct ggtgggcatc accctgttca gcaccacgca gctgatcccg
     1861 cagttcgcgc agaacctgct gcagtacgac gcgctcgccg cgggcaagac gctcgcgctc
     1921 ggcggcgtgg ccgccgccgc gatgatgccg ttggccggca tcgcggccac caggctgccg
     1981 ccgtggctgc tggtgggcct cgggctggtg tgcacggggc tcgcgctgat ccatacggcc
     2041 gacctcaacg ggcagatcag cttctacgac ctgtcgatgt cgcgcgtcta ccagagcgtg
     2101 tgcctgccgt tcctgttcgt gtcgctgacg acggccggct acgtcggcat cccgccggac
     2161 cgcaacaacg aggcgtcggc catcatcaac ctgtcgcgca atctcggcgg ctcgatcggc
     2221 gtggccgcgg ccaccaccga gctcgcgtgg cgcatccagt tccaccacga acgcctgggc
     2281 gagcagttgt cgtcgatcgg cgcgcgcggc cagcaggtgc tgcagcacgg ccgcgctgcc
     2341 gcggaacagc tggtgaacgc gcaggcgcag gtgctcagct acctcgacat cttccatgtg
     2401 ttcggcgtga tggcgctcat cgtcacgccc atcgcgttct tcctcaagac gcccgcgccc
     2461 ggagaagccc atggacactg agcccgcccg cccccgcgcc ctcgcgcgcc cgcacgtgac
     2521 gcccgcgcgc gcgagcgcgc tggcgtgcgc ggcgctcgtg ggtggctgcg tgatggggcc
     2581 gaacttcgaa cgcccgcacg ccacgccgcc cgcggcattc gacagcctgg ccggcttgcc
     2641 ggccgatctg tccggcaagc cggtggccac gccgtacgac ggcacgcgct ggtggtcggt
     2701 gttcaaggat cccgtgctcg acggcctggt cgacgaggcg cggcgccaga acctcgacct
     2761 gcaggcggcc gcgctgcgca tcgaggaggc gcgcgcgcag ctcggcgcgg cccgttcgca
     2821 gggttggcca tcggtcagcg gcatgggcat cggcgggcgc cagcgcgcga gccagaacgg
     2881 gttcttcagc gcgctgtcgg gcggcgggca gcagcaaggc ggatcgcagg gcggcgcggg
     2941 cgcgccgcag cagccggccc cgtcgccgta ctccgacctg ttccagatcg gcttcgacgc
     3001 gacgtgggag ctcgatctct ggggcaaggc gcggcgcggc gtcgaagccg ccaatgccgg
     3061 cgtggaatcg gccgaacagg cacgcggcag cgcgctgatc tcgttgacgg ccgaggtcgc
     3121 gcgcacctac ctgaacctgc gcggggcgca gcaccagcgc cagatcgtcg aggccgacat
     3181 cgccgacgaa cagcagttgc tcaggctggt cgaatcgcgc tcgcgcaacg gcttcggctc
     3241 gaaggccgac tcggtgcagc aacaggcgca gctgtcggcc tcgcaggcgc agctgccagc
     3301 catcgcgcag gccatcgcgc aggcccggaa cggcctcgcg ctgctgctgg cgctgccgcc
     3361 cggggccctg gcccagcgcc tggacgcagt gccggcggcc gacatcgcgc tgccgcccgc
     3421 ggtgccggtg ggcctgccgg gcgacctgct gcgccggcgt cccgacgtgc tcaaggccga
     3481 ggccgacctg cacgccgcca ccgccaaggt gggccaggcc aaggcgcagc tgttcccgtc
     3541 gatcacgctc ggcggcttcg gcgggctgca gtcggtgcac gcaagctcgc tgaccgactg
     3601 ggcctcgcgc ttctgggccg gcggcgcgca ggtctcgatc ccgatcttcg agggcggcaa
     3661 gctcaaggcg cagatccgca ccgcggacgt gcagatgaag gaggccgcgc tcgcctggcg
     3721 cggcacggtg ctgtcggcct accacgacgc cgacaacgcg ctggaggcct acgccgacga
     3781 acagcgccac gccgcggcgc tggcccgggc gctggccgac gcgcgccgca gcgacgagct
     3841 gatgcgctcg cgccagcgca gcggcttcgt ctcgatgatc gaggtgctgg acgccgggcg
     3901 ccaggtgcac caggcggagc agcaggcgct gcagagccgc gtcgcggcgg cgaccgatct
     3961 ggtg
//
LOCUS       DATCRO010000589         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_895316, whole genome shotgun sequence.
ACCESSION   DATCRO010000589 DATCRO010000000
VERSION     DATCRO010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895316"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VIN75_16110"
     CDS             <1..1101
                     /locus_tag="VIN75_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975745.1"
                     /translation="PEAQERLFTPFEQADGSTTRRFGGTGLGLSIARELAQLMGGEIG
                     VESEPGRGSEFWFTARLESAVAPPHAGAPLDAVLDGHGAEQALKARHAGARVLIADDN
                     RINQELATELLRLVDLKVEVADNGRTAVDMARRGAFDMILMDMQMPEMDGLEATRRIR
                     AEPRLDATPIVAMTASAFGADRDACLAAGMNDHIGKPVNPAALYDKMLRWLDESRKAP
                     RRAPVDPPAPPPPRAAPQPTDVLEGIEALDVVRGMSLFAGQRALYMQALGYFVDLYGD
                     GLAAVDKFLAGAPGSSREAAGREIHSMGGAAAALGAIELENAAHRIDKMMRGERARLV
                     DDEQLRPELQAVRDAVADLVARLGAALGRSAT"
     gene            complement(1094..1561)
                     /locus_tag="VIN75_16115"
     CDS             complement(1094..1561)
                     /locus_tag="VIN75_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEY8975746.1"
                     /translation="MPSLAATLSALAAAAAVHAEPIGDVNTAFQWIGPDHKIVVEAYD
                     DPKVAGVTCYVSRAKTGGIKGALGVAEDKAEASIACRQVGPVSVAKPLPQQEEVFSER
                     ISLVFKRLRIVRMVDRQRNTLVYLTYSDRLVDGSPKNSVTAVPIDRATPIPLR"
     gene            complement(1637..>2937)
                     /locus_tag="VIN75_16120"
     CDS             complement(1637..>2937)
                     /locus_tag="VIN75_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY8975747.1"
                     /translation="SRTAMNADAHPTVPAPLHEAYQAMLAAADPRRVLARHLPPPPAG
                     RTVVVGLGKAAAAMAQAVESQWTGPLEGVVVVPEGAALPLARLRIVESSHPVPDERSV
                     RGARALLDAVQGLGPDDLVLALVSGGGSALCAWPVDGVSLQDKQALTRALLASGASIH
                     EMNLVRAQLSAIKGGRLAAAAWPARVATLLISDIPGDDPSLVASGPTLGTAGTRADAH
                     EVLRRHGIAPGPAIAQALSGDANPAIQPDDPRLSRNTCIVVASAWDGLQAASQAVRAR
                     GLACHVLGDALEGETRDLAIAHAGIVDAILRHGQPFEAPCMLLSGGEATVTLRGNGRG
                     GRNTEFALAFGLAVERSGSAPHVHLLSAGTDGLDGRAGAAGAWLAPDTLGRARALGLS
                     PRDHLDRNDSASLFDRIDGLVRTGPTHTNINDFRAILIAP"
BASE COUNT          391 a         1063 c         1106 g          377 t
ORIGIN      
        1 cccgaggcgc aggagcggct gttcacgccg ttcgagcagg ccgacggctc gacgacccgc
       61 cgcttcgggg gcaccgggct gggcctgtcg atcgcgcgcg agctggcgca gctgatgggt
      121 ggagagatcg gcgtcgagag cgagccgggc cgcggcagcg agttctggtt caccgcccgg
      181 ctggaatcgg ccgtcgcacc gccgcacgcg ggggcgccgc tcgacgccgt gctcgacggc
      241 cacggcgcgg aacaggcgct caaggcgcgc catgccggcg cgcgcgtgct gatcgccgac
      301 gacaaccgca tcaaccagga gctcgccacg gagctgctgc gcctggtcga cctgaaggtg
      361 gaggtggcag acaacggccg caccgcggtc gacatggcgc gccgcggcgc cttcgacatg
      421 atcctgatgg acatgcagat gcccgagatg gacggcctgg aggcgacgcg ccggatccgc
      481 gccgagccgc gcctggacgc cacgcccatc gtggcgatga ccgccagcgc gttcggtgcc
      541 gatcgcgacg cgtgcctggc cgccggcatg aacgaccaca tcggcaagcc ggtgaatccg
      601 gcggcgctct acgacaagat gctgcgctgg ctggacgagt cgcgcaaggc gccgcggcgc
      661 gcgccggtcg atccgcccgc gcccccgccg ccgcgggccg cgccgcagcc caccgacgtg
      721 ctcgaaggca tcgaggcgct cgacgtggtg cgcggcatga gcctgttcgc cggccagcgc
      781 gcgctgtaca tgcaggccct gggctacttc gtcgacctgt acggcgacgg cctggccgcg
      841 gtcgacaagt tcctcgccgg cgcgcccggg agctcgcgcg aggccgcggg ccgcgagatc
      901 cactcgatgg gcggcgccgc cgcggcgctc ggtgccatcg agctggagaa cgcggcgcac
      961 cgcatcgaca agatgatgcg cggcgagcgc gcccgcctcg tcgacgacga gcaactgcgg
     1021 cccgagctgc aggccgtgcg cgacgccgtc gccgacctcg tcgcgcgcct gggcgccgcg
     1081 ctgggccggt ccgctacctg agcgggatcg gcgtggcgcg gtcgatcgga actgccgtca
     1141 ccgagttctt cggcgacccg tcgaccaggc ggtccgagta ggtcaggtag acgagcgtgt
     1201 tgcgctggcg gtcgaccatg cgcacgatgc gcagccgctt gaacacgagg ctgatgcgct
     1261 cgctgaagac ctcctcctgc tgcggcagcg gcttggcgac gctgacgggc ccgacctgcc
     1321 ggcacgcgat cgaggcctcg gccttgtcct cggcgacgcc cagcgcgccc ttgatgccgc
     1381 ccgtcttggc gcgcgacacg tagcaggtca cgcccgccac cttcgggtcg tcgtaggcct
     1441 cgacgacgat cttgtggtcg ggcccgatcc actggaatgc ggtgttgacg tcgccgatgg
     1501 gctcggcgtg gacggccgcg gcggcggcaa gcgccgacag ggtggcggca agagagggca
     1561 tcgggcgcat gcgtttcctt caagggcggt gcggagccct gaactctagt gggtcgtggc
     1621 tgtcctgcgg gcgccgtcat ggcgctatca ggatcgcgcg gaagtcgttg atgttggtgt
     1681 gcgtgggccc cgtgcggacc aggccgtcga tccggtcgaa gaggctggcg ctgtcattgc
     1741 gatcgagatg gtcgcgcgga gacaggccca gcgcgcgggc gcgtcccagc gtgtccgggg
     1801 ccagccatgc gccggccgcg ccggcccggc cgtcgaggcc atcggtgccc gccgacagca
     1861 ggtgcacgtg cggcgccgag cccgagcgct ccaccgccag cccgaacgcc agcgcgaact
     1921 cggtgttgcg gccgccgcgc ccgttgccgc gcagcgtgac cgtggcttcc ccgccggaca
     1981 acagcatgca gggggcctcg aatggctggc cgtggcgcag gatggcgtcg acgatgccgg
     2041 catgcgcgat cgccaggtcg cgtgtctcgc cctcgagtgc gtcgcccagc acatggcagg
     2101 ccagcccgcg cgctcgcacg gcctgcgacg cggcctgcaa gccgtcccag gcgctggcca
     2161 ccacgatgca cgtgttgcgc gacagccgcg ggtcgtccgg ttggatggcg ggattcgcgt
     2221 cgcccgacag tgcctgcgca atggcggggc ccggcgcaat cccgtggcgc cgcaggacct
     2281 cgtgggcatc ggcgcgcgtg ccggccgtgc ccagcgtcgg gcccgatgcc acgagcgacg
     2341 ggtcgtcgcc ggggatgtcg gagatcagga gcgtggccac ccgcgccggc cacgccgcgg
     2401 ccgccagtcg gccgcccttg atggccgaca gctgcgcgcg cacgaggttc atctcgtgga
     2461 tggatgcgcc gctggccagc aatgcgcgcg tcaaggcctg cttgtcctgc agcgagacgc
     2521 cgtcgacggg ccaggcgcac agggcggaac caccgccgga gaccagcgcc agcaccagat
     2581 cgtccggccc caacccctgc acggcgtcga gcagcgcccg cgcgccgcgc acgctgcgct
     2641 catccggcac ggggtggctg gactcgacga tgcgcaggcg cgcaagcggc aaggccgcgc
     2701 cctcgggcac gacgaccacg ccttcgagcg gccccgtcca ttgcgattcc acggcctgcg
     2761 ccatcgcggc cgccgccttg cccaggccga ccacgacggt gcgccctgcc ggcggcggcg
     2821 gcaggtggcg cgccagcacc cggcgcgggt ccgccgcggc gagcatggcc tggtacgcct
     2881 cgtggagcgg ggccggcacg gtgggatgcg cgtctgcgtt catggcggtc cgggacg
//
LOCUS       DATCRO010000590         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_501698, whole genome shotgun sequence.
ACCESSION   DATCRO010000590 DATCRO010000000
VERSION     DATCRO010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501698"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VIN75_16125"
     CDS             <1..972
                     /locus_tag="VIN75_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975748.1"
                     /translation="RAWTTGVCREPGETPGARTPARADRVLKPLWTRDLGTEPTQLAT
                     SAPCAGAARDASVFATRDGGLWLDQGTQVAKLDPVTGTTSTALPTPRSKNVCSVVTPA
                     GTGFLNAGGDTLTWRPLAAATDPARRRVVERRPGWTATLLPVRGDVARVAWTSPARDV
                     VIADYDANGKRLREAATTLAAFGDDPAGPASTPPAPCQDAHGTPIAIGYDWRAGPFGS
                     QRGSTCGPLPGMARLVWWSGASIAPRTDRSAPLQRAQPSIDGTIAVSADDTQLHVVNL
                     ALQREIAQVDLGDGFDGRTWVLASRKLVLLEATGSDGHRRLKAYALP"
     gene            complement(1027..2637)
                     /locus_tag="VIN75_16130"
     CDS             complement(1027..2637)
                     /locus_tag="VIN75_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY8975749.1"
                     /translation="MLWIGIHLPALSLESFVATLAPELPAGEQLVDDLGDLDDIDLVG
                     PVALIAQHRIVAANDAARACGVRPGQKRATALGLAPQLRLGEADPGRDEAALMAVAHV
                     LMGFTPSVSFGGDHVVLAEVQASLRYFGGLPSLIERLRAELAPLGHVLRLACAPTPQG
                     AQWMAVGGDDPGFVDDKVRWRARVGAVPVDRMAAAAPHLAALGTLGLATAADLWRQPR
                     AGLTRRFGGQLLADIDRALGDQPDPRTPLQPAPVFDSRLELFMRADDSAALLTGAAVL
                     LNRLTAWATAHHGLVGAFELRLHHESRRRMIEDPSLRTTKLDIGLGEPVADTAHLHSL
                     LRERLQRLPLAFSVIEMSLHCHSITPGAPPNTELFPSAASVGEGWNRLLERFEARLGA
                     HNIQRLQPVADHRPERATAVRAAGDAAPSRARAAGMPLNLAPPVNAAAAASGDPAHWP
                     RLRRPVWLLRQPQPLRDEAGTPLLAGKPLWLVAGPERIESGWWDGGYAARDYFIAQDH
                     EGALLWVYRFRHAPEVDQTGWFLQGRFG"
     gene            complement(2651..>3004)
                     /locus_tag="VIN75_16135"
     CDS             complement(2651..>3004)
                     /locus_tag="VIN75_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975750.1"
                     /translation="EQALRSGQAGAVVAWTPASLKGEALRRLQLAAQSHDGPAFVLRA
                     AAAAAQPSPAPLRLALAGAGPDAMAVHVLKRRGPALEEPVLLALPPVLSRAARQRALT
                     PRRTRATVRALSTIA"
BASE COUNT          377 a         1134 c         1148 g          345 t
ORIGIN      
        1 cgtgcctgga cgacaggcgt ctgccgcgag ccgggcgaga cgccgggcgc ccgcacgccg
       61 gcgcgtgccg accgcgtgct caagccgctg tggacgcgcg atctgggcac cgagccgacg
      121 cagctcgcga cgtcggcacc gtgcgccggc gccgcgcgcg atgcctccgt gttcgccacc
      181 cgcgacggcg gcctgtggct cgaccagggc acgcaggtcg ccaagctcga tcccgtcacg
      241 ggcacgacgt ccacggcgct gcccacgccg cgttcgaaga acgtctgcag cgtcgtgacg
      301 cctgccggca cgggctttct caacgccggc ggcgacacgc tcacgtggcg cccgctggcc
      361 gccgccactg acccggcgcg ccgccgcgtc gtcgaacgcc gcccgggctg gacggccacg
      421 ctgctgccgg tgcgcggcga tgtcgcgcgg gtggcctgga cgtcgccggc gcgcgacgtc
      481 gtcatcgccg actacgacgc caatggcaag cgcctgcgcg aagccgcgac gacgctggcc
      541 gccttcggcg acgatcccgc cggcccggcc tcgacgcccc ccgcgccctg ccaggacgcg
      601 cacggcacgc cgatcgccat cggctacgac tggcgcgccg gcccgttcgg ctcgcaacgc
      661 ggcagcacct gcggcccgct gcccggcatg gcgcgcctcg tctggtggag cggcgcatcg
      721 atcgccccgc gcaccgaccg ctccgcgccg ctgcaacgcg cgcagccgtc catcgacggc
      781 accatcgccg tgtcggccga cgacacgcaa ctgcacgtcg tcaacctggc gctgcaacgc
      841 gagatcgcgc aggtcgacct cggcgacggc ttcgacgggc gcacgtgggt gctggcgtcg
      901 cgcaagctgg tgctgctgga ggcgacgggc agcgatgggc atcgcaggtt gaaggcgtat
      961 gcgttgccgt gatcgttcga aggccttgga cgccggaccc cggtggctgg cacgcctgcg
     1021 atggcgctac ccgaaccgcc cctgcaggaa ccaccccgtc tggtcgacct ccggcgcatg
     1081 ccggaagcgg tagacccaca gcagcgcgcc ttcgtggtcc tgcgcgatga agtagtcgcg
     1141 tgccgcgtag ccaccgtccc accagccgga ctcgatgcgc tcggggccgg cgaccagcca
     1201 cagcggcttg ccggcgagca gcggcgtgcc ggcctcgtcg cgcagcggct gcggctggcg
     1261 cagcagccac accggacgcc tcaggcgcgg ccagtgcgcc gggtcgcccg aggccgcggc
     1321 ggccgcgttc acgggcgggg ccaggttcag cggcatgccg gccgcgcgag cgcgcgacgg
     1381 tgcggcgtcg ccggccgcgc gcacggcggt ggcgcgttcg gggcgatggt cggccaccgg
     1441 ttgcaggcgc tggatgttgt gcgcgccgag ccgcgcctcg aagcgctcga gcagccggtt
     1501 ccagccctcg ccgacgctgg cggccgacgg gaacagctcg gtgttgggcg gcgcgccggg
     1561 cgtgatcgag tggcagtgca ggctcatctc gatcaccgag aaggccagcg gcaggcgctg
     1621 cagccgctcg cgcagcagcg agtgcaggtg cgcggtgtcg gccaccggct cgcccaggcc
     1681 gatgtccagc ttcgtggtgc gcagcgacgg atcctcgatc atcctgcgcc ggctctcgtg
     1741 atgcagccgc agctcgaacg cgccgaccag gccgtggtgc gcggtggccc aggcggtgag
     1801 gcgattgagc agcaccgcgg cgccggtgag cagcgcggcg ctgtcgtcgg cgcgcatgaa
     1861 cagctccagc cggctgtcga agacgggcgc gggctgcagc ggggtgcgcg ggtcgggctg
     1921 gtcgccgagc gcgcggtcga tgtcggccag cagttggccg ccgaagcggc gcgtgaggcc
     1981 ggcgcgcggc tgccgccaca ggtcggcggc ggtggccagg cccagcgtgc cgagcgcggc
     2041 caggtgcggc gcggccgcgg ccatgcgatc gacgggcacg gcaccgacgc gcgcgcgcca
     2101 gcgcaccttg tcgtcgacga agcccgggtc gtcgccaccc acggccatcc attgcgcgcc
     2161 ctgcggcgtg ggcgcgcagg ccaggcgcag cacgtggccc agcggtgcga gctcggcgcg
     2221 cagccgctcg atgaggctcg gcagcccgcc gaagtagcgc aggctggcct gcacctcggc
     2281 cagcaccacg tggtcgccgc cgaacgagac gctgggcgtg aagcccatca gcacgtgggc
     2341 gacggccatc agcgcggcct cgtcgcgccc cgggtcggcc tcgcccaggc gcagctgcgg
     2401 cgccagcccg agcgcggtgg cccgcttctg gccgggccgc acgccgcacg cgcgcgccgc
     2461 gtcgttggcc gcgacgatgc ggtgctgcgc gatcagcgcg accgggccga cgaggtcgat
     2521 gtcgtcgagg tcgccgaggt cgtcgaccag ttgctcgccg gccggcagct ccggcgccag
     2581 cgtggcgacg aaggattcca gcgacaacgc cggcaggtga atgccaatcc acagcatgat
     2641 ggacgttcca tcaggcgatc gtggacaggg cccgcaccgt cgcgcgcgtg cgccggggcg
     2701 tgagcgcgcg ctggcgcgcg gcgcgcgaga gcaccggcgg cagcgcgagc agcaccggct
     2761 cctccagcgc cgggccacgg cgcttgagca cgtgcacggc catcgcgtcg ggacccgcgc
     2821 cggcgagcgc caggcgcagc ggcgccgggc tgggctgggc ggccgccgcg gcggcgcgca
     2881 gcacgaaggc cgggccgtcg tgcgactgcg cggccagttg cagccgacgc agcgcctcgc
     2941 ccttgaggct ggccggcgtc cacgcgacga ccgctcccgc ctggccgctg cgcaaggcct
     3001 gctc
//
LOCUS       DATCRO010000591         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_456646, whole genome shotgun sequence.
ACCESSION   DATCRO010000591 DATCRO010000000
VERSION     DATCRO010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456646"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..>3050
                     /locus_tag="VIN75_16140"
     CDS             554..>3050
                     /locus_tag="VIN75_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8975751.1"
                     /translation="MKLQRHPIAAAAALALLGALAPAQVLAQAADAAAAPASAASAPA
                     AAKEDVQRVEVTGLRASLERSLAQKRNADSVVEVVTADDIGKLPDKNVADALQRVPGV
                     NIASSAGGEGGFSENDRVSIRGTSPSLTQTLVNGHMISSGDWFVLDQEGGSLGRSVSY
                     ALMPSEILGSAVVHKSATADLVEGGVAGAVDMITRRPLDFKKQLTVEGSIQAIYSDLP
                     ARTDPQANALVAWKNEASTFGALLQVFSETRHERRDGQEIFGYGAIDPIWAAAVAHPD
                     LANVNAPSGINSALFEQTRKRQGGLVDLQLKPMQDLTLDLNGFYSHLAASNYNRSFYN
                     QPLNNLMIGIVPSSYKVVNGTLVQATFDKSSYPVPSATQDPNLWAEAPATVDQIYRPG
                     ASATSSYLDLGAQWRVTDMLKLNGLVGTTKGEGRTPRDIGYEADFLNSGMSYQFNGSG
                     IATVAFPGGDPAHFSAANVTTGAAWGSKVQTEDKENYGQIDADLALDAGVFDSLKFGA
                     RFANHSRDVELPQASGCSVCDGTTNAPLPNWSGQTYPGNFGNGLGGDILKDVWQLSRG
                     DVLNWANQYDPLQPSDTSEVWSGEMRLKERDTAAYAMANLVGSKWRGNVGVRLVRTHE
                     SSTSNLPADLYPDQTPQGVNNQNGEFVPVTTEHTFTNVLPSANLRFDVTKDLVARASM
                     ARVMSRPDYGALVSAVNLDDNLHTGSGGNPDLKPILSTNSDLSLEWYYAPRALLSVGL
                     FYMDMSSYVAYGNIKRDYVDRSHTLPGQPPLIATYTLSVPTNVGATNKGIELGWQQTF
                     GVVGGELNYTYTDGKVTDGSQMVGNSKNTYNVVA"
BASE COUNT          541 a         1106 c          990 g          413 t
ORIGIN      
        1 acgccgacct gcgcgcgccg actgcgcagg aagcgcagta cgacctggac gggctcgacc
       61 atctgctcga acgcagcgtg ctcaactttc ccgaccaccg cgaattcctg gaagacattc
      121 cggcggccgt gaccgagaag acgttccagg tctatcagtg gtgagtcggg cgcggcggcg
      181 tgcgatcggg ggcacgctcc cgccgcgccg tcgcgatcca cgacgcgcgc tgcggccgcc
      241 tcacgacccg gacccggccc gaccgcgcgg ctgacgaggc gcgcgacgtc gccggcgagt
      301 cgcgtgggcg atctgccgcg acgcatgttc ccccagccga gacgccgcaa gctccggatc
      361 acctgcggtg ccaggcggtg gcggcggggt cgcccgagca attcgccgat cggcacgcga
      421 tgcgcgcacc gaacggcgag ccctgcggca tgcgcggcgc caattctcag tgaaaaccag
      481 cgatacgcgc ccaggtcgac ctcgtatcat cctctcatta aatcaactca tttgatttga
      541 gggaaacagg cgcatgaagc tccaacgtca tccgatcgcc gccgccgcgg ccctggccct
      601 gctcggcgcc ctcgcgccgg cgcaggttct cgcccaagcc gccgacgccg ccgcggcgcc
      661 ggcctcggcc gcgtccgcgc cggccgccgc caaggaggac gtccaacgcg tcgaggtgac
      721 cgggctgcgc gcctcgctcg aacgctcgct cgcgcagaag cgcaatgccg actcggtggt
      781 cgaggtggtc acggccgacg atatcggcaa gctcccggac aagaacgtcg ccgacgcgct
      841 gcagcgcgtg ccgggcgtca acatcgcgtc ctcggccggc ggcgaaggcg gcttctcgga
      901 gaacgaccgc gtgtcgatcc gcggcaccag cccgagcctg acgcagacgc tggtcaacgg
      961 ccacatgatc tcgtcgggcg actggttcgt cctcgaccag gaaggcggct cgctgggccg
     1021 cagcgtcagc tacgcgctga tgccgtccga gatcctgggc agcgccgtcg tccacaagag
     1081 cgcgacggcc gacctcgtcg aaggtggcgt cgccggcgcc gtcgacatga tcacgcgccg
     1141 gccgctcgac ttcaagaagc agctgaccgt cgagggctcg atccaggcga tctacagcga
     1201 cctgcccgcc aggacggacc cgcaggccaa cgcgctcgtc gcatggaaga acgaggcctc
     1261 gacgttcggc gcgctgctgc aggtgttcag cgagacccgc cacgagcgcc gcgacggcca
     1321 ggagatcttc ggctacggcg cgatcgaccc gatctgggcc gcggcggtgg cgcatccgga
     1381 cctggccaac gtcaacgcgc cctccggcat caactccgcg ctgttcgagc agacgcgcaa
     1441 gcgccagggc ggcctggtcg acctgcagct caagccgatg caggacctca cgctcgacct
     1501 gaacggcttc tactcgcacc tggccgcatc caactacaac cgcagtttct acaaccagcc
     1561 gctgaacaac ctgatgatcg gcatcgtgcc gagcagctac aaggtggtca acggcacgct
     1621 ggtgcaggcc accttcgaca agtcgtccta ccccgtgccc agcgccacgc aggatccgaa
     1681 cctgtgggcc gaggcgccgg ccaccgtcga ccagatctac cgcccgggcg cgagcgccac
     1741 gagcagctac ctcgatctcg gcgcccaatg gcgcgtgacc gacatgctca agctcaacgg
     1801 cctggtcggc acgaccaagg gcgagggccg cacgccgcgc gacatcggct acgaggccga
     1861 cttcctcaat tccggcatga gctaccagtt caatggctcg ggcatcgcca cggtggcctt
     1921 cccgggcggc gatccggcgc acttcagcgc ggccaacgtc accaccggcg cggcgtgggg
     1981 ctcgaaggtg cagaccgagg acaaggagaa ctacggccag atcgacgccg acctcgcgct
     2041 cgacgcgggc gtgttcgact cgctgaagtt cggtgcgcgc ttcgcgaacc actcgcgtga
     2101 cgtcgaactg ccgcaggcct cgggctgctc ggtgtgcgac ggcaccacca acgcgccgct
     2161 gcccaactgg tcgggccaga cctatccggg caatttcggc aacggcctgg gcggcgacat
     2221 cctcaaggac gtctggcagc tgagccgcgg cgacgtcctg aactgggcca accagtacga
     2281 ccccctgcag ccgtccgaca ccagcgaggt gtggagcggc gagatgcgcc tgaaggaacg
     2341 cgacaccgcc gcctacgcga tggccaacct ggtcggctcc aagtggcgcg gcaacgtggg
     2401 cgtgcggctg gtgcgcacgc atgaatcctc gacctcgaac ctgccggccg acctgtaccc
     2461 cgaccagacg ccgcagggcg tgaacaacca gaacggcgag ttcgtgccgg tgaccaccga
     2521 gcacaccttc accaacgtgc tcccgagcgc caacctgcgc ttcgacgtca cgaaggacct
     2581 ggtggcgcgc gcgtcgatgg cacgggtgat gtcgcggccc gactacggcg cgctcgtgag
     2641 cgcggtcaac ctcgacgaca acctgcacac cggctcgggc ggcaaccccg acctcaagcc
     2701 gatcctgtcg acgaactccg acctgtcgct cgagtggtac tacgcgccca gggcgctgct
     2761 gtcggtcggc ctgttctaca tggacatgtc gtcgtacgtg gcctacggca acatcaagcg
     2821 cgactacgtc gaccgcagcc acacgctgcc tggccagccg ccgctgatcg cgacgtacac
     2881 gctgtcggtg ccgaccaacg tgggcgcgac caacaagggc atcgagctgg gctggcagca
     2941 gaccttcggc gtggtcggcg gcgagctgaa ctacacgtac accgacggca aggtcaccga
     3001 cggcagccag atggtgggca actccaagaa cacgtacaac gtcgtggcct
//
LOCUS       DATCRO010000592        10721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1030391, whole genome shotgun sequence.
ACCESSION   DATCRO010000592 DATCRO010000000
VERSION     DATCRO010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030391"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VIN75_16145"
     CDS             <1..236
                     /locus_tag="VIN75_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975752.1"
                     /translation="PRRALARYLASYAWFLPPLALAAALKLPSAWAIFGLVAGWIVLY
                     ALAALLHPRRQFWHDALCGTAIVTARPCAPLPQ"
     gene            260..838
                     /locus_tag="VIN75_16150"
     CDS             260..838
                     /locus_tag="VIN75_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8975753.1"
                     /translation="MFSLCVYCGSRNGDDPRYAEAARALGTALGEGGHRLVYGGGHAG
                     LMGAVADATLAAGGQVLGVIPRRLVARELGHRGIQELRVVDTMHERKLQMAQAADAFV
                     ALPGGLGTLEELFEAWTWQQLGYHARPIGLLDAAGFYRPLLEMLAHTRDAGFVDAAQV
                     NRLRVDTDARRLLQTLRDEAQASPAGKDFNPV"
     gene            complement(913..1251)
                     /locus_tag="VIN75_16155"
     CDS             complement(913..1251)
                     /locus_tag="VIN75_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8975754.1"
                     /translation="MKQITAIIKPFKLEEVREALGEVGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVKESDVDRAIEAIVKAAKTGKIGDGKIFVTAVEQVVRI
                     RTGETDESAV"
     gene            complement(1297..3018)
                     /locus_tag="VIN75_16160"
     CDS             complement(1297..3018)
                     /locus_tag="VIN75_16160"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8975755.1"
                     /translation="MSEPDSVRVALLQINPTVGDLDGNARLIADAARAAWQQGARVAL
                     APELALCGYPPEDLLLRPAFLQANAAALEALAHSLADCEGLHLVVGHPWDGGAAGHGD
                     DVRSKSVSVPRVFNAASVLAGGRVAGTYCKRELPNYQVFDERRYFVSGRDAGLSPVVF
                     ESGGTKFGVLVCEDAWFHEPALLARHAGAQVLCVINASPFHLGKPADREAQMAHRARA
                     TGLPLLYAHMTGGQDEVVFDGGSFALDEHGEVVARAALFDAGTLMVDVAPGRAPAPAA
                     GSAVVPLPEPEAQAWAALVTGVRDYIGKNGFPSVLIGLSGGIDSALVLAIAVDALGTD
                     KVRTVMMPSPYTADISWIDARDMAERLGVRHDEIAIAPMFDAFRHALAPQFAGLAEDT
                     TEENIQARVRGTLLMALSNKTGAIVLTTGNKSEMATGYCTLYGDMAGGFAVIKDVAKT
                     LVYRLAEWKNRQPTRRADGSVGPVIPERIITRPPSAELRPDQTDQDSLPPYEILDAIL
                     AAYMEENVAIDDIVAAGFERTDVERVTRLIKINEYKRRQSPVGIRITHRGFGRDWRYP
                     ITSRFRA"
     gene            3041..4294
                     /locus_tag="VIN75_16165"
     CDS             3041..4294
                     /locus_tag="VIN75_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975756.1"
                     /translation="MTENDYVIEVHPGVGAVGAHEWDELLSAQARPTPFMSHAYLRAM
                     ETSGSVGGDTGWEPATLVVRERAGGTLVAAAAAYLKTHSYGEYVFDWAWADAYRRYGV
                     PYYPKLLVAVPFTPVPGSRLLARDAPARALLLRALGALARETGCSSVHLLFGDDADYD
                     AARGAGWMMRQGVQFHWHNRHDGAPSDDQSASVVQPYAGFSEFLADLQRDKRKKIAQE
                     RRYVAEAGVTFETIEGAQMTGADWDFFHACYRNTYAQHFSTPYLTREFFTAMHESMPD
                     NWVLFVASREGKRVACSLIAVDRAAGAAFGRYWGALEPVPHVHFEACYYQPLQWCIEN
                     RIRRFEGGAQGEHKMARGLMPARTQSAHWIAEPEFARAIGAHLKQEGGAIDEYLSELD
                     DRSPFRPASPAPAPAPAAPGEQRSR"
     gene            complement(4230..4808)
                     /locus_tag="VIN75_16170"
     CDS             complement(4230..4808)
                     /locus_tag="VIN75_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8975757.1"
                     /translation="MTADPSLDRRAARSRDLLLAALPGLIAERGFERLTTQNIIDRAG
                     VGRATFYAHFENKEELLAASVGRLRAWLEAMRRPAPGQPFAFMPPFFDHLDSHRAIYR
                     TTFERESEVSVERMIRAMLRELVRAELVAQRGGRRDDAAIELATQFVVSTFWSVTVWW
                     LGGGIDRTPAEINAIFVRLAQPGLELALATPA"
     gene            4937..5680
                     /locus_tag="VIN75_16175"
     CDS             4937..5680
                     /locus_tag="VIN75_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8975758.1"
                     /translation="MHLTDSPALVRLRGATRRHPLATGEFRALDGVDLDVRAGECVAV
                     VGPSGSGKTTLAHLLTGIEQPTDGSVVVDGQPLHALSQDALAEWRGRRVGIVFQFFQL
                     LPTLTIAENVMLPMDLRATWPRRERRPRALALLERLRIADQADKLPAALSGGQQQRAA
                     IARALANDPALIVADEPTGNLDSATALEVMGLLAALARDGKTVIVVTHERELGRFFTR
                     TVTLRDGRVLADTAAPAAPHASAHAEARA"
     gene            5677..8070
                     /locus_tag="VIN75_16180"
     CDS             5677..8070
                     /locus_tag="VIN75_16180"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8975759.1"
                     /translation="MIAPRWIKLWRDAVAARGRLALIVVAVAASVAALATMAVAGAVL
                     RREVPLSYVSSNPASAQLELSAPPDDALLDRIRHRPEVAQADAAATLRGRIEFAPGEQ
                     APLLLFVVPDLATLSINTVHPEAGAWPPADGTLLVERSALPLTRHAIGSTIALELRHG
                     GRRVVPLAGTVHDPGVAPAGQEQTVYGYVTPRTLAAWGEAVPMTLLKIVVARDAGDAA
                     AIERAMRRLSAWLATQGCAVEQIRIPAPGRHPHQGQMNAILAMLTGFSALALVLGAVL
                     CATVIGAWLAQQVRQIAIMKAIGARTRQVAGTTLALVATLGAVAMAIGLPAGVAAGNA
                     FVQAISQLLNLRLQDPTPPTALVAALAVPGIALPTLAALGPVLAAARRSVRAALDDHG
                     AAPAHSAFAQAVARINLGSLAPTLALRNALRRRTRLVLTLGLLACAGAMLETTLAVRA
                     AWTDDVARAAQQRRWDLELALPPQAGPDRVAALLRPLPDVRAVESWDAAGAAAASDGV
                     EVVHTYPDGGHGGFTLRAAPAATAMIAPRLVQGRWLKAGDDGVVVLNTMARAVAFHDA
                     RVGDTVTLSIAHRPVALRLVGVIDETLTPGAAYVSPSTFARVAGGDATGVLRVALRER
                     DADRPAQAAITQALARAGIAPRGAITARRFAAAQGGHVAILVAALGFIALLMAVVGLL
                     GLASALSVATAERTREFGVLRALGASRGLALRVVLAEGAVIGVASWAAAVVLALPLSA
                     VVARVLASIAAQELAPRLYPGAVALLLPALLAGAVAASLAPALRASRWTVRQSLALA"
     gene            complement(8282..8833)
                     /gene="ppa"
                     /locus_tag="VIN75_16185"
     CDS             complement(8282..8833)
                     /gene="ppa"
                     /locus_tag="VIN75_16185"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY8975760.1"
                     /translation="MSLHLVSPGAKAPEEFNVIIEIPMNADPIKYEVDKESGALFVDR
                     FMTTAMHYPCNYGYVPKTLSDDGDPVDVLVITPFPLTPGVVVTCRAIGVLKMDDEAGG
                     DAKLLAVPTTKILPMYSHWTKPEDINQMRLNAIQHFFEHYKDLEPNKWVKVKGWEGPD
                     AAKAEITSGIQAYEAEQAKKAGK"
     gene            9086..>10721
                     /locus_tag="VIN75_16190"
     CDS             9086..>10721
                     /locus_tag="VIN75_16190"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8975761.1"
                     /translation="MSTTLALEIALACGVLAVLYGFFQRAWILKQDAGNARMQEIAAA
                     VQQGAAAYLSRQYRTIAIVGVVLAIIIAYFLGAKTAGGYVLGAVLSGACGFIGMNVSV
                     RANVRTAQAATHGIGPALNVAFKGGAITGMLVVGLGLLGVGLFYWFISGGTAADAATL
                     KPLLGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTLIATMTLGALMMAGNALSAVVYPLMLGGVSIVASIIGC
                     FAVKASPGMKNVMPALYKGLAVAGVLSLIAFWFVTKAVMSDDALGAGTQMRLFGACAV
                     GLILTGLLVWITEYYTGTDYKPVQHVAQASTTGHGTNIIAGLGVSMKSTAWPVLLVCV
                     AIFVSYNLAGLYGIAVAATSMLSMAGIVVALDAYGPITDNAGGIAEMAELPDSVRDIT
                     DPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHGLEGRGLHATFDLSDHKVILGLF
                     IGGLIPYLFGAMAMEAVGRAAGAVVVEVRKQFADGAI"
BASE COUNT         1423 a         3883 c         3818 g         1597 t
ORIGIN      
        1 cgccccgccg cgcccttgcc cgctacctgg cctcctatgc gtggttcctg ccaccgctgg
       61 ccctggccgc cgcgctgaag ctgcccagcg cctgggccat cttcggactc gtcgcgggct
      121 ggatcgtgct gtacgcgctc gccgccctgc tccacccgcg gcgccagttc tggcacgacg
      181 cgctgtgcgg cacggccatc gtcacggcgc ggccttgcgc gccgctgccg caatgacgcc
      241 ccccggcgac aatcgcccga tgttctcgct ctgtgtctat tgcggctcgc gcaacggcga
      301 cgacccgcgc tatgccgagg ccgcgcgcgc gctcggcacg gccctcggcg agggcggcca
      361 ccggctggtc tacggcggcg gccatgcggg cctgatgggg gcggtggccg acgccacgct
      421 ggccgccggc ggccaggtgc tgggcgtcat accgcggcgg ctggtcgcgc gcgagctggg
      481 ccatcgcggc atccaggaac tgcgcgtggt cgacaccatg cacgagcgca agctgcagat
      541 ggcgcaggcg gccgacgcct tcgtcgccct gcccggcggc ctgggcacgc tggaggagct
      601 gttcgaggcc tggacgtggc agcaactggg ctaccacgcg cgcccgatcg gcctgctgga
      661 cgccgccggc ttctaccgtc cgctgctgga gatgctcgcg cacacgcgcg acgccggctt
      721 cgtcgacgcc gcccaggtga accgcctgcg cgtggacacc gacgcgcggc gcctgctgca
      781 gacgctgcgc gacgaggcac aggcgagccc ggcgggcaag gacttcaatc ccgtctgaag
      841 gcgcaacccg gggcagccgt gcgcggccac ccccggcttc gtcatcctgc gcgcagtcgc
      901 aggatccggc gttcagaccg ccgactcgtc ggtctcgccg gtacggatgc gcacgacctg
      961 ctcgaccgcg gtcacgaaga tcttgccgtc gccgatcttg cccgtcttgg ccgccttgac
     1021 gatggcctcg atcgcgcggt cgacgtcgct ttccttgacg accacctcca ccttgacctt
     1081 gggcaggaag tcgaccacgt actccgcgcc gcggtacagc tcggtgtggc ccttctggcg
     1141 gccgaagccc ttgacctcgg tgaccgtcag gccggacacg ccgacctcgc cgagcgcctc
     1201 gcgcacttcc tcgagcttga acggcttgat gatggcggtg atctgtttca tggtgggctc
     1261 cggagggttg aaggcgtgaa ggacggatga atcgatttaa gcacggaagc gcgaggtgat
     1321 gggatagcgc cagtcacgcc cgaaaccgcg gtgcgtgatg cggatgccca ccggcgactg
     1381 ccggcgcttg tactcgttga tcttgatcag gcgcgtgacg cgctccacat cggtgcgttc
     1441 gaagccggcg gcgacgatgt cgtcgatcgc gacgttctcc tccatgtacg cggccaggat
     1501 cgcgtcgagg atctcgtagg gcggcaggct gtcctggtcg gtctggtcgg ggcgcagctc
     1561 ggccgagggc ggccgcgtga tgatgcgctc ggggatgacc gggcccacgc tgccgtccgc
     1621 gcggcgcgtg ggctggcggt tcttccactc ggccaggcgg tagaccagcg tcttggcgac
     1681 atccttgatg accgcgaagc cgccggccat gtcgccgtac agcgtgcagt agccggtggc
     1741 catctcgctc ttgttgccgg tggtcagcac gatcgcgccg gtcttgttcg acagcgccat
     1801 cagcagggtg ccgcgcacgc gggcctggat gttctcctcg gtggtgtcct cggccaggcc
     1861 ggcgaactgc ggcgccagcg cgtggcggaa ggcgtcgaac atcggcgcga tggcgatctc
     1921 gtcgtggcgc acgcccaggc gctcggccat gtcgcgcgcg tcgatccacg agatgtcggc
     1981 cgtgtagggc gagggcatca tcacggtgcg caccttgtcg gtgccgagcg cgtcgacggc
     2041 gatggcgagc accagcgcgg agtcgatgcc gcccgacagg ccgatgagca cgctcgggaa
     2101 accgttcttg ccaatgtagt cgcgcacgcc ggtgaccagc gcggcccagg cctgcgcctc
     2161 gggctcgggc agcggcacga cggcgctgcc ggcggccggc gccggcgcgc ggccgggcgc
     2221 gacgtcgacc atcagcgtgc cggcatcgaa cagcgcggcg cgcgcgacga cctcgccgtg
     2281 ctcgtcgagc gcgaacgagc cgccgtcgaa caccacctcg tcctggccgc ccgtcatgtg
     2341 cgcgtacagc agcggcaggc ccgtggcgcg ggcgcggtgc gccatctgcg cctcgcggtc
     2401 ggccggcttg cccaggtgga acggcgaggc gttgatgacg cacagcacct gcgcgccagc
     2461 gtgcctggcc agcagcgcag gctcgtggaa ccaggcgtcc tcgcacacca gcacgccgaa
     2521 cttcgtgccg ccggactcga aaacgaccgg cgagaggccg gcgtcgcggc ccgagacgaa
     2581 gtagcggcgc tcgtcgaaca cctggtagtt gggcagctcg cgcttgcagt aggtgcccgc
     2641 gacgcgtccc cccgcgagca ccgaggccgc gttgaacacg cgcggcacgc tcacggactt
     2701 cgacctaaca tcgtcgccat gacccgcggc cccgccgtcc cacggatgac cgaccaccag
     2761 atgcagcccc tcgcaatcgg ccagcgagtg cgccagcgcc tcgagcgccg cggcgttggc
     2821 ctgcaggaac gccgggcgca gcaggagatc ctcgggcggg tagccgcaca gcgccagttc
     2881 gggcgcgagc gcgacgcggg cgccctgctg ccaggcggcg cgcgcggcat cggcgatcag
     2941 gcgcgcattg ccgtcgagat cgccgacggt cgggttgatc tggagcagtg cgacccgcac
     3001 ggaatctggc tcggacatga ctggaagcac tggaagcgcg ttgactgaaa acgattatgt
     3061 catcgaggtt caccccggcg tcggtgcggt cggggcgcac gagtgggacg aactgctgtc
     3121 cgcgcaggcc cggcccacgc cgttcatgtc ccacgcgtac ctgcgggcga tggagacctc
     3181 gggcagcgtg ggcggcgaca ccggctggga accggcgacg ctggtcgtgc gcgaacgggc
     3241 gggcggcacg ctcgtggccg ccgcggccgc gtatctcaag acgcattcgt acggcgagta
     3301 cgtgttcgac tgggcctggg ccgacgccta ccgccgatac ggggtgccgt actacccgaa
     3361 gctgctggtc gcggtgcctt tcacgcccgt cccgggctcg cgcctgctcg cgcgtgacgc
     3421 ccccgcgcgc gcgctgctgt tgcgcgcgct cggcgcgctg gcgcgcgaga cgggctgttc
     3481 gtcggtgcac ctgctgttcg gcgacgatgc ggactacgac gcggcgcgcg gggccggctg
     3541 gatgatgcgc cagggcgtgc agttccactg gcacaaccgg cacgacggcg caccttccga
     3601 tgaccagtcc gcgtccgtcg tgcagcccta cgccggcttt tccgagttcc tggccgacct
     3661 gcagcgcgac aagcgcaaga agatcgcaca ggagcggcgc tacgtcgccg aggcgggcgt
     3721 cacgttcgag acgatcgaag gcgcgcagat gaccggcgcg gactgggact tcttccacgc
     3781 ctgctaccgc aacacctacg cccagcattt ctcgacgccc tacctgacgc gcgagttctt
     3841 caccgcgatg cacgagtcga tgccggacaa ctgggtgctg ttcgtcgcct cgcgcgaggg
     3901 caagcgcgtc gcgtgctcgc tgatcgccgt cgaccgcgcg gccggcgccg cgttcggccg
     3961 ctactggggc gcgctcgagc ccgtcccgca cgtgcacttc gaggcctgct actaccagcc
     4021 gctgcagtgg tgcatcgaga acaggatccg ccgcttcgag ggcggcgcgc agggcgagca
     4081 caagatggcg cgcggcctca tgccggcgcg cacgcagtcg gcgcactgga tcgccgagcc
     4141 cgagttcgcg cgcgccatcg gcgcccacct gaagcaggaa ggcggcgcga tcgacgagta
     4201 cctgagcgag ctcgacgacc gctcgccgtt caggccggcg tcgccagcgc cagctccagc
     4261 cccggctgcg ccaggcgaac aaagatcgcg ttgatctcgg ccggcgtgcg gtcgatgccg
     4321 ccgccgagcc accacacggt caccgaccag aaggtgctca cgacgaactg cgtcgccagc
     4381 tcgatggccg cgtcgtcgcg ccggccgccg cgttgcgcga cgagctcggc gcgcaccagc
     4441 tcgcgcagca tcgcgcggat catgcgctcg acggacacct cgctctcgcg ctcgaaggtc
     4501 gtgcggtaga tcgcgcgatg gctgtccagg tggtcgaaga acggcggcat gaaggcgaac
     4561 ggctggcccg gcgcgggccg gcgcatggcc tcgagccagg cgcgcagccg gccgacgctg
     4621 gcggccagca gttcctcctt gttctcgaaa tgggcgtaga acgtcgcccg gccgacgccg
     4681 gcgcgatcga tgatgttctg cgtggtcagg cgttcgaagc cgcgctcggc aatcaggccg
     4741 ggcaaggcgg ccaggagcag gtcgcgcgag cgggcggcac ggcggtcgag cgaagggtcg
     4801 gcggtcacgg cggcgggtga tgtcgaacag cgcgcggcga tctgttcggt agtgaacgac
     4861 gggatgcgcg ctgttcgggc ggcgcgcgct gtcgaacagt atgttcggct tctcgtcgca
     4921 tcgcaacgcc tccctcatgc atctcaccga ctcccctgcc ctcgtgcgcc tgcgtggcgc
     4981 cacccgccgc catcccctgg ccaccggcga attccgcgcg ctcgatggtg tcgacctcga
     5041 cgtgcgcgcc ggcgaatgcg tcgccgtcgt cgggccctcg ggcagcggca agacgacgct
     5101 cgcgcacctg ctgaccggca tcgagcagcc gaccgacggc agcgtcgtcg tcgacggcca
     5161 gccgctgcac gcgctgtcgc aggacgcgct ggccgagtgg cgcgggcgcc gcgtgggcat
     5221 cgtgttccag ttcttccagc tgttgcctac gctgaccatc gccgagaacg tgatgctgcc
     5281 gatggacctg cgcgccacct ggccgcgccg cgagcgccgg ccgcgcgccc tcgcgctgct
     5341 ggagcggctg cgcatcgccg accaggccga caagctgccg gccgcgctgt ccggcggcca
     5401 gcagcagcgc gcggccatcg cgcgcgcgct ggccaacgat ccggcactga tcgtcgccga
     5461 cgaacccacc ggcaatctcg actcggccac cgcgctggag gtgatgggcc tgctggccgc
     5521 gctggcacgc gacggcaaga cggtgatcgt cgtgacgcac gagcgcgagc tgggccgctt
     5581 cttcacgcgc accgtcacgc tgcgcgacgg ccgcgtgctg gccgacacgg ccgcgcccgc
     5641 ggcgccgcac gccagcgcgc acgcggaggc ccgcgcatga tcgcgccgcg ctggatcaag
     5701 ctgtggcgcg acgccgttgc agcacgcggt cggctggcgc tgatcgtcgt cgcggttgcg
     5761 gccagcgtcg ccgcgctcgc gacgatggcg gtcgccggcg cggtgctgcg gcgcgaggtg
     5821 ccgctcagct acgtgagctc caacccggcc tccgcgcagc tggagctgtc ggcgccgccg
     5881 gacgacgcgc tgctcgatcg catccgccac cgccccgaag tcgcgcaggc ggacgccgcg
     5941 gccacgctgc gcggccgcat cgagttcgcg ccgggcgagc aggcgccgct gctgctgttc
     6001 gtcgtgcccg acctcgccac gctgtcgatc aacaccgtgc accccgaggc gggcgcgtgg
     6061 ccgccggccg acggcacgct gctggtcgaa cgcagcgcgc tgccgctcac gcgccacgcg
     6121 atcggcagca cgatcgcgct cgagctgcgg cacggcggcc ggcgcgtcgt gccgctcgcg
     6181 ggcaccgtcc acgacccggg cgtcgcgccg gccggccagg aacagaccgt ctacggttac
     6241 gtcacgccgc gcacgctcgc agcctggggc gaggcggtgc ccatgacgct gctgaagatc
     6301 gtggtggcgc gcgacgccgg cgacgcggcc gcgatcgagc gcgcgatgcg ccgcctgtcc
     6361 gcctggctcg cgacgcaggg ctgcgccgtc gaacagatcc gcatcccggc gccgggccgc
     6421 catccgcacc agggccagat gaacgcgatc ctcgcgatgc tgaccggctt cagcgccctc
     6481 gcgctggtgc tgggcgctgt gctgtgcgcc accgtcatcg gcgcgtggct cgcgcagcag
     6541 gtgcgccaga tcgcgatcat gaaggccatc ggcgcgcgca cgcgccaggt cgcgggcacc
     6601 acgctcgcgc tggtcgcgac gctcggcgcg gtcgcgatgg ccatcggact gcccgcgggc
     6661 gtcgcggccg gcaacgcgtt cgtccaggcg atctcgcaac tgctcaacct gcgcctgcag
     6721 gatcccacgc cgcccacggc cctggtcgcg gcgctcgccg tgcccggcat cgcgctgccc
     6781 acgctggccg cgctggggcc ggtgctggcc gccgcccgcc gcagcgtgcg cgccgcgctc
     6841 gacgaccacg gcgcggcgcc cgcgcactcg gcgttcgcgc aggccgtcgc gcgcatcaac
     6901 ctgggctcgc tcgcgccgac gctggccctg cgcaacgcgc tgcgccgccg cacccggctc
     6961 gtgctgacgc tgggattgct ggcgtgcgcg ggggcgatgc tcgagacgac gctggccgtt
     7021 cgcgcggcct ggacggacga cgtcgcgcgc gccgcgcagc agcggcgctg ggacctcgag
     7081 ctcgcgctgc cgccgcaggc cgggcccgac cgggtcgccg cgctgctgcg cccgctgccc
     7141 gacgtgcggg ccgtcgaatc ctgggacgcg gcaggcgccg cggcggccag cgacggcgtg
     7201 gaggtcgtgc acacctaccc cgacggcggc cacggcggtt tcacgctgcg cgcggcgccg
     7261 gcggccacgg cgatgatcgc gccgcgactc gtgcaaggcc gctggctgaa ggccggcgac
     7321 gacggcgtcg tcgtgctcaa cacgatggcc cgcgcggtcg cgttccatga cgcgcgggtg
     7381 ggcgacacgg tcacgctgtc gatcgcgcac cggccggtcg cactccgcct ggtgggcgtc
     7441 atcgacgaga cgctgacgcc cggcgcggcc tacgtgagcc cgtccacgtt cgcgcgggtc
     7501 gcgggcggcg acgccacggg cgtgctgcgc gtcgcgctgc gcgaacgcga cgccgaccgc
     7561 cccgcgcagg ccgcgatcac gcaggccctg gcgcgcgcgg gcatcgcgcc gcgcggcgcg
     7621 atcaccgcgc gccgcttcgc ggcggcccag ggcgggcacg tggccatcct cgtggccgcg
     7681 ctcgggttca tcgcgctgct gatggcggtg gtcggcctgc tgggcctggc ctcggcgctg
     7741 tcggtggcca cggccgagcg cacgcgcgag ttcggcgtgc tgcgcgcgct cggcgcgagc
     7801 cgcgggctgg cgctgcgcgt cgtgctggcc gagggcgccg tgatcggcgt ggcgagctgg
     7861 gccgcggcgg tcgtcctggc gctgccgctg tcggccgtcg tcgcgcgcgt gctcgcctcg
     7921 atcgccgcgc aggagctggc acctcgcctg taccccggcg ccgtcgcgct gctgctgccc
     7981 gcgctgctgg ccggcgcggt cgcggccagc ctggcgccgg cgttgcgcgc ctcgcgctgg
     8041 acggtgcggc aatcactcgc cctcgcctga aattcccttt gaccgccaag gaaatcccat
     8101 gaacaagtcc cgcgccctgc gcctcgtcct cgcgatcgcc gtgctcgccg ccatcgtcgt
     8161 cgccctgcac gtcttcgacc cgatcggcct gatccgccgc ctgcacggcc tggattgagt
     8221 ccccggaaac ggcgaagccc tcgcgcggag ggcttcgtgc tcttccagcg cagggccagg
     8281 cttacttgcc ggccttcttc gcctgctcgg cctcgtaggc ctggatgccc gaggtgatct
     8341 cggccttggc ggcgtccggg ccttcccagc ccttgacctt gacccacttg ttcggctcga
     8401 ggtccttgta gtgctcgaag aagtgctgga tcgcgttcag gcgcatctgg ttgatgtcct
     8461 ccggcttggt ccagtgcgag tacatcggca ggatcttggt ggtgggcacc gccagcagct
     8521 tggcgtcgcc gcccgcctcg tcgtccatct tcagcacgcc gatcgcgcgg caggtgacga
     8581 ccacgcccgg cgtcagcggg aacggggtga tgaccagcac gtcgaccggg tcgccgtcgt
     8641 ccgacagcgt cttgggcacg tagccgtagt tgcacgggta gtgcatggcc gtggtcatga
     8701 agcggtcgac gaacagcgcg ccgctctcct tgtccacttc gtacttgatc gggtcggcgt
     8761 tcatcgggat ctcgatgatc acgttgaatt cttcgggcgc cttggcgccg ggggaaacga
     8821 ggtgcaggct catgatgctc tattttgagg cagggtcgcg ggaatacccg gattctacgg
     8881 ggtcggcggt accgccctgg tacagccacg aagctgacac gtcggtcagc aagagttgcg
     8941 ccaccgagga aaacaccatg tcggagcgac gtgagcgacc gctaggatcg cccggcgacc
     9001 ttgcctgcaa gattgactga agacgtaagc cgaaccacgc agtgaggcgc atccgatcca
     9061 cacaacggga aggagatcac ttttcatgtc aacgactctg gcgctcgaaa tcgcgctcgc
     9121 ctgcggcgta ctggccgtgc tctacgggtt cttccagcgc gcctggatcc tgaagcagga
     9181 cgccggcaat gcccgcatgc aggagatcgc ggcagccgtc cagcaaggag ctgcagccta
     9241 cctgtcgcgc cagtaccgca cgatcgccat cgtcggggtc gtgctggcga tcatcattgc
     9301 gtacttcctc ggcgccaaga ccgccggtgg ctacgtgctg ggcgccgtgc tgtcgggcgc
     9361 ctgcggcttc atcggcatga acgtctccgt gcgcgcgaac gtgcgcaccg cgcaggcggc
     9421 cacccacggc atcggcccgg ccctgaacgt cgcgttcaag ggcggcgcca tcaccggcat
     9481 gctggtggtc ggcctgggcc tgctgggcgt gggcctgttc tactggttca tcagcggcgg
     9541 caccgcggcc gacgcggcca ccctcaagcc gctgctgggc ttcgcgttcg gctcgtcgct
     9601 gatctcgatc ttcgcgcgac tgggcggcgg catcttcacc aagggcgccg acgtcggcgc
     9661 cgacctggtg ggcaaggtcg aggccggcat ccccgaggac gacccgcgca acccggccgt
     9721 gatcgccgac aacgtgggcg acaacgtcgg cgactgcgcc ggcatggccg ccgacctgtt
     9781 cgagacctac gccgtcacgc tgatcgccac gatgacgctg ggcgcgctga tgatggccgg
     9841 caacgcgctg tccgcggtcg tgtacccgct gatgctgggc ggcgtgtcga tcgtcgcgtc
     9901 catcatcggc tgcttcgcgg tcaaggcctc gccgggcatg aagaacgtga tgcccgcact
     9961 gtacaagggc ctcgcggtgg cgggcgtgct ctcgctgatc gccttctggt tcgtcaccaa
    10021 ggccgtgatg tccgacgacg cgctcggcgc cggcacgcag atgcgcctgt tcggcgcctg
    10081 cgccgtcggg ctgatcctga ccggcctgct ggtgtggatc accgagtact acaccggtac
    10141 cgactacaag ccggtgcagc acgtggcgca ggcctcgacc accggccacg gcaccaacat
    10201 catcgcgggc ctgggcgtgt cgatgaagtc caccgcctgg ccggtgctgc tggtgtgcgt
    10261 ggcgatcttc gtgtcgtaca acctcgcggg cctgtacggc atcgcggtcg cggccacgtc
    10321 gatgctgagc atggccggca tcgtcgtcgc gctcgacgca tacggcccga tcaccgacaa
    10381 cgccggcggc atcgccgaga tggcggagct gcccgacagc gtgcgcgaca tcaccgaccc
    10441 gctggacgcc gtgggcaaca cgaccaaggc cgtcaccaag ggctacgcga tcggctcggc
    10501 cggcctcgcg gcgctggtgc tgttcgcgga ctacacgcac ggcctggagg gccgcggcct
    10561 gcacgccacg ttcgacctgt ccgaccacaa ggtcatcctg ggcctcttca tcggcgggct
    10621 gattccctat ctgttcggcg ccatggccat ggaagccgtc ggccgcgctg ccggtgccgt
    10681 cgtcgtcgag gtgcgcaagc agttcgccga cggcgccatc a
//
LOCUS       DATCRO010000593         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_738139, whole genome shotgun sequence.
ACCESSION   DATCRO010000593 DATCRO010000000
VERSION     DATCRO010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738139"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VIN75_16195"
     CDS             <1..884
                     /locus_tag="VIN75_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8975762.1"
                     /translation="GQPCARWSLDYRFPDPDEVLAELRQRFSAIRRKLGPDARLRLQG
                     VGLAAPLSLSSWQALLGVTPAVAERWNRLDLRDAIADLCDWPVHSMKDTAAACVAELV
                     AGRGRGMHSFLYIFVDTFIGGGLVIDSHLRNGLTGNAGAIGSMPLGLPDARGPAPQLL
                     SVASLHTLEQAWRDAGLADPGFDGPEALQAPWRAVTLAWVDKAATAIAHAINGAVCLL
                     DLDGVIIDGALSRELLDALIAATSEAMRRSNWEGVSAPQLLAGTVGPDAGALGGALLP
                     LHANFAPDRDLFIKEHA"
     gene            881..1612
                     /locus_tag="VIN75_16200"
     CDS             881..1612
                     /locus_tag="VIN75_16200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975763.1"
                     /translation="MTTTPFAGRVAVVTGAASGIGLASATALVAAGARVVMVDRDADA
                     LRRACTPHGDAMIPLEIDLLDAAACASLVPRSLALAGRVDILHANAGLYIGGDLVDAR
                     TDAIDRMLHLNVNVVMKNVHDVLPHMIERGAGDIIVTSSLAAHYPTPWEPVYASSKWA
                     VDCFVQTTRRQVFRHGIRVGAISPGPVMTGLIADWPADKLREARASGSLLEPAEVADV
                     VLFMLTRPRGMTIRDVVMMPSNFDL"
     gene            complement(1822..2250)
                     /locus_tag="VIN75_16205"
     CDS             complement(1822..2250)
                     /locus_tag="VIN75_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975764.1"
                     /translation="MTIDVMMNEDKPRPVRAAVARTLLALAVAAAGAGCVAPNTTVHR
                     ISVDVQAHPKDLRACVIEAIRGVDGVPGVNPATQGIEAASVSFRTTLPEVTGVVERQG
                     DDVRVSVEVMAFVEPSNFWEFGQSRTKIIADAIVARCGAL"
     gene            complement(2321..2611)
                     /locus_tag="VIN75_16210"
     CDS             complement(2321..2611)
                     /locus_tag="VIN75_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975765.1"
                     /translation="MLCNCSICRRNGALWSFYAIDEVTISGHPEHTTGYEFGPRTITT
                     FHCRHCGCATHWEPLKAGATKLAFNMRNFDPDELGDVRLRRFDGAVTWAYLD"
     gene            2794..>3024
                     /locus_tag="VIN75_16215"
     CDS             2794..>3024
                     /locus_tag="VIN75_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975766.1"
                     /translation="MRRLLLFWRQGGRDLKLLWRALRHPDRPAWLMPATVVLAFFALD
                     PLNLALPLMGAVDDLVLLPLALSALVGLLPRHL"
BASE COUNT          406 a         1103 c         1070 g          445 t
ORIGIN      
        1 gcggccagcc gtgcgcgcgc tggtcgctgg actaccgctt ccccgacccc gacgaggtgc
       61 tggccgagct gcgccagcgc ttcagcgcga tccgccgcaa gctcgggccc gatgcgcgac
      121 tgcgcctgca gggcgtcggc ctcgccgccc cgctgtcgtt gagcagctgg caggcgctgc
      181 tgggcgtgac gcccgcggtg gccgagcgct ggaaccggct cgacctgcgc gacgccatcg
      241 ccgacctgtg cgactggccg gtgcattcga tgaaggacac ggcggcggcc tgcgtcgccg
      301 agctcgtcgc cgggcgcggc cgcggcatgc acagcttcct ctacatcttc gtcgacacct
      361 tcatcggcgg cggcctggtg atcgacagcc acctgcgcaa cggcctgacc ggcaacgccg
      421 gcgcgatcgg ctcgatgccg ctgggcctgc ccgacgcgcg cggcccggcg ccgcagctgc
      481 tcagcgtcgc ctcgctgcac acgctggagc aggcgtggcg cgacgccggc ctcgccgacc
      541 ccggcttcga cggccccgag gcgctgcagg cgccctggcg cgcggtcacg ctcgcgtggg
      601 tggacaaggc cgccaccgcg atcgcgcatg ccatcaacgg cgccgtgtgc ctgctggacc
      661 tggacggcgt gatcatcgac ggcgcgctct cgcgcgagct gctggacgcg ctcatcgccg
      721 cgacgtccga ggcgatgcgg cgctccaatt gggaaggcgt gagcgcgccg cagctgctgg
      781 ccggcacggt cggccccgac gcgggcgcgc tcggcggcgc gctgctgccg ctgcacgcga
      841 acttcgcgcc cgaccgcgac ctgttcatca aggagcacgc atgaccacga cgccgttcgc
      901 gggccgggtg gccgtggtca cgggcgcggc gtcgggcatc ggcctcgcca gcgcgacggc
      961 gctcgtcgcg gcgggtgcgc gcgtcgtgat ggtcgaccgc gacgccgacg cgctgcgccg
     1021 cgcgtgcacg ccgcatggcg acgcgatgat cccgctggag atcgacctgc tcgacgcggc
     1081 ggcctgcgcg tcgctggtgc cgcgctcgct cgcgctcgcc ggccgcgtgg acatcctgca
     1141 cgccaacgcc gggctgtaca tcggcggcga cctcgtcgac gcgcgcaccg acgcgatcga
     1201 ccgcatgctg cacctgaacg tcaacgtcgt gatgaagaac gtgcacgacg tgctgccgca
     1261 catgatcgag cgcggcgcgg gcgacatcat cgtcaccagc tcgctggccg cgcactatcc
     1321 gacgccctgg gagccggtct acgcgtcttc gaagtgggcc gtcgactgct tcgtgcagac
     1381 gacgcggcgc caggtgttca ggcacggcat ccgcgtgggc gcgatctcgc cggggccggt
     1441 gatgacgggc ctgatcgccg actggccggc cgacaagctg cgcgaggcgc gcgcgtccgg
     1501 cagcctgctg gagccggccg aggtggccga cgtcgtgctg ttcatgctga cgcggccgcg
     1561 cgggatgacg atccgcgatg tcgtgatgat gccgtcgaac ttcgacttgt gacattcatc
     1621 tgcatgaccg cccgtggggc gggttagccg caggcgtaac ccgccgttcg caggcgttgc
     1681 taccgtgcgc cgacgttgcg acagggcacc ggcattgcga cggggcaccg gcactgcgac
     1741 agggcgacat ggcgggttac gcctgcggcc aacccgccct gcaccgccct cgcacatgac
     1801 gatgcgtcga gggtccggcg ttcagagcgc cccgcaacgc gccacgatcg cgtccgcgat
     1861 gatcttcgtg cgcgactgcc cgaactccca gaagttcgac ggctccacga acgccatcac
     1921 ctcgacgctg acgcgcacgt cgtcgccctg gcgttccacg acgccggtga cctcgggcag
     1981 cgtcgtgcgg aacgagacgc tggccgcctc gatgccctgc gtcgccggat tgacccccgg
     2041 cacgccgtcg accccgcgga tggcctcgat cacgcaggcg cgcaggtcct tcggatgcgc
     2101 ctgcacgtcc accgagatgc ggtgcacggt ggtgttcggc gcgacgcagc cggcaccggc
     2161 cgccgccacg gccagggcca gcagcgtcct ggcgacggcg gcccgcacgg gtcgcggctt
     2221 gtcttcgttc atcatcacat cgatggtcat gcggacattc tcgccgcgcc ggactgccat
     2281 ttcggcgcag cggcagaaat gaaagtgccg gcggccgccg tcagtcgagg taggcccacg
     2341 tgaccgcccc gtcgaagcgc cgcagccgca cgtcgcccag ttcgtccgga tcgaagttgc
     2401 gcatgttgaa ggcgagcttg gtcgcaccgg ccttcagcgg ctcccagtgg gtcgcgcagc
     2461 cgcagtgccg gcagtggaag gtggtgatcg tgcgcggccc gaattcgtag ccggtggtgt
     2521 gctccggatg gcccgagatc gtcacctcgt cgatggcgta gaacgaccac agcgcgccgt
     2581 tgcggcggca gatggagcag ttgcacagca ccagagcgtc ggggcgggcg gggacggaca
     2641 ggcggacctt tccgcagtgg catgaaccgt cgagcatctt cgagacatct ccagcgttgc
     2701 ggcgattctc gctcgacaag aaggcacgct tgaagcgacg cccgtcgccc ccagatgcgg
     2761 acgacgggcc cgtccgcccg caaggagccc cgcatgcgcc gactcttgct gttctggcgc
     2821 cagggcggcc gcgatctgaa gctgctgtgg cgcgcgctgc ggcacccgga tcgccctgcc
     2881 tggctgatgc ccgccaccgt cgtgctggcg ttcttcgcgc tcgacccgct gaacctcgcg
     2941 ctgccgctga tgggcgcagt cgacgacctc gtgctgctgc cgctcgcgct gagcgcactc
     3001 gtcggactgc tgccgcgcca cctg
//
LOCUS       DATCRO010000594         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_96437, whole genome shotgun sequence.
ACCESSION   DATCRO010000594 DATCRO010000000
VERSION     DATCRO010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96437"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..2808)
                     /locus_tag="VIN75_16220"
     CDS             complement(226..2808)
                     /locus_tag="VIN75_16220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY8975767.1"
                     /translation="MSKSLVIAEKPSVAQDIVRALTPVAGKFEKHAEHFENDKYVVTS
                     AVGHLVEIKAPEEYEVKRGKWSFAHLPVIPPHFELAPIDKAKTRLNAVVKLVKRKDVT
                     ELINACDAGREGELIFRLIVQYAGIDKKSPKPVRRLWLQSMTPQAIRDGFDRLRTDEQ
                     MLGLAAAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEK
                     IRKHVPRDYWEIKATFDALAGQYDAKWVDPAFKKDDDPDRRADRVWDKAKAEAIAKAV
                     LGQPGKVTEESKPSSSASPLLYDLTSLQREANGRFGFSAKTTLSLAQALYEKHKVLTY
                     PRTDSRALPEDYLPVVKDTLKMLSTEDLPGPLRELSPHAAKALKEGYVKPSKRVFDNA
                     KVSDHFAIIPTLQAPKSLSDIEAKLYDLVVKRFIAVFFPSAEYQVTTRLTQVAEHRFQ
                     TNGKVLVNPGWLAVYGKEAQDDDATLVPVQPDETVRNESVEAVGSKTRPPARYNEATL
                     LSAMEGAGKLIDDEELREAMSEKGLGTPATRAQVIEGLILEKYMLREGRDLIPTAKAF
                     QLMTLLRGLGVEDLTRPDLTGNWEYKLLEMEHGRLSRDAFMGDIAQMAERIVKKAKEY
                     DRDTIPGDYATLRTPCPNCGGIVKENYRRYTCQGPKGDGEGSCEFSITKIPAGRAFET
                     EEVEQFLRDKKIGPLEGFRSKAGWPFTAELRLVFDEEIRNWKLEFDFGEEAKEGDGEP
                     VDFSDQESLGACPKDAGRVFEHGTSYVCEHAVGAHVTCDFKSGKIILQQPVSRENMAK
                     LLATGKTDLMENFVSNKTRRKFKAFLAYDKKEGKVVFEFEPRAAKAPPAKKVAGKKKA
                     GDEE"
BASE COUNT          386 a          983 c          963 g          564 t
ORIGIN      
        1 atgtcatcct gcgcgcagtc gcaggatcca cggtggccac gcaacgttgt cgccgaggtg
       61 gatcctgcga ctccgattcg ctccgcgcag gatgacgact tggcgctgcg cgtgatgcgg
      121 cattcccggg atggcgcggc gcgcactcga cgtgaggcac tgctgccgct tcgcgcccac
      181 caggcagggt gacggcacgg gtggcgcgct ggccacccgc cccgatcact cctcgtcgcc
      241 cgccttcttc ttgccggcca ccttcttggc cggcggtgcc ttggcggcgc gcggctcgaa
      301 ctcgaagacc accttgcctt ccttcttgtc gtaggcgagg aaggccttga acttgcggcg
      361 cgtcttgttg gagacgaagt tctccatcag gtcggtcttg ccggtggcca gcagcttggc
      421 catgttctcg cgcgagaccg gctgctgcag gatgatcttg ccgctcttga aatcgcaggt
      481 gacgtgcgcg cccaccgcgt gctcgcagac atacgacgtg ccgtgttcga acacgcggcc
      541 ggcgtccttg gggcatgcgc ccagcgactc ctggtcggag aagtccaccg gctcgccgtc
      601 gccttccttg gcctcctcgc cgaagtcgaa ctcgagcttc cagttgcgga tctcctcgtc
      661 gaacaccagc cgcagctccg cggtgaaagg ccagccggcc ttggagcgga agccctcgag
      721 cgggccgatc ttcttgtcgc gcaggaactg ctcgacctcc tcggtctcga acgcgcggcc
      781 ggccgggatc ttggtgatcg agaactcgca gctgccctcg ccgtcgccct tggggccctg
      841 gcaggtgtag cggcggtagt tctccttgac gatgccgccg cagttggggc acggcgtcct
      901 gagcgtggcg tagtcgccgg ggatcgtgtc gcggtcgtat tccttggcct tcttgacgat
      961 gcgctcggcc atctgcgcga tgtcgcccat gaacgcgtcg cgcgacaggc ggccgtgctc
     1021 catctccagc agcttgtatt cccagttgcc ggtcaggtcg ggccgcgtca ggtcctcgac
     1081 gcccaggccg cgcagcagcg tcatcagctg gaacgccttg gccgtgggga tgaggtcgcg
     1141 cccctcgcgc agcatgtact tctcgaggat caggccttcg atcacctgcg cccgcgtggc
     1201 cggcgtgccc aggcccttct cggacatcgc ctcgcgcagt tcctcgtcgt cgatcagctt
     1261 gcccgcgcct tccatcgccg acagcagcgt cgcctcgttg tagcgcgccg gcggacgcgt
     1321 cttcgagccg acggcctcca ccgattcgtt gcgcacggtc tcgtcgggct gcaccggcac
     1381 cagcgtggcg tcgtcgtcct gcgcctcctt gccgtagacg gccagccagc ccgggttcac
     1441 cagcaccttg ccgttggtct ggaagcggtg ctcggcgacc tgcgtcagtc gggtcgtgac
     1501 ctggtattcg gcgctcggga agaacacggc gatgaagcgc ttgaccacca ggtcatacag
     1561 cttggcctcg atgtcggaca gcgacttggg cgcctgcagc gtcgggatga tggcgaagtg
     1621 gtccgacacc ttggcgttgt cgaacacccg cttggagggc ttgacgtagc cttccttgag
     1681 cgccttggcc gcgtgcggcg acagctcgcg cagcgggccc ggcaggtcct cggtggacag
     1741 catcttgagc gtgtccttga ccaccggcag gtagtcctcg ggcagcgcgc gcgagtccgt
     1801 ccgcggatag gtgagcacct tgtgcttctc gtacagcgcc tgcgccagcg acagcgtggt
     1861 cttggccgag aagccgaagc ggccgttggc ctcgcgctgt aggctggtca ggtcgtacag
     1921 cagcggactg gccgagctgc tgggcttgga ttcctcggtg accttgccgg gctggcccag
     1981 gacggccttg gcgatcgcct cggccttggc cttgtcccac acgcggtcgg cgcggcggtc
     2041 ggggtcgtcg tccttcttga acgccgggtc gacccacttg gcgtcgtact ggccggccag
     2101 cgcgtcgaac gtggccttga tctcccagta gtcgcgcggc acgtgcttgc ggatcttctc
     2161 ctcgcgttcg accacgatgg acagggtggg cgtctggacc cggccgacgg tggtcaggaa
     2221 gaagccgccg tcgcgcgagt tgaacgccgt catcgcgcgc gtgccgttga tgccgaccag
     2281 ccagtcggcc tccgaccggc tgcgcgcggc ggcggccagg cccagcatct gttcatcggt
     2341 gcgcagcctg tcgaagccgt cgcggatggc ctgtggcgtc atgctctgca gccacaggcg
     2401 gcgcaccggc ttcgggctct tcttgtcgat gccggcgtac tgcacgatca ggcggaagat
     2461 cagctcgccc tcgcggcccg cgtcgcaggc gttgatgagc tcggtgacgt ccttgcgctt
     2521 gaccagcttg accacggcat tgagccgcgt cttggccttg tcgatcggcg ccagctcgaa
     2581 gtgcggcggg atcaccggca ggtgggcgaa gctccacttg ccgcgcttga cctcgtactc
     2641 ttccggcgcc ttgatctcca ccaggtggcc cacggcggag gtgacgacgt acttgtcgtt
     2701 ctcgaagtgc tcggcgtgct tctcgaactt gccggccacc ggcgtcagcg cgcgaacaat
     2761 gtcctgcgcg acgctcggct tctcggcaat gaccagggac ttgctcatcg gtgatgtgtg
     2821 atttcttggc ggcctacaat ggcccgatct cgcgcgcacg catacgcata cacgcgcgcg
     2881 cacccatgaa ttcaat
//
LOCUS       DATCRO010000595         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_513355, whole genome shotgun sequence.
ACCESSION   DATCRO010000595 DATCRO010000000
VERSION     DATCRO010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513355"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VIN75_16225"
     CDS             complement(<1..620)
                     /locus_tag="VIN75_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8975768.1"
                     /translation="MRNHPLGRTGLFVSELCLGTMTFGGSEGIWGKIGDLGQADAERL
                     VGQAIDAGINFIDTADVYSGGVSEQITGQALKNLKVPRESVVVATKVFGETGPGANAR
                     GLSRGHIADGVKSSLKRLQLEHIDLYQVHGFDPATPVEETVRALDLLVRQGHVRYVGV
                     SNWAAWQIAKAVGIAERLGLSRFESLQAYYTLAGRDLEREIVPMLK"
     gene            870..2186
                     /locus_tag="VIN75_16230"
     CDS             870..2186
                     /locus_tag="VIN75_16230"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8975769.1"
                     /translation="MHRPGFVFGAATASYQIEGAAREDGRVPSIWDTFSHTPGRTHEG
                     DTGDVACDHYHRYEQDVALLADLGFDAYRFSIAWPRVMDENGRLNPRGIDFYKRLLDQ
                     LDKRGISAWATLYHWDLPQHLQDRGGWLNRDTAHRFAEYAGQMAQAFKGRIAGWMTLN
                     EPFCSAYIGHSEGRHAPGLTDARYGVEAMHHLLLAHGLGTAALRAHDKAPVGLVANVC
                     QVTPASDSEADRVATERAFASSNVWPLDPLFLGRYPAPLRDLWPDAHAPVREGDMAVI
                     GTPLDFVGINYYFRQVIASDGGHGFVVKPLPGVERTQMGWEVFPDGLRDLLLEFRQRY
                     PGLPPVYITENGMASADTVEGGRVQDAQRARYFNSHLAAIDEAMRQGVDVRGYFAWSL
                     MDNFEWSYGYEKRFGLVHVDYATQVRTPKDSALAFRQFLQARRAAA"
     gene            2249..3490
                     /locus_tag="VIN75_16235"
     CDS             2249..3490
                     /locus_tag="VIN75_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975770.1"
                     /translation="MTNKTTRLALALALAFGALTGAHAQANRAEVLHWWTSGGESAAV
                     KELANAYRAAGGTWVDDAIAGGEQARAEAVNRMVGGKPPTAAQFNTSKQFLDLIDQGM
                     LANVDVVAAQNNWDRILPAPILNSIRIKGHFYAVPVDIHMPAWFWYSKAAFAKAGIKD
                     EPKSADEFFADLDKLKAAGVVPLAFGGQPWQEKITFYAWLAHVGGPDMYMKLYRDKDA
                     NLPATPQFKALLASFKKLHNYVDAGSPNRNWNDATAMVISGKAGVQIMGDWAKGEFAT
                     AHMTAGKEFGCFPGFGPKSPYLVAGDVFVFPKIADPAQVKSQQLLATVFTSPATQVAF
                     SNKKGSIPIRTDVDVKGLDLCAQQGVTIMKDVSRQLPTPEMLISPDLEGSLQDVITKF
                     WNTSESVDDAAKAISAALKNG"
     gene            3551..4426
                     /locus_tag="VIN75_16240"
     CDS             3551..4426
                     /locus_tag="VIN75_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8975771.1"
                     /translation="MVATVLWVYVGSVLWSMRISVSSSRTLPIGDFVGIRQYARLFDS
                     ERWMISLQNMALFGALFILGCLVIGFLLAVFIDQRVRAEGVLRTIFLYPYAMSFVATG
                     LVWQWMLNPELGLQSTVRGWGFPNFTFDWIVRQETVMLAIVVAAVWQACGLVMALMLA
                     GLRGIDESLWMAARIEGIPKWRVYLSIVLPMMGPSFGTAFVLLSVSVVKVFDVVVAMT
                     QGGPGLASEVPAKFIIDNLLVRANIGLASAASTVLLLTVLALLAPWFYARAHASRRGE
                     RVARALRASAQGANA"
     gene            4423..>5297
                     /locus_tag="VIN75_16245"
     CDS             4423..>5297
                     /locus_tag="VIN75_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8975772.1"
                     /translation="MNAVVTPVADGLGAEAVAPRPPLAAARRRLPRPARIGLYVFLAT
                     AAFAFLLPLYVMVVTSIKPMDEIRLGNLFAWPVKVTLEPWAQAWTSACTGLECGGIRV
                     GFMNSVAIVAPSTILSILLGALNGYALSFWRPRGAQAFFVVLLTGAFIPYQVMLFPLI
                     RALAAMGLFGTLPGVVLIHTLFGMPVMTLLFRNYYASVPHELFMAARIDGGGFWRIFV
                     QLMLPMSLPIVVVAVIMQVTAIWNDFLIGLVFAGHDWLPMTVQLNNIINTTTGERLYN
                     VNMAATLLTSLVPLTV"
BASE COUNT          775 a         1891 c         1776 g          855 t
ORIGIN      
        1 ctcttcaaca tcggcacgat ctcgcgctcg aggtcgcgcc cggccagcgt gtagtacgcc
       61 tgcagcgact cgaagcgcga cagccccagc cgttccgcga tgcccaccgc cttggcgatc
      121 tgccacgcgg cccagttcga cacgcccacg tagcgcacgt ggccttgccg caccagcagg
      181 tcgagcgcgc ggaccgtctc ctcgacgggc gtggccggat cgaagccgtg cacctggtac
      241 aggtcgatgt gctcgagctg caggcgcttc aggctggact tgacgccgtc ggcgatgtgg
      301 ccgcgcgaga gaccgcgcgc gttggcgccg ggccccgtct cgccgaacac cttggtcgcg
      361 acgacgacgc tctcgcgcgg gaccttcagg ttcttcagcg cctgcccggt gatctgctcc
      421 gagacgccgc ccgagtagac gtcggcggtg tcgatgaagt tgatgccggc gtcgatggcc
      481 tgccccacga ggcgctcggc gtccgcctgc ccgaggtcgc cgatcttgcc ccagatgcct
      541 tcgctgccgc caaaggtcat cgtgcccagg cacagttctg agacgaacag gccggtgcgg
      601 cccagaggat ggttgcgcat gtcgaagtgc tccatgagga aggaaaggcg gcggccagca
      661 taggggcccg ggcaaagaca tccgggtcgc ggggtcatgt ccgagcacct cggtgtttcc
      721 actgatacgc ccccctgccc tgccgtgcag aatcgcgcca cgtggaaccg gttccacgtc
      781 gcagcttgcg aacctgcgcc ccgccgcgcg caccgaggcc cgtcttgagc aatccgatcg
      841 ttcccatctc tccgccgccc ggctcggcga tgcatcgccc cggtttcgtg ttcggcgcgg
      901 ccacggcctc gtaccagatc gagggcgcgg cccgcgagga cggccgcgtg ccgtcgatct
      961 gggacacctt ctcgcacacc ccgggcagga cgcacgaggg cgacaccggc gacgtcgcct
     1021 gcgaccacta ccaccgctac gagcaggacg tcgcgctcct ggccgacctc ggcttcgacg
     1081 cctaccgctt ctcgatcgcc tggccgcgcg tgatggacga gaacggccgc ctgaacccgc
     1141 gcggcatcga cttctacaag cgcctgctcg accagttgga caagcgcggc atctccgcct
     1201 gggcgacgct gtaccactgg gacctgccgc agcacctgca ggaccgcggc ggctggctca
     1261 accgcgatac cgcgcaccgt ttcgccgagt acgccgggca gatggcccag gcgttcaagg
     1321 gccgcatcgc cggctggatg acgctcaacg agccgttctg ctccgcctac atcggccact
     1381 ccgagggccg gcatgcgccc ggcctgaccg acgcgcgcta cggcgtcgag gcgatgcacc
     1441 acctgctgct ggcgcacggc ctgggcacgg cggcgctgcg cgcgcacgac aaggcgcccg
     1501 tcggcctggt cgccaacgtc tgccaggtca cgccggccag cgactccgag gccgaccgcg
     1561 tcgccaccga gcgcgcgttc gccagctcca acgtgtggcc gctcgacccg ctgttcctgg
     1621 gccgctatcc ggccccgctg cgggacctgt ggcccgacgc gcacgcgccg gtgcgcgagg
     1681 gcgacatggc cgtcatcggc acgccgctcg acttcgtcgg catcaactac tacttccgcc
     1741 aggtgatcgc gagcgacggc ggccacggct tcgtggtcaa gccattgccc ggcgtcgagc
     1801 gcacgcagat gggctgggag gtgttccccg acggcctgcg cgacctgctg ctcgagttca
     1861 ggcagcgcta cccgggcctg ccgcccgtct acatcaccga gaacggcatg gccagcgccg
     1921 acacggtcga gggcggccgc gtgcaggacg cgcagcgcgc tcgctacttc aacagccacc
     1981 tcgcggccat cgacgaggcg atgcgccagg gcgtggacgt gcgcggatat ttcgcgtggt
     2041 ccctgatgga caacttcgag tggagctacg gctacgagaa gcgcttcggc ctcgtccacg
     2101 tcgactacgc gacgcaggtg cgcacgccca aggacagcgc gctcgcgttc aggcagttcc
     2161 tgcaggcccg gcgcgccgcg gcctgagcct cttttcgccg aagcggccgg tgacgacgcc
     2221 gcatgaacac ccagcaagga gacgacgcat gaccaacaag accacccgcc tggccctcgc
     2281 gctggcgctc gcgttcggcg cgctcaccgg cgcccacgcc caggccaacc gcgccgaggt
     2341 gctgcactgg tggacgtccg gcggcgagtc cgcggccgtc aaggaactgg ccaacgcgta
     2401 tcgcgcggcg ggcggcacct gggtcgacga cgcgatcgcc ggcggcgagc aggcgcgcgc
     2461 cgaggccgtc aaccgcatgg tcggcggcaa gccgcccacc gcggcgcagt tcaacacctc
     2521 caagcagttc ctcgacctga tcgaccaggg catgctggcc aacgtcgacg tggtcgccgc
     2581 gcagaacaac tgggacagga tcctgcccgc gccgatcctg aactcgatca ggatcaaggg
     2641 ccacttctac gcggtgccgg tggacatcca catgccggcc tggttctggt attcgaaggc
     2701 ggcgttcgcc aaggccggca tcaaggacga gccgaagtcg gccgacgagt tcttcgccga
     2761 cctggacaag ctcaaggccg ccggcgtcgt gccgctggcc ttcgggggcc agccgtggca
     2821 ggagaagatc acgttctacg cgtggctcgc gcacgtcggc ggtccggaca tgtacatgaa
     2881 gctgtaccgc gacaaggacg ccaacctgcc cgccacgccg cagttcaagg cgctgctggc
     2941 ctcgttcaag aagctgcaca actacgtcga cgccggctcg cccaaccgca actggaacga
     3001 cgccaccgcg atggtcatct ccggcaaggc cggcgtgcag atcatgggcg actgggccaa
     3061 gggcgagttc gccacggcgc acatgaccgc gggcaaggag ttcggctgct tcccgggctt
     3121 cggcccgaag tcgccgtacc tcgtcgcggg cgacgtgttc gtgttcccga agatcgccga
     3181 cccggcgcag gtcaagtcgc agcagctgct ggccaccgtc ttcacgtcgc cggccacgca
     3241 ggtcgcgttc tccaacaaga agggctcgat cccgatccgc acggacgtcg acgtcaaggg
     3301 cctggatctg tgcgcgcagc agggcgtgac gatcatgaag gacgtctcgc gccagctgcc
     3361 gacgcccgag atgctgatct cgccggatct cgaaggctcg ctgcaggacg tcatcacgaa
     3421 gttctggaac acgagcgagt cggtcgatga cgcggccaag gccatctccg ccgcgctgaa
     3481 gaacggctaa gtgaagtccg cttcccgccg ccctctccgc cacctcgtcg cctgggccgc
     3541 gctgctgccc atggtggcga cggtgctgtg ggtctacgtc ggcagcgtgc tgtggtcgat
     3601 gcggatctcg gtcagcagct cgcgcacgct gccgatcggc gacttcgtcg gcatcaggca
     3661 gtacgcgcgc ctgttcgaca gcgagcgctg gatgatctcg ttgcagaaca tggcgctgtt
     3721 cggcgcgctg ttcatcctcg gctgcctggt catcggcttc ctgctggccg tgttcatcga
     3781 ccagcgggtg cgcgccgagg gcgtgctgcg cacgatcttc ctgtacccgt acgcgatgtc
     3841 gttcgtggcc accggcctgg tctggcagtg gatgctcaac cccgagctgg ggctgcagtc
     3901 gacggtgcgc ggctggggct ttccgaactt cacgttcgac tggatcgtgc gccaggagac
     3961 ggtgatgctc gcgatcgtcg tggccgccgt ctggcaggcc tgcggcctcg tgatggcgct
     4021 gatgctggcc gggctgcgcg gcatcgacga gagcctgtgg atggccgcgc gcatcgaggg
     4081 catcccgaag tggcgtgtct acctgagcat cgtgctgccg atgatggggc cgtcgttcgg
     4141 caccgcgttc gtgctgctgt cggtgagcgt cgtcaaggtg ttcgacgtcg tcgtcgcgat
     4201 gacgcagggc gggccgggcc tggccagcga ggtgccggcc aagttcatca tcgacaacct
     4261 gctggtgcgc gccaacatcg gcctggcctc cgccgcgtcg accgtgctgc tgctgacggt
     4321 gctggcgctg ctcgcgccgt ggttctatgc ccgcgcgcac gcgtcacgcc gcggcgagcg
     4381 cgtcgcgcgc gcgctgcgtg cgagcgcgca gggagcgaac gcatgaacgc cgtcgtcacg
     4441 cccgtcgccg acggcctggg cgccgaggcg gtcgcccccc gcccgccgct ggccgccgcc
     4501 cgccgccgcc tcccgcgccc tgcgcgcatc ggcctgtacg tgttcctcgc caccgcggcg
     4561 ttcgcgttcc tgttgccgct gtacgtgatg gtcgtcacgt cgatcaagcc gatggatgag
     4621 atccgcctgg gcaacctgtt cgcgtggccc gtcaaggtga cgctggagcc gtgggcgcag
     4681 gcgtggacct cggcctgcac gggcctcgaa tgcggcggca tccgcgtcgg cttcatgaac
     4741 tcggtggcca tcgtggcgcc cagcaccatc ctgtcgatcc tgctgggcgc gctcaacggc
     4801 tacgcgctgt cgttctggcg gccgcgcggc gcgcaggcgt tcttcgtcgt gctgctgacc
     4861 ggcgccttca tcccctacca ggtgatgctg ttcccgctga tccgcgcgct cgccgcgatg
     4921 gggctgttcg gcacgctgcc cggcgtggtg ctgatccaca cgctgttcgg catgccggtg
     4981 atgacgctgc tgttccgcaa ctactacgcc agcgtgccgc acgagctgtt catggccgcg
     5041 cgcatcgacg gcgggggctt ctggcgcatc ttcgtgcagc tgatgctgcc gatgtccctg
     5101 cccatcgtcg tggtggccgt gatcatgcag gtcaccgcga tctggaacga cttcctgatc
     5161 ggcctcgtgt tcgccggcca cgactggctg ccgatgaccg tgcagctgaa caacatcatc
     5221 aacaccacca ccggcgagcg gctctacaac gtcaacatgg cggccacgct gctgacgtcg
     5281 ctggtgccgc tgaccgt
//
LOCUS       DATCRO010000596         9633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_861876, whole genome shotgun sequence.
ACCESSION   DATCRO010000596 DATCRO010000000
VERSION     DATCRO010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861876"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..2459)
                     /locus_tag="VIN75_16250"
     CDS             complement(291..2459)
                     /locus_tag="VIN75_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY8975773.1"
                     /translation="MTHSPIQIDGRWYVLATGAPAEEKPQTLKRDALFGCFDRHGDIV
                     PWEGAEQGLYAEDTRYLSRLELRLDGARPLYLNTAVRDDGSVLAVELMNPDLQRGDRI
                     AVPKGSVHLLRRKLLRDGTCHEQVRLANHGLEPVELTLELSFDADFADLFEVRGTPRQ
                     RRGVRLPPAVEDGALVFAYDGVDGRARITRVRFDDPPTVIEPGFVSYRVRLAPAESLA
                     LQWEIVPRRPGVVPTSSLTYDDARRLHEERLVVGSNGCRLASSNPMLDRWLQRSASDL
                     DMLTTALPTGPYPYAGVPWYCTTFGRDGLITAHQCLWLRPELARGTLAFLASTQATER
                     DPASDAEPGKILHEARASEMALTREVPFARYYGSVDATPLFVMLAAAYWRRSADDEFM
                     RGLWPHVVAAMRWVEEYGDRDGDGFLEYARESGDGLIQQGWKDSHDSVFHSDGRMAAP
                     PIALCEVQGYAYAARIGAAELAQAMGEDAAASRWRAQAQALRAQFLQSFWSEGLGLYA
                     LALDGGKRRCDVASSNAGHALWTGIAAPEHAARIAERFLEPDMFSGWGVRTLGTGEVR
                     YNPMSYHNGSIWPHDNALICEGLARYGRVDAALALLGAAFDTSVHFDDARLPELFCGF
                     PRRAGEGPTRYPVACSPQAWAAGAVFGMLAGCLGLTIDARERRVQLRSPRLPAFVDRL
                     RVERLAIAGGNVDLLLQRYKDSVGVEVTRREGEVEVSVVV"
     gene            complement(2456..3538)
                     /locus_tag="VIN75_16255"
     CDS             complement(2456..3538)
                     /locus_tag="VIN75_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8975774.1"
                     /translation="MKIAQVAPLFESVPPRGYGGTERVVSYLTEALVDLGHDVTLYAS
                     GDSRTRARLVSIVERSLRLDPRQPDWLMWHTLMLDRVFTDAPDFDVMHFHVDFLHYPL
                     VRRCPTPCLTTLHGRLDLPDLAPLHRHFREQGLVSISNSQRVPLAGSRMLATVHHGLP
                     EELYRFHPCAGDYFAFLGRISPEKRVDRAIRIAQAAGVPLRIAAKIDRADQAYFEQEI
                     RPLLAHPLIHFEGELDERGKDEFLGNARALLFPIDWPEPFGLVMIEALACGTPVIAWR
                     CGSVPEVLDQGVTGWIVSDLDQAIAAARRIDRIDRASCRRAFEQRFTARRMAQQYVEV
                     YAALSAAGRGECDDDEAESESLPEPR"
     gene            3926..5092
                     /locus_tag="VIN75_16260"
     CDS             3926..5092
                     /locus_tag="VIN75_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY8975775.1"
                     /translation="MNNAHIIGWGHTPFGKLDAIDLEQLIRDAALPAVASAGLQLSDI
                     DGIFVGHFNGGFVQQDFSGALAGVAIPEFRHVPAMRVENACATGSAAIWAALDAVGSG
                     RVGHALVIGMEKMNTLPSAQIGDTLLKCSYVKEEADTAGGFAGVFGDIAGVYFERFGD
                     QSDALAAISAKNHENGVRNPFAHMKRDLGYDFCRHPSDKNPFVAGPLKRSDCSLVSDG
                     AAALVISREPVAGALVRPVRWRSRTQVNEYLPLSRRDPTKFEGAALAWQRGLETAGVT
                     LADLHFVETHDCFTIAELLEYEAMGLRPHGQGARAILDGDTRRDGRLPVNPSGGLKSR
                     GHPIGATGVSQHVMAAMQLTGSAGDMQLPRAELGAVFNMGGAAVANYLSVLEAA"
     gene            5089..6711
                     /locus_tag="VIN75_16265"
     CDS             5089..6711
                     /locus_tag="VIN75_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY8975776.1"
                     /translation="MTHPAQVCNLSQLLRQTAALHPDRPGLIQGDRQWTWREIDDRVD
                     AMVAALRKLGIRQGDRLLVQSRNTLAMFESCWVAFRLGAVWVPVNFRLTPPEVAYLGA
                     SSGAVAMLVDDGFEAHVAAVREASPALRVIVPMGAGAPGQANWESLVAAHRGAARHEA
                     TVGADDPLWFFYTSGTTGRPKAGMLTHGQMAFVVTNHLADLIPGTTEHDVSIAVAPLS
                     HGAGIHALLNVARGAATVLLPSEKLEPAVFWQLVERHRVSNLFTVPTIVKMLVEHPAV
                     DAHDHSSLRYMIYAGAPMYRADQQRALRTLGPVLVQYFGLGEVTGCITVLPPSMHAAD
                     DDAPEANVGSCGRPRTGMEVAILDADCNPLPVGEVGEICCRGPGVFAGYHDNNEATAK
                     ALRGGWFHTGDLGRVDARGLLYITGRESDMYISGGSNVYPREVEEVLLTHPAVAEVAV
                     LGVPDEKWGEVGVAVVVAREGHDCDAAALLAHLEGRCARYRWPRHVFFWSALPKSAYG
                     KITKKDVREQLFASGQLWESTSLGKTRESTPA"
     gene            6805..7812
                     /locus_tag="VIN75_16270"
     CDS             6805..7812
                     /locus_tag="VIN75_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8975777.1"
                     /translation="MQLTRRQILAGAATSLALPSMGAFAQKAEFTYKYANNLPVSHPM
                     NLRAKEMADAIRAETKGRVDIQIYPNNQLGSDSDMLSQLRSGGIEFFTLSGLILGQLV
                     PASQISGIGFAFPDYATVWKAMDGELGAWIRAQIAKKDLVAMDRIWDNGFRQTTSSTK
                     PINTPEDFKGFKIRVPVSPLWTSMFKAFDASPASINFAEVYSSLQTKVVEGQENPLAI
                     ISTAKLYEVQKYCSLTNHMWDGFWFLANKRAWDKLPADLQAIVAKHINAAGVKERADV
                     AELNANLQKDLASKGMVFNSTNASLFRDHLRKANFYSDWKSKFGEEGWAVLERSVGKL
                     S"
     gene            7877..>9633
                     /locus_tag="VIN75_16275"
     CDS             7877..>9633
                     /locus_tag="VIN75_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8975778.1"
                     /translation="MKQVVRLDDLAVDPTRDALGHVLPEAANGPIVTQAARPRGVLGR
                     IDWALGWLVEVPAAALVVADIAVLLAGVVARFVFHNPLVWSDELASILFLWLAMFGSV
                     IALRRAEHMRMTAVVARLSPGGRALMEAVATCACLAFLAMVLGPAWEHARDEADITTP
                     ALEISNLWREIAMPIGIGLMGIFAMLRLLREASMKHMVAALVLVAGICAAFWFGRGAF
                     EDLGRLNLLVFFVGVAAGSVLAGIPIAFAFGLGTFGYLALATGTPMPVMVGRMDEGMS
                     HLILLAVPLFVFLGQLLEATGMARAMVAFLAGLLGHVRGGLQYVLVGAMYLVSGISGA
                     KAADMAAVAPALFPEMKARGADEGELVALLAATGAQTETVPPSLVLITIGSVTGVSIA
                     ALFTGGLLPALVLGLILCSVVGWRSRGEDLSRVPRTPWKQNLRLFAIALPALALPFLI
                     RAAVVEGVATATEVSTIGIAYALVVGVALYRQLRWRALGRMLVGTAALSGAILLIIGT
                     ATAMAWGLTQSGFSGWLASTMAGLPGGAPMFLAVSIVAFVILGSVLEGIPAMVLFGPL
                     LFPIARQLGIHDVHYAMVAT"
BASE COUNT         1450 a         3367 c         3375 g         1441 t
ORIGIN      
        1 ccgccatgca taagctcgcg caattgtgct tcggcgtcgt tcacacaggc cagccctacc
       61 gcgctgattg gcctgtcaag gcttgacgcc cgagacggta tctacgaggc gccgaaccgg
      121 tcagcggtgc gtggcaactc ctggccgaac tgcgacctca gacatctttc ccatctggaa
      181 cgccggaggt cccggttatc agtgctcgat catgtcgccg tccggatcga ggatccgcgt
      241 cacctgccgt tccgaggccg caggcagccg caccgttccg gaccttccga tcaaacgacc
      301 accgagactt cgacctcgcc ttcgcgtcgg gtgacctcga cgccgacgct gtccttgtag
      361 cgctgcagca gcaggtcgac gttacctccg gcgatggcca ggcgctcgac cctcaatcga
      421 tcgacgaagg cgggcaggcg tggcgagcgc agttggacgc ggcgctcacg cgcgtcgatc
      481 gtcagtccca ggcaacccgc gagcatgccg aaaaccgcgc cggcggccca ggcctgcggc
      541 gagcacgcca ccggatagcg cgtcggccct tcgcccgcgc ggcgcgggaa gccgcagaac
      601 agctccggca ggcgcgcgtc gtcgaaatgc acgctggtgt cgaaggcggc gccaagcagc
      661 gcgagcgccg cgtcgacccg gccgtagcgc gcgaggccct cgcaaatgag cgcattgtcg
      721 tgcggccaga tcgaaccgtt gtggtacgac atcgggttgt atcggacctc gcccgtgccg
      781 agcgtgcgca ccccccagcc ggagaacatg tcgggctcca ggaaccgctc tgcgatgcgc
      841 gcagcgtgct ccggcgcggc gatgccggtc cacagcgcgt ggcccgcatt cgacgacgcg
      901 acatcgcacc gccgcttgcc gccgtcaagc gccaatgcgt acagaccgag accctcgctc
      961 cagaaagact gcaggaactg agcccgcagc gcctgggcct gggcccgcca gcgcgacgcg
     1021 gccgcgtcct cgcccatcgc ctgcgccagc tctgccgcgc cgatccgcgc cgcgtaggcg
     1081 tacccctgaa cctcgcacag cgcgatcggg ggcgcggcca tgcggccgtc cgaatggaag
     1141 accgaatcgt gcgaatcctt ccagccttgc tggatcaggc cgtcgccgct ctcgcgcgcg
     1201 tactcgagga agccgtcgcc gtcgcggtcg ccgtactctt cgacccatcg catcgcggcg
     1261 acgacatgcg gccagaggcc tcgcatgaac tcgtcgtccg cactgcgccg ccagtacgcg
     1321 gccgcgagca tgacgaacag gggggtcgcg tccaccgagc cgtagtagcg cgcgaacggc
     1381 acctcgcgcg tgagcgccat ctcgctcgcg cgtgcctcgt gcaggatctt ccccggctcg
     1441 gcgtcgctgg ccgggtcgcg ctccgtcgcc tgtgtcgagg cgaggaaggc gagcgtgcca
     1501 cgcgccagct ccggccgcag ccacaggcac tggtgcgcgg tgatgagacc gtcgcggccg
     1561 aaagtcgtgc aataccaggg cacgcccgca tacgggtagg ggccggtggg cagcgcggtg
     1621 gtcagcatgt cgagatcgga cgccgagcgc tgcagccacc ggtccagcat ggggttagaa
     1681 ctcgcgagcc ggcagccatt gcttccaacg accaggcgtt cctcgtggag gcggcgcgcg
     1741 tcgtcgtagg tcagcgagga ggtcggcacg acacccgggc gtcgcgggac gatctcccac
     1801 tgcagcgcca gggactcggc cggcgcgagg cgcacgcggt agctgacgaa gcctggctcg
     1861 atcacggtgg gcggatcgtc gaagcgcacc cgggtgatcc gggcgcggcc gtccacgccg
     1921 tcgtaggcga agaccagggc accgtcctcg accgcaggcg gcaggcggac gccgcggcgc
     1981 tggcgcggcg tcccgcgcac ctcgaagagg tcggcgaagt cggcgtcgaa gctcagctcc
     2041 agcgtcaatt cgaccggctc gagcccgtgg ttcgcgaggc gcacctgctc atggcaggtg
     2101 ccgtcgcgca ggagtttgcg gcgcaacagg tggacgctgc ccttgggcac ggcgatgcgg
     2161 tcgccgcgct ggaggtcagg gttcatcagt tcgaccgcca gcacgctgcc gtcgtcgcgc
     2221 accgcggtgt tcaggtagag cggacgcgcg ccgtccagac gcagctcgag gcgcgagagg
     2281 taacgggtgt cctcggcgta gaggccctgc tcggcgccct cccagggcac gatgtcgccg
     2341 tgccggtcga aacagccgaa cagcgcgtct cgtttgagtg tctgcggctt ttcctcggct
     2401 ggcgcgccag tggccagaac gtaccagcga ccgtcgatct ggatggggct gtgggtcatc
     2461 gcggttcggg caagctttcg ctctccgcct cgtcatcgtc gcattcgccg cggcccgcgg
     2521 cgctgagggc cgcatagacc tcgacatact gttgggccat gcgccgtgcc gtgaagcgct
     2581 gttcgaaagc ccgccggcag ctcgcgcggt cgatccggtc gatgcggcgt gccgcggcga
     2641 tggcctggtc caggtcgctc acgatccatc cggtgacacc ctggtcgaga acctcgggca
     2701 ccgagccgca gcgccaggcg atgaccggcg tgccgcaggc cagcgcctcg atcatcacga
     2761 ggccgaacgg ttcgggccag tctatcggga acaggagggc gcgggcattg ccgaggaatt
     2821 catccttgcc acgctcgtcc agctcgccct cgaaatggat gagcggatgg gcgagcagcg
     2881 gccggatctc ctgctcgaag taagcctgat cggcgcggtc gatcttggcc gcgatgcgta
     2941 gcggcacccc ggccgcctgc gcgatgcgga tggcgcggtc gacgcgcttt tccggcgaga
     3001 tgcggcccag aaatgcgaag tagtcaccgg cgcacggatg gaagcggtag agctcttcgg
     3061 gcaacccgtg gtgcacggtg gccagcatgc gcgagccggc gagcggtacg cgctggctgt
     3121 tcgagatgct gacgagacct tgctcgcgga agtggcggtg cagcggggcc aggtcgggca
     3181 ggtccagccg gccatgaagc gtggtcaggc agggggtggg gcagcgccgc accaggggat
     3241 agtgcaggaa gtccacatgg aagtgcatga cgtcgaagtc gggcgcgtcc gtgaacacgc
     3301 ggtcgagcat cagcgtatgc cacatgagcc agtccggctg gcgcggatcg aggcgcaggc
     3361 tgcgctcgac gatcgaaacc agccgcgcgc gggttcgtga atcgccgctg gcatacagcg
     3421 tcacgtcgtg cccgaggtcg accagggcct cggtcaggta ggagacgacg cgctccgtcc
     3481 ccccgtagcc gcggggcggg acgctttcga acaatggcgc gacctgggcg atcttcatga
     3541 gacgatcctt tcggaacaac acccaggcgc ggcaaattct gtgcgcgacg gggcaggccg
     3601 aaccccggcg ggcgacacgg atggcgtacc aggccctcat gccgaccctg ctcgacgcaa
     3661 acagcatccg gcgagcgcgc tcctctcctt gccagtgggt gtcgcggcat gcggcggcca
     3721 atcgcgacct caccggaacc tgagcgcccc gtccaggatg tgaaccggtt tctcggatgc
     3781 tgcgccgcag caagcatgtc gccgccaggg caccctgcgc agaattcgtc caccaggcgg
     3841 cgagaagcgg cgcaccgggt tcaccatccg aaccgagccg aacgacccgc gacgaattca
     3901 ggacaggcag caggacagga cacccatgaa caacgcacac atcatcggct ggggccacac
     3961 gcccttcggc aagctcgacg ccatcgacct ggagcagctg atccgcgacg ccgcgctgcc
     4021 cgcggtggcc agcgccggcc tgcagctgtc ggacatcgac ggcatcttcg tcggccactt
     4081 caacggcggc ttcgtgcagc aggacttcag cggcgcgctg gccggcgtcg cgatccccga
     4141 gttccgccac gtgcccgcga tgcgcgtcga gaacgcgtgc gcgaccggct cggccgcgat
     4201 ctgggccgcg ctcgatgccg tcggctcggg ccgcgtcggg cacgcgctgg tgatcggcat
     4261 ggagaagatg aacacgctgc ccagcgcgca gatcggcgac acgctgctca agtgctcgta
     4321 cgtcaaggag gaggccgaca ccgccggcgg cttcgcgggc gtgttcggcg acatcgccgg
     4381 cgtctacttc gagcgcttcg gcgaccagag cgacgcgctg gccgcgatct ccgccaagaa
     4441 ccacgagaac ggcgtgcgca acccgttcgc gcacatgaag cgcgacctcg gctacgactt
     4501 ctgccgccat ccgtccgaca agaacccgtt cgtcgccggc ccgctcaagc gctcggactg
     4561 ctcgctggtg tccgacggcg ccgccgcgct ggtgatctcg cgcgagccgg tcgcgggcgc
     4621 gctcgtgcgt cccgtgcgct ggcgctcgcg cacgcaggtc aacgagtact tgccgctgtc
     4681 gcgccgcgac ccgaccaagt tcgagggcgc cgcgctcgcc tggcagcgcg ggttggagac
     4741 cgccggcgtc acgctggccg acctgcactt cgtcgagacg cacgactgct tcacgatcgc
     4801 cgagctgctg gagtacgagg cgatgggcct gcgtccgcac gggcagggcg cgcgcgccat
     4861 cctcgacggc gacacgcgcc gcgacggccg gctgccggtc aatccgtcgg gcggcctgaa
     4921 gtcgcgcggg catccgatcg gcgcgaccgg tgtgtcgcag cacgtgatgg cggcgatgca
     4981 gctgaccggt tcggccggcg acatgcagct gccgcgcgcc gagctcggcg ccgtgttcaa
     5041 catgggcggc gcggcggtcg cgaactacct cagcgtgctg gaggccgcgt gacccatccc
     5101 gcgcaggtct gcaacctctc gcagctgctg cgccagaccg cggcgctgca tccggaccgc
     5161 ccgggcctga tccagggcga ccggcaatgg acgtggcgcg agatcgacga ccgcgtcgac
     5221 gcgatggtcg ccgcgctgcg caagctgggc atccgtcagg gcgaccgcct gctcgtgcag
     5281 tcgcgcaaca cgctggcgat gttcgagagc tgctgggtcg cgttccgtct gggcgccgtc
     5341 tgggtgccgg tgaacttccg gctgacgccg ccggaagtcg cctacctcgg cgcgtccagc
     5401 ggcgcggtgg cgatgctggt ggacgacggg ttcgaggccc acgtggccgc ggtgcgcgag
     5461 gcctcgcccg cgctgcgcgt catcgtgccg atgggcgcgg gcgcgccggg gcaggcgaac
     5521 tgggaatcgc tggtcgcggc gcatcgcggc gcggcgcgtc acgaggccac ggtcggcgcc
     5581 gacgatccgc tgtggttctt ctacacgtcg gggacgacgg ggcggcccaa ggccggcatg
     5641 ctgacgcacg gccagatggc cttcgtcgtc accaaccacc tggccgacct gatcccgggc
     5701 accaccgagc acgacgtgtc gatcgcggtg gcgccgctgt cgcacggcgc gggcatccac
     5761 gcgctgctga acgtggcgcg cggcgcggcc acggtgctgc tgccgtccga gaagctcgag
     5821 ccggcggtct tctggcagct cgtcgagcgc catcgcgtca gcaacctgtt cacggtgccc
     5881 acgatcgtca agatgctggt cgagcacccg gcggtcgacg cgcacgacca ctcgtcgctg
     5941 cgctacatga tctacgccgg cgcgccgatg taccgcgccg accagcagcg cgcgctgcgc
     6001 acgctgggcc cggtgctggt gcagtatttc ggcctgggcg aggtgacggg ctgcatcacc
     6061 gtgctgccgc cgtccatgca cgcggccgac gacgacgcgc ccgaggcgaa cgtgggctcc
     6121 tgcggccggc cgcgcacggg catggaggtc gccatcctcg acgcggactg caacccgctg
     6181 cccgtgggcg aggtgggcga gatctgctgc cgcggccccg gcgtgttcgc gggctaccac
     6241 gacaacaacg aggccaccgc caaggcgctg cgcggcggct ggttccacac cggcgacctc
     6301 gggcgtgtcg acgcgcgcgg cctgctgtac atcaccggcc gcgagtccga catgtacatc
     6361 tccggcggat cgaacgtcta cccgcgcgag gtcgaggagg tgctgctgac gcacccggcc
     6421 gtcgccgagg tggccgtgct cggcgtgccc gacgagaagt ggggcgaggt cggcgtggcg
     6481 gtcgtcgtcg cacgcgaggg ccacgactgc gacgccgccg cgctgctggc gcatctggag
     6541 ggccgctgcg cgcgctaccg ctggccgcgc cacgtgttct tctggagcgc actgcccaag
     6601 tcggcctacg gcaagatcac caagaaggac gtgcgtgaac aactgttcgc aagcggccaa
     6661 ttgtgggaat ccacatcgtt ggggaagacg agggaatcca cgcccgcctg agccatcaca
     6721 atcatcggta ccgccgatgc gcgacggccc cgggggtgcc gcgcgagtcc tgccagatca
     6781 cacagccaac aacggagaca cgacatgcaa ctcacccgcc gccagatcct cgccggcgcc
     6841 gccaccagcc tcgcgctgcc ctcgatgggc gcgttcgcgc agaaggccga gttcacgtac
     6901 aagtacgcga acaacctgcc ggtgtcgcac ccgatgaacc tgcgcgccaa ggagatggcc
     6961 gacgccatcc gcgccgagac caagggccgc gtcgacatcc agatctatcc gaacaaccag
     7021 ctcggctccg actccgacat gctgagccag ctgcgctcgg gcggcatcga gttcttcacg
     7081 ctgtccggcc tgatcctggg ccagctggtg ccggcctcgc agatcagcgg catcggcttc
     7141 gcgttccccg actacgccac cgtgtggaag gcgatggacg gcgaactcgg cgcgtggatc
     7201 cgcgcgcaga tcgccaagaa ggatctcgtc gcgatggacc ggatctggga caacggcttc
     7261 cgccagacca cctcgtccac caagccgatc aacacgccgg aagacttcaa gggcttcaag
     7321 atccgcgtgc ccgtgtcgcc gctgtggacg tcgatgttca aggccttcga cgcatcgccg
     7381 gcctcgatca acttcgccga ggtgtactcg tcgctgcaga ccaaggtggt cgaaggccag
     7441 gagaacccgc tggccatcat ctccacggcc aagctctacg aagtgcagaa gtactgctcg
     7501 ctgaccaacc acatgtggga cggcttctgg ttcctggcca acaagagggc gtgggacaag
     7561 ctgccggccg acctgcaggc catcgtcgcc aagcacatca acgcggccgg cgtgaaggag
     7621 cgcgcggacg tcgccgagct caacgccaac ctgcagaagg acctcgcgtc caagggcatg
     7681 gtcttcaact cgaccaacgc cagcctgttc cgcgaccacc tgcgcaaggc caacttctac
     7741 agcgactgga agagcaagtt cggcgaggaa ggctgggccg tgctcgagcg cagcgtcggc
     7801 aagctgtcct gagaagccgg cgcctcgttg aaccagcccg cccggcacac gtgcgccggg
     7861 cgtgggagat ccgaacatga aacaagtcgt ccgactcgac gacctcgcgg tcgaccccac
     7921 ccgcgacgcg ctcggccacg tgctgcccga ggccgccaac gggcccatcg tgacgcaggc
     7981 cgcgcgtccg cgcggcgtgc ttggccgcat cgactgggcg ctcggctggc tggtggaagt
     8041 gcccgctgcc gcgctggtgg tggccgacat cgccgtgctg ctggccggtg tcgtggcgcg
     8101 cttcgtcttc cacaacccgc tcgtctggtc ggacgaactc gcctccatcc tgttcctgtg
     8161 gctggcgatg ttcggctcgg tcatcgcgct gcggcgcgcc gagcacatgc gcatgactgc
     8221 cgtcgtcgcg cgcctgtcgc ccggcggccg cgcgctgatg gaggccgtgg ccacctgcgc
     8281 gtgcctcgcg ttcctggcga tggtgctcgg gcccgcgtgg gagcacgcgc gcgacgaggc
     8341 cgacatcacg acgcccgcgc tcgagatctc gaacctgtgg cgcgagatcg ccatgccgat
     8401 cggcatcggc ctgatgggca tcttcgcgat gctgcggctg ctgcgcgagg cgagcatgaa
     8461 gcacatggtg gccgcgctcg tgctggtggc gggcatctgc gcggcgttct ggttcgggcg
     8521 cggcgcgttc gaggacctcg gacggctgaa cctgctggtc ttcttcgtgg gcgtggccgc
     8581 gggcagcgtg ctggccggca ttcccatcgc gttcgcgttc gggctgggca ccttcggcta
     8641 cctcgcgctg gcgaccggca cgccgatgcc ggtcatggtc ggccgtatgg acgagggcat
     8701 gtcgcacctg atcctgctgg ccgtgccgct gttcgtgttc ctcggccaac tgctcgaggc
     8761 cacgggcatg gcgcgcgcga tggtcgcgtt cctggccggc ctgctgggcc acgtgcgcgg
     8821 cggcctgcaa tacgtgctgg tcggcgcgat gtacctggtg tcgggcatct ccggcgccaa
     8881 ggctgccgac atggccgccg tcgcgccggc gctgttcccc gagatgaagg cgcgcggtgc
     8941 cgacgagggc gagctcgtcg cgctgctcgc ggccaccggc gcccagaccg agacggtgcc
     9001 gcccagcctg gtgctgatca ccatcggctc ggtgacgggc gtgtcgatcg ccgcgctgtt
     9061 caccggcggc ctgctgccgg cgctggtgct gggcctgatc ctgtgctcgg tcgtgggttg
     9121 gcgcagccgc ggcgaggacc tttcccgcgt acccaggacc ccgtggaagc agaacctcag
     9181 gctgttcgcg atcgcgctgc ccgcgctggc gctgccgttc ctgatccgcg ccgcggtggt
     9241 cgagggcgtg gccacggcca ccgaggtgtc gaccatcggc atcgcctatg cgctggtcgt
     9301 cggcgtcgcg ctgtaccggc agctgcgctg gcgcgcgctc ggcaggatgc tggtgggcac
     9361 ggcggcgctg tcgggcgcca tcctgctgat catcggcacc gccaccgcga tggcctgggg
     9421 cctgacgcag tcgggcttct cgggctggct cgccagcacg atggccgggc tgccgggcgg
     9481 cgcgccgatg ttcctggccg tgtccatcgt cgcgttcgtc atcctcggct cggtgctgga
     9541 aggcatcccg gcgatggtgc tgttcggccc gctgctgttc ccgatcgcac gccagttggg
     9601 catccacgac gtgcactacg cgatggtcgc gac
//
LOCUS       DATCRO010000597         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_434184, whole genome shotgun sequence.
ACCESSION   DATCRO010000597 DATCRO010000000
VERSION     DATCRO010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434184"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIN75_16280"
     CDS             complement(<1..396)
                     /locus_tag="VIN75_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY8975779.1"
                     /translation="MTVPFATFSFAAGTRRGATALVIAATALLAACGGGGSGSDGRSS
                     GASGGNNAGSAQPTNTFAGTVTFQGAPLAGVTVIAFNTNTNSTFATTTTDAAGHYGFA
                     GLGISCNCTINYQFWARKDGYSFTPAIGGN"
     gene            complement(718..1011)
                     /locus_tag="VIN75_16285"
     CDS             complement(718..1011)
                     /locus_tag="VIN75_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975780.1"
                     /translation="MDDRTTDHLTRRTTLRRTAALGLGAAVAWLRTTPAGAQARLAKD
                     AVKYVDQGDVPGKDCDDCMHYIAPAKAGGPATCRLVEGAISPHGHCIAFTPKG"
     gene            complement(1076..1591)
                     /locus_tag="VIN75_16290"
     CDS             complement(1076..1591)
                     /locus_tag="VIN75_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEY8975781.1"
                     /translation="MSAVPPAVTAPPLRDPADVRSKTAATWIALVGGSLGLHHFYLRG
                     WRSALGWLYPIPTLVGLAGVVRMRNLGVDDHLAWALIPWLGLTITAAMLCAIVYGLTT
                     DAKWARQHAGDPTRPVVQATREQDEDSPPPGLVHTGWGPVLGVILALMIGGGVLMGTI
                     AFTVERLFYIR"
     gene            complement(1588..2160)
                     /locus_tag="VIN75_16295"
     CDS             complement(1588..2160)
                     /locus_tag="VIN75_16295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8975782.1"
                     /translation="MSNATASDNASLLLLRDSTAADVAACAAIYREAVRNGTGTFELD
                     EPDDAEMGRRRDAILAQGLPWLVAERGGRVLGYAYANQFRPRRAYRFCVEDSIYLDPA
                     ARGQGVGRLLLAELIGRCERLGVRQMLAVIGDAENHGSISVHRSCGFEPSGVFKAAGW
                     KFERWLDVVMMQRRLGSGADVAPVEREDLR"
     gene            2416..2685
                     /gene="rpsP"
                     /locus_tag="VIN75_16300"
     CDS             2416..2685
                     /gene="rpsP"
                     /locus_tag="VIN75_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8975783.1"
                     /translation="MVVIRLARGGAKKRPFYNIVVADSRERRDGRYLERVGFYNPMAS
                     GQEEPLRVVFDRVTYWKGVGAQVSPTVARLLDQAAKKAVGAPAAA"
     gene            2733..3374
                     /gene="rimM"
                     /locus_tag="VIN75_16305"
     CDS             2733..3374
                     /gene="rimM"
                     /locus_tag="VIN75_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551159.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8975784.1"
                     /translation="MTAAAPQEDLTWPEDAIEVGRILGAWGIKGGIKVLPFASDPQAL
                     FTAKRWFLKPAESAKPPAAAAAPANALKPPVAKTAAQKAALAAPLPFALNVKGVRDQG
                     DAIVCTAPEIPDRDAAEAMKGVRVFVSRATFPKTDDGEFYWVDLIGLAVVNREQAALG
                     DVVGLIDTGPHCVLRVQPADPAADERLIPFVEAYVDSVDMPGRRIVVDWGLDF"
     gene            3391..4164
                     /gene="trmD"
                     /locus_tag="VIN75_16310"
     CDS             3391..4164
                     /gene="trmD"
                     /locus_tag="VIN75_16310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8975785.1"
                     /translation="MRFDVVTLFPDLFAPFLTQGITRRAYESGQVDVKLWNLRDHAEG
                     TYRRVDDRPFGGGPGMVMLAEPLERALSAVASARAGAGAPAGPVVLFTPTGRRIDQAL
                     VREWADGPGATLICGRYEGIDQRVVDRRVDIELSLGDFVLSGGEIPALALLDAIARLM
                     PGVLGDEQSHLQDSFEQGLLDCPHYSRPEILATPEGELPVPPVLLSGHHKDIARWRRE
                     QSLTVTARRRPDLIAAARAAGQLTKADEKFLANLPPNTL"
     gene            4298..>4498
                     /locus_tag="VIN75_16315"
     CDS             4298..>4498
                     /locus_tag="VIN75_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8975786.1"
                     /translation="MDLIATLEQEEIARLGKNIPAFAPGDTVIVNVNVVEGTRKRVQA
                     YEGVVIARRNRGLNSAFIVRKIS"
BASE COUNT          636 a         1623 c         1574 g          665 t
ORIGIN      
        1 gttgccgccg atggccggcg tgaacgagta gccgtccttg cgcgcccaga actggtagtt
       61 gatcgtgcag ttgcaggaga tgcccaggcc cgcgaagccg tagtggccgg cggcgtccgt
      121 ggtggtggtc gcgaaggtcg agttggtgtt ggtgttgaac gcgatcaccg tgacgccggc
      181 cagcggcgcc ccctggaacg tgacggtgcc ggcgaaggtg ttggtgggct gggccgaacc
      241 ggcgttgttg ccgccgctcg cgccgctgct gcggccgtcg ctgccactcc cgccgccgcc
      301 gcaggcggcc agcagcgcgg tggcggcgat gacgagcgcc gtggcgccgc ggcgggtgcc
      361 ggcggcgaag gagaacgtgg cgaaaggaac ggtcatcgga cgggctcgcg agcggtggat
      421 gaccgttcta cggccgccga acgcccgtgc agcagggggc gccggcgcgg cggcgtgaac
      481 aatgtcgcgc cgtcccgcgc gatcggcgca tcgagccacc agttcgagca tcgagtgtca
      541 tcctgcgcgc agtcgcagga tccacggcgg cgtgcccggt gggactccga ggagcgccaa
      601 cggtagatcc tgcgactccg cttcgctgcg cgctggcccg tcgttcgcgc cgctggccaa
      661 ttcggagcgc ccgaactgca cttcgcccgg ctcgcgcgcg agttcgcgcc gaagtagtca
      721 gcctttcggc gtgaacgcga tgcagtgtcc gtgcgggctg atcgcgccct cgacgagccg
      781 gcaggtcgcc ggcccgcccg ccttggccgg cgcgatgtag tgcatgcagt cgtcgcagtc
      841 cttgcccggc acgtcgccct ggtcgacgta cttgacggcg tccttggcga gcctggcctg
      901 cgcgccggcc ggcgtcgtcc tcaaccaggc gacggccgcg cccaggccga gggcggcggt
      961 gcggcgcagc gtggtgcggc gcgtgaggtg atcggtcgtg cggtcgtcca tggcgcgctc
     1021 cttcgatgac atgcggcttg catgtcagat cgcgggagcg gcgcggatat tcccgtcagc
     1081 ggatgtagaa caggcgttcg accgtgaacg cgatcgtgcc catcagcacg ccgccgccga
     1141 tcatcagcgc caggatcacg cccagcaccg ggccccagcc cgtgtgcacg aggccgggcg
     1201 gcggcgagtc ctcgtcctgc tcgcgcgtgg cctgcacgac cggccttgtc ggatcacccg
     1261 catgttggcg cgcccatttc gcatccgtgg tcaggccgta gacgatggcg cacagcatgg
     1321 ccgccgtgat ggtcagcccc agccacggga tcagtgccca ggccaggtgg tcgtccacgc
     1381 cgaggttgcg catgcgcacg acgccggcca ggccgacgag cgtggggatc ggatacagcc
     1441 agcccaatgc gctgcgccag ccgcgcaggt agaagtggtg cagccccagg ctgcccccga
     1501 ccagcgcgat ccaggtggcg gccgtcttgg agcggacgtc ggcggggtcg cgcagcggcg
     1561 gggcggtgac cgcgggcggg acggcgctca ccgcaggtcc tcgcgttcga cgggcgcgac
     1621 gtcggcgccg ctgcccaggc gccgctgcat catcacgacg tccagccagc gctcgaactt
     1681 ccagccggcg gccttgaaca cgccgctggg ctcgaagccg caggaccggt gcaccgagat
     1741 cgagccgtgg ttctccgcgt ccccgatgac ggccagcatc tggcgcacgc ccagccgctc
     1801 gcagcggccg atcagctcgg ccagcagcag cctgcccacg ccctggccgc gcgcggccgg
     1861 atccaggtag atcgagtcct cgacgcagaa gcggtaggcg cggcgcgggc ggaactggtt
     1921 ggcgtaggcg tagcccagca cgcggccgcc gcgctcggcg accagccagg gcagcccctg
     1981 cgcgaggatc gcgtcgcggc gcctgcccat ctcggcgtcg tcgggctcgt ccagctcgaa
     2041 ggtgccggtg ccgttgcgca ccgcctcgcg gtagatcgcg gcgcaggccg cgacgtcggc
     2101 ggccgtgctg tcgcgcagca gcaggaggga ggcgttgtcc gaggcagtgg cgttgctcat
     2161 ggtgttcagt ttataatgcc cggttttcgg cgttggctgg tgccttgccg ggagcttgcg
     2221 gactgatcct ccgttggggc tggcgccccg gctccgttcc cgccccggcg ggaaagctcg
     2281 gcaccggcgt atctaacgac gctggagagc gtagcgcctg aaaccggccc cggccggcga
     2341 cggcaacctc gtttggcctg cccttgcgtg tctccctggc gcggcaaaac acaccaaaca
     2401 tcacaggaat tcatcatggt cgtcattcga cttgcccgcg gcggcgccaa gaagcgcccg
     2461 ttctacaaca tcgtcgtcgc cgactcgcgc gagcgccgcg acggtcgcta cctcgagcgc
     2521 gtgggcttct acaacccgat ggcctcgggc caggaagaac cgctgcgcgt cgtgttcgac
     2581 cgcgtcacct actggaaggg cgtgggcgcc caggtctcgc cgaccgttgc ccgcctgctc
     2641 gaccaggccg ccaagaaggc cgtcggcgct cccgccgccg cctaaatgac ggttcgtctt
     2701 cttcgctgcg cggccggccg cgccatcgcc cgatgaccgc cgctgcgccg caagaagacc
     2761 tcacctggcc cgaagatgcg atcgaggtcg gtcgcatcct cggcgcctgg ggcatcaagg
     2821 gcggcatcaa ggtgctgccc ttcgcctccg acccgcaggc cctgttcacc gccaagcgct
     2881 ggttcctgaa acccgccgag agtgcgaagc cgcccgccgc ggccgccgcc ccggccaatg
     2941 cgctcaagcc ccccgtcgcc aagaccgctg cccagaaggc cgcgctcgcc gcgccgctgc
     3001 cgttcgcgct gaacgtcaag ggcgtgcgcg accagggcga cgcgatcgtc tgcaccgcgc
     3061 cggaaatccc cgaccgcgac gccgccgagg cgatgaaggg cgtgcgcgtg ttcgtctcgc
     3121 gcgccacctt tcccaagacc gacgacggcg agttctactg ggtcgacctg atcggcctgg
     3181 ccgtcgtcaa ccgcgagcag gccgcgctcg gcgacgtcgt cggcctgatc gacaccggcc
     3241 cgcactgcgt gctgcgcgtc cagccggccg acccggcggc cgacgagcgc ctgatcccct
     3301 tcgtcgaagc ctacgtcgac tccgtcgaca tgcccggccg ccgcatcgtg gtggactggg
     3361 gcctcgactt ctgacgcccg gagcgcccgc atgcgcttcg acgtcgtcac gctgttcccc
     3421 gacctgttcg cgcccttcct gacgcagggc atcacgcgcc gcgcctacga atcgggccag
     3481 gtggacgtga agctgtggaa cctgcgcgac cacgccgagg gtacctaccg ccgcgtcgac
     3541 gaccgcccgt tcggcggcgg ccccggcatg gtgatgctgg ccgagccgct ggaacgcgcg
     3601 ctgtcggccg tcgcgagcgc gcgcgcgggc gcaggcgcgc ccgcggggcc ggtcgtgctg
     3661 ttcacgccca ccggccgccg catcgaccag gcgctggtgc gcgagtgggc cgacggcccc
     3721 ggcgccacgc tgatctgcgg ccgctacgag ggcatcgacc agcgcgtcgt cgaccgccgc
     3781 gtcgacatcg agctgagcct gggcgacttc gtgctgtccg gcggcgagat ccccgcgctg
     3841 gcgctgctgg acgcgattgc gcgcctgatg ccgggcgtgc tcggcgacga gcagtcgcac
     3901 ctgcaggaca gcttcgagca gggcctgctc gactgcccgc actacagccg gcccgagatc
     3961 ctggccaccc ccgagggcga actgccggtg cctccggtgc tgctgtcggg ccatcacaag
     4021 gacatcgcgc gctggcgccg tgagcagtcg ctcaccgtga cggcccgtcg ccgtcccgac
     4081 ttgatcgccg ccgcccgggc cgcggggcag ctgacgaagg ccgacgagaa gttcctggcc
     4141 aacctgccgc cgaacacgct ataatctcgg gctctgttcc tctgccgggc agaagcagct
     4201 gacggaaggc cgcaagccgc cgtcgcctgg aaggactcca gtgctgcact ccaagcaatc
     4261 gtgcgccggc aagaacggat gattctaagg tttcaccatg gatctgatcg ctaccctcga
     4321 gcaggaagag attgcccgcc tcggcaagaa cattccggct ttcgcccccg gtgacacggt
     4381 catcgtcaac gtcaacgtcg tcgaaggcac gcgcaagcgc gtccaggcgt acgaaggcgt
     4441 cgtgatcgcc cgtcgcaacc gcggcctgaa ctcggctttc atcgttcgca agatctcc
//
LOCUS       DATCRO010000598         6402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_400540, whole genome shotgun sequence.
ACCESSION   DATCRO010000598 DATCRO010000000
VERSION     DATCRO010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400540"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..841)
                     /locus_tag="VIN75_16320"
     CDS             complement(74..841)
                     /locus_tag="VIN75_16320"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY8975787.1"
                     /translation="MSTLTTVLVVRPRAQAAAWVADLAALGVAARALPLIDILPSPEP
                     ARVAEAFAAVAGSKAEPVLVFVSPNAVHGFFDAAAATAWPVQARAACTGPGTVAALRE
                     RGVPAGHIVAPDADAPRFDSEALWSLVAAWPWAARPVWIVRGNGGRDWLGDRLRAAGA
                     DVRVVQSYGRAAPSLAPAERALLDAALAEPARWTWMFSSSEAIDNLQGLAPSAGWSAS
                     TALASHPRIAERARRLGFGTVTIVPPSPAAVAAVLAA"
     gene            complement(908..1885)
                     /gene="hemC"
                     /locus_tag="VIN75_16325"
     CDS             complement(908..1885)
                     /gene="hemC"
                     /locus_tag="VIN75_16325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY8975788.1"
                     /translation="MNNIVIATRESRLALWQARHVQALLAGMGVTASLLGMTTRGDQI
                     VDRTLSKVGGKGLFVKELETALESGDAHLAVHSLKDVPMELPPGFELVAVLDREDPRD
                     AFVSNGYASLADLPPGALVGTSSLRRVAQLLALRPDLRVAPLRGNLDTRLRKLDEGGY
                     DAIVLAAAGLVRLGLGDRIRARFDVGQMIPAAGQGALGLEIRADNASLHDRLVPMIAA
                     DTWLAVHAERAVARTLGGSCSMPLAAFATREADGRFTLRAALGDAAVGAALETGIGQE
                     GPRVDARPLLKASETAALADAAAAEAMGRRVAAALLAAGGDAYLPPAGA"
     gene            2004..4835
                     /gene="ppc"
                     /locus_tag="VIN75_16330"
     CDS             2004..4835
                     /gene="ppc"
                     /locus_tag="VIN75_16330"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY8975789.1"
                     /translation="MPRTAAARTPAKAPRKTAAAKPAPSTVDKDRPLVEDIRLLGRLL
                     GEVIREQEGLAAFELIERVRQLSVAYRIHKDASAGRVLDRLLKNLSADQTVSVIRAFS
                     YFSHLANIAEDRQHVRRRAHHEREGHLQDGSLAMAFERLHEADHRATEIAAALNKAFI
                     SPVLTAHPTEVQRKSILDAERAIAELLAERADLYTERALNENTALMRARITQLWQTRM
                     LRYTKLTVADEIENALSYYHATFLRQIPRMYKAIEDALPGQDIAPFFRMGNWIGGDRD
                     GNPNVTAETLQAALSRQSETALRHYLYEVHALGAELSISQMLAPVTPEMVALAASSPD
                     HNVHREDEPYRRALVGIYARLAATLHHLTGTEALRHAVVPQDPYGSAAEFSADLAVIE
                     RSLRSHHADALIAPRLAPLQRAVQVFGFHLATVDLRQSSDQHEAVIVELLRTARVEQD
                     YGALDEGARRELLLKLLNDARPLRVHGAQYSPLALGELAIFEAAGRMRARFGVEAIRH
                     YIISHTEDVSDLLEVLLLQKECGLMRGTLDGEAVADLIVSPLFETIADLRNGAPIMQQ
                     FYALPGIAALVKRSGAEQDIMLGYSDSNKDGGFFTSNWELYRTEVALVQLFAQLEEAH
                     GIRLRLFHGRGGTVGRGGGPSYQAIVAQPPGTVNGQIRLTEQGEVIASKYANPEIGLR
                     NLETLVAATLEATLLHTTKTPPKAFLDAADELSQASFDAFRALVYETPGFTDYFFSAT
                     PIREIAELNIGSRPASRKSTRRIEDLRAIPWGFSWGQCRVALPGWCGFGSAVQAFLGA
                     PGDEHERKLELLRKMYRQWPFFRTLLSNLDMVLAKSDLRIAARYVDLVDDRKLGKRIF
                     GAIKAEWERTQAMLGEITGEAHRLQSNPSLARSIEHRFPYLDPLNHLQVELMRRYRMR
                     KADDPSTERVKRGIHLSINGIAAGLRNTG"
     gene            5077..>6402
                     /locus_tag="VIN75_16335"
     CDS             5077..>6402
                     /locus_tag="VIN75_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975790.1"
                     /translation="MRQVLDVESRYGADPVETIEHLRPLEAAARASGGDDLRTFLAAW
                     GYAHAATDKPAVADAAVEELTDLGERTRNPGALASAYTLKASQLEFAGQVRAAFGWIE
                     EALPYARQDPSPDLHYWVEMTAGDLAMQNGQLEEGMRLFEAAAKSGRDMDNPRREAQA
                     WVSLVPMRIVKGDIGAALKEAVLVRELGGRSTDRGLVVAGWVMESLAAAAAGQPERAA
                     NARAQAVRTQKEIGQALGIAASSASAVQSINSLAGQPEWLSSEQDALMSLAGMYLDVH
                     DWARAAEAATRARQQSESRHDGDTAARALINLGMADIGLGHVQLGKTEVDAGLRAMEA
                     VKRDPELLGQINRYVALLERSGETREALVRLRQSLLLENELARRDRTSTVVALQRQSS
                     FEQHQRHMEQLEHENALQTVELQRRSTERTLMIALAAVLAIGVAVAASLY"
BASE COUNT          963 a         2385 c         2254 g          800 t
ORIGIN      
        1 ccacatggcc gcgggccgcc gccgccgggc aacgggggca gccgatgcgc gcccgccaca
       61 cgcggcgcga ggctcaggcg gcgagcacag cggccacggc cgcgggggac ggtgggacga
      121 tcgtcaccgt gccgaagccc agccggcgcg cacgctcggc gatgcgcgga tgcgaggcca
      181 gcgcggtgct ggcgctccag ccggccgatg gcgccagccc ctgcaggttg tcgatggcct
      241 ccgagctgct gaacatccac gtccagcgcg cgggctcggc gagcgccgcg tccagcaacg
      301 cgcgctccgc gggcgccagc gagggcgcgg cgcggccgta ggactgcacg acgcgcacgt
      361 ccgcgccggc ggcgcgcaat cggtcgccca gccagtcgcg gccgccgttg ccgcgcacga
      421 tccagacggg ccgcgcggcc cagggccagg cggcgacgag cgaccacagc gcctcggagt
      481 cgaaccgggg cgcgtccgca tcgggcgcca cgatgtgccc ggccggcacg ccgcgttcgc
      541 gcaaggcggc gacggtgccg gggcccgtgc aggcggcccg ggcctggacg ggccaggcgg
      601 tcgccgcagc ggcgtcgaag aagccgtgca ccgcgttcgg gctgacgaag acgagcaccg
      661 gctcggcctt cgagccggcc acggccgcga acgcctcggc cacgcgcgcc ggctcgggcg
      721 aggggaggat gtcgatcagc ggcagcgcgc gcgccgcgac gccgagcgcc gcgaggtcgg
      781 ccacccatgc ggccgcctgc gcccgcggac gcacgaccag caccgtcgtc agcgtgctca
      841 tgggccagcc gcgtgctgcg cacgtcggaa ttcgcccttc gggcccccgg gcgagctcat
      901 ggccggctca cgcgccggcg ggcggcaggt aggcgtcgcc gccggcggcc agcagcgcgg
      961 cggccacgcg cctgcccatc gcctcggcgg ccgcggcgtc ggccagcgcc gccgtctcgc
     1021 tcgccttcag cagcgggcgg gcgtccacgc gcgggccttc ctggccgatc ccggtttcca
     1081 gcgcggcgcc gaccgcggcg tcgccgagcg cggcgcgcag cgtgaagcgg ccgtcggcct
     1141 cgcgcgtggc gaacgccgcc agcggcatgc tgcagctgcc gcccagcgtg cgcgcgacgg
     1201 cgcgctccgc gtgcacggcg agccaggtgt cggcggcgat catgggcacg aggcgatcgt
     1261 gcagcgacgc gttgtcggcg cggatctcca ggccgagcgc gccctggcct gccgccggga
     1321 tcatctgccc gacgtcgaag cgcgcgcgga tgcggtcgcc cagacccagc cggacgagcc
     1381 cggcggcggc cagcacgatg gcgtcgtagc cgccttcgtc gagcttgcgc aggcgggtgt
     1441 cgaggttgcc gcgcagcggg gccacgcgca ggtcgggccg cagcgccagg agctgcgcca
     1501 cgcgacgcag gctggaggtg cccaccagcg cgccgggcgg caggtcggcg agcgaggcgt
     1561 atccgttgga gacgaacgcg tcgcgcggat cctcgcgatc gagcaccgcg acgagctcga
     1621 agccgggcgg cagctccatc ggcacgtcct tgagcgagtg cacggccagg tgcgcgtcac
     1681 ccgattccag cgcggtttcg agttccttga cgaacaggcc cttgccgccc accttggaca
     1741 gcgtgcggtc gacgatctgg tcgccacggg tggtcatgcc caggagcgac gcggtgacgc
     1801 ccatgccggc cagcagcgcc tgcacgtgcc gggcctgcca cagcgcgagc cggctttcgc
     1861 gggtggcgat cacgatgttg ttcattcgcg gattatcgcc atcgacttcg ttgcctgtca
     1921 gcgaaagaag ctcttcccgc cgcgaaggct gcctgctaca tttggcgatt ggtaattcgg
     1981 ttacatcaaa cgaaagcctt tcgatgcctc gcaccgccgc cgcccgcacg cccgccaaag
     2041 cgccccgcaa gaccgcggcc gccaagcccg ctccctcgac ggtcgacaag gatcgcccgc
     2101 tggtcgagga catccgcctg ctcggacgcc tgctcggaga ggtcatccgg gaacaggaag
     2161 gactggcagc cttcgagctg atcgagcgcg tgcgccagct gtccgtggcc taccgcatcc
     2221 acaaggacgc gagcgccggg cgcgtgctcg atcgcctgct caagaacctg tcggccgacc
     2281 agacggtcag cgtgatccgc gcgttcagct acttctcgca cctcgccaac atcgccgagg
     2341 atcgccagca cgtgcgccgc cgcgcgcacc acgagcgcga aggccacctg caggacggct
     2401 cgctcgcgat ggcgttcgag cgcctgcacg aggccgacca ccgcgccacc gagatcgccg
     2461 cggcgctgaa caaggccttc atctcgccgg tgctcacggc gcacccgacc gaggtgcagc
     2521 gcaagagcat cctggacgcc gagcgcgcga tcgccgagct gctggccgag cgcgccgacc
     2581 tctacaccga gcgcgccctc aacgagaaca ccgcgctgat gcgggcccgc atcacgcagc
     2641 tgtggcagac gcgcatgctg cgctacacca agctgacggt ggccgacgag atcgagaacg
     2701 cgctgtcgta ctaccacgcc accttcctgc gccagatccc gcgcatgtac aaggcgatcg
     2761 aggacgccct gcccggccag gacatcgcac ccttcttccg catgggcaac tggatcggcg
     2821 gcgaccgcga cggcaacccc aacgtcacgg ccgagacgct gcaggccgcg ctgtcgcgcc
     2881 agagcgagac cgcgctgcgc cactacctgt acgaggtgca cgccctgggc gcggagctgt
     2941 cgatctcgca gatgctcgcg cccgtcacgc ccgagatggt ggcgctggcc gcgagctcgc
     3001 ccgaccacaa cgtgcaccgc gaggacgagc cctatcgccg cgcgctggtg ggcatctacg
     3061 cgcgcctggc cgccacgctg caccatctca ccggcaccga ggcgctgcgc cacgcggtgg
     3121 tgccgcagga tccgtacggc tcggccgcag agttcagcgc cgacctggcg gtcatcgagc
     3181 gttcgctgcg ctcgcaccac gccgacgcgc tgatcgcccc gcgcctggcg ccgctgcaac
     3241 gcgcggtgca ggtgttcggc ttccacctgg ccaccgtgga cctgcgccag agctccgacc
     3301 agcacgaggc cgtgatcgtc gagctgctgc gcaccgcgcg cgtcgagcag gactacggcg
     3361 cgctcgacga aggcgcgcgc cgcgagctgc tgctgaagct gctgaacgac gcccgcccgc
     3421 tgcgcgtgca cggcgcgcag tactcgccgc tggcgctcgg cgaactggcc atcttcgagg
     3481 cggcgggcag gatgcgcgcg cgcttcggcg tcgaggcgat ccgccactac atcatctcgc
     3541 acaccgagga cgtgagcgac ctgctggagg tgctgctgct gcagaaggaa tgcggcctga
     3601 tgcgcggcac gctcgacggc gaggcggtgg ccgacctgat cgtctcgccg ctgttcgaga
     3661 ccatcgccga cctgcgcaac ggcgcgccca tcatgcagca gttctacgcc ctgccgggta
     3721 tcgcggcact ggtcaagcgc agcggcgccg agcaggacat catgctgggc tacagcgaca
     3781 gcaacaagga cggcggcttc ttcaccagca actgggagct gtaccgcacc gaggtcgcgc
     3841 tggtgcagct gttcgcgcag ctggaagagg cgcacggcat ccgcctgcgc ctgttccacg
     3901 gccgcggcgg caccgtcggc cgcggcggcg gcccgagcta ccaggccatc gtcgcccaac
     3961 ccccgggcac cgtgaacggc cagatccgcc tgaccgagca gggcgaggtg atcgcgtcca
     4021 agtacgcgaa ccccgagatc ggcctgcgca acctcgagac gctggtggcc gccacgctcg
     4081 aagccaccct gctgcacacc accaagacgc cgcccaaggc gttcctggac gcggccgacg
     4141 agctgagcca ggccagcttc gacgcgttcc gcgcgctggt gtacgagacc ccgggcttca
     4201 cggactactt cttctccgcg acgcccatcc gcgagatcgc cgagctcaac atcggctcgc
     4261 gcccagcctc gcgcaagtcc acgcgccgca tcgaggacct gcgcgccatc ccgtggggct
     4321 tctcgtgggg ccagtgccgc gtcgccctgc ccggctggtg cggcttcggc tccgccgtcc
     4381 aggccttcct gggcgcgccg ggcgacgagc acgagcgcaa gctggaactg ctgcgcaaga
     4441 tgtacaggca gtggccgttc ttccgcacgc tgctgtccaa cctggacatg gtgctggcca
     4501 agagcgacct gcgcatcgcg gcgcgctacg tcgatctggt ggacgacagg aagctgggca
     4561 agcgcatctt cggcgcgatc aaggcggagt gggaacgcac gcaggccatg ctgggcgaga
     4621 tcaccggcga ggcgcatcgc ctgcagtcca acccgtcgct ggcgcgctcc atcgagcacc
     4681 gcttccccta cctcgacccg ctgaaccacc tgcaggtcga gctcatgcgg cgctaccgca
     4741 tgcgcaaggc ggacgatccg tccaccgagc gcgtcaagcg cggcatccac ctgtcgatca
     4801 acggcatcgc cgcgggcctg cgcaacacgg gctgaacggg cgtgaaaaag tcacgcccgg
     4861 cgggccgggt gttgggaact ccccgcttca attagcgcgg cttcgggtcg ataaggcgat
     4921 gtggcctcct cgcatcgccc cctgacagtc tcgtcgccgc cccgcgcccg cgcgggccgg
     4981 cgcccggccg atcgtggccg cctgctggcg atcgcggcgt cgctggtgtt gctgggcgcc
     5041 ttcgcggccc gcccggcaca cgccgccgaa tcgatcatgc ggcaggtgct ggacgtcgag
     5101 tcccgctacg gcgccgaccc ggtcgagacc atcgaacacc tgcgcccgct cgaggccgcc
     5161 gcgcgcgcca gcggcggcga cgacctgcgc accttcctgg ccgcctgggg ctacgcccac
     5221 gccgccaccg acaagcccgc ggtcgcggac gccgccgtcg aggaactgac ggatctgggc
     5281 gagcgcacgc gcaatcccgg cgcgctggct tcggcctaca cgctcaaggc ctcgcagctg
     5341 gagttcgcgg gccaggtccg cgccgcattc gggtggatcg aggaagcctt gccctacgcg
     5401 cgccaggatc ccagcccgga cctgcactac tgggtcgaga tgaccgccgg cgacctcgcg
     5461 atgcagaacg gccagctcga ggaaggcatg cgcctgttcg aggcggccgc gaagtcgggc
     5521 cgcgacatgg acaacccgcg ccgcgaggcc caggcctggg tctcgctggt gccgatgcgc
     5581 atcgtcaagg gcgacatcgg cgccgcgctg aaggaagccg tgctcgtgcg cgagctcggc
     5641 ggccgctcga ccgaccgcgg cctggtcgtg gccggctggg tgatggagtc gctggccgcc
     5701 gccgccgcgg gacagcccga gcgcgcggcg aacgcccgtg cgcaagccgt gcgaacccag
     5761 aaggagatcg gccaggcatt gggcatcgcc gcgtcctccg cgtcggccgt gcagtcgatc
     5821 aattcgctgg cgggccagcc cgaatggctc agcagcgaac aggacgcgct gatgagcctt
     5881 gccggcatgt acctggacgt gcacgactgg gcccgggccg ccgaggcggc cacgcgcgcc
     5941 aggcagcaat ccgaatcccg gcatgacggc gacaccgccg cacgcgcgct gatcaacctg
     6001 ggcatggccg acatcggcct ggggcacgtc cagctcggaa agaccgaagt ggacgccggc
     6061 ctgcgggcga tggaggccgt caagcgcgac cccgagctgc tgggccagat caaccgctac
     6121 gtcgcgctgc tcgagcgcag cggcgagaca cgcgaggcgc tcgtgcgcct gcgccagtcg
     6181 ctgctgctgg agaacgagct tgcgcggcgc gaccgcacga gcaccgtcgt cgcgctgcag
     6241 cgccagtcca gcttcgagca gcaccagcgc cacatggagc agctggagca cgagaacgcg
     6301 ctgcagacgg tggaactgca gcgccgctcc accgagcgca cgctgatgat cgcgctggcc
     6361 gccgtgctgg cgatcggcgt cgcggtcgcc gcgagcttgt ac
//
LOCUS       DATCRO010000599         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_715501, whole genome shotgun sequence.
ACCESSION   DATCRO010000599 DATCRO010000000
VERSION     DATCRO010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715501"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VIN75_16340"
     CDS             <1..723
                     /locus_tag="VIN75_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8975791.1"
                     /translation="GHRCDIVTMFDRGLLALQGPMAVHAIARLSPGIEKLTFMTGGFF
                     KVGGIPCYVTRSGYTGEDGFEISVAAADTDALARKLLEQPEVKPVGLGARDSLRLEAG
                     LCLYGNDIDATTTPVEAGLTWAIQKVRRPGGERENHYPGADIIGKQLAVGARTKRVGL
                     VGLERAPVREGTIIVDHHGHKLGVVTSGTLGPTVNQPVAMAYLPVDHAIVAHEVYAEV
                     RGKRLPMRVTPMPFAPHRYFRG"
     gene            785..1159
                     /gene="gcvH"
                     /locus_tag="VIN75_16345"
     CDS             785..1159
                     /gene="gcvH"
                     /locus_tag="VIN75_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8975792.1"
                     /translation="MTTKFTTDHEWINIEDHDAAVVGITLHAQDALGDVVFVDLPAVG
                     RSFAKGEIAGVVESVKAAADIYMPVSGEVVEVNEKLRDEPALANTDPLGEGWFFKVKI
                     ANMAEFDELMDAPAYDALLKTL"
     gene            1286..>3348
                     /gene="gcvP"
                     /locus_tag="VIN75_16350"
     CDS             1286..>3348
                     /gene="gcvP"
                     /locus_tag="VIN75_16350"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY8975793.1"
                     /translation="MSLTATTPLAELENATEFVARHIGPEAADEARMLSAIGAASREA
                     LISALVPGNILRGQSMAIPDAVTEANALAELKAVASKNKVLKSFIGQGYHGTITPGVI
                     LRNILENPAWYTAYTPYQAEISQGRMEALLNFQTMVTDLTGMAIANASMLDEATAAAE
                     AMTLAQRMGKSKSKVFFVADDVLPQTLEVVRTRARPLGIEVVVGGAAQAGEKDCFAAL
                     FQYPGVTGQVHVLKPVVDAVHARGGLAIVAADLLALTLLQAPAEFGADIACGNTQRFG
                     MPMGCGGPHAAYLATQDALKRSLPGRLVGVSVDAHGAPAYRLALQTREQHIRREKATS
                     NICTAQVLPAVIASMYAVYHGPAGLRRIARRVASFAAIFAAGLAQRGLSVVNAHAFDT
                     IEIATGPQTDAVLARAVAAGMNLRRASADSVSVTFDECTTRACLTALWGVFAPGQALP
                     SFDDFEQGVALRLPVELLRTSDFLTHPVFNTHHSETEMLRYIRRLSDKDLALDRSMIP
                     LGSCTMKLNATSEMIPITWPEFAHVHPFAPADQLEGYRLLNDQLCKWLEQATGYAGIS
                     LQPNAGSQGEYAGLLVIKAWHESRGQGARDVCLIPESAHGTNPASAQMVGMKVVVVKC
                     TAEGEVDMADLEAKCVAHKDDLAAMMITYPSTYGVFEPGVKALCALVHRYGGRVYVDG
                     ANMNA"
BASE COUNT          518 a         1265 c         1104 g          461 t
ORIGIN      
        1 ggccaccgct gcgacatcgt cacgatgttc gaccgcggcc tgctcgcgct gcagggcccg
       61 atggccgtcc atgcgatcgc gcgcctgtcg ccgggcatcg agaagctgac gttcatgacc
      121 ggcggcttct tcaaggtggg cggcatcccc tgctacgtca cgcgctcggg ctacaccggc
      181 gaggacggct tcgagatctc ggtcgccgcg gccgacaccg acgccctcgc ccgcaagctg
      241 ctcgagcagc ccgaggtcaa gcccgtcggc ctgggcgcgc gcgactcgct gcgcctggaa
      301 gccggcctgt gcctgtacgg caacgacatc gacgccacca ccacgccggt cgaggccggg
      361 ctcacctggg ccatccagaa ggtgcgccgc ccgggcggcg agcgcgagaa ccactacccc
      421 ggcgccgaca tcatcggcaa gcagctggcc gtgggcgcgc gcaccaagcg cgtcggcctg
      481 gtcggcctgg agcgcgcgcc ggtgcgcgaa ggcacgatca tcgtcgacca ccacgggcac
      541 aagctgggcg tcgtcaccag cggcacgctg ggcccgaccg tgaaccagcc ggtggccatg
      601 gcctacctgc cggtcgacca tgccatcgtc gcgcacgaag tctacgccga ggtgcgcggc
      661 aagcgcctgc cgatgcgcgt gacgccgatg ccgttcgcgc cgcaccgcta cttccgcggc
      721 tgagcccacc gctcgtccgc cttccgattc cttcccgcct ttggcctttt tccaggagcc
      781 ctgcatgacg accaagttca cgaccgacca cgaatggatc aacatcgaag atcatgacgc
      841 cgccgtcgtg ggcatcacgc tgcatgccca ggacgcgctc ggcgacgtcg tgttcgtcga
      901 cctgcccgcc gtcggccgct cgttcgccaa gggcgagatc gccggtgtcg tcgagtcggt
      961 caaggccgcc gccgacatct acatgccggt ctccggcgag gtcgtcgagg tcaacgagaa
     1021 gctgcgcgac gagccggcgc tggccaacac cgacccgctg ggcgaaggct ggttcttcaa
     1081 ggtgaagatc gccaacatgg ccgagttcga cgagctgatg gacgcgccgg cctacgacgc
     1141 gctgctcaag acgctctgac ccctgccccg ctgccgaccg cgcgcggcgt gaccgcgtcg
     1201 cgccgtccga gcgcctgacc ggccgcgtcc atccgacgcg cgccgggcgc ttcctgccct
     1261 atcccctgca cgcctggaat tgcccatgtc cctgaccgcc accaccccgc tggccgagct
     1321 cgagaacgcc accgaattcg tcgcccgcca catcggcccc gaggccgccg acgaggcgcg
     1381 catgctctcg gccatcggcg ccgcctcgcg cgaggcgctg atctcggccc tggtgcccgg
     1441 gaacatcctg cgcggccagt cgatggcgat cccggacgcg gtcaccgagg ccaacgcgct
     1501 ggccgagctg aaggccgtcg cctcgaagaa caaggtgctc aagagcttca tcggccaggg
     1561 ctaccacggc acgatcacgc cgggcgtcat cctgcgcaac atcctcgaga acccggcctg
     1621 gtacaccgcc tacacgccct accaggcgga gatctcgcaa ggccgcatgg aagcgctgct
     1681 gaacttccag accatggtca cggacctgac gggcatggcc atcgccaacg cctcgatgct
     1741 ggacgaagcc accgccgccg ccgaggcgat gacgctggcc cagcgcatgg gcaagagcaa
     1801 aagcaaggtg ttcttcgtcg ccgacgacgt gctgccgcag acgctggagg tcgtgcggac
     1861 ccgcgcccgg ccgctgggca tcgaggtggt ggtcggcggc gccgcgcagg ccggcgagaa
     1921 ggactgcttc gcggcgctgt tccagtaccc gggcgtcacc ggccaggtgc acgtgctcaa
     1981 gcccgtcgtc gacgcggtgc acgcgcgcgg cggcctggcc atcgtcgccg ccgacctgct
     2041 ggccctgacg ctgctgcagg ccccggccga attcggcgcc gacatcgcct gcggcaacac
     2101 gcagcgcttc ggcatgccca tgggctgcgg cggcccgcac gccgcctacc tcgccacgca
     2161 ggacgcgctc aagcgctcgc tgccgggccg cctggtgggc gtgtccgtcg acgcgcacgg
     2221 cgcgccggcc taccgcctcg cgcttcaaac gcgcgagcag cacatccgcc gcgaaaaggc
     2281 cacgtccaac atctgcacgg cgcaggtgct gccggcggtc atcgccagca tgtacgccgt
     2341 ctaccacggc cccgcgggcc tgcgccgcat cgcgcgccgc gtcgcgtcgt tcgcagccat
     2401 cttcgccgcc ggcctcgccc agcgcggcct ctcggtggtc aacgcgcacg cgttcgacac
     2461 gatcgagatc gccaccggcc cgcagaccga cgccgtgctc gcgcgcgccg tcgccgccgg
     2521 catgaacctg cgccgcgcca gcgcggacag cgtgtcggtc accttcgacg aatgcaccac
     2581 gcgcgcctgc ctgaccgcgc tgtggggcgt gttcgcgccg ggccaggcgc tgccgtcgtt
     2641 cgacgacttc gagcagggcg tggcgctgcg cctgcccgtc gagctgctgc gcaccagcga
     2701 cttcctcacg cacccggtgt tcaacacgca ccacagcgag accgagatgc tgcgctacat
     2761 ccgccgcctg tccgacaagg acctggcgct ggaccgcagc atgatcccgc tgggctcgtg
     2821 cacgatgaag ctcaacgcca ccagcgagat gatcccgatc acgtggcccg agttcgcgca
     2881 cgtgcacccg ttcgcgccgg ccgaccagct cgagggctac cggctgttga acgaccagct
     2941 ttgcaagtgg ctcgaacagg ccacgggcta cgccggcatc agcctgcagc ccaacgccgg
     3001 ctcgcagggc gagtacgccg gcctgctggt gatcaaggcc tggcacgagt cgcgcggcca
     3061 gggcgcgcgc gacgtctgcc tgatccccga gtcggcgcac ggcacgaacc cggcgtcggc
     3121 gcagatggtg ggcatgaagg tcgtcgtcgt gaagtgcacg gccgagggcg aggtcgacat
     3181 ggccgacctc gaggccaagt gcgtcgcgca caaggacgac ctggccgcga tgatgatcac
     3241 ctacccgtcc acgtacggcg tgttcgagcc gggcgtcaag gcactgtgcg cgctggtgca
     3301 ccggtacggc ggccgcgtct acgtcgacgg cgccaacatg aacgcgat
//
LOCUS       DATCRO010000600         9614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_153131, whole genome shotgun sequence.
ACCESSION   DATCRO010000600 DATCRO010000000
VERSION     DATCRO010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153131"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2462)
                     /locus_tag="VIN75_16355"
     CDS             complement(<1..2462)
                     /locus_tag="VIN75_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEY8975794.1"
                     /translation="MNKALTAALLSAGVLSLASLAAQVTAADTPAAPALPAAHPTLPA
                     NAGAILAAASPASAAIARAWAYTPVQKPAVPAVKQAAWVRTPLDAFVLAKLEAKGLTP
                     SPDADRATFIRRATLDAWGLIPTPEDVKAFVNDKSPKAYEKLVDRLLASPHYGERQGR
                     RWLDLTRYADSDGYNADGTRPNIWRYRDYVIASFNQDKPFNRFVQEQIAGDELWPDRN
                     EALIATGFLRNFPDEINARDLNLKKQEVANDLTDTVSSVFLGTTLGCAQCHDHKFDKI
                     SQKEYYQFQAFFVNASWNDDVKALEGPAKAEWLARQARWEAATKDIRDQQDALLRPVI
                     EKLESDRLSGFVPDTRASITKPEAERDAYDRWIYHRNLWTLSGRTRNALADLKKKDKD
                     GYAKYQALDEQLKAFDAIKPKDPGNISTMSELGHSDAPRTYVLFKGIYDRRLDEVQPG
                     FPSLLTDAKPQIVPTATSSGRRSAVANWLVSPDNPLTARVFVNRQWAQVFGNGIVESV
                     SDFGKMGTKPTNPELLDYLSAEFVQDGWSVKKLQREILLSHTYRQSSSPRSDAEKVDP
                     TNKLLWAFPRQRLEAEEIRDSLLEASGLLEDKLGGPAVFPPLPAGTQAPNRNFWITSD
                     NPHDANRRSVYVFIRRNLPYPLLDTFDWANPQIVHNKRDVTTTAPQALALINSDLVYG
                     WSKALAGRVIKEQPKASDSARIDRVYEILYSRKPDAFEKTRLLAFLKTQQELEARQLA
                     GGKKVNLPEGYGVAPSLESQVDRLYKVAYGREPDRFEQAALVEFIEKQKQKETRWATA
                     PNPNYDPAALAAAAAAANAAANT"
     gene            complement(2706..5318)
                     /locus_tag="VIN75_16360"
     CDS             complement(2706..5318)
                     /locus_tag="VIN75_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8975795.1"
                     /translation="MSRSTSRQQQQQHARSPFHLLPVAAAVLAMTMSGNAQAQSATAA
                     APAASAADATNEAPALDAVTVHSRNRIERLQDVPVSVSVVTGAELERLDAYGVGAIAK
                     RAANVSWNVGNQRTSSLSIRGIGKIGQTEAQDPSVGVIFDGVSYGYNALTSSFDFVDI
                     DTIEVARGPQGTLQGKNASVGSITINTKRPSFTPTADYTLAFNKNNGVLGVAAIGGPV
                     IDDLLAWRGTFSVDRQQGARANAYNRDQTYTNTDRVSGRVQLLLKPDDSFSARLEGDV
                     QPRAGETTNGNTKFSPTPTQYADGVTNTLSTDVSTRLARSWFTNNPGYDINDYYAGID
                     NDAQHALVTGSNGLTTEVNWRIPSGQTLTSITAYKSYHFNAVNDDGTPFDIYRNAGGF
                     WNDYHQFSQELRISSPVGGFLDYQAGLYFIKVNNSSTYQRAWGTDAGAWFASTAQYNT
                     LDSTAAGQLLMRDSLANVSMAYNSPAGQQTIENKSGAVFAQANWHFTDRLTLTTGARL
                     THENRTNVSSSYVINPGDGVDLANFNDPATRDAAAAKYFGTGRTYASLTAAELAQVKA
                     AQAIRTSNLGVLFNSTGAKPFRANQPALVLSPSYKISPDVTSYASYQHGEKAGIAQLV
                     NGVSTPAAAEKTNSFELGLKSALLDKTLILNADIFDTQIHNYQQAVQVVDQYTTTSKN
                     DGNTYYTSATGNAPRVGVTGLEIDGVYGGIARTTLRFSAAYNRAVYKDFPNSPQPVEN
                     GDLTTAVPGVHPAQPYRSVDGKTLAGAPRFTFNVGADYNQPIASDKVGHVSANLAYST
                     GYNTDVALSKYATVGAQALVDLAVGAGKRDKSFDVSVIVKNLFNNETPQSRTWNSITP
                     ATPRTYGIQFTGKL"
     gene            5665..6195
                     /locus_tag="VIN75_16365"
     CDS             5665..6195
                     /locus_tag="VIN75_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8975796.1"
                     /translation="MTTPRFLVAALLASAFASASAQEAAPQQPWRVAGGASDPAAVKR
                     VQESLAAPWKYKARRLTRAEVDALLAKPESVVFIDLRRPDEFITYGSFPVFISVQNQD
                     LEKQLAWLPHDKTIVTVSNHAQRAGAAADLLAAKGFNVAGATGAEEYEVQGGTNVAHV
                     APPVRRAAAAASTPRS"
     gene            6208..7005
                     /locus_tag="VIN75_16370"
     CDS             6208..7005
                     /locus_tag="VIN75_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8975797.1"
                     /translation="MSSPELMSDFGVAEPAGAACDEVHRAVLWDLPLRVFHWSLVAAV
                     TVAIVTGELGGAWMPWHGRAGLVIVGLLVFRLLWGVLGSATSRFTQFAPTPARLRAYL
                     RGRWRGVGHNPLGALSVFALLGLLSLQAATGLFGNDDIAFAGPLNHLVDDAAGARATG
                     WHRWLANGLIALIALHLLAIAFHVLVKRHRLVRPMITGRHALEPGGDWPRPRSYGGFA
                     LLSAVAVAATSVLALAGAGETPVASPAAPARAALVAAPAHAASTPAW"
     gene            complement(7007..7591)
                     /locus_tag="VIN75_16375"
     CDS             complement(7007..7591)
                     /locus_tag="VIN75_16375"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8975798.1"
                     /translation="MKHTLASSLALAALALSMPAARAALSAGDAAPPFTAQASLAGRT
                     FTFSLADAVKRGPVVVYFYPSAYTGGCNLEAHTFATRQAEFAAAGASIIGVSLDDIGR
                     LNAFSADPDYCAGKIPVASDTGGRVAHAYALAVREAPGRKDTRGQQIEHGLAERSTFV
                     VTPDGRIAAAFDGLSPVENVTQALAAVQRLAPGH"
     gene            7816..9003
                     /locus_tag="VIN75_16380"
     CDS             7816..9003
                     /locus_tag="VIN75_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8975799.1"
                     /translation="MAKILTLPDSHTAPLTIRAKALVFHDPQSRLLLDHVERLARSDA
                     TVLVTGETGTGKELIARHIHATSGRTGPFVAVNCGAFSETLIDAELFGHETGAFTGAT
                     TARAGWFEAANGGTLFLDEIGDLPMALQVKLLRVLQERQVVRLGSRRPIALDVRLVAA
                     TNIDLARAVTAQHFRADLYYRLSVAGVRLPALHERQADILPLAEHFVGVYCARLGIRA
                     MPFSEDAVAALRSYSWPGNIRELENVIHYALIVCAGDAIQAQDLKLVPLQPPSGRRAG
                     DASPRDGHPGFGGYRPSEERERAAPPAPVPASARERLPAFLGELLREGGPELFDSVER
                     ALVHAAFEHAHGNQVRAARELGVTRNMVRTLLKRHGLLGCEPPIDASLSAAEGGPVAL
                     SAV"
     gene            9121..>9614
                     /locus_tag="VIN75_16385"
     CDS             9121..>9614
                     /locus_tag="VIN75_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8975800.1"
                     /translation="MTTPRLSRTLVALALAATAAVSFAKEITLLNVSYDPTRELYQDI
                     DASFPKYWKSKTGDDLKINASHGGSGAQSRAVQDGLDADVVTLALASDIDAIANKGLL
                     AKDWQKKFKDNSTPYTSTIVFLVRKGNPKGIHDWSDIAKPGVGVVVANPKTSGGARWA
                     YLAA"
BASE COUNT         1290 a         3136 c         3556 g         1632 t
ORIGIN      
        1 gtgttggccg cggcgttggc ggccgccgcc gccgcggcga gcgccgccgg gtcgtagttc
       61 gggttgggcg cggtggccca gcgcgtctcc ttctgcttct gcttctcgat gaactcgacg
      121 agcgcggcct gctcgaagcg atccggctcg cggccgtacg ccaccttgta gagcctgtcg
      181 acctggctct cgaggctggg cgccacgccg tagccttccg gcaggttcac cttcttgccg
      241 ccggcgagct gcctggcctc gagttcctgc tgggtcttca ggaacgcgag cagccgcgtc
      301 ttctcgaacg cgtcgggctt gcgcgagtac aggatctcgt agacgcggtc gatgcgcgcc
      361 gagtcgctcg ccttgggctg ctccttgatg acgcggcccg cgagcgcctt ggaccagccg
      421 tagaccaggt cgctgttgat cagcgccagc gcctgcggcg ccgtcgtggt cacgtcgcgc
      481 ttgttgtgca cgatctgcgg gttggcccag tcgaaggtgt ccagcagcgg atagggcagg
      541 ttgcgccgga tgaagacgta gacgctgcgg cgattggcgt cgtgcgggtt gtccgacgtg
      601 atccagaagt tgcggttcgg cgcctgcgtg ccggcgggca gcggcgggaa cacggcgggg
      661 ccgccgagct tgtcttccag cagccccgag gcctcgagca gcgagtcgcg gatctcctcg
      721 gcctccagcc gctggcgcgg gaaggcccac agcagcttgt tggtcggatc gaccttctcg
      781 gcgtcgctgc gcggcgagga ggactgccgg taggtgtgcg acagcaggat ctcgcgctgc
      841 agcttcttga cgctccagcc gtcctgcacg aactcggcgg agaggtagtc cagcagctcg
      901 ggattcgtcg gcttcgtgcc catcttgccg aagtcgctca ccgattcgac gatgccgttg
      961 ccgaacacct gcgcccactg gcggttgacg aacacgcgcg ccgtcagcgg gttgtccggc
     1021 gagacgagcc agttggccac cgcgctgcgg cggcccgacg acgtggcggt gggcacgatc
     1081 tgcggcttgg cgtccgtgag cagcgacggg aagcccggct gcacctcgtc gagccggcgg
     1141 tcgtagatgc ccttgaacag cacgtaggtc ctgggcgcat cgctgtggcc cagctcgctc
     1201 atcgtcgaga tgttgcccgg atccttgggc ttgatcgcgt cgaacgcctt gagctgctcg
     1261 tcgagcgcct ggtacttggc gtagccgtcc ttgtccttct tcttgaggtc ggccagcgcg
     1321 ttgcgcgtgc ggcccgacag cgtccacagg ttgcggtggt agatccagcg gtcgtaggcg
     1381 tcgcgctcgg cctcgggctt ggtgatggag gcgcgcgtgt cgggcacgaa tccggagagc
     1441 cggtcggact ccagcttctc gatgacgggc ctgagcagcg cgtcctgctg gtcgcggatg
     1501 tccttggtcg cggcctccca cctggcctgc ctggcgagcc attcggcctt ggcgggacct
     1561 tccagcgcct tgacgtcgtc gttccagctc gcgttcacga agaaggcctg gaactggtag
     1621 tactccttct gcgagatctt gtcgaacttg tggtcgtggc actgcgcgca gcccagcgtg
     1681 gtgccgagga acacggagct gacggtgtcg gtgaggtcgt tggcgacttc ctgcttcttc
     1741 aggttgaggt cgcgcgcatt gatctcgtcg gggaagttgc gcaggaagcc ggtggcgatc
     1801 agcgcctcgt tgcggtcggg ccagagctcg tcgccggcga tctgctcctg cacgaagcgg
     1861 ttgaacggct tgtcctggtt gaacgacgcg atgacgtagt cgcggtagcg ccaaatgttg
     1921 ggccgggtgc cgtcggcgtt gtagccgtcg gagtcggcgt agcgcgtcag gtcgagccag
     1981 cggcggccct ggcgctcgcc gtagtgcggc gacgcgagca ggcggtcgac gagcttctcg
     2041 taggccttgg gcgacttgtc gttgacgaag gccttgacgt cttccggcgt cgggatcagg
     2101 ccccacgcgt cgagcgtcgc gcggcggatg aaggtggcgc ggtcggcgtc gggcgagggc
     2161 gtgaggccct tggcttcgag cttggccagg acgaaggcgt ccagcggcgt cctgacccac
     2221 gccgcctgct tgacggcggg cacggcgggc ttctggacgg gcgtgtaggc ccaggcgcgc
     2281 gcgatcgcgg cgctcgcggg cgaagccgcg gcgaggatgg cgccggcgtt ggccggcagc
     2341 gtcggatggg cggcgggcag ggcaggtgcg gcgggcgtgt cggcggccgt cacctgagcg
     2401 gcgaggctgg ccagagacag gacgcctgcc gagagcaggg cggcagtgag ggctttgttc
     2461 attgggcgac tcggtgtcgg taagggaacg aacgtggcga ggaggcgggg gctgcagcgg
     2521 cgaggtggcc ctcgtggggc cgccgcgctg cgccatcacg gacggttacg caaatggcgc
     2581 gccagctttc gcacgggcat tgggcgtcgg atcgatggat tccatgcaga tccgctgaac
     2641 ggctgctgct ttcgccacac cgcgctcgca tggactggtg cgggggaagc agcgcgccgc
     2701 gggtgtcaga gctttccggt gaactggatg ccgtacgtgc gcggcgtcgc cggggtgatg
     2761 ctgttccacg tgcgcgactg gggggtctcg ttgttgaaca ggttcttgac gatcacgctg
     2821 acgtcgaagc tcttgtcgcg cttgcccgcg ccgaccgcca ggtcgaccag cgcctgcgcc
     2881 ccgacggtcg cgtacttcga cagcgcaacg tcggtgttgt agccggtgct gtacgcaagg
     2941 ttcgcgctga cgtgaccgac cttgtccgat gcgatcggct ggttgtagtc ggctccgacg
     3001 ttgaacgtga agcgcggtgc gccggccagc gtctttccgt cgacgctgcg gtagggctgg
     3061 gccggatgca cgcccggcac ggcggtggtg aggtcgccgt tctccaccgg ttgaggggag
     3121 ttcgggaagt ccttgtagac ggcgcggttg tacgccgccg agaaccgcag cgtggtgcgg
     3181 gcgatgccgc cgtaaacccc gtcgatctcc aggcccgtga cccccacgcg cggcgcattg
     3241 ccggtggccg aggtgtagta ggtgttgccg tcgttcttcg acgtggtcgt gtactggtcg
     3301 acgacctgca ccgcctgctg gtagttgtgg atctgcgtgt cgaagatgtc cgcgttcagg
     3361 atcaaggtct tgtccagcag cgcagacttc aggccgagct cgaacgagtt cgtcttctcc
     3421 gcagcggccg gcgttgacac gccgttgacc agctgcgcga tgccggcctt ctcgccatgc
     3481 tggtacgagg cgtagctcgt cacgtccggc gagatcttgt agctggggct caggacgagc
     3541 gcgggctggt tggcgcgaaa gggcttggct ccggtcgaat tgaacagcac gccgaggttg
     3601 gacgtgcgga tcgcctgggc cgccttgacc tgggccagct cggcggcggt caggctggcg
     3661 tacgtgcgcc ccgtgccgaa gtacttggcg gcggccgcgt cgcgagtggc cggatcgttg
     3721 aagttggcca gatcgacgcc gtcgcccggg ttgatgacgt acgagctgct gacgttggtg
     3781 cggttctcgt gcgtcaggcg cgcgccggtg gtcagcgtca ggcggtcggt gaagtgccag
     3841 ttcgcctgcg cgaagactgc gccgctcttg ttctcgatcg tctgctggcc ggcgggcgag
     3901 ttgtaggcca tcgacacgtt ggccagcgag tcgcgcatca gcagctggcc ggcggccgtg
     3961 ctgtcgagcg tgttgtactg cgccgtgctg gcgaaccagg cgccggcgtc agtgccccag
     4021 gcgcgctggt aggtcgacga gttgttgacc ttgatgaagt agaggccggc ctggtagtcc
     4081 aggaagccgc ccaccggcga gctgatgcgc agctcctggc tgaactggtg gtagtcgttc
     4141 cagaagccgc ctgcgttgcg gtagatgtcg aacggcgtgc cgtcgtcgtt gaccgcgttg
     4201 aagtggtagg acttgtaggc agtgatggaa gtcagcgtct ggccgctggg gatgcgccag
     4261 ttcacctcgg tggtcaagcc gttgcttccg gtgacgagcg cgtgctgcgc gtcgttgtcg
     4321 atgccggcgt agtagtcgtt gatgtcgtag ccggggttgt tggtgaacca actgcgcgcg
     4381 agccgggtcg agacgtcggt ggacagcgtg ttggtgaccc cgtcggcata ctgggtgggc
     4441 gtgggcgaga acttcgtgtt gccgttggtc gtctctccgg cgcgcggctg gacgtcgcct
     4501 tccaggcgcg cgctgaagct gtcgtcgggc ttgagcagga gctgcacgcg gcccgagacc
     4561 cggtccgtgt tggtgtaggt ctggtcccgg ttgtaggcgt tggcgcgcgc gccctgttgg
     4621 cgatcgaccg agaacgtgcc gcgccaggcg agcaggtcgt cgatcaccgg cccgccgatc
     4681 gcggcgacgc ccaggacgcc gttgttcttg ttgaaggcca gcgtgtagtc ggcggtcggc
     4741 gtgaacgacg gccgcttcgt attgatcgtg atgctgccga cgctcgcgtt cttgccctgc
     4801 agcgttccct gggggccgcg cgcgacctcg atggtgtcga tgtcgacgaa gtcgaagctg
     4861 gaggtcaggg cgttgtagcc gtagctgacg ccgtcgaaga tcacgccgac gctgggatcc
     4921 tgggcctcgg tctgcccgat cttgccgatg ccccggatcg acaggctgct cgtgcgctgg
     4981 ttgccgacgt tccaggagac gttggccgcg cgcttggcaa tcgcgcccac gccgtacgcg
     5041 tcgagtcgct cgagctcggc gccggtgacc accgacaccg acacgggcac atcctgcagg
     5101 cgctcgatgc ggttgcggct gtggacggtg accgcgtcca gggcgggggc ctcgttcgtg
     5161 gcatcggccg cgctggcggc gggcgccgcg gcggtggcgc tctgcgcctg ggcgttgccc
     5221 gacatcgtca tcgcgaggac ggcggcggcg acgggcagga gatggaaggg ggatctggcg
     5281 tgttgttgtt gttgttggcg ggaagtcgaa cggctcactg aggctcctcg gatgtcgttg
     5341 gaatggaaag ggcggatcga ggagctggaa tgcagcggcc atgccacgcg ggccggatat
     5401 gcaaatgcgg tggccgtgcg aaacgggtgg ggccccgcgt cgatccgacg cgcggcccgc
     5461 gcacgcggcg cgccgacggc cggacgcggc ttgctgcgtt cgcagcacgc ggcgccacgt
     5521 ggctgctcca aacgcagcgc atgctgcgcg tcttggcgcg ccgcggcgcg cgagcgccag
     5581 ggaatccgcg ttggcacgct tgctgcagcg agggcatgcg cgcatcgcgc cgccttcatc
     5641 gacacacacc aggaagcttc cgacatgacc actccccgat tcctcgtcgc cgcgctgctc
     5701 gccagcgcat ttgcctccgc ctcggcgcag gaggccgcgc cgcagcagcc ctggcgcgtc
     5761 gctggcggcg cgtccgaccc ggcggccgtc aagcgcgtgc aggagtcgct cgcggcaccg
     5821 tggaagtaca aggccaggcg cctcacgcgc gccgaggtcg acgcgctgct cgccaagccc
     5881 gaatcggtgg tcttcatcga cctgcgccgc cccgacgagt tcatcaccta cggcagcttc
     5941 ccggtgttca tcagcgtgca gaaccaggat ctcgagaagc agctggcctg gttgccgcat
     6001 gacaagacga tcgtcacggt gtccaaccat gcgcagcgcg ctggcgctgc cgccgacctg
     6061 ctcgctgcca agggcttcaa cgtggccggc gcgaccggtg ccgaggaata cgaggtgcag
     6121 ggcggcacga acgtcgcgca cgtcgcgccg ccggtgcgcc gcgccgcggc ggcggcgtcg
     6181 actccgcgat cctgagccga ggtcgcgatg agctcgcccg aactgatgtc ggatttcggc
     6241 gtcgccgagc ccgccggggc cgcgtgcgat gaggtgcacc gcgccgttct gtgggacctg
     6301 ccgcttcgcg tgttccactg gtcgctggtc gcggcggtga cggtcgcgat cgtcacgggc
     6361 gagctgggcg gcgcatggat gccgtggcac ggccgcgccg gcctcgtgat cgtcggcctg
     6421 ctggtgttcc gcctgctgtg gggcgtgctg ggttcggcca cctcgcgctt cacgcagttt
     6481 gccccgacgc ccgcgcgcct gcgcgcctac ctgcgtggac gctggcgcgg cgtcggccac
     6541 aacccgctgg gtgcgttgtc ggtgttcgcg ctgctggggc tgctgtcgct gcaggcggcc
     6601 accggcttgt tcggcaacga cgacatcgcg ttcgccgggc cgctgaacca tctggtcgac
     6661 gacgcggccg gcgcgcgcgc gaccggctgg catcgctggc tggccaacgg gctgatcgcg
     6721 ctgattgcgc tgcacctgct ggcgatcgcg ttccacgtgc tggtcaagcg ccatcgcctc
     6781 gtgcggccga tgatcaccgg gcggcacgcg ctggagccgg gtggcgactg gccgcggccg
     6841 cgcagctacg gcggctttgc gctgctgtcc gcggtggcgg tggcggcgac gtccgtgctc
     6901 gccttggcgg gtgcgggaga gacgccggtc gcgagcccgg cggcacccgc cagggccgcg
     6961 ctggtcgccg cgcccgcgca cgccgcgtcg acgcccgcct ggtgattcag tgaccgggcg
     7021 cgagccgctg cacggcggcc agcgcctgcg tcacgttctc gaccggcgac aggccgtcga
     7081 acgccgcggc gatgcggcca tcgggcgtca cgacgaaggt gctgcgctcg gccaggccgt
     7141 gctcgatctg ctggccgcgc gtgtccttgc gtccgggcgc ctcacgcacc gccagcgcgt
     7201 aggcgtgcgc gacgcggccg ccggtgtcgg acgcgacagg gatcttgccg gcgcaatagt
     7261 cggggtcggc ggagaatgcg ttgagccggc cgatgtcgtc gagcgacacg ccgatgatgg
     7321 acgccccggc ggccgcgaac tcggcctgcc gcgtcgcgaa cgtgtgggcc tccaggttgc
     7381 agccgccggt gtacgcggac gggtagaagt acacgacgac cgggccgcgc ttgaccgcgt
     7441 cggccagcga gaacgtgaac gtgcggccgg ccagcgatgc ctgcgccgtg aagggcgggg
     7501 cggcgtcgcc cgcggacaac gcggcgcgcg ccgcgggcat ggacagggcc agcgcggcga
     7561 gcgcgagcga ggaggcgagg gtgtgtttca tccgcgcgac cttgcaagcg ccgcgccagc
     7621 ggatgccgcg ccgccacgcg tcgacctgct gcgaacgaag cgatcggcag ccgcgcctgc
     7681 tgcgttcgac gcaatctcgt ctgcaaagcg cgcacgccgt cgtttcccgc atatcgacag
     7741 gcgaagtcgt tgcttggtgc ggcgcagcac tgcacttaac tttgcattcc cgccaacgag
     7801 attcacgaaa ccgtcatggc caagatcctg accctgccgg actcgcacac cgcgccgctg
     7861 accatccggg ccaaggcgct cgtgtttcac gacccgcagt cgcggctgct gctcgatcac
     7921 gtcgagcgcc tggcccgcag cgacgccacg gtgctggtga ccggcgagac cggcaccggc
     7981 aaggagctga tcgcgcgcca catccacgcc accagcggcc gcacggggcc gttcgtcgcc
     8041 gtcaactgcg gtgcgttcag cgagacgctg atcgacgccg agctgttcgg gcacgagacg
     8101 ggcgcgttca ccggcgcgac cacggcgcgc gccggctggt tcgaggcggc caacggcggc
     8161 actctgttcc tcgacgagat cggcgacctg ccgatggcgc tgcaggtcaa gctgctgcgc
     8221 gtgctgcagg agcgccaggt cgtgcgcctg ggctcgcggc ggccgatcgc gctcgacgtg
     8281 cggctggtgg ccgcgaccaa catcgacctc gcgcgcgccg tcacggcgca gcacttccgc
     8341 gccgacctgt actaccgcct gagcgtggcg ggcgtgcgcc tgccggcgct gcacgagcgc
     8401 caggccgaca tcctgccgct ggccgagcac ttcgtgggcg tgtactgcgc gcggcttggc
     8461 atccgcgcga tgccgttcag cgaggacgcc gtcgcggcgc tgcgttcgta cagctggccg
     8521 ggcaacatcc gcgagctgga gaacgtgatc cactacgcgc tgatcgtgtg cgccggcgac
     8581 gcgatccagg cgcaggacct gaagctggtg cccttgcagc cgccgtcggg gcgccgcgca
     8641 ggagacgcgt cgcctcggga cggccatccc ggattcggcg gctatcggcc ttcggaggag
     8701 cgcgagaggg cggcgccgcc ggcgcccgtg cccgcgagcg cgcgcgagcg gctgccggca
     8761 ttccttggcg agctgctgcg cgaaggcgga cccgagttgt tcgacagcgt cgagcgcgcg
     8821 ctggtgcatg ccgccttcga gcatgcgcac ggcaaccagg tgcgcgcggc gcgcgagctc
     8881 ggcgtcaccc gcaacatggt gcgcacgctg ctcaagcgcc atgggctgct cggctgcgag
     8941 ccgccgatcg atgcgagcct gtccgcggcc gaaggcgggc cggtcgcgct gtcggccgtg
     9001 taagcaacgg cgatttcgtt ctttgccttc gccgagctga attcccagaa tccattccat
     9061 cgatgagcga cgctggaggg cgtcgcgaac gccccgaacc gcaaaccaaa gaggaccttg
     9121 atgacgaccc cccgcctttc ccgaacgctc gtggccctgg cgctcgccgc caccgccgcc
     9181 gtctcgttcg ccaaggagat cacgctgctg aacgtgtcgt acgacccgac gcgcgagctc
     9241 taccaggaca tcgacgcgag cttcccgaag tactggaaga gcaagacggg cgacgacctc
     9301 aagatcaacg cgtcgcacgg cggctcgggc gcgcagtcgc gcgcggtgca ggacggcctg
     9361 gacgccgacg tcgtcacgct ggcgctggcc tccgacatcg acgccatcgc caacaagggc
     9421 ctcctggcca aggactggca gaagaagttc aaggacaaca gcacgcccta cacgtcgacg
     9481 atcgtgttcc tggtgcgcaa gggcaacccg aagggcatcc acgactggtc cgacatcgcc
     9541 aagcccgggg tgggcgtggt cgtggccaac ccgaagacct cgggcggcgc gcgctgggcc
     9601 tacctggcgg ccta
//
LOCUS       DATCRO010000601         5567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_749452, whole genome shotgun sequence.
ACCESSION   DATCRO010000601 DATCRO010000000
VERSION     DATCRO010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749452"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..460)
                     /locus_tag="VIN75_16390"
     CDS             complement(191..460)
                     /locus_tag="VIN75_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975801.1"
                     /translation="MDALNLFWHLANFVLPAVGVGALTAALCKLFWRKSLARTSWFTL
                     AWQAGAAGLAVLVAGLVITGHDGRMITWAGLVVACALVPWIRAGR"
     gene            549..1862
                     /gene="hemA"
                     /locus_tag="VIN75_16395"
     CDS             549..1862
                     /gene="hemA"
                     /locus_tag="VIN75_16395"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY8975802.1"
                     /translation="MSVFALGLNHQTAPIDLRGRFAFPLDQVSPALKALVQQFGRPGG
                     GAVALPEVALLSTCNRTEVYVADPTHKSSLVRPVADWLAAQGGVTGAQLLDHSYVLEN
                     RDAARHVFRVASGLDSMVLGEPQILGQMKQAVREADQAGTLGTTLHQLFQRSFSVAKE
                     VRTSTEIGAHSISMAAAAVRQATQLFGDLDKLNVLFVGAGEMIELVATHFAARTPKLM
                     AVANRTLERGEKLAVRLGAQAMRLADVPARLHEFDIVISCTASTLPIIGLGAVERALK
                     SRKRRPMFMVDLAVPRDIEPEVAQQNDVYLYTVDDLSELVRSAGEKRQAAVQQAEAII
                     DAGVLGFSHWLDQRATVPLIQALTRQADDWRSAELQRARKALAKGEPVDAVLEALSKG
                     LTQKMLHGTLAELRTSEGEQRHRLTDTVARLFLRQSTRDPADPGL"
     gene            1952..3073
                     /gene="prfA"
                     /locus_tag="VIN75_16400"
     CDS             1952..3073
                     /gene="prfA"
                     /locus_tag="VIN75_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8975803.1"
                     /translation="MKDSLRQQFDRLGMRLQELDARLADPAATHDMARYRELSREQSE
                     ASEIVELYRRFTQREADRAAAQEMLDDGDADMAAMAREEIAACDTDLQSLEAALQATL
                     LPRDPDDARPAFIEIRAGTGGEESALFAADLLRMYLRYAERQGWRSEVMSESASDLGG
                     YKEVVVRIENPSGAKGLAAATEGVYGQLRFESGGHRVQRVPATEAQGRIHTSACTVAV
                     LAEPDAAAEVTLNPSELRIDTFRASGAGGQHINKTDSAVRITHLPTGIVAECQDDRSQ
                     HRNKAKAMAVLLARLQDRERAEREAKDAAERKSLIGSGDRSDRIRTYNFPQGRLTDHR
                     INLTLYKLGAVMEGDLGEVVGALRAARAQEQLAALESGA"
     gene            3070..3921
                     /gene="prmC"
                     /locus_tag="VIN75_16405"
     CDS             3070..3921
                     /gene="prmC"
                     /locus_tag="VIN75_16405"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8975804.1"
                     /translation="MSLPGTVDAALREARARGLERLDAQLLLGHVLGQSRSWLMAHGD
                     DVLSAGHASAYASLVARRAAGEPFAYLVGEREFHGLTLAVAPAVLVPRPDTETLVDWA
                     LGLLQGELLDAPAPAVLDLGTGSGAIALAVKSGCPRALVWAGERSADALAVARGNAAR
                     LSLDVRFAQGDWWDALAGATDAPVFDLVVSNPPYIAADDPHLADLAHEPLSALVAADN
                     GLADIRAIATGARGRLRTGSWLLFEHGWEQADAVAAILAQAGFGRIATRRDIEGRPRC
                     TGGCLGA"
     gene            4123..4647
                     /locus_tag="VIN75_16410"
     CDS             4123..4647
                     /locus_tag="VIN75_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975805.1"
                     /translation="MEPSFSMTRRARAAALAILAATTLGVRAEGLVLRGYENPTSTHA
                     GSEYALTNSRLPTGSIRLLGDYYFVDPNQTSLGPATSSLVGGFRASTGVVSLAQPLSL
                     YETRPDPLQSLPYIGLGYSHLWFKSQLSLNADFGLASQTHGHGLLTSPSSLDDVTSQL
                     RWAPVMAVNVRYSF"
     gene            4746..5054
                     /gene="grxD"
                     /locus_tag="VIN75_16415"
     CDS             4746..5054
                     /gene="grxD"
                     /locus_tag="VIN75_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY8975806.1"
                     /translation="MSDVQQRIDDLVKNNKVVLFMKGTAQFPMCGFSGRAVQILKACG
                     VTELKTVNVLEDEEVRQGIKEYANWPTIPQLYVNGEFIGGSDILMEMYEAGELQPLLA
                     "
     gene            5051..>5567
                     /locus_tag="VIN75_16420"
     CDS             5051..>5567
                     /locus_tag="VIN75_16420"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY8975807.1"
                     /translation="MTSRVIVGITGASGAAYGVRLLERLRELGAETHLVVTPSGVINV
                     HHELGLSREDLAARATHTWPVGDIGACIASGSFAAQAMVVAPCTMKSLAAIAHGFGDN
                     LLMRAADVMLKERRRLVLMVRETPFNLAHLRNMTAVTEMGGVIFPPLPAFYHRPQNIA
                     EMVDHTVARVLD"
BASE COUNT          855 a         2065 c         1866 g          781 t
ORIGIN      
        1 tcaacaaggc cgccgcgcag gacgcgcaga acggcctgcc ggccaacaag accaacgccg
       61 tgtcctacgg cgcccaggtc gagatctggt tctgaggcga ggcgtcatcc tgcggcggcg
      121 tttcgtcgcg cgcaggatga cacccgatcg acgctggcgc ggcaaggcgc gcagccggcg
      181 tcggacaggc ctatcgaccg gcccggatcc agggcaccag cgcgcaggcg accaccagcc
      241 ccgcccaggt gatcatccgc ccgtcgtgcc cggtgatcac cagcccggcg accagcacgg
      301 ccagcccggc ggcgccggcc tgccaggcca gcgtgaacca gctcgtccgt gccaggctct
      361 tgcgccagaa cagcttgcac agcgcggccg tgagggcgcc caccccgacg gcgggcagca
      421 cgaagttggc gaggtgccag aacaggttca gggcgtccaa ggcggcgttt gatccggatc
      481 agaagggcgg tactgccggg aagaccagcg gtccccctgc ggcaacaggg aaattttata
      541 atgcgggcat gtctgtgttc gcgttgggac tcaaccacca gaccgcgccc atcgacctgc
      601 gcggtcgttt cgcgttcccg ctcgaccagg tttcccctgc gctcaaggcg ctggtgcagc
      661 agttcggccg tcccggcggc ggcgcggtcg ccctgcccga ggtggctctg ctgtccacct
      721 gcaaccgcac cgaggtctac gtcgccgacc cgacgcacaa gtcgtcgctc gtgcgcccgg
      781 tggccgactg gctggccgcc cagggcggcg tcacgggcgc ccagctgctc gaccacagct
      841 acgtgctgga aaaccgcgac gccgcgcgcc acgtgttccg cgtcgcctcc gggctcgatt
      901 ccatggtgct gggcgagccg cagatcctcg gccagatgaa acaggccgtg cgcgaggccg
      961 accaggccgg cacgctgggc accacgttgc accagctgtt ccagcgctcg ttctcggtgg
     1021 ccaaggaagt gcgcacgtcc accgagatcg gcgcgcactc gatcagcatg gccgccgcgg
     1081 ccgtgcgcca ggccacccag ctgttcggcg acctcgacaa gctcaacgtg ctgttcgtcg
     1141 gcgccggcga gatgatcgag ctggtcgcca cgcatttcgc ggcgcggacg cccaagctga
     1201 tggccgtggc caaccgcacg ctcgaacgcg gcgagaagct ggccgtgcgc cttggcgccc
     1261 aggccatgcg cctggccgac gtgcccgccc ggctgcacga gttcgacatc gtcatctcct
     1321 gcaccgccag caccctgccc atcatcggcc tgggcgccgt cgagcgcgcg ctcaagagcc
     1381 gcaaacgccg cccgatgttc atggtcgacc tggccgtgcc gcgcgacatc gagcccgagg
     1441 tcgcgcagca gaacgacgtc tacctgtaca cggtcgacga cctgtccgag ctggtgcgca
     1501 gcgccggcga gaaacgccag gcggccgtcc agcaggccga ggccatcatc gacgccggcg
     1561 tgctgggctt ctcgcactgg ctcgaccagc gcgccaccgt gccgctgatc caggcgctca
     1621 cccgccaggc cgacgactgg cgcagcgccg agctccagcg cgcgcgcaag gcgctggcca
     1681 agggcgagcc ggtcgacgcg gtgctggagg cgctcagcaa gggcctgacg cagaagatgc
     1741 tgcacggcac gctcgccgag ctgcgcacca gcgagggcga acagcgccat cggctcacgg
     1801 acaccgtcgc gcggctgttc ctgcgccagt ccacgcgcga cccggccgac cccggcctct
     1861 agcgacgccc gtcgtcgcgg cgccgtcgac ggcgccggat cgccagcccg cgcgtcgtgg
     1921 gctgcccgcc tgctgtcccg tccggatccc catgaaagac tcgctccgcc aacaattcga
     1981 ccgcctgggc atgcgcctgc aggagctcga cgcgcgcctg gccgacccgg ccgccaccca
     2041 cgacatggcc cggtaccgcg agctctcgcg cgagcagtcc gaggcctcgg agatcgtcga
     2101 gctgtaccgc cgcttcaccc agcgcgaggc cgatcgcgcc gccgcgcagg agatgctgga
     2161 cgacggcgac gccgacatgg ccgcgatggc gcgcgaggag atcgccgcct gcgacaccga
     2221 tctccaatcg ctggaagccg cgctgcaggc gacgctgctg ccgcgcgacc cggacgatgc
     2281 gcgtcccgcc ttcatcgaga tccgcgccgg caccggcggc gaggaatcgg cgctgttcgc
     2341 cgccgacctg ctgcgcatgt acctgcgcta tgccgagcgc cagggctggc gcagcgaggt
     2401 catgagcgag agcgcgtccg acctcggcgg ctacaaggaa gtcgtcgtgc gcatcgagaa
     2461 cccgtcgggc gccaagggcc tggccgcggc caccgagggc gtgtacggcc agctgcgctt
     2521 cgagtcgggc ggccaccgcg tgcagcgcgt gcccgccacc gaggcacagg gccgcatcca
     2581 caccagcgcc tgcaccgtgg ccgtgctggc cgagcccgac gccgcggccg aggtcacgct
     2641 caacccgtcc gagctgcgca tcgacacctt ccgcgccagc ggcgccggcg gccagcacat
     2701 caacaagacg gacagcgccg tgcgcatcac gcacctgccc accggcatcg tcgccgaatg
     2761 ccaggacgac cgctcgcagc accgcaacaa ggccaaggcg atggccgtgc tgctcgcgcg
     2821 gctgcaggat cgcgagcgcg ccgagcgcga ggccaaggac gccgccgagc gcaagagcct
     2881 gatcggctcg ggcgatcgtt ccgaccgcat ccgcacctac aacttcccgc agggccgcct
     2941 gaccgaccac cgcatcaacc tgacgctcta caagctgggc gccgtgatgg aaggggacct
     3001 cggcgaggtc gtgggcgccc tgcgcgccgc gcgcgcccag gagcagctcg ccgcgctgga
     3061 gtcgggcgcg tgagcctgcc cggcaccgtc gacgccgcgt tgcgcgaggc ccgcgcccgc
     3121 ggcctcgagc ggctcgacgc gcagctgctg ctgggccacg tgctggggca gtcgcgcagc
     3181 tggctgatgg cgcacggcga cgacgtcctg agcgccggcc acgcgtcggc ctacgcatcg
     3241 cttgtcgcac gccgcgcggc cggcgagccc ttcgcctacc tggtcggcga acgcgagttc
     3301 cacggcctga cgctggccgt cgcccccgcc gtgctggtgc cgcggcccga caccgagacg
     3361 ctggtcgact gggcgctcgg cctgctgcaa ggcgaactgc tcgatgcgcc ggcccccgcc
     3421 gtgctcgacc tgggcaccgg cagcggcgcg atcgcgctgg ccgtcaagag cggctgccca
     3481 cgcgccctcg tgtgggcggg cgagcgcagc gcggacgcgc tcgcggtcgc gcgcgggaac
     3541 gccgcccggc tgtccctgga cgtgcgtttc gctcaaggcg actggtggga cgcgctggcc
     3601 ggcgcgacgg acgcgcctgt cttcgacctc gtcgtgagca acccgcccta catcgccgcc
     3661 gacgacccgc acctggcgga cctggcccac gagccgctgt ccgcgctcgt ggctgccgac
     3721 aacggcctgg ccgacatccg ggccatcgcc acgggcgccc gcggccggct gcgcacgggc
     3781 agctggctgc tgttcgagca cggctgggag caggcggacg ccgtcgcggc gatcctcgcc
     3841 caggcgggtt ttggtcgtat cgcaacgcgg cgcgacatcg agggtcgccc tcgctgcacc
     3901 ggcggctgtc tgggcgctta actcaccggt tttagggcat cgcggccgcg cacgcaaggg
     3961 cccgaatcgc gctatcgtga catcgtctct gggtcgtcac aggaggtaat caagcctgga
     4021 gaaagcgcgc tgtttgtcga tacatccaac acgacaacag gcgtagcatg gattttctgg
     4081 agtctggttt ttctgccccg ccgggcgatt cgggagccga ccatggaacc ttcattttcg
     4141 atgacgcgtc gcgcgcgggc cgccgccctc gcgatcctcg cggccaccac cctgggtgtt
     4201 cgagctgaag gcctggtctt gcgaggatac gagaacccga caagcaccca tgcgggctcc
     4261 gagtatgcac tcacaaactc ccgcctgccg accggctcga tccgcctgct gggcgactac
     4321 tacttcgtcg atcccaacca gaccagtctc ggcccggcca cgagcagcct cgtcggcggc
     4381 ttccgcgcca gcaccggcgt ggtcagcctg gcgcagccgc tgtcgctgta cgagacccgt
     4441 cccgatccgc tgcagagcct gccctacatc ggcctgggct acagccacct ctggttcaag
     4501 agccagctga gcctgaacgc cgacttcggc ctcgcctcgc agacccacgg ccacgggctg
     4561 ctcaccagcc ccagcagcct cgacgacgtc accagccagc tgcgctgggc gccggtgatg
     4621 gccgtcaacg tccggtattc gttctaaggc acgttgcggc gcgacctttg gtgtgcgatg
     4681 actatccgtt tggcttataa acgggagatc ggcgccgcgt gcgccttctc ctgatcccga
     4741 ctgccatgtc cgacgtccag caacgcatcg atgacctcgt gaagaacaac aaggtggtgt
     4801 tgttcatgaa gggcaccgcc cagttcccca tgtgcggctt ctccggccgc gccgtccaga
     4861 tcctgaaggc ctgcggcgtg accgagctga agacggtcaa cgtgctggag gacgaggaag
     4921 tgcgccaggg catcaaggaa tacgccaact ggccgaccat cccgcagctg tacgtcaacg
     4981 gcgagttcat cggcggctcg gacatcctga tggagatgta cgaagcgggc gagctgcagc
     5041 cgctgctggc ttgacctcgc gggtcatcgt cggcatcacc ggcgcctcgg gcgccgccta
     5101 cggcgtgcgc ctgctggaac gcctgcgcga gctgggcgcc gagacgcacc tggtcgtcac
     5161 gccctcgggc gtcatcaacg tccaccacga gctggggctg tcgcgcgagg acctcgccgc
     5221 cagggccacg cacacgtggc ccgtgggcga catcggcgcc tgcatcgcca gcggcagctt
     5281 cgccgcgcag gcgatggtgg tggcgccgtg cacgatgaag tcgctcgcgg ccatcgccca
     5341 cggcttcggc gacaacctgc tcatgcgtgc ggccgacgtc atgctcaagg agcgccgccg
     5401 gctggtgctg atggtgcgcg agacgccgtt caaccttgcc cacctgcgca acatgaccgc
     5461 cgtcaccgag atgggcggcg tcatcttccc tcccctgccc gcgttctatc atcgacctca
     5521 aaacattgcg gagatggtcg accataccgt cgcgcgcgta ctggatc
//
LOCUS       DATCRO010000602         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_962919, whole genome shotgun sequence.
ACCESSION   DATCRO010000602 DATCRO010000000
VERSION     DATCRO010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962919"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="pstC"
                     /locus_tag="VIN75_16425"
     CDS             complement(<1..913)
                     /gene="pstC"
                     /locus_tag="VIN75_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8975808.1"
                     /translation="MPWADTLFAVLAHGAAWLTLAMLAGIIGSLVAGAMPALRAYGPA
                     FLWTSEWDPVQDHYGGLAMIYGTLATSLIALVIAVPVSFGIALFLTEMSPSWLKRPLG
                     TAIELLAGVPSIVYGMWGLTVFGPILASWVQQPLQSAFGNVPGLSTLFAGPPVGIGIL
                     CAGIILAIMIIPFIASVMRDVFEVTPPMLKESAYGLGATTWEVVYKVVLPFTKTGVVG
                     GIMLGLGRALGETMAVTFVIGNTNQLNSLSVFEAANSITSVLANEFGEAEGMHRAALL
                     YLALVLFVITFIVLSLAKVLLMRLQRGE"
     gene            complement(1081..2124)
                     /gene="pstS"
                     /locus_tag="VIN75_16430"
     CDS             complement(1081..2124)
                     /gene="pstS"
                     /locus_tag="VIN75_16430"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY8975809.1"
                     /translation="MKFQPIRLVTIAAALSVAGVAAQAADEVTGAGSSFAAPIYSKWA
                     EAYAKASSNKVNYQSVGSGAGIKQIKAKTVDFGASDMPLKDEELKEAGLVQFPTCMGG
                     VVPVINVAGVKPGQLKLTGTVLADIYAGKVTKWNDAEITGLNPGVTLPDAQIATVHRA
                     DGSGTSFIFTNYLSKVNAEWKSKVGEGTAVNWPGNGVGGKGNEGVAQYVQRLPNSIGY
                     VEYAYAKQNKMDYVLLKNSAGSFVAPDDATFKAAAAGADWSKTYYQVLTDEPGKDSWP
                     ITGATFVLLQKVQDKPAQGAAVMKFFDWAFANGDKAAAELDYVSLPDALKAQIRKTWG
                     DVKDGSGKTVAFK"
     gene            complement(2323..>3081)
                     /gene="glmM"
                     /locus_tag="VIN75_16435"
     CDS             complement(2323..>3081)
                     /gene="glmM"
                     /locus_tag="VIN75_16435"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067335.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8975810.1"
                     /translation="VFHELGADVACIACSPDGLNINDQVGATHPEALVAAVKAQGANF
                     GIALDGDADRLQMVDASGRCYDGDELLYAVVADRLSRGQPVPGVVGTLMTNMAVEQAL
                     RGRGVDLVRAKVGDRYVLEELLARGWQLGGENSGHLLVLDRHTTGDGIVSALQVLQAV
                     SRTGQSLQALLDGVRLFPQVMINVRLAVRSDWQKNATLVAEKAAVERELGADGRVLIR
                     ASGTEPVLRVMVEARDKAVARGFAERLAEAAKAA"
BASE COUNT          484 a         1049 c         1058 g          490 t
ORIGIN      
        1 cttcgccgcg ttgcaggcgc atcagcagca ccttggcgag cgacagcacg atgaaggtga
       61 tgacgaacag caccagcgcg aggtacagca gcgccgcgcg gtgcatgccc tcggcctcgc
      121 cgaactcgtt ggccagcacc gaggtgatgc tgttggcggc ctcgaagacc gacagggaat
      181 tgagctggtt ggtgttgccg atcacgaagg tgacggccat cgtctcgccg agcgcgcggc
      241 ccagcccgag catgatgccg ccgacgacgc cggtcttggt gaacggcagc acgaccttgt
      301 agacgacctc ccaggtcgtc gcgcccaggc cgtaggccga ttccttcagc atcgggggcg
      361 tgacctcgaa gacgtcacgc atcacggaag cgatgaaggg gatgatcatg atggccagga
      421 tgatcccggc gcacaggatg ccgatgccca cgggcggccc cgcgaacagc gtcgacagcc
      481 ccggcacgtt gccgaacgcc gactgcaggg gctgctgcac ccagctggcg aggatggggc
      541 cgaagacggt caggccccac atgccgtaga cgatggacgg cacgccggcg agcagttcga
      601 tggcggtgcc cagcgggcgc ttcagccacg acggcgacat ctcggtcagg aacagcgcga
      661 tgccgaagct caccggcacg gcgatcacca gcgcgatcag cgaggtggcc agcgtgccgt
      721 agatcatcgc caggccgccg tagtggtcct gcacgggatc ccactcgctg gtccacagga
      781 acgcgggccc gtaggcgcgc agcgcgggca tcgcgcccgc caccagcgaa ccgatgatgc
      841 ctgccagcat cgccagcgtc agccacgccg cgccgtgggc gaggacggcg aacaaggtgt
      901 cggcccaggg cagggtctgg cgccgctcgg gcggccgacc ggccggctgg gcgctgtcct
      961 tcgcgttgcg cagggtggtt tcggcgggca gtgtggcagc cacgagggcc tccggttcga
     1021 ggagttttca ggccgccggc cggaggcccg gcgcggcggc aggatcgccc aggcgagcga
     1081 ttacttgaag gcgaccgtct tgccggagcc gtccttcacg tcgccccagg tcttgcggat
     1141 ctgggccttc agcgcgtcgg gcagcgagac gtagtcgagt tcggcggcgg ccttgtcgcc
     1201 attggcgaac gcccagtcga agaacttcat gaccgcggcg ccctgggccg gcttgtcctg
     1261 caccttctgc agcagcacga aggtcgcgcc ggtgatgggc cacgaatcct tgcccggctc
     1321 gtcggtcagg acctggtagt aggtcttgga ccagtcggcg cccgcggcgg cggccttgaa
     1381 cgtggcgtcg tccggagcga cgaagctgcc tgccgagttc ttcaacagga cgtagtccat
     1441 cttgttctgc ttggcatagg catattcgac gtagccgatc gagttcggca ggcgctgcac
     1501 gtactgcgcg acgccttcgt tgcccttgcc gccgacgccg ttgcccggcc agttcacggc
     1561 ggtgccttca ccgaccttgg acttccattc cgcgttcacc ttcgacaggt agttcgtgaa
     1621 gatgaagctg gtgcccgagc cgtcggcgcg gtgcacggtg gcgatctgcg cgtccggcag
     1681 cgtcacgccg gggttcaggc cggtgatctc cgcatcgttc cacttggtga ccttgccggc
     1741 gtagatgtcg gcgagcacgg tgcccgtcag cttcagctgg cccggcttga cgccggccac
     1801 gttgatgacc ggcacgacgc cgcccatgca ggtggggaat tgcacgaggc cggcttcctt
     1861 gagctcctcg tccttcagcg gcatgtcgga agcgccgaag tcgaccgtct tggccttgat
     1921 ctgcttgatg ccggcgcccg agcccaccga ctggtagttg accttgttgc tggaggcctt
     1981 cgcataggcc tcggcccact tcgagtagat cggggcggcg aacgacgaac cggcgccggt
     2041 gacttcgtcg gctgcctggg cggcgacgcc ggccacgctc agggcggcgg cgatggtcac
     2101 caggcgaatc ggttggaact tcatgatcgg gcctttcagg ggcagtttaa tgggactgcg
     2161 ccaatgtaga aggccgccat gacaggactg tgacacaacc gtggcaggtc gccgcacagc
     2221 cttgcccgag ggaaaatgcc ctgtcgaatc agtaccttgc gaccgccggg cggcgctcgc
     2281 cgccggattg tgacaaccgc ggatgcgact gtcacatcct tgtcacgccg ccttggcggc
     2341 ctcggccagg cgctccgcga agccccgcgc gaccgccttg tcgcgcgcct ccaccatcac
     2401 gcgcagcacc ggctcagtgc ccgaggcgcg gatcagcacg cggccatcgg cgcccagttc
     2461 gcgttcgacc gccgccttct cggcgaccag cgtcgcgttc ttctgccaat cggaccggac
     2521 ggccaggcgc acgttgatca tcacctgcgg gaacaggcgc acgccgtcga gcagcgcctg
     2581 cagcgactgg ccggtgcggc tgaccgcctg caatacctgc agcgcgctga cgatgccgtc
     2641 gccggtggtg tggcggtcga gcacgagcag gtggcccgag ttctcgccgc ccagctgcca
     2701 gccacgagcc agcagttcct cgagcacgta gcggtcgccg accttggcgc gcacgaggtc
     2761 gacgccgcgg ccgcgcagcg cctgctcgac cgccatgttg gtcatcagcg tgccgacgac
     2821 gccaggtacc ggctggccgc gcgacagccg gtcggcgacg accgcgtaca gcagctcgtc
     2881 gccgtcgtag caacggccgc tcgcatcgac catctgcagg cggtcggcgt cgccgtccag
     2941 cgcgatgccg aagttcgccc cttgcgcctt cacggccgcg acaagcgcct cggggtgcgt
     3001 cgcaccgacc tggtcgttga tgttcaggcc gtccggagag cacgcgatgc acgcgacgtc
     3061 cgcgccgagc tcatggaaca c
//
LOCUS       DATCRO010000603         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_816648, whole genome shotgun sequence.
ACCESSION   DATCRO010000603 DATCRO010000000
VERSION     DATCRO010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816648"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIN75_16440"
     CDS             complement(<1..432)
                     /locus_tag="VIN75_16440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY8975811.1"
                     /translation="MLTFQQIILRLQDYWDKQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPDNILELYLGSLEALGF
                     DLKKNDVRFVEDDWENPTLGCWGLGWEVWLNGMEVTQFTYFQ"
     gene            complement(536..1018)
                     /gene="bamE"
                     /locus_tag="VIN75_16445"
     CDS             complement(536..1018)
                     /gene="bamE"
                     /locus_tag="VIN75_16445"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY8975812.1"
                     /translation="MLVLSLVALPGCAAHPFAPTSVPAGAPRDDVLRIMGPPTATYVM
                     PDGHERLEYNRMPFGRHTFMIDLDAAGRVAHWEDVLDEAHFAAIRPGMTRADVLRLIG
                     PPSFTSNYHRPEPGFTWNYGFQAPLRCIVFQVPFSEATGRVVEPGGYPSDPRCADEWM
                     "
     gene            complement(1063..>2533)
                     /gene="lnt"
                     /locus_tag="VIN75_16450"
     CDS             complement(1063..>2533)
                     /gene="lnt"
                     /locus_tag="VIN75_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8975813.1"
                     /translation="ALSFAPAELWWLQVIATGALVALLADAPPRAAAARAWAFAFGWL
                     AAGFWWLYISLHVYGFLPAWLSALAVAVLAALLALYYAIAGWAWARLRRNRPLVDAVL
                     FSSVWLMAELARGQFLTGFPWIAGGYAHTSGPLARWAPYVGVYGIGALAAFVSAAVAL
                     VLMAQRGPRTRAPLVTLSPLLVPLAALLVGLALPQAFTRSTGILTVSLLQPDVAQNDK
                     FEEEHVRQALAWHVVKLASAPGQLVVTPESSLPILPVQIPRETWLAYEKPFNRTGRSA
                     LVGVFTGDDEHGYTNSLVGLDTMHHMTDGTFYRYGKRHLLPFGEYVPPGFHWFVSMMK
                     IPIGDQAAGVDTASFQVGAQRVRPLICYEDLFGEDFAPSMVGPNAATLMANSTNLAWF
                     GRRMIQDQHLQFSRMRALEFQRGQVRATNTGATAVIDWHGHVTARLPADVEGELDAQV
                     EGRIGDTPYGIWVARLRLWPLWGLALAQLLFGVFMARRR"
BASE COUNT          404 a          870 c          934 g          325 t
ORIGIN      
        1 ctggaagtag gtgaactgcg tgacttccat gccgttgagc cagacttccc agcccaggcc
       61 ccagcagccg agcgtcgggt tctcccagtc gtcctcgacg aagcgcacgt cgttcttctt
      121 caggtcgaag cccagcgcct cgagcgagcc gagatacagc tcgaggatgt tgtcgggcgc
      181 cggcttcagg acgacctggt actggtagta gtgctgcagg cggttcgggt tctcgccgta
      241 gcggccgtcc ttggggcggc ggctgggctg cacgtaggcg gccttccacg gctcggggcc
      301 gagggcgcgc aggaaggtgg cggtgtggct ggtgccggcg ccgacttcca tgtcgtaggg
      361 ctgcagcagc gcgcagccct gcttgtccca gtagtcctgc aggcgaagaa tgatttgctg
      421 gaaggtcagc atgggtgaat tcggtggggc cgcggcggcc ggaggcgaca ggccgcgagc
      481 gggggccgcg cgcggtcgaa cggacgattt tacgtgcggc gcgaccgatc gcgggtcaca
      541 tccattcatc cgcgcaacgc gggtcggacg gatagccgcc cggctcgacc acccggcccg
      601 tcgcctcgct gaacggaact tggaacacga tgcagcgcag cggagcctgg aagccgtagt
      661 tccaggtgaa gccgggctcc gggcggtggt agttcgacgt gaacgacggc ggcccgatca
      721 ggcgcagcac gtcggcccgg gtcatgccgg gccggatcgc cgcgaagtgg gcttcgtcga
      781 gcacgtcctc ccagtgcgcc acgcggcccg cggcgtcgag gtcgatcatg aaggtgtgcc
      841 ggccgaacgg catgcggttg tattcgagcc gctcgtggcc gtcgggcatc acgtaggtgg
      901 ccgtgggcgg gcccatgatg cgcagcacgt cgtcgcgcgg cgcgcccgcg ggcacggacg
      961 tgggcgcgaa cgggtgcgcc gcgcaaccgg gcagggccac cagggacagg acgagcaagg
     1021 cactgcgggt cgacatcccg caaggatgcc agacccgcga agtcaacgcc ggcgcgccat
     1081 gaacacgccg aacagcagct gcgcgagcgc caggccccac agcggccaca gccgcagcct
     1141 ggccacccag atgccgtagg gcgtgtcgcc gatgcggccc tcgacctggg cgtcgagctc
     1201 gccctcgacg tccgccggca ggcgcgcggt gacgtggccg tgccagtcga tgacggcggt
     1261 ggcgccggtg ttggtggcgc gcacctgtcc gcgctggaac tcgagcgcgc gcatgcgcga
     1321 gaactgcagg tgctggtcct ggatcatgcg gcggccgaac caggccaggt tcgtggagtt
     1381 ggccatcagc gtggccgcgt tcgggcccac catggacggc gcgaagtcct cgccgaacag
     1441 atcctcgtag cagatcagcg gccgcacgcg ctgcgcgccc acctggaacg acgcggtgtc
     1501 gacgcccgcg gcctggtcgc cgatcgggat cttcatcatc gagacgaacc agtggaagcc
     1561 cggcggcacg tactcgccga acggcagcag gtggcgcttg ccgtagcggt agaacgtgcc
     1621 gtcggtcatg tggtgcatgg tgtccaggcc gaccagcgag ttcgtgtagc cgtgctcgtc
     1681 gtccccggtg aaaacgccca ccagcgccga gcggcccgtc ctgttgaacg gcttctcgta
     1741 ggccagccag gtctcgcgcg ggatctgcac cggcaggatc ggcagggacg actcgggcgt
     1801 caccaccagt tgcccgggcg cgctggccag cttgacgacg tgccaggcga gcgcctgccg
     1861 gacgtgctcc tcctcgaact tgtcgttctg cgccacgtcc ggctgcagca gcgacaccgt
     1921 gaggatgccg gtcgagcggg tgaatgcctg cggcagcgcg aggccgacca gcagcgccgc
     1981 cagcggcacc agcagcgggc tcagcgtgac caggggcgcg cgcgtgcgcg gcccgcgctg
     2041 cgccatcagc accagcgcca ccgcggcaga gacgaacgcc gccagcgcgc cgatgccgta
     2101 cacgccgaca tacggcgccc accgggcgag cggcccggac gtgtgcgcat agccgccggc
     2161 gatccacggg aagccggtca ggaactgccc gcgcgccagt tcggccatca gccagaccga
     2221 cgagaacagc accgcgtcga ccaggggccg gttgcgccgc agccgcgccc acgcccagcc
     2281 ggcgatcgcg tagtacagcg ccagcagcgc cgccagcacc gccacggcca gcgccgacag
     2341 ccaggccggc aggaagccgt agacgtgcag gctgatgtag agccaccaga agccggcggc
     2401 cagccagccg aaggcgaagg cccaggcgcg cgccgcggcc gcgcgcgggg gggcgtcggc
     2461 cagcagcgcg accagcgcgc cggtggcgat gacctgcagc caccagagct cggccggtgc
     2521 gaaggacagc gcc
//
LOCUS       DATCRO010000604         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_186919, whole genome shotgun sequence.
ACCESSION   DATCRO010000604 DATCRO010000000
VERSION     DATCRO010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186919"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VIN75_16455"
     CDS             <1..595
                     /locus_tag="VIN75_16455"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY8975814.1"
                     /translation="ARRCIHSRHCVLEAPMVFRANTPGDWITPDALDQEALVAVAERC
                     PSGAIRYRRRDGRPGESAPPVNTLHVRENGPYALRAPLSVAGVDDGLRATLCRCGRSR
                     NKPWCDGSHVAAGFAATGEPPTGAIDVLAGRDGPLAVTPLRDGPLQVRGNLEICAGTG
                     RTVARVTQARLCRCGHSRDKPFCDLSHVAAGFVAAGE"
     gene            complement(631..1272)
                     /locus_tag="VIN75_16460"
     CDS             complement(631..1272)
                     /locus_tag="VIN75_16460"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY8975815.1"
                     /translation="MSAPPLLPARFEDWCDPRRTALLVYDMQVGVCRQVARSAAVIER
                     CGVALQAARRAGMRIAFTRHRSPPRAWLGATQLRTAMAWQRASSPDDVQSWFARGAPA
                     AEIVPELAPRDDELVVDKFAMSAFEGTPLATAWRDGGLTGVAIAGIALEIGIEPTVRH
                     ATDLGLVPIVLADACGAGHAEAGERTLATMRFVGEAVFGDVDGFAAALARTPT"
     gene            complement(1269..1601)
                     /locus_tag="VIN75_16465"
     CDS             complement(1269..1601)
                     /locus_tag="VIN75_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY8975816.1"
                     /translation="MKIIAFDNFKPGVTLDDLRPYLRDEVANVWRLWKAGIVRENFAR
                     TDVGGVVIVFECSDIAEARRHIDDFPLTRAGLIEWQLIGCGAPLPLEFLFDPAVDVAE
                     PLDRTGRH"
     gene            1686..2045
                     /locus_tag="VIN75_16470"
     CDS             1686..2045
                     /locus_tag="VIN75_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8975817.1"
                     /translation="MKRLPRTFGCPTELALELLGGKWKTVILARLKEGPQRYGELRRQ
                     IPRLSDKMLTQCLHGLQERGLVARDAAPDSTARYRLTPAGRSLRPLLQQLYDWGDAQV
                     ERLGVTIRDRAGEPVDA"
     gene            complement(2055..>2585)
                     /locus_tag="VIN75_16475"
     CDS             complement(2055..>2585)
                     /locus_tag="VIN75_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8975818.1"
                     /translation="ALSRTAERALAPFAVVLQVTPIVAIAPLIMILTDSPLATVIVCA
                     WLVAFFPILANTLTGLRSVDANLKDLFALQRASRWQTLRHLLVPAALPSFMTGVRIAG
                     GLSLIGEVGGEFAAGAAGRDTGLASRILESAFRTEIPKMFAALALVAATGVALYLATG
                     ALSRLLLQRWHESERS"
BASE COUNT          362 a          940 c          973 g          310 t
ORIGIN      
        1 ggcgcgccgc tgcatccact cgcgccactg cgtgctggag gcaccgatgg tgttccgcgc
       61 caacacgccg ggcgactgga tcacgccgga tgcgctcgac caggaggcgc tggtcgccgt
      121 cgccgagcgc tgcccgtcgg gcgcgatccg ctaccgccgc cgcgatggcc ggcccggcga
      181 atccgcgccg ccggtcaata cgctgcacgt gcgcgagaac gggccctacg ccctgcgcgc
      241 gccgctcagc gtggccggcg tcgacgacgg gctgcgagcg acgctgtgcc gctgcggccg
      301 gtcgcgcaac aagccctggt gcgacggcag ccatgtcgcg gcgggcttcg cggcgacggg
      361 cgagccgccg accggcgcga tcgacgtgct cgccgggcgc gacggcccgc tcgcggtcac
      421 gcccctgcgc gacggcccgc tgcaggtgcg tggcaacctg gagatctgcg cgggcaccgg
      481 ccgcaccgtc gcgcgcgtga cgcaggcgcg cctgtgccgc tgcgggcact cgcgcgacaa
      541 gccgttctgc gacctgtcgc acgtggcggc cggcttcgtc gcggccggcg agtgagcgat
      601 gcggcctcgc gcgcggcgag gccgccgccg tcacgtgggc gtgcgggcga gcgccgcggc
      661 gaagccgtcg acgtcgccga agacggcctc gccgacgaag cgcatcgtcg ccagggtgcg
      721 ctcgccggcc tcggcgtggc ccgcgccgca ggcgtcggcc agcacgatgg gcacgaggcc
      781 gaggtcggtc gcgtggcgca cggtcggctc gatgccgatc tccagcgcga tgcccgcgat
      841 cgcgacgccc gtgaggccgc cgtcgcgcca cgcggtggcc agcggcgtgc cctcgaacgc
      901 agacatggcg aacttgtcga cgaccagttc gtcgtctcgc ggcgcgagct cgggcacgat
      961 ctccgcggcc ggagcgccgc gggcgaacca cgactgcacg tcatcgggcg agctcgcgcg
     1021 ctgccaggcc atcgcggtgc gcagctgcgt cgcgcccagc cacgcccgcg gcggtgaccg
     1081 gtggcgcgtg aacgcgatgc gcatgccggc tcgccgcgcg gcctgcaacg cgacgccgca
     1141 gcgttcgatg accgcggccg accgcgccac ctggcggcag acacccacct gcatgtcgta
     1201 gaccagcagc gcggtgcggc gcgggtcgca ccagtcctcg aagcgcgcgg gcaggagcgg
     1261 aggcgcgctc aatgccggcc cgtccggtcc aagggctcgg cgacgtcgac tgccgggtcg
     1321 aacaggaact cgagcggcag cggcgcgccg cagccgatga gctgccactc gatgaggccg
     1381 gcgcgcgtca gggggaagtc gtcgatgtgg cgccgcgcct cggcgatgtc gctacattcg
     1441 aagacgatga cgacgccgcc gacgtcggtg cgcgcgaagt tctcgcgcac gatgccggct
     1501 ttccacaggc gccacacgtt ggcgacttcg tcgcgcagat agggccgcag gtcgtccagc
     1561 gtgacgccgg gcttgaagtt gtcgaaggca atgatcttca tggagaactc gatgaacaag
     1621 gacgcctgca ggctacgggc cgccccggcg agggtcaacg caccgcttgg tgcgttggac
     1681 gcgcgatgaa gcgccttccc cgcacgttcg gctgtccgac cgagctcgcg ctggaactgc
     1741 tgggcggcaa gtggaagacg gtgatcctgg cgcgcctgaa ggaaggcccg cagcgctacg
     1801 gcgaactgcg gcgccagatc ccgcgcctga gcgacaagat gctcacgcag tgcctgcacg
     1861 gcctgcagga gcgcgggctg gtggcgcgcg acgccgcgcc ggactcgacc gcgcgctacc
     1921 gcctgacgcc cgcgggccgc tcgctgcggc cgctgctcca gcagctgtac gactggggcg
     1981 atgcgcaggt ggagcggctg ggcgtcacca tccgcgaccg cgccggcgag ccggtcgacg
     2041 cctgacgcgc cgcttcagct gcgctcgctc tcgtgccagc gctgcagcag cagccggctc
     2101 agcgcacccg tggccaggta cagcgccacg cccgtggcgg cgacgagcgc gagggccgcg
     2161 aacatcttgg ggatctcggt gcggaacgcc gactcgagga tgcgcgaggc caggcccgtg
     2221 tcgcggcccg cggcgcccgc ggcgaactcg ccacccacct cgccgatcag cgacagtccg
     2281 ccggcgatgc gcacgcccgt catgaacgag ggcagcgcgg cgggcaccag caggtggcgc
     2341 agcgtctgcc agcggctcgc gcgctgcagc gcgaacaggt ccttcaggtt cgcgtcgacg
     2401 ctgcgcaggc cggtgagcgt gttggccagg atggggaaga acgccaccag ccaggcgcac
     2461 acgatcacgg tggcgagcgg gctgtcggtc aggatcatga tcagcggcgc gatggccacg
     2521 atgggcgtga cctgcagcac caccgcgaat ggcgccagcg cgcgctcggc ggtgcgcgac
     2581 agcgc
//
LOCUS       DATCRO010000605         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_242946, whole genome shotgun sequence.
ACCESSION   DATCRO010000605 DATCRO010000000
VERSION     DATCRO010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242946"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VIN75_16480"
     CDS             <1..1165
                     /locus_tag="VIN75_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8975819.1"
                     /translation="QEIVERDLYATAVLSGNRNFDGRIHPHAKQAFLASPPLVVAYAI
                     AGTIRFDIERDVLGVAGDGREIRLADLWPADEEIDAIVAASVRPEMFRKVYEPMFAIH
                     ADRGEKVSPQYDWRPQSTYIRRPPYWDREGVGALAANPRTLRGMRPLALLPDNITTDH
                     LSPSNAILPDSAAGEYLARMGLPEEDFNSYATHRGDHLTAMRATFANPQLVNEMAVVD
                     GVQRKGSLARIEPEGRVVRMWEAIEVYLERRQPLIIVAGADYGQGSSRDWAAKGVRLA
                     GVEAIAAEGFERIHRTNLIGMGVLPLEFRPGTNRLTLGLDGTETYDVIGAREPRADVT
                     LVIHRRSGERLEVPMTCRLDTAEEVSIYEAGGVLQRFAQDFLATNAAAAPAAG"
     gene            1193..2377
                     /gene="prpF"
                     /locus_tag="VIN75_16485"
     CDS             1193..2377
                     /gene="prpF"
                     /locus_tag="VIN75_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEY8975820.1"
                     /translation="MAFAPQIRIPATYMRGGTSKGVFFRLQDLPEAAQLPGKARDALL
                     LRVIGSPDPYGKQIDGMGAATSSTSKAVILSKSDRLGHDVDYLFGQVSIDQPFVDWSG
                     NCGNLSAAVGPFAISNGLVDAARVPRDGVAVVRIWQANIGKTIVAHVPMTDGAVQETG
                     DFELDGVTFAAAEVQLEFMDPAAEEEGAGGAMFPTGNLVDELEVPGVGRLRATMINAG
                     IPTIFVEAEAIGYKGTELQDAINGDAAALARFEAIRAHGAVRMGLIANVGEAAGRQHT
                     PKVAFVARSAGYTASSGKPVAAGDIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGT
                     LVNLAAGGGERSAVRFGHPSGTLRVGAQAARVDGEWRVTKAVMSRSARVLMEGWVRVP
                     AG"
BASE COUNT          412 a          931 c          938 g          366 t
ORIGIN      
        1 gcaggagatc gtcgagcgcg atctctacgc cacggcggtg ctttcgggca accgcaactt
       61 cgacggccgc atccatcccc atgcgaagca ggctttcctg gcctcgccgc cgctggtcgt
      121 ggcatacgcg atcgcgggca cgatccgctt cgacatcgag cgcgacgtgc tcggcgtggc
      181 cggcgacggc cgcgagatcc gcctggccga cctctggccg gccgacgagg agatcgacgc
      241 catcgtcgcg gcctccgtac ggcccgagat gttccgcaag gtctacgagc cgatgttcgc
      301 gatccacgcg gaccgcggcg agaaggtgag cccgcagtac gactggcggc cgcagtcgac
      361 ctacatccgg cgcccgccct actgggaccg cgagggcgtg ggcgcgctgg cggccaaccc
      421 gcgcacgctg cgcggcatgc ggccgctggc gctgctgccc gacaacatca cgaccgacca
      481 cctgtcgccc tccaacgcga tcctgcccga cagcgccgcc ggcgagtacc tcgcgaggat
      541 gggcctgccg gaggaagact tcaactccta cgccacgcac cgcggcgacc acctgacggc
      601 catgcgcgcc accttcgcca atccgcagct cgtcaacgag atggcggtgg tcgacggcgt
      661 gcagcgcaag ggctcgcttg cgcgcatcga gcccgagggc agggtggtgc gcatgtggga
      721 ggcgatcgag gtctacctgg agcgcaggca gccgctgatc atcgtggcgg gcgccgacta
      781 cggccagggc tcctcgcgcg actgggcggc caagggtgtg cgcctcgcgg gcgtggaggc
      841 catcgcggcc gaaggcttcg agcgcatcca tcgcaccaac ctgatcggca tgggcgtgct
      901 gccgctcgag ttcaggcccg gcaccaaccg cctgacgctg ggcctggacg gcaccgagac
      961 ctacgacgtg atcggcgcgc gcgagccgcg tgccgatgtg acgctggtca tccaccgccg
     1021 cagcggcgaa cggctcgaag tgccgatgac ctgccgcctc gacaccgcgg aggaggtgtc
     1081 gatctacgag gccggcggcg tgctgcagcg cttcgcgcag gacttcctcg ccacgaacgc
     1141 agcggcggcg cccgcggccg gctgaaccga caatcccgac aatcgcacga ccatggcctt
     1201 cgcaccacag atcaggattc ccgccaccta catgcgcggc ggcaccagca agggcgtgtt
     1261 cttccgcctg caggacctgc ccgaagccgc gcagttgccc ggcaaggcgc gcgacgcgct
     1321 gctgctgcgc gtcatcggca gccccgaccc ctacggcaag cagatcgacg gcatgggcgc
     1381 cgccacctcg agcaccagca aggcggtgat tctttcgaag agcgacaggc ttggccatga
     1441 cgtggactac ctcttcggcc aggtgtcgat cgaccagccc ttcgtcgact ggagcggcaa
     1501 ctgcggcaac ctctcggcgg cggtcggccc gttcgcgatt tccaacggcc tggtcgatgc
     1561 ggcccgcgtg ccgcgcgacg gcgtggcggt ggtgcgcatc tggcaggcca acatcggcaa
     1621 gaccatcgtc gcgcacgtgc cgatgaccga tggcgcggtg caggagaccg gcgacttcga
     1681 gctcgacggc gtgaccttcg cggccgccga ggtgcagctc gaattcatgg accccgcggc
     1741 ggaagaggaa ggcgccggcg gcgcgatgtt cccgaccggc aacctcgtcg acgagctcga
     1801 ggtgcccggc gtcggcaggc tgcgcgcgac gatgatcaac gccggcatcc cgacgatctt
     1861 cgtggaggcc gaagccatcg gctacaaggg caccgagctg caggacgcca tcaacggcga
     1921 cgccgccgcg ctcgcacgct tcgaggccat ccgtgcgcac ggcgcggtgc gcatggggct
     1981 catcgcgaac gtcggcgaag cggcggggcg gcagcacacg cccaaggtcg cgttcgtcgc
     2041 gcggtccgcg ggctacaccg cctccagcgg caagccggtc gcggcgggcg acatcgacct
     2101 gctggtgcga gccctttcga tgggcaagct gcatcacgcg atgatgggca cggcggcggt
     2161 ggccatcggc acggcggctg ccattccggg cacgctggtc aacctcgcgg ccggcggcgg
     2221 cgagcgctcg gcggtgcgct tcggccatcc ctcgggcacg ctgcgcgtgg gtgcgcaggc
     2281 ggcgcgggtc gatggcgaat ggcgcgtgac caaggccgtc atgagccgca gcgcccgcgt
     2341 gctgatggaa ggctgggtgc gcgtgcccgc gggctgaagc gcggggcagg gcgactcgct
     2401 tttgccatcg cggcgtggtc agctagcctg acggtcgcat ccgggcgctt cgaacaggcc
     2461 ttcgcggtga ggagacgagt ccatgagcac gcacgacatc cgctccgcca ggcgtcccga
     2521 tccggacccg cccatggtcg aggtggccga ttacgtcctc agtgacggca tcggttccgc
     2581 cgaggcctat gagacggcgc gctacgtgct gctcgattcg ctggccacgg cgatgatggc
     2641 gatgaag
//
LOCUS       DATCRO010000606         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_760072, whole genome shotgun sequence.
ACCESSION   DATCRO010000606 DATCRO010000000
VERSION     DATCRO010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760072"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VIN75_16490"
     CDS             complement(<1..1089)
                     /locus_tag="VIN75_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8975821.1"
                     /translation="MTLRSIYRRAALALALGLASLAAWAGDAAASAPPAEAASAPREL
                     LVMLRAPTAHARIDGSYGGSGYGDAARAQSQARVGARLAAAHGFTVVTLWALPALGMD
                     CVVLALPPGADVQTSIAALQAHPEVEWAQAMNEFHAQGHADPLYALQPAAAQWHLDDL
                     HAVATGRKVHVAIIDSAVDDTHPDLAQAVLARANFVDDRAWAPELHGTAVAGLVAARA
                     DNGMGIVGIAPDAQLYALRACWEVSATRTLCNSLSLAKALSFAIERRAEVINMSLSGP
                     DDLLLGRLIDVALSHRQQVVAAVDAHAIGGGFPAAHPGVIAVADAQSDAPPAGLSGAW
                     AAPARDLPTTVPGGGWRMVSGTSFAAGEV"
     gene            complement(1086..1775)
                     /locus_tag="VIN75_16495"
     CDS             complement(1086..1775)
                     /locus_tag="VIN75_16495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8975822.1"
                     /translation="MSGQILRLEPDPHVAVQRLLPWYLTGRLETAEHDIVETHLAQCP
                     ECRAELETERQWQLLHPGHGAQVDVEEGWARMRARLSGELPVTAPAPLITPLPAAAPR
                     RRGWLPPAWRTAPRLPARAWAAPALLSAGLAAAIVFTLRPVAPAAQYIALSAPAEPGA
                     TAVVRFKPDATEAQIRHGLKDSGARLVDGPTVSDAYVVRLPREHYAAALDKLRKEPGV
                     ALVEALESASP"
     gene            complement(1846..2469)
                     /locus_tag="VIN75_16500"
     CDS             complement(1846..2469)
                     /locus_tag="VIN75_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8975823.1"
                     /translation="MNTAPTPQPSSRPAAAAGGLHDDAEDRRLVACVCAHELAAFESL
                     YRRYHPRLCRFLHRMLRPEHLVEEVLNDTMYVVWNQAERFNGTSKVSTWIFAIAYRKA
                     LKALSRHEDPVPTTAEDDADHPAAEPSERPEARMSLAQARAAIDTALRALSADQRAAV
                     ELTYFHGFSYPEIAQIVDCPTDTVKTRMFHARKRLRTAFGGQLDDWL"
BASE COUNT          315 a          913 c         1007 g          326 t
ORIGIN      
        1 gacctcgccc gccgcgaacg aggtgccgct gaccatgcgc cagccgccgc ccggcacggt
       61 ggtgggcagg tcgcgcgcgg gcgcggccca cgcgccggac aggccggccg gcggcgcgtc
      121 ggactgcgcg tcggccaccg cgatcacgcc cggatgcgcc gccgggaagc cgccgccgat
      181 cgcgtgcgcg tcgaccgccg ccacgacctg ctggcgatgc gacagcgcca cgtcgatcag
      241 ccgccccagc aacaggtcgt cgggtccgga caggctcatg ttgatgacct cggcgcggcg
      301 ctcgatcgcg aagctcagcg ccttggccag gctcagcgag ttgcacagcg tgcgcgtcgc
      361 gctgacttcc cagcacgcgc gcagcgcata cagctgcgcg tcgggcgcga tgccgacgat
      421 gcccatgccg ttgtccgcgc gcgcggccac caggcccgcc acggcggtgc cgtgcagctc
      481 gggcgcccag gcacggtcgt ccacgaagtt ggcgcgtgcc aggacggcct gcgcgaggtc
      541 ggggtgggtg tcgtcgacgg cgctgtcgat gatggccacg tgcaccttgc ggccggtggc
      601 caccgcgtgc aggtcgtcca ggtgccactg cgcggccgcg ggctgcagcg cgtagagcgg
      661 gtcggcgtgg ccctgggcgt ggaactcgtt catcgcctgc gcccattcga cctcgggatg
      721 cgcctgcaac gcggcgatgc tcgtctggac gtccgccccg ggcggcagcg cgagcacgac
      781 gcagtccatg cccagcgcgg gcagcgccca cagcgtgacg accgtgaagc cgtgcgcggc
      841 ggccaggcgc gcgcccacgc gggcctgcga ctgcgcgcgc gcggcatcgc cgtagccgct
      901 gccgccatag ctgccgtcga tgcgcgcatg cgcggtggga gcgcgcagca tcaccagcag
      961 ctcgcgcggg gccgaggcgg cctccgcggg cggcgccgac gcggcggcgt cgccggccca
     1021 ggcggccagc gacgccaggc ccagcgcgag ggccagggcg gcgcggcggt agatcgaacg
     1081 aagggtcatg gcgatgcgct ctccagcgcc tccaccagcg ccacgcccgg ctccttgcgc
     1141 agcttgtcca gggccgcggc gtagtgctcg cgcggcaggc gcacgacgta ggcatcgctc
     1201 acggtgggcc cgtccaccag ccgcgcgccg ctgtccttga ggccgtggcg gatctgcgcc
     1261 tcggtggcgt cgggcttgaa gcggacgacc gccgtggcgc cgggctcggc gggcgccgac
     1321 agggcgatgt actgcgccgc cggcgcgacc ggccgcagcg tgaagacgat ggccgccgcc
     1381 agaccggccg acagcagcgc cggcgccgcc caggcgcgcg cgggcaggcg cggcgccgtg
     1441 cgccaggcgg gcggcagcca gccgcggcgg cgcggggcgg ccgcgggcag gggcgtgatc
     1501 agcggcgccg gcgccgtcac cggcagctcg ccggacaggc gggcgcgcat gcgggcccag
     1561 ccttcctcga cgtccacctg cgcgccatgg cccgggtgca gcagctgcca ctggcgctcg
     1621 gtctcgagct cggcgcggca ctcggggcac tgcgccaggt gggtttcgac gatgtcgtgc
     1681 tccgcggtct cgaggcggcc cgtgaggtac cacggcagca gccgctgcac cgcgacgtgc
     1741 ggatcgggtt cgaggcgaag gatctgaccg ctcatgccag tctccagaaa cgatggggcc
     1801 gcgccccgga cgtgatcatc ggccatttcg gctcggcgtg ggaagtcata gccagtcgtc
     1861 cagttggccg ccgaacgccg tcctcaggcg cttgcgggcg tggaacatgc gcgtcttgac
     1921 cgtgtcggtg gggcagtcga cgatctgcgc gatctcgggg tagctgaagc cgtggaagta
     1981 ggtcagctcg acggcggcgc gctggtcggc cgacagcgcc cgcagcgcgg tgtcgatcgc
     2041 cgcccgcgcc tgggccaggc tcatgcgcgc ctccgggcgc tcggacggct cggccgccgg
     2101 gtggtcggcg tcgtcctcgg ccgtggtggg caccggatcc tcgtggcgcg acagcgcctt
     2161 gagcgccttg cggtaggcga tggcgaagat ccaggtcgac accttgctcg tgccgttgaa
     2221 gcgctcggcc tggttccaga cgacgtacat cgtgtcgttg agcacctcct cgacgaggtg
     2281 ctccggccgc agcatgcggt gcaggaagcg gcacaggcgc gggtggtagc ggcggtacag
     2341 cgattcgaag gcggccagct cgtgcgcgca gacgcaggcc accaggcggc ggtcctcggc
     2401 gtcgtcgtgc aggccgccgg cggccgcggc gggccgggag gatggctggg gtgtgggggc
     2461 ggtgttcatg gctggcgggg gcttcgtcca ccgtgcatgc cgtcgagcgt gggaagggtt
     2521 caatcgtcgc gcacgggccg cggcgatgca cggcccaggc g
//
LOCUS       DATCRO010000607         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_29139, whole genome shotgun sequence.
ACCESSION   DATCRO010000607 DATCRO010000000
VERSION     DATCRO010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29139"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VIN75_16505"
     CDS             complement(<1..380)
                     /locus_tag="VIN75_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975824.1"
                     /translation="MDSLIPLFQHLLHADQYLAGLVASNGAWVYGILFAVIFIETGVV
                     VMPFLPGDSLLFLVGGLAASGAMDISMALGVMFAAAVIGNQTNYTIGRWIGPRVFHWE
                     NSRWFNKEGFDKSHAFFEKYGGAAL"
     gene            419..>2190
                     /gene="mutL"
                     /locus_tag="VIN75_16510"
     CDS             419..>2190
                     /gene="mutL"
                     /locus_tag="VIN75_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY8975825.1"
                     /translation="MSLQTAAPGRRSIRELPDELVSQIAAGEVVERPASVVRELVDNA
                     LDAGSAQVNVRLVAGGVRAILIEDDGCGIEAAELPLALKRHATSKIRSLDDLEMVSTM
                     GFRGEALAAIASVSELALVSRTPDAAHAQRLDARSGELAPAARAQGTTVEVRELFFST
                     PARRKFLKSDSTELAHCVEAVRRHALVRPQVGFAVWHEGKLVEQWRAADEAQRIRDVL
                     GEDFVDNSRPLAAEIGPIAIHGRVGLPAAARTRSDHQYVYVNGRYVRDRLIAHALRAA
                     YEDVLHGQKQPAYVLFIEIDPARVDVNVHPTKIEVRFRDSGAIHQAVKRAVEDVLAPS
                     GATTPSATHAGTGVVGSSFGTPGGFAAAAAAAVERLNGNGGTATPGWSAAGLAPARPW
                     PVAEDSRPMLAGAATASLFARAFGDSSAPAAAAGGVLDSGGTAVLEANEPVVAAPAWT
                     RPQGNDDAVAAIARADAADWPLGRAIAQVQGVYVLAENAHGLVVVDMHAAHERIVYER
                     LKAGAATHDMPTQALLIPATFAATPAEHALVETHAETLRSLGLDVSPMGPQTLAVRSL
                     PAALLQADPVELVRSVLADLAEVDA"
BASE COUNT          319 a          811 c          783 g          277 t
ORIGIN      
        1 agcgcggcgc cgccgtactt ctcgaagaac gcgtgcgact tgtcgaagcc ttccttgttg
       61 aaccagcgcg agttctccca gtggaagacg cgcggcccga tccagcggcc gatcgtgtag
      121 ttggtctggt tgccgatcac cgcggcggcg aacatcacgc ccagcgccat ggagatgtcc
      181 atcgcgccgc tggcggccag cccgcccacc aggaacagca gcgaatcccc cggcaggaac
      241 ggcatcacga cgacgcccgt ctcgatgaag atgacggcga acaggatgcc gtacacccac
      301 gcgccgttgc tggccaccag gcccgccagg tactggtcgg cgtgaagtag atgctgaaac
      361 aggggaatca aggagtccat gggcggcgat tatcgacggg ccttcctaca atacggggat
      421 gagtctccag accgcggcgc ccgggcgccg cagcatccgt gaattacccg acgaactggt
      481 gagccagatc gccgcgggcg aggtcgtcga acggcccgcg tccgtggtgc gcgagctggt
      541 cgacaacgcg ctcgatgccg gctccgcgca ggtcaacgtg cggctcgtgg caggcggcgt
      601 gcgcgccatc ctgatcgagg acgacggctg cggcatcgag gcggccgagc tgccgctcgc
      661 gctcaagcgc cacgcgacca gcaagatccg ctcgctcgac gacctcgaga tggtctcgac
      721 catgggcttc cgcggcgagg cgctggcggc catcgcgtcg gtctccgagc tggcgctggt
      781 cagccgcacg cccgacgccg cgcacgcgca gcggctggac gcgcgctcgg gcgagctcgc
      841 gccggccgcg cgcgcgcagg gcaccaccgt cgaggtgcgc gagctgttct tcagcacgcc
      901 ggcgcgccgc aagttcctca agtccgattc caccgagctc gcgcactgcg tcgaggccgt
      961 gcgccgccat gcgctggtgc ggccgcaggt gggcttcgcg gtgtggcacg agggcaagct
     1021 cgtcgagcaa tggcgcgccg ccgacgaggc gcagcgcatc cgcgacgtcc tcggcgagga
     1081 cttcgtcgac aacagccgcc cgctggccgc cgagatcggc ccgatcgcca tccatggccg
     1141 cgtcgggctg cccgccgcgg cgcgcacgcg cagcgaccac cagtacgtct acgtcaacgg
     1201 ccgctacgtg cgcgaccgcc tgatcgcgca cgcgctgcgc gccgcctacg aggacgtgct
     1261 gcacggccag aagcagccgg cctacgtgct gttcatcgag atcgacccgg cgcgcgtcga
     1321 cgtcaacgtg catccgacca agatcgaggt gcgcttccgc gactcgggcg cgatccacca
     1381 ggcggtcaag cgcgcggtgg aggacgtgct ggcgccgtcg ggcgccacga cgccgagtgc
     1441 gacgcacgcc ggaacgggcg tggtcggcag ttcgttcggc acgccgggcg gcttcgcggc
     1501 cgcggccgcc gcggccgtcg agcgcctcaa cggcaatggc gggacggcga cgcccggctg
     1561 gagcgcggcc ggcctcgcgc cggcgcggcc ctggccggtc gccgaggaca gccgaccgat
     1621 gctggccggc gccgccacgg catccctgtt cgcgcgcgcc ttcggcgatt cgagcgcgcc
     1681 ggcggctgcg gccggcggcg tgctcgactc gggcggcacc gccgtccttg aggcaaacga
     1741 acccgtcgtc gccgcgccgg cctggacgcg cccgcagggc aacgacgacg ccgtcgcggc
     1801 gattgcccgg gccgacgccg ccgactggcc gctcggacgc gccatcgcgc aggtccaggg
     1861 cgtgtacgtg ctggccgaga acgcgcacgg gctggtcgtc gtcgacatgc acgccgcgca
     1921 cgagcgcatc gtctacgaac gcctgaaggc cggcgccgcc acgcacgaca tgcccacgca
     1981 ggcgctgctg atccccgcca ccttcgccgc gacgccggcc gagcatgcgc tcgtcgagac
     2041 gcatgcggag acgctgcgca gcctcggcct ggacgtctcg ccgatgggcc cgcagacgct
     2101 ggccgtgcgc agcctgcccg cggcgctgct gcaggccgac ccggtcgagc tcgtgcgctc
     2161 ggtgctggcc gacctcgccg aggtcgacgc
//
LOCUS       DATCRO010000608         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_951226, whole genome shotgun sequence.
ACCESSION   DATCRO010000608 DATCRO010000000
VERSION     DATCRO010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951226"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VIN75_16515"
     CDS             complement(<1..874)
                     /locus_tag="VIN75_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975826.1"
                     /translation="MKAPPARDDPPTAIRGLGNWHRDKRRGSAIVFVVAVLLLALLCS
                     AIVGVLSSARAYVGGESLYSKGQKDAVRFLGQYLRSGDEADWQRHLAALAVPLGDRAA
                     QLELEKPRPDFEVVRRGLLQGGNDPADLDLIMGFYRLFRHVPLVAAAVDTWAAADEDI
                     VRLQRMGEQIRQAHDAGGDDAWRPRFEAEMYRLDDALNQRELLFSRQLGQAARWVQGL
                     LLLATAGLALALIAGGLMFARQMWKQLAQSQQEVEDGNARWSLAADAGRLGLMEWRVR
                     DDTILLDPHAIAIYE"
     gene            complement(1041..2495)
                     /locus_tag="VIN75_16520"
     CDS             complement(1041..2495)
                     /locus_tag="VIN75_16520"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8975827.1"
                     /translation="MTENTTASYPDTRLLIEGEWRDATGGRKIPVANPATGQTIGHVA
                     HAAVADLDAALAAAQKGFEAWRNIPAVERAATMRKAAGLLRERADAIARLLTQEQGKP
                     LAEAKVEVLAGAGIIEWFADEGMRVYGRIVPSRNLAAQQLVLKEPVGPVAAFTPWNFP
                     INQVVRKLGAALATGCSFLVKAPEETPASPAALLQTFVDAGVPAGTVGLVFGDPGEIS
                     SHLIASPIIRKVTFTGSTPVGKQLAALAGQHMKRVTMELGGHAPVIVAEDADVALAVK
                     AAGAAKFRNAGQVCISPTRFLVHSSLRDEFAAALVKHAQGLKLGDGLAAGTNLGPLAN
                     GRRVTAMDKVMADARAKGAKIATGGERVGSAGNFFAPTVLTDVPLDADVFNNEPFGPV
                     AAIRAFDTLDEAIAEANRLPYGLAGYAFTRSIKTVHQLSQRLELGMLWINQPATPSAE
                     MPFGGVKDSGYGTEGGPEALEPYLVTKAVSVLGV"
     gene            2667..3848
                     /locus_tag="VIN75_16525"
     CDS             2667..3848
                     /locus_tag="VIN75_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975828.1"
                     /translation="MSVSLANDLQKLIEQSCNTTWSQFDWVKAHAALYGLSRRTADKW
                     TTPGEPAFVSMLVDSPVLRRRLAKAIQAALPPESSKELPLVSGVFHASGPKVRFGKSA
                     TQVELADLLLVRHHFRTGETRPDGRALLLRAAASTTPRTGRLTGRAAHGFDLHADWAT
                     PIAFPNGDFGPPPGGDRWILAQGPSPELPSSVYGIVSSQRSCKGQKFPDNCPWAVGVA
                     LPPEPGHPRESRGTLSLAAALDGFVAGRHGRPWSTAADSQDHWSAFVREALERSVPWE
                     HQVQRVGGVDLPRKRAALAYVQSFLAVEVNAKLTSTIDREHSFEAYVDDLIASVDAVG
                     RQAKEWVASSNATEEPPAPRAAPEGPRAGLSVLYIATFGDAPLAAPQADWVPPPTTPV
                     G"
     gene            complement(4120..4196)
                     /locus_tag="VIN75_16530"
     tRNA            complement(4120..4196)
                     /locus_tag="VIN75_16530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4160..4162),aa:Arg,seq:acg)
BASE COUNT          583 a         1513 c         1502 g          602 t
ORIGIN      
        1 gctcgtagat ggcgatggcg tgcggatcca gcaggatcgt gtcgtcgcgc acccgccact
       61 ccatcaggcc caggcggccg gcgtcggcgg cgagcgacca gcgcgcgtta ccgtcctcga
      121 cctcctgctg cgactgcgcg agctgcttcc acatctggcg ggcgaacatg aggccgccgg
      181 cgatcagggc cagcgccagg ccggcggtgg ccagcagcag cagcccctgc acccagcgcg
      241 cggcctgccc cagctggcgc gagaacagca gctcgcgctg gttgagcgcg tcgtcgagcc
      301 ggtacatctc ggcctcgaag cgcggccgcc aggcgtcgtc gccaccggcg tcgtgtgcct
      361 ggcggatctg ctcgcccatg cgctgcagcc gcacgatgtc ctcgtcggcg gcggcccagg
      421 tgtcgaccgc cgcggccacc agcggcacgt ggcggaacag gcggtagaag cccatgatca
      481 ggtcgaggtc ggcgggatcg ttgcccccct gcagcaggcc acgccgcacg acctcgaagt
      541 cgggccgggg cttctcgagt tccagctgcg ccgcgcgatc gcccagcggc acggccagcg
      601 cggcgagatg gcgctgccag tcggcctcgt cgcccgagcg caggtactgg cccaggaagc
      661 gcaccgcatc cttctggccc ttggaataca ggctctcgcc gccgacatac gcccgggccg
      721 acgacagcac gcccacgatg gccgagcaca gcagcgcgag caggagcacg gccaccacga
      781 agacgatggc tgacccgcgc cgcttgtcgc ggtgccagtt gccgaggccg cgtatcgcgg
      841 tcgggggatc gtcgcgcgcg ggcggcgcct tcatgcgcgc atccgctgca tcgggcagga
      901 gggccgcccg tacgccgcgc tgtcagtcct tgccatggac ctgttcctcg ccttggacct
      961 tgcagtttgc agcaagtccg cgacgttgaa caggtcggcc ggcgggtggc cgccgacttt
     1021 catcgtcggc ggccgcgtcc tcagacgccg aggacggaca ccgccttggt caccaggtag
     1081 ggctccagcg cctcggggcc gccttccgtg ccgtagccgg agtccttgac gccgccgaac
     1141 ggcatctcgg ccgacggcgt ggccggctgg ttgatccaca gcatgcccag ctcgaggcgc
     1201 tggctcagct ggtgcacggt cttgatggac ctggtgaacg cgtagccggc caggccgtac
     1261 ggcaggcggt tggcctcggc gatcgcctcg tccagcgtgt cgaacgcgcg gatcgcggcg
     1321 acgggaccga agggctcgtt gttgaacacg tcggcgtcca gcggcacatc ggtcagcacc
     1381 gtgggtgcga agaagttgcc ggcgctgccc acacgctcgc cgccggtggc gatcttcgcg
     1441 cccttggcgc gcgcgtcggc catcaccttg tccatcgcgg tcacgcggcg gccgttggcg
     1501 agcgggccca ggttggtgcc cgcggccagg ccgtcgccca gcttcaggcc ctgcgcatgc
     1561 ttgacgagcg ccgcggcgaa ttcgtcgcgc aggctgctgt gcaccaggaa gcgcgtcggc
     1621 gagatgcaga cctggccggc gttgcggaac ttggcggcgc cggcggcctt gacggccagc
     1681 gcgacgtccg cgtcctcggc gacgatcacc ggcgcgtggc cgcccagttc catcgtcacg
     1741 cgcttcatgt gctggccggc gagcgccgcg agctgcttgc ccaccggggt cgagccggtg
     1801 aacgtgacct tgcggatgat ggggctcgcg atcaggtggc tcgagatctc gcccggatcg
     1861 ccgaacacca ggcccaccgt gccggccggc acgccggcgt cgacgaaggt ctgcagcagc
     1921 gcggcgggcg acgcgggcgt ctcctcgggc gccttcacca ggaacgagca gcccgtggcc
     1981 agcgccgcgc cgagcttgcg cacgacctgg ttgatcggga agttccacgg cgtgaatgcg
     2041 gcgaccgggc cgacgggttc cttgagcacg agctgctgcg cggccaggtt gcgcgagggc
     2101 acgatgcggc cgtagacgcg catgccttcg tcggcgaacc actcgatgat gccggcgccg
     2161 gccagcacct cgaccttcgc ctcggccagc ggcttgccct gctcctgcgt caacaggcgc
     2221 gcgatcgcgt cggcgcgctc gcgcagcagg ccggcggcct tgcgcatcgt cgcggcgcgc
     2281 tcgaccgcgg ggatgttgcg ccaggcctcg aagcccttct gcgcggcggc cagcgcggca
     2341 tcgaggtcgg cgaccgccgc gtgtgcgacg tggccgatgg tctggccggt ggcggggttg
     2401 gcgacgggga tcttcctgcc gccggtggcg tcgcgccatt cgccttcgat cagcaggcgg
     2461 gtgtcggggt aggaagcggt ggtgttctcg gtcattgaca ggggtccgga tggagtgggt
     2521 accgcaaggt tacctcccgg gcaaaggcct tgcggcccgg gacgccaagc ccgggcactc
     2581 ttcatggatc catgcgaatt tgcgtatggt ccggccggaa cgggccgccg gcgcgaatag
     2641 actggcgccc cctgcccgtc catcgcgtgt ccgtgtccct cgccaacgat ctacagaagc
     2701 tcatcgagca gtcctgcaac accacgtggt cgcaattcga ctgggtcaag gcgcatgccg
     2761 cgttgtacgg cctgtcgcgc cgcaccgcgg acaagtggac gacgcccggc gagccggcgt
     2821 tcgtctcgat gctggtggac tccccggtgc tgcggcgccg gctcgccaag gcgatccagg
     2881 ccgcgctgcc gcccgagtcg agcaaggagt tgccgctcgt gtccggcgtg ttccatgcct
     2941 cgggccccaa ggtccgcttc ggcaagagcg cgacgcaggt cgaactggcg gacctgctgc
     3001 tcgtgcgcca ccatttccgc accggcgaga cgcgccccga cggccgcgca ctgctgctgc
     3061 gcgccgcggc ctcgacgacg ccgcgcacag gccgcctcac ggggcgcgcg gcccacggct
     3121 tcgacctgca cgccgactgg gccacgccca tcgcgttccc caacggcgac ttcggaccgc
     3181 cgcccggcgg cgacaggtgg atcctcgcgc agggcccgtc gcccgagctg ccgtccagcg
     3241 tgtacggcat cgtgtcgagc cagcgctcgt gcaagggcca gaagttcccg gacaactgcc
     3301 cgtgggcggt gggcgtggca ttgccgccgg agccggggca cccgcgtgaa tcgcgcggca
     3361 cgctgagcct ggccgccgcg ctcgacggct tcgtcgcggg ccgccacggc cggccctggt
     3421 cgacggccgc ggactcgcag gatcactggt cggcgttcgt gcgcgaggcg ctcgagcgct
     3481 cggtgccgtg ggagcaccag gtgcagcgcg tcggcggcgt ggacctgccc aggaagcgcg
     3541 cggcgctcgc ctacgtgcag tccttcctcg ccgtcgaggt caacgcgaag ctcacgtcca
     3601 ccatcgatcg cgagcacagc ttcgaggcct acgtcgacga cctgatcgcc agcgtcgacg
     3661 ccgtgggccg gcaggccaag gaatgggtcg cctccagcaa cgcgaccgag gagccgccgg
     3721 cgccgcgcgc ggcacctgag ggcccgcgcg caggcctgtc ggtgctctac atcgccacct
     3781 tcggcgacgc gccgctggcc gcgccgcagg ccgactgggt gccgccgccg accacgccgg
     3841 tcggctaagc cgcgcccgac cgcgggcgtg tcggtgtcgc gcactggcga tgcgtcgaca
     3901 accgccagcc tgtcatcgct gcgcgcagcg aagcggagtc gcaggatcca ccgcggcgca
     3961 ctgcggagtc tcctcgccgg accgccgtgg atcccgcgac tgcgcgcggg atgacatggg
     4021 gccggcgtgg cgcgcgtcca cgcggaaaca gaccggcagc gcacaaagaa aaacgggcca
     4081 cctcttgcga gatgacccgt tcgtctagaa ccgaaattct ggtgcggctg gcaggaatcg
     4141 aacccacgac cccttggttc gtagccaagt actctatcca gctgagctac agccgcgaag
//
LOCUS       DATCRO010000609         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_231500, whole genome shotgun sequence.
ACCESSION   DATCRO010000609 DATCRO010000000
VERSION     DATCRO010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231500"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VIN75_16535"
     CDS             complement(<1..1425)
                     /locus_tag="VIN75_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY8975829.1"
                     /translation="MYFQKIRHPSNFRFASSHAGETLQMGDAAALRVRVRDLGEDVFH
                     VEFRDDARWPLDPRLLRMHDDAFSGPSTHRLGFDAQGTLRLADAQGASALAGVPGACV
                     GVCGKAWLVQFGRSDQMRFYGQGEKLTGMEKSGKRTKFWNLDVWADHPMPVIVDGQAD
                     PQYAAIPYLLIRQGARWIGVLVDHPGAVWMDTGSNWFFTGRDDHDAPPGLWFGADEGV
                     PAFYVIAGDSMGAVTQRLQRLVGKTPLPPLWSLGHHQCRWGYGGTKDLQMLDAGFAEH
                     AFPNDGLWLDIDYMDDYKVFTTSPSQFGDRAAELKALADKGRRVVPILDPGVKVADGY
                     EVAESGLKAGIFCQNPEGRPYVGFVWPGRTWFPDYSLSEGRAWWAGYARQFRDWGFGG
                     AWIDMNDPSTGAAECDDMRFQRGQWDHWTYHNLYATGMAQATWEGFKAAVPDERPFIL
                     SRSAAAGSSRWTALWTGDNWSNWHH"
     gene            complement(1688..2665)
                     /locus_tag="VIN75_16540"
     CDS             complement(1688..2665)
                     /locus_tag="VIN75_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975830.1"
                     /translation="MSFASRNRIVRIAIAATLAAASAAPALAQTAAPEAASVSAEALM
                     QENWRETIARTDVPHEGCFQAEFPSAVWVETSCSVAPDKVYLPHGHRGPQTVGNGTDY
                     AASVSNLISSSVGTFPTVTGVTSERDGGSNVYSIQLNSNFMSTAACNGHSGCLAWEQF
                     VYSSYEQQAFMQYWLIDYGNSCPSGWNSYSGSCYKNSAAVNVPKQAISVLSQLKMSAT
                     AVAGGNDTLVFTTPTKAYSTTGKDSVTYLATAWQQSEFNIVGDGGGSNATFNSGSSIT
                     VKVAVSHGSTAAPTCASNAGTTGETNNLKLGSCSAVGGATPYIQFKESN"
     gene            2989..3498
                     /locus_tag="VIN75_16545"
     CDS             2989..3498
                     /locus_tag="VIN75_16545"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEY8975831.1"
                     /translation="MGTLIKIAVVIAVAWGGWMVWHNKHKAVAHTFEVHGGEAYDAAS
                     NLTWKRCAVGQNFTDGSCVGGDPGFKWDAAQRTGDSTWRVPTEKELGTLIDPAKVDKG
                     DSPAVDTVVFPGIKPSTPPFWTSSHSNGISAWYVRFNKGSDGIGSEPGKYRQSELNDY
                     GVILVHDGR"
     gene            3608..>5067
                     /locus_tag="VIN75_16550"
     CDS             3608..>5067
                     /locus_tag="VIN75_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975832.1"
                     /translation="MTPHRLAPLALLIGLSATASAAGPGAPPERLLEIRAAHAPLAMS
                     GDGAWRLHIDAHDVLHRVSLADPTDETTVQLPRGVQVLSASGDGRRVAVSTRRECVGL
                     ADFGVPPGALPTLVWRPWITSGDGQPLGPADGAWTAAMPADCGGGPQAAPVAISSDGR
                     LVATLDAVIDAATGRVVASLPDTRPVLRLQFVDHDARLLVAAATLAAAGSSASSSLSF
                     SVWDLASKALVNDIEIAGAPLAARAALQVDFSPQTGALFAVDERRRALAQQAAPAGAT
                     PVPLDLVQYSPGACGAAPRVRAQLADDLGAAMVVDPYGRWIASARPLDAAHDGVELAM
                     GARSVLVVRDLASGRQLQRVTSRYALDGLASTPAGDHVFALASQPVEPETGETIAGWP
                     PVPDDERVIDVALPAAALEATRDTARAWTTGVCREPGETPDARALAHADRVLKPLWTR
                     DLGTEPTRLATSAPCAGASRDASVFATRDGGLWLDQG"
BASE COUNT          687 a         1805 c         1835 g          740 t
ORIGIN      
        1 gtggtgccag ttgctccagt tgtcgcccgt ccacagcgcc gtccaccggc tcgatccggc
       61 ggccgcgctg cgcgagagga tgaacgggcg ctcgtcgggc accgcggcct tgaagccttc
      121 ccaggtggcc tgcgccatgc cggtcgcgta caggttgtgg tacgtccagt gatcccactg
      181 gccgcgctgg aagcgcatgt catcgcactc ggccgcgccg gtgctggggt cgttcatgtc
      241 gatccacgcg ccgccgaagc cccagtcgcg gaactggcgc gcgtagcccg cccaccaggc
      301 gcggccctcg gacagcgaat agtcggggaa ccaggtgcgg cccggccaga cgaagccgac
      361 gtagggccgg ccttcggggt tctggcagaa gatgcccgcc ttcaggccgc tctcggccac
      421 ctcgtagccg tccgcgacct tcacgcccgg gtcgaggatg ggcacgacgc gccggccctt
      481 gtcggcgagc gccttcagtt cggccgcgcg atcgccgaac tgcgaggggc tggtcgtgaa
      541 gaccttgtag tcgtccatgt agtcgatgtc gagccacagg ccgtcgttgg ggaacgcgtg
      601 ctcggcaaag cccgcgtcca gcatctgcag gtccttcgtg ccaccgtagc cccagcggca
      661 ctgatggtgg cccagcgacc acagcggcgg cagcggcgtc ttgcccacca ggcgctgcag
      721 gcgctgcgtg accgcgccca tgctgtcgcc ggcgatcacg tagaacgcgg gcacgccctc
      781 gtccgcgccg aaccacaggc cgggcggcgc gtcgtggtcg tccctgccgg tgaagaacca
      841 gttcgatccg gtgtccatcc agacggcgcc cggatggtcg acgagcacgc cgatccagcg
      901 cgcgccctgg cggatcagaa ggtacgggat ggcggcgtac tgcgggtcgg cctggccgtc
      961 gacgatgacc ggcatcgggt ggtcggccca gacgtcgagg ttccagaact tggtgcgctt
     1021 gccggacttc tccatgccgg tgagcttctc gccctggccg tagaagcgca tctggtcgct
     1081 gcgcccgaac tgcaccagcc aggccttgcc gcacacgccc acgcacgcgc ccggcacgcc
     1141 cgcgagcgca ctcgcgccct gcgcgtcggc aaggcgcagc gtgccctgcg cgtcgaagcc
     1201 gaggcgatgc gtcgacgggc cggagaacgc gtcgtcgtgc atgcgcagca ggcgcggatc
     1261 gagcggccag cgcgcgtcgt cgcggaactc gacgtggaac acgtcctcgc cgaggtcgcg
     1321 cacccgcacg cgcaacgcgg cggcgtcgcc catctgcagc gtctcgcccg catggctgct
     1381 ggcgaagcgg aagttggaag ggtggcggat cttctggaaa tacacgcggg cgtcctgagg
     1441 cgagaggcaa caggcagtct agcgccggtg gttacggcgc gggtacgccg gccgggaggc
     1501 gcttgtcacc gcaggatgcg caggcgcagg cgacgacgcc gtccagcgca tgcgggacgg
     1561 cgtcgaggcc gcgcggcgcc ttggcggcac cggactgtcc cggtcgctga tggtgtcggg
     1621 cccccgcctc gggagggggc gaggcggggt ccgggtcgac caccgcgcag gtggccgtca
     1681 gatcaggtca gttcgattcc ttgaactgga tgtacggcgt ggcgccgccg acggcgctgc
     1741 agctgcccag cttcaggttg ttggtctcgc cggtggtgcc ggcgttggac gcgcaggtgg
     1801 gcgcggccgt cgagccgtgc gacacggcca ccttcaccgt gatgctggag cccgagttga
     1861 acgtggcgtt ggagccgccg ccgtcgccga cgatgttgaa ctccgactgc tgccaggccg
     1921 tggcaaggta ggtgacgctg tccttgccgg tggtgctgta ggccttggtg ggcgtggtga
     1981 agaccagcgt gtcgttgccg ccagcgaccg cggtggccga catcttcagc tgcgacagca
     2041 cggagatcgc ctgcttgggc acgttcaccg cggcgctgtt cttgtagcag ctgccgctgt
     2101 aggagttcca gccgctcggg cagctgttgc catagtcgat cagccagtac tgcatgaagg
     2161 cctgctgctc gtacgacgag tacacgaact gctcccatgc gaggcagccc gagtggccgt
     2221 tgcaggcggc cgtcgacatg aagttcgagt tgagctggat ggagtagacg ttgctgccgc
     2281 cgtcacgctc gctggtcacg cccgtcaccg tggggaacgt gcccacggac gacgagatga
     2341 ggttgctgac gctggccgcg tagtcggtgc cgttgcccac cgtctgcggg ccgcgatggc
     2401 cgtgcggcag gtagaccttg tccggcgcga cactgcagct ggtctcgacc cagaccgcgc
     2461 tggggaactc cgcctggaag cagccctcgt ggggcacgtc ggtgcgcgcg atggtctcgc
     2521 gccagttctc ctgcatcagc gcctcggcgc tcacgctggc ggcctcggga gcggccgtct
     2581 gtgcgagggc cggcgccgcc gaggcagccg ccagggttgc ggcgatggcg atgcggacga
     2641 tgcgattgcg ggatgcgaag ctcatcacgg gatctccggt gggttcggtg gagcgccagt
     2701 gttggagtcc ccctgcggag cgtcaatggg ggaagcgacg gcgatgcatc cctttttccg
     2761 ggtacatccc tgggtatgtc ccgcgtgcgt tcgcgcgagc ttgacgaatc ctgaacgctg
     2821 cattcggcag ccgaaccgcc cgtcgcccgc ataccggatg cggcatcttt cacgcgcgca
     2881 gcgtccggac gcagcggccg gacggcgcat gcgccccgcg ccaagataga gttcgcgcca
     2941 gcgcggccgg ccaccggccc gggattcaac gatacggagg gggaaatcat gggaacgctg
     3001 atcaagatcg cagtcgtcat cgcagtggcc tggggcggct ggatggtctg gcacaacaag
     3061 cacaaggccg tggcgcacac gttcgaggtg catggcggcg aggcctacga cgcagccagc
     3121 aacctcacct ggaagcgctg cgccgtcggc cagaatttca ccgacggcag ctgcgtgggc
     3181 ggcgacccgg gcttcaagtg ggacgccgcg caacgcaccg gcgactccac gtggcgcgtg
     3241 ccgaccgaga aggaactcgg gaccctgatc gatccggcca aggtcgacaa gggcgattcg
     3301 cccgcggtcg acaccgtcgt cttccccggc atcaagccca gcaccccgcc gttctggacc
     3361 agctcgcact ccaacggcat cagcgcctgg tacgtgcgct tcaacaaggg ctccgacggc
     3421 atcggctccg agcccggcaa gtaccgccag tccgagctga acgactacgg cgtgatcctg
     3481 gtgcacgacg gccgctgagc cgccggcctc gcgccgcgaa gtctccgcgg cgtgtgagcg
     3541 ttccttccgc gcgggtcgcg tcgcgcgttg ctatcgtcgg cggcatcgcc accaccgacg
     3601 aacgcacgtg accccgcatc gcctcgcccc gctcgcactg ctcatcggcc tgtccgcgac
     3661 cgcctcggcc gcggggcccg gcgcgccgcc ggagcggctg ctcgagatcc gggccgcgca
     3721 cgccccgctc gccatgtccg gcgacggcgc atggcgcctg catatcgatg cgcacgacgt
     3781 gctgcatcgc gtcagcctgg ccgaccccac ggacgagacg acggtgcagc tgccgcgcgg
     3841 cgtgcaggtg ctgtcggcgt cgggcgacgg ccggcgggtg gcggtctcga cgcggcgcga
     3901 gtgcgtcggc ctggccgact tcggcgtccc gcctggcgcc ttgcccacgc tggtctggcg
     3961 gccctggatc accagcggcg acggccagcc gctggggccg gccgacggcg cctggaccgc
     4021 ggccatgccg gccgattgcg gcggcgggcc gcaggccgcg ccggtggcga tctcgtcgga
     4081 cggccggctc gtcgccaccc tggacgccgt catcgatgcc gccaccggcc gcgtcgtcgc
     4141 ctcgctgccg gacacccgcc ccgtgctgcg cctgcagttc gtggaccacg atgcccggct
     4201 gctggtcgcc gcggccacgc tggccgcggc cggctcgtcc gcttccagca gcctgagctt
     4261 ctcggtgtgg gacctcgcca gcaaggcgct cgtcaacgac atcgagatcg ccggcgcgcc
     4321 gctggccgcg cgcgccgcgc tgcaggtcga cttctcgccg cagaccggcg cgctgttcgc
     4381 cgtcgacgaa cgccgccgcg cgctcgcgca gcaggccgcg cccgccggcg cgacgcccgt
     4441 gccgctggac ctggtgcagt actcgccggg cgcgtgcggc gccgcgccgc gcgtgcgcgc
     4501 ccagctggcc gacgacctcg gcgcggccat ggtcgtcgat ccgtacggcc gctggatcgc
     4561 ctccgcgcga ccgctggatg cggcccacga cggcgtcgag ctggccatgg gcgcgcggtc
     4621 ggtgctggtg gtgcgggacc tggccagcgg ccgccagctg cagcgcgtga cgtccaggta
     4681 cgcgctcgac ggcctcgcgt cgacgccggc cggcgaccac gtgttcgccc tggcgtcgca
     4741 gcccgtcgaa cccgagacgg gcgagacgat cgccggctgg ccgccggtac ccgacgacga
     4801 acgcgtgatc gacgtcgcgc tgcccgccgc cgcgctcgag gcgacgcgcg acaccgcccg
     4861 cgcctggacg acgggcgtct gccgcgagcc gggcgagacc ccggacgccc gcgcgctggc
     4921 gcatgcggat cgggtgctca agccgctgtg gacgcgcgat ctcggcaccg agccgacacg
     4981 gctcgcgaca tcggcgccgt gcgccggcgc atcgcgcgac gcgtcggtgt tcgccacccg
     5041 cgacggcggc ctgtggctcg accaggg
//
LOCUS       DATCRO010000610         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_423605, whole genome shotgun sequence.
ACCESSION   DATCRO010000610 DATCRO010000000
VERSION     DATCRO010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423605"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="VIN75_16555"
     CDS             complement(<1..2195)
                     /locus_tag="VIN75_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY8975833.1"
                     /translation="MTDGRPDPDDLLAQAQEAERAARRGRLRIFFGASAGVGKTCAML
                     AQAQAQRAQGVDVVIGIVETHGRAETAALVGAVPVIPRRDVDHHGRTLPEFDLDAVLA
                     RAPDLALVDELAHSNVAGSRHPKRWQDVEELLAAGIDVWTTMNVQHLESLNDVVNAVA
                     GVRVWETVPDHVFDGADDVVLVDLPPDELLQRLKEGKVYIAPQAERAAANFFRKGNLL
                     ALRELALRRTADRVDTDMLSYRRGLRQVAVWPAREALLACVGPGAQGEKLVRAASRLA
                     SQLGAPWHAVYVETPALQRLPRARREAILKTLALAQDLGARTATLASDAPAPAPALAR
                     YAREHNLMRVVLGRRGGARGGWMRRDTAARLASLAPDLELVQVALPERDEPRGLAELA
                     SRITGDGGAPPTDWRAYGKSVLVCGAVIAATAPLLHVLELTNIVMLLLLAVVYSALRH
                     GRGPAVLAAFVSVAAFDFFDVAPRYDFAVSDVQYLVTFVVMLIVALVIGQLTAGLRYQ
                     ARVATLREDRVRALYELSRDLAAALMPEQIVEIGTRVLQAEFEARAVFLLLDGDDRLV
                     VPDEAGVDASLAGWALAHGEAAGQGTGTLPASAMLYVPLKAPMRVRGVVALALRNPQR
                     LAVPEQRRLLDTFASLLAIALERIHYVDVAQTTTVQMESERLRNALLGAISHDLRTPL
                     AALAGMADSLRLTQPLLAPAHAEIAARLREAALRLNALVNNLLDMARLES"
     gene            complement(2286..2500)
                     /locus_tag="VIN75_16560"
                     /pseudo
     CDS             complement(2286..2500)
                     /locus_tag="VIN75_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793006.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
     gene            complement(2497..2730)
                     /locus_tag="VIN75_16565"
                     /pseudo
     CDS             complement(2497..2730)
                     /locus_tag="VIN75_16565"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2847..2972
                     /locus_tag="VIN75_16570"
     CDS             2847..2972
                     /locus_tag="VIN75_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975834.1"
                     /translation="MGALPQVGFKFGRWLDLAFFQPLLETPGAPVDGRRSRWGSP"
     gene            3231..4157
                     /gene="rpoH"
                     /locus_tag="VIN75_16575"
     CDS             3231..4157
                     /gene="rpoH"
                     /locus_tag="VIN75_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY8975835.1"
                     /translation="MSTTQTNALSVRDPWSLVPPLGNLDAYISSINRLPMLSQEEEHR
                     LATQLQQSGDLKAASALVMSHLRLVVSVSRNYLGYGLPHGDLIQEGNIGLMKAVKRFD
                     PNQGVRLVSYALHWIKAEIHEYILKNWRMVKVATTKSQRKLFFNLRSLKAKLKEEASD
                     ADVHRNTLTRDQVERVAADLNVKPEDVLEMETRMSGGDVAMESQNDDGEEAYAPIAYL
                     ADDRSEPTRVIEAAAHDRLAGAGIVEALDTLDPRSRRIVEERYLKVNDDSSGGMTLHE
                     LAAEYGVSAERIRQIEVAALKKMRKTLEPQRV"
BASE COUNT          668 a         1549 c         1438 g          560 t
ORIGIN      
        1 gactccagcc gcgccatgtc cagcaggttg ttgaccagcg cgttcaggcg cagcgccgcc
       61 tcgcgcaggc gcgccgcgat ctccgcgtgc gccggcgcca gcagcggctg cgtgagccgc
      121 agcgaatcgg ccatgcccgc cagcgcggcc agcggcgtgc gcaggtcgtg cgagatcgca
      181 cccagcagcg cattgcgcag ccgctccgac tccatctgca ccgtcgtcgt ctgggcgacg
      241 tcgacgtagt ggatgcgctc cagcgcgatc gccagcagcg aggcgaaggt gtccagcagc
      301 cggcgctgct cgggcaccgc gagccgctgc gggttgcgca gcgccagggc gacgacgccg
      361 cgcacgcgca tcggggcctt cagcggcacg tacaacatcg cgctggccgg cagcgtgccg
      421 gtgccctgcc ccgcggcctc gccgtgtgcg agcgcccagc ccgcgaggct cgcgtccacg
      481 ccggcttcgt ccggcacgac gaggcggtcg tcgccgtcca gcagcaggaa cacggcgcgc
      541 gcctcgaact cggcctgcag cacgcgggtg ccgatctcga cgatctgctc gggcatcagt
      601 gccgcggcca gatcgcgcga cagctcgtac agcgcgcgca cgcggtcctc gcgcagcgtc
      661 gccacccgcg cctggtagcg caggccggcc gtcagctggc cgatcacgag cgcgacgatc
      721 agcatcacga cgaaggtgac gaggtactgc acgtcgctga ccgcgaagtc gtaccgcggg
      781 gcgacgtcga agaagtcgaa cgccgcgacg ctgacgaagg ccgccagcac cgccggcccg
      841 cggccgtggc gcaacgcgga gtacaccacg gcgagcagga gcagcatcac gatgttggtc
      901 agctccagca cgtgcagcag cggcgcggtg gcagcgatca cggcgccgca cacgagcacg
      961 ctcttgccgt acgcgcgcca gtcggtcggc ggggcgcccc cgtcgccggt gatgcgcgat
     1021 gccagctccg cgaggccgcg cggctcgtcg cgctcgggca aggcgacctg cacgagttcg
     1081 aggtccggcg ccagcgacgc caggcgcgcc gcggtgtcac gccgcatcca gccgccgcgc
     1141 gcgccgccgc gccgcccgag caccacgcgc atcaggttgt gctcgcgcgc gtaacgcgcc
     1201 agcgcgggcg cgggcgcggg cgcgtcgctc gcgagcgtgg cggtgcgcgc gcccaggtcc
     1261 tgcgccagcg ccagcgtctt caggatcgcc tcgcggcgcg cgcgcggcag gcgctgcagc
     1321 gcgggcgtct cgacatagac cgcatgccac ggcgcgccca gctgcgacgc cagccgcgac
     1381 gcggcgcgca ccagcttctc gccctgggca cccgggccca cgcacgccag caacgcctcg
     1441 cgcgccggcc acaccgccac ctgcctgagc ccgcgccggt agctcagcat gtcggtgtcg
     1501 acgcggtcgg cggtgcgtcg cagcgccagc tcgcgcagcg ccagcaggtt gcccttgcgg
     1561 aagaaattgg ccgccgcgcg ctcggcctgc ggcgcgatgt agaccttgcc ttccttcagg
     1621 cgctgcagca gctcgtcggg cggcaggtcg accagcacga cgtcgtcggc gccgtcgaac
     1681 acgtggtcgg gcaccgtctc ccagacgcgc acgccggcca ccgcgttgac gacgtcgttc
     1741 aggctctcga ggtgctgcac gttcatcgtc gtccacacgt cgatgccggc ggccagcagt
     1801 tcctcgacgt cctgccagcg cttgggatgg cgcgagccgg cgacgttgga gtgcgccagc
     1861 tcgtcgacca gcgcgagatc cggcgcgcgc gccagcacgg cgtcgaggtc gaactccggc
     1921 agcgtgcggc cgtggtggtc gacgtcgcgc cgcgggatga ccggcaccgc gccgacgagc
     1981 gccgccgtct ccgcgcggcc atgcgtctcg acgatgccga tcaccacgtc cacgccctgc
     2041 gcccgctgcg cctgggcctg cgccagcatc gcgcaggtct tgcccacgcc ggccgacgcg
     2101 ccgaagaaga tcctcagccg cccgcgccgc gccgcgcgct cggcctcctg cgcctgcgcg
     2161 agcaggtcgt cggggtcggg gcggccgtcg gtcatgtgtc gaactctagc tcgcgggtca
     2221 ccgcactgcc acgctgtcat tccgcgcgcg gtcgcaggat gacggcccgg gcgatgacgg
     2281 ccaggtcagc gcgtccgggc atcgtcgacg tcgaggttga gcagcagcac gttcacccgc
     2341 gcctcgccca ggaagccgaa cagcggctgc tcggtgcgcc ggtcgaccag cgccaggacg
     2401 cgttcgcgcg acagtcccgt cgccccgcca cgcggcccgc ctggtacagc gcggccgcca
     2461 agctgatgtg cggatcgagc ccggtggcgg aggccgtcac atcgcaagca ccggcacgac
     2521 cacgccgaag cggccgaacc acatcgccac cggtgtcgaa tgccgagtgg gcgacacgtt
     2581 gccgaaccgc tgcggattca gcggcagcca ggcttgcacg cgctggacga ggtagctcgc
     2641 gcacaggccg atcgcgttga acaactgcag gcccaggccg tactgcagcc agcccatctg
     2701 gcggttcgcg tcgatgccgc agaggcgatg gaaggcgcgg cggcgcgcaa cgcgatcgcg
     2761 ctgaccgcgg cgtccgacct gttcaggaaa tcccgctgaa cgagcgcacc gtcgtcccct
     2821 gcaccctggc gcaccacggc gcggcgatgg gcgcgctgcc gcaggtcggc ttcaagttcg
     2881 gccgctggct cgacctggcg ttcttccagc cgctgctcga gactcctggc gcgccggtcg
     2941 acggtcgacg gtcgaggtgg ggctcccctt gaaggaacgg tgcggcgact caacatcaag
     3001 cgggtgccgt tccggtaccc ctgccgggcg tgcggcttgc aggtccttgt gagataattt
     3061 ccatagcgag cgatacgtaa agatgtcatt gtgagtgctc gctagcgaga tgatcggcgt
     3121 tctatccacg aatccgagcc agaccttctc gaaaatgagg atcagcactc ttgcctgggg
     3181 agtgctaata tccgggaacc aaaggatcga ttcacgatcc gaccatgcac atgagcacca
     3241 cccaaaccaa cgccctgtcc gtccgagacc cgtggagtct ggttcccccg ctgggcaacc
     3301 tcgacgccta catctcgtcc atcaaccgcc tgccgatgct gtcgcaggag gaagagcatc
     3361 gtctcgcgac gcagctgcag cagtccggcg acctgaaggc tgccagcgcg ctggtgatgt
     3421 cgcacctgcg cttggtcgtg tccgtctcgc gcaactacct gggctacggc ctgccgcacg
     3481 gcgacctgat ccaggaaggc aacatcggac tgatgaaggc cgtcaagcgc ttcgacccga
     3541 accagggcgt gcgcctggtc agctacgcgc tgcactggat caaggccgag atccacgaat
     3601 acatcctgaa gaactggcgc atggtcaagg tcgccacgac caagtcccag cgcaagctgt
     3661 tcttcaacct gcgttcgctc aaggccaagc tcaaggaaga ggcttccgac gccgacgtgc
     3721 atcgcaacac gctcacgcgc gaccaggtcg agcgcgtcgc ggccgacctc aacgtgaagc
     3781 cggaagacgt cctcgagatg gagacgcgca tgtccggcgg cgacgtcgcg atggagtccc
     3841 agaacgatga cggcgaggaa gcctacgcgc cgatcgccta cctggccgac gaccgcagcg
     3901 agcccacccg cgtcatcgag gccgccgcgc atgaccgcct ggccggcgcc ggcatcgtcg
     3961 aggcgctgga cacgctcgac ccgcgcagcc gccgcatcgt cgaggagcgc tacctgaagg
     4021 tcaacgacga cagctccggc ggcatgacgc tgcatgagct ggccgccgag tacggcgtga
     4081 gcgccgagcg catccgccag atcgaggtcg ccgcgctcaa gaagatgcgc aagacgctgg
     4141 aaccgcaacg cgtgtaatgg agccccacgg tcgctatccc ccaaggggag gcttcgcgcg
     4201 cgctccctgc ccccg
//
LOCUS       DATCRO010000611         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_74067, whole genome shotgun sequence.
ACCESSION   DATCRO010000611 DATCRO010000000
VERSION     DATCRO010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74067"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..564
                     /locus_tag="VIN75_16580"
     CDS             340..564
                     /locus_tag="VIN75_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975836.1"
                     /translation="MRTTQLQSLTWGTVTAAIGCAVWLSSSDQAALFCSVGYRATSQA
                     MGFDIPAEPTRAGKAAQAPGSSAARQPEAG"
     gene            809..2128
                     /locus_tag="VIN75_16585"
     CDS             809..2128
                     /locus_tag="VIN75_16585"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY8975837.1"
                     /translation="MKVIVLGAGIIGVATAWHLLEEGHEVTVVDRQPEAALETSFANG
                     AQISVCFCEPWANAGAPFKVAKWMLQADSPLLFRPRLDPHQWRWALSFLTQCTDAAFA
                     RNVEQLVSLGRYSHDALKAVVGKTGIEYDRLERGILHFFSSAADYENGVKAAALMRDH
                     GVDRRILDRDAVLAVEPALKRFGGIHGGTFTPSDESGDARVFTQKLAQLCAARGAKFL
                     WEHDILGLDKSGAKITGVRVRDRGTLRASVLTADQYVAAAGSFTAPLLRPLGIWLNIY
                     PAKGYSATLRLKRPEDASVVSLLDDTRKIAISRLGDHIRIAGTAEMAGYDTSIDSAKS
                     RVRCEALVRRYEELFPGVADTSQPNFWAGLRPSTPNNLPYIGRSKIANLWLNAGHGTL
                     GWTHGAGSGRALAELISGRRPHLDYGFCGEALPARRPLVPMPSRSWA"
     gene            complement(2133..2804)
                     /locus_tag="VIN75_16590"
     CDS             complement(2133..2804)
                     /locus_tag="VIN75_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEY8975838.1"
                     /translation="MTSPAAPTPVPPLAEADLNRLQTLLDKIPEPLEPLDISMLDGYL
                     CGVLLQPTAVPAGAWIRHVFDIDGRPLDDKWKGLHELQGLVQRRYRELATAIAGRQWF
                     DPWVFELDDEADPLECTLPWVAGFSAAMELFPALMDMDSPDTLEPLAVLYAAFDPDDL
                     EDADALLAEIETLEPPADLDEAIEDVVQSVMRLADVSRPLPKARTAPVKRPPPRRGPP
                     PRGRY"
BASE COUNT          461 a         1098 c         1055 g          465 t
ORIGIN      
        1 gctctccagc gcctcgtcga gggcgaaggc ggtgcccatg tcggcgaggt ccggcggacg
       61 ccgcggcgac gtcgcgccgc ggccgaaatt gcgcaggaac gtggggcgtg aggcgttggt
      121 catcggtctc tccaggcttg caccgtgaga ccagtgtgaa ggaacgtttc tcgggcggtg
      181 cggcgaaatg tgtgtcgtat gcccgacagt tccgatgtat cgaaatcacg actcccccga
      241 acggacgatg gcggggtcat ggcatcggac ttaagctgca agcgtcgatg ccgagggtgc
      301 agctgcgcgc ccccgggaac caccttggga gccgccgcca tgcgcaccac gcaacttcaa
      361 tccctcacct ggggcaccgt caccgctgcc atcggctgtg cggtgtggtt gtcgtcgtcc
      421 gaccaggcgg cgctgttctg ctcggtcggc tatcgcgcca ccagccaggc catgggcttc
      481 gacatcccgg ccgaacccac gcgtgccggc aaggcggcgc aggcgccggg ttcgagcgcc
      541 gccaggcagc ccgaggccgg ctgagcaccg gccgtccggt cgcgcgccac cactcgccgc
      601 cgtccgcgga tccgcgaaga acgagccgct gcagcgggca gtctcgccac gcgggatcgc
      661 tccgattcga tcagtagttg atctactgaa cttggaattc gattaatccg gcttcagtta
      721 cttcatcgcc tccccgaaaa tccgggcgca acccgacggt gccgctggtc gtttcccggc
      781 cggccgccga gcggcgaagg aattcgcgat gaaagtgatc gttctgggtg ccggcatcat
      841 cggcgtcgcc accgcctggc acctgctcga agaaggccac gaggtgaccg tcgtcgaccg
      901 ccagccggag gccgcgctgg agaccagctt cgccaacggc gcccagatct cggtgtgctt
      961 ctgcgaaccc tgggccaacg ccggcgcgcc gttcaaggtg gccaagtgga tgctgcaggc
     1021 cgattcgccg ctgctgttcc gcccgcgcct cgatccgcac cagtggcgct gggccctgag
     1081 cttcctcacg cagtgcaccg acgccgcctt cgcgcgcaac gtcgaacagc tggtctcgct
     1141 gggccgctac agccacgacg cgctgaaggc ggtcgtcggc aagaccggca tcgagtatga
     1201 ccgcctcgag cgcggcatcc tgcacttctt ctcgtcggcc gccgactacg agaacggcgt
     1261 caaggccgcg gcgctgatgc gcgaccacgg cgtcgaccgc cgcatcctcg atcgcgacgc
     1321 ggtgctggca gtcgagcccg cgctcaagcg cttcggcggc atccacggcg gcacgttcac
     1381 gcccagcgac gaatccggcg acgcgcgcgt gttcacccag aagctggcgc agctgtgcgc
     1441 ggcgcgcggc gcgaagttcc tgtgggaaca cgacatcctc ggcctggaca agtcgggcgc
     1501 gaagatcacc ggcgtgcggg tgcgcgatcg cggcacgttg cgcgcgagcg tgctgacggc
     1561 cgaccagtac gtcgcggccg ccggctcgtt caccgcgccg ctgctgcgcc cgctgggcat
     1621 ctggctcaac atctacccgg ccaagggcta ctcggccacc ctgcgcctga agcgccccga
     1681 ggacgcgagc gtcgtcagcc tgctggacga cacccgcaag atcgcgatct cgcgcctggg
     1741 cgaccacatc cgcatcgccg gcacggccga aatggccggc tacgacacct ccatcgacag
     1801 cgcgaagtcg cgcgtgcgct gcgaggcgct cgtgcgccgc tacgaggagc tgttccccgg
     1861 cgtggccgac accagccagc ccaatttctg ggccggcctg cgcccgagca cgccgaacaa
     1921 cctgccctac atcggccgca gcaagatcgc caacctgtgg ctcaacgccg gccacggcac
     1981 cctcggctgg acgcacggcg ccggctccgg gcgcgcgctc gccgagctga tcagcggccg
     2041 ccgcccgcac ctggactacg gcttctgcgg cgaggcgctg cccgcgcgcc ggccgctggt
     2101 gccgatgccg tcgcgctcct gggcctgagc cctcagtaac gcccgcgagg aggcggcccg
     2161 cgccgcggcg gcgggcgctt gaccggggca gtccgtgcct tgggcagcgg ccggctgacg
     2221 tcggccaggc gcatcacgct ctgcacgacg tcctcgatcg cctcgtcgag gtcggccggc
     2281 ggctccagcg tctcgatctc ggccagcagc gcgtcggcgt cctcgaggtc gtccgggtcg
     2341 aacgcggcgt acagcacggc cagcggctcg agcgtgtcgg gcgagtccat gtccatcagc
     2401 gccgggaaca gctccatggc ggcggagaag ccggccaccc acggcagcgt gcattcgagc
     2461 gggtcggcct cgtcgtcgag ctcgaacacc caggggtcga accactggcg ccccgcgatc
     2521 gcggtcgcga gctcgcggta gcggcgctgc accaggccct gcagttcgtg cagccccttc
     2581 cacttgtcgt ccagcgggcg gccgtcgatg tcgaacacgt gccggatcca ggcgcccgcg
     2641 ggcacggccg tcggctgcag cagcacgccg cacaggtagc cgtccagcat gctgatgtcc
     2701 agaggctcga gcggctcggg aatcttgtcc aggagcgtct ggagccggtt caggtcggct
     2761 tcggcgagcg gcgggacggg cgtgggggcg gcgggcgagg tcatggcgca ttgtcgcgcc
     2821 atggggacgc catggcatgc gccccgaggc gaaccattcc ggtgcgtcgg acacaccccc
     2881 gcggtgttgc cgcgatgagg cactccccct gaaataggga tgcttttcga caaatcggct
     2941 cgtacattgg attccgttgt gctgatcgag ccaggagctc tgggagggga cgggatcggt
     3001 cggcacgcac agtcccaacg acgtccttct cgcgaaggac ttgaagccca ccctcgcggt
     3061 gggttttttt tcgcccgcg
//
LOCUS       DATCRO010000612         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_287694, whole genome shotgun sequence.
ACCESSION   DATCRO010000612 DATCRO010000000
VERSION     DATCRO010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287694"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIN75_16595"
     CDS             <1..390
                     /locus_tag="VIN75_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8975839.1"
                     /translation="LNNVMLKREAAQPEEGRALAMFLGGAVVAGATAVVSSTSGAAGV
                     QWPVATTAWMLPAVGLALAFMASNLCLQYGAARLSAAATAVVMPCEVLFAALTSVWWG
                     GATLHASVLVGGALILAATLASVLEKR"
     gene            475..966
                     /locus_tag="VIN75_16600"
     CDS             475..966
                     /locus_tag="VIN75_16600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8975840.1"
                     /translation="MAAKTLYDFSANSIQGQPADFGSQRGKVFLIVNTASKCGFTPQF
                     AGLEQLASDYKDRGLVVVGFPCNQFGHQDPGANSEIESFCQLNYGVTFPMMEKVDVNG
                     AKAHPLWKWLKSEKPGVLGTEAIKWNFTKFLVGKDGHVVKRFAPNDEPDKLRADIEAA
                     LAA"
     gene            complement(1132..1815)
                     /locus_tag="VIN75_16605"
     CDS             complement(1132..1815)
                     /locus_tag="VIN75_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8975841.1"
                     /translation="MASELRALFDRNFPEARWTESLWAAAEPRLRVRRLAAGATLFAQ
                     GSVSPALFGVLQGAVEVRFSGAQGQLSVVSHVEAGRTFGLASFVTGLPSAYEALALEP
                     TRLVMVGRAAYDWLMDEVPGFGRALMREFARHFDGNLKLLEAARHRSAEDRLCIALQQ
                     LHGEGRATAGGAGRWTIRLTQSELAMRANVSRQTANEWLAQWAARGWIARRYGALEIM
                     RWPVPDEGA"
     gene            1890..3050
                     /locus_tag="VIN75_16610"
     CDS             1890..3050
                     /locus_tag="VIN75_16610"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEY8975842.1"
                     /translation="MNPMRPDHDPAVIARLQALFATFSGAPLRDDPLCQALCAAAARN
                     ADWAALLAEAPENQRWPMLWLAAIQDRVMALTEAGERPALADYYASVGPARAPDAALE
                     GHLGEFIERHRDVLAHAIATRSTQTNEVGRCVVLWPLLQRLAQATGRRRIALLDVGCS
                     AGLLLGVDRWRYRYIDDASGATVAASPPDRDPRAPEIACRLLAGSCAGLVTGDAAPEI
                     VSRAGVDLNPIAVDDPAAVRWLRACLWPHDGERRARLDAAVAVARTQHWPLRKTSDVV
                     AAVGAWLDELPVDVMPVVVNVWVLSYFDKPLLRRYSDSLLEHVATRGAAWISGDDPAL
                     SRTWWPGRPEGAADQRPNATAWTVARPDGRGGVAWEQPATSHAHGRWMLCEG"
     gene            3108..>3589
                     /locus_tag="VIN75_16615"
     CDS             3108..>3589
                     /locus_tag="VIN75_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8975843.1"
                     /translation="MFEHIEPFAGDPILSLNEDFQKDPRPHKINLSIGIYFDDEGRIP
                     VLDCVRKAEGLLLEKGGAKPYLPIEGNADFRRGVQTLLFGAGSKAVAEGRVVTLQTVG
                     SSSALKVGADFIKRWFPDSGVWVSDPTWDNHRSMFEGAGVKVSTYPYYDAATGGLKFD
                     A"
BASE COUNT          508 a         1320 c         1260 g          501 t
ORIGIN      
        1 ctgaacaacg tgatgctcaa gcgcgaggcg gcgcagcccg aggaaggacg cgcgctggcg
       61 atgttcctgg gcggcgcggt ggtggcgggc gcgacggccg tggtctcgtc gacctcgggg
      121 gcggcgggcg tgcagtggcc cgtggccacc accgcctgga tgctgccggc ggtcggcctc
      181 gcgctcgcgt tcatggcgtc gaacctgtgc ctgcagtacg gcgcggcgcg gctgtcggcc
      241 gcggccaccg cggtggtgat gccctgcgag gtgctgttcg ccgcgctgac ttccgtctgg
      301 tggggcggcg ccacgctgca cgcgagcgtg ctggtgggcg gcgcgctgat cctggccgcc
      361 acgctggcca gcgtgctcga gaagcgctga tccccctgtt gtttcgcgat cacgcgcgcg
      421 ccgcggcggc gcccgccgat ctaccatccc gatcctcacc ttccaggaga gcccatggcc
      481 gccaagacgc tttacgactt ttccgcgaac tccatccagg gccagccggc cgacttcggc
      541 tcgcagcgcg gcaaggtctt cctgatcgtc aacaccgcca gcaagtgcgg cttcaccccg
      601 cagttcgcgg gcctggaaca gctggcgtcc gactacaagg accgcgggct ggtggtggtc
      661 ggcttcccgt gcaaccagtt cggacaccag gatccgggcg cgaactccga gatcgagagc
      721 ttctgccagc tcaactacgg cgtcacgttc ccgatgatgg agaaggtgga cgtcaacggc
      781 gccaaggcgc atccgctgtg gaagtggctg aagtccgaga agcccggcgt gctgggcacc
      841 gaggcgatca agtggaactt caccaagttc ctggtcggca aggacggcca cgtggtcaag
      901 cgcttcgcgc cgaacgacga gccggacaag ctgcgcgccg acatcgaggc tgcgctcgcc
      961 gcgtgaagcg cccgccggcg tcgtcccgac gcgtggatcc tgcgacttcg cgcaggatga
     1021 caggccaagg gagcggctcg cgaagtgcgt tgcccgggtc gccgcgtccg gtcgcgctgc
     1081 atcctgcgcg gagcgaagcg cagtcgcagg atccacgacc gctgccacgc ttcaagcacc
     1141 ttcatccggc acgggccagc gcatgatctc gagcgccccg tagcgccgcg cgatccagcc
     1201 gcgcgccgcc cattgcgcca gccattcgtt ggcggtctgg cgcgacacgt tggcgcgcat
     1261 ggccagctcc gactgcgtga gccggatcgt ccagcggccg gcgccgccgg ccgtggcccg
     1321 gccctcgccg tgcagctgct gcagggcgat gcacagccgg tcttccgcgc tgcggtggcg
     1381 cgcggcctcc agcagtttca ggttgccgtc gaagtggcgc gcgaactccc gcatcagcgc
     1441 gcgcccgaag cccggcacct cgtccatcag ccagtcgtag gcggcgcggc cgaccatcac
     1501 caggcgcgtg ggctccagcg ccagcgcctc gtaggccgac ggcaggcccg tgacgaacga
     1561 ggccaggccg aaggtgcgcc ccgcctcgac gtggctcacg accgacagct gcccctgcgc
     1621 gccggagaac cgcacctcga cggcgccttg cagcacgccg aacagcgccg gcgagacgct
     1681 gccctgcgcg aacagcgtcg cgcccgccgc gaggcggcga actcgcaggc gcggctcggc
     1741 cgcggcccac agggactcgg tccaccgcgc ttcggggaaa ttccgatcga acagggcgcg
     1801 caattcgctg gccatgggcc gattgtcggc tgcttgacaa tcctgcgcgg cccgggggac
     1861 tacgctggcg ccatcgccac tggacaagaa tgaatcccat gcgtcccgac cacgaccccg
     1921 ccgtcatcgc caggctgcag gcactgttcg ccacgttctc gggtgccccc ctgcgcgacg
     1981 acccgctgtg ccaggcgttg tgcgccgccg ccgcgcgcaa cgccgactgg gccgcgctgc
     2041 tggccgaggc gccggagaac cagcgctggc cgatgctgtg gctggccgcc atccaggatc
     2101 gcgtgatggc gttgaccgag gccggcgagc ggcccgcgct ggccgactac tacgccagcg
     2161 tcggccccgc gcgcgcgccc gacgccgcgc tcgaaggaca cctgggcgag ttcatcgagc
     2221 gccatcgcga cgtcctggcg cacgccatcg ccacccgcag cacgcagacc aacgaggtgg
     2281 gccgctgcgt cgtgctgtgg ccgctgctgc agcgcctcgc gcaggccacg gggcggcggc
     2341 gcatcgcgct gctggacgtc ggctgcagcg ccggcctgct cctgggcgtc gatcgctggc
     2401 gctatcgcta catcgacgac gccagcggcg cgacggtggc cgccagcccg cccgatcgcg
     2461 acccgcgcgc gcccgagatc gcgtgccgcc tgctcgcggg ctcgtgcgcg ggcctcgtca
     2521 ccggcgacgc cgcgccggag atcgtctccc gggccggcgt cgacctgaac ccgatcgccg
     2581 tcgacgaccc cgccgcggtg cgttggctgc gcgcatgcct ctggccgcac gacggcgagc
     2641 gccgcgcgcg cctggacgcc gccgtcgccg tggcccgcac gcagcactgg ccgctgcgca
     2701 agacgagcga cgtcgtcgcc gccgtcggcg catggctgga cgagctgccc gtcgacgtca
     2761 tgccggtggt cgtcaatgtc tgggtgctgt cgtacttcga caagccgctg ctgcgccgct
     2821 actcggacag cctgctggag cacgtggcca cgcgcggcgc cgcgtggatc agcggcgacg
     2881 acccggcgct gtcgcgcacc tggtggcccg gcaggcccga gggcgcggcc gaccagcgcc
     2941 ccaacgcgac cgcctggacg gtggcgcgcc ccgacggccg cggcggcgtc gcgtgggagc
     3001 agccggccac gtcgcatgcc catggacgtt ggatgctttg cgaagggtga gggcgggacg
     3061 gctggccgat aatcgaccgg tcgtccctcc aaccgagcca aagccccatg ttcgagcaca
     3121 ttgaaccgtt cgccggtgac ccgatcctgt cgttgaacga ggatttccag aaggatccgc
     3181 ggccccacaa gatcaacctg tcgatcggca tctacttcga cgacgaaggc cgcatcccgg
     3241 tgctcgactg cgtgcgcaag gccgagggcc tgctgctcga gaagggcggc gccaagccgt
     3301 acctgcccat cgagggcaac gccgatttcc gccgcggcgt gcagacgctg ctgttcggcg
     3361 ccggctccaa ggccgtcgcc gagggccgcg tcgtcacgtt gcagaccgtg ggctcgagca
     3421 gcgcgctcaa ggtcggcgcc gacttcatca agcgctggtt ccccgactcc ggcgtgtggg
     3481 tcagcgaccc gacctgggac aaccaccgtt cgatgttcga gggcgcgggc gtcaaggtgt
     3541 ccacctaccc gtactacgac gcggccaccg gcggcctgaa gttcgacgc
//
LOCUS       DATCRO010000613         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_277079, whole genome shotgun sequence.
ACCESSION   DATCRO010000613 DATCRO010000000
VERSION     DATCRO010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277079"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VIN75_16620"
     CDS             complement(<1..861)
                     /locus_tag="VIN75_16620"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8975844.1"
                     /translation="MSPAENTASGTPPMKLSGLEPVTLDAGHLFVNIGERTNVTGSKA
                     FARLILAGEFEQALAVARQQVENGAQIIDVNMDEAMLDSQAAMVRFLNLVASEPEIAR
                     VPVMVDSSKWSVIEAGLKCLQGKGIVNSISMKEGEDEFRRQARLVRRYGAAAVVMAFD
                     EKGQADTFERKIEICERAYRVLVDEVGFPPEDIIFDPNIFAIATGIEEHDNYAVDFIE
                     ATRWIKRNLPGAKVSGGVSNVSFSFRGNEPVREAIHTVFLYHAIRAGMDMGIVNAGMV
                     GVYDDLDPVLR"
     gene            953..2158
                     /locus_tag="VIN75_16625"
     CDS             953..2158
                     /locus_tag="VIN75_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975845.1"
                     /translation="MKVVPLPSEGLRIGRPLPFTVRDGFGAVLLARGSTIQTEKQLQL
                     LRSREIFVDMSDMESVQRMINGQLDQMFRQDVALGRIAAARPDFEGIPLPGQAARPAE
                     RPLDWPNLQMRLRLLLSDPRAADWVARLRTLRDDVLALVERGPDRALMRLMYDASHDF
                     QDYSANHSLFVCVLASMACTQLPGWQADWADALTLASLSMNVSFTQMQDELARQSGPM
                     SDAQRDLMHEHAARAADLLAEVGIDDPIWLHAVRHHHSAPAGPMAGRSPQETVARLIR
                     RADRYAARLSPRKSRPASSATAAAQAALLDEGGHTDEAGQALIRTLGLYPPGVWVKLQ
                     CGEIAMVVRRTPIAKSPVVVSLVGRSGMPLAVPATRNTKLPDYAVTGAVPPGQVNVRP
                     NLDVLEKLG"
     gene            complement(2166..3128)
                     /locus_tag="VIN75_16630"
     CDS             complement(2166..3128)
                     /locus_tag="VIN75_16630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8975846.1"
                     /translation="MTVKRLGVLDGWRGISILLVLLGHLFPVGPKAWALNNAVAGAGM
                     AIFFTLSGFLITSVLLADADIRRFLIHRVMRIVPLAWLAMALTLALTRADAALWPPHL
                     LFYANLFPRLLVEGTGHFWSLCVEMQFYMAVALLVATLGRRALFVLPAIAVVVTLYRA
                     GMHYAMDIRTDKRIDEILAGCTLALTWHHRPGWFARGWLAWLPLVLFPLLVASAHAAL
                     PWLNYLRPWVASTMVGCTLGGAAAHPVRRWLSTATLRYIAQISFALYVVHGCLVASWL
                     DTGDKLVKYAKRPLFIAVTWGLAHLSTRYYEAFFIRLGKRWAGK"
     gene            complement(3410..>4322)
                     /locus_tag="VIN75_16635"
     CDS             complement(3410..>4322)
                     /locus_tag="VIN75_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY8975847.1"
                     /translation="QDHPKDIKGNNEMLQLVKPEVLREIHSQYLAAGADIIETNTFGA
                     TSVAQEDYGLALLAREMNVAAARIARECADQYSSSDKPRFVAGALGPTPRTASISPDV
                     NDPGARNTSFDELKAAYKEQAEGLVEGGADLFLVETIFDTLNAKAAIFALDELMDETG
                     VRLPVIISGTVTDASGRILSGQTVTAFWHSVRHARPLAVGLNCALGAALMRPYIEELA
                     KVAGDTAVSCYPNAGLPNPMSDTGFDETPEVTGALIEEFAQAGFLNIAGGCCGTTPEH
                     IAAIARRVGAYRPRSVHDPLFSPLLAA"
BASE COUNT          639 a         1520 c         1529 g          634 t
ORIGIN      
        1 gcgcaggacg ggatcgaggt cgtcgtacac cccgaccatg ccggcgttga cgattcccat
       61 gtccatcccg gcgcggatgg cgtggtacag gaacaccgtg tggatcgcct cgcgcaccgg
      121 ctcgttgccg cggaacgaga agctcacgtt cgacacgccg cccgagacct tcgcgccggg
      181 caggttgcgc ttgatccagc gcgtggcctc gatgaagtcg acggcgtagt tgtcgtgctc
      241 ctcgatgccg gtggcgatcg cgaagatgtt cgggtcgaag atgatgtcct ccggcgggaa
      301 gcccacctcg tcgaccagca cgcggtacgc gcgttcgcag atctcgatct tgcgctcgaa
      361 cgtgtcggcc tggcccttct cgtcgaacgc catcacgacg gccgcggcgc cgtagcggcg
      421 caccaggcgt gcctggcggc ggaactcgtc ctcgccttcc ttcatcgaga tcgagttgac
      481 gatgcccttg ccctgcaggc atttcaggcc ggcctcgatc acgctccact tggacgagtc
      541 caccatcacg ggcacgcggg cgatctcggg ctcgctggcc accaggttca ggaagcgcac
      601 catcgccgcc tggctgtcca gcatcgcctc gtccatgttg acgtcgatga tctgggcgcc
      661 gttctcgacc tgctggcgcg ccacggcgag cgcctgctcg aactcgccgg ccaggatcag
      721 gcgggcgaag gccttggagc cggtgacgtt ggtgcgttcg ccgatgttga cgaacaggtg
      781 gccggcgtcc agggtgacgg gctcgagccc ggacagcttc atcggaggcg tgccggaagc
      841 ggtgttttct gcgggggaca tgtgccaaat tgtaggggtc ggcactcaag cagacccttc
      901 gcaaggccga tatgccggtt ctgacaaccc gtttcggctg ccccgtttct ccatgaaggt
      961 cgttcccctg ccctccgaag gcctgcgcat cggccgtccg ctcccgttca cggtgcgcga
     1021 cggcttcggg gccgtcctgc tggcccgggg atcgaccatc cagaccgaga agcagctgca
     1081 actgctgcgg tcccgcgaga tcttcgtgga catgtcggac atggaatcgg tccagcgcat
     1141 gatcaacggc cagctcgacc agatgttccg ccaggacgtc gccctcggcc gcatcgccgc
     1201 cgcgcggccc gatttcgagg gcatcccgct gcccggccag gccgcccggc ccgccgaacg
     1261 gccgctggac tggcccaacc tgcagatgcg cctgcgcctg ctgctgtccg acccgcgcgc
     1321 cgccgactgg gtcgcccggc tgcgcacgct gcgcgacgac gtgctggcgc tggtcgaacg
     1381 cggccccgac cgcgcgctga tgcgcctgat gtacgacgcc agccacgact tccaggacta
     1441 cagcgccaac cacagcctgt tcgtgtgcgt tctggcgtcg atggcctgca cgcagttgcc
     1501 cggctggcag gccgattggg cggacgcgct gacgctcgcg tcgctgtcga tgaacgtgag
     1561 cttcacgcag atgcaggacg agctggcgcg ccagtccggc ccgatgagcg acgcccagcg
     1621 cgacctgatg cacgagcacg cggcgcgcgc cgccgacctg ctggccgagg tcggcatcga
     1681 cgatccgatc tggctgcacg ccgtgcgcca ccaccacagc gcgccggccg gcccgatggc
     1741 cggtcgctcg ccgcaggaga cggtggcccg cctgatccgt cgcgccgacc gctacgcggc
     1801 gcgcctgtcg ccgcgcaaga gccggcccgc ctcctcggcc accgccgcgg cccaggccgc
     1861 attgctggac gagggcggcc acaccgacga ggccggccag gcgctgatcc gcacgctggg
     1921 cctgtacccg ccgggcgtgt gggtgaagct gcagtgcggc gagatcgcga tggtggtgcg
     1981 ccgcacgccg atcgccaagt cgcccgtcgt cgtgagcctg gtgggacgca gcggcatgcc
     2041 gctggcggtg cccgccacgc gcaacaccaa gctgccggac tacgccgtca ccggcgccgt
     2101 gccgcccggc caggtcaacg tgcggccgaa cctggacgtg ctcgagaagc tcggctgagc
     2161 cgtcctcact tgcccgccca gcgcttgccc aggcggatga agaaggcctc gtagtagcgc
     2221 gtggacaggt gggccaggcc ccaggtcacc gcgatgaaca gcgggcgctt ggcgtacttg
     2281 acgagcttgt cgccggtgtc cagccagctc gcgaccaggc agccgtgcac cacgtacagc
     2341 gcgaacgaga tctgcgcgat gtagcgcagc gtggccgtcg acagccagcg ccgcaccggg
     2401 tgcgccgccg caccgcccag cgtgcagccc accatcgtcg acgcgaccca cggcctcagg
     2461 tagttcagcc acggcagcgc ggcgtgcgcg gaggccacca gcagcgggaa cagcaccagc
     2521 ggcagccagg ccagccaacc gcgcgcgaac cacccgggcc ggtggtgcca cgtgagcgcg
     2581 agcgtgcagc cggccaggat ctcgtcgatg cgcttgtcgg tgcggatgtc catcgcgtag
     2641 tgcatgcccg cgcggtacag cgtcacgacc acggcgatgg cgggcagcac gaacagcgca
     2701 cgccggccca gcgtcgccac gagcagcgcc acggccatgt agaactgcat ctcgacgcac
     2761 aggctccaga agtggccggt gccctcgacc agcaagcgcg ggaacaggtt ggcgtagaac
     2821 agcaggtgcg gcggccacag cgccgcgtcg gcccgggtca gcgccagcgt cagcgccatc
     2881 gccagccacg cgagcggcac gatgcgcatg acgcggtgga tcaggaagcg gcggatgtcg
     2941 gcgtcggcca gcagcacgct ggtgatcagg aagcccgaca gcgtgaagaa gatcgccatg
     3001 cccgcgcccg cgacggcgtt gttcagggcc caggccttcg gcccgaccgg gaacaggtgg
     3061 cccagcagca ccagcaggat gctgatgccg cgccagccgt ccaggacgcc gagtcgtttc
     3121 accgtcacct tgccgctccc tccgcgctgt tcttgccgat gcacgcgccg caggcgggcg
     3181 ccaggacgcg cggcacggcc tcgggccgtt ccgtcgcgag cggcggatca gcaggaatcg
     3241 gacccggccc ggcggccggc agcgcggcga tggcggcgcg gcatgcagga ggccgttcga
     3301 cgcccggtgg cgcgtccgcc gcgtcgcgca gtcagcggcg cgcgcctgcg ccgggcaaaa
     3361 ggaaagggcg cccgcgagcg ccctctttta cccgtggcct tgcggcaaat caggcggcca
     3421 gcagtggcga gaacagcggg tcgtgcacgc tgcgcgggcg gtacgcgccg acgcgtcgcg
     3481 cgatggcggc gatgtgctcc ggcgtcgtcc cgcagcagcc gccggcgatg ttcaggaagc
     3541 ccgcctgcgc gaattcctcg atcagcgcgc cggtgacctc gggcgtctcg tcgaagccgg
     3601 tgtcgctcat cgggttgggc aggccggcgt tcgggtaaca gctgacggcg gtgtcgccgg
     3661 ccaccttcgc cagttcctcg atgtacggcc gcatcagcgc cgcgccgagc gcgcagttca
     3721 ggccgacggc gagcgggcgc gcgtggcgca ccgagtgcca gaacgcggtg acggtctgcc
     3781 cggacaggat gcggcccgag gcgtcggtga cggtgcccga gatgatgacg ggcaggcgca
     3841 cgccggtctc gtccatcagc tcgtcgagcg cgaagatggc cgccttggcg ttgagcgtgt
     3901 cgaagatcgt ctcgaccagg aacaggtcgg cgccgccttc gaccaggccc tcggcctgtt
     3961 ccttgtaggc cgccttgagc tcgtcgaagc tggtgttgcg cgcgcccggg tcgttgacgt
     4021 cggggctgat gctggcggtg cgcggcgtcg ggccgagcgc gccggcgacg aagcgaggct
     4081 tgtcggacga cgaatactgg tcggcgcatt cgcgggcgat gcgcgccgcg gcgacgttca
     4141 tctcgcgcgc caggagcgcg aggccgtagt cctcctgcgc caccgaggtg gcgccgaagg
     4201 tgttggtctc gatgatgtcg gcgccggcgg ccaggtactg cgagtggatc tcgcgcagca
     4261 cctcgggctt gaccagctgc agcatctcgt tgttgccctt gatgtccttg ggatggtcct
     4321 gg
//
LOCUS       DATCRO010000614         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_456872, whole genome shotgun sequence.
ACCESSION   DATCRO010000614 DATCRO010000000
VERSION     DATCRO010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456872"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIN75_16640"
     CDS             <1..198
                     /locus_tag="VIN75_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975848.1"
                     /translation="LVKNVPVMTLELKNARVVPPQELAAMWNDLQRWIREQMTNPGVQ
                     ASAQRSPVPGTRVIGAPRRVD"
     gene            288..854
                     /locus_tag="VIN75_16645"
     CDS             288..854
                     /locus_tag="VIN75_16645"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HEY8975849.1"
                     /translation="MNARHRLLVACAVALPAVAAMAQQPHIRPGLWENTVSVKSDNAQ
                     MNAAMEQMKQRMAAMTPEQRAAMEKAMGGHAAMNPGGPTTMRVCVTKEQIARGFHPEE
                     RGHCARTNISSSGNVTKFDFACQSERGGTVTGHGVYTELSDTAYTVSTAADMVSPKMT
                     MHIQNDTSGKFISSDCGDVKPVEAPPAR"
     gene            complement(877..1281)
                     /locus_tag="VIN75_16650"
     CDS             complement(877..1281)
                     /locus_tag="VIN75_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975850.1"
                     /translation="MTRSRPDDDAAFDDFLRGSAPKPLADHGFTARTMAAVERATRAQ
                     PVRRRAAPPAPLAIARALAIEQQLHDAQARRWRWATAGVVAGYLLLVLAVWLSPEGSV
                     AAMLPTPGEVFPVGLLLAAGAAWVAVRELRAA"
     gene            complement(1278..1859)
                     /locus_tag="VIN75_16655"
     CDS             complement(1278..1859)
                     /locus_tag="VIN75_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8975851.1"
                     /translation="MATSPVVSDESAREQAQAADWLARARRGDRGAFARLVETHQSRV
                     RLQLRRLTRDDAALADDLAQETFVQAWLHLADFRGDARLATWLHRIALTRFLQHARRP
                     QLPVEWQGDDVPEAGHDPRPAEGLGRDVERALQALTEVQRLAVVHCFHLDLSHAEAAQ
                     VLGLPLGTLKSHIDRAKTRLRELLQAWNPESAT"
     gene            complement(1869..2270)
                     /locus_tag="VIN75_16660"
     CDS             complement(1869..2270)
                     /locus_tag="VIN75_16660"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEY8975852.1"
                     /translation="MDPWSRLFTPELLIPVCGILMPLGVAIAALNFAWKYQERKHKTI
                     VDLLEKGLPVPRELLRAPQRNGSPLMRALTLLGVGVGTCGFLGAMFRWDHGLWAAGLI
                     PLCIGVAQLIAIRLEPPRPPEPPEPSSIDLQ"
     gene            complement(2349..2984)
                     /locus_tag="VIN75_16665"
     CDS             complement(2349..2984)
                     /locus_tag="VIN75_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8975853.1"
                     /translation="MATSRTSARDPRVDAYIAQAAPFAQAPLAHIRDAMHAALPDVTE
                     AIKWSHPFFLLEGRPFANMAAFKAHCSLGFWRGGRPVAEEAAGDREKAMGQFGRIESV
                     ADLPKAAALRKLIVEARAAWTAAVADKASAPPAPKAKRAAPVVPDDLAAALRAAAGAR
                     RRYDAFTPSQQAEYVDWIVEAKREATRASRVAQAVEWIAEGKTRNWKYQNC"
     gene            3148..>3527
                     /locus_tag="VIN75_16670"
     CDS             3148..>3527
                     /locus_tag="VIN75_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975854.1"
                     /translation="MRFQNILAGALLAMLAACGGGGGGGSASDPSGKVTISAVGPSGP
                     VGSGGQTQFDVTVRNTSGSAVANVVAQLSLGAGLTRTGVVCTAANGATCPADASAMAI
                     ASLPAGGSVHFVVLATVAAGTVAST"
BASE COUNT          504 a         1287 c         1290 g          446 t
ORIGIN      
        1 ctcgtgaaga acgtgcccgt gatgacgctg gagctgaaga acgcgcgcgt cgtcccgccg
       61 caggaactgg cggcgatgtg gaacgacctg cagcgctgga tccgcgagca gatgaccaac
      121 cccggggtgc aggcgagcgc gcagcgcagc cccgtgccgg gcacgcgcgt gatcggcgcc
      181 ccgcgccgcg tcgactgatc cttcccccga atcgggggtg gtggcagcct gggacggggc
      241 gcagaatccg cgccactcgt catcgacgcc acctcgaggg atccgccatg aacgctcgcc
      301 atcgccttct cgtcgcctgc gccgtcgcgc tgcctgccgt cgcggccatg gcgcagcagc
      361 cgcacatccg gccagggctg tgggagaaca cggtcagcgt caagagcgac aacgcccaga
      421 tgaacgccgc catggaacag atgaagcagc gcatggccgc gatgacgccc gagcaacgcg
      481 ccgcgatgga aaaggccatg ggtggtcacg ccgccatgaa cccgggcggc ccgaccacga
      541 tgcgcgtgtg cgtcaccaag gagcagatcg cccgcggctt ccaccccgag gagcgcggcc
      601 actgcgcgcg gacgaacatc agcagctcgg gcaacgtgac gaagttcgac ttcgcctgcc
      661 agtccgagcg cggcggcacg gtcaccgggc acggcgtcta cacggagctg agcgacaccg
      721 cgtacacggt gagcacggcc gccgacatgg tcagcccgaa gatgaccatg cacatccaga
      781 acgacacgtc gggcaagttc atctcgagcg actgcggcga cgtcaagccg gtcgaggccc
      841 ccccggcgcg ctgaggcccg gcgcgcggcg gacgcttcag gccgcgcgca gctcccgcac
      901 cgcgacccac gccgcgccgg ccgccagcag cagccccacc gggaagactt cgcccggcgt
      961 cggcagcatc gccgccacgc tgccctcggg cgacagccac accgccagca ccagcagcag
     1021 gtagcccgcg accacgcccg ccgtcgccca gcgccagcgc cgcgcctgcg cgtcgtgcaa
     1081 ctgctgctcg atggccagcg cgcgcgcgat cgccagcggg gcgggcggcg cggcgcggcg
     1141 ccgcaccggc tgcgcgcgcg tggcgcgttc caccgcggcc atcgtgcgcg ccgtgaagcc
     1201 gtggtcggcc aggggcttgg gcgcgctgcc gcgcaggaag tcgtcgaagg cggcatcgtc
     1261 gtcgggtcgg gatcgggtca tgtggcggac tccgggttcc aggcctgcag cagctcgcgc
     1321 agccgcgtct tggcccggtc gatgtgggac ttcagcgttc ccagcggcaa gccgagcacc
     1381 tgcgcggcct cggcgtgcga caggtcgaga tggaagcagt gcacgacggc cagccgctgg
     1441 acctcggtca gcgcctgcaa tgcgcgctcc acgtcgcggc ccaggccctc ggcggggcgt
     1501 gggtcgtgcc cggcctcggg cacgtcgtcg ccctgccatt cgaccggcag ctgcggccgg
     1561 cgcgcgtgct gcaggaagcg cgtcagcgcg atgcggtgca gccaggtggc caggcgcgca
     1621 tcgccgcgga agtcggccag gtgcagccag gcctgcacga aggtctcctg cgcgaggtcg
     1681 tcggccagcg ccgcgtcgtc gcgcgtcagc cggcgcagct gcagccgcac gcgcgactgg
     1741 tgggtctcga ccagacgcgc gaacgcgccg cggtcgccgc ggcgcgcgcg ggcaagccag
     1801 tctgcggcct gcgcctgctc gcgtgcggat tcgtcggaga ccacggggct cgtcgccacg
     1861 tcagcgggtc attgcaggtc gatggacgag ggttcgggcg gctccggcgg gcgaggcggc
     1921 tcgagccgga tcgcgatcag ctgcgccacg ccgatgcaca gcgggatcaa ccccgcggcc
     1981 cacaggccgt gatcccagcg gaacatcgcc cccaggaacc cgcaggtacc cacgccgacg
     2041 cctagcagcg tcagcgcgcg catcagcggc gagccgttgc gctgcggcgc gcgcagcagc
     2101 tcgcgcggca ccggcaggcc cttctcgagc aggtcgacga tcgtcttgtg cttgcgctcc
     2161 tggtacttcc atgcgaagtt gagcgcggcg atggccacgc ccagcggcat gaggatgccg
     2221 cagacgggga tgagcagttc gggggtgaac aggcgggacc agggatccat ggcggtcgtc
     2281 cggagttgat cgatgtccct tggatgcggg cccgaggccg atcggatgca ccccggcccg
     2341 gattcttttc agcagttctg gtacttccag ttccgggtct tgccctcggc gatccattcg
     2401 accgcctgcg cgacgcgcga ggcgcgcgtc gcctcgcgct tggcctcgac gatccagtcc
     2461 acgtactcgg cctgctggct gggcgtgaac gcgtcgtagc gccggcgcgc gccggcggcc
     2521 gcgcgcagcg ccgcggcgag gtcgtcgggc acgaccggcg cggcgcgctt ggccttgggc
     2581 gccggcggcg cgctcgcctt gtcggcgacc gccgcggtcc aggccgcgcg ggcctcgacg
     2641 atcagcttgc gcagcgccgc ggccttgggc aggtcggcca ccgactcgat gcggccgaac
     2701 tggcccatcg ccttctcgcg gtcgccggcg gcctcctcgg cgacgggccg tccgccgcgc
     2761 cagaagccga gcgagcaatg cgccttgaac gcggccatgt tcgcgaacgg ccggccctcc
     2821 agcaggaaga acggatggct ccacttgatg gcctcggtca cgtccggcag cgcggcgtgc
     2881 atcgcgtcgc ggatgtgcgc cagcggcgcc tgcgcgaacg gcgcggcctg ggcgatgtag
     2941 gcgtcgacgc gcgggtcgcg cgcgctggtg cgggacgtgg ccatccgcgc actgtacgcg
     3001 gcggcgtccc gcgacggcaa gctccgcgca agtccggcca cttggccaag cgcgggtctt
     3061 cgcgataatg ctgccgcacc cacggcacgg atagacgccc acgatcgagg cggacgcgca
     3121 cgccggcggg caagggagga agagatcatg cggttccaga acatcctggc gggcgccctg
     3181 ttggcgatgc ttgcagcatg cggcggcggc ggtggaggcg gcagcgcgtc cgaccccagc
     3241 ggcaaggtca ccatctcggc ggtcggtccg tcaggtccgg tgggcagcgg cggccagacg
     3301 caattcgacg tcaccgtccg caacacgtcc ggcagcgccg tcgccaatgt cgtggcccag
     3361 ctgtcgctcg gcgccggcct cacccgcacg ggcgtcgtct gcaccgcggc caacggcgcg
     3421 acctgcccgg ccgacgcctc ggcgatggcc atcgccagcc tgccggccgg cggctccgtg
     3481 catttcgtgg tgctggccac cgtggccgcg ggcacggtgg ccagcac
//
LOCUS       DATCRO010000615        13370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_354976, whole genome shotgun sequence.
ACCESSION   DATCRO010000615 DATCRO010000000
VERSION     DATCRO010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354976"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VIN75_16675"
     CDS             <1..1048
                     /locus_tag="VIN75_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8975855.1"
                     /translation="QKAIAADNAAAAASARTAEAALTTILGVFALAAIGLGSLVGWRV
                     IGSVKRPLADAMSFARRVADGDLSVHVRAERDDELGQLLRALATMQARLRDLVGHIRS
                     TAESIGTASREVAVGNQDLSERTEQTAGNLQNAASAMTQLTGNVRQSAEAAAQANDLA
                     TSATDVARRGAQAVSTVVSTMDEINASSRKISDIIGVIDGIAFQTNILALNAAVEAAR
                     AGEQGRGFAVVASEVRSLAQRSAGAAKEIKSLIGASVDRVETGARQVADAGATMREIE
                     SSVERVTAIISEISAAAAEQSSGIVQVGGSVQHLETMTQQNAALVEQSAAAAESLRGQ
                     AESLNTLVHAFRLE"
     gene            1180..1350
                     /locus_tag="VIN75_16680"
     CDS             1180..1350
                     /locus_tag="VIN75_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975856.1"
                     /translation="MSLLLVFAADSVSCQSMPYQDADSVGEAFGMMMMSIRMSELLSA
                     ECGKRYPDDAAS"
     gene            1423..1635
                     /locus_tag="VIN75_16685"
     CDS             1423..1635
                     /locus_tag="VIN75_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975857.1"
                     /translation="MLDGHPERAGQLDKVASSTLDATIKALAARDAPTQDKVLRDYCA
                     THFRDLASGVWRQRTPRVYEFLRAAP"
     gene            complement(1683..2393)
                     /locus_tag="VIN75_16690"
     CDS             complement(1683..2393)
                     /locus_tag="VIN75_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8975858.1"
                     /translation="MGAQWKEKGKAAVADAKGKLFGKLVKDIMIAARGGADPKDNARL
                     RMTIEAARKVSMPKDTLERAIKKGAGLTGETINYTSVLYEGFAPHRVPVVVECLTDNV
                     NRTASEMRVLFRKGQQGTPGSVTWDYDHVGMVEAEAKDGADPELAAIEAGAQDFEAGD
                     QEGTTLFITDTADLDVVAKALPDHGFVVLSAKLGYKPKNPIDPSSLSAEALEEVESFL
                     QAMDANDDVQNVYAGLAG"
     gene            complement(2559..4376)
                     /locus_tag="VIN75_16695"
     CDS             complement(2559..4376)
                     /locus_tag="VIN75_16695"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY8975859.1"
                     /translation="MDTRTSPARARIARLREVMHRQGVDAVLVPSSDPHLSEYLPGRW
                     QGRQHFSGFTGSMGTLAVTADKAAVFADSRYWTQAEAELRGSGITLVKIPTGTAAHHL
                     DWLASEVRRGGVVAVDGAVLGLAASQALRARLAAAGVSLKTDTDVLADAWDERAELPT
                     ARVYEHAAPHAAQSRADKLAQVRAAMAAVGATHHFVSTVDDIAWITNLRGADVDYNPV
                     FLAHLLIDAHTARLFVGAGKIDAALQAALAADGIGVADYAAAGNALGALPRDAVLLLD
                     PRRITLGFRERVAKGVRVVESINPSTLAKSRKTAEELAFIREAMAQDGAAMCAFYAWF
                     DKATAADRPFGPITEITIDEKLSGERRKRPGFVGLSFPVIAGWNANGAMPHYRATPES
                     HAVIEGDGLLLIDSGGQYVGGTTDITRVWPVGSITSQQRQDYTRVLQGTLALSQARFP
                     RGTLSPMLDALARAPLWAAKLDYGHGTGHGVGYFLNVHEGPQSISKAMTEAAHAMEPG
                     MITSIEPGLYRPGQWGIRIENLVANVAIGASEFGDFLEFETLTLCPIDTRCIEPGMLS
                     ATDVAWLNAYHATVRERLLPLVDGDAKDWLIKRTEPFAG"
     gene            complement(4589..5641)
                     /locus_tag="VIN75_16700"
     CDS             complement(4589..5641)
                     /locus_tag="VIN75_16700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8975860.1"
                     /translation="MDMDEGKLNEFMGKLVVDMGGAAILANVILGEELGLYKAMADGE
                     KVTPEQLAERTGCNARLVREWLSGQAASGYIEHDKGRFRLPEEQAMALAIEDSPVYVA
                     GGASVLSSMYLDKDKLVAAMRGDGALSWADHHPCLFTGTERFFRPGYRAHLVSTWLPA
                     LDGVVEKLEKGAKVADIGCGHGASTVIMAQAFPNSTFLGYDFHDKSIAKARERAAEAG
                     VGDRVKFTKSTAREFPGGDFDLVCFFDCLHDMGDPVGAAHRAHEALKPGGTVLLVEPF
                     ANDELDDNINPVGRMFYAASTCICTPNSLSQEVKLGLGAQAGERRLAHVFHQAGFKHF
                     RRATETPFNLVLEARK"
     gene            complement(5836..6477)
                     /gene="kynB"
                     /locus_tag="VIN75_16705"
     CDS             complement(5836..6477)
                     /gene="kynB"
                     /locus_tag="VIN75_16705"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEY8975861.1"
                     /translation="MTKLWDISPPVDARSPVFPGDTEYHQVWGATIGPGCPVNVSAIT
                     LSPHTGAHADAPLHYEAGAATIGEVPLEPFLGPCRVIHAIASGPLVRMRDLAHALPPA
                     AAPLPERVLVRTYARAPLDQFDPDCAAFAPATVEALADLGVRLVGIDTASVDPASSKT
                     LDSHQVLRRRGLRVLENLVLDEVPEGDYELIALPLRLTSADASPVRAVLRALA"
     gene            complement(6735..8267)
                     /locus_tag="VIN75_16710"
     CDS             complement(6735..8267)
                     /locus_tag="VIN75_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HEY8975862.1"
                     /translation="MKRAGDWKYDIGSMGPPLERGAIVDGFLLEKRLHQGGMASMWRV
                     SRVDAEGKPAPVEGEPPLIMKVPRIKGGEDPATIVGYEVEQMIMPALTGHHVPKYIAR
                     GDFTRQPFIVMEHIAGDTLRPRLAEAPLALDEITEIGIKVANALHDLHRQHVVHLDVK
                     PSNIMIRPDGAAVMVDYGLSRHEHLPDLLEEEFSLPMGTGPYMSPEQVQFIRNDPRSD
                     LFALGVMLYHFTTGERPFGAPTSVRGLRKRLWIDPVPPRALRPDCPPWLQEVILKCLE
                     VQPDKRYQTAAQLALDLQQPDQIVLTRRAERIARSSQWTRLKRWFFALGAEPAEAAAP
                     SATEVLNRSNIIMAAVDVDNASPELLDQLRETVRRIVLTEPGARLACVSVMRIARIGT
                     DALTDHTGKSLHVKQLVGLKHWARPISKSLNLEDGRLTFHVLEAPDPADALVDFAKRN
                     QVDHIVMGARGVSALRRYLGSVSSTVVAESLCTVTVVRASAPDAVPAEPVKQATASDG
                     IS"
     gene            complement(8353..9099)
                     /locus_tag="VIN75_16715"
     CDS             complement(8353..9099)
                     /locus_tag="VIN75_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8975863.1"
                     /translation="MTKIAVVTDLHANREAVEAVFAHARAQGATEWAFLGDFVGYGAD
                     PAWVVDQVREMVDAGAVAVCGNHDEAAVIGSTPQMRPEARMVIDWTRAQLDPGQLDFL
                     AALPFQVQRDDVLYVHANAFAPSQWEYVQGRADAVRSIHATSCHYTFCGHVHEPRLYH
                     LGATGKAGDFSPTPEVAIPLPGYRQWLVIPGSAGQPRDGNPAACYAMFEPDAQRITYH
                     RVPYDHESAAAKIRAAGLPERLAARLAEGV"
     gene            9291..11444
                     /gene="acpA"
                     /locus_tag="VIN75_16720"
     CDS             9291..11444
                     /gene="acpA"
                     /locus_tag="VIN75_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY8975864.1"
                     /translation="MSRHLSLHPLTSALAAAFLAAAAAPAAARAPATGVVLGVDGKVY
                     AHAKVCIDRNHNARCDGNEASVFSGADGGFHLAGHGALVAEVGKGAFLVDTATHDKVA
                     VMRPLVLRSPDVDGATPRAIGPLSTELQAIVDAAGKDGSVAEAERQLRARLGLAEADT
                     ESTRAATIDMLAGQRPRLVAEQESLMQRIAEAVADTGRKDDLAAALASRLDLDRITNV
                     VVIYAENRSFNNLFANFPGATGVPSGKKKGAPAFAPQKDRDGTVLPKLPPAWGGLTAA
                     GQAVTVTQAQTTNVLPNAPFQVDSPTPAWGAPAVTNAIVTRDLVHRFFENQMQIDGGL
                     NDKFAAWGDSGAMVMGWFDGSRTAMWKLARDYTLADRFFQGAFGGSYLNHQYLVCACM
                     PEYPNADTAPAHPSITVLRTDAAGKFLPELALADNSPASALSGPPVFKKSGNLTPKDY
                     LGDGTFHSINTMQPPYQPSYNPPSPDDPSGQYATLAEPTTLPPQTLPTIGDLLTGKGV
                     AWTWYSGGWKAALADRTKVYDDASGDFQAHHQPFNYYAAFDPRTHAADRAAHLKDYDD
                     LVADAAAGRLPPVVFYKPIGKNNEHPGYASLAEGDAHLADTIARLQASPQWAHMLVVV
                     TYDENGGQWDEVAPPKGDLVGPGTRIPAVIISPYARRGFVDHTPYDTGSIARFIAHRW
                     SLPLLPGLKLRDASLEANGLPAMGDLSAALELRPH"
     gene            11458..12774
                     /locus_tag="VIN75_16725"
     CDS             11458..12774
                     /locus_tag="VIN75_16725"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8975865.1"
                     /translation="MRRLAHATAVATLLAGAALAAAGPGAVAPQPRAFGDRPSVEQLT
                     ALGRAMFNDPGLSASGRVSCATCHDPVAAFGPTARTPSPFRDDDPVHDGTRAIPSLRY
                     AQFAGRFTEHTTDDEETHGADGGPTGGLTWDGRADTAREQALIPLFDAREMANADAVS
                     LARRIAAAPYAARFRAAFSPPRGDVLADPDRVVAWTALALEVFEQAPEFAPFDSRYDR
                     WLAGRATLSDAQLRGLALYRDPTKGNCDTCHPSRRSASGRAPLFTDAGFIALAVPRRA
                     ELPPLAASSTSSMQPVAAHARDRDADLGLCRSGRPGLSADPSFCGRFRTPTLRNVALR
                     TSFFHNGSLHSLRDAVAFYATRDTDPARWYARNADGSVHVYDDLPADATPFVHREIPF
                     APRPDGRPRLDERDIDDIVAFLRALTDADMETPAAIAAGVQGLGAR"
     gene            complement(12814..>13370)
                     /locus_tag="VIN75_16730"
     CDS             complement(12814..>13370)
                     /locus_tag="VIN75_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975866.1"
                     /translation="VISGSRFVGLGEGGHLYSSTDGHSWSALTTITGVGTLNAGVFGN
                     GKYVAVGDKGYIAASGDSVAWLAGQVVVVGGNPINLHGITWTGTMFVAVGDNGLITTS
                     KDGSAWTAPATSAVAGPLRSVSASTGGEIVIVGDNGIETSEDGVHWTARNSNGAAALN
                     AVTYASGKFVAVGANSAIKTSSGD"
BASE COUNT         1832 a         4804 c         4821 g         1913 t
ORIGIN      
        1 tcagaaggcc atcgccgccg acaacgccgc cgccgccgcc agcgcgcgca ccgccgaggc
       61 cgcgctgacg accatcctgg gcgtgttcgc gctcgcagcg atcggcctcg gttcgctggt
      121 gggctggcgc gtgatcggca gcgtcaagcg cccgctggcc gacgcgatgt cgttcgcccg
      181 ccgcgtcgcg gacggcgacc tgtccgtcca cgtgcgcgcc gagcgcgacg acgaactggg
      241 ccagttgctg cgggctctgg cgacgatgca ggcgcgcctg cgcgacctcg tcggccacat
      301 ccgcagcacc gccgagtcga tcggcacggc cagccgcgag gtggccgtgg gcaaccagga
      361 cctgagcgag cgcaccgagc agaccgcggg caacctgcag aacgccgcca gcgcgatgac
      421 gcagctgacc ggcaacgtgc gccagagcgc cgaggcggcc gcgcaggcca acgacctggc
      481 gacctcggcc accgacgtcg cccgccgcgg cgcccaggcc gtgtcgaccg tcgtctcgac
      541 gatggacgag atcaacgcct cgtcgcgcaa gatctccgac atcatcggcg tgatcgacgg
      601 catcgcgttc cagaccaaca tcctcgccct gaacgccgcg gtggaagccg cgcgcgccgg
      661 cgagcagggc cgcggcttcg cggtcgtcgc ctccgaggtg cgctcgctcg cgcagcgctc
      721 ggccggcgcg gccaaggaga tcaagtccct gatcggcgcc tcggtcgatc gcgtcgagac
      781 gggggcgcgc caggtcgccg acgccggcgc cacgatgcgc gagatcgagt ccagcgtcga
      841 gcgtgtgacg gccatcatct cggagatcag cgccgccgcc gcggagcagt cctccggcat
      901 cgtgcaggtg ggcggttcgg tgcagcacct ggagaccatg acgcagcaga acgcggcgct
      961 ggtggagcaa agcgccgccg cggccgaatc gctgcgcggc caggccgaga gcctgaacac
     1021 gctggtgcac gcgttccggc tggagtgagt gcacggccgt gacggagtgg ccgactccgc
     1081 cgaccgcgcg cccgtgcgac actttgcgcc cgagcgggtg cgggatcgcc cggcgttcaa
     1141 acccgggtcg agggagtccc cacgctgcgt gccgttgcct tgtcgctgtt gctcgttttc
     1201 gcggccgact cagtgtcgtg ccagtccatg ccgtaccagg acgctgactc cgttggcgag
     1261 gcgttcggca tgatgatgat gtcgatcagg atgtccgaat tgttgtcggc ggaatgcggc
     1321 aagcgctacc cggatgacgc cgcctcgtag gctgcggcga tcacagcttg gcgcagtcac
     1381 gatgcccacg agatcgacgt ttcgtcgcgg cactgtgccc ggatgctcga cggccatccg
     1441 gagcgggcgg gccagctgga caaggtggcg tcgtccacgc tcgatgccac catcaaggca
     1501 ctcgcagcac gcgatgcgcc gacccaagac aaggtgctgc gcgactattg cgccactcac
     1561 ttccgcgatt tggcgtcagg ggtgtggcgg cagcgtacgc cccgggtcta cgagttcctg
     1621 cgcgcagccc cctgacatca tcggcgtgat cgaagcaagg gcggccgcga gccgtcgcgg
     1681 gctcagccgg ccagacccgc gtagacgttc tgcacgtcgt cgttggcgtc catcgcctgc
     1741 aggaagctct cgacttcctc cagcgcctcg gcgctcagcg acgacgggtc gatcgggttc
     1801 ttgggcttgt agccgagctt ggcggacagc acgacgaagc cgtggtcggg cagcgccttg
     1861 gcgacgacgt cgaggtcggc ggtgtcggtg atgaacagcg tggtgccttc ctggtcgccg
     1921 gcctcgaagt cctgcgcgcc ggcctcgatg gcggccagtt ccggatcggc gccgtccttg
     1981 gcctcggcct cgaccatgcc cacgtggtcg tagtcccacg tcaccgagcc gggcgtgccc
     2041 tgctggccct tgcggaacag cacgcgcatc tcggaggcgg tgcgattgac gttgtcggtc
     2101 aggcactcca ccaccaccgg cacgcggtgc ggcgcgaagc cttcgtagag cacgctggtg
     2161 tagttgatcg tctcgccggt caggcccgcg cccttcttga tggcgcgttc cagcgtgtcc
     2221 ttgggcatcg acaccttgcg ggccgcctcg atggtcatgc gcagcctggc gttgtccttc
     2281 gggtcggcgc cgccacgcgc ggcgatcatg atgtccttga cgagcttgcc gaacagcttg
     2341 cccttggcat cagcgactgc cgccttgccc ttttctttcc attgcgcgcc catggcgatc
     2401 tccgtccgtc gaattgctgt tggcccgcgg ggagggcggg catccgcgcc gggcgcgggg
     2461 gtgacagtgt cgcatcccgg gcgatggggg cggcgtgggc gtcgcgtgcg gacgcccggg
     2521 cgtgacgcgc cggctcgtgc ccgcccgcgc gcggcgcctc agcccgcgaa cggctcggtg
     2581 cgcttgatca gccagtcctt cgcatcgccg tccaccagcg gcagcaggcg ctcgcgcacg
     2641 gtggcgtggt aggcattgag ccaggcgacg tcggtggcgc tgagcatgcc cggctcgatg
     2701 cagcgggtgt cgatggggca cagcgtcagc gtctcgaact cgaggaagtc gccgaactcg
     2761 ctcgcgccga tggcgacgtt ggccacgagg ttctcgatgc ggatgcccca ctggcccggg
     2821 cggtacaggc ccggctcgat cgacgtgatc atgcccggct ccatcgcgtg ggccgcctcg
     2881 gtcatggcct tgctgatgct ctgcgggccc tcgtgcacgt tcaggaaata gccgacgccg
     2941 tggccggtac cgtggccgta gtcgagcttg gcggcccaca gcggcgcacg ggccagcgcg
     3001 tcgagcatgg gcgacagcgt gccgcgcggg aagcgcgcct ggctcagcgc cagcgtgccc
     3061 tgcagcacgc gggtgtagtc ctggcgctgc tgcgacgtga tgctgcccac cggccagacg
     3121 cgggtgatgt cggtggtgcc gccgacgtac tggccgcccg agtcgatcag cagcaggccg
     3181 tcgccctcga tcacggcatg cgattcgggc gtggcgcggt agtgcggcat cgcgccgttg
     3241 gcgttccagc cggcgatgac ggggaagctc aggccgacga agcccgggcg cttgcggcgc
     3301 tcgcccgaca gcttctcgtc gatggtgatc tcggtgatcg ggccgaacgg gcggtcggcc
     3361 gcggtggcct tgtcgaacca ggcgtagaac gcgcacatgg cggcgccgtc ctgcgccatc
     3421 gcctcgcgga tgaacgcgag ctcctcggcc gtcttgcggc tcttggccag cgtgctcggg
     3481 ttgatggact cgacgacgcg cacgcccttg gcgacgcgct cgcgaaagcc cagcgtgatg
     3541 cggcgcgggt cgagcagcag caccgcgtcg cgcggcagcg cgcccagggc gttgccggcc
     3601 gcggcgtagt cggccacgcc gatgccgtcg gccgcgagcg cggcctgcag cgcggcgtcg
     3661 atcttgccgg cgccgacgaa caggcgcgcc gtgtgggcgt cgatcagcag gtgggccagg
     3721 aagaccgggt tgtagtcgac gtcggcgccg cgcaggttgg tgatccatgc gatgtcgtcg
     3781 acggtggaga cgaagtggtg cgtcgcgccg accgcggcca tcgcggcgcg cacctgggcc
     3841 agcttgtcgg cgcgcgactg cgcggcgtgc ggcgcggcgt gctcgtagac gcgcgcggtg
     3901 ggcagctcgg cgcgctcgtc ccaggcgtcg gccaggacgt cggtgtcggt cttgagcgag
     3961 acgccggccg ccgccaggcg ggcgcgcagc gcctgcgacg cggccaggcc cagcacggcg
     4021 ccgtcgacgg ccaccacgcc gccgcggcgc acctcgctcg cgagccagtc gaggtggtgg
     4081 gcggcggtgc cggtggggat cttcacgagc gtgatgccgc tgccccgcag ctcggcctcg
     4141 gcctgcgtcc agtagcggct gtcggcgaac accgcggcct tgtcggcggt gacggccagc
     4201 gtccccatcg agccggtgaa gcccgagaag tgctggcggc cctgccagcg gccgggcagg
     4261 tactcggaca ggtgcgggtc gctcgacggc accagcacgg cgtcgacccc ctgccggtgc
     4321 atcacctcgc gcagtcgggc gatgcgggcg cgggcggggg aggtgcgggt gtccatgggc
     4381 ggtctttcgg ttgcggcgcc gcgacgtgcg gatggccgcc gggcagggga gggacgccgg
     4441 gcccggcctc cgcgaggcgg ggcgccgacg tgccgttcgg attgacgatt ttaggcgttg
     4501 cccaccgtgg atgccgggcg tctttccccg tgaaaaccga ggttttcgcg ctcggcggcc
     4561 gctcgccgcc gagcgcgcgg tgcgcaacct acttgcgcgc ttcgagcacg aggttgaacg
     4621 gcgtctcggt ggcgcgccgg aaatgcttga agccggcctg gtggaacacg tgcgccagcc
     4681 ggcgctcgcc cgcctgcgcg cccaggccca gcttgacctc ctgcgacagc gagttgggcg
     4741 tgcagatgca cgtcgacgcc gcgtagaaca tgcggccgac cgggttgatg ttgtcgtcga
     4801 gctcgtcgtt ggcgaacggc tcgaccagca gcaccgtgcc gcccggcttg agcgcctcgt
     4861 gcgcgcggtg cgcggcgccg accgggtcgc ccatgtcgtg caggcagtcg aagaagcaca
     4921 cgaggtcgaa gtcgccgccg gggaattcgc gcgcggtgga cttcgtgaac ttcacgcggt
     4981 cgccgacccc cgcctcggcc gcgcgctcgc gggccttggc gatcgacttg tcgtggaagt
     5041 cgtagcccag gaaggtcgaa ttcgggaacg cctgcgccat gatcacggtc gacgcgccgt
     5101 ggccgcagcc gatgtcggcc accttggcgc ctttctccag cttctcgacc acgccgtcga
     5161 gcgcgggcag ccaggtgctg accaggtgcg cgcggtagcc gggacggaag aagcgctcgg
     5221 tgccggtgaa caggcacgga tggtggtcgg cccaggacag cgcgccgtcg ccgcgcatcg
     5281 cggccaccag cttgtccttg tccaggtaca tgctggacag caccgacgcg ccgcccgcca
     5341 cgtagacggg cgaatcctcg atcgccagcg ccatcgcctg ttcctcgggc aggcggaagc
     5401 ggcccttgtc gtgctcgatg tagccgctgg ccgcctggcc gctgagccac tcgcgcacga
     5461 ggcgggcgtt gcagccggtg cgttcggcga gctgctcggg cgtgaccttc tcgccatcgg
     5521 ccatcgcctt gtacaggccc agttcctcgc ccaggatgac gttggccagg atcgccgcgc
     5581 cccccatgtc gacgacgagc ttgcccatga attcgttcaa cttgccttcg tccatgtcca
     5641 taccctccgc gggctcgtgg actgcgtcgg cgcgaggagc ccgcgcatcg accggcgcgc
     5701 gcacgccaag tgcgctcgaa atcatgcgcc cgctttgcgg cgcgggtcaa cgggaccggc
     5761 gtgccggccc acctgcgttc gtggcccgcg gggcaggggc gatgctatcg gtgggcgggc
     5821 cccgatggcg cgggctcagg ccagcgcgcg cagcaccgcc cgcaccgggc tcgcgtcggc
     5881 gctcgtgagc cgcagcggca gcgcgatcag ctcgtagtcg ccctcgggca cctcgtcgag
     5941 caccaggttc tccagcacgc gcaggccgcg acggcgcagg acctggtggc tgtcgagggt
     6001 cttgctgctg gccgggtcga cgctggcggt gtcgatgccc acgaggcgga cgcccaggtc
     6061 ggccagcgcc tccaccgtgg cgggtgcgaa ggcggcgcag tcggggtcga actggtcgag
     6121 cggcgcgcgc gcgtacgtgc gcaccagcac gcgctcgggc aggggcgcgg ccgcgggtgg
     6181 cagcgcgtgc gcgaggtcgc gcatccgcac cagcgggccg ctggcgatcg cgtggatgac
     6241 gcggcagggg cccaggaagg gctcgagcgg cacctcgccg atcgtcgcgg cgccggcctc
     6301 gtaatgcagc ggcgcgtcgg cgtgcgcgcc ggtgtgcggc gacagcgtga tcgcgctgac
     6361 gttgaccggg cagcccgggc cgatcgtcgc gccccagacc tggtggtatt cggtgtcgcc
     6421 ggggaacacc ggcgagcgcg cgtccacggg cggagagatg tcccagagct tggtcatggg
     6481 gcgagcttac cgcgcgcggc gcttgcgtca cgcggagcgc caagccgtca tcgctgcgcg
     6541 cagcgaagcg gagtcgcagg atcgccttgg cgacgaggcc gcgcggcagg cgtggatcct
     6601 gcgactgcgc gcaggatgaa acctggtgag cgcgatgcgg cgacagcgcg tcaggcggaa
     6661 cggcaagccc gcggctcgcc gcgggcgtgc gaggccaggc cggcgacgca gcgatgctgg
     6721 caacacgccg tcggctacga gatgccatcg gaagccgtcg cctgcttcac cggctccgcg
     6781 ggcaccgcat ccggcgcgct cgcgcgcacc acggtgacgg tgcacagcga ttcggcgacc
     6841 accgtcgacg acacgctgcc caggtaccga cgcagcgccg acaccccccg cgcgcccatc
     6901 acgatgtggt cgacctggtt gcgcttggcg aagtccacca gcgcgtcggc ggggtcgggc
     6961 gcctcgagca cgtggaaggt caaccgcccg tcctcgaggt tgagcgactt gctgatcggc
     7021 cgcgcccagt gcttcaggcc gacgagctgc ttgacgtgca ggctcttgcc ggtgtggtcg
     7081 gtcagcgcgt cggtgccgat gcgcgcgatg cgcatcacgc tgacgcaggc caggcgcgcg
     7141 ccgggctcgg tgagcacgat gcggcgcacc gtctcgcgca gctggtcgag cagctcgggc
     7201 gacgcgttgt cgacgtcgac cgcggccatg atgatgttgc tgcgattgag cacctcggtg
     7261 gccgacggcg cggcggcttc cgcgggctcg gcgccgagcg cgaagaacca gcgcttcagg
     7321 cgcgtccact ggctggagcg ggcgatgcgc tcggcgcgcc gcgtcagcac gatctggtcg
     7381 ggctgctgca ggtccagcgc gagctgcgcg gcggtctggt agcgcttgtc gggctgcacc
     7441 tccaggcact tgaggatgac ctcctgcagc cagggcgggc agtcggggcg cagcgcgcgg
     7501 ggaggcaccg ggtcgatcca caggcgcttg cgcaggccgc gcaccgacgt cggcgcgccg
     7561 aacgggcgct cgccggtggt gaagtggtac agcatcacgc cgagcgcgaa caggtccgag
     7621 cgcgggtcgt tgcggatgaa ctgcacctgc tcgggcgaca tgtacggccc ggtgcccatg
     7681 ggcagcgaga actcctcctc cagcaggtcg ggcaggtgct cgtggcgcga cagcccgtaa
     7741 tcgaccatca cggccgcgcc gtcggggcgg atcatgatgt tgctgggctt gacgtccagg
     7801 tgcacgacgt gctggcgatg caggtcgtgc agcgcgttgg ccaccttgat gccgatctcg
     7861 gtgatctcgt cgagcgcgag cggcgcctcg gccaggcgcg ggcgcagcgt gtcgcccgcg
     7921 atgtgctcca tcacgatgaa gggctggcgc gtgaagtcgc cgcgcgcgat gtacttgggg
     7981 acatggtggc cggtcagcgc cggcatgatc atctgctcga cctcgtagcc gacgatcgtg
     8041 gccggatcct cgccgccctt gatgcgcggc accttcatga tcagcggcgg ctcgccctcc
     8101 accggcgccg gcttgccttc ggcgtcgacg cggctgacgc gccacatgct ggccatgccg
     8161 ccctggtgca ggcgcttctc cagcaggaaa ccgtcgacga tcgcgccgcg ttccagcggc
     8221 gggcccatgc tgccgatgtc gtacttccag tcgccggcgc gcttcatgcg gctgccggcg
     8281 ccggagtcga acgcgccgct ggaacgcgcc gttccggact ggacgggcgc ctttgctgtt
     8341 ggtttgtcca cgctacaccc cctcggcgag acgagcggcc agccgctcgg gcaggccggc
     8401 cgccctgatc ttggcggctg cgctctcgtg gtcgtagggt acacggtgat acgtgatccg
     8461 ttgagcatcc ggctcgaaca tcgcgtagca ggccgcggga ttgccgtcgc gcggctggcc
     8521 ggcggagccg gggatcacca gccactggcg gtagcccggc agcgggatcg cgacctcggg
     8581 cgtgggcgag aagtcgcccg cctttccggt ggcgcccagg tggtacagcc gcggctcgtg
     8641 cacgtggccg cagaaggtgt agtggcagga cgtggcatgg atggagcgca cggcgtcggc
     8701 gcggccctgc acgtattccc actggctggg cgcgaaggcg ttggcgtgca cgtacagcac
     8761 gtcgtcgcgc tgcacctgga acggcagggc ggccaggaag tcgagctggc cggggtccag
     8821 ctgcgcacgc gtccagtcga tgaccatgcg cgcctcggga cgcatctgcg gcgtgctgcc
     8881 gatgacggcc gcctcgtcgt ggttgccgca gacggccacg gcgcccgcgt ccaccatctc
     8941 gcgcacctgg tcgacgaccc aggcgggatc ggcgccatac ccgacgaaat cgcccaggaa
     9001 tgcccactcc gtggcgccct gggcgcgggc gtgggcgaac acggcctcga cggcttcccg
     9061 gttggcatgc aggtcggtca cgaccgcgat cttggtcatc tctcgattca tctctttgcg
     9121 gcggccatgt tgccagcact ccctgacagc ctccgtacgg gattacgcaa gccccaccgc
     9181 gaccagggga cggtggaaac cccgcctcgc gtgacgcctt gaaacggtcg cgcacgaatt
     9241 ggcggcgaga atccgcacgg catccttcac cccaggcaac gagaaccccc atgtcccgac
     9301 acctgagcct ccacccgctg acgtccgcgc tcgccgcggc cttccttgcc gccgcggccg
     9361 cgccggccgc cgccagggcg cccgccacgg gcgtcgtgct gggcgtggac ggcaaggtct
     9421 acgcgcatgc caaggtctgc atcgaccgca accacaacgc gcgctgcgac ggcaacgagg
     9481 cgtcggtgtt ctcgggtgcg gatggcgggt tccatctcgc ggggcacggc gcgctcgtgg
     9541 ccgaggtcgg caagggcgca ttcctcgtcg acacggccac gcacgacaag gtcgccgtga
     9601 tgcgccccct ggtgctgcgc tcgccggacg tcgacggcgc cacgccgcgc gcgatcgggc
     9661 cgctgtcgac ggagctgcag gccatcgtcg acgctgcggg caaggacggc tcggtggccg
     9721 aggccgagcg ccaactgcgc gcgcgcctgg gccttgccga ggccgacacc gagagcaccc
     9781 gcgcggccac catcgacatg ctggccggcc agcgcccgcg tcttgtcgcc gagcaggagt
     9841 cgttgatgca gcgcatcgcc gaagccgtcg ccgacaccgg ccgcaaggac gacctcgccg
     9901 ccgcgctggc gagccggctc gacctcgacc gcatcaccaa cgtcgtcgtc atctacgccg
     9961 agaaccgcag cttcaacaac ctgttcgcca acttccccgg cgccacgggg gtgccgagcg
    10021 gaaagaagaa gggcgcgccg gccttcgcgc cgcagaagga ccgtgacggc accgtgctgc
    10081 ccaagctgcc gccggcctgg ggcggcctga ccgccgccgg ccaggcggtg acggtgacgc
    10141 aggcgcagac caccaacgtg ctgcccaacg cgccgttcca ggtcgactcg cccacgccgg
    10201 cctggggcgc gccggcggtc acgaacgcga tcgtcacgcg cgacctggtc caccgcttct
    10261 tcgagaacca gatgcagatc gacggcggcc tgaacgacaa gttcgccgcg tggggcgact
    10321 cgggcgcgat ggtgatgggc tggttcgacg gcagccgcac ggcgatgtgg aagctggcgc
    10381 gcgactacac gctggccgac cgcttcttcc agggcgcgtt cggcggttcc tatctgaacc
    10441 accagtacct ggtctgcgcc tgcatgcccg agtacccgaa tgccgacacc gcgccggcgc
    10501 atccgtccat caccgtgctc aggaccgacg ccgcgggcaa gttcctgccc gagctcgcgc
    10561 tggccgacaa ctcgcccgct tcggcgctca gcgggccgcc ggtgttcaag aagagcggca
    10621 acctcacgcc caaggactac ctgggcgacg gcacgttcca cagcatcaac acgatgcagc
    10681 cgccgtacca gcccagctac aacccgccgt cgccggacga cccgtcgggc cagtacgcga
    10741 cactggccga gcccaccacg ctgccgccgc agacgctgcc gaccatcggc gacctgctca
    10801 cgggcaaggg cgtcgcgtgg acgtggtact ccggcggctg gaaggccgcg ctggccgacc
    10861 gcaccaaggt ctacgacgac gcgtccggcg acttccaggc gcaccaccag cccttcaact
    10921 actacgccgc cttcgacccc aggacgcacg ccgccgatcg cgccgcgcac ctgaaggact
    10981 acgacgacct cgtggccgac gccgctgccg gcaggctccc gccggtcgtg ttctacaagc
    11041 ccatcggcaa gaacaacgag caccccggct atgcgagcct ggccgagggc gacgcgcacc
    11101 tggccgacac catcgccagg ctgcaggcca gcccgcagtg ggcgcacatg ctggtggtgg
    11161 tcacgtacga cgagaacggc ggccagtggg acgaggtggc gccgcccaag ggcgacctgg
    11221 tcgggccggg cacgcgcatc ccggccgtca tcatctcgcc gtacgcgcgc cgcggcttcg
    11281 tcgaccacac gccgtacgac accggctcca tcgcgcgctt catcgcgcac cgctggtcgc
    11341 tgccgctgct gcctgggctc aagctgcgcg acgcgtcgct cgaggccaac ggcctgcccg
    11401 cgatgggcga cctgagcgcg gcgctcgagc tgcgcccgca ctgacgcgga tcggcgcatg
    11461 aggcggctcg cgcacgcgac cgcggtcgcg acgctgctgg ccggcgccgc gctggccgcg
    11521 gccgggcccg gcgccgtcgc gccgcagccg cgcgccttcg gcgaccggcc cagcgtcgag
    11581 cagctcacgg cgctcggccg cgcgatgttc aacgacccag gcctgtcggc cagcggccgc
    11641 gtctcgtgcg cgacctgcca cgacccggtc gcggccttcg ggcccacggc gcgcacgccc
    11701 agcccgttcc gcgacgacga ccccgtccac gacggcacgc gcgcgatccc gtcgctgcgc
    11761 tacgcccagt tcgccggccg cttcaccgag cacacgaccg acgacgagga gacgcacggt
    11821 gccgacggcg gccccaccgg cggcctcacg tgggacggcc gcgcggacac ggcacgcgag
    11881 caggcgctga tcccgctgtt cgatgcgcgc gagatggcca atgccgacgc cgtgtcgctg
    11941 gcgcgccgca tcgccgccgc accctatgcc gcgcgctttc gcgccgcgtt cagcccgccg
    12001 cgtggcgacg tgcttgccga tcccgatcgc gtcgtcgcgt ggacggcgct cgccctcgag
    12061 gtgttcgagc aggcgcccga gttcgcgccc ttcgacagcc gctacgaccg ctggctggcc
    12121 ggccgcgcga cgctgagcga cgcgcagctg cgcgggctcg cgctgtaccg cgatcccacc
    12181 aaaggcaact gcgacacgtg ccatccgagc cggcgcagcg cgagcggccg cgcgccgctg
    12241 ttcaccgacg ccggcttcat cgcgctggcc gtgccgcggc gcgcggaact gccgccgctg
    12301 gccgcgtcgt cgacgtcgtc gatgcaaccc gtggccgcgc acgcgcgcga ccgcgacgcc
    12361 gatctgggcc tgtgccgatc gggccggccc ggactgagcg cggacccgag cttctgcggg
    12421 cgcttccgca cgcccacgct gcgcaacgtg gcgctgcgca ccagcttctt ccacaacgga
    12481 tcgctgcact cgctgcgcga cgcggtggcc ttctacgcca cgcgcgacac cgaccccgcg
    12541 cgctggtacg cgcgcaacgc cgacggcagc gtgcatgtgt acgacgacct gcccgcggat
    12601 gcgacgccct tcgtccaccg cgagatcccg ttcgcgccgc ggcccgacgg ccgcccgcgg
    12661 ctggacgaac gcgacatcga cgacatcgtc gcgttcctgc gcgcgctgac ggacgcggac
    12721 atggaaacgc cggccgcgat cgcggccggc gtgcaggggt tgggggcccg ctgagcccgg
    12781 tgggacgcgc gcgccccgcc ggacgccggg gcgtcagtcg cccgacgagg tcttgatggc
    12841 gctgttggcg cccaccgcga cgaacttgcc gctcgcatag gtgaccgcgt tcagcgccgc
    12901 ggcgccgttc gaattgcgcg ccgtccagtg cacgccgtcc tcgctggtct cgatgccgtt
    12961 gtcgccgacg atcacgatct cgccgccggt cgaggccgac acggaacgca aggggcccgc
    13021 gaccgcggag gtggccggcg ccgtccacgc cgagccgtcc ttgctggtgg tgatcaggcc
    13081 gttgtcgccg accgcgacga acatcgtgcc cgtccacgtg atgccgtgca ggttgatcgg
    13141 gttgccgccg acgaccacca cctggcccgc gagccaggcg acgctgtcgc cgctggccgc
    13201 gatgtagccc ttgtcgccga ccgcgacgta cttgccgttg ccgaacaccc ccgcgttgag
    13261 cgtgcccacg ccggtgatgg tggtcagcgc cgaccacgaa tggccgtcgg tgctgctgta
    13321 gaggtggccg ccctcgccga ggcccacgaa gcggctgccc gagatgacgc
//
LOCUS       DATCRO010000616         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_130651, whole genome shotgun sequence.
ACCESSION   DATCRO010000616 DATCRO010000000
VERSION     DATCRO010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130651"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="VIN75_16735"
     CDS             <1..2029
                     /locus_tag="VIN75_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY8975867.1"
                     /translation="LLVLSHALWRRGTTIALRPRLAQIGQHLRGTPAAVLGGAALVWI
                     ASGAWIFYNTNVLNHYTTQPERDRRAADFEKTLLPFEKVVQPRVADVTLDVELWPRDA
                     RAVTHGSFALVNRSNEAIPVLHLQWAENLRLDSISFPGATVQTDYPRFHYRIYKLATP
                     MQPGERRVLQFTTTLEQRGFRNDRPMTAVVPNGSFFNNSEVTPSVGFARYGLLEERAK
                     RRKYGLPAELRPPKLEDDAARQFNELTHDSDWVTSDITVTTDGDQTPIAPGQVVSDTG
                     VSKDRDARRTVHFRSDAPINQFFSIQSGRYDVRDAVWHAPAQGDQPAHDVALKVYYAA
                     GHDFNVDRMLGAMGESLALFNKSFSPYQFRQARIIEFPAYASFAQSFANTIPFSEGIG
                     FVQHWTNPTKIDVATYVTAHEIGHQWWGHQLVPSDQQGASMLVETFAQYSALLVMEQH
                     YGKDQVRRFLKYELDRYLRSRGGAVLEELPLYRVEDQDYIYYRKGSVAMYWAKEALGE
                     EVVNRAMRKLLARYAFKGAPYANTTDFLKLLRAEAGPQNEQLISDLFEKITILDLKAT
                     NAAATQRPDGKYEVKFDVQAHKFYADGAGKETEAPMDEDVEVGVFTARPGDKGFGPSD
                     VLLLHKVRVHGPGVPVTVVVDRRPAWVGIDPYNKRIDRNSDDNLTQVGLPR"
     gene            2126..3286
                     /locus_tag="VIN75_16740"
     CDS             2126..3286
                     /locus_tag="VIN75_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8975868.1"
                     /translation="MNGLWVVSVVVHAYVALRLLPGVLAASGMVPAALLAVLLAVSAG
                     MVPLALRRRKGKRTPEEIRRSDMLAWASLTLMGLFSSLFVLTLLRDVVVIVLLLARLA
                     GLRVTDHGLDLSASGVVVAALLVTLAGFYNARKAPRVKTVDIPLKDLPPALQGFKIAQ
                     ISDMHVGPTIKRPAMKYVVAKINQLQVDVVAITGDLVDGNVYELSRHVAPLAGLRSSH
                     GTFFVTGNHEYYSGVHPWLKYLPSLGVRVLLNEHVIILHKHAPIVIAGVTDYSAASYD
                     TSHRTDVALAITGAPRAAVKVLLAHQPRSSTAAEQAGFDLQLSGHTHGGQFLPWNFFV
                     RFQQPFTAGLHRVKDMLIYVSRGTGYWGPPKRFGSPSEITLLRLVAQTERRR"
BASE COUNT          520 a         1203 c         1113 g          487 t
ORIGIN      
        1 gctgctggtg ctgtcgcacg cgctgtggcg ccggggcacg accatcgccc tgcgcccgcg
       61 cctcgcccag atcggccagc acctgcgcgg cacgccggcg gccgtgctgg gcggcgcggc
      121 cctcgtctgg atcgcgtcgg gcgcctggat cttctacaac accaacgtcc tgaaccacta
      181 cacgacgcag cccgagcgcg accgccgcgc cgccgacttc gagaagacgc tgctgccctt
      241 cgagaaggtc gtgcagccgc gcgtggccga cgtcacgctc gacgtggagc tctggccgcg
      301 cgacgcccgc gccgtcacgc acggcagctt cgcgctggtc aatcgcagca acgaggccat
      361 tcccgtgctg catttgcagt gggccgagaa cctgcggctg gactcgatca gcttcccggg
      421 cgcgacggtg cagaccgact acccgcgctt ccactaccgc atctacaagc tcgccacgcc
      481 gatgcagccg ggcgagcgcc gcgtgctgca gttcaccacc acgctcgaac aacgcgggtt
      541 ccgcaacgac cggccgatga cggcggtggt gcccaacggc agcttcttca acaactccga
      601 ggtgacgccg tccgtgggct tcgcgcgcta cgggctgctc gaggagcgcg ccaagcgccg
      661 caagtacggc ctgcccgcgg agctgcgccc gcccaagctc gaggacgacg ccgcgcgcca
      721 gttcaacgag ctcacgcacg acagcgactg ggtgacgtcc gacatcaccg tcaccaccga
      781 cggcgaccag acgccgatcg cgccggggca ggtcgtcagc gacacgggcg tgtccaagga
      841 tcgcgacgcc cgccgcaccg tgcacttccg cagcgacgcg ccgatcaacc agttcttctc
      901 gatccagtcg ggccgctacg acgtccgcga cgcggtgtgg cacgcgccgg cgcagggcga
      961 ccagccggcg cacgacgtcg ccctgaaggt gtactacgcg gccggccacg acttcaacgt
     1021 cgaccgaatg ctcggcgcga tgggcgagtc gctcgcgctg ttcaacaagt cgttctcgcc
     1081 ctaccagttc cgccaggccc gcatcatcga gttcccggcc tatgcgtcgt tcgcgcagtc
     1141 gttcgccaac acgatcccgt tctccgaggg catcggcttc gtgcagcact ggaccaaccc
     1201 gaccaagatc gacgtcgcca cctacgtcac ggcgcacgag atcggccacc agtggtgggg
     1261 ccaccagctg gtgccgtccg accagcaggg cgcctcgatg ctggtcgaga ccttcgcgca
     1321 gtactcggcg ctgctcgtga tggagcagca ctacggcaag gaccaggtgc gccgcttcct
     1381 gaagtacgag ctcgaccgct acctgcgctc gcgcggcggc gccgtgctgg aggagctgcc
     1441 gctgtaccgg gtcgaggacc aggactacat ctactaccgc aagggctcgg tggcgatgta
     1501 ctgggccaag gaggcgctcg gcgaggaagt ggtcaaccgc gcgatgcgca agctgctggc
     1561 gcggtacgcg ttcaagggcg cgccctacgc caacacgacc gacttcctca agctgctgcg
     1621 ggccgaggcc ggaccgcaga acgagcagct gatctccgac ctgttcgaga agatcacgat
     1681 cctcgacctc aaggccacca acgccgcggc cacgcagcgg cccgacggca agtacgaggt
     1741 gaagttcgac gtccaggccc acaagttcta cgcggacggc gccggcaagg agaccgaggc
     1801 cccgatggac gaggacgtcg aggtgggcgt gttcaccgcc cgccccggcg acaagggctt
     1861 cgggccgtcg gacgtcctgc tgctgcacaa ggtgcgcgtc cacgggcccg gcgtgccggt
     1921 cacggtggtc gtcgaccgcc gccccgcctg ggtcggcatc gacccctaca acaagcgcat
     1981 cgaccgcaat tccgacgaca acctgaccca ggtcggcctg cccaggtaaa gcctcgttac
     2041 cgacgacttg gcgccggccg gcccgggtgg acgccgcgat gtgggacgat tgcggtcccg
     2101 gaacgcgggg gctgacgagg gcttgatgaa cgggttgtgg gttgtttcgg tcgtggtgca
     2161 cgcctacgtg gcgctgcgcc tgctgccggg cgtgctggcg gcttccggca tggtgccggc
     2221 ggcgctgctg gccgtgctgc tggccgtctc ggcgggcatg gtgccgctcg cactgcgccg
     2281 gcgcaagggc aagcgcacgc ccgaggagat ccggcgcagc gacatgctgg cctgggccag
     2341 cctcaccctg atgggcctgt tctcgtcgct gttcgtgctg acgctgctgc gcgacgtggt
     2401 cgtcatcgtg ctgctgctgg cgcgcctggc cggcctgcgg gtcacggacc acgggctcga
     2461 cctcagcgcc agcggcgtcg tcgtggccgc gctgctggtg acgctggcgg gcttctacaa
     2521 cgcgcgcaag gcgccgcgcg tcaagaccgt cgacatcccg ctgaaggacc tgccgcccgc
     2581 gctgcagggc ttcaagatcg cgcagatctc ggacatgcac gtcggcccga cgatcaagcg
     2641 cccggcgatg aagtacgtgg tcgcgaagat caaccagctg caggtcgacg tggtggccat
     2701 caccggcgac ctggtggacg gcaacgtcta cgagctgtcg cggcacgtgg cgccgctggc
     2761 cggcctgcgt tccagccacg gcaccttctt cgtcaccggc aaccacgagt actactccgg
     2821 cgtgcacccg tggctgaagt acctgccgtc gctgggcgtg cgcgttctgc tcaacgagca
     2881 cgtgatcatc ctgcacaagc acgcgcccat cgtgatcgcg ggcgtcaccg actattccgc
     2941 ggccagctac gacaccagcc accgcaccga cgtcgcgctg gccatcaccg gcgcgccgcg
     3001 cgcggcggtc aaggtgctgc tggcgcacca gccgcgcagc tcgacggccg ccgagcaggc
     3061 gggcttcgac ctgcagctgt ccggccacac ccatggcggc cagttcctgc cgtggaactt
     3121 cttcgtgcgc ttccagcagc cgttcaccgc gggcctgcac cgcgtcaagg acatgctgat
     3181 ctacgtgagc cgcggcaccg gctactgggg gccgcccaag cgcttcggat cgccgtcgga
     3241 gatcaccctg ctgcgcctgg tggcgcagac cgaacggcgc cgctgacgca cgggccgcgc
     3301 gcggacgtcg cgctgaggta acc
//
LOCUS       DATCRO010000617         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_366640, whole genome shotgun sequence.
ACCESSION   DATCRO010000617 DATCRO010000000
VERSION     DATCRO010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366640"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VIN75_16745"
     CDS             <1..989
                     /locus_tag="VIN75_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975869.1"
                     /translation="TPLATVVLLALFVAACGGGSDGGSSVPAPAPAPPSVVNAELLPQ
                     VIDPAVTTALETHIAINPSPDVAARNRLFVFLPGTGAHPASYQKILASGAAKGLHVLG
                     LNYPNPVEVGVLCAGTAPSEACFWNVRREILTGVDLTTKVAIAPPDAIVTRLAEALAY
                     LQANFPDQGWGRYLLGDGSVDWSKVTVGGHSQGGGHAGVMAKLYAMHGACYFDSPPDW
                     DIVPGQPAGWEAYPNVTPAANQVGFSNTLDTLVPWVQLQPIWQSLGLAAFGAPVSVDS
                     TAAPYGGSHMLTTTTSVAGSTGLHSLTVADLVTPMAGGQPLFDPVWNQACFQ"
     gene            1027..2010
                     /locus_tag="VIN75_16750"
     CDS             1027..2010
                     /locus_tag="VIN75_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975870.1"
                     /translation="MTTLATLALLSSMVAACGGGGSPTPAPAPAPAPAPTPGIAQRAA
                     AAASAASQAADCVAVQPFYWSVGDASGKRGDGSVGIAPPTATTIMSIASASKLVYGAY
                     VAEERGGTLTDTDIHFLNFASGYTDFTSCQPGDTVATCEAAGTNGTYEPANDGKFYYG
                     GGHMEKHAFDNGLAADDDAALATAVNTALGTSFAYSQPQPAGGIYTNAGAYGAFLQRI
                     VGGQLKIGALLGTHPVCTNPHTCASAVYSPIPTESDHYSIGHWVEDDPAAGDGAFSSP
                     GAFGFYPWVDGGKTWWGIVARMAHSSDEQEGVASMKCGRRIRAAWVSAQYP"
     gene            2025..2960
                     /locus_tag="VIN75_16755"
     CDS             2025..2960
                     /locus_tag="VIN75_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975871.1"
                     /translation="MRRKHCLATLLLAAACAAHAAPTPDQRAAAARATAENAADCKAV
                     QPFYWSIGDARGVLADGGAGRRPPSATTPMPVASASKLVYAAFIAQQRQGRLTDDDVR
                     FLHFTSGYVHFRSCRRGQTVGECDAMRENGAHDPAADGKFFYNGGHMQQHAVREGLGN
                     LDDKGLAARINATLGTSFEYMEPQLAGGIRTTAADYGEFLRRIVAGQLQVKSVLGTHP
                     VCTNPGTCPTAAESFIHADVHYSIGHWVEDDADGDGAFSSAGAFGFYPWISRDETTWG
                     VLARFQFTFSGARVGMASLTCGRQIRQAWASGQVR"
     gene            complement(2973..3452)
                     /locus_tag="VIN75_16760"
     CDS             complement(2973..3452)
                     /locus_tag="VIN75_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8975872.1"
                     /translation="MTPKNTICLWYDGDAEAAAHFYAETFPDSKVNAVHKAPGDFPGG
                     KKDNVLTVEFTVLGIPCLGLNGGPMFKHSEAFSFQVATDDQAETDRYWNAIVGNGGQE
                     SACGWCKDKWGLSWQITPRALTDAMKDPDPAVRKRTFDAMMTMGKIDIARIQAARKG"
     gene            3674..4081
                     /locus_tag="VIN75_16765"
     CDS             3674..4081
                     /locus_tag="VIN75_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY8975873.1"
                     /translation="MTKQIFINLPVRDLGKSKAFYAAIGAVNNPQFTDETAACMVWSD
                     TIYVMLLTHAKWKTFTDKPIAAASSSEVSLALAVDSRDAVDTMLAAAGASGGKVDVNP
                     PQDLPFMFSRAFEDLDGHVWEPLFFDMSKFPQG"
     gene            complement(4184..4759)
                     /locus_tag="VIN75_16770"
     CDS             complement(4184..4759)
                     /locus_tag="VIN75_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975874.1"
                     /translation="MDNNTLIAIAVAAVVVIAIIAAVAWSSYQSKRLKTRFGPEYDAV
                     LRRTGSKAKAEKELRQREQRVEKFQIVPLSSADAARFSQSWNRLQGSFVDDPKGVLIE
                     ADKLVRDLLVKRGYPVADFELRAADISVDHPVVVSNYRAAQRIVSLDQRGEATTEDLR
                     KAVVHFRALFDELLGKTTTRDEAVPPQRLAA"
BASE COUNT          767 a         1784 c         1757 g          751 t
ORIGIN      
        1 ccaccccgct tgccaccgtc gtcctgcttg ccttgttcgt cgctgcatgc gggggcggca
       61 gcgacggcgg ttcgtccgtg cccgcgcctg cgcccgcgcc cccgtcggtc gtcaacgccg
      121 agctcctgcc gcaagtgatc gaccccgccg tcaccacggc cctggaaacg cacatcgcga
      181 tcaacccgtc gcccgacgtc gcggccagga accggttgtt cgtgttcctg cccggcaccg
      241 gcgcacatcc ggcgtcgtac cagaagatcc tggccagcgg cgcggcgaag ggcctgcacg
      301 tgctgggcct gaactatccg aacccggtcg aggtcggcgt gctgtgcgcc ggcaccgcgc
      361 cgagcgaggc ctgcttctgg aacgtgcggc gcgagatcct caccggtgtc gacctcacga
      421 ccaaggtcgc catcgcgccg ccggacgcca tcgtgacgcg gctggccgag gcgctggcgt
      481 acctgcaggc caacttcccc gaccagggtt ggggccggta cctgctgggg gacggcagcg
      541 tcgactggag caaggtcacc gtcggcggcc actcgcaggg cggcggccac gcgggcgtga
      601 tggccaagct gtatgcgatg cacggcgcct gctacttcga ttcgccgccg gactgggaca
      661 tcgtgcccgg ccagccggcg ggctgggagg cctacccgaa cgtcacgccg gccgcgaacc
      721 aggtcggttt cagcaacacg ctcgacacgc tcgtgccgtg ggttcaactg cagcccatct
      781 ggcagagcct gggcctggcc gcgttcggcg cgccggtgtc ggtcgattcg acggccgcgc
      841 cgtacggcgg ctcgcacatg ctcaccacca cgacgagcgt ggccggcagc accggcctgc
      901 attcgctgac ggtggccgac ctcgtcacgc cgatggccgg cggccagccg ctgttcgacc
      961 cggtgtggaa ccaggcgtgc ttccaatgac attgccttca cgcacggaga cccgatgatg
     1021 gatcgcatga cgacattggc cacgctggcc ctcctgagct cgatggtggc tgcctgcggc
     1081 ggcgggggct cgccgacccc cgcacccgct cccgcgccgg caccggcccc gacgcccggc
     1141 atcgcccagc gcgccgccgc ggccgcgagc gccgcgtcgc aggccgccga ctgcgtggcc
     1201 gtgcaaccgt tctactggtc ggtcggcgac gcctccggca agcggggcga cggcagcgtc
     1261 ggcatcgcgc cgcccacggc gacgaccatc atgtcgatcg cgtcggcgtc caagctggtc
     1321 tacggcgcgt acgtcgccga ggagcgcggc ggcacgctga ccgacaccga catccacttc
     1381 ctgaactttg ccagcggcta caccgacttc acgagctgcc agccgggcga cacggtggcc
     1441 acctgcgagg ccgcgggcac caacggcacg tacgaaccgg ccaacgacgg caagttctac
     1501 tacggcggcg gccacatgga gaagcacgcc ttcgacaacg gcctggccgc ggacgacgac
     1561 gccgcgctcg cgaccgcggt caacacggcg ctgggcacat cgttcgccta ctcgcagccg
     1621 caaccggccg gcggcatcta caccaacgcg ggcgcgtacg gcgccttcct gcagcgcatc
     1681 gtcggcgggc agctgaagat cggggcgctg ctgggcacgc atccggtctg cacgaatccg
     1741 cacacctgcg caagcgcggt ctactcgccg atcccgaccg agagcgacca ctactcgatc
     1801 ggccactggg tcgaggacga tccggccgcg ggcgacggcg cgttcagcag ccccggcgcg
     1861 ttcggcttct acccgtgggt cgacggcggc aagacgtggt ggggtatcgt cgcccgcatg
     1921 gcgcacagca gcgacgagca ggaaggcgtg gcctcgatga agtgcggccg gcgcatccgc
     1981 gcggcctggg tgtcggcgca atacccgtga gacggagacg agcgatgaga cgcaagcatt
     2041 gcctggcgac actgctgctg gccgcggcct gcgcggcgca cgcggcgccg acgccggacc
     2101 agcgcgccgc cgccgcgcgc gcgaccgcgg agaatgcggc cgactgcaag gccgtccagc
     2161 ccttctactg gtccatcggc gacgcgcgcg gcgtgctcgc cgacggtggc gccggccgga
     2221 ggccgccgtc ggccacgacg cccatgcccg tcgcgtcggc ctccaagctg gtgtacgcgg
     2281 cgttcatcgc gcagcagcgc cagggccggc tgaccgacga cgacgtgcgc ttcctgcatt
     2341 tcacgagcgg ctacgtgcat ttcaggagct gccgccgcgg ccagacggtg ggcgagtgcg
     2401 acgcgatgcg cgagaacggc gcgcacgacc cggccgcgga cggcaagttc ttctacaacg
     2461 gcggccacat gcagcagcac gcggtgcgcg agggcctggg caacctcgac gacaaggggc
     2521 tggccgcccg catcaacgcc acgctgggca cctcgttcga gtacatggag ccgcagctgg
     2581 ccgggggcat ccgcacgacc gcggccgact acggcgaatt cctgcgccgc atcgtggcgg
     2641 gccagctgca ggtgaagtcg gtcctgggca cgcaccccgt gtgcacgaac ccgggaacct
     2701 gcccgaccgc tgccgagtcc ttcatccatg ccgacgtgca ttactcgatc ggccactggg
     2761 tcgaggacga cgcggacggc gacggcgcct tcagcagcgc cggcgccttc ggcttctacc
     2821 cgtggatctc gcgcgacgag acgacctggg gcgtgctcgc gcgcttccag ttcacgttca
     2881 gcggcgcgcg cgtgggcatg gcctcgctga cctgcgggcg ccagatccgc caggcgtggg
     2941 ccagcgggca ggtacgctga ggccgcggcg cgtcaaccct tgcgcgccgc ctggatcctg
     3001 gcgatgtcga tcttgcccat cgtcatcatc gcgtcgaagg tgcgcttgcg cacggcgggg
     3061 tcgggatcct tcatggcgtc ggtgagggcg cgcggggtga tctgccacga caggccccac
     3121 ttgtccttgc accagccgca ggcgctctcc tggccgccgt tgccgacgat ggcattccag
     3181 tagcggtcgg tctcggcctg gtcgtcggtg gcgacctgga acgagaacgc ctccgaatgc
     3241 ttgaacatcg gcccgccgtt caggcccagg cacgggatgc ccagcacggt gaattcgacc
     3301 gtgagcacgt tgtccttctt gccgccgggg aagtcgccgg gcgccttgtg gactgcattg
     3361 accttgctgt ccgggaaggt ctccgcgtag aagtgggcgg ccgcctcggc gtcgccgtcg
     3421 taccagagac agatggtgtt cttgggcgtc atggggttgc tcctgatgtg ttgagggctc
     3481 atgaccgggc gcctggcgcc tcggggagcg gccagcatcg gcgatcgggc ggcggccgtc
     3541 aacgctcgcg agcacgacag gaaaataaat tccggacgtg tcgaaacggc tgtcggtcga
     3601 acgtcgtgtg gacatgccgg cgccgtatcg ccgcatgaac cttccaatcg aaacgacacc
     3661 gaggagtctc accatgacca agcagatctt catcaacctg cccgtgcgcg acctgggcaa
     3721 gtccaaggcc ttctacgcgg cgatcggcgc cgtcaacaac ccgcagttca ccgacgagac
     3781 ggcggcctgc atggtgtggt cggacaccat ctacgtgatg ctgctgaccc acgccaagtg
     3841 gaagacgttc acggacaagc cgatcgccgc ggcgagctcg agcgaggtgt cgctggcgct
     3901 ggccgtcgac agccgcgacg ccgtcgacac gatgctggcc gcggccggtg cgagcggcgg
     3961 caaggtggac gtcaacccgc cgcaggacct gcccttcatg ttcagccggg ccttcgagga
     4021 cctggacggc cacgtctggg agccgctgtt cttcgacatg agcaagttcc cgcaaggctg
     4081 acgccacggg gcggcatgtc gcggcgagcc gatccgccgc gcgggcgccg cgccataccg
     4141 cgccaaggcg tccgggcggc ggcgccacgc gaaccgggat tcctcaggca gcgagccgct
     4201 gcggcgggac ggcctcgtcg cgcgtcgtcg tcttgccgag cagttcgtcg aacagcgcgc
     4261 ggaagtgcac cacggccttg cgcaggtcct cggtggtcgc ttccccgcgc tggtccagcg
     4321 acacgatgcg ctgcgccgcg cggtagttgc tgacgacgac cgggtggtcg accgagatgt
     4381 cggcggcgcg cagctcgaag tccgccaccg ggtagccgcg cttgacgagc aggtcgcgca
     4441 cgagcttgtc ggcctcgatc agcacgccct tggggtcgtc gacgaacgag ccctgcaggc
     4501 ggttccacga ctgcgagaac ctggcggcgt cggccgagga cagcggcacg atctggaact
     4561 tctcgacgcg ctgctcgcgc tggcgcagtt ccttctcggc cttggccttg ctgccggtgc
     4621 ggcgcagcac ggcgtcgtac tcggggccga agcgggtctt cagccgcttg ctctggtacg
     4681 agctccaggc gaccgcggca atgatcgcga tgacgacgac cgcggcgacg gcgatggcga
     4741 tcagggtgtt gttgtccatt tcattctccg gatggatcca gcgatgcccg gcttcggcaa
     4801 gcgacgtacc gcggactctt cggtgcgcaa ccggatgtag cgcgaaagat ggggcactgg
     4861 cgcgccggcc gggagacgcg gggcgaacga ttcgccctgc cggtgcgcaa cggatgggca
     4921 ggggctcggc cttccacttg ctcgtgcagc atgtcgataa tggaatgaaa acttgcaagt
     4981 ttgcctgtcg atctgtagac tcgcccgatg ccgccgcgcc gaccgtcctc ccctcaggcg
     5041 aacgcccacg aactgatgc
//
LOCUS       DATCRO010000618         5964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_378087, whole genome shotgun sequence.
ACCESSION   DATCRO010000618 DATCRO010000000
VERSION     DATCRO010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378087"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VIN75_16775"
     CDS             complement(<1..558)
                     /locus_tag="VIN75_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8975875.1"
                     /translation="MAMSYLPYEPQQQALLPQALQDWLPEGHLAYFISDTVDSLDLQA
                     FYARYEGGGSRNQPFHPAMMVKVLVYAYATGVFSSRKIARKLHEDVAFRVLAAENFPA
                     HRTISDFRAFHLKELGELFVQVVRLAREMGLVKLGTIAVDGTKVKANASRHKAMSYGH
                     MLKVEAELKAQVAVLLQRAKAADEAE"
     gene            complement(662..961)
                     /locus_tag="VIN75_16780"
     CDS             complement(662..961)
                     /locus_tag="VIN75_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY8975876.1"
                     /translation="MTSRRRFISLVPVLGAATLARAQSAPLVDPKDPQAQALGYTADA
                     TKVDKARYPKYAAGQNCGGCALYQGKAGDKAGPCPLYAGKSVLAGAWCSAWVKKA"
     gene            1258..2802
                     /locus_tag="VIN75_16785"
     CDS             1258..2802
                     /locus_tag="VIN75_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8975877.1"
                     /translation="MTSTNRFRLHPLAAATLPVALLIQGCATDPHTGAQSIAGIKVSD
                     DPCAKTATVVGGVIGAVTGAVVTSQFTKSTDARVLGGVAGGAVGAAIGYNLDQRRCAM
                     FAIAKEHNAEITVANVVVPKGDLPPAAAVAANTPSPASATPFTSGSDTTTAGLSVTLK
                     DNGKQFAAGSDKLTPEADALFREVADQYSYPKQLAKLGPTATDDDKAGVESLKTKRIL
                     LVGHTDDTGNTTDNANLSERRAAAVARVFAAEGVPAANLYYQGAGETLPIADNHTDAG
                     RAANRRVEIVDVTDDAAFHKFLATRRQVLAYYRASDAPLPDTPAVSPANAKGKKLPKA
                     NTGFVDFGGVPAATLTSAPDFAAGADATHASRPVVPTCNSDRPRTGNGVKSLATQKDL
                     PLSDYVPGLYNTSWSDTVNGNLIGLTNVDVPRDGGAVSGKPTLLIWKNYNPKKKMPPA
                     DFQANPDVNIYRGSQAILYRVFVGGPMKCMDIWFPYEDTGESRNSNLFYDRQQSLYVS
                     TFELKR"
     gene            2926..3633
                     /locus_tag="VIN75_16790"
     CDS             2926..3633
                     /locus_tag="VIN75_16790"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY8975878.1"
                     /translation="MAACDPATDAVPACPALAAGLWRCRGCAAVCVPPPEGIAQGRTR
                     CPRCDAVVHPRLPDSVARSWAFLLAGTALYLPANLLPVTTTASIGGRQSDTIFSGIVY
                     FWKDGSYGLAALVFVASIVVPMTKLLLLSLLLISLQRHDRRWAVRRLKVWRALEIIGR
                     WSMLDIFAITMLAALVQVESLAELRPGPGAMAFGAVVVLTMLATHWFDPRLLFDKPEG
                     EVAPMPPEFTDPAGDAT"
     gene            3630..5348
                     /locus_tag="VIN75_16795"
     CDS             3630..5348
                     /locus_tag="VIN75_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8975879.1"
                     /translation="MSDPDDPRDEAPAEPAAPAPATPPPVSDLPRPTIARRAHYSLWL
                     VWLVPLVAVVIGIYLGARSILNRGPTVTIYFHNAEGIEANKTHIKYKDVDIGIVRRVK
                     LTKDHREVEVTAEMRGNSGIESMLVTDTRFWVVRPRIGAAGVSGLNTLLSGAYIGMDA
                     GRNATEKREFEGLDVQPAITADVPGRQFTLISDDLGSLDIGSPIYLRRVPVGQVLAYT
                     MLPDGRHIKFTIFVTAPYDRFVTTHSRFWHASGVDVDLGAQGLHVQTQSVVSVLAGGV
                     AFQDLPDPDAPVTLGPPAEAPERTQFTLYEQMSDALKRPDSQGFDYRLLFASSVRGLA
                     VGAPVEYRGLPIGEVTRIAVDGKPGEDNPEPKIAVDVRVYPRRLPTINKSGPEDMTLD
                     DQRKRLDPMIRRGFRAQLKSGSLLTGQLYVSLDFVKDAPVARIDWKTTPPTMPTAPGG
                     LDSLQDSLASLARKLDAIPYDQLSGDLHRNLAQLEVTLRHADDMIQHLNGDVVPEAKD
                     TLADARKAVQDLRKTLGTVDQAVGPQATNALGEVSKAAQSLKALADYLERHPESLLKG
                     KPEDPK"
     gene            5345..>5964
                     /locus_tag="VIN75_16800"
     CDS             5345..>5964
                     /locus_tag="VIN75_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY8975880.1"
                     /translation="MSTTRLALLALLAALAGCAAPPDHFHTLRPALAPASAGAREAAR
                     LLAVGPVTVPDALARDEWVIRTGDTGAMVYDHQLWTQGLGADIAQSLVDYLNRSGLPD
                     GLWADAGPTGSGSGTDLERPAPLRVRVQVLRFDSMLAPAPAVSDQLRWTLECLPSDAT
                     LGTVDAGRYRVVRTAVRDAGGPAPAANAAIDESQRRFDQVARAHADT"
BASE COUNT          913 a         2276 c         1953 g          822 t
ORIGIN      
        1 ctcggcctcg tcggcggcct tggcgcgttg cagcaacacc gcgacctgcg ccttcagctc
       61 ggcttcgacc ttgagcatgt gaccatagct catggccttg tggcggctcg cgttggcctt
      121 gaccttggtg ccgtccaccg cgatcgtccc gagcttgacc aagcccatct cgcgggccaa
      181 gcgcaccacc tgcacgaaca actcgcccag ctccttcaag tggaacgccc ggaagtcgct
      241 gatcgtgcgg tgcgccggaa agttctcggc ggccagcacg cgaaatgcga cgtcctcatg
      301 cagcttccgg gcgatcttgc gcgagctgaa cacgccggtg gcgtacgcgt agaccagcac
      361 cttgaccatc atcgccgggt gaaaaggctg gttgcgcgaa ccgccacctt cgtagcgcgc
      421 gtagaacgcc tgcaggtcca gactgtcgac cgtgtcgctg atgaaatagg ccagatgccc
      481 ttcgggcaac cagtcctgca gcgcctgcgg caataacgcc tgctgctggg gttcgtaggg
      541 cagatagctc atggccaaaa tctactcgcc acaggctcag ccgcgtatcg gtcccagccc
      601 gtagtcggct tctgccgcgc agactcctag ccgtcacgca gccgtgacgg ccggggccgc
      661 ctcaggcctt cttgacccag gcgctgcacc aggcgccagc cagcaccgac ttgccggcgt
      721 acagcgggca cggcccggcc ttgtcgcccg ccttgccctg gtacagcgcg cagccgccgc
      781 agttctggcc ggcggcgtac ttcggatacc tggccttgtc gaccttggtg gcgtcggccg
      841 tgtagcccag cgcctgggcc tgcggatcct tcgggtcgac cagcggggcc gactgggcgc
      901 gcgcgagggt ggcggcgcca agcaccggaa cgagcgagat gaagcggcga cgcgaggtca
      961 tggagcttgc cttccttcca gttggggtgg gtggcccatt gtgcgacggc gcgcggcccg
     1021 ggcaggcggc atgccagccg gcgcggtcgg attcttcgct gccggcccgg ggctgcctgc
     1081 cctggaatcg cccgccgaac caccgggaaa tcccccggaa tgacgagtgc gccgcgccgg
     1141 cagccgtgat tgaataccgg ataggctgcg cggcatccga agggtcgcgc gccgccaggc
     1201 cgtgcacatg ttgcaaaggg acggcctgaa gcacaacacc tgatcgacga gactgccatg
     1261 acttcgacga atcgcttccg cctccacccg ctggcggccg ccacgctccc cgtcgcgctg
     1321 ctgatccagg gctgcgccac cgacccccac accggcgcgc agtccatcgc cggcatcaag
     1381 gtcagcgacg acccgtgcgc caagacggcc accgtcgtgg gcggcgtcat cggggcggtc
     1441 accggcgcgg tcgtcacgtc gcagttcacc aagtccaccg acgcccgcgt gctgggcggc
     1501 gtggccggcg gcgcggtggg cgcggccatc ggctacaacc tggaccagcg ccgctgcgcc
     1561 atgttcgcca tcgccaagga gcacaacgcc gagatcacgg tcgccaacgt cgtcgtgccc
     1621 aagggcgacc tgccgccggc ggccgccgtc gcggccaata cgccctcgcc ggcctccgcc
     1681 accccgttca cctcgggcag cgacaccacc accgccggcc tgtccgtcac gctcaaggac
     1741 aacggcaagc agttcgccgc cggctccgac aagctcacgc ccgaggccga tgcgctgttc
     1801 cgcgaggtgg ccgaccagta ttcatacccg aagcagttgg ccaagctggg ccccacggcc
     1861 accgacgacg acaaggccgg cgtcgaatcg ctcaagacca agcgcatcct gctggtgggc
     1921 cataccgacg acaccggcaa caccaccgac aacgccaacc tgtccgagcg ccgcgccgcc
     1981 gcggtggcac gcgtgttcgc cgccgagggc gtcccggccg ccaacctgta ctaccagggc
     2041 gccggcgaga cgctgccgat cgccgacaac cacaccgatg ccggccgtgc cgccaatcgc
     2101 cgcgtcgaga tcgtcgacgt cacggacgac gccgcgttcc acaagttcct cgccacgcgc
     2161 cgccaggtgc tggcctacta ccgcgcgagc gacgccccgc tgcccgacac gcccgcggtg
     2221 tcgccggcca acgccaaggg caagaagctg cccaaggcca acaccggctt cgtcgacttc
     2281 ggcggcgtgc ccgcggccac gctcacgagc gcgcccgact tcgccgccgg cgccgacgcc
     2341 acgcatgcca gccgcccggt ggtgcccacc tgcaattccg accgcccgcg cacgggcaat
     2401 ggcgtgaagt cgctcgccac gcagaaggac ctgcccctga gcgactacgt gccgggcctg
     2461 tacaacacct cgtggtcgga caccgtcaac ggcaacctga tcggcctgac caacgtcgac
     2521 gtgccgcgag acggcggcgc cgtctccggc aagcccacgc tgctgatctg gaagaactac
     2581 aacccgaaga agaagatgcc cccggccgat ttccaggcca acccggacgt caacatctat
     2641 cgcggcagcc aggccatcct gtatcgcgtc ttcgtcggcg gcccgatgaa gtgcatggac
     2701 atctggttcc cgtacgagga caccggcgag tcgcgcaact cgaacctgtt ctacgaccgg
     2761 cagcagtcgc tgtacgtgag caccttcgag ctcaagcgct gagccgccgc gagccgcacg
     2821 gcggcccggc ctttccgggg cccgttcggc cgccccgcgc gggcgaaccg cttttcgcag
     2881 cgggcgccgt tcttgccctg ccgtcgggct agactccctg cacccatggc cgcctgcgat
     2941 cccgccaccg acgccgtgcc cgcctgcccc gcgctcgcgg ccggactctg gcgttgccgc
     3001 ggctgcgccg cggtctgcgt gccgccgccc gaagggatcg cgcaggggcg cacgcgctgc
     3061 ccgcgctgcg acgcggtcgt gcatccgcgg ctccccgact cggtggcccg cagctgggcc
     3121 ttcctcctcg ccggcacggc gctgtacctg ccggccaacc tgctgcccgt caccaccacg
     3181 gcgtccatcg gcggacgcca gtccgacacg atcttcagcg gcatcgtcta tttctggaag
     3241 gacggctcgt acgggctggc cgcgctcgtc ttcgtggcca gcatcgtggt gccgatgacc
     3301 aagctgctgc tgctgtcgct gctgctgatc tcgctgcagc gccacgaccg ccgctgggcg
     3361 gtgcggcgcc tgaaggtgtg gcgcgcgctc gagatcatcg gccgctggtc gatgctggac
     3421 atcttcgcca tcacgatgct cgccgcgctg gtgcaggtcg agagcctggc cgaactgcgg
     3481 cccggccccg gcgcgatggc gttcggcgcg gtcgtcgtgc tgaccatgct tgccacccac
     3541 tggttcgacc cgcgcctgct gttcgacaag cccgaaggcg aggtcgcgcc gatgccgccc
     3601 gaattcaccg accccgccgg agacgccact tgagcgaccc tgacgacccg cgcgacgagg
     3661 ccccggccga gcccgcggcg cccgcgcccg ccacgccgcc gcccgtgtcc gacctgccgc
     3721 gcccgacgat cgcccgccgc gcgcactact cgctgtggct ggtgtggctc gtgccgctgg
     3781 tggccgtcgt catcggcatc tacctgggcg cgcgctcgat cctgaaccgc ggccccacgg
     3841 tcacgatcta cttccacaac gccgagggca tcgaggccaa caagacgcac atcaagtaca
     3901 aggacgtcga catcggcatc gtgcgccgcg tcaagctcac caaggaccac cgcgaagtcg
     3961 aggtgacggc cgagatgcgc ggcaactccg gcatcgagtc gatgctggtg accgacacgc
     4021 gcttctgggt ggtgcgcccg cgcatcggcg ccgcgggggt cagcggcctg aacacgctgc
     4081 tgtcgggcgc atacatcggc atggacgccg gccgcaacgc cacggagaag cgcgagttcg
     4141 agggcctgga cgtccagccc gcgatcaccg ccgacgtgcc gggccgccag ttcacgctga
     4201 tctccgacga cctgggctcg ctggacatcg gctcgccgat ctacctgcgc cgcgtgcccg
     4261 tcggccaggt gctcgcgtac acgatgctgc cggacggccg ccacatcaag ttcacgatct
     4321 tcgtgaccgc gccctacgac cgcttcgtca ccacgcacag ccgcttctgg catgcgagcg
     4381 gcgtcgacgt cgacctcggg gcgcaggggc tgcacgtgca gacgcagtcg gtggtcagcg
     4441 tgctggccgg cggcgtcgcg ttccaggacc tgcccgatcc cgacgcgccc gtcacgctgg
     4501 ggccgccggc cgaggcgccg gagcgcacgc agttcacgct gtacgagcag atgtccgacg
     4561 cactcaagcg gcccgactcg cagggcttcg actaccggct gctgttcgcg agctcggtgc
     4621 gcggcctcgc cgtgggcgcg ccggtggaat accgcggcct gccgatcggc gaggtcacgc
     4681 gcatcgccgt cgacggcaag cccggcgagg acaaccccga gcccaagatc gccgtcgacg
     4741 tgcgcgtgta cccgcgccgc ctgcccacga tcaacaagtc cgggcccgag gacatgacgc
     4801 tggacgacca gagaaagcgc ctcgacccga tgatcaggcg cggcttccgc gcacagttga
     4861 agagcggcag cctgctcacc gggcagctgt acgtgtcgct ggacttcgtc aaggacgcgc
     4921 ccgtcgcgcg catcgactgg aagacgaccc cgccgacgat gcccaccgcg ccgggcggcc
     4981 tggacagcct gcaggactcg ctcgcctcgc tggccaggaa gctcgacgcg atcccgtacg
     5041 accagctctc cggcgacctg cacaggaacc tcgcgcagct ggaagtcacg ctcaggcacg
     5101 ccgacgacat gatccagcac ctgaacggcg acgtcgtgcc cgaggccaag gacacgctgg
     5161 ccgatgcccg caaggcggtg caggacctgc gcaagacgct gggcaccgtc gaccaggcgg
     5221 tcggtccgca ggccaccaac gccctcggcg aagtctccaa ggccgcgcag tcgctcaagg
     5281 cgctggccga ctacctcgaa cgccatccgg agtcgctcct gaagggcaag ccggaggatc
     5341 cgaaatgagc acgacccgcc tggccctcct cgcgttgctc gccgcgctcg cgggttgcgc
     5401 cgcgccaccc gaccacttcc acacgctgcg cccggccctc gcgcccgcga gcgccggcgc
     5461 gcgcgaggcc gcgcgcctgc tggccgtcgg gccggtgacg gtgcccgacg cgctggcacg
     5521 cgacgagtgg gtcatccgca ccggggacac cggcgccatg gtctacgacc accagctgtg
     5581 gacgcagggc ctgggcgcgg acatcgcgca gtcgctggtc gactacctga accgcagcgg
     5641 cctgcccgac ggcctgtggg ccgacgccgg ccccaccggc tcgggcagcg gcaccgacct
     5701 cgagcgcccc gcgccgctgc gcgtgcgcgt gcaggtgctg cgcttcgact cgatgctcgc
     5761 gccggcgccc gccgtcagcg accagttgcg ctggacgctc gagtgcctgc cgtcggacgc
     5821 caccctcggc acggtggacg ccggccgcta ccgcgtcgtg cgcaccgccg tgcgcgacgc
     5881 cggcgggccg gcgccggcgg ccaacgccgc gatcgacgaa tcgcagcggc gctttgacca
     5941 ggtcgcccgc gcgcacgccg acac
//
LOCUS       DATCRO010000619        17086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_895603, whole genome shotgun sequence.
ACCESSION   DATCRO010000619 DATCRO010000000
VERSION     DATCRO010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895603"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1112..3349)
                     /locus_tag="VIN75_16805"
     CDS             complement(1112..3349)
                     /locus_tag="VIN75_16805"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY8975881.1"
                     /translation="MTTQQPTIIYTLTDEAPLLATHAFLPVVRAFTAPAGIAVEASDI
                     SVAARILGEFPDYLTDEQKVPDNLAELGRLTLLPDTNIIKLPNISASVQQLVAAIKEL
                     QGKGYKVPDYPEQPANDDEKKIRARYAKCLGSAVNPVLREGNSDRRAPLAVKNYARKH
                     PHSMGTWSQASQTHVATMKGGDFYHGEKSMTLDKARDVRMELVTKAGKTTVLKPKVAL
                     QDGEIIDSMFMSKKALLEFYEREMEDARKTGVMFSLHVKATMMKVSHPIVFGHAVRIF
                     YREAFEKHGKLFDELGINVNNGMATLYEKIATLPTSKRDEIIRDLHACHEHRPELAMV
                     DSAKGITNLHAPNDVIVDASMPAMIRIGGKMWGADGRPKDTKAVIPESTFARIYQEVI
                     NFCKTNGAFDPVTMGTIPNVGLMAQQAEEYGSHDKTFEVPEAGEGRIVDIATGEVLLT
                     QHLEEGDIWRMCQVKDAPIRDWVKLAVTRARNSGMPAVFWLDSYRPHEAELRKKVVAY
                     LKDHDTSGLDIRIMSQVRAMRYSLERVIRGKDTISVTGNILRDYLTDLFPIMELGTSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVKQLVEENHLRWDSLGEFLALAVSLEDMGIKL
                     GNAKAKLLARTLDAATGKLLDNNKSPSPKTGELDNRGSQFYLALYWAQELAAQTEDAE
                     LQAHFKPIAAALTSKEKTIVGELSDVQGKPVDIGGYYKPDTDKTNAAMRPSATFNAVI
                     DSAQG"
     gene            complement(3506..5077)
                     /locus_tag="VIN75_16810"
     CDS             complement(3506..5077)
                     /locus_tag="VIN75_16810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8975882.1"
                     /translation="MSTTPPIPERIGKYTISAVLGRGSMGTVYKGFDPHIHRPVAIKT
                     IHRELLGDAQAADSIAARFRNEAKAVGRIQHPGVVAIYDFGEDGDVTFIAMEFVDGRT
                     LDDILAVPQMLSEANVLAVMDQLLEALSVAHAQGVWHRDIKPANLIVTKQGQVKLTDF
                     GIARLEDANLTQVSSMIGTPAYMAPEQFIGKGVDHRADLFACGVLLYRMLTGRRAFTG
                     ATQEVMYKILNEQPPAPSIVTKGMRPAAYDAVVARAIAKDPAERYPDAQAMRAALQGL
                     SRAPAAPGAKATVILPIHRDGEHPGTTSAVSPSVASPHTVPPPRDPLSSHASASNAGH
                     ASHSGHSSHASHSGAASQIGDWDPAELLRLERVLATHVGPMARVMVREAARTHHDAIS
                     LIDAVAHNIPEEARRQKFMAQAHGGNHGGSGSSFGPSRFGSSTFGASTLGSGVKPSTH
                     GGQHAGHAAALTEDFKNHVGQIITRKMGPIARVMVKRAADAAGGSQERFVQLLLDALP
                     EADRWAVQGEINKLG"
     gene            complement(5108..5980)
                     /locus_tag="VIN75_16815"
     CDS             complement(5108..5980)
                     /locus_tag="VIN75_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY8975883.1"
                     /translation="MKLLVKFNLVFLLVFLVGLGVSTFIARGLLRQAAKDQVVDTARL
                     LMTGANGVSTYTATQIKPLLETQMKYTFLPQSIPAYSAAEVLEHLQKTYPDYAFKSAM
                     LNPTNPRDRAVPWEEDIVRTFKQHADLKEFIGERETPTGTALYIARPIQLTNPGCLAC
                     HSTPEAAPKTLTDKYGPANGFGWTLNEPLGAQVVSVPLTLPLQQADRQLMVVVGVLAG
                     VFLLIGAALNLMLYRLVIRPVRTLAGIADRVSLGEFEAPEFAVNSKDEIGVLSDSFNR
                     MRTSLAHAMKMLEG"
     gene            complement(5997..6857)
                     /locus_tag="VIN75_16820"
     CDS             complement(5997..6857)
                     /locus_tag="VIN75_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975884.1"
                     /translation="MPSLSSRLALGAVLAAGAVAASAAPRVPLVTMLDGDATLLRDGA
                     RFALAEGVRLQAGDLLATGHQTRLLRLEFPGGPGIALGPDSRAMVTPNLGESNHAGVY
                     LLTGWAKVAAPAGGPGALRSPVADTDTPAGSLILDVQGGSAEAFAETGPSRVQPRAPD
                     APAQQLKSGERVTVPANGKAVLGRGASPDFVQAMPRAFMDSLPSRLAAFSADVAPRRL
                     GEMSYADAQPWIDAEAPLRRVFAERWRRLAADPQFRHGLVEGLKAHPEWTPILYPPPP
                     PRDHAASSHP"
     gene            complement(6943..7395)
                     /locus_tag="VIN75_16825"
     CDS             complement(6943..7395)
                     /locus_tag="VIN75_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975885.1"
                     /translation="MSQANPRQYIGALRAAASAAKAVNKARAAAYHVLPDGISCKMDW
                     AGWSDPWYCATPRQQALLDVLQDLLDGGETEVPMIALKTALRLEIREPEILRSRDNVG
                     ADGESGYFFDDVLHGLYLLDAQETEQVFYGLIDRLMQPEGVRAGPVER"
     gene            complement(7540..8010)
                     /locus_tag="VIN75_16830"
     CDS             complement(7540..8010)
                     /locus_tag="VIN75_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975886.1"
                     /translation="MKTTVRTAAFALPLLMSTLAMAQLPGRHPHYIHALSDLRAAQWQ
                     IDHRRPEDGEIREDELVASDEVAAAIHSTSSAASSDGKDLGWNPPPDAPAAYDGRLHA
                     AIDLMRAARNDLALPEDDPMARNQQKLAILRVDAALHAAERAIGAVRRLDDSRE"
     gene            8320..9198
                     /locus_tag="VIN75_16835"
     CDS             8320..9198
                     /locus_tag="VIN75_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8975887.1"
                     /translation="MKSPDPAWTGRTSRLVLFHGLASSPKEFGFLTHPLRRHGVRLVA
                     PELPGYSAGTLRDGAAWQDWVDAAGRWLDEFELQSPEPYVLGGLCTGSMLALAAAAAR
                     ARPALRGIALLSPLFAYDGWALPWWYALRPLAYALGLTGLFAMREREPFGLRNERMRA
                     LIRQQIAAGETSLAGPASVPLRVVRESERLSAHVRTLLPELAHPVQVQHARDDEICRL
                     ASVRRALATVPTGLLSLHVLENSYHMITADNDRHLVADRLSDFLQGLDANPFDSVPFA
                     HDISTEFDVVEAAPAP"
     gene            9195..9431
                     /locus_tag="VIN75_16840"
     CDS             9195..9431
                     /locus_tag="VIN75_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8975888.1"
                     /translation="MSTLDRLTHLIHDKFGVDIAAIDADAPFASYNLDSLTVAELMFE
                     IDDAFHVTVPDEAATSVTTLRQLATLIDGLPEKA"
     gene            9431..10645
                     /locus_tag="VIN75_16845"
     CDS             9431..10645
                     /locus_tag="VIN75_16845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8975889.1"
                     /translation="MSSAARIGIAGIGVTCPLGDSLDAAWDRLVSGQGAVGRMAFEGL
                     PDIAAARVTSDVSQVLTRLQQVGTERVSQMALGAVRQALADARIDGWTDPERVGLYLG
                     TGMGGAATMDAGFAALYGGGRIPPLTVPAGMVNGASAVVAIHADIRGPVLTYAVACAS
                     GSVAMAEAAHALRRGEVDIAICGGVEAPLARGTVAAWHALRTLAPPDEADVSRSCRPF
                     AANRAGLVLGEGAAFFVLRREDDLAAPPRAWFAGSAVRCDALHLTNPQVRGQVATLKA
                     ALRAGGLVPSDVGYCNAHGTATVAGDPVECEALRDVWGEHAPTLRVSSTKAAHGHLLG
                     AGGALEAALTVAALQHRVLPATLGVDTLDTRCAGLGHVLGEAQRAPALRHAISNSFAF
                     GGTNAALVFSRA"
     gene            10645..11331
                     /locus_tag="VIN75_16850"
     CDS             10645..11331
                     /locus_tag="VIN75_16850"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY8975890.1"
                     /translation="MRAAGVLLAACAAAAAARADDGAHFVGDLGLGVQRTQASAPGAS
                     ARSFALPYAYGDLGRLFVREDTFGVKLVPSGWGALELVGRVSTEGSDADGAGLAHRTN
                     PRPVGLGTFQETPWGGVFADAFVDTVSGGTLLEASYAAEVPVGPVTLYPQAGLARRSA
                     RYDAHLYDVHAASTTPVLQLSGELPFADTWVALAHVQWEPFDAAVRASPRVSARSRTS
                     ALVALAWRFK"
     gene            11331..12440
                     /locus_tag="VIN75_16855"
     CDS             11331..12440
                     /locus_tag="VIN75_16855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8975891.1"
                     /translation="MDDVILYALPVFGVLMAAEFAWGLARGRNNYGVADTLASLSQGL
                     LSQVVAVCTQFVQVGLYALAYPLLTLTPHSPLWQRWPGVLLAVLLYDFCDYWLHRVSH
                     ESALFWAAHVVHHQSRRFNLSTALRQESAYLLTGWPFFLPLAIVGVPPALFGVAALVV
                     LFYQFWIHTEHVGRLGWLDRWLSTPSNHRVHHATNARYLDRNYGAILVVWDRLFGSFE
                     PESEPCVYGTTVPLAGWAPLHAVSVNYVELWRRFLRVHGWRDRLGVLFRAPGWRAASE
                     PVPGPVAIPADVPLGRGRAVAACALFVLAVACTGGLLWRADTLGPAALAGVAVALVAA
                     LWAIGALLDRRLAPWAAGLAGVASATATLILLRAA"
     gene            12505..13572
                     /locus_tag="VIN75_16860"
     CDS             12505..13572
                     /locus_tag="VIN75_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8975892.1"
                     /translation="MIGLHFGVRVRAALALLGALACAVPSPAADAPAPLHLRIVGGLG
                     SLNQFTRHERPFWETDLARLSQGRYTAEIVPFDRAGIRSQEVLTLVQLGAVPFAALPV
                     SGGITKDPELTAPDLAGLNPDMASMRRSVAAFRPYLETMMRERYGIEVLALYVYPAQV
                     VYCKKPFSGIKGLAGLRVRTSGPAQSDWVEALGGKPVTTGFGDILPFLERDELDCAIT
                     GTMSGNTIGLDKATTHIHPMAISWGLSLFVANGAAWNALPADLQALIRRELPRVEHDI
                     WDESERETADGLACDIGAASCKAGRRGHMAEVPSSPADERLRREIFASTVLPRWLQRC
                     GPQCAAVWNRTIAHVAGIEAR"
     gene            13569..16058
                     /locus_tag="VIN75_16865"
     CDS             13569..16058
                     /locus_tag="VIN75_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8975893.1"
                     /translation="MTPSLRRSSAIVWGATCALLLALVVTAGWLVAAGRRDAIARAEE
                     AVTQTAVASESELNRALLSLDLQLTSLAGVIAPAWRAPGELDAEAAHRAMAAFKDRQL
                     LFTDAALIDDQGHTLAASLPSSDRNGMHLPDGFAATLLAQRVPQLTISDPAISPDTTE
                     PSVYMGRVVALPDGRRALALAEVPVTVLAAIIAQAGGSPALAVTLERGDGQLLATVPS
                     GAGLRPREAAEPVPDEALRGRPVVAPSRLTGVPAIVAARATLYRSLRVTASLPLEDAL
                     QAWRNNRNIILTASVALIALTLLGGAMSQWQFNRLVDARSAAARSVEALDKALVVLPD
                     GFLLCDPDDRVVRWNERFLEMFPWLRGTIRVGMTHAQLVDATRKATEFDATEEAGEQW
                     LDERVRTRRLGEAEWERELANGMVVHITDRRTPDGGMVSLFHDVTAAERRLAQAKENA
                     EAANQAKSQFLATMSHEIRTPLNAVLGLNELMLHSPLDSQQRRHAELIGSSGRLLLAL
                     INDILDVSRIEAGQMQLTSAPFSVRPAAEGVVALMRERAVSKGLALRLDVSPHDDALI
                     EGDVIRIQQILFNLVGNAVKFTDRGVVQVNVTLEPAAGRDVTLRLEVSDTGIGIPESA
                     MATLFDRFTQADSTTMRRYGGSGLGLAITREIVQLMGGSIATASTPGVGSRFTVTIPT
                     RRVERGAPAPDAARSDEARPARPALRLLVAEDNDVNQILINALLTRMGHRVHLVANGR
                     LAVEAVRRGDYDLVLMDLQMPEMDGMEATQAIRALGGAFASLPVIAMTANAFDEDRQA
                     CLAAGMDDYVAKPIDVAHLAEAIARCTATVG"
     gene            16186..>17086
                     /locus_tag="VIN75_16870"
     CDS             16186..>17086
                     /locus_tag="VIN75_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8975894.1"
                     /translation="MLRLELTDISKQYPAVKANDRVRLRVEAGEIHAVLGENGAGKST
                     LMKIIYGATRPDEGEMRWDGRAVEIRNPQEARALGIAMVFQHFSLFDTLTAAENVWLG
                     LDKAWSLADVTKRITEVAHEYGLDVDPLRPVHTLSVGERQRVEIVRALLTKPRLLILD
                     EPTSVLTPQAVQALFVTLRKLAGEGCSILYISHKLDEIRALCHKCTVMRGGRVTGEVD
                     PRQESNAGLSRLMIGAEPPQLEHRQATPGEPVLAVRGLTLARTGAFGADLADVTFEVR
                     RGEILGIAGVSGNGQQELMAALSG"
BASE COUNT         2267 a         6024 c         6205 g         2590 t
ORIGIN      
        1 tcgcttcgcg ctggccggcg ttgcgcttgc gacagggtgg gcaggggctc cgctctccgg
       61 cttgccgtgc gtcggaatcc ggcctggacg gccggattca gcgggttccg tggcttcgac
      121 cgcttcctcg cgctcgccgc ctgagtacgt tcaacgattt ggttggcgga ccgggggctt
      181 ccacccgcgt cgcgccggtc gcgcctgcga tcggaacccc agcccaccct gccgcgccag
      241 cgcgtcagcc ggtggaggaa aacattcgcc attttttagg ggctttggcc tcgacgggcc
      301 atcgaggccg cgtcgaggcc cgcggccagc gctccagccg ggcaccgggc ctgacgcgaa
      361 ttgcgggcct tggtgccggc gctccttctc gtgacagacg aacgccgcgc cttcggcgcc
      421 gcgtgcttgc gttgacgcac agcggctcat gccacagctt gtcgccctgt cgcccggcga
      481 tcgttcaacc gcgcagcgcc gatccacgct cgcggcgccg gaggtgccgc gctcgtcaaa
      541 cacgagaagg agcgccggca ccgaggcccg caattcgcgt caagcccggg gacctgccag
      601 agcgatggcc gcgggcatcg acgcggcctc gatggcccgt cgaggccaaa gcccccttta
      661 aaaactgcga atgttttcag accccggccg aaacggtggc gcggcagggt gggctggggt
      721 tccgatcgca ggcgcgaccg gcgcgacgcg ggtggaggcc cccggtccgc caagcacctc
      781 attgaacgcg ctccgacggc gagcgcgagg aagaagtcga agccgaggaa cccgctgaat
      841 ccggccgtcc aggccggatt ccgacgcacg gcaagccgga gagcggagcc ccagcccagc
      901 ctgtcgcacg tgcaacgccg gccagcgcga agcgacaacg ccgacaacgc cgacaacgcc
      961 gacaacaccg acaacaccga caacaccgac aacaccgaca acagcgacaa cagcgacaac
     1021 gacaacagct cgccaccaaa gaaaacggcc ggatcccgca ggaaccggcc gctcttcaga
     1081 acagcaaaac ggcagtcagc agcgtcgcag gtcagccctg cgcgctatcg atcacggcat
     1141 tgaacgtcgc gctcggccgc atcgccgcat tcgtcttgtc ggtgtccggc ttgtagtagc
     1201 cgccgatgtc gaccggcttg ccctgcacgt ccgacagctc gccgacgatc gtcttttcct
     1261 tcgacgtgag cgccgcggcg atcggcttga agtgggcctg cagttcggcg tcttccgtct
     1321 gcgcggccag ttcctgtgcc cagtacagcg cgaggtagaa ctggctgccg cggttgtcga
     1381 gctcgccggt cttgggcgac ggcgacttgt tgttgtccag cagcttgccc gtggccgcgt
     1441 ccagcgtcct ggcgagcagc ttggccttgg cgttgcccag cttgatgccc atgtcttcca
     1501 gcgacacggc cagcgcgagg aattcgccca gcgagtccca gcgcaggtgg ttctcctcga
     1561 ccagctgctt gacgtgcttg ggcgccgagc cgccggcgcc cgtctcgtac atgccgccgc
     1621 cggccatcag cggcacgatg gacagcatct tggcgctggt gcccagctcc atgatcggga
     1681 acaggtcggt caggtagtcg cgcaggatgt tgccggtgac cgagatggtg tccttgccgc
     1741 ggatcacgcg ctcgagcgag tagcgcatcg cgcgcacctg cgacatgatg cggatgtcca
     1801 ggccggaggt gtcgtggtcc ttcaggtacg cgacgacctt cttgcgcagc tcggcctcgt
     1861 gcgggcggta cgaatcgagc cagaacacgg ccggcatgcc ggagttgcgg gcgcgcgtga
     1921 cggccagctt gacccagtcg cggatcggcg catccttgac ctggcacatg cgccagatgt
     1981 cgccttcctc caggtgctgg gtgagcagca cttcgccggt ggcgatgtcg acgatgcggc
     2041 cttcgccggc ctcgggcacc tcgaaggtct tgtcgtgcga gccgtattcc tcggcctgct
     2101 gcgccatcag gcccacgttg gggatcgtgc ccatcgtgac cgggtcgaag gcgccgttgg
     2161 tcttgcagaa gttgatgacc tcctggtaga tgcgggcgaa cgtgctctcg gggatcacgg
     2221 ccttggtgtc cttcggacgg ccgtcggcgc cccacatctt gccgccgatg cggatcatgg
     2281 caggcatcga cgcgtcgacg atgacgtcgt tgggtgcgtg caggttggtg atgcccttgg
     2341 ccgagtcgac catcgccagt tccgggcggt gctcgtggca ggcgtgcagg tcgcggatga
     2401 tctcgtcgcg cttggacgtg ggcagcgtgg cgatcttctc gtacagcgtg gccatgccgt
     2461 tgttgacgtt gatgcccagt tcgtcgaaca gcttgccgtg cttctcgaag gcctcgcgat
     2521 agaagatgcg cacggcgtgg ccgaacacga tcgggtgcga gaccttcatc atcgtggcct
     2581 tgacgtgcag cgagaacatc acgccggtct tgcgcgcgtc ttccatctcg cgctcgtaga
     2641 actcgagcag ggccttcttg ctcatgaaca tcgagtcgat gatctcgccg tcctgcagcg
     2701 cgaccttcgg cttgagcacg gtcgtcttgc cggccttggt gaccagctcc atgcgcacgt
     2761 cgcgcgcctt gtccagcgtc atcgacttct cgccgtgata gaagtcgccg cccttcatcg
     2821 tggccacgtg cgtctgcgag gcctggctcc aggtgcccat cgagtgcgga tgcttgcgcg
     2881 cgtagttctt gacggccagc ggggcgcggc ggtcggagtt gccttcgcgc agcaccgggt
     2941 tgaccgcgct gcccaggcac ttggcgtagc gggcgcggat cttcttctcg tcgtcattgg
     3001 cgggctgctc ggggtagtcg ggcaccttgt agcccttgcc ctgcagttcc ttgatggccg
     3061 cgaccagctg ctgcaccgac gcgctgatgt tgggcagctt gatgatgttg gtgtcgggca
     3121 gcagcgtcag ccggccgagc tcggccaggt tgtcaggcac cttctgctcg tcggtcaggt
     3181 agtcggggaa ctcgcccagg atccgggccg ccacggagat gtcgctggct tcgacggcga
     3241 tgcccgccgg cgccgtgaag gcgcgcacga cgggcaggaa ggcgtgggtc gccagcaggg
     3301 gcgcctcgtc cgtcagggtg tagatgatgg tcggctgttg ggtggtcatc ggggcggttc
     3361 cagggtttcg aggggcggaa ccgcgacggc cgtgagcgtg cctgcaaggc gtggcgccgg
     3421 ggttcccggc cggcattttc cacccaacgg acaccccgag ggcgccgccc gggctatcaa
     3481 ccgggttaca gccgcgtggc gcccgtcaac ccagcttgtt gatctcgccc tgcaccgccc
     3541 agcggtcggc ctcgggcagc gcatcgagca gcagctggac gaaacgctcc tggctgccgc
     3601 cggcggcatc ggccgcgcgc ttgaccatca cgcgggcgat cggccccatc ttgcgggtga
     3661 tgatctggcc gacgtggttc ttgaagtcct cggtcagcgc cgcggcgtgc ccggcgtgct
     3721 ggccgccgtg cgtcgacggc ttgacgccgc tgccgagggt ggacgcgccg aacgtggacg
     3781 aaccgaacct ggacgggccg aacgaggatc cggacccgcc atggttgccg ccgtgcgcct
     3841 gcgccatgaa cttctggcgc cgggcttcct cggggatgtt gtgcgccacc gcgtcgatca
     3901 ggctgatggc gtcgtggtgc gtgcgcgccg cttcgcgcac catgacgcgc gccatcgggc
     3961 ccacgtgcgt ggcgagcacc cgctccagcc gcagaagctc ggcgggatcc cagtcgccga
     4021 tctgcgaggc ggcgccgctg tggctggcat ggctcgaatg gccggaatga ctggcgtggc
     4081 cggcgttgct cgcgctggcg tgcgacgaga gcggatcgcg cggcggcggc acggtgtgcg
     4141 gcgaggccac gctcggcgag acggcgctgg tggtgcccgg gtgctcgccg tcgcggtgga
     4201 tcggcaggat gacggtggcc ttggccccgg gcgcggccgg cgcgcgcgag aggccctgca
     4261 gcgcggcgcg catggcctgc gcgtcggggt agcgctcggc cggatccttg gcgatggcgc
     4321 gggcgacgac cgcgtcgtag gccgccggcc gcatgccctt ggtcacgatg ctgggcgccg
     4381 gcggctgctc gttgaggatc ttgtacatca cctcctgcgt ggcgccggtg aacgcgcgcc
     4441 gtccggtgag catccggtac agcagcacgc cgcaggcgaa caggtcggcc cggtggtcga
     4501 cgcccttgcc gatgaactgc tcgggcgcca tgtaggccgg cgtgccgatc atcgacgaca
     4561 cctgcgtcag gttggcgtcc tccagccgcg cgatgccgaa gtcggtcagc ttgacctggc
     4621 cctgcttggt gacgatgagg ttggccggct tgatgtcgcg gtgccagacg ccctgcgcgt
     4681 gggcgacgga cagcgcctcg agcagctggt ccatcaccgc cagcacgttg gcctccgaca
     4741 gcatctgcgg caccgcgagg atgtcgtcga gcgttcgccc gtcgacgaac tccatggcga
     4801 tgaacgtgac gtcgccgtcc tcgccgaagt cgtagatggc cacgacgccc gggtgctgga
     4861 tgcggccgac cgccttggcc tcgttgcgga agcgcgcggc gatcgagtcg gccgcctgcg
     4921 cgtcgccgag cagctcgcgg tggatcgtct tgatcgccac cggccggtgg atgtgcgggt
     4981 cgaacccctt gtagacggtg cccatcgagc cgcggcccag cacggccgag atcgtgtact
     5041 tgccgatgcg ttcggggatc ggaggcgtcg tggacatggc gcgggacagg agcaggaacc
     5101 ggaagaatca gccttcgagc atcttcatcg cgtgcgccag gctcgtgcgc atgcggttga
     5161 acgaatcgga cagcacgccg atctcgtcct tggaattgac ggcgaactcg ggcgcctcga
     5221 attcgcccag cgagacgcgg tcggcgatgc cggccagcgt gcgcaccggc cggatgacga
     5281 ggcgatagag catcaggttc agcgcggcgc cgatcagcag gaacacgccg gcgagcacgc
     5341 ccacgaccac catcagctgg cggtcggcct gctgcagcgg cagcgtcagc ggcaccgaga
     5401 cgacctgcgc gcccagcggc tcgttgagcg tccagccgaa gccgttggcg gggccgtact
     5461 tgtcggtcag cgtcttgggc gcggcctcgg gcgtgctgtg gcaggccagg cacccggggt
     5521 tggtcagctg gatcgggcgc gcgatgtaca gcgcggtgcc ggtgggcgtc tcgcgctcgc
     5581 cgatgaattc cttgaggtcg gcgtgctgct tgaacgtgcg cacgatgtcc tcttcccagg
     5641 gcacggcgcg gtcgcgcggg ttggtcgggt tgagcatcgc cgacttgaac gcgtagtcgg
     5701 gataggtctt ctgcaggtgc tcgagcacct cggccgccga gtacgccggg atcgactgcg
     5761 gcaggaaggt gtacttcatc tgcgtctcga gcagcggctt gatctgggtg gccgtgtagg
     5821 tgctgacgcc gttggcgccg gtcatcagca ggcgggcggt gtccacgacc tggtccttgg
     5881 cggcctggcg cagcagcccg cgggcgatga acgtggagac gccgagtccc accaggaaca
     5941 cgagcaggaa caccaggttg aacttgacga ggagcttcat ggcttggcgg acggggtcag
     6001 ggatgggagg aggcggcgtg gtcgcgcgga ggcggcggcg ggtagaggat gggcgtccat
     6061 tccggatgcg ccttcagccc ctcgaccagg ccgtggcgga actgcgggtc ggcggccagg
     6121 cgccgccagc gttcggcgaa cacgcggcgc agcggcgcct cggcgtcgat ccagggctgg
     6181 gcgtcggcgt aggacatctc gcccagccgg cgcggcgcca cgtcggcgct gaacgccgcc
     6241 agccgcgagg gcagcgagtc catgaaggcg cgcggcatcg cctgcacgaa gtcgggcgac
     6301 gcgccgcgcc cgagcacggc cttgccgttg gccgggacgg tgacgcgttc gccggacttg
     6361 agctgctgcg ccggcgcgtc gggcgcccgc ggctgcacgc gcgaggggcc ggtctcggcg
     6421 aaggcctcgg cgctgccgcc ctgaacgtcc aggatcaggc tgccggcggg ggtgtcggtg
     6481 tcggcgaccg ggctgcgcag cgcgccggga ccgccggcgg gcgcggccac cttggcccag
     6541 cccgtgagca ggtagacgcc ggcatggttg ctctcgccca ggttgggcgt gaccatcgcg
     6601 cggctgtcgg ggcccagcgc gatgccgggg ccgccgggga attcaagccg cagcaggcgc
     6661 gtctggtggc cggtggccag caggtcgccg gcctgcaggc gcacgccctc ggcgagcgcg
     6721 aaccgggcgc cgtcacgcag cagcgtggcg tcgccatcga gcatcgtgac caggggcacg
     6781 cgcggcgccg ccgacgccgc gacggcgccg gccgcgagga cggcgcccag ggcgaggcgc
     6841 gaagagagcg acggcatagt cgcggattat ggccaccgcg gggcgggccc ggcgtgactt
     6901 atgcccgatt tcgagggggg ccaccccctg tgcgaaggcg cgtcagcgct cgaccgggcc
     6961 cgcgcggacg ccctcgggct gcatcagccg gtcgatcagg ccgtagaaga cctgctcggt
     7021 ctcctgggcg tccagcaggt acagcccgtg caggacgtcg tcgaagaagt agcccgattc
     7081 gccgtcggcg ccgacgttgt cgcggctgcg caggatctcc ggttcccgga tctccagccg
     7141 cagcgccgtc ttcagcgcga tcatcggcac ctcggtctcg ccgccgtcca ggagatcctg
     7201 cagcacgtcc agcagcgcct gctggcgcgg cgtcgcgcag taccagggat cgctccagcc
     7261 ggcccagtcc atcttgcagc tgatgccgtc ggggagcacg tgatacgccg cggcgcgggc
     7321 tttgttgacc gccttggcgg cggacgcggc ggcgcgcagg gcgccgatgt actgacgtgg
     7381 attggcctga ctcatcggag ccttccgcaa gcgttggaaa agcgggcgcg cggtgcgtgt
     7441 tttcacgcga ttgcgcgctc gccgttccct gtcttatcgg cgcggccccg ggccggcttt
     7501 aacgcggcgg cggcgcgccg cgggccggcc ggacttttgt cactcgcggc tgtcgtccag
     7561 gcggcgcacg gcgccgatgg cgcgctcggc ggcgtgcagc gcggcgtcca cgcgcaggat
     7621 cgcgagcttc tgctggttgc gcgccatcgg gtcgtcctcg ggcagcgcca ggtcgttgcg
     7681 cgcggcgcgc atcaggtcga tggccgcgtg caggcggccg tcatacgccg ccggggcatc
     7741 gggcggcggg ttccagccga ggtccttgcc gtcggacgag gcggcgctcg aggtcgagtg
     7801 gatggcggcg gcgacctcgt cggacgcgac gagctcgtcc tcgcggatct cgccgtcctc
     7861 ggggcggcgg tggtcgatct gccactgcgc cgcgcgcagg tcggacagcg cgtggatgta
     7921 gtgcggatgg cgtcccggca gctgggccat ggccagcgtg gacatcagca gcggcagcgc
     7981 gaacgcggcg gttcggacgg tggtcttcat gctttcctcg atttgtcgtg tgtgagggga
     8041 ccgaggacgc gtcactgcgc ggtcctcgct gcctcaacgt ccacgcgagg gtgcaagccg
     8101 accgcgcgcg cttgccgcaa gctgacgcga tctgtccgat cgacgtgaaa gttaggaaga
     8161 cggcggatcg aggcgctccg ggacgacgcc tcaatcttgc atgagccttg ccattggctg
     8221 aacacgccca tcagggtggc attcgacaat tgaaccgtca cattcaaggc gagtctcatc
     8281 gtcggacgcg tggcagtaca accgttcctg acgtcgacga tgaagtcccc cgatcccgcc
     8341 tggacaggcc gcaccagccg cctcgtcctg ttccatggcc tggccagcag ccccaaggaa
     8401 ttcggctttc tcacccaccc gctgcgccgc cacggcgtgc gcctggtcgc gcccgaactg
     8461 ccgggctaca gcgccggcac gctgcgcgac ggcgcggcct ggcaggactg ggtcgatgcc
     8521 gccggccgct ggctggacga gttcgagctg cagtcgcccg agccctacgt gctgggcggc
     8581 ctgtgcaccg gctcgatgct ggcgctggcc gccgcggccg cgcgcgcgcg gcccgcgctg
     8641 cgcggcatcg cgctgctgtc gccgctgttc gcctacgacg gctgggcgtt gccgtggtgg
     8701 tacgcgctgc ggccgctcgc ctacgccctg ggcctgacgg gcctgttcgc gatgcgcgag
     8761 cgcgagccct tcggccttcg caacgagcgc atgcgcgcgc tgatccgcca gcagatcgcc
     8821 gccggcgaga cctcgctggc cggccccgcc agcgtgccgc tgcgcgtggt gcgcgagagc
     8881 gagcggctgt cggcgcacgt gcgcacgctg ctgcccgagc tcgcgcatcc ggtgcaggtg
     8941 cagcacgcgc gcgacgacga gatctgccgc ctcgccagcg tgcggcgcgc gctcgcgacc
     9001 gtgcccaccg gcctgctgag cctgcacgtg ctggagaaca gctaccacat gatcacggcg
     9061 gacaacgacc gccacctggt cgccgaccgc ctcagcgatt tcctgcaggg cctcgacgcc
     9121 aaccccttcg acagcgtgcc gttcgcgcac gacatctcca ccgaattcga cgtcgtggaa
     9181 gcggcgcccg caccatgagc accctcgacc gcctcaccca cctgatccac gacaagttcg
     9241 gcgtggacat cgccgccatc gacgccgacg cgccgttcgc gagctacaac ctcgattcgc
     9301 tgaccgtcgc cgagctgatg ttcgagatcg acgacgcgtt ccacgtcacc gtgcccgacg
     9361 aggccgccac cagcgtgacg acgctgcgcc agctcgccac cctgatcgac gggctgcccg
     9421 agaaggcgtg atgtcgtccg ccgcccgcat cggcatcgcc ggcatcggcg tcacctgccc
     9481 gctcggcgac tcgctggacg ccgcctggga tcgcctggtc tcgggccagg gcgcggtcgg
     9541 ccgcatggct ttcgaggggc tgcccgacat cgccgcggcc cgggtgacga gcgacgtgtc
     9601 gcaggtgctc acgcgcctgc agcaggtcgg caccgagcgc gtcagccaga tggcgctcgg
     9661 cgcggtgcgc caggcgctgg ccgacgcgcg catcgacggc tggaccgatc cggagcgcgt
     9721 gggcctgtat ctcggcaccg gcatgggcgg tgccgccacg atggacgcgg gcttcgccgc
     9781 gctgtacggc ggcggccgca tcccgccgct gacggtgccg gccggcatgg tcaacggcgc
     9841 gtccgcggtg gtggccatcc acgccgacat ccgcggcccc gtgctgacct atgccgtcgc
     9901 ctgcgcgtcg ggctcggtgg cgatggccga ggccgcgcac gcgctgcgcc gcggcgaggt
     9961 cgacatcgcc atctgcggcg gcgtcgaggc gccgctggcg cgcggcaccg tcgccgcgtg
    10021 gcatgcgctg cgcacgctcg cgccgcccga cgaggccgac gtgtcgcgca gctgccgccc
    10081 gttcgccgcg aaccgcgccg ggctcgtgct gggcgagggc gccgcgttct tcgtgctgcg
    10141 ccgcgaggac gacctggccg cgccgccgcg cgcctggttc gccggctcgg cggtgcgctg
    10201 cgacgcgctc cacctgacca atccgcaggt gcgcggccag gtcgcgacgc tcaaggcggc
    10261 gctgcgggcc ggcggactcg tgccgtcgga cgtgggttac tgcaacgcgc acggcaccgc
    10321 cacggtcgcc ggcgaccccg tcgaatgcga ggccctgcgc gacgtgtggg gcgagcacgc
    10381 gccgacgctg cgcgtgagct ccaccaaggc ggcacacggc cacctgctgg gcgcgggcgg
    10441 ggcgctcgag gccgcgctga cggtggccgc cctgcagcac cgcgtgctgc cggccacgct
    10501 gggcgtcgac acgctcgaca cgcgctgcgc gggcctgggc cacgtgctgg gcgaggcgca
    10561 gcgcgcgccg gcgctgcgcc acgcgatctc caactcgttc gcgttcggcg gcaccaacgc
    10621 ggcgctcgtg ttcagccgcg cgtgatgcgg gccgccggcg tgctgctcgc ggcctgcgcg
    10681 gccgccgccg cggcgcgggc cgatgacggc gcgcacttcg tcggtgacct cggcctgggc
    10741 gtgcagcgca cgcaggcgtc cgcgcccggc gcgtcggcgc gcagcttcgc gctgccctac
    10801 gcgtacggcg acctcggccg gctgttcgtg cgcgaggaca ccttcggcgt gaagctcgtg
    10861 ccctccggct ggggcgccct cgagctcgtc ggccgcgtca gcaccgaagg cagcgacgcc
    10921 gacggcgcgg gcctcgcgca tcgcacgaac ccgcggccgg tcggcctggg cacattccag
    10981 gagacgccct ggggcggcgt gttcgccgat gcgttcgtcg acaccgtctc cggcggcacg
    11041 ctgctggagg cgtcgtacgc ggccgaagtg cccgtcggcc cggtgacgct ctatccgcag
    11101 gccggcctcg cgcgacgcag cgcgcgctac gacgcgcatc tgtacgacgt gcacgccgcg
    11161 tcgaccacgc ccgtgctgca gctgtcgggc gagctgccgt tcgccgacac ctgggtcgcg
    11221 ctggcccacg tgcagtggga gccgttcgac gccgccgtgc gcgccagccc gcgcgtgtcc
    11281 gcgcgctcgc gcacgtcggc cctggtcgcg ctcgcgtggc gcttcaagtg atggacgacg
    11341 tcatcctcta tgcgctgccg gtgttcggcg tgctgatggc cgccgagttc gcctggggac
    11401 tcgcgcgtgg ccgcaacaac tacggcgtgg ccgacacgct ggccagcctc agccaggggc
    11461 tgctgagcca ggtggtggcg gtgtgcacgc agttcgtgca ggtcgggctg tacgccctgg
    11521 cgtacccgct tctgacgctc acgccgcatt cgccgctgtg gcagcgctgg cccggcgtcc
    11581 tgctcgcggt gttgctgtac gacttctgcg actactggct gcatcgcgtc tcgcacgaaa
    11641 gcgcgctgtt ctgggccgcg cacgtcgtgc accaccagag ccggcgcttc aacctgtcga
    11701 ccgcgctgcg ccaggagagc gcctacctgc tgaccggatg gccgttcttc ctgccgctgg
    11761 ccatcgtggg cgtgcccccg gcgctgttcg gcgtggcggc cctcgtcgtg ctgttctacc
    11821 agttctggat ccacaccgag cacgtcggaa ggctcggctg gctcgatcgc tggctgtcga
    11881 cgccgtccaa ccatcgcgtc caccatgcca ccaatgcgcg ctacctcgat cgcaactacg
    11941 gcgcgatcct ggtcgtgtgg gaccggctgt tcggcagctt cgagccggag tccgagccct
    12001 gcgtctacgg cacgacggtg ccgctggccg ggtgggcgcc gctgcatgcg gtcagcgtca
    12061 actacgtcga gctgtggcgt cgcttcctgc gcgtgcacgg ctggcgcgac aggctgggcg
    12121 tcctgttccg ggcgccgggc tggcgcgcgg cgtcggagcc cgtgccgggg ccggtcgcga
    12181 tccccgccga cgtgccgctc ggccgcggtc gagcggtggc ggcgtgcgcg ctgttcgtgc
    12241 tcgcggtcgc gtgcaccggc ggcctcctgt ggcgcgccga cacgctcggc ccggcggcgc
    12301 tcgcgggcgt cgccgtcgcg ctcgtggccg ccctgtgggc gatcggcgcg ctgctcgacc
    12361 gtcgcctcgc gccgtgggcc gccggcctcg cgggtgtggc atcggccacg gcgacgctga
    12421 tcttgttgcg cgcggcctga ggacttggcg cgtgctggcg cccgagccgg agtacgctag
    12481 ggcgtcacca gcatcgggtt cgacatgatc ggtctgcact tcggagttcg cgtgcgggca
    12541 gcgctggcgc tgctgggcgc gctggcctgc gccgtcccgt cgccggctgc cgacgctccc
    12601 gcgccgttgc acctgcgcat cgtcggcggc ctgggcagcc tgaaccagtt cacgcgccat
    12661 gaacgaccgt tctgggagac ggacctcgcg cggctgagcc agggccgcta caccgccgag
    12721 atcgtgccgt tcgaccgcgc cggcatccgc agccaggagg tgctgacgct ggtgcagctg
    12781 ggtgcggtgc cgttcgccgc gctgccggtg tccggcggca tcaccaagga tcccgagctg
    12841 accgcgcccg acctggccgg cctgaacccc gacatggcct cgatgcgccg ctcggtcgcc
    12901 gcgttccggc cctacctcga gaccatgatg cgcgaacgct acggcatcga ggtgctggcg
    12961 ctgtacgtct acccggccca ggtcgtctat tgcaagaagc cgttctcggg catcaagggc
    13021 ctggccggcc tgcgcgtgcg cacctccggg ccggcgcagt cggactgggt ggaggccctg
    13081 ggcggcaagc ccgtcaccac cggcttcggc gacatcctgc ccttcctaga gcgcgacgag
    13141 ctcgactgcg cgatcaccgg caccatgtcg ggcaacacga tcgggctgga caaggccacc
    13201 acgcacatcc atccgatggc catcagctgg ggcctgtcgc tgttcgtggc caacggcgcg
    13261 gcctggaatg cgctgccggc cgacctgcag gcgctgatcc ggcgcgagct gccgcgcgtg
    13321 gagcacgaca tctgggacga gtccgagcgc gagaccgccg acggcctggc ctgcgacatc
    13381 ggcgctgcgt cgtgcaaggc gggccggcgg ggccacatgg ccgaggtgcc gtcctcgccc
    13441 gccgacgagc gcctgcgccg cgagatcttc gcctccacgg tgctgccgcg ctggctgcag
    13501 cgctgcgggc cgcaatgcgc ggcggtgtgg aaccgcacga tcgcgcacgt cgcggggatc
    13561 gaggcccgat gacgccttcg ctgagacgct cgagcgcgat cgtctggggc gcgacctgcg
    13621 cgctgctgct ggcgctggtg gtgaccgccg gctggctggt ggccgcaggc cggcgcgacg
    13681 cgatcgcccg cgccgaggag gccgtcacgc agacggcggt cgcgtcggag tccgagctca
    13741 atcgcgcgct gctgtcgctg gacctgcagc tcaccagcct ggccggcgtc atcgcgcccg
    13801 cctggcgcgc gccgggcgag ctcgacgccg aggccgcgca ccgcgcgatg gccgcgttca
    13861 aggatcgcca gctgctgttc accgatgccg cgctgatcga cgaccagggc cacacgctgg
    13921 ccgccagcct gccctcgagc gaccgcaacg gcatgcacct gcccgacggc tttgccgcca
    13981 ccctgctcgc gcagcgggtg ccgcagctga ccatcagcga ccccgcgatc agccccgaca
    14041 ccacggagcc ctccgtctac atgggccgcg tcgtcgcgct gcccgacggg cggcgcgcgc
    14101 tggcgctggc cgaggtgccg gtgaccgtgc tcgcggcgat catcgcgcag gccggcggca
    14161 gcccggcgct ggccgtcacg ctggagcgcg gggacggcca gctgctggcg acggtgcctt
    14221 cgggcgcggg cctgcgcccg cgcgaggcgg ccgagccggt gcccgacgaa gcgctgcggg
    14281 gccgcccggt cgtcgcgccc tcgcggctga ccggcgtgcc ggcgatcgtg gcggcgcggg
    14341 cgacgctgta ccgctcgctg cgggtgaccg cgagcctgcc gctggaggac gcgctgcagg
    14401 cctggcgcaa caaccgcaac atcatcctga ctgcgtcggt ggcgctgatc gcgctgacgc
    14461 tgctgggcgg cgcgatgtcg cagtggcagt tcaaccggct cgtcgacgcg cgcagtgccg
    14521 ctgcgcggtc cgtggaggcg ctggacaagg cgctggtcgt gctgcccgac gggttcctgc
    14581 tgtgcgatcc cgacgaccgc gtcgtgcgct ggaacgagcg cttcctcgag atgtttccgt
    14641 ggctgcgcgg cacgatccgc gtcggcatga cgcacgccca gctcgtcgac gccacgcgca
    14701 aggccaccga gttcgacgcc accgaggagg cgggcgagca gtggctggac gagcgggtgc
    14761 gcacgcgccg gctgggcgag gccgagtggg aacgcgagct cgccaacggc atggtggtgc
    14821 acatcaccga ccgccgcacg cccgacggcg gcatggtcag cctcttccac gacgtcaccg
    14881 ccgccgagcg ccggctcgcg caggccaagg agaacgcgga ggccgccaac caggccaagt
    14941 cgcagttcct ggccaccatg agtcacgaga ttcgcacccc gctgaacgcc gtgctgggcc
    15001 tgaacgagct gatgctgcac tcgccgctcg actcgcagca gcgccgccat gccgagctga
    15061 tcggcagctc cggccggctg ctgctggcgc tgatcaacga catcctggac gtctcgcgca
    15121 tcgaggccgg ccagatgcag ctcacgtcgg cgccgttcag cgtgcggccg gcggccgagg
    15181 gcgtggtggc gctgatgcgc gagcgcgccg tgtccaaggg cctcgcgctg cggctggacg
    15241 tttcgccgca cgacgacgcg ctgatcgagg gcgacgtgat ccgcatccag cagatcctgt
    15301 tcaacctggt gggcaacgcc gtcaagttca ccgaccgggg cgtcgtgcag gtgaacgtga
    15361 cgctggagcc ggccgcgggg cgcgacgtca ctctgcgcct ggaggtcagc gacaccggca
    15421 tcggcattcc cgagagcgcg atggccacgc tgttcgaccg cttcacgcag gccgactcca
    15481 ccaccatgcg gcgttacggc gggagcgggc tggggctggc gatcacgcgc gagatcgtcc
    15541 agctgatggg cggcagcatc gccaccgcca gcacgcccgg ggtgggcagc cgcttcacgg
    15601 tcacgatccc gacccggcgc gtcgagcgcg gcgcgccggc gcccgacgcg gcgcggtccg
    15661 acgaggcccg gcccgcgcgc ccggcgctgc ggctgctcgt ggccgaggac aacgacgtca
    15721 accagatcct gatcaacgcg ctgctcacgc gcatgggcca tcgcgtgcac ctggtggcca
    15781 acggccgact ggcggtcgag gccgtgcgcc gcggcgacta cgacctggtg ctgatggacc
    15841 tgcagatgcc cgagatggac ggcatggagg ccacgcaggc gatccgcgcg ctgggcggcg
    15901 ccttcgcgtc gctgcccgtc atcgcgatga ccgccaacgc gttcgacgag gaccgccagg
    15961 cctgcctggc cgccggcatg gacgactacg tcgccaagcc gatcgacgtc gcgcacctgg
    16021 ccgaggccat cgcgcgctgc accgcgaccg tcggctgaac gtacgcgtcg cgaccgggca
    16081 cgggccttgc gtgcgaaact gtccgcctgg atgatgccgg ccggcgaccg cacccgcgtc
    16141 gacccgtgcc ggccgcctcc ggacgccgtg aacccgaccc gcacgatgct tcgcctcgaa
    16201 ctcaccgaca tcagcaagca gtaccccgcc gtcaaggcca acgaccgcgt ccgcctgcgc
    16261 gtcgaggcgg gcgagatcca cgccgtgctg ggcgagaacg gcgccggcaa gtccacgctg
    16321 atgaagatca tctacggcgc gacgcggccc gatgaaggcg agatgcgctg ggacggccgc
    16381 gcggtcgaga tccgcaatcc gcaggaggcg cgcgcgctgg gcatcgcgat ggtgttccag
    16441 cacttcagcc tgttcgacac gctcaccgcg gccgagaacg tctggctggg gctggacaag
    16501 gcgtggtcgc tggccgacgt cacgaagcgc atcaccgagg tggcgcacga gtacggcctg
    16561 gacgtcgacc cgctgcgccc ggtgcacacc ctgtcggtgg gcgagcgcca gcgcgtcgag
    16621 atcgtgcgcg cgctgctcac caagccgcgg cttctgatcc tggacgaacc cacttcggtg
    16681 ctcacgcccc aggccgtgca ggccctgttc gtcacgctgc gcaagctcgc cggcgagggg
    16741 tgcagcatcc tgtacatcag ccacaagctc gacgagatcc gcgcgctgtg ccacaagtgc
    16801 acggtgatgc ggggtggccg cgtcacgggc gaggtcgacc cgcgccagga gagcaacgcc
    16861 ggcctgtcgc gcctgatgat cggcgccgag ccgccgcagc tcgaacatcg ccaggccacg
    16921 cccggcgagc cggtgctggc cgtgcgcggg ctcacgctgg cgcgcaccgg cgcgttcggc
    16981 gcggacctcg cggacgtcac cttcgaggtg cgccgcggcg agatcctggg catcgcgggc
    17041 gtctcgggca acggccagca ggagctgatg gcggcgctgt ccggcg
//
LOCUS       DATCRO010000620         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_322242, whole genome shotgun sequence.
ACCESSION   DATCRO010000620 DATCRO010000000
VERSION     DATCRO010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322242"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VIN75_16875"
     CDS             <1..416
                     /locus_tag="VIN75_16875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8975895.1"
                     /translation="KRVLLMLAFSLAGFALLGWLATQWGFYVGLGVTPNVMASNNALA
                     LLLFTMGLPPFMFFVSPLFASLSRRDEYQADAYARSNANAADLVRALLKLYEDNASTL
                     TPDPVYVRFYYSHPPASSRIAALGLADVPLAGVPA"
     gene            413..1348
                     /gene="rsgA"
                     /locus_tag="VIN75_16880"
     CDS             413..1348
                     /gene="rsgA"
                     /locus_tag="VIN75_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8975896.1"
                     /translation="MSARSSRGAPPPSELALARVVGVHGRHSVIETPDGRRLLAHGRG
                     KKSETVVGDHVRWQVSGDEGVIESVEPRTSLLKRQDEWRSKNFAANIDLLLVMVATDP
                     PYSESQLARALIAAEDGHIDVKIVLNKIDLPEAAAARERLAPYRRMGYDVLEVAVKAD
                     PAGTKDRLQPLLAGKTTLVLGPSGMGKSTLVNLLVPHADAQVGEISKALKSGKHTTTT
                     TTWYWLDDARHSALIDSPGFQEFGIHHIEAPRLASLMRDYAMTFGDCKFANCTHRHEP
                     SCGVRAAIARGDIAPTRERIYEELMDELERAASRH"
     gene            1344..1877
                     /locus_tag="VIN75_16885"
     CDS             1344..1877
                     /locus_tag="VIN75_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8975897.1"
                     /translation="MEDEPDGAPSHTHAGRCRPASSPAAPSNQRRDAMTIAWISFTPW
                     LSLAGGLLIGAAAAMLILFNGRIAGISGIVGGLLQPVRGDIDWRVAFVLGLLAAPLAW
                     LAFAAAPRLHVAASLPVLVAAGLLVGVGTRVGSGCTSGHGVCGISRGSLRSVAATATF
                     MAAGFAVVFVVRHLLGG"
     gene            1879..2316
                     /locus_tag="VIN75_16890"
     CDS             1879..2316
                     /locus_tag="VIN75_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY8975898.1"
                     /translation="MRIVTAFIAGLVFGLGLLLSGMADPAKVLGFLDLAGAWDPSLVF
                     VMGGAVGAATVAFAAARRRKTSVLGEAMQLPTKSGLDRRLVAGALLFGTGWGLAGFCP
                     GPAIVALGVGEPKAVVFVAAMLAGMVLFAFFERRRANALPAAR"
     gene            complement(2338..2862)
                     /locus_tag="VIN75_16895"
     CDS             complement(2338..2862)
                     /locus_tag="VIN75_16895"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HEY8975899.1"
                     /translation="MPTTAKPNAVSRADLRDRLLILLVGLGQVALPCIVVTQPLLAFA
                     SRPQPDACTVLGVVVMLAGLRLFWRSHADLGESWSVTLELNADHRLVTEGVYRHVRHP
                     MYASFLVLGVGQALLVANWIAGLSGPAAVALLMAVRLPREEAMMIERFGDQYRDYSRR
                     VGAILPRRRQAGAA"
     gene            2852..>3118
                     /locus_tag="VIN75_16900"
     CDS             2852..>3118
                     /locus_tag="VIN75_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiB"
                     /protein_id="HEY8975900.1"
                     /translation="MVGNFQDAIDGWKRDAGLWKHANEGIILAAAAGAVGVQLGGATP
                     PGVTPDRSKTFTSGEASLASESAGSTTQGMPPQLGHLGIVVGLVW"
BASE COUNT          427 a         1137 c         1105 g          449 t
ORIGIN      
        1 ccaagcgggt gctgctgatg ctcgccttca gcctcgcggg attcgcgctg ctgggctggc
       61 tggccacgca atggggcttc tacgtcggcc tgggcgtcac gcccaacgtg atggccagca
      121 acaacgcgct ggcgctgctg ctgttcacga tgggcctgcc gccgttcatg ttcttcgtct
      181 ccccgctgtt cgccagcctg tcgcgccgcg acgagtacca ggcggacgcc tacgcgcgca
      241 gcaacgcgaa cgccgccgac ctggtgcgcg cgctgctcaa gctctacgag gacaacgcgt
      301 cgacgctgac gcccgacccg gtgtacgtgc gcttctacta ctcgcacccg ccggcgtcgt
      361 cgcgcatcgc cgcgctgggc ctggccgacg tgccgctcgc cggagtgccg gcttgagcgc
      421 acgctcgtcg cgcggcgcgc cgccgccttc cgaactggcg ctggcacggg tcgtgggcgt
      481 gcacggccgc cattccgtga tcgagacgcc ggacggccgg cgcctgctcg cgcacggccg
      541 cggcaagaag agcgagacgg tggtgggcga ccacgtgcgc tggcaggtgt cgggcgacga
      601 gggcgtgatc gagtcggtcg agccgcgcac cagcctgctc aagcgccagg atgaatggcg
      661 cagcaagaac ttcgctgcca acatcgacct gctgctggtg atggtggcca ccgacccgcc
      721 ctacagcgaa tcgcagctgg cgcgcgcgct catcgccgcc gaggacgggc acatcgacgt
      781 gaagatcgtg ctcaacaaga tcgacctgcc cgaggccgcc gccgcgcgcg agcggctcgc
      841 gccatatcgc cggatgggct acgacgtgct ggaggtggcg gtcaaggccg acccggccgg
      901 caccaaggac cgcctgcagc cgctgctggc cggcaagacc acgctcgtgc tgggccccag
      961 cggcatgggc aagagcacgc tggtgaacct gctggtgccg cacgccgatg cgcaggtggg
     1021 cgagatctcc aaggcgctca agtcgggcaa gcacaccacc accaccacca cgtggtactg
     1081 gctggacgac gcccgccaca gcgcgctgat cgactcgccc ggcttccagg agttcggcat
     1141 ccaccacatc gaggcgccgc gcctggcctc gctgatgcgc gactacgcga tgaccttcgg
     1201 cgactgcaaa ttcgccaact gcacgcaccg ccacgaacca tcctgcggcg tgcgcgccgc
     1261 catcgcccgc ggcgacatcg cgcccacgcg cgaacgcatc tacgaggagc tgatggacga
     1321 actcgagcgc gccgcgtcgc ggcattgagg acgagccgga tggggcgccc tctcacacgc
     1381 acgccggccg atgccgtccc gcaagctcgc ccgcggcccc atcgaaccag cgtcgcgacg
     1441 ccatgaccat cgcctggatc tccttcaccc cgtggctgtc gctggccggc ggcctgctga
     1501 tcggcgccgc cgcggcgatg ctgatcctgt tcaacggccg catcgcaggc atcagcggca
     1561 tcgtcggcgg cctgctgcag ccggtgcgcg gcgacatcga ctggcgcgtg gccttcgtgc
     1621 tggggttgct ggccgcgccg ctggcgtggc tcgcgttcgc cgcggcgccg cggctgcatg
     1681 tggcggcgtc gctgccggtg ctggtcgccg ccggcctgct ggtgggcgtg gggacgcgcg
     1741 tcggctcggg ctgcaccagc ggtcacggcg tgtgcggcat ctcgcgcggc tcgctgcgct
     1801 cggtcgcggc caccgccacg ttcatggcgg ccggcttcgc cgtcgtgttc gtcgtgcgcc
     1861 acctgctcgg aggctgacgt gcgcatcgtc accgctttca tcgccggcct cgtcttcgga
     1921 ctcggcctgc tgctgtccgg catggccgat ccggccaagg tgctgggctt cctcgacctc
     1981 gccggcgcgt gggatccgtc cctggtgttc gtgatgggcg gcgccgtggg cgcggccacc
     2041 gtggccttcg cggcggcgcg ccgtcgcaag acatctgtgc tcggcgaagc gatgcagcta
     2101 ccgaccaaga gcggcctcga ccgccgcctc gtcgcgggcg cgttgctgtt cggcaccggc
     2161 tggggcctgg ccggcttctg ccccggtccg gccatcgtcg cgctgggggt cggcgaaccc
     2221 aaggccgtcg tgttcgtcgc cgcgatgctg gcggggatgg tgctgtttgc gttcttcgaa
     2281 cgccgccgag ccaatgcatt gccggccgcg cgatgagcgc gcgccggcgg ccagcgctca
     2341 agcggccccg gcctgccgac gtcgcggcaa gatcgcgccg acgcgccggg aatagtcgcg
     2401 gtactggtcg ccgaagcgtt cgatcatcat cgcctcctcg cgcggcagtc gcacggccat
     2461 cagcaacgcc accgcggcgg gaccggacag gcccgcgatc cagttggcga ccagcagcgc
     2521 ctgccccacg cccagcacga ggaacgacgc gtacatcgga tgccgcacat ggcggtagac
     2581 gccttcggtg acaaggcgat ggtcggcgtt gagctccagc gtcaccgacc agctctcgcc
     2641 aaggtccgcg tgcgagcgcc agaacagccg caggccggcc agcatgacga cgacgccgag
     2701 caccgtgcag gcatcgggct gcgggcgcga cgcgaatgcc agcagcggct gcgtcaccac
     2761 gatgcacggc agcgcgacct ggccgaggcc gaccagcagg atcagcaggc ggtcgcgcag
     2821 gtccgcgcgg ctgaccgcgt tcggcttcgc cgtggtcggc aacttccagg acgccatcga
     2881 cggctggaag cgcgacgccg ggctgtggaa gcacgccaac gagggcatca tcctggccgc
     2941 cgcggccggc gcggtcggcg tgcagctggg cggggccacg ccgccgggcg tgacgccgga
     3001 ccgttccaag accttcacca gcggcgaggc gtcgctggcg tcggagtcgg ccggcagcac
     3061 cacgcagggc atgccgccgc agctggggca cctgggcatc gtcgtcggcc tcgtgtgg
//
LOCUS       DATCRO010000621        19119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_929310, whole genome shotgun sequence.
ACCESSION   DATCRO010000621 DATCRO010000000
VERSION     DATCRO010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19119
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929310"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VIN75_16905"
     CDS             <1..138
                     /locus_tag="VIN75_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8975901.1"
                     /translation="GVEANRVYTEGKGKKQPVADNKTAEGRAKNRRVEIEVVGTRATG
                     K"
     gene            273..983
                     /gene="ubiG"
                     /locus_tag="VIN75_16910"
     CDS             273..983
                     /gene="ubiG"
                     /locus_tag="VIN75_16910"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY8975902.1"
                     /translation="MTNADPQELEKFGELAHKWWDTEGDFKPLHRINPLRLDWIAQRT
                     PLHGRSVLDVGCGGGILAESMAQRGASVLGIDLSVKPLKVAKLHAMESGVGNLEYREV
                     AVEALAAERPASFDAVTCMEMLEHVPDPASVVASCARLVKPGGMVFFSTLNRNAKAFL
                     FAIVGAEHLLKLLPKGTHEYAKFIRPSELAGFAREAGLETLELAGLEYNPLTQRYWLS
                     GDTSVNYMVACRRPAASS"
     gene            980..1651
                     /gene="gph"
                     /locus_tag="VIN75_16915"
     CDS             980..1651
                     /gene="gph"
                     /locus_tag="VIN75_16915"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549795.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY8975903.1"
                     /translation="MSAPLRGVLFDLDGTLIDSAPDLAGAANRLRADHGLEPLALESL
                     RPMVGSGARGMVGVAFGVAPGDERFEGLRDAFLAHYEAGLLELTRPFDGVLQMLSALE
                     AGGIAWGIVTNKATRFTLPIVKGLGLAQRAGVVVCGDTTPHSKPHPEPLLHAARTLGL
                     APEHVAYVGDDLRDAQAARAAGMPMLAATWGYLGLGEPAHSWGAERLVDAPSQVLDWL
                     RARAG"
     gene            1903..2532
                     /locus_tag="VIN75_16920"
     CDS             1903..2532
                     /locus_tag="VIN75_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8975904.1"
                     /translation="MKKILAMAALAVASVSASAATNLFVDGSFESITQPAGTWSTYHV
                     VPGWTVTQAGSFLPSPHGLEIRNDVAGSAQDGNNFIELDGYENDRITQSFATTIGKTY
                     EVSFWYQNRAGVAAASEGFEAVVLSGGVTSASLVGNAPSDGWLQETMVFTAGSNFTTF
                     TIAATGTSDSLGTSFDDFTALAVPEPATMGLFAAGLAIIGLSARRRRRN"
     gene            2675..3047
                     /gene="ssrA"
                     /locus_tag="VIN75_16925"
     tmRNA           2675..3047
                     /gene="ssrA"
                     /locus_tag="VIN75_16925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3559..4035)
                     /locus_tag="VIN75_16930"
     CDS             complement(3559..4035)
                     /locus_tag="VIN75_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8975905.1"
                     /translation="MTTSNLYQPMNMIPLHAAAPNDANALLARAAAPARDRRSHARRR
                     GDFLFGRREVSAARREVLVDGKPRTLQPRPFDVLVYLIEHRERVVSTDELLDHIWKDE
                     FVQPGSLAAAVMRIRKALLDVGADAGAIIRTHQGIGYRFVAELDGDDPAGTAEHDA"
     gene            complement(4051..4449)
                     /locus_tag="VIN75_16935"
     CDS             complement(4051..4449)
                     /locus_tag="VIN75_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8975906.1"
                     /translation="MFVVPVHLPPGAPPAPGDVDETLALVRHEPVIRFGRCELRTARR
                     ELFVDGVRRPLQPRPFDLLVYLVENGDRVVTSEELLSQVWGDASVQRWSLPAAVLRLR
                     RALGPNHGDVIRTYQRVGYRFVAPAIPASM"
     gene            complement(4573..5397)
                     /locus_tag="VIN75_16940"
     CDS             complement(4573..5397)
                     /locus_tag="VIN75_16940"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8975907.1"
                     /translation="MQKHGGGSGCMPAGTGAIPRPTRAIAGQRPVFARQFTLSRAGAP
                     RSEPGHASRGDVSYIESSSSTRACRMSIEVDRHGRRTAWLTARLCTVLGVSAIESRDW
                     AVAALTHDIGKLDLPPALLAKPGALDADERCLVERHCIAGAKRLMARARPDGDETTTA
                     AIAVALSHHEWWNGQGYPFGLAGTAIPRCARIVAMADVLDALTSVRPYKPAWPLQAAL
                     DHVLARRGTQFDPECVDAMEIAARTLPADWQGTAQAWGLQMTLELGRPARVLLDAT"
     gene            complement(5426..6316)
                     /locus_tag="VIN75_16945"
     CDS             complement(5426..6316)
                     /locus_tag="VIN75_16945"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY8975908.1"
                     /translation="MGLRDRKAAGDGQIVNPGRPCVTGRNPRVRPSTATVTSVTPRPR
                     SHSAPSPGHVAIMTCVVPRTTILCRSGSMAIRRQGSGCMGAMTTDFQGSSSAGARGRA
                     SGFSFIEMMLTLSVGGIVLALAVPSFDRAIADIHTRSTAQQLAGALRLARASAVTRNR
                     PAAFMLTDAAPQTDAVAAVHAGNWLVKFLPSTPRPQAARGADVILATTASARDRVTLT
                     GPAQVCFDAQGLQMSTPDTPDGTRIACAQPGADLGGVTSYLVARSDATRQYKVRVWRT
                     GRVDICDMARTHGKDLDGCA"
     gene            6327..7469
                     /locus_tag="VIN75_16950"
     CDS             6327..7469
                     /locus_tag="VIN75_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8975909.1"
                     /translation="MPLSSQPTTAPPVDVLLIEDNAADARFFGLLLESSPAAGNFRLR
                     HAATLAAGVAMLGARRPDVVVLDLGLGESEGLATVQHLVASGVRLPALVVLSGLDDED
                     VAVEALQAGAQDYLIKGQIDGRQLARAIRYAIGRHQAEHAIAERTAELERSRELVRSL
                     GARNARLLEEERRRVARELHDEMGQQLAALRMEIAVLRQGRVAAAQASSDAALGMLLG
                     RVDGLVVSVRRIVAELRPPALDGGLAAALDWLVSDFERQTQLDCEVDVDACASLLSAD
                     AAAMVFRIAQESLNNARRHAVASKVSLRLQRTDGGCELRVSDDGGGFDPSERTAGFGI
                     LGMEERARALGGSLDIDSRPGQGTEVRLRLPLSEARGVLPAARPDS"
     gene            complement(7497..10532)
                     /locus_tag="VIN75_16955"
     CDS             complement(7497..10532)
                     /locus_tag="VIN75_16955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY8975910.1"
                     /translation="MGWSTERLRAIVVAVTLAATAGVGHAQIADMEPYQESAGLTSMA
                     VECLAQGPDGTMWIGTDNGLFVFDGFRIRRETLPKGAAATITDLQADRFNRLWIATDT
                     GLYLRREDAGVAHWSAIVDPQGVGLAAWGKQRVAVDEHGAVFAMDRRSRIWTITVPPR
                     LPDKVVASRMTMPAYEPYAGAYDADGGPLRAVGPALWFGCGHGLCRWLDGQLRAWGPD
                     QGLPADTWASLLAARDGSVWARGDRHLARLAPDADRFETVDAPSATLWPGTIAIAEDL
                     EGRILTAVEDGVARWDGRSWRRWTPHEGIPETAVRALLVDADGELWTGSAGRGVHLWT
                     GYGRVEHWTSVSGLPSPVVWSFARDGAGQMRVATSRGLAALDPRTRRFVAVEPLFAPT
                     PGGSLATDDAGTVWWVDAGHVMSLAPGMRRARVAFRDPAIDVVLQGAHSIVLSGTSSA
                     ERLVPAPAGPVRQPLPAHMPDPSSLVAVVSDGQREWFLVGNGAWRIDRGGWEPLRDER
                     GNPIEVMGAAAFAGPSEFWAHDSKGLATYRVVDGTARLKQRIDPSQLGTADIEFLGAD
                     SERRVWVGTDRGVFVHTDARWMQLDRDNGLLWNDVDTGAIHFDADGGVWIGSSKGVTH
                     VLPGRLAADAPRLRVAEVRFGDRPGARAMPPRVDWDDRRLRLTLQTAQIARGRSMRLE
                     YRLNDAQTWEGFVGNVLQLESLEAGDYRLQVRAAANAPLLQAGIPLELAFTVAPPWWR
                     STPARITYVLGLAAAWFLSMQLLRLRARAMRTRLEQAIADRTAELERSRELVRSLGVH
                     NTRLLEEERKRVARELHDEMGQQLAALRMEISVLRRRTSDHDASSTEAALGMLLVRVE
                     DLVTGMRSVVAELRPPALDGGLAAALDWLVAAFERHADLPCVAEIDECATLLSPDAAV
                     MVFRIAQESLNNVRRHARAGRVWLRLARAGQGCELTITDDGVGFDPAARTSGYGILGM
                     EERAIALGGTLAITSATGQGCTVRLRLPLPESGPASGSR"
     gene            complement(10516..11175)
                     /locus_tag="VIN75_16960"
     CDS             complement(10516..11175)
                     /locus_tag="VIN75_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975911.1"
                     /translation="MSTSAAAAVQKHRILVADDHALVREGIGKVLGLDERLVVAGEAV
                     SAAEVLARLKRGGIDLVLLDLLMPGATDVDLIKRVVAAHPRLPVLVLTMHADPHIARR
                     ALEAGALGYLTKDASPELLVDAIHHVLAGRSYVDPSLSGALIRGEPASPREQLEQLST
                     RERQVLVALVTGRSVMDIADELQLAPNTVSTYKGRLMEKLGQTSLSDLVRYALRHGLV
                     D"
     gene            complement(11243..11887)
                     /locus_tag="VIN75_16965"
     CDS             complement(11243..11887)
                     /locus_tag="VIN75_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975912.1"
                     /translation="MSKPETRKSETEARRLWLRLSEYLQRRIRLRRSGLAWRLALEPA
                     GAPRQAPPAPRPRPVRRPQLPPMPGAHASAARHELRVLLDHVAGARRVWPSLALLERV
                     LGSDSADNIHRVEAAVLRHAARALDQLGDDLFGPGLVVLRRRVELVLRRKHGDRPSHW
                     VRMPPPVGPEQAAAFGDSLTDYIEMDRVPLAATPARAGRRSAHPARGEKERSVV"
     gene            12027..12518
                     /locus_tag="VIN75_16970"
     CDS             12027..12518
                     /locus_tag="VIN75_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY8975913.1"
                     /translation="MSSNPGKCRTKRHAGFTLIEVMIAVAIVGILTAIALPSYRNYVI
                     RGKLVAGTNALANMRTQMEQYYQDNRTYATVSAPAIVTPCVANAVVASNGTPFTVGCS
                     AASDVPTATTYTLRATGTGTVAGAVYTIDQANNMATVSFPTSWGSVPANNACWIMRKG
                     DSC"
     gene            12533..13114
                     /locus_tag="VIN75_16975"
     CDS             12533..13114
                     /locus_tag="VIN75_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8975914.1"
                     /translation="MPRGFSLIELMVTVALIAILLVVAIPAYGTWTADAHVRAAAESL
                     TNAIRLTQATAVAKGRTSLFALTTDAQPSVNSAPAAYAPNWFATLNVLGGSDETKASL
                     GLILKSTEGTQHRVTISGPALVCFNALGRQTSLAAAANSLSTACTAGDPAVFQVSRDA
                     GASRYFKVLVYAGGRVRMCDAAKAISSADPDGC"
     gene            13117..13536
                     /locus_tag="VIN75_16980"
     CDS             13117..13536
                     /locus_tag="VIN75_16980"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8975915.1"
                     /translation="MQQRHRMPGLRRSTRGFTLIEVLIAILVFSFGLLGAAAMQAVAV
                     QAATQNGNRSRAALLANEMVSTLWATQSSTPAPADLAAWTSKIATPSASGLPNGSGTV
                     TACTGVANCAVITVTWKAPSAKTAAASNRYTTTVVIQ"
     gene            13533..14735
                     /locus_tag="VIN75_16985"
     CDS             13533..14735
                     /locus_tag="VIN75_16985"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY8975916.1"
                     /translation="MKRSPSPQAGVTLIEMMVALVIGMVLSIAVMMVLSTFEGRRRTL
                     GSTSDLDQAGALAMFQLDRWMRSAGTGLGQGSSFAYGCTLFAARSGHQVLPLTGPLPA
                     PFASVDPGTSGVFRLAPVLILPGQTMPNISGKASDVLVVMSAGNDASQVPSPLSAPPA
                     ADHLAIDNLTEFSPGDLLLVADHHQSPGGGAANCMVSQVSSSLTTSGTGTTLPLGGSD
                     STYASASIGSAAITSLTDGPVAFDLGAPTASGTSQPPSFQLIGVGDNDTLYGYDLLQV
                     SGSSAQAQAEGVFEMHAVYGIHSSANADNKVDQWVDAGATSAYSVAALSDGSVGATTR
                     LKNILAVRVALILRTALPEKDAVTPHALTMFTDLPGLAITRTLTSAEQHFRYRVVETT
                     VPIRNNGF"
     gene            14735..15400
                     /locus_tag="VIN75_16990"
     CDS             14735..15400
                     /locus_tag="VIN75_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975917.1"
                     /translation="MSAPTLSHRARRLRGVRTLRPQRGIVMVFTLIALVLMLIAVAAV
                     VRSVDTSSGVVGNLAFRRDLTNRAELAIAQAKAALNAGGAVYAESARRADLAAAHYSA
                     SRLASPPGGIGVPAVLVSNSAYDSAGYSCMNGAGVTLPNCATPGADGIVIRWVIDRQC
                     VAGTSAFGTSACAYLNNVTPPGGTGHSLTRKPRGGGRSVFRISVRVTGPRNTEAYIQT
                     TAG"
     gene            15405..>19119
                     /locus_tag="VIN75_16995"
     CDS             15405..>19119
                     /locus_tag="VIN75_16995"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY8975918.1"
                     /translation="MPSTLLAPVRSRLLSPIAFVLALLAASAAVAQGVDLADKPLFST
                     TSVPGNLLLSLSVEYPTANTSAYFSTTPYASGTLYLGYFDGAKCYRYLYNSASPASSY
                     FQSYGAASASHQCTSSSSLPLWSGNYLNYAAMQSLDIFRWVMTGGNRLVDTAGPNGMT
                     ILQKATHSGQGAAGDAPNKSIPYADQAGATPFNWGNGVKSRIWGAGIKLWLTGSGDLN
                     PTNITAVDYQDSLMANGSPDGATVYALYMNVRVCDATVSLEANCVAYGSQSKPEGLMQ
                     QYSGSLRYSAFGYLNDSSPWRDGGVMRARMAYIGPKIPQPGATATINPNPEWDATTGV
                     MIVNPDPADAAATNTSTNNRTAVSQSGVLNYLNKFGYASGTYKTYDPVSELYYASLRY
                     LKGQTNVAAYSDMSGASAATASTWTDGFPVVTDWSTDPIIYACQKNFILGIGDVNTHR
                     DGNLPGSPASDLEPAQPPEVRADASGVSPVDVFASTNLVGSMEGQSNLSWRWATGDEG
                     RFYIAGLAYDAHVPIAGKGVRTFTDANTNVTTVSTASTYWLDVLEYQAYVNNNQYYYA
                     TKYGGFTVPTGYAYGGALPTDSWYNSTNTLGSNKQPDNYFTASNATLMRSGLTNAFAK
                     IVSENNAANGTALSLPTPNVSASGSMSYSATYDPTSWTGQVIGSTVVFNADGTSTLTQ
                     RWDARALLGDPNVTAATRKIVTCCALNATSPGLPFTLTSLRAASLDSRTYYASFANVP
                     GVASSSQSIANYIAYLRGDTSKEVANGGVYRTRKFRLGDIVDAKPVTVGPPNSGFYDT
                     YNPGYAAYRTQYLNRRNVVYAGANDGMLHAFDGTVDNTNPVSGSELFAYIPSFVYGNA
                     STAAATGLASLGNPAFTHHYLVDATPGEFDIDLNRTSGAAGDPKWRTLLIGGLGKGGK
                     GYYALDVTDPTTWSDEATVASKVMWEFTDSRMGYTYGTPTVAKTKKYGWVVIFTSGYS
                     NADGRGWFFIVNPSSGALLEAVATPAGTGSTSSPLNMGQATTYIENYTDMTADAVYAG
                     DLQGNVWRLDVSATDVTLPYPTPTRIARLTDDRGNAQPITTRPLIEIAPNSTTRYVMV
                     GTGKLLADSDIASGAQQSFYAFVDGLATSGAFYGAPGQPLPTGVSFPITRSQLNADTS
                     VLDGIGSSPSSAMGWYLDLSVSGGIAERVNVDPIASQGVVAFIGNQPNGSACSPSGNG
                     TLHTLSIAAGQSLLLNTDLVTPVASIAITGIGTD"
BASE COUNT         2949 a         6792 c         6547 g         2831 t
ORIGIN      
        1 ggcgtggaag ccaaccgcgt ctacaccgaa ggcaagggca agaagcagcc ggtcgccgac
       61 aacaagaccg ccgaaggccg cgcgaagaac cgccgcgtgg aaatcgaagt ggtcggtacc
      121 cgcgccaccg gcaagtaatc cccggttcgc cgcgaagtcg acaggcttcg cgcgattcgc
      181 gagaatcctg agacccgcct cgagcgggtc ttttcttttg cagcttccgg ttattcgcgg
      241 ggccgcgccc gcgaggcgct agcattcaga ccatgacgaa cgccgacccc caggaactcg
      301 agaaattcgg tgaactcgcc cacaaatggt gggacaccga aggcgacttc aagccgctgc
      361 accgcatcaa cccgttgcga ctggactgga tcgcccagcg caccccgctg cacggccgca
      421 gcgtgctcga cgtgggctgc ggcggcggca tcctggccga gtcgatggcc cagcgcggcg
      481 caagcgtgct gggcatcgac ctgtcggtca agccgctgaa ggtcgccaag ctgcacgcga
      541 tggagtcggg cgtcggcaac ctcgagtacc gcgaggtcgc ggtcgaggcg ctggccgccg
      601 agcgtcccgc cagcttcgac gcggtcacct gcatggagat gctcgagcac gtgcccgatc
      661 cggcctccgt ggtcgcgtcg tgcgcgcggc tggtcaagcc gggcggcatg gtgttcttca
      721 gcacgctcaa ccgcaacgcg aaggccttcc tgttcgcgat cgtcggcgcc gagcacctgc
      781 tcaagctgct gcccaagggc acgcacgagt acgccaagtt catccgcccg agcgaactcg
      841 ccggcttcgc gcgcgaggcc ggcctcgaga cgctcgagct cgccggcctg gagtacaacc
      901 cgctgacgca gcgctactgg ctgtcgggcg acacctccgt caactacatg gtggcgtgcc
      961 ggcgtccggc ggcgagctcg tgagcgcgcc cctgcgcggc gtgctgttcg acctggacgg
     1021 caccctgatc gacagcgcgc ccgacctggc cggcgccgcg aaccgcctgc gcgccgacca
     1081 cggcctggag ccgctggcgc tggaatcgct gcgtccgatg gtgggctccg gcgcgcgcgg
     1141 catggtgggc gtggccttcg gcgtcgcgcc cggcgacgag cgcttcgagg gcctgcgcga
     1201 cgccttcctg gcgcattacg aggccggcct gctcgaactc acgcggccgt tcgacggcgt
     1261 gctgcagatg ctgtcggcgc tcgaggccgg cggcatcgcg tggggcatcg tcaccaacaa
     1321 ggccacgcgt ttcacgctgc ccatcgtcaa gggcctgggc ctcgcgcagc gcgcgggcgt
     1381 ggtcgtctgc ggcgacacca cgccccactc caagccccac cccgagccgc tgctgcatgc
     1441 ggctcgcacg ctgggcctgg cgccggagca cgtggcctac gtcggcgacg acctgcgcga
     1501 cgcgcaggcc gcgcgcgccg cgggcatgcc catgctggcg gccacctggg gctatctcgg
     1561 cctcggcgag ccggcgcaca gctggggtgc ggagcgcctg gtcgatgcac cgtcccaggt
     1621 gctggactgg cttcgggcgc gcgcgggctg acgcgccgcc gacgcagctc gcctgcgggc
     1681 gaggcgtccg cgcctggcag ggttcgtgct tgagctgcgc gacgggcgtg tgggaggcgg
     1741 cgcgcgttat cggccaccgg ccggttcaat ttagggggtg tcctgccgag ttgtgacgac
     1801 cgtcacatga aaaccggcaa atcgggtaat tccgttacac cccgcgacag ccttgcgaca
     1861 tgcggactca ctaacatggt cgagttcaaa cggaggtatc ccatgaagaa gatcctggcg
     1921 atggctgccc tggccgtcgc gtccgtctcg gcgtccgccg cgaccaacct gttcgtcgac
     1981 ggcagcttcg agtccatcac gcagcccgcg ggcacgtgga gcacctacca cgtcgtgccg
     2041 ggctggacgg tgacgcaggc cggcagtttc ctgccgtcgc cgcacggcct cgagatccgc
     2101 aatgacgtgg cgggcagcgc gcaggacggc aacaacttca tcgagctgga cggctacgag
     2161 aacgaccgca tcacgcagag cttcgccacc accatcggga aaacctacga ggtgtcgttc
     2221 tggtaccaga accgcgccgg cgtcgccgct gcttccgagg gcttcgaggc cgtggtgctg
     2281 tcaggcggcg tgacttcggc ttcgctggtg ggcaatgccc cgtccgacgg ctggctgcag
     2341 gaaacgatgg tcttcacggc cggcagcaac ttcacgacgt tcaccatcgc cgcgacgggt
     2401 acctccgaca gcctgggcac gtcgttcgac gacttcacgg cgctggccgt ccccgagccg
     2461 gccacgatgg gcctgttcgc cgcgggcctg gccatcatcg gcctgtcggc gcgccgtcgt
     2521 cgccgcaact gatcgagtcg ggctgaaagc ccgtccgacc agggcgcccc gcagcgatgc
     2581 ggcgcgccct ttgtcgtttt ccggcctgct ctgcgcgacg gcagcggcga tcgtcccttg
     2641 aaggccggcg gcttgcccta aaatgcgctg tactggggcc gaccaggttt cgacgtgggt
     2701 acggatccgg cgcagggcat gccgagcacc agttcgctcg taaatccact ggaaacaaag
     2761 taactgcgaa cgactcttcg tacgctctcg cggcttaata cccgtgagcc tctcaacggt
     2821 tggccgatgg gccgggcact tgatcgcaag atcctgagct gagaggtcat tcacattggc
     2881 tggtctcctg tcgggctact gggcagggga tgagatcaag tagctggcgc catcggtagc
     2941 gtgttcctgc gcgaccgctg gtgcgagatc caaaccagag aactacgcat gtagaactgt
     3001 ccggcgatgg cttgcggacg ggggttcaat tccccccggc tccaccattt gcagatgtca
     3061 gctggttcca attggtgccg gatgatcccg aaaagcccaa gccgaccaac gacttgggct
     3121 ttttcattgg cctcgccggc tttcatgcga tcccatccgc tttcgcgacc cgcgtgaggc
     3181 tgcaggagcg acttccggag cgacggccct cctggacagc gtcatgccga ccatggatgg
     3241 catgtcggcc acgcacctgc gccaggatgc cgatctgagc aaggggccgg agatcgccct
     3301 ctcggcttca gccggcgccg agcagcgtga cgctggcctg caggccggca tcaacgtctt
     3361 cctggccatg cccgtgtccc tggaagcgct gcgcgcgcat gtcggagagc agccgggcct
     3421 tcaatggatc gcaccggcaa ccgggacgga ccgaatcgct cctcgttccc gtccaggtcg
     3481 gttgcaccca tacccgtcta cgtatgcgtc cggggcgtct cacgcgctcg tcggctgggg
     3541 ttgcgagccg gtcgagcgtc aggcgtcgtg ttccgccgtg cccgcaggat catcaccgtc
     3601 gagctcggcg acgaagcggt acccgatgcc ctggtgggtg cggatgatcg cgcccgcgtc
     3661 ggccccgaca tcgagcaacg ccttgcggat gcgcatgacg gcggcggcca gcgaaccagg
     3721 ttgcacgaat tcgtctttcc agatgtggtc gaggagttca tcggtcgaga ccacccgttc
     3781 gcgatgctcg atcaggtaga cgaggacgtc gaacgggcgc ggctgcagcg tgcgcggctt
     3841 gccgtcgacc aggacttcgc gccgggccgc cgacacctcg cgccggccga acaggaaatc
     3901 gccgcgcctt cttgcatgcg agcgtcggtc gcgcgccggg gcggccgccc gcgccagcaa
     3961 ggcgttcgcg tcgttggggg cagcggcatg caacgggatc atgttcatcg gctggtagag
     4021 attcgaagtc gtcatgatgt agacatgcga ctacatcgac gccgggatgg ccggcgcgac
     4081 gaatcgatat cccacgcgct gataggtccg gatgacgtcg ccgtggttcg gacccagggc
     4141 gcggcgcagg cgcaggacgg cggccggcag cgaccagcgc tgcaccgagg cgtcccccca
     4201 gacttgcgac agcagttcct cgctggtcac cacccggtcg ccgttctcga cgagatagac
     4261 gagaagatcg aagggacgcg gctggagggg ccggcggacg ccgtcgacga acagctcgcg
     4321 ccgggcggtg cgcagttcgc agcgcccgaa tcggatgacc ggttcgtggc ggacgagcgc
     4381 gagcgtttca tcgacatccc ccggcgccgg gggcgcgcca ggaggcaagt ggaccggaac
     4441 gacgaacatg actcggctcg gatggtggtg gtgtaggtgc cattgtgtag tgatatgtct
     4501 acacgtgtct acagcgccga ggcatccgtt ccggaacttt tttcacgcag gcgcgggccg
     4561 cgacgctttc cttcaggtgg cgtcgagcag gacgcgagcc gggcggccga gttcgagcgt
     4621 catctggagt ccccacgcct gggccgtccc ttgccagtct gccggcagcg tgcgcgcggc
     4681 gatctccatg gcgtccacgc attcagggtc gaactgcgtg ccgcgtcggg cgaggacgtg
     4741 gtcgagggcg gcctgcaggg gccaggcggg cttgtacggg cgaacgctcg tcagcgcgtc
     4801 cagcacgtcc gccatcgcga cgatgcgtgc gcagcgcgga atcgcggtgc ccgcgagtcc
     4861 gaacggatat ccctggccgt tccaccactc gtgatgggac agggcgacgg cgatcgccgc
     4921 ggtcgtcgtc tcgtcgccgt ccggacgggc ccgcgccatg aggcgcttcg cgcccgcgat
     4981 gcagtgccgc tccaccaggc agcgttcgtc cgcgtcgagc gcgcccggtt tcgcgaggag
     5041 tgccggcggc aggtccagct tcccgatgtc gtgcgtcaat gcggcgacgg cccagtcacg
     5101 cgattcgatg gccgagacgc ccagcaccgt gcacaggcgc gccgtgagcc acgccgtgcg
     5161 cctgccgtgc cgatccactt cgatgctcat gcggcatgcc ctggttgacg aggaagattc
     5221 aatgtaggaa acatcgccgc gcgacgcatg acccggttct gaccgcggcg cgccggcccg
     5281 ggaaagcgtg aattgcctcg cgaaaaccgg acgctggccc gcgattgcgc gtgtcggccg
     5341 cggtatcgca cctgtgccgg caggcatgca gccggagccg ccgccgtgtt tctgcaccga
     5401 tgcgctgcag ggcctgtcgt cgtcgtcacg cgcatccgtc caggtccttg ccgtgcgtcc
     5461 tggccatgtc gcagatgtcc acccgtccgg tgcgccagac gcgcaccttg tactggcggg
     5521 tggcatccga tcgcgccacc aggtaggacg tcacgccgcc gaggtccgcg ccgggttgcg
     5581 cgcaggcgat ccgcgtgcca tccggcgtgt ccggtgtcga catctgcagg ccctgcgcat
     5641 cgaagcacac ctgcgcgggc ccggtgaggg tcacgcggtc gcgggcggac gccgtcgtcg
     5701 ccaggatgac gtccgcgccc cgggcagctt gcgggcgcgg ggtcgacggc aggaacttga
     5761 cgagccagtt gcctgcatgg actgcggcca ccgcatcggt ctgcggcgcc gcgtccgtca
     5821 gcatgaaggc ggcggggcgg ttgcgcgtca cggcgctggc gcgcgcgagc cgcagggcgc
     5881 cggccagctg ctgcgcggtg ctgcgggtgt ggatgtccgc gatcgcccgg tcgaacgacg
     5941 gcacggccag tgcgaggacg attccgccca ccgacagcgt gagcatcatc tcgatgaacg
     6001 agaagcccga tgcgcggccg cgcgcccccg cactggaaga accctggaaa tccgtcgtca
     6061 tggcgcccat gcacccgctc ccttgccgcc ggatcgccat cgatccggac ctgcagagga
     6121 tggtcgtgcg cggcacgacg caggtcatga tcgcgacatg accgggcgat ggcgccgaat
     6181 gcgaccgcgg ccggggcgtg acgcttgtca ctgtcgcggt cgagggtcgg acgcgcgggt
     6241 tgcgcccggt gacgcatggc cggccggggt tcacaatctg gccatcacca gccgcctttc
     6301 gatcgcgcaa acccatggac cgggcgatgc ccctcagctc gcagccgacg acagcgccgc
     6361 cggtcgacgt cctcctcatc gaggacaacg ccgccgacgc ccgcttcttc ggcctgctgc
     6421 tggagagttc gccggccgcc ggcaactttc gattgcgcca cgccgcgacg ctggccgccg
     6481 gcgtggcgat gctcggcgcg cggcggcccg acgtggtcgt gctcgatctg ggcctcgggg
     6541 aatccgaagg cctggcgacg gtgcagcacc tggtcgcgtc cggcgttcgg ctgccggcgc
     6601 tggtggtgtt gagcgggctc gatgacgagg acgtcgcggt cgaggcgctc caggcgggag
     6661 cgcaggacta cctgatcaag ggccagatcg atggccgtca actcgcacgc gcgatccgct
     6721 atgcgatcgg gcgtcaccag gccgaacacg cgatcgccga gcgcaccgcg gagctcgaac
     6781 gctcgcgtga gctggtgcgc agcctgggtg cgcgcaatgc caggctgctc gaggaggagc
     6841 gccggcgcgt cgcgcgcgag ctgcacgacg agatgggtca gcaactggcc gcgttgcgca
     6901 tggagatcgc cgtcctgcgg caggggcggg tggcggccgc ccaggcgtcg tccgatgccg
     6961 cgctgggcat gctgctcggc cgggtcgacg gcctggtcgt cagtgtccgt cgcatcgtgg
     7021 cggagctgcg gcccccggca ctggacggcg ggctggccgc ggcgctggac tggctggtgt
     7081 ccgacttcga gcggcagacg cagctggact gcgaggtcga tgtcgatgcc tgcgcgagcc
     7141 tgttgtccgc ggacgcggcg gcgatggtct ttcgcatcgc ccaggaatcg ctgaacaacg
     7201 cccgacggca tgcggtggcg tcgaaagtct cgctgcggct gcagcgcacc gacggcggct
     7261 gcgagctgcg agtcagcgat gacggcggcg ggttcgatcc ttcggagcgg acggccggct
     7321 tcggcatcct gggcatggag gagcgagccc gggcgctcgg cggcagcctg gacatcgaca
     7381 gccggcctgg ccagggcacc gaggtgcggc tgcggctgcc gctgtccgaa gcccgcggcg
     7441 tcttgcctgc tgcgcggccc gactcctgaa tccgcgcgtc tgcgttggcc ggtccttcaa
     7501 cgcgatccgg aggccggccc cgactcgggc agcggcagcc gaagccggac ggtgcagcct
     7561 tggcccgtgg cgctggtgat cgcgagggtg ccgcccaggg cgatggcgcg ctcttccatg
     7621 ccgaggatgc cgtatcccga tgtccgggcc gccggatcga atccgacgcc gtcgtccgtg
     7681 atcgtgagtt cgcacccttg cccggcccgg gccaggcgaa gccaaacgcg gccggcgcgg
     7741 gcgtgcctgc gcacgttgtt caaggactcc tgcgcgatgc ggaacaccat caccgcggcg
     7801 tccggcgaga gcagcgtcgc gcactcgtcg atctcggcga cgcaaggcag gtcggcgtgg
     7861 cgctcgaacg cggcgaccag ccagtccagc gccgcagcca ggccgccgtc cagagccgga
     7921 ggccgcagct ccgcgacgac gctgcgcatg cccgtcacga ggtcctccac gcgaacgagc
     7981 agcatgccga gcgcggcttc cgtggacgac gcgtcatggt ccgaggtgcg ccgcctgagg
     8041 acggagatct ccatccgcag cgccgccagc tgctggccca tctcgtcgtg cagctcgcgg
     8101 gcgacgcgct tgcgttcttc ctcgagcagg cgcgtgttgt gcacgcccag gctgcgcacc
     8161 agctcacgcg agcgttcgag ctccgcggtc cgatcggcga tggcctgctc gagacgggtg
     8221 cgcatcgcgc gtgcacgcag ccgcagaagt tgcatcgaca ggaaccacgc ggcggccagg
     8281 ccgagcacgt acgtgatgcg cgccggagtc gatcgccacc acggcggggc gaccgtgaac
     8341 gcgagctcca gcgggatgcc ggcctgcagg agcggcgcgt tcgcggccgc gcgcacctgc
     8401 agccggtagt cgccggcctc gagcgactcg agctgcagca cgttgccgac gaagccttcc
     8461 caggtctgcg cgtcgttcaa ccggtactcc aggcgcatcg agcggccgcg cgcgatctgt
     8521 gccgtctgga gcgtcagtcg cagccggcga tcgtcccagt cgacgcgcgg tggcatcgcc
     8581 cgcgcacccg gacggtcccc gaagcggacc tccgcgacgc gcaagcgcgg ggcgtccgcg
     8641 gcaagccggc ctggaagcac atgggtcacg cccttgctcg aaccgatcca gacgccgccg
     8701 tcggcgtcga agtggatggc gccggtgtcc acgtcgttcc agagcagccc gttgtcgcga
     8761 tcgagctgca tccagcgcgc gtcggtatgg acgaacacgc cgcgatcggt gccgacccag
     8821 acacggcgtt cgctgtcggc gccaaggaat tcgatgtctg ccgtgccgag ctgcgacgga
     8881 tcgatgcgct gcttcagtcg cgccgttccg tccacgaccc ggtacgtcgc cagccccttg
     8941 gagtcgtgcg cccagaactc ggacggaccg gcgaatgccg ccgctcccat cacctcgatc
     9001 gggttgccgc gttcgtcgcg caagggctcc cagccgccgc gatcgatgcg ccacgcgccg
     9061 ttgcccacga ggaaccactc gcgttgtccg tcgctcacca ccgcgacgag cgagctcgga
     9121 tcgggcatgt gcgcgggcaa cggctgacgc accgggcctg cgggggcagg caccagtcgt
     9181 tcggccgaac ttgttcccga cagcacgatc gaatgggcgc cctgcagaac gacgtcgatc
     9241 gcgggatccc ggaaggcgac gcgggcgcgg cgcatgccgg gcgccagcga catgacgtgt
     9301 cccgcgtcga cccaccagac agtgccggcg tcgtcggttg ccaggctgcc gcccggcgtc
     9361 ggtgcgaaca gcggctccac ggcgacgaat cggcgcgtcc gcgggtcgag cgccgccagc
     9421 ccgcgtgacg tggcgacgcg catctgcccg gcgccgtcgc gcgcgaacga ccagaccacg
     9481 ggcgagggca ggcccgacac cgacgtccag tgctcgacgc ggccatagcc ggtccacaga
     9541 tgcacgccac gcccggccga gcccgtccac agctctccgt cggcatcgac gagcaaggcg
     9601 cgcacggccg tctccgggat gccttcgtgc ggcgtccagc gtcgccatga acggccatcc
     9661 cagcgggcga cgccgtcctc gaccgcggtg aggatgcgcc cttcgagatc ctcggcgatg
     9721 gcgatggtgc cgggccacag cgtcgcggac ggcgcatcga cggtctcgaa tcgatcggca
     9781 tcgggcgcca gccgcgcgag gtgccggtcg ccgcgggccc agacgctgcc atcgcgggcg
     9841 gccagcaggc tggcccaggt gtcggccggc aggccctggt cggggcccca ggcccggagc
     9901 tgcccgtcca gccagcggca gagcccgtgg ccacagccga accacagggc cggtccgacc
     9961 gcgcgcagcg gccccccgtc ggcgtcgtag gcgccggcgt aaggttcgta cgccggcatc
    10021 gtcatccggc tcgcgaccac cttgtcgggc aggcggggcg gcacggtgat cgtccagatg
    10081 cggctgcgcc ggtccatcgc gaacacggcg ccgtgctcgt cgaccgcgac gcgctgcttg
    10141 ccccaggccg ccagccccac gccctgcgga tcgacgatgg cgctccagtg cgcgacgccg
    10201 gcatcctcgc ggcgcaggta caggcccgtg tcggtcgcga tccagagccg gttgaaccgg
    10261 tcggcctgca ggtcggtgat cgtggccgcc gcgcccttgg gcagagtctc ccggcggatc
    10321 cggaagccgt cgaagacgaa caggccgttg tccgtgccga tccacatcgt gccgtcgggg
    10381 ccctgcgcga ggcactcgac ggccatgctg gtcagtcccg cgctctcctg gtagggctcc
    10441 atgtccgcga tctgcgcgtg gcccacgccg gccgtggcgg ccagcgtgac ggcgacgacg
    10501 atcgcgcgca gcctctcagt cgaccaaccc atggcgcagc gcatagcgca ccaggtcgct
    10561 gaggctggtc tggcccagct tctccatgag ccgccccttg taggtgctca cggtgttcgg
    10621 tgcgagctgc agttcgtcgg cgatgtccat caccgagcgg cccgtgacga gcgcgacgag
    10681 cacttgccgt tcgcgcgtcg acagctgctc cagctgctcg cgcgggctcg ccggctcgcc
    10741 gcggatcagc gcgccgctga gcgagggatc gacgtagctg cggcccgcga gcacgtggtg
    10801 gatggcatcg accaggagct cgggcgaggc gtccttcgtg agatagccga gcgcgcccgc
    10861 ctcgagcgca cgccgcgcga tgtgcgggtc ggcgtgcatg gtcagcacca gcaccggcaa
    10921 ccggggatgt gccgcgacga cacgcttgat gaggtcgacg tcggtcgcgc ccggcatgag
    10981 caggtcgagc agcaccaggt cgatgccgcc gcgcttgagc cgtgcgagga cctccgcggc
    11041 cgagacggct tcgccggcca cgaccagccg ctcgtccagg cccagcacct tgccgatgcc
    11101 ctcgcgcacg agcgcatggt catcggcgac gaggatgcgg tgcttctgga cggctgccgc
    11161 ggcagaggtg gacatggcgg gaactttcga cgcagggctg gaaggtcgcg acagcgggcg
    11221 cgcttcggat ggaatgtaga tattacacta cgcttctttc cttttcgccc cgggccgggt
    11281 gcgcgctgcg tcgtccggca cgagccgggg tcgcggccag cggcacccga tccatctcga
    11341 tgtagtcggt caggctgtcg ccgaaagccg cggcctgttc cgggcccacc ggcggcggca
    11401 tccgcaccca gtgcgaagga cggtcgccgt gcttgcggcg caggacgagt tcgacccgcc
    11461 gccgcaggac gacgaggccg ggcccgaaca gatcgtctcc cagttggtcc agcgcccgtg
    11521 cggcatgccg cagcacggcg gcctccaccc gatggatgtt gtccgcgctg tccgagccga
    11581 gcacccgctc cagcagcgcg agcgagggcc agacgcggcg cgcgccggcc acgtggtcca
    11641 gcaggacgcg aagctcgtgg cgcgccgccg acgcgtgggc gccgggcatc ggcggcagct
    11701 gggggcgccg gacgggtcgt ggccgcggcg ccggtggcgc ctgccgcggc gcgccggcgg
    11761 gctcgagcgc cagccgccac gcaagcccgc tgcgccgaag acggatgcgc cgctgcaggt
    11821 actcggacag ccgcagccag agacgccggg cttcggtttc ggatttccgc gtttcgggtt
    11881 tcgacatctc gggagtggtt catcgcgccg tgccggcgca ggcatttcgt cacgagttca
    11941 ccgcgccgca gggttgcacg cgccggaatc ggtatgtaga gttatatcta caacgtagat
    12001 ccatgtctat aactgcgatg ccatccatgt cttccaaccc gggcaaatgc cgcacgaagc
    12061 gccatgccgg cttcacgctg atcgaagtga tgatcgccgt cgcgatcgtc ggcatcctga
    12121 cggcgatcgc cttgccgtcg tacaggaact acgtcatccg cggcaagctc gtcgcgggca
    12181 cgaacgcgct cgcgaacatg cgcacgcaga tggagcagta ctaccaggac aaccgcacct
    12241 acgcgaccgt ctccgcgccg gccatcgtga cgccctgcgt ggccaacgcc gtcgtcgcga
    12301 gcaacggcac cccgttcacc gtgggctgct cggccgcgtc ggacgttccc acggcgacga
    12361 cctacacgct gcgcgccacg ggcaccggca cggtcgccgg cgccgtgtac acgatcgacc
    12421 aggccaacaa catggccacc gtctccttcc cgacctcgtg gggcagcgtg ccggcgaaca
    12481 acgcctgctg gatcatgcgc aagggcgact cgtgctgagg cggccgaccg cgatgccgcg
    12541 cggcttctcc ctcatcgagc tgatggtgac ggtggcactg atcgcgatcc tgctggtggt
    12601 cgccatcccg gcctacggca cctggaccgc cgacgcgcac gtgcgtgcgg ccgcggagtc
    12661 gctgaccaac gccatccgcc tcacgcaggc gacggccgtg gccaagggac ggacgagcct
    12721 gttcgcgctc acgacggacg cgcagccgtc ggtcaactcc gcgccggccg cgtatgcgcc
    12781 gaactggttc gcgaccctga acgtgctcgg cggctccgac gagaccaagg cttcgctggg
    12841 cctgatcctg aagtcgacgg agggcaccca gcaccgggtg acgatcagcg gcccggcgct
    12901 cgtctgcttc aacgcgctcg gccgccagac gagcctggcg gccgcggcca acagcctgtc
    12961 gaccgcctgc acggcgggcg atcccgcggt cttccaggtg tcgcgcgacg ccggcgcgtc
    13021 gcgctacttc aaggtgctcg tctacgccgg cggccgcgtg cgcatgtgcg acgcggccaa
    13081 ggcgatctcc agcgccgatc cggacgggtg ctgaccatgc agcaacgcca ccgcatgccg
    13141 ggcctgcgcc gatcgacccg cgggttcacg ctcatcgagg tcctgatcgc gatcctggtc
    13201 ttctcgttcg gactgctcgg ggccgccgcg atgcaggccg tggccgtgca ggccgcgacg
    13261 cagaacggca accgttcacg cgccgcgctg ctggccaacg agatggtcag cacgctgtgg
    13321 gccacgcagt cctccacgcc ggcgccggcg gacctcgccg cgtggacgtc gaagatcgcc
    13381 acgccgtcgg cctccggact gcccaacggc tccggcacgg tgaccgcctg caccggcgtg
    13441 gccaattgcg cggtgatcac ggtcacttgg aaggcacctt cggccaagac cgccgccgcg
    13501 tcgaaccggt acacgaccac cgtggtgatc caatgaagcg cagcccttcg ccgcaagccg
    13561 gcgtgaccct gatcgagatg atggtcgcgc tggtcatcgg catggtgctg tcgatcgccg
    13621 tgatgatggt gttgtcgaca ttcgaggggc ggcggcgcac gctcggctcg acctccgacc
    13681 tggaccaggc cggcgcgctg gcgatgttcc agctggatcg ctggatgcgc agcgcgggca
    13741 cgggcctggg ccagggcagc agctttgcct atggctgcac gttgttcgcc gccaggtcgg
    13801 gccaccaggt gctgccgctg accgggccct tgccggcgcc gttcgcgtcc gtcgatccgg
    13861 gcacgagcgg cgtgttccgg ctcgcccccg tgctcatcct gcccgggcag acgatgccca
    13921 acatcagcgg caaggcatcg gacgtgctcg tcgtgatgtc ggccggcaac gatgcgtcgc
    13981 aggtcccctc gccgctgtcg gcgccgccgg cggccgacca cctcgccatc gacaacctga
    14041 ccgagttctc gccgggcgac ctgctgctgg tggcggacca ccaccagtcg ccgggcggcg
    14101 gggcggcgaa ctgcatggtc tcgcaggtgt cgtcttcgct gaccacctcg ggaacgggca
    14161 cgacgctgcc cctgggcgga tcggacagca cttacgccag cgcgagcatc ggcagcgcgg
    14221 cgatcacgag cctcaccgac ggtccggtcg cgttcgacct cggggcgccg accgcctccg
    14281 gcaccagcca gcccccgtcg ttccagctca tcggcgtggg cgacaacgac accctgtacg
    14341 gctacgacct gctgcaggtc tcgggcagca gtgcgcaggc ccaggcggaa ggcgtgttcg
    14401 agatgcacgc cgtctatggc atccactcct ccgcgaacgc cgacaacaag gtcgaccagt
    14461 gggtcgacgc gggcgcgacc agcgcgtatt ccgtcgcggc cctttcggac ggcagcgtcg
    14521 gtgcgacgac gcggctgaag aacatcctgg ccgtgcgcgt ggcgctgatc ctgcgcacgg
    14581 cgctgcccga gaaggacgcc gtcacgccgc atgcgctgac catgttcacc gaccttcccg
    14641 gcctggccat cacgcgcacg ctgacgtcgg cggaacagca tttccgctat cgcgtcgtcg
    14701 agacgaccgt ccccattcgc aacaacggat tctgatgagc gcgccgaccc tttcacatcg
    14761 agcccggcgc ctgcgcggcg tgcggacact ccggccccag cgcggcatcg tcatggtgtt
    14821 cacgctgatc gcgctcgtgc tgatgctgat cgcggtcgcg gcggtggtgc gcagcgtcga
    14881 cacgtcgtcc ggcgtcgtcg gcaacctggc cttccggcgc gacctgacga accgggcgga
    14941 gctggcgatc gcgcaggcga aggcggccct caacgcgggc ggagcggtct atgccgagag
    15001 cgcgcgccgc gccgacctcg cggcggcgca ctattcggca agccgcctcg cgtcgccccc
    15061 gggcggcatc ggcgtgccgg cagtcctcgt cagcaacagc gcctacgact cggccggcta
    15121 ttcgtgcatg aacggcgccg gcgtcacgct gcccaattgc gccacgccgg gggccgacgg
    15181 catcgtgatc cgctgggtca tcgatcgcca gtgcgtcgcc gggacatcgg cgttcgggac
    15241 gagcgcgtgc gcctacctga acaacgtcac ccctcccggg ggcaccggcc actccctgac
    15301 acgcaagccg cgcggcggcg gccgctcggt gtttcgcatc agcgtgcggg tcaccggccc
    15361 gcgcaatacc gaagcctata tccagaccac cgcgggctga ccccatgcca tcgaccctgc
    15421 ttgctcctgt ccgttcgcga ctgctgtcgc cgatcgcctt cgtcctcgcg ctgctggcgg
    15481 cctccgcggc ggtcgcacaa ggcgtcgacc tcgccgacaa gccgctgttc tccaccacca
    15541 gcgttccggg caacctgctg ctgtcgctgt cggtggaata tccgacggcc aacacctcgg
    15601 cctacttcag cacgacgccc tacgcgtccg gcacgctgta cctgggctac ttcgacggcg
    15661 cgaagtgcta tcgctacctc tacaacagcg cgtccccggc cagcagctac ttccagtcgt
    15721 acggcgcggc gtcggccagc caccagtgca cgagctcgag ctcgctgccg ctgtggagcg
    15781 gcaactacct gaactacgcg gcgatgcagt cgctggacat cttccggtgg gtgatgacgg
    15841 gcggcaaccg cctggtcgac accgccgggc ccaacggcat gaccatcctg cagaaggcaa
    15901 cgcattcggg ccagggcgcg gcgggcgacg cccccaacaa gagcatcccg tacgcggacc
    15961 aggcgggcgc gacgcccttc aactggggca acggcgtcaa gtcgcgcatc tggggcgcgg
    16021 ggatcaagct ctggctgacc ggttcgggcg acctcaaccc caccaacatc accgccgtcg
    16081 actaccagga ctcgctcatg gccaacggct cgcccgatgg cgcgaccgtc tatgcgctgt
    16141 acatgaacgt gcgcgtgtgc gatgccacgg tgagcctcga ggccaactgc gtcgcctacg
    16201 gcagccagtc caagcccgag ggcctgatgc agcagtattc cggctcgctg cgctattcgg
    16261 cgttcggcta cctcaacgac agttcgccgt ggcgcgacgg cggcgtcatg cgggcgcgca
    16321 tggcctacat cggtccgaag atcccgcagc cgggcgcgac cgccacgatc aatccgaatc
    16381 cggaatggga tgcgaccacc ggcgtcatga tcgtcaaccc ggacccggcg gacgcggccg
    16441 ccaccaacac gtccaccaac aaccggacgg cggtcagcca gagcggcgtg ctcaactacc
    16501 tgaacaagtt cggctacgcc tcgggcacct acaagaccta cgaccccgtg agcgagctct
    16561 actacgcctc gctccgatac ctgaaggggc agacgaacgt cgccgcatac tcggacatgt
    16621 cgggcgcctc ggccgccacg gccagcacct ggaccgacgg ctttcccgtc gtgacggact
    16681 ggtcgaccga cccgatcatc tacgcctgcc agaagaactt catcctcggc atcggtgacg
    16741 tcaataccca ccgcgacggc aatctgcccg gcagccctgc gagcgacttg gagccagcgc
    16801 aaccgcccga ggtgcgcgcg gacgcatcgg gggtgtcgcc cgtcgacgtc ttcgcctcga
    16861 ccaacctggt cggatcgatg gagggccaga gcaacctgtc ctggcgctgg gccaccggcg
    16921 acgaggggcg gttctacatc gccggcctgg cctacgacgc ccacgtgccc atcgccggca
    16981 agggcgtgcg caccttcacc gacgcgaaca ccaacgtgac gacggtgagc accgccagca
    17041 cctactggct ggacgtgctc gagtaccagg cctacgtcaa caacaaccag tactactacg
    17101 cgaccaagta cggcggcttc acggtgccga ccggctatgc gtacggcggc gcgttgccga
    17161 ccgacagctg gtacaacagc acgaacacgc tgggcagcaa caagcagccg gacaactact
    17221 tcaccgcgag caacgccacc ctgatgcgca gtggcctgac caatgccttc gcgaagatcg
    17281 tctcggagaa caacgcggcc aacggcaccg cgctgtcgct gccgacgccc aacgtcagcg
    17341 ccagcggctc gatgagctat tcggcgacct acgatcccac gtcgtggacg ggccaggtca
    17401 tcggctcgac ggtcgtcttc aacgcggacg gcacgtccac gctcacgcag cgatgggatg
    17461 cgcgcgcgct gctcggcgac ccgaacgtga cggccgccac ccgcaagatc gtcacctgct
    17521 gcgcgctgaa cgcgacatcg ccgggcctgc ccttcacgtt gaccagcctg cgcgcggcgt
    17581 cgctcgactc gaggacgtac tacgcgtcgt tcgccaacgt gccgggcgtc gcgtcgtcgt
    17641 cgcagtcgat cgccaactac atcgcctacc tgcggggcga cacgagcaag gaagtcgcca
    17701 acggcggggt ctaccgcacg cgcaagttcc gcctcggcga catcgtcgac gccaagcccg
    17761 tcaccgtggg cccgcccaat tccgggttct acgacaccta caacccgggc tacgcggcct
    17821 acaggacgca atacctgaat cgcaggaacg tggtgtacgc gggcgcgaac gacggcatgc
    17881 tgcacgcgtt cgacggcacg gtcgacaaca ccaatccggt gtcgggcagc gagctgttcg
    17941 cctacatccc gagcttcgtc tacggcaacg cgagcactgc cgcggccacg ggcctggcct
    18001 cgctgggcaa tcccgccttc acgcatcact acctggtgga cgccacgccc ggcgagttcg
    18061 acatcgacct gaacaggacc agcggcgccg cgggcgaccc gaagtggcgc acgctcctga
    18121 tcggcggcct gggcaagggc ggcaagggct attacgcgct cgacgtgacc gacccgacga
    18181 cctggagcga cgaggcgacc gtcgcgagca aggtgatgtg ggagttcacc gactctcgca
    18241 tgggctacac gtacgggacg cccacggtgg ccaagacgaa gaagtacggc tgggtcgtca
    18301 tcttcacttc cggctactcc aacgcggacg gccgcggctg gttcttcatc gtgaacccgt
    18361 ccagcggcgc gctgctcgag gcggtggcca cgccggccgg cacgggatcg acctcgtcgc
    18421 ccctgaacat gggccaggcg accacctaca tcgagaacta caccgacatg acggccgacg
    18481 cggtctacgc aggcgacctg cagggcaacg tctggcgcct ggacgtgagc gcgacggacg
    18541 tcacgctgcc ctatcccacg cccacgcgga tcgccaggct gaccgacgac cgcggcaacg
    18601 cccagccgat cacgacccgg ccgctgatcg agatcgcgcc gaactccacg acgcgctacg
    18661 tgatggtcgg cacgggcaag ctgctggccg acagcgacat cgccagcggc gcgcagcaga
    18721 gcttctacgc cttcgtcgac ggcctggcga cgtcgggcgc gttctacggc gcgccgggac
    18781 aaccgctgcc caccggcgtg tccttcccga tcacgcgcag ccagctgaat gcggacacgt
    18841 cggtgctgga cggcatcggc agctcgccca gctccgcgat gggctggtac ctcgacctgt
    18901 cggtcagcgg cggcatcgcc gagcgggtga acgtggatcc gatcgccagc cagggcgtcg
    18961 tcgccttcat cgggaaccag cccaacgggt cggcctgcag cccgagcggc aacggaaccc
    19021 tgcacacgct cagcatcgcc gccggccagt ccctgttgct gaacaccgac ctggtgactc
    19081 cggtcgcctc catcgcgatc acgggcatcg gcaccgacg
//
LOCUS       DATCRO010000622        10744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_153348, whole genome shotgun sequence.
ACCESSION   DATCRO010000622 DATCRO010000000
VERSION     DATCRO010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153348"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VIN75_17000"
     CDS             complement(<1..476)
                     /locus_tag="VIN75_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8975919.1"
                     /translation="MSSTNDPLRADFYSADSYRQEESVGAVLKGVIASITHQVDRRLV
                     DHDLTHAQWVPLFKLAHGECGTVAELARTLQSDPGAMTRALDRLEAKGLVARVRSSED
                     RRVVNLELTDAGREVAAVVPAVLAEVLNLHLAGFSHDEWRALLSMLKRMRANGDALR"
     gene            609..2477
                     /locus_tag="VIN75_17005"
     CDS             609..2477
                     /locus_tag="VIN75_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8975920.1"
                     /translation="MKSASERAKAASPRSLSGLLPFLRPYRGRILAALLFLVAAAITT
                     LAFPVVLRKLIDGGLGLSGAASLGSGATSAEANGARLLALRDHFLALFGVGVALGVFS
                     ALRFYSVTWLGERVTADLRNAVYRHVVKQSPAFFETTATGEVLSRLTTDTTLVQQVVG
                     SSLSMGLRNGVMGLGAMVMLIVTNPYVMTQVLGILLLVVAPSMAFGRRVRKLSRASQD
                     RVADSSAIAAEVLNAIPVVQSYVQEHREAARFEVSTEHAFDTARKRTTVRSMLVAFII
                     SATFGALLWGLYQGTQAVIAGRITAGHLGETVVYVMLLVSSVAVLSEVYGDLLRAAGA
                     TERLMELLAAVSPVADPASPRTLPPTGSGSSVELRHVQFNYPSRPMHAALADYSLRVR
                     PGETVALVGPSGAGKSTVFQLLLRFYDTGSGAVTVDGVDVRETSLHELRARIGIVPQD
                     STVFSANAMENIRYGRPDASDDEVIAAARAAFADEFIRRLPEGYDTFLGERGMRLSGG
                     QRQRIAIARAMLKNPPLLLLDEATSALDAESERMVQAALEAAMADRTTLVIAHRLATV
                     QKADRIIVMEQGRIIEEGNHQELMQRDGLYAKLARMQFNTEPLAPDDETAASEEAA"
     gene            2493..2930
                     /locus_tag="VIN75_17010"
     CDS             2493..2930
                     /locus_tag="VIN75_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975921.1"
                     /translation="MSTPPEDIDALFDLPPPAAPVLPASPLPGLPPPSADSRKEQRVK
                     VSWPARVQLPGGRVIELRVRDLCESGVGLLTEHHIPPATVLNFAVAVPGLNEGGRMTP
                     VSGTIKTTYVVVKGVDLFCGGTWVQLPPDGRDLLDKWIRKLRR"
     gene            complement(2969..4459)
                     /locus_tag="VIN75_17015"
     CDS             complement(2969..4459)
                     /locus_tag="VIN75_17015"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEY8975922.1"
                     /translation="MTIVIRRRAPLMALACFVPLAASASTACDHLLKQFGSQLADATC
                     VDSSDLTTTNPDTTPANNAIASLPAFAFTPQTDRATISPSAAKRDPITKVVPGIQLNG
                     RIASDPTGQARFLLRLPKDWNGRLVVAGASGTRSEFNGDFAWSDYVVQKGYAYASQNK
                     GVLNLQLTTAADPLGCRLNPVSTTFVDFYDNGPDQQFARWQEYMIKAAQLARQGVASN
                     YGRPPRFTYAVGTSNGGYQVRRAMETAPELFDGGVDWEGTFVDEHAPNLLTDLPAAIL
                     NYNDYAASGLDPNSTAAKNMRAAGYPPDIVASPTSTLWTSYWAQFWEVTQCQWQKRLD
                     PTYDTYGSGTGNYNYVARLSASDVGANVASFATTGRIQRPLVTVAGTMDALLPIDHHA
                     RAYARKVAAAQSRDRDHDGWGPRHDERDAAYRLYEVQNGNHIETYQDSFPQLALIQPY
                     AHAAFDLMVAHVETHAALPASQCIPKGGAVSASPSQPGHCAALYAP"
     gene            complement(4584..4967)
                     /locus_tag="VIN75_17020"
     CDS             complement(4584..4967)
                     /locus_tag="VIN75_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975923.1"
                     /translation="MIAIAGGLLAALGILAGALLGAAAFGLAAAGPSLWILFPLLTLV
                     GWFLLVVSDVDPLRGTPTRVVASALLLLALLAAVALVAGGAGLLAIQGTAGTLALCYL
                     LVLGGVLGATGTAAYGRKSGPPQHS"
     gene            complement(5041..5916)
                     /locus_tag="VIN75_17025"
     CDS             complement(5041..5916)
                     /locus_tag="VIN75_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY8975924.1"
                     /translation="MDDAILTCALTGVLTDPAQHPVPVTAAQMAAEARDAFGAGASIM
                     HVHVRRQEPGQGHLPSWDPAVAAEVCDAIRAACPGVIINLTTGVIGKDISGPLACIER
                     VRPEIAACNAGSLNYLKLRSDGRWAWPPMVFDNPVAKVQQFLDAMEAAGAHPEFECFD
                     VGIVRSVAMFVKAGMVKPSMGTPEVNLVMGVASGMPCDADLLALLPRWIAPGAVWQTT
                     LIGREEIWPVHQKTAELGGMLRTGLEDSFYLPGGERASGNGPMIAALARCAERAGRRI
                     ASPARARELLGLARV"
     gene            complement(5927..7924)
                     /locus_tag="VIN75_17030"
     CDS             complement(5927..7924)
                     /locus_tag="VIN75_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8975925.1"
                     /translation="MNRILVANRGEIACRVIRTARSLGYRTVAVFSEPDAGSPHVALA
                     DEAVALGGQAAADSYLRIAAILAAARRTGADAIHPGYGFLSERADFAQACVDAGLVFI
                     GPPASAISAMGDKAGAKRRMLAAGVPCVPGYLGDDQSDPALVAQANALGYPLLVKAVA
                     GGGGRGMRLVHAQAGLADALAGARREATSAFGDGTLMLERLVAEGRHIEIQVFADAHG
                     NVVHLGERDCSAQRRRQKVIEESPSPYVDAAMREAMGRDAVAAARAVGYVGAGTVEFI
                     VDEQRRHFFLEMNTRLQVEHPVTECVTGLDLVEWQLRVAAGEPLPLRQDQIRWSGHAV
                     QARLYAEDPYDGFAPQTGRIAYWRPARALRAGRADAGTQGVPVATLASVRIDDGIAEG
                     GEVSPFYDAMVAKVIVHGRDRADALRRLAAALEDTPLVGLRNNGRYLRDLARHPEFVA
                     GAVTTRRLDDWAAAQAPINLAPVADDEDWAIAAALRSQGPGDAPASGPVALGVASRRV
                     ALACAGVTRALAVAGTRVRVGEREIDVVLHPAGADGWRAVEIDGVRRQRLVVDDGERV
                     HVVRDAAVHQFIEPSPCPQPDAAAAARVARAPVAGVVAQVSVAAGQRVAAGQPLVCVE
                     AMKMEMWLNAAAAGVVRAVNVAARDTVASGAVLVELELDPA"
     gene            complement(7921..8730)
                     /locus_tag="VIN75_17035"
     CDS             complement(7921..8730)
                     /locus_tag="VIN75_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8975926.1"
                     /translation="MEAPAFETLAVRADRGVLHVALNRPDMRNAMSLRMVDELVEVLR
                     AAEASGDARVIVLRGAGGHFCAGADLQDLARARQRELAGDGDAIAAVNARFGALCGAF
                     ARTGIATVAVLEGTVMGGGLGLACVVDVALAGQGAQLRLPETSLGVVPAQVAAFLVER
                     VGASQARRLAVTGGKLDAAQALAIGLVHEVHADGDALDAALAATLAQILRCAPGAIAA
                     TKALMARALHTPPAELIEDAARLFARAARGPEGIEGMTAFLQKRAPAWASR"
     gene            complement(8730..9884)
                     /locus_tag="VIN75_17040"
     CDS             complement(8730..9884)
                     /locus_tag="VIN75_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8975927.1"
                     /translation="MKFTTEHKALSETVKRFVEKELNPFVPKWEADEQYPAHEVFKKL
                     GELGLLGLKYPEEFGGAGLDFSYSMVMAEALGDCLCGGVPMSIGVHTDMCTPALARFG
                     SDELRREFLVPSIAGDVVGCLGVSEPGGGSDVAAVKTTARKDGDDYIIDGSKMWITNG
                     LQADWCCLLANTGDGPAHKNKSLIIVPMNLPGITRQKIHKIGMDSSDTALLFFDGVRV
                     PRRNLIGQEGMGFAFQMMQFQEERLWGAASSLRSLDRLIDLTIEYTRERKAFGKSILD
                     NQVVHFRLAELRTEVAALRALTYQACELYVSGKDVTMLASMAKLKCGRLSREVADSCL
                     QYWGGMGFTRDNPVSQAYRDSRLISIGGGADEVMLGIICKLEGTLPKGAH"
     gene            complement(10007..>10744)
                     /locus_tag="VIN75_17045"
     CDS             complement(10007..>10744)
                     /locus_tag="VIN75_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8975928.1"
                     /translation="PADEREPYDVREVIARLVDGSDFLEFKPTFGADTLCGHARVDGH
                     VVGILGNNGPIQPGGANKAAQFIQLCDQSGTPLVFLQNTTGYMVGSAAERGGAIKHGA
                     KMIQAVANARVPKFTFLIGGSFGAGNYGMCGRGFDPRFIFSWPNARTAVMGGAQAGKV
                     MEIVGAGKLARTGAPVDADALAAMGNQLRDRIERESHVLFGTARLWDDGIVDPRDTRR
                     VLALALALAREAESRTLRPNAFGVARF"
BASE COUNT         1485 a         3898 c         3834 g         1527 t
ORIGIN      
        1 cgcagggcgt cgccgttggc gcgcatgcgc ttgagcatgc tcagcagcgc gcgccattcg
       61 tcgtgcgaga agccggccag gtgcaggttg agcacctcgg ccagcaccgc cgggacgacc
      121 gcggcgacct cgcggccggc atcggtgagc tcgaggttga ccacgcgacg gtcctcgctc
      181 gagcgcacgc gggccaccag gcccttggcc tcgaggcggt cgagcgcgcg ggtcatggcg
      241 cccgggtcgc tctgcagcgt gcgggcaagc tcggccacgg tgccgcattc gccatgggcc
      301 agcttgaaca gcggcaccca ttgggcatgc gtcaggtcgt ggtcgaccag gcggcggtcg
      361 acctggtggg tgatcgacgc aatgacgccc ttgagcacgg cgcccacgct ttcctcctgc
      421 cggtagctgt cggcggagta gaagtcggcc cggaggggat cgttggtgga tgacatggaa
      481 gcgaaatata cttgcttagg cagtcaatgc atcgacaatc attgaatagg ctttaattgc
      541 gtgggggtat accctttgtc acgcgtctgg aagcctcaag taacccgcgc cccgtagact
      601 ggatcgccat gaaatctgct tccgaacgtg ccaaggcggc ttcgccgcgc tcactttccg
      661 ggctactgcc gttcctgcgg ccctaccgcg gccgcatcct ggccgcgctc ctgttcctgg
      721 tggcggcggc catcaccacc ctggctttcc cggtcgtgct gcgcaagctc atcgacggcg
      781 ggctcgggct gtccggcgcc gcctccctgg gcagcggcgc gacctccgcc gaggccaacg
      841 gcgcgcggct gctggcgctg cgcgaccact tcctcgcgct gttcggtgtc ggcgtcgcgc
      901 tcggcgtgtt ctcggcgctg cgcttctatt cggtgacctg gctgggcgaa cgcgtgacgg
      961 ccgacctgcg caacgccgtc taccgccacg tcgtcaagca gagccccgcc ttcttcgaga
     1021 ccaccgccac cggcgaggtg ctgtcgcgcc tgaccaccga cacgacgctg gtgcagcagg
     1081 tcgtcggctc cagcctgtcg atggggctgc gcaacggggt gatgggcctg ggcgcgatgg
     1141 tgatgctgat cgtcaccaac ccgtacgtga tgacgcaggt gctgggcatc ctgctgctgg
     1201 tggtggcgcc gtcgatggcc ttcggccggc gcgtgcgcaa gctcagccgc gccagccagg
     1261 atcgcgtggc cgactccagc gcgatcgccg ccgaggtgct caacgccatc cccgtcgtcc
     1321 agagctacgt gcaggagcac cgcgaggccg cgcgcttcga ggtctccacc gagcatgcgt
     1381 tcgacaccgc ccgcaagcgc acgacggtgc gctcgatgct ggtggcgttc atcatcagcg
     1441 cgacgttcgg cgcgctgctg tggggcctgt accagggcac gcaggcggtc atcgccggcc
     1501 gcatcaccgc gggccacctc ggcgagacgg tggtgtacgt gatgctgctg gtcagcagcg
     1561 tggcggtgct gtcggaggtg tacggcgacc tgctgcgcgc ggcgggcgcc accgagcggc
     1621 tgatggagct gctggccgcc gtctcgcctg tcgccgaccc ggcatcgccg cgcacgctgc
     1681 cgcccaccgg ctcggggtcg tccgtcgagc tgcggcacgt gcagttcaac tatccgtcgc
     1741 ggccgatgca cgcggcgctg gccgactact cgctacgcgt gaggccgggc gagacggtgg
     1801 cgctggtggg ccccagcggc gccggcaaga gcaccgtgtt ccagctgctg ctgcgcttct
     1861 acgacaccgg cagcggcgcc gtcacggtgg acggcgtcga cgtgcgcgag acctcgctgc
     1921 acgagctgcg cgcgcgcatc ggcatcgtgc cgcaggactc caccgtcttc tccgccaacg
     1981 cgatggagaa catccgctac ggccggcccg acgcgagcga cgacgaggtc atcgccgccg
     2041 ccagggccgc cttcgccgac gagttcatcc gccgcctgcc cgagggctac gacaccttcc
     2101 tcggcgagcg cggcatgcgc ctgtcgggcg gccagcgcca gcgcatcgcg atcgcgcgcg
     2161 cgatgctcaa gaacccgccg ctcctgctcc tggacgaggc caccagcgcg ctcgacgccg
     2221 agagcgaacg catggtgcag gccgcgctgg aggccgccat ggccgaccgc acgacgctgg
     2281 tgatcgcgca ccgcctggcg accgtccaga aggccgatcg catcatcgtg atggaacaag
     2341 gtcgcatcat cgaggaagga aatcaccagg aactgatgca gcgcgacggc ctgtacgcga
     2401 aacttgccag aatgcagttc aacaccgagc cgctcgcgcc cgacgacgag acggccgcgt
     2461 ccgaagaagc cgcctgaccc cgccccgatc ccatgagtac cccgccggaa gacatcgacg
     2521 ccctgttcga cctgcctccc cccgctgctc ccgtcctgcc ggcgtcgccg ttgcccggcc
     2581 tgccgccgcc gtccgccgat tcccgcaagg agcagcgcgt caaggtctcg tggcccgcgc
     2641 gcgtgcagct gccgggcggc cgcgtcatcg agctgcgcgt gcgcgacttg tgcgagagcg
     2701 gcgtgggcct gctcaccgag caccacatcc cgcccgccac ggtgctgaac ttcgcggtcg
     2761 ccgtgccggg gctgaacgag ggcggcagga tgacgccggt gtcgggcacc atcaagacga
     2821 cctacgtcgt cgtcaagggc gtggacctgt tctgcggcgg cacctgggtg cagttgccgc
     2881 cggacggacg cgacctgctg gacaagtgga tccgcaagct gcgcaggtag ggcgggtcag
     2941 cgcagcgtaa cccgccctgc gtcccgcttc agggcgcgta cagcgcggcg cagtggccgg
     3001 gctgcgacgg gctggccgag accgcgccgc ccttggggat gcactggctc gccggcagcg
     3061 cggcgtgcgt ctcgacgtgc gccaccatca ggtcgaaggc cgcgtgcgcg taaggctgga
     3121 tgagcgccag ctgcgggaag ctgtcctggt aggtctcgat gtggttgccg ttctgcacct
     3181 cgtacaggcg gtaggcggcg tcgcgctcgt cgtggcgcgg gccccagccg tcgtggtcgc
     3241 ggtcgcgcga ctgcgcggcg gccaccttgc gggcgtaggc gcgcgcgtgg tggtcgatcg
     3301 gcagcagcgc gtccatcgtg ccggccacgg tgaccagcgg acgctggatg cggccggtgg
     3361 tcgcgaacga cgccacgttg gcgccgacgt cggaggccga cagccgcgcc acgtagttgt
     3421 agttgccggt gcccgaccca taggtgtcgt aggtcgggtc gaggcgcttc tgccactggc
     3481 actgcgtcac ttcccagaac tgcgcccagt agctggtcca cagcgtcgac gtcgggctgg
     3541 cgacgatgtc gggcggatag ccggccgcgc gcatgttctt cgcggcggtg ctgttcgggt
     3601 cgaggcccga ggccgcgtag tcgttgtagt tgaggatggc cgccggcagg tcggtgagca
     3661 ggttgggcgc gtgctcgtcg acgaaggtgc cttcccagtc gacgccgccg tcgaacagct
     3721 cgggcgccgt ctccatcgcg cgccgcacct ggtagccgcc gttggacgtg cccaccgcgt
     3781 acgtgaagcg cggcgggcgg ccgtagttgg acgccacgcc ctggcgcgcg agctgcgcgg
     3841 ccttgatcat gtactcctgc cagcgcgcga actgctggtc ggggccgttg tcgtagaagt
     3901 cgacgaaggt cgtcgacacc ggattgaggc ggcagcccag cggatcggcc gccgtcgtca
     3961 gctgcaggtt cagcacgccc ttgttctgcg acgcataggc gtagcccttc tggacgacgt
     4021 agtcgctcca ggcgaagtcg ccgttgaact cgctgcgcgt gcccgacgcg ccggccacga
     4081 ccagccggcc gttccagtcc ttgggcaggc gcaggaggaa gcgcgcctgg cccgtcgggt
     4141 cggacgcgat gcggccgttg agctggatgc ccggcaccac cttcgtgatg gggtcgcgct
     4201 tggccgcgct cggcgagatc gtcgcgcggt cggtctgcgg cgtgaacgcg aacgccggca
     4261 gcgacgcgat tgcgttgttg gccggcgtgg tgtccgggtt ggtggtggtg aggtccgaac
     4321 tgtcgacgca ggtcgcatcg gccagctgcg aaccgaactg cttgagcagg tggtcgcagg
     4381 ccgtggacgc gctggcggcc aggggaacga aacaggcgag cgccatcaag ggcgcccgac
     4441 ggcgaatcac gatcgtcatc ggcaagtctc cttggggttt tcgaagcccg cgaagactac
     4501 ttccgcgtca tgcgccgcgc aattggtcct tggtgctacc cgtcacatca cctaagtgga
     4561 atcccggacg aacggcgcgc ccatcaggag tgctgcggcg gccccgactt gcgcccgtat
     4621 gccgccgtgc ccgtggcgcc cagcacgccg cccagcacca gcaggtagca caacgccagc
     4681 gtgcccgccg tgccctggat ggccagcagg ccggcgccgc cggccaccag cgcgaccgcc
     4741 gccagcagtg ccagcagcag cagcgcgctc gcgacgacgc gcgtgggcgt gccgcgcagc
     4801 gggtcgacgt cgctgacgac gagcaggaac cagccgacca gcgtcagcaa cgggaacagg
     4861 atccacagcg acggcccggc cgccgccagg ccgaaggccg cggcgccgag cagcgcgccg
     4921 gccaggatgc ccaatgcggc cagcaggccg cccgcgatcg cgatcatgac gttcgctccg
     4981 agtccagatg ccgccaggat agtccaacgg cggccacccg cccagcgcgg gtgcgtcgcg
     5041 tcagaccctg gccaatccca gcagttcccg cgcccgcgcc ggcgaggcga tgcggcgccc
     5101 ggcgcgctcg gcgcaccgcg ccagcgccgc gatcatcggc ccgttgccgc tcgcgcgttc
     5161 gccaccgggc aggtagaagc tgtcctccag gccggtgcgc agcatgccgc cgagctcggc
     5221 ggtcttctgg tggacgggcc agatctcctc gcggccgatc agcgtcgtct gccacaccgc
     5281 gccgggcgcg atccagcgcg gcagcagcgc gagcaggtcg gcgtcgcaag gcatgcccga
     5341 ggccacgccc atcacgaggt tcacctcggg ggtgcccatg gacggcttga ccatgcccgc
     5401 cttcacgaac atcgccaccg agcgcacgat gccgacgtcg aagcactcga actccgggtg
     5461 cgcgcccgcg gcctccatcg cgtcgaggaa ctgctgcacc ttggcgaccg ggttgtcgaa
     5521 caccatcggc ggccaggccc agcggccgtc cgagcgcagc ttcaggtagt tgaggctgcc
     5581 ggcgttgcag gcggcgatct cgggccggac gcgctcgatg caggccagcg ggccgctgat
     5641 gtccttgccg atcacgccgg tcgtcaggtt gatgatcact ccgggacagg ccgcgcggat
     5701 cgcgtcgcag acctcggccg cgaccgccgg atcccacgac ggcaggtgcc cttgcccggg
     5761 ctcctggcgg cggacgtgca cgtgcatgat ggacgcgccc gcgccgaacg cgtcgcgcgc
     5821 ctcggccgcc atctgggccg cggtgacggg caccgggtgc tgggcggggt cggtgagcac
     5881 gccggtcagc gcgcaggtca ggatcgcatc gtccatcgca tgtccttcaa gcgggatcca
     5941 gctcgagctc gacgagcacc gcgcccgacg ccaccgtgtc gcgcgcggcg acgttgaccg
     6001 cgcgcaccac gcccgccgcc gcggcgttga gccacatctc catcttcatc gcctcgacgc
     6061 acaccagcgg ctgcccggcc gcgacgcgct ggccggccgc gaccgacacc tgcgccacga
     6121 cgcccgccac cggcgcccgc gccacgcgcg cggccgcggc cgcgtcgggc tgcggacacg
     6181 gtgaaggctc gatgaactga tgcacggccg cgtcgcgcac gacgtgcacg cgctcgccgt
     6241 cgtcgacgac caggcgctgg cgccgcacgc cgtcgatctc gaccgcgcgc cagccgtcgg
     6301 cgccggccgg gtgcagcacg acgtcgatct cgcgctcgcc gacgcgcacc cgcgtgcccg
     6361 cgaccgccag cgcacgcgtc acgcccgcgc acgcgagcgc gacgcggcgc gaggccacgc
     6421 ccagcgcgac cggcccgctg gccggcgcgt cgccgggccc ctgcgagcgc agcgcggccg
     6481 cgatggccca gtcctcgtcg tcggcgaccg gcgcgaggtt gatcggcgcc tgcgcggcgg
     6541 cccagtcgtc caggcgccgg gtcgtgacgg cgccggcgac gaactcggga tggcgcgcca
     6601 ggtcgcgcag gtagcggccg ttgttgcgca agcccacgag cggcgtgtcc tccagcgcgg
     6661 cggcgagccg tcgcagcgcg tccgcgcggt cgcggccgtg cacgatgacc ttggccacca
     6721 tcgcgtcgta gaacggcgag acctcgccgc cctcggcgat gccgtcgtcg atgcgcacgc
     6781 tggcgagcgt ggccacggga acgccctgcg tgcccgcgtc ggcgcggcct gcgcgcagcg
     6841 cccgcgccgg gcgccagtac gcgatgcggc ccgtctgcgg cgcgaagccg tcgtagggat
     6901 cctcggcgta cagccgcgcc tggacggcat ggcccgacca gcggatctgg tcctggcgca
     6961 gcggcagcgg ctcgcccgcg gccacgcgca gctgccattc gacgaggtcc aggccggtca
     7021 cgcattccgt caccgggtgc tcgacctgca gccgcgtgtt catctccagg aagaagtggc
     7081 ggcgctgctc gtcgacgatg aattccaccg tgcccgcgcc gacgtagccg accgcacgcg
     7141 cggcggccac cgcgtcgcgg cccatcgcct cgcgcatggc ggcgtcgacg tacggcgacg
     7201 gcgactcctc gatcaccttc tggcgccggc gctgcgcgct gcagtcgcgc tcgcccaggt
     7261 gcacgacatt gccgtgcgca tcggcgaaca cctggatctc gatgtggcgg ccctcggcga
     7321 cgaggcgctc gagcatcagc gtgccgtcgc cgaaggccga cgtcgcttcg cgccgggcgc
     7381 ccgcgagtgc atcggccaat ccggcctgtg cgtgcacgag gcgcatgccg cgcccgccgc
     7441 cgccggccac ggccttgacc agcagcggat agcccagcgc gttcgcctgc gcgacgagcg
     7501 ccgggtcgga ctggtcgtcg ccgagatagc ccggcacgca cggcacgcct gcggcgagca
     7561 tgcggcgctt ggcgccggcc ttgtcgccca tcgcgctgat cgccgatgcc ggcggcccga
     7621 tgaagacgag cccggcgtcc acgcaggcct gcgcgaagtc ggcgcgctcg gacaggaagc
     7681 cgtagcccgg atggatcgca tcggcgccgg tgcgccttgc ggcggccagg atcgcggcta
     7741 tgcgcagata cgaatcggcc gcggcctggc caccgagcgc cacggcctcg tcggcgagcg
     7801 cgacgtgcgg cgagccggcg tcgggctcgc tgaagacggc gacggtgcgg tagccgagcg
     7861 agcgcgcggt gcggatcacg cggcaggcga tctcgccgcg attggcgacc aggatcctgt
     7921 tcatcgcgaa gcccacgccg gcgcccgctt ctgcaggaac gccgtcatgc cctcgatgcc
     7981 ttccggcccc cgcgcggccc gcgcgaacag gcgcgcggcg tcctcgatca gttcggccgg
     8041 cggcgtgtgc agcgcgcgcg ccatcaaggc cttggtcgcg gcgatcgcgc cgggcgcgca
     8101 gcgcaggatc tgcgcgagcg tcgcggccag cgcggcgtcc agcgcgtcgc cgtccgcatg
     8161 cacttcgtgc acgagaccga tcgccagcgc ctgcgccgcg tcgagcttgc cgccggtcac
     8221 cgccaggcgc cgcgcctgcg acgcgccgac gcgctcgacc aggaacgcgg cgacctgcgc
     8281 cggcacgacg cccagcgagg tctcgggcag gcgcagctgc gcgccctgcc cggccagcgc
     8341 cacgtcgacc acgcacgcga gcccgaggcc gccgcccatc accgtgccct ccagcaccgc
     8401 gaccgtcgcg atgccggtgc gcgcgaacgc gccgcacagc gcgccgaagc gggcgttgac
     8461 ggcggcgatg gcgtcgccgt cgccggcgag ttcgcgctgt cgcgcccggg ccaggtcctg
     8521 caggtcggcg ccggcgcaga agtggccgcc cgcgccacgc agcacgatga cgcgcgcgtc
     8581 gcccgacgcc tccgcggcgc gcagcacctc gaccagctcg tcgaccatgc gcagcgacat
     8641 cgcgttgcgc atgtcgggcc ggttcagcgc gacgtgcagg acgccgcggt ccgcacggac
     8701 cgccagcgtc tcgaaggccg gggcctccat caatgcgctc ccttcggcag cgtgccctcg
     8761 agcttgcaga tgatgcccag catcacctcg tcggcgccgc cgccgatgga gatcaggcgc
     8821 gagtcgcgat aggcctgcga gacggggttg tcgcgcgtga agcccatgcc gccccagtat
     8881 tgcaggcagc tgtcggccac ctcgcggctg aggcgtccgc acttgagctt ggccatcgac
     8941 gcgagcatcg tcacgtcctt gcccgacacg tacagctcgc aggcctggta cgtgagcgcg
     9001 cgcagcgcgg ccacttccgt gcgcagctcg gcgaggcgga agtgcaccac ctggttgtcg
     9061 aggatgctct tgccgaaggc cttgcgttcg cgcgtgtact cgatcgtcag gtcgatgagg
     9121 cggtcgaggc tgcgcaacga cgaggcggcg ccccacagcc gttcctcctg gaactgcatc
     9181 atctggaagg cgaagcccat gccctcctgg ccgatgaggt tgcgccgcgg cacgcgcacg
     9241 ccgtcgaaga acagcagcgc ggtgtcggac gagtccatgc cgatcttgtg gatcttctgc
     9301 ctggtgatcc ccggcaggtt catcggcacg atgatgaggc tcttgttctt gtgcgccggg
     9361 ccgtcgccgg tgttggccag caggcagcac cagtcggcct gcaggccgtt ggtgatccac
     9421 atcttgctgc cgtcgatgat gtagtcgtcg ccgtccttgc gcgccgtcgt cttcacggcc
     9481 gcgacatcgg agccgccgcc gggctcgctc acgcccaggc agccgacgac gtcgccggcg
     9541 atggacggca cgaggaactc gcggcgcagc tcgtccgagc cgaagcgcgc cagcgcgggc
     9601 gtgcacatgt cggtgtgcac gccgatggac atcggcacgc cgccgcacag gcagtcgccg
     9661 agcgcctcgg ccatcaccat cgagtagctg aagtccagcc ccgcgccgcc gaactcctcg
     9721 gggtacttca ggcccagcag gccgagctcg cccagcttct tgaagacctc gtgcgccggg
     9781 tactgctcgt cggcctccca cttcggcacg aacgggttca gctccttctc gacgaagcgc
     9841 ttgacggtct cggacagggc cttgtgttcg gtcgtgaact tcattgcgtg agctctgatg
     9901 tggattcgaa gaagacggtg gtcgccggcg agcgcccggc cgctccggag cgagccggcg
     9961 tgagcccctc gggggcggcg agcggagcga gccgggcggt catggcttag aagcgggcga
    10021 cgccgaacgc attcgggcgc agcgtgcggg actcggcctc gcgcgccagc gccagcgcga
    10081 gcgccagcac gcggcgcgtg tcgcgcgggt cgacgatgcc gtcgtcccac agccgcgcgg
    10141 tgccgaacag cacgtgcgac tcgcgctcga tgcggtcgcg cagctggttg cccatcgcgg
    10201 cgagcgcgtc ggcgtcgacc ggcgcgccgg tgcgcgcgag cttgcccgcg ccgacgatct
    10261 ccatcacctt gccggcctgc gcgccgccca tcacggcggt gcgcgcgttc ggccagctga
    10321 agatgaagcg cggatcgaag ccgcggccgc acatgccgta gttgccggcg ccgaacgagc
    10381 cgccgatcag gaacgtgaac ttgggcacgc gggcattggc caccgcctgg atcatcttgg
    10441 cgccgtgctt gatcgcgccg ccgcgctcgg cggccgagcc caccatgtag ccggtggtgt
    10501 tctgcaggaa gaccagcggc gtgcccgact ggtcgcacag ctggatgaac tgcgccgcct
    10561 tgttggcgcc gccgggctgg atcggcccgt tgttgccgag gatgcccacg acgtggccgt
    10621 cgacgcgcgc gtggccgcac agcgtgtcgg cgccgaaggt gggcttgaac tcgaggaagt
    10681 cggagccgtc gaccagtcgc gcgatcacct cgcgcacgtc gtagggctcg cgctcgtcgg
    10741 ccgg
//
LOCUS       DATCRO010000623         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_197973, whole genome shotgun sequence.
ACCESSION   DATCRO010000623 DATCRO010000000
VERSION     DATCRO010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197973"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIN75_17050"
     CDS             <1..479
                     /locus_tag="VIN75_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975929.1"
                     /translation="LVLIMTTSGSRRLPGPTREARRVLLARPADSRPETLVAHAVRVF
                     TVIGSPAFRLDPADLRTRAEASIARASRPAGTARQLLAIAADGDRTPLLARIQAPTQV
                     IHGVADPLVPVASGHDLARRIPGALADFIDGMGHDLPPQLLGRIARGIAGNARRAT"
     gene            complement(595..3036)
                     /locus_tag="VIN75_17055"
     CDS             complement(595..3036)
                     /locus_tag="VIN75_17055"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEY8975930.1"
                     /translation="MKKQGQAKSRQAPSGRADKLKVLSALTASVFALSAQAATTEAWV
                     ATSTKAHDPRAAVHVAPLKAGEQVDIVVSLKLRNKAELDALTSKLMTGAAGSRPLTSA
                     EFMAKHAPTEADAQAVVAYLRAQGFTNIEVAPNRLLVSATGTAQAIRSAFKADLHEYN
                     VNGRRAYANVTDAMIPQHLAASVLGVVGLQNVHMMHTHAQRMNAHPEAATPQAVTGVS
                     IPNFASIYGASSLPSATTGTIGIITAGSLTQTITDLKNFASSAGYPVPNVTKTVVGTA
                     GTSTSGTDEWNMDSQSSLAAAGGSISQMILYNVNSLSDSALSQGYNKAVTDNKARAIN
                     VSLGGCESDEGSVEASQDAIFQSAVAQGQMFSVSSGDSGAYECGATAGKAQSYPAVSP
                     YVMAIGGTTLSSSGGTWQGETVWSCSSASTCQQSSSGGAGGGPSLTENAPSWQTAAGV
                     LGTSTKRGVPDISFDASPSSGALVLVNGSNAQIGGTSLAAPLWAGFWTRIQAAHGNSL
                     PFPASAIYSGAAANPQWFHDVTSGNQGYAAGTGWDYASGYGSVIISALSANITNTPPP
                     PPPAGPTANFTDTVSGLTVSFVDTSTDTGGTITSRSWTFGDGGTSTAQNPTHTYAAAG
                     NYSVTETVTDSNSKTSSKTASVSVSVTPPPPPPPANLVLNPGFESGTTSWTATSGVGC
                     TNSTCSGETAHSGTGFAWLDGYGSAHTDSVSQSITIPAGKTSATLTFYLHIDTQETGS
                     TAYDKLTVSATPSGGSATTLATYSNVNAASGYVLRTISLNSFIGKTITLKFSGTEDSS
                     LATSFVLDDVSVAVQ"
     gene            3056..3283
                     /locus_tag="VIN75_17060"
     CDS             3056..3283
                     /locus_tag="VIN75_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975931.1"
                     /translation="MGFTQIDGVIFLLCERQNDFVAVARGSIAITDWVIQFGGRSSMQ
                     ALILCRANICGIGSNPEGYVVHTLHLDRAFG"
     gene            complement(3596..>4338)
                     /locus_tag="VIN75_17065"
     CDS             complement(3596..>4338)
                     /locus_tag="VIN75_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY8975932.1"
                     /translation="YHRLNARDGVTTELAKATVRRSATTIAALMVHLGDADALLCGLV
                     GRFDRHFGHLREVIRQRSDTHGFATVNALLLDKHTLFIADTSVNDDPTAEQLADIAAM
                     AVEEVRSFGLVPQVAFLSHSMFGSSTRPSARKMRAARDLFVQRMPDVSADGELQGDAA
                     LSPELREALLPDSTLQGAANVLICPNLDAANILFNVLKMTGGHGVTIGPILLGADAPV
                     HVLTPSSTVRRIVNMTALAVARAGSAAS"
BASE COUNT          636 a         1368 c         1593 g          741 t
ORIGIN      
        1 gcctggtgct gatcatgacc accagcggat cgcgccgcct gccggggccg acgcgcgagg
       61 cgcgccgcgt gctgctggcg cggcccgccg acagccggcc cgagacgctc gtcgcgcacg
      121 ccgtgcgcgt gttcaccgtc atcggcagtc ctgcgttccg gctcgatccg gccgacctgc
      181 gcacgcgtgc cgaggcgagc atcgcgcgcg cctcgcgccc ggccggcacc gcgcggcagc
      241 tgctggccat cgcggccgac ggcgaccgca cgccgctgct ggcccgcatc caggcgccca
      301 cgcaggtgat ccacggcgtg gccgacccgc tggtcccggt ggccagcggc cacgacctgg
      361 cgcgccgcat tcctggcgcg ctcgccgact tcatcgacgg catgggccac gacctgccgc
      421 cgcagctgct gggccgcatc gcccggggca tcgccggcaa cgcacgccgc gcaacctgaa
      481 cgccggcttg tcatctgccg gcttgcccga tgaaaaaggg ccgatcggtc gcccgatcgg
      541 cccttggcct tgtcgaaggg cgcgacgcgg ggagtgcgtc gcgccgtccg tggcttactg
      601 cacggccacc gagacgtcgt ccaggacgaa cgaggtcgcc agcgacgagt cttccgtgcc
      661 cgagaacttc agcgtgatcg tcttgccgat gaagctgttc aggctgatcg tgcgcaggac
      721 gtagcccgaa gccgcgttga cgttggagta cgtcgcgagc gtcgtggccg agccaccgct
      781 cggagtggcc gagaccgtca gcttgtcgta cgcggtcgag cccgtctcct gcgtgtcgat
      841 gtgcaggtag aaggtcaggg tcgcggacgt cttgccggcc gggatcgtga tcgactgcga
      901 gacgctgtcg gtgtgggccg agccgtagcc gtcgagccag gcgaagcccg tgccgctgtg
      961 cgccgtctcg cccgagcagg tcgagttcgt gcagccgacg ccggacgtgg ccgtccagct
     1021 ggtggtgccc gattcgaagc ccgggttcag gaccaggttg gccggcggcg gcgggggcgg
     1081 cggggtgacc gacaccgaga ccgaggccgt cttcgacgac gttttgctgt tgctgtccgt
     1141 caccgtttcc gtcaccgagt agttgccggc ggcagcgtac gtgtgggtcg ggttctgcgc
     1201 ggtcgaggtg ccgccgtcgc cgaaggtcca cgaacgcgag gtgatcgtgc cacccgtgtc
     1261 ggtcgaggtg tcgacgaacg acaccgtcag gcccgagacc gtgtcggtga agttggcggt
     1321 cgggccggcc ggcggcggcg gcggcggcgt gttggtgatg ttggcggaca gggcgctgat
     1381 gatcaccgag ccgtagcccg acgcgtagtc ccagccggtg ccggcggcgt agccctggtt
     1441 gcccgaggtc acgtcatgga accattgcgg gttcgcggcg gcgcccgagt agatcgccga
     1501 ggccgggaag ggcagcgagt tgccgtgcgc ggcctggata cgcgtccaga agccggccca
     1561 cagcggagcg gccagcgagg tgccgccgat ctgcgcgttg ctgccgttga ccagcacgag
     1621 ggcgccggag ctgggcgagg cgtcgaacga gatgtccggc acgccgcgct tggtggaggt
     1681 gccgagcacg cccgcggcgg tctgccagct gggggcgttt tccgtcagcg aggggccgcc
     1741 gccggcgccg ccggaggacg actgctggca ggtggaggcg ctggagcacg accacaccgt
     1801 ctcgccctgc caggtgccgc cggagctcga cagcgtcgtg ccgccgatgg ccatgacgta
     1861 cggcgagacc gcggggtagc tctgcgcctt gccggcggtg gcgccgcact cgtacgcgcc
     1921 ggagtcgccc gacgagaccg agaacatctg gccttgcgcg acggccgact ggaagatggc
     1981 gtcctgggaa gcctcgaccg agccttcgtc gctttcgcaa ccgcccagcg acacgttgat
     2041 cgcgcgggcc ttgttgtcgg tgacggcctt gttgtagccc tgggacagcg cggaatcgct
     2101 cagcgagttc acgttgtaca ggatcatctg cgagatcgag ccgccggcgg ccgccagcga
     2161 gctctgcgag tccatgttcc actcgtccgt gcccgaggtg ctggtgccgg cggtgccgac
     2221 gaccgtcttc gtgacgttgg ggaccgggta tccggcgctc gaagcgaagt tcttcaggtc
     2281 ggtgatggtc tgggtcagcg aacccgcggt gatgatgccg atcgtgccgg tggtggcgct
     2341 cggcaggctc gacgcgccgt agatggacgc gaagttgggg atgctgacgc cggtcacggc
     2401 ttgcggcgtg gccgcctccg gatgcgcgtt catgcgctgg gcgtgcgtgt gcatcatgtg
     2461 cacgttctgc aggcccacga cgccgaggac cgaggcggcc aggtgctgcg ggatcatcgc
     2521 gtcggtgacg ttcgcgtagg cgcggcggcc gttgacgttg tactcgtgca ggtcggcctt
     2581 gaacgccgag cggatggcct gggcggtgcc cgtggcgctg accagcaggc ggttgggggc
     2641 gacctcgatg ttggtgaagc cctgcgcgcg caggtaggcg acgacggcct gggcgtcagc
     2701 ctcggtcggc gcgtgcttgg ccatgaactc ggcggacgtc agcggacgcg agccggcggc
     2761 gccggtcatc agcttggagg tcagcgcgtc gagctcggcc ttgttgcgca gcttcagcga
     2821 gacgacgatg tcgacctgct cgccggcctt cagcggagcc acgtggacgg cggcgcgggg
     2881 atcgtgggcc ttggtgctcg tggcgaccca tgcttcggtg gtggcggcct gggcagacag
     2941 cgcgaacacg ctggccgtca gggccgacag gaccttgagc ttgtcagccc gccccgaagg
     3001 agcctgccgg ctcttggctt gtccttgttt cttcatggaa ctcttctcct aatcgatggg
     3061 attcacgcaa attgatggcg tgattttttt gctatgtgag cggcaaaacg attttgtcgc
     3121 tgtcgcccgt ggaagcatcg cgatcaccga ttgggttatc cagttcgggg ggaggtcgtc
     3181 gatgcaggcg ctgattctgt gccgcgcgaa tatctgcgga atcgggtcga accccgaggg
     3241 gtatgtggtg cataccctgc acctcgaccg tgcattcgga tgaagttggt gcgcacaccc
     3301 aggtgttctt cagcctgggg acagatgtgt ttccgagggt gcgaaattgt tgccgagggg
     3361 attaacgaag ggttaattgc acatggcact tcgaatactt gagcattaaa tcaattcggt
     3421 ctgggagttc gggaaatccc tgttcgggat taggcgaggt tccccaatcc aaataaacaa
     3481 ataacctttg acgtacaacg aaaaacgcgg ccgtgaaggc cgcgtggata cccgattcaa
     3541 ggtattttgc gaatcgcgat acgattttcg gggtatatac cgacaggggt ttctctcagc
     3601 tggcggcact tccggcgcgc gccacggcca gcgccgtcat gttgacgatg cggcgcacgg
     3661 tcgacgacgg cgtcagcaca tgcaccggcg cgtcggcgcc cagcaggatg gggccgatcg
     3721 tcacgccgtg gccgcccgtc atcttgagca cgttgaacag gatgttggcg gcgtccaggt
     3781 tgggacagat cagcacgttg gcagcgccct gcagcgtcga atcgggcagc agggcctcgc
     3841 gcagctcggg gctgagcgcg gcatcgccct gaagctcgcc gtcggccgag acgtcgggca
     3901 tgcgctgcac gaacaggtcg cgcgcggcgc gcatcttccg ggcggacggc cgcgtgctgc
     3961 tgccgaacat cgagtgcgac aggaacgcca cctgcggcac caggccgaag ctgcgcacct
     4021 cctcgaccgc catcgcggcg atgtcggcca gctgttcggc cgtcgggtcg tcgttgacgg
     4081 aggtgtcggc gatgaacagg gtgtgcttgt ccagcagcag cgcattgacg gtggcaaagc
     4141 cgtgggtgtc gctgcgctgg cggatcacct cgcgcaggtg cccgaagtgg cggtcgaagc
     4201 ggcccaccag gccgcacagc agggcatcgg cgtcgcccag gtgcaccatc agcgccgcga
     4261 tcgtggtggc ggagcgccgc acggtggcct tggccaactc ggtggtgacg ccgtcgcgcg
     4321 cattgaggcg gtggtacg
//
LOCUS       DATCRO010000624         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_974494, whole genome shotgun sequence.
ACCESSION   DATCRO010000624 DATCRO010000000
VERSION     DATCRO010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974494"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /gene="grxC"
                     /locus_tag="VIN75_17070"
     CDS             <1..243
                     /gene="grxC"
                     /locus_tag="VIN75_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY8975933.1"
                     /translation="MFTTQVCPYCIRAKQLLKQRGVDAIEEIRVDLDPGQRATMIAET
                     GRRTVPQIFIGETWVGGCDDLYALDQKGGLVPLLNG"
     gene            344..802
                     /gene="secB"
                     /locus_tag="VIN75_17075"
     CDS             344..802
                     /gene="secB"
                     /locus_tag="VIN75_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY8975934.1"
                     /translation="MAEQDQSPVFQIQRIYLKDLSLEQPNSPQVLLEQTQPQVDIQLS
                     MAAEAIAQGLFEVTVTATVTTKVGDKTLFLVEAKQAGIFEIRNVPAEQQQGILGVVCP
                     QMLYPYLRAIVSDVCTRAGFPPILLAEVNFQAMFEAQQQAALAQANGAPN"
     gene            1012..1995
                     /locus_tag="VIN75_17080"
     CDS             1012..1995
                     /locus_tag="VIN75_17080"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8975935.1"
                     /translation="MRISVIGAGAWGTALAIAAARRHRVLLWARDAAQARQMAARRRN
                     ERYLPHAAWPDQLAVTSDHAAALAHAEGGLVVLATPMAALRERLAALAPASCVFWLCK
                     GVESATGSLAHQIAAGVLPNASVGVLSGPSFAQEVAGGSPTALVAASSDPALCDLAVN
                     AFHSESLRIYRSTDVVGVEVGGAVKNVLAIATGIADGLNLGLNARAALITRGLAEMTR
                     LGLALGAQHETFMGLSGLGDLVLTATGDLSRNRKVGMLLAQGLSLDSILEKLGHVAEG
                     VYCAETVTRLAHDHGVAMPIADMVSAVIHRRIAARDAVASLMRRDSRSEGL"
     gene            complement(2170..2634)
                     /locus_tag="VIN75_17085"
     CDS             complement(2170..2634)
                     /locus_tag="VIN75_17085"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY8975936.1"
                     /translation="MFRIVLVHPEIPPNTGNVIRLAANTGCELHLIEPLGFSMEDKLL
                     RRAGLDYHEFADVRRHARWEDYAATVDASRCFAFSTRGTGTLASVDWRAGDSLVFGSE
                     SAGLPPALREIFPPERLVRLPLLPGQRSLNLSNAVAVAVFEAWRVNGYAGGL"
     gene            complement(2645..>2863)
                     /locus_tag="VIN75_17090"
     CDS             complement(2645..>2863)
                     /locus_tag="VIN75_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY8975937.1"
                     /translation="GHQLGLQRGARQANLKGAFVAAPHHAAWLRGARVALVDDVLTTG
                     ATARAATRALRAAGAREVQVWTVARTPK"
BASE COUNT          416 a         1027 c         1005 g          415 t
ORIGIN      
        1 atgttcacca cccaggtctg cccgtactgc atccgcgcga agcagttgct caagcagcgc
       61 ggggtcgatg ccatcgagga gatccgcgtc gacctcgacc ccggccaacg ggcgacgatg
      121 atcgccgaga ccggccgccg cacggtgccg cagatcttca tcggcgagac gtgggtgggc
      181 ggctgcgacg acctctacgc gctcgaccag aagggcggcc tggtgcccct gttgaacggc
      241 tgagggttca cccctccgac gcggccccgg tgggctgcga cataatgccg gatgcagccc
      301 gcaccgtccc ggtttgcggg cttcgttttt ttcgagacac gccatggctg aacaagacca
      361 atcccccgtt ttccagatcc agcgcatcta tctgaaggat ctgtcgctcg agcaaccgaa
      421 ctcgccgcag gtcctgctgg agcagacgca gccgcaggtc gacatccagc tgtcgatggc
      481 ggccgaggcg atcgcgcagg gtctgttcga ggtcacggtc accgccaccg tcaccaccaa
      541 ggtcggcgac aagacgctgt tcctggtcga ggccaagcag gccggcatct tcgagatccg
      601 caacgtgccc gccgagcagc agcaaggcat cctgggcgtc gtctgcccgc agatgctgta
      661 cccgtacctg cgcgccatcg tgtccgacgt ctgcacgcgc gcgggcttcc cgccgatcct
      721 gctggccgaa gtcaacttcc aggccatgtt cgaagcgcaa cagcaggccg cgctggccca
      781 ggccaacggc gcgccgaact gacgaatccg tgcccgaacc cgacacgccc gctcgttccg
      841 ccagggcgcg cccgctctgc gttgcaaacg ctcgccgtgg caaccagcac gactcgcatc
      901 ggcgccttga tcggacaggc cctgacgggc tgctcggacg cgtcggatcc gaccacggat
      961 tcggctgggt cgtcgtgacg acccttcgcc atggcatctg atcctcgccc gatgcgcatc
     1021 tcggtcatcg gggccggtgc gtggggcacc gcgctcgcca tcgccgcggc gcgccgccac
     1081 cgcgtgctgc tgtgggcgcg cgacgccgcg caggcccggc agatggcggc caggcgccgc
     1141 aacgaacgct acctgccgca cgcggcgtgg cccgaccagc tcgcggtcac gtccgaccac
     1201 gcggccgcgc tcgcgcacgc cgaaggcggc ctggtcgtgc tcgcgacgcc gatggccgcg
     1261 ctgcgcgagc ggctggccgc gctggcgccc gccagctgcg tgttctggct gtgcaagggc
     1321 gtggagtcgg ccaccggctc gctggcgcac cagatcgcgg ccggcgtgct gccgaacgcg
     1381 tcggtgggcg tgctctccgg ccccagcttt gcgcaggaag tggcgggcgg cagcccgacg
     1441 gcgctcgtcg cggccagcag cgatcccgcg ctgtgcgatc tcgcggtcaa cgcgttccac
     1501 agcgaatcgc tgcgcatcta ccgctcgacc gacgtggtcg gcgtggaggt gggcggcgcc
     1561 gtcaagaacg tgctggcgat cgccaccggc atcgccgacg gcctgaacct gggcctgaac
     1621 gcgcgcgccg cactgatcac gcgtggcctg gcggagatga cgcgcctggg cctggcgctc
     1681 ggcgcccagc acgagacgtt catggggctg tccggcctgg gcgacctcgt gctcaccgcc
     1741 accggcgacc tgtcgcgcaa ccgcaaggtc ggcatgctgc tggcccaggg cctgtcgctg
     1801 gacagcatcc tcgagaagct cggccacgtc gccgagggcg tgtactgcgc cgagaccgtc
     1861 acgcggctcg cgcacgacca cggcgtcgcg atgccgatcg ccgacatggt gtcggccgtg
     1921 atccaccgcc gcatcgccgc gcgcgatgcg gtggcgtcgc tgatgcggcg cgattcgcgc
     1981 agcgaaggtc tctgatttcg cggtgtcagg tcttgccttt gcgcggcaac ccgccggctt
     2041 cggggtcagg tcttgaaagt tgcgcgtgga tgcgctcgcc catcgccgcc atgccatcgc
     2101 gcaactttca agacctgacc ccacggtgag gcgaccgagc gcgcaaaggc aagacctgac
     2161 accgcctgat cagaggccgc ccgcgtagcc gttgacacgc caggcctcga acaccgccac
     2221 ggccacggcg ttgctgaggt tgaggctgcg ctggccggga agcagcggca ggcgaacgag
     2281 gcgttcaggc ggaaagatct cgcgcagggc cggcggcagg ccggccgatt cgctgccgaa
     2341 gaccaggctg tcgccggcgc gccagtcgac gctggccagc gtgccggtgc cgcgcgtcga
     2401 gaacgcgaag cagcgcgacg catcgacggt ggcggcgtag tcctcccagc gcgcgtggcg
     2461 gcggacgtcg gcgaactcgt ggtagtcgag gccggcgcgt cgcagcagct tgtcctccat
     2521 cgagaagccc agcggctcga tcaggtgcag ctcgcagccg gtgttggcgg ccaggcggat
     2581 gacgttgccg gtgttgggcg ggatctcggg gtggacgagg acgatgcgga acatcagccc
     2641 attttcactt gggcgtgcgc gccaccgtcc acacctgcac ctcgcgcgcc cccgcggcgc
     2701 gcagcgcgcg cgtggccgcc cgcgcggtgg cgcccgtggt gagcacgtcg tcgaccagcg
     2761 cgacgcgcgc gccgcgcagc caggccgcgt ggtgcggcgc ggcgacgaag gcgcccttca
     2821 ggttggcctg gcgcgcgccg cgctgcaggc ccagctggtg gcc
//
LOCUS       DATCRO010000625         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_929365, whole genome shotgun sequence.
ACCESSION   DATCRO010000625 DATCRO010000000
VERSION     DATCRO010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929365"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /locus_tag="VIN75_17095"
     CDS             <1..2259
                     /locus_tag="VIN75_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975938.1"
                     /translation="TPTIPRADGTDPSPPVPDDPDFRIVLETLVDAYRPILEEDLKRA
                     ANLDALVKEADSGPPDCEAEIAAAERLFTPFVNEKMALALLPAQARDLLGPPERWRAC
                     LLHIRCCIIFGWLLCRRPRNFRLSAYYLYRYWLCVRQVLGTPVTPGRLSDADQRDLAT
                     LVDALAKAWRPYLADELATVDFTAGLPQDLDDGAVDCETGEDETAAIFERLMTLETAQ
                     ALLGAAAFKRESAEPWFWFCRCWCLCSIRFGCCLARARSLVDVLRCLGLYWTCLRECF
                     RPLTCALTGPDGCVAEEVNVDIPALVVPITGTAAGMGFTSYVLEWSRDNITWHASDFV
                     YPPVPPGSTAQGTIPVVSGLLAWLNTTLLDAGTYFVRMTVHGSGNATTTCSIIFSVFK
                     KDVRILGAAGNTSLDAPWTDPAARLIDNVPALCTRPAGTFEASFGGGCLEILGSAWVG
                     GCDNLGTKGYTLDWQRGYVADCTTGSWTNFWAVDFTTPAQQRQINMRTDTSDLTATWG
                     PDCLVPVPFPPYCLLTDPTGKLLPASWNTVLGGACNLSGLFTLRLTVTDTMSGTYCDT
                     QRLWLDNKPISALIQIDAVPKCADLFISQFATPPDCSVPWSLPVSGIAFDELIDNALP
                     ASRPNDNFDYYVVSIEKQGGPSLSIPIPGPGGSCYFGTSRVGDPGVRCDGTPASLLIG
                     TLTTFDLRAIDPTCQGSLPYVVPPGFATPRGQCCVYIFHLTVYDRTSRPCGVNYATSD
                     WPVKICNDLPPN"
     gene            2265..>2497
                     /locus_tag="VIN75_17100"
     CDS             2265..>2497
                     /locus_tag="VIN75_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975939.1"
                     /translation="MDRIWLHLVLAVLATWRLSALLARDDGPWDVIARLRAWLGDGSW
                     GRMLDCFRCVSLWVAAPLAWAVGRDALEQWLAWP"
BASE COUNT          337 a          957 c          810 g          393 t
ORIGIN      
        1 accccgacca tcccccgcgc cgacggcacc gaccccagcc cgccggttcc cgacgatccc
       61 gacttccgca tcgtcctcga gacgctcgtc gacgcctacc ggcccatcct cgaggaagac
      121 ctcaagcgcg ccgccaacct cgatgcactc gtgaaggagg ccgactccgg gccgcccgac
      181 tgcgaggcag agatcgccgc cgccgagcgg ctgttcacgc ccttcgtcaa cgagaagatg
      241 gcgctcgcgc tgctgcccgc gcaggcgcgc gatctgctcg ggccgccgga gcgctggcgc
      301 gcctgcctgc tgcacatccg ctgctgcatc atcttcggct ggctgctttg ccggcgcccg
      361 cgcaacttcc ggctgtcggc ttactacctg taccggtatt ggctctgcgt gcggcaggtc
      421 ctgggcacgc cggtcacgcc cggcaggctg tcggacgccg accagcgcga cctcgcgacg
      481 ctcgtcgacg cgctcgccaa ggcctggcgc ccctacctgg ccgacgagct ggccaccgtc
      541 gatttcaccg ccggcctgcc gcaggacctg gacgacggcg ccgtcgactg cgagaccggc
      601 gaagacgaga ccgccgcgat cttcgagcgc ctgatgacgc tggagaccgc gcaggcgctg
      661 ctgggcgccg ccgcgttcaa gcgcgagagc gccgaaccct ggttctggtt ctgccgctgc
      721 tggtgccttt gctcgatccg tttcggctgc tgcctggcgc gcgcgcgctc gctggtcgac
      781 gtgctgcgct gcctgggctt gtactggaca tgcctgcgcg agtgcttccg cccgctgacc
      841 tgcgccctca ccggccccga cggctgcgtg gccgaggagg tcaacgtgga catccctgcg
      901 ctcgtcgtgc ccatcacggg gacggcggcc ggcatgggct tcaccagcta cgtgctggaa
      961 tggtccaggg acaacatcac ctggcatgcg tcggacttcg tctacccgcc cgtgccgccc
     1021 ggcagcaccg cgcagggcac gatccccgtc gtctcgggcc tgctggcctg gctgaacacg
     1081 acgctgctgg acgccgggac ctacttcgtg cgcatgacgg tgcacggcag cggcaacgcc
     1141 accacgacct gctcgatcat cttctcggtg ttcaagaagg acgtgcgcat cctgggcgcg
     1201 gccggcaaca cgtccctgga cgccccgtgg acggacccgg ccgcccggct gatcgacaac
     1261 gtgcccgcgc tgtgcacgcg cccggccggc accttcgagg cgtcgttcgg cggcggctgc
     1321 ctggagatcc tcggctcggc ctgggtcggc ggctgcgaca acctgggcac caagggctac
     1381 acgctggact ggcagcgcgg ctacgtggcc gactgcacga cgggcagctg gaccaacttc
     1441 tgggccgtcg acttcacgac ccccgcccag cagcgccaga tcaacatgcg caccgacacc
     1501 tcggacctca ccgccacatg gggtccggac tgcctcgtcc cggtgccctt cccgccgtac
     1561 tgcctgctga ccgatccgac cggcaagctc ctgcccgcct cgtggaacac ggtcctgggc
     1621 ggcgcctgca acctcagcgg cctgttcacc ctgcgcctga ccgtcaccga cacgatgtcg
     1681 ggcacgtact gcgacaccca gcggctctgg ctggacaaca agcccatcag cgcgctgatc
     1741 cagatcgacg ccgtgcccaa gtgcgccgac ctgttcatca gccagttcgc cacgccgccc
     1801 gactgctccg tcccgtggag cctgcccgtc tcgggcatcg ccttcgacga gctcatcgac
     1861 aacgcgctgc ccgcatcgcg gcccaacgac aacttcgact actacgtcgt cagcatcgag
     1921 aagcagggcg gcccgtcgct gtcgatcccg atcccggggc cgggtggcag ctgctacttc
     1981 ggcacgtcgc gggtcggcga tccgggcgtg cgctgcgacg gcacgcccgc gtcgctgctg
     2041 atcgggacgc tgacgacctt cgacctgcgc gcgatcgacc cgacgtgcca gggcagcctg
     2101 ccctatgtcg tcccgcccgg cttcgcgacg ccgcgcggcc agtgctgcgt ctacatcttc
     2161 cacctgaccg tctacgaccg cacgtcgcgg ccctgcggcg tcaactacgc cacgtcggac
     2221 tggccggtga agatttgcaa cgacctgccg ccgaactgaa ggccatggac cgcatctggc
     2281 tgcacctggt gctggcggtc ctcgcgacct ggcgcctgtc cgccctcctc gctcgcgacg
     2341 acggcccgtg ggacgtcatc gcgcgcctgc gggcgtggct gggcgacggg tcgtggggcc
     2401 gcatgctgga ctgcttccgc tgcgtcagcc tgtgggtggc cgcgccgctg gcatgggccg
     2461 tcgggcgcga cgcgctggag cagtggctgg cgtggcc
//
LOCUS       DATCRO010000626        10569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_378147, whole genome shotgun sequence.
ACCESSION   DATCRO010000626 DATCRO010000000
VERSION     DATCRO010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378147"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1491)
                     /gene="rho"
                     /locus_tag="VIN75_17105"
     CDS             complement(229..1491)
                     /gene="rho"
                     /locus_tag="VIN75_17105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8975940.1"
                     /translation="MHLSELKAMHIGALITMGEALEIDNVARLRKQELMFAIMKKRAK
                     GGEQVFGDGVLEVLPDGFGFLRAIDESYMASTDDIYLSPSQIRRFNLHTGDMIEGEVR
                     VPKDGERYFALVKVDRVNGVTPEESKHKIMFENLTPLFPKEVFRLERDIRGEENITSR
                     IIDLIAPIGKGQRALIVAQPKTGKTEMMKSIAHALVGNHPEIHLIVLLVDERPEEVTE
                     MQRTVRGEVISSTFDEPAARHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLSGGVDANALQRPKRFFGAARNVEEGGSLTIIGTALIDTGSKMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSIQINRSGTRREEMLLKPEILQKTWILRKLLYPMDE
                     IEAMEFMLDKMKASKTNLDFFDMMRRGG"
     gene            complement(1863..2195)
                     /gene="trxA"
                     /locus_tag="VIN75_17110"
     CDS             complement(1863..2195)
                     /gene="trxA"
                     /locus_tag="VIN75_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY8975941.1"
                     /translation="MSSEAIKHISDASFETDVLKSGTPVLVDYWAEWCGPCKAIAPIL
                     EEVAKDYGPRLQIAKMNVDDNRDVPAKFGIRGIPTLMLFKDGQLAATKVGALSKSQLT
                     AFLEGHLA"
     gene            2495..4276
                     /locus_tag="VIN75_17115"
     CDS             2495..4276
                     /locus_tag="VIN75_17115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY8975942.1"
                     /translation="MVATVPPSTAPAAAPATQNRAVRMLGRYQLMRLLGKSAQTMLWL
                     ALDSRGDVEVMLAMPRARPVDPDALDAWIARVTRSGRLKHPRLARILDVGEHERWPYA
                     VYEREQFVTWGEKLTSKGMPAQELTHWSVQVLQGLAFAHEAGSAHRDLQPYSLLVDDA
                     GNVKLLGLEIVDAHSFAASFIAVSPDPDGHQAAMDERRMQRLAAEIDVLSFGLVMHQV
                     LAGNAPLDEPDVARVVGRLPPFGHEMVRLPWTVPQPIPEPLRAIVNRATDRQERQRYR
                     SARTFERALEGWLQATADQDAGPITLLLGRMRSVGLLPAMPGGADRAARLALLESGHT
                     HELSGVVLQDFALSFELVRMVNFAQSQAGHSGSGAVLAIHRAIAMVGLDGVRQAALSL
                     RPWPGPLNDEAANALTALILRVKRAGNIARELQPAGYDQEVVYLITALQNLGWLVVQY
                     HFPDEAAQIRRLMQPVAAQKEGEPDEQGMTGEGASFAVLGIDIDALGSAVMHHWGLDV
                     GVQHMVRRAPPTGPIHTPTSDVDMLRLVASCANDVLDAVALPARQSAAAIQRVAQRYG
                     RALNITLKDIQDALQPPAAARPDRADA"
     gene            4332..6830
                     /locus_tag="VIN75_17120"
     CDS             4332..6830
                     /locus_tag="VIN75_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY8975943.1"
                     /translation="MSAVPQSARQGGRRVGRFELKGELGRGAQATVWLGYDPRLQREV
                     AVKVINPDADAASVAQWLDEARAVSRLAHPNVVPVFEADQDGAVSFMVFEFVKGPTLT
                     DLLKKRGKLPEREAATLMRDVLDAIATAHAQGIVHRDLKPSNILLDAQGRPRVMDFGI
                     AARVADAHDGRIAGTPGYISPEAIAGAKPHPLMDVFAAGMMLGQMICGRPLLVERDPY
                     RMLERTRDEDIAWPPEMVDAKSGKDATDDALRTLVMRAVSRDPARRPESAAAFRNALH
                     EWLDPAPVGADGGDRATLEFLLRRMRHKSDFPALSDAVVRIQRVTQSENASIAGVTDE
                     IVRDVALTNKLLRMVNTVHFRNAGAGSIASVPRAVSLVGLAGIRSMALSLVLLEHMQD
                     KAHVGRLKEQFLQAMMAGTLCDELTPTAKAHDEPFLAGMMAQLGRMLTEFYFPEEAAL
                     IRRRVEPSWQRGEWSQAIEDRAVGEVLGLSYEQLGVGVTKVWGLPDTLRRAISRPQGE
                     APARPVDNAADRLRWCVQASSEIAQALMQTPGEHAASKISAIGDRYARALNLPKDQFE
                     SAVRGAQSHLQSMAAGLGIDLKADSRARRLMPADLEVPTGNVNPATIVTNVGPASTPD
                     DKTIVTPPGQRPGAAAPGPAPAPATVTTNIAPDSPARMLALENGISSVTSALAGDSFK
                     LNEVLRTILQTMHGALGFRCVVFGLRDPKTGVITGRVGLGGPAPALSAKFRVDVNPNG
                     PVDLLSVACKKAADTLISDASAENVRTRLPTWLQREADARSFVLLPMTMKSAPFALIY
                     ADCASAGGIVLGERELSLLRTLRNQAVMAFKTAG"
     gene            complement(6939..9566)
                     /locus_tag="VIN75_17125"
     CDS             complement(6939..9566)
                     /locus_tag="VIN75_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo 1,3/1,4-beta-D-glucan glucohydrolase"
                     /protein_id="HEY8975944.1"
                     /translation="MPFPTLPRPHRPAAALLAGVLALAPAAHGTDASPAFAPQARQEA
                     QIRDIVAHMTLAQKVGQMTQAEIRAITPDEVRQFYVGSVLDGGGSWPDHDKHASFEDW
                     RKLADALWDASMHTDMAVKVPVIWGTDAVHGHNNVVGMTLFPHNIGLGAANDPALVER
                     IGEAVARQVRLTGVDWTFAPTVAVPRDDRWGRTYEGFSEDPAIVAAYAGRYVTGLQGR
                     FDPAGRPTIVATAKHFIGDGGTDLGIDQGENKAGFADLMRIHGAGYVPAIRAGVQTVM
                     ASFNSWTYTGADDTGRALAFDNVKALGNRYLLTDLLKGRWGFDGFVVTDWDAIGQVTW
                     VDAAGKTHACTTSSCPAAIDAGADMVMVPFEWKAFIANTIASVQAGEIPMARIDDAVT
                     RILRVKMRAGLFRVVDGASVSTAPSQRPGARDAAAAQPRALARQAVRESLVLLKNDRR
                     TLPLARTGRVLVVGRSADSLADQSGGWSVTWQGTGNTNADYPAGESVLAAVRATVGAG
                     NVVHSADAKDVDVKGFKAVIAVIGETPYAEGVGDIRRSSTLEHARRHPEDLAVLERVS
                     GHGVPVVTVLFSGRPLWVNREIDRSDAFVAAWLPGTEGQGITDVLFRHADGGVTDFRG
                     RLSYSWPRDACQATVNKGDANYHPLFAHGYGLTYAKPHAALGKLDESAQPQRCGAAPS
                     RAVEDLVIFDRVEGPMGKLGIGSPANWIVPLGDDPDAVVATKDGAVKAEPAQVNVQQD
                     ARRITFAGHGQFWTYAGSGHDLSGWQGANGAVAFDLVVDHPPEGDVNVRVECGFPCGG
                     SIDVTAAVRALPLHTRATLKIPLQCFVQQGLDLSGVDLPFAIDTDRPFVASLANIRWQ
                     VGGGVDPDALPCTPAKKKP"
     gene            9694..>10569
                     /locus_tag="VIN75_17130"
     CDS             9694..>10569
                     /locus_tag="VIN75_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975945.1"
                     /translation="MNELASTFLETYAAGAEPEGGWTYGKALQQAKLDYTPDSLKRLD
                     ALLSQIRDRARPTRAQLETEAGRNFESLVVFYLIEYAQRVSHAQLQWHDLESARRALP
                     PGVALEDSPVNRLVVDAPANAALFRPLEWLETRLFGEGEAVAPANYVASVLAQLGRDA
                     PPVWFTTMFAVGRLGSWNMMMAASGQGVWPALVNGKAPTSLRPLERGDLRQAVAHCFH
                     LLEDNPDDEAWRVASYPGYAEHAGRKVDAVIVMGASYGPQPVRLAVAFPFRPARDGRP
                     LVILQPSLVETNLPIE"
BASE COUNT         1496 a         3724 c         3764 g         1585 t
ORIGIN      
        1 gagcgggcga tcctgtccaa tcgggcaggt aaagctgcct ccaggcgggc ctttcgggac
       61 atgacgcgga gcgagagctc caccgtgccg gcgctgccgc tcgctcgtcc ccgagaagtt
      121 cgagggagcg cgggaaccgg ctccgccggg cctgtcgcgg tcggccccct cggaggggca
      181 gcgagcgcgc gcgaagcctc ccctccgggg gatagcgagc gtgggggctc aacctcctcg
      241 gcgcatcatg tcgaagaagt ccaggttggt cttgctggcc ttcatcttgt cgagcatgaa
      301 ctccatcgcc tcgatctcgt ccatcgggta gagcagcttg cgcaggatcc aggtcttctg
      361 gaggatctcc ggcttgagca gcatctcctc gcggcgggtg cccgagcggt tgatctggat
      421 cgacgggtag acgcgcttct cggccatgcg gcggtccagg tggatctcgc agttgccggt
      481 gcccttgaat tcctcgtaga tgacttcgtc catcttcgag ccggtgtcga tcagggccgt
      541 gccgatgatg gtcagcgagc cgccttcctc gacgttgcgg gccgcgccga agaagcgctt
      601 ggggcgctgc agcgcgttgg cgtcgacgcc gccggacagc accttgccgg acgacggcag
      661 cacgttgttg tacgcgcggg ccaggcgggt gatcgagtcc agcaggatca ccacgtcctt
      721 cttcatctcg accaggcgct tggcgcgctc gatcaccatc tcggcgacct gcacgtggcg
      781 cgcggccggc tcgtcgaagg tggaggagat gacctcgccg cgcacggtgc gctgcatctc
      841 ggtcacttct tccgggcgct cgtcgacgag cagcacgatc aggtggatct cgggatggtt
      901 gccgaccagc gcgtgcgcga tggacttcat catctcggtc ttgccggtct tgggctgggc
      961 gacgatcagc gcgcgctggc ctttgccgat cggcgcgatc aggtcgatga tgcggctggt
     1021 gatgttctcc tcgccgcgga tgtcgcgctc gagcctgaag acctctttcg ggaagagcgg
     1081 cgtcaggttc tcgaacatga tcttgtgctt gctctcctcg ggcgtgacgc cgttgacgcg
     1141 gtcgaccttg accagcgcga agtagcgttc gccgtccttg gggacgcgca cttcgccttc
     1201 gatcatgtcg ccggtgtgca ggttgaagcg gcggatctgg ctgggggaga ggtagatgtc
     1261 gtcggtggac gccatgtagc tctcgtcgat ggcgcgcagg aagccgaagc cgtccggcag
     1321 cacttcgagc acgccgtcgc cgaacacctg ttcgccgccc ttggcgcgct tcttcatgat
     1381 cgcgaacatc agctcctgct tgcgcaggcg ggccacgttg tcgatctcga gggcttcgcc
     1441 catcgtgatc agcgcaccga tgtgcatggc cttcagttcg gaaagatgca ttgtttcttc
     1501 accctggggt gtgagccgcg gctgaggaca gctcgccgga cggcttgccc gacctgctgc
     1561 aagtgcgcct cgcgctggcc gatccgggct accagtgcac gcgggttgtg cgtgcgctgg
     1621 ccgggtcgag gccgtcgtgg cgacgtgaaa cggtaggggc ttgggacgaa tcgccgaaac
     1681 cgcgcgccgg aatccgccgg actgcggcgg ggctcagggc gcgggggcgc taccttgact
     1741 cggtgcgggc gccgctgctc gtcagaacgg ggcaccagtt actgcactgg tcagggccga
     1801 ttataggggg cggccgagga cgcccgtcaa ctgcgagttg acgaacgtcc gccgcgggtc
     1861 gatcaggcca ggtggccttc gaggaacgcc gtcagctgcg acttggacag cgcgccgacc
     1921 ttggtggccg cgagctggcc gtccttgaac agcatcagcg tgggaatgcc gcggatgccg
     1981 aacttggcgg gcacgtcgcg gttgtcgtcg acgttcatct tggcgatctg caggcgcggg
     2041 ccgtagtcct tggcgacttc ctccaggatc ggggcgatcg ccttgcaggg gccgcaccac
     2101 tccgcccagt aatccaccag caccggggtg cccgatttca ggacgtcggt ctcgaaggac
     2161 gcgtcggaga tgtgtttgat tgcttcgctg ctcatgcgtt ttccttgggg caccggcggc
     2221 aggagttcat cgggaaactc gcattgcccg tcgtcggtga aaagattctg acacaaccgc
     2281 ccgtccccga ccggggtggg gacagcggcg gcgacgcttc ccgctgtaca gccgatctgg
     2341 tggcgatcgg cccgattgga aggggccggc cgacaggcaa ttgaaaactt tagggccgcc
     2401 cgacagtgaa ttacgcatcg ttttcggcgc tcttttgccc gtcgggcctc ttcggccagg
     2461 gttccaatcg accgatcgtt ccgtcattgc gctcatggtc gccaccgtcc ctccctccac
     2521 cgcgcccgcc gccgcacccg ccacgcagaa ccgcgcggtg cgcatgctcg gccgctacca
     2581 gctgatgcgc ctgctcggca agagcgcgca gaccatgctg tggctcgcgc tggacagccg
     2641 cggcgacgtc gaggtcatgc tggcgatgcc gcgcgcccgg ccggtcgacc cggacgccct
     2701 ggacgcctgg atcgcccgcg tcacccgcag cggccgcctg aaacatccca ggctggcccg
     2761 catcctggac gtcggcgagc acgagcgctg gccgtacgcc gtctacgagc gcgagcagtt
     2821 cgtcacctgg ggcgagaagc tcacgtccaa ggggatgccg gcgcaggagc tgacccactg
     2881 gagcgtgcag gtgctgcagg gcctggcctt cgcgcacgag gccggctccg cgcaccgcga
     2941 cctgcaaccg tactcgctgc tggtggacga cgccggcaac gtcaagctgc tgggcctgga
     3001 gatcgtcgac gcccattcgt tcgccgcgtc cttcatcgcc gtctcgccgg atccggacgg
     3061 ccaccaggcc gcgatggacg agcgccgcat gcagcgcctg gccgccgaga tcgacgtgct
     3121 gtccttcggc ctggtcatgc accaggtgct ggccggcaac gcgccgctgg acgagcccga
     3181 cgtcgcgcgc gtcgtcggcc gcctgccgcc gttcggccac gagatggtgc gcctgccgtg
     3241 gacggtgccc caaccgatcc ccgagccgct gcgcgccatc gtcaaccgcg ccaccgaccg
     3301 ccaggagcgc cagcgctacc gcagcgcccg taccttcgag cgcgcgctgg aaggctggct
     3361 gcaggccacc gccgaccagg acgccggccc gatcacgctg ctgctcgggc gcatgcgctc
     3421 ggtcggcctg ctgcccgcga tgcccggcgg cgccgaccgc gccgcgcgcc tggcgctgct
     3481 ggaatcgggc cacacgcacg agctgtccgg cgtcgtcctg caggacttcg cgctgtcgtt
     3541 cgagctggtg cgcatggtca acttcgcgca gtcgcaggcc ggccacagcg gcagcggcgc
     3601 cgtgctggcg atccaccgcg cgatcgcgat ggtgggcctg gacggcgtgc gccaggcggc
     3661 gctgtcgctg cgcccgtggc ccggcccgct caacgacgag gcggccaacg cgctcaccgc
     3721 gctgatcctg cgtgtcaagc gcgccggcaa catcgcgcgc gagctgcagc cggccggcta
     3781 cgaccaggag gtggtgtacc tcatcaccgc gctgcagaac ctcggctggc tcgtcgtgca
     3841 gtaccacttc ccggacgagg ccgcgcagat ccgccgcctg atgcagccgg tggccgcgca
     3901 gaaggaaggc gagcccgacg aacagggcat gaccggcgag ggcgcgtcct tcgccgtgct
     3961 gggcatcgac atcgacgccc tcggatccgc cgtgatgcac cactggggcc tggacgtcgg
     4021 cgtgcagcac atggtgcgcc gcgcgccgcc caccgggccg atccacacgc ccacgtccga
     4081 cgtcgacatg ctgcggctgg tggccagctg cgccaacgac gtgctcgacg ccgtggcgct
     4141 gcccgcgcgc cagtcggccg ccgcgatcca gcgcgtcgcc cagcgctacg gccgcgcgct
     4201 gaacatcacg ctcaaggaca tccaggacgc gctccaaccc ccggcggccg ccaggccgga
     4261 tcgggcggac gcgtgaccca cgccttgcac ccggcgtccg cgcccgcgcg cgtcgcgcgc
     4321 ggaggcgcgc gatgagcgcc gtgccgcagt cggcgcgcca aggcggccgc cgcgtcggcc
     4381 gcttcgagct caagggcgag ctcggccgcg gcgcgcaggc caccgtgtgg ctgggctacg
     4441 acccgcggct gcagcgcgag gtcgccgtca aggtgatcaa tcccgacgcc gacgccgcat
     4501 cggtggcaca gtggctggac gaggcgcgcg cggtcagccg gctcgcgcat ccgaatgtgg
     4561 tgccggtgtt cgaggccgac caggacggcg ccgtgtcgtt catggtgttc gagttcgtca
     4621 agggcccgac gctgaccgac ctgctcaaga agcgcggcaa gctgcccgaa cgcgaggccg
     4681 ccacgctgat gcgcgacgtg ctggacgcga tcgccaccgc gcatgcgcag ggcatcgtgc
     4741 accgggacct caagccgtcc aacatcctgc tggacgcgca gggccgcccg cgcgtgatgg
     4801 acttcggcat cgccgcgcgc gtggccgacg cgcacgacgg ccgcatcgcc ggcacgccgg
     4861 gctacatctc gcccgaggcg atcgccggcg ccaagccgca tccgctgatg gacgtgttcg
     4921 cggccggcat gatgctgggc cagatgatct gcggccgccc gctgctggtc gaacgcgacc
     4981 cataccgcat gctcgagcgc acgcgcgacg aggacatcgc ctggccgccc gagatggtgg
     5041 acgccaagtc gggcaaggac gccaccgacg acgcgctgcg cacgctcgtc atgcgcgccg
     5101 tctcgcgcga cccggcccgc cggcccgaat cggccgccgc gttccgcaac gcgctgcacg
     5161 aatggctcga ccccgcgccc gtgggcgccg acggcggcga ccgcgcgacg ctggagttcc
     5221 tgctgcggcg catgcgccac aagagcgatt tccccgcgct gtcggacgcc gtcgtgcgca
     5281 tccagcgcgt gacgcagtcg gagaacgcga gcatcgccgg cgtcaccgac gagatcgtgc
     5341 gcgacgtggc gctcaccaac aagctgctgc gcatggtcaa caccgtgcac ttccgcaacg
     5401 cgggtgccgg ctcgatcgcg tcggtacccc gtgcggtctc cctggtcgga ctggccggga
     5461 tccgctcgat ggcgttgtcg ctggtgctgc tggagcacat gcaggacaag gcgcacgtcg
     5521 gccggctcaa ggagcagttc ctgcaggcga tgatggccgg cacgctgtgc gacgagctca
     5581 cgcccaccgc caaggcgcac gacgagccgt tcctggccgg catgatggcg cagctcgggc
     5641 gcatgctcac cgagttctac ttccccgagg aggcggcgct gatccggcgc cgcgtcgaac
     5701 cctcgtggca gcgcggcgaa tggtcgcagg cgatcgagga ccgcgccgtc ggcgaggtgc
     5761 tgggcctgtc gtacgagcaa ctgggcgtgg gcgtgaccaa ggtgtggggc ctgcccgaca
     5821 ccctgcggcg cgccatctcg cgcccccagg gcgaggcgcc ggcacgtccc gtcgacaacg
     5881 cggccgaccg cctgcgctgg tgcgtgcagg cctcgtcgga gatcgcgcag gcgctcatgc
     5941 agacgccggg cgagcatgcg gccagcaaga tctcggcgat cggcgaccgc tacgcacgcg
     6001 ccctgaacct gcccaaggac cagttcgaga gcgcggtgcg cggcgcgcag tcgcacctgc
     6061 agtcgatggc cgcgggcctg ggcatcgacc tgaaggccga ttccagggcg cgccgcctga
     6121 tgccggccga cctcgaggtg cccaccggca acgtcaaccc cgccacgatc gtgaccaacg
     6181 tcgggcccgc gagcacgccg gacgacaaga cgatcgtgac gccgcccggc cagcgcccgg
     6241 gcgccgctgc gcccgggccc gccccggcgc cggccaccgt caccacgaac atcgcgcccg
     6301 attccccggc ccgcatgctg gcgctcgaga acggcatctc cagcgtgacg agcgcgctgg
     6361 ccggcgacag cttcaagttg aacgaggtgc tgcgcacgat cctgcagacc atgcacggcg
     6421 cgctcggctt tcgctgcgtc gtcttcggcc tgcgcgatcc caagaccggc gtcatcacgg
     6481 gccgcgtcgg cctgggcggt cccgcgccgg cgctgtcggc caagttccgc gtcgacgtca
     6541 acccgaacgg gccggtcgac ctgctgtcgg tggcctgcaa gaaggcggcc gacacgctga
     6601 tctccgacgc ctccgccgag aacgttcgca cgcgcctgcc gacctggctg caacgcgagg
     6661 ccgacgcgcg cagcttcgtc ctgctgccga tgacgatgaa gagcgcgccc ttcgcgctga
     6721 tctacgccga ctgcgcgagc gccggcggca tcgtgctggg cgagcgcgag ctgagcctgc
     6781 tgcgcacgct gcgcaaccag gcggtgatgg cgttcaagac ggcggggtag ggcgagctgg
     6841 ccgcaggcgt ggcccgccat gtcggcgtgc catggcacgg gcaggaacgc gcgtcgcgat
     6901 gccagcgaac ggcgggttac gcccgcggct aacccgacct acggtttctt cttcgccggc
     6961 gtgcacggca atgcatcggg atcgacgccg ccgcccacct gccagcggat gttggccaag
     7021 ctcgcgacga aggggcggtc ggtgtcgatg gcgaacggca ggtcgacgcc cgacaggtcc
     7081 aggccctgct ggacgaagca ctgcagcggg atcttcagcg tggccctggt gtgcagcggc
     7141 agcgcgcgca cggccgccgt cacgtcgatc gacccgccgc agggaaagcc gcactcgacc
     7201 cgcacgttca cgtcgccctc gggcggatgg tcgacgacga gatcgaaggc caccgcgccg
     7261 ttggcgccct gccagccgct caggtcgtgg ccggatccgg cgtaggtcca gaactggcca
     7321 tggcccgcga acgtgatgcg gcgcgcgtcc tgctgcacgt tgacctgcgc gggctcggcc
     7381 ttgacggcgc cgtccttcgt ggcgacgacg gcgtcggggt cgtcgccgag cggcacgatc
     7441 cagttggccg gcgagccgat gcccagcttg cccatcgggc cttcgacgcg atcgaagatc
     7501 accaggtcct cgaccgccct ggacggcgct gcgccgcagc gttgcggctg ggccgactcg
     7561 tcgagcttgc cgagcgcggc gtgcggcttc gcataggtca ggccgtatcc atgggcgaac
     7621 agcgggtggt agttcgcgtc gcccttgttg accgtggcct ggcaggcgtc gcgcggccag
     7681 gaatacgaca gccggccgcg gaagtcggtg acgccgccgt cggcatggcg gaacaggacg
     7741 tccgtgatgc cttgcccctc ggtgccaggc agccacgcgg cgacgaaggc gtcgctgcgg
     7801 tcgatctcgc gattgaccca caatggccgg ccgctgaaca gcacggtgac gacgggcacg
     7861 ccgtggccgc tcacgcgctc cagcacggcc aggtcctcgg gatggcggcg cgcgtgttcc
     7921 agcgtgctcg agcggcggat gtcgccgacg ccctcggcat acggcgtctc gccgatcacc
     7981 gcgatgaccg ccttgaatcc cttcacgtcg acgtccttcg cgtccgctga atggaccacg
     8041 ttgccggcgc ccaccgtcgc gcgcacggcc gcgaggaccg actcgcccgc cgggtagtcg
     8101 gcattcgtgt tgcccgtgcc ctgccaggtg accgaccagc cgccggactg gtccgccagc
     8161 gagtcggcgc tgcggcccac gaccagcacg cggccggtgc gcgccagcgg cagcgtgcgg
     8221 cggtcgttct tgagcagcac cagcgactcg cgcacggcct ggcgcgcgag cgcgcgcggc
     8281 tgcgcggccg ccgcgtcccg cgcgcccggg cgctgcgagg gcgcggtcga cacgctcgcg
     8341 ccgtccacga cgcggaacag gcccgcgcgc atcttcacgc gcaggatgcg ggtgacggcg
     8401 tcgtcgatgc gcgccatggg gatctcgccg gcctgcaccg aggcgatcgt gttcgcgatg
     8461 aaggccttcc actcgaacgg caccatcacc atgtctgcgc ccgcatcgat ggccgccggg
     8521 cacgaggacg tggtgcacgc atgcgtcttg cccgcggcgt cgacccaggt cacctggccg
     8581 atggcgtccc agtcggtgac gacgaagccg tcgaagcccc agcgcccctt gagcaggtcg
     8641 gtgagcaggt agcggttgcc gagggccttc acgttgtcga acgccagggc gcggccggtg
     8701 tcgtcggcgc ccgtgtaggt ccagctgttg aacgacgcca tcacggtctg cacgccggcg
     8761 cggatcgccg ggacgtagcc ggcaccgtgg atgcgcatga ggtcggcgaa gccggccttg
     8821 ttctcgccct ggtcgatccc caggtcggtg ccgccgtcgc cgatgaagtg cttggcggtg
     8881 gccacgatgg tgggccgtcc ggccgggtcg aagcggccct gcaggccggt gacgtagcgg
     8941 ccggcgtagg cggcgacgat ggccggatcc tccgagaagc cttcgtacgt gcggccccag
     9001 cggtcgtcgc gcggcacggc cacggtgggc gcgaaggtcc agtcgacgcc ggtcaggcgt
     9061 acctgccgcg cgacggcttc gccgatgcgc tcgaccagcg ccgggtcgtt ggccgcgccg
     9121 agcccgatgt tgtgcgggaa cagcgtcatg ccgaccacgt tgttgtggcc gtgcacggcg
     9181 tcggtgcccc agatcaccgg caccttgacc gccatgtcgg tgtgcatgga ggcgtcccac
     9241 agcgcgtcgg cgagcttgcg ccagtcctcg aacgacgcgt gcttgtcgtg gtcgggccag
     9301 gagccgccgc cgtccagcac ggaacccacg tagaactgcc gcacctcgtc gggcgtgatc
     9361 gcgcggatct cggcctgcgt catctggccc accttctgcg ccagcgtcat gtgcgcgacg
     9421 atgtcgcgga tctgtgcctc ctggcgggct tgcggtgcga aggcgggcga tgcgtccgtg
     9481 ccatgggcgg ccggggcgag cgccaggacg ccggcgagca gcgcggcagc cggccggtgc
     9541 gggcgcggca gggtggggaa gggcatcgcg tctcccttgt cgtggttcct gcctggaacc
     9601 gattccaacg caggagggcg actataggac gggacgcgcc gcggcgaaag ggggggcgtg
     9661 gcccggcgcg gatgccacgt taaagtggcg cgcatgaacg aactcgcctc gacattcctg
     9721 gagacctacg ccgccggcgc cgagcccgag ggcggctgga cctatggcaa ggcgctgcag
     9781 caggccaagc tggactacac gcccgacagc ctcaagcgcc tggatgcgct gctgtcgcag
     9841 atccgcgatc gcgccaggcc gacgcgcgcg cagctcgaga ccgaggccgg acgcaatttc
     9901 gagtcgctcg tggtcttcta cctgatcgag tacgcgcagc gcgtctcgca tgcgcagctg
     9961 caatggcatg acctcgaatc ggcgcgccgc gccttgccgc cgggcgtcgc gctcgaggac
    10021 tcgcccgtca accgcctggt cgtcgacgcg ccggcgaacg ccgccctgtt ccggccgctc
    10081 gagtggctgg agacccggtt gttcggcgag ggggaggcgg tggcgccggc gaactacgtc
    10141 gccagcgttc tcgcgcaact cggacgcgac gcgccgcccg tctggttcac cacgatgttc
    10201 gcggtggggc ggctgggctc gtggaacatg atgatggccg cctccgggca gggcgtgtgg
    10261 cccgcgctgg tcaacggcaa ggcgccgacg agcctgcggc ccctggaacg cggcgacctg
    10321 aggcaggccg tcgcgcattg cttccacctg ctggaggaca acccggacga cgaggcctgg
    10381 cgggtcgcca gctacccggg ctacgccgag cacgccggcc gcaaggtcga cgcggtgatc
    10441 gtcatgggcg cgagctacgg gccgcagccg gtgcggctcg cggtcgcgtt cccgttccgt
    10501 ccggcgcgcg atggccggcc gctggtgatc ctgcagccgt cgctggtgga gacgaacctg
    10561 ccgatcgag
//
LOCUS       DATCRO010000627         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_535954, whole genome shotgun sequence.
ACCESSION   DATCRO010000627 DATCRO010000000
VERSION     DATCRO010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535954"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VIN75_17135"
     CDS             <1..378
                     /locus_tag="VIN75_17135"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIP domain-containing protein"
                     /protein_id="HEY8975946.1"
                     /translation="STVFVGDETSLGLALALCGTPLGGLDTHFIFEVSDAAEVRGVLD
                     AMGRGMLQHAWLVERRPDGTHLAQVEAALQRYAGADSFRQYVLSGRSLTIQRLQRVLK
                     AAGAKPSQVLAKAYWAPGKVALD"
     gene            387..1067
                     /locus_tag="VIN75_17140"
     CDS             387..1067
                     /locus_tag="VIN75_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975947.1"
                     /translation="MNRIALVEDHERLATLVRKALAGAGIEVDVFDRIASARHAIAET
                     PYGAVVVDRGLPDGDGLSLVRRMREDGNVTPCLMLTARDAVHDRVEGLEAGADDYLAK
                     PFSMEELAARVRALLRRPAAMQSLTPSHGDIRLAPDQGTMSCGEETVTLPSAELQIML
                     CLVKAEGMVVRRGALEAAAWGLTEAVTPNALDVALHRLRKKLLAIDSTLQVVNVRGHG
                     YALRSDAQ"
     gene            1057..>2378
                     /locus_tag="VIN75_17145"
     CDS             1057..>2378
                     /locus_tag="VIN75_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY8975948.1"
                     /translation="MRNEPRGARRPWRDSDWRHWDAGTTSPDRAADAHAERREQRWRR
                     RAAWHLVHYRRHHQGESLGKRLLATFILASLLSILLAALGLETAFRWHPTIGVTHALG
                     AQADWIESHLAFDAKGVPVALNEPPDTSWVYGTAAHDWKFRVVDGRGVALLSSDAGAP
                     PLAPAGAAFDAGRERFDIVADGLTMHVVTQAQSRGDRPYWIQVAISDRAGKLFSGAMV
                     GPILQNALVIAAIALLLFAGGVQAMLHHLLLPLRRASDAASRISPRSLETRLSEAAMP
                     RELQPLIRAFNEALDRLQQGYRVQQEFLAAAAHELKTPLALIRGQVELSASEDRELLL
                     GDIDRMARQVHQLLHLAEVSESHNFRMSELDLGAVAREAVDFLQRLAQRAGVHIDLRV
                     ADALAPCRADRGALFVLLKNLIENAIQHSPRDAVVTVEVRANGLSVRDA"
BASE COUNT          316 a          868 c          875 g          319 t
ORIGIN      
        1 tccacggtgt tcgtcggcga cgagacctcg ctgggcctcg cgctcgcgct gtgcggcacg
       61 ccgctgggcg ggctcgacac gcacttcatc ttcgaggtgt cggacgcggc cgaggtgcgc
      121 ggcgtgctcg acgcgatggg ccgtggcatg ctgcagcatg cgtggctggt ggagcgccgg
      181 cccgacggca cgcacctggc gcaggtggag gcggcgctgc agcgctacgc gggcgccgac
      241 tcgttccgcc agtacgtgct cagcggccgg tcgctgacga tccagcggct gcagcgcgtg
      301 ctcaaggcgg cgggcgcgaa gccgtcgcag gtgctcgcga aggcctactg ggcgccaggc
      361 aaggtcgcac tcgattgatt ggatccatga accgcatcgc cctcgtcgaa gaccacgaac
      421 gcctggccac gctggtgcgc aaggcgctgg ccggggccgg catcgaggtc gacgtgttcg
      481 accgcatcgc cagcgcgcgc cacgcgatcg ccgagacgcc gtacggcgcc gtggtggtcg
      541 accgcggcct gcccgacggc gacggcctgt cgctggtgcg ccgcatgcgc gaggacggca
      601 acgtcacgcc ctgcctgatg ctgaccgcgc gcgacgcggt gcacgaccgc gtcgagggcc
      661 tggaggccgg cgcggacgac tacctcgcca agccgttctc gatggaggag ctcgcggccc
      721 gcgtacgcgc actgctgcgc cggcccgccg cgatgcagag cctgacgccg tcgcacggcg
      781 acatccgcct ggcgcccgac cagggcacga tgagctgcgg cgaggagacc gtcacgctgc
      841 cgtcggccga gctgcagatc atgctgtgcc tggtcaaggc cgagggcatg gtcgtgcgcc
      901 gcggcgcgct cgaggccgcg gcctggggcc tgaccgaggc cgtcacgccc aacgcgctcg
      961 acgtcgcgct gcaccggctg cgcaagaagc tgctggcgat cgattcgacg ctgcaggtcg
     1021 tcaacgtgcg cggccacggc tacgcgctgc gttccgatgc gcaatgaacc gcgcggcgcg
     1081 cgccgcccct ggcgcgacag cgactggcgc cactgggacg ccggcaccac cagtccggac
     1141 cgcgctgccg acgcacatgc cgagcggcgc gagcagcgct ggcgccgccg cgccgcctgg
     1201 cacctggtgc actaccgccg ccaccaccag ggcgagagcc tgggcaagcg gctgctggcc
     1261 accttcatcc tcgcctcgct cctgagcatc ctgctggccg ccctcggcct ggagacggcg
     1321 ttccgctggc accccacgat cggcgtgacc cacgcgctgg gcgcgcaggc ggactggatc
     1381 gagtcgcacc tggcgttcga cgcgaagggc gtccccgtcg cgctgaacga gccgccggat
     1441 acctcctggg tgtacgggac ggcggcgcac gactggaagt tccgcgtggt cgacgggcgc
     1501 ggcgtggcgc tgctgtcctc cgatgccggc gcccccccgc tggcgcccgc gggcgccgcc
     1561 ttcgacgccg ggcgcgaacg cttcgacatc gtcgccgacg ggctgaccat gcacgtcgtc
     1621 acgcaggcgc agtcgcgcgg cgaccggccg tactggatcc aggtggccat cagcgaccgc
     1681 gcgggcaagc tgttcagcgg cgcgatggtg gggcccatcc tgcagaacgc gctggtgatc
     1741 gcggcgatcg cgctgctgct gttcgccggc ggcgtgcagg cgatgctgca ccacctgctg
     1801 ctgccgctgc ggcgcgcgtc ggacgcggcc tcccgcatct cgccgcgcag cctggagaca
     1861 cgcctgtcgg aagccgcgat gccgcgcgag ctgcagccgc tgatccgcgc gttcaacgag
     1921 gcgctcgacc gcctgcagca gggctaccgc gtgcagcagg agttcctcgc cgcggccgcg
     1981 cacgagctca agacgccgct ggcgttgatc cgcggccagg tggagctgag cgcgtccgag
     2041 gatcgcgagc tgctgctggg cgacatcgac cgcatggcgc gccaggtgca ccagctgctg
     2101 cacctggccg aggtcagcga gtcgcacaac ttcaggatga gcgagctgga cctgggcgcc
     2161 gtggcccgcg aggcggtcga cttcctgcag cgcctcgcgc agcgcgccgg cgtgcacatc
     2221 gacctgcgcg tggcggacgc gctcgcgccg tgccgcgccg accgcggcgc gttgttcgtg
     2281 ctgctgaaga acctgatcga gaacgcgatc cagcattcgc cccgcgacgc ggtggtgacc
     2341 gtcgaggtcc gcgccaacgg gctgtcggtg cgcgatgc
//
LOCUS       DATCRO010000628         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_951415, whole genome shotgun sequence.
ACCESSION   DATCRO010000628 DATCRO010000000
VERSION     DATCRO010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951415"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..123
                     /locus_tag="VIN75_17150"
                     /pseudo
     CDS             16..123
                     /locus_tag="VIN75_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
     gene            complement(294..1109)
                     /locus_tag="VIN75_17155"
                     /pseudo
     CDS             complement(294..1109)
                     /locus_tag="VIN75_17155"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1422..3272
                     /locus_tag="VIN75_17160"
     CDS             1422..3272
                     /locus_tag="VIN75_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8975949.1"
                     /translation="MNTFRQLSIQRKLILSMGFCLVVFLLISGGLGARLIGDAVRDRA
                     VGEELPTAVRAVREEVLRQVTGPLTAARGVAGNSYLQAWEQQGLPDDGAAGWQAYAKQ
                     LKAQEKAATVFWVSKETGKYYTDGGLTRQIGPQDTWFTDFLSGGKPYRLDIDRDNPSA
                     PYMLFINVRTSTADGKLGVAGLGLSIDGLAKSIGDVHIAQTGTAFLVRSNGALMMHAD
                     PSLVDGKHFLKDLPGLDDAVARSLLVQSPFTLAHAGGDLVASSYIPELDAYVLARVPE
                     AELMGPVNRAIRVATLVAAVAGGLVALLVIVVVSRAIARPVRRAADLLAEIADGRGDL
                     TRRMTVESNDEIGQLASAFNRFVGSLSELVRQVRASSESIATGSSQIAVGNLDLSQRT
                     EEQASNLQQTAASMEQLTETVRRNADNAVTASGIVAGAADSAAHGGEVVRDVVRTMEQ
                     ITDSSRRIADIVGVVDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRSA
                     EAAREIKSLIATSVENVESGASRVADAGRAMDTIVDQVAKAKALVGEISEVSLDQSQG
                     IGQVGSAVQQLDQVTQQNAALVEESASAAESLKYQAARLQELMAAFHVAD"
BASE COUNT          544 a         1186 c         1270 g          499 t
ORIGIN      
        1 gcgcgcgtgc tgcgcatgac gcgcgacgag tggcgcaact acatctgctg cgtgctcacc
       61 gtgcgcgagg agctgatccg cgagaaccgg ccgctggtgc aggacctcgt gaacatggtc
      121 gcgggcgcgg gcctctggct cgaccaggcg ccggccaatc gcgagaaggc cgtgcagatc
      181 gccgccggcc gcaagttctt caaccaggat cccaagatcc tcgagttcgt gatgcagaac
      241 tcgaggatct tcgggtcctg gttgaagaac ttgcggccgg cggcgatctg caccgccttc
      301 tcgcggttga ccggtgtctg gtcgagccag agtccggcgc ccgcgaccat gttcacgaga
      361 tcctgcacca ggggccggtt ttcgcggatg agttcctccc gcacggtgag cacgcagcag
      421 atgtagttgc gccactcgtc gcgcgtcatg cgcagcacgc gtgcgtaacc cgcgcgctgc
      481 gcggaggcgc cgaagggctc gcccgtgcag tacgcatcga cggccttggc gtagagcgcg
      541 gcgggcatgt cggcgggcgc catctccacc agcacgatgt ccttgggcga catgccttcc
      601 ttggccagca tcttgcgcag gaacaggaaa tccaccgcga agcggctggg cacggcgatg
      661 cgtttgccgg tgaggtcgcg aaacttctgg tacggcgagt cggtgcgcac catgatgact
      721 gcgccggagc ggtggcccag cgacacgatc ttgaccggga cgcgcttgtc ggcaagatcc
      781 atgaccagcg gcgccagcat gtacgcggcc tggatgcggc cggacatcag cgattccttg
      841 acctcgggcc agccgttgta gcggctgtac tcgtaggcaa gcgcgctgga gcccgacttc
      901 tggttgagcg cgctgcgcga cgtgcaggcg atgggcagcg tgaggttgca ggtgacggcc
      961 aggccgccga cgacgagcgg atcggaggac gcctgcgcgc gcgacggcag cagcaggccg
     1021 ggcagcgcgg cggccatgcc ggcgccggcg gcggtacgca gcgcgctgcg gcggccgggg
     1081 tggtcggggg aggtcggatc aggacgcatc gggcggaggt ggaacgggtt ccagcacact
     1141 gtgacagctc aacctcaagt gtccatcggg actggcggca gcgggccgat gtcgcgacat
     1201 caattcgaaa aaatgtaggt acgtaggtgc aaattcggcg tcgccgcccc atggcgccag
     1261 gccgccggac cgtccaggcc cgacgcggga tcgcgccggg atccgccggc atgccggcat
     1321 cgcgcgcaag cgatgtcgag gcggccgcgg cgagaacttt tccctcaaag ccttgcggcg
     1381 agttgccgat accgacaagg gaccctcatg tggatcacgc aatgaacacc ttccgccagc
     1441 tctccatcca gcgcaagctc atcctcagca tgggcttctg cctggtcgtg ttcctcctga
     1501 tctcgggcgg cctcggcgcg cgcctcatcg gcgacgccgt gcgcgaccgc gccgtcggcg
     1561 aggaactgcc cactgccgtg cgcgcggtgc gcgaggaggt gctgcggcaa gtgaccgggc
     1621 cgctgaccgc cgcgcgcggc gtggcgggca acagctacct gcaggcgtgg gagcagcagg
     1681 ggctgcccga tgacggcgcc gccggctggc aggcctatgc gaagcagctc aaggcgcagg
     1741 agaaggcggc gaccgtgttc tgggtctcga aggagacggg caagtactac accgacggcg
     1801 gactcacgcg ccagatcggt ccgcaggaca cgtggttcac cgacttcctg tccggcggca
     1861 agccgtaccg cctggacatc gatcgcgaca acccgtcggc gccgtacatg ctgttcatca
     1921 acgtgcgcac gtccacggcc gacggcaagc tgggggtcgc gggcctgggc ctgtccatcg
     1981 acggcctggc caagtccatc ggcgacgtcc acatcgcgca gacgggcacc gcgttcctcg
     2041 tgcgctccaa cggcgcgttg atgatgcacg ccgacccgtc gctcgtcgac ggcaagcatt
     2101 tcctgaagga cctgccgggc ctggacgacg ccgtcgccag gagcctgctc gtgcaaagtc
     2161 cgttcacgct cgcccatgcc ggcggcgacc tcgtggcgtc ctcgtacatc cccgagctcg
     2221 acgcctacgt gctggcgcgc gtgcccgagg ccgagctgat gggccccgtc aaccgcgcga
     2281 tccgcgtggc cacgctggtg gccgccgtcg ccggcgggct cgtcgcgctg ctggtgatcg
     2341 tcgtcgtgag ccgcgccatc gcgcgtcccg tgcgccgcgc ggccgacctg ctggccgaga
     2401 tcgccgacgg ccgcggcgac ctgacgcgcc ggatgacggt ggaatcgaac gacgagatcg
     2461 gccagctcgc gtccgcgttc aaccgcttcg tggggtcgct gtcggagctg gtgcgccagg
     2521 tgcgcgcgag cagcgaatcg atcgccaccg gctcgtcgca gatcgcggtg ggcaacctcg
     2581 acctgagcca gcgcaccgag gagcaggcga gcaacctgca gcagaccgcc gcgtcgatgg
     2641 agcagctgac cgagacggtg cgccgcaacg ccgacaacgc ggtgaccgcg tcgggcatcg
     2701 tcgccggcgc cgccgacagc gccgcgcacg gcggcgaggt ggtgcgcgac gtcgtgcgca
     2761 cgatggagca gatcaccgac agctcgcgcc gcatcgccga catcgtgggc gtcgtcgacg
     2821 gcatcgcgtt ccagaccaac atcctcgcgc tgaacgcggc cgtcgaggcg gcgcgcgcgg
     2881 gcgagcaggg gcgcggcttc gcggtggtgg ccggcgaggt gcgctcgctg gcgcagcgct
     2941 cggcagaggc cgcgcgcgag atcaagtcgc tgatcgccac cagcgtcgag aacgtcgaga
     3001 gcggcgcgtc gcgcgtcgcc gacgccggcc gcgcgatgga caccatcgtc gaccaggtgg
     3061 ccaaggccaa ggcgctggtc ggcgagatca gcgaggtgag cctggaccag tcccagggca
     3121 tcggccaggt cggcagcgcc gtccagcaac tcgaccaggt gacgcagcag aacgcggcgc
     3181 tggtggagga gagcgcgtcc gccgccgaga gcctgaagta ccaggcggcg cggttgcagg
     3241 agctgatggc ggcgttccac gtcgcggatt gagccccgcg cgccgcgagc cggagggggg
     3301 ctggcccctc cttcatccgc ggggcgacct tggcttgcgc cacgtcgctt cttcgcggat
     3361 gaaggccggg gcctgacccc gatcgcacgc cggggcgccg catcggcacg cgttcggggt
     3421 caggccttcg cctctgaatc gcgctcggcc ggacggctgt cgtctgcatc ccggcgcgat
     3481 tccgaggaga ggccagacc
//
LOCUS       DATCRO010000629         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_40639, whole genome shotgun sequence.
ACCESSION   DATCRO010000629 DATCRO010000000
VERSION     DATCRO010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40639"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..907)
                     /locus_tag="VIN75_17165"
     CDS             complement(5..907)
                     /locus_tag="VIN75_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8975950.1"
                     /translation="MTGDIRSGEMSEAATAIGAIELPTGTTELATAAAAEALPLALKP
                     TPRRVLVTGATRGIGAAITRALAGAGWHVTLAGRARAALEEVRVTLPIAEGVAHDCVE
                     LDVTDAASVARAFAEVHAKGGALQALVNNAGAVETGPMKSLELASWERMLAVNLTGAF
                     LCTQAALPPMIAARAGRIVNIASTAGQKGYAYCTAYAAAKHGVLGMTRSLALEVAAQG
                     LSVNAVCQGYTDTAIVRDGVARIVAATGRSEADAMATFSQSSPLKRLVEPDEVAATVL
                     WLCTDAPAAFTGQAMSVSGGEVMN"
     gene            complement(981..3335)
                     /locus_tag="VIN75_17170"
     CDS             complement(981..3335)
                     /locus_tag="VIN75_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY8975951.1"
                     /translation="MRIVCIGGGPAGLTFALLMKKLDPGHDITVVERNRPYDTFGWGV
                     VFSDATMAHLRRWDEETAGAIEAAFAHWDDIALEFKGRVIRSGGHGFVGIGRKRLLNI
                     LQARCESLGVTLEFDREVRSDTEFPDADLVVASDGINSVVRQKYADVFKPDIVTRPNR
                     FIWLGTRKLYDAFTFVFERTEHGWFQAHVYKFDAQTSTFIVECPEHVWLAHGLDKLDA
                     DASVAFCEQVFARHLQGAKLMTNARHLRGSAWLNFQRIKCEQWWVGADRREAGSGHAP
                     LVLMGDAVHTAHFAIGSGTKLALEDAIELANQFRQAAGAAGEVSASDLPALLDRYQAL
                     RAVEVLRLQNAAWNAMEWFEVCGARYCDPLEPEQFMYSLLTRSQRISHENLRLRDAGW
                     LEGYERWFAQRGTSEVRVQEPVPPMFTPFTVRSITLKNRVVVSPMAQYSAIDGTVGDY
                     HLVHLGARAMGGAALVMAEMTCTSADGRITKGCPGLYTDAQGQAWARIVDWVHTHTDA
                     HIGMQLGHAGAKGATNLGWEGIDQPLDSGGWPLVSASAQQYLPGVSDWSRAMTRADMD
                     RVLHDFVACTRRAADAGFDWLELHCAHGYLLSSFISPLTNHREDAYGGTLANRLRYPL
                     EVFAAVRAAWPAHLPMSVRISSHDWVEGGITPADAVQVARAFKEAGADLIDCSSGQVS
                     KQEKPVYGRMFQTPFADRIRNEAGIATIAVGAISEADHVNSIIAAGRADLCAVARPHL
                     ANPAWTLTEAARIGYRNVNWPPPYRAAKPQLEAIFQRQHAQGQS"
BASE COUNT          471 a         1225 c         1209 g          511 t
ORIGIN      
        1 gagctcaatt catcacctcc ccaccgctga ccgacatcgc ctgcccggtg aacgccgccg
       61 gcgcatcggt gcacagccac agcacggtcg cggcgacctc gtcgggctcg acgaggcgct
      121 tgagcgggct cgactgcgag aacgtcgcca tcgcgtcggc ctcgctgcgc cccgtggcgg
      181 cgacgatcct ggccacgccg tcgcgcacga tcgccgtgtc ggtgtagccc tggcacaccg
      241 cgttgaccga cagcccctgc gccgcgacct ccagtgccag cgagcgcgtc atgcccagca
      301 cgccgtgctt ggccgccgca tacgccgtgc agtacgcata gcccttctgc cccgccgtgc
      361 tcgcgatgtt gacgatgcgc ccggcgcgcg ccgcgatcat cggcggcagc gcggcctgcg
      421 tgcacaggaa cgcgcccgtg aggttgacgg ccagcatgcg ttcccagctc gcgagttcga
      481 ggctcttcat cgggcccgtc tcgaccgcgc cggcgttgtt gaccagcgcc tgcagcgcgc
      541 cgcccttggc atgcacctcc gcgaacgcgc gcgcgacgct cgcggcgtcg gtcacgtcca
      601 gctcgacgca gtcgtgcgcg acgccttcgg cgatcggcaa ggtcacgcgc acctcctcga
      661 gcgcggcgcg cgcgcggccc gcgagcgtca cgtgccagcc cgcgcccgcg agcgcgcgcg
      721 tgatggccgc gccgatgccg cgcgtggcgc ccgtcaccag cacgcggcgc ggcgtcggct
      781 tgagcgcgag cggcagggcc tcggcggctg ccgccgtcgc cagctcggtg gtgccggtgg
      841 gcagctcgat cgcgccgatc gccgtggcgg cctcgctcat ctcgccgctg cggatgtcgc
      901 cggtcatggg gtcaccctcc gcccggcggc agcggtgtga cgggcgcagg agggagcgcc
      961 tgccgggggg cgcgcggtgc tcatgactgc ccctgcgcgt gctggcgctg gaagatcgcc
     1021 tccagctgcg gcttggccgc gcggtacggc ggcggccagt tcacgttcct gtagccgatg
     1081 cgcgcagcct cggtcagcgt ccacgccggg ttcgcgagat gcggccgcgc cacggcgcac
     1141 aggtcggccc ggccggcggc gatgatgctg ttgacgtggt cggcctcgct gatggccccc
     1201 accgcgatcg tcgcgatgcc cgcctcgttg cggatgcggt ccgcgaacgg cgtctggaac
     1261 atgcggccgt agaccggctt ttcctgcttg ctgacctggc cggacgagca gtcgatcagg
     1321 tcggcgccgg cctccttgaa ggcgcgcgcg acctgcaccg cgtcggccgg cgtgatgccg
     1381 ccctcgaccc agtcgtgcga cgagatgcgc accgacatcg gcaggtgcgc cggccacgcg
     1441 gcgcgcaccg ccgcgaagac ttcgagcgga taacgcagcc ggttcgccag cgtgccgccg
     1501 tacgcgtcct cgcgatggtt cgtcagcggc gagatgaacg acgacagcag gtagccgtgc
     1561 gcgcagtgca gctccagcca gtcgaagccg gcgtcggccg cccggcgcgt gcaggcgacg
     1621 aaatcatgca gcacgcgatc catgtcggcg cgggtcatgg cgcgcgacca gtcgctcacg
     1681 cccggcaggt actgctgcgc cgaggccgac accagcggcc agccgcccga gtccagcggc
     1741 tggtcgatgc cttcccagcc gaggttcgtc gcgcccttgg caccggcgtg gcccagctgc
     1801 atgccgatgt gcgcgtcggt gtgcgtgtgc acccagtcga cgatgcgcgc ccaggcctgc
     1861 ccttgcgcat cggtgtacag gccggggcag cccttggtga tgcggccgtc ggcgctggtg
     1921 caggtcatct cggccatcac cagcgcggcg ccgcccatcg cgcgggcgcc caggtgcacg
     1981 aggtggtagt cgccgacggt gccgtcgatc gccgagtact gcgccatcgg cgagacgacc
     2041 acgcggttct tcagcgtgat cgagcgcacc gtgaacggcg tgaacatcgg cggcacgggc
     2101 tcctgcacgc gcacctcgct cgtgccgcgc tgcgcgaacc agcgctcgta gccttcgagc
     2161 cagccggcgt cgcgcagccg caggttctcg tggctgatgc gctggctgcg cgtgagcagc
     2221 gagtacatga actgctcggg ctccagcggg tcgcaatagc gcgcgccgca gacctcgaac
     2281 cactccatcg cgttccacgc cgcgttctgc agccgcagga cctcgacggc gcgcagggcc
     2341 tggtagcgat cgagcagggc aggcaggtcc gacgccgaca cctcgcccgc agccccggcg
     2401 gcctgcctga attgattggc gagctcgatc gcatcttcca gcgcgagctt ggtgcccgag
     2461 ccgatcgcga agtgcgcggt gtgcacggcg tcgcccatca acaccagcgg cgcgtggccg
     2521 gagccggcct cgcggcggtc ggcgccgacc caccactgct cgcacttgat gcgctggaag
     2581 ttcagccacg ccgagccgcg caggtggcgc gcgttggtca tgagcttggc accctgcagg
     2641 tggcgcgcga agacctgctc gcagaaggcg acggacgcgt cggcgtcgag cttgtccagg
     2701 ccgtgcgcga gccacacgtg ctcggggcat tcgacgatga aggtcgacgt ctgcgcatcg
     2761 aacttgtaga cgtgcgcctg gaaccagccg tgctcggtgc gctcgaagac gaacgtgaac
     2821 gcgtcgtaga gcttcctggt gcccagccag atgaagcggt tgggccgcgt gacgatgtcg
     2881 ggcttgaaga cgtccgcgta cttctggcgc accaccgagt tgatgccgtc gctggcgacc
     2941 acgaggtcgg cgtcggggaa ttcggtgtcg gagcgcacct cgcggtcgaa ctccagcgtc
     3001 acgcccaggg actcgcagcg cgcctgcagg atgttgagca ggcgcttgcg gccgatgccc
     3061 acgaagccgt ggccgcccga gcggatcacg cggcccttga actccagcgc gatgtcgtcc
     3121 cagtgcgcga acgcggcctc gatggcgccg gccgtctcct cgtcccagcg gcgcaggtgc
     3181 gccatcgtgg cgtcggagaa caccacgccc catccgaagg tgtcgtaggg gcggttgcgc
     3241 tcgaccacgg tgatgtcgtg ccccgggtcg agcttcttca tcagcagggc aaaggtgagg
     3301 ccggcggggc cgccgccgat gcagacgatg cgcattgaag tcctcgtcta tcctggagag
     3361 cgctggcgcc ggcgactgcc cggcgccaat tgcttgaggt atgaatagtt taatac
//
LOCUS       DATCRO010000630         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_805453, whole genome shotgun sequence.
ACCESSION   DATCRO010000630 DATCRO010000000
VERSION     DATCRO010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805453"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1112)
                     /locus_tag="VIN75_17175"
     CDS             complement(33..1112)
                     /locus_tag="VIN75_17175"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY8975952.1"
                     /translation="MSQHPPARVQQRERGHLADGRVVHEVTLSHGGLTLSFIALGGIV
                     TTLWAPDRDDVAVNVVRGFDDLADYERRNPFFGVIVGRYANRIALGELAIDGETFALS
                     RNNGTNCLHGGTHGWGTRLWEIESLREHDDALGGPAATLRYASADGEEGFPGRVQARV
                     TYSLGADRSWRIDYEATTDRATVVNLSHHDYFNLSGHGTALDHVLTLPAGRYNPVDGK
                     LIPMGIAEVDDTPFDFRAPTTIRARMDDGHPQLAAGGGYDHNWILDDARIGTLRLAAR
                     LEDPASGRAMELHTTEPALQFYSGNFLDGSLAGHGGEVLQRGAAVCLEPQHSPDSPHH
                     AQWPSTVLRPGQAYRSCSVYRFFAR"
     gene            complement(1109..2521)
                     /locus_tag="VIN75_17180"
     CDS             complement(1109..2521)
                     /locus_tag="VIN75_17180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8975953.1"
                     /translation="MSTVSQLPGTPDPDAPPPGLLRLAWPIFAEQSLHMLTNVVSTLM
                     ASRIGDNAVAALGSAGQVLVMFLIGFNVLAVGASIATTHHLGLGDRSGARRMARGAVA
                     TNLWLGAIFSLLLAGTAPTLLRWAQLTPPLMAYALPYLHWMGGTLFMESMIFALCAVL
                     RAHGHTREVMYVILAQNAVNAGLSALLIFGLGGLPALGVTGIAIAASVSRLLAVIALW
                     MLVSRRIGLKLRAHDLVDIHLADLRRLLSLGFPAVLENISWFASFMIITALTARMGER
                     ALATQAYTMQIAQIEMLACLSIGIGNEIVVGRLIGAGRFEDARRQCLVNMRLGLLVTA
                     GVAIVFALAAPWALRMFTDDPQIVSLGRTLILLGLLLEPGRSFNLIIINALRASGDSR
                     FPLIAGLLSQWGIMLLGAWLLGTHLGLGLIGVWAALILDEWLRGLYMLQRWRHGRWLP
                     HARRVQAEAAQHPLQSSTFT"
     gene            2862..3794
                     /locus_tag="VIN75_17185"
     CDS             2862..3794
                     /locus_tag="VIN75_17185"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8975954.1"
                     /translation="MTAPRQPARYQGVFPVVPTIFDERGGLDLAGQKRCVDFMIDAGS
                     NGLCILANFSEQFVLADDERETLTRTILEHVAGRVPVIVTTSHFSTDICLARGQRAQA
                     QGAAMVMVMPPYHGATLRVSDKQIHHFFGRLSEGLDIPIMIQDAPMAGTPLSAAFLAR
                     LAQDHERVRYFKIETAGAASKLRELIELGGDAIEGPWDGEEAITLLADLDAGATGAMT
                     GGGYPDGIRTIVDAFAAGRREEAVEAYGRWLPLINFENRQGGFLSAKALMKEGGIIAC
                     DAPRSPYPAMSPHVRAGLLDAARRLDPLVLRWGR"
     gene            3842..4576
                     /locus_tag="VIN75_17190"
     CDS             3842..4576
                     /locus_tag="VIN75_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY8975955.1"
                     /translation="MSERAEHSPYRTKPAGKSMHGRIVRELGQRILAGELAPGDRLPP
                     ESELIDKYEVSRPVLREAVRVLVAKGLVLSRQRAGAIVRPRNEWHLLDPDVLYWMIQT
                     RPAPEFVHTLMGLRRVFEPGAAAMAAQAATDEQLAAIAQAYDGMAGATHGDAMLEPDL
                     AFHRRIAEATGNDLMAYIGNMMSLALRESIKLSNRVPDTHVLSLPRHKAILVALQNRD
                     AAAAHQATLLQLDATQLDLSAALARD"
     gene            complement(4573..4857)
                     /locus_tag="VIN75_17195"
     CDS             complement(4573..4857)
                     /locus_tag="VIN75_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975956.1"
                     /translation="MLKIRPGAAQRAALPSRPGSGGLATAGTLILASVSAVSLVLLSG
                     CHAAPASRSSSAFAATSADKAAEARLAASDAMPPVQPFDEHLGAELAAGD"
     gene            5008..5763
                     /locus_tag="VIN75_17200"
     CDS             5008..5763
                     /locus_tag="VIN75_17200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975957.1"
                     /translation="MNPTNKVAVVTGAGSGIGKSCALALHRAGYRVVLAGRRAQALEA
                     VAAQAGGGDALLAVPTDVTQPAQVDVLYAATLARFGRVDVLFNNAGISANGIPFEDLT
                     HERWCAVVDTNLTGMFLCAQGAFRAMKAQTPRGGRIINNGSISAHAPRPLSSPYTSTK
                     HAITGLTRASNLDGRAYDIAVGQVDIGNAETPMTERMVGGVLQPDGRMMPEPRMDVKA
                     VGDAVAYMAGLPLSANVLFITVMATKMPFVGRG"
BASE COUNT          959 a         2153 c         2186 g          865 t
ORIGIN      
        1 gtcgcggcag tgaaaaccct cgatcgccgc cttcagcgcg cgaagaagcg atagaccgag
       61 cagctccggt aggcctggcc ggggcgcagc acggtcgagg gccactgcgc gtggtgcggc
      121 gaatcggggc tgtgctgggg ctccaggcac accgcggcgc cgcgctgcag cacctcgccg
      181 ccgtggccgg ccaggctgcc atcgaggaag ttgcccgagt agaactgcag cgccggctcg
      241 gtggtgtgca gctccatcgc gcggcccgaa gcggggtctt ccaggcgtgc cgcgaggcgc
      301 agcgtgccga tgcgcgcgtc gtccaggatc cagttgtggt cgtagccgcc gccggcggcc
      361 agctgggggt ggccgtcgtc catccgcgcg cggatcgtcg tcggcgcgcg gaagtcgaac
      421 ggcgtgtcgt cgacctcggc gatgcccatc gggatgagct tgccgtcgac cgggttgtag
      481 cggccggccg gcagcgtcag cacgtggtcc agcgccgtgc cgtggccgga aaggttgaag
      541 tagtcgtggt ggctcaggtt gacgacggtg gcgcggtcgg tggtggcctc gtagtcgatg
      601 cgccagctgc ggtcggcgcc cagcgagtag gtcacgcgcg cctgcacgcg gcccgggaag
      661 ccttcctcgc cgtcggcgct ggcgtagcgc agcgtcgcgg ccgggccgcc gagcgcgtcg
      721 tcgtgctcgc gcagcgactc gatctcccac agccgcgtgc cccagccatg cgtgccgccg
      781 tgcaggcagt tcgttccgtt gttgcgcgag agcgcgaagg tctcgccgtc gatcgcgagc
      841 tcgcccagcg cgatgcggtt cgcatagcgg cccacgatca cgccgaagaa cgggttgcgg
      901 cgctcgtagt cggcgaggtc gtcgaagccg cgcacgacgt tgacggcgac gtcgtcgcga
      961 tcgggcgccc acagggtcgt gacgatgccg cccagcgcga tgaaggacag cgtcaggccg
     1021 ccgtgcgaca gcgtgacctc gtggacgacg cggccgtcgg ccaggtggcc gcgttcgcgc
     1081 tgctgcactc gggcgggagg atgttggctc atgtgaaggt gctgctctgg agtggatgtt
     1141 gcgccgcctc ggcctggacg cgtcgcgcgt ggggaagcca gcggccgtgc cgccagcgct
     1201 gcagcatgta caggccgcgc agccattcgt ccaggatcag cgcggcccac acgccgatca
     1261 ggccgaggcc caggtgcgtg cccagcagcc aggcgcccag cagcatgatc ccccactgcg
     1321 acagcaggcc ggcgatgagc gggaagcggc tgtccccgct ggcgcgcagc gcgttgatga
     1381 tgatcaggtt gaagctgcgc cccggctcca gcagcagtcc cagcaggatg agcgtgcggc
     1441 ccagcgagac gatctgcggg tcgtcggtga acatgcgaag cgcccacggc gcggccagcg
     1501 cgaagacgat cgccacgccg gccgtcacga gcagccccag gcgcatgttg accaggcact
     1561 gcctgcgcgc gtcctcgaag cggccggcgc cgatgaggcg gcccacgacg atctcgttgc
     1621 cgatgccgat cgacaggcac gcgagcatct cgatctgcgc gatctgcatc gtgtaggcct
     1681 gcgtggccag cgcgcgctcg cccatgcgcg cggtcagcgc cgtgatgatc atgaacgacg
     1741 cgaaccagct gatgttctcg agcaccgcgg gaaagccgag ggacagcagg cggcgcaggt
     1801 ccgccaggtg gatgtcgacc aggtcgtgcg cccgcagctt caggccgatg cggcgcgaga
     1861 cgagcatcca cagcgcgatg accgccagca gcctgctgac cgacgcggcg atcgcgatgc
     1921 cggtcacgcc cagcgccggc aggccgccca gcccgaagat cagcagcgcg ctcaagcccg
     1981 cgttgaccgc gttctgcgcg aggatcacgt acatcacctc gcgcgtgtgg ccgtgcgcgc
     2041 gcagcacggc gcacagcgcg aagatcatcg actccatgaa caacgtgccg cccatccagt
     2101 gcaggtaggg cagcgcgtag gccatcagcg ggggcgtcag ttgtgcccag cgcagcagcg
     2161 tgggcgcggt gcccgccagc agcaggctga agatcgcgcc cagccacagg ttggtggcca
     2221 ccgcgccgcg tgccatgcgc cgcgcgccgg agcggtcgcc caggcccagg tggtgcgtcg
     2281 tggcgatcga cgcgccgacc gccagcacgt tgaagccgat cagaaacatc accagcacct
     2341 gccccgcgct gccgagcgcg gccaccgcgt tgtcgccgat gcggctggcc atcagcgtgg
     2401 acaccacgtt ggtcagcatg tgcagcgact gctcggcgaa gatgggccac gccaggcgca
     2461 gcagcccggg cggcggcgcg tcggggtcgg gcgtgccggg caactgggag acggtcgaca
     2521 tggggcggca gcttagcttc gaatggttac gcgcgattac agggcgcctt ggcgggggtg
     2581 gatcctgcga ctgcgcttcg ctccgcgcgg gatgacggcg ttgttgctgg cgcggggcgt
     2641 gatcggcgcg gccgtcgctg gtgtcgctgg tcgtcggcag tcggcagtcg accgtcgtcg
     2701 gcaccagccc cgtgcggtca tcctgcgcgc agtcacagga tccacgcacc gcgtcacgcc
     2761 gcccataaaa gtcagactat aaatcgacag gaaatccctt atccgcttaa actcgcgcca
     2821 ggttgccaga tatagtcata ctttaatcag gaagcagaga catgaccgct ccccgccagc
     2881 ccgcgcgcta ccagggcgtc ttccccgtgg tgccgacgat cttcgacgag cgcgggggcc
     2941 tggacctcgc cggccagaag cgctgcgtcg acttcatgat cgacgcgggc tccaacggcc
     3001 tgtgcatcct cgcgaacttc tccgagcagt tcgtgctggc cgacgacgaa cgcgagacgc
     3061 tcacgcgcac gatcctcgag cacgtcgccg gccgggtgcc ggtgatcgtg acgacgtcgc
     3121 acttctccac cgacatctgc ctggcgcgcg gccagcgcgc gcaggcgcag ggcgcggcga
     3181 tggtcatggt gatgccgcct taccacggcg ccacgctgcg cgtgagcgac aagcagatcc
     3241 accacttctt cgggcggctg tccgagggcc tggacatccc catcatgatc caggacgcgc
     3301 cgatggccgg cacgccgctg tcggccgcct tcctggcgcg gctcgcgcag gaccacgagc
     3361 gcgtgcgcta cttcaagatc gagacggccg gggcggcgag caagctgcgc gagctgatcg
     3421 agctcggcgg cgacgcgatc gaaggcccgt gggacggcga ggaggccatc acgctgctgg
     3481 ccgacctcga cgccggcgcc accggtgcga tgaccggcgg cggctatccg gacggcatcc
     3541 gcaccatcgt cgacgccttc gccgccggcc ggcgcgagga ggccgtcgag gcctacggcc
     3601 gctggctgcc gctgatcaac ttcgagaacc gccagggcgg cttcctgtcg gccaaggcgc
     3661 tgatgaagga gggcggcatc atcgcgtgcg acgcgccgcg cagcccgtat ccggcgatga
     3721 gcccgcacgt gcgcgccggg ctgctcgatg cggcgcgtcg actcgatcct ctggtcctgc
     3781 gctggggccg ctgaaggtct ccgtgatagc gtcgcggcca tctcccacct ctgcccgaga
     3841 catgtccgaa cgcgccgagc actcgccgta ccgcaccaag cccgcgggca agagcatgca
     3901 cgggcgcatc gtgcgcgaac tgggacagcg catcctcgcg ggcgaacttg cgccgggcga
     3961 ccggctgccg ccggagtccg agctgatcga caagtacgag gtcagccggc ccgtgctgcg
     4021 cgaggccgtg cgcgtgctcg tggccaaggg gctcgtgctc tcgcgccagc gcgccggcgc
     4081 gatcgtgcgc ccgcgcaacg agtggcacct gctcgacccc gacgtgctgt actggatgat
     4141 ccagacccgc cccgcgcccg agttcgtcca taccctcatg ggtttgcgcc gcgtgttcga
     4201 gcccggcgcc gccgcgatgg ccgcgcaggc ggccaccgac gagcagctgg cggccatcgc
     4261 ccaggcctac gacggcatgg ccggcgcgac gcacggcgac gcgatgctgg agcccgacct
     4321 cgcgttccac cgccgcatcg ccgaggccac cggcaacgac ctgatggcct acatcggcaa
     4381 catgatgtcg ctggcgctgc gcgagtcgat caagctgagc aaccgcgtgc ccgacacgca
     4441 cgtgctgtcg ctgccgcggc acaaggccat cctcgtcgcg ctgcagaacc gcgacgcggc
     4501 ggccgcgcac caggccacgc tgctgcagct ggacgcgacg cagctggacc tgtcggccgc
     4561 gctggcacgc gactagtcgc ccgcggccag ttcggcgccc aggtgctcgt cgaacggctg
     4621 caccggcggc atggcgtcgc tcgcggccag gcgcgcctcg gcggccttgt cggccgacgt
     4681 cgccgcgaag gcgctgctgg agcggctggc gggtgccgcg tggcagcccg agagcagcac
     4741 gagcgagacg gccgagaccg aggcgagaat cagcgtgccg gcggtggcca ggccgccgct
     4801 gccgggacgg gaaggaagcg cggcgcgttg cgcggcgccg ggacggatct tcagcatcat
     4861 gcaaccccct gatgcggaca tctggcggcc cgctgcgaac cgtcatcttg aactttcgcg
     4921 cgcatcagca catccctcag gcaggggaca cgcgccgtgt ccggatctgc cgtcagaagt
     4981 ttcaactcga caggaaagca ctcgaccatg aacccgacga acaaggtcgc cgtcgtcacc
     5041 ggcgccggca gcggcatcgg caagtcctgc gcgctggcgc tgcatcgggc ggggtatcgc
     5101 gtcgtcctcg ccggacggcg tgcgcaggcg ctggaggcgg tcgccgcgca ggccggcggc
     5161 ggcgacgcgc tgctggccgt gcccaccgac gtcacccagc ccgcgcaggt cgacgtcctg
     5221 tacgcggcga cgctcgcgcg cttcggccgc gtcgacgtgc tgttcaacaa cgccggcatc
     5281 agcgccaacg gcatcccgtt cgaggacctg acccacgagc gctggtgcgc ggtggtcgac
     5341 accaacctca ccggcatgtt cctgtgcgcg cagggggcgt tccgcgcgat gaaggcgcag
     5401 acgccgcgcg gcggccgcat catcaacaac ggctcgatct cggcgcacgc gccgcggccg
     5461 ttgtcctcgc cctacacctc gaccaagcac gcgatcaccg ggctcaccag ggcctccaat
     5521 ctcgacggcc gcgcctatga catcgcggtc ggccaggtcg acatcggcaa tgccgagacg
     5581 ccgatgaccg agcgcatggt cggcggcgtg ctgcagcccg acggccgcat gatgccggag
     5641 ccgcgcatgg acgtgaaggc ggtcggcgac gccgtggcct acatggccgg cctgccgctc
     5701 agcgccaacg tcctgttcat cacggtgatg gccaccaaga tgccgttcgt gggacggggg
     5761 tgatcgatcc atcgcaacaa ccgttaagac tcattggggc gcttgcagac cggagcggcg
     5821 ccaatgaatg cgccctctcc ccttgcggga gagggcatgt aggaaagcgc ggcgagctcg
     5881 gttgggtgag gggtatctct ccacgagtgt cgttcgcgga gaattacccc tcacccaaca
     5941 gcgtgcgtgg atggatcgga gatgccctct cccgcaaggg gagagggcgc agtcattagc
     6001 atcgcagctc gtctgtggtc tgcaacgcgc tacgttgacg agcctattcg ttgccgaggc
     6061 gctgcgaacc gagaccctac tccagccgct ccaccttgcg taagcccgga aacagcttca
     6121 tccagagcag cgcgacggcg atggtgccgg cgccgccgag cac
//
LOCUS       DATCRO010000631         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_862212, whole genome shotgun sequence.
ACCESSION   DATCRO010000631 DATCRO010000000
VERSION     DATCRO010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862212"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VIN75_17205"
     CDS             complement(<1..345)
                     /locus_tag="VIN75_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8975958.1"
                     /translation="MSALETQALGMVPMVIETSGRGERAYDIYSRLLRERIVFLVGPV
                     NDHSANLVVAQLLFLESENPDKDISLYINSPGGSVSAGMSIFDTMNFIKPNVSTLCIG
                     LAASMGAFLLSAG"
     gene            complement(514..1821)
                     /gene="tig"
                     /locus_tag="VIN75_17210"
     CDS             complement(514..1821)
                     /gene="tig"
                     /locus_tag="VIN75_17210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8975959.1"
                     /translation="MAVNVETLDKLERRISLSLPADEINKEVESRLKKLSRTVKADGF
                     RPGKVPMSVVAQRYGYSVQYEVMNDKLGAAFNAAANEAKLRVAGAPRIAEKEKSAEGS
                     MDFDATFEVYPEVVIGDLSAVEVERVTTTVDDAAIDKTVEILRKQRRTFAQRPAAEGA
                     QAGDRVTIDFEGKIDGEPFEGGKAEAFQFLVGEGQMLEQFDQAVRGMKVGESKTFPLQ
                     FPADYHGAEVAGREADFMVTMKKIEAQHLPEVDDAFAKSLGIADGTVDGLRADVRKNL
                     EREVKFRVLARNKAAAMDALSAAATLDLPKALVQGETERLVANAREDLKQRGVKDADK
                     APIPAEIFVPQAERRVRLGLVVAELVRANNLQAKPEQLQAHIEEMSQSYEKPVEVQRW
                     YLSDRSRMAEVEAIVVENNVTEFVLGKAKVTDKELPFDSLMTA"
     gene            complement(1847..1933)
                     /locus_tag="VIN75_17215"
     tRNA            complement(1847..1933)
                     /locus_tag="VIN75_17215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1897..1899),aa:Leu,seq:tag)
     gene            complement(2031..>5049)
                     /locus_tag="VIN75_17220"
     CDS             complement(2031..>5049)
                     /locus_tag="VIN75_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8975960.1"
                     /translation="FTFRAMVVQAYWPGASAKDVAEQVTDKIERTLQEVPYSDKIRSY
                     TKPGESLTILQLADNSPPHEVANTWYQARKKIGDMRGTLPQGVIGPFFNDEFGDVYGS
                     IFALSADGFSDEELRVRAEDIRSQLLRVHDVAKVQLFGVQSQKIWVELSQKRAAEMGV
                     DVSGVISQLNAQNAVQGSGVVDSGTQNVQVRVAGAFQSVDDIRALPIRVVNPASGQAT
                     SIKLGDIADVRRGYSDPPDVKVRANGKQVIAIGVSMAKGGDIIALGKGLQKQVAEVRK
                     QLPVGMELSQVQDQPEAVSRSVGEFVRVLVEAIVIVLGVSFVSLGLHFKPIRIDIWPG
                     LVVAITIPLVLAITFVTMYYWGVGLHKISLGSLIIALGLLVDDAIIAVEMMVRKLEEG
                     YSKERAATFAYEVTSMPMLTGTLITAAGFLPIGLAKSMTGEYTFAIFAVTSAALLISW
                     FVSVYFVPYLGARLLHTRMKAHEPDGSLSAQPHETFDSPFYERFRRVVAWCIGHRWIT
                     IGATIGIFVLGLVGMGLVQQQFFPDSSRPEIMVDLWLPEGATVKQSEDVAKRFEARML
                     KETGVKSVTTWVGSGVPRFYLPLDQVFPQSNVSEAIVEPTDLKVRESLRKKLPALLAA
                     EFPEIRSRVKLLPNGPPVPYPVQFRVVGADADQVRHWADEAKAVLLANTHMRGVNDNW
                     NENVLAMRLAVDQAKARALGVTTQAIAQATQTQFSGSTIGQYREGDQLIDIVLRQPLA
                     ERSAVTNLSSAYVATSNGGVVPLAQVARLSLGWEPGVLWREGRDYAVTVQGDVVEGMQ
                     GPTVTAELWGKGNAVGPLRALMKKMPPGYEIQVAGAVEESSKGQGAIAAGVPVMLFII
                     FTLLMLQLHSFSRALLVFATGPLGVAGAAGALLALNRPFGFVALLGVIALMGMIMRNS
                     VILIDQIEQDRAHGVPAWDAIIESAVRRFRPIVLTAAAAVLAMIPLSESEFWGPMAVA
                     IMGGLLIATVLTLLALPAMYAAAFRVRPVETVA"
BASE COUNT          774 a         1749 c         1688 g          838 t
ORIGIN      
        1 gcccgccgac agcaggaacg cgcccatgct ggccgccaag ccgatgcaca gcgtcgagac
       61 gttgggcttg atgaagttca tcgtgtcgaa gatggacatg cccgcgctga cgctgccgcc
      121 cggcgagttg atgtacagcg agatgtcctt gtccgggttc tcgctctcca ggaacagcag
      181 ctgcgcgacc accaggttgg ccgaatggtc gttcaccggg cccaccagga agacgatgcg
      241 ctcgcgcagc aggcgcgagt agatgtcgta ggcgcgttcg ccgcggccgg aggtctcgat
      301 gaccatgggc accatgccca gggcttgcgt ttcaagtgcg ctcatggagg agtacttttc
      361 gtcagatgaa aggggtcttt cggagaccat tatgtcgcgt cgggcgcgac cccaccgtcg
      421 gagatggcgg tgaaaccccg cgttgcaagt cgcaacccga aaagacgaac gcccggcagc
      481 gccgggcgtc gtcgacgggg cagacgcgag gcgtcaggcc gtcatcagcg agtcgaacgg
      541 cagttccttg tccgtgacct tggccttgcc cagcacgaac tcggtgacgt tgttctcgac
      601 gacgatcgcc tcgacctcgg ccatgcgcga gcggtccgac aggtaccagc gctgcacttc
      661 caccggcttc tcgtagctct gagacatctc ctcgatgtgc gcctggagct gctcgggctt
      721 ggcctgcagg ttgttggcgc gcacgagttc ggcgacgacc aggcccaggc gcactcggcg
      781 ctcggcctgc ggcacgaaga tctcggccgg gatcggggcc ttgtcggcat ccttgacgcc
      841 gcgctgcttc aggtcctcgc gggcgttggc gaccaggcgc tccgtctcgc cctgcaccag
      901 ggccttgggc aggtccagcg tggcggcggc cgagagcgcg tccatcgcgg cggccttgtt
      961 gcgggccagg acgcggaact tgacctcgcg ctcgaggttc ttgcgcacgt cggcgcgcag
     1021 gccgtcgacc gtgccgtcgg cgatgcccag cgacttggcg aacgcgtcgt cgacctcggg
     1081 caggtgctgc gcctcgatct tcttcatcgt gaccatgaag tcggcttcgc ggccggccac
     1141 ttcggcgccg tggtagtcgg cggggaactg cagcgggaac gtcttggact cgccgacctt
     1201 catgccgcgc accgcctggt cgaactgctc gagcatctgg ccttcgccga ccaggaactg
     1261 gaacgcctcg gccttgccgc cttcgaacgg ctcgccgtcg atcttgcctt cgaagtcgat
     1321 cgtgacgcgg tcgccggcct gggcaccttc ggcggccggg cgctgggcga aggtgcgacg
     1381 ctgcttgcgc aggatctcga ccgtcttgtc gatggcggcg tcgtcgaccg tggtggtgac
     1441 gcgctcgacc tcgacggcgg acaggtcgcc gatgacgact tccgggtaga cctcgaacgt
     1501 ggcgtcgaag tccatcgaac cttcggccga cttctccttc tcggcgatgc gcggggcacc
     1561 ggcgacacgc agcttggcct cgttggccgc ggcgttgaac gcggcgccca gcttgtcgtt
     1621 catgacttcg tactgcaccg agtagccgta gcgctgggcc acgacggaca tcggcacctt
     1681 gcccgggcgg aacccgtcgg ccttgaccgt gcgggacagc ttcttgaggc gggactcgac
     1741 ttccttgttg atctcgtcgg ccgggaggct cagggagatg cggcgctcga gcttgtcgag
     1801 ggtttcaacg ttgactgcca tgatgggact cttggttcgc aaattctggt gcgcggggcg
     1861 ggactcgaac ccgcacgccg gtgaaggcgt caggacctaa acctggtgcg tctaccaatt
     1921 tcgccacccg cgcttcgtga tccaggagct ggggacagga ttggacgcaa accccccgct
     1981 ttccggtgaa ccagagattg tagccggatc cggccgggcg gccggattct tcacgcgact
     2041 gtttccaccg gacgaaccct gaaggcggcg gcgtacatcg ccggcagcgc cagcaaggtc
     2101 agcaccgtgg cgatcagcag gccgcccatg atggccaccg ccatcgggcc ccagaactcg
     2161 ctctcggaca gcgggatcat cgccagcacg gcggccgcgg cggtcagcac gatcgggcgg
     2221 aaccggcgca cggccgactc gatgatcgcg tcccacgccg gcacgccgtg ggcgcggtcc
     2281 tgctcgatct ggtcgatcag gatgaccgag ttgcgcatga tcatgcccat gagcgcgatc
     2341 acgcccagca gcgccacgaa gccgaacggc cggttgagcg ccagcaacgc gccggcggcg
     2401 ccggccacgc ccagcggccc ggtggcgaac acgagcagcg cgcgcgagaa gctgtgcagc
     2461 tgcagcatca gcagcgtgaa gatgatgaac agcatcaccg gcacgcccgc cgcgatcgcg
     2521 ccttgcccct tggacgactc ctcgaccgcg cccgcgacct ggatctcgta gcccggcggc
     2581 atcttcttca tcagcgccct cagcggcccg acggcgttgc ccttgcccca cagctcggcg
     2641 gtgacggtcg gcccctgcat gccctcgacg acgtcgccct gcacggtgac cgcgtagtcg
     2701 cgcccctcgc gccacagcac gcccggctcc catccgagcg agagcctggc gacctgcgcg
     2761 agcggcacca cgccgccgtt gctggtggcc acgtaggcgc tggacaggtt cgtgacggcg
     2821 ctgcgctcgg ccagcggctg gcgcagcacg atgtcgatca gctggtcgcc ctcgcggtac
     2881 tggccgatcg tcgagcccga gaactgcgtc tgcgtggcct gcgcgatggc ctgcgtcgtc
     2941 acgcccagcg cgcgtgcctt ggcctggtcg acggccaggc gcatcgccag cacgttctcg
     3001 ttccagttgt cgttgacgcc gcgcatgtgc gtgttggcga gcagcacggc cttggcctcg
     3061 tcggcccagt ggcgcacctg gtcggcgtcg gcgcccacca cgcggaactg caccgggtag
     3121 ggcaccggcg gcccgttggg cagcagcttg acgcggctgc ggatctccgg gaactcggcg
     3181 gccagcagcg ccggcagctt cttgcgcagg ctctcgcgca ccttgaggtc cgtgggctcg
     3241 acgatggcct cgctgacgtt gctctgcggg aacacctggt ccagcggcag gtagaagcgc
     3301 ggcacgccgc tgcccaccca cgtcgtcacc gacttcacgc cggtttcctt gagcatgcgc
     3361 gcctcgaagc gcttggcgac gtcctcgctc tgcttgaccg tcgcgccttc cggcagccac
     3421 aggtcgacca tgatctcggg ccggctcgag tccgggaaga actgctgctg cacgaggccc
     3481 atgcccacca ggcccagcac gaagatgccg atcgtcgcgc cgatggtgat ccagcgatgg
     3541 ccgatgcacc acgcgaccac gcggcggaag cgctcgtaga aaggcgagtc gaaggtctcg
     3601 tgcggctgcg ccgacaggct gccgtccggc tcgtgcgcct tcatgcgcgt gtgcagcagg
     3661 cgcgcgccca ggtacggcac gaagtacacc gagacgaacc acgagatcag cagcgccgcc
     3721 gacgtcaccg cgaagatcgc gaacgtgtac tcgccggtca tcgacttggc cagcccgatc
     3781 ggcaggaagc ccgccgcggt gatcagcgtg ccggtgagca tcggcatcga cgtcacctcg
     3841 tacgcgaagg tggcggcgcg ctccttcgag tagccctcct cgagcttgcg caccatcatc
     3901 tcgaccgcga tgatcgcgtc gtccacgagc aggcccagcg cgatgatcag cgatcccagc
     3961 gatatcttgt gcagcccgac gccccagtag tacatcgtga cgaaggtgat cgcgagcacc
     4021 agcgggatcg tgatcgccac gaccaggccg ggccagatgt cgatgcggat cggcttgaag
     4081 tgcaggccca ggctcacgaa gctgacgccc agcacgatga cgatcgcctc gaccagcacg
     4141 cgcacgaact cgcccaccga gcggctgacc gcctcgggct ggtcctgcac ctgcgacagc
     4201 tccatgccga ccggcagctg cttgcggacc tcggccacct gcttctgcag gcccttgccc
     4261 agcgcgatga tgtcgccgcc cttggccatg gacacgccga tcgcgatgac ctgcttgccg
     4321 ttggcgcgca ccttgacgtc cggcggatcg gagtagccgc gcctgacgtc ggcgatgtcg
     4381 cccagcttga tgctcgtggc ctggccgctg gccgggttga cgacgcggat cggcagcgcg
     4441 cggatgtcgt cgaccgactg gaaggcgccg gcgacgcgca cctgcacgtt ctgcgtgccc
     4501 gaatcgacca cgcccgagcc ctgcaccgcg ttctgcgcgt tgagctggct gatgacgccg
     4561 ctgacgtcca cgcccatttc ggccgcgcgc ttctgcgaca gctcgaccca gatcttctgc
     4621 gattgcacgc cgaacagctg caccttggcg acgtcgtgca cgcgcagcag ctgcgaccgg
     4681 atgtcctcgg cgcgcacgcg cagctcctcg tcgctgaagc cgtcggccga cagcgcgaag
     4741 atcgagccgt agacgtcgcc gaactcgtcg ttgaagaacg gcccgatgac gccctgcggc
     4801 agcgtgccgc gcatgtcgcc gatcttcttg cgcgcctggt accaggtgtt ggcgacctcg
     4861 tgcggcggcg agttgtcggc cagctgcagg atggtcagcg actcgcccgg cttggtgtag
     4921 ctgcggatct tgtcggagta cggcacctcc tgcagcgtgc gctcgatctt gtcggtcacc
     4981 tgctcggcga cgtccttggc gctcgcgccc ggccagtagg cctgcacgac catcgcgcgg
     5041 aacgtgaac
//
LOCUS       DATCRO010000632         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1042216, whole genome shotgun sequence.
ACCESSION   DATCRO010000632 DATCRO010000000
VERSION     DATCRO010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042216"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VIN75_17225"
     CDS             complement(<1..389)
                     /locus_tag="VIN75_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8975961.1"
                     /translation="MPSAEPDLREAAAAAAHDAATAANDPVARAPRPRAPARPRARGP
                     APAAEGAPAGGGALDDVDRALLRILQADGRITNLKLAQAVGLSSASTQERVKRLLRDG
                     TILSFNARLNPALLQAGLLVFAEVRLE"
     gene            complement(470..979)
                     /locus_tag="VIN75_17230"
     CDS             complement(470..979)
                     /locus_tag="VIN75_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8975962.1"
                     /translation="MNKPQSEPSTVSNLSNDKGIDKIDARILRVLQRDGRISNLKLAE
                     EVHLSPTAVLERVKRLTRENFILGYEARLNPNKLGASMMVFIEVLLDRTAPDIMDTFK
                     AAVQTRPEILECHLVAGGFDYLIKTRVADMAAYREMIASVIWSLPGVRETRTYTVMEE
                     VKTTNALPI"
     gene            1076..>2454
                     /locus_tag="VIN75_17235"
     CDS             1076..>2454
                     /locus_tag="VIN75_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8975963.1"
                     /translation="MSASTSTFVAKPQQARLPYPYRPEVDVVEAALATLTGQLDWAQV
                     IRAGRPWVEAVREKPAPFWAMESLLREYPISSAEGLALMRLAEALLRVPDVETAIALT
                     ADQLGRAEFDADSVVDGPHKMLAGISASAISLSKKFLPDADGEGGLLKRLGAKTVVAA
                     TVRAIQLLGRQFVLGRTIAEAMDEAQDQRKDVESARRGAGTAASGLRFSYDMLGEGAR
                     TEHDAERYLASYANAIKAISRGQVAASPENADGISIKLSALFSRYEDAQRERVFAELL
                     PRVWSLVELAARANLNLTIDAEETDRLELSLDVLDALAERIGAAFPQWQGFGLAVQAY
                     QTRALQVVDAVAAIARKHKLRFMVRLVKGAYWDGEIKRAQEGGLAGYPVFTHKEHTDI
                     SYLACARALIGHADVIYPQFATHNAGTIAAILQMARAKGAAFEMQRLHGMGEGIYREV
                     MKDPAIPCRV"
BASE COUNT          347 a          851 c          872 g          384 t
ORIGIN      
        1 tcctccagcc gcacctcggc gaacacgagc aggccggcct gcagcagcgc cgggttcagg
       61 cgggcgttga agctcaggat cgtgccgtcg cgcagcaggc gcttcacgcg ttcctgcgtg
      121 ctggctgagg acaggccgac cgcctgcgcg agcttgaggt tggtgatgcg gccgtcggcc
      181 tgcaggatgc gcagcagcgc gcggtcgacg tcgtcgagcg cgccgccgcc cgccggcgcg
      241 ccctcggcgg ccggtgccgg gccgcgcgcc cgcggacggg ccggggcgcg cgggcgaggc
      301 gcgcgggcga cggggtcgtt ggccgcggtg gcggcgtcgt gggccgcggc agcggcggcc
      361 tcgcgcaggt cgggttcggc ggacggcatg cccgttacgg tgccgcatcc cctcccgagc
      421 cgttcactga attgccgcca tcgcgccgag caatgtgcgg ccttggcgtt cagatcggca
      481 gcgcgttggt ggtcttgacc tcttccatca ccgtgtacgt gcgcgtctcg cgcacgcccg
      541 gcaggctcca gatgaccgag gcgatcatct cgcggtaagc ggccatgtcg gccacgcggg
      601 tcttgatcag gtagtcgaag ccgccggcca ccaggtggca ctcgaggatc tccgggcgcg
      661 tctgcacggc cgccttgaag gtgtccatga tgtcgggcgc ggtgcgatcc agcagcacct
      721 cgatgaacac catcatgctg gcgccgagct tgttcgggtt cagccgggct tcgtagccga
      781 ggatgaagtt ctcgcgcgtc aggcgcttga cgcgttcgag caccgccgtg ggcgacaggt
      841 gcacctcctc ggcgagcttc aggttgctga tgcgaccgtc ccgctgcaac acgcgcagga
      901 tgcgggcgtc gatcttgtcg attcccttgt cgttcgacag attcgacacc gtggacggtt
      961 cggactgggg cttgttcatg ctggacgaat ctccgggctc tctggcgaaa tccgaattta
     1021 tctcgtggat tctcgctcta agctaccaga ttatccagtc gggatttaca ccgccatgtc
     1081 cgcatccacg tccacctttg tcgcgaagcc gcaacaggct cgcctgccct atccgtaccg
     1141 tccggaggtg gacgtcgtcg aggccgcgct ggccacgctg accgggcagc tggactgggc
     1201 ccaggtgatc cgcgccggcc gcccgtgggt ggaggcggtg cgcgagaagc cggcgccgtt
     1261 ctgggcgatg gagtcgctgc tgcgcgagta cccgatctcg agcgccgagg gcctggccct
     1321 gatgcgcctg gccgaggcgc tgctgcgcgt gcccgacgtc gagaccgcca tcgcgctgac
     1381 ggccgaccag ctcggacgtg ccgagttcga cgccgactcc gtcgtcgacg gcccgcacaa
     1441 gatgctcgcc ggcatctccg ccagcgccat ctcgctgtcc aagaagttcc tgcccgacgc
     1501 cgacggcgag ggcgggctgc tcaagcgcct gggcgccaag acggtggtcg ccgccacggt
     1561 gcgcgccatc cagctgctgg gccgccagtt cgtgctgggc cgcaccatcg ccgaggcgat
     1621 ggacgaggcc caggaccagc gcaaggacgt cgagagcgcg cgccgcgggg ctggcacggc
     1681 cgcgtcgggc ctgcgcttct cgtacgacat gctgggcgag ggcgcccgca ccgagcacga
     1741 cgccgagcgc tacctggcct cgtacgcgaa cgcgatcaag gcgatctcgc gcggccaggt
     1801 cgccgcgtcg cccgagaacg ccgacggcat ctcgatcaag ctgtcggcgc tgttctcgcg
     1861 ctacgaggac gcccagcgcg agcgcgtgtt cgccgagctg ctgccgcgcg tgtggtcgct
     1921 ggtcgaactg gctgcgcgcg ccaacctgaa cctgacgatc gacgccgagg agaccgaccg
     1981 cctggagctg tcgctggacg tgctggacgc gctggccgag cgcatcggcg ccgccttccc
     2041 gcaatggcag ggcttcggcc tggccgtgca ggcctaccag acgcgcgcgc tgcaggtggt
     2101 cgacgccgtc gccgccatcg cgcgcaagca caagctgcgc ttcatggtgc gcctcgtgaa
     2161 gggcgcgtac tgggacggcg agatcaagcg cgcgcaggaa ggcggcctgg cgggctatcc
     2221 ggtgttcacg cacaaggaac acaccgatat ctcctacctg gcctgcgccc gcgcgctgat
     2281 cggccacgcc gacgtcatct atccgcagtt cgccacgcac aacgccggca ccatcgccgc
     2341 gatcctgcag atggcgcgcg ccaagggcgc cgcgttcgag atgcagcggc tgcacggcat
     2401 gggcgagggc atctaccgcg aggtgatgaa ggatccggcg atcccgtgcc gcgt
//
LOCUS       DATCRO010000633        16959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_547359, whole genome shotgun sequence.
ACCESSION   DATCRO010000633 DATCRO010000000
VERSION     DATCRO010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16959
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547359"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VIN75_17240"
     CDS             <1..1140
                     /locus_tag="VIN75_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY8975964.1"
                     /translation="VYGSYAAGVYLTPIAGGMISDRWLGKRRAVIVGGLLMALGHFMM
                     ASEALFFPAMVVIACGNGLFLPNLPSQVKPLYAAGDPRLAGAYNLYYMGINLGGFLAP
                     LVCGTLGELYGWHWGFGAAGVGMCLGLAIYVAGARHLAPVDAADEAGAAPAAPAASRH
                     QILFLAGTALAVVIFRVAYAQTGNTLALWADTAVDRRAFGWTIPATWVQLLNPLYVFL
                     MTPLLVAFWNRRGDAQAQSAPLWKMAIGAMTVAASFALLAAVARASEAGGAPVHWSWL
                     VAFFALLTLGELYILPVGLGLFAQLALPGFGATTIAAWFLAAFAGNLLSGVVGTWWTH
                     LSPDAFFLAMGALAALAGVLLTLLAAAERRWFTDLMTAQRAGNPP"
     gene            1137..2135
                     /locus_tag="VIN75_17245"
     CDS             1137..2135
                     /locus_tag="VIN75_17245"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY8975965.1"
                     /translation="MTTPLARRRVDSMLVWDNHACMPLTPHDTSFLPQLKRHRDAGVD
                     VVMVNVGFGDDGVEQHLRMLAAMRDWLLAHASEYRLIDTVDDIEIARANGQLAVGFDI
                     EGANAIADQLSLLGLYRDLGVRWMLLAYNRNNRAGGGCQDDDAGLSAFGRRMLHEMAR
                     VGIVACCSHTGYRTAREAIDESPTPVIFSHSNAHALVDHPRNIPDDLIKACAARGGVV
                     GVNGIGIFLGNNDASVANVARHVLHMLDLVGPSHVGLGLDYIFDTRDLAAEIAAHPDK
                     FPPHLGYSADIAMTAPEQLPDLADELARRGVDDPSLALIFGGNWLRVAREVWQPPF"
     gene            complement(2227..2646)
                     /locus_tag="VIN75_17250"
     CDS             complement(2227..2646)
                     /locus_tag="VIN75_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975966.1"
                     /translation="MTTYVLSLTGDEEKAANATHWARFRRAKMISVSRKGDQRTFDAG
                     RDSIVIAAHGMGDHRATSCNPSTHDPAALAALLVRTLDIQDGTGVVLADFDNEDFAAD
                     VVARIAALGRHVTCTGQVRDFSFGARVRPLLARGVRS"
     gene            complement(2673..3263)
                     /locus_tag="VIN75_17255"
     CDS             complement(2673..3263)
                     /locus_tag="VIN75_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8975967.1"
                     /translation="MFHTPTDTRTANATAFGRYPAAKPLQFPLAMDLCRDQVPAPADC
                     DGLRLAAAARKSTSTHAFLPAFANADWRVFASFCVARRVPAGFRVMIPGRSDRTLRFV
                     VEGALWQESAAGNRDKVLAAGTIVGEDALFSDGASDLDVRALEDSLILELPLARQKEL
                     AASCPEIGFELLRAGGAVIAAGGRPAVAREEEIAAN"
     gene            3534..4484
                     /locus_tag="VIN75_17260"
     CDS             3534..4484
                     /locus_tag="VIN75_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8975968.1"
                     /translation="MGTLFDPRTGDRVVLRAEHVFGRNALRADTAVDDPGISSMHAVI
                     RWRNGRWMVSDHSTNGTFVNGQVLVPGEPFALEAGVELRLGRGPNVAWRVQDVAAPVD
                     VLVPADLLRPVIVLAAHNLLPSSDAPELCIYLAGPGLWMLEQNGETRPLKDGDAVQMG
                     AGAHRFVAAAPMDDTQVAEVAREPESPLLVMRLSLDEEHASLEVKDGVARTDLGERSH
                     HYCLATLARRRMADQERGLEPGAQGWLASAELSRMLGMETTHVNMQIFRAREQLMTAL
                     PGVAALSRLIERRRGELRIGELPFEIYRGDSLECRYRPLR"
     gene            complement(4466..5803)
                     /locus_tag="VIN75_17265"
     CDS             complement(4466..5803)
                     /locus_tag="VIN75_17265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8975969.1"
                     /translation="MLPERIGRYDITAEIGRGSTSIVYRGIDPRDGRAVAIKTLQRSA
                     DSDGDADEILRRFRDEALAVRRLDHPGLVAIDEIGEDGLNTWLAMPHVEGRTLADMLR
                     GTPPLAEERALAILDELLDALGAAHREGLTHRDVKPANVLLTPAGRVKLVDFGVARLR
                     DLGLTRVSSIIGTPAFMAPEQFTGRALDHRADLFACGALLHQLLAGRPPFCGAPAAVM
                     QQILHEEPPPTSRASGGRADPAYDSVVARALAKRAEDRFASADEMRDALHAAASHAAD
                     APLATVVGTVVGTLVQDAGSLLGHLSPEQRAAFEHARAHRLDPMAGVVLRDAPVLAQR
                     VDAFALYVPDAGSCHAIVDIPHREGADETSAAVPAASAATLPPEACATAQQLLARRVG
                     PVASVMVRRAADTAGGSLDVFVANLLAGTSASQQAALRAELEAALAPPQRSGR"
     gene            complement(6186..6929)
                     /locus_tag="VIN75_17270"
     CDS             complement(6186..6929)
                     /locus_tag="VIN75_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8975970.1"
                     /translation="MIQFSKVNKWYGSYHALVDIDETIAKGEVVVVCGPSGSGKSTLI
                     RTINRLEAIESGTISFDGEDIHRPGIDLNRLRSRIGFVFQQFNLFPHLTVLQNCTLAP
                     IEVGGARPAQAHEKAMALLDRVGLASKANAWPSELSGGQQQRVAIARALAMEPPLMLF
                     DEPTSALDPEMVGEVLLVMQGLVRDGMTLVVVTHEMGFARDVAHRVLFMDQGRILERA
                     EPHAFFNHPQHPRAQQFLADIRTPFARPA"
     gene            complement(6926..7663)
                     /locus_tag="VIN75_17275"
     CDS             complement(6926..7663)
                     /locus_tag="VIN75_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8975971.1"
                     /translation="MIDLIQTYWVYFLIGQYPQGPLGGVAATVLLAALALLLAMPIGL
                     LMGLARTSSIAALRRPVTVVAYLIRGIPLLLMVFWIYFYLPTVTGHKTDQFTTMLIAL
                     VVFDGVYLGEIVRAGIRGVPKGQYEAARALGLGHGGAMRRVILPQALRSMLPSLVNQF
                     VSTIKATSLGYIIGLTEASFIATQVNSQVFVHPTAVYLLLSLMYFTLCFGLSRVALAI
                     ERRQARRDLPRPVHPPGSPTATAAANP"
     gene            complement(7674..8369)
                     /locus_tag="VIN75_17280"
     CDS             complement(7674..8369)
                     /locus_tag="VIN75_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8975972.1"
                     /translation="MPGFDLQLLLHGQYHEWLLAGLLVSLKLAGVTIACALPLAIAIA
                     LLRLSPWPPLQWLGATYVEGIRNVPLIAHLLFWYFGAPELLPDAWKERLYAGSYEFIA
                     AAIGLTLYAAAFMAENIRSGIRAIPAVQFEAARALGLGFIPAMRRVVLPQALRVTVPP
                     LLSQVIILWNDTSVATVIGVGEMMYQAARVESAAFHSVEAFAFVTVSYLAGSLLLTAA
                     ADWIHRRWPVRTL"
     gene            complement(8370..9197)
                     /locus_tag="VIN75_17285"
     CDS             complement(8370..9197)
                     /locus_tag="VIN75_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8975973.1"
                     /translation="MRTNLRSLLAAVLAATTLVSAAHADQLADIKKKGEIVVGVLGTD
                     EPNSFVDPKTREIVGYEVDLANAVARKIGVKATFKQIAVAARIPELQQGRVDLLAASL
                     THNKERESQIDFSLTTFVTGQKVMVKASSGITDVPQLAGKKVLTVKGGTQEENVRKAV
                     PSVDVVTFETTQQAFQALQQGKGAGYVNDEATLVNDMAKLGPARKDYRILPTNLSTEP
                     LALGIRKNEPALKKVVDDTLAELEKSGEADKIFMKWYGPTTKLQFDKRSFKIDSDKI"
     gene            complement(9300..10301)
                     /locus_tag="VIN75_17290"
     CDS             complement(9300..10301)
                     /locus_tag="VIN75_17290"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY8975974.1"
                     /translation="MTASSALRPVAVYGAAGHTARFVLAELRRRGLAAIAVGRNAAKL
                     PTDVPARDAAIDDPAALDAAFAGCAVVINCAGPFLDTAAPVIEAALRGGAHYLDVTAE
                     QASAMDTFERFEAAARAAGVAVIPAAGFYGGLADLLATSLLGADGAADELTVAVALDR
                     WWPTSGTRVTGERNTFPRLVVADGRLAPLPLPAARSTWTFGAPHGKQEVVELTFSEVV
                     TLTRHLRLGAMSAHLTQSSLDEIRDTTTPPPAATDALGRSPQRFEMVVRTRQGDRVRQ
                     ATARGQDIYAVSAPLVVQAAQRLMAPAFARSGAIALGQAVDAADFLRALSPEHLELA"
     gene            10375..10974
                     /locus_tag="VIN75_17295"
     CDS             10375..10974
                     /locus_tag="VIN75_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8975975.1"
                     /translation="MPSKKSSGGDAAGESSDVRQRILDTASRLFYERGVRAVGVDLVV
                     DDSGVAKTSLYRHFRTKDDLIVAFLQREDAEFWAQWDEVHARHAGEPMAELEAHVRWI
                     GKRLARANYRGCPQINVAAEFAEADHPSRAVSREHMQAMRKRLAELARGLGAARPGEL
                     AAQLGVLVNGAFVSAAMLSPEEATRVLLGAARALVAASR"
     gene            11000..11389
                     /locus_tag="VIN75_17300"
     CDS             11000..11389
                     /locus_tag="VIN75_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8975976.1"
                     /translation="MTDHHASCSCGQLRLVAKGDPVRVSMCHCYACQRRTGSTYGAQA
                     RFLTENVRITGTSTEYVRLGESGAAVHFHFCPDCGATVYYWFDDTFRAVPVGAFADNT
                     FPPPRISVWEDRMHPWVSVPEGAERMA"
     gene            11451..11750
                     /locus_tag="VIN75_17305"
     CDS             11451..11750
                     /locus_tag="VIN75_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY8975977.1"
                     /translation="MSDAAPGPWVLYLIECTGERLYAGITNDLAARFAAHRAGRGARF
                     TKAFAPLRVVAASQLPSRSEALKAEHALKRQPRAAKLAFLAACGTPLAPSALPQD"
     gene            11898..14045
                     /locus_tag="VIN75_17310"
     CDS             11898..14045
                     /locus_tag="VIN75_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8975978.1"
                     /translation="MTQAFHSMRWHVPAAAVAAAGSALCAAQQPDAEPQTVTITATRI
                     ATPAFDVPASISLVTLSPDTDSRPGINLSETLAGVPGLLARDRQNYAQDVQISVRGFG
                     ARSTFGIRGVRLYVDGIPATMPDGQGQISNVDLGSAAAVEVLRGPFSALYGNSSGGVV
                     QVFTEDGAGAPRLTVGAGGGSDGQRRYDAKLSGAQGPLDYVASVSRFETDGYREHSAA
                     RRDIQNLKARLQLGDAGKLTIIGNGVQLPSAQDPLGLTRAELQADPRGVDPAATQFDT
                     RKSLSQTQGGLIYELRVNDANALRAMVYDGHRDTTQFQSIPVATQANPLNPGGVIRLG
                     RDYDGADLRWTITGGPAERRWSLIGGAAWDKLREHRQGFQDFTGDPAAPVSGVQGALR
                     RDEINDVEDLDPYVQGQWHPSEDWTLDAGVRRSRVRFVSHDRYVVGANPDDSGQATYE
                     ATLPVLGVQWAASPELHLYATAGRGFETPTLNELAYRPSGLTGLNFDLKPARSESLEI
                     GAKAHSASLGVLDVAVFGTRTRDEIVTLSNVGGRSTYQNVASTRRLGLEVAWTKLLPD
                     DVALQVAYTLLAAQYTDGFLACTTTPCTAPATPVASGNRIPGVARNALSAELGWTPPR
                     GWRASVGARAVGRVFVNDTNSDSASSFVTANVSAGYVLPVGAWNFSAFARVDNVTDRR
                     YVGSVIVNEGNGRYFEPAPGRTFFAGLTTSYRF"
     gene            complement(14078..14881)
                     /locus_tag="VIN75_17315"
     CDS             complement(14078..14881)
                     /locus_tag="VIN75_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8975979.1"
                     /translation="MDLGLKGKAALVCASSKGLGRGCAGALAAEGVDLVLVARGRDAL
                     EATAQALREAAGVKVVAIAADITTDAGRALALDAARELRHGAPAAFDIVVTNAGGPPM
                     GDFRDFDRAAWLEAVEANMLTPIELIKATVDGMAARGWGRIVNVTSSSVKSPIASLSL
                     SNGARSGLTGFVAGVAREVAGRGVTINNLLPGAFATDRLLSSFAPRAQRLGIGTDEVA
                     RQREAAIPAGRFGTPEEFGAACAFLCSAQAGYITAQNVLIDGGAYPGTL"
     gene            14936..15844
                     /locus_tag="VIN75_17320"
     CDS             14936..15844
                     /locus_tag="VIN75_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8975980.1"
                     /translation="MTDAPKRLSIDAGVLRVACLEYGEPAGRPVVLLHGFPYDVHAYD
                     AVAPRLAARGMRVIVPYLRGYGPTRFLSDATPRSGQQAALGADLLALLDGLSLERALV
                     GGYDWGGRAACIVAALWPGRVRGLVSANGYNLQDIARSVEPATPDKEYRLWYQYYLHG
                     ERGRAGLAAHRREFARLLWQLWSPCWKFGDADFERTAPAFDNPDFVDVVVHSYRHRFG
                     LVDGDPAFEGIERRLAAQPAIAVPAITLDGDADGVTPDGTPGPGRFIGRHEHRRLEGG
                     VGHDLPQEAPQAFAQAVLDVDGWAAG"
     gene            complement(15854..16165)
                     /locus_tag="VIN75_17325"
     CDS             complement(15854..16165)
                     /locus_tag="VIN75_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975981.1"
                     /translation="MRSLRTATFTISIRDSSEDELEAAAAAFTCTLHRAGMTPEDALL
                     AWRRMDEWERLNFAADADPGAQWWRTMAIARDAVLAALRAAGLDGQRRPFMIEPAARP
                     H"
     gene            complement(16192..16371)
                     /locus_tag="VIN75_17330"
     CDS             complement(16192..16371)
                     /locus_tag="VIN75_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8975982.1"
                     /translation="MIDDQPSEDIRSLFDWRSASHDGSEAKSSPVADDELALALSLLS
                     CNSRDREQFRGGRVA"
     gene            16507..16899
                     /locus_tag="VIN75_17335"
     CDS             16507..16899
                     /locus_tag="VIN75_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8975983.1"
                     /translation="MDRFHGGCLCGDVRFTATGRPYRVGVCHCLDCRKHHGALFHASA
                     IFPQDAVSLHGETHHFKGRHFCPRCGSSVFGRSGDEIEIHLGALDDIDRFRPTYELWT
                     IRREAWLPPFDVAHRHERDRAGPGRFED"
BASE COUNT         2332 a         5976 c         6198 g         2453 t
ORIGIN      
        1 gtctacggca gctacgcggc cggcgtgtac ctgacgccga tcgcgggcgg catgatctcc
       61 gaccgctggc tgggcaagcg gcgcgcggtg atcgtcggcg gcctgctgat ggcactcggc
      121 cacttcatga tggcctccga ggcgctgttc ttccccgcga tggtcgtgat cgcctgcggc
      181 aacggcctgt tcctgcccaa cctgcccagc caggtcaagc cgctctacgc cgcgggcgac
      241 ccgcggctgg cgggcgcgta caacctctac tacatgggca tcaacctcgg cggcttcctc
      301 gcgccgctgg tgtgcggcac gctgggcgag ctgtacggct ggcactgggg cttcggcgcg
      361 gcgggcgtgg gcatgtgcct gggcctggcc atctacgtgg cgggcgcgcg ccacctggcg
      421 cctgtcgacg cggccgacga ggccggcgcc gcgccggccg cgccggcagc ctcgcgccac
      481 cagatcctgt tcctggccgg caccgcgctg gcggtcgtga tctttcgggt cgcctacgcg
      541 cagaccggca acacgctggc gctgtgggcc gacaccgcgg tcgaccggcg cgccttcggc
      601 tggacgatcc cggccacctg ggtgcaactg ctcaacccgc tgtacgtgtt cctgatgacg
      661 ccgctgctgg tggcgttctg gaaccggcgc ggcgacgcgc aggcgcagtc ggcaccgctg
      721 tggaagatgg ccatcggcgc gatgacggtg gccgcgtcgt tcgcgctgct ggccgccgtc
      781 gcgcgcgcat ccgaggccgg cggcgcgccc gtgcactggt cgtggctggt ggccttcttc
      841 gcgctgctga cgctgggcga gctctacatc ctgccggtgg gcctgggcct gttcgcgcag
      901 ctcgccctgc caggcttcgg cgccaccacc atcgccgcgt ggttcctcgc cgcgttcgcg
      961 ggcaacctgc tgtccggcgt cgtgggcacc tggtggacgc acctgtcgcc cgacgcgttc
     1021 ttcctggcaa tgggcgcgct cgccgcgctc gccggcgttc tgctgacgct gctggccgcg
     1081 gccgagcgcc gctggttcac cgacctcatg acggcgcagc gcgccgggaa ccccccatga
     1141 cgacccctct cgcgcgccgc cgcgtcgact cgatgctggt gtgggacaac cacgcctgca
     1201 tgccgctcac cccgcacgac acgagcttcc tgccgcagtt gaagcgccac cgcgacgccg
     1261 gcgtcgacgt cgtgatggtc aacgtcggct tcggcgacga cggcgtcgag cagcacctgc
     1321 gcatgctcgc cgcgatgcgc gactggctgc tcgcccacgc gagcgagtac cggctgatcg
     1381 atacggtcga cgacatcgag atcgcgcgcg ccaacggcca gctcgcggtg gggttcgaca
     1441 tcgagggcgc caacgccatc gccgaccagc tgagcctgct cgggctgtat cgcgacctgg
     1501 gcgtgcgctg gatgctgctg gcctacaacc gcaacaaccg ggccggcggc ggctgccagg
     1561 acgacgacgc gggcctgtcc gcgttcggcc gccgcatgct gcacgagatg gcgcgcgtgg
     1621 gcatcgtggc ctgctgctcg catacgggat accgtacggc ccgcgaggcg atcgacgagt
     1681 cgccgacgcc ggtcatcttc tcgcactcga acgcccacgc actggtcgat cacccgcgca
     1741 acatccccga cgacctgatc aaggcctgcg ccgcgcgcgg cggggtggtg ggcgtcaacg
     1801 ggatcggcat cttcctgggc aacaacgacg cgtcggtggc caatgtcgca cgccacgtgc
     1861 tgcacatgct ggacctcgtc ggcccctcgc acgtcggcct gggcctggac tacatcttcg
     1921 acacccgcga cctcgccgcc gagatcgccg cccaccccga caagttcccg ccgcacctgg
     1981 gttattcggc cgacatcgcg atgaccgcgc cggagcaact gcccgacctg gccgacgagc
     2041 tggcgcgccg cggcgtcgac gacccgtcgc tcgcgctcat cttcggcggc aactggctgc
     2101 gcgtggcgcg cgaggtctgg cagccgccgt tctgagcggt gtcaggtctc cgtgccttgg
     2161 ggtcaggtct tgaaacttgc ggggtcaggt cttgagtgca cgattcaaga cctgaccccg
     2221 caagtttcaa gacctgaccc ctcgagcgag cagcggccgc acccgcgcgc cgaacgagaa
     2281 gtcccgcacc tgcccggtgc aggtgacgtg gcggcccagc gcggcgatgc gggcgacgac
     2341 gtcggcagcg aaatcctcgt tgtcgaagtc ggccagcacg acgccggtgc cgtcctggat
     2401 gtccagcgtg cgcacgagca gcgcggcgag cgcggccggg tcgtgcgtgg acgggttgca
     2461 gctcgtggcg cggtggtcgc ccatgccgtg cgcggcgatg acgatgctgt cgcggcccgc
     2521 gtcgaacgtc cgctggtcgc ccttgcgcga caccgagatc atcttggcgc gccggaagcg
     2581 cgcccagtgc gtggcgttgg cggccttctc ctcgtcgccg gtcagcgaga gcacgtacgt
     2641 ggtcatggca gtccttgcag cggtgggcgg gctcagttgg cggcgatctc ttcttcgcgg
     2701 gccaccgcgg ggcggccgcc ggcggcgatc acggcgccgc ccgcgcgcag cagctcgaag
     2761 ccgatctcgg ggcaggacgc ggccagttcc ttctggcggg ccagcgggag ctcgaggatc
     2821 agcgagtcct ccagggcgcg gacgtccagg tcgctggcgc cgtcggagaa gagcgcgtcc
     2881 tcgccgacga tcgtgccggc ggcgagcacc ttgtcgcggt tgccggcggc cgattcctgc
     2941 cacagggcgc cctcgacgac gaagcgcagg gtgcgatccg agcggccggg gatcatgacg
     3001 cggaagccgg ccggcacgcg gcgggcgacg cagaagctgg cgaacacgcg ccagtcggcg
     3061 ttggcgaagg cgggcaggaa ggcgtgggtc gaggtcgact tgcgcgccgc ggcggccagg
     3121 cgcaggccgt cgcagtcggc cggagcgggc acctggtcgc ggcacaggtc catggccagc
     3181 gggaactgca gcggcttggc ggcgggatag cggccgaagg cggtggcatt cgcggtgcgg
     3241 gtgtcggtgg gcgtgtggaa catggtggct ctcctcaact ggtcggtgtc ttcttgactt
     3301 gttcggtttg ccgaatcgct tcggcatggg acgcatgttc ggccggccgg gccgtgcgat
     3361 ccatcacggg agataacggc gggcgtgatg ccgggccgcc ctccgggttt gctccgggtc
     3421 ggcgcccggt gaaccgcgcc ggggcggggt gtccgccggc ccgggcgggt gacgcggggg
     3481 aactacactt ccggctgcgc cgaggcgtcc gtcggcgtgg agatttgcgc gagatgggta
     3541 cgttgttcga tccccgcacc ggcgaccgcg tggtgctccg cgccgaacac gtgttcggcc
     3601 gcaatgcgct gcgcgccgat accgccgtcg acgatccggg catctcctcg atgcacgccg
     3661 tcatccgctg gcgcaacggg cgctggatgg tgtccgacca cagcaccaac ggcaccttcg
     3721 tcaacggcca ggtgctggtg ccgggcgagc cgttcgcgct cgaggccggc gtcgagctgc
     3781 ggctgggccg cggcccgaac gtcgcatggc gggtgcagga cgtggccgcg ccggtggacg
     3841 tcctggtgcc ggccgacctg ctgcggccgg tgatcgtcct ggcggcgcac aacctgctcc
     3901 cgagcagcga cgcgccggag ctgtgcatct acctggcggg cccggggctg tggatgctgg
     3961 agcagaacgg cgagacgcgc cccctcaagg acggcgacgc cgtccagatg ggcgccggcg
     4021 cgcaccgctt cgtcgccgcg gcgccgatgg acgacacgca ggtggccgag gtggcccgcg
     4081 agccggagtc gccgctgctg gtgatgcgcc tgagcctgga cgaggagcac gccagcctgg
     4141 aggtcaagga cggcgtcgcc aggaccgacc tgggcgagcg cagccaccac tactgcctgg
     4201 cgacgctggc gcgccggcgc atggccgacc aggaacgcgg cctggaaccg ggcgcccagg
     4261 gctggctggc aagcgcggag ctgtcgcgca tgctgggcat ggagacgacg cacgtcaaca
     4321 tgcagatctt ccgggcgcgc gagcagctga tgacggcgct gccgggcgtg gccgcgctgt
     4381 cgcgcctgat cgagcgccgg cgcggcgagc tgcgcatcgg cgagctgccg ttcgagatct
     4441 accgcggcga ctcgctggag tgccgctacc ggccgctgcg ctgaggcggc gccagcgcgg
     4501 cttcgagctc cgcccgcaac gcggcctgct gcgacgcgga cgtgccggcc agcaggttgg
     4561 cgacgaacac gtcgagcgaa ccgccggcgg tgtcggccgc gcgccgcacc atcacgctgg
     4621 ccaccggccc cacgcggcgc gccagcagct gctgggcggt ggcgcaggct tcgggtggca
     4681 gcgtcgccgc gctcgcggcc gggacggcgg ccgaggtctc gtcggcgcct tcccggtggg
     4741 gaatgtcgac gatcgcatgg cacgagccgg cgtcgggtac gtagagcgcg aaggcgtcga
     4801 cccgctgcgc caggacgggc gcgtcgcgca gcaccacgcc cgccatcgga tcgagccggt
     4861 gcgctcgtgc atgctcgaag gcggcacgct gctcgggcga caggtggccg agcaggctgc
     4921 cggcgtcctg caccagggtg ccgaccaccg tgcccacgac ggtggccagc ggcgcgtcgg
     4981 cggcgtgcga cgcggccgcg tgcagggcgt cacgcatctc gtcggcgctc gcgaaacggt
     5041 cctcggcgcg cttggcgagc gcgcgggcga ccacgctgtc gtacgccggg tcggcgcggc
     5101 cgccgctggc ccggctggtc gggggcggct cctcgtgcag gatctgctgc atgacggcgg
     5161 ctggcgcgcc gcagaacggc gggcggccgg ccagcagctg gtgcagcagc gcgccgcagg
     5221 cgaacaggtc ggcgcggtgg tcgagcgcgc ggccggtgaa ctgctcgggc gccatgaacg
     5281 ccggcgtgcc gatgatcgac gacacgcggg tcagcccgag gtcgcgcagc cgcgcgacgc
     5341 cgaagtcgac cagcttcacg cgccctgcgg gcgtcagcag gacgttggcg ggcttgacgt
     5401 cgcggtgggt caggccttcg cgatgggcgg cgccgagggc gtccagcagc tcgtccagga
     5461 tggccagcgc tcgctcctcg gccagcggcg gcgtgccgcg cagcatgtcg gcgagcgtac
     5521 ggccctcgac gtggggcatc gcgagccagg tgttcaggcc gtcctcgccg atctcgtcga
     5581 tggcgacgag gccggggtgg tccagtcgcc gcacggcgag cgcctcgtcg cggaaccggc
     5641 gcaggatctc gtcggcgtcc ccgtcgctgt cggcgctgcg ctgcagcgtc ttgatcgcga
     5701 cggcccggcc gtcccgcggg tcgatgcccc tgtagacgat gctggtcgat ccccgcccga
     5761 tctcggcggt gatgtcgtag cggccaattc gttcgggaag catgacggga tgcgcgtggg
     5821 ggcggggtgc ctgccacgat tgtcgggcat gccggggagg tcttccttgg ggtcaggtct
     5881 tgaatgttgc ggggtcaggt cttgattcgt gcagcggcag gaaggtgtca ggtcttgaat
     5941 cgtgcactgc atgattcaag acctgacacc ggcttcggac accggctccg gctccgcgac
     6001 gccggctccg gatactggcc tccactttcg gaactgcacg attcaagacc tgaccccgca
     6061 acgttcaaga cctgaccccg caagagaccc gattcaagac ctgaccccgc aacattcaag
     6121 acctgacccc gcaagagacc ccgcaagatg actcggcaag aaggcaagtc ctgacaccac
     6181 caatgtcagg ccggccgcgc gaacggcgtg cggatgtccg ccaggaactg ctgcgcccgc
     6241 gggtgttgcg ggtggttgaa gaacgcgtgc ggctcggcgc gttccaggat gcggccctgg
     6301 tccatgaaca gcacgcggtg ggcgacgtcg cgcgcgaagc ccatctcgtg cgtgacgacg
     6361 accagcgtca tgccgtcgcg caccagccct tgcatgacga gcagcacctc gccgaccatc
     6421 tcggggtcga gggcgctggt gggctcgtcg aacagcatca gcggcggctc catcgccagc
     6481 gcccgcgcga tcgccacgcg ctgctgctgg ccgccggaca gctccgaggg ccacgcgttc
     6541 gccttgctcg cgaggccgac gcgatcgagc agcgccatcg ccttctcgtg cgcctgcgcg
     6601 ggccgcgcgc cgcccacctc gatcggcgcg agcgtgcagt tctgcagcac cgtcaggtgc
     6661 gggaacaggt tgaactgctg gaacacgaag ccgatgcgcg aacgcagccg gttcaggtcg
     6721 atgccggggc ggtggatgtc ctcgccgtcg aagctgatcg tgcccgactc gatggcctcg
     6781 agccggttga tcgtgcggat cagcgtggac ttgcccgagc ccgacggccc gcagacgacc
     6841 acgacctcgc ccttggcgat ggtctcgtcg atgtccacga gcgcatggta cgagccgtac
     6901 catttgttga ccttgctgaa ctggatcatg gattggcggc cgcggtggcc gtgggggagc
     6961 cgggcggatg gacggggcgc ggcaggtcgc ggcgcgcctg gcgccgctcg atcgcgagcg
     7021 ccacgcgcga cagcccgaag cacagggtga agtacatcag gctcagcagc aggtagaccg
     7081 cggtcggatg cacgaacacc tgcgagttga cctgcgtggc gatgaacgac gcctcggtca
     7141 ggccgatgat gtagcccagg gacgtcgcct tgatggtgga cacgaactgg ttgaccagcg
     7201 acggcagcat gctgcgcagc gcctgcggca ggatcacgcg gcgcatcgct cctccgtggc
     7261 ccaggcccag cgcccgcgcc gcctcgtact gtcccttggg cacgccccgg atgccggcgc
     7321 gcacgatctc gcccaggtag acgccgtcga acacgaccag cgcgatcagc atggtcgtga
     7381 actggtcggt cttgtgcccc gtgaccgtgg gcaggtagaa gtagatccag aacaccatca
     7441 gcagcagcgg gatgccgcgg atcaggtagg cgacgaccgt caccggccgg cgcagcgcgg
     7501 cgatgctgct ggtgcgcgcc aggcccatca gcaggccgat gggcatcgcc agcagcagcg
     7561 cgagcgcggc cagcagcacc gtcgcggcca cgccgcccag cggcccctgc gggtactggc
     7621 cgatgaggaa gtacacccag taggtctgga tcaggtcgat cacggcgccg gcctcacagc
     7681 gtgcgcaccg gccagcgccg gtggatccag tcggccgcgg ccgtcagcag cagggagccc
     7741 gcgaggtagg acacggtgac gaaggcgaag gcctcgacgc tgtggaacgc ggccgactcg
     7801 acgcgcgcgg cctggtacat catctcgccc acgccgatga cggtggccac gctcgtgtcg
     7861 ttccacagga tgatgacctg cgacagcagc ggcggcaccg tcacgcgcag cgcctgcggc
     7921 agcacgacgc ggcgcatcgc agggatgaag cccagcccca gcgcgcgcgc cgcctcgaac
     7981 tgcacggcgg ggatcgcgcg gatgccgctg cggatgttct cggccatgaa cgccgcggcg
     8041 tacagcgtca ggccgatggc cgcggcgatg aattcgtagc tgccggcgta cagccgctcc
     8101 ttccacgcat cgggcagcag ctcgggcgcg ccgaagtacc agaacagcag gtgcgcgatc
     8161 agcggcacgt tgcggatgcc ttcgacgtag gtcgcgccca gccactgcag cggcggccag
     8221 ggcgacagcc gcagcagtgc gatcgcgatc gccagcggca gcgcgcaggc gatcgtgacg
     8281 ccggcgagct tcagcgagac gagcaggccg gcgagcagcc actcgtggta ctggccatgc
     8341 agcagcagct ggaggtcgaa gccgggcact cagatcttgt cggagtcgat cttgaacgag
     8401 cgcttgtcga actgcagctt cgtggtgggg ccgtaccact tcatgaagat cttgtcggcc
     8461 tcgcccgact tctcgagctc ggccagcgtg tcgtcgacca ccttcttcag cgcgggctcg
     8521 ttcttgcgga tgcccagcgc caggggctcg gtgctgaggt tggtcggcag gatgcggtag
     8581 tccttcctgg ccgggcccag cttggccatg tcgttgacca gcgtggcctc gtcgttgacg
     8641 tagccggcgc ccttgccctg ctgcagcgcc tggaaggcct gctgcgtggt ctcgaacgtg
     8701 acgacgtcga cgctgggcac cgccttgcgc acgttctcct cctgcgtgcc gcccttgacg
     8761 gtcagcacct tcttgcccgc gagctggggc acgtcggtga tgccgctgct ggccttgacc
     8821 atcaccttct ggcccgtgac gaaggtcgtc agcgagaagt cgatctgcga ctcgcgctcc
     8881 ttgttgtgcg tgagcgaggc ggccagcagg tcgacgcggc cctgctgcag ctcggggatg
     8941 cgcgcggcca cggcgatctg cttgaacgtc gccttgacgc cgatcttccg ggcgacggcg
     9001 ttggcgaggt cgacctcgta gccgacgatc tcgcgcgtct tcgggtcgac gaagctgttg
     9061 ggctcgtcgg tgccgaggac gccgacgacg atctcgccct tcttcttgat gtcggccagc
     9121 tggtcggcat gcgcggcgga cacgagggtc gtcgcggcga ggacggcggc gagcagggag
     9181 cgcaggttgg ttcgcatagg cttcgcggtt tgttgtcgag tgggcgggag gttacgggcc
     9241 gcgcgggacg ccgcgaagcg ccggaaacgc atatcctact gcgacggcga tgaccgtgct
     9301 caggccagct cgagatgctc gggcgagagc gcgcgcagga aatccgcggc gtccacggcc
     9361 tgccccagcg cgatcgcgcc cgagcgcgca aaggccggcg ccatcaggcg ttgcgcggcc
     9421 tgcaccacca gcggcgcgga cacggcgtag atgtcctggc cgcgcgccgt cgcctggcgc
     9481 acccggtcgc cctggcgtgt gcgcaccacc atctcgaagc gctgcggcga acggccgagg
     9541 gcgtcggtcg cggccggcgg cggcgtcgtg gtgtcgcgga tctcgtccag cgacgactgc
     9601 gtcaggtgcg cgctcatcgc acccaggcgc aggtggcgcg tcagggtgac gacctcgctg
     9661 aacgtgagct cgacgacctc ctgcttcccg tgcggcgcgc cgaacgtcca cgtcgagcgc
     9721 gcggccggca ggggcagcgg cgcgaggcgg ccgtcggcca cgaccagccg cggaaaggtg
     9781 ttgcgctcgc cggtgacgcg cgtgccggag gtcggccacc agcggtccag ggccacggcc
     9841 acggtgagct cgtcggccgc gccgtcggcg cccagcagcg atgtggccag caggtcggcc
     9901 aggccgccat agaagccggc ggccgggatg acggcgacgc cggcggcacg cgcggccgcc
     9961 tcgaagcgct cgaaggtgtc catcgcgctg gcctgctcgg cggtcacgtc caggtagtgg
    10021 gcgccgccgc gcagggcggc ctcgatgacg ggggcggcgg tgtccaggaa cgggccggcg
    10081 cagttgatga cgacggcgca gccggcgaag gcggcgtcga gcgcggccgg gtcgtcgatg
    10141 gccgcgtcgc gggcgggcac gtccgtcggc agcttggcgg cgttgcggcc gaccgcgatc
    10201 gcggccaggc cgcggcgcct gagctcggcc agcacgaagc gggcggtgtg gccggcggcg
    10261 ccgtagacgg caacgggacg gagggcggac gaagcggtca cggcgggttc cctttcattt
    10321 gaaacagaca ggtctgtatc gtttagaata aagacaggtc tgtatcatgt caacatgccc
    10381 agcaagaaat cctccggtgg cgacgccgcc ggcgagtcct ccgacgtccg tcaacgcatc
    10441 ctcgacaccg cctcccggct gttctacgag cgcggcgtgc gcgccgtggg cgtcgacctc
    10501 gtggtggacg actccggcgt cgccaagacc agcctgtacc gccacttccg caccaaggac
    10561 gacctgatcg tggccttcct ccaacgcgag gacgccgagt tctgggccca gtgggacgag
    10621 gtgcatgcgc gccacgcggg cgagccgatg gccgagctcg aggcgcacgt gcgctggatc
    10681 ggcaagcggc tggcgcgcgc caactaccgc ggctgcccgc agatcaacgt ggccgccgaa
    10741 ttcgccgagg ccgaccatcc gtcacgcgcc gtctcgcgcg agcacatgca ggcgatgcgc
    10801 aagcggctgg ccgagttggc gcgcgggctc ggcgccgcgc ggccgggcga gctcgcggcg
    10861 cagctgggtg tgctggtcaa cggtgcgttc gtcagcgcgg ccatgctctc gcccgaggag
    10921 gcgacgcgcg tgctgctcgg cgcggcgcgc gcgctggtgg cggcttcgcg ttgagcgcgg
    10981 gctgcgacag aatcgcgcga tgaccgatca ccacgcctcg tgttcctgcg gccagctgag
    11041 gctggtggcc aagggcgacc ccgtccgggt gtcgatgtgc cactgctacg cctgccagcg
    11101 ccgcaccggc agcacctacg gtgcacaggc gcgcttcctc accgagaacg tccggatcac
    11161 gggcacgtcc accgagtacg tgcgcctggg cgagtccggc gcggcggtgc acttccactt
    11221 ctgcccggac tgcggcgcga ccgtctacta ctggttcgac gacacgttcc gggccgtgcc
    11281 cgtcggcgcc ttcgccgaca acaccttccc gccgccgcgc atctcagtgt gggaggaccg
    11341 catgcacccc tgggtgagcg tgcccgaggg cgcggagcgc atggcatgag cgcccgcccg
    11401 gccgctccga agggcgctcg caccgcagcc cgcagggcgg aggttgcctc atgagcgacg
    11461 cggcgcccgg gccctgggtg ctgtacctga tcgaatgcac cggcgaacgc ctgtacgccg
    11521 gcatcaccaa cgacctcgcc gcacgtttcg ccgcccatcg agcgggtcgc ggcgcgcgct
    11581 tcacgaaggc gttcgcgccg ctgcgcgtgg tcgccgcgtc gcagctgccg tcgcgctcgg
    11641 aagccttgaa ggccgagcac gcgctcaagc gccagccgcg cgcggccaag ctcgcgttcc
    11701 tggccgcctg cggcacgccg ctggcgccgt ccgctctgcc gcaggactga tcgagaaccg
    11761 cacgatccgg ccgccgtgct gtcccaggcc ggcacagcgc cggcgtcgcc gcgtcgtccg
    11821 ccccgggcgc cgcccggcgc cctcgactgg aacgccattt gcatccgcca cccgcaacgg
    11881 atcatgatgg agggatgatg acccaggcgt tccattcgat gcgctggcac gtgccggcgg
    11941 cggccgtcgc ggccgcgggc agcgccctgt gcgccgcgca gcagcccgac gcggagccgc
    12001 agacggtcac gatcaccgcc acgcggatcg ctacgccggc gttcgacgtc cccgcgtcga
    12061 tctcgctggt gaccctgtcg cccgacactg actcgcgccc cggcatcaac ctgtccgaga
    12121 cgctggccgg cgttcccggc ctgctggcgc gcgaccgcca gaactacgcg caggacgtgc
    12181 agatctcggt gcgcggcttc ggcgcgcgct cgaccttcgg catccgtggc gtgcggctgt
    12241 atgtcgacgg catccccgcg acgatgcccg acgggcaagg gcagatctcc aacgtcgacc
    12301 tcggctcggc ggcggccgtc gaggtgctgc gcgggccgtt ctcggcgctg tatggcaact
    12361 cgtcgggggg cgtcgtgcag gtgttcaccg aggacggcgc cggtgcgccc aggctgaccg
    12421 tgggcgccgg cggcggcagc gatggccagc ggcgctacga cgccaagctc tcgggcgcgc
    12481 aggggccgct ggactacgtg gccagtgtgt cgcgcttcga gaccgacggc taccgagagc
    12541 acagcgccgc gcggcgcgac atccagaacc tgaaggcgag gctgcagctg ggcgatgccg
    12601 gcaagctgac gatcatcggc aatggcgtgc aactgccgtc cgcccaggat ccgctgggcc
    12661 tgacgcgcgc cgagctgcag gccgacccgc gcggcgtcga ccccgcggcc acgcagttcg
    12721 acacgcgcaa gtcgctttcg cagacccagg gcggcctcat ctacgagctg cgcgtgaatg
    12781 acgcgaacgc gctgcgggcg atggtctacg acggccatcg cgacaccacg cagttccagt
    12841 ccatcccggt cgccacgcag gccaacccgc tgaacccggg cggagtgatc cggttgggcc
    12901 gcgactacga cggcgccgac ctgcgctgga cgatcaccgg cggcccggcc gagcggcgct
    12961 ggtcgctgat cggcggcgcc gcctgggaca agctgcgcga acaccgccag ggcttccagg
    13021 acttcaccgg cgaccccgcc gccccggtgt caggcgtgca gggcgcgctg cggcgcgacg
    13081 agatcaacga cgtcgaggac ctggaccctt acgtgcaagg ccagtggcat ccctccgagg
    13141 actggacgct ggacgccggc gtgcgccgca gccgcgtgcg cttcgtctcg cacgatcgct
    13201 acgtcgtggg cgccaacccc gacgacagcg gccaggcgac ctacgaggcg acgctgcccg
    13261 tgctgggcgt gcagtgggcc gcgagtcccg agctgcacct gtacgcgacg gccggccgcg
    13321 gcttcgagac gcccacgctc aacgagctcg cctaccgccc cagcggcctg accggcctga
    13381 acttcgacct gaagccggcg cgcagcgaga gcctggagat cggcgccaag gcgcattcgg
    13441 cgtcgctggg cgtgctcgac gtcgcggtct tcggcacgcg cactcgcgac gagatcgtga
    13501 cgctgtccaa cgtcggcggg cgttcgacct accagaacgt cgcgtccacg cggcgcctcg
    13561 ggctggaggt ggcgtggacg aagctgctgc ccgacgacgt ggcgctgcag gtcgcctaca
    13621 cgctgctcgc cgcgcagtac accgacggct tcctcgcgtg cacgacgacg ccgtgcaccg
    13681 cgccggcgac accggtcgcg tcgggcaacc gcatcccggg cgtcgcgcgc aacgcgctgt
    13741 ccgcggagct gggctggacg ccgccgcgcg gctggcgcgc cagcgtgggg gcgcgggcgg
    13801 tgggccgcgt cttcgtcaac gacacgaatt ccgattccgc ttcgtcgttc gtgacggcga
    13861 acgtgtcggc gggctacgtg ctgccggtgg gggcgtggaa tttctcggcg ttcgcgcgcg
    13921 tggacaacgt gacggaccgg cgttacgtcg gttcggtgat cgtcaatgaa ggcaacgggc
    13981 gctacttcga gccggcgccg gggcgcacgt tcttcgcggg gctgactacg agctatcggt
    14041 tctgatggcg ggatcggatt cgcatgggca tgttcgatca cagcgtgccg ggatacgccc
    14101 cgccatcgat cagcacgttc tgcgccgtga tgtaccccgc ctgcgcgctg cacaggaacg
    14161 cgcaggccgc gccgaactcc tcgggcgtgc cgaagcgccc ggccgggatc gcggcctcgc
    14221 gctgccgcgc gacctcgtcg gtgccgatgc ccaggcgctg cgcgcgcggc gcgaacgatg
    14281 acagcaggcg gtcggtggcg aaggcgccgg gcagcaggtt gttgatcgtc acgccgcgtc
    14341 cggccacctc gcgcgcgacg cccgcgacga agccggtcag gcccgagcgc gcgccgttgg
    14401 acaggctcag cgacgcgatc ggcgacttca ccgacgagct cgtcacgttg acgatgcgcc
    14461 cccagccgcg cgcggccatg ccgtccacgg tcgccttgat cagctcgatg ggcgtgagca
    14521 tgttggcctc gaccgcctcc agccaggcgg cgcggtcgaa gtcgcggaag tcgcccatcg
    14581 gcgggccgcc ggcgttggtg acgacgatgt cgaacgccgc cggcgcgccg tggcgcagct
    14641 cgcgcgcggc gtcgagcgcg agcgcgcggc cggcgtcggt ggtgatgtcg gccgcgatcg
    14701 cgacgacctt gacgcccgcc gcctcgcgca gggcctgcgc ggtcgcctcg agcgcgtcac
    14761 gcccgcgcgc gacgagcacc aggtcgacgc cctcggccgc cagcgcgccc gcgcagccgc
    14821 ggcccaggcc cttgctcgac gcgcacacga gcgcggcctt gcccttcaat ccgagatcca
    14881 tgtcattgcc ttgtgtggtc gtggccttgg cggatcgtat atcttgcgcg ttaccatgac
    14941 ggacgccccc aagagactgt ccatcgacgc cggcgtgctg cgcgtcgcct gcctcgaata
    15001 cggcgaaccg gccggtcgcc cggtggtgct gctgcacgga ttcccctacg acgtgcacgc
    15061 ctacgacgcc gtcgcgccgc ggcttgcggc gcgcggcatg cgcgtcatcg ttccgtacct
    15121 gcgcggctac ggcccgacgc gcttcctgtc cgacgcgacg ccgcgctcgg gccagcaggc
    15181 cgcgctcggc gccgacctgc tggcgctgct ggacgggctg tccctcgagc gcgcactcgt
    15241 cggcggctac gactggggcg gccgcgcggc ctgcatcgtc gcggcgctct ggcccgggcg
    15301 ggtacggggc ctggtatcgg ccaacggcta caacctgcag gacatcgcgc gttcggtcga
    15361 gcccgccacg cccgacaagg agtaccggct ctggtaccag tactacctgc acggcgagcg
    15421 cggacgcgcc ggcctggccg cgcaccggcg cgagttcgcg cggctgctgt ggcagttgtg
    15481 gtcgccgtgc tggaagttcg gcgacgccga cttcgagcgc accgcgccgg cgttcgacaa
    15541 ccccgacttc gtcgacgtcg tcgtccattc gtaccgtcac cgcttcgggc tggtcgacgg
    15601 cgatccggcg ttcgagggca tcgagcgccg cctcgccgcg cagcccgcca tcgcggtgcc
    15661 cgcgatcacg ctggacggcg acgccgacgg cgtcacgccc gacggcacgc ccggtcccgg
    15721 ccggttcatc ggccggcacg aacaccggcg gctggaaggc ggcgtcggcc acgacttgcc
    15781 gcaggaagcg ccgcaagcct ttgcgcaggc ggtgctggac gtggacggct gggccgcggg
    15841 ctgacgcggc gattcagtgc ggccgcgcgg ccggctcgat catgaagggc cgccgctggc
    15901 cgtcgagccc ggcggcgcgc agcgcggcca gcacggcgtc gcgcgcgatg gccatcgtgc
    15961 gccaccactg cgcgccgggg tcggcgtcgg ccgcgaagtt caggcgttcc cactcgtcca
    16021 tgcgacgcca ggcgagcagg gcgtcctcgg gcgtcatgcc ggcgcggtgc agcgtgcagg
    16081 tgaacgccgc ggcggccgcc tcgagctcgt cctcggacga gtcgcggatc gagatcgtga
    16141 acgtggccgt gcgcagcgag cgcatggccg aatccgtggt ccgcatgccc gtcaggccac
    16201 ccggccgccc cggaactgct cgcggtcgcg agaattgcac gacagcaggc tgagcgccag
    16261 cgcgagttcg tcgtcggcca ccggcgaact ctttgcctcg ctcccgtcgt gcgacgcact
    16321 gcgccagtcg aacagggaac gaatgtcttc gctgggttgg tcgtcgatca cggcgtggcc
    16381 ctttctgtcg tcgaggttgc ggttcggggg atcggcaagc ggcgtgcctt gcggctcacg
    16441 gggccattgc cagggcgctg cccggatgca accatgccgg tcatccgatc tgcaaggaag
    16501 cgcgacatgg accgattcca cggcggttgc ctctgcggcg acgtacgctt cacggccacc
    16561 ggccgcccgt accgggtggg cgtgtgccac tgcctggatt gccgcaagca ccatggcgcg
    16621 ctgttccacg cgtcggcgat ctttccgcag gacgcggtga gcctgcacgg cgagacgcat
    16681 cacttcaagg ggcgccactt ctgcccgcgc tgcggctcgt cggtcttcgg ccgcagcggc
    16741 gacgagatcg agatccacct cggcgcgctg gacgacatcg accggttcag gccgacctac
    16801 gagctctgga cgatccgccg cgaggcgtgg cttccgccgt tcgacgtggc gcatcgccac
    16861 gagcgggacc gcgcgggccc gggccggttc gaggactagg agtctgcgcg gcagaagccg
    16921 cctactgcga acggcgatga gcggggcctt tccagcggc
//
LOCUS       DATCRO010000634         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_693574, whole genome shotgun sequence.
ACCESSION   DATCRO010000634 DATCRO010000000
VERSION     DATCRO010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693574"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1091)
                     /gene="glk"
                     /locus_tag="VIN75_17340"
     CDS             complement(111..1091)
                     /gene="glk"
                     /locus_tag="VIN75_17340"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY8975984.1"
                     /translation="MTAANTSSFPRLMGDVGGTNARFALQEAPGAQPSQVRTYAVAEH
                     ANIDGAMKAYLDQVAGPRPRQGAVGIANPIVGDFLRMTNSPWAFSIEALRVTLGLDRL
                     LFINDFTALALSLPSLQPEHLRRIGTAGTADPKAAIGLLGPGTGLGVSGLLYDTAGHL
                     VPLGGEGGHVSLASANAREDAVVAALRERFGHASAERALQGAGLVAMYEALCKIDGVP
                     AATLDPAGVTTAAMAGSDARCVEVIELFFAFLGSVAGNLALTLGARGGVYIGGGIVPR
                     LGDWIDRSTFRERFIAKGRFRGYLDAIPTWLIHAETSPALIGAARALDEL"
     gene            complement(1292..3190)
                     /locus_tag="VIN75_17345"
     CDS             complement(1292..3190)
                     /locus_tag="VIN75_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEY8975985.1"
                     /translation="MHHPRKLSAAVLLAASSLGASAAAPDVPPMPAQIERIEPAFWWV
                     GMKSDKLQLLVHGPRIADAAPQIGDASAASVRIESVSRVANPNYLFIDLRIAPDAAPG
                     RVDIVFSHGDERFHAPFELRERAPGSAQRRGFDSSDVILNLVPDRFANGNPANDNVPG
                     YGDPANRADDEAGRHGGDIQGIVDHLDYFRDLGVTMLWPTPLTENRQPRYSYHGYAAT
                     DTYKIDPRFGTNEDYRRMVESARAKGMGVIMDVVLNHVGSEHWWMHDMPAPDWIGFDG
                     KFVQTQHARTTASDPYASRADARIYTDGWFGPNMPDMNQRNPFLATYQIQNAIWWVEY
                     AGLSGLRVDTWGYSDQAFLQEWTRRVMQEYPNLNIVGEEWTSQPTVLMHWLGGGRDAR
                     GTPLPSAMDFPLNGTLRKALASDTDDLHAGLMDLYEGLVNDVLYPQPGKMVLFEGNHD
                     LPRLYSVLKEDVGLTKMALAYVMTMPRVPQLYYGTEVLMTSPTVRDDGKARQDFPGGW
                     AGDKVNAFTGKGLSARQKDMQAFVRTLLNWRKTQSTIHHGKLMHFVPENGTYAWFRYD
                     ADSTVMVVINKNQAETTLDLGRFAEVLKTPATARDVLAGKQVKLGGTVKLPARSVKIF
                     ELRAESLR"
     gene            complement(3202..>3561)
                     /locus_tag="VIN75_17350"
     CDS             complement(3202..>3561)
                     /locus_tag="VIN75_17350"
                     /inference="COORDINATES: protein motif:HMM:NF023280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEY8975986.1"
                     /translation="LLRIRASTPLFHLPTAEEVRRRISFPASGPSQEPTVVAELVDGR
                     GLAGAKFGAVLVVLNVDKQSHALDLPALKGRRWRLHPVHLAAHAADTRIAKDAKVDDA
                     SGRFTVPARAAVVFVAR"
BASE COUNT          507 a         1226 c         1274 g          554 t
ORIGIN      
        1 ggcgtaaccc gcgatccggc agcaccgtgg cacgtccacc cggcgggtta cgcctgcggc
       61 taacccgccc taccacatcc cgtcgctacc gccgctgccg gcccgccggg tcagagctcg
      121 tccagcgccc gcgccgcgcc gatcagcgcc ggcgacgtct cggcatggat cagccacgtc
      181 gggatcgcat ccaggtagcc ccggaaccgt cccttggcga tgaagcgctc gcggaacgtc
      241 gagcggtcga tccagtcgcc caggcgcggc acgatgcccc cgccgatgta gacgccgccc
      301 cgcgcgccca gcgtcagcgc caggttgccc gccacgctgc ccaggaacgc gaagaacagc
      361 tcgatcacct cgacgcagcg cgcgtcgctg cccgccatcg ccgcggtcgt gacgccggcc
      421 gggtcgagcg tggcggccgg cacgccgtcg atcttgcaca gcgcctcgta catggccacc
      481 aggcccgcac cctgcagcgc gcgctcggcc gacgcgtgcc cgaaacgctc gcgcagcgca
      541 gcgaccaccg cgtcctcgcg cgcattggcc gacgccaggc tgacgtggcc gccctcgccg
      601 cccagcggca ccaggtggcc ggcggtgtcg taaagcaggc ccgagacgcc caggcccgtg
      661 cccgggccca gcaggccgat ggcggccttg gggtcggcgg tgcccgccgt gccgattcgg
      721 cgcaggtgct cgggctgcag cgacggcagc gagagcgcga gcgcggtgaa gtcgttgatg
      781 aacagcagcc ggtccaggcc cagcgtgacg cgcagcgcct cgatggagaa ggcccacggc
      841 gaattggtca tgcgcaggaa gtcgccgacg atcgggttgg cgatgcccac ggcgccctgg
      901 cgcggcctgg gccccgccac ctggtcgagg tacgccttca tggcgccgtc gatgttcgcg
      961 tgctccgcga ccgcatacgt cctgacctgc gacggctggg cgccgggcgc ttcctgcagg
     1021 gcgaagcggg cgttggtgcc gccgacgtcg cccatcaggc gcgggaagct ggaggtgttg
     1081 gcagccgtca tggaccggag gatctccctg gaatggcagg atggcgcggg caaagcgctg
     1141 cccgcattcg cgacaaaagt cgtgaatgta gtttacctac gattccagat caactgcggc
     1201 gcatcaaata ccgggctccg ggcacgccgt cgccacgacg ggtccgaagc tgaaagccgc
     1261 ggtcgtagtc tgcatacacc tttacgctcg attaacgcaa gctttccgcg cgcagttcga
     1321 agatcttcac cgagcgcgcc ggcagcttga ccgtgccgcc gagcttgacc tgcttgccgg
     1381 ccagcacgtc gcgcgcggtc gccggcgtct tcagcacctc ggcgaagcgc cccaggtcca
     1441 gcgtcgtctc ggcctggttc ttgttgatca cgaccatcac cgtcgagtcg gcgtcgtagc
     1501 ggaaccaggc gtaggtgccg ttctcgggca cgaagtgcat cagcttgccg tggtggatcg
     1561 tggactgcgt cttgcgccag ttcagcagcg tgcgcacgaa ggcctgcatg tccttctgcc
     1621 gggcgctgag gcccttgccg gtgaacgcgt tcaccttgtc gccggcccag ccgcccggga
     1681 aatcctggcg cgccttgccg tcgtcccgga cggtgggact ggtcatgagc acctcggtgc
     1741 cgtagtacag ctgcggcacg cgcggcatcg tcatcacgta cgccagcgcc atcttcgtca
     1801 ggcccacgtc ctccttgagc acgctgtaca ggcgcggcag gtcgtggttg ccctcgaaca
     1861 gcaccatctt gccgggctgg ggatagagca cgtcgttgac cagcccctcg tacaggtcca
     1921 tcaggcccgc gtgcaggtcg tcggtgtcgc tggccagcgc cttgcgcagc gtgccgttca
     1981 gcgggaagtc catcgcgctg ggcaacggcg tgcccctggc gtcgcgcccg ccgcccagcc
     2041 agtgcatcag caccgtgggc tgcgacgtcc actcctcgcc gacgatgttc aggttcgggt
     2101 actcctgcat cacgcggcgc gtccattcct gcaggaaggc ctggtcggag tagccccagg
     2161 tgtccacgcg caggcccgac agcccggcgt actcgaccca ccagatcgcg ttctggatct
     2221 ggtacgtggc caggaacggg ttgcgctggt tcatgtcggg catgttcgga ccgaaccagc
     2281 cgtcggtgta gatccgggcg tcggcccgcg acgcatacgg gtcgctcgcg gtcgtgcgcg
     2341 cgtgctgcgt ctgcacgaac ttgccgtcga agccgatcca gtcgggcgcg ggcatgtcgt
     2401 gcatccacca gtgctcggag ccgacgtggt tcagcaccac gtccatgatc acgcccatgc
     2461 ccttggcgcg cgcggactcc accatgcggc ggtagtcctc gttcgtgccg aagcgcgggt
     2521 cgatcttgta ggtgtcggtg gcggcgtagc cgtggtacga gtagcgcggc tgcctgttct
     2581 cggtcagcgg cgtgggccac agcatggtca cgccgaggtc gcggaagtag tcgaggtggt
     2641 cgacgatgcc ctggatgtcg ccgccgtggc ggccggcctc gtcgtcggcg cggttggccg
     2701 ggtcgccgta gcctggcacg ttgtcattgg ccgggttgcc gttggcgaag cggtcgggca
     2761 ccaggttcag gatgacgtcg gacgaatcga agccgcggcg ctgggccgag ccgggcgcgc
     2821 gctcgcgcag ctcgaacggc gcgtggaagc gctcgtcgcc atggctgaag acgatgtcga
     2881 cgcgcccggg cgccgcgtcg ggcgcgatgc gcaggtcgat gaacaggtag ttcggattgg
     2941 ccacgcgcga gaccgactcg atgcgcacgc ttgcggcgga cgcgtcgccg atctgcggcg
     3001 cggcgtcggc gatgcgcggc ccgtgcacga gcagctgcag cttgtcgctc ttcatgccca
     3061 cccaccagaa cgcgggttcg atgcgctcga tctgcgcggg catgggcggc acgtcgggcg
     3121 ccgcggcgct cgccccgagg gacgaggcgg cgagcaggac ggcggcggag agtttgcggg
     3181 gatggtgcat gcggggctct ttcagcgcgc gacgaagacg acggcggcgc gcgcggggac
     3241 ggtgaatcgg ccactcgcgt cgtcgacctt ggcatccttg gcgatgcggg tgtcggcggc
     3301 gtgcgcggcc aggtggacgg gatgcaggcg ccagcggcgt cctttcagtg cgggcaggtc
     3361 cagcgcatgc gattgcttgt cgacgttgag gacgaccagc accgcgccga acttcgcacc
     3421 cgcgaggccg cggccgtcga ccagctcggc gacgacggtg ggctcctgcg acggcccgct
     3481 ggccgggaag ctgatgcggc ggcgcacctc ctcggcggtg ggcagatgga acagcggcgt
     3541 gctggcgcgg atgcgcagca g
//
LOCUS       DATCRO010000635         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_333629, whole genome shotgun sequence.
ACCESSION   DATCRO010000635 DATCRO010000000
VERSION     DATCRO010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333629"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VIN75_17355"
     CDS             <1..1434
                     /locus_tag="VIN75_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8975987.1"
                     /translation="ALGARAARRPVKVALPRPLMANNTTHRPATIQRIRLGAGRDGRL
                     TAIAHEGWSGDLPGGQPETAVNQTRLLYAGANRLTTTRLAVLDLPEGNAMRAPGEAPG
                     MMALEIAMDEMAEKLGIDPVEFRVLNDTQVDPEKPQRPYSQRKLIECLRTGAERFGWN
                     RRNPAPGQQRDGRWLVGMGVAAAFRNNLVTKSGARVRLDNRGMVTVETDMTDIGTGTY
                     TIIAQTAAETMGVPLDKVLVRLGDSAHPVSAGSGGQWGANSSTSGVFAACMKLREAVA
                     SRLGLPAAEAEFADGMVRAGSRAVPLAEAAGAQGLVVEDSIEFGDLDKKYQQSTFGAH
                     FVEVGVDAATGEIRVRRMLAVCAAGRILNPKSARSQVIGAMTMGVGGALMEELALDKR
                     HGFFVNHDLAGYEVPVHADIPHQDVVFLDETDPISSPMKAKGVGELGMCGVAAAVANA
                     VYNATGVRVRDYPITLDKLLDRLPPVA"
     gene            1543..2586
                     /locus_tag="VIN75_17360"
     CDS             1543..2586
                     /locus_tag="VIN75_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8975988.1"
                     /translation="MDSSISSSAAHGAHGADVALYRAMVRIRAFENAAETASQGGVSA
                     YGQQAAGAAKVRGPLHLSTGQEAVPAGVCAHLRPADYLTSTHRGHGHTLAKGADLARM
                     MCELFGKATGFNGGKGGSMHIADFSVGMLGANGVVAAGLPIAVGAAHAQKLLKKSDDI
                     TVCFFGDGAINRGPFLEALNWARVYELPVLFVCEDNRWSATTASGPMTAGEGASARAE
                     SLGIASTQVDGNDVFAVHAAAAKLVAEVRTGGGPRLLHALTYRVKGHVSVDVAAYRDP
                     AELAAALETDPIARARGHLLSAGVATATLDAIENAARDEVDTALAVADAAPWPEPAAA
                     FTDVQTVGEGQWR"
     gene            2583..3542
                     /locus_tag="VIN75_17365"
     CDS             2583..3542
                     /locus_tag="VIN75_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8975989.1"
                     /translation="MKHELSYSEAALLALQREMEADPRVVVLGEDVGRGGIFGQYKGL
                     QQSFGPERVIDTPISEAAIMGAGVGMALAGLRPVVEMRVVDFALCGMDELVNQAAKNR
                     FMFGGQGRVPLVARMPGGIWDASAAQHSQSLEAWFAHLPGVVVVSPSTPQDNHGLLRS
                     ALQCGDPVVYIEHKTLWGARGEVDEGIAVPLGKAARVREGDALTLVSWSRQMQACSAA
                     CDALAAEGIAADLIDLRTLWPWDRETVLSSCARTGRLLVVHEAVQAAGFGAEIAASAA
                     EATGCRVARLGAPRIPVGYAPVLEAQSRVGTEAIVAAAKKLVG"
     gene            3567..3992
                     /locus_tag="VIN75_17370"
     CDS             3567..3992
                     /locus_tag="VIN75_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8975990.1"
                     /translation="MDPLFFKLVRVVNLTARPFHERVGREHRLTLNEWRTMAVLAAQP
                     GLNATQVADQTGLDKMAVSRALAGLLRHKRVQRHDDPTDRRRSRLYLTAAGKALHETV
                     GALGREREVALFGGIGAVELAQLDTTLDKLIEAVQRGTP"
BASE COUNT          603 a         1403 c         1507 g          517 t
ORIGIN      
        1 gcgctgggcg cgcgggctgc gcggcggccg gtgaaggtcg cgctgccgcg gccgctgatg
       61 gcgaacaaca ccacgcaccg gcccgcgacc atccagcgca tccgcctggg cgccggccgg
      121 gacggcaggc tcaccgccat cgcgcacgaa ggctggtcgg gcgacctgcc gggcggccag
      181 ccggagaccg ccgtgaacca gacccggctg ctgtacgccg gcgcgaaccg cctcacgacc
      241 acgcgcctgg ccgtgctcga cctgcccgag ggcaacgcga tgcgcgcacc cggcgaggcg
      301 cccggcatga tggcgctgga gatcgccatg gacgagatgg ccgagaagct gggcatcgat
      361 ccggtcgagt tccgcgtgct caacgacacg caggtcgatc ccgagaaacc gcagcgcccc
      421 tactcccagc gcaagctgat cgaatgcctg cgcaccggcg ccgagcgctt cggctggaac
      481 aggcgcaacc cggcgccggg gcagcagcgc gacggccgct ggctcgtcgg catgggcgtg
      541 gcggccgcgt tccgcaacaa cctggtcacg aagtcgggcg cgcgcgtgcg cctggacaac
      601 cgcggcatgg tcacggtgga gaccgacatg acggacatcg gcaccgggac ctacaccatc
      661 atcgcgcaga ccgccgccga gaccatgggc gtgccgctcg acaaggtgct ggtgcgcctg
      721 ggcgactcgg ctcacccggt ctcggccggc tccggcgggc aatggggcgc caacagctcg
      781 acctcgggcg tcttcgccgc atgcatgaag ctgcgcgaag cggtcgcctc caggctgggc
      841 cttccggcgg ccgaggcgga gttcgcggac ggcatggtgc gcgccggctc gcgcgcggtg
      901 ccgctggccg aagcggccgg cgcgcagggg ctggtggtgg aggacagcat cgaattcggc
      961 gacctcgaca agaagtacca gcagtcgacc ttcggcgcgc atttcgtgga agtcggcgtc
     1021 gacgcggcca ccggcgagat ccgcgtgcgg cgcatgctcg cggtgtgcgc cgcggggcgc
     1081 atcctgaacc cgaagtccgc gcgcagccag gtgatcggcg cgatgacgat gggcgtgggc
     1141 ggcgcgctga tggaggagct cgcgctcgac aagcggcacg gcttcttcgt caaccacgac
     1201 ctggcgggct acgaggtgcc ggtgcacgcg gacattccgc accaggacgt cgtcttcctc
     1261 gacgagaccg acccgatctc ctcgccgatg aaggccaagg gcgtcggcga gctcggcatg
     1321 tgcggcgtcg ccgcggctgt ggcgaatgcc gtctacaacg cgacgggcgt gcgggtgcgc
     1381 gactacccga tcacgctcga caagctgctg gaccggctgc cgccagtggc ctgaaacccg
     1441 cggggaagca ggcggcggcg gcgggtaagc ccggtgccgc gagtccgtct cgcttgatac
     1501 gattttttcc ggaacgcccg ctccaagggc gtcggagaca agatggactc gagcatttcc
     1561 agcagcgccg cccacggcgc gcatggcgcg gatgtggccc tgtaccgcgc catggtgcgg
     1621 atacgggcct tcgagaacgc ggcggagacc gccagccagg gcggcgtgag cgcgtacggc
     1681 cagcaggcgg cgggggccgc caaggtccgc gggccgctgc acctgtcgac agggcaggag
     1741 gcggtgccgg ccggcgtctg cgctcatctg cgccccgccg actacctcac ctccactcac
     1801 cgcggccatg gccacacgct ggccaagggc gccgacctcg cgcggatgat gtgcgagctg
     1861 ttcggcaagg ccaccggctt caacggcggc aagggcggct cgatgcacat cgcggatttc
     1921 tcggtcggca tgctgggcgc caacggcgtg gtggcggcgg ggctgccgat cgcggtgggc
     1981 gcggcgcacg cgcagaagct tctgaagaag agcgacgaca tcaccgtctg cttcttcggc
     2041 gacggcgcga tcaaccgcgg gcctttcctt gaagcgctga actgggcgcg cgtgtacgag
     2101 ctgccggtgc tcttcgtctg cgaggacaac cgctggagcg cgaccaccgc gagcggcccg
     2161 atgacggccg gcgaaggcgc ctccgcgcgc gccgagtcgc tcggcatcgc gtccacgcag
     2221 gtcgacggca acgacgtgtt cgcggtgcac gctgccgccg cgaagctggt ggccgaggtg
     2281 cgcaccggcg gcgggccgcg cctgctgcat gcgctgacct atcgcgtgaa ggggcatgtg
     2341 tcggtcgacg tggcggcgta ccgcgatccg gccgagctgg cagcggcact ggagaccgat
     2401 ccgatcgcac gggcgcgcgg ccatctgctt tcggccggcg tcgccaccgc gacgctcgac
     2461 gccatcgaga acgccgcgcg cgacgaggtc gacaccgccc tcgcggtcgc cgacgcagcc
     2521 ccgtggcccg agccggccgc cgccttcacc gacgtgcaga ccgtcgggga gggccagtgg
     2581 cgatgaagca cgaactgagc tattcggaag ccgccctgct cgccctgcag cgcgagatgg
     2641 aagccgaccc gcgcgtggtg gtgctcggcg aggatgtggg ccgcggcggc atcttcggcc
     2701 agtacaaggg gctgcagcaa agcttcggcc ccgagcgcgt gatcgacacg ccgatcagcg
     2761 aggccgcgat catgggcgcc ggcgtgggca tggcgctcgc agggctgcgg ccggtggtcg
     2821 agatgcgcgt ggtcgacttc gcgctgtgcg gcatggacga gctggtgaac caggccgcca
     2881 agaaccgctt catgttcggc ggccagggcc gcgtgccgct ggtagcgcgc atgcccggcg
     2941 gcatctggga cgcctcggcc gcgcagcatt cgcagtcgct cgaagcctgg ttcgcgcacc
     3001 tgccgggcgt ggtggtggtg agcccctcga cgccgcagga caaccacggg ctgctgcgct
     3061 ccgccctgca gtgcggcgac ccggtggtct acatcgagca caagacgctg tggggcgcgc
     3121 gcggcgaggt cgacgaaggc atcgccgtgc cgctgggcaa ggcggcgcgc gtgcgcgagg
     3181 gcgatgcgct cacgctggtg agctggagcc ggcagatgca ggcctgctcg gcggcctgcg
     3241 acgcgctggc ggccgaaggc atcgcggcgg acctgatcga cctgcgcacg ctgtggccgt
     3301 gggaccgcga gacggtgctg tcgtcgtgcg cgcgcaccgg ccggctgctg gtggtccacg
     3361 aggcggtgca ggccgcgggc ttcggcgcgg agatcgcggc gagtgcagcg gaggccaccg
     3421 gctgccgcgt ggcgcggctg ggcgcgccgc gcattccggt gggctacgcg ccggtgctgg
     3481 aggcgcagtc gcgcgtcggc acggaagcga tcgtggccgc ggcgaagaag ctggtgggct
     3541 gaaggcaggc cgcctcgccg gaagcgatgg acccgctctt cttcaagctg gtgcgggtcg
     3601 tgaatctcac ggcgcggcct ttccatgaac gcgtgggccg cgagcaccgg ctcacgctca
     3661 acgaatggcg cacgatggcc gtgctcgccg cgcagcccgg gctcaacgcc acgcaggtcg
     3721 ccgaccagac cggcctcgac aagatggcgg tgagccgcgc cctcgccggc ctgctgcgcc
     3781 acaagcgcgt gcagcgccac gacgacccga ccgaccggcg ccgcagccgg ctctatctca
     3841 ccgccgccgg caaggcgctg cacgaaacgg tgggcgcgct cggccgagaa cgcgaagtcg
     3901 cactgttcgg cggcatcggt gcggtcgaac tggcgcagct cgacaccacc ctcgacaagc
     3961 tcatcgaggc agtgcagcgc ggcacaccct gaccgctcca caagcaggcc acggcccatt
     4021 ccagaacacc
//
LOCUS       DATCRO010000636        17419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_388976, whole genome shotgun sequence.
ACCESSION   DATCRO010000636 DATCRO010000000
VERSION     DATCRO010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388976"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VIN75_17375"
     CDS             complement(<1..1916)
                     /locus_tag="VIN75_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8975991.1"
                     /translation="MTRRVRSSVRRPATAAHAPSGPSRPRPSWRPALLVAGLPIWLAH
                     GVQAQDSAPPPAPQQVVVTGSNVRRTDTETPSPVQVMTAADIKNSGYTSVGDVLHGIT
                     ANNMGSLSQSAPSAFAAGGSGVALRGLTVGATLVLIDGHRMASYPMPDDGERDFVDIS
                     SIPVDAIERIEVLKDGASAVYGSDAIAGVVNVILKKTFTGLTMSAETGLSGHGDGATQ
                     HASITTGWGSLDRDGQNAYVSIEGRHANSILLKNRPYLARTDWTPYGGTNLTQGTDSG
                     ADSGASSGAGSGTGYLVDANGNRTWFPGCTSTQAAADQCGYTNPYQTLSPSTTNIDVL
                     GRYTKQLDGGWQANFQGSMLRSEAHQVGLFNNAYATGVGSGGNGGGANLFHFGPGAAP
                     TPAFPDQFPFVFTVPATYPGNTTGDTANLVYDFLDIGPLEQRTVSTSWRAVGELAGSV
                     ANWDLNAAIGYTHVGTKASFYHYIDFPAMQDALDSGAYLVGGSNSAAELATVAPGASS
                     TSTNTLTFASLRGTHTLATLEGGDLGLGLGLDFTHRQLKELFPPSFVNGAQAIGIYSF
                     AEGKQTVSAAYVELVAPVTKALELDVAGRVDHYDTTGTSATPKAGFKFTPVRELTLRG
                     TFSKGFRAPNPAETG"
     gene            complement(1961..3079)
                     /locus_tag="VIN75_17380"
     CDS             complement(1961..3079)
                     /locus_tag="VIN75_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8975992.1"
                     /translation="MKSPLHVLVLLAAASALALAGAAARAAEEEKVLNVYNWSDYIAD
                     DTVRNFEKETGIEVHYDNFDSNEILHSKLVAGRSGYDIVVPSSSWARVQIQNNLYAPL
                     DKSQLPNLKYVDPAVAAQLARIDPGNAHVVDWLWGFTTVGINVGKVRAALGALPMPAN
                     AWDLLFKPEYVARLKSCGVSLLDSGDEVIPAALHYLGRPIDSKDPADYRKALELLRGI
                     RPSVTMFSSSSYINDMASGQVCVALGWSGDINIARRRAVDNKTGQDIQALMPATGGLL
                     FFDVMAIPADAPHKGNALKWINYILRPQVDAALTNKVFYANPNVASRQFIAPAIAGDH
                     TVFPSAAEIAGMVGVADALSPDVRRMRTRTFTSFKTQR"
     gene            complement(3865..4329)
                     /locus_tag="VIN75_17385"
     CDS             complement(3865..4329)
                     /locus_tag="VIN75_17385"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY8975993.1"
                     /translation="MKLSTEWSWRFGVMPQPWFSVFAGLVACVLLGVSAMSTLRTAQF
                     DAGALRATATVLPASSGAPMTQPLAEFTDANGQAWRVRFRVRSSPPAHHAGEQVDVLY
                     HRESPADARFDTFLERRFLALLSGLLSLPFLLMSWLTWKFRRRLFPQYRQGR"
     gene            4462..5280
                     /gene="cyoA"
                     /locus_tag="VIN75_17390"
     CDS             4462..5280
                     /gene="cyoA"
                     /locus_tag="VIN75_17390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEY8975994.1"
                     /translation="MPHVATWQQGVLDPQGPIAAAERTILLNATVIMLAVIVPVIVLT
                     LAFAWWFRAGNLRARRDPDWAYSGAIEVTVWSIPALVILFLGGIAWIGSHDLDPARPV
                     ASPTPPLTVQVVSLDWKWLFIYPDLGVASVNRLVVPAGRPLRLRLTSATVMNSFFVPQ
                     LGSQIYTMGGMATTLHLIADRPGTYAGLSANFSGDGFSDMRFDAVALAPDEFARWLAG
                     VRSGAVPLDAARYAALARPGVAAAPQAYAPVAPGLFETILADTSRAPRMQAMGE"
     gene            5285..7249
                     /gene="cyoB"
                     /locus_tag="VIN75_17395"
     CDS             5285..7249
                     /gene="cyoB"
                     /locus_tag="VIN75_17395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEY8975995.1"
                     /translation="MGNLTWDAIPLHEPIPLISGAVVVLTLLAVLALVTLKGWWPYLW
                     REWITSTDHKRIGVMYVLLAIVMLVRGFADALMMRSQLALAAGNARGYLPPSHYDQIF
                     SAHGTIMIFFVGMAFMVGLMNFVVPLQIGARDVAFPVMNSVSFWLTAAGALLINVSLV
                     VGEFARTGWLAYPPLSELAYSPGVGVDYWLWSLQIAGAGTLLSGINLTTTILKMRTPG
                     MGYGRVPIFTWTALASNLLIIAAFPILTGSLAMLALDRYLGFHFFTNEAGGNMMMYVN
                     LIWAWGHPEVYILVLPAFGIYSEVFPTFSGKRLFGYRSMAAATMGICVISFMVWLHHF
                     FTMGAGADVNAFFGIATSIIAIPTGVKIFNWLFTMYGGALRFELPLMWSIGFMLLFVI
                     GGATGVLLAIPPADFQLHNSQFLIAHFHNVIIPGVLFGAFAGYNFWFPKAFGFRLHRG
                     LGVASFWCWFIGFLLAFMPLYALGLAGATRRMQHYPETQWQPLMIVAFVGALVILAGI
                     VLMLAQLVVSIRRRERLRDATGDPWDGRTLEWATPSPPPPWNFAALPQVDGIDAWWEA
                     KRCNGGALKPAEGQGGHALHMPRPTPLGVVVAFFAFLAGFGAVWHIAWMAWGGLACIV
                     LACLVRAWRTDREVAVDPARIAAQEQGVRA"
     gene            7246..7866
                     /locus_tag="VIN75_17400"
     CDS             7246..7866
                     /locus_tag="VIN75_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8975996.1"
                     /translation="MSAVAAADVRDAGVPVSERGPSPRKVIISYGFWLFLLSDIIVFS
                     AFFAAYAVLAGRDAGGPRGTQLFDRGHVLVETGCLLFSSFACAMMSVAIELRRRALMV
                     LGAAFTFALGAAFLALELGEFGALLARGATPQRSAFLSAFYALVGLHGLHVATGLAWL
                     VVMMAQAFTLGWRPMVLCRLACFSLFWHALDIVWIGVFTIVYLGAH"
     gene            7866..8255
                     /locus_tag="VIN75_17405"
     CDS             7866..8255
                     /locus_tag="VIN75_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY8975997.1"
                     /translation="MDERDERHEQSAGHEQPGQSPDWKSGLVKPAMGLAASIVLTLAS
                     FWAAGTHLVWGPGVPVLLAALAIGQMGVHLVFFLHVSSGPESTNTVLALAFGVFVVAL
                     VVFGSLIIMANLDAGMGACAMHGSPCR"
     gene            8417..9841
                     /locus_tag="VIN75_17410"
     CDS             8417..9841
                     /locus_tag="VIN75_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8975998.1"
                     /translation="MHLQLDNKGPKHEQLARSLRQAIRSGQLQSGSKLPSTRMLSENL
                     GLSRNTVLRAYEQLRIERLGVMREGSGTYVNDVPLARVPVTAPRRVPAQSAFAARLRD
                     LPPITLGSGPAGLRYDLQYGAPLVNTKLVSAWSTALATAAATTSTGYPHPQGLPELRD
                     SISRFLATWRGVVADPRDIVVVSGTQQALSLSARILLDVGATAAMEDPYYELASHVLR
                     AQGAEVVGVRTDDEGLVVDELPRQARLVHVTPSHQFPSGLTMSLRRRRQLLRFAEETS
                     SWIFEDDYDGELSHGATPISALRSLDTGDRVIYVGSFSKMMFPSLRLAYVVCPAGLRK
                     DFVRAKMLEDLGCPAIEQAAMHLLFKRGAFDRHLGATVLELRRRRSALLAGLARHAAG
                     RIDVADSQLGMHVIGWLPGFDAARVDRLIAAARMQGLGLHPVHPYYRVPPPRPGLLLG
                     FAGLFPSQIDEATRILGDCLRAVS"
     gene            10087..10791
                     /locus_tag="VIN75_17415"
     CDS             10087..10791
                     /locus_tag="VIN75_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8975999.1"
                     /translation="MFAYTLSHEFPPARVLASREARASMPIRVLSVGDDPLIHEGIRA
                     LLGAEDDMALVGEVGSEDFDSIQFASLLPAVVLIGTELSGQGTVRAIARLRALVPSAR
                     VIVIASRSGDARARRAIDAGAQGCLLSSNVRGALAQAVRAVASGRRFIDGEAAIEWMQ
                     SRTGEELKAREVQVLELVAAGKANKEIAFELSTTEGTVKNYVKRILGKLHASDRTHAV
                     VIGIARGIIEISAISS"
     gene            complement(11023..13914)
                     /locus_tag="VIN75_17420"
     CDS             complement(11023..13914)
                     /locus_tag="VIN75_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976000.1"
                     /translation="MNTTTTFQRTTLCRCILGALGALASGTPTLALAQAPAAPDADVV
                     QRVEITGSSIKRVDAETALPVQTVTREQIEKLGVTNTEQLLQNISANSSAGGTTSMQN
                     AGQSTYGEATASLRGLGSQRTLVLVNGRRIANYATDGTSVDINSIPLSAIDRVEVLKD
                     GASGVYGSDAVAGVINFILRKDFHGVETTAYFGKPTTSGGGKSGKFGLVAGFGDMNTD
                     HVAAMFSLDVAHDASIQGYQRKYANHAWSDDGLFDISATATGAIRTFDPNILTSQNQG
                     KALYADGSGIGNILGPTSPDSSANNCTQNGMSFDVNLNTCRYNPSPLVPLTPDVTRVN
                     VAGTLRGRIDDDNEWFAEAFASNAKTTISEQASPYSASFLATDPLFHKIGVDPVIVMD
                     PTSPYYPTAYLNAVNTVNAAYNAAHPTSPQKPIVNVGVDPITVSYRALDGGGRLHTDN
                     ATLYHFVTGLTGTLKDWDYDATYSHNQSSVAESTQRGYQNQTQLVELLSHNDAFNPFA
                     AQQTPALAAQILATNYNGNIIRSTLKTDAIDLKVQHELAKLAGGPLALAAGISLRRES
                     IDLEPSAAYQSGDVAGYGAAVLPFAASRNAHSIYGEVDAPFLKSLEADLSVRNDHYPN
                     ASSTNPKLSLKFRPLDQIAFRGAVGTGFREPSLPELDTPLAPATTNIFTDPVTHVPGQ
                     FNEQVGGNPKLVPEKSKQFSFGTIVEPVRGLSASVDYYHVKIDHAIISLDAQTVVNLA
                     AAGNPVYVPLVSRDNSGNITNIIANNVNSGGVTTAGIDLDVKWQGLRTPAGQFGAELA
                     GTWIQKYDLTTIDGTVQHSVGRTTDPTDSAINAVTNGGLIFRWRHAMTVNWKYDAWGL
                     ALTQNFQSGYRDAPPAIDDCDPSDYTAPDAPPAVCRDGRRVSSFQTWDLQGEYAGVKN
                     MSLRLGLKNMFNRQPFPVTTNGIYFQTGYDPTWYDPHGRFVYASVTYKF"
     gene            14340..14870
                     /locus_tag="VIN75_17425"
     CDS             14340..14870
                     /locus_tag="VIN75_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8976001.1"
                     /translation="MNKIRILCAAVLAGASFAAAAQSGDDAAPTHWRFGVQLGTVADH
                     NNTEPVGQISLGYDFDRTWSVEALASFSLAFVRDGGLQQGDREFNDAVGARVLAALPM
                     GDRWRLVGGLGLVNFSEDVGVGMPAENDHVHKTSPMVSLAAMYRLGRRWSLGAEVSSF
                     TQPHSVNAGLRAEFHF"
     gene            complement(14920..>17419)
                     /locus_tag="VIN75_17430"
     CDS             complement(14920..>17419)
                     /locus_tag="VIN75_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8976002.1"
                     /translation="VDRIESQFAAMTAGGDDPTPALAPILDFIESDATLFDADRLAFR
                     DLLPSLQADVARNSTLGAIRLHRRIGDDIEALDEIQKSYDEEIRAIFSRFEQRAIVQK
                     RERWDDYVAHLARLYTREQILQAHGYVLPAVAAASAALPAPAPGSKPLPSGKPETGPE
                     IFGTGLPPKVVALTFDDGPHPVYTNEITAILKQYGAPGTFFEVGNNLGTVADGKARLG
                     RNAEISRQLLAEGYIVGNHSMTHAQLSKKTGDALKTEILDNDKLLKAIDPDRPPLFRF
                     PYGAHNAEGKEILAEQHLTSVMWNVDSMDWADPIPNSIADRVMREIDRNGRGIVLFHD
                     IHERTVKALPLVMSRLVAEGYQFAGWDGKSFTVQKPGDKRGFVDASQDAPGAAPTASA
                     AASMAAVSDTTTEGDSWAVVIGIDDYAHWPRLSHAARDAESVQGLLVDKLGFKSAHVI
                     ALQNASATRSNILAAFNEQLPKAGLKKTDNLFVFFAGHGATQHLTSGRDLGYIIPVEA
                     AADHVADDAIPMTELQNISEGLVARHVIFVMDACYSGLGLVRGGGRPGGGNFLRDNAH
                     RVGRQMLTAGGADQMVADGGPGGHSIFTWTLLQGLSGKADLNGDNIITGTELAAYIAP
                     AVASVSQQTPAFGSLPGSEGGEFVFTLAGAEEYISDATQQLPADAIALNTKLEKTTAV
                     VAASAATDGVVVKDLQGRDQKIAAPAAAPVSDRQQARQLNDRGMQFFKEKNYAAARDL
                     FAQSLKLQPDFALAANNLGFVYWKMNDDANAVKYFDAALKIDPSRAVAYVNLGDALQR
                     QGDKDGARKAFETYLQLAPAGGLAQHAKDAIAAL"
BASE COUNT         2327 a         5867 c         6276 g         2949 t
ORIGIN      
        1 ccggtctcgg cgggattggg cgcgcgaaag cccttggaga acgtgccgcg cagcgtcaac
       61 tcgcgcaccg gcgtgaactt gaagccggcc ttgggcgtgg ccgatgtccc ggtcgtgtcg
      121 tagtggtcga cgcggccggc gacgtccagc tcgagcgcct tggtcaccgg cgcgacgagc
      181 tccacgtacg ccgccgagac cgtctgcttg ccctcggcga acgagtagat gccgatggcc
      241 tgcgcgccgt tgacgaacga cggcgggaac agttccttga gctgccgatg cgtgaagtcc
      301 aggcccagtc ccagcccgag gtcgccgcct tccagcgtgg ccagcgtgtg ggtcccgcgc
      361 aggctggcga aggtgagcgt gttcgtcgag gtcgacgacg cgccgggtgc gacggtcgcg
      421 agctcggccg ccgagttgct gccgcccacc agataggcgc cgctgtcgag cgcatcctgc
      481 atcgccggga aatcgatgta gtggtagaag ctcgccttcg tgccgacgtg ggtgtagccg
      541 atcgcggcgt tcaggtccca gttggccacc gagccggcca gttccccgac cgcgcgccag
      601 gacgtggaga cggtccgctg ctcgagcggg ccgatgtcga ggaagtcgta gaccaggttc
      661 gcggtgtcgc cggtcgtgtt gcccgggtag gtcgccggca cggtgaacac gaacgggaac
      721 tggtcaggga acgccggcgt cggcgcggcg ccgggcccga agtggaacag gttcgctccg
      781 ccgccgttgc cgccgctgcc caccccggtg gcgtaggcgt tgttgaacag gccgacctgg
      841 tgcgcctcgc tgcgcagcat cgagccctgg aagttggcct gccagccgcc gtccagctgc
      901 ttcgtgtagc gccccagcac gtcgatgttc gtggtcgacg gcgacagcgt ctggtacggg
      961 ttcgtgtagc cgcactggtc cgccgcggcc tgggtggacg tgcagccggg gaaccaggtc
     1021 cggttgccgt tcgcgtcgac gaggtagccg gtgccgctgc cggcgccgct cgaggcgccg
     1081 gagtcggcgc ccgagtccgt gccctgcgtg aggttggtgc cgccataggg cgtccagtcg
     1141 gtgcgcgcga ggtagggccg gttcttcagc aggatcgagt tcgcgtgccg gccctcgatc
     1201 gacacgtacg cgttctggcc gtcgcggtcc aggctgcccc agcccgtggt gatgctggcg
     1261 tgctgcgtgg caccgtcgcc gtggccgctg aggccggtct cggcgctcat cgtcaggccc
     1321 gtgaaggtct tcttcaggat cacgttgacc acgcccgcga tggcgtccga gccgtagacc
     1381 gccgaggcgc cgtccttgag cacctcgatg cgctcgatcg catcgaccgg gatggacgag
     1441 atgtcgacga agtcgcgctc gccgtcgtcg ggcatcgggt acgacgccat ccggtgcccg
     1501 tcgatcagca cgagcgtcgc gccgacggtc agcccgcgca gcgccacgcc gctgccgccg
     1561 gccgcgaacg cgctgggcgc cgactgcgac agcgagccca tgttgttggc cgtgatgcca
     1621 tgcagcacgt cgccgacgct ggtgtagccg ctgttcttga tgtcggcggc cgtcatcacc
     1681 tgaacggggc tgggcgtttc ggtgtccgtg cggcgcacgt tcgagccggt cacgacgacc
     1741 tgctgcggcg ccggcggcgg ggcgctgtcc tgcgcctgga cgccgtgggc gagccagatc
     1801 ggcagtccgg cgacgagcag tgccggccgc caggacggcc gcggccggga cgggccggac
     1861 ggggcatgcg ctgcggtggc tggacgacgg acggaggatc ggactcgacg ggtcatgggc
     1921 atcttcctgg tggttgcgga tcttcttggg tggactggct tcagcgttgc gtcttgaacg
     1981 acgtgaacgt gcgggtgcgc atgcggcgga catcggggct gagcgcatcg gcgacgccca
     2041 ccatccccgc gatctcggcc gccgagggga agacggtgtg gtcgccggcg atggcgggcg
     2101 cgatgaactg cctggaggcc acgttggggt tcgcgtagaa caccttgttg gtgagcgccg
     2161 cgtcgacctg cgggcgcagg atgtagttga tccacttgag cgcgttgccc ttgtgcggcg
     2221 cgtccgccgg gatcgccatc acgtcgaaga acagcaggcc gcccgtggcg ggcatcagcg
     2281 cctggatgtc ctggccggtc ttgttgtcga cggcgcgccg gcgagcgatg ttgatgtcgc
     2341 cggaccaccc gagggccacg cagacctgcc cgctggccat gtcgttgatg tagctcgacg
     2401 agctgaacat cgtcaccgac gggcggatgc cgcgcagaag ttcgagcgcc ttgcggtagt
     2461 cggcgggatc cttgctgtcg atcgggcgcc cgaggtagtg cagcgccgcg gggatcactt
     2521 cgtcgcccga gtcgaggagc gagacgccgc aggacttgag cctggcgacg tattcgggct
     2581 tgaacagcag gtcccacgcg ttggcgggca tgggcagcgc gccgagcgcc gcgcgcacct
     2641 tgcccacgtt gatgccgacc gtggtgaagc cccacaacca gtccaccacg tgcgcgttgc
     2701 ctggatcgat gcgcgccagc tgcgccgcga ccgccgggtc gacgtacttc aggttcggca
     2761 actgcgactt gtccagcggc gcgtagagat tgttctggat ctgcacgcgc gcccagctcg
     2821 acgacggcac gacgatgtcg tagccggacc ggcccgccac cagcttcgaa tgcaggatct
     2881 cgttgctgtc gaagttgtcg tagtggacct cgatcccggt ttccttctcg aagttcctga
     2941 ccgtgtcgtc ggcgatgtag tcggaccagt tgtagacgtt gaggaccttc tcctcctcgg
     3001 cagcgcgcgc ggcggcgccg gccagcgcca gcgcggacgc ggcggcgagc aggaccagga
     3061 cgtgcaatgg cgacttcatc ggacaagctc ctttgcagtg ggcggcggcg gacgcgcgcg
     3121 cggccactcg gacgggaaac gggcggccgc acgcgcggcg agacgggccg gggccgtggc
     3181 gcggcgcagc ggggctgcgg gggggggcgc aggcatcgat cggatcgatc ggctccccgt
     3241 tcgagaacgc aagtcgcggc cgcggcagat cgtgccgtcg gcgacggcgc gcggcatcgc
     3301 cgcgtccgtc attccgccgt cgcgcggatc gcgttcgctg gccgggtagg cggtcgaatc
     3361 gatcgtcggg acttcatggc gagctcgcaa gttgcgggcc cgtggggtcg gaaaataccc
     3421 ctgaagcggc gtgcggtggc atggattcat gttgccgaca gcttgtgtcg gcgccaggca
     3481 gtcgcatcgc taaccggatg atgaatcgga cacgggagga tcgatgcaat ttgcgcgctc
     3541 cggtgtgcag ggttagcccg cgatcaagca acaaaattgc gcgaaccgtt ccctcgctgg
     3601 agcaggatgc gaaattgtca gcgattggcg tgatccctcc ggcgccgagt gcgccgaccg
     3661 gcgtgtgtcg ctgcgggacg tcgcttgcag cggccgaggg cgtcgcgcag gcggctcagg
     3721 cgatccgccg caccaccgcc cacacctcgc gcaccaccgg atgcccgccg atctcgctca
     3781 cgcgcaccag atcgccgcgc gggccgttgg caggagaacg tcgtgcgccg aagcggcacg
     3841 tcgccgcggc cggcggatgc ccggtcagcg gccttgccgg tactgcggga acaggcggcg
     3901 cctgaacttc cacgtcagcc acgacatcag caggaagggc aggctcagca ggccgctcag
     3961 cagcgcgagg aagcgccgct cgaggaaggt gtcgaagcgc gcgtccgccg ggctctcgcg
     4021 gtggtacagc acgtccacct gttcgccggc atggtgcgcc ggcggactgc tgcggacgcg
     4081 gaagcgcacg cgccaggcct gcccgttcgc gtcggtgaac tccgccaggg gctgcgtcat
     4141 cggggccccg ctgctggccg gcaggacggt ggccgtcgcc cgcaacgcgc ccgcgtcgaa
     4201 ttgcgcggtg cgcagcgtgg acatggcgga cacgcccagc agcacgcagg cgacgagccc
     4261 cgcgaacacg gagaaccagg gctgcggcat gacgccgaat cgccaggacc attcggtgga
     4321 cagcttcatc gggccgtcct tcgcaagccg gcggcaagcc tgggcatcgt cgcgggagtc
     4381 atgggcccga ttgttgccca tgggagcgct tgcgaggccg tcgctcgacg gcacgcctcg
     4441 tcagccaccg aggagccact cttgccgcat gtcgccacct ggcagcaggg cgtgctcgac
     4501 ccgcaggggc cgatcgccgc cgcggagcgc acgatcctgc tgaacgccac ggtcatcatg
     4561 ctggccgtga tcgtgccggt gatcgtgctg acgctcgcgt tcgcctggtg gttccgcgcc
     4621 ggcaacctgc gcgcgaggcg cgacccggac tgggcgtatt ccggcgcgat cgaagtcacc
     4681 gtgtggtcca tccccgcgct ggtgatcctg ttcctcggcg gcatcgcatg gatcggctcg
     4741 cacgacctcg acccggcgcg gccggtggcc tcgcccacgc caccgttgac ggtgcaggtg
     4801 gtgtcgctgg actggaagtg gctgttcatc tatccagacc tgggcgtggc cagcgtcaac
     4861 cgcttggtcg ttcctgcagg aagacctttg cggcttcgcc tgacgtccgc caccgtgatg
     4921 aacagcttct tcgtcccgca gctgggctcg cagatctaca cgatgggcgg catggcgacg
     4981 acgctgcacc tgatcgccga ccggcccggg acctacgccg gcctgtcggc caacttcagc
     5041 ggcgacggct tctccgacat gcgcttcgat gccgtcgcgc tcgcgccgga cgagttcgcg
     5101 cgctggctgg ccggcgtgcg cagcggcgcg gtgccgctgg acgccgcgcg ctacgccgcg
     5161 ctcgcgcgcc ccggcgtggc cgccgcgccg caggcctacg caccggtggc gccgggcctg
     5221 ttcgagacga tcctggccga cacgtccagg gcgccgcgca tgcaggcgat gggagagtga
     5281 ccccgtgggc aacctgacct gggatgcgat cccgctgcat gaaccgatcc cgctgatctc
     5341 gggcgccgtc gtcgtgctga cgctgctggc cgtgctggcc ctggtcacgc tcaagggctg
     5401 gtggccctat ctgtggcgcg agtggatcac cagcaccgac cacaagcgca tcggcgtgat
     5461 gtacgtgctg ctggcgatcg tgatgctggt gcgcggcttc gccgacgcgc tgatgatgcg
     5521 ttctcagctg gcgctggccg cggggaacgc ccgggggtac ctgccgccgt cgcactacga
     5581 ccagatcttc tcggcgcacg gcaccatcat gatcttcttc gtcggcatgg ccttcatggt
     5641 cggcctgatg aacttcgtcg tgccgctgca gatcggcgcc cgcgacgtcg cgttcccggt
     5701 catgaactcg gtgagcttct ggctgacggc cgccggcgcg ctgctgatca acgtgtcgct
     5761 ggtggtgggc gagttcgcgc gcaccggctg gctggcctat ccgccgctgt ccgaactcgc
     5821 gtactcgccc ggcgtgggcg tcgactactg gctgtggtcg ctgcagatcg ccggcgccgg
     5881 cacgctgttg tcgggcatca acctgaccac caccatcctg aagatgcgca cgccgggcat
     5941 gggctacggc cgcgtgccca tcttcacgtg gaccgcgctg gcgtcgaacc tgctgatcat
     6001 cgcggccttc cccatcctca cgggctcgct ggccatgctg gcgctggacc gctacctcgg
     6061 cttccacttc ttcaccaacg aggcgggcgg caacatgatg atgtacgtca acctcatctg
     6121 ggcctggggc catccggagg tctacatcct ggtgctgccg gccttcggca tctactcgga
     6181 ggtgttcccc accttctcgg gcaagcgcct gttcggctac cgctcgatgg cggccgcgac
     6241 gatggggatc tgcgtcatct ccttcatggt ctggctgcac cacttcttca cgatgggcgc
     6301 gggcgcggac gtcaacgcgt tcttcggcat cgcgacgtcg atcatcgcga tccccaccgg
     6361 cgtgaagatc ttcaactggc tgttcacgat gtacggcgga gcgctgcgct tcgagctgcc
     6421 gctgatgtgg tccatcggct tcatgctgct gttcgtcatc ggcggcgcca ccggcgtgct
     6481 gctggccatc ccgccggccg acttccagct gcacaacagc cagttcctca tcgcgcactt
     6541 ccacaacgtg atcatccccg gcgtgctgtt cggcgcgttc gcgggctaca acttctggtt
     6601 ccccaaggcg ttcggtttcc gcctgcaccg cggcctgggc gtggcctcgt tctggtgctg
     6661 gttcatcggc ttcctgctcg cgttcatgcc gctgtacgcg ctgggcctgg ccggggccac
     6721 gcggcgcatg cagcactatc ccgagacgca gtggcagccg ctgatgatcg tcgccttcgt
     6781 cggcgcgctc gtgatcctcg cgggcatcgt gctgatgctg gcgcagctgg tcgtgagcat
     6841 ccgccggcgc gagcggctgc gcgacgccac cggcgaccca tgggacggcc gcacgctcga
     6901 gtgggcgacg ccgtcgccgc cgcccccatg gaacttcgcg gcgctgccgc aggtcgacgg
     6961 catcgatgcg tggtgggagg ccaagcgctg caacggcggc gcgctgaagc ccgccgaagg
     7021 gcagggcggc cacgcgctgc acatgccgcg gcccacgccg ctgggcgtcg tcgtcgcgtt
     7081 cttcgcgttc ctcgcgggct tcggcgcggt atggcacatc gcgtggatgg cctggggcgg
     7141 cctggcttgc atcgtgctcg cctgcctcgt gcgcgcgtgg cgcaccgacc gcgaggtggc
     7201 ggtcgatcca gcccggatcg ccgcgcagga acagggagtg cgcgcgtgag cgccgtcgcc
     7261 gccgcggacg tgcgcgacgc cggcgtgccg gtgtccgagc gcggcccgtc gccgcgcaag
     7321 gtgatcatca gctacggctt ctggctgttc ctgctcagcg acatcatcgt gttctcggcc
     7381 ttcttcgcgg cctatgccgt gctcgcgggg cgcgatgccg gcggcccgcg cggcacgcag
     7441 ctgttcgacc gcggccacgt gctggtcgag accggctgcc tgctgttttc gagcttcgcc
     7501 tgcgcgatga tgtccgtggc catcgagctg cgccgccgcg cgctgatggt gctgggtgcc
     7561 gcgttcacgt tcgcgctcgg cgcggctttc ctcgcgctgg agctggggga gttcggcgcc
     7621 ttgctggcgc gcggcgcgac gccgcagcgc agcgcgttcc tgtccgcctt ctacgcgctg
     7681 gtgggcctgc acgggctgca cgtggccacc ggcctggcgt ggctcgtcgt gatgatggcc
     7741 caggccttca cgctgggctg gcgcccgatg gtgctgtgcc gcctggcctg cttcagcctg
     7801 ttctggcacg cgctggacat cgtctggatc ggcgtgttca ccatcgtcta cctgggagcg
     7861 cactgatgga cgaacgcgac gaacgccatg agcagtcggc cggccacgag cagccgggcc
     7921 agtcgcccga ctggaaaagc ggcctggtga agccggcgat gggcctggcc gcgtccatcg
     7981 tgctgaccct ggcatcgttc tgggcggccg gcacgcacct ggtgtggggc cccggcgtgc
     8041 cggtgctgct ggccgcgctg gcgatcggcc agatgggcgt gcacctggtc ttcttcctgc
     8101 atgtgtccag cggcccggag agcaccaaca ccgtcctggc gctggccttc ggcgtgttcg
     8161 tggtggcgct ggtggtgttc ggctccctga tcatcatggc caacctcgac gcggggatgg
     8221 gcgcctgcgc aatgcacggt tcgccgtgca ggtaggcgcg cgcggcgcct gcatgcccgc
     8281 cgcgggaagc cgcaactggg cgcgttccag ccgccgacag gcactggatt cccgctgttc
     8341 gtcgcatcgg cggcgcgtac gcggggcggt attgctagat tggtcgtcaa ccagtgccgg
     8401 cagggcgggc gacaagatgc atctgcaact agacaacaag ggccccaagc acgagcagct
     8461 cgcgcgctcg ctgcggcagg ccattcgcag cggccagctc cagtcgggca gcaagctgcc
     8521 gtccacgcgc atgctgtccg agaacctcgg gctctcgcgc aacaccgtgc tgcgcgcgta
     8581 cgagcagttg cgcatcgaac ggctgggggt catgcgcgag ggctcgggca cctacgtcaa
     8641 cgacgtgccc ctggcgcgcg tgccggtcac ggcgccccgg cgcgtcccgg cgcagtcggc
     8701 gttcgccgcg cgcttgcgcg acctgccgcc catcacgctg ggcagcggcc cggccgggct
     8761 gcgctacgac ctgcagtacg gcgcgccgct ggtcaacacc aagctggtca gcgcgtggag
     8821 cacggcgctg gcgaccgcgg ctgcgaccac cagcaccggg tatccgcacc cgcaaggcct
     8881 gccggagctg cgcgattcca tctcccgctt cctggccacc tggcgcggcg tcgtcgccga
     8941 tccacgcgac atcgtcgtcg tcagcgggac gcagcaggcg ctttcgctgt cggcgcgcat
     9001 cctgctcgac gtgggcgcga cggccgccat ggaggatccg tactacgagc tcgcctcgca
     9061 cgtcctgcgc gcccagggcg ccgaggtggt gggcgtgcgc accgacgacg agggcctggt
     9121 cgtcgacgag ctgccgcgcc aggcccggct ggtgcacgtc acgccgtcgc accagttccc
     9181 gtcgggcctg acgatgtcgc tgcgccgccg ccggcagctg ctgcgcttcg ccgaggagac
     9241 ctcgagctgg atcttcgagg atgactacga cggcgagctg agccatggcg cgacgccgat
     9301 ctcggcgttg cgctcgctgg acaccggcga ccgcgtgatc tacgtgggca gcttctcgaa
     9361 gatgatgttc ccgtcgctgc gcctggccta cgtcgtctgt ccggcgggcc tgcgcaagga
     9421 cttcgtccgc gcgaagatgc tggaggacct cggctgcccg gccatcgagc aggcggcgat
     9481 gcacctgctg ttcaagcgcg gcgcgttcga ccggcatctg ggcgccacgg tgctcgagct
     9541 caggcgacgg cgctccgcgc tgctggccgg actggcgcgc cacgccgcgg gacgcatcga
     9601 cgtcgccgat tcgcagctgg gcatgcacgt catcggctgg ctgccggggt tcgacgcggc
     9661 gcgggtggac cgtctcatcg cggccgcgcg catgcagggc ctgggcctgc atcccgtcca
     9721 cccgtactac cgcgtgccgc cgccgcgccc gggcctgctg ctcggcttcg ccggcctgtt
     9781 cccgagccag atcgacgagg cgaccaggat cctcggcgac tgcctgcggg cggtgtcgtg
     9841 aggtgggccc gatgccgcga ccttcgatga aagcttcagg acagcaagtg ataccttgga
     9901 tttggacaaa tgcggttcct ttcgcgtccg gatcgcgctc taaagtgacc tccatgtcgc
     9961 catgcggcac ggcgggctcg aagggacggc agggacatcg tcctcgcgcc gccggatgct
    10021 cgacggccgt cgtcctggtg cgattcgagt ccgcttgccg cggcccggtg cctggagcaa
    10081 cccacgatgt tcgcctacac cctttcgcat gagtttcctc ccgcgcgcgt gctcgcctcc
    10141 cgcgaggcgc gcgcgtcgat gccgatccgc gtgctctcgg tcggcgacga tccgttgatc
    10201 catgaaggga tccgcgcgct gctgggcgcc gaggacgaca tggcgctggt gggcgaggtc
    10261 ggcagcgagg acttcgattc catccagttc gcgagcctgc tgccggcggt ggtcctgatc
    10321 ggcaccgagc tgtcgggcca gggcaccgtc cgcgcgatcg cccggctgcg cgcgctcgtc
    10381 ccgtcggccc gcgtgatcgt catcgcctcg cgcagcggcg acgcgcgcgc gcggcgggcg
    10441 atcgatgccg gcgcgcaggg ctgcctgctg tcgtcgaacg tgcggggcgc gctggcccag
    10501 gcggtccgcg cggtcgcctc cggccgccgc ttcatcgacg gcgaggccgc catcgagtgg
    10561 atgcagagcc ggaccgggga agagctcaag gcgcgcgagg tgcaggtgct cgagctggtg
    10621 gccgcgggca aggcgaacaa ggagatcgcg ttcgagctgt cgacgaccga aggcaccgtc
    10681 aagaactacg tcaagcgcat cctcggcaag ctgcacgcgt ccgaccggac gcacgccgtc
    10741 gtcatcggca tcgcgcgggg catcatcgag atcagcgcga tctccagctg agcggcgtcg
    10801 ctgcacgtcc ctgacgccgg ctccccgcca ccctcatggc agggcggatc ccgcgactgc
    10861 gcgcaggatg gcggcacggt cggcgacagc tttcccgtcg aggcttcgag ccgtcccggc
    10921 ttcccccccg cgcttcggcc gagaaccaaa aaaaaaagag aggcggactc aggattccgc
    10981 ctctgccaag atcaagagct gcctctcgat caacctttgg ggtcagaact tgtaggtcac
    11041 cgacgcgtag acgaagcggc cgtgcgggtc gtaccaggtc gggtcgtagc ccgtctggaa
    11101 gtagatgccg ttggtcgtga cggggaacgg ctggcggttg aacatgttct tcaggccgag
    11161 gcgcaaggac atgttcttca cgccggcata ttcgccctgc aggtcccagg tctggaacga
    11221 cgagacgcgg cggccgtcgc ggcagaccgc gggcggagca tccggcgccg tgtagtcgga
    11281 cggatcgcag tcgtcgatcg cgggcggcgc gtcgcggtag cccgactgga agttctgcgt
    11341 cagcgccagg ccccaggcgt cgtacttcca gttcaccgtc atcgcgtggc gccaacggaa
    11401 gatcaggccg ccgttggtca ccgcgttgat cgccgagtcc gtcgggtcgg tcgtgcggcc
    11461 gacgctgtgc tgcacggtgc cgtcgatcgt cgtcaggtcg tacttctgga tccacgtgcc
    11521 ggccagttcg gcgccgaact gtcccgccgg cgtgcgcagg ccctgccact tgacgtccag
    11581 gtcgatgccc gcggtggtga cgccgccgct gttgacgttg ttggcgatga tgttggtgat
    11641 gttgccggag ttgtcgcgcg agaccagcgg cacgtacacc gggttgcccg cggccgccag
    11701 gttcaccacc gtctgggcgt cgagcgagat gatcgcgtgg tcgatcttca cgtggtagta
    11761 gtcgacgctg gccgacaggc cgcggaccgg ctcgacgatc gtgccgaacg agaactgctt
    11821 ggacttctcg ggcaccagct tcgggttgcc gccgacctgc tcgttgaact ggcccggcac
    11881 atgggtgacg gggtcggtga agatgttcgt cgtggcgggc gcgagcggcg tgtccagctc
    11941 gggcagcgat ggctcgcgga agcccgtgcc gacggcgccg cgaaacgcga tctggtcgag
    12001 cggccggaat ttcaggctca gcttcgggtt ggtggaggac gcgttggggt agtggtcgtt
    12061 gcgcaccgac aggtcggcct cgagcgactt caggaacggg gcgtcgacct cgccgtagat
    12121 cgagtgcgcg ttgcgcgacg cggcgaacgg cagcaccgcg gcgccgtagc cggcgacgtc
    12181 gccgctctgg taggccgcgc tcggctccag gtcgatcgat tccctgcgca gcgatatccc
    12241 ggcggcgagc gccagcgggc cgcccgccag cttggccagc tcgtgctgca ccttcaggtc
    12301 gatcgcatcg gtcttgagcg tcgagcggat gatgttgccg ttgtagttcg tcgccaggat
    12361 ctgggcggcc agcgccggcg tctgctgcgc ggcgaacgga ttgaaggcat cgttgtggct
    12421 gagcagctcg accagctgcg tctggttctg gtagccgcgc tgcgtcgatt ccgcgaccga
    12481 gctctggttg tggctgtagg tcgcgtcgta gtcccagtcc ttgagcgtcc cggtcaggcc
    12541 ggtcacgaag tggtacagcg tcgcgttgtc cgtgtgcagg cggccgccgc cgtccagcgc
    12601 gcgatagctg accgtgatcg ggtccacgcc gacattgacg atgggcttct gcggactcgt
    12661 cgggtgcgcc gcgttgtagg ccgcgttcac cgtgttcacc gcgttgaggt aggccgtcgg
    12721 atagtacggg ctcgtcggat ccatgacgat cacgggatcc acgccgatct tgtggaacag
    12781 cggatcggtg gccaggaacg aggcgctgta cggcgaggcc tgctcgctga tggtggtctt
    12841 ggcgttcgac gcgaacgctt ccgcgaacca ctcgttgtcg tcgtcgatgc ggccacgcag
    12901 ggtgcccgcg acgttcacgc gcgtgacatc cggcgtcagc ggcaccagcg gcgaagggtt
    12961 gtagcggcag gtgttgaggt tcacgtcgaa cgacatgccg ttctgcgtgc agttgttggc
    13021 cgagctgtcg gggctggtcg ggcccaggat gttgccgatg ccgctgccgt ccgcgtacag
    13081 ggccttgccc tggttctgcg aggtcaggat gttggggtcg aacgtgcgga tcgcgcccgt
    13141 ggcggtcgcg ctgatgtcga acaggccgtc gtcgctccat gcgtggttgg cgtacttgcg
    13201 ctggtagccc tggatcgacg cgtcgtgcgc gacgtccagc gagaacatgg ccgcgacgtg
    13261 gtcggtgttc atgtcgccga agccggcgac gagtccgaac ttgccgctct tgccgccgcc
    13321 ggaggtggtc ggcttgccga agtaggcggt cgtctcgacg ccgtggaaat ccttgcgcag
    13381 gatgaagttg atgacgccgg cgacggcgtc cgagccgtac acgccggagg cgccgtcctt
    13441 gagcacttcc acgcggtcga tggccgacag cgggatcgaa ttgatgtcga ccgacgtgcc
    13501 gtccgtcgca tagttcgcga tgcggcggcc gttgaccagc accagggtgc gctgcgagcc
    13561 gaggccgcgc aggctcgcgg tggcctcgcc gtaggtcgac tgcccggcgt tctgcatcga
    13621 ggtcgtgccg ccggccgacg agttcgcgga gatgttctgc agcagttgct ccgtgttcgt
    13681 gacgccgagc ttctcgatct gctcgcgcgt caccgtctgc accggcagcg cggtctcggc
    13741 gtcgacccgc ttgatcgagg agcccgtgat ctccacgcgc tgcacgacat ccgcgtccgg
    13801 cgcggcgggc gcctgcgcca gcgcgagggt cggggtgccc gaggcaaggg cgcccagcgc
    13861 gccgaggatg caacggcaga gcgtggttct ctggaaggtg gtggtcgtgt tcattggatg
    13921 tggtgtgtga gtgaaccggc cccgggggct cgtgaccctc gtccgccggc gaatcgctgc
    13981 atcgaagtcg ccgtcgggcc cgcggtggcg gacgtctccg gcatgcctgc cggggtatcc
    14041 gtccatccgc gcccgccgcg acgcatctgg cctcgcgtcc cgccaggaaa cgagtcgcgg
    14101 cgtcgccgac gccctcggcg cagtgcacaa aatctatcga gccggcgcga gcgcgaaaag
    14161 tctcggagga gaggggcggc gagtctcttt aggcatcagc gtccccgctt cgcggcgcgc
    14221 ggcggcgccg cgcggcgagg gccggcccgc catccggggg gaagggaatg ccccgactca
    14281 tccatggccg gagcccgtgc gaactgcgga gagaatccct tcccgacaca gggagtcgaa
    14341 tgaacaagat ccgcatcctc tgcgccgccg tcctggccgg cgcctcgttc gccgccgccg
    14401 cccagtccgg cgacgacgcc gccccgacgc actggcgctt cggcgtccag ctgggcacgg
    14461 tggcggatca caacaacacc gaaccggtcg gccagatctc gctgggctac gacttcgatc
    14521 gcacctggtc ggtcgaggcg ctggccagct tcagcctcgc cttcgtgcgc gacggcggcc
    14581 tgcagcaggg cgaccgcgag ttcaacgacg cggtcggcgc gcgcgtgctc gcggcgctgc
    14641 cgatgggcga ccgctggcgc ctggtcggcg gcctgggcct ggtgaacttc tccgaggacg
    14701 tcggcgtcgg gatgccggcc gagaacgacc acgtgcacaa gacgagcccg atggtgagcc
    14761 tggccgccat gtatcgcctc gggcgccgct ggtcgctggg cgccgaggtg tcctcgttca
    14821 cgcagccgca cagcgtcaac gccgggttgc gcgccgaatt ccacttctga cgcggtggcc
    14881 gtcgcggccg ggcgttcacg ccgcgccgcg ggcggggcct cagagcgccg cgatggcgtc
    14941 cttggcgtgc tgcgcgaggc ctccggcggg cgccagctgc aggtaggtct cgaacgcctt
    15001 cctcgcgccg tccttgtcgc cctggcgctg cagggcgtcg ccgagattga cgtaggccac
    15061 ggcccgggac gggtcgatct tcagcgcggc atcgaagtac ttcaccgcgt tggcgtcgtc
    15121 gttcatcttc cagtagacga agcccaggtt gttggccgcc agcgcgaagt cgggctgcag
    15181 cttcagcgac tgcgcgaaca ggtcgcgcgc cgcggcatag ttcttctcct tgaagaactg
    15241 catgccgcgg tcgttgagct ggcgagcctg ctggcggtcg ctgacgggcg cggcggcggg
    15301 cgccgcgatc ttctggtccc tgccctgcag gtccttgacc acgacgccgt cggtggccgc
    15361 gctggcggcg acgaccgcgg tcgtcttctc cagcttcgtg ttcagcgcga tggcgtcggc
    15421 gggcagctgc tgggtggcat cgctgatgta ctcctccgcg cccgccagcg tgaagacgaa
    15481 ctcgccgccc tccgagccgg gcaggctgcc gaacgccggg gtctgctgcg acacggacgc
    15541 caccgccggc gcgatgtagg ccgccagttc ggtgccggtg atgatgttgt cgccgttgag
    15601 gtcggccttg cccgagagtc cctgcagcag cgtccacgtg aagatcgagt gcccgccggg
    15661 gccgccgtcg gcgaccatct ggtcggcgcc gccggcagtg agcatctggc ggccgacgcg
    15721 gtgggcgttg tcgcgcagga agttgccgcc gcccgggcgg ccgccgccgc gcaccaggcc
    15781 caggccgctg tagcacgcgt ccatcacgaa gatcacatgg cgcgcgacga ggccttccga
    15841 gatgttctgc agctccgtca tggggatcgc gtcgtcggcg acgtggtcgg ccgcggcctc
    15901 gaccgggatg atgtagccca ggtcgcggcc cgaggtgagg tgctgcgtgg cgccatggcc
    15961 ggcgaagaag acgaagaggt tgtccgtctt cttcaggccc gccttgggca actgctcgtt
    16021 gaacgcggca aggatgttcg agcgagttgc cgaagcgttc tgcagcgcga tgacgtgcgc
    16081 cgacttgaag ccgagcttgt ccaccagcag gccctgcacg gactcggcgt cgcgcgcggc
    16141 atggctcagc ctgggccagt gcgcgtagtc gtcgatgccg atcaccacgg cccacgagtc
    16201 gccctcggtg gtggtgtcgg acaccgcggc catcgacgcc gccgccgagg ccgtcggcgc
    16261 ggcgccgggc gcgtcctgcg acgcgtcgac gaagccgcgc ttgtcacccg gcttctgcac
    16321 cgtgaacgac ttgccgtccc agcccgcgaa ctggtagccc tcggccacca gccggctcat
    16381 caccagcggc agtgccttga cggtgcgctc gtggatgtcg tggaacagca cgatgccgcg
    16441 cccgttccga tcgatctcgc gcatcacgcg gtcggcgatc gagttgggga tcgggtcggc
    16501 ccagtccatc gagtcgacgt tccacatcac cgaggtcagg tgctgctcgg cgaggatctc
    16561 cttgccctcg gcgttgtggg cgccgtacgg gaagcggaac agcggcgggc ggtcggggtc
    16621 gatcgccttg agcagcttgt cgttgtccag gatctcggtc ttgagcgcgt cgcccgtctt
    16681 cttggacagc tgcgcgtgcg tcatgctgtg gttgccgacg atgtagccct cggccagcag
    16741 ctggcggctg atctcggcgt tcctgcccag cctggccttg ccgtccgcga cggtgcccag
    16801 gttgttgccg acctcgaaga acgtgcccgg cgccccgtac tgcttgagga tggcggtgat
    16861 ctcgttggtg tagaccgggt gcgggccgtc gtcgaaggtc agcgcgacga ccttgggcgg
    16921 caggccggtg ccgaagatct cggggccggt ctcgggcttg cccgagggca gcggcttcga
    16981 tccaggcgcc ggcgccggca gcgccgcgct ggccgcggcg accgcgggca gcacgtagcc
    17041 atgcgcctgc aggatctgct cgcgcgtgta gagcctggcg aggtgcgcga cgtagtcgtc
    17101 ccagcgctcg cgcttctgca cgatggcgcg ctgctcgaag cgcgagaaga tcgctcggat
    17161 ctcctcgtcg tagctcttct ggatctcgtc gagcgcctcg atgtcgtcgc cgatgcgccg
    17221 gtgcagccgg atcgcgccga gggtcgagtt gcgcgcgacg tcggcctgca ggctgggcag
    17281 caggtcgcgg aaggcgaggc ggtcggcgtc gaacagcgtg gcgtcgctct cgatgaaatc
    17341 caggatcggc gcgagcgccg gcgtggggtc gtcgccgccg gccgtcatcg cggcgaactg
    17401 cgactcgatc ctgtcgacg
//
LOCUS       DATCRO010000637         7785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_569672, whole genome shotgun sequence.
ACCESSION   DATCRO010000637 DATCRO010000000
VERSION     DATCRO010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569672"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VIN75_17435"
     CDS             complement(<1..951)
                     /locus_tag="VIN75_17435"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8976003.1"
                     /translation="MRFPKPGLLSLLIGAAPAMAPINAGTLPPTPSPPFKLTKVAQFD
                     QPWRIAFLPDGRMLVTEKPGKLFIATETGRKLEVAGVPAVHYQGQNGLLGVYLAPSYA
                     SDGAIYLTYSEPGPETGTSSLALARATLKMDADAASLEDLKVVWHDPVKGKGGQVGAA
                     VAFSPDRKFLFLTAGDRQRFTPAQDPNQPAGKILRLTLDGKPAPGNPMEGKVGATSVE
                     LIDPPRDTEAAKTAPVVRTVVWDGPNLTPAETWTTGHRTPYGLAFAPDGRLWELEHGP
                     RGGDELNLIEPGKNYGWPLVAYGTDYDGTPIPSPDTRPDLT"
     gene            1184..1744
                     /locus_tag="VIN75_17440"
     CDS             1184..1744
                     /locus_tag="VIN75_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976004.1"
                     /translation="MEEFDAFLRRVRDGVERGALTQQEAAHYVGEHVARQLYCSSATL
                     WTLAGPPGLRVLTRVGGFDATLRMPLAEPLQFTLSGPSAWHDALSNEGVFATTDTHAE
                     PRLAGDHAALAGLRRVRGLLQAAIGRGAAPWGLVSCAQYDAVRIWTRPEIVHLQHIAT
                     ALSARRPRDRAVAGAREAPPVSRAST"
     gene            1788..2543
                     /locus_tag="VIN75_17445"
     CDS             1788..2543
                     /locus_tag="VIN75_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8976005.1"
                     /translation="MHIPSLKEVVSAEEWQLRCDLAACYRLVAAYGWSDLVFTHISAR
                     VPGGDHSFLINPYGLMFDEITASSLVLIDQEGNKLRESPFPVNRAGFVIHSAIHAARE
                     DAQCVLHTHTRAGVAVSAQAGGVLPISQQSTFVLASLAYHDYEGVALRDEEKPRLQAD
                     LGTANYLMLRNHGLLTVGRSIAEAFLAMYMFESTCSIQLAAQAGGPLTTVQPEVLAGV
                     AESMRVQTGSQGGAFVWPALLRKVERMDPGYRT"
     gene            2694..3755
                     /locus_tag="VIN75_17450"
     CDS             2694..3755
                     /locus_tag="VIN75_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY8976006.1"
                     /translation="MKCHACGQDNADSALFCTACRRPLVAPTKAPTRLEPLAAAAAPS
                     VAALPPSPPPGDLGGRNRYAPPENGAAGKGSRMGDASEVLTDEEARAAVIGDANTVYY
                     LGRFDRIAHGEGGGWNWPAFLITWYWMLYRKMWVPALLYFFAPYVVSMVLVGVAAAAP
                     ALGGVLALVWLAAWLIGPGLMANGWYYRHVRNKIRDVRARGGSKDQIIARLEAAGGTS
                     NIAVIIVAVLAIPAFIGILAAVSLPAYQTYTVKAKVADAIIESTAVATVAGRQFEATG
                     QYPTDIDRIAQQVPQQSKYVRGFEIDGMSGTITVHIQATPAVDGSIQLTPYVDGNRHV
                     TWSCSTTDLLKYVPKSCRG"
     gene            complement(3771..4343)
                     /locus_tag="VIN75_17455"
     CDS             complement(3771..4343)
                     /locus_tag="VIN75_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976007.1"
                     /translation="MALHWVMPVFEIKPVFIDGTDAAAEWVDDRHSAWNEPLLRTTAP
                     LAERWMPPALELYRSEMSPTAVLFHPTAHVVSQGVRDELSHFQELEFLPVMVAGYGVF
                     FAMHVLASCELPAGSIADHGPGTDIALIRAFPKAFEPPAPFFRIRHPGTLTARGMAVC
                     VPDVYASEAGATAVMRVAGKFLRAVRVPMA"
     gene            4499..5110
                     /locus_tag="VIN75_17460"
     CDS             4499..5110
                     /locus_tag="VIN75_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976008.1"
                     /translation="MITKALLFRFESQTDEDTMDTFLEDLATDVSWEKATQAWFGIRY
                     MRGEFGMYSVYDDEAGREAHLQGDPARKVFAAEHKLMTAAPRVTKIELLGCKMPNALA
                     GVTKGLVLRFKAKPGKEAEVAKMMRDCVPLVEKEAGTLAWFANQYDESHFGTFAAFAD
                     TGARFAHLTGQIPRELGITGLSLLGGTPEVHMVDVMAGTARQR"
     gene            complement(5159..5467)
                     /locus_tag="VIN75_17465"
     CDS             complement(5159..5467)
                     /locus_tag="VIN75_17465"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY8976009.1"
                     /translation="MTAKLDSAQLPGLLSQLPGWSLDSKASSISRHLEFADFNEAFAF
                     MARVALAAERRDHHPDWSNSYNVVDVTLTSHDAKGLTMKDIELARFIDRTASGSGASA
                     "
     gene            complement(5583..6542)
                     /locus_tag="VIN75_17470"
     CDS             complement(5583..6542)
                     /locus_tag="VIN75_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976010.1"
                     /translation="MPAAPLLTHRHVEVFRAVMTAGSVTGAAAALFTSQPTISRELSR
                     MEQLLRLALFERVRGRLQPTAQAYALFDEVQRSYAGLERVADTALRLRQFTEGQLSLA
                     CLPAFSHALLPGACARFQAAHPGVSVSVNPYESPLLEERLAAQAHDLGVTEHDSALAG
                     AQPSLLLELDEVAVLPAGHPLLARRRLAAADFAGQAFVSFHPRDRYRQWVDKVFAEAG
                     VDRQLAVETDSAVAVCAMVRHGLGLAIVNPLTALALSGPESGIAVRPLAFAIPFRVYS
                     VRPAHRPGNPLVARFETALQAECDALREAIARAWERPARGRRG"
     gene            6624..>7785
                     /gene="lysA"
                     /locus_tag="VIN75_17475"
     CDS             6624..>7785
                     /gene="lysA"
                     /locus_tag="VIN75_17475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY8976011.1"
                     /translation="MTTLQHPAPATFTAERLQSLAQAHATPLWVYDADTIRARVERLR
                     MFDTVRFAQKACSNTHVLRLLRGLGVKVDAVSAGEIERALHAGYDARDEHAGIVFTAD
                     EIDRPTLELVARHGIPVNAGSVDMIDQLGRHLGERASGQRVWLRINPGFGHGHSRKTN
                     TGGEHSKHGIWPSDVPAALETIARHGLRLVGLHMHIGSGVDYGHLERVCDAMVAHVQD
                     VVAHGHDVTAISAGGGLSIPYRAGDAVVDTAHYFSLWDCARRRLAAELGHPVNLEIEP
                     GRFLVAEAGALLAEVRAVKDMGANHFVLCDAGFNELVRPAMYGGFHAISVLPRANAAL
                     RAELQVDAERRPTVVAGPLCESGDVFTQDAGGVVAPRDLPPAEIGDLLVFHDT"
BASE COUNT         1158 a         2849 c         2715 g         1063 t
ORIGIN      
        1 ggtgagatcg ggccgcgtat ccgggctcgg tatgggagtg ccgtcgtaat cggtcccgta
       61 cgccacgagc ggccagccgt agttcttgcc gggctcgatc aggttgagct cgtcgccgcc
      121 gcgcggcccg tgctcgagtt cccacagccg gccgtccggt gcgaacgcga ggccgtaggg
      181 cgtgcggtgg ccggtcgtcc aggtctcggc cggcgtcagg ttggggccgt cccagacgac
      241 ggtgcgcacg accggcgcgg tcttggcggc ctccgtgtcc cgcggcggat cgatcagctc
      301 gaccgacgtc gcgccgacct tgccttccat cggattgccc ggcgcgggct tgccgtccag
      361 ggtcagccgc aggatcttgc cggccggctg gttcggatcc tgggcgggcg tgaagcgctg
      421 gcgatcgccg gccgtgagga acaggaactt gcggtccggc gagaaggcca cggccgcgcc
      481 gacctggccg cccttgccct tgaccgggtc gtgccagacc accttcaggt cttccagcga
      541 ggcagcgtcg gcgtccatct tgagcgtcgc ccgcgcgagc gccaggctcg aggtccccgt
      601 ttccgggccg ggctccgagt aggtcaggta gatcgcgcca tcgctcgcgt acgacggcgc
      661 gaggtagacg cccagcaggc cgttctgccc ctggtaatgc accgccggca cgccggcgac
      721 ctcgagcttg cggccggtct cggtcgcgat gaacagcttg cccggcttct cggtgaccag
      781 catgcggccg tcgggcagga acgcgatgcg ccagggctgg tcgaactgcg cgaccttcgt
      841 cagcttgaac ggcggcgacg gggtcggggg cagcgtcccc gcgttgatcg gcgccatcgc
      901 gggcgccgca ccgatgagca atgacaggag tccgggcttc gggaaacgca tgcgggcctc
      961 gttcgtgggt cgtcgcagca gtatcccgcg cggttcaagg cagtgcggca cgccgggcca
     1021 tcgcccggac gaccagcgaa aaacgaacta aacgaacatg ccgtcccgcc gataaacagg
     1081 acacggcatc gattcccagg aaggttcggc atggtcctcg ccccgttcaa tatccatggc
     1141 ttgcgcgcct gcgtccgcgc ggccgggttc gcgggttgag gccgtggaag agttcgacgc
     1201 cttcctccgg cgcgtcaggg acggcgtcga gcgcggcgcg ctgacgcagc aggaggccgc
     1261 gcactacgtc ggcgagcacg tcgcgcgcca gctctactgc tccagcgcga cgctgtggac
     1321 gctcgccggg ccgcccggcc tgcgcgtgct gacgcgggtc ggcggcttcg acgcgaccct
     1381 gcggatgccg ctggccgagc cgctgcagtt caccctgtcc gggccctcgg cctggcacga
     1441 cgcgctgtcg aacgagggcg tgttcgccac gaccgacacc cacgccgagc cgcgcctggc
     1501 gggcgatcac gccgccctcg ccggcctgcg ccgcgtgcgc ggcctgctgc aggcggccat
     1561 cggccgcggc gccgccccct ggggcctggt cagctgcgcc cagtacgacg ccgtccgcat
     1621 ctggacgcgg cccgagatcg tgcacctgca gcacatcgcg acggcgctgt ccgcgcgccg
     1681 cccgcgcgac cgcgcggtcg ccggcgcgcg cgaggcgccg cctgtctcgc gagcgtcaac
     1741 gtagtcttcg cgacggcgct atcctgcggg caacacccgg aggtcccatg cacatcccgt
     1801 ccctgaagga agtcgtcagc gccgaggagt ggcagctgcg ctgcgatctg gccgcctgct
     1861 accgcctcgt cgccgcgtac ggctggagcg acctcgtctt cacgcacatc agcgcgcgcg
     1921 tgcccggggg cgaccacagc ttcctgatca acccgtacgg cctgatgttc gacgagatca
     1981 ccgcgtcgtc gctggtgctg atcgaccagg aaggcaacaa gctgcgcgag tcgccgttcc
     2041 ccgtcaaccg cgccggcttc gtgatccaca gcgcgatcca cgccgcgcgc gaggacgcgc
     2101 agtgcgtcct gcacacccac acgcgcgcgg gcgtggccgt gagcgcgcag gcgggcggcg
     2161 tgctgccgat ctcgcagcag tccaccttcg tgctcgcctc gctggcgtac cacgactacg
     2221 agggcgtggc gctgcgcgac gaggagaagc cgcgcctgca ggccgacctg ggcaccgcca
     2281 actacctgat gctgcgcaac cacgggctgc tcacggtggg ccgctcgatc gccgaggcct
     2341 tcctcgcgat gtacatgttc gagagcacct gcagcatcca gctggccgcg caggccggcg
     2401 gcccgctgac gaccgtgcag cccgaggtcc tcgccggcgt ggccgagtcg atgcgcgtgc
     2461 agaccggcag ccagggcggc gcgttcgtct ggcccgcgct gctgcgaaag gtcgaacgga
     2521 tggatccggg ctaccgcacc tgagggcgtg ggcgcggcgc gacacccggc tcgggccata
     2581 ccgtgcacga aggcgctttc gcggacggga caaacccgcg agcctccccg gcgacagcga
     2641 atcgccgggc ggaccggcta tcctgccggc catcctgagg aggagttgca aaagtgaaat
     2701 gccatgcctg cgggcaggac aacgccgaca gcgcgctgtt ctgcactgcc tgccgccgac
     2761 ccctggtggc cccgaccaag gcgccgacgc gcctggaacc cctggccgcc gccgcggccc
     2821 cgtccgtcgc cgcgctgccg ccttcgccgc cgccgggcga cctgggcggc cgcaaccgct
     2881 atgcgccgcc cgagaacggc gccgcgggca agggcagccg catgggcgac gcgtccgagg
     2941 tgctgaccga cgaggaagcg cgcgccgccg tcatcggcga cgccaacacc gtctactacc
     3001 tcggccgctt cgaccgcatc gcccatggcg agggcggcgg ctggaactgg ccggcgttcc
     3061 tcatcacgtg gtactggatg ctgtaccgca agatgtgggt gcccgcgctg ctgtacttct
     3121 tcgcgccgta cgtcgtctcc atggtgctcg tcggcgtggc tgccgccgcc cccgcgctcg
     3181 gtggcgtgct ggcgctcgtc tggctggccg catggctgat cgggccgggc ctgatggcca
     3241 acggctggta ctaccggcac gtgcgcaaca agatccgcga cgtgcgcgcg cgcggcggct
     3301 cgaaggacca gatcatcgcc cgcctggagg ccgccggcgg caccagcaac atcgcggtga
     3361 tcatcgtggc cgtgctggcc attcccgcgt tcatcggcat cctggccgcg gtctcgctgc
     3421 cggcctacca gacctacacg gtcaaggcca aggtggccga tgccatcatc gagagcaccg
     3481 ccgtcgcgac ggtcgccgga cgccagttcg aggccaccgg ccagtacccg accgacatcg
     3541 accgcatcgc gcagcaggtg ccgcagcagt cgaagtacgt gcgcggcttc gagatcgacg
     3601 gcatgtccgg caccatcacc gtgcacatcc aggcgacgcc cgccgtcgac ggctcgatcc
     3661 agctcacgcc gtacgtcgac ggcaaccgcc acgtcacgtg gtcgtgctcg acgaccgacc
     3721 tgctgaagta cgtgcccaag agctgccgcg gctgacgcgc cgcgcggcgc tcaggccatc
     3781 ggcacccgca ccgcgcgcag gaacttgccc gccacgcgca tcaccgccgt ggcgcccgcc
     3841 tcgctcgcat agacgtcggg cacgcacacg gccatgccgc gcgcggtgag cgtgccgggg
     3901 tggcggatgc ggaagaacgg cgccggcggc tcgaacgcct tcgggaacgc gcggatcagc
     3961 gcgatgtcgg tgcccgggcc atggtcggcg atgctgccgg cgggcagctc gcagctggcg
     4021 agcacgtgca tcgcgaagaa cacgccgtag ccggccacca tgacgggcag gaactccagc
     4081 tcctggaagt gcgacagctc gtcgcgcacg ccctgcgaca cgacgtgcgc ggtcggatgg
     4141 aacagcaccg ccgtcggact catctcggaa cgatagagct cgagcgccgg cggcatccag
     4201 cgctcggcca gcggcgccgt ggtgcgcagc agcggctcgt tccacgcgga gtgccggtcg
     4261 tcgacccact ccgccgcggc atccgtgccg tcgatgaaga cgggcttgat ctcgaagacg
     4321 ggcatgaccc agtgtagggc catgagcccg cgcggcgttc cgtcgaggat tccgcacccg
     4381 ccgccctccg gagtcgccca gggacaacac ccgcgcagtc ggcttttttc ctgggtagga
     4441 tatgtctcga gcgacggtca ttgccgcgat gccgtccgca gaacccgaaa ggacactgat
     4501 gatcaccaaa gccctgctgt ttcgcttcga gtcgcagacc gacgaggaca cgatggacac
     4561 gttcctcgaa gacctcgcca ccgacgtctc atgggagaag gccacgcagg cgtggttcgg
     4621 catccgctac atgcgcggcg agttcggcat gtactcggtc tacgacgacg aggccggccg
     4681 cgaggcgcac ctgcagggcg atccggccag gaaggtgttc gccgccgagc acaagctgat
     4741 gaccgcggcg ccgcgcgtca cgaagatcga gctcctgggc tgcaagatgc ccaacgcgct
     4801 cgcgggcgtc accaagggcc tggtgctgcg cttcaaggcc aagcccggca aggaagccga
     4861 ggtcgccaag atgatgcgcg actgcgtgcc gctggtcgag aaggaagccg gcacgctcgc
     4921 ctggttcgcg aaccagtacg acgagagcca cttcggcacc ttcgcagcgt tcgccgacac
     4981 cggcgcccgc ttcgcccacc tcaccggcca gatcccgcgc gagctgggca tcaccggcct
     5041 gtcgctgctg ggcggcacgc ccgaggtgca catggtggac gtgatggccg gcaccgcccg
     5101 ccagcgctga gaagccgccc tgcgcggcgc gtcacgcgcg ggcgcgccct cgcgtcgatc
     5161 aggccgaggc gcccgagccc gatgccgtcc ggtcgatgaa gcgcgccagc tcgatgtcct
     5221 tcatcgtcag gcccttggcg tcgtgcgagg tcagcgtcac gtcgaccacg ttgtacgagt
     5281 tcgaccagtc gggatggtgg tcgcgccgct cggcggccag cgccacgcgc gccatgaagg
     5341 cgaacgcctc gttgaagtcg gcgaattcga gatgccgcga gatggacgac gccttcgagt
     5401 cgagcgacca gcccggaagc tgggacagca acccgggcag ctgggcggaa tcgagcttgg
     5461 cggtcatgcg gggcctttcg ggggctgcga aggcagcgat ggcctcactc tagggcctgg
     5521 ggccggccct cgccacgtcg cgggttctcg gctcgccaac gagcgcttcg ctggtcgcga
     5581 actcaaccgc ggcgaccgcg cgccgggcgc tcccaggcgc gcgcgatcgc ctcgcgcagc
     5641 gcgtcgcatt cggcctgcag cgccgtctcg aagcgcgcca ccagcgggtt gccgggccga
     5701 tgcgccggtc gcacggaata cacgcggaac gggatggcga acgccagcgg ccgcacggcg
     5761 atgcccgact ccggcccgga cagcgcgagc gccgtcagcg gattgacgat cgccagcccc
     5821 aggccgtgcc gcaccatcgc gcacacggcc accgcgctgt cggtctccac ggccagttgg
     5881 cggtcgacgc cggcctcggc gaacaccttg tcgacccact gccggtagcg gtcgcgcggg
     5941 tggaagctga cgaaggcctg gccggcgaag tcggccgcgg ccaggcgcct gcgggccagc
     6001 aacggatggc ccgccggcag cacggccacc tcgtcgagct ccagcagcag cgacggctgc
     6061 gcgccggcca gcgcgctgtc atgctcggtc acgcccaggt cgtgcgcctg cgcggccagg
     6121 cgctcctcca gcagcggcga ttcgtacggg ttgaccgaca cgctcacgcc cggatgcgcc
     6181 gcctggaagc gcgcgcaggc gcccggcagc aacgcgtgcg agaacgccgg caggcaggcg
     6241 agcgacagct gcccttcggt gaactggcgc aggcgcagcg cggtgtcggc cacgcgctcc
     6301 aggcccgcat acgagcgctg cacctcgtcg aacagcgcat aggcctgcgc ggtgggctgc
     6361 agccggccgc gcacgcgctc gaacagcgcc agccgcagca actgctccat gcgcgacagc
     6421 tcgcggctga tggtgggctg cgacgtgaac agcgccgcgg ccgcaccggt gacgctcccg
     6481 gcggtcatca cggcgcggaa cacctcgacg tggcggtggg tcaggagcgg tgcggcgggc
     6541 atcgcacgac catatcaaaa gtgaatcaga tcggcaaatg gaagcattgg actgatatgc
     6601 cgatcgcgcg caccatggca gtcatgacga cgctccagca ccccgccccc gccacgttca
     6661 ccgccgagcg cctgcaatcg ctcgcgcagg cccatgccac gccgctgtgg gtctacgacg
     6721 ccgacaccat ccgcgcccgc gtcgagcgcc tgcgcatgtt cgacaccgtg cgcttcgcgc
     6781 agaaggcctg ctccaacacg catgtgctgc gcctgctgcg cgggctcggc gtgaaggtcg
     6841 atgccgtgtc ggccggcgag atcgagcgcg cgctgcacgc cggctacgac gcccgcgacg
     6901 agcacgccgg catcgtcttc acggccgacg agatcgaccg cccgacgctg gagctcgtcg
     6961 cacgccacgg catccccgtc aacgccggat cggtggacat gatcgaccag ctgggccgcc
     7021 acctcggcga acgggcgagc ggccagcgcg tctggctgcg catcaaccct ggcttcggcc
     7081 atggccacag ccgcaagacc aacaccggcg gcgagcacag caagcacggc atctggccgt
     7141 ccgacgtccc cgccgcgctg gagaccatcg cgcgccacgg actgcggctc gtcggcctgc
     7201 acatgcacat cggctcgggc gtggactacg gccacctcga gcgcgtgtgc gacgcgatgg
     7261 tggcgcacgt gcaggacgtg gtcgcgcacg gccacgacgt cacggccatc tccgccggcg
     7321 gcggcctgtc catcccgtac cgcgccggcg acgcggtggt cgacaccgcg cactacttca
     7381 gcctgtggga ctgcgcgcgc cgccgcctcg ccgcggagct gggccatccg gtgaacctgg
     7441 agatcgagcc cggccgcttc ctcgtggccg aggcgggcgc gctgctggcc gaggtgcgcg
     7501 ccgtcaagga catgggcgcc aaccacttcg tgctgtgcga cgccggcttc aacgagctgg
     7561 tgcgccccgc gatgtacggc ggcttccacg cgatcagcgt gctgccgcgc gccaacgccg
     7621 cgctgcgcgc cgagctgcag gtcgacgccg agcgccgtcc caccgtcgtc gccggtccgc
     7681 tgtgcgaatc gggcgacgtg ttcacgcagg acgccggcgg cgtggtcgca ccgcgcgacc
     7741 tgcccccggc cgagatcggc gacctgctgg tcttccacga caccg
//
LOCUS       DATCRO010000638         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_231842, whole genome shotgun sequence.
ACCESSION   DATCRO010000638 DATCRO010000000
VERSION     DATCRO010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231842"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2615
                     /locus_tag="VIN75_17480"
     CDS             <1..>2615
                     /locus_tag="VIN75_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8976012.1"
                     /translation="KAPRLVAVRKAFALAAQEPVDYEHEALGRYDPALIPLARRRVEA
                     LKESWSSATAGQSGLASSLREQASLVADSVRKLHPEGDHMARALDAAVTQVADGAAVP
                     SPTLAMEVATSLLCLDAALDDVDIADASFGTRIHQLAERIDLARQGRTPGPIEPWMED
                     LYRRASDRQTMGSVVQELRSSLADVEGQLDKFNRNPAERETLVPVPAKLSAMRGVLSV
                     LGLDQAGQAVVRMRDEVDTVLAQSREGAEASPAERALSEQAVGRLAANVGALGFLIDL
                     LGVQPQLAKSLFKFDPESGRLETTMGRVDEIVPTEVDAPAADESELLEQVQQLAATAG
                     SDAASKAEVMQKLEELAQQATASDEKSIADAAKAARTALARSDESAATEVRQEVAKAM
                     TEFVAPPPPAPAPAPAAVSDTATAEDDEMLEVFFEEAEEVIAGARESLDKLAEDPQHF
                     ESISTVRRAFHTLKGSSRMVGLKDFGEAAWACEQLYNARLADAVIEASPDLRSFSMNV
                     LDELEGWVKALAAKNAHGYTSAPIVAAANALRDGVDPDAEPVKPKAAAPVAAPAVEAT
                     AVLPPMPSPEVSFEPAPQEASDTEILEDFANTAPPELADLPQVTESSHEAAFTLDLPS
                     DAPADKAHAPTEYLDERTVVQPLERRPEFAAPDFQFDLGELDKPAVPHVVEEEHDTGP
                     ETVPTHLGELDLLPTAHVVEPAANDAVADQMPADDAENYKVIGPLKISLSLFNIFLNE
                     ADEQSRRLCTALAEWVLELPRAVGEEAVALAHSLAGNSATVGHTELSSLARRLEHALE
                     VSQSRLVTPDEARLYSDVAEEIRRLLHQFAAGFLQTPSQPLIARFDAFQSSIQAQAAQ
                     AAHDEAVQRDRDAQA"
BASE COUNT          395 a         1005 c          905 g          310 t
ORIGIN      
        1 ccaaggcgcc ccgcctggtg gccgtgcgca aggccttcgc cctcgccgcg caggagccgg
       61 tcgactacga acacgaggcg ctgggccgct acgaccccgc gctgatcccg ctggcgcgcc
      121 gccgcgtcga ggcgctcaag gaatcgtggt cgtcggccac ggccggccag tccggcctcg
      181 cgtcgagcct gcgcgagcag gcctcgctgg tggccgactc cgtgcgcaag ctgcatcccg
      241 aaggcgacca catggcccgg gcgctcgacg ccgcggtcac gcaggtggcc gacggcgccg
      301 ccgtgccgtc gccgacgctg gcgatggaag tcgccaccag cctgctgtgc ctggacgcgg
      361 cgctcgacga cgtcgacatc gccgacgcct cgttcggcac ccgcatccac cagctggccg
      421 agcgcatcga cctcgcgcgc cagggccgca cgccgggccc gatcgagccg tggatggaag
      481 atctctaccg ccgcgcctcc gaccgccaga ccatgggcag cgtcgtgcag gagctgcgtt
      541 cgtcgctggc cgacgtcgaa ggccagctcg acaagttcaa ccgcaacccg gccgagcgcg
      601 agacgctggt gccggtgccg gccaagctgt cggccatgcg cggcgtgctg tccgtgctgg
      661 gcctcgatca ggccggccag gccgtcgtgc gcatgcgcga cgaggtcgac accgtgctgg
      721 cgcagtcgcg cgagggcgcc gaagcctcgc cggccgagcg cgcgctgtcc gagcaggccg
      781 tgggccgcct ggccgccaac gtcggcgcgc tgggcttcct gatcgacctg ctgggcgtgc
      841 agccgcagct ggccaagtcg ctgttcaagt tcgaccccga atccggccgc ctcgagacca
      901 cgatgggccg cgtcgacgag atcgtgccca ccgaggtgga cgcacccgcc gccgacgaga
      961 gcgagctgct cgagcaggtg cagcagctgg ccgccacggc cggcagcgac gccgcctcca
     1021 aggccgaggt catgcagaag ctcgaggagc tggcccagca ggccaccgcc tcggacgaga
     1081 agagcatcgc cgacgccgcc aaggccgcgc gcaccgcgct ggcccgcagc gacgagagcg
     1141 ccgccaccga ggtgcgccag gaagtcgcca aggcgatgac cgagttcgtc gccccgccgc
     1201 cgccggcccc cgcgcccgcg cccgccgccg tgtccgacac ggccacggcc gaggacgacg
     1261 agatgctcga ggtcttcttc gaggaggccg aggaagtcat cgccggcgcc cgcgagagcc
     1321 tggacaagct ggccgaggac ccacagcact tcgaatcgat ctccacggtc cgccgcgcgt
     1381 tccacacgct caagggcagc tcgcgcatgg tgggcctcaa ggatttcggc gaagccgcct
     1441 gggcctgcga gcagctgtac aacgcgcgcc tggccgacgc cgtgatcgag gccagcccgg
     1501 acctgcgcag cttctccatg aacgtcctcg acgagctcga gggctgggtc aaggcgctcg
     1561 ccgccaagaa cgcgcacggc tacaccagcg cgccgatcgt ggccgccgcc aacgcgctgc
     1621 gcgacggcgt cgacccggat gccgagccgg tcaagccgaa ggctgccgcg cccgtggccg
     1681 cgccggccgt cgaggccacg gccgtcctgc cgccgatgcc gtcgcccgag gtctcgttcg
     1741 agcccgcgcc gcaagaggcg agcgacaccg agatcctcga ggacttcgcc aacaccgcgc
     1801 cgcccgagct cgccgacctg ccgcaggtca ccgagtcgtc gcacgaggcc gcgttcacgc
     1861 tggacctgcc gtccgacgcg cctgccgaca aggcccacgc gccgaccgag tacctcgacg
     1921 aacgcaccgt ggtgcagccg ctcgagcgcc gtcccgagtt cgccgcgccg gacttccagt
     1981 tcgacctggg cgagctcgac aagccggccg tgccgcacgt cgtcgaggaa gagcacgaca
     2041 cggggcccga gaccgtgccg acgcacctgg gcgagctcga cctgctgccc accgcgcacg
     2101 tcgtcgagcc ggccgccaac gatgccgtgg ccgaccagat gccggccgac gatgccgaga
     2161 actacaaggt catcgggccg ctgaagatca gcctgagcct gttcaacatc ttcctgaacg
     2221 aagccgacga gcagtcgcgg cgcctgtgca cggcgctcgc cgaatgggtg ctggagctgc
     2281 cgcgcgcggt cggcgaggag gccgtcgctc tggcccattc gctggccggc aactcggcca
     2341 ccgtgggcca caccgagctg tcctcgctgg cgcgccgcct cgagcacgcg ctggaggtgt
     2401 cgcagtcgcg cctggtgacg ccggacgagg cgcgcctgta cagcgacgtg gccgaggaga
     2461 tccgccgcct gctgcaccag ttcgcggccg gcttcctgca gacgccgtcg cagccgctca
     2521 tcgcgcgctt cgacgcgttc cagtcgtcca tccaggcgca ggccgcgcag gccgcgcacg
     2581 acgaggccgt ccagcgcgac cgcgatgccc aggcc
//
LOCUS       DATCRO010000639         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_243339, whole genome shotgun sequence.
ACCESSION   DATCRO010000639 DATCRO010000000
VERSION     DATCRO010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243339"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VIN75_17485"
     CDS             complement(<1..1167)
                     /locus_tag="VIN75_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8976013.1"
                     /translation="MKNPLLDSPGLPAFDDIRPETVTPAMDALLKQADAALERVVGPD
                     VPPDFDAMSSVFTVATERLSRAWRAVEHLTGVADTPELRAAHAENLPRITEFFTRQGA
                     DERLYAKYRAVSARADADQLGPVRRKALANWLRDFKLGGADLRGAAKARYAAIQERLA
                     ELSRQFSEHVLDATDGYALYVPEADLAGVPADAIAAARAGAEADGRAGTCKLTLHFPS
                     YLPVMQMARNRALREKLYTAYVTRASEAGPAERDNTPVIRELLTLRQEEAELLGYRNF
                     AELSLVPKMAGSPAEVIAFLRDLGRRARPSAEADLAELREFAAQELGIGQLQAWDVPF
                     ASERLKEARYAFSDEEVRQFFTLPKVLDGLFRIIETLFEVRIVKSAAPAWHESVT"
     gene            complement(1330..2181)
                     /gene="folD"
                     /locus_tag="VIN75_17490"
     CDS             complement(1330..2181)
                     /gene="folD"
                     /locus_tag="VIN75_17490"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY8976014.1"
                     /translation="MTAQLIDGVALSRQIRAEVAQRAAALTARGHQPGLAVILVGDDP
                     ASATYVRNKVKACADAGVRSVLEKYEATLTEAELMARLDALNADPTIHGILVQMPLPR
                     HIDPQKVIERISTAKDVDGYSVQSAGDLMAGLPGFRPCTPYGCMKLIESTGVPIKGKH
                     AVVIGRSNTVGKPMALLLLQANATVTICHSATADIGAHTRQADIVVVATGRRDTLRGD
                     MVKPGAIVIDVGINRNDEGKLCGDVDFASVAPLASFITPVPGGVGPMTITMLLANTIE
                     SAERVAG"
     gene            complement(2346..2975)
                     /locus_tag="VIN75_17495"
     CDS             complement(2346..2975)
                     /locus_tag="VIN75_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976015.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVKCFDSAESFL
                     SRFDPREVACVIADIRMDGMSGLELQDRLIERKSPLPIVFVTGHGDVPMAVSTMKKGA
                     MDFIEKPFKEDDLVSLVERMLDQARSAFSQHQEQASRDALLQRLTTREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGAPSNKQA"
BASE COUNT          427 a          998 c         1079 g          500 t
ORIGIN      
        1 cgtcacgctc tcgtgccagg ccggcgccgc cgacttgacg atcctgacct cgaacagcgt
       61 ctcgatgatg cggaacaggc cgtccagcac cttgggcagc gtgaagaact ggcgcacctc
      121 ctcgtcgctg aacgcgtagc gcgcctcctt cagtcgctcg ctggcgaacg ggacgtccca
      181 ggcctgcagc tgaccgatgc cgagttcctg cgcggcgaac tcgcgcagtt cggcgagatc
      241 ggcctcggcg ctgggacggg cgcggcgtcc gagatcgcgc aggaaggcga tgacctccgc
      301 cggcgagccg gccatcttgg gcaccagcga cagctcggcg aaattgcgat agccgagcag
      361 ctcggcttct tcctggcgca gcgtcagcag ctcgcggatg accggcgtgt tgtcgcgctc
      421 ggccggcccc gcctcgctcg cgcgcgtgac gtaggcggtg tacagcttct cgcgcagcgc
      481 gcggttgcgc gccatctgca tgacgggcag gtagctcggg aaatgcagcg tcagcttgca
      541 ggtgcccgcg cggccgtccg cctccgcgcc cgcgcgcgcg gccgcgatcg cgtccgcggg
      601 cacgcccgcg aggtcggcct cgggcacgta cagggcatag ccgtcggtgg cgtcgagcac
      661 gtgctcggag aactggcgcg acagttccgc gagccgttcc tggatggcgg catagcgcgc
      721 cttggccgcg ccgcgcaggt cggcgccgcc cagcttgaag tcgcgcagcc agttggccag
      781 cgccttgcgc cgcacggggc cgagctggtc cgcgtccgca cgcgcagaga cggcgcggta
      841 cttggcgtac aggcgttcgt cggcgccctg gcgcgtgaag aactcggtga tgcgcggcag
      901 gttctcggcg tgcgcggcgc gcagctccgg tgtgtcggcg acgccggtca ggtgttccac
      961 cgcgcgccag gcgcgcgaca gccgctcggt ggcgacggtg aacacggacg acatggcgtc
     1021 gaagtcgggc gggacatcag ggccgacgac gcgctccagc gccgcgtcgg cctgcttgag
     1081 cagcgcgtcc atcgccgggg tgacggtctc cggtcggatg tcgtcgaagg cgggcaggcc
     1141 ggggctgtcg agcaaggggt tcttcatggt gtcgggcgtc aggggcatcc ggcgcagatg
     1201 gtgtccgggc cgggcgttgc aagactcaag ggtgggcgtc gcctcgaatg tacccctcgt
     1261 ggcggcgaac tccaggggaa acaacagggc gaaatcaccc gttccgcgcg gtgcgggcga
     1321 cgcccggcct cagccggcca cgcgctccgc cgactcgatc gtgttggcca gcaacatcgt
     1381 gatcgtcatc ggcccgacgc cgccaggcac cggcgtgatg aacgacgcca gcggcgccac
     1441 cgacgcgaag tccacgtcgc cgcacagctt gccttcgtca ttgcgattga tgccaacgtc
     1501 gatgacgatg gcgcccggct tgaccatgtc gccgcgcagc gtgtcgcggc ggccggtggc
     1561 cacgacgacg atgtcggcct ggcgggtgtg tgcgccgatg tcggcggtgg cactgtggca
     1621 gatcgtgacg gtggcgttgg cctgcagcag cagcagggcc atcggcttgc cgacggtgtt
     1681 gctgcggccg atcacgaccg catgcttgcc cttgatcggc acgcccgtgc tctcgatcag
     1741 cttcatgcag ccgtacggcg tgcaggggcg aaagcccggc aggccggcca tcaggtcgcc
     1801 ggcgctctgc accgagtagc cgtcgacgtc cttggcggtc gagatgcgct cgatcacctt
     1861 ctgcggatcg atgtgcctgg gcagcggcat ctgcaccagg atgccgtgga tcgtcgggtc
     1921 ggcgttgagc gcgtccaggc gcgccatcag ctcggcctcg gtgagcgtgg cctcgtactt
     1981 ctcgagcacc gagcgcacgc ccgcgtcggc gcaggccttg accttgttcc tgacgtacgt
     2041 cgccgaagcc ggatcgtcgc ccacgaggat gaccgccagg cccggctggt ggccgcgggc
     2101 ggtgagggcg gcggcgcgtt gcgcgacttc ggcgcggatc tgcctggaca gcgcgacgcc
     2161 gtcgatcagt tgtgcggtca tgggcgagct tcctttgcga ttgatattca aaatgcaaga
     2221 ggccgcttcg tcagcggcct ctcatcggca tgggtgtcaa ctggcgggcg agcgctcggc
     2281 cgcccggagc gagcccgctg agccgctcgg gggcggcgag cggaggcgag cccaggccgt
     2341 catgactagg cttgcttgtt ggagggggcg cccagcgcga tcttgagcag gtcggccacc
     2401 gtgttggcgt tgagcttctc catgatgttg gcgcggtgcg cctccacggt cttgatgctg
     2461 atgcccaggt cgtcggcgat ctgcttgttc aggcggccgg cgacgatgcg ctcgagcacc
     2521 tgcgcctcgc gcgtggtcag acgctgcagc agcgcgtcgc ggctggcctg ttcctggtgc
     2581 tgcgagaacg ccgagcgggc ctggtcgagc atgcgctcca cgagcgagac gagatcgtct
     2641 tccttgaacg gcttctcgat gaagtccatc gcgcccttct tcatcgtgga caccgccatc
     2701 ggcacgtcgc cgtggccggt gacgaagacg atgggcagcg ggctcttgcg ctcgatcagg
     2761 cgatcctgca gctccaggcc gctcatgccg tccatgcgga tgtcggcgat gacgcacgcc
     2821 acctcgcgcg ggtcgaagcg cgacaggaag gattcggcgg aatcgaagca cttgacgcgg
     2881 tagtccttgc cctcgagcaa ccactgcagc gaatcgcgaa ccgcctcgtc atcgtcgacg
     2941 acgtagacgg tacctttctt ggggatcagg ctcattgacg caaaactccg tgcttcgcac
     3001 tttc
//
LOCUS       DATCRO010000640         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_918639, whole genome shotgun sequence.
ACCESSION   DATCRO010000640 DATCRO010000000
VERSION     DATCRO010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918639"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIN75_17500"
     CDS             complement(<1..305)
                     /locus_tag="VIN75_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEY8976016.1"
                     /translation="MDPSSQDLVVARPEGLYCPAGDFFIDPWQPVAHALVTHAHGDHL
                     RPGHGHYLTAEAGRHVVASRVGAVDLQTLAYGEAITHRGVRVSFHPAGHVLGSAQIRL
                     "
     gene            457..1680
                     /locus_tag="VIN75_17505"
     CDS             457..1680
                     /locus_tag="VIN75_17505"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976017.1"
                     /translation="MSAPSTPTAVDDGGRLAALTSLRFVAAFSVLVLHYRDLFGPMPG
                     WVFKGIVGGQYGVPFFFVLSGFILTWRYHGWFADGVTDARFWRFQRFRLARIYPVYVV
                     GLLLDTPWHLYERAGQLGDMGQTFWASWLLNLIGLQAWVPAMPFAMFWNTPAWSVSAE
                     FFFYATFPFACTWLTRRVRSTAGLATLLAAVAVGGIALYYAVIWLVYVVFPLSGETQY
                     IVLVYNPLLRYSEFLAGCIAGQIFLRWRARAVPPSSPADLARARRRRDAVIVVCLLAV
                     CVRIWMPDYTGPSQALWTLDYAVKYPVFILPFVAIILAIASGPTFLSPLLERPWMVLL
                     GESSYALYMIHWSATSFLRLHWLGEWSTPPVHVAFLLATVGASVLLYRRFELPWRERL
                     RGRRETAGALVPAAT"
     gene            complement(1709..2962)
                     /locus_tag="VIN75_17510"
     CDS             complement(1709..2962)
                     /locus_tag="VIN75_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8976018.1"
                     /translation="MHSFLFDDAAFAEAIDAIPLVSRDVSSDESYWNAVRGLYATTAS
                     LSNLENGYWGVMTEPVKDVYRRWTDRVNHDNTVFIRRQWVDASEGVRATLADALGCGV
                     DEVALTRGATEAMLALISGYNALQPGDTVLYSDLDYPAMRHAMAWLRDRRGVEPVHFA
                     IPEPADRDSVLDAYRRMLERHPRTRLVLLTHLSHCTGLVLPVRELSAMARAAGADVVV
                     DAAHSWGQMDFDIADLDAPFVAINLHKWIGAPLGCGALYIRKDRLGTIDPYFGDRDHP
                     EDDVRSRIHTGSPNFAAWLSVPSALALHRRIGARNKEARLRHLRNSWVSAARGMAGVE
                     VLTPDDAAMVAGITAFRLAGATTSEQNEAIVTTLRDRHRVLTVRRDGPARGDAVRVTP
                     SLYTRPQDVERLVAGLRALTGGAAG"
BASE COUNT          402 a         1042 c         1084 g          484 t
ORIGIN      
        1 agccggatct gcgccgagcc cagcacgtgg ccggccggat ggaacgacac gcgcacgccg
       61 cgatgcgtga tcgcctcgcc gtacgcgagc gtctgcaagt cgaccgctcc gacgcggctc
      121 gccacgacat ggcgcccggc ctcggccgtc aggtagtggc catgccccgg ccgcaggtgg
      181 tcgccgtgcg cgtgcgtgac gagcgcgtgc gcgacgggct gccacgggtc gatgaagaag
      241 tcccccgccg ggcagtacag gccttccggg cgcgcgacga cgaggtcctg ggacgacgga
      301 tccatgcggg ataccggaaa ccggtgaggc ggcggtcgag caatcgatgt tcccggagtg
      361 ccggatgcgt gagaataccc tttgcgatcc acatggagac cagatgcagg ccatttcctg
      421 gcgcgggcgg cgccgcgcgg cgaggggttg cgcacgatga gcgcgccctc cacccccacc
      481 gcggtcgacg acggcggccg gctcgcggcg ctgaccagcc tgcgcttcgt cgcggcgttc
      541 tccgtgctgg tgctgcacta ccgcgatctg ttcgggccga tgcccggctg ggtcttcaag
      601 ggcatcgtgg gcggccagta cggcgtgccc ttcttcttcg tgctgagcgg cttcatcctc
      661 acgtggcgct accacggctg gttcgcggac ggcgtcaccg acgcccgctt ctggcgcttc
      721 cagcgtttcc gcctggcgcg catctacccg gtctacgtcg tcggcctgct gctggacacg
      781 ccgtggcacc tctacgagcg cgcaggccag ctcggcgaca tgggccagac cttctgggcg
      841 tcgtggctgc tgaacctgat cggcctgcag gcctgggtgc cggcgatgcc gttcgcgatg
      901 ttctggaaca cgcccgcctg gagcgtgtcg gccgaattct tcttctatgc gacgttcccg
      961 ttcgcctgca cgtggctgac gcggcgggtg cgcagcaccg cgggcctcgc gacactcctg
     1021 gcggccgtgg cggtgggcgg catcgcgctc tactacgcgg tgatctggct ggtgtacgtc
     1081 gtgttcccgc tcagcggcga gacgcagtac atcgtgctcg tctacaaccc gctgctgcgc
     1141 tacagcgagt tcctggccgg ctgcatcgcg gggcagatct tcctgcggtg gcgcgcgcgg
     1201 gccgtgccgc cgtcgtcgcc cgcggacctc gcccgcgcaa ggcgccgccg cgacgcggtg
     1261 atcgtcgtgt gcctgctggc ggtgtgcgtg cgcatctgga tgccggacta caccggtccg
     1321 agccaggcgc tgtggaccct ggactacgcg gtcaagtacc cggtcttcat cctgccgttc
     1381 gtggcgatca tcctggccat cgcgtcgggc ccgaccttcc tcagcccgct gctggaacgg
     1441 ccgtggatgg tgctgctggg cgagtcgagc tatgcgctgt acatgatcca ctggtcggcc
     1501 acgtcgttcc tgcgcctgca ctggctcggc gaatggtcga cgccgccggt gcacgtcgcc
     1561 ttcctgctgg cgacggtggg cgcgtcggtg ctgctgtacc gccgcttcga gctgccgtgg
     1621 cgcgagcgcc tgcgcggccg gcgcgagacg gcgggagcgc tggtgccggc ggccacgtga
     1681 aggcgactgg cggtgcgagt cgggactcct agccggcagc cccgcccgtc aacgcacgca
     1741 atccggcgac gagacgctca acgtcctgcg gccgcgtgta cagcgacggc gtgacacgca
     1801 cggcgtcgcc gcgggcgggc ccgtcgcggc gcaccgtcag cacgcgatgc cggtcgcgca
     1861 gcgtggtcac gatggcctcg ttctgctccg aggtggtcgc gcccgcgaga cggaaggccg
     1921 tgatgccggc caccatcgcc gcgtcgtcgg gcgtcaggac ctcgacgccc gccatcccgc
     1981 gcgccgccga cacccacgag ttgcgcaggt ggcgcagccg cgcctccttg ttgcgcgcgc
     2041 cgatccgccg atgcagcgcg agcgccgacg gcacgctgag ccaggccgcg aagttgggag
     2101 aaccggtgtg gatgcgcgag cggacgtcgt cctccggatg gtcgcggtcg ccgaaatacg
     2161 ggtcgatcgt gcccaggcgg tccttgcgga tgtacagcgc gccgcaaccc agcggggcgc
     2221 cgatccactt gtgcaggttg atcgcgacga agggcgcgtc gaggtcggcg atgtcgaagt
     2281 ccatctggcc ccaggaatgc gcggcgtcca cgacgacgtc cgccccggcg gcgcgcgcca
     2341 tcgcggacag ctcgcgcacg ggcaacacga ggccggtgca gtggctcagg tgcgtgagca
     2401 gcaccaggcg tgtgcgcgga tggcgctcga gcatccgacg atacgcgtcg agcacgctgt
     2461 cccgatccgc cggctccggt atcgcgaagt ggacgggctc gacgccgcgc cggtcgcgca
     2521 gccaggccat ggcgtggcgc atggccgggt agtcgaggtc gctgtacagg acggtgtcgc
     2581 cgggttgcag cgcgttgtag ccgctgatga gcgcgagcat cgcctcggtc gcgccgcggg
     2641 tgagcgcgac ctcgtcgacc ccgcagccga gcgcgtcggc gagcgtggcc cgcacgccct
     2701 cgctggcgtc gacccactgc cggcggatga agaccgtgtt gtcatgattg acgcgatcgg
     2761 tccagcgccg gtagacatcc ttgacgggtt ccgtcatcac gccccagtac ccgttctcga
     2821 ggttggacag cgaggcggtc gtggcgtaga gcccgcgcac cgcgttccag tacgactcgt
     2881 cggaactgac gtctcgcgac accagcggga tcgcgtcgat cgcctcggcg aatgccgcat
     2941 cgtcgaacag gaaggagtgc atggagaggg ctcgatgtca gtgaccattc gattctcccg
     3001 catgcggacg ac
//
LOCUS       DATCRO010000641         9262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_895878, whole genome shotgun sequence.
ACCESSION   DATCRO010000641 DATCRO010000000
VERSION     DATCRO010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895878"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..615
                     /locus_tag="VIN75_17515"
     CDS             145..615
                     /locus_tag="VIN75_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976019.1"
                     /translation="MIPSRIRLLVVSSLLLAAAAGAATVKPPKEAAFGTRDQLRACLD
                     QDDAMKARRRDLEAAAAEHNRKFDANEAEDARLAEMKARLDRSDKDAIAAFNKAVTEH
                     AQHTRDLNQEAEAQEAGMKSLEADKADMDDKCGSLSYRPADIDAVNKERKKAAS"
     gene            741..1250
                     /locus_tag="VIN75_17520"
     CDS             741..1250
                     /locus_tag="VIN75_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976020.1"
                     /translation="MPALRLLALAAATLACSAAVATTLSTGKPAGPQAGTSGLATRDQ
                     LRDCMMTEAGLKQRFEALQATSAAHEKMAAQVEAESDHLAELQAKLDHDSSTAIKAFN
                     SAVDEHNRHVAELNKDARDSDPASHAYNEDMAAFNHRCAKLRYSVDDMDAVMTERKKA
                     AAAAAAASR"
     gene            complement(1257..2099)
                     /locus_tag="VIN75_17525"
     CDS             complement(1257..2099)
                     /locus_tag="VIN75_17525"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY8976021.1"
                     /translation="MAAPLADFDLACRAAALYVYPIKACAGVAVPEIVLDARGGAVGD
                     RGWAIVDAEGAVTWLGSHPRLALVQPAFEGAGLALAAPDVAPVATPVAMAPRQVKIWS
                     DVHRCHEVFEAEDAGDAVAAWLECVTGAPLRLVKLEDAVHARDGNNALHLVFTPSVAA
                     VAARWDAGGGAPVDVRRFRPNVVLALPEDGNEFVEESLEALAWQRAGDGQTRLDVTAP
                     CIRCVVPNVDPASAQVDASLLDTLAGLSLQRRPGGTVFGIYARGAAGARLRVGDVARM
                     MPAF"
     gene            2233..3525
                     /locus_tag="VIN75_17530"
     CDS             2233..3525
                     /locus_tag="VIN75_17530"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8976022.1"
                     /translation="MTVRVLTGITTTGTPHLGNYVGAIRPAIAASRRADVESFLFMAD
                     YHALVKCDEPARVEESRLALAATWLAAGLDPERVVFYRQSDIPEIPELNWLLTCVTPK
                     GMMNRAHAYKAAVDANAGGEPDDGINMGLFCYPVLMSADILMFNAHQVPVGKDQVQHI
                     EMARDIAQRFNHLFGQGQALFTLPEAVTEAEMQLLPGLDGRKMSKSYDNAIPLFDGGA
                     KGLSAAIARVVTDSKLPGEPKDPDTSHLVTLFDAFATPAERAAFRAELRAGLAWGEAK
                     KRLHDAIDREIGPMRAKYEQYMARPELIEEQLLIGARKARAIAGPFLQQLREAVGLRR
                     FQARAAVAAAAPKAASAALATFKQYREADGQFYFKLVGAGDTLLLQSAAFAQGRDAGV
                     WVGRFKKEGAAALAGAPVTLAADEATVRAELDRFLEVE"
     gene            3677..4084
                     /locus_tag="VIN75_17535"
     CDS             3677..4084
                     /locus_tag="VIN75_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976023.1"
                     /translation="MNYLHDHPHASRVLLVSLAAMALLVAMSAAAAAEPVTSDQFAPG
                     WQSDARPLYRVGIQHLTGRDKYYVPAILPRGDYVVIKRTGDKAEVVDYRFSVTSDNAQ
                     QYLFMEPGYGGNLEAVPLRDVPSLAQQHKSGLR"
     gene            4142..4804
                     /locus_tag="VIN75_17540"
     CDS             4142..4804
                     /locus_tag="VIN75_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEY8976024.1"
                     /translation="MRLDTLQYQTGDAPKSCVIILHGLGASATDFVPVARQWDLRALG
                     DVRYVFPFAPEQPVTINGGMRMPAWYDILGLDLVRREDEKSLRASAAALQALVDREIA
                     GGIPASRIVLAGFSQGCAMTLLTGLRAPQRLAGLLGMSGYLPLAATTAAERSQANQDV
                     PIFLGHGRMDPVVPFQRGIDTRDALVALGHDVQWRDYPMQHSVCEQEIEDTQAWLLKV
                     LG"
     gene            4809..5288
                     /locus_tag="VIN75_17545"
     CDS             4809..5288
                     /locus_tag="VIN75_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976025.1"
                     /translation="MTRQLLRWPALLVAAWVAGCATTPVDGPRPQGFDLLNEGAGDVH
                     DVQVRYGTHVFPPAGATAVVRAPTVTGQGFHQEATVAIPATAQVHWVSVDGRGHDATV
                     PIDTLIADRALFHGVQFVFVDDHLDVVFAYEREAPKPRGLDYLRTWSSAAPRLLSDP"
     gene            complement(5299..5841)
                     /locus_tag="VIN75_17550"
     CDS             complement(5299..5841)
                     /locus_tag="VIN75_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8976026.1"
                     /translation="MDDLRSLTARFPRAGRLEAIVLRPGRDAPAVPVPSARALAERGL
                     EGDHRGDKPASRPGGGKRQVTLIQAEHLPLIRAWTGMDVAPADLRRNLVISGVNLLAQ
                     HALFGDRPLQVRIGADVVIVVTGPCDPCSKMEALLGPGGYNAMRGHGGTTARVIRGGL
                     LAVGDAVVVEPGEAAQAPPS"
     gene            complement(5894..6037)
                     /locus_tag="VIN75_17555"
     CDS             complement(5894..6037)
                     /locus_tag="VIN75_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976027.1"
                     /translation="MGKEQRATKNVKKPKKDTSPPKAVSSDRPTAPVTAVAPKGKLAK
                     KPL"
     gene            6176..7243
                     /locus_tag="VIN75_17560"
     CDS             6176..7243
                     /locus_tag="VIN75_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8976028.1"
                     /translation="MPRTPMFAPLPDGPLDVVGDVHGELEALLALLGHLGYDADGGHP
                     DGRHLVFVGDLCDRGPDSPGVVDLVQRLVASGRALATLGNHEINLLRGDRKDGNDWFW
                     SEFGARDAKYEPRAHLPADQRDDVIAFLRRLPLALERSDLRVVHAAWDRASIAQLRAA
                     DPALSRVEHFRRWEAEADAQIAERGIGEAAHVEYAEYRERLVDPGQAVPMLMATAQVD
                     ELRQMGNPIRVVTSGIERVAKRPFFASGHWRFVERIRWWDEYGEDVPVVVGHYWRQFA
                     HVDRASLGKTEENLFTDLAPTAWHGLRRNVFCIDFSVGGRFLERPLPPGVLPRSRLAA
                     LRWPERVLALDTGEMLPTIGA"
     gene            complement(7382..8281)
                     /locus_tag="VIN75_17565"
     CDS             complement(7382..8281)
                     /locus_tag="VIN75_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976029.1"
                     /translation="MDRLDAMKVFLAVVDAGSLSAAGRRLGMPLATVSRKLAELEEHL
                     GARLINRSTRRLELTEPGRGYEQACRRILDDVAGAEAAAAGEYDTPRGELAVTAPLVF
                     GRLHVLPVVSEFLRANAEVDVRLALGDRIAHLIDEHVDVAVRIGALPDSRLNATRLGQ
                     LRSVVCASPAWLKAHGTPRTPDDLDAHRCITFESTFPAPWRFAGGASFTPARPRLVVN
                     TAEAAVDAAVAGLGVTRLLSYQADAALRDKRLRLLLRPFEPEPVPVHLVWDGQQRVTS
                     KLRTFLDFAAPRLRASLAALRAE"
     gene            8393..9004
                     /locus_tag="VIN75_17570"
     CDS             8393..9004
                     /locus_tag="VIN75_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8976030.1"
                     /translation="MTSTAYPSDIAFTPAVKALQQARGSRRSYSRMEQGRGWQTAIDD
                     GVRGFIEAQTTVFMATANAVGQPYIQHRGGPAGFLHVLDEHTIAFADFSGNRQFITTG
                     NLADNPKAQLFLMDYAHRQRLKIWGEARVETDPALVERLMPPGYKARAEQAIVFTVKA
                     WDANCPQHIPQLVPVDEVIPAIAERDRRIAELESEVAALKKSG"
     gene            9033..>9262
                     /gene="eutB"
                     /locus_tag="VIN75_17575"
     CDS             9033..>9262
                     /gene="eutB"
                     /locus_tag="VIN75_17575"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY8976031.1"
                     /translation="MSHRATVGHQVFGFADLRELMGKASPARSGDALAGVAAATAQER
                     VAAQAALADVPLKAFLSEAVVPYEHDEVTRLIV"
BASE COUNT         1329 a         3396 c         3314 g         1223 t
ORIGIN      
        1 gcgctgttgc agctgctcgc gtggtggcgc cggcgccgtc gctgcgcgcc cactttggca
       61 cgctgactca gggtctttgc ggagtcggcg gcgtcgcccg cggtgcgcac aattggaaac
      121 gtttcccacc gcacgcaggc cgccatgatc ccatcgcgca tccggttgct ggtcgtctcc
      181 tccctgctgc tcgccgccgc ggcgggtgcg gccacggtga aaccgcccaa ggaggccgcg
      241 ttcggcacgc gcgaccagct gcgcgcctgc ctcgaccagg acgacgcgat gaaggcgcgt
      301 cgccgcgacc tcgaggccgc cgccgcggag cacaaccgga agttcgacgc gaacgaggcc
      361 gaggacgccc ggctggcgga gatgaaggcc aggctcgatc gcagcgacaa ggacgcgatc
      421 gccgccttca acaaggccgt gacggagcac gcgcagcaca cgcgcgacct gaaccaggag
      481 gccgaggcgc aggaggccgg catgaagtcg ctcgaggccg acaaggccga catggacgac
      541 aagtgcggca gcctcagcta ccggccggcc gatatcgacg ccgtgaacaa ggaacgcaag
      601 aaggccgcct cctgacggcg cgggccggcg gtcgcgccgc gggcgatctc gtgccgtgct
      661 gcgcatttcc tcgtccggat gcggtcgcgc cgcccggcag gcggcgacaa tgcgggcctt
      721 ccgcatcctc ctggacgtcc atgcccgccc tgcgcctcct ggctctcgcc gccgccacgc
      781 tcgcctgcag cgcggccgtc gccaccacgc tgtccaccgg caagccggcc ggtccgcagg
      841 ccggcacctc gggcctggcc acgcgcgacc agctgcgcga ttgcatgatg acggaggccg
      901 gcctcaagca gcgcttcgag gcgctccagg cgaccagcgc cgcgcacgag aagatggcgg
      961 cgcaggtcga ggccgagagc gaccacctgg ccgaactgca ggccaagctc gatcacgaca
     1021 gctcgaccgc catcaaggcc ttcaacagcg cggtcgacga gcacaaccgc cacgtcgccg
     1081 agctcaacaa ggacgcgcgc gattccgacc ccgccagcca cgcatacaac gaggacatgg
     1141 ccgcgttcaa ccaccgctgc gcgaagctgc gctacagcgt cgacgacatg gatgcggtga
     1201 tgaccgagcg caagaaggcc gcggccgcgg ctgccgcggc gtcgcgctga aaagcgctag
     1261 aacgccggca tcatccgcgc gacgtcgccc acgcgcaggc gcgcgcccgc ggcgccgcgg
     1321 gcatagatcc cgaagacggt gcccccgggc cgccgttgca gcgacaggcc ggccagcgtg
     1381 tccagcaggc tcgcgtccac ctgcgcggac gccgggtcga cgttgggcac cacgcagcgg
     1441 atgcagggcg ccgtgacgtc cagccgggtc tggccgtcgc ccgcgcgctg ccaggccagc
     1501 gcctccaggc tctcctcgac gaactcgttg ccgtcctcgg gcagcgccag cacgacgttg
     1561 ggccggaaac ggcggacgtc gaccggcgcg ccgccgcccg cgtcccagcg ggccgcgacg
     1621 gccgcgacgg acggcgtgaa cacgaggtgc agcgcgttgt tgccgtcgcg ggcgtgcacg
     1681 gcgtcctcca gcttgaccag gcgcagcggc gcgccggtca cgcattccag ccaggccgcg
     1741 acggcgtcgc cggcgtcctc ggcctcgaag acctcgtggc agcggtgcac gtcgctccag
     1801 atcttcacct ggcgcggcgc catggccacg ggcgtcgcga ccggcgcgac gtccggcgcg
     1861 gcgagcgcga gcccggcgcc ctcgaacgcg ggctgcacca gcgcgagccg cgggtgcgaa
     1921 cccagccagg tgacggcgcc ctcggcgtcg acgatggccc agccgcggtc gcccacggcg
     1981 cccccgcgcg cgtccagcac gatctcgggc acggccacgc cggcgcaggc cttgatgggg
     2041 tagacgtaca gcgccgcggc gcggcaggcg aggtcgaagt cggcgagggg ggcggccatg
     2101 gcggagcgat cttgccgcag gcgccggcgc ggtgcgcggg cgggcctgtc gggtcttgcg
     2161 attctcgcgc gccgcggcgc gccggacgcc atccggcaca atccccgttc ggctttcttc
     2221 cgcacggctc ccatgactgt tcgcgtcctc accggcatca ccaccaccgg caccccgcac
     2281 ctcggcaact atgtcggcgc catccggccc gcgatcgcgg ccagcaggcg cgcggacgtc
     2341 gagagcttcc tgttcatggc cgactaccac gccctggtca agtgcgacga gccggcgcgc
     2401 gtcgaggaat cgcgcctggc gctggccgcc acctggctgg ccgcgggcct ggacccggag
     2461 cgcgtggtgt tctaccgcca gagcgacatc cccgagatcc ccgagctgaa ctggctgctg
     2521 acctgcgtca cgcccaaggg catgatgaac cgcgcccacg cgtacaaggc cgcggtcgac
     2581 gccaacgccg gcggcgagcc ggacgacggc atcaacatgg gcctgttctg ctacccggtg
     2641 ctgatgtcgg ccgacatcct gatgttcaac gcgcaccagg tgcccgtggg caaggaccag
     2701 gtgcagcaca tcgagatggc gcgcgacatc gcgcagcgct tcaaccacct gttcggccag
     2761 ggccaggcgc tgttcacgct gcccgaggcg gtgaccgagg ccgagatgca gctgctgccg
     2821 ggcctggacg gccgcaagat gtccaagagc tacgacaacg cgatcccgct gttcgacggg
     2881 ggcgccaagg ggctgtccgc ggccatcgcg cgcgtcgtca ccgactcgaa gctgcccggc
     2941 gaacccaagg atccggacac gtcccacctg gtcaccctgt tcgacgcgtt cgcgacgccg
     3001 gccgagcgcg ccgcgttccg cgccgagctg cgcgccggcc tggcctgggg cgaggccaag
     3061 aagcggctgc acgacgccat cgaccgcgag atcggcccga tgcgtgcgaa gtacgagcag
     3121 tacatggcgc ggcccgagct gatcgaggag cagttgctga tcggcgcccg caaggcccgc
     3181 gccatcgccg gcccgttcct gcagcagctg cgcgaggccg tcggcctgcg ccgcttccag
     3241 gcccgcgcgg ccgtcgccgc cgcggcgccc aaggccgcca gcgcggcgct ggccacgttc
     3301 aagcagtacc gcgaggccga cggccagttc tatttcaagc tggtgggcgc gggcgacacg
     3361 ctgctgctgc agagcgccgc cttcgcgcag ggccgcgacg ccggcgtctg ggtcggccgg
     3421 ttcaagaagg agggcgcggc ggcgctcgcc ggcgcgccgg tgacgctggc ggccgacgag
     3481 gcgacggtgc gcgccgagct cgaccgcttc ctcgaggtcg agtaagccga cgtaaagttc
     3541 cggcggcgtt gcaggacgaa tccggcgaaa cggagcaaga tcacgggtgt cggcggcgcc
     3601 ggaacggcga ttgccgcatt cctcccgtgg cagggatgtc acccgacgcc acgcagcctt
     3661 gaagcgaggt gccgtcatga actatctgca cgatcatccc cacgccagcc gggtcctgct
     3721 ggtgtccctg gccgcgatgg cgctgctcgt cgcgatgtcg gcggccgcgg cggccgagcc
     3781 ggtcaccagc gaccagttcg cgccgggctg gcagtccgac gcccgcccgc tctaccgggt
     3841 cggcatccag cacctgaccg ggcgcgacaa gtactacgtg ccggccatcc tgccgcgcgg
     3901 cgactatgtc gtcatcaagc gcaccggcga caaggccgag gtggtcgact accgcttcag
     3961 cgtcacgtcc gacaacgccc agcagtacct gttcatggag cccggctacg gcggcaacct
     4021 cgaggccgtg ccgctgcgcg acgtgcccag cctggcgcag cagcacaaga gcggcctgcg
     4081 ctgacccggc gagcgcagga tccccggggc cgcgcgccgg cgagttcggt atgctggcgg
     4141 gatgcgtcta gacaccctcc aataccagac cggcgacgcg ccgaagtcct gcgtcatcat
     4201 cctgcacggc ctgggcgcca gcgccaccga tttcgtgccg gtggcccgcc agtgggacct
     4261 gcgcgcgctc ggcgacgtgc gctacgtgtt cccgttcgcg cccgagcagc cggtcaccat
     4321 caacggcggc atgcgcatgc ccgcctggta cgacatcctc gggctggacc tggtgcggcg
     4381 cgaggacgag aaatcgctgc gcgcgtccgc cgcggccctg caggcgctcg tcgaccgcga
     4441 gatcgcgggc ggcatccccg cgtcgcgcat cgtgctggcc ggcttctcgc aaggctgcgc
     4501 gatgacgctg ctgaccggcc tgcgcgcgcc gcagcgcctg gcgggcctgc tgggcatgtc
     4561 gggctacctg ccgctggccg ccacgacggc cgccgagcgc agccaggcca accaggacgt
     4621 gccgatcttc ctgggccacg gccgcatgga tccggtggtg ccgttccagc gcggcatcga
     4681 cacgcgcgac gccctggtcg ccctcggcca tgacgtgcag tggcgcgact atccgatgca
     4741 gcactcggtc tgcgagcagg agatcgagga cacgcaggcg tggctgctga aggtgctggg
     4801 ctgagccgat gacacgacag ctgctccgat ggcccgcgct gctcgtggcg gcatgggtgg
     4861 cgggctgcgc cacgactccg gtggacggcc cgaggccgca gggcttcgat cttctcaacg
     4921 aaggcgccgg cgacgtgcac gacgtgcagg tccgctacgg cacgcatgtc ttcccgccgg
     4981 ccggcgccac tgccgtcgtg cgcgctccca cggtcacggg acagggcttc catcaggagg
     5041 ccaccgtggc gattcccgcg acggcacagg tccactgggt gtccgtcgac ggccggggac
     5101 acgacgccac ggtgccgatc gacaccctga tcgcggaccg ggccctgttc cacggcgtcc
     5161 agttcgtctt cgtcgatgac cacctggacg tggtgttcgc ctatgaacgg gaagcgccca
     5221 agccgcgcgg cctggactac ctgcgcacct ggtcgtcggc ggcgccgcgg ctgctctcgg
     5281 acccctgacg gcgccaggtc aggacggcgg cgcctgcgcg gcctcgccgg gctcgacgac
     5341 caccgcatcg cccaccgcca gcaggccgcc gcggatcacc cgcgcggtcg tgccgccgtg
     5401 gccgcgcatc gcgttgtagc ccccgggccc gagcagcgcc tccatcttcg agcatgggtc
     5461 gcacgggccc gtgacgacga tgacgacgtc cgcgccgatg cgcacctgca gcggccggtc
     5521 accgaacagc gcgtgctggg ccagcaggtt gacgcccgag atgacaaggt tgcgccgcag
     5581 gtcggcgggc gcgacgtcca tgcccgtcca ggcgcggatc aacggcaggt gttcggcctg
     5641 gatcagcgtg acctgccgct tgccgccgcc ggggcggctg gccggcttgt cgccgcggtg
     5701 gtcgccctcg aggccgcgct cggccagggc gcgggcggac ggcaccggca cggccggcgc
     5761 gtcgcggccg ggccgcagga cgatggcctc cagccggccg gcgcgcggga agcgcgcggt
     5821 cagcgagcgc aggtcgtcca tcgaagcagg gggacgggtc gcgcccgcgc gggggcggac
     5881 gcgcgagccg cgatcagagc ggcttcttgg ccagtttgcc cttgggggcg accgccgtca
     5941 ccggcgccgt cgggcgatcg gagctgacgg ccttcggcgg cgacgtgtcc ttcttcggct
     6001 tcttgacgtt cttggtggcg cgttgttcct tgcccatgag tcctccggcg ggttggtggt
     6061 gaattgggcg agatggtaag gcatcgcggc gctcgcctcg cgcgcgaagc cgcgcttgca
     6121 agcttgcgta ccgccccggc ccggggtctt tgccgccatc tgagaaaatc gacccatgcc
     6181 caggaccccg atgttcgccc cgctgcccga cggcccgctg gacgtggtcg gcgacgtcca
     6241 tggcgagctc gaggcgctgc tcgcgctgct gggccacctc ggctacgacg ccgacggcgg
     6301 ccacccggac gggcgccacc tcgtcttcgt cggcgacctg tgcgaccgcg ggcccgacag
     6361 ccccggcgtc gtcgacctcg tgcagcggct cgtggcttcg ggccgcgcgc tcgccacgct
     6421 gggcaaccac gagatcaacc tgctgcgcgg cgaccgcaag gacggcaacg actggttctg
     6481 gagcgagttc ggcgcgcgcg acgccaagta cgagccgcgc gcgcacctgc ccgccgacca
     6541 gcgcgacgac gtcatcgcgt tcctgcgcag gctgcccctg gccctggagc gcagcgacct
     6601 gcgcgtcgtg cacgccgcgt gggaccgcgc atcgatcgcg cagctgcgcg cggccgatcc
     6661 cgcgctgagc cgcgtcgagc acttcaggcg ctgggaggcc gaggccgacg cgcagatcgc
     6721 cgagcgcggc atcggcgagg cggcgcacgt cgagtatgcc gagtaccgcg agcgcctcgt
     6781 cgatcccggc caggccgtgc cgatgctgat ggccacggcg caggtcgacg agctgcgcca
     6841 gatgggcaac ccgatccgcg tggtcacgtc cggcatcgag cgcgtcgcga agaggccgtt
     6901 cttcgcgagc ggccactggc gcttcgtcga acgcatccgc tggtgggacg agtacggcga
     6961 ggacgtgccc gtcgtggtgg gccactactg gcgccagttc gcgcacgtcg atcgcgcgtc
     7021 gctcggcaag accgaggaga acctgttcac cgacctcgcg cccaccgcct ggcacggcct
     7081 gcgccgcaac gtgttctgca tcgacttctc ggtgggtggt cgattcctgg aacgaccgct
     7141 cccgcccggc gtgctcccgc gctcgcgcct ggccgcgctg cgctggcccg agcgcgtgct
     7201 ggcgctcgac acgggcgaga tgctgcccac gatcggggcc tgatcggggc gcatccgggc
     7261 tgcttcgtgg tcatcctgcg actgcgcttg gcttcgcgca ggctgacggc cgggtggggg
     7321 cttcgcgggc gtcgatcgag acatcggcgt ccagcgggaa tcgaggtcgg cggccatgcc
     7381 ttcattccgc gcgcagcgcc gccaggctgg cgcgcaggcg cggcgccgcg aagtccagga
     7441 aggtccgcaa cttggacgtc acgcgttgct ggccgtccca gacgaggtgc accggcacgg
     7501 gctcgggctc gaacggacgc agcagcaggc gcaagcgctt gtcgcgcagc gccgcgtcgg
     7561 cctggtacga cagcaggcgt gtcacgccca gccccgcgac cgccgcgtcg accgccgcct
     7621 cggccgtgtt gacgaccagc cgcggccgcg cgggcgtgaa gctcgcgccg ccggcgaagc
     7681 gccacggcgc gggaaacgtc gactcgaacg tgatgcagcg gtgcgcgtcg aggtcgtcgg
     7741 gcgtcctggg cgtgccgtgc gccttcagcc acgccgggct cgcgcagacg accgagcgca
     7801 actggcccag ccgcgtggcg ttgaggcggc tgtcgggcag cgcgccgatg cgcacggcga
     7861 cgtcgacatg ctcgtcgatc aggtgcgcga tgcggtcgcc gagcgccagc cgcacgtcga
     7921 cctcggcgtt ggcgcgcagg aactcgctga ccaccggcag cacgtgcagc cgcccgaaca
     7981 cgagcggcgc ggtgacggcc agctcgccgc gtggcgtgtc gtactcgccg gccgccgcgg
     8041 cctcggcgcc ggccacgtcg tcgaggatgc gccggcaggc ctgctcgtag ccccggccgg
     8101 gctccgtgag ctcgagccgg cgcgtggagc ggttgatcag gcgcgcgccc aggtgttcct
     8161 cgagttcggc cagcttgcgg ctgaccgtgg ccagcggcat gcccagccgc cgtcccgcgg
     8221 ccgacaggct gcccgcgtcc accacggcca ggaacacctt catcgcgtcg agccggtcca
     8281 tcgatactcc cgaatattgg aagagtgatt ccacattctg cggtctggtg aatccgattg
     8341 gaaggatcca ccatgagggc ctgggcgatc cgcccgcacc gaggacgagg aaatgaccag
     8401 caccgcctac cccagcgaca tcgccttcac gcccgccgtc aaggcgctgc agcaggcgcg
     8461 cggctcgcgc cgctcctatt cgcgcatgga gcagggccgt ggctggcaga cggcgatcga
     8521 cgacggcgtc cgcggcttca tcgaggcgca gacgacggtg ttcatggcca ccgccaacgc
     8581 cgtgggccag ccctacatcc agcaccgcgg cggcccggcc ggcttcctgc acgtgctgga
     8641 cgagcacacg atcgcgttcg ccgacttcag cggcaaccgc cagttcatca ccaccggcaa
     8701 cctggccgac aacccgaagg cgcagctttt cctgatggac tacgcgcacc ggcagcggct
     8761 gaagatctgg ggcgaggcgc gcgtcgagac ggatccggcc ctcgtcgagc gcctgatgcc
     8821 gccgggctac aaggcgcgcg ccgagcaggc gatcgtcttc acggtgaagg cctgggacgc
     8881 caactgcccg cagcacatcc cgcaactggt gccggtcgac gaggtgatcc cggcgatcgc
     8941 cgagcgcgat cgccgcatcg ccgagctgga aagcgaagtc gccgcgttga agaagtcggg
     9001 ctgatgtcac gcggccggcg tatcgtggcg ggatgagcca ccgcgccacc gtcggtcacc
     9061 aggtcttcgg cttcgccgat ctgcgcgagc tgatgggcaa ggccagcccg gcgcgctcgg
     9121 gcgacgcgct ggcgggcgtg gcggcggcga cggcgcagga acgtgtcgcg gcccaggccg
     9181 cgctggcgga cgtgccgttg aaggccttcc tgtcggaggc cgtggtgccg tacgagcacg
     9241 acgaggtcac gcgcctgatc gt
//
LOCUS       DATCRO010000642         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1019443, whole genome shotgun sequence.
ACCESSION   DATCRO010000642 DATCRO010000000
VERSION     DATCRO010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019443"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VIN75_17580"
     CDS             complement(<1..644)
                     /locus_tag="VIN75_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8976032.1"
                     /translation="MDIFLQQMINGLVLGSMYALVALGYTMVYGIVNLINFAHGEVLM
                     VGALTSWSVTVWLGPYNLPGWLLLVIALLCAVVVCSILNFTIEKVAYRPLRNAPRLAP
                     LITAMGMSLLLQTLAMIIWKPNYKSYPTLLPRDPIFIGGAVTNFTQITILVVTAVTLG
                     GLMWLVNRTRLGRAMRATAENPRVAALMGVRPDNVISATFVIGAALAALAGVMWA"
     gene            complement(1137..>2428)
                     /locus_tag="VIN75_17585"
     CDS             complement(1137..>2428)
                     /locus_tag="VIN75_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY8976033.1"
                     /translation="TKPVPPLAELMRPQALADVVGQQHLLGPGKPLRLAFESRKLHSF
                     ILWGPPGVGKTTLGRLAAKVTNSHFIAISAVLAGIKDIRAAIDEAQDQLDREGRQTVV
                     FVDEIHSFNKTQQDALLPHVESGLLTLVGGTTEHPGMQVTNALLSRAQVYNLQPLQQA
                     DFNELYERARPHLPAVTVTPDALALLGGYADGDGRRFLNLLEQVANAAGGAGLATVDD
                     TFAKSATSPSLRRADKSGDSYYEQISAFHKSVRGSQPDAALYWLARMIDGGVDPRQVT
                     RRMIAIASEDIGNADPRALQLAMNAAEAVDRLGLPECELALAQATTYLAAAPKSNASY
                     LAWKAAKEFVQRDGTRAVPIHLRNAPSKLMKQMGYGAQYRYAHDEPNGYAAGAQWFPD
                     DVPAQRWYQPVPRGMEIKIGEKLAALRALDDEHGDPA"
BASE COUNT          363 a          835 c          858 g          372 t
ORIGIN      
        1 gcccacatca cgccggccag cgcggccagc gcggcgccga tgacgaacgt cgcggagatg
       61 acgttgtcgg gacgcacgcc catcagcgcg gccacgcgcg ggttctcggc cgtggcgcgc
      121 atcgcgcggc ccaggcgggt ccggttgacg agccacatca ggcccccgag ggtcacggcc
      181 gtcacgacga ggatcgtgat ctgcgtgaag ttggtgacgg cgccgccgat gaagatcggg
      241 tcgcgcggca gcagcgtcgg gtacgacttg tagttcggct tccagatgat catggccagc
      301 gtctgcagca gcaggctcat gcccatcgcg gtgatcagcg gcgccagccg cggcgcgttg
      361 cgcagcgggc ggtaggccac cttctcgatg gtgaagttca ggatcgagca gactaccacc
      421 gcgcacagca gcgcgatcac cagcagcagc catccgggca ggttgtacgg cccgagccag
      481 acggtcaccg accaactggt cagcgccccc accatcaaca cttcgccgtg cgcgaagttg
      541 atcaggttga cgatgccgta aaccatcgtg tagcccagtg ccacgagcgc atacatgctc
      601 ccgagcacca ggccgttgat catctgctgc agaaagatat ccatcagggg gtctcctggt
      661 tctcaccgcc gcgacgccgc tccaacggcg cgcggcatcc tcgtgccttc agcatcgact
      721 tgccgcaggc atgccgccac gggcaatacc cgcatgcagc acgcaccggc cttgcaagaa
      781 cccggccacc cgcgacgccc gtggcgcgcc gatgacagag agcccgcaag ccctttgcgc
      841 tgtgcgggat tctaggagcg ccccgcagcg agagcggacg gacttacccg cgagaaaccg
      901 aacgacgcac cattgagaac cgcaaagacg gagcattgtt cgttgcggcg cgtgattcgc
      961 atgattcatg agatcggtgc gcgaccgccg cccgggcgcg ggcgtgacgc gcccgcgcgc
     1021 aggcacgggg cgcccatgtc gatactctct tgcgcaacgc cgatacgccg cggcgcgcgc
     1081 gccctgcgac ggtgcggacg ggcgcgcgcg gtgcccagcg acgccgcggg cgcggttcag
     1141 gccgggtcgc cgtgctcgtc gtcgagcgcc cgcagcgcgg cgagcttctc gccgatcttg
     1201 atctccatgc cgcgcggcac gggctggtac cagcgctggg ccggcacgtc gtcggggaac
     1261 cattgcgcgc cggcggcata gccgttgggc tcgtcgtgcg cataccgata ctgggcaccg
     1321 tagcccatct gcttcatcag cttgctgggc gcgttgcgca ggtggatcgg caccgcgcgg
     1381 gtgccgtcgc gctggacgaa ttccttggcc gccttccacg cgaggtagga cgcgttggac
     1441 ttgggcgccg ccgccaggta cgtggtggcc tgcgccagcg caagctcgca ttcaggcagg
     1501 cccagccggt cgacggcctc ggccgcgttc atggccagct gcagcgcgcg cgggtcggcg
     1561 ttgccgatgt cctcgctggc gatggcgatc atgcgccgcg tgacctggcg cgggtcgacg
     1621 ccgccgtcga tcatgcgcgc cagccagtaa agcgcggcgt cgggttgcga gccgcgcacc
     1681 gacttgtgga acgccgagat ctgctcgtag taggagtcgc cgctcttgtc ggcgcgccgc
     1741 agcgacgggc tggtggccga cttggcgaag gtgtcgtcca ccgtcgcgag gcccgcgccg
     1801 ccggccgcgt tggccacctg ttcgagcagg ttcaggaagc gccgcccgtc gccgtcggcg
     1861 tagccgccca gcagcgcgag cgcgtccggc gtcaccgtca cggcgggcag gtgcggccgc
     1921 gcgcgctcgt agagctcgtt gaagtcggcc tgctgcagcg gctgcaggtt gtagacctgg
     1981 gcacgcgaca gcagcgcgtt ggtcacctgc atgcccggat gctcggtggt gccgccgacc
     2041 agcgtcagca gtcccgactc gacgtgcggc agcagcgcgt cctgctgcgt cttgttgaac
     2101 gagtggatct cgtcgacgaa gaccacggtc tgccggcctt cgcgatcgag ctggtcctgc
     2161 gcctcgtcga tcgcggcgcg gatgtccttg atgccggcca gcacggccga gatcgcgatg
     2221 aagtggctgt tggtgacctt ggccgccagc cggcccagcg tcgtcttgcc cacgcccggc
     2281 gggccccaca ggatgaacga gtgcagcttg cgcgattcga aggccagccg cagtggtttg
     2341 ccggggccca gcaggtgctg ctggccgacg acgtcggcga gcgcctgcgg ccgcatcagc
     2401 tcggccaggg gcgggacggg tttcgtgg
//
LOCUS       DATCRO010000643        10694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_468509, whole genome shotgun sequence.
ACCESSION   DATCRO010000643 DATCRO010000000
VERSION     DATCRO010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468509"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VIN75_17590"
     CDS             <1..695
                     /locus_tag="VIN75_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8976034.1"
                     /translation="LAFTGDCLLIRGAGRCDFQQGHAGVLYRSITEQIFSLPDDCLVF
                     PAHDYSGRTMSCVGEERRHNPRIGGHADERDFVGFMENLHLPHPKQIAVALPANMRSG
                     QPEDGQVPRLADWAPVRQTYAGLIEIEPDWVSEHLSDVLLLDVRQHGEVDERLGRIAA
                     ARLLPLDELKGQLADLPRDRPVVTVCHAGMRSGQATVLLRQGGFTRVANMRGGMLLWH
                     QLGLPVVHGAAA"
     gene            902..1645
                     /locus_tag="VIN75_17595"
     CDS             902..1645
                     /locus_tag="VIN75_17595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8976035.1"
                     /translation="MTRPGFALRGALMLACAVAVAPAGAQVAPSAAAASAAERAQKEA
                     DRTMYWIRVLATKPAPVKPAAAPKPVAAAAPAAKPVADAREKVKVAAAATGAPGPITD
                     APGAANAAPSRVALAQAPTLSQPPIAGASDTSALSSNHADDVASGVADNPAPDAAPPV
                     APDPGLSQIKLVQPDFPPGVVKRVRKGNVEVRFEVDPDGTVSDAVVVQTSNPHLNEAA
                     IDAIRQWRFKPTPMSHTAAVNLVFDIDKE"
     gene            complement(1680..4223)
                     /locus_tag="VIN75_17600"
     CDS             complement(1680..4223)
                     /locus_tag="VIN75_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8976036.1"
                     /translation="MRVLYVEDNEQDADLTCRRLARLAPDVAVSTVHTLADARAHLAA
                     AEASGALPDVLLVDVRLPDGNGLELLGEVRARGLPVAVVMLTGSGDEALVVAALRGGA
                     DDYVVKAGGYIDRLPATFAGVVDAFRAATVQRSTPIRVLYAEPSADDVDLLQRHLARY
                     APHLQVQAVGTAQEAFARLPPAVPDDAAPDVLLLDFKLPGVTALDVVREVRHERGLRL
                     PVIVITGHGDETSAAQTLKLGATDYLVKQEDLLNRLPVAIENAWYRGQLERERGALRE
                     SEERFRQMAESIGDVFWLSDPQRRLALYVSPAFERIWHMPVSEIYADWRAWLRHVHED
                     DREPLRVRMESLAPDEEVELEYRVCPPGRETRHVVMRVYVVRDGEGRDVRRAGVVQDV
                     TERKAQEARIEHLAYHDGLTGLPNRTMLMDRLGQALSQAQRLEQQVAVLFLDLDRFKP
                     INDSLGHLVGDQLLREIARRLRAALRENDTVARVGGDEFQVVVCNVAGATGAARIAEK
                     LMRALGEPFTIEGQELHVTASLGVSLFPRDGASGELLLKFADIALYEAKGEGRNAYRF
                     FSPEMNAQAHGRLRLENDLRRAVERGELELHYQPQLDLAIGEVCAVEALVRWRHPERG
                     LVLPNQFIPMAEETGLVLGIGEWVLGEACRQVAQWQREGLVRGSAPLRVAVNISARQL
                     QRPGLDGAVRRALADSGLPAACLELEITESSVMLDPQHAQSVLQSLRELGVQLSIDDF
                     GTGYSSLAYLKRLPLDRLKIDRSFIGGIPTDSDDAAIVETIIVMTHKLGLRVIAEGVE
                     TLEQRLQLVRQGCDEMQGFLLAHPVPAGELPALLERLQTAASGAGLGTS"
     gene            complement(4234..4656)
                     /locus_tag="VIN75_17605"
     CDS             complement(4234..4656)
                     /locus_tag="VIN75_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976037.1"
                     /translation="MEDRRPILLVEDNPMDVDLTQRAFAKRRIVNTLQVARDGEEALA
                     WLARWDAGEPAPVVILLDLKLPRVSGLEVLRQLKAHADWSRIPVVVLTTSREDADVAA
                     AYALGVNSYIVKPVEFEKFIDVAAQIDLYWCVLNQQAL"
     gene            complement(4739..6673)
                     /locus_tag="VIN75_17610"
     CDS             complement(4739..6673)
                     /locus_tag="VIN75_17610"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8976038.1"
                     /translation="METEINRRILDSLESGLMWFDAERRIVDANRAAREVLGLEERHF
                     GARIEDLGWAQSDEHGMPMPEEARPLRRALDGSGETTSGIVVVDTSARGRRWLRIRAT
                     PVDCIAAGRGRGAIVSFTDFTEERAAARALMETEQLLRMFGDHSMDLLWIVDPGTRRL
                     LYMNSAYERIWGRPAAPLYEDLSHWMDGIHPDDRERVAEAARHADPSGMYQTEYRVIR
                     PDGRLVWIRGRGFPLFAPDGRMRYLAGFAEDITLARVNEELERAHRVAQERLERIVAT
                     APGAVHSYCRDAQGQGRFVFASPAIRDLLGVDATTLVARRIDLLELVHPDDRALVEQS
                     LRESADLGSAWLAIFRVPLVRGGERCVEAHSMPVREDDGGVVWHGFLIDITDRRRAEQ
                     EIRSLNVELENRVAERTSELEAKHREMESFTYSVSHDLKAPLRGIDGYSRLLESDHGD
                     QLDDEGRFFVATIRKATAHMGELIDDLLAYSRIERGRPRLGPVESGPVVASVVDGAAG
                     AIADGGIALRSRVDPGVTALGEHDGLVLALRNLLDNAIKFTAGRPDRIIEIGARRAQG
                     HALFWVRDNGPGFDMRYHDRIFEIFQRLHRAEDYPGTGVGLAIVRKAVERMHGKVWAE
                     SAKGAGATFWIQLPEAGTPA"
     gene            complement(6705..8354)
                     /locus_tag="VIN75_17615"
     CDS             complement(6705..8354)
                     /locus_tag="VIN75_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976039.1"
                     /translation="MRALARLTEELESRPDDADRVDALARWLRAAGRDAGALALAWLV
                     AGSKAARPPRVTQAALLEAARALAAGQGTAPWLFDAGIAAADELAEAIALMLPWPDAA
                     ARPELADWLADWSAAGAQPTASRADAVARAIARVDDALARRWAVRAACGLARPTVDDW
                     QWQRAWARAFGLDAQAVAWWWHERREPLRHDLPPGALPRPHDAAPLSDAPATLHETLR
                     AAWQRGEWHAQPRWRGVRVHVVRRGERVAVWQVGGPLLNARLPADWLAPAPWPEHGVV
                     EALLLAWHAGRIAPPDEAWATRPRAGGPTLHLALADWHGEPASAAERRAQLRARWPEA
                     DPAQVAALPPIFMLPTLAPSGDTLQVRADAARAHGGCGLVLRAASSHVAWTVRAGLHR
                     VRAVLQYVPSELLVADPAAAGGLAGAGCGFALWNRMPRSEAEQHAAMTASLSGEFLPP
                     PDDAPGTEGLRLLPLARLPIELPDGELPALHAWLREHAGQRFGGVQAVAPVQVFEIGF
                     AQARPSRRHRIGATLAGARVLRWLRDAPPGGAQLADDVIPD"
     gene            complement(8422..9018)
                     /locus_tag="VIN75_17620"
     CDS             complement(8422..9018)
                     /locus_tag="VIN75_17620"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976040.1"
                     /translation="MNSLHTPHDRRAPGAAHPRLVIVPGLHGSGAEHWQSWLHGQVAG
                     AVRVEQEAWSTPDLERWSDRVADTVAALGAGPHVVVAHSFGCLATVRALARRPSLHIA
                     QLLLVAPAEPNRFDVAAALPSTRLAVRSCVVASDDDPWMSATQAHAWALRWGSDWINL
                     GNAGHINVESGYGPFPLAREWATGALNRLDTRPWRQAA"
     gene            9425..10246
                     /locus_tag="VIN75_17625"
     CDS             9425..10246
                     /locus_tag="VIN75_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976041.1"
                     /translation="MLTPSTPPVATSFSYDSTPTAKAAAVEANARADFRQQVQAETRN
                     DAHLADIAATQRAARDAASTIPDPMTSLSTVAVAGAMSSVPAALEMMRSIALQSAAGS
                     LSDTDRQTLHAEYAQLTAHVATTVGSVGASEPQTPARDDEREDNAGNTWRESSDDRRG
                     TLAQTRVVAQQTVQAEPVQHDVTVQHQVLVADGHGPPPAPRVDLRTHDLHVGADSYAP
                     VSVRQAVTLPVEPASVARLETRAETQRTTATQAAQITQFVQVSQTRQVPPVVGVA"
     gene            complement(10287..>10694)
                     /locus_tag="VIN75_17630"
     CDS             complement(10287..>10694)
                     /locus_tag="VIN75_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976042.1"
                     /translation="PRPSHGARDLTRILVVDDLKDITETMGVLFEALGHDTKVAADGR
                     QAVRTTLEFEPDIIFLDLDMPVLNGYEAAREIRGAPLPRQPFLIALTKAHGTTVEVAT
                     RAVGFDFYLRKPVDTNALLALVADLSTRTRQAG"
BASE COUNT         1473 a         3993 c         3878 g         1350 t
ORIGIN      
        1 gcctggcctt caccggcgac tgcctgctga tccgcggcgc aggccgctgc gacttccagc
       61 agggccacgc cggcgtgctg taccgatcga tcacggagca gatcttcagc ctgcccgacg
      121 actgcctcgt ctttccagcg cacgactaca gcggccgcac gatgtcgtgc gtcggcgagg
      181 agcgccggca caacccgcgc atcggcgggc acgccgacga gcgcgacttc gtcggcttca
      241 tggagaacct gcacctgccc catcccaagc agatcgcggt ggcgctgccg gccaacatgc
      301 gctccgggca gcccgaggac ggccaggtgc cgcgcctggc cgactgggcg ccggtgcgcc
      361 agacctacgc cggcctgatc gagatcgagc ccgactgggt ctccgagcac ctatccgacg
      421 tgctgctgct ggatgtgcgc cagcacggcg aggtcgacga gcgcctgggc cgcatcgccg
      481 cggcacggct gctgccgctc gacgagctca aggggcagct ggccgacctg ccgcgcgacc
      541 gccccgtcgt caccgtctgc catgccggca tgcggtccgg ccaggccacc gtgctgctcc
      601 ggcagggcgg cttcacgcgc gtggccaaca tgcgcggcgg catgctgctg tggcaccagc
      661 tgggcctgcc cgtcgtgcac ggcgcggcag cctgaacaac ggcgcccgcc cgccagcggg
      721 gcggcgcgcg gtccgcgcga aagcgtgacg aagtgcgctc gctcgcccga acgcgcggcg
      781 cgaaggccgc cttggcggcg cctcgcgtcc tacactgcgg cacgcctgcg cgaccctgtg
      841 cgcaggcagg cgggtgccgc acgagacgac ggtgccctgg agtcaaacga gcaggattgc
      901 catgacccga cccggatttg cgctgagggg cgccttgatg ctggcctgcg ccgtggcggt
      961 cgcgccggcg ggcgcgcagg tcgcgccgtc ggcggcggcc gcgtccgccg cggagcgcgc
     1021 gcagaaggaa gccgaccgca ccatgtactg gatccgcgtg ctggccacca agccggcgcc
     1081 ggtcaagccg gccgccgcgc ccaagcccgt ggcggcggcc gcgcccgcgg ccaagccggt
     1141 cgcggacgcc cgcgagaagg tcaaggtcgc cgcggccgcg acgggcgcgc ccgggccgat
     1201 caccgacgcg ccaggcgccg ccaacgccgc cccgtcccgc gtcgcgctgg cccaggcgcc
     1261 gacgctctcg cagccgccga tcgccggcgc ctccgacacc tccgcgctga gcagcaacca
     1321 cgccgacgac gtcgcctccg gcgtggccga caacccggcg ccggacgccg cgccgccggt
     1381 cgcgcccgat cccgggctga gccagatcaa gctggtgcag cccgacttcc cgccgggcgt
     1441 ggtcaagcgc gtccgcaagg gcaacgtcga agtgcgcttc gaggtcgatc cggacgggac
     1501 ggtgtcggac gccgtcgtcg tccagacctc gaatccgcac ctgaacgaag cggccatcga
     1561 cgcgatcagg cagtggcgct tcaagcccac gccgatgtcg cacaccgcgg cggtgaacct
     1621 ggtcttcgac atcgacaagg aatagcgggc ggcacctcgt cccgcgccgg gaacggggcc
     1681 taggacgtgc ccaggcccgc gccgctggcc gccgtctgca gccgctccag cagcgccggc
     1741 agctcgccgg ccggcaccgg gtgcgcgagc aggaagccct gcatctcgtc gcagccctgg
     1801 cgcaccagtt gcaggcgctg ttccagcgtc tcgacgccct cggcgatcac gcgcaggccc
     1861 agcttgtgcg tcatcacgat gatggtctcg acgatggcgg catcgtcgga gtcggtcggg
     1921 atgccgccga tgaacgagcg gtcgatcttg aggcggtcca gcggcagccg cttcaggtag
     1981 gcgaggctgg agtagccggt gccgaagtcg tcgatcgaca gctgcacgcc gagctcgcgc
     2041 agcgactgca gcacgctctg cgcatgctgc gggtcgagca tcacgctgct ctcggtgatc
     2101 tcgagttcga ggcacgcggc gggcaggccg ctgtcggcca gcgcgcgccg cacggcgcca
     2161 tcgaggccgg gccgctgcag ctggcgcgcc gaaatgttga cggccacgcg cagcggcgcg
     2221 ctgccgcgca cgaggccctc gcgctgccac tgcgccacct ggcggcaggc ctcgcccagc
     2281 acccattcgc cgatgcccag caccaggccc gtctcctcgg ccatcgggat gaactggttc
     2341 ggcagcacga ggccgcgttc cggatggcgc cagcgcacca gcgcctccac cgcgcagacc
     2401 tcgccgatcg ccaggtccag ctgcggctgg tagtgcagct ccagctcgcc ccgctcgacc
     2461 gcgcggcgca ggtcgttctc cagccgcagc cggccgtgcg cctgggcgtt catctcgggg
     2521 ctgaagaatc ggtaggcgtt gcggccctcg cccttggcct cgtacagcgc gatgtcggcg
     2581 aacttgagca gcagctcgcc gctggcgccg tcgcgtggga acaggctcac gcccaggctc
     2641 gcggtgacgt gcagctcctg gccctcgatc gtgaacggct cgccgagcgc gcgcatcagc
     2701 ttctcggcga tgcgcgccgc gccggtcgcg ccggcgacgt tgcacaccac cacctggaac
     2761 tcgtcgccgc cgacacgggc cacggtgtcg ttctcgcgca gcgcggcgcg caggcggcgc
     2821 gcgatctcgc gcagcagctg gtcgcccacc aggtggccca gcgagtcgtt gatcggcttg
     2881 aagcggtcga ggtcgaggaa cagcacggcc acctgctgct ccagccgctg cgcctgcgac
     2941 agcgcctggc cgagccggtc catcagcatc gtgcgattgg gcaggccggt gaggccgtcg
     3001 tggtaggcga ggtgctcgat gcgcgcctcc tgcgccttgc gctcggtcac gtcctgcacg
     3061 acgccggcgc ggcgcacgtc gcggccctcg ccgtcgcgca cgacgtacac gcgcatgacc
     3121 acgtgccgcg tctcgcggcc cggcgggcag acgcggtact cgagctccac ctcctcgtcc
     3181 ggggcgaggc tttccatgcg cacgcgcagc ggctcgcggt cgtcctcgtg cacgtggcgc
     3241 agccaggcgc gccagtcggc gtagatctcg ctgaccggca tgtgccagat gcgctcgaac
     3301 gccggactca cgtacagcgc aagccggcgc tgcgggtcgc tgagccagaa cacgtcgccg
     3361 atcgattcgg ccatctggcg gaagcgctcc tcgctctcgc gcagcgcgcc gcgttcgcgc
     3421 tccagctgcc cgcggtacca ggcgttctcg atggccaccg gcaggcggtt cagcagatcc
     3481 tcctgcttga cgaggtagtc ggtggcgccc agcttgagag tctgcgcggc gctcgtctcg
     3541 tcgccatggc ccgtgatgac gatgacgggc aggcgcaggc cgcgctcgtg gcgcacctcg
     3601 cgcacgacgt ccagcgccgt cacgccgggc agcttgaaat cgagcagcag gacgtcaggc
     3661 gccgcgtcgt ccggcacggc cggcggcagg cgcgcgaacg cctcctgcgc ggtgcccacg
     3721 gcctgcacct gcaggtgggg agcgtagcgc gcgagatggc gctgcagcag gtcgacgtcg
     3781 tcggcgctcg gctcggcgta cagcacgcgg atgggcgtcg agcgctgcac cgtggcggcg
     3841 cggaacgcgt cgacgacgcc cgcaaacgtg gccgggagcc ggtcgatgta gccgcccgcc
     3901 ttgaccacgt agtcgtccgc gccgccgcgc agcgcggcca ccaccagcgc ctcgtcgccc
     3961 gagccggtga gcatgacgac cgccaccggc aggccgcgcg cgcgcacctc gcccagcagc
     4021 tccaggccat tgccgtcggg caggcgcacg tcgacgagca gcacgtcggg cagcgcgccg
     4081 gaggcctcgg ccgctgccag gtgcgcacgg gcgtcggcca gcgtgtgcac ggtgctcacg
     4141 gcgacgtcgg gcgcgagccg ggccaggcgc cggcaggtca ggtcggcgtc ctgttcgttg
     4201 tcttcgacgt acaggactct cacgcgaggc tttctagagc gcttgctggt tcagcacgca
     4261 ccagtacagg tcgatctgcg ccgccacgtc gatgaacttc tcgaactcca cgggcttgac
     4321 gatgtaggag ttgacgccca acgcgtacgc cgcggcgacg tccgcgtcct cgcgcgaggt
     4381 ggtgagcacc acgaccggga tgcggctcca gtcggcgtgc gccttgagct ggcgcagcac
     4441 ctccaggccg ctgacgcgcg gcagcttcag atcgagcagg atcaccaccg gcgcgggctc
     4501 gcccgcgtcc cagcgcgcca gccatgcgag cgcctcctcg ccatcgcgcg ccacctgcag
     4561 cgtgttgacg atgcgccgct tggcgaacgc acgctgcgtc aggtcgacat ccatcgggtt
     4621 gtcctcgacg agcaggatcg ggcgacgatc ttccatgcgg acagcatagc gaaacggcca
     4681 cgcttgtaca ccacgtttgc cccgccccgg gcccgcgcgg tcgagtgcgc atgccgcttc
     4741 aggcgggcgt cccggcctcc ggcagctgga tccagaacgt ggcgccggcg cccttggcgc
     4801 tctcggccca gaccttgccg tgcatgcgct ccaccgcctt gcgcacgatg gccaggccca
     4861 cgccggtgcc cgggtagtcc tcggcgcggt gcagtcgctg gaagatctcg aagatgcggt
     4921 cgtggtagcg catgtcgaac ccggggccgt tgtcgcgcac ccagaacagc gcatggccct
     4981 gggcgcggcg cgcgccgatc tcgatgatgc ggtcgggccg gccggcggtg aacttgatgg
     5041 cgttgtccag caggttgcgc agcgccagca cgaggccgtc gtgctcgccc agcgccgtca
     5101 cgccggggtc gacgcgcgag cgcagcgcga tgccgccgtc ggcgatcgcg ccggccgcgc
     5161 cgtcgacgac gctggccacg acgggccccg actccaccgg ccccagccgc ggccggccgc
     5221 gctcgatgcg cgagtaggcg agcaggtcgt cgatcagctc gcccatgtgc gccgtcgcct
     5281 tgcggatggt ggccacgaag aagcggccct cgtcgtcgag ctggtcgccg tggtcggact
     5341 ccagcagccg gctgtagccg tcgatgccgc gcagcggcgc cttcaggtcg tgcgacaccg
     5401 agtacgtgaa cgactccatc tcgcggtgct tggcctccag ctcggacgtg cgctcggcca
     5461 cgcggttctc cagctcgacg ttgagcgaac ggatctcctg ctcggcgcgc cgccggtcgg
     5521 tgatgtcgat caggaagccg tgccagacga cgccgccgtc gtcctcgcgc acgggcatcg
     5581 agtgcgcctc gacgcagcgc tcgccgccgc gcacgagcgg cacgcggaag atggccagcc
     5641 acgcgctgcc gagatcggcg gactcgcgca gcgactgctc gacgagcgcg cgatcgtccg
     5701 ggtgcacgag ctcgagcagg tcgatgcggc gcgccacgag cgtcgtcgca tcgacgccca
     5761 gcaggtcgcg gatggccggg ctggcgaaca cgaagcggcc ctggccctgc gcgtcgcggc
     5821 agtacgagtg caccgcgccc ggcgccgtcg cgacgatgcg ctccaggcgc tcctgcgcga
     5881 cgcggtgcgc gcgctcgagc tcctcgttga cacgcgccag cgtgatgtcc tcggcgaacc
     5941 cggccaggta gcgcatgcgg ccgtccggcg cgaacagcgg gaagccccgg ccgcggatcc
     6001 acacgagtcg gccgtcgggg cggatgacgc ggtactcggt ctggtacatg cccgaagggt
     6061 cggcatggcg cgccgcctcg gccacgcgct cgcgatcgtc cgggtggatg ccgtccatcc
     6121 agtgcgacag gtcctcgtac agcggcgccg ccggccggcc ccagatgcgc tcgtaggccg
     6181 agttcatgta cagcagcctg cgcgtgcccg ggtcgacgat ccacagcagg tccatcgagt
     6241 ggtcgccgaa catgcgcagc agctgctcgg tctccatcag cgcacgcgcg gcggcgcgct
     6301 cctcggtgaa gtcggtgaac gagacgatcg cgccgcgacc gcggccggcg gcgatgcagt
     6361 cgaccggcgt cgcgcggatg cgcagccagc gccggccgcg cgccgaggtg tcgacgacga
     6421 cgatgcccga cgtcgtctcg ccgctgccgt cgagcgcgcg ccgcagcgga cgcgcctcct
     6481 cgggcatggg catgccatgc tcgtcggact gggcccagcc gagatcctcg atgcgtgcgc
     6541 cgaaatgccg ctcctcgagc ccgagcacct cgcgcgccgc gcggttggca tcgacgatgc
     6601 ggcgctcggc atcgaaccac atcaggcccg actccagcga atcgaggatg cgccggttga
     6661 tctcagtctc cacgcgaact cctggtcacg tcgagtggtg gactttagtc ggggatgacg
     6721 tcgtccgcca actgggcgcc acccggcggc gcatcgcgca gccagcgcag cacgcgcgcg
     6781 ccggcgagcg tcgcaccgat gcggtggcgc cggctgggcc gggcctgcgc gaagccgatc
     6841 tcgaagacct gcaccggcgc caccgcctgg acgccgccga agcgctggcc cgcatgctcg
     6901 cgcagccagg cgtgcagggc cggcagctcg ccgtcgggca gctcgatggg caggcgtgcg
     6961 agcggcagca gccgcaagcc ctcggtgccg ggcgcgtcgt cgggcggcgg caggaattcg
     7021 ccggacagcg aggccgtcat ggctgcatgc tgctcggcct cggaacgcgg catgcggttc
     7081 cacagcgcga agccgcagcc cgcgccggcc agtccccccg cggccgcggg gtccgccacc
     7141 agcagctccg agggcacgta ctgcagcact gcgcggacgc gatgcaggcc ggcgcgcacc
     7201 gtccaggcga cgtgcgacga cgcagcgcgc aggacgaggc cgcaaccgcc gtgcgcgcga
     7261 gccgcgtcgg cgcggacctg cagggtgtcg ccggacggcg ccagcgtcgg cagcatgaag
     7321 atgggcggca acgcggcgac ctgcgccggg tcggcctcgg gccagcgcgc gcgcagttgc
     7381 gcccggcgtt cggcggccga cgccggctcg ccgtgccagt cggccagcgc caggtgcagc
     7441 gtcgggccgc cggcgcgcgg gcgcgtggcc caggcttcgt ccggcggcgc gatgcggccc
     7501 gcgtgccagg ccagcagcag cgcctcgacg acgccgtgct cgggccacgg cgcgggggcg
     7561 agccagtccg ccggcagtcg agcgttcagc aatgggccac cgacctgcca gacggcgacg
     7621 cgctcgccgc gccgcacgac atgcacgcgc acgccccgcc aacgcggctg cgcgtgccac
     7681 tcgccgcgct gccaggcggc ccgaagggtc tcgtgcagcg tcgcgggcgc gtcggacagc
     7741 ggcgcggcgt cgtgcggccg tgggagggcg ccgggcggca ggtcatgtcg cagcggctcg
     7801 cggcgctcgt gccaccacca ggcgacggcc tgcgcgtcga gcccgaacgc gcgggcccag
     7861 gctcgctgcc actgccagtc gtcgaccgtc ggacgggcca gcccgcaggc ggcgcgcacg
     7921 gcccagcggc gcgccagggc gtcgtcgacg cgggcgatgg cgcgggcgac cgcgtccgcg
     7981 cgcgacgcgg ttggctgggc gccggcggcg gaccagtcgg ccagccagtc cgccagctcc
     8041 gggcgcgcgg ccgcgtccgg ccacggcagc atcagcgcga tcgcctcggc cagttcgtcg
     8101 gccgcggcga tgccggcgtc gaacagccag ggcgcggtgc cttggccggc agcgagcgcg
     8161 cgcgcggcct cgagcaacgc ggcctgcgtc acgcgcggcg ggcgcgcggc cttcgacccc
     8221 gcgaccagcc acgcgagcgc gagtgcgccc gcatcacgcc cggccgcgcg cagccagcgc
     8281 gcgagcgcgt caacgcgatc ggcgtcgtcc gggcgcgatt ccagttcttc ggtgaggcgg
     8341 gccagggcgc gcatcccggc atgatgccgt gcgcgcggcg ggtgcgcgcc caggcctggc
     8401 ccgggcctgg cccgggcgcc ctcaggcggc ttggcgccag ggtcgcgtgt cgagccggtt
     8461 cagcgcgccg gtggcccact cgcgcgccag cgggaacggc ccgtagccgg actcgacgtt
     8521 gatgtggccg gcgttgccga ggttgatcca gtcgctgccc cagcgcagcg cccaggcgtg
     8581 cgcctgggtg gcggacatcc acgggtcgtc gtcgctcgcg accacgcagc tgcgcacggc
     8641 caggcgcgtc gacggcagcg ccgcggcgac gtcgaaccgg ttcggttcgg ccggcgccac
     8701 cagcagcagc tgggcgatgt gcagcgacgg gcgcctggcg agcgcccgca ccgtcgccag
     8761 gcagccgaag ctgtgcgcga cgaccacgtg cgggccggcg ccgagcgcgg cgacggtgtc
     8821 ggccacccgg tcgctccagc gctccaggtc gggcgtgctc caggcctcct gctcgacgcg
     8881 caccgccccc gccacctgtc cgtgcagcca gctctgccag tgctcggccc cgctgccatg
     8941 cagtccgggc acgatcacga gacgcggatg ggccgcgccg ggcgcgcggc ggtcgtgggg
     9001 cgtgtggagc gagttcatgg ctgccagcgt aggggcggcg gccggccggg ccaacgaagg
     9061 aaatgcgata tgcaagcacg cgccgggagg ggaccggcct ctcctcgaat cgcgccggca
     9121 agccgatcgc agccgtggcg ttggacgcga tccgaggcga aggccagacc cctccggcct
     9181 cgccaaaaaa tcttgaaaaa agccctcaag ttcccctgga ccccgccgaa tacccgtcca
     9241 agcaccgtcc tcgactagca ccaacgccaa ccggagcatc gtcggaacag cccaggaagg
     9301 tgccggcatt tcttcacgtc cccgtccgcg cggctgtgcc aggcacttcc gcgccggtgg
     9361 tggcgtgggc aagatatcga gcgcgtgccg cctgctttcg ggtcgcacgc cgaaggacct
     9421 cgtcatgttg acgcccagca ccccgcccgt cgccacgtct ttctcctacg acagcacgcc
     9481 caccgcgaag gcggcggctg tcgaggcgaa cgcgcgcgcc gatttccggc agcaggtgca
     9541 ggcggagacg cgcaacgacg cgcacctggc cgacatcgcc gccacgcagc gcgccgcgcg
     9601 cgacgcggcc agcacgattc ccgaccccat gaccagcctg tcgaccgtcg cggtcgcggg
     9661 tgccatgagc agcgtgccgg ccgcgctcga gatgatgcgc agcatcgcgc tgcagtcggc
     9721 cgccgggtcg ctgagcgaca ccgaccgcca gacgctgcac gccgagtacg cgcagctcac
     9781 ggcgcacgtg gccaccacgg tcggcagcgt cggcgcgagc gagccgcaga ccccggcgcg
     9841 cgacgacgag cgcgaggaca acgccgggaa cacctggcgc gagagcagcg acgatcgccg
     9901 gggcacgctc gcgcagaccc gggtcgtggc gcagcagacg gtgcaggccg agccggtcca
     9961 gcacgacgtc accgtgcagc accaggtgct ggtggccgac ggccacgggc cgccgccggc
    10021 cccgcgcgtc gacctgcgca cgcacgacct gcacgtgggc gccgattcct acgcgccggt
    10081 gtcggtgcgc caggcggtga cgctgccggt cgagcccgcc agcgtcgcgc gtctggagac
    10141 ccgcgccgag acgcagcgca cgacggccac gcaggccgcg cagatcacgc agttcgtgca
    10201 ggtttcgcag acccggcagg tcccgccggt cgtcggcgtc gcctgagcgc cggcgcggac
    10261 cgcgaacacg cgcggcccgc gccggatcat cccgcctggc gcgtgcgcgt cgacagatcg
    10321 gccaccaatg ccagcaaggc gttggtgtcg accggcttgc gcaggtagaa gtcgaagccg
    10381 accgcccggg tggccacttc gacggtggtg ccgtgggcct tcgtgagcgc gatcaggaac
    10441 ggctggcgcg gcagcggcgc accgcggatc tcgcgggcgg cttcgtagcc gttgagcacc
    10501 ggcatgtcca ggtccaggaa gatgatgtcc ggctcgaatt cgagcgtcgt gcgaacggcc
    10561 tgccgcccgt cggcggcgac cttggtgtcg tggccgagcg cctcgaagag cacacccatc
    10621 gtctcggtga tgtccttgag gtcgtcgact accaggatcc tggtcaagtc tctagctcca
    10681 tgagagggtc gcgg
//
LOCUS       DATCRO010000644         4943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_817166, whole genome shotgun sequence.
ACCESSION   DATCRO010000644 DATCRO010000000
VERSION     DATCRO010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817166"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="VIN75_17635"
     CDS             complement(<1..2288)
                     /locus_tag="VIN75_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY8976043.1"
                     /translation="MQGIASTINETGAAVHAAPAQTVVGSQSAYQGYQIIRRNGAVVS
                     FEPSKIAVALMKAFLAVHGTQGAASASVRETVDGLTESVVRALLRSRPAGGTFHIEDI
                     QDQVELGLMRGSHHEVARAYVLYRERRAQDRIKAGEVPTRAAEPELFVTDKGERVPLD
                     VAHLRSLIEAACANLGADVVAEPVFAETRRNLYDGVPMDEVFKAAILASRTLIEKEPA
                     YTKATARLLMHTIRKEILGEEVSQEQMGERYAEYFPRFIKRGIKAELLDEKLAQFDLA
                     RLGAALDASRDMQFDYLGLQTLFDRYFLHENDVRIELPQAFFMRVAMGLALGEIDREA
                     RAIEFYNVLSTFDFMSSTPTLFNSGTRRSQLSSCYLTTVADDLDGIYEALKENALLSK
                     FAGGLGNDWTNVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLE
                     TWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMRRVMEGGDWTLFSPSTCTDLH
                     DLVGLEFEKAYTAYEAAADRGELKLFKRVPAKDLWRRMLTMLFETGHPWITFKDACNV
                     RSPQQHVGVVHSSNLCTEITLNTSETEIAVCNLGSVNLAQHLVAGADGQLGIDHAKLQ
                     KTVRTAMRMLDNVIDINYYAVKKARDSNLRHRPVGLGIMGFQDCLYQLRTPYASEAAV
                     EFADRSMEAVCYYAYSASTELAAERGRYSSFKGSLWDKGILPFDSIDLLAKNRGGYVE
                     VDRSITLDWDALRAKIAQHGMR"
     gene            complement(2855..3436)
                     /gene="ampD"
                     /locus_tag="VIN75_17640"
     CDS             complement(2855..3436)
                     /gene="ampD"
                     /locus_tag="VIN75_17640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY8976044.1"
                     /translation="MHSDWDDGWWRHARRVPSPNFRARPGDARVDTVVLHSISLPPGE
                     YGGDGVERLFTNTLDWSAHPYFETIRGLEVSAHFLVRRDGSVLQFVSIDDRASHAGRS
                     SWAGRSEANDWSIGVELEGLEGDTFEPAQYDALARLLPAIDRVRGIRHVVGHEHVAPG
                     RKIDPGPGFDWPRLASQLHWDDERIPPAKPRGD"
     gene            complement(3495..>4943)
                     /locus_tag="VIN75_17645"
     CDS             complement(3495..>4943)
                     /locus_tag="VIN75_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8976045.1"
                     /translation="LTLLREGLEVETAATVAQAREILQTRLFRAVISDMRLPDGTGLD
                     LLQWLEQSGRPEKVIVVTAYGSPENAVTALKAGAFDYLTKPVDLRQFRAVVASAMGRS
                     RATPAPPAGDATPSDGKGDPLRRLVGSSDAMANVRGLVEKVARSMAPVLVQGESGTGK
                     ELVARAIHDVSARAGQPFVAVNCGAIPETLLEAEFFGVRKGAFTGASADRDGFFQAAN
                     GGTLFLDEIGDLPFAMQSKLLRAIQERAVRAIGATSEQTMNVRIVSATHKDLAAEVQA
                     GRFRQDLFYRLNVIDIKVPPLRERLVDLPAIADALLARLAREVGVAPARLSPDARALL
                     ARYAFPGNVRELENLLHRAVALSDGEMIVPADLALPEALLADTRPAPPPRDPDADVIA
                     AVVVDDHDGPPAAEAIGAAVPAAPAAGSEPVPADLVRYLDEVEREILVRALERQNFNR
                     TAAGASLGLSLRQMRYRMARLGITESEASDPT"
BASE COUNT          704 a         1668 c         1778 g          793 t
ORIGIN      
        1 ttgcgcatgc cgtgctgggc gatcttggcg cgcagcgcgt cccagtcgag cgtgatcgag
       61 cggtcgacct cgacgtagcc gccgcggttc ttggccagca ggtcgatcga gtcgaacggc
      121 aggatgccct tgtcccacag cgagcccttg aacgacgagt agcggccgcg ctcggcggcg
      181 agctcggtcg aggccgagta cgcgtagtag cagacggctt ccatcgagcg gtcggcgaat
      241 tcgaccgcgg cctcgctggc gtacggcgtg cgcagctggt acaggcagtc ctggaagccc
      301 atgatgccca ggcccaccgg gcgatggcgc aggttcgagt cgcgcgcctt cttgaccgcg
      361 tagtagttga tgtcaatgac gttgtcgagc atgcgcatcg cggtgcgcac cgtcttctgc
      421 agcttggcgt ggtcgatgcc cagctggccg tcggcaccgg cgaccaggtg ctgggccagg
      481 ttgaccgagc cgaggttgca gacggcgatc tcggtctcgc tcgtgttgag cgtgatctcg
      541 gtgcacaggt tggacgagtg cacgacgccg acgtgctgct gcggcgagcg cacgttgcag
      601 gcgtccttga acgtgatcca ggggtggccc gtctcgaaca gcatcgtcag catgcggcgc
      661 cacaggtcct tggccggcac gcgcttgaac agcttgagct cgccgcggtc ggcggcggct
      721 tcgtaggcgg tgtaggcctt ctcgaactcc aggcccacga ggtcgtgcag gtcggtgcag
      781 gtcgacggcg agaacagcgt ccagtcgccg ccttccatca cgcggcgcat gaacaggtcg
      841 ggaatccagt tcgccgtgtt catgtcgtgg gtgcggcggc ggtcgtcgcc ggtgttcttg
      901 cgcagctcga ggaactcctc gatgtccagg tgccacgtct cgaggtacgc gcagaccgcg
      961 cccttgcgct tgccgccctg gttgacggcg acggcggtgt cgttgaccac cttcaggaac
     1021 ggcacgacgc cctggctctt gccgttggtg cccttgatgt aggagccgag cgcccggacg
     1081 ttggtccagt cgttgcccag gccgccggcg aacttggaca gcagcgcgtt ttccttcagc
     1141 gcctcgtaga tgccgtcgag gtcgtcggcc acggtcgtca ggtagcagct ggacagctgc
     1201 gagcggcggg tgcccgagtt gaacagcgtg ggcgtggacg acatgaagtc gaaggtcgac
     1261 agcacgttgt agaactcgat cgcgcgcgcc tcgcggtcga tctcgcccag cgccaggccc
     1321 atggccacgc gcatgaagaa cgcctgcggc agctcgatgc ggacgtcgtt ctcgtgcagg
     1381 aagtagcggt cgaacagggt ctgcaggccg aggtagtcga actgcatgtc gcgcgacgcg
     1441 tcgagcgcgg cgcccagcct ggccaggtcg aactgcgcca gcttctcgtc cagcagctcg
     1501 gccttgatgc cgcgcttgat gaacctgggg aagtactcgg cgtagcgctc gcccatctgc
     1561 tcctgcgaca cctcctcgcc caggatctcc ttgcggatcg tgtgcatcag caggcgcgcg
     1621 gtggccttgg tgtaggccgg ctccttctcg atgagcgtgc gcgaggccag gatggcggcc
     1681 ttgaagacct cgtccatcgg cacgccgtcg tacaggttgc ggcgcgtctc ggcgaacacc
     1741 ggctcggcca cgacgtcggc gccgaggttg gcgcaggcgg cctcgatcag cgaacgcagg
     1801 tgggcgacgt ccaggggcac gcgctcgccc ttgtcggtga cgaacagctc gggctcggcg
     1861 gcgcgggtcg gcacctcgcc ggccttgatg cgatcctgcg cgcggcgctc gcggtacagc
     1921 acgtaggcgc gcgccacttc gtggtggctg ccgcgcatca ggcccagctc gacctggtcc
     1981 tggatgtcct cgatgtggaa ggtgccgccg gccgggcgcg agcgcagcag cgcgcgcacg
     2041 acgctctcgg tcaggccgtc gaccgtctcg cgcacgctgg ccgaggccgc gccctgcgtg
     2101 ccgtgcacgg ccaggaacgc cttcatcagc gcaacggcga tcttgctggg ttcgaagctc
     2161 accaccgcgc cgttgcggcg gatgatctgg tagccctggt aggcggactg ggagccgacg
     2221 accgtctggg caggcgcggc gtgcactgcg gccccggtct cattgatggt ggatgcgatt
     2281 ccctgcatgg cgacccctct tgctgaaatt tgtcttgaac ggaaaacctg tggatgaccg
     2341 gtgggcttcc ggtggagaac ctgtgatttc caacgcacaa agccgcggcc cgactcgcgg
     2401 tttccgcgag ctcgttgcta cgactttggg tggcgctatc cgctcggcac tgccgtcgac
     2461 tccaccctcc cttgcgatga ctgccggccg atgccggctg cccgttcgcg ggatgggtcg
     2521 attgggatga gcatgaaggt tctgctacca gttgaccaac tagtcaggtg aacgacggcg
     2581 tcgttcaacc gatggagcca ctgtatcagc taggcgatgg caacgctata cgtagcgtta
     2641 aaaaacctcc taggcactac ccgtagtgtc acgatcgacg gggagaattc tcgacgcgag
     2701 gcccggcttt cgctgcggcg catcacggac gggtgcggtc caccagcccg cgccgttgcg
     2761 cgcatcgtgc atcgcgaccc gcatggatgc tggtcccgga ccgcattgcg ggtccgcgac
     2821 ccgcatggat ccaggcctgc gcgacgagcg ggattcagtc gccgcgcggt ttcgccggcg
     2881 ggatgcgttc gtcgtcccaa tgcaactgcg acgcgaggcg cggccagtcg aatcccgggc
     2941 ccggatcgat cttgcgtccg ggcgcgacgt gctcgtggcc gacgacgtgg cggatgccgc
     3001 ggacgcgatc gatcgccggc agcagcctcg cgagcgcgtc gtactgcgcg ggctcgaagg
     3061 tgtcgccttc gagtccctcg agctcgacgc cgatcgacca gtcgttggcc tcggagcggc
     3121 cggcccagct cgagcggccg gcgtgcgacg cacggtcgtc gatcgagacg aactgcaaca
     3181 ccgagccgtc gcgccgcacg aggaaatggg ccgacacctc cagcccgcgg atcgtctcga
     3241 aatagggatg cgccgaccag tcgagcgtgt tcgtgaacag gcgctcgacc ccatcgcccc
     3301 cgtactcgcc cggaggcagg ctgatcgaat gcagcaccac ggtgtcgacg cgggcgtcgc
     3361 cgggccgcgc cctgaagttc ggggacggca cgcgccgggc gtggcgccac cagccatcgt
     3421 cccagtcgga atgcatcagg acatctccgt gccgcgcact gcgaggcgag cgccttcggg
     3481 cggccgggcg tcgctcatgt cgggtccgac gcctcggact cggtgatgcc gagccgcgcc
     3541 atccggtagc gcatctggcg cagcgacagc cccaggctgg cgccggccgc ggtgcggttg
     3601 aagttctggc gctcgagcgc gcgcacgagg atctcgcgct ccacctcgtc caggtagcgc
     3661 acgaggtcag ccggcaccgg ctcggatccg gcggcgggcg cggcgggcac ggccgccccg
     3721 atggcctcgg ccgccggcgg gccgtcgtgg tcgtcgacca ccacggcggc gatgacgtcg
     3781 gcgtccgggt cgcgcggcgg cggcgcgggg cgggtgtcgg ccagcagcgc ctcgggcagc
     3841 gcgaggtcgg ccggcacgat catctcgccg tcgctgagcg ccaccgcgcg gtgcagcagg
     3901 ttctcgagct cgcgcacgtt gccggggaac gcgtagcgcg ccagcagcgc gcgggcgtcg
     3961 ggcgacagcc gcgccggcgc cacgcccacc tcgcgcgcca gccgcgcgag cagcgcgtcg
     4021 gcgatggccg gcaggtcgac caggcgctcg cgcaggggcg gcaccttgat gtcgatcacg
     4081 ttgagccggt agaacaggtc ctggcggaag cgcccggcct gtacctcggc ggcgaggtcc
     4141 ttgtgcgtgg cgctgacgat gcgcacgttc atcgtctgct cggaggtggc gccgatcgcg
     4201 cgcaccgcgc gctcctggat cgcgcgcagc agcttggact gcatcgcgaa cggcaggtcg
     4261 ccgatctcgt cgaggaacag cgtgccgccg ttggccgcct ggaagaagcc gtcgcggtcg
     4321 gcgctggcgc ccgtgaaggc gcccttgcgc acgccgaaga actcggcttc gagcagtgtc
     4381 tcggggatgg cgccgcaatt gacggcgacg aagggctggc cggcgcgggc gctgacgtcg
     4441 tggatggcgc gcgcgacgag ctccttgccg gtgcccgact cgccctgcac cagcaccggc
     4501 gccatgctgc gcgccacctt ctcgaccagc ccgcgcacgt tggccatcgc gtcggagctg
     4561 ccgacgagcc ggcgcagcgg atcgcccttg ccgtcggacg gcgtcgcgtc gcccgcgggc
     4621 ggcgccggcg tggcgcgcga gcgtcccatc gccgacgcga ccaccgcgcg gaactggcgc
     4681 aggtcgacgg gcttggtcag gtagtcgaac gcgccggcct tgagcgccgt gacggcgttc
     4741 tcgggcgagc cgtaggcggt gacgacgatc accttctcgg gccggccgct ctgctccagc
     4801 cactgcagca ggtccaggcc ggtgccgtcg ggcagccgca tgtcgctgat gacagcgcgg
     4861 aacaggcgcg tctgcaggat ctcgcgcgcc tgggcgacag tggccgcggt ctcgacctcc
     4921 aggccctcgc gcagcagcgt cag
//
LOCUS       DATCRO010000645         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_794975, whole genome shotgun sequence.
ACCESSION   DATCRO010000645 DATCRO010000000
VERSION     DATCRO010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794975"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VIN75_17650"
     CDS             <1..1385
                     /locus_tag="VIN75_17650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976046.1"
                     /translation="ALLRQALERSAAPSDSAAPLVDGALQMLPLVHGDRFLGALWIDG
                     ELTPMSAALLDEVAARAAIAFATASLYNALQLEIAERRQAEARLEEEARRKDEFLAML
                     AHELRNPLAPIRNAVELIRLAAPTETKVRWAADVTDRQVRQLTRLVDELLDVARISQG
                     KVVLQSQCLDLVTLVTQSIDTQREAMHKRHQTLTLSLPAKPINLNGDPTRLAQVVNNL
                     LSNAIKYTPEGGAISVTVAHENTHGGEFALLEVRDNGIGIDADLLPHVFELFEQGKRA
                     LDRTQGGLGVGLTLVQRLVQLHGGDVVASSAGPGQGSQFRVLLPCLGEDDVAVVPEQE
                     GGRLPAAAISRRILVVDDNIDVVETTTMLLSLSGHQVRSAKDGLQALHVASEFRPDVV
                     LLDIGLPLMDGYEVARRLRQTPQTAGALLIALTGYGQQGDRQRGKDAGFDGHMLKPVD
                     PHALARMIEG"
     gene            complement(1417..1854)
                     /locus_tag="VIN75_17655"
     CDS             complement(1417..1854)
                     /locus_tag="VIN75_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976047.1"
                     /translation="MRETVLYVEDHPVNVLLMQTVFELRPELELVVAVDGESAMAATQ
                     HLKPALLLLDLRLPDCHGTELLHKMRQVPGWEEIPAIAVTAEAEFMPAGTTFCDVWHK
                     PLRVPTLLARLDRVLAPPSAARRDASPGTAARAVATFPRTSYA"
     gene            2071..2280
                     /locus_tag="VIN75_17660"
     CDS             2071..2280
                     /locus_tag="VIN75_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY8976048.1"
                     /translation="MTSHRIGSLVATLAFASSLAACAGMTPREHSTAVGAGIGAAAGA
                     VLTGTPGGTAAGAAVGGVIGNQVKR"
     gene            complement(2350..3795)
                     /locus_tag="VIN75_17665"
     CDS             complement(2350..3795)
                     /locus_tag="VIN75_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8976049.1"
                     /translation="MTHALIVEDDADSAETMAALISAQGFTVAIARTMRDARRQIVLQ
                     LPDLVLLDLQLPDGSGMDLFQDAQLVANSEIVLITGHASLESSIQALRMGAADYLVKP
                     INMKQLQGILSRVMRPSAIQAELASLTAEWEASGHFGHLWGRSPPMRRIYEQISRVAV
                     TAVSVFITGESGTGKEVVAQTVHDLSRRRKHPFLAVNCGAISPNLIESEIFGHEKGSF
                     TGADRQHQGFFERAHGGTLFLDEITEMPLELQVKLLRVLETGMFMRVGSTVAQEADVR
                     VIAATNRPPEQAVASGKLREDLLYRLNVFPIELPPLRDRAEDVPLLAQHFLSAICERE
                     GTTKTFTPAAVQRLQAYRWPGNVRELRNVVQRAYVMASGNTINEEWLPDDPHGPNSAY
                     ATSLTAPSRTGTTPASAPAPAHPPAASGPSITIPIGTSMADAERQLILATLEHFNQQK
                     ERTAAALGVSLKTLYNRLKEYAADKVDVAPE"
     gene            4063..4335
                     /locus_tag="VIN75_17670"
     CDS             4063..4335
                     /locus_tag="VIN75_17670"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8976050.1"
                     /translation="MVHIVIWLVVGAVIGLVAGSLMRDNDDQSVFVNVIVGVVGALVA
                     GWVVAPYFGLHAGDPKVLDFGALSVALVGAIIALALLSVFRSRRRR"
     gene            4403..4750
                     /locus_tag="VIN75_17675"
     CDS             4403..4750
                     /locus_tag="VIN75_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976051.1"
                     /translation="MPAPSIRHAADPLDDIDRDNGGADTVMGRADRADLPGERSDAPR
                     PGKGDVYIEAAREVSRESLTPSQEALDADREAERVADGDPDIEMAGEDLARGPDDDDT
                     IDETERAGDDAIR"
BASE COUNT          676 a         1621 c         1771 g          714 t
ORIGIN      
        1 tcgcgttgct gcgccaggcg ctcgagcgtt ccgcggcgcc gagcgactcc gcggcgcccc
       61 tcgtcgacgg cgcgctgcag atgctgccgc tggtgcacgg cgaccgcttc ctcggcgcgc
      121 tgtggatcga cggcgagctc acgcccatga gcgcggcgct gctggacgaa gtcgccgcgc
      181 gcgccgcgat cgcgttcgcc acggccagcc tgtacaacgc cctgcagctc gagatcgccg
      241 agcggcgcca ggccgaggcg cggctggagg aggaggcccg gcgcaaggac gagttcctgg
      301 cgatgctcgc gcacgagctg cgcaatccgc tcgcgccgat ccgcaacgcg gtcgaactga
      361 tccgcctggc cgcgcccacc gagaccaagg tgcgctgggc cgccgacgtc accgaccgcc
      421 aggtgcgcca gctcacgcgc ctggtcgacg agttgctgga cgtcgcgcgc atcagccagg
      481 gcaaggtcgt cctgcagtcg cagtgcctgg acctcgtgac gctggtgacc cagagcatcg
      541 acacgcagcg cgaggccatg cacaagcgcc accagacgct gacgctgtcg ctgccggcca
      601 agccgatcaa cctcaacggc gacccgaccc ggctcgcgca ggtggtcaac aacctgctgt
      661 ccaacgcgat caagtacacg cccgagggcg gggcgatctc ggtgacggtg gcacacgaga
      721 acacccatgg cggcgagttc gcgctgctgg aggtgcgcga caacggcatc ggcatcgacg
      781 ccgacctgct gccgcacgtg ttcgagctgt tcgagcaggg caagcgcgcc cttgaccgca
      841 cgcagggcgg cctgggcgtg ggcctgacgc tggtgcagcg cctggtgcag ctgcacggcg
      901 gcgacgtggt ggccagcagc gccgggccgg gccagggctc gcagttccgc gtgctgctgc
      961 cgtgcctggg cgaggacgac gtcgcggtgg tgccggagca ggagggcggc cggctgccgg
     1021 cggccgcgat ctcgcgccgc atcctcgtcg tggacgacaa catcgacgtc gtggagacga
     1081 cgacgatgct gctgtcgctc tcgggccacc aggtgcgcag cgccaaggac ggcctgcagg
     1141 cgctgcacgt ggcgtccgag ttccggcccg acgtggtgct gctggacatc ggcttgccgc
     1201 tgatggacgg ctacgaggtg gcgcgccgcc tgcgccagac gccgcagacc gccggcgcgc
     1261 tgctgatcgc gctgaccggc tacggccagc agggcgaccg ccagcggggc aaggatgcgg
     1321 gcttcgacgg ccacatgctc aagccggtcg acccgcacgc cctggcgcgc atgatcgaag
     1381 gctgaaagag cccggccgct tgggcggccg ggacgttcag gcgtagctgg tgcgcggaaa
     1441 ggtcgccacc gcgcgggcgg cggtgcccgg ggacgcgtcg cgtcgcgcgg ccgacggcgg
     1501 ggcgaggacg cggtccaggc gggccagcag cgtggggacg cgcagcggct tgtgccacac
     1561 gtcgcagaac gtggtgcccg ccggcatgaa ttcggcctcg gcggtgacgg cgatggcggg
     1621 gatctcctcc cagccgggca cctggcgcat cttgtgcagc agctcggtgc cgtggcagtc
     1681 gggcaggcgc aggtccagca gcaggagcgc gggcttgagg tgctgcgtgg cggccatggc
     1741 cgattcgccg tcgacggcga ccaccagctc gagctcgggc cgcagctcga acaccgtctg
     1801 catcagcagc acgttgactg gatgatcctc gacgtacagc accgtttctc gcatggcatt
     1861 cccctgatgt tggctggctt ggccagtctc gtgcacccct tcccgaactg gcgagccagc
     1921 aaacgccatg cccaccgtcc tgtcgcattt cacgccacgc ggcgaagcgg gccggaacga
     1981 acgtcggcaa cacttgcgcg acggcggccg cggtacggca cttgcctgga aagggcatcg
     2041 ggcccatgcc cgcaacaagc aaggaccacc atgacttcgc atcgaatcgg cagcctcgtc
     2101 gccaccctgg ccttcgcctc cagtctcgcc gcctgcgccg gcatgacgcc gcgcgagcac
     2161 agcacggccg tgggcgcggg catcggcgcc gccgccggcg ccgtcctgac cggtacccct
     2221 ggcggtaccg ccgcgggcgc cgcggtcggt ggcgtgatcg gcaatcaggt caagcgctga
     2281 tctgcctttc catcaatgaa aacggcaccc gcgggtgccg ttcttcttgt ccgcaggacc
     2341 caggcgggat cactccggcg ccacatcgac cttgtcggcc gcgtactcct tgaggcggtt
     2401 gtacagcgtc ttcaggctca cgcccagcgc cgcggcggtg cgttccttct gctggttgaa
     2461 gtgctcgagc gtggccagga tcagctggcg ctccgcatcg gccatcgagg tgccgatcgg
     2521 gatggtgatc gacgggccgc tggcggccgg cgggtgcgcg ggcgcgggcg ctgacgcggg
     2581 cgtggtgccg gtccggctcg gggcggtcag cgaggtggcg tacgcgctgt tggggccgtg
     2641 cgggtcgtcg ggcagccatt cctcgttgat cgtgttgccc gaggccatca cgtacgcgcg
     2701 ctggacgacg ttgcgcagct cgcgcacgtt gccgggccac cggtaggcct gcaggcgctg
     2761 cacggcggcg ggcgtgaacg tcttggtggt gccttcgcgc tcgcagatgg ccgacaggaa
     2821 atgctgggcc agcagcggca cgtcctcggc gcggtcgcgc agcggcggca gctcgatggg
     2881 gaagacgttc aggcggtaca gcaggtcctc gcgcagcttg ccggaggcca ccgcctgctc
     2941 gggcgggcga ttggtggcgg cgatgacgcg cacgtcggct tcctgcgcca ccgtggagcc
     3001 gacgcgcatg aacatgcccg tctcgagcac gcgcagcagc ttgacctgca gctccagcgg
     3061 catctcggtg atctcgtcca ggaacagcgt gccgccgtgg gcgcgttcga agaagccctg
     3121 gtgctggcgg tcggcgccgg tgaagctgcc tttctcgtgg ccgaagatct cgctctcgat
     3181 caggttgggc gagatggcgc cgcagttgac ggccaggaac gggtgcttgc ggcggcggct
     3241 caggtcgtgc acggtctggg cgacgacttc cttgccggtg ccgctctcgc cggtgatgaa
     3301 caccgagacg gccgtgacgg ccacgcgcga gatctgctcg tagatgcgcc gcatcggcgg
     3361 cgagcggccc cagaggtggc cgaagtggcc ggaggcctcc cattcggcgg tgagcgaggc
     3421 gagttcggcc tggatggccg agggccgcat gacgcgcgac aggatgccct gcagctgctt
     3481 catgttgatc ggcttgacga ggtagtcggc tgcgcccatg cgcagggcct ggatcgagct
     3541 ctccaggctg gcgtggccgg tgatcagcac gatctccgag ttggccacga gctgggcgtc
     3601 ctggaacagg tccatgccgc ttccatcggg cagttgcagg tccagcagca ccaggtcggg
     3661 cagctgcagc acgatctggc ggcgggcatc gcgcatcgtc cgggcgatcg cgacggtgaa
     3721 gccctgcgcg gagatcaggg ccgccatcgt ctcggcggag tcggcgtcgt cttcgacgat
     3781 caacgcatga gtcatggtag ttcggtgtct tgctgtcgtt cggctggcgg agggggcggg
     3841 gcggacctcc acaccgcgag gtcgcatcgg cgtcgacgcg cctcgaggga agcttgcgcc
     3901 gagtcgggac cgattctgcc cacggggggg ccgcacgctt gtaagacgat agctcgaccg
     3961 cggcgggatc tgcacctacg gttcgtgggg ttgctctgcg ggaacggcgt ttgctgccgg
     4021 gcaggccgcc gcgggcgtcg tggtcgtcga ggattcgctt acatggttca catcgtcatc
     4081 tggctggtcg ttggcgcggt catcggcctg gtcgcaggct cgttgatgcg ggataacgat
     4141 gaccagagcg tgttcgtcaa cgtgatcgtc ggcgtggtgg gggcgctcgt cgcgggctgg
     4201 gtcgtggcgc cgtatttcgg gctgcatgcc ggcgacccga aggtgctcga tttcggcgcg
     4261 ctgtcggtgg ccctggtggg cgcgatcatc gcgctggcgc tgttgagcgt gtttcggagc
     4321 cgccgccggc gctagccgcc ggcacgccag tggcatggcg gttgccgaac cgggccgtag
     4381 caacagcccc gggagatcca agatgcccgc accttccatc cgacacgccg ccgatccgct
     4441 ggacgacatc gaccgcgaca acggcggcgc cgacacggtc atgggccgcg cggatcgcgc
     4501 cgacctgccc ggcgagcgct cggacgcccc ccgtcccggc aagggtgacg tctacatcga
     4561 ggcggcgcgc gaggtctcgc gcgaatcgct gacccccagc caggaggcgc tcgacgccga
     4621 tcgcgaggcc gagcgcgtcg ccgacggcga tcccgacatc gagatggccg gcgaggacct
     4681 cgcccgcggc ccggacgacg acgacacgat cgacgagacc gagcgcgccg gcgacgacgc
     4741 gatccgctga cgggcgccgg gcccgcccgc ggccccgtgc cc
//
LOCUS       DATCRO010000646         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_480046, whole genome shotgun sequence.
ACCESSION   DATCRO010000646 DATCRO010000000
VERSION     DATCRO010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480046"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..869)
                     /locus_tag="VIN75_17680"
     CDS             complement(99..869)
                     /locus_tag="VIN75_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY8976052.1"
                     /translation="MATVLVPLPRIDFDPTEAGVPWRVLTEAGHAFVFATPDGAPAQA
                     DPRMLHGTGLGLLAPMLRAQGNAVADHDRMAQSAAYRAPLRWSEIADGVEAGRFDALL
                     LPGGHAPGMREYLESPLLQAAVAGFFERDRPVAAICHGTVLAARSRVAREGEEGRSVL
                     YGRRTTGLTRQLEMLAWRLTRHRLGDYYRTYPQTVQDEVAAALRSPRDFLAGPPALLR
                     DSDRLQRVGFTVRDGNYLSARWPGDAHRFAHELAGMLA"
     gene            complement(871..1203)
                     /gene="cyaY"
                     /locus_tag="VIN75_17685"
     CDS             complement(871..1203)
                     /gene="cyaY"
                     /locus_tag="VIN75_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY8976053.1"
                     /translation="MQDTEYQAAAERVLAAIERQCDAWLQQGVIDIDTNRAGGLVELE
                     FPNGSKIVVNKQPPLHEIWLAARNGGYHFKFADQAWRDTRDGADFFARLSDEASRQAG
                     QPLAFAAA"
     gene            1298..1498
                     /locus_tag="VIN75_17690"
     CDS             1298..1498
                     /locus_tag="VIN75_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY8976054.1"
                     /translation="MAACAAALVLALAACGQKGPLYLPSQAGTAARPVARLPSAPDDG
                     RPATPAAPVGGASSPGLPPAAN"
     gene            complement(1495..2535)
                     /locus_tag="VIN75_17695"
     CDS             complement(1495..2535)
                     /locus_tag="VIN75_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8976055.1"
                     /translation="MNDARAKAPQRPATAATVVVEARGLAKSFDLRRPLAERLLRRDA
                     PRTIPAVQDLSFTIARGRTLALVGESGCGKTTLARLMVGLEAPTSGSAWVEGVDVHAT
                     LAGPAEAARALRRRMQMIFQDPFASLDPRWRIGDIVAEPLLEQGLILPAQAPERVAGL
                     LASVGLAGVDVRRFPHQFSGGQRQRISIARAIATSPQFLVCDEPTSSLDVSVQAQVLN
                     LLADLQRERALTMLFISHNLVVVRYVADDVGVMYMGRLVELAPRGELFGAPRHPYTRM
                     LLAAVPDVHGTRRERAPVEGEASDPLAPPPGCAFHPRCPHANERCRVERPELRDSGEV
                     RVACHAVEEGRL"
     gene            complement(2532..2897)
                     /locus_tag="VIN75_17700"
                     /pseudo
     CDS             complement(2532..2897)
                     /locus_tag="VIN75_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
BASE COUNT          400 a         1096 c         1118 g          356 t
ORIGIN      
        1 cgcagccgca gagcaccgac gacgagaaga agagcatcct cgatctcttc aagaccggca
       61 actgagcgca cgcgcacacg cggcgcgcag cgagtcgctc aggccagcat gccggccagc
      121 tcgtgcgcga agcggtgggc gtcgccgggc cagcgcgcgg acaggtagtt gccgtcacgc
      181 acggtgaagc ccacgcgctg caggcggtcg gaatcgcgca gcagcgccgg cgggccggcc
      241 aggaagtcgc gcggcgagcg cagcgccgcg gccacctcgt cctgcaccgt ctgcggatac
      301 gtgcggtagt agtcgccgag gcgatgccgc gtgaggcgcc aagccagcat ctcgagctgg
      361 cgcgtcaggc ccgtggtgcg ccggccgtac agcacgctgc gcccttcctc gccctcgcgc
      421 gcgacgcgcg agcgcgcggc gagcacggtg ccgtggcaga tggcggcgac ggggcggtcg
      481 cgttcgaaga acccggcgac cgcggcctgc agcagcggcg actcgaggta ctcgcgcatg
      541 ccgggcgcgt ggccgccggg cagcagcagc gcatcgaagc ggcctgcctc gacgccgtcg
      601 gcgatctcgc tccatcgcag gggcgcgcgg tacgcggccg actgcgccat gcggtcatgg
      661 tcggccaccg cgttgccctg cgcgcgcagc atcggcgcca gcagccccag cccggtgccg
      721 tgcagcatgc gcgggtcggc ctgcgccggg gcgccgtccg gcgtggcgaa gacgaaggcg
      781 tggccggcct cggtcagcac gcgccagggc acgcccgcct cggtcgggtc gaagtcgatg
      841 cgcgggagcg ggacgaggac ggtggccacg tcaggccgcg gcgaaggcca gcggctggcc
      901 ggcctggcgg ctcgcctcgt cggacaggcg ggcgaagaaa tcggcgccgt cgcgcgtgtc
      961 gcgccaggcc tggtcggcga acttgaagtg gtagccgccg ttgcgcgcgg ccagccagat
     1021 ctcgtgcagc ggcggctgct tgttgacgac gatcttgctg ccgttcggga actcgagctc
     1081 caccaggccg ccggcgcggt tggtgtcgat gtcgatgacc ccttgctgca gccaggcgtc
     1141 gcactggcgc tcgatggcgg ccagcacgcg ctcggcggct gcctggtact cggtgtcctg
     1201 caaggtcggg gccgacatgg tcgatagaat ccgtgggatg aatggaagcc agggatcgag
     1261 tctagcggag cagcgccgcc ctgcgcgcgg tggagtcttg gccgcgtgcg cagcggcgct
     1321 cgtgctcgcg ctggcggcgt gcggccagaa ggggccgctg tacctgccgt cccaggccgg
     1381 cacggcggcc aggccggtgg cgcgcctgcc gagcgcgccc gacgatggcc gcccggccac
     1441 gcccgccgcg ccggtcgggg gcgccagctc gccgggcctg cccccggccg cgaactagag
     1501 ccgcccctcc tcgaccgcgt ggcacgccac gcgcacctcg cccgaatcgc gcagttccgg
     1561 ccgctcgacg cggcagcgct cgttcgcgtg cgggcagcgc ggatggaagg cgcagcccgg
     1621 cggcggcgcc agcggatccg aggcctcgcc ttcgaccggc gcgcgctcgc ggcgcgtgcc
     1681 gtgcacgtcg ggcacggcgg ccagcagcat gcgcgtgtac ggatggcgcg gcgcgccgaa
     1741 gagctcgccg cgcggcgcca gctccaccag ccgccccatg tacatcacgc cgacgtcgtc
     1801 ggccacgtag cgcacgacca ccaggttgtg cgagatgaac agcatcgtca gcgcgcgttc
     1861 gcgctgcagg tcggccagca ggttgagcac ctgggcctgc acggacacgt ccagcgacga
     1921 cgtcggttcg tcgcagacga ggaactgggg gctggtcgcg atggcgcgcg cgatcgagat
     1981 gcgctggcgc tggccgccgg agaactggtg cgggaaccgg cgcacgtcga cgccggccaa
     2041 tcccaccgac gccagcagcc cggccacgcg ctcgggcgcc tgcgccggca ggatcaggcc
     2101 ctgctcgagc agcggctcgg ccacgatgtc gccgatgcgc cagcgcgggt ccaggctggc
     2161 gaacggatcc tggaagatca tctgcatgcg gcggcgcaag gcgcgcgcag cctccgccgg
     2221 tccagccagc gtcgcgtgca cgtcgacgcc ctccacccac gcgctgccgc tcgtgggcgc
     2281 ctccaggccg accatcaggc gcgccagcgt cgtcttgccg cagcccgact cgcccaccag
     2341 cgccagcgtg cggccgcgcg cgatcgtgaa gctgaggtcc tgcacggcgg ggatcgtgcg
     2401 cggcgcatcg cggcgcagca ggcgctcggc cagcggacga cgcaagtcga aggacttggc
     2461 cagcccgcgc gcctcgacca ccaccgtggc ggccgtggcg gggcgctgcg gcgccttcgc
     2521 tcgcgcgtcg ttcatgccgc gggcccttcc acgatgggcc accagcaggc cgcgcgcgtg
     2581 cgccgcacca gggccagctc gggtcgctcc tcggcgcagc gcgggctcgc cttcgggcag
     2641 cgcgggtgga acgcgcaacc gggcgggatc gcgtcgaggt cgggcaacgc gccggcgatc
     2701 tgcgccagcc gccggccgtc gccgtccagc gtgggcaccg acgccatcag gccggccgtg
     2761 tacggatgcg cgggccggcg gatcacctcg tcgacaggcc cgatctcgac gatgcgtccg
     2821 gcgtacatca ccgccacgcg gtcgcaggtc tcggcgatga cgcccatgtc atgcgtgatc
     2881 agcatcaccg acgcgccatg cgccccggcg gttccagcag gccgatgatc gcggcgccgg
     2941 tcagcgactt gcccgcgccc gattcgccga
//
LOCUS       DATCRO010000647         7746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_592521, whole genome shotgun sequence.
ACCESSION   DATCRO010000647 DATCRO010000000
VERSION     DATCRO010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592521"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIN75_17705"
     CDS             complement(<1..453)
                     /locus_tag="VIN75_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8976056.1"
                     /translation="MTLPIDFPHISRPHLLQLLRDAPKAELHIHIEGSLEPELIFELA
                     RRNGVALPYASVDALRRAYAFTNLQSFLDVYYAGASVLITEDDFDAMAWAYFRRAAHD
                     NVVHTEIFFDPQTHTARGVDMGVVIRGLDRAARRAEAELGLSVRLILCF"
     gene            696..1484
                     /locus_tag="VIN75_17710"
     CDS             696..1484
                     /locus_tag="VIN75_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8976057.1"
                     /translation="MTFDLMHAVAGLLVGAIVGVTGVGGGALMTPLLVMMFGVAPTTA
                     VGTDLLYASITKSFGTAVHHQAGTIDWQVVRRLALGSLPAAALTLAWMHVEHIAQLRS
                     GLITTTLGIALVVTAVGILFKEQIHSMGTRFRLGDSARFKAMQPWLTMACGVLLGAMV
                     TLTSVGAGALGTAMLVYLYPLRLSAGKLVGTDLAHAIPLTLIAGLGHLALGSVDPVLM
                     LNLLAGSIPGVLAGSWISTRAPVGPLRTAIAVVLLFVAWKMVTR"
     gene            1778..2560
                     /locus_tag="VIN75_17715"
     CDS             1778..2560
                     /locus_tag="VIN75_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8976058.1"
                     /translation="MNRPLVAVLAAALSLVATTAGASAVTSVQLSDFQFRLIDLDPSD
                     GITPSIDLSAYPGSLVYASGYFDQGSSVFGPAQISASFESGFLEQASITNDLTDGGTA
                     TALASVSDATKNYRFAESTVILGVQNSTALFTLSPHTELVMSGTVDMTATTSQVDGFT
                     GSADALAEFAFSTATSGFNDIDTDFAVIDIRGDSDRSLQSDLSVTLSNTSDASMQGGF
                     YVRLSSDAGIVPIRTPVPEPGNVALMLAGLAGLVPLARRRRN"
     gene            complement(2598..3362)
                     /locus_tag="VIN75_17720"
     CDS             complement(2598..3362)
                     /locus_tag="VIN75_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976059.1"
                     /translation="MLISVVNRSTKVSDAELQAAVRAINRQLEEDFYPHWQFGARLRV
                     DSAGRVPRSRERKVDLPQLPGRRGDAVIYLVDHPTMAQTEGYHDSNNLDVPFGFVFLD
                     ACADGADCWTVALSHEAIELVGDPLSNLLVQGPHPEDRRHLVFHQYELCDAVSGEFYE
                     IEGVKVQNFVLPGWFSRKVVKGARNDFLGRPRSGSSLRPFSVAPGGYLLFWDDRKPEG
                     RKWTPFYDKRDGMAERKVQEKLANKLSRMSRRERGK"
     gene            3476..4390
                     /locus_tag="VIN75_17725"
     CDS             3476..4390
                     /locus_tag="VIN75_17725"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEY8976060.1"
                     /translation="MDYADVLRQIAHELQPVIGQGQVASYIPQLASVSPRHFGMAVVT
                     ITGQVHTVGDAHHAFSIQSISKLFTLVMALQLEGEDVWKRVGREPSGTPFNSLVQLEA
                     ENGRPRNPFINPGALVCTDILCTRFATPENAVVEFLRPLCGNRDVHYNRAVAASERRT
                     SHRNAAMAHLIKSFGNLANSVDDVLDAYCRHCAIEMNCVELARAVAFLAHGGINPADG
                     SAVLGPSLTKRLNALMLTCGTYDAAGDFAFRVGLPAKSGVGGGIVAVMPGECGICVWS
                     PGLEPSGNSLAGSLALERFTTLTGRSIF"
     gene            complement(4617..5885)
                     /locus_tag="VIN75_17730"
     CDS             complement(4617..5885)
                     /locus_tag="VIN75_17730"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8976061.1"
                     /translation="MTDLLDPLLDATFKGFPHGAAPLRRSQVGAQGWNVLAGDLPLPL
                     AVIRRDALAHNVGWMQRFADAAGVGFAPHGKTTMSPQLFRRQLDAGAWGLTFATVTQM
                     QAGLAAGVRNVLIANQVLDAADLGAIAALADTHPGVRVVFLLDSLAQLELIEDVSSDR
                     PFEVLVEVGVAGGRTGVRSHADALALARRAHASGAVSLAGIECYEGLGSMVGGEGDAP
                     AVADLMDRVERLMRECDAEGLFDVDEAIVSAGGSAVFDLVVPRLRVELTRPVRGLLRS
                     GCYVTHDQGHYTRLVRAANRRLGCDDAQGLRAALEVWALVQSMPEPGLAILAVGKRDI
                     SFDLGLPLPLAVVARASRERVPAPAAWAVSKLNDQHAYLRLGDAAGGLQVGDRVCLGL
                     SHPCTTFDKWRWMPVVDEGGGVVDAIVTWF"
     gene            6027..6689
                     /locus_tag="VIN75_17735"
     CDS             6027..6689
                     /locus_tag="VIN75_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin"
                     /protein_id="HEY8976062.1"
                     /translation="MTRTTLSFVAAGSLLLALAMPARSASVTVTVHGADGQPAPNVVV
                     QLVPAQPGPARPPWTPVVVVQRDIHFVPAITAVPVGTTVRFSNQDPYDHHIRSEPAGP
                     LGNIPPAKAFEMRLAGASGDKVASADVTLDKPGLIVLGCHLHGSMRGHVYVSSTPWVA
                     VTDASGAATIADVPDGAVELRTWSPEQLVDQPALQRQVGGSSVILDATLNFTPPKRRR
                     HS"
     gene            6705..7637
                     /locus_tag="VIN75_17740"
     CDS             6705..7637
                     /locus_tag="VIN75_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HEY8976063.1"
                     /translation="MPLTDTRPGVDRRLPASGALLVAAALALAHPRAHADSDKLPLTG
                     GVTSIDGAAGGGLTPWAVTGSNATEGQWGAAATASVVSTRNYSMRVAGALVAWDDRAE
                     LSVAHQDFTTGATGRALGLPGLHLKMDVVGAKVRLLGDAILDSDTWVPQVAAGVQIKR
                     TDAGALKPTLQALGARSGGVDVYLSATKLLLAQGVLVNATLRATKANQNGLLGFGGTA
                     QEHYRVEPEFSVAWLANRRLALGAEYRRKPDNLDPSVLGAGLKEDDWWDVFAAWAPCR
                     GFSLTAAYVQLGRIVPAVATKRQDGGYLSAQLAF"
BASE COUNT         1126 a         2827 c         2647 g         1146 t
ORIGIN      
        1 gaagcacagg atcaggcgca cgctcaggcc cagctcggcc tccgcgcgcc gggccgcgcg
       61 gtccaggccg cggatgacga cgcccatgtc gacgccgcgc gccgtgtggg tctgcgggtc
      121 gaagaagatc tcggtgtgga cgacgttgtc gtgcgcggcg cggcgaaagt acgcccacgc
      181 catcgcgtcg aagtcgtcct cggtgatcag cacgctggcc ccggcgtagt agacgtccag
      241 gaagctctgc aggttggtga acgcatacgc ccggcgcagc gcgtccacgc tggcgtacgg
      301 cagcgccacg ccgttgcgcc tggccagctc gaagatcagc tcgggctcca gcgacccctc
      361 gatgtggatg tgcagttcgg ccttgggcgc gtcgcgcagc agttgcagca ggtgggggcg
      421 agagatgtgg gggaagtcga tgggcagggt catggccgtt ttccgaggcg ggtttgggcc
      481 gactttaagc cgtcggtcgg gcgggcggga accgaattgc cggcgcggcg gctatgcatc
      541 ctcggttttc gcataacggc cgacgccgtc tgatcggaaa atgttgggtg gacctgcatt
      601 ccccgcgtgc cgtcccacgc cgcagtttcc ttcccgtctc tgtcgcgcgc gacgcgcggc
      661 gtcccgatca ctcccgtctc cttgcaggcc cagtcttgac cttcgacctg atgcatgccg
      721 tcgccggcct gctggtgggc gccatcgtcg gcgtcaccgg cgtgggcggg ggcgcgttga
      781 tgacgccgct gctggtgatg atgttcggcg tcgccccgac caccgccgtc ggcaccgacc
      841 tgctgtacgc ctccatcacc aagagcttcg gcaccgccgt ccaccaccag gcaggcacca
      901 tcgactggca ggtggtgcgc cggctcgcac tgggcagcct gcccgccgcg gcgctgacgc
      961 tcgcgtggat gcacgtcgag cacatcgcgc agctgcgcag cgggctcatc acgacgacgc
     1021 tgggcatcgc gctcgtcgtg acggccgtcg gcatcctgtt caaggagcag atccactcga
     1081 tgggcacgcg cttccgtctc ggcgacagcg cgcgcttcaa ggccatgcag ccgtggctga
     1141 cgatggcctg cggcgtgctg ctgggcgcga tggtgacgct gacgtcggtc ggcgccggcg
     1201 cgctgggcac ggcgatgctg gtctatctct atccgctgcg gctgtcggcc ggcaagctgg
     1261 tgggcaccga cctcgcgcac gcgatcccgc tgacgctgat cgccggcctg ggccacctgg
     1321 ccctgggcag cgtcgacccg gtgctgatgc tgaacctgct ggccggctcg atccccggcg
     1381 tgctcgcggg ctcgtggatc agcacgcgcg cgccggtcgg cccgcttcgc accgcgatcg
     1441 cggtggtgct gctgttcgtc gcctggaaga tggtgacgcg ctagtgtcgt gattgggaag
     1501 ttcgttgaac gggtcccgag cgcagaggag acgttgccga atcctctcgc aaacgtccgt
     1561 gggccaggcg gcgcgcggag cgaggctcga tgcctcgcga gcacgccaac gacggccacg
     1621 ggcgttttcg aggggattct tcaacgaact tctcaaccac gacacgaggg ggacagcctg
     1681 gtctctggcg cagcgggcgc atcgattgcc ccttgcgcga ccgcgcgaag caccgccttc
     1741 gccctcgcct ggccatcctc agcaccaagg acagatcatg aatcgacctc tcgtcgccgt
     1801 actggccgcc gccctgagcc tcgtcgccac gacggccggc gcctcggctg tcaccagcgt
     1861 gcagctgtcc gacttccagt tccggctgat cgacctggat ccgagcgacg ggatcacgcc
     1921 gtcgatcgac ctgagcgcct atcccggcag cctcgtctac gccagcggct acttcgacca
     1981 gggaagctcc gtgttcgggc cggcgcagat ctcggcctcc ttcgagagcg gcttcctgga
     2041 acaggcctcg atcacgaacg acctgacgga cggcggaacc gcgacggcgc tggcctcggt
     2101 cagcgatgcc acgaagaact accggttcgc cgagagcacg gtcatcctgg gcgtccagaa
     2161 ttccacggcg ctcttcaccc tctcccctca caccgagctc gtgatgtccg gaaccgtcga
     2221 catgacggca acgacgtcgc aagtcgacgg attcaccggg tccgcggacg ccttggccga
     2281 attcgctttc tcgacggcca cgtccggctt caacgacatc gacacggact tcgcggtgat
     2341 cgatatccgc ggcgactccg atcggtcgct gcagtccgac ctgtcggtga cgctgtccaa
     2401 cacgtccgac gcgtcgatgc agggcggctt ctatgtccgc ctgtcgagcg acgcgggcat
     2461 cgtcccgatt cgcaccccgg tgccggagcc gggcaacgtg gcgctgatgc ttgccggcct
     2521 ggccggcctg gtgccgctgg cgcgtcgccg ccgcaattga cgctggcggc gcggcgcatt
     2581 gcccgcgcct cgtccggcta cttcccgcgc tcgcgccgcg acatgcggct cagcttgttg
     2641 gccagcttct cctgcacctt gcgttcggcc atgccgtcgc gcttgtcgta gaacggcgtc
     2701 cacttcctgc cctcgggctt gcggtcgtcc cagaacagca ggtagccgcc gggggccacc
     2761 gagaacgggc gcagcgacga gcctgagcgc gggcgcccca ggaaatcgtt gcgcgcgccc
     2821 ttgacgacct tgcgcgagaa ccatccgggc agcacgaagt tctgcacctt gacgccctcg
     2881 atctcgtaga actcgccgga aacggcgtcg cacagctcgt actggtggaa cacgaggtgg
     2941 cggcgatcct ccggatgcgg gccctgcacc agcaggttgc tcagcggatc gcccaccagc
     3001 tcgatcgcct cgtgcgacag ggccaccgtc caacagtcgg caccgtcggc gcaggcgtcg
     3061 aggaacacga agccgaacgg cacgtccagg ttgttgctgt cgtggtagcc ctcggtctgc
     3121 gccatcgtcg gatggtcgac gaggtagatg acggcgtcgc cgcgccgccc cggcaactgc
     3181 ggcaggtcca ccttgcgctc gcgcgagcgc ggcacgcggc ccgcgctgtc gacgcgcagg
     3241 cgcgcgccga actgccagtg cggatagaag tcctcctcca gctggcgatt gatggcgcgc
     3301 accgccgcct gcagctccgc gtccgacacc ttcgtgctgc gattgaccac cgagatcagc
     3361 atgggctccc tctccttgac tggcgtgtgg cggcgattgt gctgtttctc ccgcgacctc
     3421 gccagacgcg ccgtggcgcg atactgcggg ccgacagctt tcttcagcgc cgcccatgga
     3481 ttacgccgac gtcctccgcc agatcgccca tgaactgcag cccgtcatcg ggcaaggcca
     3541 ggtcgccagc tacatccccc agctcgccag cgtgtcgccg cggcacttcg gcatggccgt
     3601 ggtgacgatc accgggcagg tgcacaccgt cggcgacgcg caccatgcgt tctcgatcca
     3661 gtcgatctcc aagctgttca cgctcgtgat ggcgctgcag ctcgaaggcg aggacgtgtg
     3721 gaagcgcgtc ggccgcgagc cctcgggcac gccgttcaac tcgctggtgc agctcgaggc
     3781 cgagaacggc aggccgcgca atccgttcat caacccgggt gcgctggtct gcaccgacat
     3841 cctgtgcacg cgcttcgcca cgcccgagaa cgcggtcgtc gagttcctgc gcccgctgtg
     3901 cggcaaccgc gacgtgcact acaaccgcgc cgtcgccgcc tcggagcgcc gcacctcgca
     3961 tcgcaacgcc gccatggcgc acctgatcaa gtccttcggc aacctcgcca acagcgtcga
     4021 cgacgtgctc gatgcctatt gccgccactg cgccatcgag atgaactgcg tcgagctcgc
     4081 gcgcgccgtc gccttcctcg cgcacggcgg catcaacccg gccgacggca gcgccgtcct
     4141 cggccccagc ctgaccaagc ggctgaacgc gctgatgctg acctgcggca cctacgacgc
     4201 cgccggcgac ttcgcgttcc gcgtcggcct gcccgccaag agcggcgtgg gcggcggcat
     4261 cgtggcggtg atgcccggcg agtgcggcat ctgcgtctgg tcgccgggac tggagccctc
     4321 gggcaactcg ctggcggggt cgctcgcgct ggagcgcttc acgacgctca cggggcgatc
     4381 gatcttctag ggcctgttca cactcaatgc gtgcccgcgc cggctgccaa cgacgcctgg
     4441 ggcctcgcag gcagccggcc ctgcgggtga tcctccagaa agcccgcctt cggcgtcgtg
     4501 accgtaggga acgcttgccg tggcgccacc acgtcggcgc gcccgcgcct tgaaggcggg
     4561 ctttctggag gtcacgcggg cacgcattca gtatgaacag gccctagcga tcgcgttcag
     4621 aaccaggtga cgatcgcgtc gaccacgccg ccgccttcgt cgacgacggg catccagcgc
     4681 cacttgtcga aggtggtgca cgggtgcgac aggccgaggc agacgcggtc gcccacctgc
     4741 aggccgccgg cggcgtcgcc cagacgcagg taggcgtgct ggtcgttgag cttggagacg
     4801 gcccacgccg cgggcgccgg cacgcgctcg cggctggcgc gagcgacgac ggccagcggc
     4861 agcggcaagc ccaggtcgaa cgagatgtcg cgcttgccga cggcaagaat cgccaggccc
     4921 ggctcgggca tggactgcac cagcgcccac acttcgagcg cggcacgcag gccctgcgcg
     4981 tcgtcgcagc ccaggcggcg gttggccgcg cgcacgaggc gcgtgtagtg gccctggtca
     5041 tgcgtgacgt agcagcccga gcgcagcagg ccgcgcaccg gccgcgtcag ctcgacgcgc
     5101 aggcgcggca cgacgaggtc gaacaccgcg gagccgccgg ccgagacgat cgcctcgtcg
     5161 acatcgaaca ggccctccgc gtcgcattcg cgcatgaggc gctcgacgcg gtccatgagg
     5221 tcggcgaccg ccggcgcgtc gccctccccg ccgaccatgc tgcccaggcc ctcgtagcac
     5281 tcgatgcccg ccagcgacac cgcgccgctc gcgtgcgcac ggcgcgcgag cgcgagcgcg
     5341 tccgcatgcg agcgcacgcc cgtgcggccg ccggcgacgc ccacctcgac cagcacctcg
     5401 aacggccggt cgctggacac gtcctcgatg agctcgagct gcgcgagcga atccagcagg
     5461 aagacgacgc gcacgccggg gtgggtgtcg gccagcgcgg cgatggcgcc caggtcggcg
     5521 gcgtccagca cctggttggc gatcagcacg ttgcgcacgc cggccgcgag cccggcctgc
     5581 atctgcgtga cggtggcgaa cgtcaggccc cacgcgccgg catcgagctg gcgccggaac
     5641 agctgcggcg acatcgtcgt cttgccgtgc ggggcgaagc ccacgccggc ggcatcggcg
     5701 aagcgctgca tccagcccac gttgtgggcc agggcgtcgc gccggatcac cgccagcggc
     5761 agcggcaggt cgcccgcgag cacgttccag ccctgcgcgc cgacctgcga gcggcgcagc
     5821 ggcgcggccc cgtgcggaaa gcccttgaag gtggcgtcga gcagcgggtc gaggaggtcg
     5881 gtcatgcgcc gatcataggc ggcgggccgc cgatccatcg cgaaacgaga aatcgttcac
     5941 gctcgacggc gggatgttcg cgaccggctg ccgatggggc ggtatccatg cgcatccgcc
     6001 gcaagcgctc gtccgtagat cgacacatga cacgcaccac cttgtccttc gtcgccgccg
     6061 gcagcctgct gctggcgctc gccatgcccg cccgctccgc cagcgtcacc gtcaccgtgc
     6121 acggggccga cggacagccc gcgcccaacg tcgtggtgca gctcgtgcct gcgcagccgg
     6181 gcccggcgcg gccgccctgg acgccggtgg tggtcgtgca gcgcgacatc cacttcgtgc
     6241 cggccatcac ggcggtgccg gtgggcacga cggtgcgctt ctccaaccag gatccgtacg
     6301 accaccacat ccgctcggag cccgccggcc cgctgggcaa catcccgccg gccaaggcct
     6361 tcgagatgcg gctggccggc gccagcggcg acaaggtggc cagcgccgac gtcaccctcg
     6421 acaagcccgg tctcatcgtc ctcggctgcc acctgcacgg gtcgatgcgc ggccacgtct
     6481 atgtcagcag cacgccgtgg gttgccgtga ccgacgccag cggcgccgcg acgatcgccg
     6541 acgtgccgga cggcgccgtc gaactgcgca cctggagccc ggagcaactg gtcgaccagc
     6601 ccgcgctgca gaggcaggtc ggcggcagca gcgtcatcct ggacgcgacg ctgaacttca
     6661 cgccgcccaa gcgccgccgc cattcctgaa agcatggaga ccgcatgccc ctgaccgaca
     6721 cccggcccgg cgtcgaccgg cgcctgcccg cgagcggcgc gctgctggtc gccgccgccc
     6781 tcgcgctggc gcacccgcgg gcccacgcgg acagcgacaa gctgccgctg accggcggcg
     6841 tcaccagcat cgacggcgcg gccggcggcg gcctgacgcc gtgggccgtg accggcagca
     6901 acgccaccga agggcaatgg ggcgcggcgg cgacggcctc cgtcgtgtcc acgcgcaact
     6961 attcgatgcg ggtggcgggc gcgctggtgg cctgggacga ccgcgccgag ctgtcggtcg
     7021 cgcaccagga cttcaccacc ggcgccacgg gccgcgcgct gggcctgccg ggcctgcacc
     7081 tgaagatgga cgtggtaggc gccaaggtgc ggctgctggg cgacgcgatc ctcgactcgg
     7141 acacctgggt gccgcaggtc gccgcgggcg tccagatcaa gcgcaccgac gccggcgcgc
     7201 tcaagcccac gctgcaggcg ctgggcgcac gcagcggcgg tgtcgatgtc tacctcagcg
     7261 cgaccaagct gctgctcgcg cagggcgtgc tcgtcaacgc gacgctgcgc gcgaccaagg
     7321 ccaaccagaa cggcctgctg ggcttcggcg gcacggcgca ggagcactat cgcgtcgagc
     7381 ccgagttctc ggtcgcctgg ctcgccaatc gccgcctggc gctcggcgcc gaatatcgcc
     7441 gcaagcccga caacctggac ccgtccgtgc tcggcgccgg cctcaaggag gacgactggt
     7501 gggacgtgtt cgccgcctgg gcgccgtgcc gcgggttctc gctgaccgcg gcgtacgtgc
     7561 agctgggccg catcgtgccg gctgtcgcga cgaagcggca ggacggcggc tacctgtccg
     7621 cgcagctcgc cttctgaacc tttccgaagg ccaaggaatc gccatgacca agcttcttct
     7681 cgcggcgctg ctcgcggcct gcacgctcac cgccgcgcac gcgcaggcga acgcacccgc
     7741 caattc
//
LOCUS       DATCRO010000648         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_693778, whole genome shotgun sequence.
ACCESSION   DATCRO010000648 DATCRO010000000
VERSION     DATCRO010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693778"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1125)
                     /gene="sbnB"
                     /locus_tag="VIN75_17745"
     CDS             complement(97..1125)
                     /gene="sbnB"
                     /locus_tag="VIN75_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="HEY8976064.1"
                     /translation="MSDTPPQFSVLGGAFIRDVLSDSRREVIELVRECYLQHHREQTL
                     NPDSYFLRFPDKPEARIIALPAAIRPDGGRGAVSGIKWIASYPRNVEANLQRASAVIL
                     LNDYETGYPFACLEASQISAARTAASAVLAAGVLRPGERHARTLSIIGGGVIAAAILA
                     FFDADGWTFDDVRLFDLSEAHASAFIRNMGTSRRPVRGVTQHEALDADVVVLATTAGT
                     PWIPASQALRPDQLVLNVSLRDLAPEILLRANNVFDDVEHCMKANTSPHLAQQLVGHR
                     DFVTGTLAQVITGERTPDPERATIFSPFGLGVLDLKLALYLYGRRAEAGRDMVVPDFF
                     PDTQRWSA"
     gene            complement(1118..2107)
                     /gene="sbnA"
                     /locus_tag="VIN75_17750"
     CDS             complement(1118..2107)
                     /gene="sbnA"
                     /locus_tag="VIN75_17750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HEY8976065.1"
                     /translation="MKYDSIEHIISDDTFLTLHDFAPGHHIYLKMEGFNPGGSIKIKT
                     AMALIRDAEERRQLRERKRIIESSSGNLGLSISVIAAARGYRFTCVVDKNTSRQNIEL
                     MRALGTEVIVIDRRDEQGGFLGSRLAYIREAIELDPELIWLNQYQNPANPAIHAEMTA
                     RSIVREFGRVDYLFVGAGTTGTLMGCVDFFRRHAPNTRLVAVDSVGSVTFGEPARTRY
                     LPGLGASVMPHFFDAGRLDELVQIPEVETVITCRDVARRYGYLSGASTGTVLAAVRRA
                     LPGLRRGATVVTLSPDLGQGYLDTVYSDDWCDRTFGAAWRAYSIPTTKALEHV"
     gene            complement(2173..2661)
                     /locus_tag="VIN75_17755"
     CDS             complement(2173..2661)
                     /locus_tag="VIN75_17755"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY8976066.1"
                     /translation="MTRSTLPADAGHLPTDIPMNDERTTSPEVLLLPPGLLRPTEEFS
                     PDRLDALTAEILASGQWPFPILVERSSLVVMDGHHRREFALRQALQRVPCLMLDYRDV
                     ELESRRPGIGVTPDEVIARGLAGRPFPPKSTRHTLRSAPPALGSWPLDSLRAADLQAA
                     LG"
     gene            complement(2658..>4760)
                     /locus_tag="VIN75_17760"
     CDS             complement(2658..>4760)
                     /locus_tag="VIN75_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY8976067.1"
                     /translation="AAWRPDPFGRPGARLLASGERGRCRPDGSLERLGRVDGLSTVQG
                     RLLDTAVVQAALRAAPGVAEVAVLVDPAPGQDRLVACVVAQDDGSVSAEALREHLLRQ
                     LPPAMLPMRWHFVAGLPRGADGQVDRVALAALAADGAGAPAAPVAPRNALEASLASLW
                     ADLLKVPQVGVHDNFFSLGGHSLLAVRLVATLRANFAFELSLQEVFQAPTVAALAARL
                     GSRRAAGAAEAAGVPLPAGARSAWATGRPSFGQEDILRDLADDAIPGDELHGIRAAWR
                     VTGRLDLARLQAAFDHLLQRHDALRSAFVRGPDGWRVHVRENARVPFIIEDMRGIAAD
                     PHAVQARVMAALHAPIDIAQAPLMRVHVATLADDDIVLAIAVSHLVFDGESDDVFWDE
                     ATRLYDAPDSQWPTLLGEPPLQYSAFADWQRRFMRDDPLAAIQVAHWRERMRGMATTL
                     PLPCDHPRPPVFTHRGAGLSAPLDAQLVRDMREQGARHGWTRYTQVMAALFATLRVET
                     GEDDLFIVTPAGGRFASELFGSVGFYARGLVIRGQLDPHANGTALLAQVHDRVVEALS
                     NQHIPRSAIIDLCGPRPDRSHTARFQVHFSYVQTGGANPRLGGRELVPVACGTDATYN
                     DLRFVVAESTDGVTLSLQYCRDLFDAPTARRLLDRLVLAVGQLCRVRSQDLPLADVLA
                     RLETAGAPASPAAIGAPA"
BASE COUNT          689 a         1603 c         1840 g          628 t
ORIGIN      
        1 gaacgacggt ggacggcttg aaggcaaggg tcatgatcgg caaggcaaag gcaggacggg
       61 gcgcgcggcg attcccgcgg gcgcgccgcg ccgcgttcag gcgctccagc gctgggtgtc
      121 ggggaagaag tcggggacga ccatgtcgcg gccggcctcg gcgcggcggc cgtacaggta
      181 cagggccagc ttcaggtcga gcaccccgag gccgaacggc gagaagatcg tggcgcgttc
      241 cggatcgggc gtgcgctcgc cggtgatgac ctgggcgagc gtgccggtca cgaagtcgcg
      301 gtggccgacc agctgctgcg ccaggtgcgg cgacgtgttg gccttcatgc agtgctcgac
      361 gtcgtcgaac acgttgttgg cgcgcagcag gatctccggc gcgaggtcgc gcagcgacac
      421 gttcaggacg agctggtcgg gccgcagcgc ctgcgacgcc gggatccacg gcgtcccggc
      481 ggtcgtcgcc agcacgacga cgtccgcgtc gagcgcctcg tgctgcgtga cgccgcgcac
      541 cggccggcgg ctcgtgccca tgttgcggat gaaggcgctg gcgtgcgcct ccgacaggtc
      601 gaacaggcgc acgtcgtcga acgtccagcc gtcggcgtcg aagaaggcca ggatggccgc
      661 ggcgatcacg ccgccgccga tgatcgacag cgtgcgcgca tggcgctcgc cgggccgcag
      721 cacgccggcc gcgaggacgg cggaggcggc ggtgcgggcc gcgctgatct gcgaggcctc
      781 gaggcaggcg aacggatagc cggtctcgta gtcgttcagc aggatgaccg ccgacgcccg
      841 ctgcaggttg gcctcgacgt tgcgcgggta gctggcgatc cacttgatgc ccgagacggc
      901 gccgcgaccg ccgtcgggac ggatcgcggc cggcaacgcg atgatgcggg cctcgggctt
      961 gtcgggaaaa cgcaggaaat agctgtccgg attgagggtc tgctcgcgat ggtgctgcag
     1021 gtagcactcg cgcacgagct cgatgacctc gcgccgggag tcggacagca cgtcacggat
     1081 gaacgcgccg ccgaggacgc tgaattgcgg tggggtgtca gacatgttcg agggctttcg
     1141 tggtcggaat ggaataggcg cgccacgcgg cgccgaaggt gcggtcgcac cagtcgtcgc
     1201 tgtagacggt gtcgagatag ccctgcccga ggtccggcga cagcgtgacc acggtcgcgc
     1261 cgcgccgcag ccccggcagc gcgcgccgga ccgccgcgag cacggtcccg gtcgacgcgc
     1321 cgctgaggta gccgtagcgg cgcgccacgt cgcggcaggt gatgaccgtc tcgacctcgg
     1381 ggatctgcac cagctcgtcc aggcggccgg catcgaagaa gtgcggcatc acgctggcgc
     1441 ccaggcccgg caggtagcgg gtgcgggccg gctcgccgaa ggtcaccgac ccgaccgagt
     1501 cgacggccac gagccgcgtg ttcggggcat ggcggcgaaa gaagtccacg cagcccatca
     1561 gggtgccggt ggtgccggcg ccgacgaaca ggtagtcgac ccgcccgaac tcgcgcacga
     1621 tggaacgcgc cgtcatctcc gcgtggattg ccggattggc gggattctgg tactggttca
     1681 gccagatcag ctccggatcg agctcgatgg cttcgcggat gtaggcgagc cggctgccga
     1741 ggaagccgcc ctgctcgtcg cgccggtcga tgacgatcac ctcggtgccg agcgcacgca
     1801 tgagctcgat gttctggcgg ctggtgttct tgtcgaccac gcaggtgaag cggtatccgc
     1861 gcgcggccgc gatcacgctg atcgacaggc ccaggttgcc cgagctcgac tcgatgatcc
     1921 gcttgcgttc gcgcagctgg cgccgctcct ccgcgtcgcg gatgagcgcc atggcggtct
     1981 tgatcttgat cgaaccgccc gggttgaagc cctccatctt caggtagatg tgatggcccg
     2041 gcgcgaagtc gtgcaaggtc aggaacgtgt cgtcgctgat gatgtgttcg atcgagtcgt
     2101 atttcatggc aggcgtctga cgggacgtgc gaaaaggggc atgcaggaag gtgacggccg
     2161 ggccgcttcg gctcagccga gcgccgcctg caggtcggcg gcgcgcagcg agtcgagcgg
     2221 ccacgagccg agcgccggcg gcgcgctgcg caaggtgtgg cgcgtcgact tgggcgggaa
     2281 gggacggccc gccagcccgc gcgcgatgac ctcgtcgggc gtgacgccga tgcctgggcg
     2341 gcgcgactcg agctccacgt cgcggtagtc cagcatcagg catggcacgc gctgcagcgc
     2401 ctggcgcagc gcgaactccc ggcggtgatg gccgtccatg acgaccaggc tcgagcgctc
     2461 gacgaggatc gggaacggcc actgtccgct cgccaggatc tcggctgtca gtgcgtcgag
     2521 ccggtcaggc gagaactctt ccgtcgggcg cagcaggccg ggcggcagca ggaggacttc
     2581 gggggatgtc gtgcgctcgt cgttcatcgg gatgtccgtc gggaggtggc cggcgtcggc
     2641 gggcagggtc gatcgggtca tgcgggggct ccgatcgcgg cgggcgaggc cggcgcgccg
     2701 gccgtttcca ggcgtgcgag cacgtcggcg agcggcaggt cctgcgaacg cacgcggcac
     2761 aactggccga cggccagcac gagccggtcg aggaggcggc gggcggtggg cgcgtcgaac
     2821 aggtcgcggc agtactgcag cgacagggtc acgccgtcgg tggactccgc gaccacgaag
     2881 cgcaggtcgt tgtaggtcgc gtcggtgccg caggccaccg gcacgagttc ccgtccgccc
     2941 aggcgcgggt tcgccccgcc ggtctgcacg tagctgaagt gcacctggaa gcgcgcggtg
     3001 tgcgaccggt cgggtcgcgg gccgcacagg tcgatgatcg cgctgcgcgg gatgtgctgg
     3061 ttcgacaggg cctcgaccac ccggtcgtgc acctgcgcga gcagggccgt tccgttggcg
     3121 tgcgggtcga gttgcccgcg gatcacgagg ccgcgggcat agaagccgac cgagccgaac
     3181 agctcggagg cgaagcggcc gccggcgggc gtgacgatga agaggtcgtc ctcgccggtc
     3241 tccacgcgca gcgtcgcgaa cagcgccgcc atcacctgcg tgtagcgcgt ccagccgtgg
     3301 cgggcgcctt gctcgcgcat gtcgcgcacg agctgggcat ccagcggcgc cgacagcccg
     3361 gcgccgcgat gcgtgaacac cgggggccgc ggatggtcgc acggcagcgg cagggtggtg
     3421 gccatgccgc gcatccgctc gcgccagtgc gcgacctgga tcgcggccag cggatcgtcg
     3481 cgcatgaacc ggcgttgcca gtcggcgaac gcgctgtact gcagcggcgg ctcgccgagc
     3541 agcgtcggcc attgcgagtc gggcgcgtcg tacaggcgcg tggcctcgtc ccagaagacg
     3601 tcgtcggatt cgccgtcgaa gacgaggtgg ctcacggcga tcgccagcac gatgtcgtcg
     3661 tcagccagcg tggccacgtg cactcgcatg agcggcgcct gcgcgatgtc gatcggcgcg
     3721 tggagcgcgg ccatgacgcg ggcctgcacg gcgtgcgggt cggccgcgat gccgcgcatg
     3781 tcctcgatga tgaacggcac gcgggcgttc tcgcgcacat gcacgcgcca gccgtccggg
     3841 ccgcggacga aggcgctgcg cagggcgtcg tggcgctgca gcaggtggtc gaacgcggcc
     3901 tgcagccgcg cgaggtcgag gcggcccgtg acgcgccagg cggcgcggat cccgtgcagt
     3961 tcgtcgcccg ggatcgcgtc gtccgcgagg tcgcgcagga tgtcctcctg cccgaagctc
     4021 gggcgccccg tcgcccaggc cgaccgtgcg ccggcgggca gcggcacgcc cgcggcttca
     4081 gcggcaccgg cggcgcggcg cgagcccagc cgcgcggcga gcgcggccac ggtgggggcc
     4141 tggaacacct cctgcagcga cagctcgaag gcgaagttcg cacgcaaggt ggcgaccagg
     4201 cgcaccgcga gcagcgaatg gccgccgagg ctgaagaagt tgtcgtgcac gccgacttgc
     4261 ggcaccttga gcaggtcggc ccacagcgag gccagcgagg cctcgagcgc gttgcgcggc
     4321 gcgacgggcg cggccggcgc accggcgccg tccgccgcga gcgccgccag ggcaacgcga
     4381 tcgacctgcc cgtccgcgcc gcgcggcagc cctgcgacga agtgccagcg catcggcagc
     4441 atcgcgggcg gcaactggcg cagcagatgt tcgcgcaggg cctcggcgct gacgctgcca
     4501 tcgtcctggg ccacgacgca ggcgaccagg cggtcctggc ccggcgcggg atcgaccagc
     4561 accgccactt ccgcgacgcc gggcgcagcc cgcaacgccg cctgcaccac cgccgtgtcg
     4621 agcaggcggc cctgcacggt cgacagcccg tcgacgcggc cgagccgctc gaggctgccg
     4681 tccggacggc agcgtccgcg ctcgccgctc gcgagcaggc gcgcgcccgg gcggccgaac
     4741 ggatcgggcc gccaggccgc
//
LOCUS       DATCRO010000649         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_97093, whole genome shotgun sequence.
ACCESSION   DATCRO010000649 DATCRO010000000
VERSION     DATCRO010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97093"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VIN75_17765"
     CDS             complement(<1..819)
                     /locus_tag="VIN75_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8976068.1"
                     /translation="MVANVGALVARSARANARRVAVMSPARTLTYAQLEQRSNRLANA
                     LLGLGLARGDRVGIYLANCAEIVEVEIACYKAGLVKAPFNSRLSPGEVGEIAANSEAA
                     IIVTTRERAESFRAHIRGEMPRLLLIDGDDATGYEAALARAGDAFTPVEVHADELAVL
                     HYTSGSSGVLKAAMQTFGNRLAQLRKFLIRSEGMHEGHILGLVGPVTHASGMQIVPAL
                     CSGVTIRLFSGFEPARFIADMRADRVTHTFMVPTMINMLLGELEGQYRPLPDLQR"
     gene            complement(1036..2049)
                     /locus_tag="VIN75_17770"
     CDS             complement(1036..2049)
                     /locus_tag="VIN75_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976069.1"
                     /translation="MDLRTLRYFIAVLEAGSLSRAAGALYVAQPALTAQIKKLESELG
                     VQLFERSHAGVTPTSAGLRLYDDARRLLSDADAMRERIQRLPQGPEGSVTLAIPFLLA
                     SLLMGPVLAALKESHPRVRVFVLDGLSLTVCKAVLERRADVGILVDTPPLEGLRCRPM
                     AREGMYFCGRDTDGRVARLLRAAPTVPAGRRRPARGDAGAAASQYPVIDLADAAAQPL
                     VLQSRRFAIRQSVEGAANARGVPLNIVHEHDSARVIRSLYLNGAGFTFVPACALSDSP
                     PPREGWLVARVVNPELSRAYTLVTPERPLDAVAQVVVDALTAEAKSLIGQRKWMAEWT
                     MRG"
     gene            2348..2716
                     /locus_tag="VIN75_17775"
     CDS             2348..2716
                     /locus_tag="VIN75_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HEY8976070.1"
                     /translation="MDTPPRSGGNAPQAHDSVAAWFDPLLSERLAQAGFGTLQQLAQR
                     INDAGVTWWYPVRGIGVRRAERVVQWLREQQESTGMQVDLRGVREHGRARPAPCDATP
                     TAAARGSDNARFVSAATEQG"
BASE COUNT          431 a          934 c          992 g          415 t
ORIGIN      
        1 gcgctgcagg tcgggcagcg ggcggtactg cccttcgagc tcgcccagca gcatgttgat
       61 catggtcggg accatgaagg tgtgcgtcac gcggtcggca cgcatgtcgg cgatgaagcg
      121 cgccggctcg aagcccgaga agaggcgaat cgtcacgccg ctgcacaacg ccggcacgat
      181 ctgcatgccc gaggcgtgcg tgaccggccc cacgaggccg aggatgtggc cctcgtgcat
      241 gccctcggag cggatcagga acttgcgcag ctgcgccagc cggttgccga aggtctgcat
      301 cgcggccttc agcacgcccg aggagcccga ggtgtagtgc agcacggcga gttcgtcggc
      361 atgcacttcc accggcgtga atgcatcgcc ggcgcgggcc agcgcggcct cgtagccggt
      421 ggcgtcgtcg ccatcgatca gcagcaggcg gggcatctcg ccgcggatgt gcgcacggaa
      481 gctctcggcg cgctcgcggg tggtgacgat gatcgcggcc tcgctgttgg cggcgatctc
      541 gcccacttcg ccgggcgaca ggcgcgagtt gaacggcgcc ttcacgaggc cggccttgta
      601 gcaggcgatc tcgacctcga cgatctcggc gcagttggcc aggtagatgc ctacgcggtc
      661 gccgcgcgcc aggccgaggc ccagcagcgc gttggcgagg cggttgctgc gctgctcgag
      721 ctgcgcgtag gtgagcgtgc gcgcggggct catcacagcg acgcggcgcg cgttggcgcg
      781 cgcgctgcgc gccacgaggg cgccgacgtt ggcgaccatg cgcctggggg gaatggatgg
      841 gggattgatg cttgtctcct gggtgccgcg acgtgcgatc gcgcatgtcg ccgttccgct
      901 cttgcggtca gcggcactgt tccagaagcg cggctgcagg agaaatactg tttgctgatc
      961 cggggccatc accggaattg atggccttcg gccgatggcg cgtgcccttg cgcgtgccct
     1021 tgttcgcgcc cgggctcagc cgcgcatcgt ccactccgcc atccacttgc gctgcccgat
     1081 gaggcttttc gcttcagcgg tcagcgcatc caccaccacc tgcgcgacgg catcgagcgg
     1141 acgctcgggc gtgaccagcg tgtaggcgcg cgaaagctcc gggttgacca cgcgagcgac
     1201 cagccagcct tcgcgaggcg gcgggctgtc gctcagcgcg caggcaggca cgaaggtgaa
     1261 gccggcgcca ttcagataga gggagcggat cacccgtgcc gagtcgtgct cgtggacgat
     1321 gttcagcggc acgccgcgcg cattcgccgc gccttccacc gactgccgga tcgcgaagcg
     1381 ccgcgactgc agcaccagcg gctgcgccgc ggcgtcggca agatcgatca ccgggtactg
     1441 cgatgccgcg gcgcctgcgt cacccctcgc cgggcgccgc cgccccgcgg gcaccgtggg
     1501 cgccgcgcgc agcagccggg cgacgcggcc atccgtatcg cgcccgcaga agtacatgcc
     1561 ttcgcgcgcc atcggcctgc agcgcaggcc ctccagcggc ggcgtgtcga ccaggatgcc
     1621 gacgtcggcg cggcgctcca gaacggcctt gcacaccgtc aggctcaggc cgtcgagcac
     1681 gaagacgcgc acgcgcggat gcgattcctt gagcgccgcg agcaccggcc ccatcagcag
     1741 cgaggcgagc aggaagggaa tggccagcgt gacggagccc tcgggaccct gcggcaggcg
     1801 ctggatgcgc tcgcgcatgg cgtcggcgtc gctcagcagg cggcgggcgt cgtcgtagag
     1861 gcgcagcccg gccgacgtgg gcgtgacgcc ggcatgcgaa cgctcgaaga gctgcacgcc
     1921 gagttcgctt tccagcttct tgatctgcgc cgtgagcgcc ggctgcgcca cgtacagcgc
     1981 cccggccgcg cgggagaggc tgccggcttc gagcacggcg atgaaatagc ggagggtgcg
     2041 caggtccatg ggttgcgagt gtggggccgg ggctggctac tatgcctcgc ggggtcgttc
     2101 tcgggcctcg caggcctccc gggcaggtaa accctgatcg gcgatcaggc atctgcccgg
     2161 aaactggaca cactatttgc ctgaataact tacatacgcc ggcagcggac caggaaatct
     2221 gttgtggatc aaggcgcagg cgcactggca tgtcccgtgc aacgcaagcc catccgatcc
     2281 acaacatgcg cggagtcatc gcgggccgtc aagcatcccg cactcgtgcc cgcgcctgga
     2341 gacaaccatg gacacccctc ctcgatccgg cggaaatgcg ccgcaggcac acgacagcgt
     2401 cgcggcgtgg ttcgaccccc tgctgtccga aaggctcgcg caggccggct tcggcacgct
     2461 gcaacagctg gcgcagcgca tcaacgatgc cggagtgacc tggtggtatc cggtgcgggg
     2521 catcggcgtg aggcgggccg agcgcgtcgt gcaatggctg cgcgagcagc aggagagcac
     2581 gggcatgcag gtggacctgc gcggcgttcg cgaacacgga cgcgcccgtc cggcgccctg
     2641 cgatgccacc ccgacggctg cggcgcgcgg ctcggataat gcgcgcttcg tgtcagcagc
     2701 aacagagcaa ggatgagcgc agcggatttt cttttttctc ccgaagtaca gaagctgctc
     2761 acggtcctgt ac
//
LOCUS       DATCRO010000650         7052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_851298, whole genome shotgun sequence.
ACCESSION   DATCRO010000650 DATCRO010000000
VERSION     DATCRO010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851298"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VIN75_17780"
     CDS             complement(<1..777)
                     /locus_tag="VIN75_17780"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8976071.1"
                     /translation="MSSFLPAASPGLEAEEPPRSRWGRFLRALTPRMIGLTLASSLVV
                     AIVMSPIFELPFTVLLGRTLFVGFVTLLARSAAVASSQRLVPPWVLQSVSVLCGAILA
                     TLAVYLLATGGDFSAVVQHEGRIQGFILISGTGTIVGLMLALGALERQRDSEAKALGL
                     ELALERSRLERHAADARLAVLTAQVEPHFLFNTLANVQALVETGSPRAPAVLRSLIAY
                     LKATMPRLDDGDARLGRETTLVRSYLELMHLRMPDRLQFTV"
     gene            complement(897..3755)
                     /locus_tag="VIN75_17785"
     CDS             complement(897..3755)
                     /locus_tag="VIN75_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8976072.1"
                     /translation="MPQNRPIPSLTVLASACLALLSACATAPEAAQPPGTITEVAAAE
                     PAQAASAATPASGASAAAAKAGAPARAAAPSGPPLPPFATVTQGARKVEGMLNLWQKE
                     DKVWLELRPEDFNKPMFFSPKISQGLGEGGVFGGTVMRAYSPWGAPQIVEFRKIHNMV
                     QLVALNETFRAKAGTPEARAVAAAYSSSLVGSAPVASQADASSHGVLVEANNLFLVDT
                     LGMAIHLQQMYRQGYGFDQRNSYFDGVRGTADEVMFDVTAHYFTQSIATSFPNAPANT
                     PQPTLPSFVPDARSLFLGLHYSLARLPDKPMATRPADPRVGYFTTTVADFTDDLARSP
                     RERFVNHWRLEKKDPAAALSEPVKPITYWLDRSIPVKYRAAITRGILEWNKAFERIGF
                     KDAIVVKVQPEDANFDTLDVGVTSVRWTVNADPQYGAIGPTHVDPRTGEILDAAISIE
                     SLSSRDLRNVRSQVLDPAGSASLSSLLQAPASELPPAAATVGRSAAAASADFCMAGPY
                     EAEQAGYGLDVLAARGEIDPDSPEAEAYVEAYLQDVTMHEVGHTLGLRHNFRASRMNS
                     EKDLDNPEFTKTHPLGGSVMDYLPINLPRPGEPAGKPFDSTLGPYDYWAIEYAYKPLP
                     PDATPAQQKAELLRIASRNTEPGLDYGTDEDNFLGVDPAALQGDLGADPVAYAKKRIA
                     IARDLFARQESRTLKPTEDYGVLRRSVTFALRDATRSAGVLLRQLGGVSTLRDFPGSG
                     RDPMEPVPGNVQRAALDTLVDGVLAADSFRVSPALQRRLAPDFLERGDTSAGGDPVPT
                     DYPVESVMLGLQRQVLAALMSDGLAQRLEDSAPKLDRAAQAQALTTAEVYARITRSLW
                     QDAANFGPDGKGDIPARRRELQRDHVNHLAAVLLRTPGGVRVDMRSTQRAEAVALLPR
                     IEAARRRPGLSEATRLHLADCAETLRVALAAPMQRAGY"
     gene            3910..4155
                     /locus_tag="VIN75_17790"
     CDS             3910..4155
                     /locus_tag="VIN75_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8976073.1"
                     /translation="MEATVAERGQITLPKAVRDALGLAKGTVLKVELDGGRIILRKDV
                     SDAISRVRGRFKLPDGVSADDVMQDLRGRKPGETPEF"
     gene            4202..4618
                     /locus_tag="VIN75_17795"
     CDS             4202..4618
                     /locus_tag="VIN75_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8976074.1"
                     /translation="MIAIDTSVLIDLLGDGEGADAAEACLRLALGSGPVVVCDVVASE
                     ITAGLGHGAEVMDALEDMGLGFSAVEQRSAVRAGEMQRKYMQRARAAGQSVGPRTMPD
                     FLVGAHALLQCNGLITRDEGFFRDYFKGLKVIVPKT"
     gene            4665..>7052
                     /gene="acnB"
                     /locus_tag="VIN75_17800"
     CDS             4665..>7052
                     /gene="acnB"
                     /locus_tag="VIN75_17800"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEY8976075.1"
                     /translation="MLEAYRQHVAERAALGIPPLPLNAQQTADVIELLKAPPAGEDAF
                     LLDLLTHRVPPGVDDAAKVKASFLSAVAHGDVEVALISKAKATELLGTMLGGYNVKPL
                     IDLLDHADVAQEAANALKKTLLMFDYFHDVAEKATAGNKFAAEVIDSWAKAEWFTSRP
                     EVPKKITVTVFKVTGETNTDDLSPAPDAWSRPDIPLHYLAMLKNARPGITPEEDGKRG
                     PMQFIEDLKKKGHLVAYVGDVVGTGSSRKSATNSVIWATGEDIPFVPNKRFGGVTLGG
                     KIAPIFFNTQEDSGALPIEVDVTAFNMGDVIDIFPYEGRIEKDGVKAVDFKLKSDVLF
                     DEVRAGGRINLIIGRSLTAKAREFLKLPASTAFRLPQSPAASTKGFSLAQKMVGRACG
                     LPEGQGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKMHHELPAFISNRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFGAATGVMPLDMPESILVRFKGQMQPGITLRDLVHAIPLYAIKKGLLTV
                     AKQGKVNAFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVHLNKEPIIEYMTSN
                     IVLMKNMIADGYQDKRTLERRIKAMEAWIADPQLLKADSDADYAEVIEIDLAEIKEPI
                     LCCPNDPDDAKFLSDVAGTRIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPP
                     TKMDASELTKEGHYGTFGTAGARMEMPGCSLCMGNQAQVREGATVVSTSTRNFPNR"
BASE COUNT         1106 a         2453 c         2503 g          990 t
ORIGIN      
        1 cacggtgaac tgcaggcggt cgggcatgcg caggtgcatc agctcgaggt agctgcgcac
       61 cagcgtcgtc tcgcggccca ggcgcgcgtc gccgtcgtcc aggcgcggca tcgtggcctt
      121 caggtaggcg atcaggctgc gcagcaccgc cggcgcgcgc ggcgagcccg tctcgaccag
      181 ggcctgcacg ttggccagcg tgttgaacag gaagtgcggc tcgacctgcg cggtgagcac
      241 ggccaggcgc gcgtcggcgg cgtggcgctc caggcgcgag cgctccagcg ccagctccag
      301 gcccagcgcc ttggcctcgg agtcgcgctg gcgctccagc gcgcccagcg ccagcatcag
      361 gccgacgatg gtgccggtgc cggaaatgag gatgaagccc tggatgcggc cttcgtgctg
      421 gacgaccgcg ctgaagtcgc cgcccgtggc cagcaggtag accgccagcg tggccaggat
      481 ggcgccgcac agcaccgaca ccgactgcag cacccacggc ggcaccaggc gctggctgga
      541 cgccaccgcg gcgctgcgcg ccagcaacgt gacgaagccc acgaacagcg tgcgccccag
      601 cagcacggtg aacggcagct cgaagatcgg gctcatcacg atcgcgacca ccagtgacga
      661 cgcgagcgtc agcccgatca tgcgcggcgt gagcgcccgc aggaagcggc cccagcgcga
      721 acgcggcggc tcctcggcct cgagaccggg cgacgcggcg ggaaggaaag acgacatgac
      781 ggcacgataa cgcgcgccgc cgcccccggc catgggcgat ggcccgcggt gcgacggacg
      841 acggatggcg ggggacgggc ggcgggccga gcgacggccc tgcgcgcccc gccctgtcag
      901 tagcctgcgc gctgcatggg agccgccagc gcgacgcgca gcgtctcggc gcaatcggcc
      961 aggtgcaggc gcgtggcctc gctcaggccc ggacggcgcc gcgccgcctc gatgcgcggc
     1021 agcagggcca cggcctctgc gcgttgggtg gaacgcatgt ccacccgcac tccacccggc
     1081 gtgcgcagca gcaccgcggc caggtggttg acgtggtcgc gctgcagctc gcgccggcgt
     1141 gccgggatgt cgcccttgcc gtcgggcccg aagttggccg cgtcctgcca gagcgagcgc
     1201 gtgatgcggg cgtagacctc ggccgtcgtg agggcctggg cctgcgcggc gcggtcgagc
     1261 ttgggcgcgc tgtcctccag gcgctgggcc aggccgtcgc tcatcagcgc ggcgagcacc
     1321 tggcgctgca ggcccagcat gaccgattcg accgggtagt cggtgggcac cgggtcgccg
     1381 ccggccgatg tgtcgccgcg ctcgaggaag tcgggcgcga ggcggcgctg cagggccggc
     1441 gagacgcgga agctgtcggc cgccagcacg ccgtcgacga gggtgtcgag cgcggcgcgc
     1501 tgcacgttgc cgggcaccgg ctccatcggg tcgcggcccg aaccggggaa atcgcgcagc
     1561 gtcgacacgc cgcccagctg gcgcagcagc acgccggccg agcgcgtggc gtcgcgcagc
     1621 gcgaacgtga cggagcggcg cagcacgccg tagtcctcgg tgggcttgag cgtgcgcgac
     1681 tcctggcgcg cgaacaggtc gcgcgcgatg gcgatgcgct tcttcgcgta ggccaccggg
     1741 tccgcgccga ggtcgccctg cagggctgcc ggatcgacgc cgaggaagtt gtcctcgtcg
     1801 gtgccgtagt ccaggccggg ctcggtgttg cgcgacgcga tcctgagcag ctcggccttc
     1861 tgctgcgcgg gcgtggcgtc gggcggcagc ggcttgtacg cgtactcgat ggcccagtag
     1921 tcgtacgggc cgagcgtcga atcgaagggc ttgcccgccg gctcgccggg gcgcggcagg
     1981 ttgatcggca ggtagtccat caccgagccg cccagcgggt gcgtcttcgt gaactcgggg
     2041 ttgtccaggt ccttctcgct gttcatgcgc gacgcgcgga agttgtggcg caggcccagc
     2101 gtgtggccga cctcgtgcat cgtgacgtcc tgcaggtagg cctcgacgta ggcctcggcc
     2161 tcggggctgt cggggtcgat ctcgccgcgc gcggccagca cgtccagccc gtagccggcc
     2221 tgctcggcct cgtacggacc ggccatgcag aagtcggcgg acgcggccgc ggcgctgcgg
     2281 cccaccgtgg ccgcggccgg cggcagctcg gaggccggcg cctgcagcag cgacgacagc
     2341 gaggcggagc ccgcggggtc gagcacctgc gagcgcacgt tgcgcaggtc gcgcgaggac
     2401 aggctctcga tggagatggc cgcatcgagg atctcgcccg tgcgcgggtc gacgtgcgtc
     2461 gggccgatgg cgccgtattg cggatcggcg ttgaccgtcc agcgcaccga ggtgacgccc
     2521 acgtccagcg tgtcgaaatt cgcatcctcg ggctgcacct tcacgacgat ggcgtccttg
     2581 aagccgatgc gctcgaaggc cttgttccat tcgaggatgc cgcgcgtgat ggcggcgcgg
     2641 tacttgacgg ggatgctgcg gtccagccag tacgtgatgg gcttgaccgg ctcggacagc
     2701 gccgcggccg gatccttctt ctccaggcgc cagtggttga cgaagcgctc gcgcggggaa
     2761 cgggcgaggt cgtcggtgaa gtcggccacc gtggtggtga agtagcccac gcgcgggtcg
     2821 gccgggcgcg tggccatggg cttgtcgggc aggcgcgcca gcgagtagtg caggcccagg
     2881 aacaggctgc gcgcgtcggg cacgaagctg ggcagcgtgg gttgcggcgt gttggccggc
     2941 gcgttgggga acgacgtggc gatcgactgc gtgaagtagt gcgccgtgac gtcgaacatc
     3001 acctcgtcgg ccgtgccgcg cacgccgtcg aagtacgagt tgcgctggtc gaacccgtag
     3061 ccctggcggt acatctgctg caggtggatg gccatgccca gcgtgtcgac caggaacagg
     3121 ttgttggcct cgaccagcac gccgtgcgag ctcgcgtcgg cctggctggc caccggcgcg
     3181 ctgccgacca ggctgctgga gtacgcggcc gcgaccgcgc gcgcctcggg cgtgccggcc
     3241 ttggcgcgga aggtctcgtt gagcgcgacc agctgcacca tgttgtggat cttgcggaac
     3301 tcgacgatct gcggcgcgcc ccagggcgag tacgcgcgca tcaccgtgcc gccgaacacc
     3361 ccgccctcgc ccaggccctg cgagatcttg ggcgagaaga acatcggctt gttgaagtcc
     3421 tcgggccgca gttccagcca gaccttgtcc tccttctgcc agaggttgag catgccctcg
     3481 accttgcgcg ccccctgcgt cacggtggcg aacggcggca gcggcggccc ggacggcgcc
     3541 gcggcgcgcg ccggggcgcc ggccttggcg gccgccgcgc tcgcgccgct ggcgggcgtg
     3601 gccgccgagg cggcctgcgc gggctcggcc gccgccacct ccgtgatggt gccgggcggc
     3661 tgcgccgcct cgggggcggt cgcgcaggcg ctcagcagcg caaggcaggc ggacgccagc
     3721 acggtcaacg acgggatcgg gcggttctga ggcatggaat caggagtcgt gagggggcga
     3781 taggttccgg gcgcgggcaa aagtacggca aaaaagtcag tgattaccct gtacgttcga
     3841 ttcggtacgt atagaatatg cataccgaaa ggttccgcat cgtactttca aaacgcaagg
     3901 tcgaacacca tggaagccac cgtcgccgaa cgcggccaga tcacgttgcc caaggccgtg
     3961 cgcgatgcgc tcgggctggc gaagggcacc gtgctgaagg tcgagctgga tggcggcagg
     4021 atcatcctgc gcaaggatgt cagcgacgcg atctcgcgcg tgcgcggccg gttcaagctg
     4081 cccgacggcg tgtccgccga cgacgtgatg caggacctgc gcggccgcaa gccgggcgag
     4141 acgccggaat tctaatgacg cgacaccccg cgctgctgtc ccggcgctgt cacttcgaac
     4201 catgatcgcc atcgacacct ccgtgctgat cgacctgctg ggcgacggcg aaggcgccga
     4261 cgccgccgag gcctgcctgc ggctggcgct cggcagcggt ccggtggtgg tgtgcgacgt
     4321 cgtcgccagc gagatcaccg ccggcctcgg ccatggcgcc gaggtgatgg acgcgctcga
     4381 ggacatgggc ctgggcttct cggccgtcga gcagcgctcc gcagtgcgcg ccggcgagat
     4441 gcagcgcaag tacatgcagc gcgcgcgcgc cgccggccag tccgtcggcc cgcgcacgat
     4501 gcccgacttc ctcgtgggag cgcacgcgct cctgcagtgc aacggcctca tcacccgcga
     4561 tgaaggcttc ttccgcgact acttcaaggg cctcaaggtc atcgtgccga agacttgagg
     4621 ccgcttcgtt ttcaccccag acgcttcttt tccaggagaa ctccatgctc gaagcctacc
     4681 gccaacacgt ggccgaacgt gccgccctcg gcatcccgcc gctgccgctg aacgcccagc
     4741 agacggccga cgtcatcgag ctgctgaagg cgccgccggc cggcgaggac gcgttcctgc
     4801 tcgacctgct gacgcaccgc gtgcccccgg gcgtggacga cgcggccaag gtcaaggcca
     4861 gcttcctgtc ggccgtcgcg cacggcgacg tcgaggtggc cctgatctcc aaggccaagg
     4921 ccaccgaact gctcggcacg atgctgggcg gctacaacgt caagccgctg atcgacctgc
     4981 tggatcacgc cgacgtcgcc caggaagccg cgaacgcgct gaagaagact ctgctgatgt
     5041 tcgactactt ccacgacgtc gccgagaagg ccacggccgg caacaagttc gccgcggagg
     5101 tgatcgacag ctgggccaag gccgagtggt tcacgtcgcg tccggaagtg cccaagaaga
     5161 tcaccgtcac cgtcttcaag gtcaccggcg agaccaacac cgacgacctg tcgcccgccc
     5221 ccgacgcatg gagccgcccc gacatcccgc tgcactacct ggcgatgctc aagaacgcgc
     5281 gccccggcat cacgcccgag gaagacggca agcgcggccc gatgcagttc atcgaggacc
     5341 tgaagaagaa gggccacctc gtggcctacg tcggcgacgt ggtgggcacc ggctccagcc
     5401 gcaagagcgc caccaacagc gtgatctggg ccacgggcga ggacatcccc ttcgtgccga
     5461 acaagcgctt cggcggcgtc acgctcggcg gcaagatcgc cccgatcttc ttcaacacgc
     5521 aggaagactc cggcgcgctg ccgatcgagg tcgacgtgac cgccttcaac atgggcgacg
     5581 tcatcgacat cttcccgtac gagggccgca tcgagaagga cggcgtcaag gccgtcgact
     5641 tcaagctcaa gtccgacgtg ctgttcgacg aggtgcgtgc cggcggacgc atcaacctga
     5701 tcatcggccg ctcgctcacc gccaaggcgc gcgagttcct gaagctgccc gcctccactg
     5761 cgttccgcct gccgcaatcg ccggccgcct cgaccaaggg cttctcgctg gcgcagaaga
     5821 tggtcggccg cgcctgcggc ctgcccgaag gccagggcgt gcgcccgggc acgtactgcg
     5881 agccgaagat gaccaccgtc ggcagccagg acaccaccgg cccgatgacg cgcgacgagc
     5941 tcaaggacct cgcctgcctc ggcttctcgg ccgacatggt gatgcagtcg ttctgccaca
     6001 ctgcggcgta ccccaagccg gtcgacgtca agatgcacca cgagctgccc gcgttcatct
     6061 cgaaccgcgg cggcgtcgcg ctgcgtccgg gcgacggcgt gatccactcg tggctcaacc
     6121 gcctgctgct gcccgacacc gtgggcaccg gcggcgacag ccacacccgc ttcccgatcg
     6181 gcatcagctt cccggccggc tccggcctgg tcgccttcgg cgccgccacc ggcgtgatgc
     6241 cgctggacat gcccgaatcg atcctcgtgc gcttcaaggg ccagatgcag ccgggcatca
     6301 cgctgcgcga cctcgtgcac gcgatcccgc tgtacgcgat caagaagggc ctgctgaccg
     6361 tggccaagca gggcaaggtc aacgcgttct cgggccgcat cctcgagatc gagggcctgc
     6421 ccgacctgaa ggtggaacag gcgttcgagc tgtccgacgc gtcggccgaa cgttcggccg
     6481 ccggctgcac ggtgcacctg aacaaggagc cgatcatcga gtacatgacc tcgaacatcg
     6541 tgctgatgaa gaacatgatc gccgacggct accaggacaa gcgcacgctg gagcgccgca
     6601 tcaaggcgat ggaggcctgg atcgccgacc cgcagctgct caaggccgac tccgatgccg
     6661 actacgccga ggtcatcgag atcgacctgg ccgagatcaa ggaaccgatc ctgtgctgcc
     6721 cgaacgaccc ggacgacgcc aagttcctgt ccgacgtggc cggcaccagg atcgacgagg
     6781 tgttcatcgg ctcgtgcatg accaacatcg gccacttccg cgcggcgtcc aagctgctcg
     6841 aaggcaagcg cgacatcccg gtcaagctgt gggtcgcccc gccgaccaag atggacgcct
     6901 ccgagctgac caaggaaggc cactacggca ccttcggcac ggccggcgcg cgcatggaga
     6961 tgccgggctg ctcgctgtgc atgggcaacc aggcgcaggt gcgcgagggc gcgacggtcg
     7021 tctccacgtc gacccgcaac ttcccgaacc gc
//
LOCUS       DATCRO010000651         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_929756, whole genome shotgun sequence.
ACCESSION   DATCRO010000651 DATCRO010000000
VERSION     DATCRO010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929756"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VIN75_17805"
     CDS             <1..545
                     /locus_tag="VIN75_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8976076.1"
                     /translation="RVLFLDEPTAGVDVELRHDMWQMVRELRENGVTIILTTHYIEEA
                     EEMADRIGVIAKGRLLLVEDKTELMRKLGRKQVTLMLPQALAEVPAALHDYELALSPD
                     GHQLTYTYDTRNESRGIVDFIRHVNDAGVQYTDLQTKQSSLEEIFVSLVEGERDRPAR
                     PPEGRMPPLGGQARSAGGAR"
     gene            542..1312
                     /locus_tag="VIN75_17810"
     CDS             542..1312
                     /locus_tag="VIN75_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976077.1"
                     /translation="MNAANWHAVRAIYRFEMARTWRTIMQSIISPVISTSLYFVVFGA
                     AIGSRIQTVGSIPYGAFIVPGLIMLSLLTQSISNASFGIYFPKFTGTIYELLSAPVSW
                     LEITIAYVGAAASKSIILGLIILATAGLFVPLRVEHPIVMALFLVLTAVTFSLVGFII
                     GIWADGFEKLQVIPLLIVTPLTFLGGSFYSISMLPPFWQTVALFNPVVYLISGFRWSF
                     YGTGDVSLGLSLAMTLAFLAVSMGIVAWIFRTGYRLKN"
     gene            complement(1378..1569)
                     /locus_tag="VIN75_17815"
     CDS             complement(1378..1569)
                     /locus_tag="VIN75_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976078.1"
                     /translation="MTKSLVQAVAFALSALTTLGTVAAANGIAARQFVAATHEAEAAD
                     GTTHLAMQRVVIVAHRAKA"
     gene            complement(1993..2211)
                     /locus_tag="VIN75_17820"
     CDS             complement(1993..2211)
                     /locus_tag="VIN75_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976079.1"
                     /translation="MLKTSARATALVLSVFLTTGTVAAANGLATQQYAAAGRNAQEYE
                     LTHMAMQRVEIVAPGAFQRVVVIGRRAA"
     gene            2511..2786
                     /locus_tag="VIN75_17825"
     CDS             2511..2786
                     /locus_tag="VIN75_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976080.1"
                     /translation="MDIKQDPNAIPNFNAGGRIGEPQRNEPEQVQSSVELDPTAGGDG
                     ATSEAGEHESLLGDGSGESVRPDGPSGGREDPSKSGPGAVDGLVGAP"
BASE COUNT          432 a          937 c         1027 g          460 t
ORIGIN      
        1 cgcgcgtgct gttcctcgac gagccgaccg cgggcgtcga cgtcgagctc cgtcacgaca
       61 tgtggcagat ggtgcgcgag ctccgcgaga acggcgtcac gatcatcctg acgacgcatt
      121 acatcgagga ggccgaggag atggccgatc gcatcggcgt catcgccaag ggcaggctgc
      181 tgctggtcga ggacaagacc gagctgatgc gcaagctggg caggaagcag gtgacgctga
      241 tgctgccgca ggcgctcgcc gaagtgcccg cggcgctgca cgactacgag ctcgcgctgt
      301 cgcccgacgg ccaccagctg acctacacgt acgacacccg caacgagtcg cgcggcatcg
      361 tcgacttcat ccgccacgtc aacgacgcgg gcgtgcagta cacggacctg cagaccaagc
      421 agagttcgct cgaggagatc ttcgtgagcc tggtcgaggg agaacgcgat cggcccgcgc
      481 gtccgcccga agggcgaatg ccgccgctcg gcgggcaggc gcgcagcgcc gggggtgcgc
      541 gatgaacgcc gccaactggc acgcggtgcg tgcgatctac cgcttcgaga tggcgcgcac
      601 ctggcgcacg atcatgcaga gcatcatctc gccggtcatc tcgacctcgc tgtacttcgt
      661 cgtgttcggc gcggcgatcg gctcgcgcat ccagacggtg ggcagcatcc cctacggcgc
      721 gttcatcgtg ccggggctga tcatgctgtc gctgctgacg cagagcatct ccaacgcgtc
      781 gttcggcatc tacttcccga agttcaccgg cacgatctac gagctgctgt cggcgcccgt
      841 gtcgtggctg gagatcacca tcgcctacgt gggcgcggcg gcgtccaagt ccatcatcct
      901 cgggttgatc atcctggcca cggccggcct gttcgtgccg ctgcgcgtcg agcacccgat
      961 cgtgatggcg ctgttcctgg tgctgacggc ggtcacgttc agcctggtgg gcttcatcat
     1021 cggcatctgg gccgacggct tcgagaagct gcaggtgatc ccgctgctga tcgtcacgcc
     1081 gctgaccttc ctgggcggca gcttctattc gatctcgatg ctgccgccgt tctggcagac
     1141 cgtggcgctg ttcaacccgg tggtctacct gatttcgggc tttcgctggt cgttctacgg
     1201 caccggcgac gtcagcctgg ggctgagcct ggcgatgacg ctggcgttcc tggcggtctc
     1261 gatggggatc gtcgcgtgga tcttcaggac gggttatcgg ttgaagaact gatcgccggg
     1321 aggcctgcca cgcaatggcc acgcgatggg cgtggccccg aaggagcgag cggggcgtca
     1381 ggccttggcg cgatgcgcga cgatcacgac ccgctgcatg gccaggtgcg tcgtgccatc
     1441 ggcggcctcg gcctcgtgcg tcgcggccac gaactgcctg gcggcgatgc cgttggcggc
     1501 ggcgacggtg cccagcgtgg tgagggcgga cagggcgaat gcgacggctt ggacgaggct
     1561 cttggtcatg atgggctcct gggtgtctgg ggggctgtcc ggaacgcgtt tcgtgttccg
     1621 atggatggac tgtgttcctc ccgaaccagg cttcccagcg gcttgcgacg attggggccg
     1681 gagctggaac cagctgcgat tgcgcgcgat gaaccggcgg gcgataccgc gagctgtatg
     1741 gacgccggcg cgggaagctg ctggcgaagg ggttggcaac agcaggaggg gcctggcgtt
     1801 gccgccatcc gcgcccgatg gtctttccag tcacccgtca agccgcgcgg acgcaggaga
     1861 tgccaggccc ctttccgctg ttgccagacg ccgctggcgg caaccatccc ggtgttgcca
     1921 aaaagcaaaa ggcctcgcgc acggcgaggc cttggtgggg aacggatgcg cgccggctgg
     1981 cgcgctttcg ggtcaggcgg cgcggcggcc gatgaccacg acgcgctgga atgcgcccgg
     2041 ggcgacgatc tcgacgcgtt gcatggccat gtgcgtcagc tcgtattcct gcgcgttgcg
     2101 acccgcggcc gcgtactgct gggtcgcgag gccgttcgcc gcggcgacgg tgccggtggt
     2161 gaggaagacg gacagcacga gggcggtggc gcgggcggag gtcttgagca tggcaatctc
     2221 ctggggtggg tcggaacgcg tgggtgttcc gatgcctgca ctgtgatccg atcggggccc
     2281 gacgggaagc cccttgcgac gatcgacgcc ggcgccggcg tgaaatgctt cctggtgcga
     2341 cgaaccgatt cgtgcatcgg cgaaccggct ttcgcccggg ccggcgccgg cccgcctcgg
     2401 caggcgccgt caagaccccg cccgccacgc gggcaccgag ggaacaccgg ttgcctcgac
     2461 agcgcatcgc ggcccgcgcg ccgccccgcg ccccgtgcga gaggactccc atggacatca
     2521 agcaagaccc caacgcgatc ccgaatttca acgccggcgg ccgcatcggc gagccgcagc
     2581 gcaacgagcc ggaacaggtg caatccagcg tcgagctgga ccccaccgcg ggcggcgacg
     2641 gcgcgaccag cgaggccggc gagcatgaat ccctgctggg cgacggcagc ggcgagtccg
     2701 tccggcccga cggaccttcc ggcgggcggg aggacccgtc caaatccggc ccgggcgccg
     2761 tcgacggact ggtcggcgcg ccctaggagt ctgcgcggca gaaggcgcct gctgcgaacg
     2821 gcgataagcg gggccttttc agcggcggct cctctc
//
LOCUS       DATCRO010000652         9086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_547633, whole genome shotgun sequence.
ACCESSION   DATCRO010000652 DATCRO010000000
VERSION     DATCRO010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547633"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VIN75_17830"
     CDS             complement(<1..145)
                     /locus_tag="VIN75_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976081.1"
                     /translation="MRFDLVTLNLVLAIAETRSITRGAEREHLALAAASKRLSDLEAR
                     LGVG"
     gene            complement(794..985)
                     /locus_tag="VIN75_17835"
     CDS             complement(794..985)
                     /locus_tag="VIN75_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976082.1"
                     /translation="MTKTTAHAAAAVLSVLMSFGIVAGMNGIATRQYSAAERVAQVAA
                     NATQVAAVQRVVVTARRTA"
     gene            complement(1223..2446)
                     /locus_tag="VIN75_17840"
     CDS             complement(1223..2446)
                     /locus_tag="VIN75_17840"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY8976083.1"
                     /translation="MEAIQSLVDAFVGFFSTGVAHATWWELVIYTLVMTHITIAAVTI
                     FLHRCQAHRALDLGPIPSHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETVDDPHSPVT
                     RGIKTVLLRGAELYRAESKNQETIKKFGHGTPDDWIERNVYSRFQWQGVAIMMIINLV
                     LFGAIGATIWAVQMLWIPITAAGIINGIGHYWGYRNFEAVDASRNILPWGIIIGGEEL
                     HNNHHTYPTSAKLSVKPFEFDIAWGYIRAMEIVGWAKVRKVAPQMKTGDVRAVADSET
                     LEAIIANRYEVMAKYARELRRACAAEVAALKSKGEQSAALANMRVARRWLHRDVEKIP
                     GHLQAQVADAAAASEKLGKLVAMREELRALWTRTNVSAEQAVADLQAWCKQAEESGIE
                     ALREFSFKLRAAKAA"
     gene            complement(2644..3903)
                     /locus_tag="VIN75_17845"
     CDS             complement(2644..3903)
                     /locus_tag="VIN75_17845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY8976084.1"
                     /translation="MHPNALLETATELLREVGKLDMPADVIVSNFFRKHRELGPRERH
                     ALAETAYAVLRQRLLYQHLAQSGSGTLERRLAILAWQGNESQIRGALGPHEQQWLNQV
                     KAIDRSAFSEKLRHNMPDWLAGALKNELDDAGFWNFVGAMSGSAPLDLRVNMLKSSRD
                     KAQAQLREAEIESMPTPYSPWGLRVQGKPALQKSDVFLRGDIEVQDEGSQLLAALVAP
                     KRGEMVVDFCAGAGGKTLALGAAMRSTGRLYAFDVNGHRLDKLKPRLARSGLSNVHPA
                     QLTNERDDRVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRLSSKAIDELVELQGRIL
                     ESASRLLKSGGRLVYATCSPLRREDEAIAEAFSAAHPEFRLLDAGELLKAAGVERAEE
                     LNAGGYMRSWPHAHSMDGFFAAAWQKA"
     gene            complement(4017..4658)
                     /gene="purN"
                     /locus_tag="VIN75_17850"
     CDS             complement(4017..4658)
                     /gene="purN"
                     /locus_tag="VIN75_17850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY8976085.1"
                     /translation="MKRIAVLISGQGSNLGAIVRASQAQDWPGRVVAVISNRADARGL
                     QFAQNHGIATAVLDHKAYIDRPAFDAALAETIDGFAPDLVVLAGFMRILTPQFVARYE
                     GRMLNVHPSLLPSFSGLHTHRRALEEGCKVHGATVHFVSAELDHGPIVAQAVVPVLAG
                     DDEATLSARVLAAEHVLYPRAVRWFLEGALELKDGRVTHRGHEPQGWLAEPAA"
     gene            4757..5956
                     /locus_tag="VIN75_17855"
     CDS             4757..5956
                     /locus_tag="VIN75_17855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8976086.1"
                     /translation="MRARVLSGMRPTGALHLGHYHGALKNWVRLQHEYDSFFFVADWH
                     ALTTHYQDRDVIERNVYDMVIDWIAAGLDPDLCTIFIQSRLPEHAELFTLLAMGTPLG
                     WLERVPTYKDQIEHLSDRDLATYGFLGYPLLQAADILIYKAAYVPVGEDQDSHVELTR
                     EVARRFNHLYGRSPRFEAQVAAALAKLPPEQRDLLERQRRQFGQDGDVAALGNGEALV
                     RAHAGHLSSEDAELLLGHLKGSGRSVLHEPQALHTEVTKLPGLDGQKMSKSYGNAIEM
                     REEPAQVEAKIRRMPTDPQRVRRTDAGDPARCPVWQFHKVYSDAATQQWAHDGCTTAG
                     IGCLDCKQPVIDAILREQRPWRERAQQYLANPKQVHWIVEMGTERARTVARQTMKDVR
                     DVMGLSY"
     gene            5992..6456
                     /locus_tag="VIN75_17860"
     CDS             5992..6456
                     /locus_tag="VIN75_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEY8976087.1"
                     /translation="MAGIHITDIESAINWWRAQFPSPDGITACPQVLALAEVYALMVY
                     YREHEADEFTLPAVAREAWLEWYASTPDAPCIAICSTSQGDHECKGCGRTFDEVQHWT
                     AMSPGQKRAVWRRITLDAKAWRFNKYAERAHKATGEDHPDPAQLDAAAPARS"
     gene            complement(6494..7903)
                     /locus_tag="VIN75_17865"
     CDS             complement(6494..7903)
                     /locus_tag="VIN75_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8976088.1"
                     /translation="MRSLYLRIWLTVIVTLALFAGASGWLVQRHLDQEHARNQAALTE
                     RIAAWGDLIQRSLPAAAAPPDVQAEALRDWSQRLRVPMALEDEQGHRIAASDSFVRRE
                     ADKALMHVAIYQIKLDDGRSLYVMRPNMLKPPGGNDPAMPPDEDIGNFGTEHHPQATE
                     RSGPPAFLFPGVVPRGVSPGLSLLALLVVLFMAIAIGAYPFVRRLTRRLETLKRGVEA
                     FGQGKLDRRVEVEGRDEVATLASSFNQAAERIEALVRSNKSLLANASHELRSPLARLK
                     MATSLMSDPAVSEREREHLRAEIGTDIAELDGLVDEVLLASRLEAASEPTNLESVDLL
                     ALAVEEGARVDASVEGAPACVKGEERLLRRALRNLLENARRYGGGQIRVDLEDKAAAG
                     IEIRVSDNGPGVPDAFRERIFEPFFRLPGHAERAGGVGLGLALVRQIVERHHGRIRCV
                     RPPEGGGCFVISLPASIAA"
     gene            complement(8080..8784)
                     /locus_tag="VIN75_17870"
     CDS             complement(8080..8784)
                     /locus_tag="VIN75_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976089.1"
                     /translation="MTNPHLLLIDDDARLTRMVGDYLCTQGFDVDTAGTLADGRMRLA
                     AGSYDALVLDLMLPDGDGLDFTRELRGQPATRRLPLMMLTARGEPTDRIVGLELGADD
                     YLPKPFEPRELLARVKALLRRASPEPVNEEVLRFGKLEIDLGAHEARLDGVRCDLTSH
                     QFDLLVVLARSPGRVLSRDQIMDALKGHPLEAFDRSIDVHISRIRSVIEEDPKNPKRV
                     LTVRGSGYLFARKQDA"
BASE COUNT         1375 a         3091 c         3258 g         1362 t
ORIGIN      
        1 ggccgacacc gaggcgcgcc tcgaggtcgg acagccgctt gctggcggcc gccagcgcca
       61 ggtgctcgcg ctcggccccc cgcgtgatgc tgcgtgtctc ggcgatggcg agaacgagat
      121 tgagcgtgac gagatcgaag cgcatgggac cggactctgg ctgactggtg ggactgcaaa
      181 ggagtcagct gtgactgacg cggaacggcc cggtgcgcgg gcctcacact ggtaagcgtg
      241 gacccgctac cggtccggag acgccggata ggtgctccga tcgccgaacc gctgtcgcaa
      301 tgatgcgcga agggtcggca aagcgcgcgc ggctgtgatg aattcagcct tcttcgcaaa
      361 cgaagtctta tggtactgcg aagggatcgt cgtgtcgagg atccgggggt cgtttcccag
      421 gtctgactgt cgtgtggtcc aggcgcgcca aggcgcatgc gaagacggtg tccgccaatt
      481 cgcggggagt gtggctcccg ggcggtttgc tgaaggccgc atcgaggcaa agggtgtctg
      541 ccccaacacg acgtcatcct gcgcgcagtc gcaggatcca cgccggccgc gcgacggtct
      601 ggccatcctc gcggtgccta ggtggatcct gcgactgcgc gcaggatgac atgcctgttc
      661 ttcgttggag cggtgtctca ccccgaccag atccccgcag atcggcgatg aacaaaaaaa
      721 gcctcgcacc aacgagtgcg aggccgtgaa cggagcaaga ggcgccgcga agggcgcgcc
      781 aggctctgga ggttcaggcg gtgcggcggg ccgtcacgac cacgcgctgc acggcggcga
      841 cctgcgtcgc gttcgcggcg acctgcgcca cgcgctcggc ggccgagtac tgccgggtcg
      901 cgatgccgtt catgccggcg acgatgccga aggacatgag gacggacagg acggcggcgg
      961 cggcatgggc ggtggtcttg gtcatgatgg gctcccgggt gtctggttgg ttccggatcg
     1021 cgttgcgtga tccgatggat gaactatcgg agcaaccgga acgaccgccc agcggcttgc
     1081 gacgagtcga ccggaactcc gcaccaaccg cggatttcgc cgacgaaccg gagcccgcgc
     1141 cgcccgttcg acgcgccgct ccccggccca ggaacgacaa agcccgccga agcgggcctt
     1201 ggaacgacga gagcgtcgag gctcaggcgg ccttggcggc gcgcagcttg aacgagaact
     1261 cgcgcagtgc ctcgatgccc gattcctcgg cctgcttgca ccaggcctgc aggtcggcga
     1321 cggcctgttc ggcggacacg ttcgtgcgcg tccacagcgc gcgcagttcc tcgcgcatcg
     1381 ccaccagctt gccgagcttc tcggacgcgg cggcggcgtc ggcgacctgg gcttgcaggt
     1441 gtccggggat cttctcgacg tcgcggtgca gccagcggcg cgcgacgcgc atgttggcca
     1501 gcgcggccga ctgctcgccc ttgctcttga gcgcggccac ttcggcggcg caggcgcggc
     1561 gcagttcgcg ggcgtacttg gccatcactt cgtagcggtt ggcgatgatg gcttccagcg
     1621 tctcgctgtc ggccacggca cggacgtcgc ccgtcttcat ctgcggcgcg accttgcgca
     1681 ccttggccca gcccacgatc tccatggcgc ggatgtagcc ccacgcgatg tcgaactcga
     1741 acggcttgac cgacagcttg gccgacgtcg ggtacgtgtg gtggttgttg tgcagttcct
     1801 cgccgccgat gatgatgccc cacggcagga tgttgcgcga cgcgtcgacg gcttcgaagt
     1861 tgcggtagcc ccagtagtgg ccgatgccgt tgatgatgcc ggcggcggtg atggggatcc
     1921 acagcatctg gacggcccag atggtggcgc cgatggcgcc gaacagcacg aggttgatga
     1981 tcatcatgat cgccacgccc tgccactgga accggctgta gacgttgcgc tcgatccagt
     2041 cgtccggcgt gccgtggccg aacttcttga tggtttcctg gttcttcgat tcggcgcggt
     2101 agagctcggc gccgcgcagc agcaccgtct tgatgccacg ggtgaccggg ctgtgcgggt
     2161 cgtcgaccgt ctcgcacttg gcgtggtgct tgcggtggat ggcggcccat tccttggtga
     2221 ccatgccggt ggtcagccac agccagaaac ggaagaagtg ggacgggatc gggcccaggt
     2281 cgagggcgcg gtgcgcctgg cagcggtgca ggaagatcgt cacggccgcg atggtgatgt
     2341 gcgtcatcac cagcgtgtag atgaccagtt cccaccacgt ggcgtgcgcc accccggtcg
     2401 agaagaagcc cacgaacgcg tcgacgagag actgaatcgc ttccattgcc tgcctgtagg
     2461 tgccccgaaa tgaagggcgg tgttcaattg tagggggacg tccccaggga cagccaccaa
     2521 aattgcgcta ccgaccgggt tctttcctgg tttgagcgcc ggattttgat gcggatcaac
     2581 cctcgaagtt ctgctcgaca ggggcttggt cgtgcagaaa ttcggaatag ttgagaattc
     2641 ccctcaggct ttctgccagg ccgccgcgaa gaacccatcc atcgaatggg cgtgcggcca
     2701 gctgcgcatg tagccgccgg cgttcagctc ctcggcgcgc tccacgccgg ccgccttcag
     2761 cagttcgccg gcgtccagca gccggaactc cgggtgcgcg gccgagaacg cctcggcgat
     2821 cgcctcgtcc tcgcggcgca gcgggctgca ggtggcgtag accaggcggc cgcccgactt
     2881 gagcaggcgg ctggcgctct ccaggatcct gccctgcaac tcgaccagct cgtcgatggc
     2941 cttggacgac aggcgccact tcaggtcggg gttgcggcgc agggtgccca ggcccgagca
     3001 cggcgcgtcg acgagcacgc ggtcgatctt gccggccagg cgcttgacgc ggtcgtcgcg
     3061 ctcgttggtc agctgcgccg ggtgcacgtt ggacaggccg ctgcgcgcca ggcgcggctt
     3121 gagcttgtcc aggcggtggc cgttgacgtc gaacgcgtac aagcggccgg tggagcgcat
     3181 cgccgcaccg agcgccagcg tcttgccgcc ggcgccggcg cagaagtcga ccaccatctc
     3241 gccgcgcttg ggcgcgacca gcgcggccag cagctggctg ccctcgtcct gcacctcgat
     3301 gtcgccgcgc aggaagacgt ctgacttctg cagcgcgggc ttgccctgca cgcgcaggcc
     3361 ccacggcgaa tacggcgtgg gcatcgactc gatctcggcc tcgcgcagct gcgcctgggc
     3421 cttgtcgcgg ctggacttga gcatgttcac gcgcaggtcc agcggcgcgc tgcccgacat
     3481 ggcgcccacg aaattccaga agcccgcgtc gtcgagctcg ttcttgagcg cgccggccag
     3541 ccagtcgggc atgttgtggc gcagcttctc ggagaacgcg ctgcggtcga tcgccttgac
     3601 ctggttgagc cactgctgct cgtgcgggcc gagggcgccg cggatctgcg actcgttgcc
     3661 ctgccaggcc aggatcgcca gccggcgctc gagcgtgccg ctgccgctct gcgcgaggtg
     3721 ctggtagagc aggcgctggc gcagcacggc gtaggccgtc tcggccagcg cgtggcgctc
     3781 gcgcggaccc agctcgcggt gcttgcggaa gaagttcgag acgatgacgt cggccggcat
     3841 gtcgagcttg ccgacctcgc gcagcagttc ggtggcggtt tcgaggaggg cgttcggatg
     3901 catggcggag tctttcgcgg ctgtcgctga aatgaagccc ggacggcgca ccgtgcgccg
     3961 ctggagcggg gaaaaaaaac gtcgtgcgcg ggcgctcgtg cgcccacgcg ggagagtcag
     4021 gcggccggtt cggccagcca gccttgcggc tcgtggccgc gatgcgtcac gcggccgtcc
     4081 ttcagttcga gcgcgccttc caggaaccag cggacggcgc gcgggtagag cacatgctcg
     4141 gccgcgagca cgcgcgcgga gagcgtcgcc tcgtcgtcgc ccgccagcac cggcaccacg
     4201 gcctgggcga cgatcgggcc atggtcgagt tcggcgctga cgaaatgcac cgtcgcgccg
     4261 tgcaccttgc agccttcctc gagcgcccgg cgatgcgtgt gcaggccgga gaacgacggc
     4321 agcagcgacg ggtgcacgtt gagcatgcgg ccttcgtagc gcgccacgaa ctgcggcgtc
     4381 aggatgcgca tgaagccggc cagcacgacg aggtcgggcg cgaagccgtc gatggtctcg
     4441 gccagcgcgg cgtcgaaagc cgggcggtcg atgtaggcct tgtggtccag caccgcggtg
     4501 gcgatgccgt ggttctgtgc gaactggaga ccgcgcgcgt cggcgcggtt gctgatgacg
     4561 gcgaccacgc ggcccggcca gtcctgggcc tgggaggcgc gcacgatcgc tcccaggttc
     4621 gagccctgac ccgatatcag gaccgcgatt cgtttcatgg ggcgacattc taagggacaa
     4681 cccgacattc cgcccccggg ggcatggtcc acgctcacta caatcgccct ccccgagcgc
     4741 ccatttcgag attcccatgc gagcccgcgt cctgtccggc atgcgcccca ccggcgccct
     4801 gcacctcggc cactaccacg gcgccctgaa gaactgggtg cggctgcagc acgagtacga
     4861 cagcttcttc ttcgtggccg actggcacgc gctcaccacg cactaccagg atcgcgacgt
     4921 catcgagcgc aacgtctacg acatggtgat cgactggatc gccgccggcc tcgaccccga
     4981 cctgtgcacg atcttcatcc agagccgcct gcccgagcac gccgagctgt tcacgctgct
     5041 ggcgatgggc acgccgctgg gctggctcga gcgcgttccg acgtacaagg atcagatcga
     5101 gcacctgtcg gaccgcgacc tggccaccta cggcttcctc ggctacccgc tgctgcaggc
     5161 ggccgacatc ctgatctaca aggccgcgta cgtgccggtg ggcgaggacc aggactcgca
     5221 cgtcgagctc acgcgcgagg tggcgcgccg gttcaaccac ctgtacggcc gctcgccgcg
     5281 cttcgaggcc caggtggccg ccgcgctcgc caagctgccg ccggagcagc gcgacctgct
     5341 ggagcgccag cggcgccagt tcgggcagga cggcgacgtc gccgcgctcg gcaacggcga
     5401 ggcgctggtc cgcgcccacg ccggccacct gagcagcgag gacgccgaac tgctgctggg
     5461 ccatctcaag ggcagcggcc gcagcgtgct gcacgagccg caggccctgc acacggaagt
     5521 gacgaagctg cccggcctcg acggccagaa gatgagcaag agctacggca acgcgatcga
     5581 gatgcgcgag gagcccgcgc aggtcgaggc caagatccgc aggatgccga ccgacccgca
     5641 gcgcgtgcgc cgcacggacg ccggcgaccc ggcgcgctgc cccgtctggc agttccacaa
     5701 ggtctattcc gacgcggcca cgcagcagtg ggcgcacgac ggctgcacca ccgcgggcat
     5761 cggctgcctg gactgcaagc agccggtcat cgacgccatc ctgcgcgagc agcggccctg
     5821 gcgcgagcgc gcgcagcagt acctggccaa ccccaagcag gtgcactgga tcgtcgagat
     5881 gggcaccgag cgcgcgcgca ccgtcgcgcg ccagacgatg aaggacgtcc gcgacgtcat
     5941 gggactctcg tattgaactc cgccgccgac gcccccatcc tcccgatcgc catggccggc
     6001 atccacatca ccgacatcga gtccgccatc aactggtggc gcgcgcagtt cccgtcgccc
     6061 gacggcatca cggcgtgccc gcaggtgctg gcgctggccg aggtctacgc gctgatggtc
     6121 tattaccgcg agcacgaggc cgacgagttc acgctgcccg cggtggcgcg cgaggcctgg
     6181 ctggagtggt acgcgtcgac gcccgacgcg ccgtgcatcg cgatctgctc cacgtcgcag
     6241 ggcgaccacg agtgcaaagg ctgcggccgc accttcgacg aggtgcagca ctggacggcg
     6301 atgtcgcccg gccagaagcg cgccgtctgg cgccgcatca cgctcgacgc caaggcgtgg
     6361 cgcttcaaca agtacgccga gcgggcgcac aaggccaccg gcgaggatca tcccgatccc
     6421 gcccagctgg acgccgccgc gccggcgaga tcctgagcgc gccgcaggcc ggccgccggc
     6481 ccggcgcagc gactcaggcc gcgatcgacg ccggcaggct gatgacgaag cagccgccgc
     6541 cctcgggcgg ccggacgcag cggatgcgcc cgtggtggcg ctccacgatc tgtcgcacca
     6601 gcgccaggcc cagcccgacg ccgcccgcac gctccgcgtg gcccggcagg cggaagaacg
     6661 gctcgaagat gcgctcgcgg aatgcgtcgg gcacgccggg gccgttgtcg gacacgcgga
     6721 tctcgatgcc ggccgcggcc ttgtcctcga ggtcgacgcg gatctgcccg ccgccgtagc
     6781 gacgcgcgtt ctcgagcagg ttgcgcagcg cgcgccgcag cagccgctcc tcgcccttga
     6841 cgcaggccgg cgcgccctcg acgctggcat cgacgcgcgc gccctcctcc accgcgagcg
     6901 cgagcaggtc gacgctctcg aggttggtcg gctccgacgc ggcctcgagg cggctggcga
     6961 gcagcacctc gtcgaccagg ccgtcgagct cggcgatgtc ggtgccgatc tcggcgcgca
     7021 ggtgttcgcg ctcgcgctcg gagaccgccg gatcggacat cagcgacgtg gccatcttca
     7081 ggcgcgccag cggcgagcgc agctcgtggc tggcgttggc gagcaggctc ttgttggacc
     7141 gcaccagcgc ctcgatgcgc tcggccgcct ggttgaagct ggacgcgagc gtggcgacct
     7201 cgtcgcggcc ctcgacttcc acgcgccgat cgagcttgcc ctggccgaag gcctcgacac
     7261 cgcgcttgag cgtctcgagc cggcgcgtga ggcggcgcac gaacggatag gcgccgatcg
     7321 cgatggccat gaacagcacg accagcagcg ccagcaggct caggccgggc gacacgccgc
     7381 gcggcacgac gccggggaac aggaacgccg ggggcccgct ccgctcggtc gcctgcgggt
     7441 ggtgctcggt gccgaagttg ccgatgtcct cgtcgggcgg catcgccggg tcgttgccgc
     7501 ctggcggctt gagcatgttg ggccgcatca cgtacagcga gcggccgtcg tccagcttga
     7561 tctggtagat cgccacgtgc atcagcgcct tgtcggcctc gcggcgcacg aacgagtcgg
     7621 aggcggcgat gcggtggccc tgctcgtcct ccagcgccat cggcacgcgc aggcgctgcg
     7681 accagtcacg caaggcctcg gcctgcacgt ccggcggcgc tgcggcggcg ggcagcgagc
     7741 gctggatcag gtcgccccag gccgcgatgc gctcggtcag cgcggcctgg ttgcgcgcgt
     7801 gctcctggtc gaggtggcgc tgcacgagcc agcccgacgc gccggcgaac agcgccagcg
     7861 tgacgatcac ggtgagccag atgcgcaggt agagcgaacg caagacgagc cttcagcaga
     7921 gtcagaaacg aaaaagcgcc ggagacatgg agccctcacg ctccctccgg tcgctgctcc
     7981 ccgaggggga gctcggctcg gctcgggtgg tgaccctcgc ttcccgagag ctcgaatata
     8041 cggccacgcg agctgctgga gcttgcgtag aaggcgtcgt caggcgtcct gcttgcgggc
     8101 gaagaggtag cccgagccgc gcaccgtcag cacgcgcttg gggttcttcg ggtcttcctc
     8161 gatcacgctg cggatgcgcg agatgtgcac gtcgatcgag cggtcgaagg cctccagcgg
     8221 gtggcccttg agcgcgtcca tgatctggtc gcggctcagc acgcggccgg ggctgcgcgc
     8281 gagcaccacc agcaggtcga actggtggct ggtgaggtcg cagcgcacgc cgtccaggcg
     8341 cgcctcgtgg gcaccgaggt cgatctccag cttgccgaag cgcaggactt cctcgttgac
     8401 cggctcgggg ctggcgcggc gcagcagcgc cttgacgcgc gccagcagtt cgcgcggctc
     8461 gaagggcttg ggcaggtagt cgtcggcgcc gagctccagg ccgacgatgc ggtcggtggg
     8521 ctcgccgcgc gccgtcagca tcatcagcgg caggcggcgg gtggccggct ggccgcgcaa
     8581 ctcgcgcgtg aaatcgaggc cgtcgccgtc gggcagcatc aggtcgagga cgagcgcgtc
     8641 gtacgagccc gcggccaggc gcatgcggcc gtcggccagc gtcccggcgg tgtcgacgtc
     8701 gaagccctgg gtgcacaggt agtcgccgac catgcgcgtc aggcgggcat cgtcgtcgat
     8761 cagcaagagg tgcggattgg tcatggaggc gtgggcgttc cgcgaggaag aagcgacagg
     8821 gcggacgccc tgcgcgctgg gagcgtaacc gattgcgtcg aaggatgtca tggggtcgtt
     8881 cgctggcggc gggaggccgc gtcgaggtgg gagtggatgg gcgcgcgccg gtcagcgcgt
     8941 ggacgccccg tcggaccccg cccgcggaag ccgcgcgcga cgccgcggcg aggtgccggc
     9001 ggggcctgcg ggagtccggg cataacgagt cattgcaggc gatcctgccc ggccgcggtc
     9061 aaccgggctt gcgcgcctcg taaagt
//
LOCUS       DATCRO010000653         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_547647, whole genome shotgun sequence.
ACCESSION   DATCRO010000653 DATCRO010000000
VERSION     DATCRO010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547647"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIN75_17875"
     CDS             <1..361
                     /locus_tag="VIN75_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8976090.1"
                     /translation="TIAHHALIAGNRGKTIAFMWMTVALGFIFVCLQAYEYHHAYTEL
                     NLKLSSGTYGSTFFMLTGFHGLHVFVGMLMLFFITLRLQKGHFTPKRHFGFEGAAWYW
                     HFVDVVWLGLYAVVYWL"
     gene            complement(561..782)
                     /locus_tag="VIN75_17880"
     CDS             complement(561..782)
                     /locus_tag="VIN75_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HEY8976091.1"
                     /translation="MKFVIVGFLVLVLAALAGAGLFMLRRGPDDGSADKRMARALAIR
                     VALSVTLFLLILLAWSLGWIHPKGIPVGT"
     gene            781..1629
                     /locus_tag="VIN75_17885"
     CDS             781..1629
                     /locus_tag="VIN75_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY8976092.1"
                     /translation="MTNDYSTDPAPAPAGSSPPRRGAGSLRSRIVWFVVALACASLTA
                     QLGRWQLSRAHAKLAYEALVAERGGLPPMPGTALARDAATGEQQWQRHITLSGQWDAA
                     HTIFLMNRTMDERAGFYVLTPLRLPDGGAVVVQRGWVASDPADPTKAPPVPTPIGPVT
                     LHGHVAPWPSHWFEVGHGRGGAVRQNLELAPFSAESGLALRPVTVFEDANADNAGDGL
                     RRNWPAPTAGVSIATHYGYATQWFAMSVAFLGLWVWLQFIRPRRRSPDDSDFPPGARA
                     DDPAPR"
     gene            1626..>2198
                     /locus_tag="VIN75_17890"
     CDS             1626..>2198
                     /locus_tag="VIN75_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976093.1"
                     /translation="MKPTPPDDPKATGPESMTFTLHGTPAPMRDYPAAQSRRGRIMML
                     VILAICALPVIASYFTYYVIRPGGRTNYATLVEPQRPLPSLEALPAQDMDGRSVPLTT
                     LKGQWLLVVVAPASCGKDCERNLFLQRQLREMMGAERDRLQKVWLVDSPEPVAPALAS
                     AVGAPPAVLSYRVDRVALARWLEPDRGHALE"
BASE COUNT          307 a          798 c          756 g          337 t
ORIGIN      
        1 gaccattgcg caccacgcgc tgatcgccgg caaccgcggc aagaccattg ccttcatgtg
       61 gatgacggtc gcgctgggct tcatcttcgt gtgcctgcag gcctacgagt accaccatgc
      121 ctacaccgag ctcaacctca agctcagctc gggcacctac ggctcgacct tcttcatgct
      181 cacgggcttc cacggcctgc atgtgttcgt gggcatgctg atgctgttct tcatcacgct
      241 gcgcctgcag aaggggcact tcacgcccaa gcgccacttc ggctttgaag gcgcggcgtg
      301 gtactggcac ttcgtcgacg tcgtgtggct gggcctgtac gccgtggtct actggctttg
      361 aatccgtagg gggcgggtca gccacgggcg tcacccgccg ttcgtggacg tcgcgactgg
      421 ccggccggcg ggttacgcct gcggctaacc cgccctacga agctcggtgc tgtcccggca
      481 ggagagacgc cgcctcggaa tctcgggcgg cgtcgtcgtt tgaggagagc ggaaccacgc
      541 ggcggctgcc gcgtcgaaag tcaggttccg accgggatgc ccttcggatg gatccagccc
      601 aggctccagg ccagcaggat cagcaggaac agggtgacgg acaatgcgac gcggatcgcg
      661 agggcgcggg ccatgcgctt gtcagccgat ccgtcgtccg ggccgcggcg cagcatgaac
      721 agcccggcgc cggccaacgc ggccagcacg agcaccagga acccgacgat cacgaatttc
      781 atgacgaacg attattccac cgaccccgcg ccggcgcccg cgggttcctc ccctccgcgg
      841 cggggcgcgg gcagcctgcg ctcgcgcatc gtctggttcg tcgtcgcgct ggcgtgtgca
      901 tcgctgacgg cgcagctggg ccgctggcag ctgtcgcgcg cgcacgccaa gctcgcgtac
      961 gaggccctgg tggccgagcg cggcggcctg ccgccgatgc ctggcacggc gctcgcgcgc
     1021 gacgcggcca cgggcgagca gcagtggcaa cgccacatca cgctgtccgg gcagtgggac
     1081 gcggcgcata ccatcttcct gatgaaccgc acgatggacg agcgcgcggg cttctacgtg
     1141 ctgacgccgc tgcgcctgcc cgacggcggc gccgtcgtgg tgcagcgtgg ctgggtcgcc
     1201 agcgatccgg ccgatccgac gaaggcgccg ccggtgccca cgccaatcgg ccctgtcacg
     1261 ctgcacgggc atgtcgcgcc gtggccttcg cactggttcg aggtcgggca cggacgcggc
     1321 ggcgcggtcc ggcaaaatct cgagctggcg ccgttcagcg cggagtcggg cctggccttg
     1381 cgtcccgtca cggtgttcga ggatgccaac gccgacaatg ccggcgacgg cctgcgccgc
     1441 aactggcccg cgccgaccgc cggcgtgagc atcgccaccc actacggcta tgccacgcag
     1501 tggttcgcga tgagcgtggc gttcctcggc ctgtgggtgt ggctgcaatt catccgcccg
     1561 cgccgccgct cgcccgacga ttccgatttc ccgcctggcg cccgcgccga cgatcctgct
     1621 ccccgatgaa accgacgccg cccgacgacc ccaaggccac cggcccggaa tccatgacct
     1681 tcacgctgca cggcaccccc gcgcccatgc gcgactaccc ggcggcgcag agccggcgcg
     1741 gccgcatcat gatgctggtg atcctggcca tctgcgcgct gccggtgatc gcgtcgtact
     1801 tcacgtacta cgtcatccgc ccgggcggcc gcacgaacta cgccacgctg gtcgagccgc
     1861 agcgcccgct gccttcgctc gaggcgctgc cggcgcagga catggacggc cgctcggtgc
     1921 cgctgaccac gctgaagggc cagtggctgc tggtggtcgt cgcgcccgcg agctgcggca
     1981 aggactgcga gcgcaacctg ttcctgcagc gccagttgcg cgagatgatg ggcgccgagc
     2041 gcgaccgcct ccagaaggtg tggttggtcg acagccccga gcccgtcgcg cccgcgctgg
     2101 cgtcggccgt cggcgcgccg ccggcggtgc tgagctaccg cgtcgaccgc gtcgcgctgg
     2161 cgcgctggct ggagcccgac cgcggccacg cgctggag
//
LOCUS       DATCRO010000654         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_929808, whole genome shotgun sequence.
ACCESSION   DATCRO010000654 DATCRO010000000
VERSION     DATCRO010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929808"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VIN75_17895"
     CDS             <1..903
                     /locus_tag="VIN75_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976094.1"
                     /translation="KPVVLPLPPAPDLSAVPAQFDHLPIRFPAPGVLRVRVQVADDAA
                     FDHIQYDQHFDSGDDVYIPQLADGTWHLRVRAISPEGLEGLDAVRDFRLLARPEAPFL
                     TEPPAGGKRPVGDVTLRWTHNPDAAAFVVEVARDAKFTDLALHDEHVSGETAVFHPTD
                     SAFGAADGLYWWRVQSVAADGRRGAWSEPQALVLRPTPRAPLGQVSPDGAAIELRWGG
                     RPEDRAEAELASDAGFRQVVRRGDYETPGTRLPRPAAGTYFARYRFVEPDGFKTPWSA
                     PVKIEVEGSWHRALGALLPASWLK"
     gene            complement(1059..1835)
                     /gene="cysE"
                     /locus_tag="VIN75_17900"
     CDS             complement(1059..1835)
                     /gene="cysE"
                     /locus_tag="VIN75_17900"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY8976095.1"
                     /translation="MFDRLREDITTILERDPAARSRLEVLLCYPGLHALVWHRRAKWF
                     WTHGMHVAGRFLSHLGRFFTGIEIHPGATIGRRVFIDHGMGVVIGEMAEIHDDCTIYQ
                     GVTLGGTSLVKGAKRHPTLEAGVIIGANASVLGGFTVGAGARVGSGAVVTRPVPAGAT
                     AVGNPARIIAAETDAAREATAARLGFSAYGVTQGDDPIAQAMKGLIDNASAHEHQIAL
                     LWTAIERVLAEGLAQARDCVPTDAQVDEKFDAEGLSRLVK"
     gene            2172..3452
                     /locus_tag="VIN75_17905"
     CDS             2172..3452
                     /locus_tag="VIN75_17905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976096.1"
                     /translation="MPFANLYLVAAVVVVGVAFAALARLLPARAAKPAASGPPESSRH
                     AALQGFRGLLAFGVFMHHSFLWSRYGRGFGWIDADVWHQFGESRVVLFFMLTATLFYG
                     RLLDARGRGLDWLKLYVSRLLRLGPAYWLAMALMFAAIAWATLHRIDGAGTSIVRFSW
                     SDILGECLAWLGFSILGMPAIDGYFATPTVTAVVTWTLPYECAFYALLPLLALPLRVK
                     MPLTTLALGLAGAIGLAVSDADPVYCVPFAGGLAVAQAMRIPRLAGVLRHRACAAAAM
                     LGMTVAILEFHTIYAPVPLLLLAFSLAAVACGNDLFGILNWRLPQALGSWAYSLYLLH
                     GIVLYAVFKLVVGDERAVHLTDRECGLMLAGLVPVVLAIAWASQRWVESPPMRAVPAV
                     VAAIRKRLSRRATAPIEAVVALPAAGVAAERHAA"
     gene            complement(3456..4184)
                     /locus_tag="VIN75_17910"
     CDS             complement(3456..4184)
                     /locus_tag="VIN75_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8976097.1"
                     /translation="MTDPTRFILVDTSHPGNVGASARAMKVMGFSDLVLVTPRFADVL
                     TQPDAIAMASGATDILERARIVDTLDEALAGTTFACATAMTPRDFGPPTRAPREAFAE
                     LARTDHRVAFVFGGERFGLSNEHVYACHVCISIPTQPEYGSLNLAQAVQLLSYDWRQA
                     LGGFPVVERTRTPDQADAAAVRGLLGHWREMLVEIDFLDPAVPRKLMARLHQLVNRAQ
                     VTTEEVHILRGIARQVQKLRRSGR"
     gene            4401..5366
                     /locus_tag="VIN75_17915"
     CDS             4401..5366
                     /locus_tag="VIN75_17915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8976098.1"
                     /translation="MSTNLHPMLNIAVKAARAAGAIINRASLDVDKVQVEQKGVNDFV
                     TEVDKHAEAVIVETILQAYPGHGILAEESGRELGAKDSEFVWIIDPLDGTTNFIHGFP
                     VYAVSIALAWRGVVQQAVVYDPNRNDLFYASRGRGAFLNDRRIRVSKRLRLADSLIGT
                     GFPFRKGDNFKRYMKMFEEISQQCAGLRRPGAASLDLCYVAAGYYDGFFETGLSPWDI
                     AAASLVITEAGGLIGNFSGEADFLYQREVVAGNPKVYGQLVNILAPYSRVIKDDDKPA
                     AAEGAVPDATDAFVAANAEAAPAAPAENKPRKAIKVTGPRTPRKA"
     gene            5485..5742
                     /locus_tag="VIN75_17920"
     CDS             5485..5742
                     /locus_tag="VIN75_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976099.1"
                     /translation="MPELRLNVPQSVIDQINEQINKINGNPPGTQLSANDIGREALAV
                     YKWAVNQTNAGYAVVSADANRSPVVQIATEHLPARAPTSVR"
     gene            5761..6102
                     /locus_tag="VIN75_17925"
     CDS             5761..6102
                     /locus_tag="VIN75_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976100.1"
                     /translation="MATGPQAGWSVDTEEVAPVGDAASSDKRSQRGMRETITIWLLVL
                     LCVVVGLAFVVVLYDVDPAIADKRFEHLKNLLDVMLGPIVTLLSSVIGFYFGSQVAQD
                     KAQSGESGKDK"
     gene            6103..>6650
                     /locus_tag="VIN75_17930"
     CDS             6103..>6650
                     /locus_tag="VIN75_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8976101.1"
                     /translation="MPADKKASGWPEVFLKWIEAVALRLAPLLVLFALFGCVVLTVRW
                     LRAASPPAGPTVIVLGPDAKGVERKVFAADSLFEYKSKALNPKSEREIADFAAQLRDS
                     HVAQVIVIGHTDRIGGAAYNTRLSQARAEAVREALLRSGLRADRVLAVGIGNRMPATA
                     KDECPGTDKDATTIACLAKDRRV"
BASE COUNT          945 a         2377 c         2336 g          992 t
ORIGIN      
        1 aagccggtgg tgctgccgct gccgccggcg cccgacctgt cggccgtgcc ggcgcagttc
       61 gaccacctgc cgatcaggtt ccccgcgccc ggcgtgctgc gcgtgcgcgt gcaggtggcc
      121 gacgacgccg cgttcgacca catccagtac gaccagcact tcgactcggg cgacgacgtc
      181 tacatcccgc aactggccga cggcacctgg cacctgcgcg tgcgcgccat cagccccgaa
      241 ggcctcgagg gcctggacgc ggtgcgcgac ttccggctgc tggcccggcc cgaggcgccc
      301 ttcctcaccg agccgccggc cggcggcaag cggccggtgg gcgacgtcac gctgcgctgg
      361 acgcacaacc ccgacgccgc cgccttcgtc gtggaggtcg cgcgcgacgc gaagttcacg
      421 gatctcgcgc tgcacgacga gcacgtcagc ggcgagacgg ccgtcttcca cccgaccgac
      481 tccgccttcg gtgccgccga cggcctgtac tggtggcgcg tgcagagcgt ggcggccgac
      541 ggccgccgcg gcgcctggag cgagccgcag gccctcgtgc tgcgccccac cccgcgcgcg
      601 cccctgggcc aggtgagccc cgacggtgcc gccatcgagc tgcgctgggg cggccggccc
      661 gaggatcgcg cggaggccga actggccagc gacgccggct tccggcaggt cgtcaggcgc
      721 ggcgactacg agacgcccgg cacccgcctg ccgcgccccg ccgccgggac gtacttcgcg
      781 cgctaccgct tcgtcgagcc cgacggcttc aagacgccgt ggagcgcgcc ggtgaagatc
      841 gaggtcgagg gcagctggca ccgcgcgctc ggtgcgctgc tgccggcgtc ctggctgaag
      901 tagcgctcag ccgagccggc gcacgcccgc gggcgtcgcg atgtcggccg cggacaggcc
      961 ggcgcccgcg gaggcgacgc cgacgaccga cctcacatga tcggcgccgc tttcgagcga
     1021 cggcgccgtg accacgagat ggtcgatctc gcagcgcgct acttgaccag gcggctcagg
     1081 ccttcggcgt cgaacttctc gtcgacctgg gcgtcggtgg gcacgcagtc cctggcctgc
     1141 gccaggcctt cggccagcac gcgctcgatg gcggtccaca acagcgcgat ctggtgctcg
     1201 tgcgccgacg cgttgtcgat caggcccttc attgcctgcg cgatcggatc gtcgccctgc
     1261 gtcacgccgt aggccgagaa gcccaggcgc gcggcggtgg cctcgcgcgc cgcgtcggtc
     1321 tcggcggcga tgatgcgggc cgggttgccc accgcggtgg cgccggccgg cacgggacgc
     1381 gtgacgaccg cgcccgagcc cacgcgggcg ccggcaccga cggtgaagcc gcccagcacg
     1441 ctggcgttgg cgccgatgat cacgcccgcc tccagcgtcg gatgccgctt ggcgcccttg
     1501 accagcgagg tgccgcccag cgtcacgccc tggtagatgg tgcagtcgtc gtggatctcg
     1561 gccatctcgc cgatgaccac gcccatgccg tgatcgatga agacacgccg gccgatagtc
     1621 gccccggggt ggatctcgat gccggtgaag aagcgcccca ggtgcgacag gaagcgtccg
     1681 gccacgtgca tgccgtgcgt ccagaaccac ttggcgcggc gatgccagac cagcgcgtgc
     1741 aggcccgggt aacagagcag cacctccagg cgcgagcgcg cggcggggtc gcgctcgagg
     1801 atggtggtga tgtcttcacg caggcgatcg aacattgcgg gcagtctcgc ggctcgtgga
     1861 atcggaaggg aacggggagt gtatcggcag ccccgccgca tcgccggccg gggccgggat
     1921 cgccccatcg ccaggcggtc caagcgggcg cgcagggcgg cgtcagatct gcgacagggc
     1981 ggggacagct gcgtgatgcc ggacaggacg aagacagtct ggggcgctcc acttacaact
     2041 gcgcggccat accacgccgg gatcgggtct aatgtcacga accttcgatg aaccggacat
     2101 tggtcacgaa gcatcggaat actggacagg ccgcctcgca gaaggtgccg ccaggagagg
     2161 gagagggatt cttgccgttc gccaacctgt atctcgtcgc ggccgtggtc gtcgtgggcg
     2221 tcgcctttgc ggcgctcgcg cgcctgctgc ctgcgcgcgc ggccaagccc gccgcctcgg
     2281 gtccacccga gtcctcgcgc cacgccgcgc tgcagggctt ccgcgggctc ctggccttcg
     2341 gcgtgttcat gcaccactcg ttcctctgga gccgctatgg acgcggattc ggctggatcg
     2401 atgccgacgt ctggcaccag ttcggcgaaa gccgcgtcgt gctgttcttc atgctcaccg
     2461 cgacgctgtt ctacggccgc ctgctcgacg cccgcggccg cggcctggac tggctcaagc
     2521 tctacgtctc gcgcctgctg cgcctggggc cggcctactg gctcgcgatg gcgctgatgt
     2581 tcgccgccat cgcctgggcg accctgcacc gcatcgacgg cgccggcacg tccatcgtca
     2641 ggttctcctg gtccgacatc ctcggtgagt gcctcgcgtg gctgggtttc tccatcctgg
     2701 gcatgcccgc catcgacggc tacttcgcca cgccgaccgt gacggccgtc gtcacgtgga
     2761 cgctgcccta cgaatgcgcc ttctacgcgc tgctgcccct gctggcgctg ccgctgcgcg
     2821 tgaagatgcc gctgacgacg ctcgcgctgg gcctggccgg cgccatcggg ctggcggtgt
     2881 cggacgccga tccggtctac tgcgtgccct tcgcgggggg cctggccgtg gcccaggcca
     2941 tgcgcatccc gcgcctggcc ggggtgctgc gccaccgcgc ctgcgccgcc gccgcgatgc
     3001 tggggatgac cgtcgcgatc ctggagttcc acaccatcta cgcgcccgtg ccgctgctgc
     3061 tgctggcgtt ctcgctggcg gcggtggcgt gcggcaacga cctgttcggc atcctgaact
     3121 ggcgcctgcc gcaggcgctg ggcagctggg cctacagcct gtacctgctg cacggcatcg
     3181 tgctctacgc ggtgttcaag ctggtggtcg gcgacgagcg cgccgtgcac ctgaccgacc
     3241 gggagtgcgg cctgatgctc gccgggctgg tgccggtggt gctggcgatc gcctgggcca
     3301 gccagcgctg ggtcgagtcg ccgccgatgc gcgcggtgcc ggccgtcgtc gcggccatcc
     3361 gcaagcgcct gtccaggcgc gcaaccgccc cgatcgaggc cgtcgtcgcg ctgcccgccg
     3421 ccggcgtcgc ggccgagcgc cacgcggcgt gagcttcagc gcccgctgcg gcgcagcttc
     3481 tggacctggc gcgcgatgcc gcgcaggatg tgcacttcct cggtggtcac ctgcgcgcgg
     3541 ttgacgagct ggtgcagccg cgccatcagc ttgcgcggca ccgccgggtc gaggaagtcg
     3601 atctccacga gcatctcgcg ccagtggccc agcaggccgc gcacggcggc ggcgtcggcc
     3661 tggtccggcg tccgcgtgcg ctcgacgacg ggaaagccgc cgagcgcctg gcgccagtcg
     3721 taggacagca gctgcacggc ctgcgcgagg ttgagcgatc cgtactccgg ctgggtcggg
     3781 atgctgatgc agacgtggca ggcgtagaca tgctcgttgg acagcccgaa gcgctcgcct
     3841 ccgaacacga acgcgacgcg atggtcggtg cgcgccagct cggcgaaggc ctcgcgcggc
     3901 gcgcgcgtgg gcgggccgaa gtcgcgcggc gtcatcgcgg tggcgcaggc gaaggtggtg
     3961 ccggcgagcg cctcgtccag cgtgtcgacg atgcgggcgc gctcgaggat gtcggtggcg
     4021 ccggacgcca tcgcgatcgc gtcgggctgc gtcagcacgt cggcaaagcg cggcgtcacc
     4081 agcaccaggt cggaaaagcc catcaccttc atggcgcggg cggaggcgcc gacattgccg
     4141 ggatggctgg tgtcgaccag gatgaagcgc gtcggatccg tcaagaactg caccgtggca
     4201 agtggacggc acgggcgggc gcgtgacttc gcggggccgg gccggataga atggaggatt
     4261 gcgcattgtc gcgccgccgt cgcttgcgtc gaccgccgcc cggcatccgc ccgctctttc
     4321 gtcgctgccg ccctcttccg ccctcgccgg cgctccccct gcggagccgc cgctggcgtc
     4381 accttttcag gaatcgtccc atgtccacga acctgcaccc gatgctcaac attgccgtca
     4441 aggccgctcg cgcggcgggg gcgatcatca acagggcgtc gctcgacgtg gacaaggtgc
     4501 aggtggaaca gaagggcgtg aacgacttcg tcaccgaggt cgacaagcat gcggaagcgg
     4561 tcatcgtcga gaccatcctc caggcctatc cgggccacgg catcctggcc gaggaatcgg
     4621 gccgcgagct cggcgccaag gacagcgagt tcgtctggat catcgacccg ctggacggca
     4681 ccaccaactt catccacggc ttcccggtct acgcggtctc gatcgcgctg gcctggcgcg
     4741 gcgtggtgca gcaggccgtg gtctacgacc cgaaccgcaa cgacctgttc tacgcctcgc
     4801 gcggccgcgg cgccttcctc aacgaccgcc gcatccgcgt ctccaagcgc ctgcgcctgg
     4861 ccgactcgct gatcggcacg ggcttcccgt tccgcaaggg cgacaacttc aagcgctaca
     4921 tgaagatgtt cgaggagatc tcgcagcagt gcgccggcct gcgccgccct ggcgcggcct
     4981 cgctggacct gtgctacgtg gccgcgggct actacgacgg tttcttcgag accgggctga
     5041 gcccgtggga catcgccgcc gcctcgctgg tcatcacgga agccggcggc ctgatcggca
     5101 acttcagcgg cgaggccgac ttcctgtacc agcgcgaggt cgtcgccggc aacccgaagg
     5161 tctacggcca gctcgtcaac atcctggcgc cctactcgcg cgtcatcaag gacgacgaca
     5221 agcccgcggc cgccgagggc gccgtgccgg acgccaccga cgccttcgtc gccgccaacg
     5281 ccgaggcggc acctgccgcc cctgccgaga acaagccgcg caaggccatc aaggtcaccg
     5341 gcccgcgcac cccgcgcaag gcctgagcgg cgcggaatcg ggcccggggc gtgaccgggc
     5401 cggtcgatgt cgcccctttg tctgccggtt ggttttggag taatattgga cgaatcacgg
     5461 atgatttatc caatggagaa tgcgatgccc gaactccgcc tcaacgttcc gcaatcggtg
     5521 atcgaccaga tcaacgagca gatcaacaag atcaacggca acccgcccgg gacgcaactg
     5581 tcggccaatg acatcggccg ggaggcgctg gcggtctaca agtgggcggt gaaccagacc
     5641 aacgcaggtt acgcggtggt ctcggccgac gccaaccgct cgccggtcgt gcagatcgcg
     5701 accgagcacc tgcccgcacg cgcgcccacc tccgtccgct gacccaggca aggccgggcg
     5761 atggcgaccg ggccccaggc cggctggagc gtcgacacgg aggaggtggc gccggtcggc
     5821 gacgcggcct cgagcgacaa gcgttcccag cgcggcatgc gcgagacgat caccatctgg
     5881 ctgctggtgc tgctctgcgt cgtcgtcggc ctggcgttcg tcgtggtgct ctatgacgtc
     5941 gatccggcca tcgccgacaa gcgcttcgag catctcaaga acctgctgga cgtcatgctg
     6001 gggcccatcg tgaccctgct gtcgtcggtg atcggcttct acttcggctc gcaggtggcg
     6061 caggacaagg cgcagtccgg cgagtccggc aaggacaagt agatgccggc cgacaagaag
     6121 gcatccggct ggcccgaggt cttcctgaaa tggatcgagg ccgtggcgct gcgcctggcg
     6181 ccgctgctgg tgctgttcgc gctgttcggc tgcgtggtcc tgaccgtccg gtggttgcgt
     6241 gccgcatcgc cgccggcagg ccccacggtg atcgtgctgg gccccgacgc gaaaggcgtc
     6301 gaacgcaagg tcttcgcggc cgactcgctg ttcgagtaca agtccaaggc gctcaacccg
     6361 aagagcgaac gcgagatcgc ggatttcgcg gcgcagctca gggacagcca cgtcgcgcag
     6421 gtcatcgtga tcggacacac tgaccggatc gggggtgcgg cctacaacac ccgcctgtcg
     6481 caggcgcgcg ccgaggccgt gcgcgaagcg ctcctgaggt cgggcctccg ggcggacagg
     6541 gtcctcgccg tcggcatcgg caaccgcatg cccgcgaccg cgaaggacga atgccccggc
     6601 accgacaagg acgccacgac gattgcctgc ctggccaagg atcgccgggt
//
LOCUS       DATCRO010000655         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_288208, whole genome shotgun sequence.
ACCESSION   DATCRO010000655 DATCRO010000000
VERSION     DATCRO010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288208"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..741
                     /locus_tag="VIN75_17935"
     CDS             43..741
                     /locus_tag="VIN75_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976102.1"
                     /translation="MIPSSRPALSALALLTSLLLGGVATSARAVALDNGFSGQNFLDT
                     PLAGTTSAARPELAGTVLADTDTPFSLDGVTGYVQNRVVRETATGTLDFYWRVVVDPG
                     SGSGSGIEALRLTDFGYGYMTDADWRIDGSGSVGATTGRLFNPGSYPQGDINFLFASN
                     VDAGESSSFFFLHTGATAFAQTGGYDVLNADGTFTAIASTYAPAVPEPTPAMLLALGV
                     LTLGLLRSRRARQD"
     gene            complement(820..>2005)
                     /locus_tag="VIN75_17940"
     CDS             complement(820..>2005)
                     /locus_tag="VIN75_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEY8976103.1"
                     /translation="RNHVTLRIEPGATLRGSQDDADYPFLNPPVANTQLADTRHALVY
                     AESATGVHVEGGGTIDGNADIEKWRGMSRPEGQRPMAIYTALSKDVTIEGVTVRDAGV
                     WAVVNMQVEHLVIRGITVDSPHGPTHDGIDVVDGHDVLIEDNTINAGDDGICLKSGST
                     KGLSHVVVRRNHVRGAGVANGLKLGTATVGPMRDIVFEDIRVENAQAAAMAVESVDGG
                     EVSDVTFRRVTASDVGTPFFVLLGARGKAGTGAIHDIRFEAIRAANLRYPWGALLSGA
                     PADAAGRHDLRAITFDDVDITWRGAGGAAGPHVFGSGAVDTARFPVYAGGYPDAKFIF
                     ATPSDKSEVTYYALPGYAFFVRDARGVRFGRCRAAVDGVERRDAIARNDAEVSGTCSP
                     AR"
BASE COUNT          264 a          749 c          695 g          297 t
ORIGIN      
        1 ggcctaccgt gaggactcca acaagacacc cacggagtcc ccatgatccc atcgagccgt
       61 ccggccctgt ccgccctcgc cctcctgacg tcgctgctgc ttggcggcgt cgcgacgagc
      121 gcccgcgccg tcgccctgga caacggcttc tcaggccaga acttcctcga cacgccgctg
      181 gccggcacca cctccgccgc gcggcccgag ctcgccggca ccgtgctcgc cgataccgac
      241 acgccgttct cgctcgacgg cgtcaccggc tacgtgcaga accgcgtcgt gcgcgagacg
      301 gccaccggaa cgctggattt ctactggcgc gtcgtggtcg acccgggctc cggcagcggc
      361 tccggcatcg aggccctgcg gctgacggac ttcggctatg gctacatgac cgatgccgac
      421 tggcgcatcg acggcagcgg cagcgtcggc gcgacgacgg gccgcctgtt caacccgggc
      481 tcctaccccc agggcgacat caacttcctg ttcgcatcga acgtcgacgc cggcgagagc
      541 tcgtcgttct tcttcctgca caccggcgcg accgccttcg cgcagaccgg cggctacgac
      601 gtgctgaacg ccgacggcac gttcaccgcc atcgcgagca cctacgcgcc ggccgtgccc
      661 gagccgacgc ctgcgatgct gctggcgctg ggcgtgctca cgctgggcct gctgcgcagc
      721 cggcgcgcca ggcaggactg aatcggcagg cgcccgctcc aaggcaggtg gcttcggcgg
      781 cgcggcgccg tccgcaagcg tccggatgaa ccggatggct cagcgcgccg gcgagcacgt
      841 cccgctgacc tccgcgtcgt tgcgcgcgat cgcgtcgcgg cgctcgacgc cgtcgacggc
      901 cgcgcggcag cgcccgaagc gcacgccgcg cgcgtcgcgc acgaagaacg cgtagccggg
      961 cagcgcgtag taggtcactt cggacttgtc gctgggcgtg gcgaagatga acttggcgtc
     1021 gggatacccg ccggcataca cggggaagcg cgcggtgtcg accgcgccgc tgccgaacac
     1081 gtgcgggccg gccgcgccgc cggcgccgcg ccaggtgatg tcgacgtcgt cgaaggtgat
     1141 cgcgcgcagg tcgtggcggc ccgcggcgtc ggccggcgcg ccggacagca gcgcgcccca
     1201 gggatagcgc aggttcgcgg cacggatcgc ctcgaagcgg atgtcatgga tcgcgcccgt
     1261 gcccgccttg ccgcgcgcgc ccagcagcac gaagaacggt gtcccgacgt cggacgccgt
     1321 gacgcggcgg aacgtcacgt ccgacacctc gccgccgtcg accgattcga ccgccatcgc
     1381 ggcggcctgc gcgttctcga cgcggatgtc ctcgaacacg atgtcgcgca tcggtcccac
     1441 cgtggccgtg cccagcttca ggccgttggc cacgcccgcg ccgcgcacgt ggttgcggcg
     1501 cacgacgacg tggctcaggc ccttggtgct gccgctcttg aggcagatgc cgtcgtcgcc
     1561 ggcgttgatc gtgttgtcct cgatcagcac gtcgtggccg tcgaccacgt cgatgccgtc
     1621 gtgcgtcggc ccgtgcggcg aatcgaccgt gatgccgcgg atgacgaggt gctcgacctg
     1681 catgttgacc accgcccaga cgccggcgtc gcgcacggtc acgccctcga tggtgacgtc
     1741 cttggacagc gccgtgtaga tcgccatcgg ccgctggccc tcggggcgcg acatcccgcg
     1801 ccacttctcg atgtcggcgt tgccgtcgat cgtgccgccg ccctcgacat gcacgccggt
     1861 ggcgctctcg gcatagacga gcgcgtggcg ggtgtcggcc agctgcgtgt tggccacggg
     1921 cgggttcagg aacgggtagt ccgcgtcgtc ctggctgccg cgcagcgtcg cgcccggctc
     1981 gatgcgcagc gtcacgtggt tgcgc
//
LOCUS       DATCRO010000656         7233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_299843, whole genome shotgun sequence.
ACCESSION   DATCRO010000656 DATCRO010000000
VERSION     DATCRO010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299843"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VIN75_17945"
     CDS             <1..437
                     /locus_tag="VIN75_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8976104.1"
                     /translation="ALPRPLLHSGDLDFSFAGLKTAVMTAMRKFGPEPTDRQRADLAA
                     STQAAIVEVLVKKSLRALQETRLSRLVVAGGVGANTRLREHLDGVAARRGLRVHYPEL
                     ALCTDNGAMIALAGALRLQRDPSQARRDYAFDVKPRWPLAQA"
     gene            complement(583..1056)
                     /locus_tag="VIN75_17950"
     CDS             complement(583..1056)
                     /locus_tag="VIN75_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8976105.1"
                     /translation="MFIVPVSRRAAVRARHFDRLFDDAFGHIFAQPAADAAPVRRPAI
                     DVAESDSAYVVTLDVPGVAREDVKVSIDGRAVSIVAEARAETPAADAPTDTTAEAPKA
                     GERVILRERATASFARSFTLQSEIDQAASQAKLDNGVLTLTLAKRSPATTQLTVN"
     gene            1278..1520
                     /locus_tag="VIN75_17955"
     CDS             1278..1520
                     /locus_tag="VIN75_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976106.1"
                     /translation="MAAAIAFGYNPPLVLGVGFHVWKRRQGQIQHAPGRFHSGAQVVK
                     ETEQAPPPLADAAWLRIRVHARSALPVSVQHEGNPQ"
     gene            1517..1780
                     /gene="rpsO"
                     /locus_tag="VIN75_17960"
     CDS             1517..1780
                     /gene="rpsO"
                     /locus_tag="VIN75_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8976107.1"
                     /translation="MTDINKAEIVAAHARGANDTGSPEVQVALLTARINALTPHFKQH
                     AKDHHGRRGLLKMVNSRKSLLAYLKKTDANRYLALIGKLGLRK"
     gene            1989..4174
                     /gene="pnp"
                     /locus_tag="VIN75_17965"
                     /pseudo
     CDS             1989..4174
                     /gene="pnp"
                     /locus_tag="VIN75_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064373.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            4230..5222
                     /locus_tag="VIN75_17970"
     CDS             4230..5222
                     /locus_tag="VIN75_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY8976108.1"
                     /translation="MRAIEIAGVGGPEVLRAVVRARPVAGVGEAVVRVTASGVNRPDV
                     LQRKGHYPVPPGASDIPGLEIAGVIDGGDAAALAAAGFEVGDRVCALVAGGGYAEYCA
                     APVGQMLPVPAGFSDVEAAALPENFFTVWSNVFDRGRLAPGEWLLVQGGSSGIGVTAI
                     QLGRAFGARVIATAGSDDKCARCVELGADHAVNYRTEDFAARALEITGGKGVDVVLDM
                     VAGDYVAREVSCLAEDGRIVLIAVQGGTKSQIDAGLVLRRRLTLTGSTLRPRPVAFKA
                     AIAAALREKVWPLLETRQVQPVVQEVFPAEQAWRAHAAMEEGSHVGKLMLAWGT"
     gene            5335..6075
                     /gene="tpiA"
                     /locus_tag="VIN75_17975"
     CDS             5335..6075
                     /gene="tpiA"
                     /locus_tag="VIN75_17975"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8976109.1"
                     /translation="MRKKLVAGNWKMHGSHTANAELLQGILAARPFVCDVAVCVPFPY
                     LSEVAVALAGSDVRWGAQDCSQHDKGAYTGEVSASMLAEFGCRYAIVGHSERRAMHGE
                     SDLQVAEKAKAVLARGLTPIVCVGETLAQREQGAAQETVKRQLSAVIHALAHCVGEMV
                     VAYEPVWAIGTGVTATPGQAQEMHALIRAQLRAATPHADAMRLLYGGSMKPDNAAALL
                     AQPDIDGGLIGGAALKAADFVAICRAAG"
     gene            6116..6529
                     /gene="secG"
                     /locus_tag="VIN75_17980"
     CDS             6116..6529
                     /gene="secG"
                     /locus_tag="VIN75_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY8976110.1"
                     /translation="MSVWHNFLFILQVLSAMAMIGLVLIQHGKGADMGASFGSGASGS
                     LFGATGSANFLSRSTAICATIFFATTLGMSVLSTGRGTAGGAEINNRLDTVSPPAVVA
                     SAPVGAASGAASSAASAAVVVPASAPSGVQGVPTR"
     gene            6663..6747
                     /locus_tag="VIN75_17985"
     tRNA            6663..6747
                     /locus_tag="VIN75_17985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6697..6699,aa:Leu,seq:gag)
     gene            6940..>7233
                     /gene="ndhC"
                     /locus_tag="VIN75_17990"
     CDS             6940..>7233
                     /gene="ndhC"
                     /locus_tag="VIN75_17990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8976111.1"
                     /translation="MDLTAYLPVILFILVGVGIGVAPQLLGFLLGPKRPDAQKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALKDIGHAGFWAMMI"
BASE COUNT         1178 a         2496 c         2481 g         1078 t
ORIGIN      
        1 tcgcgctgcc gcgtccgctg ctgcacagcg gcgacctcga cttctcgttc gccggattga
       61 agacggccgt gatgaccgcg atgcgcaagt tcggcccgga gccgaccgac cggcagcgcg
      121 ccgacctggc cgccagcacg caggcggcca tcgtagaggt gctcgtgaag aagtcgctgc
      181 gcgcgctgca ggagacgcgg ctgtcgcgcc tggtcgtggc cggcggcgtc ggcgccaaca
      241 cgcggctgcg cgagcacctg gacggcgtcg cggcgcggcg cggcctgcgc gtgcactacc
      301 ccgagctcgc gctgtgcacc gacaacggcg ccatgatcgc gctggccggc gcactgcgcc
      361 tgcagcgcga tccgtcgcag gccaggcggg actatgcgtt cgacgtgaag ccgcgctggc
      421 cgctggcgca ggcgtgagct gatgcggggc aggcctacga agcctgcctt gcgcggacga
      481 aaaaaagtcc ttcgcgccca caggcgggaa ggactcgcgc agcgcccgcg gtggtgtggc
      541 gctgcaagac cgggcggcgg gcgcccggcg cttcgaggga cttcagttga cggtcagttg
      601 cgtcgtcgcg gggctgcgct tggccagcgt cagcgtcagc acgccgttgt ccagcttggc
      661 ctgcgacgcg gcctggtcga tctcggactg cagcgtgaag ctgcgcgcga agctggcggt
      721 ggcgcgctcg cgcaggatca cgcgctcgcc ggccttgggc gcttcggcag tcgtgtcggt
      781 gggcgcgtcg gcggccggcg tctcggcgcg ggcctcggcg acgatgctca cggcgcggcc
      841 gtcgatcgac accttgacgt cctcgcgggc gacgcccggc acgtccagcg tcacgacata
      901 ggcgctgtcg gactcggcga cgtcgatggc ggggcggcgc accggcgcgg catcggcggc
      961 gggctgggcg aagatgtggc cgaaggcgtc gtcgaacagg cggtcgaaat gacgggcacg
     1021 gacggcggcg cggcgggaga cgggaacgat gaacatggcg ggactcctgg atggattcaa
     1081 agggttcttc gacgtcatgc tcgccgccgg ttggcgcgat tcagcgtcgc atgccacccc
     1141 agttggaatc gtccggaatc atttcaagag acgatatttt tcgcgggcgg ccattgaaat
     1201 ccgcacggtc gcaccgacga tccgccgcgg ccgggcaggg cgccggcggc cgcggacgag
     1261 cgacgtccag gccgagcatg gcggcggcga tcgctttcgg ctataatccg ccgcttgtcc
     1321 tcggtgtcgg cttccatgtg tggaaacgtc gccaggggca gatccagcac gccccgggtc
     1381 ggtttcactc gggtgcgcaa gtcgtcaagg aaaccgagca ggccccgccg ccccttgcgg
     1441 acgcggcgtg gctgcgaatc cgggtccatg cccggtcagc gctgccggtt tccgttcaac
     1501 atgaaggaaa tccgcaatga ctgacatcaa caaggcagag atcgtcgccg cccacgcgcg
     1561 cggtgccaac gacaccggct ccccggaagt ccaggtcgcc ctgctgaccg cccgcatcaa
     1621 cgcgctgacc ccgcacttca agcagcacgc caaggaccac cacggccgcc gcggcctgct
     1681 caagatggtc aactcgcgca agagcctgct ggcctacctg aagaagacgg acgccaaccg
     1741 ctacctcgcc ctgatcggca agctcggcct gcgcaagtaa gcatcgatgc atgaaaagag
     1801 cctgtctgga accctccagg acaggctctt tgcgtttgga gcgcgacccg ttgcgttgcc
     1861 ggccgatgtg tcattccacc gatgttcgga gcacgaatct cgctggaatg gcatcgcgcc
     1921 aatgaacgga agcctcccgt ctcccgcgcc acgacacggc gcaccataaa catcggagaa
     1981 agaacaacat gagcatgttc aacaaggtca ccaagacatt ccagtggggc cagcacaccg
     2041 ttcgcctcga gaccggcgag atcgcccgcc aatccaccgg cgccgtcgtc gtcgacatcg
     2101 aaggcaccgt cgtgctcgcc accgtcgtgg ccaagcgcga agccaagccg ggccaggact
     2161 tcttcccgct gacggtcgac tacatcgaga agacgtatgc cgccggcaag atccccggca
     2221 gcttcttcaa gcgcgaaggc cgcccgagcg agcacgagac gctcacctcg cgcctgatcg
     2281 accgcccgat ccgcccgctg ttcccggaag gcttcttcaa cgaagtgcag gtcgtcatcc
     2341 acgtgatgtc gctgaacccg gaagtcgacg ccgacatcgc cgcgatgatc ggctcgtcgg
     2401 ccgcgctggc gatctccggc atcccgttcg ccggcccgat cggcgccgcg cgcgtgggct
     2461 acgtcaacgg cgaatacgtc ctgaacccgg gcaagaccca gcgcatcgag tccaagctcg
     2521 acctggtcgt cgccggcacc gaggccgccg tgctgatggt cgagtccgaa gccgaccagt
     2581 tgcccgaaga cgtgatgctg ggcgccgtgg tgttcggcca cgaccagggc aaggtcgcca
     2641 tcaacgccat ccatgaactc gtgcgcgacg ccggcaagcc gctgtggctc gagtccggcg
     2701 aatggaagca gccggccaag gacgaggcgc tgatcgcgcg cgtcaccgag ctggcccagg
     2761 ccaagatcga ggcgcctacc agatccgcag caagcaggcc cgcacccagg ccctgcgcac
     2821 ggccacggcc gaagtgcagg ccgcgctggc cgaaggcgag accaaggtcg atccggtcaa
     2881 ggtcgacgcg ctgctgttcg acatcgaggc gaagatcgtc cgcggccaga tcctggccgg
     2941 cgagccgcgc atcgacggcc gcgacacccg caccgtgcgc gccatcgaga tccgcaccgg
     3001 cgtgctgccg cgcgcccacg gctcggccat cttcacccgc ggcgaaacgc aggccgtcgt
     3061 cgccacgacg ctgggcaccg agcgcgactc gcagaccatc gacgcgctgt cgggcgagta
     3121 cgccgagcgc ttcatgctcc actacaacat gcccccgttc gccaccggcg agacgggccg
     3181 cgtcggctcc cccaagcgcc gcgagatcgg ccacggccgc ctggccaagc gcgcgctggt
     3241 cgccgtgctg ccgtccaagg aagagttccc gtactcggtg cgcgtcgtct cggagatcac
     3301 cgagtcgaac ggctcgtcgt cgatggcttc ggtctgcggc ggctgcctgt cgctgatgga
     3361 tgccggcgtg ccgctgaagg cgcacgtggc cggcatcgcc atgggcctga tcaaggaagg
     3421 ctcgcgcttt gccgtgctga ccgacatcct gggtgacgag gaccacctcg gcgacatgga
     3481 cttcaaggtg gccggcacca cgcagggcat caccgcgctg cagatggaca tcaagatcca
     3541 gggcatcacc aaggagatca tgcaggtcgc cctggcgcag gccaaggagg cgcgcctgca
     3601 cattctgggc aagatggtcg agtccgtcgc cggcgccaac accgaggtgt cgcagttcgc
     3661 gccgcgcctg tacgtgatga agatcaaccc cgagaagatc cgcgacgtga tcggcaaggg
     3721 cggcgccacc atccgcgcgc tgaccgagga gacgggcacg cagatcgaca tcgccgaaga
     3781 cggcacgatc acgatcgcgt cgaccgacgc cgatcgcgcg gcgcaggcca agaagcgcat
     3841 cgaggagatc accgccgaag tcgaggtcgg caagatctac gaaggcccga tcaccaagat
     3901 cctggacttc ggcgcgctgg tcaacctgct cccgggcaag gacggcctgc tgcacatcag
     3961 ccagatcgcg cacgagcgcg tcgagaaggt caccgactac ctcaaggaag gccaggtcgt
     4021 gaaggtcaag gtgctcgaga ccgacgagaa gggccgcgtc aagctgtcga tgaaggcgct
     4081 gctggatcgc gagccgcaag gcgatcgcga gccgcgcgag taccgcgagc cccgcgaatc
     4141 gcgcgagccc cgcgaaccgc gcgacccgca gtaagcggcc agcgagcccg cccgcgctgc
     4201 ccgtgagcca ggacgtcaac gcgccgacga tgcgcgccat cgagatcgcc ggagtcggcg
     4261 gtcccgaggt gctgcgcgcc gtcgtgcgcg cgcgcccggt cgcgggcgtg ggcgaggctg
     4321 tggtgcgcgt gaccgcgtcg ggcgtcaatc gccccgacgt cctgcagcgc aaggggcact
     4381 atccggtgcc cccgggcgcg tccgacatcc cgggcctgga gatcgcgggc gtcatcgacg
     4441 gcggcgatgc cgcggccctg gcggccgccg gcttcgaggt cggcgaccgc gtctgcgcgc
     4501 tggtggccgg cggcggctac gccgagtatt gcgcggcgcc ggtcggccag atgctgccgg
     4561 tgcccgcggg cttcagcgat gtcgaggccg ccgcgctgcc cgagaacttc ttcaccgtgt
     4621 ggtcgaacgt gttcgaccgc ggccgcctcg cgcccggcga gtggctgctg gtgcagggcg
     4681 gctccagcgg catcggtgtc accgcgatcc agctgggccg ggcgttcggc gcccgggtca
     4741 tcgccaccgc gggatccgac gacaagtgcg cccgctgcgt cgagctcggc gccgaccacg
     4801 cggtcaacta ccgcaccgag gacttcgccg cgcgagcgct ggagatcacg ggcggcaagg
     4861 gcgtcgacgt cgtgctggac atggtcgccg gcgactatgt cgcgcgcgag gtctcgtgcc
     4921 tcgccgagga cggccgcatc gtgctgatcg cggtgcaggg cggcacgaag tcgcagatcg
     4981 acgccggcct ggtgctgcgc aggcgcctga cgctgaccgg ctcgacgctg cgtccgcgcc
     5041 cggtcgcgtt caaggcggcc atcgcggcgg ccctgcgcga aaaggtgtgg ccgctgctcg
     5101 agacgcgcca ggtgcagccg gtcgtacagg aagtcttccc cgcggagcag gcgtggcgcg
     5161 cccatgccgc gatggaagag ggcagccacg tcggcaagct gatgctcgcg tggggaacat
     5221 gatgcgcccc gctcgctgcg ttcgctgccc ccgaggggct ggcctgcttg ctccgcagcg
     5281 gccgggcgcg cgccggccgg ttccttcgat caacaacgtc tactgatgaa caacatgcgc
     5341 aagaagctcg ttgcaggcaa ctggaagatg cacggttcgc acacggccaa cgccgagctg
     5401 ctgcaaggca tcctggccgc gcgccccttc gtgtgcgacg tggccgtgtg cgtgccgttc
     5461 ccctacctga gcgaagtggc cgtggcgttg gcgggcagcg atgtccgctg gggcgcgcag
     5521 gactgctcgc agcacgacaa gggcgcgtac acgggcgagg tctccgcgtc catgctcgcc
     5581 gagttcggct gccgctacgc gatcgtcggc cactccgagc gccgcgcgat gcacggcgag
     5641 tccgacctgc aggtggccga gaaggccaag gccgtgctcg cgcgcggcct cacgcccatc
     5701 gtctgcgtcg gcgagacgct ggcccagcgc gagcagggcg cggcgcagga gaccgtcaag
     5761 cgccagctct cggccgtcat ccacgcgctg gcgcattgcg tcggcgagat ggtggtggcc
     5821 tacgagccgg tctgggcgat cggcaccggc gtcaccgcca cgcccgggca ggcgcaggag
     5881 atgcacgcgc tgatccgcgc ccagttgcgc gcggccacgc cgcatgccga cgcgatgagg
     5941 ctgctgtacg gcggcagcat gaagcccgac aacgccgccg cgctgctcgc gcaacccgac
     6001 atcgacggcg gactgatcgg cggcgccgcg ctcaaggctg ccgacttcgt cgccatctgc
     6061 cgcgcggccg gctgacccca ctcattcgaa cgatttccct ggagctccaa agcaaatgtc
     6121 tgtctggcac aacttcctct tcatcctgca ggtcctttcg gcgatggcca tgatcggcct
     6181 cgtgctgatc cagcacggca agggcgccga catgggcgcg tccttcggca gcggcgcgtc
     6241 gggcagcctg ttcggcgcca ccggcagcgc gaacttcctg tcgcgctcca ccgcgatctg
     6301 cgcgacgatc ttcttcgcca ccacgctggg catgtcggtg ttgtccaccg gccgcggcac
     6361 cgccggcggc gccgagatca acaaccgcct ggacaccgtg tcgccgccgg ccgtggtcgc
     6421 gagcgcgccg gtgggcgcgg catcgggtgc cgcctcgtcg gcggcttcgg ccgccgtcgt
     6481 ggtgccggcc tcggccccgt cgggcgttca gggcgtgccg acgcgctgat acgacgagca
     6541 ctggaatttt ctgtagcgga tatcgttaat cgatgatttt ttcgctatag aatgtgaggc
     6601 tgttcggtga gctacaaccc tcatgcaaac cgggcagaca ggatggcctc agggccattc
     6661 aggccgacgt ggtgaaattg gtagacacgc tatcttgagg gggtagtggc gaaagctgtg
     6721 cgagttcgag tctcgccgtc ggcaccagaa ttcttgacct tcggatggtc gcgactcccc
     6781 tccagtgagc gccgcggccg acgcgccctg cgtcaatcac cagactggac ggcgaatcgt
     6841 gcgggcgcac tcgggatcag caagctgtga tctgtcgagg cgctcttttt tctgtcctcg
     6901 gacaaaacga cgcagccgga atacgacacg actctcgcca tggatctgac cgcctacctc
     6961 cccgtcatcc tgttcatcct ggtcggggtg ggcatcggcg tcgcgccgca gctgctcggc
     7021 ttcctgctcg gccccaagcg gcccgacgcg cagaagaact ctccgtacga atgcggattc
     7081 gaggccttcg aagacgcgcg catgaagttc gacgttcgct actacctcgt ggcgatcctg
     7141 ttcatcctct tcgatctcga gatcgcattc ctgttcccgt gggccgtcgc gttgaaggac
     7201 atcggccacg cgggcttctg ggcaatgatg atc
//
LOCUS       DATCRO010000657         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_491003, whole genome shotgun sequence.
ACCESSION   DATCRO010000657 DATCRO010000000
VERSION     DATCRO010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491003"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VIN75_17995"
     CDS             <1..898
                     /locus_tag="VIN75_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976112.1"
                     /translation="LHDAASWTGRHGRPFGSMVDDRWHRRLRGRNRWLRRRSTCRRTA
                     LPQSVPRAGALTCDGGSLPIPRSSHTGVPMVATSSSVRSKKPPERASAVRHERTAAPR
                     GRHPSASIDRAASTIRERIVGALDKFDAIEGEVAGLVRASVSESLAAARGAVVDVAAV
                     IREVFAGTVDAAGQSAGALRLGIQGAARGTVAGVHDAGGDLGSAVTEIVKAAIAEGHR
                     VGAEIGSVVRYALDGVSQGLAEVGKSTAHIATRAAQDALATAVGISALAAEAVRQVIL
                     GTAEGMEEVARRHRAERASDRR"
     gene            complement(822..1664)
                     /locus_tag="VIN75_18000"
     CDS             complement(822..1664)
                     /locus_tag="VIN75_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976113.1"
                     /translation="MNAALDRIAPTVLFTYPSAAVVLAEEARSGRLHAPPAEIWTGGE
                     TLTPARRRIVADTFECPVGNEYGTSEFLSLATDCGLGALHLNADWAILESVDAHGRPV
                     SHGMSGARCLLTNLANHVQPLIRYELEDRVAIHPARCACGSPMPVIEVAGRSDDVLRL
                     GAGDDHAHALSPMALTTVLEEDAGVVDFQLLQVAPRRLELRSHHAGPRAALALDRGRD
                     ALLGYLREQGALAVEVVVQAGRAPVREPSGKFKRVRALTGDRTPAPRDDAARPPPCLP
                     PSPG"
     gene            complement(1741..3180)
                     /locus_tag="VIN75_18005"
     CDS             complement(1741..3180)
                     /locus_tag="VIN75_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8976114.1"
                     /translation="MTTLLDDLRAIVGTAHVLVDDGGDALLGYERDWRKRYRGRALCV
                     VLPGSTAEVAAVVHRCAREDGVSIVAQGGNTGLVGGGVPDASRTQVVLGTRRLRAVRR
                     VDVDNQTITVEAGLALQEVQAAAADAGLLFPLSLASEGSCTIGGNLATNAGGTQVLRF
                     GNARELCLGLEVVTAQGQVWSSLHGLRKDNTGYDLRDLFVGSEGTLGIVTAATLRLFP
                     RPRAGVTAWATCRTLADAVALMRRLRADFDAELVGYEAMNAASLELVQRHFETLRAPL
                     PLTGDAGTPLWSVLVDLASPHGQEVLRERAEDTLAASLDAGETVDVAVPASQAQARAL
                     WQLREAIPLAQGVEGLNIKHDVGVPLSSLAGFVDAADAALQALVPGARHITFGHLGDG
                     NLHYNLQAPAGDDAAEFLARHEQAVNARVHDVVDAFGGTFSAEHGIGRLKRGELALRK
                     PEAALAMMKAIKAALDPQGLLNPGVLLPD"
BASE COUNT          447 a         1146 c         1195 g          434 t
ORIGIN      
        1 gctccatgat gcggcttcct ggaccggccg gcacgggcgg cctttcggat cgatggtcga
       61 cgatcggtgg caccgccgat tgagaggacg caatcgatgg ctgcgacgcc gatcgacctg
      121 ccgccgaact gcgctgcctc aatccgtgcc gcgagcgggc gcactaacgt gcgacggcgg
      181 atcgcttccg atcccccgtt cgtcacacac aggagttccc atggtcgcca cgtcttcctc
      241 cgtccgcagc aagaagcctc ccgagcgagc cagcgccgtc cgccacgagc gcaccgcggc
      301 gccgcggggg cgccatccgt cagcttcgat cgatcgcgcc gcaagcacca tccgcgaacg
      361 catcgtcggc gcgctggaca agttcgacgc gatcgagggt gaggttgccg gactggtgcg
      421 cgccagcgtc tccgaatcgc tggcggccgc tcgcggcgcc gtggtggacg tggcggccgt
      481 gatccgcgag gtgttcgccg ggacggtgga cgccgcagga cagtccgcag gcgccctgcg
      541 gctcggcatc cagggtgccg cgcgcgggac cgtggcaggc gtgcacgacg ccgggggcga
      601 cctcggcagc gccgtgacgg agatcgtcaa ggcggcgatc gccgaaggtc accgggtcgg
      661 cgccgagatc ggcagcgtcg tgcgctacgc gctggacggc gtgtcgcaag gactggccga
      721 agtgggcaag agcaccgccc acatcgcgac gcgggccgcg caggacgcgc tcgcgacggc
      781 ggtgggcatc tccgcgctcg cggccgaggc cgtgcggcag gtcatcctgg ggacggcgga
      841 aggcatggag gaggtcgcgc ggcgtcatcg cgcggagcgg gcgtccgatc gccggtgaga
      901 gcgcgcacgc gcttgaactt cccggacggc tcccggacgg gggctcgtcc cgcctgcacg
      961 accacctcga ccgcgagcgc cccttgttcg cgcaggtagc cgagcaacgc atcccgtccg
     1021 cgatccagcg ccagcgccgc ccgcgggccc gcatggtgcg atcgcaattc caggcgccgt
     1081 ggagcgacct gcagcagctg gaaatcgacc acgcccgcgt cctcctccag cacggtcgtc
     1141 aacgccatcg gagacagtgc gtgcgcgtga tcgtctcctg cgccgaggcg caggacgtca
     1201 tcgctgcggc cggccacctc gatgacgggc atcgggctgc cgcacgcgca tcgagccgga
     1261 tggatcgcga cgcggtcctc gagttcgtac cggatcagcg gctgcacgtg gttggcgagg
     1321 ttggtcagga ggcagcgcgc cccgctcatg ccatgcgaaa cgggacgccc gtgggcatcg
     1381 acgctctcca ggatggccca atcggcgttg aggtgcagtg cgcccagccc gcagtcggtg
     1441 gccaaggaca ggaattccga ggtgccgtac tcgttgccga cgggacattc gaacgtgtcg
     1501 gcgacgatgc ggcgcctagc cggcgtcagg gtctcgcccc cggtccatat ctcggccgga
     1561 ggagcgtgca ggcggccgct gcgggcctcc tcggccagga cgacggccgc ggacgggtag
     1621 gtgaacagca cggtgggcgc gatgcggtcg agggccgcgt tcaacgctgc ctgcggttgc
     1681 agcagcgaga ccgggtgcag gcgggggcga aaccacgggt tgaggcggcg cagccgggcc
     1741 tcaatcgggc agcagcacgc ccgggttgag caggccctgc ggatcgagcg cggccttgat
     1801 cgccttcatc atcgcgaggg cggcctccgg cttgcgcagc gcgagctcgc cgcgcttgag
     1861 gcggccgatg ccgtgctcgg ccgagaacgt cccgccgaag gcgtcgacga cgtcgtgcac
     1921 gcgcgcgttg acggcctgtt cgtgccgggc taggaactcg gcggcgtcgt cgcctgcggg
     1981 cgcctgcagg ttgtagtgca ggttgccgtc gcccaggtgg ccgaaggtga tgtggcgcgc
     2041 gcccggcacc agcgcctgca gcgcggcgtc ggcggcgtcg acgaagccgg ccagcgacga
     2101 cagcggcacc ccgacgtcgt gcttgatgtt cagcccctcg acgccttgcg ccagcgggat
     2161 cgcctcgcgc agttgccaca gcgcgcgggc ctgggcctgg ctggccggaa cggcgacgtc
     2221 caccgtctcg cccgcgtcga gcgacgcggc cagcgtgtcc tcggcgcgct cacgcagcac
     2281 ctcctgccca tgcggcgagg ccaggtcgac cagcaccgac cacagcggcg tgccggcatc
     2341 gcccgtcagc ggcagcgggg cgcgcagcgt ctcgaagtgg cgttggacca gctcgagcga
     2401 cgcggcattc atcgcctcgt agccgacgag ctcggcgtcg aagtccgcgc gcagtcggcg
     2461 catgagggcg accgcgtcgg ccagcgtgcg gcaggtcgcc caggcggtga cgccggcgcg
     2521 cggccgcggg aacagccgca gcgtcgcggc ggtgacgatg ccgagcgtgc cttcgctgcc
     2581 cacgaacagg tcgcgcaggt cgtagccggt gttgtccttg cgcaagccgt gcaggctgct
     2641 ccacacctgg ccctgcgcgg tcaccacctc caggcccagg cacaattcgc gcgcgttgcc
     2701 gaagcgcagc acctgcgtgc cgccggcgtt ggtggcgagg ttgccgccga tcgtgcaact
     2761 gccctccgag gccaggctca gcgggaacag caggcccgcg tcggcggccg ccgcctgcac
     2821 ctcctgcagc gcgaggccgg cctcgacggt gatcgtctgg ttgtccacat cgacgcgccg
     2881 cacggcgcgc agcctgcggg tgccgagcac gacctgcgtg cgcgaggcgt cgggcacgcc
     2941 gccgcccacc agccccgtgt tgccgccttg cgcgacgatg ctgacgccgt cctcgcgcgc
     3001 gcagcggtgc acgaccgcgg ccacttcggc ggtcgagccg ggcagcacga cgcacagcgc
     3061 gcggcctcga tagcgcttgc gccagtcgcg ctcatagccg aggagggcgt cgccgccgtc
     3121 gtcgaccagc acatgggccg tgccgacgat cgcgcgcagg tcgtccagca gcgtcgtcat
     3181 gtcaatggtc ggtgccggcg ttcgcgcgtg ggtcgtcggc gg
//
LOCUS       DATCRO010000658         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_524837, whole genome shotgun sequence.
ACCESSION   DATCRO010000658 DATCRO010000000
VERSION     DATCRO010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524837"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="kdpB"
                     /locus_tag="VIN75_18010"
     CDS             <1..1216
                     /gene="kdpB"
                     /locus_tag="VIN75_18010"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY8976115.1"
                     /translation="ANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASAFLVAQGV
                     KEAELADIAQLASLADETPEGRSIVVLAKQKFNLRERDIQALGAQFVHFSAQTRMSGV
                     DLPVAGDAPPRQIRKGAADAIRKHVESLGAKFPADLLTTIESISRRGSTPLVVSDGTR
                     VMGVIELKDIVKGGIKERFAELRRMGIKTVMVTGDNKLTAAAIAAEAGVDDFLAEATP
                     EQKLKLIRDYQAQGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSN
                     PTKLIEVVETGKQMLMTRGSLTTFSIANDVAKYFAIIPAAFVTTYPQLAALNLMHLAS
                     PSSAVLSAVIFNALVIIFLIPLALKGVKYRAVGAALLLRRNLAIYGLGGVIVPFVGIK
                     LIDLVLQVLGFV"
     gene            1230..1808
                     /gene="kdpC"
                     /locus_tag="VIN75_18015"
     CDS             1230..1808
                     /gene="kdpC"
                     /locus_tag="VIN75_18015"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEY8976116.1"
                     /translation="MKSMIRPAVSLFVLLTVVTGVIYPLAVTGVAKVAFPEQAAGSLI
                     VENGKTVGSSLIGQNFTDPKFFWGRPSATSPMAYNGQGSGGSNLGPLNPALVDAVKGR
                     VDALRAADPANKAPVPVDLVTASASGLDPEISVAAADYQVARIARLRHLSPEVVQGIV
                     ARHIHGRLLGIFGEPRVNVLDINLELRRTNAS"
     gene            1926..2783
                     /locus_tag="VIN75_18020"
                     /pseudo
     CDS             1926..2783
                     /locus_tag="VIN75_18020"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
     gene            2829..>4250
                     /locus_tag="VIN75_18025"
     CDS             2829..>4250
                     /locus_tag="VIN75_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY8976117.1"
                     /translation="MTDSRPDPDRLLAKIKDEEIQASRGKLKLFFGASAGVGKTFAML
                     SAARIQQQQGVDVVIGVIETHGRAETEALLAGLPTIALKEVRYRDRVLKEFDLDATLA
                     RKPQLVLVDELAHSNVQGSRHAKRWQDVEELLDAGIDVWSAMNVQHLESLNDIVTGIT
                     GIRVWETVPDKVFDETDEVVIVDLPPDDLLLRLKEGKVYLPQQAERAVRNFFRKGNLI
                     ALRELALRRTADRVDDDILHWRRTSQSAPVWQTREALLLCVGPDERSEKLVRTTARLS
                     AQLGVPWHCIYVETPALQRLPAETRQRILELLKSAEDNGAVTAVLSGSDLAATIVRYA
                     HEHNLPRVVLGRETGRLRGLWRRRLAEAVGALADDLDILQVALSSRDSAASRRRKTSV
                     HQKDTIPWASYGWSALICGAVTLAVAPLAQILASANLVMIFLAAVVGVAVRFGRGPAV
                     LASLLTVGMFDFFYVPPRFSFSVS"
BASE COUNT          703 a         1506 c         1384 g          657 t
ORIGIN      
        1 ggccaacgtc atcgccacgt cgggccgcgc ggtggaagcc gcgggcgacg tcgacgtgct
       61 gctgctggac aagaccggca ccatcacgct cggcaatcgc caggcgtccg ccttcctcgt
      121 ggcgcagggc gtcaaggagg ccgagctggc cgacatcgcg cagctggctt cgctggccga
      181 cgaaacgccg gaaggtcgca gcatcgtcgt gctggccaag cagaagttca acctgcgcga
      241 gcgcgacatc caggccctcg gcgcgcagtt cgtgcacttc tcggcgcaga cgcgcatgag
      301 tggcgtcgac ctgcctgtcg ccggcgacgc gccgccacgc cagatccgca agggcgcggc
      361 cgatgccatc cgcaagcacg tcgagtcgct cggcgccaag ttcccggccg atctgctgac
      421 cacgatcgag agcatctctc gccgcggcag cacgccgctg gtcgtctccg acgggacgcg
      481 cgtgatgggc gtcatcgagc tcaaggacat cgtcaagggc ggcatcaagg aacgcttcgc
      541 cgagctgcgc cgcatgggca tcaagacggt gatggtcacc ggcgacaaca agctgactgc
      601 cgccgcgatc gcggccgagg ccggcgtcga cgacttcctc gcggaagcca cgcccgagca
      661 aaagctcaag ctgatccgcg actaccaggc gcagggcaag ctggtggcaa tgacaggcga
      721 cggcaccaac gatgcgcccg cgctcgcaca agccgacgtc gccgtggcga tgaactcggg
      781 cacgcaggcc gccaaggagg ccggcaacat ggtcgacctc gactcgaacc cgaccaagct
      841 catcgaggtg gtcgagaccg gcaagcagat gctgatgacg cgcggctcgc tgacgacgtt
      901 ctccatcgcc aacgacgtgg ccaagtactt cgccatcatc ccggcggcct tcgtcacgac
      961 gtacccgcaa ctggcggcgc tgaacctcat gcatctggcc agcccgtcgt cggcagtgtt
     1021 gtcggccgtg atcttcaacg cgctggtcat catcttcctg atcccgctgg ccttgaaggg
     1081 cgtcaagtac cgcgccgtcg gcgccgcgct gctgctgcgt cgcaacctgg ccatctacgg
     1141 cctgggcggc gtgatcgtgc cgttcgtcgg gatcaagctg atcgacctgg tgctccaggt
     1201 cctcggcttc gtctgaactg gagctctcca tgaaatcaat gattcgtccc gcggtcagcc
     1261 tgttcgtgct gctgaccgtc gtcaccggcg tcatctatcc gctggcggtc accggcgtcg
     1321 ccaaggtcgc gttcccggag caggccgctg gcagcctgat cgtcgagaac ggcaagacgg
     1381 tgggctcgtc cctgatcggc cagaacttca ccgatccgaa gttcttttgg ggccgcccgt
     1441 cggccacctc gccaatggcc tacaacggcc agggctccgg cggctccaac ctgggcccgc
     1501 tgaacccggc gctcgtcgat gcggtcaagg ggcgcgtgga cgcgctgcgt gccgccgacc
     1561 ccgccaacaa ggcgccggtg ccggtcgacc tggtcacggc gtcggccagc ggcctcgacc
     1621 ccgagatcag cgtcgcggcg gccgactacc aggtcgcgcg catcgcgcgg ctgcgacacc
     1681 tgtcgcccga agtcgtccag ggcatcgtcg cgcgccacat ccatggccgc ctgctgggca
     1741 tcttcggcga gccgcgcgtc aacgtcctgg acattaacct cgagctgcgg cgcaccaacg
     1801 cgtcctgagc cttagacaga cagtccgcga ccggcccgga ctcgctgtcg acctcccggt
     1861 gtcctcggcc tccgggggcc gggcgcgcgc ggcccggcgg gccgtcgtct ctcgcaacca
     1921 caaccatgaa atcgaccctg atcctcgccg gcacctcggt gctggccttc tctaccctcg
     1981 tcaccgcacc gcaggcccag gccgcggacg cgccggccgc cacgcccttc atcacggcga
     2041 ccaccaacat caacctggtc agcgactacc gtttccgcgg catcgaccag acctggggca
     2101 agcccgccgt gcagggcggt gccgacctga cgctggccaa cggcatctac gccggcgtgt
     2161 gggcttccaa tgtctcgggc aattcgtact cgggcggcag cctcgagacc gactggtacg
     2221 ccggctacaa cggcaagttc agcgacgact tcggctacac ggtgggtggc tacggctaag
     2281 tctacccggg cgcgaacttc gacaagtcgg cctgcccgtc ggccgcctac ccggcgccgt
     2341 gcggcacgct gccgtcgcaa tccctgaaca cgttcgagct gaacgccggc gtgtcctgga
     2401 agtggatcag ttacaagctg tcagtctcga ccagcgacta cttcggcgcc aatgcctcca
     2461 ccggctattc gggcaagacc agcggcacgc tttaccacga cctgacggcc acctgggcga
     2521 tcacggacga catcagcctg gtgggccacg tcggtcacag cgacatcaag gcgacctacg
     2581 ccggcatcaa ccccgactac accgactacc gcgtgacggt cagcaagacc ttcgcgggtg
     2641 gctggagcgt gtcggccgcc ggcgccggcg cgaacaacga caagttctac cgcccgccgg
     2701 tcggcggcct gtcgttcgcc aatggccaga cgcgcaacgt caacaagccg gtgctgatcg
     2761 cgcaggtggg ccgcaccttc tgatgcgcca cgggtctgct accttcagga gttcaaggag
     2821 acctgcccat gacggatagc cgtcccgacc ccgaccgcct gctggccaag atcaaggacg
     2881 aggagatcca ggccagccgc ggcaagctca agctgttctt cggcgcgtcg gcgggcgtgg
     2941 gcaagacctt cgccatgttg tcggcggcgc gcatccagca gcaacagggc gtcgacgtcg
     3001 tcatcggcgt catcgagacc catggtcgcg cggaaacgga ggcactgctc gctggcctgc
     3061 cgacgatcgc cttgaaggag gtgcgctacc gcgaccgcgt cctgaaggag ttcgacctgg
     3121 acgcgacgct ggcgcgcaag ccgcagctgg tgctggtcga cgagctggcg cactccaatg
     3181 tgcagggttc gcgccatgcc aagcgctggc aggatgtcga ggaattgctg gacgccggca
     3241 tcgacgtctg gtcggcgatg aacgtccagc acctggagag cctgaacgac atcgtcacgg
     3301 gcatcaccgg catccgcgtc tgggaaacgg tgccagacaa ggtcttcgac gaaaccgacg
     3361 aggtcgtcat cgtcgacctg ccgccggacg acctgctgct gcgcttgaag gagggcaagg
     3421 tctacctgcc gcagcaagcc gagcgcgccg tccgcaactt ctttcgcaag ggcaacctga
     3481 tcgccctgcg cgagctggcg ctgcggcgga cggcggaccg cgtcgacgac gacatcctgc
     3541 actggcggcg cacgagccag tcggcgccgg tgtggcagac gcgcgaggcg ctgctgctgt
     3601 gcgtcggccc cgacgagcgc tccgagaaac tcgtacgaac cactgcgcga ctgtcggcgc
     3661 agttgggcgt gccctggcat tgcatctacg tcgagacacc cgccctgcag cgcctgccgg
     3721 ctgagacgcg tcaacgcatc ctcgagctgc tgaagtccgc cgaggacaac ggcgccgtca
     3781 cggccgtcct cagcggcagt gacctggccg cgaccatcgt gcgctacgcc cacgagcaca
     3841 acctgccgcg cgtggtgctc ggacgcgaga cgggccggtt gcgcggcctc tggcgacggc
     3901 ggttggccga ggcggtcggc gccttggccg acgacctgga catcctccag gtcgcgctgt
     3961 cgtcgcgcga cagcgcggcg agtcgacgcc gcaagaccag cgtccaccag aaggacacga
     4021 taccctgggc gagctacggc tggagcgcac tgatctgcgg cgcggtcacg ctggccgtcg
     4081 ccccgcttgc ccagatcctg gccagcgcta acctcgtgat gatcttcctg gccgccgtcg
     4141 tgggcgtggc cgtgcgcttc ggccgcgggc cggcggtact ggcgtcgctg ttgacggtgg
     4201 gcatgttcga cttcttctac gtgccaccgc gcttttcctt cagcgtgtcc
//
LOCUS       DATCRO010000659         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_951871, whole genome shotgun sequence.
ACCESSION   DATCRO010000659 DATCRO010000000
VERSION     DATCRO010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951871"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..462
                     /locus_tag="VIN75_18030"
     CDS             79..462
                     /locus_tag="VIN75_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976118.1"
                     /translation="MTARLAPTLVAAALLALSGTSAFAQHNPKPAPAPEAPAPAPKAS
                     VDPATGMLVDEAPPVPSKDAKVEHLVSEDSQVRIEETRVRGVTKKIVVHSKITGDSYE
                     IQPRDPSLAPEHDKGAGMRTFTFGF"
     gene            528..1496
                     /locus_tag="VIN75_18035"
     CDS             528..1496
                     /locus_tag="VIN75_18035"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY8976119.1"
                     /translation="MAVYTEVDVDTAAALLRTLDAGSLTSMRGAAGGIENTNYFVHSD
                     KGDWVLTVFERLGFDQLPFYLGLMQHLARKGLPVPEPRTDASGNFLHSIAGKPASLVN
                     LLPGQSIDAPMLAHCSLLGVTLAHLHTAAVDYPLQQPNLRGLEWWAKTVPVVQPHVGP
                     ALARRLAEELDFQQRFHAAASTQSLPKGPVHADLFRDNALFDGPPESPRLSGVFDFYF
                     AGVDHHVFDLAVCVNDWCIDLDTGRLEPEHAQALLRAYHVHRPMSPAELRALPGMRRA
                     AALRFWLSRLADWHLPREAALLKPKDPTHFERVLLDCIENPWHPEL"
     gene            1545..2444
                     /locus_tag="VIN75_18040"
     CDS             1545..2444
                     /locus_tag="VIN75_18040"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY8976120.1"
                     /translation="MTPTPSIRIRSVPALAGLRWIRLALRAFGRQPGGFMGMFGLYML
                     FLLLLTIPLSVLGPLAEAIHVDAIVVGSLTLVAMPLLSLAFMLSTEAVTNDLRISPAL
                     LFAPLRAGAPGRGALVAIGFVYFGVFLLAWFAGNGIDGGESVKWFLDHAVVSPDTGKA
                     PPAPAPLSDAGRAVLLLKMAIVALGSVPLWHAPALVQWGRYGAAKAMFASVVAMWRTR
                     GALAMFGLGWFALSFVATAAIGLLALVLGESIVLVLIAAMTYWALSALFYVTLWFGFN
                     DTFEIRSPVPFRTVMASDEPPAS"
     gene            2545..>2896
                     /locus_tag="VIN75_18045"
     CDS             2545..>2896
                     /locus_tag="VIN75_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8976121.1"
                     /translation="MKTTETIHKLNKLAHSHGPLPTGRGMGSGVVALILAILCFLGVL
                     AFHFPQYLSTPELRKSYDVSTIRLIMYWAMVVAGGISLYNILFARTRWLAASAFFLVA
                     ASALLGGAKVPVNNF"
BASE COUNT          376 a         1073 c          982 g          465 t
ORIGIN      
        1 gccgagcgcc gcggcgctgg acagcgagcc ttcggaatcc gcgaagtcca gccgggggcg
       61 agcgactaga atgagggcat gactgcccgc ctcgctccga ccctcgttgc cgccgccctg
      121 ctggcgctgt ccggcacgtc cgccttcgcg cagcacaacc cgaagccggc cccggctccg
      181 gaagcgcccg cgccggcgcc caaggcgagc gtcgatccgg ccaccggcat gctggtggac
      241 gaggcgccgc cggtgccgag caaggatgcg aaggtcgagc acctggtgtc ggaagactcg
      301 caggtgcgca tcgaggagac ccgcgtgcgc ggcgtcacca agaagatcgt ggtgcactcc
      361 aagatcaccg gcgactccta cgagatccag ccgcgcgatc ccagcctggc gccggagcac
      421 gacaagggcg ccggcatgcg cacgttcacg ttcggcttct gagccgtgca cggcgcgccc
      481 cacgcgcgcg gcgtcctggc ctgcgccgcc ctccgacgga tccgctgatg gctgtctaca
      541 ccgaagtcga cgtcgacacg gcagccgcgc tgctgcgtac gctggacgcg ggctccctca
      601 cctcgatgcg cggcgccgcc ggcggcatcg agaacaccaa ctacttcgtc cactccgaca
      661 agggcgactg ggtgctgacg gtgttcgagc gcctgggctt cgaccagctg ccgttctacc
      721 tcggcctgat gcagcacctg gcgcgcaagg gcctgccggt gcccgaaccg cgcaccgacg
      781 cgtcgggcaa tttcctgcac tcgatcgccg gcaagccggc gtcgctggtg aacctgctac
      841 ccggccagag catcgacgcg cccatgctgg cgcactgctc gctgctgggc gtcacgctcg
      901 cgcacctgca caccgcggcg gtcgactacc cgctgcagca acccaacctg cgcggcctgg
      961 agtggtgggc gaagacggtg cccgtcgtgc agccgcacgt cggccccgcg ctcgcccggc
     1021 gcctggccga ggagctcgac ttccagcagc gcttccacgc cgccgccagc acgcagtcgc
     1081 tgcccaaggg gccggtgcat gccgacctgt tccgcgacaa cgcgctgttc gacggcccgc
     1141 ccgagtcccc gcggctgtcg ggcgtgttcg acttctactt cgcgggcgtc gaccaccacg
     1201 tgttcgacct ggcggtgtgc gtcaacgact ggtgcatcga cctggacacc ggccgcctcg
     1261 agcccgaaca cgcgcaggcg ctgctgcgcg cctaccacgt gcatcggccg atgagtcccg
     1321 ccgagctgcg cgcgctgccc ggcatgcggc gcgcggcggc gctgcgcttc tggctgtcgc
     1381 gactggccga ctggcacctg ccgcgcgagg ccgcgctgct caagcccaag gatccgacgc
     1441 acttcgaacg cgtgctgctc gactgcatcg agaacccgtg gcatcccgag ctctgagatg
     1501 atgcccccgc gccggcgctg cttcgcccgg cgcccctgca cgcgatgacc ccgaccccct
     1561 ccatccgaat ccgcagcgtc cccgcgctgg ccggcctgcg ctggatccgt ctcgcgctgc
     1621 gcgcgttcgg acgccagccc ggcggcttca tggggatgtt cggcctgtac atgctgttcc
     1681 tgctgctgct gacgatcccg ctgtcggtgc tggggccgct ggccgaggcc atccacgtcg
     1741 atgcgatcgt cgtgggctcg ctgacgctgg tggcgatgcc gctgctgtcg ctcgcgttca
     1801 tgctcagcac cgaggccgtc acgaacgacc tgcgcatcag cccggcgctg ctgttcgcgc
     1861 cgctgcgcgc gggcgcgccg ggacgcggcg cgctggtggc gatcggcttc gtctacttcg
     1921 gcgtgttcct gctggcctgg ttcgcgggca acggcatcga cggcggcgag agcgtcaagt
     1981 ggttcctcga ccacgcggtg gtgtcgcccg acaccggcaa ggcgccgccc gcgccggcgc
     2041 cgctgagcga tgcgggccgc gccgtcctgc tgctgaagat ggccatcgtc gcgctcggct
     2101 cggtcccgct gtggcacgcg ccggcgctgg tgcagtgggg ccgctacggc gcggccaagg
     2161 cgatgtttgc cagcgtcgtg gcgatgtggc gcacgcgcgg cgcgctggcg atgttcgggc
     2221 tgggctggtt cgcgctgtcg ttcgtggcga ccgccgccat cggcctgctg gcgctggtgc
     2281 tgggcgaatc gatcgtgctg gtgctgatcg ccgcgatgac gtactgggcc ctgtccgcgc
     2341 tgttctacgt gacgctgtgg ttcggattca acgacacctt cgagatccgc tcgcccgtgc
     2401 cgtttcgcac ggtgatggcg tccgacgaac cgccggcgtc ctgacaaacc ctggaatccc
     2461 cccgggagag gggcgacagg cttcgcggtt tcggcttcaa tccaggctcg cgccctgcgc
     2521 gccctgcaac cgccaagcca ccggatgaag acgaccgaga cgatccacaa gctcaacaag
     2581 ctcgcccact cgcacggccc gctgcccacc gggcgcggca tgggctccgg ggtggtcgcg
     2641 ctgatcctgg ccatcctctg cttcctgggc gtgctggcgt tccacttccc gcagtacctg
     2701 tccacgccgg agctgcgcaa gtcctacgac gtgtcgacca tccggctgat catgtactgg
     2761 gcgatggtcg tggccggcgg catctcgctg tacaacatcc tgttcgcgcg cacccgctgg
     2821 ctcgccgcgt cggcgttctt cctcgtggcg gcgtcggcgc tgctgggcgg cgccaaggtg
     2881 ccggtcaaca actttc
//
LOCUS       DATCRO010000660         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_322732, whole genome shotgun sequence.
ACCESSION   DATCRO010000660 DATCRO010000000
VERSION     DATCRO010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322732"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VIN75_18050"
     CDS             complement(<1..349)
                     /locus_tag="VIN75_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976122.1"
                     /translation="MADPIAHDDFWIPEGEGKWSRPLRFLPGEAGWVELMRLAPGVRL
                     GLHRHSGLVHAYNVQGHRRLCTGEDIGPGGYVLEPAGNVDWWEATGDEPLVVFVVVMG
                     AVEYLAEDGRVLKR"
     gene            427..1881
                     /locus_tag="VIN75_18055"
     CDS             427..1881
                     /locus_tag="VIN75_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8976123.1"
                     /translation="MDPLPMLAIALPARGSRTLARSLHGQLLAAIRSGRLAPGLRLPA
                     TRALASHLGISRNSVIAAYESLAAEGHVTARTGSGHYVADAPARAPARATPVPARARE
                     RRLNAAHRGLASPDDMVDPPRAPFDFRLGVPDTAALPFDAWRRLSARALRSMARGVPG
                     YAGPAGQPALRAAIAAHASLTRAVACTADDVLVTAGAQQAFDLLARVLVTPGKTVVAV
                     EDPGYPPARAAFAAAGAAIVPVPVDDDGLRVDLLPRDANIVCVTPSHQFPLGCVLSAA
                     RRRALLAFARRRQAVVVEDDYDGEFRFEPRPLDALQTLDDDAAVSYVGTFSKSLFPAL
                     RLGYVIAPPWARAALMRARQRADWHGNLHAQDTLAAFIAEGHLARHVRRMRGVYGERR
                     RVLLAGIARDLGRWLAPVPGCAGLHVGTRAIVDLDLARVVERARAAGINVDRIGRYAL
                     GGDVADGLLFGYGAIEAAAIPRALATLKRALAQA"
     gene            complement(1887..2906)
                     /locus_tag="VIN75_18060"
     CDS             complement(1887..2906)
                     /locus_tag="VIN75_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976124.1"
                     /translation="MDDLKPLAVFAEVVDAGSMSAAARRLGMSPSAVSQTIRALEQHG
                     GVALLHRSTRKLALTEAGERYYPHCQRLLEAARAAAQSLQQSRDAPTGELRLSAPVGF
                     AIHVAPALAPLLAEAPQLRLRLLVDDAMIDLIDARIDVAIRIGRLADSNWVARRLCDF
                     DMILCAAPDYLARMGTPTTPQQLPAHQWLAFGREAPVEGPQDGFAAPGNPVLVAAARG
                     VGRGALAGSQPSTMPLDMQAADGQRQRVHVEARIASNNQLSLQQMCEHGLGIARLGHA
                     DVAASLARGALVRVLPHWRFASLPVWAVTPRRDEAEAAKVRVAVDYLRQYFAGLPARV
                     IGDAA"
     gene            3013..3669
                     /locus_tag="VIN75_18065"
     CDS             3013..3669
                     /locus_tag="VIN75_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8976125.1"
                     /translation="MKIALIGATGFVGTALVAELLQRGHQVTALARNPAKLAARPNLV
                     AKALDAYAPDAVAAAVKGHDAVISAFNPGWDDPALYDRFMKGSQAIVGGVEQSGVKRL
                     VVVGGAGSLFVAPGVQLVDTPEFASHVPPNVVPGAKAARDALTALRGNTVLDWTFLSP
                     PALLAPGERTGQYRVGGEELLMAGAAPAGISVADLAVALVDEIETPRHVRARFTVATA
                     "
     gene            complement(3744..4133)
                     /locus_tag="VIN75_18070"
     CDS             complement(3744..4133)
                     /locus_tag="VIN75_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8976126.1"
                     /translation="MRKAQQTRSLVAAWFDFLPPAEQPVAQAIHKTIRATASELTETV
                     RQGNLLLSMNGEPLLAIAPARNQVNLLIFNGSDVEATLGPLEGVGRRQRQVAFPSAAP
                     VDTARVEAVARTSVEVARRHQAHVGHH"
     gene            complement(4143..4496)
                     /locus_tag="VIN75_18075"
     CDS             complement(4143..4496)
                     /locus_tag="VIN75_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976127.1"
                     /translation="MRPVPKINELQQTPNDFLPFDAALAARPSRVSRSQVAGFSTTHM
                     QADELGIGALLRFKSMMAHEGQVVHLARLCYDKLYAYERIAAAHASSDENLRRLALEL
                     FQIYHRRAATGLPAQ"
     gene            complement(4775..5329)
                     /locus_tag="VIN75_18080"
     CDS             complement(4775..5329)
                     /locus_tag="VIN75_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEY8976128.1"
                     /translation="MENRSLLDWHSMFMGVALLAAARSKDARKRNGACIASADYKILG
                     VGYNGLPRGCDDEDPAYWADDDADPLHSRHSYIVHAEVNAILNCVVLPLTGGTIYATQ
                     FPCPRCVQSIIQVGIQRVVYLEQKPHQVASIAAANKMLQDAGIEVKALSELEAESPTW
                     SARLEQFIRDSRQPGEPPSPPGDV"
BASE COUNT          727 a         2090 c         2001 g          802 t
ORIGIN      
        1 tgcgcttgag cacgcggccg tcctcggcca ggtattcgac ggcgcccatc acgacgacga
       61 acacgaccag cggctcgtcg ccggtggcct cccaccagtc gacgttgccc gccggctcca
      121 gcacgtagcc gccggggccg atgtcctcgc ccgtgcacag gcgccggtga ccctggacgt
      181 tgtacgcgtg cacgaggccg ctgtgccggt gcagccccag gcgcacgccc ggggccaggc
      241 gcatcagttc gacccagccc gcctcgccgg gcaggaagcg cagcggccgc gaccacttgc
      301 cctcgccctc ggggatccag aagtcgtcgt gcgcgatcgg gtcggccatg gggttctcct
      361 ttgcttcgat ggggctacga tacgcaatca ggcggcactt cataaggacc acttttcatg
      421 aagtcgatgg atccacttcc gatgttggcc atcgcgttgc cggcgcgcgg ctcgcgcacg
      481 ctcgcccggt cgctgcacgg ccagttgctg gccgcgattc gctcgggccg cctggcgccc
      541 ggcctgcggt tgccggccac ccgcgcgctg gccagccacc tggggatctc gcgcaacagc
      601 gtcatcgcgg cgtacgagtc gctcgcggcc gagggccacg tcacggcgcg caccggctcg
      661 ggccactacg tcgccgacgc gcccgcccgc gcccctgcgc gcgcgacgcc cgtccccgcg
      721 cgggcgcgcg agcgccgact gaacgcggcg catcgcggac tggcctcgcc ggacgacatg
      781 gtcgacccgc cgcgcgcgcc cttcgacttc cgcctgggcg tgcccgacac cgccgccctg
      841 cccttcgacg cgtggcgccg gctgtccgcg cgcgccctgc gttcgatggc gcgcggcgtg
      901 ccgggctacg ccggccccgc cggccagccc gcgctgcgcg ccgccatcgc cgcgcacgcg
      961 tcgctcacgc gcgcggtcgc ctgcacggcc gacgacgtgc tcgtgaccgc gggcgcccag
     1021 caggccttcg acctgctcgc gcgcgtgctg gtcacgccag gcaagacggt cgtggccgtg
     1081 gaggatcccg gctacccgcc ggcgcgcgcc gccttcgccg cggccggcgc ggcgatcgtg
     1141 ccggtgcccg tcgacgacga cggcctgcgc gtggacctgc tgccgcgcga cgcgaacatc
     1201 gtctgcgtca cgccgtcgca ccagttcccg ctgggttgcg tgctgtcggc cgcgcgccgc
     1261 cgcgcactgc tggcgttcgc gcgccgccgc caggcggtgg tcgtcgagga cgactacgac
     1321 ggcgagttcc gcttcgagcc gcgcccgctc gacgcgctgc agacgctcga cgacgacgcg
     1381 gccgtgtcct acgtcggcac cttctccaag agcctgttcc ccgcgctgcg cctgggctac
     1441 gtgatcgcgc cgccatgggc gcgcgcggcg ctgatgcgcg cgcgccaacg cgccgactgg
     1501 cacggcaacc tgcacgcgca ggacacgctg gccgccttca tcgccgaggg tcacctcgcg
     1561 cgccacgtcc gccgcatgcg cggcgtctac ggcgagcgcc ggcgcgtgct gctggccggc
     1621 atcgcgcgcg acctcggccg ctggctcgcc ccggtgcccg gctgcgcggg cctgcacgtg
     1681 ggcacgcgcg cgatcgtcga cctcgatctg gcgcgggtgg tcgaacgggc ccgcgccgcg
     1741 ggcatcaacg tcgaccgcat cggccgctac gcgctgggcg gcgacgtcgc ggacggcctg
     1801 ctgttcggct acggcgcgat cgaggcggcg gcaatcccgc gcgcgctggc gaccttgaag
     1861 cgtgcgctcg cccaggcctg atcgcttcag gcggcgtcgc cgatcacgcg cgccggcagc
     1921 cccgcgaagt actggcgcag gtagtcgacc gcgacgcgca ccttcgccgc ctcggcctcg
     1981 tcgcgcctgg gcgtgacggc ccacaccggc aatgacgcga agcgccagtg cggcagcacg
     2041 cgcacgagcg ccccgcgcgc gagcgaggcg gcgacgtcgg catggcccag gcgcgcgatg
     2101 cccagcccgt gctcgcacat ctgctgcagc gacagctggt tgttgctggc gatgcgcgcc
     2161 tcgacgtgca cgcgctggcg ctggccgtcg gcggcctgca tgtccagcgg catcgtcgag
     2221 ggctgcgagc ccgcgagcgc gccgcggccc acgcccctgg cggcggccac caggaccggg
     2281 ttgcccggcg cggcgaagcc gtcctgcggg ccctcgaccg gcgcctcgcg gccgaaggcc
     2341 agccactggt gcgcgggcag ctgctgcggg gtggtgggcg tgcccatgcg cgccaggtag
     2401 tccggcgcgg cgcacaggat catgtcgaag tcgcacagcc ggcgcgcgac ccagttcgaa
     2461 tcggcgaggc ggccgatgcg gatcgccacg tcgatgcgcg cgtcgatcag gtcgatcatc
     2521 gcgtcgtcca ccagcaggcg cagccgcagc tgcggcgcct cggcgagcag cggcgccagc
     2581 gcgggcgcga cgtggatcgc gaagcccacg ggcgccgaca ggcgcagctc gccggtgggc
     2641 gcgtcgcgcg actgctgcag cgactgcgcc gccgcgcgcg ccgcctccag caggcgctgg
     2701 cagtgcgggt agtagcgctc gccggcctcg gtcagcgcga gcttgcgcgt cgaccggtgc
     2761 agcagcgcga cgccgccgtg ctgctccagc gcgcggatcg tctggctcac ggccgacggg
     2821 ctcatgccca gccggcgggc ggcggcgctc atcgagccgg cgtcgaccac ctcggcgaag
     2881 acggccagcg gcttcaggtc atccatcgat tcgtaagctg cacttcaagg tgatggcgac
     2941 tttagccgac taccgccccg attgtgaagc gggcacagtc tcgtcatccc atccacacct
     3001 ttggagaacg tcatgaagat cgccctcatc ggcgccaccg gcttcgtcgg caccgcgctc
     3061 gtcgccgaac tgctgcagcg cggccaccag gtcacggcgc tggcgcgcaa cccggccaag
     3121 ctggccgcgc gccccaacct cgtcgccaag gcgctggatg cctatgcccc cgatgcggtg
     3181 gccgccgccg tcaagggcca cgacgccgtc atctccgcct tcaacccggg ctgggacgac
     3241 cccgcgctgt acgaccgctt catgaagggc agccaggcca tcgtcggcgg cgtcgagcaa
     3301 tcgggcgtca agcgcctggt ggtggtcggc ggcgccggca gcctgttcgt ggcgccgggc
     3361 gtccagctgg tggacacccc ggagttcgcg agccacgtgc cgcccaacgt cgtccccggc
     3421 gccaaggccg cgcgcgacgc gctgaccgcc ctgcgcggca acaccgtcct cgactggacg
     3481 ttcctcagcc cgccggccct gctggccccg ggcgaacgca ccggccagta ccgcgtgggc
     3541 ggcgaggagc tgctgatggc gggcgccgcg ccggccggaa tctcggtggc cgacctggcc
     3601 gtggccctcg tcgacgagat cgagacgccg cgccacgtgc gcgcccgctt cacggtcgcg
     3661 acggcctgat ccctgcgccc ctgaaggcaa gagaggcggc gacgcgcgcg cgtcccgcct
     3721 ccctggtccg tgccgtcgcg ggctcagtgg tggccgacgt gcgcctggtg ccggcgcgcg
     3781 acctcgacgc tggtgcgcgc caccgcctcg acgcgcgcgg tgtccaccgg tgcggcgctc
     3841 gggaacgcca cctggcgctg gcgccggccg acgccttcga gggggcccag cgtcgcctcg
     3901 acgtcgctgc cgttgaagat caggagattg acctggttgc gcgcgggcgc gatcgccagc
     3961 aggggctcgc cgttcatcga cagcagcagg ttgccctggc gcacggtctc ggtgagttcg
     4021 gacgcggtgg cgcggatcgt cttgtggatc gcctgcgcca cgggctgttc ggccggcggc
     4081 aggaagtcga accacgccgc caccagcgag cgggtctgct gggctttacg catgggaagg
     4141 ggtcactggg ccggcaggcc ggtcgcggcg cgccggtggt agatctggaa cagttccagc
     4201 gccaggcggc gcaggttctc gtcgctgctc gcgtgggcgg cggcgatccg ttcgtaggcg
     4261 tacagcttgt cgtagcacag ccgcgccaga tgcacgacct ggccctcgtg cgccatcatc
     4321 gacttgaagc gcagcagcgc gccgatgccc agctcgtcgg cctgcatgtg cgtggtgctg
     4381 aagccggcca cctgcgagcg cgagacgcgc gacggtcgcg cggccagcgc cgcgtcgaac
     4441 ggcaggaagt cgttcggcgt ctgctggagc tcgttgatct tgggcactgg gcgcatggcg
     4501 gtccacctcc gggttgcgga cgtccggcga atctggactc cacgactcga tggtgcggga
     4561 acctcccggc cgccgatcca cgaaaaagcc gcacggatgc ggcttattca acgttccaaa
     4621 tgatgcgggg tctgatgcgg ggtcaggtct tgcatgcaac tccgcgcgac gcaaccgtca
     4681 gcaaggagcc ttccggagtc gcatgcgaga gctgaccccg cctgactgga cgtggcattt
     4741 gcatgcaaga ccgcctgacc ggcgcgcgcc caggctacac atcccccggc ggcgacggcg
     4801 gctcgcccgg ctgccggctg tcgcggatga actgctcgag gcgagccgac caggtcgggg
     4861 attcggcctc cagctcggac agcgccttca cctcgatgcc cgcgtcctgc agcatcttgt
     4921 tggcggcggc gatcgaggcc acctggtgcg gtttctgctc gaggtagacc acgcgctgga
     4981 tgcccacctg gatgatggac tgcacgcagc gcgggcacgg gaactgggtg gcgtagatgg
     5041 tgccgccggt cagcggcagc acgacgcagt tgaggatcgc gttgacctcg gcgtgcacga
     5101 tgtagctgtg gcgcgagtgc agcgggtcgg cgtcgtcgtc ggcccagtac gccggatcct
     5161 cgtcgtcgca gccgcgcggc aggccgttgt agccgacgcc caggatcttg tagtcggcgc
     5221 tcgcgatgca cgcgccgttg cgcttgcgcg cgtccttgga gcgggccgcc gccagcagcg
     5281 cgacgcccat gaacatcgag tgccaatcca gcagggatct gttttccata cctgattcgt
     5341 tgtattgcgt gcgttcggag cgagtttagg gcgaacccgg ccatggcagt cccgtccgca
     5401 tcggcgagaa tcgcattgtg gtcgagcagg cccggtccgg tttttgacag ccggcatgac
     5461 gcgtgctcca ctacgacaag cgcaggcgga ccatcggcgc gcccctcgcg gacgcccgcc
     5521 gaaccgccat tcctgcagga gattccagtc atgcccagcc cgtttcgacc ggccaccgaa
     5581 ctgatggtgc aatacgcggc ctaccaccgc gaccgccgca
//
LOCUS       DATCRO010000661         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_52495, whole genome shotgun sequence.
ACCESSION   DATCRO010000661 DATCRO010000000
VERSION     DATCRO010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52495"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="tsaB"
                     /locus_tag="VIN75_18085"
     CDS             <1..507
                     /gene="tsaB"
                     /locus_tag="VIN75_18085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8976129.1"
                     /translation="VAQGLAFGQDVPVVPVDSLAIVAEDAARRLGLAAGAVVWVAMDA
                     RMDEVYAGCYRDDGDAGWRAVIEPALTTPDALHAQWRDAPPAVVAGSALDAFGDRLQT
                     GAAQRMPYEHGRAAALGWLAAAGFARGDAVPADQALPIYLRDKVAQTTAEREAAKAAR
                     EAAAGAPR"
     gene            513..1007
                     /gene="rimI"
                     /locus_tag="VIN75_18090"
     CDS             513..1007
                     /gene="rimI"
                     /locus_tag="VIN75_18090"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8976130.1"
                     /translation="MRSEVASPRREPVLRVMTVPSLDAVLAIEVQAYTFPWTRGNFID
                     SLAAGYLARVLLAEDGELIGYFVAMQGFEEMHLLNLTVAAHHQGQGHARRLLAELYTL
                     SASCSATAVWLEVRESNDRARALYGREGFVEAGRRRDYYPAPLGRREDALLMTRGVPR
                     GGSA"
     gene            1004..1933
                     /locus_tag="VIN75_18095"
     CDS             1004..1933
                     /locus_tag="VIN75_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY8976131.1"
                     /translation="MSWNERQRRMLAAMGVRLFAPAAAPSAAAAPSLAAVAAAAEAVE
                     PVVAGGTAAIEHPPEATAPAEVPRAPRAAPTPVRPPAAARGATPVAAAVEAAIDPALA
                     GRAAHIATLAWGPLHEAVAACTACGLCESRKQTVFGVGHAQADWMIVGEAPGEQEDLQ
                     GEPFVGAAGQLLDQMLRALGLSRRAESGEGVEDDMAGDPARRVFIANTLKCRPPRNRN
                     PEPEEMARCEPFLKRQVELVKPKLILLMGRFAVTQLLKSDEAIGKLRGRVHRYEGIPV
                     IVTYHPAYLLRNLPEKAKAWEDLCLAKATADGL"
BASE COUNT          258 a          752 c          781 g          246 t
ORIGIN      
        1 gtcgcccagg gcctggcctt cgggcaggac gtgccggtgg tgccggtcga cagcctggcc
       61 atcgtcgccg aggacgccgc gcgccgcctg ggcctggccg ccggcgccgt cgtgtgggtg
      121 gcgatggacg cacgcatgga cgaggtctac gccggctgct accgcgacga cggcgacgcc
      181 ggctggcgcg ccgtgatcga gccggcgctc acgacgcccg acgcgttgca cgcgcagtgg
      241 cgcgacgcgc cgccggcggt cgtcgcgggt tccgcgctgg acgcctttgg cgaccgcctg
      301 cagacgggag ccgcgcagcg catgccgtac gagcacggcc gcgccgccgc gctcggctgg
      361 ctcgccgccg ccggcttcgc ccgcggcgac gccgtgccgg ccgaccaggc gctgcccatc
      421 tacctgcgcg acaaggtcgc ccagacgacg gccgagcgcg aggccgccaa ggccgcgcgc
      481 gaggcggccg cgggggcacc acggtgagcg cggtgcgttc cgaagtcgcg tcgccgcgcc
      541 gcgagccggt gctgcgcgtg atgaccgtgc cgtcgctcga cgccgtgctg gcgatcgagg
      601 tgcaggccta caccttccca tggacgcgcg gcaacttcat cgattcgctc gcggcgggat
      661 acctggcgcg ggtgctgctg gccgaggacg gcgagctgat cggctacttc gtcgcgatgc
      721 agggcttcga ggagatgcac ctgctgaacc tgacggtggc cgcgcaccac caggggcagg
      781 gccacgcccg ccggctgctg gccgagctct acacgctcag cgcgagctgc tcggccaccg
      841 cggtgtggct cgaggtgcgc gagagcaacg accgggcccg cgcgctgtac ggccgcgagg
      901 gcttcgtgga ggccggccgc cgccgcgact actatccggc gcccctgggc cgccgcgagg
      961 acgcgctgct gatgacgcgc ggcgtcccgc gggggggcag cgcgtgagct ggaacgaacg
     1021 ccaacgcagg atgctggccg cgatgggcgt gcgcctgttc gcgcccgcgg cggctccctc
     1081 cgcggccgcc gcgccgtccc tggcggccgt agccgcggcg gccgaggccg tcgagcccgt
     1141 cgtggccggc ggcacggcgg ccatcgagca cccgccggag gcgaccgcgc ctgccgaagt
     1201 gccgcgcgcg ccccgggccg cccccacgcc cgtgcgcccg cccgccgccg cgcgcggggc
     1261 gacgccggtg gccgcggccg tcgaggccgc gatcgatccc gcgctggccg ggcgcgccgc
     1321 gcacatcgcg acgctggcct ggggtcctct gcacgaggcg gtcgccgcct gcaccgcctg
     1381 cggcctgtgc gagagccgca agcagacggt gttcggagtg ggccacgcgc aggccgactg
     1441 gatgatcgtg ggcgaggcgc ccggcgagca ggaagacctg cagggcgagc ccttcgtcgg
     1501 cgccgcgggc cagttgctgg accagatgct gcgcgcgctg ggcctgtcgc gccgggccga
     1561 atcgggcgag ggcgtcgagg acgacatggc gggcgatccg gcgcgccgcg tcttcatcgc
     1621 caacacgctc aagtgccgcc cgccgcgcaa ccgcaatccc gagccggagg agatggcgcg
     1681 ctgcgaacct ttcctcaagc gccaggtgga gctggtcaag cccaagctga tcctgctgat
     1741 gggccgcttc gcggtgacgc agctgctcaa gtccgatgaa gcgatcggca agctgcgcgg
     1801 ccgcgtccac cgctacgagg gcatccccgt catcgtcacg taccacccgg cctacctgct
     1861 gcgcaacctg ccggagaagg cgaaggcgtg ggaggacctc tgtctggcga aggcgacggc
     1921 ggacggtttg tagccagagg ggtcaggccc ttgcctccag atcgcgatcc gaaccgaggc
     1981 ttgcggcggc atcgtggcgc gatcaggagc agaggccaga cccctgcggt gcacccg
//
LOCUS       DATCRO010000662         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_783652, whole genome shotgun sequence.
ACCESSION   DATCRO010000662 DATCRO010000000
VERSION     DATCRO010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783652"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1427)
                     /locus_tag="VIN75_18100"
     CDS             complement(99..1427)
                     /locus_tag="VIN75_18100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8976132.1"
                     /translation="MHASVLRRLLIVLLALTGIALAWNQFGMTSTLVIDARSPYAVAA
                     LDDRENPGGRSVATVAHEGGAIGMDCDLRAGYEWAFCELRIKFGTGPAGLDLSRYESM
                     RLWIDATGPEAQQRVRIYVRNYDAAYSRPGVRESEKVNQLIYAPAEYPKGYEVPLARF
                     QVAQWWIDEHPMTLDLEAPDFRNATELTFSTGPKVEPGPHTIRVRRIELTGKLIPAST
                     FRLGIIAAWMLSVFGYLVVDSVLNRRQLRLSAASQHSLQRLNESLRLETRSLAEIART
                     DPLTGALNRKGLADELMRIVRMGEDKAFPMTLVFIDIDHFKRINDSLGHDTGDQVIRG
                     LAELVRSAVQRDDLFARWGGEEFLLVFRDTPGLKGSAIAERLRERIAGATWPGDLRVT
                     CSFGVAEWHRDEDLNEGVKRADEAMYRAKQQGRDRVEIELEIDAAESQLA"
     gene            complement(1613..2539)
                     /locus_tag="VIN75_18105"
     CDS             complement(1613..2539)
                     /locus_tag="VIN75_18105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY8976133.1"
                     /translation="MSRLETVLAQVTGELMRERRTERRWRLFGRAAWFLLALATVWGL
                     VASQRGKASTPNGPHTALVEVRGEIASEGNASAENIVSALRSAFEEKNAVAVVIRFNS
                     PGGSPVQAGIVYDEIKRLKALHKKKIYAVCEEMCASGAYYMAVGADEIFVDKASIVGS
                     IGVLMDGFGFTGAMDKLGIERRLITAGANKGMLDPFSPLKPEQRALAQQMIDEVHQQF
                     IAIVREGRGARLHETPDTFSGLFWNGEDAIKQGLADHYGSLDSVARDVVKAEDVIDYT
                     PTENVAERLAKRFGASIGAGAVHELETPILRR"
BASE COUNT          364 a          895 c          913 g          400 t
ORIGIN      
        1 ggcgcaaccc gccgctacgt ggcgctctgg cccggcattt ccgcgataac cgatctgcac
       61 gccgacgaac ggcgggttac gcctgcggct aacccgccct acgcgagctg cgattcggca
      121 gcgtcgatct cgagctcgat ctcgacgcgg tcgcgaccct gctgcttggc ccggtacatc
      181 gcctcgtcgg cgcgcttgac gccctcgttc agatcctcgt cgcgatgcca ctcggccacg
      241 ccgaagctgc aggtcacgcg cagatccccg ggccacgtgg cgccggcgat gcgttcgcgc
      301 aggcgttccg cgatggcact gcccttcagg cccggggtgt cgcggaagac gagcaggaac
      361 tcctcgccgc cccagcgcgc gaacaggtcg tcgcgctgca cggccgaacg caccagctcg
      421 gccaggccgc ggatcacctg gtcgccggtg tcgtggccga ggctgtcgtt gatgcgcttg
      481 aagtggtcga tgtcgatgaa gaccagcgtc atcgggaacg ccttgtcctc gcccatgcgc
      541 acgatgcgca tcagttcgtc ggccaggccc ttgcggttga gcgcgccggt cagcggatcg
      601 gtgcgcgcga tctcggccag gctgcgcgtc tccagccgca gcgattcgtt caggcgctgc
      661 agcgagtgct ggctcgcggc ggacagccgc agctgacggc gattgagcac gctgtcgacg
      721 acgaggtagc cgaacaccga cagcatccac gccgcgatga tgcccaggcg gaaggtggac
      781 gccgggatca gcttgccggt gagctcgatg cgccggacgc ggatcgtgtg cgggcccggc
      841 tcgaccttcg ggccggtgga gaacgtgagc tcggtcgcgt tgcggaagtc cggcgcctcc
      901 aggtcgagcg tcatcggatg ctcgtcgatc caccactgcg cgacctggaa gcgggcgagc
      961 ggcacctcgt agcccttggg gtactccgcg ggcgcgtaga tcagctggtt gaccttctcg
     1021 gactcgcgca cgcccggcct ggagtacgcg gcgtcgtagt tgcggacgta gatgcgcacg
     1081 cgctgctgcg cctcggggcc ggtggcgtcg atccacagcc gcatcgactc gtagcggctc
     1141 aggtccaggc cggccgggcc cgtgccgaac ttgatgcgca gctcgcagaa cgcccattcg
     1201 tagccggcgc gcaggtcgca gtccatgccg atggcgccgc cctcgtgcgc gacggtggcc
     1261 acgctgcggc cgcccgggtt ctcgcggtcg tccagcgccg cgaccgcgta cggcgagcgt
     1321 gcgtcgatca ccagcgtcga ggtcatgccg aactggttcc acgccagcgc gatgccggtg
     1381 agggcgagca ggacgatcag caggcgacgg aggacgctgg cgtgcatggc gggaaaggcg
     1441 cggcggacag gcgggtgtca gacggacatc ggcgattgcg ccgccgtctt gaggccgctc
     1501 cgcaggacgt tacacgcgca cggcccgggc ggggtgcccg ggcagccctc gaactcgggg
     1561 caagcgcgaa atgttgacgc gaaaggccgg gtcgcggcgc ggcaacgggc gctcagcgcc
     1621 gcaggatggg cgtctccagc tcgtgcacgg cgcccgcgcc gatcgaggcg ccgaagcgct
     1681 tggcgaggcg ctcggccacg ttctccgtgg gcgtgtagtc gatcacgtcc tcggccttga
     1741 ccacgtcgcg ggcgacggag tcgaggctgc cgtagtggtc ggccaggcct tgcttgatgg
     1801 cgtcctcgcc gttccagaac aggcccgaga aggtgtcggg cgtctcgtgc aggcgcgcgc
     1861 cgcggccctc gcgcacgatg gcgatgaact gctggtgcac ctcgtcgatc atctgctggg
     1921 ccagcgcgcg ctgctccggc ttgagcggcg agaacgggtc gagcatgccc ttgttggcgc
     1981 ccgccgtgat caggcggcgc tcgatgccca gcttgtccat cgcgccggtg aagccgaagc
     2041 cgtccatcag cacgccgatc gagccgacga tgctggcctt gtcgacgaag atctcgtcgg
     2101 cgcccacggc catgtagtag gcccccgacg cgcacatctc ctcgcacacc gcgtagatct
     2161 tcttcttgtg cagggccttc aggcgcttga tctcgtcgta gacgatgccg gcctgcacgg
     2221 ggctgccgcc gggcgagttg aagcggatca ccaccgcgac ggcgttcttc tcctcgaagg
     2281 cgctgcgcag cgcggacacg atgttctcgg cactggcgtt gccttccgag gcgatctcgc
     2341 cgcgcacctc gaccagcgcc gtgtgcgggc cgttgggcgt gctggccttg ccgcgctgcg
     2401 acgccaccag accccacacc gtcgccagcg ccagcaggaa ccacgccgcg cggccgaaca
     2461 ggcgccagcg gcgctccgtg cggcgctcgc gcatcagctc gccggtgacc tgcgccagca
     2521 ccgtctccag tcgcgacagg tcggccgcga cgttcagcgt cgtggtggac gg
//
LOCUS       DATCRO010000663         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_176153, whole genome shotgun sequence.
ACCESSION   DATCRO010000663 DATCRO010000000
VERSION     DATCRO010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176153"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..930
                     /locus_tag="VIN75_18110"
     CDS             142..930
                     /locus_tag="VIN75_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8976134.1"
                     /translation="MTLQRRPLLALAFAGAFLAAAARAESALDAISAKKSIAIAIPTD
                     FPPYGFVGTDMAPQGLDVDMARLIGAKLGAAVELVPVTSANRIPYLQTHKADLVISTL
                     GKNPEREKVIDFSAAYSPFFQAVFAPKGMPLKSPADLAGKTIGVTRGAIEDMELSKIA
                     PAGADIKRFEDNNATVSAFVSGQVQVIATGASVAGNMMARNPQLGTEFKLLLKDSPNF
                     IGVAKGDDKLRLKVNEIIAAAKASGEIDKLCVRWLGRPAGDLPN"
     gene            946..1623
                     /locus_tag="VIN75_18115"
     CDS             946..1623
                     /locus_tag="VIN75_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8976135.1"
                     /translation="MVQLDFAAVLADWPLLLRGAALTLALTAVATVLGVALGIACARA
                     RTHGPAAVRALAAAYVELIRNTPFIVQLFFIFFGLPSLGVRLSSEAASVIAMTLNLGA
                     YAAEIVRAGIEATPRGQLEAARSLAMTERQVFLRVVLPPSLGRVWPAMVSQIVIVMLG
                     SAVCGQIAAQELSYATNLIMSRNFRSFEANFVAAGLYLAMAVLLRQALAWAGPRWLFG
                     RIAPARR"
     gene            1625..2275
                     /locus_tag="VIN75_18120"
     CDS             1625..2275
                     /locus_tag="VIN75_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8976136.1"
                     /translation="MVEFSVWQILANLLLAARWTVALSLIAFVGGGVVGLALLVARLR
                     GGPGVAPAVSAYVQVFQGTPLLIQLFLAYFGLGLFGINVSPWIAAGGALTLYASAYLA
                     EIWRGCVDAIPSGQWEAADSLALSFGEKLRHVIAPQALRVAVAPTVGFLVQVIKGTAL
                     ASIIGFVELTKTGTMITNATFRPFTVYACVALMYFALCWPVSAFSKRLERKMRRDR"
     gene            2265..3014
                     /locus_tag="VIN75_18125"
     CDS             2265..3014
                     /locus_tag="VIN75_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8976137.1"
                     /translation="MTVEAPFSIVDIRQLRKRYGDNEVLKGVDLDVRRGEVIAIIGKS
                     GSGKSTLLRCINGLEEFQDGMLHVDGQPLKHGDAKAMRELRQHVGMIFQGFNLFPHLS
                     VGRNVMLAPTLVRKAQPQANEEAARKLLARVGLAEKFDAWPDQLSGGQQQRVAIARAL
                     AMQPSVLLCDEITSALDPELVGEVLKVVESLADEGMTLLMVTHEMNFARKVSDRVVFM
                     HAGRIHEIGPPEQIFGAPGTLELRQFLSSLH"
     gene            complement(3031..4077)
                     /locus_tag="VIN75_18130"
     CDS             complement(3031..4077)
                     /locus_tag="VIN75_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976138.1"
                     /translation="MPSRLLVGLGSGGSDTSLIQAQGLKPDFYERYLVGIDTQGGWTT
                     WNSPSGAYATYVMQQADSVGAVPMFTLFQFSADNLSDMDSLGSASYMQVYWSDLITLF
                     KAINTFGKPALINVEPDFWGFAQAVVNSNYGGDPSKAPAALASDAACAALPANATSYG
                     PCLVKLARKYAPKAAIGFPPSSWGGTSLASVVAFMNAMGANQGDLIIMQTVDRDAGCR
                     EQAALTPATAQADCVGGAGPWYWDETNQTHPNFQDNLAIGKAYHDGIGGLPIVWWQTP
                     FGVPSSTPGGTAGHYRDNKVDYFLKHPAEVVAVGGLGIVFGAGAENQTTPSTDGGQYQ
                     TLSKQYLANPAPLP"
BASE COUNT          610 a         1496 c         1470 g          660 t
ORIGIN      
        1 gagacgcctt gcagccgccg cgcgtcctgg cgccggacgc ctcgccgcgc cgcacccgcc
       61 gcctgttcac cgatctcgtt tcccccatcc cgaacccgac tgcaacgcgt gcgccgcgac
      121 ggcgccacaa ggagatctga catgaccctg caacgacgcc ccctgctcgc cctggcgttc
      181 gccggcgcct tcctcgcggc cgcggcccgc gccgagagcg cgctcgacgc gatctccgcg
      241 aagaagtcca tcgccatcgc gatcccgacc gacttcccgc cctacggctt cgtcggcacc
      301 gacatggcac cgcaaggcct ggacgtcgac atggcgcggc tcatcggcgc gaagctcggc
      361 gcggccgtgg aactggtgcc cgtgaccagc gccaaccgca tcccgtacct gcagacgcac
      421 aaggccgacc tcgtgatctc gacgctgggc aagaacccgg aacgcgagaa ggtgatcgac
      481 ttcagcgcgg cctattcgcc gttcttccag gcggtgttcg cgcccaaggg catgccgctg
      541 aagtcgccgg ccgacctggc gggcaagacg atcggcgtca cgcgcggcgc catcgaggac
      601 atggagctct cgaagatcgc gcccgccggc gcggacatca agcgcttcga ggacaacaac
      661 gccacggtgt cggccttcgt gtccggccag gtgcaggtga tcgccaccgg cgcatccgtg
      721 gccggcaaca tgatggcgcg caacccgcag ctgggcaccg agttcaagct gctgctcaag
      781 gactcgccca acttcatcgg cgtggccaag ggcgacgaca agctgcggct gaaggtcaac
      841 gagatcatcg cggcggccaa ggcgagcggc gagatcgaca agctgtgcgt caggtggctc
      901 ggccgtccgg ccggcgacct gccgaactga cgcgggagcg ttgccatggt gcagctcgac
      961 ttcgcagccg tcctcgcgga ctggccgctg ctgctgcgcg gggcggcgct gacgctggcc
     1021 ctgaccgcgg tcgccaccgt gctgggcgtc gcgctgggca tcgcctgcgc gcgggcgcgc
     1081 acgcacgggc ccgcggccgt gcgcgcgctg gcggccgcgt acgtggagct gatccgcaac
     1141 acgccgttca tcgtgcagct gttcttcatc ttcttcgggc tgccgagcct gggcgtgcgg
     1201 ttgtcgtccg aagccgcgtc cgtgatcgcg atgacgctca acctcggcgc gtacgcggcg
     1261 gagatcgtgc gcgccggcat cgaggcgacg ccgcgcggcc agctcgaggc cgcgcgcagc
     1321 ctcgcgatga cggagcgtca ggtgttcctg cgcgtggtgc tgccgccgtc gctcgggcgg
     1381 gtgtggccgg cgatggtcag ccagatcgtc atcgtcatgc tgggctcggc ggtgtgcggc
     1441 cagatcgccg cgcaggagct cagctatgcg accaacctga tcatgagccg caacttccgc
     1501 tccttcgagg ccaacttcgt cgcggccggg ctgtacctgg cgatggcggt tctgctgcgg
     1561 caggcgctcg cctgggccgg cccgcgctgg ttgttcggcc gcatcgcgcc ggcgcggagg
     1621 tgacgtggtc gagttctccg tctggcagat cctcgccaac ctgctcctgg cggcgcgctg
     1681 gaccgtcgcg ctgtcgctga tcgcgttcgt cggcggcggc gtcgtcgggc tcgcgctgct
     1741 ggtcgctcgc ctgcgcggcg gccccggcgt cgcgcccgcg gtgtctgcct atgtgcaggt
     1801 gttccagggc acgccgctgc tgatccagct gttcctggcg tacttcggcc tcggcctgtt
     1861 cggcatcaac gtctcgccgt ggatcgccgc gggcggtgcg ctgacgctgt atgccagcgc
     1921 gtacctcgcc gagatctggc gcggctgcgt cgacgccatc ccgagcggcc agtgggaggc
     1981 cgccgacagc ctcgcgctga gcttcggcga gaagctgcgc cacgtcatcg cgccgcaggc
     2041 gctgcgcgtc gccgtggcgc ccactgtcgg cttcctggtg caggtgatca agggcacggc
     2101 cctggcgtcc atcatcggct tcgtcgagct caccaagacc ggcacgatga tcaccaacgc
     2161 caccttccgc ccgttcaccg tctacgcgtg cgtggcgctg atgtacttcg cgctgtgctg
     2221 gcccgtttcc gcattcagca aacgactcga gaggaagatg cgccgtgacc gttgaagccc
     2281 cgttctccat cgtcgacatc cgccagctgc gcaagcgcta cggcgacaac gaggtgctca
     2341 agggcgtgga cctcgacgtg cgccgcggcg aggtgatcgc catcatcggc aagagcggct
     2401 cgggcaagag cacgctgctg cgctgcatca acggcctcga ggagttccag gacggcatgc
     2461 tgcacgtgga cggccagccc ctgaagcatg gcgacgcgaa ggcgatgcgc gagctgcgcc
     2521 agcacgtggg catgatcttc cagggcttca acctgttccc gcacctgagc gtcggccgca
     2581 acgtgatgct ggcgcccacg ctggtgcgca aggcgcagcc gcaggccaac gaggaggccg
     2641 cgcgcaagct cctggcgcgc gtcgggctgg ccgagaagtt cgatgcgtgg cccgaccagc
     2701 tctcaggcgg ccagcagcaa cgcgtggcga tcgcccgcgc gctcgcgatg cagccgagcg
     2761 tgctcctgtg cgacgagatc acctccgcgc tcgaccccga gctggtgggc gaggtgctca
     2821 aggtggtcga gagcctggcc gacgaaggca tgacgctgct gatggtgacc cacgagatga
     2881 acttcgcgcg caaggtcagc gaccgcgtcg tgttcatgca cgccgggcgc atccacgaga
     2941 tcggcccgcc ggagcagatc ttcggtgcgc caggcacgct cgaactccgg cagttcctct
     3001 cgagcctgca ctaggaccag gccaacccca tcacgggagc ggcgccgggt tggcgaggta
     3061 ctgcttggac agcgtctggt actggccgcc gtccgtcgac ggggtcgtct ggttctccgc
     3121 gcccgcgccg aacacgatgc ccagcccgcc gacggcaacc acctcggccg ggtgcttcag
     3181 gaagtagtcg accttgttgt cgcgatagtg gcccgccgtg ccgcccgggg tcgacgacgg
     3241 cacgccgaag ggcgtctgcc accagacgat gggaagtccg ccgatgccgt cgtggtaggc
     3301 cttcccgatc gcgaggttgt cctggaagtt cgggtgggtc tggttcgtct cgtcccagta
     3361 ccacggcccc gcgccgccga cgcagtcggc ctgcgccgtg gccggcgtca gggccgcctg
     3421 ctcacggcag ccggcgtcgc gatccacggt ctgcatgatg atcaggtcgc cctggttggc
     3481 gcccatcgcg ttcatgaagg cgaccacgct ggccagggac gtgccgcccc agctgctggg
     3541 cgggaagccg atcgccgcct tcggtgcgta cttgcgggcc agcttcacca ggcaggggcc
     3601 gtagctggtg gcgttcgccg gcagcgcggc gcaggcggcg tcgctcgcga gcgccgcggg
     3661 cgccttcgac ggatcgccgc catagttcga attgaccacc gcctgcgcga agccccagaa
     3721 gtccggctcg acgttgatga gcgccggctt gccgaaggtg ttgatggcct tgaacagcgt
     3781 gatcagatcg ctccagtaca cctgcatgta cgaggcactg cccagcgagt ccatgtcgga
     3841 caggttgtcc gcggagaact ggaacagcgt gaacatcggc acggcaccga ccgaatcggc
     3901 ctgctgcatc acgtaggtcg cgtacgcgcc cgacggcgag ttccaggtcg tccagccgcc
     3961 ctgggtgtcg atgcccacga ggtagcgctc gtagaagtcg ggcttgagtc cctgcgcctg
     4021 gatcagtgac gtgtccgagc cgcccgatcc caggcccacc agcagccggc tgggcatgcc
     4081 cagttgcgcg gccagcctgg cagccgagcc ggtgcccgcg gcggtgctgc ccgacggact
     4141 gctggccggc ggcgagccgg acgacgaacc gccaccggac gtgccgctcg acgagcctgc
     4201 cggggacgac gccgcgccgc tcgacgagct ggcggg
//
LOCUS       DATCRO010000664         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1031247, whole genome shotgun sequence.
ACCESSION   DATCRO010000664 DATCRO010000000
VERSION     DATCRO010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031247"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VIN75_18135"
     CDS             complement(<1..1806)
                     /locus_tag="VIN75_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8976139.1"
                     /translation="MGEISNNSSSAGIDLSQFYQVFFEEAGENLELMEQKLLELNVEA
                     ADDEELNAIFRCAHSVKGGAATFGFSDVAALTHQMETLLDKLRRHELNPTAAMVDVLL
                     AAGDALKAQLAVHQGSASTAPDTTELLFRIRAMAEGNAAASAPAPRAAPAPAPAVVSP
                     TLKSMPVPKAQTRAIELVVGPLEKPETADALSELFKEIEDLGTIEPLDAGRAADGMRR
                     FRVVTTTSDSELLDLFTFHVAREKIQLRSADPGFGFFHENAGAPPEENPVDPGYGFFE
                     PLPEAAAEASPAPAPAESSESAAPAAAAVAAAPRAPAKTDAKAVPSPDQATLRVSIEK
                     VDQLINLVGELVITQAMLAQNSREGQLNQQLLAGLTDLERNTRDLQEAVMSIRMIPMS
                     MVFNRFPRMLRDLAAKLDKKVELVTQGEATELDKSLVEKITDPLTHLVRNSCDHGIEL
                     PADRLAKGKPEHGTITLVASHQGGSIVIEVRDDGRGLSRQKLLAKARERGIDAPDSLS
                     DQEVYALIFAPGFSTAEQVTDVSGRGVGMDVVKKNITALGGTVEIDSAEGYGMSVKVR
                     LPLTLAIMDGMSIGVGDECYILPLASVVESFQVAPG"
     gene            complement(1866..2228)
                     /locus_tag="VIN75_18140"
     CDS             complement(1866..2228)
                     /locus_tag="VIN75_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976140.1"
                     /translation="MHTILAVDDSPSMRQMVSFTLKNAGFNVIEAVDGQDAFEKSTQR
                     DYNLVLTDQNMPRMDGISLTKKLRENPKFKQTPILILTTESSDQMKMAGRAAGATGWL
                     VKPFDPAKLIEVIGKVVR"
     gene            complement(2275..2652)
                     /locus_tag="VIN75_18145"
     CDS             complement(2275..2652)
                     /locus_tag="VIN75_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976141.1"
                     /translation="MVASHDLERLQRLLEDAGEALMGHFYGASNQMNVLRDEAPKVNL
                     DTAPIKEALQSLAGAITALQFQDMATQLIAHTQRRLRNCADRIARDAMGDDEDGEAVV
                     EDAPMRPNPVTQDEMDAGSIDLF"
     gene            2972..4147
                     /locus_tag="VIN75_18150"
     CDS             2972..4147
                     /locus_tag="VIN75_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8976142.1"
                     /translation="MPSRPAPARRTSLRVLHLEDSELDHELEMAHLRRGGTEVVSMRV
                     DSEPAFRAALDEPWDIVLSDFNVPGFSGLAALEIVKALDRPLPFILISGEIGEDVAVA
                     AMRNGASDYLLKANLARLAPAVEHAIDANEARLARERADHELALSRQRLSELAQHLQT
                     SVDNERAAIAREIHDDVGGSLTALKFDLAWIARHSKDPQVQQRAQSAVEVVTHAIESS
                     KRIMHNLHPAILEQGLVAALQWMASRFEKRTSVACSFRTSHETMQLPPGVPLVAYRAA
                     QEALTNITKHANATRVAIDLSLAGDVLSLEISDNGVGLRGADLAKARSFGIRGLHERA
                     STVGGWVDLSSSHQGTTLILSVPLSDGVGYASEFGPDAEDSRPAFEDSDDPSVWGGL"
     gene            4144..>4376
                     /locus_tag="VIN75_18155"
     CDS             4144..>4376
                     /locus_tag="VIN75_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976143.1"
                     /translation="MIRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYSELRALLR
                     NAACDVLVLDINMPGRSGLDVLAALKDDGDMKMR"
BASE COUNT          628 a         1467 c         1528 g          753 t
ORIGIN      
        1 gcccggggcg acctggaacg actcgaccac cgaggcgagc ggcaggatgt agcactcgtc
       61 gccgacgccg atggacatgc cgtccatgat ggccagcgtc agcggcaggc gcaccttgac
      121 gctcatgccg tagccttcgg ccgaatcgat ctccaccgtg ccgcccaggg cggtgatgtt
      181 cttcttgacc acgtccatgc cgacgcctcg gccggacacg tccgtcacct gttcggccgt
      241 cgagaagccc ggcgcgaaga tcagcgcgta gacctcctgg tcgctcagcg aatcgggcgc
      301 gtcgatgccc cgctcgcggg ccttggccag cagcttctgg cgcgacaggc cgcggccgtc
      361 gtcgcgcacc tcgatgacga tggagccgcc ctggtgggag gcgaccagcg tgatcgtgcc
      421 gtgctcgggc ttgcccttgg ccaggcggtc ggccggcagc tcgatgccgt ggtcgcagga
      481 gttgcgcacc aggtgggtca gcgggtcggt gatcttctcg accaggctct tgtccagttc
      541 ggtcgcctcg ccctgcgtca ccagctcgac cttcttgtcc agcttggcgg ccaggtcgcg
      601 cagcatgcgc gggaagcggt tgaacaccat cgacatcgga atcatccgga tcgacatgac
      661 cgcttcctgc aggtcgcggg tgttgcgctc gaggtcggtc aggccggcca gcagctgctg
      721 gttcagctgg ccttcgcggc tgttctgggc cagcatggcc tgcgtgatga cgagctcgcc
      781 gaccaggttg atgagctggt cgaccttctc gatggatacg cgcagcgtgg cctggtcggg
      841 cgacggcaca gccttcgcgt cggtcttggc cggcgcgcgc ggggcggcgg cgacggcagc
      901 ggcggcgggc gcggcggact cgctcgattc ggcgggcgcc ggggccggcg aggcctcggc
      961 ggcggcctcg ggcagaggct cgaagaagcc atagcccgga tcgacggggt tttcttccgg
     1021 cggggcgccg gcgttctcgt ggaagaagcc gaagccggga tcggcggagc gcagctggat
     1081 cttctcgcgc gccacatgga acgtgaacag gtccagcagt tcgctgtccg aggtggtcgt
     1141 gacgacgcgg aagcggcgca tgccgtcggc ggcgcggccg gcgtccagcg gctcgatcgt
     1201 gcccaggtcc tcgatctcct tgaacagctc gctcagcgca tcggcggtct cgggcttctc
     1261 gagcgggccg acgaccagct cgatggcgcg cgtctgcgcc ttgggcacgg gcatcgactt
     1321 gagcgtcggg ctcacgacgg ccggagccgg cgcgggcgcg gccctgggcg cgggcgcgga
     1381 ggccgcggca ttgccttcgg ccatggcgcg gatccggaac agcagttcgg tggtgtcggg
     1441 cgcggtgctg gccgacccct ggtgcacggc cagctgcgcc ttgagcgcgt cgccggcggc
     1501 cagcagcacg tcgaccatcg ccgccgtcgg gttcagctcg tggcggcgca gcttgtccag
     1561 cagcgtctcc atctggtgcg tcagcgcggc gacgtcggag aagccgaacg tcgcggcgcc
     1621 gcccttgacc gagtgcgcgc agcggaagat ggcgttcagc tcctcgtcgt cggcggcctc
     1681 gacgttgagc tccagcagct tctgctccat cagctccagg ttctcgccgg cttcctcgaa
     1741 gaagacctgg tagaactggc tgaggtcgat gcccgccgag ctgctgttgt tactgatctc
     1801 acccatgact ctgttctcct gatccggaat tctttgttgc ctgtcctcga gtgcggctca
     1861 ggaattcagc gaacgacctt gccgatgacc tcgatcagct tggcggggtc gaacggcttg
     1921 acgagccagc cggtggcgcc cgccgcgcgg ccggccatct tcatctggtc gctggactcg
     1981 gtcgtcagga tcaggatcgg agtctgcttg aacttggggt tctcgcgcag cttcttggtc
     2041 aggctgatgc cgtccatgcg cggcatgttc tggtcggtca gcacgaggtt gtagtcgcgc
     2101 tgggtggact tctcgaaggc gtcctggccg tccacggcct cgatgacgtt gaagccggcg
     2161 ttcttcaggg tgaacgaaac catctggcgc atcgaggggc tgtcgtccac ggcaagaatc
     2221 gtgtgcattg cagttctccg gaggggtctt gaattgagct tgaaggggat ggggtcagaa
     2281 caggtcgatg gagccagcgt ccatctcgtc ctgggtcacg gggttgggcc gcatcggcgc
     2341 gtcctcgacg acggcttcgc cgtcctcgtc gtcgcccatg gcgtcgcgcg cgatgcggtc
     2401 ggcgcagttg cgcagccggc gctgggtgtg cgcgatcagc tgcgtggcca tgtcctggaa
     2461 ctgcagggcg gtgatggcgc cggccaggct ctgcagcgct tccttgatgg gagcggtgtc
     2521 gagattgacc ttgggcgcct cgtcgcgcag cacgttcatc tggttggacg cgccgtagaa
     2581 gtgacccatc agcgcctcgc ccgcgtcctc gagcaggcgc tgcaggcgct cgaggtcgtg
     2641 cgaggcgacc atcaggtgaa cctgcaggtc tgccgccgcg accagcggaa ggcctccgcc
     2701 aggcggtggg gtctgggcgt cactcgtcag catcgtggct ccaagcttga tgttgtgtct
     2761 cgggacgagg cgacccttgc gcctcgaatc ccttgggatt gcgcctgatt cttcggcagg
     2821 gaccccctga acattgagga gatcacgtcg gaaatcgccg cccatttctc ggttggcggg
     2881 gactactgcc cgttccagtg cggtttttgg catcggaaaa ccccccgttt ggcagcgcgg
     2941 cggttctgaa accggcggat acactcttcg gatgccatcc cgccccgctc ctgcgcgccg
     3001 caccagcctg cgcgtgctgc acctggagga ctcggagctc gaccacgagc tcgagatggc
     3061 gcacctgcgt cggggcggca ccgaggtcgt ctccatgcgg gtcgattccg aacccgcctt
     3121 ccgggcggcg ctggacgaac cctgggacat cgtcctgtcg gacttcaacg tgcccggttt
     3181 ctcgggcctg gcggcgctcg agatcgtcaa ggcgctcgac cggccgctgc cgttcatcct
     3241 gatctccggc gagatcggcg aggacgtcgc ggtggccgcc atgcgcaacg gcgccagcga
     3301 ctacctgctc aaggccaacc tcgcgcggct ggcgcccgcc gtcgaacacg ccatcgacgc
     3361 caacgaggcg cggctcgccc gcgagcgcgc cgaccacgaa ctggccctgt cgcgccagcg
     3421 gctgtccgaa ctggcgcagc acctgcagac cagcgtcgac aacgagcgcg ccgcgatcgc
     3481 ccgcgagatc catgacgacg tcggcggctc gctcaccgcg ctgaagttcg acctcgcctg
     3541 gatcgcccgg cactcgaagg atccgcaggt gcagcagcgc gcgcagtcgg ccgtcgaagt
     3601 ggtcactcac gccatcgagt ccagcaagcg gatcatgcac aacctgcacc cggccatcct
     3661 ggagcagggg ctggtggccg cgctgcagtg gatggcctcg cgtttcgaga aacgcaccag
     3721 cgtcgcctgc agcttccgca ccagccacga gacgatgcag ctgccgccgg gcgtgccgct
     3781 ggtggcctac cgggcggcgc aggaggcgct caccaacatc accaagcacg cgaacgccac
     3841 gcgggtcgcc atcgacctgt cgctcgcggg cgacgtcctg tcgctggaga tcagcgacaa
     3901 cggcgtgggc ctgcgcggcg ccgacctggc caaggcgcgc agcttcggca tccgcggcct
     3961 gcatgaacgc gcgtccacgg tcggcggctg ggtcgacttg tccagcagcc accagggcac
     4021 gacactgatc ctgtccgtgc ccctgagcga tggcgtgggc tacgcgtcgg agttcgggcc
     4081 ggacgccgag gactcgcgac cggcgttcga agattccgac gatccatcgg tctggggggg
     4141 cctatgatcc gagtcatcct ttgcgacgac cacgcgctga tccgtcgcgg catccgcgac
     4201 acgctgtccg acgcgcccga catcgaggtg gttggcgagg ccggcgacta ctccgagctg
     4261 cgtgccctgc tgcgcaacgc cgcctgcgac gtcctcgtgc tggacatcaa catgcccggc
     4321 cgcagcggcc tggacgtcct ggccgcgctc aaggacgacg gcgacatgaa gatgcg
//
LOCUS       DATCRO010000665         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_660178, whole genome shotgun sequence.
ACCESSION   DATCRO010000665 DATCRO010000000
VERSION     DATCRO010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660178"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="gcvT"
                     /locus_tag="VIN75_18160"
     CDS             complement(<1..726)
                     /gene="gcvT"
                     /locus_tag="VIN75_18160"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796219.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8976144.1"
                     /translation="MSAETASAPLLQTPLHALHLELGARMVPFAGYSMPVQYPAGILA
                     EHRQCRHEAALFDVSHMGQILLKGDDAARALETLVPVDIVGLGLNKQRYALFTNAQGG
                     IMDDLMVTHRDDGLLVIVNAACKDADLRHLRERIGNRCDIVTMFDRGLLALQGPMAVH
                     AMARLSPGIEKLTFMTGGFFKVGGVPCYVTRSGYTGEDGFEISVAGADADTLARKLLE
                     QPEVKPVGLGARDSLRLEAGLCLY"
     regulatory      complement(776..909)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(926..1033)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1280..2386
                     /locus_tag="VIN75_18165"
     CDS             1280..2386
                     /locus_tag="VIN75_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976145.1"
                     /translation="MTQPVSRPPSAPDAEEPITEFVAHDPGVPAAPQSSIDLEIARSE
                     RASAALAATRALARAAFTDSQLAAEDAAAAAGIDAAEDAVDTEWVRTEYVALEQTLPP
                     DDDARRHGAPRNLVGGSFNPGIASQQGRTFPGASMADASMKTRTGAQARFHHEARHAP
                     RAEPAHAPTGHPARPVVSPSQAAAPSRARAPARAPARDGLRFLLAAGLGGVVVLAGGG
                     LAWRAGLLSHGAASATTAATTPPAAVAAQAEAARVLSPAAPQEIPITPAAGPTPARSQ
                     EDTDAALAAAARAAAVPAASVRAAERARVAPPQAPAAVAPSAAITPAPAVHGKANVAA
                     AIADAQAKADRFLSSNPGGTPTPAPAPTEGKPAP"
     gene            complement(2492..2980)
                     /locus_tag="VIN75_18170"
     CDS             complement(2492..2980)
                     /locus_tag="VIN75_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976146.1"
                     /translation="MSKLLAALIASLFASMAMAQASAPAMSKDVQKAESKAEKADAKA
                     ANSEAKTDAKADEKVNKAEAKASKKKTEAHNDAMKDKSKAQAKVDKADADDKAKQQAK
                     AEKTAADANADEAKADAKADKNVAKAKAEARHDKAKAAIKTEKTKAEGQADVDKAAAK
                     GN"
     gene            complement(3394..>4192)
                     /locus_tag="VIN75_18175"
     CDS             complement(3394..>4192)
                     /locus_tag="VIN75_18175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976147.1"
                     /translation="PWSTLSDKALDWLSAGLSALAFGLFFIGADTLTHGAPGPLGAIE
                     VGAGVVAGIVLFLRERHDARPLIPIDLLRIPVFALSTLTSICSFAAYTLAFIALPFWF
                     QAALHRDQVQTGLLMTPWPVGVGLVAPLAGRLADRVPAAILGTAGLLVLACGLWLMAR
                     LPSDASTLSILWRMALCGLGFGFFQAPNNRTMLGSAPRDRAGAAGGMLATARLTGMTV
                     GASVTALVLHTVAKEGEHLCLLIGVGIALAGAAVSMSRLGRSARPLP"
BASE COUNT          560 a         1491 c         1501 g          640 t
ORIGIN      
        1 gtacaggcac aggccggctt ccaggcgcag cgagtcgcgc gcgcccaggc ccacgggctt
       61 gacctcgggc tgctcgagca gcttgcgcgc cagcgtgtcg gcgtcggcgc cggccacgga
      121 gatctcgaag ccgtcctcgc cggtgtagcc cgagcgcgtg acgtagcagg gcacgccgcc
      181 gaccttgaag aagccgccgg tcatgaacgt cagcttctcg atgcccggcg acaggcgcgc
      241 catcgcgtgc acggccatcg ggccctgcag cgccagcagg ccgcggtcga acatcgtgac
      301 gatgtcgcag cggttgccga tgcgctcgcg caggtggcgc aggtcggcgt ccttgcacgc
      361 ggcgttgacg atgaccagca ggccgtcgtc gcggtgcgtg accatcaggt cgtccatgat
      421 gccgccctgg gcgttggtga acagggcgta gcgctgcttg ttcaggccca ggcccacgat
      481 gtccaccggc accagcgttt ccagcgcccg ggcggcgtcg tcgcccttga gcaggatctg
      541 gcccatgtgg gagacgtcga acagtgcggc ctcgtggcgg cactggcggt gctcggccag
      601 gatgccggcc gggtactgca ccggcatcga gtagccggcg aacggcacca tgcgggcgcc
      661 cagttcgagg tgcagggcgt gcagcggagt ctgcagcagc ggggcggagg cggtttcagc
      721 ggacattgtt gttccttcga gggcagtcgg tcccgcccca tcgtacgggc ggctcaatgc
      781 cctcgctgtc cgctttacct gagagattgc agcgccggtg tgccggcccc gcttgcccct
      841 tcggtggacg atctcgccgc cggcctcgag gggccgacga ccgcatcgtc tctctccagt
      901 gaggaacgcc tgctttcgac aggcgtctcg tcagtccttt tgcctgagcg ttcggggtgt
      961 tgcgacccct gcgccttcgg cggccggccg cgccgcgcga acgcggtagc cgactctctc
     1021 ctgacaagcg cccagtttag catcgaggag ggtggggaca aggagggtgc gcccgcccct
     1081 ccggaggggg tgcaaatatc tgttgccaac ccggcgattc catggagaat ttggcggatc
     1141 agaagaattc ccgccggcag gtcgctcggc gggaggcgca tggggggagc ggtgttggcg
     1201 tcggggtgtc cggcgagccg tcctgctcgt ttccgacccg gcagccccga tcgaatcccc
     1261 gagggtagcg agcaccgtca tgacccagcc tgtttcccgg ccgccgtcag cccccgacgc
     1321 tgaggagccg atcaccgagt tcgtcgcgca cgatccgggc gtcccggccg cgccgcagtc
     1381 gtccatcgac ctcgagatcg cgcgctccga gcgcgcctcg gccgcgctgg cggcgacgcg
     1441 cgcgctggcc cgcgccgcat tcaccgattc gcagctggcc gccgaggacg cggccgcagc
     1501 cgccggcatc gatgccgccg aggacgccgt cgacaccgaa tgggtgcgca ccgaatacgt
     1561 cgcgctcgag cagacgctgc cgccggacga cgatgcgcgc cgccacggcg cgccgcgcaa
     1621 cctggtgggc ggcagcttca accccggcat cgcctcgcag cagggccgca ccttccccgg
     1681 cgcctcgatg gccgatgcct cgatgaagac gcgcaccggc gcgcaggcgc ggttccacca
     1741 cgaagcccgg catgcgccga gggccgagcc ggcgcatgcg cccacgggtc acccggcgcg
     1801 cccggtcgtg agtccgtcgc aggccgccgc gccgtcgcgg gcgagggcgc ccgcccgggc
     1861 gcccgcgcgc gacgggctgc gattcctgct ggcggccggc ctcggcggcg tggtcgtcct
     1921 ggccggtggc ggcctggcat ggcgcgcggg gctgctgtcg catggggccg cgtccgccac
     1981 gaccgcggcg acgacgccgc ccgccgcggt ggcggcacag gccgaggcgg cgcgcgtgct
     2041 gtccccggcg gcgccgcagg agatccccat cacgcccgcc gccggcccca cacccgcgcg
     2101 cagccaggaa gacaccgacg cggcgctggc cgccgccgcc cgcgccgccg cggtgcccgc
     2161 ggcgtcggtt cgcgccgcgg agcgggcaag ggtcgcaccg ccccaagcgc ccgcggccgt
     2221 cgcgccatcg gccgccatca ccccggcgcc cgcggtgcat ggcaaggcga acgtggcggc
     2281 ggccatcgcc gatgcgcagg ccaaggcgga tcgcttcctg tcgtcgaacc ccggcggcac
     2341 gccgacgccg gcgccggctc cgaccgaagg caagcctgcg ccgtgagcgc gccgcatacc
     2401 gccacccgta tccgggttgg atcggttcaa tgagaaaagg cgccccgcaa gcgaggcgcc
     2461 tttctttcat gcgctggccg gatcggccgg atcaattgcc cttggcggcg gccttgtcga
     2521 cgtcggcctg gccttcggcc ttggtcttct cggtcttgat ggcggccttg gccttgtcgt
     2581 gacgcgcctc ggccttggcc ttggcgacgt tcttgtcggc cttcgcgtcg gccttggctt
     2641 cgtcggcgtt ggcgtcggcg gcggtcttct cggccttggc ctgctgcttg gccttgtcgt
     2701 ccgcgtcggc cttgtcgacc ttggcctggg ccttggactt gtccttcatc gcgtcgttgt
     2761 gcgcttcggt cttcttcttg gaggccttgg cttcggcctt gttcaccttc tcgtcggcct
     2821 tggcgtcggt cttggcttcg gagttggcgg ccttcgcatc ggccttctcg gccttggatt
     2881 cggccttctg gacgtccttg ctcatggcgg gcgcgctggc ctgggccatc gccatcgaag
     2941 cgaacaggga ggcgatcagg gcggcgagga gcttgctcat tgtcatttcc ttcatgggtt
     3001 tgtgtggagc accgaccgac catcgatcga gtccatgtgc ctgcaacgcg ggacgccggg
     3061 tgcttcgttg acaggctgcg gacttccaca tgcggaagtt ttttcctgtc gcgcgagcga
     3121 aatcggcggc tccgatggtg cgttcgtcgc aagcccaata ctcattggca aacgtcgcgc
     3181 ccacgcacgc tcagggtgct cccgcgtgat gcttcgctcg tgacgattcg acaccgtttc
     3241 gacgcgtggc gcgcgtcgtg cgagcgctaa ccgcgcgacc tgtcgtcgat cagcgacaag
     3301 tcttggaggc taaccttgac atgagtggga caagcgcggg cggacgaaat ccggcttgct
     3361 gctcgacggt ggaaggcgat gcggtcggcg tgctcagggc aacggtcgag cactgcgccc
     3421 gaggcgcgac atgctcaccg cggcgccggc cagcgcgatg cccacgccga tcagcaggca
     3481 caggtgctcg ccttccttgg ccacggtgtg gagcacgagc gcggtgaccg acgcgcccac
     3541 cgtcatgccc gtcagccgcg ccgtcgcgag catgccgccc gcggcgcccg cgcggtcgcg
     3601 cggggcactg cccaacatcg ttcggttgtt gggcgcctgg aagaagccga agcccagtcc
     3661 gcacagcgcc atgcgccaca ggatggacag cgtcgacgcg tcggacggca ggcgcgccat
     3721 cagccacagc ccgcacgcca gcaccagcag ccccgcggtg cccaggatgg ccgcgggcac
     3781 gcggtcggcc aggcgcccgg cgagcggcgc gacgaggccc acgccgacgg gccagggcgt
     3841 catgagcagg ccggtctgca cctggtcgcg gtgcagcgcc gcctggaacc agaacggcag
     3901 cgcgatgaag gccagcgtgt aggccgcgaa cgagcagatc gaggtcagcg tcgacagcgc
     3961 gaacacggga atgcgcagca ggtcgatcgg gatcagcggg cgcgcgtcgt ggcgctcgcg
     4021 caggaacaac acgatgcccg cgacgacgcc ggcgccgacc tcgatcgcgc cgagcgggcc
     4081 cggggcgccg tgcgtgagcg tgtcggcgcc gatgaagaac agcccgaagg ccagcgcgct
     4141 caggccggcg ctgagccagt ccagggcctt gtccgacagc gtcgaccacg gc
//
LOCUS       DATCRO010000666         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1099088, whole genome shotgun sequence.
ACCESSION   DATCRO010000666 DATCRO010000000
VERSION     DATCRO010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099088"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VIN75_18180"
     CDS             <1..751
                     /locus_tag="VIN75_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976148.1"
                     /translation="LTRTSNIYIRAASGYRPGGPNAVLNDPQTGLPTAPTTFQHDSLW
                     SYEAGYKGDLLDNTLSLETAVYDIRWDNIQQAYSVNGNGVLVNAGKAEIQGFEFGATW
                     RPDANWLLEAHVSTIDGRLKEDAPGLGSSGARLPDSPSLAASLGVKSKFDLGGHAGWF
                     GLSQRYAGERNAGFDGSGTAPNYRMPSYWLTDVQGGVDFGKISLGLYARNVFDAHAQL
                     GTSTSEMALGGPAQVELARPRTLGMTLTASF"
     gene            760..1890
                     /locus_tag="VIN75_18185"
     CDS             760..1890
                     /locus_tag="VIN75_18185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8976149.1"
                     /translation="MSVDGQTFDFIVIGGGMAGASAAAHLSRRGAVALLERESQPGYH
                     STGRSAALFSTIYGNAPVRALTRASQAFLFESPSWFTPVPLVSPRPTLFFATQEQSAM
                     LASFREDADVAAHTQVLDAAQAGALVPAFKPGWLGGAVLEPGSADIDVAALHQGYLRQ
                     AKARQAAVLLDCAIAGLHFAGARWMVRTDQGTVSAPVVVNAAGAWGDEVARLAGARPV
                     GLRPLRRTAMLVDPPRQLACAGWPAAIAIDESFYFKPDAGRLLLSPADEEPSPPCDAQ
                     PEDLEVAIAVDRFETATGEHVAKVHHRWAGLRVFSADRTPVVGFDPVAEGFFWLVGQG
                     GYGIQSAEGLGRLAAALACDEGVPADLAAEGVTAEALARARF"
     gene            1926..>3598
                     /locus_tag="VIN75_18190"
     CDS             1926..>3598
                     /locus_tag="VIN75_18190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8976150.1"
                     /translation="MRSLAPLAAVTALALALASARLCAAPVAASAAAAAPAAPAAASA
                     PIAPASPAHELTAQDAQAWLDGLMPTALRTARTPGAVVVIVKDGQVLLEKGYGYSDEK
                     TRAPVDPRRTLFRPGSTSKLFTWTAVMQLVEQGKLDLDTDVNKYLDFQVPPLDGQPIT
                     LRQVMTHRAGFEERIKDLVAFDIPETPLGEVLSGNMPARIYKAGTTSAYSNYATGLAG
                     HIVERVSGLSFNDYIEKNVFAPLGMAHSTFRQPLPDALKADMATGYEESGKPGKGFEV
                     VSIPPAGSLSSTGDDMARFMIAHLADGQYGNARILKPETAHMMHTTITRPFPDLNGMA
                     LGFYQDDINGHPVIAHGGDLNYFHSDLWLFLDDHVGVFISVNALGKDGLGEGIRDDVF
                     HEFADRYFPRPEPVQALDEATAKAHAQAIAGRYQTTRRGETTFLSLINMIQPTTITAN
                     PDGSITTKLLLHPQTFVETKPWLWHEVGGHDRLEARVEDGKVATWSTDYLAFAFAYQP
                     LHGLAGAGLELPLFVLSLVLMLVTVLTWPVGAAARKWYGAAPLPAAVRT"
BASE COUNT          519 a         1322 c         1257 g          500 t
ORIGIN      
        1 gctcaccagg accagcaaca tctacatccg cgccgccagc ggctaccgcc ccggcggccc
       61 gaacgcggtg ctgaacgacc cgcagacggg cctgccgacc gcgcccacca cgttccagca
      121 cgactcgctc tggagctacg aggccggcta caagggcgac ctgctggaca acacgctgtc
      181 gctcgagacg gccgtctacg acatccgctg ggacaacatc cagcaggcct actcggtcaa
      241 cggcaacggc gtgctggtca acgcgggcaa ggcggaaatc cagggcttcg agtttggcgc
      301 gacctggcgt ccggacgcga actggctgct ggaggcccat gtgtcgacga tcgacggccg
      361 cctcaaggag gatgcgccgg gcctgggttc gtcgggtgcg cggctgccgg acagcccgtc
      421 gctggcggcc tcgctgggtg tgaagtccaa gttcgacctg ggcggccacg ccggctggtt
      481 cggcctgtcg caacgctacg cgggcgagcg caacgccggc ttcgacggca gcggcacggc
      541 gccgaactac cgcatgccgt cgtactggct caccgacgtg cagggcggcg tcgacttcgg
      601 caagatctcg ctcgggctgt acgcgcgcaa cgtgttcgat gcgcatgcgc agctgggcac
      661 ctcgacttcc gagatggcac tgggcggtcc ggcgcaggtc gagctggcgc ggccgcgcac
      721 gctgggcatg acgttgacgg ccagcttctg aggatgcgca tgagcgttga cggccagacc
      781 ttcgacttca tcgtgatcgg cggcgggatg gcgggcgcct cggcggccgc gcacctgtcg
      841 cggcgcggcg cggtcgccct cctggaacgc gaaagccagc cgggatatca ctccacgggc
      901 cgctcggcgg cgctgttctc gacgatctac ggcaacgccc cggtgcgcgc gctgacgcgg
      961 gcgagccagg ccttcctgtt cgagtcgccg tcgtggttca cgccagtgcc gctggtgagc
     1021 ccgcgcccga cgctgttctt cgccacgcag gaacagtcgg cgatgctcgc gagcttccgc
     1081 gaggacgccg atgtggccgc ccacacgcag gtgctcgacg ccgcgcaggc cggcgcactg
     1141 gtgcccgcgt tcaagccggg ctggctcggc ggcgcggtgc tggagccggg ctcggccgac
     1201 atcgacgtcg ccgcgctgca ccagggctac ctgcgccagg cgaaggcgcg ccaggccgcg
     1261 gtgctgctcg attgcgcgat cgcgggcctg catttcgccg gcgcgcgctg gatggtgcgc
     1321 accgaccagg gcacggtctc ggcgccggtc gtcgtcaacg ccgcaggtgc ctggggcgac
     1381 gaggtggcgc ggctggccgg cgcgcggccc gtgggcctgc ggccgctgcg gcgcacggcg
     1441 atgctggtcg atccgccgcg ccagctcgcc tgcgccggct ggcccgcggc catcgcgatc
     1501 gacgagagct tctatttcaa gcccgacgcc ggccggctgc tgctgtcgcc cgccgacgag
     1561 gagccgagtc cgccgtgcga tgcccagccc gaggacctcg aggtcgccat cgccgtcgac
     1621 cgcttcgaga cggccaccgg cgagcacgtc gcgaaggtgc accaccgctg ggccgggctg
     1681 cgcgtgttca gcgccgaccg cacgccggtg gtcggcttcg accccgtcgc cgagggcttc
     1741 ttctggctcg tgggccaggg cggctacggc atccagagcg cggagggcct cgggcgcctg
     1801 gccgccgcgc tcgcctgcga cgagggcgtg cccgccgacc tcgccgccga gggcgtgacc
     1861 gccgaggcac tcgcccgcgc ccgcttctga caagtcatcc gaagtccaac ccaccaggaa
     1921 tgccgatgag atcactcgct cccctcgccg ccgtcaccgc gctggcgctc gcgcttgcgt
     1981 ccgcgcgcct gtgcgccgcg ccggtcgcgg catcggccgc cgccgccgcg ccggcggcgc
     2041 ctgccgcggc cagtgcgccg atcgcgcccg cgtcgccggc ccacgagctc accgcgcagg
     2101 acgcgcaggc ctggctcgac gggctgatgc ccaccgcgct gcgcaccgcg cgcacgccgg
     2161 gcgccgtcgt cgtcatcgtc aaggacggcc aggtgctgct cgagaagggc tacggctatt
     2221 ccgacgagaa gacgcgcgcg ccggtcgacc ccaggcgcac gctgttccgc ccgggctcga
     2281 cgtccaagct gttcacgtgg acggccgtga tgcagctggt ggagcagggc aagctcgacc
     2341 tggacaccga cgtcaacaag tacctcgact tccaggtgcc gccgctggac ggccagccga
     2401 tcacgctgcg ccaggtcatg acgcaccgcg ccggcttcga ggaacgcatc aaggacctgg
     2461 tggcgttcga catccccgag acgccgctgg gcgaggtgct gagcggcaac atgccggcgc
     2521 gcatctacaa ggcgggcacg acctcggcct actcgaacta cgcgaccggc ctggccggcc
     2581 acatcgtcga gcgcgtgtcg ggcctgtctt tcaacgacta catcgagaag aacgtgttcg
     2641 cgccgctggg catggcgcac tcgaccttcc gccagccgct gcccgacgcg ctcaaggccg
     2701 acatggccac cggctacgag gaatcgggca agccgggcaa gggcttcgag gtggtcagca
     2761 tcccgcccgc gggctcgctg tcgtccaccg gcgacgacat ggcgcgcttc atgatcgccc
     2821 acctggccga cggccagtac ggcaacgcgc gcatcctcaa gccggagacg gcgcacatga
     2881 tgcacacgac gatcacgcgc ccgttccccg acctgaacgg catggcgctg ggcttctacc
     2941 aggacgacat caacggccat ccggtgatcg cgcacggcgg cgacctgaac tatttccaca
     3001 gcgacctgtg gctgttcctg gacgaccacg tgggcgtgtt catctccgtc aacgcgctcg
     3061 gcaaggacgg cctgggcgag ggcatccgcg acgacgtgtt ccacgagttc gcggaccgct
     3121 acttcccgcg cccggagccg gtgcaggcgc tggacgaggc gaccgccaag gcgcatgcgc
     3181 aggccatcgc gggccgctac cagaccacgc ggcgcggcga gacgacgttc ctgtcgctga
     3241 tcaacatgat ccagccgacc acgatcacgg ccaatcccga cggctcgatc acgaccaagc
     3301 tgctgctgca cccccagacc ttcgtcgaga ccaagccgtg gctgtggcac gaggtcggcg
     3361 gccacgaccg gctcgaggct cgcgtggagg acggcaaggt cgcgacctgg agcaccgact
     3421 acctcgcctt cgcgttcgcc taccagccgc tgcacggcct ggccggcgcc ggcctcgagc
     3481 tgccgctgtt cgtcctgtcg ctcgtgctga tgctcgtgac ggtgctgacg tggccggtcg
     3541 gcgcggccgc gcgcaagtgg tacggcgcgg cgccgctgcc cgcggcggtg cgcacgag
//
LOCUS       DATCRO010000667         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_424224, whole genome shotgun sequence.
ACCESSION   DATCRO010000667 DATCRO010000000
VERSION     DATCRO010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424224"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIN75_18195"
     CDS             complement(<1..432)
                     /locus_tag="VIN75_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8976151.1"
                     /translation="MSIRSFAADLLPAKLHGLARAPAAAPQQAEVTTFAELSEKLGYL
                     SKSDLKKVREAYKFADEAHLGQLRASGIPYITHPLAVTGLAADWKLDATALMAALMHD
                     TIEDCGVTKPELIERFGAPTADLVDGLTKLDKLQFSTREDGQ"
     gene            780..2555
                     /locus_tag="VIN75_18200"
     CDS             780..2555
                     /locus_tag="VIN75_18200"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HEY8976152.1"
                     /translation="MIPRVAGLAVLLAFSLGSAGYVQAQTASDYTQGVTVSGTTATIN
                     FNPTSSTTTWTDVHYTVNGGAQQNYRMARNATTGAETQQVMQAVSAGNVIAYSFTYNK
                     GTPAYDTPTYSYTVGSSTGGGTSVNPAAGQACFFADINYGGDSFCASASSSWVGSAWN
                     DRISSVKVASGVTVVLYNDVNYGGSTLTVSANNANLVNNGFNDVASSFKLTTGSTGGT
                     WNKQTTFNIVNGTNNAYDNAHVFWAIIGKDWTSGKFVWVNASGQLIPMSTADNGALTK
                     NGVGYTNYFHSIAQAGSVTIPPINSARLLLSVGTPMYIRVNQDVNGNIGYAGANIDNP
                     TDPNIDVTFDFAEMAILPDTGFYGNTTRVDQFGFPVTLRLQGLDGYDQTVGETASRAS
                     LFSAWASSVPAQFAGLGQAPYSPYRIVAPAHGSFGASGANAHYLDSYIQGLWSKYTTQ
                     TLTFTDQQGTFTGHVVNGQFQFTDGQGTYIIQRAPTTQEALLGNGVLNDATGQTPGTA
                     GYDKQLQIQAQMCAAINRHIVEDPAHWYTPSYFYANPSNSYSKFWHDHSINALSYGFA
                     YDDVGGFSSSLHTEKPTIATVTVGW"
     gene            complement(2902..3105)
                     /gene="rpoZ"
                     /locus_tag="VIN75_18205"
     CDS             complement(2902..3105)
                     /gene="rpoZ"
                     /locus_tag="VIN75_18205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY8976153.1"
                     /translation="MARITVEDCLEKIPNRFQLVLAATYRARMLSQGHTPKIESKNKH
                     GVTALREIAAGEIGKEMLRKVPT"
     gene            complement(3183..3809)
                     /gene="gmk"
                     /locus_tag="VIN75_18210"
     CDS             complement(3183..3809)
                     /gene="gmk"
                     /locus_tag="VIN75_18210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8976154.1"
                     /translation="MELPGKLFVVAAPSGAGKSTLVKALLAHEPHLAVSVSHTTRAPR
                     GQEQDGREYHFTDVGTFRRMVGDGQFFEHAHVHGNWYGTSRAGVESLLAAGKDVVLEI
                     DYQGALQIRALFPAALLVFILPPSWDELAQRLRGRGEDAPEVIAQRLANARNEVAQAP
                     KFDFVIINGSLDNALVELRAVVREQPGALRFEAQRANHPDVFAALGLA"
     gene            complement(3906..4607)
                     /locus_tag="VIN75_18215"
     CDS             complement(3906..4607)
                     /locus_tag="VIN75_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HEY8976155.1"
                     /translation="MLRHSPLRRLGALALVCAGLATAPAVFAQPESPVADDARPATQP
                     LSDLAGSLQVGDIVFTRIGAYPFRKVAEATGTWTNHVGIVLDVSGKEPTIGESRFPLS
                     GTTTLSRFVARSAGGRVAVMRLPAPLTPAQQSALLAAATQRAHVFYDTGFDAHSSRQF
                     CSRYVREVLQQAAGVQVGEVQTFQQLLASAPHADVAFWRVWYFGSIPWQRETVTPASL
                     LHTPGLKPVFDGVAA"
BASE COUNT          805 a         1594 c         1648 g          728 t
ORIGIN      
        1 ctggccgtcc tcgcgcgtcg agaactgcag cttgtccagc ttggtcaggc cgtcgacgag
       61 gtcggcggtg ggcgcgccga agcgctcgat cagctcgggc ttggtgacgc cgcagtcctc
      121 gatggtgtcg tgcatcagcg cggccatcag cgccgtggca tcgagcttcc agtcggccgc
      181 cagccccgtg accgcgagcg ggtgcgtgat gtacgggatg ccgctggcgc gcagctggcc
      241 caggtgcgcc tcgtcggcga acttgtaggc ctcgcgcacc ttcttcaggt cggacttgct
      301 gaggtagccc agtttctcgg acagctcggc gaaggtggtg acctcggcct gctggggcgc
      361 ggcggccggg gcgcgcgcca ggccgtgcag cttggcaggc agcaggtcgg cggcgaacga
      421 acgaatgctc atgaccaaga atttagcttg ttatcaggcc ggcgccgcca cgctcgggaa
      481 atcgccgtgc ccggccgacc gaccagttcc agaccgatcg agcgcccggt accgcgggtt
      541 ttcgccatcc cctgtttcgg tgattctttg aaaccgcttt caaaaaatta cccgaatggc
      601 ggtcacaaag gccttttcag gagtcaaatg cgctccgccc cggctgctcc aggcaggcgg
      661 cgggggcaag cccaagtgcc ggacgcgatg cccgaacgtc gccccggccc tgcctggaca
      721 cagactggcc tgagaagcga cgcgcgcgaa cgcgtcaacc aaaacgacag gagacagcga
      781 tgattccacg agttgcaggg cttgccgtcc tgctggcatt ttccctcggc agcgcaggct
      841 acgtgcaggc ccagacggcc tccgactaca cgcagggcgt gacggtcagc ggcacgacgg
      901 ccacgatcaa tttcaacccg acctcgagca ccacgacctg gacggacgtc cactacacgg
      961 tcaatggcgg cgcgcagcag aactaccgga tggcgcgcaa cgcgaccacc ggcgcggaga
     1021 cccagcaggt catgcaagcg gtttcagccg gcaacgtgat cgcctacagc ttcacgtaca
     1081 acaagggcac gccggcgtac gacacgccga cctactcgta caccgtgggc tcgtccaccg
     1141 gcggcggcac gtcggtgaac ccggcggccg gccaggcctg cttcttcgcc gacatcaact
     1201 acggcggcga ctcgttctgc gccagcgcca gcagcagctg ggtcggcagc gcgtggaacg
     1261 accgcatctc gtcggtcaag gtggcgtcgg gcgtgacggt cgtcctgtac aacgacgtca
     1321 actacggcgg ctccacgctg accgtctcgg ccaacaacgc caacctggtc aacaacgggt
     1381 tcaacgacgt cgcctcgtcg ttcaagctga ccaccggttc gaccggcggc acctggaaca
     1441 agcagacgac gttcaacatc gtcaacggca ccaacaacgc ctacgacaac gcgcacgtct
     1501 tctgggccat catcggcaag gactggacga gcggcaagtt cgtgtgggtc aacgcctccg
     1561 gccagctgat cccgatgtcc accgcggaca acggcgcgct gaccaagaac ggcgtcggct
     1621 acaccaacta cttccactcg atcgcgcagg ccggctcggt gacgattccg ccaatcaact
     1681 cggcgcggct gctgctgagc gtgggcaccc cgatgtacat ccgcgtcaac caggacgtga
     1741 acggcaacat cggctacgcc ggcgccaaca tcgacaaccc gaccgacccg aacatcgacg
     1801 tcacgttcga cttcgccgag atggccatcc tcccggacac cggcttctac ggcaacacga
     1861 cgcgcgtcga ccagttcggc ttcccggtca cgctgcgcct gcagggcctg gacggctatg
     1921 accagacggt gggcgagacg gcctcgcgcg cctcgctgtt ctcggcctgg gcctcgtcgg
     1981 tgccggcgca gttcgcgggc ctcgggcagg cgccgtactc gccgtatcgc atcgtcgccc
     2041 cggcgcacgg cagcttcggc gcctcgggcg cgaacgcgca ctacctggac agctacatcc
     2101 agggcctgtg gagcaagtac acgacgcaga cgctgacgtt caccgatcag cagggtacgt
     2161 tcacgggcca cgtcgtcaac ggccagttcc agttcacgga cgggcagggc acttacatca
     2221 tccagcgcgc gccgacgacg caggaagcgc tgctgggcaa cggcgtcctc aacgacgcca
     2281 ccggccagac gccgggcacc gcgggctacg acaagcaact gcagatccag gcccagatgt
     2341 gcgcggccat caaccgccac atcgtcgagg atccggcgca ctggtacacg ccgagctact
     2401 tctacgccaa cccgtcgaac tcgtactcga agttctggca cgaccacagc atcaatgcgc
     2461 tgtcgtacgg cttcgcgtat gacgacgtcg gcggcttcag ctcgtcgctg cacacggaga
     2521 agccgacgat cgcgaccgtg acggtcggct ggtaagaggc gagaagggct ccgcgcctgc
     2581 gggcgcggat catggggagg ccggaagcgg cgcaggaacg accgcttgcc gcttccggcc
     2641 tgtttttcgc cggcggcggt gggtgtcgcc ggtgttcgaa gcctgtgtcg ctcgcgatgc
     2701 aggctttgtt tttgtccttg tatccgggat ccggtgtcag gtcttgaatc gtgcagtctg
     2761 cgtgtttgcg ggcggctcgt tcgcctgcgg tgccccgccc cacccaaccc gaagcgctca
     2821 agaaagcaaa aagcccgagt cgcatcgaag cgatcgggcc ttccggagac gaaccgcgag
     2881 aacgcggtgt ggcggatcag gtcaggtcgg aaccttgcgc agcatttcct tgccgatctc
     2941 gccggcggcg atctcgcgca gggcggtcac gccgtgcttg ttcttcgatt cgatcttggg
     3001 cgtgtggccc tggctcagca tgcgggcgcg gtacgtggcg gcgagcacga gctggaagcg
     3061 gttcgggatc ttttccaggc agtcttcaac ggtgatgcgg gccatgtggc ttccttggct
     3121 gtgaccggtg cgggaacgcg ccggacgggg aactggacgt tcgatctgcc ggccgacgca
     3181 agtcaggcca ggcccagtgc ggcgaagacg tcggggtggt tggcgcgctg ggcctcgaag
     3241 cgcagggccc cgggctgctc gcgaacgacc gcgcggagtt ccaccagggc gttgtcgaga
     3301 gaaccattga ttataacaaa gtcgaacttc ggggcctggg cgacctcgtt gcgggcgttg
     3361 gccaggcgct gggcgatgac ctcgggggcg tcctcgccgc ggccgcgcag gcgctgggcc
     3421 agctcgtccc agctgggcgg caggatgaac accagcagcg cagccgggaa cagcgcgcgg
     3481 atctgcagcg cgccctggta gtcgatctcg agcacgacgt ccttgccggc cgccagcagc
     3541 gactcgacgc ccgcgcgcga ggtgccgtac cagttgccgt gcacgtgcgc gtgctcgaag
     3601 aactggccgt cgccgaccat gcgccggaag gtgccgacgt cggtgaagtg gtattcgcgc
     3661 ccgtcctgct cctgcccgcg cggcgcgcgc gtggtgtgcg acaccgagac cgccaggtgc
     3721 ggctcgtgcg ccagcagcgc cttcacgagg gtcgatttgc cggcgccgct gggggcggcg
     3781 acgacgaaga gcttgccggg gagttccatg gaaacgagcg cgacgggtgg ggtttcgcca
     3841 gcctcgaagt atcgccgcaa tccccgtgcg ccatggcgcc ggcagcgtga ggaatcctcg
     3901 tcttctcagg ccgcgacgcc gtcgaacacc ggcttcaggc cgggcgtgtg cagcaggctg
     3961 gcgggcgtga ccgtctcgcg ctgccacggg atgctgccga agtaccagac gcgccagaag
     4021 gcgacgtcgg catgcggcgc gctggcgagc agttgctgga acgtctgcac ctcgccgacc
     4081 tggacgcccg cggcctgctg gagcacctcg cgcacgtagc gcgagcagaa ctggcgcgac
     4141 gaatgcgcgt cgaagcccgt gtcgtagaag acgtgcgcgc gctgcgtggc cgccgcgagg
     4201 agcgccgact gctgggcggg cgtcagcggc gcgggcagcc gcatcaccgc cacgcggccg
     4261 ccggccgagc gggcgacgaa gcggctcagc gtcgtggtgc cggacaacgg gaagcggctc
     4321 tcgccgatcg tcggctcctt gccggagacg tccagcacga tgcccacgtg gttggtccag
     4381 gtgcccgtgg cctcggcgac cttgcggaac gggtaggcgc cgatgcgcgt gaagacgatg
     4441 tcgccgacct gcagcgagcc ggccaggtcg ctcagcggtt gcgtggccgg cctcgcgtcg
     4501 tcggcgaccg gggactccgg ttgcgcgaaa acggcgggtg cggtggccag gccggcgcag
     4561 acgagggcga gggcgccgag acggcgcagc ggagagtgac gaagcatggg cgtggccgca
     4621 cgggcgggtc gtttgatgag gggcgcagca tcgcgcatcg ctccgcaggt ggaaaccgag
     4681 ccgttcgagg tggagttccg aacgcccgca ggtatcacat cacgataccc gctgggacag
     4741 gcggagcgtg ccgcaggacc cggcctgtcg gcggg
//
LOCUS       DATCRO010000668         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_63681, whole genome shotgun sequence.
ACCESSION   DATCRO010000668 DATCRO010000000
VERSION     DATCRO010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63681"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VIN75_18220"
     CDS             complement(<1..1091)
                     /locus_tag="VIN75_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY8976156.1"
                     /translation="MSAADEDAVHAPDPASPRHRWHFRVLASTAATLRLWVWAAAAGA
                     AAAAATLAFRWLTLQIEWLGTGQSSDLVEAARAIPAWQRPIVCAVGGVLSGLVLQYGA
                     RWVARSKQGDHYLDYIDAARQGRVDLNDRANLVRTVSAALSIGTGASIGREGPMVQLA
                     AWASAWLARIVPIRPEQRSAIMVCGIAAGIAAAYHAPVAGVVFVLELALGFFARHTVA
                     PVLIASGTASALVYVMVDPKPLYVTSPIAMLPSSILFTLVASVAFGGLGWGFLLLVER
                     SRALFARLQSPAVRLGVGGLLAGALSAFVPEVWGNGYSAVERALAGTEPWQWLALVLV
                     TKVVATSWSSGSGAIGGMFTPSLFVGAAAG"
     gene            complement(1116..1601)
                     /locus_tag="VIN75_18225"
     CDS             complement(1116..1601)
                     /locus_tag="VIN75_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976157.1"
                     /translation="MSNDIDTALEAEVIRRAVALGIRTRVPGRRWRAARLAARLYAGA
                     TAVQRSRIVTTLCRPLGPLALVAVASGAFAGLLDRAGALGGTLQIDELAGFTRDQVFE
                     LARFVEQVSPEALQQVAGSVVENPWGVSALGAAAAVLLATAARRPDAAPRDGPEAAPA
                     A"
     gene            complement(1860..>2837)
                     /locus_tag="VIN75_18230"
     CDS             complement(1860..>2837)
                     /locus_tag="VIN75_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976158.1"
                     /translation="PAETGDAGSTSGFVGNLHDPVYCPNGVVQPNLPAGMLDPCNIAL
                     QELQLSSPHLKPEKSTSFTLGTIFEPNKIYSATLDYYHITIRDQIISVGQLGQIGLDD
                     AASLGTIIYRDSSQNILYDTYPFINANTTTTSGLDLDLVGKYDLHEAGSLKAELQLSY
                     MLQYDLTAQGVRYHLAGTHGPAFVSEDTGTPRTRGAFTLTWGHGPFEVAGTVNYISHF
                     SVTDPSEGIPDCATALSSVFPDGNAPSKFCHVSSFTELNVVARYNWDSHLQLHASVTN
                     LLNRKAPYDLQTFGAPGNGAEQGGAPYNPSYHQDGAVGQMFTIGGTYTF"
BASE COUNT          425 a          986 c         1043 g          383 t
ORIGIN      
        1 cccgccgcgg cgccgacgaa cagcgacggc gtgaacatgc cgccgatcgc gccgctgccc
       61 gaactccacg acgtcgccac gaccttggtc acgagcacca gcgcgagcca ctgccagggc
      121 tcggtgcccg cgagcgcgcg ctccacggcg ctgtagccgt tgccccagac ctcgggcacg
      181 aaggccgaca gcgcccccgc cagcaggccg cccacgccca gccggacggc gggcgattgc
      241 aggcgggcga acagcgcgcg actgcgctcg accagcagca ggaagcccca gccgaggccg
      301 ccgaacgcga cgctggccac cagcgtgaac aggatgctgg agggcagcat cgcgatgggg
      361 ctcgtgacgt acaggggctt gggatcgacc atcacgtaga ccagcgcgct ggccgtgccc
      421 gaggcgatca gcaccggcgc gaccgtgtgg cgcgcgaaga agccgagcgc gagttccagc
      481 acgaacacga cgccggccac cggcgcgtgg tacgccgcgg cgatgccggc cgcgatgccg
      541 cacaccatga tcgcgctgcg ctgctcgggc cggatcggca cgatgcgcgc gagccacgcg
      601 gacgcccacg cggccagctg caccatcggg ccctcgcggc cgatcgacgc gccggtgccg
      661 atcgacagcg cggccgacac cgtgcgcacg aggttggcgc gatcgttgag gtcgacccgg
      721 ccctgccgtg cggcatcgat gtagtcgagg tagtggtcgc cctgcttgct gcgcgcgacc
      781 cagcgcgcgc cgtactgcag caccaggccc gacagcacgc cgccgaccgc gcacacgatg
      841 ggccgctgcc acgcggggat ggcacgcgcg gcctcgacga ggtcgctgct ctggccggtg
      901 cccagccact cgatctgcag cgtcagccag cggaacgcca gcgtcgcggc ggcggccgcg
      961 gcgccggccg ccgcagccca gacccacagc cgcagcgtgg ccgcggtcga ggccagcacg
     1021 cggaagtgcc agcggtggcg cggggaggcc gggtcggggg cgtggacggc gtcttcgtcg
     1081 gcggcggaca tggccccatt gtcggggcaa cgcggtcagg ctgccggtgc cgcctccggg
     1141 ccgtcccgcg gcgccgcgtc cgggcgacgc gccgccgtcg ccagcagcac ggccgcggcc
     1201 gcgcccagcg cggatacgcc ccacgggttc tccacgacgc tgccggccac ctgctgcaag
     1261 gcctcggggc tcacctgctc gacgaagcgc gccagctcga acacctggtc gcgcgtgaag
     1321 ccggccagct cgtcgatctg caacgtgccg ccgagcgcgc ccgcgcgatc gagcaggccc
     1381 gcgaacgcgc ccgaggcgac cgcgaccagc gcgagcggcc ccagcggccg gcacagcgtc
     1441 gtgacgatgc gcgagcgctg caccgccgtc gcgccggcgt acagccgcgc ggccaggcgc
     1501 gccgcgcgcc agcgcctgcc ggggacgcgg gtccggatgc ccagcgcgac cgcgcgccgg
     1561 atgacttcgg cctccagggc cgtgtcgatg tcattgctca tcgttcttgc tccgccatgc
     1621 tttccgaagc gatggagtgg ctcgaaacag cgcggttccg cggcgatcgc gtatggcaat
     1681 gtaaaaacgc agccgccttc aggatcatga aatcggcgcg tcatctcgcg ttcacgaagc
     1741 ggtcacgccg tgcccgcatg caggcggatg gcgatccggg cggtgaaggc tcggcgcctg
     1801 aatggcgcca gtcaggactg cagcgcagcc gcccggacat gcgccgcgcg tcgcatgcgt
     1861 cagaacgtgt aggtgccccc gatcgtgaac atctgcccga cggcgccgtc ctggtgatag
     1921 gacgggttgt agggcgcgcc gccctgttcg gcgccgttgc ccggcgcgcc gaaggtctgc
     1981 aggtcgtacg gcgccttccg gttcaggagg ttggtgacgc tggcatgcaa ctgcaggtgg
     2041 ctgtcccagt tgtagcgggc cacgacattg agctccgtga acgaggagac gtggcagaac
     2101 ttcgacggcg cgttgccgtc ggggaacacg ctggacagcg cggtggcgca atcagggatc
     2161 ccctcgctgg ggtcggtgac gctgaagtgc gagatgtagt tgaccgtgcc cgcgacctcg
     2221 aacggcccgt ggccccacgt cagcgtgaac gcgccgcgcg tgcgtggcgt cccggtgtct
     2281 tccgagacga acgccgggcc gtgcgtgccg gcgaggtggt agcgaacgcc ctgcgcggtg
     2341 aggtcgtact gcagcatgta gctcagctgc agttcggcct tgaggctgcc ggcctcgtgc
     2401 aggtcgtact tgccgaccag gtcgaggtcg aggccggagg tcgtcgtcgt gttcgcgttg
     2461 atgaacgggt aggtgtcgta gaggatgttc tgcgaggagt cgcggtagat gatcgtgccc
     2521 aggctcgcgg cgtcgtccag cccgatctgg cccagctggc ccaccgagat gatctggtcg
     2581 cggatcgtga tgtggtagta gtccagcgtc gccgaataga tcttgttcgg ctcgaagatc
     2641 gtgcccagcg tgaacgacgt cgacttctcg ggcttgaggt gcggcgacga cagctgcagc
     2701 tcctgcagcg cgatgttgca cgggtcgagc atgcccgcgg gcaggttggg ctgcacgaca
     2761 ccgttggggc agtacacggg atcgtgcagg ttgccgacga agccggaggt ggagccggca
     2821 tcgccggtct cggcggg
//
LOCUS       DATCRO010000669         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_817423, whole genome shotgun sequence.
ACCESSION   DATCRO010000669 DATCRO010000000
VERSION     DATCRO010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817423"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VIN75_18235"
     CDS             complement(<1..1619)
                     /locus_tag="VIN75_18235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY8976159.1"
                     /translation="MPQAIPAHYRAIAGSHRQASASARRLGPADAGEKVQVTISLRRR
                     TDGPPAPDFDTVATVPLSQRQPMSQKDFAARYGAHPDDIRKVEDFVQTAGMTVVESNA
                     ARRTVVASGTVAQMNQAFAVDLGKYSAPAPARGHRAQPVIETYRGREGDVHVPAALDG
                     IVLGVFGLDNRTVLKRNSADPANTHTLTIPTLKQLYNFPTNSAAGQTIGIVSLSGYAI
                     HDIQLLFNGLGAGYTMPTVHDVAVHGGNSGSDPYGETTQDIGIAAAAANGAAIAVYIT
                     NGTQAGWVDLVHRVAHPNAGDPSCSVLSSSWYISNGDDAATLAAEGVTTSFINAVTAA
                     FQDAAIQGITVCIACGDTGSNSKVNDGKAHVQYPGSDPWVLSVGGTTVGNVSGSTFEE
                     YVWNDPSPSDPSHWGTTGGGVSDFFNLPSYQAYARVPASVNAGHRVGRGLPDVSANAS
                     YNAGYAGLYLNGNPMIGNGTSASAPLWAGFVAKLNAALGTRLGFINPILYAKGTLGFR
                     DVVPGGGPANNANGGTPGYPAGDGWDACTGWG"
     gene            complement(1883..>2908)
                     /locus_tag="VIN75_18240"
     CDS             complement(1883..>2908)
                     /locus_tag="VIN75_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY8976160.1"
                     /translation="ALAFVDRVHAQPGGGYAWVLDWHDGQRRVIDATNHCYGLAFVLL
                     AHAHALMAGQADAAEGLERCWQLMEQRFWRPRDSLYADEATPDWTVGPYRGQNANMHA
                     CEAMQAAWRATKDRKYLDRAVVLAEAVTGRLGAASADLPGVIPECASLVWEHFREDWA
                     IDPDYNRGDRTNIFRPWGFQTGHQTEWTKLLLQLDRLCAEAGLAPVPERLARARGFFD
                     AAMHFGWDDAHGGLVYGFAPDGTLYDGDKYHWVQAESLAAAAWLAVALQQSGAPAADV
                     AHYWNWYDRLWAYAWAHFVDHEYGAWYRILAADNAKVTDEKSPAGKVDYHDMGACYDV
                     LAALREI"
BASE COUNT          378 a         1006 c         1067 g          457 t
ORIGIN      
        1 ccccagccgg tgcaggcgtc ccagccgtcc cccgccggat agccgggcgt gccgccgttg
       61 gcgttgttgg ccgggccgcc gccgggcacg acgtcgcgga agccgagcgt gcccttggcg
      121 tacaggatcg ggttgatgaa gcccaggcgc gtcccgagcg cggcgttgag cttggccacg
      181 aagccggccc acagcggcgc cgaggcgctg gtgccattgc cgatcatcgg gttgccgttc
      241 aggtacagcc cggcgtaccc ggcgttgtag ctcgcgttgg ccgacacgtc gggcagcccg
      301 cggccgacgc ggtgtcccgc gttcaccgag gccggcacgc gggcgtaggc ctggtaggac
      361 ggcaggttga agaagtcgct gacgccgccg ccggtggtgc cccagtgcga cgggtcggac
      421 gggctcgggt cgttccagac gtattcctcg aaggtcgagc ccgacacgtt gcccaccgtc
      481 gtgccgccca ccgacagcac ccacgggtcg ctgcccgggt actgcacgtg cgccttgccg
      541 tcgttgacct tggaattgga gccggtgtca ccgcaggcga tgcacacggt gatgccctgg
      601 atcgcggcgt cctggaacgc ggcggtcacg gcattgatga acgaggtggt gacgccctcg
      661 gccgccagcg tcgccgcatc gtcgccgttg gagatgtacc agctgctcga aagcacggag
      721 cacgacgggt cgcccgcgtt cggatgcgcc acgcggtgca cgaggtcgac ccagccggcc
      781 tgcgtgccgt tggtgatgta gaccgcgatg gccgcgccgt tggccgcggc ggcggcgatg
      841 ccgatgtcct gcgtcgtctc gccgtacggg tcgctgcccg agttgccgcc gtgcacggcc
      901 acgtcatgca ccgtgggcat cgtgtagccc gcgcccaggc cgttgaacag cagctggatg
      961 tcgtggatcg cgtagccgct cagcgagacg atgccgatcg tctgcccggc cgcggagttg
     1021 gtggggaagt tgtagagctg cttgagcgtg gggatcgtca gcgtgtgcgt gttcgcgggg
     1081 tccgcgctgt tgcgcttgag cacggtgcgg ttgtccaggc cgaacacgcc cagcacgatg
     1141 ccgtcgaggg cggcaggcac gtgcacatcg ccctcgcggc cgcgataggt ctcgatgacg
     1201 ggttgcgcgc gatggccgcg cgccggcgcc ggcgcgctgt acttgcccag gtcgaccgcg
     1261 aacgcctggt tcatctgcgc cacggtgccg gaggcgacga cggtgcggcg cgcggcgttg
     1321 ctctcgacga cggtcatgcc ggccgtctgg acgaagtcct ccaccttccg gatgtcgtcc
     1381 ggatgggcgc cgtacctggc cgcgaagtcc ttctgcgaca tcggctggcg ctgcgacagc
     1441 ggcacggtcg cgaccgtgtc gaagtccggc gcgggcggcc cgtcagtgcg ccggcgcagc
     1501 gagatggtga cctgcacctt ctcgccggcg tcggcgggtc cgaggcgcct ggccgaggcg
     1561 ctcgcctggc gatggcttcc cgcgatggca cgatagtggg cggggatggc ttggggcatc
     1621 tgcggtctcc tggaatggct ccgcccgatg cgggacccgg ccccgggtcc gcaggcgtcg
     1681 cgttgggatg ccgcggatct tgcgcgtcga catcgcgacg cacccatccc ctgcagttgg
     1741 gggacaggcc gcgcatcccc tgtttggagc cctcgtcgcg tcgcgatgcg ccgctgcacg
     1801 ctatggttag cgcgcggccg agagctttcg atgcggactt cgacaggccg cgttcagttc
     1861 caggacagac cgcatggccg cgtcagatct cgcgcagcgc ggccagcacg tcgtagcacg
     1921 cgcccatgtc gtggtagtcg accttgccgg cggggctctt ctcgtcggtc accttcgcgt
     1981 tgtcggcggc caggatgcgg taccaggcac cgtactcgtg gtcgacgaaa tgggcccagg
     2041 cgtaggccca caggcggtcg taccagttcc agtagtgcgc gacgtcggcg gccggcgcgc
     2101 ccgactgctg cagcgccacg gccagccacg ccgcggcggc caggctctcg gcctgcaccc
     2161 agtggtactt gtcgccgtcg tagagcgtgc cgtcgggcgc gaagccgtag acgaggccgc
     2221 catgcgcgtc gtcccagccg aagtgcatcg cggcgtcgaa gaagcctcgc gcgcgcgcca
     2281 ggcgctcggg cacgggcgcg aggccggcct cggcgcacag ccggtccagc tgcagcagca
     2341 gcttcgtcca ttcggtctgg tggccggtct ggaagcccca ggggcggaag atgttggtgc
     2401 ggtcgccgcg gttgtagtcg gggtcgatgg cccagtcctc gcgaaagtgc tcccacacca
     2461 gcgacgcgca ctcggggatc acgcccggca ggtcggccga cgccgcgccc agccggccgg
     2521 tgacggcctc ggccagcacg acggcgcggt ccaggtactt gcgatccttc gtcgcgcgcc
     2581 aggccgcctg catcgcctcg cacgcgtgca tgttggcgtt ctggccgcgg tacgggccga
     2641 ccgtccagtc gggcgtcgcc tcgtccgcgt acagcgaatc ccggggacgc cagaagcgtt
     2701 gttccatcag ctgccagcag cgctccaggc cctcggccgc gtcggcctgg ccggccatca
     2761 gcgcgtgcgc gtgcgccagc aggacgaagg ccaggccgta gcagtggttc gtggcgtcga
     2821 tgacgcgacg ctgcccgtca tgccagtcca gcacccacgc gtagccgccc ccgggttgcg
     2881 catgcacccg gtcgacgaag gccagcgc
//
LOCUS       DATCRO010000670         7930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_862749, whole genome shotgun sequence.
ACCESSION   DATCRO010000670 DATCRO010000000
VERSION     DATCRO010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862749"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="VIN75_18245"
     CDS             complement(<1..1654)
                     /locus_tag="VIN75_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976161.1"
                     /translation="MALSGFDERSVFRSIFSAHPDAMLLVDAAGNIVLSNPAAESLLG
                     YSADQFARMSVDELVPDAIRPRHAAYREAYGKSPRPRPMGTQMDLVARRGDGSEVVVE
                     IALSPLQDHGLPFVVASIRDVGSYPRVRQALQRARYSDRLAQLGRLAVDERDMHVLVE
                     QACRLAVEALDVHSAVVCELAPGGREFVIAGGHALLAEQQVGLRLASEPTTLPGYLVT
                     HPQTILLPDYRLEQRFALPAHLLEAGFVSGVAAPLVDRGRVFGTLTIHARASTRFGED
                     ETRFVESLCSLLATCLQRGRTEEALSHSQRLETVGQLTGGIAHDFNNLLTVIKGNLQI
                     IEDSPGPTPVGDVRPMLASAGRATHRAAELTAKLLAFSRRQMLQPESIDLRGMLDSLA
                     DMLRRTVDQRVRIEVDCPPGCPAVYADPVQLESAMLNVAINARDAMPEGGRIRIGAHN
                     YPAGGEVRRDMVRLSIRDTGQGIEPELLEKVFEPFFTTKGVGTGTGLGLSQVYGFVRS
                     SGGEVKIESVVGRGTTVFLMFPRSSQSLPRDAMESGRERRAEH"
     gene            1995..2306
                     /locus_tag="VIN75_18250"
     CDS             1995..2306
                     /locus_tag="VIN75_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY8976162.1"
                     /translation="MTTRRAFIQILPLAGAAALCANAALAAGVASVDPKDPQAVALGY
                     VPDAAKADKAKYPKYAAGQACGSCQLYQGKPADAAAPCALFGGKQVAGKAWCSAYVKK
                     A"
     gene            2318..2908
                     /locus_tag="VIN75_18255"
     CDS             2318..2908
                     /locus_tag="VIN75_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8976163.1"
                     /translation="MRPALRVLRDEHDALSALLHTLVLMLAQARANHRPPDFGALRAM
                     LFYVDEFPEKRHHRKESELLFPRLRARTPLARDVLDRLDDDHERGHARIRDVEHALTE
                     YEMLGPGRGADFERALQRYVDFYRAHMALEEDEIFPLAERHLTDEDWQHLEAELAGEP
                     DPLTGRSADEEYRALFDRIVGLVPAPFGLAAPSSPP"
     gene            complement(2921..4189)
                     /locus_tag="VIN75_18260"
     CDS             complement(2921..4189)
                     /locus_tag="VIN75_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976164.1"
                     /translation="MRPSFPFETIAPAPPAALLRRFDRGASRHPALPPFADFSPSFGA
                     GELREALERRGQDPGRARRLPLHLAVRLPCEGCIDAKRSASALAGEAAALSDAVGAPQ
                     QVTRVTVQGLGGASAAGGRLGDVLDRICAQFRMEAAEVVAEARWPGASALRSWRAAGV
                     SSLVIEAVRSAEVESARELGFKMVTVRVACGRSGQDGDLLAQELGAMLHAGATRVHLH
                     PYASTHESACDRCPTPPGSIALFEDRGVLRARAIATLQEHGLRHIGLGLFAPADDPLV
                     SARERDRLHLEIDGLSATPAAGTLAIGAGAFGRVGPACYRNAGGARPHAQAVESRGLS
                     VAAGLSLSTLGQARRSAVASLVCHGRLDFEAISLAYLVDFRHCFAGELGELQALARAG
                     LVELDEEGVELTWQGEHLVDTVLAVFDRCR"
     gene            4301..5035
                     /locus_tag="VIN75_18265"
     CDS             4301..5035
                     /locus_tag="VIN75_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8976165.1"
                     /translation="MSSPLPCAPLRPNVACSSCGLRELCLPVGFSDGEMADLDALVSA
                     RQTVRRGETLFRSGDRFDALYAVRTGFFKTSTSSEDGREHVTGFQMGGELLGLDGFDD
                     DTHRCDAVALEDSQVCVIPCAALETMTHRFPALQRQLHRVMSREIVHEHGLMMMLGGM
                     RAEERVAAFLANLARRLQARGFSPSAFVLRMTRQEIGTYLGLQLETVSRSFSRLHEAR
                     ILDVRQRNIRILDAAALQRLVAGGQD"
     gene            5041..7173
                     /locus_tag="VIN75_18270"
     CDS             5041..7173
                     /locus_tag="VIN75_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY8976166.1"
                     /translation="MSPVAATTLRIGGMHCAACADTVERAMRSVPGVVDAHVSAAAQA
                     AEVRWDPDVVGPEAIARAVHAAGYVATPDTTAAARAARKVESRTALWRLFVAALCATQ
                     VMMLAEPTYLAAPGEIDAEYTRLLNWGGWLLTLPVLLFSASPFFAGAWRSVRARRIGM
                     DVPVALGLAIAFVASTATTFGADAHAVYFDSIAMFVTFLLAGRWLEMRARHRAEESLE
                     ASTSQLPSTALRVDVSGNARRVPLEALAVGDRLRIPLGEAFPADGRLVDGATAADESL
                     LTGESHPVVKAAGDSLVAGSLNVGAPVDMLAERLGADTRAEAIAALMREARTQRPAFL
                     SPADRWAGLFLWIVLMLSAGAWLAWQVLDPARALDAAIAVLIVTCPCALSLAAPTALL
                     AAASRMGREGLLVRDMDAIARLAHVRTLFVDKTGTLTTGDQACSGLHALTAVPADELP
                     ALAAKASSLAAWSSHPLARAVNARWHPDLTVWSGVSEAAGKGVLALDEHGVEWRLGSA
                     AWTGGLPVPGADVVLARDGRPLVAFACGEIVRDDAAAAVRMLSQDGVEVRILSGDDPS
                     RVREVALALGIRDARGHQTPEDKLASLSAAQTGGRCVAMIGDGINDAPVLARADVAIA
                     MGSGAALARTRADAILLSNRLSDVVRALALARRTLRVVNQNLGWAALYNAACVPLALS
                     GHLPPWAAGLGMACSSLFVVLNSMRLGR"
     gene            7178..7363
                     /gene="ccoS"
                     /locus_tag="VIN75_18275"
     CDS             7178..7363
                     /gene="ccoS"
                     /locus_tag="VIN75_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY8976167.1"
                     /translation="MESLYLLVPLSALLVLALLGLFAWALDADQFDDLQREGERILAA
                     DESLDGDQPRGEAASKQ"
     gene            7404..>7930
                     /locus_tag="VIN75_18280"
     CDS             7404..>7930
                     /locus_tag="VIN75_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8976168.1"
                     /translation="MVNPSVATPPDTVYDDRAVRLFSIAAVIWGVVGMLVGVFIAAEL
                     AFPDIALGIPWLSYGRLRPLHTNAVIFAFGGCALMATSYYVVQRTCQVRLISSKLAEL
                     HFWGYQAVIVLAAITLPLGITRGKEYAELEWPIALLLTVVWVIYAIVFFGTIGIRKVR
                     HIYVANWFYGAFIIAV"
BASE COUNT         1111 a         2875 c         2785 g         1159 t
ORIGIN      
        1 catgctcggc acggcgttcc cgcccgcttt ccatcgcgtc gcgcggcagg ctctggctgg
       61 agcgggggaa catgaggaag acggtggtgc cgcgcccgac gacgctttcg atcttcacct
      121 cgccccctga ggaacggacg aagccgtaga cctggctgag gccgaggccg gtgccggtgc
      181 ccacgccttt ggtggtgaag aagggctcga acaccttctc gagcaattcg ggctcgatcc
      241 cctggccggt gtcgcggatg ctgagccgca ccatgtcgcg ccgcacctcg ccgcccgcgg
      301 gataattgtg cgcgccgatc cggatccggc cgccttcggg catcgcgtcg cgcgcgttga
      361 tcgcgacgtt gagcatggcc gactcgagct gcaccgggtc ggcgtagacc gcggggcacc
      421 ccggtggaca gtcgacctcg atgcgcacgc gctggtcgac cgtgcgccgc agcatgtcgg
      481 ccagggaatc gagcatgccg cgcaggtcga tggactcggg ctgcaacatc tgccggcgcg
      541 agaacgccag cagcttggcc gtcagctccg ctgcccggtg ggtggcgcgc cccgcggagg
      601 cgagcatggg gcgaacgtcg ccgactggtg tcgggccagg gctgtcctcg atgatctgca
      661 ggttgccctt gatcaccgtc agcaggttgt tgaagtcgtg cgcgatgccg ccggtcagct
      721 ggcccaccgt ctccagccgc tgggaatggc tcagcgcctc ctcggtgcgt ccgcgctgca
      781 ggcaggtggc cagcaggctg cacagcgatt cgacgaagcg ggtctcgtcc tcgccgaacc
      841 gagtcgacgc gcgggcgtgg atggtgagcg ttccgaagac gcggccgcgg tcgaccagtg
      901 gtgcggcgac gccgctgacg aaccccgcct ccagcaggtg cgccggcagc gcgaaccgct
      961 gctccaggcg gtagtccggc agcaggatcg tctgaggatg cgtgaccagg tagccgggca
     1021 gcgtggtcgg ctcgctggcc agcctgagcc cgacctgttg ctcggcgagc agcgcgtgcc
     1081 cgcccgcgat gacgaactcg cggccgcccg gggccagttc acagacgacg gcactgtgga
     1141 cgtccagcgc ctcgactgcc aggcggcacg cctgctccac cagcacgtgc atgtcgcgct
     1201 cgtcgaccgc caggcggccg agttgggcca ggcggtcgct gtaccgcgcc cgctgcaggg
     1261 cctgccgcac gcgcggatag gaccccacgt cgcggatcga cgcgaccacg aacggcagcc
     1321 cgtggtcctg cagcgggctc agcgcgatct ccaccaccac ctcgctgccg tcgccacggc
     1381 gggcgaccag atccatctgc gtgcccatgg gacgcggccg cggcgacttt ccgtaggcct
     1441 cgcggtaggc ggcgtgacgg ggccggatcg cgtccgggac cagttcgtcg acgctcatcc
     1501 gtgcgaactg gtcggcgctg tagcccagca gcgattcggc cgccgggttg ctcaggacga
     1561 tgttgcccgc ggcatcgacc agcagcatcg cgtccggatg ggcggagaag atgctgcgga
     1621 agacgctgcg ctcgtcgaag cctgacaacg ccaaggcggg agcttgttct cggcgcatgc
     1681 ggttcagccc cgggacgcag cggcttcttc cggcaagccg cggcgggggg atatccattt
     1741 catggcaagc ggcatgggaa ggacctcggg cttccgacgg gaacgacacc aggggtgccg
     1801 cgcacaggct cgagcgtacc tcggccgcgc cactttcgct gcggcaagac cgtgcgagcc
     1861 gcggcatcgg cgacgggcgc cctcatggcg tcggcctgga ctgatcgaga tcaagctcgc
     1921 tgcgcatcgg ccaggctaag gtgccgttgc cgcgcacgcc gatgtgcggc acgtcaacca
     1981 ggagagaccc agacatgacc acccgtcgcg ccttcatcca gatcctgccg ctcgccggcg
     2041 ccgccgccct gtgcgcgaat gccgcgctgg ccgccggggt cgccagcgtc gatcccaagg
     2101 atccgcaggc ggtcgcgctc ggctacgtgc cggacgccgc caaggccgac aaggccaagt
     2161 atccgaagta cgctgccggc caggcctgcg gcagctgcca gctgtaccag ggcaagccgg
     2221 ctgacgcggc ggccccgtgc gcgctgttcg gcggcaagca ggtcgccggc aaggcctggt
     2281 gcagcgccta cgtgaagaag gcctgacggg ggccgcgatg cgacccgccc tgcgtgtgct
     2341 gcgcgacgag cacgacgcgc tgtcggcatt gctgcacacg ctggtgctga tgctcgcgca
     2401 ggcccgggcg aaccatcggc cgcccgactt cggggccctg cgggccatgc tgttctacgt
     2461 cgacgagttc cccgagaagc gccatcaccg caaggagtcc gagctgctct ttccgcggct
     2521 gcgggcgcgc acaccgctcg cgcgcgacgt cctcgaccgc ctcgacgacg accacgagcg
     2581 gggtcacgcg cgcatccgcg acgtcgagca tgccctgacg gaatacgaga tgctcgggcc
     2641 cggccgcggg gcggacttcg agcgggcgct gcagcgatat gtcgacttct accgggccca
     2701 catggcgctc gaggaggacg agatattccc cctggcggaa cgccacctga ccgacgagga
     2761 ttggcaacac ctggaggccg aactcgcggg agagcccgat ccgctcaccg ggcgatcggc
     2821 cgacgaggag taccgggcct tgttcgaccg catcgtcggc ctggttccgg cgccgttcgg
     2881 actcgccgcc ccgagctcgc cgccctgacc ggcgggcgat tcagcggcag cgatcgaaga
     2941 ccgcgaggac ggtgtccacg agatgctcgc cctgccaggt cagttccacg ccctcttcgt
     3001 cgagctccac caggcccgca cgcgcgagcg cctgcagttc gcccagctcg ccggcaaagc
     3061 aatgccggaa gtcgacgagg tacgcgagcg agatcgcctc gaagtccagg cgcccgtggc
     3121 acaccagcga cgccaccgcg gatcggcgcg cctggccgag cgtcgacaac gagaggcccg
     3181 cagccaccga cagcccccgc gactcgacgg cctgcgcatg aggcctcgct ccgccggcgt
     3241 tccggtagca ggcgggcccg acccggccga aggcaccggc gccgatcgcg agcgtcccgg
     3301 cggcgggggt cgcggacaat ccgtcgatct ccagatgcag gcggtcacgc tcgcgggccg
     3361 agacgagcgg atcgtcggcg ggggcgaaga gccccaggcc gatgtgccgc aggccgtgct
     3421 cctgcagagt cgcgatcgcc cgggcgcgca gcacgccccg atcctcgaac agcgcgatcg
     3481 accctggagg cgtcggacat cgatcgcagg cggactcgtg cgtgctggcg tatggatgca
     3541 gatgcaccct cgtcgcgccg gcatgcagca tggcgccaag ctcttgcgcc agcaggtcgc
     3601 cgtcctggcc ggagcggccg caggcgaccc ggaccgtcac catcttgaag ccgagctcgc
     3661 gcgccgactc gacctccgcg gagcgtacgg cttcgatgac gaggctcgag acgccggcgg
     3721 cgcgccagga gcgcaacgcc gatgccccgg gccaccgagc ctcggcaacg acctcggcgg
     3781 cctccatccg gaactgcgcg cagatgcgat ccagcacatc cccgagtcgc ccgccggccg
     3841 cgcttgcgcc gcccaagccc tggacagtca ctcgtgtcac ctgctgcggc gccccgaccg
     3901 cgtcggagag cgcagccgcc tcccctgcca gcgcagacgc cgatcgcttc gcgtcgatgc
     3961 agccttcgca gggaaggcgg acggccaggt gcaacggcag ccgcctcgcc cgccctgggt
     4021 cctgccctcg ccgctcgagc gcctcgcgca actcgcctgc gccgaaggac ggcgagaagt
     4081 ccgcgaacgg cggcagcgcc ggatgccgcg acgcgccgcg atcgaatcgc ctcaacaagg
     4141 ctgcgggtgg cgccggagcg atggtctcga agggaaatga aggacgcatg accgcgatgc
     4201 tggggacacg ccgtctcccg attcttgatc cagcgcaacc gacctcgcct gcgtcgtccg
     4261 aacaatcgcc acattgcgcc aagtcagaaa cggaagctcc atgtccagcc ccttgccctg
     4321 cgccccgctg cggcccaacg tcgcctgctc cagctgcggg ctgcgcgaac tgtgcctgcc
     4381 cgtgggcttc agcgacggcg agatggccga cctggacgcg ctcgtgtcgg cgcgccagac
     4441 ggtgcgccgt ggcgagacgc tcttccgcag cggtgaccgc ttcgacgcgc tttacgccgt
     4501 gcgcaccggt ttcttcaaga ccagcacgtc cagcgaggac ggccgcgagc acgtgaccgg
     4561 cttccagatg ggcggcgaac tgctggggct ggacggcttc gacgacgaca cccaccgctg
     4621 cgacgccgtg gcgctcgagg attcgcaggt ctgcgtcatc ccctgcgcgg cgctcgagac
     4681 gatgacgcac cggtttcccg cgctgcagcg acagctccat cgcgtgatga gccgcgagat
     4741 cgttcacgag cacggcctga tgatgatgct gggcggcatg cgcgcggagg agcgtgtcgc
     4801 ggccttcctg gccaacctgg cccggcggct gcaggcgcgc ggcttctcgc cctccgcgtt
     4861 cgtcctgcgc atgacccgcc aggagatcgg cacctacctc gggctgcagc tggagacggt
     4921 cagccgcagc ttctcgcggc tgcacgaggc ccgcatcctc gacgtgaggc agcgcaacat
     4981 ccgcatcctc gacgcggcgg cgctgcaacg cctggtcgcc ggcggccagg actgaggcgc
     5041 atgtcgccgg tcgccgccac cacgctgcgc atcggcggca tgcattgcgc cgcctgcgcc
     5101 gacaccgtcg aacgggccat gcgcagcgtg ccgggcgtcg tcgacgcgca cgtgagcgcc
     5161 gcggcccagg cggccgaggt gcgctgggat ccggacgtcg tcggccccga ggcgatcgcg
     5221 cgggccgtgc atgccgccgg ctacgtggcc acgccggaca ccacggcggc ggcccgggcc
     5281 gcccgcaagg tcgaatcccg caccgcgctg tggcgcctgt tcgtcgccgc cctctgcgcg
     5341 acgcaggtca tgatgctggc cgagccgacc tacctggccg ccccgggcga gatcgacgcc
     5401 gagtacacgc gcctgctgaa ctggggcggt tggctgctga ccttgccggt gctgctgttc
     5461 tcggcgtcgc ccttcttcgc cggcgcctgg cgcagcgtgc gggcacggcg gatcggcatg
     5521 gacgtgcccg tcgcgctcgg actggccatc gccttcgtcg ccagcaccgc gacgacgttc
     5581 ggcgcggacg cgcacgcggt ctatttcgac tcgatcgcga tgttcgtcac cttcctgctg
     5641 gcggggcgct ggctggagat gcgcgccagg catcgcgcgg aagagtccct cgaggcatcc
     5701 acctcgcaac tgccgtccac ggcgctgcgc gtggacgtca gtggcaacgc ccggcgtgtg
     5761 ccgctggagg cgctggccgt cggcgaccgg ctgcgcatcc ccttgggcga ggccttcccg
     5821 gccgacggac gtctcgtgga cggcgcgacc gccgccgacg agtccctgct gacgggcgag
     5881 tcgcaccccg tggtcaaggc ggccggggat tccctggtcg ccggcagcct caacgtgggc
     5941 gccccggtcg acatgctggc cgagcgcctg ggggcggaca cgcgcgccga ggccatcgcg
     6001 gcactgatgc gtgaggcccg cacccagcgt cccgccttcc tgtcgccggc cgatcgctgg
     6061 gccggcctgt tcctgtggat cgtgctgatg ctgtccgccg gggcatggct ggcctggcag
     6121 gtgctggacc ccgcgcgcgc cctcgacgcg gccattgccg tcctgatcgt cacctgcccc
     6181 tgcgccctgt ccctggccgc gcccacggcg ctgctcgccg cggcgtcgcg catgggccgc
     6241 gaaggcctgc tggtgcgcga catggatgcg atcgcgcggc tggcgcatgt gcgcacgctg
     6301 ttcgtcgaca agaccggcac gctgaccacc ggcgatcaag cctgctccgg cctgcatgcg
     6361 ctgaccgcgg tgccggccga cgaactgccg gcactcgcgg caaaggcgtc gtcgctggcc
     6421 gcctggtcct cgcatccgct ggcccgggcg gtcaatgcgc gctggcatcc ggacctgacc
     6481 gtctggagcg gcgtgtccga ggctgccggc aagggcgtcc tggccctgga cgaacacggg
     6541 gtcgagtggc gcctcggttc cgcggcgtgg acgggcggcc tgcccgttcc tggcgccgac
     6601 gtggtgctgg cgcgcgacgg ccggccgctc gtcgcgttcg cctgcggaga gatcgtgcgc
     6661 gacgacgccg cggctgcagt gcgcatgctc tcgcaggacg gcgtcgaggt gcgcatcctg
     6721 tccggcgacg acccgtcccg cgtgcgcgag gtggcgctcg cgctcgggat ccgggacgca
     6781 cggggccacc agacgccaga ggacaagctc gcctcgttga gcgccgcgca gacgggcggc
     6841 cgttgcgtcg ccatgatcgg cgacggcatc aacgacgccc cggtgctcgc gcgcgccgac
     6901 gtcgcgatcg cgatgggctc gggcgccgcc ctcgcgcgga cacgggccga cgcgatcctg
     6961 ctgtccaacc ggctgtcgga cgtcgtgcgc gccttggcac tcgcgcggcg cacgctgcgc
     7021 gtcgtgaatc agaacctcgg ttgggcggcc ctctacaacg ccgcctgcgt gccgttggcg
     7081 ctcagcggcc acctgccccc ctgggccgcg ggcctgggca tggcgtgcag ctcgctgttc
     7141 gtcgtcctca attcgatgcg cctaggccgc tgacgcgatg gagagcctgt acctcctggt
     7201 tcccctgtcg gcgctgctgg tcctggcgct gctgggcctg ttcgcctggg cgctcgacgc
     7261 cgatcaattc gacgatctgc aacgcgaagg cgagcggatc cttgctgccg acgagtcgct
     7321 tgacggggat caacctcgcg gcgaggccgc ttcgaaacaa tgaccctgac accggcgagg
     7381 ccggtccgac atggagcaaa gcgatggtca atccttccgt ggcgacgcca cccgacacgg
     7441 tctacgacga ccgcgccgtg cgcctgttct cgatcgcggc ggtcatctgg ggcgtcgtgg
     7501 gcatgctggt gggcgtgttc atcgccgccg agctggcctt ccccgacatc gccttgggca
     7561 tcccctggct cagctacggc cgcctgcggc cgctgcacac caacgcggtg atcttcgcgt
     7621 tcggcggctg cgcgctgatg gccaccagct actacgtggt gcagcgcacg tgccaggtgc
     7681 ggctgatctc gtccaagctg gccgaactgc acttctgggg ctaccaggcg gtgatcgtgc
     7741 tcgccgcgat cacgctgccg ctgggcatca cgcgcggcaa ggaatacgcc gaactcgagt
     7801 ggccgatcgc cctgctgctg acggtcgtgt gggtgatcta cgcgatcgtc ttcttcggca
     7861 ccatcggcat ccgcaaggtg cgccacatct acgtggccaa ctggttctac ggcgccttca
     7921 tcatcgcggt
//
LOCUS       DATCRO010000671         7810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_232276, whole genome shotgun sequence.
ACCESSION   DATCRO010000671 DATCRO010000000
VERSION     DATCRO010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232276"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..3041
                     /gene="glnE"
                     /locus_tag="VIN75_18285"
     CDS             282..3041
                     /gene="glnE"
                     /locus_tag="VIN75_18285"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY8976169.1"
                     /translation="MNAPETPLPLSRGKALAAHSRFVQRVRRRHADMLDRLPPGLPDF
                     AGITALIAGLEADGVPVMSALRIARQLVLERLAVLDVEQHADQSEITAAMTHLAEATL
                     DRAHAQALADIDPRWGVPRNAAGERVEFWIIGMGKLGARELNVSSDIDLIYVYEEEVA
                     TDGEKPVSAQEYFGQVARRMYALIGDVTDEGFVFRVDLALRPNGNSGPPVVSLAMLEE
                     YFLVQGREWERFAWLKSRVVAPRSAVTSGRALALRSLVTPFVYRRYLDYGVFEGLRQL
                     HHKIREEAQRRAAGRPERANDVKLSRGGIREIEFIVQLLLVVRGGQFPEIRTRSTLKA
                     LEKLRATGLMKAETAARLHDAYVFLRRVEHRIQFLDDRQTHLLPTDDDDLRWIAASLG
                     LSCGKNACELLDRLGETREFVSTEFDTLLHDGRAPPPRNGGCRNGSCAAPQAQLDSES
                     WLDRLPEELGERVRRFADNPRVQNLREESRQRIAKLVQRAVEEIRSGNCTIVAATRFV
                     DWIEPLLRRESYLALLVERPNVQRRLLRLLGLARWPVRYLMLHPGVIDELADERTMIE
                     RFDRVALTAELEDRHEAWKRSGEADEGAMLDTLRRAHHAEVFRTLVRDVEGRITVEAV
                     ADELSALADTIVERSLQWAWTLMRKRHRDVPRLAVIAYGKLGGKELGYGSDLDVVFLY
                     DDAGEADPYQAQEVYAAFVRKLITWLTLRTAAGELFDIDTALRPNGNSGLLVTSIESF
                     ESYQRGRGSNTAWTWEHQAITRARFCAGDAAIGERFEATRRAVIEAERDADSLRQEIA
                     AMRQKVRDAHPQRDQRIDLKHGEGGLMDIEFAVQYLVLAHGRDHPGLQDNLGNIALLK
                     RAEEARLLDVGIGTAAADAYRELRRAQHRARLDEQPTLFPEETLAPERAAGRALWRAV
                     FGPGA"
     gene            3038..3877
                     /locus_tag="VIN75_18290"
     CDS             3038..3877
                     /locus_tag="VIN75_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976170.1"
                     /translation="MKALRIGGMAWVLLAALLCAGAVAVTVLSLDGGAAPIASPFALA
                     LDWQPALGLRETWRLWTSAWVHWSVAHLLVNAAGAAVVAFVGWRARLPAAAALGWLAA
                     WPLTQAFMAAVGAERLAVLMPHYGGLSGVLHAGAIVLGLSLAWPPERANAPARPRVTP
                     APADNGFSATHASAIEPSRITEGPWAMTEMGTATALPASTLDQPPAETATGDGSLRAR
                     WIGVAIVVGTVVKVAMEAPWQLTPHASAALGIAVAPVAHACGIVAGILAWAGARVVGG
                     WQR"
     gene            4061..4732
                     /locus_tag="VIN75_18295"
     CDS             4061..4732
                     /locus_tag="VIN75_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8976171.1"
                     /translation="MITLSGFTLSNYYNKVKMVLLEKGLPFVEEHCPMGGDKTEEMLM
                     CSPLGKVPYIRTEQGSLCESEVIVEYLEALQPEPRLMPADIWGQAKVRELVTFVELHL
                     ELVVRELYPEAFFGGKVSDANKARVQKLLARNIAAFQRLARFGPYLAGPDFTLADCAA
                     FVTLPLVAMGSKIIYGEDMLQSAGVDWKSYIKLIEQRPSAQKVTADRKRDQEAAKAGR
                     VAAAS"
     gene            complement(4763..5539)
                     /locus_tag="VIN75_18300"
     CDS             complement(4763..5539)
                     /locus_tag="VIN75_18300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8976172.1"
                     /translation="MFRDLFRMLRAAASALVGVALGLALPTTSSAMSSTKAIFFSSGI
                     QQRLEVAIRSDDAPSIDQLLAAGAQVNARGLHDVTPLMIAVDAQSPRAVAALLRAGAN
                     PNLKAADRAGAVHLAVQSRWAKPNGHDILAMLMKAGGDPNTRRPDGDPVLVSLTYDHD
                     LESIRWFKSLGADLDIIGRDDEPIIAGQAYAQNWDVVWVMIELGARYDYENTVFPLSK
                     AINGSYGSSPDSTLYSYKLRTWQLFKDHGFAVRPFGTDKH"
     gene            complement(5604..6857)
                     /locus_tag="VIN75_18305"
     CDS             complement(5604..6857)
                     /locus_tag="VIN75_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976173.1"
                     /translation="MGSFSRAGSASGKAPCVACCRADHAKDDELSKASTARIAALTGG
                     RVPPNAAPAPSATADTPSARVNSDCAQQARTLAPIVARFDRYREALPSARAAADLNEL
                     GDKSGDEPSGKPCLTRLPVDAERLNAALGMPPDAIKPLQLRNDETGFRAALYRDEGTG
                     RMILVPRDTQPDSLVDWQANTRNGLGLDTPQYAAMRKLTERLVANDQTFDLAGYSKGG
                     GIAQEGGLMNTLAQVRVFNSAGLADSSLSRTGQPNFESLVSRTRAFSSEGDFLTFMNN
                     TTDPGQNIINSRFLRQELAGQGPGLNPINIKVRNPAMRGANDAQFGRDKQAYIDELDQ
                     HIESMQTAFDSGGVVQSFPPVRAASKETIADSMTLAGKMLGARSDQPTLGKLAQHKMG
                     VVLDSMEANVANDRSALNAFLAKCG"
     gene            complement(6859..7233)
                     /locus_tag="VIN75_18310"
     CDS             complement(6859..7233)
                     /locus_tag="VIN75_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976174.1"
                     /translation="MLHFDTAQMSAITQAHFFSRVAEFIRDQTTVAAYRQAAMDTSLR
                     TALWAPHWPTLRNASEHDAALFMCFLLACDTLGVDSTRAAEAVRQSSQPETSMKLFLS
                     ERGLLRFSAFDVPDLTRPGAEA"
     gene            7564..>7810
                     /locus_tag="VIN75_18315"
     CDS             7564..>7810
                     /locus_tag="VIN75_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976175.1"
                     /translation="MPSSKTPAPAKKTRSQVAAAPPAAAVPVVAQEGAGRYLGSAMRA
                     AHPEAHAIRVRGARTHNLKNIDLDIPKHALVVITGLSG"
BASE COUNT         1166 a         2692 c         2773 g         1179 t
ORIGIN      
        1 gcgagcacga ccaggcacag gaaggacagg cccgcataga cgacggcttt ccaggccagt
       61 tggagcccgc gccgccacgg catgaagcgc agccgcctga cgactggatc gaaaaatgcg
      121 ctcggttgca atctcatgaa atgttcggca cggggtggca gcgccccaga ccacagtgta
      181 ggtgggcaag atgagcgccg aagggcgcga cagggagtct ttgtcccctg ggcccgccct
      241 gaacttctgg taacaagcgc cctcgcggcg ccgcgccggc cgtgaacgcc cccgagacgc
      301 cgctaccgct atcacgcggc aaggccctgg ccgcgcacag ccgcttcgtg cagcgcgtgc
      361 gccgccgcca cgccgacatg ctggatcggc tgccgccggg cctgcccgac ttcgccggca
      421 tcacggcgct gatcgccggg ctcgaggccg acggcgtccc cgtcatgagc gcgctgcgca
      481 tcgcgcgcca gctggtgctg gagcgcctgg ccgtgctcga cgtcgagcag catgccgacc
      541 agtccgagat cacggccgcc atgacgcacc tggccgaggc cacgctcgat cgcgcgcacg
      601 cacaggcgct ggccgacatc gacccgcgct ggggcgtgcc gcgcaacgcg gccggcgagc
      661 gcgtcgagtt ctggatcatc ggcatgggca agctcggcgc gcgcgagctc aacgtgtcgt
      721 ccgacatcga cctgatctac gtctacgaag aggaagtggc caccgacggc gagaagccgg
      781 tgagcgcgca ggaatacttc ggccaggtgg cgcgccgcat gtacgcgctg atcggcgacg
      841 tcaccgacga gggcttcgtg ttccgcgtcg acctcgcgct gcggcccaac ggcaactcgg
      901 gcccgccggt ggtgagcctg gcgatgctgg aggagtactt cctcgtgcag ggccgcgagt
      961 gggaacgctt cgcgtggctc aagagccgcg tcgtggcgcc gcgttcggcg gtcaccagcg
     1021 gccgcgccct ggcgctgcgc tcgctggtca cgcccttcgt ctaccgccgc tacctcgact
     1081 acggcgtgtt cgaaggcctg cgccagctgc accacaagat ccgcgaggag gcgcagcgcc
     1141 gcgccgcggg ccggccggag cgcgccaacg acgtcaagct gtcgcgcggc ggcatccgcg
     1201 agatcgagtt catcgtgcag ctgctgctgg tggtgcgcgg cggccagttc cccgagatcc
     1261 gcacgcgctc gacgctcaag gcgctcgaga agctgcgcgc cacgggcctg atgaaggccg
     1321 agacggccgc gcgcctgcac gacgcctacg tgttcctgcg ccgcgtcgag caccgcatcc
     1381 agttcctgga cgaccggcag acgcacctgc tgcccaccga cgacgacgac ctgcgctgga
     1441 tcgccgccag cctcgggctg tcctgcggca agaacgcctg cgagctgctc gaccgcctgg
     1501 gcgagacgcg cgagttcgtg tccaccgagt tcgacacgct gctgcatgac ggccgcgcgc
     1561 cgccgccgcg caacggcggc tgccgcaacg gcagctgcgc ggcgccgcag gcccagctgg
     1621 acagcgagag ctggctcgac cgactgcccg aggagctggg cgagcgcgtg cgccggttcg
     1681 ccgacaaccc gcgcgtgcag aacctgcgcg aggagagccg ccagcgcatc gccaagctgg
     1741 tgcagcgcgc cgtcgaggag atccgctcgg gcaactgcac gatcgtcgcc gccacgcgct
     1801 tcgtcgattg gatcgagccg ctgctgcggc gcgagagcta cctcgcgctg ctggtcgagc
     1861 gccccaacgt gcagcgccgc ctgctgcgcc tgctgggcct ggcgcgctgg ccggtgcgct
     1921 atctcatgct gcacccgggc gtcatcgacg agctcgccga cgaacgcacg atgatcgagc
     1981 gcttcgaccg cgtggcgctg acggccgaac tcgaggaccg acacgaggcc tggaagcgct
     2041 ccggcgaggc cgacgagggc gcgatgctgg acacgctgcg ccgcgcccac cacgccgagg
     2101 tgttccgcac gctggtgcgc gacgtcgagg gccgcatcac cgtcgaggcc gtggccgacg
     2161 agctgtccgc gctggccgac accatcgtcg agcgcagcct gcagtgggcc tggacgctca
     2221 tgaggaagcg ccatcgcgac gtgccgcgcc tggcggtcat cgcctacggc aagctgggcg
     2281 gcaaggaact gggctatggc agcgacctgg acgtcgtgtt cctgtacgac gacgccggcg
     2341 aggccgaccc gtaccaggcg caggaggtct atgccgcctt cgtgcgcaag ctgatcacct
     2401 ggctcacgct gcgcacggcg gcgggcgagc tgttcgacat cgacaccgcg ctgcgtccca
     2461 acggcaactc ggggctgctg gtgacgtcga tcgagtcgtt cgagagctac cagcgcggcc
     2521 gcggcagcaa caccgcgtgg acgtgggagc accaggccat cacgcgcgcc cgtttctgcg
     2581 ccggcgacgc ggcgatcggc gagcgtttcg aggccacgcg ccgcgccgtg atcgaggccg
     2641 agcgggacgc cgactcgctg cgccaggaga tcgcggcgat gcgccagaag gtgcgcgacg
     2701 cgcatccgca gcgcgaccag cgcatcgacc tcaagcatgg cgagggcggc ctgatggaca
     2761 tcgagttcgc cgtgcagtac ctggtgctgg cgcacggccg cgaccatccg ggcctgcagg
     2821 acaacctggg caacatcgcg ctgctcaagc gcgccgagga ggccaggctg ctggacgtgg
     2881 gcatcggcac ggccgccgcc gacgcctacc gcgaactgcg ccgcgcgcag caccgcgccc
     2941 gcctcgacga acagcccacg ctgtttcccg aggagacgct ggcgcccgag cgcgccgcgg
     3001 gacgcgcgct gtggcgcgcc gtgttcggtc ctggcgcgtg aaggccctcc gcatcggcgg
     3061 catggcctgg gtcctgctgg cggcgctgct gtgcgccggc gccgtcgcgg tcacggtgct
     3121 gtcgctggac ggcggcgccg cgcccatcgc ctcgcctttc gcactggccc tggactggca
     3181 gccggccctc gggctgcgcg agacctggcg gctgtggacc agcgcctggg tgcactggag
     3241 cgtggcgcac ctgctggtca acgccgccgg cgcggcggtc gtcgccttcg tgggctggcg
     3301 ggcgcggctg ccggccgcgg cggcgctggg gtggctcgcc gcctggccgc tgacccaggc
     3361 cttcatggcc gcggtcggcg ccgagcgcct ggccgtcctc atgccgcact acggcggctt
     3421 gtccggcgtg ctgcatgccg gcgccatcgt gctggggctg tcgctcgcgt ggccgcccga
     3481 gcgcgcgaac gcccccgcgc gtccgcgcgt gacgccagcg cccgcggaca acggcttctc
     3541 cgccacgcac gcgtcggcga tcgagccctc gcgcatcacc gaggggccgt gggccatgac
     3601 cgagatgggc accgccaccg ccctgcccgc ctcgacgctc gaccaacctc cggccgagac
     3661 tgccacgggc gacggctcgc tgcgcgcgcg ctggatcggc gtcgcgatcg tcgtgggcac
     3721 cgtggtcaag gtggcgatgg aagccccgtg gcagctgacg ccgcatgcca gcgcggcgct
     3781 gggcatcgcc gtggcgcccg tcgcccacgc gtgcggcatc gtcgcgggca tcctggcgtg
     3841 ggcgggagcg cgggtcgtgg gcggctggca gcgctgaatg ccgccaaggg gcgctcgggg
     3901 tggcgctctc gtcaagcgcc aagagtcgtc tctccaaagc ggaaagcctc gctgtcacgg
     3961 acgcgccatc ggtgcgcagg gggccgccct acacttgcac ctgccatcgg cgggcgccgt
     4021 catgcccgca ttcctccacg tccacccgtc aggcctcgcc atgatcacgc tcagcggatt
     4081 cacgctctcg aactactaca acaaggtcaa gatggtgctc ctcgagaagg gcctgccctt
     4141 cgtcgaggag cactgcccga tgggcggcga caagaccgag gagatgctga tgtgctcgcc
     4201 gctcggcaag gtgccctaca tccgcacgga gcagggctcg ctgtgcgaga gcgaggtgat
     4261 cgtcgaatac ctcgaggccc tgcagcccga gccgcgcctg atgcccgccg acatctgggg
     4321 ccaggccaag gtgcgcgagc tcgtcacctt cgtcgaactg cacctggaac tggtggtgcg
     4381 cgagctgtac ccggaggcct tcttcggcgg caaggtgagc gacgccaaca aggcgcgcgt
     4441 gcagaagctg ctcgcccgca acatcgccgc cttccagcgc ctggccaggt tcggccccta
     4501 cctggccggc cccgacttca ccctcgccga ctgcgccgcc ttcgtcaccc tgccgctggt
     4561 ggcgatgggc agcaagatca tctacggcga ggacatgctg cagtcggccg gcgtcgactg
     4621 gaagagctat atcaagctga tcgaacagcg cccgagcgcg cagaaggtga cggcggatcg
     4681 gaagcgggat caggaggcgg cgaaggcggg gcgggtggcg gcggcgagct gatcagggcg
     4741 tgcgcgcact atcaagcagc tttcaatgct tgtcggtgcc gaacggcctg acggcgaatc
     4801 cgtggtcctt gaacagttgc caagtcctga gcttgtagga atacagggtc gagtcgggag
     4861 acgagccgta gcttccattg atggccttgc tcagcggaaa gactgtgttc tcgtagtcat
     4921 agcgcgcacc aagttcgatc atcacccaga cgacgtccca gttctgcgcg tacgcctggc
     4981 ccgcaatgat gggttcgtcg tctcggccga tgatgtcgag atcagcgcca agggatttga
     5041 accagcgaat gctctccagg tcgtggtcgt aggtgaggct cacgaggacg ggatcgccat
     5101 cgggccgccg cgtgttcggg tctccgccag ctttcatgag catcgcgagg atgtcgtggc
     5161 cattcggctt ggcccagcgg ctttgaactg cgagatgcac ggcgcccgcg cggtcggccg
     5221 ccttcaggtt cggattcgcg ccggcacgca gcaacgccgc aaccgcgcgc ggagattgcg
     5281 catcgacggc gatcatcaac ggtgtcacat cgtgcagccc ccgggcgttg acctgggcac
     5341 ccgcggcaag aagctggtcg attgaaggcg cgtcgtcgct gcgaatggcg acttccagcc
     5401 gttgctggat gcccgaggaa aagaagatcg ctttggtcga agacatggct gaggacgtgg
     5461 tgggcagggc aaggccgagg gcgacaccca caagagcgga cgctgcggcg cggagcattc
     5521 tgaacaggtc acggaacatc tggctgcgga ctcctttgca gtgcgaaagc ttgtcgtcga
     5581 gcgaaggtcg ggttggcgac gcgctaaccg cacttcgcca ggaaggcatt gagcgcgctg
     5641 cgatcgttcg ccacattcgc ctccatggag tcgagcacga cgcccatctt gtgctgtgcc
     5701 aacttgccca gcgttggctg gtcgctgcgc gcgccgagca tcttgcccgc cagggtcatg
     5761 ctgtcggcga tggtctcctt ggatgccgcg cggaccggtg gaaacgactg gacgacgcct
     5821 ccgctgtcga aggcggtctg catgctctcg atgtgctggt ccagttcgtc gatataggcc
     5881 tgcttgtccc gaccgaactg cgcatcgttg gcgcctcgca tggcaggatt gcgcaccttg
     5941 atgttgatcg ggttcagtcc cggcccctgc ccagccagtt cctggcgcag gaagcgtgag
     6001 ttgatgatgt tctgcccggg gtcggtggtg ttgttcatga aggtcagaaa gtcaccctcg
     6061 gaactgaagg ctcgggtgcg cgagacgagg ctttcgaagt tgggctgacc tgtccgggaa
     6121 agggaactgt cagccaagcc tgccgagttg aagacgcgca cctgcgccag cgtgttcatc
     6181 agcccaccct cctgcgcaat gccgccgcct ttcgaatagc cggcgaggtc gaaggtctgg
     6241 tcgttggcga cgagacgctc cgtcagctta cgcatggcgg cgtactgcgg cgtatccaac
     6301 cccagcccat tgcgcgtgtt cgcctgccag tcgacaagac tgtccggttg ggtgtcgcgc
     6361 ggcaccagga tcatgcgacc cgtgccctca tctctatata gagcagcgcg gaatccggtc
     6421 tcatcgttgc gcagctgcag gggcttgatc gcatccggcg gcatgcctag cgccgcgttc
     6481 agccgctccg catccaccgg caacctcgtc aagcacggct tgcccgacgg ctcatcgccg
     6541 gatttgtcgc ccaattcgtt caggtcggcc gccgcgcggg cggatggcag ggcctcgcgg
     6601 tagcggtcga agcgggcgac gatcggggcc agggtgcggg cttgttgcgc gcagtcgctg
     6661 ttgacgcgcg cgctcggcgt gtcggccgtg gccgacggtg cgggggccgc gttgggcggg
     6721 acgcggcccc cggtgagcgc cgcgatgcgg gcggtgctcg ccttggacag ttcgtcgtcc
     6781 ttcgcgtggt cggcgcggca gcaggcgacg cagggcgcct tgccggaggc cgatccggcg
     6841 cgcgagaagc tgcccatgtc aggcctccgc gcccgggcgc gtgaggtcgg ggacgtcgaa
     6901 ggcggagaag cgcagcaggc cgcgttcgga caggaacagc ttcatgctcg tttccggctg
     6961 cgaggactgg cgcaccgcct cggcggcgcg cgtggagtcg acgccgagcg tgtcgcaggc
     7021 gagcaggaag cacatgaaca gcgcggcgtc gtgttcggag gcgttgcgca gcgtgggcca
     7081 gtggggggcc cacagcgcgg tgcgcagcga ggtgtccatg gccgcctggc ggtaggcggc
     7141 gacggtggtc tggtcgcgga tgaactcggc cacgcgggag aagaagtggg cctgcgtgat
     7201 cgcggacatc tgggcggtat cgaagtgcag catggcggcg gcgaagcggt ttcggatgcc
     7261 gccatgttcg gtcggcgccg aaaaactgcc cggcgcattc ggccgggttt cggcccggtt
     7321 tgacgcgccg cgtccagccg gccgggcgcc acaggcgacg cctcggcggg tcatgcgccc
     7381 tggcgcggca ccgaggcacc ctcgcccgcc gccggcccgg tacatcggcc ctcgccctcc
     7441 gccgccgcct catgtgcggt gccgcacctt gcggtccagc agggggcccg cggttcgccg
     7501 cttctgcgga cgtgcaaggg cttgacggcc tgtttatcat ggtcggttgc ctgtcgattt
     7561 cccatgccca gctccaagac ccccgctccc gccaagaaga cccgctccca ggttgccgcc
     7621 gctccgccgg ccgcggccgt cccggtggtc gcgcaggagg gcgccggccg ctacctcggc
     7681 agcgccatgc gcgccgcgca tcccgaggcg cacgccatcc gcgtgcgcgg ggcccgcacg
     7741 cacaacctga agaacatcga cctggacatc cccaagcacg cgctggtggt catcaccggc
     7801 ctgtcgggct
//
LOCUS       DATCRO010000672         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_491171, whole genome shotgun sequence.
ACCESSION   DATCRO010000672 DATCRO010000000
VERSION     DATCRO010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491171"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VIN75_18320"
     CDS             complement(<1..179)
                     /locus_tag="VIN75_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8976176.1"
                     /translation="MARTWRTLMQSIVSPVISTSLYFVVFGAAIGSRMNAVVEGVSYG
                     AFIVPGLIMLTVLTQ"
     gene            complement(227..880)
                     /locus_tag="VIN75_18325"
                     /pseudo
     CDS             complement(227..880)
                     /locus_tag="VIN75_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(907..1980)
                     /locus_tag="VIN75_18330"
     CDS             complement(907..1980)
                     /locus_tag="VIN75_18330"
                     /inference="COORDINATES: protein motif:HMM:NF008607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY8976177.1"
                     /translation="MIRIGILGAARIAPRGIVTPANDLLGVEVTAVASRELERARNFA
                     AQHSIPLALGSYAELIARDDIDLVVIATPNDTHHPLARDALRAGRHVVVDKPFTVRVA
                     DARELVALARERGRLLSVFHNRRYDGDFLTLRKLLAEGALGRVVDVESRHDRWRPEVR
                     QRWRESAGPGAGLWFDLGPHLVDQALQLFGPPRAIALERALVRDGALADDWFQATLRY
                     DRLHVHLRASMLAAASAPRFTLHGTRGSFVKEGLDAQEDALKAGVRPTWPAQAGWGVD
                     PGRAAIVTRTDDGRAVETPVMLERGAHQGYFAAVAAAVRGAAPNPVPPEQALAVMELI
                     ELGIESARQGRELEWRIEGAPAR"
BASE COUNT          311 a          756 c          766 g          329 t
ORIGIN      
        1 ctctgggtca gcaccgtcaa catgatgagt ccgggcacga tgaaggcgcc atagctcacg
       61 ccttcgacga cggcgttcat ccgcgatccg atcgccgcgc cgaacacgac gaaatacagc
      121 gaggtcgaga tcaccggcga gacgatgctc tgcatcagcg tgcgccaggt gcgcgccatc
      181 tcgaagcggt agatggcctt gacggcgtgc aggttggcga tcacgacttg tcctccacca
      241 ccaccagctc gcccctgcgg atcacgccga tgcggtcggc catctcctcg gcctcctcga
      301 tgtagtgggt ggtgaggatg atggtggtgc cggcgtcgcg gagttcgcgc accagctgcc
      361 acatgtcgcg ccgcagctcg acgtcgacgc ccgcggtggg ctcgtccagg aacagcacct
      421 gcggctcgtg cgacagcgcc ttggcgatca tcacgcgccg cttcatgccg cccgacagcg
      481 tgcggatgcg gttgtccttc ttgtcccaca gcgacagcgc cttgagcgtc ttctcgatgt
      541 gcgcgggagc gggcgccttg ccgaacaggc cgcggctgaa ggacaccgtg tcccacaccg
      601 tctcgaacgc gtcggtggtg agctcctgcg gcaccaggcc gatgatggag cgcgacaggc
      661 ggtagtcgtc gacgatgtcg tgcccgtcga ccgtcacctg gccggagctc gccgtgacga
      721 tgccgcaaac gatgctgatc agcgtcgtct tgcctgcgcc gttggggccc agcagcgcga
      781 ggatctcgcc cttgcggatg tccagcgcga tgtcgtggag cgccgtgaag cccgacgcgt
      841 aggtcttgga gacgttcttg acggagagga tgggctgcat gggccgggag tatcccgcca
      901 ttgccgctat cgtgctggcg cgccctcgat tcgccattcg agctcgcggc cttgccgtgc
      961 gctctcgatc ccgagctcga tcagctccat caccgcgagc gcctgttcgg gtggcaccgg
     1021 gttgggcgcc gcgccgcgaa cggccgcggc gaccgccgcg aagtagccct ggtgcgcgcc
     1081 gcgctccagc atcaccggcg tctcgacggc ccggccgtcg tcggtgcggg tgacgatcgc
     1141 cgcccggccc gggtcgacgc cccagcctgc ctgcgccggc catgtcggac ggacgccggc
     1201 cttgagcgcg tcctcctgcg cgtcgagccc ttccttgacg aagctgccgc gcgtgccgtg
     1261 cagcgtgaag cgcggcgcgc tcgcggcggc cagcatgctc gcgcgcaggt gcacgtgcag
     1321 gcggtcgtag cgcagcgtcg cctggaacca gtcgtcggcc agcgcgccgt cgcgcacgag
     1381 cgcgcgctcc agcgcgatcg cacgcggggg gccgaacagc tgcagcgcct ggtcgaccag
     1441 atgcgggccc aggtcgaacc acaggccggc gcccggaccg gcgctctcgc gccagcgctg
     1501 gcgcacctcg ggccgccagc ggtcgtgacg cgactcgacg tcgaccacgc ggcccagcgc
     1561 gccctcggcc agcagcttcc tgagcgtgag gaagtcgccg tcgtagcgcc ggttgtggaa
     1621 caccgacagc agccgcccgc gctcgcgcgc cagcgccacc agctcgcgcg catcggccac
     1681 gcggaccgtg aacggcttgt cgacgacgac gtggcggccc gcgcgcagcg cgtcgcgcgc
     1741 cagcgggtgg tgcgtgtcgt tgggcgtggc gatgacgacg aggtcgatgt cgtcgcgcgc
     1801 gatcagctcg gcataactac ccagagcgag cgggatcgag tgctgggccg cgaaattcct
     1861 cgcgcgctcc agttcccggg atgccaccgc ggtgacctcg acgccgagca gatcgtttgc
     1921 gggggtcacg atgccgcgcg gtgcgatgcg tgccgcgccc aggatgccga tgcgtatcat
     1981 ggggcggcag tctcgcacga ctcgcagccc tgcggcacac tagggaccat gacgaaggca
     2041 gctttgatag gcgccaccgg gcgcatgggg acgagcatcg tgcgggtggc ggccgaaggc
     2101 gcgagtcccg tggatgtcgt cgccgcggtg gcctccgccg gcagcaaatc actgggtcgc
     2161 ga
//
LOCUS       DATCRO010000673         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_345170, whole genome shotgun sequence.
ACCESSION   DATCRO010000673 DATCRO010000000
VERSION     DATCRO010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345170"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VIN75_18335"
     CDS             <1..605
                     /locus_tag="VIN75_18335"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY8976178.1"
                     /translation="ATLCAAPPDAPAAPPVAATADTGPPVLAYDGDKLRAPTDYREWI
                     YATTGLDMSYSRINLPGHHMFDNVFVNPAAWQAFKRTGTWPDRTVMVLETRGANTKGS
                     INRRGYFQDADLMGVEVHVKDIARFGGDGWAFFTFEDTDKPSAMISRDNDCYSCHKAH
                     GAVDSTFVQFYPTMLSLAKAKGTLAASYLQDVARDKPGAQ"
     gene            778..1242
                     /locus_tag="VIN75_18340"
     CDS             778..1242
                     /locus_tag="VIN75_18340"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEY8976179.1"
                     /translation="MPTIKLDVPLVAQEKANCCWHTSAYMIWLYWQQNGKGAGPMNTV
                     ASKYEVADTTGLYPAEFITLAQKVGLYKLYPNAQISEADLFAFLRAGGPIWSAGYWFG
                     CGHIIVVTGVEKGEVFFNDPDQGVKKKGTLKWFNDKLARLPGCMMVKDPGAY"
     gene            complement(1330..>2118)
                     /locus_tag="VIN75_18345"
     CDS             complement(1330..>2118)
                     /locus_tag="VIN75_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976180.1"
                     /translation="GAAYAKALAMNRALQPPAVLAETVNLGNLARLQFRQGGRAEAEA
                     GLRDAMTRREHVLGADYRDNGRGYDRAWLADILLARGRLDEARTVAADALAEALAVHP
                     EASPDTAFALVVDARLAAASGDWRQAATRAAQAVAMDETLADEASERAIRARLQFGEI
                     LHRLGRDAEARSQLARALAAADAQAPAPPALVGHIAAELALVAASLGDARAAAGLRER
                     AQAALAAIPAGRNAERDKVVQLLVQGRDIRRIATSGSARRPASG"
BASE COUNT          317 a          760 c          741 g          300 t
ORIGIN      
        1 gggccacgct gtgcgccgcc ccgcccgatg cgccagccgc gccgccggtg gccgccaccg
       61 ccgacaccgg cccgcccgtg ctcgcctacg acggcgacaa gctccgggcg ccgaccgact
      121 accgcgaatg gatctacgcc acgaccggcc tggacatgag ctacagccgg atcaacctcc
      181 ccggccacca catgttcgac aacgtgttcg tgaaccccgc ggcctggcag gccttcaagc
      241 gcaccggcac gtggccggac aggacggtga tggtgctcga gacccgcggc gcgaacacga
      301 agggctcgat caacaggcgc ggctacttcc aggacgccga cctgatgggc gtcgaagtcc
      361 acgtcaagga catcgcgcgg ttcggcggcg acggctgggc cttcttcaca ttcgaggaca
      421 ccgacaagcc ctcggcgatg atttcccgcg acaacgactg ctacagctgc cacaaggcgc
      481 atggcgcggt cgactcgacc ttcgtccagt tctatcccac gatgctgtcg ctcgcgaagg
      541 ccaagggcac gctcgcggcc tcgtacctgc aggacgtcgc acgggacaag cccggggcgc
      601 agtgacgccc ggacgatggg cccggccggg ggtcttggct ggcaatggtc gatggcgccg
      661 ggacgtgccg cctggcgagg cgtcgggcgc gttcgggtgt tggggatacg cgccatcggc
      721 ccacggtgct agaaccgctg acgtcgattg cccgcagcac caggaaggat ccgcgccatg
      781 cctacgatca agctcgatgt gcccctcgtt gcccaggaaa aggccaactg ctgctggcac
      841 accagcgcct acatgatctg gctgtactgg cagcagaacg gcaaaggcgc cgggcccatg
      901 aacaccgtgg catcgaagta cgaggtcgcc gacacgacgg gcttgtatcc ggccgagttc
      961 atcacgctgg cgcagaaggt cgggctgtac aagctttatc cgaatgccca gatcagcgag
     1021 gcggacctgt tcgccttcct gcgcgccggc ggcccgatct ggtcggcggg ctactggttc
     1081 ggttgcgggc acatcatcgt ggtcacgggt gtcgagaagg gcgaggtctt cttcaacgac
     1141 ccggaccagg gcgtgaagaa gaagggcacg ctcaagtggt tcaacgacaa gctcgcgcgc
     1201 ctgccgggtt gcatgatggt caaggatcct ggcgcctact agggcctgtt cacacgaaat
     1261 gcgtgcccgc gccggctgcc cgaagctgcc gtccgtccat gtcaagccgt cgcggcggcg
     1321 attcccggac tatccgctcg cgggccgccg agccgacccg gacgtcgcga tgcggcggat
     1381 gtcgcgtccc tggaccagca gctggacgac cttgtcgcgc tccgcgttcc ttccagcggg
     1441 gatcgcggcc aacgcggcct gtgcgcgctc gcgcaagccg gccgcggccc gcgcatcgcc
     1501 gagcgacgcg gccaccagcg ccagttccgc cgcgatgtgg ccgaccagcg ccggtggtgc
     1561 gggcgcctgg gcatctgcgg ccgcgagggc gcgcgccagc tgcgacctcg cctcggcgtc
     1621 gcgcccgagc cggtgcagga tctcgccgaa ctggagccgc gcccggatcg cgcgctccga
     1681 cgcctcgtcg gccagcgtct cgtccatcgc gaccgcctgg gccgcgcgcg tcgccgcctg
     1741 ccgccagtcg ccgctggcgg cggccagccg ggcatcgacg accagggcga acgcggtgtc
     1801 cggactcgcc tcgggatgga cggccagcgc ctcggcgagc gcgtcggcgg cgaccgtgcg
     1861 cgcctcgtcc aggcgtccgc gggcgagcag gatgtcggcg agccaggcac ggtcgtagcc
     1921 gcggccgttg tcccggtagt ccgcgccgag gacgtgctcg cggcgcgtca tggcgtcgcg
     1981 caggcccgcc tccgcctcgg cacggccgcc ctgcctgaac tgcaggcgcg cgaggttgcc
     2041 gaggttcacc gtttcggcca ggacggcggg cggctggagg gcgcggttca tggccagcgc
     2101 cttcgcgtag gccgcccc
//
LOCUS       DATCRO010000674         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_885185, whole genome shotgun sequence.
ACCESSION   DATCRO010000674 DATCRO010000000
VERSION     DATCRO010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885185"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="VIN75_18350"
     CDS             <1..2041
                     /locus_tag="VIN75_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976181.1"
                     /translation="LILSDQLCKNPEGNLSPKEVEFSKTIHSSGNDLLMLINDILDLS
                     KIESGTVAVDASELRVDDLHRSVERGFRHFAESKHVDFVVELKHPLPKTMVTDVKRLQ
                     QIIKNLLSNAFKFTHEGQVTFSIATASGGWSVDNDELHRAGTVLAFSVKDTGIGISPD
                     KQQIIFEAFQQADGSTSRKYGGTGLGLAISRELSRLLGGEIRLVSEPGKGSEFTLYLP
                     QSYNPARNARHARASVAPATTGESNVVALNRARPVPAAPPPEGDDGWTYDETDKQASE
                     SAMFANVADDDRGAIATGDRVLLIVENDLAFARVLLAGARAAGFKGLVTSTGASALTM
                     AREFHPSVITLDIHLPDMSGWRILDRLKGDLVTRHVPICVVSTDDARDRALRSGALGF
                     LAKPLQSADEADAVLLQLHSYAERRTKRLAVVMPPSARRSTFIATLDTDVDVVLAEDR
                     ESARHALADADALVVEDTLADFGPEDVLDVIAERAGICQLPVVLYHGGAQETGNWNQR
                     RAGLTIAEAATLPELRMQTALWLHRSPSALSDADREAIETLLVSSCPLKGRRALIVDD
                     DMRNIFALATVLDEEGMVIVSADNGREAIRLVQEDETIDIVLMDIMMPEMDGIATMKE
                     IRKLPRGRELPMVAVTAKAMKGDRQKCIEAGAWDYISKPVDPRYLMSVLRGWLCR"
     gene            2026..>2870
                     /locus_tag="VIN75_18355"
     CDS             2026..>2870
                     /locus_tag="VIN75_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976182.1"
                     /translation="MAVPLTATSPAALVPPSSLFGGLDDPADRTDILVVDDLPEKLLV
                     FETVLGELNQNIVFARSGSDALRQILRRDFAVILLDVNMPDMDGFETAALIRRYKRAA
                     HTPIIFITSYADEMQTAKGYSLGAVDYIPSPVVPEVLRSKVRVFVELHILQRRIARQA
                     EERVALAASEAALRLAEESTKRSSLLAGLSHALSGVLDPRESMHVLLAHVAPALAPNA
                     TVALVDDRGVVTHAATRTSLFEDDAQSVEIAPSALPSAHFALLRRALERPVEAIEPAS
                     PFVDGA"
BASE COUNT          456 a         1034 c          980 g          400 t
ORIGIN      
        1 gctgatcctg tcggaccagc tgtgcaagaa ccccgagggc aacctgtcgc ccaaggaagt
       61 ggagttctcc aagacgatcc actcgtcggg caacgacctg ctgatgctga tcaacgacat
      121 cctggacctg tccaagatcg aatcgggcac ggtggcggtc gacgcgagcg agctgcgcgt
      181 ggacgacctg caccgctcgg tggaacgcgg cttccgccac ttcgccgagt ccaagcacgt
      241 cgacttcgtc gtcgagctca agcacccgct gcccaagacg atggtcaccg acgtcaagcg
      301 cctgcagcag atcatcaaga acctgctgtc caacgcgttc aagttcacgc acgagggcca
      361 ggtcaccttc agcatcgcca cggcgagcgg cggctggtcg gtcgacaacg acgagctgca
      421 ccgcgccggc accgtgctcg cgttctcggt caaggacacc ggcatcggca tctcgcccga
      481 caagcagcag atcatcttcg aggccttcca gcaggccgac ggctcgacgt cgcgcaagta
      541 cggcggcacg ggcctgggcc tggcgatcag ccgcgagctc tcgcgcctgc tgggcggcga
      601 gatccgcctg gtcagcgagc cgggcaaggg cagcgagttc acgctgtacc tgccgcagag
      661 ctacaacccc gcgcgcaacg cgcgtcacgc ccgcgcgagc gtcgcgcctg cgacgaccgg
      721 cgagtccaac gtcgtcgcgc tgaaccgcgc gcgtccggtg cccgccgcgc cgccgccgga
      781 gggcgacgac ggctggacct acgacgaaac cgacaagcag gccagcgaat cggcgatgtt
      841 cgccaacgtc gccgacgacg accgcggcgc catcgccacc ggcgaccgcg tcctgctgat
      901 cgtcgagaac gacctggcgt tcgcgcgcgt gctgctggcc ggcgcccgcg ccgcgggctt
      961 caagggactg gtcacgagca ccggcgcgag cgcgctgacg atggcgcgcg agttccaccc
     1021 gtcggtgatc acgctcgaca tccacctgcc cgacatgtcc ggctggcgca tcctcgaccg
     1081 cctcaagggc gacctggtca cgcgccacgt cccgatctgc gtggtctcca ccgacgacgc
     1141 ccgcgaccgc gcgctgcgct ccggcgcgct cgggttcctc gccaaaccgc tgcagtcggc
     1201 cgacgaggcg gacgccgtcc tgctgcaact gcacagctac gccgagcgcc gcaccaagcg
     1261 cctggcggtc gtcatgccgc cgtcggcgcg ccgcagcacg ttcatcgcca cgctggacac
     1321 cgacgtcgac gtcgtcctcg ccgaggatcg cgagtcggcc cgccatgcgc tggccgacgc
     1381 cgacgcgctg gtcgtcgagg acacgctggc cgacttcgga ccggaggacg tgctggatgt
     1441 catcgccgag cgtgcgggca tctgccagct gccggtggtg ctgtaccacg gcggcgcgca
     1501 ggagaccggc aactggaacc agcgtcgcgc cggcctgacg atcgccgagg ccgccacgct
     1561 gcccgagctg cgcatgcaga ccgcgctgtg gctgcatcga agtccgtcgg cgctgtccga
     1621 cgccgaccgc gaggcgatcg agacgctgct cgtgagcagc tgcccgctca agggccgcag
     1681 ggcgttgatc gtcgacgacg acatgcgcaa catcttcgcg ctggccaccg tgctggacga
     1741 ggagggcatg gtgatcgtgt cggccgacaa cggccgcgag gcgatccggc tggtgcagga
     1801 ggacgagacg atcgacatcg tcctgatgga catcatgatg ccggagatgg acggcatcgc
     1861 caccatgaag gagatccgca agctgccgcg cggccgcgag ctgccgatgg tggcggtgac
     1921 cgccaaggcg atgaagggtg accgccagaa atgcatcgag gccggcgcgt gggactacat
     1981 ctccaagccg gtcgacccgc gctacctgat gtccgtgctg cgggggtggc tgtgccgctg
     2041 accgcgacgt ccccggccgc gctcgtgccg ccgtccagcc tgttcggcgg gctggacgac
     2101 ccggccgacc gcaccgacat cctggtggtc gacgacctgc ccgagaagct gctcgtgttc
     2161 gagacggtgc tgggcgaact gaaccagaac atcgtcttcg cgcgctcggg ctcggacgcg
     2221 ctgcgccaga tcctgcggcg cgacttcgcg gtcatcctgc tggacgtcaa catgccggac
     2281 atggacggct tcgagacggc cgcgctgatc cgccgctaca agcgcgccgc gcacacgccc
     2341 atcatcttca tcacgtcgta cgccgacgag atgcagacgg ccaagggcta ctcgctgggc
     2401 gcggtcgact acatcccgtc gccggtggtg cccgaggtgc tgcgctccaa ggtgcgcgtg
     2461 ttcgtggagc tgcacatcct gcagcgccgc atcgcgcgcc aggccgagga gcgcgtggcg
     2521 ctcgcggcgt cggaggccgc gctgcggctg gccgaggaat cgaccaagcg ctccagcctg
     2581 ctggcgggcc tgagccacgc gctcagcggc gtcctcgacc cgcgcgagag catgcacgtg
     2641 ctgctcgcgc acgtggcgcc ggcgctggcg cccaatgcca cggtggcgct cgtcgacgac
     2701 cgcggcgtgg tcacgcacgc ggccacgcgc acgagcctgt tcgaggacga tgcccagagc
     2761 gtcgagatcg cgccgtccgc gctgccgagc gcgcatttcg cgctgctgcg ccgggcgctc
     2821 gagcggcccg tcgaggcgat cgaaccggcg tcgcccttcg tcgatggcgc
//
LOCUS       DATCRO010000675         5143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_108725, whole genome shotgun sequence.
ACCESSION   DATCRO010000675 DATCRO010000000
VERSION     DATCRO010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108725"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VIN75_18360"
     CDS             complement(<1..815)
                     /locus_tag="VIN75_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976183.1"
                     /translation="MTAFTLAAADSFPAADLHAANLAAFADYIAGPMQLSLDQWPSLI
                     ARQGIDLGRSRVAVRDGAIASYAFVCPRPESGRWRLGIMGALPAERGSGAAPVLIEDF
                     LARAKAEGMAHAELECFAANERALRLYRRHGFEVAFPLNGWKQPADGAPALARDAPAG
                     EVREVDRATAFAWLADADRRVAWLPFQNTDRCQAAQVRPLTAWQRGSALMLFSVLEGT
                     PTHIHVLVDLDPGLRDAETLARAIRAAHPDAFAPPILRDDLGGEALARAGFVR"
     gene            complement(829..4092)
                     /locus_tag="VIN75_18365"
     CDS             complement(829..4092)
                     /locus_tag="VIN75_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8976184.1"
                     /translation="MFLSDFSIKRPVAAIVIIITLMGLGLIALNKLRVNQIPDVEQPV
                     LVVGITYPGASPDTVEREIINRVERALQGIEGVDQSRSSSTASEGYAQFVLVFEFHKD
                     MSLASDEVRNAIGTVRYKLPTEMREPVLTRVDPSAQPIMQMALSSPTLSHAEISRLAE
                     DQLADRFRALPGVATVTVNGSLTRELSVLLHAQKLREFGVSVTDVVNALRAQNATAPV
                     GKVRGALEDQSIRLVGRFESPAEFNQMLVKRSGDNLIRLGQVATVQDGFAELGGYSEH
                     NGTPNVGISVTRTRDGSTVTIAKEVRELVTTINKEQAGKAKLDVTQDGGNDAQDSLDN
                     VVDALFFGAGLTILVVYVFLNSWRSTLITGLSLPTSVITAFIAVWLCGFTLNFMSLLG
                     LSLAIGVLIDDAIVVRENIVRHMQRGEDRRTAALNGTAEIGMAVASTTFSIIAVFAPV
                     AFIPGITGEWFRPFGLTVVASVAVSLFISFTLDPMLSAYWGDPVDLHSAPRKGLGAVL
                     QRFNDWFDHQADRYGRIIAWALHHRWYMGWIAVLSFVGALVLQGAAGGSDFLPKSDSG
                     TLAIDIRMPPSSSLDYARMKLEEAQAVARQLPETVATNSWVNPNQGRVYVDIGKSTKR
                     KRSASEVGDDLRARMGRLVGAEFVVLDDLNNGAQKPVQIRFHGTDSRQLLAITNDFMD
                     KLRKVKGAVDVGLSEQDPQQELRIDLDRGLANSMGVSVNDAAQSLRVAFAGVEAGDWI
                     DPTGEARDVAVRLAPEDRVDASNIERLPIGIAGTDRTVPLEQIATVTMGKAPSKIQHA
                     NSKRMMVVAANAQGRSSGEVTADALKIAKAIQFPPGFGIELAGSAQSQQEVFSNLFIA
                     LVSGVLLMYLVLVMQFGSFTAPIPVMISLPLSLIGVVIALVSTHGTLNLMSIIGVIML
                     MGLVAKNAILLLDAARAEEREGAEREDALMHAGRKRLRPILMTTFALIAGMLPVAIGM
                     GEGGEFYRPMAVTIIGGTITSTMLTLLVIPCFYDSIEMMRERMLARLPHRSERFTAVG
                     AVLLTVLEFFAFLLCLRFLYRLVRRALGLPTGGLWRRPEDEHVAPPMPHSSEPMFNP"
     gene            complement(4106..>5143)
                     /locus_tag="VIN75_18370"
     CDS             complement(4106..>5143)
                     /locus_tag="VIN75_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976185.1"
                     /translation="APSATLLIAPEDVRTVGLQTHASGPVVTGSVEPERRADLRAEVS
                     AVVLQVLKENGEPVRKGDLLVRLDDTSIRDSLRSAEEASRAAAQALDQAQRQFTRVKT
                     LQAEGMSSQQALDDAEVRRNSAQSDRVAADSRTVAARQQLARTEVRAPFDGVVSDRKV
                     SAGDTAAIGKELLKVIDPASMRFEGLVSADRMGDIHAGQAVQFRINGFDKGDFTGKVR
                     HVDASADPVTRQVAVIVDFAPGTAPKVAGLYAEGRISSGASQALLLPEATIVKEGDKA
                     YVWRLGADAIRKVPVTLGERDARLGNVVILAGIAAGDRLLRTPGSTLVDGQKFEFAKP
                     AASAASVAPGA"
BASE COUNT          768 a         1772 c         1846 g          757 t
ORIGIN      
        1 tgccgcacga acccggcgcg ggcgagcgcc tcgccgccga gatcgtcacg caggatcggc
       61 ggcgcgaacg cgtccggatg cgcggcgcgg atggcgcgcg ccagcgtctc ggcatcgcgc
      121 aaccccggat cgaggtcgac cagcacatgg atgtgcgtgg gcgtgccctc gagcacgctg
      181 aacagcatca gcgcactgcc gcgctgccag gccgtcagag gccgcacctg cgccgcctgg
      241 cagcggtcgg tgttctggaa cggcagccac gcgacgcggc ggtcggcgtc ggcgagccag
      301 gcgaacgcgg tggcgcggtc gacctcgcgc acctcgcccg cgggggcgtc acgcgcgagc
      361 gccggcgcgc cgtcggccgg ctgcttccag ccgttgagcg gaaacgcgac ctcgaagcca
      421 tggcggcggt acaggcgcag cgcacgctcg ttcgccgcga agcactcgag ttcggcgtgc
      481 gccatgccct cggccttcgc acgcgcgagg aagtcctcga tcagcacggg tgcggcgccg
      541 ctgccgcgct cggcgggcag cgcccccatg atgcccaggc gccagcggcc ggactccggg
      601 cgtgggcaca cgaacgcgta cgacgcgatg gcgccgtcgc ggaccgccac ccggctgcgc
      661 cccaggtcga tgccctggcg cgcgatcagc gagggccact ggtccagcga cagctgcatc
      721 gggccggcga tgtagtcggc gaaggccgcg aggttggccg cgtggaggtc ggccgccggg
      781 aagctgtccg cggcggcgag cgtgaaggct gtcatcggtg ccggtggatc aggggttgaa
      841 catcggctcc gacgaatgcg gcatcggcgg cgccacgtgc tcgtcctcgg gccggcgcca
      901 caggccgccg gtgggcaggc ccaaggcgcg gcggacgagg cggtacagga agcgcaggca
      961 cagcaggaag gcgaagaact ccagcaccgt gagcagcacc gcgccgaccg cggtgaagcg
     1021 ctccgagcga tgcggcaggc gcgccagcat gcgttcgcgc atcatctcga tcgagtcgta
     1081 gaagcacggg atcacgagca gcgtcagcat cgtcgacgtg atggtgccgc cgatgatggt
     1141 gaccgccatc gggcgataga actcgccacc ctcgcccatg ccgatggcca ccggcaacat
     1201 gccggcgatc agcgcgaagg tcgtcatcag gatcgggcgc aggcgcttgc ggccggcgtg
     1261 catcagcgcg tcctcgcgct cggcgccttc gcgctcctcg gcgcgcgcgg cgtcgagcag
     1321 caggatggcg ttcttggcca ccaggcccat cagcatgatg accccgatga tgctcatgag
     1381 gttgagcgtg ccgtgcgtgg acaccagcgc gatcaccacg ccgatcaggc tcagcggcag
     1441 cgagatcatc acggggatgg gtgccgtgaa cgagccgaac tgcatcacca gcaccaggta
     1501 catcagcagc acgccggaga ccagcgcgat gaacaggttg ctgaacacct cctgctggct
     1561 ctgcgcggag ccggccagct cgatgccgaa gcccggcggg aactggatgg ccttggcgat
     1621 cttgagcgcg tcggcggtga cctcgcccga cgagcggccc tgggcgttgg cggccaccac
     1681 catcatgcgc ttggagttcg cgtgctggat cttggaaggc gccttgccca tcgtcacggt
     1741 ggcgatctgc tccagcggaa cggtgcggtc cgtgccggcg atgccgatcg gcaggcgctc
     1801 gatgttgctc gcgtcgacgc gatcctcggg cgcaagccga acggccacgt cccgcgcctc
     1861 gccggtgggg tcgatccagt cgcccgcctc gacgcccgcg aaggccacgc gcagcgactg
     1921 cgccgcatcg ttgaccgaca cgcccatcga gttggccagg ccgcggtcga ggtcgatccg
     1981 cagttcctgc tgcggatcct gctcggacag gcccacgtcg accgcgccct tgaccttgcg
     2041 cagcttgtcc atgaagtcgt tggtgatggc cagcagctgg cgcgagtcgg tgccgtggaa
     2101 gcggatctgc accggcttct gcgcgccgtt gttcaggtcg tccagcacga cgaactcggc
     2161 gccgacgagg cggcccatcc gcgcccgcag gtcgtcgccg acctcgctgg cactgcgctt
     2221 gcgcttggtg ctcttgccga tgtcgacgta gacgcggccc tggttgggat tgacccacga
     2281 gttggtggcc accgtctcgg gcagctggcg cgcgacggcc tgcgcctcct cgagcttcat
     2341 gcgagcgtag tccaggctgg agctgggcgg catgcggatg tcgatcgcca gcgtgccgct
     2401 gtcggacttg ggcaggaagt cggagccgcc ggccgcgcct tgcagcacga gcgccccgac
     2461 gaagctgagc acggcgatcc agcccatgta ccagcgatgg tgcagcgccc aggcgatgat
     2521 gcggccgtag cggtcggcct ggtggtcgaa ccagtcgttg aagcgttgaa gcacggcgcc
     2581 caggcccttg cggggcgcac tgtgcaggtc gacgggatcg ccccagtagg ccgacagcat
     2641 cgggtcgagc gtgaaggaga tgaacaggct cacagccacc gacgccacca cggtcaggcc
     2701 gaacggccgg aaccattcgc cggtgatgcc cgggatgaac gcgaccggcg cgaagaccgc
     2761 gatgatggag aaggtggtcg aggccaccgc catgccgatc tcggccgtgc cgttgagcgc
     2821 cgcggtgcgc cggtcttcgc cgcgctgcat gtggcgcacg atgttctcgc gcaccacgat
     2881 cgcgtcgtcg atcagcacgc cgatggccag cgacagcccc agcaggctca tgaagttgag
     2941 cgtgaagccg cacagccaga cggcgatgaa ggcggtgatg accgaggtgg gcaggctcag
     3001 gcccgtgatc agcgtcgagc gccacgagtt gaggaacacg tagaccacga ggatggtcag
     3061 gccggcgccg aagaacagcg cgtcgaccac gttgtcgagg ctgtcctgcg cgtcattgcc
     3121 gccgtcctgg gtgacgtcca gcttggcctt gccggcctgt tccttgttga tcgtcgtgac
     3181 cagctcgcgc acttccttgg cgatggtcac cgtgctgccg tcgcgcgtgc gcgtgaccga
     3241 gataccgacg ttgggcgtgc cgttgtgctc gctatagcct cccagctcgg cgaagccgtc
     3301 ctgcacggtg gcgacctggc ccaggcgaat gaggttgtcg ccgctgcgct tgaccagcat
     3361 ctggttgaat tccgccggcg actcgaagcg gccgacgagg cggatgctct ggtcctcgag
     3421 cgcgccgcgc accttgccga ccggcgcagt ggcgttctgc gcccgcagcg cgttgacgac
     3481 gtcggtgacc gacacgccga actcgcgcag tttctgcgca tgcagcagca cgctcagctc
     3541 gcgcgtgagc gagccgttga cggtgaccgt ggccacgccc ggcagcgcgc ggaagcggtc
     3601 ggccagctgg tcttccgcga ggcgcgagat ctcggcgtgc gacagcgtcg gcgacgacag
     3661 cgccatctgc atgatgggct gcgcgctcgg gtcgacgcgc gtgagcacgg gctcgcgcat
     3721 ctcggtgggc agcttgtagc gcacggtgcc gatcgcgttg cgcacctcgt cggaggccag
     3781 cgacatgtcc ttgtggaact cgaacaccag cacgaactgc gcgtagccct cgctggccgt
     3841 ggacgacgag cgcgactggt cgacgccctc gatgccctgc agcgcgcgct cgacgcggtt
     3901 gatgatctcg cgctcgaccg tgtcgggcga cgcgccgggg taggtgatcc cgaccacgag
     3961 caccggttgc tcgacgtcgg gaatctggtt gacccgcagc ttgttcagcg caatgagacc
     4021 caatcccatc agcgtgatga tgatgacgat cgcggccacc ggccgcttga tgctgaagtc
     4081 ggagagaaac atcgtcgcct cccgatcagg cgccgggcgc cacggacgcg gccgacgcgg
     4141 ccggcttggc gaactcgaac ttctggccgt cgaccagcgt ggaacccggc gtgcgcagca
     4201 ggcggtcgcc cgccgcgatg cccgcgagga tcacgacgtt gcccaggcgc gcgtcgcgct
     4261 ctccgagcgt gacgggcacc ttgcggatgg cgtcggcgcc caggcgccag acgtaggcct
     4321 tgtcgccttc cttgacgatc gtcgcctcag gcagcagcag cgcctgcgac gcgcccgagg
     4381 agatgcgccc ctcggcgtac agccccgcca ccttgggcgc cgtgcccggc gcgaagtcga
     4441 cgatcaccgc gacctggcgc gtcaccggat cggcagacgc gtcgacatgg cgcaccttgc
     4501 ccgtgaagtc gcccttgtcg aagccgttga tgcggaactg caccgcctgg ccggcatgga
     4561 tgtcgcccat gcggtcggcc gagaccaggc cctcgaagcg catgctcgcc gggtcgatca
     4621 ccttgagcag ttccttgccg atggccgccg tgtcgccggc cgagaccttg cggtcgctga
     4681 cgacgccgtc gaacggcgcg cgcacctcgg tgcgggcgag ctgctggcgg gcggccaccg
     4741 tgcgcgagtc ggccgcgacg cggtcgctct gcgcgctgtt gcggcgcacc tcggcgtcgt
     4801 cgagcgcctg ctgcgagctc atgccctcgg cctgcagcgt cttgacgcgg gtgaactggc
     4861 gctgcgcctg gtcgagcgcc tgcgcggcgg cgcggctggc ttcctcggcg gagcgcaggc
     4921 tgtcgcggat cgacgtgtcg tccagccgca ccagcaggtc gcccttgcgc accggctcgc
     4981 cgttctcctt gagcacctgc agcacgacgg cggaaacctc ggcgcgcagg tcggcgcggc
     5041 gctcgggctc gaccgagccg gtgaccaccg ggcccgatgc atgcgtctgc aggcccacgg
     5101 tgcgcacgtc ctcgggcgcg atcagcaggg tggccgacgg ggc
//
LOCUS       DATCRO010000676         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_41280, whole genome shotgun sequence.
ACCESSION   DATCRO010000676 DATCRO010000000
VERSION     DATCRO010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41280"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VIN75_18375"
     CDS             <1..1329
                     /locus_tag="VIN75_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976186.1"
                     /translation="AAEAARKKAEQEAAERKRQADEEAARAAKAESQRKAEAAAEAAR
                     LKAEDEARRKADAEARKAAQVEARRVADEQARQKAAEAAQAKAEDEARKKAAAEARRQ
                     AEAEAKAQADAAARERAAFEARRKADEEAARKAEAEAEARRARDAAALRDVDPEATMI
                     GRALPVAADDAETTMVRPIPERPTTPAPAPEPRRPRVDAPAPVPAPAQDKKSMLVPIA
                     GLAVVAVAAGAWFVLRPAPAPKVDYAPPPVASAVAPPVPAPAPAPTPAPAPAPAEQAS
                     APTPSAAPAPAPEAKPPVEDAAAKAKAEAEAKAKAEARAKAKAEAEAAKAQAEAAARI
                     KADADAAAKAQADADARKAKADADAKAKADAEATKPRTDPKADAADTFRRGYEAYLAG
                     HVPEGIRLMERADSMGAPGARSRLCAIYRKPTAAEGKDFLKEANKCKGVD"
     gene            1523..>2664
                     /locus_tag="VIN75_18380"
     CDS             1523..>2664
                     /locus_tag="VIN75_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8976187.1"
                     /translation="MELVVRALRCPPPDQPQAINARFGASGGRIGRSNTCSLPLPSPD
                     RYISREHLEIRCRDGKYSLKVISKVNGCSVNDNAVPPGAAVELSHGDRIQLGDYLLVA
                     ELQNTPTAKDPLAAFGYSSNARADSLDMMASLVPAPPATGGRDSQFDTSPNWAQPVVP
                     AARRAPPAVSPQIETDPSATALGQMLDAAPSASRPRPESPASRAPAFGRDDTGASALD
                     AFLGLDGGLLSKPAAQAPLSDQAQGLYRQQDVNVAMPAQGHNTLPDPFEDDIFSALDK
                     DFGPAARAPAPAPAAPVRPAPPPPAVDPLDIDLMAGMAAPTRGGPAAAPRAAPQAAPA
                     PAAAPGPSVDGAQALAVLAQALELDPGDLDSAHPMETIRIVGELLNL"
BASE COUNT          408 a         1065 c          959 g          232 t
ORIGIN      
        1 gccgccgagg ccgcgcgcaa gaaggcggag caggaagccg ccgagagaaa gcgccaggcc
       61 gacgaggaag cggcgcgcgc cgcgaaggcc gagagccagc gaaaggccga agccgctgcc
      121 gaagccgcgc gcctgaaggc cgaggacgag gcgcgccgca aggccgacgc cgaggccagg
      181 aaggccgcac aggtcgaggc caggcgcgtc gccgacgaac aggcgcggca gaaggccgcc
      241 gaggccgccc aggccaaggc cgaagacgag gcgcgcaaga aggctgctgc cgaggcgcgg
      301 cgccaggccg aggccgaagc caaggcgcag gccgacgcgg ccgcgcggga acgcgccgcc
      361 ttcgaggcgc gccgcaaggc cgacgaggag gccgcccgca aggccgaggc cgaagccgag
      421 gcccgccggg cgcgcgacgc cgcggcgctc agggacgtcg atcccgaggc gacgatgatc
      481 ggccgcgcgc tgccagtcgc cgccgacgac gccgagacga cgatggtgcg cccgatcccg
      541 gagcgcccga ccacccctgc gcccgctccg gagccgcgca ggccgcgcgt cgacgctcct
      601 gcgcccgtgc ccgcgcccgc acaggacaag aagagcatgc tggtgccgat cgccggcctg
      661 gccgtcgtcg cggtggccgc cggcgcctgg ttcgtgctgc gcccggcgcc ggcgcccaag
      721 gtcgactacg cgccgccgcc ggtggccagc gcggtcgcgc cgcccgtgcc ggcacccgct
      781 cccgcgccca cgccggcgcc ggctcccgca ccggccgagc aggcctccgc gccgacgccg
      841 tctgccgcac ccgcacccgc accggaagcc aagccgccgg tcgaggacgc cgcggccaag
      901 gccaaggcgg aagccgaggc caaggccaag gccgaggcgc gtgccaaggc caaggccgag
      961 gcggaggccg ccaaggcgca ggccgaggcc gcggccagga tcaaggcgga cgcggacgcc
     1021 gcggccaagg cgcaggccga cgccgacgcc aggaaggcca aggccgacgc cgacgcgaag
     1081 gccaaggccg acgcggaagc caccaagccc aggaccgacc cgaaggcgga cgccgccgac
     1141 accttccgcc gcggctacga ggcctacctg gccggccacg tgcccgaggg catccgcctg
     1201 atggaacgcg ccgactcgat gggcgccccg ggggcccgct cgcggctgtg cgcgatctac
     1261 cgcaagccga cggccgccga gggcaaggac ttcctcaagg aagccaacaa gtgcaagggc
     1321 gtcgactgac cccggagcgg cccgcggcca ggcgcctcga cgccgcgtga cgagattcgc
     1381 ggcggcgcaa cggcggggga ttaccctcgc accgtcgcgc gccggctgag caaggatgtg
     1441 caagatcccc ccatttcggg tgtcgcccgt gcgcgaggga tccaggcggc gataatcatt
     1501 ttcgtcttct cacgtcattg ccatggaact cgtcgtccgc gcactccgct gccccccgcc
     1561 cgaccagccg caagcgatca acgcgcgctt cggagccagc ggcggacgca tcggccgcag
     1621 caacacgtgc tcgctgccgc tgccgagccc ggatcgctac atctcgcgcg agcacctcga
     1681 gatccgctgc cgcgacggca agtactcgct caaggtcatc tccaaggtca acggctgcag
     1741 cgtcaacgac aacgccgtgc cgccgggcgc cgcggtcgag ctgtcgcacg gcgaccgcat
     1801 ccagctgggc gactacctgc tggtggccga gctgcagaac acgccgaccg ccaaggatcc
     1861 gctggccgcg ttcggctact cctcgaacgc gcgcgccgac agcctcgaca tgatggcctc
     1921 gctggtgccg gcgccgccgg ccacgggggg ccgcgactcg cagttcgaca cgtcgcccaa
     1981 ctgggcccag ccggtcgtgc ccgccgcgcg ccgcgcgccg ccggcggtgt cgccccagat
     2041 cgagaccgac cccagcgcca ccgcgctcgg gcagatgctc gacgccgcgc cgtcggcctc
     2101 gcgcccgcgc cccgagtcgc cggccagtcg cgcgcccgcg ttcggccgcg acgacaccgg
     2161 cgccagcgcg ctcgacgcct tcctcggcct ggacggcggc ctgctgtcca agcccgccgc
     2221 gcaggccccg ctcagcgacc aggcgcaggg cctgtaccgc cagcaggacg tcaacgtggc
     2281 gatgcccgcg cagggccaca acacgctgcc cgacccgttc gaggacgaca tcttctcggc
     2341 gctggacaag gacttcggcc ccgccgcgcg cgcaccggcg cccgcgcccg ccgcgccggt
     2401 gcgcccggcc ccgccgccgc ccgccgtcga tccgctggac atcgatctca tggccggcat
     2461 ggccgcgccc acgcgcggcg ggcctgccgc ggcgccgcgc gccgcgccgc aggcggcgcc
     2521 tgcgccggcg gccgcaccgg gcccgtcggt cgatggcgcc caggcgctgg ccgtgctcgc
     2581 gcaggcgctc gaactcgatc cgggcgatct ggactcggcg catccgatgg agacgatccg
     2641 catcgtcggc gagctgctga acct
//
LOCUS       DATCRO010000677         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_401495, whole genome shotgun sequence.
ACCESSION   DATCRO010000677 DATCRO010000000
VERSION     DATCRO010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401495"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..337)
                     /locus_tag="VIN75_18385"
     CDS             complement(65..337)
                     /locus_tag="VIN75_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976188.1"
                     /translation="MKAVADILQRAVSGASVRPDGRGYTVPRSWGVYRLPADAGATRR
                     LRFGNHPVRQVELEREFGSCRLEYLFLARADAAAVAAALNEDGRQR"
     gene            462..764
                     /locus_tag="VIN75_18390"
     CDS             462..764
                     /locus_tag="VIN75_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8976189.1"
                     /translation="MILEHADITIAPGRNAEFEEAIRHGIATVISTAKGFVDAQVHKG
                     IESPERYVLTIHWQTLENHTVDFRGGPLFPAWRAIVGPFFAKPPVVEHFELVASPA"
     gene            complement(779..1957)
                     /locus_tag="VIN75_18395"
     CDS             complement(779..1957)
                     /locus_tag="VIN75_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8976190.1"
                     /translation="MPDHALLQIQPLGFPWETADPFLFCVHHDDAYPKGNGRFGPDAS
                     LAGRAIGQDFGGKDGWNMYHGQTVPGFPSHPHRGFETVTIVRQGLTDHSDSLGAAARF
                     GGGDVQWLTAGKGIVHSEMFPLLETDRPNPLELFQIWLNLPARSKMCEPHFTMFWAGD
                     IPHRVFTSEGGATTEVAVIAGRLEGPPSSGGAADVAQALPPPPDSWAAQAEADVAIWT
                     LRLQPGARWTLPAAANAAARRKLYFFAGSGVSVAGQAIAEHAAIELRAASEVELVNTG
                     ADLAEFLLLQGRPIGEPVAQYGPFVMNTEAELRQAFDDYRRTEFGGWPWPDPGPVHGP
                     ARQRFARHADGRVETLDAARENRAVMANVVPTDVAEPQDARPVTSSEALMENASRGMQ
                     "
     gene            complement(2048..>2302)
                     /locus_tag="VIN75_18400"
     CDS             complement(2048..>2302)
                     /locus_tag="VIN75_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEY8976191.1"
                     /translation="VVLEGQEAAGKGDNNFDADVAIATGELGKLIPDLVEALGGPLKL
                     GQAGTSTTDKVIAALTGQTAAMDTASPYAPANAEDAAAPF"
BASE COUNT          315 a          829 c          837 g          321 t
ORIGIN      
        1 tgccagtacc ccacaggcac gatgacggac gcggcgccca cgagccacga aacgcgccgc
       61 agcctcatcg ttggcgcccg tcctcgttga gcgccgcggc cacggccgcg gcgtccgcgc
      121 gcgccaggaa caggtactcc agcctgcacg agccgaattc gcgttcgagc tcgacctggc
      181 gcaccgggtg gttgccgaag cgcagccgcc gcgtcgcgcc ggcgtccgcg ggcagccgat
      241 agacccccca gctgcgcggc acggtgtacc cgcggccatc gggacggacg ctggcgccgc
      301 tgacggcgcg ctggaggatg tcggcaacgg ctttcatcga cggggtctcc gatgtaggcg
      361 ccgatcatag gatgcggcac cggcgccggg aaggcgtgcc ctccgcgcgg gaaatcgcga
      421 acggtcagaa tcgccttctc aactgccgcg agcacgacga catgatcctc gaacacgccg
      481 acatcaccat cgcccccggc aggaacgccg aattcgagga agcgatccgg catggcatcg
      541 ccaccgtcat ctccaccgcc aagggcttcg tcgacgcgca ggtgcacaag ggcatcgagt
      601 cgcccgagcg ctacgtcctg acgatccact ggcagacgct ggagaaccac accgtcgatt
      661 tccgcggcgg cccgctgttc cctgcctggc gcgcgatcgt cggcccgttc ttcgcgaagc
      721 cgccggtggt ggagcatttc gagctggtgg cctcgccggc ctgagaccgg cagcgccgtc
      781 actgcatccc gcgcgacgcg ttctccatca acgcctcgct cgacgtgacc ggccgcgcgt
      841 cctgcggctc cgcgacatcg gtcgggacga cgttggccat caccgcgcgg ttctcgcgcg
      901 cggcgtccag cgtctcgacg cggccgtcgg cgtggcgcgc gaagcgctgg cgggcggggc
      961 cgtgtaccgg gcccgggtcg ggccagggcc agccgccgaa ttcggtgcgg cggtagtcgt
     1021 cgaacgcctg gcgcagctcg gcctcggtgt tcatcacgaa cgggccgtac tgggccaccg
     1081 gctcgccgat ggggcggccc tgcaggagca ggaactcggc caggtccgcg cccgtgttga
     1141 ccagctcgac ctcgctggcc gcgcgcagct cgatcgcggc gtgctccgcg atggcttggc
     1201 cggcgacgct cacgccgctg cccgcgaaga agtacagctt gcggcgcgcc gcggcgttgg
     1261 cggccgcggg cagcgtccag cgcgcgccgg gctgcagccg cagcgtccag atcgcgacgt
     1321 cggcctcggc ctgcgcggcc cacgagtcgg gcggcggcgg cagcgcctgc gcgacgtccg
     1381 cggcgccccc gctggagggc ggcccctcga ggcggcccgc gatgacggcc acctcggtgg
     1441 tcgcgccacc ctcgctcgtg aacacgcgat gcgggatgtc gccggcccag aacatcgtga
     1501 aatgcggctc gcacatcttg ctgcgcgcgg gcaggttcag ccagatctgg aacagctcca
     1561 gcgggttggg ccggtccgtc tcgagcagcg ggaacatctc cgagtggacg atgcccttgc
     1621 cggccgtgag ccactgcacg tcgccgccgc cgaagcgcgc ggcggcgccc agcgagtcgg
     1681 aatggtcggt caggccctgg cggacgatgg tgaccgtctc gaagccgcga tgcgggtgcg
     1741 acgggaagcc aggcaccgtc tggccgtggt acatgttcca gccgtccttg ccgccgaagt
     1801 cctgcccgat cgcgcggccg gccagcgacg cgtccgggcc gaagcggccg ttgcccttgg
     1861 ggtacgcgtc gtcgtgatgg acgcagaaca ggaacgggtc cgcggtctcc cacgggaagc
     1921 cgagcggctg gatctgcagg agggcgtggt cgggcatggc gggttccgtc gagcgggcag
     1981 gcgatgggag gagtctagcc agacgccgcc atcgccccgc tcgaaggggc atccgcgatg
     2041 cctgtcctca gaacggcgcc gcggcgtcct ccgcgttggc cggcgcgtac ggcgaggccg
     2101 tgtccatcgc ggcggtctgg ccggtcagcg cggcgatcac cttgtcggtg gtggacgtgc
     2161 cggcctgccc gagcttgagc gggccgccca gcgcctcgac caggtcgggg atgagcttgc
     2221 cgagctcgcc ggtggcgatc gcgacgtcgg cgtcgaagtt gttgtcgccc ttgcccgcgg
     2281 cctcctggcc ctcgagcacg ac
//
LOCUS       DATCRO010000678         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_322898, whole genome shotgun sequence.
ACCESSION   DATCRO010000678 DATCRO010000000
VERSION     DATCRO010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322898"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VIN75_18405"
     CDS             <1..277
                     /locus_tag="VIN75_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8976192.1"
                     /translation="IHRQHLDAADGSADERMAAFLVDLHERRRALGDTGARLPLPMSR
                     ADIGNHLRLATETVSRLLARFRDAGLLRVTRSGVELLHLDRLRRVAT"
     gene            complement(345..>2148)
                     /locus_tag="VIN75_18410"
     CDS             complement(345..>2148)
                     /locus_tag="VIN75_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY8976193.1"
                     /translation="GWRSLRPWSPNMYTLIALGTGVAWLYSVVALLAPGLFPAGFRMH
                     GRVGVYFESAAVIVVLVTLGDWLELRARRRTGEALKALLGLAPKTAHRLEHGHERDVP
                     LDAVRVGDLLRVRPGEKVPVDGEAIEGESHVDESMLTGEAMPIAKAAGDRLAAGTVNQ
                     DGMLLLRAGKVGGETMLAQIVALVAQAQRSRAPLQRVADRVAAWFVPAVVAVAVLASA
                     AWAAFGPEPRLAHALIALVSVLIIACPCALGLATPISIMVASGRGAHEGVLFKDAAAI
                     ERLRDVDTLVLDKTGTLTEGKPALQDVVSTGALPWDQLLAVAAALERPSEHPLARAIL
                     AGAEARGVKVPEVDAFRTLTGGGVQGRVAGRDAALGNARLFESLRIAIGDEARQRADA
                     MRKDGATVMFLAVDGALAGLLAVADRLKPSTPPAIAALHAAGLRIVMLTGDNAVTAQA
                     VAKTLGIDAVHADVSPADKAAVVNALRAEGRRVAMAGDGINDAPALAAADIGIAMGGG
                     TDVAIESAQLTLVKGELTAILRARALSQATVANIRQNLFFAFVYNALGVPLAAGVLYP
                     WLGLTLSPMVAALAMSLSSVSVVGNALRLSRQRA"
BASE COUNT          308 a          830 c          730 g          280 t
ORIGIN      
        1 gatccatcgc cagcacctgg acgccgccga cggttcggcc gacgagcgca tggcggcttt
       61 cctggtcgac ctgcacgaac gccgcagggc actgggcgac accggcgccc ggctgccgct
      121 gccgatgtcg cgcgccgaca tcggcaacca tctgcgcctg gccaccgaaa cggtcagccg
      181 cctgctggcg cggttccgcg atgcggggct gctgcgggtg acgcgcagcg gcgtggaact
      241 gctgcatctg gatagactgc gccgcgtggc cacgtaggtt ggggtgagcc acaggcgaac
      301 cccaacgatg ccgcacgctt gaaggcagcg atgttgggcg cccgttacgc gcgctgccgg
      361 ctcaggcgca gggcattgcc taccaccgac accgacgaca ggctcatcgc cagcgccgcc
      421 accatcggcg acagggtcag gcccagccac ggatacagca cgcccgccgc cagcggcacg
      481 cccagcgcgt tgtagacgaa ggcgaagaac aggttctggc ggatattcgc caccgtcgcc
      541 tgcgacagcg cacgcgcgcg caggatcgcg gtcagctcgc ccttcaccag ggtcagctgc
      601 gcgctctcga tcgccacgtc ggtgccgccg cccatggcga tgccgatatc cgccgcggcc
      661 agggcgggcg cgtcgttgat gccgtcgccg gccatcgcca cgcgccttcc ttcggcacgc
      721 agcgcgttga ccaccgcggc cttgtcggcc ggcgagacat cggcgtgcac cgcgtcgatg
      781 cccagcgtct tcgccaccgc ctgcgcggtg acggcgttgt cgccggtaag catcacgatg
      841 cgcaggccgg ccgcatgcag cgccgcgatc gccggcggcg tgctcggctt caaccggtcg
      901 gccaccgcca gcaggccggc gagcgcgccg tccacggcga ggaacatcac cgtcgcgccg
      961 tccttgcgca tggcgtcggc gcgctgccgc gcttcgtcgc cgatggcgat cctcaagctt
     1021 tcgaacaggc gcgcattgcc cagcgccgcg tcgcgcccgg ccaccctgcc ctgcacgccg
     1081 ccgccggtaa gcgtgcggaa cgcgtccacc tccggcacct tgacgccgcg cgcctcggcg
     1141 ccggccagga tcgcgcgcgc cagcggatgc tcgctggggc gttccagcgc ggcggccacg
     1201 gccagcagct gatcccacgg cagcgcgccg gtgctcacca catcctgcag cgcaggcttg
     1261 ccctcggtga gcgtgccggt cttgtccagc accagggtgt cgacgtcgcg cagcctttcg
     1321 atcgccgccg cgtccttgaa cagcacgcct tcgtgcgcgc cgcgcccgct ggccaccatg
     1381 atcgagatcg gcgtggccag gcccagcgcg cacggacagg caatgatcag caccgacacc
     1441 agcgcgatca gcgcatgcgc cagccgcggc tccggtccga acgcggccca ggcggcggag
     1501 gccagcaccg ccaccgcgac caccgcgggg acgaaccagg ccgccacacg gtcggccacg
     1561 cgctgcagcg gtgcgcggct gcgctgggcc tgtgcgacca gcgcgacgat ctgcgccagc
     1621 atcgtctccc cgcccacctt cccggcgcgc agtagcagca tgccgtcctg gttgaccgtg
     1681 cccgcggcga gacggtcgcc ggccgccttg gcgatgggca tggcctcgcc ggtgagcatg
     1741 gactcgtcga cgtggctttc cccttcgatg gcctcgccgt cgaccggcac cttctcgccc
     1801 ggacgcacgc gcaacaggtc gcccacgcgc accgcgtcca acgggacgtc gcgttcgtgg
     1861 ccgtgttcca ggcgatgcgc ggtcttcggc gccaggccca acagcgcctt gagcgcctcg
     1921 cccgtgcgtc ggcgtgcgcg cagttccagc cagtcgccca gcgtgaccag cacgacgatc
     1981 accgcggccg actcgaagta cacgcccacg cgcccgtgca tgcggaaccc ggccgggaac
     2041 aggccgggcg cgagcagggc gaccacgctg tagagccagg ccacgccggt gcccagtgcg
     2101 atcagcgtgt acatgttcgg cgaccacggg cggagcgagc gccagccg
//
LOCUS       DATCRO010000679         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_694070, whole genome shotgun sequence.
ACCESSION   DATCRO010000679 DATCRO010000000
VERSION     DATCRO010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694070"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..563
                     /locus_tag="VIN75_18415"
     CDS             123..563
                     /locus_tag="VIN75_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY8976194.1"
                     /translation="MASPSNILYTGTATATGGRAGTARSSDGALSVTLTTPKQLGGDG
                     AAGTNPEQMFAAGYSACFIGAMKAVASGMTGQPKVALPADVSITSDVGIGPIPGGFGI
                     KVDMRISIPGMDKAAAEALVAAAHQVCPYSNATRGNIDVSLTVV"
     gene            695..1546
                     /locus_tag="VIN75_18420"
     CDS             695..1546
                     /locus_tag="VIN75_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976195.1"
                     /translation="MLLAATVAAALVGGALVYGLDHGQPQAAAHPAPAVEPAHDEPPA
                     VMSSPTPVARAMPPARPASAARPDPFESLSQKVDRLGRSADPVDAFAAYQLVTACVCA
                     RDHEGWMAHHILPSDRAMLPTPQQACGDIASDQIQSRLRWLERAALAGVHHAATEMLR
                     EGPDGLGMSNSDIEAPENASWKLRVEAALDAGVHTCDPDSLESRVNAYENGQGVAPDR
                     ARALTYWVAYMECRQRLVGQPSSILANADSVTQQMGRSLSADQIAQAVAAGHALARDA
                     RPLPGDQ"
     gene            complement(1553..2497)
                     /locus_tag="VIN75_18425"
     CDS             complement(1553..2497)
                     /locus_tag="VIN75_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8976196.1"
                     /translation="MQSPKPLVVILATGGTIAGTAALATDTTGYRAGALGIDALVAGV
                     PALADHRLESESVAAIDSKDMDEATWQRLARRVAHHLARPEVAGIVITHGTDTLEETA
                     YFLHRVLAPAKPVVLTAAMRPASSLSADGPANLLDAVTVAESPGVRGVSVVFGGSVWG
                     AVGLRKVHTLQTSAFTGSDCGPIARLEGGAMRVFRAWPEGEALGLARIAAEPSTWPRV
                     EIVLNHAGADGRIVDDAVIAGTAGIVVAGTGHGTVSARMDAAIARARGEGVRVTRASR
                     CVFGPVLPHGAEDADAAGELSAVQARVELQLALLADAR"
     gene            2587..3267
                     /gene="lexA"
                     /locus_tag="VIN75_18430"
     CDS             2587..3267
                     /gene="lexA"
                     /locus_tag="VIN75_18430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8976197.1"
                     /translation="MEVESPKLTARQQQILDLVQSAIERTGAPPTRAEIANELGFRSA
                     NAAEEHLQALARKGVIELVGGTSRGIRLKSDTLRAVNEARGRQFSLPLPSLSQLTLPL
                     VGRVAAGSPILAQEHVEQTYLLEATMFPRRPDYLLKVKGMSMRDAGIIDGDLLAVQKC
                     REARNGQIVVARLGDEVTVKRFRRTRTTIELLPENPDFQPIIVPFGDVDFELEGIAVG
                     LVRNNMMS"
     gene            3379..3678
                     /locus_tag="VIN75_18435"
     CDS             3379..3678
                     /locus_tag="VIN75_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8976198.1"
                     /translation="MKSPKAATADECLALEQLPNVGPAMAADLRLLGIHAPQALKGRD
                     GLQLYRALCTATGQRHDPCVLDTLLAIVDFMDGAPPAPWWAYTPRRKAMVGQLRD"
BASE COUNT          544 a         1497 c         1314 g          472 t
ORIGIN      
        1 gacctgaccc gccagctcac cgccatgcgc gatgcgctga cccgccaggc tgagcactga
       61 ctcatttcct ttcgcgcgaa tccccgcgcc cccacgaccc cgaccaaccc aaggagcaca
      121 ccatggcctc tccctcgaac attctgtaca ccggcaccgc caccgccacc ggcggccgcg
      181 ccggcaccgc caggtcctcg gacggcgcgc tgagcgtcac gctgaccacg cccaagcagc
      241 tgggcggcga cggcgccgcc ggcaccaacc ccgagcagat gttcgcggcc ggctactcgg
      301 cctgcttcat cggcgcgatg aaggccgtgg cctcgggcat gaccggccag cccaaggtcg
      361 ccctgcccgc cgacgtgtcg atcacctccg acgtcggcat cggcccgatc cccggcggct
      421 tcggcatcaa ggtcgacatg cgcatctcga tccccggcat ggacaaggcc gccgccgagg
      481 cgctggtcgc cgccgcgcac caggtctgcc cgtactcgaa cgccacccgc ggcaacatcg
      541 acgtctcgct gacggtggtc tgacgtcccg caccgcggcc tgcacgcgat gacgcgggcc
      601 gcgcaggatg tcaccggaca ggcccgccgc gcgcgggcct gcgccatggc ggcgccgggg
      661 cgtcccctat gatgccgcgg tgcaagaccg gcccatgctc ctcgcggcga ccgtcgccgc
      721 cgccctggtc ggcggcgcgc tcgtctacgg cctggaccat ggccagccgc aggccgcggc
      781 gcacccggcg cctgcggtcg aaccggcaca tgacgagccg ccggcggtga tgtcgtcgcc
      841 gacgccggtc gcgcgcgcca tgccacccgc caggcccgcg tccgccgcgc ggcccgaccc
      901 cttcgaatcg ctgtcgcaga aggtcgaccg gctcggccgc tcggccgacc cggtcgacgc
      961 gttcgcggcc taccagctcg tcaccgcctg cgtctgtgcg cgcgaccatg agggctggat
     1021 ggcgcaccac atcctgccga gcgaccgggc gatgctgccc acgccgcagc aggcctgcgg
     1081 cgacatcgcc tccgaccaga tccagagccg cctgcgctgg ctcgagcgcg ccgcgctcgc
     1141 gggcgtgcac cacgccgcca ccgagatgct gcgcgaaggc ccggacggcc tgggcatgag
     1201 caactccgac atcgaagcgc ccgagaacgc gtcgtggaag ctgcgcgtcg aggccgcgct
     1261 cgacgcgggc gtgcacacct gcgaccccga ctcgctcgag agccgcgtca acgcctacga
     1321 gaacggccag ggcgtcgcgc ccgaccgcgc cagggcgctc acgtactggg tcgcgtacat
     1381 ggaatgccgc cagcgcctgg tcggccagcc gagctcgatc ctcgccaacg ccgacagcgt
     1441 cacgcagcag atgggccgct cgctgtcggc cgaccagatc gcccaggccg tggccgccgg
     1501 ccacgcgctg gcgcgcgacg cgcgcccgct gcccggcgac cagtgagccg cgtcagcgcg
     1561 cgtcggcaag aagggccagc tgcagctcga cccgcgcctg cacggcgctg agttcgccgg
     1621 cggcgtcggc gtcttccgcg ccgtgcggca gcaccggccc gaacacgcag cggctcgcgc
     1681 gcgtcacgcg cacgccctcg ccgcgcgcgc gggcgatcgc cgcgtccatg cgcgccgaca
     1741 cggtgccgtg gcccgtgccc gcgaccacga tcccggccgt gccggcgatg acggcgtcgt
     1801 cgacgatgcg gccgtccgcg ccggcgtggt tcagcacgat ctccacgcgc ggccacgtcg
     1861 agggctccgc cgcgatgcgg gccaggccca gtgcctcgcc ctcgggccac gcgcggaaca
     1921 cgcgcatcgc gccgccctcg agccgcgcga tcgggccgca gtcgctgccg gtgaacgcgc
     1981 tggtctgcag cgtgtgcacc ttgcgcaggc cgaccgcgcc ccacaccgag ccgccgaaga
     2041 cgacgctgac cccgcgcacg ccgggcgact cggccacggt gacggcgtcc agcaggttgg
     2101 ccgggccgtc ggccgacagc gagctggccg gacgcatcgc cgcggtcagc acgaccggct
     2161 tggccggcgc gagcacgcgg tgcaggaagt acgcggtctc ctccagcgtg tcggtgccgt
     2221 gcgtgatgac gatgcccgcg acctcgggcc gcgccaggtg gtgcgccacc cgccgcgcga
     2281 ggcgctgcca cgtggcctcg tccatgtcct tgctgtcgat ggcggcgacc gattcggact
     2341 cgagacggtg gtcggccagc gccggcacgc ccgccacgag ggcgtcgatg cccaacgctc
     2401 cggcgcggta gccggtggtg tcggtggcga gggcggcggt gccggcgatc gtcccgcccg
     2461 tggcgaggat cacgacgagc ggcttggggc tttgcattct tggcaacctt ggataaaaat
     2521 acagacctgt atgaacaacc agatgccggc cgcgggacgc gagtctcctg acggacagga
     2581 cgcaccatgg aagtcgaatc gccgaagctc accgcccgcc agcagcagat cctcgatctc
     2641 gtgcagagcg cgatcgaacg caccggcgcg ccgccgaccc gggccgagat cgccaacgaa
     2701 ctcggcttcc gctcggccaa cgcggcggag gaacacctcc aggccctggc ccgcaagggt
     2761 gtcatcgagc tggtgggcgg cacctcgcgc ggcatccggc tgaagtctga cacattgcgc
     2821 gccgtcaacg aggcgcgcgg gcgccagttc tcgctgccgc tgcccagcct gtcgcagctc
     2881 acgctgccgc tggtgggacg cgtggcggcg ggctcgccca tcctcgcgca ggagcacgtc
     2941 gagcagacct acctgctcga ggccacgatg ttcccgcgcc ggcccgacta cctgctcaag
     3001 gtcaagggca tgagcatgcg cgacgccggc atcatcgacg gcgacctgct ggccgtgcag
     3061 aagtgccgcg aggcgcgcaa cggccagatc gtggtcgcgc gcctgggcga cgaggtcacc
     3121 gtcaagcgct tccgccgcac ccgcaccacg atcgagctgc tgccggagaa tcccgacttc
     3181 cagcccatca tcgtgccgtt cggcgacgtc gacttcgagc tcgagggcat cgccgtcggc
     3241 ctcgtgcgca acaacatgat gagctgagca tgcctgccgg ctcgcccagt cccaccccgc
     3301 gccgcgcgtg cgtcacgcgc gcgcggcgct agtggcggcg ccgtgggcag ctcgtcgcgc
     3361 tcgcggggcc gcacggccat gaagtcgccc aaggcggcga cggccgacga gtgcctcgcg
     3421 ctcgagcagc tgcccaacgt cggcccggcc atggccgccg acctgcgtct gctgggcatc
     3481 cacgcgccgc aggcgctcaa gggacgcgac ggcctgcagc tgtaccgcgc cctgtgcacg
     3541 gccaccggcc agcgccacga cccgtgcgtg ctggacacgc tgctggccat cgtcgacttc
     3601 atggacggcg ccccgccggc gccgtggtgg gcctacacgc cgcgacgcaa ggcgatggtc
     3661 gggcagttgc gcgactgacg cacggcgcgg gccgcgcagc cgcggtcgcc aaatcgagca
     3721 gccatcaggc ctggtagtgc tcgtcccagc tgccggccac cgcccatccg aagtggcgga
     3781 tcccgcgctc gagcgcgctg ccgagcttgg cgatggtctc gatcggc
//
LOCUS       DATCRO010000680         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_446133, whole genome shotgun sequence.
ACCESSION   DATCRO010000680 DATCRO010000000
VERSION     DATCRO010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446133"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VIN75_18440"
     CDS             complement(<1..575)
                     /locus_tag="VIN75_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8976199.1"
                     /translation="MKKANETSEGDGAAVVDAPESKPAKAPRKPRGRPLSFDRDAALE
                     TAMHVFWERGYEAASISDLTSAMGITPPSLYTAFGDKEQLFLEAIERYALGYGSAGVR
                     ALKEEPTARGAIERWLMEAANELTQPCHPKGCMVVMAATNCSAAAERVQDALLLRRTE
                     AIANVGRRIQGGIDNGELPPDTDAQDLANFYA"
     gene            768..1976
                     /locus_tag="VIN75_18445"
     CDS             768..1976
                     /locus_tag="VIN75_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976200.1"
                     /translation="MNQVPLQARRRLWVAGTTVTAVVAALASVFMLESGPAKATPSPA
                     AQAMPVSVAKVEQSDVATWNEFSGRLEAVERVDIRSRVAGTVQAVHFREGALVKAGDL
                     LLTIDPAPYQADVDRAEAQVLAAQARLTNARTNYARSKQLLEDHAISQREYDDALNAQ
                     SEADANLRAAQAQAQSARLSLGYTQVRAPVSGRVGKLEITTGNLVAAGPGAPVLTTLV
                     SVSPIYASFDADEQVVARALRELPAGASARAQIEKIPVQMGTVADTATPIAGHLQLVD
                     NQVDARSGTVRVRAVFDNADGSLMPGQFAHLRMGQVKADPALLINERAVGTDQSKKFV
                     MVVGADDKAQYREVTLGANVDGLRIVTSGLKPGERIVVNGLQRVRPGALVAPQVVAMR
                     SPDDAAAAKN"
BASE COUNT          299 a          714 c          698 g          299 t
ORIGIN      
        1 gcgtagaagt tcgccaggtc ctgcgcgtcc gtgtcgggtg gcagctcgcc gttgtcgatg
       61 ccgccctgga tgcggcggcc gacgttggcg atggcctccg tgcggcgcag cagcagcgcg
      121 tcctgcacgc gctcggcggc ggcggagcag ttggtggcgg ccatcaccac catgcagcct
      181 ttcgggtggc agggctgcgt gagctcgttg gcggcttcca tcagccagcg ctcgatcgcg
      241 ccgcgcgccg tcggctcttc cttcagcgcg cgcacgccgg cgctgccgta gcccagcgcg
      301 tagcgctcga tggcttcgag gaacagctgc tccttgtcgc cgaaggcggt gtacaggctg
      361 ggcggggtga tgcccatcgc ggacgtcagg tcggagatgg acgcggcctc gtagccgcgt
      421 tcccagaaca cgtgcatcgc ggtctctagc gcggcgtcgc ggtcgaacga cagcgggcgg
      481 ccgcgcggct tgcgcggggc cttggccggc ttcgattcag gggcgtcgac gacggcggcg
      541 ccgtcgcctt ccgatgtctc attcgccttt ttcatagcgg tcgctaataa aatctgttga
      601 cgtgcccttg tggcttggct acattgtgcc atatgtcgta gcgatcgcta cgataaaagt
      661 tcagcaatca gcgaaacgac agcccggcga gcccgggttc agccgctgca ttgccagaag
      721 tttttgagca cttacttttc agattcgcct tgtcttcagg agttcccatg aaccaggttc
      781 cgctccaagc ccgccgccgc ctctgggtcg ccggcaccac cgtgacggcg gtcgtcgccg
      841 ccctcgccag cgtcttcatg ctggaatccg gccccgccaa ggccaccccg tcgccggccg
      901 cccaggccat gccggtgtcg gtcgccaagg tcgagcagag cgacgtggcg acctggaacg
      961 agttctccgg ccgcctcgag gccgtcgagc gcgtggacat ccgctcgcgc gtggccggca
     1021 ccgtccaggc ggtgcacttc cgcgagggcg cgctggtcaa ggccggcgac ctgctgctga
     1081 cgatcgaccc ggcgccctac caggccgacg tcgaccgcgc cgaggcccag gtgctcgccg
     1141 cgcaggcgcg cctgaccaac gccaggacca actacgcgcg ctccaagcaa ctgctggaag
     1201 accacgcgat ctcgcagcgc gagtacgacg acgcgctcaa cgcgcagagc gaggccgacg
     1261 ccaacctgcg cgccgcgcag gcgcaggcgc agtccgcccg gctgagcctg ggctacacgc
     1321 aggtgcgcgc gccggtgtcg ggccgcgtcg gcaagctgga gatcaccacc ggcaacctcg
     1381 tggccgccgg tccgggcgcg ccggtgttga cgacgctggt ctcggtcagc ccgatctacg
     1441 cgagcttcga cgccgacgag caggtcgtcg cgcgcgcgct gcgcgagctg ccggccggcg
     1501 cgagcgcccg cgcgcagatc gagaagatcc cggtgcagat gggcacggtc gccgacacgg
     1561 ccacccccat cgccggccac ctgcagctgg tcgacaacca ggtcgacgcg cgcagcggca
     1621 cggtgcgcgt gcgcgccgtg ttcgacaacg ccgacggcag cctgatgccc ggccagttcg
     1681 cgcacctgcg catgggccag gtcaaggccg acccggcgct gctgatcaac gagcgcgcgg
     1741 tgggcacgga ccagagcaag aagttcgtga tggtggtggg cgccgacgac aaggcgcagt
     1801 accgcgaggt cacgctcggc gccaacgtcg acggcctgcg catcgtcact tccgggctga
     1861 agccgggcga gcgcatcgtg gtcaacggcc tgcagcgcgt gcgccccggc gcgctggtcg
     1921 cgccgcaggt ggtcgcgatg cggtcgcctg acgacgccgc cgccgcgaag aactgatccc
     1981 cagaacaggc ccaagaagcc ctccgcagcc
//
LOCUS       DATCRO010000681         9636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_660302, whole genome shotgun sequence.
ACCESSION   DATCRO010000681 DATCRO010000000
VERSION     DATCRO010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660302"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VIN75_18450"
     CDS             <1..1521
                     /locus_tag="VIN75_18450"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976201.1"
                     /translation="DELFMQAPAGIAVLDGPEHVFELANPAYHAFIGGRDIVGKRLVD
                     ALPEMRGQPFPALLDEVYRTGIAHVGNEAPVQLLRRPDGAVEEAFFNFVYQPLRDGRG
                     LTSGILVFAYEVTEQVNARRHVEHLADELRAEHRRKDDFLAMLAHELRNPLAPVSAAA
                     EVLRLGHANDPRLRRTSEIVTRQVRHMATLIDDLLDASRVTRGLVSIASSCEDLRDII
                     AAAIEQAAPLMDVRRHRLTVSTGSEFVCVLGDRKRLVQAVANLLDNAAKYTAEGGSIE
                     LTLDHDDREATLHVRDNGMGVPPEMRERIFDLFVQGQRGADRAQGGLGIGLALARRLV
                     ELHGGSLACIDTPSGHGSDFRIRLPLAPASAPCASRARAAAREEAVGAARPTILIVDD
                     NDDAARMLAMLLEDSGYEVMTENDPVAALERAARTRPDVFLLDIGLPRMDGHELARRL
                     RATDAGRDATLVALTGYGQATEREKAAQAGFDHYMVKPPDPVALRQLLGSVVPASA"
     gene            complement(1532..1912)
                     /locus_tag="VIN75_18455"
     CDS             complement(1532..1912)
                     /locus_tag="VIN75_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976202.1"
                     /translation="MPTRSCIVVADDNRDAADSLAQLLGLMGYDAVAVYDGEQAVRAC
                     HDLHPDLAILDVQMPVLDGCSAARRIRDDDDVRPPMLASLSALKMQDEPLRSEHELFD
                     THLSKPLRMNELSTLLARTTTFAR"
     gene            complement(1987..2772)
                     /locus_tag="VIN75_18460"
     CDS             complement(1987..2772)
                     /locus_tag="VIN75_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY8976203.1"
                     /translation="MTTTLTAPHRPFVQVDVPRWRLWPVLLAGVLMQGVLWPARELAR
                     LIAHQAAPFFNAHVWAFVGLAMVFQTLAGLGCIAVMRRVLPQAPTYLAWPARGRSLVG
                     LALVIGVGMGVLMLVADWWPQLAAHRAPEAGYDPSHGGVAGWLVVMACSGLCEEPIFR
                     GLLVGLLTALVPGRVRVGRVDLPVSGVLVALLFGLAHYESFRVDPLSLAIAQQLYAFA
                     FGLIYVWLMERSRSLLAPVVAHGVGDAVEVGAVMWLQANWAAH"
     gene            3019..3378
                     /locus_tag="VIN75_18465"
     CDS             3019..3378
                     /locus_tag="VIN75_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976204.1"
                     /translation="MAKILFVDDSRDSADSLATFFKLLGHETEVAYDGDAAVELSTAF
                     RPDIAFIDLQMPMLDGFDTAREFRSRLGERPVLIALTGQEWERTGDAADRAGFDGYLQ
                     KPAQASQLAKLVDNLAS"
     gene            complement(3471..4082)
                     /locus_tag="VIN75_18470"
     CDS             complement(3471..4082)
                     /locus_tag="VIN75_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976205.1"
                     /translation="MANSRAPAGPPSYSTAHLARRLGVSVPTVQRWVDLGHLKAWKTV
                     GGHRRIDADSAEEFIRAQMQQRGGAATGAAAEVVVPFTVLVVDDSADDRDLLQALVEI
                     AVPGARITCADNGFDALIEVGKAMPDLIVTDISMPYIDGVQMIRHLSAPGHARPPAIV
                     AVSSHGPAHVEKLGGLPPGVALLAKPVEPARFVETVNAALDGA"
     gene            complement(4138..6996)
                     /locus_tag="VIN75_18475"
     CDS             complement(4138..6996)
                     /locus_tag="VIN75_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976206.1"
                     /translation="MHPGRLSLRTKLLAFNVGMVVVTLAVLLGLDLAFERVRINGPLY
                     KAIDGGQAVVADILPPPLFIVESHLTVFELVDAQAHGDTARVGLLEALLHERESEFRT
                     RRAEWARTLPPGAVKDSLVDAACAPAATYFQVVERDLLPALHRNDIVTARELLAHTLQ
                     PLFADHRDRIEFAVAAAQRENAQREASAATFVERVRIMMLACWIALALAAFVIVRRVL
                     MKPIVERVAEVGGVLERIGAGDTRTPVVAAGGDEFDQILVAIDRMRARLASAVDTVEG
                     QRHDLESALVEARSATRAKSEFLANMSHEIRTPMNAILGMTDLALRTELTDKQRGYLG
                     QARAAAEALLLVIDDILDFSKIEAGRLVLESRDFSLDEVFDRVTALIGLKAQRKGLEL
                     LLKTSPEVPPVLVGDPMRLLQVLVNLCSNAVKFTDKGEIVVVTVRSVASQADSVLLRF
                     DVKDTGIGLAPAQIEDLFRPFSQVDASSTRVHGGAGLGLAICRKLVGLMDGEIGVESA
                     PGLGSDFHFTARFRVGNPTQAARLPQAGAHRDLRILVVDDSANSREILNDLLVLLGYA
                     PVVEASGAAGLAELRRAAGVGRPYQLVLLDWRMPGLDGFDVMRMLREDPLPGPAPAIL
                     LVTAYGDEAIVREAARQGVAGCLAKPVGASTLLDAINGACGPQRGGPAPAPAAAPARG
                     AAPRARAPEALRGRRVLLVEDNELNRFVATELLCDVAGMDVTLAGNGHEALAQLREAR
                     FDLVLMDIQMPGMDGLQCTRLIRADQAFAKLPVIAMTAHAMAGDRDKSTAAGMNDHVN
                     KPFEPAQLFEVMLRWLTPAGPVGVAAPVAERPPASGPVVDFELGLRRCLGRPELYHKI
                     LARYLKQRETSGNEIRAALDAGELEVGARIAHTLVSTASMLGAPALAALARELHDAVD
                     LGLPERWRALLGRLEAVQAEIDVEVARHMQDVAPPERQ"
     gene            complement(7063..7986)
                     /gene="panE"
                     /locus_tag="VIN75_18480"
     CDS             complement(7063..7986)
                     /gene="panE"
                     /locus_tag="VIN75_18480"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8976207.1"
                     /translation="MRILIVGAGSTGGYFGARLVQAGRDVTFLVRPARAAALRANGLR
                     VLSPLGDFAVAPRLVEAAALDETFDIVLVTVKAFGLAAAIEDFAPAVGAGTMILPVLN
                     GMKHIDDLRARFGPDRVLGGVCRVSTTLDAQGRIVHLAPLHELVYGEMDGQRTARIEA
                     LHAFMSDAGFDAHLSTRIAQDLWDKWILLATLGGICALARGNVGEIAASDGGPDFVRA
                     FLAEAAAVAAAAGFAPRPQVVDYTLGVLTAAGSALTSSMYRDLMAGERVEAGQILGDL
                     RDRALRAGVAVPLVSAAYVQLDVHQRRLHGR"
     gene            8129..9553
                     /locus_tag="VIN75_18485"
     CDS             8129..9553
                     /locus_tag="VIN75_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8976208.1"
                     /translation="MSNVLRLSFRQTLLAAFLLIAGLLAAASLGGLFTLERVMAQSRV
                     ATASALLLTADARQLAELRVAMERSARQYIVLDDPSLLRGFTQAADDAARLLGEMRSH
                     DLPPALAAQWLAQVEPIKEQLDGPKASIQWRQDALSVRFRTLDRLNDQIAAEVRRIDD
                     DRSAALQERLEAGRASVGTQVLGAIGLAALLALAFGVWLTRPLKRLERAIAGLGENRM
                     DHPVDIRGPSDMRSLGRRLEWLRLRLKELDEDKARFLRYTSHELKTPLAALREGVALL
                     EDGVAGPLTADQREIARILGQNTATLQGQIEALLRFNAAAFEARRLNRSDTDLRQLLQ
                     ALVEEQRLQWQARHLQIRIDGDAPRLAVDADKLSAAFGNLLSNSIRFSPANGVLSITL
                     SSSPGKVRIDLRDQGPGVAPADRERIFEPFFRGEIQPEGAVRGTGIGLSIVREYVQAH
                     GGRVDLLSDGPGAHFRIELPHAND"
BASE COUNT         1388 a         3594 c         3425 g         1229 t
ORIGIN      
        1 gacgagctgt tcatgcaggc gcccgcgggc atcgcggtgc tggacggccc cgagcatgtc
       61 ttcgagctcg cgaacccggc ctaccacgcg ttcatcggcg gacgcgacat cgtcggcaag
      121 cgcctggtcg acgcgctgcc cgagatgcgc ggccagccct tcccggcgct gctggacgag
      181 gtctaccgca cgggcatcgc ccatgtcggc aacgaggcgc cggtgcagct gctgcggcgg
      241 ccggacggcg ccgtcgagga ggcgttcttc aatttcgtct accagccgct gcgcgacggc
      301 cgcggcctca cctcgggcat cctcgtgttc gcctacgagg tcaccgagca ggtgaacgcg
      361 cgccggcatg tcgagcacct ggccgacgag ctgcgcgcgg agcaccggcg caaggacgac
      421 ttcctcgcga tgctggcgca cgagctgcgc aacccgcttg cgccggtcag cgcggcggcc
      481 gaggtgctgc gcctgggcca cgccaacgac ccgcgcctgc gccgcaccag cgagatcgtc
      541 acgcgccagg tgcgccacat ggccacgctg atcgacgacc tgctcgacgc gtcgcgcgtc
      601 acgcgcggac tcgtctcgat cgcgagcagc tgcgaggacc tgcgcgacat catcgccgcc
      661 gccatcgaac aggcggcgcc gctgatggac gtgcgccgac atcggctcac ggtctcgacc
      721 ggcagcgaat tcgtgtgcgt gctgggcgac cgcaagcggc tcgtgcaggc ggtggccaac
      781 ctgctggaca acgcggccaa gtacacggcc gagggtggca gcatcgagct gacgctggac
      841 cacgacgacc gcgaggccac gctgcacgtg cgcgacaacg gcatgggcgt cccgccggag
      901 atgcgcgagc gcatcttcga tctgttcgtc cagggccagc gtggcgccga tcgcgcgcag
      961 ggcggcctgg gcatcgggct ggcgctggcg cgccggctcg tggagctgca tggcggttcg
     1021 ctggcctgca tcgacacgcc atccggccat ggcagcgact tccggatccg gctgccgctc
     1081 gcaccggcat cggcgccctg cgcgtcccgc gcgcgtgccg ccgcacgcga ggaggcggtc
     1141 ggcgccgcca ggccgaccat cctgatcgtc gacgacaacg acgacgccgc acgcatgctc
     1201 gcgatgctgc tcgaggacag cggctacgag gtcatgaccg agaacgatcc ggtcgccgcg
     1261 ctcgaacgcg ccgcccgcac gcggcccgac gtcttcctgc tggacatcgg cctgccgcgc
     1321 atggacggcc acgagctggc gcgccggctg cgcgcgacgg acgcggggcg cgatgccacg
     1381 ctggtggcgc tgaccggcta cggccaggcc accgagcgcg agaaggcggc gcaggccggc
     1441 ttcgaccact acatggtcaa gccgcccgat ccggtggcct tgcgccagct gctgggctcg
     1501 gtggtgccgg cgtcggcctg aacgggccgc cttagcgcgc gaaggtcgtg gtgcgcgcga
     1561 gcagcgtcga cagctcgttc attcgcagcg gcttgctcag gtgggtgtcg aacagctcgt
     1621 gttccgagcg cagcggctcg tcctgcatct tcagcgccga cagcgaggcc agcatcggcg
     1681 ggcggacgtc gtcgtcgtcg cggatccgcc gcgccgccga gcagccgtcg agcacgggca
     1741 tctgcacgtc caggatggcg aggtcgggat gcaggtcgtg gcaggcccgc accgcctgct
     1801 cgccatcgta gaccgcgacc gcgtcatagc ccatcaggcc gagcagctgg gccagcgagt
     1861 cggcggcgtc acgattgtcg tcggcgacga cgatgcagga acgggtcggc atgggtcgag
     1921 cggggaggcg gggcggtcgt ggtggatcga gcaatctccg ctcccgcggg acgaaagact
     1981 tgtaactcaa tgcgcggccc agttcgcctg cagccacatc acggcgccga cctcgacggc
     2041 gtcgcccacg ccgtgcgcga ccacgggcgc gagcaggctg cgcgagcgct ccatcagcca
     2101 gacgtagatc aggccgaacg cgaacgcgta cagctgctgg gcgatcgcca gcgacagcgg
     2161 gtcgacgcgg aacgactcgt agtgcgcgag gccgaacagc aacgccacca gcacgcccga
     2221 cacgggcagg tcgacccggc cgacgcgcac gcggccgggc accagcgcgg tcagcaggcc
     2281 caccagcagg ccgcggaaga tcggctcctc gcacaggccc gagcaggcca tcacgacgag
     2341 ccagccggcg acgccgccat gcgacgggtc gtagcccgct tccggcgcgc gatgggcggc
     2401 cagctgcggc caccagtcgg ccaccagcat cagcacgccc atgcccacgc cgatgaccag
     2461 cgccaggccg acgagcgagc gcccgcgcgc gggccaggcg aggtaggtgg gggcctgcgg
     2521 cagcacgcgc cgcatcaccg cgatgcagcc gaggccggcc agggtctgga acaccatcgc
     2581 caggccgacg aaggcccaga cgtgcgcgtt gaagaacggc gccgcctggt gcgcgatcag
     2641 ccgcgccagc tcgcgcgcgg gccacagcac gccctgcatc aggacgccgg cgagcagcac
     2701 gggccagagg cgccagcgcg ggacgtcgac ctggacgaag ggccggtggg gagcggtcag
     2761 ggtggtcgtc atggcgggcg gtcctcgagt cggcgatggc cgcactctag ggacgcgcac
     2821 ccgcggcgcg agggccgatg cgacgaggcg ccggatccgc ggcccgagct cacgccgcgc
     2881 ggcgccgaag cgcgaggccg gcgtgtcggc gccgtccgac aacgcgtcgc gtccgccgcc
     2941 taggcccacg cgaccgtggc cgccggaaga tgcgggccag cgatccatca cttccatcat
     3001 cgagacgcac gacgctccat ggccaagatc ctcttcgtcg acgactcccg cgacagcgcc
     3061 gactcgctcg cgaccttctt caagctgctg ggacacgaga ccgaggtcgc ctacgacggc
     3121 gacgcggccg tcgagctctc caccgcgttc cgccccgaca ttgcgttcat cgacctgcag
     3181 atgcccatgc tggacggctt cgacaccgcc cgcgaattcc gctcgcgcct gggcgagcgg
     3241 cccgtgctga tcgcgttgac cggccaggaa tgggaacgca ccggcgacgc cgcggaccgc
     3301 gcgggcttcg acggctacct gcagaagccg gcgcaggcat cgcagctggc caagctggtg
     3361 gacaacctgg cgtcgtgagc ggtggcctgc ccgtcatcct gcgactgcac gcaggatgac
     3421 accgggtggg cgcgaagcgg cagcaggacg tcgcgccgag cgccgcccct tcaggccccg
     3481 tccagcgccg cgttcaccgt ctcgacgaac cgcgccggct ccaccggctt ggccagcagc
     3541 gcgacgcccg gcggcaggcc gccgagcttc tcgacatggg cgggcccgtg cgacgacacc
     3601 gccacgatgg ccggcggccg ggcgtggccc ggcgcggaca ggtggcggat catctgcacg
     3661 ccgtcgatgt agggcatcga gatgtcggtg acgatgagat cgggcatcgc cttgcccacc
     3721 tcgatcagcg cgtcgaagcc gttgtcggcg caggtgatgc gcgcgcccgg cacggcgatc
     3781 tcgaccaacg cctgcagcag gtcgcgatcg tcggcgctgt cgtcgacgac cagcaccgtg
     3841 aacggcacga cgacctcggc ggcggccccc gtggccgccc cgccccgctg ctgcatctgg
     3901 gcgcggatga actcctcggc gctgtcggcg tcgatgcggc gatggccgcc gaccgtcttc
     3961 caggccttca ggtggcccag gtcgacccag cgctgcaccg tcggcacgga cacgcccagc
     4021 cggcgcgcca ggtgggcggt cgaatacgag ggaggccccg ccggggcgcg cgaattcgcc
     4081 atcggctcag tttagcgaca agccgccgcg caacgcacgg ttttcgtgcg tcatccgtca
     4141 ctgacgttcc ggcggcgcga cgtcctgcat gtggcgcgcg acctcgacgt cgatctcggc
     4201 ctgcaccgcc tccagacgcc cgagcagcgc ccgccagcgc tcgggcaggc ccaggtcgac
     4261 ggcgtcgtgc aactcgcgcg cgagcgccgc cagcgccggc gcgcccagca tcgacgcggt
     4321 cgacaccagc gtgtgcgcga tgcgggcgcc gacctccagc tcgccggcgt cgagcgcggc
     4381 gcggatctcg ttgcccgacg tctcgcgctg cttcaggtag cgcgcgagga tcttgtgata
     4441 cagctccggc cggcccaggc accggcgcag gcccagctcg aagtcgacga ccgggccgga
     4501 ggccggcggg cgctcggcca ccggtgcagc caccccgacg ggcccggccg gcgtcagcca
     4561 gcgcagcatc acctcgaaca gctgcgccgg ctcgaacggc ttgttgacgt ggtcgttcat
     4621 gccggccgcg gtgctcttgt cgcgatcgcc ggccatcgcg tgcgccgtca tcgcgatcac
     4681 cggcagcttc gcgaacgcct ggtcggcgcg gatcagccgc gtgcactgca ggccgtccat
     4741 gcccggcatc tggatgtcca tcagcaccag gtcgaagcgc gcctcgcgca gttgcgccag
     4801 cgcctcgtgg ccgttgcccg ccagcgtgac gtccatgccc gcgacgtcgc acagcagctc
     4861 ggtggcgacg aagcgattga gctcgttgtc ctcgaccagc agcacgcggc gtccgcgcag
     4921 cgcctcgggc gcccgcgcgc gcggggccgc gccgcgcgcg ggcgcagccg ccggcgcagg
     4981 cgcggggccg ccgcgctgcg ggccgcaggc gccattgatc gcgtccagca gcgtggaggc
     5041 gccgaccggc ttggccaggc agcccgcgac gccctgcctg gccgcctcgc gcacgatcgc
     5101 ctcgtcgccg taggccgtca ccagcaggat ggccggcgcc ggtcccggca gcggatcctc
     5161 gcgcagcatg cgcatgacgt cgaagccgtc cagtcccggc atgcgccaat cgagcagcac
     5221 cagctggtac ggccggccga cgcccgccgc ccggcgcagc tccgccaggc ccgcggcgcc
     5281 gctggcctcg accaccggcg cgtagcccag cagcacgagc aggtcgttga ggatctcgcg
     5341 cgagttggcg ctgtcgtcga ccaccaggat gcgcaggtcg cggtgcgcgc cggcttgcgg
     5401 cagccgcgcg gcctgcgtcg ggttgcccac gcgaaaacgc gcggtgaagt ggaagtcgct
     5461 gcccaggccg ggcgcgctct cgacgccgat ctcgccgtcc atcagtccga cgagcttgcg
     5521 gcagatcgcc agccccaggc cggcgccgcc gtgcacgcgc gtgctcgacg cgtcgacctg
     5581 cgagaacggc cggaacaggt cctcgatctg cgccggggcg agcccgatgc cggtgtcctt
     5641 gacgtcgaag cgcagcagca ccgagtccgc ctggctggcg acgctgcgca cggtgacgac
     5701 gacgatctcg cccttgtcgg tgaacttgac ggcgttgctg cacaggttga ccagcacctg
     5761 cagcagccgc atcggatcac ccaccagcac cggcggcacc tcgggcgagg tcttgagcag
     5821 cagctccagg ccctttctct gcgccttcag gccgatcagc gcggtgacgc ggtcgaacac
     5881 ctcgtcgagc gagaagtcgc gcgactcgag caccaggcgg ccggcctcga tcttcgagaa
     5941 gtcgagaatg tcgtcgatca cgagcagcag cgcctcggcg gccgcgcgcg cctgcccgag
     6001 gtagccgcgc tgcttgtcgg tgagctcggt gcgcagcgcg aggtccgtca tgccgaggat
     6061 cgcgttcatc ggcgtgcgga tctcgtggct catgttggcc aggaattcgc tcttggcgcg
     6121 cgtggccgac cgcgcctcga cgagcgccga ctccaggtcg tggcgctggc cttcgacggt
     6181 gtcgacggcg ctcgcgagac gtgcgcgcat gcggtcgatc gccaccagga tctggtcgaa
     6241 ctcgtcgccg ccggccgcca cgaccggcgt gcgcgtgtcc ccggcgccga tgcgctcgag
     6301 gacgccgccg acctcggcca cgcgctcgac gatcggcttc atcagcacgc gccgcacgat
     6361 cacgaacgcg gcaagcgcca gcgcgatcca gcacgccagc atcatgatgc gcacacgctc
     6421 gacgaaggtg gccgcgctcg cctcgcgctg cgcgttctcg cgctgcgcgg cggcgacggc
     6481 gaactcgatg cggtcgcgat ggtcggcgaa cagcggctgc agcgtgtgcg cgagcagctc
     6541 gcgcgcggtg acgatgtcgt tgcggtgcag cgcgggcagc aggtcgcgct cgaccacctg
     6601 gaagtacgtg gccgccggcg cgcaggccgc gtccaccagc gagtccttca cggcgcccgg
     6661 cggcagcgtg cgcgcccact cggcgcggcg cgtccggaac tcgctctcgc gctcgtgcag
     6721 cagcgcctcg agcagcccca cgcgggccgt gtcgccgtgc gcctgcgcat cgaccagctc
     6781 gaacaccgtc aggtggctct cgacgatgaa cagcggcggc gggaggatgt cggcgacgac
     6841 ggcctggccg ccgtcgatgg ccttgtacag cgggccgttg atgcgcaccc gctcgaacgc
     6901 gaggtccagc cccagcagca ccgccagcgt gaccaccacc atcccgacat tgaacgcaag
     6961 gagcttcgtg cgcagggaca ggcggccagg atgcatgcga ggtctctcgg tcaaggactc
     7021 acgtgagcgc cggatgcccc ggcgtggcac acgggatcga gtctaacgcc catgcagccg
     7081 gcgctggtgg acgtcgagct ggacataggc cgccgacacc agcggcaccg ccacgcccgc
     7141 gcgcagcgcg cggtcgcgca ggtcgcccag gatctggccg gcctcgacgc gctcgccggc
     7201 catcaggtcg cggtacatcg acgaggtgag cgcggaaccc gcggcggtga gcacgcccag
     7261 cgtgtagtcg acgacctgcg gccgcggcgc gaagccggcc gcggccgcga cggccgcggc
     7321 ctcggccagg aacgcgcgca cgaagtcggg gccgccgtcg gacgccgcga tctcgccgac
     7381 gttgccgcgc gccagtgcgc agatgccgcc cagcgtggcc agcaggatcc acttgtccca
     7441 caggtcctgc gcgatgcgcg tgctcaggtg cgcgtcgaag ccggcgtcgc tcatgaacgc
     7501 gtgcagcgcc tcgatgcgcg ccgtgcgctg gccgtccatc tcgccgtaca ccagctcgtg
     7561 cagcggcgcc aggtgcacga tgcggccctg cgcgtcgagc gtcgtcgaga cgcggcacac
     7621 gccgcccagc acgcggtccg gcccgaagcg cgcgcgcagg tcgtcgatgt gcttcatgcc
     7681 gttgagcacg ggcaggatca tcgtgccggc ccccaccgcg ggcgcgaagt cctcgatggc
     7741 ggcggccagg ccgaaggcct tgacggtgac cagcacgatg tcgaaggtct cgtcgagcgc
     7801 ggccgcctcc acgaggcgcg gcgcgacggc gaagtcgccc agcgggctca gcacgcgcag
     7861 cccgttcgcg cgcagcgcgg cggcgcgcgc ggggcgcacc aggaacgtca cgtcgcgtcc
     7921 ggcctgcacc agccgcgcgc cgaagtagcc gccggtcgag ccggcgccga cgatcaggat
     7981 tctcatgact ggccctccgc ggcctcgtgg gcccgccaca ggataggcca acgcgaccgc
     8041 gcgtgcagcc ccggccggaa tgacccgcac accatgcgcg gcgcgctcgc gtgacaatgc
     8101 gcgcttcgcc tcgtcgtacc gcacgccctt gtccaacgtc ctgcgcctgt cgttccggca
     8161 gaccctgctc gccgccttcc tgctgatcgc gggcctgctc gcggccgcgt cgctgggcgg
     8221 cctgttcacg ctcgagcgcg tgatggcgca gagccgcgtc gccaccgcca gcgcgctgct
     8281 gctgaccgcc gatgcgcgcc agctggccga actgcgcgtg gcgatggagc gcagcgcgcg
     8341 ccagtacatc gtgctggacg acccgtcgct gctgcgcggc ttcacgcagg ccgccgacga
     8401 cgccgcgcgc ctgttgggcg agatgcgcag ccatgacctg ccccccgcgc tggccgcgca
     8461 atggctcgcc caggtcgagc ctatcaagga acagctcgac gggcccaagg cctcgatcca
     8521 gtggcgccag gacgcgctgt ccgtgcgctt tcgcaccctc gaccgcctga acgaccagat
     8581 cgccgcggag gtgcgccgca tcgacgacga ccgcagcgcg gcgctccagg aacggctgga
     8641 agcgggccgc gcgtcggtgg gcacgcaggt gctgggcgcc atcgggctgg ccgcgctgct
     8701 ggcgctggcg ttcggcgtgt ggctcacgcg cccgctcaag cgcctggagc gcgcgatcgc
     8761 cgggctcggc gagaatcgca tggaccaccc ggtcgacatc cgcggcccga gcgacatgcg
     8821 ctcgctcggc cgccgcctcg agtggctgcg cctgcgcctg aaggaactcg acgaggacaa
     8881 ggcccgcttc ctgcgctaca cgtcgcacga gctcaagacg ccgctggccg cgctgcgcga
     8941 gggcgtcgcg ctgctcgagg acggcgtggc cggcccgctg accgccgacc agcgcgagat
     9001 cgcgcgcatc ctcggccaga acaccgccac gctgcagggc cagatcgagg cgctgctgcg
     9061 cttcaatgcc gcggccttcg aggcgcgccg cctgaatcgc agcgacaccg acctgcgcca
     9121 gctgctgcag gcgctcgtcg aggaacagcg gctgcagtgg caggcgcgcc acctgcagat
     9181 ccgcatcgac ggcgacgcgc cgcggctggc cgtcgacgcc gacaagctgt cggcggcgtt
     9241 cggcaacttg ttgtccaatt cgatacgctt cagccccgcc aacggggtcc tgtccatcac
     9301 gctgtcgtcg tcccccggaa aggtgcgcat cgacctgcgc gaccagggcc ccggggtggc
     9361 gcccgccgac cgcgagcgca tcttcgagcc gttcttccgc ggcgagatcc agcccgaggg
     9421 cgcggtgcgc ggcaccggca tcgggctgtc catcgtgcgc gaatacgtgc aggcgcacgg
     9481 cggccgcgtc gacctgctgt ccgacgggcc cggtgcccat ttccgaatcg agcttcccca
     9541 tgcgaatgac tgacgcctcc tcccagcgcg cccgcgcgct cgccggcctg gccgccgcca
     9601 ccgccgcgct ggccctggcc ggctgcgccg tgccca
//
LOCUS       DATCRO010000682         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_581614, whole genome shotgun sequence.
ACCESSION   DATCRO010000682 DATCRO010000000
VERSION     DATCRO010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581614"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VIN75_18490"
     CDS             complement(<1..1346)
                     /locus_tag="VIN75_18490"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY8976209.1"
                     /translation="MTRNTRLAAALVCAAAGTLAGSAHAAGEARNVIFFLGDGMGPVT
                     VTAARIYKGEKQLAAQPGSLVSPERANLVMQSLPYAARVKTFSLDGQTTDSAPSMAAY
                     MTGVKMRNEVISMSSDTTAVDGTGKQYINGEDTTCPPTNGKAVDTLLELAKAKGRSVG
                     AVSTTRVGHATPATTYSHICNRNGYNTIAEQSVPGHANYNPKLLDGVDVLLGGGQRNY
                     LPVATNPGSKRTDGVDLVAAMKAKGYTYVDTGTKLKAIDTAATGKLLGLFSQSEMAYE
                     LDRVKQSLDQPSLSEMTEKALGVLSKNDKGFFLMVEGGRIDHALHGTNAKRALEDTLA
                     FDTAIQTALDFMNKKDPGLKNTLVVVTADHDHAIAFNGYPKIGNPILGLVNGYADGKP
                     SKAADGKPYTALVFGNGGRPGSISYGEANDPNATPDSIAKKINDSKVNSEDSKKPW"
     gene            complement(1403..2887)
                     /locus_tag="VIN75_18495"
     CDS             complement(1403..2887)
                     /locus_tag="VIN75_18495"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY8976210.1"
                     /translation="MTQRLIAPALLGVSLLLSACGGSSSNNGIVLPFIPPPTNTTTPP
                     PAAQAPKNVLFFLGDGMGITTMTAARIYKVGEDGDLTMDTLPETAFVHTYSNDAQVTD
                     SAPSMSAYMTGVKMNNEVISMSAETKATDASGKAYHVNFDSTCPSGNGQAVETLLEQS
                     KARGYATGVVTTTRITHATPAATYSHVCHRDAENTIAAALVPSGAGYNGKLGDGVDVF
                     FGGGTDYFTAKEDGGKRNDKRNLIAELKAAKYSFAANKSEFDKLPADGATKIAGLFTK
                     SHMAYDLDRDATKEPSLAEMTGKAIDALAARKKGFFLMVEGGRIDHALHATNARRALQ
                     DTGAFDDAIKLALDRMQAIDPGLKNTLVVVTADHDHTLQLNGYAARTGRTEAGKPGVL
                     GLVKDYVDTSKTSKDVDGNPYTIIGFGNGPRRLATRGPISDAELESKDYRQEAVVPMD
                     AASDSETHGGADVFLGAQGLGADNFSGVITNTDVFGLIKKAVGL"
     gene            3147..3851
                     /gene="queC"
                     /locus_tag="VIN75_18500"
     CDS             3147..3851
                     /gene="queC"
                     /locus_tag="VIN75_18500"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY8976211.1"
                     /translation="MPLHTTALVLFSGGQDSTTCLADALSKYQRVETLGFDYGQRHRV
                     ELDVRGSILAKMRERFPDWAPRLGEDHVLTLAALAQLGGSSLTEEVAFAMQADGLPNT
                     FVPGRNLLFLTLAGALAYRRGLQVIVTGVCETDFSGYPDCRDDTMKAMQLALSLGLER
                     RLVIETPLMWIDKAETWSLAHRLGGKPLVDLIVEETHTCYLGDREHRYAWGYGCGECP
                     ACDLRRKGWERYAAAG"
     gene            complement(3833..>4338)
                     /locus_tag="VIN75_18505"
     CDS             complement(3833..>4338)
                     /locus_tag="VIN75_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8976212.1"
                     /translation="FSKGKLQVPENVAFMPQRPYVPDGRLRDALTYPNPAANFSDDQL
                     RQALDDALLPDLVDRLDDSDAWSQKLSGGEQQRLAIARVLLKKPAWLFADEVTSALDA
                     QAESVLYKRLADTVKAAGGAMVSIAHRAAVGDFHNQRWTLVPQEEGKDGGARYRLSTS
                     GGQPAAA"
BASE COUNT          567 a         1369 c         1552 g          850 t
ORIGIN      
        1 atccacggct tcttcgagtc ttccgagttc accttgctgt cattgatctt cttggcgatg
       61 ctgtccggcg tcgcgttggg gtcgttcgct tctccgtagg agatcgaacc aggccgtccg
      121 ccgttgccga acaccagagc ggtgtatggc ttgccatcgg ctgccttcga gggcttgcca
      181 tcggcgtagc cattgaccag gcccaggatc ggattgccga tcttcggata gccgttgaag
      241 gcgatcgcgt ggtcgtggtc ggcggtgacc accaccagcg tgttcttcag gccggggtcc
      301 ttcttgttca tgaagtcgag cgcagtctgg atggcggtgt cgaaggccag cgtgtcttcc
      361 agcgcgcgct tggcgttggt gccgtgcagc gcgtggtcga tgcggccgcc ttccaccatc
      421 aggaagaagc ccttgtcgtt cttgctgagc acgccgagcg ccttctcggt catctccgag
      481 aggctgggct ggtcgaggct ctgcttcacg cggtcgagct cgtaggccat ctcgctctgg
      541 gagaacagcc ccagcagctt gccggtggcg gccgtgtcga tggccttgag cttggtgccg
      601 gtgtcgacgt aggtgtagcc cttggccttc atcgcggcca ccaggtcgac gccgtcggtg
      661 cgcttgctgc cggggttggt cgccaccggc aggtagttgc gctggccgcc gcccaggagc
      721 acgtccacgc cgtcgagcag cttggggttg tagttggcgt ggcccggcac gctctgctcg
      781 gcgatggtgt tgtagccatt gcggttgcag atgtgcgagt aggtggtggc cggcgtggcg
      841 tggcccacgc gcgtggtcga cacggcaccc accgaacggc ccttggcctt ggcgagttcg
      901 agcagcgtgt cgacggcctt gccgttggtg ggcgggcagg tggtgtcctc gccgttgatg
      961 tactgcttgc ccgtgccgtc gaccgcggtg gtgtcgctgg acatcgagat gacttcgttg
     1021 cgcatcttca cgccggtcat gtacgcggcc atggagggcg cgctgtcggt ggtctggccg
     1081 tcgagcgaga aggtcttcac gcgcgcggcg tacggcagcg actgcatgac gaggttggcg
     1141 cgctcggggc tcaccagcga tccgggctgg gcggcaagct gcttctcgcc cttgtagatg
     1201 cgcgcggcgg tcacggtgac ggggcccatg ccgtcgccga ggaagaagat gacgttcctg
     1261 gcttcgccgg cggcgtgggc gctgccggcc agggtgccgg cagcagcgca gacgagggcg
     1321 gcggccaggc gggtgttgcg ggtgatgcgg ttcatggtcg tcgtgttcat ggtggtgttc
     1381 gtcgatgagc gctcgctgcc cttcacaggc cgaccgcctt cttgatgagg ccgaacacgt
     1441 cggtgttggt gatcacgccg gagaagttgt cggcacccag gccctgggca ccgaggaaga
     1501 cgtcggcgcc gccgtgggtt tcggagtcgc tggccgcatc catcggcacc acggcttcct
     1561 gacggtagtc cttgctttcc agctcggcgt cggagatcgg gcctcgcgtg gcgaggcggc
     1621 gcgggccgtt gccgaagccg atgatggtgt aggggttgcc atcgacgtcc ttgctggtct
     1681 tgctggtgtc gacgtagtcc ttcacgaggc ccagcacgcc gggcttgccc gcctctgtcc
     1741 tgccggtgcg cgcggcgtag ccgttgagct gcagcgtatg gtcgtggtcg gccgtgacca
     1801 ccaccagcgt gttcttcagg ccggggtcga tggcctgcat cctgtcgagc gcgagcttga
     1861 tggcgtcgtc gaaggcgccc gtgtcctgca gcgcgcggcg tgcgttggtg gcgtgcagcg
     1921 cgtggtcgat gcgcccgcct tccaccatca ggaagaagcc cttcttgcgc gcggccagcg
     1981 cgtcgatggc cttgccggtc atctcggcca ggctcggctc cttggtggcg tcgcgatcga
     2041 ggtcgtacgc catgtggctc ttggtgaaga ggccggcgat cttggtggcg ccgtcggccg
     2101 gcagcttgtc gaactcgctc ttgttcgcgg cgaagctgta cttcgcggcc ttcagttcgg
     2161 cgatgaggtt gcgcttgtcg ttgcgcttgc cgccgtcttc cttggcggtg aagtagtcgg
     2221 tgccgccgcc gaagaacacg tcgaccccgt cgcccagctt gccgttgtag ccggcgccgc
     2281 tcggcacgag cgcggcggcg atggtgttct cggcgtcgcg gtggcacacg tgcgagtacg
     2341 tggccgcggg cgtggcgtgc gtgatgcggg tggtggtgac cacgccggtg gcgtagcccc
     2401 tggccttgct ctgctcgagc agcgtttcca ccgcctggcc gttgcccgag gggcaggtgc
     2461 tgtcgaagtt gacgtggtag gccttgcccg aggcgtcggt ggccttggtc tcggcgctca
     2521 tcgagatgac ttcgttgttc atcttcacgc cggtcatgta ggccgacatc gaaggagcgc
     2581 tgtcggtgac ctgggcgtcg ttcgaatagg tgtggacgaa ggccgtttcg ggcagcgtgt
     2641 ccatcgtgag atcgccgtcc tcgccgacct tgtagatgcg cgcggcggtc atggtggtga
     2701 tgcccatgcc gtcgcccagg aagaacagga cgttcttcgg cgcctgcgct gcgggtggcg
     2761 gcgtggtggt gttggtgggc ggcgggatga acggcagcac gatgccgttg ttgctgctgc
     2821 tgccgccgca ggcggacagc aggagagaga cgcccagcag tgctggggcg atgaggcgtt
     2881 gggtcatgat tccctcggtt ggttgttgga ccggcgcacc gggcgtgcgc catttctggc
     2941 gatgaaatct aaagacgtca gatgacccgg ccgtgacgag ttgaaagccg aatgaaagtt
     3001 cgccgatcgc tcggaaataa atgcccggaa tgtcttcggc gtttcatgcg catcggctag
     3061 cggatgcggt gaattccggc agctgcgcca agctgacgga aaatcgcgtc tttggattgc
     3121 ggcggcacgc accgccgccc acggacatgc ctctgcacac caccgccctc gtcctgttct
     3181 ccggcggcca ggactcgacc acctgcctcg ccgatgcgct ctcgaagtac cagcgcgtcg
     3241 aaaccctggg cttcgactac gggcagcgcc accgcgtcga gctcgacgtg cgcggcagca
     3301 tcctcgcgaa gatgcgcgag cgctttcccg actgggcgcc tcgcctgggc gaagaccacg
     3361 tgctcacgct cgccgcgctg gcgcagctgg gcggttcgtc gctcacggaa gaggtcgcct
     3421 tcgccatgca ggccgacgga ctgcccaaca ccttcgtgcc ggggcgcaac ctgctgtttc
     3481 tcacgctcgc gggggcgctg gcctaccggc gcgggctgca ggtgatcgtc accggcgtct
     3541 gcgagaccga cttctcgggc taccccgact gccgcgacga caccatgaag gcgatgcagc
     3601 tcgcgctctc gctcgggctg gagcgccgcc tcgtcatcga gacgccgctc atgtggatcg
     3661 acaaggccga gacctggagc ctggcgcacc gcctgggcgg caagccgctg gtcgacctca
     3721 tcgtggaaga aacccacacc tgctacctcg gcgaccgcga gcatcgctac gcctggggct
     3781 acggctgcgg cgaatgcccc gcctgcgatc tgcgcaggaa gggctgggag cgctacgccg
     3841 cggcgggctg acccccggag gtgctcaggc ggtagcgcgc gccgccgtcc tttccttctt
     3901 cctgcggcac gagcgtccag cgctggttgt ggaagtcgcc caccgccgcg cggtgcgcga
     3961 tcgacaccat cgcgccgccc gccgccttca ccgtgtcggc aaggcgcttg tagagcacgc
     4021 tctcggcctg cgcgtcgagc gcgctggtga cctcgtcggc gaagagccac gccggtttct
     4081 tcagcagcac gcgcgcgatg gccagccgct gctgctcgcc gcccgagagc ttctggctcc
     4141 aggcgtcgct gtcgtcgaga cgatccacca ggtcgggcag cagggcgtcg tccagcgcct
     4201 ggcgcagctg gtcgtcgctg aagttcgcgg ccggattggg gtaggtcagt gcgtcgcgca
     4261 gcctgccatc gggcacgtac gggcgctgcg gcatgaacgc gacgttctcg ggcacctgca
     4321 gcttgccctt cgagaagg
//
LOCUS       DATCRO010000683         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_41365, whole genome shotgun sequence.
ACCESSION   DATCRO010000683 DATCRO010000000
VERSION     DATCRO010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41365"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VIN75_18510"
     CDS             <1..982
                     /locus_tag="VIN75_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin"
                     /protein_id="HEY8976213.1"
                     /translation="AGAPYIVINSAGDIGTWVHTNLATKPTEGPSSQWSNGYSADVAW
                     SDALYIAGIGAINGTSSGGFGFWSGDGGKTWTSFAANAPGGAAGSGGSVAVTARNKLV
                     WAPNDTVPSYTTDNGASWVATNLPALPSVGTGIGRSYHLAADRKNANKVYAYEAGGAW
                     WAGAGKVYVSTDGGHSFTLSQASVDAKLAPNSFWATSLAVNPNAEGDVWLADGNAVYH
                     SLDSGATWTKLGAFASIWGSNAWPDVQGATTIALGKAKAGASYSAAVYVVGVVNGVWG
                     VHRSDDGGATWTRFNDDAHQYGGIGAIAADQNTYGRIYAAGNGRGLFYSN"
     gene            1170..>3125
                     /locus_tag="VIN75_18515"
     CDS             1170..>3125
                     /locus_tag="VIN75_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin"
                     /protein_id="HEY8976214.1"
                     /translation="MRLQLSTLMLAIGAGSALPSIAQTCSPTTITPYVKVNGTWIQTS
                     SATLKTGASATFGPQPVSGGSWSWSGCGTGGASREQTVTPAAACTANVTYTNACGAKS
                     QQAFSIKVGTWASAKFGGGGYVTGLIFHPTSPNVLYARTDVGGAYRWDQASSSWLPIT
                     DGFSAAESFQHGVESIALDPNNDQLVYMVTGMYASADATARLYMSGNRGSSWTSVNLP
                     FSAGGNNVGRAIGERLAVDPNNPSILFYGSRTAGLWKSADSGHTWAQVSSLSSTKLTS
                     AQVSALNGQPIGVEQLVYDTSSKGSGSPTWILWASIAPDYVNAAGLASNLYKSTNGGA
                     TWTPVATPVSGYHIPHMVRTADGEFYVLFTKGTGPGDAGPGRLYKFDASNGSTWTLLA
                     SNDAAGYGGISVYGSGPTARIALGVSGTWGNYAGQQIVQLSDDGGRTWREIEAGMPGQ
                     TASGWVDDIEIDPSHRDHIMHVHGGGIVETRSASASTPTWNASINGIEETCTLALNTP
                     PAGAPYFFLNGSGDVGTWVHTDLTKTPTLSPNKQWNSGNSIDTAWSDPNYIAAAGVIN
                     STNTGVGYWSGDGGNSWASFATLPAGAAANTAGAGNIAVTGRNKAVWAPADSVPSYTT
                     DNGATWTQTNLPPLSPVGINRAYRLVAD"
BASE COUNT          530 a         1150 c         1038 g          407 t
ORIGIN      
        1 ggcgggcgcg ccctacatcg tgatcaacag cgcgggcgac atcgggacgt gggtgcacac
       61 gaacctcgcg accaagccga ccgaaggccc gagcagccag tggagcaacg gctactccgc
      121 ggacgtcgcc tggtccgatg cgctctacat cgccgggatc ggcgcgatca atggaacgag
      181 ctcgggcggc ttcggcttct ggtccggcga tggcggcaag acctggactt cgttcgccgc
      241 caacgcgccg ggcggcgccg ccggatccgg cggaagcgtc gcggtcaccg cgcgcaacaa
      301 gctcgtgtgg gcgccgaacg acacggtgcc gtcgtacacc accgacaacg gcgccagctg
      361 ggtggcgacc aacctgccgg cgctgcccag cgtgggcacc ggcatcgggc gcagctatca
      421 cctggccgcc gaccgcaaga acgcgaacaa ggtctatgcc tacgaggcgg gcggcgcgtg
      481 gtgggcgggc gccggcaagg tctacgtgtc gacggacggc gggcacagct tcacgctgag
      541 ccaggcatcg gtcgatgcca agctggcacc gaactccttc tgggccacct cgctggcggt
      601 caatcccaac gccgagggcg acgtctggct ggccgatggc aacgccgtct accactcgct
      661 cgattcgggc gcgacctgga ccaagctcgg tgccttcgcc tcgatctggg gcagcaacgc
      721 gtggcccgac gtgcagggtg cgaccacgat cgccctgggc aaggccaagg ccggcgcatc
      781 gtactcggcc gcggtctacg tggtgggcgt ggtcaacggc gtctggggcg tgcatcgctc
      841 ggacgacggg ggcgccacct ggacgcgctt caacgacgac gcgcaccagt acggcggcat
      901 cggcgcgatc gcggccgacc agaacaccta cgggcggatc tacgcggcag gcaacggccg
      961 cgggctgttc tacagcaact gaacgcaggc tgccagcgcg cagggcgccc ggccttggcc
     1021 ggcgccccgc cgcgcggctc cgcatcgaga ccaaggaggg tacttcccac cgtcgccgag
     1081 gtcgcgccca ggcgctgagc cggcctcgcg cacaacgctc gtgaccggag gagacaaccg
     1141 gccgagccct tcatctgaaa ggcacaacca tgagactgca gctttcgacg ctgatgctgg
     1201 ccattggcgc cggctcggcc ttgccctcca tcgcccagac gtgctcgccg acgaccatca
     1261 cgccgtacgt caaggtcaac ggcacctgga tccagacgag ctccgccacg ctcaagaccg
     1321 gcgcctcggc caccttcggc ccgcagccgg tgtccggcgg gtcctggagc tggagcggct
     1381 gcggcaccgg cggtgcctcg cgcgaacaga ccgtcacgcc tgccgcggcc tgcaccgcca
     1441 acgtcacgta cacgaacgcg tgcggcgcca agagccagca ggcgttctcg atcaaggtcg
     1501 gcacctgggc cagcgccaag ttcggcggcg gcggctatgt caccggcctc atcttccatc
     1561 cgacgtcgcc gaacgtcctg tacgcgcgca cggacgtggg cggcgcctac cgctgggacc
     1621 aggcgagctc gtcgtggctg ccgatcacgg acggattcag cgcggcggag agcttccagc
     1681 acggcgtcga gagcatcgcc ctcgacccga acaacgacca gctggtctac atggtcacgg
     1741 gcatgtacgc ctcggccgac gccacggcca ggctctacat gtccggcaac cgcggcagca
     1801 gctggacgtc cgtcaacctg cccttctcgg ccggcgggaa caacgtcggc cgggccatcg
     1861 gggagcgcct ggcggtcgat cccaacaatc cgtccatcct gttctacggc tcgcgcacgg
     1921 ccggcctgtg gaagagcgct gactccggcc acacctgggc ccaggtgtcg tcgctgtcgt
     1981 cgaccaagct gacctccgcc caggtcagcg ccctgaacgg ccagccgatc ggcgtcgagc
     2041 agctcgtgta cgacacctcg agcaagggca gcggctcgcc gacctggatc ctctgggcca
     2101 gcatcgcgcc cgactacgtc aacgccgcgg gcctggcctc gaacctgtac aagtccacca
     2161 acggcggcgc cacgtggacg cccgtcgcga ccccggtgtc gggctaccac atcccgcaca
     2221 tggtgcgcac ggccgacggc gagttctacg tcctgttcac caagggcacg ggcccgggcg
     2281 acgccggccc cggccgcctg tacaagttcg acgccagcaa cggcagcacc tggaccttgc
     2341 tggccagcaa cgacgccgcg ggctacggcg gcatctcggt gtatggcagc ggcccgacgg
     2401 cccgcatcgc gctgggggtg agcggcacgt ggggcaacta cgccggacag cagatcgtcc
     2461 agctgtccga cgacggcggc aggacctggc gcgagatcga ggccggcatg ccgggccaga
     2521 ccgcttcggg ctgggtcgac gacatcgaga tcgatccgtc ccaccgcgac cacatcatgc
     2581 acgtgcatgg cggcggcatc gtcgagaccc ggagcgcctc cgcatcgacg cccacctgga
     2641 acgcctcgat caacggcatc gaggaaacct gcaccctggc cctgaacacg ccgccggcgg
     2701 gcgcgccgta cttcttcctc aacggttcgg gcgacgtcgg cacctgggtc cacaccgacc
     2761 tcacgaagac gccgacgctc agccccaaca agcaatggaa cagcggcaat tccatcgaca
     2821 ccgcctggtc cgatccgaac tacatcgcgg cggccggcgt catcaactcg accaacacgg
     2881 gcgtcggcta ctggtcgggc gacggcggca acagctgggc gagcttcgcc acgctgccgg
     2941 ccggcgccgc ggccaacacc gccggagcgg gcaacatcgc ggtcaccggg cgcaacaagg
     3001 ccgtgtgggc gccggccgat tcggtgccgt cctacaccac cgacaacggc gctacctgga
     3061 cccagaccaa cctgccgccg ctgtctccgg ttggcatcaa ccgcgcctac cgcctggtgg
     3121 ccgac
//
LOCUS       DATCRO010000684         5200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_716403, whole genome shotgun sequence.
ACCESSION   DATCRO010000684 DATCRO010000000
VERSION     DATCRO010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716403"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VIN75_18520"
     CDS             <1..1410
                     /locus_tag="VIN75_18520"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8976215.1"
                     /translation="VAFSGENVFSQRLRDVPVRAREIQRRLDRMVWNGRIHQASESSA
                     FSRSLLEEIAATGRRTKDQFERASSQLLATVASGLQSEARLLAELSVDILARSLYERA
                     NDVRWWAADPVLALLDAGTARATLARLNGLYTVYANLLVFDAQGVVIAASRDAGIEGR
                     TLASPWIAECLALRDPMHFAVSPFESGALHDAGTWVFAAPLLVDGRAVGGVAAVFDAT
                     PQVQAMLREALPDAPGSLAAFCLRDGHVVGRTGELHVTLPTSALALAPGQSWSGLLAE
                     AGQCFIVGATAASGYREFRHGEGGVEPVVGVVVIPCGQAVDQHDATPPPVANAPDGVE
                     IATFLIGGQLLGVQAREVVECIEVTAAVRVWRGGFAQRHVGFVTWNDMALPLVDIAAD
                     VGAPGAAHRHAIVLRAGAQAYGLLVSDLGPVADMKLSEERGLTGHGDLARLVAQLARS
                     GSVLLPVLSPEAIFGGPAG"
     gene            complement(1432..3300)
                     /locus_tag="VIN75_18525"
     CDS             complement(1432..3300)
                     /locus_tag="VIN75_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8976216.1"
                     /translation="MPPRFHAPSIIACATVLAFAPASRAQDAAASAPAKKVIMQQTDL
                     PRFSYPISGTATKFVESDGFGAFAAKVGQDIDGVLGGYQIDDKSTLAELLGARLGVQE
                     IAGDYAGALKTVDELRAQQTKPASQALTGLFAKARLQAALDAGGESGPAFEAAFRRHY
                     AEAIAPLPWAIVKDGIKSSYGYSRIASRAADLGDVTTELDPAVAKSGALDSGEAWELI
                     STRVDLKFALPLYAARADVLKAYIAKNDTPVPEIWSAREVTLTAADKLTPVNVAIWDS
                     GIDVALFPKQLFTDPKPTASGAHGLAFDDYGNPSKAWTYPLSAAQKAAYPAFQQEVRG
                     RLDLQNGIDSPDALAVQKKYRTMSPDEMHAMEEFDKVLGFYVHGTHCAGIAVRGNPAA
                     RLVVARFDDQLPDFPFAPTEAWVRKIGGDFQQMADYFRTRHVRVVNMSWGDDVAEFET
                     WLSKTGGGADAAARKQKASELYALWREAIENAIKSAPDTLFVTAAGNSDSDAGFLGDV
                     PASLHLPNLIAVGAVNQAGDETSFTSHGATVLVHSDGYQVESFVPGGQRLRLSGTSMA
                     SPNVVNLAAKLIALDPTLTPAQTIALIREGATDTPDGRRHLMDEKRSVALLKAGKK"
     gene            complement(3566..4270)
                     /locus_tag="VIN75_18530"
     CDS             complement(3566..4270)
                     /locus_tag="VIN75_18530"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEY8976217.1"
                     /translation="MPETLAPFYALHREAMVSAMRQRLDLAEPKLRERAQLSEIDSLR
                     GEVMDEFEIVLVQMPQVGGAENRMSDFFMRLLGFLAIGRVLRRHGVSTPVIGDIERET
                     YKAQLLTVPETERLASGRQFMSADNQALVRAQAARSRTADHRAAFPDDFVYDFIEPGP
                     GDTFEFGIDYKACGFCKFAARHGDKEILPTLCGLDFDAYAVRGIRLVRTQTLAGGASH
                     CDFRFSRITPGEDEPA"
     gene            4570..>5200
                     /locus_tag="VIN75_18535"
     CDS             4570..>5200
                     /locus_tag="VIN75_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8976218.1"
                     /translation="MATRKSDTTTPDFPRPFTTVDVLIFTVVDDALKVLLVQRPSGGG
                     EPFPGLWALPGGFVDIDRDDDLEACARRKLNEKTGVASPYLEQLGSWGGAARDPRGWS
                     ATHAYFALMPSDGVTLAKGANAADVAWFGVDDPQLARKKLAFDHALILRAGIERLRSK
                     VEYTSLPAYLLAEPFTLPQLQRTYEIVLGRPVDKSGFRTRMLAADFLAEV"
BASE COUNT          729 a         1842 c         1827 g          802 t
ORIGIN      
        1 gtcgcgttca gcggggagaa cgtcttctcg cagcgactgc gcgacgtgcc ggtgcgcgcc
       61 cgcgagatcc agcgccgcct cgaccgcatg gtgtggaacg gccgcatcca ccaggccagc
      121 gagtccagcg cgttctcgcg ctcgctgctg gaggagatcg cggccaccgg ccggcgcacc
      181 aaggaccagt tcgagcgcgc ttcgtcgcaa ctgctggcga ccgtcgcgtc gggcctgcag
      241 tccgaggcgc gcctcctggc cgagctgtcc gtcgacatcc tcgcgcgcag cctctacgag
      301 cgcgccaacg acgtccgctg gtgggccgcc gatccggtcc tcgccctgct ggacgccggc
      361 acggcgcgcg ccacgctcgc gcgcctgaac ggcctgtaca cggtctacgc gaacctgctg
      421 gtgttcgacg cgcagggcgt ggtcatcgcc gcgagccgcg acgccggcat cgagggccga
      481 accctcgcga gtccgtggat cgccgagtgc ctcgcgctgc gcgacccgat gcacttcgcc
      541 gtctcgccct tcgagagcgg cgcgctgcac gacgccggca cctgggtgtt cgccgcgccg
      601 ctgctggtcg acggacgcgc ggtcggcggc gtcgcggccg tgttcgacgc cacgccgcag
      661 gtccaggcca tgctgcgcga ggcgctgccc gatgcgccgg ggtcgctcgc cgcgttctgc
      721 ctgcgcgacg gccacgtcgt cgggcgcacc ggcgagctgc acgtgacgct gcccaccagc
      781 gcgctggcgc tcgccccggg ccagtcctgg tcgggcctgc tggcggaggc cggccagtgc
      841 ttcatcgtcg gcgccacggc cgccagcggc taccgcgagt tccggcacgg cgagggcggc
      901 gtcgaaccgg tggtcggcgt ggtcgtgatc ccgtgcggcc aggccgtcga ccagcacgat
      961 gccacgccgc cgccggtggc caatgcgccg gacggcgtcg agatcgccac cttcctgatc
     1021 ggcgggcagc tgctgggcgt gcaggcccgc gaggtcgtcg aatgcatcga ggtgaccgcg
     1081 gccgtgcgcg tctggcgcgg cggcttcgcg cagcgccacg tcggcttcgt cacctggaac
     1141 gacatggcgc tgccgctggt ggacatcgcc gcggacgtcg gcgcgccggg cgccgcccac
     1201 cgccacgcga tcgtgctgcg cgccggcgcg caagcctacg gcctgctggt gtccgacctg
     1261 ggcccggtgg ccgacatgaa gctcagcgag gagcgtggcc tcaccggcca cggcgacctc
     1321 gcgcgcctgg tcgcgcagct tgcgcgatca gggtcggtgc tcctgccggt gctgtcgccg
     1381 gaggcgatct tcggcgggcc ggcgggttga atccggcgat cgcgcgccga cttacttctt
     1441 gcccgccttg agcagcgcca ccgagcgctt ctcgtccatc aggtggcggc gcccgtcggg
     1501 cgtgtcggtg gcgccctcgc ggatcagcgc aatggtctgc gccggcgtga gcgtcgggtc
     1561 gagcgcgatc agcttggccg ccaggttgac gacgttgggc gacgccatcg acgtgcccga
     1621 cagccgcagg cgctggccgc ccggcacgaa gctctcgacc tggtagccgt cgctgtgcac
     1681 caggacggtg gcgccgtggc tggtgaagga cgtctcgtca ccggcctggt tcacggcgcc
     1741 gaccgcgatg aggttgggca ggtgcagcga ggccggcacg tcgcccagga agccggcgtc
     1801 gctgtcgctg ttgccggccg cggtgacgaa cagcgtgtcg ggcgcggact tgattgcatt
     1861 ctcgatcgcc tcgcgccaca acgcgtacag ctcgctcgcc ttctgcttgc gcgcggcggc
     1921 gtcggcgccg ccgccggtct tggacagcca cgtctcgaac tcggccacgt cgtcgcccca
     1981 gctcatgttg acgacgcgca cgtggcgggt tcggaagtag tcggccatct gctggaaatc
     2041 gccgccgatc ttgcgaaccc aggcctcggt gggcgcgaac gggaagtccg gcagctggtc
     2101 gtcgaagcgc gcgaccacga gacgcgccgc ggggttgccg cgcacggcga tgcccgcgca
     2161 gtgcgtgccg tgcacgtaga agcccagcac cttgtcgaac tcctccatcg cgtgcatctc
     2221 gtcgggcgac atcgtgcggt acttcttctg caccgcgagc gcgtcgggcg agtcgatgcc
     2281 gttctgcagg tcgagccggc cgcgcacctc ctgctggaac gccgggtacg cggccttctg
     2341 cgcggcgctg agcgggtacg tccaggcctt ggacgggttg ccgtagtcgt cgaacgccaa
     2401 gccgtgcgca ccgctggcgg tgggcttggg gtcggtgaac agctgcttgg ggaacagcgc
     2461 cacgtcgatg cccgaatccc agatcgcgac gttgacgggc gtgagcttgt cggcggcggt
     2521 gagggtgacc tcgcgcgccg accagatctc gggcaccggc gtatcgttct tcgcgatgta
     2581 ggccttgagc acgtcggccc gcgcggcgta cagcggcagc gcgaacttca ggtccacgcg
     2641 cgtcgagatc agctcccacg cctcgccgga gtccagcgcg cccgacttgg cgaccgccgg
     2701 gtcgagctcc gtcgtgacgt cgcccaggtc cgccgcgcgg ctcgcgatgc gcgaatagcc
     2761 gtacgagctc ttgatgccgt ccttgacgat cgcccacggc aacggcgcga tcgcctcggc
     2821 gtagtgcctg cggaacgccg cctcgaacgc cgggcccgac tcgccgccgg cgtccagggc
     2881 ggcctgcagg cgcgccttgg cgaacagccc cgtcagcgcc tgcgacgccg gcttggtctg
     2941 ctgcgcgcgc agctcgtcga cggtcttcaa tgcaccggca tagtcgcccg cgatctcctg
     3001 cacgccgagc ctggcgccca gcagctcggc cagggtggac ttgtcgtcga tctggtagcc
     3061 gccgagcacg ccgtcgatgt cctgccccac cttggccgcg aacgcgccga agccgtcgga
     3121 ctcgacgaac ttcgtggccg tgcccgagat cgggtagctg aagcgcggca ggtcggtctg
     3181 ctgcatgatc accttcttcg caggcgcgga ggccgcggca tcctgcgcgc ggctcgctgg
     3241 cgcgaacgcc agcacggtgg cgcaggcaat gatcgacggg gcgtggaatc gtgggggcat
     3301 cggtcggtgt cctgtgggtg gatgatgggt catgccggcg cgcgcggtgg tgtcacgaca
     3361 agctttcacc cacagcgacg gtacgacgag accgccacgc taccgcgccg cgacgcggtg
     3421 ccgccaggat gctgcgatgg gcggtcgaaa tctggtgtcg ggagacgatg acgcgtcgga
     3481 caggccgtcc tcgaccacgg gccgtcttct ggcccgggcc tggtgatccc acgggacgtg
     3541 cgcgagcacc aagccaggcg ctttcctagg cgggctcgtc ctcgcccggc gtgatgcgcg
     3601 agaaccggaa gtcgcaatga ctcgcaccgc cggccagggt ttgcgtgcgc accaggcgga
     3661 tgccgcgtac ggcataggcg tcgaagtcca ggccgcaaag cgtgggcagg atctccttgt
     3721 cgccgtggcg agccgcgaac ttgcagaacc cgcaagcctt gtagtcgatg ccgaactcga
     3781 acgtgtcacc cgggcccggc tcgatgaagt cgtagacgaa atcgtccgga aacgccgccc
     3841 ggtgatcggc cgtgcggcta cgcgcggcct gcgcgcgcac cagcgcctgg ttgtccgccg
     3901 acatgaactg gcgccccgat gcaaggcgct ccgtctccgg cacggtgagc aactgggcct
     3961 tgtaggtctc gcgctcgatg tcgccgatca cgggcgtcga gacgccatgc cgccgcagca
     4021 cgcggccgat cgccaggaaa ccgagcaggc gcatgaagaa atcgctcatg cgattctccg
     4081 cgccgccgac ctgcggcatc tggacaagca cgatctcgaa ttcgtccatc acctccccgc
     4141 gcaggctgtc gatctccgac aactgggcgc gctcgcgcaa cttcggctcg gccaggtcga
     4201 gccgttggcg catggccgac accatcgcct cgcggtgcag agcgtagaag ggagcaagcg
     4261 tctcgggcat ggggactcct cgcggcgatg catccggtgc ctgcactgaa gcgaatcgac
     4321 gtcgcggcct gtcaccgggt catcagaggc ggcgtcacgg acatccgccg gattcggcat
     4381 tctggaacgc accggatgac cctcggcgcg gcatgcagaa catgcgcctg ctgcgccttg
     4441 tccctgccgc tcatggcgca gcggcttcgc atgccggatc cttccgatgc cgcccgatct
     4501 tagttgctta atacaaccaa taaaggtgta cagtacaacc tatcccgccg aaaatcaagc
     4561 agacgcgcca tggccacccg caagtccgac accacgacgc ccgacttccc gcgccccttc
     4621 accaccgtcg acgtcctgat cttcacggtg gtcgacgacg cgttgaaagt gctgctggtt
     4681 cagcggccca gcggcggggg cgagccgttc ccggggttgt gggcactgcc cggcggcttc
     4741 gtcgacatcg accgcgacga cgatctcgag gcctgcgcgc gccgcaagct gaacgagaag
     4801 accggcgtcg cgagccccta cctcgagcag ctgggcagtt ggggcggcgc ggcgcgcgac
     4861 ccgcgcggct ggtcggccac gcatgcctac ttcgcgctga tgccgtcgga cggcgtgacg
     4921 ctggccaagg gcgccaacgc ggccgacgtc gcgtggttcg gcgtggacga tccgcagctc
     4981 gccaggaaga agctcgcgtt cgaccacgcg ctgatcctgc gtgccggcat cgagcggctg
     5041 cgcagcaagg tcgaatacac gtctctgccc gcctacctgc tggccgagcc gttcacgctg
     5101 ccgcagctgc agcgcaccta cgagatcgtg ctgggcaggc ccgtcgacaa gagcggcttc
     5161 cgcacgcgca tgctggcggc cgatttcctg gccgaggtcg
//
LOCUS       DATCRO010000685         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_997628, whole genome shotgun sequence.
ACCESSION   DATCRO010000685 DATCRO010000000
VERSION     DATCRO010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997628"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VIN75_18540"
     CDS             complement(<1..811)
                     /locus_tag="VIN75_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8976219.1"
                     /translation="MQTFRIAAVSDIHGNLPALEAVWAEIERASPTMVVNLGDIASGP
                     LWPGETVQWLMARAAAEPDRWRTIAGNHERQVLAPDVARMGASDAYAARALGASERAW
                     LAALPPTHWLADDVLLCHGTPASDLVYFMETVIPGFGVDGQRGMRAATAVELAARAGE
                     HPPGDTRAGLASALLLCGHTHVPRAMAVPGGPLLVNPGSVGLQAYEDDHPFAHVVETG
                     SPDARWALLERDRRGAWHVQLRATPYDWAAAAARAEANGRGDWADALATGFV"
     gene            1067..1474
                     /locus_tag="VIN75_18545"
     CDS             1067..1474
                     /locus_tag="VIN75_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976220.1"
                     /translation="MTLDSHSRIAAVLHIGLGALSLCVLLVIGAMAAAVGVYGETFGL
                     DKELGAMIGGIGMIVVISFALFAVLEIVGAVMLMRGNDSGRVITLVFSVLHLVNIPIG
                     TAIGGYSLWALLRNAPQPMPAATSMPSGRAGPV"
     gene            1694..2107
                     /locus_tag="VIN75_18550"
     CDS             1694..2107
                     /locus_tag="VIN75_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976221.1"
                     /translation="MPVIDTHVRVAAWLHIVQGALAVCVIGCIGLIVGFTGVAAGSAV
                     HGRDATIVAWLASLGAVLFAVILAFPLLEIVGGVMLLGGSVAGRIITLIFSVLGLISI
                     PIGTAIGIYSLWALLREAPAAPADAGVVQPGMRST"
     gene            complement(2250..3614)
                     /gene="radA"
                     /locus_tag="VIN75_18555"
     CDS             complement(2250..3614)
                     /gene="radA"
                     /locus_tag="VIN75_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8976222.1"
                     /translation="MAAPKTVFTCSACGGTNPKWLGRCPHCSAWNTLEEGIGEPPGGA
                     SKNRFQALAKSQPVATLADIDATDVARTPTGIEELDRVLGGGIVAGGVVLIGGDPGIG
                     KSTLLLQAVDALATQMKVLYVTGEESSAQVALRSRRLDLPGTQVRVLAEINLERILAT
                     IESEQPAFCVIDSIQTLYSEQLTSAPGSVAQVRECSAQLTRTAKSGGTTIVLVGHVTK
                     DGSLAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNEIGVFAMTERGLKGVA
                     NPSAIFLSTHGEPVPGSCVLVTLEGTRPMLVEIQALVDAGGPSPRRLSVGLERDRLAM
                     LLAVLHRHAGVSTGDQDVFVNAVGGVRISEPAADLAVLLAIQSSIRNRALPRGFLAFG
                     EVGLAGEVRPAPRGQDRLKEAAKLGFSVAIVPKANAPKKPIEGLTIHAVERIEEAIEV
                     LRGL"
     gene            3720..4181
                     /locus_tag="VIN75_18560"
     CDS             3720..4181
                     /locus_tag="VIN75_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976223.1"
                     /translation="MTDLTLRPASLDDLDQLAPLFDAYRRFYEQPGDVARASAFLHER
                     MERGESTILVAEDRGQLLGFCQLYPTWCSVAAARVFILSDLFVDPDARRGGTGRALMR
                     AARDFGRQAGAARLDLQTAHANTKAQALYESEGWTLETVYRTYSLALDGGA"
     gene            4247..4876
                     /locus_tag="VIN75_18565"
     CDS             4247..4876
                     /locus_tag="VIN75_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY8976224.1"
                     /translation="MTLATWITFFLACWAISLSPGPGAIAAMTAGLNQGFKRGYFTVL
                     GLVLGIWTQMIIVVIGLGAIIKASETAFTAMKFLGAAYLIWVGISQWRSSDKPMVAAS
                     TDAPVLTRKQLIVRGWMINATNPKGTIFMLAVVPNFVDLAHPLLPQYLIIMMSLSFTD
                     LVVMAGYVALAAKVLHALREPHHLRVMNRVFGSLFVVAGTLLATFRRTA"
     gene            4889..5647
                     /locus_tag="VIN75_18570"
     CDS             4889..5647
                     /locus_tag="VIN75_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8976225.1"
                     /translation="MRADTLVILTGASRGLGQGVAAQYLAQGAFVLGLARHHAAELKA
                     GDTLEQWPIDLADPLPVAARLGAWLAEFEARAGGKPPARVRLIHNAAMLSEPGDIAGS
                     DPADLAQSLRIGLEAPIALTAAFLRATAHWSDTDRRVLFVSSGLGRRAMAGSAAYCAQ
                     KAGLDHFARALALEEEARPGGARVASVAPGIIDTDMQKQLRGADPQRFPERARFDEFH
                     RSGALDTPATAAGKLIALLERDDYGANPVADVRG"
BASE COUNT          733 a         2107 c         2043 g          844 t
ORIGIN      
        1 ccacgaagcc ggtggcgagc gcgtcggccc agtcgccgcg cccgttcgcc tcggcgcgcg
       61 cggcggcggc ggcccagtcg tagggcgtcg cgcgcagctg cacgtgccac gcgccgcggc
      121 gatcgcgctc cagcagggcc cagcgggcgt ccggcgagcc ggtctcgacg acgtgcgcga
      181 acgggtggtc gtcctcgtac gcctgcaggc cgacgctgcc cgggttgacc agcagcgggc
      241 cgccgggcac cgccatcgcg cgcggcacgt gcgtgtggcc gcacagcagc agcgcggacg
      301 ccaggcccgc gcgggtgtca cccggcgggt gctcgccggc gcgcgcggcc agttccaccg
      361 ccgtggccgc acgcatgccg cgctggccgt cgacgccgaa acccgggatc accgtctcca
      421 tgaagtagac caggtcgctg gccggcgtgc cgtggcacag cagcacgtcg tcggccagcc
      481 agtgcgtggg cggcagcgcg gccaaccacg cgcgctcact cgcgcccagc gcgcgcgccg
      541 cgtacgcgtc ggacgccccc atgcgcgcga cgtcgggcgc gagcacctgg cgctcgtggt
      601 tgccggcgat ggtgcgccag cgatccggct cggcggcggc gcgcgccatc agccattgca
      661 cggtttcgcc gggccacagc gggccggacg cgatgtcgcc gagattgacg accatcgtcg
      721 gcgatgcgcg ctcgatctcg gcccacacgg cctcgagggc gggcaggttg ccgtggatgt
      781 cggagacggc ggcgatgcgg aaggtctgca cggtggagct cgggcggccg gggtgcggcg
      841 gacgtcccag ggtagcaccg ccgccctgcc ggcgcgcaat gggccgccgt cgacggcgcg
      901 tttccgcgcg ggccggccgc gcccgcgccc attcgtcggc agaatccgtc gatttgtcct
      961 tcggagggat gcctgagcgc accaacgcca ctagcattcg tggcgcgtgg cgggcgttgg
     1021 tgcccgccac cgcaccggac cgaccttttc cgcgcaggcc tcgatcatga ccctcgactc
     1081 ccactcccgt atcgccgccg tcctccacat cggcctgggc gccctgtcgc tgtgcgtgct
     1141 cctggtcatc ggcgccatgg ctgctgccgt cggggtgtac ggggagacct tcggcctgga
     1201 caaggaactc ggcgcgatga tcggcggcat cggcatgatc gtggtcatct cgttcgcgct
     1261 gttcgcggtg ctggagatcg tcggcgcggt gatgctgatg cgcggcaacg attcgggtcg
     1321 cgtcatcacg ctcgtgttca gcgtgctgca cctggtcaac atcccgatcg gcacggccat
     1381 cggcggctat tcgctgtggg cgctgctgcg caacgcgccc cagccgatgc cggcggcgac
     1441 ctcgatgccc tcgggacgcg ccggaccggt ctgacctgtc ccggcccgcg actgtcgcga
     1501 ggcgaccgtc cggcgctccg gcctgcggcg ccctcgcggc gacgtcaccg gccactccgg
     1561 cgagggatgg cggcgcggcc gaatatcgct acgatggtcc gctcgaccat ggcgcccgcc
     1621 gagccgcgcc gcatgcggtg ttttcggcgc cgatcggccc tcgcccttgt cccatcaacc
     1681 cgctggagac cgcatgcccg tcatcgatac ccacgtccgt gtcgccgcct ggctgcacat
     1741 cgtccaaggc gccctagccg tgtgcgtgat cggatgcatc ggcttgatcg tcggcttcac
     1801 cggcgtggcc gccggctcgg cggtgcatgg ccgggacgcc accatcgtgg cgtggctggc
     1861 cagcctcggt gcggtgctgt tcgccgtcat cctcgccttc ccgctgctgg agatcgtcgg
     1921 cggcgtgatg ctgctgggcg gcagcgtggc cggtcgaatc atcacgctca tcttcagcgt
     1981 gctgggcctg atcagcattc ccatcggcac ggcgatcggc atctactcgc tgtgggcgct
     2041 gctgcgcgag gcgcccgcgg cgccggctga cgcgggcgtg gtgcagcccg gcatgcggtc
     2101 gacctgacgc cggcgccaag ccacgactgg ccactccgcg attgcgtcac gccatgaccg
     2161 agtcacgcca ccactgagtc accccacgac tgccccggcc acgtccggcc gggcaccgcg
     2221 cagtgaagtg cggcctcgcg ggccggcgat cacagtccgc gcaacacctc gatcgcctcc
     2281 tcgatccgct ccaccgcgtg gatcgtcagc ccctcgatcg gcttcttggg cgcattggcc
     2341 ttgggcacga tggccacgga gaaacccagc ttggcggcct ccttcagccg gtcctggccg
     2401 cgcggcgcgg gccgtacttc cccggccagg cccacctcgc cgaacgccag gaagccgcgc
     2461 ggcaacgcgc ggttgcggat cgagctctgg atggccagca gcaccgccag gtcggctgcc
     2521 ggctcgctga tgcgcacgcc gccgacggcg ttgacgaaga cgtcctggtc gccggtggac
     2581 acgcccgcat gccggtgcag caccgccagc agcatcgcca ggcggtcgcg ctccaggccg
     2641 accgacagcc gccgcgggct cgggccgccc gcgtcgacca gcgcctggat ctcgacgagc
     2701 atcggccgcg tgccttccag cgtgaccagc acgcagctgc ccggcaccgg ctcgccgtgc
     2761 gtggacagga agatggcgct cgggttggcc acgcccttca ggccgcgctc ggtcatcgcg
     2821 aacacgccga tctcgttgac ggcgccgaaa cggttcttga acgcgcgcac gaggcggaag
     2881 ctggagtgcg tgtcgccctc gaagtacagc accgtgtcga cgatgtgctc cagcacgcgt
     2941 gggcccgcga ggctgccgtc cttggtgacg tggcccacca gcacgatggt ggtgccgccg
     3001 gacttggccg tgcgggtgag ctgcgccgag cactcgcgca cctgcgccac cgagccgggc
     3061 gccgacgtga gctgctcgga atagagcgtc tggatggagt cgatgacgca gaacgccggc
     3121 tgctcggact cgatcgtggc gaggatgcgc tcgaggttga tctcggccag cacgcgcacc
     3181 tgcgtgccgg gcaggtcgag ccggcgcgag cgcagcgcca cctgggcgct ggactcctcg
     3241 ccggtgacgt acagcacctt catctgcgtg gccagcgcgt cgacggcctg cagcagcagc
     3301 gtgctcttgc cgatgcccgg gtcgccgccg atcagcacca cgccgccggc cacgatgccg
     3361 ccgcccagca cgcgatcgag ctcctcgatg ccggtgggcg tgcgcgcgac gtcggtggcg
     3421 tcgatgtcgg ccagcgtggc cacgggctgg ctcttggcga gcgcctggaa gcggttcttc
     3481 gaggcgccgc ccggcggctc gccgatgcct tcctccagcg tgttccaggc cgagcagtgc
     3541 gggcagcggc ccagccactt ggggttcgtg ccgccgcagg cggagcaggt gaagacggtc
     3601 ttgggagcag ccatgcgctc attgtgacac cactgttcaa atccacaggt cggcatttcg
     3661 cctgatttcc ggcgcatggg ccgccgggtg gcgacacgca gcgccgttac gatcacgcca
     3721 tgaccgacct cacgctgcgc cccgcctcgc tggacgacct cgaccagctc gccccgctgt
     3781 tcgacgcgta ccgccgcttc tacgagcagc ccggcgacgt cgcgcgcgcc agcgcgttcc
     3841 tgcacgagcg catggagcgc ggcgagtcga cgatcctcgt cgccgaggat cggggccagc
     3901 tgctcggctt ctgccagctg tacccgacct ggtgctcggt ggcggcggcg cgcgtgttca
     3961 tcctgtcgga cctgttcgtg gacccggacg cgcgccgtgg cggcaccggc cgggcgctga
     4021 tgcgcgccgc gcgcgacttc ggccgccagg cgggcgccgc gcggctggac ctgcagacgg
     4081 cgcacgccaa cacgaaggcc caggcgctgt acgagtccga gggctggacg ctggagacgg
     4141 tctatcgcac ctacagcctg gcgctggacg gcggggcctg aaacccgctg gtcgtgcacg
     4201 tgcgccgaca ttcattcatc atgtcgggtc attcccgaaa gtcgtcatga ccctcgccac
     4261 ctggatcacc ttcttcctcg cctgctgggc gatcagcctg tcgccgggcc ccggcgccat
     4321 cgccgcgatg acggccgggc tcaaccaggg gttcaagcgc ggctacttca cggtgctggg
     4381 cctggtgctg ggcatctgga cgcagatgat catcgtggtg atcggcctgg gcgcgatcat
     4441 caaggcgtcg gagacggcat tcaccgcgat gaagttcctg ggcgcggcct acctgatctg
     4501 ggtcggcatc tcgcagtggc gctccagcga caagccgatg gtcgccgcgt cgaccgacgc
     4561 gccggtgctc acccgcaagc agctcatcgt gcgcggctgg atgatcaacg cgaccaaccc
     4621 caagggcacg atcttcatgc tcgcggtggt gccgaacttc gtcgacctgg cgcatccgct
     4681 gctgccgcag tacctgatca tcatgatgtc gctgtcgttc accgacctcg tggtgatggc
     4741 cggctacgtg gcgcttgcgg ccaaggtgct gcacgcgctg cgcgagccgc atcacctgcg
     4801 cgtgatgaac cgcgtcttcg gcagcctgtt cgtggtggcc ggcaccctgc tggcgacctt
     4861 ccgccgcacg gcctgaagga gggtgcgcat gcgtgccgac accctcgtca tcctcaccgg
     4921 cgcgtcgcgc ggcctgggcc agggcgtcgc ggcccagtac ctcgcgcagg gcgcgttcgt
     4981 gctcgggctg gcgcgccacc acgcggccga gctcaaggcc ggcgacacgc tggagcagtg
     5041 gccgatcgac ctggccgacc cgctgcccgt cgccgcgcgg ctgggcgctt ggctggccga
     5101 gttcgaggcg cgcgccggcg gcaagccgcc cgcccgggtg cgcctgatcc acaacgcggc
     5161 gatgctcagc gagccgggcg acatcgccgg cagcgacccc gccgacctgg cgcagagcct
     5221 gcgcatcggg ctggaggcgc ccatcgcgct gacggccgcg ttcctgcgcg ccacggcgca
     5281 ctggtcggac accgaccgcc gcgtgctgtt cgtgtcgtcc ggcctcggcc gccgggcgat
     5341 ggccggcagc gccgcctact gcgcgcagaa ggccgggctc gaccacttcg cgcgcgccct
     5401 ggcgctcgag gaggaggcgc ggcccggcgg cgcgcgcgtg gcgtcggtgg cgccgggcat
     5461 catcgacacc gacatgcaga agcagctgcg cggcgccgac ccgcagcgtt tcccggagcg
     5521 cgcgcgcttc gacgagttcc accgcagcgg cgcgctcgac acgccggcca cggcggcggg
     5581 caagctgatc gcgctgctgg agcgcgacga ctacggcgcg aacccggtgg cggacgtgcg
     5641 gggctgacgg gggcgcgcgc ctgccggccc gcgcggtcga ggcgcgcgcc tgcgggttcg
     5701 agcgccagcc cgcgagccga gccgcgc
//
LOCUS       DATCRO010000686         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_355829, whole genome shotgun sequence.
ACCESSION   DATCRO010000686 DATCRO010000000
VERSION     DATCRO010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355829"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIN75_18575"
     CDS             <1..390
                     /locus_tag="VIN75_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976226.1"
                     /translation="GYAPTSWLGLGENLPAQVARQWGQWCAAGGYATNAVRAGLADDF
                     HAQVRTPIVVLHAEDDDIANPATVGDLLRTLPASPRAALQVRPRDHGLRAIGHLDWFR
                     QSHQALWPLLRRAVLGDIAVQAPPARA"
     gene            complement(431..784)
                     /locus_tag="VIN75_18580"
     CDS             complement(431..784)
                     /locus_tag="VIN75_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEY8976227.1"
                     /translation="MNYVDGFVVAVPTADKETYRAYCQRMAAVFREHGALSVVDTWGD
                     DVPQGQLTSFPLAVKCAADETVAFGWTTWASRAARDEAWQKIMADPRMKTETNPMPFD
                     GKRMIYGGFEVISKA"
     gene            complement(886..1587)
                     /locus_tag="VIN75_18585"
     CDS             complement(886..1587)
                     /locus_tag="VIN75_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8976228.1"
                     /translation="MTGYRLSERLRESIEEEIATGKLLPGSRLDEVELATRFGVSRTP
                     IREALRLLLGEGLVETRPQRGAVVAQVTAQRLIEMFEVMAELEAMCARLAARRMSDAE
                     LAEVEAAHEACRGSAAARDADAYFYANERFHYAIYAASHNTFLFEQAAALQRKLRPYR
                     RLQLRVRNRPQRSFEEHQAILDALREGDADKAQQSIRSHVVVQGERFADLVASLGQMT
                     GQPAPDAPAEPARSA"
     gene            1784..>3495
                     /locus_tag="VIN75_18590"
     CDS             1784..>3495
                     /locus_tag="VIN75_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8976229.1"
                     /translation="MIHLKTFGVATVLLATWNATSSTPLPTRPAPTPAVQQRAAGIVD
                     RFEVVSTVDAWGGATPAGAAGPYTVVTGIVHGRLLPTHPDNAGIVDLANAPRDADGYV
                     DYTTDAVILRPKSASTARRVMFYDVANRGSKPALSAFIGASALVKGAAPGENFPSLLR
                     AGYTVVWSGWQSNVAQSGAGATAAVGARFPVAKRPDGSPITGLSREEYIPEMVADGTY
                     RFALTYAPASLTDFSEVRFTARQSWIGADGKQSYGAPSAQVVNWHYVPNDNGTAFVEF
                     TPPATVPAADGSAVAPGPGTIYSFVFRAKDPVVSGIGFAAVRDLVGFLRYADKDEQGN
                     ANPVADLKAAACAAGTACPAHPQGNFDVAIAAGTSQSGRFLRDFLYQGFNKDGQGAKV
                     FDGLLPMIAGARRTWVNERFAQPGRVSREHEDHWMPGDQFPFAYGVATDPVGGRTDGL
                     LKSCQASATCPKIMQVDGSFEWWAGRASLVVTDGAGRDVALPDNVRYYLVAGTQHGDG
                     GGVGSGLAYQAGAATRCLLAPSPVQESPVERALVPALEAWIVKGKRPPASRYPTVAGG
                     GLATP"
BASE COUNT          482 a         1332 c         1203 g          478 t
ORIGIN      
        1 ggctatgcgc cgacctcgtg gctgggcctg ggcgagaacc tgccggcgca ggtcgcgcgc
       61 cagtggggcc agtggtgcgc ggccggcggc tacgccacca acgccgtgcg cgccggcctc
      121 gccgacgatt tccatgccca ggtgcgcacg cccatcgtgg ttctgcatgc ggaggacgac
      181 gacatcgcca acccggcgac ggtgggcgac ctgctgcgca cgctgcccgc gtcgccgcgc
      241 gccgcgctgc aggtgcgccc gcgcgaccac ggcctgcgcg cgatcgggca cctcgactgg
      301 ttccgccagt cgcaccaggc gctgtggccg ctgctgcggc gggcggtgct gggcgacatc
      361 gcggtgcagg cgccgccggc gcgcgcgtag cgactcgcga gggcgtgacc cgccatgtag
      421 ccgacgccga tcacgccttg gagatcacct cgaagccgcc gtagatcatc cgcttgccgt
      481 cgaacggcat cgggttggtc tcggtcttca tgcgcgggtc ggccatgatc ttctgccagg
      541 cctcgtcgcg cgcggcgcgc gaagcccagg tcgtccagcc gaacgcgacg gtctcgtcgg
      601 ccgcgcactt gacggccagc gggaaggacg tcagctggcc ctgcggcacg tcgtcgcccc
      661 aggtgtcgac cacgctcagc gcgccgtgct cgcggaacac cgcggccatg cgctggcagt
      721 acgcccggta ggtctccttg tcggcggtgg gcacggccac cacgaatcca tcgacgtagt
      781 tcatgcaggt ctcctggaca gcggggaagg ccacgccggc ggcggcgtgg gtttcaagcg
      841 tacgacgaat cgccgcggcc ggaatcgaca ggccggggcc cgggatcagg cgctgcgggc
      901 aggctcggcg ggcgcgtcgg gcgccggctg gccggtcatc tggccgaggc tggccaccag
      961 gtcggcgaag cgctcgccct gcacgaccac gtgcgagcgg atgctctgct gggccttgtc
     1021 ggcatcgccc tcgcgcagcg cgtcgaggat ggcctggtgc tcctcgaacg agcgctgcgg
     1081 ccggttgcgc acgcgcagct gcagccggcg gtacgggcgc agcttgcgct gcagcgcggc
     1141 cgcctgctcg aacaggaagg tgttgtgcga cgccgcgtag atggcgtagt ggaagcgctc
     1201 gttggcgtag aagtaggcgt cggcgtcgcg cgcggcggcc gagccgcggc aggcctcgtg
     1261 ggcggcctcg acctcggcca gctcggcgtc ggacatgcgc cgcgccgcga gccgcgcgca
     1321 catcgcctcc agctcggcca tgacctcgaa catctcgatc agccgctgcg ccgtgacctg
     1381 tgccaccacc gcgccgcgct gcggccgcgt ctcgaccagc ccctcgccca gcagcagccg
     1441 cagcgcctcg cggatcgggg tgcgcgacac gccgaaacgc gtggccagct cgacctcgtc
     1501 cagccgcgag cccggcagga gcttgcccgt ggcgatctct tcctcgatgg actcgcgcag
     1561 gcgctcggaa agccgataac cggtcatgcg gcggattcta ggcgcacgcc gccgtgaatt
     1621 cctgccggat tcgcgtgcaa acaggcggcc gcacggataa cggatcgcca aagcggaatt
     1681 cattcatacc cgttctggat ccaacggtcg aagattcctc catcgcgagg tctaggcctc
     1741 ccgactgtcc gatgacagca ggaatgcgcc aatccaagac accgtgatcc acctcaagac
     1801 cttcggcgtc gccaccgttc tgctggcgac ctggaacgcc accagctcca cccccctgcc
     1861 cacgcgcccg gctcccacgc cggcggtgca gcagcgcgcg gccggcatcg tggatcgttt
     1921 cgaggtggtc tccaccgtcg acgcctgggg cggcgcgacg cccgccggcg ccgccggccc
     1981 gtacacggtc gtcaccggca tcgtccacgg ccgcctgctg cccacgcacc ccgacaacgc
     2041 cggcatcgtc gacctggcca atgccccgcg cgacgccgac ggctacgtcg actacacgac
     2101 cgacgccgtc atcctgcgcc cgaagtcggc ctcgacggcg cgccgcgtga tgttctacga
     2161 cgtcgccaac cgcggctcca agccggcgct gtcggccttc attggcgcga gcgcgctggt
     2221 caagggcgcc gcgcccggcg agaacttccc gtcgctcctg cgtgcgggct acaccgtggt
     2281 gtggagcggc tggcagtcca acgtcgcgca gtccggcgcc ggcgccaccg ccgccgtcgg
     2341 cgcccgcttc cccgtggcca agcgcccgga cggctcgccg atcaccggcc tgagccgcga
     2401 ggaatacatc ccggagatgg tggccgacgg cacgtaccgc ttcgcgctga cctacgcgcc
     2461 ggcctcgctg accgacttct ccgaagtgcg cttcaccgcc cgccagagct ggatcggcgc
     2521 cgacggcaag cagagctacg gcgcgccgtc cgcgcaggtc gtcaactggc actacgtccc
     2581 caacgacaac ggcaccgcct tcgtcgagtt cacgccgccc gccaccgtgc cggccgccga
     2641 cggctcggcc gtcgcgccgg gaccgggcac gatctacagc ttcgtcttcc gtgccaagga
     2701 tccggtggtc tcgggcatcg gcttcgcggc cgtgcgcgac ctggtgggct tcctgcgcta
     2761 cgccgacaag gacgaacagg gcaacgcgaa cccggtcgcc gacctgaagg ctgccgcctg
     2821 cgccgccggc accgcgtgcc cggcgcaccc gcagggcaac ttcgacgtgg cgatcgccgc
     2881 gggcacctcg cagtcgggcc gcttcctgcg cgacttcctc taccagggct tcaacaagga
     2941 cggccagggc gccaaggtgt tcgacggcct gctgccgatg atcgccggcg cccgtcgcac
     3001 ctgggtcaac gaacgcttcg cccagccggg acgcgtgtcg cgcgagcacg aggaccactg
     3061 gatgcccggc gaccagttcc cgttcgccta cggcgtggcg accgaccccg tcggcggccg
     3121 caccgacggc ctgctcaaga gctgccaggc cagcgccacc tgcccgaaga tcatgcaggt
     3181 cgacggcagc ttcgaatggt gggccggccg cgcctcgctg gtggtcaccg acggcgcggg
     3241 ccgcgacgtc gcgctgcccg acaacgtgcg ctactacctc gtggccggca cccagcacgg
     3301 cgacggcggc ggcgtcggct ccggcctcgc ctaccaggcc ggcgccgcca cgcgctgcct
     3361 gctcgccccg agccccgtgc aggaatcgcc cgtcgagcgc gcgctggtcc cggccctgga
     3421 ggcatggatc gtcaagggca agcgcccgcc ggcgtcgcgc tacccgacgg tcgccggtgg
     3481 cggcctcgcc acgcc
//
LOCUS       DATCRO010000687         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_761042, whole genome shotgun sequence.
ACCESSION   DATCRO010000687 DATCRO010000000
VERSION     DATCRO010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761042"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="aceE"
                     /locus_tag="VIN75_18595"
     CDS             complement(<1..1083)
                     /gene="aceE"
                     /locus_tag="VIN75_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747281.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8976230.1"
                     /translation="MSAIPNSLPGAAANDADAQETREWTDALAAVIAVEGRERGHFLI
                     EQLLDHARQQGIDLPFSANTAYVNTIAPEDEAQFPGNVDIEERLRAYMRWNAMAMVVK
                     ANRLHPADGGDLGGHLSSFASLATMLGCGFNHFWHGADGEHGGDLLYIQGHSSPGIYA
                     RAYLEGRITEEQLLNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLMAIYQARF
                     LKYLHARGIADTSKRKVWVFCGDGEMDEPESLGAIGLAAREKLDNLIFVVNCNLQRLD
                     GPVRGNGKIIQELESEFRGAGWNVIKLLWGSYWDPLLARDKDGALKKLMMETLDGDYQ
                     AFKSNDGAFVRKNFFGRDPKTLEMVAK"
     gene            1336..>2847
                     /locus_tag="VIN75_18600"
     CDS             1336..>2847
                     /locus_tag="VIN75_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8976231.1"
                     /translation="MQPVPESGSTPSPSWTQRVFGRWWRSQSPSRQDRFATLGPLVSV
                     LLFLAAIVAAFWTLRSEEIERATDAVRRDTEVAQQQIRSRLSEDQELMVRLTRDIVTH
                     DVDTDSFFTQTASFAPARPELTQVIWLNADRTPRATFLGSIFRGAASLRNSAEDPSLP
                     HQGANTASEAAFTAARNTHVPQFSKPFIDVDGTAVFQVQIPLISHTAFTGVLVAEYSI
                     EALLRHAVPEEVTKRRAIEVLDEHEELLASTITTMPGRPSAVPSIAFEAPLAPASNGL
                     ILRGLGYRTSIGLVSNSLFWMVLALSGLTVWMLLGTWRHVRRRAQIQSALVHETNFRR
                     AMENSMLTGMRAMDMEGRITYVNPAFCAMTGFSDNELVGKLPPFPHWPHDRIEENSRL
                     LQQELQGRSPAGGIEVKIMRKDTSVFDARMYVSPLIDAKGVQTGWMTSVTNITEAKRV
                     RDQLSASHERFTTVLEGLDAAVSVLSVQQGQLLFANRSYRLWFGSDAHIHAVLA"
BASE COUNT          433 a          975 c          969 g          470 t
ORIGIN      
        1 cttggcgacc atctccagcg tcttcgggtc gcggccgaag aagttcttgc gcacgaacgc
       61 gccgtcgttg gacttgaacg cctggtagtc gccgtccagc gtctccatca tcagcttctt
      121 gagcgcgccg tccttgtcgc gcgcgagcag cggatcccag tagctgcccc acagcagctt
      181 gatgacgttc cagccggcgc cgcggaactc gctctcgagc tcctggatga tcttgccgtt
      241 gccgcgcacc gggccatcga ggcgctgcag gttgcagttg acgacgaaga tcaggttgtc
      301 gagcttctcg cgcgcggcca ggccgatggc gcccagcgac tcgggctcgt ccatctcgcc
      361 gtcgccgcag aacacccaga ccttgcgctt gctggtgtcg gcgatgccgc gggcgtgcag
      421 gtacttgagg aagcgcgcct ggtagatggc catcagcggg cccaggccca tcgacaccgt
      481 cgggaactgc cagaagttgg gcatcagctt cgggtgcggg tagctcgaga gccccttgcc
      541 gtcgacctcc tggcggaagt tcagcagctg ttcctcggtg atgcggccct cgaggtaggc
      601 gcgggcgtag atgccgggcg acgagtggcc ctggatgtag agcaggtcgc cgccgtgctc
      661 gccgtcggcg ccgtgccaga agtggttgaa gccgcagccc agcatcgtgg ccagcgaggc
      721 gaacgacgac aggtggccgc cgaggtcgcc gccgtcggcc gggtgcaggc ggttggcctt
      781 gacgaccatc gccatcgcgt tccagcgcat gtaggcgcgc aggcgctcct cgatgtcgac
      841 gttgcccggg aactgcgcct cgtcctccgg cgcgatcgtg ttgacgtacg ccgtgttggc
      901 cgagaacggc aggtcgatgc cctgctggcg cgcgtgatcg agcagctgct cgatcaggaa
      961 atggccgcgt tcacggcctt cgacggcgat gaccgcggcc agcgcgtcgg tccattcgcg
     1021 ggtttcctgc gcatcggcgt cgttggcggc ggcgccggga agggaattgg gaatcgcgga
     1081 catgcctgtc tcctgggctt tgtagagcgg tcgatctaaa ccgggcggag tgtcgcacaa
     1141 gaattcaccg atttcaaatg gcgacagttc atttcatgat atgcaatttc gctgcgatgc
     1201 agcacgaaga tttccagcga agcgcgcaag cgcccggccg ccgcgcccgg ccgctccgga
     1261 gcaaggccgg cccccggcgg cgggtgccag cggtactccg ccgccggggg ccgacactga
     1321 ttcgataatg cccggatgca acctgttccc gagtccggct cgaccccgtc accgtcatgg
     1381 acgcagcgcg tcttcggccg ctggtggcgc agccagtcgc cgtcgcgcca ggaccgcttc
     1441 gccacgctcg gtccgctggt ctcggtgctg ctgttcctgg ccgcgatcgt cgcggcgttc
     1501 tggacgctgc gcagcgagga gatcgagcgc gccaccgacg ccgtgcggcg cgacaccgag
     1561 gtcgcgcagc agcagatccg ctcgcgcctg tccgaagacc aggaactgat ggtgcggctg
     1621 acgcgcgaca tcgtcacgca cgacgtcgac accgacagct tcttcacgca gacggccagc
     1681 ttcgcgccgg cgcggcccga gctgacgcag gtcatctggc tcaacgccga ccgcacgccg
     1741 cgcgcgacct tcctcggctc gatcttccgc ggcgcggcca gcctgcgcaa cagcgcggag
     1801 gacccgtcgc tgccgcacca gggcgcgaac acggcgtcgg aggcggcctt cacggcggcg
     1861 cgcaacacgc acgtgccgca gttctccaag cccttcatcg acgtcgacgg caccgcggtc
     1921 ttccaggtgc agatcccgct gatctcgcac accgcgttca cgggcgtgct ggtggccgag
     1981 tactcgatcg aggcgctgct gcgccacgcc gtgcccgagg aggtgaccaa gcggcgcgcg
     2041 atcgaggtgc tcgacgagca cgaggaactg ctggccagca cgatcacgac gatgccgggc
     2101 cgtccgtcgg ccgtgccgtc gatcgcgttc gaggcgccgc tggcgccggc gtccaacggg
     2161 ctgatcctgc gcgggctggg ctaccgcacg tcgatcggcc tggtctccaa ctcgctgttc
     2221 tggatggtgc tggcgctgtc gggcctgacg gtgtggatgc tgctgggcac ctggcgccac
     2281 gtgcgccgcc gcgcgcagat ccagagcgca ctggtgcacg agaccaactt ccggcgcgcg
     2341 atggagaact ccatgctcac gggcatgcgc gcgatggaca tggagggccg catcacctac
     2401 gtcaaccccg cgttctgcgc gatgacgggc ttctctgaca acgagctcgt cggcaagctg
     2461 ccgcccttcc cgcactggcc gcacgatcgc atcgaggaga actcgcgcct gctgcaacag
     2521 gagctgcagg gccgcagccc ggccggcggc atcgaggtca agatcatgcg caaggacacc
     2581 tcggtgttcg acgcgcgcat gtacgtctcg ccgctgatcg atgccaaggg cgtgcagacc
     2641 ggctggatga cctcggtcac caacatcacc gaggccaagc gcgtgcgcga ccagctgtcg
     2701 gcctcgcacg agcgcttcac gacggtgctc gaaggcctgg acgcggcggt gtcggtgctg
     2761 tcggtgcagc agggccagct gctgttcgcc aatcgttcgt accgcctctg gttcggctcc
     2821 gacgcccaca tccatgcagt gctggcc
//
LOCUS       DATCRO010000688        13291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1110168, whole genome shotgun sequence.
ACCESSION   DATCRO010000688 DATCRO010000000
VERSION     DATCRO010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110168"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..959)
                     /locus_tag="VIN75_18605"
     CDS             complement(15..959)
                     /locus_tag="VIN75_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY8976232.1"
                     /translation="MIELDVLTAGEALVLLAAESPGALEHVERFRRFSAGAELNVAIG
                     LSRLGLRVGYVSRVGNDAFGRFLLAQMEREGLSLGLVATDRTRSTGFMLKSLAVDGAD
                     PLIDYYRRGAAASAMDATELAAAGPIAARHVHLTGILPALSAGCRELAFAMAATARAQ
                     GRRVSFDPNLRPRLWPSQEEMAMTINGLAARSDVVLPGLSEGRLLTGKDSPEDIAAFY
                     LDLGASAVAVKLGAQGAFCADRSGLRTFVPGVPVRQVVDTVGAGDGFAVGVISGLLEG
                     MDLAAATRRGNAIGARMVQFPGDSDGLPTRQELDGLAA"
     gene            complement(956..2488)
                     /locus_tag="VIN75_18610"
     CDS             complement(956..2488)
                     /locus_tag="VIN75_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEY8976233.1"
                     /translation="MSTESASSVLLRLHPADDVAVARDAVAAGSLLAGPEGVLRAADD
                     VPAGHKIALRAVGQGAPVRKYGQVIGVATVAIAAGQHVHVHNLDMGESHPAHAVGRAY
                     VPTVPVFEPASFEGFVRSDGSVGTRNFVGVISSVNCSATVCKAIEKAFAGDAMAPWPG
                     VDGVVAITHGGGCGMSGRGEGIALLRRTLRGYATHPNFAGVLVIGLGCEVNQVGALVE
                     PLVASGDRIRTMTIQEAGGTREAIDEGCAIVRELLAVANAARREKVPASRLMVGLQCG
                     GSDGYSGISANPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTARAARPEIAERLMQ
                     RLRWWEDYTGKNGGDMNNNPSPGNKAGGITTILEKSLGAVAKGGTSGLMDVVEYAEPV
                     GTHGLVFMDTPGYDPVSATGQVAGGANLICFTTGRGSTYGCKPTPSIKLATNSQMYGR
                     MRMDMDFNAGEIIDGRLGVHEAGAALFELMLATASGRRTLSEQHDLGNNEFVPWQLGA
                     VM"
     gene            complement(2478..2807)
                     /locus_tag="VIN75_18615"
     CDS             complement(2478..2807)
                     /locus_tag="VIN75_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY8976234.1"
                     /translation="MKRMGMVIGVAPERIAEYKRLHAAVWPGVLERIAASGIRNYSIY
                     LREPENLLFACWEYHGTDLAADMAAIAADPETQRWWTFTDPCQRPLDSRAPGEHWAAM
                     EEVFHVD"
     gene            complement(2804..3967)
                     /locus_tag="VIN75_18620"
     CDS             complement(2804..3967)
                     /locus_tag="VIN75_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY8976235.1"
                     /translation="MSTGQPILQFGTSRFLQAHVDLLVSEAMARGDALGGITVVQTTD
                     DPRSTARVAALASGRGHPVRIRGLANGRPVDEQRHCHAVLAALRTATDWPAVRREFAS
                     DTLQVVVSNTGDAGWQLDDTDGPQALAPQAAAPRAFPAKLLVALHDRWQARPDAALTL
                     LPCELVARNGERLRDIVVGLAMQWQLDAAFQAWLRERPIWANSLVDRIVPEAIEPVGA
                     IAEPYALWAIERQPGLVPPCRHADIVLTEDLGPFERLKLWLLNLGHTALAELWLRDGQ
                     PHGRTVLDAMRDESTRLALERLWGEEVLPVFEALGEGRAARAYLAGLRERLLNPWLAH
                     RLADIAANHAQKKERRLAPVVAAAQRCAPALAQPWLRGALASTSRLETKESST"
     gene            complement(3970..4980)
                     /locus_tag="VIN75_18625"
     CDS             complement(3970..4980)
                     /locus_tag="VIN75_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8976236.1"
                     /translation="MLTVLCETPGKLVAEQRPRPPRQPGEVLLRVRRVGVCGTDLHIF
                     TGKQPYLAYPRVMGHELSGIVEEADPASGLAPGDAVYVMPYLSCGRCVACRQGKTNCC
                     TSIQVLGVHRDGAMTEYLAVPAPFVHKAEGITLDEAAMLEFLAIGAHAVARGDVASGQ
                     RVLVAGAGPIGLAVMLFAARRGARVVALDTRRDRLDVACAHLGIEAAVEVGPDDAREL
                     ARLSDGEHFDVVFDATGNPQAMERGFAFIAHGGRYVLVSIVQGDISFADPEFHKREAT
                     LLSSRNATTADFETVLAAMRAGDVPVAALATHRMQLADLPRDFPALLEPGQHVIKAIV
                     EC"
     gene            complement(4974..5828)
                     /locus_tag="VIN75_18630"
     CDS             complement(4974..5828)
                     /locus_tag="VIN75_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8976237.1"
                     /translation="MRIDAHQHFWRLAERGGAWPPPTLAAIHRDFEPGDLAPLLQAGG
                     IDGTVLVQSLPTVAETRRLLALARTTRFVHGVVGWVDMKAPDAVDTIAELARDPLLKG
                     LRPMLQDLEEDDWIADPATAPAAQAMARHGLVFDALVLPRHLPALLTFAARHPQLLVV
                     VDHAAKPPIARGQMLPWQDDLARLAALPHVHCKVSGLLTEAGDRARIGDLLPYVQALW
                     SLFGPSRLLWGSDWPVLQLAGDYAGWLAMTTALLAAVTPPASAQEAAAILGDNAVTLY
                     RLQGSRPC"
     gene            complement(5825..6847)
                     /locus_tag="VIN75_18635"
     CDS             complement(5825..6847)
                     /locus_tag="VIN75_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8976238.1"
                     /translation="MKAADRRTLAGGRLRLTALGLGAAALAGMYAPVTEQEAADTLAA
                     ARDAGLRYIDTAPYYGYTLGEHRVGAFLREQPRHEFTISTKVGRLMRPDASVRPGSDG
                     WARPLPFRPAFDYGRDAILRSVEDSLQRLGLDRVDLLLVHDIGRVTHGDAHEAHWDAL
                     TRGGGFRALEELRRDGQVAAIGLGVNEWQVMREAMQECELDCSLLAGRYTLLEQGSLP
                     FLDECARRHHAIVVGGPFNSGLLAGQAKFDYTDAPPELLRRAAALRSTCADFDVPLEA
                     AALQFPLGHPAVVSVLSGMRSPAQVRQNVAWFERAIPAGLWDALRDQGLLEGAAPCPQ
                     DGGARP"
     gene            complement(6852..7796)
                     /locus_tag="VIN75_18640"
     CDS             complement(6852..7796)
                     /locus_tag="VIN75_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006757164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976239.1"
                     /translation="MKRLFHAVLASALLLAGAAATAADKEIAVIVKTANSDFWQNVRK
                     GATQAASELKGYTASFQGAASDTDLAGQVALVENAVNRKVAAIVLAPSDPEALIPAIK
                     KAWEAHIPVILIDSAAADSGKDYYQSFLATDNNKAGELCAQALIERIGTTGKIAIMSY
                     TAGSGSEIGRVGGFRKYIEAHSKLQIVGPFYSNSQMGTALNQTTDVLAANPDLKGIFG
                     ANEPTAIGMGRALVQAGKAGKVVAIGFDGNADLQGFVKDGTISSIAVQSAYQMGNLGV
                     KTAVDVLNKKPVPKFRDTGVVMVTKTNIDTPAAKNVLY"
     gene            complement(7867..8850)
                     /locus_tag="VIN75_18645"
     CDS             complement(7867..8850)
                     /locus_tag="VIN75_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976240.1"
                     /translation="MNERQKDLVQKFAALGGLFVLVTVFSLASPAFFSVGNGMTVALQ
                     VTSIAYLGIGATCVIITGGIDLSVGSILALSGVVAALAVRGGVNVPLAMLLGILTGVG
                     CGLVNGLCVTVMRLPPFIATLAMMLVARGVALQITDAKAVGGLGEQFAALGNGSLWRI
                     VRIGADGFPDVVFPGIPYPVLLMIALALAIGLMLGRSRLGRHLYAVGSNAEAARLSGI
                     DVRRVTLLAYVLSGALAGLTGCVLMSRLVTAQPSEGVMYELDAIAAAVIGGTSLTGGI
                     GTVSGTLIGAFVIGILRNGLNMTGVSAFVQQIIIGLVILVTVWIDQLRNRR"
     gene            complement(8847..10397)
                     /locus_tag="VIN75_18650"
     CDS             complement(8847..10397)
                     /locus_tag="VIN75_18650"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY8976241.1"
                     /translation="MTSTNDATPALLCLSGVTKRFFNVVALEGVSFDVRGGEVHAVCG
                     ENGAGKSTLMKVMSGVYAPDEGTLKLDGEPRRFASPREAQEQGIAMIHQELNLVPHLS
                     VAENIYLGREPLRGWLVDRRKLRADAQACLDQLGVSISPDELVAGLSVAQQQMVEIAK
                     ALSMRARLLIMDEPTSSLTDAETTQLFRVIHELKRAGSGIVYISHRLDELEGLVDRVT
                     VLRDGRYIATKTFAETSVAEIVALMVGRTLDKQFPPARRQPTAETVLRVRGLRRQGVF
                     GPVDFELRRGEILGFAGLMGAGRTEVARAVFGADPIDGGHIELGGRSLTVRSPIDAIR
                     EGIAYLSEDRKAHGLAVKMSVATNITLANTAAVTDWRGLIRAHEEIAIARRYIEQLGI
                     RTPSPDQVARLLSGGNQQKVVIAKWLFRDSRVLFFDEPTRGIDVGAKVAIYELLDQLA
                     CAGIGVVMISSELPEILGMTDRIAVFHGGRITTVLKTRATSQEEIMHFASTCLPRAAL
                     PQQESLVP"
     gene            10610..11356
                     /locus_tag="VIN75_18655"
     CDS             10610..11356
                     /locus_tag="VIN75_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY8976242.1"
                     /translation="MTATPKPVDTRRLYQQVADRIRTVIREGQYEVGSRLPPERELAL
                     QLGVSRPSLREALIALEIDGRIEIRMGSGVYVCAPPVAAERHTPALGESPTELMQARA
                     TLEGAVVALAAARVTRAGLDRVADSLAAMKREHARGRMPVEADRRFHLGIAELAGNTV
                     LVRMVGELFDGRHSPISSRMSGHAETVQTWTVALAEHERIFRALEARDPQAATAAMFT
                     HLRASEERWVSEPAASIESAPAFPTIKERP"
     gene            11353..12084
                     /locus_tag="VIN75_18660"
     CDS             11353..12084
                     /locus_tag="VIN75_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8976243.1"
                     /translation="MRLAGKTAFITAAGNGIGRAAAIAFAAEGAHVIATDVDGAALAS
                     LDHPALRTHVLDVRDGDAVRRVAGASGTVDVLFNCAGIVHHGTLLECDDEAWARSMDI
                     NVGSMHRVTRALLPGMLAAGRGSIINMASMASSIKGFPNRCVYGASKAAVIGLTKAIA
                     ADHVRQGIRCNAVCPGTVDTPSLRGRIAAAADPAQAERDFIARQPMGRLAVVDDITPL
                     LVYLASDESRFVTGQALSVDGGVTI"
     gene            12097..12942
                     /locus_tag="VIN75_18665"
     CDS             12097..12942
                     /locus_tag="VIN75_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8976244.1"
                     /translation="MKLLRHGPAGLEKPGMLDSQGQVRDLAGIVRDIAGATLTDAGLA
                     SLRMLDPGTLPLVAPGTRLGPCVGAVGKFVCIGLNYADHAAESGMPIPAEPVVFGKWT
                     SAIAGPDDAIEIPRGSTKTDWEVELGVVIGEGGRHIAQADALRHVAGYCVVNDISERE
                     YQLERGGQWDKGKGCDTFGPIGPWLVTRDEVPDPQGLGLWLDLNGRRMQDGNTRTMVF
                     GVAHIVSYLSRFMSLQPGDVISTGTPPGVGLGQKPPLYLRAGDVMRLGIEGLGQQTQR
                     VIDAA"
     gene            complement(13051..>13291)
                     /locus_tag="VIN75_18670"
     CDS             complement(13051..>13291)
                     /locus_tag="VIN75_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEY8976245.1"
                     /translation="YQLELRRTGYRANDAYTAYLEMGAPQQLDDAQLARLQSLTLDRP
                     ESVRTVTIGKNGLLDFTVPMHSNDIALLTLQPVKP"
BASE COUNT         1973 a         4604 c         4777 g         1937 t
ORIGIN      
        1 gccggggccc gccgtcaggc ggccaggccg tccagctcct gccgtgtcgg caacccgtcg
       61 ctgtcgcccg ggaattgcac catccgggcg ccgatcgcgt tgccgcgcct cgtggccgcc
      121 gccaggtcca tgccctccag caggccgctg atcacgccca ccgcaaagcc gtcgccggcg
      181 ccgacggtgt cgaccacctg ccgcacgggg acgcccggca cgaaagtgcg caggccgctg
      241 cgatccgcac agaacgcacc ctgggcaccg agcttgacgg cgaccgcgct ggcacccagg
      301 tcgaggtaga acgcggcaat gtcttcgggg ctgtcctttc ccgtcagcag ccggccttcg
      361 gacaggcccg gcagcaccac gtcggaccgg gctgcgagcc cgttgatcgt catggccatt
      421 tcttcctgcg acggccagag ccgggggcgc aggttgggat cgaacgacac gcggcgacct
      481 tgcgcacgcg ctgtcgcggc catggcgaac gccagttcgc ggcaaccggc tgagagcgcc
      541 gggaggattc cggtcaggtg gacatggcgg gcggcgatgg gcccggccgc ggccagttcg
      601 gtcgcgtcca tcgcactggc cgcggcgccg cggcggtagt agtcgatgag cgggtcggcc
      661 ccgtcgaccg cgagggactt cagcatgaag ccggtcgatc gggtgcgatc ggtggcgacg
      721 agtccgagcg aaagcccttc gcgttccatc tgcgccagca ggaacctccc gaaggcatcg
      781 ttgccgacgc ggctcacgta gcccacgcgc aggccgagcc gcgacagtcc gatcgccacg
      841 ttgagttcgg cgcctgcgct gaaacggcgg aagcgctcga cgtgctcgag cgcgccgggg
      901 ctctcggcgg cgagcagcac cagcgcttcg ccggcggtca ggacatccag ctcgatcaca
      961 tgaccgcccc gagctgccac ggcacgaact cgttgttgcc caggtcgtgc tgctcgctga
     1021 gcgtccgccg tcccgacgcc gtggccagca tcagctcgaa cagtgctgcc ccggcctcgt
     1081 gcacgccgag gcggccgtcg atgatctctc ccgcgttgaa atccatgtcc atgcgcatgc
     1141 ggccgtacat ctggctgttg gtcgccagct tgatggacgg cgtcggcttg cagccgtagg
     1201 tcgagccgcg gcccgtggtg aagcagatga ggttggcgcc gccggccacc tgcccggtgg
     1261 cggacacggg atcgtagccg ggcgtgtcca tgaagacgag tccgtgcgtg ccgaccggtt
     1321 cggcgtactc gaccacgtcc atcaatccgc tggtgccgcc cttggcgacc gcgccgagcg
     1381 acttctcgag gatggtcgtg atgccgcccg ccttgttgcc cggcgagggg ttgttgttca
     1441 tgtcgccgcc gttcttgccg gtgtagtcct cccaccagcg cagccgctgc atcaagcgct
     1501 cggcgatctc cgggcgcgcg gcgcgcgccg tgagcaggtg ttcggcgccg tagatttcgg
     1561 gtgtctcgga caggatcgcg gtgccgccat gacgcaccag caggtcgacg gccgcgccca
     1621 gcgccggatt ggcgctgatg ccggaatagc cgtccgaacc gccgcactgc aggccgacca
     1681 tcaggcggct cgcgggcacc ttctcccgcc gtgccgcgtt cgcgacggcc aggagctcgc
     1741 gcacgatggc gcagccctcg tcgatcgcct cgcgcgtgcc gccggcctcc tggatcgtca
     1801 tcgtgcggat gcggtcgccc gaggcgacga gcggctcgac gagcgcgccg acctggttca
     1861 cctcgcagcc caggccgatg accaggacgc cggcaaagtt cggatgcgtc gcatagccgc
     1921 gcagcgtgcg ccgcagcagc gcgatgcctt cgccgcggcc gctcatgccg cagccgccgc
     1981 catgggtgat cgcgacgacg ccgtcgacgc ccggccatgg cgccatggcg tcgccggcga
     2041 atgccttctc gatcgccttg cagacggtcg ccgagcagtt gacgctcgag atgacgccca
     2101 cgaagttgcg cgtgccgacg ctgccgtcgg accgcacgaa gccttcgaag ctcgccggct
     2161 cgaacaccgg cacggtcggc acgtaggcgc ggccgaccgc atgcgcggga tgcgactcgc
     2221 ccatgtcgag gttgtgcacg tggacgtgct ggccagcggc gatcgccacc gtcgcgacgc
     2281 cgatgacctg gccgtacttg cgcacggggg ccccctggcc gacggcgcgc agggcgatct
     2341 tgtggcccgc ggggacgtcg tcggcggcgc gcaggacgcc ttcgggaccg gcgagcaggc
     2401 tgccggccgc gacggcgtcg cgcgcgacgg ccacgtcgtc cgcgggatgc aggcgcagca
     2461 ggaccgaaga cgcagactca gtcgacatgg aacacctctt ccatcgccgc ccagtgctcg
     2521 ccgggcgcgc ggctgtccag cggacgctgg cacggatccg tgaatgtcca ccagcgctgc
     2581 gtctcggggt ccgcggcgat ggcggccatg tctgccgcga ggtcggtgcc gtggtattcc
     2641 cagcaagcga acagcaggtt ctccggctcg cgcaggtaga tcgagtagtt gcggatgccc
     2701 gacgccgcga tgcgctccag cacgccgggc cagaccgcgg cgtgcagccg cttgtattcg
     2761 gcgatgcgct cgggcgccac gccgatcacc atgcccattc gcttcatgtc gatgactcct
     2821 tcgtttcgag gcggctcgtc gaggcgaggg cgccgcgcag ccagggttgg gccagcgcgg
     2881 gcgcgcatcg ctgcgcggcc gcgacgacgg gcgccaggcg gcgctccttc ttctgtgcgt
     2941 ggttggcggc gatgtccgcg agccggtgcg cgagccaggg gttgagcagg cgttcgcgca
     3001 gcccggccag gtaggcgcgc gccgcgcggc cttcgcccag ggcctcgaac acgggaagca
     3061 cctcttcgcc ccaaagccgt tcgagcgcga ggcgcgtcga ttcgtcgcgc atcgcgtcca
     3121 gcaccgtgcg gccgtgcggc tgtccgtcgc gcagccagag ctccgcgagc gccgtgtggc
     3181 cgaggttgag cagccacagc ttgaggcgct cgaacgggcc caggtcctcc gtcaggacga
     3241 tgtcggcatg ccggcacggc ggcaccaggc ccggctggcg ttcgatggcc cagagcgcgt
     3301 agggctcggc gatggcgccg accggctcga tcgcctcggg gacgatgcga tcgaccagcg
     3361 agttcgccca gatcggacgc tcgcgcagcc acgcctggaa ggccgcatcc agctgccact
     3421 gcatggcaag ccccacgacg atgtcgcgca ggcgttcgcc attgcgcgcg acgagttcgc
     3481 atggcagcag cgtcagcgcg gcatccggcc gggcctgcca gcggtcgtga agggcgacca
     3541 gcagcttggc ggggaacgcg cgcggcgcgg cggcctgcgg cgccagcgcc tgcgggccgt
     3601 ccgtgtcgtc cagttgccag ccggcgtcgc cggtgttgga gacgacgacc tgcagggtgt
     3661 cggaggcgaa ctcgcgtcgc accgcaggcc agtcggtggc ggtgcgcagg gccgcgagga
     3721 cggcgtggca atggcgttgc tcgtcgacgg gccgcccgtt cgcgagccca cggatgcgca
     3781 ccgggtggcc tcggccgctg gccagtgccg ccacgcgtgc cgtgctgcgc ggatcgtcgg
     3841 tggtctggac gaccgtgatg ccgcccaggg catcgccgcg agccatcgcc tcggaaacga
     3901 gaagatcgac gtgcgcctgc aggaagcggc tggtgccgaa ctgcaggatc ggttggcccg
     3961 tcgacacggt cagcactcca cgatcgcctt gatgacgtgc tggcccggtt ccagcagggc
     4021 gggaaagtcg cgcggcaggt cggcgagctg catgcggtgg gtcgcgagcg ccgcgacggg
     4081 cacgtcgccg gcgcgcatgg cggcaagcac cgtctcgaag tccgccgtgg tcgcgttgcg
     4141 gctgctgagc agggtcgcct cgcgcttgtg gaactcgggg tcggcgaagg agatgtcgcc
     4201 ttgcacgatg gagaccagga catagcggcc gccgtgcgcg atgaaggcga acccgcgttc
     4261 catcgcctgc gggttgccgg tggcgtcgaa gacgacatcg aaatgctccc cgtcgctcag
     4321 gcgcgcaagt tcccgggcgt cgtcgggccc gacctcgacc gcggcctcga tgcccagatg
     4381 cgcgcaagcg acgtccagcc ggtcgcggcg ggtatcgagc gccacgacgc gggccccgcg
     4441 gcgggccgcg aacagcatca cggccaggcc gatcgggccg gcgccggcga cgagcacgcg
     4501 ctggccggag gcgacgtccc cccgcgccac ggcgtgcgcg ccgatggcca ggaattcgag
     4561 catcgccgcc tcgtccagcg tgatgccctc ggccttgtgg acgaacggcg cgggaaccgc
     4621 gaggtactcg gtcatcgcac cgtcgcgatg cacgcccagc acctggatcg acgtgcagca
     4681 gttggtcttg ccctggcggc aggcgacgca tcggccgcag gacagatagg gcatcacgta
     4741 gacggcgtcg ccgggggcca gcccgcttgc ggggtcggcc tcctcgacga tgcccgagag
     4801 ctcgtggccc atcacgcggg gatacgccag gtagggctgc ttgcccgtga agatgtgcag
     4861 gtcggtgccg cagacgccga cgcgccggac gcgcagcagc acctcgccgg gctggcgcgg
     4921 cgggcgcgga cgttgctcgg cgacgagctt gccgggggtt tcgcagagga cggtcagcat
     4981 gggcgtgagc cttggaggcg gtagagggtg acggcgttgt cgcccaggat ggcggcggct
     5041 tcctgggcgc tggcaggagg cgtcacggcc gccagcaagg cggtcgtcat cgcgagccag
     5101 ccggcgtagt ccccggccag ctgcagcacc ggccagtcgc tgccccacag caggcgcgac
     5161 gggccgaaca aggaccacag cgcctgcacg tacggcagca ggtccccgat gcgcgcgcga
     5221 tcgccggcct cggtgagcag gcccgagacc ttgcagtgca cgtgaggcag cgcggccagg
     5281 cgggccaggt cgtcctgcca cggcagcatc tgcccgcgcg cgatcggcgg cttggcggca
     5341 tggtcgacga cgacgagcaa ctgcggatga cgtgccgcga aggtgagcag ggcgggcagg
     5401 tgccgcggca gcaccagggc gtcgaacacc aggccgtggc gcgccatcgc ctgcgcggcc
     5461 ggcgcagtgg cgggatcggc gatccagtcg tcttcctcca ggtcctgcag catcggccgc
     5521 aagcctttca ggagcgggtc gcgggcgagc tccgcgatcg tgtcgacggc gtccggtgcc
     5581 ttcatgtcga cccagccgac gacgccgtgc acgaagcggg tcgtccgcgc cagcgcgagc
     5641 aagcggcgcg tctcggccac ggtggggagc gactgcacga ggacggtccc gtcgatcccg
     5701 ccggcctgca gcagcggggc caggtccccg ggctcgaagt cgcggtggat ggccgcgagc
     5761 gtcggcgggg gccaggcgcc gccacgctcg gcgagccgcc agaagtgctg gtgcgcgtcg
     5821 attctcatgg cctggctccg ccatcctgcg ggcatggcgc ggcgccctcc agaagcccct
     5881 ggtcgcgcag cgcatcccac aatccggcgg ggatcgcacg ctcgaaccag gcgacgttct
     5941 ggcgcacctg tgccggcgag cgcatgcccg agaggaccga gaccacggcg ggatgcccca
     6001 gcgggaactg cagtgccgcg gcttccagcg gcacgtcgaa atcagcgcat gtcgagcgca
     6061 gcgcggcggc gcgtcgcagg agctcgggcg gcgcgtcggt gtagtcgaac ttggcctgcc
     6121 ccgcgagcag ccccgagttg aacgggccgc cgacgacgat cgcgtgatgg cgtcgcgcgc
     6181 actcgtcgag gaagggcagg ctgccctgct cgagcagcgt gtagcggcca gcgagcaggc
     6241 tgcagtccag ctcgcactcc tgcatggcct cccgcatcac ctgccattcg ttgaccccca
     6301 gcccgatcgc tgccacctgg ccatcgcgac gcagttcctc cagtgcgcgg aatccgccac
     6361 ctcgcgtcag ggcgtcccaa tgggcctcgt gcgcgtcgcc atgggtgacg cgtccgatgt
     6421 cgtggaccag cagcaggtcc acgcggtcca ggccgagccg ctgcaggctg tcctcgacgg
     6481 agcgcaggat ggcgtcgcgt ccatagtcga acgccggccg gaacggcagc ggccgcgccc
     6541 atccgtcgct gcccgggcgc accgacgcgt cgggccgcat gagccggccc accttggtgc
     6601 tgatcgtgaa ctcgtgccgc ggctgctccc gcaggaatgc cccgacacga tgttcaccca
     6661 gcgtgtagcc gtagtagggc gccgtgtcga tgtagcgcag gccggcatcg cgggcggcgg
     6721 cgagcgtgtc cgcggcctcc tgttccgtga cgggcgcgta catgcccgcc agcgcggcgg
     6781 cgcccaggcc gagcgcggtc aggcgcagtc ggcccccggc gagcgtgcgg cgatcggcgg
     6841 ccttcatggc gtcaatacag gacgttcttg gccgcgggcg tgtcgatgtt ggtcttggtg
     6901 accatcacga cgccggtgtc gcggaacttg ggcaccggct tcttgttcag gacgtccacc
     6961 gccgtcttca cgccgaggtt gcccatctgg taggcactct gcaccgcgat cgacgagatc
     7021 gtgccgtcct tgacgaagcc ctggaggtcg gcattgccgt cgaagccgat ggccaccacc
     7081 ttgccggcct tgccggcctg caccagcgcg cgtcccatgc cgatggcggt gggctcgttg
     7141 gcgccgaaga tgcctttgag gtcggggttg gccgcgagca cgtcggtggt ctggttgagc
     7201 gccgtgccca tctgggagtt ggagtagaac ggcccgacga tctggagctt ggaatgcgcc
     7261 tcgatgtact tgcggaatcc gcccacccgg ccgatctccg agcccgagcc cgcggtgtac
     7321 gacatgatcg cgatcttgcc cgtcgtgccg atacgctcga tcagcgcctg cgcgcacagt
     7381 tcgccggcct tgttgttgtc cgtcgcgagg aaggactggt agtagtcctt gccgctgtcg
     7441 gcggcggccg agtcgatcag gatgaccggg atgtgcgcct cccaggcctt cttgatcgcg
     7501 gggatcaggg cttcggggtc cgacggcgcc agcacgatcg cggcgacctt gcggttgacc
     7561 gcgttctcca ccagcgcgac ctgcccggcg aggtcggtgt cggaggccgc gccctggaag
     7621 ctggcggtgt agcccttgag ctccgaggcg gcctgggtcg cgcccttgcg gacgttctgc
     7681 cagaagtccg agttggcggt cttgacgatg accgcgatct ccttgtcggc cgcggtggcg
     7741 gcggcccccg cgagcagcag cgcggaggcg agaacggcgt ggaagagtct tttcatggca
     7801 tgtccttctt cgaagtggga gaaccttgtc tcgagggtcc gcgggaggag acgcacccgc
     7861 cgcgtttcat cgtcgattgc gcaactggtc gatccagacg gtgaccagga tgaccaggcc
     7921 gatgatgatc tgctggacga aggccgagac ccccgtcatg ttcaagccgt tgcgcaggat
     7981 gccgatcacg aacgcgccga tcagcgtgcc cgagaccgtg ccgatgcctc cggtcagcga
     8041 ggtgccgccg atcaccgccg cggcgatggc gtcgagctcg tacatcacgc cctcgctggg
     8101 ctgggcggtc accaggcgcg acatcagcac gcacccggtc agtcccgcga gcgcgccgga
     8161 cagcacatag gccagcagcg tgacgcggcg cacgtcgatg cccgacagcc gcgccgcctc
     8221 cgcgttggat ccgaccgcgt agaggtgacg cccgagccgg ctgcgcccga gcatcagccc
     8281 gatggcgagc gccagcgcga tcatcagcag cacggggtag gggatgcccg ggaagacgac
     8341 atcgggaaag ccgtcggcgc cgatgcgcac gatccgccag agcgagccgt tgccgagcgc
     8401 ggcgaactgc tcgccgaggc cgccgacggc cttcgcgtcc gtgatctgca gcgccacgcc
     8461 gcgcgccacc agcatcatgg ccagcgtggc gatgaagggc ggcaagcgca tcaccgtgac
     8521 gcacaggccg ttgaccaggc cgcaaccgac ccccgtcaga atccccagca gcatggccag
     8581 cggcacgttg acgccgccgc gaacggccag ggccgcgacg acccccgaca gggccagaat
     8641 cgagcccacc gacaggtcga tgccgccggt gatgatgacg caggtcgcgc cgatgcccag
     8701 ataggcgatg gaggtgacct gcagggcgac ggtcatgccg ttgcccacgc tgaagaacgc
     8761 cgggctggcg agggagaaga ccgtcaccag gacgaacagg ccgccgagcg cggcgaactt
     8821 ctggacgaga tccttctggc gttcgttcat gggacgagcg attcctgttg cggcaaggcg
     8881 gcccggggca ggcacgtcga ggcgaagtgc atgatctctt cctgcgaggt ggcgcgggtc
     8941 ttgagcaccg tcgtgatgcg gccaccgtgg aagacggcga tccggtcggt catgccgagg
     9001 atctccggca attcggagct gatcatcacg acgccgatgc ccgcgcaggc gagctggtcc
     9061 agcagttcgt agatcgccac cttggcgccc acgtcgatcc cacgcgtggg ctcgtcgaag
     9121 aacagcacgc gcgaatcgcg gaacagccac ttggcgatga cgaccttctg ctggttgccg
     9181 ccggacagca gcctggcgac ctggtccggc gacggcgtgc ggatgcccag ctgttcgatg
     9241 tagcggcgcg cgatcgcgat ttcctcgtgc gcgcggatga ggccgcgcca gtccgtcacc
     9301 gccgccgtgt tggcgagcgt gatgttcgtc gcgacggaca tcttcaccgc caggccgtgg
     9361 gccttgcggt cctccgacag gtaggcgatg ccttccctga tggcgtcgat gggcgaccgc
     9421 acggtgagcg agcggccgcc cagctcgatg tgcccgccgt cgatcggatc ggcgccgaag
     9481 acggcgcgcg cgacctccgt gcggccggcg cccatgaggc ccgcgaagcc caggatctcc
     9541 ccgcgacgca gctcgaagtc gaccggcccg aacacgccct ggcgacgcag cccgcgcacc
     9601 cggagcaccg tctcggccgt gggctgccgc cgcgcgggcg ggaactgctt gtcgagcgtg
     9661 cgcccgacca tgagcgccac gatctcggcg accgatgtct cggcgaacgt cttcgtcgcg
     9721 atgtagcggc cgtcgcgcag cacggtgacg cgatcgacca ggccctccaa ctcgtcgagc
     9781 cggtgcgaga tgtagacgat gcccgagccg gcgcgcttga gctcgtggat gacacggaac
     9841 aactgggtcg tctcggcatc ggtgagagac gaggtgggct cgtccatgat gagcaggcgc
     9901 gcccgcatcg acagcgcctt ggcgatctcc accatctgct gctgtgccac cgacaggccg
     9961 gcgaccagtt cgtccggcga gatcgatacg cccagctggt cgaggcaggc ctgcgcatcg
    10021 gcgcgcagct tgcgccggtc caccagccac ccgcgcagcg gctcccggcc caggtagatg
    10081 ttctcggcga cgctcaggtg gggcaccagg ttcagctcct ggtggatcat cgcgatgccc
    10141 tgctcctgcg cctcgcgggg cgaagcgaag cgccgcggtt cgccgtccag cttcagcgtt
    10201 ccttcgtcgg gcgcgtagac cccgctcatg accttcatca gcgtggactt gccggccccg
    10261 ttctcgccac agacggcgtg cacctcgccg ccacgcacgt cgaagctcac gccttccagg
    10321 gcgacgacat tgaagaagcg cttggtcacg ccgctcaggc agagcagggc aggcgtggcg
    10381 tcgttcgtcg atgtcatgga agagacggcg attggtcagt ccaaactggt catgccaatt
    10441 tgccacgatt taaatttggc ctgaccagtg gaaacccgga acaacgccgc cacggccatg
    10501 agcgagactg tgcgcgacgg ctgctgcggg ccggtcgatc gcatcgcggc ggcgacgtgg
    10561 tcaggccacc gccgggcagt cccgcgattc tttccagttc ggagcgtcca tgactgccac
    10621 gccaaagccg gtcgacaccc gccgcctcta tcaacaggtg gccgaccgca tccgcaccgt
    10681 catccgtgaa gggcaatacg aggtgggctc gcgcctgccg cccgagcgcg agctggcact
    10741 gcaactgggg gtgtcgcgcc cgtcgctgcg tgaagcgctg atcgcgctcg agatcgacgg
    10801 ccggatcgag atccgcatgg gctcgggcgt ctacgtctgt gcaccgccgg tggcggcgga
    10861 gcgccacacg ccggccctgg gcgaaagccc gaccgagctc atgcaggccc gcgccacgct
    10921 cgaaggggcc gtcgtggcgc tggccgcggc gcgcgtgacg cgcgcgggcc tggaccgcgt
    10981 cgccgatagc ctggccgcga tgaagcgcga gcacgcgcgc ggccgcatgc ctgtcgaagc
    11041 cgaccgccgt tttcacctgg gcatcgccga actggccggc aacacggtgc tcgtgcgcat
    11101 ggtgggtgag ctgttcgacg gccgtcacag cccgatctcg tcgcgcatga gcggccatgc
    11161 cgagacggtg cagacctgga ccgtggcact cgcggagcac gagcgcatct ttcgtgccct
    11221 cgaggcgcgc gacccgcagg ccgcgacggc ggcgatgttc acgcacctgc gcgcttccga
    11281 ggaacggtgg gtgagcgagc ccgccgcctc catcgagtcg gcgccggcct tcccgaccat
    11341 caaggagcga ccatgagact cgcaggcaag acggccttca tcaccgccgc cggcaacggc
    11401 atcggtcgcg ccgcggcgat cgccttcgcc gccgaagggg cgcacgtcat cgccaccgac
    11461 gtcgacggcg cggcgctggc gtcgctggat cacccggcac tgcgcacgca cgtcctcgat
    11521 gtgcgcgacg gcgacgccgt gcggcgcgtc gccggtgctt ccgggacggt cgacgtgctg
    11581 ttcaactgcg ccgggatcgt ccaccacggc acgctgctcg aatgcgacga cgaggcctgg
    11641 gcgcggagca tggacatcaa tgtcggcagc atgcaccgcg tcacgcgggc gttgctgccc
    11701 ggcatgctgg ccgccggacg gggcagcatc atcaacatgg cgtcgatggc ctcgagcatc
    11761 aagggcttcc cgaaccgctg cgtctacggc gccagcaagg cggccgtgat cggcctgacc
    11821 aaggccatcg cggccgatca tgtccgccag ggcatccgct gcaacgcggt gtgtcccggc
    11881 acggtggaca cgccgtcgct gcgcggccgc atcgccgcgg ccgccgatcc ggcgcaggcc
    11941 gagcgcgact tcatcgcgcg gcaaccgatg gggcgcctgg ccgtcgtgga cgacatcacg
    12001 ccgctcctgg tctacctggc gagcgacgaa tcccgcttcg tgaccggaca ggcgctgtcc
    12061 gtggacggtg gcgtcaccat ttgaggagcg attccgatga aacttcttcg ccacgggccg
    12121 gccggccttg aaaagccggg catgctcgac tcgcaagggc aggtgcgcga cctcgccggc
    12181 atcgtcaggg acatcgccgg agcgacgctg accgatgcgg gactggcgtc gctgcggatg
    12241 ctggatccgg ggaccttgcc gctggtggcg ccgggcacgc gactcgggcc ctgcgtgggg
    12301 gcggtcggca agttcgtgtg catcgggctg aactacgcgg accacgcggc cgaatcgggc
    12361 atgcccattc ccgcggagcc ggtcgtcttc ggcaagtgga cgagcgccat cgcggggcct
    12421 gacgatgcca tcgagattcc ccgcggctcg acgaagaccg actgggaggt cgagctcggc
    12481 gtggtcatcg gcgaaggcgg ccgccacatc gcgcaagcgg atgcgttgcg ccacgtcgcc
    12541 ggctattgcg tcgtcaatga catctccgaa cgcgagtacc agctggaacg gggcggccaa
    12601 tgggacaagg gcaagggctg cgacaccttc ggcccgatcg gcccctggct cgtgacgcgc
    12661 gacgaggtgc ccgatcccca ggggcttggc ttgtggctcg acctcaatgg ccggcgcatg
    12721 caggacggca acacgcgcac gatggtgttc ggcgtcgcgc acatcgtgag ctacctgagc
    12781 cgcttcatga gcctgcagcc gggtgacgtc atctccaccg gcacgccgcc cggcgtcggc
    12841 ctgggccaga agccgccgct gtacctgcgc gctggcgacg tgatgcggct gggcatcgaa
    12901 ggcctcggcc agcagacgca gcgggtcatc gacgccgcat gaaggagggg cgcgaggtcg
    12961 ccgcctgcgg gccgacatgc caggccgtgg tcgcgggcaa ggtgattcat ccaccgcggc
    13021 agtccggaaa ggccgggcgg atgcgaccgg tcagggcttg accggctgca gcgtcagcag
    13081 cgcgatgtcg ttgctgtgca tcggcaccgt gaagtccaga agcccgttct tcccgatggt
    13141 gaccgtgcgc acggattcgg gccggtccag cgtcagcgat tgcagccgcg cgagctgggc
    13201 gtcatcgagt tgctgcggcg cgcccatctc gaggtaggcg gtgtaggcgt cgttggcccg
    13261 gtagccggtc cggcgcagct ccagctggta g
//
LOCUS       DATCRO010000689         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_919302, whole genome shotgun sequence.
ACCESSION   DATCRO010000689 DATCRO010000000
VERSION     DATCRO010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919302"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VIN75_18675"
     CDS             <1..441
                     /locus_tag="VIN75_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976246.1"
                     /translation="GAFGLAGAAGALAAPLAGRLADTRGPELVTRLGTGLAAVSFLVM
                     AAGALLPADLQLWVIGVGAVGFDLGAQATLVSHQAIVYGLDAHARSRLNALLFTGMFI
                     GMASGAALGSLALAQWEWTGVMLVAAASALAALAVRLWPARAGA"
     gene            complement(449..901)
                     /locus_tag="VIN75_18680"
     CDS             complement(449..901)
                     /locus_tag="VIN75_18680"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY8976247.1"
                     /translation="MTPSIIAQPALRLVGLQILTKPMSPEIPALWLRFAPRMPEVASP
                     MEPGVTFGVMQGAGDDLIYLAGLEVAPGAAPPAGMSQVAVPAGDYAVFEFPFGEIGKA
                     YPFIFGTWLPESGHVQDARPLLERYGTDFCPDQPGSPMQVRVPVRPRA"
     gene            complement(1293..1721)
                     /locus_tag="VIN75_18685"
     CDS             complement(1293..1721)
                     /locus_tag="VIN75_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8976248.1"
                     /translation="MTTTIATASISKDAAQALIAAANAASRDLGIAVTIAITDAGGHL
                     TALARDDGAPFLSVNVAIDKAWTATSFGLPTHTWADVIANPQVAQLAHQPRLVAVGGG
                     LPIVSAGRVVGGIGISGGNAAQDRQAAEVALKAVGFETAA"
     gene            complement(1723..>2302)
                     /locus_tag="VIN75_18690"
     CDS             complement(1723..>2302)
                     /locus_tag="VIN75_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8976249.1"
                     /translation="GGGKGQTLLVVGGAGGVGSILIQLARQLTQLKVIATASRPETRA
                     WCLELGAHAVVDHAQPLAQALEDGGHGQVDFVAALTQTGQHYEQIVQSLKPQGALAVI
                     DDMPVLDAMKLKTKAISLHWELMFTRSMFGTPDMDEQGKLLAEVARLVDAKRLRTTAG
                     AHYGIINAANLLRAHAFIESGKAQGKVVLEGF"
BASE COUNT          292 a          819 c          859 g          332 t
ORIGIN      
        1 ggcgcgttcg gcctggccgg agccgcaggc gctctcgccg caccgctggc cggccggctg
       61 gcggacacgc gcggcccgga gctcgtgacg cgcctgggca ccggcctcgc cgccgtctcg
      121 ttcctcgtga tggccgcggg cgcgctgctg ccggccgacc tgcagctgtg ggtcatcggc
      181 gtgggcgcgg tcggcttcga cctcggcgcg caggcgaccc tggtgtcgca ccaggcgatc
      241 gtctacggcc tggacgcgca cgcgcgcagc cgcctgaacg cgctgctgtt cacgggcatg
      301 ttcatcggca tggccagcgg cgccgcgctc ggcagcctgg cgctggcgca gtgggagtgg
      361 acgggcgtga tgctggtggc ggccgccagc gcgctcgcgg cgctcgccgt gcggctgtgg
      421 cctgcgcgcg cgggcgcctg agcgcgcgtc acgcgcgcgg ccgcacgggc acgcgcacct
      481 gcatgggcga gcccggctgg tcgggacaga agtccgtgcc gtagcgctcc agcagcggcc
      541 gcgcgtcctg cacatggccc gactcgggca gccaggtgcc gaagatgaac ggatacgcct
      601 tgccgatctc gccgaacgga aactcgaaga cggcgtagtc gccggccggc acggccacct
      661 gcgacatccc ggccggtggc gccgcgcccg gcgcgacctc caggccggcc aggtagatca
      721 ggtcgtcgcc cgcgccctgc atgacgccga acgtgacgcc cggttccatg ggcgatgcga
      781 cctccggcat ccgcggggcg aatcgcagcc acagcgcggg gatttcgggc gacatgggct
      841 tggtgaggat ctgcagcccg acgaggcgca gtgcgggttg ggcgatgatg gacggtgtca
      901 tgtcggctcc cgtgatgaag gttcacacct tccacgacgg atcggcgggt gcggtttcga
      961 catcggctgg cgacctgcaa gccgagaggg ccggcctctc cgcaagttgc cgccgggatg
     1021 tcgccgtcgg ccatgacgac gtcggcaaca tgcggcgaag gcctgacacc gaacgtcagc
     1081 gacggatcgg tgtcaggcct tcgcctccac atcgcgacag ccgcttcggc cccgtggtcg
     1141 cacagcggcg cgatgtcgag gagaggccag atccctccgt tggacggggc gccgctcgac
     1201 gcgccgggcg gggcgatcac gccgatgcgc acgcgggtca ccgcgacgac gtcgccgagc
     1261 tcctgttcca cgggccgtcg acgcgcagcg cgtcaggccg ccgtctcgaa gccgacggcc
     1321 ttgagcgcca cctccgcggc ctgccggtcc tgcgccgcgt tgccgcccga gatgccgatg
     1381 ccaccgacga cgcgacccgc gctgacgatg ggcagcccgc cgcccaccgc caccagccgc
     1441 ggctggtgcg cgagctgcgc gacctgcggg ttggcgatga cgtcggccca ggtgtgcgtg
     1501 ggcaggccaa aggacgtggc cgtccacgcc ttgtcgatcg cgacgttgac gctgaggaag
     1561 ggcgcgccgt cgtcgcgcgc gagcgccgtg aggtgcccgc ccgcgtcggt gatcgcgatg
     1621 gtgacggcga tgccgaggtc gcgcgaggcg gcgttcgcgg ccgcgatgag tgcttgcgcg
     1681 gcgtccttcg agatggaggc ggtggcaatg gtggtggtca tctcagaagc cctccagcac
     1741 gaccttgccc tgcgccttgc cgctctcgat gaacgcgtgc gcgcgcagca ggttggccgc
     1801 gttgatgatg ccgtagtgcg cgccggcggt ggtgcgcagg cgcttggcgt cgaccaggcg
     1861 cgcgacctcc gcgagcagct tgccctgctc gtccatgtcg ggcgtgccga acatcgagcg
     1921 cgtgaacatc agctcccagt gcagcgagat cgccttggtc ttgagcttca tcgcgtcgag
     1981 cacgggcatg tcgtcgatga cggccagcgc gccctgcggc ttcaggctct ggacgatctg
     2041 ctcgtagtgc tggccggtct gcgtcagcgc ggcgacgaag tcgacctggc cgtggccgcc
     2101 gtcctcgagc gcctgcgcga gcggctgcgc gtggtcgacg acggcgtggg cgccgagctc
     2161 caggcaccac gccctggtct cgggccgcga cgccgtggcg atcaccttca gctgcgtcag
     2221 ctggcgcgcc agctggatca ggatcgagcc gacgccgccg gcgccgccga cgaccagcag
     2281 cgtctgcccc ttgccgccgc cc
//
LOCUS       DATCRO010000690         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_919307, whole genome shotgun sequence.
ACCESSION   DATCRO010000690 DATCRO010000000
VERSION     DATCRO010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919307"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIN75_18695"
     CDS             <1..588
                     /locus_tag="VIN75_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8976250.1"
                     /translation="VDFVTLSGTAGTGKTLATLACGLAQVMDDRRYSEIIVTRVTVPV
                     GEDIGFLPGTEEEKMGPWMGALDDNLEVLSKGDGGAGEWGRAATQDLVRSKIKVKSLN
                     FMRGRTFLNKYVIIDEAQNLTPKQMKTLITRAGPGTKIVCLGNLAQIDTPYLTEGSSG
                     LTYAVDRFKGWPHGGHITLARGERSRLADFASDAL"
     gene            complement(656..>2542)
                     /locus_tag="VIN75_18700"
     CDS             complement(656..>2542)
                     /locus_tag="VIN75_18700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8976251.1"
                     /translation="DHGPQVASTCTAGLEGAMLRLKELEKRKPGADWLKGWEALYDWE
                     EDQSGALIFLQNVHPNPDVRTESEKCEQRWTAFQTALGMDEQVYQGARQTPALLKDPV
                     DKRAAQRALEGFEDAGVGLSRDKQARAKAIAEKLAALGQQFSQNINGAHVRVGFTEAE
                     LKGVPESLWKDLPRDSEGKIALPIDGPAYTIVMQTADDAGARERLWRAKTNEGGPDNL
                     KVLAQIEQLRQEQARLFGLASYDDFVLRRRMVESTAQATKFLDDVRTAAADEDRHNVI
                     ELRQAKARHLGKTLAETTLQRWDTMYYAERIERERIGLDDEAFRPYFPPQESLRFVMR
                     VAEKMFGIRYDKAANTNVWAPDVQAWAVTDVASNKKIATLFVDPYPREGKYGHAATWN
                     FRNAATSVPRQAQSALVVNFDRRGLTLRELETLMHEFGHTLRNNLSATRYASDAGENV
                     AQDFAEAPSQMLEDWVYDKKVLKVMQEVCPACKPVPDELVDKARAARDFGKGMQVARQ
                     QLYAAYDLALHGPSAPDPMETWEKMEAATSLGYVQGTTFPTGFAHIAGGYGAGYYGYL
                     WSQAVAADMRTAFAADKLDPKTGARFRATVLAEGAQKPPQQVVKEFLGRDGNAQAFFD
                     WLKK"
BASE COUNT          347 a          861 c          897 g          437 t
ORIGIN      
        1 gtcgacttcg tcacgctgag cggcaccgcc ggcaccggca agacgctggc gacgctggcc
       61 tgcggcctgg cgcaggtgat ggacgaccgc cgctacagcg agatcatcgt cacgcgcgtg
      121 accgtgcccg tgggcgagga catcggcttc ctgcccggca ccgaggaaga gaagatgggc
      181 ccctggatgg gtgcgctcga cgacaacctc gaggtgctgt ccaagggcga cggcggcgcc
      241 ggcgaatggg gccgcgcggc cacgcaggac ctcgtgcgct cgaagatcaa ggtcaagagc
      301 ctgaacttca tgcgcgggcg caccttcctc aacaagtacg tgatcatcga cgaggcgcag
      361 aacctcacgc ccaagcagat gaagacgctg atcacgcgcg cgggccccgg caccaagatc
      421 gtgtgcctgg gcaacctcgc gcagatcgac acgccctacc tcacggaagg cagctcgggc
      481 ctgacgtatg cggtcgaccg cttcaagggc tggccgcacg gcgggcacat cacgctggcg
      541 cgcggggaac gttcgcgcct ggcggacttc gcgtcggacg ccctgtagag gcggcgcacg
      601 atctcgcaac aagggcggct tcggcggccc ttgtccattc gggggcgcac gggccctact
      661 tcttgagcca gtcgaagaat gcctgcgcgt tgccgtcgcg acccaggaat tccttgacga
      721 cctgctgcgg cggcttctgg gcgccctcgg ccaggacggt ggcgcggaag cgcgcgccgg
      781 tcttggggtc gagcttgtcg gcggcgaacg cggtgcgcat gtcggcggcc accgcctggc
      841 tccagaggta gccgtagtag ccggcgccgt agccgccggc gatgtgcgcg aagccggtgg
      901 ggaacgtcgt gccctgaacg tagcccagcg acgtggcggc ctccatcttc tcccacgtct
      961 ccatcggatc gggcgcggac gggccgtgca gcgcgaggtc gtaggcggcg tacagctgct
     1021 ggcgggcgac ctgcatgccc ttgccgaagt cgcgcgcggc gcgcgccttg tcgacgagct
     1081 cgtcaggcac cggcttgcag gcggggcaaa cctcctgcat gaccttgagc accttcttgt
     1141 cgtagaccca gtcctccagc atctgcgacg gcgcctcggc gaagtcctgg gcgacgttct
     1201 cgccggcgtc gctcgcgtag cgcgtggccg acaggttgtt gcgcagcgtg tggccgaact
     1261 cgtgcatcag cgtctcgagc tcgcgcagcg tgaggccgcg gcggtcgaag ttgacgacga
     1321 gcgccgactg cgcctggcgc ggcacgctgg tggcggcgtt gcggaagttc caggtggcgg
     1381 cgtggccgta cttgccctcg cgcgggtacg ggtcgacgaa cagcgtggcg atcttcttgt
     1441 tgctggccac gtcggtgacg gcccaggcct ggacgtcggg cgcccacacg ttggtgttgg
     1501 ctgccttgtc gtagcggatg ccgaacatct tctcggccac gcgcatcacg aagcgcaggc
     1561 tctcctgcgg cgggaagtac gggcggaacg cctcgtcatc caggccgatg cgctcgcgct
     1621 cgatgcgctc ggcgtagtac atggtgtccc agcgctgcag cgtggtctcg gccagcgtct
     1681 tgcccaggtg gcgcgccttg gcctggcgca actcgatcac gttgtgcctg tcctcgtcgg
     1741 cggcggccgt gcgcacgtcg tcgaggaatt tcgtggcctg cgccgtggat tcgaccatcc
     1801 ggcggcgcag cacgaagtcg tcgtagctgg cgaggccgaa cagccgggcc tgctcctggc
     1861 gcagctgttc gatctgggcc agcaccttga ggttgtccgg gccgccctcg ttggtcttgg
     1921 cgcgccacag gcgctcgcgt gcaccggcgt cgtcggcggt ctgcatcacg atggtgtagg
     1981 ccgggccgtc gatgggcaac gcgatcttgc cttcggagtc gcgcggcagg tccttccaca
     2041 gcgactcggg cacgcccttc agctcggctt ccgtgaaccc gacgcgcacg tgcgcgccgt
     2101 tgatgttctg gctgaactgc tggccgagcg cggccagctt ctcggcgatc gccttggcgc
     2161 gcgcctgctt gtccctggac aggcccacgc cggcgtcctc gaaaccctcg agcgcgcgct
     2221 gcgcggcgcg tttgtcgacg gggtccttga gcagcgcagg cgtctgcctg gcgccctggt
     2281 acacctgttc gtccatgccc agggccgtct ggaatgccgt ccagcgctgc tcgcacttct
     2341 cggattcggt gcggacatcc gggttcggat gcacgttctg caggaagatc agcgcgccgc
     2401 tctggtcttc ttcccagtcg tacagcgcct cccagccctt gagccagtcg gcgcccggct
     2461 tgcgcttctc cagttccttc aggcgcagca tcgcgccctc caggccggcg gtgcaggtgg
     2521 aggcgacctg cgggccgtgg tc
//
LOCUS       DATCRO010000691         5999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1087401, whole genome shotgun sequence.
ACCESSION   DATCRO010000691 DATCRO010000000
VERSION     DATCRO010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087401"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VIN75_18705"
     CDS             complement(<1..298)
                     /locus_tag="VIN75_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEY8976252.1"
                     /translation="MIDSLSGAAVAPIAWTDADRQQAFGRWFDELAPRHGLDRSTLHL
                     ASADASSRRYFRVTGAGGATFIVMDSPAAEDSVAAFVHIGGLILGAGLHAPRVLE"
     gene            556..2994
                     /gene="lptD"
                     /locus_tag="VIN75_18710"
     CDS             556..2994
                     /gene="lptD"
                     /locus_tag="VIN75_18710"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY8976253.1"
                     /translation="MRSFPSRRHRRPPSALAPLAVAAALCAFASGAHAQALPRVRTTP
                     PPEPPKEAPGADVAPPLVTLTADDVVSHIGGVTQASGKVEVKRLDTTLFADLLTYDRL
                     SDTAHGQGHVRIARGLDWFSADRVDLELTRNAGTLVGTDYELGARKAGGHADRIELRD
                     RNHSTAYNANYTSCTRDGPDEPDWIISGSQIDIDTTANEGRATHAVLHFLGVPILYAP
                     SLTFPVTAERKSGWLPPTGDLSNRSGFTLSVPWYWNIAPNIDATLSPGYATKRGVELT
                     GELRYLEPDDLGQVTAYGLPNDEVYGDPRGAVEWAHEGKRGDWLSYSARVQRVSDADY
                     WKDFPNQMPALTQRLLPIDLSGARRFLPFGDAGEIDAYARVQRFQTLQDRGEADDASR
                     IQVPYQRSPQLGLRGNVTLKDQVRLDFEAEANRFDLSGLRQENGYIDFVNEAIRAQAP
                     DPSKATLVAHDQRVGGNRAHVVGSISRNFATDWGRFEPRMTVHGVSYETDTQADGTRL
                     DATRVIPTFSADSSFSFERQAELFGRDLVQTLEPRFLYVLTPYHDQGRLPLYDTALKD
                     FNEVSIYSENQFTGQDRVNDSNQLTAGVTTRFNDSGNGRELLRLGIAQKLLFKDQQIT
                     SDGSVDSHKLSHLLLYGSSSAMEHWSFDGIVEEDPSSKEGWTQRAVVSARFHPGPFRS
                     MSVTYRYARDSSKQWELGGQWPVFRREPRPSGCGGTLYAVGRADYSVSDRRATYAIGG
                     FEYDAGCWIGRVMVERTSTGRNEGTTHLVLQLELNGLSTLGTGSLKILKDNVPGYQPL
                     RNDPGAPGGTTNSP"
     gene            2991..4436
                     /locus_tag="VIN75_18715"
     CDS             2991..4436
                     /locus_tag="VIN75_18715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8976254.1"
                     /translation="MRDRLKLNFLTLVAATAALLSASAGFAQKVPDAGRPATPATAAP
                     EADVNKPALVSPVAASGVRQFGSLPRIKPLAKSADYIVAVVNSESVTDNEVKFRVAKM
                     QQESIERGGAQPTAAELRKQALDALVEERVLVTYARDNGVKVDDGEVDRAIANIASSN
                     KMTPEQLRDRLRQQGVDFNTFKSGIRDQITIERIREREVVNHIRVTDAEIADYIAQMR
                     GGHTAQPQVNIAQILVTVPDKATPAVEAERKARAEAALARVRNGEPFEQVVRDMSEDS
                     NKAKGGEMGSRSLDKYPDLFANAIKDVKVGGTTGLVRSGAGWHILKVVSRGEESGLTI
                     TQTRVRHIVLRPSAQMGIDAVERRILDFRQQIASGAKTFEELARQYSEDGSAQAGGDL
                     GWSSPGSFVPEFEEAMDKLPVGGLSGPVRSRFGVHLIQVLDRRDTTVDPKALREQATN
                     ALKEKKFEPAYAEWVADLRAKAFIEYRDAPV"
     gene            4640..5449
                     /gene="rsmA"
                     /locus_tag="VIN75_18720"
     CDS             4640..5449
                     /gene="rsmA"
                     /locus_tag="VIN75_18720"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8976255.1"
                     /translation="MTAHVARKRFGQHFLTATDVIDRIIRAIDPKPGERLVEIGPGLG
                     AMTIPLRLRCDALTVVELDRDLAARLRQRGDLDVVESDVLRVDFAALADRLGGPIRVV
                     GNLPYNISSPILFHLMPAAPHVVDQVFMLQKEVVDRMAAAPGSKAFGRLSVMLQWRYE
                     IEHLFDVAPDAFDPPPRVDSAIVRMRPRANPAPVPEAVLGRIVGIAFSQRRKMLRATL
                     GKWLAEHGWTGEFDGTRRAEQVPVDEYVALALSLPPLPAEALGGPDVGDDD"
     gene            complement(5529..5720)
                     /locus_tag="VIN75_18725"
     CDS             complement(5529..5720)
                     /locus_tag="VIN75_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976256.1"
                     /translation="MLLFFSVARSQENGSWERAAEAAPIIEKPAPKAGSKANANATFG
                     TNMPLMSSAFDTAMWLNAA"
     gene            complement(5699..>5999)
                     /locus_tag="VIN75_18730"
     CDS             complement(5699..>5999)
                     /locus_tag="VIN75_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY8976257.1"
                     /translation="FLYANLTNAQSKQDVLEGIAEAFLFPAHFGKNLDALYDCMTDLV
                     HKSGSQPGFVVVLEQLPDNPRFDRESREQLLDVFRDAADFWGERKIPFRCFYSFQ"
BASE COUNT          956 a         2150 c         2042 g          851 t
ORIGIN      
        1 gctcgaggac gcgcggcgcg tgcaggcccg cgcccaggat gagcccgccg atgtgcacga
       61 aggcggcgac gctgtcctcg gcggcgggcg agtccatcac gatgaaggtg gcgccgccgg
      121 cccccgtgac gcggaagtag cgccgcgagc tggcgtcggc cgaggcgagg tgcagggtgc
      181 tgcggtccag gccgtggcgc ggggccagct cgtcgaacca gcggccgaag gcttgctggc
      241 gatcggcatc ggtccaggcg atgggcgcaa cggcggcgcc ggagagggaa tcgatcatcg
      301 ggatgggagt gggctgcgcg ggaagaaagc gtggcgcggg gcaggctcgt gactcccggg
      361 caacgcggca cgaggcgcgg acacaaggcg gcagcactgt cgtggataat cgacattcta
      421 ataatccccc tgcgtcgcac gcggctcggg ttcccgcagg gcggtgtcga cgcgtcgatt
      481 cgctgtccgc cgcgcggggc cgccctccgc tgtcgcaagg cgttccgccc accccgtttc
      541 cagcccgatc gcgtgttgcg ttcttttccg tcgcgtcgcc atcgccgtcc cccgtccgcc
      601 ctggcgccgc tggccgtcgc ggccgcgctg tgcgcgttcg cgtccggcgc gcacgcccag
      661 gcgctcccgc gcgtgcgcac gacgccgccg cccgagccgc ccaaggaggc gcccggtgcc
      721 gacgtcgcgc cgcccctggt cacgctcacc gccgacgacg tcgtcagcca catcggcggc
      781 gtgacgcagg ccagcggcaa ggtggaggtc aagcgcctgg acacgacgct gttcgccgac
      841 ctgctcacct acgaccgcct cagcgacacc gcgcacggcc agggccatgt gcgcatcgcc
      901 cgcggcctgg actggttcag cgccgaccgc gtcgacctcg agctgacgcg caacgccggc
      961 acgctcgtcg gcaccgacta cgagctcggc gcacgcaagg ccggcggcca cgccgaccgc
     1021 atcgagctgc gcgaccgcaa ccactcgacg gcctacaacg ccaactacac gagctgcacg
     1081 cgcgacggcc cggacgagcc cgactggatc atctcgggca gccagatcga catcgacacg
     1141 accgccaacg aggggcgcgc cacccacgcg gtgctgcact tcctgggcgt gccgatcctg
     1201 tacgcgccgt cgctcacgtt cccggtgacg gccgagcgca agtccggctg gctgccgccc
     1261 accggcgacc tgagcaaccg cagcggcttc acgctgtcgg tgccctggta ctggaacatc
     1321 gcgcccaaca tcgacgccac gctgtcgccc ggctacgcca ccaagcgagg cgtcgagctc
     1381 acgggcgagc tgcgctacct cgaacccgac gacctgggcc aggtgaccgc ctacggcttg
     1441 cccaacgacg aggtctacgg cgatccgcgt ggtgccgtgg agtgggccca cgagggcaag
     1501 cgcggcgact ggctgagcta cagcgcccgc gtgcagcgcg tctccgatgc cgactactgg
     1561 aaggattttc ccaaccagat gcccgccctg acgcagcgcc tgctgccgat cgacctgtcg
     1621 ggcgcgcggc gcttcctgcc gttcggcgac gccggggaga tcgacgccta cgcccgagtg
     1681 cagcgcttcc agacgctgca agaccgcggc gaagccgacg acgcctctcg tatccaggtg
     1741 ccttaccagc gcagccccca actgggcctg cgcggcaacg tcacgctcaa ggaccaggtg
     1801 cgcctcgact tcgaagccga ggccaaccgc ttcgacctgt ccggcctgcg ccaggagaac
     1861 ggctacatcg atttcgtcaa cgaggccatc cgcgcacagg cgcccgaccc gtcgaaggcc
     1921 acgctcgtcg cgcacgacca gcgcgtcggc ggcaaccgcg cccacgtggt gggttcgatt
     1981 tcgcgcaatt tcgcgacgga ctggggccgc ttcgaaccgc gcatgacggt gcatggcgtg
     2041 agctacgaga ccgacacgca ggcggatggc acgcgcctgg acgcgacccg cgtgatcccg
     2101 acgttcagcg cggacagctc gttcagcttc gagcggcagg ccgagctgtt cggccgcgat
     2161 ctcgtgcaga cgctggaacc gcggttcctg tacgtgctca cgccgtacca cgaccagggc
     2221 aggctgccgc tgtacgacac cgcgttgaag gatttcaacg aggtctcgat ctacagcgag
     2281 aaccagttca ccggccagga ccgcgtcaac gactcgaacc agctcactgc cggggtcacg
     2341 acccgtttca acgattcggg caacgggcgc gaactgctgc gcctgggcat cgcgcagaag
     2401 ctgctgttca aggaccagca gatcacgtcc gacggcagcg tcgactcgca caagctgtcg
     2461 cacctgctgc tgtacggctc gtcgtcggcc atggagcact ggagcttcga tggcatcgtg
     2521 gaagaagacc cgtcctccaa ggaaggctgg acgcagcgcg ccgtcgtgag cgctcgcttc
     2581 catccggggc ctttccggtc gatgagcgtg acttaccggt acgcgcgcga ttccagcaag
     2641 cagtgggaac tcggcggcca atggccggtc ttccgccgcg agccgcggcc cagcggctgc
     2701 gggggcaccc tgtacgcggt cggacgggcc gactacagcg tgtccgaccg tcgcgccacc
     2761 tacgccatcg gcggcttcga gtacgacgcc ggctgctgga tcggccgcgt catggtcgag
     2821 cgcacgtcca ccggccgcaa cgaaggcacg acccacctgg tgctgcagct ggaactcaac
     2881 ggcctgtcga cgctcggaac aggatccttg aagatcctga aggacaatgt gcccggctac
     2941 cagccgctgc gcaacgatcc gggcgcaccg ggcgggacca cgaattcacc atgagagatc
     3001 gcttgaagct caatttcctg accctggttg ccgcgacggc cgcgctgctg tccgcctccg
     3061 ccggcttcgc ccagaaggtc ccggacgccg gccgtcccgc cacgcccgcc accgccgccc
     3121 cggaggccga cgtcaacaag ccggcgctcg tctcgccggt ggccgcctcg ggcgtgcgcc
     3181 agttcggctc gctgccgcgg atcaagccgc tggccaagtc ggccgactac atcgtggccg
     3241 tggtcaacag cgagtcggtc acggacaacg aggtgaagtt ccgcgtcgcc aagatgcagc
     3301 aggaaagcat cgagcgcggc ggcgcccagc ccacggcggc cgagctgcgc aagcaggcgc
     3361 tcgacgcgct ggtcgaggag cgcgtgctcg tcacctacgc ccgcgacaac ggcgtcaagg
     3421 tggacgacgg cgaggtcgac cgcgccattg ccaacatcgc ctcgtccaac aagatgacgc
     3481 ccgagcagct gcgcgaccgg ctgcgccagc agggcgtcga cttcaacacg ttcaagtccg
     3541 gcatccgcga ccagatcacc atcgagcgca tccgcgagcg cgaggtggtc aaccacatcc
     3601 gcgtgaccga cgccgagatc gccgactaca tcgcccagat gcgcggcggc cacacggcgc
     3661 agccgcaggt caacatcgcg cagatcctcg tcaccgtgcc cgacaaggcc acgcccgccg
     3721 tcgaggccga acgcaaggcg cgcgccgagg cggcgctggc tcgcgtgcgc aacggcgagc
     3781 cgttcgagca ggtggtgcgc gacatgtccg aggacagcaa caaggccaag ggcggcgaga
     3841 tgggctcgcg ctcgctcgac aagtaccccg acctgttcgc caacgccatc aaggacgtca
     3901 aggtgggcgg cacgaccggg ctggtgcgca gtggcgccgg ctggcacatc ctgaaggtcg
     3961 tctcgcgcgg cgaggagtct ggcctgacca tcacgcagac gcgcgtgcgc cacatcgtgc
     4021 tgcgcccgtc cgcgcagatg ggcatcgacg cggtcgagcg ccgcatcctc gacttccgcc
     4081 agcagatcgc cagcggcgcg aagaccttcg aggagctcgc gcgccagtat tcggaagacg
     4141 gcagcgcgca ggccggcggc gacctcggct ggtcctcgcc gggctcgttc gtgcccgagt
     4201 tcgaggaggc catggacaag ctgccggtcg gcggcctgtc gggccccgtg cgttcgcgct
     4261 tcggcgtcca cctgatccag gtgctggacc gccgcgacac caccgtcgac ccgaaggcgt
     4321 tgcgcgagca ggccaccaac gcgctcaagg aaaagaagtt cgagccggcc tacgccgagt
     4381 gggtggccga cctgcgcgcc aaggccttca tcgaataccg cgacgcgccg gtataaatga
     4441 cggcccccac gctccgagcg gtcgggacgc atccggcttg caggccggat gccgttccgg
     4501 cggggccttg ctgcgcaagt cctttgccgc cttcactccc ccaaggggga agccggttcg
     4561 gctcggatgc gatcctcgct tcccttgtgc ttgttgggcc tgcggcccgg gcgggcgccc
     4621 atgtgcggcg tggacagtca tgacggcgca cgttgcccgc aagcgcttcg gccagcactt
     4681 cctgacggcc accgacgtca tcgaccgcat catccgcgcg atcgacccga agccgggcga
     4741 gcgcctggtc gagatcggcc ccggcctggg cgcgatgacg atcccgctgc gcctgcgctg
     4801 cgacgcgctg acggtggtgg aactcgaccg cgacctcgcg gcccggctgc gccagcgggg
     4861 cgacctcgac gtcgtcgagt ccgacgtcct gcgcgtggac ttcgcggcgc tggccgaccg
     4921 cctgggcggc ccgatccgcg tcgtcggcaa cctgccctac aacatctcgt cgcccatcct
     4981 gttccacctg atgccggccg cgccgcacgt ggtcgaccag gtgttcatgc tgcagaagga
     5041 ggtggtcgac cgcatggccg ccgcgccggg cagcaaggcc ttcggccggc tcagcgtgat
     5101 gctgcagtgg cgctacgaga tcgagcacct gttcgacgtc gcgcccgacg ccttcgatcc
     5161 gccgccgcgc gtcgattcgg ccatcgtgcg catgcgcccg cgcgcgaacc cggccccggt
     5221 gcccgaggcg gtgctgggca ggatcgtggg catcgcgttc tcgcagcgcc gcaagatgct
     5281 gcgcgcgacc ctcggcaagt ggctggcgga gcacggctgg acgggcgagt tcgacgggac
     5341 gcgccgcgcc gagcaggtgc cggtcgacga gtacgtcgcc ctcgccctgt cgctgccgcc
     5401 gctgccggcc gaggcgctgg gcggccccga cgtcggcgac gacgactgac gcctgcgcac
     5461 gattgcgccg ccggaaatga agaagggctc ccgcgggagc ccttcgtgcg agtggacgcg
     5521 cctggtgttc aggccgcgtt gagccacatc gcggtatcga acgcgctgct catcaacggc
     5581 atgttggtgc cgaaggtggc gttcgcgttg gctttgctgc cggccttcgg ggccggcttc
     5641 tcgatgatcg gggccgcttc tgccgcccgc tcccatgacc cgttttcttg ggagcgggct
     5701 actgaaaaga atagaagcac cggaagggaa tcttgcgttc cccccagaag tcggccgcat
     5761 cccggaagac gtcgagcagc tgctcgcgcg attcacggtc gaagcgcggg ttgtccggca
     5821 gctgctccag cacgacgacg aagccgggct gggagcccga cttgtgcacg aggtcggtca
     5881 tgcagtcgta cagggcgtcc aggttcttgc cgaagtgggc cgggaacagg aacgcctcgg
     5941 caatgccttc gagcacgtcc tgcttggact gcgcgttggt caggttcgcg tagagaaaa
//
LOCUS       DATCRO010000692         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_30228, whole genome shotgun sequence.
ACCESSION   DATCRO010000692 DATCRO010000000
VERSION     DATCRO010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30228"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VIN75_18735"
     CDS             <1..473
                     /locus_tag="VIN75_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976258.1"
                     /translation="GLNLGLPLGGATVGVHALTGRIGSPVDGQSLTAMRILGGYAVYD
                     EDNWEAMAEYFGFVDRNVVGGGLHTSHAAYAQVGRSFGAVTPYARYELASLDQGDDYF
                     ASMIGGRSYRRALVGARFDIDPRSTLKLEFDHTSLTDRIKDRYRESRVEYAIRF"
     gene            481..885
                     /locus_tag="VIN75_18740"
     CDS             481..885
                     /locus_tag="VIN75_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976259.1"
                     /translation="MAGKAHFLVLGVAALLAGAAPGAARAQAVITNTGVTIVAADVRM
                     IYIGEKQFTGAVRLIPVDNSAVQDRFLAAFLQVDNGKYYSIWAKKSFREGVNPPLLKA
                     TDGEVVDFVRRTPGAIGYIGDPSKAVGVTIVK"
     gene            882..1442
                     /locus_tag="VIN75_18745"
     CDS             882..1442
                     /locus_tag="VIN75_18745"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8976260.1"
                     /translation="MNANRLARRSVLGTLAALALPASAVAPQATANVWSLHGQWTDDQ
                     GHSVAMADWAGRPALLTLAYGSCRRICSTSLRLLERAQQEADARKVDLSVIVVSLDPR
                     VDTPASWREYRAEHRLQRANWHFLDGTPALTRRVADLLEMQYWIYDDHVLHDLRIAAV
                     DVSGRIVARIDWVDEPPRRLIDALAS"
     gene            complement(1446..2393)
                     /locus_tag="VIN75_18750"
     CDS             complement(1446..2393)
                     /locus_tag="VIN75_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEY8976261.1"
                     /translation="MNFQQLRSVREAVRRGFNLTEVAQALHTSQPGVSRQIRELEEEL
                     GVEIFVRVGKRLTGLTQPGAAVLPWVERLLQDAESLQRASEEFTQGTTGVLTIAATHS
                     QARYALPTAVRDFRAAHPGVQLQLHQGSPEQIAQMLLTGTVDIGIASHLLAERPELVA
                     LPCYRWTHSIVVPEGHPLVADGEVTLERLAKYPIITYDIGLTGRWSIDAAFKRAGLRP
                     DIVITAMDADIIKTYVDLGLGVGILASIAMDEDQPGRLRAVDARHLFSINTTSVAVRR
                     GGLLRGYAYDFIQTFASHLTREVVEAAQAAPSDTEQAPL"
     gene            complement(2663..3028)
                     /locus_tag="VIN75_18755"
     CDS             complement(2663..3028)
                     /locus_tag="VIN75_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEY8976262.1"
                     /translation="MLTTIANVFVALIALLHVYILVLEMFLWTTPRGRKAFGTTAEFA
                     EQSKVLAANQGLYNGFLAAGLAWGLCMGAEGHGIKLFFLGCVLVAGIYGGVTANKRIL
                     FIQAVPAAIGIALVLAAGA"
BASE COUNT          493 a         1086 c         1136 g          486 t
ORIGIN      
        1 tcggcttgaa cctcggcctg cccctggggg gcgccacggt gggcgtgcat gcgctgaccg
       61 ggcgcatcgg cagcccggtg gacggccagt cgctcacggc catgcggatc cttggcggct
      121 acgccgtcta tgacgaggac aactgggagg ccatggccga atacttcggc ttcgtcgatc
      181 gcaacgtcgt cggcggcggc ctgcacacca gccacgccgc ctatgcccag gtggggcgca
      241 gcttcggtgc cgtgacgccc tacgcgcgct acgaactcgc ctcgctcgac cagggcgacg
      301 actacttcgc gtcgatgatc ggcgggcggt cgtaccggcg cgcgctggtg ggcgcgcgct
      361 tcgacatcga cccgcgttcc accctgaagc tggagttcga ccacacgagc ctgaccgacc
      421 ggatcaagga tcgctaccgc gagtcgcgtg tcgaatacgc gatccgcttc tgagacgtcg
      481 atggcaggca aggcgcattt cctcgtcctg ggcgtcgcgg cgctgcttgc aggagccgcg
      541 ccgggtgctg cgcgcgcaca ggccgtcatc acgaacaccg gcgtcacgat cgtcgcggcg
      601 gacgtgcgca tgatctacat cggcgagaag cagttcacgg gggcggtgcg actgatcccg
      661 gtggacaaca gcgccgtcca ggaccggttc ctggccgcct tcctccaggt cgacaacggc
      721 aagtactact cgatctgggc caagaagagc ttccgcgaag gcgtgaaccc gccgctcctg
      781 aaggcgaccg acggcgaggt cgtcgacttc gtgcggcgca cgcccggggc catcggctac
      841 atcggcgacc cgtccaaggc ggtgggcgtc accatcgtca aatgaacgcg aaccgcctcg
      901 cccgccgctc ggtcctgggc accctggcgg ccctggcgtt gcccgcatcg gccgtggcgc
      961 cgcaggcgac ggccaatgtc tggagcctgc acggccagtg gaccgacgac cagggccaca
     1021 gcgtcgcgat ggccgactgg gccggccggc cggcgctgct gacgctggcc tacgggtctt
     1081 gccggcgcat ctgcagcacg agcctgcgcc tgctcgagcg ggcgcagcag gaggccgacg
     1141 cgcgcaaggt cgacctctcg gtgatcgtcg tcagcctcga cccgcgcgtg gacacgcccg
     1201 cgtcctggcg agagtaccgg gccgagcacc ggctgcagcg cgcgaactgg catttcctcg
     1261 acggcacgcc ggccctgacc cggcgtgtag ccgacctgct cgagatgcag tactggatct
     1321 acgacgacca cgtgctgcac gacttgcgca tcgccgcggt cgatgtctcg gggcggatcg
     1381 tcgcgcgcat cgactgggtc gacgagccgc cgcggcggct catcgatgcc ctggcgtcct
     1441 gatccctaca gcggcgcctg ctcggtgtcg ctcggcgcgg cctgcgccgc ctcgaccacc
     1501 tcgcgcgtca ggtgcgacgc gaaggtctgg atgaagtcgt acgcatagcc gcgcagcagg
     1561 ccgccgcggc gcacggccac gctggtcgtg ttgatcgaga acaggtggcg cgcatcgacg
     1621 gcccgcaggc ggccgggctg gtcctcgtcc atcgcgatcg aggccaggat gcccacgccc
     1681 aggccgaggt cgacgtaggt cttgatgatg tcggcgtcca tcgcggtgat gacgatgtca
     1741 ggccgcaggc cggcgcgctt gaaggcggcg tcgatgctcc agcggccggt caggccgatg
     1801 tcgtaggtga tgatcgggta cttggccagc cgctcgagcg tgacctcgcc gtcggccacc
     1861 agcgggtggc cctcgggcac gacgatggag tgcgtccagc ggtagcacgg cagcgcgacc
     1921 agctcggggc gctcggccag caggtgggac gcgatgccga tgtcgacggt tcccgtcagc
     1981 agcatctgcg cgatctgctc gggcgagccc tggtgcagtt gcagctggac gcccgggtgg
     2041 gcggcgcgga agtcgcgcac ggccgtcggc agcgcgtagc gcgcctgcga gtgggtggcg
     2101 gcgatcgtga gcacgccggt cgtgccctgg gtgaattcct cgctggcgcg ctgcaggctc
     2161 tcggcgtcct gcagcaggcg ttccacccac ggcagcacgg ccgcgccggg ctgggtcagg
     2221 cccgtcaggc gcttgcccac gcgcacgaag atctcgacgc ccagctcttc ctcgagctcg
     2281 cggatctggc ggctgacgcc cggctgggac gtgtgcaacg cctgggcgac ctcggtcagg
     2341 ttgaagccgc ggcggacggc ttcgcgcacg gagcgcagtt gctggaaatt catggtgggc
     2401 tcgaaatcgt tatctgacaa acgattatcg agccccattc gatatgcgta agttcgttat
     2461 tacgcagctc catgcgcgga aaacggatat gcccgcgcag cctcacaaga agagaaggcg
     2521 tcatcccccg cgcagtcgca aggatccacg gtggccggca gtttcggctc gccaaggcgg
     2581 atcctgcgac tgcgcgcagg atgacggcgc gtggagagcc gggcacggcg gctcatcagg
     2641 cgacccgggc ggaccttgct cctcacgccc ccgcggccag caccagcgca atgccgatcg
     2701 ctgcgggcac cgcctggatg aacaggatcc gcttgttggc cgtgacgccg ccgtagatgc
     2761 ccgccaccag cacgcatccc aggaagaaca gcttgatgcc gtgcccctcg gcgcccatgc
     2821 aaaggcccca ggcgaggccg gcggccagga agccgttgta caggccctgg ttggcggcca
     2881 gcaccttgct ctgctcggcg aattcggccg tggtgccgaa cgccttgcgc ccgcgcggcg
     2941 tcgtccacag gaacatctcc agcacgagga tgtagacatg cagcagcgcg atcaaggcga
     3001 cgaagacgtt ggcgatcgtg gtgagcatga ggggatcctt gtggctgagt tcggcgcgga
     3061 gcatgccacg tcttggcctg tgcgcaatgc gccaaggtga cacggcgggt tcgccgaccg
     3121 gcgtcatggc cgggggcgga tcctgcgact gcgcgcagga tgacggcgcg tggagagccg
     3181 ggcacggcgg ctcatcaggc g
//
LOCUS       DATCRO010000693        13781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_952210, whole genome shotgun sequence.
ACCESSION   DATCRO010000693 DATCRO010000000
VERSION     DATCRO010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952210"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VIN75_18760"
     CDS             complement(<1..266)
                     /locus_tag="VIN75_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8976263.1"
                     /translation="MTRRAATSPADDGALDRTLQALAEPTRRAVVGLLRHGPQRAGEI
                     ADALAMSRQAMSRHLRVLRQAGVIHEVGAAGPDDDARARTYRLEA"
     gene            complement(263..736)
                     /locus_tag="VIN75_18765"
     CDS             complement(263..736)
                     /locus_tag="VIN75_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8976264.1"
                     /translation="MRKSPPAGWPQAQSGLYYRDAAKAIDWLCDAFGFEVRLKVEGEP
                     GQIIHSELVYGSAMIYVGSAAKAGEAAEPGREFKTRHASPLDVGGRNTQSVCLFVDDA
                     DAHCAQARAHGAVISSEPATHDYGDDYWSDRSYGAYDLEGHSWWFMHRVREQPKT"
     gene            857..3475
                     /locus_tag="VIN75_18770"
     CDS             857..3475
                     /locus_tag="VIN75_18770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY8976265.1"
                     /translation="MRTSLSRLAAGVLVLLCTGAIAAPVNRMILPDDVVPSAYRLDIT
                     PNMAESTFDGRVEIDVDVRRRTNRIELDSADLAISTASLDGEAAAPRMALDARRQRAT
                     FTFAHALAPGRHVLRIAYSGHINDQAAGLFHLDHDAPQGPVRSLYTMFEDSDARRFVP
                     SWDEPARRATFELTVTVPAALMAVGNMPVASTEALPGELKRVRFEKTPPMSTYLLFFG
                     LGDFERVHRDVGGVDVGVVVKRGDTAQAGYALDAAAKLLPYYNAWFGTPYPLPKLDMV
                     AGAGGSVTFGAMENWGAIFYFERDLLVDDRRASEDDRRRVFETVAHEIAHQWFGNLVT
                     MAWWDDLWLNEGFASWMQVKAVEQLHPEWTGWANDAGDRQRAMDIDARRGTHPVIMHI
                     DDVSQAEGAFDTITYSKGAQVVRTLEAWQGDDTFRDGVRRYIARHAYGNTVTEDLWRA
                     LDVDTPRPVAAIARDLTLQAGVPLVTELASRCTDGRTVVTLSQGQFSIDAPPVARWHV
                     PVKVAVVGGGGTRAVVTGAKPQDVVVPGCGPVIINAGQSTYARVRYSPADLAALMPAF
                     GRLGVDDQLGLLADTQALALAGRLPMGELLALMAGVPAARDKPAVAQLLVAQVEDLDR
                     LHDGLASQPAFRAWARALLAPFLAEVGWDGRADDSGDLRTLRVALIEALGEFGDPQVL
                     GRVEPAFERYMARHDSLDADHREGVLYVVATRADERTWEQLHGLALAAGSELEKAEGY
                     EMLGHARDPALARRALELAISGEPPTTVASRLMRAVGRVHPGPTLDFAAGHWAQVEPL
                     FGEGAGATIAARFFDTGADPALLPRLDAFVRAHVPAATRARIVKNAALIRYRATVRTQ
                     RLPEADRWIAAHPPAA"
     gene            3639..4589
                     /locus_tag="VIN75_18775"
     CDS             3639..4589
                     /locus_tag="VIN75_18775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976266.1"
                     /translation="MSTSASSSAGGVTAAERAQIQRANISGLQPVVFVHGLWLLPESW
                     ASWAEAFEEAGYIALTPGWPDDPASVAEANANPGVMAGKTVGQVADHLSTVISQLERK
                     PAVIGHSFGGLLAQILAGRGQSHVTVAIAPAPFRGVLPLPLSALKASWPVLGSLSNRR
                     RAIGLTYEQFRFAFANAVSEDEARALFAEHAVPAPGAPLFQAATANLNPWTEAQVDTL
                     NQERGPLLVVSGQKDNTVPWAMANAAYRQQLLNPGHTGIVEIPGRGHSLVIDSGWREV
                     CDAALAFVRRFAAPDAYDSAYLDYVDTPIREDEDRRVTVF"
     gene            complement(4586..5179)
                     /locus_tag="VIN75_18780"
     CDS             complement(4586..5179)
                     /locus_tag="VIN75_18780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8976267.1"
                     /translation="MDKTTLAAYDADPAGYARSWDTQPPPDDMYEQLRTWFTPGPTAE
                     IGTGSGRDAAWLAARGFDVHGFDASAGLLAQARAAHPGIPFDHATLPELAGVARGAWQ
                     NVLCETVIMHLDVAQIAPSVRTLLALLRPGGTLYLSWRVTPDASTRDPAGRLYAAFDK
                     SLVTSALAPGDEIVFDEDATSVASGKRLQRLVIRRAS"
     gene            complement(5184..5696)
                     /locus_tag="VIN75_18785"
     CDS             complement(5184..5696)
                     /locus_tag="VIN75_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976268.1"
                     /translation="MKLVMDVHFAGETARVGAVAFEDWGAAEGTRQYALDIAHVDKPA
                     KGELDLRALPWLIRVLEANGLQPEAIVLDGFVHLDAQDTPALGRRLHDALAGRSAVIG
                     VSKSVFKGVDTPDQFCVFREDETPPLVVTCAGIDLGAAKARVRMMHGRKRVPTLMKLA
                     ARIAKGTVDA"
     gene            complement(5720..6091)
                     /locus_tag="VIN75_18790"
     CDS             complement(5720..6091)
                     /locus_tag="VIN75_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976269.1"
                     /translation="MDQREFEAVVDEFGADVQDLVELVETARYEQATAVEISRLLDAR
                     EDLLVLVNKLPALRAAEPPPAMNVPLASLRARIAHAREALKSSHDSREILQMVAETGE
                     TFAGIVAPLQAAGRWPKGRSR"
     gene            complement(6201..6809)
                     /locus_tag="VIN75_18795"
     CDS             complement(6201..6809)
                     /locus_tag="VIN75_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEY8976270.1"
                     /translation="MPCYEIDGLRPVVHPTAYVHPTAVLIGDVVVGPGAYIGPCASLR
                     GDFGRIVLQDGCNVQDTCVIHGFPASDTLLEDNAHIGHGAVLHGCVVRADALVGMNAV
                     VMDEAEIGAGAFVAASAFVRAGLKVPPRSLAAGVPAKVLRELSDQELGWKREGTATYQ
                     RLAERCLATMKECAPLAAEEPGRKRLDAGTVQPLVATRRAAD"
     gene            7044..7862
                     /locus_tag="VIN75_18800"
     CDS             7044..7862
                     /locus_tag="VIN75_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8976271.1"
                     /translation="MSEPTLLSSPDALILVAQRGAVRTLTLNRPAALNSFTADMHAEL
                     RAALDAAAADDAVRAILITGAGRGFCAGQDLNDPGMAAGADGKPDIAAVIEKNYRPLA
                     LRIQSMPVPVVCAVNGVAAGAGANFAFCCDIVLAARSASFIQAFAKIGLIPDCGGTWL
                     LPRLAGRPRALALALTGDKLSAADAERFGMVWQVVDDAELLAQAQALADKLAAMPSKA
                     LAATRQALDASQALTLSDALTLEAHMQGELGRAHDFAEGVAAFQQKRAPVFKDR"
     gene            7862..8293
                     /gene="paaI"
                     /locus_tag="VIN75_18805"
     CDS             7862..8293
                     /gene="paaI"
                     /locus_tag="VIN75_18805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEY8976272.1"
                     /translation="MSEAQKIAERVRDHMWSTDRASQGMGMRVAAIGPGSCTMTMTVR
                     EDMLNGYGTCQGGLLAALADSCFAFACNAYNEITVAAGFDVQIVAPGKLGDQLVAIAT
                     EVSKTGRLGVYDVDVRNQRDERVLAFRGRSYTMKGKPVLAE"
     gene            8410..9717
                     /gene="paaK"
                     /locus_tag="VIN75_18810"
     CDS             8410..9717
                     /gene="paaK"
                     /locus_tag="VIN75_18810"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEY8976273.1"
                     /translation="MPIHAVRPVELDPIERASRDEIAALQLARLRATLEHVYANVPHY
                     KRAFDEKGVHPSDLKTLADLAKFPFTGKADLRANYPFGMFAVPREQVVRVHASSGTTG
                     KPTVVGYTQQDIDHWADLVARSLRAAGVRKGDMVHVAYGYGLFTGGLGAHYGAERLGC
                     TVVPMSGGQTEKQVQLITDFKPDAIMVTPSYMQVICEEFRRLGLDPREMSVKVGIFGA
                     EPWTEAMRRDIQQAANIDAVDIYGLSEVMGPGVASECIETQDGPVIWEDHFYPEIIDP
                     DTGEVLPDGQEGELVFTTLTKQAFPVIRYRTRDLTRLLPPTARSMRRMGKIVGRSDDM
                     LIIRGVNLFPTQIEEIVLAHGALGGQYQLVVTRDGLLDKVEVLCELLPAHAGADPGAV
                     GRELQERIKTLTGVSTAVRVGPPDSIERTLVGKARRVVDQRKK"
     gene            9906..10919
                     /gene="paaA"
                     /locus_tag="VIN75_18815"
     CDS             9906..10919
                     /gene="paaA"
                     /locus_tag="VIN75_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY8976274.1"
                     /translation="MYTQAMDTAPKEERVAIRNADEVVAEEAFERALDNEGFIEAKDW
                     MPDHYRKTLVRQISQHAHSEIVGMLPEGNWITRAPTLKRKAILLAKVQDEAGHGLYLY
                     AAGETLGTSRDQMLDALHSGKAKYSSIFNYPTLTWADVGVIGWLVDGAAIMNQIPLCR
                     CSYAPYARAMVRVCREESFHQRQGYDALLTMMQKGTDAQRAMVQDATNRWWWPSLMMF
                     GPPDDQSPNTAQSMRWGIKRLTNDELRQKFVNATVEQARILGVSLPDPDLKWNEARNG
                     YDFGAIDWTEFWQVVNGDGPCNKERLAERVDAWNDGAWVREAALAHARKQAARAAEGR
                     KAA"
     gene            11096..11362
                     /gene="paaB"
                     /locus_tag="VIN75_18820"
     CDS             11096..11362
                     /gene="paaB"
                     /locus_tag="VIN75_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY8976275.1"
                     /translation="MWEVFVRPKAGLDHKHCGSLHAADSKMALQLAREVYTRRQEGVS
                     LWIVRSDQIVASDPADKAMLFDPMEDKVYRHPTFYELPPEVGHM"
     gene            11481..12212
                     /gene="paaC"
                     /locus_tag="VIN75_18825"
     CDS             11481..12212
                     /gene="paaC"
                     /locus_tag="VIN75_18825"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY8976276.1"
                     /translation="MRIGDSCLIHAQRLAEWSGHAPILEEDIALTNLALDLVGQARAL
                     LTHVGRQTGFDEDQLAFLRGERDFFNFTLTELPGRRAGRDFADAILRNFLLSAWMNVL
                     WAKLEESGDAEVAAIAGKAVKESRYHVAHAADWVVRLGDGSEESARRMRQAVDRLWPY
                     AAELFETDAVDTAAAEAGLGPVRAALRVGWQAIVEPVFADAGLKLPATGAFRSTGSRG
                     VHSEHMGYLLAEMQTLQRSFPGGVW"
     gene            12206..12742
                     /gene="paaD"
                     /locus_tag="VIN75_18830"
     CDS             12206..12742
                     /gene="paaD"
                     /locus_tag="VIN75_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY8976277.1"
                     /translation="MVSAMAVRAPASCARVTRAWEVLHGVLDPEVPAISVTDLGIVRD
                     VVDHGDALEVVVTPTYSGCPATEVIEQSIAAAIDEAGLGPARVTQRRAPAWTTDWITP
                     EGREKLRAYGIAPPGSCAGEATASSVQPIRLMARDVACPRCGSAQTERLSAFGSTVCK
                     SLYRCIACREPFEHFKPI"
     gene            12756..>13781
                     /locus_tag="VIN75_18835"
     CDS             12756..>13781
                     /locus_tag="VIN75_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8976278.1"
                     /translation="MAVDTHFHPLRVRSVRPETDEAMVVSFDIPESLSDRFRFTQGQY
                     LTLRQRIAGEEVRRSYSICAGIDDGELRVGVRRVRGGRFSNWLAENLRAGDTIDVMPP
                     QGRFFVPIDASVARNYLCVAGGSGITPMISIMRTVLANEPRSRVTLVYGNRRAASTMF
                     KEELEDLKNRYVARVSLHHVFSEERVDSPLHQGMLDRAKLGEFLQGLIRADHIDHAFV
                     CGPFMMNDEAEAALLAAGVAAERIHIERFGVPIPADSGFGAQAAPVPAPAPGDAADAQ
                     LTVVRDGVTHQIEFHAADNNLLDACAHAGLDVPYSCKSGVCGTCRAKCVDGEVRMERN
                     FALDKNEV"
BASE COUNT         2053 a         4941 c         4856 g         1931 t
ORIGIN      
        1 gcctccagcc ggtaggtgcg ggcgcgggcg tcgtcgtcgg ggccggcggc gcccacctcg
       61 tggatgacgc cggcctggcg caggacgcgc aggtggcggc tcatcgcctg gcgcgacatc
      121 gcgagcgcgt cggcgatctc gcccgcgcgc tgcgggccgt ggcgcagcag ccccacgacc
      181 gcgcgccgcg tgggctcggc cagcgcctgc agcgtgcggt cgagcgcgcc gtcgtcggcc
      241 ggggaggtgg cggcgcggcg cgtcacgtct tcggctgctc gcgcacgcgg tgcatgaacc
      301 accacgaatg gccctcgagg tcgtacgcgc cgtagctgcg gtcgctccag tagtcgtcgc
      361 catagtcgtg cgtggcaggt tccgacgaga tgaccgcgcc gtgcgcgcgg gcctgcgcgc
      421 agtgcgcgtc ggcatcgtcg acgaacaggc acaccgactg cgtgttgcgg ccgccgacgt
      481 ccagcggact ggcgtgccgg gtcttgaact cgcggccggg ctcggcggcc tcgcccgctt
      541 tcgccgcgct gccgacgtag atcatcgcgc tgccatagac gagctcgctg tggatgatct
      601 ggccgggctc gccctcgacc ttcagtcgca cctcgaagcc gaacgcgtcg cagagccaat
      661 cgatggcctt ggcggcgtcg cggtagtaga ggccggactg ggcctgcggc cagccggccg
      721 gcggggactt gcgcatggga tgcctcgctg gaaatggtca acgaatggat tgatggtaaa
      781 ccgaatcgtt aatcaaatgc cggaagaccc cggcccgatc cctagaatgc gcgacgctgc
      841 ttcctacccc gtttccatgc gaacctccct ttcccggctc gccgccggcg tcctcgtcct
      901 gctgtgcacc ggcgcgatcg ccgcgcccgt gaaccgcatg atcctgcccg acgacgtcgt
      961 gccgtccgcg taccgcctcg acattacgcc gaacatggcc gagtccacgt tcgacggccg
     1021 cgtcgagatc gacgtcgacg tgcgccgccg cacgaaccgc atcgagctgg acagcgccga
     1081 cctggccatc tccaccgcga gcctggacgg cgaggccgcc gcgccgcgca tggcgctcga
     1141 cgcaaggcgc cagcgcgcca cgttcacctt cgcgcacgcg ctcgcgccgg gccgccacgt
     1201 gctgcgcatc gcctactcgg gccacatcaa cgaccaggcc gccggcctgt tccacctcga
     1261 ccacgacgcg ccgcaaggcc ccgtgcgctc gctgtacacg atgttcgagg actccgacgc
     1321 gcgccgcttc gttccgagct gggacgaacc ggcgcgccgc gccaccttcg agctgaccgt
     1381 gaccgtgccg gccgcgctga tggccgtcgg caacatgccc gtcgcgtcca ccgaggcgct
     1441 gcccggcgag ctcaagcgcg tgcggttcga gaagacgccg ccgatgtcga cctacctgct
     1501 gttcttcggc ctgggcgact tcgagcgcgt gcaccgcgat gtcggcggcg tcgacgtggg
     1561 cgtcgtcgtc aagcgcggcg acaccgcgca ggccggatac gcactggacg ccgcggcgaa
     1621 gctgctgccg tactacaacg cgtggttcgg cacgccgtac ccgctgccca agctggacat
     1681 ggtggccggc gcgggcggca gcgtcacctt cggcgcgatg gagaactggg gcgcgatctt
     1741 ctacttcgag cgcgacctgc tcgtcgacga ccggcgcgcc agcgaggacg accgccgccg
     1801 cgtgttcgag accgtggcgc acgagatcgc gcaccagtgg ttcggcaacc tggtcacgat
     1861 ggcctggtgg gacgacctgt ggctcaacga gggtttcgct tcgtggatgc aggtcaaggc
     1921 cgtcgagcag ctgcaccccg aatggacggg ctgggccaac gacgccggcg acaggcagcg
     1981 cgcgatggac atcgacgcgc gccgcggcac gcacccggtc atcatgcaca tcgacgacgt
     2041 gtcgcaggcc gagggcgcgt tcgacacgat cacctactcg aagggcgcgc aggtggtgcg
     2101 cacgctggag gcctggcagg gcgacgacac cttccgcgac ggcgtgcggc gctacatcgc
     2161 caggcacgcg tacggcaaca cggtgaccga ggacctgtgg cgcgcgctgg acgtcgacac
     2221 gccgcgcccc gtcgccgcca tcgcgcgcga cctgacgctg caggccggcg tgccgctggt
     2281 caccgagctg gcttcgcgct gcacggacgg ccgcacggtg gtcacgctgt cgcaaggcca
     2341 gttcagcatc gatgcgccgc ccgtcgcgcg ctggcacgtg ccggtgaagg tggccgtcgt
     2401 gggcggcggc gggacgcgcg ccgtcgtcac gggcgcgaag ccgcaggatg tcgtggtgcc
     2461 cggctgcggc ccggtgatca tcaacgcggg ccagtcgacg tacgcccgcg tccgctactc
     2521 gcctgccgac ctggccgcgc tgatgcccgc gttcggacgc ctgggcgtcg acgaccagct
     2581 gggcctgctg gccgacacgc aggcgctcgc gctggccggc cggctgccga tgggcgagct
     2641 gctcgcgctg atggccggcg tgccggccgc gcgcgacaag cccgccgtcg cgcagctgct
     2701 ggtggcgcag gtggaggatc tggatcggct gcacgacgga ctggcctcgc agccggcctt
     2761 ccgcgcctgg gcgcgggcgc tgctcgcgcc gttcctggcc gaggtcggct gggacggccg
     2821 cgcggacgac agcggcgacc tgcggacgct gcgcgtcgcg ctcatcgagg cgctcggcga
     2881 gttcggcgac ccgcaggtgc tgggccgggt cgaaccggcg ttcgagcgct acatggcgcg
     2941 gcacgacagc ctcgacgccg atcaccgcga aggcgtcctg tacgtcgtcg cgacgcgggc
     3001 ggacgaacgc acctgggagc agttgcacgg gctggcgctg gcggccgggt cggagctgga
     3061 aaaggccgaa ggctacgaga tgctgggcca cgcgcgcgat ccggcgctgg cgcggcgcgc
     3121 gctcgaactg gcgatctccg gcgagccgcc gacgacggtg gccagccgcc tcatgcgcgc
     3181 ggtcggacgc gtgcacccgg gcccgacgct ggacttcgcc gccggccact gggcgcaggt
     3241 cgagccgctg ttcggcgagg gcgcgggcgc cacgatcgcc gcgcgcttct tcgacaccgg
     3301 cgccgatccg gcgctgctgc cgcgtctgga cgcgttcgtg cgcgcccacg tcccggcggc
     3361 cacgcgcgcg cgcatcgtca agaacgccgc gctgatccgc taccgtgcca cggtgcgcac
     3421 gcagcgcctg cccgaggccg atcgctggat cgcggcgcat ccgccggcag cctgaccggc
     3481 tgaccggtcg tcgcgtcgga cacacgcgat gtaacggacc tcctcggaac aggggacgtt
     3541 cgcactcccc cgattgacgt cgcgcaacat ttggatacga tgaaatcgga tccgaaacgg
     3601 actcgcgtcg aggcccggat ccggcgggga gcaggcgcat gagcacttcg gcatcgtcat
     3661 ccgcgggcgg cgtcacggcc gccgaacgcg cgcagatcca gcgtgccaac atctcgggac
     3721 tgcagcccgt cgtcttcgtg cacggcctgt ggctgctgcc cgaaagctgg gcttcctggg
     3781 ccgaggcctt cgaggaggca ggctacatcg ccctgacccc cggctggccc gacgaccccg
     3841 ccagcgtggc cgaggccaac gccaaccccg gcgtgatggc cggcaagacg gtgggccagg
     3901 tggccgacca cctctccacc gtcatctccc agctcgaacg caagccggcc gtcatcggcc
     3961 attcgttcgg cggcctgctg gcgcagatcc tcgccggccg cggccagtcg cacgtcaccg
     4021 tggccatcgc ccccgcgcca ttccgcggcg tgctgccgct gccgctgtcg gcgctcaagg
     4081 cgtcgtggcc ggtgctgggc agcctgtcga accggcgccg cgcgatcggc ctgacctacg
     4141 agcagttccg cttcgcgttc gccaacgccg tgagcgagga cgaggcgcgc gccctgttcg
     4201 ccgagcacgc ggtgccggcc ccgggcgcgc cgctgttcca ggccgcgacg gccaacctca
     4261 atccgtggac cgaggcgcag gtcgacacgc tgaaccagga gcgcgggccg ctgctggtcg
     4321 tctccggcca gaaggacaac acggtgccgt gggcgatggc caacgccgct taccgtcagc
     4381 agctgctcaa tccgggccac accgggatcg tcgagatccc cggccgcgga cactcgctgg
     4441 tcatcgacag cggctggcgc gaggtctgcg acgcggcgct cgccttcgtg cgccgcttcg
     4501 ccgcgccgga cgcgtacgac tcggcctacc tcgactacgt cgacacgccc atccgcgagg
     4561 acgaggaccg tcgggtcacc gtcttctagg acgcgcgccg gatcacgagc cgctgaaggc
     4621 gcttgcccga cgcgacgctg gtggcgtcct cgtcgaagac gatctcgtcg ccgggcgcca
     4681 gcgcgctcgt caccagcgac ttgtcgaacg cggcgtagag ccggccggcc gggtcgcgcg
     4741 tggacgcgtc cggcgtcacg cgccagctca ggtaaagcgt cccgccgggg cgcagcagcg
     4801 ccagcagcgt gcgcaccgac ggcgcgatct gcgcgacgtc caggtgcatg atcaccgtct
     4861 cgcacagcac gttctgccac gcgccgcgcg ccacgccggc cagctcgggc agcgtggcgt
     4921 ggtcgaacgg gatgcccgga tgcgcggcgc gcgcctgggc cagcaggccg gccgacgcgt
     4981 cgaatccgtg cacgtcgaag ccgcgggcgg cgagccacgc ggcgtcgcgg ccgctgcccg
     5041 tgccgatctc ggcggtcggg ccgggcgtga accaggtgcg caactgctcg tacatgtcgt
     5101 cgggcggcgg ctgggtgtcc cagctgcggg cgtagccggc cgggtcggcg tcgtaggcgg
     5161 caagggtggt cttgtccatg tgctcaggcg tcgacggtgc ccttggcgat gcgcgccgcc
     5221 agcttcatca gcgtgggcac gcgcttgcgc ccgtgcatca tgcgcacgcg cgccttggcg
     5281 gcgcccaggt cgatgccggc gcaggtgacg accagcggcg gcgtctcgtc ctcgcggaag
     5341 acgcagaact ggtcgggcgt gtccacgccc ttgaagacgg atttcgacac cccgatgacg
     5401 gcgctgcgcc cggccagggc gtcgtgcagg cggcggccga gggcgggcgt gtcctgggcg
     5461 tccaggtgca cgaagccgtc gagcacgatg gcctcgggct gcaggccgtt ggcctcgagc
     5521 acccggatca gccagggcag cgcgcgcagg tcgagttcgc ccttggcggg cttgtcgacg
     5581 tgcgcgatgt ccagcgcgta ctggcgggtg ccctcggcgg cgccccagtc ctcgaatgcg
     5641 accgcgccga cccgggcggt ctcgcccgcg aagtggacgt ccatgacgag tttcatggac
     5701 gcattgtgac cgcagcgggt cagcgcgagc ggcccttggg ccagcggccc gcggcctgca
     5761 gcggcgcgac gatgccggcg aaggtctcgc cggtctcggc caccatctgc aggatctccc
     5821 tggagtcgtg gctgctcttg agcgcctcgc gcgcgtgggc gatgcgggcg cgcaggctcg
     5881 cgagcggcac gttcatggcg ggcggcggct cggccgcgcg cagggccggc agcttgttga
     5941 ccagcaccag caggtcctcc cgcgcatcga gcagccgcga gatctccacg gccgtcgcct
     6001 gctcgtagcg cgccgtctcc accagctcga ccaggtcctg cacgtcggct ccgaactcgt
     6061 cgacaaccgc ctcgaactcg cgttgatcca ttcttgtgtc tcccgggtga agggctggat
     6121 tttgcaagcc acggaggatg cgccaacccc atgaaccgtc gcaagcgtac gcgaaaagtc
     6181 gaggcagcgt tgccgtcgat ctagtcggcc gcgcgcctgg tcgccaccag cggttggacg
     6241 gtgccggcat ccaggcgctt tcgccccggc tcctccgcgg ccagcggcgc gcattccttc
     6301 atcgtggcca ggcaccgttc ggccaggcgc tggtaggtgg cggtgccctc gcgcttccag
     6361 ccgagttcct ggtcgctgag ctcgcgcagc accttggccg gcacgccggc ggccagcgag
     6421 cggggcggca ccttgaggcc ggcgcgcacg aaggcgctgg cggcgacgaa cgcgccggcg
     6481 ccgatctcgg cctcgtccat cacgaccgcg ttcatgccca ccagcgcgtc ggcgcggacg
     6541 acgcagccgt gcagcacggc cccgtggccg atgtgggcgt tgtcctccag cagggtgtcg
     6601 gacgccggga aaccgtggat cacgcaggtg tcctgcacgt tgcagccgtc ctgcaggacg
     6661 atgcggccga agtcgccgcg caggctggcg caggggccga tgtaggcgcc tgggcccacc
     6721 acgacgtcac cgatgagcac ggcggtgggg tggacatagg ccgtcgggtg gacgaccggg
     6781 cgcaggccgt cgatctcgta gcagggcatt cgggtttacc tcaatcatgg cagtgcggac
     6841 cgtgcgggct tgcgccggat caagggtgag tctaagatta accgaccggt cggtcaaaga
     6901 atcgaccaag ccagcgatgc tggtggggga gacaaccctt ttcgagcgat ggcggacgcg
     6961 ggcgaccccg cgcggccgcg tcgcatcccg gcgcccgccc aatcccagat ccggggtttg
     7021 cagcaaccct tccgaggcca gccatgtccg aaccgactct cctgtcgtcg cccgatgcgt
     7081 tgatcctggt ggcgcagcgc ggcgccgtgc gcacgctcac gctgaaccgt ccggcggcgc
     7141 tcaacagctt caccgccgac atgcacgccg agctgcgcgc ggcgctcgac gccgcggccg
     7201 ccgacgacgc cgtgcgcgcc atcctgatca ccggcgcggg ccgcggcttc tgcgcggggc
     7261 aggacctgaa cgaccccggc atggccgccg gcgccgacgg caagcctgac atcgccgccg
     7321 tcatcgagaa gaactatcgc ccgctcgcgc tgcgcatcca gtcgatgccg gtgccggtgg
     7381 tgtgcgccgt caacggcgtg gccgcgggcg cgggcgcgaa cttcgcgttc tgctgcgaca
     7441 tcgtgctggc cgcgcgctcg gccagcttca tccaggcctt cgccaagatc ggcctgatcc
     7501 ccgactgcgg cggcacctgg ctgctgccgc gcctggcggg ccgcccgcgc gcgctcgcgc
     7561 tggcgctcac gggggacaag ctctcggccg ccgacgccga gcgcttcggc atggtctggc
     7621 aggtggtcga cgacgccgag ctgctggccc aggcgcaggc gctggccgac aagctggccg
     7681 cgatgccgtc caaggcgctg gcggccacgc gccaggcgct cgacgcctcg caggcgctga
     7741 cgctgtcgga cgcgctgacg ctggaggccc acatgcaggg cgaactgggc cgcgcgcacg
     7801 acttcgcgga gggtgtggcg gcgttccagc agaagcgcgc gcccgtcttc aaggatcgct
     7861 gatgtccgag gcgcagaaga tcgccgagcg cgtgcgcgac cacatgtggt ccaccgatcg
     7921 cgcctcgcag ggcatgggca tgcgggtcgc ggccatcggg cccggcagct gcacgatgac
     7981 gatgacggtg cgcgaggaca tgctcaacgg ctacggcacc tgccagggcg gcctgctggc
     8041 cgcgctggcc gactcctgct tcgcgttcgc gtgcaacgcc tacaacgaga tcaccgtcgc
     8101 cgccggcttc gacgtgcaga tcgtcgcacc gggcaagctg ggcgaccagc tcgtggccat
     8161 cgccaccgag gtgagcaaga ccggccgcct gggcgtctac gacgtcgacg tgcgcaacca
     8221 gcgcgacgag cgcgtgctcg cgttccgcgg ccgttcgtac accatgaaag gcaagccggt
     8281 tcttgccgaa tgacatgacg acccccggct cacttcgtac gcagcccccc gagggggcgc
     8341 gcgggctcct ccggagcggc cgagcgcacg cccgcgctcg agtcagagct ttgctttcca
     8401 aggactgaga tgcccatcca tgccgtgcgc cccgtcgaac tcgaccccat cgaacgcgcc
     8461 agccgcgacg agatcgccgc gctgcagctg gcgcgcctgc gcgccacgct ggaacacgtc
     8521 tacgccaacg tgccgcacta caagcgggcg ttcgacgaga agggcgtgca cccgtcggat
     8581 ctcaagacgc tggccgacct ggcgaagttc ccgttcaccg gcaaggccga cctgcgcgcc
     8641 aactacccgt tcgggatgtt cgcggtgccg cgcgagcagg tggtgcgcgt gcacgcgtcc
     8701 tcgggcacga cgggcaagcc caccgtcgtc ggctacacgc agcaggacat cgaccactgg
     8761 gccgacctcg tcgcgcgctc gctgcgcgcc gcgggcgtgc gcaagggcga catggtgcac
     8821 gtggcctacg gctacggcct gttcaccggc ggcctgggcg cgcactacgg cgccgagcgg
     8881 ctgggctgca cggtggtgcc gatgtccggc ggccagaccg agaagcaggt gcagctgatc
     8941 accgacttca agcccgacgc gatcatggtc acgccgtcgt acatgcaggt catctgcgag
     9001 gagttccgcc gcttggggct ggatccgcgc gagatgagcg tgaaggtcgg catcttcggc
     9061 gccgagccgt ggaccgaggc gatgcggcgc gacatccagc aggcggcgaa catcgacgcc
     9121 gtcgacatct acggcctgtc ggaggtgatg ggcccgggcg tggccagcga atgcatcgag
     9181 acgcaggatg gcccggtgat ctgggaagac catttctacc cggagatcat cgaccccgac
     9241 acgggcgagg tgctgcccga cgggcaggag ggcgagctcg tcttcacgac gctgaccaag
     9301 caggccttcc cggtcatccg ctaccgcacg cgcgacctca cgcgcctgct gccgccgacc
     9361 gcgcgcagca tgcgccgcat gggcaagatc gtcggccgct ccgacgacat gctgatcatc
     9421 cgcggcgtca acctgttccc gacgcagatc gaggagatcg tgctggcgca cggcgctctc
     9481 ggcggccagt accagctggt ggtcacgcgc gacgggctgc tggacaaggt cgaggtgctg
     9541 tgcgagctgc tgcccgctca cgcgggcgcc gacccgggcg ccgtcggccg cgagctgcag
     9601 gaacgcatca agacgctgac cggcgtgagc accgcggtgc gcgtcgggcc gcccgattcg
     9661 atcgaacgca ctttggttgg aaaggctcgt cgagtcgtcg accagcgcaa gaaatgaatc
     9721 ccgtatcagg tactgaatgt cgcgcgccga gacatcggcg tcggggtcag gtcttgaaag
     9781 ttgcgcgacg atggatcggc catcggctga ggcatcaacg cgcaactttc aagacctgac
     9841 cccatgggaa gcgcagcaag cgcaacattc aagacctgac cccgtccatt tgagaggatc
     9901 acgccatgta cacccaagcc atggacaccg cgcccaagga agagcgcgtc gccatccgca
     9961 acgccgacga ggtcgtcgcc gaggaggcct tcgagcgcgc cctcgacaac gagggcttca
    10021 tcgaggccaa ggactggatg cccgaccact accgcaagac gctggtgcgc cagatcagcc
    10081 agcacgcgca ctcggagatc gtgggcatgc tgcccgaggg caactggatc acgcgcgcgc
    10141 cgaccctcaa gcgcaaggcg atcctcctgg ccaaggtgca ggacgaggcc ggccacggcc
    10201 tgtacctgta cgcggcgggc gagacgctgg gcacctcgcg cgaccagatg ctggacgcgc
    10261 tgcattcggg caaggccaag tactcgtcga tcttcaacta tccgacgctc acgtgggccg
    10321 acgtcggcgt gatcggctgg ctggtggacg gcgcggccat catgaaccag atcccgctgt
    10381 gccgctgctc gtacgcgccg tatgcccgcg cgatggtgcg tgtgtgccgc gaggaatcct
    10441 tccaccagcg ccagggctac gacgccttgc tgacgatgat gcagaagggc accgacgcgc
    10501 agcgcgcgat ggtgcaggac gccaccaacc gctggtggtg gcccagcctg atgatgttcg
    10561 gcccgccgga cgaccagtcg cccaacaccg cgcagtcgat gcgctggggc atcaagcggc
    10621 tgaccaacga cgaactgcgc cagaagttcg tcaacgccac ggtcgaacag gccaggatcc
    10681 tgggcgtctc gctgccggac cccgacctga agtggaacga ggcgcgcaac ggctacgact
    10741 tcggcgccat cgactggacc gagttctggc aggtcgtcaa cggcgacggc ccgtgcaaca
    10801 aggaacgcct ggccgagcgc gtggatgcgt ggaacgacgg cgcctgggtg cgcgaggccg
    10861 cgctggcgca cgcccgcaag caggccgccc gcgccgccga aggccggaaa gccgcgtgac
    10921 ccgacgaaca ttgcaagaga gcaacgacat gaccgcatcc cctaccgatc tcgccaatgc
    10981 caacgccaag gcccctggcc cggacgagcg cacggccgct ccgaagccgg ccgggcccag
    11041 ccccgcgggg gcgacgagca aagcgagtgg ggccgtcact tcgcatgaat ggccgctgtg
    11101 ggaggtgttc gtgcgcccca aggccggcct cgaccacaag cactgcggca gcctgcacgc
    11161 cgccgactcg aagatggcgc tgcagctggc gcgcgaggtc tacacgcggc gccaggaagg
    11221 cgtcagcctg tggatcgtgc gctcggacca gatcgtcgcg tccgacccgg ccgacaaggc
    11281 gatgctgttc gatccgatgg aggacaaggt ctatcgccat ccgacgttct acgagctgcc
    11341 gcctgaagtc ggccacatgt gagggcgcca tgacgactac cttcgccccc ttgccgacga
    11401 ccgcgagcgc ctcgaacgcc gctgcatcga ccaccaccga gtccatcgtc ttccggccca
    11461 cgccgcagca gcagtacctg atgcgcatcg gcgacagctg cctgatccac gcgcagcgcc
    11521 tggccgaatg gagcggtcac gcgccgatcc tggaagagga catcgcgctg accaacctcg
    11581 cgctcgacct ggtcggccag gcgcgcgcac tgctgacgca cgtcggccgc cagacgggct
    11641 tcgacgagga ccagctcgcc ttcctgcgcg gcgagcgcga cttcttcaat ttcacgctca
    11701 ccgagctgcc gggccgccgc gcgggccgcg acttcgccga tgcgatcctg cgcaatttcc
    11761 tgctgtccgc gtggatgaac gtgctgtggg ccaagctcga ggagtcgggc gacgccgagg
    11821 tggccgccat cgccggcaag gctgtcaagg aatcgcgcta ccacgtggcc catgcggccg
    11881 actgggtggt gcgcctgggc gacggcagcg aggagtcggc gcgccgcatg cgccaggcgg
    11941 tcgatcgcct gtggccctac gccgcggagc tgttcgagac ggacgccgtc gacacggccg
    12001 ccgccgaggc cggcctcggt ccggtccgcg cggcgctgcg cgtcggctgg caggccatcg
    12061 tcgagcccgt gttcgccgat gccggcctga agctgcccgc cacgggcgcg ttccgttcca
    12121 ccggctcgcg cggcgtgcac agcgagcaca tgggctacct gctggccgag atgcagacac
    12181 tgcagcgctc gttcccgggc ggcgtatggt gagcgcgatg gccgtgcgcg cgccggcctc
    12241 ctgcgcccgc gtcacgcgcg cgtgggaggt gctgcacggc gtgctcgatc ccgaggtgcc
    12301 ggcgatctcg gtcaccgacc tgggcatcgt gcgcgacgtg gtcgaccacg gcgacgcgct
    12361 cgaggtggtg gtcacgccga cgtattcggg ctgccccgcg accgaggtca tcgagcagtc
    12421 gatcgccgcc gcgatcgacg aggccggcct cgggcccgcg cgcgtcacgc agcggcgcgc
    12481 gcccgcgtgg acgaccgact ggatcacgcc ggaaggccgc gagaagctgc gcgcctacgg
    12541 catcgcgccg ccggggtcgt gcgccggcga ggcgaccgct tcgagcgtgc agcccattcg
    12601 cctgatggcg cgcgacgtcg cctgtccgcg ctgcggcagc gcgcagaccg agcgcctgtc
    12661 ggccttcggc tcgacggtgt gcaagtcgct gtaccgctgc atcgcctgcc gcgaaccctt
    12721 cgaacacttc aagccgatct gagcaaagac aagccatggc cgtcgacacc cacttccacc
    12781 cgttgagagt gcgcagcgtg cgccccgaga ccgacgaggc gatggtcgtc tcgttcgaca
    12841 tccccgagag cctgagcgac cgcttccgct tcacgcaggg ccagtacctg acgctgcgcc
    12901 agcgcatcgc cggcgaggag gtgcgccgct cgtactcgat ctgcgccggc atcgacgatg
    12961 gcgaactgcg cgtgggcgtg cggcgcgtgc gcggcggccg cttctcgaac tggctggccg
    13021 agaacctgcg cgcgggcgac accatcgacg tcatgccccc gcagggccgc ttcttcgtgc
    13081 ccatcgacgc gagcgtggcg cgcaactacc tgtgcgtggc gggcggcagc ggcatcacgc
    13141 cgatgatctc catcatgcgc acggtgctgg ccaacgagcc gcgcagccgc gtgacgctgg
    13201 tctacggcaa ccgccgcgcg gcctcgacga tgttcaagga agaactggag gacctcaaga
    13261 accgctacgt cgcccgggtc tcgctgcacc acgtgttctc cgaggagcgc gtggactcgc
    13321 cgctgcacca gggcatgctc gaccgcgcca agctgggcga gttcctgcag ggcctgatcc
    13381 gcgccgacca catcgaccac gccttcgtct gcggcccgtt catgatgaac gacgaggccg
    13441 aggccgcgct gctggccgcc ggcgtggccg ccgagcgcat ccacatcgag cgctttggcg
    13501 tgcccatccc cgccgactcg ggctttggcg cgcaggccgc gccggtgccc gcgccggcgc
    13561 ccggcgacgc ggccgacgcg cagctgaccg tcgtgcgcga cggcgtcacg caccagatcg
    13621 agttccacgc ggccgacaac aacctgctcg atgcctgcgc ccacgcgggc ctggacgtgc
    13681 cctactcgtg caagtcgggc gtgtgcggaa catgccgcgc gaagtgcgtg gacggggaag
    13741 tccgcatgga aagaaacttc gcgctcgaca agaatgaagt c
//
LOCUS       DATCRO010000694         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_300266, whole genome shotgun sequence.
ACCESSION   DATCRO010000694 DATCRO010000000
VERSION     DATCRO010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300266"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VIN75_18840"
     CDS             complement(<1..1120)
                     /locus_tag="VIN75_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8976279.1"
                     /translation="MATIEVKVPDIGDFKDVGVIELLVKPGDTVKAEQSLITVESDKA
                     SMEIPSSTAGVVRELRVKVGDKVNMGDVILVVDGEGAAASPAPAPAPAPANASPAPAQ
                     APAPAPVSAHAGPVPVASTYGGGADIECDVLVLGGGPGGYSAAFRAADLGMKVVLVER
                     YPTLGGVCLNVGCIPSKALLHVAAVMDEVAHFADLGVSYGAPQIDVGKLRTHKGKVVG
                     KLTGGLAAMAKMRKVTVVRGYGNFLDPFHVKVDETSGEGQDKTGNAKTIKFKNAIIAA
                     GSQAVRLPFFPNDPRIVDSTGALELRGTPKKMLIVGGGIIGLEMGTVYSTLGARLDVV
                     EMLDGLMQGADRDLVKVWQKMTAKRFDNILLKTKTVGAE"
     gene            complement(1135..2826)
                     /gene="aceF"
                     /locus_tag="VIN75_18845"
     CDS             complement(1135..2826)
                     /gene="aceF"
                     /locus_tag="VIN75_18845"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEY8976280.1"
                     /translation="MAMVEVKVPDIGDFKDVAIIEVLVKPGDSVKAEQSLLTVESDKA
                     SMEIPSSAAGVVKEMKVAMGDKVNEGTVILLLETGAAAAPAPAPAPAQAAAPAPAPAP
                     VQGPAPAPAGAGGLVDVEVPDIGDFADVGVIEILVKVGDTVKVEQSLITVESDKASME
                     IPSSHAGVVKALKVKVGDRVSKGSVVATIEGAGGAVAPAAAPAQTVASPKPAPAEEAS
                     DSGTSAAAEIQDPRGIEKPLPASAMPSHDPTKPPVNLPHASPSIRRIARELGVPLEEV
                     KGSGPHGRITQPDLEGFVKAVMAGATQTKAQAAKAPAAAAGGALPGLLPWPQVDFAKF
                     GTVERKDLSRIKKISGANLHRNWVMIPHVTNHDDADITDLEAFRVTLNKEYEKSGTKV
                     TMLAFLIKACVAALKKFPEFNASLDGDTLVLKKYFHIGFAADTPNGLMVPVIRDADKK
                     GIVQIAKEMSELAAKARDGKLGPADMTGGCFSISSLGGIGGTYFTPIINAPEVAILGV
                     CKSATRPVWDGKAFVPRLILPLSLSWDHRVIDGAAAARFNTYLGQVLADFRRVML"
     gene            complement(2851..>3543)
                     /gene="aceE"
                     /locus_tag="VIN75_18850"
     CDS             complement(2851..>3543)
                     /gene="aceE"
                     /locus_tag="VIN75_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703183.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8976281.1"
                     /translation="DPTFAHEVAVIMQDGLKRMVENQENVYYYITLLNENYAMPGLQP
                     GTEQQIIKGMYLLQPAGPGKLTVNLLGSGTILRESMAAKELLEAEWGVGANVWSCPSF
                     NELARDGQAAERHNLLHPTDAPKKSFVEQQLAPHDGPVVASTDYIRSYAEQIRAFIPR
                     GRAYKVLGTDGYGRSDFRSKLREHFEINRHYIVVASLKALADEGKLPLAKVAEAIKKY
                     GIDADKINPLYA"
BASE COUNT          472 a         1247 c         1208 g          616 t
ORIGIN      
        1 cctcggcgcc caccgtcttc gtcttcagca ggatgttgtc gaagcgcttg gcggtcatct
       61 tctgccagac cttgacgagg tcgcggtcgg cgccctgcat caggccgtcc agcatctcga
      121 cgacatccag gcgcgcgccc agcgtggagt acaccgtgcc catctcgaga ccgatgatgc
      181 cgccgccgac gatcagcatc ttcttcgggg tgccgcgcag ctccagcgcg cccgtcgaat
      241 cgacgatgcg cgggtcgttc gggaagaacg gcaggcgcac ggcctgcgaa cccgccgcga
      301 tgatcgcgtt cttgaacttg atcgtcttgg cattgccggt cttgtcctgg ccctcgccgc
      361 tggtctcgtc gaccttgacg tggaacgggt ccaggaaatt cccgtagccg cgcacgaccg
      421 tcaccttgcg catcttggcc atcgccgcca ggccgcccgt gagcttgccc acgaccttgc
      481 ccttgtgcgt gcgcagcttg ccgacgtcga tctgcggcgc gccgtacgac acgcccaggt
      541 ccgcgaagtg cgcgacctcg tccatcaccg ccgccacgtg cagcaacgcc ttggacggga
      601 tgcagcccac gttcaggcag acgccgccga gcgtcggata gcgctcgacc agcaccacct
      661 tcatgcccag gtcggccgca cggaacgccg ccgaatagcc gccggggccg ccgccgagca
      721 cgagcacgtc gcactcgatg tcggcgccgc cgccatacgt ggacgcgacg ggcaccggcc
      781 ccgcgtgcgc gctcacgggc gccggcgcgg gcgcttgcgc cggcgcgggt gacgcgtttg
      841 cgggcgcggg cgcgggggcc ggcgcgggag acgccgcggc gccctcgccg tcgaccacca
      901 ggatgacgtc gcccatgttg accttgtcgc ccaccttgac gcgcagttcc ctgaccacgc
      961 ccgcggtcga cgacgggatc tccatcgacg ccttgtcgct ctcgacggtg atcaggctct
     1021 gctcggcctt gacggtgtcg ccgggcttga ccagcagctc gatgacgccg acgtccttga
     1081 aatcgccgat gtcgggaacc ttgacttcga tcgttgccat gtcgcccccg ccccttacag
     1141 catcacccgg cggaagtccg cgaggacctg gccgaggtag gtgttgaagc gcgcggccgc
     1201 cgccccgtcg atgacgcggt gatcccacga cagcgacagc ggcaggatca ggcggggcac
     1261 gaaggccttg ccgtcccaca ccggcctggt cgcgctcttg cagacgccca ggatggcgac
     1321 ttcgggcgcg ttgatgatcg gcgtgaagta cgtgccgccg atgccgccca gcgagctgat
     1381 cgagaagcag ccgccggtca tgtcggccgg gcccagcttg ccgtcgcgcg ccttggccgc
     1441 gagctcgctc atctccttgg cgatctgcac gatgcccttc ttgtcggcgt cgcggatcac
     1501 cgggaccatc aggccgttgg gcgtgtccgc cgcaaagccg atgtggaagt acttcttgag
     1561 caccagcgtg tcgccgtcca gcgaggcgtt gaactcgggg aacttcttga gcgccgcgac
     1621 gcaggccttg atgaggaacg cgagcatcgt caccttggtg cccgacttct cgtattcctt
     1681 gttcagcgtc acgcggaacg cctccaggtc ggtgatgtcc gcgtcgtcgt gattggtgac
     1741 gtgcgggatc atgacccagt tgcgatgcag gttcgcgccc gagatcttct tgatgcgcga
     1801 caggtccttg cgttcgacgg tgccgaactt ggcgaagtcg acctgcggcc acggcagcag
     1861 gcccggcagc gcgccacccg cggcggcggc gggcgccttc gcggcctggg ccttggtctg
     1921 cgtggcgcct gccatcacgg ccttgacgaa gccctcgagg tcgggctgcg tgatgcggcc
     1981 gtgcgggccg gagcccttca cctcctccag cggcacgccc agttcgcggg cgatgcggcg
     2041 gatcgacggc gacgcgtgcg gcaggttgac cggcggcttg gtcgggtcgt gcgacggcat
     2101 ggccgacgcc ggcagcggct tctcgatccc gcgcggatcc tggatctcgg cggcggcgct
     2161 ggtgcccgag tcggacgcct cttcagccgg cgccggcttg ggcgacgcga ccgtctgcgc
     2221 cggcgcggcc gcgggcgcga cggcgccccc cgcgccctcg atcgtggcga cgaccgagcc
     2281 cttggacacg cggtcgccga ccttgacctt cagcgccttg acgacgccgg cgtgcgacga
     2341 cgggatctcc atcgaggcct tgtcgctctc gacggtgatc aggctctgct cgaccttcac
     2401 ggtgtcgccg accttgacga ggatctcgat gacgcccacg tcggcgaagt cgccgatgtc
     2461 gggcacctcc acgtcgacca ggccaccggc gccggccggc gcgggcgccg ggccctgcac
     2521 gggggcgggc gcgggcgcgg gcgcggccgc ctgggcgggc gccggggccg gcgcgggcgc
     2581 tgcggcggcg ccggtctcga gcagcaggat caccgtgccc tcgttgacct tgtcgcccat
     2641 cgcgaccttc atctccttga cgacgcccgc cgcggacgac gggatctcca tcgaggcctt
     2701 gtcgctctcg acggtcagca ggctctgctc cgccttgacg ctgtcgcccg gcttgaccag
     2761 cacctcgatg atcgcgacgt ccttgaagtc gccgatgtcg ggcaccttca cttcaaccat
     2821 tgccatgttg ttgtctccgt gaaggtgtga tcaggcgtac agcgggttga tcttgtcggc
     2881 atcgatgccg tacttcttga tggcctcggc caccttggcc agcggcagct tgccctcgtc
     2941 ggccagcgcc ttcagcgacg ccacgacgat gtagtggcgg ttgatctcga agtgctcgcg
     3001 cagcttgctg cggaagtccg agcggccgta gccgtccgtg cccagcacct tgtaggcgcg
     3061 gccgcgcggg atgaaggcgc ggatctgctc ggcgtagctg cggatgtagt cggtcgacgc
     3121 caccaccggg ccgtcgtgcg gcgcaagctg ctgctcgacg aaggacttct tcggcgcgtc
     3181 ggtcgggtgc agcaggttgt ggcgctcggc ggcctggccg tcgcgcgcca gctcgttgaa
     3241 gctcgggcag ctccacacgt tggcgcccac gccccactcg gcctccagca gttccttggc
     3301 ggccatggac tcgcgcagga tcgtgcccga gcccagcagg ttgacggtca gcttgccggg
     3361 gcccgcgggc tgcagcaggt acatgccctt gatgatctgc tgctcggtgc ccggctgcag
     3421 gccgggcatc gcgtagttct cgttcaggag cgtgatgtag tagtacacgt tctcctggtt
     3481 ctcgaccatg cgcttgaggc cgtcctgcat gatgaccgcc acctcgtgcg cgaaggtcgg
     3541 gtc
//
LOCUS       DATCRO010000695         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1031576, whole genome shotgun sequence.
ACCESSION   DATCRO010000695 DATCRO010000000
VERSION     DATCRO010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031576"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIN75_18855"
     CDS             complement(<1..195)
                     /locus_tag="VIN75_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8976282.1"
                     /translation="MSVSSGFIKRPIGTTLLALALLLVGIAVFPLLPIAPLPQVDFPT
                     IQVSANLPGASPETMASNVAQ"
     gene            complement(192..1376)
                     /locus_tag="VIN75_18860"
     CDS             complement(192..1376)
                     /locus_tag="VIN75_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976283.1"
                     /translation="MSTHFSRHRTTWALVAIALAVLALGLWSIERPANAADKGKGKRG
                     DEGPVAITSTLVKQQDVPIYRTGIGTVTPTYSVTVKARVDGQLDRVAFTEGQDVRKGQ
                     LLAQLDPRTLQAQLLQAQAAKAKDQATLANARVDLKRYETLIAQDAATQQQLDTQKAL
                     VNQLDAGVKADEAAVNAAQVQLGFTTITAPISGRVGARLVDPGNIVHAADTTGLVVIN
                     QIDPISVVFTLPDDAVQETIHALDSNKPVLVQAFPRDGGNMLGAGYLTLLNNQIDTST
                     GTVQLKASFINGQHNLWPGQYVNVRLILGTRSKALTVPEQAVQRSQSGVYVYVVDEQT
                     GTVQSRTIALTQIQDGIAVISSGVNANDRVVVDGQYKLKPGSKVMEAAKAASGTASGA
                     RK"
     gene            complement(1388..2827)
                     /locus_tag="VIN75_18865"
     CDS             complement(1388..2827)
                     /locus_tag="VIN75_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8976284.1"
                     /translation="MSLRLSLLALAAAALLSACAAGPDYVRPALPVPDTYKEAGDWKP
                     ADPRPAASGLAWWTAYGDPVLDGLEADAAKANQNIRQAEAAYRQAKALADADRAGLFP
                     SLGLNAGAQRARTNTNGVRTGDAYALGLAASWEPDLWGSVRRQVEAGNAGAQASADDL
                     AAARLSIQTTLAQDYVQLRIADQQRDLYAATVEAYGKALKLTRAQHDAGVALQSDVAL
                     AEAQLAQAQAAAVDLEATRAQLEHAIAVLTGRAPAQFTLAAAPAGQPLALKTPAAPVG
                     LPSALLEHRPDIAAAERRAAAANAQIGVAKAAYFPQLTLSANGGFSAAALDSLFDTPA
                     HVWSLGAALAQTLFDGGLRSARTAQAEAAYDAAAAQYKQTVLGGFQQVEDQLALLRLL
                     DQEATLQDQAVRSSQQAERLALAQYRAGTASYLGVVTAQTLSLNNQRSAVQLRGRLVA
                     ASIALVAATGGGWSATDSIAVAATPAQPQ"
BASE COUNT          343 a          981 c         1102 g          442 t
ORIGIN      
        1 ctgcgccacg ttcgacgcca tcgtctccgg gctggcgccc gggaggttgg ccgagacctg
       61 gatggtgggg aagtcgacct gcggcagcgg cgcgatgggc agcagcggga acaccgcgat
      121 gcccaccagc agcagcgcca gggcgagcag cgtggtgccg atcggccgct tgatgaaacc
      181 ggacgagacg ctcacttgcg ggctcccgag gccgtgcccg aggcggcctt ggcggcctcc
      241 atcaccttgg agccgggctt gagcttgtac tggccgtcga cgacgacgcg gtcgttggcg
      301 ttgacgcccg acgagatcac cgcgatcccg tcctggatct gcgtcagcgc gatcgtgcgg
      361 ctctgcaccg tcccggtctg ctcgtcgacc acgtagacgt acacgccgct ctggctgcgc
      421 tgcaccgcct gctcgggcac cgtcagcgcc ttcgagcgcg tgcccaggat caggcgcacg
      481 ttgacgtact ggcccggcca caggttgtgc tggccgttga tgaagctggc cttgagctgc
      541 acggtgccgg tcgacgtgtc gatctggttg ttcagcagcg tgagatagcc cgcgcccagc
      601 atgttgccgc cgtcgcgcgg gaaggcctgc accagcacgg gcttgttcga atcgagcgcg
      661 tggatcgtct cctgcaccgc gtcgtcgggc agcgtgaaga cgaccgagat cgggtcgatc
      721 tggttgatca cgaccagccc cgtggtgtcg gccgcgtgga cgatgttgcc cgggtcgacc
      781 aggcgcgcgc cgacgcggcc gctgatcggc gcggtgatcg tcgtgaagcc cagctgcacc
      841 tgcgccgcgt tgacggccgc ctcgtcggcc ttgacgccgg cgtcgagctg gttgaccagc
      901 gccttctgcg tgtcgagctg ctgctgcgtg gcggcgtcct gcgcgatcag cgtctcgtag
      961 cgcttgaggt cgacgcgcgc attggcgagc gtggcctggt ccttggcctt ggcggcctgc
     1021 gcctgcagca gctgcgcctg cagcgtgcgc gggtcgagct gggccagcag ctggcctttc
     1081 ctgacgtcct ggccttcggt gaaggcgacc ctgtccagct ggccgtcgac gcgcgccttg
     1141 acggtgaccg agtacgtcgg cgtcacggtg ccgatgccgg tgcgatagat cggcacgtcc
     1201 tgctgcttga ccagcgtgct cgtgatggcg acgggccctt cgtcgccgcg cttgcccttg
     1261 cccttgtcgg cggcattggc cggacgttcg atcgaccaca ggccgagcgc gagcacggcg
     1321 agcgcgatgg ccacgagggc ccaggtggtg cggtgacgag agaaatgggt gctcatggca
     1381 ggtggcgtca ttgcggctgc gcgggggtgg ccgcgacagc gatggaatcg gtggcgctcc
     1441 agccgccgcc ggtggcggcc acgagcgcga tgctggccgc gacgaggcgg ccacgcagct
     1501 ggacggcgct gcgctggttg ttcagcgaca gcgtctgagc cgtcaccacg cccaggtagc
     1561 tggccgtgcc ggcgcggtac tgcgcgagcg cgagccgctc ggcctgctgc gacgagcgca
     1621 cggcctggtc ctgcagcgtg gcttcctggt cgagcaggcg cagcagcgcc agctggtcct
     1681 cgacctgctg gaaaccgccc agcaccgtct gcttgtactg cgcggcggcc gcgtcgtacg
     1741 ccgcctcggc ctgcgcggtg cgggcgctgc gcaggccgcc gtcgaacagc gtctgcgcca
     1801 atgcggcgcc gagcgaccag acgtgcgccg gcgtgtcgaa caggctgtcg agcgcggccg
     1861 cgctgaagcc gccgttggcg ctgagcgtca gctgcgggaa gtacgcggcc ttggcgacgc
     1921 cgatctgcgc attggcggcg gcggcgcggc gctcggcggc ggcgatgtcg gggcggtgtt
     1981 cgagcagcgc cgacggcagg ccgaccggcg cagcgggcgt cttgagcgcg agcggctggc
     2041 cggcgggcgc cgcggccagc gtgaactgcg ccggggcgcg gccggtgagc acggcgatcg
     2101 cgtgctccag ctgagcgcgc gtggcctcga ggtcgaccgc cgcggcctgc gcctgcgcga
     2161 gctgcgcctc ggcgagcgcg acatccgact gaagtgcgac gcccgcgtcg tgctgcgcgc
     2221 gcgtgagctt gagcgccttg ccgtaggcct cgacggtggc ggcgtacagg tcgcgctgct
     2281 ggtcggcgat gcgcagttgc acgtagtcct gcgccagcgt cgtctggatg ctcaggcgcg
     2341 cggcggccag gtcgtcggcg ctcgcctgcg cgcccgcgtt gccggcctcg acctggcgcc
     2401 gcacgctgcc ccacaggtcg ggctcccagc tcgccgccag gcccagcgcg taggcgtcgc
     2461 cggtgcgcac gccattggtg ttggtgcgcg cgcgctgggc gccggcgttc aggcccagcg
     2521 acgggaacag cccggcgcgg tcggcgtcgg ccagcgcctt ggcctggcgg tacgcggcct
     2581 ccgcctggcg gatgttctgg ttggccttcg cggcatcggc ctccaggccg tccagcaccg
     2641 gatcgccata cgccgtccac caggccagcc cgctggcggc cgggcgtggg tcggcgggct
     2701 tccagtcgcc ggcttccttg taggtgtcgg gcaccggcag cgcgggccgc acgtagtcgg
     2761 gcccggcggc gcaggccgac agcagcgcgg cggccgccag ggcaagcagg gagaggcgca
     2821 gggacatggc cgggaggact tcgggagtga gcgggacagg ctgcactc
//
LOCUS       DATCRO010000696         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_480578, whole genome shotgun sequence.
ACCESSION   DATCRO010000696 DATCRO010000000
VERSION     DATCRO010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480578"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIN75_18870"
     CDS             <1..237
                     /locus_tag="VIN75_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976285.1"
                     /translation="LECQPGYADQLAASLERQPTELGAWMANRLLNSRLPRDERGAWL
                     KRLTVVTSHPKAAPGVRDSAIRFLDFQASRRADS"
     gene            complement(234..626)
                     /locus_tag="VIN75_18875"
     CDS             complement(234..626)
                     /locus_tag="VIN75_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976286.1"
                     /translation="MNHLNPFQAFASAMPSFTQPILPGWTLNIDSNNSSSPRTEGLVV
                     SRFSYGRQLGQVNDALAAIVALLPAEARALPEVERFMRMKACIDVLKDDSLDDRVASL
                     AADLARLKDSKDSKAFDRATAPLRELLP"
     gene            complement(741..1154)
                     /locus_tag="VIN75_18880"
     CDS             complement(741..1154)
                     /locus_tag="VIN75_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976287.1"
                     /translation="MALAFGVRAAGVLRGRAPVDEALLAPDAAARRGRARRIFTWAVA
                     GEALGIVLAVNLAVNLGHPLWQVPAAMAVIGLHFLPLASGYHYRPHLFTGIALTAWAL
                     VFPWLFAAGPVAPAGMLGAAAILYASAAGSLHSAR"
     gene            1156..1389
                     /locus_tag="VIN75_18885"
     CDS             1156..1389
                     /locus_tag="VIN75_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976288.1"
                     /translation="MASSPQAHAGAPDLSNPAPAHTLAKPLTTSEPVARPRIRKLSHA
                     SSLRVGKFNILGMAVEVMQVSSRDVDAGQSGRG"
     gene            2047..2325
                     /locus_tag="VIN75_18890"
     CDS             2047..2325
                     /locus_tag="VIN75_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY8976289.1"
                     /translation="MKATWNGAVLAESDDIVMVEGNHYFPESSLNRDYITFSNHRTSC
                     PWKGQAYYYTLLVNGQMNPEAVWYYPEPSDAAANIKGRVAFWKGVKVE"
     gene            2370..>3225
                     /locus_tag="VIN75_18895"
     CDS             2370..>3225
                     /locus_tag="VIN75_18895"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8976290.1"
                     /translation="MRPIHKKLFAALAAAGLLAVSAFAEDAAPPKVGDSPASSAAAAV
                     TAASAPAAVTPLAHLPYTPVLDVTSMDKAADPCVDFFQYACGGWQKNNPIPADQSRWD
                     VYSKMEQDNRRVLWGILDRLAAQTTGRTVSQQKIGDAFAACTNEELTERLGAGPMKPE
                     LDLIDGMRTKKDLPAVLARLQLDSGDDGLFFSFGSAQDYADSSRVIAFATQGGLGLPE
                     RDYYFKTDARSKELREKYVAHVARMFQLMGDSQADAKAHADVVMSMETALAKGSMTAV
                     ELRDPQKLF"
BASE COUNT          556 a         1169 c         1066 g          434 t
ORIGIN      
        1 ctcgaatgcc agcccgggta tgccgaccag ctcgcggcgt cgctcgaacg gcagccgacg
       61 gaactcggcg cctggatggc caaccgcctg ctcaactcga ggctgccgcg cgacgagcgc
      121 ggcgcctggc tgaagcggct gaccgtcgtc acatcgcacc cgaaggcggc ccccggcgtg
      181 cgcgattcgg cgatccgctt cctggacttc caggcgagcc gccgcgcgga ctcctaaggt
      241 agcaactcgc gcagcggcgc ggtggcgcgg tcgaaggcct tgctgtcctt cgaatccttg
      301 agcctggcca ggtccgcggc cagcgacgcg acgcggtcgt ccaggctgtc gtccttgagc
      361 acgtcgatgc aggccttcat gcgcatgaag cgttcgacct ccggcagcgc gcgcgcctcg
      421 gcgggcagca gcgcgacgat cgccgccagc gcgtcgttga cctgcccgag ctggcgcccg
      481 tagctgaatc gggagacgac caggccctcg gtgcgcggcg acgacgagtt gttgctgtcg
      541 atgttgagcg tccatcccgg caggatgggt tgcgtgaacg acggcatcgc gctcgcgaag
      601 gcctggaacg ggttcaggtg gttcatgcgt gtgctccctg tgcgtgacgg ttcggtcggc
      661 gcgaggcgcc gtggccgcag ctgcgattct cggagcaagc gcgcgcgcgg cgtaccccac
      721 gtccgcagcg cgcgccgcgt tcagcgtgcc gaatgcaggc tgcccgccgc gctggcgtac
      781 aggatcgcgg ccgcgcccag catgccggcc ggggccaccg gcccggccgc gaacagccag
      841 ggaaagacca gcgcccaggc ggtcagcgcg atgccggtga acaggtgcgg gcggtagtgg
      901 tagccggagg cgagcggcag gaagtgcagg ccgatgaccg ccatcgccgc gggcacctgc
      961 cacaacggat ggcccaggtt cacggccagg ttgaccgcca gcacgatgcc cagcgcctcg
     1021 ccggccacgg cccacgtgaa gatgcgcctg gcgcgaccgc ggcgcgccgc cgcgtccggc
     1081 gcgagcaggg cttcgtcgac cggcgcacgg ccgcgcagca cgcccgccgc gcgcacgccg
     1141 aatgccaggg ccacgatggc cagcagcccc caggcccacg ccggcgcgcc ggacctcagc
     1201 aatccggcgc ccgcccacac gctcgcgaag ccgctgacga ccagcgagcc ggtcgcgcgt
     1261 ccgcggatcc gcaaactttc gcacgcgtcg tcgctgcgcg tgggcaagtt caacattttg
     1321 gggatggccg tcgaggtcat gcaggtctcc agtagagatg tcgatgcagg gcagtctggc
     1381 cgcggatgac gccccggtct gccttgctgc gacggatggc gagcgacgcc gacggatcga
     1441 cgaacgcatg tgtaaatgca atcaccgatg tgcaccgccg cgtacgctgc tacgttgtag
     1501 agacaggcac cgcaacaggt gtcgccgcga gggcctccac gtttgcaccc agaggcgttt
     1561 cctggcgcgc cgattgctag tacctgatga cacagatgaa agccgaagga gggcaacatc
     1621 atgaaagtcg caggcgaaac cgaacctgaa ccttggcgac cgctgggaga acgcgtcacg
     1681 cgcagcccgc cagccaagcc cgagtggcag ccgttgcctc atgccccgtg catcgaggtc
     1741 aaccgccagg gccagttgcg aaccaacctg ccgctgccga agtaattcgc gcacggccac
     1801 ccaaggcgag gcctgatcaa cccgcctgag atcccgaccg gcattgcccg gtcgacccgc
     1861 cggctccgcc aagacggagc gccccaacgg cgacacgacg ttccgagcgg acgtcaaggc
     1921 ggagcttccc tcccctatac aagatgcccg gcgcgggaag ccccgaggcg tcgcccctct
     1981 cccgacctgc gaaactagcg gccttgcacg cgcgcgcgtc cgccgcgtcc cacgggagaa
     2041 cacctcatga aagccacctg gaatggcgcc gtcctggccg agagcgacga catcgtcatg
     2101 gtcgagggca accactactt tcccgaatcc agcctgaacc gggactacat caccttcagc
     2161 aaccaccgca cgagctgccc gtggaagggc caggcgtact actacacgct gctggtcaac
     2221 ggccagatga atcccgaagc ggtctggtac taccccgaac cctccgacgc cgcggccaac
     2281 atcaagggcc gcgtcgcgtt ctggaagggc gtgaaggtcg agtgaccctc gccgagcacg
     2341 gtccaaaaga caaccactgc caccacccca tgcgacccat ccacaagaag ttgttcgccg
     2401 cgctcgcggc cgccggcctg ctggccgtct ccgccttcgc agaggacgcc gcgccgccca
     2461 aggtcggcga ctcgcccgcg tcgtcggccg ctgccgccgt caccgccgcc tcggcgcccg
     2521 ccgcggtcac cccgctggcg cacctgccct acacgccggt gctggacgtc acgtcgatgg
     2581 acaaggcggc cgacccgtgc gtcgacttct tccagtacgc ctgtggcggc tggcagaaga
     2641 acaacccgat cccggccgac cagtcgcgct gggacgtcta ctccaagatg gagcaggaca
     2701 accgccgcgt gctgtggggc atcctcgacc gcctggccgc ccagaccacc ggacgcaccg
     2761 tttcgcagca gaagatcggc gacgccttcg ccgcgtgcac caacgaggag ctcacggagc
     2821 gtctcggtgc gggcccgatg aagcccgagc tggacctgat cgacggcatg cgcaccaaga
     2881 aggacctgcc cgcggtgctg gccaggctgc agctggactc cggcgacgac gggctgttct
     2941 tcagcttcgg ttcggcccag gactacgccg actcgtcgcg cgtgatcgcc ttcgccaccc
     3001 agggcggcct gggcctgccc gagcgcgact actacttcaa gaccgacgcc aggtccaagg
     3061 aactgcgcga gaagtacgtg gcccacgtgg cccgcatgtt ccagctgatg ggcgacagcc
     3121 aggccgacgc caaggcgcac gccgacgtcg tcatgtccat ggagacggcg ctcgccaagg
     3181 gctcgatgac ggccgtggag ctgcgcgacc cgcagaagct gttcc
//
LOCUS       DATCRO010000697         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_446303, whole genome shotgun sequence.
ACCESSION   DATCRO010000697 DATCRO010000000
VERSION     DATCRO010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446303"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VIN75_18900"
     CDS             <1..546
                     /locus_tag="VIN75_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976291.1"
                     /translation="PAKKAVAKKPVAKKAEPAAATNDHLSPGQLAAAEHVLTGVAECE
                     MKEKVDIEKIAGHDGNFKVTYNHKTYTMVPEETTTGAVRLVDLTGAVVWIQIPMKSML
                     MNQKEHHRLVDGCEEAEQRSAVAAQQQSTNAAATAAAAQTAAPGSTITTTTTTTTQVV
                     APAPVKNAAAAPTTAASGMWK"
     gene            complement(903..>2178)
                     /locus_tag="VIN75_18905"
     CDS             complement(903..>2178)
                     /locus_tag="VIN75_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8976292.1"
                     /translation="DENEGAVEQSDSSLVRLINSMIVEAHNQGVSDIHIETRPGREKV
                     KVRFRVDGLLRPYLELPHTYRSALVARLKIMCDLDISERRRPQDGKINFARFVPQCPI
                     ELRVAMIPTANGLEDAVLRILASAKPLPLDSLGMSPHNLTHLKRAIERPYGMVLCVGP
                     TGSGKTTTLHSALNFINTPERKIWTAEDPVEITQAGLRQVQVNPKIDWTFAKALRAFL
                     RADPDVIMVGEIRDNETAQIAVESSLTGHLVLSTLHTNSAPETITRLLDMGMDPFNFA
                     DALLAVLAQRLVRRICSGCKTATPATDEQVEELLNDTLHVWGDHPQAPSRDDLLAGWT
                     SRFAVDGRLQFYRGTGCAKCGNTGVKGRAGVHELLTVSKGVRHLIQQGARAEQIQMHA
                     MGEGMRTLRQDGIEKVLAGVTTIEEVRATSNS"
BASE COUNT          371 a          733 c          741 g          333 t
ORIGIN      
        1 cccgccaaga aggccgtcgc caagaagccg gtcgccaaga aggccgagcc cgctgcggcc
       61 acgaacgacc acctgagccc gggccagctg gccgctgctg aacacgtcct gaccggcgtg
      121 gccgagtgcg agatgaagga gaaggtcgac atcgagaaga tcgccggcca tgacggcaac
      181 ttcaaggtca cctacaacca caagacctac accatggtgc cggaagagac gacgacgggc
      241 gccgtgcgcc tggtcgacct caccggcgcg gtcgtctgga tccagatccc gatgaagtcg
      301 atgctgatga accagaagga acaccaccgc ctggtcgacg gctgcgagga ggccgaacag
      361 cgcagcgccg tcgcggcgca gcagcagtcc accaacgccg ccgccaccgc tgccgcggcg
      421 cagacggccg cgccgggctc gaccatcacc accacgacca cgacgaccac ccaggtcgtg
      481 gcgccggcgc cggtgaagaa cgccgctgct gcaccgacga ccgccgcgtc gggcatgtgg
      541 aagtgactcc cacctgaggt gtccgagcca aggtgtctgg tgttgcatct cgcacggcat
      601 ggtgccggcg accgcaactg cgcacgtcgt gcgagatgcg agacctgaca ccgcgttgat
      661 cgcaagagaa gaaaacgccg cgcggcgcct gccgggcggc gtttttcttt gatcagtcgg
      721 tatcaggtct cgcatccggc gcggcgaggg accatcgcca gcgaagagcg cggcgcgccg
      781 gacgcaagac ctgacaccga agacgaagga ttcctgacgc cgaagacgaa ggattcctga
      841 caccgaagac gaaggattcc tgccaccgaa gacgaaggat tcctgacacc gaagacgtcg
      901 gactaactgt tcgacgtcgc ccgcacttcc tcgatcgtcg tcacaccggc cagcaccttc
      961 tcgatgccgt cctggcgcag cgtgcgcatg ccttcgccca tcgcgtgcat ctggatctgc
     1021 tcggcgcgcg cgccctgctg gatcaggtgg cgcacgccct tgctgacggt cagcagctcg
     1081 tgcacgccgg cgcggccctt gacgcccgtg ttgccgcact tggcgcagcc cgtgccgcgg
     1141 tagaactgca ggcggccgtc gacggcgaag cggctcgtcc agccagccag aaggtcgtcg
     1201 cggctcgggg cctgcgggtg gtcgccccac acgtgcagcg tgtcgttgag cagttcctcg
     1261 acctgttcgt cggtggccgg cgtggcggtc ttgcagccgc tgcagatgcg gcgcacgagg
     1321 cgctgcgcca gcaccgcgag cagcgcgtcg gcgaagttga acggatccat gcccatgtcg
     1381 agcaggcggg tgatggtctc gggtgcgctg ttggtgtgca gcgtggacag caccaggtgg
     1441 cccgtcagcg acgattcgac cgcgatctgc gcagtctcgt tgtcgcggat ctcgcccacc
     1501 atgatcacgt caggatcggc gcgcaggaac gcgcgcagcg ccttggcgaa ggtccagtcg
     1561 atcttcgggt tgacctgcac ctggcgcagg ccggcctgcg tgatttccac cgggtcctcg
     1621 gccgtccaga tcttgcgctc gggcgtgttg atgaagttca gcgccgagtg aagcgtggtc
     1681 gtcttgcccg agccggtcgg gcccacgcac agcaccatgc cgtacgggcg ctcgatggcg
     1741 cgcttcaggt gcgtcaggtt gtgcggcgac atgcccagcg aatcgagcgg cagcggcttg
     1801 gccgaggcga ggatgcgcag caccgcgtcc tccaggccgt tggccgtggg gatcatcgcg
     1861 acgcgcagct cgatcgggca ctgcggcacg aagcgcgcga agttgatctt gccgtcctgc
     1921 gggcggcggc gctcggagat gtccaggtcg cacatgatct tcaggcgcgc gaccagcgcc
     1981 gagcggtacg tgtgcggcag ctccaggtag ggccgcagca ggccgtcgac gcggaagcgc
     2041 accttgacct tctcgcggcc cgggcgcgtc tcgatgtgga tgtcggacac gccctggttg
     2101 tgcgcctcga cgatcatcga gttgatcagc cgcaccagcg agctgtcgga ctgctccacc
     2161 gcgccttcgt tctcgtcg
//
LOCUS       DATCRO010000698         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_356040, whole genome shotgun sequence.
ACCESSION   DATCRO010000698 DATCRO010000000
VERSION     DATCRO010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356040"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VIN75_18910"
     CDS             <1..298
                     /locus_tag="VIN75_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8976293.1"
                     /translation="VAAGLGGISGVLIGLYSNALFPLMGQPMLHKGIAVIILGGMGDI
                     RGAMFAGLFLGFAEVLSVAYIGSDMRDAVAFGLLFLVLLVRPKGLFGSVQERKV"
     gene            301..1173
                     /locus_tag="VIN75_18915"
     CDS             301..1173
                     /locus_tag="VIN75_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8976294.1"
                     /translation="MLDALDNFWSIYSNLVLSLGTASLLALSIYLTLSCGMLAMANAA
                     FMGIGAYTSSILTMNHGWPFPAAIAAGMAAPATVAFIIGKPTLRLSGVYLAMATLAFG
                     EVVRLIIIRAEDTTGGALGLNGIPQLTQWWHVLLAVVAVLFVLARLRRSKIGRAFEAI
                     KEDETAAGLMGIDVNGHKMLAFALGAAIAGLAGTLNAHLTFFIGPQEYGFDRGVEILT
                     MTILGGINSLFGPVLGGIIVTLLPELLRSFKDYRAVANGLILVLIVLFLPKGLWNPAW
                     LRRVLGLKRKGVSP"
     gene            1170..1973
                     /locus_tag="VIN75_18920"
     CDS             1170..1973
                     /locus_tag="VIN75_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8976295.1"
                     /translation="MTELLKLTNVSRHFGGLKVLQDVSFTVRQGAISGLIGPNGAGKT
                     TVFNLVTGLLPPTTGGIALDGATLLGRKPHEITRAGVARTFQNIRIFKEMTLLENVIV
                     GMHRHLAYGIPGLMFALPQYREQERRARERARELLSWMKLDHKADDLADNLSYGAQRR
                     LEIARALATEPRLLLLDEPVAGMNSTEKTELMREIVNIQGRGYTIFMIEHDMRFVMGL
                     CEEISVLNFGKIIAHGNPDQIRNDPQVIEAYLGREDDEVSVTPEEELDE"
     gene            1970..2689
                     /locus_tag="VIN75_18925"
     CDS             1970..2689
                     /locus_tag="VIN75_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8976296.1"
                     /translation="MSEALLSVTGLSVNYGHIEAVRELDLSLAAGQISTLVGANGAGK
                     STTLLALSGLIPKAAGKVVFDGHDVTALPAHRLVAAGMVQVAEGRATLTTLTVRENLE
                     LGAYTRRDGAAARASDLERVFTLFPRLKEREGGLAGNLSGGEQQMLAIGRALMARPRL
                     LLLDEPSMGLAPIVVQGIFRTLREIHAGGLTIFLVEQNVRQALKIAERAYVLENGRVV
                     LQGSGRELLDEPRVQQAYLGG"
     gene            complement(2674..>4585)
                     /locus_tag="VIN75_18930"
     CDS             complement(2674..>4585)
                     /locus_tag="VIN75_18930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976297.1"
                     /translation="RGREAEVHARLADMVAGLPSVVNLNVWDARGRPLARSDRFPTDR
                     VVSVEDRPYFMRQKTAPVPLGVSEVLAGRQTGHELVNATIRRTGVDGQFGGVVAVSLA
                     PAFFRDYYRSLAADNPALARLALLRTDGAILASWPSVRAGEDRRVAVDDAVFTHVRAG
                     ERSGQIEVRAADDGDDTRIVSFQRVGDFPLYVAVGVSRAAMLAGWLRFVGLLAAVLVP
                     VTAGLTYVSIVALRKTRSEQAITAALNDAVARRAAAEKGMLESQKLETLAQLTGGVAH
                     DFNNLLAIVSSSLHVHRHLHPEQADERHLQAVSRAVQSGTRLTRQLLSFSRKQALRPE
                     LITLQTWLPGAEGLLRSTLGSRIAMEIVVEPEVCAIRVDAAELELALINLALNAKHAM
                     PGGGRLRISARNEGASEDGTSMVVIGVADTGVGIAAEVLPRVFEPFFTTRMHEVGSGL
                     GLSQVHGFCAQAGGHARIASVLEQGTTVDMVLPAEAPQPHPPTLAQDTDAGPLSGRVM
                     LVEDNEDVAHSTAAILQASGLDVAHHWSADAALAALHEGSALPDIVLSDIEMPGRLSG
                     MDLAFRLRERWPRLPVVLVTGYAKQLEEAVSGGLRVMPKPTPPLDLLRELRRAMTEAA
                     RRTPAAQAQPPR"
BASE COUNT          653 a         1662 c         1602 g          668 t
ORIGIN      
        1 cgtcgccgcc ggcctgggcg gcatctcggg cgtgctgatc gggctgtact cgaacgcgct
       61 gttcccgctg atgggccagc cgatgctgca caagggcatc gccgtcatca tcctgggcgg
      121 catgggcgac atccgcggcg ccatgttcgc cggcctgttc ctcggcttcg ccgaggtgct
      181 gtcggtcgcc tacatcgggt cggacatgcg tgatgcggtg gcattcggcc tcctgttcct
      241 cgtgctgctc gtgcgcccca agggcctttt cggcagcgtg caggaacgca aggtctgacg
      301 atgctcgacg ctctcgacaa cttctggtcc atctacagca acctcgtgct gtcgctgggc
      361 acggcgtcgc tgctggcgct gtcgatctac ctcacgctgt cgtgcggcat gctggcgatg
      421 gccaacgcgg cgttcatggg gatcggcgcg tacacctcgt ccatcctcac gatgaaccac
      481 ggctggccgt tcccggcggc catcgcggcg ggcatggcgg cgccggccac cgtggccttc
      541 atcatcggca agcccacgct gcgcctgtcg ggggtctacc tggcgatggc cacgctggcc
      601 ttcggcgagg tcgtgcggct gatcatcatc cgcgccgagg acaccaccgg cggcgcgctg
      661 ggcctgaacg gcatcccgca gctgacccag tggtggcacg tgctgctggc ggtggtcgcg
      721 gtgctgttcg tgctcgcccg gctgcggcgc tcgaagatcg gccgcgcctt cgaggccatc
      781 aaggaggacg agaccgccgc cggcctgatg ggcatcgatg tcaacggcca caagatgctc
      841 gcgttcgcgc tcggcgcggc catcgcgggc ctggccggca cgctcaacgc ccacctgacc
      901 ttcttcatcg gcccgcagga atacggtttc gaccgcggcg tcgagatcct gaccatgacg
      961 atcctgggcg gcatcaacag cctgttcggc ccggtgctgg gcggcatcat cgtgacgctg
     1021 ctgcccgagc tgctgcgcag cttcaaggac tatcgcgcgg tcgccaacgg cctgatcctc
     1081 gtgctgatcg tgctgttcct gcccaagggg ctgtggaacc cggcgtggct gcgccgcgtg
     1141 ctggggctca agcgcaaggg agtcagccca tgacggagct cctgaagctg accaacgtct
     1201 cgcgccattt cggcggcctc aaggtgctgc aggacgtgtc gttcaccgtg cgccagggcg
     1261 ccatctccgg cctcatcggc ccgaacggcg ccggcaagac caccgtgttc aacctcgtca
     1321 ccggcctgct gccgcccacc accggcggca tcgcgctgga cggcgccacg ctgctgggcc
     1381 gcaagccgca cgagatcacg cgcgccggcg tggcgcgcac gttccagaac atccgcatct
     1441 tcaaggagat gacgctgctg gagaacgtca tcgtcggcat gcaccgccac ctcgcctacg
     1501 gcattccggg cctgatgttc gcgctgccgc agtaccgcga gcaggagcgc cgcgcccgcg
     1561 agcgcgcccg cgaactgctg tcgtggatga agctcgacca caaggccgac gacctcgccg
     1621 acaacctgtc gtacggcgcc cagcgccggc tcgagatcgc ccgcgccctg gccaccgagc
     1681 cgcgcctgct gctgctggac gagccggtgg ccggcatgaa cagcaccgag aagacggagc
     1741 tgatgcgcga gatcgtcaac atccagggcc gcggctacac gatcttcatg atcgagcacg
     1801 acatgcgctt cgtcatgggc ctgtgcgagg agatctccgt gctgaacttc ggcaagatca
     1861 tcgcccacgg caacccggac cagatccgca acgatccgca ggtgatcgag gcctacctgg
     1921 gacgcgagga cgacgaggtg tcggtcacgc ccgaagagga gctcgacgaa tgagcgaggc
     1981 gctcctctcc gtcaccggcc tgagcgtcaa ctacggccac atcgaggccg tgcgcgagct
     2041 ggacctgtcg ctggccgcgg gccagatctc gacgctggtc ggcgccaacg gcgcgggcaa
     2101 gtcgacgacc ctgctggcgc tgtcgggcct gatccccaag gccgcgggca aggtcgtgtt
     2161 cgacggccac gacgtcaccg ccctgcccgc ccaccggctg gtggccgccg gcatggtgca
     2221 ggtcgccgag ggccgcgcca cgctgaccac gctgaccgtg cgcgagaacc tcgaactcgg
     2281 cgcctacacg cggcgcgacg gcgcggccgc gcgcgcgagc gacctcgaac gcgtgttcac
     2341 gctgttcccg cggctcaagg agcgcgaagg cggcctcgcc ggcaacctgt ccggcggcga
     2401 gcagcagatg ctggcgatcg gccgcgccct gatggcccgg ccgcgcctgc tgctgctgga
     2461 cgaaccctcg atgggcctgg cgcccatcgt cgtgcagggc atcttccgca cgctgcgcga
     2521 gatccacgcc ggcggcctga cgatcttcct ggtcgaacag aacgtgcgcc aggcgctcaa
     2581 gatcgccgag cgcgcctacg tgctggagaa cggccgcgtc gtgctgcaag gcagcggccg
     2641 cgagctgctc gacgagccgc gcgtgcagca agcctacctg ggcggctgag cctgggcggc
     2701 cggcgtccgt cgcgcggcct cggtcatcgc ccggcgcaat tcgcgcagca ggtccagcgg
     2761 cggcgtcggc ttgggcatca cgcgcagccc gcccgacacc gcctcctcga gctgcttggc
     2821 gtagccggtc acgagcacca cgggcagccg cggccagcgt tcgcgcagcc ggaacgcgag
     2881 gtccatgccg ctgagccggc ccggcatctc gatgtcgctg agcacgatgt cgggcagcgc
     2941 cgagccttcg tgcaaggccg ccagcgccgc gtcggcgctc cagtggtgcg cgacgtccag
     3001 ccccgatgcc tgcaggatgg cggcggtcga atgggcgacg tcctcgttgt cctcgaccag
     3061 catcacgcgc cccgacagcg ggcccgcgtc ggtgtcctgc gccagcgtcg gcggatgggg
     3121 ctgcggcgcc tcggccggca gcaccatgtc gaccgtcgtg ccctgctcga gcacgctggc
     3181 gatgcgcgca tggccgccgg cctgggcgca gaacccgtgc acctggctca ggcccaggcc
     3241 cgagccgacc tcgtgcatgc gcgtggtgaa gaacggctcg aacacgcggg gcagcacctc
     3301 ggcggcgatg cccacgcccg tgtcggcgac gccgatcacc accatcgacg tgccgtcctc
     3361 cgacgcgcct tcattgcgcg cggagatgcg caggcggccg ccgccgggca tcgcgtgctt
     3421 ggcgttcagc gcgaggttga tcagcgccag ctcgagttcc gccgcgtcga cgcggatcgc
     3481 gcacacctcg ggctcgacga cgatctccat ggcgatgcgc gagcccagcg tgctgcgcag
     3541 cagcccctcg gcgccgggca gccacgtctg cagcgtgatg agctccggcc gcagcgcctg
     3601 cttgcgcgag aacgacagca gctggcgcgt caggcgcgtg cccgactgca ccgcgcgcga
     3661 caccgcctgg aggtggcgct cgtcggcctg ctccggatgc aggtggcgat gcacgtgcag
     3721 gctgctgctg acgatggcca gcaggttgtt gaagtcgtgc gcgacgccgc cggtcagctg
     3781 cgccagcgtc tcgagcttct ggctctccag catgcccttc tcggccgcgg cgcgacgcgc
     3841 gacggcgtcg ttgagcgccg ccgtgatggc ctgctcgctg cgggtcttgc gcagcgcgac
     3901 gatcgacacg taggtcaggc cggcggtcac cggcaccagc accgctgcca ggagcccgac
     3961 gaagcgcagc cagccggcca gcatcgcggc gcggctgacg cccacggcga cgtagagcgg
     4021 gaagtcgccc acgcgctgga agctgacgat gcgcgtgtcg tcgccgtcgt cggccgcgcg
     4081 cacctcgatc tggccgctgc gctcgcccgc ccggacgtgc gtgaagaccg cgtcgtcgac
     4141 cgccacgcgg cgatcctcgc ccgcgcgcac cgagggccag ctggcgagga tcgcgccgtc
     4201 ggtgcgcaga agggccaggc gcgcgagcgc cgggttgtcg gcggccagcg agcggtagta
     4261 gtctcggaag aaggccggcg ccagggagac cgccacgacg ccgccgaact ggccgtcgac
     4321 gcccgtgcgc cggatggtgg cgttgacgag ctcgtgcccc gtctggcggc cggcgagcac
     4381 ctcgctgacg cccagcggca ccggggccgt cttctggcgc atgaagtacg ggcggtcctc
     4441 caccgagacg acgcggtcgg tcggaaaccg gtcgctgcgc gccagcggac ggccccgggc
     4501 gtcccacacg ttcaggttca cgaccgacgg caggccggcg accatgtcgg ccaggcgggc
     4561 atggacctcg gcctcgcggc cgcgc
//
LOCUS       DATCRO010000699        11869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_806323, whole genome shotgun sequence.
ACCESSION   DATCRO010000699 DATCRO010000000
VERSION     DATCRO010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806323"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VIN75_18935"
     CDS             <1..360
                     /locus_tag="VIN75_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976298.1"
                     /translation="AGGVTGSGVNELAIFAEDGTLIDQTGDMTAAWSAAGMVDGALSG
                     SHTVTAGTRYYLGFLTHFSGSSPHCAATGTAQTANFPVANTHRTAIFKSGVSAIPTSF
                     DPTTYTPNSGYFILYAR"
     gene            375..896
                     /locus_tag="VIN75_18940"
     CDS             375..896
                     /locus_tag="VIN75_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976299.1"
                     /translation="MANPDHGNDGRFTRTIEGAERDGEAARLKSRGLTYAQIARNLDY
                     ADASGAYRAVQRALAAAPVEGGDELRRLQSDLIDALTAQAFEVLESTHYAHTVHGDLI
                     HGPDGQPMIDDMPVLHAIDRLIRLAERRSKLMGLDAPSRHEVITLDYLDAQIRDAAAE
                     LARAEAGEASDSA"
     gene            856..2502
                     /gene="terL"
                     /locus_tag="VIN75_18945"
     CDS             856..2502
                     /gene="terL"
                     /locus_tag="VIN75_18945"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEY8976300.1"
                     /translation="MLELRLEKLQTLRELRKRQLELRRATARKQYATPGLLAAAIERG
                     TVQTPALDLIDAALVDVAEGRCDRLIISMPPQEGKSTRVTTTTPLWLLLRNPDLRIAI
                     ASYGQDLAEEFGQAIRDFITGNSGEDGTLDLGLRIAPDNGAARRWKLDGHRGGVRSVG
                     LSSGLTGRPVDVLFIDDPIKGQDDADSEVWRERVWKWWQTVGATRLAPGAPVVLVLTR
                     WHEDDLAGRLLAAEDASRWRVINIPALADHDPAKGQADPLGRKPGEWLQSARGRTEQQ
                     WREIRVQAGSRAFTALYQGHPSPDKGNVWLRQWWRRYSVPLWSQHPDIPGAWIVAECD
                     EVLISADMAFKDTKGSDYVAIGVWVRRGAQVFLVDQIRKRLSFTETLTAFSGLCAKWP
                     QAAAKLVEDKANGTAVIDSLRKKIPGIIAVNPKESKYARAEAVAPFVEAGNVFLPDGE
                     IALFDVDAFIDEAAGFPRAAHDDQVDQTSQALGRMLLRVGPGAAYLEAMKRRAAQSGL
                     TVPPQTRSWRTARQSTRGEGAENGEAPQVSPGRPLPPPRR"
     gene            2447..5746
                     /locus_tag="VIN75_18950"
     CDS             2447..5746
                     /locus_tag="VIN75_18950"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY8976301.1"
                     /translation="MAKRHKSRRGAPSRPRAASQPQTGRALRKADLATAPRTPAIIED
                     SLSRAGMHNSAQMGPGRPLNPYQGYSQAPRRTDYPVSVNISTQTRASWDRISYDTLRA
                     IVKAYDVARICINHKIDELRSMELNFSAADWARGDVDTALEAARAALAFPDRELPYED
                     WLSKWLENALKFDAAPLYRRRNMDGEVIGLEVLDGTTVMPYIDENGRKPEPPGPAYYQ
                     VIHGMPWNWYTRDDIDFRMFRPQEDSPFGLAPMESILLTANTDLRFQWHFLQMFTEGS
                     VPAGFLELPPDISSPDQVAEWQDYWNAVVLGDQAKLNELIAVPNGTKLQETRPRAFDK
                     AFPQYLMQRTCAAYGIVPQDVGLIEDVNRSNGETQTDIQFRVNTLPWVYWVQATLTRY
                     LQYDLHLPVKVELNTGRDVEDRVAEAQAWQIYIESGMASPDEGREKVLGLPIDKSRPT
                     PRFFSTTRSGPIPLLAIEGLSGRTDADTYGPADDQPVIRAPFIAPPGVIPAQGTSDAQ
                     QSVAAEDAYQTSMRQQLQAEGPQPDATPQTQDSAVVQQVAKSERDRELAAFRQFAAER
                     RRRGRWRDFEFRSEQVDLLTARRLNNAGRLAVCKSLGAVAVAGFAVRAADTGRVLMLQ
                     RALNPRTCPCGMPLAWGMDGGDWWSHADGSISHDVPFAGSSVSDLVGTEPPDPAAGTW
                     EFPGGHIEGGEYPLEAARREWAEETGCLPPLGEQTGVWYSPNGIYQGIVWTVPSEADV
                     PITTGRGVVSNPDDPDGDQIEALAWWDPEQLPGNPALRPELLESIDRALAALATGPDG
                     VAKADPDPKDQAPPQGPDSAQQWPGWQYDLAAAAYWAPLIAAALGDSIDPRTVAAEFA
                     ARVPVSVAPEGESAEQREQRLREVAAEFVEQYRAAIQRAVRSVLIGTYVDGYAIGSAS
                     ATAVMDAAEAGLPAADVAADAAGWAPGNYEAAMLLIGRDADGSGLRDLLDKAQIEIKS
                     IADTRLNELGRLLAVGAARGDSADTIARDIRDLLTAPQRAQMIATTELARAVSTAAVD
                     GYQRRGYRETEWLTAEDERVCPEVCEPNEEAGPVRIGHPFPSGAFQPPGHPWCRCAPG
                     VVISSRRQEGE"
     gene            5747..6124
                     /locus_tag="VIN75_18955"
     CDS             5747..6124
                     /locus_tag="VIN75_18955"
                     /inference="COORDINATES: protein motif:HMM:NF025901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HEY8976302.1"
                     /translation="MADEQRYVLGIAYQAGPDPRIRKGMDGARDFISADELEKAAWSY
                     LRTGGQVGIEHVDGTTGHATVVESYIYRGPDWDVGDGVVVKAGDWVLGAVLDETAWSL
                     YKQGKVTGWSPQGHARRIPPRSS"
     gene            6124..7818
                     /locus_tag="VIN75_18960"
     CDS             6124..7818
                     /locus_tag="VIN75_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976303.1"
                     /translation="MADTVIDDDPFSELVDVTVPQVDMVGAAANGTPWFFAKSEGGAS
                     LFPPDFVRELISTSESSGEAVTMTGTPAALADTMARMHEAAVRKAKYNTDDRKRMSGN
                     GQAMDDGSYPIADEEDLDNAIHAVGRGSGGHDAIRRHIISRAKSLGASSKIPDNWGSD
                     GSLKTGVSKADGEPDDAGALDTATVLAEPEGVGEGNPETPGSPAWEAIDAATARKWTA
                     IAVRLRQALDVMANREMVEGAAADPGDFLAASDLEDARCAVDFVIDTLAGFAVDEQAE
                     ADLGGTLDVIGKALSEFEPDHLEIVEGFAPLLKAGRVLSAANEKALRDAATAIQQVLA
                     RLPAPESDDGEPVAKRQETAVSDTATIEAPESAPAETPTAPEPAEAVTKADGEMVAVY
                     DSSGCLVGVVDKDAITNVVQPQAPDGGGMDEVEDAAGDNAAMIPGTHTVASPPLDDDD
                     EMTKAAQPSELATLTEAVNALAKQVAGKDDLADVVKGLQERVEHLAKMPDDRKSPLLN
                     GATGTAGVADRDGSTEDPYADLRKSIEQAKTPAAKREAEQQLAYARVRDRFTRPGA"
     gene            7913..9370
                     /locus_tag="VIN75_18965"
     CDS             7913..9370
                     /locus_tag="VIN75_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976304.1"
                     /translation="MSVGDDITAETIEQIKKAQTAGVTSGTGILGVDLSDLISLIPVN
                     TPFRDQLARTKPKMGAKFAEWRALVNINNTQPNPFTAFDYAAPIANVDEMDVTALYAK
                     IGMGYTVTQDAIDLAGGYADAKAVAVFNALNQFKIGEDKGGIGGCLFALTTPATPTVS
                     QSDTGGSIAASTAVNVKVAARTGANYFWGGSTVASAQGTVTTSTVAAATHSATATVAA
                     VRGAVAYDWFVAGFYYTTTTVNTVTITSIPVANQAVPNLPDIYGTAPAAVPVADSSAG
                     ANQFNGLLATLAGDYATGGATGLVTPGSGVTSGAYFHSLDGAAFTVSGASVKELDDLN
                     QSIYDTVRLSPDAYMMSSQQGTELSNVILSTTSSETFFQPNLAGRSEAVLGAFVGWYV
                     NKAAGGTPIRVEVHPHMPPGTLIARTDRVPFPNSNITNTLEMRCLRDLADYEYAANRQ
                     ANTAGGGPRFDGETYANETLINKAPVAMGVLQNIK"
     gene            9440..9817
                     /locus_tag="VIN75_18970"
     CDS             9440..9817
                     /locus_tag="VIN75_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976305.1"
                     /translation="MRIASTNGATALQHDGVDYEADENGVFDVPHEVGQVLVRFPHWL
                     PEQESAANRRALAAQAELDPAGLAARVRELEGQLASRQPSEGESEHLARIAELEAEVE
                     SAKAAKPAAKKTAAAKKADDAGE"
     gene            9814..10290
                     /locus_tag="VIN75_18975"
     CDS             9814..10290
                     /locus_tag="VIN75_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976306.1"
                     /translation="MSVIVPYTRLHPAAAILLDRHAPGHVRVPLDPADMSAYWRLLAE
                     QWHEPGDLLIVEHDVGIHADVVEGLTACPEPWCGHPYRIGSQLLVCLGCTRFSAALKA
                     SEPDLLDVVGEDGTGGLPARDWRRLDVRLLDELHRRGYRQHEHQPAVTHFHKYPGA"
     gene            10297..11190
                     /locus_tag="VIN75_18980"
     CDS             10297..11190
                     /locus_tag="VIN75_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976307.1"
                     /translation="MSVQPAVAPYVPTYATETSYITVDEYLAEPTGVDTSQLVPEGSA
                     DDNRAALARVIARASSQADSICRKPLAATVDVQSGEYRVRADGTIWVPVDYAPIVQVN
                     AISLGWSQGAMAAMTDLSGVRVERKLVKIPVAGAGASWNGSPRVSSRRGFIFADVTYV
                     NGWAVTATSQASLAGASSLSLAGSLGIAAGLRLSIADGANTESTTVGASYVPGSLTVP
                     TAAPLLYAHGAGVSVSALPGAGKDAVILLTSSRIKLRGAEAVQMGSISAEPSAPQPTM
                     PGGTSEYNQAVELLKPLRRAR"
     gene            11187..11732
                     /locus_tag="VIN75_18985"
     CDS             11187..11732
                     /locus_tag="VIN75_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976308.1"
                     /translation="MSRATVRAALYAYLQAGCAGGGSVPGVTKVYRAQPWLIDGKTWN
                     LSSDLGSGAVVFLHFMDKGETRQADPAASVSTEVVGWKQVDYQVALVVLYQYLIPSNT
                     QSVQYTGEEWVDALDATLGGLEDLLRADPTAGTGPGGAVFEMAQSPGDLTLPQDLPRL
                     VNGKVMSWQALHFTATEMIQA"
BASE COUNT         1954 a         4267 c         4113 g         1535 t
ORIGIN      
        1 gccggcgggg tcaccggttc cggggtgaac gagctcgcga ttttcgccga ggacggcacg
       61 ctgatcgatc agaccggcga catgacggcc gcgtggtctg cggcgggaat ggtcgacggg
      121 gcgctgagcg gctcgcacac cgtgacggcg ggaacccgct actacctggg attcttgacc
      181 cacttcagcg ggagttcgcc gcactgcgcg gcgacgggta ccgcccagac cgcgaacttt
      241 ccggtggcca acacgcaccg aaccgcgatc ttcaagagcg gggtttcggc cattccgacg
      301 agcttcgacc cgacgacgta cacgcccaac tccgggtact tcatcctgta cgcgagatag
      361 cggaggtcgc agccatggca aatcccgacc atggcaacga cgggcgcttc acgcggacta
      421 tcgagggcgc cgagcgggac ggcgaagcgg cgcgcctgaa gtcgcgaggt ctgacctatg
      481 cgcagattgc acgcaacctt gattacgccg acgcgagtgg tgcataccga gccgtccagc
      541 gggcacttgc cgcagccccg gtcgagggcg gcgacgagtt acgtcggctc cagtcggact
      601 tgatcgacgc actgaccgcg caggcgttcg aggtgcttga gtcgacgcac tacgcccaca
      661 ccgtgcatgg cgacctcatc cacggccccg acggccagcc aatgatcgac gatatgccgg
      721 tgctgcacgc gatcgaccgg ctcatccgcc tcgctgagcg ccgctccaag ctcatgggcc
      781 tggacgcccc gtcgcggcat gaggtgatca cgcttgacta cctcgacgct cagatccgag
      841 acgccgccgc ggagcttgct agagctgagg ctggagaagc ttcagactct gcgtgagctg
      901 cggaagcggc aactggaact gcggcgggcg acggcacgca agcagtacgc cacgccaggc
      961 ctattggccg ccgcgatcga gcgcggcacc gtacagaccc cggcgctgga cctgatcgac
     1021 gcggccctgg tggacgtcgc tgaggggcgc tgcgaccggc tgatcatcag catgccgccg
     1081 caggagggca agtccacccg ggtcacgacg acaacgccgc tgtggctgct gctgcgcaac
     1141 ccggacctgc gcatcgccat cgcgtcctac ggccaggacc tcgccgaaga gttcgggcag
     1201 gcgatccgcg acttcatcac cggcaacagc ggcgaggacg gcacgctcga cctcggtctg
     1261 cgtatcgccc cggacaacgg cgcggcgcgg cggtggaagc tcgacgggca ccggggcggc
     1321 gtacgctcgg tgggtctcag ctccggcctg accgggcgtc cggtggacgt gctgttcatc
     1381 gacgacccga tcaaagggca ggatgacgcc gactccgagg tctggcgcga gcgcgtctgg
     1441 aagtggtggc agaccgtcgg cgccacacgt ctggcccccg gtgcgcccgt agtgctggtg
     1501 ctcacccgct ggcacgagga cgacctcgcc ggacgcctgc tcgccgcgga ggacgcctcc
     1561 cgctggcgcg tgatcaacat tccggcgctc gccgaccacg accccgccaa gggccaggcc
     1621 gatccgctcg gccgcaagcc cggtgaatgg ctgcaatccg cgcgcggacg taccgaacag
     1681 cagtggcgcg aaatccgcgt gcaggccgga tcccgggcgt tcactgcgct ctaccagggc
     1741 catccgtcgc cggacaaggg caacgtgtgg ctgcgtcagt ggtggcgccg ctacagcgtg
     1801 ccgctgtggt cgcagcaccc cgacatcccc ggcgcgtgga tcgtcgcgga gtgtgacgag
     1861 gttctgatca gtgccgacat ggcgttcaag gacaccaagg gcagcgacta cgtcgccatc
     1921 ggcgtgtggg tgcgccgcgg cgcgcaggtg ttcctggtcg accagatccg caagcgcctc
     1981 tcgttcaccg agaccctgac ggcgttctcg ggcctgtgcg cgaagtggcc gcaggcggcg
     2041 gcgaagttgg tcgaggacaa ggccaatggc actgcggtca tcgacagcct tcgcaagaag
     2101 atccccggga tcatcgcggt gaacccgaag gagtcgaagt acgcacgcgc cgaggcggtc
     2161 gcgccgttcg tcgaggcggg caacgtgttc ctgcccgatg gcgagatcgc gctgttcgac
     2221 gtggacgcct tcatcgacga agccgcaggg ttcccgcgcg ccgcccacga cgaccaggtc
     2281 gaccagacca gccaagcgct cgggcggatg ctgctccggg tcggccccgg agccgcgtac
     2341 ctcgaagcca tgaagcgcag ggccgcgcag tccggcctca ccgtcccgcc ccagacccgc
     2401 agttggcgca ccgcccggca gtccacacga ggggagggcg ccgagaatgg cgaagcgcca
     2461 caagtctcgc cggggcgccc cctcccgccc ccgcgccgct agccagccgc agaccggccg
     2521 ggcgctgcgc aaagccgacc tcgccaccgc gccgcgtacc cccgccatca tcgaggactc
     2581 gctctcgcga gccgggatgc acaactcggc gcagatgggg cccggccggc cgctgaaccc
     2641 ctaccagggg tattcgcagg cgccgcggcg caccgactac ccggtcagcg tcaacatctc
     2701 cacgcaaacc cgcgcgtcgt gggatcggat cagctacgac acgctgcgcg cgatcgtcaa
     2761 ggcgtacgac gtcgcccgga tctgcatcaa ccacaagatc gacgagctca gaagcatgga
     2821 gctgaacttc tccgccgccg actgggcgcg cggagacgtc gacaccgccc tcgaagccgc
     2881 ccgcgccgca ctcgcgttcc ccgaccggga gttgccctac gaggactggc tgagcaagtg
     2941 gctggagaac gcgctcaagt tcgacgccgc cccgctctac cgacgccgca acatggacgg
     3001 cgaggtcatc ggcctggaag tgctcgacgg cacgacggtg atgccgtaca tcgacgagaa
     3061 cggccgcaag cccgagccgc ccggtcccgc gtactaccag gtcatccatg ggatgccgtg
     3121 gaactggtac acgcgcgacg acatcgactt ccgcatgttc cgcccgcagg aggactcccc
     3181 attcggcctc gcgccgatgg agagcattct gctgacggcg aacacggacc tgcggttcca
     3241 gtggcacttt ctgcagatgt tcaccgaggg gtccgtgcct gctggcttcc ttgagctgcc
     3301 gccggacatc tccagccccg accaggtggc ggagtggcag gactactgga acgcggtcgt
     3361 gctcggcgac caggcgaagc tcaacgagct gatcgcggtg ccgaacggca cgaagcttca
     3421 ggagacccgt ccgcgggcct tcgacaaggc attcccccag tacctgatgc aacgcacctg
     3481 cgccgcgtac ggcatcgtgc cgcaggacgt gggcctgatc gaggatgtca accggtccaa
     3541 cggcgaaact cagaccgaca tccagttcag ggtcaacacc ctgccgtggg tgtactgggt
     3601 gcaggcgacg ctgacccggt atttgcagta cgacctgcac ctgccggtca aggtcgagct
     3661 gaacaccggc cgcgacgtgg aggaccgtgt cgccgaggcg caggcctggc agatctacat
     3721 cgagtccggc atggcctccc ccgacgaggg ccgcgagaag gtgctcggcc tgccgatcga
     3781 caagtcacgg ccgacgccgc ggttcttctc caccacccgc tccggcccga tcccgctatt
     3841 ggcgatcgag ggcctgtccg gtcgcacgga cgcggacacc tacggcccgg ccgacgacca
     3901 gccggtgatc cgcgccccgt tcatcgcacc gcccggcgtg atccccgcgc agggcaccag
     3961 cgacgcgcag cagtcggtgg cggccgagga cgcctatcag acgtcgatgc gccagcagtt
     4021 gcaggccgag gggccgcagc ccgacgccac gccgcagacg caggactcgg cggtagttca
     4081 gcaggtggcc aagagcgaac gggaccggga gcttgccgcg ttccgccagt tcgccgccga
     4141 gcgcagacgc cgtgggcgct ggcgcgactt cgagttccga tccgagcagg tggatctgct
     4201 caccgcgcgc cggctgaaca acgcgggcag gctcgcggtc tgcaagtcgc tcggcgcagt
     4261 ggcggttgcg ggcttcgcgg tgcgcgcagc cgacacgggc cgtgtgctga tgctgcagcg
     4321 ggcgctgaac ccgcgcactt gcccatgcgg aatgccgctg gcgtggggca tggacggcgg
     4381 cgactggtgg tcgcatgccg acggctcgat cagccacgac gtgccgttcg cgggctccag
     4441 cgtctccgac ctggtcggca ccgagccgcc cgaccctgcc gccgggacgt gggagttccc
     4501 cggcggccac atcgaaggcg gcgaataccc gctggaggcg gcgcggcgcg agtgggccga
     4561 agagaccgga tgcctgccac cgctcggcga gcagaccggg gtctggtaca gcccgaacgg
     4621 gatctaccag ggcatcgtct ggacggttcc gtccgaggcc gacgtgccga tcactaccgg
     4681 gcgcggcgtg gtcagcaacc cggacgaccc ggacggcgac cagatcgagg cgctggcgtg
     4741 gtgggatccg gagcagttgc cgggcaaccc ggcgctgcgc ccggagctgc tggagtcgat
     4801 cgaccgggca ctggcggcgc tcgccacggg accggacggt gtcgcgaagg cggaccccga
     4861 cccaaaagac caggccccgc cgcaggggcc tgacagtgca cagcagtggc cgggctggca
     4921 gtacgacttg gccgcagccg cctactgggc accgctgatc gcggcggcgc tcggcgactc
     4981 gatcgacccg cgcacggtcg cagcggagtt cgccgcacgg gttcccgtca gcgtcgcgcc
     5041 cgaaggcgaa agcgccgaac agcgcgaaca gcggctgcgc gaggtggcgg ccgagttcgt
     5101 cgagcagtac cgcgccgcga tccagcgggc cgtgcggtcc gtcctgatcg gcacgtacgt
     5161 ggacggctac gcgatcggct cggccagcgc cacggccgtc atggacgccg ccgaagccgg
     5221 actgccggct gccgatgtag cggccgacgc ggcgggctgg gcgccgggca actacgaggc
     5281 ggcgatgctg ctgatcggcc gcgacgccga cggctccggg ctgcgcgacc tactcgacaa
     5341 ggcgcagatc gagatcaagt ccatcgcgga cacgcgcctg aacgaactcg ggcgcctgct
     5401 cgccgtcgga gcggcgcgcg gcgactcagc ggacacgatc gcgcgcgaca tccgcgacct
     5461 gctcaccgcg ccgcagcgcg cgcagatgat cgccaccacc gagctggcgc gcgccgtgtc
     5521 caccgcggcc gtggacggct accagcggcg cggctaccgc gagaccgagt ggctcaccgc
     5581 cgaggacgag cgggtgtgcc cggaggtctg cgagccgaac gaggaagcgg gcccggtgcg
     5641 catcggccac ccgttcccct ccggcgcgtt ccagccgccc ggccacccgt ggtgccgctg
     5701 cgcgcccggt gtcgtcatct cttcgcgcag gcaggagggc gagtagatgg cggacgaaca
     5761 gcgctacgtc ctcggcatcg cctaccaggc cggacccgat ccgcgcatcc gcaagggcat
     5821 ggacggcgcc cgcgacttca tcagcgccga cgagctggag aaggccgcgt ggagctacct
     5881 gcgcaccggc ggacaggtcg gcatcgagca cgtggacggc acgacgggcc acgccacggt
     5941 cgtcgagtcg tacatctacc gcggcccgga ctgggacgtc ggcgacggcg tggtggtcaa
     6001 ggcgggcgac tgggtgctgg gcgccgtgct cgatgagacc gcctggagcc tgtacaagca
     6061 gggaaaagtc accggctggt ctccgcaggg ccacgcccgc cgcatccctc cccggagtag
     6121 ctgatggccg acacggtgat cgacgacgac ccgttcagcg agctggtcga cgtcaccgtc
     6181 ccgcaggtgg acatggtcgg cgccgcggcg aatggcacgc cctggttttt cgccaagtcc
     6241 gagggtggcg cgtccttgtt cccgcccgac ttcgtgcgcg aactgatcag cacatcggag
     6301 tccagcgggg aggcggtgac gatgaccggc acccccgccg ctctcgccga cacgatggcg
     6361 cggatgcacg aggccgcggt gcgcaaggcc aagtacaaca ccgacgaccg caagcgcatg
     6421 tccggcaacg gccaggcgat ggacgacggt tcgtacccga tcgccgatga ggaagacctc
     6481 gataacgcga tccacgccgt cgggcgcggc tccggcggcc acgacgcgat ccggcggcac
     6541 atcatctccc gcgcgaagtc gctgggtgcc tcgtcgaaga tcccggacaa ctggggcagc
     6601 gacggcagcc tgaagaccgg cgtttcgaag gccgacggcg aaccggacga cgctggcgcg
     6661 ctcgacacgg ccacagtgct ggccgaaccc gaaggcgtcg gcgaaggcaa ccccgagacg
     6721 cccggctccc ccgcgtggga ggccatcgac gcggccaccg cgcgcaagtg gaccgcgatc
     6781 gccgtgcggc tgcgccaagc gctggacgtc atggcgaacc gggagatggt cgagggcgcg
     6841 gcggccgatc cgggcgactt cctcgccgcc agcgacctgg aagacgcgcg ctgcgccgtg
     6901 gatttcgtca tcgacaccct cgccgggttc gccgtggacg agcaggccga ggccgatctc
     6961 ggcggcacgc tcgacgtcat cggcaaggca ctgtccgagt tcgagccgga ccacttggag
     7021 atcgtcgagg gtttcgcgcc gctgctcaaa gccgggcgtg tgctgtcggc cgcgaacgag
     7081 aaggcgctgc gcgacgcggc gaccgcgatc cagcaggtgc tcgcgcgcct gccagccccc
     7141 gaatccgacg acggcgagcc cgtcgcgaag cgtcaggaga ccgccgtgtc cgacaccgcc
     7201 accatcgagg cccccgagag cgcgccggcc gagacgccca ccgcgcccga gccggccgaa
     7261 gccgtcacga aggccgacgg cgagatggtc gccgtctacg acagcagcgg ctgcctggtc
     7321 ggcgtcgtcg acaaggacgc catcaccaac gtcgtccagc cgcaggcccc cgacgggggc
     7381 ggcatggacg aagtcgagga cgcagccggc gacaacgccg ctatgatccc cggcacccac
     7441 accgtcgcct ccccgccgct ggacgacgac gacgagatga cgaaggcagc acagccgtcc
     7501 gagctggcca ctctcacaga ggccgtgaac gcgctcgcga agcaggtcgc cggcaaggac
     7561 gacctggcgg acgtggtcaa gggcctacag gagcgtgtcg agcacctggc gaagatgcct
     7621 gacgaccgca agtcgcccct gctcaacggc gcgaccggca ctgccggcgt cgccgaccgc
     7681 gacggatcca ccgaggaccc gtacgccgac cttcgcaagt cgatcgagca ggccaagacc
     7741 cctgcggcga agcgggaggc cgagcagcag ctcgcctacg cgcgcgtgcg cgaccgcttc
     7801 acccgccccg gcgcatagcc ggacgcgcgg caccttcccc gcacgcttcc ccgacagccc
     7861 gcccgccgta gcgcggaacg ggccggtcgg catgcccaga aaggggcacc ccatgagcgt
     7921 cggcgacgac atcaccgccg agaccatcga gcagatcaag aaggctcaga ccgcgggcgt
     7981 gacctccggc accgggatcc tcggtgtcga cctgtccgac ctgatctccc tgatcccggt
     8041 caacacccca ttccgcgacc agctcgcgcg caccaagccc aagatgggcg cgaagttcgc
     8101 cgagtggcgc gccctggtca acatcaacaa cacccagccc aacccgttca ccgcgttcga
     8161 ctacgccgcg ccgatcgcga acgtcgacga gatggacgtc accgcgctct acgccaagat
     8221 cggcatgggg tacacggtca cgcaggacgc gatcgacctg gccggcggct acgccgacgc
     8281 gaaggccgtc gcggtgttca acgccctgaa ccagttcaag atcggtgagg acaagggcgg
     8341 tatcggcggc tgcctgttcg ccctgaccac gcccgccacc ccgaccgtct cgcagtccga
     8401 caccggcggc tccatcgccg cgtccaccgc tgtgaacgtg aaggtcgccg cgcgcaccgg
     8461 ggcgaactac ttctggggtg gctccacggt cgcctccgcg cagggcaccg tgaccacctc
     8521 gaccgtggcg gcggccacgc actccgcgac cgccacggtc gccgcggtgc gcggcgcggt
     8581 cgcctacgac tggttcgtcg ccgggttcta ctacacgacc accaccgtga acacggtcac
     8641 gatcacctcg atcccggtcg ccaaccaggc cgtgccgaac ctgccggaca tctacggcac
     8701 cgccccggcc gcggtgccgg tcgccgactc gtcggcgggc gcgaaccagt tcaacggcct
     8761 gctggcgacc ctcgcgggcg actacgccac cgggggcgcc accggcctgg tcacccccgg
     8821 ttcgggcgtc acctcgggcg cgtacttcca ctcgctggac ggcgccgcgt tcaccgtctc
     8881 cggtgcgtcg gtcaaggaac tcgacgacct gaaccagtcg atctacgaca cggtgcgcct
     8941 gtcccccgac gcgtacatga tgtcctcgca gcagggcacc gaactgtcga acgtgatcct
     9001 gtcgaccacc agctcggaga ccttcttcca gccgaacctg gccgggcgct ccgaggccgt
     9061 gctcggagcg ttcgtcggct ggtacgtcaa caaggccgcg ggcggcacgc cgatccgcgt
     9121 cgaggtgcac ccgcacatgc cgcccggcac gctgatcgcg cgcaccgacc gggttccgtt
     9181 ccccaacagc aacatcacca acacgctgga gatgcgctgc ctgcgggacc tggccgacta
     9241 cgagtacgcg gcgaaccggc aggcgaacac cgccggcggc ggcccgcgct tcgacggcga
     9301 gacctacgcc aacgagacgc tgatcaacaa ggcgcccgtc gcgatgggcg tcctgcagaa
     9361 catcaagtag tcccctcccg cgcccggccg acctcccgcg ccggccgggc ccggaaccta
     9421 ccgacccgga ggtaccgcca tgcggatcgc cagcacgaac ggcgcgaccg ccctgcaaca
     9481 cgacggcgtc gactacgagg ccgacgagaa cggcgtgttc gacgtgccgc acgaggtcgg
     9541 gcaggtgctg gtgcgcttcc cgcactggct gcccgagcag gagtccgccg cgaaccgccg
     9601 cgcactggcg gcacaggccg aactcgaccc ggccgggctc gcggcgcgag tgcgcgagtt
     9661 ggaggggcag ctcgcctccc ggcagccgag cgagggcgag tccgagcacc tggcgcggat
     9721 cgccgaactc gaagccgagg tggagtccgc gaaggcggcc aagcctgcgg cgaagaagac
     9781 ggctgcggcg aagaaggccg acgacgccgg cgagtgagcg tcatcgtccc gtacacgcgc
     9841 ctgcacccgg cggcggcgat cctgctggac cgtcacgcgc ccgggcacgt gcgcgtcccg
     9901 ctggacccgg ccgacatgtc cgcgtactgg cggctgctgg ccgagcagtg gcacgaaccc
     9961 ggggacctgc tgatcgtcga gcacgacgtg ggtatccacg ccgacgtcgt cgaaggcctc
    10021 accgcgtgcc ctgagccctg gtgcgggcac ccgtaccgga tcggctcaca gttgctcgtc
    10081 tgcctcggct gcacccgctt cagcgccgca ctcaaggcca gcgagccgga cctgctggac
    10141 gtcgtcggcg aggacggtac cggcggccta ccggcgcgcg actggcgacg actggacgtg
    10201 cggctgctcg acgaactgca ccgccgcggc taccggcagc acgagcacca gccggccgtg
    10261 acgcacttcc acaagtaccc gggcgcctag ggggcggtga gcgtgcaacc tgccgtcgcg
    10321 ccgtacgtgc cgacctacgc caccgagacc tcgtacatca cggtcgacga gtacctggcc
    10381 gaaccgaccg gcgtcgatac cagccagctc gtgccggagg gctcggccga cgacaatcgg
    10441 gccgcgcttg cgcgggtgat cgcgcgcgct tcgtcgcagg ccgacagcat ctgccgcaag
    10501 ccgctggcgg ccacggtgga cgtgcagagc ggcgagtacc gcgtccgcgc ggacggcacg
    10561 atctgggttc cggtggacta tgcgccgatt gtgcaggtca acgcgatctc gctcggctgg
    10621 tcgcagggcg cgatggcggc gatgaccgac ctttccggcg tgcgcgtcga gcgcaagctg
    10681 gtgaagatcc cggttgcggg cgccggagcg tcgtggaacg gctcgccgcg cgtatcgtcc
    10741 cggcgcggct tcatcttcgc ggacgtgacc tacgtcaacg gctgggccgt gaccgctacc
    10801 tcgcaggcct cgctcgcagg cgcgtcatcg ctaagcctgg ccgggtcgct gggcatcgcg
    10861 gccggcctgc gcctgtcgat cgccgacggc gcgaacacgg agagcacgac cgtcggcgcc
    10921 tcgtacgtgc cgggctcgct cacggtgccc accgcggcgc cactgctgta cgcccacggc
    10981 gcgggcgtgt ccgtctcggc gctgccgggc gcgggcaagg acgcggtgat cctgctgacc
    11041 tcgtcgcgga tcaagctgcg cggcgccgag gctgtgcaga tgggctcgat cagtgccgag
    11101 ccgtccgcgc cgcagccgac gatgcccggc ggcaccagcg agtacaacca ggccgtcgag
    11161 ctgctcaagc cgctgcgacg ggcgcggtga gccgcgcgac ggtgcgcgcc gcgctctacg
    11221 cgtacctcca ggccggatgc gccgggggcg gcagcgtacc gggcgtcacg aaggtgtacc
    11281 gcgcgcagcc gtggctgatc gacggcaaga cctggaacct gagcagcgac ctgggctcgg
    11341 gcgccgtggt gttcctgcac ttcatggaca agggcgagac ccggcaagcc gacccggcgg
    11401 cgagcgtgag caccgaagtg gtcggctgga agcaggtcga ctaccaggtc gcgctcgtcg
    11461 tgctctacca gtacctgatc ccgtcgaaca cgcagagcgt gcagtacacg ggcgaggaat
    11521 gggtggatgc gctcgacgcc accctcggcg gcctcgaaga cctgctccgc gcggacccga
    11581 ccgcgggcac cgggccgggt ggcgcggtgt tcgagatggc gcagtccccg ggcgacctga
    11641 cgctgccgca ggacctgccg cgcctggtca acggcaaggt gatgtcctgg caggcgctgc
    11701 atttcacggc caccgagatg atccaggcct gatcacgatc cccgccccgc gcgagctcgg
    11761 gtccgcgcgg ggcggtccct tttcccgcgc cggttcggcg ctgagccgat ggagggccga
    11821 tggccacgac cgatgccgcg cccgcgggcg atggcctgcc gggcggcgg
//
LOCUS       DATCRO010000700         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_97642, whole genome shotgun sequence.
ACCESSION   DATCRO010000700 DATCRO010000000
VERSION     DATCRO010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97642"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..999
                     /locus_tag="VIN75_18990"
     CDS             259..999
                     /locus_tag="VIN75_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976309.1"
                     /translation="MDEEKKAARRTPDRRCTCERALVCNRPARHRFFVGLSMISSSTV
                     TAMSAPPVAGGVLKRLHKLLTRPIRLERRGFDFHFVFDKPPRATGTHPGDESAPRRRN
                     NPGELPSMTAREVSEVCANLRAMLGGQIGRHPRLPSLALLERAMMKNGERGIDDVPAA
                     VLRHAAQALDAFDLDVYGPGLVLLRRRVEQVLRRRHGDRHSHAGHSQPDQHTERLAAA
                     MAPGALKDFSDSMTEFISIDGMFGDRRS"
     gene            complement(1091..1549)
                     /locus_tag="VIN75_18995"
     CDS             complement(1091..1549)
                     /locus_tag="VIN75_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8976310.1"
                     /translation="MATAKKTPAPAKKATKAAAPAKTAKKAAPAKAAKAVKAAPAKAA
                     PAALKPLKTAFNKSSLAAHVAEQSGVDAKSTKAVLAALEGAILMSVNKKGLGSFTLPG
                     LLKVVVHNVPAKKKRMGKDPFTGVERMFAAKPATVKIKTRALKKLKDAAL"
BASE COUNT          301 a          690 c          662 g          348 t
ORIGIN      
        1 cccgccgaca ggccgggtcc tgcggcacgc tccgcctgtc ccagcgggta tcgtgaggcg
       61 atacccgtca cccgggcggg gcctgtggca agactgccca gggtcacgcc gggggtgggc
      121 acccccgcga cgcgtcgcgg cccggtgagt gctcgccgac acgttctccc atatcgcgaa
      181 atgccggaaa tcagctccgc catgccgtaa cgagaacttg caatttggaa gctcgacagc
      241 gttcatccga acgccaaaat ggacgaagaa aagaaggcag cccgccggac tcccgaccgt
      301 cgctgcactt gtgagagggc gcttgtttgc aaccggcctg caaggcatcg ctttttcgtt
      361 gggctcagca tgatctcctc ctctaccgtt acggccatgt ccgctcctcc cgttgccggc
      421 ggggtcctga agcgtctgca caagctgctg acgcgaccga tccgcctcga acgccgtggt
      481 ttcgacttcc acttcgtgtt cgacaagccg ccgcgcgcga ccggcaccca tccgggggac
      541 gaatccgcgc cgcgccgccg caacaacccg ggcgagctgc cttcgatgac ggcgcgtgaa
      601 gtctccgagg tctgcgcgaa cctgcgcgcg atgctcggcg gccagatcgg acgccatccg
      661 cggctgccgt cgctggcgct gctggagcgc gcgatgatga agaacggcga acgcggcatc
      721 gacgacgtcc ccgccgccgt gctgcgccat gcagcgcagg cactggacgc gttcgacctc
      781 gacgtctacg gccccggcct cgtcctgctg cgccgccgcg tcgagcaggt gctgcgccgc
      841 cgccacggcg accgccattc gcacgccggc cactcgcagc ccgaccagca caccgagcgc
      901 ctggccgccg cgatggcccc cggcgcgctg aaggacttct ccgacagcat gacggagttc
      961 atcagcatcg acggcatgtt cggcgaccgc aggtcctgaa agcagcgtcg atcccgccat
     1021 gaaaaagagc cgcgtcctgc ggctctttgc atttggggcg ggcccgtcgg cccgccttcg
     1081 tcggcgctga ttacagcgcg gcgtccttca gcttcttcag ggcgcgggtc ttgatcttca
     1141 cggtcgcggg cttggcggcg aacatgcgct ccacgccggt gaacgggtcc ttgcccatgc
     1201 gcttcttctt ggccggcacg ttgtgcacga cgaccttgag caggcccggc agcgtgaagg
     1261 agccgaggcc cttcttgttg accgacatga ggatcgcgcc ttcgagcgcg gccagcacgg
     1321 ccttggtcga cttcgcgtcg acgcccgact gctcggccac gtgagcggcc aggctggact
     1381 tgttgaacgc cgtcttcagc ggcttcagcg cggccggagc agcctttgcc ggagcggcct
     1441 tcacagcctt cgcggccttc gccggagcag ccttcttggc ggtcttggcc ggcgcggcgg
     1501 ccttggtcgc cttcttggcg ggggcggggg tcttctttgc agttgccatg gttcttcagt
     1561 actttgaact ttggatgtcg atgctgcggc gattgccgca gcggcgcgat tgtaggtgag
     1621 cgccggcatc atccgtgcgg gacagcgagt tttcctgcga tttcctgcac gtgcagcgtg
     1681 atcgtgcata ccctgtgccg tatgcaggcc gtcgcgcggg cgcgtccggc atgtcgccgc
     1741 atcttcgcaa gatccgtcag gccgatcgca atccgaggca gaaggcccgg cgctcgcgag
     1801 cccagaattt ctccacgaca acgatgatgg agacaagtga tgcgaacgaa gaacgagccc
     1861 cgacgtggag tgggaatgcg gtggacgcgc gcggcgagat tcccccaaat cgcaagtgcc
     1921 ggccggcgtg cggcggccac ctcgatgctc ggcgtcgccg tgctcgcgct gggcgcctgc
     1981 ggcggcctgc gaacggcgcc g
//
LOCUS       DATCRO010000701         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_199027, whole genome shotgun sequence.
ACCESSION   DATCRO010000701 DATCRO010000000
VERSION     DATCRO010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199027"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VIN75_19000"
     CDS             complement(<1..774)
                     /locus_tag="VIN75_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8976311.1"
                     /translation="MGRVSLRKVRKSFGTVDVIKDIDLEVEDGEFMVFVGPSGCGKST
                     MLRMIAGLEAIDGGDLLIDGQRANDIRPADRGAAMVFQSYALYPHMTVAENMGFALKM
                     AGVGRKERDEQVRRAADVLRIGDLLGRMPKELSGGQRQRVAIGRAIVRKPRVFLFDEP
                     LSNLDAALRVNMRIELSRLHRELGTTMIYVTHDQVEAMTMGTRIAVFNQGRIEQLGAP
                     LSLYNQPANEFVAAFLGAPRINLVDVPGSDATPVHRALWG"
     gene            complement(777..2132)
                     /locus_tag="VIN75_19005"
     CDS             complement(777..2132)
                     /locus_tag="VIN75_19005"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8976312.1"
                     /translation="MPNDINDLASRFPRDFAWGVATSAFQIEGAAFEDDKGESIWDRF
                     CREPGVIADASNGDVACDHYHRVEDDLDLIAGLGVDLYRFSISWPRVQPAGEGAWNDK
                     GLAFYDRLVDGLLRRGLAPHVTLNHWDLPQALQDKGGWASRDTVHRFVDYALGIQRRL
                     GDRLAAITTHNEPWVVAWLGHKEGTFAPGIKSRKVANQVAHHLLVSHGLALRALRAEG
                     ATARLGIVLNMAHTMPLTGSPEDAAVAKLDDALGRRWYADPLFKGEYPAEALAEFGDD
                     APQVEPGDMAVIATPLDFLGINYYSRHLSSAKGPLAPEDKPYPKTDMGWEIYPDGLRE
                     LLLTMHRDYDLPATYITENGGAFPDPVGADGRVHDADRTDYLATHVRAVGEALAQGVP
                     MAGYMVWSLMDNFEWASGYAKRFGIVHVDYGTLARTPKDSALWFRDLVAQCRALRKTG
                     A"
     gene            complement(2135..3001)
                     /locus_tag="VIN75_19010"
     CDS             complement(2135..3001)
                     /locus_tag="VIN75_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8976313.1"
                     /translation="MSAAATPLPAIGCESAGARTTRWMAYLIVAVGALFMLAPFWFMF
                     VFATHSRSEIFNLPPPLWFGDDLIANFKLLTERLPFWRNLGWSVYVALMATVLTLLFC
                     SMAGYAFALMEFRFKKPLFGLVMATMLLPQFMNMIPMFMIMDALGWIDQQRALYVPGA
                     ASAFGIFLMRQFAASAVPRELVEAARMDGCGEFSIYWRIVLPLLKPALGTLALITFIQ
                     SWNNFINPLIVMRSLPNYTLPLALRSLQSTMNTEWGALMCGSAIATIPLIVLFAITSR
                     QLIAGLTAGAVK"
     gene            complement(3060..3968)
                     /locus_tag="VIN75_19015"
     CDS             complement(3060..3968)
                     /locus_tag="VIN75_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8976314.1"
                     /translation="MRLPSLLRLSPKWAPYVFISPFLVLFGVFGIFPLAFSLYLAFQS
                     WEPTGGLKAMEFVGLANFAFALKDPWFWTSLKNTLWLAVASGVPQHLIALPLAAFIHS
                     SFKRLRDGVIAVYFLPYITSTVAIAIMFNALFSKDYGLINAALAQAFGMEHIDWLHDV
                     AHIKPAIAFIVFWRYVGFNTVLYLAAMQTIPGELYEAATLDGAARWRQFFHITLPSIK
                     PMIFFGVTLSVIGGLQLFEEPFILTGGKGGPQQSAMTSAVYMFREAFNFNDFGGASAM
                     SWLLFLVVALLTWITNLAFRQRKGRA"
BASE COUNT          663 a         1346 c         1407 g          608 t
ORIGIN      
        1 gccccacagc gcgcggtgca ccggcgtggc gtcgcttccg gggacgtcga cgagattgat
       61 gcgcggcgcg ccgaggaagg cggcgacgaa ctcgttggcc ggctggttgt acagcgacag
      121 cggcgcgccg agctgctcga tgcggccctg gttgaagacc gcgatgcgcg tgcccatcgt
      181 catcgcctcg acctggtcgt gcgtgacgta gatcatcgtg gtgccgagct cgcggtgcag
      241 gcgcgacagc tcgatgcgca tgttcacgcg cagcgccgcg tcgaggttgg acagcggctc
      301 gtcgaacagg aagacgcgcg gcttgcgcac gatggcgcgg ccgatggcca cgcgctggcg
      361 ctggccgccg gacagctcct tgggcatgcg ccccagcagg tcgccgatgc gcagcacgtc
      421 ggccgcgcgg cgcacctgct cgtcgcgctc cttgcggccg acgccggcca tcttcagcgc
      481 gaaacccatg ttctccgcca ccgtcatgtg cgggtacagc gcgtagctct ggaacaccat
      541 cgcggcgccg cggtcggcgg ggcggatgtc gttggcgcgc tggccgtcga tcagcaggtc
      601 gccgccgtcg atggcctcca ggcccgcgat catgcgcagc atcgtcgact tgccgcagcc
      661 cgaaggcccg acgaagacca tgaactcgcc gtcctcgacc tcgaggtcga tgtccttgat
      721 gacgtcgacc gtgccgaagg acttgcgcac cttgcgcagg ctcacgcgtc ccatgttcag
      781 gctcccgtct tgcgcagcgc gcggcattgg gcgaccaggt cgcggaacca gagcgcgctg
      841 tccttgggcg tgcgcgccag cgtgccgtag tcgacgtgca cgatgccgaa gcgcttggcg
      901 tagccgctgg cccactcgaa gttgtccatc aggctccaga ccatgtagcc ggccatcggc
      961 acgccctgcg ccagcgcctc gccgaccgcg cggacgtgcg tggccaggta gtcggtgcga
     1021 tcggcgtcgt gcaccctgcc gtcggccccg accgggtcgg ggaacgcgcc gccgttctcg
     1081 gtgatgtagg tggccggcag gtcgtagtcg cggtgcatcg tcagcagcag ctcgcgcagg
     1141 ccgtcggggt agatctccca gcccatgtcg gtcttcggat agggcttgtc ctcgggcgcg
     1201 agcgggccct tggcgctgga caggtggcgc gagtagtagt tgatgcccag gaagtccagc
     1261 ggcgtggcga tgacggccat gtcgcccggc tcgacctgcg gcgcgtcgtc gccgaactcg
     1321 gccagcgcct cggccgggta ctcgcccttg aacagcggat cggcatacca gcggcggccc
     1381 agcgcgtcgt ccagcttcgc gacggcggcg tcctcgggcg agccggtcag cggcatcgtg
     1441 tgggccatgt tcaggacgat gcccagcctg gccgtggcgc cctcggcccg cagcgcgcgc
     1501 agcgccagtc cgtggctgac cagcaggtgg tgcgcgacct ggttggcgac cttgcgcgac
     1561 ttgatgccgg gcgcgaacgt gccttccttg tggcccagcc aggccaccac ccagggttcg
     1621 ttgtgcgtgg tgatcgcggc caggcggtcg cccagccggc gctggatgcc cagcgcgtaa
     1681 tccacgaagc ggtgcacggt gtcgcgcgag gcccagccgc ccttgtcctg cagcgcctgc
     1741 ggcaggtccc agtggttgag cgtgacgtgt ggcgcgaggc cgcgccggag caggccgtcg
     1801 accaggcggt cgtagaacgc caggcccttg tcgttccatg cgccttcgcc cgccggctgc
     1861 acgcgcggcc aggagatcga gaagcggtag aggtccacgc ccaggccggc gatcaggtcg
     1921 aggtcgtcct ccacgcggtg gtagtggtcg caggcgacat cgccgttgct cgcgtcggcg
     1981 atcacgcccg gctcgcggca gaagcggtcc cagatcgact cgcccttgtc gtcctcgaag
     2041 gccgcgccct cgatctggaa ggcgctggtg gccacgcccc atgcgaaatc gcgcgggaac
     2101 cggcttgcaa gatcgttgat gtcgttgggc atgactactt cacggcgccg gcggtcaatc
     2161 cggcgatcag ttggcgcgac gtgatcgcga acaggacgat gagcgggatc gtggcgatgg
     2221 ccgagccgca catcagcgcg ccccactcgg tgttcatcgt cgactgcagg ctgcgcagcg
     2281 cgagcggcag cgtgtagttg ggcaggctgc gcatcacgat cagcgggttg atgaagttgt
     2341 tccaggactg gatgaacgtg atcagcgcca gcgtgcccag cgcaggcttg agcaggggca
     2401 gcacgatgcg ccagtagatg gagaactcgc cgcagccgtc catgcgcgcc gcctccacca
     2461 gttccctggg caccgccgag gcggcgaact ggcgcatcag gaagatgccg aaggcgctgg
     2521 ccgcgcccgg cacgtacagc gcacgctgct ggtcgatcca gcccagggcg tccatgatca
     2581 tgaacatcgg gatcatgttc atgaactgcg gcagcagcat cgtggccatc acgaggccga
     2641 acagcggctt cttgaagcgg aactccatca gcgcgaacgc gtagccggcc atcgagcaga
     2701 acagcagcgt cagcacggtg gccatcagcg ccacgtagac gctccagccg aggttgcgcc
     2761 agaacggcag gcgctcggtc agcagcttga agttcgcgat caggtcgtcg ccgaaccaca
     2821 gcggcggcgg caggttgaag atctcgctgc gcgaatgcgt ggcgaacacg aacatgaacc
     2881 agaacggcgc cagcatgaac agcgcgccca cggcgacgat gaggtacgcc atccagcgcg
     2941 tggtgcgcgc gccggcgctc tcgcacccga tggccggaag gggggtcgcg gctgcgctca
     3001 tggtgaaacc tccgcgctcc gcgctgcggt caattcgccc ttcgggcggc cgtgcgggct
     3061 catgcgcgcc cctttcgctg gcggaaggcc aggttcgtga tccacgtcag cagcgccacg
     3121 accaagaaca gcagccagga catcgcgctc gcgccgccga agtcgttgaa gttgaaggcc
     3181 tcgcggaaca tgtacacggc cgacgtcatc gccgactgct gcgggccgcc cttgcccccg
     3241 gtcaggatga acggctcctc gaacagctgc aggccgccga tcacgctcag cgtcacgccg
     3301 aagaagatca tcggcttgat gctgggcagc gtgatgtgga agaactgccg ccagcgcgcc
     3361 gcgccgtcca gcgtcgcggc ctcgtagagc tcgcccggga tcgtctgcat cgccgcgagg
     3421 tacagcaccg tgttgaagcc cacgtagcgc cagaacacga tgaacgcgat cgccggcttg
     3481 atgtgcgcca cgtcgtgcag ccagtcgatg tgctccatcc cgaaggcctg ggcgagcgcc
     3541 gcgttgatca gcccgtagtc cttggagaac agcgcgttga acatgatcgc gatcgcgacc
     3601 gtcgacgtga tgtatggcag gaagtacacc gcgatcacgc cgtcgcgcag gcgcttgaac
     3661 gaggagtgga tgaacgcggc gagcggcagc gcgatcaggt gctgcggcac gcccgaggcg
     3721 accgccagcc acagcgtgtt cttcagcgac gtccagaacc acgggtcctt cagcgcgaac
     3781 gcgaagttgg ccaggccgac gaactccatc gccttcaggc ccccggtggg ctcccatgac
     3841 tggaacgcga ggtacagcga gaaagccagc gggaagatgc cgaacacgcc gaacagcacg
     3901 aggaacgggc tgatgaacac gtacggcgcc cacttgggcg acagccggag caaggatggc
     3961 agtctcatga tcagcggcga gcgcgcagct cgaggagtcg cgccacgtcg gacagcgcct
     4021 tgcg
//
LOCUS       DATCRO010000702         7823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_165401, whole genome shotgun sequence.
ACCESSION   DATCRO010000702 DATCRO010000000
VERSION     DATCRO010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165401"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VIN75_19020"
     CDS             complement(<1..924)
                     /locus_tag="VIN75_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8976315.1"
                     /translation="MSIERSTELLRLALPWMSRQRAGLHPVSYAVWFDYVAQTNPPLR
                     AAIDEHLARDGALDEFATHALFTRHVADVDPDTAQRVVDQMQRVLGGMGESAGAAGAH
                     ATRYAATLAQLAAALARGDTPRGIDAAAGVARNGPAPRQLADVVAEVIADTGTMRSQI
                     GGLQQRLADSRREIERLRDEVRRAREDALRDSLTGLSNRRAFDQALAALLGAPASVSP
                     AVGARDARAAIDAAKAPAASRPPADAPAPLSAGDGRGPWLLVADIDRFGRVNEAHGRE
                     FGDQVIKAVAEALRTLVPAGATLARVDGEAFG"
     gene            complement(1195..1605)
                     /locus_tag="VIN75_19025"
     CDS             complement(1195..1605)
                     /locus_tag="VIN75_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY8976316.1"
                     /translation="MATATKKAAPAKKAPAKKAAPAKKAAPAKKAAPAKKAAPAKKAA
                     PAKKAAPAKKRTPNAAFMKAMTPSPTLAAVIGSTPIPRTEVTKKVWEYIKKNGLQDKA
                     KRTMINADAKLKEIFKKAQVSMFEMTKLINSHLS"
     gene            2093..2443
                     /locus_tag="VIN75_19030"
     CDS             2093..2443
                     /locus_tag="VIN75_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8976317.1"
                     /translation="MKLIVRWLLLAAALLLVAHLSPGVSVNSFGAALVAALVLGLLNI
                     LVRPLLVLLTLPVTVLTLGLFLFVINALMFWAAAGLLSGFHVDGFGSALIGSVIYSIA
                     STIIDVAIEHLFEE"
     gene            complement(2463..4115)
                     /locus_tag="VIN75_19035"
     CDS             complement(2463..4115)
                     /locus_tag="VIN75_19035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8976318.1"
                     /translation="MRLKFATLAAAACLALNVFVVPHAPAAQPNLPALGEAESDEFSI
                     DDERKVGEQIMRQIRPDPAVIDDPVLLEYLMSIFGPLHEAALKRGNISQEQEEKLAWE
                     PFLVRDTEFNAFALPGGYMGVNLGLIAQAGTRDEMASVIGHELSHITQRHIARSMVAN
                     KHQSMVTMAAMLLGLLFATRSRNGDVPMAVVTSGQAAMATGQLTFSRQMETEADTFGQ
                     DVMTDAGFSPSGMADMFGRLEDVSRLNDSNQYPWLRSHPLTIERIAAARLRARQGQPQ
                     DPAKTHITEHSLMRARARALMDTSDVALRRMQGQARLNAPLNDAQRLGNLYGGALASI
                     ELRDFASASQMIAQADDVMARHAAAVAAQDNAALPPPAPPAQVVVTPKLYPIEPEVAR
                     DFLLLRVEAAAAKRSAPEIVSAVAALGPDRSRPALMARAEAAVARAAARDPAAGGALQ
                     ESVESLQTWVALHPRDVLAWTLLSQCAEPLGQKLRAVRSEAEAHAAQGDLIGAIDRLR
                     AGQRLSRTGKTDFVEASIIDARLRELESIRRELVKNKEMEPQ"
     gene            4264..4767
                     /gene="moaC"
                     /locus_tag="VIN75_19040"
     CDS             4264..4767
                     /gene="moaC"
                     /locus_tag="VIN75_19040"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY8976319.1"
                     /translation="MNDTPDSPAATGLTHFDAQGQAHMVDVGGKAETRRVARATGTIR
                     MQPATLAAIAGGTAKKGDVLGIARIAAIQGAKRTSELVPLCHPLPLTHVQVEFATDAA
                     ASAVHCTVQAETLGRTGVEMEALTAVQVGLLTIYDMVKAIDRGMVMENIRVLEKHGGK
                     SGSWTAP"
     gene            complement(4823..6640)
                     /locus_tag="VIN75_19045"
     CDS             complement(4823..6640)
                     /locus_tag="VIN75_19045"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEY8976320.1"
                     /translation="MNSPSSLGDRPEIRLTAYVLALLCPPLLAWNRTPSATQLNELLC
                     VLCWGLCLLAANALPTTGLRRAPATWLVLPLGIMGLAVVGSWTLGSLPTSLALAPLLS
                     LVVACFVAALGARSSQGGERDGLEAFAPFAFGMVGVGALSAVIAILQVYFPNLCDGTV
                     IAQSGIPGRAVGNLRQPNHLASLLVWSVIALVPLVEWGRIPRWLGALLLALIMWGVFL
                     SGSRTGLWGGALVLLLWVAADSIVSLLLPDPSPLPSMSVSTRVALVVACAIALAAPWL
                     TSHLGHSVVGTATRANDGDSSHFAIWRNTVEMIRQQPWLGVGWGEFNFAWTLTPFPHR
                     PIAFFDHTHDLPLQLLVELGIPLGVLVLGLLLWALGLGLVRAWTTTGRAGQGARAASV
                     MVLMIAIHSLDEYPLWYLYFLLPAAWAWGFTLGSGPSSAEPALRGVDRAKPLESGIVI
                     RMGSEGTGLRVFGGAMIVVAMLCWWDYMERVSTIFLDEPGLPPLAERIQTGETSPLFA
                     HHADYAEATTTEPPSRALGALSRSTHSLLDSRLMIAWATALAESGQNERARYLVARLK
                     EFHKEEADEFFAPCADDTLKVKPFQCQPEPTGLSWRDFR"
     gene            complement(6747..>7823)
                     /locus_tag="VIN75_19050"
     CDS             complement(6747..>7823)
                     /locus_tag="VIN75_19050"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEY8976321.1"
                     /translation="PYVALVLMAAWGAADRQLGRPLRIALVVSPVVAVIMSWGVHVVA
                     NALGILAAVTDRSDAADITSFRSEIWAQSLQLVKAQPWLGVGWGDFNLAWTLTPFGAR
                     SAGLVDNAHNLVLQLAVELGVPLALLMTGLLLAALVLAARRTWRLAGDAGLGARAALM
                     IVVVIGVHSMLEYPLWYAYLLLPTAWAFGFAIGAGKAAGSPAGAAPAAPLRAWRWVGA
                     LLVVIAPSAWLDYLNIVKLFLPDFTSLPLEQRIARAQASPLYANQADYVAVTNLPLTP
                     DTAPLVARSSHVLLNGRLMFLWANLLQAQGQADKARFMAARLREFNLSGPRPWFAPCD
                     DAAVTAKPFQCLQPEHPVTWRDFR"
BASE COUNT         1142 a         2775 c         2790 g         1116 t
ORIGIN      
        1 gccgaacgcc tcgccgtcca cccgcgccag cgtcgccccc gcgggcacga gcgtccgcag
       61 cgcctccgcc acggccttga tcacctggtc gccgaactcg cgcccgtggg cctcgttgac
      121 gcgtccgaag cggtcgatgt cggccaccag cagccacggg ccacggccgt ccccggccga
      181 cagcggcgcc ggcgcatcgg ccggcgggcg gctggcggcg ggcgccttgg cggcgtcgat
      241 ggccgcgcgg gcgtcgcgcg cgccgacggc gggcgacacc gacgccggcg ctccgagcag
      301 cgcggccagc gcctggtcga aggcgcgccg gttcgacagg ccggtcagcg agtcgcgcag
      361 cgcgtcctcg cgggcgcggc gcacctcgtc gcgcaggcgc tcgatctcgc gccggctgtc
      421 ggccaggcgc tgttgcaggc cgccgatctg gctgcgcatg gtgccggtgt cggcaatcac
      481 ctcggccacg acgtcggcca gctggcgcgg cgccgggcca ttgcgcgcga cgccggcagc
      541 ggcgtcgata cccctgggtg tatctccgcg ggccagcgcg gccgccagct gggccagcgt
      601 ggccgcgtag cgggtggcat gggcacccgc ggcgccggcc gattcgccca tgccgccgag
      661 cacgcgctgc atctggtcga ccacccgctg ggcggtgtcg gggtcgacgt cggccacgtg
      721 gcgggtgaac agcgcgtggg tggcgaactc gtcgagcgcg ccgtcgcgcg ccaggtgctc
      781 gtcgatggcg gcgcgcagcg gcggattggt ctgggcgacg tagtcgaacc agacggcata
      841 gctgacggga tgcaggccgg ctcgctggcg cgacatccag ggcaaggcga gccgcagcag
      901 ctcggtgctg cgctcgatgc tcatctcgta tctcaatccg acctcggctg gtttcatgaa
      961 gggcgcggcc tcgattcgca tcggaggaca aatggacggg ccgcactcct gtcgcgcatt
     1021 ctgcccgcgg gtcacgggcg ccgacgggac ttcccgcgcg aacccacgga tttggtgaac
     1081 ggcgcgtgcg cggcgccgcg gtggtccggg cgggcaataa aaaaccggcg cggcgacttg
     1141 cgtcgcggcg ccggttctcg gacatctcgc cagccgccga agcggccgcg gcgatcagct
     1201 caggtggctg ttgatcagct tggtcatctc gaacatcgag acttgcgcct tcttgaagat
     1261 ttccttcagc ttggcgtcgg cgttgatcat cgtgcgcttg gccttgtcct gcaggccgtt
     1321 cttcttgatg tactcccaca ccttcttggt gacctcggtg cgcgggatcg gcgtggagcc
     1381 gatcacggcc gccagcgtcg gcgaaggcgt catcgccttc atgaacgcgg cgttcggggt
     1441 gcgcttcttg gccggagcgg ccttcttcgc gggagcggcc ttcttcgcag gagccgcctt
     1501 cttggccgga gcggccttct tcgcgggcgc cgccttcttg gccggagcgg ccttcttggc
     1561 cggagccttc ttggccggag cggccttctt cgttgcagtt gccatgttga atctctccat
     1621 caagaggttt tgatgtgccg cggtctgggt gagcgtcgca tgaactctcg tttctctgca
     1681 gacatcagcg agacgaatgg tactgcctca tgcagtgcgt gagaagacac ttcacctatg
     1741 aaaaacgcga ttctcacgag ggaaaagcaa gtttttgtct cgcggaggcg tcattcggcc
     1801 ctgcagagaa gcttccagcg cgaatttcta tataccccct gaggtattta tcgaaactcg
     1861 gcgccgcgag tttctcgccg ccgcgcgata ttcgaagcgc ggttttcgcg ccgcggcggc
     1921 gcgcgcatac ccgggttgtc ccgcacgaga cgcggcgcgg gcacgccgcc tgcaggtccg
     1981 ccgccccccc atttcgacgc gctgcggcca gtgcgcgacg gccgcgcggc gtgacggatc
     2041 gcgcgtgctt gccccacaat ccggcgcagt cccaaccaat tgccggttcg tcatgaaact
     2101 catcgtccgc tggctgttgc tggccgccgc gctgctgctg gtggcccacc tgtcgcccgg
     2161 cgtctcggtc aacagcttcg gcgccgcgct ggtggcggcg ctcgtgctgg gcctgctcaa
     2221 catcctggtg cgtccgctgc tggtgctgct cacgctgccc gtcacggtgc tgacgctcgg
     2281 gctgttcctg ttcgtgatca acgcgctgat gttctgggcc gcggccgggc tgctctccgg
     2341 cttccacgtc gacggattcg gatcggcgct gatcggctcg gtgatctact cgatcgcctc
     2401 cacgatcatc gacgtggcca tcgagcacct gttcgaggag tgacgcggcg gcgcgtccgc
     2461 cgtcactgcg gctccatttc cttgttcttg accagttcgc gccggatcga ctcgagctcg
     2521 cgcagccggg catcgatgat cgaggcctcg acgaagtcgg tcttgccggt gcgcgacagg
     2581 cgctggccgg cgcgcagccg gtcgatcgcg ccgatcagat cgccctgcgc ggcgtgcgcc
     2641 tcggcctcgg agcgcacggc gcgcagcttc tggcccagcg gctcggcgca ctgcgagagc
     2701 agcgtccagg cgagcacgtc gcgcggatgc agcgccaccc aggtctgcag cgactcgacc
     2761 gattcctgca gcgcgccgcc ggccgccgga tcgcgcgcgg cggcgcgtgc caccgcggcc
     2821 tcggcgcgcg ccatcagcgc cggacgcgag cgatccgggc ccagcgccgc gacggccgac
     2881 acgatctccg gcgcggagcg cttggccgcc gccgcctcca cgcgcagcag caggaagtcg
     2941 cgcgccacct ccggctcgat cggatagagc ttcggggtga ccaccacctg cgccggcggc
     3001 gccggcggcg gcagcgcggc gttgtcctgc gcggcgaccg ccgcggcgtg cctggccatc
     3061 acgtcgtcgg cctgcgcgat catctgcgag gccgacgcga agtcgcgcag ctcgatcgac
     3121 gccagcgcac ccccatacag gttgcccagc cgctgcgcat cgttgagcgg cgcgttcagg
     3181 cgcgcctggc cctgcatgcg gcgcagcgcg acgtcggagg tgtccatcag ggcccgggca
     3241 cgcgcccgca tcagcgaatg ctcggtgatg tgcgtcttgg ccgggtcctg cggctggccc
     3301 tggcgcgcgc gcagccgcgc ggcggcgatg cgctcgatcg tcaacgggtg cgagcgcagc
     3361 cacgggtact ggttgctgtc gttgagccgg ctgacgtcct ccagccggcc gaacatgtcg
     3421 gccatgcccg acggcgagaa accggcgtcg gtcatcacgt cctggccgaa ggtgtcggcc
     3481 tcggtctcca tctggcgcga gaacgtgagc tggccggtgg ccatcgccgc ctggccgctg
     3541 gtgaccaccg ccatcggcac gtcgccgttg cgcgaccgcg tggcgaacag caggcccagc
     3601 agcatcgccg ccatcgtcac catgctctgg tgcttgttgg ccaccatgct gcgtgcaatg
     3661 tggcgctggg tgatgtgcga cagctcgtgg ccgatcaccg aggccatctc gtcgcgcgtg
     3721 ccggcctgcg cgatcaggcc caggttgacg cccatgtagc cgccgggcag cgcgaacgcg
     3781 ttgaactcgg tgtcgcgcac gaggaagggc tcccaggcca gcttctcctc ctgctcctgg
     3841 ctgatgttgc cgcgcttgag cgccgcctcg tgcagcggcc cgaagatgga catcaggtac
     3901 tcgagcagca ccgggtcgtc gatcacggcc ggatcgggcc ggatctggcg catgatctgc
     3961 tcccccacct tgcgctcgtc gtcgatgctg aactcatcgg actccgcctc gcccagcgcc
     4021 ggcaggttcg gttgcgcggc cggcgcgtgc ggcacgacga agacgttcag cgcgaggcag
     4081 gcggcggcgg cgagggtggc gaatttcagg cgcaagggaa cgatttcctg tccggtcgag
     4141 acgcgggccg gcgaggcccg cggtcgctat gatgacaccg ccatgtatcg gccgtgtagc
     4201 gcatcgttcc tgaagcggac ctgtcgctgc atcgtcggat tcccgccgcc cgcccaaggt
     4261 tccatgaacg acacgcccga ttcccccgcc gccaccggcc tgacgcactt cgacgcccag
     4321 ggccaggccc acatggtcga cgtcggtggc aaggcggaga cgcgccgcgt cgcgcgcgcg
     4381 acgggcacga tccgcatgca gcccgccacg ctcgcggcca tcgctggcgg caccgccaag
     4441 aagggcgacg tgctgggcat cgcgcgcatc gccgcgatcc agggcgccaa gcgcacatcc
     4501 gagctggtgc cgctgtgcca cccgctgccg ctcacgcacg tgcaggtcga gttcgccacc
     4561 gacgccgccg ccagcgcggt gcactgcacc gtccaggccg agacgctcgg ccgcaccggc
     4621 gtcgagatgg aggcgctgac ggccgtccag gtcggcctgc tcaccatcta cgacatggtc
     4681 aaggcgatcg accgcggcat ggtgatggag aacatccgcg tgctggagaa gcacggcggc
     4741 aagtcggggt cgtggacggc gccctgaacc gcgcctgcgg gaaagcagaa ggcccgcatc
     4801 gcgcgggcct tgtccacgga tctcagcgga agtcgcgcca gctgagcccg gtcggctcgg
     4861 gctggcactg gaacggcttg accttgagcg tgtcgtctgc gcacggcgcg aagaactcgt
     4921 cggcctcttc cttgtggaac tccttcagac gggcgacgag gtagcgcgcg cgttcgttct
     4981 ggccgctctc ggccagcgcc gtggcccacg cgatcatcag ccggctgtcg agcagcgagt
     5041 gcgttgaacg cgacagcgcg ccgagcgcgc gcgacggcgg ctcggtggtg gtggcctcgg
     5101 cgtagtcggc gtggtgcgcg aacagcgggc tcgtctcgcc cgtctggatg cgttcggcca
     5161 gcggcggcag gccgggctcg tccaggaaga tggtggagac gcgctccatg tagtcccacc
     5221 agcacagcat cgccacgacg atcatcgcgc cgccgaacac gcgcagcccg gtgccctcgc
     5281 tgcccatgcg gatgacgatg cccgactcca gcggcttggc gcggtcgacg ccgcgcagcg
     5341 ccggctcggc ggacgacggc cccgaaccca gcgtgaaacc ccaggcccac gcggcgggca
     5401 gcaggaagta caggtaccaa agcgggtatt cgtcgaggct gtggatggcg atcatcagca
     5461 ccatgaccga ggccgcgcgt gcgccctggc cggcgcgccc ggtcgttgtc caggcgcgca
     5521 ccaggcccag gccgagcgcc cacagcagca gtcccagcac cagcacgccc agcgggatgc
     5581 cgagttcgac cagcagttgc agcggcaggt cgtgggtatg gtcgaagaag gcgatggggc
     5641 ggtgcgggaa cggcgtgagc gtccacgcga agttgaattc gccccagccc acgcccagcc
     5701 agggctgctg gcggatcatc tcgacggtgt tgcgccagat cgcgaagtgc gagctgtcgc
     5761 cgtcgttggc gcgcgtggcg gtgccgacca cgctgtggcc gagatgggaa gtcagccagg
     5821 gcgccgcgag cgcgatcgcg caggccacga ccagggccac ccgggtgctc acggacatgc
     5881 tcggaagcgg cgacggatcc ggcaggagca acgagacgat cgagtcggcc gcgacccaca
     5941 gcagcagcac cagcgccccg ccccacaggc cggtgcgcga gccgctgagg aacacgcccc
     6001 acatgatcag cgccagcagc agcgcgccca gccagcgcgg gatgcgcccc cattcgacca
     6061 gcggcaccag cgcgatcacc gaccacacca gcaggctggc gaggtggttg ggctggcgca
     6121 ggttgcccac ggcgcgcccg gggatgccgg actgcgcgat gacggtgccg tcgcacaggt
     6181 tggggaaata gacctgcagg atcgcgatca cggcgctcaa cgcgcccacg cccaccatgc
     6241 cgaacgcgaa cggcgcgaac gcctccaggc cgtcgcgctc gccgccttgc gacgaacgcg
     6301 cgccgagcgc cgccacgaag cacgcgacga ccagcgacag cagcggcgcc agcgccagcg
     6361 aggtcggcag cgatcccagc gtccacgagc cgaccaccgc caggcccatg atgcccagcg
     6421 gcagcaccag ccacgtggcg ggcgcgcggc gcaggccggt cgtcggcagc gcgttggcgg
     6481 ccagcaggca caggccccag cacagcacgc acagcagttc gttgagctgg gtggccgacg
     6541 gggtgcggtt ccacgccagc agcggcgggc acagcagcgc cagcacgtag gcggtcaggc
     6601 ggatttccgg acgatcgccg agggaggatg gactgttcat tgcgggactg gaaggtcgac
     6661 ggcgccgcct gcggcgacga aaacggtgca cagggtaacg cgaatcgggc cggacgccgc
     6721 cgccgggcgc tccgcgcgcc ctgcggctac ctgaagtcgc gccaggtcac gggatgctcg
     6781 ggctgcaggc actggaacgg cttggcggtg acggccgcgt cgtcgcacgg cgcgaaccag
     6841 ggccgcggtc cggacaggtt gaactcgcgc agccgcgccg ccatgaagcg cgccttgtcg
     6901 gcctggccct gcgcctggag caggttggcc cacaggaaca tcaggcggcc gttgagcagc
     6961 acgtgcgacg agcgcgcgac cagcggggcc gtgtcgggcg tcagcggcag gttggtgacg
     7021 gccacgtagt cggcctggtt ggcgtacagc gggctcgcct gcgcgcgggc gatgcgctgc
     7081 tcgagcggca gcgacgtgaa gtcgggcagg aacagcttga cgatgttgag atagtccagc
     7141 cacgccgacg gcgcgatcac caccagcagc gcgccgaccc agcgccacgc gcgcagcggc
     7201 gccgccggcg cggcgcccgc cggggacccg gccgccttgc cggcgccgat ggcgaagccg
     7261 aacgcccacg cggtgggcag cagcaggtag gcataccaga gggggtattc gagcatgctg
     7321 tgcacgccga tcaccacgac gatcatcagc gcggcgcgcg cgcccaggcc cgcgtcgccc
     7381 gccaggcgcc aggtgcggcg cgcggccagc accaacgccg ccagcagcag gcccgtcatc
     7441 aacagcgcga ggggcacgcc cagctcgacg gccagctgca gcaccaggtt gtgcgcgttg
     7501 tcgacgaggc cggcgctgcg cgcgccgaac ggcgtcagcg tccaggccag gttgaagtcg
     7561 ccccagccga cgcccagcca gggttgcgcc ttgaccagct gcagcgactg cgcccagatc
     7621 tcgctgcgga aggacgtgat gtcggcggcg tcgctgcggt cggtgacggc cgccaggatg
     7681 cccagcgcgt tcgcgacgac gtgcacgccc cacgacatga tcaccgccac gaccggcgag
     7741 acgaccagcg cgatgcgcag cggccgcccc agctggcgat cggccgcgcc ccacgcggcc
     7801 atgagcacca gcgccacgta ggg
//
LOCUS       DATCRO010000703         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_221456, whole genome shotgun sequence.
ACCESSION   DATCRO010000703 DATCRO010000000
VERSION     DATCRO010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221456"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VIN75_19055"
     CDS             complement(<1..1518)
                     /locus_tag="VIN75_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8976322.1"
                     /translation="MTTATPSDVAESTRIVQRICPLCEACCGLSLTVSGTGEQARVIA
                     IRGDEEDVFSHGYVCPKAIALKDLEDDPDRLRRPLVKRDGRFVETSWDEAYAEIARRL
                     PPLLAAHGKDALAFTLGNPNVHRLGLGLYVQRLLRAAGTRNIFSASTLDQMPRQVASG
                     WMYGHALTVPVPDIDRCDFLLMLGANPMASNGSMWTVPDFRGRAKAMRARGGRLVVVD
                     PRRTETAAIADEHHFIRPGADVFLLLGLVNAVFDEGLVRPGRLEGFLATGETGVEALQ
                     RAVAPYTPERMAARCGVPAEAQRALVRQLAAAPRGAVYSRIGSSTQRFGTLNSVLVDA
                     LNILTGHLDAEGGAMFPQAAAFAANTQGKPGVGRGLVTGRTRSRVSGAPEVLGEYPTS
                     CLAEEIETPGAGQVKALISVASNPVLSAPNGARIAKALAELDFMVSVDIYVNETTQHA
                     DVILPGLSSFEEPHYDVSFPQLSCRNHARYSPPVFAPRPGAQAEWQALLRIAAMLR"
     gene            1731..>3670
                     /locus_tag="VIN75_19060"
     CDS             1731..>3670
                     /locus_tag="VIN75_19060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8976323.1"
                     /translation="MNAVLSDPPRRPHRASLASLHLRLLRAEWGHHPWRQAAAVLAVA
                     LGVALAFAVQLINASALSEFSAAVHAASGAPDFSVRPRDGLLSEDLYPRLAMAPGVAQ
                     ASPVVDLPLTLRGADGQGHPAHLLGLDAFVAPWISPGWLVQPEAAAASGVASPGAPLA
                     ALDPDLVFLNAAARALVGPGQRVTMVVDGRTHEFVVGGRIAADGPPLLVMDIAGAQTH
                     FDRVGSLSRIDVRLAPGARQEAVMAALDAGPGVRAAAPDEAALRMARLSQSYRVNLSV
                     LSLVALFTGAFLVFSVQSLAVAKRIPQLALLGVLGMDGRQRRALVWIDSLMVGVLGGA
                     AGLALGTALAAAALRALGGDLGSGTLGGSAPHLQWSGLAALAYGALGIASAFAGGWWP
                     ARAAQGIAPAQSLKGLSTAAGADWPVAAGLGLIVLAALLALVPPWHAIPWAAYACVGV
                     LLLGGISLVPQVVALLLKGFRGARNPVAALAVARAVDQRHTATAAVAGVVASLSLVVA
                     LTVMVSSFRDSLIAWLDDVLPADLYVREVPGASDTPVFLPPAVVAAASGPGVRQVRAE
                     RLVPVTLAPDQPEAALLVRDLLPGETQPRLPWVVAPDDAPPASSGLLSVYPTEVLAAL
                     QHLKKGDVIPVPLPPPRGAAASP"
BASE COUNT          419 a         1355 c         1370 g          526 t
ORIGIN      
        1 gcgcagcatc gcggcgatgc gcagcagcgc ctgccattcc gcctgcgcgc cgggccgcgg
       61 cgcgaacacc ggcgggctgt agcgtgcatg gttgcggcag gacagctgcg ggaagctcac
      121 gtcgtagtgc ggctcctcga agctggacag ccccggcagg atgacgtcgg cgtgctgcgt
      181 cgtctcgttc acgtagatgt ccacgctcac catgaagtcg agctcggcca gcgccttcgc
      241 gatgcgcgcg ccattgggcg ccgacagcac cgggttgctg gccaccgaga tcagcgcctt
      301 cacctgcccc gcgcccgggg tctcgatctc ctcggcaagg cagctggtcg ggtactcgcc
      361 cagcacctcg ggcgcgcccg acacgcggct gcgcgtgcgg cccgtcacca gcccgcggcc
      421 gacgccgggc ttgccctgcg tgttggcggc gaacgccgcg gcctgcggga acatggcgcc
      481 gccctcggcg tcgaggtggc ccgtgaggat gttcagggcg tcgaccagca ccgagttgag
      541 cgtgccgaag cgctgcgtgc tcgatccgat gcgcgagtag accgcgccgc gcggcgccgc
      601 cgccagctgg cgcaccagcg cgcgctgcgc ctcggccggc acgccgcaac gcgccgccat
      661 gcgctcgggg gtatacggcg cgacggcccg ctgcagggcc tcgacacccg tctcgcccgt
      721 ggcgaggaac ccctcgaggc gacccgggcg caccagcccc tcgtcgaaca ccgcattgac
      781 caggcccagc agcaggaaca cgtcggcgcc cggccggatg aagtggtgct cgtcggcgat
      841 ggcggccgtc tcggtgcggc gcggatcgac caccaccagc cggccgccac gcgcgcgcat
      901 cgccttggcg cggccgcgga agtcgggcac cgtccacatg ctgccgttgc tggccatcgg
      961 gttggcgccc agcatcagca ggaaatcgca gcggtcgatg tcgggcaccg gcacggtcag
     1021 cgcgtggccg tacatccagc cgctggcgac ctggcgcggc atctggtcga gcgtggaggc
     1081 gctgaagatg ttgcgcgtgc ccgccgcgcg cagcaggcgc tgcacgtaca ggcccagccc
     1141 gagccggtgc acgttcgggt tgccgagcgt gaacgcgagc gcatccttgc catgcgcggc
     1201 gagaagcggc ggcaggcgac gcgcgatctc ggcataggcc tcgtcccagc tcgtctcgac
     1261 gaagcggccg tcgcgcttga ccagcggccg gcgcaggcga tcgggatcgt cctcgagatc
     1321 cttcagcgca atcgccttgg ggcagacgta gccatggctg aagacgtcct cctcgtcgcc
     1381 gcggatggcg atgacgcgcg cctgctcgcc tgtgcccgac actgtcaggc tcaggccgca
     1441 gcacgcctcg cacagcgggc agatgcgctg cacgatgcgc gtggattcgg cgacgtcgga
     1501 aggggtggcg gtcgtcatgg cgttgtctcg gggtggtcgt gctcgtctgg cgcaaagcat
     1561 acggcgccgc ggtcgatgat ggggacgccg cgacgccgtg gctgccactt cggcgacaaa
     1621 ccccgggaaa tggcgcgcac ggcgccgaac gggcggttaa cccgccgatc cgcgccggca
     1681 ggaatccgga ttgatggcag gatgccggtg tcccatcgat cgccctgccc ttgaacgccg
     1741 tcctgtccga tccgccgcgg cgcccgcacc gcgcgtccct cgcctcgctg cacctgcgcc
     1801 tgctgcgggc cgagtggggc catcatccgt ggcgccaggc cgcggccgtg ctggccgtgg
     1861 cgctgggcgt cgcgctggcc ttcgcggtgc agctgatcaa tgcatcggcg ctgtcggagt
     1921 tctcggcggc cgtgcacgcg gccagcggcg cgcccgactt cagcgtgcga ccgcgcgacg
     1981 gcctgctgtc cgaggatctc tatccgcggc tggcgatggc gccgggcgtg gcgcaggcca
     2041 gtccggtggt cgacctgccc ctgacgctgc gcggcgccga cgggcagggc catcccgcgc
     2101 acctgctggg cctggacgcg ttcgtggcgc catggatctc gcccgggtgg ctcgtccagc
     2161 ccgaggcggc ggccgcctcg ggcgtggctt cgcccggcgc gccgctggcc gcgctcgacc
     2221 ccgacctcgt cttcctcaac gccgcggcac gcgcgctcgt cggcccgggg cagcgcgtca
     2281 cgatggtcgt cgacggacga acgcacgagt tcgtcgtcgg cggccgcatc gccgccgacg
     2341 gcccgccgct gctggtgatg gacatcgccg gcgcgcagac gcatttcgac cgcgtcggct
     2401 cgctgtcgcg catcgacgtg cgcctggcgc cgggcgcgcg ccaggaggcc gtgatggccg
     2461 cgctggacgc cgggcccggc gtgcgcgccg ccgcgcccga cgaggcggcg ctgcgcatgg
     2521 caaggctctc gcagtcctat cgcgtcaacc tgagcgtgct gtcgctggtg gcgctgttca
     2581 cgggcgcctt cctggtgttc tccgtgcagt cgctcgcggt ggccaagcgg attccccagc
     2641 tggcgctgct gggcgtgctg ggcatggacg gacgccagcg ccgcgcgctc gtctggatcg
     2701 acagcctgat ggtgggggtg ctcggcggcg ccgccggcct ggcgctcggt acggcgctgg
     2761 ccgcggccgc gttgcgcgcg ctcggcggcg acctgggcag cgggacgctg ggcggcagcg
     2821 cgccgcacct gcagtggagc gggcttgccg cgctggccta cggcgcgctg ggcatcgcat
     2881 ccgcgttcgc cggcggctgg tggccggcgc gcgccgcgca aggcatcgcg cccgcgcaga
     2941 gcctcaaggg cctcagcacg gcggcaggcg ccgactggcc cgtcgcggcg gggctgggcc
     3001 tgatcgtgct ggccgcgctg ctcgcactgg tgccgccctg gcacgccatc ccctgggccg
     3061 cctatgcctg cgtgggcgtg ctgctgctag gtggcatcag cctggtgccc caggtcgtcg
     3121 cgctgctgct caaggggttt cgcggcgccc gcaatccggt cgccgcgctc gcggtggcac
     3181 gcgcggtcga ccagcgccac acggccaccg ccgcggtggc gggcgtcgtc gccagcctga
     3241 gcctggtcgt ggcgctgacg gtgatggtca gcagcttccg cgattcgctg atcgcctggc
     3301 tggacgacgt gctgcccgcc gacctctacg tgcgcgaggt gcccggcgcc agcgacacgc
     3361 ccgtgttcct gccgccggcg gtggtggcgg ccgcctccgg cccgggcgtg cgccaggtgc
     3421 gcgccgaacg gctggtgccg gtgacgctcg cgcccgacca gcccgaggcc gcgctgctgg
     3481 tgcgcgacct gctgcccgga gagacgcaac ccaggctgcc gtgggtggtc gcgcccgatg
     3541 acgcgccgcc cgcctcgtcc ggcctgctgt cggtctaccc cacggaggtg ctggccgcgt
     3601 tgcagcacct gaagaagggc gacgtgatcc cggtgccttt gccgcctccg cgcggcgccg
     3661 cggcctcgcc
//
LOCUS       DATCRO010000704        10797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_784216, whole genome shotgun sequence.
ACCESSION   DATCRO010000704 DATCRO010000000
VERSION     DATCRO010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784216"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VIN75_19065"
     CDS             <1..901
                     /locus_tag="VIN75_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8976324.1"
                     /translation="EVPAGTPVAALSDITLAIRAGETVAFVGPSGAGKTSLVNLLPRF
                     VEPSEGRVLIDGVAIADYDITALRSQFALVSQDVVLFNDSVAANVALGATIDPARVRD
                     ALAGANLLEFVQSMPGGLDAQIGHNGNQLSGGQRQRLAIARAIYKDAPILILDEATSA
                     LDSESERAVQAALEVLMKGRTTLVIAHRLSTIEHADRVVAMENGRIVEQGPHAQLLAA
                     GGLYARLHPCSSAAPEPAAASRPRRRDRQATAGVSTTATRPLLSSSRREACSPHPEST
                     ARNSSSSNNRRFFMTISNEACGR"
     gene            complement(1097..3046)
                     /locus_tag="VIN75_19070"
     CDS             complement(1097..3046)
                     /locus_tag="VIN75_19070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8976325.1"
                     /translation="MSGGLRVLVDVARVLPGWARACAAPLALLLAIVVAGLPGAWAAG
                     DAPVAPALPVSQTITRAEFCMMPGLADTMPGASCNWQTVDLAKVWHAPRKDVPLVDAW
                     FKLRFHLDAVPVQGLALYTVAFNRSGRVYVNGSLLRELGAMRDPLPLNWNRAQYIVLP
                     GMMLRAGDNEIEIQQRIHPTEGGWLAPVRLGTEDELRPIWQRRVFWQSDIVRLLGSCT
                     FAIGLFMLGVWFGRRRDWMYFWFGCMALSWSVQSVDSYAEMPPLPPLLWDRVVVCMPV
                     LRGVFMLMFVLSYGSLRRPVVATVAWGYFLLGALAIFSGSMPHAGMDLWFLGFLVASI
                     YILYLQVRVGLKRSRVEGVLLLCAGMQDMALSAHDLVLYSEHTWADRFYLAYYSAPLY
                     LFVIGTILVRRFTESLSAHENLAAVLEQRVDQKAAELERNYEQLVEARRNEALALERT
                     RIMSEMHDGIGSQLTMALSLVRQLDREHDPERAGEDGRVATVLRESIEDLQLIIDSLE
                     PVENDLLTVLGTLRYRLQDRLGKSGIDLHWNVVDLPPLPMLTPHSVLSILRIVQEAFA
                     NCLKHSGADRIAVTTGLQGSPGVDEMAHIVIADNGRGIDLSRGSGRGLENMRRRAAAL
                     GGQMKIQSQAGGTEVRLEFPTLRSA"
     gene            complement(3043..3723)
                     /locus_tag="VIN75_19075"
     CDS             complement(3043..3723)
                     /locus_tag="VIN75_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976326.1"
                     /translation="MPDTVDMPLETPTRILVVDDDDAFRESVCAAIARDGAMVLAAEV
                     GNAERAREAIADGQFDVALVDLGLPDGNGIDLIREIARTRPDADVMVVTVFGDEAHVL
                     ASIEAGATGYLLKRSLNDTLGATVRELRAGGSPISPVIARQLLHRFKKDSPTPSTPAV
                     VDDGGLSEREREVLLFIAKGFTVGEIANMLHLSAHTVATHVKHIYRKLAVHSRTEAVF
                     EAGRMGLL"
     gene            complement(3834..4571)
                     /locus_tag="VIN75_19080"
     CDS             complement(3834..4571)
                     /locus_tag="VIN75_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976327.1"
                     /translation="MAWKHRPDPRAIPGRPDDDAMRQGAAARHAPIDHEKGRPMIAIT
                     VSTNYHDILKLVLEADLKFFDRWIFVTDVDDIQTIDVLTKHDVIVLYHDFRNGGRVFD
                     KGGAVAKAQQFAYRHYPDQWYLLMDSDICLSPEFAALPPAVQGFDPDAVFGAKRRHDF
                     GKLSDYRTGRNYFEYPWGDQLQGYFQLYKKHVLYQSSRDASACDLDFLAHFDKKVTID
                     GFVCNHLGRKGNWQGRKVGEDFEFDVA"
     gene            4662..5504
                     /locus_tag="VIN75_19085"
     CDS             4662..5504
                     /locus_tag="VIN75_19085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8976328.1"
                     /translation="MPLWLQTYLDALTNPGLWRAFRRVYWAALVGDGGRYLACVLLVW
                     GLLHVLLRKRIAHRLISAWPSRADLRREITYSFSSLLVVSAGSAVMLAMVVSGWGQVY
                     HDPLKYGWTWLLLSLPVAIVLHDLYFYWTHRLLHTPWLFRHVHGVHHRSRHPSPWAAL
                     SFHPVEALIQGAPLSLILLVVPLDDYVVFAFLLHQMLRNAYGHGGVELFPRGFAHHWF
                     WGRFVTSTHHHLHHETAQGNYGLWFTWWDRWCGTERAEYLERFDKVTMPRPRPADDGA
                     GAVA"
     gene            complement(5529..6992)
                     /gene="rng"
                     /locus_tag="VIN75_19090"
     CDS             complement(5529..6992)
                     /gene="rng"
                     /locus_tag="VIN75_19090"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY8976329.1"
                     /translation="MHDILVNWSPQETRVAIIENGAIQELHLERALERGLVGNVYLGK
                     VARVLPGMQSAFIDIGLERAAFLHVADLHGHPAAKPENAPAAIEKLVFEGQSLTVQVV
                     KDPIGTKGARLTTQVSIAGRLLVYLPHDNHIGISQRIGSHELREQLRARMIALAGNPL
                     EGGGGFILRTNAEEASDQELAEDIAYLRTTWNRIRERALSKPAGSLLHQDLSLAERVL
                     RDIANEHTQSIRIDSKLQYESLRAFGETYASSSVGRLEHYKGERPIFDLYNIEEEIAR
                     ALARRVDLKSGGYLIIDQTEALTTIDVNTGGFVGARNFDETIFKTNLEAAGAIARQLR
                     LRNLGGIIIVDFIDMTRDEHQQAVLAEFRKQLSKDRTKVTVSGFTGLGLVEMTRKRTR
                     ESLAHMLCEPCPTCAGRGQVKTPRSVCYDVLREILREARQFDPKEFRVIASAAVVEML
                     LDEESAHLADLSAFIRKPISLSAEPTMNPEQYDIVLL"
     gene            complement(7019..7630)
                     /locus_tag="VIN75_19095"
     CDS             complement(7019..7630)
                     /locus_tag="VIN75_19095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8976330.1"
                     /translation="MKHPFIYLASQSPRRRQLLDQLGVRHELLLASPDEDAETLEEHI
                     PGETPAVYCERVTQLKLDAAVARMKKRGLPPAPVLCADTTVALGRRIFGKPADADDAV
                     ATLERLSGRTHRVMTAVALASGRARTSALSVSTVRFAPMPAHAIAAYVASGEPFGKAG
                     AYAIQSRVAAWIERIAGSYSGIMGLPLHETARLLMDAGVEFQE"
     gene            complement(7887..8357)
                     /gene="rlmH"
                     /locus_tag="VIN75_19100"
     CDS             complement(7887..8357)
                     /gene="rlmH"
                     /locus_tag="VIN75_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY8976331.1"
                     /translation="MRLIIASVGQKPPAWAEAAYEEFERRFPPEMRLELKAVKAEPRT
                     SGKIAAQMMQAEAKRLEAVLPAGVRRVVLDERGTRWTTRALADRLEFWRGDGRDVAFL
                     IGGPDGLDPDLKATADETARLSDLTLPHAFVRPLLAEALYRAWSVSTNHPYHRE"
     gene            complement(8417..9289)
                     /gene="rsfS"
                     /locus_tag="VIN75_19105"
     CDS             complement(8417..9289)
                     /gene="rsfS"
                     /locus_tag="VIN75_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY8976332.1"
                     /translation="MDIRKLQRAIVDGLEDVKAQDIQVFNTEHLSPLFERVIVAAGAS
                     NRQTKALAASVRDAVKSAGMDVLRTEGEENGEWIIVDCGAAVAHIMQPSIRDYYRLEE
                     IWGGKPVAIKLGGGRSGGLVKASEGADDDADEAPKPRKRAKAAKVVDADETPARKAAL
                     KKPAGKKTAAGKPAPLPRAKTPAERSSSTRGPAAKTSARAAAATKPAGSQAGKAKPAP
                     KSAAGKVAAAKKTRAGSTAARAGGAPVKAASVKKVVVPANSSRNAATAAKKTARKTAA
                     AKSAVAKKVAARRG"
     gene            complement(9384..9977)
                     /gene="nadD"
                     /locus_tag="VIN75_19110"
     CDS             complement(9384..9977)
                     /gene="nadD"
                     /locus_tag="VIN75_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY8976333.1"
                     /translation="MNAGLFGGSFDPPHLAHRALAQCALEQLALDELVWLPARQSPHK
                     GGRLPASGADRVAMLQRLIEGEPRFSIDARELDRAGPSFTVDTLRALHAEQPGTRWWL
                     VIGQDQYARFDTWHDWRGIVALAGLAVAARDGDPVRAAPGLAGVEHERRIVQMPPHPH
                     SATAVRERAAAGLDVTALVGAPVARYIADHHLYTLPK"
     gene            complement(10084..>10797)
                     /gene="hemF"
                     /locus_tag="VIN75_19115"
     CDS             complement(10084..>10797)
                     /gene="hemF"
                     /locus_tag="VIN75_19115"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY8976334.1"
                     /translation="VKGRTLPPSATAARPELAGAAFEALGVSLVLHPRNPYVPTVHMN
                     VRFFEATADGKDPVWWFGGGMDLTPYYGDEADARHFHATCRDALAPFGETLYPRFKKW
                     CDEYFFLKHRNEPRGIGGIFFDDVNELGFEQGFAMLRSVGDAFLAAYLPIVERRRGLP
                     FGERERDFQAYRRGRYVEFNLVWDRGTLFGLQSGGRTEAILMSMPPLAQWRYQWSPQD
                     GTPEAALYADFLRPRDWLA"
BASE COUNT         1565 a         3717 c         3795 g         1720 t
ORIGIN      
        1 cgaggtgccc gccggcacgc cggtggccgc gctgtcggac atcacgctgg ccatccgcgc
       61 cggcgagacg gtcgccttcg tcgggccgtc gggcgcgggc aagacctcgc tggtcaacct
      121 gctgccgcgc ttcgtcgagc ccagcgaggg ccgcgtgctg atcgacggcg tggccatcgc
      181 cgactacgac atcaccgcgc tgcgcagcca gttcgcgctg gtcagccagg acgtcgtgct
      241 gttcaacgac agcgtcgccg ccaacgtggc gctgggcgcg accatcgacc ccgcgcgcgt
      301 gcgtgacgcg ctcgcgggcg cgaacctgct ggagttcgtg cagtccatgc ccggcggcct
      361 cgatgcgcag atcggccaca acggcaacca gctgtcgggc ggccagcgcc agcggctcgc
      421 gatcgcgcgg gccatctaca aggatgcgcc gatcctcatc ctggacgagg ccacgtcggc
      481 gctcgactcg gagtccgagc gcgcggtgca ggcggcgctg gaggtgttga tgaaagggcg
      541 cacgacgctg gtcatcgcgc accggctgtc gaccatcgag catgccgacc gcgtcgtcgc
      601 gatggagaac gggcgcatcg tggaacaggg gccgcacgcg cagttgctgg cggcgggagg
      661 gctctacgct cgactgcacc catgcagttc cgcggcgcct gagccggccg cggcctcccg
      721 gccgcggcgg cgcgaccgtc aggcgacggc cggggtatcg acgacggcga cgcggccgct
      781 gctttcgtcg tcgaggaggg aagcctgttc gccgcacccg gaaagcacgg ccaggaacag
      841 tagcagcagc aacaatagac gcttcttcat gacgatctcc aacgaggcct gcggacggtg
      901 acgggagggc tcgaccgcgc ggcggactct gatcggcacg ctcgcgctgt ggcagaaata
      961 ccgtcgctag gtatgaagat gctcgccgtt cgattcagtg tccgggtagc ggactttccc
     1021 tgaaactgtg tgcaattgat ggggtgatcg cgttggtcga gcagagatct ccccgaaacc
     1081 ggtgaatcgg ccggtgttag gcagaccgga gcgtggggaa ttcgaggcgg acctccgttc
     1141 caccggcctg ggactggatc ttcatctgcc cgcccagcgc tgccgcgcga cgccgcatgt
     1201 tctccagccc gcggccggaa ccgcggctca ggtcgatgcc gcggccgttg tcggcgatga
     1261 cgatgtgcgc catctcgtcg acgccgggac tgccctgcag gccggtggtc accgcgatcc
     1321 ggtcggcgcc cgaatgcttc aggcagttgg cgaaggcctc ctgcacgatg cgcaggatcg
     1381 acagcacgct gtgcggcgtg agcatcggca gcggcggcag gtccaccacg ttccagtgca
     1441 ggtcgatgcc gctcttcccg agccgatcct gcagccgata gcgcaacgtg cccaacaccg
     1501 tcaggaggtc gttctccacg ggctccagcg agtcgatgat cagttgcagg tcctcgatgc
     1561 tctcgcgcag cacggtggcg acgcggccgt cctcgccggc gcgctccgga tcgtgctcgc
     1621 ggtccagctg gcgcacgagc gacagcgcca tggtcagctg cgagccgatg ccgtcgtgca
     1681 tctcgctcat gatgcgcgtg cgctccaggg ccagcgcctc gttgcggcgc gcctccacca
     1741 gctgctcgta gttgcgctcg agctcggcgg ccttctggtc gacgcgctgc tcgaggacgg
     1801 cggccaggtt ctcgtgcgcg ctgagcgact cggtgaagcg gcgcacgagg atcgtgccga
     1861 tcacgaacag gtagagcggc gcgctgtagt aagcgaggta gaagcggtcg gcccaggtgt
     1921 gctcggagta cagcaccagg tcgtgcgcgc tcagcgccat gtcctgcatg ccggcgcaca
     1981 ggagcaggac gccttccacc cggctgcgct tgagccccac gcggacctgc aggtacagga
     2041 tgtagatcga cgcgaccagg aagccaagga accagaggtc catgcccgcg tgcggcatcg
     2101 agccgctgaa gatcgccagc gcaccgagca ggaaatagcc ccaggcgacc gtcgccacca
     2161 ccggacgccg caggctgcca tagctgagca cgaacatgag catgaacacg ccgcgcagca
     2221 ccggcatgca cacgaccacg cgatcccaca gcaacggcgg cagcggcggc atctcggcgt
     2281 aggaatcgac gctctgcacc gaccaggaca acgccatgca gccgaaccag aagtacatcc
     2341 agtcgcgccg gcgcccgaac cagacgccga gcatgaacag cccgatcgcg aaggtgcagg
     2401 aacccagcag ccgaacgatg tcgctctgcc agaacacgcg ccgctgccag atcgggcgca
     2461 gctcgtcctc ggtgcccagg cgcacgggcg cgagccagcc gccctcggtc ggatggatgc
     2521 gctgctggat ctcgatctcg ttgtcgccgg cgcgcagcat catgccgggc agcacgatgt
     2581 actgggcgcg gttccagttc agcggcagcg gatcccgcat ggcacccagc tcgcgcaaaa
     2641 ggctgccgtt gacgtacacg cgccccgacc ggttgaacgc gacggtgtac agggccagcc
     2701 cctggaccgg caccgcgtcc agatggaagc ggagcttgaa ccaggcgtcg accaacggca
     2761 cgtccttgcg gggagcgtgc cacaccttgg cgaggtcgac ggtctgccag ttgcacgagg
     2821 cgccgggcat cgtgtcggcc aggcccggca tcatgcagaa ctcggcccgc gtgatcgtct
     2881 gcgacaccgg cagcgcaggc gcgaccggag cgtctccggc cgcccacgcg cccggcaagc
     2941 cggcgacgac gatcgccagg agcagcgcca gcggcgccgc gcacgcgcgc gcccagccgg
     3001 gcagcacgcg ggcgacgtcg acgaggacgc gcaggccccc gctcacagca ggcccatgcg
     3061 gcccgcctcg aacacggcct cggtgcgcga atgcaccgcg agcttgcgat agatgtgctt
     3121 cacgtgcgtg gccaccgtgt gcgccgacag gtgcagcatg ttggcgatct cgcccaccgt
     3181 gaagcccttg gcgatgaaca ggagcacctc gcgctcgcgc tcggacagtc cgccatcgtc
     3241 caccacggcc ggtgtggacg gcgtggggga gtccttcttg aagcggtgca gcaactggcg
     3301 cgcgatcacc gggctgatcg gcgacccgcc cgcgcgcagt tcccgcacgg tggcgcccag
     3361 cgtgtcgttg aggctgcgct tgagcaggta gccggtagcg cccgcctcga tcgaggcgag
     3421 cacgtgggct tcgtcgccga acacggtgac caccatcacg tcggcgtccg gccgcgtgcg
     3481 ggcgatctcg cggatcaggt cgatgccatt gccgtccggc aggccgaggt cgaccagcgc
     3541 gacgtcgaac tggccgtccg cgatcgcctc gcgcgcgcgc tcggcgttgc cgacctcggc
     3601 ggccagcacc atggcgccgt cgcgggcgat ggcggcgcag acggactcgc ggaaggcgtc
     3661 gtcatcgtcc acgaccagga tccgggtcgg ggtctcgagg ggcatgtcaa cggtatcggg
     3721 catcgcttca ttggagcagc acagccccga ctgtaaacgc ccacatgcat ccccggtcca
     3781 gggtattgca ccggtctgcc ccgccgatcg cccgatttgg acgccttctc gcgtcaggcg
     3841 acgtcgaatt cgaagtcctc gccgaccttg cggccctgcc agtttccctt gcggcccagg
     3901 tgattgcaga cgaatccgtc gatcgtcacc ttcttgtcga aatgcgcgag gaagtcgagg
     3961 tcgcacgcgc tcgcgtcgcg cgacgactga tacagcacat gcttcttgta gagctggaaa
     4021 tagccttgca gctggtcgcc ccagggatat tcgaagtagt tgcggccggt gcggtaatcg
     4081 ctgagcttgc cgaaatcgtg gcgtcgcttg gcgccgaata ccgcgtccgg gtcgaaaccc
     4141 tgcaccgcgg gcggcagcgc ggcgaattcg ggcgacaggc agatgtcgga atccatcagc
     4201 aggtaccact ggtcggggta atggcgatag gcgaactgct gcgccttggc gacggcgccg
     4261 cccttgtcga atacccggcc gccattcctg aagtcgtggt acagcacgat gacgtcgtgc
     4321 ttggtcagca cgtcgatggt ctggatatcg tcgacgtcgg tcacgaagat ccagcgatcg
     4381 aagaatttca ggtcggcctc gagcaccagt ttcaggatgt cgtgatagtt ggtggacacg
     4441 gtaatcgcga tcatgggccg tcctttctcg tggtcgatcg gggcatgccg cgcggccgca
     4501 ccttgtcgca tcgcatcatc gtccggtcga ccgggtattg cacggggatc aggccgatgt
     4561 ttccacgcca ttcccgccgc cgccccccct cgtcaaccct aggtcgttcc gtgagccggc
     4621 cgcccgtgga cgacaatcct gccagaagga tgacgcgccc catgccgctc tggttgcaga
     4681 cctacctcga cgcgctgacc aatcccgggc tgtggcgcgc gttccgtcgt gtctactggg
     4741 ccgcgttggt cggggacggc gggcgctacc tggcctgcgt cctgctggtc tggggcctgc
     4801 tgcacgtgct gctgcgcaag cgcatcgccc atcgcctgat ctccgcctgg ccgtcccgcg
     4861 ccgacctgcg gcgcgagatc acctattcct tcagctccct gctggtggtg tcggcgggca
     4921 gcgccgtgat gctggcgatg gtggtgagcg gctgggggca ggtctaccac gatccgctca
     4981 agtacggctg gacatggctg ctcctgagcc tgcccgtcgc catcgtcctg cacgacctct
     5041 acttctactg gacgcaccgg ttgctgcaca cgccctggct gttccggcac gtgcatggcg
     5101 tgcaccaccg ctcgcgccat ccgtccccat gggccgcgct ctcgttccat cccgtcgagg
     5161 cgctgatcca gggggcgccg ctgtcgctca tcctgctggt ggtgccgctc gacgactacg
     5221 tggtgttcgc cttcctgctg caccagatgc tgcgcaatgc gtacggccac gggggcgtgg
     5281 agctgttccc gcggggtttc gcgcaccact ggttctgggg acgcttcgtc accagcacgc
     5341 accatcacct gcatcacgag acggcgcagg gcaactacgg cctctggttc acctggtggg
     5401 accgctggtg cggcaccgag cgcgcggaat acctcgagcg cttcgacaag gtgacgatgc
     5461 cgcgcccgcg cccggccgac gacggcgccg gcgcggtcgc ctgacccgcc gccgcgccgc
     5521 cgcgccgctc agagcagcac gatgtcgtac tgctccgggt tcatcgtcgg ctcggcgctc
     5581 agcgagatcg gcttcctgat gaaggccgac aggtcggcca ggtgggcgct ctcctcgtcg
     5641 agcagcatct ccacgacggc cgcgctcgcg atgacgcgga actccttggg gtcgaactgg
     5701 cgcgcctcgc gcaggatctc gcgcagcacg tcgtagcaga cgctgcgcgg cgtcttgacc
     5761 tggccgcggc cggcgcaggt gggacacggt tcgcacagca tgtgggcgag cgactcgcgc
     5821 gtgcgcttgc gcgtcatctc caccaggccc aggccggtga acccgctgac ggtcaccttc
     5881 gtgcggtcct tgctcagctg cttgcggaac tcggcgagca cggcctgctg gtgctcgtcg
     5941 cgcgtcatgt cgatgaagtc gacgatgatg atgccgccca ggttgcgcag gcgcagctgg
     6001 cgcgcgatgg cgccggcggc ctcgaggttg gtcttgaaga tcgtctcgtc gaagttgcgc
     6061 gcgccgacga agccgccggt gttgacgtcg atggtcgtca gcgcctcggt ctggtcgatg
     6121 atcaggtagc cgccgctctt caagtcgacg cggcgcgcga gcgcgcgggc gatctcctcc
     6181 tcgatgttgt agaggtcgaa gatcgggcgc tcgcccttgt agtgctcgag ccgccccacc
     6241 gagctcgacg cgtacgtctc gccgaaggcg cgcagcgact cgtactgcag cttggagtcg
     6301 atgcggatcg actgcgtgtg ctcgttggcg atgtcgcgca ggacgcgctc ggccaggctc
     6361 aggtcctggt gcagcagcga gccggcgggc ttggacagcg cgcgctcgcg gatgcggttc
     6421 cacgtggtgc gcaggtaggc gatgtcctcg gccagttcct ggtcgctcgc ctcctcggcg
     6481 ttggtgcgca ggatgaagcc gccgccacct tccagcggat tgccggccag cgcgatcatg
     6541 cgggcgcgca gctgctcgcg cagctcgtgc gagccgatgc gctgcgagat gccgatgtgg
     6601 ttgtcgtgcg gcaggtagac cagcagccgg ccggcgatgc tgacctgcgt cgtgagccgc
     6661 gcgcccttgg tgccgatcgg atccttgacc acctgcaccg tgagcgactg gccttcgaag
     6721 acgagcttct cgatggccgc gggcgcgttc tcgggcttgg cggccggatg gccgtgcagg
     6781 tcggccacgt gcaggaaggc cgcgcgctcc aggccgatgt cgatgaaggc cgactgcatg
     6841 ccgggcagca cgcgggccac cttgcccagg tagacgttgc cgaccaggcc gcgttccagc
     6901 gcgcgctcca gatgcagttc ctggatggcg ccgttctcga tgatggcgac gcgcgtctct
     6961 tggggcgacc agttgaccag gatgtcgtgc atgtcggtgc cgttgtctct cgcggttctc
     7021 attcctggaa ctcgacgcct gcgtccatca gcagtcgcgc ggtctcgtgc aggggcaaac
     7081 ccatgatacc ggagtagctg cccgcgatgc gctcgatcca ggccgcgacg cggctctgga
     7141 tcgcgtaggc gccggccttg ccgaacggct cgccgctggc gacgtaggcc gcgatcgcgt
     7201 gcgcgggcat gggcgcgaag cgcaccgtcg acacgctcaa cgcgctcgtt cgcgcacggc
     7261 ccgacgccag cgccaccgcc gtcatcacgc gatgggtgcg gcccgacagg cgctccagcg
     7321 tggcgaccgc gtcgtccgcg tcggcgggct tgccgaagat gcggcgaccg agcgccacgg
     7381 tggtgtcggc gcacagcacc ggcgccggcg gcaggccgcg tttcttcatc cgcgcgacgg
     7441 cggcgtcgag cttgagctgc gtgacgcgct cgcagtagac ggccggcgtc tcgccgggga
     7501 tgtgctcctc cagcgtctcg gcgtcctcgt cgggcgatgc gagcagcagt tcgtggcgca
     7561 cgcccagctg gtcgagcagt tggcggcggc gcgggctttg cgaggcgagg tagatgaacg
     7621 ggtgtttcat catgagtgat cggtgtgtcg tccgttcgcg ggccggccgg tcgtcgaagg
     7681 ggtctggcct ctcctcgaat cgcgacgaag cgcggacgaa agccaaggat gccggtggcg
     7741 attcgaggcg aaggcctgac cccgatcgcc gctcacgttc gaggtcaggc ctgcgcctcc
     7801 gatcgcgacc cgcaaagctg ctcgcggctg cgcaacgacg cgatcgaagg agaggccaga
     7861 cccctcgggc atcggcgcgc cgtcgctcat tcgcggtgat acgggtgatt cgtcgacacc
     7921 gaccacgcgc ggtacagcgc ctcggccagc agcggccgca cgaacgcgtg cggcagcgtg
     7981 aggtcggaca gccgcgccgt ctcgtcggcg gtggccttca ggtcgggatc gaggccgtcg
     8041 gggccgccga tcaggaaggc gacgtcgcgg ccgtcgccgc gccagaactc gagccggtcg
     8101 gcgagcgcgc gcgtggtcca gcgcgtgccg cgttcgtcga gcaccacgcg ccgcacgccc
     8161 gcaggcagca cggcctccag ccgtttcgcc tcggcctgca tcatctgcgc ggcgatcttg
     8221 ccgctggtgc gcggctcggc cttgacggcc ttgagctcga gccgcatctc gggcgggaag
     8281 cggcgctcga attcctcgta cgccgcctcg gcccaggcgg gcggcttctg gccgacggag
     8341 gcgatgatca ggcgcatgtt cgtgggatgt gaaaacggcg accgaagtcg ccgtccatcg
     8401 aggtctctcg tgacgatcag ccgcggcgcg ccgcgacctt cttggcgacg gccgacttgg
     8461 cggccgccgt cttcctggcc gtcttcttgg ccgccgtcgc ggcgttgcgc gagctgttgg
     8521 ccggcacgac caccttcttg accgacgcgg ccttcaccgg cgcaccgccg gcgcgcgcgg
     8581 cggtcgagcc ggcgcgcgtc ttcttggcgg cggcgacctt gccggccgcc gacttcggcg
     8641 ccggcttggc cttgcctgcc tgcgagccgg ccggcttggt ggccgcggcc gcgcgggcgc
     8701 tggtcttggc ggcggggccg cgcgtggacg acgaacgctc ggccggcgtc ttggcgcgcg
     8761 gcagcggcgc gggcttgccc gcggccgtct tcttgccggc cggcttcttg agcgcggcct
     8821 tcctggcggg agtttcgtcg gcgtcgacga ccttggcggc cttcgcgcgc ttcctgggct
     8881 tgggcgcctc gtcggcgtcg tcgtcagcgc cttccgaggc cttgaccagt ccgccgctgc
     8941 gtccgccgcc gagcttgatc gccaccggct tgccgcccca gatctcttcc aggcggtagt
     9001 agtcgcggat cgacggctgc atgatgtgcg cgaccgccgc gccgcagtcg acgatgatcc
     9061 actcgccgtt ctcctcgccc tcggtgcgca gcacgtccat gccggcggac ttcaccgcgt
     9121 cgcgcacgct cgccgcgagc gccttggtct ggcggttgct ggcgccggcc gcgacgatca
     9181 cgcgctcgaa cagcggcgac aggtgctcgg tgttgaagac ctggatgtcc tgggccttga
     9241 cgtcttccag gccatcgacg atggcgcgtt gcagtttgcg gatatccata tcttttttta
     9301 acggggtctg gtcttgaatc gtgcgcgctg agaggcctcg cgacgctcgc acgtcgccgc
     9361 gcaagatgcg agacctgaca ccgtcatttc ggaagggtgt agaggtggtg gtcggcaata
     9421 tagcgcgcca ccggcgcgcc gaccagcgcc gtgacgtcca gcccggccgc ggcgcgctcg
     9481 cgcaccgcgg tggccgaatg gggatggggc ggcatctgga cgatgcgccg ttcgtgctcg
     9541 acgccggcca ggccgggcgc cgcgcgcacc gggtcgccgt cgcgcgcggc cacggccagt
     9601 ccggccagcg cgacgatgcc gcgccagtcg tgccaggtgt cgaaacgcgc gtactggtcc
     9661 tgcccgatga ccagccacca gcgcgtgccc ggctgctcgg cgtgcagtgc gcgcagcgtg
     9721 tcgaccgtga agctgggacc ggcgcgatcg agctcgcgcg cgtcgatcga gaagcgcggc
     9781 tcgccctcga tgagccgttg cagcatcgcg acgcgatccg cacccgaggc cggcagccgg
     9841 ccgcccttgt gcggcgactg gcgcgcgggc agccacacga gctcgtcgag cgccagttgc
     9901 tcgagcgcgc actgcgcgag ggcgcggtgc gcgaggtggg gcggatcgaa actgccgccg
     9961 aacaggcctg cgttcatgac agcccctccc gcggcgggta cgccgctgcg tcccccgagg
    10021 ggatcgcggg ccagccgggg acggcccggc gctcgtcccg cgagcgagtg gtcgtggcgg
    10081 cgctcaagcg agccagtcgc gcgggcgcag gaaatccgcg tacagcgccg cctcgggcgt
    10141 gccgtcctgc ggcgaccact ggtagcgcca ctgtgccagc ggcggcatcg acatcaggat
    10201 cgcctcggtg cgtccgcccg actgcagccc gaacagcgtg ccgcggtccc acacgaggtt
    10261 gaactcgacg tagcggccgc gccggtaggc ctggaagtcg cgctcgcgct cgccgaacgg
    10321 cagcccgcgg cggcgctcga cgatgggcag gtaggccgcg aggaacgcat cgcccaccga
    10381 gcgcagcatc gcgaagccct gctcgaagcc gagctcgttg acatcgtcga agaagatgcc
    10441 gccgatgccg cgcggctcgt tgcggtgctt caggaagaag tactcgtcgc accacttctt
    10501 gaagcgcggg tacagcgtct cgccgaacgg cgcgagcgcg tcgcggcagg tggcgtggaa
    10561 gtggcgcgcg tccgcttcgt cgccataata aggcgtgagg tccatgccgc cgccgaacca
    10621 ccagacggga tccttgccgt ccgccgtcgc ttcgaaaaag cgcacgttca tgtgcacggt
    10681 gggcacgtac gggttgcgcg ggtgcaagac gagcgagacg ccgagcgcct cgaatgcggc
    10741 gccggccagc tcgggtcgcg cggcggtggc cgacggcggc agcgtcctgc ccttgac
//
LOCUS       DATCRO010000705         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_8619, whole genome shotgun sequence.
ACCESSION   DATCRO010000705 DATCRO010000000
VERSION     DATCRO010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8619"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VIN75_19120"
     CDS             complement(<1..702)
                     /locus_tag="VIN75_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8976335.1"
                     /translation="MTYALLVVGFVLFLWYRHATKRRRKEAAHVAELQEAHAKGMTEP
                     PSLHPIIDPTRCIGSASCVRVCPEEALGIINGKAVLTNASACIGHGACLAACPVDAIK
                     LVFGTEKRGIDIPNVKPDFETNVPGIFIAGELGGMGLIRKAAEQGRQAIETIRNRKAT
                     GQAEYDVVIVGSGPAGISAGLAAIQHGLRYKLIEQEDSLGGTVYHYPRNKIAMTAPVK
                     LALVGTVRMGEIQKEA"
     gene            complement(754..2040)
                     /gene="ccmI"
                     /locus_tag="VIN75_19125"
     CDS             complement(754..2040)
                     /gene="ccmI"
                     /locus_tag="VIN75_19125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY8976336.1"
                     /translation="MTIFLLASAGLLLVALAVLTWPLLHAPGSGRALRREVDVAGRQL
                     RQLQELHAGGALTHEQFVQSRTALERRLVEALVPSDTRAAPGAPPRSRRLALVLAGFM
                     LAVTAGGYWFIGSPGHLAVGPGQFAPGMAPREASADGSQAAPHDLAPAQVQSMVDQLA
                     ARLATHPEDAQGWTMLARSRVALGQHDKAVEAFVRAERLQPDDPDLLADHADALAMAH
                     GRNLEGEPMALLQQALRIAPHHAKALALAGTAAFDRKDYRQAVQLWETLASVEPPDGP
                     FASQVRDGIAEARQLAGMPAAPASAPASAPVGTAAPLAATLSGTVTLAPALAARAAPG
                     DVLFVFARAVDGPRMPVAIVRKHVSDLPLAFTLDDGMAMAPEASLSSVRRVVVGARIS
                     HSGNAVPREGDLQGSTPVVDVGASGLHIEIDQVVAK"
     gene            2217..2888
                     /locus_tag="VIN75_19130"
     CDS             2217..2888
                     /locus_tag="VIN75_19130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976337.1"
                     /translation="MRLLLAESDQERGSDMREALNHVGYATDWVRTGTHLRGALSTQK
                     YDCAVVGLPMADVSGDALLESVRREQAPLPVILLTGTESSRDRAALLDRGADDCLMRP
                     FDFDELAARIRSLLRRVRSAEPGEDPLAHGSLLLQPQRLCAVLAGRQVKLTQCEFSVL
                     ETLVRKKHQVCSRAMLEESLYAWGDEVNSNAVEVYVHYLRRKLAPDVIVTVRGLGYQL
                     GPQPA"
     gene            complement(2918..3610)
                     /locus_tag="VIN75_19135"
     CDS             complement(2918..3610)
                     /locus_tag="VIN75_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976338.1"
                     /translation="MTTRIIIVGDNQLFRKGLSALLSTDPDLAVIGDVPTGPHVVQAV
                     TDFQPDVVLMDLQATAQNGLEAGTQIKHRHANTRIVLLMALKTEEQVRNALRAGADGC
                     LLRDASADELKACLRAVSTGRKYLSADVSALVVDSLLRPDDGHSRHSLLDALTPRERS
                     ILQLVAEGRTNRGVAEFLNVSPKTIEKHRSSLMHKLGLRNAAELTLAAVDLGLIERPT
                     SVSRLAGDGGGR"
     gene            complement(3607..4185)
                     /locus_tag="VIN75_19140"
     CDS             complement(3607..4185)
                     /locus_tag="VIN75_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8976339.1"
                     /translation="MTGLLIAAPRRRQGGAVMLAFLLALALVGIGAMAAFDVWSVTRQ
                     RQRETQLLFVGHQFRAAIRDYYYAGPPGAGRSLPPSLDVLLDDNRFPVPVHHLRRLYA
                     DPMTGSTDWGLLMAGDRVIGVYSKSEAMPIKQKGFELIDASFEDKTSYHDWVFAFVAG
                     RHAASIGVPAPASTAGIPAADSTRTPAMKKTT"
     gene            complement(4182..4577)
                     /locus_tag="VIN75_19145"
     CDS             complement(4182..4577)
                     /locus_tag="VIN75_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8976340.1"
                     /translation="MAGTERSSRTASGRTGGFTLLEMLVVMTLIALLLTLALPRYFHA
                     LDYGRANVQRQNLATIRDAIDKFHGDLGRYPDSLDELVARRYLRQVPVDPLSETSNWV
                     VVAPQDTTEGNVYDIQPPNADKAAKEPSP"
     gene            complement(4581..5084)
                     /locus_tag="VIN75_19150"
     CDS             complement(4581..5084)
                     /locus_tag="VIN75_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8976341.1"
                     /translation="MKHRQATGFTLIELLVTLALVGLLALVSLPLFEVTSTRMKEEQL
                     REALRTIRGGLDAYKRAVDTGLLPREAGASGYPPSLEVLTEPLDMANKVGAGFGSSDT
                     PQRLVILRQLPRDPFFPDPAVPAAQTWRTRAYGSRSDDPQPGDDVYDVSSMSTRVGLD
                     GIAYGAW"
BASE COUNT          717 a         1783 c         1898 g          788 t
ORIGIN      
        1 ggcctccttc tggatctcgc ccatgcgcac cgtcccgacc agcgccagct tgacgggcgc
       61 ggtcatggcg atcttgttgc gcgggtagtg gtagacggtc ccgcccagcg agtcctcctg
      121 ctcgatgagc ttgtagcgca ggccgtgctg gatggccgcc aggccggcgg agatccccgc
      181 cggcccgctg ccgacgatga ccacgtcgta ctccgcctgg cccgtcgcct tgcggttcct
      241 gatcgtctcg atggcctggc gtccctgctc ggcggccttg cggatcagtc ccatgccgcc
      301 gagctcgccc gcgatgaaga tcccgggcac gttcgtctcg aaatcgggct tgacgttcgg
      361 gatgtcgatc ccgcgcttct cggtgccgaa caccagcttg atcgcgtcca ccgggcaggc
      421 cgcgaggcac gcgccgtggc cgatgcaggc cgaggcgttc gtgagcaccg ccttgccgtt
      481 gatgatgccc agcgcctcct cggggcagac gcgcacgcac gaggccgatc cgatgcagcg
      541 cgtcgggtcg atgatcggat gcagcgacgg cggctccgtc atgcccttgg cgtgggcctc
      601 ctgcagttcg gcgacgtgcg ccgcctcctt cctgcggcgc ttggtcgcgt gccggtacca
      661 gaggaacagc acgaagccga cgacgagcag cgcataggtc atcgcggtct ccgtgcggtg
      721 cagggggcgg cttgtcgtgg cgcggccatg gggctacttc gccaccacct ggtcgatctc
      781 gatgtgcagc ccggacgcgc ccacgtcgac gaccggcgtc gagccttgca ggtcgccctc
      841 ccgcggcacg gcgttgccgc tgtgcgagat gcgcgccccg acgacgacgc gccgcacgct
      901 ggacaggctg gcctcgggcg ccatcgccat gccgtcatcg agcgtgaacg ccagcggcag
      961 gtcgctgacg tgcttgcgca cgatggccac gggcatgcgc gggccgtcga cggcccgcgc
     1021 gaagacgaac agcacgtcgc cgggcgccgc gcgggcggcc agcgccggcg cgagcgtgac
     1081 ggtgcccgac agcgtcgctg cgagcggcgc cgcggtaccc acgggtgcgg acgccggcgc
     1141 gctggccgga gcggcgggca tgccggcgag ctggcgcgcc tccgcgatgc cgtcgcgcac
     1201 ctgcgaggcg aacgggccgt ccggcggctc cacgctggcc agcgtctccc acagctgcac
     1261 ggcctgccgg tagtccttgc ggtcgaacgc cgcggtgcct gcgagcgcga gtgccttcgc
     1321 atgatgcggc gcgatccgca gcgcctgttg gagcagcgcc atcggctcgc cctcgaggtt
     1381 cctgccgtgc gccatcgcga gcgcgtcggc gtggtcggcc aggaggtcgg ggtcgtcggg
     1441 ctgcaggcgt tcggcgcgca cgaacgcttc gacggccttg tcgtgctggc cgagcgccac
     1501 gcgcgagcgg gcgagcatcg tccagccctg ggcgtcctcc ggatgcgtcg ccagccgcgc
     1561 cgccagctgg tccaccatcg actggacctg cgccggtgcc aggtcgtgcg gcgcagcctg
     1621 cgatccgtcg gcgctcgcct cgcgtggcgc catgcccggc gcgaactggc cggggccgac
     1681 cgccagatga cccggcgacc cgatgaacca gtagccgccg gcggtcaccg ccagcatgaa
     1741 ccccgccagc accagcgcca gtcggcgcga acggggcggg gcgccggggg cggcgcgggt
     1801 gtcggacggc accagggcct cgaccaggcg ccgctccagc gcggtgcggc tctggacgaa
     1861 ctgttcatgg gtgagggcgc ctccggcgtg cagttcctgc agctggcgca gttgccgtcc
     1921 ggcgacatcg acctcgcggc gcagcgcccg cccgctgccc ggggcatgca gcagcggcca
     1981 cgtgaggacc gccagggcca ccagcagcag gcctgccgac gcgagaagga agatcgtcat
     2041 gccgggtctc cgcagacacg ggcctcgtgc cgtccgccgg cgccggcgcc gatagcttgc
     2101 gccacgctta agtggcgtcg ccataagccg aacaggcacc agtgcttatt tacacgcgca
     2161 aggcgcttgt atagatttga aacagtaaaa gcgcgtgccg aggaggggtg aaggccatgc
     2221 gacttctttt ggcagagagt gatcaggaac gcggcagcga catgcgcgag gcgctgaatc
     2281 acgtcggcta tgcgaccgac tgggtgcgca cgggcacgca cctgcgcggc gccctgtcga
     2341 cgcagaagta cgactgcgcg gtcgtcgggc ttccgatggc cgacgtgtcg ggcgacgccc
     2401 tgctggagtc ggtgcgccgc gaacaggcgc cgctgccggt catcctgctc accggcacgg
     2461 agtcgtcgcg cgaccgggcg gcgctcctgg accgcggcgc ggacgactgc ctgatgcgtc
     2521 cgttcgactt cgacgaactg gccgcgcgca tccgttcgct gctgcggcgc gtccgatccg
     2581 cggaaccggg ggaagatccg ttggcacatg ggtcgctcct gctgcagccg cagcggctct
     2641 gcgcggtgct cgccggccgc caggtgaagc tcacgcaatg cgaattctcc gtactggaga
     2701 cgctcgtgcg caagaagcac caggtctgca gccgcgcgat gctcgaggag tctttgtacg
     2761 cctggggcga cgaggtgaac agcaacgcgg tggaagtcta cgtccactac ctgcgccgca
     2821 agctcgcgcc tgacgtcatc gtgaccgtgc gcgggctcgg ctaccagctc gggcctcagc
     2881 ctgcctgaag cgtgcgcgcg ccgcgccggc gcgcccctca ccggccgccc ccgtcacctg
     2941 ccagccggct cacggatgtc ggccgttcga tcaggccgag gtcgaccgcc gcgagcgtga
     3001 gctccgcggc gttgcgcagg cccagcttgt gcatcaggct ggaacggtgc ttctcgatcg
     3061 tcttcgggct gacgttgagg aattccgcca cgccgcgatt ggtgcgtccc tcggccacca
     3121 gttgcaggat gctgcgctcg cgcggcgtca gcgcgtccag cagcgaatgg cgcgagtggc
     3181 catcgtcggg tcgcaacagg ctgtcgacga cgagcgccga gacgtcggcg ctgagatatt
     3241 tgcgtcccgt cgacaccgcg cgcaggcagg ccttgagctc gtcggccgac gcatcgcgca
     3301 gcaggcagcc gtcggcgccg gcgcgcaggg cgttgcgcac ctgctcctcg gtcttgagcg
     3361 ccatcagcag gacgatgcgc gtgttcgcat gccgatgctt gatctgggtg cctgcctcga
     3421 gtccgttctg ggcggtggcc tgcaggtcca tcaggacgac atccggctgg aagtcggtca
     3481 cggcctgcac gacgtgcgga ccggtgggga cgtcgcctat gacggcgagg tcgggatccg
     3541 tggagagcag tgccgacagg cccttgcgga acaactggtt gtcgccgacg atgatgatgc
     3601 gtgtagtcat gttgtcttct tcatggctgg cgtgcgggtg gaatcggccg ccgggatccc
     3661 tgcggtactg gcgggtgcgg gcaccccgat cgaggcggca tggcggccgg cgacgaatgc
     3721 gaatacccag tcgtggtacg acgtcttgtc ctcgaacgag gcgtcgatca gctcgaatcc
     3781 cttctgcttg atgggcatcg cttcggactt gctgtagacg ccgatgacgc ggtcacccgc
     3841 catcagcagg ccccagtcgg tcgagccggt catcggatcc gcatagagcc ggcgcaagtg
     3901 gtgcaccggc acgggaaagc ggttgtcgtc cagcagcacg tcgaggctgg gcggcaggct
     3961 gcggcctgcg ccgggcgggc cggcatagta gtagtcgcgg atcgcggcgc ggaactggtg
     4021 cccgacgaac agcagctgtg tctcgcgttg gcgctgccgc gtgacgctcc acacgtcgaa
     4081 cgcggccatc gcgccgatgc ccacgagcgc cagcgccagc aggaaggcga gcatcacggc
     4141 gccgccctgc cgccgccgcg gcgccgcgat gaggagtccg gtcatggcga cggctccttg
     4201 gcggccttgt ccgcgttggg tggctggatg tcgtagacgt tgccctccgt cgtgtcctgc
     4261 ggcgcgacga ccacccagtt cgaggtctcg gacagcggat ccaccggcac ctgccgcagg
     4321 tagcggcgtg ccacgagttc gtccagcgag tcgggatagc gcccgaggtc gccgtggaac
     4381 ttgtcgatgg cgtcgcggat cgtggccagg ttctgccgct gcacgttggc gcggccgtag
     4441 tcgagcgcgt ggaagtagcg gggcagggcg agcgtgagca gcagcgcgat gagcgtcatc
     4501 accaccagca tctccagcag cgtgaacccg cccgtccggc cggaggccgt gcgcgaggag
     4561 cgttccgtgc cggccatggt tcaccacgcg ccgtaggcga tgccgtcgag gccgacgcgg
     4621 gtcgacatcg acgagacgtc gtagacgtcg tcgccgggct gcggatcgtc gctgcggctg
     4681 ccgtaggcgc gcgtgcgcca ggtctgcgcc gcgggcacgg ccgggtcggg gaagaacgga
     4741 tcgcgcggca gctggcgcag gatgaccagg cgttgcggcg tgtccgacga tccgaagccg
     4801 gcgccgacct tgttggccat gtccagcggc tcggtgagga cctcgagcga cggcgggtag
     4861 ccggacgcgc cggcctcgcg cggcagcagg cccgtgtcga cggccctctt gtacgcatcg
     4921 aggccgccgc ggatggttcg cagcgcctcg cgcagctgtt cctccttcat gcgcgtagac
     4981 gtgacctcga acagcggcag gctcaccagc gcgagcaggc ccacgagagc gagcgtcacg
     5041 agcagctcga tcagcgtgaa gccggtggct tggcgatgtt tcattgcccg gcctccccgg
     5101 ggtccgacgc cggcgcgggg gcgggcatgg cggccgctgg cgcgggggcc gcggcgctcg
     5161 cgggcgcgcc acggtgcccg ttgagc
//
LOCUS       DATCRO010000706         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1043130, whole genome shotgun sequence.
ACCESSION   DATCRO010000706 DATCRO010000000
VERSION     DATCRO010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043130"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIN75_19155"
     CDS             complement(<1..219)
                     /locus_tag="VIN75_19155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8976342.1"
                     /translation="MELVERDAALLTFEHQLAAARQGGGHLVLVSGEAGIGKTTLLKA
                     MAARRGDAALWWGACDPLETPHPLAPLHD"
     gene            complement(224..1060)
                     /locus_tag="VIN75_19160"
     CDS             complement(224..1060)
                     /locus_tag="VIN75_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY8976343.1"
                     /translation="MSDTAGALDLDGYLRRIGFAGTPAPTRGCLDELVARHASAVPFE
                     NIEVLARRVPRLDLDGLQAKMLRRRRGGYCFEQNTLMRSALQAIGFDAHAMEARIRSN
                     VPADVVTARTHLATRVVLDGVDLLVDVGCGVLAPTAPLALARRDTQAAGSGLYRLVDA
                     GDELLLQQREPAGWKDCYQLQPRAPHPIDCEMGNWFVATWPKSMLAANLLVGRSIEGG
                     RLRLFNRRLSAFQPEHAAPVERELQTRAEFADVLADGFGLDVAPADLDAVMAALDRLP
                     PA"
     gene            complement(1057..2370)
                     /locus_tag="VIN75_19165"
     CDS             complement(1057..2370)
                     /locus_tag="VIN75_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8976344.1"
                     /translation="MSLPRRDFLSLLATAPVLAMTSSHAATTPDMPHVPWSRQAVIYQ
                     VNVRQYSADGTLKSAMAELPRLKAMGVDILWLMPIQPIGQVNRKGTLGSYYSIRDYTA
                     VNPEFGALDDARAFVQAAHAQGMKVILDWVANHTAFDHPWTREHKDWYRLDAHGQVFP
                     VTFNAGTPGEEHWADVTQLNYDAPALRDAMIAAMMFWVREVDLDGFRCDVAGEVPTPF
                     WERARRELDAVKPMFMLAEADKIELHRAAFDMTYSWDLAQLFERIGQGKAGAAELRRW
                     LAREPHGYPASAYRMRFTSNHDFNSWNGTDAQLYGDNYLPLAMLTFTLPGMPLIYGGQ
                     EARLAKRLEFFEKDPIDWKTRELQPFYTSMAALKHRHPALAAGQYGGASTVLDVRNDR
                     LFAFERRKEADRVTVIVNVTSAEQKFERNGAEHALAPHTWLIEAA"
     gene            complement(2431..3369)
                     /locus_tag="VIN75_19170"
     CDS             complement(2431..3369)
                     /locus_tag="VIN75_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976345.1"
                     /translation="MREINQQRLRYFREVLAHGSIRGAADALNTAPSVVTRQIRLLEH
                     ELDTTLFERQARGVRPTDAATHLLEFWRGYRTHQEHLEDRLQALRGLRSGSVRLAISE
                     GFVDVLVDQVLAAFCARHPGLEIAIDTLSADALVREVAESRAHIGLAYNPPADAAIER
                     LASSRQPIVAMARPDHPLARRKAPARIADLLAHPLALMPGSFGIGQAVDALSRAENVA
                     LRPALTTNSLAALKRVAATGSFVALVGEFAAHREVAAGELAVVRIDHALFRGIHARVL
                     VRAGRPLATAPRELLAWILREMPLFASTRRRRTAPR"
     gene            3478..4530
                     /locus_tag="VIN75_19175"
     CDS             3478..4530
                     /locus_tag="VIN75_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976346.1"
                     /translation="MSTVSAPSRRLFLGTAALGLTAATAQLALASTPHAQSAPASVGA
                     QRALASFGPLRQIDAGLLNVGFVDVGPSDGRVVILLHGWPYDIHSFADVAPRLVAAGF
                     RVIVPHLRGHGSTRFLSPATLRNGQQAAVALDIVALMDALDIRKATFGAFDWGARTAD
                     IIAAMFPERCEGLVSVSGYLINNREAVKAPLPPAAEYAWWYQFYFATEHGKAGYARNR
                     HDFNKLIWKTASPRWDFSDAEFERSAASFDNPDHVDVVIHNYRWRQSLAEGEARYDDL
                     ERQLAAGPAICVPSIVMEGDANGAPHFTGDAAFRRKFTGPYAYRVVAGGIGHNLPQEA
                     PREFAQAVIDVAALRL"
BASE COUNT          621 a         1630 c         1660 g          660 t
ORIGIN      
        1 gtcgtgcagc ggcgcgagcg ggtgcggcgt ctcgaggggg tcgcaggcgc cccaccacag
       61 cgcggcatcg ccgcggcgcg cggccatcgc cttgagcagc gtcgtcttgc cgatgcccgc
      121 ctcgccgctc accagcacca ggtggccgcc gccctggcgt gccgccgcga gctggtgctc
      181 gaacgtcagc agggcggcgt cgcgctcgac cagctccatg gcgtcatgcc ggcggcaggc
      241 ggtcgagcgc cgccatgacg gcgtccaggt cggcgggcgc cacgtccagg ccgaagccgt
      301 cggccagcac gtcggcgaac tccgcgcgcg tctgcagctc gcgttcgacc ggcgcggcgt
      361 gctcgggctg gaacgccgac agccgccggt tgaacaggcg caggcggccg ccctcgatcg
      421 agcggcccac cagcaggttg gcggccagca tcgacttggg ccaggtggcc acgaaccagt
      481 tgcccatctc gcagtcgatc gggtgcggcg cacgcggctg cagctggtag cagtccttcc
      541 agcccgcggg ctcgcgttgc tgcagcagca gctcgtcgcc ggcgtccacc aggcgataca
      601 ggcccgagcc ggcggcctgc gtgtcgcgcc tcgcgagggc cagcggcgcg gtcggcgcca
      661 gcacgccgca gccgacgtcg acgagcaggt cgacgccgtc gagcacgacc cgcgtggcca
      721 ggtgcgtacg cgccgtgacg acgtcggccg gcacgttcga gcggatgcgc gcctccatcg
      781 cgtgcgcgtc gaagccgatg gcctgcagcg cgctgcgcat cagcgtgttc tgctcgaagc
      841 agtagccgcc gcggcgccgg cgcagcatct tggcctgcag gccgtcgagg tccaggcgcg
      901 gcacgcggcg cgccagcacc tcgatgttct cgaacggcac ggcgctcgcg tgccgggcca
      961 ccagctcgtc caggcatccg cgcgtgggcg ccggcgtgcc tgcgaagccg atgcggcgca
     1021 ggtagccgtc caggtccagt gcgccggccg tgtcgctcat gcggcctcga tcagccaggt
     1081 gtgcggcgcg agcgcgtgct cggcgccgtt gcgctcgaac ttctgctcgg cggacgtgac
     1141 gttgacgatg accgtgacgc ggtccgcctc cttgcggcgc tcgaacgcga acagccggtc
     1201 gttgcggacg tccagcaccg tcgacgcgcc gccgtactgg ccggccgcga gcgcgggatg
     1261 ccggtgcttg agcgcggcca tcgacgtgta gaagggctgc agctcgcgcg tcttccagtc
     1321 gatcgggtcc ttctcgaaga attccaggcg cttggcgagg cgcgcctcct ggccgccgta
     1381 gatcagcggc atgcccggca gcgtgaacgt gagcatggcc aggggcaggt agttgtcgcc
     1441 gtacagttgc gcgtcggtgc cgttccacga gttgaagtcg tggttgctgg tgaagcgcat
     1501 gcggtaggcc gacgccgggt agccgtgcgg ctccctggcg agccaccggc gcagctcggc
     1561 cgccccggcc ttgccctggc cgatgcgctc gaacagctgc gcgaggtccc agctgtaggt
     1621 catgtcgaag gccgcgcggt gcagctcgat cttgtcggcc tcggccagca tgaacatcgg
     1681 cttgaccgca tcgagctcgc gccgcgcgcg ctcccagaac ggcgtgggca cctcgccggc
     1741 gacgtcgcag cggaagccgt cgaggtcgac ctcgcgcacc cagaacatca tggccgcgat
     1801 catcgcgtcg cgcagcgccg gcgcgtcata gttcagctgc gtgacgtcgg cccagtgctc
     1861 ctcgccgggc gtgccggcgt tgaacgtgac gggaaagacc tggccgtgcg cgtcgagcct
     1921 gtaccagtcc ttgtgctcgc gagtccaagg gtggtcgaag gccgtatggt tggcgaccca
     1981 gtcgaggatc accttcatgc cctgagcgtg cgcggcctgc acgaaggcgc gcgcgtcgtc
     2041 cagcgcgccg aactccggat tgaccgcggt gtagtcgcgg atcgagtagt agctgccgag
     2101 cgtgcccttg cggttgacct ggccgatcgg ctggatcggc atcagccaca ggatgtccac
     2161 gcccatcgcc ttgagccgcg gcagttcggc catcgcggac ttgagcgtgc cgtccgcgct
     2221 gtactggcgc acgttgacct ggtagatcac ggcctggcgc gaccacggca cgtgaggcat
     2281 gtcgggtgtc gtggcggcgt gggacgaggt catggcgagg acgggcgcgg tggcgagcag
     2341 cgacaggaag tcgcggcggg gcagggacat ggcgtgcctc cttggccggg ccagtttagt
     2401 cccgtggccg gccgggcgcg atcgtcgtgc tcagcgaggc gccgtgcgcc gccggcgggt
     2461 gctcgcgaac agcggcatct cgcgcaggat ccaggcgagc agttcgcgcg gggccgtcgc
     2521 cagcggccgg ccggcgcgca ccagcacgcg cgcatggatg ccgcggaaca gcgcgtggtc
     2581 gatccgaacg accgccagct cgccggcggc cacctcgcgg tgcgcggcga actcgcccac
     2641 cagcgcgacg aagctgccgg tggccgcgac gcgcttgagc gcggccagcg agttggtggt
     2701 cagcgcgggc cgcagcgcga cgttctcggc ccgcgacaac gcatcgacgg cctgcccgat
     2761 gccgaacgag ccgggcatca gcgccagcgg atgcgcgagc aggtcggcga tgcgggccgg
     2821 cgccttgcgc ctggcgagcg ggtgatcggg ccgcgccatc gccacgatcg gctggcgcga
     2881 gctcgcgagg cgctcgatcg ccgcgtcggc cggcgggttg taggcgagac cgatgtgcgc
     2941 ccggctctcg gccacctcgc gcacgagcgc gtccgccgag agcgtgtcga tggcgatctc
     3001 cagccccggg tggcgcgcgc agaaggcggc cagcacctgg tcgaccagca cgtcgacgaa
     3061 gccctcgctg atggccagcc ggacgctgcc cgagcgcagc ccgcgcagcg cctgcaggcg
     3121 gtcctcgagg tgctcctggt gcgtgcggta gccgcgccag aactccagca ggtgcgtggc
     3181 cgcatcggtc ggcctgacgc cgcgcgcctg gcgttcgaac agcgtcgtgt cgagctcgtg
     3241 ctcgagcagc ctgatctggc gcgtgacgac cgacggcgcc gtgttgagcg cgtcggcggc
     3301 gccgcggatg ctgccgtgcg cgagcacctc gcggaagtag cgcaggcgtt gctggttgat
     3361 ctcgcgcatg gggcctcccg gaacgttgct ccgatggcaa cgatccgcga tcttagttgc
     3421 tcttgttcgg tgcaccggga acatcgaaca tgcggccatc gcagcaagga gtccgccatg
     3481 tccaccgttt ccgccccgtc gcgccgcctg ttcctcggca ccgccgccct cggcctgacc
     3541 gccgccaccg cgcagctcgc gctggcgtcg acgccgcatg cgcaatcggc gcccgcgtcc
     3601 gtcggcgcgc aacgcgcgct cgcgagcttc gggccgttgc gccagatcga cgccgggctg
     3661 ctgaacgtcg gcttcgtcga cgtcggcccg tccgacggcc gcgtcgtcat cctgctgcac
     3721 ggctggccct acgacatcca cagcttcgcc gacgttgccc cgcgcctggt ggccgcgggc
     3781 tttcgcgtca tcgtgccgca cctgcgcggg cacggcagca cgcgcttcct gtctcccgcg
     3841 acgctgcgca acgggcagca ggcagcggtg gcgctggaca tcgtcgcttt gatggacgcg
     3901 ctggacatcc gcaaggccac cttcggcgcg ttcgactggg gcgcgcgcac cgccgacatc
     3961 atcgcggcga tgttccccga gcgctgcgag gggctggtct cggtcagcgg ctacctgatc
     4021 aacaaccgcg aggccgtcaa ggcgccgctg ccgcccgcgg ccgaatatgc ctggtggtac
     4081 cagttctact tcgccaccga gcacggcaag gcgggctacg cgaggaaccg gcacgacttc
     4141 aacaagctga tctggaagac ggcctcgccc aggtgggact tcagcgacgc cgagttcgag
     4201 cgcagcgccg cctcgttcga caaccccgac cacgtcgacg tcgtgatcca caactaccgc
     4261 tggcgccaga gcctggccga gggcgaggcc cgctacgacg acctcgagcg acaactcgcc
     4321 gccggaccgg cgatctgcgt gccgagcatc gtgatggagg gcgacgccaa cggcgcgccg
     4381 cacttcaccg gcgacgcggc gttccgcagg aagttcaccg gaccctacgc ctaccgcgtg
     4441 gtcgcgggcg gcatcgggca caacctgccg caggaggcgc cgcgcgagtt cgcgcaggcg
     4501 gtgatcgacg tggccgctct gcgcctttga tcgcgcgatg cgcgtctgca ggcggacaat
     4561 cgtccccggg c
//
LOCUS       DATCRO010000707         7370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_615610, whole genome shotgun sequence.
ACCESSION   DATCRO010000707 DATCRO010000000
VERSION     DATCRO010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615610"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1114
                     /locus_tag="VIN75_19180"
     CDS             23..1114
                     /locus_tag="VIN75_19180"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8976347.1"
                     /translation="MSLSERTSDTDDQRIRDVTPLPPPEHLIRFFPIAGTPVETLIGD
                     TRNAVRNIVAGQDDRLLVVIGPCSIHDPAAALEYARKLKAERDRYSDTLEIVMRVYFE
                     KPRTTVGWKGLINDPYLDESYRIHEGLRIARQLLLDINRLGVPAAGEFLDVISPQYIG
                     DLISWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNLKIAIDAIQAAGRPHHFMSV
                     HKNGQVAIVETSGNKDCHVILRGGKAPNYDAASVKAACAELEAAKLACRLMIDFSHAN
                     SEKKYERQVAVARDVADQLASGNECIFGVMVESHLNAGAQKFTPGKDDPAKLEYGKSI
                     TDGCIGWDDSIAVLDVLRDAVLARRGAKA"
     gene            complement(1197..2987)
                     /locus_tag="VIN75_19185"
     CDS             complement(1197..2987)
                     /locus_tag="VIN75_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY8976348.1"
                     /translation="MAKTVGDFIVERLHQWGVRLMFGYPGDGINGVFGALNRAQGKIR
                     FIQVRHEEMAAFMASAYAKFTGELGVCIATSGPGAAHLVTGLYDARLDHMPVLAITGQ
                     QARDALGGHYQQEVDLASLFKDVAGSYVQQAGMPAQVRHIVDRAVRVAVAERRVTAMI
                     LPNDLQEESYEDPPRAHGTLHSGVGFARPVVVPAPADLQRAADVLNAGRKVALLVGAG
                     ALQATDEVIAVADALQAGAAKALLGKAALPDDLDWVTGSIGLLGTEPSWKMMNECDTL
                     LMIGSGFPYAEFLPKEGQARAVQIDIAPDMLSLRYPMEVNLVGDAALTLRALLPLLRP
                     KTDNAWRDDIAGWMTDWWKKLEDRALQPATPVNPQRVAWELSPRLPSDVILTSDSGSC
                     ANWYARDLRMRRGMMASLSGGLASMGAAVPYAIAAKFAHPGRPVVALVGDGAMQMNNM
                     AELITVSKYWQQWSDPRWIVCVFNNQDLNQVTWEQRVMEGDPKFDASQQIPDVPYHKF
                     AELIGLRGLHVDDPAQLGGAWDEALASDRPVVLEVKTDPEVPPLPPHITLKQATAFAK
                     ALAKGDPREGGFFIGAMRQVLGTILPGDHR"
     gene            complement(3166..3753)
                     /locus_tag="VIN75_19190"
     CDS             complement(3166..3753)
                     /locus_tag="VIN75_19190"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8976349.1"
                     /translation="MANRLSQIATRTGDDGTTGLGDGTRVAKSHGRIGAMGDVDELNS
                     QLGVLLAEPLPDDVRELVVTIQHELFNLGGELSIPGFTLLKDDAVAGLDAALARYNET
                     LPRLMEFILPAGTRSAALAHVARAVARRAERAVVTLAETEAINAAPRLFLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWKSEKLSRNLDPNVP"
     gene            3845..5263
                     /locus_tag="VIN75_19195"
     CDS             3845..5263
                     /locus_tag="VIN75_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8976350.1"
                     /translation="MNTAADLPFQVQPRPMPAAMAEALRDRFAERFVTSLAVREQHGR
                     DESPFEVPPPEAVVFCESTDDVAFVVAAAARHRVPVIPYGAGSSLEGHLLAVQGGVSV
                     DVSRMSRVLRLNPEDLTVTVQAGVTRMQLNREIKDSGLFFPIDPGADATIGGMSATRA
                     SGTNAVRYGTMRENVLALTVVTASGEVIRTGTRARKSSAGYDLTRLMVGSEGTLGVIT
                     EITLKLHPLPESVAAAICSFDDATAAVDTVIAIIQMGVPIARCELLDANAVIAVNRND
                     KLGLAEKPMLLMEFHGSETSVAEQAATVQELAAERGGTAFQWATTPEERTRLWTARHH
                     AFLSGLQLRPGCRAVTTDCCVPISGLADAVTQALAEAEAAGIPHFLVGHVGDGNFHVA
                     YLIDPASAADRETAERLNHQLVQRAIAAGGTCTGEHGIGLHKQGFLLEETGAGAVAMM
                     REIKRALDPHDIMNPGKIFGPA"
     gene            complement(5327..5734)
                     /locus_tag="VIN75_19200"
     CDS             complement(5327..5734)
                     /locus_tag="VIN75_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976351.1"
                     /translation="MLKIAMLAAAVALAASAGTTTNPAADPVTSSAAAADTPCHPGQP
                     LELACALRVADAAGDASDAPAELASLAPPVASSFDGSPHRSADDLAFKDAATDAGSVL
                     PAGLDRERDHSHRLAPALLALGALVILLRRRPT"
     gene            complement(5871..>7370)
                     /locus_tag="VIN75_19205"
     CDS             complement(5871..>7370)
                     /locus_tag="VIN75_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY8976352.1"
                     /translation="AQPLPSPSDWTFDMIERYHEVIRETAQRFGLDTYPNQLEIITSE
                     QMMDAYASVGMPVNYRHWSYGKEFIATEKNYKRGQMGLAYEIVINSDPCISYLMEENT
                     MAMQALVIAHAAYGHNSFFKGNYLFRMWTDASSIIDYLVYSKDYVARCEERHGIDAVE
                     ELLDSCHALMNHGVDRYRRPSKLSLAQEQARREDREAYAQLQVNDLWRTLPRRAASDD
                     SASEERRFPAEPQENLLYFIEKNAPLLEPWQREIVRIVRKIAQYFYPQRQTQVMNEGW
                     ATFWHHRLLNQMYDDGYLSDGMMIEWLKSHTNVVAQPPVTSRHYSGINPYALGFAMYT
                     DIKRICECPTDEDRQWFPDFAGSPWLPTLDHAMRNFKDESFIGQYLSPKLMRDFRFFA
                     IVDDEKDSELEISAIHDENGYRALRESLSRQYDLGSREPNIQVWNVNLRGDRSLTLRH
                     SQHNDRPLHESSQEVLKHVARLWGFDVHLESVNGKGETTRRWSVTPPHG"
BASE COUNT         1096 a         2563 c         2607 g         1104 t
ORIGIN      
        1 gacgcccgaa gcgcgccaca cgctgtcgct gagcgagcgc accagcgaca ccgacgacca
       61 gcgcatccgc gacgtcaccc cgctgccccc gcccgagcac ctgatccgct tcttcccgat
      121 cgcgggcacg ccggtggaga cgctcatcgg cgatacgcgc aacgccgtgc gcaacatcgt
      181 cgccggccag gacgaccgtc tgctggtggt gatcggcccg tgctccatcc acgacccggc
      241 cgcggcgctc gagtacgcgc gcaagctcaa ggccgagcgc gacaggtact cggacacgct
      301 cgagatcgtg atgcgcgtgt acttcgagaa gccgcgcacc accgtcggct ggaagggcct
      361 gatcaacgac ccgtacctgg acgagagcta ccgcatccac gagggcctgc gcatcgcgcg
      421 ccagctgctg ctggacatca accgcctggg cgtgcctgcc gcgggcgagt tcctggacgt
      481 gatctcgccg cagtacatcg gcgacctgat cagctggggc gcgatcggcg cccgcaccac
      541 cgagagccag gtgcaccgcg agctcgcctc ggggttgtcg gcgccggtgg gcttcaagaa
      601 cggcaccgac ggcaacctca agatcgccat cgacgcgatc caggccgcgg gccggccgca
      661 ccacttcatg tcggtgcaca agaacggcca ggtcgccatc gtcgagacca gcggcaacaa
      721 ggactgccac gtgatcctgc gcggcggcaa ggcgcccaac tacgacgcgg ccagcgtcaa
      781 ggccgcgtgc gcggagctcg aggccgcgaa gctcgcgtgc cggctgatga tcgacttcag
      841 ccacgccaac agcgagaaga agtacgagcg ccaggtggcc gtcgcgcgcg acgtggccga
      901 ccagctcgcc tcgggcaacg agtgcatctt cggcgtgatg gtggagagcc acctgaacgc
      961 cggggcgcag aagttcacgc cgggcaagga cgatccggcc aagctcgagt acggcaagag
     1021 catcaccgac ggctgcatcg gctgggacga ctccatcgcg gtgctggacg tgctgcgcga
     1081 cgcggtgctc gcgcggcgcg gcgccaaggc ctgacgctcg gcgccgttcg cggacgcgtc
     1141 acgcgcgcgc gaacgcgcct gtcgtccaag cgcgttccgg ctcgccggcc gcgggttcat
     1201 cgatggtcgc ccggcaggat ggtgcccagt acctggcgca tcgcgccgat gaagaagccg
     1261 ccctcgcgcg ggtcgccctt ggccagcgcc ttggcgaagg cggtcgcctg cttgagcgtg
     1321 atgtgcggcg gcagcggcgg cacctcgggg tcggtcttga cctccagcac gaccggccgg
     1381 tcgctcgcga gcgcctcgtc ccaggcgccc ccgagctgcg ccggatcgtc gacatggagg
     1441 ccgcgcaggc cgatcagctc ggcgaacttg tgataaggca cgtccgggat ctgctgcgac
     1501 gcgtcgaact tcggatcgcc ctccatcacg cgctgctccc acgtgacctg gttcaggtcc
     1561 tggttgttga agacgcacac gatccagcgc gggtcgctcc actgctgcca gtacttggag
     1621 accgtgatga gctcggccat gttgttcatc tgcatcgcgc cgtcgcccac cagcgcgacg
     1681 acgggccggc ccggatgcgc gaacttggcg gcgatcgcgt agggcacggc ggcgcccatc
     1741 gaggcgaggc cgcccgacag cgacgccatc atcccgcgcc gcatgcgcag gtcgcgcgcg
     1801 taccagttgg cgcacgaacc cgagtcgctc gtcagaatca cgtcgctcgg cagccgcggc
     1861 gacagctccc aggccacgcg ctgcgggttg accggcgtgg ccggctgcag cgcgcgatcc
     1921 tcgagcttct tccaccagtc cgtcatccag ccggcgatgt cgtcgcgcca ggcgttgtcg
     1981 gtcttcgggc gcagcagcgg cagcagcgcg cgaagcgtca gcgccgcatc gcccacgagg
     2041 ttgacctcca tcggatagcg cagcgacagc atgtccggcg cgatgtcgat ctgcaccgcc
     2101 cgcgcctggc cttccttcgg caggaactcc gcgtacggga agcccgagcc gatcatcagc
     2161 agggtgtcgc actcgttcat catcttccag ctgggttcgg tgcccagcag gccgatcgag
     2221 cccgtgaccc agtccaggtc gtcgggcagg gccgccttgc ccagcagcgc cttggcggcg
     2281 ccggcctgca gcgcatcggc cacggcgatg acctcgtcgg tcgcctgcag cgcgcccgcg
     2341 cccaccagca gcgcgacctt cctgcccgcg ttgagcacgt cggcggcgcg ctgcaggtcc
     2401 gctggcgccg gcacgacgac cggccgggcg aagcccacgc ccgagtgcag cgtgccgtgc
     2461 gcccgcggcg ggtcctcgta ggactcttcc tgcagatcgt tcggcaggat catcgcggtg
     2521 acgcgtcgct cggcgacggc cacgcggacc gcgcggtcga cgatgtggcg cacctgggcc
     2581 ggcatgccgg cctgctggac atacgagccg gccacgtcct tgaacagcga cgccaggtcg
     2641 acctcctgct gataatggcc gccgagcgcg tcgcgcgcct gctggccggt gatcgccagc
     2701 acgggcatgt ggtcgagccg ggcgtcgtac agcccggtga ccaggtgcgc ggcgccgggg
     2761 ccggaggtcg cgatgcagac cccgagctcg ccggtgaact tggcgtaggc cgaggccatg
     2821 aatgccgcca tctcctcatg gcgcacctgg atgaagcgga tcttcccttg ggcccggttc
     2881 agggcgccga agacgccgtt gatgccgtcg cccgggtagc cgaacatcag gcggacgccc
     2941 cattggtgga gccgctcgac gatgaagtcg cccacagtct tggccatgcc tgaatcccgc
     3001 tcgatgccac cagacgtcct ggcgggccgt tcgagcaatc gatggaccgc ggcagcatgg
     3061 gcggcgtcgc ccgctatcca gccgtagcgc gaccggggcg agaaggccgc ccggagaagc
     3121 tcgagggctg aacgcgagcg aggatgtcct ggccggcggc aaacttcagg gcacgttcgg
     3181 atccaggttg cgcgacagct tctcgctctt ccagtagacg tcatcgccgc ccagcccgtc
     3241 gaggttggcg cggttgagca cgcgggccag cacgaacagc aggtcggaca ggcggttcag
     3301 gaacaggcgc ggcgcggcgt tgatcgcctc ggtctcggca agtgtgacga cggcgcgttc
     3361 ggcgcgccgc gcgaccgcgc gcgcgacatg cgcgagcgcc gcactgcgcg tgcccgcggg
     3421 caggatgaac tccatcaggc gcggcagcgt ctcgttgtag cgcgccagtg cggcgtccag
     3481 gcccgcgacc gcgtcgtcct tgagcagcgt gaagcccgga atggacagct cgccgccgag
     3541 gttgaacagc tcgtgctgga tcgtcacgac cagctcgcgc acgtcgtcgg gcagcggctc
     3601 ggccagcagc acgcccagct gcgagttgag ctcgtccacg tcgcccatgg cgccgatgcg
     3661 cccgtggctc ttggccacgc gcgtgccgtc gcccagtccg gtggtgccgt cgtcgccggt
     3721 gcgcgtggcg atctgtgaga gtcgattggc catggcgcag tttagatcgc gcgcggcgcc
     3781 ttgacgggcg ccggcgtaga ctggccggag cggccgccaa cgaacgcccg cgcccggagt
     3841 gcccatgaac accgccgccg accttccctt ccaggtgcag ccgcgcccga tgccggccgc
     3901 gatggccgag gccctgcgcg accgcttcgc cgagcgtttc gtcacctcgc tggccgtgcg
     3961 cgagcagcac ggccgcgacg aatccccgtt cgaggtgccg ccgcccgagg ccgtggtgtt
     4021 ctgcgagagc accgacgatg tcgcgttcgt cgtcgcggcc gcggcccggc atcgcgtgcc
     4081 ggtgatcccg tacggcgccg gttcgtcgct ggagggccat ctgctggccg tgcagggcgg
     4141 cgtcagcgtc gacgtctcgc gcatgagccg cgtgcttcga ttgaacccgg aagacctgac
     4201 cgtgaccgtg caggccggcg tcacgcggat gcagctcaat cgcgagatca aggactcggg
     4261 cctgttcttc ccgatcgacc cgggcgccga cgcgacgatc ggcggcatgt cggccacgcg
     4321 cgcatcgggc acgaacgccg tgcgctacgg cacgatgcgc gagaacgtgc tggcgctgac
     4381 ggtggtcacg gcgtcgggcg aggtcatccg caccggcacg cgggcgcgca agtccagcgc
     4441 cggctacgac ctgacccgcc tgatggtagg cagcgagggc acgctgggcg tcatcaccga
     4501 gatcacgctg aagctgcacc cgctgcccga gagcgtggcc gcggccatct gctcgttcga
     4561 cgacgcgacc gccgcggtcg acaccgtgat cgccatcatc cagatggggg ttcccatcgc
     4621 gcgctgcgag ctgctggacg ccaatgccgt catcgccgtc aaccgcaacg acaagctggg
     4681 cctggccgag aagccgatgc tgctgatgga gttccacggc agcgagacga gcgtcgccga
     4741 gcaggccgcc accgtgcagg agctggccgc cgagcgcggg ggcaccgcgt tccagtgggc
     4801 gacgacgccc gaggagcgca cgcgcctgtg gacggcgcgc catcacgcgt tcctctccgg
     4861 cctgcaactg cgtccgggct gccgcgcggt gacgaccgac tgctgcgtgc cgatctccgg
     4921 cctggccgac gcggtcacgc aggcgctggc cgaggccgag gccgccggca tcccgcactt
     4981 cctcgtcggc cacgtcggcg acggcaactt ccacgtcgcc tatctcatcg accccgccag
     5041 cgcggcggac cgcgagacgg ccgagcgcct gaaccaccag ctggtgcagc gcgcgatcgc
     5101 ggccggcggc acctgcaccg gcgagcacgg catcgggctg cacaagcagg gcttcctgct
     5161 cgaggagacg ggcgccggcg cggtggcgat gatgcgcgag atcaagcgcg cgctcgatcc
     5221 gcacgacatc atgaacccgg gaaagatctt cggtccggcg tgacggcggc aaggcgcgag
     5281 ggatcgagcg ccgtcagcgg cggcggcctg tcacctgcgc ccggcctcag gtcgggcggc
     5341 ggcgcagcag gatgaccagc gcgccgagcg cgagcagcgc cggtgcgagg cggtgcgagt
     5401 ggtcgcgttc gcgatccagg ccggcgggca gcaccgagcc tgcgtcggtg gcggcgtcct
     5461 tgaacgcgag gtcgtccgcg gaccggtgcg gcgacccgtc gaagctgctc gcgacgggcg
     5521 gcgccaggct ggcgagctcg gccggcgcgt cgctcgcgtc gcccgcggcg tcggccacgc
     5581 gcagcgcgca ggcgagctcc agcggctggc cggggtggca tggcgtgtcc gcggcggcgg
     5641 cgctcgaggt gacggggtcg gccgccggat tggtggtcgt ccctgcggac gcggccagcg
     5701 ccacggccgc tgcgagcatt gcgatcttca gcatgcgtcg aaccttgtct ccctcgttct
     5761 tttgcggcgc caccttaccg cgactcgcgc gcggcgtcat caccggcgcc gacgaacggc
     5821 ggccgaatga tggaaaacga tgaggcggcc cgtcgtcgcg gatcgaacga tcagccatgc
     5881 ggcggcgtga ccgaccagcg acgcgtcgtc tcgcccttgc cgttgacgct ctccaggtga
     5941 acgtcgaagc cccacaggcg cgcgacgtgc ttgagcacct cctgcgagct ctcgtgcagc
     6001 ggacggtcgt tgtgctggct gtggcgcagc gtcagcgagc ggtcgccgcg caggttcacg
     6061 ttccagacct ggatgttggg ctcgcgcgag cccaggtcgt actggcgcga cagcgactcg
     6121 cgcagcgccc ggtagccgtt ctcgtcgtgg atggcggaga tctcgagctc gctgtccttc
     6181 tcgtcgtcga cgatcgcgaa gaagcggaag tcgcgcatca gcttgggcga caggtactgg
     6241 ccgatgaagc tctcgtcctt gaagttgcgc atcgcgtggt cgagcgtcgg cagccagggc
     6301 gagccggcga agtcggggaa ccactggcga tcctcgtcgg tcgggcactc gcagatgcgc
     6361 ttgatgtcgg tgtacatcgc gaagcccagc gcgtacgggt tgatgcccga gtagtggcgc
     6421 gaggtcacgg gcggctgggc gacgacgttg gtgtgcgact tcagccactc gatcatcatg
     6481 ccgtccgaca ggtagccgtc gtcgtacatc tggttgagca gccggtggtg ccagaacgtg
     6541 gcccagccct cgttcatcac ctgggtctgg cgctgcgggt agaagtactg cgcgatcttg
     6601 cgcacgatgc gcacgatctc gcgctgccag ggctccagca gcggcgcgtt cttctcgatg
     6661 aagtacagca ggttctcctg cggctcggcg gggaagcggc gctcctcgga ggccgagtcg
     6721 tcgctggccg cgcggcgcgg cagcgtgcgc cagaggtcgt tgacctgcag ctgcgcgtag
     6781 gcctcgcggt cctcgcgccg cgcctgttcc tgcgccagcg acagcttgct cgggcggcga
     6841 tagcggtcga cgccatggtt catcagcgcg tggcacgagt cgagcagctc ctcgacggcg
     6901 tcgatgccgt ggcgctcctc gcagcgcgcg acgtagtcct tcgagtagac gaggtagtcg
     6961 atgatcgacg acgcgtccgt ccacatccgg aacaggtagt tgcccttgaa gaagctgttg
     7021 tggccgtagg ccgcgtgcgc gatgaccagc gcctgcatcg ccatcgtgtt ctcctccatc
     7081 aggtaggaga tgcacgggtc ggagttgatg acgatctcgt aggccaggcc catctggccg
     7141 cgcttgtagt tcttctcggt ggcgatgaac tccttgccgt agctccagtg gcggtagttc
     7201 accggcatgc ccacgctggc ataggcgtcc atcatctgct cggaggtgat gatctcgagc
     7261 tggttcggat aggtgtccag gccgaagcgc tgcgcggtct cgcgaatcac ctcgtggtag
     7321 cgctcgatca tgtcgaacgt ccagtcgctg ggcgacggca gcggctgggc
//
LOCUS       DATCRO010000708         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_773689, whole genome shotgun sequence.
ACCESSION   DATCRO010000708 DATCRO010000000
VERSION     DATCRO010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773689"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..110
                     /locus_tag="VIN75_19210"
     CDS             6..110
                     /locus_tag="VIN75_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8976353.1"
                     /translation="MLHSVDGCTGCSLCALRCPFGAIEMRRAPDGVRS"
     gene            complement(308..1336)
                     /locus_tag="VIN75_19215"
     CDS             complement(308..1336)
                     /locus_tag="VIN75_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY8976354.1"
                     /translation="MTAAPDDSTLPWLAGPARDIATRQRGHALLLQGNAGAGVFELAL
                     RVARTWLCETDGADAPCGRCTACHLLATNSHPDFLALLPELTQVELGWSVGGNDAPEA
                     GDGEGKAKRKPSKEIRIEAVRDAIGWTQKSSSRGRAKVLLLFPADAMNLVASNALLKT
                     LEEPPAGVRLLLVAEDAERLLPTLRSRCQRLLFEGPTPDEALAWLQGQGAANAPVLLA
                     AAGGQPLAARRLAAEGFDAAAWVALPQRVARGAWEPPPGATLPRLREVLQKLCHDAMA
                     VAAGAAPRYFPAASLPPAAAWPKLADWSQSLARLARHDEHPFNAPLITDSLLAEAKAV
                     WARPRRAG"
     gene            complement(1333..1998)
                     /gene="tmk"
                     /locus_tag="VIN75_19220"
     CDS             complement(1333..1998)
                     /gene="tmk"
                     /locus_tag="VIN75_19220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8976355.1"
                     /translation="MNHFAGGAAGRFVTFEGIDGAGKSSHIEALADWARARGAEVVLA
                     REPGGTPLAEKLRGLFLHDAMDPLTEALLVFAARRDHLRQVIEPALARGALVLCDRFT
                     DSTFAYQGAGRGFDTAVLGQLEAWVQQGRQPDLTLWFDVDPAVAAQRRAAARDADRLE
                     REDAAFFARVRGGYASRAQAAPARFARIDASQPLDAVRADAVAALASRHWFPGPESNL
                     QSA"
BASE COUNT          265 a          754 c          772 g          259 t
ORIGIN      
        1 cggccgtgct gcattcggtc gacggctgca ccggctgcag cctgtgtgcg ctgcgctgcc
       61 cgttcggggc gatcgagatg cggcgcgccc cggacggggt caggtcttga atcttgcggg
      121 gtcaggtctt gaatgttgca gtgtcaggtc ttgccttttg catgacgtga cgaatgacga
      181 ttccgcggca agcggcgatg cgcagagccc ggccgtgcaa cattcaagac ctgaccccgc
      241 aaatttcaag acctgacccc catgcgcact gacccccatg cgacacccca gcgggcgtcg
      301 cgcgcgctca acccgcgcgc cgcggcctcg cccacaccgc cttcgcctcg gccagcaggc
      361 tgtcggtgat cagcggggcg ttgaacgggt gctcgtcgtg gcgcgcgagg cgggccagcg
      421 actgcgacca gtcggccaac ttgggccagg cggccgcagg cggcaggctg gcggcgggga
      481 agtagcgcgg ggcggcgccg gcggccacgg ccatggcgtc gtggcacagc ttctgcagca
      541 cctcgcgcag gcgcggcagc gtcgcgccgg gcggcggctc ccaggcgccg cgcgccacgc
      601 gctgcggcag cgcgacccag gcggcggcgt cgaagccctc ggcggccagg cggcgcgcgg
      661 ccagcggctg gccgccggcc gcggcgagca gcacgggcgc gttcgccgcg ccctgcccct
      721 gcagccaggc gagcgcctcg tcgggcgtcg gcccctcgaa caggaggcgc tggcaacggc
      781 tgcgcagcgt gggcagcagg cgctcggcgt cctcggccac cagcagcagg cgcacgccgg
      841 cgggcggctc ctccagcgtc ttgagcagcg cattggacgc cacaagattc atcgcgtcgg
      901 ccgggaacag cagcagcacc ttggcgcggc cgcgcgacga gctcttctgc gtccagccga
      961 tcgcgtcgcg cacggcctcg atgcggatct ccttgctggg cttgcgcttg gccttgccct
     1021 cgccgtcgcc cgcctcgggc gcgtcgttgc cgccgacgct ccagccgagc tcgacctgcg
     1081 tcagctcggg cagcagcgcc aggaaatcgg gatggctgtt ggtggccagc aggtggcagg
     1141 cggtgcagcg gccgcacggc gcgtcggcac cgtccgtctc gcacagccag gtgcgcgcga
     1201 cgcgcagcgc cagctcgaac acgcccgcgc cggcattgcc ctgcagcagc agcgcgtggc
     1261 cgcgctggcg cgtggcgatg tcgcgcgccg ggccggccag ccagggcagc gtggaatcgt
     1321 cgggggcggc ggtcatgcgg actgaaggtt cgattccggc ccggggaacc aatggcgcga
     1381 cgccagcgcc gcgaccgcat ccgcgcgcac cgcgtcgagc ggctgcgacg cgtcgatgcg
     1441 cgcaaagcgc gcgggcgccg cctgcgcgcg tgacgcgtac ccgccgcgca cgcgggcgaa
     1501 gaacgccgcg tcctcgcgct cgagtcggtc ggcatcgcgc gccgcggcgc ggcgctgcgc
     1561 ggcgacggcc ggatcgacgt cgaaccagag cgtgaggtcg ggctgccggc cttgctgcac
     1621 ccaggcctcg agctgcccga gcacggccgt gtcgaagccg cggcccgcgc cctggtaggc
     1681 gaaggtggaa tcggtgaagc ggtcgcacag cacgagcgcg ccgcgcgcca gcgccggctc
     1741 gatgacctgg cgcaggtggt cgcgccgcgc cgcgaacacc agcagcgcct cggtcagcgg
     1801 atccatcgcg tcgtgcagga acagcccgcg cagcttctcg gccagcggcg tgccgccggg
     1861 ctcgcgcgcc agcaccacct cggcgccgcg ggcgcgcgcc cagtcggcga gcgcctcgat
     1921 gtgcgaggac ttgccggccc cgtcgatgcc ttcgaacgtg acgaagcggc cggccgcgcc
     1981 gccggcgaag tggttcaccg gcgtcatttg tggccgccgc ggatgtactg gttcaccgcg
     2041 gcattatggg
//
LOCUS       DATCRO010000709         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_120390, whole genome shotgun sequence.
ACCESSION   DATCRO010000709 DATCRO010000000
VERSION     DATCRO010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120390"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VIN75_19225"
     CDS             <1..1288
                     /locus_tag="VIN75_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8976356.1"
                     /translation="AYSDLDSAVSAFQGGAFEYLPKPFDVPKAVELIRRAVSESLSEE
                     VRDEKAAQMPEMLGQAPAMQDVFRAIGRLSQSNVTVLITGESGSGKELVARALHRHSP
                     RGDHGSNGAFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQADGGTLFLDE
                     IGDMPFDLQTRLLRVLSDGQFYRVGGHQPIKSNVRVVAATHQNLEERVRQGVFREDLF
                     HRLNVIRLRLPPLRERIEDVPTLARFFLQKSAKELGVEPKRITDDALEAMKGFDFPGN
                     VRQLENVCHWLTVMAPAQVIESKDLPPELTAPRGSLPRAGATEPQTAPAADGHAAAAV
                     MGTDGAPGTSVLPAAHLAGVPAGSNWLGDLEFAARTLLDAGSPDVWDQLSRQFEGQLI
                     RTALALTRGRRIEAAQKLGIGRNTITRKIQELGLED"
     gene            1395..2150
                     /locus_tag="VIN75_19230"
     CDS             1395..2150
                     /locus_tag="VIN75_19230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8976357.1"
                     /translation="MPLLGFLDIAIAIFFAVHAVRTGRQMYWVFILIIAPFLGSLIYF
                     FAEYLPEMRHSTVARKSARVVKSIVDPNRELREARLAFERTPTVDNRSRLAEALLARG
                     DYADAVEQFQACASGPYARDVKFRRGLARAQLLAGRHADAAATLDALIADSPRDAGGD
                     AALWRAQALAQVDEARALEAFDHAMHVHNTTETQAAYGIFLASLGRDAQARPLLEGVL
                     HNARVGTPSSRELNREAIDQARAALKVVESRGG"
     gene            2263..3171
                     /locus_tag="VIN75_19235"
     CDS             2263..3171
                     /locus_tag="VIN75_19235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8976358.1"
                     /translation="MHLPTSALYAICILIWGTTWFAITAQIDVLAPTLGVAIRFGLAA
                     TVLLAFCVARRIPLRLPPKAQALVIAQGLTGFYASYVCVYEAERFVASGVVAVGYAAS
                     PLFGLALARLFLGTPMSRRVAIGGVAGVAGVVLIFWHEFTRLTASQQVVWGAELTMAS
                     VLLSSLSTIAAAGYHRLGVRGWAPLAWAMAWGAGAALAHALLAGTPWNWSWRPVFLGS
                     LGWLALLGSIVAFGAYYALVHRVGAAKAGYVGVLTPLVALMVSSVLENFLWTGLTLAG
                     IALAILGNAVALWPVARRPGPARRVA"
     gene            complement(3176..3952)
                     /gene="xth"
                     /locus_tag="VIN75_19240"
     CDS             complement(3176..3952)
                     /gene="xth"
                     /locus_tag="VIN75_19240"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8976359.1"
                     /translation="MKLATWNVNSLGIRLPHLLAWLAETQPDAVVLQETKLVDEKFPH
                     AQLKEAGWHVEFFGQKTYNGVALVSKLPASAIVRNIPGHADEQARVIAGTIGDVRIVG
                     GYFPNGQAPGSEKFAYKMAWLDALKGWLATELAANPKLVLMGDFNIAPEDRDVHDPVL
                     WYGQIHCTDEERAHFRGLCGLGLHDAFRLLEQPPKQWSWWDYRNLAFRKNQGLRIDHI
                     LVSEPLRAGVRACTIDKAQRKLERPSDHAPVIVEIDLPAA"
     gene            complement(3949..4086)
                     /locus_tag="VIN75_19245"
     CDS             complement(3949..4086)
                     /locus_tag="VIN75_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976360.1"
                     /translation="MPIASRPTLRRFVAAASVILPSRAASAHGGYDAMHDQHVTSPAA
                     P"
     gene            4257..4670
                     /locus_tag="VIN75_19250"
     CDS             4257..4670
                     /locus_tag="VIN75_19250"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY8976361.1"
                     /translation="MSKTPPPTAAELAILRVLWRDGPSTVRDVHAAVYADTDTGYTSA
                     LKLMQNMLAKGLLTRRDEARQHVYEAAVSERPTLNRLVGGWIDTAFSGSSLALAMQAL
                     DARPVDADELVQLKEMIARLERPEAPAGKPRKGRA"
     gene            4667..>5501
                     /locus_tag="VIN75_19255"
     CDS             4667..>5501
                     /locus_tag="VIN75_19255"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY8976362.1"
                     /translation="MSGFTLAYLARLEGWGLLAVALQGVLVLLSWQAWRRAVAAPEAR
                     HRLACLHLAALLVLPALTLAVLQAALLAAAQAPPKGPLVAALPSGADTLRVALGLGAA
                     AVWGLGALAMLLRLAREARALARLRDASAPACARVRASLRRSVRRGLVDVAPVVRAAA
                     VAGPQVAGWWRPWLLVPRDLAERLAPAELDAVLLHELAHLRRGDLRWNLLQRLVLALL
                     WFHPAAWSLQAGLARERERCCDALAVGHGASRAALARALVKLAEAAAQARTGATPRLV
                     LA"
BASE COUNT          687 a         1946 c         2051 g          817 t
ORIGIN      
        1 ggcctattcc gatctcgaca gtgcggtcag cgccttccag ggcggcgcgt tcgaatacct
       61 gcccaagccg ttcgacgtgc cgaaggcggt cgagctgatc cggcgcgcgg tgagcgaaag
      121 cctctccgag gaagtgcgcg acgagaaggc cgcgcagatg cccgagatgc tcggccaggc
      181 gccggcgatg caggacgtgt tccgcgcgat cggacggctg tcgcagagca acgtcacggt
      241 gctgatcacc ggcgagtcgg gctcgggcaa ggaactggtg gcgcgtgcgc tgcaccgcca
      301 cagcccgcgc ggcgaccacg gcagcaacgg cgcgttcgtc gcgatcaaca ccgcggccat
      361 cccgaaggac ctgctggagt ccgagctgtt cggccacgag cgcggcgcgt tcaccggcgc
      421 gcagacgatg cgccgcggcc gcttcgagca ggccgacggc ggcacgctgt tcctcgacga
      481 gatcggcgac atgccgttcg acctgcagac gcgcctgctg cgcgtgctca gcgacggcca
      541 gttctaccgc gtcggcggcc accagccgat caagtccaac gtgcgcgtgg tcgccgcgac
      601 gcaccagaac ctcgaggagc gggtacgcca aggggtattc cgcgaggacc tgttccaccg
      661 cctcaacgtg atccgcctgc gtctgccgcc gctgcgcgag cgcatcgagg acgtgccgac
      721 gctcgcgcgc ttcttcctgc agaaaagcgc caaggaactg ggcgtcgaac ccaagcgcat
      781 caccgacgac gcgctcgagg cgatgaaggg attcgatttc cccggcaacg tgcgccagct
      841 cgagaatgtc tgccactggc tcaccgtgat ggcgccggcc caggtgatcg agagcaagga
      901 cctgcccccc gagctgaccg ccccgcgcgg cagcctcccg cgcgccggcg cgaccgagcc
      961 gcagaccgcg ccggcggcgg acggccacgc cgccgcggcc gtcatgggca cggatggggc
     1021 gccaggcacc agcgtgttgc ccgccgccca tctggcgggc gtgcccgccg ggagcaactg
     1081 gctgggcgac ctcgagttcg ccgcgcgcac cttgctggac gccggttcgc ccgacgtctg
     1141 ggaccagctc agccgccagt tcgaggggca gctgatccgc acggcgctgg cgctcacgcg
     1201 cggccgccgg atcgaggccg cgcagaagct gggcatcggg cgcaacacga tcacgcgcaa
     1261 gatccaggag ctcgggctcg aggactgagt ttcccgcgcc cgcgggcccg cgggcgtcac
     1321 gcgcccccgg gcgcgcgcgg gggggcttca cgcacgccgc ctacaattcc acccacaacg
     1381 ttgggaggaa tctcgtgccg cttcttggct tcctggacat cgccatcgcg atcttctttg
     1441 ccgtccacgc cgtgcgcacc gggcggcaga tgtactgggt gttcatcctg atcatcgcgc
     1501 cgttcctggg cagcctgatc tacttcttcg ccgagtacct gcccgagatg cgccactcga
     1561 ccgtggcccg caagtcggcg cgcgtcgtca agtccatcgt cgacccgaac cgcgagctgc
     1621 gcgaggcgcg cctcgccttc gagcgcacgc ccacggtcga caaccgctcg cgcctggccg
     1681 aggcgctgct ggcgcgcggc gactacgccg acgccgtcga gcagttccag gcctgcgcca
     1741 gcggcccgta cgccagggac gtgaagttcc gccgcgggct ggcgcgcgcg caactgcttg
     1801 ccggccgcca cgccgacgcc gccgccacgc tggacgcact gatcgccgac tccccgcgcg
     1861 acgccggcgg cgacgccgcg ctgtggcgcg cgcaggcgct ggcccaggtc gacgaggccc
     1921 gcgcgctcga ggccttcgac cacgccatgc acgtgcacaa caccaccgag acgcaggccg
     1981 cctacggcat cttcctggcc agcctgggcc gcgacgcgca ggcgcgaccg ctgctggagg
     2041 gcgtgctgca caacgcccgc gtcggcacgc cgtcgtcgcg cgagctcaat cgcgaggcga
     2101 tcgaccaggc gcgcgcggcg ctcaaggtgg tggagtcgcg cgggggctga acgcgtcgcc
     2161 ggcggcgggt tgccagcggc agcgtgaggt gcaaagccgc gcaccgaaag cgggcctttg
     2221 cccggcgagg gtcggaggat gcgtggcaaa ctgcgcggcc ccatgcacct gccgacttcc
     2281 gccctctacg cgatctgcat cctgatctgg ggcacgacct ggttcgccat caccgcccag
     2341 atcgacgtcc tcgcgccgac gctgggcgtg gccatccgct tcggcctggc cgcgacggtg
     2401 ctgctcgcgt tctgcgtcgc gcgccgcatc ccgctgcggt tgccgcccaa ggcgcaggcg
     2461 ctggtgatcg cccagggcct gacgggcttc tacgccagct atgtgtgcgt ctacgaggcc
     2521 gagcgcttcg tggccagcgg cgtcgtcgcg gtggggtatg ccgcgtcgcc gctgttcggc
     2581 ctggcgctcg cgcggctgtt cctcggcacg ccgatgtccc ggcgcgtggc gatcggcggc
     2641 gtcgcgggcg tcgccggcgt ggtgctgatc ttctggcacg agttcacgcg gctgacggcc
     2701 tcgcagcagg tggtatgggg cgcggagctg acgatggcct cggtgctgct gtccagcctg
     2761 tcgacgatcg ccgcggcggg ctaccaccgg ctcggcgtgc gcggctgggc gccgctcgcc
     2821 tgggcgatgg catggggcgc gggcgccgcg ctcgcgcatg cgctgctggc gggcacgcct
     2881 tggaattggt cgtggcggcc cgtgttcctg ggttcgctcg ggtggctggc cctgctgggc
     2941 tccatcgtgg ccttcggcgc ctactacgcg ctggtgcatc gcgtcggcgc ggcgaaggcg
     3001 ggctacgtgg gcgtgctcac gccgctggtg gcgctgatgg tgtccagcgt gctcgagaac
     3061 ttcctgtgga cgggcctgac gctggccggc atcgcgctgg cgatcctggg caacgcggtc
     3121 gcgctgtggc ccgtcgcccg gagaccgggg cctgccaggc gcgtggcctg acgcttcagg
     3181 ccgcgggcag gtcgatctcg acgatcaccg gcgcgtggtc gctgggacgc tcgagcttgc
     3241 gctgcgcctt gtcgatcgtg caggcgcgca cgccggcgcg cagcggctcg ctgaccagga
     3301 tgtggtcgat gcgcagcccc tggttcttgc ggaacgcgag gttgcggtag tcccaccagc
     3361 tccactgctt gggcggctgc tcgagcaggc ggaaggcatc gtgcaggccc aggccgcaca
     3421 ggccgcggaa gtgggcgcgc tcctcgtcgg tgcagtggat ctggccgtac cacagcaccg
     3481 gatcgtgcac gtcgcggtct tccggggcga tgttgaagtc gcccatcagc accagcttcg
     3541 gattggcggc gagctcggtg gcgagccagc ccttgagcgc gtcgagccag gccatcttgt
     3601 aggcgaactt ctcgctgccg ggtgcctggc cgttcgggaa gtagccgccc acgatgcgca
     3661 cgtcgccgat ggtgcccgcg atcacgcgcg cctgttcgtc ggcatggccg gggatgttgc
     3721 gcacgatggc gctggccggc agcttcgaca ccagcgccac gccgttgtac gtcttctgcc
     3781 cgaagaactc gacgtgccag ccggcctcct tgagctgcgc gtgcggaaac ttctcgtcga
     3841 ccagcttggt ctcctgcagc accaccgcgt cgggctgcgt ctcggccagc caggccagca
     3901 ggtggggcag gcggatgccc agggagttga cgttccaggt ggcgagtttc atggtgctgc
     3961 tggcgaagtg acgtgctggt cgtgcatcgc atcgtagccg ccatgggcgc ttgcggccct
     4021 tgacggcagg atgaccgacg cggcggcgac gaaacggcgc agcgtcggac gcgaggcgat
     4081 gggcatgggg cccgattgtg cccggcggcg cgggcgcccc gatcgggcgc cgcccgcgcc
     4141 ggggcagctg cggtggaaga cgatggtggg tctggggccc tttccctcgc cttgccggcg
     4201 gccaagtgcc acttgtgcgc atggcgcgga gtggatggcg cgcgcacaat gagcccatga
     4261 gcaagacgcc gccgcccacc gccgccgaac tggccatcct gcgcgtgctg tggcgcgacg
     4321 ggccgtccac ggtgcgcgac gtgcacgcgg ccgtgtacgc cgacacggac accggctaca
     4381 cgagcgccct caagctcatg cagaacatgc tggccaaggg gctgctcacg cgcagggacg
     4441 aggcgcgcca gcacgtctac gaggcggcgg tgtccgagcg acccacgttg aaccgactgg
     4501 tcggtggctg gatcgacacc gcgttctcgg gctcgtcgct ggcgctggcg atgcaggcgc
     4561 tcgacgcgcg gccggtcgac gcggacgaac tggtgcagct caaggagatg atcgcgcgcc
     4621 tcgaacgccc cgaggcgccg gcgggcaagc cgcgaaaggg tcgcgcgtga gcggcttcac
     4681 gctggcctat ctggcgcgac tcgaaggctg gggcctgctg gcggtcgcgc tgcagggcgt
     4741 gctggtgctg ctgtcgtggc aggcgtggcg ccgggccgtc gcggcgccgg aagcgcgtca
     4801 tcggctggcg tgcctgcacc tggccgcgtt gctggtgctg cccgcactga cgctggcggt
     4861 gctgcaggcg gccctgctcg cggcggcgca ggcgccgccg aagggcccgc tggtcgcggc
     4921 gctgccgtcc ggggccgaca cccttcgtgt cgcgctcggg ctcggggccg cggcggtgtg
     4981 ggggctcggc gcgctggcga tgctgctgcg gctcgcgcgc gaggcgcggg cgctcgcgcg
     5041 cctgcgtgac gcgtccgcgc ccgcctgcgc gcgggtgcgt gcgtcgctgc ggcgctcggt
     5101 ccggcgcggg ctcgtcgatg tcgcacccgt cgtgcgcgcg gccgccgtcg ccggtcccca
     5161 ggtggcgggc tggtggcgcc cgtggctgct ggtgccgcgc gacctggccg agcggcttgc
     5221 gccggccgag ctcgacgccg tgctgctgca cgagctggcc catctgcgcc gtggcgacct
     5281 gcgctggaac ctgctgcagc gcctcgtgct ggcgctcctg tggttccatc cggccgcctg
     5341 gtcgctgcag gccggtctcg cgcgcgagcg cgaacgctgt tgcgatgcac tggccgtcgg
     5401 gcatggcgcg tcccgggcgg cactggcgcg ggcgctggtg aaactggccg aagcggcggc
     5461 gcaggcgcga acgggcgcga cgccgcggct cgtgctggcg g
//
LOCUS       DATCRO010000710         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_154605, whole genome shotgun sequence.
ACCESSION   DATCRO010000710 DATCRO010000000
VERSION     DATCRO010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154605"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1063)
                     /locus_tag="VIN75_19260"
     CDS             complement(38..1063)
                     /locus_tag="VIN75_19260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8976363.1"
                     /translation="MTLAPTTPATGPGASARDAGIGMVVIGRNEGARLVRCLEACARE
                     GGAVVYVDSGSTDGSPDAARRLGATVVALDMSIPFTAARARNEGFSALMRLHPGTARV
                     QFLDGDCELAAGWLAAANAFLDAHPEIAAVCGRRLERFPEASVYNRLCDIEWNTPVGE
                     ALACGGDVLIRSQPLAAAGGYRDDLIAGEEPELCVRLRAAGWKIWRLGHDMTWHDAAM
                     TRWMQWWRRNVRSGHAFAEGAALHGAPPERHFVAQTRRALLWGGALPLVLLVATLAWW
                     PLAALWLVYPLQWLRVALKLQRAGQPIPWTQSAFFLQGRFAEAVGALRFWWGRTTERR
                     SAIIEYK"
     gene            1341..2429
                     /locus_tag="VIN75_19265"
     CDS             1341..2429
                     /locus_tag="VIN75_19265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8976364.1"
                     /translation="MKITFVCPPLNMTGGIRVVAIHAKYLQSFGHEVQVVAPPRAVRG
                     RLADWRRKLRLPPKVMRPQPPSHMDGTGVPTKLLERPRPVLASDVPDADVIVATWWET
                     AEFVAAMPASKGRKVYFVQGHEVFKGQPIDRVVATYRSDLRKVAVARWLAEAIERDGN
                     RQGCTIVPNAVDRSLFFAPPRERNARPRLGTLFSENDNKSFDIALAVIAAVRKEMPEV
                     EVVALGSDAPARYRDRLEGIALTLQAPQDRLREVYASCDVWLTCSKTEGFNLMAMEAM
                     ACRTPVVSTRTGWPVEAIVDGVNGALADVDDVAGLAAGVLRILRLDAARWREVSEGAA
                     RTVADSSWERSARLFEAALIGNEKIAAA"
BASE COUNT          479 a         1151 c         1093 g          458 t
ORIGIN      
        1 ctccatgcgc gcggcctgca gcccgcgtgc gcccgattca cttgtattca atgatggcac
       61 tcctgcgttc ggtcgtgcgg ccccaccaga accgcaaggc gcccaccgcc tcggcgaagc
      121 ggccctgcag gaagaaggcg gactgcgtcc agggtatcgg ctggcccgcg cgctgcaact
      181 tgagcgccac gcgcagccat tgcagcggat agacgagcca cagcgccgcc agcggccacc
      241 atgcgagcgt ggccacgagc agcacgagcg gcagcgcgcc gccccacagg agcgcgcgcc
      301 gcgtctgcgc gacgaaatgg cgttccggcg gcgcgccgtg cagcgccgcg ccctcggcga
      361 aggcatggcc cgagcgtacg ttgcggcgcc accattgcat ccagcgcgtc atcgcggcgt
      421 catgccaggt catgtcgtgg ccgaggcgcc agatcttcca gcccgccgcg cgcaagcgca
      481 cgcacagctc cggttcttcg ccggcgatca ggtcgtcgcg atagccgccc gccgcagcaa
      541 gcggttgcga acgtatcagg acgtcaccac cgcaggccag cgcctcgccg acgggcgtgt
      601 tccattcgat gtcgcacagg cggttgtaga ccgacgcctc tggaaatctt tccagtctgc
      661 ggccgcacac tgcggcaatt tcgggatgcg cgtcgaggaa cgcgttggcg gcggccaacc
      721 agcccgcggc cagctcgcag tcgccatcga ggaactgcac gcgcgccgtg cccggatgca
      781 ggcgcatcag cgcggaaaac ccctcgttgc gggcacgcgc cgccgtgaac gggatcgaca
      841 tgtccagggc caccaccgtc gcgccgagcc gccgcgcggc gtcgggcgag ccgtcggtcg
      901 acccgctgtc gacgtagacg accgcgccgc cctcgcgtgc gcaggcctcg aggcagcgca
      961 cgaggcgcgc gccctcgttg cggccgatga cgaccatgcc gatcccggcg tcgcgcgcgg
     1021 acgcgccggg gccggtcgcg ggggtcgtcg gggcaagggt catggaggcg gtaatttcgg
     1081 gttccggcac ggcgcttggg ccgtcgatcg acagccggta gcaattttgc tgccgagttg
     1141 gtgcgggccg cgcatccggc cgcttccggc tccccggatc gggggatcgc gacgcctcgc
     1201 ggcgcctccc cggcgacgca ggcccggcat cctatacgat ggcccccgcc cgcccgggcc
     1261 cgcgcggaac gccgcttgct tttgcaggcg ccgcccctcg ccttccggcg ggcctcgccc
     1321 actcccgcgc cctgccccga atgaagatca cctttgtctg cccgcccctg aacatgaccg
     1381 gcggcattcg cgtcgtcgcc atccacgcga agtacctgca atcgttcggg cacgaggtgc
     1441 aggtcgtggc gccgccgcgc gccgtgcgcg ggcggctggc cgactggcgt cgcaagctgc
     1501 gcctgccgcc caaggtcatg cggccgcaac cgccctcgca catggacggc accggcgtgc
     1561 ccacgaagct cctggagcgt ccgcgcccgg tgctcgcgtc cgacgtgccc gacgccgacg
     1621 tgatcgtcgc gacctggtgg gagaccgccg aattcgtcgc cgcgatgccg gcctccaagg
     1681 gacgcaaggt ctacttcgtg cagggccacg aggtgttcaa gggccagccg atcgaccgcg
     1741 tggtcgccac gtaccgctcg gacctgcgca aggtcgccgt cgcgcgctgg ctcgccgagg
     1801 cgatcgagcg cgacggcaac cgccagggct gcacgatcgt ccccaacgcg gtcgaccgct
     1861 cgctgttctt cgcgccgccg cgcgagcgca acgcgcggcc gcgcctgggg acgctgttct
     1921 cggagaacga caacaagagc ttcgacatcg cgctggccgt gatcgccgcg gtgcgcaagg
     1981 agatgcccga ggtcgaggtc gtggcgctcg gctccgacgc gccggccagg taccgcgacc
     2041 gcctcgaggg catcgcgctg acgctgcagg ccccgcagga caggctgcgc gaggtctacg
     2101 cgagctgcga cgtgtggctc acgtgcagca agaccgaagg cttcaacctg atggcgatgg
     2161 aggccatggc gtgccgcacg ccggtggtct ccacgcgcac gggctggccg gtcgaggcca
     2221 tcgtcgacgg cgtcaacggc gcgctggccg acgtggacga cgtggccggc ctcgcggcgg
     2281 gcgtgctgcg catcctgcgg ctggacgcgg cgcgctggcg cgaggtctcc gaaggcgcgg
     2341 cgcgcaccgt ggccgattcg agctgggaac gctccgcgcg cctgttcgag gcggcgctaa
     2401 ttggaaacga aaagatagca gcggcctgat gcctccggcc gtatcgcgcg ggagcgcggt
     2461 gcggcgtcac gcgatcgtca tgtcgcgcgt ccgtcgaggc gcgcagcgcc gtcgcgtccc
     2521 ggtcaccgcg cgcattcgca tggggaaatc ggccgatccg cgcaggtcgg acgttcagga
     2581 ttgcgtgtcc aatggataca tcgagatacc tcactgggtg tcgcgtctct cgatgtgacg
     2641 gcaccatgcc cctgcacttt ctcgagggga ggtcgagggg gtttcttgat cgctcgccta
     2701 cggccgagcg aagaccgggt gtgcgcccga attagttctc gaacaccgct cttctcgcga
     2761 gttcgacgga ctcaacgcgc ctgcgtcgcc gccgaagaac tgggcatggc gacacggaaa
     2821 cgcgcaagtg tttccaagcc ggcacgagtc atccgaagac atctcgttac acggcacccg
     2881 ttcatcgcgg cagtcgctga ccgatgagca cggaatgccg tacggccgcc tgcgatcgat
     2941 tgcgggagtg cctgcatcgc gcctggattc agaccacaat ctccatgtct caaagcaatc
     3001 ccagtctccg catggcaacc agcattgccc tggccctgac caccgccgcc atcaccgctt
     3061 gcggtggcgg gggctccggc gacggctcga ccccgacggt cgaagccgtg accacggttt
     3121 ccggcgccgc cgacgcgcca tcgtcgtccg cgagcgaggc gtacgccacg accaaccagg
     3181 c
//
LOCUS       DATCRO010000711         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_367856, whole genome shotgun sequence.
ACCESSION   DATCRO010000711 DATCRO010000000
VERSION     DATCRO010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367856"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1431
                     /locus_tag="VIN75_19270"
     CDS             37..1431
                     /locus_tag="VIN75_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8976365.1"
                     /translation="MALLAPWAVPAQAQSAPPSAAAAAAAPGPQQFTLANGMTLIVQP
                     DRRSPTAVQMVWVRVGSIDEVDGTSGVAHALEHMMFKGTKDIKPGEFSRRVAALGGQE
                     NAFTTRDYTGYYQQIPVGSLEQVMKLESDRFANNQWPDDEFKREIEVVKEERRLRTED
                     QPRALLGEQQNAAVFTASPYHRPVVGWMSDLDAMTPDDARAFFRQWYVPANAVVVVAG
                     DVDVAQVRAMAEKYYGRIPARAVPARKPRIEPAQRGIRRIELKAPAEQAYVSLAYRVP
                     QLASIDDVNSDAWALVVLSAVLDGYSGARLDRALTQGPDRVADSAGAYSGFIGRGPQL
                     FVLDGVPAAGKSAEVVEAALRAQVARIAKEGVGEAELARVKTQWVASETYKRDSVMAQ
                     ARELGSNWIQGLPLDASERIVARLQSVTAAQVQAVAAKYFGDDQLTVATLRPLPPESK
                     PRGRGFAAPAGEMR"
     gene            1447..>2090
                     /locus_tag="VIN75_19275"
     CDS             1447..>2090
                     /locus_tag="VIN75_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8976366.1"
                     /translation="MQNTLMKTARRALLAGVVAVAGIPGAQAALPIQHWTLANGAKIY
                     LVSTNALPIVDAQLDFDAGSRRDPAAQAGLASVTATMVEKGVRAGRNGEPALDQNALG
                     EAWADLGASFDVSAGTDRTSYSLRSLSDPALLNKAVALAAREIGEPSFPDDVWQRERE
                     RINAAIKEANTKPATVAGRAFAADVYGVHPYGQEVKAETLARIDTAAMRQRYQQL"
BASE COUNT          323 a          793 c          702 g          272 t
ORIGIN      
        1 ccgccacgcc gtgctgcgtc tggtgcggtg ctggcgatgg cgctcctggc gccgtgggcc
       61 gtgcctgccc aggcccaatc cgccccgcct tccgccgccg cagcagcagc ggctcccggt
      121 ccccagcaat tcacgctcgc caacggcatg acgctgatcg tccagcccga ccggcgctcg
      181 cccacggcgg tgcagatggt ctgggtccgc gtcggctcca tcgacgaggt cgacggcacc
      241 tccggcgtgg cccatgcgct cgaacacatg atgttcaagg gcaccaagga catcaagccc
      301 ggcgagttct cccgccgcgt cgccgccctc ggcggccagg agaacgcctt caccacccgc
      361 gactacaccg gctactacca gcagattccg gtcggcagcc tcgagcaggt gatgaagctc
      421 gaatcggacc gcttcgccaa caaccagtgg cccgacgacg agttcaagcg cgagatcgag
      481 gtggtcaagg aagagcgccg cctgcgcacc gaagaccagc cgcgcgccct gctcggcgag
      541 cagcagaacg ccgccgtctt caccgcctct ccctatcacc gccccgtggt cggctggatg
      601 agcgacctcg acgccatgac gcccgatgac gcgcgcgcgt tcttcaggca gtggtacgtg
      661 cccgccaacg ccgtcgtcgt ggtggccggc gacgtcgacg tggcgcaggt gcgcgccatg
      721 gccgagaagt actacggccg gatccccgcg cgcgccgtgc ccgcgcgcaa gccgcgcatc
      781 gagccggcgc agcgcggcat ccgccgcatc gagctgaagg cgccggccga gcaggcctac
      841 gtgtcgctcg cctaccgcgt gccgcagctc gccagcatcg acgacgtgaa cagcgacgcc
      901 tgggcgctgg tggtgctctc ggccgtgctc gacggctatt ccggcgcccg gctcgaccgc
      961 gcgctgaccc aggggcccga ccgcgtcgcc gattcggccg gtgcgtactc gggcttcatc
     1021 ggacgcggcc cgcagctctt cgtgctcgac ggcgttccgg ccgccggcaa gagtgcggag
     1081 gtggtcgagg ccgcgctgcg cgcccaggtc gcgcgcatcg ccaaggaggg cgtgggcgag
     1141 gccgaactgg cccgcgtcaa gacccagtgg gtggcgagcg agacctacaa gcgcgactcc
     1201 gtgatggcgc aggcgcgcga actcggcagc aactggatcc agggccttcc gctggacgcc
     1261 agcgagcgca tcgtcgcgcg gctgcaatcc gtcacggccg cccaggtgca ggccgtggcc
     1321 gccaaatact tcggcgacga ccagctcacc gtcgccacgc tgcgtcccct gccgcccgag
     1381 tccaagccgc gcggccgcgg tttcgccgcc cccgcgggcg agatgcgctg aaaggtcacc
     1441 attcccatgc agaacactct catgaagacc gcgcgccgcg ccctgctcgc cggcgtcgtc
     1501 gccgtggcgg gcatcccagg cgcgcaggcc gcgctgccta tccagcactg gacgctggcc
     1561 aacggcgcca agatctacct ggtgtcgacc aacgcgctgc cgatcgtgga tgcgcaactc
     1621 gacttcgatg ccggcagccg ccgcgaccct gccgcgcagg ccggcctggc cagcgtgact
     1681 gcgaccatgg tcgagaaggg cgtgcgcgcc ggcaggaacg gcgagcccgc gctcgaccag
     1741 aacgccctgg gcgaggcctg ggccgatctg ggcgcgagct tcgacgtgag cgcgggcacc
     1801 gaccgcacca gctattcgct gcgctcgctg tccgatccgg cgctgctgaa caaggcggtg
     1861 gcgctcgcag cacgcgagat cggcgagccc tccttccccg acgacgtatg gcagcgcgag
     1921 cgcgagcgca tcaacgccgc catcaaggag gccaacacca agcccgccac cgtggccggc
     1981 cgcgcttttg cggccgacgt ctacggcgtg catccctacg gccaggaagt gaaggcggag
     2041 acgctcgcgc gcatcgacac cgccgccatg cgccagcgct accagcagct
//
LOCUS       DATCRO010000712         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_480908, whole genome shotgun sequence.
ACCESSION   DATCRO010000712 DATCRO010000000
VERSION     DATCRO010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480908"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VIN75_19280"
     CDS             complement(<1..834)
                     /locus_tag="VIN75_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976367.1"
                     /translation="MSDSSNKRFEDLVGPGESLRVMSALKRGASRRDVLAMLMAGGMQ
                     VTLAGSLAGMAVSAHAQTPRRGGRIRVAGATAAATDTLDPAKQSNQTDYSRCNMVYNG
                     LTSLDGSLTPQPALAESFTTKDAKVWVFTLRKGVVFHDGKPLAPADVVFSVMRHKDAA
                     TASKAKVLADQIESVKATGPNEVTIVLSAPNADLPVILGTYHFHIVKDGTTDFNAGIG
                     TGPYKLKEFKPGVRVVAVRNENYWKPGRPYLDEIEYVGIADETARVNALLAGELDLIG
                     TV"
     gene            complement(918..2210)
                     /locus_tag="VIN75_19285"
     CDS             complement(918..2210)
                     /locus_tag="VIN75_19285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8976368.1"
                     /translation="MKLDSYWTDSAAAFTPAAHALPEQVDVAIVGGGFTGLSAALALA
                     KRGARVAVLEAGARVAAEASGRNGGHVNNGLAVDYADVAAKVGVEKARAWYRAYDAAV
                     DTVARLVRDEAIDCDFLRHGKLKLATRPSQMEALERSARRLIDDGVDIDVELLDAARV
                     RAEVQSERFHGGLLYKRSGQMHMGRFAQGLAEAAQRHGAQIHTATTVNRLERLGQGHA
                     HRLHTSRGTVTAQQVLLATGATRHGGYGSFGWLRRRIVPIGSFIVVTEPLGAQRAQAL
                     LSARRTYTTIANIHHYFRLTADHRLVFGGRARFAISSPQSDAASGEILRAGLAETFPQ
                     LGAVRLDYCWGGLVDMTQDRLPHAGERDGLYYAMGYSGHGTQMSVHMGERMAAVMNGD
                     AGANPWRDRAWPAIPGHFGPPWFLPAVGLYYSLKDKLA"
BASE COUNT          319 a          764 c          802 g          355 t
ORIGIN      
        1 gaccgtgccg atcaggtcca gctcgccggc cagcagcgcg ttgacgcgcg ccgtctcgtc
       61 ggcgatgccc acgtactcga tctcgtccag gtacgggcgg ccgggcttcc agtagttctc
      121 gttgcgcacg gcgacgacgc gcacgccagg cttgaactcc ttgagcttgt acgggccggt
      181 gccgatgccg gcgttgaagt cggtggtgcc gtccttgacg atgtggaagt ggtaggtgcc
      241 caggatcacc ggcaggtcgg cgttgggcgc gctcagcacg atcgtcactt cgttcggacc
      301 cgtggccttc acgctctcga tctggtcggc gagcaccttg gccttggagg cggtggccgc
      361 gtccttgtgg cgcatcaccg agaacaccac gtcggcgggc gcgagcggct tgccgtcgtg
      421 gaacaccacg cccttgcgca gcgtgaagac ccagaccttg gcatccttgg tggtgaacga
      481 ttcggccagc gcgggttgcg gcgtgaggct gccgtcgagc gaggtcaggc cgttgtagac
      541 catgttgcag cgcgagtaat cggtctggtt cgactgcttg gccggatcga gcgtgtcggt
      601 tgccgcagca gtggcgcccg ccacgcggat gcgcccgccg cgcctcggcg tctgcgcgtg
      661 ggcggacacc gccatgcccg cgaggctgcc cgccagcgtg acctgcatgc cgccggccat
      721 cagcatcgca agcacgtcgc gtcgcgacgc gccgcgtttc aacgcgctca tcacgcgaag
      781 gctttcgccg ggaccgacga ggtcttcgaa cctcttgttg ctgctgtcgc tcatgatggg
      841 ttactcctgt gtggccggtg accgggggtt gaagaacaaa cgaacgctga acgaaacggg
      901 tcggactcgc tgcgcgctca ggccagcttg tccttgagcg agtagtagag gccgacggcc
      961 ggaaggaacc acggcgggcc gaagtgcccg ggaatggcgg gccaggcgcg gtcgcgccag
     1021 gggttggcgc cggcatcgcc gttcatcacg gcggccatgc gctcgcccat gtgcaccgac
     1081 atctgcgtgc cgtggccgct gtagcccatg gcgtagtaga ggccgtcgcg ctcgccggcg
     1141 tgaggcaggc ggtcctgcgt catgtcgacc agcccgcccc agcagtagtc caggcgcacg
     1201 gcacccagtt gcggaaaggt ttccgcgagc ccggcgcgca ggatctcgcc gctcgcggca
     1261 tccgactggg ggctggagat cgcgaatcgc gcgcgcccgc cgaacaccag ccggtgatcg
     1321 gccgtgagcc ggaaatagtg atggatgttc gcgatcgtcg tgtacgtgcg ccgcgccgac
     1381 agcagcgcct gtgcgcgctg cgcgccgagc ggctcggtca cgacgatgaa gctgccgatc
     1441 ggcacgatgc gccggcgcag ccagccgaag ctgccgtagc cgccatggcg cgtggcaccc
     1501 gtggccagca ggacctgctg cgcggtgacg gtgccgcggg aggtatgcag ccggtgcgca
     1561 tggccctggc cgagccgctc gagccggttc accgtcgtgg cggtgtggat ctgcgcgccg
     1621 tggcgctgcg ccgcctcggc caggccctgg gcgaagcggc ccatgtgcat ctggccgctg
     1681 cgcttgtaga gaaggccgcc gtggaagcgc tcgctctgca cctcggcgcg cacccgcgcc
     1741 gcatccagga gctcgacgtc gatgtccacg ccgtcgtcga tcaggcgccg agcgctgcgc
     1801 tcgagcgcct ccatctggct gggccgcgtg gcgagcttga gcttgccgtg ccgcaggaaa
     1861 tcgcagtcga tcgcctcgtc gcgtaccagc cgcgcgacgg tgtccactgc ggcgtcgtag
     1921 gcccgatacc aggcgcgggc cttttcgacg ccgaccttgg cggccacatc ggcatagtcc
     1981 acggccagcc cgttgttgac atgcccacca ttgcggcccg aggcctccgc cgccacacgc
     2041 gcaccagcct ccaggacagc gacgcgcgcg ccgcgcttcg ccagcgccag cgccgcggac
     2101 aggcccgtga acccgccgcc gacgatcgcg acgtcgacct gttccggcag cgcatgcgct
     2161 gcaggcgtga aggctgcggc gctatcggtc cagtaggagt cgagcttcat ggcggggcgg
     2221 gcaacggggc gaggcaggga
//
LOCUS       DATCRO010000713         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_570650, whole genome shotgun sequence.
ACCESSION   DATCRO010000713 DATCRO010000000
VERSION     DATCRO010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570650"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VIN75_19290"
     CDS             <1..1941
                     /locus_tag="VIN75_19290"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY8976369.1"
                     /translation="VQGRVAYANVTDAMVPAALGDTVLGVVGLQTVHLARVHAKRADA
                     THVAASSTQAVTAVSIPSFPVIYGASDLPSATSATIGIITSGSLTQTIKDLKSFATSA
                     GYPSPPVTTTVVGSASSGTSGIDEWNMDSQAALAAAGGSIKSLVLYNVGSLSDSDLTQ
                     GYNRAVTDNTARAINVSLGACETDEVSIEPTQDQIFETALTQGQMFSVSSGDSGAFEC
                     GTAAGKAQSYPAVSPYVMAVGGTTLSTSNGAWAGETAWSCTDASNCQQSGSGGAGGGP
                     SLTEKAPSWQVASGVLGTSTMRGVPDIAYDASPSSGALVLVDGSPVQIGGTSLAAPLW
                     AGFWARIQSMDGNSLPFPAQTLYQGGASHPEWFHDITSGNQGYAATTGWDYASGWGSI
                     QVDHFATAFCECTPSQLTPDFSFTMNGLTASFVNSSTDIGSTINSYRWNFGDGTSDTV
                     ANPTHTYPAFATYTATLTVTDTMGNTQSKTEGVPVVQPPPVQPILANGGFESGSTGWT
                     ATSGVICASTACAGESAHGGAGFAWLDGYGSAHTDTLAQTVKLPSGYKNARMTFFLHV
                     DTAEATTSTQYDKLAVSVTSGSTSTTIGTYSNLNAAPGYAPRTLDLTPWLGKTVTIRF
                     SGTEDSSRQTSFVLDDIAITLQ"
     gene            complement(1989..2306)
                     /locus_tag="VIN75_19295"
     CDS             complement(1989..2306)
                     /locus_tag="VIN75_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976370.1"
                     /translation="MQVHEILAATLLAASAFGAMSQELDPGETLQAKNLSAPRLHIAP
                     SNPRDLSHVIAAVRATESDAPATVAVADTSWASRHFKDRYARKWLRGDKRPATQVAAG
                     QAG"
     gene            complement(2554..3012)
                     /locus_tag="VIN75_19300"
     CDS             complement(2554..3012)
                     /locus_tag="VIN75_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8976371.1"
                     /translation="MKDIIHRIGIRGPAARVYDALATIPGLAGWWTEETTGESKVGGV
                     VKFVFHLENGELLGGFDMDVKELSPGDKVRWQVKDGPPEWIGTAIEFSLSRQDDFTIV
                     LFSHRGWREEVEFMAHCSTKWATFLVSLRDFVETGKGQPAPNDLRLGNWH"
     gene            3096..3797
                     /locus_tag="VIN75_19305"
     CDS             3096..3797
                     /locus_tag="VIN75_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8976372.1"
                     /translation="MSNKVGANPGGPCRPNISGATEVESAPFEQPNRGSFQMSQANPS
                     VDPWFAKARKWQVEIARLREILLGCDLGEELKWGKPTYTADGANIVLIMPLKDTCTLL
                     FMKGALMKDAKGLLVQPGENSQSARQMRFTSAAEITKLKATIRSYVKEAVELEKSGAK
                     VDFKKSSDLIYPKEFQDRLDRNTALREAFFALTPGRQRQYHLHFTGAKQSATRESRVE
                     KAIPLILDGMGLNDQ"
     gene            4052..>4251
                     /locus_tag="VIN75_19310"
     CDS             4052..>4251
                     /locus_tag="VIN75_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8976373.1"
                     /translation="MPPPLTKTDFEALSEFRYQIRRFERFSEDVVQAEGITPLQYLLL
                     LHIKGYPGRNFATVGELADRLQA"
BASE COUNT          710 a         1473 c         1420 g          648 t
ORIGIN      
        1 gtccagggcc gcgtggccta cgcgaacgtc accgacgcga tggtgcctgc agcgctgggc
       61 gacacggtgt tgggcgtcgt cgggctgcag accgtgcacc tggcgcgcgt gcatgccaag
      121 cgcgccgatg cgacgcacgt cgcggcctcg tcgacgcagg ccgtcacggc cgtgagcatc
      181 ccgagctttc ccgtgatcta cggtgcgtcc gacctgccca gcgcgaccag cgccaccatc
      241 ggcatcatca cgtcgggctc cctgacgcag acgatcaagg acctcaagag cttcgcgacg
      301 agcgcgggct acccgtcccc gccggtgacg accaccgtgg tcggcagcgc gagcagcggc
      361 acctcgggca tcgacgaatg gaacatggac tcgcaggccg cgctggccgc ggccggcggc
      421 tcgatcaagt cgctcgtcct ctacaacgtc ggctcgctga gcgactcgga cctgacccag
      481 ggctacaaca gggccgtcac cgacaacacg gcccgcgcga tcaacgtctc gctcggcgcc
      541 tgcgagaccg acgaggtctc gatcgagccc acgcaggacc agatcttcga gacggcgctc
      601 acgcaaggcc agatgttctc ggtctcgtcg ggcgactccg gcgccttcga atgcggcacc
      661 gcggccggca aggcgcagag ctatcccgcg gtgtcgccgt acgtcatggc cgtcggcggc
      721 acgacgctgt cgacgagcaa cggcgcctgg gccggagaga ccgcgtggtc ctgcaccgat
      781 gcgtccaact gccagcaatc cggctccggc ggcgccggcg gcggcccctc gctgacggag
      841 aaggcgccga gctggcaggt cgcctccggc gtgctgggca cgtccaccat gcgcggcgtg
      901 cccgacatcg cgtacgacgc ctcgcccagc agcggcgcgc tcgtgctggt cgacggcagt
      961 ccggtgcaga tcggcggcac gtcgctggcc gcgccgttgt gggccggctt ctgggcgcgc
     1021 atccagtcga tggacggcaa cagcctgccc ttccccgcgc agacgctgta ccagggaggc
     1081 gcgtcgcatc ccgagtggtt ccatgacatc acgtcgggca accagggcta cgccgccacg
     1141 acggggtggg actacgcctc gggctggggc tcgatccagg tcgaccactt cgccaccgcg
     1201 ttctgcgagt gcacgccgag ccagctgacg ccggacttca gcttcacgat gaacggcctg
     1261 accgcgtcct tcgtcaactc gtccaccgac atcggctcga cgatcaactc gtaccgctgg
     1321 aacttcggcg acggcacgag cgacacggtc gccaacccga cgcataccta tccggccttc
     1381 gcgacgtaca cggccacgct gaccgtcacg gacacgatgg gcaacaccca gagcaagacc
     1441 gagggcgtgc ccgtggtgca gccgccgccc gtgcagccga tcctggccaa cggcggcttc
     1501 gagtccggca gcacgggttg gacggcgacg tcgggcgtga tctgcgcctc gaccgcttgc
     1561 gccggcgagt ccgcgcacgg cggcgccggg ttcgcctggc tggatggcta cggctcggct
     1621 cacaccgaca cgctcgcgca gacggtcaag ctgccctccg gctacaagaa cgccaggatg
     1681 acgttcttcc tgcacgtcga taccgccgag gcgaccacct ccacgcagta cgacaagctg
     1741 gcggtctcgg tcacgagcgg cagcacgagc accacgatcg gcacgtactc gaacctgaac
     1801 gcggcgccgg gctatgcgcc aaggacgctg gacctcacgc cctggctcgg caagacggtc
     1861 acgatccgtt tcagcggcac ggaggactcg tcgcgccaga cgtcgttcgt gctggacgac
     1921 atcgccatca cgctgcagtg acccggccgc gacaggccgg ccgagctggc ttcagcggcc
     1981 ggccttgttc aaccggcctg gccggccgcc acctgggtcg cgggccgctt gtcgccgcgc
     2041 agccacttcc tggcgtagcg atccttgaag tggcgcgacg cccaggaggt gtccgccacg
     2101 gcgacggtcg ccggcgcgtc ggactcggtg gcgcgcaccg ccgcgatcac gtgcgagagg
     2161 tcgcgcggat ttgacggtgc gatgtgcagc cgcggcgccg agaggttctt cgcctgcagc
     2221 gtctcgcccg gatcgagttc ctgcgacatc gcgccgaagg cgctggcggc gagcagggtg
     2281 gcggcgagga tctcgtggac ttgcatgatg aggcctgcgt gtgggtcggg ttggactcgt
     2341 ccagcggcac ggttcgggtg ccggccgggc gccgcgagcg agggtttcgc tcgtcggtac
     2401 cggcatctgc cggcgcgccc cgcgaccaca agcgggcaat cgcgcaatgg agtcttcgcg
     2461 tttcaggaat gatccgccgc cgggtcactg ccgaaggcgc gccgccgctc gagccgcgcc
     2521 gccagtgccg gcgcgccccc cagccacggc agctcagtgc cagttgccca ggcgcaggtc
     2581 gttcggcgcg ggctggccct tgcccgtctc gacgaagtcg cgcaggctga ccaggaacgt
     2641 cgcccacttg gtgctgcagt gggccatgaa ctcgacctcc tcgcgccagc cgcggtggct
     2701 gaacagcacg atcgtgaagt cgtcctggcg cgacaggctg aactcgatgg cggtgccgat
     2761 ccattcgggc ggaccgtcct tgacctgcca gcgcaccttg tcgccgggcg acagctcctt
     2821 gacgtccatg tcgaagccgc cgagcagctc gccgttctcc agatggaaca cgaacttgac
     2881 gacgccgccg accttggact cgccggtggt ctcctccgtc caccagcccg cgaggccggg
     2941 aatggtcgcg agcgcgtcgt agacccgggc ggcagggcca cggatgccga tgcgatggat
     3001 gatgtccttc atgctgaact cctcgtgctt ggttgggcgc gacgtccggg gtggcgcgtc
     3061 gacggagtcc atggtggcga cgcggcgccg cgagcgtgag taacaaggtt ggcgcgaatc
     3121 ccggcggacc atgtcgaccg aatatctccg gcgcgaccga ggtagagtcg gcgccattcg
     3181 aacaaccgaa tcgcgggagt ttccagatga gccaagccaa tccgtccgtc gacccctggt
     3241 tcgcgaaggc caggaaatgg caggtggaga tcgccagact gcgcgagatc ctcctgggtt
     3301 gcgatctggg cgaggaattg aaatggggca agccgaccta taccgcggac ggcgccaata
     3361 tcgtcctgat catgcccctg aaggacacct gcacgctgct gttcatgaag ggcgcgctga
     3421 tgaaggacgc caagggcctg ctcgtgcagc ccggcgagaa ctcgcagtcg gcgcgccaga
     3481 tgcgattcac gtcggcggcc gagatcacga aactcaaggc gacgatccgc agctacgtca
     3541 aggaggccgt cgaactggag aagtccggcg ccaaggtgga tttcaagaag agctcggacc
     3601 tgatctatcc gaaggaattc caggacaggc tcgacaggaa taccgcgctg cgcgaagcat
     3661 tcttcgcatt gacgccgggg cgccagcgcc aataccacct gcatttcacc ggcgccaagc
     3721 agtcggccac gcgggaatcg cgagtcgaga aggccattcc cctgatcctc gacgggatgg
     3781 gattgaacga tcagtgacgg tccgggaagg acgccggcgg ctggctcact ccatgagcct
     3841 ggctcgccct acagtccctt ccagatcgca gcctgatcaa gttgcagatc ccaaccctcg
     3901 ccggtttccg ccgacaggtc gacgaggact ctggcctggc cttcgcgcca ggcttttttt
     3961 tcgcgcgccg ccggcgtgac gacgagccta gttgagtaaa tacatcacgt tgtgacataa
     4021 tggcgggctt cgcccagtca cgacgagacc catgccgccg ccgctcacca agaccgattt
     4081 cgaggcgctg tccgaattcc ggtaccagat ccggcgcttc gagcgcttct cggaggacgt
     4141 cgtccaggcc gagggcatca cgccgctgca gtacctgctg ctgctgcaca tcaagggcta
     4201 cccgggccgc aacttcgcca ccgtcggcga gctggccgac cgcctgcagg c
//
LOCUS       DATCRO010000714         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_210670, whole genome shotgun sequence.
ACCESSION   DATCRO010000714 DATCRO010000000
VERSION     DATCRO010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210670"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VIN75_19315"
     CDS             <1..1259
                     /locus_tag="VIN75_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HEY8976374.1"
                     /translation="PTPPPPTPTPPPPTPTPPPPAPTPSPAPSPSSSAGTISFHLLLG
                     VSGYGNPQDSLALAGDNYTDLVMSNLLAGVMVGHLVQRYTPGQQFEKDYLYGSVLGQL
                     LQENLATQYYTAGSDLIDPNPNQQAVMGTGQGGPYQINNYAVDMVGGTYAPAGHSLIN
                     FVALQPNIGYTMANASTQYQRPTPASFNDKYYGPMLAAYFHFNDYVALNQIGKGAGGW
                     TTPWQPQYDAAIANFTHLPGNFLEMLLNAAYNQGYYGGLVAHYSALGATATASTVASV
                     DAWSNVWGDSNTYDQYPYQVRYYLNQFYDLPVPTTSATTLVTPSNHLAFPMATLRTVF
                     SNTMQKMAYAPNATTYRLFTAAQAQAAFSAALSANGVAATATLDLANASDRARIFAVL
                     ESAIGQIESITGSHFDATTLSQLSPG"
     gene            complement(1318..2550)
                     /locus_tag="VIN75_19320"
     CDS             complement(1318..2550)
                     /locus_tag="VIN75_19320"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976375.1"
                     /translation="MHGSSRYAALAVSPDTHAFAWGDNGAGMLDQDAAPSSLTSLDVA
                     TPTLILPGMQFTGALQATMGERSGALLLSDGALYAWGDNGSRQLGADADSQPVLLPIN
                     VFDLTGKPSLVNAVQAEIGDANGVALMHDGGVISWGNFAGDGSTANDRLPAQVVAPGG
                     GAPLTHAVAVSAGWNYSLALTDDGKVLAWGFESNTGALGSGTVISGSVVTPGYVLRAD
                     GTPLTGIVQVSAGYDFSLALAADGTVWAWGDNAYGELGQPTIGGASAVAVQVQGPGVG
                     GKLDRIAMVAAGGHHALALDLDGHVLAWGYSPDGALGDGANKPVVNQTSVPRAVVDES
                     GSISGLSGIASIAAGYADSYALGTDGRVLSWGSNFHGALGRPSAASTDNTPARVMTAG
                     GVLTLSPDAYPNPLRHAR"
     gene            complement(2656..3057)
                     /locus_tag="VIN75_19325"
     CDS             complement(2656..3057)
                     /locus_tag="VIN75_19325"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8976376.1"
                     /translation="MQRLFRFPSAQRRDPAVEAWLWTHGGETGVMARRWFERLRVCGD
                     DVREVMHDGQPTACVGDAAFAYVDAFRVHISVGFFHGNELPDPAGLLQGSGRFMRHVK
                     LAPGRESDAAALGALIQAAYADMHARLERAR"
     gene            complement(3086..>3427)
                     /locus_tag="VIN75_19330"
     CDS             complement(3086..>3427)
                     /locus_tag="VIN75_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8976377.1"
                     /translation="FGYVNKGGDPQQLVAAVRTVSQGKKFVTPDIAQMLVESLTAPEV
                     AHAHEKLSDRELQTLVMIASGKRLSDIASELTLSPKTVSVYRARVLEKLGLTNNSELT
                     VYAIRNGLVGE"
BASE COUNT          513 a         1288 c         1152 g          474 t
ORIGIN      
        1 cgcccacccc gccgccgccg acgccgactc ccccgccgcc caccccgaca ccgccgccgc
       61 ccgcaccgac gccgtcgccc gccccgtcgc cctcgtcctc cgcgggcacc atcagcttcc
      121 acctgctgct gggcgtcagc ggctacggca acccccagga cagcctcgcg ctggccggcg
      181 acaactacac cgacctcgtc atgtccaacc tgctcgcggg cgtgatggtc ggccacctgg
      241 tccagcgcta cacgccgggc cagcagttcg agaaggacta tctctacggc tcggtgctgg
      301 gccagctgct gcaggagaac ctggcgacgc agtactacac cgccggcagc gacctcatcg
      361 acccgaaccc gaaccagcag gcggtgatgg gcacgggcca gggcggcccg taccagatca
      421 acaactacgc ggtcgacatg gtgggcggca cgtacgcgcc cgccggccac tcgctgatca
      481 acttcgtcgc gttgcagccc aacatcggct acacgatggc caacgcctcg acgcagtacc
      541 agcgcccgac gcccgcctcg ttcaatgaca agtactacgg cccgatgctg gcggcgtact
      601 tccatttcaa cgactacgtc gcgctgaacc agatcggcaa gggcgccggc ggctggacga
      661 cgccctggca gccgcagtac gacgccgcca tcgcgaattt cacgcacctg ccgggcaact
      721 tcctggagat gctgctcaac gccgcctaca accagggcta ttacggcggg ctcgtggcgc
      781 actacagcgc gctgggggcc accgccacgg cctcgaccgt cgcctcggtc gacgcgtgga
      841 gcaacgtctg gggcgactcc aatacgtacg accagtaccc gtaccaggtg cgctactacc
      901 tgaaccagtt ctacgacctg cccgtgccca cgacgtcggc caccacgctg gtgacgccgt
      961 ccaaccacct ggcgttcccg atggccacgc tcaggaccgt cttcagcaac acgatgcaga
     1021 agatggcgta cgccccgaac gcgacgacct accggctgtt caccgccgcc caggcgcagg
     1081 ccgcgttcag cgccgcgctg tccgccaacg gcgtcgccgc gaccgcgacg ctggacctgg
     1141 ccaacgcgtc cgaccgcgcg cgcatcttcg ccgtgctgga gtcggcgatc gggcagatcg
     1201 agtcgatcac gggctcgcat ttcgatgcga ccacgctgtc gcagctgtcg ccgggttgag
     1261 gcccgaaagc aggacacccg cgtcgtgcgg gcgtcctgcc tggcgtgggc cacgggctca
     1321 gcgcgcgtgc cgcagcgggt tgggataggc gtcgggcgac agtgtgagca cgccgcccgc
     1381 ggtcatgacg cgcgccggcg tgttgtccgt gctggccgcc gacgggcggc ccagcgcgcc
     1441 gtggaagttg ctgccccagc tcaggacgcg gccgtcggtg cccagcgcgt agctgtcggc
     1501 gtagccggcc gcgatcgacg cgatgccgga caggccgctg atgctgcccg attcgtcgac
     1561 caccgcgcgc ggcaccgagg tctggttgac cactggcttg ttggcgccgt cgccgagcgc
     1621 gccgtcgggt gagtagcccc atgccaggac gtggccgtcc agatcgagcg ccagcgcatg
     1681 atggccgccg gcagccacca tcgcgatgcg atcgagcttg ccgccgacgc ccggcccctg
     1741 cacctgcacg gcgacggcac tggcgccccc gatcgtcggc tggcccagtt cgccataggc
     1801 gttgtcgccc caggcccaga ccgtgccgtc ggcggccagc gcgaggctga agtcgtagcc
     1861 ggccgacacc tgcacgatgc cggtcaacgg cgtgccgtcc gcgcgcagga cgtagccagg
     1921 cgtcacgacg ctgccgctga tgacggtgcc gctgcccagc gcgccggtgt tggactcgaa
     1981 gccccacgcc agcaccttgc cgtcgtcggt cagcgcgagc gagtagttcc acccggccga
     2041 cacggccacg gcatgcgtga gcggcgcgcc gccgccgggc gcgaccacct gcgccggcaa
     2101 ccgatcgttg gcggtcgaac cgtcgccggc gaagttgccc cagctgatca ccccgccgtc
     2161 atgcatcagg gcgacgccgt tcgcgtcgcc gatctccgcc tggacggcgt tgacgagcga
     2221 gggcttgccg gtcaggtcga agacgttgat cggcaacagc acgggctggc tgtccgcatc
     2281 ggcgccgagc tggcgcgaac cgttgtcgcc ccaggcgtac agcgcaccgt cggacagcag
     2341 gagcgcgccc gaacgctcgc ccatcgtcgc ctgcagcgcg ccggtgaact gcatgccggg
     2401 caggatcagc gtgggcgtcg ccacgtcgag cgacgtcagc gagctcggtg cggcatcctg
     2461 gtccagcatg ccggcgccgt tgtcgcccca ggcgaaggcg tgcgtgtcgg ggctcaccgc
     2521 gagcgcggcg tagcgcgacg acccgtgcac cccgctccag tggcgctggg gtacgccggt
     2581 gatcgacagc ggcgccgtga cgccgccgcg atctcgcggg aaccttgggg attgaccgcg
     2641 gcgggcggcc gccgctcagc gtgcgcgctc aaggcgcgcg tgcatgtcgg cgtacgccgc
     2701 ctggatcagc gcaccgagcg ccgcggcgtc ggactcgcgt ccaggcgcca gcttgacgtg
     2761 gcgcatgaag cggccgctgc cctgcagcag gccggccgga tcgggcagct cgttgccgtg
     2821 gaagaagccg acgctgatgt gcacccggaa cgcgtcgaca taggcgaagg ccgcgtcgcc
     2881 gacgcaggcg gtcggctggc cgtcgtgcat cacctcgcgg acgtcgtcgc cgcacacgcg
     2941 caggcgttcg aaccagcgcc gggccatcac gcccgtctcg ccgccgtgcg tccacagcca
     3001 ggcctcgacc gcagggtcgc gccgctgggc ggacgggaaa cggaacaagc gctgcatcgc
     3061 cgacctcgcc ggagcgcgcg cggcgctact cgccgaccag cccgttgcgg atcgcgtaga
     3121 ccgtcagctc ggagttgttg gtcagcccca gcttctccag cacccgcgcc cggtacacgc
     3181 tgaccgtctt gggcgacagc gtcagctcgc tggcgatgtc cgacagccgc ttgcccgagg
     3241 cgatcatcac gagcgtctgc agctcgcggt cggacagctt ctcgtgcgcg tgcgccacct
     3301 cgggcgcggt caggctctcg acgagcatct gcgcgatgtc gggcgtgacg aacttcttgc
     3361 cctgcgacac ggtgcgcacc gcggccacca gctgctgcgg gtcgccgccc ttgttgacgt
     3421 agccgaa
//
LOCUS       DATCRO010000715         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_53184, whole genome shotgun sequence.
ACCESSION   DATCRO010000715 DATCRO010000000
VERSION     DATCRO010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53184"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VIN75_19335"
     CDS             complement(<1..611)
                     /locus_tag="VIN75_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8976378.1"
                     /translation="MSEIIDHLASLTGLRDRDTLDVSLAHAMRDFLAPRRVAVYRLVG
                     DQGEERWLTRAALLADSPAATADPAWVDLDSLPRATERPDWLECLTTAKIIEVRAEGL
                     FTTLFPVMSDTGAVGVIELQTTRGIGTKSRRLVFGVLRIYRNVQTLLDYSERDTLTGL
                     LNRKIFDDSFYKVASLPLLDAKLDEGERRHASGSRYWLGVVDI"
     gene            complement(717..>3347)
                     /locus_tag="VIN75_19340"
     CDS             complement(717..>3347)
                     /locus_tag="VIN75_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976379.1"
                     /translation="PAEPAHPAAIPVSGLGAVKFTALDDTIRPEAGERHEVAASETDA
                     GGFAQDLDAHDAIDPELFEIFSEEAQELLPNLASHLRAWEESPEMTSEAFGALRALHT
                     LKGSARLAGAMRLGELAHRLESAFEERVGGRSANPPTMAMLHTGVDALDEEFQRLLKN
                     PQGMPAPAPAPVAAPAPAPVAAPAVAEPVAAAPVAAAPVAAQPVPVAEAPVAPQAAEP
                     AAVAPESTVRQMVPKALADATPSVIDWARFSVENPSRVQRVVEAATGNPATATVRVKA
                     PLLDRLVNQAGEVSITRSRLETDVTQMRSSLRELTENLDRLRRQLRDIELQADTQIAS
                     RLEASKALSAEFDPLEMDRFTRFQELTRMMAESVNDVATVQRSITQTVQSAEDEMAAQ
                     ARLTRALSEDLLRARMVPFDALSERLYRVVRQAAKETGVQVRLNVEGSSIELDRSVLD
                     RMAGAFEHLLRNSVVHGIESPDVRRAQDKDPVGTIDVALAQQGNEVRITIRDDGAGLD
                     LQRIEARGRANGLIAADATPTEAELANLIFTPGFSTAESVTELAGRGVGMDVVRSDIT
                     ALGGRIETASAPGRGTAFTLLLPLTTAVTRVVQLRCGDLTVAMPASLVEVVRRTTNAD
                     IEQAYKSGVYQDGTNEVPFFWLGALLQSSTHGVLGGKTNSVVLVRSAEQRVALHVDQV
                     VGNQEVVVKNLGPQLARLPGLAGMSLLATGEVVPIYNPVALAAVYGARPTTEAGAPHV
                     PVVVEEVEEAKAPLILVVDDSLTVRRVTQRLLTREGFRVTVAKDGLDALEQLEVERPA
                     VMLTDIEMPRMDGFDLLRNVRHDPRWAGLPVIMITSRIAQKHRDLAIQLGADHYLGKP
                     YDEEQLIGLIQENVAKTMPA"
BASE COUNT          461 a         1145 c         1226 g          515 t
ORIGIN      
        1 tcgatgtcga cgacgcccag ccagtagcgc gatcccgagg cgtggcggcg ctcgccctcg
       61 tcgagcttgg cgtccagcag cggcagcgac gccaccttgt agaagctgtc gtcgaagatc
      121 ttgcggttga gcaggcccgt cagggtgtca cgctcgctgt agtcgagcag cgtctgcacg
      181 ttgcggtaga tgcgcagcac gccgaacacg aggcggcgcg acttggtgcc gatgccgcgc
      241 gtggtctgca gctcgatgac gcccacggcg ccggtgtccg acatcaccgg gaacagcgtc
      301 gtgaagagtc cttcggcgcg cacctcgatg atcttggccg tggtcaggca ctcgagccag
      361 tcggggcgct cggtggcgcg cggcagcgag tcgaggtcga cccaggccgg gtcggcggtg
      421 gccgccggcg aatcggccag cagcgcggcc cgcgtgagcc agcgctcctc gccctggtca
      481 cccaccagcc ggtagacggc cacgcgcctg ggcgcgagga agtcgcgcat ggcgtgcgcc
      541 aggctgacgt cgagcgtgtc gcggtcgcga agcccggtca acgaggcgag gtgatcgatg
      601 atttccgaca tggatttctg aagcggtgat ccctgttatc ggacgagtgt ctcgccgact
      661 gaaaccccga gcctggggag ggttccggca aaagtgccgg aattcttcac ggattgtcag
      721 gcaggcatcg tcttcgcgac attctcctgg atcagaccga tcagctgttc ttcgtcgtag
      781 ggcttgccaa ggtagtggtc ggcgcccagc tggatggcca ggtcgcggtg cttctgggcg
      841 atgcgcgacg tgatcatgat gaccggcagg ccggcccagc gcggatcgtg gcgcacgttg
      901 cgcagcaggt cgaagccgtc catgcgcggc atctcgatgt cggtcagcat gacggccgga
      961 cgctcgacct cgagctgctc caacgcatcc aggccgtcct tggccacggt gacgcggaag
     1021 ccttcgcggg tcagcaggcg ctgcgtgacg cggcgcaccg tcagcgaatc gtccaccacc
     1081 aggatcagcg gggccttggc ctcctcgacc tcttccacca ccaccggcac gtgcggcgcg
     1141 ccggcctcgg tcgtcggacg cgcaccgtac acggccgcga gcgccaccgg gttgtagatg
     1201 ggcaccacct cgccggtggc cagcagggac atgcccgcca ggcccggcag gcgcgccagc
     1261 tgcggcccca ggttcttgac gaccacttcc tggttgccca cgacctggtc gacgtgcagc
     1321 gcgacgcgct gctcggcgct tcgcaccagc acgaccgagt tcgtcttgcc gcccagcacg
     1381 ccgtgcgtgc tcgactgcag cagggcaccc agccagaaga acggcacttc gttcgttccg
     1441 tcctggtaga cgccggactt gtaggcctgc tcgatgtcgg cgttggtggt gcggcgcacc
     1501 acttccacca gcgaggccgg catggcgacg gtcaggtcgc cgcagcgcag ctgcacgacg
     1561 cgcgtcacgg ccgtggtcag cggcagcagc agcgtgaagg cggtgccgcg gcccggggcc
     1621 gaggcagtct cgatgcggcc gccgagcgcg gtgatgtcgg aacggacgac gtccatgccg
     1681 acgccgcggc cggccagctc ggtcacgctc tcggccgtgg agaagcccgg cgtgaagatg
     1741 aggttggcga gttcggcctc ggtgggcgtc gcgtccgcgg cgatgaggcc gttggcgcgg
     1801 ccgcgcgcct cgatgcgctg caggtcgagg ccggcgccgt cgtcacggat cgtgatgcgc
     1861 acctcgttgc cctgctgggc cagcgccacg tcgatcgtgc cgaccggatc cttgtcctgc
     1921 gcgcggcgga cgtcgggcga ctcgatgccg tgcacgacgg agttgcgcag caggtgctcg
     1981 aaggcgccgg ccatgcgatc cagcaccgaa cgatccagct cgatggagga accctcgacg
     2041 ttcaggcgca cctggacgcc ggtttccttg gcggcctggc gcacgacgcg gtacaggcgc
     2101 tcggacaggg catcgaacgg caccatgcgc gcgcgcagca ggtcttccga cagcgcgcgc
     2161 gtcaggcgcg cctgcgcggc catctcgtct tccgccgact gcacggtctg cgtgatgctt
     2221 cgctgcacgg tggccacgtc gttgaccgac tcggccatca tgcgcgtgag ttcctggaag
     2281 cgcgtgaagc ggtccatctc cagcggatcg aattccgcgg acaaggcctt gctggcttcc
     2341 agtcgcgagg cgatctgcgt gtcggcctgg agctcgatgt cgcgcagctg gcgacgcagg
     2401 cgatccaggt tttccgtcag ttcgcgcagc gacgagcgca tctgggtgac gtccgtctcg
     2461 aggcgcgaac gcgtgatcga gacttcgccg gcctggttga ccaggcggtc cagcagcggc
     2521 gccttgacgc gcacggtggc ggtggccggg ttgccggtgg cggcctcgac cacgcgctgc
     2581 acgcgcgacg ggttctcgac gctgaagcgg gcccagtcga tgacgctcgg cgtcgcatcg
     2641 gccagcgcct tgggcaccat ctggcgcacc gtggactccg gcgccacggc ggccggctcg
     2701 gcggcctgcg gcgcgacggg cgcctcggcg accgggacgg gctgcgcggc aaccggcgcc
     2761 gcagcgaccg gcgcggcggc gacgggttcg gccacggccg gcgcggcgac cggcgccggc
     2821 gcgggtgcgg ccaccggcgc gggggccggc gctggcatgc cctgcgggtt cttgagcagg
     2881 cgctggaatt cctcgtcgag cgcgtcgacg ccggtgtgca gcatcgccat cgtcggcggg
     2941 ttggccgagc ggccgcccac gcgctcctcg aacgccgatt ccaggcggtg cgcgagttcg
     3001 cccaggcgca tcgcgccggc caggcgggcg ctgcccttga gcgtgtgcag ggcgcgcagc
     3061 gcgccgaagg cctcggaggt catctccggc gactcttccc aggcccgcag gtgcgacgcc
     3121 aggttcggca gcagttcctg ggcttcttcc gagaagatct cgaacagctc gggatcgatc
     3181 gcgtcgtggg cgtccaggtc ctgcgcgaag ccgccggcgt ccgtctccga cgcggccacc
     3241 tcgtggcgct cgccggcttc ggggcggatg gtgtcgtcca gcgccgtgaa cttgaccgcg
     3301 cccaggcccg agaccgggat cgcggcggga tgggccggct cggcggg
//
LOCUS       DATCRO010000716         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_97935, whole genome shotgun sequence.
ACCESSION   DATCRO010000716 DATCRO010000000
VERSION     DATCRO010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97935"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..3234
                     /locus_tag="VIN75_19345"
     CDS             439..3234
                     /locus_tag="VIN75_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976380.1"
                     /translation="MFQRTKICTGLLMAFGSGLLTVAPGAFAQDTTVQRVEITGSSIK
                     RVDAETSVPVTVITADELKKAGVTSVEQVLTQVSSVQVQLTSAQAVGAGTGGSSYADM
                     RGLGYNKTLVLLNGRRLANSAFTSSAPDINTIPFAAIERVEVLRDGASALYGTDAIAG
                     VINVITRKDYRGGTVTLGVDSPQHPGGAQHEAQVGFGFGDLEKDGLNVFGFVGFQKQA
                     AIGASQRHLAPHKTTSGTTFPATSYLTDGTVDSDGNLNVTAYTPFAGPTGCKDPTLFP
                     SGDGTSCGEDTNRFVDYTPATSRVSGLLNAAFKINQNNTFNLEAFVAESKVNSRIAPV
                     PYAPVFLDPTSPYYPGNGITPLPTGLTLGPDQVDATPGAFYVNNGTGTANQGRIGTRF
                     RDVINGFREDDNTTTQARFTASLDGTLGDWDYTLAFALNTTHTQDMLAHGYSDENVLA
                     TLDTDPNSATQGYYVLNNLINPYGAQTPAGLALLKSAQKIGVLQYGNGTVKDVDGHAS
                     RDLGDWLHAGRPAAIAIGGEFRDEDFLNQANAGYAAQVIASTGVDPATYNAGTRKVYA
                     GYVELNVPVLKSLDVTAALRYDHYSDFGSTTNPKVSFRFQPAKAFLLRGSFSTGFRAP
                     SLYELNAAQTFTNSGAGVSDPVNCPNGVPLNGHQLSDVCSTDATNIQFVDKQGGNPNL
                     KAEKSKNSTLGLVLEPFNNFTTEFDVYDIRITKEVGVLPDDLLYSPKGYAEFPGNFHY
                     NSSGLLSQTPQNCPGPACGWVDTLNQNIGAVHTNGIDVAMGYKANAGSLGRFNFELQS
                     TWVHSYKYQLVPGGAYTENVGIFSKGNGEPVFRWQHNATIDWNLAPFSLGFAVHHKTG
                     YQDFTPNRRVSAYTTEDLYATYAMPKGFSFTVGVKNLADRLPPYTNYNKLFQQGYDPR
                     YYDPTGRTYYARATYSF"
     gene            complement(3465..4223)
                     /locus_tag="VIN75_19350"
     CDS             complement(3465..4223)
                     /locus_tag="VIN75_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY8976381.1"
                     /translation="MEISGKVFIVTGAASGLGEGTARMLVAHGAKVVLADLQEERGQA
                     IAAELGQVFVRCDVTQEADGRRVVDAAKALGKLMGLVNCAGIAPASRTVGKTGAHALD
                     LFAKVITINLIGTFNMIRLAAEAMSANEPESTGERGVLISTASVAAYDGQIGQAAYSA
                     SKGGVVGMTLPIARDLAKTGIRNMTIAPGIFGTPMLFGMPQDVQDALAANVPFPSRLG
                     TPADFAKLVHQIVTNEMLNGEVIRLDGAIRLAPR"
     gene            complement(4406..5062)
                     /gene="adk"
                     /locus_tag="VIN75_19355"
     CDS             complement(4406..5062)
                     /gene="adk"
                     /locus_tag="VIN75_19355"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8976382.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVKAGTP
                     LGLAAKQVMDAGGLVSDDLIISLVKERIAQADCAKGFLFDGFPRTIPQADAMKAAGVK
                     LDLVLEIDVPDAAIVERMSGRRVHVASGRTYHIHNNPPKVEGKDDVTGEPLTQRPDDA
                     EDIVRKRLATYHEQTEPLVAYYEKWAATGDAQAPRCRKISGTGSVDEITKRALQALEN
                     "
     gene            complement(5258..>5863)
                     /gene="kdsB"
                     /locus_tag="VIN75_19360"
     CDS             complement(5258..>5863)
                     /gene="kdsB"
                     /locus_tag="VIN75_19360"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY8976383.1"
                     /translation="RQHGVEAVLTRVDHASGSDRLAEACTLLGLDGTDIVVNVQGDEP
                     LIDPALIRQCAGLLETDGEAVVATAAHAIDDAADFTNPNVVKVVLDAKFRALYFSRAP
                     LPWWRDASTAGHPALPKTGAHDVGALRHIGLYAYRAGYLRTFPTLAMAPLERLESLEQ
                     LRVLWHGERIVVHIAPQAPPGGVDTAEDLARVRALLATAND"
BASE COUNT          963 a         1967 c         1911 g         1022 t
ORIGIN      
        1 cgctcatcga cgccgtgccg cgcctggacc tgccggtctg accagtcctg tctagggaac
       61 cacccgcggc ccccgggagg gcggggcggg ccgggggtat cggcgaaacc gggaaaaccg
      121 ggttattcgc acgccgcggg agggtttgta cgcacggacg cgcgccgccg tgttacgtgg
      181 ttattccctc gtgtgcccgg ccgggcaaac ggtgttgctc atgcgtcgga aagattctgg
      241 attcgctgca ttctcgcaac agtttgctta gggaaaacgt tcggaacacg tttcagttgg
      301 tgagtcgggt acgccgttca tggacaatct tttgacaagc tttattggcg ggtcatcagt
      361 cgttcacaaa ccggcacatg acgcgcttgt caggctgggt ctatcgatca acctttccac
      421 cccctcagga gggaacccat gtttcaacgt acgaagatct gtaccggtct tctcatggct
      481 ttcggcagcg gcctgctgac cgtcgccccg ggcgcttttg ctcaggacac caccgttcaa
      541 cgcgtggaaa tcacgggctc gtccatcaag cgcgttgacg ccgagacgtc cgtccccgtc
      601 accgtcatca ccgctgacga actgaagaag gccggtgtca ccagcgtcga gcaggttctc
      661 acccaagtca gctcggtgca ggttcagctg acctccgcgc aggcggtcgg tgccggcacc
      721 ggtggcagtt cttacgccga catgcgcggc ctgggttaca acaagaccct cgtgctgctg
      781 aatggccgtc gtctggccaa cagtgcgttc acgtcgtcgg ctcccgacat caacaccatt
      841 cccttcgccg cgatcgaacg cgtcgaagtg ctgcgtgacg gcgcttccgc gctttacggc
      901 accgacgcca tcgccggcgt gatcaacgtg atcacccgca aggattatcg cggcggcacg
      961 gtcacgctgg gcgtggattc gcctcagcac ccgggcggcg cacagcatga agcccaagtg
     1021 ggcttcggct tcggcgacct ggagaaggac ggtctgaacg tcttcggctt cgtgggcttc
     1081 cagaagcagg cggccatcgg cgccagccag cgccacctgg cgccgcacaa gacgacgtct
     1141 ggaacgacct tccccgcgac cagctatctg accgacggca cggtcgattc cgacggcaac
     1201 ctcaacgtca cggcgtacac gccgttcgcc ggcccgaccg gttgcaagga cccgacgctc
     1261 ttcccgtcgg gcgacggcac gagctgcggc gaagacacga acaggttcgt cgactacacg
     1321 ccggcgacgt cgcgcgtgtc cggcctgctc aacgcggcct tcaagatcaa ccagaacaac
     1381 accttcaacc tcgaagcgtt cgtcgccgag agcaaggtca attcgcgtat cgcgccggtg
     1441 ccttacgcgc ccgtgttcct tgatccgacc agcccgtact acccgggcaa tggcatcacg
     1501 ccgctgccca ccggcctgac gttgggtccg gaccaggtgg acgccacccc tggcgcgttc
     1561 tatgtcaaca acggcaccgg cacggccaac cagggccgta tcggcacgcg tttccgtgac
     1621 gtgatcaacg gcttccgtga agacgacaac acgaccacgc aggcgcgctt cacggcctcc
     1681 ctggacggta cgctcggcga ctgggactac accctcgcgt tcgctttgaa cacgacgcac
     1741 acgcaagaca tgctggcgca cgggtattcg gatgagaacg tcctggcaac gctggatacg
     1801 gatccgaact cggcgaccca gggttattac gtcctgaaca acctgatcaa tccgtatggt
     1861 gctcagacgc cggcgggcct ggccctgctg aagtccgcgc agaagatcgg tgtgctgcaa
     1921 tacggcaatg gcaccgtcaa ggacgtggac ggccatgcca gtcgcgacct gggcgattgg
     1981 ctgcatgctg gccgcccggc tgcgatcgct atcggtggcg agttccgcga cgaggacttc
     2041 ctgaaccagg ccaacgcggg ctatgcggcc caggtcatcg ccagcaccgg cgtcgaccct
     2101 gccacgtaca acgcgggcac gcgcaaggtc tacgccggct acgtcgagct caacgtgccc
     2161 gtgctcaaga gcctggacgt cactgccgcg ctgcgctatg accactacag cgactttggc
     2221 agcacgacga acccgaaggt cagcttccgc ttccagccgg ccaaggcctt cctgctgcgc
     2281 ggttccttct ccacgggatt ccgtgccccg tcgctgtatg aactgaacgc cgcgcagacg
     2341 ttcaccaact ccggcgccgg cgtgagtgac ccggtcaact gcccgaacgg tgttccgctg
     2401 aacggtcacc agctgagcga cgtctgcagc accgacgcta cgaacatcca gttcgtcgac
     2461 aagcagggcg gcaacccgaa cctgaaggcc gaaaagtcga agaactcgac cctgggtctg
     2521 gtgctcgagc cgttcaacaa cttcacgact gagttcgatg tctacgacat ccgcatcacg
     2581 aaggaagttg gcgtcctgcc ggacgacttg ctgtactctc cgaagggtta cgcggaattc
     2641 ccggggaact tccactacaa ctcgtcgggc ctgctgtcgc agacgccgca gaactgcccg
     2701 ggcccggcct gcggttgggt ggacacgctc aaccagaaca tcggtgccgt gcacacgaac
     2761 ggtatcgacg tggcgatggg ctacaaggcc aacgctggtt cgctcggccg ctttaacttt
     2821 gagctgcaga gcacctgggt gcattcctac aagtaccagc tggttccggg tggcgcttat
     2881 acggagaacg tcggcatctt ctccaagggc aatggcgagc cggtgttccg ctggcagcac
     2941 aatgcgacga tcgactggaa cctggctccg ttctcgctgg gctttgccgt ccatcacaag
     3001 acgggttacc aggacttcac gccgaatcgc cgcgtcagcg cttatacgac ggaagatctg
     3061 tacgccacct acgcaatgcc gaagggcttc tcgttcacgg tcggtgtcaa gaacctggcc
     3121 gaccgcctgc cgccgtatac caattacaac aagctgttcc agcaaggcta cgacccgcgt
     3181 tactacgatc cgacgggccg tacgtactac gctcgcgcga cgtactcctt ctaaggagtt
     3241 cgccgcagcc cggatgccgc cgtgcatccg gcctcttgcg cgcaagggcg cgaatcactt
     3301 ccgggtggtt cgcgcccttt tcacatgggc ggcgcgaacg cgtgcgcgca atgagaacgg
     3361 ccccaggcat gtcgcctggg gccgtcgttc atcgagtcct cggatgcgcc gcgcgcacgc
     3421 gtgcgcggcg cccgcgctcg cgctcgcgcg cggacgcgcg aggctcagcg cggtgcgagg
     3481 cggatcgcgc cgtccaggcg gatcacttcg ccattgagca tctcgttggt gacgatctgg
     3541 tgcaccagct tggcgaagtc ggcgggcgtg cccaggcggc tggggaaggg cacgttggcg
     3601 gccagcgcgt cctgcacgtc ctggggcatg ccgaacagca tcggcgtgcc gaagatgccg
     3661 ggggcgatgg tcatgttgcg gatgccggtc ttggcgaggt cgcgcgcgat cggcagcgtc
     3721 atgcccacca cgccgccctt gctggcggag taggcggcct ggccgatctg gccgtcgtag
     3781 gcggccacgc tggcggtgct gatcagcacg ccgcgctcgc ccgtcgactc gggctcgttg
     3841 gcgctcatcg cctcggccgc caggcggatc atgttgaatg tgccgatcag gttgatcgtg
     3901 atgaccttgg cgaacaggtc cagcgcgtgc gcgccggtct tgccgaccgt gcgcgacgcc
     3961 ggcgcgatgc cggcgcagtt gaccaggccc atcagcttgc cgagggcctt ggcggcgtcg
     4021 accacgcggc ggccgtcggc ctcctgcgtg acgtcgcagc gcacgaagac ctgccccagt
     4081 tcggccgcga tggcctggcc gcgctcttcc tgcaggtcgg cgagcaccac cttggcgcca
     4141 tgggccacca gcatgcgcgc cgtgccttcg cccagccccg acgccgcgcc ggtgacgatg
     4201 aataccttgc ccgagatttc catgcttgct cctgtgccgg cgtcggcgcc gcggaaggcg
     4261 cggccggcgt gattgacgtt aacgtaaacg tcaattgtcg ctcgaacggc gcgcaaggca
     4321 agaggggcgc atcgaccggg gccggaaacg aagaagcccg cgacgtcgcc ggcgcgggct
     4381 ccgagggcgc gaggcgcgcc ggctttcagt tttccagggc ctgcagggcg cgtttggtga
     4441 tctcgtcgac gctgcccgtg ccgctgatct tgcggcagcg cggcgcctgg gcgtcgcccg
     4501 tggcggccca cttctcgtag taggccacca gcggctcggt ctgctcgtgg taggtggcca
     4561 ggcgcttgcg cacgatgtcc tcggcgtcgt ccgggcgctg ggtcagcggc tcgccggtga
     4621 cgtcgtcctt gccttcgacc ttgggcggat tgttgtggat gtggtacgtg cgacccgagg
     4681 ccacgtgcac gcggcggccg ctcatgcgct cgacgatcgc ggcgtcgggc acgtcgatct
     4741 ccagcacgag gtcgagcttg acgcccgcgg ccttcatcgc gtcggcctgc gggatcgtgc
     4801 gcgggaagcc gtcgaacagg aaacccttgg cgcaatcggc ctgcgcgatg cgctccttga
     4861 ccagcgagat gatcaggtcg tcgctcacca ggccgccggc gtccatcacc tgcttggcgg
     4921 ccaaccccag cggcgtgccc gccttcaccg cggcgcgcag catgtcgccc gtggagatct
     4981 gcggaatgcc gtatttctgg cagatgaacg ccgcctgcgt tcccttgcct gcgccggggg
     5041 cgcccagaag aatcaatctc atcgcttgcc tttggagtcg gaatatcttg atggcgcgaa
     5101 cccgttatct cgtgcggacg tcgccgcgat atcgggtggc ctggagagcc gggattactc
     5161 gacagaatat catgcgcacc cttgcagggc cttactgcac cagcatggaa ctccgatggc
     5221 aaccggcggt tttgcgcgcg gcgcggagtt tgttccgtca gtcgttcgcg gtcgccagca
     5281 aggcgcgcac gcgggccaga tcttcggcgg tgtcgacgcc gccgggcggc gcctggggcg
     5341 cgatgtgcac cacgatgcgc tcgccgtgcc acagcacgcg caattgctcg agcgactcga
     5401 gccgctccag cggcgccatg gccagcgtgg ggaacgtgcg caggtagccc gcccgatagg
     5461 cgtacaggcc gatgtggcgc agcgcgccga cgtcgtgcgc gccggtcttg ggcagcgcgg
     5521 ggtggccggc cgtcgatgcg tcgcgccacc agggcagggg cgcgcggctg aagtacagcg
     5581 cgcggaactt cgcgtccagc accaccttga ccacgttcgg gttcgtgaag tcggcggcgt
     5641 cgtcgatcgc gtgcgccgcg gtggcgacca cggcctcgcc gtcggtctcg agcaggcccg
     5701 cgcactggcg gatcagcgcg ggatcgatca gcggttcgtc gccctgcacg ttgacgacga
     5761 tgtcggtgcc gtccaggccc agcaacgtgc acgcctcggc gaggcggtcg ctgccgctgg
     5821 cgtggtcgac gcgcgtcagc acggcctcga cgccatgctg gcg
//
LOCUS       DATCRO010000717         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_795935, whole genome shotgun sequence.
ACCESSION   DATCRO010000717 DATCRO010000000
VERSION     DATCRO010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795935"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VIN75_19365"
     CDS             <1..1193
                     /locus_tag="VIN75_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976384.1"
                     /translation="MSVLKALHDFLVGAMRVERRVDPFFRPLVDALTREPLTRLVQAL
                     IRARLPDDGLALAQEKPIAGEDALVASIIADMSTYLRTHYTAGTAQRGGNTKTHGVVR
                     GELVIHDGLPANLRHGLFAQPARYPAWVRFGGPGPALPPDIEDVGVLSIGVKVMGVPG
                     PKLLDDERFTQDFTGISTPVFTTPDLIANAKLQAAIGRGLPLFYFIGPGGHFLDALMQ
                     GLWSRTQTSPLETPYWSCVPYLLGEGQAMQYAFRPVAATRSRIPGLPGRPSDNYLREA
                     MARTLTAESVRLEMLLQVQTDPRRMPVEHASVRWFDAGGTPVVAATLELPRQRFDSEA
                     QLAFANRLSINPWHCLPAHRPLGNQNRGRLKIYQALSQLRQQMNATPHVEPDGSESFD
                     PPAC"
     gene            complement(1413..2915)
                     /locus_tag="VIN75_19370"
     CDS             complement(1413..2915)
                     /locus_tag="VIN75_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976385.1"
                     /translation="MSTATAFSDFADVQGLLRFGYKHHVESAFALLRVRNRAAARAWL
                     ASAPVGSAEKGAPAPVVLQIAFGAEGLRELGVPDAVCATFAPEFLGGLGTDANRARRL
                     GDLGVNDPARWLWGGPGAEQAPHVLVMVYALPGRLDAACRELESHWGIAFDLMRWLST
                     TDMGGVEPFGFADGLSQPEIDWAREIPPEDHDRLRYGNLSCLGEFLLGYPNEYGRYTA
                     RPLLEPAGAAAHLPRAEDAPDRADLGRNGSYLVLRTLKQDVPGFWRYVDAQSGGDAAQ
                     RKQLAEAMVGRRMDGQPLLLPDDAPIAGVGSDAAARAKNGFTFDGDPAGVRCPVGAHI
                     RRANPRNPDLPAGTSGLISRAIRMLGFDADVLHADAVASVRFHRVLRRGREYGVRMSV
                     SEALSGRAPQVETGLHFICLCADLARQFEFVQGAWIAGSKFAALPHEADPLLGNRLSA
                     PDEPDTARFTMPRAGCPSRVVDGMPPFIEVTGGAYFFLPGVRALRWLATA"
     gene            complement(2912..>3354)
                     /locus_tag="VIN75_19375"
     CDS             complement(2912..>3354)
                     /locus_tag="VIN75_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976386.1"
                     /translation="VVVLVALLDYAARHVYGRGHLTRVQTIHFARWVFIDDRKRLLFA
                     SNYDGILESYMDDFINKVAWGLNLVFSNGVGYPRTNWLVQGGARNSQAFKNYLRRHQV
                     ANSVWYKADAGLSAMDLARHTRIREGLERTSMNDREIEDWLRLM"
BASE COUNT          488 a         1209 c         1200 g          457 t
ORIGIN      
        1 tcatgtccgt gctgaaggcc ctgcacgatt tcctggtcgg cgcgatgcgc gtggagcgcc
       61 gcgtcgaccc gttcttccgg ccgctggtgg acgccctcac gcgcgagccg ctcacgcgcc
      121 tggtgcaggc gctgatccgg gcgcgcctgc cggacgacgg cctcgcgctg gcgcaggaaa
      181 agccgatcgc cggcgaggac gcgctggtgg ccagcatcat cgcggacatg tcgacctacc
      241 tgcgcacgca ctacacggcg ggcaccgcgc agcgcggggg caacaccaag acgcacggcg
      301 tcgtgcgcgg cgagctcgtg atccacgacg gcctgcccgc gaacctgcgt cacggcctgt
      361 tcgcgcaacc ggcgcgctat cccgcctggg tgcgcttcgg cggccccggg cccgcgctgc
      421 cgcccgacat cgaggacgtc ggcgtcctga gcatcggcgt caaggtgatg ggcgtgcccg
      481 gccccaagct gctggacgac gagcgcttca cgcaggactt caccggcatc agcacgccgg
      541 tgttcaccac gcccgacctg atcgccaacg cgaagctgca ggccgcgatc ggccgggggt
      601 tgccgctgtt ctacttcatc ggccccggcg gccacttcct cgacgcgttg atgcagggcc
      661 tgtggtcgcg cacgcagacc agcccgctgg agacgccata ctggagctgc gtgccctacc
      721 tgctgggcga gggccaggcg atgcagtacg cgttccggcc cgtggcagcc acgcgcagcc
      781 gcatcccggg cctgccgggt cggccgtcgg acaactacct gcgcgaagcg atggcgcgca
      841 cgctcaccgc ggagtccgtg cgcttggaga tgctgctgca ggtgcagacc gacccgcgcc
      901 gcatgcccgt cgaacacgcg tccgtgcgct ggttcgacgc cggcggaacg cccgtggtgg
      961 ccgccacgct ggaactgccg cggcagcgct tcgacagcga agcccagctc gcgttcgcga
     1021 accggctgtc gatcaacccg tggcactgcc tgccggcgca ccgtccgctg ggcaaccaga
     1081 accgcggccg gctgaagatc taccaggcgc tgtcgcaact gcgccagcag atgaacgcga
     1141 cgccgcacgt cgagcctgac ggcagtgaga gtttcgatcc accggcctgt tgagatcgca
     1201 cggcctttcg tcagcgcgat cacgtcattc cgcgcgcagt cgcggaatcc accgcgacct
     1261 ggaaagggca ttgttcgcca gcgtggctcc tgcgactgcg cgcaggatga cggcgtggcg
     1321 ggtcgtgacc gcatggcaaa tggcgatggc ctggcaggca acgggccggc ggttgcgacg
     1381 gcctggcggt cgtgcccgcg gtcgcagggc gctcaagcgg tggcgagcca gcgcagcgcg
     1441 cgcacgccag gcaggaaaaa gtaggcgccg ccggtcacct cgatgaacgg cggcatgccg
     1501 tccacgacgc ggctgggaca gcccgcgcgc ggcatcgtga agcgcgcggt gtcaggctcg
     1561 tcgggcgccg agaggcgatt gcccagcagc gggtcggcct cgtgcggcag cgccgcgaac
     1621 ttgctgccag cgatccaggc gccctgcacg aactcgaact ggcgcgccag gtcggcgcac
     1681 aggcagatga agtgcagccc cgtctcgacc tgcggggcgc ggcccgacag cgcctcgctc
     1741 accgacatcc gcacgccgta ctcgcgcccg cgccgcagca cgcggtggaa gcgcaccgag
     1801 gcgacggcgt cggcatgcag cacatcggca tcgaagccca gcatgcggat cgcgcgcgag
     1861 atcagccccg acgttccggc cggcaggtcg ggattgcgcg ggttggcgcg acggatgtgc
     1921 gcgcccacgg ggcagcgcac gcccgccgga tcgccgtcga acgtgaagcc gttcttcgcg
     1981 cgcgcggcgg cgtcgctgcc cacgcccgcg atgggtgcgt cgtccggcag cagcagcggc
     2041 tggccgtcca tgcggcggcc gaccatcgcc tcggccagct gcttgcgctg cgccgcatcg
     2101 ccgccggact gcgcatcgac gtagcgccag aaacccggca cgtcctgctt caaggtgcgc
     2161 agcaccaggt agctgccgtt gcgtccgagg tcggcgcggt cgggcgcgtc ctcggcgcgc
     2221 ggcaggtgcg cggcggcgcc cgcgggctcc agcagcgggc gcgcggtgta acggccgtac
     2281 tcgttggggt atccgagcag gaactcgccc aggcaggaca ggttgccgta cctgagccgg
     2341 tcgtggtcct cgggcgggat ctcgcgcgcc cagtcgatct cgggctggct caggccgtcc
     2401 gcgaagccga agggctcgac gccgcccatg tcggtcgtcg acagccagcg catcaggtcg
     2461 aacgcgatgc cccagtggct ctcgagctcg cggcaggcgg cgtcgagccg gccgggcagc
     2521 gcgtagacca tcaccagcac gtgcggcgcc tgctcggccc ccgggccgcc ccacagccag
     2581 cgcgcggggt cgttgacgcc gagatcgccg agccggcgcg cgcggttcgc gtcggtgccc
     2641 aggccgccga ggaattcggg ggcgaacgtg gcgcacacgg cgtccggcac gcccagctcg
     2701 cgcaggccct cggcgccgaa cgcgatctgc agcacgacgg gcgccggcgc gcccttttcc
     2761 gccgagccca ccggcgcgct ggccagccat gcgcgggcgg cggcgcggtt gcgcacgcgc
     2821 agcaacgcga aggcgctctc gacgtggtgc ttgtagccga agcgcagcag gccctgcacg
     2881 tcggcgaagt ccgagaaggc ggtcgcggtg ctcacatcag cctcagccag tcctcgatct
     2941 cgcgatcgtt catggaggtg cgctccagcc cctcgcggat gcgcgtgtgg cgcgcgagat
     3001 ccatggcgct gaggcccgcg tcggccttgt accagaccga gttggccacc tggtggcgcc
     3061 gcaggtagtt cttgaacgcc tgcgagttgc gcgcgccgcc ctgcaccagc cagttggtgc
     3121 gcggataccc cacgccgttg ctgaacacga ggttcaggcc ccaggcgacc ttgttgatga
     3181 agtcgtccat gtagctctcc aggatgccgt cgtagttgct ggcgaagagc aggcgcttcc
     3241 tgtcgtcgat gaacacccag cgcgcgaagt ggatcgtctg cacgcgcgtg aggtggccgc
     3301 ggccgtagac gtggcgggcc gcgtagtcca gcagcgccac cagcaccacc acca
//
LOCUS       DATCRO010000718         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_863485, whole genome shotgun sequence.
ACCESSION   DATCRO010000718 DATCRO010000000
VERSION     DATCRO010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863485"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VIN75_19380"
     CDS             complement(<1..301)
                     /locus_tag="VIN75_19380"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552983.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HEY8976387.1"
                     /translation="MSTLSDRMSRFLRADVQGMHGYAVQPSAGFVKVDTMENPFRLPD
                     ALRKELGERLAEVALNRYPAERGDVLRAELAKHANMPADCDIMLGNGSDELISLLT"
     gene            complement(298..768)
                     /locus_tag="VIN75_19385"
     CDS             complement(298..768)
                     /locus_tag="VIN75_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8976388.1"
                     /translation="MSAVPPVASPRIFVDTGVLLHAVDDHDPERRDRARAWLAGCWTA
                     RCGRTSVQVLSEFYAHARRHFPTAISAGDARALVRRYQHWKPWVVDHATIETAWAIES
                     RHELPYADALILAAAQQQECRFVASDRWTHGRQIESLTVVDPFASSFDTLGAAA"
     gene            complement(765..983)
                     /locus_tag="VIN75_19390"
     CDS             complement(765..983)
                     /locus_tag="VIN75_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY8976389.1"
                     /translation="MKNVTVTMEDQVADWARMEAARRNTSVSRLIGELLAEKMRHDDS
                     YERAMREALEFKSFGRSDGRPFERGMSR"
     gene            complement(1217..2536)
                     /gene="hisD"
                     /locus_tag="VIN75_19395"
     CDS             complement(1217..2536)
                     /gene="hisD"
                     /locus_tag="VIN75_19395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY8976390.1"
                     /translation="MTVQIRHLATAAADFEAEFAKLRHWSEEADHAIEERVAAILADV
                     RARGDAAVLEYTNRFDGTDATSVAALELSRGELKAAFDAITPAQRAALESAARRVRSY
                     HERQLDACGRSWQYRDEDGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHVAG
                     VGEIVMVVPTPRGERNPLVLAAAFVAGVHRVFTVGGAQAVGALAYGTATIPAVDKITG
                     PGNAYVASAKRRVFGQVGIDMIAGPSEILVLADGTTPADWVAMDLFSQAEHDELAQSI
                     LLCPDAGYLAQVQAAIDRLIGEMPREAVIRNSLKDRGALIHTRSMDEACEISNRIAPE
                     HLEVSSAEPAKWEPLLRHAGAIFLGAYTSESLGDYCAGPNHVLPTSGTARFSSPLGVY
                     DFQKRSSLIQVSEAGAHILGPIAAELAYGEGLQGHARAAEMRLRKKP"
     gene            complement(2564..>3110)
                     /gene="hisG"
                     /locus_tag="VIN75_19400"
     CDS             complement(2564..>3110)
                     /gene="hisG"
                     /locus_tag="VIN75_19400"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY8976391.1"
                     /translation="ILATNQPDVRVVLVRATDVPTYVQHGGADLGVAGKDVLIEHAAS
                     VDGTSGLYQPLDLQIARCRMSVATRADFDYEAAVKQGSRIRVATKYTAVARQHFADKG
                     VHVDLIKLYGSMELAPLTGLADAIVDLVSTGSTLKANKLVEVERIMDISSRLVVNQAA
                     LKLKRERIRKLIDAFAGAIKA"
BASE COUNT          436 a         1053 c         1117 g          504 t
ORIGIN      
        1 gcgtcagcag gctgatcaac tcgtccgagc cgttgcccag catgatgtcg cagtcggcgg
       61 gcatgttcgc gtgcttcgcg agctcggcgc gcaggacgtc gccgcgctcg gccgggtagc
      121 ggttcagcgc cacctcggcc aggcgttcgc cgagttcctt gcgcagcgcg tcgggcaggc
      181 ggaacgggtt ttccatcgtg tcgaccttga cgaagccggc actgggctgg acggcgtagc
      241 cgtgcatgcc ctggacgtcg gcgcgcagga agcgggacat gcggtcggac aaggtgctca
      301 tgcagcggct ccaagggtgt cgaacgacga ggcgaacgga tcgaccacgg tcagggactc
      361 gatctgccgg ccgtgtgtcc agcggtcgga ggcgacgaag cggcactcct gctgctgcgc
      421 cgcggccagg atcagcgcat cggcgtacgg cagctcatgc cggctctcga tggcccaggc
      481 cgtctcgatg gtggcgtggt cgaccaccca cggcttccag tgctggtagc ggcgcacgag
      541 ggcgcgggcg tcgccggcgg agatcgcggt ggggaagtgg cggcgggcgt gggcgtagaa
      601 ctcggacagc acctgcacgc tggtacggcc gcagcgggcc gtccagcagc cggccagcca
      661 cgcgcgggca cggtcccgcc gctcggggtc gtggtcgtcc acggcgtgca gcagcacgcc
      721 ggtatcgacg aagatgcgcg gcgacgcgac gggcggcacg gcgctcatcg gctcatcccg
      781 cgttcgaacg gacggccgtc ggagcggccg aagctcttga attccagcgc ctcgcgcatg
      841 gcgcgctcgt aggagtcgtc atggcgcatc ttctcggcca gcagttcgcc gatcagccgt
      901 gacacgctcg tgttgcgacg cgccgcctcc atgcgtgccc agtcggcgac ctggtcttcc
      961 atggtgacgg tgacgttctt cattgcagga attccggctt gaacacgaac ttcgtgccac
     1021 actagtttcg tgcgacacga acttcgtgtc aagcgtcgcc acttcggtca cgtggccgtc
     1081 cggccgaggt ggatcctgcg actgcgcgca ggatgacgcc gcctcttcgg tcacatcctc
     1141 cggcgctgac gagccaacta cttcgtcatc ctgtgcgcag tcgcaggatc caccccggcc
     1201 aaccaggtcg tcgccctcag ggcttcttcc gcaaacgcat ctccgccgct cgcgcatggc
     1261 cctgcagccc ttcgccataa gccagctccg ccgcgatcgg ccccaggatg tgggcgcccg
     1321 cttcgctgac ctggatcagg ctgctgcgct tctggaagtc gtagacgccc agcggcgacg
     1381 agaagcgcgc ggtgcccgac gtcggcagca cgtggttcgg gccggcgcaa tagtcgccca
     1441 ggctctcgct ggtgtaggcg cccaggaaga tcgcgcccgc atggcgcagc agcggctccc
     1501 acttcgcggg ctcggcgctg ctgacttcca ggtgctcggg tgcgatgcga ttgctgatct
     1561 cgcaggcctc gtccatcgag cgcgtgtgga tcagcgcccc gcgatccttc agcgagttgc
     1621 ggatcaccgc ctcgcgcggc atctcgccga tcaggcgatc gatggccgcc tgcacctgcg
     1681 cgaggtagcc cgcatcgggg cacagcagga tgctctgagc gagctcgtcg tgctcggcct
     1741 ggctgaacag gtccatcgcg acccagtcgg ccggcgtcgt gccgtcggcc agcacgagga
     1801 tctcgctggg gccggcgatc atgtcgatgc cgacctggcc gaacacgcgg cgcttggcgc
     1861 tggcgacata cgcgttgccc gggcccgtga tcttgtcgac cgcgggaatc gtggcggtgc
     1921 cgtacgccag cgcgcccacg gcctgcgcgc cgccgacggt gaacacgcgg tgcacgccgg
     1981 cgacgaaggc cgcggccagc accagcgggt tgcgctcgcc gcgcggcgtg ggcacgacca
     2041 tgacgatctc gccgacgccc gccacgtgcg cggggatcgc gttcatcagc acgctggacg
     2101 gataggccgc cttgccgccg ggcacgtaga tgccgacgcg atcgagcggc gtgaccttct
     2161 ggcccagcag cgtgccgtcc tcgtcgcggt actgccagct gcgaccgcag gcgtcgagct
     2221 ggcgctcgtg gtagctgcgc acgcggcgcg cggccgactc cagcgcggcc cgctgcgctg
     2281 gcgtgatggc gtcgaatgcc gccttgagtt cgccgcgcga cagctccagc gcggccacgc
     2341 tcgtcgcgtc cgtgccgtcg aagcggttcg tgtactccag caccgcggcg tcgccgcggg
     2401 cgcgcacgtc ggccaggatc gcggcgacgc gctcctcgat cgcatgatcg gcttcctcgg
     2461 accaatggcg cagcttggcg aactcggctt cgaagtcggc ggcggcggtg gcgaggtggc
     2521 ggatctggac cgtcatggcg atgctgcgtg aaagggttga ggatcaggcc ttgatcgcgc
     2581 ccgcgaaggc gtcgatcagc ttgcggatgc gttcgcgctt caacttgagc gcggcctggt
     2641 tgacgaccag gcgcgaggag atgtccatga tgcgctcgac ctcgaccagc ttgttggcct
     2701 tgagcgtcga gccggtggag acgaggtcga cgatcgcgtc ggccaggccg gtcagcgggg
     2761 ccagctccat cgagccgtac agcttgatga ggtcgacgtg cacgcccttg tcggcaaagt
     2821 gctggcgcgc gacggcggtg tacttggtgg cgacgcggat gcgcgagccc tgcttgaccg
     2881 cggcctcgta gtcgaagtcc gcgcgcgtgg ccacgctcat gcggcagcgg gcgatctgca
     2941 ggtccagggg ctggtacagg ccgctggtgc cgtcgacgga tgcggcgtgc tcgatcagga
     3001 cgtccttgcc ggccacgccg aggtcggcgc cgccgtgctg cacgtaggtc ggcacgtcgg
     3061 tggcgcgcac caggacgacg cgcacgtcgg gctggttcgt cgccaggatc
//
LOCUS       DATCRO010000719         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_660795, whole genome shotgun sequence.
ACCESSION   DATCRO010000719 DATCRO010000000
VERSION     DATCRO010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660795"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VIN75_19405"
     CDS             complement(<1..711)
                     /locus_tag="VIN75_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976392.1"
                     /translation="MGDMPDFASTSLISTLPEPAPARRADGVWRAAWRRFRRDGVGMV
                     SLAVVLAYVVLIACTAGGLVARGWQDEVGQPDAPPSFAGAAAREATVAASAAAAAASA
                     RANVDISDVDPLASRYKEWDERAAQYHAQARPVAATLTFGADRLGRDVLAKALKGTQI
                     SVSVGVLAAIVATLIGAALGAVAGFFGGRVGDVVEWVYNVLTAVPEILLFFAFVAVFG
                     RGISTLVLIMGLCGWTGQY"
     gene            complement(722..1660)
                     /locus_tag="VIN75_19410"
     CDS             complement(722..1660)
                     /locus_tag="VIN75_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976393.1"
                     /translation="MLAYLLRRLWQMIPTLLGVVLLVFFLFRKFGVDPAQTLASLNAT
                     PREIAEIRQQLGLNEPWWVQLGLFIKGIVTFDWGRSWATNETIAHLMATRLPATLTVT
                     IPILILDTLVAIPIALGVAYVRGSLTDRAVMVGTTVALSVSFLVYVIAGQYVFGFQLG
                     WFPVQGWSDSVWTNLLVYAPLPVLLAVLVGLSPQTRLYRSFFLDELGHDYVRTARAKG
                     LTEPAVLYRHVLRNALIPILTNVGVALPGVLLGSFLIEVYFSIPGIGREVLLAVNRSD
                     YPVIQAVTIYVAMLTMLTNLAVDLSYRAVDPRVVLR"
     gene            1785..3596
                     /locus_tag="VIN75_19415"
     CDS             1785..3596
                     /locus_tag="VIN75_19415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976394.1"
                     /translation="MRPSIRRCWPFALLAAWAVAGGQALPAPAPKVLRYAMEVAETGF
                     DPAQVPDAYSREIIDNIIEPPLRYDLLARPLKLRVATAAALPEMSPDFRELTLRIRPG
                     IFFADDPAFHGRARELTADDYAYTLKRHFDPALHSVAYADLLGDDILGLQALRERAQN
                     RGQPFDYSAPVEGLRVLDRYTLRIRFGHPNPRFPLKLAETVYAGAIAREVAETYGDAI
                     MEHPVGTGPYRLVQWRRSSFIALERNPRFRDEFYDEQPAAGDADGQAIAARLHGRREP
                     MIDRVEVSIIEEPQPSWLAYVGGAFDFTRVPYEFAATAAPDGRLAPNLERRGMHLHQV
                     AQPDVLYTYFNMDDPVVGGYTPERVALRRAISLGYDNAEEIRLLRKPLAIPAQGLVAP
                     LVAGYDPGYRSGMNDYDPVRARALLDLYGYVDANGDGWRERPGGGALVLHMATQPDSN
                     ARQLNELWQKKMEAIGIRIVFDRRPWPEQMKQARAGKLQMWTLGASSDEPDPEDLLML
                     GYGPDKGEGNWSRFDLPEYNAAWRRIKDLPDGPERSAAIVQAQKLLLAWMPLKTHLHR
                     VRLVLAQPWLEGWPANPFVNGFWRFMDIDTSRRPVAH"
     gene            3658..>4704
                     /locus_tag="VIN75_19420"
     CDS             3658..>4704
                     /locus_tag="VIN75_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976395.1"
                     /translation="MTDIEPRASAAAVAHRWLAVLVLGFASGLPLALTGLAMQAWLTM
                     DGLDLAAIGFLTLIGIPYTFKFLWAPLLDRVDLPLLGRRRGWIVLTQALLAFALFALA
                     DVDPKLSLPLFSALGVLTAFLSASQDIVVDAWRVDVLPPRERGFGSSLFVMGYRIAMI
                     LSGGVALMWTDTVDGIGMTWPAVYRLMGVIMAVLAVATALALPRVAQPPARLRDASRG
                     PIRQDLAGFLAVVAAAAAGVWLTQRFGGPIAGALLSPWFGHDVARGSLQAKWIDLLAL
                     VLGLAVTLPLAGLAARRANFQTLLAGLDSYFSQPRAWIFLAFIVFYKLTDAFQLSLLT
                     PFLLKGMAFGPAEVG"
BASE COUNT          675 a         1731 c         1634 g          664 t
ORIGIN      
        1 gtactggccg gtccagccgc acaggcccat gatcagcacc agcgtggaga tgccgcgccc
       61 gaacaccgcg acgaacgcga agaacagcag gatctcgggc accgcggtca gcacgttgta
      121 gacccactcg acgacgtcgc ccacccggcc gccgaagaag ccggcgacgg cgccgagcgc
      181 cgcgccgatc agcgtggcca cgatcgccgc cagcacgccc accgacaccg agatctgcgt
      241 gcccttcaac gccttggcga gcacgtcgcg ccccagccgg tcggcgccga aggtcagcgt
      301 ggcggccacg gggcgggcct gcgcgtggta ctgcgcggcg cgctcgtccc actccttgta
      361 gcgcgacgcg agcgggtcga cgtccgagat gtcgacgttg gcccgcgccg aggccgccgc
      421 ggcggcggcc gacgcggcga ccgtcgcctc gcgcgcggcc gcacccgcga acgacggcgg
      481 cgcgtcgggc tggcccacct cgtcctgcca gccgcgcgcc accagcccgc cggccgtgca
      541 cgcgatcagg acgacgtacg ccagcacgac agccagcgac accatgccca cgccgtcgcg
      601 ccggaagcgc cgccacgcgg cgcgccacac gccgtccgcg cgccgcgccg gcgcgggctc
      661 gggcagcgtc gagatgagcg acgtcgaggc gaagtcgggc atgtccccca tcgcggcgtc
      721 ctcagcgcag caccacgcgc gggtcgaccg cgcggtacga caggtcgacg gcgagattgg
      781 tcagcatcgt cagcatcgcc acgtagatcg tgaccgcctg gatgacgggg tagtcgctgc
      841 ggttgacggc cagcagcacc tcgcggccga tgcccgggat ggagaagtac acctcgatca
      901 ggaacgagcc gagcaggacg cccggcagcg cgacgccgac gttggtcagg atggggatca
      961 gcgcgttgcg cagcacgtgg cgatagagca cggccggttc ggtcaggccc ttggcgcgcg
     1021 cggtgcgcac gtagtcgtgg cccagctcgt cgaggaagaa gctgcggtac aggcgcgtct
     1081 gcggcgacag ccccaccagc accgccagca gcaccggcag cggcgcgtag accagcaggt
     1141 tcgtccatac gctgtcgctc cagccctgaa ccgggaacca ccccagctgg aagccgaaca
     1201 cgtactggcc ggcgatcacg tacacgagga acgagaccga cagcgccacc gtcgtgccca
     1261 ccatgacggc gcggtcggtg agcgaacccc gcacatacgc cacgcccagc gcgatgggga
     1321 tggcgaccag cgtgtccagg atcaggatcg ggatcgtgac cgtcagcgtg gccggcaggc
     1381 gcgtggccat caggtgggcg atggtctcgt tggtggccca gctgcggccc cagtcgaacg
     1441 tgacgatgcc cttgatgaac aggcccagct gcacccacca cggttcgttc aggccgagct
     1501 gctggcggat ctccgcgatc tcgcgcggcg tcgcattcag gctcgccaga gtctgcgccg
     1561 ggtcgacgcc gaacttgcgg aacaggaaga acacgagcag caccacgccc agcagcgtgg
     1621 gaatcatctg ccagagacga cgaagcaggt aggcgagcat cgagccgggc gcgggtgagg
     1681 cggcgaggaa acgcgagttt aggggttgta gactccacgt cgctcgggaa tcgccctgcg
     1741 gcgcccgctg tcgtcaactt cccacacagg ctccggagtc gctcgtgcgt ccgtcgatcc
     1801 gtcgttgctg gccgttcgcg ctgctcgccg cctgggccgt cgcgggcggc caggcgttgc
     1861 cggcgccggc cccgaaggtg ctgcgctacg cgatggaggt ggccgagacc ggcttcgacc
     1921 ccgcgcaggt gcccgacgcc tactcgcgcg agatcatcga caacatcatc gagccgccgc
     1981 tgcgctacga cctgctcgcg cgtccgctca agctgcgcgt ggccaccgcc gcggcgctgc
     2041 cggagatgtc gcccgacttc cgcgagctca cgctcaggat ccgccccggc atctttttcg
     2101 ccgacgaccc ggccttccac ggccgcgccc gcgagctgac ggccgacgac tacgcctaca
     2161 cgctcaagcg ccatttcgac ccggcgctgc acagcgtggc ctacgccgac ctgctcggcg
     2221 acgacatcct gggcctgcag gcgctgcgcg agcgcgcgca gaaccgcggc cagccgttcg
     2281 actacagcgc gccggtcgag ggcctgcgcg tgctcgatcg ctacacgctg cgcatccgct
     2341 tcggccaccc gaacccgcgc ttcccgctca agctcgccga gacggtctac gccggcgcga
     2401 tcgcccgcga ggtggccgag acctacggcg acgcgatcat ggagcacccc gtgggcaccg
     2461 gcccgtaccg gctggtgcag tggcggcgct cgtcgttcat cgcgctggag cgcaacccgc
     2521 gcttccgcga cgagttctac gacgagcagc ccgccgccgg cgacgccgac ggccaggcga
     2581 tcgccgccag gctgcacggc cggcgcgagc cgatgatcga ccgcgtcgag gtgtccatca
     2641 tcgaggagcc ccagccgtcg tggctcgcct acgtcggcgg cgcgttcgac ttcacgcgcg
     2701 tgccctacga gttcgccgcc accgccgcgc ccgacggccg cctcgcgccc aacctcgagc
     2761 gccgcggcat gcacctgcac caggtggcgc agcccgacgt gctctacacg tacttcaaca
     2821 tggacgaccc ggtcgtcggc ggctacacgc ccgagcgcgt ggcgctgcgg cgcgccatct
     2881 ccctgggcta cgacaacgcc gaggagatcc gcctgctgcg caagccgctc gcgatccccg
     2941 cgcaggggct ggtcgcgccg ctggtggccg gatacgaccc ggggtaccgc agcgggatga
     3001 acgactacga tccggtgcgg gcccgcgcgc tgctcgacct ctatggctac gtcgacgcca
     3061 acggcgacgg ctggcgcgag cgtcccggcg gcggcgcgct ggtgctgcac atggccacgc
     3121 aacccgattc caacgcacgc caactcaacg agctgtggca gaagaagatg gaggcgatcg
     3181 gcatccgcat cgtgttcgac cgccgcccgt ggcccgagca gatgaagcag gcgcgcgccg
     3241 gcaagctgca gatgtggacg ctgggcgcct cgtccgacga gccggatccc gaggacctgc
     3301 tgatgctggg gtacggcccc gacaagggcg agggcaactg gtcgcgcttc gacctgcccg
     3361 agtacaacgc cgcctggcgc cgcatcaagg acctgcccga cggccccgag cgcagcgccg
     3421 ccatcgtcca ggcgcagaag ctgctgctcg cctggatgcc gctcaagacg cacctgcatc
     3481 gcgtgcgcct cgtgctggcg cagccatggc tcgagggctg gccggccaac ccgttcgtca
     3541 atggcttctg gcggttcatg gacatcgaca cgtcgcgccg gccggtggcc cattgacgcg
     3601 ctctcgccag acccctgcgg gtgcgacccg ttccgccgtg ccggcgacaa tgccccgatg
     3661 accgacatcg aaccccgcgc gtccgccgcg gccgtcgccc accgctggct cgccgtgctc
     3721 gtgctcggct tcgcctcggg gctgccgctg gcgctgaccg gcctggccat gcaggcctgg
     3781 ctgacgatgg acggcctgga cctggcggcg atcggcttcc tgacgctgat cggcattccc
     3841 tacacgttca agttcctgtg ggcgccgctg ctcgatcggg tcgatttgcc gctgctggga
     3901 cgccgccgcg gctggatcgt gctcacgcag gcgttgctgg cgttcgcgct gttcgcgctg
     3961 gccgacgtcg acccgaagct gtcgctgccg ctgttctccg ccctgggggt gctcaccgcc
     4021 ttcctcagcg cgtcgcagga catcgtggtc gacgcctggc gcgtggacgt gctgccgccg
     4081 cgcgagcgtg gcttcggctc gtccctgttc gtgatgggct accgcatcgc gatgatcctg
     4141 tcgggcggcg tcgcgctgat gtggaccgat acggtggacg gtatcggcat gacctggccg
     4201 gcggtctacc ggctgatggg ggtgatcatg gcggtgctgg cggtggcgac cgcgctggcg
     4261 ttgccgcgcg tcgcgcagcc gcccgcgcgc ttgcgtgacg cgtcgcgcgg gccgatccgc
     4321 caggatctcg ccggcttcct cgcggtggtc gctgcggcgg ccgcgggcgt ctggctgacc
     4381 cagcgcttcg gcggccccat cgccggcgcg ctgctgtcgc cctggttcgg ccacgacgtg
     4441 gcgcgaggct cgctgcaggc caagtggatc gacctgctcg cactggtgct gggcctggcg
     4501 gtgacgctgc cgctggccgg cctggcggcg cggcgcgcga atttccagac gctgctggcg
     4561 ggcctggact cgtacttcag ccagccgcgc gcctggatct tcctggcgtt catcgtgttc
     4621 tacaagctca ccgacgcgtt ccagctgtcg ctgctgacgc ccttcctgct caagggcatg
     4681 gcgttcggcc cggcggaggt gggc
//
LOCUS       DATCRO010000720         7290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_41948, whole genome shotgun sequence.
ACCESSION   DATCRO010000720 DATCRO010000000
VERSION     DATCRO010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41948"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VIN75_19425"
     CDS             complement(<1..727)
                     /locus_tag="VIN75_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976396.1"
                     /translation="MLLLPALGLRPLRLFLLGALLLGVLGLLLLRALLLGPLRLLLLR
                     ALLRLLLRALLGLLLGTLLLGALRLLPLRALLLGPLRLLLLRALLRLLLRALLGLLLL
                     RLLLLLLLRPHLIGALGLLLRGLLLRLFLLGLLLGLRLLRAHLVRTLGLLLRGLLLRL
                     LLLGPLLLRLRLLRPHLVGALGLFLHRALLRLILRRARVGLRRRTGALRAGGGGRQVL
                     QVDVLAAGTRLAGLGGRHGGMGHL"
     gene            complement(853..1092)
                     /locus_tag="VIN75_19430"
     CDS             complement(853..1092)
                     /locus_tag="VIN75_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY8976397.1"
                     /translation="MLLLKPGCECCDVDLPPDSMDARICSFECTFCAPCAAKLGRCPN
                     CGGELLRRPIRPAGKLAANPASTTRVHKPAGCASA"
     gene            complement(1224..2864)
                     /locus_tag="VIN75_19435"
     CDS             complement(1224..2864)
                     /locus_tag="VIN75_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8976398.1"
                     /translation="MKTVLLGAALLSGATVALADDHRHAIEHVLIISVDGMHAQDLAR
                     CLADRTCPHIASLARHGVTYANAFTPGLSDSVPGLAALVTGGGPRSTGLFYDDVYDRT
                     LYAGTDTQCAGKPGVEVLLQEQVGIDAMNGGALSHLDGGGAFNPQQIPRRKVGSQCVP
                     VYPHDFIQANTIFEVVKQHLPRAHTAWSDKHAWGTDWVNGPSGRGVDDLARTEINSID
                     GPAGNDFTGSYDGVAPAYLHTEAFDGIHVDNILHQIDGKDSTGAVAMPVPTIFGTNFQ
                     TLSVAQKAPHAAGGGYADAAFTPNPFVRDAMGWVDGAIGRIEGELAARRLADSTLVVL
                     TAKHGQSPADHARLMKIGHAVGATLGSYAGGGSDPVTGNNAGNGQVTDDDVAFVWLND
                     QAQRETVKALLQASTACPVVSPVNHTVAAVAPKICADNGGAVLDLAAMPWKFGDPAGG
                     RTPDFMIQPNPGVIYTTSQAKDQEHGGFAPDDGHVALLVSHPSFEARTVERRVQTTQV
                     APTVIRALGLDPRLLLAVRKEGTRVLPHALQDERDDDN"
     gene            3147..3410
                     /locus_tag="VIN75_19440"
     CDS             3147..3410
                     /locus_tag="VIN75_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976399.1"
                     /translation="MAHANPHDTQIKALEGRLNMLGDKLASLVGHSQQVGELIPIIHR
                     PGWTTVAELALVAGLVESMIKHTELIGDLHATLMRGAQAVGKH"
     gene            complement(3426..4229)
                     /locus_tag="VIN75_19445"
     CDS             complement(3426..4229)
                     /locus_tag="VIN75_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY8976400.1"
                     /translation="MTIGPQRIVCLTEETTEWLYLLGEERRIVGISGYTVRPRRARDE
                     KPRVSAFLDGKIDKIVALEPDLVIGFSDMQAALADKLIRAGLNVFITNQRSVAEIFAT
                     LRLVAGLVQAGDRAEAWIAACQARHAAIAAASASWPRRPRVYFEEWDDPMISAIRWVS
                     ELVAVAGGIDVFEELGRHAGGKDRIVHDPAEPPRRAPDLIVGSWCGKKFRGANVAARP
                     GWQDVPAVANGELHEIKSCDILQPGPAALTDGVEQLHRLCAAWAAKQGG"
     gene            complement(4270..5550)
                     /locus_tag="VIN75_19450"
     CDS             complement(4270..5550)
                     /locus_tag="VIN75_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976401.1"
                     /translation="MKTTRAHRHAAAACAFLSLACAALPALAANNNPVILVHGFAGFG
                     RTELLGYKYWGGLNDLQEQLKARYPDQLVATAVVGPFSSNWDRAVELFYQIKGGCVDY
                     GALHASANGHYQRPESYYHNGRTCYPGLYPAWDASHPVHLISHSMGGQTSRMLVQMLA
                     ANGAPSNPSLFPYAVNANWIRSVTTIATPNDGTTLAYRITDWVPFVQQLVTGIANFTG
                     ATGGNLVYDFKLDQWGVGAQAPGESFSAYVNRVTSSPLWNSGVKDLSPYDLSPAGAMA
                     ENDWVKDQPNVTYFSWVTNSSYSGLLTGWSYPDLDTNALIAAFAGPGFMGNYYRDSRP
                     MPPVDDSTWFPSDAVVNTVAQKAPTWAMNSSGVVYKRSVVVRDISGGGTPQAGAWNYK
                     GTLGGFDHLDAVGWTLFWDSLSWYRARVDELRAL"
     gene            5879..7153
                     /locus_tag="VIN75_19455"
     CDS             5879..7153
                     /locus_tag="VIN75_19455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976402.1"
                     /translation="MAQAHEASTPRHAAHPFLFLVLFIPFGAVPGFLTVALAYELKKK
                     GIDTSDIAIVVGLSYICLLLKWLWAPLVDTLLTRKTWYVGSTLVSSLGFWLAGKAAMG
                     EHPSLVAVSAWLTISNFMSTLLAMAVESLMAATVPDDQRGRAGGWSMAGNLGGQGLGG
                     GLSLWLIESAGFSSAASGALLGLLCALCCVALAWVDDARPLLKVEGHVEAAGHKGVAA
                     NLRAIGTDLWELLRSRVGILALMICLLPIGSGAAQNLWSPIADEWHASAGVVAFVTGA
                     MSGLISAAGCLAGGWLCDRMDRKTAYCLFGFLLCVVAAGMALCPRTSGQYIFWTSAYA
                     FVVGLCYAAYCAVVLEAIGKTAAATKFSFLSAVSNSPIMLMTFVDGWAQGRFGTNGML
                     WTEAATGVVGIVVFAAVAGATKRRQAGAALAT"
BASE COUNT         1067 a         2544 c         2645 g         1034 t
ORIGIN      
        1 ccagatggcc catgcccccg tgacgcccac cgaggcccgc caggcgcgtg ccggcggcaa
       61 ggacgtcgac ctgcagcacc tgccgcccac cgccgccggc ccgcagggcg cccgtccggc
      121 gccgcagtcc aacccgcgcg cggcgcagga tcaggcgcag cagcgcgcgg tgcaggaaca
      181 ggcccagcgc gccgaccaga tgcgggcgca gcaggcgcag tcgcagcagc agagggccca
      241 gcaggagcag gcgcagcaga aggccgcgca ggagcaggcc cagcgtgcgg accagatgcg
      301 cgcgcagcag tcgcaggccc agcagaaggc ccagcaggaa caggcgcaac agaaggccgc
      361 gcaggagcag gcccagcgcg ccgatcagat gcgggcgcag caggagcagc agcagaaggc
      421 gcagcaggag caggcccagc agcgctcgca gcagcaggcg cagcaacgcg cgcagcagga
      481 gcaggcgcaa cgggccgagc aacagcgcgc gcagcgggag caggcgcagc gcgccgagca
      541 gcagcgtgcc cagcaggagg cccagcagcg ctcgcagcaa caggcgcagc aacgctcgca
      601 gcaggagcag gcgcaacggg ccgagcaaca gcgcgcgcaa caggagcagg cccagcacgc
      661 cgagcagcaa cgcacccagc aggaacaggc gcagcgggcg caggcccagc gcggggagca
      721 gcagcacccg ccgcaggaac gcggcgagcg cgcccgcccg aacgaggaag gcgcgagccg
      781 gccgcagcgc tgacctggtc cggcagacgg gcggcgtccg gcggtgtcag gccgcccggc
      841 gccgcccttt tctcaggcgg acgcgcagcc cgcgggcttg tgcacgcgcg tcgtcgatgc
      901 cggattcgcg gccagcttgc cggccggccg gatcggtcgc cgcagcagtt ctccgccgca
      961 gttggggcag cgtcccagct tggccgcgca cggcgcgcag aacgtgcatt cgaacgagca
     1021 gatgcgcgca tccatcgagt cgggcggcag gtcgacgtcg cagcattcgc agccgggctt
     1081 gagcagcagc atggcgtttc cttggtcggg aaccgcagtc tcgccccggc gccgctcgaa
     1141 caccaggccg cacagggacg atccgcggac gcttgcggac atcgcgcgcg tcacgcggcc
     1201 ccgcgcgcgg ggccgcggcg ggctcagttg tcgtcgtcgc gctcgtcctg cagcgcatgc
     1261 ggcagcacgc gcgtgccttc cttgcgcacg gccagcagca ggcgcgggtc gaggccgagg
     1321 gcacggatca cggtcggcgc gacctgggtc gtctgcacgc ggcgctccac cgtccgcgcc
     1381 tcgaacgacg gatgcgagac cagcagcgcg acatgcccgt cgtccggcgc gaagccgccg
     1441 tgctcctggt ccttggcctg gctcgtggtg tagatcacgc ccgggttcgg ctggatcatg
     1501 aagtcgggcg tgcggccgcc ggcggggtcg ccgaacttcc agggcatggc ggccaggtcg
     1561 aggacggcgc cgccgttgtc ggcgcagatc ttcggcgcca ccgcggccac cgtgtggttg
     1621 acggggctca cgaccgggca ggccgtcgac gcctgcagca gcgccttgac cgtctcgcgc
     1681 tgcgcctggt cgttcagcca gacgaacgcg acgtcgtcgt cggtgacctg gccgttgccc
     1741 gcgttgttgc cggtgaccgg atcgctgccg ccgccggcgt agctccccag cgtcgcgccg
     1801 accgcgtggc cgatcttcat caggcgcgca tggtcggcgg gcgactggcc atgcttggcg
     1861 gtcagcacca ccagcgtcga gtcggcgaga cggcgcgcgg cgagctcgcc ctcgatcctt
     1921 ccgatcgcgc cgtccaccca gcccatcgcg tccctgacga acgggttcgg cgtgaaggcc
     1981 gcgtcggcgt agccgccgcc ggcggcatgg ggcgccttct gcgcgacgct gagcgtctgg
     2041 aagttggtgc cgaagatcgt cgggacgggc atcgcgacgg cgccggtcga atccttgccg
     2101 tcgatctggt ggaggatgtt gtcgacatgg atcccgtcga aggcctccgt gtgcaggtag
     2161 gccggcgcga cgccgtcata cgagccggtg aagtcgttgc cggcggggcc gtcgatcgag
     2221 ttgatctcgg tgcgcgccag gtcgtccacg ccccggcccg acggaccgtt gacccagtcg
     2281 gtgccccagg cgtgcttgtc cgaccacgcg gtgtgcgcgc gcggcaggtg ctgcttgacg
     2341 acctcgaaga tcgtgttcgc ctggatgaag tcgtgcgggt acaccggcac gcactggctg
     2401 ccgaccttgc gccgcgggat ctgctgcggg ttgaacgcac caccgccatc gaggtgcgac
     2461 agcgcgccgc cgttcatcgc gtcgatgccc acctgctcct gcagcagcac ttccacgccc
     2521 ggcttgcccg cgcactgcgt gtcggtgccg gcatacagcg tgcggtcgta gacgtcgtcg
     2581 tagaacaggc ccgtggagcg cggaccgccg ccggtcacca gggcggccag gcccggcacc
     2641 gagtccgaca ggccgggcgt gaacgcgttg gcgtaggtca cgccgtgacg cgcgagcgag
     2701 gcgatgtgcg ggcaggtcct gtccgcgagg cagcgcgcca ggtcctgcgc gtgcatgccg
     2761 tcgacgctga tgatgagcac atgctcgatg gcgtgacgat ggtcgtcggc cagggcgacg
     2821 gtggcgccgg acagcagcgc cgcgccgaga aggactgtct tcatggtgtc tcgatgggtt
     2881 ggttcggggg acgcggcgat gcgagtcgcc gccccggcaa tctcgcgacg cgagatgacc
     2941 ggggcgtgac gacgctgtcg gaaaggcatc gccgaacttg cgcggatctg aagcgatgag
     3001 cacgcgaccg cgccgccggg ccgcccctag ttccgtcgcg ttccctagcc cgggggagtg
     3061 cggagcgaca gcgcctgcct agactggttc gagcgatgcc cggggcgtcg cacatcccac
     3121 cagcacctat caccgcagga gacgccatgg cccacgccaa cccgcacgac acgcagatca
     3181 aggccctcga aggccgcctc aacatgctgg gcgacaagct cgccagcctc gtcggccact
     3241 cgcagcaggt cggcgagctg atccccatca tccatcgtcc cggctggacg acggtggccg
     3301 aactggcgct cgtcgccggc ctcgtggaat cgatgatcaa gcacaccgag ctgatcggcg
     3361 acctgcacgc gacgctgatg cgcggcgcgc aggcggtggg caagcactga acggctcgcg
     3421 ggcgctcagc cgccctgctt cgccgcccag gccgcgcaca ggcgatgcaa ctgctcgacg
     3481 ccgtcggtca gcgcggccgg gccgggttgc aggatgtcgc aggacttgat ctcgtgcagc
     3541 tcgccgttgg ccacggccgg cacgtcctgc cagcccgggc gcgccgccac gttggcgccg
     3601 cgaaacttct tgccgcacca gctgccgacg atgaggtcgg gcgcgcggcg cggcggctcg
     3661 gcaggatcgt ggacgatgcg gtccttgccg ccggcgtgcc ggcccagctc ctcgaacacg
     3721 tcgatgccac cggctacggc gacgagctcc gacacccagc ggatcgcgct gatcatcgga
     3781 tcgtcccatt cctcgaagta gacgcgcggg cgccgcggcc acgacgccga cgccgccgcg
     3841 atcgccgcat ggcgggcctg gcaggcggcg atccacgcct cggcgcggtc gccggcctgc
     3901 acgagccccg cgaccagccg cagcgtcgcg aagatctccg ccacgctgcg ctggttcgtg
     3961 atgaacacgt tcaggccggc gcggatcagc ttgtcggcga gcgcggcctg catgtcggaa
     4021 aagccgatga cgaggtcggg ctcgagcgcg acgatcttgt cgatcttgcc gtcgaggaag
     4081 gccgacacgc gcggcttctc gtcgcgcgcc cgccgcggcc gcaccgtgta gccggagatg
     4141 ccgacgatgc ggcgctcctc gccgagcagg tagagccact cggtggtctc ttcggtgagg
     4201 cagacgatgc gttgcgggcc gatggtcatg cgggcattgt cgcccgtcga aacgtgcggc
     4261 ccgcccggct caaagcgccc gcaactcgtc cacgcgcgcc ctgtaccagg acaggctgtc
     4321 ccagaacagc gtccagccca cggcgtcgag gtggtcgaag ccgcccagcg tgcccttgta
     4381 gttccacgcg ccggcctgcg gcgtgccgcc gccgctgatg tcgcgcacca ccaccgaacg
     4441 cttgtagacg acgcccgacg agttcatcgc ccaggtcggt gccttctggg cgaccgtgtt
     4501 gacgaccgcg tcgctcggga accacgtgct gtcgtcgacc ggcggcatcg ggcgcgagtc
     4561 gcggtagtag ttgcccatga aaccggggcc ggcgaatgca gcgatcagcg cgttggtgtc
     4621 gaggtccgga taggaccagc cggtgagcag gccgctgtag ctcgagttgg tcacccacga
     4681 gaagtaggtg acgttgggct ggtccttgac ccagtcgttc tcggccatcg cccccgcggg
     4741 cgagaggtcg tacggcgaca ggtccttgac gcccgagttc cacagcgggc tgctcgtcac
     4801 gcggttgacg taggcgctga agctctcgcc cggggcctgc gcgcccacgc cccattgatc
     4861 gagcttgaag tcgtagacga ggttgccgcc ggtcgcgccc gtgaagttgg cgatgccggt
     4921 gaccagctgc tgcacgaacg gcacccagtc cgtgatgcgg taggccagcg tggtgccgtc
     4981 gttgggggtg gcgatcgtcg tgacgctcct gatccagttc gcgttgaccg cgtacgggaa
     5041 cagcgacggg ttgctcggcg cgccgttggc cgcgagcatc tgcaccagca tgcgcgaggt
     5101 ctgcccgccc atgctgtgcg agatcaggtg caccgggtgg ctggcgtccc acgcggggta
     5161 caggccggga tagcaggtgc ggccgttgtg gtagtagctc tcggggcgct ggtagtggcc
     5221 gttggccgac gcatgcagcg cgccgtagtc cacgcagccg cccttgatct ggtagaacag
     5281 ttcgacggcg cgatcccagt tcgagctgaa cgggccgacc accgcggtgg ccacgagctg
     5341 gtccgggtac ctcgccttca attgctcctg caggtcgttc aggccacccc agtacttgta
     5401 gcccagcagc tcggtgcggc cgaagccggc aaagccgtgc acgaggatca cggggttgtt
     5461 gttggcggcc agcgcgggca gcgccgcgca agcgagcgag aggaacgcgc atgcggcagc
     5521 cgcatgcctg tgggcacgag tcgtcttcat gatgtctccg ggtttgtttt gatggcgaga
     5581 gacaccgggc aggacccgcc ggcgctccca tgggccgtcg acatcggggc tgctttgcac
     5641 atccgcggac gtcggcgcat cggctggcgg ccctcgtgag gccacggcgt gatgctgtcc
     5701 ctgtcgccgc ccgcgcacac tggccgcgcg ggccaagctg ttgtcccgcg catcgtcgtg
     5761 ttgcaccggc atcgcccgaa cgcggtatgc gtcggcggcc gcgggcccgc tcgctatccc
     5821 gggggctcgc gcggcagtgg cgcggggaca atcgcgcgtc gaccgctcga aagcccccat
     5881 ggcccaggcc catgaagcca gcacgccccg ccacgcggcg cacccgttcc tgttcctcgt
     5941 gctgttcatc ccgttcggcg cggtgccggg cttcctcacc gtggcgctcg cctacgagct
     6001 caagaagaag ggcatcgaca cctccgacat cgccatcgtc gtcggcctga gctacatctg
     6061 cctgctgctc aagtggctgt gggccccgct cgtcgacacc ctgctcacgc gcaagacctg
     6121 gtacgtcggc tcgacgctcg tcagcagcct gggcttctgg ctggcgggca aggcggcgat
     6181 gggcgagcac ccgtcgctgg tggcggtcag cgcgtggctg acgatcagca acttcatgtc
     6241 cacgctgctg gcgatggccg tcgagagcct gatggcggcc accgtgcccg acgaccagcg
     6301 cggccgcgcc gggggctggt cgatggcggg caacctgggc ggccaggggc tgggcggcgg
     6361 cctgagcctg tggctgatcg agagcgccgg cttcagcagc gcggcctcgg gagccctgct
     6421 gggcctgctg tgcgcgctgt gctgcgtcgc gctggcgtgg gtcgacgacg ccaggcccct
     6481 gctgaaggtc gaaggccacg tcgaggccgc cggccacaag ggcgtcgccg ccaacctgcg
     6541 cgcgatcggc accgacctgt gggagctgct gcgctcgcgc gtgggcatcc tggcgctgat
     6601 gatctgcctg ctacccatcg gcagcggagc ggcgcagaac ctgtggtcgc cgatcgccga
     6661 cgagtggcac gcgtcggccg gcgtcgtcgc cttcgtcacc ggagcgatga gcggcctgat
     6721 ctccgcggcg ggctgcctcg cgggcggctg gctgtgcgac cgcatggacc gcaagaccgc
     6781 ctactgcctg ttcggcttcc tgctgtgcgt cgtcgcggcg ggcatggcgc tgtgcccgcg
     6841 cacgtccggc cagtacatct tctggacctc ggcctacgcg ttcgtcgtgg gcctgtgcta
     6901 cgccgcgtac tgcgcggtgg tgctggaggc catcggcaag acggccgccg ccaccaagtt
     6961 cagcttcctg tcggccgtgt cgaactcgcc catcatgctg atgaccttcg tcgacggctg
     7021 ggcgcagggc cggttcggga cgaacggcat gctgtggacg gaagcggcga ccggggtcgt
     7081 gggcatcgtc gtgttcgcgg cggtggcggg ggcgacgaaa cgccgccagg ccggcgcggc
     7141 gctcgccacc tgacgcgaac caggggcatg cgacaggccc tgcgcacggg cgctgccgag
     7201 gtgcctggcc ttgcatgttg cacgctcgga caacggcgat tgccgatacg gcagccgtgc
     7261 gacatgcgag acctggcacc gaacgtgcac
//
LOCUS       DATCRO010000721         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_413144, whole genome shotgun sequence.
ACCESSION   DATCRO010000721 DATCRO010000000
VERSION     DATCRO010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413144"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VIN75_19460"
     CDS             complement(<1..1692)
                     /locus_tag="VIN75_19460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8976403.1"
                     /translation="MSSDPAATVSALEAQLLVDTFRGIDLGTVWLDEDARIVLANDHF
                     AGWAGIDAATLAGRAMADLFADFNGTRWAEIAHAADGDGAPPRLTLTAPGAAPRVLEV
                     RAQRVAHDGRALVALLLVPIPDRVEAEAIDGLQREVLESIALGRPLRAVLDLLCRRVE
                     TLAPDVACSVLLIEKNGTVTPIAGPSLAPAYVDALHGAPIGPRAGSCGTAAWRREAVE
                     VTDIATDPLWADYRALLPLLDKAACWSTPIIAGNGDVVATFALYYRRPGSAPPFHRRM
                     VEACVPLCRVALQHEANRSEIERLAYFDPLTGLPNRRLFSDRARQTLQMAVRIQAPGA
                     LLLFDIDRFKTTNDSLGHGAGDEVLRETAKRLQSEIGDSATVARLGGDEFAAMLPRCS
                     PVEAMHAAERLREALAAPLRISGMQIEVSTSIGIALFPQDGSELEGLVKNAEMAMYEV
                     KRGGRGASRFFTSMMNDVVAQRMQMESALRQALAARGLALHYQPKMHLDGSGCAGVEA
                     LARWRDPVLGDVPPDRFIALAEECGLIGAIDAWVLETACAQLAAWQRDDVPVPSIS"
     gene            1916..2176
                     /locus_tag="VIN75_19465"
     CDS             1916..2176
                     /locus_tag="VIN75_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY8976404.1"
                     /translation="MNKRLIATSTLASALALAAGHAQADDKAAAKEKCYGNVKAGQND
                     CATGTHSCAGQAKADNDASDWKFVAKGTCEKLGGSTKAPAKK"
     gene            complement(2213..2992)
                     /locus_tag="VIN75_19470"
     CDS             complement(2213..2992)
                     /locus_tag="VIN75_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8976405.1"
                     /translation="MTASVFGRRPDDRPPREEDWSTGVTISIVAHATLIGALVWGLHW
                     RSSTQPTEASAELWAAIPETAAPPPVETPPPPPAPAPVPAPAPVEPPKPPDIVVEQIK
                     KPPPKPTPAPPPPKPAPPPPAPKPAPAPPPEKLDAKTLQKMREENLKRMMGQMNAPVD
                     ATGTGQRNAGPSASYLGRIVARLKPNAVVTDPIPGNPIVEVEIRCAPDGTILSRRITK
                     SSGNKAWDDTVVRAIDKTGKLPVDTDGKALDRIPIAWGPQD"
     gene            complement(3003..>3409)
                     /locus_tag="VIN75_19475"
     CDS             complement(3003..>3409)
                     /locus_tag="VIN75_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEY8976406.1"
                     /translation="RGRGRRSISEINMVPFIDVMLVLLIIFMVSAPLITTGVVDLPSM
                     GRAKQRPPSVIEVIVGTDEQIRIRVDKTDPQPTTLKALAARVGELQNGRADVPVIIAG
                     DKAVRYESIVKVMDTLQKAGVQRVGLAVKQLG"
BASE COUNT          456 a         1224 c         1259 g          470 t
ORIGIN      
        1 cgagatgctg ggcaccggca cgtcgtcgcg ctgccaggcg gccagctgcg cgcaggccgt
       61 ctccagcacc cacgcgtcga tcgcgccgat caggccgcat tcctcggcca gcgcgatgaa
      121 gcggtcgggc gggacgtcgc cgagcaccgg atcgcgccaa cgcgccagcg cctcgacgcc
      181 ggcgcagccg ctgccgtcca ggtgcatctt gggctggtag tgcagcgcca gcccgcgcgc
      241 ggccagcgcc tgccgcagcg cgctctccat ctgcatgcgc tgcgcgacca cgtcgttcat
      301 catgctcgtg aagaagcgcg aggcgccgcg cccgccgcgc ttgacctcgt acatggccat
      361 ctcggcgttc ttgaccagcc cctccagctc gctgccgtcc tgcgggaaca gcgcgatgcc
      421 gatgctggtg gacacctcga tctgcatgcc cgagatgcgc agcggcgcgg ccagcgcctc
      481 gcgcaaccgc tcggcggcgt gcatcgcctc gaccgggctg cagcgcggca gcatcgccgc
      541 gaactcgtcg cctccgaggc gcgcgacggt ggcgctgtcg ccgatctcgg actgcagccg
      601 cttggcggtc tcgcgcagca cctcgtcgcc ggcgccgtgg cccagcgagt cgttggtggt
      661 cttgaagcgg tcgatgtcga acagcagcag cgcgcccggt gcctggatgc gaaccgccat
      721 ctgcagcgtc tggcgcgcgc ggtcgctgaa caggcgccgg ttgggcaggc ccgtgagcgg
      781 atcgaagtag gccaggcgct cgatctcgga acgattggcc tcgtgctgca gcgcgacgcg
      841 gcacaggggc acgcaggcct cgaccatgcg gcgatggaac ggcggcgccg agccgggacg
      901 gcggtagtac agcgcgaagg tggcgacgac gtcgccgttg ccggcgatga tgggcgtcga
      961 ccagcacgcc gccttgtcca gcagcggcag cagcgcgcgg tagtcggccc acagcgggtc
     1021 ggtggcgatg tcggtgacct ccaccgcctc gcgccgccag gccgccgtgc cgcacgagcc
     1081 cgcgcgcggc ccgatcggcg cgccgtgcag cgcatcgacg taggccggcg ccaggctggg
     1141 cccggcgatc ggcgtgacgg tgccgttctt ctcgatcagc agcaccgagc aggcgacgtc
     1201 cggcgccagc gtctcgacgc gccggcagag caggtcgagc accgcgcgca gcgggcggcc
     1261 cagcgcgatc gattccagca cctcgcgctg cagcccgtcg atggcctcgg cctcgacgcg
     1321 gtcggggatg ggcaccagca gcagcgcgac cagcgcgcgg ccgtcgtgcg cgacgcgctg
     1381 cgcgcgcacc tcgagcacgc gcggggccgc gccgggggcg gtgagcgtca ggcgcggcgg
     1441 cgcgccgtcg ccgtcggcgg cgtgggcgat ctcggcccag cgcgtgccgt tgaagtcggc
     1501 gaacaggtcg gccatcgcgc ggccggcgag cgtcgccgca tcgatgccgg cccagccggc
     1561 gaagtggtcg ttggccagca cgatccgcgc atcctcgtcc agccagacgg tccccaggtc
     1621 gataccccga aacgtatcga ccagcagctg ggcctcgagg gcggagacgg tggcggccgg
     1681 atcgctggac aagcacggac tccctggttg gatcacgagc ggtggcgcgt ggctcggatg
     1741 attgtgcgag ccggggcagg gcgccgcaac gccccgcctg tggggacttc tggacgagac
     1801 gcccacgcgg cgcgggcgtc cgccgctcga cagcttgaac ctccgccccg ccgcgaggta
     1861 caaacggagc atcccgcggg cccgcgcccg ccccgccgac acaaggatcg tctccatgaa
     1921 caagcgcctc atcgccacct cgacgctcgc ctccgcgctc gccctcgccg ccggccacgc
     1981 ccaggccgac gacaaggccg cggccaagga gaagtgctac ggcaacgtca aggcgggcca
     2041 gaacgactgc gcgaccggca cgcactcgtg cgcgggccag gccaaggccg acaacgacgc
     2101 cagcgactgg aagttcgtcg ccaagggcac gtgcgagaag ctcggcggct cgaccaaggc
     2161 gcccgcgaag aagtgatcgg ccgatccgcg ggcgagggcc gctcgccccg gctcagtcct
     2221 gaggccccca ggcgatgggg atccggtcga gcgccttgcc gtcggtgtcg accggcagct
     2281 tgccggtctt gtcgatggcc cgcaccacgg tgtcgtccca cgccttgttg ccgctggact
     2341 tggtgatgcg ccggctcagg atcgtgccgt ccggcgcgca gcggatctcg acctcgacga
     2401 tcgggttgcc cgggatcgga tcggtcacca ccgcgttcgg cttgagccgc gccacgatgc
     2461 ggcccagata gctcgccgac ggcccggcgt tgcgctggcc cgtgccggtc gcgtcgaccg
     2521 gggcgttcat ctggcccatc atgcgcttga ggttctcctc gcgcatcttc tgcagcgtct
     2581 tggcgtcgag cttttccggc ggcggcgccg gtgccggctt gggtgcgggc ggcggcggcg
     2641 cgggcttggg cggcggcgga gccggggtcg gcttgggcgg cggtttcttg atctgctcga
     2701 cgacgatatc cggcggcttc ggcggttcca ccggcgcggg tgccggaacg ggagcgggcg
     2761 ccggcggggg cggcggcgtc tcgaccggcg gcggcgcggc cgtctcggga atggcggccc
     2821 acagctcggc cgaggcctcg gtcggctggg tcgagctgcg ccagtgcagg ccccacacca
     2881 gcgcgccgat cagcgtcgca tgggcgacga tcgagatggt gacgccggtg ctccagtcct
     2941 cctcgcgcgg aggacggtcg tcggggcggc ggccgaagac gctggcggtc atgtcgctcc
     3001 ggtcagccca gttgcttgac ggcgaggccg acgcgctgga cgcccgcctt ctgcaaggtg
     3061 tccatcacct tgacgatgct ctcgtagcgc accgccttgt cgcccgcgat gatcacgggc
     3121 acgtcggcgc gcccgttctg cagctcgccc acgcgcgcgg cgagcgcctt gagcgtcgtc
     3181 ggctgcgggt cggtcttgtc gacgcggatc ctgatctgct cgtcggtgcc cacgatcacc
     3241 tcgatgaccg acggcgggcg ctgcttggcg cggcccatcg aaggcaggtc gacgacgccg
     3301 gtggtgatca gcggcgcgct gaccatgaag atgatcagca gcaccagcat gacgtcgatg
     3361 aacggcacca tgttgatctc gctgatcgag cggcggccgc ggccgcggg
//
LOCUS       DATCRO010000722         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_345833, whole genome shotgun sequence.
ACCESSION   DATCRO010000722 DATCRO010000000
VERSION     DATCRO010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345833"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VIN75_19480"
     CDS             <1..828
                     /locus_tag="VIN75_19480"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8976407.1"
                     /translation="FEPHPRDFFARRAGKPELAPARISTLRDQVVELERCGIDHVILL
                     RFDEHLAGLPPDDFIANVLIKGLGVRYVLVGDDFRFGARRAGDYAMLDAAGSRLGFDV
                     ARMLSYEVHGLRVSSSAVREALAAGDMAKAASLLGRPYSVSGRVAHGSKLGRELGATK
                     RGAGDGFRTLNLRMPEARPAASGIFVSRVHGLTPEPLPAVSSLGVRPTIEKAGRVLLE
                     THCLAWPDPLGREAAYGRLINVELVKWLHEERKYDSLDALQAGIAADVIAARDFFGL"
     gene            complement(1104..2327)
                     /locus_tag="VIN75_19485"
     CDS             complement(1104..2327)
                     /locus_tag="VIN75_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HEY8976408.1"
                     /translation="MPLSTPLQLPNGAVIPNRVAKASMEENMADAGQVPGPAIRALYA
                     AWAAGGAGLILTGNVMIDGRAMTGPGGIVLERGTPREPFAAWARDARAHGAQVWMQIN
                     HPGRQVFAAMKGEAWAPSAVALDLGKHSGLFAAPREMTEADIAEVIGRFADTARAAEE
                     SGFTGVQIHAAHGYLISQFLSPLANRRADRWGGSLANRARLLLEVVKAVRAKVSPGFC
                     VSVKLNSADFQRGGFSENDARQVLLWLNDLSVDLVELSGGSYEAPAMQGRAADGRTLA
                     REAYFLEFAKDLAAAATMPVMTTGGIRRRAVAEQVLASGVAMVGVATALALRPDMPKA
                     WFAGKDLDATPVAVTWKDKGMAGLANMALVKRRLRAVAAGQGAPRAYSPLFTLIADQL
                     RTKGLVRRYRAWAGR"
     gene            2432..2932
                     /locus_tag="VIN75_19490"
     CDS             2432..2932
                     /locus_tag="VIN75_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8976409.1"
                     /translation="MKIGELAAATGLAPSAIRFYEQSGLLPAAERSANGYRSYSPEAV
                     ERLRYIQLAQALGFTLDALRANIVTRAGQTRDEIHEDLLQRLDTRLVEIDRMLATLRA
                     QRKDLLAVRAQMEASWAEGECLDAASVTLTPVEHPAPVPTLPRARRVTREGAPAPAPA
                     RRVQRR"
     gene            3173..>5598
                     /gene="ileS"
                     /locus_tag="VIN75_19495"
     CDS             3173..>5598
                     /gene="ileS"
                     /locus_tag="VIN75_19495"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY8976410.1"
                     /translation="MTADTDKTTPDYRATLNLPDTPFPMRGDLPKREPGWVAQWEKDG
                     LYKRLRDARVGKPLFVLHDGPPYANGQIHMGHAVNKVLKDMIVKTRQLMGFDAQYAPG
                     WDCHGLPIENAIEKEFGRKLSRDDMQARSRAYATAQIGQQMADFRRLGVLGDWEHPYR
                     TMDFANEAGELRAFKRVIERGFVYRGLKPVYWCFDCGSSLAEFEIEYADKKSFTIDVG
                     FKCAEPEKLAAAFKLPRLDKDAFVVIWTTTPWTIPANQALNLNPQLEYSLVDTERGLL
                     LLASALVDKCMERYGLTGQVVATTIGQNLDEIKFRHPFAEVDKGYDRLSPIYLADYAT
                     ADDGTGIVHSSPAYGVDDFNSCVSHGMKIDEILNPVQGSGVYAPELPLFGGQFIWKAQ
                     QAIVDALRDAGRLLATSQITHSYPHCWRHKTPVIYRAAAQWFVRMDEGQGVFTKDKAP
                     ATLRQLALEAIDQTSFYPENGKARLRDMISGRPDWCISRQRSWGVPIPFFLHKDTGEL
                     HPKTMEILDQAADIVERGGIEAWSRVTVEEMVGAADAPNYTKSTDILEVWFDSGSTFF
                     HVLRGTHAGTPEHPGFHSQGPEADLYLEGHDQHRGWFHSSLLIACALYGRAPYKGLLT
                     HGFTVDSQGRKMSKSLKNGVDPQETCSKLGAEIIRLWVAASDYSGDIAGDDKILARVV
                     DSYRRIRNTLRFLLANTSDFDPQSDAVPLGDLLEIDRYALARAAQLQGEILEHFQVYE
                     FHPVVSKLQIYCSEDLGAFYLDVLKDRLYTTAPKSFARRSAQTALWHITHAMLRWMAP
                     MLSFTAEEAFP"
BASE COUNT          867 a         2047 c         1887 g          797 t
ORIGIN      
        1 ttcgagccgc atccgcgcga cttcttcgcg cgccgcgccg gcaagccgga gctcgcgccg
       61 gcgcgcatct ccacgctgcg cgaccaggtg gtggagctcg agcgctgcgg catcgatcac
      121 gtcatcctgc tgcgtttcga cgagcacctg gccggcctgc cgcccgacga cttcatcgcc
      181 aacgtgctga tcaagggcct gggggtgcgc tacgtgctgg tgggcgacga cttccgcttc
      241 ggcgccaggc gcgcgggcga ctacgcgatg ctcgacgccg ccggcagccg cctcggcttc
      301 gacgtcgcgc gcatgctgtc gtacgaggtg cacgggctgc gcgtgtccag ctccgcggtg
      361 cgcgaggcgc tggccgccgg cgacatggcc aaggccgcgt cgctgctggg ccggccgtac
      421 tcggtcagcg gacgcgtcgc ccatggcagc aagctcggac gcgaactcgg cgccacgaag
      481 cgcggcgccg gcgacggctt tcgcacgctc aacctgcgca tgcccgaagc caggcccgcg
      541 gcatcgggca tcttcgtctc gcgcgtgcac ggcctgacgc ccgagccgct gccggccgtc
      601 tccagcctgg gcgtgcggcc caccatcgag aaggcgggcc gcgtgctgct ggagacgcac
      661 tgcctggcct ggcccgaccc gctgggccgg gaggcggcct acggcaggct gatcaacgtc
      721 gagctggtca agtggctgca cgaggagcgc aagtacgact cgctcgacgc gctgcaggcg
      781 ggcatcgcgg cggatgtcat cgcggcgcgg gacttcttcg gtctctgatc gagggcctgg
      841 ttcgcacaag tggatcctgc gactgcgcgc cggatgacgg cttggccttg cgtggccttg
      901 gggtctggcc ttggggtcag gtcttgaatg ttgcgcggca atgcatcggc atcggggtca
      961 ggtcttgaat gttgcacgag gagccgtcgt tccatggcga ggcgtcctct gtgcaacatt
     1021 caagacctga cccccagcgc ggtgctcaac gcgcaacatt caagacctga ccccgaggag
     1081 ttcgatagcg cgccggacgg ctctcagcgg ccagcccagg cccggtagcg cctgaccagc
     1141 cccttcgtgc gcagctggtc ggcgatcagc gtgaacagcg gcgaataggc gcgcggggcg
     1201 ccctgccccg ccgcgacggc gcgcaggcgg cgcttgacca gcgccatgtt cgccagcccc
     1261 gccatgccct tgtccttcca ggtgacggcc accggcgtcg catcgagatc cttgcccgcg
     1321 aaccaggcct tgggcatgtc cggacgcagc gccagcgcgg tcgccacgcc caccatcgcc
     1381 acgccgctgg ccagcacctg ctcggcgacc gcgcggcggc ggatgccgcc cgtggtcatg
     1441 acgggcatcg tggcggcggc agcgagatcc ttggcgaact ccaggaagta ggcctcgcgc
     1501 gccagcgtgc ggccgtcggc ggcgcggccc tgcatggcgg gcgcctcgta gctgccgccg
     1561 gacagctcca ccaggtcgac ggacaggtcg ttcagccaca gcagcacctg acgcgcgtcg
     1621 ttctccgaga atccgccgcg ctggaagtcg gccgagttca gcttgaccga cacgcagaag
     1681 cccggcgaca ccttggcccg caccgccttg accacctcca gcagcaggcg cgcgcgattg
     1741 gccagcgagc cgccccagcg gtcggcgcgg cggttggcca gcggcgacag gaactgcgag
     1801 atcaggtagc cgtgcgcggc gtggatctgc acgccggtga acccggactc ctcggcggcg
     1861 cgggcggtgt cggcgaagcg gccgatgacc tcggcgatgt cggcctcggt catctcgcgc
     1921 ggcgcggcga acaggccgct gtgcttgccc aggtccagcg ccaccgccga cggcgcccag
     1981 gcttcgccct tcatggcggc gaacacctgg cggcccggat ggttgatctg catccacacc
     2041 tgcgcgccgt gcgcacgcgc gtcacgcgcc caggcggcga agggctcgcg cggcgtgccg
     2101 cgctccagca cgatgccgcc ggggccggtc atggcgcggc cgtcgatcat cacgttgccg
     2161 gtcaggatca ggcccgcgcc gccggccgcc cacgcggcgt acagcgcgcg gatcgccggc
     2221 ccgggcacct gtccggcgtc ggccatgttc tcctccatcg aggccttggc cacgcggttg
     2281 gggatcaccg cgccgttggg cagctgcagc ggcgtcgaca ggggcaggga agcgggcgat
     2341 ttcatgggtt tcgaggcctt ggaaatttgc gaagtgccgc catgctagga ttcaagtcaa
     2401 cttgaaggtc aagcactttc tggaacccgc catgaagatc ggcgaactcg ccgccgccac
     2461 cggcctcgca ccgtccgcga tccgcttcta cgagcagagc ggcctgctgc ccgccgccga
     2521 acgctcggcc aacggctacc gcagctattc gcccgaggcg gtggagcggc tgcgctacat
     2581 ccagctcgcc caggcgctcg gcttcaccct ggacgcgctg cgcgccaaca tcgtcacccg
     2641 cgccgggcag acccgggacg agatccacga ggacctgctg cagcgcctgg acacccgcct
     2701 cgtggagatc gaccggatgc tcgcgacgct gcgtgcccaa cgcaaggacc tgctggccgt
     2761 gcgcgcccag atggaggcga gctgggccga gggcgagtgc ctggacgcgg ccagcgtgac
     2821 gctgacgccg gtcgagcacc cggcgccggt tcccacgctc ccacgcgcgc gccgcgtcac
     2881 gcgcgagggc gccccggcgc ccgcgcccgc gcgccgggtc cagcgccggt agaatggacg
     2941 ccatgtcgct gccttccagc cttcgtctcg ccacctgccg ctggcacgcc gccacgcggc
     3001 gccaggccac gcgcgaccga attattggcg ggctcgcctc ggcctgagcc caccaatgac
     3061 cgcacgcgtt ccgagcgacg cggtcatccg gcacccttcg ccgtcccgct gacggccccg
     3121 gcctccaacg ccacagcctc ttcgcgcgtc gccgcctgac gcgcctcccg ccatgaccgc
     3181 cgacaccgac aagaccaccc ccgactaccg cgcgacgctc aacctgcccg acacgccgtt
     3241 cccgatgcgc ggcgacctgc ccaagcgcga gccgggctgg gtcgcgcagt gggagaagga
     3301 cggcctgtac aagcgcctgc gcgacgcccg cgtcggcaag ccgctgttcg tgctgcacga
     3361 cggcccgccg tacgccaacg gccagatcca catggggcac gccgtcaaca aggtgctcaa
     3421 ggacatgatc gtcaagaccc gtcagctgat gggcttcgac gcgcagtacg cgccgggctg
     3481 ggactgccac ggcctgccga tcgagaacgc gatcgagaag gaattcggcc gcaagctcag
     3541 ccgcgacgac atgcaggcca ggagccgcgc ctacgccacc gcgcagatcg gccagcagat
     3601 ggccgacttc aggcgcctgg gcgtgctcgg cgactgggag catccgtacc gcacgatgga
     3661 cttcgccaac gaggccggcg agctgcgcgc gttcaagcgc gtgatcgaac gcggcttcgt
     3721 ctatcgcggc ctcaagcccg tgtactggtg cttcgactgc ggctcgtcgc tggccgagtt
     3781 cgagatcgag tacgccgaca agaagagctt cacgatcgac gtcggcttca agtgcgccga
     3841 gcccgagaaa ctggccgccg cgttcaagct gccgaggctg gacaaggacg ccttcgtcgt
     3901 gatctggacg accacgccgt ggacgatccc cgccaaccag gcgctgaacc tgaacccgca
     3961 gctggaatac agcctggtcg acaccgagcg cggcctgctg ctgctcgcgt ccgcgctggt
     4021 cgacaagtgc atggagcgct acgggctcac gggccaggtc gtcgccacca cgatcggcca
     4081 gaacctggac gagatcaagt tccgccatcc gttcgcggag gtcgacaagg gctacgaccg
     4141 cctgtcgccg atctacctgg ccgactacgc cacggccgac gacggcaccg gcatcgtcca
     4201 ctcgtcgccc gcctacggcg tcgacgactt caactcgtgc gtcagccacg gcatgaagat
     4261 cgacgagatc ctgaacccgg tgcagggctc gggcgtgtac gcgccggagc tgcccctgtt
     4321 cggcggccag ttcatctgga aggcgcagca ggccatcgtc gacgcgctgc gcgacgccgg
     4381 ccgcctgctt gcgacctcgc agatcaccca cagctacccg cactgctggc gccacaagac
     4441 gccggtgatc taccgcgccg cggcgcagtg gttcgtgcgc atggacgagg gccagggcgt
     4501 cttcaccaag gacaaggcgc cggccacgct gcgccagctc gcactcgagg cgatcgacca
     4561 gaccagcttc tatcccgaga acggcaaggc gcggctgcgc gacatgatct cgggccgccc
     4621 cgactggtgc atctcgcgcc agcgcagctg gggcgtgccg atcccgttct tcctgcacaa
     4681 ggacacgggc gagctgcatc cgaagacgat ggagatcctc gaccaggccg ccgacatcgt
     4741 cgagcgcggc ggcatcgagg cctggagccg cgtcaccgtc gaggagatgg tgggcgccgc
     4801 cgacgcgccg aactacacca agagcaccga catcctcgag gtctggttcg actccggctc
     4861 cacgttcttc cacgtgctgc gcggcacgca cgccggcacg ccggagcatc cgggcttcca
     4921 ctcgcagggc cccgaggccg acctgtacct ggaaggccac gaccagcacc gcggctggtt
     4981 ccactcgtcg ctgctgatcg cctgcgcgct gtacggccgc gcgccctaca agggcctgct
     5041 gacgcacggc ttcacggtgg acagccaggg ccgcaagatg agcaagtcgc tcaagaacgg
     5101 cgtcgatccg caggagacct gcagcaagct gggcgccgag atcatccggc tgtgggtggc
     5161 cgcgtcggac tattcgggcg acatcgccgg cgacgacaag atcctcgccc gcgtggtcga
     5221 ctcgtaccgc cgcatccgca acacgctgcg cttcctgctc gccaacacga gcgacttcga
     5281 tccgcagtcg gacgcggtgc cgctgggcga cctgctcgag atcgaccgct atgcgctggc
     5341 gcgggccgcg cagctgcagg gcgagatcct ggagcacttc caggtctacg agttccaccc
     5401 cgtcgtctcc aagctgcaga tctactgctc ggaggacctg ggcgcgttct acctggacgt
     5461 gctcaaggac cgcctgtaca cgacggcgcc caagtcgttc gcgcgccggt cggcgcagac
     5521 cgcgctgtgg cacatcacgc acgcgatgct gcgctggatg gcgccgatgc tcagcttcac
     5581 ggccgaggag gcgttccc
//
LOCUS       DATCRO010000723         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_278535, whole genome shotgun sequence.
ACCESSION   DATCRO010000723 DATCRO010000000
VERSION     DATCRO010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278535"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..915)
                     /locus_tag="VIN75_19500"
     CDS             complement(250..915)
                     /locus_tag="VIN75_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8976411.1"
                     /translation="MSFEPIRLHVPPPFELWLVSLASPAGDADFDLLDDAERARSARF
                     HFERDRGRYAAAHAGLRRLLAQRTGRDATSLAIAAGPYGKPYLADAPRCAFSLSHCED
                     LALVALADDGEIGVDLEHVRPLSDLDGLARQCLTLHERHVLDALAPDDRHLAFLRRWT
                     RKEACLKALGTGLHIEPSTFCVDGGGTDEQVCVQTISGPRDVHIQDVDVRPGWVGALA
                     RVV"
     gene            1063..2928
                     /locus_tag="VIN75_19505"
     CDS             1063..2928
                     /locus_tag="VIN75_19505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY8976412.1"
                     /translation="MSSSQRRRAAIVAAAIALACVVALALPPARHALVAAIPPDRLVQ
                     LNGLRFGYGVERDLRLPMPDGVHLGASLYLPRRHDEKLATILVRLPYGRLEYGEALNS
                     AEYWARRGYAVLVEDLRGTHGSEGELVPYRSGMTDGAATLDWIVAQPWSNGRVGTVGC
                     SALGETQYVLARTHHPALRAMIPQGAGGAIGSAAGRYAYFGLWEGGVFQLASGFGWFV
                     DDGAKDPHAPPAAPFDVASAVRGLPVADLVRRQRPAPNGYDDFMRMPPTDPAWHDLGY
                     LADGDRVAAPTFEVTTWGDQTVGDTLAFDAQQRLPIDGKPAPERHLIVAAGTHCHEQE
                     TGESDRFGEIAVPHADAPLREWYERWFDHWLRDRGDGLASLPAIRWYQIGEGRWLDTS
                     SWPPPQARMQRWYLDSGGHANGAKGDGVLAPAAPLQSAHDDYVHDPDTPVPSRGGPMC
                     CTGNPADHPGPADQKDVERRDDVLVYTSPALTTPLRIAGPLKATLRFSSSAVDTDVVA
                     RLVDVWPDGRATSIQEGALRARYRTGVPTPTLLVPGEPVTLTVDMRSIGYTLPAGHRL
                     RLDIASSSFPRLERNLGTGRDNLHETQVVVARNRVLHGADALSYVELPLLPAATD"
     gene            complement(2889..3749)
                     /locus_tag="VIN75_19510"
     CDS             complement(2889..3749)
                     /locus_tag="VIN75_19510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8976413.1"
                     /translation="MTEPTETIARFGADERLLGIVTLPAQRRADAPACLLMNAGVVHR
                     IGPHRINVKIARALAALGMASIRIDLSGLGDSPPASGGMHSSTQTVMDLQAAMDHLES
                     TLGVHRFIVFGLCSGSVHAYWLAQKDPRIAGVVMFDSYVFPTFKTHVLRRWRRLRTQS
                     WGSLARKPLEWLRRGPRVKPRAEGEESTDPHMKYPTRADFARVMNDLTGRGMSIYLYY
                     SASFLEKFNYHGQMHDLFGREPFMQRVRYDYETDIDHTVTSLHAQRKVLASVVDWAQS
                     VAAGSSGSST"
BASE COUNT          553 a         1440 c         1376 g          523 t
ORIGIN      
        1 ccaccggcgt ggtggaagca gaacagccgg cgcgcggcgt cgggccgagg tgcaaggcgt
       61 tggagccaat tgctcacgat gggcttccgg aggcggcgca tgaaaccgtg tcaccgaggc
      121 ctcgcgttcc gctcaacgca ctccccccgg cggcgggcgt cgcagcatcc gcgcgcctgc
      181 cttgtccttt tttctcatca taaaccttag ctcaacttga acgcggtggc attccccata
      241 agtggctagt cacacgacgc gcgcgagcgc gcccacccag ccgggacgca cgtcgacgtc
      301 ctggatgtgc acgtcgcgcg gaccggaaat ggtctggacg cacacctgct cgtccgtgcc
      361 gccgccgtcg acgcagaagg tcgacggctc gatgtgcagg cccgtgccca gcgccttcag
      421 gcaggcttcc ttgcgcgtcc agcggcgcag gaacgcgagg tggcgatcgt cgggcgcgag
      481 ggcatcgagg acgtggcgct cgtgcagcgt caggcactgc cgggcgaggc cgtccaggtc
      541 ggacagcgga cgcacgtgct cgaggtcgac gccgatctcg ccgtcgtcgg ccagcgccac
      601 cagcgccagg tcctcgcaat ggctcaggct gaaggcgcag cgcggtgcgt cggccagata
      661 cggcttgccg tacgggccgg cggcgatcgc cagcgacgtc gcgtcgcgcc ccgtgcgctg
      721 cgcgagcagg cgccgcagcc ccgcgtgcgc ggcggcgtag cggccgcgat cgcgctcgaa
      781 atggaagcgc gccgagcgcg cgcgctcggc gtcgtccagg aggtcgaagt cggcgtcgcc
      841 ggcgggcgac gcgaggctca ccagccacag ctcgaacggc ggcgggacat gcaggcggat
      901 cggttcgaac gacatggcgg ggacgcgaag tcagccacga cattgtcgac ccggcgcccc
      961 gcccggcgcc atgcaatctc gacgcgggcg cgcagccttt cacgccaccc ctgcggcgat
     1021 actgcgcgct gggccgcgca gggtcgcgga aggaggagtc ccgtgtcgtc atcccagcgc
     1081 cgaagagccg ccatcgtcgc ggccgccatc gcgctcgcgt gcgtcgtcgc cctggcgctg
     1141 ccgcccgcgc ggcacgcgct cgtggcggcc atcccgcctg accgcctcgt gcagctgaac
     1201 ggcctgcgct tcggctacgg cgtcgagcgc gacttgcggc tgcccatgcc cgatggagta
     1261 cacctgggcg ccagcctcta cctgccgcgc cgtcatgacg agaagctcgc caccatcctc
     1321 gtgcgcctgc cctatggccg tctcgagtac ggcgaggcgc tcaattcggc cgaatactgg
     1381 gcgcgccgcg gctacgccgt gctcgtggag gacctgcgcg gcacccacgg ctccgagggc
     1441 gagctggtgc cgtatcgctc cggcatgacc gacggcgcgg ccacgctcga ctggatcgtc
     1501 gcccagccgt ggtcgaacgg acgcgtcggc accgtcggct gcagcgcgct gggcgagacc
     1561 cagtacgtcc tcgcgcgcac ccaccacccg gcgctgcgcg cgatgatccc gcagggcgcg
     1621 ggcggcgcga tcggcagcgc ggcggggcgc tacgcctact tcggcctgtg ggaaggcggc
     1681 gtgttccagc tcgcgagcgg cttcggctgg ttcgtcgacg acggcgccaa ggatccgcac
     1741 gcgccgccgg ccgcgccgtt cgacgtggcg tccgccgtgc gcggcctgcc ggtggccgac
     1801 ctcgtccggc gccagcgccc ggcgcccaac ggctacgacg acttcatgcg catgccgccg
     1861 acggacccgg cctggcacga cctcggctac ctggccgacg gcgaccgcgt cgccgcgccc
     1921 accttcgagg tcacgacctg gggcgaccag accgtgggcg acacgctggc cttcgacgcg
     1981 cagcagcgcc tgccgatcga cggcaagccc gcgcccgagc gccacctgat cgtcgcggcc
     2041 ggcacgcact gccacgagca ggagaccggc gaaagcgacc gcttcggcga gatcgccgtc
     2101 ccgcacgccg atgcgccgct gcgcgagtgg tacgagcgct ggttcgacca ctggctgcgc
     2161 gaccgcggcg acggcctcgc gtcgctgccc gcgatccgct ggtaccagat cggcgaaggc
     2221 cgctggctcg acacctcgag ctggccgccg ccgcaggcca ggatgcagcg ctggtacctg
     2281 gacagcggcg gccacgccaa cggcgccaag ggcgacggcg tcctcgcgcc ggcggcgccg
     2341 ctgcagtccg cgcacgacga ctacgtccac gaccccgaca cgcccgtgcc ctcgcgcggc
     2401 ggtccgatgt gctgcaccgg caacccggcc gaccacccgg gcccggccga ccagaaggac
     2461 gtcgagcggc gcgatgacgt gctggtctac acctcgccgg cgctcacgac gccgctgcgc
     2521 atcgcaggcc cgctcaaggc gacgctgcgc ttctcgtcgt cggcggtcga caccgacgtg
     2581 gtggcccggc tggtcgacgt ctggcccgac ggccgcgcga ccagcatcca ggaaggcgca
     2641 ctgcgcgccc gctaccgcac cggcgtgccc acgcccacgc tgctggtgcc cggggaaccg
     2701 gtcacgttga cggtcgacat gcgcagcatc ggctacacgc tgccggccgg ccaccgcctg
     2761 cggctggaca tcgccagcag cagcttcccg cgcctggagc gcaacctcgg cacgggccgc
     2821 gacaacctgc acgagacgca ggtcgtggtc gcccgcaacc gcgtcctgca cggcgcggac
     2881 gcgctgtcct acgtcgagct gccgctgctg ccggccgcca ccgactgagc ccagtcgacc
     2941 acgctggcca gcaccttgcg ctgcgcgtgc agcgaggtga ccgtgtggtc gatgtcggtc
     3001 tcgtagtcgt agcgcacgcg ctgcatgaac ggctcgcggc cgaacaggtc gtgcatctgg
     3061 ccgtggtagt tgaacttctc gaggaagctc gccgagtagt acaggtagat cgacatgccg
     3121 cggccggtca ggtcgttcat cacgcgcgcg aagtcggcgc gcgtcgggta cttcatgtgc
     3181 ggatcggtcg actcctcgcc ttcggcgcgc ggcttgaccc gcggtccgcg gcgcagccac
     3241 tccagcggct tgcgcgcgag gctgccccag gactgcgtgc gcaggcgcct ccagcggcgc
     3301 agcacatgcg tcttgaaggt ggggaacacg tagctgtcga acatgacgac gccggcgatg
     3361 cgcggatcct tctgcgccag ccagtaggcg tggaccgagc ccgagcacag cccgaacacg
     3421 atgaagcgat ggacgcccag cgtgctttcc aggtgatcca tcgcggcctg caggtccatc
     3481 acggtctgcg tgctggagtg catgccgccg ctggccggcg ggctgtcgcc caggcccgac
     3541 aggtcgatgc ggatgctggc catgcccagc gcggccagcg cccgggcgat cttgacgttg
     3601 atgcggtgcg gcccgatgcg gtgcaccacg ccggcgttca tcagcaggca ggcgggcgcg
     3661 tcggcgcggc gctgcgcggg cagcgtgacg atgcccagca ggcgctcgtc ggcgccgaag
     3721 cgcgcgatcg tctcggtcgg ctcagtcacg gatcgccccc atcatcgcgg ccatcgcggg
     3781 cggcggcacc agcgggctgt tgcgatcctc ttccgctgtc cagtcgaacg actcagccag
     3841 tggccaccag tgcagcgcga cgccacgggg cgagaaggtg gatgtccaag cc
//
LOCUS       DATCRO010000724         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_525678, whole genome shotgun sequence.
ACCESSION   DATCRO010000724 DATCRO010000000
VERSION     DATCRO010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525678"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIN75_19515"
     CDS             complement(<1..534)
                     /locus_tag="VIN75_19515"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY8976414.1"
                     /translation="MKAIQVGLLGIGTVGSGTYNVLTRNQEEIRRRAGRAIEITMVAD
                     LDVARAKSIVGDHAKVVADGRQIIANPDIDIVVELIGGYGIAKTLVLEAIAAGKHVVT
                     ANKALLAVHGNEIFAAAREKGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TTNFILSEMRDKGLDFDV"
     gene            complement(560..1564)
                     /locus_tag="VIN75_19520"
     CDS             complement(560..1564)
                     /locus_tag="VIN75_19520"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEY8976415.1"
                     /translation="MRRILSAIAVALGVPLAAHAQSFDSQDWTVTCDNTRTCRAAGYS
                     VEGSAMPVSLLIARAAGPNAPVYGELQLGVLAAGSVHPSSVSLAGNGKAAIAIRVDRN
                     NHAELAASALIAILKSLSAGGDVIFSSGKSTWRLSGAGAVEALQKMDDAQGRSGGGSA
                     LIRKGPAGAGDVASAMAIPRMDAARIPTAPQPDDAALAVRVLSAIQSTPDCPLLDDGA
                     SQSQGRLWHLDANRLLVTQPCKGAAGATAQGWWLANRRPPYDARPLTYAGEFDGVASI
                     TARVPAGSSGDCATAQGWTWTGYRFEQTYAATGGLCRGVKAGGAWEIPSLVADVVPAK
                     "
     gene            complement(1567..2790)
                     /locus_tag="VIN75_19525"
     CDS             complement(1567..2790)
                     /locus_tag="VIN75_19525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8976416.1"
                     /translation="MKPIAKSSKLANIGYDIRGPVLDKARQMEDEGQKIIKLNIGNVA
                     AFGLEPPDEIVQDMIRNLASAAAYTDSKGLFAPRKSVVHYTQEKGVAGVTVDDVYLGN
                     GASELISFSMNALLDSGDEVLIPSPDYPLYTAVVGLSGGTPVHYVCDEQSGWLPDLGD
                     IRRKVTPRTKAIVIINPNNPTGALYPVSVLEDIVEIARQNELIIFADEIYDKTLYDGE
                     AHTSIASLADDVLCVTFNGLSKNYRSCGYRAGWMVVSGAKKHARDYIEGLNMLASMRL
                     CANTPGQLAIQTALGGYQSIKDLVAPGGRLARQRDLAYDLLTQIPGVTVVKPKAALYM
                     FPRLDPKLYPIADDQQFAYELLAEEKVLIVQGTGFNWGQTDHFRVVFLPNADDLTEAV
                     TRIARFLDGYRKRHA"
BASE COUNT          398 a         1024 c         1063 g          494 t
ORIGIN      
        1 gacgtcgaag tcgagcccct tgtcgcgcat ctcggacagg atgaagttgg tggtgccgtt
       61 gatgatgccg gcgatccact cgatgcggtt ggcggtcagg ccctcgcgca gcgccttgat
      121 gatggggatg ccgccggcca cggcggcctc gaacgccacc atcacgcctt tctcgcgcgc
      181 agcggcgaag atctcgttgc catgcaccgc cagcagcgcc ttgttggccg tgaccacgtg
      241 cttgcccgcg gcgatggcct ccagcaccag cgtcttggcg atgccgtagc cgccgatcag
      301 ctcgacgacg atgtcgatgt cggggttggc gatgatctgg cggccgtcgg ccaccacctt
      361 cgcgtggtcg ccgacgatgg acttggcgcg cgcgacgtcg aggtcggcca ccatcgtgat
      421 ctcgatggcg cggcccgcgc ggcggcggat ctcctcctgg ttgcgcgtga ggacgttgta
      481 cgtgccggaa ccgacggtcc cgatgcccag caggccgacc tggatcgctt tcatcttcat
      541 ttcacctcgt gttgcatgct catttggcgg ggaccacgtc ggcgaccagg ctgggaatct
      601 cccaggcccc gcccgccttc acgccgcggc acaggccgcc ggtggcggca taggtctgct
      661 cgaaccggta acccgtccac gtccagccct gggccgtggc gcagtcgccg ctgctgccgg
      721 ccggcacgcg ggcggtgatg ctcgccacgc cgtcgaactc gccggcgtac gtcaggggcc
      781 gcgcgtcgta aggcgggcgg cgattcgcga gccaccaacc ctgcgccgtg gcgccggccg
      841 cgcccttgca cggctgcgtg accagcaggc ggttggcatc caggtgccac aggcgcccct
      901 gcgactgcga cgccccgtcg tccagcagcg ggcagtcggg cgtgctctgg atcgcggaca
      961 ggacgcgcac ggccagcgcg gcgtcatcgg gctgcggcgc ggtcgggatg cgggccgcgt
     1021 ccatgcgcgg gatcgccatc gccgaggcga cgtcgcccgc gcccgcgggg cccttgcgga
     1081 tcagcgccga tccgccgccg gagcggccct gcgcgtcgtc catcttctgc agcgcctcga
     1141 cggcgcccgc gccggacagg cgccaggtcg acttgcccga cgagaagatc acgtcgccgc
     1201 ccgcggacaa cgatttcagg atcgcgatca gcgcactggc cgccagctcg gcgtggttgt
     1261 tgcggtcgac gcggatcgcg atggccgcct tgccattgcc ggccaggctg acgctggacg
     1321 gatgcacgct gccggcggcg agcacgccca gctgcagctc gccgtagacc ggcgcgttgg
     1381 gtccggccgc gcgcgcgatc agcagcgaca cgggcatcgc gctgccctcg acgctgtagc
     1441 cggcggcgcg gcaggtgcgc gtgttgtcgc acgtcaccgt ccagtcctgg ctgtcgaacg
     1501 actgcgcatg cgccgcgagc ggcacgccca gcgcgacggc gatggcggaa aggatgcggc
     1561 gcatggtcag gcgtgccgct tgcggtagcc gtcgaggaag cgcgcgatgc gcgtcaccgc
     1621 ctcggtgagg tcgtccgcgt tgggcaggaa caccacgcgg aagtggtccg tctgccccca
     1681 gttgaagccg gtgccctgca cgatcagcac cttctcctcg gccagcagct cgtaggcgaa
     1741 ctgctggtcg tcggcgatcg ggtacagctt cgggtccagg cgcgggaaca tgtacagcgc
     1801 ggccttgggc ttgacgaccg tcacgcccgg aatctgcgtg agcaggtcgt aggcgaggtc
     1861 gcgctggcgc gcgaggcgcc cgcccggcgc cacgaggtcc ttgatgctct ggtagccacc
     1921 cagcgcggtc tggatcgcca gctggcccgg cgtgttggcg cacaggcgca tcgaggccag
     1981 catgttcagg ccctcgatgt agtccctggc gtgcttcttg gcgcccgaga ccaccatcca
     2041 gcccgcgcgg tagccgcacg agcggtagtt cttggacagg ccgttgaagg tgacgcacag
     2101 cacgtcgtcg gccagcgacg cgatgctggt gtgcgcctcg ccgtcgtaca gcgtcttgtc
     2161 gtagatctcg tcggcgaaga tgatcagctc gttctggcgc gcgatctcga cgatgtcctc
     2221 cagcacgctc acggggtaca gcgcgccagt cgggttgttc gggttgatga tcacgatcgc
     2281 cttggtgcgc ggcgtcacct tgcggcggat gtcgcccagg tcgggcagcc agcccgactg
     2341 ctcgtcgcag acgtagtgca ccggcgtgcc gcccgacagg ccgaccacgg ccgtgtacag
     2401 cgggtagtcc ggcgacggga tcagcacctc gtcgcccgag tcgagcagcg cgttcatgct
     2461 gaacgagatg agctcggagg cgccgttgcc caggtagacg tcgtccaccg tcacgccggc
     2521 gacgcctttt tcctgcgtgt agtgcaccac cgacttgcgc ggcgcgaaca gacccttgga
     2581 atcggtgtag gccgcggcgc tggccaggtt gcggatcatg tcctgcacga tctcgtcggg
     2641 cggctcgagg ccgaacgcgg ccacgttgcc gatgttcagc ttgatgatct tctggccctc
     2701 gtcctccatc tgccgggcct tgtcgaggac cgggccgcgg atgtcgtagc cgatgttggc
     2761 gagcttgctg gacttggcga tcggcttcac ggccggggtt cctgtggcga aggaggaatg
     2821 ggggcggcct ccgggggagg cgccggcgtg tcggtgtcgg cccgagtttc tcatagggaa
     2881 tccccggtcc ggcgcgccgt cgaggacgcg ccgtcgcgcg ggcgggacct cggcaatgcc
     2941 ccgtccgccc cggtttgccg gcgcatccgc cgcgttcgc
//
LOCUS       DATCRO010000725         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_8918, whole genome shotgun sequence.
ACCESSION   DATCRO010000725 DATCRO010000000
VERSION     DATCRO010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8918"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..446)
                     /locus_tag="VIN75_19530"
     CDS             complement(108..446)
                     /locus_tag="VIN75_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976417.1"
                     /translation="MSVDDELSVVVDEVDEFDDEVSLVAGIAAGAALVESFVVVVVVL
                     DVLLGAVLLSVLDGSVVVVVVLLGAAVVSAGWVWVVVVVVVDESFVCATASPIAASRP
                     VAAARTDNFF"
     gene            complement(565..1179)
                     /locus_tag="VIN75_19535"
     CDS             complement(565..1179)
                     /locus_tag="VIN75_19535"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY8976418.1"
                     /translation="MPAPPRRPLCDRCELPLRTCVCALVAHVANEIDVLVLQHPDEAR
                     EAKNSARLLQLSLARCRVVVGEVFEPAALLQLLDGDVSGSALLYPPDTHRGPQVPNSW
                     GLAARPARLVVLDGTWRKSAGMLGANALLQSLPRWPLHPAAPARYGALRKAPRPSQLS
                     TLEATCAALADMEAAPARYAPLLDAFDRFVAAGAARAGRRSTPE"
     gene            1241..1753
                     /locus_tag="VIN75_19540"
     CDS             1241..1753
                     /locus_tag="VIN75_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8976419.1"
                     /translation="MHETHDPTSHGQEKLSELIKDIKFAMFTTHRPNGGHLHSRPMTT
                     LNKHVDDDSLWFFMSRSGDPVAEFEGDDQVNVSYADPGADTYVSVSGIAEVVEDPIKK
                     QALWNKAAQAWFKGGVDDPDLALVRVRIEHADWWDVKENKLVQLYRQAEAALSGKTPK
                     MGEHGRVRPH"
     gene            complement(1803..2573)
                     /locus_tag="VIN75_19545"
     CDS             complement(1803..2573)
                     /locus_tag="VIN75_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8976420.1"
                     /translation="MTTNPSRIALVTGGSRGLGRSAVLKLAERGVDAILTYKTNEAEA
                     HAVVRDVQALGRQAVALQLDAGDSKSFAAFAERVKAALAATWQRPDFDYLVNNAGMGV
                     DHASVAETTEAQFDLLMNVHLKGTFFLTQKLLPLVKDGGRIVNTSSGLARFTLSGYAA
                     YAVMKGGVEVLTRYLAKELGARGIAVNTIAPGAIATDFGGGRVRDNAQLNAFVASQTA
                     LGRAGLPDDIGNAVAALLTEPNGWINAQRIEVSGGMFI"
     gene            2687..3595
                     /locus_tag="VIN75_19550"
     CDS             2687..3595
                     /locus_tag="VIN75_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976421.1"
                     /translation="MDLHELRIFATVAELASFSRAAEQLGLAKGRVSTAVQQLEAQVG
                     TRLLQRTTRRVRLTPDGERFLDRCKELLTEAEQLEAMFRPASTGLTGRLRIDLPTRIA
                     RDLVLPRLPEFLAAHPQLEIGISTTDRLVDLVHEGFDCVLRVGALANSDLVARPLGFL
                     PMRNVASPAYLRAHGTPRTLADLAGHRVVHYMPSLHPQGAAWEYVDDQGQRRTRPMRA
                     QVTVNGTDAYQAAALAGLGLIQAPALGTDALVAGGALVDVLPAYRAPSMPVSLMLPHR
                     RQLAPRVQAVMAWLAALVVPQLSPTP"
     gene            3725..4363
                     /locus_tag="VIN75_19555"
     CDS             3725..4363
                     /locus_tag="VIN75_19555"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY8976422.1"
                     /translation="MRTALTLSLAAAAALFTIHAQAADQTRSVAPFSAVSNSGPINLV
                     IEVGKAQSVVVSGSDDYLEDVRTEVIGDELRIKMHDDSHIDNRHWNDMKVTVTVPKLT
                     AFTMGGAGATTITHVSGDSLDIRFGGAGSLKADGSVKTLRLNVGGVGSIDLHDLHADV
                     ADVKLGGVGSVKVWATQTLDASVGGVGSLTYYGEPKQVNTHGGGLGSISKGK"
BASE COUNT          697 a         1579 c         1523 g          592 t
ORIGIN      
        1 gtttccttac agatgcggca ccgcacttgc tgcaaccctc tccgccagaa ggctcggcgc
       61 tgccgatccg caacacaccc aacctggaga cccctcatgc aaagctatca gaagaagttg
      121 tccgtcctcg ccgccgccac gggcctgctg gccgcgattg ggctggccgt cgcgcagacg
      181 aacgactcgt cgaccaccac gaccacgacg acccagaccc agccggccga cacgaccgcg
      241 gcgccgagca gcacgaccac cacgacgacc gatccgtcga gcacgctcaa cagcaccgcg
      301 ccgagcagca cgtcgagcac gaccacgacg acgacgaacg actcgaccag cgccgcgccc
      361 gccgcgatgc cggccacgag cgacacctcg tcgtcgaact cgtccacctc gtcgaccacg
      421 accgacaact cgtcgtcgac cgacacgacg ccggctcccc gccacgaccg caactgaggc
      481 cgcgcgacct ggtgacccgg gctcccggcc tcgccgggag cgcggagcga tcgccgccgt
      541 gtccgtccgg cacggcggcg atcttcattc cggggtcgat ctccggcctg cgcgggcggc
      601 gccggccgcg acgaaacgat cgaacgcgtc cagcagcggc gcgtagcgcg cgggggcggc
      661 ctccatgtcg gccagcgccg cgcaggtcgc ctccagcgtg ctgagctgcg acggccgcgg
      721 cgccttgcgc aacgcgccgt agcgcgccgg tgcggccgga tggagcggcc atctcggcaa
      781 ggactgcagc aaagcgttcg caccgagcat cccggcgctc ttgcgccacg tgccatcgag
      841 caccacgagc cgcgccggcc gggccgccag gccccaggaa ttggggactt gcgggccccg
      901 atgggtgtca ggcgggtaaa gcagcgcgct gccggataca tcaccgtcga gcaattgcag
      961 cagcgccgca ggctcgaaga cctcgccgac gacgacgcgg cagcgcgcca ggctcagctg
     1021 cagcagccgc gcgctgttct tggcctcgcg cgcctcgtcg ggatgctgca gcacgagcac
     1081 gtcgatctcg ttggccacat gggcgacgag cgcgcacacg caggtgcgca ggggcagttc
     1141 gcagcggtcg cagagcgggc gtcgaggcgg ggcaggcatg gtcgggtggg cgtgagcctt
     1201 gccgctgcgg ggcaaggacg tcacgactgg agatcatccc atgcatgaaa cgcacgaccc
     1261 gacctcgcac ggccaggaga agctctccga gctgatcaag gacatcaagt tcgcgatgtt
     1321 caccacgcac aggcctaatg gcggccacct gcattcgcgg ccgatgacca cgctgaacaa
     1381 gcacgtcgac gacgactcgc tgtggttctt catgtcgcgc tcgggcgacc cggtggccga
     1441 gttcgagggc gacgaccagg tcaacgtgtc gtacgccgac ccgggcgcgg acacctacgt
     1501 ctcggtgtcg ggcatcgccg aggtcgtcga ggacccgatc aagaagcagg cgctgtggaa
     1561 caaggcggcg caggcctggt tcaagggcgg cgtggacgac cccgacctcg cgctcgtgcg
     1621 cgtgcgcatc gagcacgcgg actggtggga cgtgaaggag aacaagctgg tgcagctgta
     1681 caggcaggcc gaggccgcgc tctcgggcaa gacgccgaag atgggcgagc acggccgcgt
     1741 gcgcccgcac tgagcgctgc ggcgcgggcg ccgggcccgc gtcgtcgcac gccaaggcgt
     1801 gttcagatga acatgccgcc ggacacctcg atgcgctgcg cgttgatcca gccgttgggc
     1861 tcggtcagca gcgcggcgac ggcattgccg atgtcgtcgg gcagccccgc gcggccgagc
     1921 gcggtctgcg acgcgacgaa cgcgttgagc tgcgcgttgt cgcgcacgcg gccgccgccg
     1981 aagtcggtgg cgatggcgcc cggggcgatc gtgttgaccg cgatgccgcg cgcgccgagc
     2041 tccttggcca ggtagcgcgt cagcacctcc acgccgccct tcatcacggc gtaggccgcg
     2101 tagcccgaca gcgtgaagcg cgccaggccg ctggaggtgt tgacgatgcg gccgccgtcc
     2161 ttgaccagcg gcagcagttt ctgcgtcagg aagaacgtgc ccttgaggtg gacgttcatc
     2221 agcaggtcga actgcgcctc ggtcgtctcg gccacgctcg cgtggtcgac acccatgccg
     2281 gcgttgttga ccaggtagtc gaagtcgggt cgctgccagg tcgcggccag cgcggccttc
     2341 acgcgctcgg cgaacgcggc gaaggacttg ctgtcgccgg catcgagctg cagggcgacg
     2401 gcctggcggc ccagcgcctg cacgtcgcgc accacggcgt gcgcctcggc ctcgttggtc
     2461 ttgtacgtga ggatcgcgtc gacgccgcgt tcggcgagct tgagcacggc gctgcggccg
     2521 aggccgcggc tgccgccggt gacgagggcg atgcgggagg ggttcgtggt catggcgggc
     2581 tccttggagt gggatggtcg atgagtcgat tttgggaatt cctccatcac ggataaagtc
     2641 cgcatcgacg gccacactgt tccgtcccac ggaacaatcc agagcaatgg acctgcacga
     2701 actgcgcatc ttcgccaccg tggccgagct cgccagcttc agccgcgccg ccgaacagct
     2761 gggcctggcc aagggacgcg tgtcgacggc ggtgcagcag ctcgaggccc aggtcggcac
     2821 gcgcctgctg caacgcacga cgcgccgcgt gcgcctgacg cccgatggcg agcgcttcct
     2881 cgaccgctgc aaggagctgc tgaccgaagc cgagcagctc gaagcgatgt tccgccccgc
     2941 gtccaccggc ctgaccggac gcctgcgcat cgacctgccc acgcgcatcg cgcgcgatct
     3001 cgtgctgccg cggctgcccg agttcctggc cgcgcatccg cagctggaga tcggcatcag
     3061 caccaccgac cgcctggtcg acctcgtgca cgagggtttc gactgcgtgc tgcgcgtcgg
     3121 cgcgctggcc aactccgacc tggtcgcgcg gccgctgggc ttcctgccga tgcgcaacgt
     3181 cgcgagcccg gcctacctgc gcgcgcacgg cacgccccgc acgctcgcgg acctggccgg
     3241 ccatcgcgtc gtccactaca tgccgtcgct gcatccgcag ggcgccgcat gggagtacgt
     3301 cgacgaccag ggccagcgtc gcacgcggcc gatgcgtgcg caggtcaccg tcaacggcac
     3361 cgacgcgtac caggccgcgg cgctggcggg cctggggctg atccaggcac ccgcgctcgg
     3421 caccgacgcg ctggtggccg gcggcgcgct cgtcgacgtg ctgccggcgt accgcgcgcc
     3481 gtccatgccg gtgtcgctga tgctgccgca ccggcgccag ctcgcaccgc gcgtgcaggc
     3541 ggtgatggcg tggctggcgg cgctggtcgt gccgcagctg tcgcccacgc cctgacgcgg
     3601 caatccggtc gcgcgcaggc gcagcgcgtc cggtccagcc gcacctcgtc gcatggcggt
     3661 cgccagggcg ctgacgcgtt cctagaatcg atcgcatcga ctccggcaac acacgaggaa
     3721 cctcatgcgc accgccctga ccctttcgct ggccgccgcc gccgcgctct tcaccatcca
     3781 cgcgcaggcc gccgaccaga cgcgcagcgt cgcgccgttc tcggcggtct cgaactcggg
     3841 gccgatcaac ctggtgatcg aggtgggcaa ggcgcagtcg gtcgtggtct cgggcagcga
     3901 cgactatctc gaggacgtcc gcaccgaggt catcggcgac gagctgcgca tcaagatgca
     3961 cgacgactcg cacatcgaca accggcactg gaacgacatg aaggtcaccg tcacggtgcc
     4021 caagctgacg gccttcacca tgggcggcgc cggcgcgacg acgatcacgc acgtcagcgg
     4081 cgacagcctc gacatccgct tcggcggcgc cggcagcctc aaggccgacg gcagcgtgaa
     4141 gacgctccgg ctcaacgtcg gcggcgtcgg ctcgatcgac ctgcacgacc tgcacgccga
     4201 cgtggccgac gtgaagctcg gcggcgtcgg ctccgtcaag gtctgggcca cccagacact
     4261 ggacgcgagc gtgggcggcg tgggctcgct gacgtactac ggcgaaccca agcaggtcaa
     4321 cacgcacggc ggcgggctgg ggagcatctc gaaaggcaag tgacaccggg attggccgcg
     4381 cggtgcggca g
//
LOCUS       DATCRO010000726         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_132111, whole genome shotgun sequence.
ACCESSION   DATCRO010000726 DATCRO010000000
VERSION     DATCRO010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132111"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VIN75_19560"
     CDS             complement(<1..1745)
                     /locus_tag="VIN75_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8976423.1"
                     /translation="MNILARLNIGQRLVAGFASVLVLAVIVGSFAVSRLARVNDNTTD
                     IATNWLVATRMLGNFGTQVSVLRRAEATSLLSGKPEVIAAQSRRIEATKAKAAESWKA
                     YMATVELPEERAIASGIEAARDRYYVALGHSMAIAADAATPEGREKALAEYEGESKAS
                     FDAMFDGIAHDVEFQGQGADGAYKSSQDTYDHARMAIIGMLAAAVFVGAMMAWVITRS
                     IVRPLASAVKVAEAVAQGNLASTIVANGADEVGQLLRALRRMNDSLVAIVSRVRNSSD
                     SIATGSSQIAAGNTDLSQRTEEQASNLQQTAASMEELTATVKQNADTARAATQIANSA
                     TAAASAGGRVVGQVVTTMDEISQSSRKVVDIIGVIDGIAFQTNILALNAAVEAARAGE
                     QGRGFAVVAGEVRSLAQRSAEAAKEIKTLIGASVEKVESGSRLVDEAGRSMADIVTQV
                     SRVNDLIGEISAASLEQSTGIGQIGDAVNQLDQVTQQNAALVEESAAAAESLKVQAAQ
                     LAQVVSVFKLAAGHEGIAAAPLHAAAARPAPAAQRPGAAAAKAKPPAARSAVKPAPAA
                     PSAPAAAEVGVLAHFP"
     gene            1953..2666
                     /locus_tag="VIN75_19565"
     CDS             1953..2666
                     /locus_tag="VIN75_19565"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY8976424.1"
                     /translation="MPHAVSRLRAERLARSAKPFLARGGSTRERCEACRLPLTHCACA
                     LRPAASAHAGVCLLMADIEPLKPTNTGWLVADVVPDTFAFGWARTQVDPALLALLADP
                     AWQPLVVFPGEYAEPSRVIHDVPRGRGRRPLFVLLDGTWSEARKMFRKSPYLDRLPVL
                     SLRPEQVSSYVLRRSRRDDHFCTSEVASLCLDLAGDARASRALDAWLDVFTHHYLQAR
                     HQLPPACDGEAHLRLRALA"
     gene            complement(3392..>4548)
                     /gene="glpK"
                     /locus_tag="VIN75_19570"
     CDS             complement(3392..>4548)
                     /gene="glpK"
                     /locus_tag="VIN75_19570"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY8976425.1"
                     /translation="REQGLEPVFRAKTGLIVDAYFSGTKLKWILDNVEGTRAAAARGE
                     LAFGTVDTWLMWHLTGGKVHATDVSNASRTLLLDVHANAWSDELLQILDVPRAMLPSV
                     HPSSHVFGHTLPELLGGAIPIGGVAGDQQSALFGQACFRPGLAKNTYGTGCFMLMHTG
                     AAFQNSTNGLITTSAAQLSTAPEFALEGSVFIGGAVVQWLRDGLRAISASGEVQKLAE
                     SVPSSGGVMFVPAFTGLGAPYWDADARGAIVGLTRGSTVAHIARAALESIAFQSAALL
                     QAMSRDAVAAGGAPVAELRVDGGACVNDLLMQFQADLLGIPVVRPRVIETTALGAAYL
                     AGLSCGLYRDVDELAAQWQAERRFLPTMSREQAGTLMADWEHAVRQATAR"
BASE COUNT          596 a         1641 c         1621 g          690 t
ORIGIN      
        1 gggaagtgtg cgagcacgcc gacttcggcg gccgctggcg cgctcggcgc cgccggcgcc
       61 ggcttgacgg cgctgcgcgc agcggggggc ttcgccttgg ctgcagccgc gccgggccgc
      121 tgcgccgcgg gtgccggccg ggcggccgcg gcgtgcaacg gcgcggcggc gatgccctcg
      181 tggcccgcgg ccagcttgaa caccgagacc acctgcgcga gctgcgccgc ctgcaccttc
      241 aggctctcgg ccgcggcggc gctttcctcc accagcgcgg cgttctgctg cgtgacctgg
      301 tcgagctggt tgacggcgtc gccgatctgg ccgatgccgg tgctctgctc caggctggcg
      361 gcgctgatct cgccgatcag gtcgttgacc cggctgacct gcgtgacgat gtcggccatc
      421 gaccggccgg cctcgtcgac gaggcggctg ccgctctcga ccttctcgac gctcgcgccg
      481 atcagcgtct tgatctcctt ggccgcttcg gccgaacgct gcgcgagact gcgcacctcg
      541 ccggcgacga ccgcgaagcc gcggccctgc tcgccggcgc gcgccgcctc gaccgccgcg
      601 ttcagcgcca ggatgttggt ctggaaggcg atgccgtcga tgacgccgat gatgtcgacc
      661 accttgcgcg aagactgcga gatctcgtcc atcgtcgtca ccacctggcc cacgacgcgc
      721 ccgcccgcgc tggcggcggc cgtcgcgctg ttggcgatct gcgtggcggc gcgcgcggtg
      781 tccgcgttct gcttgacggt ggcggtgagc tcctccatcg aggcggccgt ctgctgcagg
      841 ttgctcgcct gttcctcggt gcgctggctc aggtcggtgt tgcccgcggc gatctggctg
      901 gagccggtcg cgatcgagtc ggagctgttg cgcacgcgcg agacgatcgc gaccaggctg
      961 tcgttcatgc ggcgcagcgc gcgcaggagc tggcccacct cgtcggcgcc gttggcgacg
     1021 atcgtcgacg cgaggttgcc ttgggcgacg gcctcggcca ccttcaccgc gctggccagc
     1081 ggccgcacga tcgagcgcgt gatgacccat gccatcatcg cgccgacgaa cacggcggcc
     1141 gcgagcatgc cgatgatggc catgcgcgca tggtcgtagg tgtcctgcga ggacttgtag
     1201 gcgccgtcgg cgccctggcc ctggaactcg acgtcgtggg cgatgccgtc gaacatcgcg
     1261 tcgaagctgg ccttggattc gccctcgtat tcggcgaggg ccttctcgcg gccctcgggc
     1321 gtggcggcgt cggcggcgat cgccatcgag tggccgagcg caacgtagta gcgatcgcgc
     1381 gccgcctcga tcccgctggc gatggcgcgc tcctcgggca gctcgacggt ggccatgtag
     1441 gccttccacg actcggcggc cttggccttg gtggcctcga tgcgccgcga ctgcgccgcg
     1501 atcacctccg gcttgccgga cagcaggctc gtcgcctccg cgcgccggag gacgctcacc
     1561 tgcgtgccga agttgccgag catgcgcgtg gccaccagcc agttcgtcgc gatgtcggtg
     1621 gtgttgtcgt tgacgcgggc caggcggctg accgcgaagc tgccgacgat gaccgccagc
     1681 acgagcacgc tggcgaatcc ggcgacgagg cgctggccga tgttcaggcg ggcaaggatg
     1741 ttcatgatgc ttccctgtgc gttggcggtt ggagcggagc ggcctgcgac aggccgggag
     1801 gatggacgga cccgccaccg cggacgggtg ggcaggttca tcgcagcgta cccaatcggt
     1861 tttcggcatg aaacgaagaa aatgaaatca atttgttcgt ttttctgcgg attcggccgg
     1921 atcaccaccc gccgcacccc gacaatgggc cgatgcccca tgccgtgtcc cgcctgcgcg
     1981 ccgagcgcct cgcccgcagc gccaagccct tcctggcccg cggcgggtcc acgcgcgaac
     2041 gctgcgaggc ctgccggctg ccgctcacgc actgcgcctg cgcgctgcgg ccggccgcgt
     2101 cggcgcacgc cggcgtctgc ctgctgatgg ccgacatcga gccgctcaag ccgaccaaca
     2161 cgggctggct ggtggcggat gtcgtgccgg acaccttcgc tttcggctgg gcgcgcacgc
     2221 aggtcgaccc ggcactcctc gcgctgctgg ccgatcccgc ctggcagccg ctggtcgtgt
     2281 tcccgggcga gtacgccgag ccgtcgcgcg tgatccacga cgtgccgcgc ggccgcggcc
     2341 ggcgcccgct gttcgtgctg ctggacggca cctggagcga ggcgcgcaag atgttccgca
     2401 agagcccgta cctggatcgc ctgccggtgc tgagcctgcg gcccgagcag gtatcgagct
     2461 acgtgctgcg gcgctcccgc cgcgacgacc atttctgcac cagcgaggtg gcctcgctgt
     2521 gcctggacct cgcgggggac gcgcgcgcgt cacgcgcgct cgatgcctgg ctggacgtgt
     2581 tcacgcacca ctacctgcag gcgcggcacc agctgccccc ggcgtgcgac ggcgaggcgc
     2641 acttgcggct gcgggcgctg gcctgacgac gtgcgggtct ggccggcggc tgcaacttgc
     2701 gagtcctgac actgaacgtc ggcgacgccg gcgcacgttc ggtgtcaggt ctcgcatgtc
     2761 gcacgacggg caaggcagga tcgccgccgc ttcggcgtgc gacattcaag accagacacc
     2821 tcggccttcc gcccgcgacg acccgtccaa gccggcccga cgatctccgg cgccccggaa
     2881 cgggcccgag gtgtctggtg ttgcatgttg cgcgccgggc cgcgcacgac aggcccaggc
     2941 gtcgaccgcg caacatgcga gacctgacac cgaacgcccg gcaacgctcg cgcacgttcg
     3001 gtgtcaggtc tcgcatgtcg cacgacgggc gaagcaggat cgccgccgct tcggcgtgcg
     3061 acattcaaga ccagacacct cggccttccg cccgcgacga cccgtccaag ccggcccgac
     3121 gatctgcggc gccccggaac gggcccgagg tgtctggtgt tgcattttgc gcgccgggcg
     3181 gcgcacgaca ggcccaggcg tcgaccgcgc aacatgcgag acctgacacc gaacgccggc
     3241 aacgctcgcg cacgttcggt gtcaggtctc gcatggcgca cgacgggcaa ggcaggatcg
     3301 ccgccgcttc ggcgtgcgac attcaagacc agacacctcg gcctggcgcc cgcgacgacc
     3361 gggtcaagcc gcccggacga tctcccggcc ttcagcgcgc cgtggcctga cgcaccgcat
     3421 gctcccagtc ggccatcagc gtgccggcct gctcgcgcga catcgtgggc aggaaacggc
     3481 gctcggcctg ccattgcgcg gcgagttcgt cgacgtcgcg gtacaggccg caggacaagc
     3541 ccgcgaggta ggcggcgccg agcgccgtcg tctcgatgac gcgcgggcgc accaccggga
     3601 tgcccagcag gtcggcctgg aactgcatca gcaggtcgtt gacgcaggcg ccgccgtcca
     3661 cgcgcagctc ggccaccggt gcgccgccgg cggccaccgc gtcgcggctc atcgcctgca
     3721 gcagcgcggc gctctggaac gcgatgctct cgagcgcggc gcgcgcgatg tgcgccaccg
     3781 tgctgccgcg cgtcaggccg acgatcgcgc cgcgcgcgtc ggcgtcccag tagggcgcgc
     3841 ccaggccggt gaaggccggc acgaacatga cgccgcccga gctgggcacg ctctcggcga
     3901 gcttctgcac ctcgccgctg gcggagatgg cgcgcaggcc gtcgcgcagc cactgcacca
     3961 cggcgccgcc gatgaacacg ctgccttcca gcgcgaattc aggcgccgtg ctcaattgcg
     4021 ccgcgctggt ggtgatcagg ccattggtgg agttctggaa cgcggcgccc gtgtgcatca
     4081 gcatgaagca gccggtgccg taggtgttct tggccagccc gggccggaag caggcctggc
     4141 cgaacagcgc gctctgctgg tcgccggcca cgccgccgat ggggatggcg ccgcccagca
     4201 gctcgggcag cgtgtggccg aacacgtggc tggacggatg caccgacggc agcatcgcgc
     4261 gggggacgtc caggatctgg agcagctcgt cgctccacgc gttggcgtgc acgtccagca
     4321 gcagcgtgcg cgaggcgttg ctgacgtccg tggcgtgcac cttgcccccg gtcaggtgcc
     4381 acatcagcca ggtgtcgacg gtgccgaacg cgagctcgcc gcgtgcggcg gccgcgcgcg
     4441 tgccctcgac gttgtcgagg atccacttga gcttggtgcc cgagaagtag gcgtcgacga
     4501 tcaagcccgt cttcgcccgg aacaccggct ccaggccctg ttcgcgca
//
LOCUS       DATCRO010000727         6884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_188489, whole genome shotgun sequence.
ACCESSION   DATCRO010000727 DATCRO010000000
VERSION     DATCRO010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188489"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VIN75_19575"
     CDS             complement(<1..520)
                     /locus_tag="VIN75_19575"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEY8976426.1"
                     /translation="MNWDDGDGPFFNPQPAVTSVPLPSGKPVVVIDDVLLDPDGLVEW
                     ARARPFHAPDFPYPGLIVDGPKALSERFLDFFAQHARGRLGARRTREHDVRLSMVSTP
                     PSELDPRQWQCHRDRVTTDPSVLFVGSVLYLFRDPALGGTSFYVPRQSPGATDRIVAD
                     SQMLTAEEFSTRY"
     gene            complement(545..3004)
                     /locus_tag="VIN75_19580"
     CDS             complement(545..3004)
                     /locus_tag="VIN75_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8976427.1"
                     /translation="MKFRLARPALALCLVACAAQALAADCRPDPLPGRDLYVRGTPNG
                     WRADDEGAMRWLCDHWEWAGELHGDHAFKIGDEDWSVDADLGVAPGTSALASGASAPT
                     ARKGEPVKHHFDGMSRVTLTPAATPDGAPVLTISDLPAGTPPAAAPPSVTDPLALSVS
                     FDSRKATDKTPFGAVTSGSEVAFSLGALPGVSQVTLVVEKRRLEGDQTTLDYTELARV
                     PLARAAAGADGRERWSGHFRFDAPAIYGYWFELKIGARTFVFENNATPIYWTRERGAG
                     GAGVVADVPHSARRIRRFRQTVYAPYVVPAWARDAVYYYIFPERFRNGDPRNDPKPGV
                     DRYHDKTVEFHANWNDKPWRPHSGDGSDDEYSNDFFGGDIAGIIQKLDYIRSVGANTL
                     YITPMFTAASNHKYDTADWKHIDPHFGTNDDFARLCREAARRGIRVLPDASLNHSGAD
                     SIYFDRYGTRGGHGAFEGGRINPASPWASWYTFDPSQKEPDKQYKGWVGVADLPELNK
                     ADPSYRAFAYGAPDSVTRTWLRAGAAGWRMDVAPWVPDDFWRAWRAVVKETRPDALTV
                     SETWWDASKFFLGDEFDSTMNYIFRNAVLAYANGGDAALMVANLEQMREAYPPQTFAA
                     LMNLLSTHDAARSLHVLGDVDGQSTPEQVALAKQKMRLAVFFQMTYPGSPAIYYGDEV
                     GVTGGDDPYNRGTYPWADKGGHPDDALLADFRRLTALRRALPVLRHGTLLAPLHVDEH
                     VVVLARRDGADWAITASNNGDAPRTLAIALPEGAPTAGWTDVLARTPATVDAHARTLT
                     LVVPPRFGVVVATKPRTTAGR"
     gene            complement(3001..4617)
                     /locus_tag="VIN75_19585"
     CDS             complement(3001..4617)
                     /locus_tag="VIN75_19585"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8976428.1"
                     /translation="MTRPLVTTLAGALCALAALPSIAQQSTYANGFDQALAGVFEARE
                     KDWRDGAVVYQVHVDRFAPPADLQAKRSLYPAPKKLHAWTDLPTHGTYLPEARVWSHE
                     IDFWGGDLQSLRGKLDYVQGLGADVLYLNPIALATTNHKYDTLDYRAVSPEYGTRDDA
                     RALARDVHGRRMKIVLDGVFNHMGRNSPLFRQAESDPRSRYRDWFVFGPQYAGGARSW
                     ALAENLPELNLENPSVRDDLWNAHDSVVRGWLRDGIDGWRLDVAFDIGFKYLGELTQA
                     AHAEKPGSLVVGEIANFPREWFPSVDGLIHFTLRQVILDVAAARIDPATGQQMIARIV
                     QDADYEHVLKSWVYLDNHDTPRLATALPDPRARRLAQVLQFTLPGSPELYYGTELDMP
                     GGDDPEMRATMRWDLVKPDNAVLNWNKRLIALHKDNRALRVGNYRAITAGKLMGFERW
                     TDRAADTIVVLLNPTDQPVTDTVLVANSKLMDGSRMVNLLADKADPATPADVRLSAAL
                     MHLTLPAHGFVVLKPDVAPPGGYTNYKRVQ"
     gene            complement(4614..5504)
                     /gene="malG"
                     /locus_tag="VIN75_19590"
     CDS             complement(4614..5504)
                     /gene="malG"
                     /locus_tag="VIN75_19590"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEY8976429.1"
                     /translation="MAIVTGKNQRWRVLGAHAFLIALIVVTLFPLAAVISISLRPGNF
                     ANGSLIPREISLEHWKLALGMDVTTADGVVVHPPFPVLLWLWNSIKVATIASALIVAI
                     STTAAYAFARLKFAFKLPILNSMLLLQMFPPVLALVAIYAIFETIGNYIPGLGLETHG
                     GLVVAYLGGVATHIWTIRGYFETIPVEIEECAKVDGASHFQAFFRVLLPMAVPILMVV
                     FVLAFVGTIIEYPVASILLREENNLTLAVGSKYYLADQKYLWGDFAAAAVLSGLPITV
                     VFLAAQRWIVSGLTAGGVKE"
     gene            complement(5509..>6884)
                     /gene="malF"
                     /locus_tag="VIN75_19595"
     CDS             complement(5509..>6884)
                     /gene="malF"
                     /locus_tag="VIN75_19595"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEY8976430.1"
                     /translation="RAALAWRYLFPGVVGMLLFVAFPLIYTMWIGFTNYSSIHLLSQA
                     RVKAYLLEQTVPDEDHSLEFTLHADGDKLRVVLAPQDAASGAPKFVSPPLRLKGDDDA
                     TTTMAALPADAPLAPALSTREQLAHRDALQALKLRMPDGRTLVYTGVHEFGPSAAVWR
                     ANPDESITRVDDGKTYKPDTDAGFYVAADGERLQPGFKVAVGFANYARMMADQEFRGP
                     FVSIFVWTVVFAALSVAFSLAVGCTLAVLLNWEALRFRTLYRTLLFLPYAVPGFISIL
                     VFKGLFNQNFGEINGILNAVFGVKPAWFADPLLARTMMLVVNTWLGYPYFLVLCTGLL
                     KAIPVDLYEASAIAGARPLTNFFKITAPLIVKPLSPLIISAFAYNFNNFVLVSLLTNG
                     RPDFLNTKIPAGTTDILVSYTYRIAFLDSGQNFGLAAAISTVIFFLVALLSYANLRVA
                     ARNAKKA"
BASE COUNT         1062 a         2233 c         2557 g         1032 t
ORIGIN      
        1 catagcgcgt cgagaactcc tcggcggtca gcatctggct gtcggccacg atgcggtcgg
       61 tcgcgcccgg cgactggcgc ggcacgtaga agctggtgcc gccgagcgcc ggatcgcgga
      121 acaggtacag caccgagccc acgaacagga cggacgggtc cgtcgtcacc cgatcgcggt
      181 ggcactgcca ctggcgggga tcgagctcgg agggtggcgt gctgaccatc gacagccgca
      241 cgtcgtgctc ccgcgtgcgg cgcgcgccga gacgcccgcg tgcgtgctgc gcgaagaaat
      301 cgaggaagcg ctccgacagc gccttcgggc cgtcgacgat caggccggga tagggaaaat
      361 ccggcgcgtg gaagggccgg gcgcgcgccc attcgacgag accatcggga tcgagcagca
      421 cgtcgtcgat gaccaccacc ggcttgcccg aaggcagcgg cacgctcgtg accgccggtt
      481 gcggattgaa gaaggggccg tcgccgtcgt cccagttcat ggccggcagt ctcgcacgaa
      541 gccgtcagcg gcccgcggtc gtcctcggct tcgtcgcgac caccacgccg aagcgcggcg
      601 gcacgaccag cgtcagcgtg cgggcatggg cgtccaccgt cgcaggcgtc ctggcgagca
      661 cgtcggtcca gcccgcggtc ggcgcgcctt cgggcaacgc gatcgccagc gtgcgcggcg
      721 cgtcgccgtt gttgctcgcg gtgatggccc agtcggcccc gtcgcggcgc gcgagcacga
      781 cgacgtgctc gtcgacgtgc agcggcgcca gcagcgtgcc gtgccgcagc accggcaggg
      841 cgcggcgcaa cgcggtcagg cggcggaagt cggccagcag cgcgtcgtcg ggatggccgc
      901 ccttgtcggc ccaggggtag gtgccgcggt tgtacggatc gtcgccgccc gtcacgccca
      961 cctcgtcgcc gtagtagatc gcaggcgagc ccggatacgt catctggaag aacacggcca
     1021 ggcgcatctt ctgcttggcg agcgcgacct gttcgggcgt gctctggccg tcgacgtcgc
     1081 ccagcacgtg cagcgagcgg gcggcgtcgt gcgtggacag caggttcatc agcgcggcga
     1141 aggtctgcgg cggataggcc tcgcgcatct gctcgaggtt ggccaccatc agcgccgcgt
     1201 cgccgccgtt ggcgtaggcc aggacggcgt tgcggaagat gtagttcatc gtcgagtcga
     1261 actcgtcgcc gaggaagaac ttggacgcgt cccaccaggt ctcggacacg gtgagcgcgt
     1321 cgggccgggt ctccttgacc accgcgcgcc aggcgcgcca gaagtcgtcg ggcacccagg
     1381 gggcgacatc catgcgccag ccggcggcgc cggcgcgcag ccatgtgcgg gtgacggagt
     1441 cgggcgcgcc gtacgcgaac gcgcggtacg acgggtcggc cttgttcagc tcgggcaggt
     1501 cggccacgcc cacccagccc ttgtactgct tgtccggctc cttttgcgat ggatcgaacg
     1561 tgtaccagct ggcccagggc gacgccggat tgatgcggcc gccctcgaac gcgccgtggc
     1621 cgccgcgcgt gccatagcgg tcgaagtaga tcgagtcggc gcccgagtgg ttcagcgacg
     1681 cgtcgggcag cacgcggatg ccgcgtctgg ccgcctcgcg gcacaggcgc gcgaagtcgt
     1741 cgttcgtgcc gaagtgcggg tcgatgtgct tccagtcggc ggtgtcgtac ttgtggttgc
     1801 tggccgcggt gaacatgggc gtgatgtaca gcgtgttcgc gccgacgctc ctgatgtagt
     1861 cgagcttctg gatgatgccg gcgatgtcgc cgccgaagaa gtcgttgctg tactcgtcgt
     1921 cgctgccgtc gccgctgtgc gggcgccagg gcttgtcgtt ccagttcgcg tggaactcca
     1981 ccgtcttgtc gtggtacctg tcgacgccgg gcttggggtc gttgcgcgga tcgccgttgc
     2041 ggaagcgctc ggggaagatg tagtagtaca cggcgtccct ggcccacgcg ggcaccacgt
     2101 agggcgcgta aacggtctgg cggaagcggc ggatgcgcct ggccgagtgc ggcacgtcgg
     2161 cgacgacgcc cgcgccgccg gcaccgcgtt cgcgcgtcca gtagatgggc gtcgcgttgt
     2221 tctcgaagac gaaggtgcgc gcgccgatct tcagctcgaa ccagtagccg tagatggccg
     2281 gtgcatcgaa gcggaagtgg ccgctccagc gctcgcgccc gtctgcgccc gcggccgcgc
     2341 gcgcgagcgg cacgcgcgcg agctcggtgt agtcgagcgt cgtctggtcg ccctcgaggc
     2401 ggcgcttctc gacgacgagg gtcacctgcg acacgcccgg cagggcgccc agcgagaacg
     2461 cgacctcgct gccgctggtg accgcgccga agggcgtctt gtcggtggcc ttgcgcgaat
     2521 cgaaggacac gctcagcgca agcgggtcgg tgacggacgg cggcgcggcc gcaggcggcg
     2581 tgccggcggg caggtcgctg atcgtgagca ccggcgcgcc gtcgggcgtc gcggcgggcg
     2641 tcagcgtcac gcggctcatg ccgtcgaagt ggtgcttgac cggctcgccc ttgcgcgccg
     2701 tgggcgcgct cgcgccggag gccagcgccg acgtgcccgg cgcgacgccg agatccgcgt
     2761 cgacgctcca gtcctcgtcg ccgatcttga acgcgtggtc gccatgcagt tcgccggccc
     2821 actcccaatg gtcgcacagc cagcgcatcg cgccttcgtc atcggcgcgc cagccgttgg
     2881 gggtgccgcg cacgtagagg tcgcgtccgg gcagcgggtc ggggcggcag tcggcggcca
     2941 gggcctgcgc ggcgcacgcg acgaggcaca gcgcgagcgc ggggcgggcg agtcggaact
     3001 tcattggacg cgtttgtagt tggtgtagcc gcccggcggc gcgacgtcgg gcttgaggac
     3061 gacgaacccg tgcgcgggca gcgtgaggtg catcagcgcg gcggacaggc ggacgtcggc
     3121 gggcgtggcc gggtcggcct tgtcggccag caggttgacc atgcgcgaac cgtccatcag
     3181 cttggagttg gccacgagca cggtgtcggt gacgggctgg tcggtcgggt tcagcagcac
     3241 gacgatggtg tcggccgcac ggtcggtcca ccgctcgaag cccatcagct tgccggcggt
     3301 gatcgcgcgg tagttgccga cgcgcagcgc gcggttgtcc ttgtgcagcg cgatcagccg
     3361 cttgttccag ttgagcacgg cgttgtcggg cttgacgaga tcccagcgca tggtcgcgcg
     3421 catctcgggg tcgtcgccgc cgggcatgtc cagctcggtg ccgtagtaca gctccggcga
     3481 gccgggcagc gtgaactgca gcacctgcgc gaggcggcgc gcgcgcgggt cgggcagggc
     3541 ggtggccagg cgcggcgtgt cgtggttgtc gagatagacc caggacttca gcacgtgctc
     3601 gtagtcggcg tcctgcacga tgcgcgcgat catctgctgg ccggtggccg ggtcgatgcg
     3661 ggccgccgcc acgtcgagga tcacctggcg cagcgtgaag tggatcaggc cgtcgaccga
     3721 cgggaaccat tccctcggga agttggcgat ctcgccgacc accagcgagc cgggcttttc
     3781 cgcgtgcgcg gcctgcgtca gctcgccgag gtacttgaag ccgatgtcga aggcgacatc
     3841 caggcgccag ccgtcgatgc cgtcgcgcag ccagccgcgc accacggagt cgtgggcgtt
     3901 ccacaggtcg tcgcgcaccg acggattctc gagattgagc tcgggcaggt tctcggccag
     3961 cgcccaggag cgcgcgccgc cggcgtactg cgggccgaac acgaaccagt cgcgatagcg
     4021 gctcctgggg tcggactcgg cctgcctgaa caagggcgag ttgcggccca tgtggttgaa
     4081 gaccccgtcc agcacgatct tcatgcggcg cccgtgcacg tcacgcgcga gggcgcgggc
     4141 gtcgtcgcgc gtgccgtact cgggcgagac ggcgcggtag tcgagcgtgt cgtacttgtg
     4201 gttggtggtc gcgagggcga tcgggttcag gtagaggaca tcggcgccca gcccctggac
     4261 gtagtcgagc ttgccgcgca ggctctgcag gtcgccgccc cagaagtcga tctcgtggct
     4321 ccagaccctg gcctcgggca ggtacgtgcc gtgcgtgggc aggtcggtcc aggcgtgcag
     4381 cttcttgggc gcagggtaca gcgaacgctt ggcctggagg tcggccggcg gcgcgaagcg
     4441 gtcgacgtgg acctggtaca cgaccgcgcc gtcgcgccag tccttctcgc gcgcctcgaa
     4501 cacgccggcg agcgcctggt cgaagccgtt ggcgtaggtc gactgctgcg cgatcgaggg
     4561 gagcgcggcc aacgcgcaca gcgcgcccgc caaggtggtg acgagcggcc gggtcattcc
     4621 ttgacgcccc cggcagtgag ccccgaaacg atccagcgct gcgccgcgag gaacaccacc
     4681 gtgatcggca ggcccgacag gaccgccgcg gcggcgaagt cgccccacag gtacttctgg
     4741 tcggccaggt agtacttgga gccgacggcc agcgtcaggt tgttctcctc gcgcagcagg
     4801 atcgacgcga cggggtactc gatgatggtg cccacgaagg ccagcacgaa cacgaccatc
     4861 aggatgggca ccgccatcgg cagcagcacg cggaagaagg cctggaagtg gctggcgccg
     4921 tcgaccttgg cgcattcctc gatctcgacg gggatcgtct cgaagtagcc gcggatcgtc
     4981 cagatgtgcg tcgccacgcc gcccaggtag gccaccacga ggccgccgtg cgtctccagg
     5041 ccgaggccgg ggatgtagtt gccgatggtc tcgaagatcg cgtagatcgc caccagcgcc
     5101 agcaccggcg ggaacatctg cagcagcagc atgctgttga ggatgggcag cttgaatgcg
     5161 aacttcaggc gcgcgaacgc gtaggccgcg gtggtcgaga tcgccacgat cagggccgac
     5221 gcgatggtgg ccaccttgat cgagttccac agccacagca gcacggggaa gggcgggtgc
     5281 acgaccacgc cgtcggcggt ggtgacgtcc atgccgagcg ccagcttcca gtgctcgagg
     5341 ctgatctcgc gcgggatcag cgagccgttg gcgaagttgc cgggtcgcag cgagatcgag
     5401 atcacggcgg cgagcgggaa cagcgtgacg acgatcagcg cgatgaggaa cgcatgcgcg
     5461 ccgaggacgc gccagcgctg gttcttgccg gtgacgatgg ccatggcatc aagccttttt
     5521 agcgttgcgc gcggcgacgc gcaggtttgc gtacgacagc agcgcgacca ggaagaagat
     5581 caccgtcgag atcgcggcgg ccaggccgaa gttctggccc gagtcgagga aggcgatgcg
     5641 gtaggtgtac gacacgagga tgtcggtggt gcccgccggg atcttcgtgt tgaggaagtc
     5701 gggccggcca ttggtcagca gcgagaccag cacgaagttg ttgaagttgt aggcgaaggc
     5761 cgagatgatc agcggcgaca gcggcttgac gatcagcggc gccgtgatct tgaagaagtt
     5821 cgtcagtggc ctggcgcccg cgatggccga ggcctcgtac aggtcgaccg ggatcgcctt
     5881 gagcaggccc gtgcacagca ccaggaagta cggatacccg agccaggtat tgacgacgag
     5941 catcatcgtg cgcgccagca gcgggtcggc gaaccaggcc ggcttgacgc cgaagacggc
     6001 gttgaggatg ccgttgatct cgccgaagtt ctggttgaac aggcccttga acacgaggat
     6061 ggagatgaag ccgggcaccg cgtacggcag gaacagcagc gtgcggtaca gcgtgcgaaa
     6121 gcgcagtgcc tcccagttca gcagcaccgc cagcgtgcag cccacggcca gcgagaacgc
     6181 caccgacagc gccgcgaaca cgaccgtcca cacgaagatc gagacgaacg ggccgcggaa
     6241 ctcctggtcg gccatcatgc gcgcgtagtt cgcgaagccg accgccacct tgaagccggg
     6301 ctgcaggcgc tcgccgtcgg cggcgacgta gaagccggcg tcggtgtcgg gcttgtacgt
     6361 cttgccgtcg tccacgcgcg tgatcgattc gtcgggattg gcgcgccaga cggcggccga
     6421 ggggccgaat tcgtggaccc cggtgtagac cagcgtgcgg ccgtcgggca tccggagctt
     6481 gagcgcttgc agcgcgtcgc ggtgggcgag ctgctcgcgc gtggacagcg ccggcgccag
     6541 cggcgcatcg gcgggcaggg cggccatcgt cgtcgtcgcg tcgtcgtcgc ccttcagcct
     6601 caatggcggc gaaacgaact tcggcgcgcc cgacgccgcg tcctgcggcg ccagcaccac
     6661 gcgcagcttg tcgccgtccg catgcagcgt gaattcgagc gaatgatcct cgtccggcac
     6721 cgtctgctcc agcaggtagg ccttcacacg cgcctggctc agcaggtgga tggacgagta
     6781 gttcgtgaag ccgatccaca tcgtgtagat gagcgggaag gccacgaaca gcaacatgcc
     6841 cacgacgccc gggaacaggt agcgccaggc cagcgcggcg cgcg
//
LOCUS       DATCRO010000728         7482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_177025, whole genome shotgun sequence.
ACCESSION   DATCRO010000728 DATCRO010000000
VERSION     DATCRO010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177025"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VIN75_19600"
     CDS             <1..931
                     /locus_tag="VIN75_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY8976431.1"
                     /translation="PWVQRGLVAHWPMVQAARRSTADAMAYLKGTWGGEPVGMLLAPP
                     QTHGRFFYNDAFTGMNFHREKAPLDVVLDALARMAGDPQAPALYVGSTTVDTCLPTFR
                     AANDLPFGDRDPLVSIWLGNRSRIAAHFDLPDNLACVVAGRRRFTLFPPEQLPNLYVG
                     PLDVTPAGQSVSLVDFANPDLERFPRFAEALKHAQVVELEPGDAVFVPSMWWHHVESL
                     TPFNVLINYWWRQSPDWMDSPIGALMAALLTMRDLPPAQREIWRGVFDHYVFRADDAT
                     AAHIPPHARSALAPLTESGARHLRQALLQRFKR"
     gene            939..2474
                     /locus_tag="VIN75_19605"
     CDS             939..2474
                     /locus_tag="VIN75_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8976432.1"
                     /translation="MTMSPTDRSRQPVRRVVIAGGGTAGWMTAACLSKTLGKGLDIQL
                     VESDEIGTVGVGEATIPTLLTFNNLCGINEQEFMAETQGVFKLGISFEGWRDVGEDYF
                     HSFGTTGQDHWTAGFQHFWLKGRDRKLARTYGEYCLELRAAERNRFAHLPRSALNYAF
                     HIDATLYAKYLRKLSERQGARRIEGKIAQVIRHADGDIAALKLADGSVLEGDLFVDCT
                     GMRSLLLGETLGVPLQDWSHWLPCDRAVALQTASVKAPVPYVRSIAHPFGWQWQIPLQ
                     HRVGNGMVYSSQHVSDDQALATLQASIQGEVLTKPRVIPFKPCQRASTWERNVVAIGL
                     SSGFLEPLESTSIHLIQRGIIRLLQCFPNEGVRQADVDEYNRQTRDEMEHIRDFIILH
                     YKVTNRSDSAFWNQVRTMEVPASLQHRIDLFRQSGRVFRAPNELFAENSWIQVMLGQG
                     IMPAQHHPIANLMSDAELNAFLEEIRGQVERTVAQLPAHADYVARYCKAAAPPVRPMP
                     AAA"
     gene            complement(2544..3575)
                     /locus_tag="VIN75_19610"
     CDS             complement(2544..3575)
                     /locus_tag="VIN75_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8976433.1"
                     /translation="MSADVSTAALDRILAEVAATQRRWLVTGAAGFIGSHLLETLLAA
                     GQHVVTLDNFATGHQRNFDKVRASVGEQAWARHVAITGDIADEATCRTACEDVDVVLH
                     QAALGSVPRSLADPLTTHRANATGFLTMLVAARDAKVQRFVYAASSSTYGDSPTLPKV
                     EDVIGRPLSPYAVTKYLDELYADVFCRSYGMPTIGLRYFNVFGPRQDPNGAYAAVIPR
                     WIAAMLEGRECVINGDGETSRDFCFVRNVVQANLRAALTTDAEAVGRVYNVAVGQRTT
                     LNDLHAALATAVEARQPGTRVPAPTRAPFRAGDVRHSLADISLARRLLGYEPTHTIAA
                     GMAETVAAY"
     gene            complement(3572..4858)
                     /locus_tag="VIN75_19615"
     CDS             complement(3572..4858)
                     /locus_tag="VIN75_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY8976434.1"
                     /translation="MNKTLTVAVVGLGYVGLPLAVAFGKKFPTIGFDLSESKIESYRR
                     NIDPMGEIESADLQAARLKLSTDASVLREADFVVIAVPTPVDDAHIPDFSPLVGASTV
                     AGKNLKPGAVVVFESTVYPGATEEVCIPLLEKHSGLKWKKDFFVGYSPERINPGDKEH
                     TLTRIVKVVSGDTPATLEIVADTYGAVIEAGVHRASSIKVAEAAKVIENTQRDLNIAL
                     VNELALIFHRIGIDTTEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAEVLGLH
                     PEVILAGRRINDGMGRYIAEQTVKCMIRRGHAVKDADVLVLGLTFKENCADLRNSKVI
                     DVIRELQSYGARVHVHDPIASPEEALHEYGVHLVKWDDLPRASAIVGAVAHHELVRKS
                     PADLLAKLTPQGVYVDVKCKADQAALTELGVEVWRL"
     gene            5334..6719
                     /locus_tag="VIN75_19620"
     CDS             5334..6719
                     /locus_tag="VIN75_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEY8976435.1"
                     /translation="MFAAFRNVPLSSLFFDVTLCFLAVVLASTLTPNLVGSSNLLPLN
                     WLLTLAAAFSLTAVFMFACLGLYRPVRLRLSATFWRMLTASAIAGCLAYPIFEATLGS
                     NYATLFLPRAIWCLAGCLLVGKYFGRRLSKALRVPRVLIVGTGADAAMVARDFHESKT
                     MQRNVVGYVATPQDEAALPAELGKAQVFDGASSIVDLVHTHGIDEIVVAVRDQRGGNV
                     PMDQLLACRIRGIPVLDLAGCFERARAEVPLDSLKASWLVYGKGFEQGKVRHVVKRLV
                     DIIGSGILLTIAAPIMVLAAVAVHLTSPGPILYRQERVGFGGKIFMCLKFRSMRNDAE
                     KDGVARWATKNDSRVTPVGAFMRKTRIDELPQLFSVLKGEMSLIGPRPERPAFVAELR
                     KHIPFYDLRHSIKPGLTGWAQVRSGYGASIEDARRKHQFDLYYVKNNSLVLDLLVLIE
                     TVSVVVFREGQ"
     gene            6725..7342
                     /locus_tag="VIN75_19625"
     CDS             6725..7342
                     /locus_tag="VIN75_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY8976436.1"
                     /translation="MSAFASVARQLRALFVLALGAGALAGCVATHPPVPAAAASAEDV
                     YKVGPLDSLNIVVWRNTDLSGTVQVRPDGRISTPLVQDLDVAGKTPPEIAREIEKALG
                     KYVRDPNVTVLVTSFQGTFSEQIRIVGEAAKPQAIAYRQNMTLLDVMIQAGGLTDFAD
                     GNSAVLVRNNTSYTVRLKDLLKRGDISANAAVAPGDVIIVPQSWF"
BASE COUNT         1146 a         2616 c         2529 g         1191 t
ORIGIN      
        1 gccctgggtg cagcgcggcc tcgtcgcgca ctggccgatg gtgcaggccg cgcgccgctc
       61 caccgcggac gcgatggcct acctcaaggg cacgtggggc ggcgaacccg tgggcatgct
      121 gctcgcaccg ccgcagaccc acggccgatt cttctacaac gacgccttca cgggcatgaa
      181 cttccaccgc gagaaggcgc cgctggacgt cgtgctggac gcgctggccc ggatggccgg
      241 cgacccgcag gcgccggcgc tgtacgtcgg ctcgaccacc gtcgacacct gcctgccgac
      301 gtttcgcgcc gccaacgacc tgccgttcgg cgatcgcgat ccgctcgtga gcatctggct
      361 gggcaaccgc agccgcatcg ccgcgcactt cgatttgccg gacaacctcg cctgcgtcgt
      421 cgccggccgc cgccgcttca cgctgttccc gccggagcag ttgcccaacc tctacgtcgg
      481 tccgctggac gtgacgcccg ccggccagtc cgtgagcctg gtcgacttcg ccaaccccga
      541 tctcgagcgc ttcccgcgct tcgccgaggc gctcaagcac gcgcaggtcg tcgagctcga
      601 acccggcgac gccgtgttcg tgcccagcat gtggtggcac cacgtcgaat cgctgacgcc
      661 cttcaacgtg ctgatcaact actggtggcg ccagtcgccc gactggatgg actcgccgat
      721 cggcgcgctg atggccgcgc tgctgacgat gcgcgacctg ccgcccgcac agcgcgagat
      781 ctggcgcggc gtgttcgatc actacgtctt ccgcgccgac gacgccaccg ccgcccacat
      841 cccgccgcac gcgcgcagcg cgctcgcccc gctcaccgag agcggcgcgc gccacctgcg
      901 ccaggccctg ctccaacgct tcaaacgctg aggccgccat gaccatgtcc cccaccgacc
      961 gctcccgcca acccgtccgc cgcgtcgtca tcgccggcgg cggcaccgcc ggctggatga
     1021 cggccgcctg cctgtccaag acgctcggca agggcctgga catccagctc gtggagtccg
     1081 acgagatcgg caccgtcggc gtcggcgaag ccaccatccc gacgctgctg acgttcaaca
     1141 acctgtgcgg catcaacgag caggagttca tggccgagac gcagggcgtc ttcaagctgg
     1201 gcatcagctt cgagggctgg cgcgacgtcg gcgaggacta tttccacagc ttcggcacga
     1261 ccggccagga ccactggacg gccggcttcc agcacttctg gctcaagggc cgcgaccgca
     1321 agctcgcgcg cacctacggc gagtactgcc tggagctgcg cgccgccgaa cggaaccgct
     1381 tcgcgcacct gccgcgctcg gcgctgaact acgccttcca catcgatgcc acgctgtacg
     1441 cgaagtacct gcgcaagctc agcgaacgcc agggcgcgcg ccgcatcgag ggcaagatcg
     1501 cccaggtgat ccgccacgcc gacggcgaca tcgccgcgct caagctggcc gacggctcgg
     1561 tcctggaggg cgacctgttc gtcgactgca ccggcatgcg ctcgctgctt ctgggcgaga
     1621 cgctgggcgt gccgctgcag gactggagcc actggctgcc gtgcgaccgc gccgtcgcgc
     1681 tgcagaccgc gtcggtcaag gcgccggtgc cctacgtgcg atcgatcgcg cacccgttcg
     1741 gctggcaatg gcagatcccg ctgcagcacc gcgtgggcaa cgggatggtc tacagcagcc
     1801 agcatgtcag cgacgaccag gcgctggcca cgctgcaggc gagcatccag ggcgaggtgc
     1861 tgaccaagcc gcgcgtcatc ccgttcaagc cgtgccagcg cgcgtccacg tgggagcgca
     1921 acgtcgtggc gatcgggctg tccagtggct tcctcgagcc actggagtcc accagcatcc
     1981 acctgatcca gcgcggcatc atccggctgc tgcagtgctt cccgaacgag ggcgtgcgcc
     2041 aggccgacgt cgacgagtac aaccggcaga cgcgcgacga gatggaacac atccgcgact
     2101 tcatcatcct gcactacaag gtcaccaacc gcagcgacag cgcgttctgg aaccaggtgc
     2161 gcacgatgga ggtgccggcg tcgctgcagc accgcatcga cctgttccgc cagagcggcc
     2221 gcgtgttccg cgcgcccaac gagctgttcg ccgagaactc gtggatccag gtgatgctgg
     2281 gccagggcat catgcccgcg cagcaccacc cgatcgccaa cctgatgagc gatgcggagc
     2341 tgaacgcctt cctggaggag atccgcgggc aggtggaacg caccgtggcg cagctgcccg
     2401 cgcacgccga ctacgtcgcg cgctactgca aggcggcggc gccgccggtc aggccgatgc
     2461 cggcggccgc ttgaggaagt ggcgcgccgt ccgtcgcatg cgcgcgtgac gcgcaggcgc
     2521 gcaggcccgg gcgggcgggc gcctcagtac gcggcgaccg tctccgccat gccggcggcg
     2581 atggtgtgcg tcggctcgta gcccagcagg cgccgcgcca gcgagatgtc ggccagcgaa
     2641 tggcggacgt cgcccgcgcg gaagggcgcg cgcgtgggtg cgggcacgcg cgtgccgggc
     2701 tggcgcgcct cgacggcggt ggccagcgcg gcgtgcaggt cgttcagcgt ggtgcgctgg
     2761 ccgacggcga cgttgtagac gcggccgacg gcctcggcgt cggtcgtcag cgccgcgcgc
     2821 aggttggcct ggacgacatt gcgcacgaag cagaagtcgc gcgaggtctc gccgtcgccg
     2881 ttgatgacgc actcgcggcc ttccagcatc gcggcgatcc agcgcgggat caccgcggcg
     2941 taggcaccgt tcggatcctg gcgcgggccg aagacgttga agtagcgcag gccgatcgtg
     3001 ggcatgccgt agctgcggca gaagacgtcg gcgtacagct cgtcgaggta cttggtgacg
     3061 gcgtacggcg acagcgggcg gccgatgacg tcctcgacct tgggcagggt cggggaatcg
     3121 ccgtacgtgg agctggacgc cgcgtagacg aagcgctgca ccttggcgtc gcgcgcggcc
     3181 accagcatcg tcaggaagcc ggtggcgttg gcgcggtggg tggtcaacgg atcggccagc
     3241 gagcgcggca ccgagccgag cgcggcctgg tgcagcacga cgtcgacgtc ctcgcaggcg
     3301 gtgcggcagg tggcttcgtc ggcgatgtcg ccggtgatgg ccacatgccg ggcccaggcc
     3361 tgctcgccga ccgacgcgcg caccttgtcg aagttgcgct ggtggccggt ggcgaagttg
     3421 tcgagcgtga cgacgtgctg gccggcggcc agcagcgtct ccagcaggtg cgagccgatg
     3481 aagcccgcgg cgccggtgac gagccagcgc ctctgcgtgg cggcgacttc cgcgaggatc
     3541 cggtcgaggg ccgccgtgct gacgtccgcg ctcacaggcg ccagacttcc acgccgagct
     3601 cggtcagcgc agcctggtcg gccttgcact tcacgtcgac gtagacgcct tgcggcgtca
     3661 gcttggcgag cagatcggcc ggcgacttcc tgaccagctc gtggtgcgcc acggcgccga
     3721 cgatggcgct cgcgcgcggc aggtcgtccc acttgaccag gtggacgccg tactcgtgca
     3781 acgcctcctc gggcgaggcg atcgggtcgt gcacgtgcac gcgcgcgccg tagctctgga
     3841 gctcgcggat cacgtcgatc accttggagt tgcgcaggtc ggcgcagttt tccttgaagg
     3901 tcaggcccag caccagcacg tcggcgtcct tgacggcgtg gccgcggcgg atcatgcact
     3961 tgacggtctg ctcggcgatg tagcggccca tgccgtcgtt gatgcggcgg ccggcgagga
     4021 tgacctcggg gtgcaggccc agcacctcgg ccttgtgcgt gaggtagtac ggatcgacgc
     4081 cgatgcagtg gccgccgacc aagcccggac ggaacggcag gaagttccac ttcgtgccgg
     4141 cggcctcgag cacctcggtg gtgtcgatgc cgatgcggtg gaagatcagc gcgagttcgt
     4201 tgaccagcgc gatgttcagg tcgcgctgcg tgttctcgat caccttggcg gcctcggcca
     4261 ccttgatgct gctggcgcga tgcacgccgg cctcgatcac ggcaccgtag gtgtcggcca
     4321 cgatctccag cgtcgccggg gtgtcgccgg acaccacctt gacgatgcgg gtgagggtgt
     4381 gctccttgtc gcccgggttg atgcgttcgg gcgagtagcc cacgaagaag tccttcttcc
     4441 acttcaggcc ggagtgcttc tccagcagcg ggatgcagac ctcttcggtc gcgcccgggt
     4501 agaccgtcga ctcgaacacc acgacggcgc cgggcttcag gttcttgccc gcgaccgtgc
     4561 tcgcgccgac cagcggcgag aaatcgggga tatgggcgtc gtcgaccggc gtgggcacgg
     4621 cgatgacgac gaagtcggcc tcgcgcagca cggatgcatc ggtggacagc ttcaggcgcg
     4681 cggcctgcag gtccgccgac tcgatctcgc ccatcggatc gatgttgcgg cgatagctct
     4741 cgatcttgct ttccgacagg tcgaaaccga tcgtcggaaa cttttttcca aacgcgaccg
     4801 ccagcggcag tccaacgtaa cccaggccga cgacggccac cgtcaaagtc ttgttcattg
     4861 cgtcaatttc cctcccctgc gagtttacgc gagcaacccc cggggccatc ccaccatttt
     4921 tgggggaagt gcgcgaaaca tttgctgaca ctgttgcaat ccgccgtcca tccggccgga
     4981 agacacgcca tcgtcacggt ccggacgaac acttgcagcc gtcaagcgat ccgtcagacg
     5041 aggtagggtc aaccgtgaaa tagttccggt tcgggtagca cgagcccccc caaatgcggg
     5101 gattctcccc cgggtcaagt tattcgatac tggttgcaac agattcggcg cctgcccaca
     5161 caccggccca cgcgggacca tgaggcggtg acagcagtcg gcgggttgtt cggtcagagc
     5221 cctgcgctct acgacacatc ctgtttcatt tgtatcagta gcgtcgacac gcaacgagga
     5281 acggcagacg cagatccgcc gcccgtcgcg agtggcgatg gagggtgctt tccatgttcg
     5341 ctgcattccg caacgtaccg ctgtcgtcgt tgttcttcga cgtcacgctg tgcttcctgg
     5401 ccgtcgtcct ggcgtcgacc ctgacgccga acctggtcgg ctcgtccaac ctgctgccgc
     5461 tgaactggct gctgacgctg gcggccgcct tctcgctgac cgcggtgttc atgttcgcct
     5521 gcctgggcct gtaccggccg gtgcgcctgc gcctgtcggc cacgttctgg cgcatgctga
     5581 ccgccagcgc gatcgccggc tgcctggcct acccgatctt cgaagccacc ctcggctcga
     5641 actacgccac gctgttcctg ccgcgcgcca tctggtgcct ggccggctgc ctgctggtgg
     5701 gcaagtactt cggccgccgc ctctccaagg cgctgcgcgt gccgcgcgtg ctgatcgtgg
     5761 gcaccggcgc cgatgcggcg atggtggcgc gcgacttcca cgaatccaag acgatgcagc
     5821 gcaacgtcgt cggctatgtc gccacgccgc aggacgaggc ggcgctgccg gccgaactgg
     5881 gcaaggcgca ggtcttcgac ggcgcgagct cgatcgtcga cctggtgcac acccacggga
     5941 tcgacgagat cgtcgtggcc gttcgcgacc agcgcggcgg caacgtgccg atggaccagt
     6001 tgctcgcctg ccgcatccgc ggcatccccg tgctcgacct ggccggctgc ttcgagcgcg
     6061 cgcgcgccga ggtgccgctg gacagcctga aggccagctg gctggtctac ggcaagggct
     6121 tcgagcaggg caaggtgcgt cacgtcgtca agcgcctggt cgacatcatc ggctcgggca
     6181 tcctgctgac gatcgccgcg ccgatcatgg tgctggccgc cgtcgccgtg cacctgacca
     6241 gccccggccc gatcctgtac cgccaggagc gcgtgggctt cggcggcaag atcttcatgt
     6301 gcctgaagtt ccgcagcatg cgcaatgacg ccgagaagga cggcgtggcg cgctgggcga
     6361 ccaagaacga cagccgcgtc acgccggtcg gcgccttcat gcgcaagacc cgcatcgacg
     6421 agctgccgca gctgttcagc gtgctcaagg gcgagatgag cctgatcggc ccgcggcccg
     6481 agcgtcccgc gttcgtggcc gaactgcgca agcacatccc gttctatgat ctgcgccaca
     6541 gcatcaagcc cgggctgacc ggctgggccc aggtgcgttc gggctacggc gcgtcgatcg
     6601 aggatgcacg ccgcaagcac caattcgacc tttactacgt gaagaacaac tcgctggtgc
     6661 tcgacctgct cgtcctgatc gagaccgtca gcgtggtcgt cttccgcgaa gggcagtgat
     6721 catcatgagc gcattcgcct cggtcgcccg gcaactccgg gcgcttttcg ttctggccct
     6781 cggcgccggc gcgctcgccg gctgcgtggc cacccacccg ccggtgccgg cggccgccgc
     6841 ttcggcggag gacgtctaca aggtcggccc gctcgattcg ctcaacatcg tcgtctggcg
     6901 caacaccgac ctgtccggca cggtgcaggt gcgcccggac ggccgcatct ccacgccgct
     6961 ggtgcaggac ctggacgtcg cgggcaagac gccgcccgag atcgcgcgcg agatcgagaa
     7021 ggcgctgggc aagtacgtgc gcgacccgaa cgtcacggtg ctcgtcacgt cgttccaggg
     7081 cacgttctcc gagcagatcc gcatcgtcgg cgaggcggcc aagccgcagg ccatcgccta
     7141 ccgccagaac atgaccctgc tggacgtcat gatccaggcc ggcggcctga ccgatttcgc
     7201 ggacggcaac agcgccgtcc tggtccgcaa caacacgtcc tacaccgtgc gcctgaagga
     7261 tctcctcaag cgcggcgaca tcagcgcgaa cgccgcggtc gcgccgggcg acgtcatcat
     7321 cgtgccgcaa agctggttct gaggaaccgg ggatgcgcgt gcgtgcaggg ttgggtggac
     7381 gcccgtgaac cagattttcg aagaagtcct gatcgccttg cgcgcgatct ggcgccgccg
     7441 ctggctcgcc atcgccgcca tgtggagcgt gacgctcgtg gg
//
LOCUS       DATCRO010000729         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_874252, whole genome shotgun sequence.
ACCESSION   DATCRO010000729 DATCRO010000000
VERSION     DATCRO010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874252"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VIN75_19630"
     CDS             complement(<1..223)
                     /locus_tag="VIN75_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HEY8976437.1"
                     /translation="MAKKVSLTQYLVEQQREHGRIPSQLRLLIEVVARACKHIAIAVN
                     KGALGDVMGSADSENVQGEIQKKLDIISNE"
     gene            complement(402..>2024)
                     /locus_tag="VIN75_19635"
     CDS             complement(402..>2024)
                     /locus_tag="VIN75_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HEY8976438.1"
                     /translation="IESVVGHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFSQDM
                     AGSTSARGVTEYFRRHDGQAVTCDDFAQAIADANPDSELSRQLPAFKRWYAQSGTPRL
                     AAAAVPDPGTGLFTLTLRQDFSAPQAAAGAPRQPFVIPVRFALFTRDGRAVPLQLEGE
                     LLADGATAPTERVLVLSDAERVFRFVNVPDDVVPSVLRDFSAPVILDDGLSDADRLVL
                     LAHDTDPVNRWEAGQRLALSQLVAAITGDGTLSLNEAYVAAMRSVLRDPSLDPAFKEL
                     VLTPPSEAYVSEQLTVPVDPQRVHAVCLQMQDQLAARLHGDWLDAWEGHQVVEGYSPD
                     GRQSARRALANLALAMLVRDAVRSGDTVWPGKAYQRFKDAGNMTDRLGALGALVAVHS
                     DLAAPALARFHELFRSEALVVDKWFMLQARAPEQDGRVFASVKALLQHPDFSMHNPNR
                     VRSLLFSLCNFNPAAFHRADAKGYVFWAEKVVELDGINPQVASRLARALDRWRNLAEP
                     YRGAAREAIVRVAAKSDLSPDVREVVDRALKD"
BASE COUNT          308 a          671 c          752 g          293 t
ORIGIN      
        1 cctcgttgga gatgatgtcc agcttcttct ggatctcgcc ctgcacgttc tcggaatcgg
       61 ccgagcccat gacgtcgccg agcgcgccct tgttgacggc gatcgcgatg tgcttgcagg
      121 cgcgcgcgac gacctcgatc agcaggcgca gctgcgacgg gattcgcccg tgctcgcgct
      181 gctgctcgac gaggtactgg gtgaggctga ccttcttggc catggacaac tcctgaaacg
      241 aggaaatgga tgcgatcaaa gaagccgtca tcctgcgcgg agcgaagcgg agtcgcagga
      301 tccactttgg cgagccggac tgccggccac ggtggatcct gcgactgcgt gcaggatgac
      361 aaatgaaggt cgcagcgtga ccggctggca acggcggatc gtcaatcctt caatgcgcgg
      421 tcgacgacct cgcgcacgtc cgggctcagg tcggacttcg ccgccacgcg cacgatggcc
      481 tcgcgcgccg cgccgcggta cggctcggcc aggttgcgcc agcgatcgag cgcgcgcgcc
      541 aggcgcgaag cgacctgcgg gttgatgccg tcgagttcga ccaccttctc ggcccagaac
      601 acgtagccct tcgcgtcggc gcggtggaac gcggccgggt tgaagttgca cagcgagaac
      661 agcaggctgc gcacgcggtt cgggttgtgc atcgagaagt cggggtgctg cagcagcgcc
      721 ttgacgctcg cgaacacgcg gccgtcctgc tcgggcgcgc gggcctgcag catgaaccac
      781 ttgtcgacca ccagcgcctc gctgcggaac agctcgtgga agcgggccag ggcgggcgcg
      841 gcgaggtccg aatgcaccgc caccagcgcg ccgagcgcgc cgaggcggtc ggtcatgttg
      901 ccggcgtcct tgaagcgctg gtaggccttg ccgggccaca cggtgtcgcc gctgcgcacg
      961 gcgtcgcgca cgagcatcgc gagcgcgagg ttggccagcg cacggcgggc gctctggcgg
     1021 ccgtccgggc tgtagccctc gacgacctgg tggccttccc acgcgtcgag ccagtcgccg
     1081 tgcagccgcg cggcgagctg gtcctgcatc tgcaggcaga cggcgtgaac gcgctgcggg
     1141 tcgacaggca ccgtcagctg ctcggagacg taggcctccg acggcggcgt cagcaccagc
     1201 tccttgaatg ccgggtcgag cgacgggtcg cgcagcaccg aacgcatcgc ggcgacatag
     1261 gcctcgttga gcgacagcgt gccgtcgccg gtgatcgcgg ccaccagctg cgacagcgcc
     1321 aggcgctggc cggcttccca gcggttgacc gggtcggtgt cgtgcgcgag cagcaccagg
     1381 cggtcggcgt cggacaggcc gtcgtccagg atgaccggcg ccgagaaatc gcgcagcacg
     1441 gacggcacga cgtcgtccgg gacgttgacg aagcggaaca cgcgctcggc gtccgacagc
     1501 accagcacgc gctcggtcgg cgcggtggcg ccgtcggcca gcagctcgcc ttcgagctgc
     1561 agcggcaccg cgcggccgtc gcgcgtgaac agggcaaagc gcaccgggat cacgaacggc
     1621 tggcgcggcg cgccggcggc ggcctggggg gcggagaagt cctgcctgag cgtcagcgtg
     1681 aacaggcccg tgccgggatc gggcacggcg gcggcggcca ggcgcggcgt gcccgactgc
     1741 gcataccagc gcttgaacgc gggcagctgg cggctcagct cgctgtcggg gttcgcgtcg
     1801 gcgatggcct gggcgaagtc gtcgcaggtg acggcctggc cgtcgtggcg ccggaagtac
     1861 tcggtgacgc cgcgcgccga cgtggagccg gccatgtcct gcgagaactc ctggtcgcgg
     1921 aagacggtca ggccttcctt gagcgacagc tggaaccagt cgcggcaggt gatgcggttg
     1981 ccggtccagt tgtggaagta ctcgtggccg acgacgctct cgat
//
LOCUS       DATCRO010000730        32170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_582348, whole genome shotgun sequence.
ACCESSION   DATCRO010000730 DATCRO010000000
VERSION     DATCRO010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 32170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582348"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /gene="ureC"
                     /locus_tag="VIN75_19640"
     CDS             <1..1420
                     /gene="ureC"
                     /locus_tag="VIN75_19640"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY8976439.1"
                     /translation="IGKAGNPDVQPGVDIVIGPGTEIIAAEGLIVTAGAIDTHIHFIC
                     PQQIDEALASGVTTMTGGGTGPATGTFATTCTPGPANIERMLQAADAFPMNLGFLGKG
                     NASRPEALRQQVEAGAIGLKLHEDWGTTPAAIDCCLSVAEETDVQVAIHSDTLNESGF
                     VEDTIAAFKGRTIHSFHTEGAGGGHAPDIMKVVGEANVLPSSTNPTRPFTVNTLDEHL
                     DMLMVCHHLDAGIAEDLAFAESRIRRETIAAEDILHDLGAISMFSSDSQAMGRVGEVV
                     LRCWQTAHKMKAQRGALAADGERNDNARVKRYVAKLGINPALTHGIAHEVGSIEVGKW
                     ADLVLWHPAFFGVKPALVLKGGFIAMAAMGDPNASIPTPQPVHMRPMFGSFGGACARG
                     SLTFLSQAAVAAGVPGRAGLAKPVSAVRGCRTVTKRDMVHNDWLPRMEIDAQTYEVRA
                     DGQLLTCEPATELPMAQRYFLF"
     gene            1469..1996
                     /gene="ureE"
                     /locus_tag="VIN75_19645"
                     /pseudo
     CDS             1469..1996
                     /gene="ureE"
                     /locus_tag="VIN75_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
     gene            2003..2701
                     /locus_tag="VIN75_19650"
     CDS             2003..2701
                     /locus_tag="VIN75_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEY8976440.1"
                     /translation="MPAASFPGPKTFLQLIWLASPALPVGGFSYSEALEAAVDEGLVH
                     DESSAAAWLSNQMTLGPARADLPVLAHAHEAWARRDLARITALNEFVALTRETRELRL
                     QAEQMGRSLVEWLRNQDAADDRLAHLAALAPSPTWPVAFGLASARSGATAGQALQTCL
                     FGWAENLVQAALKAVPLGQLAGQRILQALLERMPALVEQALALDEAHWQSFSPMLGIA
                     SARHETQYSRLFRS"
     gene            2721..3392
                     /gene="ureG"
                     /locus_tag="VIN75_19655"
     CDS             2721..3392
                     /gene="ureG"
                     /locus_tag="VIN75_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY8976441.1"
                     /translation="MTSSATPPPSPLHHIAGRSKRLPPLRVGVGGPVGSGKTTLVEML
                     CKAMREKWDLVVVTNDIYTKEDQRLLTVAGALAPERIMGVETGGCPHTAIREDASVNL
                     EAIDRMLEKFPDADIVFVESGGDNLAATFSPELSDLTIYVIVVAAGEKIPRKGGPGIT
                     KSDLFVINKTDLAPHVGADLAVMDADTRRMRPNRPFVMTNLKTRAGLETIIDFIEKRG
                     MLGAG"
     gene            complement(4742..5614)
                     /locus_tag="VIN75_19660"
     CDS             complement(4742..5614)
                     /locus_tag="VIN75_19660"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8976442.1"
                     /translation="MRALTFVLLAALPAAAIAHQLGSGTSRSFGLETLLAFMMVASVA
                     GYAKGVATLWRRAGAGRGIRVRQVVAFGAGWGTLVVALYSPVDTLADRSFAAHMTQHE
                     LLMIVAAPLLAASRTYEAWSWALRKRGVRALATLATAWRWLAVPTRAWWLHAAALWLW
                     HVPALFLAALRDEDIHALQHASFFLSALLFWWAVFGSRRRNHQGTALALLVTTMLQMN
                     ALGLLLTFAPQPWYAADEPPLWGLTALEDQQVGALIMWMFGGMVYAAAALAVVLRWLG
                     PSSDAASQPPRVAP"
     gene            complement(5602..5922)
                     /locus_tag="VIN75_19665"
     CDS             complement(5602..5922)
                     /locus_tag="VIN75_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976443.1"
                     /translation="MRIWPALLLAPALALADQVIAFATLGWACARGQPIVVHAEHVVF
                     FLAAVATLPSAWHAWKDSRTRHAAAGEREAFLAGLAVGSAAMSAIVIAAMSLPTWLIA
                     PCVR"
     gene            complement(5935..6582)
                     /locus_tag="VIN75_19670"
     CDS             complement(5935..6582)
                     /locus_tag="VIN75_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8976444.1"
                     /translation="MSKASRREATPASTPAVERGDALDVSVLPSYGFGTRSVMWWGTV
                     AMCAIEGTAFGFMIIIYFYLRSLSGTWPAGGAAPDLAWGTVNLVIILASAVPNWFADR
                     AAIDQNLPVVRLMLVLGTLCGLALCAVRTMEFTVLNVRWDDSAYGSVIWMLMGLHTFN
                     LVTDVVDTMVLTAVMFKQPLEGKRYVDIAENCGYWVFIVVTWIPIYAVVYWAPRF"
     gene            complement(6579..8492)
                     /locus_tag="VIN75_19675"
     CDS             complement(6579..8492)
                     /locus_tag="VIN75_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8976445.1"
                     /translation="MAPALPALEADARAELARAWDDPPTLKGWICTLDHKAIARRFIV
                     TALLFFLAGGVLGGLMRHQLSAPGKTLVGPDLYNQVFSMHGTTMMFLFAVPVMQGVAL
                     YFVPLMIGTRNTAFPRMSACSYWIYLIGGLTLFAGFAGNTGADAGWFSYVPLAGPEYG
                     FGKRSDFWNVLVNFTEAMGLMVATDLTTTTLKMRAPGMTLLRMPLFAWASLVSSIMII
                     FAMPTVMLGANLVQFDRMAGTHFFNPAEGGDVLLWQHMFWYFGHPEVYFIFIPALGMI
                     SAIIPTFARRPMFGHRAIVLSLISTGFLSFGLWVHHMYATNLPELGKSFFTAMSFMIA
                     IPTAVQLFCWIATIAGGRLVWKSPMLFIAGFFATLMIGGLTGIMLGAVPLDLQVHDTY
                     FVVAHLHYVLIGGALFPLFAAVYYWFPKVYGRMLDERLGRWQFALLFIGFNVTFFPLH
                     VLGLHGMPRRIYTYTADTGWGTLNFVSWVGAMVEDVSMFLFIFNIARALWKGRQAESD
                     PWGGPSLEWATSSPPPSYNFERIPVVASSEPVWSPPAAGPRYVTGLSTRLREGLVTTV
                     MDAVPDVRYLYPSPGAWPIIAAWTVGLWLIWSIFSNKGMLIGLIPPTIAFIAWYWPTE
                     EETSAELEVEKRP"
     gene            complement(8505..9452)
                     /gene="coxB"
                     /locus_tag="VIN75_19680"
     CDS             complement(8505..9452)
                     /gene="coxB"
                     /locus_tag="VIN75_19680"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8976446.1"
                     /translation="MSDALDAAGPQAAWIARLWWISLWLCVAVFLGVMAALGVAMWRS
                     HRAAAAAGTAAIRKDRALRILVGSATLLSSALLVGLLEVSVLTDRALARLPGRDAVTI
                     RVTAHQWWWEMVYEDPRADHVFSTANELVIPVGRPVVVTLEADDVIHSFWVPNLHGKK
                     DLIPGRTTTIEFRADREGRFVGRCAEFCGLQHAFMAYDVVALPPERYAEWVQHERASA
                     AEPLAADAVRGRELFLTGSCMLCHAVQGTTAQGRRGPDLTHFASRSHIGAGSIANTPE
                     ALARWIEDPQKLKPGANMPSHFLPPADLAALVAYLETLT"
     gene            complement(9452..10009)
                     /locus_tag="VIN75_19685"
     CDS             complement(9452..10009)
                     /locus_tag="VIN75_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8976447.1"
                     /translation="MSSGFHKACGVAALVVLLLAVPGCEREARRLDKTPIAPDARARA
                     QATAPFSAGPPGRGLQERAAAGGYDERSAWTLAQGKRYYRWFNCIGCHAQGGGGIGPA
                     LMDAGWTYGKTPGDIFTTIMDGRPNGMPAFRGRIPEEQAWQIAAYVRSLSGLVSSDAA
                     PNRADSLLGGPPEDLRDPTRPEDKR"
     gene            complement(9990..11765)
                     /locus_tag="VIN75_19690"
     CDS             complement(9990..11765)
                     /locus_tag="VIN75_19690"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY8976448.1"
                     /translation="MFPSLRIARIAPRLIAAPLALASASLLAAPPGDWPMPAHDDANT
                     RFSPLAEINASNAAKLQPAWNFSTGTDKGEEGAPIVAGRMMFVVTAFPNRVLAFDLSG
                     PDHGLRWTFDPKADPRARGVACCDVVNRGAAYADGRLFFCTLDNQVLALDARTGREIW
                     RKRLGDLSSGQSMTMAPLVVKDKLLVGEDGSEFGVRGFIAALDVATGSERWRAYSTGP
                     DADVRIGHQFAPYYAGDRGTDLGVTTWPPEAWKIGGGAVGGWISYDAQLDLVFHGTGS
                     PAPRNPLQRPGDNKWTSALFARRPDSGEAVWAYQTSPHDLHGYDGSNESVLVDLPIGG
                     RTRQVLLHPDRNGDVYVMDRATGQVLSADPYALVTTSHGVDPATGTLRYDTGKTPALD
                     HVVREICPGSAGAKNWQPSAWSPRTRLLYLPHQNLCEDTTLFEAGHISGTPYLGAESK
                     MHAAPGRRRGELTAWDPVARRKAWSITEAFPVWSGALATAGDLVFYGTMDGWFKAVDA
                     RDGKELWKFRTSSGIVGQPVSFTGGDGRQYVAVLAGVGGWAGLVVANDLDTRDGTAAL
                     GFVEAMKDLPAATSRGGMLYVFRLP"
     gene            12005..12391
                     /locus_tag="VIN75_19695"
     CDS             12005..12391
                     /locus_tag="VIN75_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976449.1"
                     /translation="MALAVPDIDALGQDQARFIERLATTRPACRRSGCPPGLDPAHTG
                     LPAPREPEPLMSDLESALQDAHRHVSDGYRLVGEQVARLARMPPDSDEMQSAQALLDT
                     MRSTLAIFVADLDRLEARCAEARRKP"
     gene            12623..12916
                     /locus_tag="VIN75_19700"
     CDS             12623..12916
                     /locus_tag="VIN75_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976450.1"
                     /translation="MDDDAYAQRYLQCLQRLYADAAAHCERLENARLLTRAGGWRTQG
                     ADAALAAGYRSLQLLARNCERARQLVNGPTLPDALSTTPDLGERRVRGPRSIG"
     gene            12939..13610
                     /locus_tag="VIN75_19705"
     CDS             12939..13610
                     /locus_tag="VIN75_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8976451.1"
                     /translation="MIDLSQPLPDGPILLDGPVGTLEVFVDRPRAAPAAVAIVLHPHP
                     SQGGSAEHKIPAALAKALADIGFLVLRPNFRGVGRSEGEHASAAGEADDIRQLADAVA
                     TAFASLDLALVGFSFGAFVAAKVAKQLAEAGRPLRRVVLAGLPYGKLRGSQAYDTPSG
                     IPGALVVHGERDDRVPLSSVLDWARLDGQPVVVVPGTDHFFKGRLVVLRSLVADHLAP
                     LAARR"
     gene            13943..16336
                     /locus_tag="VIN75_19710"
     CDS             13943..16336
                     /locus_tag="VIN75_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976452.1"
                     /translation="MEIRIGSADFSAPLRGSGPRTASQAVIFPRAVNQAAVGLIGYSA
                     AFGDNDDHNIGKLQIKVDATVTSNVVNVTATLGLRDWSGDWDDDYQGTVEFAVLADLV
                     SATAPPPRGDMVIVDMEANQAVQFFRAARFLDASNVLPDNAIALVESKATGFRMYVDY
                     DAFSGLPPVSHLTGQMTVRTGSTTLTLDPINSGGAIVPLADSAINQAVADNTLNFMLE
                     GAWCVGTVTVTVQVWDAADASTKSAPFTRTLTFVPVAPLDIFLVGVHYTAQGLDLTAP
                     TQSDIVTNALTQLRATYPVGEVSVNGYSTLDFGENVNFTIGTQGGCGDGMNDLLDKLS
                     DMQGGSSDVYAGFLPDLSLIQTPGSNIGGCGTVSQAAIFLDQVGDVPHEVGHALGRQH
                     APCNSGRCNPPPANVDDHYPQYGAFPAGSIGVFGFDPTTNTVFDPASTADFMAYWGPQ
                     WVSAYTYMALGGAFPAQNGGPGAGLASGHALGNVPVETLMLRLVVTRERQVRRLPSFH
                     FLSLPRSFGNGCSEFTVELLDACRRTLVCAPLDCGCQRAGCHCWPRTMRGAVPYPASA
                     RWLLVYENDRKIHEECIPARPPQVRFGESKPGERGMQLSWEPVALETLATDCCEPCCD
                     ALTYLVHWYDEEDGIWRGIAPRGTARTALVPATLYRRADLRVRVLASCGVATGHAETT
                     IPRGGKPGGGEGGDCPPDRRRGAGTASLYLEGYDAKARGPKPGTQVLRATAIGELGKS
                     VEVTRVRWYGERGEELAVGPQLDLRRLPRDTQTIRVTMRTADGDWLHQSWPLDRARR"
     gene            16358..16666
                     /locus_tag="VIN75_19715"
     CDS             16358..16666
                     /locus_tag="VIN75_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976453.1"
                     /translation="MQIINGSVTFGQHSGSGPQVQTTTVTMGAPVSQAAAFLSGFNVE
                     FSHGDDHNFGLLDVQVQVPSGGISGAAVQVQVSYGLRDWSGDWDDDYDGQIFFTVVGE
                     "
     gene            complement(16677..17759)
                     /locus_tag="VIN75_19720"
     CDS             complement(16677..17759)
                     /locus_tag="VIN75_19720"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976454.1"
                     /translation="MSWPQAMTVAKLRHAVRFASVAAAYAFALLAPDPAHCGSLTVRD
                     AAGDQWRAVSDPADGAQGYVLEHLDADGRLDPNFGHGGRRPLTISATDDAPTALRVDD
                     GGRIWASGASIAGGQPQVVILRYLPDGAPDLRWGVQGKAQVVPGGLAIKPNDLLPLAD
                     GSLLVAGVAANVEPTRAVVFHLAADGSLDLSFGTRGTWQRTDAAQDLTATSLAVGDGG
                     AVAACVVARGDHPAAEIWSLAMPTPKLVEQQALDPGRDGEDMRVAWSGMHWAFGTGNA
                     PTLAGLRATLDPAGLAAERAAASAPSDPGQGGFSPFASDGAAPSSTAGATAEARSPSD
                     TLVAAGVALVAIVAVIVSLRMRRRGT"
     gene            complement(17765..18514)
                     /locus_tag="VIN75_19725"
     CDS             complement(17765..18514)
                     /locus_tag="VIN75_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY8976455.1"
                     /translation="MSIARTLSFHFAAVALGLATCGAFADDAAIRRALSERLPDLPRI
                     DEITKSPVQGLYEVRYNGTEILYASENGDYVFVNGSLVETHSRQNLTEARSQAFMVTD
                     FDKLPLKDAIVIRQGNGARRMAMFVDPYCEYCKSFERDLVNVRDVTIYAFMIPILGPE
                     SKTRARDIWCAGDPARAWRDWMLRRVEPMRAMKCDVAPLERNVAFASQQRISVTPSLF
                     FGDGSRKKGILTADAVESRLREAAPHATAAR"
     gene            18822..19835
                     /locus_tag="VIN75_19730"
     CDS             18822..19835
                     /locus_tag="VIN75_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976456.1"
                     /translation="MKFDSRRLARPIVLTALAAASFCALAQESAKAMPDAAANAAAPR
                     GVHWKNDPDSLAMPGSVAGQRAAAEAAARSSTIAARSSGKTLTYYGGPIVKNIKVIQV
                     LYGSGTYQPFVQGTGSGTMSAFYSGVVGSPYFTWLNGDYGTGSGKTTQSIGYGSFAGQ
                     VTITPSSSRNGSTITDAQIQAELAAQISAGHLAAPNANTYYAVFFPKGKTISQGGSNS
                     CVSGGFCAYHGTVAGSSATWMYGVHPDNSAGSGCDVGCGGSTPFNNMSSVSSHEMIET
                     VTDPDVGIATTYASPLAWYNRTYGEIGDICNAQQGTVVGTDGVTYTVQKEWSNSQSIC
                     RIQ"
     gene            complement(20056..22632)
                     /locus_tag="VIN75_19735"
     CDS             complement(20056..22632)
                     /locus_tag="VIN75_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8976457.1"
                     /translation="MQPASPSDHLLDRNLATLDAVMAFAEFSPDGELLHANRHYLQIF
                     GYELSEVQGAHHRMFCDPAYVASPEYATLWQRLRAGEAHTDLCERRRHGGQACWLEAT
                     YAPVLGARGQVLRIVKMASDVTERVGRERRATEEIRRLSMVAGVTDNAVLITDADRRI
                     TYVNEGFTRMFGWRLAEIAGRRPAGLLAPHLEGEEGAVLDSLRAGTSLRREEILHGRD
                     GARYWCSITSDPVFDEGGRLVNAVSVLTDITQSKMQEVLQHRVLEAIARERPLVEVME
                     MMCAEVEGILPGIATFITEADAQGRLLPLAAPSMPEAYRLRVADKARWPIGSPAQEAA
                     WSADGLVCADIAGDPSFPWPEFRAALLDMGYAACWTMPVNAVDHTRLGTVTFHYTRAH
                     APSEFHRRLMAACIHLCALAFERERSKARIRQLAFYDALTALPNRSLLLAKAEQALAA
                     AARTGAPAAVIFIDLDRFKQVNDSLGHAAGDELLCHVARQITRDKRAVDIAGRLSGDE
                     FVLVLPDCDAEDSTVVVERLQARIADPHEIAGQRLNASASCGIALFPSDGVDIETLLM
                     RADMAMYQAKTDSRGSYCFFSAEMNALAQERLALETALRAALQGNGLSLHYQPQVDLR
                     TGELCGAEALARWSHPELGNVPPSRFIPLAEDCGLIAVLGDWALRTACDQLAAWRAAG
                     LVVPSVSVNLSSTSFHDLGLPQRLVGMLSERGLAPPDLVVEITEGTLIDDHPATRRTI
                     GEIHRLGVRLSVDDFGTGYSSLSYLRRLPIHELKLDRSFVSDLETDVTAQALSRAVVQ
                     IGHSLGLIVVAEGVETEAQMALLAGQGYQVAQGYRISRPMDPRGFGEWLAQRSAAATA
                     CG"
     gene            complement(22865..24757)
                     /locus_tag="VIN75_19740"
     CDS             complement(22865..24757)
                     /locus_tag="VIN75_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8976458.1"
                     /translation="MTSPAVLPPGRSAPRPRSLAARLTIAALQSTALALLVAGLAVNV
                     LVYGWSHATLRGQAELQAAIASDGIAPALLFRDARAAGEILQLLKSSPDIRQATVLDV
                     TGQVFARYDAVAPDAAARKLPAEPGAIEVREPVMQGRERLGELRLVVSRRALQRQTLM
                     LCGATLLSALLGLVVSYVRVRRVRRAVARTEDELNQLAYFDPVTGLQNRHAAMELLAG
                     LAGEPSTRFSVLLLDLDDFKLVNDTMGHAAGDALLGQLGGALQQAFGATGKVFRLGGD
                     EFVAVIRSRRAGESAEHLGRHAIACFNQPMRFRGKLMPVHASVGIAEFPGHGATAQEL
                     LRAADIAMYRAKSSGKNGFAIYDESMDRENVARLQLSEELAAALQRDELVLHYQPILD
                     LGTEAVIGVEALVRWRHPTRGLLMPGAFIEVAEGSGLIVELGGWVLRAAARQQAAWQG
                     RGLGHLFIAVNVSAQQLARRTLIDQVDAALKDSGADPRQLQIELTEHALIEDVAANVQ
                     MLAILKLRGMKIAIDDFGTGLSSLSYLKRLPIDKIKVDRSFVNDLSSTGEDYAIVATI
                     VGLASALKKEIVAEGIETPQQLAVLRRLHVHHGQGYLFSRPVPAEEIEAMSSSVAHEP
                     VRAEPR"
     gene            complement(24754..25287)
                     /locus_tag="VIN75_19745"
     CDS             complement(24754..25287)
                     /locus_tag="VIN75_19745"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY8976459.1"
                     /translation="MNGLHSLRRAWPAWLAGVGLALLPLRGALAVERGALEAAIVFNV
                     LQFVEWPNESALADGSALTLCLRSDSPMMAPVRLLEGRAVRRMTLHVAGLEGAPHPCQ
                     AVYVDSAAAEQDARNAVREPALVIGSSDYRPAARPSIQLVFTDGRLAFDIDQRKALQS
                     GLSISSRLLRLARTVSE"
     gene            complement(25290..27248)
                     /locus_tag="VIN75_19750"
     CDS             complement(25290..27248)
                     /locus_tag="VIN75_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976460.1"
                     /translation="MSKKSLLVPCLSLSLGLASSAALAANPSVPDLASMPLDQLLDMQ
                     VSGVSKFPSTLADTAAAVTVVSAAEIRALGCRTLADVLATVKGAMVTTDRSYSYIGVR
                     GFYAPGDYNTRVLLLIDGVRANDALYDQAYLGSEFPVDMALVDRVEFIPGQASAVYGA
                     NALFGVVNVVTRRPDAGAPAGAEATLGSFGERRVRLEDTLSAAGGAVVRLSASSGGTR
                     GEDVPADGAVTPRGDFEQRTSVTLNARYGGLALSLIQSHRTRGNPAIVDTLAGDWRTR
                     NIDNQGLYDLTWTGSSEAGDQSTLRLYGGSYHFVGRYVMDAAPVTINEDTDTAHWVGA
                     EGHVTTERIAGHRLTAGAEFQLSPMLRSFNADVAPPSEPYLDRNDPSRRLAVFAEDVW
                     RATGTVSVDGSLRLDATRGYRAQPSGRMALVWRPADTLVTKAIVGTAYRPPNDFEAHY
                     QIAGSGGYEVNPRLRPEAVRGAELNVEWHPDASDSLGGSLFENRAERLIVQARDDAND
                     RYTFVNQGTVVARGAEVEWQHAWAQGPRLRANVSTSFAHDRATGTPIAVYAPHWIANL
                     TAMLPLAAGVQAGLQWRAVARRGGAGAYALANAALSSTTAAGGWSWSANVQNLFDRRY
                     DDPGTDLEAQPSIRQAGRSIEITVSKAF"
     gene            27477..27980
                     /locus_tag="VIN75_19755"
     CDS             27477..27980
                     /locus_tag="VIN75_19755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976461.1"
                     /translation="MPAQDDKLRVLVVDDNVDAAATFCALLRLSGCEAQAASGLAIAL
                     AVAASFEPDVVVLDLEMPGADGCRVLQQMRERGDGLADPLYVCLTGASSDENRRRCEA
                     AGFHRFHAKPLRPHELAEILADARARRLRQRVAMAMPEAAPLPAPGAAISQGVRAGPA
                     GNDPLSH"
     gene            28249..28521
                     /locus_tag="VIN75_19760"
     CDS             28249..28521
                     /locus_tag="VIN75_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976462.1"
                     /translation="MRDLGDVSLPPDDFSAMATLCKHDQDFPPTLEAWRAMISEATLE
                     AHRRGQYPAPMQIDVRKFSDWCSLVQVLPCLDSLRAFVIIRRREIS"
     gene            28585..28935
                     /locus_tag="VIN75_19765"
     CDS             28585..28935
                     /locus_tag="VIN75_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976463.1"
                     /translation="MRRFVVASVPSVAWLEAMLLFRRQAVPRTIAEAALELYIPEPAA
                     ADVLRALVEAGVLSQAGETFDYAPANPRLAAMLDRVAAAHAADLVGITNLIHDKTHRN
                     ARRFADAFKLRKDA"
     gene            28936..29196
                     /locus_tag="VIN75_19770"
     CDS             28936..29196
                     /locus_tag="VIN75_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY8976464.1"
                     /translation="MASAIYTLCAVTAFACAFLLLRSYARTRFRLLLWSGLCFAGLCA
                     NNVLLILDRYVFLAVDLSVARQAVAFVSVALLVVGLVLESDA"
     gene            29193..29486
                     /locus_tag="VIN75_19775"
     CDS             29193..29486
                     /locus_tag="VIN75_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY8976465.1"
                     /translation="MNPQDIPTMFAVNELLLGGIAVGCAIAGLFFLRYWRSSKDRFFL
                     CFAISFWAEAANRLHMGLTAAWNETQPSHYLVRLLCYGLILFAIWDKNRPGRD"
     gene            complement(29493..30038)
                     /locus_tag="VIN75_19780"
     CDS             complement(29493..30038)
                     /locus_tag="VIN75_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976466.1"
                     /translation="MDCSALLTRQLAELTALISRLRSSRRGEDALRLFGDLANRAVLH
                     VLSRERVLLPAWRHIRWKDLPLDAMTGHVRFKQALAELLVRPPSSDGHAQALAAFAEH
                     VEQQHLLDQHRLVPVLRDAMDIEQRRALCNDVELLFETGSPPMRELTAGVASPKDLVA
                     EAEIVLSSLAAIQPVARPAPA"
     gene            30395..31333
                     /locus_tag="VIN75_19785"
     CDS             30395..31333
                     /locus_tag="VIN75_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8976467.1"
                     /translation="MTSSPSAAAPGSHRSSWAIHLKLVGMAALWGGSWPCGRVLAQAL
                     PPLAAATWRFAIALVLLLAWMQVDGGFGRLRKLTRRQWGGLAVGGAVGVFGYAMFFML
                     GLSHVAAGRAALVVTTNPVVTTLIAAWWFGERLNWRIGAGMALAAAGALIVMTHGAPW
                     TLLAGSVGKGEALLLGCVATWVAYTLIGRRLLAGIDALTTTTVTAAFGLVMLAVTALV
                     FEGPAAVATPLTASATTWVALGFLAAGATVVAYAWYFEGVAALGAGAAAGYISLVPLF
                     GVLFATSWLGEPLDASIVVGGALAVIGMAVMNLARR"
     gene            complement(31311..31577)
                     /locus_tag="VIN75_19790"
     CDS             complement(31311..31577)
                     /locus_tag="VIN75_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976468.1"
                     /translation="MASALTPPQVEVLRRLRDGVGLSTEPLRPGLLREVAFLMAMGLL
                     ELHGPLDVQLSGLGRAYLAAVEAEAGRDGREGSASDPISGAPGS"
     gene            complement(31652..>32170)
                     /locus_tag="VIN75_19795"
     CDS             complement(31652..>32170)
                     /locus_tag="VIN75_19795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HEY8976469.1"
                     /translation="GYISLVPLFGVLFATSWLGEPLDASIVVGGALAVPPPPVVRPKP
                     PLPAGSNWPRALRHCQVSPWRTMGPGMRWSRVTVPQDRDANVLLLRIGAGKCLPLHTH
                     GGTELTQVLHGSFDDGRAVFSAGDFDEADADVLHQPVVQAQGECICLAAVDGRLVFDS
                     WLARWASAMVGL"
BASE COUNT         4899 a        11551 c        11179 g         4541 t
ORIGIN      
        1 catcggcaag gccggcaacc cggacgtcca gcccggcgtg gacatcgtga tcggcccagg
       61 caccgagatc atcgcagccg agggcctgat cgtgacggca ggagcgatcg acacccacat
      121 ccacttcatc tgcccgcagc agatcgacga ggcgctggcc agcggcgtca cgacgatgac
      181 cggcggcggc accggccccg cgaccggcac cttcgcgacc acctgcacgc ccggcccggc
      241 caacatcgag cgcatgctgc aggcggcgga cgccttcccg atgaacctgg gcttcctggg
      301 caagggcaac gcgagccgcc cggaggcgct gcgccagcag gtcgaggccg gcgcgatcgg
      361 cctgaagctg catgaagact ggggcacgac gcccgcggcg atcgactgct gcctgtccgt
      421 ggccgaggag accgacgtcc aggtcgcgat ccacagcgac acgctcaacg agagcggctt
      481 cgtcgaggac acgatcgcgg cgttcaaggg ccgcacgatc cacagcttcc acaccgaggg
      541 cgccggcggc gggcacgccc ccgacatcat gaaggtggtc ggcgaagcca acgtgctgcc
      601 gtcgtcgacc aacccgacgc ggccgttcac cgtcaacacg ctggacgagc acctggacat
      661 gctcatggtg tgccaccacc tggacgccgg catcgccgag gacctggcct tcgcggagag
      721 ccgcatccgc cgcgagacca tcgcggccga ggacatcctg catgacctcg gcgccatcag
      781 catgttctcg tccgactcgc aggccatggg ccgcgtcggc gaagtcgtgc tgcgttgctg
      841 gcagacggcg cacaagatga aggcccagcg cggcgcgctg gcggccgacg gcgagcgcaa
      901 cgacaacgcc cgcgtcaagc gctacgtcgc caagctgggt atcaacccgg cgctcacgca
      961 cggcatcgcg cacgaggtgg gttcgatcga ggtgggcaag tgggccgacc tggtgctgtg
     1021 gcatcccgcc ttcttcggcg tcaagccggc gctggtgctc aagggcggct tcatcgccat
     1081 ggcggcgatg ggtgacccga acgcgtccat ccccacgccg cagccggtgc acatgcggcc
     1141 gatgttcggc agcttcgggg gcgcgtgcgc gcgcggctcg ctcaccttcc tgtcgcaggc
     1201 cgcggtcgcc gcgggcgtgc cgggccgggc cgggctggcc aagccggtgt cggcggtgcg
     1261 cgggtgccgc acggtcacca agcgcgacat ggtgcacaac gattggctgc cgcgcatgga
     1321 gatcgacgcg cagacgtatg aagtgcgcgc cgacggccag ctgctcacct gcgagccggc
     1381 caccgagctg ccgatggcgc agcgctattt ccttttctga agcgcggctc gaatcgctgt
     1441 cgcgcaccgg ccgcaggagt ccgacccaat gacagaacac acgacgaccg ccttgcaggt
     1501 cggcaagcga atcgcgggcg gccaggggtt gagcagcgcg ctggtgaggc gcgccagcac
     1561 gatcgagctg gactgggatg tccggcagaa gagccgcatc gaggcaaccg actcgctggg
     1621 acgcctgctg caggtattcc tgccgcgcgg caccgtactg cgtggcggtg acgtcctcgt
     1681 ggcggaagac ggctcgctga tccgcgtgac cgcggcggcg cagccgctgc tggccgtcac
     1741 ccactgcccg cagcacggca cgcctttcga cctgctgcga gcggcctacc acctcggaaa
     1801 tcgccacgta ccgctggaac tgcggccgga ccggttgctg ctcgaacccg accacgtgct
     1861 ggccgacatg ctgcgtcgcc agcacctggt cgtcgccgag acccagggcg ccttcgaacc
     1921 cgaagccggg gcctacgggg caggcgatgg acacggccac ggccattcgc atggccacgc
     1981 ccacgggcac gagcactgac gcatgccggc cgccagcttc cccggcccga agacgttcct
     2041 gcagctgatc tggctcgcat cgccggcgct gccggtcggc ggcttcagct attcggaagc
     2101 gctggaggcg gccgtcgacg aggggctggt gcacgacgag tccagcgccg cggcctggct
     2161 ctcgaaccag atgacgctgg gccccgcacg cgccgacctg cccgtgctcg cgcacgcgca
     2221 cgaggcctgg gcccgccggg acctggcccg catcacggcg ctcaacgagt tcgtcgccct
     2281 cacccgcgag acgcgcgaac tgcggctcca ggccgagcag atgggccgct cgctggtgga
     2341 gtggctgcgc aaccaggacg ccgccgacga ccggctcgcg cacctcgcgg ccctcgcgcc
     2401 gtcaccgacc tggccggttg cgttcggcct cgcgtcggcg cgcagcggtg ccaccgcagg
     2461 ccaggccctg cagacctgcc tgttcggatg ggccgagaac ctcgtgcagg cggcgctgaa
     2521 agccgtgccg ctgggtcagc tcgccgggca gcgcatcctg caggcactgc tcgagcgcat
     2581 gcccgcgctg gtggagcagg cgctcgccct ggatgaagcg cactggcagt ccttctcgcc
     2641 gatgctgggc atcgcgagcg cgcggcacga aacgcagtac tcgcgccttt tccgttcatg
     2701 acctccggac ccacccctcc atgaccagct ccgccacgcc ccctcccagt ccgctgcacc
     2761 acatcgccgg ccgaagcaag cgcctgccgc cgctgcgcgt cggcgtcggc ggcccggtcg
     2821 gctcgggcaa gacgacgctc gtcgagatgc tgtgcaaggc gatgcgcgag aagtgggatc
     2881 tggtcgtcgt caccaacgac atctacacca aggaagacca gcgcctgctg acggtcgccg
     2941 gcgcgctggc gcccgagcgc atcatgggcg tggagaccgg cggctgcccg cacaccgcga
     3001 tccgcgagga tgcgtcggtc aacctcgagg ccatcgaccg catgctggag aaattcccgg
     3061 acgccgacat cgtcttcgtc gagtccggcg gcgacaacct cgcggccacc ttcagccccg
     3121 aactgagcga cctgacgatc tacgtgatcg tcgtcgccgc cggcgagaag atcccgcgca
     3181 agggcgggcc gggcatcacc aagagcgacc tgttcgtcat caacaagacc gacctcgcac
     3241 cgcacgtcgg cgcggacctg gccgtgatgg atgccgacac tcgccgcatg cgtccgaatc
     3301 gcccgttcgt gatgaccaac ctgaagacca gggcgggcct cgagacgatc atcgatttca
     3361 tcgagaagcg gggcatgctc ggcgcaggct gaccccggag cggcgccgcc agggccgtcg
     3421 aaagtgcgct tgctcagctg agagatctcg cccaggcccc attgcgtcgc atccgcccgc
     3481 cgttggtggc ggtcataact tggtcatgcg tccgtcggcc ggaatgccga caatcgacgc
     3541 cttgcccgtc cacgatcgac ggacgtcaca aggggattca aagtcgctgc caagcccaat
     3601 gacaaaggct ccggacgcat caggccattc gccgcgacgg acttcctgga cacgttgccc
     3661 ggttgcgctc cggaacctct cgacgaagcg ccgcaacctg cctccaccat gctcgttctg
     3721 cccatcacgg gcgctgagcg aggccgttcg gagccgcctc ccgcatttgg acaggcgccc
     3781 aaacccgtga cgagcaagat gagcgttcag ccggcgcccg ctcctgcacc ccaggctcga
     3841 ggcggcgtcg aggccagccc cggattcgcg gccaggggga ctttccaccc cgaggtcgcg
     3901 ccgacgccgc cgaggggaac cggtggcccc gttgacgctt atcgtgcgcc ggcggcccac
     3961 ctgcccaggc cgatggcacc ggcaaggagg aggggcaggc gactcgggct ggtcctgctt
     4021 tgttgttgcg ctgccctggc ctcggccgtc gcgatccagc cgtggctcgg cagggcaatg
     4081 cacgacgccc gcatcgcggc gaattcaacg gcccctgcag tccctgcggc gatcccgcag
     4141 cccatgaccc gggcgttcga cgagcgtgaa gcgccgagcc gggagcccgt cgccattccg
     4201 gcgtctttgt cggtcgagcc tggagcgagt gctccggagg ccgaggagcc gacggccatg
     4261 cccgccgcac cgaccgaacc gtcgacgctg gcgcctgccc cagcggacag gaagcgggac
     4321 cgacacgtcc gaagcccgcg tgccacgtca cggcctgcgg acgttgccgc tgccatccag
     4381 gccgcccagg cccgggcgga tgcatttctc gcagggcgcc cggcgtcttc cgtcgagccg
     4441 aactcgacgc ggtgaatccc gcgaggcaac gaccgaccgg ttcggcgcct cgtagggcgg
     4501 gttagccgca ggcgtaaccc gccatggtgg cgcctcgcaa tgccggcgaa cggcgggtta
     4561 cgcctgcggc taacccgccc tacggcgtcg gctttgcggc aagcaatcgc ggcatcgcga
     4621 tttccgctca gggtcgacgg gcgcccccag cgccgcccga cggcggcgcc gaatggtctg
     4681 accagcaccg gcgcatctgg actctgcatg cgggcgcccg cgcaccacga tggcgatgcc
     4741 gtcacggcgc gacgcgcggc ggctgcgagg cggcatcgga cgacggcccc agccagcgca
     4801 agacgaccgc caacgcggct gcggcgtaca ccatcccgcc gaacatccac atgatgagcg
     4861 cgccgacctg ctggtcctcc agggccgtga ggccccagag cggtggctcg tcagcggcgt
     4921 accacggctg cggcgcgaag gtcagcagca ggcccagtgc gttcatctgc agcatggtgg
     4981 tcaccagcag cgccagcgcg gtgccctggt gattgcggcg ccggcttccg aagacggccc
     5041 accagaacag cagcgccgac aggaagaacg aagcgtgctg cagggcatgg atgtcctcgt
     5101 cgcggagcgc ggcgaggaac aaggcgggca catgccacag ccagagcgcc gcggcgtgca
     5161 gccaccaggc gcgcgtgggc acggcgagcc agcgccaggc cgtcgccagg gtggccagcg
     5221 cgcggacgcc gcgcttgcgc agcgcccacg accaggcctc gtacgtgcgc gaggccgcga
     5281 gaaggggcgc cgccacgatc atcagcagct cgtgctgcgt catgtgcgcg gcgaacgagc
     5341 ggtcggccag cgtgtcgacg ggcgaataca gcgccacgac cagcgtgccc catcccgccc
     5401 cgaaggccac gacctgccgc acgcggatgc cgcggccggc gccggcgcgc cgccagaggg
     5461 tcgccacgcc cttggcgtat ccggccacgc tcgcgaccat catgaacgcg agcagcgtct
     5521 cgagcccgaa cgagcgcgac gttccgctgc cgagctggtg tgcgatcgct gctgccggca
     5581 gtgcggcgag cagcacgaac gtcaacgcac gcatggcgcg atcaaccagg tcggcagcga
     5641 catcgccgcg atgacgattg ccgacatcgc cgccgacccc accgccaggc cggccaggaa
     5701 cgcctcgcgt tcgcccgccg ccgcgtgccg ggtgcggctg tccttccacg catgccaggc
     5761 cgacggcagc gtcgcgaccg ctgcgaggaa gaacacgaca tgctcggcat gcacgacgat
     5821 gggttggccg cgggcgcaag cccagcccag ggtggcgaac gcgatcacct ggtccgccag
     5881 cgccagcgcg ggcgcgagaa gcagtgcagg ccagatgcgc atcaccctgc tccctcagaa
     5941 ccgcggagcc cagtacacga cggcgtagat cgggatccac gtcacgacga tgaagaccca
     6001 gtagccgcag ttctcggcga tgtcgacgta gcgcttgcct tcgagcggct gcttgaacat
     6061 caccgccgtc agcaccatcg tgtcgacgac gtccgtcacg aggttgaacg tgtgcaaccc
     6121 catcaacatc cagatcaccg acccgtacgc gctgtcgtcc cagcgcacgt tcagcacggt
     6181 gaattccatg gtgcggacgg cgcagagcgc aaggccgcac agcgtcccca ggacgagcat
     6241 caggcgaacg acaggcaggt tctggtcgat cgccgcgcga tccgcgaacc agttcgggac
     6301 ggcactggcg aggatgatca cgaggttcac cgtgccccag gccaggtccg gcgccgcgcc
     6361 accggccggc caggtgcccg acaggctgcg caagtagaag tagatgatga tcatgaagcc
     6421 gaacgcggtg ccctcgatgg cgcacatggc gacggtgccc caccacatca cgctgcgcgt
     6481 cccgaaaccg tagctcggca gcaccgagac gtcgagcgcg tcgccgcgtt cgacggcggg
     6541 cgtgctcgcc ggcgtcgctt cgcgccgcga ggccttgctc atggccgctt ctccacctcg
     6601 agctcggcgc tggtctcctc ctcggtcggc cagtaccagg cgatgaacgc gatcgtcggc
     6661 gggatcagtc cgatcagcat ccccttgttg gaaaagatgg accagatgag ccacagtccg
     6721 accgtccagg cggcgatgat cggccaggcg cccggactcg gatagaggta gcggacgtcc
     6781 ggcaccgcgt ccatcaccgt ggtgacgagt ccttcgcgca gccgggtcga caggccggtc
     6841 acgtagcgcg ggccagcggc cggcggcgac cagacgggct cgctcgatgc cacgacgggg
     6901 atgcgctcga agttgtacga cggcggcggc gaggaggtcg cccactccag cgacgggccg
     6961 ccccaggggt cgctctcggc ctggcgccct ttccacagcg cgcgggcgat gttgaagatg
     7021 aagaggaaca tgctgacgtc ctcgaccatc gcgcccaccc aggacacgaa gttgagcgtg
     7081 ccccagcccg tgtcggcggt gtacgtgtag atgcgccgtg gcatgccgtg cagcccgagc
     7141 acgtgcagtg gaaagaaggt cacgttgaac ccgataaaca gcagcgcgaa ctgccatcgt
     7201 cccagccgct cgtcgagcat gcgtccatag accttgggga accagtagta caccgcggcg
     7261 aacagcggga acagcgcccc gccgatcagc acgtagtgca ggtgcgcgac gacgaaatag
     7321 gtgtcatgca cctgaaggtc cagcggcacc gcgcccagca tgattccggt gagcccaccg
     7381 atcatgagcg tcgcgaagaa gcccgcgatg aacagcatcg gcgacttcca gaccaggcgc
     7441 ccgccggcga tcgtcgcgat ccagcagaac agctgcaccg ccgtcgggat cgcgatcatg
     7501 aagctcatcg ccgtgaagaa gctcttgccc agctcgggca ggttcgtcgc gtacatgtgg
     7561 tgcacccaga gcccgaacga caggaaaccc gtcgagatca gcgacagcac gatcgcccga
     7621 tggccgaaca tcggccggcg ggcgaaggtc gggatgatgg cggagatcat cccgagggcg
     7681 ggaatgaaga tgaagtagac ctcgggatgc ccgaagtacc agaacatgtg ctgccacagg
     7741 agcacgtcgc cgccttccgc gggattgaag aagtgcgtgc ccgccatgcg gtcgaactgc
     7801 accaggttcg cgcccagcat caccgtgggc atcgcgaaga tgatcatgat cgagctcacc
     7861 agcgacgccc acgcgaacag gggcatgcgc agcagggtca tgcccggcgc gcgcatcttc
     7921 agcgtggtcg tcgtgagatc ggtcgcgacc atcagcccca tcgcctcggt gaagttgacc
     7981 aggacgttcc agaagtccga gcgcttgccg aagccgtatt cgggtccggc cagcgggacg
     8041 tacgagaacc agccggcgtc cgcacccgtg ttgcccgcga agcccgcgaa cagcgtcagg
     8101 ccgccgatga gatagatcca gtacgagcag gcgctcatgc gcgggaaggc ggtgttgcgc
     8161 gtcccgatca tgagcggcac gaagtacagc gcgacgccct gcatgaccgg cacggcgaac
     8221 aggaacatca tcgtcgtgcc gtgcatcgag aagacctggt tgtacaggtc gggaccgacg
     8281 agggtcttgc cgggcgccga gagctggtgc cgcatcaggc cgcccaggac gccgccggcc
     8341 aggaagaaca gcagcgcggt gacgatgaag cgccgcgcga tcgccttgtg gtcgagggtg
     8401 cagatccatc ccttgagcgt cggcgggtcg tcccacgcgc gcgcgagctc ggcccgggcg
     8461 tccgcttcga gtgcagggag tgccggtgcc acgcgtgcct cgcttcaggt cagggtctcg
     8521 agataggcca ccagcgccgc gagatcggcc gggggcagga aatgcgacgg catgttggcg
     8581 ccgggcttga gcttctgcgg atcctcgatc cagcgcgcga gcgcctccgg ggtgttcgcg
     8641 atgcttccgg cgccgatgtg cgagcggctg gcgaaatgcg tcaggtccgg cccccgccgg
     8701 ccctgggcgg tcgtgccttg caccgcgtgg cacaacatgc agctgccggt gaggaacagc
     8761 tcgcgaccgc gcacggcgtc ggcagccaga ggctcggccg ccgacgcccg ttcgtgctgc
     8821 acccactcgg catagcgctc gggtggaagc gccacgacgt cgtaggccat gaacgcatgc
     8881 tgcagtccgc agaactctgc acatcgcccg acgaagcggc cttcacgatc ggcgcggaac
     8941 tcgatcgtcg tggtccggcc cgggatcagg tccttcttcc catgcaggtt cggtacccag
     9001 aagctgtgga tgacgtcgtc cgcctcgagc gtgacgacga ccggccggcc caccgggatg
     9061 accagctcgt tcgcggtgga gaacacgtgg tccgcccgcg ggtcttcgta gaccatctcc
     9121 caccaccact ggtgcgccgt gacgcggatc gtcaccgcat cgcgtcccgg cagccgcgcc
     9181 agcgcccggt cggtgaggac gctgacctcg aggagcccga cgagcagcgc cgacgacaac
     9241 agcgtcgcgc tgccgaccag gatgcgcagg gcgcgatcct tgcggatggc tgcagttccc
     9301 gctgccgccg cggcgcgatg cgatcgccac atcgccaccc cgagggcggc catcacgccg
     9361 aggaagacgg ccacgcacag ccacaggctg atccaccaca gacgggcgat ccaggcggcc
     9421 tgcgggcccg cggcgtcgag ggcgtcgctc atcagcgctt gtcctcgggc cgcgtcgggt
     9481 cgcgcaggtc ttccggcgga ccgcccagca ggctgtcggc gcggttgggc gcggcgtcgc
     9541 tggacaccag gcccgacagc gagcgcacgt acgcggcgat ctgccaggcc tgttcctcgg
     9601 gaatgcggcc gcggaaggcc ggcatgccgt tcgggcgccc gtccatgatc gtggtgaaga
     9661 tgtcgcccgg cgtcttgccg taggtccagc cggcgtccat gagcgcgggt ccgatgccgc
     9721 cgccgccttg cgcgtggcac ccgatgcagt tgaaccagcg gtagtagcgc ttgccctgcg
     9781 cgagcgtcca ggcgctgcgt tcgtcatagc cgcccgccgc ggcgcgttcc tgcaggccgc
     9841 gacccggcgg ccctgcgctg aacggagcgg tggcctgcgc gcgagcgcgc gcatcgggcg
     9901 cgatcggcgt cttgtccagg cgccgcgctt cgcgctcgca cccgggaacg gccagcagca
     9961 gcaccaccag cgccgccacg ccgcaggcct tatggaagcc ggaagacata cagcatgcct
    10021 ccgcgtgacg tggccgctgg caggtccttc atcgcctcga cgaagcccag ggcggccgtg
    10081 ccgtcgcgcg tgtccaggtc gttggccacg accagccccg cccatccgcc gacgccggca
    10141 agcacggcca cgtactggcg tccgtcgccg cccgtgaagg agactggctg gcccacgatc
    10201 ccggaactcg tcctgaactt ccacagttcc ttgccgtcgc gggcatcgac cgccttgaac
    10261 cagccatcca tcgtgccgta gaacacgagg tcgccggccg tcgcgagcgc gccgctccag
    10321 accgggaagg cctcggtgat cgaccaggcc ttgcggcgtg cgaccgggtc ccaggcggtc
    10381 agctcgcccc gccgcctgcc cggcgccgca tgcatcttgg actcggcgcc gagatagggc
    10441 gtgccgctga tgtggcccgc ctcgaacagc gtcgtgtcct cgcacaggtt ctggtgcggc
    10501 aggtacagaa ggcgcgtgcg cggcgaccag gcgctgggct gccagttctt ggcgccggcg
    10561 gatcccgggc agatctcgcg gacgacgtgg tcgagagccg gcgtcttgcc cgtgtcgtag
    10621 cgcagcgtcc ccgtagccgg gtcgacgcca tgcgaggtcg tgacgagcgc atagggatcc
    10681 gcggacagca cttggccggt cgcgcgatcc atcacgtaga cgtcgccgtt gcggtccggg
    10741 tgcagcagca cctggcgcgt gcggccgccg atcggcaggt cgaccagcac gctctcgttg
    10801 ctgccgtcgt agccgtgcag gtcatgcggg ctcgtctggt acgcccagac ggcctcgccc
    10861 gagtcgggac ggcgcgcgaa gagcgcactc gtccacttgt tgtccccggg acgctgcagc
    10921 ggattgcgcg gtgccgggct gccggtgcca tggaagacga gatccagctg cgcgtcgtat
    10981 gagatccatc ctccgacggc gcctccgccg atcttccagg cttccggcgg ccaggtggtg
    11041 acgcccagat cggtgccgcg atcgccggcg tagtacggcg cgaactggtg cccgatccgc
    11101 acatcggcat ccgggccggt gctgtaggcg cgccagcgct ccgagccggt cgcgacgtcg
    11161 agggcggcga tgaagccgcg cacgccgaac tccgagccgt cctcgccgac caggagcttg
    11221 tccttgacga cgagcggggc catcgtcatc gactggccgc tcgagaggtc accgagtcgc
    11281 ttgcgccaga tctcgcgtcc cgtgcgagcg tccagggcca gcacctggtt gtcgagcgtg
    11341 cagaaaaaga ggcgcccgtc ggcataggca gccccgcggt tgacgacgtc gcagcacgcg
    11401 acgccccgtg cgcgcggatc cgccttggga tcgaaggtcc agcgcaggcc gtgatcgggt
    11461 ccggacagat cgaacgcgag gacgcgattc gggaatgccg tgacgacgaa catcatccgg
    11521 cctgcgacga tcggcgcgcc ttcctcgccc ttgtcggtgc cggtcgagaa gttccaggcg
    11581 ggctgcagct tcgccgcgtt gctcgcgttg atctccgcga gcgggctgaa gcgtgtgttg
    11641 gcgtcgtcgt gcgccggcat gggccagtcg ccgggcggcg cggcaagcag cgatgccgac
    11701 gcgagcgcga gcggcgccgc gatcaggcgt ggggcgatgc gcgcgatccg gagggaagga
    11761 aacacctgtc acccgacaat ggctgagagg agtcgcccag caaagctcgt gccccgccgc
    11821 atgcacggtt tccctgatcc gagcgtggcg tcgcctcgcc atccaactcg agcacgcagc
    11881 ctgcgcaccg gtctcaggat gatggccgtc ccgtcgcctt cgaccttcgg ccagaccgcg
    11941 ccgtggattc ggtccggccc gctggcggtg acgtgcgcgg tcttgacgca acgcgaccat
    12001 tcgcgtggcg ctggccgtcc cggacatcga cgcccttggc caggaccagg cgcgcttcat
    12061 cgaacggctc gcgaccacaa ggcccgcctg tcgccgcagt ggctgccctc cagggctcga
    12121 tccggcgcat actggattgc ccgccccccg cgagccggag ccgctgatgt ctgatctcga
    12181 atcggccctg caagacgctc accgccacgt gtccgatggc tacaggctcg tgggcgaaca
    12241 ggtcgcgcgc ctggcccgca tgcccccgga cagcgatgag atgcagagcg cgcaggcctt
    12301 gctggacact atgaggtcga ccttggcgat attcgtcgcc gacctggacc gattggaagc
    12361 gaggtgcgca gaggcacgtc gcaaaccctg acaggggcct gcgcgtgcgg gtcgcaggcg
    12421 gctgacccgg tccagtccgg cgtgaccgcg cgccgccgtt caaccagacg gcgttccgcc
    12481 cgcgcacggt tcttcgtcag gagccgcaga ctcatgcagc cggcccgtgc ggctggcgta
    12541 ggatccggcc atcggacaag gcggccccgc agcttcgcgc acggcaggcc gattgcccag
    12601 cggacggcat ttcgcactct ccatggacga cgacgcttac gcccagcggt acctccagtg
    12661 cctgcagcgc ctttacgccg atgcggccgc ccactgcgag cggctcgaga acgcccggtt
    12721 gctgacacgc gccggaggct ggcgcacgca gggcgcagat gcggcgttgg cggcggggta
    12781 tcggagcctg caactgctcg ccaggaactg cgagcgcgcg cgacagctcg tgaacggccc
    12841 gacgttgccg gatgccctct cgacgacacc ggaccttggc gagcgccggg ttcgggggcc
    12901 gcggtcgatc ggatgaccgg cccatcgaag gcgccgcggt gatcgacctg agccagcccc
    12961 tgcccgacgg accgatcctg ctcgacggac cggtcggaac actcgaggtc ttcgtcgatc
    13021 ggcccagggc cgcgcccgcg gcggtcgcga tcgtgctgca ccctcacccg tcgcaaggcg
    13081 gcagcgccga gcacaagatt cccgccgccc tggccaaggc cctggccgac atcggattcc
    13141 tcgtccttcg acccaacttc aggggggtgg gtcgatccga aggcgagcat gcgagtgccg
    13201 cgggcgaggc agacgacatc cggcaactgg ccgacgccgt cgccacggcg ttcgcctcgc
    13261 tggatctggc cttggtcggc ttctcgttcg gcgccttcgt cgccgcgaag gtcgccaagc
    13321 agctggccga ggccggacgt ccgctgcgtc gcgtggtgct cgctggactc ccctacggca
    13381 aactgcgtgg cagccaggcc tacgataccc cttccggcat cccgggcgcg ctcgtcgtcc
    13441 acggcgagcg cgacgaccgc gtgccgctga gctcggtcct ggactgggct cgcctggacg
    13501 gccagccggt ggtggtggtc ccgggcaccg accacttctt caagggaaga ctggtggtcc
    13561 tgcgatcgct tgtcgcggat cacctcgcac cgctagcggc gcgacgatag gtcgagggtc
    13621 gcgagggcgc gcggcgccgc tccggcagct tgggcagacc tcagtgagtg ccggagcgcc
    13681 cggtcttggt cgcccgccgg atcgaatgac cgatgcggta ctcgtgtccg cttggccatg
    13741 caattgcgcg tcgatctcga ggcgctggcg gcggcttgcc tccagttcca cggctgcggc
    13801 gccgacccgt tgcattcgac caggcgcgcg gtcgcgggct ccctagtgcg gcaatagggg
    13861 cttggccgcg cgagcgcgct gcctagactc ggtcgcgacg cctgcaggca tcgcgggcat
    13921 cgcaacggga aaggaactcg ccatggagat ccgcatcggc agcgccgact tcagtgcccc
    13981 cttgcgcgga agcggcccgc gcaccgcgtc gcaggccgtc atcttcccgc gcgcggtgaa
    14041 ccaggcggcg gtcggcctca tcggctattc ggccgccttc ggcgacaacg acgaccacaa
    14101 catcggcaag ctgcagatca aggtcgacgc caccgtcacg tccaacgtcg tcaacgtcac
    14161 cgccacgctg ggccttcgcg actggtccgg cgactgggac gacgactacc agggcaccgt
    14221 ggagttcgcg gtgctggccg acctcgtctc ggccaccgcg ccgccgccgc gcggcgacat
    14281 ggtcatcgtc gacatggagg ccaaccaggc cgtgcagttc ttccgcgcgg cgcgcttcct
    14341 cgacgcctcc aacgtcctgc cggacaacgc catcgcgctc gtcgagagca aggcgacggg
    14401 cttccggatg tacgtcgact acgacgcctt ctccggcctg ccgccggtga gccacctcac
    14461 gggccagatg acggtgcgca ccggcagcac gacgctcacg ctggatccga tcaactcggg
    14521 cggcgccatc gtgccgctgg ccgactccgc gatcaaccag gcggtggccg acaacacgct
    14581 caacttcatg ctcgaaggcg cgtggtgcgt ggggacggtc accgtcacgg tgcaggtctg
    14641 ggacgccgcc gacgcctcga cgaagtcggc cccgttcacg cgcacgctca cgttcgtgcc
    14701 ggtggcgccg ctggacatct tcctggtggg cgtgcactac accgcgcaag ggctggacct
    14761 caccgcgccg acgcagtccg acatcgtcac caatgcgctc acgcagctgc gcgccacgta
    14821 tccggtgggc gaggtcagcg tcaacggcta ttcgacgctg gacttcggcg agaacgtcaa
    14881 cttcacaatc ggcacgcagg gcggctgcgg cgacggcatg aacgacctgc tggacaagct
    14941 ctcggacatg cagggcggat cgagcgacgt ctatgccggc ttcctgcccg acctgtcgct
    15001 gatccagacg ccgggcagca acatcggcgg ctgcggcacg gtgagccagg ccgcgatctt
    15061 cctggaccag gtgggcgacg tgccgcacga ggtcggccac gcgctcgggc gccagcacgc
    15121 gccctgcaat tccggccgct gcaacccgcc gccggccaac gtcgacgacc actacccgca
    15181 gtacggcgcg ttccccgccg gcagcatcgg cgtgttcggc ttcgacccga ccaccaacac
    15241 cgtgttcgac cccgcgtcga cggccgactt catggcctac tggggcccgc aatgggtcag
    15301 cgcctacacc tacatggccc tgggcggcgc cttcccggcg cagaacggcg ggccgggcgc
    15361 cggcctggcc agcggccacg cgctgggcaa cgtgccggtc gagacgctga tgctgcgcct
    15421 cgtcgtcacg cgcgagcgcc aggtgcggcg cctgccgtcg ttccacttcc tgtcgctgcc
    15481 caggtcgttc ggcaacggct gcagcgagtt cacggtcgaa ctgctcgacg cgtgccgccg
    15541 cacgctcgtc tgcgcgccgc tcgactgcgg ctgccagcgg gccggctgcc actgctggcc
    15601 gcgcacgatg cgcggtgcgg tgccctaccc ggcgtccgca cgctggctgc tggtctacga
    15661 gaacgaccgc aagatccacg aggaatgcat cccggcgcgt ccgccgcagg tcaggttcgg
    15721 cgagtcgaag cccggcgagc gcggcatgca gctgtcgtgg gagccggtgg cgctggagac
    15781 gctggccacc gactgctgcg aaccgtgctg cgacgccctc acctacctcg tgcactggta
    15841 cgacgaggag gacggcatct ggcgcggcat cgcgccgcgc ggcacggccc gcacggcgct
    15901 cgtcccggcg acgctctacc gccgcgccga cctgcgcgtg cgggtgctcg cctcgtgtgg
    15961 cgtcgccacc gggcatgccg agacgaccat cccgcgcggc ggcaagccgg gcggcggcga
    16021 aggcggcgac tgcccgcccg accggcgccg cggcgccggc acggcgtcgc tgtacctcga
    16081 aggctacgac gccaaggcgc gcggccccaa gcccggcacg caggtgctgc gcgccaccgc
    16141 gatcggtgaa ctgggcaagt cggtcgaggt cacgcgcgtg cgctggtacg gcgagcgcgg
    16201 cgaggagctc gcggtcggcc cgcagctcga cctgcgccgc ctgccgcgcg acacgcagac
    16261 catccgcgtc accatgcgca ccgccgacgg cgactggctg caccagtcct ggccgctcga
    16321 tcgcgcacgc cgctgaacac ctggaaggag acgagacatg cagatcatca acggatcggt
    16381 cacgttcggc cagcactcgg gctccggccc gcaggtgcag acgaccacgg tgaccatggg
    16441 tgcgcccgtc agccaggccg cggccttcct gagcggcttc aacgtcgagt tctcgcacgg
    16501 ggacgaccac aacttcgggc tgctggacgt gcaggtgcag gtcccctcgg gcggcatcag
    16561 cggcgcggcc gtgcaggtgc aggtgagcta cggcctgcgc gactggtcgg gcgactggga
    16621 cgacgactac gacgggcaga tcttcttcac cgtggtgggc gagtaggcgg cgccgctcac
    16681 gtcccgcgtc gtcgcattcg aagcgacacg atgacggcga cgatggcgac aagcgcgacg
    16741 ccggccgcca cgagggtgtc ggaaggcgac ctcgcttccg cggtcgcacc cgccgtcgat
    16801 gacggcgcgg cgccgtccga ggcgaatgga ctgaagccgc cctggcccgg gtcggacggc
    16861 gcggacgccg cggcgcgttc cgccgcaagg cctgccgggt cgagcgtcgc ccgcaggccc
    16921 gccagcgtgg gagcgttgcc ggtgccgaat gcccagtgca tgccgctcca cgcgacccgc
    16981 atgtcctcgc cgtcgcgccc cgggtcgagt gcctgttgct cgacgagctt gggcgtcggc
    17041 atggcgagcg accagatctc cgcggccggg tggtcgccgc gtgcgaccac gcaggcggcc
    17101 acggcgccgc cgtcgccgac cgccaggctg gtcgccgtca ggtcttgcgc ggcgtccgtt
    17161 cgctgccagg ttcctcgcgt gccgaatgac aggtccagcg agccgtccgc cgcgaggtgg
    17221 aacacgaccg cccgggtcgg ctcgacgttg gctgcgacgc cagccacgag cagcgatccg
    17281 tccgcgaggg gcagcagatc gttcggcttg atcgccaacc cgccaggcac gacctgcgcc
    17341 ttgccctgca cgccccaacg caggtcgggt gcgccatcgg gcaggtaacg caggatcacc
    17401 acttgcggct ggccgccggc gatgctggcg ccgctggccc agattcgccc gccgtcgtcg
    17461 acgcgaaggg cggtgggcgc gtcgtccgtg gcgctgatcg tgagcggccg gcgaccgcca
    17521 tgaccgaagt tcggatcgag ccggccgtcg gcgtccagat gctcgagcac gtagccctgg
    17581 gcgccgtccg ccggatcgct gaccgcgcgc cattgatcgc ctgcggcgtc gcgcaccgtc
    17641 aacgacccgc aatgcgccgg atccggcgcc agcagcgcga aggcatacgc cgccgcgaca
    17701 gatgcgaatc gcaccgcgtg gcgcaacttc gcgacggtca tcgcctgcgg ccaggacatg
    17761 cgactcaacg cgcggccgtc gcgtgcggcg cggcctcgcg gagtcgcgat tcgacggcgt
    17821 cggccgtgag gatgcccttc ttgcgcgagc cgtcgccgaa gaacagcgac ggcgtgaccg
    17881 agatgcgttg ctggctcgcg aaggcaacgt tgcgttcgag cggcgcgacg tcgcacttca
    17941 tcgcgcgcat cggctccacg cgccgcagca tccagtcgcg ccacgccctg gccggatcgc
    18001 cggcgcacca gatgtcgcgc gcccgcgtct tggactccgg cccgaggatc gggatcatga
    18061 aggcgtagat ggtcacgtca cggacgttga ccaggtcgcg ctcgaaggac ttgcagtatt
    18121 cgcagtacgg gtcgacgaac atggccatgc ggcgagcgcc gttgccttgc cggatcacga
    18181 tcgcgtcctt cagcggcagc ttgtcgaagt cggtgaccat gaaggcctgg ctgcgcgctt
    18241 cggtcaggtt ctgacgcgag tgcgtctcga caagcgaccc gttgacgaac acgtagtccc
    18301 cgttctcgct ggcgtacagg atctccgtgc cgttgtagcg cacctcgtag agcccctgga
    18361 cgggcgactt ggtgatctcg tcgatccgcg gcaggtccgg caagcgctcc gagagggcac
    18421 gtcggatggc cgcatcgtcg gcgaacgcac cgcaggtcgc cagcccgagc gccaccgccg
    18481 cgaaatggaa cgaaagagtt cgagcaatgg acatcgacgt cacctccgca tcgattcttg
    18541 cacccggccc ccggcgctcc gagtacgggg tagttccgac agggaaagtc cttggccaag
    18601 gtgggtcggc cgcgacaaac gccgcggctc cggggaaggc cgccgaattc gcgtgatggc
    18661 gcgaagcccg ccataccgca tttggggtat ggcggcgacc gcaacatccg gcgcaacccg
    18721 atgccgccgt cgcatcgcaa cggctaatgt gcaaagaggc cattctctga tcgagccgtg
    18781 gccgcccctt cgacatccca tccacccgag aggattgcgc catgaaattc gattcccgtc
    18841 gtcttgcccg tcccatcgtc ctgaccgcgc tggccgcggc gtcgttctgc gccctggccc
    18901 aggagtccgc caaggcgatg cccgacgcag ccgccaacgc cgcggcgccg cggggcgtgc
    18961 actggaagaa cgacccggat tccctggcga tgcccggatc ggtggccggc cagcgcgccg
    19021 ccgccgaagc ggcggcgcgc tcctcgacga tcgccgcccg cagctcgggc aagacgctga
    19081 cctactacgg cggcccgatc gtcaagaaca tcaaggtgat ccaggtgctg tatggcagcg
    19141 gcacctacca gcccttcgtc cagggcaccg gctcgggcac gatgtcggcg ttctacagcg
    19201 gcgtggtcgg cagcccgtac ttcacatggc tgaacggcga ctacggcacc ggcagcggca
    19261 agaccacgca gtcgatcgga tacggcagct tcgcgggcca ggtcacgatc acgccgtcca
    19321 gctcgcgcaa cggcagcacg atcaccgacg cgcagatcca ggccgaactg gccgcgcaga
    19381 tcagcgccgg ccacctcgcc gcgcccaacg ccaacacgta ctacgccgtg ttcttcccca
    19441 agggcaagac gatcagccag ggcggctcca attcctgcgt ctccggcggc ttctgcgcgt
    19501 accacggcac cgtggccggc agctccgcga cctggatgta cggcgtgcac ccggacaaca
    19561 gcgccggctc gggctgcgac gtgggctgcg gcggcagcac gcccttcaac aacatgagct
    19621 ccgtctcgtc gcacgagatg atcgagacgg tgacggatcc ggacgtcggc atcgcgacga
    19681 cctacgcctc gccgctggcc tggtacaacc ggacgtacgg cgagatcggc gacatctgca
    19741 atgcccagca gggcaccgtc gtcggaaccg acggcgtgac ctacaccgtg cagaaggagt
    19801 ggtcgaactc gcagtcgatc tgccggatcc agtgatccgg ctcctcgagt gagctcaagc
    19861 ccgcggacac gctcgcaggt gtccgcggga actccggcaa gcgccaccgc gccgcccacc
    19921 tcgtcctgcg tcccctgaag gacgagcacc ggcgcgccga gcacacgacg atgccggcca
    19981 gctccgcgcc gctgcggccg cagtcgagtg ccgccatgcc gccggcgtgt cccggacgca
    20041 ggcgctgggc gcgcctcagc cgcaggccgt cgccgccgcg ctgcgttgcg cgagccactc
    20101 gccgaacccg cgcggatcca tcgggcgcga gatgcggtag ccctgcgcca cctggtagcc
    20161 ctggccggcc agcagcgcca tctgcgcctc ggtctcgacg ccttcggcca ccacgatcag
    20221 gcccaggctg tgcccgatct gcaccacggc gcggctgagg gcctgcgcgg tgacgtcggt
    20281 ctcgaggtcg ctgacgaacg agcggtcgag cttgagctcg tggatgggca ggcgccgcag
    20341 gtagctcagg ctggagtagc ccgtgccgaa gtcgtcgacc gacagccgca cgcccaggcg
    20401 atggatctcg ccgatggtgc gccgcgtggc cgggtggtcg tcgatgagcg tgccttcggt
    20461 gatctcgacg accaggtcgg gcggcgccag gccacgctcg gacagcatgc ccaccagccg
    20521 ctgcggcagc cccaggtcgt ggaagctggt ggacgacagg ttgaccgaca cggacggcac
    20581 cacgaggcct gcggcgcgcc acgcggccag ctggtcgcac gccgtgcgca gcgcccagtc
    20641 gcccagcacc gcgatcaggc cgcagtcctc ggccagcggg atgaagcgcg acggcggcac
    20701 gttgcccagc tcgggatgcg accagcgcgc cagcgcctcc gcgccgcaga gctcgccggt
    20761 gcgcaggtcg acctgcggct ggtagtgcag cgagagtccg ttgccctgca gggcggcgcg
    20821 cagcgcggtc tccagggcca ggcgctcctg cgcgagcgcg ttcatctcgg cgctgaagaa
    20881 gcagtagctg ccgcggctgt cggtcttggc ctggtacatc gccatgtcgg cgcgcatcag
    20941 gagcgtctcg atgtccacgc cgtcggacgg gaacaacgcg atcccgcaac tggccgacgc
    21001 gttcaggcgc tggcccgcga tctcgtgcgg atcggcgatg cgcgcctgca gccgttcgac
    21061 gacgacggtc gagtcctcgg cgtcgcaatc gggcagcacc agcacgaact cgtcgccgga
    21121 cagccggccg gcgatgtcga ccgcgcgctt gtcgcgcgtg atctggcgcg cgacgtggca
    21181 cagcagctcg tcgcccgccg catggcccag cgaatcgttg acctgcttga agcgatcgag
    21241 gtcgatgaag atcaccgcgg ccggcgcgcc ggtgcgggcc gccgcggcga gcgcctgctc
    21301 cgccttggcc agcagcaggc tgcgattggg cagcgcggtc agcgcgtcgt agaaggcgag
    21361 ctggcggatg cgcgccttcg accgctcgcg ctcgaacgcg agcgcgcaca ggtggatgca
    21421 cgcggccatg agccggcggt ggaactcgga cggcgcatgc gcacgcgtgt agtggaacgt
    21481 gacggtgccc agccgcgtgt ggtccaccgc gttgacgggc atcgtccagc acgccgcgta
    21541 gcccatgtcc agcagcgcgg cgcggaactc gggccagggg aaggacgggt cgccggcgat
    21601 gtcggcgcac accaggccgt cggccgacca cgcggcctcc tgcgcgggcg agccgatcgg
    21661 ccagcgcgcc ttgtcggcca cgcgcaggcg gtaggcctca ggcatgctgg gcgccgcaag
    21721 cggcagcagg cgcccctgtg catcggcctc ggtgatgaag gtggcgatgc cgggcaggat
    21781 gccttcgacc tcggcgcaca tcatctccat cacctccacg agcggccgct cgcgcgcgat
    21841 ggcctcgagg acgcggtgct gcagcacctc ctgcatcttg gactgggtga tgtcggtcag
    21901 cacgctcacc gcgttgacga ggcggccccc ttcgtcgaac accggatcac tggtgatgga
    21961 gcaccagtag cgcgcgccgt cgcgtccatg gaggatctcc tcgcgccgca gcgacgtgcc
    22021 cgcgcgcagg ctgtccagga cggccccctc ctcgccctcc aggtgcggcg cgagcagtcc
    22081 ggccgggcgg cggccggcga tctcggccag gcgccagccg aacatgcgcg tgaagccctc
    22141 gttgacgtac gtgatgcgcc gatcggcgtc ggtgatcagc acggcgttgt cggtgacgcc
    22201 cgcgaccatc gacagccgcc ggatctcctc ggtggcccgg cgctcgcggc cgacgcgctc
    22261 ggtgacgtcg ctggccatct tgacgatgcg cagcacctgg ccccgcgcgc cgagcaccgg
    22321 cgcgtacgtg gcctcgagcc agcacgcctg gccgccatgg cgccggcgtt cgcacaggtc
    22381 ggtatgggcc tcgcccgcgc gcagccgctg ccacagggtc gcgtactcgg gcgatgcgac
    22441 gtacgccgga tcgcagaaca tgcgatggtg cgcgccctgc acctcggaca attcgtagcc
    22501 gaagatctgc aggtagtgcc gattggcgtg cagcagctcg ccatcgggcg agaactccgc
    22561 gaacgccatc accgcgtcca gcgtggccag gttgcggtcc agcaggtgat cggacgggga
    22621 agcgggttgc attcttgtct ggggatctcg gggcgacgag ggcgacgggg ccaggcgcag
    22681 cgcctcgccc gagttcactg ccgctcgtct gttgtcgacg ctttccgaga ctacttgatg
    22741 ccatcgcggc cgggaacggc ggacgtcacg aactcccctc accaatccga ccgactccgg
    22801 gcattacgca cggccgccgc cgccgtgcat gccggacgtt cgctcggcaa gggcgcgatc
    22861 cgcgtcaccg cggctcggcc cggaccggct cgtgcgcgac gctcgaggac atcgcctcga
    22921 tctcctcggc ggggaccggg cggctgaaca gatagccctg gccgtggtgc acatgcaggc
    22981 gccggagcac ggccagctgc tggggcgtct cgatgccctc ggcgacgatc tccttcttga
    23041 gcgcgctcgc caggcccacg atcgtggcca cgatggcgta gtcctcgccg gtgctgctca
    23101 ggtcgttgac gaagctgcgg tcgaccttga tcttgtcgat cggcaggcgc ttcaggtagg
    23161 acagcgacga caggccggtg ccgaaatcgt cgatggcgat cttcatgccg cgcagcttca
    23221 ggatggccag catctgcacg ttggcggcca cgtcctcgat gagcgcgtgc tcggtcagct
    23281 cgatctgaag ctggcgcgga tccgcgccgc tgtccttcag ggccgcgtcc acctggtcga
    23341 tcaacgtgcg ccgcgccagc tgctgcgcgg acacgttgac ggcgatgaac aggtgcccga
    23401 ggccgcggcc ctgccaggcc gcctgctggc gcgccgcggc gcgcagcacc cagccaccga
    23461 gctccacgat caggccggag ccctcggcca cctcgatgaa ggcgcccggc atcagcaatc
    23521 cgcgcgtcgg atggcgccag cgcacgagcg cctcgacacc gatgaccgcc tcggtcccga
    23581 gatcgaggat gggctggtag tgcaggacga gctcgtcgcg ctgcagcgcg gcggcgagct
    23641 cctcgctgag ctgcaggcgg gcgacgttct cgcgatccat gctctcgtcg tagatcgcga
    23701 agccgttctt gcccgaggac ttcgcgcggt acatcgcgat gtcggccgcg cgcagaagct
    23761 cctgcgccgt ggcgccatgg cccgggaact cggcgatgcc cacgctggcg tgcaccggca
    23821 tgagcttgcc gcggaagcgc atcggctggt tgaagcaggc gatcgcatga cgacccaggt
    23881 gctcggccga ttcgccggcg cggcgcgagc ggatcacggc cacgaactcg tcgccgccca
    23941 ggcggaagac cttgccggtg gcgccgaacg cctgctgcaa cgcgccgccc agctggccca
    24001 gcagcgcgtc gccggccgca tgccccatcg tgtcgttgac cagcttgaag tcgtccaggt
    24061 ccagcagcag caccgagaag cgggtcgacg gctcgccggc gagcccggcc agcagttcca
    24121 tcgccgcgtg ccggttctgc aggcccgtca ccgggtcgaa gtacgcgagc tggttcagct
    24181 cgtcctcggt ccgggcgacg gcccggcgca cgcgccgcac gcgcacgtag gagaccacca
    24241 ggcccagcag cgcggacagc agggtcgcgc cgcacagcat cagcgtctgg cgctgcaggg
    24301 cgcgccggga cacgaccagg cgcagctcgc cgagacgctc gcgcccctgc atcaccggct
    24361 cgcggacctc gatggcgccg ggctccgcgg gaagcttgcg cgcggcggcg tcgggcgcca
    24421 ccgcgtcata gcgggcgaac acctggccgg tgacgtccag caccgtcgcc tggcggatgt
    24481 ctggcgagct cttgagcagt tgcaggatct cgcccgcggc gcgcgcatcg cggaacagca
    24541 gcgcgggggc gatgccgtcc gaagcgatgg ccgcctgcag ttccgcctgg ccgcgcagcg
    24601 tcgcatgcga ccagccatag accagcacgt tgaccgccag tcccgcgacc agcagcgcga
    24661 gcgccgtcga ttgcagggcc gcgatcgtga ggcgcgcggc cagcgatcgc gggcgcggcg
    24721 cggatcggcc aggcgggagg acggcgggcg acgtcattcc gaaaccgtcc tggccaggcg
    24781 cagcaggcgc gagctgatgg acaggcccga ctgcagcgcc ttgcgctggt cgatgtcgaa
    24841 cgcgagccgg ccgtcggtga acacgagctg gatggagggc ctggccgcgg gacgatagtc
    24901 gctcgaaccg atcacgagcg ccggctcgcg cacggcgttg cgcgcgtcct gttcggccgc
    24961 cgcggagtcg acgtagaccg cctggcacgg gtgcggggcg ccctcgagcc cggccacgtg
    25021 cagcgtcatg cgccgcacgg cgcgcccttc gagcaggcgc accggcgcca tcatcggact
    25081 gtcgcttcgc aggcacagcg tgagcgccga gccgtcggcc agcgcgctct cgttcggcca
    25141 ttcgacgaac tgcaggacgt tgaacacgat ggcggcctcg agcgcaccgc gctccacggc
    25201 caacgcgccg cgcagcggca gcagcgcgag cccgacgccg gccagccacg cgggccaggc
    25261 tcgccgcaac gaatggagtc cgttcatcgt cagaacgcct tcgagacggt gatctcgatg
    25321 ctgcgcccgg cctgccgaat ggaaggttgc gcctccaggt cggtgcccgg atcgtcgtat
    25381 cggcgatcga acaggttctg cacgttcgcc gaccacgacc agccgccggc cgccgtcgtc
    25441 gacgacagcg cggcgttggc cagcgcatac gcgcccgcgc cgccgcggcg cgcgacggcg
    25501 cgccactgca gccccgcctg cacgcccgcg gccagcggca gcatcgccgt caggttggcg
    25561 atccagtgcg gcgcgtagac ggcgatcggg gtgccggtgg cccggtcgtg cgcgaaggac
    25621 gtgctgacgt tggcgcgcag gcgcgggccc tgcgcccacg cgtgctgcca ctccacttcc
    25681 gcgccgcgcg ccacgacggt gccctggttg acgaaggtgt acctgtcgtt ggcgtcgtcg
    25741 cgcgcctgca cgatcaggcg ctcggcgcgg ttctcgaaga gggatccgcc caggctgtcg
    25801 ctcgcgtcgg gatgccactc gacgttgagc tcggcgccgc gcaccgcctc cggtcgcagg
    25861 cgcggattga cctcgtagcc gccggagccg gcgatctggt agtgggcctc gaagtcgttc
    25921 ggcggccggt aggcggtccc gacgatggcc ttggtcacga gcgtgtccgc gggccgccag
    25981 acgagcgcca tgcggccgct cggctgcgcc cggtagccgc gcgtcgcatc cagccgcagg
    26041 ctgccgtcga ccgacacggt gcccgtcgca cgccagacgt cctcggcgaa gacggccagg
    26101 cggcgcgacg ggtcgttgcg atccaggtag ggctcgctcg ggggcgcgac gtccgcgttg
    26161 aacgagcgca gcatcggcga gagctggaac tccgcgccgg ccgtcaggcg atggccggcg
    26221 atgcgctcgg tggtgacatg gccctcggcg ccgacccagt gggccgtgtc ggtgtcctcg
    26281 ttgatcgtca cgggcgccgc gtccatgacg tagcggccga cgaagtggta gctcccgccg
    26341 tacaggcgca gcgtcgactg gtcgcccgcc tcgctcgagc ctgtccaggt gaggtcgtac
    26401 aggccctggt tgtcgatgtt gcgcgtgcgc cagtcgcccg ccagggtatc gacgatggcg
    26461 gggttgccgc gcgtgcgatg cgactggatc agcgagagcg ccaggccgcc ataacgcgcg
    26521 ttcagcgtca cgctcgtgcg ctgctcgaag tcgccgcgcg gcgtcacggc gccgtcggcc
    26581 ggcacatcct cgccgcgcgt cccgcccgac gaggccgaca gccgcacgac cgcgccgccg
    26641 gcggccgaca gcgtgtcctc cagccgcacg cggcgttcgc cgaacgagcc gagggtcgcc
    26701 tccgccccgg cgggcgcgcc cgcgtcgggc cggcgggtga cgacgttgac gacgccgaac
    26761 agcgcgttcg cgccgtagac ggccgacgcc tgccccggaa tgaactcgac gcgatcgacc
    26821 agcgccatgt cgaccgggaa ctcgctgccc aggtaggcct ggtcatagag cgcgtcgttg
    26881 gcgcgcacgc cgtcgatgag cagcagcacc cgcgtgttgt agtcgccggg cgcgtagaag
    26941 ccccgcacgc cgatgtagct gtaggagcga tcggtcgtga ccatcgcgcc cttgaccgtc
    27001 gccagcacgt cggccagtgt ccggcagccg agcgcgcgga tctcggcggc gctgacgacg
    27061 gtgaccgcgg ccgcggtgtc ggcgagcgtc gacgggaact tcgagacgcc cgagacctgc
    27121 atgtcgagca gttggtccag cggcatcgac gcgaggtcgg gcacgctcgg gttggccgcg
    27181 agcgcggcgc tggaggccag gcccaggctc agcgacaagc aagggacgag aagcgacttc
    27241 ttggacatgg aacgggtacg gcgagaaccc gtcttcatcg accgaaggac cgccttctga
    27301 agccgggaca acgttgtcct gcgcacttcg ccacgacccg acaacactga tcggacgttc
    27361 ccgctcggca gcctcgtacg tgcgctccca cggcgcatgg cgcggcgtcg gcgccgcaaa
    27421 tgggactggc acacatattg cccgacagta ggcgaaacga ccgatcaggt ttcgccatgc
    27481 cggcccagga cgacaaactg cgggtgctgg tcgtcgacga caacgtcgac gccgcggcga
    27541 cgttttgcgc cctgctgcgc ctctcgggct gcgaggccca ggccgcctcc gggctggcga
    27601 tcgcgctggc cgtggcggcg tcgttcgagc ctgacgtcgt cgtgctcgac ctggagatgc
    27661 ccggtgcgga cggctgccgc gtgctgcagc aaatgcgcga acgcggcgac ggacttgccg
    27721 atccgctgta cgtctgcctc accggggcga gctccgacga gaaccgccgc cgctgcgagg
    27781 cggccggctt ccaccgcttc cacgccaagc ccctgcgccc ccatgaactt gccgaaatcc
    27841 tggccgacgc acgcgcgcgg cgcctgcggc agcgcgtcgc catggccatg cccgaggccg
    27901 cgccgctccc tgcaccgggc gccgcgatct cgcagggcgt tcgagcggga ccggcaggca
    27961 atgacccgct cagccattga aaagtgcagc tcgcacagtt cttgcccgta ccggaacgga
    28021 cggcttttcc tccctgggcc gtcccttcct tgaagcactt tgagcccggc ccccatgtcc
    28081 gggctctttc ttttgcccgg cccggccgca gcctgacggc gtgcgcgtcg tgactccgga
    28141 tccgacgccc agggtcgatc cggtcgtccg cgacctgtcg ttcagggaca atgacatgcg
    28201 tgcggcgaac ggactgtacg ctcgttcagc caagaggacc cggactcaat gcgtgaccta
    28261 ggtgatgtgt cgctgccgcc cgacgacttc agcgcgatgg cgacgctctg caagcacgac
    28321 caggatttcc ccccgaccct cgaagcctgg cgagccatga tctccgaggc cacgctcgaa
    28381 gcccaccgcc gtgggcagta cccggcgccg atgcagatcg atgtccgcaa gttctcggac
    28441 tggtgctcgc tcgtgcaggt gctgccctgc ctcgattcgc tgcgcgcgtt cgtgatcatc
    28501 cggcgccgcg agatctcgtg agcgcgccgc gccccgagac ccgccctttc ttcgatgacg
    28561 tcgcggcctc cgatcgatga cgagttgagg cgcttcgtcg tcgccagcgt cccgagcgtc
    28621 gcctggctgg aggcgatgct gctgttccgg cggcaggcgg tgccgcgcac gatcgccgag
    28681 gccgcgctcg agctctacat cccggagccc gcggccgccg acgtgctgcg cgcgctggtc
    28741 gaggcgggcg tgctgtcgca ggcgggcgag acattcgact acgcgcccgc gaacccgcgc
    28801 ctggcagcga tgctcgaccg ggtcgccgcg gcccatgccg ccgacctggt gggcatcacc
    28861 aacctgatcc acgacaagac gcaccggaac gcccgccgct tcgcggacgc cttcaagctg
    28921 agaaaggacg cctgaatggc ctcggcgatc tacaccctct gcgcggtcac ggcgttcgca
    28981 tgcgccttcc tgctcctgcg cagctacgcg cgcacccggt tcaggctgct gctgtggagc
    29041 ggcctgtgct tcgcgggcct gtgcgccaac aacgtgctcc tcatcctgga tcgctacgtc
    29101 ttcctcgcgg tcgacctctc ggtggcgcgg caggccgtgg ccttcgtgtc ggtggcgctg
    29161 ctggtcgtcg gcctggtact ggagagcgac gcatgaaccc ccaggacatc ccgacgatgt
    29221 tcgcggtcaa cgaactgctg ctggggggca tcgccgtcgg atgcgcgatc gccggactct
    29281 tcttcctgcg ctactggcgc agctcgaagg accggttctt cctgtgcttc gccatctcgt
    29341 tctgggccga ggcggcgaac cgcctgcaca tgggcctgac cgccgcgtgg aacgagacgc
    29401 agccttcgca ctacctggtg cgcctgctct gctacggcct gatcctgttc gcgatctggg
    29461 acaagaaccg gccgggccgg gactgacgcc ggtcaggccg gcgccggtcg ggcgaccggc
    29521 tggatcgcgg ccagcgagct cagcacgatc tccgcctcgg cgacgagatc cttcggcgag
    29581 gcaacgcccg cggtcagctc gcgcatcggc gggctgcccg tctcgaacag cagctcgacg
    29641 tcgttgcaca gtgcgcgccg ctgctcgatg tccatcgcgt cccggagcac gggcacgagg
    29701 cggtgctggt cgagcaggtg ctgctgctcg acgtgctcgg cgaacgccgc cagggcctgc
    29761 gcatgcccgt ccgaggacgg cgggcgcacg aggagctcgg ccagcgcctg cttgaagcgc
    29821 acatggccgg tcatcgcgtc gagcggcagg tccttccagc ggatgtggcg ccacgccggc
    29881 aacaggacgc gctcgcgcga cagcacgtgc agcaccgccc ggttggcgag gtcgccgaac
    29941 aggcgcaggg cgtcctcgcc gcgcctgctc gatcgaaggc gcgagatcag tgccgtgagc
    30001 tcggccagct ggcgggtgag gagagcgctg cagtccacgg cgacaagtcg gtttcagccg
    30061 ccgcggcggc aatgggcatg ccttcgcgtg ccgacgagca ctcgagcggc ggctggcggc
    30121 ctggagcggc atgaaacggc gcgttccggc tcggcaggcg tgatcgaggg cgctcgcgac
    30181 gcgtcgccgg gggtgccgcc accggcaagg cctgctcgtc ttcaggcggc gatccttgcc
    30241 gcagcaggct gcacctgccg cgcagccctc cttgccgccg acgccggccc cgagcgctcg
    30301 gccggaaggt tgaccagacc ggtcgcgacg caacgctcga cgagaatgac cgccgcgctt
    30361 cgcaccgtcc gcgagcggcc cgccactcac cacgatgacc tcttccccgt ccgccgctgc
    30421 cccgggcagc caccgctcct cgtgggcgat ccatctcaag ctggtcggca tggccgcgct
    30481 gtggggcggt tcgtggcctt gcggtcgcgt gctggcccag gcgctccctc cgctggcggc
    30541 cgcgacctgg cgcttcgcca tcgcgctcgt gctgctgctg gcgtggatgc aggtcgacgg
    30601 cggcttcggc cgcctgcgca agctgacgcg gcgccagtgg ggcgggctgg ccgtgggcgg
    30661 cgcggtgggc gtgttcggct acgcgatgtt cttcatgctc gggctgtccc atgtcgccgc
    30721 cggccgcgcg gcgctggtgg tcacgaccaa ccccgtcgtc acgaccctga tcgccgcctg
    30781 gtggttcggc gagcgcctga actggcgcat cggcgccggc atggcgctgg cggccgcggg
    30841 cgcgctcatc gtcatgacgc acggcgctcc atggacgctg cttgccggca gtgtcggcaa
    30901 gggcgaggcg ctgctgctcg gatgcgtcgc cacgtgggtg gcctacacgc tgatcggccg
    30961 ccggctgctc gccggcatcg acgccctcac gacgaccacc gtcacggcgg ccttcggcct
    31021 cgtcatgctg gccgtgacgg cgctcgtgtt cgaagggccc gccgcggtcg cgaccccgct
    31081 gaccgcgagc gcgacgacct gggtcgccct gggcttcctg gccgccggcg ccacggtcgt
    31141 cgcctacgcc tggtacttcg aaggcgtggc ggcgctgggc gccggcgccg ccgcgggcta
    31201 catcagcctc gtcccgttgt tcggcgtgct gtttgcgacg tcgtggctgg gcgagccgct
    31261 cgacgcatcg atcgtcgtcg gtggcgcgct ggccgtcatc ggcatggcgg tcatgaacct
    31321 ggcgcgccgc tgatcggatc cgacgccgaa ccctctcgac cgtcccggcc cgcctccgcc
    31381 tcgaccgccg caaggtacgc ccggcccagg ccgctcagct gaacgtccag gggcccgtgc
    31441 agttccagca ggcccatcgc catcaggaac gccacctcgc ggagcaagcc cggccgcagc
    31501 ggctcggtcg agagcccgac accgtcacga agccggcgga gcacctccac ctgcgggggg
    31561 gtcagcgcgc tggccatgca cgcgccctcc tgctcgtccg gatctggcca gcgcggccga
    31621 ttgcgacgac gcccatgcgc ctcctcggaa ctcagaggcc gaccattgcg ctggcccagc
    31681 gcgcgagcca gctgtcgaac acgagccgtc cgtcgacggc ggccaggcag atgcactcgc
    31741 cctgcgcctg cacgacgggc tggtgcagca cgtcggcatc cgcctcgtcg aagtcgccgg
    31801 cgctgaacac ggcgcggccg tcgtcgaacg agccatgcag gacctgggtc agctcggtcc
    31861 cgccgtgcgt gtgcaacggc aggcacttgc ccgcgccgat gcgcagcagc agcacgttgg
    31921 cgtcgcggtc ctggggcacg gtcacgcggc tccagcgcat gccggggccc atcgtgcgcc
    31981 agggcgagac ctggcagtgg cgcagggcgc gcggccagtt cgacccggcc ggcagcggcg
    32041 gcttgggacg cacgaccggc ggcggcggga cggccagcgc gccaccgacg acgatcgatg
    32101 cgtcgagcgg ctcgcccagc cacgacgtcg caaacagcac gccgaacaac gggacgaggc
    32161 tgatgtagcc
//
LOCUS       DATCRO010000731         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_874282, whole genome shotgun sequence.
ACCESSION   DATCRO010000731 DATCRO010000000
VERSION     DATCRO010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874282"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIN75_19800"
     CDS             complement(<1..779)
                     /locus_tag="VIN75_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8976470.1"
                     /translation="MTSNQASPRALQPTRVEYSERRLTSMNGYAMVLLAVVLVVVALT
                     RFVSIRDDMEAASVLLAHVLPGLLCVLATVAVLAGLYTLQPNEAAIVTLFGQYAGTDR
                     AEGLRWANPFFVKRRISLRARNLNSPALKVNDKRGNPVEIGAVIIWRVEDTAKARFEV
                     DDVDLYVKLQSEAALRHVASLYAYDEADSEDAGEITLRGGLDDVAQALGDELSARFAS
                     AGVVVSDARLTHLAYAPEIAQVMLRRQQAEAVISARHKIVQG"
     gene            964..>2130
                     /locus_tag="VIN75_19805"
     CDS             964..>2130
                     /locus_tag="VIN75_19805"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976471.1"
                     /translation="MPQHRARAPKRIALAIATAAVATLAACAGGGARPGVVNGEVVTP
                     VAPGLRGYPFAASRTTTQDYFGSAISDDFGWLENAGDPYTRSWIAAEHAYGRRYLDAM
                     PARAELRQRLKALLESGTNAYSSLVERGGLVFALKSAPPAQRPLLVALKSLDDLGSER
                     VVFDPNVLAPDGSVSIEFFRPSPDGRRVAIALSAGDASTAVVRVVDVLTGQVLPGEVA
                     RVLSAGAGGDVAWSAGGNGFFCTQVASPGAPALVQVVFHRLGATGTDRVELSAGLPRL
                     ARVRLESARDGKHVAALVEDGRGGDVALFVKPSDANGEGAWRRLASGTDGVRDAQFGD
                     DDALWVHAIGNAPRGKILRVPLSDTRPVAWDRLAAVAMPVDGALQRFALAGGTLY"
BASE COUNT          272 a          748 c          808 g          302 t
ORIGIN      
        1 ccctgcacga tcttgtggcg cgcgctgatg acggcctcgg cctgctggcg gcgcagcatc
       61 acctgcgcga tctcgggggc gtaggccagg tgcgtgaggc gggcgtcgga gacgaccacg
      121 ccggcgctgg cgaagcgcgc cgagagctcg tcgcccagcg cttgcgccac gtcgtcgagg
      181 ccgccgcgca gcgtgatctc gccggcgtcc tcgctgtcgg cctcgtcgta ggcgtacagc
      241 gacgcgacgt ggcgcagcgc ggcctcggac tgcagcttca cgtacaggtc gacgtcgtcc
      301 acctcgaagc gcgccttggc ggtgtcctcc acgcgccaga tgatgacggc gccgatctcg
      361 accggattgc cgcgcttgtc gttgaccttg agcgccggcg agttgaggtt gcgcgcgcgc
      421 agcgagatgc ggcgcttgac gaagaacggg ttggcccagc gcaggccctc ggcgcggtcg
      481 gtgcccgcgt actggccgaa cagcgtcacg atggccgcct cgttgggttg cagcgtgtac
      541 aggccggcga ggacggccac cgtcgccagc acgcacagca ggccgggcag cacgtgcgcg
      601 agcagcacgg aggcggcctc catgtcatcg cggatcgaga cgaagcgcgt gagcgcgacg
      661 accaccagca ccacggccag caagaccatc gcgtagccgt tcatggacgt gaggcggcgc
      721 tccgagtatt cgacgcgcgt cggttgcagc gcgcggggcg aggcctggtt cgaggtcatg
      781 gaagctcccg gtgggtatca aagtgatatc actctacggc cccgctgccg ggttgtcaat
      841 cccgccgcgg cgtgcggtgg ttcgcacgtt ttgcaacacc ggcgcacttg gggcggggcc
      901 gccgccgcct aacatcgcgg tcggatcgat tccagcacca cgagggagac acgcttgaac
      961 gtcatgccgc agcaccgcgc ccgggcgccg aaacggatcg cgctggccat cgccaccgcc
     1021 gcggtcgcca cgctggcggc ctgcgcgggc ggcggcgcca ggccgggcgt cgtgaacggt
     1081 gaggtcgtca ccccggtcgc gccggggctg cgcggctatc cgttcgccgc ctcgcgcacg
     1141 acgacgcagg actacttcgg cagcgcgatc agcgacgact tcggctggct ggagaacgcc
     1201 ggcgacccgt acacgcgctc gtggatcgcg gccgagcacg cctatggccg gcgctacctc
     1261 gacgcgatgc cggcgcgcgc cgagctgcgg cagcggctga aggcgctgct cgaatcgggc
     1321 accaacgcgt actcgtcgct cgtcgagcgc ggcggcctcg tgttcgcgct caagtcggcg
     1381 ccgccggcgc agcgtcccct gctggtcgcg ctgaagtcgc tcgacgacct cggcagcgag
     1441 cgcgtcgtgt tcgacccgaa cgtgctcgcg ccggacggct cggtgtcgat cgagttcttc
     1501 cggccgtcgc ccgatggccg ccgggtcgcg atcgccttgt cggccggcga cgcgagcacc
     1561 gccgtcgtgc gcgtggtgga cgtcctcacc gggcaggtgc tgcccggcga ggtggcccgc
     1621 gtgctgtcgg cgggcgcggg cggcgacgtc gcgtggagcg cgggcggcaa cgggttcttc
     1681 tgcacgcagg tcgcgtcgcc gggcgcgccg gccctcgtgc aggtggtgtt ccatcggctc
     1741 ggcgcgacgg ggaccgatcg cgtcgagctg tcggccggcc tgccgcggct ggcccgcgtg
     1801 cgcctcgaga gcgcccgcga cggcaagcac gtggccgcgc tggtcgagga cggccgcggg
     1861 ggcgacgtcg cgctgttcgt gaagccgtcg gacgcgaacg gcgagggcgc ctggcgccgc
     1921 ctggccagcg ggaccgatgg cgtgcgcgac gcacagttcg gcgacgacga cgcgctgtgg
     1981 gtgcacgcga tcggcaatgc accgcgcggc aagatcctgc gcgtgccgct gtcggacacg
     2041 cggcccgtcg cgtgggatcg gctcgccgcc gtcgcgatgc cggtcgacgg cgcgctccag
     2101 cgcttcgcgc tggcgggcgg cacgctgtac
//
LOCUS       DATCRO010000732         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_885969, whole genome shotgun sequence.
ACCESSION   DATCRO010000732 DATCRO010000000
VERSION     DATCRO010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885969"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..840)
                     /locus_tag="VIN75_19810"
     CDS             complement(427..840)
                     /locus_tag="VIN75_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976472.1"
                     /translation="MSINHADIESCRAGIAAQLQAYRDLVGAASKASGMSLVRIDAAL
                     AAFEPAFFGNLLVALDARFASRPRGAGASAEVALIADALLHHGGVLTRNAALPHDADA
                     SVLRIDLGERVALNADDVETLAAAFLSELDGQATG"
     gene            complement(851..1321)
                     /locus_tag="VIN75_19815"
     CDS             complement(851..1321)
                     /locus_tag="VIN75_19815"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8976473.1"
                     /translation="MADPDTDARIEAFVAKYTPAIATQLREARQRLRARFPRGVEMVF
                     DNYNALVFGIGPTDRTRESFISIAGYPRWVTLFFLDGAGLDDPSGLLEGDGRQVRGIR
                     LKTPADLDSPAVAALIAQAVAPHAAALAAAPPLSTVIKAEAGKQRPRRPASAPA"
     gene            complement(1324..2316)
                     /locus_tag="VIN75_19820"
     CDS             complement(1324..2316)
                     /locus_tag="VIN75_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8976474.1"
                     /translation="MQRRSLLALAAGALAPSAAVVSTAARAADLYPNHPLRWIVAYPA
                     AGGSDFLARQLAPQMARQMGQTIVVDNRPGAAGIIGTEAASKAPGDGYTLLTGDNGAM
                     VFHTALYKSLPYNPSDFVPIGFMASFPLILAVNPSAGFTNAAQFLDAVKRNPGKYSYA
                     SPGAGSPHHLAMELLKQRTGSFIVHVPYRGTAPAMQDVMSGQVPIMVIDTAAGLTQIR
                     SGKLKALAVMSRHRIPQLPDVPTLSEAGGPALKDFEAEAWQGLFAPKGTPPDIAARLT
                     AEMRKAILTPEVKSRLEGFGLEVAPTDGPALTAFIQKETAFWHALIRERHLTLD"
     gene            complement(2464..>3014)
                     /locus_tag="VIN75_19825"
     CDS             complement(2464..>3014)
                     /locus_tag="VIN75_19825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY8976475.1"
                     /translation="ATQYRIARPARHRSGPQSRSLVHRPMPDFHPLSSPSFQPSCELC
                     REAGGVLVFQSAQWRVIRAEDPAFPAFYRVVWRAHVAEWSDLTMAGQAAMMGAVGTVE
                     RVLRDALAPTKINLASLGNVVPHLHWHVIARFDWDSRFPAPVWAAPVREVAPAPAARL
                     RTPLPELDARVARAVAALAPAA"
BASE COUNT          417 a          998 c         1179 g          420 t
ORIGIN      
        1 gcgtggggat gaggttgtgc agcaggtgct gcagcggcga tcgatgattc atgaaggcct
       61 cgcagtcgat ggaaggcgat ggttgcgcga cgcaggttgt cctcccggcg ttcgcgacgg
      121 aggccggcac ggggtcggga accgttgaaa ccattgcagc acatctgctt acattcgagt
      181 gccgggcgaa ccgcacgctg cagcgccggc cgtgatcttt gaccgcctcc gggtgcccgg
      241 ttttggggag gggccgagat ggcggcggcc cgctcgcaag cgctcgagga cgacggcgac
      301 gaggccgtcc tgttccggcc gcgtccggcc gatgtcgaag cgggtgtcgt gcatggcgac
      361 gaccgaaggc gggtccgtcg cgcatgcagc ccggccatcg ccgggctcga cttcgcggac
      421 ggctgctcag ccggtggctt ggccgtcgag ttcggacagg aacgccgcgg ccagcgtctc
      481 gacgtcgtcc gcgttgagcg cgacccgctc gcccaagtcg atgcgcagca ccgacgcgtc
      541 ggcgtcatgg ggcagggcgg cgttgcgcgt cagcacgccg ccgtgatgca gcagcgcatc
      601 cgcgatcaac gcgacctcgg cgctcgcgcc cgcgccgcgc gggcgcgacg cgaagcgcgc
      661 gtcgagcgcg accagcaggt tgccgaagaa ggccggctcg aacgcggcca gcgccgcgtc
      721 gatgcgcacc aggctcatgc ccgacgcctt tgaggcggcg cccaccaggt cgcggtaggc
      781 ctgcagctgc gcggcgatcc cggcgcggca ggactcgatg tcggcgtggt tgatgctcat
      841 gcgcgcattg tcaggccggg gccgacgccg gccggcgtgg ccgctgcttc ccggcctccg
      901 ccttgatcac cgtggacagc ggcggcgccg ccgcgagcgc ggccgcgtgc ggcgcgacgg
      961 cctgcgcgat caatgcggcg accgccggtg aatcgaggtc cgccggcgtc ttcagccgga
     1021 tgccgcgcac ctgcctgccg tcgccctcga gcaggcccga gggatcgtcc agtcccgcgc
     1081 cgtccaggaa gaacagcgtg acccacctcg gatagccggc gatcgagatg aaggactcgc
     1141 gcgtcctgtc ggtgggcccg atgccgaaga cgagcgcgtt gtagttgtcg aacaccatct
     1201 ccacgccgcg cggaaagcgc gcgcgcagcc gctggcgggc ctcgcgcagc tgcgtcgcga
     1261 tcgcgggcgt gtacttcgcc acgaaggcct cgatgcgggc gtcggtgtcc gggtcggcca
     1321 tcgtcaatcc agcgtcaggt ggcgctccct gatcagcgcg tgccagaacg cggtctcctt
     1381 ctggatgaac gccgtcagcg ccgggccgtc ggtgggcgcc acctcgaggc cgaagccctc
     1441 caggcgcgac ttgacctcgg gcgtgaggat ggccttgcgc atctcggcgg tcaggcgcgc
     1501 ggcgatgtcg ggcggcgtgc ccttgggcgc gaacaggccc tgccaggctt cggcctcgaa
     1561 gtccttcagc gcggggccgc cggcctcgga cagcgtgggc acgtcaggca gttgcgggat
     1621 gcggtgcctg gacatgaccg ccagcgcctt gagcttgccg ctgcggatct gcgtgaggcc
     1681 cgcggcggtg tcgatcacca tgatgggcac ctggccgctc atcacgtcct gcatcgcggg
     1741 cgcggtgccg cggtagggca cgtgcacgat gaagctgccg gtgcgctgct tgagcagctc
     1801 catcgcgagg tggtgcgggc tgccggcgcc gggcgaggcg tagctgtact tgcccgggtt
     1861 gcgcttgacg gcgtcgagga actgcgcggc gttcgtgaag ccggccgacg ggttgacggc
     1921 caggatcagc gggaagctcg ccatgaagcc gatcggcacg aagtccgacg ggttgtacgg
     1981 cagcgacttg tacagcgcgg tgtggaacac catcgcgccg ttgtcgccgg tcagcagcgt
     2041 gtagccgtcg cccggcgcct tggacgccgc ctcggtgccg atgatgccgg ccgcgccggg
     2101 ccggttgtcg acgacgatgg tctggcccat ctgcctggcc atctgcggcg cgagctggcg
     2161 ggccaggaag tcggagccgc cggcggcggg gtaggccacg atccagcgca gcggatggtt
     2221 cggatagagg tcggcggcac gggcggccgt gctcacgacg gcggcggacg gggccagggc
     2281 gcccgcggcc agcgcgagca gggatcggcg ttgcatcgtc atcttggagg aaggcggggc
     2341 ggtgtgacgg agtgtggaat cgaggggttg tccagcatgc ggggcgcagg cgggcgaagg
     2401 tcgtgggacg ggtcatgcgg tctcccggaa gggtggtcgt catcgaaaac cgaagcccgc
     2461 gcctcacgcg gcgggcgcca gcgcggcgac ggcgcgcgcc acgcgcgcgt cgagctcggg
     2521 cagcggcgtg cgcaggcgcg cagcgggcgc gggcgcgacc tcgcgcacgg gcgcggccca
     2581 cacgggcgcg gggaaacggc tgtcccagtc gaagcgcgcg atcacgtgcc agtgcaggtg
     2641 cggcacgacg ttgccgaggc tggccaggtt gatcttggtg ggcgcgagcg cgtcgcgcag
     2701 cacgcgttcg acggtgccca cggcgcccat catcgcggcc tgcccggcca tcgtcaggtc
     2761 gctccactcg gccacgtgcg cgcgccagac gacgcggtag aacgccggga aggccgggtc
     2821 ctcggcacgg atcacgcgcc actgcgccga ctggaacacg agcacgccgc cggcctcgcg
     2881 gcacagctcg caggacggct ggaacgacgg cgaggacagc ggatggaagt cgggcatcgg
     2941 gcggtggacg agggagcggg actgcgggcc gctccggtgg cgagccggcc gcgcgatgcg
     3001 gtattgtgtg gcag
//
LOCUS       DATCRO010000733         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_312175, whole genome shotgun sequence.
ACCESSION   DATCRO010000733 DATCRO010000000
VERSION     DATCRO010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312175"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VIN75_19830"
     CDS             complement(<1..701)
                     /locus_tag="VIN75_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8976476.1"
                     /translation="MRASRLLSLLMLLQSRGRMSAQALAEALEVSVRTIHRDVDELSA
                     AGVPIWADRGRLGGFQLQAGWRTKVDGLTAPEAQAMFLGGLPGPASELGLGEAMASAQ
                     LKLMAALPDGWREDARRVSARFHLDPIDWYRGPSATDHLPTIAQAVWSERRIAMRYES
                     WKGEVSRRVDPLGLVLKAGIWYLAAQVAKSDKAGVRTYRLSNILDLEVTDEGFERPAD
                     FDLGAWWLASTRRFE"
     gene            791..1549
                     /locus_tag="VIN75_19835"
     CDS             791..1549
                     /locus_tag="VIN75_19835"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY8976477.1"
                     /translation="MSTIEVAEALSVLELRQYTLHPGRRDELITLFEREFIEPQEDLG
                     IRIVGTFRDLDDPDRFVWLRGFADMEARPGALHAFYYGPVWQAHREKANATMIDSDNV
                     LLLRPLGCDQGLRAALQPRPAAGAAARAGGVFTITVCPLRRPADDALVHAFDQHVHPW
                     WVGVGGDLLACWVSETAANDFPRLPVRENEPVIAWITRFDDEAAERRHATLLQASGCL
                     ARPGWWQHLSGPPTQLRLVPTDRSALRRRVSEEG"
     gene            1554..2054
                     /locus_tag="VIN75_19840"
     CDS             1554..2054
                     /locus_tag="VIN75_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976478.1"
                     /translation="MTDNDYIGRPGDFDFLVGGTWLVADRRLRERHAGCTEWIHFEHT
                     CNAITMMDGRISSDENVFASAGFTGITFRTLDTTTHRWAIYWISSRDGKLQPPVHGGW
                     NGDRGVFQGDDLDEGRPVRVRFLWERLGPDQARWSQDFALVGQDGAPDGDWETNWVME
                     LRRVRG"
     gene            complement(2044..3228)
                     /locus_tag="VIN75_19845"
     CDS             complement(2044..3228)
                     /locus_tag="VIN75_19845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976479.1"
                     /translation="MTSSPSRNPGLDTLRASAIALVFMYHYAIFVSGAATFGWLGDIG
                     WTGVDLFFVLSGYLIANQLFAGIARGHALSLPRFYARRAFRTLPVFWLVLAAFALFPS
                     ALGGRTPPPWWRFLTFTQNIGLQPGTAFSHAWSLCVEEQFYLVLPATLAAGAWLARGR
                     VTLTRAHGWALMAALVAVGVGARCVLWQKYGQPADGRGDGYMAWVYYSTLCRFDEFIP
                     GVAVAMLRNFHRPAWESLVARGGLLSAIGAAAVAVMMTLAGRFYYDGTGWGFFMTAFG
                     YSLIACAFAILVMAALSPSARVLRWRIPGAQSLALWSYSTYLSHKPLAYFLARQLKPL
                     GVSDGARLAIITVACVAAGSLLYKLVEAPFMALRDRLVPSNFDAAARPAALPAAVDAI
                     SP"
BASE COUNT          520 a         1208 c         1160 g          416 t
ORIGIN      
        1 ttctcgaagc gcctcgtcga ggcgagccac cacgcaccga ggtcgaagtc cgccgggcgc
       61 tcgaagccct cgtcggtgac ctccagatcg aggatgttcg acagccggta ggtgcgcacg
      121 cccgccttgt ccgacttcgc gacctgcgcg gccaggtacc agatgcccgc cttgagcacc
      181 agccccagcg gatcgacgcg gcgcgacact tcgcccttcc agctctcgta gcgcatcgcg
      241 atgcggcgct cgctccagac cgcctgcgcg atcgtcggca ggtggtcggt ggccgacggc
      301 ccgcgatacc agtcgatcgg gtcgaggtgg aagcgtgcgc tcacgcggcg cgcgtcctcg
      361 cgccagccgt ccggcagcgc ggccatcagc ttgagctgcg ccgaggccat cgcctcgccc
      421 aggcccagct ccgacgcggg ccccggcagg ccgcccagga acatcgcctg cgcctcgggc
      481 gcggtcaggc cgtcgacctt ggtgcgccac cccgcctgca gctggaagcc gcccaggcgg
      541 ccccggtcgg cccagatcgg cacgcccgcg gcgctcagct cgtcgacgtc gcggtggatg
      601 gtgcgcaccg acacctccag cgcctcggcc agcgcctgcg cgctcatccg gccgcggctt
      661 tgcagcagca tcaacagcga caggaggcgg ctggcacgca tggacgcagt ctgcctcaaa
      721 aacatgacat gcggtggcat gcattggctg cgagcatgcg gccatcgctt ttcatcgagg
      781 agtccccgcc atgtccacca tcgaagtcgc cgaggcgctg tcggtgctcg agctgcgcca
      841 gtacacgctg catcccggcc gccgcgacga gctcatcacg ctgttcgagc gcgaattcat
      901 cgagccgcag gaggatctcg ggatccgcat cgtcggcacc tttcgcgacc tcgacgaccc
      961 cgaccgcttc gtctggctgc gcggctttgc cgacatggag gcgcgccccg gcgcgttgca
     1021 cgcgttctac tacgggccgg tctggcaagc acaccgcgag aaggccaacg ccacgatgat
     1081 cgacagcgac aacgtgctgc tgctgcgccc gctcggctgc gaccagggcc tgcgggccgc
     1141 gctgcagccg cgtccggcgg ccggcgcggc ggcgcgcgcg ggcggcgtct tcacgatcac
     1201 cgtctgcccg ctgcgccggc cggccgacga cgcgctcgtg catgcgttcg accagcacgt
     1261 gcacccgtgg tgggtgggcg tgggcggcga cctgcttgcc tgctgggtca gcgagaccgc
     1321 cgccaacgac ttcccgcgcc tgcccgtgcg cgagaacgag cccgtgatcg cctggatcac
     1381 ccgcttcgac gacgaggccg ccgagcgccg ccacgccacg ctgctgcagg cctcgggctg
     1441 cctcgcgcgg cccgggtggt ggcagcacct gtccggccca ccgacgcagc tgcggctcgt
     1501 ccccaccgac cgatcggcgc tgcgccgccg tgtttccgag gagggctgac accatgaccg
     1561 acaacgacta catcggccgc cccggcgact tcgacttcct ggtcgggggc acctggctcg
     1621 tcgccgaccg ccgcctgcgc gagcgccacg ccggctgcac ggagtggatc cacttcgagc
     1681 acacctgcaa cgcgatcacg atgatggacg ggcgcatctc gtccgacgag aacgtcttcg
     1741 ccagcgcggg cttcaccggc atcaccttcc gcacgctgga cacgaccacc catcgatggg
     1801 cgatctactg gatcagcagc cgcgacggca agctgcagcc gccggtgcac ggcggctgga
     1861 acggcgaccg cggcgtgttc cagggcgacg acctcgacga gggccggccg gtgcgcgtgc
     1921 gattcctgtg ggagcggctg gggcccgacc aggcgcgctg gtcgcaggac ttcgccctgg
     1981 tcggccagga cggtgcgccc gacggcgact gggagacgaa ctgggtgatg gagctgcggc
     2041 gcgtcagggg ctgatcgcgt cgacggcggc cggcagcgcc gccggccgcg cggcggcgtc
     2101 gaagttcgac ggcaccagcc ggtcgcgcag cgccatgaac ggcgcctcca ccagcttgta
     2161 cagcaggctg cccgcggcga cgcaggccac ggtgatgatc gccaggcgcg cgccgtccga
     2221 tacccccaag ggcttgagct gccgggcgag gaagtacgcg agcggcttgt gcgacaggta
     2281 ggtggagtac gaccacagcg ccagcgactg cgcgcccgga atgcgccagc gcagcacgcg
     2341 cgccgacggg ctgagcgcgg ccatgacaag gatggcgaag gcgcaggcga tcagcgagta
     2401 gccgaaggcg gtcatgaaga agccccagcc ggtgccgtcg tagtagaacc tgccggccag
     2461 cgtcatcatc acggcgaccg ccgcggcgcc gatcgcgctc agcagaccgc cgcgcgccac
     2521 gaggctctcc caggccgggc gatggaagtt cctgagcatc gccaccgcca ccccgggaat
     2581 gaactcgtcg aagcggcaca gcgtgctgta gtagacccag gccatgtagc catcgccgcg
     2641 gccgtcggcc ggctgaccgt acttctgcca gaggacgcag cgcgcgccga cgcccaccgc
     2701 caccagcgca gccatcagcg cccacccgtg cgcgcgcgtg agcgtcacgc gtccgcgcgc
     2761 gagccacgcc cccgcggcca gggtggccgg cagcaccagg tagaactgct cctcgacgca
     2821 caacgaccac gcgtgcgaga acgccgtccc gggctgcagg ccgatgttct gcgtgaacgt
     2881 gaggaagcgc caccacggcg gcggcgtgcg cccgcccagc gccgacggga acagcgcgaa
     2941 cgccgcgagc acgagccaga acaccggcag cgtgcggaac gcgcggcgcg cgtagaagcg
     3001 cggcagcgac agcgcgtgcc cgcgcgcgat gcccgcaaac agctggttgg cgatcaggta
     3061 gccgctcagc acgaagaaca ggtcgacgcc ggtccagccg atgtcgccga gccagccgaa
     3121 ggtggccgcg ccgctcacga agatggcgta gtggtacatg aacaccagcg cgatggcgct
     3181 ggcgcgcagg gtgtcgaggc cggggttgcg cgaaggcgat gaggtcatgg cggggtcgga
     3241 gcggccgttc tgcaaagggt tccgcggggt cgcgaacgcg gatcgggacg acccgcccta
     3301 agag
//
LOCUS       DATCRO010000734         8650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_952817, whole genome shotgun sequence.
ACCESSION   DATCRO010000734 DATCRO010000000
VERSION     DATCRO010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952817"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VIN75_19850"
     CDS             <1..557
                     /locus_tag="VIN75_19850"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8976480.1"
                     /translation="AVHDAFTGLLARKVDRRRVIALAEYPRPGTDDQTDFYIAERKPG
                     AVLRSYMMGDAPAITVNAGTTEEWVIENWTNELHTFHIHQLHFRVLEVDGQAVAAPPL
                     LDVVEVPYATATGLHSKEGPVRPGRIRIRMTFPAALAGDIPFHCHLVDHEDNGMMAVL
                     RVEKPGAPPPIAATAPMAAMHHGM"
     gene            620..1642
                     /locus_tag="VIN75_19855"
     CDS             620..1642
                     /locus_tag="VIN75_19855"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY8976481.1"
                     /translation="MFRRPPRALVALVIALSASAASPWGADGHRTVGALADRLLAGTH
                     AQAQVRALLDGLTLEQAAVWADCAKGVDPAKGYAYTSAGKFPECAIFETPQGEADMID
                     YVRRNDTNCPRAPGDSPCHAQYHYTDESFQRRRYHLGDVGTREVDVVAAISATIRVLQ
                     GQPAPAPFDIKDKREALLLLAHFVGDIAQPLHVGTPYLQADGSVVDTESGTFDPATDT
                     QGGNRIVTIRVATNHRSETLHATWDDIPEALHSNHIDAAWLALARAEPATRGDLQGWS
                     AQWASSTLTQARLALRDLRFGPEQGAQWTVPLTGRYDDSMAPIKKRQLTAAGARLART
                     LMAIWP"
     gene            complement(1639..2712)
                     /gene="pstS"
                     /locus_tag="VIN75_19860"
     CDS             complement(1639..2712)
                     /gene="pstS"
                     /locus_tag="VIN75_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY8976482.1"
                     /translation="MTNLRLSLTLALAGAALANAAPAAPVDPITGAGSSAAAPVYKIW
                     GSEYAKARGATLDYAPVGSGAGMAKINRHEVDFGASDVIASAADLKKNDLVMFPTVIT
                     GVVPVVNLGKIGPNQLRLTGDVLGRIFTGQVTQWDAPEIKALNPGLKLPSHAIRVVAR
                     ADGSGTTYHFSDYLSRTSPAWKAKYGVASHFDWPAGTLAVKGSGEVARTVQATEDSIG
                     YIDYNYVLDDDLAGVSLKNADGQWVAASVDGFHQAVMHSTWYTSGDFSTELNDLGGPN
                     TWPITMGTYVAVPRVAAAGDRAERTLRFLTWAYLHGDALARQAKFVPLPERVQASAYR
                     EISRVMGVHGESLGAKTMGQLVD"
     gene            2990..3580
                     /locus_tag="VIN75_19865"
     CDS             2990..3580
                     /locus_tag="VIN75_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976483.1"
                     /translation="MKKPLLAAAFAAALIAPAAHATTVAIAGDGSWNEFNVDSFLAQD
                     FGKGWIDTNDGSALDFAFTIGAGQHGLLTVVDAGFAGDTFTITSGGNVLGTTSAVPAG
                     GTNGALVFDFDAALANPAYSHGTFTLGAGTYSISGSLLASVAGVDGAPLDATNGALRL
                     SVSAVPESTDAALMLAGMAALGLLARRRKADSADAA"
     gene            3596..5515
                     /locus_tag="VIN75_19870"
     CDS             3596..5515
                     /locus_tag="VIN75_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEY8976484.1"
                     /translation="MKTTLLALVAALGTAFAGAAAAQTTTAPINVKIIAFNDFHGNLQ
                     SPGTFGQNTVVPSAQRPAVGGAEYIAAYVARLKAQNPNNAVVGGGDFIGASPLISALF
                     FDEPAVEVMNHVGVDFTSVGNHEFDKGSAELKRLQNGGCRITNGAQDPNSCKGLGSAA
                     PGTFDGAHFKWLSANVIENATGRTLLPPYGIKTFNGVKVAFIGMTLKGTPGIVTPTGV
                     AGLTFQDEADTVNALVPRLRAQGIEAIVVLVHQGGFQTSPNVGDINGCDGNLKNADGS
                     KSDIENIVNRLDDAVDLVISAHTHAAYNCSAGTVDVKNVGGVARSTPRPTGLANKTGR
                     LIPVTSASAFGRIVTDIDLQLDPRTHDVISVKPTNRLVDRTDPAVNAAITADPTVRNI
                     VSGYNALVSPLSSAVVATIAGALPNSANGAGEMPAGDLIADAQLAATQPASLGGAQIA
                     FMNAGGVRNPGFVTATNTYPYALTYGDAFTVQPFGNSLVTMTVTAAQLKALLEQQFSG
                     CNGQTANRVMQSSNGLKYSWSASAPACAKIVDASFTPTDVTVAPPVATGPTEIIVSGG
                     VMQNPAKTYRITVNNFMATGGDGFGVLLGGSNVKGGAQDIDALVAYLANFKSPRPAYD
                     PSLPAMGIPRVTLLP"
     gene            5512..6651
                     /locus_tag="VIN75_19875"
     CDS             5512..6651
                     /locus_tag="VIN75_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976485.1"
                     /translation="MSVVARARRASVALLILGACLSLPAHEDEAAAAARMPQRAALLA
                     RAQAELERGDAGPALDDFERAAMMLHAADAELGLIRAAMQDGQYRRALAFCAHTAGEH
                     TDDAQGGALYAWLLRVGGQPAQASRTLADVRTHAPEDPLAAAVDRALASSQPPIATGT
                     LLQPPQRMAPWQVMLEAQPAPPASASFASNAVLVDDGAAALVPRSALPWPAPARVWVR
                     NGLGQATAATLDAGDAALSSRGLARLLLQARMPVGASTASTARAPFAGSPGYVLQFGT
                     GDAPAWPLLTQGFLGGLVRGTDLRKLGFEGSPGAAVLDARGALVGITLASVDGDVTWL
                     PLASSAPLQAPSPSPSPAGLALAAPDQAYEAGLRRALQVLVDAPR"
     gene            6763..7464
                     /locus_tag="VIN75_19880"
     CDS             6763..7464
                     /locus_tag="VIN75_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8976486.1"
                     /translation="MTDKVALITAGGSGMGAAAARRLAADGYKVAVLSSSGKGEALGR
                     ELGGLGVTGSNQSEDDLRRLVDQSLERWGRIDVLVNSAGHGPRGPVLDISDADWHRGM
                     DTYLLNVIRPTRLVVPAMKRQRAGAIINISSAWAVEPAAMFPTSAVFRAGLHAFTKIF
                     ADTHAADNIRMNNVLPGWIDSLPATEERRQSVPMRRYGSSEEIAATIAFLASEGGAYI
                     TGQNLRVDGGLTRSL"
     gene            complement(7535..7610)
                     /locus_tag="VIN75_19885"
     tRNA            complement(7535..7610)
                     /locus_tag="VIN75_19885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7575..7577),aa:Gly,seq:gcc)
     gene            complement(7619..7692)
                     /locus_tag="VIN75_19890"
     tRNA            complement(7619..7692)
                     /locus_tag="VIN75_19890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7658..7660),aa:Cys,seq:gca)
     gene            complement(7776..7851)
                     /locus_tag="VIN75_19895"
     tRNA            complement(7776..7851)
                     /locus_tag="VIN75_19895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7816..7818),aa:Gly,seq:gcc)
     gene            complement(8039..>8650)
                     /locus_tag="VIN75_19900"
     CDS             complement(8039..>8650)
                     /locus_tag="VIN75_19900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8976487.1"
                     /translation="VGGDLLTIESAVMPGKGNIIRTGSLGDVMKESVEAARTVIRSRA
                     ARLGIKDELFEKRDIHIHVPDGATPKDGPSAGAAMATAFVSALTGIPVRANVAMTGEI
                     TLRGEVTAIGGLKEKLLAAHRGGITTVMIPEENVKDLQDIPENVKSQIEIIPVRWVDK
                     VLEVALESQPQALPDEEVAKEAAAAPVVEAPVKPADGVESIKH"
BASE COUNT         1296 a         3162 c         2948 g         1244 t
ORIGIN      
        1 ccgccgtgca cgacgccttc acaggcctgc tggcgcgcaa ggtcgaccgc cgccgcgtca
       61 tcgcgctggc cgagtacccg cgcccgggca ccgacgacca gaccgatttc tacatcgccg
      121 agcgcaagcc gggtgccgtg ctgcggtcct acatgatggg cgacgcgccc gcgatcaccg
      181 tcaacgccgg cacgaccgag gagtgggtga tcgagaactg gaccaacgag ctgcacacct
      241 tccacatcca ccagttgcac ttccgcgtgc tggaggtcga cgggcaggcc gtcgccgcgc
      301 cgccgctgct cgacgtcgtc gaggtgccct acgccacggc caccggcctg cacagcaagg
      361 aaggcccggt gcgcccgggg cgcatccgga tccgcatgac gttccccgcg gcattggccg
      421 gggatatccc cttccattgc cacttggtcg accacgagga caacggcatg atggcggtgc
      481 tgagagtcga gaagcccggt gcgccgccgc cgatcgcggc cacggcgccc atggccgcga
      541 tgcaccacgg catgtgaccg tcgcaccgct gacacgggcg gcgcgcacgc tcgccgcatc
      601 tcgttgtcga gggtctgcca tgttccgtcg tccacctcgc gcgctggtcg cgctcgtcat
      661 cgccctgtcc gccagcgcgg cgtccccctg gggcgcggac ggccatcgca cggtcggcgc
      721 cctggccgac cggctcctcg cgggcaccca cgcgcaggcg caggtgcgcg cgctgctgga
      781 cggcctcacg ctggagcagg ccgccgtgtg ggccgactgc gccaagggcg tcgatccggc
      841 caagggctac gcgtacacgt cggcgggcaa gttccccgag tgcgcgatct tcgagacgcc
      901 ccagggcgag gcggacatga tcgactacgt ccggcgcaac gacacgaact gcccgcgcgc
      961 gccgggcgac tcgccgtgcc acgcccagta ccactacacc gacgagtcct tccagcgccg
     1021 ccgctatcac ctgggcgacg tgggcacgcg cgaggtcgac gtcgtcgccg ccatctccgc
     1081 cacgatccgc gtgctccagg gccagcccgc gccggcgccg ttcgacatca aggacaagcg
     1141 cgaggcgctg ctgctgctgg cgcacttcgt cggcgacatc gcgcaaccgc tgcacgtggg
     1201 caccccctac ctgcaggcgg acggcagcgt cgtggacacg gagtcgggca ccttcgatcc
     1261 ggcgacggac acgcagggcg gcaaccgcat cgtcaccatc cgcgtggcca ccaaccaccg
     1321 cagcgagacg ctgcacgcca cctgggacga cattcccgag gcgctgcatt cgaaccacat
     1381 cgatgccgcg tggctggcgc tggcgcgcgc cgagccggcc acgcgcggcg acctgcaggg
     1441 ctggtcggcg caatgggcga gcagcacgct gacgcaggcc cgtctcgcgc tgcgggacct
     1501 gcgcttcggc cccgagcagg gcgcgcagtg gacggtgccg ctgacgggcc gctacgacga
     1561 ttcgatggcg ccgatcaaga agcgccagct cacggccgcg ggggcgcgcc tggcgcgcac
     1621 gctgatggcg atctggcctt agtcgaccag ctggcccatc gtcttggcgc ccagcgattc
     1681 gccgtggacg cccatcacgc gcgagatctc gcggtacgcg ctcgcctgca cgcgctcggg
     1741 cagcggcacg aacttggcct ggcgtgccag cgcgtcgccg tgcaggtagg cccaggtcag
     1801 gaagcgcagc gtgcgctcgg cgcggtcgcc ggccgccgcg acgcgcggca ccgccacgta
     1861 cgtgcccatc gtgatgggcc aggtgttggg gccgccgagg tcgttgagct cggtggagaa
     1921 gtcgcccgag gtgtaccagg tgctgtgcat caccgcctgg tggaagccgt cgacgctggc
     1981 ggccacccac tggccgtcgg cgttcttcag ggacacgccg gcgaggtcgt cgtccagcac
     2041 gtagttgtag tcgatgtagc cgatcgagtc ctcggtcgcc tgcaccgtcc tggccacctc
     2101 gccgctgccc ttgacggcca gcgtgcccgc cggccagtcg aagtggctcg caacgccata
     2161 cttcgccttc caggccgggc tcgtgcgcga gaggtagtcc gagaagtggt acgtggtgcc
     2221 cgagccgtcg gcgcgcgcga cgacgcggat cgcgtggctc ggcagcttca ggccggggtt
     2281 gagcgccttg atctcgggcg cgtcccattg cgtgacctgg cccgtgaaga tgcggcccag
     2341 cacatcgccg gtcaggcgca gctggttcgg gccgatcttg ccgaggttca cgacgggcac
     2401 gacgccggtg atgaccgtcg ggaacatcac gaggtcgttc ttcttcaggt cggccgcgct
     2461 cgcgatgacg tcggacgcgc cgaagtcgac ctcgtgccgg ttgatcttgg ccatgccggc
     2521 gcccgagccc accggggcgt agtcgagcgt ggcgccgcgc gccttggcgt actcgctgcc
     2581 ccagatcttg tagacgggtg cggcggccga ggagccggcg ccggtgatgg ggtcgacggg
     2641 ggccgcaggc gcggcgttcg cgagggcagc gccggcaagg gccagggtga gggaaaggcg
     2701 gaggttcgtc atggaaaggg agggccaacg acagtttttt gagggcaacc cggcattttc
     2761 cctgatcgcg atgacaaggt catgatcatc ccgtcacggc agccctggac cggcaccttc
     2821 atgcgacggt cgcgccggcc gtcgaacgtg ggagccggcg cctgcacgcc gccgcgaccg
     2881 gacccgccga catggatcgg tcacggcgcg gtcacggcga tctccgacag tcgcagctcc
     2941 cgtgcgtccc acgaggtcgc gcgccatgaa cgacaacgga gtcagaccca tgaaaaagcc
     3001 cctgttggcc gcggccttcg ccgccgccct gatcgcgccc gcggcgcacg cgaccaccgt
     3061 cgccatcgcc ggcgacggca gctggaacga attcaatgtc gacagcttcc tcgcgcagga
     3121 cttcggcaag ggctggatcg acacgaacga cggttccgcc ctcgacttcg ccttcaccat
     3181 cggcgcgggc cagcacggcc tgctgacggt cgtcgacgcg ggcttcgcgg gcgacacctt
     3241 caccatcacc agcggaggca acgtcctcgg caccacgtcc gcggtgccgg cgggcggcac
     3301 caacggcgca ctcgtcttcg atttcgacgc cgcgcttgcg aacccggcat acagccacgg
     3361 cacgttcacg ctcggcgccg gcacctacag catcagcggc tcgctgctgg cgtcggtggc
     3421 gggcgtcgac ggcgccccgc tggacgccac caacggcgcg ctgcgcctga gcgtgtcggc
     3481 cgtccccgaa tcgacggacg ccgcgctgat gctggccggc atggccgccc tcggcctgct
     3541 ggcgcgccgc cgcaaggccg actccgccga cgcggcctga aggagggacg ccatcatgaa
     3601 gaccaccctg ctcgccctcg tcgccgcact cggcaccgcc ttcgccggcg ctgccgccgc
     3661 gcagaccacg accgcgccga tcaacgtcaa gatcatcgcc ttcaacgatt tccacggcaa
     3721 cctgcagtcg cccggcacct tcggccagaa caccgtcgtg ccgagcgcgc agcgcccggc
     3781 cgtcggcggc gccgaataca tcgcggcgta tgtcgcgcgg ctgaaggcgc agaacccgaa
     3841 caacgcggtc gtcggtggcg gcgatttcat cggcgcctcg ccgctgatct ccgccctgtt
     3901 cttcgacgag cctgcggtcg aggtgatgaa ccacgtcggc gtcgacttca cctccgtcgg
     3961 caaccacgag ttcgacaagg gttcggccga gctcaagcgc ctgcagaacg gcggctgcag
     4021 gatcaccaac ggcgcgcagg atccgaacag ctgcaagggc ctgggctccg cggcacccgg
     4081 caccttcgac ggcgcgcact tcaagtggct gtcggccaac gtgatcgaga acgccaccgg
     4141 ccgcacgctg ctgccgccgt acggcatcaa gacgttcaat ggcgtcaagg tggccttcat
     4201 cggcatgacg ctcaagggca cgccgggcat cgtcacgccc accggcgtcg cgggcctgac
     4261 gttccaggac gaggccgaca ccgtcaacgc gctcgtgccg cgcctgcgcg cgcagggcat
     4321 cgaggcgatc gtcgtgctgg tgcaccaggg cggcttccag accagcccca acgtcggcga
     4381 catcaacggt tgcgacggca acctcaagaa cgcggacggc agcaagtccg acatcgagaa
     4441 catcgtcaac cgcctcgacg atgcggtcga cctcgtgatc agcgcgcaca cgcacgccgc
     4501 gtacaactgc tcggccggca cggtcgacgt gaagaacgtc ggcggcgtcg cgcggtccac
     4561 cccgcgcccc acggggctcg cgaacaagac gggccgcctg atcccggtga ccagcgcgag
     4621 cgccttcggc cgcatcgtca ccgacatcga cctgcagctc gatccgagga cgcacgacgt
     4681 catctccgtc aagccgacca accgcctggt cgaccgcacc gaccccgcgg tcaacgccgc
     4741 gatcacggcc gacccgacgg tgcgcaacat cgtgagcggc tacaacgcgc tcgtctcgcc
     4801 gctgtccagc gcggtcgtcg cgacgatcgc cggcgcgctg ccgaactcgg ccaacggcgc
     4861 cggcgagatg cccgcgggcg acctgatcgc cgacgcacaa ctggccgcca cgcagccggc
     4921 ctccctcggc ggcgcgcaga tcgcgttcat gaatgccggc ggcgtgcgca accccggctt
     4981 cgtcacggcg acgaacacgt acccgtacgc gctcacgtac ggcgacgcgt tcaccgtgca
     5041 gccgttcggc aacagcctgg tcacgatgac cgtcaccgcc gcgcagctga aggcgctgct
     5101 cgagcagcag ttcagcggct gcaacgggca gacggccaac cgcgtgatgc agtcgtccaa
     5161 cggcctgaag tactcgtgga gcgccagcgc gccggcgtgc gcgaagatcg tcgatgccag
     5221 cttcacgccg accgacgtca ccgtcgcgcc gccggtggcg accgggccga ccgagatcat
     5281 cgtcagcggc ggcgtcatgc agaacccggc caagacctat cgcatcaccg tcaacaactt
     5341 catggcgacg ggcggcgacg gcttcggggt gctgctgggc ggcagcaacg tcaagggcgg
     5401 cgcgcaggac atcgacgcgc tcgtggcgta cctcgccaac ttcaagtcgc cccggccggc
     5461 ctatgacccg agcctgccgg cgatgggcat cccgcgcgtg acgctgctgc cttgagcgtc
     5521 gtcgcacgag cccggcgcgc gagcgtcgcg ctgctgatcc tcggggcatg tctttccctg
     5581 cctgcccacg aggacgaggc cgccgccgcc gcgcgcatgc cacagcgcgc ggcgctgctg
     5641 gcgcgcgcgc aggccgagct cgaacgcggc gacgccggcc ccgcactcga cgacttcgaa
     5701 cgcgcggcga tgatgctgca cgccgccgac gccgagctgg ggttgatccg cgccgcgatg
     5761 caggatggcc agtaccgccg cgcgctcgcc ttctgtgccc acacggccgg cgagcacacg
     5821 gacgatgccc agggcggcgc gctgtacgca tggctgctgc gcgtcggcgg ccagccggcg
     5881 caggcctcgc gcacgctcgc ggacgtgcgc acgcacgcgc ccgaggaccc gctcgccgcc
     5941 gcggtcgatc gtgcgctggc gagctcgcag ccgccaatcg cgacgggcac gctgctgcag
     6001 ccgccccagc gcatggcgcc gtggcaggtg atgctggagg cgcagccggc gccgcccgcg
     6061 tcggcaagct tcgcatcgaa cgccgtcctg gtcgacgatg gcgcggcggc gctcgtgccg
     6121 cggtccgcgc tgccctggcc cgcgcccgcg cgcgtgtggg tgcgcaacgg cctcgggcag
     6181 gcgacggcgg cgacgctgga cgccggcgac gccgcgctgt cctcgcgcgg tctcgcgcgc
     6241 ctgctgctgc aggcccggat gcccgtcggg gcgtcgacgg catcgaccgc acgcgcgccc
     6301 ttcgccggca gccccggcta cgtcctgcag ttcgggaccg gcgacgcgcc ggcctggccg
     6361 ctgctcacgc agggcttcct cggcggcctg gtgcgcggca ccgacctgcg caagctgggc
     6421 ttcgagggca gccccggtgc ggcagtgctc gatgcaaggg gggcgctggt cggcatcacg
     6481 ctggcgtcgg tcgacggcga cgtcacctgg ctgccgttgg cgtcctcggc gcctttgcag
     6541 gcgccctcgc cctcgccctc gcccgcgggc ctcgccctgg ccgcgccgga ccaggcctac
     6601 gaggcgggcc tgcgccgcgc gctgcaggtc ctcgtcgacg cgcctcgctg aggcctcgcc
     6661 cgcgatgcct gcgccggcgt ccccggtgcg tcgccggtga taggctggcg ccgcgtcggt
     6721 gcgggccatc ggcgatctcc cgcaacgaac caggatcacg acatgacaga caaggtagcc
     6781 ctcatcaccg ccggcggcag cggcatgggc gcggcggcgg cgcgccggct cgcggcggac
     6841 gggtacaagg tggcggtgct ctcgtcatcg ggcaagggcg aggcgctggg gcgcgaattg
     6901 ggcggcctgg gcgtgaccgg ctcgaaccag tccgaggacg acctgcgccg gctcgtcgac
     6961 cagtcgctgg agcgctgggg ccgcatcgac gtgctggtca acagcgccgg ccacggcccg
     7021 cgcggcccgg tgctggacat cagcgacgcc gactggcatc gcggcatgga cacctacctg
     7081 ctgaacgtga tccgccccac gcgcctcgtg gtgcccgcga tgaagcgcca gcgcgccggc
     7141 gcgatcatca acatctcgtc ggcctgggcg gtcgaacccg cggcgatgtt cccgacctcg
     7201 gcggtgttcc gcgcgggcct gcacgcgttc acgaagatct tcgcggacac gcacgccgcc
     7261 gacaacattc gcatgaacaa cgtgctcccg ggctggatcg acagcctccc ggccaccgag
     7321 gaacggcgcc agagcgtgcc gatgcggcgc tacggcagca gcgaggagat cgcggcgacg
     7381 atcgccttcc tggcgtccga gggcggcgcc tacatcaccg gccagaacct gcgcgtcgac
     7441 ggcgggctca cgcgatcgct ctgaacgttc gccaagcggc accactatgc gccgcacaag
     7501 gcaaaacgcc tcgcggttgc gaggcgtctg actttggagc gggaaacgag gctcgaactc
     7561 gcgacctcaa ccttggcaag gttgcgctct accaactgag ctattcccgc aaattttctg
     7621 gaggcgcgat ccagagtcga actggactag acggatttgc aatccgttgc ataaccgctt
     7681 tgctatcgcg ccgaggtact gcacctcgaa ccgacggtgc cagccgtcga ttctgacaaa
     7741 aaagggaagc atgagcttcc ctgtttcgtg aatcctggag cgggaaacga ggctcgaact
     7801 cgcgacctca accttggcaa ggttgcgctc taccaactga gctattcccg caggtcactg
     7861 caatcggact acttcgctga cgtttttgcg tcgttgtttt gtcgtcttgc tgaagctggc
     7921 agtatatgcc ggatttgttt gtcttggcaa gctcgactga atcagtctgg tttgcgcgac
     7981 accggaagaa ctgcgcccgg gcgccttgcg acgaccgggc gcggattcta acccgcggtc
     8041 agtgcttgat cgactcgacg ccgtcggccg gcttgaccgg cgcctcgacc accggcgcgg
     8101 cggcggcctc cttggccacc tcctcgtcgg gcagtgcctg cggctgcgat tcgagcgcca
     8161 cctcgagcac cttgtcgacc cagcgcaccg ggatgatctc gatctgcgac ttcacgttct
     8221 ccgggatgtc ctgcaggtcc ttgacgttct cctcggggat catcaccgtg gtgatgccgc
     8281 cgcggtgcgc cgccaggagc ttttccttca ggccgccgat ggccgtgacc tcgccgcgca
     8341 gcgtgatctc gccggtcatc gccacgttgg cgcgcaccgg gatgcccgtc agggccgaga
     8401 cgaaggccgt ggccatcgcc gcgccggcgc tcgggccgtc cttgggcgtc gcgccgtcgg
     8461 gcacgtggat gtggatgtcg cgcttctcga acagctcgtc cttgatgccc agccgcgcgg
     8521 cgcggctgcg gatcacggtg cgggcggcct cgaccgactc cttcatcacg tcgcccagcg
     8581 aaccggtgcg gatgatgttg cccttgcccg gcatgacggc cgattcgatg gtgagcaggt
     8641 cgccaccgac
//
LOCUS       DATCRO010000735         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_964624, whole genome shotgun sequence.
ACCESSION   DATCRO010000735 DATCRO010000000
VERSION     DATCRO010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964624"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /gene="pyrB"
                     /locus_tag="VIN75_19905"
     CDS             <1..219
                     /gene="pyrB"
                     /locus_tag="VIN75_19905"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8976488.1"
                     /translation="SAGEYFKSFGLTEDLLAQARPDAIVMHPGPINRGVEIDSRVADG
                     SHSVILPQVTFGIAVRMAVMSILAGNDA"
     gene            216..1532
                     /locus_tag="VIN75_19910"
     CDS             216..1532
                     /locus_tag="VIN75_19910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8976489.1"
                     /translation="MKILIRNGRVVDPASARDEAADVAIAAGRIVAIGNVPADFTPDR
                     AIEADGCVVAPGLVDLAARLKEPGPGALESELAAAAAGGVTSLVCPPDMDPPLDEPGL
                     VDMLKFRARKLSRCRLFPLGALTQGLGGAEITEMIDLTEAGCVGFSQAEAPMRDNLTL
                     LRAMQYASTRGYSVWLRPKDPSLSGGVAASGAVATRLGLSGVPVASETIAIHTVVELV
                     RQTGARVHFCRLSSAAGVELVRRAKAEGLPVTADVSINSLHLTDVDIGYFNADMRLTP
                     PLRQGKDRDALRAGLADGTIDALVSDHTPIGHDDKMLPFGEAVPGATGLELLLSLALK
                     WGNESRLTLAQSLARVTSDPVRVLGEALGSLGQSAGRLVEGGVADVCVFDPAAEWQVT
                     PGALHSRGKHTPFDAASSGTRMPGRVRHTLVAGTLAYSAAAEANAA"
     gene            1529..2278
                     /locus_tag="VIN75_19915"
     CDS             1529..2278
                     /locus_tag="VIN75_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8976490.1"
                     /translation="MRTLVAIWRILRVIGMVAQGLWLIAFAMPRTDAAGWHAIMQRWA
                     VKLLRCLGVQVRVSGAPEAGAVLMVANHVSWLDIPALHASSPRTRFVSKSAIAHWPLL
                     GRLARAGGTLFIERERKRDALRVVHEVAAALKNHDAVAVFPEGTTGSGHEVLPFHANL
                     LQAAITTATQIQPIVLRYSEPGLPVSVAAQYIDDTTLVQSVMKVCRARGVVVDVRFLA
                     PEPAGELDRRALAERLHERVAAELRAVIQAG"
     gene            2480..3076
                     /locus_tag="VIN75_19920"
     CDS             2480..3076
                     /locus_tag="VIN75_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976491.1"
                     /translation="MVEFCWSWIRRHAPGVAVGWKPGRDGQRSTRPGFPATSLRGQRQ
                     EVVMNHSKSRTSYGVYSPLGCVLASFPTEGDARAAIAELEAAGFAAEDVEFQSAEEVR
                     LRATHDIHNSGLLARIGQEVNLAKSRRALAEQGHPFVAVYAPGAVATRSVAEIARHCH
                     ADFAQKFGRLIVEELIESETGGRQVPESPDTGLDFHWR"
     gene            3177..3662
                     /locus_tag="VIN75_19925"
     CDS             3177..3662
                     /locus_tag="VIN75_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976492.1"
                     /translation="MNENNSQSAYGVFKPVGHVLASFPTERDARSAVEALRQAGFPEV
                     AYYSADEVRERAERDIENAGILATIGQELNLVKQQRDLAREGHPFVSVLAEADDAARR
                     AADIVARFHADRAQKYGHMIIEELIEPGSGQRQAKESVDTGLDPQTHSGVEAGADPTR
                     H"
     gene            complement(3759..>4244)
                     /locus_tag="VIN75_19930"
     CDS             complement(3759..>4244)
                     /locus_tag="VIN75_19930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976493.1"
                     /translation="VQAPFALSHATIGAVFLLYVVGMFSSPWAGRQADRRGSPRMLAA
                     MLALCAAGLVLTLSNALALIIAGVALFTFGFFAAHSVASSWVGRLARHSKSLASAAYL
                     TTYYLGASSMGWLGGHAWQAGAWPGVLAFLGVLWLGCVVIAWRMARLAPATRPLPIRG
                     S"
BASE COUNT          613 a         1506 c         1546 g          579 t
ORIGIN      
        1 agcgcgggcg agtacttcaa gagcttcggc ctgacggaag acctgctggc gcaggccagg
       61 cccgacgcca tcgtgatgca cccgggcccg atcaaccgcg gcgtggagat cgactcgcgc
      121 gtggccgacg gctcgcacag cgtcatcctg ccgcaggtca cgttcggcat cgccgtgcgc
      181 atggccgtga tgtccattct tgccgggaac gacgcatgaa gatcctcatt cgcaacggtc
      241 gcgtcgtcga ccccgcgagc gcccgcgacg aagccgccga cgtcgcgatc gccgccggcc
      301 gcatcgtggc catcggcaac gtgcccgccg acttcacgcc cgaccgcgcg atcgaggccg
      361 acggctgcgt cgtcgcgccg ggcctggtcg acctcgccgc gcggctgaag gagcccggtc
      421 ccggcgccct ggagagcgag ctcgcggccg cggccgccgg cggcgtcacc agcctggtgt
      481 gcccgcccga catggatccg ccgctggacg agcccggcct ggtcgacatg ctcaagttcc
      541 gcgcccgcaa gttgagccgc tgccgcctgt tcccgctggg cgcgctcacg cagggcctgg
      601 gcggcgccga gatcaccgag atgatcgacc tcaccgaggc cggctgcgtc ggcttctcgc
      661 aggccgaggc gccgatgcgc gacaacctga cgctgctgcg cgcgatgcag tacgccagca
      721 cgcgcggcta ctcggtgtgg ctgcggccca aggatccgtc gctgtcgggc ggggtcgcgg
      781 ccagcggggc cgtggccacg cgcctggggc tgtcgggggt gcccgtcgcg tccgagacga
      841 tcgcgatcca caccgtcgtc gagctggtcc gccagacggg ggcccgcgtg cacttctgcc
      901 gcctgtccag cgccgcgggc gtcgagctcg tgcgccgcgc gaaggccgaa ggcctgccgg
      961 tcaccgcgga cgtcagcatc aactcgctgc acctgaccga cgtcgacatc ggctacttca
     1021 acgcggacat gcgcctgacg cccccgctgc gtcagggcaa ggaccgcgac gcgctgcgcg
     1081 cgggcctggc cgacggcacg atcgacgcgc tcgtgtcgga ccacacgccc atcggccacg
     1141 acgacaagat gctgccgttc ggcgaggccg tgcccggtgc cacgggcctg gagctgctgc
     1201 tgagcctcgc gctgaagtgg ggcaacgaga gcaggctcac gctcgcgcag tcgctcgcgc
     1261 gcgtcaccag cgatccggtg cgcgtgctgg gcgaggcgct cggctcgctg ggccagagcg
     1321 ccggccgcct ggtcgagggc ggcgtggccg acgtgtgcgt gttcgatccc gccgccgaat
     1381 ggcaggtgac gcccggggcg ctgcacagcc gcggcaagca cacgccgttc gatgccgcgt
     1441 ccagcggcac gcgcatgccg ggccgggtgc gccacacgct cgtcgcgggg acgctggcct
     1501 acagcgccgc agccgaggcg aacgccgctt gaggacgctg gtcgcgatct ggcgcatcct
     1561 gcgcgtcatc ggcatggtgg cgcaggggct ctggctcatc gccttcgcga tgccgcgcac
     1621 cgacgccgcg ggctggcacg ccatcatgca gcgctgggcc gtcaagctgc tgcgctgcct
     1681 gggcgtgcag gtgcgggtca gcggggcgcc cgaggccggc gcggtgctga tggtggccaa
     1741 ccacgtgtcg tggctggaca tcccggcgct gcacgcgagc tcgccgcgca cgcgcttcgt
     1801 gtcgaagtcg gccatcgcgc actggccgct gctgggacgt ctcgcgcgcg ccggcggcac
     1861 gctgttcatc gagcgcgagc gcaagcgcga cgcgctgcgc gtggtgcacg aggtcgccgc
     1921 tgcgctgaag aaccacgacg cggtcgccgt cttccccgag ggcactacgg gctccggcca
     1981 cgaggtgctg ccgttccacg ccaacctgct gcaggcggcc atcacgacgg cgacgcagat
     2041 ccagcccatc gtgctgcgct acagcgagcc gggcctgccc gtgagcgtcg cggcgcagta
     2101 catcgacgac acgacgctcg tccagagcgt gatgaaggtg tgccgcgcgc gtggcgtggt
     2161 ggtggacgtg cgcttcctgg cgcccgagcc cgccggcgag ctcgaccgcc gcgcgctggc
     2221 cgagcggctg cacgagcgcg tcgccgccga actgcgcgcg gtcatccagg cgggctgagg
     2281 tcgccccgca gcgggcggcg gtcgacaggg gccgggcatg gcgttgcgtt cacttggccg
     2341 gcgcgtgaca cgtcgtcggc aggacggccg gatgccgacc cggagttcgc tttttctgat
     2401 cttgcgggga tgtctcctcc cccaagaacc ggctgccgag gccgtccatc gcaagttggc
     2461 agcgtgatgg cgcgagcgta tggtcgagtt ctgctggtcg tggatccggc ggcatgctcc
     2521 aggcgtcgcc gtcgggtgga agcccggtcg tgatgggcag cggtcgaccc gtccgggatt
     2581 ccccgcgaca agcttgcgcg gtcaacgaca ggaggtcgtc atgaatcatt cgaagtcccg
     2641 aacgtcgtac ggcgtctatt cgccgctggg atgcgtgctc gcgtcgttcc cgacggaagg
     2701 cgacgcccgc gccgcgatcg ccgaactgga ggccgccggc ttcgctgccg aggatgtgga
     2761 gttccagtcg gccgaggagg tgcggctgcg cgcgacccac gacatccaca attccggcct
     2821 gctcgcgcgc atcgggcagg aggtgaatct cgcaaagtca cgccgtgcct tggcggagca
     2881 ggggcatccc ttcgtggcgg tgtacgcgcc tggcgcggtc gccacgcgca gcgttgccga
     2941 gattgcacgc cactgccatg ccgacttcgc acagaagttc ggacggctga tcgtcgagga
     3001 gctgatcgag tccgagaccg gcggccgcca ggtgcccgaa tcgcccgaca ccggcctcga
     3061 cttccactgg cggtgacgcg ccggccgccg gtgttgccgg gctgatggat gcgggcaccg
     3121 tgctgcagcg cggcggtacg tggcttgccc tttcatcgga gaaaggggca accaccatga
     3181 acgagaacaa ctcgcagtcg gcctacggcg tgttcaagcc ggtcggccac gtgctggcgt
     3241 ccttccccac ggaacgcgat gcgcgctccg ccgtggaggc gctcaggcag gccgggttcc
     3301 cggaggtggc gtactactcg gccgacgaag tgcgcgagcg cgcggaacgc gacatcgaga
     3361 acgccggtat cctggccacc atcgggcagg agctcaacct cgtcaagcag cagcgcgacc
     3421 tcgcgcgcga gggccatccg ttcgtctcgg tgctggccga ggcggacgac gcggcgcggc
     3481 gcgcggccga catcgtcgcg cgcttccatg cggaccgcgc gcagaagtac ggccacatga
     3541 tcatcgagga gctgatcgag ccgggctctg ggcagcgcca ggccaaggag tcggtcgaca
     3601 cgggtctcga cccgcagacc cattcgggcg tggaggccgg cgccgatccg acgcggcatt
     3661 gaggggtcgt gtagggcggg ttggccgcag gcgtaacccg ccttgtcggc gtgtcgcgat
     3721 gcctgcgaac ggcgggtgac gcctgcggct aacccgccct acgacccacg gatcggcaat
     3781 ggccgcgtcg cgggcgcgag gcgcgccatg cgccacgcga tcaccacgca gcccagccac
     3841 agcacgccca ggaacgcgag cacgcccggc catgcgccgg cctgccacgc gtggccgccg
     3901 agccagccca tgctgctcgc gccgaggtaa taggtggtca ggtaggccgc cgaggcgagc
     3961 gacttggaat gcctggccag ccggccgacc cagctgctgg ccaccgagtg cgcggcgaag
     4021 aacccgaacg tgaacagcgc gacaccggcg atgatcagcg ccagcgcgtt cgacagcgtc
     4081 agcacgaggc cggcggcgca cagcgcgagc atcgcggcca gcatgcgcgg cgagccgcgg
     4141 cggtcggcct gccggccggc ccacggcgac gagaacatgc ccaccacgta cagcaggaac
     4201 accgcgccga tggtggcgtg cgacagcgcg aacggtgcct gcac
//
LOCUS       DATCRO010000736        11349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_863655, whole genome shotgun sequence.
ACCESSION   DATCRO010000736 DATCRO010000000
VERSION     DATCRO010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863655"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VIN75_19935"
     CDS             complement(<1..93)
                     /locus_tag="VIN75_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family deoxyribonuclease"
                     /protein_id="HEY8976494.1"
                     /translation="MAGESAPLQWIDTHCHLDAREFDADRAAVVA"
     gene            complement(119..814)
                     /gene="lolD"
                     /locus_tag="VIN75_19940"
     CDS             complement(119..814)
                     /gene="lolD"
                     /locus_tag="VIN75_19940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY8976495.1"
                     /translation="MNETVLQATGLGRRFSEGGLDVQVLHDVDLAVRAGETLAIVGAS
                     GSGKSTLLHLLGGLDAPTTGRVMLMGQDINTMGLAQQGLFRNEHLGFVYQFHHLLPEF
                     SALDNVAMPLLIRRAKAADAHAQAKAMLDAVGLGPRAQHRPGELSGGERQRVAIARAL
                     VTRPACVLADEPTGNLDKDTANTVFALMLDLARERGTAFVLVTHDVGIAARCQRQLRL
                     DRGVLVPPRVEAV"
     gene            complement(807..2069)
                     /locus_tag="VIN75_19945"
     CDS             complement(807..2069)
                     /locus_tag="VIN75_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY8976496.1"
                     /translation="MPVPFEWLIGWRYTRAGRAGRRNAFISFISLVSMLGIGLGVAAL
                     IIVLSVMNGFQKEVTDKMLKAIAHVQLMDDTGNGLADWRALASKARALDPDVRAAAPY
                     TETQSLLAHGDGMRGALVRGIEPAEEANLTELAADLQREGILAKLTPGSWNIVLGAEL
                     ARALGVSVGDQVTVIAPGAQVTPAGALPRLKSFNVVGVFSIGHYEYDSGLALAALDDT
                     ARLYRTGGPTGVQLRLADVHQARAAANLLAQGLGGEVRVQDWTRTNAAWFDAVQIEKT
                     MMFIILTLIVAVAAFNLVSTLVMTVTDKQADIAILRTLGASPRSVMLIFMVQGAASGI
                     AGTLGGIGFGLLVAYNIPVIVPFIEHLLHTSFLPASVYLIDRMPSDPHLSEVGQIAFW
                     SLLFAFLATVYPSWRASRVRPAEALRYE"
     gene            2241..3161
                     /locus_tag="VIN75_19950"
     CDS             2241..3161
                     /locus_tag="VIN75_19950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY8976497.1"
                     /translation="MKAPDFAGASREVLSFLHQRIGMDLWLVSRADNEDWVVLAAEDH
                     GYGVKEGDVFRWADTFCSRMVRDEGPRVAPRVDEVPAYAALPFARQLPIGAYVGVPLC
                     DAQGHLFGTLCGINPRPMPSSLANEQAMVQVLGDMLSGVLSAELQASTLAREAEAARS
                     EALTDPLTGLANRRGWETALAAEEERCRRYGATACVVAIELDGLKGPAPREVCEAVLV
                     RAAQALRQTVRKPDTLARLDEDRFMVLGVECTTAEAMALLRRLEQSVQTADLSAALGM
                     AMRNRSSGLFDTFDRAVDAMVEHRDARARA"
     gene            3209..4159
                     /locus_tag="VIN75_19955"
     CDS             3209..4159
                     /locus_tag="VIN75_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976498.1"
                     /translation="MHLVIPSASALSDAAVQALGTLSLPNLERLLARWAPQEDASTAD
                     IPDADEYALSLPHERLLARLRGWPVVDGLLPFAADSARRDGLAPEPGSGWGLITPAHW
                     HVGRDQVNLVDPALLDLDEDEARTLHATLAPLFTELGWAWHWAGPTRWYVSHPSLAGL
                     PTASIDRVIGRNVDLWLNDHPGVMLVRRLQSEVQMLLYVHPLNDAREERGAMSVNSFW
                     LGGTGPAQPADKVLPADVVVDDRLRAPLLGDDWSAWAEAWHALDAGPLRALAESQAAG
                     ARLTLAGERQARSWQPAARPWWKRLVGGGGASVQSTLEAL"
     gene            4156..5925
                     /gene="recJ"
                     /locus_tag="VIN75_19960"
     CDS             4156..5925
                     /gene="recJ"
                     /locus_tag="VIN75_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8976499.1"
                     /translation="MSPQPRLHTRDVPPRTAFALEQAGVHPLLARLFAARGVRSADEL
                     DDAMGQLLPPSTLRGSAEAARLLADAIDANQRIVVVADYDCDGATACAVALRGLQMLG
                     ASSARLSYVVPDRQLHGYGLTPTIVDLACGDDGPDDARPFGAERPPKPDLLVTVDNGI
                     ASLAGVAHARARGIAVLVTDHHLPALVDDKVVLPEADVIVNPNQPECTFASKAMAGVG
                     VMFYTLLATRAELRERGRFDAATQPRLDALLDLVALGTVADVVKLDPNNRRLVAQGLK
                     RIRAGRMQPGIAALFHVAARKHERASAFDFGFALGPRLNAAGRLADMTLGIECLLTDD
                     PTRAAQLAGELDRINRERRDVEAGMREQAEALMEQIMPTGAPPAALALFDDGFHEGVV
                     GIVAGRVKDRLHRPTFVFAVGADGLLKGSGRSIAGFHLRDALDLVTKRHPGVLRKFGG
                     HSMAAGCTIAREDFETFAQAFTQVAEEWLSPSALSRVLATDGPLALEWFNTATVATLD
                     AQVWGQGFEAPTFCDEVDVVQQRLVGERHLKLRVRHAGQLRDAIWFGRSEPVPERLRI
                     AYRLSIDEFQGQQRLQMVVEAAG"
     gene            6009..8042
                     /locus_tag="VIN75_19965"
     CDS             6009..8042
                     /locus_tag="VIN75_19965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8976500.1"
                     /translation="MHRSVCRAALAVALCLPVLAGASKSSVDLGPTGNPPPRLDPLLE
                     PMLRRPTYASMVLSPDGKHIAAVGFNGAGTNSAVVMLDADTLEGNLIVQPRPWTVKGY
                     RPYIRNPRNVAWLSDSKIAVNFTVADAAIFGIDGAPGTDLMQGYVQPFRDAAGKPTSW
                     HLVVRDLARREFSRMNIEDGTNTSYDLDIDGKAVGWASDASGDIRVAQTIDTAFFSDH
                     SRIITWYRPSTSDKWVKVDERSVLDEEFHPLFVPNRPDRIVVQARNGHDMLAIWDYDV
                     AKHAFADLLAGTGTEDIAGVVLDNGLDNFNEVATDGLKRQSHWFDTRYARLQASVDAS
                     MPDHVNVIQPNPSDRVLVYSYSDRDPGRWYLLNTKAMSMKLVAQRVQGIDPARMQPMQ
                     TLHYPSFDGLSVPAYLTLPGKPAKPAPTVVLIHGGPQLRDRWQFDAEVQILAAHGYAV
                     FQPQFRGSSGFGKHFEEAGYGQWGQAMQEDIDAGVKYLVDQKIADPQRLCIVGASYGG
                     YAALWALAKDPQLFKCGASVAGVSDLRRMLHDDSDMSHNAVAREIVRHRLGDGSEMKA
                     TFDSVSPVKHADRIVAPLLLVHGDLDQRVPVSHGKEMHEALVDLKKDVVWIEFPDERH
                     GIAKPQNQRIYFDALFKLLERTIGKGEPPFPAASPAAQNQEIPLSPVDSIDTP"
     gene            complement(8058..8261)
                     /locus_tag="VIN75_19970"
     CDS             complement(8058..8261)
                     /locus_tag="VIN75_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976501.1"
                     /translation="MLPMLNPRQINLLFKTAAGEPEPATAQARRELAELRQLGLLSFD
                     EGGRFAMTAQGKARLRQYAPSAH"
     gene            8408..9193
                     /locus_tag="VIN75_19975"
     CDS             8408..9193
                     /locus_tag="VIN75_19975"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY8976502.1"
                     /translation="MTHLLPASTPRRGLAPSCLAAVAVAAGLSAQPSTAAAAAACAAR
                     SGPTVPTVVELYTSEGCSSCPPAERWLGALNGRDGVVALAFHVDYWDYLGWKDRFAQA
                     LFTQRQNASQRTSGARFAYTPQVILDSRDAPAWSTLPATALQSRALATVALALARDDG
                     GLSLTVTPGPGAPAALSGYVAVVDDGLRTQVGAGENRGATLRQDAVVRELLPWTLAGR
                     QAATLRFSPSAAPEAGAARHWVAVATDGPYGRPVQALTLACAR"
     gene            9423..10655
                     /locus_tag="VIN75_19980"
     CDS             9423..10655
                     /locus_tag="VIN75_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEY8976503.1"
                     /translation="MTQHDPLSLHVPEPTGRPGCATDFSYLHVSPAGEVRRPPVDTDP
                     RSTNDLAFSLVRVLDDDGNAVGAWAPEVSDELLRTGMKAMMKTRIFDARMLIGQRQKK
                     MSFYMQSLGEEAIGSAHALALEEGDMCFPTYRQQSLLIVRDYPLVDMICQLLSNERDP
                     LKGRQLPVMYSVKAKGFFSISGNLATQYIQAVGWAMASAIKNDTRIASAWIGDGATAE
                     SDFNTALTFAHVYNAPVILNVVNNQWAISTFQAIAGGEHTTFAARGVGSGIASLRVDG
                     NDFLAVYAASKWAAERARRNLGPTLIEWVTYRAGPHSTSDDPSKYRPADDATRFPLGD
                     PIGRLKQHLIKRGCWSEAEHEEVQKALEAEVAAAQKEAESYGTLNDGRIPSAASMFED
                     VYKEMPEHLRRQRQELGV"
     gene            10777..>11349
                     /locus_tag="VIN75_19985"
     CDS             10777..>11349
                     /locus_tag="VIN75_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8976504.1"
                     /translation="MEMQDKKTLAAQPQGPTAPMTMIQALRSAMDVMLERDPNVVIFG
                     QDVGYFGGVFRCTEGLQKKYGKQRVFDAPISEGGIVGAAVGMGAYGLRPVVEIQFADY
                     FYPASDQIVSEAARLRYRSAGDFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTHVCGLR
                     TVMPSNPYDAKGLLISCIENDDPVIFLEPKR"
BASE COUNT         1689 a         4146 c         3947 g         1567 t
ORIGIN      
        1 cgccaccacg gcggcgcgat cggcatcgaa ctcgcgggcg tcgaggtggc agtgggtgtc
       61 gatccactgc agcggggcgg actcgccggc catgcgctcc cccggcacgc gacccgcctc
      121 agacggcctc gaccctgggc ggcaccagca cgccgcggtc caggcgcagc tggcgctggc
      181 agcgcgccgc gatgcccacg tcgtgcgtca ccagcacgaa ggccgtgccg cgttcgcgcg
      241 cgaggtccag catcagcgcg aagacggtgt tggccgtgtc cttgtcgagg ttgcccgtgg
      301 gctcgtccgc gagcacgcac gcgggccgcg tgacgagcgc gcgcgcgatc gccacgcgct
      361 ggcgctcgcc gcccgagagc tcgccgggcc ggtgctgggc gcgcgggccc aggccgaccg
      421 cgtcgagcat cgccttggcc tgcgcgtgcg cgtcggccgc cttggcgcgg cggatcagca
      481 gcggcatcgc gacgttgtcg agcgccgaga actcgggcag caggtggtgg aactggtaga
      541 cgaagcccag gtgctcgttg cggaacaggc cctgctgcgc gaggcccatc gtgttgatat
      601 cctggcccat cagcatgacg cggccggtgg tgggcgcatc gaggccgccc agcaggtgca
      661 gcagcgtgct cttgcccgag cccgaggcgc cgacgatcgc cagcgtctcg ccggcccgca
      721 ccgccaggtc gacgtcgtgc agcacctgca cgtcgaggcc gccttcggag aagcggcggc
      781 ccaggccggt ggcctgcagc acggtctcat tcatagcgca gggcctccgc gggacgcacg
      841 cggctggcgc gccagctcgg gtagacggtg gcaaggaagg cgaacagcag cgaccagaag
      901 gcgatctggc cgacttcgga caggtgcggg tcgctgggca tgcggtcgat caggtacacg
      961 ctcgcgggca ggaagctggt gtgcagcagg tgctcgatga agggcacgat caccgggatg
     1021 ttgtaggcca ccagcaggcc gaagccgatg ccgccgagcg tgccggcgat gcccgaggcc
     1081 gcgccctgca ccatgaagat cagcatcacc gagcgcgggc tggcgcccag cgtgcgcagg
     1141 atcgcgatgt cggcctgctt gtcggtgacc gtcatcacca gcgtcgagac gaggttgaac
     1201 gcggccacgg ccacgatcag cgtgaggatg atgaacatca tcgtcttctc gatctgcacg
     1261 gcgtcgaacc aggcggcgtt ggtgcgcgtc cagtcctgca cgcgcacctc gccgcccagg
     1321 ccctgggcga ggaggttggc ggcggcgcgg gcctggtgca cgtcggccag gcgcagctgc
     1381 acgccggtgg ggccgccggt gcggtaaagc cgcgccgtgt cgtcgagcgc ggccagcgcc
     1441 aggccgctgt cgtactcgta gtggccgatc gagaagacgc cgaccacgtt gaacgacttc
     1501 agccgcggca gcgcgcccgc gggcgtcacc tgcgcgccgg gggcgatcac ggtcacctgg
     1561 tcgcccacgc tcacgccgag cgcgcgggcg agctcggcgc ccagcacgat gttccagctg
     1621 cccggcgtga gcttggccag gatgccctcg cgctgcaggt cggccgccag ctcggtgagg
     1681 ttcgcctcct cggccggctc gatgccgcgc acgagggcgc cgcgcatgcc gtcgccgtgc
     1741 gccagcagcg actgcgtctc ggtgtacggc gccgcggcgc gaacatcggg atccagcgcc
     1801 ctggccttgc tcgcgagcgc ccgccagtcg gccagcccgt tgcccgtgtc gtccatcagc
     1861 tgcacgtgcg cgatggcctt gagcatcttg tcggtgacct ccttctggaa gccgttcatc
     1921 accgacagca cgatgatcaa cgccgcgacc cccagcccga tgcccagcat cgagaccagg
     1981 gagatgaatg aaatgaaggc gttgcgccgc ccagcgcggc cggcgcgtgt gtagcgccag
     2041 ccaatcaacc actcgaatgg caccggaatt cctcgcccgt gaaggggcat gccggggcat
     2101 tctaggcggg ccgcaggacg ggctccgcgt cggacaatgc cgcaatcggc gcacgcgacc
     2161 gtgccgcctg gccggactgg ccctaacatt ggcgccgaac cacctgggcg cgctgggagc
     2221 gccttgagag aatccatcga atgaaagctc cggacttcgc aggcgcctcc cgggaggtgc
     2281 tctcgttcct gcaccagcgc atcggcatgg acctgtggct ggtcagccgc gccgacaacg
     2341 aggactgggt cgtgctcgcg gccgaggacc acggctacgg cgtcaaggaa ggcgacgtct
     2401 tccgctgggc cgacacgttc tgctcgcgca tggtgcgcga cgagggcccg cgcgtggccc
     2461 cgcgcgtcga cgaagtgccc gcctacgccg ccctgccctt cgcgcgccag ctgcccatcg
     2521 gcgcctacgt cggcgtgccg ctgtgcgacg cccagggcca cctgttcggc acgctgtgcg
     2581 gcatcaatcc gcggccgatg ccctccagcc tcgccaacga gcaggcgatg gtgcaggtgc
     2641 tgggcgacat gctcagcggc gtgctcagcg ccgagctgca ggccagcacc ctcgcccgcg
     2701 aggccgaggc cgcgcgctcc gaggcgctca ccgacccgct caccggcctg gccaaccggc
     2761 gcggctggga gacggcgctg gccgccgagg aggaacgctg ccggcgctac ggcgccactg
     2821 cctgcgtggt ggccatcgag ctggacggcc tgaagggtcc ggcgccgcgc gaggtctgcg
     2881 aggccgtgct cgtgcgcgct gcccaggccc tgcgccagac ggtgcgcaag cccgacaccc
     2941 tcgcccgcct cgacgaggac cgcttcatgg tgctgggcgt cgagtgcacc accgccgagg
     3001 ccatggccct gctgcgccgc ctcgagcaga gcgtgcagac ggcggacctg tcggccgcgc
     3061 tcggcatggc catgcgcaat cgcagcagcg gcctgttcga caccttcgac cgcgcggtcg
     3121 acgcgatggt ggaacaccgg gacgcacgcg cccgggcctg aacgtgccgc gcgcgggacg
     3181 cgcgaagcgt ccgcgggata atctcggcat gcatctcgtg atcccctccg catcggcgct
     3241 gtccgatgcc gccgtgcagg cgctcggcac cctgagcctg ccgaacctgg agcgcctgct
     3301 cgcgcgctgg gcgccgcagg aggatgcgag caccgccgat attcccgacg ccgacgaata
     3361 cgcgctgtcg ctgccgcacg agcgcctgct ggcgcgcctg cgcgggtggc cggtggtcga
     3421 cggcctgctg ccgttcgcgg ccgattccgc acgccgcgac gggctcgcgc ccgagccggg
     3481 cagcggctgg gggctcatca cgccggcgca ctggcacgtc ggccgcgacc aggtcaacct
     3541 cgtcgacccg gcgctgctcg atctggacga agacgaggcg cgcacgctgc atgcgacgct
     3601 ggctccgctg ttcaccgaac tcggctgggc ctggcactgg gccggcccga cgcgctggta
     3661 cgtgagccat ccgtcgctgg ccgggctgcc caccgcgtcc atcgaccgcg tgatcggccg
     3721 caacgtcgac ctctggctca acgaccaccc cggcgtgatg ctggtgcggc gcctgcagag
     3781 cgaggtgcag atgctgctgt acgtgcaccc gctcaacgac gcccgcgagg aacgcggcgc
     3841 gatgagcgtg aactcgttct ggctcggcgg cacggggccg gcccagccgg ccgacaaggt
     3901 gctgcccgcc gacgtggtcg tcgacgatcg cctgcgcgcg ccgctgctgg gcgacgactg
     3961 gagcgcctgg gccgaggcct ggcacgcact cgacgccggg ccgctgcgcg cgctcgcgga
     4021 atcgcaagcc gccggcgcgc gcctgacgct ggccggcgaa cgccaggcgc gcagctggca
     4081 gcccgccgcg cgcccgtggt ggaagcggct ggtcggcggc ggcggcgcgt cggtgcaatc
     4141 gacgctggag gcgctgtgag cccgcaaccc cgcctccaca cgcgcgacgt cccgccgcgc
     4201 accgccttcg cgctggagca ggccggcgtg catccgctgc tcgcgcgcct gttcgccgcg
     4261 cgcggcgtgc gcagcgccga cgagctcgac gacgcgatgg gccagctgct gccgccctcc
     4321 acgctgcgcg gcagcgccga ggccgcgcgc ctgctggccg acgccatcga cgccaaccag
     4381 cgcatcgtgg tcgtcgccga ctacgactgc gacggcgcca ccgcctgcgc cgtcgcgctg
     4441 cgcgggctgc agatgctggg cgcgagcagc gcgcggctga gctacgtggt gcccgaccgg
     4501 cagctgcacg gctacggcct gacacccacc atcgtcgacc tcgcatgcgg cgacgacggc
     4561 cccgacgacg cgcggccctt cggcgccgag cgtccgccca agccggacct gctcgtcacc
     4621 gtggacaacg gcatcgcgag cctcgcgggc gtggcgcatg cgcgcgcacg aggcatcgcc
     4681 gtgctcgtca ccgaccacca cctgcccgcg ctcgtcgacg acaaggtggt gctgccggag
     4741 gccgacgtca tcgtcaaccc gaaccagccc gagtgcacgt tcgcgagcaa ggcgatggcc
     4801 ggcgtcggcg tcatgttcta cacgctgctg gccacgcgcg ccgagctgcg cgagcgcggc
     4861 cgcttcgacg ccgcgacgca gccgcggctg gatgcgctgc tcgatctcgt cgcgctcggc
     4921 acggtggccg acgtggtcaa gctcgacccg aacaaccggc gcctcgtcgc gcaaggcctc
     4981 aagcgcatcc gcgccggccg catgcagccg ggcatcgccg cgctgttcca cgtcgcggcg
     5041 cgcaagcacg agcgcgccag cgcgttcgac ttcggcttcg cgctcggccc gcgcctgaac
     5101 gccgccgggc ggctggccga catgacgctg ggcatcgagt gcctgctgac cgacgacccg
     5161 acgcgcgccg cgcagctcgc cggcgagctc gatcgcatca accgggagcg gcgcgacgtc
     5221 gaggccggca tgcgcgagca ggccgaggcg ctgatggagc agatcatgcc caccggcgcg
     5281 ccgccggcgg cgctggcact gttcgacgac ggcttccacg aaggcgtggt gggcatcgtc
     5341 gccggccgcg tgaaggatcg gctgcatcgg ccgaccttcg tcttcgcggt gggcgccgac
     5401 ggcttgctca agggctcggg ccggtcgatc gccggcttcc acctgcgcga cgcgctcgac
     5461 ctcgtcacca agcgccaccc cggcgtgctg cgcaagttcg gcggccactc gatggccgcg
     5521 ggctgcacga tcgcgcgcga ggacttcgag accttcgcgc aggccttcac gcaggtcgcg
     5581 gaggaatggc tcagcccctc ggcgctctcg cgcgtgctcg ccaccgacgg cccgctcgcg
     5641 ctcgaatggt tcaacaccgc caccgtcgcc acgctggacg cgcaggtgtg gggccagggc
     5701 ttcgaggcgc ccaccttctg cgacgaggtc gacgtggtgc agcagcgcct ggtcggcgag
     5761 aggcacctga agctgcgcgt gcgccacgcc ggccagctgc gcgacgcgat ctggttcggc
     5821 cgcagcgagc ccgtgcccga acgcctgcgc atcgcctacc ggctcagcat cgacgagttc
     5881 caggggcagc agcggctgca gatggtggtg gaagcggcgg gctgatacgg tcctcgcggc
     5941 gcgggaaagg gtggtacaac gccgactcgc ggccaggcgc gacagacgcc gccgcaagag
     6001 ggagaaccat gcatcgatcc gtttgccgcg cggcgctcgc cgttgcactt tgcctgccgg
     6061 tgcttgccgg cgccagcaag tcgtccgtcg acctggggcc gaccggcaat ccgccgccgc
     6121 gcctggaccc gctgctcgag cccatgctgc gacggcccac ctacgcgtcg atggtgctgt
     6181 cgccggacgg caagcacatc gccgccgtgg gcttcaacgg cgccggcacc aattcggccg
     6241 tcgtcatgct cgacgccgac acgctggaag gcaacctgat cgtccagccg cgcccgtgga
     6301 cggtgaaggg ctaccgaccc tacatccgca atccgcgcaa cgttgcctgg ttgagcgatt
     6361 cgaagatcgc cgtgaacttc acggtggcgg atgccgcgat cttcggcatc gacggcgcac
     6421 cgggcacgga cctgatgcag ggctacgtgc aaccgttccg cgacgcggcc ggcaagccga
     6481 cgtcctggca cctcgtcgtg cgcgacctcg cccggcgcga gttctcgcgc atgaacatcg
     6541 aggacggcac gaacacgtcc tacgacctgg acatcgacgg caaggccgtg ggctgggcga
     6601 gcgacgccag cggcgacatc cgcgtcgcgc agacgatcga taccgccttt ttcagcgacc
     6661 actcgcgcat catcacctgg tatcgaccgt ccaccagcga caaatgggtc aaggtcgacg
     6721 aacgctccgt gctggacgag gaattccatc ctttgttcgt cccgaaccgg ccggatcgga
     6781 tcgtcgtcca ggcgcgcaac ggccacgaca tgctggccat ctgggactac gacgtcgcca
     6841 agcacgcctt cgcggacctg ctcgcgggca ccggcaccga ggacatcgcc ggcgtcgtcc
     6901 tggacaacgg gctggacaac ttcaacgagg tcgcgaccga cggactcaag cgccagtcgc
     6961 actggttcga cacgcgctac gcccggctgc aggcgtcggt cgacgccagc atgccggacc
     7021 acgtcaacgt gatccagccc aatccgtccg atcgggtgct cgtctactcg tactcggacc
     7081 gggacccggg gcgctggtac ctgctgaaca ccaaggcgat gtcgatgaag ctcgtggctc
     7141 agcgcgtcca aggcatcgac cccgcgcgga tgcagccgat gcagacgctg cactatccgt
     7201 cgttcgatgg cctgtccgtg ccggcctacc tcacgctgcc tggcaagccg gccaagccgg
     7261 cgccgaccgt cgtgctgatc cacggcggtc cgcaactgcg cgatcgctgg cagttcgatg
     7321 ccgaggtgca gatcctcgcc gcccacggct acgcggtgtt ccagccgcag ttccgcggct
     7381 cctccggatt cggcaagcat ttcgaggagg cgggctacgg ccagtggggc caggcgatgc
     7441 aggaggacat cgacgccggc gtgaaatacc tcgtcgacca gaagatcgcc gatccgcagc
     7501 gactgtgcat cgtgggcgca agctacggag gctacgctgc actctgggcg ctggccaagg
     7561 atccgcagtt gttcaagtgc ggcgcgagcg tcgcgggcgt gtcggacctg cgccggatgc
     7621 tgcacgacga ctcggacatg agccacaacg ccgtcgcgcg tgagatcgtg cgtcatcgcc
     7681 tcggcgacgg cagcgagatg aaggcgacct tcgacagcgt ctcaccggtc aagcatgccg
     7741 accgcatcgt cgcgccgctg ctgctggtgc acggggacct cgaccagcgg gtgcctgtat
     7801 cgcacggcaa ggagatgcac gaggccctgg tcgatctcaa gaaggacgtc gtctggatcg
     7861 agttcccgga cgaacggcac ggcatcgcca agccgcagaa ccagcgcatc tacttcgacg
     7921 cgctgttcaa gctgctcgaa cgcacgatcg gcaagggcga gccgccgttt ccggctgcgt
     7981 cgccggccgc ccagaaccag gagattccac tgagcccggt ggactcgatc gatacgcctt
     8041 gaggcgccgc ggcgccgtca gtgcgccgac ggtgcgtact gccgcagccg cgccttccct
     8101 tgcgccgtca tcgcgaagcg gccgccctcg tcgaacgaca gaaggccgag ctggcgcagt
     8161 tcggccagtt cgcgccgggc ctgggccgtg gcgggttcgg gctcgccggc ggcggtcttg
     8221 aacagcaggt tgatctggcg ggggttgagc atcggaagca tggtggctcg gatcggaagt
     8281 ttggagagat ggccgggggg cgtagcaatt cccggtcccg gactttcccg ccacgttgaa
     8341 aggaatcgcc cgcgccgcgc gacgaaggac tatcctcgcc cgcacgcccc cttcccggcg
     8401 ccacgccatg acgcacctcc tgcccgcctc gacgccgcgc cgcggcctcg ccccttcctg
     8461 cctggccgcc gtcgcggttg cggccggcct gtcggcgcag ccctcgacgg ccgcggccgc
     8521 ggccgcgtgc gccgcgcgat ccggcccgac ggtgcccacc gtcgtcgagc tctacaccag
     8581 cgagggctgc agttcctgcc cgccggccga gcgctggctc ggcgccctga acggccgcga
     8641 cggcgtcgtc gccctcgcat tccacgtcga ctactgggac tacctcggct ggaaggatcg
     8701 cttcgcccag gcgctgttca cgcagcgcca gaacgcgtcg caacgcacca gcggagcgcg
     8761 cttcgcctat accccgcagg tgatcctgga cagccgtgac gcgccggcct ggagcacgct
     8821 gccggcgacc gccctgcagt cgcgcgcgct cgccaccgtc gcgctggcgc tggcgcgcga
     8881 cgacggcggg ctctcgctga cggtgacgcc cggccccggc gcgccggcgg ccctgtcggg
     8941 ctatgtggcg gtggtcgacg acgggctgcg cacccaggtg ggcgcaggcg agaaccgtgg
     9001 cgcgacgctg cggcaggacg cggtcgtgcg cgagctgctg ccgtggactc tggccggccg
     9061 ccaggcggcg acgctgcgct tctcgccaag cgccgccccc gaggccggcg cggcgcgcca
     9121 ctgggtggcg gtcgccaccg acgggccgta cggcaggccg gtgcaggcgc tcacgctcgc
     9181 ctgcgcgcgc tgaggtgcgc acggggggaa accccgtcca aaaaccgacc gcacggtcag
     9241 ggaatcgtga ttttcgccag cggcccgggg ctcgaaatca ggtacgctgt cggctgcgaa
     9301 ccgccacggt gaccccgtcg gcgctcgccc tgttccggcc ggccgcggca cacccaccgc
     9361 gcgacctgtc gattcgcagc tatctttggc gcccgccacc gcgggcgcgg agcattcagc
     9421 cgatgaccca gcacgacccc ttgagtctgc acgtcccgga gcccacgggg cgcccgggct
     9481 gcgcgaccga cttctcgtac ctccacgtct cgccggccgg cgaggtccgc cgcccgccgg
     9541 tggacacgga tccgagatcc accaacgacc tcgcgttctc gctggtgcgc gtcctggacg
     9601 acgacggcaa cgcggtcggc gcctgggccc cggaggtcag cgacgagctg ctgcgcaccg
     9661 gcatgaaggc catgatgaag acgcgcatct tcgacgcgcg catgctcatc ggccagcgcc
     9721 agaagaagat gtccttctac atgcagtcgc tcggcgagga ggccatcggc tcggcccacg
     9781 cgctggcact ggaagaaggc gacatgtgct ttcccacata ccgccagcaa agcctgctga
     9841 tcgtccggga ctatccgctg gtcgacatga tctgccagct gctgtccaac gagcgcgatc
     9901 cgctcaaggg ccgccagctg ccggtgatgt actcggtcaa ggccaagggc ttcttctcga
     9961 tctcgggcaa cctcgccacg cagtacatcc aggccgtggg ctgggcgatg gcctcggcca
    10021 tcaagaacga caccaggatc gcgtccgcct ggatcggcga cggcgccacc gccgagtccg
    10081 acttcaacac cgcgctgacc ttcgcgcacg tctacaacgc gccggtcatc ctgaacgtcg
    10141 tcaacaacca atgggcgatc tcgacgttcc aggcgatcgc cggcggcgag cacaccacct
    10201 tcgccgcgcg cggcgtgggc tcgggcatcg cctcgctgcg cgtggacggc aacgacttcc
    10261 tggcggtcta cgccgcctcg aagtgggccg ccgagcgcgc gcgccgcaac ctcggcccga
    10321 cgctgatcga gtgggtcacc taccgcgccg gcccgcactc cacgtccgac gatcccagca
    10381 agtaccgccc cgccgacgac gccacgcgct tcccgctggg cgatccgatc ggccgcctca
    10441 agcagcacct gatcaagcgc ggctgctggt cggaggccga gcacgaggag gtgcagaagg
    10501 cgctggaggc cgaggtggcc gccgcgcaga aggaggccga gtcctacggc acgctcaacg
    10561 acggccgcat cccgagcgcc gcctcgatgt tcgaggacgt ctacaaggag atgccggaac
    10621 acctgcgtcg ccaacgccag gagctggggg tatgaaggcc ccgctcgctg cactcgccgc
    10681 ccccgagggg ctggccggct tgctccggag cggccgcgcg cgcgccggct gcccattcag
    10741 cggacgagct tcgctcgccc gtgaaggact ctgatcatgg aaatgcaaga caagaagact
    10801 ctggcggccc agccccaagg cccgaccgcc ccgatgacga tgatccaggc gctgcgttcg
    10861 gccatggacg tgatgctgga gcgcgacccc aacgtggtca tcttcggcca ggacgtgggc
    10921 tacttcggcg gcgtgttccg ctgcaccgag ggcctgcaga agaagtacgg caagcagcgc
    10981 gtgttcgacg cgccgatctc cgaaggcggc atcgtcggcg ccgcggtggg catgggcgcg
    11041 tacggcctgc gcccggtcgt cgagatccag ttcgccgact acttctaccc ggcgtccgac
    11101 cagatcgtgt ccgaggcggc gcgcctgcgc taccgctctg cgggcgactt cacggcgccg
    11161 atgacgatcc gcatgccctg cggcggcggc atctacggcg gccagaccca cagccagtcg
    11221 cccgaggcga tgttcacgca cgtgtgcggc ctgcgcacgg tgatgccgtc caacccctac
    11281 gacgccaagg gcctgctgat ctcgtgcatc gagaacgacg acccggtgat cttcctggaa
    11341 cccaagcgc
//
LOCUS       DATCRO010000737         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_503534, whole genome shotgun sequence.
ACCESSION   DATCRO010000737 DATCRO010000000
VERSION     DATCRO010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503534"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..502)
                     /gene="glnK"
                     /locus_tag="VIN75_19990"
     CDS             complement(164..502)
                     /gene="glnK"
                     /locus_tag="VIN75_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8976505.1"
                     /translation="MKMVTAVVKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVDDSLVDQVIEAIERSARTGKIGDGKIFVSPLEQVVRI
                     RTGETGKDAL"
     gene            complement(584..1429)
                     /locus_tag="VIN75_19995"
     CDS             complement(584..1429)
                     /locus_tag="VIN75_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY8976506.1"
                     /translation="MKKSALATVAVLAAIGAPAIAFADDAPAGTAPAADASPITYNIG
                     VVSDYRYRGISQSKLKPALQGGVDFASGPFYVGTWASTIGWVKDSMVSYNRANASTPG
                     FVPVEEKGAPVEWDLYGGTKGDIIKDTLSYDVGVLAYVYLGQKFHDIGLANPDTGEVY
                     GALTYGVFTAKVSISATPLFGVPDSKGSQYYDLSANFDLGSGFTLTPDIGYQNVAGSY
                     AKNLGLPSYSYGAYSVTLAKDFGKGLSASLQVIATDADKTDYVTSGGKFTGRTGVVAG
                     VKYSF"
     gene            1936..3504
                     /locus_tag="VIN75_20000"
     CDS             1936..3504
                     /locus_tag="VIN75_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY8976507.1"
                     /translation="MTLAVIHTRALVGLHAPDVVVEVHLANGLPSFTLVGLADTEVKE
                     ARERVRAALSQSGFAFPHNQRITVNLAPADLPKESGRFDLPIALGVLAAQGLLDAARL
                     ARCEFAGELSLAGELRPVRGALALALAVRESGCARRLVLPAASAAEAARVQGVDVRSA
                     RNLGEVVQAFLPGDGAMAGARELPGPSTDGDVAPPPLADLADVKGQAGARRALEVAAA
                     GAHGLLLIGPPGAGKSMLAERLAGLLPPMTPAEALASAALLSVSTQGLDARRFGQRPV
                     RAPHHSASAVALVGGGSPPRPGEISLAHAGVLFLDELPEFPRSALEALREPLETGRIT
                     ISRAAFQAQYPARFQLVAAMNPCPCGWLGAFATSGRQCTCHGEAVRRYQARLSGPLLD
                     RIDMQIEVPALRPSELLPAARAPASPGDAPPPEASAPVAARVHAARERQLARQGTPNA
                     LLAAGQVETGLRLAEPARALLEQVAERLGWSARSLHRVAKVARTVADLAGAPDVEAAH
                     MAEAVQYRRGLPGG"
     gene            3721..4896
                     /locus_tag="VIN75_20005"
     CDS             3721..4896
                     /locus_tag="VIN75_20005"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8976508.1"
                     /translation="MNESPSHHHARIGIVGTGFVADLYMRSLKTFPHIAIAAAYDRDA
                     ARLQAFCSHWGVPPAASLDALLAAPGLELVLNLTNPGSHFEVSRACLAAGRHVYSEKP
                     LATDMDDARTLVDLARERGLMITSAPCSLLGEAAQTLWASVRRHDAGRPLLVYAELDD
                     DYVPTAPYRKWVSESGAPWPWRDEFEVGCTVEHAGYYLSWLIAMFGNVTTVVAASADV
                     IPNKLPGGGAAAPDWSCATLFFESGVVGRLTCSIVAPHDHALRVVGERGTIEIDECWA
                     NGAPVRVRRRHVVRRRQFDSPFARRVRVGTATDPKVGRRGAASMNFALGPAEMLASLR
                     EKRPCRLGADFALHFNEVTLAIQNSRAERGVQHMTTRCAPVQPQPWTLAAAPWKPQP"
     gene            4893..>5159
                     /locus_tag="VIN75_20010"
     CDS             4893..>5159
                     /locus_tag="VIN75_20010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8976509.1"
                     /translation="MSGARTWRFGVVGTGAMSATMMGCFGLEPRVEVAAVASADVARA
                     RAFADRFGIAHAHAGLDALLARDDIDAVYIATTNEKHKAQTLAAA"
BASE COUNT          646 a         1831 c         1931 g          751 t
ORIGIN      
        1 gcgggcgccg aggccgcggc gtcggccgcg agcgacggcg tcgcggagaa cccgaggacc
       61 gcgaggccca gggcgaggga agcgagaagc tttctcatgg tggcagagct ttcaggagtt
      121 ggaagcgtgt ggtgtcggaa acgtgcggaa gtcgcgcggc gcctcagagg gcgtccttgc
      181 cggtctcgcc ggtacggatg cgcaccacct gctcgagcgg cgagacgaag atctttccgt
      241 cgccgatctt tccggtgcgc gcggagcgct cgatggcctc gatgacctgg tcgacgagcg
      301 aatcgtcgac ggcggcttcg atcttcacct tgggcaggaa gtccacgacg tattcggcgc
      361 cgcggtaaag ctcggtgtgg cccttctggc ggccgaagcc cttcacttcg gtcaccgtga
      421 tgccctgcac gccgatggcg ctcagggcct cacgcacttc gtcgagcttg aagggcttca
      481 cgacggcagt caccatcttc atggtctcat ctccaggctg gcggacgccc tcgcgtccgg
      541 ttcttgtgaa ctggggtggc ccggttcgcg ccgggccggg atgtcagaac gagtacttca
      601 cgccggcgac gacgccggtg cggccggtga acttgccgcc ggaggtgacg tagtcggtct
      661 tgtcggcgtc ggtggcgatg acctgcagcg acgcgctcag gcccttgccg aagtccttgg
      721 ccagcgtcac ggagtacgcg ccgtacgagt agctcggcag gcccaggttc ttggcgtagg
      781 agccggcgac gttctggtag ccgatgtcgg gcgtcagcgt gaagcccgag ccgaggtcga
      841 agttcgcgct caggtcgtag tactggctgc ccttgctgtc gggcacgccg aacagcggcg
      901 tcgcgctgat cgagaccttg gccgtgaaca cgccgtaggt cagcgcgccg tagacctcgc
      961 cggtatccgg gttggccagg ccgatgtcgt ggaacttctg gccgagatag acgtacgcga
     1021 gcacgccgac gtcgtacgac agcgtgtcct tgatgatgtc gcccttggtg ccgccgtaca
     1081 ggtcccattc gaccggggcg cccttttcct cgaccgggac gaagcccggg gtgctcgcgt
     1141 tggcgcggtt gtacgacacc atcgagtcct tgacccagcc gatggtcgag gcccaggtgc
     1201 ccacgtagaa cgggccgctc gcgaagtcga cgccgccttg cagcgccggc ttgagcttgc
     1261 tttgcgagat gccgcggtag cggtagtcgg agacgacgcc gatgttgtag gtgatcgggc
     1321 tcgcgtcggc cgcgggtgcg gtaccggcgg gcgcgtcgtc ggcgaaggcg atcgcgggcg
     1381 cgccgatggc ggcgaggacg gcgacggtgg caagcgcgct tttcttcatc aggaaactcc
     1441 ggtggtctgg atgtggtggg tgggtgggat ctcttgtcgg acaccgctga cgcaggttcc
     1501 gtgccacgcg gtcgagctcg caagctcgcc gaaggctgct tcgcgcgtgc gcgcgcgatg
     1561 cgcggcggac gcgcgcgcct tggtccggtg gcttgcggac cgcggaccgc aggcggcgcc
     1621 gtgcggcgcg cgccgggcga cgccgctgcg cgcatgaccg cttgcccgga tcggagtcgc
     1681 accaagccgg tgcatccgcg cgacggccat gcacgccggt cgcactccga tggcgcgtcc
     1741 aaacgcgccg aaaccgcggc ccgggcgccg gtggtgcgtg ccatgcgctc cgacaatgga
     1801 tgacgcccag gccagaccgt ccggagcacg cctggcgccc ggatccgaat tggggccatg
     1861 caagtcgcgc gccagcttcg tgccgcgatg cgaggctcaa ctctggcccc tctttccaaa
     1921 ccttcgccgc cccgcatgac cctcgccgtc atccacaccc gcgccctcgt tggcctgcac
     1981 gcgcccgacg tcgtcgtcga ggtccacctg gccaacggcc tgcccagctt cacgctggtg
     2041 ggcctggccg acaccgaggt caaggaggcg cgcgagcgcg tgcgggcggc gctgtcgcag
     2101 agcggctttg cgttcccgca caaccagcgc atcaccgtga acctcgctcc ggccgacctg
     2161 cccaaggaat cggggcgctt cgatcttccg atcgcgctgg gcgtgctcgc cgcgcagggc
     2221 ctgctcgatg cggcgcggct cgcgcgctgc gagttcgcgg gcgagctctc gctggccggc
     2281 gaattgcggc ccgtgcgcgg cgcgctcgcg ctggcgctgg ccgtgcgcga gagcggctgc
     2341 gcgcgccggc tggtgctgcc ggcggcaagc gcggcggagg cggcacgggt gcagggcgtg
     2401 gacgtgcgct cggcgcgcaa tctcggcgag gtcgtgcagg cgttcctgcc cggtgacggc
     2461 gcgatggccg gcgcgcgcga gctgccgggt ccgtcgacgg acggcgacgt tgcgccgccc
     2521 ccgctggcgg acctggcgga cgtcaagggc caggccggcg cccgccgcgc gctcgaggtc
     2581 gcggccgccg gcgcgcacgg gctgctgctg atcgggccac ccggcgcggg caagtcgatg
     2641 ctggccgagc gcctggcggg cctgttgccg ccgatgacgc cagccgaggc cctggccagc
     2701 gcggcgttgc tgagcgtgtc gacgcagggg ctcgacgccc gccgcttcgg ccagcgtccg
     2761 gtgcgcgccc cgcaccacag cgccagcgcg gtggcgctcg tgggcggcgg ctcgccgcca
     2821 cgtccgggcg agatctcgct ggcccacgcg ggcgtgctgt tcctggacga gctgccggaa
     2881 ttcccgcggt ccgcgctcga ggcgctgcgc gagccgctgg agacgggccg catcacgatc
     2941 tcgcgcgcgg cgttccaggc gcaatacccg gcgcgcttcc agctggtggc ggcgatgaat
     3001 ccatgcccct gtgggtggct gggcgcgttc gcgacgagcg ggcggcagtg cacctgccac
     3061 ggcgaggcgg tgcggcgcta ccaggcgcgg ctgtcggggc cgctgctcga tcgcatcgac
     3121 atgcagatcg aagtgcccgc gctgcggccg tccgagctgc tgccggcggc cagggcccct
     3181 gcctcgccgg gcgacgcgcc gccgcccgag gcgtccgcgc ccgtggcggc gcgcgtgcac
     3241 gccgcgcgcg agcgccagct ggcgcgccag ggcacgccca acgcgctgct ggcggccggc
     3301 caggtcgaga ccggcctgcg gctggccgag ccggcgcgcg cgctgctgga acaggtggcc
     3361 gagcgcctgg gctggtcggc gcgcagcctg caccgggtgg ccaaggtggc gcgcacggtg
     3421 gccgacctcg ccggcgcgcc cgacgtcgaa gcggcgcaca tggccgaggc ggtgcagtac
     3481 cggcgcgggc tgccgggcgg gtgatgccgc gcgcggcatc cccggcccat cgagaggcac
     3541 tgcccttggc cgccagggcc ctggtgcata ctgcgccgca cccgcggcga ccgcccccgg
     3601 gcgcgccggg ccgctgcggc cgggctcgcc atttgccaag tcgcgcctcg ggtccggacc
     3661 gccgctccat cgcgcggcgc gcccaggcat gcccggacgg cagatctcct cctcgagtcg
     3721 atgaacgaat caccttccca ccaccacgcg cgcatcggca tcgtcggcac cggcttcgtc
     3781 gccgacctgt acatgcggtc gctgaagacc tttccgcaca tcgcgatcgc cgccgcctac
     3841 gaccgcgacg cggcccgcct gcaggcgttc tgcagccact ggggcgtgcc gccggcggcg
     3901 tcgctggatg cgctgctggc cgcgcccggc ctggagctgg tgctgaacct gaccaacccc
     3961 ggctcgcatt tcgaggtgag ccgcgcctgc ctggccgccg ggcgccacgt ctattccgag
     4021 aagccgctgg ccaccgacat ggacgacgcg cgcacgctgg tcgacctggc acgcgagcgc
     4081 ggactgatga tcacgtcggc cccttgcagc ctgctgggcg aggcggcgca gacgctgtgg
     4141 gccagcgtgc ggcgccacga tgcgggcagg ccgctgctgg tctacgccga gctcgatgac
     4201 gactacgtgc cgacggcgcc gtatcgcaag tgggtgagcg aatcgggcgc gccctggccc
     4261 tggcgcgacg agttcgaggt gggctgcacc gtcgaacacg ccggctacta cctgtcgtgg
     4321 ctgatcgcga tgttcggcaa cgtcacgacc gtggtggcgg cgtcggccga cgtcatcccg
     4381 aacaagctgc ccgggggcgg cgcggccgcg ccggactggt cctgcgccac gctgttcttc
     4441 gagtccggcg tcgtcgggcg cctgacctgc agcatcgtcg cgccgcacga tcacgcgctg
     4501 cgcgtcgtgg gcgagcgcgg cacgatcgag atcgacgagt gctgggccaa cggcgcgccg
     4561 gtgcgcgtgc gccggcgcca cgtggttcgg cggcgccagt tcgacagccc gttcgcgcgt
     4621 cgcgtgcgcg tgggcacggc caccgacccg aaggtgggcc ggcgtggcgc cgcgtcgatg
     4681 aacttcgcgc tcgggccggc cgagatgctg gcgtcgctgc gggagaagcg cccgtgccgc
     4741 ctcggcgccg acttcgcgct gcacttcaac gaggtgacgc tggccatcca gaactcgcgc
     4801 gcggagcgcg gcgtgcagca catgacgacg cgttgcgcgc cggtgcagcc gcagccctgg
     4861 acgctcgcgg ccgcgccttg gaagccgcag ccgtgagcgg cgcgcgcacc tggcgcttcg
     4921 gcgtcgtcgg caccggggcg atgtcggcca cgatgatggg ttgcttcggc ctcgagccgc
     4981 gcgtcgaggt cgcggccgtc gcctcggcgg acgtggcgcg ggcccgcgcg ttcgcggacc
     5041 gcttcggcat cgcgcacgcg cacgccggcc tggacgcgct gctggcgcgc gacgacatcg
     5101 acgccgtcta catcgccacc accaacgaga agcacaaggc tcagacgctc gcggccgcc
//
LOCUS       DATCRO010000738         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_64531, whole genome shotgun sequence.
ACCESSION   DATCRO010000738 DATCRO010000000
VERSION     DATCRO010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64531"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /gene="ispH"
                     /locus_tag="VIN75_20015"
     CDS             complement(<1..717)
                     /gene="ispH"
                     /locus_tag="VIN75_20015"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8976510.1"
                     /translation="MSEIQVGTVGEDGKFVEEVLLAQPRGFCAGVDRAIEIVERALEK
                     FGRPIYVRHEIVHNTFVVNDLKNKGAIFIEDLADVPPGATLVFSAHGVSKAVREEAAQ
                     RGFSVFDATCPLVTKVHVEVSKLAREGYEFIMIGHKGHPEVEGTMGQLSDGIYLVEDV
                     PDVANVHPNDPSKLAVVTQTTLSVDDAREILEAVKARFPLIREPKKQDICYATQNRQD
                     AVKFMAPKVDIVIVVGSPTSS"
     gene            complement(717..1160)
                     /locus_tag="VIN75_20020"
     CDS             complement(717..1160)
                     /locus_tag="VIN75_20020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8976511.1"
                     /translation="MNIVQPDSFLTLHYRLSGPDGAEVVSTFNDKPATLSLGQGQLAP
                     AIEACLVGMAEGARMTWRLEAGAAYGERNPDMLQRVKRSLLAQHGDPDEQYRVGDVVE
                     FPTPDGAGKFAGVVREAGDDWLLFDFNHPLAGQPVTFEAQLIGVM"
     gene            1292..1966
                     /gene="radC"
                     /locus_tag="VIN75_20025"
     CDS             1292..1966
                     /gene="radC"
                     /locus_tag="VIN75_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8976512.1"
                     /translation="MSIKEMPVAARPREKLLAQGAQALADVELLALLLRTGAAGQNVL
                     QRAERLLERFDGLAGLLHASPAELARAGGVGPAQRAELAAVREIARRALAQQLREAPV
                     FDSPQKVKDFVALRLGALTHEVFGVLFLDSQHRLIEMQELFRGTLAQTSVYPREVLRQ
                     ALLLNAGAAILVHNHPSGVAEPSRADEVLTQSLTSALRVIDVRVLDHLVVGAGTVVSF
                     AERGLM"
BASE COUNT          280 a          717 c          730 g          322 t
ORIGIN      
        1 ggagctggtg gggctgccca cgacgatcac gatgtcgacc ttgggcgcca tgaacttcac
       61 cgcgtcctgc cggttctgcg tggcgtagca gatgtcctgc ttcttgggct cgcggatcag
      121 cgggaagcgc gccttgacgg cctccaggat ctcgcgtgcg tcgtccaccg acagcgtggt
      181 ctgggtgacg accgccagct tgctggggtc gttcggatgc acgttggcga cgtcgggcac
      241 gtcctcgacg agatagatgc cgtcgctcag ctggcccatc gtgccctcga cctcggggtg
      301 tcccttgtgg ccgatcatga tgaactcgta gccctcgcgc gccagcttcg agacctccac
      361 gtgcaccttg gtcaccagcg ggcaggtggc atcgaacacc gagaatcccc gctgcgcggc
      421 ctcctcgcgc acggccttgc tcacgccgtg ggccgagaac acgagcgtgg cgccgggcgg
      481 cacgtcggcg agatcctcga tgaagatcgc gcccttgttc ttcaggtcgt tgacgacgaa
      541 ggtgttgtgc acgatctcgt ggcgcacgta gatcggccgg ccgaacttct cgagcgcgcg
      601 ctcgacgatc tcgatggcgc gatcgacgcc cgcgcagaag ccgcgcggct gcgccagcag
      661 cacttcctcg acgaacttgc cgtcctcgcc cacggtgccc acctggattt cgctcatcac
      721 atcaccccga tcagctgggc ttcgaaggtg accggctggc cggccagcgg gtggttgaag
      781 tcgaacagca gccagtcgtc gccggcctcg cgcacgacgc cggcgaactt gcccgcgccg
      841 tcgggcgtgg ggaactcgac cacgtcgccg acgcggtact gctcgtccgg atcgccatgc
      901 tgcgcgagca ggctgcgctt gacccgctgc agcatgtcgg ggttgcgctc gccgtacgcg
      961 gcgcccgcct ccaggcgcca ggtcatgcgc gcgccctccg ccatgccgac caggcaggcc
     1021 tcgatggccg gcgccagctg gccctgcccc agcgacagcg tggcgggctt gtcgttgaac
     1081 gtgctgacga cctcggcgcc atcggggccg ctgaggcggt agtgcaacgt caggaacgag
     1141 tcgggctgga cgatgttcac gggaaatcag gcagttggac gggcacggcc cgccgggttg
     1201 ggcgcgacac tgggcgggcc ggcgcgcggg ggatgcgcgg cggcgccttc cccaggacga
     1261 aaccccgatt ttacccgccc gcccagccgc catgtcgatc aaggaaatgc ccgtcgccgc
     1321 ccgcccgcgc gagaagctcc tggcccaggg cgcccaggcg ctcgccgacg tcgaactgct
     1381 ggccctgctg ctgcgcaccg gcgccgccgg ccagaacgtc ctgcagcgcg ccgagcgcct
     1441 gctcgaacgc ttcgacggcc tggccggcct gctgcacgcc tccccggccg agctggcgcg
     1501 tgccggcggc gtgggcccgg cccagcgcgc cgagctggcg gcggtgcgcg agatcgcgcg
     1561 gcgcgcgctg gcccagcagt tgcgcgaggc gccggtgttc gactcgccgc agaaagtgaa
     1621 ggacttcgtc gcgctgcggc tgggcgcgct cacgcacgag gtcttcggcg tgctgttcct
     1681 cgattcgcag caccgcctga tcgagatgca ggagctgttc cgcggcacgc tggcgcagac
     1741 cagcgtctac ccgcgcgagg tgctgcgcca ggcgctgctg ctcaacgccg gcgcggcgat
     1801 cctcgtgcac aaccacccgt ccggcgtggc ggagccgtcg cgcgccgacg aggtgctgac
     1861 gcagtcgctg acgtcggcgt tgcgggtgat cgacgtgcgg gtgctggacc acctggtggt
     1921 gggggcgggg acggtggtca gcttcgcgga gcgggggctg atgtgagccg tcgccatcgg
     1981 tcgcagaagt cctcgtcccg cgatcacgtg gcaggaccag ggaacttcct gctcaagcgg
     2041 ggcagggtc
//
LOCUS       DATCRO010000739         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_920069, whole genome shotgun sequence.
ACCESSION   DATCRO010000739 DATCRO010000000
VERSION     DATCRO010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920069"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..578
                     /locus_tag="VIN75_20030"
     CDS             48..578
                     /locus_tag="VIN75_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976513.1"
                     /translation="MSNDTQTPSPTIDSEALARLQAWQGRSETLADDITAAPGRALAA
                     TLDRDDPPPQAAPGEYTWSRDIVADDVLLFRYSALTFNGHRIHYDRRYVTEVEGYPGL
                     IVHGPLIATLLVDLLRRNVPGAQLARFEFRAVRPTFDIAPFRVHGKPGADGKTFDLWG
                     EDADGWLTMQATAVLA"
     gene            597..1778
                     /locus_tag="VIN75_20035"
     CDS             597..1778
                     /locus_tag="VIN75_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8976514.1"
                     /translation="MTRPLDGITVISLEHAIAAPFCTRQLADLGARVIKVERPGAGDF
                     ARAYDARVAGEASHFVWVNRSKESLTLDLKQPAALAVLQELLADADVLVQNLAPGAAA
                     RMGLGAQALHERHPRLIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTPEEPCK
                     SGNSIADIAAGMYAYTGILAALLQRGKTGRGSHIDVSMLEALAEWMGYPMYYAYDGAP
                     PPPRSAASHATIYPYGPFPAGDGGTVMLGLQNEREWRTFCEKVLLQTALATDPRFDSN
                     ARRNENREMLRAIILETFGALTTAQVLERLDTAQIANARMNDMAGLWAHPQLQARERW
                     RQVGSPAGDIPALLPAGRQSAFDYRMDPIPAVGEHTDAILRSLGRSEADIAALREAGA
                     V"
     gene            1775..>2130
                     /locus_tag="VIN75_20040"
     CDS             1775..>2130
                     /locus_tag="VIN75_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8976515.1"
                     /translation="MSAPLLSLARAFLFVPADRPERHARALATGAGGVIVDLEDAVAP
                     ERKVQARDQLGAAFAALPAEDRRRLLVRINAAGTPWHDDDCAQVGALAAQGMIAGIVL
                     PKAERAASAPTCAQSSS"
BASE COUNT          333 a          780 c          736 g          281 t
ORIGIN      
        1 tcgtcaaggc ctccggcgcc gcggtcgact gaacgacgaa gagacccatg agcaacgaca
       61 cacagactcc atctcccacc atcgacagcg aggcgctggc caggctgcag gcctggcagg
      121 gccgcagcga aacgctggcc gacgacatca ccgccgcacc ggggcgcgcg ctcgccgcca
      181 cgctcgaccg cgacgacccg ccgccgcagg ccgcgcctgg cgaatacacc tggtcgcgcg
      241 atatcgtcgc cgacgatgtg ctgctgttcc gctactcggc gctgaccttc aacggccacc
      301 gcattcatta cgatcgtcgc tacgtcaccg aggtggaggg ctatccgggc ctgatcgtgc
      361 acggcccgct gatcgcgacg ctgctggtcg acctgctgcg ccgcaacgtg ccgggcgcgc
      421 agctcgcgcg cttcgagttc cgcgcggtgc gcccgacctt cgacatcgcg ccgttccgcg
      481 tgcacggcaa gccgggcgcc gatggaaaga cgttcgatct gtggggcgag gacgccgacg
      541 gctggctcac catgcaggcc acggccgtgc tggcctgagc ggggagggaa gaacacatga
      601 caagacccct ggacggcatc accgtcatct cgctcgagca cgccatcgcc gcgccgttct
      661 gcacgcggca gctcgccgac ctcggtgcgc gcgtcatcaa ggtggagcgc cccggagcgg
      721 gcgacttcgc gcgcgcctac gacgcccgcg tggccggcga ggcctcgcac ttcgtgtggg
      781 tgaaccgctc gaaggaaagc ctcacgctcg acctcaagca gcccgccgcg ctggcggtgc
      841 tgcaggagct gctggccgat gccgacgtgc tggtgcagaa cctcgcgccc ggcgccgccg
      901 cgcgcatggg gctgggcgcg caggccctgc acgaaaggca cccgaggctc atcgtctgcg
      961 acatctcggg gtacggcgag gacggcccgt accgcgacaa gaaggcctac gacctgctga
     1021 tccagagcga ggccggcttt ctctcggtga cgggcacgcc cgaggagccc tgcaagtcgg
     1081 gcaactccat cgccgacatc gcggccggca tgtatgcgta caccggcatc cttgcggcgc
     1141 tgctccagcg cggaaagacg ggcaggggct cgcacatcga cgtgtcgatg ctcgaggcgc
     1201 tggccgagtg gatgggctac ccgatgtact acgcctacga cggcgctcca ccgccgccgc
     1261 gcagcgccgc ctcgcacgcg accatctatc cctacggtcc tttccccgcg ggtgacggcg
     1321 gcaccgtgat gctgggcctg cagaacgagc gcgaatggcg caccttctgc gagaaggtgc
     1381 tgctgcagac agcgctggcc accgacccgc gcttcgacag caacgcgcgg cgcaacgaga
     1441 accgcgagat gctgcgcgcc atcatcctcg agaccttcgg cgcgctgacg acggcgcagg
     1501 tgctggagcg gctcgacacc gcgcagatcg ccaacgctcg catgaacgac atggccggcc
     1561 tgtgggccca tccccaattg caggcgcgcg agcgctggcg tcaggtgggc tcgccggcgg
     1621 gcgacatacc ggccctgctg ccggcagggc gccagagcgc cttcgactac cgcatggacc
     1681 cgatcccggc ggtaggcgag cacaccgacg ccatcctgcg cagcctcggg cgcagcgagg
     1741 cggacatcgc ggcgctgcgc gaggcgggcg cggtgtgagc gcgccgctgc tttcgctggc
     1801 acgcgccttt ctcttcgtgc ccgccgaccg gcccgagcgc catgcgcgcg cgctggccac
     1861 cggggcgggc ggcgtgatcg tcgacctcga agacgccgtc gcgcccgagc gcaaggttca
     1921 agcgcgcgac caactgggtg ccgcgttcgc cgcgctgccc gccgaagacc ggcgacggct
     1981 gctggtgcgc atcaacgccg ccggcacgcc gtggcatgac gacgactgcg cgcaggtcgg
     2041 agcgctcgcc gcccagggga tgatcgccgg catcgtgcta ccgaaggcgg aacgggcggc
     2101 gagcgctccg acctgcgcgc agtcgtcgtc
//
LOCUS       DATCRO010000740         7825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_952859, whole genome shotgun sequence.
ACCESSION   DATCRO010000740 DATCRO010000000
VERSION     DATCRO010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952859"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VIN75_20045"
     CDS             <1..432
                     /locus_tag="VIN75_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976516.1"
                     /translation="AERRAVIVSGCDQLLEQAGVIIVRDRGYVDMLALLRHARCLASG
                     AEGHLLEEAGALGITTLTVGPEAGDDDEQAGRCDVAVGLDAQLAQRAVRVVNANDNRL
                     DDKAAGPDVWDGGATARVTKWLAHWLGYKYRTSRKGMETIG"
     gene            429..1313
                     /locus_tag="VIN75_20050"
     CDS             429..1313
                     /locus_tag="VIN75_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEY8976517.1"
                     /translation="MTSEQPGAAPGAIVNALTVDVEDYFQVSAMAPYIARSAWDTIEC
                     RVENNVERLLARFADNGAHATFFTLGWIAERHPAMIRRIVAAGHELASHGYGHQRATD
                     LTRAEFEEDITRAKRILEDAGGVEVRGYRAPSFSIDKRNLWAFDSLRNAGYAYSSSVY
                     PVHHDHYGMPDAPRFPYASVEGLTEIPISTVRAGGRNIPIGGGGYFRLLPYAASRWAI
                     ARFNREERRPAIFYMHPWEIDPGQPRVAGVDAKSRFRHYVSLHRTEGRLERLLQDFRW
                     DRVDRVFDVQARQSALAA"
     gene            1310..2452
                     /locus_tag="VIN75_20055"
     CDS             1310..2452
                     /locus_tag="VIN75_20055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8976518.1"
                     /translation="MNARTPIRVLHVLLSLEPGGLENGVVNVINRLDRERFASSVCCL
                     KHAGEFARRIEDPRVDIHELHWRGGNDPRLALRLAGLLRRLRPDIVHTRNAEPFFYGF
                     AGAKLARAQALVHSEHGRKFDDRPARFALQRWMSRHTDAIFAVSGQLKTDLVKHIGMP
                     EASVEVLHNGVDLSRFNVAAGAGAPSPARAALRRAWGVPESALVVGSVGRLVAVKNYA
                     LLLRAVAAGSLDIHLVLAGEGPERAALTALAASLGIASRVHLLGHSNDVDRVLAAFDV
                     FVLPSFSEGMSNTLLEAMAAGVPPVASEVGGNGEIVRDGVDGRLFPSDDEPALRACLE
                     ALCRDGAERARLGAAARERVLSTFDIRQMIERYERLYERVHARTVR"
     gene            2511..3806
                     /locus_tag="VIN75_20060"
     CDS             2511..3806
                     /locus_tag="VIN75_20060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8976519.1"
                     /translation="MNSTGGEPLVAAAARPAVPRAEASPAEGRLRVVVLTTFFPSPRD
                     PFRTPFLRNLVDAMAGDCDQELVIPVPRRPPIGPWRDAQPVPASETHAGWTLAHPRYL
                     AIPGLHWLSGLTYFLGTWRTLRALRRRHGRYVLHAHCAYPDVVGAALAAKVLGLPLVA
                     TAHGSDINISGRQWLLRPQIRWALRAARRVIAVSAPLAAAVSELTKLPAEGVRCIPCA
                     GYFPAVFRPRDAGERAALRQALGIAHDAQVVLFVGHLVPVKALDVLLRAWSTLLQFSS
                     VGAHLRLVLIGEGTLRDTLEQQARELGVADRVAFLGPKPQPVVADWIAAADLLTLPSH
                     AEGSPNVVVEALASGTPVVASRVGGVPDLVDDGVNGLLVPAGDAAALAQALGAALARE
                     WDAARISASIAHLTWPALARRNHDVLAAVATETAHAGAR"
     gene            3790..4482
                     /locus_tag="VIN75_20065"
     CDS             3790..4482
                     /locus_tag="VIN75_20065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8976520.1"
                     /translation="MLALARTLSKDLLQQAIFPDRFLWRLPKPRRAIALTFDDGPHPE
                     HTPAMLDVLARAGVRATFFVVGREVEKHPEVARRIVAEGHGIGGHSYDHTVMTTLDDA
                     ALVDDLAHCRRVIREATGLDTGLFRPPKGEVSLRSIRTACRAGYTLVHWTRTYSDYRQ
                     DGVDALTRRIDAEPPIAGDVLLFHDHNPYTVRALDSKLPQWRAKGLQFAPLNRPVEQP
                     GGSSKAGIGKTL"
     gene            5009..7267
                     /locus_tag="VIN75_20070"
     CDS             5009..7267
                     /locus_tag="VIN75_20070"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976521.1"
                     /translation="MNSMKFAAKSAVQLAALATFAAYGSAAQASTVTLTVKSGLGGVV
                     QSDPYGYISCTSAPQSSTCSANVTAGMAIALTETPLKGYVFAGWSGGTCSGTAATCYT
                     RVMATETITANFKSTAAAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPAPAPVGATTAPVVDFVDTTSAPTIGGENGAGGYLSIFGSNFGAASGM
                     GTTTKVYIGGVEVANYRYLGNAKVGAKLGLQQITVQVGSLGGAKVGVAQNIQVVVNGV
                     NSNVNNTFTPTAGKVWFVSLSGNDSTAVANDITHPYRYLQNMNTLTGAYFAMGTGDQV
                     VLRGGNWSDTNGVDSSWMKFGASQYARNGTPSAYISFTAYPGPVKGNAIEDVHYTTPA
                     GTSGGFQGPWSSIAGTSGEYIAISNLRMEVAGGANRDAAPINFQYTGGHWRAVNNEIG
                     PWVAGSSAILNAAGISGHGDGNVLLGNHIHNIAGLSDLQNHGIYADTTAQNWDVGYNW
                     VHDITGGSAIQFNDNQGGAGSYPMPHGGTWAGFIGIKIHNNWFENAAKYGINFNDQMS
                     TKAGTYSGMIWDNVVIGTALPPLRINSTQPTQKLWFAYNTMYNCMTTVSGSGNGYVRS
                     EGWSAQAGVNNVFFDNIFAYGPKTVSGTQYFANVGGTAASTTTYNFKRNLYFPNGQAP
                     SAASTIGDISALTADPLFVAATSSNFQTQSTSPARRAATQGLPSGMSVTTDFTGLVTR
                     GSPSDIGAYAAN"
     gene            7478..>7825
                     /locus_tag="VIN75_20075"
     CDS             7478..>7825
                     /locus_tag="VIN75_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976522.1"
                     /translation="MTQNSPRNRARAMATLGVLFALAGCGGGASPQSVTPTPSPTPSP
                     TPAPTPAPGTQTLTLNNVGSGSLTSTTNGASCSTATCSTELATNTVVALTAVPAADYT
                     FGGWGGGCSGTGTT"
BASE COUNT         1200 a         2955 c         2558 g         1112 t
ORIGIN      
        1 gccgagcgcc gcgccgtcat cgtctcgggc tgcgaccagc tgctggagca ggccggcgtg
       61 atcatcgtgc gtgaccgcgg ctacgtcgac atgctcgcgc tgctgcgcca cgcgcgctgc
      121 ctggccagcg gcgccgaagg ccacctgctg gaggaggcgg gcgccctggg catcaccacg
      181 ctgaccgtcg gccccgaggc cggcgacgac gacgaacagg ccgggcgctg cgacgtcgcg
      241 gtgggcctgg acgcgcagct cgcgcagcgc gccgtgcgcg tcgtcaacgc caacgacaac
      301 cgcctggacg acaaggccgc cggccccgac gtctgggacg gcggcgccac cgcgcgcgtg
      361 accaagtggc tggcccactg gctgggctac aagtaccgca ccagccgcaa gggcatggag
      421 accatcggat gacgtccgag caaccaggcg ccgcccccgg cgcgatcgtc aacgcgctga
      481 ccgtcgacgt cgaggactat ttccaggtct ccgcgatggc gccctacatc gcgcgcagcg
      541 cgtgggacac gatcgagtgc cgcgtcgaga acaacgtgga acgcctgctc gcgcgctttg
      601 ccgacaacgg cgcgcacgcc accttcttca cgctcggctg gatcgcggaa cgccatcccg
      661 cgatgatccg ccgcatcgtc gccgcgggcc acgagctggc cagccacggc tacggccacc
      721 agcgggccac cgacctgacg cgcgccgagt tcgaggagga catcactcgc gccaagcgca
      781 tcctggagga cgcgggcggc gtcgaggtgc gcggctaccg cgcccccagc ttctcgatcg
      841 acaagcgcaa cctgtgggcg ttcgacagcc tgcgcaacgc cggctatgcc tacagctcca
      901 gcgtctaccc ggtgcaccac gaccactacg gcatgcccga cgcgccgcgc ttcccgtacg
      961 cgtcggtcga gggcctgacc gagatcccga tctccaccgt gcgcgccggc ggccgcaaca
     1021 tccccatcgg cggcggcggc tacttccgcc tgctgcccta cgcggcctcg cgctgggcca
     1081 tcgcgcgctt caaccgcgag gagcggcggc cggcgatctt ctacatgcac ccgtgggaga
     1141 tcgacccggg ccagccgcgc gtggccggcg tggacgccaa gtcgcgcttc cgccattacg
     1201 tcagcctgca ccgcacggag ggccgcctgg agcgcctgct gcaggacttc cgctgggatc
     1261 gcgtggaccg ggtgttcgac gttcaggcgc gccagtccgc gctcgcggcg tgaacgccag
     1321 gacgccgatc cgggtcctcc acgttctcct gagcctggaa cccggcggcc tcgagaacgg
     1381 ggtcgtcaac gtcatcaacc gcctcgaccg cgagcgcttc gcgtcctccg tgtgctgcct
     1441 gaagcatgcg ggcgagttcg cccgccgcat cgaggatccg cgcgtggaca tccacgagct
     1501 gcactggcgc ggcggcaacg atccgcgcct ggcgctgcgc ctggcgggcc tgctgcgccg
     1561 cctgcggccc gacatcgtgc acacgcgcaa cgccgagccc ttcttctacg gcttcgccgg
     1621 cgccaagctg gcgcgcgccc aggcgctggt gcacagcgag cacgggcgca agttcgacga
     1681 ccgtcccgcg cgctttgcgc tgcagcgctg gatgagccgg cacaccgacg cgatcttcgc
     1741 ggtctcgggc cagctcaaga ccgacctcgt caagcacatc ggcatgcccg aggccagcgt
     1801 cgaggtgctg cacaacggcg tcgacctgag ccgcttcaat gtcgccgcgg gcgccggcgc
     1861 gccctcgccc gcccgcgcgg cgctgcgccg ggcatggggc gtgccggaaa gcgcgctggt
     1921 ggtgggcagc gtgggtcggc tggtggccgt caagaactac gcgctgctgc tgcgcgccgt
     1981 cgccgccggc agcctggaca tccatctcgt gctcgccggc gagggcccgg agcgcgccgc
     2041 gctgacggcg ctggccgcgt cgctgggcat cgcctcgcgc gtgcacctgc tgggccacag
     2101 caacgacgtc gaccgcgtgc tggccgcgtt cgacgtgttc gtgctgccgt cgttcagcga
     2161 gggcatgtcc aacacgctgc tcgaggcgat ggccgcgggc gtaccgccgg tggccagcga
     2221 ggtcggcggc aacggcgaga tcgtgcgcga cggcgtcgac ggccgcctgt tccccagcga
     2281 cgacgagccc gcgctgcgcg cctgcctcga ggcgctgtgc cgcgatggcg ctgaacgcgc
     2341 gcgcctgggc gccgcggcgc gcgaacgcgt gctgtccacg ttcgacatcc gccagatgat
     2401 cgagcgctac gagcggctct atgagcgcgt gcacgcgcgc accgtgcgat gagcgacgcc
     2461 cggccgccag caggcgcgcg caccgcaatg cgcagcacgg agcctgcccg atgaacagca
     2521 cgggcggcga gccgctcgtc gccgcggcgg cccgccccgc ggtgccgcgc gccgaggcca
     2581 gccccgccga gggccggctg cgcgtcgtcg tgctcaccac cttcttcccg agcccgcgcg
     2641 acccgttccg cacgccgttc ctgcgcaacc tggtcgacgc gatggccggc gactgcgacc
     2701 aggagctggt gatccccgtg ccgcggcgcc cgccgatcgg accctggcgc gacgcccagc
     2761 ccgtgcccgc cagcgaaacc cacgccggct ggacgctggc gcacccgcgc tacctggcga
     2821 tcccggggct gcactggctg tcagggctca cctacttcct gggcacgtgg cgcacgctgc
     2881 gcgcgctcag gcggcgccac ggccgctacg tcctgcacgc gcactgcgcc taccccgacg
     2941 tggtcggcgc cgcgctcgcc gccaaggtgc tggggctgcc gctggtggcc accgcgcatg
     3001 gcagcgacat caacatcagt gggcgccagt ggctgctgcg gccgcagatc cgctgggcgc
     3061 tgcgcgccgc gcgccgcgtc atcgcggtca gcgcgccgct cgccgcggcc gtctcggagc
     3121 tcacgaaatt gccggcggag ggggttcggt gcattccgtg tgccggctac ttccccgcgg
     3181 tgttccgtcc gcgcgacgcc ggcgagcgtg ctgcactgcg gcaagccctg ggcatcgcgc
     3241 acgatgccca ggtcgtcctt ttcgtgggtc acctggtgcc ggtcaaggcg ctggacgtgt
     3301 tgctgcgcgc gtggtcgacg ttgttgcaat tttcgtcggt aggcgctcac cttcgactgg
     3361 tattgatcgg ggaaggcacg ctgcgtgaca cgctcgagca gcaggcccgc gaactgggcg
     3421 tcgccgaccg ggtggccttc ctcggaccga agccgcagcc ggtcgtcgcg gactggatcg
     3481 ccgcggccga cctcctgacg ctgccgagcc atgccgaggg ctcgcccaac gtcgtcgtcg
     3541 aggcgctggc cagcggcacg ccggtggtcg cgagccgcgt gggcggcgtc cccgacctcg
     3601 tcgacgacgg cgtcaacggg ctgctcgtgc cggcgggcga tgccgcggcg ctggcccagg
     3661 cgctcggcgc ggcgctggcg cgcgaatggg acgcggcgcg gatctcggcg tcgatcgccc
     3721 acctcacctg gccggccctc gcccggcgca accacgacgt gctcgccgcc gtcgccacgg
     3781 agaccgccca tgctggcgct cgctagaacc ctgtccaagg acctgctgca gcaggccatc
     3841 ttccccgacc gcttcctgtg gcgcctgccc aaaccccggc gcgccatcgc gctgacgttc
     3901 gacgacggcc cgcatcccga gcacacgccg gccatgctgg acgtcctcgc gcgcgcgggc
     3961 gtgcgcgcga cgttctttgt cgtgggacgc gaggtggaga agcaccccga ggtcgcgcgg
     4021 cgcatcgtcg ccgagggcca cggcatcggc ggccacagct acgaccatac ggtcatgacg
     4081 acgctggacg acgcggcgct ggtcgacgac ctggcgcact gccggcgcgt gatccgggag
     4141 gccacgggcc tggacaccgg actctttcgt ccgcccaagg gagaagtcag tcttcgctcc
     4201 atccgcacgg cgtgccgcgc gggctacacg ctggtgcatt ggacccggac ctacagcgac
     4261 tatcgccagg acggcgtgga cgcattgacg cggcgcatcg acgccgaacc gccgatcgca
     4321 ggggacgtcc tgctgttcca cgatcacaac ccgtacacgg tccgcgcgct cgactcgaaa
     4381 ttgccgcagt ggcgcgccaa aggactccaa tttgcaccgt tgaaccgtcc tgtcgagcag
     4441 ccgggaggca gctcgaaggc cggaattggt aagaccctgt aacttgaatt tctgccaaga
     4501 ccgtcacgca tttggtcaaa acgcacattc gttgccctaa agcaacgact gtcgagctag
     4561 cacaacccgg gccgccgcgg acgcctccct aacccgttgg cgtgtcaact ttttggcatc
     4621 tctgccctct gaaattctcg cgaagccggg cattcgcaat ccccattttt ggggaacgtt
     4681 cgatgtgctc cctagggtac cccctagttt gaaatcggcg ctcgacatcg agaaatctgt
     4741 cgcgctgcaa tcgcccggcc gtttgtaacg gtgtcgcaaa cgcgccttac cgacctgctt
     4801 cgggctgccg atacgctgtg catacgcaac gtgccccaag gcttccaacg ccctgacgcc
     4861 agtccggcaa cgccatacgg gacctgcacg gtcggtcaat cctcctccga cccgtcgcac
     4921 ggccctccgc accttgcctt tgagtcgcat cgctacgccg caaggcgggc ggaagcctgt
     4981 ccaaaagaag ttgcctggat ctgcacccat gaattcgatg aagttcgccg cgaagtcggc
     5041 cgtccaactc gccgccctcg ccacgttcgc cgcctacggc agcgccgcgc aggcctccac
     5101 cgtgaccctg acggtcaaat caggcctggg tggtgtcgtc cagagcgacc cctacggata
     5161 catcagctgc acgagcgctc cgcagtcgtc cacctgctcg gccaacgtga cggccggcat
     5221 ggcgatcgcg ctgactgaaa cgccgctgaa gggctatgtc ttcgccggct ggagcggtgg
     5281 cacctgctcc ggcaccgccg cgacctgcta cacccgcgtg atggcgaccg agacgatcac
     5341 cgccaacttc aagtcgacgg ccgcggcccc cgcgccggct cccgcccccg ctccggctcc
     5401 ggctcccgcg cctgcccccg ctccggctcc ggctccggct ccggcccccg cgcctgcccc
     5461 ggctcccgcg cctgctcctg ctcctgctcc tgctcccgcg cctgcccccg cgccggcccc
     5521 ggcccctgct ccggtcggcg ccacgaccgc cccggtcgta gacttcgtcg acacgacgtc
     5581 tgccccgacc atcggtggcg agaacggcgc cggcggctac ctgtcgatct tcggctcgaa
     5641 cttcggtgcg gccagcggca tgggcaccac caccaaggtc tacatcggcg gcgtcgaagt
     5701 cgcgaactac cgctacctgg gcaacgccaa ggtcggcgcc aagctcggcc tgcagcagat
     5761 caccgtccag gtcggttcgc tcggcggcgc caaggtcggc gtggcgcaga acatccaggt
     5821 cgtcgtcaac ggcgtgaaca gcaacgtcaa caacaccttc acgccgaccg ccggcaaggt
     5881 gtggttcgtc tcgctgtccg gcaacgactc gaccgcggtg gccaacgaca tcacgcaccc
     5941 gtaccgctac ctgcagaaca tgaacacgct gacgggcgcc tatttcgcga tgggcaccgg
     6001 cgaccaggtc gtgctgcgcg gcggcaactg gtcggacacg aacggcgtgg actccagctg
     6061 gatgaagttc ggcgccagcc agtacgcccg caacggcacc ccctcggcgt acatcagctt
     6121 caccgcctac ccgggcccgg tcaagggcaa cgccatcgag gacgtgcact acacgacccc
     6181 ggccggcacc tccggcggct tccagggccc gtggtcgtcg atcgccggca ccagcggcga
     6241 gtacatcgcg atctccaacc tgcgcatgga ggtcgccggc ggcgccaacc gtgacgcggc
     6301 tccgatcaac ttccagtaca ccggcggcca ctggcgcgcg gtgaacaacg agatcgggcc
     6361 gtgggtcgcc ggttcctcgg ccatcctgaa cgccgcgggc atctcgggcc acggtgacgg
     6421 caacgtgctg ctgggcaacc acatccacaa catcgccggc ctgtccgacc tgcagaacca
     6481 cggcatctac gcggacacga ccgcgcagaa ctgggacgtc ggctacaact gggtgcacga
     6541 catcaccggc ggcagcgcca tccagttcaa cgacaaccag ggcggcgccg gtagctaccc
     6601 gatgccccac ggcggaacct gggccggctt catcggcatc aagatccaca acaactggtt
     6661 cgagaacgcg gccaagtacg gcatcaactt caacgaccag atgtcgacga aggccggtac
     6721 gtacagcggc atgatctggg acaacgtcgt gatcggcacc gcgctgcccc cgctgcgcat
     6781 caactcgacg cagccgaccc agaagctctg gttcgcgtac aacacgatgt acaactgcat
     6841 gacgaccgtc tcgggcagcg gcaacggcta cgtccgcagc gaaggctggt cggcccaggc
     6901 tggcgtgaac aacgtgttct tcgacaacat cttcgcctac ggcccgaaga cggtcagcgg
     6961 cacccagtac ttcgccaacg tcggtggcac cgcggcgagc acgaccacct acaacttcaa
     7021 gcgcaacctg tacttcccga acggccaggc gccttccgcc gcctcgacca tcggtgacat
     7081 cagcgcgctg accgccgacc cgctgttcgt ggccgccacc tccagcaact tccagacgca
     7141 gtcgacgtcg ccggcgcgcc gcgccgccac gcagggcctg ccctcgggca tgagcgtgac
     7201 gacggacttc accggcctgg tgacccgcgg ctcgccgtcc gacatcggcg cctacgcggc
     7261 gaactgacgc accaggtcag agcgtgaagc ggccgcccgg caacgggcgg ccgctttttt
     7321 tgctcacggc tgacacgtct ggacacgtcc atggccgcgc cgcgattgat taccggccgg
     7381 gcacaccgga caatcccgcg gtctgtggca cgcaggggcc ccggcacatg agggtggttg
     7441 ccgagtggca aggacgacaa cgatgaaaga tggccggatg acccagaact ctccacgcaa
     7501 ccgcgcccgc gccatggcga cgctgggcgt gctcttcgcc ctcgccggtt gcggcggcgg
     7561 cgccagcccc cagagcgtca cgccgacgcc ttccccgacc ccctcgccca cgccggcccc
     7621 gacgccggcg cccggcacgc agacgctgac cctcaacaac gtgggctccg gcagtttgac
     7681 gtccaccacg aacggcgcct cgtgcagcac cgccacgtgc agcaccgagc tcgccaccaa
     7741 cacggtggtg gcgctgacgg ccgtgcccgc ggccgactac accttcggtg gctggggtgg
     7801 cggctgcagc ggcaccggca cgacc
//
LOCUS       DATCRO010000741         8233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_852232, whole genome shotgun sequence.
ACCESSION   DATCRO010000741 DATCRO010000000
VERSION     DATCRO010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852232"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VIN75_20080"
     CDS             <1..856
                     /locus_tag="VIN75_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976523.1"
                     /translation="AAPVNVHRVNWLWANPEAFKKAGAKVPTTWDEFFAAGDKLKAAG
                     IIPLAHGGQNWQDFTTFEDVAMGVGGADFYRNALVKLDAAAINSPTMIKVLETYKKIH
                     GYIDKNAPGRDWNLATAMVIKGEAGMQLMGDWAKGEFLAAGKVPGKDFVCSPAPGTAK
                     DYTFNIDSFAMFQLKDPANQKAQKDLAKAIMSPSFQELFNLNKGSIPVRLDVKLDKFD
                     DCAKESNKDFVASSKANTLLPSIAHSMAVPPAAEGAIKDAVSKFWNDDKETAKDAQAE
                     ILKASKTK"
     gene            1133..2011
                     /locus_tag="VIN75_20085"
     CDS             1133..2011
                     /locus_tag="VIN75_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8976524.1"
                     /translation="MSRPKVPSASWLPKLVVAPSFALSFFFIYGLIAWNGYLSLSASH
                     MLPNYDYVGFSNYTDLFDNDRFHVALRNMGIFGVLFIGGSMVLGLLLAILLDQKIRGE
                     GVLRTIYLYPMAISFIVTGTAWKWILNPGLGIEHLVQSWGFTNFTFDWLVDDEHAIYC
                     IVIAGIWQASGFVMALFLAGLRGIDDSIIKAAQIDGASLPRIYWRIVVPSLRPVFFST
                     LMVVSHLAIKSFDLVIALTAGGPGYSTDLPATFMYAMSFSRGQVAMGAASAEIMLATV
                     AAIVVPYLYSELRTKK"
     gene            2008..2874
                     /locus_tag="VIN75_20090"
     CDS             2008..2874
                     /locus_tag="VIN75_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8976525.1"
                     /translation="MNRPPSSLTWHRVVLWTVLVVFAVWFLTPLYVMIVTSLKDMDQI
                     RGGNLMDLPSHPSFASWGKAWNQACTGTDCGGLKPFFMNSVSMVVPAVAISTSLGIVN
                     GYVLSKWRFKGSEVMFAMLLFGVFMPMQVVLLPMSQILGYFGIASSIVGLIVMHVLAG
                     IPSTTLFFRNYYVGLPDELIKAAMLDGAGFWRILWRVVLPLSTPILVVTLIWQFTNIW
                     NDFLYGVVFSGADSKPITVGLNNLANTTSSVKEYNVDMAAALIAALPTLFVYVVAGKY
                     FVRGLTAGAVKG"
     gene            2879..3943
                     /gene="ugpC"
                     /locus_tag="VIN75_20095"
     CDS             2879..3943
                     /gene="ugpC"
                     /locus_tag="VIN75_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8976526.1"
                     /translation="MGALTIANVRKTFGHVEILKGIDIDIDAGEFLILVGPSGCGKST
                     LLNIIAGLELPTDGSVSIGEREVTYEAPKDRDIAMVFQSYALYPNMNVAQNIAFGLEM
                     RKVPKEKREEAVQRVAKMLQITQLLDRKPGQLSGGQRQRVAMGRALARDPKLFLFDEP
                     LSNLDAKLRVEMRAEIKLLHQRTKTTTVYVTHDQVEAMTLGDRIAVMSGGKVEQFGTP
                     EDIYARPATKFVAEFIGSPAMNMIPAVRAGAGIAANGVDLPLTAAQRDALQKNATAKL
                     TYGLRPEALQLSSDGIPGTMHFTEPTGPETYATVDTAVGMLTLRVPGYMTSKVGDAVH
                     VKWAAEASHLFDGDSGKRIG"
     gene            complement(4231..4701)
                     /locus_tag="VIN75_20100"
     CDS             complement(4231..4701)
                     /locus_tag="VIN75_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8976527.1"
                     /translation="MAHVHTALIRWEAGDADFLGKRYSRAHTWTFDGGAVVPGSSSPL
                     VVPLPMSDARAVDPEEAFVAALSSCHMLWFLDLASRAGIAIGSYEDAAEGHMGRNADG
                     KLVVDVVTLRPRTRVTGARVPDAAELAALHHRAHEECFLANSVRCEIRCEPSME"
     gene            complement(4712..5410)
                     /locus_tag="VIN75_20105"
     CDS             complement(4712..5410)
                     /locus_tag="VIN75_20105"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976528.1"
                     /translation="MTTLVNIAIVGDRDDTIAAHRAIPLALAHAASALDVALVFDWLA
                     SDAILDASRLAGYDAFWVAPGSPYRSLDGALRAIGHARTTGRPFLGTCGGFQHAVLEF
                     ARHVLDWPDAMHAESDPGVGRPVIAALECSLVEARGSVTLAPGSRIARAYGGLAAPTI
                     YRCRYGLNPAFRAALTSGPLRGTAEDEHGDLRAVELDGHPFFVATLWQPERAALEGQR
                     VPLAEALVTAARAA"
     gene            complement(5421..6059)
                     /locus_tag="VIN75_20110"
     CDS             complement(5421..6059)
                     /locus_tag="VIN75_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8976529.1"
                     /translation="MAGTQHLWLFIVSGLLLNITPGPDSLFIMARSASQGWRAGVVAC
                     WGVGSGVFVHVFAAALGLSALLATSAMAFAAVKIVGAAYLVWIGIGMLRARRAAGAAP
                     AVDVAPRAIPYRQIFRQGFLTNVLNPKVALFFLAFVPQFIAPDAASKPLAFVLLGAIF
                     DFNGMLWCHALALFTAFASRQLQVGAAVARWLNRAIGAMFLALGVRLALATR"
     gene            6167..7060
                     /locus_tag="VIN75_20115"
     CDS             6167..7060
                     /locus_tag="VIN75_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976530.1"
                     /translation="MDKLRAMKTFVQIADDGSLTAAAAALGMSLPAVVRSLAALEADL
                     GARLFQRTTRRLALTQEGLQYLARSRDILAAVAEADASLADETQAPRGQLAITAPVLL
                     GQRVVADFVTRFVAANPRIRCSVQLLDRFVDLVDEGVDVGIRIGALEDSSLVAIRLGE
                     VRQMVVASPAFLRRNGTPKHPTELRGRPCVRLAGPLRPGWAFQDGGRRLHVPVDGPLD
                     FNHAGAALLACEQGAGFGQFFSYQVQDALHERRLRVVLAEHEPPRQPVSIVYPHARLL
                     PARTRAFIDAARRELGPMFQG"
     gene            complement(7051..>8233)
                     /locus_tag="VIN75_20120"
     CDS             complement(7051..>8233)
                     /locus_tag="VIN75_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8976531.1"
                     /translation="FGGKPLEWERSGGKVVGVYTPNWQPAALVDALHGDSVTHVVYAF
                     LHVCGPGQLPEDAPKCVGKADYQIATSAIDDTFNDAFARLKARAPHVKVVASVGGWGG
                     SDPFFHIADDAAPRAAFAKSAADFLRTHPAFDGIDIDWEHPTNNGSANGVQLGSPADG
                     QGVADLMAALRGAMDTLTGENGHKYILTAAINCEHAIVDKVNYRDAAKSMDYIFMMTY
                     DFYGSWKKVIGNHAAPRASAAGAEDGLDVAIRTMTGAGVPAGKLVAGVAMYGRGFTGV
                     ARPAPGHGFTGEAHTGTYPAPEGASDWRELAARYVDRHGRGLHGYQAVFDPQTHAWNL
                     YNAHNKLFMGYDDPRAVIEKGHMAREAGLAGVFAWELSQDNGDILNAMNYGVGNQLLQ
                     P"
BASE COUNT         1272 a         2854 c         2837 g         1270 t
ORIGIN      
        1 cgccgcgccg gtcaacgtgc accgcgtgaa ctggctgtgg gccaacccgg aagcgttcaa
       61 gaaggccggc gccaaggtcc cgacgacctg ggacgagttc ttcgccgccg gtgacaagct
      121 caaggccgcg ggcatcatcc ccctggccca cggcggccag aactggcagg acttcacgac
      181 gttcgaagac gtcgcgatgg gcgtcggcgg cgccgacttc taccgcaacg cgctggtcaa
      241 gctcgatgcg gccgcgatca acagcccgac catgatcaag gtgctggaga cgtacaagaa
      301 gatccacggc tacatcgaca agaacgcccc gggccgcgac tggaacctgg ccaccgcgat
      361 ggtcatcaag ggcgaggccg gcatgcagct gatgggcgac tgggccaagg gcgagttcct
      421 cgccgccggc aaggtcccgg gcaaggactt cgtctgctcg ccggccccgg gcacggccaa
      481 ggactacacc ttcaacatcg actcgttcgc gatgttccag ctgaaggatc cggccaacca
      541 gaaggcgcag aaggacctgg ccaaggcgat catgtcgccg tcgttccagg agctgttcaa
      601 cctgaacaag ggctcgatcc cggttcgcct cgacgtcaag ctcgacaagt tcgacgactg
      661 cgccaaggaa tcgaacaagg acttcgtggc ctcgtccaag gccaacacgc tgctgccgtc
      721 gatcgcccac agcatggccg tcccgccggc cgccgaaggc gccatcaagg acgccgtcag
      781 caagttctgg aacgacgaca aggaaacggc caaggatgcc caggccgaga tcctgaaggc
      841 ttccaagacg aagtgatcga cgaggcggcc gccccgcggc ggccgccttc ttctcctccc
      901 aagggcattc gccacgcgga ttgcgcgcgt ggcgcttttt ttgcacttgc tggcttttgc
      961 actcagaatc cgtgagcgaa cgcggcccgc gccgggctcg ctcacggatt ctcgggctcc
     1021 ccgggctgcg gtccccggcc agcctccagc cttctcgggt tcggcccttg ccggcagcaa
     1081 cgctgccgtc cacgccccgc cccgaccgcg taacgagagc ctcgtcgctt aaatgtcccg
     1141 acccaaagtc cccagcgcct cctggctgcc gaagctggtc gtcgcgccca gcttcgcgtt
     1201 gtcgttcttc ttcatctacg gcctgatcgc gtggaacggc tacctgtcgc tgtccgcgtc
     1261 gcacatgctg ccgaactacg actacgtcgg cttctccaac tacaccgacc tgttcgacaa
     1321 cgaccgcttc cacgtggcgc tgaggaacat gggcatcttc ggcgtgctgt tcatcggcgg
     1381 ctcgatggtg ctgggcctgc tgctggccat cctgctggac cagaagatcc gcggcgaggg
     1441 cgtgctgcgc acgatctacc tgtacccgat ggcgatctcg ttcatcgtca ccggcacggc
     1501 ctggaagtgg atcctgaacc cgggcctggg catcgagcac ctggtgcaga gctggggctt
     1561 cacgaacttc accttcgact ggctggtgga tgacgagcac gcgatctact gcatcgtgat
     1621 cgccggcatc tggcaggcct cgggcttcgt gatggcgctg ttcctggccg gcctgcgcgg
     1681 catcgacgac tccatcatca aggccgcgca gatcgacggc gcgagcctgc cgcgcatcta
     1741 ctggcgcatc gtcgtgccgt cgctgcggcc cgtgttcttc agcacgctga tggtggtgag
     1801 ccacctggcg atcaagagct tcgacctcgt gatcgcgctg accgccggcg gcccgggcta
     1861 ctcgaccgac ctgcccgcca ccttcatgta cgcgatgtcg ttctcgcgcg gccaggtcgc
     1921 catgggcgcc gcgagcgccg agatcatgct ggccaccgtg gccgccatcg tcgtgcccta
     1981 cctgtattcc gaactgagga ccaagaagtg aaccggccgc cgagctccct cacctggcac
     2041 cgcgtcgtcc tgtggacggt gctggtcgtc ttcgcggtct ggttcctgac tccgctgtac
     2101 gtgatgatcg tcacgtcgct caaggacatg gaccagatcc gcggcggcaa cctgatggac
     2161 ctgccgtcgc acccgagctt cgcctcctgg ggcaaggcct ggaaccaggc ctgcacgggc
     2221 accgactgcg gcggcctgaa gccgttcttc atgaacagcg tgtcgatggt ggtgccggcg
     2281 gtggcgatct ccacctcgct gggcatcgtc aacggctacg tgctgtccaa gtggcgcttc
     2341 aagggcagcg aggtgatgtt cgcgatgctg ctgttcggcg tgttcatgcc gatgcaggtg
     2401 gtgctgctgc cgatgtcgca gatcctgggc tacttcggca tcgccagctc catcgtgggc
     2461 cttatcgtga tgcacgtgct cgccggcatc ccgtcgacca cgctgttctt ccgcaactac
     2521 tacgtcggcc tgcccgacga gctgatcaag gccgccatgc tggacggcgc cggcttctgg
     2581 cgcatcctgt ggcgcgtggt gctgccgctg tcgacgccca tcctggtcgt cacgctgatc
     2641 tggcagttca ccaacatctg gaacgacttc ctttacgggg tggtcttctc gggcgcggat
     2701 tccaagccga tcaccgtcgg cctgaacaac ctcgcgaaca cgaccagttc ggtcaaggaa
     2761 tacaacgtgg acatggccgc ggcgctcatc gctgcgctgc cgacgctgtt cgtctacgtg
     2821 gttgctggca agtatttcgt gcgcggcctg acggccggcg cggtgaaggg atgatgagat
     2881 gggtgcgctg acgatcgcca acgtacgcaa gactttcgga cacgtcgaga tcctcaaggg
     2941 catcgacatc gatatcgacg cgggtgaatt cctgatcctg gtgggcccct cgggctgcgg
     3001 caagtccacg ctgctgaaca tcatcgcggg cctggagctg cccaccgacg gttcggtcag
     3061 catcggcgag cgcgaggtca cctacgaggc gccgaaggac cgcgacatcg cgatggtgtt
     3121 ccagagctac gcgctctatc cgaacatgaa cgtggcgcag aacatcgcgt tcggcctcga
     3181 gatgcgcaag gtgcccaagg aaaagcgcga ggaggccgtg cagcgcgtgg ccaagatgct
     3241 gcagatcacg cagctgctcg atcgcaagcc gggccagctc tcgggcggcc aacggcagcg
     3301 cgtggccatg ggccgggcgc tggcgcgcga cccgaagctg ttcctgttcg acgagccgct
     3361 gtccaacctc gacgccaagc tgcgcgtgga gatgcgcgcc gagatcaagc tgctgcacca
     3421 gcgcacgaag acgacgacgg tctacgtcac ccacgaccag gtcgaggcca tgacgctggg
     3481 cgaccgcatc gccgtgatga gcggcggcaa ggtggagcag ttcggcacgc ccgaggacat
     3541 ctacgcgcgc ccggccacca agttcgtcgc ggagttcatc ggctcgcccg cgatgaacat
     3601 gatcccggcc gtgcgcgccg gcgcgggcat cgccgccaac ggcgtcgacc tgccgctgac
     3661 ggccgcgcaa cgcgacgcgc tgcagaagaa cgccaccgcc aagctcacct acggcctgcg
     3721 cccggaggcg ctgcagctgt cgtccgacgg catccccggc acgatgcact tcaccgagcc
     3781 gacgggcccc gagacctacg ccaccgtcga cacggccgtg ggcatgctga cgctgcgcgt
     3841 gccgggctac atgacgtcca aggtgggcga cgccgtgcac gtgaagtggg ccgccgaggc
     3901 gtcgcacctg ttcgacgggg acagcggaaa gcgtatcggc tagagcgccg cccgctcgcg
     3961 aagacgaggg cgcgatgcga atcgcgccct tttcgtttcc gcgcacgcct gctcgcgcaa
     4021 gggtctggcc tctcctcggc atcgcgccat tttcggctcg tcagctgtga ggacgtccgc
     4081 gatgtcgagg cgaaggcctg acctcgatcg atggtgggga tcggggtcag gccttcgcct
     4141 cgcgtcgccg acggcatcgt cgcggctggc caggacgcag cggcgacgtg aggagaggcc
     4201 agacccctcc ggcatcgggg ggcccgcagg tcattccatc gacggctcgc agcggatctc
     4261 gcagcgcacc gagttcgcga gaaagcattc ctcgtgcgcc cggtgatgca gggccgccag
     4321 ttccgcggcg tcgggcacgc gcgcgcccgt cacgcgcgtg cgcgggcgca gggtcacgac
     4381 gtccaccacc agcttgccgt cggcgttgcg gcccatgtgc ccttcggcgg cgtcctcgta
     4441 gctgccgatg gcgatgccgg cgcgcgacgc gaggtcgagg aaccacagca tgtggcagct
     4501 ggagagcgcg gcgacgaagg cctcctccgg gtcgacggcg cgggcgtcgg acatcggcag
     4561 cggcaccacc agcggcgacg acgagccggg cacgacggcg ccgccgtcga aggtccaggt
     4621 gtgggcgcgg ctgtagcgct tgcccaggaa gtcggcgtcg ccggcttccc agcggatcag
     4681 ggcggtgtgg acatgggcca tggtcgggct ttcaggcggc gcgcgccgcg gtgacgaggg
     4741 cttcggcgag cggcacgcgc tggccttcga gcgccgcgcg ctcgggctgc cacagcgtcg
     4801 cgacgaagaa cgggtggccg tcgagctcga cggcacgcag gtcgccgtgc tcgtcctcgg
     4861 cagtgccacg cagcgggccg gacgtcaacg cggcgcggaa cgccgggttc agtccgtagc
     4921 ggcaccggta gatcgtcggc gcggccagtc cgccgtaggc tcgcgcgatg cggctgccag
     4981 gcgcgagcgt gacgctgccg cgcgcttcca ccagcgagca ctccagcgcc gcgatgaccg
     5041 gccgcccgac acccgggtcg ctctcggcat gcatcgcgtc gggccagtcc agcacgtggc
     5101 gcgcgaactc gagcaccgcg tgctggaagc cgccgcaggt gcccaggaac ggccggccgg
     5161 tggtgcgggc gtgtccgatg gcgcgcagcg cgccgtcgag gctgcggtac gggctgccgg
     5221 gcgcgaccca gaaggcgtcg tagcccgcga ggcggctggc gtcgaggatc gcgtcgctgg
     5281 ccagccagtc gaacacgaga gccacgtcca gggccgaggc cgcgtgggcc agcgccagcg
     5341 ggatcgcgcg gtgcgcggcg atcgtgtcgt cgcggtcgcc gacgatggcg atgttcacga
     5401 gggtggtcat catgccgcct tcagcgcgtc gccagcgcca gccgcacgcc gagcgccagg
     5461 aacatcgcgc cgatcgcccg gttcagccag cgcgcgacgg ccgcacccac ctgcagctgg
     5521 cggctggcga acgccgtgaa cagcgcgagg gcgtggcacc acagcatgcc gttgaagtcg
     5581 aagatcgcgc ccagcagcac gaacgcgagc ggcttggacg ccgcgtccgg ggcgatgaac
     5641 tgcggcacga acgcgaggaa gaacagcgcc accttcggat tgagcacgtt ggtcaggaag
     5701 ccctggcgga agatctggcg atacgggatc gcgcggggcg cgacatcgac ggcaggcgcc
     5761 gcgccggcgg ccctgcgcgc ccgcagcatg ccgatgccga tccacacgag gtacgccgcg
     5821 ccgacgatct tgacggccgc gaacgccatc gccgacgtcg ccagcagcgc cgacagcccc
     5881 agcgccgccg cgaacacgtg cacgaacacg cccgatccga caccccagca cgccacgacg
     5941 ccggcccgcc agccctgcga cgcgctgcgc gccatgatga acagcgagtc gggccccggg
     6001 gtgatgttga gcagcagccc cgagacgatg aacaaccaaa gatgctgggt tccggccatg
     6061 cggccctccg tgtcgacgtg gggaccggat catcgccgcg tcatgcgacg cagtaaacgc
     6121 cgctggtcgc agaggtttgt tgcgcatggc ggattaatat ggcgcgatgg acaagctccg
     6181 cgccatgaag accttcgtgc agatcgccga cgacggcagc ctcaccgccg cggcggcggc
     6241 gctgggcatg tcgctgcccg ccgtcgtgcg cagcctcgcg gcgctggagg ccgatctggg
     6301 cgcacgcctg ttccagcgca ccacgcggcg cctggcgttg acgcaggagg gcctgcagta
     6361 cctggcgcgc agccgcgaca tcctcgccgc cgtcgccgag gccgacgcga gcctcgccga
     6421 cgagacgcag gcgccgcgcg gccagctcgc gatcaccgcg ccggtgctgc tgggccagcg
     6481 cgtggtggcc gacttcgtca cccgcttcgt cgccgccaac ccgcgcatcc gctgcagcgt
     6541 gcagctgctc gatcgcttcg tcgacctggt cgacgaaggg gtcgacgtgg gcatccgcat
     6601 cggcgcgctg gaggactcgt cgctggtggc gatccgcctc ggcgaggtgc ggcagatggt
     6661 cgtggcgagc ccggcgttcc tgcgccgcaa cggcacgccg aagcatccga cggagctgcg
     6721 cggtcggccc tgcgtgcggc tcgcgggacc gctccgcccg ggctgggcct tccaggacgg
     6781 cgggcgccgg ctgcacgtgc cggtcgacgg gccgctcgac ttcaaccacg ccggcgccgc
     6841 gctgctggcc tgcgagcagg gcgcgggctt cggccagttc ttctcgtacc aggtgcagga
     6901 cgcgctgcac gagcgccgcc tgcgcgtggt gctggccgag cacgagccgc cgcgccagcc
     6961 ggtgagcatc gtctatccgc acgcccggct gctgccggcg cgcacgcggg ccttcatcga
     7021 cgcggccagg cgcgagctag ggccgatgtt tcaaggctga aggagctggt tgcccacgcc
     7081 gtagttcatc gcattgagga tgtcgccgtt gtcctgcgac agctcccagg cgaacacccc
     7141 ggccagaccg gcctcgcgcg ccatgtggcc cttctcgatc actgcccgcg ggtcgtcgta
     7201 gcccatgaag agcttgttgt gcgcgttgta gaggttccag gcgtgcgtct gcggatcgaa
     7261 gaccgcctgg tagccgtgca gcccccggcc gtggcggtcg acgtagcgcg ctgcgagctc
     7321 gcgccagtcg ctggcgcctt cgggcgccgg ataggtgccg gtgtgcgcct cgccggtgaa
     7381 gccgtggccg ggggccggtc tggcgacgcc cgtgaagccg cggccataca tcgccacgcc
     7441 cgccaccagc ttgccggcgg gaacgcccgc gccggtcatc gtgcggatcg cgacgtcgag
     7501 gccgtcctcc gcgccagcgg cgctggcgcg cggcgcggcg tggttgccga tcaccttctt
     7561 ccagctgccg tagaagtcgt aggtcatcat gaagatgtag tccatcgact tcgcggcgtc
     7621 gcggtagttc accttgtcga cgattgcgtg ctcgcagttg atcgcggcgg tgaggatgta
     7681 cttgtggccg ttctcgccgg tgagcgtgtc catggcgccg cgcagcgcgg ccatcaggtc
     7741 ggccacgccc tggccgtcgg ccggcgagcc cagctgcacg ccgttggccg agccgttgtt
     7801 ggtcgggtgc tcccagtcga tgtcgatgcc gtcgaaggcc ggatgcgtgc gcaggaagtc
     7861 ggccgcggac ttcgcgaacg cggcccgcgg cgcggcgtcg tcggcgatgt ggaagaacgg
     7921 gtcggaaccg ccccagccgc cgaccgaggc caccaccttc acgtgcggcg cgcgcgcctt
     7981 gaggcgggcg aaggcgtcgt tgaaggtgtc gtcgatcgcc gaggtggcga tctggtagtc
     8041 cgccttgccc acgcacttgg gcgcgtcctc gggcagttgg cccgggccac agacgtgcag
     8101 gaacgcgtag acgacgtgcg tgacgctgtc gccgtgcagc gcgtcgacca gcgccgccgg
     8161 ctgccagttg ggcgtgtaga cgccgaccac cttgccgccg ctgcgctccc actccagcgg
     8221 cttgccgccg aac
//
LOCUS       DATCRO010000742         8190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1009620, whole genome shotgun sequence.
ACCESSION   DATCRO010000742 DATCRO010000000
VERSION     DATCRO010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009620"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3940
                     /locus_tag="VIN75_20125"
     CDS             <1..3940
                     /locus_tag="VIN75_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY8976532.1"
                     /translation="TGAYLGARKQVGMGIRRAVVDSTPRLVLQGAREHNLRGVDVEFP
                     LQRLVVVTGVSGSGKSTLMQDVLFPALERHFGKPTETPGAHDRLLGADHLADAVFVDQ
                     SPIGKTARSNPASYVGAFDEIRKLFADSPVARERNYGAGMFSFNAGDGRCPTCGGSGF
                     EHVEMQFLSDVYLRCPDCDGKRFRAEMLDARLVRRDRQLSIADVLELTVSEAVQLFVD
                     DREVIRRLQPIVDVGLDYVKLGQPVPTLSGGEAQRLKLAGFLAEAAQGPQQPAARKGT
                     LFLFDEPTTGLHFDDIAKLMRSLRRLLDAGHSLIVIEHNLDVIRSADWLVDLGPEGGE
                     AGGELVGVGTPEDLKANPRSHTGRALRDYEETLGLERYAPAQAAREAREKHARFAAAE
                     VERDTTRDGPSLQSLVSRRRRASGAVNDEAIRIVNAREHNLKSVDVDIPRGKFTVVTG
                     VSGSGKSTLAFDILFNEGQRRYLESLNAYARSIVQPAGRPEVDAVYGIPPTVAIEQRL
                     SRGGRKSTVATTTEVWHFLRLLWVKLGIQHCVNDGSPVRPQSVESIAAQLLRDYRGRH
                     VGLLAPLVVARKGVYTDLAKWAKARGHTHLRVDGEFLKVDPWPRLDRFKEHTLELPVG
                     DLVIAPEREAELRAMLAKALDIGKGVMHLLAPLDGLTEAMELGHSTLGIGTVKVFSTK
                     RACPTCGTSYGEPDPRMFSYNSKHGWCPHCVGTGLALTREQRAQFDDSVQDDNERGRE
                     QAFITKEPDAEELEDAACPECHGARLAPAPRAIKFEDRAITDVAQWAVTDVRQWVETL
                     RLSGRDADIARDVISEIRSRLVFLEDVGLNYLTLDRAAPTLSGGEAQRIRLAAQLGSN
                     LQGVCYVLDEPTIGLHPRDNRILLDALARLGSQGNTLVVVEHDEDTIRRADHLIDIGP
                     GAGKRGGQLVAAGTAADLSANPASVTGRALAHPMTHPMQPRREVAFAADDAGASTHVT
                     IFGAKLHNLQGMPVRVPLQRLVAITGVSGSGKSTIARDVLLASVAALVSSRGKSAPVG
                     CESVEGWHSVDRVLEVDQTPIGKTPRSCPATYIGFWDAIRKLFAETLEAKARGYSAGR
                     FSFNTGGANGGRCPGCEGQGVRTIEMSFLPDVKVPCDLCHGARFNPATLAVTWRGKSI
                     GDVLQMEVDEAVEFFASMPSIAHPLQLLQDVGLGYLTLGQPSPTLSGGEAQRIKLVTE
                     LSKVRDEVGRRGQKAPHTLYVLDEPTVGLHMADVARLIKVLHRLTDGGHSVVVIEHDL
                     DVIAEADWVIDMGPEGGTGGGRLVAEGPPEHLVAKRTHTGLALAPVLARK"
     gene            4216..4623
                     /locus_tag="VIN75_20130"
     CDS             4216..4623
                     /locus_tag="VIN75_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976533.1"
                     /translation="MPTILTHAAVPPAIGLGLGRRVVFRRLLAVGILPSKSSKSSNFR
                     WRPGAMKHAGPGALDALEDLLVQLRAIDGMHEKSRGVFHLRSKPFLHFHEDPSGLYAD
                     LRAGADFERFKVDALAQQQALVQSVRERLGLGR"
     gene            5047..5604
                     /locus_tag="VIN75_20135"
     CDS             5047..5604
                     /locus_tag="VIN75_20135"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HEY8976534.1"
                     /translation="MTATDERIVELSKAKLAKLLVGACGFVVLGVLMLRPEPEWSRLP
                     FPFDDSTLVRGTGLLAVGFFGLCALFGVRKLFERKPGLVLDSTGILDNTSGIAAGLVP
                     WPEVVGFDIFQLHGARSLVVKVTDPDKYANRGGAIRRALNKANVVLCGSPIAITSSSL
                     KIDFDELLRLCSDYLEKYGDPGARS"
     gene            5825..6259
                     /locus_tag="VIN75_20140"
     CDS             5825..6259
                     /locus_tag="VIN75_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976535.1"
                     /translation="MNIIKTLIALSLAGGAYAAWKHHHQHAARQDAANLSIGAPGAFV
                     PLPPVDGQRPRTVFVIAAEDCPHEDAQRADRLAESLSSQGIPVERTHSANFRFTSQPD
                     SDTVDRISAVMNGPLPVVFIDGRAKANPSLDEVASEFRRSAP"
     gene            6709..7077
                     /locus_tag="VIN75_20145"
     CDS             6709..7077
                     /locus_tag="VIN75_20145"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HEY8976536.1"
                     /translation="MGFHPANLAIRFLLELFALAAMGVWGWQQGGDPARYAFALLVPL
                     VAAAAWGVLAVPGDRSRSGKAPVAVRGAVRLGVEAVFFAFAILALYQSGSKALSAVLA
                     VAVSVHYVASYDRVAWLLRA"
     gene            complement(7177..7836)
                     /gene="sctR"
                     /locus_tag="VIN75_20150"
     CDS             complement(7177..7836)
                     /gene="sctR"
                     /locus_tag="VIN75_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEY8976537.1"
                     /translation="MNGGLPDPLTLVALIVAFGLAPFAALMVTCYTKLVIVFGLLRTA
                     LGLQQTPPNMVLNGIAIILSVYIMAPIGMDVSDALRSHTFGQKGEAVSDIMAVADAAK
                     QPLKDFLQKHTHERERQFFMESATSVWPKARAQALRSDDFIVLVPSFTLSELTRAFQI
                     GFVIYMVFVVVDLIVATILLALGMSMISPTTISLPFKLLLFVMLDGWARLIHGLVLSY
                     R"
     gene            complement(7868..8161)
                     /locus_tag="VIN75_20155"
     CDS             complement(7868..8161)
                     /locus_tag="VIN75_20155"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY8976538.1"
                     /translation="MNRPADPPPAAAPHPAELAVDRLDAIEVTLRFEVGDLAINLGEL
                     RNVRAGHVFELGQPLNRGPVRIVAHGNLLGKGTLVAVGDRLGVRVSEFAPGDL"
BASE COUNT         1238 a         2828 c         2860 g         1264 t
ORIGIN      
        1 gaccggcgcc tacctcggtg cgcgcaagca ggtcggcatg ggcatccggc gcgcggtcgt
       61 cgactcgacg ccgcggctcg tgctgcaggg cgcgcgcgag cacaacctgc gcggcgtgga
      121 cgtcgagttc ccgctgcagc ggctcgtcgt cgtcaccggg gtctccggct cgggcaagtc
      181 cacgctgatg caggacgtgc tgtttcccgc gctcgagcgc cacttcggca agcccaccga
      241 gacgcccggc gcgcacgacc gcctgctcgg tgccgaccat ctcgccgatg cggtgttcgt
      301 cgaccagagc cccatcggca agacggcgcg ttccaacccc gcgagctacg tgggcgcctt
      361 cgacgagatc cgcaagctgt tcgccgacag cccggtggcc agggagcgca actacggcgc
      421 cggcatgttc agcttcaacg ccggcgacgg ccgctgcccg acctgcggcg gctcgggctt
      481 cgagcatgtc gagatgcagt tcctgtccga cgtgtacctg cgttgccccg actgcgacgg
      541 caagcgcttc cgcgccgaga tgctggacgc gcgcctggtg cggcgcgacc gccaattgtc
      601 gatcgccgac gtgctcgagc tcaccgtcag cgaggccgtg cagctgttcg tcgacgaccg
      661 cgaggtcatc cgccgcctgc agcccatcgt cgacgtcggc ctcgactacg tgaagctggg
      721 ccagccggtg cccacgctgt cgggcggcga ggcgcagcgc ctgaagctgg ccgggttcct
      781 ggccgaggcc gcgcagggcc cgcagcaacc ggcggccagg aagggcacgc tgttcctgtt
      841 cgacgagccc accacgggcc tgcacttcga cgacatcgcc aagctgatgc gctcgctgcg
      901 ccggctgctc gacgcggggc attcgctgat cgtcatcgag cacaacctcg acgtgatccg
      961 ctcggccgac tggctggtcg atctgggccc cgaaggtggc gaggccggcg gcgagctggt
     1021 gggcgtgggc acgccggagg acctgaaggc caacccgcgc tcgcacaccg ggcgcgccct
     1081 gcgcgactac gaggagacgc tgggcctgga gcgctatgcg ccggcgcagg ccgcacgcga
     1141 ggcgcgcgag aagcacgcgc gctttgccgc ggccgaggtc gagcgcgaca cgacgcgcga
     1201 cggcccgagc ctgcagtcgc tggtgagccg ccgccgtcgc gcctcgggcg cggtgaacga
     1261 cgaggcgatc cgcatcgtca atgcgcgcga acacaacctg aagtcggtcg atgtcgacat
     1321 cccgcgcggc aagttcacgg tggtgacggg cgtgtcgggc tcgggcaagt cgacgctcgc
     1381 gttcgacatc ctgttcaacg agggccagcg gcgctacctc gagtcgctga atgcctatgc
     1441 gcgcagcatc gtgcagcccg ccggccggcc ggaggtggat gcggtgtacg gcattccgcc
     1501 gaccgtggcc atcgagcaga ggctgtcgcg cggcggtcgc aagagcacgg tggcgacgac
     1561 gaccgaggtc tggcatttcc tgcgcctgct gtgggtcaag ctgggcatcc agcactgcgt
     1621 caacgacggc agccccgtgc ggccgcagag cgtcgagagc atcgccgcac agctgctgcg
     1681 cgactaccgc ggccggcacg tgggcctgct cgcgccgctg gtcgtggcgc gcaagggcgt
     1741 ctacaccgac ctcgccaagt gggccaaggc ccgcggccac acgcacctgc gcgtggacgg
     1801 cgaattcctg aaggttgacc cgtggccgcg cctggatcgc ttcaaggagc acacgctgga
     1861 gctgccggtg ggcgacctgg tcatcgcgcc ggagcgcgag gccgagctgc gcgcgatgct
     1921 ggccaaggcg ctggacatcg gcaagggcgt gatgcacctg ctcgcgccgc tggatggcct
     1981 gaccgaggcc atggagctgg gccattcgac gctgggcatc ggcacggtga aggtgttctc
     2041 caccaagcgc gcgtgcccga cctgcggcac cagctacggc gagcccgacc cgcgcatgtt
     2101 ctcgtacaac agcaagcacg ggtggtgccc gcactgcgtc ggcacgggcc tggcgctgac
     2161 gcgcgagcag cgcgcgcagt tcgacgattc cgtgcaggac gacaacgagc gtggccgcga
     2221 gcaggccttc atcacgaagg agcccgacgc cgaggagctg gaggacgccg cctgccccga
     2281 gtgccacggc gcccgcctgg cgcccgcgcc gcgcgcgatc aagttcgagg accgcgcgat
     2341 caccgacgtc gcccagtggg ccgtgaccga cgtgcgccag tgggtcgaga ccctgcggct
     2401 gtcgggccgc gacgccgaca tcgcgcgcga cgtcatcagc gagatccgca gccgcctcgt
     2461 cttcctggag gacgtgggcc tgaactacct gacgctggac cgcgcggcgc ccacgctctc
     2521 gggcggcgag gcgcagcgca tccgccttgc ggcgcagctg ggctcgaacc tgcagggcgt
     2581 gtgctacgtg ctggacgagc ccacgatcgg cctgcacccg cgcgacaacc gcatcctgct
     2641 ggacgcgctc gcgcgcctgg gctcgcaggg caacacgctg gtggtggtgg agcacgacga
     2701 ggacaccatc cgccgcgccg accacctgat cgacatcggc cccggcgccg gcaagcgcgg
     2761 cggccagctg gtggccgcgg gcacggccgc cgacctctcg gccaacccag cgtcggtgac
     2821 gggccgcgcg ctcgcgcacc cgatgacgca cccgatgcag ccgcgccgcg aggtggcgtt
     2881 cgcggccgac gacgcgggtg ccagcacgca cgtcacgatc ttcggcgcga agctgcacaa
     2941 cctgcagggc atgccggtgc gcgtgccgct gcagcgcctc gtggccatca cgggcgtcag
     3001 cggctcgggc aagagcacga tcgcgcgcga cgtgctgctg gccagcgtgg cggccctggt
     3061 gtcgagccgc ggcaagtcgg cgccggtggg ctgcgagtcc gtcgagggct ggcattcggt
     3121 cgaccgcgtg ctggaggtcg accagacgcc gatcggcaag acgccgcgct cctgcccggc
     3181 cacctacatc ggcttctggg acgcgatccg caagctgttc gccgagacgc tggaggccaa
     3241 ggcgcgtggc tactcggccg gccgcttctc gttcaacacc ggcggtgcga acggcggccg
     3301 ctgcccgggc tgcgaaggcc agggcgtgcg cacgatcgag atgagcttcc tgcccgacgt
     3361 gaaggtgccg tgcgacctgt gccatggcgc gcgcttcaac ccggccacgc tggcggtcac
     3421 gtggcgcggc aagtcgatcg gcgacgtgct gcagatggag gtcgatgagg cggtggagtt
     3481 cttcgccagc atgccgtcca tcgcgcatcc gctgcaactg ctgcaggacg tcggcctggg
     3541 ctacctgacg ctgggccagc cgtcgccgac gctctcgggc ggcgaggcgc agcgcatcaa
     3601 gctggtgacc gagctgagca aggtgcgcga cgaggtcggc cgccgcggcc agaaggcgcc
     3661 gcacacgctg tacgtgctgg acgaaccgac ggtgggcctg cacatggccg acgtcgcgcg
     3721 cctgatcaag gtgctgcacc gcctcaccga cggcggccac agcgtggtgg tgatcgagca
     3781 cgacctcgac gtcatcgccg aggccgactg ggtcatcgac atgggtcccg aaggcggcac
     3841 cggcggcggc cggctggtgg cggaaggtcc gccggagcac ctggtggcca agcgcaccca
     3901 cacgggcctc gcgctggcgc ccgtgctcgc gcgcaagtga cgccccgcgc gccgcccgcg
     3961 cgcgaacgcg gcggcgcgtg acacccgcgc gcgctggcgt atgcccgcaa gtcgcgcgtg
     4021 cggcgcgcgg ggtggcgatg agcggggtgg acggcatgcg atgctcgatg gacgtgaggc
     4081 catcgtaggc cggcgcacga tttctgcccg gcgccaacgg gccggtttcc gcggtttcgg
     4141 cggggatcgg gccggcagcg gccggctgcg accggccgcc gcgtacagcc accatcgagt
     4201 acgctgtggg gcgcgatgcc gaccatcctc actcatgcgg ccgttccacc ggcgatcggt
     4261 ctcgggctgg gccgacgtgt cgtgttcagg cgcctgctcg ccgttggcat cctgccgtcg
     4321 aagtcgtcga agtcgtcgaa tttccgctgg agaccagggg ccatgaagca tgccggaccg
     4381 ggtgcgttgg atgcactgga agacctgctg gtacaactgc gcgccatcga cggcatgcac
     4441 gagaaatcgc gcggtgtgtt ccacctgcgc tccaagccct tcctgcactt ccatgaagat
     4501 ccatcgggcc tctatgcgga cctccgggcg ggcgcggact tcgagcgttt caaggtggat
     4561 gcgcttgcgc agcagcaggc cctggtgcaa tcggtccgtg agcggctggg gctcggtcgt
     4621 tgaacgcaga cgagccgcgc ttggcgggtg gaccgcccgt cgcgacctcg gcgcccgtgg
     4681 gtcgcgtttc ggccgggaac ggaggaagct cgacgaccgg cttcagggag ttcgactgac
     4741 catgcctgca ccgcgatgat cgttggatcc ggccgtgctc aaatccccgg ccaaagccgt
     4801 agacgcccga cgcaatgcac gccctgcctc catcgactcc ttcgccgctc ctgaccgctg
     4861 caagtcttct gctggcggca agccttggaa ccgcgaatgc tcaattctct gaccctcatc
     4921 cggccgtcgg acaagtgggc ggatcgcgcg gcacccgtcc tgcggccgga gccggtcgtc
     4981 agcgacaggg cttccctgcc gtcacaatga cttggcgtga gctgcaaatc tgcccagggg
     5041 aaccgcatga ccgccactga tgaacgcatc gtcgaactga gcaaggccaa gcttgccaag
     5101 ctcctcgtcg gcgcctgcgg gttcgtcgtt ctcggcgtcc tgatgctccg gcccgagccc
     5161 gaatggagca gattgccgtt tcccttcgat gattcgacgc tcgttcgtgg aacaggtctg
     5221 ttggccgtcg gcttctttgg actgtgtgcg ctcttcggag tcaggaagct cttcgaacgg
     5281 aagccgggcc tggtcctgga ctcgaccggc attctcgaca acaccagcgg tatcgctgcg
     5341 ggccttgttc cctggcctga agtcgtgggt ttcgacatct tccagctgca tggcgcaagg
     5401 tcactcgtgg tcaaggtgac cgacccggac aagtatgcca atcgcggtgg cgcgatccgg
     5461 cgcgcgctga acaaggccaa cgtcgtcctg tgcggcagcc cgatcgccat cacgtcgagc
     5521 tccctcaaga tcgacttcga tgagcttctt cgactttgca gcgactatct tgaaaagtac
     5581 ggcgatcctg gggcgcgctc atgacgtctc ggcagcatgt cgtgctggtc ggatccatcg
     5641 tccgatgggg cagccgcgtt acgatgtgcc cgagcgcttt gaagtgctgc cgtctacgaa
     5701 tgtctccgtg cgggcgggcg cgcgcccgta gcgagatgcg gcgatcggca gtgcagttcc
     5761 ggcggcccgc aggaatccat cagggcacac ttgactgaca attccacatc gggaccgcgc
     5821 cgacatgaac atcatcaaga ccttgatcgc gttgagcctc gccggaggcg catacgcggc
     5881 ctggaagcac catcaccagc acgcggcacg ccaggatgcc gcgaacctgt cgatcggcgc
     5941 tccgggtgcg ttcgttcccc tgccgcccgt cgacgggcag cggccgcgga ccgtattcgt
     6001 catcgcggcc gaggactgcc cgcacgaaga cgcccagcga gctgaccgcc tcgccgagtc
     6061 gctgtccagc cagggaattc cggtcgaacg cacgcactcg gccaatttcc gcttcaccag
     6121 ccagcccgac tccgacacgg tcgatcgcat ctccgcggtc atgaacgggc cgctgccggt
     6181 ggtcttcatc gacgggcggg cgaaagccaa cccgtccctg gacgaagtcg cctccgaatt
     6241 caggcggagc gcgccgtagg cctccgcgtc ctggctgggg cgcacgcgac cgactggcga
     6301 cggccaggag cgacgctctg gtcgcgaccg cttcaggcaa tcgagctcgc gacgacgcgg
     6361 gacgctttcg ccgcccgatg gagcgccgcc cccggatgaa tggccggcga tgcctgggag
     6421 ccgtcgctcg acgacggaat tcggccgtcc gtcgcaatgt ctcgttcgct cggtcttgag
     6481 cgtctagagt cgagcctgcg ttggcattcg caagggactc tccatggcgt tcaagtcggc
     6541 aatcggggcg atgctgctcg tcgagaccgt gttcgccatg catgagggcc ctcggccctg
     6601 gaaggacgtg gtcaggctgg tcgggacctc gcccggctga tcgtggcgtg caggacggcg
     6661 ctcgccacga atgacatcga atcgcgccgg ttgcgcgggg aaacgcacat gggcttccat
     6721 ccggccaatc tggccatccg attcctgctc gagctgttcg ctctcgccgc catgggggtc
     6781 tggggctggc agcagggcgg tgatcccgca cgctacgcct tcgcgcttct cgtccccctc
     6841 gtcgcggcgg cagcatgggg cgtccttgcc gttcccgggg atcgaagccg ttccggcaag
     6901 gcacccgtgg ccgtgcgcgg cgcggtccgg ctcggcgtcg aggctgtctt ctttgccttt
     6961 gcgatccttg cgttgtacca gtctggaagc aaggcactct cggcagtcct ggccgtcgcc
     7021 gtttccgttc actacgtcgc ctcgtacgat cgcgtcgcct ggctgctgcg tgcctgacag
     7081 gccacgtgaa tccgcaaggt tcgcggcgcc ttgtgcaacg acccggccgt caaggctgct
     7141 tggctcggcg gcgctcgcgc ggacccttcg cgatgctcag cgatacgaca gcaccagccc
     7201 gtggatcagg cgcgcccagc cgtccagcat cacgaacagc agcagcttga acggcagcga
     7261 gatcgtcgtc ggcgagatca tcgacatgcc cagcgcgagc aggatggtcg ccacgatcag
     7321 gtcgacgacc acgaacacca tgtagatgac gaagccgatc tggaacgcgc gcgtcaactc
     7381 gctgagcgtg aagctgggca ccagcacgat gaagtcgtcc gagcgcagcg cctgcgcgcg
     7441 cgccttgggc cagacgctgg tggccgattc catgaagaac tgccgctcgc gctcgtgggt
     7501 gtgcttctgc aggaagtcct tcagcggctg cttggcggcg tcggccacgg ccatgatgtc
     7561 ggagacggcc tcgcccttct gcccgaaggt gtggctgcgc agcgcgtcgc tgacgtccat
     7621 cccgatgggc gccatgatgt agaccgacag gatgatcgcg atgccgttga gcaccatgtt
     7681 gggcggcgtc tgctgcaggc cgagcgccgt gcgcaacagg ccgaacacga tcaccagctt
     7741 ggtgtaacac gtcaccatca acgcggcgaa cggcgcgagg ccgaacgcga cgatcagggc
     7801 gacgagggtc agcggatcgg gcaggccgcc gttcacgctg ccgccctccg gagcgggcgc
     7861 gcgcggctca caggtcgccc ggcgcgaact cgctgacgcg cacgccgagc cggtcgccga
     7921 ccgccacgag cgtccccttg cccagcaggt tgccgtgcgc gacgatgcgc accggcccgc
     7981 ggttgagcgg ctgcccgagc tcgaacacgt ggccggcgcg cacgttgcgc agctcaccga
     8041 ggttgatcgc gaggtcgccg acctcgaagc gcagcgtcac ctcgatcgcg tcgagccgat
     8101 cgacggcgag ctcggcgggg tgcggcgcgg cggccggcgg cgggtcggcg ggacggttca
     8161 tcgtctgttc cctcgtttgc tggatcgtga
//
LOCUS       DATCRO010000743         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_863744, whole genome shotgun sequence.
ACCESSION   DATCRO010000743 DATCRO010000000
VERSION     DATCRO010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863744"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VIN75_20160"
     CDS             <1..194
                     /locus_tag="VIN75_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY8976539.1"
                     /translation="VGIVSGTWTAFADGVKPVHSIVLGGDKFTVYTGLLALAVNVVVA
                     VVVQALLRSPAPATASGKA"
     gene            264..1649
                     /locus_tag="VIN75_20165"
     CDS             264..1649
                     /locus_tag="VIN75_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8976540.1"
                     /translation="MRLKAKLLVLAAVPLLLSLALIATAVQLQQRELAKREHALVEGG
                     YLSARRAELKNYVQLAMSAIAPMAESHDDVAGSQARAMRLLESMEYGDDGYFFVYDMH
                     GTVLMHPRQRDLVGKDLSGLRDPQGNATIQELLARARAGGGFVEYMWRRPSTGKIAPK
                     LGYVTRIPQWDWMIGTGLYRDDIDSATSQLDAQASSNIGETLLWIAAIAFGGVALISF
                     SGIWLNLSELRVADAKLQTLARTVVRFQEDERAHLARELHDGATQTMVSAKLLVESAI
                     DQLEREARAAPRVLETALAGLKQSLDEVRRISHRLRPAMLDELGLPAALELLTREAED
                     AGATPVHLSVSGDATPLPEEVKTALFRVSQEALANVAKHAHAHRVEMALDFGLAGVAL
                     RIDDDGRGFEVDRIQLDPDRGIGLRNMRERLASIGGTFEVHASPGAGTLLVAKVPADA
                     IRRLAVAGNAA"
     gene            complement(1775..>2824)
                     /locus_tag="VIN75_20170"
     CDS             complement(1775..>2824)
                     /locus_tag="VIN75_20170"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY8976541.1"
                     /translation="PVVFVKGRGAGSNRPSRYVKDEWDAAADVDPAARRTEVTEREAK
                     SILNRNQSPDLDFDLTLNPYQGCEHGCVYCFARPTHAYLNLSPGLDFETRLFAKMNAA
                     QVLRKELSRRGFNPGIVALGAATDAYQPIDRKYALARQVLEVLAEFDVAVGITTKSAL
                     VTRDIDILAPMARKGLVRVHMSIPTLDHELSRRLDPRANSPTRRLEAIEDLTSAGIPV
                     AVYIAPVIPSLNEHEIEAILEACVARGATMGLYTLLRLPREVRDLFAEWLRRWYPERA
                     AQVWRLIHRMHEGKDYDSRFGNRMVGTGSVAEALARRYDEAAARLGVTFPGRTLNTSL
                     FRPRGAEPQAQASLF"
BASE COUNT          396 a          996 c         1017 g          415 t
ORIGIN      
        1 tggtgggcat cgtgtccggc acgtggacgg cgttcgccga cggcgtcaag ccggtgcact
       61 cgatcgtgct gggcggcgac aagttcaccg tctacaccgg cctgctggcg ctggcggtca
      121 acgtggtcgt ggccgtcgtc gtccaggcgc tgctgcgctc gccggcgccg gcgaccgcgt
      181 ccggcaaggc ctgatccgct cgtcggccat cccgggccgc gcgggcgtcg ccgacgtccg
      241 cgcggccttg tcacaatcgc gccatgcgtc tgaaggccaa gctgctcgtc ctcgcggcgg
      301 ttccgctgct gctgtcgctt gccctgatcg ccacggcggt gcagctgcag cagcgcgagc
      361 tcgccaaacg cgagcacgcg ctcgtggagg gcggctacct cagtgcgcgg cgcgcggagc
      421 tcaagaacta cgtgcagctc gcgatgagcg ccatcgcccc gatggccgaa tcgcacgacg
      481 acgtcgccgg ctcgcaggcg cgcgcgatgc ggctgctcga atcgatggag tacggcgacg
      541 acggctactt cttcgtctac gacatgcacg gcaccgtgct gatgcatccg cgccagcgcg
      601 acctcgtcgg caaggacctg tcgggcctgc gcgacccgca gggcaacgcc acgatccagg
      661 agctgctcgc gcgtgcacgc gcgggcggcg gcttcgtcga gtacatgtgg cggcggccat
      721 cgaccggcaa gatcgcgccc aagctcggct acgtcacccg gatcccgcag tgggactgga
      781 tgatcggcac cggcctgtac cgcgacgaca tcgattcggc gacctcgcag ctcgatgccc
      841 aggcctcgtc caacatcggc gagacgctgc tgtggatcgc agccatcgcg ttcggcggcg
      901 tggcgctgat cagcttcagc ggcatctggc tgaacctgag cgagctgcgc gtcgccgacg
      961 ccaagctgca gacgctcgcc cgcacggtgg tgcgcttcca ggaggacgag cgcgcccacc
     1021 tggcgcgcga gctgcatgac ggcgcgacgc agacgatggt gtcggccaag ctgctggtcg
     1081 aatcggccat cgaccagctg gaacgcgagg cgcgcgccgc cccgcgcgtg ctggagacgg
     1141 cgctcgccgg cctgaagcag tcgctcgacg aggtgcgccg catctcgcac cggctgcgtc
     1201 ccgcgatgct cgacgagctg ggcctgccgg ccgcgctgga gctgctcacg cgcgaggccg
     1261 aggacgccgg cgcgacgccc gtgcacctga gcgtgtccgg cgacgcgacg ccgctgcccg
     1321 aggaggtcaa gacggcgctg ttccgcgtca gccaggaggc gctggccaac gtcgccaagc
     1381 atgcgcacgc gcatcgcgtg gagatggcgc tggacttcgg cctggcgggc gtcgcgctgc
     1441 ggatcgacga cgacggccgc ggcttcgagg tcgaccgcat ccagctcgac cccgaccgcg
     1501 gcatcggcct gcgcaacatg cgcgaacgcc tggcttcgat cggcgggacg ttcgaggtgc
     1561 atgcctcgcc cggtgcgggc acgctgttgg tggccaaggt gccggcggac gcgatccggc
     1621 ggctggcggt tgcgggcaat gccgcgtaga ggcattggct cgcgaggcgc gacgcgtcgc
     1681 ctccggggtg gatcctgcga ctccgcttcg ctgcgcgcag gatgacggcc tggtgggggg
     1741 gcacggcgcg ccacaccaac gcttggcgag tccgctagaa caagctcgcc tgcgcctgcg
     1801 gctccgcccc gcgcggcctg aacagcgacg tgttcaacgt ccgccccgga aacgtcacgc
     1861 ccaaccgcgc ggccgcctcg tcataacgac gcgccagcgc ctcggccacg ctgcccgttc
     1921 ccaccatgcg gttgccgaag cgcgagtcgt agtccttgcc ctcgtgcatc cggtggatca
     1981 gccgccagac ctgcgcggcg cgctccgggt accagcgccg cagccactcg gcgaacaggt
     2041 cgcgcacctc gcggggcaga cgcagcagcg tgtacaggcc catcgtggcg ccgcgcgcga
     2101 cgcaggcctc taggatggcc tcgatctcgt gctcgttcag gctcgggatg accggcgcga
     2161 tgtagaccgc gaccgggatg cccgcgctcg tgagatcctc gatcgcctcg agccggcgcg
     2221 tgggcgagtt ggcgcgcggg tcgagccggc gcgagagctc gtggtccagc gtcgggatcg
     2281 acatgtgcac gcgcacgagt cccttgcggg ccatgggcgc gaggatgtcg atgtcgcgcg
     2341 tcaccagcgc cgacttggtc gtgatgccga cggcgacgtc gaactccgcg agcacctcca
     2401 gcacctggcg cgcgagcgcg tacttgcggt cgatcggctg gtaggcgtcc gtggcggcgc
     2461 ccagcgcgac gatgcccggg ttgaagcccc tgcggctcaa ttccttgcgc agcacctgcg
     2521 ccgcgttcat cttggcgaac agccgcgtct cgaagtccag gcccggcgac aggttcaggt
     2581 acgcatgcgt cggccgcgcg aagcagtaga cgcagccatg ctcgcagccc tggtaggggt
     2641 tgagcgtgag atcgaagtcg aggtcgggcg actggttgcg gttcaggatg gacttggcct
     2701 cgcgctcggt gacctcggtg cggcgcgcgg ccggatcgac gtcggcggcc gcgtcccact
     2761 cgtccttgac gtagcgcgac ggccggttcg agcccgcgcc gcgccccttg acgaagacga
     2821 cggg
//
LOCUS       DATCRO010000744         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_886054, whole genome shotgun sequence.
ACCESSION   DATCRO010000744 DATCRO010000000
VERSION     DATCRO010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886054"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..911
                     /locus_tag="VIN75_20175"
     CDS             453..911
                     /locus_tag="VIN75_20175"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HEY8976542.1"
                     /translation="MTPFHAPRLAALLVCASSLAQAAAGVPPSSAGTELHCPATLAQA
                     PVAEGLPNGWVLHGAPAELHVQRATFYDGDPVGLGTLAPDTTRRSGLTETSTWSFATS
                     DSARVWLGCLYGGATAIVARPLPAGLHQCTTVLRLTPLGDPSEPLSVRCR"
     gene            complement(1020..1796)
                     /gene="otsB"
                     /locus_tag="VIN75_20180"
     CDS             complement(1020..1796)
                     /gene="otsB"
                     /locus_tag="VIN75_20180"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8976543.1"
                     /translation="MSAGTQMLKPLLGPQGLAALAALVRAPALFAFDFDGTLAPIRPR
                     PDDVHVSATIALRLEKLARVRPVAIVTGRQIADVRERLGFTPSWIIGNHGAEDDGDPA
                     GAARARAALDALRERVRARADGLAGAGVLVEDKGQSLALHFRTAPDRAAASALIEEVM
                     AGFTSTLHVFGGKLVYNAVAADAPDKAVAVQRLIERAGAHAAFFAGDDVNDEPVFAAA
                     RPEWVTVRIGCERRESAARWCIDGPHEMASVLDRILAAIG"
     gene            complement(1796..4213)
                     /locus_tag="VIN75_20185"
     CDS             complement(1796..4213)
                     /locus_tag="VIN75_20185"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8976544.1"
                     /translation="MQTIRLQLRFLLPLIAVVIATAYLVLPLVDGVTLRWFSRDLDSR
                     GAVVANALSDSVVEAMRTNRMRQLEKLFDRAVQDERLFAIGLCSPDGRMLVRTPAFPA
                     DLNCFDALQISSKRDPRLVINGGAVHVAMHDVIDQPHPTPAAEAASEAVAAASAAVAD
                     GSASAAGDGSTVAAAVAASEATAQTPPVTVGRLVLLHDLSFIEKRSQDTRRYLVGLIA
                     VLGFVMALITMVVAQLSWRGWVNGVRALLRGEGIISPMGGGASSVASVELQPFAADLR
                     ERLRDLEDEYRRMQGPEADWSADRLHQLLMTQLRGDQVIVVSNREPYIHERRKDGSVV
                     VKRPASGLVTAVEPVMRACSGTWIAHGSGSADRDVVDSHDRVAVPPGHDDYQLRRLWL
                     TAEEEQGYYFGFANEGLWPLCHVAHVRPVFRESDWETYKAVNQRFADAVVAEARSEDP
                     VVLVQDYHFALLPAMVREKLPQATIITFWHIPWPNPESFGICPWRREILQGMLGSTIL
                     GFHTRFHCKNFIETVDRFLEARIEHEHSTISFREDETLVEAYPISIEWPDDTTIATWP
                     SVPEARRIVRERLNLHPDTTLAVGVDRFDYTKGILERLNAVERLLEKHPEWIERFVFV
                     QVAAPTRSSLEEYRSFQERVERLAQRINERFGRTGYQPVHLLAQHHDSHAVNELFRAA
                     DMCIVTSLHDGMNLVSKEFVAARDDEQGVLILSRFAGAAREMTEALIVNPYHVEETAD
                     ALHRAATMPAAEQRARMASLRATVREFNVFRWAGRMLTDAGRWRLKARVDARVARYRE
                     AAEKRGL"
     gene            4331..>4493
                     /locus_tag="VIN75_20190"
     CDS             4331..>4493
                     /locus_tag="VIN75_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY8976545.1"
                     /translation="MSNPPVVSNAHAASLDLAMIGNCAVSALVDARARVVWCCMPRFD
                     STPVFDALMQ"
BASE COUNT          629 a         1647 c         1564 g          653 t
ORIGIN      
        1 ggccgcgtgt cggctcgcgt ggaagccgcc gccgtggcct gacgcgcgcc gcgatcgcca
       61 ccgcgagcgc ggctcgtcgc accgccacca tcgccaccgc gcggttcacc gcgaccgtgt
      121 ccgcggccac ccgcgcaatc gtgtgatgcc cctcggctcg gcttcccctg cccgggcgta
      181 cggccgcatg cgagcgcctg caccgcgcgc gccgttcccg ccaacgctgc cgcgatgcgc
      241 ggcggtcgag tcggcgtgcg gcatgtcgag gtggcggggg aattcacgtc gagtccttcg
      301 ttcggggccc atgaatattg gcacggcagc ggcacgtccg ccccgcgccg aatcggcagt
      361 taacccagcg ttcggcgccg cggagtttca gccgatcggc cagagaatgg atcgatgacg
      421 cccgcattct tagaccgttc gccgttcctc gcatgacacc attccatgcc cctcgcctgg
      481 cggcgctgct ggtgtgcgcc agcagcctcg cgcaggccgc cgccggagtg ccgccctcgt
      541 ccgccggcac cgagctgcac tgccccgcca cgctcgcgca ggcgcccgtg gccgaaggcc
      601 tgcccaacgg ctgggtgctg catggcgcac cggccgaact gcacgtgcag cgcgccacgt
      661 tctacgacgg cgatccggtc ggcctgggca cgctcgcgcc cgacaccacg cgccgcagcg
      721 gcctcaccga aacctccacc tggtcgttcg ccaccagcga ctccgcgcgt gtctggctcg
      781 gttgcctgta cggcggcgcc accgccatcg tcgcgcggcc gctgcccgcc ggcctgcacc
      841 agtgcaccac ggtgctgcgc ctgacgcctc tgggcgaccc gtcggaaccc ctgtcggtgc
      901 gctgccgatg aagtgccagc cctcccttgt gcgacgtcgg ccgacgcggg cggccgtgcg
      961 caggtcgcga gcgccgcgca gcaggtgcag gtcgtcgccg tgacgccccg agcggccgct
     1021 cagccgatcg ccgcgaggat ccgatcgagc acgctcgcca tctcgtgcgg gccgtcgatg
     1081 caccagcgcg cggccgattc gcggcgctcg cagccgatgc gcaccgtcac ccattcgggc
     1141 cgtgcggcgg cgaacacggg ctcgtcgttg acgtcgtcgc ccgcgaagaa ggccgcgtgc
     1201 gctccggcgc gttcgatcag gcgctgcacg gccacggcct tgtcgggcgc gtcggcggcg
     1261 accgcgttgt agaccagctt gccgccgaac acgtgcaggg tcgacgtgaa gcccgccatc
     1321 acctcctcga tcagcgcgct ggccgcggcg cggtcgggcg cggtgcggaa gtgcagcgcc
     1381 agcgactggc ccttgtcctc gaccagcacg cccgcgccgg cgaggccgtc cgcgcgggcg
     1441 cgcacgcgtt cgcgcagcgc gtcgagcgcg gcgcgcgcgc gcgcggcgcc tgcgggatcg
     1501 ccatcgtcct ccgcgccgtg gttgccgatg atccacgacg gcgtgaagcc caggcgctcg
     1561 cgcacgtcgg cgatctgccg gccggtgacg atcgcgaccg gccgcacgcg cgccagcttc
     1621 tccagccgca gcgcgatggt ggccgacacg tgcacgtcgt cgggccgcgg ccggatcggc
     1681 gccagcgtgc cgtcgaagtc gaacgcgaac agcgccggcg cgcgcaccag cgccgccagc
     1741 gccgccaggc cctgcgggcc cagcaagggc ttgagcatct gcgttccggc cgacatcaca
     1801 gtcctcgctt ctcggccgcc tcgcgatagc gcgccacccg ggcgtcgacc cgcgccttca
     1861 ggcgccagcg gcccgcgtcg gtgagcatgc gcccggccca gcggaacacg ttgaactcgc
     1921 gcaccgtcgc ccgcaggctg gccatgcgcg cgcgctgctc cgcggccggc atcgtggcgg
     1981 cgcgatgcag cgcgtcggcc gtctcctcga cgtggtacgg attgacgatc agcgcctcgg
     2041 tcatctcgcg cgccgcgccg gcgaagcggc tgaggatcag cacgccctgt tcgtcgtcgc
     2101 gcgccgcgac gaactccttg ctgaccaggt tcatgccgtc gtgcaggctg gtgacgatgc
     2161 acatgtcggc cgcgcggaac agctcgttga cggcgtggct gtcgtggtgc tgcgccagca
     2221 ggtgcacggg ctggtagccg gtgcgcccga agcgctcgtt gatgcgctgc gcgagccgct
     2281 cgacgcgctc ctggaacgag cggtattcct ccagcgagct gcgcgtgggc gcggccacct
     2341 ggacgaacac gaagcgctcg atccactcgg gatgcttctc gagcaagcgc tccaccgcgt
     2401 tcaggcgctc caggatgccc ttggtgtagt cgaagcggtc cacgccgacg gccagcgtcg
     2461 tgtccgggtg caggttcagg cgttcgcgca cgatgcggcg cgcctcgggc accgaaggcc
     2521 aggtggcgat ggtcgtgtcg tcgggccact cgatcgagat cgggtacgcc tcgaccagcg
     2581 tctcgtcctc gcggaacgag atcgtcgagt gctcgtgctc gatgcgcgcc tcgaggaagc
     2641 gatccaccgt ctcgatgaag ttcttgcagt ggaagcgcgt gtggaagccc aggatggtcg
     2701 agcccagcat gccctgcagg atctcgcggc gccacgggca gatgccgaac gattcggggt
     2761 tgggccaggg gatgtgccag aacgtgatga tggtggcctg cggcagcttc tcgcgcacca
     2821 tcgcgggcag cagcgcgaag tggtagtcct gcaccagcac gaccggatcc tcgctgcgcg
     2881 cctcggccac caccgcatcg gcgaagcgct ggttgacggc cttgtaggtc tcccagtcgg
     2941 actcgcggaa caccggacgc acgtgcgcga cgtggcacag cggccacagg ccctcgttgg
     3001 cgaagccgaa gtagtagccc tgctcctcct cggccgtcag ccacaggcga cgcagctggt
     3061 aatcgtcgtg cccgggcggg acggcgacgc ggtcatgcga gtcgaccacg tcgcggtcgg
     3121 cgctgccgct gccgtgcgcg atccaggtgc ccgagcaggc gcgcatcacc ggctcgacgg
     3181 ccgtgaccag gccgctggcc ggccgcttga cgacgacgct gccgtccttg cggcgctcgt
     3241 ggatgtaggg ctcgcggttg gacaccacga tcacctggtc gccgcgcagc tgcgtcatca
     3301 gcagctggtg cagccggtcc gcgctccagt cggcctcggg gccctgcatg cggcggtact
     3361 cgtcctcgag gtcgcgcagg cgctcgcgca ggtcggcggc gaagggctgg agctccaccg
     3421 aggccaccga cgacgcgccg ccgcccatcg ggctgatgat gccctcgccg cgcagcagcg
     3481 cgcgcactcc gttgacccat ccgcgccagc tcagctgcgc caccaccatc gtgatcagcg
     3541 ccatcacgaa gcccagcacc gcgatcaggc cgacgaggta gcgccgcgtg tcctggctgc
     3601 gcttctcgat gaagctcagg tcgtgcagca gcacgaggcg tccgacggtg accggcggcg
     3661 tctgggccgt cgcctcggag gcggcaaccg cggccgcgac ggtcgaaccg tcgcctgcgg
     3721 cggacgcgct gccgtcggcc acggccgcac tggccgcggc gaccgcctcg ctcgccgcct
     3781 cggcggcagg cgtcggatgc ggctggtcga tgacgtcgtg catcgccacg tgcaccgcgc
     3841 cgccgttgat caccaggcgc ggatcgcgct tggagctgat ctgcagcgcg tcgaagcagt
     3901 tcaggtcggc cgggaacgcg ggcgtgcgca ccagcatgcg gccatcgggg ctgcacaggc
     3961 cgatggcgaa caggcgctcg tcctgcaccg cgcggtcgaa cagcttctcg agctggcgca
     4021 tccggttcgt gcgcatcgcc tccaccacgg agtccgacag cgcattggcc acgacggcac
     4081 cacggctatc gaggtcgcgc gagaaccagc gcaatgtgac accatcgacc agcggaagca
     4141 ccaggtacgc ggtggcgatc accacggcga tcagcggcag caggaaacga agttgcagcc
     4201 ggatcgtctg cacgatcggc caatacagga aggcgggccc ggcgcttgcc ggcggaacca
     4261 acgtcccgga ggttctgtct ttgttcaacg gaagatccat cttcttgaaa ccgaaggcgc
     4321 ccaaggaaac atgagcaatc ctcccgtcgt ttcgaacgcg cacgccgcat cgctggatct
     4381 ggcgatgatc ggcaactgcg ccgtcagtgc cctggtcgac gcccgggccc gcgtggtctg
     4441 gtgctgcatg ccgaggttcg acagtacacc ggtcttcgac gcgttgatgc agt
//
LOCUS       DATCRO010000745        23486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_221967, whole genome shotgun sequence.
ACCESSION   DATCRO010000745 DATCRO010000000
VERSION     DATCRO010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221967"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="VIN75_20195"
     CDS             <1..1884
                     /locus_tag="VIN75_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEY8976546.1"
                     /translation="TTHPPLLRIRRTPARPLAAALVALAIAGCATAPPPVAAPVDVKL
                     IAFNDFHGNLLSPGRLGEDARSPKRPVGGADALAGWIAHLEAGHPNHVVVGGGDFVGA
                     SPLVSGLFFDEPAVEVLGRAGVDFTSVGNHEFDKGATELLRLQHGGCRPEPGSCKGLG
                     STAPGHFDGARFQWLAANVVDTASGRTLLPPYGIKTFDGIPVAFIGMTLKGTPGIVTP
                     GGVAGLAFRDEADTVNALVPQLRAQGVEAIVVLVHQGGVQPGGSGDINGCDAALRNAD
                     GSESDIARIVRRLDDAVDLVVSAHTHAAYNCSANAVGGGATGLPNAAGRLVPVTSAAS
                     YGRVLTDVDLQLDRRTRDVVRVSPVNRLVVRDDPAVPVDAAVRDIVQGYVTLAAPVTD
                     RVLGELAQPLPNDPGDGGQAPAGSLVADGELHATQPAAAGAAQMAFVNPGGVRGAGFL
                     PPAGASAPFALTYGAAFTVQPFRNGLVTLTLTSAQLKALLEQQFDHCTDPLPQCALQL
                     SAGLRIKWSAGAPAGAKIIDVDWTPVDLGVVPPAVTGPTQAIVNAGVVLEPARRWRVT
                     VNGFLAGGGDGYSTLLAGTDRLQGGQELDALSAYLAPYRAPHAAYDAREPVLAAPRQV
                     RLP"
     gene            complement(1898..2512)
                     /locus_tag="VIN75_20200"
     CDS             complement(1898..2512)
                     /locus_tag="VIN75_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8976547.1"
                     /translation="MSAAPAPRLTDRKRDAIVQAAIVEFRDNGFEATSVDKVAARAEV
                     SKRTLYNHFASKDELFAAILHILWENSASDLQRAYEPGRPVREQLLELVHAKLVMLAD
                     ESFLALARVAIAAGLHSPERAREMVERIGEKDGAMLEWIRAAQKAGALRPADAAQAYK
                     QLECLVKGVAFWPQVAMGQPRLASAAQKKLATATVDLFLSHYGM"
     gene            2629..3699
                     /locus_tag="VIN75_20205"
     CDS             2629..3699
                     /locus_tag="VIN75_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8976548.1"
                     /translation="MTCLASRARRAPSPDAIDSPQHRDGKFRNVEPRRSPGFRKTLGI
                     AWRVLTQKPDTTVPRAPIPVRPLDAATLAAAPDASLFRLGHSTLLLKLAGEFWLTDPV
                     FSERASPVQWAGPKRFHAPPISIAELPPIKGVILSHDHYDHLDHAAVLELAPKVEMFI
                     TPLGVGDRLIGWGIPPEKVRQLDWWQQTSLAGVRLVATPAQHFSGRGLGDGDSTLWAS
                     WVILAGDLRVFFSGDTGYHAGFKAIGERFGPFDVTFMETGAYDAQWPEVHMQPEESLQ
                     AHLDVRGRWMMPLHNGTFDLAMHAWHEPFDRILALAQARGVPLATPAMGERLSLAQPH
                     AGERWWQSVETSLQAAAAAQAA"
     gene            3713..4381
                     /locus_tag="VIN75_20210"
     CDS             3713..4381
                     /locus_tag="VIN75_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8976549.1"
                     /translation="MTISVLITGATGMVGEGVLLECLADPRVARVLVVNRKPLGRTHP
                     KLTEIIHGDFHDLSAIEGRLAGIDACFFCLGVSSIGMDEHDYRHVTYDLTVGFARALA
                     RLNPDMAFCYVTGAGTDSTEHGQVMWARVKGATENELLRLFRRAVMFRPAMMRATAGQ
                     KNLKGWYKALAWIYPIGRALAPGKFCTLQEVGRAMINAASAGAPRHVLEVRDIVDLAG
                     MRAS"
     gene            4448..4981
                     /locus_tag="VIN75_20215"
     CDS             4448..4981
                     /locus_tag="VIN75_20215"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY8976550.1"
                     /translation="MPVASQRIALPVVLVLMGVSGSGKTTVAKLIADRLGWAFEEGDA
                     LHPAANVAKMAAGHPLDDDDRAPWLAKVADWVDARLDAGHSGVITCSALKRKYRALID
                     RRGEGVEFVFLHGSRELVASRLSTRQGHFMPSSLLASQFETLEEPGDGEPVIRVEIDG
                     TPEAIASAVLDALGLAA"
     gene            5004..6392
                     /locus_tag="VIN75_20220"
     CDS             5004..6392
                     /locus_tag="VIN75_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEY8976551.1"
                     /translation="MAADLAHPVLNPHDTQVLVVAAIGIALIVAMIVWLKLHAFIALT
                     VGALFVGAGSGIPLAKVTASYETGAGGVLGYVGVLIALGAMVGKLLADSGGADKVVET
                     LLRGRPATLPWKMALIASIIGIPMFFEIGLVLLIPVVMLAVHRSQGLAMRLGIPALAG
                     LSVLHGFIPPHPGPLAAIAILHANVGVTLALGLLIAVPAVAIAGPVYGAIAARLVPVG
                     AAGAGLAVTQGGKSDDADARASRSPSFRVIMATLLFPLALMLLKAGADIFLDKDSPAR
                     PLLDFIGDPVFALLAAVLLAMVTFGTAVGFSPAVLGKKIGASLAPVVGVMLIVGAGGG
                     FKQSLVDGGTGAAIAKIALAAGLSTLLLGWIVAVLIRLATGSATVATVTAAGIVAPLA
                     AGLPPTQLSLVVLAVGAGSLFFSHVNDAGFWLVKEYFGLTVGQTIKTWSIMETVISVT
                     GLALAGGLSLVL"
     gene            6577..8460
                     /locus_tag="VIN75_20225"
     CDS             6577..8460
                     /locus_tag="VIN75_20225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8976552.1"
                     /translation="MNVPHEQPIPPGQSASRLLRDRRLVQATWAACLAIVASSIPDFF
                     QAQWRNLPPLFVGLAAMGLVLWLVRNHRRGAAVALLLVSLMGMVGLLMWQNGGLRDTS
                     LLAFPCLLVLAAMMGSGRLYLALFVTMIALVGVVLLANVNGWHVNYVPPLSANTFVDI
                     AAVLSVTCVIAWLMAGDMRSALDAVNAQNAQMAATQARMALMATHDALTALPNRSLVR
                     DRFEQAAAIARRAGHGVAMLYLDLDNFKNVNDTLGHASGDTLLRQVGDRLSALLRTAD
                     TVARLGGDEFLLLVPEVTDADSVGDIASKIVTGLMAPFDVIGMEIFTGCSLGVTMFPA
                     DGDDFDSLLKKADIAMYRAKESGRNAYRFWDGEMNASVVEHVELLSALRTALAHREFT
                     LSYQPQFDLRTGRLIGAEALIRWRHPDKGPISPARFIPLAERSGLIVPIGDWVLQEAC
                     AQAQRWRAAGWPDLTISVNVSPVQFRRGSVETSVAQALADSGLPSANLELELTESLLI
                     QDSLQLAESLASLRRMGVRFAIDDFGTGYSNLAYLKRFEVERLKIDQTFVRRLTDDVQ
                     DQAIVRAIVQMASSLNLYTVAEGVEDGATLALLGDLGCQQGQGFHWSPAVPAADFEAF
                     LRS"
     gene            complement(8588..10273)
                     /locus_tag="VIN75_20230"
     CDS             complement(8588..10273)
                     /locus_tag="VIN75_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976553.1"
                     /translation="MRAITSWIALGAATTLLAGCGGGYDDGYDGGDGGGELISYVGTT
                     GVFAAWADSATGEYAAADMGTYAGKRQSLHGTIDFLTGQDLMQPAGVEIWKTSDGHIR
                     AIDLASFFEPHSQQISNETAATVDATCSLTGTQVAGASVDYAGVYFASDLQDPTNSAY
                     FYRLPGLDGNCNTGDDVIHMVRTGMSSTDAPITVSAMPVATVRSATGSITGFVVKQGA
                     QLVRVDNNFANPVVLGTFASAIGVALALPVGTTQGYPTGQLFIVDGNIVRVDYAAATT
                     SPPLFTIPNWTPTNNGALFAASPTTLYVGVNTPAAGSTPASASIVAIPADGSAAGTVV
                     ATEAAHLDSLAFPVGGTNLVWSETTPLYTVRALAQSGGSPFTVVASSVNGGSFTATAT
                     TVYYTTWSSVTNASARTVVRSQTASGIVGLDGSVIQAPMANSSFAGGGEQQPWPDDTV
                     TTATPLETVLQVQNLTPVTVTDPANGYAYTVDGVSGGLVVAIDTATNRAGATIGRLPA
                     SNAAWLTGTFRDSGHTGFVEATNAASTADPATRDLYLLNTQQDDTLIRVTGNL"
     gene            complement(10678..11571)
                     /locus_tag="VIN75_20235"
     CDS             complement(10678..11571)
                     /locus_tag="VIN75_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976554.1"
                     /translation="MIRTRIAFAGLAVAAAGLALAGCAALTRLGGRPIGPCGFDVETL
                     QFAGDALAQARCLLRPVAQGGVVSTSPAVLPDPLASLVGQPVGDLGPRLQRYLAAHAI
                     PDAAIGGPLAAPLSRARDGDAAAPAARYFVLHDTSTPWLGDAAAFPPDDDPSLNALAR
                     FAGPEAVAHVFVNRKGETLTGHGFDVPWRATQLEMRRIGPRAKGLFLHVELAQPRRRD
                     PAGAPGNDAIAPTPGFTDAQYAQAALLYAAASARRGDWLIPATHAALDQGIAGAHDDP
                     QHFELARLALALDRLRDALGR"
     gene            complement(11595..12911)
                     /locus_tag="VIN75_20240"
     CDS             complement(11595..12911)
                     /locus_tag="VIN75_20240"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8976555.1"
                     /translation="MRTRPPRALGALAIAVVAVASLLPIARLAQACSGNAPEPSGTET
                     HFPLMYGDRPTVAQLSDLGRRIFMDPAYSASGKQSCASCHDPATAFGPPNDRAVQPGG
                     PHLDRTGFRNTPSLRYVHAPVAFTQHFLESQVTLGVDDEGPTGGRTWDGRVDTAHDQA
                     LMPLMDPNEMANADGAAVVKRLKTTSYADEFRQVLSAPGENVFDDPDSTLVWVTVALE
                     TYEQSAPEFHPFDSKFDAWLNDKATLSPAEARGMALFNDMKKGNCASCHPSTRKAAAN
                     RPPIFTDFGFVAIGVPRNRELPANRDPTFFDLGLCGPLRTDLAAHPEYCGMFRTPSLR
                     NVALRRRFFHNGAIRTLRDAVAFYATRDTDPARWYPGSHGQRGYDDLPRQYWGNVSHE
                     VPFEPLPGGRPRLDDREIDDIVAFLRTLTDGWKPPAKAPAKAVAAR"
     gene            complement(12916..13509)
                     /locus_tag="VIN75_20245"
     CDS             complement(12916..13509)
                     /locus_tag="VIN75_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976556.1"
                     /translation="MDKTLITAALLALAPLAQAATVVDTGAPSGSPVGSLVFDGSDWA
                     AAQVSFANASTIAAIAGHILGGTSGETFDISLYDGGARPGAQLFTTTATYGADGWNGA
                     SGLAWTVAPGSYWIEFEVNGNDTLGTTPTATGALLDTGVARPVSTATTSDWGFTYDAS
                     AASIGLQVSVVPEPAGALQVLAGMLLLTSLGLARRRR"
     gene            complement(13518..14744)
                     /locus_tag="VIN75_20250"
     CDS             complement(13518..14744)
                     /locus_tag="VIN75_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976557.1"
                     /translation="MTHHTARNAALLAIAAAVAAPAAHAQDTTPVVLTFSTVGDSRQD
                     PVKYDQASVGPTLSGQDATWLQNTKAFTRILRTIQSQKSTMLFFNGDMIHGYGWANYG
                     YTSNATQSAIAGPVAPATTSDIVGSDLMKEYRQYAFWRGLVAPLPETGTYVFPVPGNH
                     ETECKACGKNSDGQTQAKVENEQAYAANMGDLILDTARFTSVLGAAPANVNYGPAAGV
                     GPDALPTDQSKLSYSFDYQGLHFAVINTDAVGAETSAPTAWLAADLAAAQGRGVKRFF
                     VFGHKPAFTYAYAPGVSGSGLDATATTTARRDAFWNVIEQYKATYFTGHMHTYNISQP
                     KGGAYQVIVGAGGSPFDPSAAQATAGVLAHPATDRDYAWATVKVHADGGVDILGYGFS
                     STFGPTQLLGQYFIAP"
     gene            complement(14910..15245)
                     /locus_tag="VIN75_20255"
     CDS             complement(14910..15245)
                     /locus_tag="VIN75_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976558.1"
                     /translation="MRRRSAAALVAGLAGAGCVIVPVQMEDYDPGCRLVTHHIELQSV
                     VLGQLNSCSGQGCEAVVLASLGVTAVSAIVSGSIAIIGNVAYWAEHRAGCAVPPPAPV
                     AAPGAPPID"
     gene            complement(15256..15585)
                     /locus_tag="VIN75_20260"
     CDS             complement(15256..15585)
                     /locus_tag="VIN75_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976559.1"
                     /translation="MKLTRIVPAVAAGLLASGCVVVPMTRDSYDDDCKVATHHMELTS
                     VQLKEVVGCSGQTQFFQPFCVLGAVGLATVSTVVSGSVVVVGNVVYWSERKAACVAPS
                     LGIRPDA"
     gene            complement(15605..16480)
                     /locus_tag="VIN75_20265"
     CDS             complement(15605..16480)
                     /locus_tag="VIN75_20265"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8976560.1"
                     /translation="MPLHLPTYLLYGEDAGLPPEDVMHSESIAERSALHSWEIKPHRH
                     EALLQVFWFEKGQVEMLIDGHAHSLRGPGVILVMPLAVHGFRWTSDVKGIVFTMRESH
                     VRTLLARDESLRERVLVSRCVQFAPAHRKAVKTAVEALHEEQTQHAPWRASAIDAAMT
                     ALFVALGRSLPAPAAAAGVGDRSVVHVQRFRALVDERFREQPTLGEFAAEIGVTTTQL
                     NRVCRSVLGHPALAVLHARLCLEAQRELAYTTLSVKHIAYKLGFSDAGYFTRFFERET
                     GHSPSTWRKEQLARQ"
     gene            16674..17561
                     /locus_tag="VIN75_20270"
     CDS             16674..17561
                     /locus_tag="VIN75_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY8976561.1"
                     /translation="MPASTPCIISVAITGSLPRKKDNPAVPVTVSEQVESTHAAYEAG
                     ATLVHLHVRNDDETPTSAPERFAQVLDGIRKHCPLIITQVSTGGRSGAGRERGGMLHL
                     RPDMASLAPGSVNFPTRVYDNAPDLVDWLASQMRAYGVKPEIEAFDLSMIFQAAAMAR
                     DGRIEGPLHVQFVMGVKNAMPVDREVLEFYVKTLHRLAPDATWTGAGIGRDQLTLAKW
                     SLELGGHCRTGLEDNVRLDRETLAPSNAALVRQLAGLCEQAGRRVATPAEARALLRLA
                     PQPGARTAAQLHAEHVAHA"
     gene            17584..18360
                     /gene="pcaH"
                     /locus_tag="VIN75_20275"
     CDS             17584..18360
                     /gene="pcaH"
                     /locus_tag="VIN75_20275"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEY8976562.1"
                     /translation="MHATAPLARPDDEYLMRDPASHPPAYAPGYKTSALRSPRNALIS
                     IGQTLSEVTAPVFRAEELGPLDGDLIRNYAKTGLPIGERAIVHGYVRDQLGRPVRNAL
                     VEVWQANASGRYRHKKDQYLGALDPNFGGCGRVLTGDDGHYVFRTIKPGPYPWRNRIN
                     DWRPAHIHFAISGSGWAQRLVTQMYFEGDPLISTCPIIRTIADEEQIRGLIAQLDLAA
                     TVPLDSRAFRFDIVLRGQRATWFENVLQSDGQASHEGGVR"
     gene            18357..18977
                     /gene="pcaG"
                     /locus_tag="VIN75_20280"
     CDS             18357..18977
                     /gene="pcaG"
                     /locus_tag="VIN75_20280"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY8976563.1"
                     /translation="MTPLLRETASQTAGPYVHIGLAPRAAGFDIFENDFGPVLTTPGT
                     RGERITIEGRVFDGSGTPLRDVLLELWQANAQGRYAHAADRQPGKALDDAFRGWGRAC
                     SDFETGVWSFETIKPGAVAGRHGVAMAPHVSLWIVARGINIGLHTRMYFDDEAQANAA
                     DPVLNLVEWEVRRKTLVGRREQRDGRTIYRFDIHLQGDDETVFFDV"
     gene            18985..20394
                     /gene="pcaB"
                     /locus_tag="VIN75_20285"
     CDS             18985..20394
                     /gene="pcaB"
                     /locus_tag="VIN75_20285"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEY8976564.1"
                     /translation="MIFDRFLASPEVMQVLDERAFVQAMLDFEAALARAQAAAGAIPP
                     GAGEAIAAACDARGFDVDAIVAEGARVGTIAIPLVKALTAAVAKADADAALVVHWGGT
                     TQDVLDTAQVLVTRRALALIDRDLSTLVDALLRLAKTHGDAPLLGRTLMQPAQVISFG
                     FKLAAWVAPLVRAQERLRLAGDSALQLQLGGAVGTRAALGEHGDAVAAGMARLLDLRL
                     PPAAWHTQRDELVALGCELGVLAGSLGKIGKDIALMAQGEVGEIAEPSGGGRGGSSAM
                     PHKRNPVAAMNALAGAVRAPQRVAALLAAMPQEHERGLGNWQAEPAEFGALLAGVHGA
                     LASLAFAAGGLQVSAQRMRANIDALQGLVFAEALSMRVAREIGKSEAHHWVEGLAGDV
                     VAGGAQLHDVALAALARHARLRERISAGELAALFDPDAPARHASALAAPQLAALATHA
                     EELRRDPPWRDHLQGSHHA"
     gene            20387..21598
                     /locus_tag="VIN75_20290"
     CDS             20387..21598
                     /locus_tag="VIN75_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8976565.1"
                     /translation="MPDREDDFDRGLKNRRAVLGDAWVDKSLDGANAFNADFQSLITR
                     YAWNDIWGRPGLDHTTRRLLVLGMTMGLARWEEFELHAGAAIRAGVPLEKIQETLMQG
                     AIYCGVPAANTAFKIMIDQLREHGLLPGPDPLGAGTRVATHHTFSVPQLKVALQGRGA
                     PVVLSHALGLDMAMWDDLAARLAAGGLGAPREVLRFDHRGHGGSAAPAGPYAMDELVD
                     DAARLVREWGRGPVTWVGLSMGAMVGMGLAIRHPELVDRLVLANTTSRYPDAAQSAWS
                     QRIATVNASGMAAVAEMVVERYLHADFRAARPEATQAVRATILRNDPAAYAASCAAVA
                     GVAWQDRLATVRCPTLVISGARDAGAPPAMGQAIAERVPGARLEVIADASHLSVLETP
                     EAFEALLRPFL"
     gene            complement(21896..>23486)
                     /locus_tag="VIN75_20295"
     CDS             complement(21896..>23486)
                     /locus_tag="VIN75_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEY8976566.1"
                     /translation="VAATASTDCYNWGYDPFHFTAPEGSYATSVADGSTRVVEFRRMV
                     QSLNQAGLRVGMDVVFNHTTASGQNDHSVLDRVVPGYYHRLDANGNVLRDSCCDDTAT
                     ENMMMGRLMTDSVVTWARDYHVSSFRFDIMGLQPRDTMVALQAKVDAAVGHHVELIGE
                     GWNFGAVANGARFVQADMLDLNGTGIGTFNPAIRDAIRGGGCCDSGGALMSNQGYING
                     LWYDPNPQAGGHGAGDLMWLGDVIKASLAGSIRSYSLTTSWDAQLPLEQISFEGLPAG
                     YVTNPSEVVNYFENHDNLTIFDNNVYKLPTTTSREDRARVQMLGAAINAFSQGVAYFH
                     AGVDTLRSKSLDGNSYDSGDWFNRLDWSYQDDNFGVGLPPAGSNQSNWTMQQPLLANA
                     DIKPTPVEIAWARDEFRDLLKIRRSSTLLRMASADDIKARLKFFNTGSRQIPTLLVGD
                     LDGRGYAGANFQELTYFVNVDKAAHAITIPALAGKAFQLHPVHAGFAAADKRAAQATV
                     DTASGTFTIPARTAVVFVVKQ"
BASE COUNT         3213 a         8349 c         8447 g         3477 t
ORIGIN      
        1 accacgcacc cgccgctgtt gcgcatccgc aggacgcccg ctcgcccgct ggccgccgcc
       61 ctcgtcgccc tggcgatcgc cggctgcgcc accgccccgc ccccggtcgc cgcgccggtc
      121 gacgtcaagc tgatcgcctt caacgacttc cacggcaacc tgctgtcgcc cggccgcctt
      181 ggcgaggacg cgcgatcgcc caagcggccc gtgggcggcg ccgacgcgct cgcgggctgg
      241 atcgcgcacc tggaggcggg ccatccgaac cacgtggtcg tgggcggcgg cgacttcgtc
      301 ggggcgtcgc cgctcgtctc ggggctgttc ttcgacgagc cggccgtcga ggtgctcggc
      361 cgcgccggcg tcgacttcac gtccgtcggc aaccacgagt tcgacaaggg cgcgacggag
      421 ctgctgcggc tgcagcacgg cggctgccgg cccgagcccg gcagttgcaa gggcctgggc
      481 tcgaccgcgc ccggccactt cgacggcgcg cgcttccagt ggctggcggc caacgtcgtc
      541 gacaccgcca gcggccgcac gctgctgccg ccgtacggca tcaagacctt cgacggcatt
      601 cccgtggcct tcatcgggat gacgctcaag ggcacgcccg gcatcgtcac ccccggcggc
      661 gtggcgggcc tggcgttccg cgacgaggcc gacaccgtca acgcgctggt gccgcagctg
      721 cgcgcccagg gcgtcgaggc catcgtcgtc ctcgtgcacc agggcggcgt gcagcccggc
      781 ggcagcggcg acatcaacgg ctgcgacgcc gccctgcgca acgccgacgg cagcgaatcg
      841 gacatcgcca ggatcgtgcg ccgcctcgac gacgcggtcg acctcgtcgt cagcgcgcac
      901 acgcacgcgg cctacaactg ctcggccaac gcggtgggcg gcggcgcgac gggcctgccc
      961 aacgccgcgg ggcgcctggt gcccgtcacc agcgccgcgt cgtacggccg cgtgctgacc
     1021 gacgtcgacc tgcaactgga ccggcgcacg cgcgacgtgg tgagggtctc gcccgtcaac
     1081 cggctggtcg tgcgcgacga tccggcggtg ccggtcgacg ccgcggtgcg cgacatcgtg
     1141 cagggctacg tgacgctcgc cgcgccggtc accgatcgcg tgctcggcga gctggcgcag
     1201 ccgctgccca acgatcccgg cgacggcggc caggcgcccg cgggcagcct cgtggccgac
     1261 ggcgagctgc acgcgacgca gcccgcggcg gccggcgcgg cgcagatggc cttcgtgaac
     1321 ccgggcggcg tgcgtggcgc cggcttcctg ccgccggccg gcgcgagcgc gcccttcgcg
     1381 ctgacctacg gcgccgcctt cacggtgcag ccgttccgca acgggctggt gacgctgacg
     1441 ctgacgtcgg cgcagctcaa ggccctgctc gaacagcagt tcgaccactg cacggacccg
     1501 ctgccccagt gcgcgctgca gctgtcggcg ggcctgcgca tcaagtggag cgccggggcg
     1561 ccggcgggcg cgaagatcat cgacgtcgac tggacgccgg tcgacctggg cgtggtgcca
     1621 ccggcggtca ccgggccgac gcaggcgatc gtcaacgcgg gcgtggtgct ggagccggca
     1681 cgccggtggc gcgtcacggt caacggcttc ctggccggcg gcggcgacgg ctattccacg
     1741 ctgctggcgg gcaccgaccg gctgcagggc gggcaggaac tggacgcgct gtcggcctac
     1801 ctggcgccat atcgtgcgcc gcatgcggcc tacgacgcgc gcgagccggt gctggccgcg
     1861 ccgcgccagg tgcgccttcc gtgacgccgg tgggccgcta catcccgtag tggctcagga
     1921 acaggtcaac cgtcgcggtc gccagcttct tctgcgcggc cgaagccagc cgcggctggc
     1981 ccatcgccac ctgcggccag aaggcgacgc ccttgaccag gcattcgagc tgcttgtacg
     2041 cctgcgcggc atcggccggc ctgagcgcgc cggccttctg cgccgcacgg atccactcca
     2101 gcatcgcgcc gtccttctcg ccgatgcgct cgaccatctc gcgcgcccgc tccggcgaat
     2161 gcagccccgc ggcgatggcc acgcgtgcca gcgccagaaa gctctcgtcg gccagcatca
     2221 cgagcttggc gtgcaccagc tccagcagct gctcgcgcac cggccggccc ggctcgtacg
     2281 cgcgctgcag gtcgctggcg ctgttctccc acaggatgtg caggatcgcg gcgaacagct
     2341 cgtccttgct cgcgaaatgg ttgtagagcg tgcgcttgga cacctccgcg cgcgccgcca
     2401 ccttgtcgac gctggtcgcc tcgaagccgt tgtcgcggaa ctcgacgatg gccgcctgca
     2461 cgatggcgtc gcgcttgcgg tcggtgaggc ggggagcagg agcggcggac atgacagaac
     2521 tttacaccag gcagtttact ttttggcgga tcggccgtac actgaaaaac tacacggtgc
     2581 agtttactta ttgatccgaa ccgcgccggc cttccgaagg aaagcgccat gacctgcctc
     2641 gcctcgcgcg cccgtcgcgc accctcgccc gatgccatcg actcgccgca gcaccgcgac
     2701 ggcaagttcc gcaacgtgga gccgcgccgc tcgcccggct ttcgcaagac gctgggcatc
     2761 gcctggcgtg tgctgaccca gaagcccgac accaccgtgc cgcgcgcccc gatcccggtg
     2821 cggccgctgg acgccgcgac gctggccgcc gcgcccgacg ccagcctgtt ccgcctgggc
     2881 cactccacgc tgctgctgaa gctggccggc gagttctggc tcaccgaccc ggtgttctcg
     2941 gagcgcgcct cgccggtgca atgggccggc ccgaagcgct tccatgcgcc gccgatctcg
     3001 atcgccgagc tgccccccat caagggcgtc atcctctcgc acgaccacta cgaccacctc
     3061 gaccacgccg cggtgctcga gctcgcgccc aaggtggaga tgttcatcac gccgctgggc
     3121 gtgggcgacc gcctgatcgg ctggggcatc ccgccggaga aggtgcgcca gctcgactgg
     3181 tggcagcaga cctcgctggc cggcgtgcgc ctcgtcgcca cgcccgcgca gcacttctcg
     3241 ggccgcggcc tgggcgacgg cgactcgacg ctgtgggcgt cgtgggtgat cctggccggc
     3301 gacctgcgcg tgttcttcag cggcgacacg ggctaccacg cgggcttcaa ggcgatcggc
     3361 gagcgcttcg gcccgttcga cgtgacgttc atggagaccg gcgcctatga tgcgcaatgg
     3421 cccgaggtgc acatgcaacc cgaggaatcg ctgcaggcgc acctggacgt gcgcggccgc
     3481 tggatgatgc cgctgcacaa cggcaccttc gacctcgcga tgcatgcgtg gcacgagccg
     3541 ttcgaccgca tcctcgcgct ggcgcaggcg cgcggggtgc cgctggccac gcccgcgatg
     3601 ggcgagcgcc tgtcgctcgc gcagccgcac gcgggcgagc gctggtggca gtcggtcgag
     3661 acgtccctgc aagccgcggc cgctgcccag gccgcctgag gaaggcgctt ccatgacgat
     3721 ctcggtcctc atcaccggcg ccaccggcat ggtcggcgaa ggcgtgctgc tcgaatgcct
     3781 ggccgacccg cgcgtcgcac gcgtgctggt cgtcaaccgc aagccgctgg ggcgcacgca
     3841 tccgaagctc accgagatca tccacggcga cttccacgac ctgtcggcga tcgagggccg
     3901 gctcgccgga atcgacgcct gcttcttctg cctgggcgtg tcgtcgatcg gcatggacga
     3961 gcacgactac cggcacgtca cctacgatct caccgtcggc ttcgcccgcg cgctcgcgcg
     4021 cctgaacccg gacatggcgt tctgctacgt caccggcgca ggcaccgatt ccaccgagca
     4081 cggccaggtg atgtgggcac gcgtcaaggg cgccaccgag aacgagctgc tgcgactgtt
     4141 ccgccgcgcg gtgatgttcc ggccggcaat gatgcgcgcc acggcgggcc agaagaacct
     4201 gaagggctgg tacaaggcgc tcgcctggat ctacccgatc ggccgcgcgc tcgcgccggg
     4261 caagttctgc acgttgcagg aggtgggccg cgcgatgatc aacgcggcct cggccggcgc
     4321 gccgcgccac gtgctcgagg tgcgcgacat cgtcgacctg gcgggcatgc gggcaagctg
     4381 acgcgcgcgg gcacggcgcg gcgtccggac gtatgctcgc gacgtccacc gcacccccgc
     4441 ccgccccatg cctgtcgcct cccagcgcat cgccctgccc gtcgtcctcg tcctgatggg
     4501 cgtgtccggc agcggcaaga ccaccgtcgc caagctcatc gccgaccgcc tgggctgggc
     4561 cttcgaggaa ggcgacgcgc tgcacccggc ggccaatgtc gccaagatgg cggccggcca
     4621 tccgctggac gacgacgacc gcgcgccctg gctcgccaag gtggccgact gggtcgacgc
     4681 gcggctggac gccggccact cgggcgtcat cacctgctcg gcgctcaagc gcaagtaccg
     4741 cgcgctgata gaccgccgcg gcgagggcgt ggagttcgtc ttcctgcacg gttcgcgcga
     4801 gctggtcgcg tcgcgcctgt ccacgcgcca gggccacttc atgccctcct cgctgctggc
     4861 cagccagttc gagacgctgg aggaacccgg ggacggcgaa cccgtgatcc gcgtcgagat
     4921 cgacggcacg cccgaggcca tcgccagcgc cgtcctcgat gcactcggcc tcgcggcctg
     4981 acccatcccg aaggagacgc cgcatggccg ccgacctcgc ccatcccgtg ctcaacccgc
     5041 acgacacgca ggtgctggtc gtcgcggcga tcggcatcgc gctgatcgtc gcgatgatcg
     5101 tctggctcaa gctgcacgca ttcatcgcgc tgacggtcgg cgcgctgttc gtcggcgccg
     5161 ggtcgggcat tccgctcgcc aaggtcaccg cctcgtacga gacgggcgcc ggcggcgtgc
     5221 tgggctacgt gggcgtgctg atcgcgctgg gcgcgatggt gggcaagctg ctggccgact
     5281 ccggcggcgc cgacaaggtc gtcgagacgc tgctgcgtgg acgtcccgcc acgctgccgt
     5341 ggaagatggc gctgatcgca tcgatcatcg gcatcccgat gttcttcgag atcggcctcg
     5401 tgctgctcat cccggtggtg atgctggcgg tgcaccgctc gcaagggctt gcgatgcggc
     5461 tgggcatccc ggcgctcgcg ggcctgtcgg tgctgcacgg cttcatcccg ccgcacccgg
     5521 gtccgctggc cgcgatcgcg atcctgcacg ccaacgtcgg cgtcacgctg gcgctgggcc
     5581 tgctgatcgc ggtgccggcg gtggcgatcg ccgggcccgt gtacggcgcg atcgccgcgc
     5641 ggctggtgcc ggtgggcgcc gccggcgcgg gcctggccgt gacgcagggc ggcaagtccg
     5701 acgacgccga cgcgcgcgcg tcgcgcagcc cgagctttcg ggtcatcatg gccacgctgc
     5761 tgttcccgct ggcgctgatg ctgctcaagg cgggcgccga catcttcctc gacaaggact
     5821 cgccggcccg cccgctgctc gacttcatcg gcgaccccgt cttcgcgctg ctggccgcgg
     5881 tgctgctggc gatggtgacc ttcggcaccg ccgtcggctt ctcgcccgcc gtgctgggca
     5941 agaagatcgg cgccagcctc gcgccggtgg tcggcgtgat gctgatcgtc ggcgcgggcg
     6001 gcggcttcaa gcagtcgctg gtcgacggtg gcaccggcgc ggcgatcgcc aagatcgcgc
     6061 tggccgccgg cctgtcgacg ctgctgctgg gatggatcgt ggccgtgctg atccgcctgg
     6121 ccacgggctc ggcgacggtc gccaccgtca ccgcagccgg catcgtcgcg ccgctggccg
     6181 ccggcctgcc gccgacgcag ttgtcgctcg tcgtgctcgc ggtgggcgcg ggctcgctgt
     6241 tcttctcgca cgtcaacgac gcgggcttct ggctggtcaa ggaatatttc gggctgacgg
     6301 tgggccagac gatcaagacg tggtcgatca tggagaccgt gatctccgtc accggattgg
     6361 cgctggcggg cggcctttcg ctggtcttgt gatgccaaca atgaccggat ttcgtgaaac
     6421 tcgataccta ccggggtaga cagccttgtc acgcgtgcaa cactccgctg gctgacggga
     6481 acctcgaccg gacggggact tcgcgcggca cgccgacgcc tggcgcttcg agcctgggca
     6541 agtgacagac gcgacgagtc caagaggtcc gaaccggtga acgtacccca cgagcagccg
     6601 atcccgccag gccagagcgc atcgcgcctg ctgcgcgacc gacgcctcgt gcaggccacc
     6661 tgggccgcct gcctcgcgat cgtcgcctcc tcgatccccg acttcttcca ggcgcagtgg
     6721 cgcaacctgc cgccgctgtt cgtgggcctg gccgccatgg gactcgtgct gtggctggtg
     6781 cgcaatcacc gccgcggcgc ggccgtcgcc ctgctgctgg tctcgctgat gggcatggtc
     6841 ggactgctga tgtggcagaa cggcggcctg cgcgacacgt ccctgctggc cttcccgtgc
     6901 ctgctggttc tcgccgcgat gatgggatcg ggccggctgt acctcgcgct gttcgtgacg
     6961 atgatcgcgc tggtgggcgt cgtcctgctt gccaacgtca atggctggca cgtgaactac
     7021 gtgccgccgc tgtcggccaa caccttcgtc gacatcgccg ccgtcctctc ggtcacctgc
     7081 gtgatcgcgt ggctgatggc cggcgacatg cgcagcgccc tcgacgccgt gaatgcccag
     7141 aacgcgcaga tggcggcgac gcaggcgcgc atggcgctga tggccacgca cgacgcgctc
     7201 accgccctgc ccaaccgcag cctggtgcgc gaccgcttcg aacaggccgc ggccatcgcg
     7261 cgccgcgccg ggcacggcgt ggcgatgctg tacctggacc tggacaactt caagaacgtc
     7321 aacgacaccc tgggccacgc ctccggcgac accctgctgc gccaggtggg cgaccgcctg
     7381 tccgccctgc tgcgcacggc cgacaccgtg gcgcgcctgg gcggcgacga gttcctgctg
     7441 ctggtgcccg aggtgaccga cgccgactcg gtaggcgaca tcgcgagcaa gatcgtcacc
     7501 ggcctgatgg cgccgttcga cgtgatcggc atggagatct tcaccggctg ctcgctgggc
     7561 gtgacgatgt tcccggccga cggcgacgac ttcgacagcc tgctcaagaa ggccgacatc
     7621 gcgatgtacc gcgccaagga gtcgggccgc aacgcctacc gtttctggga cggcgagatg
     7681 aacgccagcg tcgtcgagca cgtcgagctg ctgtcggcgc tgcgcacggc gctcgcgcac
     7741 cgcgagttca cgctgtccta ccagccgcag ttcgacctgc gcaccggccg cctgatcggc
     7801 gccgaggcgc tgatccgctg gcgccatccc gacaagggcc cgatctcgcc ggcgcgcttc
     7861 atcccgctgg ccgagcgctc gggcctgatc gtgccgatcg gcgactgggt gctgcaggag
     7921 gcctgcgcgc aggcgcagcg ctggcgcgcg gcggggtggc cggacctgac gatctcggtg
     7981 aacgtctcgc cggtgcagtt ccgccgcggc agcgtcgaga cctcggtggc gcaggcgctc
     8041 gccgactccg gcctgccgag cgcgaacctc gagctcgaac tcaccgagtc gctgctgatc
     8101 caggactcgc tgcagctggc cgagagcctg gcgagcctgc ggcgcatggg cgtgcgcttc
     8161 gcgatcgacg acttcgggac ggggtactcg aaccttgcct acctgaagcg cttcgaggtg
     8221 gagcgcctga agatcgacca gaccttcgtg cggcggctga ccgacgacgt gcaggaccag
     8281 gccatcgttc gcgcgatcgt gcagatggcg agcagcctga acctctacac ggtggccgaa
     8341 ggggtcgagg acggggcgac gctggcgctg ctgggggatc tcgggtgcca gcagggacag
     8401 ggcttccatt ggtcgcctgc ggtgccggcg gcggacttcg aggcgttcct gcgttcgtag
     8461 gcccgggccc cgctgacctc cgggtctggc cctgcctcga atcgcgcgaa tcgcagtccc
     8521 tgccatcgcc gcgattcgag gccgggcctg acaccgattc gccgttgcgt cccgcttcct
     8581 tgctgtctca gagattgccc gtcacccgga tcagcgtgtc gtcctgctgc gtgttgagca
     8641 ggtacagatc gcgtgtcgcc ggatccgcgg tcgacgcggc gttggtcgcc tcgacgaagc
     8701 cggtgtgccc cgaatcgcgg aacgtgccgg tcagccacgc ggcgttgctc gcggggagcc
     8761 ggccgatcgt cgcgcccgcg cggttggtcg cggtgtcgat cgcgacgacg aggccgccgc
     8821 tgacgccgtc gacggtgtag gcgtagccgt tggccgggtc ggtcacggtg acgggcgtga
     8881 ggttctgcac ctggaggacg gtctccagcg gcgtggcggt ggtgacggtg tcgtcgggcc
     8941 agggttgctg ctcgccgccg ccggcgaagc tggagttggc catcggggcc tggatcacgc
     9001 tgccgtccag gccgacgatg ccgctggcgg tctggctgcg cacgacggtc ctggcgctcg
     9061 cgttcgtgac gctggaccag gtcgtgtagt agaccgtcgt cgccgtcgcg gtgaagctgc
     9121 cgccgttgac gctgctcgcg accacggtga agggactgcc gcccgactgg gccagcgcgc
     9181 gcaccgtgta gagcggcgtg gtctcgctcc acacgaggtt ggtgccgccc accgggaacg
     9241 cgaggctgtc caggtgcgcg gcctcggtgg cgaccaccgt gccggccgcg gagccgtcgg
     9301 cggggatcgc gacgatgctg gccgatgccg gcgtgctgcc cgcggcgggc gtgttcacgc
     9361 cgacgtacag cgtcgtgggc gaggccgcga acagcgcgcc gttgttggtg ggcgtccagt
     9421 tggggatcgt gaacagcggc ggcgaggtcg tcgccgcggc gtagtccacg cgcacgatgt
     9481 tgccgtcgac gatgaacagc tggcccgtcg gatagccctg cgtcgtgccc accggcagcg
     9541 cgagcgccac gccgatggcg ctggcgaacg tgcccagcac cacgggattg gcgaagttgt
     9601 tgtcgacgcg cacgagctgc gcgccctgct tgacgacgaa gcccgtgatg ctgccggtgg
     9661 ccgagcgcac ggtggccacg ggcatcgccg acacggtgat cggcgcatcg gtcgaggaca
     9721 tgccggtgcg caccatgtgg atgacgtcgt cgccggtgtt gcagttgccg tccaggccgg
     9781 gcaggcgata gaagtacgcc gagttcgtcg gatcctgcag gtcggaggcg aagtagacgc
     9841 ccgcgtagtc gacggacgcg cccgctacct gggtgccggt cagcgagcat gtcgcgtcga
     9901 cggtggccgc ggtctcgttg gagatctgct ggctgtgcgg ctcgaagaag gacgcgaggt
     9961 cgatcgcgcg gatgtggccg tcggaggtct tccagatctc gacgccggcc ggctgcatca
    10021 ggtcctggcc ggtcaggaag tcgatcgtgc cgtgcagcga ctggcgcttg cccgcgtagg
    10081 tgcccatgtc ggcagccgcg tactcgcccg tggccgaatc ggcccaggcc gcgaacacgc
    10141 cggtggtgcc gacgtagctg atcagctcgc cgccgccatc gccgccgtca tacccgtcgt
    10201 cgtatccgcc gccgcagccg gcaagaaggg tggtggccgc gcccagcgcg atccaggacg
    10261 tgattgcccg catgatcgtc tcctcgttgt cgtcgagtcc aagcgattgg cctgctgccg
    10321 ggctttcgcg cacggcgcgg acatcgctcc tcgtgcgttg ttcaacgacg cggggccggg
    10381 cgccgatgac cggtcccgac tgagcgcgac ctgaaatttg tcaggggctt gtaaagcggg
    10441 cagcccgtgg cgacagacgc tctcgcgtgc ccgcgtgacg cgctcccgcg cgcgcggggg
    10501 aacgaggtgc gagcctgtcc ggaaatgcaa ttcaccaact cgaccggggt gaggacatcg
    10561 ctcctcgcac gccattaaac gtgctttcga tgacggtcga tgaccggggc cgggtgacaa
    10621 atgttcgatt gggtaagacc tcgcgccgtc gagcggcgcg ggcgcgccgc ctgcctgtca
    10681 gcgcccgagc gcgtcgcgca ggcgatcgag cgcgagggcc aggcgcgcga gctcgaaatg
    10741 ctgcggatcg tcgtgcgccc ccgcgatgcc ttggtcgagc gcggcatgcg tcgcgggaat
    10801 cagccagtcg ccgcggcgcg cgctcgccgc cgcgtagagc agcgccgcct gcgcatattg
    10861 cgcgtcggtg aagcccggcg tcggcgcgat cgcgtcgttg cccggcgccc cggccggatc
    10921 gcggcggcgc ggctgcgcga gttcgacgtg caggaacagg cccttcgcgc gcgggccgat
    10981 gcggcgcatc tccagttgcg tcgcgcgcca cgggacatcg aagccgtggc ccgtcagcgt
    11041 ctcgcctttc cggttcacga acacatgcgc cacggcctcg ggtcccgcga agcgggccag
    11101 tgcgttcagc gacgggtcgt cgtcgggcgg gaacgccgcg gcgtcgccca gccagggcgt
    11161 gctggtgtcg tgcaggacga aatagcgcgc ggccggcgcg gccgcgtcgc cgtcgcgggc
    11221 gcgcgagagc ggcgcggcca gcgggccgcc gatcgccgcg tcggggatgg catgcgccgc
    11281 gaggtagcgc tgcaggcgcg gtcccaggtc accgaccggc tggccgacca gcgaggcgag
    11341 cggatccggc agcaccgcgg ggctcgtcga gaccacgccg ccctgcgcca cgggccgcaa
    11401 gaggcagcgc gcctgcgcca gcgcatcgcc ggcgaactgc agcgtctcga cgtcgaaccc
    11461 gcacggtccg atcggccgac cgcccagccg cgtgagcgcc gcgcagcccg ccagcgcgag
    11521 gcctgcagcc gcgacggcca ggcccgcgaa cgcgatgcgt gtgcggatca tgcgggcatt
    11581 gtcgcccgcg cccttcagcg cgctgcgacc gccttggcgg gcgccttggc gggcggcttc
    11641 cagccgtcgg tgagcgtgcg caggaaggcg acgatgtcgt cgatctcgcg gtcgtccagg
    11701 cgtgggcggc cgccaggcaa cggctcgaat ggcacctcgt gcgagacgtt gccccagtac
    11761 tggcggggca ggtcgtcgta gccgcgctgg ccgtgcgaac cggggtacca gcgcgccggg
    11821 tccgtgtcgc gcgtggcgta gaaggcgacg gcgtcgcgca gcgtgcggat cgcgccgttg
    11881 tggaagaagc gccggcgcag cgccacgttg cgcagcgagg gggtgcggaa catgccgcag
    11941 tactccgggt gcgccgcgag gtcggtgcgc agcggaccgc acaggccgag gtcgaagaac
    12001 gtcgggtcgc ggttggcggg cagctcgcgg ttgcgcggca cgccgatggc cacgaagccg
    12061 aagtccgtga agatcggcgg acggttcgcg gcggccttgc gcgtgctcgg atggcagctc
    12121 gcgcagttgc ccttcttcat gtcgttgaac agcgccatgc cgcgcgcctc ggccgggctc
    12181 agcgtcgcct tgtcgttgag ccatgcgtcg aacttgctgt cgaacggatg gaactcgggc
    12241 gccgactgct cgtaggtctc gagcgccacc gtcacccata ccagcgtcga gtcggggtcg
    12301 tcgaacacgt tctcgccggg cgcgctcagc acctggcgga actcgtcggc gtacgacgtc
    12361 gtcttcagcc gcttgacgac ggcggcgccg tcggcgttgg ccatctcgtt gggatccatc
    12421 agcggcatca gcgcctggtc gtgcgcggtg tcgacgcggc cgtcccaggt gcgcccgccg
    12481 gtggggccct cgtcatcgac gcccagcgtc acctgcgact cgaggaagtg ctgcgtgaat
    12541 gcgaccggcg cgtgcacgta gcgcagcgac ggcgtgttgc gaaagcccgt cctgtccagg
    12601 tgcgggccgc ccggctgcac ggcgcggtcg ttgggcgggc cgaaggccgt ggccgggtcg
    12661 tggcagctcg cacacgactg cttgcccgag gccgagtacg ccggatccat gaagatgcgg
    12721 cggccgaggt cgctcagctg cgcgacggtc ggccggtcgc cgtacatcag cgggaagtgg
    12781 gtctcggtgc ccgaaggctc gggcgcgttg cccgagcagg cctgcgcgag acgcgcgatc
    12841 ggcagcaggc tcgcgaccgc cacgacggcg atcgcgagcg cgccgagtgc gcgcggcggc
    12901 ctcgttcgca tcgcgtcagc ggcgacggcg cgcgaggccc agcgacgtca gcagcagcat
    12961 gccggccagc acctgcagcg cgcccgccgg ctccggcacc accgacacct gcaacccgat
    13021 ggaggccgcg ctcgcgtcgt acgtgaagcc ccagtcggac gtggtggccg tggacaccgg
    13081 gcgcgcgacg ccggtgtcca gcagcgcgcc ggtggcggtc ggcgtggtgc cgagcgtgtc
    13141 gttcccgttc acctcgaact cgatccagta gctgccgggc gcgacggtcc aggccaggcc
    13201 cgacgcgcca ttccagccgt cggccccgta ggtggccgtc gtggtgaaca gctgcgcgcc
    13261 cggcctggcg ccgccgtcgt acagcgagat gtcgaaggtc tcgccgctgg tgccgccgag
    13321 gatgtggccg gcgatggcgg cgatcgtcga ggcgttggcg aagctgacct gggcggcggc
    13381 ccagtcgctg ccgtcgaaca cgagcgagcc gacgggcgag ccgctcgggg cgccggtgtc
    13441 gacgacggtg gcggcctggg cgagcggcgc gagcgccagc agcgcggcgg tgatgagcgt
    13501 cttgtccatg gcgcggctca cggggcgatg aagtactggc ccagcagctg ggtcgggccg
    13561 aaggtggaac tgaagccgta gccgaggatg tcgacgccgc cgtcggcgtg caccttgacc
    13621 gtggcccagg cgtagtcgcg gtcggtggcc ggatgcgcca gcacgccggc cgtggcctgc
    13681 gcggcgctcg ggtcgaacgg cgagccgccc gcgcccacga tcacctggta cgcgccgccc
    13741 ttgggctgcg agatgttgta cgtgtgcatg tggccggtga agtacgtcgc cttgtattgc
    13801 tcgatgacgt tccagaacgc atcgcgcctg gcggtcgtcg tcgccgtcgc gtccaggccg
    13861 gagcccgaca cgcccggggc gtatgcgtac gtgaacgccg gcttgtggcc gaacacgaag
    13921 aagcgcttga cgccgcggcc ctgggcggcg gcgaggtcgg cggccagcca cgcggtcggg
    13981 gccgaggtct cggcgcccac cgcatcggtg ttgatgacgg cgaagtgcag gccctggtag
    14041 tcgaacgagt acgacagctt ggactggtcg gtcggcagcg cgtcggggcc gacgccggcg
    14101 gccgggccgt agttcacgtt ggccggcgcg gcgcccagca cgctcgtgaa gcgcgcggtg
    14161 tcgaggatca ggtcgcccat gttggcggcg tacgcctgct cgttctccac cttggcctgc
    14221 gtctggccgt cgctgttctt cccgcaggcc ttgcactcgg tctcgtggtt gcccggcacc
    14281 gggaacacgt aggtgccggt ctcgggcagc ggcgccacca ggccgcgcca gaacgcgtac
    14341 tggcggtatt ccttcatcag gtcggagccg acgatgtcgg acgtcgtcgc gggcgccacg
    14401 gggccggcga tcgcgctctg cgtggcgttg gacgtgtagc cgtagttggc ccagccgtag
    14461 ccgtggatca tgtcgccgtt gaagaacagc atcgtcgact tctgcgactg gatcgtgcgc
    14521 aggatgcgcg tgaacgcctt ggtgttctgc agccaggtcg cgtcctggcc ggacagcgtg
    14581 ggcccgacgc tcgcctggtc gtacttgacc ggatcctggc gcgaatcgcc caccgtggag
    14641 aacgtgagga cgaccggcgt cgtgtcctgg gcgtgggcgg cgggggccgc cacggcggcg
    14701 gcgatcgcca gcagcgcggc attcctggct gtgtggtgcg tcatggtcat cctgctcttt
    14761 gtgttggcct cgggggagtt cgcgaggtgg caggattgtc gaaagcgtcc gtgacggtcg
    14821 cgtgacgcac gggggcgcgc gcctcggggg aagacccgcg gcttgcttgc cgaggcgggc
    14881 acgcgcgcgc cgatcggcgc caaggccgct cagtcgatcg gcggggcgcc gggcgcggcg
    14941 acgggcgccg gcggcggcac ggcgcagccg gcgcggtgct cggcccagta cgccacgttg
    15001 ccgatgatgg cgatcgagcc cgagacgatc gcgctgaccg ccgtcacgcc caggctcgcg
    15061 agcaccaccg cctcgcagcc ctgcccgctg cacgaattga gctggcccag caccaccgac
    15121 tgcagctcga tgtggtgcgt gacgagccgg caccccggat cgtagtcctc catctgcacg
    15181 ggcacgatca cgcagccggc gccggcgagg ccggcgacaa gcgcggcggc ggatcggcgg
    15241 cgcatcgctc gcggctcagg cgtcgggccg gatgcccagg gacggcgcga cgcaggcggc
    15301 cttgcgctcg gaccagtaca ccacgttgcc gaccaccacg accgatcccg agaccaccgt
    15361 gctgaccgtg gccaggccca cggcgccgag cacgcagaac ggctggaaga actgggtctg
    15421 gcccgaacaa cccaccacct ccttgagctg caccgacgtc agctccatgt ggtgcgtggc
    15481 gaccttgcag tcgtcgtcgt acgaatcccg ggtcatgggc acgacgacgc agccgctggc
    15541 cagcaggccg gcggcgaccg cggggacgat tcgtgtcagc ttcatgaagg gcagtatcgc
    15601 cgcttcactg tcgagccagc tgctctttac gccacgttga cggtgagtgg ccggtctcgc
    15661 gctcgaagaa gcgcgtgaag tagccggcgt cgctgaagcc cagcttgtag gcgatgtgct
    15721 tgacgctcag cgtggtgtac gcgagctcgc gctgcgcttc caggcacagg cgcgcgtgca
    15781 gcaccgccag cgcagggtgg cccagcacgc tgcggcagac gcgattgagc tgcgtcgtcg
    15841 tgacgccgat ctcggcggcg aactcgccga gcgtgggctg ctcgcggaag cgctcgtcga
    15901 ccagcgcgcg gaagcgctgc acgtgcacca ccgagcggtc gccgacgccg gccgccgccg
    15961 cgggcgccgg caggctgcgg ccgagcgcga cgaacagcgc cgtcatcgcc gcatcgatcg
    16021 cgctggcccg ccacggcgca tgctgcgtct gctcctcgtg cagcgcctcg acggccgtct
    16081 tgaccgcctt gcggtgcgcc ggcgcgaact gcacgcagcg cgagacgagc acccgctcgc
    16141 gcaggctctc gtcgcgcgcc agcagcgtgc gcacgtgcga ctcgcgcatc gtgaagacga
    16201 tgcccttgac gtcgctcgtc cagcggaagc cgtgcaccgc cagcggcatg accaggatga
    16261 cgcccggacc gcgcagcgaa tgcgcgtggc cgtcgatcag catctcgacc tggcccttct
    16321 cgaaccagaa gacctgcagc agcgcctcgt ggcggtgcgg cttgatctcc cacgaatgca
    16381 gcgcgctgcg ctcggcgatc gactccgagt gcatgacgtc ttccggcggc aggccggcgt
    16441 cctcgccgta caacaggtag gtgggcaggt gcaggggcat cgcaggttcg gattgctcaa
    16501 ccggccgccg cgccacgaaa cgctttcacg cgccaaccaa agcacggtgc gccgaatcca
    16561 cgggggccga tgttccgatt gttcaatcgt tcgccgattc tgtccattgg ctcgcccggg
    16621 cccgcgccgt acgcttcgct ccacgaattc cagccttccc cgggagatcc cgaatgcccg
    16681 cctcgacgcc ctgcatcatt tccgtcgcga tcaccggctc gctgccgcgc aagaaggaca
    16741 accccgccgt gccggtgacg gtgtccgagc aggtcgagtc gacgcatgcc gcctacgagg
    16801 ccggagccac gctggtgcac ctgcacgtgc gcaacgacga cgagacgccc acgtccgcgc
    16861 cggagcggtt cgcgcaggtg ctggacggca tccgcaagca ctgcccgctg atcatcacgc
    16921 aggtgtccac cggcggccgc tcgggcgccg gccgcgagcg cggcggcatg ctgcacctgc
    16981 gccccgacat ggcgtcgctg gccccgggct cggtcaactt cccgacccgc gtgtacgaca
    17041 acgcgcccga cctcgtcgac tggctcgcga gccagatgcg tgcgtacggc gtcaagcccg
    17101 agatcgaggc gttcgacctg tcgatgatct tccaggccgc ggcgatggcc agggatggcc
    17161 gcatcgaggg cccgctgcac gtgcagttcg tgatgggcgt caagaacgcg atgccggtcg
    17221 accgcgaggt gctcgagttc tacgtgaaga cgctgcatcg cctcgcgccg gacgccacgt
    17281 ggaccggcgc cggcatcggc cgcgaccagc tgacgctcgc gaagtggtcg ctggagctcg
    17341 gcggccattg ccgcaccggc ctggaagaca acgtgcgcct cgaccgcgag acgctggcgc
    17401 cttcgaacgc ggcgctggtc cggcagcttg ccgggctgtg cgagcaggcc ggtcgtcgcg
    17461 tggcgacgcc cgcggaggcc cgcgcgttgc tgcgcctggc gccgcagccc ggtgcgcgca
    17521 cggccgcgca gctccacgcc gagcacgtcg cgcatgcctg atccgcagcc tggaggagac
    17581 accatgcacg cgaccgctcc gctcgcccgc cccgacgacg aatacctgat gcgcgacccc
    17641 gcgtcgcatc cgcccgcgta cgcccccggc tacaagacga gcgccctgcg cagcccgcgc
    17701 aacgcgctga tctcgatcgg ccagacgctg tcggaggtca ccgcgcccgt gttccgtgcg
    17761 gaggaactcg gcccgctgga cggcgacctg atccgcaact acgccaagac gggcctgccg
    17821 atcggcgagc gcgccatcgt gcacggctat gtccgcgacc agctgggccg gccggtgcgc
    17881 aacgcgctgg tggaggtctg gcaggccaac gccagcggcc gctaccgcca caagaaggac
    17941 cagtacctcg gcgccctcga cccgaacttc ggcggctgcg gccgcgtgct gaccggcgac
    18001 gacggccact acgtgttccg cacgatcaag cccgggccct atccctggcg caaccgcatc
    18061 aacgactggc ggcccgcgca catccacttc gcgatctcgg gcagcggctg ggcgcagcgg
    18121 ctcgtcacgc agatgtactt cgagggcgac ccgctgatct cgacgtgccc catcatccgc
    18181 acgatcgcgg acgaggaaca gatccgcggc ctgatcgcgc agctcgacct ggccgccacc
    18241 gtgccgctgg acagccgcgc cttccgcttc gacatcgtgc tgcgcggcca gcgcgccacg
    18301 tggttcgaga acgtcctgca gtcggacggc caggcgagcc atgaaggagg tgtccgatga
    18361 ccccgctgct gcgcgagacc gcctcgcaga ccgccggccc gtacgtgcac atcggcctcg
    18421 cgcccagggc cgccggcttc gacatcttcg agaacgactt cggccccgtg ctgaccacgc
    18481 ccgggacccg gggcgagcgc atcacgatcg agggccgcgt gttcgacggc tcgggcacgc
    18541 cgctgcgcga cgtcctgctg gagctgtggc aggccaatgc gcaaggccgc tacgcgcatg
    18601 ccgcggacag gcagcccggc aaggcgctcg acgacgcctt ccgtggctgg ggccgcgcgt
    18661 gcagcgattt cgagacgggc gtgtggtcgt tcgagacgat caagccgggt gcggtcgccg
    18721 gccgccacgg cgtcgccatg gcgccgcacg tgagcctgtg gatcgtggcg cgcggcatca
    18781 acatcggcct gcacacgcgc atgtatttcg acgacgaggc gcaggccaac gccgccgacc
    18841 cggtgctcaa cctggtcgag tgggaggtgc ggcgcaagac cctggtcggc aggcgcgagc
    18901 agcgcgacgg caggacgatc taccgcttcg acatccacct gcagggcgat gacgagacgg
    18961 tgttcttcga cgtataaacc gccgatgatc ttcgaccgct tcctcgcctc gcccgaggtg
    19021 atgcaggtgc tggacgaacg cgccttcgtg caggccatgc tcgacttcga ggccgccctc
    19081 gcgcgcgcgc aggcggccgc gggcgcgatc ccgccaggcg ccggcgaggc catcgccgca
    19141 gcgtgcgacg cgcgcgggtt cgacgtcgac gcgatcgtcg ccgagggcgc gcgcgtgggc
    19201 accatcgcca tcccgctggt caaggcgctg acggcggccg tcgcgaaggc cgacgccgat
    19261 gcggcgctgg tggtgcactg gggcggcacc acgcaggacg tgctcgacac cgcgcaggtg
    19321 ctcgtcacgc gccgcgcgct ggcgttgatc gaccgcgacc tgtcgacgct ggtcgacgcg
    19381 ttgctcaggc tcgcgaagac gcatggcgat gcgccgctgc tgggccgcac gctgatgcag
    19441 ccggcgcagg tgatcagctt cggcttcaag ctggcggcct gggtcgcgcc gttggtgcgc
    19501 gcgcaggaac ggctccgcct ggctggcgat tcggcgctgc aactgcagct cgggggggcg
    19561 gtcggcaccc gcgcggcgct cggcgagcac ggcgacgccg tcgcggccgg catggcgcgg
    19621 ctgctggacc tgcgcctgcc gccggccgcc tggcacacgc agcgcgacga gctcgtcgcg
    19681 ctcggttgcg agctgggcgt gcttgcaggc tcgctgggca agatcggcaa ggacatcgcg
    19741 ctgatggccc agggcgaggt cggcgagatc gcggagccct cgggcggcgg ccgcggcggt
    19801 tcgtcggcga tgccgcacaa gcgcaacccg gtcgccgcga tgaacgcgct ggccggcgcc
    19861 gtgcgcgccc cccagcgcgt cgcggcgctg ctggccgcga tgccgcagga acacgagcgc
    19921 ggactgggca actggcaggc ggagcccgcc gagttcggcg ccctgctggc cggcgttcac
    19981 ggggcgctcg cgtcgctcgc cttcgccgcg ggcgggctgc aggtgtctgc gcagcgcatg
    20041 cgcgcgaaca tcgacgcgtt gcaaggcctg gtgttcgcgg aggcgctgtc catgcgcgtc
    20101 gcgcgcgaga tcggcaagtc cgaggcgcac cactgggtcg agggcctggc cggggacgtc
    20161 gtcgccggcg gcgcgcagtt gcacgacgtc gcgctcgccg cgctcgcgcg ccacgcgcgc
    20221 ctgcgcgaac gcatctccgc cggcgagctc gccgccctct tcgaccccga cgctcccgcg
    20281 cgccacgcga gcgcgcttgc tgcgccgcag ctggccgcgc tcgccacgca cgccgaggag
    20341 ctgcggcgcg atccgccatg gcgggaccat cttcaaggaa gccaccatgc ctgatcgcga
    20401 ggacgacttc gaccgcggcc tgaagaaccg ccgcgccgtg ctgggcgacg cgtgggtcga
    20461 caagtcgctt gacggcgcca acgcgttcaa cgccgacttc cagtcgctga tcacgcgcta
    20521 cgcctggaac gacatctggg gccgccccgg actcgaccac acgacccgcc gcctgctggt
    20581 gctgggcatg acgatgggcc tggcgcgctg ggaggagttc gagctccacg ccggcgccgc
    20641 catccgcgcc ggcgtgccgc tcgagaagat ccaggaaacg ctgatgcagg gcgcgatcta
    20701 ctgcggcgtg ccggcggcca acaccgcgtt caagatcatg atcgaccagc tccgtgagca
    20761 cggcctgctg ccgggccccg acccgctcgg cgccggcacg cgcgtggcca cgcaccacac
    20821 gttcagcgtg ccgcagctga aggtggcgct gcagggccgc ggcgcgcccg tcgtgctcag
    20881 ccatgcgctg gggctggaca tggccatgtg ggacgatctc gccgcgcgcc tggccgcggg
    20941 cgggctcggg gcgccgcgcg aagtcctgcg cttcgaccac cgcggccacg gcggctccgc
    21001 cgcgccggcc ggtccgtatg cgatggacga gctcgtcgac gacgccgcgc gcctcgtgcg
    21061 cgagtggggt cgcgggccgg tcacgtgggt cgggctgtcg atgggcgcga tggtgggcat
    21121 ggggctggcg atccggcatc cggagctggt cgaccggctc gtgctcgcga acaccacgtc
    21181 gcgatacccc gacgccgcgc agtcggcctg gtcgcagcgc atcgccaccg tcaatgcgag
    21241 cggcatggcg gccgtcgccg agatggtggt cgagcgctac ctgcacgccg acttccgcgc
    21301 cgcgcgtccc gaggccacgc aggccgtacg cgccacgatc ctgcgcaacg accccgccgc
    21361 ctacgccgcg agctgcgcgg ccgtggccgg cgtggcctgg caggatcgcc tcgccaccgt
    21421 ccgctgcccg acgctggtca tctccggcgc ccgcgacgcc ggcgccccgc ccgcgatggg
    21481 ccaggcgatc gcggagcgag tgccgggcgc gcgcttggag gtgatcgccg atgcgtcgca
    21541 cctgagcgtg ctggagacgc cggaggcgtt cgaggcgctg cttcggccgt tcctgtaatc
    21601 caaccctgcg tgtcatcctg cgcgcagtcg caggatgacc atcccggggt caggtcttga
    21661 acgttgcggg gtcaggtctt gaaagttgcc cggccaaact ttcgctctcg tgaagtgctc
    21721 gagcgcgcaa ctttcaagac cggaccccat cttggaccac gcccgcgcgc aacgattcaa
    21781 gacctgaccc cgcaacgttc aagacctgac cccggaatcg gaatggaaaa acgccgccca
    21841 cgaaggcggc gcaagagacg tcaggcgccc gaccgcgcgc cgccgccacc agacatcact
    21901 gcttgacgac gaacaccacc gccgtgcggg cggggatggt gaacgtgccg gacgccgtgt
    21961 cgaccgtcgc ctgcgcggcg cgcttgtcgg cggccgcgaa cccggcgtgc accgggtgca
    22021 gctggaacgc cttgccggcc agcgcgggga tggtgatggc gtgcgcggcc ttgtcgacgt
    22081 tcacgaagta cgtcagctcc tggaagttgg cgccggcgta gccgcggccg tcgaggtcgc
    22141 cgaccagcag cgtcgggatc tgccgcgagc ccgtgttgaa gaacttcagg cgtgccttga
    22201 tgtcgtcggc gctggccatg cgcagcagcg tcgagctgcg tcggatcttg agcaggtcgc
    22261 ggaactcgtc gcgcgcccac gcgatctcga ccggcgtcgg cttgatgtcc gcgttggcga
    22321 gcagcggctg ctgcatggtc cagttggact ggttcgagcc ggcgggcggc aggccgacgc
    22381 cgaagttgtc gtcctggtag ctccagtcga ggcgattgaa ccaatcgccc gagtcgtagc
    22441 tgttgccgtc cagggacttg ctgcgcagcg tgtccacgcc cgcatggaaa taggccacgc
    22501 cctggctgaa cgcgttgatc gcggcgccca gcatctgcac gcgcgcccgg tcctcgcgcg
    22561 acgtggtcgt cggcagcttg tagacgttgt tgtcgaagat cgtcaggttg tcgtggttct
    22621 cgaagtagtt caccacctcc gacggattcg tcacgtagcc ggcgggcagg ccctcgaagc
    22681 tgatctgctc cagcggcagc tgcgcatccc aactcgtggt cagcgagtag ctgcggatcg
    22741 agccggccag gctggccttg atcacgtcgc ccagccacat caggtcgccg gcgccgtggc
    22801 cgccggcctg cgggttcggg tcgtaccaca ggccgttgat gtagccctgg ttggacatca
    22861 gcgcgccgcc ggagtcgcag cagccgccgc cgcggatcgc gtcgcggatg gccgggttga
    22921 aggtgccgat gcccgtgccg ttcaggtcga gcatgtcggc ctgcacgaag cgcgcgccgt
    22981 tggccaccgc gccgaagttc caaccctcgc cgatcagctc gacgtggtgg cccacggccg
    23041 cgtcgacctt cgcctgcagc gccaccatcg tgtcgcgcgg ctgcaggccc atgatgtcga
    23101 agcggaacga gctcacgtgg tagtcgcgcg cccaggtgac gaccgagtcg gtcatcaggc
    23161 ggcccatcat catgttctcg gtggccgtgt cgtcgcagca cgagtcgcgc agcacgttgc
    23221 cgttggcgtc gaggcgatgg tagtagcccg gcacgacccg gtccagcacc gagtggtcgt
    23281 tctggcccga cgccgtcgtg tggttgaaga cgacgtccat gcccacgcgc aggcccgcct
    23341 ggttgagcga ctgcaccatg cggcggaact cgaccacgcg cgtcgagccg tcggccaccg
    23401 acgtggcgta gctgccttcc ggcgcggtga agtggaacgg gtcgtagccc cagttgtagc
    23461 agtcggtcga ggccgtggca gcgacg
//
LOCUS       DATCRO010000746         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_717292, whole genome shotgun sequence.
ACCESSION   DATCRO010000746 DATCRO010000000
VERSION     DATCRO010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717292"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..423)
                     /locus_tag="VIN75_20300"
     CDS             complement(118..423)
                     /locus_tag="VIN75_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY8976567.1"
                     /translation="MSKHCTHTHLIATVTPSARGCEECLKTGDEWVHLRLCRICGHVG
                     CCDDSPNRHATKHFHATGHPIIEGYDPPEGWGWCFVDRLMLELPDQTPQDGPIPRFF"
     gene            complement(592..1497)
                     /locus_tag="VIN75_20305"
     CDS             complement(592..1497)
                     /locus_tag="VIN75_20305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8976568.1"
                     /translation="MDRHAHLLDPPTEDQRPRDGATTRVRTVWISDLHLGTPGCQAVA
                     LLDFLKQVDCDQLFLVGDIIDGWQLRRSWYWPQAHNDVIQKLLRKARKGTRVIFVPGN
                     HDEFARKYVEHEFGGIEVHGEWMHVTADGRRLWITHGDLFDGVIQCAKWLAYVGDTLY
                     TVILRLNKHFNSMRARMGLPYWSLSKYLKHKVKRAVSYIGEFEVAVAREARRRGADGV
                     VCGHIHHAEMRDIDGVLYCNDGDWVESLTALVEHADGRLEILDWSTRGRPGVRTGPDD
                     EVPAAARPGVREPELALMSEVLAAR"
     gene            1634..2041
                     /locus_tag="VIN75_20310"
     CDS             1634..2041
                     /locus_tag="VIN75_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8976569.1"
                     /translation="MNHKPVLAHDDVVRILDHARSEAQSNQWAVTIAIVDDGGHPLAL
                     ERLDGASPASAYIATEKARTAALGRRPSAAYEEMINGGRTAFLSAPLAGMIEGGVPVI
                     VAGHVVGAVGVSGVKSAQDAQIAGAGIAALGAA"
BASE COUNT          319 a          813 c          823 g          337 t
ORIGIN      
        1 gtggtcgacg agccgtgggc ggagggcgtg gcggcgagcg tgccggtggt ggtcgcgggc
       61 gattgaggag cgcgtggacg aggggccggc cccctccgac cgcgcgcacc gtccaggtca
      121 gaagaacctc gggatcggcc cgtcctgcgg cgtctggtcc ggcagttcca gcatcagcct
      181 gtccacgaag caccagcccc acccttcggg cgggtcgtag ccctcgatga tcggatggcc
      241 ggtcgcgtgg aagtgcttcg tcgcgtgccg gttgggcgag tcgtcgcagc acccgacgtg
      301 gccgcagatg cggcacaggc gcaggtgcac ccactcgtca ccggtcttca ggcattcctc
      361 gcagccgcgc gcgctcggcg tcacggtcgc gatcaggtgg gtgtgggtgc agtgcttgct
      421 catgggtggc ctccggtcac gacggggaac cgcccgattc tgcgcgcatc gcgtcgcgtg
      481 cgccggacgc cttcagcgac ccgtcccggc caggtcggcc gtgcgaacac gccgtcgacg
      541 acgagcgcct tgcgcaccga cttcggatcc gccgtatcga tgcggtcgct ttcaacgcgc
      601 cgccagcacc tcggacatca gcgccagctc gggctcgcgc acgcccggcc gcgccgccgc
      661 cggcacctcg tcgtcgggcc ccgtgcgcac gccgggccgg ccgcgcgtcg accagtcgag
      721 gatctcgaga cggccgtccg cgtgctcgac cagcgccgtc aggctctcca cccagtcgcc
      781 gtcgttgcag tacagcacgc cgtcgatgtc gcgcatctcc gcgtggtgga tgtggccgca
      841 gaccacgccg tcggcgccgc gcctgcgcgc ctcgcgcgcg accgcgacct cgaactcgcc
      901 gatgtagctg accgcgcgct tgaccttgtg cttgaggtac ttcgacaggc tccagtacgg
      961 cagccccatg cgtgcgcgca tcgagttgaa gtgcttgttg agcctgagga tgaccgtgta
     1021 gagcgtgtcg ccgacgtagg ccagccactt ggcgcactgg atcacgccgt cgaacaggtc
     1081 gccatgggtg atccacaggc gcctgccgtc ggcggtgacg tgcatccact cgccgtgcac
     1141 ctcgatgccg ccgaactcgt gctcgacgta cttgcgcgcg aactcgtcgt ggttgcccgg
     1201 cacgaagatc acgcgcgtgc ccttgcgcgc cttgcgcagc agcttctgga tgacgtcgtt
     1261 gtgcgcctgc ggccagtacc acgaccggcg cagctgccag ccgtcgatga tgtcgcccac
     1321 caggaacagc tggtcgcagt cgacctgctt gaggaagtcc agcagcgcca ccgcctggca
     1381 gccgggcgtg cccaggtgca ggtcggagat ccagaccgtg cgcacgcgcg tcgtcgcgcc
     1441 gtcgcgcggc cgctggtctt cggtcggcgg gtccaggagg tgggcatggc gatccatgga
     1501 cgcccatcgt gcgccgcgcg cgtgatgctg ccgcgacagc gacgtgacgg ttgcatgact
     1561 cggggccaga gtccgaccga ctgcatgggc ccctgcgcag tgctacaacg cggacttccc
     1621 tgaaaggccc ctcatgaatc acaagcccgt cctcgcgcac gacgacgtcg tccgcatcct
     1681 cgaccatgcg cgcagcgagg cgcagtccaa ccagtgggcg gtgacgatcg cgatcgtcga
     1741 cgacggcggc catccgctgg cgctcgaacg cctggacggc gcgtccccgg cgtcggccta
     1801 catcgccacc gagaaggcgc gcaccgcggc gctcgggcgg cggccgtcgg cggcctacga
     1861 ggaaatgatc aacggcggcc gcacggcctt cctgtcggcg ccgctggccg ggatgatcga
     1921 gggcggcgtg cccgtgatcg tcgcgggcca cgtggtcggc gcggtcgggg tgtcgggcgt
     1981 caagtcggcg caggacgcgc agatcgccgg cgcgggcatc gcggccctcg gcgcggcctg
     2041 acccgaggcc ggacgaagaa aagcccgccg cggccaggcc ggggcgggcg aaaaggcgct
     2101 ggacggcctg cccgagacgg gccgccccgc gtcctgtctc agaacgagtg gcgcacgccg
     2161 aggacgaact cggtgaccga gcggtcggtc gcggtcgtca ggcccgcggg cgtcgagccg
     2221 ccgatgcggc ccacgaacgt gccgctggcg ctggcatcgt tctggaagcg cgtcagcgac
     2281 gtgtacaggt cg
//
LOCUS       DATCRO010000747         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_548900, whole genome shotgun sequence.
ACCESSION   DATCRO010000747 DATCRO010000000
VERSION     DATCRO010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548900"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VIN75_20315"
     CDS             complement(<1..997)
                     /locus_tag="VIN75_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8976570.1"
                     /translation="MGTSIFVSVCRQSLPVIVASLAVASANAQPSSATGPQVMGWVPA
                     YGIDASMKTLAATPAIGQAMTRIGLQFWNPSNDGRGLVLAPVDATGKPVTAANVRQVA
                     QWARAHNVQPLLTVYNNSQVINRWDWQWARRAFAEHPEEFAAALVAAVDKWDLDGVDL
                     DLEGEGDFAADRAAYAAFVHQLAAALKAKGKLLTVDSFHSPCDNAPNMRWWSDWVGDV
                     ATIHSMGYEDLYEGSNATFTPEGRPVCENGATLFRYSWQLDWGTKAGYRRDQIVLGMP
                     TWVDAWGSGGIGPNVVDHLREVRALGGGVGLWDMQLAAAGWSKPATWEAVQALRRP"
     gene            1209..2231
                     /locus_tag="VIN75_20320"
     CDS             1209..2231
                     /locus_tag="VIN75_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8976571.1"
                     /translation="MSTPRLVNLALQGGGAHGAFTWGVLDALLEDGRIALDGLSGTSA
                     GAMNAVVLAHGWLEGGRDGARAALRRFWTALADTMPLEVTRQDVGGGVHLAPLVRLWM
                     AWAHYVTPAQFNPLDVNPLRALLSGQVDFERLRRERPMRLFVAATDADSGRLRVFEGR
                     ELSVDALLASSCLPTVFHAPRIAGRTYWDGGYAGNPAVFPLLRGGRSLDTLLVLLSPL
                     EWAPMQPAASAPELRARVAEIAFTAAFRREIQALAQARGDRASRARRAWRRWLLGEPP
                     APRFHLIEGGPALEHVAAETRLAVSARFFEALHELGRETASRWLARHFDALGRRDSVD
                     LAATFG"
     gene            complement(2270..2683)
                     /locus_tag="VIN75_20325"
     CDS             complement(2270..2683)
                     /locus_tag="VIN75_20325"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY8976572.1"
                     /translation="MGLLDSIVGQVAGSLQGSVPQGQVHPGLLDVVSSLLTQGGGLQG
                     LINQFESQGLGNVVSSWIGTGQNLAITPEQVQSVLGDSHIAEVAAKLGLQPQDVANQL
                     AGLLPHAVDSVTPSGAVPQGNLLSEALNAFTALRR"
     gene            2978..>3894
                     /locus_tag="VIN75_20330"
     CDS             2978..>3894
                     /locus_tag="VIN75_20330"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8976573.1"
                     /translation="MNALKKLVKRLRANSRVPVAVLSVLLVAGGFVLLAQEATRETNA
                     RKSPEMLAFEQTPDAWLQNQRNVSQLVKAIDAGQVATVALANGHPGLVLYTLKNGAKA
                     SATVPGCTPIGCAGTALDKLAERSAAQGFALVGVDVDPRTKSQHLLDALGAIVSPLLV
                     ILTMGVGFFVLMRMQTGMGGGASTLSERPETKFADAIGNDEAKAALNRVKAFMSDPSA
                     YVKLGASAPRGVLLVGPPGTGKTLLARALAGESKANFIAVDGSYFTATYYGAGVAKVK
                     ALFKQARKHAPCVLFIDEVDGIGKRSRGN"
BASE COUNT          551 a         1379 c         1364 g          600 t
ORIGIN      
        1 tgggccgccg cagcgcctgg acggcttccc acgtcgcggg cttgctccag ccggccgcgg
       61 ccagctgcat gtcccacagg ccgacgccac cgcccagcgc gcgcacctcg cgcaggtggt
      121 cgaccacgtt cgggccgatg ccccccgagc cccaggcatc gacccaggtg ggcatgccca
      181 gcacgatctg gtcgcgccgg tagcccgcct tggtgcccca gtccagctgc cagctgtagc
      241 ggaacagcgt cgcgccgttc tcgcagacgg gccggccctc gggcgtgaag gtggcgttgc
      301 tgccttcgta gagatcctcg tagcccatgc tgtggatggt ggcgacgtcg cccacccagt
      361 cgctccacca gcgcatgttg ggcgcgttgt cgcacgggct gtggaagctg tcgaccgtca
      421 gcagcttgcc cttggccttg agcgccgcag ccagctggtg cacgaaggcg gcgtaggccg
      481 cgcggtcggc ggcgaagtcg ccttcgccct cgaggtcgag gtcgaccccg tccaggtccc
      541 acttgtcgac cgcggcgacg agggcggcag cgaattcctc gggatgctcg gcgaacgcgc
      601 ggcgggccca ttgccagtcc cagcggttga tcacctgcga gttgttgtag acggtcagca
      661 gcggctgcac gttgtgcgcg cgcgcccatt gggccacctg cctgacgttg gcggccgtca
      721 ccggcttgcc ggtggcgtcc accggcgcga gcacgaggcc gcggccgtcg ttgctcgggt
      781 tccagaactg caggccgatg cgcgtcatcg cctggccgat cgcgggcgtc gcggcgagcg
      841 tcttcatcga ggcgtcgatg ccataggcag gcacccagcc catgacctgg gggccggtcg
      901 cggaagaagg ttgggcgttc gcgctggcga cggccaggct ggcaacgatc accggcaagc
      961 tttgccggca gacggagacg aagatcgacg ttcccatgga ctttcttgca ccaacggcgg
     1021 gcgcagggta gcagatgtcg gatccgagca agaatcgggc tcagttataa gggttttcca
     1081 gctcgggtta acgagcgacg tgttgcgcaa cagggttccc ttgacaaagg tcgcggaaat
     1141 acccgaacaa aaccgcgggc acgcacgttc gcgcaagatg ctgcaatgca gcaagcaatt
     1201 gccgaccgat gtccacgccg cgtctcgtga acctcgcctt gcagggcggc ggtgcgcatg
     1261 gcgcattcac ctggggcgtg ctggacgcgc tgctggagga tggccgcatc gccctggacg
     1321 gcctgagcgg caccagcgcc ggcgcgatga acgccgtggt gctggcccac ggctggctcg
     1381 agggcggccg cgacggcgcg cgcgccgcgc tgcggcgctt ctggacggca ttggccgaca
     1441 ccatgccgct ggaggtgacg cgccaggacg tcggcggcgg cgtgcacctc gcgccgctcg
     1501 tgcgcttgtg gatggcgtgg gcgcactacg tgacgccggc gcagttcaac ccgctggacg
     1561 tcaatccgct gcgcgcgctg ctgtccgggc aggtcgactt cgagcgcctg cgccgcgagc
     1621 gcccgatgcg gctgttcgtg gccgccaccg atgcggactc cggccgcctg cgcgtgttcg
     1681 agggccgcga gctgagcgtc gacgcgttgc tcgcctcgag ctgcctgccc accgtgttcc
     1741 atgcgccgcg catcgccggc cgcacctact gggacggcgg ctacgccggc aatccggccg
     1801 tgttcccgct cctgcgcggc ggccgcagcc tggacacgct gctggtgctg ttgtcgcccc
     1861 tcgaatgggc gccgatgcag ccggcggcca gcgcgccgga actgcgcgcc cgcgtcgccg
     1921 agatcgcgtt caccgcggcg ttccgccgcg agattcaggc cctcgcgcag gcgcgcggcg
     1981 accgcgcgtc acgcgcgcgc cgcgcatggc ggcgctggct gctgggcgag ccgcccgcgc
     2041 cgcgcttcca cctaatcgag ggcggacccg cgctggagca cgtggccgcg gagacacggc
     2101 tggccgtcag cgcgcgcttc ttcgaggcgc tgcatgagct cggacgcgag accgcgtccc
     2161 gttggctcgc gcggcacttc gacgccctgg gccgccgcga cagcgtcgac ctggcggcga
     2221 ccttcggctg acagcggtgc ggcagggccg tccacgaacg ttcagcggtt cagcggcgca
     2281 gcgccgtgaa cgcgttcagc gcctcgctca acaagttgcc ctgcggcacc gcgcccgacg
     2341 gcgtcaccga gtcgaccgca tgcggcagca ggccggcaag ctggttggcc acgtcctgcg
     2401 gctgcagccc cagcttggcg gcgacctccg cgatgtgcga gtcgccgagc accgactgca
     2461 cctgctcggg ggtgatcgcc aggttttggc ccgtgccgat ccacgacgag acgacgttgc
     2521 cgagcccctg cgactcgaac tggttgatga ggccctgcag gccgccaccc tgcgtcagca
     2581 gcgacgagac gacgtccagc agccccggat gcacctgtcc ttgcgggacg ctgccctgca
     2641 gcgagcccgc gacctgtccg acgatcgaat ccagcaaacc catggtcgtt ctcctcgtgc
     2701 gttgtgacgc tcgtccagga acggacgagc cggctcaatg tagcgcgcgg cggccggtgc
     2761 gcgtgtgact tttcgtcggc gttccccgcg ccgggcgcca ctgcggtgat cgttcgcgct
     2821 gccgtggtcc agtagaaact tccacttgcg gttttccaca tcgatctggc gggtcgcagc
     2881 gtgccttttc cactggaact ttgggtcggc gcgcgctcca atggaggcac ggtatcgcca
     2941 cgtaccgcgg actgcttttg ccgcaagact tccccacatg aacgccctca agaaactggt
     3001 caagcgcctg cgcgccaact cccgtgtccc cgtcgccgtg ctgtcggtgc tgctcgtggc
     3061 cggcggcttc gtgctgctgg cgcaggaggc cacgcgcgag acgaacgcgc gcaagagccc
     3121 cgagatgctc gccttcgagc agacgcccga cgcgtggctg cagaaccagc gcaacgtgtc
     3181 gcagctggtc aaggcgatcg acgccggcca ggtggccacc gtcgcgctgg ccaacggcca
     3241 tccggggctg gtgctctaca cgctcaagaa cggcgccaag gccagcgcga ccgtgcccgg
     3301 ctgcacgccg atcggctgcg ccggtaccgc gctcgacaag ctcgccgagc gcagcgccgc
     3361 gcagggcttc gcgctggtgg gcgtggacgt cgatccgcgc accaagagcc agcacctgct
     3421 ggacgcgctg ggcgcgatcg tctcgccgct gttggtgatc ctgacgatgg gcgtgggttt
     3481 cttcgtgctg atgcgcatgc agaccggcat gggcggcggc gcgtcgacgc tgtcggagcg
     3541 tcccgagacc aagttcgccg acgccatcgg caacgacgaa gccaaggccg cgctcaaccg
     3601 cgtcaaggcc ttcatgtccg acccgtcggc ctacgtcaag ctgggcgcct ccgcgccgcg
     3661 cggcgtgctg ctggtgggcc cgccgggcac cggcaagacg ctgctggcgc gcgcgctggc
     3721 cggcgagagc aaggccaact tcatcgccgt cgacggctcg tacttcaccg ccacgtacta
     3781 cggcgcgggc gtcgccaagg tcaaggcgct gttcaagcag gcgcgcaagc acgcgccctg
     3841 cgtgctgttc atcgacgagg tggacggcat cggcaagcgc tcgcgcggca acga
//
LOCUS       DATCRO010000748         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_177251, whole genome shotgun sequence.
ACCESSION   DATCRO010000748 DATCRO010000000
VERSION     DATCRO010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177251"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VIN75_20335"
     CDS             <1..1153
                     /locus_tag="VIN75_20335"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEY8976574.1"
                     /translation="VQDSVAKAKVYSVTTGTGKRLVGVITLPSFYEDAAAMQKGDKDY
                     RSAARDVARLLADMKARKVAAVLIDLRNNGGGSLREAVNLTGLFVGKVPVLQTRNAKG
                     EITIEKNTETDLAWNGPMGVLINRASASASEIFAAAIQDYGRGVILGEGSFGKGTVQT
                     LASLDQIAKNATPAYGELKMTIAQFFRVNGGTTQLHGVTPDILFPQTGDDTQYGESSY
                     DNALPWTRIKAVDYAPVGDVQVQLPRLSAWHATRVEHDRDFQNLVQDVAQARELRKTN
                     VISLNEEVRRKERAASEKRVAAMLGAAATGASSALVDDGLQFNERQLDKDLAVEKTRK
                     TINDALLDEAVSILSDSAALKEGKSQGAASALPIRPEEVMTLPKGSSEK"
     gene            complement(1213..2781)
                     /locus_tag="VIN75_20340"
     CDS             complement(1213..2781)
                     /locus_tag="VIN75_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8976575.1"
                     /translation="MLSNIKIRTLIVAVLGLLMAAVVAIGALGYYSSWRTREAFRDVA
                     LRDRETETAFTKIRLLMELNRSQVLQALQHNPEFGWSKLHDHPLDVHWGAVDKASQDI
                     DALWKSYYAGVESADERQLADAWYAKSGGLGIASVRAAVAAIRGDRWDEAEQVLIKTI
                     NPTYRVGTSASSDLSAFLVKRGQANDASVEAETARLRWLLAAAVLSSFVLAALAMRAL
                     VRGITEPLQQAITIARRVADGDLGSRIEVDHGNELGDLLRSLKGMNESLVAIVGSVRD
                     SSDSIAIGAGQIATGNADLSHRTEQQAGSLQQTASSMEQLTTTVRANAEAALAADKLA
                     KSASDVAARGGATVMTAVATMDEINASSKKIADIIGVIDGIAFQTNILALNAAVEAAR
                     AGEHGRGFAVVAGEVRSLAQRSAQAAREIKTLIAASVERVEMGSRQVDEAGRTMEDIV
                     GQVKRVSDLIAEISTATHEQSGGLGQVGDAVTQLDRVTQQNAALVEQSAAAAESLKQQ
                     AARLVGAVAAFKVA"
     gene            complement(2835..3749)
                     /locus_tag="VIN75_20345"
     CDS             complement(2835..3749)
                     /locus_tag="VIN75_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976576.1"
                     /translation="MGSTDQFHSRAATELLQRLARGEVRQPARWTPADAARLLDRVQP
                     AASGHPGDQSRERGQLAAAFLAGVAQGHEETHAGPATSFVAMALQAARTLLKNRAATQ
                     ADRASHAFLAGLRVARAPAVPESPDEPASGGLRLFVRQPFTESGETQQGVVASVLERI
                     RALDGRPHAFEYLTGCRAESADTFRASFERETGHAFSPETFRAHRLRQLDRAGVFVNI
                     RAGMSESSAFELSYHVFRGACTPILFLVWKRAPIKTTLLKDLGSLCDVTYLEFDDPAE
                     LDGPLADFFGSCEQGRRSARPALARAGA"
     regulatory      complement(3867..3962)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4074..>4591)
                     /locus_tag="VIN75_20350"
     CDS             complement(4074..>4591)
                     /locus_tag="VIN75_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEY8976577.1"
                     /translation="QAMRLRDAHLPQGAPTSAALANLCAFRLDVRIAALARSLGARYT
                     RYADDIVLSGDAALLRAADRVEARIGAFALDEGFALNHRKTRRVTRARRQQVCNIVVN
                     ARPNLPRAEFDRLKAQLHLCATRGAAAQNRDGLPNWEQHLRGRVAWAEQLNPGKGARL
                     RRLLDAIDWTT"
BASE COUNT          671 a         1567 c         1674 g          679 t
ORIGIN      
        1 ggtgcaggac agcgtcgcca aggccaaggt ctattccgtg acgacgggca cgggcaagcg
       61 cctggtcggc gtgatcacgc tgccttcgtt ctacgaggat gctgccgcga tgcagaaggg
      121 cgacaaggac taccgcagcg cggcgcgcga cgtggcccgg ctgctggcgg acatgaaggc
      181 ccggaaagtg gctgccgtgc tgatcgacct gcgcaacaac ggcggcggct cgcttcgcga
      241 ggcggtgaac ctgacgggcc tgttcgtcgg caaggtgccg gtgctccaga cccgcaatgc
      301 caagggcgag atcaccatcg agaagaatac cgagaccgac ctggcctgga acgggccgat
      361 gggcgtgctg atcaacaggg cctcggcttc ggcgtcggag atcttcgccg cggcgatcca
      421 ggactacgga cgcggcgtga tcctcgggga aggcagcttc ggcaagggca cggtgcagac
      481 cctggccagc ctcgaccaga tcgccaagaa cgccacgccg gcgtacggcg aactgaagat
      541 gacgatcgcg cagttcttcc gggtgaacgg gggaacgacc caactccatg gcgtgacgcc
      601 ggacatcctc tttccgcaga ccggcgacga cacccagtac ggcgagtcga gctacgacaa
      661 cgccctgccc tggacccgga tcaaggcggt cgactacgca ccggtgggtg atgtgcaagt
      721 gcagttgccc cgcctgtcgg cgtggcacgc gacccgcgtc gagcatgacc gcgacttcca
      781 gaatctggtg caggacgtgg cccaggcacg ggaacttcgc aagaccaacg tgatttcact
      841 caacgaggag gttcgacgca aggagcgcgc cgcctcggag aagcgcgtgg cggcgatgct
      901 gggcgccgcc gcaacgggcg cgagcagcgc cctggtggac gacggcctgc aattcaacga
      961 acgccagctt gacaaggacc tggccgtcga aaagaccagg aagacgatca atgacgccct
     1021 tctcgacgag gctgtcagca ttctctcgga cagcgcagcc ttgaaggaag gcaagtccca
     1081 gggggcggcg agcgccttgc cgatccgccc cgaggaggtc atgaccttgc cgaagggttc
     1141 atcggagaaa taggtccgat gggccgaggc cggtcccggg ctcgcgacag cccgcgcggg
     1201 acggatcgcc cctcacgcca ccttgaacgc ggccaccgcg ccgaccaggc gcgcagcctg
     1261 ctgcttcagg ctttcggcgg cggccgcgga ctgttcgacc agcgccgcgt tctgctgcgt
     1321 cacgcgatca agttgcgtca ccgcatcgcc cacctggccg aggccgcccg actgctcgtg
     1381 ggtggccgtg ctgatctcgg cgatcaggtc gctcacgcgc ttgacctgtc cgacgatgtc
     1441 ctccatcgtg cggccggcct cgtcgacctg gcggctgccc atctcgacgc gctccacgct
     1501 ggcggcgatc agcgtcttga tctcgcgcgc ggcctgcgcg ctgcgctggg ccaggctgcg
     1561 cacctcgccg gccaccaccg cgaagccgcg gccatgctcg ccggcgcgcg ccgcctcgac
     1621 ggccgcattc agcgcgagga tgttggtctg gaacgcgatg ccgtcgatga cgccgatgat
     1681 gtcggcgatc ttcttggacg aggcgttgat ctcgtccatc gtggccaccg ccgtcatcac
     1741 cgtggcgccg ccgcgcgcgg cgacgtcgct ggccgacttg gcgagcttgt cggccgccag
     1801 cgcggcttcg gcgttggcgc gcacggtggt ggtcagctgc tccatcgacg aggcggtctg
     1861 ctgcaggctg ccggcctgct gctcggtgcg atgcgacagg tcggcgttgc cggtggcgat
     1921 ctggcccgcg ccgatcgcga tcgagtcgct gctgtcgcgc acgctgccga cgatggcgac
     1981 caggctctcg ttcatgccct tgagcgagcg cagcaggtca cccagttcgt tcccgtgatc
     2041 gacctcgatg cgcgagccga ggtcgccgtc ggcaacgcgg cgcgcgatgg tgatggcctg
     2101 ctgcagcggc tcggtgatgc cgcgcaccag cgcgcgcatc gccagcgcgg ccagcacgaa
     2161 cgacgagagc accgcagcgg ccagcagcca gcgcaggcgg gcggtctcgg cctcgacgga
     2221 cgcgtcgttg gcctggccgc gcttgaccag gaacgccgac aggtccgagg acgcgctcgt
     2281 gccgacgcgg taggtcgggt tgatcgtctt gatcagcacc tgttcagcct catcccagcg
     2341 gtcgccacgg atggcggcca ccgccgcgcg tacgctggcg atgcccaggc cgccgctctt
     2401 ggcgtaccag gcgtccgcca gctgcctctc gtcggccgac tcgacacccg cgtagtagga
     2461 cttccagagc gcatcgatgt cctgcgacgc cttgtcgacc gcaccccagt gcacgtcgag
     2521 cggatgatcg tggagcttgg accagccgaa ctccgggttg tgctgcagcg cctgcagcac
     2581 ctggcttcga ttgagttcca tcagcaggcg gatcttggtg aaggcggttt ccgtctcgcg
     2641 gtcgcgcagc gcgacgtcgc ggaaggcctc gcgcgtgcgc cacgagctgt agtagccgag
     2701 cgcgccgatg gcgacgacgg ccgccatgag cagcccgagg acggcgacga tgagcgtgcg
     2761 gatcttgatg ttggaaagca tggcgatggt ctcgttgaag gcggtcgatg cggggcgtcg
     2821 tgcgggctcg cgtgtcaggc gccggcgcgc gcgagggcgg gccgggcgct gcgccggccc
     2881 tgctcgcagc tgccgaagaa gtcggccagc ggcccgtcga gctcggccgg gtcgtcgaat
     2941 tcgaggtagg tcacgtcgca cagcgaaccc aggtccttga gcagcgtcgt cttgatcggc
     3001 gcgcgcttcc acaccaggaa caggatgggc gtgcaggcgc cgcggaacac gtggtacgac
     3061 agctcgaagg cgctgctctc gctcatgccg gcgcggatgt tgacgaacac gccggcccgg
     3121 tcgagctggc gcaggcgatg cgcgcggaag gtctcgggcg agaaggcgtg gcccgtctcg
     3181 cgctcgaagg aggcgcggaa cgtgtcggcg ctctcggccc ggcagccggt gaggtattcg
     3241 aacgcatgcg gccggccgtc gagcgcgcgg atgcgttcga gcaccgacgc gaccacgccc
     3301 tgctgcgtct cgcccgattc ggtgaagggc tggcgcacga acaggcgcag gccgccggat
     3361 gcgggctcgt cgggcgactc cggcacggcc ggcgcgcgcg cgacgcgcag gccggcaagg
     3421 aacgcgtgcg acgcgcgatc ggcctgcgtc gcggcgcggt tcttcagcag cgtgcgcgcg
     3481 gcctgcagcg ccatcgcgac gaacgaggtg gccgggcccg cgtgcgtctc ctcgtgcccc
     3541 tgggccacgc cggccaggaa cgcggcggcg agctggccgc gctcgcgcga ctggtcgccc
     3601 gggtggccgc tggcggcggg ctgcacgcga tcgagcagtc gcgcggcgtc cgccggcgtc
     3661 cagcgggccg gctggcgcac ctcgccgcgg gccaggcgct gcagcagctc ggtcgcggcc
     3721 cgtgagtgga actggtcggt ggatcccatg gtgtcgactc ccttgtcgtc tcatgccgag
     3781 gcagcggagt cgcagcagcg tccgtgggca gccggcgccc gcgcggggcg tgatccttca
     3841 cccggacagg cgtgacccgt ccggctcgcg gcgaccccgc tgtcgtggcc cgcatgacag
     3901 tgcgggcgtt agaccggaag ctttgcgtcc ccgcctttcg gtcgggtttg cctttgcggg
     3961 aagacgcttg tcgcgcatcc ctcgcgtcct gttatcggcg cccaccggaa caactcgata
     4021 cgtgtacggg gtatccgcat cagcaatcaa ggattcccga caggctgcgg ccgtcaggtc
     4081 gtccagtcga tcgcgtcgag cagccggcgc aggcgcgcgc ccttgcccgg attgagctgt
     4141 tccgcccagg cgacgcgccc gcgcaggtgc tgctcccagt tcggcaggcc gtcgcggttc
     4201 tgcgccgcgg cgccgcgcgt cgcgcacagg tgcagctgcg ccttgaggcg gtcgaactcc
     4261 gcgcgcggca ggttgggccg cgcgttgacg acgatgttgc acacctgctg gcggcgggcg
     4321 cgcgtgacgc ggcgcgtctt gcggtggttc agcgcgaagc cctcgtcgag cgcgaaggcg
     4381 ccgatgcgcg cctcgacgcg gtcggccgcg cgcaggaggg cggcgtcgcc ggacagcacg
     4441 atgtcgtccg cgtagcgcgt gtagcgggcg ccgagcgagc gcgcgagcgc cgcgatgcgc
     4501 acgtcgagcc ggaacgcgca caggttggcc agcgccgccg aggtgggcgc cccctgcggc
     4561 aggtgggcgt cgcgcagccg catcgcctgc g
//
LOCUS       DATCRO010000749         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_537662, whole genome shotgun sequence.
ACCESSION   DATCRO010000749 DATCRO010000000
VERSION     DATCRO010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537662"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIN75_20355"
     CDS             <1..324
                     /locus_tag="VIN75_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976578.1"
                     /translation="PELWFRWFHHAQPGGLESALLRRAAGQWAAFAIVQAIALVRWKA
                     QPVWLMVVAGLRASDLLTDLSYIAAVPSLTTLGWALLTPPAFLNAAFIITMVLAYRRL
                     TPGAR"
     gene            682..2346
                     /locus_tag="VIN75_20360"
     CDS             682..2346
                     /locus_tag="VIN75_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976579.1"
                     /translation="MAFMLRLYQEPDGKARRESGPTDSQVVTALRAVLQENFGSEPAD
                     YVSWALVRYGCRQKPTMSALHPWEGLLFEWQQRKASTPRIALMLRDAGFERPLTPQAL
                     EALDGWITDPVTALGAATDIIEALFGERLVSYNLHDDGSTPSHDVLFGGLVASLMPQA
                     KLALLRQSLVRQDDGAVWRVEYTYEGRERHFDVRADGSAMDVDGVMQGLDELMESIGR
                     VERAYRLAPGRYNNGETGTFVLVDSQRFPEIAWRLRLPLLRSPSVSDLPPGVPTAAPE
                     PLRVGARPMAPRPQPQAASTAREAVQDDVAVATRPGGGVTAPMSLLESQFSPPQPAAA
                     TTAAAEPSGPIERAADPAMKSLINNLRRSFGAGRLLQGGRWMKQVRTEPPAWLANGSD
                     PIRIFYEKQETLFRKGRIGWGALVEADDGVFARGEDDLPGVLVYSEDEYFDARPAELA
                     AIGARLVELKAAGAVGAFEPSRFAQSIRNDSGRPLGVTVPASLSAKEPMLSSFIAIRS
                     HLPDGLLAGDWFPVLIHPSSAVPMIVPCTFWPDAMVSAWNERRMSL"
BASE COUNT          320 a          869 c          901 g          374 t
ORIGIN      
        1 ccggagctct ggttccgctg gttccatcac gcgcagccgg gcgggctcga gagcgcgttg
       61 ctgcggcgcg cggccggcca gtgggcagcc ttcgcgatcg tgcaggcgat cgcgctcgtg
      121 cgctggaagg cgcagccggt ctggctgatg gtcgtcgcgg ggctgcgcgc gtccgacctg
      181 ctcaccgacc tgtcgtacat cgcggcggtg ccgtcgctga cgacgctggg atgggcgttg
      241 ctgacgccgc cggccttcct gaacgcggcc ttcatcatca cgatggtgct cgcgtatcgc
      301 cggctgacgc ccggcgcgcg atgagggccg ccgcacgggc ggggcgccga tgtgccggcg
      361 catccggcgg ggggtatgcg cggcgcgcgc tgtgcgctcg tgcaggccgg gctcctgccg
      421 cgtccctctg actccggatt tacccgtagt ccgagggccc gctgccctgc tgtcgaggcc
      481 cacttcattc gtcgcgccgg gcgaacagga ggacttcgtt gcgcttcagt ttggactgag
      541 tgcctcgggg cactgcatac tgtgaggatg agcgaagatt tcaagcccgg gacgagaccg
      601 cttttgcggc ttgccgggct cgatgccgta cgggcattcg tgtcgcgcgc cgacgaggcg
      661 cacgtcatct ggaccgccgt catggctttc atgttgcggc tttaccagga gcccgatggc
      721 aaggcccggc gcgagtccgg ccccacggac tcgcaggtgg tcacggcctt gcgcgcggtg
      781 ctgcaggaga acttcggcag cgagcccgcc gactacgtct cgtgggcgct ggtgcgctac
      841 ggctgccgcc agaagccgac gatgtcggcg ctgcatccct gggaaggcct gctgttcgag
      901 tggcagcagc gcaaggccag cacgccgcgc atcgcgttga tgctgcgcga cgccggcttc
      961 gagcggccgc tcacgccgca ggcgctcgag gcgctcgacg gctggatcac cgatcccgtc
     1021 acggcgctgg gcgcggccac cgacatcatc gaggcgctgt tcggcgagcg cctggtctcg
     1081 tacaacctgc acgacgacgg cagcacgccg tcgcacgacg tgctgttcgg cggcctcgtg
     1141 gcctcgctga tgccccaggc caagctcgcg ctgctgcgcc agagcctcgt gcggcaggac
     1201 gatggcgccg tctggcgcgt cgagtacacc tacgaaggcc gtgagcgcca tttcgatgtc
     1261 cgggccgacg gctcggccat ggacgtcgac ggcgtgatgc agggcctcga cgaactgatg
     1321 gagagcatcg gccgcgtcga gcgcgcgtac cgcctcgcgc cgggccgcta caacaacggc
     1381 gagacgggca ccttcgtcct cgtggacagc cagcgctttc ccgagatcgc gtggcgcctg
     1441 cgcctgccgc tgctgcgcag cccgagcgtg tccgaccttc cgccgggcgt gcccaccgcc
     1501 gcgccggagc cgctgcgcgt gggcgcgcgt ccgatggcgc cgcggccgca gccgcaggcg
     1561 gccagcaccg cgcgcgaggc cgtgcaggac gacgtcgcgg tcgcgacgcg accgggtggc
     1621 ggcgtgacgg cgccgatgtc gctgctcgag tcgcagttct cgccgcccca gcccgcggcc
     1681 gcgacgacgg cggctgccga gccgtcgggc ccgatcgagc gggccgccga tccggcgatg
     1741 aagtcgctga tcaacaacct gcgccgctcg ttcggcgcgg ggcgcctgct gcagggcggc
     1801 cgctggatga agcaggtccg caccgagccg cccgcctggc tcgccaatgg cagcgacccg
     1861 atccgcatct tctacgagaa gcaggagacg ctgttccgca agggccgcat cggctggggc
     1921 gcgctggtgg aggccgacga cggcgtgttc gcgcgcggcg aggacgacct gcccggcgtg
     1981 ctcgtctaca gcgaggacga gtacttcgac gcgcggcccg cggaacttgc ggcgatcggc
     2041 gcgcgcctgg tcgaactcaa ggccgcgggc gcggtcggcg cgttcgagcc cagccgcttc
     2101 gcgcagtcga tccgcaacga ctcgggccgg cccctgggcg tgacggtgcc cgcgtcgctg
     2161 agcgccaagg agccgatgct cagcagcttc atcgcgatcc gttcgcacct gcccgacggc
     2221 ctgctggccg gcgactggtt cccggtgctg atccacccgt ccagcgccgt gccgatgatc
     2281 gtgccctgca cgttctggcc cgacgcgatg gtcagcgcgt ggaacgagcg ccgcatgagc
     2341 ctgtagggcg ggttagccgc aaggcgtaac ccgccgcttg ccggcatgac gacccggccc
     2401 cgcgtttatg gaaaagagct tgcgtcccca ggcgggatcg cgaacggcgg gttacgcctg
     2461 cggc
//
LOCUS       DATCRO010000750         8032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_942433, whole genome shotgun sequence.
ACCESSION   DATCRO010000750 DATCRO010000000
VERSION     DATCRO010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942433"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..880)
                     /locus_tag="VIN75_20365"
     CDS             complement(167..880)
                     /locus_tag="VIN75_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY8976580.1"
                     /translation="MNPVQLDNNTHRDLRIVTTRGAAWGDDVMTTPVFPNEFRDVQAH
                     YPIVFQPADNGPGMQPVALLGLRNGENLFLEAHGWDASYIPLALERGPFMIGRGGSEL
                     TIHVDLDSPRIGHGEGEAVFLPHGAATDYLERMNSVLAAIHTGVEATAPFIATLLRHD
                     LIESFALDVQAADGSQNRLAGFSTINEERLARLDAAVVAELHAQGWLVAIFMVVASTT
                     HFRDLIARYNKRLVRQGLA"
     gene            complement(895..2445)
                     /locus_tag="VIN75_20370"
     CDS             complement(895..2445)
                     /locus_tag="VIN75_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8976581.1"
                     /translation="MRHVVILGGGSAGWLTAALLAREHHAGDPGGLAVTLIESPNTPP
                     IGVGEGTWPSMRDTLRRIGLSETTLVRECDAAFKQGSHFRGWMHGRAAAEGDQYRHPF
                     TLPMGFGDVDLVAGWQQRHGDRPFAEVVSAQPHVCMAGHAPKQAATPEFAAVANYGYH
                     FDAGKFGVQLRTHAIDKLHVRHVLDDVDQVLSHDDGDIAALRTRHHGDIAGDLFIDCS
                     GMASVLLGQHYGVGMRSQKHLLFNDTALALQVPYAAPDAPIACETIATAQPAGWTWDI
                     GLHDRRGVGHVYSSAHISHDEAERQLLAYVRATGGSGDLPTPRRITFDPGWRERFWHR
                     NCVAVGLSAGFVEPLEASALALVELSAAMISNEMPATRAEMALVEKRFNDAFTYRWER
                     IVDFLKLHYALSRREDSAYWREHRERATLPDRLAELLILWRHRAPYRQDFFRIEEVFP
                     AASYQYVAHGMGLLPEPSARMRHPSLGDAAEPYFRETAHLARRMLGALPLQRSLIDHI
                     RAHGLPRG"
     gene            complement(2625..5660)
                     /locus_tag="VIN75_20375"
     CDS             complement(2625..5660)
                     /locus_tag="VIN75_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976582.1"
                     /translation="MKIHHAPSFRRTRLASACGLLLAGVTAAHAQGAAPAAAAASAAD
                     KPDVQTVTVTGIRASLDTSMALKRDAHGIVDGITAEDVGKFPDTNLAEAMQRISGVSI
                     DRVAGEGSKVTVRGFGPDYNMILLNGRQMPATTVADTGPSGSRAFDFANLAAESISGL
                     EVWKTSSAAKPAGGIGATINIKTARPLDNYSGRIATVGVEGLWDQANKRLPVIDRGDQ
                     LTPEVSGIYSDTFADHTFGVALTGNYEVRNGGYNNVNVGNGWRNCLATQDNTNCWGGI
                     PQSGPGITNRPSGSGIYSVPQNIDYAVSGIHRERLNGQLTLQWQPAKSFTSTLDYTYS
                     QNHLDTKRNDISSWFNFGPSASSWTNGPAATPIVYTEFLNDPNHVDANGNPSPGPADV
                     AMGVAKFGVETTLRSLGLNLAWKPTDKLRLEMDAHSSNSTSGSDSPWGTNNVIGGASF
                     NRGNTTVDFSQDFPVMSIANAPINPALQEVTGSSFRNSYMKAKVEQLQLKGSWDATEQ
                     SSVDFGLNLTHVKNRTAYANVQQDSWGGATNPGDYPDNIWQVDTLSKYFSRMNGHDSP
                     ALYNNWLYGQFETVRDIAARVGNPAMYHASADFDGPGGTDRRTKENTLSLYTQYNADW
                     ELAHMPMNLSAGVRYEKTDVTAAAVVPTPTGSQWQAVNEFDLTYNGFAATTQKGSYKF
                     FLPSIDFDADLTKTLKARASYGDTIARPGWDAIQGGRSLDQLFRVDGGTGSQGNPSLK
                     PLLSHNLDFSLEWYYGKSSYASVGFFHKNVQNFIGQGQTTYSPPGLTTPVGGTYWNAA
                     TGAGGCATTDLQCIRDYIFAHFNGQPGVTQTGTANGHATGIIAGQSTDPALLFKVNQP
                     VNQHSASLRGWEFNVQNAFGNSGFGVSANYTLVMSGLKYNNANMNDQFALVGLSNSAN
                     VVGYFENDSVNARIAYNWRGQFLASTFDGSGPNPVYTEPYGQVDMTLGYNVDKHFSLQ
                     FDAINLNDGVVRQHSRTKEELESITQTGRRFMIGAHYKF"
     gene            6163..7041
                     /locus_tag="VIN75_20380"
     CDS             6163..7041
                     /locus_tag="VIN75_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976583.1"
                     /translation="MSLTVPSRAARLDPAWLDAEYDQRARHPEHVEIGARWETASSLV
                     QRLESWRRDVRYGPGEGETIDVYPTPAADAPVLVFIHGGYWRSSDKSLHAFLAPAFNA
                     DGALVMIPNYALCPSVGIEHIAQQLARCLAWVHRNAALYGGDPSRIVVAGHSAGGHLA
                     AMMLSCRWKDVDASLPPQLVSGALAISGLFDLEPLRLAPFIKDDIRLTPASVKRLSPA
                     FFPRPRKPLFTAVGAEETSELKRQTALIRDQWGPTSVPVSETIANANHFTILHNLADP
                     NGRLHRHALALLGIRR"
     gene            complement(7054..7320)
                     /locus_tag="VIN75_20385"
     CDS             complement(7054..7320)
                     /locus_tag="VIN75_20385"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HEY8976584.1"
                     /translation="MTQTLITLLIVLAAAGYSVWKLAPASLRHRLAAQTGTIARATGA
                     SEHTARRIEIKAMDAAANASSCGGGCGPCKGCATGQDKDDGASA"
     gene            complement(7325..>8032)
                     /locus_tag="VIN75_20390"
     CDS             complement(7325..>8032)
                     /locus_tag="VIN75_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEY8976585.1"
                     /translation="VLVFLPQILILFLFILYMAGIVSAMAVAWVLQRLGRRTGPAHHT
                     LMMELPAYRWPNPRNLAIGLWERARIFMTRVGTIILSLMIVLWFLSTFPGPPPGATGP
                     AIEYSLAGRLGHLLQYIFAPIGFNWQISIALVPGLAAREVAVGALGTVYALSASGDAV
                     AEQLSPLIHQTWSLPTACALLAWYVFAPQCVATLSVTRRETGGWRHPVIMASYLFALA
                     YVASGLTFYTVRALFGA"
BASE COUNT         1123 a         2604 c         2972 g         1333 t
ORIGIN      
        1 cccttgaggt aggccatcgc gtccgcggtg gagcggcgcg cggcctgcac catcggccag
       61 tgcgcgacga ggccgcgctg cacccagggc cgcgtgtcgc gcaggcgctt gccggacatc
      121 gcctcctcgg gcgtgccggc gagttcgcgg atgggtgcca tgtcgttcac gccaggccct
      181 ggcgcacgag ccgcttgttg taacgcgcga tcaggtcgcg gaaatgcgtg gtcgacgcca
      241 ccaccatgaa gatcgcgacc agccagccct gggcgtgcag ctcggcgacg acggcggcgt
      301 ccaggcgcgc gaggcgttcc tcgttgatcg tgctgaagcc ggccagccgg ttctgcgagc
      361 cgtcggcggc ctggacgtcg agcgcgaacg actcgatcag gtcgtgccgc aggagcgtcg
      421 cgatgaaggg cgccgtcgcc tcgacgcccg tgtggatcgc ggcgagcacg gagttcatgc
      481 gctccaggta gtccgtggcg gcgccgtgcg gcaggaacac ggcctcgccc tcgccgtggc
      541 cgatgcgcgg gctgtccagg tcgacatgga tcgtcagctc ggagccgcca cggccgatca
      601 tgaaggggcc gcgttcgagc gccagcggga tgtaggacgc gtcccagccg tgcgcttcca
      661 ggaacaggtt ctcgccgttg cgcaggccca gcagcgccac gggctgcatg cccgggccgt
      721 tgtcggccgg ctggaagacg atcgggtagt gcgcctgcac gtcgcggaat tcgttcggga
      781 agaccggcgt ggtcatcacg tcgtcgcccc aggccgcgcc gcgggtggtg acgatgcgca
      841 ggtcgcggtg ggtgttgttg tcgagctgga cgggattcat ggtggggctt ggtgtcagcc
      901 gcgcggcagg ccgtgcgcgc ggatgtggtc gatgagcgag cgctgcagcg gcagggcgcc
      961 gagcatgcgc cgcgccagat gggcggtctc gcggaaatag ggctcggcgg cgtcgccgag
     1021 gctcggatgg cgcatgcgcg cggacggctc gggcaggagg cccatgccgt gcgcgacgta
     1081 ctggtagctg gcggcgggga acacctcctc gatgcggaag aagtcctggc ggtacggcgc
     1141 gcggtggcgc cacaggatca gcagctcggc caggcgatcg ggcagcgtgg cacgttcgcg
     1201 gtgttcgcgc cagtaggcgc tgtcctcgcg ccggctcagc gcgtagtgca gcttgaggaa
     1261 gtcgacgatg cgttcccagc ggtacgtgaa cgcgtcgttg aagcgcttct cgaccagcgc
     1321 catctcggcg cgcgtggcgg gcatctcgtt gctgatcatc gcggccgaca gctccaccag
     1381 cgcaagcgcc gacgcctcca gcggttcgac gaagcccgcc gacaggccca ccgcgacgca
     1441 gttgcgatgc cagaagcgct cgcgccagcc ggggtcgaac gtgatgcggc gcggcgtggg
     1501 cagatcgccc gatccgccgg tggcgcggac gtaagcgagc agctgccgct cggcctcgtc
     1561 gtgcgagatg tgcgcgctgg agtagacgtg gccgacgccg cgccggtcgt gcaggccgat
     1621 gtcccacgtc cagccggcgg gctgggcggt ggcgatggtc tcgcaggcga tcggcgcgtc
     1681 gggcgcggcg tagggcacct gcagcgcgag cgccgtgtcg ttgaacagca ggtgcttctg
     1741 cgagcgcatg cccacgccgt agtgctggcc cagcaggaca gaggccatgc ccgagcagtc
     1801 gatgaacagg tcgccggcga tgtcgccgtg gtggcgcgtg cgcagtgccg cgatgtcgcc
     1861 gtcgtcgtgc gacagcacct ggtcgacgtc gtcgagcacg tggcgcacgt ggagcttgtc
     1921 gatcgcgtgc gtgcgcagct ggacgccgaa cttgccggcg tcgaagtggt agccgtagtt
     1981 ggccacggcc gcgaattcgg gggtggccgc ctgcttgggc gcatggccgg ccatgcacac
     2041 gtggggctgc gcgctcacga cctcggcgaa cggccggtcg ccgtggcgct gctgccagcc
     2101 ggcgaccagg tcgacgtcgc cgaagcccat cggcagcgtg aacgggtggc ggtactggtc
     2161 gccctcggcg gccgcgcgcc cgtgcatcca gccgcggaaa tgcgagccct gcttgaacgc
     2221 ggcgtcgcac tcgcgcacga gcgtcgtctc gctcaagccg atgcggcgca gggtatcgcg
     2281 catcgaaggc caggtgcctt cgccgacgcc gatcggcggc gtgttggggg actcgatcag
     2341 cgtgaccgcg agcccgcccg gatcgcccgc gtggtgctcg cgggcgagga gggccgcggt
     2401 cagccatccg gccgagccgc cgcccaggat cacgacgtgt ctcacgtcat cgttcgcgcg
     2461 catcatcatc tggcggtcat catgccggtc tgcaaaaggc gacgccaccc ggcgattccc
     2521 aggggaggga tcggcggatg gcgccgtggc gggcagcgga gctgcccgcc atcgccttca
     2581 ggcgtctggt gcggcgaagt tctcgccgcg tcaggcgatc acgctcagaa cttgtagtgc
     2641 gcgccgatca tgaagcggcg gccggtctgg gtgatcgatt cgagctcctc cttcgtgcgg
     2701 ctgtgctggc gcacgacacc gtcgttgagg ttgatcgcgt cgaactgcag cgagaagtgc
     2761 ttgtcgacgt tgtagcccag cgtcatgtcc acctggccgt agggctcggt gtacacgggg
     2821 ttcgggccgg agccgtcgaa cgtgctcgcc aggaactggc cgcgccagtt gtaggcgatg
     2881 cgcgcgttga cgctgtcgtt ctcgaagtag ccgacgacgt tggccgagtt gctcaggccc
     2941 accagcgcga actggtcgtt catgttcgcg ttgttgtact tcaggcccga catcaccagc
     3001 gtgtagttcg cgctcacgcc gaagcccgag ttgccgaacg cgttctggac gttgaactcc
     3061 catccgcgca ggctggccga gtgctggttg accggctggt tgaccttgaa cagcagcgcc
     3121 gggtcggtcg actggccggc gatgatgccg gtcgcgtggc cgttggcggt gccggtctgg
     3181 gtgacgccag gctggccgtt gaagtgcgcg aagatgtagt cccggatgca ctgcaggtcg
     3241 gtggtcgcgc agccgccggc gccggttgcc gcgttccagt acgtgccgcc cacgggcgtc
     3301 gtcaggcccg gcgggctgta cgtcgtctgg ccctggccga tgaagttctg cacgttcttg
     3361 tggaagaagc cgaccgaggc gtagctcgac ttgccgtagt accactcgag cgagaagtcc
     3421 aggttgtgcg acagcagcgg cttgagcgac gggttgccct gcgagccggt gccgccgtcg
     3481 acgcggaaca gctggtcgag cgagcggccg ccctggatcg cgtcccagcc cggacgcgcg
     3541 atcgtgtcgc cgtagctggc gcgcgccttg agggtcttgg tcaggtcggc atcgaagtcg
     3601 atgctgggca ggaagaactt gtagctgccc ttctgcgtgg tcgcggcgaa gccgttgtag
     3661 gtcaggtcga actcgttgac cgcctgccat tgcgagccgg tgggggtcgg gacgacggcg
     3721 gctgccgtga cgtccgtctt ctcgtagcgc acgcccgcgg acaggttcat cggcatgtgc
     3781 gcgagttccc agtcggcgtt gtactgggtg tacaggctca gcgtgttttc cttggtgcgg
     3841 cggtcggtgc cgccggggcc gtcgaagtcc gccgaggcgt ggtacatcgc cggattgccg
     3901 acccgggcgg caatgtcacg cacggtctcg aactggccgt agagccagtt gttgtacagc
     3961 gccgggctgt cgtggccgtt catgcggctg aagtacttgc tgagcgtgtc gacctgccag
     4021 atgttgtccg gatagtcgcc cgggttcgtc gcgccgcccc agctgtcctg ctggacgttg
     4081 gcgtaggcgg tgcggttctt cacgtgcgtc aggttcaggc cgaagtccac cgacgactgc
     4141 tcggtcgcgt cccacgagcc cttgagttgc aactgctcga ccttggcctt catgtagctg
     4201 ttgcggaacg acgagccggt gacttcctgc agcgccgggt tgatcggcgc gttggcgatg
     4261 ctcatcacag ggaagtcctg cgagaagtcg accgtggtgt tgccgcggtt gaacgaagcg
     4321 cccccgatca cgttgttcgt gccccagggg ctgtcggagc ccgaggtgga gttcgacgag
     4381 tgggcgtcca tctccaggcg cagcttgtcc gtcggcttcc aggccaggtt caggcccagc
     4441 gagcgcaggg tcgtctcgac gccgaacttg gccacgccca tcgcgacgtc ggccgggccg
     4501 gggcttgggt tgccgttggc gtccacgtgg ttggggtcgt tcaggaactc ggtgtagacg
     4561 atcggcgtgg cggcggggcc gttggtccag ctgctggcgg acgggccgaa gttgaaccac
     4621 gacgagatgt cgttgcgctt ggtgtcgagg tggttctgcg agtacgtgta gtccagcgtg
     4681 ctggtgaacg acttggccgg ctgccattgc agggtcagct ggccgttcag gcgctcgcgg
     4741 tggatgcccg agaccgcgta gtcgatgttc tgcggcaccg agtagatgcc cgagcccgac
     4801 ggacggttgg tgatgcccgg gcccgactgc gggatgccgc cccagcagtt cgtgttgtcc
     4861 tgggtggcca ggcagttgcg ccagccgttg cccacgttga cgttgttgta gccgccgttg
     4921 cgcacctcgt agttgccggt cagcgcgacg ccgaaggtgt ggtcggcgaa ggtgtcgctg
     4981 tagatgccgg agacctcggg ggtcagctgg tcgccgcggt cgatgacggg caggcgcttg
     5041 ttggcctggt cccacaggcc ctcgacgccg acggtggcga tgcggccgct gtagttgtcc
     5101 agcgggcgcg ccgtcttgat gttgatggtg gcgccgatgc cgccggcggg cttggcggcg
     5161 ctgctggtct tccacacttc caggccggag atggactcgg cggccaggtt ggcgaagtcg
     5221 aacgcgcgcg agccggacgg gccggtgtcg gccaccgtcg tcgccggcat ctggcggccg
     5281 ttcaacagga tcatgttgta gtcggggccg aagccgcgca ccgtcacctt cgagccttcg
     5341 ccggccacgc ggtcgatcga cacgccgctg atgcgctgca tggcttccgc gaggttggtg
     5401 tcggggaact tgccgacgtc ctccgcggtg atgccgtcga cgatgccgtg cgcgtcgcgc
     5461 ttgagggcca tcgacgtgtc caggctggcc cggatgcccg tgacggtcac ggtctggacg
     5521 tccggcttgt cggcggccga ggcggccgcc gcggcgggtg ccgcgccctg ggcgtgggcc
     5581 gccgtgaccc cggccagcag caggccgcag gccgaagcga ggcgggtacg ccggaacgac
     5641 ggcgcgtggt ggatcttcat tgacttgtct ccgatgtttt ggtgagccgt gtcgccgttc
     5701 gggaacgtgc gccccatgcc tgcccgttgt tgaccgcggg tttgaaagcg ctttcagagt
     5761 tgtttgcgtc gttccctgaa agcggtttca aatgtaagcc agctgtccag ccagtcagct
     5821 agggaacacc cgcatatcgt tgcgtttcga cttctccggg caacacttct tcaggattcc
     5881 gcgggtttgt cccgaggtga acagccgttc cggcgcgatg gctcggcccg gcccggcgga
     5941 ttggcggcgg ccggtggcga agcaagcgct gccgggtcga acggagccac ggggaggtgt
     6001 cgcgacggat tttcaggccg ccgggagcag ttgacagtcc ctaaagcaga aagcgctttc
     6061 atagcgtact cgacatgaca gcgatggacg cccgcgccgc ccgggccgat tgcactagga
     6121 tgggcgcgcc cgccaaccga ccattccagc aggagtcccg ccgtgagcct caccgtccct
     6181 tcccgtgccg cgcgcctgga tccggcctgg ctcgacgccg aatacgacca gcgcgcccgc
     6241 catccggagc acgtcgagat cggcgcccgc tgggagacgg cgtcctcgct cgtgcagcgc
     6301 ctcgagtcgt ggcggcgcga cgtgcgctac gggccgggcg agggcgagac gatcgacgtc
     6361 tacccgacgc ccgccgccga cgcgccggtg ctggtgttca tccacggcgg ctactggcgc
     6421 tcgtctgaca agtccctgca cgcgttcctc gcgcccgcgt tcaacgccga cggcgcgctc
     6481 gtgatgatcc ccaactacgc gctgtgcccg tcggtgggca tcgagcacat cgcgcagcag
     6541 ctggcgcgct gcctggcctg ggtgcatcgc aacgcggcgc tgtacggcgg cgatccgtcg
     6601 cgcatcgtcg tggccgggca ttcggccggc ggccatctgg cggcgatgat gctcagctgc
     6661 cgctggaagg acgtcgatgc gtcgctgccg ccgcagctgg tgagcggcgc gctggccatc
     6721 tcgggcctgt tcgacctgga gccgctgcgc ctggcgccgt tcatcaagga cgacatccgc
     6781 ctgacgccgg cgagcgtcaa gcgcctgagc ccggcgttct tcccgcgccc gcgcaagccg
     6841 ctcttcaccg cggtcggcgc ggaggagacg tccgagctga agcgccagac ggcgctgatc
     6901 cgcgaccagt gggggccgac gagcgtgccg gtcagcgaga ccatcgccaa cgccaaccac
     6961 ttcaccatcc tgcacaacct ggccgatccc aacggccggc tgcaccggca cgcgctggca
     7021 ttgctgggca tccggcgctg aggccggcgg cgctcaggcc gaggcgccgt cgtccttgtc
     7081 ctgcccggtg gcgcagccct tgcacgggcc gcagccgccg ccgcagctgc tcgcgttcgc
     7141 ggcggcgtcc atcgccttga tctcgatgcg gcgcgcggtg tgctcggacg cgcccgtggc
     7201 gcgcgcgatc gtgcccgtct gcgcggccag cctgtggcgc agcgacgccg gggcgagctt
     7261 ccacacgctg tagccggcgg cggccaggac gatgagcagg gtgatgagcg tctgggtcat
     7321 gaaatcaggc tccgaacagg gcgcgcaccg tgtagaaggt gaggcccgag gcgacgtacg
     7381 ccagggcgaa caggtagctc gccatgatga cggggtggcg ccagccgccc gtctcccggc
     7441 gcgtgaccga cagcgtggcc acgcactgcg gcgcgaacac gtaccaggcc agcagcgcgc
     7501 aggcggtggg cagcgaccac gtctggtgga tcagtggcga cagctgctcg gccaccgcgt
     7561 cgcccgaggc cgacagcgcg tagacggtgc cgagcgcgcc gacggccact tcgcgcgccg
     7621 ccaggccggg caccagcgcg atcgagatct gccagttgaa gccgatgggc gcgaagatgt
     7681 actggagcag gtggccgagg cggcccgcca ggctgtattc gatcgcgggg cccgtcgcgc
     7741 ccggcggcgg gccggggaag gtcgacagga accacagcac gatcatcagc gacaggatga
     7801 tggtgcccac ccgcgtcatg aagatgcgcg cgcgctccca caggccgatc gccaggttgc
     7861 gcgggttggg ccagcgatag gcgggcagct ccatcattag cgtgtggtgc gcggggccgg
     7921 tgcggcgccc caggcgctgc agcacccacg cgaccgccat cgcgctgacg atgccggcca
     7981 tgtacaggat gaacaggaac aggatcagga tctgcggcag gaacacgagc ac
//
LOCUS       DATCRO010000751         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_942446, whole genome shotgun sequence.
ACCESSION   DATCRO010000751 DATCRO010000000
VERSION     DATCRO010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942446"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VIN75_20395"
     CDS             complement(<1..660)
                     /locus_tag="VIN75_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976586.1"
                     /translation="MELCPLTKVQHRIRIGSPLPFSIRDASSQLLLAKDQVVSDPHEL
                     EALVERGACVDVDELRASGEPLAPEQLIRQWRQLAGTLADVLARMPAADAPAALDEAV
                     RELAALAEAAPDLAIFLLVRPDPADEAPYGVVRSLHAAAAAHLIALRLDWPRERCLTL
                     VKAAFTMNVGMLELQARMANLARLPNPQQRHAVQMHPAASLAALKASGVTDPEWLQAV
                     EQ"
     gene            850..1944
                     /gene="apbC"
                     /locus_tag="VIN75_20400"
     CDS             850..1944
                     /gene="apbC"
                     /locus_tag="VIN75_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY8976587.1"
                     /translation="MTISEAALTTALATVLDPHTGQPYVAARQLKNLRVGADGAVAFD
                     IELGYPARSQFGVVREALAAAAKSVDGVTAVTVGVASKVTAHQVQRGVALLPGVSNII
                     AVSSGKGGVGKSTTAVNLALALAAEGARVGLLDADIYGPSLPLMMGLDGRPESEDGKT
                     MKPLENHGVKVMSIGFLVDAEQAVVWRGPMATQALDQLLRQTSWGELDYLLIDMPPGT
                     GDIALTLSQRVPLTGAVIVTTPQDIALLDARKGVNMFEKVGVPILGIVENMAVHVCTN
                     CGHVEHIFGAEGGRKLAAQYKVPYLGALPLTMAIREQTDSGTPTVAAEPDGEIAALYK
                     TVARQVAVEVAGKAKDFSAKFPTINVSKST"
     gene            complement(2103..3167)
                     /locus_tag="VIN75_20405"
     CDS             complement(2103..3167)
                     /locus_tag="VIN75_20405"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF006083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8976588.1"
                     /translation="MADLIITDVSPIGPVLIMGAGSIGCYLGGRLALAGHDVHFVGRP
                     RVLDELRVHGLTITEPDGRTATVSPDMLSLHEEVPAGLGISLVLLCVKSSATPAAATA
                     LARALPIGTLVMCMQNGLHNAEVARSVAARLRFLSAMVPYNVAEIGPGQFLRGSGGTL
                     ALEEDDVTTAWLPTLAEAGLPAATHKDIRAVQWGKLLLNLNNPVNALSGLPLREELLN
                     PDLRATTAALIEEAQGVLAAARQRTAKVTPVPTSWLPRLMRLPTPLFKVIAGRMLRID
                     PRARSSMADDLALGRRTEIGELCGEVVKLAKSVGRRAPRNEKMLGLIRYVELTALAVG
                     KRERGPTDPAELRRTIETAI"
BASE COUNT          455 a         1129 c         1197 g          441 t
ORIGIN      
        1 ctgttcgacg gcctgcagcc attcggggtc ggtgacgccg ctggccttga gcgcggccag
       61 gctcgccgcc ggatgcatct gcaccgcgtg gcgctgctgc ggattgggca ggcgcgccag
      121 gttggccatg cgcgcctgca gctccagcat gcccacgttc atcgtgaagg ccgccttgac
      181 cagcgtcagg cagcgttcgc gcggccagtc cagccgcagc gcgatcaggt gggcggcggc
      241 cgccgcgtgc aggctgcgca cgacgccgta gggggcctcg tcggccggat cggggcggac
      301 gagcaggaag atggcgaggt cgggtgcggc ttcggccagc gcggccagct cgcgcacggc
      361 ctcgtcgagc gcggccgggg cgtcggccgc gggcatgcgc gcgagcacgt ccgcgagcgt
      421 gcccgcgagc tggcgccact gccggatcag ctgctcgggc gcgagcggct cgccgctcgc
      481 gcgcagctcg tcgacgtcga cgcaggcgcc gcgttcgacc agcgcctcga gctcgtgcgg
      541 atcgctgacg acctggtcct tggccagcag caactggctg ctggcgtcac ggatgctgaa
      601 cggcagcggg ctgccgatgc ggatgcggtg ctgcaccttc gtgagcgggc agagttccat
      661 gaggtgctcc ggtgggcctg ggacgccagc acggccgggc agtccccgtg cccggtgttg
      721 ccaaagggcg cgggataaac ttcgcggtgc gtgcctcccc cggcactgcc ttcgattctg
      781 cgcccggccg gcgaaagctg cagcccgttc gcacccgcag cccacgtcgc gccgcccggc
      841 gccgccccca tgaccatctc cgaagccgcg ctgaccacgg cgctcgccac cgtcctcgac
      901 ccgcataccg gccagccgta cgtcgccgcg cgccagctca agaacctgcg cgtgggcgcg
      961 gacggcgcgg tggccttcga catcgagctg ggctatccgg cccgcagcca gttcggcgtc
     1021 gtgcgtgagg cgctggccgc ggcggcgaag tcggtcgacg gcgtgacggc ggtcacggtc
     1081 ggcgtggcca gcaaggtcac cgcgcaccag gtgcagcgcg gcgtggcgct gctgcccggc
     1141 gtctccaaca tcatcgcggt ctcgtccggc aagggcggcg tcggcaagag cacgacggcc
     1201 gtcaacctcg cgctcgcgct cgcggccgag ggtgcgcgcg tgggcctgct cgacgccgac
     1261 atctacggcc ccagcctgcc gctgatgatg gggctggacg ggcgccccga atccgaggac
     1321 ggcaagacga tgaagccgct ggagaaccac ggcgtcaagg tgatgtcgat cggcttcctg
     1381 gtcgacgcgg agcaggcggt ggtctggcgc gggccgatgg ccacgcaggc gctcgaccag
     1441 ctgctgcgcc agacgagctg gggcgagctc gactacctgc tgatcgacat gcccccgggc
     1501 accggcgaca tcgcgctgac gctgtcgcag cgcgtgccgc tcaccggcgc cgtgatcgtc
     1561 acgacgccgc aggacatcgc actgctggac gcgcgcaagg gcgtcaacat gttcgagaag
     1621 gtgggcgtgc ccatcctggg gatcgtcgag aacatggccg tgcacgtctg cacgaactgc
     1681 ggccacgtgg agcacatctt cggcgccgag ggcggccgca agctggcggc gcagtacaag
     1741 gtgccctacc tgggcgcgct gccgctgacg atggccatcc gcgagcagac cgactcgggc
     1801 acgcccaccg tggcggccga gcccgacggc gagatcgccg ccctgtacaa gacggtggcg
     1861 cgccaggtgg cggtggaggt ggccggcaag gccaaggact tcagcgcgaa gttcccgacg
     1921 atcaacgtct cgaaatccac ctaggtccat ccagcgctgc catccgcagg gagcggagcg
     1981 cggtcgcagc atgcagcgat gggaaggcgc ctcgccagat ccagggtgcc aggtcttgcc
     2041 gggcaactcg tcaagcgtgg cgttgccgga caagacctgg caccgccaag ggggggagcc
     2101 cttcagatcg ccgtctcgat cgtcctgcgc agctccgccg ggtcggtcgg gccgcgttcg
     2161 cgcttgccca ccgccagcgc cgtcagctcg acgtagcgga tcagccccag catcttctcg
     2221 ttgcgcggcg cgcggcggcc gaccgacttg gccagcttga cgacctcgcc gcagagctcg
     2281 ccgatctcgg tgcggcggcc gagggccagg tcgtcggcca tgctggagcg cgcgcgcgga
     2341 tcgatccgga gcatgcgccc ggcgatgacc ttgaacagcg gcgtgggcag gcgcatcagg
     2401 cgcggcagcc agctggtggg caccggcgtg accttggccg tgcgctggcg cgcggcggcc
     2461 agcacgccct gggcctcctc gatgagcgcg gcggtggtgg cgcgaagatc gggattcagc
     2521 agttcctcgc gcagcggcag ccccgacagc gcattgaccg ggttgttcag gttcagcagc
     2581 agcttgcccc actgcacggc gcggatatcc ttgtgcgtgg cggccggcag gccggcctcg
     2641 gccagcgtgg gcagccaggc ggtggtgaca tcatcctcct cgagggccag cgtgccgccg
     2701 ctgccgcgca ggaactggcc cgggccgatc tcggccacgt tgtacggcac catcgccgac
     2761 aggaagcgca gccgcgccgc cacggagcgc gcgacctcgg cgttgtgcag cccgttctgc
     2821 atgcacatca cgagcgtgcc gatcggcagc gcgcgggcga gggcggtggc ggcggccggc
     2881 gtggcgctgc tcttgacgca tagcagcacc agcgagatcc cgagtccggc cggcacctcc
     2941 tcgtgcaggc tcagcatgtc gggcgagacg gtggccgtcc ggccgtccgg ctcggtgatg
     3001 gtcaggccgt gcacgcgcag ttcgtccagg acgcgcgggc gcccgacgaa gtgcacgtcg
     3061 tgcccggcga gcgccaggcg gccgccgaga tagcagccga tcgagcccgc gcccatgatg
     3121 agcaccggac cgatcggcga gacgtcggtg atgatgaggt cagccacgtc gctcgggcgt
     3181 ggcggtcagc cccggtcggt ttcgatcagg caacgggatg cg
//
LOCUS       DATCRO010000752         6217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1065919, whole genome shotgun sequence.
ACCESSION   DATCRO010000752 DATCRO010000000
VERSION     DATCRO010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065919"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /gene="paaZ"
                     /locus_tag="VIN75_20410"
     CDS             complement(<1..2045)
                     /gene="paaZ"
                     /locus_tag="VIN75_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY8976589.1"
                     /translation="MSATPVLQSLIAGRWIGSSAARDLRSAINGRLVAQTHAEEIDFG
                     ESLDFARRQALPSLLALDFQQRAAILKALATYLQDRKEELYAISAHTGATRPDSWIDI
                     EGGFGTLFAYASMGRRELPSSNIVHEGPAQQLGKVGQFMGTHVLVPKRGVAVHINAFN
                     FPMWGMLEKFAPSFLAGMPCIVKPATATSYVAEACVRLIEQSGLLPAGALQLVIGGSG
                     DLLDRLEETDVVTFTGSADTAAQLRVNANLIRRSVPFNAEADSLNCAILAPDVQPGDP
                     EFDLFVKEVMREMSAKAGQKCTAIRRAIVPAAQVDAVAAALRERLAKIRVGDPSIEGV
                     RMGALASRAQQADVAERLAQLAQGNEIVYGERDGFAPVGEGVADGAFFAPTLLLCRDA
                     MRNEAVHSIEAFGPVSTLMTYGDFDEALALAAKGRGSLVGSVVTKTPALAAQAVYHAA
                     AFHGRLLVLDRDAAGESTGHGSPLPMLKHGGPGRAGGGEELGGIRAVKHYLQRCAVQA
                     SPTMLTAITGEYVRGGKVTEDAIHPFRKHFEEIQVGDSLLTHRRTVSEADIVNFGGIS
                     GDYFYMHFDAVAAKDTQFGQRIAHGYFVLSAAAGLFVSPAPGPVLANYGLDTLRFVNP
                     VAIGDTIQARLTCKRRIDRGNRPDQPPQGVVAWDVQVTNQRGELVASYDILTLVAKKA
                     "
     gene            complement(2198..3355)
                     /locus_tag="VIN75_20415"
     CDS             complement(2198..3355)
                     /locus_tag="VIN75_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976590.1"
                     /translation="MKRIEKTLLALAGAALMGLGGSAWADINVGVTVSATGPAASLGI
                     PEKNTFSLMPKTIAGQKFNYIVLDDASDTTTAVANTRKLITEHKVDVVVGSTVTPNSL
                     AMIDAVSEAQVPMISMAASARIVEPMDAKKKWVFKTPQNDIMMALAIATHMQAQGVKT
                     VAYIGFADAYGEGWYGEFNKVAASKGLSIVANERYNRTDTSVTGQALKIMAARPDAVL
                     VGGSGTPAALPQKTLKERGYQGKYYQTHGVANNDFLRVGGKDVEGTFLPAGPVLVVDQ
                     LPADHPAKKSAKAYVTTYEGAYGKGSVSTFGGHAWDAGLLLAAAIPEAIKKAQPGTPQ
                     FRAALRDALEGVKEMPGAHGVFTMSPNDHLGLDQRARVMVKIENNTWKYQP"
     gene            complement(3488..4120)
                     /locus_tag="VIN75_20420"
     CDS             complement(3488..4120)
                     /locus_tag="VIN75_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8976591.1"
                     /translation="MARGRAPGFDATREEILAQAARLFANQGYPATSMNQVAEACEVS
                     KPTLYHYVRDKHELLAQICQNHLQHLVDLVDEVSAQGLPPQEHLRGLIHRFVQAYGEA
                     QNEHRVLTEDLKFLDDEHRERMVDVERQVVGRFADAVAAVRPELRGAHLHKPLTMLLF
                     GMINWTFTWLRPDGALTYEAVAPMVADLFFGGLGAVRTEPVEPRPAAQAS"
     gene            complement(4142..5221)
                     /gene="paaE"
                     /locus_tag="VIN75_20425"
     CDS             complement(4142..5221)
                     /gene="paaE"
                     /locus_tag="VIN75_20425"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY8976592.1"
                     /translation="MSLHFHPLRLRAKHQDTDDAVVLCFDVPEALRETFRFTQGQYLT
                     LRGDVDGQDLRRSYSICAGVDDGELRVGVRQVDGGAFSTWVHRHLKAGDEVQVFPPQG
                     RFHVPVDPSLARHYLGIAAGSGITPILSIMKTVLAREPMSRFTLIYGNRRPASTMFKE
                     ELEDLKNRYMTRLTLHHVFSREHVDAPLMAGRLDQAKLGDFLRGPVPAAGIDQVFVCG
                     PFEMNEQAEAALRAAGVPEDRIHIERFGTPEMQTGRPAPHEIHAEDAEAARVVVIRDG
                     VSREIEFRKTDPSLLDAAARAGMDVPFSCKSGVCSTCRCKLIEGEVRMDKNFALEKHE
                     VAAGFILSCQAHALTPRVVISFDER"
     gene            complement(5257..5853)
                     /gene="paaD"
                     /locus_tag="VIN75_20430"
     CDS             complement(5257..5853)
                     /gene="paaD"
                     /locus_tag="VIN75_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY8976593.1"
                     /translation="MNAALTPSHLDWLSSLTPVGPRAASSEATAGPTDLGCGRLSAAW
                     EVLSQVLDPEVPAVSLVELGIVRDLVETADGLEVVLTPTYSGCPATEAIEQSVRDALA
                     RFGTVTVTMRRAPAWTTDWITDAGREKLRQYGIAPPGPVDAAQGVPVRIVRRRETLAC
                     PRCGSTRTEQLSAFGSTACKSLYRCLACREPFEHFKPI"
     gene            complement(5913..>6217)
                     /locus_tag="VIN75_20435"
     CDS             complement(5913..>6217)
                     /locus_tag="VIN75_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY8976594.1"
                     /translation="AESTRRMQDALQQLWPYVNELFAADAVDAAAEASGLGPAWSSLK
                     PDWDAAMDEVLAEARLARPKDTPHVYAGVRGVHSEHMGHLLSTMQHLQRAYPGGVW"
BASE COUNT          919 a         2147 c         2162 g          989 t
ORIGIN      
        1 gccttcttgg cgaccagcgt gaggatgtcg tagctggcga cgagctcgcc gcgctggttg
       61 gtgacctgca cgtcccaggc cacgacgccc tgcggcggct ggtccggacg attgccccgg
      121 tcgatgcgac gcttgcaggt caggcgcgcc tggatggtgt cgccgatcgc caccgggttg
      181 acgaagcgca gcgtgtccag gccgtagttg gccagcaccg gccccggcgc cggggacacg
      241 aacagccccg ccgccgccga cagcacgaag tagccatgcg cgatgcgctg gccgaattgc
      301 gtgtccttgg ccgcgaccgc gtcgaagtgc atgtagaagt agtcgccgct gatgccgccg
      361 aagttgacga tgtccgcctc cgacaccgtg cggcgatggg tcagcagcga gtcgccgacc
      421 tggatctcct cgaagtgctt gcggaacgga tggatcgcgt cctcggtgac cttgccgccg
      481 cgcacgtact cgccggtgat cgccgtcagc atcgtcgggg aggcctgcac cgcgcagcgc
      541 tgcaggtaat gcttgaccgc gcggatgccg ccgagttctt cgccgccgcc ggcacgcccc
      601 gggccgccat gcttgagcat cggcagcggc gacccgtgac ccgtcgactc gccggccgcg
      661 tcgcggtcca gcaccagcag ccggccgtgg aaggccgccg cgtgatagac ggcctgcgcc
      721 gccagtgccg gcgtcttcgt caccaccgag ccgaccaggc tgccgcggcc cttcgccgcc
      781 agcgccagcg cttcatcgaa gtcgccgtag gtcatcagcg tcgacaccgg accgaaggcc
      841 tcgatgctgt gcaccgcctc gttgcgcatc gcgtcgcggc acagcagcag cgtcggcgcg
      901 aagaaggcgc cgtcagcgac gccctcgccc accggcgcga agccgtcgcg ctcgccgtag
      961 acgatctcgt tgccctgggc cagctgcgcc aggcgctcgg ccacatcggc ctgctgcgcc
     1021 ctggaggcca gcgcccccat gcgcacgccc tcgatcgacg gatcgccgac cctgatcttc
     1081 gccagccgct cgcgcagcgc cgcggccact gcgtcgacct gcgccgccgg cacgatcgcg
     1141 cgccggatgg cggtgcattt ctggccggcc ttggcgctca tctcccgcat cacttccttg
     1201 acgaagagat cgaactcggg atcgcccggc tgcacgtccg gcgccaggat cgcgcagttc
     1261 aggctgtccg cctcggcgtt gaacggcacc gagcggcgga tcaggttcgc gttgacgcgc
     1321 agctgcgccg ccgtgtccgc cgagccggtg aaggtgacga catcggtctc ctccagccgg
     1381 tccagcagat cgccggaacc cccgatgacc agctgcaacg cgcccgccgg cagcaggccg
     1441 gactgctcga tgagccgcac gcaggcctcg gcgacgtagc tggtcgcggt ggccggcttg
     1501 acgatgcagg gcatgccggc gaggaagctg ggcgcgaact tctccagcat cccccacatc
     1561 gggaaattga acgcgttgat gtggaccgcg acgccgcgct tgggcaccag cacgtgggtg
     1621 cccatgaact ggccgacctt gccaagttgc tgggccgggc cttcgtggac gatgttgctg
     1681 gacggcagtt cgcgacggcc catcgacgca taggcgaaga gcgtgccgaa gccgccttcg
     1741 atgtcgatcc acgagtcggg ccgggtggcg ccggtgtggg cggagatcgc gtagagctct
     1801 tccttgcggt cctgcaggta ggtggccagg gccttgagga tggcggcgcg ctgctggaag
     1861 tcgagcgcga gcagcgaggg cagcgcctgg cggcgtgcga agtcgaggct ttcgccgaag
     1921 tcgatttctt cggcatgggt ctgcgccacc agccggccgt tgatggcgct gcgcaggtcg
     1981 cgcgcggcgc tcgatccaat ccaccgaccg gcgatcaaac tctgaaggac gggtgtagcg
     2041 ctcatagcat tctcacaaca tcaaacgcaa ggacatttcg cggacgagcg gcgtagcgaa
     2101 cgggccgagg tcgggcctga ggcgcgcagc cgtactcccg tacggcgagc accgcaggcc
     2161 cgagatcggc ccgcgcagtc gccgagcgtc cgcgaaatca gggttggtat ttccaggtgt
     2221 tgttctcgat cttgaccatg acgcgggcgc gctggtcgag gccgaggtgg tcgttgggcg
     2281 acatcgtgaa cacgccgtgc gcgcccggca tttccttcac gccctcgagc gcgtcgcgca
     2341 gcgcggcgcg gaactgcggc gtgccgggct gggccttctt gatggcctcg gggatcgccg
     2401 cggccagcag cagtcccgcg tcccaggcgt gaccgccgaa ggtcgagacg ctgcccttgc
     2461 cgtacgcgcc ctcatacgtc gtcacatagg ccttggcgct cttcttggcc ggatggtcgg
     2521 ccggcagctg gtccaccacc agcaccgggc cggccggcag gaaggtgccc tcgacatcct
     2581 tgccgccgac gcgcaggaag tcgttgttgg ccacgccgtg cgtctggtag tacttgccct
     2641 ggtagccgcg ctccttgagc gtcttctgcg gcagcgccgc cggcgtgccg ctgccgccga
     2701 ccagcaccgc gtcgggcctg gccgccatga tcttcagcgc ctgacccgtc accgaggtgt
     2761 cggtgcggtt gtagcgttcg ttggcgacga tgctcaggcc cttgctggcg gcgaccttgt
     2821 tgaactcgcc ataccagccc tcgccgtagg cgtcggcgaa gccgatgtag gccaccgtct
     2881 tcacgccctg cgcctgcatg tgggtggcga tggccagcgc catcatgatg tcgttctgcg
     2941 gcgtcttgaa cacccacttc ttcttggcgt ccatcggctc gacgatgcgc gccgacgccg
     3001 ccatcgagat catcggcacc tgtgcctccg acaccgcgtc gatcatcgcc agcgagttcg
     3061 gcgtcaccgt cgagcccacc accacgtcga ccttgtgctc ggtgatcagc ttgcgggtgt
     3121 tggccaccgc ggtcgtcgtg tccgacgcat cgtccagcac gatgtagttg aacttctgcc
     3181 cggcgatggt cttgggcatc agcgagaagg tgttcttctc cggaatgccc agcgacgccg
     3241 ccggcccggt ggccgacacg gtcacgccga cgttgatgtc ggcccaggcg ctgccgccca
     3301 ggcccatcag cgcagcgccg gccagggcca gcagggtctt ctcgatgcgt ttcatgtcgt
     3361 ctccttcgga tgtgttttca ggaacgccgg gatcaatggg actgctactg caaggacttc
     3421 gaggacttcg aggactgcgg ggatttcggg caccgcgggc cagcggcgat gctccgccgc
     3481 gaaaccttca tgaggcttgc gccgcgggcc gcggctcgac cggctcggtg cgcaccgcgc
     3541 cgaggccacc gaagaacagg tcggccacca tcggcgccac cgcctcgtag gtcagcgcgc
     3601 cgtcgggccg cagccaggtg aaggtccagt tgatcatgcc gaacagcagc atggtcagcg
     3661 gcttgtgcag atgggcgccc cgcagttccg gccgcaccgc ggccaccgcg tcggcgaagc
     3721 ggccgacgac ctggcgctcg acatcgacca tgcgctcgcg gtgctcgtcg tccaggaact
     3781 tcaggtcctc ggtcagcacg cggtgctcgt tctgggcctc gccataggcc tgcacgaagc
     3841 gatggatcag gccgcgcagg tgctcctgcg gcggcaggcc ctgcgcgctc acctcgtcga
     3901 ccaggtcgac caggtgctgc aggtggttct ggcagatctg cgcgagcagc tcatgcttgt
     3961 cgcgcacgta gtggtagagc gtgggcttgc tgacctcgca ggcctcggcg acctggttca
     4021 tcgaggtggc cgggtagccc tggttggcga aaagtcgggc ggcctgcgcg aggatctcct
     4081 cgcgggtggc atcgaatccg ggggcgcgac ctcgggccat ggggggtgtt ttccttgtga
     4141 atcagcgttc gtcgaacgag atcacgacgc gcggcgtcag ggcgtgggcc tgacagctga
     4201 ggatgaatcc ggccgccacc tcgtgcttct cgagggcgaa attcttatcc atccggacct
     4261 cgccctcgat cagcttgcaa cggcaggtcg agcagacgcc ggatttgcag gagaacggca
     4321 cgtccatccc ggcccgcgcg gcggcatcga gcaggctggg atcggtcttg cggaactcga
     4381 tctcgcgcga gacgccgtcg cggatcacga ccaccctggc ggcctcggcg tcctccgcat
     4441 ggatctcatg cggcgccggg cggccggtct gcatctccgg cgtgccgaag cgctcgatgt
     4501 ggatgcggtc ctccggcacg cccgcggcgc gcagcgcagc ctcggcctgc tcgttcatct
     4561 cgaacgggcc gcagacgaag acctggtcga tgccggcggc cggcaccggg ccgcgcagga
     4621 agtcgcccag cttggcctgg tcgagccggc ccgccatcag cggtgcatcc acatgctcgc
     4681 gcgagaacac gtgatgcagc gtcagccgcg tcatgtagcg gttcttcagg tcctcgagct
     4741 cttccttgaa catcgtggac gccggccggc gattgccgta gatcagggtg aagcggctca
     4801 tcggctcgcg cgccagcacc gtcttcatga tcgacaggat gggcgtgatg cccgagcccg
     4861 ccgcgatgcc gaggtaatgc cgcgccagcg acggatccac cggcacgtgg aagcgccctt
     4921 gcggcggaaa gacctggacc tcgtcaccgg ccttcagatg ccgatgcacc caggtggaga
     4981 aggcgccgcc gtcgacctgg cgcacgccga cgcgcaactc gccgtcgtcc acgccggcgc
     5041 agatcgaata cgaccggcgc aggtcctggc cgtcgacgtc gccgcgcagc gtcaggtact
     5101 ggccctgcgt gaagcggaag gtctcgcgca gcgcctcggg cacgtcgaag cacagcacga
     5161 cggcatcgtc ggtgtcctgg tgcttggcgc gcaggcgcag cggatggaaa tgcaggctca
     5221 tggcgtgggc tcggagtctt gaaaggtcgc cgatgctcag atcggcttga agtgttcgaa
     5281 gggctcgcgg caggcgaggc agcggtacag cgacttgcag gccgtcgagc cgaaggccga
     5341 cagctgctcg gtccgcgtgc tgccacagcg cgggcaggcg agcgtctcgc ggcgtcgcac
     5401 gatgcggacc ggcacgccct gcgccgcgtc gaccggcccg ggcggcgcga tgccgtactg
     5461 gcgcagcttc tcgcggccgg cgtcggtgat ccagtcggtg gtccaggccg gcgcgcggcg
     5521 catcgtcacg gtgaccgtgc cgaaacgggc cagcgcatcg cgcacgctct gctcgatcgc
     5581 ctcggtcgcg gggcagccgg aataggtggg cgtcaacacc acctccagcc catcggccgt
     5641 ctcgaccagg tcgcgcacga tcccgagctc caccagcgac accgccggca cttcgggatc
     5701 gagcacctgc gacagcactt cccaagcggc ggacaggcgc ccgcagccga ggtcggtggg
     5761 gccggcagtg gcctcggacg aggccgcacg cgggccgacc ggcgtcagcg acgacagcca
     5821 gtccaggtgc gagggcgtca gggcggcatt catggatgtc ctcgaagcgt gggcgtccgg
     5881 gcgagggtca cgggatcatg cggtcgccgc gctcaccaca cgccgccggg ataggcgcgc
     5941 tgcaggtgct gcatcgtcga cagcagatgg cccatgtgct cgctgtggac gccgcgcacg
     6001 ccggcgtaga cgtgaggggt gtccttcggg cgggccaggc gcgcctcggc cagcacctcg
     6061 tccatcgcgg cgtcccagtc cggcttgagc gaagaccagg ccggccccag gccactggcc
     6121 tcggcggcgg catcgacggc gtccgccgcg aacagctcgt tgacataggg ccacagctgc
     6181 tggagcgcgt cctgcatgcg gcgggtcgat tccgcgc
//
LOCUS       DATCRO010000753         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_267615, whole genome shotgun sequence.
ACCESSION   DATCRO010000753 DATCRO010000000
VERSION     DATCRO010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267615"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VIN75_20440"
     CDS             complement(<1..1759)
                     /locus_tag="VIN75_20440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY8976595.1"
                     /translation="MNRFALAQKATPALALSALLLASLTACGGGSSSETLAQPTAASD
                     TPAQPASENALDDSAVASVLAQTEVLPAYHMAPALLDEPAQDDVGGTNASATVAPKTF
                     DVDADDAAIDTARLSRDVLAQRILEKQRARIASTGGDTNAAPLTTTIKGTVYTPAQIR
                     AAYGLSPLPAVGTTLTAAQKAALGAGQTIYLVDAYHDATALSDLNAFSTKFGLPTCTK
                     VSITTNAKLAAAPSNCTFAQVYSYTRGTVTTTAPAYNATWAPESKLDVQWAHAIAPLA
                     RIVLIEMPSAMSNAILDANTLARNLGPGAVSMSFGSAEAGWASTTDSYFVGTGMTFVA
                     AAGDSGAQVLWPAVSPNVLAVGGTSTNVSTTGSRVEIAWSGSGGGMSAYEALPAWQSG
                     VTPAGGNALVRRAVPDVAFNASPMTGEYVAVTLPGKATTWSAYGGTSIAAPQWAGLVA
                     VANAIRASNAKAALGDIHALIYKSIAAVPGTYAAAMHDVIEGTNGTCATCKAGSGFDQ
                     ATGWGSPNASQLFSVLTGVTSTQLATATAPTVPGGSLTGKVGTALSQSLNITAPTGTT
                     TSYALSGAPSGLSADSTGML"
BASE COUNT          416 a          801 c          953 g          438 t
ORIGIN      
        1 tgagcatgcc ggtgctgtcg gcggacaggc cgctcggcgc gcccgagagc gcatagctcg
       61 tggtcgtgcc cgtcggcgcc gtgatgttca gcgactggct cagggcggtg ccgaccttgc
      121 cggtgagcga gccgcccggg acggtcgggg ccgtggccgt ggccagctgc gtgctggtca
      181 cgccggtcag caccgagaac agctgcgacg cgttgggcga gccccagccg gtggcctggt
      241 cgaagcccga gccggccttg caggtggcgc acgtgccgtt ggtgccttcg atgacgtcgt
      301 gcatggccgc ggcgtaggtg ccgggcaccg ccgcgatcga cttgtagatc agcgcgtgga
      361 tgtcgcccag cgcggccttc gcattggacg cgcggatggc gttggccacc gcgacgaggc
      421 cggcccattg cggcgcggcg atgctggtgc cgccgtaggc gctccaggtc gtggccttgc
      481 cgggcagggt gacggcgacg tactcgccgg tcatcgggct ggcgttgaag gcgacgtcgg
      541 gcacggcgcg gcgcacgagg gcattgccgc cggccggcgt cacgccgctc tgccacgccg
      601 gcagggcctc gtaggcgctc atgccgccgc cgctgccgga ccaggcgatc tcgacacgcg
      661 agccggtggt cgacacgttg gtcgacgtgc cgcccacggc cagcacgttc ggcgagaccg
      721 ccggccacag cacctgcgcg ccggagtcgc cggcggcggc cacgaaggtc atgccggtgc
      781 ccacgaagta ggagtcggtc gtcgacgccc agccggcttc ggccgagccg aagctcatcg
      841 agacggcgcc cgggcccagg ttccgggcca gggtgttggc gtcgaggatg gcgttggaca
      901 tcgcgctggg catctcgatc agcacgatgc gcgcgagcgg ggcgatggcg tgcgcccact
      961 ggacgtcgag cttgctttcc ggcgcccacg tggcgttgta ggccggcgcc gtggtggtga
     1021 cggtgccgcg ggtgtaggag tagacctgcg cgaacgtgca gttcgacggc gcggccgcca
     1081 gcttggcgtt ggtggtgatc gagaccttcg tgcaggtcgg caggccgaac ttggtcgaga
     1141 acgcgttcag gtccgacagc gcggtggcgt cgtggtatgc gtcgacgagg tagatcgtct
     1201 ggcccgcgcc cagcgcggcc ttctgcgccg cggtcagcgt ggtgccgacg gccggcagcg
     1261 gcgacaggcc gtaggcggcg cggatctgcg ccggcgtgta gacggtgccc ttgatggtgg
     1321 tcgtcagcgg cgcggcgttg gtgtcgccgc ccgtggaggc gatccgggcg cgctgctttt
     1381 ccaggatgcg ctgggccagc acgtcgcgcg acaggcgcgc cgtgtcgatc gcggcatcgt
     1441 cagcgtcgac gtcgaaggtc ttcggcgcga cggtcgccga ggcgttcgtg ccgccgacgt
     1501 cgtcctgcgc gggctcgtcc aggagcgcgg gggccatgtg gtaggccggc aggacttccg
     1561 tctgggccag cacgctggcg acggcgctgt cgtcgagcgc gttctcgctg gccggctggg
     1621 caggggtgtc ggaggcggcg gtgggctgcg ccagggtttc ggagctggac cccccgccgc
     1681 aggccgtcag gctggcgagc aggagagcgg agagagccag ggcgggggtg gccttctgag
     1741 ccagggcaaa acgattcaag accgatcctt cgatgatgct gtgttcatcg gcggtcggcg
     1801 cttggacgag atgtgccagt gcctgcaaag gcatccggga cagcacgcca gcacgccggc
     1861 acgccgggtg tggacaagca gcaggaccaa tgaaggacca gccgccaggc gctgttgggt
     1921 cgacgcgggc gatcaggccg gcgtatcgat tcagagcagc gagtgcgtcg cgttcgagaa
     1981 aatattcgag cccgactgat gggttgccgc tttgcaacgg cgagcgaata ttaccgtagc
     2041 atctactcga caataaggga agatgcaaca agacgcagct gaacagtaac cctgaggttt
     2101 tgttacgttc gtcacgtcat ggttgagcgt tcagtcagcg gattcctcca cttttcgcgc
     2161 agttcacgca ccaccgtgaa aactcgattc gaatatcgtt gccgctcggc cacgattggt
     2221 ccgcggcatc tcgcaaaacc gcgggaaata tccggcgggc acgaaaattc aaatccctgg
     2281 gcgcatgcac gcaggtgaac tcagcgggag gttcgccgcg actgacccgc atgtccaggg
     2341 gcttggcacg cgatcggcgg gttgccggct tgccctccat aatcgattct gtatttatca
     2401 aggcaaatgg gaaagctgcc catattaatc tcttccataa cggtctccgt cgttgccatt
     2461 taatgctgct cgacaggagc ttgaaaccgc ttcacagacc ggaaaagaat cgctcacgga
     2521 atttgatgcc taaccaccgg atgcagcgaa aacgtcgcgt atggacgagt agaaatgcgt
     2581 caaacttgcc ctacacacct gggaaaga
//
LOCUS       DATCRO010000754        11390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_796362, whole genome shotgun sequence.
ACCESSION   DATCRO010000754 DATCRO010000000
VERSION     DATCRO010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796362"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..2645
                     /locus_tag="VIN75_20445"
     CDS             285..2645
                     /locus_tag="VIN75_20445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY8976596.1"
                     /translation="MSNRQDPFLDFESSPAPGGAGEGARLNLEAEGAVDAIPELDAVG
                     VDHDALLHSMVYLTRHHGKERSADSLLDGMPIEGLLNPDQAVRVMRAAGYNAGLMQRA
                     IGDIHALLLPAILLLANGDACVLVRRLDTPRGQSPVCEIVMPGQEFHSCKATEAELEH
                     EYLGFALVATPLPEQAPGARQEQALLDPNGHWLWGTLRRFIPYYRSAMVAALLSNVLM
                     LVTGLVTSVIYDKVIPHQGFVTLWALAAGALIALVFDLVSRQLRSHLIDTAGKKADLI
                     IGSLLFRQTLGVRMEHRPDSAGNYAHHLAQIEIVREFFAGATLSAVSDVPFIAMYIAM
                     TFVIGGPLGWVLVLAIPALVVMALAVQRALRRAMSANLRHQADLQGVIVEAIEGLEDL
                     KAVGAQGRFLHRFEDATSAAAEAGLMARWMMGWTNNISGVSQQLITLVMLVWGVYLIH
                     DGVVTGGALIGAVMYAGRAVMPVNSVVSLASRYQGARVAMLSIDKMMQQPTEREQGRA
                     YVTGHKVTGAIGLNDLSFSYPNTGDAGVAPPRVLKNATIRVNAGERIAILGRIGSGKS
                     TILRLMAGLYQPSEGYVDVDGLDLRQVDPADYRMRVGFVSQEPRLFKGTLRENVMMGR
                     PQLDAGKLAEVAKVTGLDRVVAGHPQGWELSVGEMGGLLSGGQRQLVALARCLITKPQ
                     ILLMDEPTSSMDAQSEVMFLRQLKEAAGTRTLIMVTHRPAVLELVDRVIVVDGGKIVL
                     DGPKGAVLAALSGIKPQAQPQVQNRPATEQPMQRQPAPAMERAQAA"
     gene            2656..3939
                     /locus_tag="VIN75_20450"
     CDS             2656..3939
                     /locus_tag="VIN75_20450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976597.1"
                     /translation="MAKFTLPFKLPFLKSPEPALAVPQMGGRARRGDAAFMSGVRAAQ
                     VVESAPHVTWVLYLMAAALASALTWAAVARVDEVTKADARVVPEGREQVIASLEGGIL
                     RELFVKEGEQVEEGQDLAELDPTRFKTQQAEGAAKILAMKAQIARLTAEAYNRPLVFP
                     PEVEAVPDVAQGERDSYLARKRANDDALGSNQRGMDMLRRELQMSEAMSAKGLLSEVE
                     VLHLRRQVNEMSLQSEDRINRFRQDASSELAKLRTDLAALQEQQAGRQDVLERTMIKS
                     PVRGIVKNIRINTLGGVIGGGAPIMEIVPIGRQSLIEARVKPGEIGFLQVGQTAKVKL
                     AAYDFAIYGAVDGKIESISPDALGDPERAVGSADPTYYRVMLRVDNNTLHEKGKTLPL
                     LPGMTGSVEVRIGERSVLNFLLRPMLKSKEAFRER"
     gene            3965..5071
                     /locus_tag="VIN75_20455"
     CDS             3965..5071
                     /locus_tag="VIN75_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976598.1"
                     /translation="MTGRITEIFGGLQQRHLINEQVAAYRDYTQAEVFFHLAREKGQR
                     VEQLRQRFAGRRIEKLFVGNNLVPAGFQLPGVETVALEKNHFERAMKGELQDMVARFA
                     GGIVLINNNDLAVGESAPGYVRVFAGSPDTIFLGWDYDNHHWMESSLALAAHTDLYFP
                     AHNENMYLLSKINALTSGPVPCACVQWSQSFLSDNLGEMLRRQRSDEPLGMHFFYQQF
                     AFRNQTVVTLNQKYKSIGFAERSFQAKTPFDRLAEWMDHKAHWIIPVLNDVPIRIFDA
                     LASGGIPIVPESLRFLAPISGLPRDMVVFYGPDDIFNPQAVVARALELFDEGGQGGIA
                     ARHRHALAHHHGADRLRRMVAMAQERLEFAPLIG"
     gene            5135..6148
                     /locus_tag="VIN75_20460"
     CDS             5135..6148
                     /locus_tag="VIN75_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY8976599.1"
                     /translation="MTQVLVTGGAGFLGSHLCDRLIARGDEVLCVDNFYTGGKGNVRH
                     LMGNKNFELMRHDVTFPLYVEVDRIFNLACPASPVHYQHDPVQTTKTSVHGAINMLGL
                     AKRTKARILQASTSEVYGDPTMHPQREEYWGNVNPIGPRSCYDEGKRCAETLFFDYWR
                     QHQLQIKIIRIFNTYGPRMQPDDGRVVSNFIVQALKGEPITLYGDGLQTRSFCYVDDL
                     IEGMMRLMDSDKAITGPINVGNPVEFTMRELAELVVQITGSKSPITFRPLPTDDPKQR
                     RPDITQAQDKLGWEPKVPLADGLKETIAYFRRTLDFTADLAPATVLPAMPPIPNIAAA
                     LAR"
     gene            6201..7649
                     /locus_tag="VIN75_20465"
     CDS             6201..7649
                     /locus_tag="VIN75_20465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8976600.1"
                     /translation="MGTPQNRSTWPAALTLAFALGAALLSPAAPAATARRATTAPASA
                     APATSSNSRCISDESTDPPTVPAFRTVPADSGSAAQAGAVAAPRMNLLAMVRSAIHRS
                     NAVGAARLLSEAAAIDIDEAHAAYWPQVGISGQVGGIDAGAPNSPSVHGKQFQGGINV
                     SAPLYDGGHTRDMTDYRTHLAEVAKFGEVSTQEQVALQTVSLALDRSRYTVEAQVYDQ
                     YARKMSCLVEALEMIVSADRGRTSELVQARKTQQQAELQRDQVLSQKRLTEIRLRRFI
                     GDELPPTEGITSVMLDTPALPDLLTMASRAAEIQSLDSQAAALDSYVKAVLDGQKPQL
                     GVLVAATKSAGAGDSRSVSGGITFNWQVFNAANKYTTEAARKRAAAARMNREDALEAR
                     KSRMAEVHEQAEHAMERAHSVIEILHNSELVRNFTLQQWQQLGRRSLFDVMAAEGDHY
                     NMRVQYVDALYDTQQSNALLWSLGLGLANHLE"
     gene            7838..9841
                     /locus_tag="VIN75_20470"
     CDS             7838..9841
                     /locus_tag="VIN75_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8976601.1"
                     /translation="MQPSSQLAPSAAAHRAPGSMPADPKSAALAAVAAPGAASIVIDR
                     NLLLQGIALQNEGKLEQAQAVFELYLTHIPNEGVALYSLAVVLMERFGAERALPVVER
                     GVQVASTFPPIWYAAGAVQQRLGLRDAAIRSYDKALELKPDYVEALINSGTLLREQLD
                     HHAAIDRFNRALALKPDHLNAMSNLAIMLTEFKRSEEAVAMFERLLAIKPDFEYGVGS
                     ICYERMHACDWSHFDSDVAKILEGIEAGKPVAKSLGVMSITDDAAVHHRCAEIFAAHR
                     YTPRHAPLWTGQRYRHDRIRIAYVSPDLREHPVGHLMAGIFERHDRRRFETIAISIGA
                     NDGSRLRQRMVACFDHFIDAKMMESRKIAELMRSMEVDIAIDLAGFTADSRSEVFGMR
                     PAPVQVNYLGYPGTMGTQYMDYVVADRHVIPPEHARHYNEKVVYLPDAYLPAASGLQV
                     AERTPTRAECGLPEEGVVFCSFNHDYKIAPHVFAVWMNLLREVPGSVLWLMSRSPLSQ
                     KNLRKEAAARGVDPDRLVFAQRVPRVEDHLARYRQADLFLDTHPYNAHTTCADALMVG
                     LPVVTCMGGAFPSRVAGSLLHAAGVPELVTSSLADYEALALQLARDPARLAAIKGKLA
                     ATRTSTALCDADGFTRNLEAIYTSMWRQSQLGGARDALSGGAQ"
     gene            9838..11115
                     /locus_tag="VIN75_20475"
     CDS             9838..11115
                     /locus_tag="VIN75_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976602.1"
                     /translation="MSAAVADAPAVSPAIAANDALARGNLQLADYLCREALRADANDA
                     LAMSLLGHMASGLRRFQLAESWFSRAVALRPASAEIAAWRDRAREMAQAARAHAASAA
                     PRALPRPKYLLVKAWGYGFWSDMDHVLGQLLLAELTGRTPVVHWGANTLFGAAGVANA
                     FELYFRPVSGATQDGLAAWGESFFPAKWSAANLRDENVNKFQGPGSRTSALHLLARDE
                     DVVVSDFHGKVVDLMGWIEPDSPWHGLDYQAVYRRLFAKYVQLQPALQARVDGFARER
                     MAGRRWVAVHVRGSDKITELRDLELVNQQYQHRLWTMLQADPAMGIFLLTDSERFARE
                     YREKYGDRVLAADVARSGDATGVHYAGHEGRKLGEEVVMDAFLAARCDAFLGNGASNV
                     STAIRHLKDWTPGSFELIGPDFLAMPNQMLHRW"
     gene            11184..>11390
                     /locus_tag="VIN75_20480"
     CDS             11184..>11390
                     /locus_tag="VIN75_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976603.1"
                     /translation="MTAPIVIDGVFFQIGRSGIARVWQSLLGLWAGTPFGDRLVVMDR
                     NRTMPRLPGIRYVDAAALNYNDLES"
BASE COUNT         1809 a         4133 c         3852 g         1596 t
ORIGIN      
        1 ggcgaaccgc caagaagggg ccggcgtcca agacctcggc ccgcggcaag ccggtcgcca
       61 agcggtccaa gcgctgagcc ggccgcgttc ttgagcagca aaggccccgc gttccggggc
      121 cttttcccat tcaaggcgca aggaataggg ccgggttgac ccgtctgatg tgctcaatgc
      181 ggcgctggac ggcatcgcag aatcgctgga accgaacttc catgaccgga aatcccgcga
      241 tgcccgcccg tcccgcctgc cggatgcccc aggactgacg tcccatgagc aaccgtcagg
      301 accccttcct ggacttcgaa tccagccccg cgcccggcgg ggcgggggag ggcgcgcgcc
      361 tgaacctcga ggccgaaggc gcagtcgacg cgatccccga gctggacgcc gtcggcgtcg
      421 accacgacgc gctgctgcac tcgatggtct acctgacccg ccaccacggc aaggagcgct
      481 cggccgactc gctgctggac ggcatgccca tcgagggcct gctcaacccc gaccaggcgg
      541 tgcgcgtcat gcgcgcggcg ggctacaacg ccggcctgat gcagcgcgcc atcggcgaca
      601 tccacgcgct gctgctgccc gccatcctgc tgctggccaa cggcgacgcc tgcgtgctgg
      661 tgcgccgcct ggacacgccc aggggccagt ccccggtgtg cgagatcgtc atgccgggcc
      721 aggaattcca cagctgcaag gccaccgagg ccgagctgga gcacgagtac ctgggcttcg
      781 cgctggtggc cacgccgctg cccgagcagg cgcccggcgc gcgccaggag caggcgctgc
      841 tcgacccgaa cggccactgg ctgtggggca cgctgcgccg gttcatcccg tactaccgct
      901 cggcgatggt cgccgcgctg ctcagcaacg tgctgatgct ggtcaccggt ctcgtgacct
      961 ccgtcatcta cgacaaggtc atcccgcacc agggtttcgt cacgctgtgg gcgctggccg
     1021 cgggcgcgct catcgcgctg gtgttcgacc tcgtgtcgcg ccagctgcgc agccacctga
     1081 tcgacacggc cggcaagaag gccgacctca tcatcggctc gctgctgttc cgccagacgc
     1141 tgggcgtgcg catggagcac cggccggact cggccggcaa ctacgcgcac cacctcgcgc
     1201 agatcgagat cgtgcgcgag ttcttcgccg gcgcgacgct gtcggccgtc agcgacgtgc
     1261 cgttcatcgc gatgtacatc gccatgacct tcgtgatcgg cggcccgctc ggctgggtgc
     1321 tggtgctggc gatcccggcg ctggtggtca tggcgctggc cgtgcagcgc gcgctgcgcc
     1381 gcgcgatgag cgccaacctg cgccaccagg ccgacctcca gggcgtcatc gtcgaggcca
     1441 tcgagggcct ggaggacctg aaggccgtcg gcgcccaggg ccgcttcctg caccgcttcg
     1501 aggacgccac ctccgccgcc gccgaggccg gcctgatggc gcgctggatg atgggctgga
     1561 ccaacaacat ctcgggcgtc tcgcagcagc tgatcacgct cgtgatgctg gtctggggcg
     1621 tctacctgat ccacgacggc gtggtgaccg gcggcgcgct gatcggcgcc gtgatgtacg
     1681 ccggtcgcgc ggtgatgccc gtcaacagcg tcgtgagcct ggcctcgcgc taccagggcg
     1741 cgcgcgtggc gatgctgtcg atcgacaaga tgatgcagca gccgaccgag cgcgagcagg
     1801 gccgcgccta cgtgacgggc cacaaggtga ccggcgcgat cggcctgaac gacctcagct
     1861 tctcgtaccc gaacacgggc gatgcgggcg tcgcgccgcc gcgcgtgctc aagaacgcga
     1921 cgattcgcgt caacgcgggc gagcgcatcg ccatcctggg ccgcatcggc agcggcaagt
     1981 cgaccatcct gcggctgatg gcgggcctgt accagcccag cgagggctac gtcgacgtcg
     2041 acggcctgga cctgcgccag gtcgacccgg ccgactaccg catgcgcgtg ggcttcgtct
     2101 cgcaggagcc gcgcctgttc aagggcacgt tgcgcgagaa cgtgatgatg ggacgcccgc
     2161 agctggacgc cggcaagctg gccgaggtgg ccaaggtgac gggcctggat cgcgtggtcg
     2221 cgggccatcc gcagggctgg gagctgtcgg tgggcgagat gggcggcctg ctgtccggcg
     2281 gccagcgcca gctcgtggcg ctcgcgcgct gcctgatcac caagccgcag atcctgctga
     2341 tggacgagcc caccagctcg atggacgcgc agtccgaagt gatgttcctg cgccagctca
     2401 aggaggccgc gggcacgcgc acgctgatca tggtcacgca tcgcccggcg gtgctggagc
     2461 tggtcgaccg cgtgatcgtg gtcgacggcg gcaagatcgt gctggacggc ccgaagggcg
     2521 cggtgctggc ggcgctgtcg ggcatcaagc cgcaggccca gccgcaggtg cagaaccggc
     2581 cggcgacgga acagccgatg cagcgccagc cggcgcccgc catggagagg gcgcaggccg
     2641 cctgacgcgt ctcccatggc caagttcacc ctcccgttca agctgccgtt cctgaagtcg
     2701 cccgagcccg cgctcgccgt gccgcagatg ggcggccgcg cgcgccgggg cgacgccgcg
     2761 ttcatgagcg gcgtgcgcgc ggcgcaggtc gtggaatcgg cgccgcacgt cacgtgggtg
     2821 ctgtacctga tggcggcggc gctggcgtcg gcattgacgt gggccgcggt cgcgcgcgtc
     2881 gacgaggtca ccaaggccga cgcgcgcgtc gtgcccgagg gccgcgagca ggtcatcgcc
     2941 agcctggaag gcggcatcct gcgcgagctg ttcgtcaagg aaggcgagca ggtcgaggag
     3001 ggccaggacc tcgcggaact cgaccccacg cgcttcaaga cgcagcaggc cgagggcgcc
     3061 gccaagatcc tcgcgatgaa ggcccagatc gcgcgcctga ccgccgaggc ctacaaccgt
     3121 ccgctcgtgt tcccgcccga ggtggaggcc gtgcccgacg tggcccaggg cgagcgcgac
     3181 tcgtacctcg cccgcaagcg cgccaacgac gacgcgctcg gctccaacca gcgcggcatg
     3241 gacatgctgc gccgcgaact gcagatgtcc gaggcgatgt ccgccaaggg gctgctgtcc
     3301 gaggtggaag tgctgcacct gcgccggcag gtcaacgaga tgtcgctgca gtcggaggac
     3361 cgcatcaacc gcttccgcca ggacgccagc tcggagctcg cgaagctgcg caccgacctg
     3421 gccgcgctgc aggagcagca ggccggccgc caggacgtgc tcgagcgcac gatgatcaag
     3481 tcgcccgtgc gcggcatcgt caagaacatc cgcatcaaca cgctcggcgg cgtgatcggc
     3541 ggcggcgcgc ccatcatgga gatcgtgccc atcggcaggc agtcgctcat cgaggcgcgc
     3601 gtcaagccgg gcgagatcgg cttcctgcag gtgggccaga ccgccaaggt caagctcgcc
     3661 gcctacgact tcgcgatcta cggcgccgtc gacggcaaga tcgagtcgat cagccccgac
     3721 gccctgggcg accccgagcg cgccgtgggc agcgccgatc cgacctacta tcgcgtgatg
     3781 ctgcgcgtgg acaacaacac gctgcacgag aagggcaaga cgctgccgct gctgccgggc
     3841 atgaccggct cggtggaggt gcgcatcggc gagcgctcgg tgctcaactt cctgctgcgg
     3901 ccgatgctca agtccaagga agcgttccgc gaacgctagc gatcacaaga gtccaggaga
     3961 tgtcatgacc ggtcgcatca ccgagatctt cggcggcttg caacagcggc acctgatcaa
     4021 cgagcaggtg gccgcctacc gcgactacac gcaggcggag gtcttcttcc acctggcgcg
     4081 cgagaagggc cagcgcgtcg agcagctgcg ccagcgcttc gccggccggc gcatcgagaa
     4141 gctgttcgtc ggcaacaacc tcgtgcccgc gggcttccag ctgccgggcg tcgagacggt
     4201 cgccctcgag aagaaccact tcgagcgcgc catgaagggc gagctgcagg acatggtggc
     4261 gcgctttgcc ggcggcatcg tgctcatcaa caacaacgac ctggccgtcg gcgaatcggc
     4321 gccgggctac gtgcgcgtgt tcgccggttc gcccgacacg atcttcctgg gctgggacta
     4381 cgacaaccac cactggatgg agtccagcct cgcgctggcc gcgcacaccg acctgtactt
     4441 cccggcgcac aacgagaaca tgtacctgct ctcgaagatc aacgcgctga ccagcgggcc
     4501 ggtcccgtgc gcctgcgtgc aatggagcca gtccttcctg tcggacaacc tgggcgagat
     4561 gctgcgccgc cagcgcagcg acgagccgct gggcatgcac ttcttctacc agcagttcgc
     4621 gttccgcaac cagacggtgg tcacgctcaa ccagaagtac aagtcgatcg gctttgccga
     4681 gcgctcgttc caggccaaga ccccgttcga ccgcctggcc gagtggatgg accacaaggc
     4741 gcactggatc atccccgtgc tcaacgacgt gccgatccgc atcttcgacg cgctggccag
     4801 cggcggcatc cccatcgtgc cggaatcgct gcgcttcctg gcccccattt ccggcctgcc
     4861 gcgcgacatg gtcgtgttct acggcccgga cgacatcttc aacccgcagg cggtcgtggc
     4921 gcgggcgctc gagctgttcg acgagggcgg gcaggggggc atcgccgccc gccaccgcca
     4981 cgcgctggcc caccaccacg gggcggaccg cctgcgccgg atggtcgcaa tggcccagga
     5041 aagactcgaa ttcgcgccgc ttatcggctg aacggcgacc cttcagtccg aacatgatcc
     5101 gttccatggt tctcacacac atggagcgcg atcaatgacc caggttcttg tcaccggcgg
     5161 cgccggcttc ctcggcagcc acctctgcga ccggctgatc gcgcgcggcg acgaagtgct
     5221 ctgcgtggac aacttctaca cgggcggcaa gggcaacgtg cgccacctga tgggcaacaa
     5281 gaacttcgag ctgatgcgcc acgacgtgac gttcccgctg tacgtcgagg tcgaccgcat
     5341 cttcaacctg gcctgcccgg ccagccccgt gcactaccag cacgacccgg tgcagaccac
     5401 caagacgagc gtgcacggcg ccatcaacat gctgggcctg gccaagcgca ccaaggcgcg
     5461 catcctgcag gcgtccacca gcgaggtcta cggcgacccg acgatgcacc cgcagcgcga
     5521 ggaatactgg ggcaacgtga acccgatcgg cccgcgctcg tgctacgacg aaggcaagcg
     5581 ctgcgccgag acgctgttct tcgactactg gcgccagcac cagctgcaga tcaagatcat
     5641 ccgcatcttc aacacctacg gcccgcgcat gcagcccgac gacggccgcg tggtgagcaa
     5701 cttcatcgtc caggcgctca agggcgagcc gatcacgctg tacggcgacg gcctgcagac
     5761 gcgcagcttc tgctacgtcg acgacctgat cgaggggatg atgcgcctga tggactccga
     5821 caaggccatc accggcccga tcaacgtcgg caacccggtc gagttcacga tgcgcgagct
     5881 ggccgagctg gtggtgcaga tcaccgggtc gaagtcgccg atcacgttcc gcccgctgcc
     5941 cacggacgat ccgaagcagc gtcgcccgga catcacccag gcgcaggaca agctcggctg
     6001 ggagcccaag gtgccgctgg ccgacggcct gaaggagacc atcgcctact tccgccgcac
     6061 gctggacttc acggccgatc tcgcgccggc caccgtgctg ccggcgatgc cgcccatccc
     6121 gaacatcgcc gccgcgctgg cgcgctgatc ctcgaatgtg tccgcggggc gccaggcgtc
     6181 ccggccgatc ccgacctgcc atgggaaccc cacagaacag atcgacctgg ccagcggcat
     6241 tgacgctggc cttcgcgctg ggcgcggccc tgctgtcgcc cgcggcgccg gccgcgaccg
     6301 cgcgccgggc gacgaccgcg ccggcgtccg ccgcgccggc cacgtcgtcc aacagccgct
     6361 gcatcagcga cgagagcacc gatccgccga cggtgccggc cttccgcacg gtgccggccg
     6421 actcgggcag cgccgcgcag gcgggcgccg tcgccgcgcc gcgcatgaac ctgctggcga
     6481 tggtgcgcag cgcgatccac cgcagcaacg cggtgggggc ggccaggctg ctgtccgagg
     6541 ccgccgcgat cgacatcgac gaggcgcacg ccgcctactg gccgcaggtg ggcatctcgg
     6601 gccaggtcgg cggcatcgac gccggcgcgc ccaactcccc gtcggtgcac ggcaagcagt
     6661 tccagggcgg catcaacgta tcggcgccgc tgtacgacgg cgggcacacg cgcgacatga
     6721 cggactaccg cacgcacctg gccgaggtcg cgaagttcgg cgaggtcagc acccaggaac
     6781 aggtggcgct gcagaccgtg tccctggcgc tggaccgcag ccgctacacc gtcgaggcgc
     6841 aggtctacga ccagtacgcc cgcaagatga gctgcctggt cgaggcgctc gagatgatcg
     6901 tctcggccga ccggggccgc accagcgaac tggtgcaggc gcgcaagacg cagcagcagg
     6961 ccgagctgca gcgcgaccag gtgctgtcgc agaagcggct gaccgagatc cgcctgcgcc
     7021 gcttcatcgg cgacgagctg ccgcccaccg agggcatcac ctcggtcatg ctggacaccc
     7081 cggcgctgcc cgacctgctg acgatggcct cgcgcgccgc cgagatccag tcgctggact
     7141 cgcaggccgc ggcgctcgac tcctacgtca aggccgtgct ggacggccag aagccgcagc
     7201 tgggcgtgct cgtggccgcc accaagtcgg ccggcgcggg cgactcgcgc tcggtctcgg
     7261 gcgggatcac gttcaactgg caggtcttca acgccgccaa caagtacacg accgaggccg
     7321 cccgcaagcg cgcggccgcg gcccgcatga accgcgagga cgcgctggaa gcccgcaagt
     7381 cccgcatggc cgaggtgcac gagcaggccg agcacgccat ggagcgcgcg cacagcgtca
     7441 tcgagatcct gcacaacagc gagctggtgc gcaacttcac gctgcagcag tggcagcagc
     7501 tcggccggcg ctcgctgttc gacgtgatgg ctgccgaggg cgaccactac aacatgcgcg
     7561 tccagtacgt cgacgcgctg tacgacaccc agcagtccaa cgcgctgctg tggtcgctgg
     7621 gcctgggcct ggcgaaccac ctggagtagc cgccaggctg ctgcgggaac cagagcaggg
     7681 ggacggcaag gcgctcctcg ggcggcgccg tgggcgccgc cttcgtcgtt ccggcgccga
     7741 tatgcggccc ccgatccggc cttttcgccc gatcggacga ggccttccgg cgcgaccatt
     7801 caggccattc ccataaagct ttccttggag tcgccgaatg cagccgtcca gccagctcgc
     7861 cccgtccgcc gcggcgcatc gcgctcccgg gtcgatgccg gcggatccga agagcgccgc
     7921 cctggccgcg gtcgccgcgc ccggcgccgc cagcatcgtc atcgaccgca acctgctgct
     7981 gcagggcatc gcgctgcaga acgagggcaa gctggaacag gcgcaggccg tcttcgagct
     8041 gtacctgacc cacatcccca acgagggcgt ggcgctgtat tcgctggccg tcgtgctgat
     8101 ggagcgcttc ggcgccgagc gcgcgctgcc cgtcgtcgag cgcggcgtgc aggtcgcctc
     8161 gaccttcccc ccgatctggt acgccgccgg ggccgtccag cagcgcctgg gcctgcgcga
     8221 cgcggccatc aggagctacg acaaggcgct cgagctcaag cccgactacg tcgaggcgct
     8281 gatcaacagc ggcacgctgc tgcgcgagca gctcgaccac cacgccgcga tcgatcgctt
     8341 caaccgcgcc ctggcgctca agccggacca cctgaacgcg atgtccaacc tcgcgatcat
     8401 gctcaccgag ttcaagcgct cggaagaggc ggtggcgatg ttcgagcggc tgctggcgat
     8461 caagcccgac ttcgagtacg gcgtcggctc gatctgctac gagcgcatgc acgcctgcga
     8521 ctggtcgcac ttcgattcgg acgtcgccaa gatcctcgaa ggcatcgagg ccggcaagcc
     8581 ggtcgccaag tcgctgggcg tgatgtccat caccgacgac gccgcggtcc atcaccgctg
     8641 cgccgagatc ttcgccgcgc accgctacac gccgcgccac gcgccgctgt ggacgggcca
     8701 gcgctaccgc cacgaccgca tccgcatcgc ctacgtctcg ccggacctgc gcgagcaccc
     8761 ggtgggccac ctgatggccg gcatcttcga gcgccatgac cgcaggcgct tcgagaccat
     8821 cgcgatctcc atcggcgcca acgacggcag ccgcctgcgc cagcgcatgg tcgcctgctt
     8881 cgaccacttc atcgacgcga agatgatgga gagccgcaag atcgccgagc tgatgcgttc
     8941 gatggaagtc gacatcgcca tcgaccttgc cggcttcacg gccgactcgc gcagtgaagt
     9001 gttcggcatg cgccccgcgc cggtgcaggt caactatctc ggctaccccg gaacgatggg
     9061 cacgcagtac atggactacg tcgtggccga ccgccacgtg atcccgcccg agcatgcccg
     9121 gcactacaac gagaaggtgg tgtacctgcc cgacgcgtac ctgccggccg cctcgggcct
     9181 gcaggtcgcc gagcgcacgc ccacgcgcgc cgaatgcggc ctgccggaag agggcgtcgt
     9241 gttctgctcg ttcaaccacg actacaagat cgccccgcac gtgttcgccg tttggatgaa
     9301 cctgctgcgc gaggtgcccg gctcggtgct gtggctgatg tcgcgcagcc cgctgtcgca
     9361 gaagaacctg cgcaaggaag cggccgcgcg cggtgtcgac cccgaccgcc tcgtgttcgc
     9421 gcagcgcgtg ccgcgcgtgg aggaccacct ggcgcgctac cgccaggccg acctgttcct
     9481 cgacacccat ccgtacaacg cccacaccac ctgcgccgac gcgctgatgg tcggcctgcc
     9541 ggtggtcacc tgcatgggcg gcgcgttccc gtcgcgcgtc gcgggcagcc tgctgcacgc
     9601 ggccggcgtc cccgagctgg tgacgtcgtc gctggccgac tacgaggcgc tcgcgctgca
     9661 gctggcgcgc gacccggcgc gcttggccgc gatcaagggc aagctggccg ccacgcgcac
     9721 cagcaccgcg ctgtgcgacg ccgacggctt cacgcgcaat ctcgaggcca tctacacgtc
     9781 gatgtggcgc cagtcgcagc tgggcggcgc gcgcgacgcg ctgagcgggg gcgcgcaatg
     9841 agcgccgcgg tcgcggacgc gcccgcggtc tcgcccgcca tcgctgccaa cgacgcgctc
     9901 gcgcgcggca acctgcagct ggccgactac ctgtgccgcg aggcgctgcg cgcggacgcg
     9961 aacgacgcgc tcgcgatgag cctgctgggc cacatggcct cgggcctgcg ccgcttccag
    10021 ctggccgagt cctggttcag ccgcgccgtc gcgctgcgcc cggcgtccgc cgagatcgcg
    10081 gcctggcgcg accgcgcccg cgagatggcg caggccgccc gcgcccacgc ggcatcggct
    10141 gcgcccaggg cgctgccgcg tccgaaatat ctcctggtca aggcctgggg ctacggcttc
    10201 tggtcggaca tggaccacgt cctgggccag ctgctgctgg ccgagctgac gggccgcacc
    10261 ccggtcgtgc actggggcgc gaacacgctg ttcggcgccg cgggcgtcgc caacgcgttc
    10321 gagctgtact tccgcccggt ctccggcgcc acgcaggacg gcctggccgc ctggggcgag
    10381 tcgttcttcc cggccaagtg gagcgcggcc aacctgcgcg acgagaacgt caacaagttc
    10441 cagggccccg gctcgcgcac ctccgcgctg cacctgctgg cgcgcgacga ggacgtcgtc
    10501 gtcagcgact tccacggcaa ggtcgtcgac ctgatgggct ggatcgagcc cgacagcccg
    10561 tggcatggcc tcgactacca ggccgtctac cgccgcctgt tcgccaagta cgtgcaactg
    10621 cagcccgcgc tgcaggcgcg cgtggacggg ttcgcgcgcg agcgcatggc cggacgccgc
    10681 tgggtggcag tgcacgtgcg cggcagcgac aagatcaccg agctgcgcga cctcgagctg
    10741 gtcaaccagc agtaccagca ccgcctctgg acgatgctgc aggccgatcc ggccatgggc
    10801 atcttcctgc tgaccgacag cgagcgcttc gcccgcgagt accgcgagaa gtacggcgac
    10861 cgcgtgctgg ccgccgacgt cgcgcgcagc ggcgacgcca ccggcgtgca ctacgccggc
    10921 cacgagggcc gcaagctggg cgaggaggtc gtgatggacg ccttcctggc cgcgcgctgc
    10981 gacgccttcc tgggcaacgg cgcgtccaac gtctccaccg ccatccgtca cctgaaggac
    11041 tggacgccgg gctcgttcga gctcatcggg ccggacttcc tggcgatgcc caaccagatg
    11101 ctgcatcgct ggtaaggtcg acgcgtgagc ccgcccggcc gcccggcggg cgaccaccgc
    11161 agtgcgcagc acggagaatc caattgactg ctcccatcgt catcgacggc gtcttcttcc
    11221 agatcggccg ctcgggcatc gcccgcgtgt ggcaatcgct gctgggcctg tgggcgggca
    11281 cgccgttcgg cgaccgcctg gtcgtgatgg accgcaaccg cacgatgccg cgcctgcccg
    11341 gcatccgcta cgtcgacgcc gccgcgctca actacaacga cctcgagagc
//
LOCUS       DATCRO010000755         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_796378, whole genome shotgun sequence.
ACCESSION   DATCRO010000755 DATCRO010000000
VERSION     DATCRO010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796378"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VIN75_20485"
     CDS             complement(<1..592)
                     /locus_tag="VIN75_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8976604.1"
                     /translation="MSKTFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNDKGGPA
                     PGGTCTNVGCIPSKALLQSSEHFEHAGHGFADHGITLDKLAIDVPKMLARKNSVVKQN
                     NDGILYLFKKNKISFFHGRGSFVKAVDGGYEIRVAGAAEETLVARQVIVATGSNPRAL
                     PGALFDEDKILSNDGALRIPAVPRTLGVIGSGVIGLE"
     gene            complement(605..1873)
                     /gene="odhB"
                     /locus_tag="VIN75_20490"
     CDS             complement(605..1873)
                     /gene="odhB"
                     /locus_tag="VIN75_20490"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY8976605.1"
                     /translation="MAIVEVKVPQLSESVTEATLLQWKKKVGDAVAVDEILIEVETDK
                     VVLEVPAPSAGVLTEIVQADGATVGAEQLIARIDTAATAGATAPAPAAAPAQAAPAPA
                     PAAAAAVTGGSKGDVAMPAAAKLLADNNLKATDVAGTGKDGRVTKGDVLGAVAAGAKP
                     AAAPVAAAPAPAAKAPLPAVAAPVVPTLGDRPEQRVPMSRLRARVAERLLQSQSQNAI
                     LTTFNEVDMAPVMALRKKYQEKFEKEHGVKLGFMSFFVKAAVAALKRYPVINASVDGN
                     DIVYHGYFDIGIAVGSPRGLVVPILRNADQMTFAEIEKKIAEFGQKAKDGKLSLEELT
                     GGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVVDGQIVVRPMNYLAMSYD
                     HRIIDGREAVLGLVAMKDALEDPSRLLFDI"
     gene            complement(1963..4821)
                     /locus_tag="VIN75_20495"
     CDS             complement(1963..4821)
                     /locus_tag="VIN75_20495"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY8976606.1"
                     /translation="MMEQSRSNSYLFGGNAPYVEEMYEAYLENPGSVPDTWRAYFDAL
                     QHVPAADGSDSRDVPHAPVIESFAQRAKANAFAVKASSTDLAIARKQVHVQSLIAAYR
                     FLGSRWADLDPLKRTERPRIPELDPAFYDLTEADMDITFSATNTYFSSNENMTLREIV
                     RALRETYCGTIGAEFMHITDPGEKRWWQERIEAIRGKPTFNAEQKKHILDRLTAAEGL
                     ERYLHTKFVGQKRFSLEGGESFIVSMDELVQRAGTKGVQEVVIGMAHRGRLNVLVNTL
                     GKMPKDLFAEFDHTAPEDLPSGDVKYHQGFSSDIATAGGPVHLSLAFNPSHLEIVNPV
                     AEGSVRARMDRRGDATGAQVLPVIVHGDAAFAGQGMVMETLALAQTRGYYTGGTVHLV
                     INNQIGFTTSDPRDMRSTLYCTDVVKMIEAPVLHVNGDDPEAVVLCTQLALDYRQQFN
                     KDVVVDIVCFRKLGHNEQDTPSLTQPLMYKKIAVHPGTRKLYGDKLVAQGVLGDTGPD
                     DMVKEFRAAMDAGRHTRDPVLTNFKGKYAVDWSPFLGKKWTDAADTAIPVTEFKRLAE
                     RITQVPSNFKVHPLVEKVLADRAAMGRGDINVDWGMGEHMAFASLVASGYPIRLSGED
                     SGRGTFTHRHAVLHDQNREKFDTGTYIPLENVTDKQAPFVVIDSILSEEAVLAFEFGY
                     ASSDPNTLVIWEAQFGDFVNGAQVVIDQFIASGEVKWGRANGLVMMLPHGYEGQGPEH
                     SSARLERFMQLAADTNMQIVQPTTASQIFHLLRRQMVRAFRKPLVIMTPKSLLRNKDA
                     TSPMSEFTKGEFRTVIPELDASIDAAKVKRVIACSGKVYYDLVKKRAERKSSDVAIIR
                     VEQLYPFPHKAFQAEMKKYPQATEIVWCQDEPQNQGAWFFVQHYIHENMLEGQKLGYA
                     GRAASASPAVGYAHLHQEQQIALLEQAFTKLKGFILTK"
BASE COUNT          748 a         1692 c         1851 g          950 t
ORIGIN      
        1 tctccaggcc gatgacaccc gagccgatga cgcccagcgt cctgggcacc gcgggaatgc
       61 gcagcgcgcc gtcgttggac aggatcttgt cctcgtcgaa cagcgcaccg ggcagcgcgc
      121 gcgggttcga gcccgtggcc acgatcacct gcctggcgac gagcgtctcc tcggcggcgc
      181 cggccaccct gatctcgtag ccgccgtcga ccgccttcac gaagctgccg cggccgtgga
      241 agaacgagat cttgttcttc ttgaacaggt agaggatgcc gtcgttgttc tgcttgacga
      301 cgctgttctt gcgcgccagc atcttgggca cgtcgatggc cagcttgtcg agcgtgatgc
      361 cgtggtcggc gaagccgtgg ccggcatgct cgaagtgctc ggacgactgc agcagcgcct
      421 tggacgggat gcagccgacg ttggtgcagg tgccgcccgg ggcggggccg cccttgtcgt
      481 tcttccattc gtcgatgcag gccgtgttga agcccagttg cgccgcgcgg atcgccgcga
      541 tgtagccgcc ggggccgccg ccgatgacga cgacgtcgaa agtcttgctc atgttgtcgc
      601 gtcctcagat gtcaaaaagc aggcgcgacg gatcttccag cgcatccttc atcgcgacca
      661 ggcccagcac ggcttcgcgg ccgtcgatga tgcggtggtc gtaggacatc gccaggtagt
      721 tcatcgggcg gacgacgatc tgcccgtcga ccaccaccgc gcgatccttg gtggcgtgca
      781 cgcccaggat ggccgactgc ggcgggttga tgatcggcgt ggacagcatc gagccgaacg
      841 tgccgccgtt ggagatggag aacgtgccgc cggtcagctc ctcgagcgac agcttgccgt
      901 ccttggcctt ctggccgaac tcggcgatct tcttctcgat ctcggcgaac gtcatctggt
      961 cggcgttgcg caggatgggc accaccaggc cgcgcggcga gccgacggcg atgccgatgt
     1021 cgaagtagcc gtggtagacg atgtcgttgc cgtcgaccga ggcgttgatg accgggtagc
     1081 gcttgagcgc ggccacggcg gccttgacga agaagctcat gaagccgagc ttgacgccgt
     1141 gttccttctc gaacttctcc tggtacttct tgcgcagcgc catgaccggg gccatgtcga
     1201 cttcgttgaa ggtggtcagg atggcgttct gcgactgcga ctgcagcagg cgctcggcga
     1261 cgcgggcacg caggcgcgac atcggcacgc gctgttccgg gcggtcgccc agcgtcggca
     1321 cgaccggcgc ggcgacggca ggcagcggcg ccttggccgc cggagccggg gcggcggcga
     1381 ccggagcggc cgcgggcttg gcgccggcgg cgaccgcgcc gaggacgtcg cccttggtga
     1441 cgcggccgtc cttgccggtg ccggcgacgt cggtcgcctt caggttgttg tcggccagca
     1501 gcttggcggc ggcgggcatc gccacgtcgc ccttggagcc gccggtgacg gcggcagcgg
     1561 cggccggcgc gggcgccggg gcggcctgcg cgggcgcggc agccggcgcg ggcgcggtgg
     1621 cgccggcggt ggcggcggtg tcgatgcgcg cgatcagctg ctcggcaccg acggtggcgc
     1681 cgtcggcctg cacgatctcg gtgagcacgc cggcggacgg cgcgggcacc tcgaggacga
     1741 ccttgtcggt ctcgacctcg atcaggatct cgtcgacggc gaccgcgtcg ccgaccttct
     1801 tcttccactg cagcagggtt gcttcagtca ccgactccga cagctgcgga accttcactt
     1861 caacgatggc catgaatatt ctctttgtgt gtacgtggac gcgagcccgg ccgtgagtcg
     1921 cccgttccct ccggaacgcg gcgccacggc cggcgcgatg tcttacttgg tcaggatgaa
     1981 gcccttgagc ttcgtgaagg cctgctccag cagcgcgatc tgctgctcct ggtgcaggtg
     2041 ggcgtagccc acggccggcg atgccgaggc ggcgcggccg gcgtagccga gcttctggcc
     2101 ctcgagcatg ttctcgtgga tgtagtgctg cacgaagaac caggcaccct ggttctgcgg
     2161 ctcgtcctgg caccacacga tctcggtggc ctgcgggtac ttcttcatct cggcctggaa
     2221 ggccttgtgc gggaacgggt acagctgttc cacgcggatg atcgcgacgt cgctcgactt
     2281 gcgttcggcg cgcttcttga ccaggtcgta gtacaccttg cccgagcagg cgatgacgcg
     2341 cttgaccttg gcggcgtcga tggacgcgtc gagctcgggg atcaccgtgc ggaattcgcc
     2401 cttggtgaac tccgacatcg gcgaggtggc gtccttgttc cgcagcagcg acttgggggt
     2461 catgatgacc agcggcttgc ggaaggcgcg caccatctgg cgacgcagca ggtggaagat
     2521 ctggctggcc gtggtgggct gcacgatctg catgttggtg tcggcggcca gctgcatgaa
     2581 gcgctccagg cgcgcggagc tgtgctccgg gccctggccc tcgtagccgt gcggcagcat
     2641 catcaccagg ccgttggcgc ggccccactt gacttcgccg gacgcgatga actggtcgat
     2701 cacgacctgc gcgccgttga cgaagtcgcc gaactgggct tcccagatca ccagcgtgtt
     2761 cgggtccgac gaggcgtagc cgaactcgaa cgccagcacc gcttcttcgg acaggatgga
     2821 gtcgatgacg acgaacggcg cctgcttgtc ggtgacgttc tccagcggga tgtaggtgcc
     2881 ggtgtcgaac ttctcgcggt tctggtcgtg cagcacggcg tggcggtgcg tgaacgtgcc
     2941 gcggcccgaa tcctcgcccg acaggcggat cgggtagccc gaggccacca gcgaggcgaa
     3001 ggccatgtgc tcgcccatgc cccagtcgac gttgatgtcg ccgcggccca tcgccgcgcg
     3061 gtcggccagc accttctcga cgagcgggtg caccttgaag ttgctcggca cctgcgtgat
     3121 gcgctcggcc aggcgcttga actcggtcac ggggatcgcc gtgtcggcgg cgtccgtcca
     3181 cttcttgccc aggaacggcg accagtcgac ggcgtacttg cccttgaagt tggtcagcac
     3241 cgggtcgcgg gtgtggcggc cggcgtccat cgcggcgcgg aactccttga ccatgtcgtc
     3301 cggccccgtg tcgcccagca cgccctgcgc gaccagcttg tcgccgtaga gcttgcgggt
     3361 gcccggatgc acggcgatct tcttgtacat cagcggctgc gtcaggctgg gcgtgtcctg
     3421 ctcgttgtgg cccagcttgc ggaagcagac gatgtcgacg accacgtcct tgttgaactg
     3481 ctggcgatag tcgagcgcga gctgcgtgca cagcacgacc gcttccgggt cgtcgccgtt
     3541 cacgtgcagc acgggcgcct cgatcatctt gacgacgtcg gtgcagtaca gcgtggagcg
     3601 catgtcgcgc gggtcggagg tggtgaagcc gatctggttg ttgatgacca ggtgcaccgt
     3661 gccgccggtg tagtagccgc gcgtctgcgc cagcgccagc gtctccatga ccatgccctg
     3721 gcccgcgaag gcggcgtcgc cgtggacgat gaccggcagc acctgcgcgc cggtggcgtc
     3781 gccgcggcga tccatgcggg cgcgcaccga gccttcggcc accgggttga cgatctccag
     3841 gtgcgacggg ttgaacgcca gcgacaggtg gacggggccg cccgcggtgg cgatgtcgct
     3901 ggagaagccc tggtggtact tcacgtcgcc cgagggcagg tcttccggcg cggtgtggtc
     3961 gaactcggcg aacaggtcct tgggcatctt gcccagggtg ttgaccagca cgttcaggcg
     4021 gccgcggtgg gccatgccga tgaccacttc ctgcacgccc ttggtgccgg cgcgctgcac
     4081 cagctcgtcc atcgacacga tgaagctctc gccgccttcg agcgagaagc gcttctggcc
     4141 gacgaacttg gtgtgcaggt agcgctcgag gccttcggcc gcggtcaggc ggtcgaggat
     4201 gtgcttcttc tgctcggcgt tgaaggtggg cttgccgcgg atcgcctcga tccgctcctg
     4261 ccaccagcgc ttctcgccgg gatcggtgat gtgcatgaac tcggcgccga tggtgccgca
     4321 gtacgtctcg cgcaacgcgc gcacgatctc gcgcagcgtc atgttctcgt tggacgagaa
     4381 gtacgtgttc gtcgccgaga acgtgatgtc catgtcggct tcggtcaggt cgtagaacgc
     4441 cggatcgagc tcggggatgc gcgggcgctc ggtgcgcttg agcggatcga ggtcggccca
     4501 gcgcgaaccc aggaagcggt aggccgcgat caacgactgg acgtggacct gcttgcgcgc
     4561 gatggccagg tcggtggaac tggccttgac ggcgaaggcg ttggccttgg cgcgctgcgc
     4621 gaaggactcg atcaccggcg cgtgcggcac gtcgcgggag tcggagccgt ccgcggcggg
     4681 gacgtgctgg agcgcgtcga agtaggcgcg ccacgtgtcc ggcacggagc cggggttctc
     4741 gaggtaggcc tcgtacatct cctccacgta cggcgcgttt ccgccgaaca ggtaggagtt
     4801 ggacctgctt tgttccatca tttcttcgct cactttcgcc ccggtttccg gagctacgat
     4861 gggttggaca accttccgcg gcccggctct accggtaggc ggattgcaaa cgccttgctg
     4921 ctaatcggat gccagcaagg ggacacagaa ggactattgg ggtacttcag accacgaatg
     4981 tagcacttac cctgactccg tgcctttgca ccgttgtccg gaaaacatgg actttctccg
     5041 cggatcgcca ggtgaaacgc cctgtcacag tcaggggagc atgtcccgcc aggtcatccg
     5101 actgcatccc gccgcccccg tgaaaccggc cgaggccgca ccgtgcaacg gctgcggcgt
     5161 ctgctgcgcg gcggagccct gcccggtcgg cgtgctcgtc agcggccgcc gctcgggcgc
     5221 ctgcgcggcg ctgtcgtgga a
//
LOCUS       DATCRO010000756         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_571142, whole genome shotgun sequence.
ACCESSION   DATCRO010000756 DATCRO010000000
VERSION     DATCRO010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571142"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2923
                     /locus_tag="VIN75_20500"
     CDS             <1..2923
                     /locus_tag="VIN75_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8976607.1"
                     /translation="ARIPFVSQMSSTSSLGSTSIVLQFDLDRNIDAAAADVQTAINAA
                     SAELPRDMPAPPSYSKVNPADPPILQLSAVSDTLPLTTVDDLVETRLVQSLSRVEGVS
                     DVSVGGRQKPAVRIQLDPMKLAAHGLSLEDARVALARLSGDNPKGVLNGDTRSYTLYT
                     NGQLATAAEWNDAVLASRDGVQLRVSDVGRAVLAAEDSTLSAWLDGHRAITLGVYRTP
                     GGNSIEIAERIKRKLPELIATLPPSVKVHVLADRTQTIRASLRDIEWTLLLNVVLVVA
                     VMYAFLRDVRATLIPAVTVPLSLLGACAVIWPLGYSLDNVSLLALAVAVGFVVDDSIV
                     MVENIRRRIDLGESPRRAALSGLRETGFTIVSISVSLIAVLLPLLLMGGMVGRLFREL
                     SVTLSITIVVSALVSLTVTPMLASLLLRPGRGRAGHSSANPAFDRLARGYARALDVAL
                     AHRRITLAIFVATAACAVALYVAVPKSFFPVQDTGLITGVSEAAQDISVEEMGRRQRA
                     LADVVRADPAVDHCMTAVGGSVFGGSTVNNGRWYVTLKPRDQRDATAAEVIRRLRPKL
                     AAVPGARLFLKPAQDISVGARVSPTQYQLTLQSADPLELAHWVPRVLSRLQAMSQLVD
                     VDADEQLAGTAVKIHIDRERAARLGISPQTVDATLNDAFGQREIARYFSQVSSYKVIL
                     EAPPALQRDPASLDRLFVTAPAIGELVPLRALAHWTTDEVAPLTIEHESRFPSVTISF
                     DLAAGVSLGDATAAIEAAKAELHLPATVQATFEGTARAFQSTLATMPLLIVAALVVSY
                     LSLGILYESTVHPWTILSTLPSAGVGAIAALWIAGFDFNLIGLVGVILLIGIVKKNGI
                     MLVDFAIGNTRRGGAASREAVREASVTRLRPILMTTLTAIFGALPLLLGAGTGSELRQ
                     PLGCSLIGGLLVSQLLTLFTTPVVYLYLDEFAARLRGRVDRLRRRASAGAAPPEPAAE
                     G"
     gene            2999..4840
                     /locus_tag="VIN75_20505"
     CDS             2999..4840
                     /locus_tag="VIN75_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEY8976608.1"
                     /translation="MTSLMTAVARDEGTEQARRAVGNDDFATPSARRIARAPSTTACL
                     ADVAAANATRLAVVDQRVRWSYRELLQRAAAVAARVREARGAGAEPVALLCPHAAPMI
                     AALLGVLGAGKAYVPLVPDEPAARLQAIVRACGARLVLAAPEFEPLARTLGIEVMVLD
                     DAAVADCPLGPRDGQSLSYVLHTSGTTGVPKGVLQNDRHVLHHAACYADALSLHADDR
                     MTLLPYYAFDASVMDIFATLLTGACLCIWDIRRDGVAGLDEWLARQEVTVWHSTPSVL
                     RTAFPHFVQSLALRWVVLGGEAATGHDAALARRHAGPACRLLNGLGPTECTTALQYVA
                     RPGLDDAASRLPVGRPVPGVRVLLLDPDGRESAAEGELVIESEHVALGYWGREDLSAE
                     RFAPAGDGSPRRRYRTGDVVRWNAEGQLEHLTRVDDQVKIRGVRIELGEVQAALSTHP
                     AIAHALVLALRDDPAREPVLVGYVVAAARGAAPDAAALRAHLAARLPAAMVPAAFVVL
                     DAFPLLANGKVDRRALPPPAEAEPATGEAPQTPLQKMLAAIWREVLQREVVHLDDNFF
                     ALGGESLRAMRVVAAVRARLEAELQLADVFEHPTLRRLASRMALYAD"
BASE COUNT          629 a         1915 c         1767 g          714 t
ORIGIN      
        1 cgccaggatc ccgttcgtca gccagatgtc atcgaccagc tcgctgggct cgacgtcgat
       61 cgtcctgcag ttcgacctgg accgcaacat cgacgccgcg gcggccgacg tgcagaccgc
      121 gatcaacgcc gcgtccgccg agctgccgcg cgacatgccc gcgccgccgt cctacagcaa
      181 ggtcaacccc gccgacccgc ccatcctgca actgagcgcg gtctcggaca ccttgccgct
      241 gacgaccgtc gacgacctcg tcgagacgcg cctcgtgcag agcctgagcc gcgtcgaggg
      301 cgtctccgac gtctcggtgg ggggccgcca gaagccggcg gtccgcatcc agctggatcc
      361 gatgaagctg gcggcccacg gcctgtcgct cgaggacgcg cgcgtggcgc tggccaggct
      421 gagcggcgac aaccccaagg gcgtcctcaa cggcgacacg cgctcctaca cgctctacac
      481 gaacggccag ctggccaccg ccgccgaatg gaacgacgcc gtcctcgcat cgcgcgacgg
      541 cgtgcagctg cgcgtgtcgg acgtcggtcg ggccgtcctc gcggccgagg acagcacgct
      601 gtcggcctgg ctggatggcc accgcgcgat cacgctgggc gtctaccgca cgccgggcgg
      661 caacagcatc gagatcgccg agcgcatcaa gcgcaagctg cccgagctga tcgccacgct
      721 gccgccgtcg gtcaaggtcc acgtgctggc ggaccgcacc cagaccatcc gcgcctcgct
      781 gcgcgacatc gagtggacgc tgctgctcaa cgtggtgctc gtggtcgccg tgatgtacgc
      841 gttcctgcgc gacgtgcggg ccacgctgat ccccgccgtc acggtgccgc tgtcgctgct
      901 gggcgcctgc gccgtcatct ggccgctggg ctacagcctg gacaacgtct ccctgctcgc
      961 gctggcggtc gcggtcggct tcgtcgtcga cgattcgatc gtcatggtcg agaacatccg
     1021 gcgccgcatc gacctgggcg aatcgccgcg gcgggccgcg ctgagcggcc tgcgcgagac
     1081 gggcttcacg atcgtctcga tcagcgtgtc cctgatcgcc gtcctgctgc cgctgctgct
     1141 catgggcggc atggtcgggc gcctgttccg cgagctgtcg gtgacgctgt cgatcaccat
     1201 cgtcgtgtcc gcgctggtgt cgctgaccgt cacgcccatg ctcgcgtcgc tgctgctgcg
     1261 gcccggccgc ggccgggcgg gtcactcgtc ggcgaacccg gcgttcgacc gcctcgcacg
     1321 cggctacgcg cgcgcgctcg acgtggcgct ggcccaccgg cgcatcacgc tggcgatctt
     1381 cgtggccacg gcggcgtgcg cggtggcctt gtacgtcgcc gtgcccaagt cgttcttccc
     1441 ggtgcaggac acgggcctga tcaccggcgt gtccgaggcg gcgcaggaca tctcggtcga
     1501 ggagatggga cgccgccagc gcgcgctggc agacgtcgtg cgcgcggacc cggccgtcga
     1561 ccactgcatg acggccgtgg gcggcagcgt gttcggcggc agcacggtca acaacggccg
     1621 ctggtacgtg acgctcaagc cgcgcgacca gcgcgacgcg acggccgcgg aggtcatccg
     1681 ccgcctgcgc ccgaagctgg cggcggtgcc gggcgcgcgc ctgttcctca agccggcgca
     1741 ggacatcagc gtcggcgcgc gcgtctcgcc gacgcaatac cagctcacgc tgcaaagcgc
     1801 cgacccgctc gaactcgcgc actgggtgcc gcgcgtgctg agccggctgc aggccatgtc
     1861 ccagctggtc gacgtggacg ccgacgagca gctcgccggc accgccgtca agatccacat
     1921 cgaccgcgaa cgcgccgcgc gcctgggcat ctcgccgcag accgtggacg cgaccctcaa
     1981 cgacgcgttc ggccagcgcg agatcgcgcg ctacttcagc caggtctcgt cctacaaggt
     2041 gatcctcgag gcgccgcccg cgctgcagcg ggacccggcc agcctcgatc ggctgttcgt
     2101 cacggcgccg gccatcggcg agctcgttcc gctgcgcgcg ctcgcgcact ggaccacgga
     2161 cgaggtggcg cccctgacca tcgagcacga gtcgcggttc ccgtcggtga ccatcagctt
     2221 cgacctcgcg gccggcgtct cgctgggcga tgccaccgcg gccatcgagg ccgccaaggc
     2281 cgaactgcac ctgccggcca ccgtccaggc cacgttcgag ggcacggcca gggccttcca
     2341 gagcacgctg gcgacgatgc cgctgctgat cgtcgcggcg ctggtggtgt cgtacctgtc
     2401 gctgggcatc ctgtacgaga gcacggtcca tccttggacg atcctgtcga cgctgccgtc
     2461 ggccggcgtc ggcgccatcg cggcgctgtg gatcgcgggc ttcgatttca acctcatcgg
     2521 cctggtcggc gtgatcctgc tgatcggcat cgtcaagaag aacgggatca tgctggtcga
     2581 cttcgccatc ggcaacacgc gccgcggcgg cgccgcctcg cgggaggcgg tgcgcgaggc
     2641 cagcgtgacg cgcctgcgcc cgatcctgat gacgacgctg accgcgatct tcggcgcgct
     2701 gccgctgctg ctgggcgccg gtaccggctc ggaactgcgc cagcccctgg gctgctcgct
     2761 gatcggcggc ctgctggtca gccagctgct gacgctgttc acaacgccgg tggtctacct
     2821 ctacctcgac gaattcgcgg cgcgcctgcg tggtcgcgtc gaccgcctgc gtcgccgcgc
     2881 ctcggcgggg gccgcgccac ccgaaccggc tgccgaaggc tgacgccgcc gcacggcccc
     2941 ttccacacgg cccgccatcg acgaggcgcg cccgatgcac caaacccaag gacgacaaat
     3001 gaccagcctg atgaccgccg tggcgcgcga cgaaggtacc gagcaggccc gccgcgccgt
     3061 cggcaacgac gacttcgcca ccccgtcggc gcgccggatc gcccgcgcgc cctcgaccac
     3121 cgcctgcctg gccgacgtcg cggcggccaa cgcgacgcgc ctggcggtcg tggaccagcg
     3181 cgtgcgctgg agctaccgcg agctgctgca gcgcgccgcc gcggtggccg cgcgcgtgcg
     3241 ggaggcgcgc ggcgccggcg cggaaccggt ggcgctgctg tgcccgcacg ccgccccgat
     3301 gatcgcggcg ctgctgggcg tgctgggcgc gggcaaggcg tatgtgccgc tggtgcccga
     3361 cgagccggcc gcgcggctcc aggccatcgt gcgcgcctgc ggcgcccggc tcgtcctcgc
     3421 cgcaccggag ttcgagccgc tggcccgcac gctgggcatc gaggtgatgg tgcttgacga
     3481 cgcggccgtc gccgactgcc cgctcggtcc gcgcgacggg caatcgctgt cgtacgtgct
     3541 gcacacctcc gggacgacgg gcgttcccaa gggcgtgctg cagaacgacc gccacgtgct
     3601 gcaccacgcc gcctgctacg ccgacgcgct gtcgctgcac gccgatgacc gcatgacgct
     3661 gctgccgtac tacgccttcg acgcctcggt gatggacatc ttcgccacgc tgttgacggg
     3721 cgcctgcctg tgcatctggg acatccgccg cgatggcgtc gcgggcctgg atgaatggct
     3781 cgcgcgccag gaggtcacgg tgtggcattc gacgccgagc gtgctgcgca cggcgttccc
     3841 gcacttcgtg cagtccctgg cgctgcgctg ggtcgtgctg ggcggcgagg ccgcgaccgg
     3901 ccacgacgcg gcgctcgcgc ggcgccacgc cggcccggcc tgccgcctgc tcaacggcct
     3961 gggcccgacc gagtgcacga cggcgctgca gtacgtcgcc cggcccgggc tggacgacgc
     4021 ggcgtcgcgc ctgccggtcg ggcgcccggt tcccggcgtg cgcgtgctgc tgctcgaccc
     4081 ggacggccgc gagtccgccg ccgagggcga gctggtgatc gagagcgagc acgtcgcgct
     4141 cggctactgg ggccgcgagg acctgtccgc cgagcgcttc gcgccggccg gcgacggctc
     4201 gccacgccgc cgctatcgca ccggcgacgt cgtgcgctgg aacgcagagg gccagctcga
     4261 acacctgacg cgggtcgacg accaggtcaa gatccgcggc gtgcgcatcg agctcggcga
     4321 ggtccaggcc gcgctgtcca cgcatccggc gatcgcgcac gcgctcgtgc tcgcgctgcg
     4381 cgacgatccg gcgcgcgagc ccgtcctggt cggctacgtc gtcgcggcgg cgcgcggcgc
     4441 ggcgcccgat gccgcggccc tgcgcgccca cctggcggcg cgcctgcccg cggccatggt
     4501 gccggccgcg ttcgtggtcc tcgacgcgtt cccgctgctg gccaacggca aggtcgaccg
     4561 ccgcgccctg ccgccgccgg ccgaagcgga accggcgacg ggcgaggcgc cgcagacccc
     4621 gctgcagaag atgctcgccg cgatctggcg ggaggtgctg cagcgggagg tcgtgcacct
     4681 ggacgacaac ttcttcgcgc tcggcggcga gtcgctccgc gcgatgcggg tggtcgccgc
     4741 cgtccgcgcc cgcctggaag ccgagctgca gctggccgac gtgttcgagc acccgacgct
     4801 gcgccggctg gccagccgca tggcgctgta cgccgattga cgagcctcgc ctcccgccct
     4861 tgaccgcggc cgcgcgcctg caccgtccca cttcttcccg tcctgttgcc atgaacccca
     4921 gctccgacac cgtcgacggc ctcgtcgccg atctctgcca cctcgtcggc gtggcctgcg
     4981 gccgcagcgg cctggcgccc ggcgatgccc tgggcgacag cctgg
//
LOCUS       DATCRO010000757         9017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1021033, whole genome shotgun sequence.
ACCESSION   DATCRO010000757 DATCRO010000000
VERSION     DATCRO010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021033"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VIN75_20510"
     CDS             complement(<1..1464)
                     /locus_tag="VIN75_20510"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY8976609.1"
                     /translation="MRDDIAVPGASPAADLPQAFDASFAQQGLWILDQFGQAPGLYNI
                     PAVWRLRGALRVAALEQGLAEIVRRHEVLRTSLAQVDGRLCQMVHDAAPLVLERLDVP
                     DGPDAAVRAREIAERHAALPFDLGRAPLVRASLVRIAADDHLFALTLHHAVADAWSVG
                     VLMRELAALYTASAAGAPSPLPALEIQYADYAVWQRDWLQGEVLAEQLAYWTRTLRGA
                     PAGLALPADRPRPPVMSHRGDTLSFDLERETVAGLRRLARESRATLFMVFAAALSALL
                     ARWTGQDDLSIGYPVANRGEAETEALIGMFTNTLVLRTRPRPGIDFATLLAAVRESVL
                     DADAHQQVPFEKIVEALQPERRLGESPLFQVMLSLDNTGLERLRLGDLAIEPLPAAGG
                     TAKFDLSVDLLEDGDRVRGEFEYATDLFDRATIEQLARSWRCLLEAVAASPHMPLDRL
                     PLAERGAAAPPAEAAPAAPPFEPLLARLARIAAQAPDA"
     gene            complement(1467..1664)
                     /locus_tag="VIN75_20515"
     CDS             complement(1467..1664)
                     /locus_tag="VIN75_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HEY8976610.1"
                     /translation="MNETSMPYLVVKNDEEQYSIWAADLPLPPGWQAQGDARDKSACL
                     AWIDEHWSDMRPASLRRHAAD"
     gene            complement(1688..4852)
                     /locus_tag="VIN75_20520"
     CDS             complement(1688..4852)
                     /locus_tag="VIN75_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8976611.1"
                     /translation="MILPLTAGQHGLWFLCQSDARTSAAYNVVLAQTVDGELDVDLLR
                     RAWTRLLERHPLLGARIAVADFEPRFVVDETPRVDVVTVDGDLAAVAHAASANALDLE
                     RGPLHRVSVVRDAGGRTSGLVIAIAHVVFDGGSVAPLMRELGQAWDALARGEQMRGDA
                     APPSQAALAEAEAAFLRSDAGRQRVAGLVQRLAGISALPVPPIAGRAGPGGAGVHEAA
                     TCEATLPEREVARVRQLAARERTTPAAVYLAAFEILVWQYAGQASFTVGVPTSLRDET
                     TAGAVGYLANLGILRASIEPRQTVRAVIATASAQVLDLVDDARVPFPVLVRALKKAGE
                     EAARLQPRLGFNCQPAPPLSLRLGSASLHAVGTTQAYAKDRLALDVEEGAQTSRIALL
                     FDRASIDASTAADMLAQFTALLAQMCDDADLPAGRLARMTAARQAGLLAACNPPAGAP
                     VLADADVVDLFERRARSTPGRPALVADGAHWNYGELHARAASLARALRDAGVRSEHAV
                     AVRLPRGAQQVVALLANLMAGGVHVPVDLEQPADRLQALIADTRPIVLVTDGTLPHDP
                     ALAALPAIRLDALADTPADGREPAPARRAPGQLAYCIQTSGSTGRPKTVGVSAAALGR
                     HVAACIGHYAMSADDRVLQVAASHLDPAIEQTLVALCSGACLHVRGQATWSPEDLVAA
                     IATHGITVADVPTAYWKSYADTGFAALRPLPLRLLIIGGEAAPAATLPSPPLPFDWLN
                     AYGPTEATITATTWRGGSGVDVGQPYLPIGRPLGHTRAYVLDEHLQPMPPGVRGELFL
                     AGPQLARGYVGMPALTAASFLPDPFGPPGSRLYRTGDLARLGEDGQLEFLGRIDRQVK
                     IRGFRVELGDVEAALHRVPGVQAAVVTPLADAAGATSALVAYVVAPGCTPADLRAQLA
                     LPDHMRPAHWVALDALPIGANGKVDRAALPAPASAQADAAGFAAPASELERSIAAVWC
                     EVLQLDRVGLHDNFFALGGHSLLATQIIARLAERLGRAVKLRDLLDAPSVGALAGRLA
                     SDAGAAAVQRPRLVRQARTANAAS"
     gene            complement(4888..6180)
                     /locus_tag="VIN75_20525"
     CDS             complement(4888..6180)
                     /locus_tag="VIN75_20525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976612.1"
                     /translation="MNQALSMRAGGALPLVLTRGVSAFGGALMAFAIDVWIFRRTGSY
                     PTFALLALLTYLPNLVLAPFAGMLVDRCDKKRVLVGCELASLATILFALVAYRRDALG
                     IGAAAVVILSQSVVGHVRWTAMGVTVTLLTTPATRAGINGVQQSIAGVLDTCAPILGA
                     LAFESCGVAGILSFALLGSAVGLAGMWTVDGRRLRSAAQPAPREASLWREATFGLRWI
                     AGQPMLARLLLFIMGYNLAGAVFSVTFTPYLLSHAPVAVLGVASALEGGSALVVGFAL
                     IRIGQRVPPIAQVVGGALAFGLAMIAWGWAHGAIALCALAFCCGCLTSLIVASLQTVW
                     QNGVPVEIQGRVFAARRMVSFLLVPLAVLASVPLSQHVFAPCLQSSALAAGVWGQGQG
                     GALGMMLSTLGAALAAGSAWFARRHRLQAHPAAAPLTP"
     gene            complement(6177..8786)
                     /locus_tag="VIN75_20530"
     CDS             complement(6177..8786)
                     /locus_tag="VIN75_20530"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEY8976613.1"
                     /translation="MHFLDDATEHPPVADARPRWPAHLTAPSAAGWALWRDCMLRSAG
                     FPADMLRPLVDESCSQLADRLDEARAMRDAIVREAVACIGARIDAHRGDEAAEGQVRQ
                     WQREIKALRRGDVAGALPASLGAPLHERLALAARRCDELQAAWAVSLAAANDAFTAVA
                     ARLAADPLFREAVTWQNHAAVTTALDPLAAGAPMSGSKRRQREALVAGYLQRYALKND
                     TIGFFGPMTWARLVADPREARYDAGSGLVRRRSVRFEDWPIATLAADMARDERLLPWL
                     IPRRQPFLEVQGERLRLPGGGSAALAAGEARLLAACDGRTAAGDIARRLAADPFGEFD
                     DPAEVLDGLRRLERAGRIHLGLQISSCDPRPELALRREFARIGDVALRAELSDRLGLF
                     EAARDEIARSAGDADRLHGAMRRLDTLFDAQTGAGARRRPGEAYGGRGLAYEDCHRDL
                     DVRLGEAALQPLRGALDFVLASSRWYIGQAAARFHDRFAAIVESLSQDAEDVRFADFW
                     LQAQCVLFAEPDIVADLSDSLRERWERVLEPFVSPGRHRCEIASGAIREVMAQAFPAD
                     QPAWVMARHQCPDLMFCAESPQALQRGDYLAVLGEVHIGGNTVATNLFVSQHPRPAEM
                     LHALAQDLDAPYVCPKLSPETSRTPIRTQFVEDPDACREIMFSHGLVPANPATALDVA
                     ALDVGFGADGRLVARQRGGHWQAPLLDVLGDFLFLRVLNEFGVTRRRRHTPRLTIDRL
                     VVQRERWIFDAAELAALAATPAGDVFLAARRWARTAGLPAQVFVKLTWEDKPFHVDFR
                     SPVLVQMFVRLLHGGAESGRDAGGSVTLTEMLPSADELWLHDAAGRRYTSELRLVCIH
                     RDDVAGATGQDAR"
     gene            complement(8821..>9017)
                     /locus_tag="VIN75_20535"
     CDS             complement(8821..>9017)
                     /locus_tag="VIN75_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8976614.1"
                     /translation="AGLAPAGAPDADDDLFDRGFHSMLLVRLSTRLHEALGVRLALRE
                     LVQLRTPRALAGHVDALLAR"
BASE COUNT         1242 a         3204 c         3525 g         1046 t
ORIGIN      
        1 cgcgtcgggc gcctgcgcgg cgatgcgcgc gaggcgggcc agcagcggtt cgaacggcgg
       61 cgccgccgga gcggcctcgg ccggcggtgc agcggcgccg cgttcggcca gcggcagccg
      121 gtccagcggc atgtgcggcg aggcggcgac ggcctcgagc aggcatcgcc aggagcgcgc
      181 cagctgctcg atcgtggcgc ggtcgaacag gtcggtcgcg tactcgaact cgccgcgcac
      241 ccgatcgccg tcctcgagca ggtcgacgct caggtcgaac ttggcggtgc cgccggcggc
      301 gggcagcggc tcgatggcca ggtcgcccag ccgcagtcgt tccaggcccg tgttgtccag
      361 cgacagcatg acctggaaca acggcgattc gccgagccgg cgctccggct gcaaggcctc
      421 gacgatcttc tcgaagggga cctgctggtg ggcgtcggcg tcgagcaccg attcgcgcac
      481 cgcggccagc agcgtcgcga agtcgatccc gggccgcggg cgcgtccgca gcaccagcgt
      541 gttggtgaac atgccgatca gcgcttcggt ctcggcctcg ccgcggttgg ccacggggta
      601 gccgatgctg aggtcgtcct ggccggtcca acgggccagc agcgcggaca gcgcggcggc
      661 gaataccatg aacagcgtgg cgcgcgactc gcgcgcgagg cgccgcaggc cggcgacggt
      721 ctcgcgctcc agatcgaagg acagggtgtc gccgcggtgg ctcatgacgg gcgggcgcgg
      781 tcgatccgcg ggcagcgcga ggccggcggg cgcgccgcgc agcgtgcgcg tccagtaggc
      841 cagctgctcg gcgagcacct cgccctggag ccagtcgcgt tgccagaccg cgtagtccgc
      901 gtactggatc tcgagcgcgg gcagcggcga cggcgcgccg gccgccgatg cggtgtacag
      961 cgccgcgagt tcgcgcatca gcacgccgac ggaccaggcg tccgcgaccg cgtggtgcag
     1021 ggtcagcgcg aacaggtggt cgtccgcggc gatgcgcacg aggctcgcgc gcacgagcgg
     1081 cgcgcggccg aggtcgaacg gcagcgccgc gtggcgttcg gcgatctcgc gtgcgcgcac
     1141 ggccgcatcg gggccgtcgg gcacgtccag gcgctcgagg acgagcggcg ccgcgtcgtg
     1201 gaccatctgg cacaggcggc cgtccacctg cgcgagcgag gtgcgcagca cttcatgccg
     1261 ccgcacgatc tcggccaggc cctgttccag cgccgcgacg cgcagggcgc cgcgcagccg
     1321 ccagacggcg gggatgttgt agagcccggg cgcctgcccg aactggtcga ggatccacag
     1381 gccctgctgc gcgaacgacg cgtcgaacgc ctgcggaaga tccgccgcgg ggctcgcgcc
     1441 gggcactgcg atgtcgtcgc gcatggtcag tccgcggcgt gccggcgcag gctggcggga
     1501 cgcatgtccg accagtgttc gtcgatccag gccaagcacg cggacttgtc ccgcgcgtcg
     1561 ccctgcgcct gccagccggg tggcagcggc aggtcggcgg cccagatcga atattgctct
     1621 tcgtcgttct tgacgacgag gtagggcatc gaggtctcgt tcatggcgtt gcagttccgg
     1681 tgatgtgtca ggaggcggcg ttggccgtgc gggcctggcg caccaggcgc ggccgttgga
     1741 cggccgcggc gcccgcgtcg ctcgcgaggc gcccggcgag cgcgccgacg ctcggggcgt
     1801 cgagcaggtc gcgcagcttc accgcgcgac ccaggcgctc ggccaggcgc gcgatgatct
     1861 gggtggcgag cagcgagtgg ccgccgaggg cgaagaagtt gtcgtgcagg cccacgcgat
     1921 cgagctggag cacctcgcac cagacggccg cgatgctgcg ttcgagctcg ctggcgggcg
     1981 cggcgaagcc ggccgcgtcg gcctgcgcgc tggccggcgc gggcagcgcg gcgcggtcga
     2041 ccttgccgtt ggcgccaatg ggcagggcgt ccagcgcgac ccagtgcgcg ggccgcatgt
     2101 gatcgggcag cgccagttgc gcgcgcaggt cggccggcgt gcatccgggc gccaccacgt
     2161 aggcgaccag cgcgctcgtg gcgccggcgg cgtcggccag cggggtgacc acggcggcct
     2221 gcacgcccgg cacgcgatgc agcgccgcct cgacgtcgcc gagctcgacg cggaagccgc
     2281 ggatcttgac ctggcgatcg atgcggccga ggaactcgag ctggccgtcc tcgccgagcc
     2341 gcgcgaggtc tccggtgcgg tacaggcgcg atccgggagg accgaacggg tcgggcagga
     2401 agctcgcggc cgtcagcgcc ggcattccca cgtagccgcg cgccagttgc ggcccggcca
     2461 ggaacagctc gccgcggacg ccgggcggca tgggctgcag gtgctcgtcg agcacgtagg
     2521 cgcgggtgtg cccgagcggc cggccgatcg gcaggtaggg ctggccgacg tcgacgcccg
     2581 aaccgccgcg ccaggtggtg gccgtgatgg tggcttcggt cgggccatag gcgttgagcc
     2641 agtcgaaggg caacggcggc gacggcagcg tggcggcggg cgcggcctcg ccgccgatga
     2701 tcagcaagcg cagcggcagc gggcgcaagg cggcaaagcc ggtgtcggcg taggacttcc
     2761 agtaggccgt cggcacgtcg gccacggtga tgccatgcgt ggcgatcgcg gcgacgaggt
     2821 cctccggcga ccaggtcgcc tggccccgca cgtgcaggca cgcgccgctg cacagcgcga
     2881 ccagcgtctg ctcgatggcc gggtccaggt gcgacgccgc gacctgcagc acgcgatcgt
     2941 cggcgctcat cgcgtagtgg ccgatgcagg cggcgacgtg gcgtccgagt gcagcggcgg
     3001 acacgccgac ggtcttgggg cggccggtgg aacccgaggt ctggatgcaa taggcgagct
     3061 gtcccggcgc gcggcgggcg ggggccggct cgcgtccgtc ggcgggcgtg tccgccagcg
     3121 cgtcgaggcg gatggccggc aacgcggcca gcgcgggatc atgcggcagc gtgccgtcgg
     3181 tcacgaggac gatggggcgc gtgtcggcga tcagggcctg caggcggtcg gcgggctgct
     3241 cgaggtcgac cgggacatgc accccgcccg ccatcaggtt ggccaggagc gcgacgacct
     3301 gctgggcgcc gcgcggcagg cgcacggcca ccgcgtgttc cgatcgcacg ccggcgtcgc
     3361 gcagcgcgcg ggccaggctc gcggcgcggg cgtgcagctc gccatagttc cagtgggcgc
     3421 cgtcggcgac cagcgccggc cggccgggcg tgctgcgcgc gcgtcgctcg aacaggtcga
     3481 ccacgtcggc gtcggccagc accggcgcgc cggccggcgg attgcacgcc gccagcaggc
     3541 cggcctgccg cgccgccgtc atgcgtgcga ggcggcccgc cggcaggtcg gcgtcgtcgc
     3601 acatctgcgc aagcagcgcg gtgaactgcg ccagcatgtc cgcggccgtc gaggcgtcga
     3661 tgctcgcgcg gtcgaacagc agcgcgatgc gggacgtctg cgcgccttcc tcgacgtcga
     3721 gtgcgaggcg atccttggcg taggcctgcg tcgtccccac ggcgtgcagc gaggccgagc
     3781 ccagccgcag cgacagcggc ggggccggct ggcagttgaa gcccagccgc ggctgcaggc
     3841 gggcggcctc ctcgccggcc ttcttcaagg cgcgaaccag cacggggaac ggcacgcggg
     3901 cgtcgtcgac caggtcgagc acctgggcgg acgccgtcgc gatcacggca cgcacggtct
     3961 gtcgcggctc gatgctggcg cgcaggatgc ccaggttggc gaggtagccg acggcgccgg
     4021 cggtggtctc gtcgcgcagg ctggtcggca cgccgacggt gaacgacgcc tggccggcgt
     4081 attgccacac caggatctcg aacgccgcca ggtatacggc ggccggcgtc gtgcgctcgc
     4141 gcgcggcgag ctggcgcacg cgcgcgacct cgcgctcggg cagcgtcgcc tcgcacgtgg
     4201 cggcctcatg gacgccggcg ccacccgggc cagcgcggcc cgcgatcggc ggcacgggca
     4261 gcgccgagat gccggccagc cgctgcacga gtccggcgac gcgctggcgg ccggcgtcgc
     4321 tgcgcaggaa cgcggcttcg gcctcggcca gcgcggcctg gctgggcgga gcggcgtccc
     4381 ctcgcatctg ctcgccacgc gccagcgcgt cccaggcctg gccgagctcg cgcatcagcg
     4441 gcgcgaccga gccgccgtcg aagaccacgt gggcgatggc gatcaccagt cccgacgtgc
     4501 gtccgccggc gtcgcgcacc acgctgacgc gatgcagcgg gccgcgttcg aggtccagcg
     4561 cgttggcgct cgcggcatgc gcgaccgcgg ccaggtcgcc gtcgaccgtg acgacgtcga
     4621 cgcgcggcgt ctcgtcgacg acgaagcgcg gctcgaagtc ggccacggcg atgcgcgcgc
     4681 cgagcagcgg atggcgctcg agcaggcgcg tccaggcgcg gcgcagcagg tcgacgtcga
     4741 gctcgccgtc gaccgtctgc gcgagcacga cgttgtaggc ggcgctagtg cgcgcgtcgg
     4801 actggcacag gaaccagagg ccgtgctggc cggcggtcag gggaaggatc atggtggcga
     4861 tggcgggacg ttggagggga cctggcgtca gggcgtgagc ggcgcggcgg cgggatgcgc
     4921 ctggaggcgg tggcggcgcg cgaaccaggc gctgccggcg gcgagcgcgg cgccgagcgt
     4981 cgacagcatc attccgagcg cgccgccctg gccttgtccc cacacgcccg cggccagggc
     5041 cgacgattgc aggcagggcg cgaacacgtg ctgcgacagc ggcaccgacg cgagcacggc
     5101 cagcggcacg agcaggaacg acaccatccg gcgcgcggcg aacacgcggc cctggatctc
     5161 gaccggcacg ccgttctgcc agaccgtctg caacgaggcg acgatcagcg aggtgaggca
     5221 gccgcaacag aaggccaggg cgcacagggc gatcgcgcca tgcgcccagc cccaggcgat
     5281 catcgccagc ccgaaggcca gggcgccgcc gacgacctgc gcgatcggcg gcacgcgctg
     5341 cccgatgcgg atcagcgcga agccgaccac cagcgccgat ccgccctcca gcgccgatgc
     5401 gacgccgagc acggccaccg gcgcgtggct cagcaggtag ggcgtgaagg tcacgctgaa
     5461 caccgcgccg gcgaggttgt agcccatgat gaacagcagc agccgcgcga gcatgggttg
     5521 gccggcgatc cagcgcaggc cgaaggtcgc ctcgcgccac aggctggcct cgcggggcgc
     5581 cggctgcgcg gcggaccgca ggcgccggcc gtcgaccgtc cacatgcccg ccaggccgac
     5641 cgccgatccc agaagcgcga acgagaggat gccggccacg ccgcagctct cgaacgcgag
     5701 cgcgcccagg atcggcgcgc aggtgtcgag cacgccggcg atggactgct gcacgccgtt
     5761 gatgcccgcg cgcgtggcgg gcgtcgtgag cagcgtgacc gtcacgccca tggccgtcca
     5821 gcgcacgtgg ccgaccaccg attgcgacag gatcacgacg gccgcggcgc cgatgcccag
     5881 cgcgtcgcgg cggtaggcca ccagcgcgaa gaggatcgtg gccagcgacg ccagctcgca
     5941 gccgaccagc acccgcttct tgtcgcagcg gtcgaccagc atgccggcga acggcgccag
     6001 caccaggttg ggcaggtagg tcagcagggc cagcagggca aacgtcggat aggagccggt
     6061 gcgccggaag atccagacgt cgatggcgaa ggccatcagg gcgccgccga acgcgctcac
     6121 gccgcgcgtc agcacgagcg gcagcgcgcc gccggcgcgc atggacaagg cctggttcat
     6181 cgcgcgtcct gccccgtggc gccggcgacg tcgtcgcggt ggatgcacac cagccgcagc
     6241 tcgctggtgt agcgccggcc ggcggcgtcg tgcagccaca gttcgtcagc ggacggcagc
     6301 atctcggtga gcgtcaccga gcccccggca tcgcggccgg attcggcgcc gccgtgcagc
     6361 aggcgcacga acatctgcac cagcaccggg ctgcggaagt cgacgtggaa aggcttgtcc
     6421 tcccacgtca gcttgacgaa cacctgcgcc ggcaggccgg ccgtgcgcgc ccagcggcgt
     6481 gccgccagga agacgtcacc ggccggcgtg gcggccagcg cggcgagctc cgcggcgtcg
     6541 aagatccagc gctcgcgctg gacgaccagg cgatcgatcg tgagccgtgg cgtgtggcga
     6601 cgccgccggg tcacgccgaa ttcgttgagc acccgcagga acaggaagtc gcccaggacg
     6661 tcgagcagcg gggcctgcca gtggccgccg cgctgccgcg cgaccagccg gccgtcggcg
     6721 ccgaagccga cgtcgagcgc cgcgacgtcg agcgccgtcg ccgggttggc gggcaccagg
     6781 ccgtgcgaga acatgatctc gcggcaggcg tccggatcct ccacgaactg cgtgcggatc
     6841 ggcgtgcgcg acgtctcggg gctgagcttc gggcagacgt agggcgcgtc caggtcctgc
     6901 gcgagcgcgt gcagcatctc cgcgggccgc gggtgttgcg acacgaacag gttggtcgcg
     6961 acggtgttgc cgccgatgtg cacttctccc agaacggcca ggtagtctcc gcgctggagc
     7021 gcctgcggcg actcggcgca gaacatcagg tctgggcact ggtggcgcgc catcacccag
     7081 gccggctggt cggcggggaa ggcctgcgcc atcacctcgc ggatcgcgcc cgaggcgatc
     7141 tcgcagcgat gccggcccgg cgagacgaaa ggctccagca cgcgctccca gcgttcccgc
     7201 agcgagtcgg acaggtcggc gacgatgtcc ggctcggcaa agaggacgca ctgggcctgc
     7261 agccagaagt ccgcgaagcg cacgtcctcg gcgtcctggg agagcgactc gacgatcgcg
     7321 gcgaagcggt cgtggaagcg cgcggcggcc tggccgatgt accagcgcga gctcgcgagg
     7381 acgaagtcca gcgcgccgcg cagcggctgc agggcagctt cgccgagccg cacgtcgagg
     7441 tcgcgatggc aatcctcgta cgcgagcccg cgtccgccgt aggcctcgcc cggccggcgg
     7501 cgtgcgccgg cgccggtctg cgcgtcgaac agcgtgtcca ggcgacgcat cgcgccgtgg
     7561 aggcgatccg cgtcgccggc ggatcgcgcg atctcgtcgc gcgccgcttc gaacagtccc
     7621 aggcggtcag acagctcggc ccgcagcgcg acgtcgccga tgcgcgcgaa ctctcgccgc
     7681 agcgcgagct cggggcgcgg gtcgcagctg gagatctgca ggcccaggtg gatccggccc
     7741 gcgcgctcga ggcgccgcaa gccgtccagc acctcggccg gatcgtcgaa ttcgccgaaa
     7801 gggtcggccg cgagccggcg cgcgatgtca ccggctgcgg tccggccgtc gcaggccgcc
     7861 agcaagcggg cctcgccggc cgcgagcgcc gcactgccgc cgcccggcag ccgcaggcgc
     7921 tcgccctgga cttccaggaa cggctggcgc cgcgggatga gccatggcag caggcgctcg
     7981 tcacgggcca tgtcggcggc cagcgtcgcg atcggccagt cctcgaagcg gacgctgcgc
     8041 cggcgcacca gcccgctgcc tgcgtcgtag cgcgcctcgc gcggatcggc gacgaggcgg
     8101 gcccaggtca tggggccgaa gaacccgatg gtgtcgttct tcagcgcgta gcgttgcagg
     8161 tagccggcca ccagggcctc gcgctggcga cgcttcgaac cggacatggg cgcgccggcc
     8221 gcgagcgggt cgagcgcggt cgtcacggcg gcgtggttct gccaggtgac ggcttcgcgg
     8281 aacagcgggt cggcggccag ccgcgccgcg acggcggtga aggcatcgtt ggcggcggcc
     8341 aggctcacgg cccacgcggc ctgcagctcg tcgcagcggc gggcggccag cgccaggcgt
     8401 tcgtgcaacg gcgcgcccag cgatgcgggc agtgcgccgg cgacgtctcc gcgccgcagc
     8461 gccttgatct cgcgttgcca ctgccgcacc tggccttcgg ccgcctcgtc gccgcggtgc
     8521 gcgtcgatgc gcgcgccgat gcaggcgacg gcctcgcgca cgatcgcgtc gcgcatcgcg
     8581 cgggcctcgt cgagccggtc cgcgagctgg ctgcaggact cgtcgaccag cggccgcagc
     8641 atgtccgcgg gaaagccggc gctgcgcagc atgcagtcgc gccacagcgc ccagcccgcg
     8701 gcgctcggcg cggtcaggtg ggccggccag cgcgggcggg catcagcgac gggcgggtgc
     8761 tcggtggcgt cgtccaggaa gtgcaatcaa ggtctccggc aaggggcggc ggggcggccg
     8821 tcagcgcgcg agaagggcat cgacgtggcc ggccagcgcg cgcggcgttc gcagctgcac
     8881 gagctcgcgc agggccagcc gcacgcccag ggcctcgtgc aggcgcgtcg acaggcgcac
     8941 gagcaacatc gagtggaagc cgcggtcgaa gaggtcgtcg tcggcgtccg gtgcgccggc
     9001 gggcgcgaga ccggcgg
//
LOCUS       DATCRO010000758         8583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_188903, whole genome shotgun sequence.
ACCESSION   DATCRO010000758 DATCRO010000000
VERSION     DATCRO010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188903"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VIN75_20540"
     CDS             complement(<1..678)
                     /locus_tag="VIN75_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8976615.1"
                     /translation="MSALLDDLRALVGAAHVLVDDTGDALVAYERDWRKRYRGRALCV
                     VLPGSADEVAAVVRRCAREDGVSIVAQGGNTGLVGGGVPDDSRRQVVLGMRRLRAVRQ
                     VDVDNQTITVEAGLALQEVQAAAADAGLLFPLSLASEGSCTIGGNLATNAGGTQVLRF
                     GNARELCLGLEVVTAQGELWSSLHGLRKDNTGYDLRDLFVGSEGTLGLITAATLRLFP
                     RPRAGLTA"
     gene            complement(680..1105)
                     /locus_tag="VIN75_20545"
     CDS             complement(680..1105)
                     /locus_tag="VIN75_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY8976616.1"
                     /translation="MAEPPPDSPATPDRVTATTRIVAVASALGLIVLALAWELALARI
                     GHGSLAFKALPLVFALPGLLRHRMYTYRWVSLLVWLYVAEAVVRIGDRWPSNACAWAE
                     LALSIALFTACATQVRWRHAVARRLPAAEDPRANAGTEH"
     gene            1125..2360
                     /locus_tag="VIN75_20550"
     CDS             1125..2360
                     /locus_tag="VIN75_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY8976617.1"
                     /translation="MTVSKTLNTPMGSLLAGPMQTLRNWPWFETLRTLRARFRDDRLG
                     LTASSLTFTTLISLVPLLTVMLAVFTAFPMFSNFQAGLEKYFLQTLVPPNIAKPVLGA
                     LTMFATKASRLGAFGLIALGFTAMALILTIDRTLNAIWRVRKPRPIAQRVLIYWAAVT
                     LGPLILGASLTLMSYAISASSGMVAQMSGGVSFSLSLLEFVALATAMSALYHYVPHAP
                     VAWRHAVAGGLFVAVGFEVARRSLGWYLTHMTSYSSIYGAFATVPIFLLWIYLGWVIV
                     LLGAVIAAYAPSLKLNLVQHGRVPGQRFALALDIVRRLQRSREAGGHGMNGIDLADSL
                     RMDPLQIESVLEALMTLDWVGRLDEENQPRYVLLCDPAHTQLAPLVDVLLLAPIEALA
                     PARSKLGVDGLRLSEVLPA"
     gene            2388..2978
                     /locus_tag="VIN75_20555"
     CDS             2388..2978
                     /locus_tag="VIN75_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976618.1"
                     /translation="MATATASRARRRWLVAGVLLVAVVTAAWLVLHERWTFHLVDMRF
                     VDHREKVAGGGAALNVTGYVLVRVESGVDIFRQTHDDNAYPEVKAVLCDSGRRVGAWR
                     DPLPLERDAAGKRFVYAVLLPARGRDGELAQSAGAVCLRFMSAGQGLPAWGAAGSLTV
                     TLPPAMREQMLAYARRDGTVDVSLDPICAPLLCQPD"
     gene            complement(2980..3558)
                     /locus_tag="VIN75_20560"
     CDS             complement(2980..3558)
                     /locus_tag="VIN75_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY8976619.1"
                     /translation="MTRNPAVEPPKVGITLAELRQLRALSLDELSRLAGVSKSMLSQI
                     ERNQANPTVAVVWRLANALGVPLGQLLDGERPALPAITTVAGHATPSLRSPDGKCELR
                     ILGPIELAGQFEWYELTVQPGGRLESQAHEPGSREHLTVLGDGPLEVRSGTDVTTLQA
                     NETARYVVDVPHAISNPGERVVRALMVVVHAG"
     gene            3689..4900
                     /gene="kbl"
                     /locus_tag="VIN75_20565"
     CDS             3689..4900
                     /gene="kbl"
                     /locus_tag="VIN75_20565"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY8976620.1"
                     /translation="MADVAAFNQHIQSELDGIRAAGLFKSERIIATPQGALVKLADGR
                     ELINLCANNYLGLSSHPKVIEAAHEGLRTHGYGMSSVRFICGTQDIHKELEQRLARFL
                     GTEDTILYAAAFDANGGLFEPLLGEDDAVISDALNHASIIDGIRLCKAKRFRYAHNDL
                     ADLEVQLQAADAAGARFKLVFTDGAFSMDGTIAQLDKIRALCDRYGALLGIDECHATG
                     FLGARGRGTHEHRGVFGKIDIITGTLGKALGGASGGFTTGRKEVIELLRQRSRPYLFS
                     NTVMPAIVAGSLAVLDLLESSTELRDKLADNTAWFRQAITAAGFEIKPGEHPIVPIMI
                     GDAVKAQQLSQRILELGLYAVGFFFPVVPKGQARIRVQMSAVHDRHHLEKAVEAFTKA
                     GKELGLIGAAQ"
     gene            4897..5859
                     /locus_tag="VIN75_20570"
     CDS             4897..5859
                     /locus_tag="VIN75_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8976621.1"
                     /translation="MSGARILIIGANGQIGSELAGALSQRAGVEAVITSDVAPTGRTP
                     GLVHEQLDVTDAAALTAVVERHHVTQVYLLAAALSANGEKHPQWAWNLNMVGLLNVLE
                     LARTHKLDKVFWPSSIAAFGPSTPADGTPQDTVMDPTTIYGISKLAGERWCAWYHRNH
                     GVDVRSLRYPGLISWKTPPGGGTTDYAVEIFHAALRDGRYTCFLKEDQALPMMLMDDA
                     LRATIELMEAPADSIRQRGSYNLSAISFTPRQIADAIARRIPGFTMDCVPDFRQAIAA
                     NWPRSIDDSEAARDWGWKARFGLDEMVDEMLTQLAPIVKAKATA"
     gene            5955..6680
                     /locus_tag="VIN75_20575"
     CDS             5955..6680
                     /locus_tag="VIN75_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976622.1"
                     /translation="MTPREPFDHLRHAALDLLESGNSTASVSQLLAVPPDVIVRWRDE
                     PVPPRLDPAARLLALGAAGHAPHFSTTLVVRRCAPGDMWRHALQDYVVCGLLLVMVMM
                     AIRGLGHGGAFGSIWIDVTPLFGLGVLWLRRNQVLFKLDDRGIVVPRLFGSERLSYAD
                     LADWWLVMHVQGKDTDEEVEGRKLTLHSRRKGAPPIELFVADYVHIDQAVIERLDLVK
                     TANQGVRPLTPVRSIPKTPAWGG"
     gene            complement(6730..7053)
                     /locus_tag="VIN75_20580"
     CDS             complement(6730..7053)
                     /locus_tag="VIN75_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY8976623.1"
                     /translation="MANAVTNIESLHSFAEFYPFYLNEHSNATCRRLHFIGSTLALIC
                     LWSLIWTHQPLFFVASVAFGYGFAWIGHFVFEKNKPASFKRPLWSFMGDWRMYFDIWT
                     GKVPV"
     gene            complement(7081..7881)
                     /locus_tag="VIN75_20585"
     CDS             complement(7081..7881)
                     /locus_tag="VIN75_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976624.1"
                     /translation="MKFEVQGRAAYAYTGGKAFAVDGPCVVFIHGALNDHSVWTLLAR
                     WFAHHGHRVLAVDQPGHGRSEGPPLASVEALADWVLAMLDAAGVDQAHLVGHSMGSLI
                     ALEAAARAPARASSLVMVGTAYPMKVSDALLSTARDDPARAIAMVNAFSHSSLASKPS
                     YPGPGAWLRGGSSALMHRIQGDADGNLFEHDFRVCDAYAGGTEAARRVTCPAHLIVGT
                     RDVMTPAKATRELAGMLNAAVSRIVSGHALMQEAPDAVLNAVRQAIKA"
     gene            complement(7878..>8583)
                     /locus_tag="VIN75_20590"
     CDS             complement(7878..>8583)
                     /locus_tag="VIN75_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY8976625.1"
                     /translation="GADLVFHSATKFLSGHGTVVGGVLVDSGRFDWERSGRFPELTTP
                     YAGFHDMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGLETLPLRMERHVA
                     NARKVARFLASHPLVAQAGYPDLESHPDHALAQALLPRGCGSVFSFDLRGSRAQGVAF
                     IEALQLFSHLANVGDCRSLVIHPASTTHFRMDDAALAAAGIGPGTIRLSIGLEDADDL
                     IADLKAALKAAERAAS"
BASE COUNT         1265 a         3028 c         3028 g         1262 t
ORIGIN      
        1 ggccgtgagg ccggcgcgcg gccgcgggaa cagccgcagc gtggcggccg tgatcaggcc
       61 cagcgtgccc tcgctgccga cgaacaggtc gcgcaggtcg tagccggtgt tgtccttgcg
      121 cagaccgtgc aggctgctcc agagctcgcc ctgcgcggtc acgacctcga gccccaggca
      181 cagctcgcgc gcgttgccga agcgcagcac ctgcgtgccg ccggcgttgg tggcgaggtt
      241 gccgccgatg gtgcagctgc cctcggacgc caggctcagc gggaacagca ggcccgcgtc
      301 cgcggcggcc gcctgcacct cctgcagcgc gaggccggcc tcgacggtga tcgtctggtt
      361 gtcgacgtcg acctgccgca cggcgcgcag gcgccgcatg ccgagcacga cctggcgccg
      421 cgagtcgtcg ggcaccccgc cgcccaccag gcccgtgttg ccgccttgcg cgacgatgct
      481 gacgccgtcc tcgcgcgcgc agcggcgcac gacggccgcg acttcgtcgg ccgacccggg
      541 cagcacgacg cacagcgcgc ggccgcggta gcgcttgcgc cagtcgcgct cgtacgcgac
      601 gagcgcgtca ccggtgtcgt ccaccagcac gtgcgccgcg ccgacgaggg cgcgcaggtc
      661 gtcgagcagg gcgctcatct caatgctcgg tgccggcatt ggcgcgcggg tcctcggccg
      721 ccggcaggcg cctggccacc gcgtggcgcc agcgcacctg cgtcgcgcag gccgtgaaca
      781 gcgcgatgga cagcgcgagc tcggcccacg cgcaggcgtt cgagggccag cggtcgccga
      841 tgcgcacgac ggcctcggcc acgtagagcc acacgagcag gctgacccag cggtacgtgt
      901 acatgcgatg gcgcagcaac ccgggcaacg cgaacaccag cggcagcgcc ttgaaggcca
      961 gcgagccgtg gccgatgcgc gccagcgcca gctcccacgc gagcgccagc acgatcagcc
     1021 cgagggccga ggcgaccgcg acgatgcgcg tcgtggcggt gacgcggtcg ggcgtcgcgg
     1081 gggagtcggg cgggggctcg gccatgtttg gcatgatagt cgccatgacg gtttccaaga
     1141 cgctgaacac gccgatgggc tcgctgctcg ccggccccat gcagaccctg cgcaactggc
     1201 cctggttcga gacgctgcgc acgctgcgcg cgcgtttccg tgacgaccgc ctgggcctga
     1261 ccgcgagcag cctcaccttc acgacgctga tctcgctggt gccgctgctg acggtgatgc
     1321 tggccgtctt cacggcgttc ccgatgttct cgaatttcca ggccgggctg gagaagtact
     1381 tccttcagac gctggtgccg cccaacatcg ccaagccggt gctgggcgcg ctgacgatgt
     1441 tcgcgacgaa ggccagccgg ctcggcgcgt tcggcctgat cgcgctggga ttcacggcga
     1501 tggcgctgat cctcacgatc gaccgcacgc tcaacgcgat ctggcgcgtg cgcaagccgc
     1561 gcccgatcgc gcagcgcgtg ctgatctact gggccgccgt cacgctgggg ccgctgatcc
     1621 tgggcgcgag cctgacgctc atgtcgtacg cgatctcggc ctccagcggc atggtggcgc
     1681 agatgtccgg cggcgtgagc ttctcgctgt cgctgctcga attcgtggcg ctggccaccg
     1741 ccatgagcgc gctgtaccac tacgtgccgc acgcgccggt ggcctggcgc cacgccgtgg
     1801 ccgggggcct gttcgtggcg gtgggcttcg aggtggcgcg gcgatcgctg ggctggtacc
     1861 tgacgcacat gacgtcgtac tccagcatct acggcgcgtt cgccacggta cccatcttcc
     1921 tgctgtggat ctacctgggc tgggtgatcg tgctgctggg cgcggtgatc gccgcctacg
     1981 cgcccagcct caagctcaac ctcgtgcagc acggccgggt gccgggacag cgcttcgcgc
     2041 tggcgctgga catcgtgcgt cgcctgcagc gcagccgcga ggccggcggg cacggcatga
     2101 acggcatcga cctggccgac agcctgcgca tggatccgct gcagatcgag tcggtgctcg
     2161 aggcgctgat gacgctggac tgggtcggcc gcctcgacga ggagaatcag ccgcgctacg
     2221 tgctgctgtg cgacccggcg cacacgcagc tggcgccgct ggtcgacgtg ctgctgctgg
     2281 cgcccatcga ggcgctcgcg ccggcgcgca gcaagctggg cgtggacggc ctgcggctgt
     2341 ccgaggtcct gcctgcctga gcgcctcgcg cgtccgaccc gaccatcatg gccacggcaa
     2401 ccgcatcgag agcccggcgt cgctggctcg tggccggcgt gctgctcgtc gccgtcgtga
     2461 cggccgcctg gctggtgctg cacgagcgct ggacgttcca cctggtcgac atgcgcttcg
     2521 tcgaccaccg cgagaaggtg gccggcggcg gcgcagcgct gaacgtcacg gggtacgtgc
     2581 tggtgcgcgt cgagagcggc gtcgacatct tccgccagac ccatgacgac aacgcctacc
     2641 ccgaggtcaa ggcggtgctg tgcgactccg gccggcgcgt cggcgcgtgg cgtgacccgc
     2701 tgccgctcga acgcgacgcc gccggcaagc gcttcgtcta tgccgtgctg ctgcccgcgc
     2761 gcgggcgcga cggcgagctc gcgcagtcgg cgggcgccgt ctgcctgcgc ttcatgtcgg
     2821 ccggccaggg cttgcccgcc tggggcgccg cgggctcgct gaccgtgacg ctgccgccgg
     2881 cgatgcgcga gcagatgctg gcgtatgcgc gccgggacgg gacggtggac gtctcgttgg
     2941 acccgatctg cgcgcccctg ctttgccagc cggattgact tagccggcgt gcaccaccac
     3001 catcagcgcg cgcacgacgc gctcgccggg gttggagatg gcgtgcggca cgtcgaccac
     3061 gtagcgcgcg gtctcgttgg cctgcagcgt ggtcacgtcg gtgcccgagc gcacctccag
     3121 cgggccgtcg cccagcacgg tcaggtgctc gcgcgagccc ggctcgtggg cctgcgattc
     3181 gagccggccg ccgggctgca ccgtcagctc gtaccactcg aactggccgg cgagctcgat
     3241 cgggcccagg atgcgcagct cgcacttgcc gtccgggctg cgcagcgacg gcgtcgcgtg
     3301 gccggcgacg gtggtgatgg cgggcagggc ggggcgctcg ccgtccagca gctggccgag
     3361 cggcaccccc agtgcgttgg ccaggcgcca gacgaccgcg acggtcgggt tggcctggtt
     3421 gcgctcgatc tgcgacagca tcgacttgga cacccccgcc agccgtgaca gctcgtccag
     3481 cgacagtgcg cgcaattggc gcagttccgc cagggtgatg cccaccttcg gtggctcgac
     3541 agcagggttg cgggtcatga tggttttcgt cctggccgat gccccgccgc ttgacggatc
     3601 ggtcgatgtt cacgatactg cattcatgtt cgatatatcg aacaacgaat ttcgcattga
     3661 acatgattgt gcctgaagga caatgaacat ggccgacgtc gccgcattca accaacacat
     3721 ccagtccgaa ctggacggca tccgcgccgc ggggctgttc aagtccgagc gcatcatcgc
     3781 cacgccgcag ggcgcgctgg tcaagctggc cgacggccgc gagctgatca acctgtgcgc
     3841 caacaactac ctggggctat cctcgcaccc gaaggtgatc gaggcggcgc acgaaggcct
     3901 gcgcacgcat ggctacggca tgagctcggt gcgcttcatc tgcggcacgc aggacatcca
     3961 caaggagctc gaacagcgcc tcgcgcggtt cctgggcacc gaggacacga ttctttacgc
     4021 cgccgccttc gacgccaacg gcggcctgtt cgagccgctc ctgggcgagg acgacgccgt
     4081 catctcggac gcgctgaacc acgcctccat catcgacggc atccgcctgt gcaaggccaa
     4141 gcgcttccgc tacgcgcaca acgacctggc cgacctcgag gtgcagctcc aggccgccga
     4201 cgccgccggc gcgcgcttca agctcgtctt caccgacggc gcattctcga tggacggcac
     4261 gatcgcgcag ctggacaaga tccgcgcgct gtgcgaccgg tacggcgcgt tgctgggcat
     4321 cgacgaatgc cacgccaccg gcttcctggg cgcgcgcggc cgcggcacgc acgagcaccg
     4381 cggcgtgttc ggcaagatcg acatcatcac cggcacgctg ggcaaggcgc tgggcggcgc
     4441 gtcgggcggc ttcaccaccg gcaggaagga agtgatcgag ctgctgcgcc agcgctcgcg
     4501 tccctacctg ttctccaaca ccgtgatgcc ggcgatcgtc gcgggctcgc tggccgtgct
     4561 cgacctgctg gaatccagca cggagctgcg cgacaagctg gccgacaaca ccgcgtggtt
     4621 ccgccaggcc atcacggccg ccggcttcga gatcaagccg ggcgagcacc cgatcgtgcc
     4681 gatcatgatc ggcgacgccg tcaaggcgca gcagctgtcg cagcgcatcc tggagctggg
     4741 cctgtacgcg gtcggcttct tcttccccgt cgtgcccaag gggcaggcgc gcatccgggt
     4801 gcagatgtcg gcggtgcatg accggcatca cctggagaag gccgtcgagg cgttcaccaa
     4861 ggccggcaag gaactgggcc tgatcggagc cgcgcaatga gcggcgcccg catcctcatc
     4921 atcggcgcca acggccagat cggctccgag ctggccggtg cgctgagcca gcgcgcgggc
     4981 gtcgaggccg tcatcaccag cgacgtcgcg cccaccggcc gcacgccggg cctggtgcac
     5041 gagcagctgg acgtcacgga cgccgccgcg ctgacggccg tcgtcgagcg tcaccacgtc
     5101 acgcaggtct acctgctggc ggcggcgctg tcggccaacg gcgagaagca cccgcagtgg
     5161 gcctggaacc tgaacatggt gggcctgctc aacgtgctcg aactggcccg cacgcacaag
     5221 ctggacaagg tgttctggcc cagctcgatc gcggccttcg gcccgtcgac gcccgccgac
     5281 ggcacgccgc aggacacggt catggacccg accacgatct acggcatcag caagctggcc
     5341 ggcgagcgct ggtgcgcctg gtaccacagg aaccacggcg tcgacgtgcg cagcctgcgc
     5401 tacccgggcc tgatcagctg gaagacgccc ccgggcggcg gcaccaccga ctacgccgtg
     5461 gagatcttcc acgccgcgct gcgcgacggc cgctacacct gcttcctcaa ggaggaccag
     5521 gcgctgccga tgatgctgat ggacgacgcg ctgcgcgcca ccatcgagct gatggaggcg
     5581 ccggccgact cgatccgcca gcggggcagc tacaacctgt cggccatcag cttcacgccg
     5641 cgccagatcg ccgacgccat cgccaggcga attccgggct tcacgatgga ctgcgtcccc
     5701 gacttccgcc aggcgatcgc cgccaactgg ccgcgctcga tcgacgacag cgaggcggcc
     5761 agggactggg gctggaaggc caggttcggc ctcgacgaga tggtcgacga gatgctcacg
     5821 caactcgcgc cgatcgtgaa ggcgaaggcg acggcctgat ccggccgcca gtgttggccc
     5881 cgcgcgcgtg cggtgcgtca cgcgcgcgac gtgcgcctgc gcgcggcgcc gatgccgcgg
     5941 ccgataatgc gggcatgacg ccccgagagc cgttcgacca cctgcgccac gccgcgctcg
     6001 acctgctgga atcgggcaac agcacggcct cggtgtcgca gctgctggcc gtgccgccgg
     6061 acgtgatcgt gcgctggcgc gacgaaccgg tgccgccgcg cctggacccg gccgcccgcc
     6121 tgctggcgct cggcgctgcc ggccatgcgc cgcacttctc cacgacgctg gtggtcaggc
     6181 gctgcgcgcc gggcgacatg tggcgtcacg cgctgcagga ctacgtggtg tgcggcctgc
     6241 tcctcgtgat ggtcatgatg gcgatccgtg ggctgggtca cggcggtgcg ttcggctcga
     6301 tctggatcga cgtgacgccg ctgttcggcc tcggcgtgct ctggctgcgg cgcaaccagg
     6361 tgctgttcaa gctcgacgac cgcggcatcg tggtgccgcg cctgttcggc agcgaacgcc
     6421 tgtcgtacgc ggacctcgcc gactggtggc tggtgatgca cgtccagggc aaggacaccg
     6481 acgaggaagt cgaaggccgc aagctgacgc tgcattcgcg gcgcaagggt gcgccgccca
     6541 tcgaactgtt cgtcgccgac tatgtccaca tcgaccaggc cgtgatcgag cggctcgatc
     6601 tcgtgaagac ggcgaaccag ggcgtccgtc cgctgacgcc ggtgcgctcg atccccaaga
     6661 caccggcgtg gggcgggtag gccgaaggcg tcatccgccc ttcgcgcgct gccgcgcgac
     6721 gccgcccgct cagaccggca ccttccccgt ccagatgtcg aagtacatcc gccagtcccc
     6781 catgaagctc cacagcggcc gcttgaacga cgccggcttg ttcttctcga agacgaagtg
     6841 cccgatccag gcgaagccgt agccgaacgc gacgctggcg acgaagaaca gcggctggtg
     6901 ggtccagatc agcgaccaca ggcagatcag cgccagcgtc gagccgatga agtgcaggcg
     6961 gcggcaggtg gcgttgctgt gctcgttcag gtagaacgga tagaactcgg cgaagctgtg
     7021 cagcgattcg atgttggtga ctgcgttggc catggtcgtt ccctcgttcg ttatgcggcc
     7081 tcaggccttg atggcttgac gcaccgcatt caacaccgca tcgggggcct cctgcatcag
     7141 tgcatgccct gaaacgatgc gtgagaccgc agcattcaac atgcccgcga gctccctggt
     7201 cgccttggcg ggtgtcatca cgtcgcgcgt tcccacgatc aggtgggccg gacacgtgac
     7261 gcgccgggcc gcctcggtgc ctccagcgta cgcgtcgcag acgcggaagt catgctcgaa
     7321 caggttgcca tcggcatcgc cttgtatccg gtgcatcaag gcgctgctgc caccgcgcag
     7381 ccaggccccc gggccggggt acgacggctt gctggccagg ctggaatgcg agaaggcgtt
     7441 gaccatcgcg atcgcgcgcg ccgggtcgtc gcgggcggtg gagagcaggg catcgctgac
     7501 cttcatcgga tacgccgtcc cgaccatcac gaggctgctc gcccgcgcgg gggcgcgcgc
     7561 ggcggcctcc agcgcgatca gcgagcccat gctgtgcccg acgaggtggg cctggtcgac
     7621 gccggcggcg tccagcatcg cgagcaccca gtcggcaagc gcctcgacgc tggccagtgg
     7681 cggcccctcg gagcgcccgt gccccggctg gtcgaccgcc agcacgcggt ggccgtggtg
     7741 cgcgaaccag cgcgccagca gcgtccacac ggagtggtcg ttgagcgcgc cgtggatgaa
     7801 gacgacgcac ggcccgtcga cggcgaacgc cttgccgccg gtgtacgcgt aggccgcgcg
     7861 gccctggacc tcgaacttca tgacgcggcc ctctcggcgg ccttgagcgc ggccttcagg
     7921 tcggcgatca ggtcgtcggc gtcctccagg ccgatcgaca ggcggatcgt gccgggcccg
     7981 atgccggcag cggcgagcgc ggcgtcgtcc atccggaagt gcgtcgttga cgccggatgg
     8041 atcaccagcg agcggcagtc gcccacgttg gccaggtgcg agaacagctg cagcgcctcg
     8101 atgaacgcga cgccctgcgc gcgcgagccg cgcaggtcga agctgaagac cgagccgcag
     8161 ccgcgcggca gcagggcctg cgcgagcgca tggtcggggt gcgactcgag gtcggggtag
     8221 ccggcctgcg ccaccagcgg atgcgaggcc aggaagcgcg ccaccttgcg cgcattggcc
     8281 acgtggcgct ccatgcgcag cggcagcgtc tcgagcccct gcaggatcag ccaggccgtg
     8341 tgcgggctca tgcaggcgcc gaagtcgcgc aggccttcgc ggcgggcgcg cagcaggaac
     8401 gcgccgacgg tgctctcctc ggtgaacacc atgtcgtgga agccggcgta gggcgtagtc
     8461 agctcgggga agcgtccgct gcgctcccag tcgaagcggc cggagtccac cagcacgccg
     8521 ccgacgacgg tgccgtgtcc ggacaggaac ttggtggcgg aatggaacac gaggtcggcg
     8581 ccc
//
LOCUS       DATCRO010000759         8342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_244894, whole genome shotgun sequence.
ACCESSION   DATCRO010000759 DATCRO010000000
VERSION     DATCRO010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244894"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VIN75_20595"
     CDS             complement(<1..185)
                     /locus_tag="VIN75_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY8976626.1"
                     /translation="MNAPEWLHPEVIGGTVLYAVIGVVVLWLALIIIDWITPYKLWEQ
                     IVEEKNLALAVMVGSMF"
     gene            complement(330..1949)
                     /locus_tag="VIN75_20600"
     CDS             complement(330..1949)
                     /locus_tag="VIN75_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY8976627.1"
                     /translation="MATAPQRAWRAACPNCGAPVDFLSPASASAVCSFCRSTLVRDGE
                     SLRKIGTSAEVFDDFTPLAIGASGSYQGTSFMLVGRLQFGTDAGPWNEWHALFDNGRS
                     GWLSEDNGRYVFAFDLPLAAGTPLPEQLQAGSPVTVDGRPWRVASVVRAKLIAAQGEL
                     VQPPPSDRAIVVSDLRNTADEVGTLESVDGRLTSWSIGRSVRLADLRMSGLRETGIGG
                     AGEKTLKAQSPPCPNCGAPLRITLATTQSVVCDSCKSVVDLSQGIGPDMAHYAQLNRM
                     EPGIPLGRTGTLPIPSGAAPLPWQVVGYMERMELMSSGSDEAEAWREYLLYNRQEGFA
                     FLVDTNEGWSVVRVLTGVPESRGDQATWYGTRYQRRWTYTSETTYVLGEFYWKVEANQ
                     RTQHEDYEGSASGHRAFLSKESTASETTWSAGESIPAPMVAQVFGLDAAAGAQLQRGG
                     PTGSGPPSLGSIVGAGGLISWPVLIVLLIVVLVLLAWCSQDSCRDVKRAYGAYSAEYQ
                     QCKARSAAGTGAYYGTGGAWGGYSSGGGGHK"
     gene            complement(2049..3083)
                     /locus_tag="VIN75_20605"
     CDS             complement(2049..3083)
                     /locus_tag="VIN75_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8976628.1"
                     /translation="MSLMGFIKKQFIDVIEWQEPGDGILAWRFPMQDNEIQNGARLTV
                     RESQAALFVNEGRAADAFGPGLYTLNTHTLPLLTNLMNWDKLFASPFKSDVYFFSTRL
                     QVDQRWGTPQPVTLRDKDFGAIRIRAFGNYSWRVADPKLFQQQVSGTRGTYTVDEIDG
                     QLRGLMLQHLSDAVAQSGIPFLDLAANQVEFATQIKAAVAPAFANLGLALDVVTVQNV
                     SLPDELQKILDQKIGMGMVGQNMGAFMQYQTAQSIPDMAKAAGEGGGGVAGAGLGLGA
                     GVALGQVLAGNLAGGLGTGIATAAAASGEHPPVQDVMALIEKLGDLKAKGILTEEEFA
                     AKKTELLKKL"
     gene            complement(3309..3515)
                     /locus_tag="VIN75_20610"
     CDS             complement(3309..3515)
                     /locus_tag="VIN75_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976629.1"
                     /translation="MSNTNKSAAPITTLLLAAVVALEVTGMQALPHTDGRTLTATSVD
                     GDAGYMLVETPGSPPAAVVAQAGY"
     gene            complement(3763..5019)
                     /locus_tag="VIN75_20615"
     CDS             complement(3763..5019)
                     /locus_tag="VIN75_20615"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY8976630.1"
                     /translation="MTILVLGSGVIGTTIAYYLAKAGHDVTVVDRQGAPALETSFANA
                     GEVSPGYASPWAGPGVPVKAIKWLLMNHSPLIIKPMLEASMWRWGFAMLRNCTEARYR
                     INKSRMVRVAEYSRDCLKQLRADTGIAYDERTQGTLQLFRTQKQLDGVHKDTEILDQY
                     GVPYQVLDTAGYLQYEPALAGVRHKFVGALRLPGDETGDCFLFTQRLAKMAEALGAKF
                     RFNVEITGIASDGKRVTGVDTTAGRLTADHVVLALGSFSPKWLEPLGIAIPVYPVKGF
                     SITVPITDAAAAPESTIMDETHKVAVTRLGDRIRVGGMAQLSGYDLALDDRRRRTLEF
                     VLTDLFPAAGDLARATFWTGLRPMTPDGTPVMGGTKLENLTLATGHGTLGWTMAAGTG
                     RAIADLLGGRRPEIDFDGLTAARYGA"
     gene            5143..7872
                     /locus_tag="VIN75_20620"
     CDS             5143..7872
                     /locus_tag="VIN75_20620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8976631.1"
                     /translation="MQKNHRLLALSAAVAAAAAAFSAQAAGLPAQVREVEGVHEYRLP
                     NGLQVLLIPDASKPTVTVNVTYRVGSRLEGYGETGMAHLLEHLMFKSTRNIAQVSTEL
                     SRRGMQFNGTTSDDRTNYFETFPADPAQLSWALRTEAERMVHANVIKKDLDSEMTVVR
                     NEMEMNENNPFRILMEKTNAAAFEWHAYGKDTIGARSDVENVNIPHLQAFYRKYYQPD
                     NATLIVAGKFDPQKTLAQIAQDFGAIPRPTRVIEPTYTVEPVQDGEREVTLRRVGGEK
                     DLFVTYHTPSIASPDMPAFSVIGITLGDTPNGRLHKRLVEAGKATQVFAWPARHTDPG
                     TFNVGAVLKKDDDLDAAQKIFLDTIEGLKSEPITDDELKRAKLQIDKGFDQLLANPQA
                     LCVTLSDYIAGGDWRLLFLQRDRVDALTVDQVSKVAAAWIKSSNRTVGRFVPTDAPDR
                     APYAVRTDPEAQLADFKPRAAVAAGETFDATPAGIDKRSTVVTLPNGMKLALLPKKSK
                     GGTVEMRLDLHFGSLESLRGQRAAAEAAGIILGTGTETKTRAQLHDAFSALKTDWELT
                     GGTTWAHASLTTRREALIPALELLAEAMRKPSFTASEYEQGVRQQVQDLEQAAEEPGS
                     VAGNELARAQQHFAADDPRYTPSFAEWIAGTQATRLDDAIAFYRKFWGAEHGDMAIVG
                     DFDADQVKAAVTRLFGDWKSSQPFVRIQTPVTDVAGAHLSLPLKDKANAVLLASLPLT
                     LSDTDPDYPALTLATHVLGGGEFGSRLNARIRQKDGLSYNVGAGLDASPFEQRGSIGM
                     QAIFAPQNRARVQSDFDEELARFVKDGITPAELAEAKQAILAERATARTSDSVVASNW
                     MFKLDQGRTWAWSAEQDARLSALTVDQVNAAIRKWIVPAKVNWAVAGTFDEKK"
     gene            7872..8108
                     /locus_tag="VIN75_20625"
     CDS             7872..8108
                     /locus_tag="VIN75_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEY8976632.1"
                     /translation="MAADDELAPLPPAEPGRYRHYKGGEYELVGVARHSETLEPLVIY
                     RPLYNASGLWARPHAMFFGEIEVDGAAVRRFVKI"
BASE COUNT         1275 a         2935 c         2910 g         1222 t
ORIGIN      
        1 atgaacatcg agccgaccat gacggccagc gccaggttct tctcctcgac gatctgctcc
       61 cacagcttgt agggcgtgat ccagtcgatg atgatgaggg ccagccacag cacgacgacg
      121 ccgatcacgg cgtacaggac cgtgccgccg atcacctcgg ggtgaagcca ttcgggtgcg
      181 ttcatgtcgt tctccttgct tcgtgtgtga cgtcattcaa ggcgtgctga cgaagtcagc
      241 cgcccattca agctctcggt aagcgaggat cgcatccgag ccgatccgag cgccccctcg
      301 gggggcagcg acgggaggag cgtgggggct catttgtgcc cgccgccgcc ggacgaatag
      361 ccgccccagg cgccgccggt gccgtagtag gcgcccgttc ccgcggccga gcgcgccttg
      421 cattgctggt attccgcgct gtacgcgccg tacgcgcgct tgacgtcgcg gcacgagtcc
      481 tggctgcacc aggccagcag cacgagcacg acgatcagca gcacgatcag caccggccag
      541 gagatcagcc cgcccgcgcc gacgatgctg cccagcgacg gcgggccgct gcccgtcggg
      601 ccgccgcgct gcaactgcgc gcccgcggcc gcgtcgagcc cgaagacctg cgccaccatg
      661 ggcgcgggga tggactcgcc ggccgaccac gtcgtctcgc tggccgtgct ttccttgctc
      721 aggaacgcgc ggtggccgct ggccgagcct tcgtagtcct cgtgctgcgt gcgctggttc
      781 gcctcgacct tccagtagaa ctcgccgagc acgtaggtcg tctcggaggt gtacgtccag
      841 cgccgttgat accgggtgcc gtaccacgtg gcctggtcgc cgcgcgactc gggcacgccc
      901 gtgagcacgc gcacgacgct ccagccctcg ttggtgtcga cgaggaatgc gaagccttcc
      961 tgccggttgt acagcaggta ttcgcgccag gcctcggcct cgtcggagcc ggacgacatc
     1021 agctccatgc gctccatgta gccgacgacc tgccacggca gcggcgcggc gccgctgggg
     1081 atcggcagcg tgccggtgcg gcccagcggg atgcccggct ccatgcggtt cagctgcgcg
     1141 tagtgcgcca tgtccgggcc gatgccctgg gacaggtcga ccaccgactt gcagctgtcg
     1201 cacaccaccg actgcgtggt ggccagcgtg atcctcagcg gcgcgccgca attggggcag
     1261 ggcggcgact gcgccttgag cgtcttctcg ccggcgccgc cgatgccggt ctcgcgcagg
     1321 cccgacatcc tgaggtcggc caggcgcacc gagcggccga tcgaccagct cgtgagccgg
     1381 ccgtcgaccg actccagcgt gccgacctcg tcggccgtgt tgcgcaggtc cgagacgacg
     1441 atcgcgcgat cggacggcgg cggctgcacc agctcgccct gcgcggcgat cagcttggcg
     1501 cgcacgacgg aggcgacccg ccacggccgg ccgtcgacgg tgaccggcga gccggcctgc
     1561 aactgctcgg gcagcggcgt gcccgcggcg agcggcaggt cgaacgcgaa gacgtagcgg
     1621 ccgttgtcct ccgacagcca gccgctgcga ccgttgtcga acagcgcgtg ccattcgttc
     1681 cacgggccgg cgtcggtgcc gaactgcagc cggccgacca gcatgaagct ggtgccctgg
     1741 tacgagccgc tcgcgccgat cgccagcggc gtgaagtcgt cgaagacctc ggcgctggtg
     1801 ccgatcttgc gcagcgactc cccgtcgcgc accagcgtgc tgcggcagaa gctgcagacc
     1861 gcgctggccg acgcgggcga gaggaagtcg accggcgcgc cgcagttggg gcaggctgcg
     1921 cgccaggcgc gttgcggggc ggtggccatc aggcggccgt ccccgcgaac gcgcgcatga
     1981 gcgcgtcacg cgcgtgcgcg gtcacgcgcg acgcccgcag gatgcgccga cgatggcggg
     2041 ggcgggcctc acagcttctt gagcagctcg gtcttcttgg cggcgaactc ttcctcggtg
     2101 aggatgccct tggccttgag gtcgcccagc ttctcgatca gcgccatgac gtcctgcacc
     2161 ggcggatgct cgccgctggc ggccgccgcg gtggcgatgc ccgtgcccag gccgcccgcg
     2221 aggttgccgg ccagcacctg gcccagcgcg acgccggcgc ccaggcccag gcccgcgccg
     2281 gcgacgccgc caccgccttc gcccgcggcc ttggccatgt cggggatgga ctgcgccgtc
     2341 tggtactgca tgaaggcgcc catgttctgg ccgaccatgc ccatgccgat cttctggtcg
     2401 aggatcttct gcagctcgtc gggcagcgac acgttctgca ccgtgacgac atcgagcgcc
     2461 aggcccaggt tggcgaacgc cggcgcgacg gcggccttga tctgcgtggc gaactcgacc
     2521 tggttggcgg ccaggtcgag gaacgggatg ccgctctggg ccaccgcgtc ggacaggtgc
     2581 tgcagcatca gcccgcgcag ctggccgtcg atctcgtcga ccgtgtacgt gccgcgcgtg
     2641 cccgagacct gctgctggaa cagcttcggg tcggcgacgc gccaggagta gttgccgaac
     2701 gcgcggatac ggatcgcgcc gaagtccttg tcgcgcagcg tgacgggctg cggcgtgccc
     2761 cagcgctggt cgacctgcag gcgcgtgctg aagaagtaga catcgctctt gaacggggac
     2821 gcgaacagct tgtcccagtt catcaggttg gtcagcagcg gcagcgtgtg cgtgttcagc
     2881 gtgtacaggc cgggcccgaa cgcatcggcc gcgcggcctt cgttgacgaa cagcgccgcc
     2941 tgcgactcgc gcacggtcag gcgggcgccg ttctggatct cgttgtcctg catgggaaag
     3001 cgccacgcga ggatgccgtc gccaggctcc tgccattcga tgacgtcgat gaactgcttc
     3061 ttgatgaatc ccatcaaaga catggcggcc tccacttggc tccggggttg cattcagggc
     3121 ggatgataga cgcacggcgg gccgcttccc gtccgggtcg accgccggcg ccgccgggcc
     3181 gtcgaacgca cgttttccgt cgtccaacgg cgcctcgcgt cgccgggccg gcaagaggaa
     3241 agggccggcc cgcgcgatgc ggagcggccc ttcgtgcgca tgcccgcgga cgcgggcgcg
     3301 gcgggtgttc agtagccggc ttgcgccacg acggcggcgg ggggcgaccc cggggtctcg
     3361 accagcatgt agccggcatc gccgtcgacg ctggtcgcgg tcagcgtgcg gccgtccgtg
     3421 tgcggcagcg cctgcatgcc ggtcacctcg agggcgacga cggcggcgag cagcagcgtg
     3481 gtgatggggg cggcggactt gttcgtgttc gacatgacga ttccctccag ggtcggcgcg
     3541 cgagcgcgtg gcccgctgcg tccggtgcgc cgtgcctccg gcggaggctt gttgtggcca
     3601 gcgcgaggcg atcgtaggac tgcgaatccg gttggggcgc acttcgcgcg gactcgacag
     3661 ggaggccggc gtcgaataga tcacggttac aagatgtttc catgcgggcg acccccttcg
     3721 acggcctgac gagcggccga tagggtcctt ggaaacccgg gttcaggcgc cgtagcgcgc
     3781 ggccgtgagg ccgtcgaagt cgatctcggg ccggcggccg cccagcaggt cggcgatcgc
     3841 gcgccccgtg ccggcggcca tcgtccagcc cagcgtcccg tggccggtgg ccagcgtcag
     3901 gttctccagc ttggtgccgc ccatcaccgg cgtgccgtcg ggcgtcatcg ggcgcaggcc
     3961 ggtccagaac gtggcacgcg ccaggtcgcc ggcggcgggg aacaggtcgg tcagcacgaa
     4021 ttcgagcgtg cggcggcggc ggtcgtcgag ggccaggtcg taaccggaga gctgcgccat
     4081 cccgcccacc cggatgcggt cgcccaggcg cgtgaccgcc accttgtgcg tctcgtccat
     4141 gatggtggac tccggcgccg cggcggcgtc cgtgatcggc acggtgatcg agaagccctt
     4201 gaccggatag accgggatgg ctatccccag cggctcgagc catttcggcg agaagctgcc
     4261 gagcgccagc acgacgtggt cggccgtgag ccggccggcc gtcgtgtcga cgccggtgac
     4321 gcgcttgccg tcgctcgcga tgccggtgat ctccacgttg aagcggaact tcgccccgag
     4381 cgcctcggcc atcttcgcca gccgctgggt gaacaggaag cagtcgcccg tctcgtcgcc
     4441 gggcaggcgc agcgcgccga cgaacttgtg cctgacgccg gccagcgcgg gctcgtactg
     4501 caggtagccg gcggtgtcga gcacctggta gggcacgccg tactggtcga ggatctcggt
     4561 gtccttgtgc acgccatcga gctgcttttg cgtgcggaac agctgcagcg tgccctgggt
     4621 gcgctcgtcg taggcgatgc cggtgtcggc gcgcagttgc ttgagacagt cgcggctgta
     4681 ttccgcgacg cgcaccatgc gcgacttgtt gatgcggtag cgcgcctcgg tgcaattgcg
     4741 cagcatcgcg aaaccccagc gccacatcga ggcctcgagc atcggcttga tgatcagcgg
     4801 gctgtggttc atcagcagcc acttgatcgc cttgaccggc acgcccgggc cggcccacgg
     4861 cgatgcatag cccggagaga cttcgccggc gttggcgaag ctggtctcca gcgccggggc
     4921 gccctggcgg tcgacgaccg tgacgtcgtg gcccgccttg gccaggtagt aggcgatcgt
     4981 ggtgccgatg acgccgctgc cgaggacgag gatggtcatg gtggggcgcc cgctgtgctc
     5041 gtggtggggc cgactatcgc acgcggcgcc cgtccgacgg aaccatcggc ccggcgccag
     5101 ctcagagcga ctccattcac gagccgtgcc aagatgcgcc ccatgcaaaa aaaccatcgc
     5161 ttgctggccc tcagcgccgc cgtcgccgcc gccgcggccg ccttctccgc ccaggccgcc
     5221 ggccttcccg cgcaggtgcg cgaggtcgag ggcgtgcacg agtaccggct gcccaacggg
     5281 ctgcaggtgc tgctgatccc ggacgcctcc aagcccacgg tcaccgtcaa cgtgacctac
     5341 cgcgtcggca gccgcctgga gggctacggc gagacgggca tggcccacct gctcgagcac
     5401 ctgatgttca agagcaccag gaacatcgcg caggtcagca cggagctgtc caggcgcggc
     5461 atgcagttca acggcaccac ctccgacgac cgcaccaact acttcgagac cttcccggcc
     5521 gacccggcgc agctgtcgtg ggcgctcagg accgaggccg aacgcatggt gcacgccaac
     5581 gtcatcaaga aggacctcga ctccgagatg acggtggtgc gcaacgagat ggagatgaac
     5641 gagaacaacc cgttccgcat cctcatggaa aagaccaacg cggccgcctt cgagtggcac
     5701 gcgtacggca aggacaccat cggcgcgcgt tcggacgtcg agaacgtcaa catcccgcac
     5761 ctgcaggcct tctaccgcaa gtactaccag ccggacaacg ccacgctgat cgtcgccggc
     5821 aagttcgacc cgcagaagac gctggcgcag atcgcgcagg acttcggcgc gatccccagg
     5881 cccacccgcg tgatcgagcc cacgtacacg gtcgagcccg tgcaggacgg cgagcgcgag
     5941 gtgacgctgc gccgcgtcgg cggcgagaag gacctcttcg tcacgtacca cacgccgtcg
     6001 atcgccagcc ccgacatgcc ggccttcagc gtcatcggca tcacgctcgg cgacacgccc
     6061 aacggccgcc tgcacaagcg cctggtcgag gccggcaagg ccacgcaggt cttcgcctgg
     6121 ccggcgcgcc acaccgaccc gggcaccttc aacgtcggcg cggtgctcaa gaaggacgac
     6181 gacctggacg ccgcgcagaa gatcttcctg gacaccatcg agggcctgaa gtccgagccc
     6241 atcaccgacg acgagctcaa gcgcgcgaag ctgcagatcg acaaggggtt cgaccagctg
     6301 ctggccaatc cgcaggccct gtgcgtgacg ctgagcgact acatcgccgg cggcgactgg
     6361 cgcctgctgt tcctgcagcg tgaccgcgtc gacgccctga cggtcgacca ggtcagcaag
     6421 gtggcggccg cctggatcaa gtcgtccaac cgcacggtgg gccgcttcgt cccgaccgac
     6481 gcgcccgatc gcgcgccgta cgcggtgcgc accgatccgg aggcgcaact ggccgacttc
     6541 aagccgcgcg ccgccgtcgc cgccggcgag accttcgacg ccacgccggc cggcatcgac
     6601 aagcgcagca ccgtggtgac gctgcccaac ggcatgaagc tcgcgctgct gcccaagaag
     6661 agcaagggcg gcaccgtgga gatgcgcttg gacctgcact tcggctcgct ggaatcgttg
     6721 cgcggccagc gcgcggcggc cgaggccgcg ggcatcatcc tgggcacggg caccgagacg
     6781 aagacgcgcg cgcaactgca cgatgcgttc tccgcgctca agaccgactg ggaactgacc
     6841 ggcggcacga cctgggccca tgcgtcgctg accacgcggc gcgaggcgtt gatcccggcg
     6901 ctggagctgc tggccgaggc gatgcgcaag ccgtcgttca cggcgtccga gtacgagcag
     6961 ggcgtgcgcc agcaggtgca ggacctggaa caggccgccg aggagccggg cagcgtcgcc
     7021 ggcaacgagc tggcgcgcgc gcagcagcat ttcgccgccg acgacccgcg ctacacgccg
     7081 tccttcgccg aatggatcgc cgggacgcag gccaccaggc tcgacgacgc gatcgcgttc
     7141 taccggaagt tctggggcgc cgaacatggc gacatggcca tcgtcggcga cttcgacgcc
     7201 gaccaggtca aggccgcggt cacgcgcctg ttcggcgact ggaagtcctc gcagcctttc
     7261 gtgcgcatcc agaccccggt gaccgacgtc gccggcgcgc acctgtcgct gccgctcaag
     7321 gacaaggcca acgcggtgct gctggccagc ctgccgctga cgctgagcga caccgacccc
     7381 gactatccgg cgctcacgct ggccacccac gtgctgggcg gcggcgagtt cggctcgcgc
     7441 ctgaacgcgc gcatccgcca gaaggacggc ctgtcgtaca acgtcggcgc cggcctcgac
     7501 gcctcgccgt tcgagcagcg cggctcgatc ggcatgcagg ccatcttcgc gccgcagaac
     7561 cgcgcccgcg tgcagtccga cttcgacgag gagctggcgc gcttcgtcaa ggacggcatc
     7621 acgccggccg agctcgccga ggccaagcag gcgatcctcg cggaacgcgc caccgcgcgc
     7681 accagcgact cggtggtcgc cagcaactgg atgttcaagc tcgaccaggg ccgcacctgg
     7741 gcctggagcg ccgagcagga cgccaggctg tcggccttga cggtcgatca ggtcaacgcg
     7801 gcgatccgca agtggatcgt ccccgccaag gtcaactggg ccgtggccgg cacattcgac
     7861 gagaagaagt gatggccgcc gacgacgagc tcgcgcccct gccgcccgcc gagcctggcc
     7921 gttaccgcca ctacaagggc ggcgagtacg agctggtcgg cgtggcgcgc cacagcgaga
     7981 cgctcgagcc gctcgtgatc taccggccgc tctacaacgc gagtggcctg tgggcgcggc
     8041 cgcatgcgat gttcttcggc gaaatcgagg tcgacggcgc cgcggtgcgt cgcttcgtca
     8101 agatctgaat cagccggcgg ccgcctggcg gggcagggcc gtcgtttcac ggctgaaaat
     8161 cgacggtcgg tcgcgtcggc ggggccgcgc ctgcccggtg ccgatatgct tgcgcctttc
     8221 gaaccgtcca cgagagggtt cgatggcccg aacaaagaat ccgaggagct tgcctgatga
     8281 cgctgtcccg ccgcttcgct gcgccccgta cgaccacgat cgccgtggcg atcgccctgg
     8341 cc
//
LOCUS       DATCRO010000760         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_121112, whole genome shotgun sequence.
ACCESSION   DATCRO010000760 DATCRO010000000
VERSION     DATCRO010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121112"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VIN75_20630"
     CDS             <1..1696
                     /locus_tag="VIN75_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8976633.1"
                     /translation="QASGRSATFGELAPKAAQQPLPTQVALKKPADFKLIGQPARRMD
                     GAAKLDGSATFGLDVLPPGLLYASIAMCPTTGGRVASFDATAAQKLPGVRKVMALEPV
                     GATLIGTGATPGGVAVIADTPYHAMRAVKALAIEWDHGPAASLSSSEMIERLSQTLRT
                     RPGNARLDDGDVAGAFRSAAKTIEAEYRVPFLAHATMEPMNCTVQFKDGKATVWAPTQ
                     APGFTRGAAAKALGIDADKVELHVTYLGGGFGRRYSTDFVTQAATLARETGGAPVQLF
                     WSREEDMAHDFYRPAYVARCRAGFDAAGALVAWQTVTAGSSMGAPSLMDTATDGAWNT
                     AYAFPNARVAHVPVESAMPTGVWRSVAHSQNGFFVESFIDECAAAAGKDPVAFRAALL
                     AKDERHLRVLRRVAELSNWSQPPAPGPDGAKRARGLAIHRSFGSIVAQVAEVSVSPDR
                     QVRVHRVTCVVDCGVAVNPNLIRQQMEGAIVYGLSAALHGEITVEKGRVQQSNFHDYM
                     PLRMNECPAIEVEIAASGEAPGGVGEPGTPPIAPAVANAVFALTGQRLRSLPLRLA"
     gene            1726..2187
                     /locus_tag="VIN75_20635"
     CDS             1726..2187
                     /locus_tag="VIN75_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8976634.1"
                     /translation="MTTTLTVNGKAVTVNAEPDTPLLWVLRGEMQLVGTKFGCGKALC
                     GACTVHLDGEPVRSCVTPLEAVGSREVTTIEGLKGREADALRNAWTEVDVVQCGYCQS
                     GQLMSACALLKKTPSPSDDDISGAMSGNICRCGTYPRIRAAIHVAARKAAG"
     gene            2438..>2680
                     /locus_tag="VIN75_20640"
     CDS             2438..>2680
                     /locus_tag="VIN75_20640"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8976635.1"
                     /translation="MADTLRDRIFDGEIAPGSHLMEISLANELGVSRTPVRGAMARLA
                     DEGLLVYLPNKGFQVRRFNAKDVYDAFSVRATLEGMC"
BASE COUNT          439 a          955 c          909 g          377 t
ORIGIN      
        1 ccaggccagc ggcaggagcg ccactttcgg ggagctggcc cccaaggccg cgcagcagcc
       61 cctgccgacg caggtggcgc tgaagaagcc ggccgatttc aagctcatcg gccagcccgc
      121 gcggcgaatg gacggcgccg ccaagctcga cggctccgcc accttcgggc tcgacgtgct
      181 gccgccgggc ctgctgtacg cgagcatcgc gatgtgcccg accaccggcg gccgcgtcgc
      241 gagcttcgac gccacggccg cgcagaagct gccgggcgtg cgcaaggtga tggcgctgga
      301 gccggtcggc gccacgctca tcggcaccgg cgccacgccg ggcggggtgg ccgtcatcgc
      361 cgacacccct taccacgcga tgcgcgccgt caaggcgctc gccatcgaat gggatcacgg
      421 cccggccgcc agcctgtcga gctccgagat gatcgagcgc ctctcgcaga ccctgcgcac
      481 caggcccggc aacgcccggc tggacgacgg cgacgtggcc ggtgcgttcc gctcggccgc
      541 caagaccatc gaggccgagt accgtgtgcc cttcctggcc cacgcgacca tggagccgat
      601 gaattgcacc gtgcagttca aggacggcaa ggcgaccgtg tgggcgccca cccaggcgcc
      661 gggtttcacg cgcggtgcgg cggccaaggc gctgggcatc gacgcggaca aggtcgagct
      721 gcacgtgacc tacctcggcg gcggcttcgg ccggcgctac agcaccgact tcgtcaccca
      781 ggccgccacg ctggcgcgcg agaccggcgg cgcgccggtg cagctcttct ggtcgcgcga
      841 ggaagacatg gcgcacgact tctaccgccc cgcctacgtg gcgcgctgca gggcgggctt
      901 cgacgcggcc ggcgcgctgg tcgcgtggca gaccgtcacg gccggatcga gcatgggcgc
      961 cccctcgctc atggacaccg ccaccgacgg cgcctggaac acggcctatg ccttccccaa
     1021 cgcgcgcgtc gcccacgtgc cggtggagtc ggccatgccc acgggcgttt ggcgatcggt
     1081 cgcgcattcg cagaacggct tcttcgtcga gagcttcatc gacgagtgcg ccgccgccgc
     1141 gggcaaggac ccggtggcgt tccgcgccgc gctgctcgcg aaggacgagc gccacctgcg
     1201 cgtgctcagg cgcgtggccg aactctcgaa ctggagccag ccgccggcac cggggcccga
     1261 cggcgcgaag cgggcgcgcg gcctcgcgat ccaccgttcc ttcggcagca tcgtcgcgca
     1321 ggtggccgag gtatccgtct cgccggaccg gcaggtgcgg gtgcaccggg tgacctgcgt
     1381 cgtcgactgc ggcgtggccg tcaacccgaa cctgatccgc cagcagatgg aaggcgccat
     1441 cgtctacggc ctctccgccg cgctgcatgg cgagatcacg gtggagaagg gccgggtgca
     1501 gcagagcaac ttccacgact acatgccgct gcgcatgaac gagtgcccgg ccatcgaggt
     1561 cgagatcgcc gccagcggcg aagcgcccgg cggcgtgggc gagcccggca cgccgccgat
     1621 cgcgccggcg gtggcgaacg cggtgttcgc cctcacgggc cagcggctgc gcagcctgcc
     1681 gctccgcctc gcctgaacag ggaaaaaaga aagaaggagc accccatgac caccacgctc
     1741 acagtcaacg gcaaggccgt caccgtcaac gccgagcccg acacgccgct gctgtgggtg
     1801 ctgcgcggcg agatgcagct cgtcggcacc aagttcggct gcggcaaggc gctgtgcggc
     1861 gcctgcaccg tgcacctcga cggcgagccg gtgcgctcct gcgtcacgcc gctggaggcg
     1921 gtcggcagcc gcgaggtcac caccatcgaa ggcctcaagg gcagggaggc cgacgccctg
     1981 cgcaacgcct ggaccgaagt cgacgtggtg cagtgcggct actgccagag cggccagctc
     2041 atgtcggcct gcgcgttgct caaaaagacg ccgagcccaa gcgatgacga catttctggc
     2101 gccatgagcg gaaacatttg tcgctgtggc acctatccgc gcatccgcgc cgcgatccat
     2161 gtggctgccc ggaaagctgc aggctgacag cgaatgcaaa ggttctcgtc ttgacggtcg
     2221 gggtgggacg aggtaaggcc taaggtccca actggttcac acaggttaat aaattctccg
     2281 caatcatcga aaaagtccag gaataaaaat ttttacggaa actggggata tgaagctttt
     2341 gtacgcggcc gtaccatccg cgcggcccgc ttccggccgt ccccagtttc cactttcgat
     2401 gaccccccca gatccttcac ggacccgagc gttgtccgtg gccgataccc tgcgtgaccg
     2461 catcttcgac ggcgagatcg cacccggctc ccacctgatg gaaatctcgc tggccaacga
     2521 gctcggcgtg tcgcgcacgc ccgttcgcgg cgccatggca cggctggcgg acgaggggct
     2581 gctcgtctac ctgcccaaca agggcttcca ggtgcgccgc ttcaatgcca aggacgtgta
     2641 cgacgccttc agcgtgcgcg ccacgctgga gggcatgtgc
//
LOCUS       DATCRO010000761        14025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_31229, whole genome shotgun sequence.
ACCESSION   DATCRO010000761 DATCRO010000000
VERSION     DATCRO010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31229"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..818)
                     /locus_tag="VIN75_20645"
     CDS             complement(141..818)
                     /locus_tag="VIN75_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8976636.1"
                     /translation="MRLLLVEDNKVLADWLARTLRKQLYAVDWVENGADADFVLRTET
                     YDLVLLDLALPKLGGHEVLRRLRERGNTTPVLVLTADNTTRSRVTELDHGADDYVAKP
                     FDVDELEARIRVLLRRSAHVANPQIACGDLVFHGATREFTLTGKPLPLTPRERTVLEV
                     LVMKAGSTVSKAALGQSLSSLEDGVSADAIEIYVHRLRKKLEGSDATIVTLRGLGYVL
                     RPVHHGA"
     gene            complement(838..1854)
                     /locus_tag="VIN75_20650"
     CDS             complement(838..1854)
                     /locus_tag="VIN75_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976637.1"
                     /translation="MSIHTILRTTFARALAAAACALACAAPARAEPIQLMVGGIEKQI
                     YLPAALADRLGYFKEQGLDVQVLSDTSGGHAEDGLLTGSVQGVVGFYDHTIDLQAKGK
                     FVQAVVQFSQAPGEAVLVATRQADAIRSPVDFRGRSLGVTGMGSSTHLLMQYLAIENG
                     LKQGELRVSGVGSGDAFAEALRSGRIDVGMTTEPTASRLLKSGQARLLVDLRTPATAT
                     SALGGTYPAACLYMSAPWIAAHRGEVQRLVNALVKALRYIAAHSAQDIAAQLPADYLA
                     GDRALYVSTLQQSKSMFTADGVMPADGPANVLRVMRLAERAVTAKPIDLSRTYTTEFA
                     RSAR"
     gene            2075..3100
                     /locus_tag="VIN75_20655"
     CDS             2075..3100
                     /locus_tag="VIN75_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8976638.1"
                     /translation="MTNVISRREALLAAAAALAASAAGSARAQGAPIVIKFSHVVAPD
                     TPKGNGAQRFKELAEERTKGRVKVEVYPNSQLYKDKEEMEALQLGSVQMLAPSLAKFG
                     PLGVKEFEVFDLPFIFRNQAAFRAVTDGPVGADLFRKLEPKGIRGMAYWDNGFHIMSA
                     NRPLHRVADFKGLKMRIQSSKVLDAQMRALGAIPQVMAFSELYQALQSGVVDGTEGVP
                     SNFWTQKIYEVQKHMTLSYHGHLAYAVIVNKKFWDGLPEDLRTILDGCVRDATTYANA
                     LAATENAMALDRIRASGKTTVYTPTAAEIDEWRRALMPVHHEMASRVGQPTMDAVYKA
                     AGFVAPT"
     gene            3171..4613
                     /locus_tag="VIN75_20660"
     CDS             3171..4613
                     /locus_tag="VIN75_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8976639.1"
                     /translation="MTKFALRATALAVLALSQPAFANEIDDLKAEIAAQKAAAAAQQS
                     RLDALEQRLNAAQAQPAKPAVATTALKTDQNGNKLVETPAGVALYDDGNTSLHIYGLI
                     EATGSHANHQTTAGGSATGFQTAWFSGNRLGFDADHALAIGDSIGLPGLKVISKLETE
                     FELPTGDMDTSDVFFNRDAWMGFYSEDLGKVTLGRQNTLTRDFTANWGDPYGGADVSL
                     KEGGYSNVNNFKQFIFYSGGANGTRLNSAIEWKKKFGAHVVAGLGFAFGSQGAGGSGD
                     VGNGGSTPGDFKKGQTQEASIALNALPVGPGLLSANLSYDRANVNDLIHQSELVGGNY
                     RIGMFRVNAGYAHYTAEQGVGNSAGTRTDNSWTVSMSLLPTPENEIALGFNEMKGTHA
                     GFNGGGNILNPFGNTAGVSAVADGSKKAIYASWMYHVDKQFDVYLAGDHFKVDGGWVI
                     GDAQGNGNHFGAGQAYNNESEFAIGARYKF"
     gene            complement(4635..5627)
                     /locus_tag="VIN75_20665"
     CDS             complement(4635..5627)
                     /locus_tag="VIN75_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY8976640.1"
                     /translation="MTALRLVPWLLALPLLAHAAPPSLKTLDPIAVGGEARWDYPTID
                     SAAHRLYLSHATQVEVVDTRTGRLVGTVPDTAGVHGVAVAPDLGLGFASDGRADAVTV
                     FDLATLKLRATIPVGHGPDAILYDAASHRVMTFNAKSRDVTVIDATKGAVVATVPAGG
                     KPEFADAGAGGLVWFNVEDTNELAVLDPVAARIVKRIPLGPCEGPSGLAYDGRANHYA
                     VCDNGQMAVVDPEGRVVARPLIGAGPDGVAWLDGAAFSANGRDGTISVVRETHPGRFE
                     TVETIPTAAGARTIAADPATHRLYLPTAQFEPQAEGQRRKGVAGTFRVLVLEKP"
     gene            5991..7490
                     /locus_tag="VIN75_20670"
     CDS             5991..7490
                     /locus_tag="VIN75_20670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8976641.1"
                     /translation="MFRASVLSMAALMALAANAQAQVVKPGSLVVSRTMYAGDAGTVT
                     IGQALPGGGTAVADGAFPDVFKNESADAAFGVTAPIFLDTMTTSGTRTGTIAIDPNRL
                     STSFASKSELGLNISQDGKSVTFMGYLSPVNRLDVSNSNTPGVIDPTNAVKSVTQRGI
                     ATVSLSTGALTVTGINAYSGNNGRNVVLAGGNYYTVGNAGNSGKGVSGTVLGHLSDDT
                     GLQMIAAGQSGATTVVGQAYGSFGNTTGYQHGFSTVATGGAADKTGKDDNFRGETLFD
                     NTLYVTKGSGGNGVNTVFQVGTAGRLPTAATASSTAITPLAGFPTSGTATHPFGLWFA
                     NATTLFVADEGDGQAPGTTGKDTSNAGLDEYTFTGGRWNEVAVFQKGLLDQAAYSDGL
                     PWQVSTDGLRNLAGRVNSDGSFTLFATTSTVSNEGTHDQGADPNELVSITIGAGSTPL
                     NDSFTVLETAGYGERLGGVALTAVPEPGNVALLLAGLGVVGLRLRRRAR"
     gene            7555..8238
                     /locus_tag="VIN75_20675"
     CDS             7555..8238
                     /locus_tag="VIN75_20675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976642.1"
                     /translation="MSDAHPLDNVIWNALCTVHAGLSRGGPLARRYQPDFARFAGLPA
                     ATPQAFAALADDLAAGEVVALSMPADVDPGPAFEVLDRKDLVQMTGAVTGDVAQPDRF
                     RALGPQDVPQMTALVQLTAPGPWYERTAELGRFVGIEVDGRLVAMTGERMRVPGHTEI
                     SAVCCHPDFRGRGWPADLMRLVSRAIVARGETPFLHALAENAPAIALYEKLGMRKRLS
                     SRLLILQRV"
     gene            complement(8241..8519)
                     /locus_tag="VIN75_20680"
     CDS             complement(8241..8519)
                     /locus_tag="VIN75_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976643.1"
                     /translation="MTANTPIPHRDERTRDDLPDGRNGRVVGESDDNVLESIGKAVVA
                     PVEGADEGAEPVDGNHGRAFVDLTRTLPQGQQAAPRTPAAPKPDGKAL"
     gene            8637..9023
                     /locus_tag="VIN75_20685"
     CDS             8637..9023
                     /locus_tag="VIN75_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY8976644.1"
                     /translation="MQIQVNADNHIEASEARNVWARSVVESALAHLADSVTRVEVHLS
                     DESAGKGGAPALRCTMEARLNGRPPIAVSHDAAGMDAAINGAVHKLVRATAHALGRAD
                     KHAHAERALPAGDTGDDDFVPSRAPF"
     gene            complement(9020..9988)
                     /locus_tag="VIN75_20690"
     CDS             complement(9020..9988)
                     /locus_tag="VIN75_20690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8976645.1"
                     /translation="MDSQHATLDPQSHTWLELGEVQQLLLDAYPGVVLGVDGHSRIRW
                     INPVAADRLGYARDELAGRPIAGSLISQEELEARAAELSAILGEHVVPDAGVLGTALT
                     RDGVPSEHDWMLRHKDGSSHSTRLNVGALRDHRGQVVGLIAVEPLHQGEGAPLRLTHH
                     DALTGLPTRAVLQDRAEMALLRAARQKTVLAFMLIEIDGFDALCEKYGRTVGDDVLRA
                     TAGRLHFELRKTDTAVRLDHGQFAAMLVDLHHAEEAQRVAEKVAQALAAPVNVGVARL
                     ALAARVGVVWSPDHGNQLVPLLQAAEAALATLPAGQGGVAAGDPRR"
     gene            10176..10988
                     /locus_tag="VIN75_20695"
     CDS             10176..10988
                     /locus_tag="VIN75_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8976646.1"
                     /translation="MEAFALGALVGLLLALTGAGGGILAVPLLVFALHLGLAEAAPIG
                     LLAVGLAAAMSAALGLRDGIVRYRAAALMGGLAMGLAPLGVAAAQRVPNAPLTLAFAA
                     VLALSAWRMWQRAKVALTPRGGIARAALPCVLDARDGRLVWTRPCAQALALTGVAAGL
                     LSGLLGVGGGFVIVPALTRFTELSARSIVATSLAVIALASIGGVGAAAWHGAIAWAIA
                     LPFAVGSVGASLLARAIASRVAGPRLQQGFAIVSLGVALLLAARVAGVLGLR"
     gene            complement(10985..11446)
                     /locus_tag="VIN75_20700"
     CDS             complement(10985..11446)
                     /locus_tag="VIN75_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8976647.1"
                     /translation="MDAIDRKLLEMLQLDCDTPIAELAAAVQLSTTPCWRRVQRMKDA
                     GVITGNVALVDPKSVNVGVTVFVNIRTNQHSQAWFDRFHATVESIPEVVEFYRMSGDV
                     DYLLRIVVPDIAAYDGVYKRLIAGTQLFDVSSSFAMEQLKFTTALPLRYAD"
     gene            11562..12776
                     /locus_tag="VIN75_20705"
     CDS             11562..12776
                     /locus_tag="VIN75_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976648.1"
                     /translation="MTVSPSLAAHPSFLRFWAGRVAGTMGNQMVMVAVGWQMYDLTHS
                     AWALGLVGLFQFVPALALALVSGHVADHHDRRHIVSMAMSLQLAAALMLLLSTHEGWI
                     SRDLILGVSLLLGVARSFQMTAQQALTPLLVPASILSRAMAFSSSGNQAAIIGGPALG
                     GFLYAAGASVVYVVCAGLFVVGASLVWSARYDHRPAPRAPVTMDTLLAGVRFIRAKPV
                     VLGAISLDLFAVLLGGAVALLPIYARDILHVGPEGLGLLRGAPAVGALLMSLVLARWT
                     IRSRAGAILFSCVAIFGLTTIVFGLSTSFWLSMAMLFINGAVDMVSVVVRQTLVQLDT
                     PDEMRGRVSAVNAIFIGASNQLGEFESGATAALFGPVGAVVAGGVGTLVVVGLWMRWF
                     PELLRRRSLTEA"
     gene            complement(12963..13541)
                     /locus_tag="VIN75_20710"
     CDS             complement(12963..13541)
                     /locus_tag="VIN75_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8976649.1"
                     /translation="MPVSPDEARLVALARATPWFMDALAHVRALRLPAWCVGAGAVRN
                     LVWDALHGHAVSSALADIDVAHFDAADLSAERDTLLQRRLHAMAPALPWEVTNQAGVH
                     LWFEQHFGHAVGPLACLADAVASWPEFATCVGLWLDDDDALHVIAPHGLDDLFAMVVR
                     RNPARVSVQTYRERVASKRYAQRWPSVTIVEA"
     gene            complement(13541..>14025)
                     /locus_tag="VIN75_20715"
     CDS             complement(13541..>14025)
                     /locus_tag="VIN75_20715"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078396.1"
                     /note="catalyzes the formation of
                     2-dehydro-3-deoxy-D-octonate 8-phosphate from
                     phosphoenolpyruvate and D-arabinose 5-phosphate in LPS
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY8976650.1"
                     /translation="VVALAKTGRVINIKKPQFLSPSQMANICEKFREAGNDQRILCDR
                     GTCFGYDNLVVDMLGFSVMKKTTGDAPVIFDVTHSLQQRASGAAASGGRREQVAELAR
                     SGLALGLGGLFLESHPEPDKALCDGPSALPLDQLEPFLAQMKAIDDLVKSFPPLDIHG
                     "
BASE COUNT         2029 a         4998 c         4983 g         2015 t
ORIGIN      
        1 cgatgcgggc cgagccgagc agcaggcgat cctgcaccac cgccgccgtg gcctcggcgt
       61 tctggcggct gacgatgccg tcgaacacga ccgcccccgc gagcggcagc atcacccagg
      121 ccagcagccg gccgcgcagg ctatgcgcca tggtggacgg ggcgcagcac gtagcccagg
      181 ccgcgcagcg tgacgatggt ggcgtcgctg ccctcgagct tcttgcgcag gcggtgcacg
      241 tagatctcga tcgcgtcggc cgagacgccg tcctccagcg acgagaggct ctggcccagc
      301 gccgccttcg acacggtgga gcccgccttc atgacgagca cctcgagcac ggtgcgctcg
      361 cgcggcgtca gcggcagcgg cttgccggtc agcgtgaact cgcgcgtggc gccgtggaag
      421 acgaggtcgc cgcaggcgat ctgcgggttg gccacgtgcg ccgagcggcg cagcagcacg
      481 cggatgcgcg cctcgagctc gtcgacgtcg aacggcttgg cgacgtagtc gtccgcgccg
      541 tgatcgagct cggtgacgcg gctgcgcgtg gtgttgtcgg cggtcagcac gagcacgggc
      601 gtggtgttgc cgcgttcgcg caggcggcgc agcacctcgt ggccgcccag cttgggcagc
      661 gccaggtcga gcagcacgag gtcgtaggtc tcggtgcgca gcacgaagtc ggcgtcggcg
      721 ccgttctcga cccagtcgac ggcgtagagc tgcttgcgca gcgtgcgcgc gagccagtcc
      781 gccagcacct tgttgtcctc cacgagcagc agtcgcatgg cgcagggtcg ggtggcgtca
      841 gcgcgcggac cgcgcgaact cggtggtgta cgtgcgcgac aggtcgatcg gcttggcggt
      901 gaccgcgcgt tccgccagcc gcatcacgcg cagcacgttg gccgggccgt cggccggcat
      961 cacgccgtcg gcggtgaaca tcgacttgct ctgctgcagc gtgctcacgt acagcgcgcg
     1021 gtctcccgcg aggtagtcgg ccggcagctg cgccgcgatg tcctgcgcgc tgtgcgcggc
     1081 gatgtagcgc agcgccttga ccagcgcgtt gaccagccgc tgcacctcgc cgcgatgcgc
     1141 ggcgatccag ggcgccgaca tgtacaggca ggccgccgga taggtgccgc ccagcgcgct
     1201 cgtcgccgtg gcgggcgtgc gcaggtcgac cagcagccgg gcctggcccg acttgagcag
     1261 ccgcgacgcg gtgggctcgg tcgtcatgcc cacgtcgatg cggcccgagc gcagcgcctc
     1321 ggcgaacgcg tcgccggagc ccacgcccga gacgcgcagc tcgccctgct tgagcccgtt
     1381 ctcgatcgcc aggtactgca tcagcaggtg cgtggacgaa cccatcccgg tgacgccgag
     1441 cgagcggccg cggaaatcga ccggcgagcg gatcgcgtcg gcctgcctgg tcgccaccag
     1501 cacggcctcg cccggcgcct ggctgaactg caccacggcc tgcacgaact tgcccttggc
     1561 ctgcaggtcg atcgtgtggt cgtagaagcc gacgacgccc tgcacgctgc cagtgagcag
     1621 gccgtcttcc gcatggccgc cggaggtgtc gctcagcacc tgcacgtcca ggccctgttc
     1681 cttgaagtag ccgagccggt cggccagcgc cgcgggcagg tagatctgct tctcgatgcc
     1741 gcccaccatc agctggatcg gctccgcgcg cgcgggcgcc gcgcacgcga gcgcgcaggc
     1801 ggccgcggcc agcgcgcgcg cgaaggtcgt ccgcaggatc gtgtggatgc tcatggctgt
     1861 ctcgccagtc gttgtaaggg gcccgtctcc agatcgaata ctacgagcgc acggcgtgcg
     1921 ccgcacctgt cgcgatccct tcgttgtttg agcgactacg tggtttccct cgacctgtcg
     1981 ggctgaaagc tgttggaagg gtgggggctc atagcctcgt cgacagacgg ggggacgcca
     2041 tgacgtccgc cccgcaacca cggagacaga ccccatgaca aacgtcatct cgcgccgcga
     2101 ggccctcctc gcggcagccg ctgcactggc cgcttcggcc gcgggcagcg cccgcgccca
     2161 gggcgcgccc atcgtcatca agttcagcca cgtcgtcgcg ccggacacgc ccaagggcaa
     2221 cggcgcgcag cgcttcaagg agctcgccga ggagcgcacg aaagggcgcg tgaaggtcga
     2281 ggtctacccg aacagccagc tgtacaagga caaggaggag atggaggccc tgcagctcgg
     2341 atcggtgcag atgctcgccc cgtccctcgc caagttcggg ccgctggggg tcaaggagtt
     2401 cgaggtcttc gacctgccgt tcatcttcag gaaccaggcc gcgttccgcg ccgtgaccga
     2461 cggccccgtg ggcgccgacc tgttccgcaa gctcgaaccc aagggcatcc gcggcatggc
     2521 gtactgggac aacggcttcc acatcatgag cgcgaaccgg ccgctgcacc gcgtggccga
     2581 cttcaagggc ctgaagatgc gcatccagtc gtccaaggtg ctcgacgcgc agatgcgcgc
     2641 gctcggcgcg atcccgcagg tgatggcctt cagcgagctg taccaggcgc tgcagtcggg
     2701 cgtcgtcgac ggcaccgagg gcgtgccgtc gaacttctgg acgcagaaga tctacgaggt
     2761 gcagaagcac atgacgctct cgtaccacgg gcacctcgcc tacgcggtga tcgtcaacaa
     2821 gaagttctgg gacggcctgc ccgaggacct gcgcacgatc ctggacggct gcgtccggga
     2881 cgcgacgacc tacgccaacg cgctggccgc caccgagaac gcgatggcgc tggacaggat
     2941 ccgcgcgagc ggcaagacca cggtctacac gcccaccgcc gccgagatcg acgagtggcg
     3001 ccgggcgctg atgcccgtcc accacgagat ggcctcgcgc gtcggccagc ccacgatgga
     3061 cgccgtctac aaggccgccg gcttcgtcgc gccgacctga gttcacgcat ctggacttgc
     3121 ccccagacga ccacccgccc cgcgaagggg tgacaaccac cggagacacc atgaccaagt
     3181 tcgcattgcg cgcgaccgcg ctggccgtgc tcgccctgag ccagcccgcg ttcgccaacg
     3241 agatcgacga cctcaaggcg gagatcgccg cgcagaaggc ggcggccgcg gcgcagcaat
     3301 cccgactcga cgcgctggag cagcgcctga acgccgcgca ggcccagcct gccaagcccg
     3361 cggtcgcgac gactgcactc aagaccgacc agaacggcaa caagctggtc gagacgccgg
     3421 ccggcgtcgc gctctacgac gacggcaaca ccagcctgca catctacggc ctgatcgagg
     3481 cgaccggcag ccacgccaac caccagacca cggccggcgg ctcggccacc ggcttccaga
     3541 ccgcctggtt cagcggcaac cgcctgggct tcgacgccga ccacgcgctg gccatcggcg
     3601 acagcatcgg cctgccgggc ctgaaggtga tctccaagct cgagaccgag ttcgagctgc
     3661 ccaccggcga catggacacc tccgacgtgt tcttcaaccg cgatgcgtgg atgggcttct
     3721 acagcgagga cctgggcaag gtcaccctcg gccgccagaa cacgctgacg cgcgacttca
     3781 cggccaactg gggcgacccg tacggcggcg ccgacgtgtc gctcaaggaa ggcggctact
     3841 ccaacgtcaa caacttcaag cagttcatct tctactcggg cggcgccaac ggcacgcgcc
     3901 tgaacagcgc gatcgagtgg aagaagaagt tcggcgcgca cgtcgtggcc ggcctcggct
     3961 tcgcgttcgg ctcgcagggc gccggcggca gcggcgacgt cggcaacggc ggctcgacgc
     4021 ccggcgactt caagaagggc cagacgcagg aagcctcgat cgcgctgaac gcgctgccgg
     4081 tcggcccggg cctgctcagc gccaacctgt cgtacgaccg cgccaacgtc aacgacctga
     4141 tccaccagtc cgagctggtc ggcggcaact accgcatcgg catgttccgc gtgaacgccg
     4201 gctatgcgca ctacacggcc gagcagggcg tcggcaacag cgccggcacc cgcaccgaca
     4261 actcgtggac ggtgtcgatg agcctgctgc ccacgcccga gaacgagatc gcgctgggct
     4321 tcaacgagat gaagggcacg cacgccggct tcaacggcgg cggcaacatc ctcaacccgt
     4381 tcggcaacac cgcgggcgtc agcgccgtgg ccgacggctc gaagaaggcg atctacgcgt
     4441 cgtggatgta ccacgtcgac aagcagttcg acgtctacct tgccggcgac cacttcaagg
     4501 tggatggcgg ctgggtgatc ggcgacgcgc agggcaacgg caaccatttc ggcgccggcc
     4561 aggcctacaa caacgagagc gagttcgcga tcggcgcgcg ctacaagttc tgacgcgcga
     4621 gcggggcagc gtgctcaggg cttctccagc acgagcacgc ggaaggtgcc ggcgacgccc
     4681 ttgcgccgct ggccctcggc ctgcggctcg aactgcgcgg tcggcaggta gagccggtgc
     4741 gtcgccggat ccgcggcgat ggtgcgcgcg ccggccgcgg tcgggatcgt ctcgacggtc
     4801 tcgaagcggc cggggtgcgt ctcgcgcacg acgctgatcg tgccgtcgcg gccgttggcg
     4861 ctgaaggccg ccccatccag ccaggcgacg ccgtccgggc cggcgccgat gagcggccgg
     4921 gcgacgacac ggccttcggg gtcgaccacc gccatctgac cgttgtcgca cacggcgtag
     4981 tggttcgcac ggccgtcgta ggcgaggccg ctcgggccct cgcacggccc cagcgggatg
     5041 cgcttgacga tgcgtgccgc gacggggtcg agcacggcca gctcgttcgt gtcctcgacg
     5101 ttgaaccaca ccaggccgcc ggcgcccgcg tcggcgaatt cgggcttgcc gcccgcgggc
     5161 acggtggcca cgaccgcgcc cttggtcgcg tcgatcacgg tgacgtcgcg cgacttcgcg
     5221 ttgaacgtca tcacgcgatg gctcgcggcg tcgtacagga tcgcgtccgg gccgtggccg
     5281 acggggatcg tcgcgcgcag cttgagcgtc gccaggtcga agacggtcac cgcgtcggcg
     5341 cggccgtcgc tcgcgaaccc gaggcccagg tccggggcga ccgcgacgcc atggacgccg
     5401 gcggtgtcgg gcacggtgcc gaccagcctg ccggtgcgcg tgtccaccac ctcgacctgc
     5461 gtggcatgcg acaggtacag ccggtgcgcg gcactgtcga tggtcggata gtcccagcgc
     5521 gcctcgccgc cgacggcgat cgggtccagc gtcttgagag acggcggcgc ggcatgggcg
     5581 agcagcggca gggcgagcag ccaggggacg agtcggagtg cggtcatggg cgcactctag
     5641 cgcgcgccgc ggcgcggcgc gccacgccac cgcggggtcc gccaccgcgg ggtcaggtct
     5701 tgaatgttgc ggggtcaggt cttgagtgcg cgattcaaga cctgaccccg caacattcaa
     5761 gacctgaccc catgccgcgg acccaatgcc gcggtcgctc ggctgtcatg cggatgaagg
     5821 cgcggcgcaa ggctcgcgcc agcctcgccg ccgtgtcact gcgccgtcac ggccgcttcc
     5881 taccgtgcgg ggcttcgggc ggctcgacgc cggcgcgaag ggcgacagcc cgatcccgcg
     5941 caggccgtcc cgcgcccagc catccccgtc cacaagaagg aaatccgcgc atgttccgtg
     6001 caagtgtcct gtccatggcc gcgctcatgg cgctcgccgc caacgcgcaa gcccaggtcg
     6061 tcaagcccgg cagcctcgtc gtctcgcgca ccatgtacgc gggcgacgcc ggcaccgtca
     6121 ccatcggcca ggcgctgccc ggcggcggca cggcggtcgc cgacggtgcg ttccccgacg
     6181 tgttcaagaa cgagagcgcc gacgcggcct tcggcgtcac cgcgccgatc ttcctcgaca
     6241 cgatgaccac gtcgggcacg cgcaccggca ccatcgcgat cgacccgaac cggctcagca
     6301 cgagcttcgc atcgaagtcg gagctgggcc tgaacatctc gcaggacggc aagtcggtga
     6361 ccttcatggg ctacctctcg ccggtcaacc gcctcgacgt ctcgaactcc aacacgcccg
     6421 gcgtgatcga cccgaccaac gcggtgaagt cggtgacgca gcgcggcatc gccaccgtga
     6481 gcctgtccac cggcgcgctg acggtgaccg gcatcaacgc ctacagcggc aacaacggtc
     6541 gcaacgtggt gctggccggc ggcaactact acaccgtcgg caatgccggc aactcgggca
     6601 agggcgtcag cggcaccgtg ctgggccacc tgagcgacga caccggcctg cagatgatcg
     6661 cggccggcca gtcgggcgcc accaccgtcg tcgggcaggc ctacggcagc ttcggcaaca
     6721 cgaccggcta ccagcacggc ttctccacgg tcgccacggg cggcgcggcc gacaagaccg
     6781 gcaaggacga caacttccgc ggcgaaacgc tgttcgacaa cacgctctac gtgaccaagg
     6841 gcagcggcgg caacggcgtc aacaccgtct tccaggtggg caccgcgggc cggctgccga
     6901 cggccgccac cgcctcgtcg accgcgatca cgccgctcgc gggattcccg acctccggca
     6961 ccgcgactca cccgttcggc ctgtggttcg ccaacgccac cacgctgttc gtcgccgacg
     7021 agggcgacgg ccaggcgccg ggcaccacgg gcaaggacac gtccaacgcg ggcctggacg
     7081 aatacacgtt cacgggcggc aggtggaacg aggtcgccgt gttccagaag ggcctcctcg
     7141 accaggccgc ctacagcgac ggcctgccct ggcaggtgag caccgacggc ctgcgcaacc
     7201 tcgcgggccg ggtcaattcc gatggctcgt tcacgctgtt cgcgacgacg tcgacggtca
     7261 gcaacgaagg cactcacgac cagggcgcgg atccgaacga gctggtctcg atcacgatcg
     7321 gcgcgggctc cacgccgctg aacgattcgt tcacggtgct ggagaccgcc ggctacggcg
     7381 agcgcctggg cggcgtcgcg ctgacggccg tgccggagcc gggcaacgtc gcgctgctgc
     7441 tggccggcct gggcgtggtc gggctgcgcc tgcgtcgccg cgcccgctga ggcggcgcgt
     7501 cacggcaagg ccccacgtcg acaccggcgt cggcgcggcg gcacactgtc gcgcatgagc
     7561 gacgcccatc ccctcgacaa cgtcatctgg aacgcgctgt gcaccgtgca cgcggggctg
     7621 tcgcgcggcg gcccgctcgc gcggcgctac cagccggatt tcgcgcgctt cgccggcctg
     7681 ccggccgcga cgccgcaggc tttcgcggcg ttggcggacg acctggcggc gggcgaggtg
     7741 gtggcgctgt cgatgccggc cgacgtcgat ccgggtcccg cgttcgaggt gctcgatcgc
     7801 aaggacctgg tgcagatgac cggcgcggtc accggcgacg tggcgcaacc cgatcgcttc
     7861 cgcgcgctgg ggccgcagga cgtgccgcag atgacggcgc tcgtgcagct gaccgcgccc
     7921 ggcccgtggt acgaacgcac cgcggagctc ggacgcttcg tcggcatcga ggtcgacggc
     7981 cgcctggtcg cgatgaccgg cgagcgcatg cgcgtgccgg ggcacaccga gatcagcgcc
     8041 gtctgctgcc accccgactt ccgcggccgc ggctggccgg ccgacctgat gcgcctcgtg
     8101 tcgcgcgcga tcgtcgcgcg cggcgagacg ccgttcctgc acgcgctcgc cgagaacgcg
     8161 ccggcgatcg cgctgtacga gaagctcggg atgcgcaagc gcctgagctc gcggctgctg
     8221 atactccagc gggtatagcg tcagagcgcc ttgccgtccg gcttgggcgc cgctggcgtg
     8281 cgtggcgcgg cctgctgccc ctgcggcagc gtcctggtca ggtcgacgaa cgcgcggccg
     8341 tggttgccgt cgaccggctc ggcgccttcg tcggcaccct cgaccggcgc gacgacggcc
     8401 ttgccgatcg actcgaggac gttgtcgtcg ctctcgccga cgacgcggcc gttgcggccg
     8461 tcaggcaggt cgtcgcgggt gcgctcgtcg cgatgcggga tgggggtgtt ggcggtcatg
     8521 gtcatgtccc tgggtggatg accgtggtcg ggcaatcgcc atgccccggc gcgcggccgc
     8581 gccgatgggc acgaggactg ccgcaacgac cctggaagaa cttcaaggga cacgacatgc
     8641 agatccaggt caacgccgac aaccacatcg aggccagcga ggcccgcaat gtctgggcgc
     8701 gcagcgtcgt cgagagcgcg ctcgcgcatc tggccgacag cgtgacgcgc gtggaggtgc
     8761 acttgagcga cgagagcgcc ggcaagggcg gggcgcccgc attgcgctgc acgatggagg
     8821 cgcgcctgaa cggccgtccg ccgatcgcgg tgagccacga cgccgcgggc atggacgcgg
     8881 ccatcaacgg cgcggtgcac aagctcgtgc gcgcgacggc ccacgcgctc ggccgcgcgg
     8941 acaagcacgc gcacgcggaa cgcgcgttgc ccgcgggcga caccggcgac gacgacttcg
     9001 tgccgtcgcg cgccccgttc tagcgtcgcg gatcgcccgc ggcgacgccg ccctggccgg
     9061 ccggcagcgt ggccagcgcg gcctcggcgg cctgcagcag cggcaccagc tgattgccgt
     9121 ggtcgggcga ccagacgacg cccacgcgcg ccgccagcgc cagccgcgcg acaccgacgt
     9181 tgaccggcgc cgccagcgcc tgcgccacct tctcggcgac gcgctgcgcc tcctcggcgt
     9241 ggtgcaagtc gaccagcatc gcggcgaact ggccgtgatc gaggcgcacg gccgtgtcgg
     9301 tcttgcgcag ctcgaagtgg aggcgacccg cggtggcgcg cagcacgtcg tcgccgacgg
     9361 tgcggccgta cttctcgcac agcgcgtcga agccgtcgat ctcgatgagc atgaaggcca
     9421 gcaccgtctt ctgccgcgcg gcgcgcagca gtgccatctc ggcgcgatcc tgcagcacgg
     9481 cgcgcgtggg caggccggtg agggcgtcgt ggtgcgtgag gcgcagcggc gcgccttcgc
     9541 cctggtgcag cggttcgacg gcgatcaggc cgacgacctg gccgcggtgg tcgcgcagcg
     9601 cgcccacgtt cagccgcgtg gagtgcgacg agccgtcctt gtggcgcagc atccagtcgt
     9661 gctcgctggg gacgccgtcg cgcgtgagcg ccgtgccgag cacgccggcg tcgggcacca
     9721 cgtgctcgcc gaggatggcc gacaactcgg ccgcccgcgc ctcgagttcc tcctgggaga
     9781 tcaggctgcc ggcgatgggc cgcccggcga gttcgtcgcg ggcgtagccc agccggtcgg
     9841 cggccaccgg gttgatccag cgtattcggc tgtgcccgtc gacccccagc acgactccgg
     9901 ggtaggcatc gagcagcagc tgctgcactt ctccgagttc cagccaggtg tgggactgcg
     9961 gatccagggt ggcgtgttgg ctgtccatcg ggccagtctc tcaccgaccg ggcgtcggcg
    10021 acaagtcccc gcgcctgcgt gtcaacccga tggctgcccg ctgcaatgtc gcgtaccgtg
    10081 cgcgctgttc aaatacgcgt catgaccatc gtctccgcct cgatcgtccg cacccggacg
    10141 ccgaagcgct tcgacgccga ccacgagacg gcgtgatgga agccttcgca ctcggcgccc
    10201 tggtgggact gctgctcgcg ctcaccggcg caggcggcgg catcctcgcc gtgcccctgc
    10261 tcgtcttcgc cctgcacctg ggcctggccg aggccgcgcc catcggcctg ctggccgtgg
    10321 gcctggccgc ggcgatgtcg gccgcgctcg gcctgcgcga cggcatcgtg cgctatcgtg
    10381 ccgccgcgct gatgggcgga ctcgcgatgg ggctcgcgcc cctgggcgtg gccgccgcgc
    10441 agcgcgtgcc caacgcgccg ctcacgctcg cgttcgccgc ggtgctggcg ctgtcggcgt
    10501 ggcgcatgtg gcagcgcgcg aaggtcgcgc tgacgccgcg cggagggatc gcgcgcgcgg
    10561 cgctcccgtg cgtgctcgac gcgcgcgacg gccggctcgt gtggacgcgc ccgtgcgcgc
    10621 aggcgctcgc gctcaccggc gtggccgcgg gcctgctcag cggcctgctg ggcgtgggcg
    10681 gcggcttcgt catcgtgcct gcgctcacgc gcttcacgga gctgtcggcg cgcagcatcg
    10741 tcgccacgtc gctggcggtc atcgcgctcg cgtcgatcgg cggcgtgggc gccgcggcgt
    10801 ggcatggcgc gatcgcgtgg gccatcgcgc tcccgttcgc cgtcggctcg gtgggcgcct
    10861 cgctgctcgc gcgcgccatc gcgtcgcgcg tcgcggggcc gcgactgcag cagggcttcg
    10921 cgatcgtcag cctgggcgtg gcgctgttgc tggccgcccg ggtggccggt gtgctcggcc
    10981 tgcgctagtc cgcgtagcgc aacggcaggg cggtggtgaa cttcagctgc tccatcgcga
    11041 agctcgaact gacgtcgaac agctgcgtgc cggcgatcag ccgcttgtag acgccgtcgt
    11101 aggccgcgat gtcgggcacg acgatgcgca gcaggtagtc gacgtcgccg ctcatgcgat
    11161 aaaactcgac gacctcgggg atcgattcga cggtcgcatg gaagcgatcg aaccaggcct
    11221 ggctgtgctg gttggtccgg atgttgacga acaccgtcac gcccacgttg acgctcttcg
    11281 gatcgaccag cgccacgttg ccggtgatca cgccggcgtc cttcatgcgc tggacgcggc
    11341 gccagcaggg cgtcgtcgag agctgcaccg cggcggcgag ctcggcgatt ggggtgtcgc
    11401 aatcgagctg cagcatctcc agcagtttgc ggtcgatggc atccagtttc atgtcgaggc
    11461 cttcacgaag aacgaaattc tatttcgtgg cgaagcgctc cggggcggaa ttcaaccccg
    11521 ggccagctcg gtgaaggccg atgtgggacg atgtcgagct gatgactgtg tccccttcgc
    11581 tcgccgcgca cccttccttt ctccgcttct gggcaggccg cgtggccggc acgatgggca
    11641 accagatggt gatggtggcc gtcggctggc agatgtacga cctgacgcac agcgcgtggg
    11701 ctctcggcct cgtcggcctg ttccagttcg tgccggccct cgcgctggcc ctcgtctcgg
    11761 gccacgtcgc cgaccaccac gaccgccgcc acatcgtgtc gatggcgatg tcgctgcagc
    11821 tggccgcggc gctgatgctg ctgctgtcaa cgcacgaagg gtggatctcg cgcgacctga
    11881 tcctgggcgt gtcgctgctg ctgggcgtgg cgcgctcgtt ccagatgacg gcgcagcagg
    11941 cgctgacgcc gctgctggtg cccgcgtcca tcctgtcgcg cgcgatggcc ttcagctcct
    12001 cgggcaacca ggccgccatc atcggcggcc cggcgctcgg cggcttcctg tatgccgcgg
    12061 gcgccagcgt ggtgtacgtc gtctgcgcgg ggctgttcgt cgtcggcgcc agcctcgtgt
    12121 ggagcgcgcg ctacgaccac aggcccgccc cgcgcgcgcc cgtgacgatg gacacgctgc
    12181 tggccggcgt gcgcttcatc cgcgccaagc cggtggtgct gggcgcgatc tcgctcgacc
    12241 tgttcgcggt gctgctgggc ggcgccgtgg cgctgctgcc gatctacgcg cgcgacatcc
    12301 tgcacgtcgg cccggagggc ctggggctgc tgcgcggcgc gccggcggtg ggcgcgctgc
    12361 tgatgtcgct ggtgctggcg cgctggacga tacgctcgcg cgccggcgcg atcctgttct
    12421 cgtgcgtggc gatcttcggc ctgacgacga tcgtgttcgg cctgtccacc tcgttctggt
    12481 tgtcgatggc gatgctgttc atcaacggcg cggtcgacat ggtcagcgtg gtcgtgcgcc
    12541 agaccctggt gcagctcgat acgcccgacg agatgcgcgg ccgcgtcagc gcggtcaacg
    12601 cgatcttcat cggcgcgagc aaccagctgg gcgagttcga gtcgggcgcg acggcggcgc
    12661 tgttcgggcc tgtcggcgcg gtcgtcgccg gcggcgtggg cacgctggtg gtcgtcgggc
    12721 tgtggatgcg ctggttcccg gagctgctgc gccggcgcag cctgaccgag gcgtgatcgg
    12781 catcaggccg acgacaggct cggcccttcg cgcgcagagg ccgctgcaat ggggtccgct
    12841 gcaatggggt caggtcttga aagttgcgcg acggtgcttc ggcgaccggc cgtcaggcaa
    12901 gcgcgcaact ttcaagacct gaccccgcgg cgacggaccc cacggcgacg gagttccggg
    12961 gatcacgcct cgacgatcgt gacgctcggc cagcgctgcg cgtagcgctt ggacgccacg
    13021 cgctcgcgat aggtctggac gctgacacgc gccggattgc gtcgcaccac catcgcgaac
    13081 aggtcatcga ggccgtgcgg tgcgatcacg tgcagcgcgt cgtcgtcgtc cagccacagg
    13141 cccacgcagg tcgcgaactc gggccagctg gccaccgcgt cggccaagca agccaacggg
    13201 ccgaccgcat ggccgaagtg ctgctcgaac cacagatgca cgcccgcctg gttcgtgacc
    13261 tcccacggca gcgctggcgc catcgcgtgc aggcgccgct gcagcagcgt atcgcgctcg
    13321 gccgagaggt cggcggcgtc gaaatgcgcg acgtcgatgt cggccagcgc cgacgacacg
    13381 gcgtgcccgt gcagcgcgtc ccacacgagg ttgcgcaccg cgcccgcgcc cacgcaccac
    13441 gcgggcaggc gcagcgcgcg cacgtgcgcg agggcgtcca tgaaccacgg cgtggcgcgc
    13501 gccagcgcga cgaggcgagc ctcgtcgggc gacaccggca tcaaccgtgg atgtccagcg
    13561 gcgggaacga cttgaccagg tcgtcgatcg ccttcatctg agcgaggaaa ggctcgagct
    13621 ggtcgagcgg cagcgcgctc gggccgtcgc acagcgcctt gtccggctcg ggatgcgact
    13681 ccaggaacag accgcccagg cccagcgcca ggcccgagcg cgcgagctcg gccacctgtt
    13741 cgcggcggcc gcccgacgcg gcggcgccgg aggcgcgctg ctgcaaggag tgggtgacgt
    13801 cgaagatcac cggcgcgtcg cccgtcgtct tcttcatcac ggaaaagccc agcatgtcga
    13861 cgaccaggtt gtcgtagccg aagcaggtgc cgcggtcgca caggatccgc tggtcgttgc
    13921 cggcctcgcg gaacttctcg cagatgttgg ccatctgcga cgggctcagg aactgcggct
    13981 tcttgatgtt gatgacgcgg ccggtcttcg ccagcgccac gacca
//
LOCUS       DATCRO010000762         5085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_987329, whole genome shotgun sequence.
ACCESSION   DATCRO010000762 DATCRO010000000
VERSION     DATCRO010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987329"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VIN75_20720"
     CDS             complement(<1..97)
                     /locus_tag="VIN75_20720"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618138.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8976651.1"
                     /translation="MTATLQISRDGAVARVFLNRPDVRNAFNDAVI"
     gene            complement(108..776)
                     /locus_tag="VIN75_20725"
     CDS             complement(108..776)
                     /locus_tag="VIN75_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976652.1"
                     /translation="MNQLRIPSFPTRATPAVLAVAAALSVLAPPAQARNVRLMESVPD
                     TIRVTRTHDILGNLDLRFGSDTARGASIVRHDVVVKGVGTTRVKDVGDDDRVRPTDHD
                     LCARAFEDAITQLAFAARGARASAIVGVVSNYEGSVFDDPTHFECHAGALKAAVTLRA
                     QLAVGPVAPTPPSEVPVVSPPPQPVSPAVPVISPPQPRSDLVPPPPPPTFQPLPPAPA
                     PASN"
     gene            complement(886..1719)
                     /locus_tag="VIN75_20730"
     CDS             complement(886..1719)
                     /locus_tag="VIN75_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976653.1"
                     /translation="MLRSVPLSLLISAAVLAVARPALARNETFTLSVHDAFHKNRARE
                     IVGDMPVRFGAASAPAAASPDIVRADMLVEGVAAAGGDDPRQHRLGPPLTDDERCVHA
                     FDDALARLVAGAREAGAAAIVGVVSDYKGEGHAIRDGAQFECHAGGVRSYVWLRAQLV
                     RSLPAARALPPATGFARLDDVDAVPLSDAGKERYRHFLTLPSPRAFVVYEDGNWRFYA
                     RDPEAASKALDYCARQGKRCWLYAADDRVLWSADVGKRIGSAAQLGGDASPGDAKDER
                     R"
     gene            complement(1813..3450)
                     /locus_tag="VIN75_20735"
     CDS             complement(1813..3450)
                     /locus_tag="VIN75_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8976654.1"
                     /translation="MPILSTQLNARSEDFKANAAAMRLLVDDLNAKLAKIALGGGDEA
                     RKKHLSRGKLLPRDRVERLLDPGTPFLEIAPLAALGMYPEKGGQDAAPGAGMIAGIGR
                     VAGVECLIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQANLSAQGIPQLAVVMGSCTAGGAYVPAMSDQTVIVKNQGTIFL
                     GGPPLVKAATGEVVSAEDLGGGDVHTRLSGVADHLAENDMHALALARGIVGTLNWRKD
                     AGVALREPAPPRLPAHELHGIIPLDAEGKPARKPFDVREVIARIVDGSEFEEFKARYG
                     ATLVCGFAWIEGMPVGIVANNGILFAESADKGAHFIELCCQRKVPLVFLQNITGFMVG
                     RKYENEGIARAGAKMVTAVACANVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNA
                     RISVMGGEQAASVLATVRRDGIEARGGQWSAEEEETFKAPIRDQYEAQGHPYFASARL
                     WDDGVIDPADTRRVLALGLSASLNAPIPDMKFGVFRM"
     gene            3702..4184
                     /locus_tag="VIN75_20740"
     CDS             3702..4184
                     /locus_tag="VIN75_20740"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976655.1"
                     /translation="MTGNTTKNLYRSIHKSTPGYDGGPIVDGAAVAGVLYPDFEPRKI
                     SKTRTRAADLTAFDGEGGVKMVRNDGGTSLFDKANVLPGGADTWHSFKLPPETVIPAS
                     LKIRFTGYNKTFDADHWQIESVAGTMAMDAMKGALDNLARNAIVRLIALGEVKAPSKV
                     "
     gene            4220..4453
                     /locus_tag="VIN75_20745"
     CDS             4220..4453
                     /locus_tag="VIN75_20745"
                     /inference="COORDINATES: protein motif:HMM:NF044532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976656.1"
                     /translation="MAEVSGNTLLMAVQAVNDAIAMLDERIRDGSGDPLDDTEMLMSY
                     TRAADELRSAYEVARLTSSNLPPYEDLVASDDA"
     gene            4558..4941
                     /locus_tag="VIN75_20750"
     CDS             4558..4941
                     /locus_tag="VIN75_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8976657.1"
                     /translation="MQFGYTILYVDDVAASLDFYGQAFALPTRFLHDSGSYGELETGA
                     TALAFASRAMLRELGKTPQAPSLGQPTSEIALVTDDVPAAVARAVAAGATLVKPPQDM
                     PWGQTIAYVGDVNGFLVELCTPVKG"
BASE COUNT          741 a         1779 c         1817 g          748 t
ORIGIN      
        1 cgatcacggc gtcgttgaac gcgttgcgca cgtccgggcg gttcaggaag acgcgggcga
       61 cggcaccgtc gcgcgagatc tgcagggtgg cggtcatcgt tgcaggctca gttcgacgcc
      121 ggcgcgggcg ccggcggcaa gggctggaag gtgggcggag gcggcggcgg cacgaggtcg
      181 ctgcgcggct gcggcggcga gatcaccggc acggccggcg acacgggctg cggcggcggc
      241 gacaccaccg gcacctcgga tggcggcgtc ggcgccaccg gcccgaccgc gagctgcgcg
      301 cgcagcgtga cggcggcctt cagcgcgccg gcgtggcatt cgaaatgcgt cgggtcgtcg
      361 aagacgctgc cctcgtagtt cgagacgacg cccacgatgg cgcttgcgcg cgccccgcgg
      421 gcggcgaacg ccagctgcgt gatcgcgtcc tcgaaggcgc gggcgcacag gtcgtggtcg
      481 gtcgggcgca cgcgatcgtc gtcgccgaca tccttgaccc tggtggtgcc cacgcccttc
      541 acgacgacgt cgtggcgcac gatgctggcg ccgcgcgcgg tgtcgctgcc gaagcgcagg
      601 tcgaggttgc ccaggatgtc gtgggtacgg gtgacgcgga tcgtgtccgg caccgattcc
      661 atcaggcgga cgttgcgcgc ctgcgcgggc ggcgcgagca cggacagtgc cgcggcaacg
      721 gcgaggacgg cgggcgtggc ccgcgtcggg aacgacggga tgcgaagctg gttcatggtt
      781 gggccctttc tggcacgacg accgcggacg cggtctgacc ggcgggcagg gcaaatcccc
      841 tgccgtgccc gctgccgccg gcgcgggatg acgcctgctg cgcggtcacc ggcgctcgtc
      901 cttggcgtcg ccgggcgagg cgtcgccccc cagttgcgcg gcgctgccga tgcgcttgcc
      961 gacgtcggcg ctccagagca cgcgatcgtc ggcggcgtag agccagcagc gcttgccctg
     1021 gcgcgcgcag tagtccagcg ccttgctcgc cgcctccgga tccctggcgt agaagcgcca
     1081 gttgccgtcc tcgtagacga cgaacgcgcg cgggctgggc agcgtcagga agtggcgata
     1141 gcgctccttg cccgcgtcgc tgagcggcac cgcgtcgacg tcgtccagcc tggcgaagcc
     1201 ggtggccggc ggcagcgcgc gcgcggccgg caggcttcgc acgagctgcg cgcgcagcca
     1261 gacgtaggag cgcacgccgc cggcatggca ttcgaactgc gcgccgtcgc ggatggcgtg
     1321 gccctcgccc ttgtagtcgg acacgacgcc cacgatggcc gcggcgccgg cctcgcgcgc
     1381 acccgcgacc agcctggcca gcgcgtcgtc gaacgcatgg acgcagcgct cgtcgtcggt
     1441 cagcggcggc cccaggcgat gctggcgcgg gtcgtcgccg ccggcagcgg cgacgccctc
     1501 gaccagcatg tccgcgcgca cgatgtcggg cgacgcggcc gccggcgcgc tcgccgcgcc
     1561 gaagcgcacg ggcatgtcgc ccacgatctc gcgggcgcgg ttcttgtgga acgcgtcgtg
     1621 cacggacagc gtgaacgtct cgttgcgcgc gagtgccggc ctcgccaccg ccagcacggc
     1681 ggcggagatc aacagggaga gcgggacgga acggagcatg tcgggtcgga cggaggggtg
     1741 gatcgcggga tcatcgcttg cgggcggcgg cgccagacga cgcggggctg aacgaagccc
     1801 gacgccgcgc gctcacatgc ggaacacgcc gaacttcatg tccgggatcg gcgcgttcag
     1861 gctcgcggac aggcccagcg cgagcacgcg gcgcgtgtcg gcggggtcga tcacgccgtc
     1921 gtcccacagg cgcgcgctgg cgaagtacgg gtggccctgg gcctcgtact ggtcgcggat
     1981 cggcgccttg aaggtctcct cctcctcggc cgaccactgg ccgcccctgg cctcgatgcc
     2041 gtcgcggcgc acggtggcca gcacgctcgc ggcctgctcg ccgcccatca cgctgatgcg
     2101 ggcgttgggc cacatccaca ggaagcgcgg gctgtaggcg cggccgcaca tgccgtagtt
     2161 gccggcgccg aacgagccgc cgatgatgac cgtgaacttc gggacgttgg cgcaagccac
     2221 ggccgtcacc atcttggcgc cggcgcgcgc gatgccttcg ttctcgtact tgcggcccac
     2281 catgaagccg gtgatgttct gcaggaacac gagcgggacc ttgcgctggc agcacagctc
     2341 gatgaagtgc gcccccttgt cggcgctctc ggcgaacagg atgccgttgt tggcgacgat
     2401 gcccacgggc atgccctcga tccacgcgaa gccacagacc agcgtcgcgc cgtagcgcgc
     2461 cttgaactcc tcgaactccg agccatcgac gatgcgggcg atgacctcgc gcacgtcgaa
     2521 gggcttgcgc gcgggcttgc cttcggcatc gagcgggatg atgccgtgca gctcgtgcgc
     2581 gggcagcctg ggcggcgcgg gttcgcgcag cgcgacgccg gcgtccttgc gccagttgag
     2641 cgtgcccacg atgccgcgcg ccagcgccag cgcgtgcatg tcgttctcgg ccaggtggtc
     2701 ggccacgccc gacaggcgcg tgtgcacgtc gccgccgccc aggtcctccg cgctgaccac
     2761 ctcgccggtg gccgccttca ccagcggcgg cccgcccagg aagatggtgc cctggttctt
     2821 gacgatgacc gtctggtcgc tcatggccgg cacgtaggcg ccgccggcgg tgcacgagcc
     2881 catcaccacg gccagctgcg ggatgccctg cgcgctgagg ttggcctggt tgtagaagat
     2941 gcggccgaag tggtcacggt ccgggaacac ctcgtcctgg ttgggcaggt tggcgccgcc
     3001 ggagtcgacg aggtagatgc acggcaggcg gttctgctgc gcgatctcct gcgcccgcag
     3061 gtgcttcttg accgtcatcg ggtagtaggt gccgcccttg acggtggcgt cgttgcacac
     3121 gatcaggcac tcgacgcccg ccacgcggcc gatgcccgcg atcatgccgg cgcccggcgc
     3181 cgcgtcctgg ccgccctttt ccgggtacat gcccagcgcg gccagtggcg cgatctccag
     3241 gaacggcgtg cccgggtcca gcaggcgctc gacgcgatcg cgcggcagca gcttgccgcg
     3301 cgacaggtgc ttcttgcgcg cctcgtcgcc gccacccagc gcgatcttcg cgagcttggc
     3361 gttcaggtcg tcgaccagca ggcgcatcgc cgcggcgttg gccttgaagt cttccgagcg
     3421 ggcattcagt tgggtcgaga ggatgggcat gggttcctgg cttcttgtct cggtggtccc
     3481 gcccgggcgc gcgtggccgc gcgggcggga tcgccatctt ggccgaggat tatcgattga
     3541 cgtttacgta aacgtcaatt tcaattcggc ggacgccgcc ggcgcgatgc aggaacccct
     3601 tgagcgaggg ctggcagccc gcatggggtc ggccaagaat ggaccgatgc ggcccgcccc
     3661 tccatgcggc cgtcactcca gggagcgcct ccatgaccga aatgaccggc aacaccacca
     3721 agaacctcta tcgttccatt cacaagagca cacccggcta tgacggcggc ccgatcgtcg
     3781 acggcgccgc cgtggccggc gtgctctatc cggacttcga gccccgcaag atcagcaaga
     3841 cccgcacgcg cgctgccgac ctcaccgcct tcgacggcga gggcggcgtg aagatggtgc
     3901 gcaacgacgg tggaacgtcg ctgttcgaca aggccaacgt cctccccggc ggcgccgata
     3961 cctggcacag cttcaagctg ccgccggaga ccgtcatccc ggcgtcgctg aagatccgct
     4021 tcaccggtta caacaagacc ttcgatgccg accactggca gatcgagagc gtcgccggca
     4081 cgatggcgat ggacgcgatg aagggcgcgc tcgacaatct ggcccgcaac gccatcgtcc
     4141 gcctgatcgc gctcggcgaa gtcaaggcgc ccagcaaggt ctgagttcca gatgcgtacc
     4201 acttccaacg agactttcca tggctgaagt cagcggcaac acccttctca tggccgtgca
     4261 ggccgtcaat gatgcgattg caatgctcga cgagcgcatc cgcgacggct cgggcgaccc
     4321 gctcgacgac acggagatgc tcatgagcta cacccgggcc gccgatgaac tgcgctcggc
     4381 ctacgaagtg gcccggctga cctccagcaa cctgccgccc tacgaggacc tggtcgcgtc
     4441 ggacgacgcg tgacgcaggc cgcgacgtcg gaattcttca agacacgcgg ccttcctccc
     4501 catatcgttg ctgccagcca gccgcccgac ccgagtggcg acgaaacaag gaacggcatg
     4561 caattcggct acaccatcct ctacgtcgat gacgtcgcgg catccctcga cttctacggc
     4621 caggccttcg ccctgcccac ccgcttcctg cacgacagcg gcagctacgg tgagctggag
     4681 accggcgcca ccgcgctggc cttcgcgtcg cgagcgatgc tgcgcgagct tggcaagacg
     4741 ccgcaggcgc catcgctcgg ccagcccacc agcgagattg cgctcgtcac cgacgatgtg
     4801 ccggccgcgg tggcgcgcgc cgtggcggcc ggtgccacgc tcgtgaagcc accccaggac
     4861 atgccctggg gccagacgat cgcctacgtg ggcgacgtca acggcttcct ggtcgagctg
     4921 tgcacgccgg tgaagggttg aggcctgggc cgcctggcgg cttggcgacg acgaggggtc
     4981 cggcttggcc tctcgagcat cgcgatggcg gagagacgtg gccgaggggt ctggctccgg
     5041 cctctcgcgc gccgcgatag ggcaatggcg aggccggagc ctgac
//
LOCUS       DATCRO010000763         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_121140, whole genome shotgun sequence.
ACCESSION   DATCRO010000763 DATCRO010000000
VERSION     DATCRO010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121140"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIN75_20755"
     CDS             complement(<1..432)
                     /locus_tag="VIN75_20755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8976658.1"
                     /translation="MTGEQIRFDDGAAYEDFMGRWSLLAGNAFLDWLSPAKGLRWLDV
                     GCGNGAFTELLVERCAPRSVQGIDPSEAQVAFARTRLAGAPGITLRTGSAMALPYEDG
                     AFDAVASALVIFFVPDAPGAVVEMARVTRPGGGVCAYAWDLL"
     gene            594..2369
                     /locus_tag="VIN75_20760"
     CDS             594..2369
                     /locus_tag="VIN75_20760"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY8976659.1"
                     /translation="MTLHQAEAFIVIAAMLALFMSGRLRFDLVAALALVAAVVLGVVP
                     AGKAFSGFSNPVIIIIGSVLVVSRAIAASNVLDVWIGALLARVKSPSLQIGILTAAVG
                     YLSAFVKNVGTLGIVMPMAIQAARRSNMPRSAYLMPLAFGSLVGGTITKIGTSPNLLI
                     SSVRQEIGLPGFRLFDFVPVGLPLTTLAIAFLAVGWRLIPRREGQKSAEESFSIESYS
                     TELRVPATPALPELTVRSLEELSEGEASVAAIVREGGRRYIPAEDWRIYAGDVLVVLG
                     DPTVIDKLVAKTGYEIAGSKELAQPEHKTDEMEVLEVIVPRDSMLVGQTPASLNLRHR
                     FGVNLFAVHQASKATVRRLRSHCFSAGDVVLLQGWGKKLQASVDELGVLPLADRKLTL
                     GRKASGLIPIALLAVALLLVGLRWVDVEVAFFGAAALTVLARQINAKEAYDAVEWPVI
                     VMLGCLIPVGEALKDTGATALIAHGLTHAAGQVPGYAAVGLVVLTAMLLTPLLHHAAA
                     VLVLGPVAAAVATNLGYRADPFLMAVALGCACDFLTPIGHQNNTLVMGPGGYRFGDYW
                     RLGLPLTLMVLFVGTPLILHAWPLK"
     gene            complement(2401..3567)
                     /locus_tag="VIN75_20765"
     CDS             complement(2401..3567)
                     /locus_tag="VIN75_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8976660.1"
                     /translation="MKHLTAFLLSCLLSANALADITALVVTEPSNRKDAMMVSREALQ
                     AHLSRVLKDAVVVTTTDDMTDAMRATRSGGYDVFIAPPQVAASALGHGYALVGATDSD
                     EQYLLVGRSDLASVGALRGKEIYLPQQDSIYTYLARGLLNANGLSFKDMKHIDYERYP
                     QAGLYALMLGRSEATMVRSADWESWDREHAGIAKVLAISSAVPGGYSVTVRRKLPSET
                     RGKLAHWFENDGVACGLKPVFAHPDLTPYTRVAELGTFTPKALPGATVVDHDAVKALV
                     SNGAVLVDTRIESEYKDKHIPGALWLPYGEKSLKDVAFDAKVDNFPGLARLDRAQNIV
                     FQCNGPECWKSYKASRAAIAAGYKHIYWFRGGMPEWEMAGERTEKTGATLALSN"
     gene            3845..5068
                     /locus_tag="VIN75_20770"
     CDS             3845..5068
                     /locus_tag="VIN75_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8976661.1"
                     /translation="MTNPPANRLRRRLVVAGGAGLATSLAGRAARAAGAGVELPIVNG
                     HRDLVAFPEKRPLIVITSRPPQLETPFSVFNEGVITPNDAFFVRYHNAGIPTSIDGDR
                     HVIKVGGNAAGKSFELTMADLRTQFKPVELVAVNQCSGNGRALFNPRVTGGQSTNGTM
                     GNARWVGVPLKDILARAEVKNTARQVTFDGLDMALMGGGDFVKALDINVATNGEVMVA
                     YQMNGAELPFLNGYPVRLVVPGYYGTYWVKHLSDIQVIDQVYDGFWMKPAYRVPDNAC
                     ACVEPGTAPAATRPIGRFNVRSFITSLADGARVPAGRALAVRGIAFDGGQGIREVAWS
                     ADGGQSWREATLGEDLGRFSFREFTFGFTPAAGAHDLRVRAFNRSGESQPMEALWQPS
                     GYMRNVVESVKVQAS"
     gene            5079..5402
                     /locus_tag="VIN75_20775"
     CDS             5079..5402
                     /locus_tag="VIN75_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8976662.1"
                     /translation="MKHATLLAVACVAAGLALPLAAKDIQLPPDAVQLKPSPLPGYAK
                     AQANCTMCHSAEYMRMQPPTAARPYWDAMVHRMKTVFKAPVADEDMPEIVDYLARTYG
                     SEQPK"
BASE COUNT          810 a         1938 c         1935 g          875 t
ORIGIN      
        1 gagcaggtcc caggcgtagg cgcacacgcc gccgccgggg cgcgtgacgc gcgccatctc
       61 gacgaccgcc ccgggcgcgt cgggcacgaa gaagatcacc agcgccgagg cgacggcgtc
      121 gaacgcgcca tcctcgtacg gaagcgccat cgcgctgccg gtgcgcagcg tgatgccggg
      181 cgcgcccgcg aggcgcgtgc gcgcgaacgc gacctgcgcc tcggacgggt cgatgccctg
      241 caccgacctg ggcgcgcagc gttcgaccag cagctcggtg aacgcgccgt tgccgcatcc
      301 cacgtccagc cagcgcaggc ccttggcggg cgacagccag tccaggaagg cgttgccggc
      361 cagcaggctc cagcggccca tgaagtcctc gtaggccgca ccgtcgtcga agcggatctg
      421 ttcgccggtc atcgtcgttc tcccgtggat cggatgagtc tggcgagtat cgcgcgggcg
      481 tgcccggaaa gacacgtctt ccacgagggt gctgccgtcg agcatggcac cccgtttgcc
      541 atgccccgcc cgaggagggc cgctgccgtg gcgtgcccga tgccggagca cgcatgaccc
      601 tgcaccaggc cgaggccttc atcgtcatcg ccgccatgct cgccctgttc atgagcgggc
      661 ggctgcggtt cgacctcgtc gccgcgctcg cgctggtggc ggccgtcgtg ctgggcgtgg
      721 tgccggcggg caaggcgttc tccggcttct ccaacccggt catcatcatc atcggctcgg
      781 tgctggtggt cagccgcgcg atcgcggcgt ccaacgtgct ggacgtgtgg atcggagcgc
      841 tgctcgcacg cgtgaagtcg ccgtcgctgc agatcggcat cctcacggcc gcggtcggct
      901 acctgtcggc cttcgtcaag aacgtgggca cgctgggcat cgtgatgccg atggcgatcc
      961 aggcggcacg gcgctcgaac atgccgcgct cggcctacct gatgccgctg gccttcggct
     1021 cgctggtggg cgggaccatc accaagatcg gcacgtcgcc caacctgctg atctccagcg
     1081 tgcgccagga gatcggcctg ccgggcttcc ggttgttcga cttcgtgccg gtgggcctgc
     1141 cgctgaccac gctggccatc gcgttcctgg cggtgggctg gcgcctgatc ccccggcgcg
     1201 aaggccagaa gtcggccgag gagagcttct cgatcgagtc ctattccacc gagctgcgcg
     1261 tgcccgcgac accggcgctg cccgagctga cggtcaggtc gctcgaggag ctctcggaag
     1321 gcgaggcgtc ggtcgcggcc atcgtgcgcg agggcggccg gcgctacatc ccggccgagg
     1381 actggcgcat ctacgccggc gacgtgctgg tggtgctggg cgacccgacc gtcatcgaca
     1441 agctggtcgc caagacgggc tacgagatcg ccggctccaa ggaactcgcg cagcccgagc
     1501 acaagaccga cgagatggag gtgctcgagg tcatcgtgcc gcgcgactcg atgctggtgg
     1561 gccagacgcc tgcgtcgctg aacctgcgcc atcgcttcgg cgtcaacctg ttcgcggtgc
     1621 accaggcgag caaggccacg gtcaggcgcc tgcgctcgca ctgcttttcc gcgggcgacg
     1681 tcgtgctgct gcagggctgg ggcaagaagc tgcaggcgtc ggtggacgag ctcggcgtgt
     1741 tgccgctggc cgaccgcaag ctcacgctcg ggcgcaaggc gagcggcctg atcccgatcg
     1801 cgctgctggc cgtggcgctg ctgctggtgg gcctgcgctg ggtcgacgtc gaggtggcct
     1861 tcttcggcgc cgcggcgctg accgtgctgg cgcgccagat caacgccaag gaggcctacg
     1921 acgcggtcga atggccggtg atcgtgatgc tgggctgcct gatcccggtc ggcgaggcgc
     1981 tcaaggacac gggcgccacg gcgctgatcg cgcacggcct gacgcacgcc gccggccagg
     2041 tgccgggcta cgccgccgtc gggctggtgg tgctcacggc gatgctgctg acgccgctgc
     2101 tgcaccacgc cgcagcggtg ctcgtgctgg ggccggtcgc ggcggcggtg gcgaccaacc
     2161 tgggctaccg cgccgacccg ttcctgatgg cggtggcgct cggctgcgcc tgcgacttcc
     2221 tcacgcccat cggccaccag aacaacacgc tggtgatggg cccgggcggc taccggttcg
     2281 gcgactactg gcgcctcggc ctgccgctga cgctgatggt gctgttcgtg ggcacgccgc
     2341 tcatcctgca tgcgtggccg ttgaagtgag cgccggcccg cgcgtcggcg cgcgagggcg
     2401 tcagttggac agcgccagcg tcgcgcccgt cttctccgtg cgctcgccgg ccatctccca
     2461 ttcgggcatg ccgccgcgga accagtagat gtgcttgtag ccggcggcga tcgcggcgcg
     2521 gctggccttg tacgacttcc agcattccgg cccgttgcac tggaacacga tgttctgcgc
     2581 gcgatccagc ctggccaggc cggggaagtt gtcgaccttg gcgtcgaacg ccacgtcctt
     2641 caggctcttc tcgccatagg gcagccacag cgcgccgggg atgtgcttgt ccttgtattc
     2701 ggactcgatg cgcgtgtcga ccagcaccgc gccgttgctg accagcgcct tgacggcatc
     2761 gtggtcgacc accgtggcgc ccggcagcgc cttgggggtg aaggtgccga gttcggcgac
     2821 gcgcgtgtac ggcgtcaggt ccgggtgcgc gaacaccggc ttcaggccgc aggcgacgcc
     2881 gtcgttctcg aaccagtgcg ccagcttgcc gcgcgtctcg gatggcagct tgcggcgcac
     2941 ggtcaccgag tagccgcccg gcaccgcgct ggagatggcc aggaccttgg cgatgcccgc
     3001 gtgctcgcgg tcccagctct cccagtcggc gctgcgcacc atcgtcgcct ccgagcggcc
     3061 cagcatcagc gcgtacaggc ccgcctgcgg gtagcgttca tagtcgatgt gcttcatgtc
     3121 cttgaagctc aggccgttcg cgttcagcag gccgcgcgcg aggtaggtgt agatcgagtc
     3181 ctgctgcggc aggtagattt ccttgccgcg cagcgcaccg accgatgcca ggtcgctgcg
     3241 gcccaccagc aggtactgtt cgtcgctgtc ggtggcgccc accagcgcgt agccgtgccc
     3301 cagcgcggag gccgcgacct gcggcggggc gatgaagacg tcgtagccgc cgctgcgcgt
     3361 ggcgcgcatc gcgtcggtca tgtcgtcggt ggtagtcacg acgaccgcgt ccttcaatac
     3421 cctggacagg tgagcctgca acgcctcgcg cgacaccatc atggcgtcct tgcgattgct
     3481 gggttcggtg acgacgagag cggtgatgtc ggccagcgcg ttggcactga gcaggcaaga
     3541 cagcaggaaa gcagtgagat gcttcacgat tttcgacctc ctggcggcga tgacggaacc
     3601 ggattccgcg cgctgcgaag acgctctcgg gcacttggcc ggggtcttct tccctcactg
     3661 catggcgctc gaacagggta ggtcagttgc atacggcgtg ctgtatccag acgaaacctc
     3721 gtttggacaa acgcaccccg ggtctacact cccgtcgagc tgcattcgtc catttcgcgg
     3781 atcggtcgag gtccgcggaa tgaaagtcct gtatcggaac cgtcgccatc ccaaggaagc
     3841 tccaatgacg aaccctcccg ccaaccgcct gcgccgcagg ctcgtcgtcg ccggcggtgc
     3901 cggcctggcc acctcgctgg ccggtcgcgc cgcgcgcgcc gcgggcgccg gcgtcgaact
     3961 gccgatcgtc aacggccacc gcgacctggt ggcgttcccc gagaagcgcc cgctgatcgt
     4021 gatcacgtcg cggccgccgc agctcgagac gccgttctcc gtcttcaacg aaggcgtcat
     4081 cacgcccaac gacgcgttct tcgtgcggta ccacaatgcc ggcatcccga cctcgatcga
     4141 tggcgacagg cacgtcatca aggtcggcgg caacgcggcg ggcaagtcgt tcgagctcac
     4201 catggccgac ctgcgcacgc agttcaagcc ggtggagctc gtggccgtca accagtgctc
     4261 gggcaacggc cgcgcgctgt tcaacccgcg cgtgaccggc ggacagagca ccaacggcac
     4321 gatgggcaac gcgcgctggg tgggcgtccc gctcaaggac atcctcgcgc gcgccgaggt
     4381 gaagaacacc gccagacagg tcaccttcga cgggctggac atggcgctga tgggcggcgg
     4441 cgacttcgtc aaggcgctgg acatcaacgt cgccaccaat ggcgaggtga tggtggcgta
     4501 ccagatgaac ggcgccgagc tgccgttcct caacggctat ccggtgcggc tggtggtgcc
     4561 cggctactac ggcacctact gggtcaagca cctgtccgac atccaggtga tcgaccaggt
     4621 ctacgacggc ttctggatga agccggccta ccgcgtgccg gacaacgcct gtgcatgcgt
     4681 cgagcccggc accgcgccgg cggccacgcg cccgatcggc cgcttcaacg tgcgctcgtt
     4741 catcacctcg ctggccgacg gcgcgcgcgt gccggcgggc agggcgctgg cggtgcgcgg
     4801 catcgccttc gacggcggcc agggcatccg cgaggtcgcc tggagcgccg acggcggcca
     4861 gagctggcgc gaggcgacgc tgggcgagga tctcggacgc ttctcgttcc gcgagttcac
     4921 gttcggcttc acgccggcgg ccggcgcgca cgacctgcgc gtgcgcgcct tcaaccgctc
     4981 gggcgagagc cagccgatgg aggcgttgtg gcagccatcg ggctacatgc gcaacgtcgt
     5041 cgagtcggtc aaggtccagg ccagctgaag gaggccccat gaagcacgca accctcctcg
     5101 ccgtcgcctg cgtcgccgcg ggtctggccc tgccgctggc ggccaaggac atccagctgc
     5161 cgcccgacgc cgtgcagctc aagcccagcc cgctgcccgg ctacgccaag gcgcaggcca
     5221 actgcacgat gtgccattcg gccgaataca tgcgcatgca gccgccgacc gccgcgcgcc
     5281 cgtactggga cgcgatggtg catcgcatga agaccgtctt caaggcgccg gtcgccgacg
     5341 aggacatgcc cgagatcgtc gactacctgg ccaggacgta cggcagcgag cagcccaagt
     5401 gagccggcac gccggccagg gcctgttcac actaaatgcg tgcccgcgtg acctccagaa
     5461 agcccgcctt caaggcgcgg gcgcgccgac gtggcggcgc cacggcaagc gctcgcaacg
     5521 ccgaaggcgg gctttctgga ggatcacccg cagggccg
//
LOCUS       DATCRO010000764         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_211341, whole genome shotgun sequence.
ACCESSION   DATCRO010000764 DATCRO010000000
VERSION     DATCRO010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211341"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..386
                     /locus_tag="VIN75_20780"
                     /pseudo
     CDS             1..386
                     /locus_tag="VIN75_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            534..1070
                     /locus_tag="VIN75_20785"
     CDS             534..1070
                     /locus_tag="VIN75_20785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8976663.1"
                     /translation="MPYNVDPAKLRFTPHAFEALRLWRDAGYRLVVVTNQSGIARGLF
                     DRAALERLHDALVERLSQEGVAIDGFFACPHRDEAGCACRKPRAGLLEEAARALGIDL
                     AASWMVGDILNDVEAGHRAGCRSVLLDVGHESEWVPGAMRSPEHRCANLLEAAHAILE
                     STVPVAMAAEGDALRVAA"
     gene            1129..2271
                     /locus_tag="VIN75_20790"
     CDS             1129..2271
                     /locus_tag="VIN75_20790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8976664.1"
                     /translation="MTAPAMAADAARRAAWRGVRRLLAVRVDNLGDVLMTTPALAAAA
                     ETLPGVEITLLGGPGAGALAPHLPMVREIVPARVPWVPGEADPRNDHVLVERLADGRF
                     DAAVIFTVCTQSALPAALLCRLAGIPLRLAHVRENPYGLLSDWVPETDLVLDDARHEV
                     RRQLDLVGHVGWTTPDERLRFRLRECDIARIDAWLDGEAGDGDDRPLVVVHVGATAPS
                     RRWPAERFGQAADVLAYEQGARIVFTGSADETALVAAARACMKQPSHSLAGRLTLGQL
                     GALIARADLLLCNNSGPAHLAAALGTPVVDVYALTNPQHTPWQVPARVLSRDVDCRGC
                     LKSRCPQGHHRCIADVAPAEAVDAALSLLGQPRAETFQPLLLETLA"
     gene            2268..4010
                     /locus_tag="VIN75_20795"
     CDS             2268..4010
                     /locus_tag="VIN75_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8976665.1"
                     /translation="MITLGINAAFHDSAAALVRDGVLVAAAEEERFSRIKHGKRPVPF
                     SAWELPFAAIDYCLREAGITLADVDHVAYSYDPARFVRDRLPNGFALLPMDLDSVERS
                     DWDSPWDPLFACHLVNAPRQLADGAPHHLQARFRGVRHDGPFRFHYLDHHLCHQASAF
                     LAAPFERCAVMTLDGRGEEATTAYGRWRDGRYEALGEVTMPHSLGLLYEKITSHLGFL
                     HSSDEYKVMALAALGKPTMVGRFLEMIEIGANGRYTIADFDPVALFGPARERGAPLEQ
                     HHFDLTASLQLALERTVLRLASWLRDTSGERHLAMAGGVALNCVMNAKLRDAGIFDEV
                     WVQPAAGDAGTALGAALWTDHRERSTLAGAPVARTWTMKHAYWGPGFDDDEIEQLLRW
                     AKLRYRRLDDVAGETARLLAQDHIIGWFQGRMEFGPRALGGRSILASPCSPQMQARLN
                     ELKDREDFRPVAPVLPREDLSAWFMPAEANGGEAPFMLFLYDVRPGQAARIPAVCHAD
                     RTARVQTVERGTNPRYHDTLKAFQAITGVPVLVNTSFNVRGEPVVCTPKDALDAFFST
                     PLDALVIGRFLLEK"
     gene            4039..>4485
                     /locus_tag="VIN75_20800"
     CDS             4039..>4485
                     /locus_tag="VIN75_20800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8976666.1"
                     /translation="MDFPIPSLSVVIPTYRRPALLARCLDALLAQSLPGHAFEVIVVD
                     DGCTEDTRACCSEVAIRSCRRGGPVIRYLRPQGTRGPAAARNRGWRAAQGRVVAFTDD
                     DTVPDPDWLRQGQTAFATNGVEAAWGRVHVPLPPAMTDNARNTAGLE"
BASE COUNT          577 a         1657 c         1643 g          608 t
ORIGIN      
        1 tgcaggcgct ggcgcgcgag gagggcatcg gcggctcggt ggtgttcacc gggcggcgca
       61 gccgcgagtt cctcaagctc tattacagcg ccgcggacat cttcgtcacc acgccctggt
      121 acgagccgtt cggcatcacg ccgctcgagg cgatggcctg cgcgcggccc gtcgtcggca
      181 gcgcggtcgg cggcgtgcgc tacaccgtgg tcgacggcat caccggcctg ctggtgccgc
      241 cgaaggacga acgcgcgctc gcccacgccc tcgcgcgcct gcgcgccgaa cctgcgcgcg
      301 cacgggcgat gggcgaggcc ggcctgcggc gcgtgcgcgc cgagttcacg tgggcgcggg
      361 tcgcggcgag cctggccggc gtcttcgacg acgcggtgcg cgcgcgcgcc cggcgcggcc
      421 acgcctcgat cttccacctg gcgggcctcg acgccgcgcg ccccgcgaca gtgccgggca
      481 gcgcgcgccg gccggcgatc ttcctggaca aggacggcac gctgatcgag gacgtgccgt
      541 acaacgtcga tcccgcgaag ctgcgcttca cgccgcacgc gttcgaggcg ctgcggctgt
      601 ggcgtgacgc cggctaccgg ctcgtcgtcg tcaccaacca gtcgggcatc gcccgcggcc
      661 tgttcgaccg cgctgcgctc gagcggctgc acgatgcgct ggtcgaacgg ctgtcgcaag
      721 agggcgtcgc gatcgacggc ttcttcgcct gcccgcatcg cgacgaggcc ggctgcgcct
      781 gccgcaagcc gcgcgcgggc ctgctggagg aggcggcgcg cgcgctgggc atcgacctgg
      841 cggcgtcctg gatggtgggc gacatcctca acgacgtgga ggccggccac cgcgccggct
      901 gccgcagcgt cctgctggac gtcggccatg agtcggagtg ggtgccgggc gcgatgcggt
      961 cgccggagca tcgctgcgcg aacctgctgg aggccgcgca cgcgatcctc gagtcgacgg
     1021 tgcccgtcgc gatggcggcc gaaggcgacg cgctgcgggt ggctgcatga gtccgcacgg
     1081 ccgaccgaag ggcgaatcga ccgcagtccg caggacggag gctgccgcat gaccgcgccg
     1141 gcgatggcgg ccgacgcggc gcggcgggcc gcgtggcgcg gcgtgcggcg cctgctggcc
     1201 gtgcgcgtcg acaacctcgg cgacgtgctg atgacgacgc ccgcgctcgc ggccgcggcc
     1261 gagacgctgc ccggcgtcga gatcacgctc ctgggcggcc ccggcgcggg tgcgctcgcc
     1321 ccgcacctgc ccatggtgcg cgagatcgtt cccgcgcgcg tgccctgggt gcccggcgag
     1381 gccgacccgc gcaacgacca tgtcctcgtc gagcgcctgg ccgacggccg tttcgacgcc
     1441 gccgtcatct tcacggtgtg cacgcagagc gcgctgccgg ccgcgctgct gtgccgcctg
     1501 gccggcatcc cgctgcgcct cgcgcacgtg cgcgagaacc cgtacggcct gctcagcgac
     1561 tgggtgcccg agacggacct cgtgctggac gacgcgcgcc acgaggtgcg gcgccagctc
     1621 gacctggtcg gccacgtcgg ctggacgacc cccgacgaac gcctgcgctt tcgcctgcgc
     1681 gagtgcgaca tcgcgcgcat cgacgcctgg ctggacggcg aggccgggga cggcgacgac
     1741 cggccgctgg tggtcgtgca cgtcggcgcg acggcgccgt cgcgccgctg gcccgcggag
     1801 cgcttcggcc aggccgccga cgtgctcgcg tacgagcagg gcgcacgcat cgtgttcacg
     1861 ggcagcgccg acgagaccgc gctggtggca gcggcgcgcg cctgcatgaa gcagccgtcg
     1921 cattcgctgg cggggcggct gacgctgggc cagctcggcg cgctcatcgc ccgcgccgac
     1981 ctgctcctgt gcaacaacag cggcccggcg cacctggccg cggcgctcgg gacgccggtg
     2041 gtcgacgtct acgcgctgac gaacccgcag cacacgccgt ggcaggtgcc cgcgcgcgtg
     2101 ctcagccgcg acgtcgattg ccgcggctgc ctcaagagcc gctgcccgca ggggcaccac
     2161 cgctgcatcg ccgacgtcgc gcccgccgag gcggtcgacg cggcgctgtc gctgctgggc
     2221 cagccgcgtg ccgaaacctt ccaacccctc ttgctggaga ccctcgcatg atcaccctgg
     2281 gcatcaacgc cgccttccac gacagcgccg ccgcgttggt gcgcgacggc gtgctcgtcg
     2341 ccgccgcgga ggaagagcgc ttctcgcgca tcaagcacgg caagcggccg gtgccgttct
     2401 cggcctggga gctgccgttc gccgcgatcg actactgcct gcgcgaggcg ggcatcacgc
     2461 tggccgacgt cgaccacgtg gcctacagct acgacccggc gcgcttcgtg cgcgaccgcc
     2521 tgcccaacgg cttcgcgctg ctgccgatgg acctggacag cgtcgaacgc tcggactggg
     2581 acagcccgtg ggatccgctg ttcgcgtgcc acctggtgaa cgcgccacgc cagctggccg
     2641 acggcgcgcc gcatcatctg caggcgcgct ttcgcggcgt gcgtcatgac ggcccgttca
     2701 ggttccacta tctcgaccac cacctgtgcc accaggccag cgccttcctg gccgcgccgt
     2761 tcgagcgctg cgcggtcatg acgctggacg gccgcggcga ggaggcgacg accgcgtacg
     2821 gccgctggcg cgacgggcgc tacgaggcgc tcggcgaggt gacgatgccg cattcgctgg
     2881 gcctgctgta cgagaagatc acttcgcacc tgggcttcct gcattcgagc gacgagtaca
     2941 aggtgatggc gctggccgcg ctcggcaagc cgacgatggt gggccgcttc ctggagatga
     3001 tcgagatcgg tgcgaacggc cggtacacca tcgcggactt cgaccccgtc gcgctgttcg
     3061 ggcccgcgcg cgaacgcggc gcgccgctcg agcagcatca cttcgacctc accgcctcgc
     3121 tgcagctggc gctggagcgc acggtgctgc gcctggcctc gtggctgcgc gacaccagcg
     3181 gcgagcgcca cctcgcgatg gccggcggcg tcgcgctgaa ctgcgtgatg aatgccaagc
     3241 tgcgcgacgc cggcatcttc gacgaggtct gggtgcagcc cgcggccggc gatgccggca
     3301 ccgcgctcgg cgcggcgctg tggacggatc accgcgagcg cagcacgctg gccggcgcgc
     3361 cggtcgcgcg cacctggacg atgaagcatg cgtactgggg gccgggcttc gacgacgacg
     3421 agatcgagca gctgctgcgc tgggccaagc tgcgctatcg ccggctggac gacgtggccg
     3481 gcgagacggc caggctgctg gcgcaggatc acatcatcgg ctggttccag ggccgcatgg
     3541 aattcggccc gcgcgcgctg ggtgggcgtt cgatcctcgc ctcgccgtgc agcccgcaga
     3601 tgcaggcgcg cctgaacgag ctgaaggacc gcgaggactt ccggcccgtc gcgccggtgc
     3661 taccgcgcga ggacctgtcc gcctggttca tgcccgccga ggccaacggc ggcgaggcgc
     3721 cgttcatgct gttcctgtac gacgtgcgcc ccggccaggc cgcgcgcatc cctgcggtct
     3781 gccacgccga ccgcaccgcg cgcgtgcaga ccgtggagcg cggcaccaac ccgcgctacc
     3841 acgacacgct caaggccttc caggccatca cgggcgtgcc cgtcctcgtc aacacctcgt
     3901 tcaacgtgcg cggcgagccg gtggtgtgca cgcccaagga cgcgctcgac gcgttcttct
     3961 cgaccccgct cgacgccttg gtcatcggcc gcttcctgct ggagaaatag cggcatgtcg
     4021 acaagtgaga agagaacgat ggacttcccg atcccgtccc tgagcgtggt gatcccgacg
     4081 taccgccgcc cggcgctgct cgcgcgctgc ctggacgcgc tgctggcgca gtcgctgccg
     4141 ggccatgcgt tcgaggtgat cgtcgtcgac gacggctgca ccgaggacac gcgcgcctgc
     4201 tgcagcgagg tggccatccg ctcatgccgg cgcggcgggc cggtgatccg ctacctgcgg
     4261 ccgcagggca cgcgcgggcc ggccgcggcg cgcaaccgcg gctggcgcgc ggcacagggc
     4321 agggtggtcg cattcaccga cgacgacacc gttcccgatc cggactggct gcgccagggg
     4381 cagacggcgt tcgcgacgaa cggcgtcgag gccgcgtggg gccgagtgca cgtgccgctg
     4441 ccgccggcga tgaccgacaa cgcgcgcaac accgcgggcc tggag
//
LOCUS       DATCRO010000765         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_661386, whole genome shotgun sequence.
ACCESSION   DATCRO010000765 DATCRO010000000
VERSION     DATCRO010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661386"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VIN75_20805"
     CDS             <1..1546
                     /locus_tag="VIN75_20805"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8976667.1"
                     /translation="RLSLPELQKLAEGGNGMAQFYLARRYATGDGAEHDDVQAFTWAT
                     KSAAQDLPEGQDALGIFYSYGTGVARDPAKACDWYEKAAHQGHAHAQLGLALCFDQGD
                     GGRPKDASQAFAWTRKAAEQGYGPAQNRLGLYYENGQGVDANLALALAWYRRSGDGGH
                     MRGLYNAARLTEQGRGTPVDLASAAADYRKAADGGEAYAANNLALMYQYGRGVPKDVA
                     QATVLLRQAAGMGLAMAQNNLGHLLSGNKSASDDAEAFGWFTKAAEQGDIQARYALGQ
                     CYSQGRGVSADKRAAARWYREAAEQGVREAQFSLGYLLELGDGVPLDRAQAFDWYRKA
                     ADQGLPEAQYNLASLAERGGSPVPLRPEEIVGWYRKAADNGLPLAQNNLGRHYATGTG
                     VPRDFAMAVTLFRQAAQQGVAEAQNNLGLMLAMGKGVPRDEHAAVEWLRRSAAQDYDL
                     AQMNLANAYYEGRGVAVDRALACQWIRRAEATHRRTATPALPTAQAGANDQMLAKSLA
                     RDCAGR"
     gene            complement(1556..>2103)
                     /locus_tag="VIN75_20810"
     CDS             complement(1556..>2103)
                     /locus_tag="VIN75_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8976668.1"
                     /translation="PKPNRGWIDRIKKPYSGLGPGWKDIAIAELPQVYRAMFPPHKRH
                     RHVGESFGPSGAWWLRSRVENRIPTVFGSTLVDARMAGGKVALTVRQDGDEQELRADR
                     VIAATGFRIDLDRIDYLDPALSASIEREGAAPRLDRHYQTSVPGLHIVGAASAPTFGP
                     VMRFMFGAKHAAPTLARSLRN"
BASE COUNT          321 a          756 c          771 g          255 t
ORIGIN      
        1 ccgcctgtcg ctgccggagc tgcagaaact ggccgagggc ggcaacggca tggcgcagtt
       61 ctacctggcg cgccgctacg cgacgggcga cggcgcggag cacgacgacg tgcaggcgtt
      121 cacctgggcc accaagtccg ccgcgcagga cctgcccgag ggacaggacg cactgggcat
      181 tttctattcg tacggcacgg gcgtggcgcg cgacccggcc aaggcgtgcg actggtacga
      241 gaaggccgcg caccagggcc atgcgcacgc gcagctcggc ctcgcgctgt gcttcgacca
      301 aggcgatggc ggccgaccca aggacgcgtc ccaggcattt gcctggacgc gcaaggcggc
      361 cgaacaaggc tatgggccgg cgcagaaccg gctgggcctg tactacgaga acggccaggg
      421 cgtggacgcc aacctggcgc tcgccctcgc gtggtaccgc cgctccggcg acgggggcca
      481 catgcgcggc ctgtacaacg ccgcgcggct gaccgagcag ggccgcggca cgccggtcga
      541 cctggcgtcc gcggcagccg actaccgcaa ggccgccgat ggcggcgagg cgtacgcggc
      601 caacaacctc gcgctgatgt accagtacgg ccgcggggtg cccaaagacg tcgcgcaggc
      661 gaccgtcctg ctgcggcagg cggccggcat gggcctggcg atggcccaga acaacctcgg
      721 ccacctgctg agcggcaaca agtcggcgag cgacgacgcc gaggcgttcg gctggttcac
      781 gaaggccgcc gaacagggcg acatccaggc ccggtatgcc ctgggccagt gctactcgca
      841 gggccgcggg gtctcggccg acaagcgtgc ggcggccagg tggtaccgcg aagccgcgga
      901 acaaggcgtg cgcgaggcgc agttctcgct cggctacctc ctcgaactgg gcgacggcgt
      961 acccctggac cgggcgcagg cgttcgactg gtaccgcaag gccgccgacc agggcctgcc
     1021 cgaggcccaa tacaacctcg cgtcgctggc cgagcgcggc ggcagcccgg tgccgttgcg
     1081 ccccgaggag atcgtcggct ggtaccgcaa ggccgccgac aacggcctgc cgctggcgca
     1141 gaacaacctc gggcgccatt acgccacggg caccggcgtg ccgcgcgact tcgcgatggc
     1201 ggtcaccctg ttccggcagg cggcgcagca gggcgtggcg gaggcgcaga acaacctggg
     1261 gctcatgctc gccatgggca agggggtgcc gcgcgacgag cacgcggccg tcgagtggct
     1321 gcgccgctcc gccgcgcagg actatgacct cgcgcagatg aacctggcca acgcctacta
     1381 cgaagggcgc ggcgtggcag tcgaccgggc gctggcgtgc caatggatcc gccgcgccga
     1441 ggcgacgcac cgccggaccg cgacgccggc actgcccact gcgcaggccg gcgcgaatga
     1501 ccagatgctg gccaagtccc tcgcgcgcga ctgcgccggg cgctgagcgc ctcgctcagt
     1561 tgcgcaggct gcgcgccagc gtcggcgcgg cgtgcttggc gccgaacatg aagcgcatca
     1621 ccgggccgaa cgtcggcgcg ctggcggcgc ccacgatgtg caggcccggc acggacgtct
     1681 ggtagtggcg gtccagccgc ggcgcagccc cctctcgttc gatcgaggcc gacagcgccg
     1741 ggtcgaggta gtcgatgcgg tccaggtcga tccggaagcc ggtcgccgcg atcacgcggt
     1801 cggcgcgcag ttcctgttcg tcgccgtcct gccgcacggt cagggccacc ttgcccccgg
     1861 ccatccgcgc gtcgacgagc gtcgagccga acacggtggg gatgcggttc tccacccgcg
     1921 agcgcagcca ccacgcgccg gacggcccga agctctcgcc gacgtgccgg tggcgcttgt
     1981 gcggcgggaa catcgcgcgg tagacctgcg gcagctcggc gatcgcgatg tccttccacc
     2041 ccgggcccag gcccgaatac ggcttcttga tgcgatcgat ccagccgcgg ttcggcttgg
     2101 gcc
//
LOCUS       DATCRO010000766         8670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_818771, whole genome shotgun sequence.
ACCESSION   DATCRO010000766 DATCRO010000000
VERSION     DATCRO010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818771"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VIN75_20815"
     CDS             complement(<1..335)
                     /locus_tag="VIN75_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY8976669.1"
                     /translation="MPIYAYRCASCGHSRDVLQKISDPVLTTCPACGAEHFQKQVTAA
                     GFQLKGSGWYVTDFRNGGGTGAAAPAANPAPASGDAPAAAPAPAPAPSSDGGGSAAAS
                     SGGSCGTGAA"
     gene            complement(655..2238)
                     /gene="ubiB"
                     /locus_tag="VIN75_20820"
     CDS             complement(655..2238)
                     /gene="ubiB"
                     /locus_tag="VIN75_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY8976670.1"
                     /translation="MKHFARLLFITFTLLRFGVDEIALGRVRQPWLRGLARLLSIGRS
                     YDEPRGPRLRQALERLGPIFIKFGQVLSTRRDLLPADIADELALLQDRVPPFDGATAR
                     GLVEQGLLKELRRNFDDVFQDFDNTPVASASIAQVHFATLKGGREVAVKVLRPNVLQA
                     IESDLAILHTVARWVERISADGKRLRPREVVAEFDMYLHDELDLIREAANAAQLRRNM
                     ASLDLIMVPEMLWDFCTSRVMVMERMYGVPISQVERLRQAGVDIPKLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSIAPETFGRYIALDFGIIGTLTESDKDYLAQNFIAFFRRD
                     YKRVAELHIESGWVPPDTRLEALESAVRAVCEPHFDRPLKDISLGQVLLRLFQTSRRF
                     NVEIQPQLVLLQKTLLNVEGLGRQLDPELDLWSTAQPFLERWMNQQIGWRGFIDRLKD
                     EAPRYAHYIPALPRLVHQALTIAARKVEDELMPASAAQVNALLKEQRRTNRLLQAMLW
                     VAVGFVVGLCAARVWVALH"
     gene            complement(2273..2812)
                     /locus_tag="VIN75_20825"
     CDS             complement(2273..2812)
                     /locus_tag="VIN75_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976671.1"
                     /translation="MDLPFQDLWLPAVRNRMVLFANHVLTGCPPAVERLRAHAGKTIR
                     VDVTGWPGLVPVPPPLSLRVTPAGLFEGIEPAEEAGLVPDLQVRLDAADPAKAAGNVF
                     KGGLPPMSIDGDGALAADVSWIAQNVRWDPAADAERFFGPTVAEAVSRANEQFTQMGE
                     KVRSAVGQLFDQLQARKKS"
     gene            complement(2854..3474)
                     /locus_tag="VIN75_20830"
     CDS             complement(2854..3474)
                     /locus_tag="VIN75_20830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY8976672.1"
                     /translation="MPAARRVVAVFDFDGTVTDRDTLVPFLVAAFGRRRVAAAFAAQP
                     LAFAGTLLRVMPIDAFKRRVLRRLVKDTPAPRLRALGPAYARALASRVRPGALERIAW
                     HRAQGHLLVLASATLDVYLDHVGTQLGFDHVLCTRLASRPGDDGVDRLTGALEGRDCT
                     GPEKLRRLAALLGDLRAHELHAYGDSAGDRELLAAADHPHFQPFRR"
     gene            complement(3474..4496)
                     /gene="galE"
                     /locus_tag="VIN75_20835"
     CDS             complement(3474..4496)
                     /gene="galE"
                     /locus_tag="VIN75_20835"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8976673.1"
                     /translation="MATILLTGATGYIASHTWLELQAAGYDVVGLDDFSNSSPEVLNR
                     LEELSGRAPVFERASVCDADAMDAVFARHRIDAVVHFAAFKAVGESTQQPLRYYANNI
                     GGLLTLCTAMQAHGVRRIVFSSSATVYGKPETLPIREDAATSTTNPYGETKLIGEKIL
                     LDMTAADPTWQTGILRYFNPVGAHASGRIGEDPRGVPNNLMPYVAQVAIGRRPRLSVF
                     GDDYDTPDGTGVRDYIHVVDLAQGHVKALDKLLGAPGSVLVNLGTGRGYSVLELIAAY
                     EKASGKAVPYDIVARRPGDIDSCYADPAKALAEIGWKATRDLAAMCEDSWRWQTMNPG
                     GFDAVA"
     gene            complement(4501..5232)
                     /gene="ubiE"
                     /locus_tag="VIN75_20840"
     CDS             complement(4501..5232)
                     /gene="ubiE"
                     /locus_tag="VIN75_20840"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY8976674.1"
                     /translation="MSQTHFGYETVDETEKASKVRGVFDSVAKKYDVMNDLMSGGLHR
                     AWKAYTVAVANLKAGDKALDIAGGTGDLARAFARRVGPTGMVVHTDINEAMLRVGRDR
                     LIDEGLVLPTTICDAEKLPFPTGRFDLVSVAFGLRNMTHKDVALAEMNRVLRPGGRLL
                     VLEFSRIAAPLQKAYDWYSFNVLPRVGKVVAGDADSYRYLAESIRMHPDQATLKAMMK
                     TAGFGHVDVHNMAAGAVALHVGIKC"
     gene            complement(5243..5650)
                     /locus_tag="VIN75_20845"
     CDS             complement(5243..5650)
                     /locus_tag="VIN75_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY8976675.1"
                     /translation="MTSPDRPTPVALTVHQQSRALEIAFDDGRTFRIPFELMRVYSPS
                     AEVQGHGPGQETLQTGKRDVVIDAIEPVGHYAVKPTFSDGHDTGLFTWDYLYRLGAQQ
                     DELLTQYLERLAAAGLDRDAEMPKPGAGSCHSH"
     gene            5798..7057
                     /locus_tag="VIN75_20850"
     CDS             5798..7057
                     /locus_tag="VIN75_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976676.1"
                     /translation="MIRLLPLLALALSTPAFADLQKCLDAKGAPIYTDGDCPATGKQA
                     TAPAAVAAPRAGAGSAPVDAPLEILETGVPVITQMPGRFAWLDDDTLAITTFADAHAK
                     APWMVRRIVAYGVPTRSTGVLVPRGFIDCANAAHHLVSLEVGDLESRFAVGSRAAPAV
                     QQFATWDPATHKLGPAADAAGWHPGACLKPAPEDLGVHDLLASKKPLRYLEPEHGTLS
                     WGALDDSGHPAGPTLVTPRKKVPLAVTLSDISHDVRWLPFRKAYQLAPGVHDRLQDPP
                     HDAPLVTMDLDGRVTRHALPAGLVRQLDALSATGPAEMIATGAGDLVIQPGPAASGGG
                     LYVVQGEQSRRVWCTARPAPGQAGGADGCAMSQPVAVSPDGCRIAFDARPATPVANGF
                     PGAPTVKVLTLCDGSLPATAAGRKKGR"
     gene            complement(7126..7572)
                     /locus_tag="VIN75_20855"
     CDS             complement(7126..7572)
                     /locus_tag="VIN75_20855"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976677.1"
                     /translation="MRAQAMTAVLRQATRDDLAGIWRVRYAVSENTLAPGRIGNDEVI
                     AQLETTGRGWVVEEGGEVVAFAIGDASGANVWALFVDPRAEGRGHGSRLHDTMVEWLW
                     TNGLTTLWLTTGARTRARGFYERRGWRCTAELDDGQVRLELANAWS"
     gene            complement(7554..8042)
                     /locus_tag="VIN75_20860"
     CDS             complement(7554..8042)
                     /locus_tag="VIN75_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8976678.1"
                     /translation="MTARLRRLALATAVCAAGAAHAQLPVQPAANQEALLASADPALA
                     ANKRLVFDFWREVFEGGHLELADRYMAEGYIQHNPNVPTGRAAFVDFFKRFAKPKEIQ
                     PRIAAPLVSIVAERDLVILSFVQPTKDPKDASRTATTTWFDMFRIENGKIAEHWDCAP
                     RQ"
     gene            8114..8587
                     /locus_tag="VIN75_20865"
     CDS             8114..8587
                     /locus_tag="VIN75_20865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY8976679.1"
                     /translation="MPEFHPLSPPSFRPSCELCREAGGALVFQSAQWRVIRAEDAAFP
                     AFYRVVWRAHVAEWSDLTLAEQASMMGAVGAVERVLRDALAPTKINLASLGNVVPHLH
                     WHVIARFDWDSRFPAPVWAEPVRAVEPGAASRLALGLDELDARVARAVAALAPAA"
BASE COUNT         1206 a         3126 c         3092 g         1246 t
ORIGIN      
        1 gccgcgccgg tgccgcagga accgccggac gacgcggccg cactgccgcc gccgtcgctg
       61 gacggcgcgg gcgccggggc cggcgccgcg gccggggcgt cgcccgaggc gggcgccgga
      121 ttggcggcag gggcggccgc gccggtgccg ccgccgttgc ggaagtccgt gacataccag
      181 cccgagccct tgagctggaa accggcggcg gtcacctgct tctggaaatg ctcggctccg
      241 catgccggac aggtggtcag cacggggtcg gaaatcttct gcagcacgtc gcgcgaatgg
      301 ccgcacgagg cgcagcgata cgcgtagatc ggcatggctg ggctctctgg actaaacagg
      361 aaattataaa ggccgcctgc tccccggggc ttgaaacccg gtccggacga cccgcgagcc
      421 gtgaatatgg gggcttttcg tgacgaaaca agccgggcgg agcgagaaga gcggccctgg
      481 cagaggtgga tcctgcgact gcgcgcagga tgacgtctct tgcgcttccg tggcgacctc
      541 gcacgcgcga gcgagcggac tccttgtcgc ggagcgccaa tccagtgtca tcctgcgcgc
      601 agtcgcagga tccacgctgg ccgggcgccg gccgagcggc gaagccggtg ccgttcagtg
      661 caacgccacc cacacccgcg ccgcgcacag cccgacgacg aagcccactg cgacccacag
      721 catcgcctgc agcagccggt tggtgcggcg ctgctccttc agcagcgcgt tgacctgcgc
      781 cgcggaggcc ggcatcagct cgtcctcgac cttgcgggcc gcgatggtca gcgcctggtg
      841 caccagccgc ggcagcgccg ggatgtagtg ggcgtagcgc ggcgcctcgt ccttgaggcg
      901 gtcgatgaac ccgcgccagc cgatctgctg gttcatccag cgttccagga acggctgcgc
      961 cgtgctccac aggtcgagct cgggatcgag ctggcggccc aggccctcga cgttgagcag
     1021 cgtcttctgc aacagcacca gctgcggctg gatctcgacg ttgaagcggc gcgaggtctg
     1081 gaacagccgc agcagcacct ggcccagcga gatgtccttg agcgggcggt cgaagtgcgg
     1141 ctcgcacacg gcgcgcaccg cgctctcgag cgcctccagg cgcgtgtcgg gcggcaccca
     1201 gccgctctcg atgtgcagct cggccacgcg cttgtagtcg cggcggaaga acgcgatgaa
     1261 gttctgcgcg aggtagtcct tgtcggactc cgtgagcgtg ccgatgatgc cgaaatcgag
     1321 cgcgatgtag cgcccgaagg tctccggcgc gatgctcacc tggatgttgc cgggatgcat
     1381 gtccgcgtgg aagaagccgt cgcggaacac ctgcgtgaag aagatggtga cgccgtcgcg
     1441 cgccagcttg gggatgtcga cgccggcctg gcgcaggcgc tcgacctggc tgatgggcac
     1501 gccgtacatg cgctccatca ccatcacccg cgacgtgcag aagtcccaca gcatctcggg
     1561 caccatgatc aggtcgaggc tggccatgtt gcggcgcagc tgcgcggcgt tggcggcctc
     1621 gcggatcagg tcgagctcgt catgcaggta catgtcgaac tcggcgacca cctcgcgcgg
     1681 gcgcaggcgc ttgccgtcgg cgctgatgcg ctcgacccag cgggccaccg tgtgaaggat
     1741 ggcgaggtcg ctctcgatgg cctgcagcac gttgggccgc agcaccttga ccgccacctc
     1801 gcgcccgccc ttgagcgtgg cgaagtgcac ctgcgcgatc gacgcgctgg ccaccggcgt
     1861 gttgtcgaaa tcctggaaga cgtcgtcgaa attgcggcgc agctccttga gcaggccttg
     1921 ttcgaccagg ccgcgcgcgg tcgcgccgtc gaacggcggc acgcgatcct gcagcagcgc
     1981 gagttcgtcg gcgatgtcgg cgggcagcag gtcccgccgc gtcgacagca cctggccgaa
     2041 cttgatgaag atcgggccca gccgctccag cgcctggcgc aggcgcggcc cgcgcggctc
     2101 gtcgtagctg cggccgatgg acagcagccg ggccagcccg cgcagccacg gctggcgcac
     2161 gcgcccgagc gcgatctcgt cgacgccgaa ccgcagcagc gtgaacgtga tgaagagcag
     2221 acgcgcgaaa tgtttcatcg ggtcagtgcg ctcgcgcggc tggcctggag actcacgact
     2281 tcttgcgcgc ctgcaactgg tcgaacagct gccccacggc gctgcgcacc ttctcgccca
     2341 tctgcgtgaa ctgctcgttc gcgcggctga cggcctcggc cacggtcggg ccgaagaagc
     2401 gctcggcgtc ggcggccgga tcccagcgca cgttctgcgc gatccagctg acgtcggccg
     2461 ccagcgcgcc gtcgccgtcg atggacatgg gcggcaggcc gcccttgaac acgttgcccg
     2521 ccgccttggc cgggtcggcc gcgtccagcc tgacctgcag gtcgggcacc agcccggctt
     2581 cctcggcggg ctcgatgccc tcgaacaggc cggccggcgt gacccgcagc gacagcggcg
     2641 gcggcaccgg gaccagcccc ggccagcccg tgacgtcgac gcggatcgtc ttgcccgcat
     2701 gcgcgcgcag tcgctcgacc gccggcgggc agccggtcag cacgtggttg gcgaacagca
     2761 ccatgcggtt gcgcacggcg ggcagccaga gatcctggaa aggcaagtcc atgggggcga
     2821 ttgtaggtgg cgggcgttgc ccgacagtga cgctcagcgc cgaaacggct ggaaatgcgg
     2881 gtgatcggcg gccgcgagca gttcgcggtc gccggcgctg tcgccgtacg cgtgcaactc
     2941 gtgggcccgc aggtcgccca gcagcgcggc cagccgccgc agcttctccg ggccggtgca
     3001 gtcgcggccc tccagcgcgc cggtcaggcg gtcgacgccg tcgtcgccgg gccggctggc
     3061 caggcgcgtg cacagcacgt ggtcgaaacc cagctgcgtg ccgacatggt cgaggtagac
     3121 gtccagcgtc gcgctggcca gcaccagcag gtggccctgc gcgcggtgcc acgcgatgcg
     3181 ctcgagcgcg cccgggcgca cgcgtgacgc cagcgcgcgc gcgtacgcgg ggccgagcgc
     3241 acgcaggcgc ggcgcggggg tgtccttgac gaggcggcgc agcacgcggc gcttgaacgc
     3301 gtcgatcggc atgacgcgca gcagcgtgcc cgcgaacgcg agcggctggg cggcgaacgc
     3361 cgcggcgacg cgccggcggc cgaacgccgc cacgaggaag ggcacgagcg tgtcgcggtc
     3421 ggtgaccgtg ccgtcgaagt cgaagaccgc gacgacgcgg cgcgcggccg gcatcacgcc
     3481 accgcgtcga acccgcccgg attcatcgtc tgccagcgcc agctgtcctc gcacatcgcc
     3541 gcaaggtcgc gcgtggcctt ccagccgatt tcggcgagcg ccttggccgg gtcggcgtag
     3601 cagctgtcga tgtcgccggg acggcgcgcg acgatgtcgt agggcacggc cttgccgctt
     3661 gccttctcgt acgcggcgat cagctccagc acgctgtagc cgcggccggt gcccaggttg
     3721 accagcaccg agccgggcgc gcccagcagc ttgtccagcg ccttgacgtg gccctgcgcg
     3781 aggtcgacca cgtggatgta gtcgcgcacg ccggtgccgt cgggggtgtc gtagtcgtcg
     3841 ccgaacacgg acagccgcgg gcgccggccg atggccacct gcgccacgta cggcatcagg
     3901 ttgttgggca cgccgcgcgg gtcctcgccg atgcgcccgc tcgcatgcgc gcccaccggg
     3961 ttgaagtagc gcaggatgcc ggtctgccac gtcggatcgg cggcggtcat gtccagcagg
     4021 atcttctcgc cgatcagctt ggtctcgccg tacgggttgg tcgtgctggt ggccgcgtcc
     4081 tcgcggatcg gcagcgtctc gggcttgccg tacacggtcg cgctcgagct gaagacgatg
     4141 cgcctgacgc cgtgtgcctg catcgccgtg cacagcgtga gcagcccgcc gatgttgttg
     4201 gcgtagtagc gcagtggctg ctgcgtcgat tcgcccaccg ccttgaaggc cgcgaagtgg
     4261 acgacggcgt cgatgcgatg ccgcgcgaag acggcgtcca tcgcgtcggc gtcgcagacg
     4321 ctcgcgcgct cgaacaccgg cgccctgccc gacagctcct cgagccggtt gagcacctcg
     4381 ggcgagctgt tggagaagtc gtccaggccg acgacgtcgt agccggcggc ctgcagttcg
     4441 agccaggtgt gggaggcgat gtagccggtc gcgccggtca gcaggatcgt ggccatgcgg
     4501 tcagcacttg atgccgacgt gcagcgccac ggcgccggca gccatgttgt gcacgtcgac
     4561 gtggccgaag cccgcggtct tcatcatcgc cttgagcgtc gcctggtcgg ggtgcatgcg
     4621 gatcgactcg gccaggtagc ggtagctgtc ggcgtcgccg gccacgacct tgcccacgcg
     4681 cggcagcaca ttgaacgaat accagtcgta ggccttctgc agcggcgcgg cgatcctgga
     4741 gaactcgagc accagcaggc ggccgcccgg acgcagcacg cggttcatct cggccagcgc
     4801 gacatccttg tgcgtcatgt tgcgcaagcc gaaggccacg ctgacgaggt cgaaccggcc
     4861 ggtggggaac ggcagcttct cggcgtcgca gatcgtcgtg ggcagcacca ggccctcgtc
     4921 gatcaggcgg tcgcggccga cgcgcagcat cgcctcgttg atgtcggtat gcacgaccat
     4981 gccggtgggg ccgacgcgcc tggcgaacgc ccgcgccagg tcgccggtgc cgccggcgat
     5041 gtccagcgcc ttgtcgcccg ccttgaggtt ggcgaccgcc acggtgtagg ccttccatgc
     5101 gcggtgcagg ccgcccgaca tgaggtcgtt catcacgtcg tacttcttgg cgacggagtc
     5161 gaacaccccg cgcaccttgc tcgccttctc ggtctcgtcg acggtctcgt agccgaagtg
     5221 ggtttgactc atgggaattc tttcagtgtg aatggcacga cccggcgccc ggcttgggca
     5281 tctcggcatc gcggtcgagg ccggcggcgg ccaggcgctc gaggtactgg gtcagcagct
     5341 cgtcctgctg cgcgcccagg cggtagaggt agtcccaggt gaacaggccg gtgtcgtggc
     5401 catcggagaa cgtgggcttg accgcatagt gtcccaccgg ctcgatggcg tcgatgacga
     5461 cgtcgcgctt gcccgtctgc agcgtctcct ggccggggcc gtggccctgg acctcggccg
     5521 acggcgaata gacgcgcatc agctcgaacg ggatgcggaa cgtgcgcccg tcgtcgaacg
     5581 cgatctccag cgcgcgcgac tgctggtgca cggtgagcgc gacgggcgtg gggcggtcgg
     5641 gggaggtcat cccgccagtt tatctttcgc cgcccgcccc ccgggcagga cgggttcgcg
     5701 gagacggccg gcctccccca caagggggac accggtgttc gtaacaaggt gtgagaatcc
     5761 agcgtttcga acatacccgt ccccggcatc cgcacccatg atccgtctcc ttccgctgct
     5821 ggccctggcg ctgtcgactc ccgccttcgc cgacctgcag aaatgcctcg acgccaaggg
     5881 cgcgccgatc tacaccgacg gggactgccc cgccaccggc aagcaggcga cggcgccggc
     5941 cgccgtcgcc gcgcccaggg caggcgccgg cagtgcgccg gtggacgccc cgctggagat
     6001 cctcgagacc ggcgtgccgg tcatcacgca gatgccgggc cgcttcgcct ggctggacga
     6061 cgacaccctg gccatcacca ccttcgccga cgcccacgcc aaggcgccct ggatggtgcg
     6121 ccgcatcgtc gcgtacggcg tgccgacgcg cagcaccggc gtgctggtgc cgcgcggctt
     6181 catcgactgc gccaacgccg cccaccacct cgtcagcctg gaggtcggcg acctcgaatc
     6241 gcgctttgcc gtcggctcgc gcgcggcccc ggccgtgcag cagttcgcca cctgggaccc
     6301 ggccacgcac aagctggggc cggccgccga cgccgccggc tggcacccgg gcgcctgcct
     6361 gaagcccgca cccgaggacc tgggcgtgca cgacctgctg gccagcaaga agccgctgcg
     6421 ctacctcgag cccgagcacg gcacgctcag ctggggggca ctggacgaca gcggccatcc
     6481 ggccgggccg acgctggtca cgcccaggaa gaaggtgccc cttgccgtga ccctgtccga
     6541 catctcgcac gacgtgcgct ggctgccgtt ccgcaaggcc taccagctgg cgccgggcgt
     6601 ccacgaccgc ctgcaggatc cgccgcacga cgccccgctg gtcacgatgg acctcgacgg
     6661 ccgcgtcacg cgccatgcgc tgcccgcggg cctggtgcgc cagctcgatg cgctgtccgc
     6721 gaccggaccg gccgagatga tcgccaccgg cgcgggcgac ctcgtgatcc agccgggccc
     6781 ggccgccagc ggcggcggcc tctacgtcgt ccagggcgag cagagccggc gcgtgtggtg
     6841 caccgcccgg cccgcgccgg gccaggccgg cggcgccgac ggctgcgcga tgtcgcagcc
     6901 ggtggcggtc tcccccgacg gctgccgcat cgcgttcgac gcccgcccgg cgaccccggt
     6961 cgccaacggc ttcccgggcg cgccgacggt caaggtgctg acgctgtgcg acggcagcct
     7021 gccggcgacc gccgcgggtc gcaagaaggg ccgctgacgg ctggtgtgct gcccggggag
     7081 tccgcacgcg cgatacggct gcgcgcgtac ttccgggtga gcacactagg accaggcgtt
     7141 cgccagctcc agccgcacct gcccgtcgtc gagctcggcc gtgcagcgcc agccgcggcg
     7201 ctcgtagaag ccgcgcgccc gggtgcgggc gccggtggtc agccacagcg tggtcaggcc
     7261 gttcgtccac agccactcga ccatcgtgtc gtgcagccgc gagccgtggc cgcggccttc
     7321 cgcgcgcggg tcgacgaaca gcgcccacac gttggcgccg ctggcgtcgc cgatcgcgaa
     7381 ggcgaccacc tcgccgcctt cctccacgac ccagccgcgg ccggtcgtct ccagctgggc
     7441 gatcacctcg tcgttgccga tgcgtcccgg cgcgagcgtg ttctcggaaa cggcgtagcg
     7501 cacccgccag atgccggcga ggtcgtcgcg ggtggcctgg cgcagcaccg cggtcattgc
     7561 ctgggcgcgc aatcccagtg ctcggcgatc ttgccgttct cgatgcggaa catgtcgaac
     7621 cacgtcgtcg tggccgtcct ggacgcgtcc ttcggatcct tggtgggctg cacgaagctc
     7681 aggatcacga ggtcgcgctc ggccacgatc gagaccagcg gcgcggcgat gcgcggctgg
     7741 atttccttcg gcttggcgaa gcgcttgaag aagtcgacga acgccgctcg gccggtgggc
     7801 acgttcgggt tgtgctggat atagccctcg gccatgtacc tgtcggccag ctccaggtgc
     7861 ccgccctcga agacctcgcg ccagaaatcg aacaccagcc tcttgttggc ggccagcgcg
     7921 ggatcggcac tggccagcag cgcctcctgg ttcgcggccg gctgcacggg caactgcgcg
     7981 tgcgcggcgc cggcggcgca cacggcggtg gcgagcgcga ggcggcgcag gcgggctgtc
     8041 acacaatacc gcatcgcgcg gccggctcgc caccggagcg gcccgcaatc ccgctccttc
     8101 cgccgccgcc gccatgcccg agttccaccc gctgtccccg ccctcgttcc ggccgtcctg
     8161 cgagctgtgc cgcgaggccg gcggcgcgct cgtcttccag tcggcccagt ggcgcgtcat
     8221 ccgcgccgag gacgccgcct tcccggcgtt ctaccgcgtc gtctggcgcg cgcacgtggc
     8281 cgagtggagc gacctcacgc tcgccgagca ggcctcgatg atgggcgccg tgggcgccgt
     8341 cgaacgcgtg ctgcgcgacg cgctcgcgcc caccaagatc aatctcgcca gcctgggcaa
     8401 cgtggtgccg cacctgcact ggcatgtgat cgcccgcttc gactgggaca gccgcttccc
     8461 cgcgccggtc tgggccgagc cggtgcgcgc ggtcgagccc ggcgccgcaa gccggctcgc
     8521 gctcggcctc gacgagctcg acgcccgcgt ggcgcgcgcc gtcgcggccc tcgcgcccgc
     8581 cgcctgaccc gtcgacccgg aaggacgcca tgaccgagac gatcctgcgc caggccaccc
     8641 gcgacgacgc cgccggcctc tggcgcgtgc
//
LOCUS       DATCRO010000767         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1009925, whole genome shotgun sequence.
ACCESSION   DATCRO010000767 DATCRO010000000
VERSION     DATCRO010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009925"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..810)
                     /locus_tag="VIN75_20870"
     CDS             complement(1..810)
                     /locus_tag="VIN75_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976680.1"
                     /translation="MIAFHFGPAERPLFGLFHPAEGGSPDDRAVLLCNPYGQEAVRSH
                     RFYRVLAERLARMGLATLRFDYHATGDSPGDDLAGEIPGWVGDTRAALRELVSRSGAR
                     RVVLVGARLGAVMAVRASAGLAELRRIVLWDPIVNGQDYLELLRVKHVEALESSFSLA
                     DPAWREQLAGDPAAFTDEAMGFAMSPALRQQIRQVGPHSLPLPPGVPVQVIVREDDAA
                     VQAWTATFAPRGIEFRSWPLAESFDWTAEEDRNSPLVPPPALAAMMKAIRD"
     gene            complement(888..5858)
                     /locus_tag="VIN75_20875"
     CDS             complement(888..5858)
                     /locus_tag="VIN75_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8976681.1"
                     /translation="MSEANLEDAGVSLEHDPFGAGQVERVVPTTEAQREIWLADQLGR
                     EASLAYNESASLRFEGPLDEAALDAALAFLGERHEALHSTVGPDGTELWVSKAVPLRA
                     EKLDFAALDTVQRAQSMAAEREKAVETRFDVATGPLIRAVLMRAGDQQHELMLTAHHI
                     VCDGWSLGVLAEELMAIYKAFAAGEPSMLEDAPRYGDHALAQRQPEALLQQAEDERYW
                     VGLYDRSIPVLDLPSDRSRLATHRGFASRREDLAIDATLAEAVRRNGARAGASLFATL
                     FSLYAATLARLAGVDELVVGVPAAGQPTAGLNGLVGHCVQLLPIRVAVDIDQGADAQI
                     AATRGAVLDAYDHQGCTFGSLLKKLNIERDPARLPLVSVLFNLDAKIDPAALSTDDLR
                     VHVVGNPRRYENFELFLNVAQVDGGLQLECQYNTGLFDGATVRRWLALYKSALERYAA
                     DPAVTLASALAPAGEEQAILERFNATRLEHDRSLRVDTLIARQAAATPDAVAVVSGST
                     HLSYRELDTRANALAADLQARGIGPGELVGLCCDRNENMVVALLGVLKSGAGYVPLDP
                     ALPRQRLEFMAQDAELRFVASDFDAAAAWPFAVAQRVPVAGLAPRAEAPACAAGVDDV
                     AYVIYTSGSTGTPKGVRVPHRSVVNLLASLRTTPGMRASHRVLCVTTLSFDIAVSEVI
                     MPLTVGARIVLATRAQAMDGEQLRGLIEREGVDFIDGTPSTWQLLLAAGWPGRHDLLA
                     ICTGEPLPPELGSRLLPLVGQLWNGYGPTETTVWSSFHRVERVDGPVPIGRPIANTQF
                     HVADSRLRPLPLGAVGELFIAGEGVTLGYLNRPELNAERFLAAPGGAAGRWYRTGDLG
                     RWRADGILECLGRIDHQVKVRGYRIELGEIEAVLARHPALERALVITREDQPGDVRLV
                     AYVIGRAAFEDADLRRHLGESLPEYMIPQHFVRITAIPALANGKLDRRALPKPTVDAA
                     ARSVERVAPANDTEQRVLAAMEAILDLPGLSMLDNFFALGGHSLLAMRLTTKLNGEFG
                     IALPLRTLFEASTARALAAAVDRAPRRGEPARAAIVHAGDQRSAPLTLPQERIRFLEE
                     LHPGRVVFNTPSGHRLTGTMDVAVFEQALREVVRRQPVLRTRIARSEDGEGFVQRIED
                     GIDFALPFEDLSAIPAERREAELLARMQAIIDTPIPIDQAPLFRVALWKLAPEEHAFL
                     FMPHHIIWDGWSFDILYDEMSAVYGALSTATPIALAAPAISFADYARWLGDWMRGPDC
                     ERQLQYWKKRFARMPMPKAPATDMPRNPGMSGVGATEWVRIDAELTARLRDVARRHDL
                     TLSMLAMSVYAAMMASAIDGRSIVVGLPVRGRLSPETEGVMGFFNNLLPVQFEFEPGK
                     TLGEFLDAVKKDTLDVFEHQEIPFERLASEPEISSRSQRVGLYQALFSFQDARDRKRD
                     WGALHQKSILVFQKGATEDLGLWLMEVPGGLEGGFTYNADIYLPETAAAFRERYLELL
                     RRIAGDSSLKMADFTDVGGSSAGRLLRRLSGDGAAAPAPVPAAAPASRPAEAFASDNE
                     RALARIWSELLNVEVSHVSPRDNFFDLGGDSLLAMRAVEAAGRTLGFRIDARRYFYES
                     LAQLANPQAAAAVAPAAQAAAGRAEAEQGGGLLKRVFGAFGGKGRK"
     gene            complement(5937..>7100)
                     /locus_tag="VIN75_20880"
     CDS             complement(5937..>7100)
                     /locus_tag="VIN75_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8976682.1"
                     /translation="PLAGEVADLICEMTGNDRVGLCNTGSEAVMAAIRIARTVTCRNT
                     VVLFTGSYHGTFDEVLVRAGRNHKGIPAAPGIMADVFDDVRVLDYGTPEALEFIRANA
                     DDLAAVLVEPVQSRRPDFQPREFLKQVREITEASGTCLIFDEVITGFRMHPGGVQAMF
                     DIRADLTTYGKVVGGGYPIGVIAGKRHFMDALDGGPWQYGDDSVPSVGVTYFAGTFVR
                     HPLALAAAKAALLHLKEQGGKLQEQLNLSTAAMADELTAFCREVGAPIEIRHFGSLWR
                     VSWQEDHPLQDLLFAMMRSRGVHILDNFPCFMTTAHTPQDIARIKQAFKESVAELQQA
                     EFLPARAAAVPAAVDASKPPVPGARLGRGPNGEVQWYVPHPDQPGKFVVATNA"
BASE COUNT          977 a         2541 c         2608 g          974 t
ORIGIN      
        1 tcagtcacgg atggccttca tcatcgcggc cagcgccggg ggcggcacga gcgggctgtt
       61 gcggtcttcc tcggccgtcc agtcgaacga ttcggcgagc ggccacgacc ggaactcgat
      121 gccgcgcggc gcgaaggtgg ccgtccaggc ctgcaccgcc gcgtcgtcct cgcgcacgat
      181 cacctgcacc ggcacgccgg gcggcagcgg caggctgtgc gggccgacct ggcggatctg
      241 ctggcgcagc gccggcgaca tcgcgaagcc catcgcctcg tcggtgaacg ccgccgggtc
      301 gccggccagt tgctcgcgcc acgccgggtc ggccaggctg aacgaggact cgagcgcctc
      361 cacgtgcttg acgcgcagca gctcgaggta gtcctggcca ttgacgatgg gatcccacag
      421 cacgatgcgc cgcagttcgg ccaggcccgc ggacgcgcgc accgccatca ccgcaccgag
      481 ccgcgcgccc acaaggacga cgcgccgcgc gccgctgcgc gacacgagct cgcgcagggc
      541 ggcgcgggtg tcgccgaccc agccggggat ctcgcccgcc aggtcgtcgc cgggcgaatc
      601 gccggtggcg tggtagtcga agcgcagcgt cgcgaggccc atgcgggcca ggcgctcggc
      661 cagcacgcga tagaagcgat ggctgcgcac ggcttcctgg ccgtacggat tgcacagcag
      721 cacggcgcga tcgtcgggcg agccgccctc cgccggatgg aacaggccga acagcggccg
      781 ctcggccggc ccgaagtgga acgcgatcat ccgcggattg tcggcgcgat ccattcagcc
      841 tggagtcagg acaacaccgg gcaggccgcc ggctccggca ataccgctca tttgcggccc
      901 tttccgccga acgccccgaa cacgcgcttg agcaggccgc cgccctgctc cgcctccgcg
      961 cggcccgccg cggcctgggc agcgggcgcg accgcggccg ccgcctgcgg gttggccagc
     1021 tgcgcgaggc tctcgtagaa gtagcggcgc gcatcgatgc ggaagccgag cgtgcggccc
     1081 gccgcctcga cggcacgcat cgccagcagc gagtcgccgc cgaggtcgaa gaagttgtcg
     1141 cgcggcgaga cgtgcgacac ctcgacgttc agcagctcgg accagatgcg cgccagcgcg
     1201 cgctcgttgt cggacgcgaa cgcctcggcc ggccggcttg ccggcgcggc ggcgggcacg
     1261 ggcgccgggg cggcggcgcc gtcgcccgac aggcgccgca gcagccggcc cgcgctcgac
     1321 ccgccgacgt cggtgaagtc ggccatcttc agcgacgagt cgcctgcgat gcggcgcagc
     1381 agttcgaggt agcgttcgcg gaacgcggcc gccgtctcgg gcaggtagat gtcggcgttg
     1441 taggtgaagc cgccctcgag gccgcccggc acctccatca gccacaggcc caggtcctcg
     1501 gtcgcgccct tctggaacac gaggatggac ttctggtgca gcgcgcccca gtcgcgcttg
     1561 cggtcgcgcg cgtcctggaa cgagaacagc gcctggtaca ggccgacgcg ctggctgcgg
     1621 ctggagatct cgggttcgga cgcgaggcgc tcgaagggga tctcctggtg ctcgaacacg
     1681 tcgagcgtgt ccttcttgac cgcgtcgagg aactccccga gcgtcttgcc cggctcgaac
     1741 tcgaactgca ccggcagcag gttgttgaag aagcccatca cgccctcggt ctcgggcgac
     1801 aggcggccac gcaccggcag ccccacgacg atcgagcgcc cgtcgatggc cgaggccatc
     1861 atcgcggcgt agaccgacat cgccagcatg ctcagcgtga gatcgtggcg gcgcgcgacg
     1921 tcgcgcaggc gggcggtgag ctccgcgtcg atgcgcaccc actcggtcgc gccgacgccg
     1981 ctcatgcccg ggttgcgcgg catgtcggtg gcgggcgcct tgggcatggg catgcgcgcg
     2041 aagcgcttct tccagtactg cagctggcgc tcgcagtcgg gcccgcgcat ccagtcgccc
     2101 agccagcggg cgtagtcggc gaagctgatg gcgggcgccg ccagcgcgat cggcgtggcg
     2161 gtcgacagcg cgccgtagac ggccgacatc tcgtcgtaca ggatgtcgaa cgaccagccg
     2221 tcccagatga tgtggtgcgg catgaacagg aacgcgtgct cctcgggcgc cagcttccac
     2281 agcgcgacgc ggaacagcgg cgcctggtcg atggggatgg gcgtgtcgat gatcgcctgc
     2341 atgcgcgcga gcagctcggc ctcgcgccgc tccgcgggga tcgccgacag gtcctcgaac
     2401 ggcagcgcga agtcgatgcc gtcctcgatg cgctgcacga agccctcgcc atcctccgaa
     2461 cgcgcgatgc gcgtgcgcag caccggctgg cgccgcacca cttcgcgcag cgcctgctcg
     2521 aagacggcca cgtccatggt gccggtcagg cggtggcccg acggcgtgtt gaacacgacg
     2581 cggcccggat gcagttcctc caggaagcgg atgcgttcct gcggcagcgt cagcggcgcg
     2641 ctgcgctggt cgcccgcgtg cacgatggcc gcgcgcgccg gctcgccgcg gcgcggcgcg
     2701 cggtcgacgg cggcggccag cgcccgcgcg gtcgacgcct cgaacagcgt gcgcagcggc
     2761 agcgcgatgc cgaactcgcc gttgagcttc gtggtcagcc gcatcgccag cagcgaatgg
     2821 ccgccgagcg cgaagaagtt gtccagcatg ctcaggcccg gcaggtccag gatcgcctcc
     2881 atcgcggcga gcacgcgctg ctcggtgtcg ttggcgggcg ccacgcgctc gacgctgcgc
     2941 gcggccgcgt ccaccgtggg cttgggcagc gcgcgccggt cgagcttgcc gttggccagc
     3001 gccgggatcg ccgtgatgcg cacgaagtgc tgcgggatca tgtattcggg cagcgactcg
     3061 cccaggtggc ggcgcaggtc ggcgtcctcg aacgccgcgc ggccgatcac gtaggccacc
     3121 aggcgcacgt cgcccggctg gtcctcgcgc gtgatcacca gggcgcgctc gagcgccggg
     3181 tggcgcgcca gcacggcctc gatctcgccc agctcgatgc ggtagccgcg caccttgacc
     3241 tggtggtcga tgcggcccag gcattccagg atgccgtcgg cgcgccagcg gcccaggtcg
     3301 cccgtgcggt accagcggcc ggcggcgccg cccggcgcgg cgaggaagcg ctcggcattc
     3361 agctcgggcc ggttcaggta gccgagggtg acgccttcgc cggcgatgaa cagctcgccg
     3421 accgcgccca gcggcagcgg gcgcaggcgg ctgtcggcca cgtggaactg cgtgttggcg
     3481 atcgggcggc cgatcggcac cgggccgtcg acgcgctcga cgcgatggaa gctggaccac
     3541 accgtggtct cggtcgggcc gtagccgttc cacagctggc ccaccagcgg gagcaggcgg
     3601 ctgcccagct cggggggcag cggctcgccg gtgcagatcg ccagcaggtc gtggcggccc
     3661 ggccagccgg cggccagcag cagctgccag gtggacggcg tgccgtcgat gaagtccacg
     3721 ccttcgcgct cgatgaggcc gcgcagctgc tcgccatcca tcgcctgcgc gcgggtggcc
     3781 agcacgatgc gcgcgccgac cgtcagcggc atgatgacct cggagacggc gatgtcgaac
     3841 gacagcgtcg tgacgcacag cacgcgatgc gacgcgcgca tgcccggcgt cgtgcgcagg
     3901 ctggccagca ggttgaccac cgagcggtgc ggcacgcgca cgcccttggg agtgccggtc
     3961 gagcccgagg tgtagatcac gtaggcgacg tcgtcgacgc cggcggcgca ggccggagcc
     4021 tccgcgcgcg gcgcgaggcc cgccaccggc acacgttgcg ccaccgcgaa cggccacgcc
     4081 gccgcggcgt cgaagtcgct ggccacgaag cgcagctcgg cgtcctgggc catgaactcc
     4141 aggcgctggc gcggcagggc cgggtcgagc ggcacgtagc cggcgccgct cttgagcacg
     4201 cccagcagcg cgacgaccat gttttcgttg cggtcgcagc acaagcccac cagctcgccg
     4261 gggccgatgc cgcgcgcctg caggtcggcg gccagcgcgt tggcgcgggt gtcgagctcg
     4321 cggtagctca ggtgcgtgga gccgctgacg acggcgaccg cgtccggcgt ggccgcggcc
     4381 tggcgtgcga tgagcgtgtc gacgcgcagg ctgcggtcgt gctcgagccg ggtcgcgttg
     4441 aagcgctcga ggatcgcctg ctcctcgccg gcgggcgcga gcgcggacgc cagcgtgacc
     4501 gccggatccg ccgcgtagcg ttcgagcgcg ctcttgtaaa gggcgagcca gcgccgcacc
     4561 gtggcgccgt cgaacaggcc ggtgttgtac tggcattcga gctgcaggcc gccgtcgacc
     4621 tgggcgacgt tcaggaacag ctcgaagttc tcgtagcggc gcgggttgcc cactacgtgc
     4681 acgcgcaggt cgtcggtgga cagcgccgcc gggtcgatct tggcgtcgag gttgaacagc
     4741 accgagacca gcggcaggcg ggccggatcg cgctcgatat tcagcttctt gagcaggctg
     4801 ccgaaggtgc agccctggtg gtcgtaggcg tccagcacgg cgccgcgggt ggcggcgatc
     4861 tgggcgtcgg cgccctggtc gatgtcgacg gccacgcgga tcggcagcag ctgcacgcaa
     4921 tggccgacga gtccgttcag gccggccgtg ggctggcccg cggcgggcac gcccaccacc
     4981 agctcgtcga cgcccgccag gcgcgccagc gtcgcggcgt acagcgagaa cagcgtggcg
     5041 aacaggctgg cgccggcgcg cgcgccgttg cgccgcaccg cctcggcgag cgtcgcatcg
     5101 atggccaggt cctcgcggcg cgacgcgaag ccgcggtgcg tggccaggcg cgagcggtcg
     5161 ctgggcaggt ccagcaccgg gatcgaacgg tcgtagaggc cgacccagta gcgctcgtcc
     5221 tcggcctgct gcagcagcgc ctcgggctgg cgctgcgcga gcgcgtggtc gccgtagcgc
     5281 ggcgcgtcct ccagcatcga cggctcgccg gcggcgaacg ccttgtagat cgccatcagc
     5341 tcctcggcca gcacgcccag cgaccagcca tcgcacacga tgtggtgcgc ggtgagcatc
     5401 agctcgtgct gctgatcgcc cgcgcgcatc agcaccgcgc ggatcagcgg gccggtggcg
     5461 acgtcgaaac gcgtctccac ggccttctcg cgctcggccg ccatggactg cgcgcgctgc
     5521 acggtgtcga gcgcggcgaa gtccagcttc tcggcgcgca gcggcaccgc cttggacacc
     5581 cacagctcgg tgccgtccgg ccccacggtc gaatgcagcg cctcgtggcg ctcgccgagg
     5641 aaggccagcg cggcatcgag cgcggcctcg tccagcgggc cttcgaagcg cagcgaggcg
     5701 gactcgttgt aggccaggga cgcctcgcgg cccagctggt cggccagcca gatctcgcgc
     5761 tgcgcctccg tggtcgggac gacacgctcc acctggcccg cgccgaacgg atcgtgctcc
     5821 agcgacacgc ccgcatcttc cagatttgcc tcgctcattc gtctttttcc ttcagcgtcg
     5881 ggcgcccttg tcgccgcgtc ggccgtgttg tttcatgggg cggtgccccg ttgcgttcaa
     5941 gcgttcgtcg cgacgacgaa cttgcccggc tggtcgggat ggggcacgta ccactgcacc
     6001 tccccgttgg ggccgcgccc gagccgcgcg cccggcacgg gcggcttgga cgcgtcgacc
     6061 gccgccggca ccgccgcggc gcgcgcgggc aggaactcgg cctgctgcag ctcggccacc
     6121 gattccttga acgcctgctt gatcctggcg atgtcctgcg gcgtgtgcgc ggtcgtcatg
     6181 aagcacggga agttgtcgag gatgtgcacg ccgcggctgc gcatcatcgc gaacagcagg
     6241 tcctgcagcg gatggtcttc ctgccagctg acgcgccaca gcgatccgaa gtggcggatc
     6301 tcgatcggcg cgccgacctc gcggcagaac gcggtgagct cgtcggccat cgccgcggtg
     6361 ctcaggttca gttgctcctg cagcttgccg ccctgctcct tcaggtgcag cagcgccgcc
     6421 ttggccgccg cgagcgccag cgggtggcgc acgaaggtgc cggcgaagta ggtgacgccc
     6481 acgctgggca cggagtcgtc gccgtactgc cacgggccgc cgtccagcgc gtccatgaag
     6541 tggcgcttgc cggcgatcac gccgatcggg tagccgcctc caaccacctt gccgtaggtc
     6601 gtgaggtcgg cgcggatgtc gaacatcgcc tgcacgccgc ccgggtgcat gcggaagccc
     6661 gtgatgacct cgtcgaagat caggcaggtg ccgctcgcct cggtgatctc gcgcacctgc
     6721 ttgaggaact cgcgcggctg gaagtcgggg cgccggctct gcaccggctc gaccagcacg
     6781 gcggccaggt cgtcggcatt ggcgcggatg aactcgagcg cctcgggggt gccgtaatcc
     6841 agcacgcgca cgtcgtcgaa cacgtcggcc atgatgcccg gcgcggccgg gatgcccttg
     6901 tggttgcggc cggcgcgcac cagcacctcg tcgaaggtgc cgtggtacga gcccgtgaac
     6961 agcaccaccg tgttgcggca cgtgacggtg cgcgcgatgc ggatggccgc catcacggcc
     7021 tcggagccgg tgttgcacag gccgacgcgg tcgttgccgg tcatctcgca gatcaggtcg
     7081 gccacctcgc cggccagcgg
//
LOCUS       DATCRO010000768         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_650014, whole genome shotgun sequence.
ACCESSION   DATCRO010000768 DATCRO010000000
VERSION     DATCRO010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650014"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /gene="ligD"
                     /locus_tag="VIN75_20885"
     CDS             <1..1999
                     /gene="ligD"
                     /locus_tag="VIN75_20885"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY8976683.1"
                     /translation="AKTAAPALDPAALDGARKATLPETLSPQLATLAKAAPTHGDWLY
                     EIKFDGYRLMTRIARGKVAIITRGGHDWTAKLLPLAEELAALGIDNAWLDGEAVVLDA
                     SGLPQFNLLQNALDSRRSAEAIVYYVFDAPFINGFDLRGVPLHARRALLKQVIDAHRG
                     DKVRFSDDFPANAGEVLATACNLKLEGVIAKRRDGLYTSSRSTDWLKLKCQQRQEFVI
                     GGFSDRSDNAKAIGALTLGYYDEHGKLQPAGRVGTGWSSADAVTLRTKLAKLVTDKSP
                     FHVGATRSTRWMTRPAAEDHWVRPQLVAEVSFAEWTPDGSIRHASFQGLREDKAARDV
                     GRERAKAPPTSEKAVTATKTSRTPAAPAAKTSAKAGSAEVEGIRISHPERVVDAASGH
                     TKLDLARYYASIAEFMLPHLKSRPVSLVRAPEGVGHELFFQKHADVRTMPGVKDLYGL
                     WEGHDPLIEIPSAKALIAAAQMNVIEFHTWNSVKQKIDKPDRMIFDLDPGDGVKFDQV
                     REGAQLMRALLEELGLRCWLKTSGGKGLHVVVPMSARLDYDTVKAFSQRIVQHMAATI
                     PQRFVAKSGGSNRVGKIFIDYLRNGFNATTAAAFSARARPGLGVSIPIAWDELAQIRG
                     GDHWTISNARDRMSFQKADPWAEYWSCRQTMTAAMKALDAG"
     gene            2115..>2417
                     /locus_tag="VIN75_20890"
     CDS             2115..>2417
                     /locus_tag="VIN75_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8976684.1"
                     /translation="MDFKDYVACDATRLAELVARREVGAHELLELALTHLRRTQPRIN
                     AVCRLMEAEARAQLAAPLPAGPFAGVPFPQAIQVLHAGHGAHIGVLLEEQLVPDALG"
BASE COUNT          401 a          850 c          836 g          330 t
ORIGIN      
        1 ggcaaagacg gcggcaccgg cactcgatcc cgccgcactg gacggcgcgc gcaaggccac
       61 gttgcccgag acgctgtccc ctcaactggc cacgcttgcc aaggccgcgc ccacgcacgg
      121 cgactggctc tacgagatca agttcgacgg ctatcgcctg atgacgcgca tcgccagggg
      181 caaggtggcc atcatcacgc gcggcggcca cgactggacg gccaagctgc tgccgttggc
      241 cgaggagctc gccgccttgg gcatcgacaa cgcctggctc gacggcgaag ccgtggtcct
      301 cgacgcgagc ggcctgccgc agttcaacct gctgcagaac gcgctcgact cgcgccgcag
      361 cgccgaggcg atcgtctact acgtgttcga cgcgccgttc atcaacggct ttgacctgcg
      421 cggcgtgccg ctgcacgcgc ggcgcgcgct gctcaagcag gtgatcgacg cccatcgagg
      481 cgacaaggtg cgcttcagcg acgatttccc cgccaacgcg ggcgaggtgc tggccacggc
      541 gtgcaacctg aagctcgaag gcgtgatcgc caagcgccgc gacgggctgt acacgtcgtc
      601 gcgctcgacc gactggctca agctcaagtg ccagcagcgc caggaattcg tgatcggcgg
      661 cttcagcgac cgcagcgaca acgcgaaggc gatcggcgcg ctgacgctgg gttactacga
      721 cgagcacggc aagctgcagc ctgcgggtcg cgtgggcacg ggctggagct cggccgatgc
      781 ggtgacgctg cgcaccaagc tggccaagct ggtcaccgac aagtcgccgt tccacgtcgg
      841 cgccacgcgc tccacgcgct ggatgacgcg gccggccgcg gaggaccact gggtcaggcc
      901 gcagctggtc gcggaggtca gcttcgcgga atggacgccc gatggcagca tccgccatgc
      961 gtcgttccag ggcctgcgcg aggacaaggc cgcacgggac gtcggacgcg agcgcgcgaa
     1021 ggcgccgccc acttcggaga aggccgtgac cgcgacgaag acatccagga cgcccgcggc
     1081 gcccgccgcg aagacctccg ccaaggccgg cagcgccgag gtcgagggca ttcgcatctc
     1141 gcatcccgaa cgcgtggtcg atgcggcgag cggccatacc aagctcgacc tcgcgcgcta
     1201 ctacgccagc atcgcggagt tcatgctgcc gcacctgaag tcgcggcccg tgtcgctggt
     1261 gcgcgcgccc gagggcgtcg ggcacgagct gttcttccag aagcacgccg atgtgcgcac
     1321 catgcccggc gtgaaggact tgtatggcct gtgggaaggg cacgacccgc tgatcgagat
     1381 cccgtcggcc aaggcgctga tcgccgccgc gcagatgaac gtcatcgagt tccacacctg
     1441 gaactccgtc aagcagaaga tcgacaagcc cgatcgcatg atcttcgacc tcgaccccgg
     1501 cgacggcgtg aagttcgacc aggtgcgcga gggcgcgcag ctgatgcgcg cgctgctcga
     1561 ggagctgggc ctgcgctgct ggctcaagac cagcggcggc aagggcctgc acgtggtggt
     1621 gccgatgtcg gcgcgactgg actacgacac cgtcaaggcg ttctcgcagc gcatcgtgca
     1681 gcacatggcc gcgacgatcc cgcagcgctt cgtggccaag agcggcggct cgaaccgcgt
     1741 gggcaagatc ttcatcgact acctgcgcaa cggcttcaac gcgaccacgg ccgcggcgtt
     1801 ctccgcacgg gcgcggccgg ggctcggcgt atcgatcccc atcgcgtggg acgagctggc
     1861 gcagatccgc gggggcgacc actggacgat ctcgaatgcg cgggatcgga tgtcgttcca
     1921 gaaggccgat ccgtgggcgg agtactggtc ctgccggcag acgatgaccg cggcgatgaa
     1981 ggcgctcgat gccgggtagg acaggttggc cgcaggcgtg acctgcgtcg ccggcgtgca
     2041 accgggttcc gccggacgac gcgccgggca actcccgagc gccgctcgac ggtcgcgccc
     2101 cgagaatgtc gcgcatggac ttcaaggact acgtcgcctg cgacgccacg cgcctcgccg
     2161 aactcgtcgc ccggcgcgag gtcggcgccc acgagctcct cgaactcgcg ctgacccacc
     2221 tgcgccgcac gcagccgcgc atcaacgcgg tctgccggct gatggaggcc gaggcgcgcg
     2281 cgcagctcgc cgcgccgctg cccgcgggcc cgttcgcggg cgtgcccttc ccacaggcca
     2341 tacaagtcct tcacgccggg catggtgcgc acatcggcgt gcttctggaa gaacagctcg
     2401 tgcccgacgc cctcggg
//
LOCUS       DATCRO010000769         4883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_244968, whole genome shotgun sequence.
ACCESSION   DATCRO010000769 DATCRO010000000
VERSION     DATCRO010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244968"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VIN75_20895"
     CDS             <1..506
                     /locus_tag="VIN75_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY8976685.1"
                     /translation="LLRVTIDRVPGRSYPTGESEFVLVENCEAVTRQLQYVLEVENAD
                     YSRLEVSSPGLDRPLRGERDFTRFVGEQIEITLKQPFQSRKKFVGELLAREGGWSLVL
                     PSPAVSKTQAKAGVKAVEQVLDFTLDELQESRLKPVIDFKGRRRKADLAAVPEAGDQS
                     DDGGQEE"
     gene            503..1981
                     /gene="nusA"
                     /locus_tag="VIN75_20900"
     CDS             503..1981
                     /gene="nusA"
                     /locus_tag="VIN75_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8976686.1"
                     /translation="MNREMLMLVDAISREKTVERDLVFGAVESALASATKKLHGVDVD
                     IRVAVDRETGDYETFRRWHVVPNEAGLQIPDAEILLFEAQEQIPDIEVDDYIEEPIDS
                     VPIGRIGAQAAKQVILQKIRDAEREQLLNDFLSRGERIFTGTVKRLDKGDIIVEQGRV
                     EGRLKRSEMIPKENLRSGDRVRAFIAGVDSQARGPQIMLSRSAPGFMVELFAQEVPEI
                     EQGLLEIKSCARDAGSRAKIAVLSHDKRVDPIGTCVGVRGSRVTAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVVSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLT
                     GWRINIMTAEESQAKQAEESGGVRALFVEKLDVDEEVADILIEEGFTSLEQVAYVPLQ
                     EMLEIEGFDEDTVNELRTRAKDALLTMEIAREERVEEVSQELRDLDGLTPDLIAKLAE
                     GGVHKRDDLADLAVDELVEMTGVDEAAAKALIMKAREHWFNT"
     gene            2052..4667
                     /gene="infB"
                     /locus_tag="VIN75_20905"
     CDS             2052..4667
                     /gene="infB"
                     /locus_tag="VIN75_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8976687.1"
                     /translation="MAVTTVAQFAAELNRPAATLLEQLQSAGVAKATADEALNDADKE
                     RLLDYLRNAHGTAGGERKKITLTRKSTSEIKQADASGKARTIQVEVRKKRTFVQRDDA
                     ATEEQAAAAAAAQEAELGRREEEARAQAELMRKQEEELAEKRRLREAEEARQRAEAEA
                     AAIRAMMSAPKKVLTAAKPVEAPKAADAAGIKGTIHKAKAAPGAAAPSPAGAKPGDKK
                     AVKSEKLSSSWADDSSKKRGEAGKGRGAPAGAGAAGAGRPGWRAPRGGARGRDRDSGA
                     STFQAPTEAVVQEVHVPETISVADLAHKMSVKASEVIKQMMKLGQMVTINQQLDQETA
                     MIVVEEMGHKAVTAKLDDPEAFSEEEKAAQVVGEQLPRAPVVTVMGHVDHGKTSLLDY
                     IRRARVASGEAGGITQHIGAYHVETPRGMITFLDTPGHAAFTAMRARGAKATDIVILV
                     VAADDGVMPQTKEAIHHAKAAGVPIVVAINKIDKPDANAERVKNELVAEEVVPEEFGG
                     DSPFVPVSAKTGQGVDELLEQVLLQAEVLELKAPEEAAAQGLVIEARLDKGRGPVATV
                     LVQTGTLKRGDIVLAGSSYGRVRAMIDEDGKAASEAGPSIPVEIQGLTEVPQAGDEFM
                     VLTDERRAREIATFRQGKYRDVKFARQAKVKMETLFDGMSGGDVQVLPIIIKADVQGS
                     QEALGASLLKLSTDEVKVQIVHSAVGGISESDVNLAIASKATIIGFNVRADAGARKLA
                     EGNGIDVRYYSIIYDAVDELKTAMSGMLAPEKREEVIGSAEIRTVFVASKIGTVAGSY
                     VTSGLVTRNAHFRLLRDNVVIYTGEIESLKRMKDDVREVKEGFECGIKLKNYNDIKEG
                     DQLEFFEVKEIARTL"
     gene            complement(4598..>4883)
                     /locus_tag="VIN75_20910"
     CDS             complement(4598..>4883)
                     /locus_tag="VIN75_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976688.1"
                     /translation="ARKLAENNGVDLRYYNIIYDAVDEVKAAMTGMLAPEQREEVVGA
                     AEIRQIPLDLVGDLETAVGRGRLLAHGGYSVRAISLTSKNSSWSPSLMSL"
BASE COUNT          919 a         1611 c         1710 g          643 t
ORIGIN      
        1 ggctgctgcg cgtgacgatc gatcgcgtgc cggggcgctc gtacccgacc ggcgagtcgg
       61 agttcgtgct ggtcgagaac tgcgaagctg tcacgcggca gctgcaatat gtgctcgagg
      121 tcgagaacgc ggactacagc cggctcgagg tgtcctcgcc gggcctggat cgtccgctgc
      181 ggggcgagcg cgatttcacg cgcttcgtgg gcgagcagat cgagatcacg ctgaagcagc
      241 cgttccagtc gcgcaagaag ttcgtcggcg aactcctggc gcgcgagggc ggctggagcc
      301 tggtgctgcc gtcgccggca gtcagcaaga cgcaggcgaa ggcgggcgtc aaggcggtcg
      361 agcaggtgct cgacttcacg ctcgatgagt tgcaggaatc ccggctcaag ccggtgatcg
      421 acttcaaggg gcgccgccgc aaggcggacc tcgccgccgt gcccgaggcc ggcgatcaat
      481 ccgacgacgg aggtcaagaa gaatgaaccg cgaaatgctc atgttggtgg acgcgatctc
      541 gcgcgagaag accgtcgaac gcgacctcgt gttcggcgcg gtcgaatcgg cgctcgcctc
      601 ggccaccaag aagcttcacg gtgtcgatgt ggacatccgc gtcgccgtcg accgcgagac
      661 gggcgactac gagaccttcc ggcgctggca cgtcgtgccc aacgaggcgg gcctgcagat
      721 ccccgacgcc gagatcctgc tgttcgaggc gcaggagcag atccccgaca tcgaggtcga
      781 cgactacatc gaggagccga tcgactcggt gccgatcggc cgcatcggcg cgcaggccgc
      841 caagcaggtc atcctgcaga agatccgcga cgccgagcgc gagcagctgc tcaacgactt
      901 cctgtcgcgt ggcgagcgca tcttcacggg caccgtcaag cgcctggaca agggcgacat
      961 catcgtcgag caggggcgcg tcgaaggccg actgaagcgc agcgagatga tccccaagga
     1021 gaacctgcgc tcgggcgacc gcgtgcgtgc gttcatcgcc ggggttgatt cccaggcccg
     1081 cggcccccag atcatgctgt cccgcagcgc gcccggcttc atggtggagc tgttcgcgca
     1141 ggaagtgccc gagatcgagc agggcctgct cgagatcaag agctgcgccc gcgacgccgg
     1201 ctcgcgcgcc aagatcgccg tgctgtcgca cgacaagcgc gtcgacccga tcggcacgtg
     1261 cgtgggtgtg cgcggctcgc gcgtgaccgc ggtcaccaac gagctcgccg gcgagcgcgt
     1321 cgacatcgtg ctgtggagcg aggatccggc ccagttcgtc atcggcgcgc tggccccggc
     1381 caacgtggtc tcgatcgtcg tcgatgaaga gaagcacgcg atggacgtgg tcgtggacga
     1441 ggagaacctc gccatcgcca tcggccgcgg cggccagaac gtgcgcctcg cgtcggacct
     1501 gacgggctgg cgcatcaaca tcatgacggc cgaggaatcg caggccaagc aggccgagga
     1561 atcgggcggc gtgcgcgcgc tgttcgtcga gaagctggac gtcgacgagg aagtcgccga
     1621 catcctgatc gaggaaggct tcaccagcct cgagcaggtg gcctacgtgc ccctgcagga
     1681 gatgctggag atcgaaggct tcgacgagga tacggtcaac gagctgcgca cccgggccaa
     1741 ggatgcgctg ctgacgatgg agatcgcgcg cgaggagcgc gtcgaggaag tctcgcagga
     1801 gctgcgcgac ctcgacggcc tcacgcccga cctcatcgcc aagctggccg agggcggcgt
     1861 gcacaagcgc gacgacctgg ccgacctggc ggtcgacgag ctggtggaga tgaccggcgt
     1921 cgacgaggcg gccgccaagg cgctgatcat gaaggcgcgc gagcactggt tcaatacctg
     1981 agccgcgccc gtcgcacgaa gtcaaaagtt agtacacgaa cgcccggaac accgagcaag
     2041 gaattcctga aatggcagtg accaccgtcg cccagtttgc agcggagctg aatcgccccg
     2101 ccgcaacgct gctcgaacag ctgcaatcgg caggcgtcgc caaggcgacc gccgatgagg
     2161 cgctgaacga cgcagacaag gagcggctgc tcgactactt gcgcaatgcg cacggaaccg
     2221 ccggcggcga gcgcaagaag atcacgctga cccgcaagtc gaccagcgag atcaagcagg
     2281 cggatgcgag cggcaaggcc cgcacgatcc aggtcgaggt gcgcaagaag cgcacgttcg
     2341 tccagcgcga cgatgccgcg accgaggagc aggccgctgc cgccgccgct gcgcaggagg
     2401 ccgaactggg ccgccgcgag gaagaggcgc gcgcccaggc cgagctgatg cgcaagcagg
     2461 aggaggagct cgccgagaag cgccgcctgc gcgaggccga ggaagcgcgc cagcgcgccg
     2521 aggccgaagc tgctgccatc cgcgcgatga tgtccgcgcc caagaaggtg ctgaccgcgg
     2581 ccaagccggt cgaagcgccc aaggccgccg acgccgccgg catcaagggc accatccaca
     2641 aggccaaggc cgcgccgggc gccgccgcgc cgtcgccggc cggtgccaag ccgggcgaca
     2701 agaaggccgt caagtccgag aagctgtcgt ccagctgggc cgacgattcg agcaagaagc
     2761 gcggcgaggc cggcaagggc cgtggcgctc cggccggtgc cggcgccgcg ggcgcaggcc
     2821 gtccgggctg gcgtgccccg cgcggtggcg cgcgtggccg cgaccgcgat tcgggcgcga
     2881 gcaccttcca ggcgccgacc gaggccgtcg tgcaggaagt gcacgtgccc gagaccatct
     2941 cggtggccga cctggcgcac aagatgtccg tcaaggcctc cgaagtcatc aagcagatga
     3001 tgaagctggg ccagatggtc acgatcaacc agcagctcga ccaggagacc gcgatgatcg
     3061 tggtcgagga gatgggccac aaggcggtca ccgccaagct ggacgatccg gaagccttca
     3121 gcgaggaaga gaaggccgcg caggtcgtcg gcgaacagct gccgcgcgcg ccggtcgtca
     3181 cggtcatggg ccacgtcgac cacggcaaga cctcgctgct ggactacatc cgccgcgccc
     3241 gcgtggcctc cggcgaagcc ggcggcatca cgcagcacat cggcgcgtac cacgtggaga
     3301 ccccgcgcgg catgatcacc ttcctggaca ccccgggtca cgcggccttc acggccatgc
     3361 gtgcgcgcgg cgccaaggcc accgacatcg tcatcctggt ggtggcggcc gacgacggcg
     3421 tcatgccgca gaccaaggaa gccatccacc acgcgaaggc ggccggcgtg cccatcgtcg
     3481 tggcgatcaa caagatcgac aagcccgacg ccaacgccga gcgcgtgaag aacgagctcg
     3541 tggccgaaga ggtcgtgccg gaagagttcg gcggcgactc gcccttcgtt cccgtctcgg
     3601 ccaagaccgg ccagggcgtg gacgagctgc tggaacaggt gctgctccag gccgaagtgc
     3661 tcgagctcaa ggccccggaa gaagccgcgg cccagggcct ggtcatcgaa gcgcgcctcg
     3721 acaagggccg cggcccggtc gccaccgtgc tggtgcagac gggtacgctc aagcgcggcg
     3781 acatcgtgct ggccggctcg agctacggcc gtgtccgcgc gatgatcgac gaggacggca
     3841 aggcggcgag cgaggccggc ccgtcgatcc cggtcgagat ccagggcctg accgaggtgc
     3901 cgcaggccgg cgacgagttc atggtgctga ccgacgagcg ccgtgcgcga gagatcgcca
     3961 cgttccgcca gggcaagtac cgcgacgtca agttcgcgcg ccaggccaag gtcaagatgg
     4021 agacgctgtt cgacggcatg tccggcggcg acgtccaggt gctgccgatc atcatcaagg
     4081 ccgacgtgca gggctcgcag gaagcgctcg gcgcctcgct gctcaagctg tccacggacg
     4141 aggtcaaggt gcagatcgtg cactcggcgg tcggcggcat cagcgagtcc gacgtcaacc
     4201 tggcgatcgc ctcgaaggcc accatcatcg gcttcaacgt ccgggccgac gccggcgcgc
     4261 gcaagcttgc cgagggcaac ggcatcgacg tccgctacta cagcatcatc tacgacgccg
     4321 tggacgagct gaagacggcc atgtcgggca tgctggcgcc ggaaaagcgc gaagaggtca
     4381 tcggctcggc cgagatccgc accgtgttcg tggcgtccaa gatcggcacc gtcgccggct
     4441 cgtacgtcac ctcgggcctg gtcacgcgca acgcgcactt ccgcctgctg cgcgacaacg
     4501 tggtgatcta caccggcgag atcgagtcgc tcaagcggat gaaggacgac gtgcgcgagg
     4561 tcaaggaagg cttcgagtgc ggtatcaagc tgaagaacta caacgacatc aaggaagggg
     4621 accagctgga attcttcgag gtcaaggaaa tcgcccgcac gctgtaacct ccatgcgcaa
     4681 gaagtcgtcc acgcccaacc gcggtttcca gatcgccgac cagatccaga gggatctggc
     4741 ggatctccgc ggcacccacg acttcctcgc gctgttccgg cgccagcatg cccgtcatcg
     4801 ccgccttcac ctcgtccacg gcgtcgtaga tgatgttgta gtagcgcagg tcgacgccgt
     4861 tgttctcggc gagcttgcgc gcg
//
LOCUS       DATCRO010000770         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_255940, whole genome shotgun sequence.
ACCESSION   DATCRO010000770 DATCRO010000000
VERSION     DATCRO010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255940"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1892)
                     /locus_tag="VIN75_20915"
     CDS             complement(102..1892)
                     /locus_tag="VIN75_20915"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY8976689.1"
                     /translation="MAPAQLDAPVDAGLAPHRFEIPGALADLATARLTPARLASALRA
                     RAAAAGAGPSAPALAATVYSPAQIRAAYGLPPLSGATPADLGAGQTIYIVDAHDHPNA
                     FADLAAFSTTFGLPGCASAPLSAPAGAGCTFSVAYTDHGGQLLKSAPAYDAEWAAEIA
                     LDVEWAHAIAPLARLVLIEVPGDDSTQLLGGVALANRMGPGVVSMSFGAPEGPWARQT
                     DASFSAPGMTYVAAAGDAGAQVLWPAVSPKVLAVGGTSLQWDGLQARRESAWAKSGGG
                     VSAVEPLPAWQSGVAVPGVGAATMRAVADVAFNADPTTGQYVALSARGAARTAWNSYG
                     GTSIGAPQWAGLVAVANARRAAIGKKPLGDFHAALYQAIANSPVPHAASFADVVDGRD
                     GACAACVAAIGYDTLTGWGTPNADVLLPALASAAADGVAPVVPGGAFRARTDGAFAAS
                     LAIVAPAGVTTSYALSGAPEGLAVDAAGVLRWAAPVAGSYGFTATATTSLGLSASARY
                     TLSVVKVNHAPTLASGIVAVPPGVPLRLALSGVDVDGDALTYTMTGAPRGLALSSAGV
                     LSWPLTQRGRWVLHVVARDAQGLASAPGAR"
     gene            2246..2905
                     /locus_tag="VIN75_20920"
     CDS             2246..2905
                     /locus_tag="VIN75_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8976690.1"
                     /translation="MYTLYGFKGSGSASVECALEMTGAPYRIVEAASWENNDALAELE
                     RVNPLRQIPTLQLPDGSILTESAAILAHLAFAFPGAGLMPPDPAGRDQSLRGMVYIAA
                     NCYPCITILDYPERFTTATDDAAKAAIRDGTRARLHHHWDLFADQFKPADDRFLAGDQ
                     PGALDLYAAVVSHWGKARPHARESRPAFAALLERIDSHPAVALVFGRHWPTAAELPTG
                     A"
     gene            complement(2959..3306)
                     /locus_tag="VIN75_20925"
     CDS             complement(2959..3306)
                     /locus_tag="VIN75_20925"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8976691.1"
                     /translation="MSNTIDLKVPGTRARIPVRTTGQWRSRFLGLKGAPELKKSRGVW
                     VKRCRSINTFGFSQAIDVVFLSADGVVTKVVSGLKPWRMSSCPDARTVLELRAGLASR
                     MNLAPGVEMGLLA"
     gene            3646..4257
                     /locus_tag="VIN75_20930"
     CDS             3646..4257
                     /locus_tag="VIN75_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8976692.1"
                     /translation="MALDTWLLFLAASIGISLTPGPNGLLALTHGALHGSRRTLFTIA
                     GGVLGFVAVIALCMFGIGALVKSSIVWLTVLKWVGGLYLAYLGVKLWRSPPVAVQVDE
                     GGARASNGSLFRAGLLAAGTNPKALLFFSALLPQFLDPHRSLELQLVVVAATFGTTEF
                     LTEYALARTAHRIRPWLARVGRRFNRVCGGLFVAVGAALPLRA"
     gene            4302..4961
                     /locus_tag="VIN75_20935"
     CDS             4302..4961
                     /locus_tag="VIN75_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8976693.1"
                     /translation="MALQLFFHPFSSYSQKALIALYENATPFTPRTLSPDDPATGAEF
                     AGHWPIRRFPILVDGDRTILEATTVIEYLDVAHPGPVRLIPQDPQAAIDVRMMDRVFD
                     NYVSTPQQRVVYDALRPAQVRDAHGVAEARAMLEQAYAWLDARMATREWAAAGQFSLA
                     DCAAAPSLFYADWTHPIDARFEHLRAYRARLLARPSFARAVDEARPFRPYFPLGAPDR
                     D"
     gene            complement(4978..5583)
                     /locus_tag="VIN75_20940"
     CDS             complement(4978..5583)
                     /locus_tag="VIN75_20940"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY8976694.1"
                     /translation="MTTPPWKFSLEPARDKPTLRRQLKAERLAMPDRHQRSVHMQEVL
                     RVWLVTRPETSIGAYWPIKGEFDAMPALFRWTEGEPYRLIGLPVIDRETKQLDFHVWY
                     PGCPMEDDAYGIPKPKGTDKFEPELLLVPCVGFGDNGVRLGYGGGFYDRTLAALSPRP
                     YTVGIGYSHGYVPWLESEPHDMPLDAMLTEDGVFWERERDT"
     gene            5683..6252
                     /locus_tag="VIN75_20945"
     CDS             5683..6252
                     /locus_tag="VIN75_20945"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8976695.1"
                     /translation="MNDAGFVSHCLELIAPLGRTSSRRMFSGHALYIDGLCMALIIRD
                     TLYLKVDDTSRPLAERAGCKPFTYETRKGERAALGYYTAPEESMESPAEMLPWARRAL
                     AAAVAARAKAPAKKKPAAKAPAKEATARSAVAKKAPAKVAAKKAAPAKAAAAKKVAAK
                     KSPTRKAAAKTAVARKTPAKVAAKKAARA"
     gene            6249..>6768
                     /locus_tag="VIN75_20950"
     CDS             6249..>6768
                     /locus_tag="VIN75_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976696.1"
                     /translation="MNIGRQRPLVLDHLRSFEAVARRAGFGAAAQELHVTQSAVSRQI
                     KSLEDELGAPLFTRGTRRVELTQAGQLLLRAITPALGRIDSAVRQIRQLRGRAQVSLS
                     TFPSFASLWLLPRLPTFEKGQPAIDIRISAVDRLVELDDPDHDLVLRQCRPEDAPPGA
                     IRMFGEVCTPVIG"
BASE COUNT          956 a         2462 c         2445 g          905 t
ORIGIN      
        1 taccgccagg acggcaagac gtccggcccc gtgaacccgc tggacggcat cgcgttccag
       61 cgcctgtggg aatcgcgcgc ctacgagctc ggcggcggcg gctacctcgc gcccggcgcg
      121 ctcgccaggc cctgcgcgtc gcgcgccacc acgtgcagca cccagcgtcc gcgctgcgtc
      181 agcggccagc tcagcacgcc ggcgctcgac agcgccaggc cgcgcggcgc gccggtcatc
      241 gtgtaggtca gcgcgtcgcc gtcgacgtcg acgccggaca acgcgagccg cagcggcacg
      301 ccgggcggca cggccacgat gcccgaggcc agcgtcggcg catggttcac cttgacgacc
      361 gacagcgtgt agcgcgcgct ggccgacagc cccaggctcg tcgtggcggt cgccgtgaac
      421 ccgtagctgc cggcgacggg cgccgcccag cgcagcacgc cggcggcatc gaccgcgaga
      481 ccctcgggcg cgccggacaa cgcgtagctc gtggtcacgc ccgcgggcgc cacgatggcc
      541 aacgacgccg cgaacgcgcc gtccgtgcgg gcgcggaatg cgccgcccgg cacgacgggc
      601 gcgacgccgt ccgccgcggc gctggccagc gcgggcagca gcacgtcggc gttgggcgtg
      661 ccccagccgg tgagggtgtc gtagccgatg gccgccacgc aggcggcgca ggcgccgtcg
      721 cggccgtcga cgacgtcggc gaatgacgcg gcgtgcggca ccggcgagtt ggcgatcgcc
      781 tggtacagcg ccgcgtggaa atcgccgagc ggcttcttgc cgatcgccgc gcgacgcgcg
      841 ttggcgacgg ccaccagccc ggcccactgc ggcgcgccga tgctggtgcc gccgtacgaa
      901 ttccaggcgg tccgggccgc gcccctggcg ctcagcgcga cgtactggcc ggtggtcggg
      961 tcggcgttga acgcgacgtc ggcgaccgcg cgcatggtcg cggcgcccac gcccggcacc
     1021 gccacgcccg actgccacgc cggcagcggc tcgaccgcac tgacgccgcc gcccgacttg
     1081 gcccacgccg actcgcggcg cgcctggagg ccgtcccact gcaggctggt gccgcccacc
     1141 gcgagcacct tgggcgacac cgccggccac aacacctgtg cgccggcgtc gcccgccgcc
     1201 gcgacgtagg tcatgccggg cgcggagaag ctcgcgtcgg tctgcctggc ccacgggccc
     1261 tcgggcgcgc cgaagctcat cgacaccacg cccggcccca tccggttggc cagcgcgacg
     1321 ccgccgagca gttgcgtgct gtcgtcgccg ggcacctcga tcagcacgag ccgcgcgagc
     1381 ggcgcgatcg catgcgccca ttcgacgtcg agcgcgatct ccgcggccca ctcggcgtcg
     1441 taggccggcg cggacttcag caactggccg ccgtgatcgg tgtaggccac ggagaacgtg
     1501 cagccggcgc cggccggggc cgacagcggc gcgctggcgc agccgggcag gccgaaggtc
     1561 gtcgagaacg ccgcgagatc ggcgaaggcg ttcgggtggt cgtgggcgtc gacgatgtag
     1621 atggtctggc cggcgccgag gtcggccggc gtggcgccgg acagtggcgg caggccgtac
     1681 gcggcgcgga tctgcgcggg cgagtagacg gtggcggcca gggccggcgc ggacgggccg
     1741 gcgcccgccg cggccgcacg cgcgcgcagc gcgctcgcca ggcgcgcggg cgtcaggcgc
     1801 gcggtcgcaa ggtccgcgag cgcgccgggg atctcgaagc ggtgcggcgc gaggcccgcg
     1861 tccacgggcg cgtccagctg cgccggggcc atgtggaagg cggggtgcgc atggacgccg
     1921 gcgccgtcgt cgatcgcggc ctcgggcgcg gcgtcgctgc cgccgccgca ggccgtcaac
     1981 gcggcagcga gcaggctggt gaagaaaaag aggcggacgg ggcggcgatg cgacgacaag
     2041 ggcgatcctg ggcgagaaac caatccccgg atctatcggc accgccgccc cgcttccgga
     2101 gggcggatcg tgcacgtcgc atcaatcggt cacatccccg ccgctgtcgg gggcgctggc
     2161 gtgacttccg tcatccggcg ggctgcgtgt caagatcgcc gcacgcgcaa cgacgccgcg
     2221 ccgaccagcg cgacaggaga ccggcatgta cacgctgtac ggattcaagg gctcgggatc
     2281 ggcctcggtg gaatgcgcgc tcgagatgac gggcgcgccg taccgcatcg tcgaggccgc
     2341 gtcctgggag aacaacgacg cgctcgccga gctcgagcgc gtgaatccgc tcaggcagat
     2401 cccgacgctg cagctgcccg acggttccat cctgaccgag agcgccgcca tcctcgcgca
     2461 cctggccttc gcgttcccgg gcgccggcct gatgccgccc gacccggcgg gccgcgacca
     2521 gtcgctgcgc ggcatggtgt acatcgccgc gaactgctac ccgtgcatca cgatcctgga
     2581 ctatcccgag cgcttcacca ccgccacgga cgacgcggcc aaggccgcga tccgcgacgg
     2641 cacgcgcgcg cggctgcatc accactggga cctgttcgcg gaccagttca agccggccga
     2701 cgaccgattc ctggccggcg accagcccgg cgcgctcgac ctgtatgcag ccgtcgtctc
     2761 ccactggggc aaggccaggc cgcatgcgcg cgaaagccgc cccgcgttcg cggcgctgct
     2821 cgagcgcatc gactcgcatc cggcggtggc gctggtcttc ggccgacact ggcccacggc
     2881 cgcggagctg ccgaccggcg cctgaaatgc aaaacggcga cgcgggtggc gtcgccgttc
     2941 gtgcgggagg ccgcggactc aggccagcag gcccatctcc acgccggggg cgagattcat
     3001 gcggctggcc aggccggcgc gcagctcgag cacggtgcgg gcgtcggggc agctggacat
     3061 gcgccagggc ttcaggccgg acaccacctt ggtgacgacg ccgtcggcgc tcaggaacac
     3121 gacatcgatc gcctgcgaaa agccgaaggt gttgatcgag cggcaacgct tgacccacac
     3181 gccacgcgac ttcttcagct ccggcgcgcc cttcaggccc aggaaacggc tgcgccattg
     3241 gccggtggtg cggacgggga tgcgagcgcg cgttccgggg accttgagat cgatcgtgtt
     3301 cgacatgacg gatatcctgc tgcctcgatg cttccggcgg gtggcggtca tgccgcgtgg
     3361 gccggaatcc cattcgtgtt cgttgaccag aagcatacct gtcgggcgtg ccgaaaatca
     3421 cggcgattcg ggtaaacgac gacacccgat tcaccctgtt acgggctaac ggtgacaagg
     3481 tgcttcggcg cttgacaaga cgtcaggact gttcggattc gatgcgggat tccacctcgc
     3541 cgacgcggtc ggcatcgatt cgatcgcccc ggccggcagc acagccacgg ccatcggcga
     3601 cactcggcga gacgcgccgg ccgcccgccg gccaaggatc gctccatggc cctcgacacc
     3661 tggctcctgt tcctcgccgc ctccatcggc atctcgctga cgccgggccc caacgggctg
     3721 ctcgcgctga cgcacggcgc gctgcacggc agccggcgca cgctgttcac gatcgccggc
     3781 ggcgtgctgg gtttcgtggc ggtgatcgcg ctgtgcatgt tcggcatcgg cgcgctggtc
     3841 aagtcgtcga tcgtctggct gaccgtgctc aagtgggtcg gcgggctgta cttggcctac
     3901 ctgggcgtca agctgtggcg ctccccgccg gtggccgtgc aggtggacga gggcggggcg
     3961 cgggccagca acggctcgct gttccgcgcc ggcctgctgg ccgcgggcac caacccgaag
     4021 gcgctgctgt tcttcagcgc gctgctgccg cagttcctcg acccgcaccg cagcctcgag
     4081 ctgcagctcg tggtcgtggc cgcgaccttc ggcacgaccg agttcctgac cgagtacgcg
     4141 ctggcccgca cggcccaccg catccggccc tggctcgcgc gcgtgggccg gcgcttcaac
     4201 cgcgtctgcg gcggcctgtt cgtcgccgtg ggcgccgcgc tgccgctgcg cgcctgatat
     4261 cgccttgccc cgccaccctc gccagacgac cggagaccgc catggccctg caactgttct
     4321 tccacccgtt ctcgtcgtac tcgcagaagg cgctgatcgc gctgtacgag aacgccacgc
     4381 cgttcacgcc gcgcacgctg tcgcccgacg acccggccac cggcgccgag ttcgcgggcc
     4441 actggccgat ccggcgcttc ccgatcctgg tcgacggcga tcgcacgatc ctggaggcca
     4501 cgaccgtcat cgagtacctg gacgtcgcgc atcccgggcc ggtgcgcctg atcccgcagg
     4561 acccgcaggc cgcgatcgac gtgcgcatga tggatcgcgt gttcgacaac tacgtctcga
     4621 cgccgcagca gcgcgtggtc tacgacgcgc tgcggccggc gcaggtgcgc gacgcgcacg
     4681 gcgtggccga ggccagggcg atgctcgagc aggcctacgc gtggctggac gcgcgcatgg
     4741 ccacgcgcga gtgggccgcc gccggccagt tcagcctggc cgattgcgcg gccgcgccgt
     4801 cgctgttcta cgcggactgg acgcatccga tcgacgcgcg gttcgagcac ctgcgtgcgt
     4861 atcgcgcacg cctgctggca cggccgtcgt tcgcacgggc ggtggacgag gcgcggccgt
     4921 tcaggccgta cttcccgctg ggggcgccgg atcgggactg atcagccgat cggcaggtca
     4981 ggtgtcgcgc tcgcgttccc agaacacgcc gtcctcggtg agcatggcgt ccagcggcat
     5041 gtcgtgcggc tcggactcga gccagggcac atacccgtgg ctgtatccga tgccgaccgt
     5101 gtacggccgc ggcgacagcg ccgcgagggt gcggtcgtag aaaccgccgc cgtagcccag
     5161 gcgcacgccg ttgtcgccga agcccacgca gggcaccagc agcagctcgg gctcgaactt
     5221 gtcggtgccc ttgggcttgg ggatgccgta ggcgtcgtcc tccatcgggc agcccgggta
     5281 ccagacgtgg aagtcgagtt gcttggtctc gcggtcgatc acgggcaggc cgatcagccg
     5341 gtacggctcg ccctccgtcc agcggaacag cgcgggcatc gcgtcgaact cgcccttgat
     5401 gggccagtag gcgccgatgg aggtctcggg ccgcgtgacc agccacacgc gcagcacttc
     5461 ctgcatgtgc acggagcgct ggtggcgatc gggcatcgcg agccgctcgg ccttgagctg
     5521 gcggcgcagc gtgggcttgt cgcgggcggg ctcgagcgag aacttccagg gcggcgtggt
     5581 catgctccgg attgtggagg ggccttcagc ttcctgcaaa tccgcccctt aaactgcagt
     5641 cgagacgctc tgctcgacag tgcgcgcaac aaaggtcgat cgatgaacga tgccggattc
     5701 gtgagccatt gcctggaact gatcgccccg ctgggccgca cgtcgtccag gcgcatgttc
     5761 agcggccatg cgctttatat cgacggcctg tgcatggcgc tgatcatccg cgacacgctg
     5821 tacctgaagg tcgacgacac gagccgtccg ctggccgagc gcgcgggctg caagccgttc
     5881 acctacgaga ccaggaaggg cgagcgggcc gctctcggct actacaccgc gcccgaggaa
     5941 tcgatggagt cgccggcgga gatgctgccc tgggcgcgcc gcgcgctggc cgcggcggtg
     6001 gcggcgcgcg cgaaggcgcc cgcgaagaag aagccggccg ccaaggcgcc ggcgaaggag
     6061 gcaaccgcca ggtcggcggt cgcgaagaag gcgccggcca aggtcgctgc gaagaaggcc
     6121 gcacccgcga aggcggccgc tgccaagaag gttgcggcga agaagtcacc cacccgcaag
     6181 gctgccgcga agaccgcggt cgccaggaag acgccggcca aggttgcggc gaagaaggcg
     6241 gcgcgcgcat gaacatcggt cgccagcggc cgctggtcct cgaccacctg cgcagcttcg
     6301 aggccgtcgc gcgacgggcc ggcttcggcg cggcggcgca ggagctgcac gtgacgcagt
     6361 cggccgtcag ccgccagatc aagagcctgg aggacgagct cggcgcgccg ctgttcacgc
     6421 gcggcacgcg ccgcgtcgaa ctcacgcagg ccggccagct gctgctgcgc gcgatcacgc
     6481 cggcgctcgg gcgcatcgac agcgccgtgc gccagatccg ccagctgcgc ggccgcgcgc
     6541 aggtgagcct gtccacgttc ccgtcgttcg cgtcgctgtg gctgctgccg cgcctgccca
     6601 ccttcgagaa gggccagccg gcgatcgaca tccgcatctc ggccgtcgac cgcctcgtcg
     6661 agctcgacga ccccgaccac gacctcgtgc tgcgccagtg ccgccccgag gacgccccgc
     6721 ccggcgcgat ccgcatgttc ggcgaggtct gcacgccggt gatcggcg
//
LOCUS       DATCRO010000771         5961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_976218, whole genome shotgun sequence.
ACCESSION   DATCRO010000771 DATCRO010000000
VERSION     DATCRO010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976218"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /locus_tag="VIN75_20955"
     CDS             complement(<1..2192)
                     /locus_tag="VIN75_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8976697.1"
                     /translation="MRGVIGRWCGAAWLVASVVLVSLAGCDNSPWESGAASKNTLFTA
                     MQESSPRHMDPTASYWSNDTTFTYQIYEPPYGYHYLKRPYQLIGKAAEEVARPRFYDK
                     DGKQLPEDAPADQVAESVYDVKIKHGILFQPHPAFAKDAKGHYWYHDMKPGELGNRTS
                     PMQFEHQGTRELVADDYVYALKRHATTRITTPIFGIFSEYVVGLKDYGELIKKEDAKL
                     RAKLDPASVDLPFLDFRKYPLAGASAPDKYTFRVHIKGRYPQWNYWMAMTFMAPVPWE
                     ADAFYAQPGMAAASLTLDTWPVGTGPFMQTEYFKDRRQVMVRNPNYRGEPYPCEGMPG
                     DKEEGLLDDCGKKTPFVDKIVVTAEREKVPQKTKFLQGYYDLEVFERTDLGMEYIVAK
                     QDSEDIRKEYDAKGFRLDRFDDVWKYNVGYNMLDPVLGKGDTPEQQEKNRKLRQAINI
                     AIDWEEYSRIFPNKAGVTAQTPLPNGIFGSRQSTPEGVNPYTHRLVNGLPVRRSIEEA
                     RKLMAEAGYPDGRDVRTGRPLVLNYDFYALPTPERKVEIDWMVRRFAKLDIQLEVRAT
                     DNNQFQDKVRKGKHQVFWYGWNADYPDAENFLFLFYGPNAKSVSDGENTTNYQNPEYD
                     RLFEQMKTLDDGPAKQALIDKMVKILEVDAPETFGFFPYSSAAVQHWVYNSKPAILIR
                     DHGRYLRLDVDERVRRQAEWNKPVWWPMILLTLALVGLLLLAARSFKRR"
     gene            complement(2462..3415)
                     /locus_tag="VIN75_20960"
     CDS             complement(2462..3415)
                     /locus_tag="VIN75_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY8976698.1"
                     /translation="MTSLTIYTPAGVLAKAAPLKLAARRLGKLGFDVSIDDAALAKHQ
                     RFGGDDDLRLDAIHRVARARPGVALASRGGYGLTRLLDRLDWKLLAKSVESGTRWVGY
                     SDLTALQMGLLAHAKAPTWWGPLACDDFGRDELDEVTPDCFAEAMSGALEAVGFRTEA
                     GFDGLDLRGPTWGGNLAMLCSLLGTPHWPKVRGGVLMLEDVAEHPYRVERMLLQLHQA
                     GVLASQKAILLAEFSDWKKSPLDRGFNLKTVVAHLRSLVDVPILTGFPFGHVPTKVTV
                     PFGVKAHLAVQGRDAFLGWQEGRAHHHHDDGHDHAGHDHEH"
     gene            complement(3412..4041)
                     /gene="tadA"
                     /locus_tag="VIN75_20965"
     CDS             complement(3412..4041)
                     /gene="tadA"
                     /locus_tag="VIN75_20965"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8976699.1"
                     /translation="MFTPAEERAMRQALDQAQNAWLVGEVPVGAVILRDDADGLPQVI
                     ATGYNRPITTTDPTAHAELVALRHAAQLLDNYRLPDCTLVVTLEPCAMCAMALMHARF
                     KRVVFGARDPKTGAAGSVVDLFALPQLNHHTAIVGGCMETECGTMLRDFFAERRAAHK
                     ARQAARRVPAEDDAIPVADVTVDDPIPVGTAVEIDPDSPVPPDARGAPA"
     gene            complement(4095..4349)
                     /locus_tag="VIN75_20970"
     CDS             complement(4095..4349)
                     /locus_tag="VIN75_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976700.1"
                     /translation="MRAFMTLGSLLIVLAIIALSVRSQLQANKRFLPTSAAASGVASM
                     PLEGTPKQVMSQYQRELDQAMKADAQHTADQAASAADQAR"
     gene            4499..5257
                     /locus_tag="VIN75_20975"
     CDS             4499..5257
                     /locus_tag="VIN75_20975"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEY8976701.1"
                     /translation="MCSNYRPVTRMDRLLTFFGVERGKDEDPHDVFPTDMAPFIRLSI
                     EGQEGGRPALVAEDGTFGLLPHFATEVSFGRRTYNARSETIHSLASFRHAWAAGQRCI
                     IPAEAIYEPCYESGKAVRWRISQEGDVPMGIAGLYRRWRNPKGGELFTFAMVTVNADA
                     HPLYRRMFKPGDEKRMVVILDPEEYLPWLTCGEIEAKRYFRPWPGILLGEPAPLARPP
                     KVLADPPPKDEIKPPKAPKPTPPPSPPPAQGDLF"
     gene            complement(5323..>5961)
                     /locus_tag="VIN75_20980"
     CDS             complement(5323..>5961)
                     /locus_tag="VIN75_20980"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY8976702.1"
                     /translation="ASRIAMLRQCSLFDEPPAGLGMGPFVRPPRLAIVPGRVHSVFYA
                     LVPSGADAIRLHAHARRGEEGLGFAGKATDAHRLHVTLHALGAHAVEPPESEVDRWCR
                     AAAAVRQAPFDIVFDRVCTFGGKDNAWVFTSSDDAALLALHEALGIALANTGERVVRQ
                     RIAPHMTMSWRGRRIPEAAIEPVRWRAADLVLIDSHHGEGEHQVLGRWALSG"
BASE COUNT          854 a         2050 c         2082 g          975 t
ORIGIN      
        1 cgccgcttga agctgcgcgc ggccagcagc agcaggccca cgagcgccag cgtcagcagg
       61 atcatcggcc accagaccgg cttgttccac tcggcctggc ggcgcacgcg ctcgtccacg
      121 tccaggcgca ggtagcggcc gtggtcgcgg atgaggatgg ccggcttgga gttgtagacc
      181 cagtgctgca ccgcggccga cgagtacggg aagaagccga aggtctcggg cgcgtcgact
      241 tccaggatct tgaccatctt gtcgatcagc gcctgcttgg cgggaccgtc gtcgagcgtc
      301 ttcatctgct cgaacaggcg gtcgtactcg ggattctggt agttggtggt gttctcgccg
      361 tcggacacgc tcttggcgtt cggcccgtag aacaggaaca ggaagttctc ggcgtcggga
      421 tagtcggcgt tccagccgta ccagaacacc tggtgcttgc ccttgcgcac cttgtcctgg
      481 aactggttgt tgtcggtggc gcgcacctcg agctggatgt cgagcttggc gaaccggcgc
      541 accatccagt cgatctcgac cttgcgctcg ggcgtgggca gcgcgtagaa gtcgtagttc
      601 agcaccagcg ggcggccggt gcgcacgtcg cggccgtccg ggtagccggc ctcggccatc
      661 agcttcctgg cttcctcgat cgagcggcgc acgggcaggc cgttgaccag gcggtgcgtg
      721 tacggattga cgccttcggg cgtggactgg cgcgatccga agatgccgtt gggcaacggc
      781 gtctgcgccg ttacgccggc cttgttcggg aagatgcgcg agtactcctc ccagtcgatc
      841 gcgatgttga tcgcctggcg cagcttgcgg ttcttctcct gctgctcggg cgtgtcgccc
      901 ttgcccagca ccgggtcgag catgttgtag ccgacgttgt acttccagac gtcgtcgaaa
      961 cggtcgaggc ggaatccctt ggcgtcgtat tccttgcgga tgtcctcgga gtcctgcttg
     1021 gcgacgatgt actccatgcc caggtcggtg cgctcgaaca cctcgaggtc gtagtagccc
     1081 tgcaggaact tggtcttctg cggcaccttc tcgcgctcgg cggtgacgac gatcttgtcg
     1141 acgaacggcg tcttcttgcc gcagtcgtcg agcaggccct cctccttgtc gccgggcatg
     1201 ccctcgcatg ggtagggctc gccgcggtag ttcgggttgc gcaccatgac ctggcggcga
     1261 tccttgaagt actcggtctg catgaacggg ccggtgccca cgggccaggt gtccagcgtg
     1321 aggctggccg cggccatgcc gggctgggcg tagaacgcgt cggcctccca gggcacgggc
     1381 gccatgaagg tcatcgccat ccagtagttc cactgcggat agcggccctt gatgtgcacg
     1441 cggaacgtgt acttgtcggg cgcgctggcg ccggccagcg ggtacttgcg gaagtccagg
     1501 aacggcaggt ccacgctggc cggatcgagc ttggcgcgca gcttcgcgtc ctccttcttg
     1561 atcagctcgc cgtagtcctt caggcccacc acgtactccg agaagatgcc gaagatcggc
     1621 gtcgtgatgc gcgtggtggc gtgccgcttg agcgcgtaga cgtagtcgtc ggcgacgagc
     1681 tcgcgcgtgc cctggtgctc gaactgcatc ggcgacgtgc ggttgccgag ctcgcccggc
     1741 ttcatgtcgt ggtaccagta gtgccccttc gcgtccttgg cgaaggccgg gtgcggctgg
     1801 aacaggatgc cgtgcttgat cttgacgtcg tagacgctct cggccacctg gtcggcgggc
     1861 gcgtcctcgg gcagctgctt gccgtccttg tcgtagaagc gcggcctggc cacctcctcg
     1921 gccgccttgc cgatcagctg gtacgggcgc ttgaggtagt ggtagccgta gggcggctcg
     1981 tagatctggt acgtgaacgt cgtgtcgttg gaccagtacg atgccgtcgg atccatgtgg
     2041 cgcggcgagc tctcctgcat cgcggtgaac agcgtgttct tgctggccgc gccgctttcc
     2101 caggggctgt tgtcgcaacc ggccagggac accagcacca cgctcgccac cagccacgcg
     2161 gcgccgcacc aacgaccaat cacaccgcgc atgcggatct ccagaactgc ggtggcgaag
     2221 cccggctcgg ggtgacctcg ccggcttgcc atcaaaacaa aaccgcctcc ggcggccgcc
     2281 gggtcgtgcc ctgggccgcg cgaggcgttt gctgcgattt taggggtgcc cccaccccgg
     2341 ccccctaggg gttttcagct cttgctgcgc cgcagcaagc aaggtccggg cccatttttt
     2401 ggcgccgcgg tcgccagcct gcctagacag gttcggacaa gccggcccgc gggacgtgtg
     2461 gtcagtgctc gtgatcgtgg cccgcgtggt catggccgtc gtcgtggtga tggtgcgcgc
     2521 gcccttcctg ccagcccagg aacgcgtcgc gcccctgcac cgccaggtgc gccttcacgc
     2581 cgaacggcac ggtcaccttg gtgggcacgt ggccgaacgg aaagcccgtg aggatgggca
     2641 cgtccaccag cgagcgcagg tgcgccacca ccgtcttcag gttgaagccg cgatccagcg
     2701 gcgacttctt ccagtcgctg aactcggcca gcaggatcgc cttctgcgac gccagcacgc
     2761 cggcctggtg cagctgcagc agcatgcgct cgacgcggta cgggtgctcg gccacgtcct
     2821 ccagcatcag caccccgccc ctgaccttgg gccaatgcgg cgtgcccagc agcgagcaca
     2881 gcatcgcgag gttgccgccc caggtcgggc cgcgcaggtc cagcccgtcg aagccggcct
     2941 cggtgcggaa gccgacggcc tccagcgcgc cggacatcgc ctcggcgaag caatcgggcg
     3001 tgacctcgtc gagttcgtcc cggccgaagt cgtcgcaggc gagcggcccc caccaggtgg
     3061 gcgccttggc gtgcgcgagc aggcccatct gcagcgccgt caggtcgctg tagccgaccc
     3121 agcgcgttcc ggactcgacg ctcttggcga gcagcttcca gtcgagccgg tcgagcaggc
     3181 gcgtcaggcc atagccgccg cgcgacgcga gcgcgacgcc cggacgcgcg cgcgcgacgc
     3241 ggtggatggc gtccaggcgc aggtcgtcgt cgccgccgaa gcgctggtgc ttggcgagcg
     3301 cggcgtcgtc gatcgagacg tcgaagccga gcttgccgag gcgcctcgcg gcgagcttca
     3361 gcggcgcggc cttggcgagc acgccggcgg gggtgtagat ggtcagcgac gtcatgcggg
     3421 cgctcctcga gcgtctggcg gaacagggga atcgggatcg atctcgacgg ccgtgccgac
     3481 cgggatcggg tcgtcgacgg tgacgtcggc gacggggatg gcgtcgtcct cggccggcac
     3541 gcggcgcgcg gcctggcgcg ccttgtgcgc ggcgcggcgc tcggcgaaga agtcgcgcag
     3601 catcgtgccg cactcggtct ccatgcagcc gccgacaatg gcggtgtggt ggttgagctg
     3661 gggcaacgcg aacaggtcga cgacggagcc cgccgcgccc gtcttcgggt cgcgcgcgcc
     3721 gaacacgacg cgcttgaagc gcgcgtgcat cagcgccatc gcgcacatcg cgcacggctc
     3781 cagcgtcacg accagcgtgc aatcgggcag gcggtagttg tccagcagct gcgccgcgtg
     3841 gcgcaacgcc acgagctcgg cgtgggcggt ggggtcggtg gtggtgatgg gccggttgta
     3901 gccggtggcg atcacctgcg gcaggccgtc ggcgtcgtcg cgcaggatca cggcgcccac
     3961 gggcacctcg cccaccagcc atgcgttctg cgcctggtcg agggcctggc gcatcgcgcg
     4021 ctcctcggcc ggcgtgaaca tgggcgccgg acccgggtcg gtttgggatt cggtctgcaa
     4081 gacgatgggg tcgatcaacg cgcctggtcg gcggcgctgg ccgcctggtc ggccgtgtgc
     4141 tgggcatcgg ccttcatggc ctggtcaagc tcgcgctggt actgcgacat cacctgcttg
     4201 ggcgtgccct ccagcggcat cgacgcgacg ccggacgccg cggccgacgt cggcaggaat
     4261 cgcttgttcg cctgcagctg gctgcgcacg gacagggcga tgatggccag aacgatcagc
     4321 agcgatccga gcgtcatgaa ggctctcatg tcgatgtggg cggatgggaa ggcgaaacgg
     4381 gcattatcgt cgctggatgc gcgccgccgc ccgtcgcagg gcgcgtggcg acgagcggcc
     4441 gcgcgaagcg gacacgcggt cgcacggtcg cacgcttggg ccgcgctagc atcgctgcat
     4501 gtgctcgaac taccgccccg tcacccgcat ggatcgcctg ctgacgttct tcggcgtcga
     4561 gcgcggcaag gacgaggatc cgcacgacgt ctttcccacc gacatggcgc cgttcatccg
     4621 gctgtcgatc gagggccagg aaggcggccg tccggccctg gtggccgagg acggcacctt
     4681 cggcctgctg ccccacttcg cgaccgaggt cagcttcggg cgccgcacct acaacgcgcg
     4741 cagcgagacg atccactcgc tcgcgagttt ccgccacgcc tgggccgccg gccagcgctg
     4801 catcattccg gccgaggcga tctacgagcc ctgctacgag agcggcaagg ccgtgcgctg
     4861 gcgcatctcg caggaaggcg acgtgccgat gggcatcgcc ggcctctatc gccgctggcg
     4921 caaccccaag ggcggcgagc tgttcacctt cgcgatggtg accgtcaacg ccgatgcgca
     4981 cccgctatac cgccgcatgt tcaagccggg cgacgaaaag cggatggtcg tgatcctcga
     5041 ccccgaggaa tacctgccct ggctgacctg cggcgagatc gaggccaagc gctacttccg
     5101 gccgtggccc gggatcttgc tcggcgagcc ggcgccgctt gcgcgcccgc ccaaggtcct
     5161 cgccgacccg ccgcccaagg acgagatcaa gccaccgaag gcgcccaagc cgacaccgcc
     5221 gccttcgcct ccgccagcgc agggcgactt gttctagcca cgccagcgcg ctcccggaga
     5281 gcggcgaacg cgggcgcccc gcgacggccc tcgccgccgt cgtcagccgc tcagcgccca
     5341 ccggcccagc acctggtgct cgccctcgcc atggtggctg tcgatcaata ccaggtcggc
     5401 ggcgcgccag cgcacgggtt cgatcgccgc ctcggggatg cgcctcccgc gccaggacat
     5461 cgtcatgtgc ggcgcgatgc gctggcgcac gacgcgctcg cccgtgttcg cgagcgcgat
     5521 gcccagcgcc tcgtgcagcg cgagcaaggc cgcatcgtcg ctgctcgtga agacccacgc
     5581 attgtctttg ccgccgaacg tgcagacgcg gtcgaacacg atgtcgaacg gcgcctgtcg
     5641 caccgccgcc gcggcacggc accaccggtc gacctcggat tccggcggct cgacggcgtg
     5701 cgcgccgagc gcgtgcagcg tcacgtgcag ccggtgcgcg tccgtcgctt tgcccgcgaa
     5761 gcccaacccc tcttcccccc gccgggcatg cgcatgcaac cggatggcat ccgcgccgga
     5821 aggcacgagc gcatagaaca cggagtgcac ccggccaggc acgatggcga gccgcggcgg
     5881 acgcacgaac ggccccatgc cgagcccggc gggcggttca tcgaacagcg aacattggcg
     5941 cagcatcgcg atcctcgagg c
//
LOCUS       DATCRO010000772        14819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_301400, whole genome shotgun sequence.
ACCESSION   DATCRO010000772 DATCRO010000000
VERSION     DATCRO010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14819
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301400"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VIN75_20985"
     CDS             complement(<1..635)
                     /locus_tag="VIN75_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976703.1"
                     /translation="MTTQPDTTPRAIRIDGPLSARAVLDHFNEKGRALLGAGQDDAVG
                     RIAQLLGHEADLSAAWGGLPGTDHPRHWQPEVGDEAALSDPRSDHRRLDVHLPLWWAL
                     PLAVRDRQPQRVVLPLAATREGLAALVRRHMPGGWWNHTVHVALDVETRVYWGARGAV
                     HPDAAARHACAQRGDAGHPSLRLMTLEGGRAVEAAMPDRPADPDVVLKVHPG"
     gene            complement(645..2549)
                     /locus_tag="VIN75_20990"
     CDS             complement(645..2549)
                     /locus_tag="VIN75_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976704.1"
                     /translation="MKREDAPPSSILTLSPDDRPSQFGSVEPLPRPDQHADGDLVPGQ
                     VDPLIDQVDNLHAFARTGLVGNTVGACVVVALYAHVVPALTMVVWAVLFGAALLLRAV
                     LTVTYLRRWRGSPALLRSWRNRYLTGVLTTGTMWGISVWLFYPYGGNLERIALLLTVY
                     SFCISAVPALATQFGAFVAFVALTFVPMILRIALLPEHDGWTLAAILFLIFGITVMLG
                     RSYQRTFSRVVALKQRGMQLLAQLQVEKVAADQARREAETANRAKTQFFAAASHDLRQ
                     PLHALGLFAEALRHRIGTDAEVAHLVNSINSSVDALEGLFSELLDITKIDTGGVEARP
                     ENFSVEAMFARLKLHYEATAFEKGLALRFHGSKHYAYADPTLLDRVVRNLLSNAIRYT
                     NDGGVVVAARQRGDKLRFEVWDTGLGIRPHEQERIFEEFYQVSTDAPPLDPTQRKGLG
                     LGLAIVRRLARVLDAPLRLRSVPGHGTMFSIEVPLGQRPKPTEPVAPRSTSLGLTLDR
                     RLIVIVEDDPAVLGGLQVLLKSWGADVIAFDSFGAAAEWADAAAARKLMKPDLLITDF
                     RLESGHTGIEVIETMRGAFGKGLPSVIVTGSLMSNQEGEAQAHDFHLLLKPVVPTKLR
                     AMIAFKLGQR"
     gene            complement(2546..4123)
                     /locus_tag="VIN75_20995"
     CDS             complement(2546..4123)
                     /locus_tag="VIN75_20995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8976705.1"
                     /translation="MRVAIISVFVDYHRRGAHHRGVLQPQIGPLIAALLPDSADIDIV
                     NDTWVDPDWSKSYDLVFLSCMHADFDRARQISHWFRKRGAQVVLGGSLASMLPQLCAP
                     WFDAVVVGDPEDTVPHVYEDASRRQLQRLYRSGGDAAARAPTPLVHRVAHQQVFPLAL
                     EATRGCPYTCDFCVLTGMGTRFDTRPAARVEHDLVTLREALRGRVTGPLKNKRRRMAM
                     FYDNNIAGNLGWLRQLCDVTERLGLEWASSVTFNVLTNKPLLQRMFDSGCRALYVGLE
                     TFNPAALDDFNKPQNRLPLIRRALDDARDVGILVTAGMILSPEHDDVDYIRSLPRRLR
                     ESGLHVPTFLAFETPFPGTPYFNRLAARNAQRVAANERALFLPRALLRDFTSYTLVVE
                     PTHASAEDYVDAYKDAVDEIYAPRRRLAKLAHDLPSFIRRGAWTSVALDIGDMLTGGL
                     PRAPGRTYLAGSDLAPPERVPLTEADFVDEAEAARLMRPTAVTDEAGRLLPQWAPPDG
                     SLRPVLPPRKNKSAVVP"
     gene            complement(4161..4862)
                     /locus_tag="VIN75_21000"
     CDS             complement(4161..4862)
                     /locus_tag="VIN75_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976706.1"
                     /translation="MKVLLVDDHPLILSALQAMIQELDPDVKVTAVGSAGAARSALAA
                     DADFDLVLLDLQLGDASGFDVLVEMRKAHPALPVVVISASDRASDVIQAIDLGAMGFV
                     PKRATNDTLVDALRLVMAGGIYVPAMHLGASQGPASRSAAPAIPAAETATYQVTPSFE
                     ELGLTPRQADVLTQLLQGKPNKEIARRLGLSVETVKDHVQAVLRALGVSSRTQAVLAV
                     SQMTQQRLERRAVPR"
     gene            complement(5032..5484)
                     /locus_tag="VIN75_21005"
     CDS             complement(5032..5484)
                     /locus_tag="VIN75_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8976707.1"
                     /translation="MTVYEHLADLAALRGQVIGTSGWVEVDQARIDQFAQATGDHQWI
                     HVDRARAAAGPFGTTVAHGFLTLSLLSELATGAFRIADTRMGLNYGLDRVRFPSPVPV
                     GSRLRAVCVLDRHEPIDGGAQLTLTVTIEREGSAKPACVAAWVTRHFV"
     gene            complement(5481..6122)
                     /locus_tag="VIN75_21010"
     CDS             complement(5481..6122)
                     /locus_tag="VIN75_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8976708.1"
                     /translation="MSVIVVANPKGGVGKSTLATQISGFLASQGRLVTLGDLDRQQSS
                     RQWLDIRPPHLRRIEPWEISRDAIARPAKGATDAVIDTPAGLHGNRLDTALKVADIVV
                     VPLQASLFDMQATHAFLQELRAHRRAGRIRMGLVGMRIKEHTIALERFQEFVATLGIP
                     LVTTLRDTQNYVHLAAHGLTLFDVAPGKVERDLAQFEPLARWLLQPAKAAPLE"
     gene            6196..6801
                     /locus_tag="VIN75_21015"
     CDS             6196..6801
                     /locus_tag="VIN75_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8976709.1"
                     /translation="MADVLVLLAHPDLARSRVNRALAAQARLLPSETVQVRDIYALYP
                     DYVIDVEAEQAALAAARTVVWVHPIQWYSMPAMMKLWVDEVLAFRWAYGPGGRALVGK
                     CLWLAASTGGVETSYQKLGGADHPFERFLAPYRQTAEMVGMRFLDPLILHGAHRADAH
                     AVHDHAHAFAQRLLALAGETRAGAVAPPPEVAADERRITSD"
     gene            6801..8705
                     /gene="kefC"
                     /locus_tag="VIN75_21020"
     CDS             6801..8705
                     /gene="kefC"
                     /locus_tag="VIN75_21020"
                     /inference="COORDINATES: protein motif:HMM:NF002924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEY8976710.1"
                     /translation="MSHTHWLTLALTYLAAAVIAVPVARRLGVGAIIGYLGAGFAIGP
                     WGLRLVTDPSQIVDLAEFGVVLMLFLIGLELEPRRLWAMRRPIFGWGSVQLLGSLALI
                     AGIGWACMGFWPDGWRLALVAGAALAMSSTAIGLATLNERNILLTTGGQNILSVSLFQ
                     DIAAIPLLALIPLMAPDRVAGADAGHPALAAAKAFGMIAVIVLGGRLLLRPALRFIAR
                     SQTPEIFTAAALGLVVGTAALMQAVGLSMALGAFLAGVLLAESEYRRELETDIEPFKG
                     LLLGLFFIAVGMGIDLGVVLHNPWQVAAIVVGFLAVKTLVLWGMEVGIPIPRLERPVF
                     TLLLAQGGEFGFVVLQAAQGQQVIDGAQAGILTASITLSMLLTPLLILVADRWWMPLL
                     ARFGPRAKVSELAEPQDAPVIIAGFGRYGQIIGRLLHANGIQATVLDHDAEAIEALRK
                     FGFGVHYGDATRLDLLRIAGAAKAHVLVVAVDGIEPSLELVDIARENFPNLAIVARAR
                     NVQHWYQLNERGVKFIERETLDAALASARSVLELMGYEPHRARTLAMRFRHHSVQQLR
                     DMAPHFRDESKLVSLARAGRQQLEQLFAQERLDHPPVARTWDATQGDESFDELDAERV
                     APAREGPPEA"
     gene            8821..9486
                     /locus_tag="VIN75_21025"
     CDS             8821..9486
                     /locus_tag="VIN75_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8976711.1"
                     /translation="MRILLVEDDPLLSDSTARALRSQGWVVDCTERGEPVAVSVRQDP
                     YDLLILDVGLVGIDGFETLRRVRAQGSDLPVLMLTARDAIEDRVRGLESGADDYLVKP
                     FALSELVARSRALVRRNQARGGSVLSLGNLRMDLEARRAYIGETELALAGREWSILQF
                     MLSRTGKIVAKEQIIAAISNWDGTTSDNAIEVHVSRLRSKLEPAGLKIRTIRGFGYLL
                     EEA"
     gene            9572..11044
                     /locus_tag="VIN75_21030"
     CDS             9572..11044
                     /locus_tag="VIN75_21030"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976712.1"
                     /translation="MTAPAHPDRGLRGLLKIDARPSIRRSLVLWLVLPLALLVPATAI
                     LFYKLALAPALDSLDHSLDGTALAIERLVKARPDGSVDMAMSPELDYALRADRFDVVH
                     WVLQAPDNRVIAGDPELATLGGPPAPVDWRFSDANWEDDPVRVAMHSWICVPATADRA
                     AGRCEARVSETLNKRRSVAKHVSAAAASAMLIEALVLAMMGWLAIARSLRPMVRLSTD
                     IEQRSPEHLGAVDQPDIPREVAPLVTALNGLFARVVAASAAEKAFIADAAHQLRTPLT
                     ALRTETELALLESHPPQTEALLRRLHLATTRSARLANQLLSQARAEHVRHNSAMERFD
                     LKQIATEAAEEWVARSVDAGVDLGFELESARVDGHGFLLREMLANLLDNAINYAGAGA
                     RVTIRTGPSHEAAGCAQLEVEDNGPGIPPADRERAFERFQRGNSGGPGSGLGLSIVRD
                     IASHHGARAELLDGEGGVGLRVRVTFAPLDAAPRKPGVRS"
     gene            11183..11617
                     /locus_tag="VIN75_21035"
     CDS             11183..11617
                     /locus_tag="VIN75_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY8976713.1"
                     /translation="MFQPSQHDVRRFFCETHALARSGAPMSPIQILASQWIAEHPEYH
                     ADLADVDAALAAVFEVDDGRTNPFLHLAMHLTISEQCSIDQPSGIRQAVELLAARRQS
                     LHDAHHAAMDCLGEMIWQSQRSGLPPDGHQYLDCLRRKATRD"
     gene            12132..13664
                     /locus_tag="VIN75_21040"
     CDS             12132..13664
                     /locus_tag="VIN75_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8976714.1"
                     /translation="MINNIVRRPPWRTTASSIAVGLALSACAVGPDFHAPDAPQVVDA
                     SHPYTPAPLPAMAASSPSPAYVPQRFVDGQDISASWWEAFQSPALNALVQSALQHSPT
                     VAAAQATLRQAEENYRADYGSKVFPSVTGQLSAARQRQALAQSPQVPAGTTSNLFQAG
                     LDLSYTLDIAGGNRRELEGVRAAIDVQRYQVESTWLSLTSSVVATAIQEASLRAQVKA
                     TQEVIDNETASFKVISRQVELGAVGRSAALQQQTLIAQSQAQLPALEKALAQTRHQLA
                     VLAGRLPGDAGLPTFDIDTLVLPRELPLSVPSALVRQRPDVRAAEAQLHEASAQIGVA
                     TAALYPQITLSAGVDRESLKLDKLFDTGTTGWSLAAGLVQPIFNGGALRAKKRASEAA
                     YEAAQAQYQSTVLNAFLDVANALRAIDTDAQAVQANADAERLSNEALGLVQRQYQLGA
                     VSYLASLDAQRTALSTRVALVQARAARFTDTAALFRALGGGWWNAPDAVASAAGASSA
                     AR"
     gene            13764..>14819
                     /locus_tag="VIN75_21045"
     CDS             13764..>14819
                     /locus_tag="VIN75_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976715.1"
                     /translation="MLIMIGLVVLLVAALAFGFYLHIQKLIASSPKPAPATVTATKVG
                     SQDWQPTLAAVGSVVAVRGVDVTTEISGMVREILFKSGQDVKKGDVLVELNADADKAQ
                     LGALEAAADLAASVLKRDKAQFEAQAIAQAQVDADAADLKSKRALADQQRANIDKKTI
                     RAPFSGRLGITTVNPGQYVNPGDKIVTLQTIDPVYVDFNLPQKSVGQLAVGQVVNVTT
                     DGFPGTSFPGKISAISPKVDTTTRNVVVEATLPNPKRQLLPGMFANAAVEVGDKKHYL
                     TLPQTAVTYNPYGSTVFVILTADEAAAQARANAASGVGSAAAPAAAASGSGLVVQQAF
                     VTTGETRGDQVAILKGLK"
BASE COUNT         2124 a         5325 c         5261 g         2109 t
ORIGIN      
        1 cccggatgca ccttgagcac gacgtcggga tcggccgggc gatcgggcat cgcggcctcg
       61 accgcgcggc cgccctccag cgtcatcagc ctgagcgacg ggtgccccgc atccccgcgc
      121 tgcgcgcagg cgtgccgcgc ggccgcgtcc gggtgcacgg ccccgcgcgc gccccagtac
      181 acgcgggtct cgacgtccag cgccacgtgc accgtgtggt tccaccagcc gccgggcatg
      241 tggcggcgga ccagcgcggc cagcccctcg cgcgtggcgg ccagcggcag cacgacgcgc
      301 tgcggctggc gatcgcgcac cgccagcggc agcgcccacc acagcggcag gtggacgtcg
      361 agcctgcggt ggtcggagcg ggggtcgctg agcgccgcct cgtcgccgac ttccggctgc
      421 cagtggcgcg gatggtcggt gccgggcagg ccgccccacg cggcgctgag gtcggcctca
      481 tgcccgagca gctgcgcgat gcgccccacc gcgtcgtcct ggccggcgcc cagcagcgcg
      541 cggcccttct cgttgaagtg atcgagcacc gcgcgcgccg acaagggccc gtcgatgcgg
      601 atggcgcgcg gcgtggtgtc gggttgcgtg gtcatgatgc agggtcagcg ttggccgagc
      661 ttgaacgcga tcatcgcgcg cagcttggtg gggacgacgg gcttgagcag caggtggaag
      721 tcgtgcgcct gcgcctcgcc ttcctggttg gacatgaggc tgcccgtgac gatcaccgac
      781 ggcaatccct tgccgaaggc cccgcgcatc gtctcgatca cctcgatgcc cgtgtgcccg
      841 ctctcgaggc ggaagtcggt gatcagcagg tcgggcttca tcagcttgcg cgccgcggcg
      901 gcatcggccc actcggcggc ggcgccgaag gaatcgaagg cgatcacgtc ggcgccccag
      961 ctcttgagca gcacctgcag gccgcccagc acggccgggt cgtcctcgac gatcacgatg
     1021 agccggcgat ccagcgtcag gcccagcgac gtgctgcgcg gagccaccgg ctcggtgggc
     1081 ttgggacgct gtcccagcgg cacctcgatc gagaacatcg tgccgtggcc cggcaccgag
     1141 cgcaggcgca gcggcgcgtc gagcacgcgc gccagccggc gcacgatggc caggcccagg
     1201 cccaggccct tgcgctgcgt cggatccagc ggcggggcgt cggtggagac ctggtagaac
     1261 tcctcgaaga tgcgttcctg ctcgtgcggc cggatgccca ggccggtatc ccagacctcg
     1321 aagcgcagct tgtccccgcg ctggcgcgcc gccaccacga cgccgccgtc gttggtgtag
     1381 cggatcgcgt tggacagcag gttgcgcacc acgcgatcga gcagcgtcgg gtcggcgtag
     1441 gcgtagtgct tgctgccgtg gaaacgcagc gccaggccct tctcgaaggc ggtcgcctcg
     1501 tagtgcagct tcaggcgcgc gaacatcgcc tcgacgctga agttctccgg ccgcgcctcg
     1561 acgccgccgg tgtcgatctt ggtgatgtcc agcagctcgg agaacaggcc ctcgagcgcg
     1621 tcgaccgagc tgttgatgct gttgaccagg tgcgccacct cggcgtcggt gccgatgcgg
     1681 tggcgcagcg cctcggcgaa caggcccagc gcatgcagcg gctggcgcag gtcgtggctg
     1741 gcggcggcga agaactgggt cttggcgcgg ttggccgtct cggcctcgcg gcgcgcctgg
     1801 tcggcggcca ccttctcgac ctgcagttgc gcgagcagct gcatgccgcg ctgcttgagt
     1861 gccacgacgc gcgagaacgt gcgctggtag ctgcgcccca gcatcaccgt gatgccgaag
     1921 atcaggaaca ggatcgccgc cagcgtccag ccgtcgtgct cgggcagcag cgcgatgcgc
     1981 aggatcatgg gcacgaaggt cagcgcgacg aaggcgacga aggcgccgaa ctgcgtggcc
     2041 agcgccggca ccgcggagat gcagaagctg tagacggtca gcagcagcgc gatgcgctcg
     2101 aggttgccac cgtacggata gaagagccac accgagatgc cccacatcgt cccggtggtc
     2161 agcacgccgg tcaggtagcg gttgcgccag ctgcgcagca gcgccggcga gccgcgccag
     2221 cgccgcaggt aggtgaccgt gagcaccgcg cgcagcagca gcgcggcgcc gaacagcacg
     2281 gcccacacga ccatcgtcag cgccggcacg acgtgcgcgt acagcgccac gaccacgcag
     2341 gcgccgacgg tgttgcccac caggccggtg cgcgcgaacg cgtgcaggtt gtcgacctgg
     2401 tcgatcagcg ggtcgacctg gcccggcacg aggtcgccgt cggcatgttg gtccggccgg
     2461 ggcagcggct cgacgctgcc gaactgcgag ggccggtcgt cgggcgacag cgtcaggatg
     2521 gacgacgggg gagcatcttc gcgcttcatg ggacgacggc cgatttgttc ttgcggggag
     2581 gaaggacggg ccgcagcgag ccgtccgggg gcgcccattg tggcaacagg cggcccgcct
     2641 cgtccgtgac ggcggtgggc cgcatcagcc gcgccgcctc ggcctcgtcg acgaagtcgg
     2701 cctcggtcag cggcacgcgc tcgggcggcg cgaggtcgga gcccgcgagg taggtgcggc
     2761 ccggcgcgcg cggcaggccg ccggtgagca tgtcgccgat atccagtgcc acgctcgtcc
     2821 acgcgccgcg gcggatgaac gacggcaggt cgtgcgcgag cttggccagg cgccggcgcg
     2881 gcgcgtagat ctcgtcgacg gcgtccttgt acgcgtcgac gtagtcctcg gcgctggcat
     2941 gcgtgggctc gacgaccagc gtgtacgacg tgaagtcgcg cagcagcgcg cgcggcagga
     3001 acagcgcgcg ctcgttggcg gccacccgct gcgcgttgcg cgccgcgagc cggttgaagt
     3061 acggcgtgcc ggggaacggc gtctcgaacg cgaggaacgt gggcacgtgc agcccgctct
     3121 cgcgcaggcg gcgcggcagc gagcggatgt agtcgacgtc gtcgtgctcg gggctcagga
     3181 tcatgcccgc cgtcacgagg atgcccacgt cgcgcgcgtc gtcgagcgcc cggcggatca
     3241 acggcaggcg gttctgcggc ttgttgaagt cgtcgagcgc ggccgggttg aacgtctcca
     3301 ggccgacgta cagcgcgcgg cagccgctgt cgaacatgcg ctgcagcagc ggcttgttgg
     3361 tgagcacgtt gaacgtgacg gagctggccc actccaggcc caggcgctcg gtgacgtcgc
     3421 acaactggcg cagccagccg aggttgccgg cgatgttgtt gtcgtagaac atcgccatgc
     3481 gccggcgctt gttcttgagc gggccggtga cgcgtccccg cagcgcctcg cgcagcgtga
     3541 cgaggtcgtg ctcgacgcgc gcggccgggc gcgtgtcgaa gcgcgtgccc atgccggtga
     3601 gcacgcagaa gtcgcaggtg tacgggcagc cgcgcgtggc ctcgagcgcg agcgggaaga
     3661 cctgctggtg ggccacgcgg tgcaccagcg gcgtgggcgc ccgcgccgcg gcgtcgccgc
     3721 cggagcggta caagcgctgc agctgccggc gcgaggcgtc ctcgtacacg tgcggcaccg
     3781 tgtcctcggg gtcgcccacg accacggcat cgaaccaggg cgcgcacagc tgcggcagca
     3841 tcgacgccag cgacccgccc agcaccacct gggcgccgcg cttgcggaac cagtgcgaga
     3901 tctgccgcgc gcggtcgaag tccgcgtgca tgcacgacag gaacaccagg tcgtaggact
     3961 tggaccagtc ggggtcgacc caggtgtcgt tgacgatgtc gatgtccgcc gagtcgggca
     4021 gcagcgccgc gatcagcggg ccgatctgcg gctgcagcac gccgcggtgg tgggcgccgc
     4081 ggcggtggta gtcgacgaag acggagatga tcgcgacgcg catgcttgcg tgccgatcga
     4141 tgcgtctgtt caggatctct ctagcggggg acagcgcggc gttccagccg ctgctgcgtc
     4201 atctggctga ccgccagcac ggcctgcgtg cgcgaactca cgcccagcgc ccgcagcacg
     4261 gcctggacgt gatccttgac cgtctcgacc gacagtccca ggcggcgcgc gatctccttg
     4321 ttcggcttgc cctgcagcag ttgggtgagc acgtcggcct ggcgcggggt caggcccagc
     4381 tcctcgaacg acggcgtgac ctggtaggtg gccgtctcgg ccgccgggat ggcaggagcg
     4441 gccgagcggc tggccgggcc ctggctggcg cccaggtgca tggccggcac gtagatgccg
     4501 ccggccatca ccaggcgcag cgcgtcgacc agcgtgtcgt tggtggcccg cttgggcacg
     4561 aagcccatcg cgcccaggtc gatggcctgg atgacgtcgc tggcgcggtc ggaggccgag
     4621 atcaccacca ccggcagcgc cgggtgggcc ttgcgcatct cgacgaggac gtcgaagccg
     4681 ctggcgtcgc ccagttgcag gtcgagcagc acgaggtcga agtcggcgtc cgccgcgagc
     4741 gcggaacgcg ccgcccccgc gctgccgacg gccgtgacct tcacgtccgg atccagctcc
     4801 tggatcatgg cctgcagcgc cgacaggatc aacgggtggt catcgaccag caacaccttc
     4861 atggaaactc cgctcggact ctcgttatgg gtcgtccgcc gcatcggccg cgggcgacat
     4921 ttccctcgtc ggaatcgtac cggctgaccc gtgctttgcc gcccccccga tggggggggc
     4981 agggaaaacg actgtcgagc agaaacgacg cgccggcgcc ttcgtgtccg ttcagacaaa
     5041 gtggcgggtg acccaggcgg ccacgcaagc cggcttggcg ctgccctcgc gctcgatggt
     5101 gacggtgagc gtcaattgcg cgccgccgtc gatcggttca tggcgatcga gcacgcacac
     5161 cgcgcgcagg cggctgccca cgggcaccgg cgatgggaag cgcacgcggt cgaggccgta
     5221 gttcaggccc atgcgggtgt cggcgatgcg gaaggcgccg gtcgccagct ccgacagcag
     5281 cgacagcgtc aggaagccgt gggccacggt ggtgccgaac ggcccggccg cggcgcgcgc
     5341 ccggtcgacg tggatccact ggtggtcgcc ggtggcctgg gcgaactggt cgatgcgcgc
     5401 ctggtcgacc tcgacccagc cgctggtgcc gatcacctgg ccccgcagcg cggccaggtc
     5461 ggccaggtgc tcgtagacgg tcattcgagc ggggcggcct tggccggctg cagcagccag
     5521 cgcgccagcg gctcgaactg cgccaggtcg cgctccacct tgccgggcgc gacgtcgaac
     5581 agcgtcaggc cgtgggcggc caggtgcacg tagttctgcg tgtcgcgcag cgtcgtgacc
     5641 agcgggattc ccagcgtggc gacgaattcc tggaagcgct cgagcgcgat cgtgtgctcc
     5701 ttgatgcgca tgcccaccag gcccatgcgg atgcggccgg cgcgccggtg ggcgcgcagt
     5761 tcctgcagga aggcgtgcgt ggcctgcatg tcgaacaggc tggcctgcag cgggacgacg
     5821 acgatgtcgg ccaccttcag cgcggtgtcc aggcggttgc cgtgcaggcc ggcgggggtg
     5881 tcgatgacgg cgtcggtggc gcccttggcc ggccgggcga tggcgtcgcg cgagatttcc
     5941 cacggctcga tccgtcgaag gtggggcggg cggatgtcca gccactggcg cgacgactgc
     6001 tggcggtcga gatcccccag ggtgaccagc ctgccctgcg aggcgaggaa tcccgagatc
     6061 tgcgtcgcga gcgtgctctt gccgacgccg cccttgggat ttgctaccac gatgaccgac
     6121 atgcgcgtgc tctccggtgt gtcgttccag ctcgacggga atggcgatgt taccgaaccc
     6181 cgccacaatg ggtccatggc agacgttctg gtgctgttgg cccaccccga cctcgcgcgt
     6241 tcgcgcgtca accgcgcgct cgccgcccag gcgcggctgc tgccgtccga gacggtgcag
     6301 gtgcgcgaca tctacgcgct ctatccggac tacgtcatcg acgtcgaggc cgaacaggcc
     6361 gcgctcgccg cggcgcgcac ggtggtgtgg gtgcatccga tccagtggta ctcgatgccc
     6421 gccatgatga agctgtgggt cgacgaggtg ctggccttcc gctgggccta cgggccgggc
     6481 ggccgggcgc tggtgggcaa gtgcctgtgg ctggcggcgt ccacgggcgg cgtcgagacc
     6541 tcgtaccaga agctgggcgg cgccgaccac ccgttcgagc gcttcctggc gccctaccgc
     6601 cagacggccg agatggtggg catgcgcttc ctcgacccgc tgatcctgca cggcgcccat
     6661 cgcgccgacg cgcacgccgt gcacgaccac gcgcatgcgt tcgcgcaacg cctgctcgcg
     6721 ctcgcgggcg agacgcgcgc cggcgcggtc gcgccgccgc ccgaggtcgc cgccgacgaa
     6781 cgccgcatca cgagcgattg atgtctcata cgcactggct caccctcgcc ctcacctacc
     6841 tggccgcggc cgtcatcgcc gtgccggtcg cccgccgcct gggcgtgggc gccatcatcg
     6901 gctacctcgg cgccggcttc gccatcggcc cgtggggcct gcggctggtg acggatccgt
     6961 cgcagatcgt cgacctggcc gagttcgggg tcgtgctgat gctgttcctc atcggcctgg
     7021 agctcgagcc caggcggctg tgggcgatgc gccggcccat cttcggctgg ggcagcgtgc
     7081 agctcctggg cagcctcgcg ctgatcgccg gcatcggctg ggcctgcatg ggcttctggc
     7141 cggacggctg gcggctcgcc ttggtggccg gcgcggccct ggcgatgtcg tcgacggcca
     7201 tcggcctggc cacgctcaac gaacgcaaca tcctgctgac gaccggcggc cagaacatcc
     7261 tgagcgtgtc gctgttccag gacatcgccg ccatcccgct gctggcgctg atcccgctga
     7321 tggcgcccga ccgcgtggcc ggggccgacg cgggccatcc ggcgctggcc gcggccaagg
     7381 cgttcggcat gatcgccgtc atcgtcctcg gcggccgcct cctgctgcgg ccggcgctgc
     7441 gcttcatcgc gcgcagccag acgcccgaga tcttcaccgc cgcggccctg ggcctggtgg
     7501 tgggcaccgc ggcgctgatg caggccgtgg ggctgtcgat ggcgctgggc gccttcctgg
     7561 ccggcgtgct gctggccgag agcgagtacc ggcgcgagct cgagaccgac atcgagccgt
     7621 tcaagggcct gctgctgggg ctgttcttca tcgcggtggg catgggcatc gacctgggcg
     7681 tcgtcctgca caacccgtgg caggtggccg ccatcgtcgt gggctttctc gccgtgaaga
     7741 cgctggtgct gtggggcatg gaggtcggca tcccgatccc gcgcctggag cgcccggtct
     7801 tcacgctgct gctcgcgcag ggcggcgagt tcggcttcgt cgtgctgcag gccgcgcagg
     7861 gccagcaggt gatcgacggc gcgcaggccg gcatcctgac cgcgtccatc acgctgtcga
     7921 tgctgttgac gcctctgctg atcctggtgg ccgaccgttg gtggatgccg ctgctggccc
     7981 ggttcggccc gcgggcgaag gtctccgagc tggccgagcc gcaggacgcg ccggtgatca
     8041 tcgcgggctt cggccgctac ggccagatca tcggccgcct gctgcacgcc aacggcatcc
     8101 aggcgacggt gctcgatcac gacgccgagg ccatcgaggc gctgcgcaag ttcggcttcg
     8161 gcgtgcacta cggcgacgcc acgcggctgg acctgctgcg catcgccggc gccgcgaagg
     8221 cgcacgtgct ggtggtggcc gtcgacggca tcgagcccag cctcgagctg gtggacatcg
     8281 cgcgcgagaa cttccccaat ctcgcgatcg tggcgcgcgc ccgcaacgtg cagcactggt
     8341 accagctcaa cgaacgcggc gtgaagttca tcgagcgcga gacgctggac gccgcgctcg
     8401 ccagcgcccg cagcgtgctc gagctgatgg gctacgagcc gcaccgcgcc cgcacgctcg
     8461 cgatgcgctt ccgccaccac tccgtgcagc agctgcgcga catggcgccg cacttccgcg
     8521 acgagtccaa gctggtctcg ctcgcgcgcg ccgggcgcca gcagctcgaa caactgtttg
     8581 cacaggaacg cctcgaccat ccgccggtgg ccaggacgtg ggacgccacg caaggcgacg
     8641 agtcgttcga cgagctcgac gccgagcgcg tggcgcccgc cagggaaggg ccgccagagg
     8701 cttgaggcac gcggttctgg cgcgcaagct tgggctcagg tatcgttaag cgacccctga
     8761 gcctgccctt cgatgatgcg ccgaacccca cctcccatcg ctcccatcgg agtgccccaa
     8821 gtgcgcatcc ttcttgtcga agacgacccg cttctttccg actccaccgc gcgcgccctg
     8881 cgctcccagg gctgggtcgt cgactgcacc gaacgcggcg agcccgtcgc cgtttccgtc
     8941 cgccaggatc cgtacgacct gctcatactc gatgtcggcc tcgtcggcat cgacgggttc
     9001 gagacgctgc gccgcgtgcg cgcgcagggc tcggacctgc cggtgctgat gctgaccgcg
     9061 cgcgacgcca tcgaggaccg cgtgcgcggc ctggaaagcg gcgccgacga ctacctcgtc
     9121 aagccgttcg cactgagcga gctcgtcgcg cgttcgcgcg cgctggtgcg ccgcaaccag
     9181 gcgcgcggcg gcagcgtgct cagcctgggc aacctgcgca tggacctcga ggcgcgccgc
     9241 gcctacatcg gcgagacgga gctggcgctg gccggccgcg aatggtcgat cctgcagttc
     9301 atgctgagcc gcaccggcaa gatcgtggcc aaggaacaga tcatcgcggc gatctccaac
     9361 tgggacggca ccacctccga caacgcgatc gaggtgcacg tgtcgcgcct gcgctcgaag
     9421 ctggagccgg ccggcctgaa gatccgcacc atccggggct tcggctacct gctcgaagag
     9481 gcctgagcgc cgcgcggcgc gccggttccg ggacccctgc tcgactgggc gatactggcg
     9541 catgaacccg cgtgccgaac gcgacatgcc cgtgaccgct cccgcacacc ccgaccgcgg
     9601 cctgcgcggc ctgctgaaga tcgatgcccg ccccagcatc cgccgatcgc tggtgctgtg
     9661 gctcgtcctg ccccttgcgc tgctggtgcc cgccacggcc atcctgttct acaagctcgc
     9721 gctggcgccg gcgctcgact cgctcgacca ctcgctcgac ggcacggcgc tcgccatcga
     9781 gcgcctcgtc aaggcccgcc ccgacggcag cgtcgacatg gccatgtcgc ccgagctcga
     9841 ctacgcgctg cgcgccgacc gcttcgacgt cgtgcactgg gtgctgcagg cgccggacaa
     9901 ccgcgtcatc gccggcgatc ccgaactggc cacgctcggc gggccgcccg ccccggtgga
     9961 ctggcgtttc tccgacgcca actgggaaga cgaccccgtc cgcgtggcga tgcacagctg
    10021 gatctgcgtg cccgccaccg cggatcgcgc cgccggccgc tgcgaggcac gggtgtccga
    10081 gacgctcaac aagcggcgca gcgtggccaa gcacgtgtcg gccgcggccg cctcggcgat
    10141 gctgatcgag gccctggtgc tcgcgatgat gggctggctc gcgatcgcgc gcagcctgcg
    10201 gccgatggtg cggctcagca cggacatcga gcagcgctcg cccgagcacc tgggcgcggt
    10261 cgaccaaccc gacatcccgc gcgaggtcgc cccgctggtg acggcgctca acggcctgtt
    10321 cgcgcgcgtg gtcgcggcgt cggccgccga gaaggccttc atcgcggatg cggcccacca
    10381 gctgcgcacg cctctgaccg ccctgcgcac ggagaccgaa ctcgcgctgc tggaatcgca
    10441 cccgccgcag accgaggcgc tgctgcgccg cctgcacctg gccaccacgc ggtcggcgcg
    10501 cctggccaac cagttgctgt cgcaggcgcg cgccgagcac gtgcgccaca actcggcgat
    10561 ggagcgtttc gacctgaagc agatcgccac cgaggccgcc gaagagtggg tcgcacgttc
    10621 cgtggacgct ggcgtcgacc tcggcttcga gctggagagc gcgcgcgtcg acggccacgg
    10681 cttcctgctg cgcgagatgc tcgccaacct gctggacaac gccatcaact acgccggcgc
    10741 cggcgcacgc gtcacgatcc gcaccggccc gtcgcacgag gccgcgggct gcgcccagct
    10801 cgaggtggag gacaacggcc cgggcatccc gccggccgat cgcgagcgcg cgttcgagcg
    10861 cttccagcgc ggcaattccg gcggccccgg cagcggcctg ggcctgtcca tcgtgcgcga
    10921 catcgccagc caccacggcg cccgtgccga gctgctggac ggcgagggcg gcgtcggcct
    10981 gcgcgtgcgc gtcacgttcg ccccgctcga cgccgcgccg cgcaaacccg gggtcaggtc
    11041 ttgaatcttg cggggtcagg tcttgaatgt tgataccccc aggggtagca acattcaaga
    11101 cctgaccccg gtttgttcaa gacctgaccc tgccctgctg cttcagcggc gtcccaagcg
    11161 catccgcgcg acaatcggcc ccatgttcca gccgtcccag cacgacgtcc gccgcttctt
    11221 ctgcgagacc catgcgctcg ccaggagcgg cgcgccgatg tcgccgatcc agatcctcgc
    11281 ctcgcaatgg atcgccgagc accccgagta ccacgccgac ctcgccgacg tggacgccgc
    11341 gctggcggcc gtgttcgagg tggacgacgg ccgcaccaat ccgttcctgc acctggcgat
    11401 gcacctgacg atcagcgagc agtgcagcat cgaccagccc agcggcatcc gccaggccgt
    11461 cgagttgctc gccgcgcggc ggcagtcgct gcacgacgcg caccatgcgg cgatggattg
    11521 cctgggcgag atgatctggc agtcgcagcg cagcggcctg ccgccggacg gccaccagta
    11581 cctggactgc ctgcggcgca aggccacgcg cgactgaagc gcgccgcggg cctggcccgt
    11641 gccgtcagtc gcgtggagag gccgtcatcc cccgttcagc gatggggcgc ctcgcccctc
    11701 cggcctccgt cgacctgaac atcgcctgac aacgcgggcc catcgaaagg ggccagtaag
    11761 ccgggccggc gcatgctgcc gcgctggctg gctgcgccat tcacgggccg ctccggtcgc
    11821 ctcgagtctc tttttcgcgc gggattgtcg attccggccc atcgcgttcg tcgaagcagg
    11881 agttcgaggt gccggacccg tcgttcgtcg cggagccggc ggctggcgct gtaaggcggt
    11941 gttaacgtca agcgccggcc ccgatcgagc gtcaagcctt tttgtccaca aaatcaaaag
    12001 acgtgccggc attatttgct taggcagtag ttgcgtaggg gaatagctgg caggcgggaa
    12061 aacccgacta ccatgccccg ctgaccgtcg cgcccacagg cagcgacgcg cctgaatacc
    12121 gaggagcagg aatgatcaac aacatcgtcc gacgtccgcc ctggcgcacc accgcctcga
    12181 gcatcgccgt cggcctggcc ctgtcggcgt gcgccgtcgg ccccgacttc cacgcgcccg
    12241 acgcgccgca ggtcgtcgac gcgagccacc cgtacacgcc cgcccccctc ccggcgatgg
    12301 ctgccagcag ccccagcccg gcctacgtgc cgcagcgctt cgtcgacggc caggacatct
    12361 cggccagctg gtgggaggcg ttccagtcgc cggccctcaa tgcgctcgtg cagtcggcgc
    12421 tgcagcacag ccccaccgtc gccgcggccc aggcgacgct gcgccaggcc gaggagaact
    12481 accgcgccga ctacggctcg aaggtcttcc cgtcggtcac cggccagctg agcgcggcgc
    12541 gccagcgcca ggcgctcgcg cagtcgccgc aggtgccggc cggcaccacg tccaacctct
    12601 tccaggccgg gctcgacctg tcgtacaccc tggacatcgc cggcggcaac cggcgcgagc
    12661 tcgaaggcgt gcgcgcggcg atcgacgtcc agcgctacca ggtcgagagc acgtggctga
    12721 gcctgacctc cagcgtcgtg gccacggcga tccaggaagc ctcgctgcgg gcgcaggtca
    12781 aggccacgca ggaggtcatc gacaacgaga cggcctcctt caaggtcatc tcccggcagg
    12841 tcgagctggg cgccgtcggg cgctccgcgg cgctgcagca gcagacgctc atcgcgcagt
    12901 cgcaggcgca gctgcccgcg ctcgagaagg cgctcgcgca gacgcgccac cagctggccg
    12961 tgctggccgg ccgcctgccc ggcgacgccg gcctgccgac cttcgacatc gacacgctcg
    13021 tgctgccgcg cgagctgccg ctgagcgtgc cgtccgcgct ggtgcgccag cgccccgacg
    13081 tgcgcgccgc cgaggcgcag ctgcacgagg cgagcgcgca gatcggcgtc gccaccgcgg
    13141 cgctgtatcc gcagatcacg ctgagcgccg gcgtcgatcg cgagagcctc aagctcgaca
    13201 agctgttcga cacgggcacc accggctggt cgctggccgc gggcctggtg cagccgatct
    13261 tcaacggcgg cgcgctgcgg gcgaagaagc gtgcgtccga agccgcgtac gaggccgcgc
    13321 aggcgcagta ccagtcgacc gtgctcaacg cgttcctcga cgtggccaac gcgctgcgcg
    13381 cgatcgacac cgatgcccag gccgtgcagg ccaacgcgga cgccgagcgc ctgtccaacg
    13441 aggcgctggg cctcgtgcag cgccagtacc agctgggcgc cgtcagctac ctggcctcgc
    13501 tcgacgcgca gcgcacggcg ctgtccacgc gcgtcgcgct ggtgcaggcg cgcgcggccc
    13561 gcttcaccga caccgcggcg ctgttccggg cgctgggcgg cggctggtgg aacgcccccg
    13621 atgccgtggc ctcggccgcc ggcgcatcgt cggccgcacg ctgacatccg acaacgaatc
    13681 ctgcgcccgc cggcaccctt cgaacgccgg cggccgcgaa gaacatcgaa cacgaaatct
    13741 ggagtgagaa catggcgaag cggatgctca tcatgattgg cctggtggtg ctgctggtgg
    13801 cggcccttgc cttcggcttc tacctgcaca tacagaagct gatcgccagc tcgcccaagc
    13861 cggcgccggc gacggtcacg gccaccaagg tcggctcgca ggactggcag cccacgctgg
    13921 cggccgtcgg cagcgtcgtg gcggtgcgcg gcgtggacgt caccaccgag atctcgggca
    13981 tggtgcgcga gatcctcttc aagtccggcc aggacgtgaa gaagggcgac gtgctcgtcg
    14041 agctcaacgc cgacgccgac aaggcgcagc tcggcgccct cgaggccgcc gccgacctgg
    14101 cggccagcgt gctcaagcgc gacaaggcgc agttcgaggc gcaggccatc gcgcaggcgc
    14161 aggtcgacgc cgacgcggcc gatctcaaga gcaagcgcgc gctggccgac cagcagcgcg
    14221 ccaacatcga caagaagacg atccgcgcgc cgttctcggg ccgcctgggc atcaccactg
    14281 tcaaccccgg ccagtacgtc aacccgggcg acaagatcgt gacgctgcag acgatcgacc
    14341 cggtctacgt cgacttcaac ctgccgcaga agtcggtggg ccagctggcg gtcgggcagg
    14401 tcgtcaacgt gaccaccgac ggcttccccg gaacgagctt ccccggcaag atcagcgcga
    14461 tcagcccgaa ggtggacacg acgacgcgca acgtggtcgt cgaggcgacg ctgcccaacc
    14521 cgaagcgcca gctgctgccg ggcatgttcg ccaacgcggc ggtcgaggtg ggcgacaaga
    14581 agcactacct gacgctgccg cagaccgccg tcacctacaa cccgtacggc tcgacggtgt
    14641 tcgtcatcct gacggccgac gaggccgccg cgcaggcgcg cgcgaacgcc gccagcggcg
    14701 tgggctcggc cgcggcgccg gccgcggccg cgtcgggctc gggcctcgtc gtgcagcagg
    14761 cgttcgtcac caccggcgag acccgcggcg accaggtggc catcctcaag ggcctgaag
//
LOCUS       DATCRO010000773         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_267790, whole genome shotgun sequence.
ACCESSION   DATCRO010000773 DATCRO010000000
VERSION     DATCRO010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267790"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VIN75_21050"
     CDS             <1..640
                     /locus_tag="VIN75_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976716.1"
                     /translation="VAAVKAYQQARFERTHVDLFEHARYGRAARFFVDELYGPQDFAQ
                     RDAQFGRIVPALVRLFPDDIVETVEALAEVHALSERLDSTMAGHLHGEAPSRASYVRA
                     WQATGEPSSRLRQLELVMRVGGSLDRHTRSLVLRTSLKAMRGPARAADLGSLQAFLEA
                     GFDAFGAMRGAREFLATIEARETALVLRLFEPAAVAAATGSPASDDPLAQLP"
     gene            690..2411
                     /locus_tag="VIN75_21055"
     CDS             690..2411
                     /locus_tag="VIN75_21055"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY8976717.1"
                     /translation="MNDAIASAALTPPRPWLTQYPPGVPGTVAVDTYASLVPLLEESW
                     TKHAKRHAAACMGVRFSYGDVDERSRALGAWLQAKGVRRGDRVAIMMPNVPQYLVAIG
                     AVLRVGGVVVNVNPLYTARELQHQLKDAGATVIIVLENFAHTLETVLAQTDVRHVVLA
                     AMGDMLGFTKGQLVNFVLRHVKKMVPEFRLPLDDGRSVTRFNMAVAQGTRLSLQPVAL
                     AGDDIAFLQYTGGTTGVSKGATLLHRNVVANILQSEAWFKPMFEQLGDKPLSIVCALP
                     LYHIYALTICYMFGARLGAMNILVPNPRDIPGFIKTLKAYKVNMFPAVNTLFNALAND
                     PEFARLDFSELVVSNGGGMAVQQATAERWLSITGCPVVEGYGLSETSPVATSNRLDKR
                     EFTGTIGLPIPSTDIAIRDDDGVDVPAGTAGEICIRGPQVMAGYWKRPDETAKVMTDD
                     GFFRSGDIGIMDEQGYVRIVDRKKDMILVSGFNVYPNEIEQVVNLHPGVLECAAIGVP
                     DERSGEAVKLFVIRRDPALAEEDIAAYCRENFTAYKRPKHIEFRDELPKSNVGKILRR
                     ELRSPTS"
     gene            2470..>2958
                     /locus_tag="VIN75_21060"
     CDS             2470..>2958
                     /locus_tag="VIN75_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8976718.1"
                     /translation="MRREVTTGPGSATSRLLETLGLRVLERGWLSANQAVFHAAGPTP
                     ATVVDTGFSAHAEQTQALLAHALDGAPLGRIVNTHLHSDHCGGNAALQARGDIETWIP
                     SPSMAAVGRWDEDALSYRLTDQPCPRFVADRALVPGEAITLGETAWQIHAAPGHDMDA
                     VML"
BASE COUNT          467 a         1045 c          994 g          452 t
ORIGIN      
        1 cgtcgcggcc gtcaaggcgt accagcaggc gcgcttcgag cgcacgcacg tcgacctgtt
       61 cgagcacgca cgctacggcc gcgcggcgcg cttcttcgtc gacgagctgt acggcccaca
      121 ggatttcgcg cagcgcgacg cgcagttcgg ccgcatcgtg cccgccctcg tgcggctgtt
      181 cccggacgac atcgtcgaga cggtggaggc gctggccgaa gtgcatgcgc tgtccgaacg
      241 gctcgactcc acgatggccg gacatctcca cggcgaggcg ccttcccgcg cgagctacgt
      301 gcgcgcctgg caggcgacgg gcgagccgtc gagccgcctg cgccagctcg agctggtgat
      361 gcgcgtcggc gggtcgctcg atcgccacac ccgcagtctc gtcctgcgca ccagcctgaa
      421 ggccatgcgc ggcccggcgc gcgccgcgga cctgggatcg ctgcaggcgt tcctcgaagc
      481 cggcttcgat gcgttcggtg cgatgcgggg cgcgcgcgag ttcctcgcca cgatcgaggc
      541 acgcgagacc gcgctggtcc tgcgcctgtt cgagcccgcc gctgtcgcgg ccgcgacagg
      601 ttctccggcg tcggacgacc cgctggccca gttgccatag ccggtttccc ggggtgcggc
      661 ggcgtcccgg tggacgaaaa tccggagtca tgaacgacgc catcgcatcc gccgccctca
      721 ctccgccgcg tccctggctg acgcaatacc cgcccggcgt acccggcacc gtcgcggtcg
      781 acacctacgc atcgctggtg ccgctgctcg aggagtcgtg gaccaagcat gcgaagcgcc
      841 atgccgccgc ctgcatgggc gtgcgcttca gctacggcga cgtcgacgag cgctcgcgcg
      901 cgctgggcgc ctggctgcag gccaagggcg tgcggcgcgg cgaccgcgtg gccatcatga
      961 tgcccaacgt gccgcagtac ctggtggcga tcggcgccgt gctgcgcgtg ggcggcgtcg
     1021 tcgtcaacgt gaacccgctg tacacggcgc gcgagctgca gcaccagctc aaggatgccg
     1081 gggcgaccgt catcatcgtg ctcgagaact tcgcgcacac gctcgagacg gtgctcgcgc
     1141 agaccgacgt gcgacacgtc gtgctggcag cgatgggtga catgctgggc ttcacgaagg
     1201 ggcagctcgt caacttcgtg ctgcgccacg tcaagaagat ggtgcccgaa ttccgcctgc
     1261 cgctggacga cggccgcagc gtcacgcgct tcaacatggc ggtcgcgcaa ggcacgcggc
     1321 tgagcctgca gccggtggcg ctggcgggcg acgacatcgc gttcctgcag tacacgggcg
     1381 gcaccaccgg cgtgtccaag ggcgcgacgc tgctgcatcg caacgtcgtg gccaacatcc
     1441 tgcagagcga ggcctggttc aagccgatgt tcgagcagct gggcgacaag ccgctgtcca
     1501 tcgtgtgcgc tcttccgctt tatcacatct atgcgctgac catctgctac atgttcggcg
     1561 cacgcctggg cgcgatgaac atcctcgtgc cgaacccgcg cgacatcccc ggcttcatca
     1621 agacgctcaa ggcctacaag gtcaacatgt tccctgccgt gaacacgctg ttcaatgcgc
     1681 tggccaacga cccggaattc gccaggctcg acttctccga gctggtggtc tccaacggcg
     1741 gcggcatggc cgtgcagcag gcgacggcgg aacgctggct gtcgatcacg ggctgcccgg
     1801 tggtcgaggg ctacggcctg tcggagactt cgccggtggc caccagcaac cgcctcgaca
     1861 agcgcgagtt cacgggcacg atcggcctgc cgatcccgtc gaccgacatc gcgatccgcg
     1921 acgacgacgg cgtggacgtg cccgccggca ccgcgggcga gatctgcatc cgcggcccgc
     1981 aggtgatggc cggctactgg aagcgtccgg acgagacggc caaggtgatg accgacgacg
     2041 gcttcttccg ctcgggcgac atcggcatca tggacgaaca gggctatgtg cgcatcgtcg
     2101 atcgcaagaa ggacatgatc ctggtgtccg gcttcaacgt gtatccaaat gaaatcgagc
     2161 aggtcgtgaa cctgcatccg ggcgtgctcg aatgcgccgc gatcggcgtg cctgacgaac
     2221 gatcgggcga ggccgtgaag ttgttcgtca ttcggcgcga tcccgcgctg gcagaggagg
     2281 acatcgccgc gtattgccgc gagaatttca cggcgtacaa gcgtccgaag cacatcgaat
     2341 tccgggacga actgccgaag tcgaacgtcg gcaagatcct gcggcgcgaa ctgcgttctc
     2401 cgacatcctg aaggttccct cgcccgcatc gtgcctgtcg cctcgtcgcc cgctccactg
     2461 ccagcctcga tgcgccggga agtcaccacc gggccgggca gcgccacgtc gcggctgctc
     2521 gagaccctcg gcctgcgcgt gctcgaacgc ggttggctgt cggccaacca ggccgtcttc
     2581 cacgccgccg ggccgacgcc cgcgacggtc gtcgacaccg gattcagcgc gcatgccgag
     2641 cagacccagg ccctgctcgc ccacgccctg gacggcgcgc cgctcgggcg catcgtcaac
     2701 acgcacctgc actccgacca ctgcggcggc aacgcggcat tgcaggcgcg cggcgacatc
     2761 gagacctgga tcccgtcgcc cagcatggcc gcggtcggac gctgggacga agacgcgctg
     2821 agctaccgcc tgaccgacca accctgcccg cgcttcgtcg cggatcgcgc gttggtgccc
     2881 ggcgaggcga tcacgctggg cgagaccgca tggcagatcc acgcggcgcc cggccacgac
     2941 atggacgcgg tgatgctg
//
LOCUS       DATCRO010000774         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_594181, whole genome shotgun sequence.
ACCESSION   DATCRO010000774 DATCRO010000000
VERSION     DATCRO010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594181"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..513
                     /locus_tag="VIN75_21065"
     CDS             256..513
                     /locus_tag="VIN75_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976719.1"
                     /translation="MSKARVFGTYRPRLNIAPLLLTPGFAAIGYAVRGSQGAWWAVAA
                     WCAVVLGAAVSCTLHAWRSAQEALPHAGRRAFDYDVNNAGR"
     gene            complement(826..1020)
                     /locus_tag="VIN75_21070"
     CDS             complement(826..1020)
                     /locus_tag="VIN75_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976720.1"
                     /translation="MTSQQGSNSGQQKQQEQSSGSSSRSQQGGGSKQSGNQASDRQGS
                     RGGSSEQHSNAGKQSHKNER"
     gene            complement(1391..>3656)
                     /locus_tag="VIN75_21075"
     CDS             complement(1391..>3656)
                     /locus_tag="VIN75_21075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8976721.1"
                     /translation="ATVFMDRSLRITRYTPSAVELFNLIPTDVGRPLSDLSNHLSYPE
                     LDADARRVLSSLVPLEREVNDARQHWFLVRMLPYRVGEDRIAGIVLTLVDITERKQNQ
                     EALRVSEQRFGAIADKAAVGVLQASEAGDITFSNRYMSRLLGYGEGELIGKPMRELVH
                     PDDRAESDARLARLREQGSCEIEKRLLCRDGRVLWVHNSVSWLQTQPGEPAEVLVVCL
                     DISVRKQAEDALRASEERLRLMMDNATEYAILSLDSNHRVTSWSVGAQRLLGWSEDEI
                     IGQHYDKIFGTDERAGDVPRSEAETALAKGWASDERPLQRKNGERFWASGVMMPMRDH
                     RGDVVGFVKILRDQTADRQSRDELARSRADLVDALSQNEQARDQLQAADLAKDRFLAV
                     LSHELRNPLASIANATDALQAASRLTPVDRGRAQLTLRSQVEAMRALLDDLLDVSRLR
                     FGRLALKKQDVTLRSIVETALDTSRALADRRGHTLTVRLPDEEIMLNADPTRMGQVLS
                     NLLINAAKYTDEGGHIELRASVEGGQCRIEVADDGKGLDEAARRSMFEMFWRASEFDV
                     GSGQGMGIGLSVARSVVQMHDGTIAAESDGPGKGTTIVITLPLGHPAAPSAPAAASPA
                     PGPALPHVERRRHVVVADDIEDVAWALSAALQAWGHDVATVADGNALLEQVERDRPEV
                     AIIDLGMPGLGGHEVAQRLRATEHGRGMLLVAVTGWGGDGDRERSLKAGFDAHLVKPV
                     SVESLCALIDGWNP"
BASE COUNT          490 a         1345 c         1261 g          560 t
ORIGIN      
        1 ttcagcttcc tgcgcatcga ccacgtgctg gtcagcccgg acatcggcgt gcgttccgcg
       61 gcgccgggtg gacgcgaagg gtcggagcac cggccggtgg tgacggagct gctcgtcgga
      121 ccgtgacaaa tgccgtgatg acaccgcttg gccgcaacga aatcggcaca gcgggataca
      181 tctcgaaaca agtcaccggc atgacagttg ccaaacccgg cacactcgtt gaaacgaccg
      241 gcaaggagcc cctccatgtc caaggccagg gtgtttggca cgtaccgacc ccgactgaac
      301 atcgccccgc tgctgctgac gcccggattt gcggcaatcg gctacgcggt ccgcggctcc
      361 cagggcgcct ggtgggcggt tgccgcctgg tgcgcggtcg tgctcggcgc cgcggtgtcc
      421 tgcacgctgc acgcctggcg cagtgcccag gaagcgctgc cgcacgccgg ccgccgcgcc
      481 ttcgactacg acgtcaacaa cgccggccgc taggcgggcg acgcgcagcg ccggcttcgg
      541 cgcgcgactg gacgcgacgc gaagatctcc cgtcgcgaac gctccgtcca ttccgggaca
      601 tgcgccttct cggcgcggcc atcccgttct ccgacaaatg ccttgaacgg caagctcaga
      661 acgagaaacg acttgtttcg gcactggaac cggttccgct cgtcctgcgg ctcctgccgg
      721 cctcgtcgcg caccggccac ccgagtcgtt ccgccagcgt cccggccccg cgctgaaagg
      781 acgacgggcc ccgcggggcc cgcctcgagc gatcgctcgc gcgactcagc gctcgttctt
      841 gtggctctgc ttgccggcgt tcgaatgctg ttcgctgctg ccgccacggc tgccttggcg
      901 gtcgctggcc tggttgcccg actgcttgct gccgccgccc tgctggctgc ggctcgacga
      961 accggacgac tgttcctgct gcttctgctg gccagagttg ctgccttgtt gcgaggtcat
     1021 ggagatgctc ctggaggttg accgccggcc ccgtgccggc tttgcaagga cgccgaagcg
     1081 tcacagggcg ggcagcaagc gaaataccta accaggtatg tcgatcggac attccgcgat
     1141 gcggcgtccc accggcgcgc gacttgcccc gctacggttg gcccacggcc cgaggagacg
     1201 cccatgtccg acccgccccg cccgcccggc gccgtgccgc cggtcgagcc ctctccgccc
     1261 gaaccggcgc ccggcgagct cgacgcagaa cggccggacg ggccaccgcg gtcgggcagc
     1321 ggggacaagg agcgcgaatc gcgcccgccc gacgcgcgcg gcggcgctcc ccgccaagct
     1381 tcgcagcttc tcaggggttc cagccgtcga tcaaggcgca gagcgactcg acggacaccg
     1441 gcttgaccag gtgcgcgtcg aagccggcct tgagcgagcg ctcgcgatcg ccgtcgccac
     1501 cccagcccgt gacggccacc agcagcatgc cgcgcccgtg ctccgtggcg cgcaggcgct
     1561 gcgccacctc gtggccgccg agccccggca tgcccaggtc gatgatggcg acctcgggcc
     1621 gatcgcgctc cacctgctcg agcaaggcgt tgccgtcggc cacggtggcg acgtcgtggc
     1681 cccaggcctg cagcgcggcc gagagcgccc aggcgacgtc ctcgatgtcg tcggccacca
     1741 ccacgtggcg gcggcgctcg acgtgcggca gggcgggacc cggcgcgggg ctggccgccg
     1801 ccggcgccga cggtgccgcg gggtgcccca gcggcagggt gatgacgatg gtggtgccct
     1861 tgcccgggcc gtcgctctcg gcggcgatcg tgccgtcgtg catctgcacc acgctgcgcg
     1921 ccaccgacag cccgatgccc atgccctggc cgctgccgac gtcgaactcg ctggcgcgcc
     1981 agaacatctc gaacatggaa cgccgcgcgg cctcgtcgag gcccttgccg tcgtcggcca
     2041 cctcgatgcg gcactggccg ccctcgacgc tggcgcgcag ctcgatgtga ccgccctcgt
     2101 cggtgtactt ggccgcattg atcagcaggt tcgacagcac ctggcccatg cgcgtgggat
     2161 cggcgttgag catgatctcc tcgtcgggca ggcgcacggt cagcgtgtgg ccgcgccggt
     2221 cggcgagcgc gcgcgaggtg tccagcgcgg tctcgacgat ggagcgcagc gtcacgtcct
     2281 gcttcttgag cgccagccgg ccgaagcgca gccgcgagac gtccagcagg tcgtccagca
     2341 gcgcgcgcat cgcctccacc tgcgagcgca gcgtgagctg ggcgcggccc cggtcgacgg
     2401 gcgtcaggcg gcttgcggcc tgcagcgcgt ccgtggcgtt ggcgatggag gcgagcgggt
     2461 tgcgcagctc gtgcgacagc accgccagga agcggtcctt ggcgaggtcg gccgcctgca
     2521 gctggtcgcg cgcctgctcg ttctgcgaca gcgcgtcgac caggtcggcg cggctgcgcg
     2581 cgagttcgtc gcggctctgg cggtcggccg tctggtcgcg caggatcttg acgaagccca
     2641 cgacgtcgcc gcgatggtcg cgcatcggca tcatcacgcc actggcccag aagcgctcgc
     2701 cgttcttgcg ctgcagcgga cgctcgtcgc tcgcccagcc cttggccagc gccgtctcgg
     2761 cctcgctgcg cggcacatcg ccggcgcgct cgtcggtgcc gaagatcttg tcgtagtgct
     2821 ggccgatgat ctcgtcctcg gaccacccca gcaggcgctg cgcgcccacg ctccacgacg
     2881 tgacgcggtg gttggagtcg agcgacagga tcgcgtactc ggtcgcgttg tccatcatca
     2941 ggcgcagccg ttcctcgctc gcgcgcagcg cgtcctcggc ctgcttgcgc acgctgatgt
     3001 ccaggcacac gaccagcacc tcggccggct cgcccggctg cgtctggagc cacgagacgc
     3061 tgttgtgcac ccacagcacg cggccgtcgc ggcacagcag gcgcttctcg atctcgcagc
     3121 tgccctgctc gcgcaggcgc gccagccggg cgtccgactc cgcgcggtcg tcgggatgca
     3181 ccagctcgcg catcggcttg ccgatcagct cgccttcgcc atagcccagc agccggctca
     3241 tgtagcggtt ggagaacgtg atgtcgcccg cctccgaggc ctgcagcacg ccgacggcgg
     3301 ccttgtcggc gatcgcgccg aagcgctgct cggacacccg cagcgcctcc tggttctgct
     3361 ttcgctcggt gatgtccacc agcgtcagca cgatgcctgc gatgcggtcc tcgccgacgc
     3421 ggtacggcag catgcgcacc aggaaccagt gctggcgcgc gtcgttgacc tcgcgctcga
     3481 gcggcaccag gctgctcagc acgcggcgcg cgtcggcatc gagctcgggg tagctcaggt
     3541 ggttgctcag gtccgacagc ggccgaccga cgtcggtcgg gatcaggttg aacagctcga
     3601 ccgccgacgg cgtgtagcgc gtgatgcgca ggctgcggtc catgaagacg gtcgcg
//
LOCUS       DATCRO010000775         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1100385, whole genome shotgun sequence.
ACCESSION   DATCRO010000775 DATCRO010000000
VERSION     DATCRO010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100385"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VIN75_21080"
     CDS             complement(<1..280)
                     /locus_tag="VIN75_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY8976722.1"
                     /translation="MNAPTALRGVERVRHELKRRELTVQAVEAVTPHFRRITLVDDSL
                     SDFVSASFDDHVKVFVGERDATKVRAKGLDGLHRQLAALEFVAHAFDAA"
     gene            386..799
                     /locus_tag="VIN75_21085"
     CDS             386..799
                     /locus_tag="VIN75_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8976723.1"
                     /translation="MDLVHTVMHQFRSTQYQVLRDNAHDVTHMESKVMAFFARHQGAT
                     LSDLVVHSGRDKAQLAKLVKGLRERGLLDAQPDAGDRRSVRLRLSAAGHAVQQALHAE
                     ARVLNERAVDGLSAAEQAQLVALLTRVKTNLGTPE"
     gene            1147..1281
                     /locus_tag="VIN75_21090"
     CDS             1147..1281
                     /locus_tag="VIN75_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976724.1"
                     /translation="MSLSGVALAGWRAMHHKAVSLVRPSALRRAQTRMVRLITVVAGH
                     "
     gene            1397..1951
                     /locus_tag="VIN75_21095"
     CDS             1397..1951
                     /locus_tag="VIN75_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976725.1"
                     /translation="MTQWIVWLELVAEEALVGIALLVVFAGCRRLARDGMSRTPALMV
                     ALGLVLPVGDAWLNMSVIKQVRTLQVEKMAQIGLHGREPAGGWEKAASSPEARTLAST
                     EAARIAYLFQGDHADVVQASGTRTPFQPSADEMRAREQFVRSEKGAESAALSSWDRGI
                     RLLAEAAVFMLAGLAVGWRGRRRA"
     gene            complement(2069..>2825)
                     /gene="fghA"
                     /locus_tag="VIN75_21100"
     CDS             complement(2069..>2825)
                     /gene="fghA"
                     /locus_tag="VIN75_21100"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY8976726.1"
                     /translation="LAGTPVPALFWLAGLTSTEETFAIKAGAQRVASELGLMLVTPDT
                     SPRETGVPGADEHWDFGHGAGFYLNATRSPWAAHWRMESYVTHELPEVIAANFRWRRE
                     ALGLFGHSMGGHGALTLALRHPGLAGSVSAFAPVAAPTHCPWGIKAFANYLGEDSAAW
                     AAHDATELVKSGARAPALLVDQGLSDKFLDEQLNIDRFEAACREAGQSLTLRRHEGYD
                     HGYFFIASFIEDHLRFHADALASLGRRAGQPQA"
BASE COUNT          403 a          957 c         1029 g          436 t
ORIGIN      
        1 gcgccgcgtc gaacgcgtgc gccacgaact caagcgccgc gagctgacgg tgaaggccgt
       61 cgaggccctt ggcccgcact ttcgtcgcat cacgctcgcc gacgaagacc ttgacgtggt
      121 cgtcgaagga cgcgctgacg aagtccgaca gcgagtcgtc cacgagcgtg atgcgccgga
      181 agtgcggcgt gacggcctcg acggcctgca ccgtcagttc gcggcgcttg agttcgtggc
      241 gcacgcgttc gacgccgcgc agggcggtgg gagcgttcat ggcgggatcg ttcgatttgg
      301 ttgacaatgt catccaatca tgtggaaaaa agtagacaat gtcaaccatg tgggttctgg
      361 ccccgggtcg ccggccgagg ccgtgatgga cctcgtccac acggtgatgc accagttccg
      421 ctcgacgcag taccaggtgc tgcgcgacaa cgcgcacgac gtcacgcaca tggagagcaa
      481 ggtgatggcg ttcttcgcgc gccaccaggg cgcgacgctg agcgacctgg tcgtccacag
      541 cggccgcgac aaggcgcagc tggccaagct ggtcaagggc ctgcgcgagc gcggcctgct
      601 cgacgcccaa ccggacgccg gcgatcgccg cagcgtgcgc ctgcggctca gcgccgcggg
      661 ccacgcggtg cagcaggcgc tgcacgccga ggcgcgcgtg ctcaacgaac gcgccgtcga
      721 cggactgagc gcggcggagc aggcccagct cgtcgcgttg ctgacccggg tcaagacgaa
      781 cctcggcacc ccggaatagg ctcgtccctg gcccgccgac gcggcactgt ggattgaaac
      841 acaccgctgg cgccaggggc ttgcattggc gccgggatca cgtagactgt tcattcatac
      901 agtatccgtg atggcttcga tctcgcgctc gccatggacg tccggcactc tctcaatctc
      961 ctgcctggcc ctggcgaacg cgaggccctt ggcgatgggg cggcgtgatg gcacgtttcc
     1021 tcgtctcgcc cggcgcggtg tatgcgctga cggtgctcct gtcgttgctg ttcgcttgtc
     1081 tggtcgtgtg ggcgcgcgag tgcccgtgaa ggagtcgtca tgtcggcgtg gctgtcccac
     1141 caatccatga gcctgtcggg cgtcgccctg gcgggctggc gcgcgatgca tcacaaggcg
     1201 gtgtcgctgg tgcgcccgtc cgcgctgcgc cgcgcgcaga cgcggatggt gcggctgatc
     1261 acggtggtgg cgggccactg accgcccgcc tggccgacgc ggccgccact tcggggcgag
     1321 gcccgcgtcc gccgtcgggc cgaaagcgcc cgacaatcgc gccttcttga atgggaatgc
     1381 ggaaggcgcc tgatcgatga ctcaatggat tgtctggctc gaactcgtgg ccgaagaggc
     1441 gctcgtcggt atcgccctgc tggtcgtgtt cgcggggtgc cggcgactgg cgcgtgacgg
     1501 catgtcgcgc acgccggcgc tcatggtggc gctcgggctc gtgctgccgg tgggcgatgc
     1561 ctggctcaac atgagcgtca tcaagcaggt gcggacgctg caggtcgaga agatggcgca
     1621 gatcgggctg catggccgcg agcccgccgg tggctgggag aaggcggcat cgtcgcccga
     1681 ggcgcgcacg ctggccagca ccgaagccgc gcgcatcgcc tacctgttcc agggtgacca
     1741 cgccgacgtc gtccaggcct ccggcacccg cacgccgttc cagccgtccg ccgacgagat
     1801 gcgcgcacgc gagcagttcg tgcgcagcga gaagggcgcc gagtccgcgg cactgtcgtc
     1861 ctgggatcgc ggcatccgcc tgctggccga ggcggcggtc ttcatgctgg ccggcttggc
     1921 cgtcggctgg cgcgggcgcc ggcgtgcgtg aggcgcgggg atgcctggca gggcgggttg
     1981 gccgcgggct tgacccgcca cctccccgcg tccggaggcc cggcgttctt ggccgcagag
     2041 agggatcagt gcgccgacga acggcgggtc acgcctgcgg ctgacccgcc ctacggccca
     2101 ggctggccaa cgcatccgcg tggaaccgca ggtgatcctc gatgaagctc gcgatgaaga
     2161 agtagccgtg gtcgtagcct tcgtggcggc gcagcgtcag cgattggccg gcctcccggc
     2221 aggcggcctc gaagcggtcg atgttcagtt gctcgtccag gaacttgtcg ctgaggccct
     2281 ggtcgaccag cagcgccggc gcgcgagcgc ctgatttcac cagctcggtg gcgtcgtgcg
     2341 cggcccaggc cgcgctgtcc tcgcccagat agttcgcgaa cgccttgatg ccccaggggc
     2401 aatgcgtggg cgcagccacc ggcgcgaagg ccgacacgct gccggccagc cccgggtggc
     2461 gcagggccag cgtcagcgcg ccgtggccgc ccatcgaatg gccgaacaac ccgagcgcct
     2521 cgcggcgcca gcggaagttg gcggcgatga cctcgggcag ctcgtgcgtg acgtagcttt
     2581 ccatgcgcca gtgcgcggcc cacggcgagc gcgtggcgtt caggtagaag cccgcgccgt
     2641 ggccgaagtc ccagtgctcg tcggcgcccg gcacgccggt ctcgcgcggg ctcgtgtcgg
     2701 gcgtgacgag catcagcccg agttcggacg ccacgcgctg cgcgccggcc ttgatcgcga
     2761 aggtctcctc ggtggacgtc agccccgcca gccagaagag cgccggcacg ggcgtgccgg
     2821 ccagc
//
LOCUS       DATCRO010000776        13157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_998870, whole genome shotgun sequence.
ACCESSION   DATCRO010000776 DATCRO010000000
VERSION     DATCRO010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998870"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VIN75_21105"
     CDS             complement(<1..756)
                     /locus_tag="VIN75_21105"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8976727.1"
                     /translation="MWRRPLCVFLASTALAVAPAVAGIVAIGPGSPPGAGGMIVTAEI
                     TPGDASSAFLNLDFTAMAPIDVAIGTSSASPIATYGSAINDTASTWTGFTVRVVDGTT
                     SFLALNDPNDPYGSYSDLPGWNVALSSDGRTLTFSGGSIAPGGSLDQVLGLSVAGATG
                     AVTLAFAAVVPEPATGALCLLGLGLLARALRRTRARGATTLLPALAAAALPAHAAGPQ
                     VVADWQAQLAITPVGSVAGLPTQMAFGPDGRLYV"
     gene            complement(915..2129)
                     /gene="gspF"
                     /locus_tag="VIN75_21110"
     CDS             complement(915..2129)
                     /gene="gspF"
                     /locus_tag="VIN75_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY8976728.1"
                     /translation="MPAFKYEALDANGKSSTGIVEAESAKAARSQLRSLQLVPLGLTP
                     VAVVESAEKRSPFARKTFSSSSLAVWTRQLAGLVAAGLPLERALTALADEAEEQKQRD
                     LIAHLRSEVNGGSTFARALGSVPREFDEVYRAVVAAGEQSGALGKVLERLADDLEERQ
                     SLKGKLIGAMLYPAIVSVIAVIIVTFLVTYVVPQVASVFASSKHALPFLTSMMLSISA
                     FVRQWGLLAAALLVAGGILFTAALRQPGFRLRFDATFLNLPLAGRLARGYNAARFAST
                     LAMLAGAGVPILKALQAAAETLGNHALRADAMEALVQVREGAPLASALAAHKRFPGLL
                     AMFARLGEQTGQLPVMLERAARQLANEVQRRAFALATLLEPLLIVAMGGVVLVIVLSV
                     LLPIIQLNDFVK"
     gene            complement(2231..3652)
                     /gene="gspE"
                     /locus_tag="VIN75_21115"
     CDS             complement(2231..3652)
                     /gene="gspE"
                     /locus_tag="VIN75_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY8976729.1"
                     /translation="MGARHPLPYAWAKTYSVLIEDDGRERVLHVSDGTSRQALAEVLR
                     LHAIDRIESEAPAALQTRIAQAYSGGQGSAASVVGEVESAVDLSRMMQELPAVEDLLE
                     ASNDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAA
                     LISRLKIMAELDISEKRLPQDGRISLRIGGRAIDVRVSTLPSAHGERAVLRLLDKGEQ
                     KFSLEGLGMSGDTLDKFNRLVQQPHGIVLVTGPTGSGKTTTLYASLTRLDTRTSNVLT
                     VEDPVEYELAGIGQTQVNPKIDLTFAKALRAILRQDPDVVMIGEIRDFETAQIAIQAS
                     LTGHLVLATVHTNDAPSTVARLIDMGVEPFLLSSSLLGVLAQRLVRKLCEYCKREDEH
                     HRWHPVGCEHCANSGYKGRTGVYELMVVDETVQSLIHGRAAEQDLIKAAIANGMRSMR
                     EDGERLVAEGITSLEEVVRVTRD"
     gene            complement(3671..6088)
                     /gene="gspD"
                     /locus_tag="VIN75_21120"
     CDS             complement(3671..6088)
                     /gene="gspD"
                     /locus_tag="VIN75_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY8976730.1"
                     /translation="MERTVTSASRLHATSIAIALLAAASTLAQAAPRKGPAAPASDAA
                     PDDAAVPTSRARGTPVTLNFVNADIEAVSRAIGAMLNRDILVDPRVKGNITVYNDKPQ
                     PLGEAYRNYLSALRGLGFTVVESGGFLKVVPEADAKLQTSTVSVGAPSQRGDVVLTQI
                     FKLNYENPNNLVAVLRPLITANNTINASPGNNSLVITDYADNLQRLGKIISALDQPAG
                     GEMQVVQLKNAVASDIVQTVQKLLDSSNSAVPGVPGQSSAASVIVEPRSNSLIVRATN
                     QSRMAAALSIIDKLDQPVAGGGPAGNIWVVYLKNADAVKVAELLRAAVSANSGGNGTG
                     GGSPTGGTGGASTSEGRTSMTQNPNAPSGTSGTAGGASAQSTTPVAASARPSTGGQVQ
                     ADPATNSLIITASEPVYRQMRQVIDQLDVRRAQVYVESMMVSVDATKAAQAGIQWQGL
                     FGAGTSYVGGGTNFSSSSGAGNIINLAVAGGQLSSGTTTGTGSTSSTAVPSANVSAGL
                     NVGFVPKINGIYTLAALANFLETNAGGNVLATPNIVALDNEEAKIVIGQNIPLTTGSY
                     TNTGGNSSTVNPFQTVERQDIGITLRIRSQIGENGTIRMTVYEENSARDPNFATNQIT
                     TKTSVETYITVDDGAMVVLGGLTRDEYGDTSDGVPLLSSIPIIGNLFKSSTRTRHKQT
                     LMLFLRPIVMRDQSDVNKLTVDRYNAIRQQQQSFQPDTSWVMRINDTPVVPPVKPDTQ
                     IDPNVPHDPETESVKLPTITPVPDMRRVPAPPVAPLIPVKPAPNPPQGTTLPSAPPPA
                     PAASQGQ"
     gene            complement(6091..7074)
                     /gene="gspN"
                     /locus_tag="VIN75_21125"
     CDS             complement(6091..7074)
                     /gene="gspN"
                     /locus_tag="VIN75_21125"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY8976731.1"
                     /translation="MSIVRLRSDRAPRGFAPSRFSRPPEEGQVRLLRRQRSLAIVGAL
                     VGLVIGSIAFLPASLLAGAVASATNDQFLLAEAEGTVWHGSAITVLTGGAGSHDASVL
                     PTRLEWTLHPRWNGLSLHLTQDCCLAHGLDLNLRRTIDAWNVEVIGPDERDQPTVGPT
                     KAMPGEYGADAMILDSATPVGQWPMGWLEGRGFPWNTIHPGGVLTLSTHNLRFTLQGG
                     HWRTLGSAQVVIRQASSRLTTLDSLGTYRVLIQPDPSTQVKPGEGASRSLVWISTIDG
                     ALMIDGRGLIGATGFRLRAEAHAAPGNEAALDNLLNLIGRRNGASSAISIG"
     gene            complement(7115..7606)
                     /gene="gspM"
                     /locus_tag="VIN75_21130"
     CDS             complement(7115..7606)
                     /gene="gspM"
                     /locus_tag="VIN75_21130"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY8976732.1"
                     /translation="MSALSDTRNQLLARYAKLDARERQMVVIIAVALTFLIVWLVLVR
                     PALNTLETAPAQRDQADAQLLQMQAISNEARQLRALPPVPPSVAEQVLKSATDELGGK
                     AKIQMQGDRATLSVTGINGEELRKWLIQARGGARARPIEANLVRAGDGYNGTLVVSIG
                     SQQ"
     gene            complement(7603..8853)
                     /gene="gspL"
                     /locus_tag="VIN75_21135"
     CDS             complement(7603..8853)
                     /gene="gspL"
                     /locus_tag="VIN75_21135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY8976733.1"
                     /translation="MSTLIILLAAQPRLADPSAAGHGAPDEFDYVLSVDGQRMTDRGR
                     APASELPRAALVVAVLRPADVAFQRVAVPKAPAARMRAALVGVMEEALLEDEDEVHLA
                     LAPDAKPGGEHWVAVVNKPWLRAQLDALEAGDVPLDRAVPSWWPDEGVAGHTYREGGP
                     DEPAQLAWRDDAGVLCVPLQTPVARALLTGLAEDTVVRWTATPDTALDASEFVGMPVA
                     TVTDEEQALRAARAGWNLLQFDLAPQRRGVKLARDLAVRFAFDPAWRVTRRGLLALVV
                     VSLLGLNVRALQESHGVAQKKAALTAAVKEAFPALKPIYDPAGQAQQQIDLLRAAAGK
                     PGDGDIETLLQAAESAWPEGKSPIENLKFEPGRLTLPATGWTPQEIERFTTQLRASNV
                     DVRSDTGKLQLSRRQPSAGTSKKQ"
     gene            complement(9060..10109)
                     /gene="gspK"
                     /locus_tag="VIN75_21140"
     CDS             complement(9060..10109)
                     /gene="gspK"
                     /locus_tag="VIN75_21140"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY8976734.1"
                     /translation="MTPHPTVRRQRGAALLLALLIMTLVATLAAGMVWLQWRGIEVET
                     AERARAQAQWLLNASLDWGNLILKSSIRQGHTEDDLGQPWATPLAETKLSSFLSADGS
                     HAEDSGPEAYLSGQITDAQSKYNLYNLTLPPDGQQLGKKTEIERLQLLFQAIGVSLDV
                     ANTIALGMRASQDAQQAAANPGTQVDPNSILMPIEVRDLAWYGVDPKVLKQIEPFVQI
                     IPFSPQQAAGNGYPQMPINANTAPAEVLMAGCPGLSRAQAEQLILQRRQQPFNDAGRL
                     LQALKGGGTAPLPCEATNGHPGDILTIKSDHFEIYGQLRYEQHVIRERSVIYRKDQFT
                     PVQVLRRERLPPDAS"
     gene            complement(10111..10773)
                     /locus_tag="VIN75_21145"
     CDS             complement(10111..10773)
                     /locus_tag="VIN75_21145"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8976735.1"
                     /translation="MVRRSRPSGFTLVEVMVALVIMAVLSALAMRGMDALMRAKDAAL
                     ASTDRTLKLNTGMSQFEYDMSMVVDSKVLPKAIMFDGATLRIARRSPQGIQLVLWTLQ
                     DHRWQRWASAPVVHMSELTDAWMRSQQWTAISGGAITVLENVDDFQVYACNPAAVNTP
                     GCSWNNIQSTQGAAQNNPPACTQPPCTPPPAPPQPDGIKIVLKVPEGEIMRERQLPRF
                     GG"
     gene            complement(10773..11144)
                     /gene="gspI"
                     /locus_tag="VIN75_21150"
     CDS             complement(10773..11144)
                     /gene="gspI"
                     /locus_tag="VIN75_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY8976736.1"
                     /translation="MTSRPRPRAAGFTLIEVMVALAIVAIALAAGSRAASSVVDTSQR
                     LSDVVLGQWCADNQLTDMRLLDQMPPVGKTPFACTQLGRDFKGTLVVQGTPNPSFRRV
                     DATVADTDGRQLVTISTVMGH"
     gene            complement(11141..11668)
                     /locus_tag="VIN75_21155"
     CDS             complement(11141..11668)
                     /locus_tag="VIN75_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8976737.1"
                     /translation="MCSASAPTGSPVVKATTRTSAPGSRPRRSTAGFTLLELMVVVLI
                     VAVASGMISLSLRDRSQSKLETEGARLAALLETARTQSRIIGTDVRWEPVAAGGFEFT
                     GLPPKAAKELPHAWLESDTKASIVGAPQLKLGPEPLLPAQRVVLHLGDRDLAVGTDGL
                     SPFQIIDSATGAPAP"
     gene            complement(11595..12029)
                     /gene="gspG"
                     /locus_tag="VIN75_21160"
     CDS             complement(11595..12029)
                     /gene="gspG"
                     /locus_tag="VIN75_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8976738.1"
                     /translation="MNQALIARRHSRGFTLLELMVVIVIIGVLAALIAPNVLSRVAQA
                     KITAAHSDINSLMNALKIYKLDNGRYPSTDQGLQALVSKPTTGSIPGNWKSYLDKLPE
                     DPWHHPYQYANPGTHGEIDVFSFGADGQPGGEGDDADIGSWE"
     gene            12080..12796
                     /locus_tag="VIN75_21165"
     CDS             12080..12796
                     /locus_tag="VIN75_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY8976739.1"
                     /translation="MSSRWTGFFVWALVAACTAFWAMKIFAATRPVPPTAQAPQVAAA
                     NGPMVRLFGAVVVAPPTVNAPPPESSRFQLVGVIAPPAGTAQGGYAVVSVDNQPARTW
                     RVGANIEGNTSLLSVSKRGAEFGPPGGPTSFSLQLPEPAAAETGTLQPAVSQPDPAQL
                     QQQQLQQAQPQMQPQQQMQPQMQQPNVQPSMRGGFGRAASLAGRRGFQGPQGNNGTMP
                     PKMGVPPDQQTQNDSGGVQQ"
     gene            complement(12848..>13157)
                     /locus_tag="VIN75_21170"
     CDS             complement(12848..>13157)
                     /locus_tag="VIN75_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8976740.1"
                     /translation="GNAELHRVGLERFFAGSVSARLHGVAKPDPSIFRAACEAAGAAP
                     AQVLHLGDDLDADVGGALAAGLHAGWICRPDGAHASADPRAGAHRFADLVEVALALGV
                     "
BASE COUNT         1773 a         4522 c         4840 g         2022 t
ORIGIN      
        1 cacgtacagc cggccgtcgg gaccgaaggc catctgcgtg ggcaggcccg cgacgctgcc
       61 caccggcgtg atggccagtt gcgcctgcca gtcggccacg acctgcggcc cggcggcgtg
      121 cgccggcagc gccgcggcgg ccagcgcggg cagcagcgtc gtggcgccgc gggcgcgggt
      181 gcggcgcagc gcgcgcgcga gcaggcccag cccgagcagg cacagtgcgc cggtggcggg
      241 ctcgggcacc accgccgcga aggcgagcgt gacggcgccg gtcgcgcccg cgacgctcag
      301 gcccagcacc tggtccagcg agccgccggg tgcgatgctg ccgcccgaga acgtcagcgt
      361 gcggccgtcg ctgctcaacg cgacattcca gcccggcagg tcgctgtagc tgccgtacgg
      421 gtcgttcggg tcgttcagcg cgaggaagct cgtcgtgccg tcgaccacgc gcaccgtgaa
      481 gccggtccat gtcgacgcgg tgtcgttgat cgcgctgcca tacgtggcga tgggcgatgc
      541 gctcgacgtg ccgatggcca cgtcgatcgg cgccatggcg gtgaagtcga ggttcaggaa
      601 cgcggaactc gcgtcacccg gcgtgatctc cgcggtgacg atcatgccgc ccgcgcccgg
      661 cgggctgccg ggcccgatcg ccacgatgcc ggcgaccgcg ggagcgacgg ccagggctgt
      721 cgacgccagg aacacgcaca agggacgccg ccacatggtc cacctcgcct ctggggttgt
      781 tcaacgttcg agccggcctc cgggggcagg cccgcgaaac gttatcccaa tcgatggggg
      841 cgagaaacac aacgctcgct atcggcggca ggccgtcgag cgtggcgttt actactgctc
      901 gacgtgttcg ccgctcactt gacgaagtcg ttgagctgga tgatgggcag cagcaccgac
      961 agcacgatga cgagcacgac gccgcccatc gcgacgatga gcagcggctc gagcagcgtg
     1021 gccagcgcga acgcgcggcg ctgcacctcg ttggcgagct ggcgcgcggc gcgctcgagc
     1081 atcacgggca gctggccggt ctgctcgccc agccgggcga acatggccag caggcccggg
     1141 aagcgcttgt gcgcagccag cgcggacgcg agcggcgccc cctcgcgcac ctgcaccagc
     1201 gcctccatcg cgtcggcgcg cagcgcgtgg ttgcccaggg tctcggcggc ggcctgcagc
     1261 gccttgagga tcggcacgcc cgcgccggcc agcatggcca gggtgctcgc gaagcgggcc
     1321 gcgttgtagc cgcgcgccag gcgcccggcc agcggcaggt tcaggaacgt ggcgtcgaag
     1381 cgcaggcgaa agcccggctg gcgcagcgcc gcggtgaaca ggatgccgcc ggcgaccagc
     1441 agcgccgcgg ccagcaggcc ccattgccgc acgaaggcgc tgatggacag catcatcgac
     1501 gtgaggaagg gcagcgcgtg cttgctgctc gcgaacaccg acgccacctg cggcacgacg
     1561 taggtcacca ggaacgtgac gatgatgacc gcgatcaccg acacgatggc cgggtacagc
     1621 atcgcgccga tcagcttgcc cttgagcgac tggcgctcct ccaggtcgtc ggccaggcgc
     1681 tcgagcacct tgcccagcgc gccgctctgc tcgcccgcgg ccacgaccgc gcggtagacc
     1741 tcgtcgaact cgcgcggcac gctgcccagc gcgcgggcga aggtcgagcc gccgttgacc
     1801 tcgctgcgca ggtgcgcgat caggtcgcgc tgcttctgct cctcggcctc gtcggccagc
     1861 gcggtcagcg cgcgctccag cggcagcccc gccgcgacca ggccggccag ctggcgcgtc
     1921 cacacggcca ggctggacga cgagaacgtc ttgcgcgcga acggcgagcg cttctcggcc
     1981 gactcgacca ccgccaccgg cgtcaggccc agcggcacca gttgcaggct gcgcagctgc
     2041 gagcgcgcgg ccttggccga ctcggcctcg acgatgccgg tggacgactt gccgttggcg
     2101 tcgagcgcct cgtacttgaa tgcaggcatg ggtgtctgta cttcgaaccc tcagccggcg
     2161 ccgggccgcc ccagggcggc tgcgccccct cggggggcag caagcagggt gagcgtgggg
     2221 gccaacatgt tcagtcgcgc gtcacgcgga ccacttcttc cagcgacgtg atgccttcgg
     2281 cgaccagccg ctcgccgtcc tcgcgcatcg agcgcatgcc gttggcgatc gcagccttga
     2341 tgaggtcctg ctccgcggcg cggccatgga tcagcgactg caccgtctcg tccaccacca
     2401 tcagctcgta gacgcccgtg cggcccttgt agccggaatt ggcgcaatgc tcgcagccca
     2461 cggggtgcca gcggtggtgc tcgtcctcgc gcttgcagta ctcgcagagc ttgcgcacga
     2521 ggcgctgcgc cagcacgccc agcagcgagg agctgagcag gaacggctcg acgcccatgt
     2581 cgatcaggcg cgcgacggtg gacggcgcgt cgttggtatg caccgtcgcg agcaccaggt
     2641 ggcccgtcag cgaggcctgg atggcgatct gcgcggtctc gaagtcgcgg atctcgccga
     2701 tcatcacgac gtccggatcc tggcgcagga tcgcgcgcaa cgccttggcg aacgtgaggt
     2761 cgatcttcgg gttgacctgc gtctggccga tgcccgcgag ctcgtattcg accgggtcct
     2821 ccacggtcag cacgttggag gtgcgggtgt ccaggcgcgt cagcgaggcg tacagcgtgg
     2881 tcgtcttgcc ggaaccggtc gggcccgtga cgagcacgat gccgtgcggc tgctgcacga
     2941 ggcggttgaa cttgtcgagc gtgtcgccgc tcatgcccag cccctcgagg ctgaacttct
     3001 gctcgccctt gtccagcaga cgcagcaccg cgcgctcgcc gtgcgcggac ggcagcgtcg
     3061 agacgcgcac gtcgatcgcg cggccgccga tgcgcagcga gatgcggccg tcctgcggca
     3121 ggcgcttctc ggagatgtcg agctcggcca tgatcttcag tcgcgagatc agcgccgcgt
     3181 gcagcgcctt gttcggctgc accacctcgc gcagcgtgcc gtcgacgcgg aagcgcacgc
     3241 tggagctgcg ttcgtagggc tcgatgtgga tgtcgctcgc gccgtccttg gcagcctgcg
     3301 tcagcagcgc gttgagcatg cggatgatcg gcgcgtcgtt gctggcctcc agcaggtctt
     3361 cgaccgccgg cagctcctgc atcatgcgcg acaggtcgac cgcgctttcc acttcgccga
     3421 ccaccgacgc cgcgctgccc tggccgcccg agtaggcctg cgcgatgcgg gtctgcagcg
     3481 ccgcgggcgc ctcgctctcg atgcggtcga tcgcgtgcag gcgcagcacc tcggcgagcg
     3541 cctggcgcga cgtgccgtcg ctcacgtgca gcacgcgctc gcggccgtcg tcctcgatca
     3601 gcaccgaata cgtcttggcc caggcgtagg gcaggggatg gcgtgcgccc atgatcggac
     3661 ctccggtgcc ttactggcct tgcgacgcgg cgggcgccgg cggcggcgcg ctgggcagcg
     3721 tcgtgccctg cggcgggttc ggcgccggct tgaccgggat cagcggcgcc accggcgggg
     3781 ccggcacccg gcgcatgtcg ggcaccggcg tgatggtggg cagcttgacc gattcggtct
     3841 ccgggtcgtg cggcacgttc gggtcgatct gcgtgtcggg cttgaccggc ggcacgaccg
     3901 gcgtgtcgtt gatgcgcatg acccagctcg tgtccggctg gaagctctgc tgctgctggc
     3961 ggatcgcgtt gtagcggtcg acggtcagct tgttcacgtc gctctggtcg cgcatgacga
     4021 tcgggcgcag gaacagcatc aacgtctgct tgtggcgcgt gcgcgtcgag ctcttgaaca
     4081 ggttgccgat gatcgggatc gacgacagca gcggcacgcc gtcgctggtg tcgccgtact
     4141 cgtcgcgcgt caggccgccc agcaccacca tcgcgccgtc atcgacggtg atgtaggtct
     4201 ccaccgaggt cttggtggtg atctggttgg tggcgaagtt cgggtcgcgc gccgagttct
     4261 cctcgtacac cgtcatgcgg atggtgccgt tctcgccgat ctggctgcgg atgcgcagcg
     4321 tgatgccgat gtcctggcgc tcgaccgtct ggaacgggtt gacggtgctg gagttgccgc
     4381 cggtgttggt gtacgagccc gtcgtcaggg ggatgttctg gccgatgacg atcttggctt
     4441 cctcgttgtc cagcgccacg atgttgggcg tggccagcac gttgccgccg gcgttggtct
     4501 cgaggaagtt ggccagcgcg gccagcgtgt agatgccgtt gatcttgggc acgaagccca
     4561 cgttcaggcc ggcgctgacg ttggccgacg gcacggcggt cgagctggtc gagcccgtgc
     4621 cggtggtggt gccgctggac agttgcccgc ccgcgaccgc gaggttgatg atgttgccgg
     4681 ccccgctgga gctgctgaaa ttggtgccgc cgcccacgta ggaggtgccg gcgccgaaca
     4741 ggccctgcca ctggatgccg gcctgcgccg ccttggtggc gtcgacgctg accatcatcg
     4801 actcgacata gacctgcgcg cggcgcacgt ccagctggtc gatgacctgg cgcatctggc
     4861 ggtacaccgg ttccgacgcc gtgatgatca gcgagttggt ggccggatcg gcctgcacct
     4921 ggccgcccgt tgacggacgc gccgaggccg ccaccggcgt cgtcgactgc gcggacgcgc
     4981 cgccggccgt gcccgacgtg cccgagggcg cgttcgggtt ctgcgtcatg gaggtgcggc
     5041 cttcggatgt cgacgccccg ccggtgccgc cggtcgggct gccgccgccg gtgccgttgc
     5101 cgccgctgtt ggccgacacc gccgcgcgca gcagctcggc caccttcacg gcatcggcgt
     5161 tcttcaggta gacgacccag atgttgccgg cgggcccgcc gccggccacc ggctggtcga
     5221 gcttgtcgat gatggacagc gccgcggcca tgcgcgactg gttggtggct cgcacgatca
     5281 gcgagttgct gcgcggctcg acgatgaccg acgccgcgct gctctggccg ggcacgcccg
     5341 gcaccgcgga gttcgagctg tccagcagct tctgcaccgt ctggacgatg tccgaggcga
     5401 cggcgttctt cagctgcacg acctgcatct cgccgccggc gggctggtcg agcgccgaga
     5461 tgatctttcc caggcgctgc aggttgtcgg cgtagtcggt gatgaccagc gagttgttgc
     5521 ccgggctcgc gttgatcgtg ttgttggcgg tgatcagcgg gcgcagcacg gccacgaggt
     5581 tgttcgggtt ctcgtagttg agcttgaaga tctgcgtcag cacgacgtcg ccgcgctgcg
     5641 acggcgcgcc cacggagacg gtgctggtct gcagcttcgc gtcggcctcg ggcaccactt
     5701 tcaggaagcc gcccgactcc acgacggtga agcccagccc gcgcagcgcg gacaggtagt
     5761 tgcgataggc ctcgcccagc ggttgcggct tgtcgttgta gacggtgatg ttgcccttga
     5821 cgcgcgggtc gaccaggatg tcgcggttga gcatggcgcc gatggcgcgc gagacggcct
     5881 cgatgtcggc gttgacgaag ttcagcgtga ccggcgtgcc gcgggcgcgc gatgtcggca
     5941 ccgcggcgtc gtccggcgcg gcgtcgctcg cgggggccgc agggcccttg cgcggggcgg
     6001 cctgggcgag cgtggaggcg gcggcgagca gggcgatcgc gatcgacgtc gcgtgcaggc
     6061 gggaggcaga agtgacggtt cgttccatga tcacccgatc gagatggcgg acgacgcacc
     6121 gttgcggcgt ccgatgagat tcagcaagtt gtcgagggcg gcctcgttgc cgggggccgc
     6181 atgcgcctcc gcccgcaacc ggaagccggt ggcgccgatc agcccgcggc cgtcgatcat
     6241 cagggcgccg tcgatggtcg agatccagac cagcgagcgc gaggcgccct cgcccggctt
     6301 gacctgcgtg ctcggatccg gctggatcag gacgcggtag gtgcccaggg agtcgagcgt
     6361 cgtcaggcgc gacgacgcct ggcgaatcac cacctgcgcg ctgccgagcg tgcgccaatg
     6421 cccgccctgc agcgtgaagc gcaggttgtg cgtcgacagc gtcagcacgc cgcccgggtg
     6481 gatcgtgttc cacgggaagc cgcggccctc gagccagccc atcggccatt ggcccacggg
     6541 ggtggcggag tccaggatca tcgcgtcggc gccgtattcg ccgggcatcg ccttggtggg
     6601 cccgacggtg ggctggtcgc gctcgtcggg gccgatgacc tcgacgttcc aggcgtcgat
     6661 cgtgcggcgc aggttcaggt cgaggccatg cgcgagacag cagtcctggg tcaggtgcag
     6721 cgacaggccg ttccagcgcg ggtgcagcgt ccactccagg cgcgtgggca ggaccgacgc
     6781 atcgtggctg ccggcgccgc cggtcagcac ggtgatggcg ctgccgtgcc agaccgtccc
     6841 ctcggcctcg gccagcagga actggtcgtt ggtggcgctg gccaccgcgc cggccagcag
     6901 gctcgcgggc aggaaggcga tgctgccgat cacgaggccg acgagcgcgc cgacgatcgc
     6961 cagcgagcgc tggcgccgca gcagccgcac ctggccttcc tcgggcggcc gcgagaaccg
     7021 cgagggcgcg aacccccgcg gggcccggtc cgagcgcagc cggacgatgc tcatgcgcgg
     7081 cgtcctcctg cgatggtgga tgcggatgcg gccatcactg ctgcgagccg atggacacga
     7141 ccagcgtgcc gttgtagccg tcgccggcgc gcaccaggtt ggcctcgatc ggacgcgcgc
     7201 gcgcgccgcc gcgggcctgg atcagccact tgcgcagctc ctcgccgttg atgccggtga
     7261 ccgacagcgt cgcgcggtcg ccctgcatct ggatcttggc cttgccgccg agttcgtcgg
     7321 tggccgactt gagcacctgc tcggccaccg agggcggcac cggcggcagc gcgcgcaact
     7381 gcctggcctc gttggagatc gcctgcatct gcagcagctg cgcgtcggcc tggtcgcgtt
     7441 gcgccggcgc ggtctccagc gtgttcaggg ccggccgcac gagcacgagc cagacgatca
     7501 ggaacgtcag ggcgacggcg atgatcacca ccatctggcg ctcgcgcgcg tcgagcttgg
     7561 catagcgcgc cagcagctgg ttgcgggtat cggagagggc gctcattgtt tcttgctcgt
     7621 tccggccgac ggctggcggc ggctcagctg cagcttgccg gtgtccgacc gcacgtcgac
     7681 gttcgaggcg cgcagctggg tggtgaagcg ttcgatctcc tgcggcgtcc agccggtggc
     7741 cggcagcgtc aggcggccgg gctcgaactt caggttctcg atcggcgact tgccttccgg
     7801 ccatgccgac tcggccgcct gcagcagcgt ctcgatgtcg ccgtcgccgg gcttgccggc
     7861 ggccgcgcgc agcaggtcga tctgctgctg cgcctggccg gcggggtcgt agatcggctt
     7921 gagggcgggg aacgcttcct tgaccgcggc ggtgagcgcg gccttcttct gcgcgacgcc
     7981 gtggctctcc tgcagcgcgc gcacgttcag gcccagcagg ctcacgacga caagggccag
     8041 cagcccgcgc cgggtgacgc gccaggccgg gtcgaacgcg aagcgcacgg cgaggtcgcg
     8101 cgcgagcttg acgccgcggc gctggggcgc gaggtcgaac tgcaacaggt tccagccggc
     8161 gcgcgcggcg cgcagcgcct gctcctcgtc ggtgaccgtg gccaccggca tgccgacgaa
     8221 ctcgctcgcg tcgagcgcgg tgtcgggcgt ggccgtccag cgcacgacgg tgtcctcggc
     8281 caggcccgtg agcagggcgc gggccaccgg tgtctgcagc ggcacgcaca gcacgccggc
     8341 gtcgtcgcgc cacgcgagct gcgccggctc gtccgggccg ccctcgcggt aggtatggcc
     8401 ggccacgccc tcgtcgggcc accacgacgg cacggcgcgg tccagcggca cgtcgccggc
     8461 ctcgagcgcg tcgagctgcg cgcgcagcca cggcttgttg acgacggcga cccagtgctc
     8521 gccgccgggc ttggcgtcgg gcgccagcgc caggtgcacc tcgtcctcgt cctcgagcag
     8581 cgcctcctcc atcacgccca ccagcgcggc gcgcatgcgg gccgcgggcg ccttggggac
     8641 ggccacgcgc tggaacgcga catcggccgg acgcagcacc gccacgacca gcgcggcgcg
     8701 cggcagctcc gacgccggcg cgcggccacg atcggtcatg cgctggccgt cgacgctcaa
     8761 cacgtagtcg aactcgtcgg gcgcgccgtg cccggcggcc gacgggtcgg cgagccgggg
     8821 ctgggctgcg agcaggatga tcagcgtgga catggatttg tggcgaattt accgcctgcg
     8881 cgtgaacgcg ggcgacgccc cgtgcagcag ggctgtgcag ggagcaaaat gggcgtattg
     8941 tgacctgtgg cgatgacgcc accggcgact ttctgtcaca cggcgctgcg ggaaaacgcc
     9001 agccccgaaa ccggcgtcgg gcgggcaatc ccccgttccc ggtatccgtg cgccgccgtt
     9061 catgaggcgt cgggcggcag gcgttcgcgg cgcaggacct gcaccggggt gaactggtcc
     9121 ttgcgataga tgaccgagcg ttcgcggatc acgtgctgct cgtagcggag ctgcccgtag
     9181 atctcgaagt ggtccgactt gatggtcagg atgtcgcccg gatgaccgtt cgtggcctcg
     9241 cagggcaggg gcgcggtgcc gccgcccttg agcgcctgca ggagccgccc cgcatcgttg
     9301 aagggctgct ggcgccgttg caggatcagc tgctcggcct gcgctcgcga cagccccggg
     9361 cagccggcca tcagcacctc ggccggcgcc gtgttggcgt tgatcggcat ctgtgggtag
     9421 ccgttgccgg cggcctgctg cggcgagaac ggaatgatct gcacgaaagg ctcgatctgc
     9481 ttgagcacct tcgggtcgac gccgtaccag gccaggtcgc gcacttcgat gggcatcagg
     9541 atgctgttgg ggtcgacctg cgtgccgggg ttggcggccg cctgctgcgc gtcctggctc
     9601 gcgcgcatgc ccagcgcgat ggtgttggcg acgtccagcg agacgccgat ggcctggaag
     9661 agcagctgca gtcgctcgat ctcggtcttc ttgccaagct gctggccgtc cggtggcagg
     9721 gtgaggttgt agaggttgta cttggactgc gcgtcggtga tctggccgga aaggtaagcc
     9781 tccgggccgc tgtcctcggc gtggctgccg tcggccgaca ggaagctcga cagcttggtc
     9841 tcggccagcg gcgtggccca cggctggccg aggtcgtcct cggtgtggcc ctggcggatc
     9901 gacgatttca ggatcaggtt gccccagtcc agcgacgcgt tgagcagcca ctgggcctgg
     9961 gcgcgcgcgc gctcggccgt ctcgacctcg atgccgcgcc actgcagcca caccatgccg
    10021 gcggccaggg tggccaccag cgtcatgatc agcagcgcca gcagcagggc ggcgccacgc
    10081 tggcggcgga cggtcgggtg cggcgtcatg tcagcctccg aagcgcggca gctggcgctc
    10141 gcgcatgatc tcgccttcgg gcaccttcag cacgatcttg atgccgtccg gttggggcgg
    10201 ggccggcggt ggcgtgcacg gcggctgggt gcaggccggc gggttgttct gcgccgcccc
    10261 ctgcgtcgac tggatgttgt tccagctgca gcccggcgtg ttgacggctg ccgggttgca
    10321 cgcgtagacc tggaagtcgt cgacgttctc cagcaccgtg attgcgccgc cgctgatcgc
    10381 ggtccattgc tggctgcgca tccacgcgtc ggtgagctcg gacatgtgga cgaccggcgc
    10441 gctggcccag cgctgccagc ggtggtcctg cagcgtccac agcaccagct ggatgccctg
    10501 cggcgagcgg cgcgcgatgc gcagcgtcgc cccgtcgaac atgatggcct tgggcagcac
    10561 cttgctgtcg accaccatgg acatgtcgta ctcgaactgc gacatgccgg tgttgagctt
    10621 gagcgtgcgg tcggtggagg cgagcgccgc gtccttggcc cgcatcagcg cgtccatgcc
    10681 gcgcatcgcc agcgccgaca gcaccgccat gatgaccagc gccaccatga cctcgaccag
    10741 cgtgaaaccc gacgggcgcg agcggcggac catcagtgcc ccatcaccgt ggagatcgtg
    10801 acgagctggc gcccgtcggt gtcggcgacg gtggcgtcga cgcggcggaa gctcgggttg
    10861 ggcgtgccct gcacgaccag cgtgcccttg aagtcgcggc ccagctgcgt gcaggcgaac
    10921 ggcgtcttgc cgaccggcgg catctggtcg agcaggcgca tgtcggtgag ctggttgtcg
    10981 gcgcaccact ggccgaggac gacgtcgctc aggcgctgcg aggtgtcgac gaccgagctg
    11041 gccgcgcgcg agccggcggc cagcgcgatg gcgacgatcg cgagcgcgac catcacctcg
    11101 atcagcgtga agccggcggc gcgcgggcgc gggcgtgacg tcatggcgcg ggcgcgccgg
    11161 tggcgctgtc gatgatctgg aacggcgaca ggccgtcggt gccgacggcc aggtcgcggt
    11221 cgcccaggtg cagcacgacg cgctgcgccg gcagcagcgg ctcggggccg agcttgagct
    11281 gcggcgcgcc cacgatgctg gccttggtgt cggattccag ccaggcgtgc ggcagctcct
    11341 tggcggcctt gggcggcagc ccggtgaact cgaagccgcc ggcggccacg ggctcccagc
    11401 gcacgtcggt gccgatgatg cgcgactggg tgcgcgcggt ctcgagcagc gcggccaggc
    11461 gcgcgccctc ggtctcgagc ttgctctgcg agcggtcgcg cagcgacagc gagatcatcc
    11521 cgctggccac ggccacgatg agcaccacga ccatcagctc cagcagcgtg aagccggccg
    11581 tggaccgacg cggcctactc ccaggagccg atgtccgcgt cgtcgccttc accaccgggc
    11641 tgcccgtcgg cgccgaagct gaacacatcg atttctccgt gagtgcccgg gttggcgtac
    11701 tggtaggggt ggtgccaggg atcctccggc agcttgtcga ggtagctctt ccagttgccg
    11761 gggatcgagc cggtcgtcgg cttgctcacc agcgcctgca ggccctggtc cgtgctcggg
    11821 tagcggccgt tgtcgagctt gtagatcttg agcgcgttca tcagcgagtt gatgtccgag
    11881 tgcgccgccg tgatcttggc ctgggccacg cgcgacagca cgttgggcgc gatgagcgcc
    11941 gcgagcacgc cgatgatgac gatcacgacc atcagttcga gcagcgtgaa gccgcgcgag
    12001 tggcgtcgtg cgatgagggc ttggttcatg aaagatcccg atgggccggt tccaggaagt
    12061 cttgaatcat aatccgcgca tgagttcccg ctggactggc tttttcgtct gggccctggt
    12121 ggcggcttgc accgcgttct gggcaatgaa gatcttcgcc gccacgcggc cggtgccccc
    12181 gacggcgcag gctccgcagg tggccgccgc caatgggccg atggtgcgcc tgttcggggc
    12241 ggtcgtcgtg gcgccgccga cggtgaacgc gccgccgccg gagagctcgc gcttccagct
    12301 cgtgggcgtc attgcgccgc cggccggcac ggcgcagggc ggctacgccg tcgtctccgt
    12361 ggacaaccag cccgcgcgca cgtggcgcgt cggcgccaac atcgaaggca acacttcttt
    12421 gctttccgtg agcaagcgtg gcgccgagtt cggcccgccg ggcggcccca cctcgttctc
    12481 gctgcagttg cccgagcccg cggccgccga gaccggcacg ctgcagcccg ccgtctcgca
    12541 gcccgatccg gcgcagctgc agcaacagca gctgcagcag gcgcagccgc agatgcagcc
    12601 gcagcagcag atgcaacccc agatgcagca gccgaacgtc cagccctcga tgcgaggcgg
    12661 cttcggccgc gcggcctcgc tggcgggccg gcgcggcttc cagggtccgc agggcaacaa
    12721 cggcacgatg ccgccgaaga tgggcgtccc gcccgaccag cagacgcaga acgacagcgg
    12781 cggcgtgcag cagtaaggca cccaagaacg aagccggcgc gacccgccgg ctttttcatt
    12841 ggccgcgtca gacgcccagg gccagtgcga cttcgacgag atcggcgaag cggtgtgccc
    12901 cggcgcgcgg gtcggcgctc gcatgcgcgc catcgggccg gcagatccag cccgcgtgca
    12961 ggcccgcggc cagcgcgccg ccgacgtcgg cgtcgaggtc gtcgcccagg tgcagcacct
    13021 gggccggcgc cgcgccggcg gcctcgcacg cggcgcggaa gatcgacggg tccggcttgg
    13081 ccacgccgtg cagccgggcc gacaccgacc ccgcgaagaa gcgctcgaga ccgacgcggt
    13141 gcagctccgc gttgccg
//
LOCUS       DATCRO010000777        12288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_560696, whole genome shotgun sequence.
ACCESSION   DATCRO010000777 DATCRO010000000
VERSION     DATCRO010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560696"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VIN75_21175"
     CDS             <1..1674
                     /locus_tag="VIN75_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEY8976741.1"
                     /translation="GNHAIRKVTPDGVVTTLAGNGRPGWRDGPGAQAQFDGPMGVAID
                     TQGRVIVADAYNDRIRAIAPDGTVTTLAGGAAPGDVDGPGAQARFDTPCGVFVDARGR
                     IVVADTLNDALRAIDAAGNVTTIARAAIDAPAKWLLHRPASLAQAGDGALFVGTLAGI
                     VTRVETDGSAQALHGLEEHGLVRPTGLAMGHESRLLVADAAASRLHVLAPQAAASAPR
                     VDGAIGPAPDRPLPDAGGRWPLRPQLAWHEVVGTIGEDRGDGKGETRDHFHDGLDVRG
                     DVGQEVLAIAAGKVTNPVATFGFGKINEGLAIGSLAYVHMHVGRGAHGAALDPSRFVP
                     VRDEAGKLVRVRVRRGTRFEPGDVLGSINPMAHVHLWMGVSGYGLNPLRLGPRGFVDT
                     VAPSIGSIEVRDGRDARLVKKEAGRLLLPRGELSLVVDAWDQVDGNLPRRRLGLYSLG
                     YQWLRADGTPLPGLERPTTAIEFARLPGDELVKDFYADRSGITVQGSSETHFRYRVGR
                     AAGRPSAEPPFSTAALGPGDYVLRIVAKDFSGNEAKAGRDLAVRIVEAK"
     gene            1823..3325
                     /locus_tag="VIN75_21180"
     CDS             1823..3325
                     /locus_tag="VIN75_21180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY8976742.1"
                     /translation="MSSLKARLDKIPEGSAALFFIQMFSTLGFAVLQVSLVLYATKKL
                     HFSDDLALSLMSLFGAFNYGLHLFGGYLGGRFLSNRNLFVGGMALQVAGCGLIATGSA
                     AMLYWGLALFLTGSGLNVTCINMMLTQRFTPDDPRREGAFLWNYAGMNVGFFIGFTVA
                     GLFNEDGRWAPMFIFATLGNLVAIVLSWAWWDALKDRSTPLLQATPARFRQRFVAGIA
                     ILVGLVPVVWGLLQHPDSTTTLVEVIAALVYSSLVWLTIRHPDRRERRNMFAYLMLAV
                     GSLVFWSLYQMSSSGLTLFFDRNVDPMVGALKIQPQWVQNINTFVIAVGGPLLAWLFS
                     QLRERGWRIDVPRQFATALLLMGVGFLVLPLGIALGDPATGRSAFIWIFLSYVLQSLG
                     ELLISPVGYAMIGKLAPHRYQGVMMGAWMLLTGLASIFAGALSKSIPTAQDAAPQVTN
                     AYYAMLFGRLGWGTVAVGVVLVLLIPFLRRLIADKDTPEVDELAPAVQPH"
     gene            complement(3443..4534)
                     /locus_tag="VIN75_21185"
     CDS             complement(3443..4534)
                     /locus_tag="VIN75_21185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976743.1"
                     /translation="MPERPIAAVPARLAFADGLRGLAALWVLLFHVSTGHHVDALLGV
                     LPAAVDRVVFTWGHGGVAVFFVLSGCVLALTAERVALDAAGAGRFVLRRLLRLAPPYV
                     VALGVAVALGLAKGESFGAAQLVAHLLFVQTLADVPPVGIVFWTLGIEFQFYVAFALL
                     TWLADASGAERAARTRTISRRVVAALALAWPLGWLATPEQPAGILPFWGQFMAGVLAW
                     DAMRGARASQVIAIAYAGLLLACGIARDDLFTGIAGATALALLAAHACGGLHAWLSGR
                     AAQALGRVSYSLYLMHVPTITLAAALLHRLPSHRGIAGELADASLILAACLAVAGAFW
                     RAIERPSIRWSHRLLLSTPAAPLRGGVRS"
     gene            complement(4527..6317)
                     /locus_tag="VIN75_21190"
     CDS             complement(4527..6317)
                     /locus_tag="VIN75_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY8976744.1"
                     /translation="MAYDYSSDNKRLELPNPFRVENMFLFACAAITLAGGLTGLMWAR
                     AAIQSHTTKLGIAPLLVGVVLLAAGLSFAVIAARRLRFFFGRGRPRSLAPEVAIGATG
                     NSPRADFYKAMLRQGGLEYPEPQGALNGVLYHALPRLITAPVVVQELAQRHFFNALSF
                     LVTLLSFVLAWGLFGTEESRPLISILYFVFGAVVLLRPLVSGNQARLGTGALIALIVA
                     AIVGPVAANLLGSALPSVRYSVTGQAAFLLACALVAVVLILCALHAQLDASPQTERSC
                     EQRTLSMNGPPAALITELDRTLQNEWVEKIPNRRYTRVEPVTHGAAGAGVFAGELLEE
                     TQPMPMSGTAAVTLASALQAPRHSWLVMLDLYATLLTVFGVVCALVYVHGFDPSRVLV
                     DGWGNTATVGYAAVCLTVAAFCFQSAGAIWGRFNFESELVWIELQGTWQSSRIGTGNQ
                     LSSRLQTENDVVRVESMTLRIWRARVESVVFGKDGTRQVTAMYATSQQAARLASDLEA
                     FAARQSVFVAPRAAEDGQRIEALRATESLLGGEPPARADALAHAGADVPRVARAPAGR
                     FCTACGTKAGPDARFCSACGTALPAPDAGA"
     gene            complement(6351..6551)
                     /locus_tag="VIN75_21195"
     CDS             complement(6351..6551)
                     /locus_tag="VIN75_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976745.1"
                     /translation="MASPGKKSYPLRIDPALWAEIERLAAQELRSANAQVEFLLREAL
                     ARRGRKIKPAPPEGAAPPDKDS"
     gene            complement(6890..7831)
                     /locus_tag="VIN75_21200"
     CDS             complement(6890..7831)
                     /locus_tag="VIN75_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8976746.1"
                     /translation="MTSNLASRGASQPTRIEYSERRLTSMNGYAMFVLAVVLAVVALT
                     CFVRIAEDMAAETVLLAHVLPGVLCVLVTAAIGAGLYTLQPNEAAIMTLFGQYAGTDR
                     AEGLRWANPFLVKRRISLRARNLNSPALKVNDRRGNPVEIGAVIIWRVEDTAKARFEV
                     DDVDLYVKLQSEAALRHVASLYAYDEADSEDANEVTLRGGLDAVAAALIDELTARFAS
                     AGVVVSDARLTHLAYAPEIAQVMLRRQQAEAVISARHKIVQGAVAMVESALTALSERN
                     VVALDDERKAAMVSNLLVVLCGDNEAQPIINAGTLYG"
     gene            8005..10215
                     /locus_tag="VIN75_21205"
     CDS             8005..10215
                     /locus_tag="VIN75_21205"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8976747.1"
                     /translation="MNFTPQHRARAPKWIALAIAAAAITTLAACAGGGAKPGAVSGEV
                     VTPVAPGLRGYPFAASRTTTRDYFGTSISDDFGWLENAGDPYTRSWIAAENAYGRRYL
                     DAMPARAELRQRLKSLLESSTNAYSALAERGGLVFALKSTPPAQRPVLVALKSLDDLG
                     SERVVFDPNALAADGSVSIEFFRPSPDGRRVAIALSAGDASTAVVRVVDVLTGQVLPG
                     EVAHVLSAGAGGDVAWAAGGAGFFCTQSGPPGSPALVQVVFHRLGATGADRVELSSGL
                     PRLARVRLESARDGRHVLALVENGRGGDVALYMKPSDTNGEGAWRRLSAETDGVRDAQ
                     FGDDDALWLHAIGNAPRGKILRVPLSDARAVAWDRLAAVAMPVDGALQRFAVAGGTLY
                     VAEGLGSAMRLRAIDLKTRRGTTLVLPPGSGVAALARVGRSDVAAQLTNWAEPSVWTR
                     VAGGRVRRTALAMTSDASFNDCDIVREVATSRDGTPVTVDILRPHGTRLDGRNPLLLM
                     PAGSLGAGVSPDFDVTRRVWLDRGGVIALATLRGATDAGEAWRADGVRTRRQNGVDDL
                     LAAADMLVKRGYTQPALLAARARGDGATAVAAAMTQRPELFRAVSIAQGRYDLLSLEH
                     DATGQYDEPGYGSVRERAQFDALHALSPLHAVRDGAPYPAVLLSAGMRDGRVNPAQSR
                     KMTARLQQADPSGHAILLRTDVSSGQAAQSALSAAIEQAADEYGFLLHEVVAAQ"
     gene            complement(10417..12069)
                     /locus_tag="VIN75_21210"
     CDS             complement(10417..12069)
                     /locus_tag="VIN75_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976748.1"
                     /translation="MTTSSRQALLRLSTLGAALGAAALLCACVVEAPPRRYVAPAPVY
                     QQPAPAPAPSYPQPAPAYDQSQDQDDPVVSVYVDPPLEQPEPIAVQWAPPPMLVEVPP
                     MQPYPDAVWTGGYWAWEGRWVWAAGRWSPPPRPYYTWVQPYYEHRGDVVVFVPGFWCA
                     PERHFVPPPPGLSISVAFAAPGLRIVGRAPIGPQGVFVPPPPGSRRGIIVPAPVGTPP
                     AVVVSAPAVVNVGMRVRGNVDVNSHNTVNNVTNITNVTNVTIEAPAGSTANGQAFQNQ
                     VPARAHLAAQLAPAGRPAAPQPVSNQAVPVFHPGQAAVQLPPAQPVRGVGERGANAPG
                     GARPGQQGRPEFDQPPRQPAPVNGQQPAWQQGQQAGQPPLNQQPGWQQRPGYGQPAQG
                     QQQGQPVQPGQPQGQQPAWQQGQQRQPGQGYGQPQQGQPQYQGQPQPMPQGQQPGWQQ
                     RPGYGQPQPGQAQQPAPQGQPQQGGYPQDQRRRGDNPHGPQGAQGQAGSQPQGGQPAV
                     QGQPQGQQHPAQDNRNAQKEERRKTPHEREEDREKKNGERER"
BASE COUNT         1641 a         4386 c         4540 g         1721 t
ORIGIN      
        1 ggcaaccatg cgatccgcaa ggtcacgccc gacggcgtcg tgaccacgct cgccggcaac
       61 ggccgtcccg gctggcgcga cggcccgggc gcgcaggcgc agttcgacgg tccgatgggc
      121 gtggcgatcg acacccaggg ccgggtgatc gtcgccgacg cctacaacga ccgcatccgc
      181 gcgatcgcgc ccgacggcac ggtgacgacg ctggccggcg gcgcggcacc gggcgacgtc
      241 gacggccccg gcgcgcaggc gcgcttcgac acgccgtgcg gcgtgttcgt cgacgcacgc
      301 ggccgcatcg tggtggccga cacgctcaac gacgcgctgc gcgccatcga cgccgccggc
      361 aacgtgacga ccatcgcacg cgccgcgatc gacgcgccgg ccaagtggct gctgcaccgg
      421 ccggcctcgc tcgcgcaggc cggcgacggc gcgctgttcg tgggcacgct ggcgggcatc
      481 gtcacgcgcg tcgagaccga cggcagcgcg caggcgctcc acggcctgga ggagcacggc
      541 ctcgtgcgcc cgacgggcct ggcgatgggc catgagtcgc gcctgctggt ggccgacgcc
      601 gcggcgtcgc gcctgcacgt gctggcgccg caggccgcgg cctcggcgcc gcgcgtcgac
      661 ggcgcgatcg gccccgcgcc ggatcgcccg ctgcccgacg ccggtggccg ctggccgctg
      721 cggccgcagc tcgcatggca cgaggtggtg ggcacgatcg gcgaggatcg cggcgacggc
      781 aagggcgaga cgcgcgacca cttccacgac ggcctggacg tgcgcggcga cgtcggccag
      841 gaagtgctgg cgatcgccgc cggcaaggtg accaatcccg tcgcgacctt cggcttcggc
      901 aagatcaacg agggcctggc gatcggctcg ctcgcctacg tgcacatgca cgtggggcgc
      961 ggggcgcacg gcgcggcgct cgatccgtcg cgcttcgtgc cggtgcgcga cgaggccggc
     1021 aagctcgtgc gcgtgcgcgt gcggcgcggc acgcgcttcg agccgggcga cgtgctgggc
     1081 agcatcaatc cgatggcgca cgtgcacctg tggatgggcg tgtcgggcta cggcctgaat
     1141 ccgctgcggc tggggccgcg cggcttcgtc gacacggtcg cgcccagcat cggcagcatc
     1201 gaggtgcgcg acggccgcga tgcccgactc gtcaagaagg aggccgggcg cctgctgctg
     1261 ccgcgcggcg agctgtcgct ggtggtggac gcctgggacc aggtcgacgg caacctgccg
     1321 cgccgccggc tcgggctgta cagcctgggc taccagtggc tgcgtgccga cggcacgccg
     1381 ttgccggggc tcgagcgtcc gaccacggcg atcgagttcg cgcgcctgcc gggcgatgaa
     1441 ctcgtcaagg acttctatgc cgacaggagc ggcatcaccg tccagggcag cagcgagacg
     1501 catttccgct accgcgtcgg ccgcgccgcc ggccgcccga gcgccgagcc gccgttctcc
     1561 accgccgcgc tgggccccgg cgactacgtg ctgcgcatcg tcgcgaagga cttctcgggc
     1621 aacgaggcca aggccgggcg cgacctcgcg gtgcgcatcg tcgaggcgaa atagccgtcc
     1681 gcaacgcact ggaacgccaa tttctgccgg ttgtctaggg gattgacccc gcccgccagg
     1741 aaagctccct tcgctaaaat ttgaggcaaa ccgcgcggcc ccacggccgc gcggctcttt
     1801 tcaagcgtcg aaagcgattc ccatgtcttc cctcaaggcc cggctggaca agatccccga
     1861 gggctcggcc gccctgttct tcatccagat gttctcgacg ctgggtttcg ccgtgctgca
     1921 ggtgtcgctg gtgctgtacg cgaccaagaa gctgcacttc agcgacgacc tcgcgctgtc
     1981 gctgatgagc ctgttcggcg cattcaacta cggcctgcac ctgttcggcg gttacctcgg
     2041 cggccgcttc ctgagcaacc gcaacctgtt cgtcggcggc atggccctgc aggtcgccgg
     2101 ctgcggactg atcgccaccg gcagcgccgc gatgctgtac tggggcctgg cgctgttcct
     2161 caccggcagc ggcctgaacg tcacctgcat caacatgatg ctgacgcagc gcttcacgcc
     2221 ggacgacccg cgccgcgaag gcgcgttcct gtggaactac gcgggcatga acgtgggctt
     2281 cttcatcggc ttcacggtgg cgggcctgtt caacgaggac ggccgctggg cgccgatgtt
     2341 catcttcgcg acgctgggca acctggtggc catcgtgctg tcgtgggcct ggtgggatgc
     2401 gctcaaggat cgctcgacgc cgctgctgca ggccacgccg gcgcggttcc gccagcgctt
     2461 cgtcgcgggc atcgccatcc tggtgggcct ggtgcccgtc gtgtgggggc tgctgcagca
     2521 tccggactcc accacgacgc tcgtcgaggt gatcgccgcg ctggtgtact cgtcgctcgt
     2581 gtggctgacc atccgccatc ccgatcgccg cgagcgccgc aacatgttcg cctacctgat
     2641 gctggcggtc ggctcgctgg tgttctggtc gctgtaccag atgtcgtcca gcggcctgac
     2701 gctgttcttc gaccgcaacg tcgacccgat ggtcggagcg ctcaagatcc agccgcagtg
     2761 ggtgcagaac atcaacacgt tcgtgatcgc cgtcggcggt ccgctgctgg cctggctgtt
     2821 ctcgcagctg cgcgagcgcg gctggcgcat cgacgtgccc aggcagttcg cgaccgcgct
     2881 gctgctgatg ggcgtcggct tcctcgtgct gccgctgggc atcgcgctcg gcgaccccgc
     2941 gacgggccgc tccgcgttca tctggatctt cctgagctac gtgctgcaga gcctgggcga
     3001 gctgctgatc tcgccggtgg gctacgccat gatcggcaag ctcgcgccgc acaggtacca
     3061 gggcgtgatg atgggcgcat ggatgctgct gacgggcctg gcctcgatct tcgccggcgc
     3121 gctctccaag tcgatcccga ccgcgcagga cgccgcgccg caggtgacca acgcctacta
     3181 cgcgatgctg ttcggccggc tcggctgggg cacggtggcg gtcggcgtgg tcctggtgct
     3241 gctgatcccg ttcctgcgcc gcctgatcgc cgacaaggac acgcccgagg tcgacgagct
     3301 cgcgccggcg gtgcagccgc actgagggcg ccggcggcgc ggcgacgcac ccccacgcgt
     3361 cgaggcgcca ccgggcgatg tcaggtcttg aatcgtgcag tgcgagccat cggccgatgg
     3421 ccatgcgccc gtggtgcacg attcaagacc tgacaccgcc tcgaagcggc gcggcgggcg
     3481 tggacaggag cagccgatgg ctccagcgga tgctcgggcg ctcgatcgcg cgccagaagg
     3541 ccccggccac cgccaggcag gcggcgagga tgagcgaggc gtcggcgagc tcgccggcga
     3601 tgccccggtg cgaaggcagc cggtgcagca gcgcggcggc gagcgtgatg gtgggcacgt
     3661 gcatcaggta caggctgtac gagacgcggc ccagcgcctg cgcggcgcgg cccgacagcc
     3721 acgcgtgcag cccgccgcag gcgtgcgcgg cgagcagcgc cagcgccgtg gcgccggcga
     3781 tgcccgtgaa caggtcgtcc cgcgcgatgc cgcaagccag cagcaggccg gcgtacgcga
     3841 tcgcgatgac ctgcgacgcg cgcgctccgc gcatcgcgtc ccacgccagc acgccggcca
     3901 tgaactggcc ccagaagggc aggatgcccg cgggctgctc gggcgtcgcg agccagccca
     3961 gcggccacgc gagtgcgagc gcggcgacga cgcggcgcga gatcgtccgc gtgcgcgcgg
     4021 cccgctcggc gccggatgca tcggcgagcc aggtcagcag cgcgaacgcg acatagaact
     4081 ggaactcgat gccgagcgtc cagaacacga tgcccacggg cggcacgtcc gccagcgtct
     4141 gcacgaacag caggtgcgcg accagctgcg cggcaccgaa cgattcgccc ttcgcgaggc
     4201 cgagggcgac ggccaccccc agcgcgacga catagggcgg cgcgaggcgc agcaggcggc
     4261 gcagcacgaa gcggccggcg ccagccgcgt cgagcgcgac acgttcggcc gtcagtgcca
     4321 gcacgcagcc gctcagcacg aagaagacgg ccacgccgcc atggccccac gtgaagacga
     4381 cgcgatcgac cgcggccggc agcaccccga gcagcgcgtc gacgtgatgg cccgtcgaca
     4441 cgtggaacag cagcacccac agcgccgcga ggccgcgcaa gccgtcggcg aaggccagcc
     4501 gggccgggac ggccgcgatc ggacgctcag gcaccggcgt cgggcgcagg cagggcggtg
     4561 ccgcaggcgc tgcagaacct tgcgtcgggg ccggccttgg tgccgcaagc cgtgcagaag
     4621 cggcccgcgg gggcgcgcgc gacgcgcggc acgtccgcgc cggcgtgcgc gagcgcgtcc
     4681 gcgcgcgcgg gcggctcgcc gcccagcagc gactctgtgg cccgcagcgc ctcgatgcgc
     4741 tggccgtcct cggccgcgcg cggggcgacg aacacgctct ggcgcgcggc gaaggcctcg
     4801 aggtcgctgg ccaggcgcgc ggcctgctgc gacgtcgcgt acatcgccgt gacctggcgc
     4861 gtgccgtcct tgccgaacac caccgactcg acgcgcgccc gccagatgcg cagagtcatc
     4921 gactccacgc gcacgacgtc gttctcggtc tgcagccggc tgctcaactg gtttccggtg
     4981 ccgatgcgcg aggattgcca ggtgccctgc agctcgatcc acaccagctc cgactcgaag
     5041 ttgaagcggc cccagatcgc gcccgccgat tggaagcaga acgccgcgac cgtcaggcac
     5101 accgcggcgt agcccaccgt ggcggtgttg ccccagccgt cgaccagcac gcgcgacgga
     5161 tcgaacccgt gcacgtagac gagggcgcac acgacgccga acacggtcag cagcgtggcg
     5221 tacagatcga gcatcacgag ccagctgtgg cgcggcgcct gcagcgcgct ggccagcgtc
     5281 acggccgcgg tgcccgacat cggcatcggc tgcgtctcct ccagcagctc gccggcgaag
     5341 acgccggcgc cggccgcgcc gtgcgtcacg ggctcgacgc gcgtgtagcg ccggttcggg
     5401 atcttctcga cccactcgtt ctgaagcgtg cgatcgagct cggtgatcag cgccgcgggc
     5461 ggaccgttca tcgacagcgt gcgctgctcg cagctgcgct cggtctgcgg cgacgcgtcc
     5521 agctgcgcgt gcagcgcgca caggatcagc acgaccgcca ccagtgcgca cgccagcagg
     5581 aacgccgcct ggcccgtcac cgaatagcgt acggatggca gggccgagcc cagcaggttg
     5641 gccgccaccg ggccgacgat ggccgcgacg atcagcgcga tcagcgcgcc cgtgcccagc
     5701 cgcgcctggt tgccgctgac cagcggccgc agcagcacca ccgcgccgaa cacgaagtac
     5761 aggatcgaga tcagcgggcg cgattcctcg gtgccgaaca ggccccaggc gagcacgaag
     5821 ctcagcagcg tgacgaggaa ggacagcgcg ttgaagaagt ggcgctgcgc cagttcctgc
     5881 accacgaccg gcgcggtgat caggcggggc agcgcgtggt acagcacgcc gttgagcgcc
     5941 ccctgcggct cggggtactc gaggccgccc tggcgcagca tggccttgta gaagtcggca
     6001 cgcggcgagt tgccggtcgc gccgatcgcg acctcgggcg ccagcgagcg cggccggccg
     6061 cggccgaaga agaagcgcag ccgccgtgcc gcgatgaccg cgaacgacag ccccgccgcc
     6121 agcagcacca cgcccaccag cagcggcgcg atgcccagct tggtcgtgtg cgactggatc
     6181 gcggcgcgcg cccacatcag cccggtcagg ccgccggcca gcgtgatcgc ggcgcaggcg
     6241 aacaggaaca tgttctcgac ccggaacggg ttgggcagtt cgagccgctt gttgtccgag
     6301 ctgtagtcgt aggccaatcg tcgctcccgc gtcctgggtg tgcggcgatt ctaggagtcc
     6361 ttgtccggcg gggcggcgcc ttcgggcggc gccggcttga tcttgcgccc gcggcgcgcg
     6421 agcgcttcgc gcagcaggaa ctcgacctgc gcgttggcgc tgcgcagctc ctgcgcggcc
     6481 aggcgctcga tctcggccca cagcgcgggg tcgatgcgca gcggatagga cttcttgccg
     6541 gggctggcca tgggtcgtcg atgggtctca gtgatctcgg taggggtctg gcctctcctc
     6601 ggatcgcgcg ggaggcgcgg agcgaagacg gggcatcggc cgcgattcga ggcgaaggcc
     6661 tgaccccggg tcgctcacga tcggggtcag gccttcgcct gcatccgcga tcggcgtgat
     6721 cgctcgcggc gacatccagg cgcggaagca ggagaggcca gacccctccg gctccggcgc
     6781 agcgaaggag aggccggacc cctccggctt cggcgcagcc aaggagaggc caggcccctg
     6841 cgtctgccgt gacgcgtcca tgtccgattg cgtcgacgcg tgcatcgcgt cagccgtaca
     6901 gcgtgccggc gttgatgatg ggttgcgcct cgttgtcgcc gcacagcacc accagcaggt
     6961 tgctgaccat cgccgccttg cgctcgtcgt ccagggcgac gacgttgcgc tcgctgagcg
     7021 ccgtcagcgc cgactcgacc atcgccaccg cgccctgcac gatcttgtgg cgcgcgctga
     7081 tgacggcctc ggcctgctgg cggcgcagca tcacctgcgc gatctcgggg gcgtaggcca
     7141 ggtgcgtgag gcgggcgtcg gagacgacca cgccggcgct ggcgaagcgg gcggtcaact
     7201 cgtcgatcag cgccgccgcc acggcgtcca ggccgccgcg cagcgtgacc tcgttcgcgt
     7261 cctcgctgtc ggcctcgtcg tacgcgtaca gcgacgcgac atggcgcaac gcggcctccg
     7321 attgcagctt gacgtacagg tcgacgtcgt ccacctcgaa gcgcgccttg gcggtgtcct
     7381 cgacgcgcca gatgatgacc gcgccgatct cgaccggatt gccgcgcctg tcgttgacct
     7441 tgagcgccgg cgagttcagg ttgcgcgcgc gcagcgagat gcgacgcttg actaagaacg
     7501 ggttggccca gcgcaggccc tcggcgcggt cggtgccggc gtactggccg aacagcgtca
     7561 tgatggccgc ctcgttgggc tgcagcgtgt acaggccggc gccgatcgcc gccgtcacca
     7621 gcacgcacag cacgccgggc agcacgtggg cgagcagcac cgtctcggca gccatgtcct
     7681 cggcgatcct gacgaagcac gtcagcgcga cgacggccag caccacggcg agcacgaaca
     7741 tcgcatagcc gttcatcgac gtgaggcggc gctccgagta ttcgatgcgc gtcggctgcg
     7801 atgcgcctcg tgaggccagg ttggaggtca tggaaactcc cggtcaggta tcaaagtgat
     7861 atcactttag ggcgccgctg ccgggctgtc aatcgtgcgc ggcgtgcggt cgtgcgcacg
     7921 ttttgcaaca ccggcgggcg caaggcggcc ccgccgccgc ctaacatcgc gggcggagca
     7981 gattccgacg acgaggcaga ccgcttgaac ttcacgccgc agcaccgcgc ccgggcgccg
     8041 aaatggatcg cgctggccat cgccgccgcc gcgatcacca cgctggccgc ctgcgcgggc
     8101 ggcggcgcga agccgggggc ggtcagcggc gaggtcgtca cgcccgtcgc gccggggctg
     8161 cgcggctatc cgttcgcggc ctcgcgcacc acgacgcggg actacttcgg gacgtcgatc
     8221 agcgacgact tcggctggct cgagaacgcc ggcgatccgt acacgcgctc gtggatcgcg
     8281 gccgagaatg catacggccg gcggtatctg gacgcgatgc ccgcgcgggc cgaactgcgc
     8341 cagcggctga agtcgctgct cgagtcgagc acgaatgcgt actcggcact cgccgagcgc
     8401 ggcggcctcg tgttcgcgct gaagtcgacg ccgccggcgc aacgtccggt gctggtcgcg
     8461 ctgaagtcgc tcgacgacct cggcagcgag cgcgtcgtgt tcgacccgaa cgcgctggcg
     8521 gccgacggct cggtctcgat cgagttcttc cggccgtcgc ccgacggccg ccgcgtcgcg
     8581 atcgccttgt cggccggcga cgcgagcacc gccgtcgtgc gcgtggtgga cgtcctcacc
     8641 gggcaggtgc tgccgggcga ggtggcgcac gtgctctcgg ccggcgcggg tggcgacgtc
     8701 gcgtgggcag cgggtggcgc cggcttcttc tgcacgcagt ccgggccgcc gggctcgccg
     8761 gcgctcgtgc aggtggtgtt ccaccggctc ggcgcgacgg gcgccgatcg cgtcgagctg
     8821 tcaagcggcc tgccgcgcct cgcgcgcgtg cgcctcgaga gcgcgcgcga cggcaggcac
     8881 gtgctcgcgc tggtggagaa cgggcgcggc ggcgacgtcg cgctgtacat gaagccgtcc
     8941 gacacgaacg gcgagggtgc ctggcgccgc ctctccgccg agaccgacgg cgtgcgcgac
     9001 gcccagttcg gcgacgacga cgcgctgtgg ttgcatgcga tcggcaacgc gccgcgcggc
     9061 aagatcctgc gcgtgccgct gtctgacgcc agggcggtcg cctgggaccg gctcgccgcc
     9121 gtcgccatgc cggtcgacgg cgcgctgcag cgcttcgcgg tcgccggagg caccctctac
     9181 gtggccgagg gactgggcag cgcgatgcgc ctgcgcgcga tcgacctgaa gacccggcgc
     9241 ggcacgacgc tcgtgctgcc gcccggaagc ggcgtggccg cgctcgcgcg cgtggggcgc
     9301 agcgacgtcg ccgcgcagct caccaactgg gccgagccct cggtgtggac gcgcgtggcc
     9361 gggggacgcg tgcgccgcac cgcgctcgcg atgacctcgg acgccagctt caacgactgc
     9421 gacatcgtgc gcgaggtcgc gacgtcgcgc gacggcacgc cggtgaccgt cgacatcctg
     9481 cgcccgcatg gcacgcggct cgacggccgc aacccgttgc tgctgatgcc ggccggctcg
     9541 ctcggggcgg gcgtgtcgcc ggacttcgac gtgacgcgcc gcgtgtggct cgatcgcggc
     9601 ggcgtcatcg cgctggccac gctgcgcggc gcgaccgacg cgggcgaggc ctggcgcgcc
     9661 gacggcgtgc gcaccaggcg ccagaacggc gtggacgacc tgctcgccgc ggccgacatg
     9721 ctggtcaagc gaggctacac ccagcccgcg ctgctggccg cccgcgcgcg cggcgacggc
     9781 gcgactgccg tcgcggccgc gatgacgcaa cgtccggagc tgttccgcgc agtgtccatc
     9841 gcgcaaggcc gctacgacct gctttccctc gagcacgacg ccaccggcca gtacgacgag
     9901 cccggctacg gcagcgtccg cgagcgcgcg cagttcgacg cgctgcacgc gctctcgccg
     9961 ctgcacgcgg tgcgcgacgg cgcgccgtat cccgcggtgc tgctgtcggc cggcatgcgc
    10021 gacggccgcg tcaatccggc gcagtcgcgc aagatgaccg cacgcctgca acaggccgat
    10081 ccgagcggac acgcgatcct gctgcgaacc gatgtctcca gcggacaagc tgcgcagtcg
    10141 gccctgtcgg ccgccatcga acaggcggcc gatgaatacg ggttcctgtt gcatgaggtg
    10201 gtcgcggccc agtgaggggt ggggctcgca tgccgatccc ctggcgatcg gcgtcaggca
    10261 cggccgcgaa cgcgatccgc gatgcggagg acggccggcg cgtcggcgcg attcgagaag
    10321 gtgcgagaca ccttcggcgg cggctcggtg cgcccatgcg aaaaaaaagg aggcaccgcg
    10381 gtgcctcctt ccctttgcgc ctcgcgacgt cgaggatcaa cgctcgcgtt cgccgttctt
    10441 cttctcgcgg tcttcctcgc gttcgtgcgg cgtcttcctg cgctcttcct tctgcgcgtt
    10501 gcgattgtcc tgcgccgggt gctgctggcc ttgcggctgg ccctggacgg cgggttggcc
    10561 gccttgcggc tggctgccgg cctggccttg cgcgccttgc ggaccgtgcg ggttgtcgcc
    10621 gcgccggcgc tggtcctgcg ggtagcctcc ttgttgcggc tggccctgcg gcgccggttg
    10681 ctgggcctgg ccgggctgcg gctggccgta gccggggcgc tgttgccatc cgggttgctg
    10741 gccttggggc atcggctgcg gctggccctg gtattgcggc tgcccttgct gcggctggcc
    10801 atagccctgg cccggctggc gctgctggcc ttgctgccac gcgggctgct ggccctgcgg
    10861 ctggcccggt tgcaccggct ggccttgttg ctggccttgc gccggctgtc cgtagcccgg
    10921 gcgttgctgc caacccggct gttggttcag cggcggctgg ccggcctgct ggccttgctg
    10981 ccacgccggc tgctgcccat tgacaggggc gggctggcgc ggcggctggt cgaactcggg
    11041 gcggccctgc tggccggggc gggcgccgcc gggcgcgttg gcaccgcgtt caccgacgcc
    11101 gcgcaccggc tgggccggcg gcaactgcac cgcggcctgg cccgggtgga acaccggcac
    11161 ggcctggttg gacaccggct gtggcgccgc ggggcggccg gcgggcgcga gctgcgccgc
    11221 caggtgcgcg cgggcgggca cctggttctg gaacgcctgg ccgttggccg tgctgccggc
    11281 cggggcctcg atcgtcacat tggtcacgtt cgtgatattg gtgacgttgt tgacggtgtt
    11341 gtggctgttg acgtcgacgt tgccgcgcac gcgcatgccc acgttgacga cggccggcgc
    11401 gctcacgacg accgcaggcg gcgtgcccac cggcgcgggc acgatgatgc cgcggcgcga
    11461 gcccggcggc ggcggcacga acacgccttg cgggccgatc ggggcgcgcc cgacgatgcg
    11521 caggcccggc gcggcgaacg ccaccgagat gctcagcccc ggcggcggcg gcacgaagtg
    11581 gcgttccggc gcgcaccaga agcccggcac gaacacgacg acgtcgccgc ggtgctcgta
    11641 gtacggctgc acccacgtgt agtacgggcg cggcggcggc gaccagcggc ccgcggccca
    11701 gacccagcgg ccttcccacg cccagtagcc gcccgtccac acggcgtcgg ggtagggctg
    11761 catcggcggc acttcgacca gcatcggcgg cggcgcccat tgcacggcga tcggctcggg
    11821 ctgctccagc ggcggatcga cgtagacgct gacgaccggg tcgtcctggt cctgcgactg
    11881 gtcgtaggcc ggcgcgggtt gcggatagct cggggcgggc gcgggggccg gttgctggta
    11941 cacgggcgcc ggcgcgacgt agcgccgcgg gggcgcctcg acgacgcacg cgcaaagaag
    12001 ggcggcggca ccgagcgcgg cgccgagcgt gctcaggcgc agcagggcct ggcgggacga
    12061 tgtggtcatg tcgggagagt cctctggtgt cgccccggcc gtgcgggcgg attcgggcat
    12121 tgtcgcgggc ccgtgcgggt tcaacgtccg agtggcggag ttggtgggca ggcgacacgc
    12181 aaatttacat tccggggcgc ctgcgcccgg cccgcgcgca tgcgggcaag cggtcgatcg
    12241 actgtcgccg cgcggtccgc ctgtcgttcg tgtggcgcgc ggcggccg
//
LOCUS       DATCRO010000778         8683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_908678, whole genome shotgun sequence.
ACCESSION   DATCRO010000778 DATCRO010000000
VERSION     DATCRO010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908678"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..2382
                     /locus_tag="VIN75_21215"
     CDS             493..2382
                     /locus_tag="VIN75_21215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY8976749.1"
                     /translation="MSGPAPAPIPTPAGSAAGTGPAAATREQAPPPAGVTRGTRASRQ
                     WSLASPWLMGAVLLVAAWLAWFTVDVLQERLHESSAKFDAGVHRFERFWNLHGAALEA
                     TLAVKWDLAAGDAAADGLEVDAARLRERSAALHDELSQPGATLSQTAAWTAAVARLSD
                     DLARRRELADPAGALALTQAVLDDLARVGDEARQRQAMSAAFLAEVQAVRHQVLLAFA
                     LIAAMLVLVVALAAQWMLVARRERAIRGRAEHLAAVAESERARAEAADREKGRFLGML
                     SHELLTPLQSIWSTIDVIESRGRIESTDASFRRLKESTRSLRGRISDLLDFAKMSSGR
                     LETRIRGFQLDKLIDSALRDVEEALAERNLDVHWEAGPELGQRIYSDPARLRQVIDNL
                     LTNAIKYTERGGITIVAELRPDENLLRVEISDTGAGIAPEAMARLFEPFYRSPLTAGM
                     AEGSGLGLAVVRSLVELMGGTIRFESQPGEGTVATVEVPITEGTDGLVVDAPPRLDTR
                     LPVLVVDDSRDARRAIAEVIRSLGIDVVEAADGTTGLAAAAAGEFQAILLDLQMPDLT
                     GLQVAQELRRPGNRHGKTFLALVSAYNDLDDKTLDAVFDARVDKPVGRKELAAVLGRA
                     AQARR"
     gene            complement(2811..3401)
                     /locus_tag="VIN75_21220"
     CDS             complement(2811..3401)
                     /locus_tag="VIN75_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976750.1"
                     /translation="MALPAAPDTLLSDTPHAALPCVLDIEASGFGRGSYPIEIGFVLP
                     DGSAYCSLIVPDSDWTHWDGDAERVHGISRAVLQRHGRSVHEVAHELNQRLDGRTVYC
                     DNWAHDYAWLARLFESADVVPTFRLRHLRELMSENAAERFDAAREVVERNLQLRRHRA
                     SSDARVLQLSVARVWRNGGGVGEGAGMSGTARGMPS"
     gene            3619..5388
                     /locus_tag="VIN75_21225"
     CDS             3619..5388
                     /locus_tag="VIN75_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8976751.1"
                     /translation="MSSSEAYSRTSPIDESLVDTNDGEVPADRPHFASWPRRLPRTVT
                     PPETSLWHNVEVASQRYPAKAATLFFGRAKSFAGLRADAEAIAGWLQAQGVRRGDRVA
                     LFMQNCPQYLVAYHAILRADAVVVPVNAMNKVEEFGHYIVDPGTKVVICGAELAAIVA
                     GANAALPPSQRATCVLATRYADELPEAFDEDVAPSAAMRDWLLADPPLPPGTVRWVDA
                     LAERHVPGPTVNGPDDLALLPYTSGTTGLPKGCMHTHRTLMANVVGGAEWGSGSAESI
                     VLGVVPMFHITGMLYGVISPMFGGATVVIMPRWDRELAGRLISRHKVSHWTCIPTMII
                     DLFASPNYRSFDLSSLRWLSGGGAAMPQAVAQRLIDEFGLHFCEGYGLTETAAPSHAN
                     PAERPKLQCLGMPFFGVDSRVVDPVTLKELPPGEVGEIVTRGPMVFRGYWKNPEATAA
                     AFVEVEGRKYFRTGDLGHMDTDGYFFITDRLKRMINASGFKVWPSEVELLLYKHPGVQ
                     EACVISAQDAYRGETVKAIVVLRADAKGKVQERDIVSWAHENMAAYKAPRIVEFVDAL
                     PKSGAGKVMWRLLQDKENARGAG"
     gene            complement(5606..6502)
                     /locus_tag="VIN75_21230"
     CDS             complement(5606..6502)
                     /locus_tag="VIN75_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8976752.1"
                     /translation="MNVTFRQLRVFQSVARTRNFSRAGEAVGLTQPAVSRAIGELEHA
                     MGLRLLDRTTREVALTEAGRSLAARLDRVLDELDATLDDVAGLADADGGKVRVASSPT
                     LSAYLMPDCIAACARAAPRVRFLLLDRIQQDVLASVRGGEVDFGVVVEPPEVDDLHCE
                     DILRDPFVLVTPEQHRFARGASVRWKALDGASLVLLDSASGSRRYVDEALAQHGVACK
                     VEQELGHPTTAFQMVEAGIGLTVMPRLAVPPAGLPGLAVRPLLPRLERTVMLVRRHDR
                     ALSPVAERVWGLVSATVRGRGR"
     gene            6572..7576
                     /locus_tag="VIN75_21235"
     CDS             6572..7576
                     /locus_tag="VIN75_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY8976753.1"
                     /translation="MKRPSFVPDNFTLALVGVVALASVLPAQGVAARLFDVATGGVIG
                     LLFFLHGAKLSREAIKSGLMHWRLHVLVFACTFGLFPLLGLALKPLLQPLVTRELYTG
                     VMFLCVLPATVQSAIAFTSIARGNMPAAVCSASASTLLGVFVTPVLVDLVVVPNAADA
                     HALDSIGRILMQLMLPFAAGHLARPLIAGWMHRRKEVVGLVDRGSILLVVYTAFSAAV
                     IEGLWKQVPPLALAGLLVVCAVLLACALFLTGWFARRLGFDKADEITIVFCGSKKSMA
                     SGIPMAKVLFPAKSVGAIVLPLMLFHQMQLMVCAVLAQRWRRRVEHDAVGGTPSGAVG
                     "
     gene            7630..8271
                     /locus_tag="VIN75_21240"
     CDS             7630..8271
                     /locus_tag="VIN75_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976754.1"
                     /translation="MGMHFGIVAVKAGAAQFVDAFATAWPHHEPAARVDVTGLEALSD
                     WMRATQRNAAAAGGAVDVFGFWQDGMWAVLFDPSYAQASDRRALAVLSQRFGLVLSFV
                     VETAGGCAFFEAFDGGQLVRRIQSIDGVLTTDGVRLPQEAGLPADRYFEDETRQLQRA
                     FGLTGMDGVPADVPVIGAAYVDRTDYGTPRRRPSAAADRVATPSLKRPWWRVW"
     gene            complement(8424..>8683)
                     /locus_tag="VIN75_21245"
     CDS             complement(8424..>8683)
                     /locus_tag="VIN75_21245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976755.1"
                     /translation="HNEARQRFEATVEGQLCVCDYQLRGNVMWMTHTGVPPAVGGRGI
                     AAELVRVALAWADAKEYRVQPSCSYVDVYMRRHPETQKLRA"
BASE COUNT         1195 a         3025 c         3142 g         1321 t
ORIGIN      
        1 gcccgcctac ccgctggcgc cagcgtacgc gagctggctg ggcgaggggc ccgtcgggcg
       61 gtgcgaggcc gtcggcgatg acgatgtccg gtgcgttgtc ggtgtccact gggctctccg
      121 ttgcaggcct ccacggtatg ccagggccgc ccgggacgcc cgcggcaaac aggcgggttt
      181 cggccgcatc cggcgcgcgg cagggcaatc gcgaaggggc gcgaagatcg caggcgagga
      241 ctgcaatggc cgtggcgcgc caaatgccgc atttcgggcc cggatgtcac gggcggcgct
      301 ttcgagctga catccagcct tcgcgcagcg cccggaacgg caccccgctc gtatcatgaa
      361 gacgcgctac cgatccggcc tcgctttcgc gtcgccgcct gcgccggagt ccttcccctg
      421 tccaccaagc ccgtcaccgt cgccccgccc ctcggcaccg cgcccgcgtc gctggcgacg
      481 cgcgcggccg ccatgagcgg gcctgccccg gcgccgatac cgacgccggc aggctcggcg
      541 gccggcacgg gcccggctgc ggccacgcga gagcaggcgc ccccgcccgc gggcgtcacg
      601 cgcggcacgc gcgccagccg ccagtggtcg ctcgcgtcgc cgtggctgat gggcgcggtc
      661 ctgttggtgg cggcctggct cgcctggttc acggtcgacg tgctgcagga gcggctgcac
      721 gagtccagcg cgaagttcga tgccggcgtg caccgcttcg agcgcttctg gaacctgcac
      781 ggcgccgcgc tggaggccac gctggcggtc aagtgggatc tggccgcggg cgacgccgcc
      841 gccgacggcc tggaggtcga tgcggcccgc ttgcgcgagc gctcggccgc gctgcacgac
      901 gagctttcgc agccgggcgc cacgctgtcg cagacggccg cgtggacggc cgcggtcgcc
      961 cggctgtccg acgacctcgc gcgccggcgc gagctggccg acccggccgg tgcgctggcg
     1021 cttacccagg ccgtgctgga cgacctcgcc cgggtcggcg acgaggcgcg ccagcgccag
     1081 gcgatgtcgg cggccttcct ggccgaggtg caggcggtgc ggcaccaggt gctgctcgca
     1141 ttcgcgttga tcgcggcgat gctggtgctg gtcgtggcgc tggccgcgca gtggatgctc
     1201 gtggcgcggc gcgagcgcgc catccgcggt cgcgcggagc acctggccgc ggtggccgaa
     1261 tcggagcggg cccgcgccga ggcggccgat cgcgagaagg gccgcttcct gggcatgctg
     1321 agccacgagc tgctcacgcc gctgcagtcc atctggagca cgatcgacgt catcgagtcg
     1381 cgcgggcgca tcgagtcgac cgatgcgtcg ttccgccggc tcaaggagag cacgcgctcg
     1441 ctgcgtggcc gcatcagcga cctgctggac ttcgccaaga tgtcgtccgg ccgcctcgag
     1501 acgcgcatcc gcggattcca gctcgacaag ctgatcgaca gcgcgctgcg cgacgtcgag
     1561 gaggcgctgg ccgagcgcaa cctcgacgtg cactgggagg ccggtcccga gctgggccag
     1621 cgcatctact ctgaccccgc gcgcctgcgc caggtgatcg acaacctgct caccaatgcc
     1681 atcaagtaca cggagcgcgg cggcatcacc atcgtggcgg agctgcggcc cgacgagaac
     1741 ctgctgcgcg tcgagatctc cgatacgggc gccggcatcg cccccgaggc gatggcccgg
     1801 ctgttcgagc cgttctaccg ctcgccgttg acggccggca tggccgaggg cagcggcctc
     1861 ggactcgccg tggtgcgcag cctcgtcgag ctgatgggtg gcaccatccg cttcgagtcg
     1921 cagccgggcg agggcaccgt ggccaccgtg gaggtgccca tcaccgaagg caccgacggc
     1981 ctggtggtcg acgcaccgcc gcggctggac acgcgactgc cggtgctcgt ggtggacgac
     2041 tcgcgcgacg cgcggcgagc catcgccgag gtcatccgct cgctgggcat cgacgtggtc
     2101 gaggccgcgg acggcacgac cggcctggca gcggccgccg cgggcgagtt ccaggccatc
     2161 ctgctggacc tgcagatgcc cgacctcacc ggcctgcagg tcgcccagga attgcggcga
     2221 ccgggcaatc ggcacggcaa gaccttcctc gcgctcgtgt cggcctacaa cgacctggac
     2281 gacaagacgc tggacgccgt gttcgacgcg cgcgtcgaca agccggtcgg acgcaaggag
     2341 ctcgccgccg tgctcgggcg cgcggcgcag gcgcggcgat gaggtcgttg ctgtcggact
     2401 tggcgggtta cgccttcggc tgacccgccc taccctgacg tcgtggggcg cctgggcggg
     2461 tgctcgtgca tgactcgtga cgccgaggag cgacgatctg gaaacgtccc cggccgatcc
     2521 cgcattgcat cgcgcggtac cgtgcgggcg ggttggccgc aggcgtcacc tgccttccgt
     2581 cgaaaagtgc accagacgga ccgcaaggcg taaatgtcgc cggctagttt cgtgagttgg
     2641 aaattcgttg aacaggtctg gagcgaagat gtgacggtgc ccaattccgt ccagatcggt
     2701 tgcagaccag gcgcgcccgc gacacgatgc cgggtggcct cgcgagcggg cgcaacgacg
     2761 gacgcggccc ttctgggcgg aatgcttcaa cgaatttccg actcacgaca ctagctcggc
     2821 atgccgcggg ccgtcccgct catgcccgcg ccttctccga cgccgccccc gttgcgccac
     2881 acgcgcgcca cgctcaactg cagcacccgc gcatcactgc tcgcgcgatg ccggcgcagt
     2941 tgcaggttgc gttcgacgac ctcgcgggcc gcgtcgaatc gctcggcggc gttctcgctc
     3001 atcagctcgc gcaggtggcg caggcggaac gtggggacga cgtcggccga ttcgaacagg
     3061 cgggcgagcc aggcgtagtc gtgcgcccag ttgtcacagt acacggtacg gccgtccagc
     3121 cgttgattga gctcgtgggc gacctcgtgg acgctgcggc cgtggcgctg cagcaccgcg
     3181 cgcgagatgc cgtgcacgcg ttcggcgtcg ccgtcccagt gcgtccagtc cgagtcgggc
     3241 acgatcagcg agcagtacgc gctgccatcg ggcagcacga agccgatctc gatcgggtag
     3301 ctgccgcggc cgaagccgga cgcctcgatg tcgagcacgc acggcagcgc cgcatgcggc
     3361 gtgtcgctga gcagggtgtc gggtgcggcg gggagggcca tggggatggg gcgtgggtcg
     3421 cgctctcgcg agatacgctc ctctggcaat aatcggacga tacccgatcg agttgagcct
     3481 gtctcctgcg tgacagggcg gctaatggct cgcactatgg tgcctgcgcg gcgtgccaag
     3541 taggctgggg gtggccgcgc gacgtcgctg cggtgcagca atggagggcg ggctccgagg
     3601 tccgcaagaa ggaaatcgat gtcttcatcc gaagcgtatt cccgcacgtc cccgatcgac
     3661 gagtcgctgg tcgacacaaa tgacggcgag gtgcccgccg atcgtccgca tttcgcgtcg
     3721 tggccgcgcc ggctcccgcg caccgtgacg ccgcccgaga cctcgctgtg gcacaacgtc
     3781 gaggtcgcgt cgcagcgcta tccggccaag gccgcgacgc tgttcttcgg ccgcgccaag
     3841 tccttcgccg ggctgcgcgc ggatgccgag gcgatcgccg gctggctgca ggcgcagggc
     3901 gtgcggcgcg gcgaccgtgt cgcgctgttc atgcagaact gtccgcaata cctggtcgcg
     3961 tatcacgcga tcctgcgcgc ggatgccgtc gtcgtgccgg tcaacgcgat gaacaaggtc
     4021 gaggagttcg gccactacat cgtcgacccc ggcaccaagg tcgtgatctg cggcgccgag
     4081 ctggccgcga tcgtggccgg cgcgaacgcg gcgctgccgc cgtcgcagcg cgccacgtgc
     4141 gtgctcgcca cgcgctacgc cgacgagctg cccgaggcct tcgacgaaga cgtcgcgccg
     4201 tccgccgcga tgcgcgactg gctgctggcc gatccgccgc tgccgccggg caccgtgcgc
     4261 tgggtcgatg cgctggccga gcgccatgtg cccggcccga cggtcaacgg cccggacgac
     4321 ctcgcgctgc tgccctacac ctcgggcacc accggcctgc ccaagggctg catgcacacg
     4381 cacaggacgc tgatggccaa cgtggtgggc ggcgcggagt ggggctcggg cagcgccgaa
     4441 agcatcgtgc tgggcgtggt gccgatgttc cacatcaccg gcatgctgta cggcgtgatc
     4501 tcgccgatgt tcggcggcgc caccgtggtg atcatgccgc gctgggatcg cgagctggcc
     4561 ggccgcctga tctcgcgcca caaggtgtcg cactggacgt gcatccccac gatgatcatc
     4621 gacctgttcg ccagccccaa ctaccgcagc ttcgacctgt cgagcctgcg ctggctgtcg
     4681 ggcggcggcg cggcgatgcc gcaggcggtg gcgcagcgcc tgatcgacga gttcggcctg
     4741 cacttctgcg aaggctacgg cctgaccgag acggccgcgc ccagccacgc gaatccggcc
     4801 gagcgcccca agctgcaatg cctgggcatg ccgttcttcg gcgtcgactc gcgcgtggtc
     4861 gacccggtca cgctcaagga acttccgccc ggcgaggtcg gcgagatcgt cacccgcggc
     4921 ccgatggtgt tccgcggcta ctggaagaac ccggaggcca ccgcggcggc cttcgtcgag
     4981 gtcgagggcc gcaagtactt ccgcaccggc gacctgggcc acatggacac cgacggctac
     5041 ttcttcatca ccgatcgcct caagcgcatg atcaacgcga gcggcttcaa ggtgtggccc
     5101 tcggaggtgg aactgctgct gtacaagcac ccgggcgtgc aggaggcctg cgtcatctcg
     5161 gcgcaggacg cctaccgcgg cgagacggtc aaggccatcg tcgtgctgcg cgccgatgcc
     5221 aagggcaagg tgcaggagcg cgacatcgtc agctgggccc acgagaacat ggccgcgtac
     5281 aaggcgccgc gcatcgtcga gttcgtcgac gcgctgccca agtccggcgc gggcaaggtg
     5341 atgtggcggc tgctgcagga caaggagaac gcccgcggcg cgggctgacg gacggcgcgc
     5401 gtcatggcgt catgacgagg gcgtaccctc cgtgtctttc agcattcggg agcagacatg
     5461 agcaacacct tcgtctgggt cgacatcccc gtcaccgacc tcgaccgtgc ggtcatcctc
     5521 gacagcgagg gcaaccgcgt cgcgctgcat tcgatgaccg aatgatccgc cgtcgccgcg
     5581 cgggtgcgcc cgcgcgtgac gcgcgtcagc gcccgcgccc gcgcacggtc gcgctcacga
     5641 ggccccacac gcgctcggcc accggcgaca gcgcgcggtc gtggcggcgc accagcatca
     5701 cggtgcgttc cagccgcggc agcagcgggc gcacggccag gcccggcagg cccgcgggcg
     5761 gcacggccag gcgcggcatg acggtcaggc cgatgccggc ctcgaccatc tggaacgccg
     5821 tcgtgggatg tcccagctcc tgctccacct tgcatgcgac gccgtgctgc gcgagcgcct
     5881 cgtcgacgta gcgccggctg cccgacgcgc tgtccagcag caccagcgac gccccgtcca
     5941 gcgccttcca gcgcacgctg gcgccgcgcg cgaagcggtg ctgctcgggc gtgacgagca
     6001 cgaacgggtc gcgcaggatg tcctcgcagt gcaggtcgtc cacctccggc ggctcgacga
     6061 cgacgccgaa gtccacttcg ccgccgcgca cgctggctag cacgtcctgc tggatgcgat
     6121 cgagcagcag gaagcgcacg cgcggcgcgg cgcgggcgca ggcggcgatg cagtcgggca
     6181 tcaggtaggc cgacagcgtg ggactgctgg ccacgcgcac cttgccgccg tcggcatcgg
     6241 ccaggccggc gacgtcgtcc agcgtcgcgt cgagctcgtc gagcacgcgg tcgaggcgcg
     6301 cggccagcga gcggccggcc tcggtgaggg ccacctcgcg ggtggtgcga tccagcaggc
     6361 gcaggcccat cgcgtgttcc agctcgccga tggcgcggct gaccgccggc tgcgtcaggc
     6421 cgacggcctc gccggcgcgg ctgaaattgc gcgtgcgggc cacggactgg aacacgcgca
     6481 gctggcgaaa tgtcacattc atgatttcga atcataagtc catggaacaa atccatttct
     6541 cttttgagtg gcggcgcggt gaaatggcgc catgaaacgc ccgtccttcg ttcccgacaa
     6601 cttcacgctg gcgctggtgg gcgtcgtggc gctggcctcg gtcctgccgg cccagggcgt
     6661 cgccgcccgc ttgttcgacg tcgccaccgg cggcgtcatc gggctgctgt tcttcctgca
     6721 cggggccaag ctctcgcgcg aggcgatcaa gtccggcctg atgcactggc ggctgcacgt
     6781 gctggtgttc gcctgcacgt tcggcctgtt cccgctgctg gggctggcgc tcaagccgct
     6841 gctgcagccg ctggtcacgc gcgagctcta caccggcgtg atgttcctgt gcgtgctgcc
     6901 ggcgacggtg cagtcggcga tcgcgttcac gtcgatcgcg cgcggcaaca tgcctgcggc
     6961 ggtctgcagc gcgtcggcgt ccaccttgct gggcgtgttc gtgacgccgg tgctggtgga
     7021 cctggtcgtc gtgccgaacg ccgccgacgc ccacgcgctc gattcgatcg ggcgcatcct
     7081 gatgcagctg atgctgccgt tcgcggccgg ccacctcgcg cggcccctga tcgccggctg
     7141 gatgcatcgg cgcaaggagg tcgtgggcct ggttgaccgc ggctccatcc tgctggtggt
     7201 ctacaccgcg ttcagcgcgg ccgtcatcga gggcctgtgg aagcaggtgc cgccgctggc
     7261 gctcgcgggg ctgctggtcg tctgtgcggt actgctggcc tgcgcgctgt tcctcacggg
     7321 ctggttcgcg cgccgcctgg gcttcgacaa ggccgacgag atcacgatcg tgttctgcgg
     7381 ctccaagaag agcatggcca gcggcatccc gatggccaag gtgctgttcc ccgcgaagtc
     7441 ggtcggcgcg atcgtgctgc cgctgatgct gttccaccag atgcagctga tggtgtgcgc
     7501 cgtgctggcg cagcgctggc ggcggcgcgt cgagcacgac gccgtcggcg gcacgcccag
     7561 cggcgcggtg ggctgacccc tgcgtgctag ggtcgggtcc ggaccatcgg aatcgacagg
     7621 atcgccagcg tgggaatgca tttcgggatc gtcgccgtga aggcgggggc ggcgcagttc
     7681 gtcgacgcgt tcgcgacggc gtggccccac catgagccgg ccgcgcgcgt ggacgtgacc
     7741 ggcctcgaag ccctgtccga ctggatgcgc gccactcagc ggaacgccgc cgcggcaggc
     7801 ggggcggtcg acgtgttcgg cttctggcag gacggcatgt gggccgtgct gttcgacccc
     7861 agttacgcgc aggcctcgga ccgccgcgcg ctggccgtgc tcagccagcg cttcggactc
     7921 gtgctttcct tcgtggtgga aaccgcgggc ggctgcgcgt tcttcgaggc cttcgatggt
     7981 ggacagctgg tccgcaggat ccagtcgatc gacggcgtgt tgacgaccga cggcgtgcgg
     8041 ctgccccagg aggccggcct gccggcggat cgctatttcg aggacgagac gcggcaactg
     8101 cagcgcgcgt tcgggctgac ggggatggac ggtgtgccgg cggacgtgcc ggtcatcggg
     8161 gcggcgtacg tcgatcgcac cgactacggc acgccgcggc gcaggccgtc ggccgcggcc
     8221 gaccgtgtgg cgacgccatc gctgaagcgt ccatggtggc gcgtctggta gggggctggc
     8281 catttcccga ggtgtcaggt ctcgcatgcc gcacgatcgg cgaagcagga tcgccgatgt
     8341 ctgggcgtgc ggcattcaag accagacacc tcggcaagtc gaggcgcggc gtcgggcacc
     8401 tcgggcgctg cgacgccgac ttttcaagcc cgcagcttct gcgtctccgg atgccgccgc
     8461 atgtacacgt cgacatagct gcacgacggc tgcacgcgat attccttggc gtcggcccag
     8521 gcgagcgcga cgcgcaccag ctcggcggcg atgccgcggc cgccgacggc gggcggcacg
     8581 ccggtgtgcg tcatccacat gacgttgccg cgcagctggt agtcgcacac gcagagttgg
     8641 ccctcgacgg tggcttcgaa tcgctggcgg gcttcgttgt ggg
//
LOCUS       DATCRO010000779        10693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_425643, whole genome shotgun sequence.
ACCESSION   DATCRO010000779 DATCRO010000000
VERSION     DATCRO010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425643"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1209
                     /locus_tag="VIN75_21250"
     CDS             202..1209
                     /locus_tag="VIN75_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY8976756.1"
                     /translation="MTLTLALLLVSAVVIYLACEFFVNGVEWVGHRFGVSRTAVGTVL
                     AAFGTALPESVVTLVAVVFGKTPDARDIGVGAALGGPLALSTLAYAVVGAMFLIDRAR
                     QHTKIAQEFSTTRLAHDQAWFLGVFVFKVAVGLAAFAWKPWLGWLFLAAYALYVWREL
                     RGETGEGDDASSSEFEPLKLMPRAEHPATIWAVLQTGGALVVIFFASQLFVHQLETVG
                     PWLGLSPQLVALLLSPIATELPEILNAVIWVRQGKTELALGNISGAMMIQATVPSALG
                     IFFTPWLLGAPLLWGAAITMVAIVAMLVLLKRHALTAKRLFAMAALYAVFGVGVALRF
                     H"
     gene            1420..1854
                     /locus_tag="VIN75_21255"
     CDS             1420..1854
                     /locus_tag="VIN75_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976757.1"
                     /translation="MEAMHDATASKEGDMNDGLRRSLLIALAAVLSIALVLTIAVAWY
                     GHGMALAHDRVEAKAAAFCEPIAIGADIASVASSARARRMPVDAATGTTEYRYRFFGG
                     AYWEAQCRVTVDASGRIVAKRAGRAESFAPDRVLTAASAASR"
     gene            complement(1865..2812)
                     /locus_tag="VIN75_21260"
     CDS             complement(1865..2812)
                     /locus_tag="VIN75_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY8976758.1"
                     /translation="MCLVALAIDENRRFPLVIATNRDEFFERPAARMGWWTPAEGGPE
                     ILAGRDLHAGGTWLGLSAAGRLALVTNVRRPQDTDPEAPSRGGIVPHWLRTELRPDQF
                     WMQTSLSGYAPFNLIAADFRRGDCFFASNDLPIPRRIERGIFGLSNATLDTPWPKVQK
                     LKARLGEALKTAESVDGLSSQLFTALADRDTFPDDQLPSTGIGRDRERVLSSAFIRTP
                     DGAYGTRCSTIIITEKIKRQLVTHVFERTFTAGPGLALLRRTVLKDWPPRYADRAPQT
                     VETTVVAETEDDGQSVPVLEMPIPVKKTRVRSLLKPISR"
     gene            complement(2800..3207)
                     /locus_tag="VIN75_21265"
     CDS             complement(2800..3207)
                     /locus_tag="VIN75_21265"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEY8976759.1"
                     /translation="MDRTAGLMVCVYYRVANADAARVISDVREFQRTLRPATGVNSSE
                     VLLRCDLPSPASIASLPDARAPSLHPAEPPVADPGADATVMETYRLSPPPAGPDAEAA
                     LRAFLARLEAASRPHASLLRGARHVELFAPCAS"
     gene            complement(3248..4219)
                     /locus_tag="VIN75_21270"
     CDS             complement(3248..4219)
                     /locus_tag="VIN75_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY8976760.1"
                     /translation="MSPTVTASSDLALGAPVAVRLADWGVIRAKGDDAATFLNGQLTQ
                     ETIKLDLGQARLAGYCSPKGRLLASFVMWRAAPDEILLACSADLLAPTLKRLSMFVLR
                     AKCKLTDASSEIALWGVALPGLLHEDAAHSVPAAPYAVSAVAGGTAVRLADAAGATRV
                     LWAAAPDATPPWPTTSSPDVWNWLEVQSGVPRIVASTVEAFVPQMVNLELVGGVNFQK
                     GCYPGQEIVARSQYRGTLKRRMVLAEGAAAATAGAEVFAAADPEQPAGQIVLSASLPH
                     PEGGARHAVLVEVKRALAQAGELHVGAADGPVVRLRALPYEIPADNG"
     gene            4291..5286
                     /gene="mltG"
                     /locus_tag="VIN75_21275"
     CDS             4291..5286
                     /gene="mltG"
                     /locus_tag="VIN75_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8976761.1"
                     /translation="MLKRLFLLLLVLAAGAAGAAFWWLQHPLKLAHDDVEVSIEPGTP
                     VREIVRLWHDAGVDAPPELLYQWFRWSGDAKRIRAGSYLVSQGATPRSLLEKMVRGDE
                     ELESVHIIEGWTVRQMRAALAAAPDLKPTTKDMSDDALMAAIGAPAGEKAEGRFFPDT
                     YVYSKGVKDILVLKRAHDALERQLQAAWALRAADSPLKSPAEALTLASVIEKETGAAS
                     DRGKVGAVFSNRLRIGMPLQSDPTVIYGLGAAYDGSLHKIDLQTDTPYNSYTRPGLPP
                     TPIALPGRDALRAAVRPEPIKALYFVSRGDGTSVFSETLNAHNAAVNQYIRGGHR"
     gene            5283..5906
                     /gene="tmk"
                     /locus_tag="VIN75_21280"
     CDS             5283..5906
                     /gene="tmk"
                     /locus_tag="VIN75_21280"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8976762.1"
                     /translation="MTTGRFVTFEGIDGAGKSSHIAALADWARARSAEVVLAREPGGT
                     PLAEKLRGLFLHDAMDPLTEALLVFAARRDHLRAVIEPALARGALVLCDRFTDSTFAY
                     QGAGRGFDGAVLGQLEAWVQEGRQPDLTLWFDVDPAVAAQRRAAARDADRLEREDEAF
                     FARVRGGYASRAQAAPARFARIDASQPLEAVRADAIAALASRNWYPA"
     gene            5969..6994
                     /locus_tag="VIN75_21285"
     CDS             5969..6994
                     /locus_tag="VIN75_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY8976763.1"
                     /translation="MSATDDSTLPWIAGPARDIATRQRGHALLLQGNAGAGVFELALR
                     VAHTWLCETGGADAPCGRCTACHLLATNSHPDFLALLPELTQVELGWSAGGNDAPEAG
                     DGEGKAKRKPSREIRIEAVRDAIGWTQKSSSRGRAKVLMLFPADAMNLVASNALLKTL
                     EEPPEGVRLLLVAEDAERLLPTLRSRCQRLPFEGPTPGQALAWLEGQGAANAPVLLAA
                     AGGQPLAARRLAAEGFDAAAWIALPKRVADGRWEPPAGATIPRLREVLQKLCHDAMAV
                     AAGSEPRYFPASSLPPAAAWAKLADWSQSLARLARHDEHPFNAPLIIDSLLAEAKAVW
                     AKPRRAG"
     gene            complement(7194..7433)
                     /locus_tag="VIN75_21290"
     CDS             complement(7194..7433)
                     /locus_tag="VIN75_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8976764.1"
                     /translation="MTQPRRAPRLPVVDSDRCTGCGRCVAACEPRVISLETRPDWRKS
                     AVLHQPDGCTGCSLCALRCPFGAIEMRRPSDGVRS"
     gene            complement(7430..7708)
                     /locus_tag="VIN75_21295"
     CDS             complement(7430..7708)
                     /locus_tag="VIN75_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY8976765.1"
                     /translation="MTHIVLHLRIQGLVQGVSYRWNMVQAARQRGVSGWVRNRHDGSV
                     EAMVAGPQPALDDLVAWARRGPPGARVDQVDVEPADGAFDGFEQRPSA"
     gene            7936..8379
                     /locus_tag="VIN75_21300"
     CDS             7936..8379
                     /locus_tag="VIN75_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976766.1"
                     /translation="MKIVRTLAVSAAVVLAMFGSGASAQDSSYTPGTVWNFSYITIEP
                     GQFENYLDYLSKTWRKGQDFAVKEGDIVSYHVFQINNARAGEPDLILAVETKDYLTTA
                     QQMAVQKKFEKFMAQDTHKIDAGMGERKVMRKLSGSMELQELKLK"
     gene            8425..9285
                     /locus_tag="VIN75_21305"
     CDS             8425..9285
                     /locus_tag="VIN75_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976767.1"
                     /translation="MPPPAHRIVPADAHAVPAPFRALAVRSDEPCHAFDARAPYGCVH
                     VVAGDVVDAAARARLAEAGYARVDIDEPFAVDYAATSWDELAAIAAMPDLADAVARAL
                     RMTRVLGDDPADYRRSVATRVDWLASRGAGFHNDVARHWSRSLFWILALDVGDVEFVM
                     PHAALRLPLRDGDLVVFDQVMAHGLCRPGDAGRAVAGHFEDGDAGRQLFLTGEMLLDD
                     ARWAALGAPWQPVEAHAARGALDLMVAQFDDRSGAVQRARSLEHAMSRDTCHVEETSP
                     AAHGSHGDAS"
     gene            9302..10582
                     /locus_tag="VIN75_21310"
     CDS             9302..10582
                     /locus_tag="VIN75_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY8976768.1"
                     /translation="MPLIRSRTWHHAATLLAVALTGCASPPSTSPQPPAITPAAAQAA
                     GVSMDELAQQQGFARWVAGFEDSARAAGVDDATLHQAFDDARYVPRAITSDRAQPEFT
                     RSVWDYLDLIVSPARVQRGQQKLAEVRPQLDAATAQYGVPQATVVAIWGMESDYGANF
                     GDIPTIDALATLGFEGRREAWARQQLMASLKILQGGDIDRAHMIGSWAGAMGQTQFIP
                     SAFLQYAVDADGDGKRDIWGSMADVTASTANFLARSGWQPGQAWGAEVRLPDGFDAGR
                     ADDALRQPSAQWAGEGVQTLDGSPLPELPDGAIFLPAGARGPAFLVGANFRTLLRYNN
                     STSYALAVSLLAQRIAGGPGVQAAWPRDLQPLTRDQLKALQHALNDRGLDAGDVDGQI
                     GPATRQALRSWQRSRNLPADGFPTLDQLAQLQAP"
BASE COUNT         1474 a         3773 c         3974 g         1472 t
ORIGIN      
        1 cggaaatcct cacttgaatc tcgatcacga caacgcgccg tcggcggcgt cggctctcgc
       61 gcgcgtgcgc atgggcgcgc gccagcatgc gccgctgaat gttcttgcgc gtcagagagc
      121 gtcatgcgcg aacacgcacg cacgagcggg cgtgcccccg cgagtgcacg cggccgcgac
      181 gcccgcctcg gggggctcgc gatgaccctc accctcgcac tcctcctggt ctccgccgtc
      241 gtcatctacc tggcctgcga attcttcgtc aacggcgtcg aatgggtcgg ccatcgcttc
      301 ggcgtctctc gcaccgcggt cggcaccgtc ctggccgcgt tcgggacggc gttgcccgag
      361 agtgtggtca cgctggtggc cgtcgtgttc ggcaagacgc cggatgccag ggacatcggc
      421 gtcggggccg cgctgggcgg gccgctcgcg ttgtcgacgc tggcatatgc ggtggtcggc
      481 gcgatgttcc tgatcgatcg cgcgcggcag cacacgaaga tcgcgcagga gttcagcacc
      541 acgcggctcg cgcacgacca ggcgtggttc ctgggcgtgt tcgtcttcaa ggtggcggtc
      601 ggcctggccg cgttcgcgtg gaagccctgg ctgggctggc tgttcctggc ggcctatgcg
      661 ctgtacgtgt ggcgcgagct gcgcggcgag acaggcgagg gcgacgacgc atcgagcagc
      721 gagttcgagc cgctgaagct gatgccgcgg gccgagcatc cggccacgat ctgggccgtg
      781 ctgcagacgg gcggggcgct ggtcgtcatc ttcttcgcgt cacagctgtt cgtgcaccag
      841 ctcgagacgg tcgggccgtg gctgggcctg tcgccgcagc tggtggcgct gctgcttagc
      901 ccgatcgcca cggagttgcc cgagatcctc aacgcggtga tctgggtgcg ccagggcaag
      961 accgagctgg cgctgggcaa catcagcggg gcgatgatga tccaggcgac ggtgcccagc
     1021 gcgctgggga tcttcttcac gccgtggctg ctgggggcgc cgctgctgtg gggtgcggcg
     1081 atcacgatgg tggcgatcgt ggcgatgctg gtgctgctga agcgccatgc gctgacggcg
     1141 aagcggttgt tcgcgatggc ggcgttgtat gcggtgttcg gggtcggcgt ggcgctgcgg
     1201 ttccattgag ccgagcccgc ggcgacgcgg ttgttcgtca tcgtgaaccg gcttctgtcg
     1261 gcagtgcagg cagcgaagcg aatggcgggt tacgcctgcg gctaacccgc cctacgtgac
     1321 cgatgcgccg aggcgcttcg tcgctgcagg ccggtcttcg tggcggcatg cttgcgatcc
     1381 gtcgcaccct gcgagcgcgg ggcgcctcat cgctcgatca tggaggccat gcacgatgcg
     1441 accgcatcga aggagggcga catgaacgac ggactacgac gaagcctgct gatcgcgctg
     1501 gcagcggtgc tgtcgatcgc gctggtcctg acgatcgccg tcgcctggta cggccacggc
     1561 atggcgctgg cgcatgaccg ggtcgaggcg aaggctgcgg cgttctgcga gccgatcgcg
     1621 atcggcgctg acatcgcttc cgtggcgagc agcgcgcgag cgaggcgcat gccggtcgat
     1681 gccgccacgg ggacgaccga gtatcgctat cgcttcttcg gcggcgccta ctgggaggcg
     1741 caatgccgcg tgacggtgga cgcctcgggc cgcatcgtcg ccaagcgcgc cggccgggcc
     1801 gagtcgttcg cgccggaccg ggtgctcacc gccgcgtcgg cggcaagccg ctagccgctg
     1861 gacgctagcg gctgatcggc ttgagcaggc tgcgcacccg cgtcttcttc accgggatcg
     1921 gcatctcgag gacaggcacg ctctggccgt cgtcctcggt ctccgcgacg accgtcgtct
     1981 cgaccgtctg gggcgcgcgg tcggcgtagc gcggcggcca gtccttgagc accgtgcggc
     2041 gcagcagcgc caggccgggg ccggcggtga aggtgcgttc gaagacgtgc gtcacgagct
     2101 ggcgcttgat cttctcggtg atgatgatcg tggagcagcg cgtgccgtac gcgccgtcgg
     2161 gcgtgcggat gaacgcggag ctcagcacgc gttcgcggtc gcggccgatg ccggtgctcg
     2221 gcagctggtc gtcggggaag gtgtcgcggt cggcgagcgc ggtgaacagc tggctggaca
     2281 ggccgtcgac ggactcggcc gtcttcaggg cctcgcccag gcgcgccttg agcttctgca
     2341 ccttgggcca gggcgtgtcg agcgtcgcgt tggacaggcc gaagatgccg cgctcgatgc
     2401 ggcgcgggat cggcaggtcg ttgctggcga agaagcagtc gccgcggcgg aagtcggcgg
     2461 cgatcaggtt gaacggggcg tagccggaca gcgaggtctg catccagaac tggtcggggc
     2521 gcagctcggt gcgcagccag tgcggcacga tgccgccgcg gctgggcgcc tcggggtcgg
     2581 tgtcctgcgg gcggcgcacg ttggtgacca gggccaggcg cccggcggcc gacaggccca
     2641 gccaggtgcc gccggcgtgc aggtcacggc cggccaggat ctccgggccg ccctcggcgg
     2701 gcgtccacca gcccatgcgg gcggccggcc gttcgaagaa ctcgtcgcgg ttggtggcga
     2761 tcaccagggg gaaacggcgg ttctcgtcga tcgcgagggc tacgaggcac atggggcaaa
     2821 cagttcgacg tggcgggcgc cgcgcagcag gctggcgtgc ggccgcgagg ccgcctccag
     2881 ccgggccagg aaggctcgca gcgcggcttc cgcgtcgggg ccggcgggcg gcggcgacag
     2941 gcggtaggtc tccatcacgg ttgcgtccgc gccggggtcg gcgacgggcg gttctgcggg
     3001 gtggagggac ggcgcacggg catcaggcaa cgaagcgatg gaagccgggg agggcaggtc
     3061 gcatcgtaaa agcacttccg acgagttgac gcccgtggcc gggcgcaatg tgcgctggaa
     3121 ttcacgcaca tccgaaatga ctcgcgccgc gtcggcattg gcgacacggt agtagacgca
     3181 gaccatcagc ccagccgtgc ggtccatcac gctttcaccc cgctgaacgg gctcctgcaa
     3241 cgcactatca gccgttgtcg gccggaatct cgtacggcag cgcgcgcagc cgcacgaccg
     3301 ggccgtcggc cgcgcccacg tgcagttcgc cggcctgcgc gagcgcgcgc ttgacctcga
     3361 ccagcaccgc atggcgcgcg ccgccctcgg ggtgcggcag gctggccgac agcacgatct
     3421 ggccggccgg ctgctcgggg tcggcggcgg cgaagacctc ggcccccgcc gtcgcggcgg
     3481 ccgcgccctc ggccagcacc atgcggcgct tgagcgtgcc gcggtactgg ctgcgcgcga
     3541 cgatctcctg gccggggtag cagcccttct ggaaattcac gccgccgacc agttcgaggt
     3601 tgaccatctg cggcacgaag gcctcgacgg tgctggccac gatgcgcggc acgccggact
     3661 gcacctcgag ccagttccag acgtccgggc tggacgtggt gggccacggc ggcgtggcgt
     3721 cgggcgcggc ggcccacagc acgcgcgtgg cgcccgcggc atcggccagg cgcacggcgg
     3781 tgccgccggc gacggccgag accgcgtacg gcgcggccgg gacggaatgc gcggcgtcct
     3841 cgtgcagcag gccgggcagg gcgacgcccc acagcgcgat ctccgacgac gcgtcggtca
     3901 gcttgcactt ggcgcgcagc acgaacatcg acaggcgctt gagcgtgggg gcgagcaggt
     3961 cggcgctgca ggccaggagg atctcgtcgg gcgcggcgcg ccacatcacg aagctggcca
     4021 gcagccggcc cttgggcgag cagtagccgg ccaggcgcgc ctgtccgagg tcgagcttga
     4081 tcgtctcctg cgtcagctgg ccgttgagga acgtggcggc gtcgtcgccc ttggcgcgga
     4141 tgacgcccca gtcggcgagg cggacggcga cgggggcgcc cagggcgaga tcggaagaag
     4201 cggtaacagt cgggctcatg ggcgcgatta tcatggcggc ccgtctttcc catcgccagc
     4261 agggcctccg atcgagggcg cccgcgatcc atgctcaaac gcctgttcct gttgctgctc
     4321 gtgctcgccg cgggggccgc cggcgccgcc ttctggtggc tgcaacatcc gctcaagctc
     4381 gcgcacgacg acgtcgaggt ctccatcgag cccggcacgc cggtgcgcga gatcgtgcgg
     4441 ctgtggcacg acgcgggcgt cgacgcgccg cccgagctgc tctaccagtg gttccgctgg
     4501 tcgggcgacg ccaagcgcat ccgcgcaggc agctatctcg tctcgcaggg cgcgacgccg
     4561 cgttcgctgc tcgagaagat ggtgcgcggc gacgaggaac tggagtcggt gcacatcatc
     4621 gagggctgga cggtcaggca gatgcgcgcc gcgctcgccg ccgcgccgga cctgaagccg
     4681 acgacgaagg acatgagcga cgacgcgctg atggccgcga tcggcgcgcc ggccggcgag
     4741 aaggccgaag ggcgcttctt ccccgacacc tacgtctaca gcaagggcgt caaggacatc
     4801 ctcgtgctca agcgcgcgca cgacgcgctc gaacgccagc tgcaggccgc ctgggcgctg
     4861 cgcgcggccg acagcccgct gaagtcgccc gccgaggcgc tgacgctggc ctcggtcatc
     4921 gagaaggaga cgggcgcggc gtccgaccgc ggcaaggtgg gcgcagtgtt ctccaaccgg
     4981 ctgcgcatcg gcatgccgct gcagtcggat ccgacggtca tctacggcct gggcgcggcc
     5041 tacgacggca gcctgcacaa gatcgacctg cagacggaca cgccctacaa ctcgtacacc
     5101 cggccgggcc tgccgccgac gcccatcgcg ctgccgggcc gcgatgccct gcgcgcggcg
     5161 gtgcggcccg agccgatcaa ggcgctgtat ttcgtgtcgc gcggcgacgg gacgagcgtc
     5221 ttcagcgaga cgctcaacgc ccataatgcc gcggtgaacc agtacatccg cggcggccac
     5281 cgatgaccac gggccgcttc gtcaccttcg agggcatcga cggggccggc aagtcgtcgc
     5341 acatcgcggc gctggccgac tgggcccgcg cgcgcagcgc cgaggtggtg ctggcgcgcg
     5401 agcccggcgg cacgccgctg gccgagaagc tgcgcgggct gttcctgcac gacgcgatgg
     5461 atccgctgac cgaggcgctg ctggtcttcg ccgcgcggcg cgaccacctg cgcgccgtca
     5521 tcgagccggc gctggcgcgt ggcgcgctgg tgctgtgcga ccgcttcacc gattcgacct
     5581 tcgcctacca gggcgccggc cgcggcttcg atggcgcggt gctcggccag ctcgaggcct
     5641 gggtgcagga agggcgccag cccgatctca cgctgtggtt cgacgtcgat ccggccgtcg
     5701 ccgcgcagcg ccgcgccgcg gcccgcgacg ccgaccgcct cgagcgcgag gacgaggcct
     5761 tcttcgcgcg ggtgcgcggc ggctacgcgt cacgcgcgca ggcggcgccc gcgcgtttcg
     5821 cgcgcatcga cgcgtcgcag ccgctggagg cggtgcgcgc ggatgcgatc gcggcgctgg
     5881 cgtcgcgcaa ctggtatccc gcatgagcgc ccgcccggcc gctccgaagg gcgctcgcac
     5941 cgcagcccgc aggggcggag gttgccttat gagcgccacc gacgattcca cgctgccctg
     6001 gatcgccggc ccggcgcgcg acatcgcgac gcgccagcgc ggccacgcgc tgctgctgca
     6061 gggcaatgcc ggcgcagggg tgttcgaact ggcgctgcgc gtcgcgcaca cctggctgtg
     6121 cgagaccgga ggcgccgacg cgccgtgcgg ccgctgcacc gcctgccacc tgctggccac
     6181 caacagccat cccgatttcc tggcgctgct gccggagctg acgcaggtcg agctcggctg
     6241 gagcgcgggc ggcaacgatg cgcccgaggc gggcgacggc gagggcaagg ccaagcgcaa
     6301 gcccagcagg gagatccgca tcgaggccgt gcgcgacgcg atcggctgga cacagaagag
     6361 ctcgtcgcgc ggccgcgcca aggtgctgat gctgttcccc gccgatgcga tgaacctcgt
     6421 cgcctccaac gccctgctca agacgctgga ggagccgccc gaaggggtgc gcctgctgct
     6481 ggtcgccgag gacgccgaac gcctgctgcc cacgctgcgc agccgctgcc aacgcctgcc
     6541 gttcgagggc ccgacgcccg gccaggcgct cgcgtggctc gaagggcagg gcgccgccaa
     6601 cgcgccggtg ctgctggccg cggccggcgg ccagccgctc gcggcgcgcc gcctcgccgc
     6661 cgaaggtttc gacgccgcgg cctggatcgc gcttccgaag cgcgtcgccg acggccgctg
     6721 ggagccgccc gccggcgcga ccatcccgcg cctgcgcgag gtgctgcaga agctgtgcca
     6781 cgacgcgatg gccgtggccg ccggctccga gccgcgctac tttcccgcga gcagcctgcc
     6841 gcccgcagcg gcgtgggcca agctcgccga ctggtcgcag tcgctcgccc gcctcgcgcg
     6901 ccacgacgag cacccgttca acgccccgct catcatcgac agcctgctgg ccgaggccaa
     6961 ggccgtgtgg gcgaagccgc gccgggcggg ctgaggcgcc gtcctgccgt ggcatccgct
     7021 ggtgtcaggt cttgaaacgt gcagtgcgcg tttggggtca ggtcttgaat gttgcggggt
     7081 caggtcttga atatagcttc gaccggtcgt gcaggcgtgg cgacttcgat gcgctgaacg
     7141 cgagtcgacc ggcggccaca aactgcacga ttcaagacct gaccccgcaa cattcaagac
     7201 ctgaccccgt ccgaaggtcg gcgcatctcg atcgccccga aggggcagcg cagcgcgcac
     7261 aggctgcagc cggtgcagcc gtcgggctgg tgcaggacgg cggacttgcg ccagtcgggc
     7321 cgggtctcca ggctgatgac gcgcggctcg caggcggcga cgcagcggcc gcaaccggtg
     7381 cagcggtcgc tgtcgacgac cggcaggcgg ggggcgcggc ggggctgggt catgcgctgg
     7441 ggcgttgctc gaagccgtcg aacgcgccat ccgcgggctc gacgtccacc tggtcgacgc
     7501 gcgcgcccgg cgggccgcgg cgggcccagg cgacgaggtc gtcgagcgcg ggctgcgggc
     7561 cggcgaccat ggcctcgacg gagccgtcgt gccggttgcg cacccagccg gacacgccgc
     7621 gctggcgggc ggcctgcacc atgttccagc ggtagctgac gccttgcacg aggccctgga
     7681 tcctcaggtg gagcacgatg tgggtcatgg cgggaagtcg gtcgtccggc aggcagctta
     7741 acgcagcaag gccggtcgag cgccggtgca gcagctaccc gcgccaggcg gaacaaagcg
     7801 gttgcgcgcg gcggccatct ggcgcaaagt ggcgcccgcc gccccgtcgg cagcacgggc
     7861 cggccggaac gcgaacgctg ccggcgcacg tcgcgccaac gcagtcaccg cgtgactggt
     7921 gacggaggga ttgccatgaa gatcgtccgc accctggcgg tgtccgccgc cgtcgtcctg
     7981 gccatgttcg ggtccggcgc gtcggcccag gactccagct acacgccggg gaccgtctgg
     8041 aacttcagct acatcaccat cgagcccggc cagttcgaga actacctgga ctacctgtcc
     8101 aagacctgga ggaagggcca ggacttcgcg gtcaaggagg gcgacatcgt ctcctaccac
     8161 gtgttccaga tcaacaacgc gcgcgccggc gagcccgacc tgatcctggc cgtcgagacc
     8221 aaggactacc tgaccaccgc gcagcagatg gccgtgcaga agaagttcga gaagttcatg
     8281 gcccaggaca cgcacaagat cgacgccggc atgggcgagc gcaaggtcat gcgcaagctc
     8341 tcgggcagca tggagctgca ggagctcaag ctcaagtagg cccgccgacg agggcggcgc
     8401 gcggcctgcc gcgacaatgg ccggatgccg ccgcccgccc atcgcatcgt gcccgccgac
     8461 gcgcacgccg tgcccgcacc attccgggca ttggcggtgc gcagcgacga gccgtgccac
     8521 gcgttcgacg ctcgcgcccc gtacgggtgc gtgcacgtgg tggccggcga cgtcgtcgac
     8581 gcggccgccc gcgcgcgcct ggccgaggcg ggctacgcgc gggtggacat cgacgagccg
     8641 ttcgccgtgg actacgccgc cacgtcctgg gacgagctcg cggcgatcgc cgccatgccc
     8701 gatctggcgg acgcggtcgc gcgcgccctg cgcatgacgc gcgtgctcgg ggacgacccg
     8761 gccgactacc ggcgcagcgt cgcgacgcgc gtcgactggc tcgcgtcgcg cggcgcgggc
     8821 ttccacaacg acgtcgcgcg ccactggtcg cgcagcctgt tctggatcct ggcgctcgac
     8881 gtcggcgacg tggagttcgt catgccgcac gcggcgctgc gcctgccgct gcgcgacggc
     8941 gacctcgtcg tgttcgacca ggtgatggcg cacggcctgt gccggccagg cgatgcgggg
     9001 cgcgcggtgg ccgggcattt cgaggacggc gacgccggcc gccagctgtt cctcaccggc
     9061 gagatgctgc tggacgacgc gcgctgggca gcgctcggcg cgccgtggca gccggtcgag
     9121 gcgcacgcgg cgcgcggcgc gctcgacctg atggtggccc agttcgacga ccgcagcggc
     9181 gcggtccagc gcgcgcgctc gctcgagcac gccatgtcgc gcgacacctg ccacgtggag
     9241 gaaacttccc cggccgcgca cgggtcacac ggcgatgcat cctgacggcg agaatccccg
     9301 catgcccctg atccgttccc gcacctggca ccacgccgcc acgctgctgg cggtcgcgct
     9361 gaccggctgc gcgagtccgc cgtccacgtc gccgcagccg ccggccatca cgcccgcggc
     9421 ggcgcaggcg gccggcgtgt cgatggacga gctcgcgcag cagcagggct tcgcgcgctg
     9481 ggtggcgggc ttcgaggatt cggcgcgcgc cgccggcgtc gacgacgcga cgctgcacca
     9541 ggccttcgac gacgcgcgct acgtgccgcg cgcgatcacg tccgaccgcg cacagcccga
     9601 gttcacgcgt tccgtgtggg actacctcga cctgatcgtg tcgccggcgc gcgtgcagcg
     9661 cgggcagcag aagctcgccg aagtccggcc gcagctcgac gccgccaccg cgcaatacgg
     9721 cgtgccgcag gccaccgtcg tcgcgatctg gggcatggag agcgactacg gcgccaactt
     9781 cggcgacatc cccaccatcg acgcgctcgc cacgctcggc ttcgagggcc gccgcgaggc
     9841 gtgggccagg cagcagctga tggcttcgct caagatcctc cagggcggcg acatcgaccg
     9901 cgcgcacatg atcggctcgt gggccggcgc gatgggccag acgcagttca tcccgagcgc
     9961 cttcctgcag tacgcggtcg acgcggacgg cgacggcaag cgcgacatct ggggcagcat
    10021 ggccgacgtc acggcgtcga ccgcgaactt cctcgcgcgc tcgggctggc agcctggcca
    10081 ggcctggggc gccgaggtga ggctgcccga cggcttcgac gccggccgcg ccgacgatgc
    10141 gctgcgccag ccgagcgcgc aatgggcggg cgagggcgtg cagacgctgg acggcagccc
    10201 gctgcccgag ctgcccgatg gcgccatctt cctgcccgcc ggcgcgcgcg gcccggcctt
    10261 cctggtcggc gcaaactttc gcaccctgct tcgctacaac aactccacca gctacgcgct
    10321 ggccgtgagc ctgctggcac agcgcatcgc cggtggcccg ggcgtgcagg ccgcgtggcc
    10381 gcgcgacctc cagccgttga cgcgcgacca gctcaaggcg ctgcagcacg cgctcaacga
    10441 ccgcggcctg gacgccggcg acgtcgacgg ccagataggc ccggccacgc gccaggccct
    10501 gcgctcgtgg cagcgcagcc ggaacctgcc ggccgacggc ttcccgacgc tggaccagct
    10561 cgcgcaactg caggccccat gacgccaccg acgccgccga cgatcgttcc ggctcgcgcc
    10621 gaggacgcgc ccgcgatgct ggcgctgcag aaggtcgcct tcgagccgga ggcgcgcgcc
    10681 tgcggctcgt ggg
//
LOCUS       DATCRO010000780         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_515692, whole genome shotgun sequence.
ACCESSION   DATCRO010000780 DATCRO010000000
VERSION     DATCRO010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515692"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..>2348
                     /locus_tag="VIN75_21315"
     CDS             217..>2348
                     /locus_tag="VIN75_21315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8976769.1"
                     /translation="MSTSLRVPRSSAEATTSTPRSLPQAEWATWRRLVQVYQDRDTES
                     AEIRARLVQWVTFVSPWLMGCNALSAVLLAWALPVAVAPWLHWSWCGSICALCLFGLR
                     NWWRHRRKVPTRVSPVVVRKAMLHAGALAALWAALPWPVFADGAPSAQILIAGVMLGL
                     VAGGGFVLTPLAPAAIVFAGIIGLSTSAAVVRMHDPMALLFSVLMAGYTIVTIAAVIL
                     GARRSAGALRAEREADRQGQLVALLLKDFESQSADVMWETDAAGRLVRVTPKLTALMR
                     LEDDAAGKRALLGWFERHATDAEAKAAVAKLRQAFDAGQAFRQRGIPVAVAGARRHWS
                     ITARPLVDESGVDQGWRGVITDITMEHDAQSRMQQLALVDSLTGLANRGQLRDKLQLK
                     IASAAQRPAALLCLDMDHFKRINDTFGHATGDVVLRETARRLRKLVRASDIVARAGGD
                     EFAVLLDDIDGEQDALRFGQRVVAEINRAFSSDAGAINASASVGVVAIPGHGTSVDEL
                     LANADLALGSAKAGGRARAEIFHADMGERLRLRMTLERDLRQAVARKELRLHWQPQVD
                     TSQWRISGCEALLRWQHGEQGLIPPMNFIPLAEETGLIVELGEWVLNEACRVGARKLP
                     GLMVAVNVSPIQVVRDDFVGVVRKALAAGGLPPQRLEIEITESLFIDASPKALRNLET
                     LRQMGIRVALDDFGTGYSSLAYLRQFPF"
BASE COUNT          302 a          850 c          849 g          347 t
ORIGIN      
        1 cccgggggac agagcggcca cccgatgtcg ccgtggtacg gcgccgggta tgccgactgg
       61 gtcgcgggcc ggcccgtgcc gctgctcgcg ggacccgctc gccatgtcct cgagctcgat
      121 ccctgagcag tcgatcagtg cgacttttcg ggcaaacccg ggcgcgggcg cgtcaagttc
      181 gggcagggcg ggccgatacc cgaatgaatc cccgccatgt ccacgagcct ccgcgtgccc
      241 cgttcctccg ccgaagcgac gacctcgacg ccccgctccc tgcctcaggc cgagtgggcg
      301 acgtggcgcc gcctggtcca ggtctaccag gatcgcgaca ccgaatcggc cgagatccgc
      361 gcgcgcctcg tgcagtgggt caccttcgtc tcgccgtggc tgatgggctg caacgcgctc
      421 agcgccgtgc tgctcgcgtg ggcgctgccg gtggccgtcg cgccgtggct ccactggagc
      481 tggtgcggct cgatctgtgc gctgtgcctg ttcggcctgc gcaactggtg gcgccatcgc
      541 cgcaaggtgc ccacgcgcgt cagcccggtc gtggtgcgca aggccatgct gcatgccggc
      601 gcgcttgccg cgctgtgggc cgcgctgccg tggcccgtgt tcgccgacgg cgcgccgtcc
      661 gcgcagatcc tgatcgccgg cgtgatgctg ggcctggtgg ccggcggcgg cttcgtgctg
      721 acgccgctgg cgccggccgc gatcgtcttc gcgggcatca tcggcctgtc caccagcgcg
      781 gccgtcgtgc gcatgcacga cccgatggcg ctgctgttct cggtgctgat ggccggctac
      841 acgatcgtga cgatcgccgc cgtgatcctg ggcgcgcgcc gctcggccgg cgcgctgcgc
      901 gccgagcgcg aggccgatcg ccagggccag ctcgtggcgc tgctgctcaa ggacttcgaa
      961 tcgcagtcgg ccgacgtcat gtgggagacc gacgcggcgg gccgcctcgt gcgcgtcacg
     1021 cccaagctca ccgcgctcat gcgcctggaa gacgacgccg ccggcaagcg cgccttgctg
     1081 ggctggttcg agcggcatgc caccgacgcc gaggccaagg ccgcggtcgc caagctgcgc
     1141 caggccttcg atgccggaca ggcgttccgc cagcgcggca tcccggtcgc cgtcgccggc
     1201 gcgcggcggc actggtcgat tacggcgcgt ccgctggtcg acgagagcgg cgtcgaccag
     1261 ggctggcgcg gcgtcatcac ggacatcacc atggagcacg acgcgcagtc gcgcatgcag
     1321 cagctcgcgc tcgtggactc gctcacgggc ctggccaacc gcgggcagtt gcgcgacaag
     1381 ctgcagctca agatcgcgtc cgcggcgcag cgtccggccg cgctgctgtg cctggacatg
     1441 gatcacttca agcgcatcaa cgacaccttc ggccacgcca cgggcgacgt cgtgctgcgc
     1501 gagacggcgc gccggttgcg caagctggtg cgcgcctcgg acatcgtcgc gcgcgccggc
     1561 ggcgacgagt tcgcagtgct gctggacgac atcgacggcg agcaggacgc gctgcgcttc
     1621 ggccagcgcg tcgtcgccga gatcaatcgc gcgttctcga gcgacgcggg cgcgatcaac
     1681 gcgagcgcca gcgtgggcgt ggtcgccatc cccgggcatg gcacgagcgt cgacgagctg
     1741 ctggccaatg ccgacctggc gctgggcagc gcgaaggccg gcggccgtgc gcgcgccgag
     1801 atcttccacg ccgacatggg cgagcgcctg cgcttgcgca tgacgctcga gcgcgacctg
     1861 cgccaggccg tcgcgcgcaa ggagctgcgc ctgcactggc agccccaggt cgacacgtcc
     1921 caatggcgca tcagcggctg cgaggcgctg ctgcgctggc agcacggcga gcagggcctg
     1981 atcccgccga tgaacttcat cccgctggcc gaggagacgg gcctcatcgt cgagctcggc
     2041 gaatgggtgc tcaacgaggc ctgccgcgtg ggcgcgcgca agctgccggg gctgatggtg
     2101 gccgtcaacg tgtcgccgat ccaggtggtg cgcgacgact tcgtcggcgt cgtgcgcaag
     2161 gcgctcgcgg ccggcggcct gccgccgcag cgactcgaga tcgagatcac ggagtcgctg
     2221 ttcatcgacg cgtcgcccaa ggcgctgcgc aacctcgaga cgctgcgcca gatgggcatc
     2281 cgcgtcgcgc tcgacgactt cggcacgggc tactcgtcgc tcgcctacct gcgccagttc
     2341 ccgttcga
//
LOCUS       DATCRO010000781         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1032690, whole genome shotgun sequence.
ACCESSION   DATCRO010000781 DATCRO010000000
VERSION     DATCRO010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032690"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="tsaD"
                     /locus_tag="VIN75_21320"
     CDS             complement(<1..992)
                     /gene="tsaD"
                     /locus_tag="VIN75_21320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8976770.1"
                     /translation="MIVLGIESSCDETGVALVETTGAGVPRLRSQALHSQIRMHQAYG
                     GVVPELASRDHVRRVLPLLEAALADASLARDDIDVVAFTRGPGLAGALLVGAGVACAL
                     AASMGKPTLGVHHLEGHLMSPFLSADPPQFPFVALLVSGGHTQLMRVDDVGQYEMLGE
                     TIDDAAGEAFDKSAKLLGLGYPGGPALAALAAQGDPASVALPRPLLHSGNLDFSFAGL
                     KTAVATALRKFGPEPTDRQRADLAASTQAAIVEVLVKKSLRALQETRLSRLVVAGGVG
                     ANTRLREHLDGVAARRGLRVHYPELALCTDNGAMIAMAGALRLASGKEKASRSYA"
     gene            1250..1465
                     /locus_tag="VIN75_21325"
     CDS             1250..1465
                     /locus_tag="VIN75_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976771.1"
                     /translation="MSELSQRFVSVRDLARSSGASVRDVEELIAKLEKDGALMTRATP
                     QAIGQAGGGWNQWWPVAAVRDMVRDWR"
     gene            complement(1557..1895)
                     /gene="fdx"
                     /locus_tag="VIN75_21330"
     CDS             complement(1557..1895)
                     /gene="fdx"
                     /locus_tag="VIN75_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY8976772.1"
                     /translation="MTVIKILPHATLCPEGKTIEAAPGTSVCEALLENGVEIEHACDM
                     SCACTTCHVIVREGFDSLNEAEDTEEDLLDRAWGLTAQSRLSCQAIVAAKDLVIEIPR
                     YTINHARENH"
     gene            complement(1898..>2443)
                     /locus_tag="VIN75_21335"
     CDS             complement(1898..>2443)
                     /locus_tag="VIN75_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8976773.1"
                     /translation="VVQGERELVSECRSLARFELRGIPPMVAGAARIRVTFQVDADGL
                     LSVSAKELTSGVEAAIVVKPSYGLSDEQVAGMLKEGFASAEADMAARSLREAQVEAER
                     MVLATESALAADGDLLAAGERAAIDALVARVRAAARDAGEPRAIEAAVEALAEGTEGF
                     AAARMNRGIREALAGKNVNEI"
BASE COUNT          356 a          867 c          849 g          371 t
ORIGIN      
        1 gcgtaggacc ggctggcctt ttccttgccc gacgccagcc gcagcgcccc cgccatcgcg
       61 atcatcgcgc cgttgtcggt gcacagcgcc agctccgggt agtgcacgcg caggccgcgg
      121 cgtgcggcga cgccgtccag atgctcgcgc agtcgcgtgt tggcgccgac gccgccggcc
      181 acgacgaggc gcgacagccg cgtctcctgc aacgcgcgca gcgacttctt gaccagcacc
      241 tcgacgatcg ccgcctgcgt gctcgccgcg aggtccgcgc gctgtcggtc ggtcggctcg
      301 gggccgaact tgcgcagcgc ggtggcgacg gcggtcttca ggccggcgaa cgagaagtcg
      361 aggttgccgc tgtgcagcag cggacgcggc agcgcgaccg atgccgggtc gccctgcgcc
      421 gccagcgcgg ccagggccgg gccgccgggg tagcccaggc ccagcagctt ggccgacttg
      481 tcgaacgcct cgccggccgc gtcgtcgatg gtctcgccca gcatctcgta ctgcccgacg
      541 tcgtccacgc gcatcagctg cgtgtgcccg cccgagacca gcagcgccac gaacgggaac
      601 tgcggcggat cggccgacag gaacggcgac atcaggtggc cctcgaggtg gtgcacgccc
      661 agcgtgggct tgcccatcga ggcggccagc gcgcatgcga cgccggctcc cacgagcagc
      721 gccccggcca ggcccggccc gcgcgtgaag gcgacgacgt cgatgtcgtc ccgcgcgagc
      781 gacgcgtcgg ccagcgcggc ctcgagcagc ggcagcacgc gccgcacatg gtcgcgcgac
      841 gcgagctcgg gcaccacgcc gccgtaggcc tggtgcatgc ggatctgcga atgcagcgcc
      901 tgcgagcgaa ggcgcggcac gccggcgccc gtcgtctcga cgagggcgac gccggtctcg
      961 tcgcaggagg attcgatgcc gaggacgatc atgcgcggtt caagtgtgaa ttagtcgact
     1021 caagtcgtta caacctcgtg tcgagctggc ttccggaaac tgaaatggcc ggtttaaatt
     1081 ctgcccgtgg tgatattcgg cctcacccgt cggaacgacg aatcacattg tcatccgaac
     1141 catacaaacc acacggccgt cgagttcgtt gggaatgatg aagcaataca aactcactgc
     1201 ttggccggag ttgcctgcgc gattccagaa gaccacgatg cgccgcgtcg tgagcgagtt
     1261 gtcccagcgc ttcgtcagcg tccgcgacct ggcccgcagc agcggcgcga gcgtgcgcga
     1321 cgtcgaggaa ctgatcgcga agctcgagaa ggacggcgcg ctgatgaccc gtgccacgcc
     1381 ccaggcgatc ggccaggccg gcggcggctg gaaccagtgg tggcccgtgg cggccgtgcg
     1441 cgacatggtg cgcgactggc gctgagacgc ctcccctcgc gacaaggcgg cccgcgggcc
     1501 gctttttcat ttccgcagtt gtcacgcggc gccgtgtgac aactcggggc cgtcgctcaa
     1561 tgattctcgc gggcgtggtt gatcgtgtac ctgggaatct cgatcacgag atccttggct
     1621 gcgacgatcg cctggcacga cagccgcgat tgcgcggtca ggccccaggc gcgatcgagc
     1681 aggtcctctt cggtgtcctc ggcctcgttg agtgaatcaa agccctcgcg cacgatcacg
     1741 tggcaggtgg tgcaggcgca gctcatgtcg cacgcatgct cgatctcgac gccgttctcc
     1801 agcagcgcct cgcacacgga ggtgccgggc gcggcctcga tggtcttgcc ttcggggcat
     1861 agcgtggcat gcgggaggat cttgatgacg gtcattctca aatctcgttg acgttcttgc
     1921 cggccagggc ttcgcggatg ccgcggttca tgcgcgcggc ggcgaagcct tcggtgccct
     1981 cggccagcgc ctcgacggcg gcctcgatcg cgcgcggctc gcccgcgtca cgcgcggcgg
     2041 cgcgcacgcg cgcgaccagc gcgtcgatcg cggcgcgttc gccggccgcg agcaggtcgc
     2101 cgtccgcagc cagcgccgac tcggtggcca gcaccatgcg ctcggcctcg acctgcgcct
     2161 cgcgcaggct gcgcgcggcc atgtcggcct cggcgctcgc gaagccttcc ttgagcatgc
     2221 cggccacctg ttcgtcgctc agtccgtagc tgggcttgac gacgatcgcg gcctcgacgc
     2281 ccgacgtgag ctccttggcg ctgacgctca gcaggccgtc ggcgtccacc tggaacgtga
     2341 cgcggatgcg cgccgcgccg gccaccatcg gcgggatgcc gcgcagctcg aagcgcgcca
     2401 gcgagcggca ctcggagacg agctcgcgct cgccctgcac cac
//
LOCUS       DATCRO010000782         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_109913, whole genome shotgun sequence.
ACCESSION   DATCRO010000782 DATCRO010000000
VERSION     DATCRO010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109913"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VIN75_21340"
     CDS             <1..123
                     /locus_tag="VIN75_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8976774.1"
                     /translation="VGELVMRELKRLDKVAYVRFASVYRSFEDVDEFSRLIKDI"
     gene            complement(186..653)
                     /locus_tag="VIN75_21345"
     CDS             complement(186..653)
                     /locus_tag="VIN75_21345"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY8976775.1"
                     /translation="MRARSPRHATCRVGHARRGFTLVELLVVAAALAILAAIALPAFF
                     ESLARARRADAQAALLEDAGYMQHYYASHDAFSGVPAPQLPFARTPRQGGVAYVITVA
                     VPPGDPSTFLLTAVRAGGMSRDPCGDFSVDHLGQRSLVPGTFAADRDAARCWR"
     gene            complement(694..1335)
                     /locus_tag="VIN75_21350"
     CDS             complement(694..1335)
                     /locus_tag="VIN75_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976776.1"
                     /translation="MNAPPRSTPARAPRVTRARARGFSLIVAMLMLAVIGLASAAVMR
                     NAVSGDQVANNNRLQTQASQYAQLALRFCQAQLALPPESRVARLLPAATPPAWTVQRN
                     WGGASGDPAHTLSAAEIGASVQPRVAPQCLAEATSLAGTYTVTARGFSADFKADPSTG
                     ATRTGSTVWVQATIYADSRPGAAGEAPAAAGRAPAGPLIVRQRTWQQLLTPPF"
     gene            complement(1332..>2062)
                     /locus_tag="VIN75_21355"
     CDS             complement(1332..>2062)
                     /locus_tag="VIN75_21355"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY8976777.1"
                     /translation="LALNVMRQQVAQAGFSQPHGVGAGGGLVLHAFRAVAGCEATGFA
                     DLQASIAAPGNCQPASNDPSAPDTIEVAYEGSVLPGMSSNGILGGTGTPQPLDCLGNG
                     FAKTHDDATGDDYWLNDSKFYIADGSLYCHGPGNRAGAAIVQNVETLQVTYGMAAAPA
                     GAPGAGQVVYYDNAPPPGSPLWADVVSVKLCVQVRSAAKVLDTASSPTLGGWIDCANA
                     QRASSDGYLRRTFTTTIVLQNKLL"
BASE COUNT          268 a          722 c          806 g          266 t
ORIGIN      
        1 gttggcgagc tcgtgatgcg cgagctcaag cgcctggaca aggtggccta cgtgcgcttc
       61 gcgtcggtct atcgcagctt cgaggacgtc gacgagttca gtcggctgat caaggacatc
      121 tgaggccgtc gcggttcgct acgcgccgga tggcgcatcg gctgacgttc gcttgcgcgg
      181 cgcgatcaac gccagcagcg cgccgcgtcg cggtcggccg cgaaggtgcc gggcaccagc
      241 gagcgctgcc cgaggtggtc gacgctgaag tcaccgcaag ggtcgcgcga catgccgccc
      301 gcccgcacgg cggtcaacag gaacgtcgac ggatcgccgg gcggcaccgc caccgtgatg
      361 acataggcga cgccgccctg gcgcggcgtc cgcgcgaacg gcagctgcgg cgccggcacg
      421 ccgctgaatg cgtcgtgcga ggcgtagtag tgctgcatgt agcccgcgtc ctcgagcagc
      481 gcggcctgcg cgtcggcgcg ccgcgcgcgc gccagcgact cgaagaaggc cggcagcgcg
      541 atcgcggcga ggatggccag cgccgcggcg acgaccagca gctcgacgag cgtgaagcca
      601 cggcgcgcgt gaccgacacg gcacgtggcg tggcgaggcg agcgcgcgcg cagggcgacc
      661 tccggggact cgcgaacgac gtggcgcgcc ggatcagaac ggcggcgtga gcagctgctg
      721 ccacgtgcgc tggcgcacga tcagcggccc cgctggcgcg cggccggcgg ccgcgggcgc
      781 ctcgccggcg gcacccggcc ggctgtcggc gtagatcgtc gcctgcaccc acacggtcga
      841 gccggtgcgc gtcgccccgg tggacgggtc ggccttgaag tcggcactga agccgcgcgc
      901 ggtgacggtg taggtgccgg ccaggctcgt cgcctccgcc aggcactgcg gcgcgacgcg
      961 cggctgcacg ctggcgccga tctcggcggc cgagagcgtg tgggcggggt cgccgctggc
     1021 gccgccccag tttcgctgca ccgtccaggc cggcggcgtg gccgcgggca gcaggcgggc
     1081 gacgcgcgac tcgggcggca gcgccagttg cgcctggcag aagcgcagcg cgagctgggc
     1141 gtactggctg gcctgggtct ggagccggtt gttgttggcg acctggtcgc cgctgacggc
     1201 gttgcgcatg acggccgccg acgcgaggcc gatcaccgcg agcatgagca tcgccacgat
     1261 cagcgagaag ccccgggcgc gagcgcgcgt gacgcgcggc gcgcgggcgg gcgtggagcg
     1321 agggggcgcg ttcacagcag cttgttctgc aacacgatgg tggtggtgaa cgtgcggcgc
     1381 aggtagccgt cgctggacgc gcgctgcgcg ttggcgcagt cgatccagcc gcccagcgtc
     1441 ggcgacgagg ccgtgtccag caccttggcc gcgctgcgca cctgcacgca cagcttgacc
     1501 gacaccacgt cggcccacag cggcgagccc ggcggcggcg cgttgtcgta gtagacgacc
     1561 tggcccgcgc cgggcgcccc ggcgggcgcc gcggccatgc cgtaggtgac ctgcagcgtc
     1621 tcgacgttct gcacgatggc ggcaccggcc cggttgcccg gcccgtggca gtacaggctg
     1681 ccgtcggcga tgtagaactt gctgtcgttg agccagtagt catcgccggt ggcgtcgtcg
     1741 tgcgtcttcg cgaagccgtt gccgaggcag tcgagcggct gcggcgtgcc cgtgccgccc
     1801 aggatgccgt tggacgacat gccgggcagc acgctgccct cgtaggccac ctcgatcgtg
     1861 tcgggggccg acgggtcgtt cgacgccggc tggcagttgc ccggcgcggc gatgctcgcc
     1921 tgcaggtcgg cgaagccggt cgcctcgcag cccgcgacgg cccggaacgc gtgcagcacc
     1981 aggccgccgc cggcgcccac gccatgcggc tggctgaagc ccgcctgcgc gacctgctgg
     2041 cgcatgacgt tgagcgccag cg
//
LOCUS       DATCRO010000783         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_571362, whole genome shotgun sequence.
ACCESSION   DATCRO010000783 DATCRO010000000
VERSION     DATCRO010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571362"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..981)
                     /locus_tag="VIN75_21360"
     CDS             complement(226..981)
                     /locus_tag="VIN75_21360"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY8976778.1"
                     /translation="MTHPKEAPFASLQFYATAPYPCSYLPGRMARSQVATPGHLIHAE
                     AYSALVATGFRRSGVFTYRPYCDRCRACVPMRVPVAGFKPSRSQRRAWAAHAGLKVRV
                     MRLAFVPEHYELYLRYQAGRHAGGGMDHDSVDQYTQFLLQSRVNTRLVEFREPDEGPN
                     PGALRMVAIADILSDGLSAVYTFYDPDLRGSFGTFAVLWQIEQARSLKLPYLYLGYWI
                     AESPKMAYKVQFKPYETLVEGRWRPSQDAPDAA"
     gene            complement(978..1763)
                     /gene="aat"
                     /locus_tag="VIN75_21365"
     CDS             complement(978..1763)
                     /gene="aat"
                     /locus_tag="VIN75_21365"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY8976779.1"
                     /translation="MAIPWLLADSPLPPTSSALDDRSEAPGLLAAGGGLSIARLRDAY
                     SHGVFPWYSPGQPVLWWSPDPRMLLRTADFKLSHSLRKSLRRFLRTPGCELRFDHDVA
                     RVMAHCADTPRDGQDGTWIVPEMQAAYAAWARSGDVHSVETWIDGELVGGLYGVNLGR
                     MMFGESMFSHRTDASKIALAALVAFCRFHDMPAIDCQQATGHLASLGASPVPRAAFER
                     EVDALVSRAPPPVWAYDFRLWDTLDLGLPVPSSTAAQSADLSP"
     gene            complement(1990..2655)
                     /locus_tag="VIN75_21370"
     CDS             complement(1990..2655)
                     /locus_tag="VIN75_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY8976780.1"
                     /translation="MNNLPPLSFAAARSSRLTRLATVALLLGGVAVGAMAQTYKVVGP
                     DGKVTYTDKPPTASAIKAPGPGGDTSSAANGIPYETRQAMAKYPVTLYSTKNCSACDN
                     ARQALRRRGIPFNEYTISDDTDFAAYRSRLNATTFPGISVGSKNLAGFSPTDLSSYLD
                     AAGYPAQTHLSGYTWPPAAPMVPASAKQAAAAEDAGQAPAPAAASAPEPVFPPPSKSG
                     IQF"
     gene            complement(2778..>3788)
                     /locus_tag="VIN75_21375"
     CDS             complement(2778..>3788)
                     /locus_tag="VIN75_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8976781.1"
                     /translation="RYAFSDEEVRQFFTLPKVLDGLFRIIETLFEVRIVKSAAPAWHE
                     SVTFYRIERDGALVGEFFLDLYARAGKRPGAWMDDARQRWLRADGQGLQTPVAHLVCN
                     FAAPVNGRPALLTHDNVITLFHEFGHGLHHMLTQVEELGVSGISGVEWDAVELPSQFM
                     ENFCWEWDVLQQLTEHVDTGATLPRPLFDKMLAAKNFQSGMQTLRQVEFALFDMRIHS
                     EPGSSDRVQAILDEVRAEIAVLVPPSFNRFQHSFSHIFGGGYAAGYYSYTWAEVLSAD
                     AFSAFEESGVLDLDTGRRYRREILEVGGSRDALDSFKAFRGRAPSIDAMLRHLGIARP
                     AA"
BASE COUNT          546 a         1243 c         1469 g          530 t
ORIGIN      
        1 gccgcggcgg tgacggcgag cgtggcgaag gcgtgggtgg cgttggcggt gaacttgcgc
       61 atgtcggctc tccgggaggt cgttgttgaa tcgatggagg cactgtaggc agccggtacg
      121 gccgcgtcgc gcggcaggcg acgtgctggc ccgtgggggc gaccaaacgc cggtcggcgc
      181 gacgaaatgc agggacggcg caccgtgacg gtggcgcgcg gtcgctcagg cggcgtccgg
      241 cgcgtcctgg ctgggccgcc aacggccttc gacgagggtc tcgtagggct tgaactgcac
      301 cttgtaggcc atcttggggc tctcggcgat ccagtagccg aggtacaggt agggcagctt
      361 gagcgagcgc gcctgctcga tctgccacag gacggcgaag gtgccgaagc tgccgcgcag
      421 gtcggggtcg tagaaggtgt agacggcgga cagcccgtcg ctgaggatgt cggcgatggc
      481 caccatgcgc agcgcgcccg ggttcgggcc ctcgtccggc tcgcggaact cgaccagccg
      541 cgtgttgacc cggctttgca gcaggaactg ggtgtactgg tcgacgctgt cgtggtccat
      601 cccgccgccg gcgtggcggc cggcctggta gcgcaggtac agctcgtagt gctcgggcac
      661 gaaggccagg cgcatcaccc gcaccttcag gccggcgtgt gccgcccagg cgcggcgctg
      721 gctgcgcgag ggcttgaagc cggcgacggg cacgcgcatg ggcacgcagg cgcggcagcg
      781 gtcgcaatag ggccggtagg tgaacacccc gctgcggcgg aaaccggtgg ccaccagcgc
      841 cgagtaggcc tccgcgtgga tcaggtgtcc cggcgtggcg acctgcgagc gcgccatccg
      901 cccgggcagg tagctgcacg ggtagggcgc ggtcgcatag aactgcagcg acgcgaacgg
      961 ggcttccttc gggtgggtca cggcgaaagg tcggcggatt gggcggcggt cgacgagggg
     1021 acgggcaagc cgaggtccag cgtgtcccac aggcggaaat cataggccca gaccggtggc
     1081 ggcgcccggg aaaccagcgc atccacctcc cgttcgaagg ccgcgcgcgg caccggcgac
     1141 gcgcccaggc tggccaggtg gccggtggcc tgctggcagt cgatggcggg catgtcgtgg
     1201 aagcggcaga aagccaccag cgccgccagc gcgatcttgg aggcgtcggt gcggtgcgag
     1261 aacatcgact cgccgaacat catccggccg aggttgacgc cgtacaggcc gccgaccagc
     1321 tcgccgtcga tccaggtctc cacgctgtgc acgtcgcccg agcgcgccca ggcggcgtag
     1381 gcggcctgca tctcgggcac gatccaggtg ccgtcctggc cgtcgcgcgg ggtgtcggcg
     1441 caatgggcca tgacccgcgc gacgtcgtgg tcgaagcgca gctcgcagcc cggcgtgcgc
     1501 aggaagcggc gcaatgactt gcgcagcgag tgcgacagct tgaagtcggc cgtgcgcagc
     1561 agcatgcgcg ggtcgggcga ccaccagagc accggctggc ccgggctgta ccaggggaag
     1621 acgccgtgcg agtaggcgtc gcgcagccgg gcgatggaca gcccgccgcc ggccgcgagc
     1681 aggcccgggg cctcggatcg atcgtccagc gcgctggaag tgggtggcag cggggagtcc
     1741 gctagcagcc aggggatcgc catgggagca aggatacgtc gcgcgatcgg gatgcgggcg
     1801 cgtgcgcggc tgctcgtgcg ggcactctgg acggctcgcg tgtgggtcgt cccgcgcgtc
     1861 gtgccgctgg ggcgtcgccg acggcttcag gcgtgccggc gtatcgcccc acgcgggcgc
     1921 gcccgtggcc gcgcaccggc gcgcgcgcct ggcggcgcgc ccgggccggc atcgtgccgc
     1981 gcatgatcct cagaactgga tgccgctctt gctgggcggc gggaacaccg gttcgggcgc
     2041 cgaggcggcg gccggcgcgg gcgcctggcc ggcgtcctcg gcggccgcgg cttgcttggc
     2101 gctggccggc accatgggcg cggccggcgg ccacgtgtag ccgctcaggt gcgtctgcgc
     2161 ggggtagccg gcggcgtcca ggtagctgga caggtcggtc ggcgagaagc ccgcgaggtt
     2221 cttgctgccc accgagatgc ccgggaaggt ggtggcgttc aggcgcgagc ggtacgcggc
     2281 gaagtcggtg tcgtcgctga tcgtgtactc gttgaacgga atgccgcggc ggcgcagcgc
     2341 ctggcgcgcg ttgtcgcagg ccgagcagtt cttcgtgctg tacagcgtca ccgggtactt
     2401 ggccatggcc tggcgcgtct cgtacgggat gccgttggcg gccgacgacg tgtcgccgcc
     2461 cgggcccggg gccttgatgg ccgaggcggt gggcggcttg tcggtgtagg tgaccttgcc
     2521 gtccgggccg acgaccttgt acgtctgcgc catcgcgccg accgcgacgc cgccgagcag
     2581 cagcgccacg gtggccaggc gcgtgaggcg ggacgagcgg gcggctgcga aggagagggg
     2641 gggcaggttg ttcatcgtgg gcatcgtacg aaaggtcagg aaggagtcgc aagtggcggc
     2701 acacccggat caacgcgcca gccggccggc gggacgaccc gcgcgcgccg gcgccgcgcc
     2761 ggggacgcgg cacgccgtca ggccgccggc ctggcgatcc cgaggtggcg cagcatcgcg
     2821 tcgatgctgg gcgcccggcc gcggaaggcc ttgaagctgt cgagcgcgtc gcggctgccg
     2881 ccgacctcga ggatctcgcg gcgatagcga cgcccggtat ccaggtccag cacgcccgac
     2941 tcctcgaacg cgctgaacgc atccgcggac aatacctcgg cccaggtgta gctgtagtac
     3001 ccggccgcgt aaccgccccc gaaaatgtgt gaaaagctgt gctgaaaccg gttgaacgac
     3061 ggcggcacca gcacggcgat ctcggcccgc acctcgtcga ggatggcctg gacgcggtcc
     3121 gacgaaccgg gctccgagtg gatgcgcatg tcgaacagcg cgaactccac ctgccgcagc
     3181 gtctgcatgc cgctctggaa attcttggcg gccagcatct tgtcgaacag cgggcgtggc
     3241 agcgtggcgc cggtgtccac gtgctcggtc agctgctgca ggacgtccca ttcccagcag
     3301 aagttctcca tgaactggct gggcagttcc acggcgtccc attcgacgcc cgagatgccc
     3361 gacacgccca gctcctcgac ctgcgtgagc atgtggtgca ggccgtggcc gaactcgtgg
     3421 aacagcgtga tgacgttgtc gtgcgtgagc agcgcgggac ggccgttcac cggcgcggcg
     3481 aagttgcaca ccaggtgggc gaccggcgtc tgcaggccct ggccgtcggc gcgcagccag
     3541 cgctggcggg cgtcgtccat ccaggcgccg gggcgcttgc cggcgcgcgc gtacaggtcg
     3601 aggaagaact cgccgaccag cgcgccgtcg cgctcgatgc ggtagaacgt cacgctctcg
     3661 tgccaggccg gcgccgccga cttgacgatc ctgacctcga acagcgtctc gatgatgcgg
     3721 aacaggccgt ccagcacctt gggcagcgtg aagaactggc gcacctcctc gtcgctgaac
     3781 gcgtagcg
//
LOCUS       DATCRO010000784         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_54022, whole genome shotgun sequence.
ACCESSION   DATCRO010000784 DATCRO010000000
VERSION     DATCRO010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54022"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VIN75_21380"
     CDS             <1..553
                     /locus_tag="VIN75_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8976782.1"
                     /translation="FQEQHQDTQLMVSWDARSLDLHTKPYDPALGTLALVKLFGQDPQ
                     LSQLGPEALAVFGNYLDYVQVEAGRQVIGQDEQGDFLLIVLDGTVAVERVQPWGAKAR
                     LGESRAGDVLGEMSLLDAGARFSACTTLTPAKFAVLGAAALDRMMQHEPRLAAAMLAW
                     LARRLSLRLRQVSARLSALLTRE"
     gene            665..1801
                     /locus_tag="VIN75_21385"
     CDS             665..1801
                     /locus_tag="VIN75_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8976783.1"
                     /translation="MEREQASKFVNDLLRLMVTRNGSDLFLTADFPPAIKVDGKVTKV
                     SPQPLTGAHTMALARAIMNDKQASDFERTKECNFAISPSGIGRFRVNAFLQQSHVGLV
                     LRTIPALIPTIDSLGLPRVLKDVALSKRGLIIMVGATGSGKTTSQAAMIDWRNENSFG
                     HIITVEDPVEFVHPHKNCIVTQREIGIDTDSWELALKNTLRQAPDVILMGEIRDRETM
                     DLAIAFAETGHLCMATLHANSSNQALDRMINFFPEERRTQLLMDLSLNMKALVSQRLL
                     PRQEGKGRVAAVEILLNTPLVADLIFQGKVGDLKDLMKRSRELGMQTFDQALFDMYEA
                     NLITYEDALRNADSVNDLRLQIKLESERSRSTDLAAGTEHMTFG"
     gene            2031..2954
                     /locus_tag="VIN75_21390"
     CDS             2031..2954
                     /locus_tag="VIN75_21390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8976784.1"
                     /translation="MLRSSERIEPMTKQYEATPSRKVAFLGLGVMGGPMAGHLAAAGH
                     QVTVYNRTAAKAQAWVGRHGGKAAATPRAAAEGADFVFSCVGNDADLRSVVLGDDGAF
                     AGMARGAVFIDNTTASADVARELHGLAGARGLHFIDAPVSGGEAGAINGLLTVMCGGD
                     AEPFDAMKPVAMAYAKAVTRLGDSGSGQLAKMVNQICIAGIVQGLSEAVAFGTRAGLD
                     MPAVLDVIGKGAAQSWQMDNRGKTMVEDKFDFGFAVDWMRKDLGLVLDEARDNGARLP
                     LTALVDQFYAEVQNRGGGRWDTSSLIRLLNK"
     gene            complement(3141..3773)
                     /locus_tag="VIN75_21395"
     CDS             complement(3141..3773)
                     /locus_tag="VIN75_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8976785.1"
                     /translation="MHTLSTLRRRHPLATTLVAGALAAATLASMQGCIALLGGAAVAG
                     GLSLNDRRTGGTQIEDQSIELKSGGRLREAIGEKGHINVTSYDRIVLLSGEVPTEADK
                     AAAEKAVRDIEGVSNVVNELEVGPDSTLSTRSSDTVITTRVKSALIDAKDVQASAVKV
                     VTERGNVYLMGRVTEREATRAAEIARAQPSVLKVVRVFEILTEEQLSNLR"
     gene            complement(3781..4389)
                     /locus_tag="VIN75_21400"
     CDS             complement(3781..4389)
                     /locus_tag="VIN75_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8976786.1"
                     /translation="MLEPRIQQHFFESADLSYQAAEAMARPIGDAAQAIVDCITSGGR
                     VWVHGRGVAQAPAQLLGAALSGRFERERPGLAAMVLAVDPLLLSTPPETLADAPAVTQ
                     LRVLGQPGDVLVAFALRPDDSLLASVVEAAHDREMSAIVVAGREPGSTTVRGNAQMTM
                     AETDIWIGVPHERAARICELQLLTLHALCDAVDLQLLGDADL"
BASE COUNT          737 a         1606 c         1665 g          715 t
ORIGIN      
        1 gttccaggag cagcaccagg acacgcagct catggtcagc tgggacgcgc gcagcctcga
       61 cctgcacacc aagccctacg atccggcgct gggcacgctc gcgctggtca agctgttcgg
      121 ccaggatccg cagctgtcgc agctcgggcc cgaggcgctg gcggtgttcg gcaactacct
      181 cgactacgtg caggtcgagg ccggccgcca ggtcatcggg caggacgagc agggcgactt
      241 cctgctgatc gtgctggacg gcaccgtcgc cgtcgagcgc gtgcagccct ggggcgcgaa
      301 ggcgcgcctg ggcgaatcgc gcgcgggcga cgtgctcggc gagatgtcgc tgctcgacgc
      361 cggcgcccgc ttctccgcct gcaccacgct cacgccggcc aagttcgccg tgctcggcgc
      421 ggccgcgctc gaccgcatga tgcagcacga gccgcggctg gccgcggcga tgctcgcctg
      481 gctcgcgcgc cggctgtcgc tccggctgcg ccaggtcagc gcccggctgt ccgccctgct
      541 cacgcgcgaa taacggcgat ttgccacgct ttgcgggggc gaatggcgcg ttgccattga
      601 acacccccgt cgcccgccga tgctggcaag atcgcggatg tagtcacgaa gaggttgcga
      661 agccatggaa agagaacaag catcgaagtt cgtcaatgac ctgctgcgcc tgatggtcac
      721 gcgcaacggc tccgacctgt tcctcacggc cgatttcccg ccggccatca aggtcgacgg
      781 caaggtcacc aaggtgtcgc cgcagccgct caccggcgcc cacacgatgg cgttggcgcg
      841 cgcgatcatg aacgacaagc aggcgtcgga cttcgagcgc accaaggagt gcaacttcgc
      901 gatctcgccg tccggcatcg ggcgcttccg cgtcaacgcc ttcctgcagc agagccatgt
      961 cggcctcgtg ctgcggacca tcccggccct gatccccacc atcgacagcc tggggctgcc
     1021 gcgcgtgctc aaggacgtcg cgctgagcaa gcgcggcctg atcatcatgg tgggcgccac
     1081 cggctcgggc aagacgacct cgcaggcggc gatgatcgac tggcgcaacg agaactcgtt
     1141 cggccacatc atcacggtcg aggatccggt cgagttcgtg cacccgcaca agaactgcat
     1201 cgtcacgcag cgcgagatcg gcatcgacac cgacagctgg gagctggcgc tgaagaacac
     1261 gctgcgccag gcgcccgacg tcatcctgat gggcgagatc cgcgaccgcg agaccatgga
     1321 cctggccatc gccttcgccg agaccggcca cctgtgcatg gccacgctgc acgcgaacag
     1381 ctccaaccag gcgctggatc gcatgatcaa cttcttcccc gaagagcgcc gcacgcaact
     1441 gctgatggac ctgtcgctga acatgaaggc gctggtctcg cagcgcctgc tgccgcgcca
     1501 ggaaggcaag ggccgcgtcg ccgcggtcga gatcctgctg aacacgccgc tggtggccga
     1561 cctcatcttc cagggcaagg tcggcgacct gaaggacctg atgaagcgct cgcgcgagct
     1621 gggcatgcag accttcgacc aggccctgtt cgacatgtac gaggccaacc tcatcaccta
     1681 cgaggacgcg ctgcgcaacg ccgactccgt caacgacctg cggctgcaga tcaagctcga
     1741 gagcgaacgc tcgcggtcga cggacctggc agccggcacc gagcacatga cgttcggctg
     1801 aacgagcgcg cgacgccttg cgagggtgtc aggtcttgaa agttgcgcgg cagggccttg
     1861 gcatcggggt caggtcttga aagttgcgcg aggtccggtc gctctttggc ggaaattcgg
     1921 tcgcgcaact ttcaagacct gaccccgtcg cgcggtgctc aacgcgcaac tttcaagacc
     1981 tgacacccgg ccctcgacag cgacaccgcc acggtgatcg gattcctcgt atccttcgat
     2041 cctccgaacg gatcgaaccg atgaccaaac aatacgaagc cacgccgtcc cgcaaggtcg
     2101 ccttcctcgg gctgggcgtg atgggcggcc cgatggccgg ccacctggcc gccgcggggc
     2161 accaggtgac cgtctacaac cgcacggcgg ccaaggcgca agcctgggtc ggccggcacg
     2221 gcggcaaggc cgcggccacg ccgcgggcgg cggccgaggg cgccgacttc gtgttctcgt
     2281 gcgtgggcaa cgacgccgac ctgcgctcgg tcgtcctggg tgacgacggc gccttcgccg
     2341 gcatggcgcg cggcgccgtc ttcatcgaca acaccaccgc atcggccgac gtcgcgcgcg
     2401 agctgcacgg cctggccggg gcgcgcggcc tgcacttcat cgacgcgccg gtctccggcg
     2461 gcgaggcggg cgccatcaat ggcctgctga cggtgatgtg cggcggcgac gccgagccct
     2521 tcgacgcgat gaagccggtg gcgatggcct acgcgaaggc ggtcacgcgg ctgggcgaca
     2581 gcggctcggg acagctggcc aagatggtca accagatctg catcgcgggc atcgtgcagg
     2641 gcttgtccga ggccgtggcc ttcggcacgc gcgccgggct ggacatgccg gccgtcctcg
     2701 atgtcatcgg caagggcgca gcccagagct ggcagatgga caaccgcggc aagacgatgg
     2761 tggaggacaa gttcgacttc ggcttcgccg tcgactggat gcgcaaggac ctcggcctgg
     2821 tgctggacga ggcgcgcgac aacggcgccc gcctgccgct gacggcgctc gtcgaccagt
     2881 tctatgccga ggtgcagaac cgcggcggcg ggcgatggga cacgtcgagc ctgatccggc
     2941 ttctgaacaa atagccggcc gctcgctcat gcaaagcgga cgccgcggcg tccgttttca
     3001 ttccatgtct tcagccggct tccgagccgt catcctgcgc gcaggatgac gcgtcttggc
     3061 gcgctgcggc gcggtcatcg cggcgcgaat gcacggacgc tcgaggcgcg atgcgccctg
     3121 cgcgcgtcaa gcactggcgc tcagcgcagg ttgctgagct gttcttcggt caggatctcg
     3181 aacacgcgta ccaccttgag cacgctgggc tgggcgcggg cgatctcggc tgcgcgcgtg
     3241 gcttcgcgtt cggtgacgcg gcccatcagg tagacgttgc cgcgctcggt gaccaccttg
     3301 acggcgctgg cctgcacgtc cttggcgtcg atcagcgcgc tcttgacgcg cgtggtgatc
     3361 acggtgtcgc tggagcgcgt gctgagcgtg ctgtcggggc cgacctccag ttcgttgacg
     3421 acgttgctga cgccctcgat gtcgcgcacg gccttctcgg cggccgcctt gtcggcctcg
     3481 gtgggcacct cgccggacag cagcacgatg cggtcgtagc tggtgacgtt gatgtggccc
     3541 ttctcgccga tcgcctcgcg caggcgtccg ccggacttca gctcgatgga ctggtcctcg
     3601 atctgcgtgc cgcccgtgcg gcggtcgttg agcgacaggc cgccagcgac ggctgcgccg
     3661 cccagcagcg cgatgcagcc ctgcatcgag gccagcgtgg cggcggcgag ggcgccggcg
     3721 acgagggtgg tggccagcgg gtggcggcgg cggagggtgg acagggtgtg catggtgggt
     3781 tcagaggtcg gcgtcgccga gcagttgaag gtcgacggca tcgcacaggg cgtgcagcgt
     3841 caggagctgg agttcacaga tgcgcgcggc gcgctcgtgc ggcacgccga tccagatgtc
     3901 ggtctcggcc atcgtcatct gcgcgttgcc gcgcacggtg gtcgaccccg gctcgcggcc
     3961 ggcgacgacg atggcgctca tctcgcggtc atgtgccgcc tcgacgaccg aggccagcag
     4021 gctgtcgtcg ggccgcagcg cgaatgcgac gagcacgtcg cccggctggc ccagcacgcg
     4081 cagctgcgtg acggcggggg cgtcggcgag cgtctcgggc ggggtggaca gcagcagcgg
     4141 gtcgacggcc agcaccatgg cggccaggcc cgggcgttcg cgctcgaagc ggcccgacag
     4201 cgcggcgccc agcagctgcg ccggggcctg cgcgacgccg cgcccgtgca cccagacgcg
     4261 gccgcccgag gtgatgcagt cgacgatcgc ctgggcggcg tcgccgatgg gtcgcgccat
     4321 ggcctcggcc gcctggtagc tcaggtcggc gctttcgaag aagtgctgtt ggatgcgggg
     4381 ttcgagcatg gatgccaggt caggcggcga cggggccgcg cggggatcat agttcagcgc
     4441 gccgtaggac agagtcgcgt tcgcgccgtc gagttccgtc gggcctggaa ggccacccat
     4501 tgacgatttc cgtcgccacg aaggcggcgg gtcgccgttt gttgacgtgc cagcagcaat
     4561 cggacgaagg aatcgcacga gtatagggtt tgcccctatc aagggaaacg acggccggcc
     4621 gatgggccgc tcatccctcg tcgaaggccg cccgcagcca ttgcacgcgg tcgccgtcca
     4681 ccgcgacgac gtcgaagcgg cacggcggga cacgcggcag cgc
//
LOCUS       DATCRO010000785         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_931425, whole genome shotgun sequence.
ACCESSION   DATCRO010000785 DATCRO010000000
VERSION     DATCRO010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931425"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VIN75_21405"
     CDS             complement(<1..231)
                     /locus_tag="VIN75_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976787.1"
                     /translation="MSATACVLALAAGAALAADALPNRNCADGVCMANVDPAQAIEPC
                     GNASIVIAWAQAGGAMAIECDLGDAAADRPLYV"
     gene            327..1172
                     /locus_tag="VIN75_21410"
     CDS             327..1172
                     /locus_tag="VIN75_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8976788.1"
                     /translation="MRIEIEPGVRLYVDVEGTQFVPDGPALREKPALVLLHGGPGFDH
                     SAFRPFFGRVADIAQVIYVDHRGHGRSDRRPPEEWTLDTFADDVVRLCDALGIVKPIV
                     LGQSFGGFVAQRYIDRHPAHPSKVILSSTSPHMKQERKEAMFAKLGGEAARDSCRRFW
                     SDPNPDTWADYGVHCRHLYNTTPQDPDAGARTIHEFDILYRSAAGEQRTMDLLPGLAK
                     AQCPVLVMVGSQDPVCPVDDARDIAAALPPQWMQYREFPNVGHGAWRDDADAAFEVLR
                     RFILE"
     gene            1175..1603
                     /locus_tag="VIN75_21415"
     CDS             1175..1603
                     /locus_tag="VIN75_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976789.1"
                     /translation="MRHRLFLASTLLLAGCAPVLHPHGERTQPEVDGQLLSAGVPRAG
                     VRISACQGVPEGGVELSSHRCDGIAWATTDAQGRFHFEPIERRKLMLDHHTAAYVILT
                     VESTGRELTWHRDYPGEAPVHESLRCELGDRLACETAQVP"
     gene            1641..2069
                     /locus_tag="VIN75_21420"
     CDS             1641..2069
                     /locus_tag="VIN75_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976790.1"
                     /translation="MNAIHEEVHATAERAVAALQERAGGRLDWSVASLEVVDEMLVEV
                     SEYVADLDEAVVTNLVQQLGCYVLEVARRTYGGAYFWHQDGEQPVLVVGEPDAHVALM
                     TWSKVTGRITGDAGDDIVFFFDGFAARAKQPEPGSDVLFL"
     gene            2273..2467
                     /locus_tag="VIN75_21425"
     CDS             2273..2467
                     /locus_tag="VIN75_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY8976791.1"
                     /translation="MNKDQVNGRAETLKGDVKEAAGKVVGNERLTAEGKADQAAGKVQ
                     SKVGDVKNDVGNAVKNAIDR"
     gene            complement(2620..3003)
                     /locus_tag="VIN75_21430"
     CDS             complement(2620..3003)
                     /locus_tag="VIN75_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEY8976792.1"
                     /translation="MPLVTLTVRQGKDAAFKSAVLEGVHRALVASGVPDADRFHRVLE
                     LSAQDFRFDPSYPDLTRPRTDDFVLIEVLLSAGRSVKIKRKIAADLIAALRESPGLDP
                     ENVMLAFKETSWENWSFGGGRFIHV"
     gene            complement(3016..3246)
                     /locus_tag="VIN75_21435"
     CDS             complement(3016..3246)
                     /locus_tag="VIN75_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY8976793.1"
                     /translation="MKEFLVEGMSCGHCISAVTEAIHKLDPAADVNVDLGSKHVRVHS
                     DVDRFLLSQALVDAGYDPVPVGLEGPGSPDNV"
BASE COUNT          529 a         1194 c         1212 g          495 t
ORIGIN      
        1 cacgtacagc ggccggtccg cggcggcgtc gccgaggtcg cactcgatgg ccatcgcgcc
       61 gccggcctgg gcccacgcga tcacgatgct cgcattgccg cagggctcga tcgcctgcgc
      121 ggggtcgacg ttggccatgc acacgccgtc ggcgcagttg cgattgggca gggcatcggc
      181 ggcgagggcg gcgccggccg ccagggccag gacgcatgcg gtggcggaca cgcgaaatgc
      241 ggacgtcggg cggtggcgga ccatcgccgc atgttacgtc ccggctatgc tcgcggcttc
      301 gcccccgccc gccaaggact tcacgtatgc gcatcgagat cgaacccggc gtccgcctct
      361 acgtcgacgt ggaaggcacg caattcgtgc ccgacggccc ggcgttgcgc gagaagcctg
      421 cgctggtgct gctgcacggc ggccccggct tcgaccactc ggccttccgg ccgttcttcg
      481 gccgcgtggc cgacatcgcg caggtgatct acgtcgacca ccgcggccac ggccgcagcg
      541 accgccggcc gcccgaggag tggacgctgg acaccttcgc cgacgacgtc gtgcgcctgt
      601 gcgacgcgct gggcatcgtc aagccgatcg tgctggggca gtcgttcggc ggcttcgtgg
      661 cgcagcgcta catcgaccgc catccggcgc atccgtccaa ggtgatcctg tcgtcgacgt
      721 cgccgcacat gaagcaggag cgcaaggagg ccatgttcgc gaagttgggc ggcgaggccg
      781 cgcgcgactc gtgccggcgc ttctggagcg atccgaaccc cgacacctgg gccgactacg
      841 gcgtgcactg ccgccacctg tacaacacca cgccgcagga tccggacgcc ggcgcgcgca
      901 cgatccacga gttcgacatc ctgtaccgct cggccgcggg cgagcagcgc accatggacc
      961 tcctgcccgg cctcgcgaag gcgcagtgcc ccgtgctggt gatggtgggc agccaggatc
     1021 cggtgtgccc cgtcgacgac gcgcgcgaca tcgccgcggc cctgccgcca caatggatgc
     1081 agtaccgcga gttcccgaac gtgggccacg gcgcctggcg cgacgacgcc gatgcggcgt
     1141 tcgaggtcct gcgtcgtttc atcctggagt gaagatgcgc caccgcctgt tccttgcctc
     1201 gaccctcttg ctggccggct gcgcgccggt gctccatccg cacggcgagc gcacgcaacc
     1261 cgaggtcgac ggccagctcc tgagcgcggg cgtgccgcgc gccggcgtgc gcatctcggc
     1321 ctgccagggc gtgcccgagg gcggcgtcga gctgtcgtcg catcgctgcg acggcatcgc
     1381 gtgggccacc accgacgcgc agggccgctt ccatttcgag ccgatcgaac gcaggaagct
     1441 gatgctggac caccacaccg cggcctacgt gatcctcacc gtcgaatcga ccggccgcga
     1501 gctgacgtgg catcgcgact acccgggcga ggcgcccgtc cacgagtcgc tgcgctgcga
     1561 gctgggcgac aggctcgcgt gcgagacggc gcaagtcccc tgagccggcg cgctgccgcc
     1621 cgctgcagaa tgccgctccc atgaacgcca tccacgaaga agtccacgcc accgccgagc
     1681 gcgcggtcgc cgcgctgcag gagcgcgcag gcgggcggct cgactggtcg gtcgcgagcc
     1741 tggaggtggt cgacgagatg ctggtcgagg tgtcggagta cgtcgccgac ctcgacgagg
     1801 cggtcgtcac caacctcgtg cagcagctcg gctgctacgt cctggaagtg gcgcggcgga
     1861 catacggcgg cgcgtatttc tggcaccagg acggcgagca gcccgtcctc gtggtcggcg
     1921 agcccgacgc ccacgtcgcg ctgatgacct ggagcaaggt cacgggccgc atcacaggcg
     1981 acgcgggcga cgacatcgtg ttcttcttcg acggattcgc cgccagggcc aagcagcccg
     2041 agcccggcag cgacgtcctg ttcctctgag agtccgccag ctcgacaggg ccgcgcgcgt
     2101 cgtcgtttgt cctacacgac gatcgaaccc gctccgacac gacgagcgac tccggtccga
     2161 cacgaccgcg cgcgccgcgg ccgcatagtt ggatccatgc cgaacgacat cggccgcaga
     2221 actcgaacaa cagccgatgt cgaccggccc cacactttca aggagcacga tcatgaacaa
     2281 ggaccaagtc aacggccgcg ccgagaccct caagggcgac gtcaaggagg ccgccggcaa
     2341 ggtggtcggc aacgagcgcc tgaccgccga aggcaaggcc gatcaggccg ccggcaaggt
     2401 gcaatcgaag gtcggcgacg tcaagaacga cgtcggcaac gccgtcaaga acgcgatcga
     2461 ccgctgatcg atggccggcg cccgcgcccc agtgccggcg cgggcgccgg cagcccggtt
     2521 cccgcgatcg attcgtcggt cgcatcgact ctcgaacgga cgatgacgtc cggacatcca
     2581 gtgcatgtcc gggcgtcgtc gtttcggaag ccgccggccc tagacgtgga tgaagcggcc
     2641 gccgccgaac gaccagttct cccagctcgt ctccttgaac gcgagcatca cgttctcggg
     2701 gtcgagcccg ggcgactcgc gcagcgcggc gatcaggtcg gcggcgatct tgcgcttgat
     2761 cttgacgctg cgcccggccg acagcagcac ctcgatcagc acgaagtcgt cggtgcgcgg
     2821 ccgggtgagg tccgggtagc tcgggtcgaa gcggaagtcc tgcgccgaca gttcgagcac
     2881 gcggtggaag cgatcggcgt cgggcacgcc ggaggcgacg agcgcgcggt gcacgccttc
     2941 gaggacggcc gacttgaagg cggcgtcctt gccttgacgc acggtgaggg tgaccagggg
     3001 catgggttct ccgtatcaga cgttgtcggg cgagccgggg ccttcgaggc cgaccggcac
     3061 ggggtcgtag ccggcgtcga cgagcgcctg cgacagcagg aagcggtcga cgtccgagtg
     3121 cacgcgcacg tgcttgctgc cgaggtcgac gttcacgtcg gcggccggat cgagcttgtg
     3181 gatggcttcg gtgacggcgc tgatgcagtg tccgcagctc atgccttcga cgaggaattc
     3241 cttcatggcg ttctcctgag ggggcaggtg gacagtgtcg cccggtgcgc cggatcggtg
     3301 gtgggcacgg ccgggcggcc ccgggccgcg cgggggattg cggatgcgcg cggcgccccg
     3361 acatgaccct tcgcgtgggg ctttttccaa tctgcgcaga tcgttacagg cagaccgtcg
     3421 acactggcgg
//
LOCUS       DATCRO010000786         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_605163, whole genome shotgun sequence.
ACCESSION   DATCRO010000786 DATCRO010000000
VERSION     DATCRO010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605163"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIN75_21440"
     CDS             complement(<1..468)
                     /locus_tag="VIN75_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8976794.1"
                     /translation="MTTALDNLLDFDQSALKAYCERVGEKSFRATQLFRWIHHRGVGN
                     FDDMTDLARSLRAKLAGVAEVRPLAAITDKTSADGTRKWLFDVGQGNAVETVYIPEDD
                     RATLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEIIAQLWHAEHQMRAKLGLGA"
     gene            complement(522..944)
                     /gene="ndk"
                     /locus_tag="VIN75_21445"
     CDS             complement(522..944)
                     /gene="ndk"
                     /locus_tag="VIN75_21445"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8976795.1"
                     /translation="MTERTLSIIKPDAVAKNVVGQILARFEGAGLKIVAARMMQLSQA
                     DAEAFYAVHKARPFFGDLVKFMISGPVMVQVLEGEGAILKNRDLMGATDPKKAEKGTI
                     RADFADSIDANAVHGSDAPETAANEIAFFFPGMQVHSR"
     gene            complement(1071..>2974)
                     /locus_tag="VIN75_21450"
     CDS             complement(1071..>2974)
                     /locus_tag="VIN75_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8976796.1"
                     /translation="PAPAAEAAPPAEMLEVREPRPPRARKTPASAAAVEATAPAAEAV
                     EHVADDAGRQAAQGDDGGDADASSQGGEGGEAGEGREGRRSRNRRRRRGRRGGGGDDA
                     TGARAEGAEGDDEGDADGEPGEEGGEGRPPRGERGPRPERGNRPIVEFSTADVSDTFA
                     GVLSGDYDNEPELAEGESAIPAKRVLAAEPDAPKLHKVLAQSGIGSRRDMEQLITDGK
                     VTVNGEVAHTGQRISFGDEVRVAGRVVKLRIAPPPARVIAYHKPAGEVVTMDDPEKRP
                     TVFRRLPRLAHGKWQSVGRLDINTEGLLLFTNSGDLANKLMHPRFGVEREYAVRVLGT
                     LDQESRDKLLAGVSIDGQEAHFKSIEDGGGEGANRWYRVVITEGRNREVRKLFDAVGM
                     AVSRLIRIRYGSVVLPKGLRRSVWVDLDENDVRRLRQLAGADNGGGQPRGDKPPREDG
                     GQQQQPRGDKPQRGDRGDRNDRGPRGDNGQRANNGGGQQQQPKQRDRDRGRNRDRGGR
                     QDQSPAPFREAGDPAYIPNPLQQTFDRRAIQQRERPVIVDYDDENDGPIGPIPNPLQQ
                     TYDKRGVSGNANAFMKGGKGKRGGGGGGGGEKQPDPLKTAVGYIGADTFIKKLNNRGG
                     GGGRGRGRR"
BASE COUNT          350 a         1032 c         1094 g          498 t
ORIGIN      
        1 ggcgcccagg ccgagcttgg cgcgcatctg gtgctcggca tgccacagct gggcgatgat
       61 ctcgccggtc gtgaggttgc ggctgaagcc ctggtgcccg gtcgagcaga agcggcagcc
      121 gacggcgcat ccggcctgcg aggagatgca cagcgtggcg cgatcgtcct cggggatgta
      181 gaccgtctcg acggcgttgc cctggccgac gtcgaacagc cacttgcgcg tgccgtcggc
      241 cgaggtcttg tcggtgatcg cggccagggg gcgcacctcg gcgacgccgg ccagcttcgc
      301 gcgcagcgag cgcgccaggt cggtcatgtc gtcgaagttg cccacgccgc gatggtggat
      361 ccagcggaac agctgggtcg cgcggaacga cttctcaccc acgcgctcgc aatacgcctt
      421 gagggcggat tgatcgaagt cgagcaggtt gtccagagcg gtcgtcatcg cgaggtttcc
      481 aggggacacg gctcggggcg agccgtgccg caggtcgcga attaacgcga gtggacttgc
      541 atgccgggga agaagaacgc gatttcgttc gcggccgtct ccggggcgtc cgagccgtgc
      601 acggcgttgg cgtcgatcga gtcggcgaag tcggcgcgga tcgtgccctt ctcggccttc
      661 ttcgggtcgg tggcgcccat caggtcgcgg ttcttgagga tggcgccttc gccttcgagc
      721 acttgcacca tcaccgggcc ggagatcatg aacttgacca ggtcgccgaa gaacgggcgg
      781 gccttgtgca ccgcgtagaa cgcctcggcg tcggcctgcg acagctgcat catgcgggcg
      841 gcgacgatct tcaggccggc gccttcgaag cgggccagga tctggccgac gacgttcttg
      901 gcgacggcgt caggcttgat gatcgagagg gtacgttcgg tcattttgat tctccagagg
      961 ggtgaaactg agcaaagccc gtcattttag accgggcgtg tgaatttgat aagcgcatcg
     1021 gggccaagga accgcgtcac cgggatgaga accgattccc gtcccgggat tcaacgccgg
     1081 ccgcgcccgc ggccaccgcc gccgccgcga ttgttgagct tcttgatgaa ggtgtccgcg
     1141 ccgatgtacc cgacggccgt cttgagcggg tcgggctgct tctcgccgcc gcctccgccg
     1201 ccaccgccgc gcttgccctt gccgcccttc atgaaggcgt tggcgttgcc ggacacgccg
     1261 cgcttgtcgt aggtctgctg cagcgggttc gggatcgggc cgatcgggcc gtcgttctcg
     1321 tcgtcgtagt cgacgatgac gggacgctcg cgctgctgga tcgcgcgacg gtcgaaggtc
     1381 tgctgcagcg ggttggggat gtaggccgga tcgcccgcct cgcggaacgg cgcggggctc
     1441 tggtcctggc ggccaccgcg gtcgcggttg cggccacgat cgcggtcgcg ctgcttgggc
     1501 tgttgctgct ggccgccgcc gttgttggcg cgctggccgt tgtcgccgcg cgggccgcgg
     1561 tcgttgcggt cgccccgatc gccgcgctgc ggcttgtcgc cacgcggctg ttgctgctgg
     1621 ccgccgtcct cgcgcggcgg cttgtcgccg cgcggctgcc cgccgccgtt gtcggcgccc
     1681 gcgagctggc gcaggcgtcg cacgtcgttc tcgtcgagat cgacccacac cgagcggcgc
     1741 aggcccttgg gcagcaccac cgagccgtag cggatgcgga tcaggcggct cacggccatg
     1801 cccaccgcgt cgaacagctt gcgcacctcg cggttgcggc cttcggtgat gacgacgcgg
     1861 taccagcggt tggcgccttc gccgccgccg tcctcgatcg acttgaagtg cgcctcctgg
     1921 ccgtcgatgg acacaccggc cagcagcttg tcgcgcgact cctggtcgag cgtgcccagc
     1981 acgcgcacgg cgtactcgcg ctcgacgccg aagcgcgggt gcatcagctt gttggccagg
     2041 tcgccggagt tcgtgaacag cagcaggcct tcggtgttga tgtccaggcg cccgaccgac
     2101 tgccacttgc cgtgcgccag ccgcggcagg cggcggaaca cggtcgggcg cttctcgggg
     2161 tcgtccatcg tgacgacctc gccggcgggc ttgtggtacg cgatcacgcg tgccggcggc
     2221 ggcgcgatgc gcagcttgac cacgcggccc gcgacgcgca cctcgtcgcc gaacgagatg
     2281 cgctggcccg tgtgggcgac ctcgccgttg acggtgacct tgccgtcggt gatcagctgc
     2341 tccatgtcgc ggcgcgagcc gatgcccgac tgcgcgagca ccttgtgcag cttgggcgcg
     2401 tcgggctcgg cggccagcac gcgcttggcc gggatggcgc tctcgccctc ggccagctcg
     2461 ggttcgttgt cgtagtcgcc cgacagcacg ccggcgaacg tgtcggacac gtcggcggtg
     2521 gagaactcga cgatcggacg attgccgcgc tccggacgcg gaccgcgctc gccgcgcggg
     2581 ggacggccct cgccgccctc ctcgccgggc tcgccgtcgg cgtcgccttc gtcatcgccc
     2641 tcggcgcctt ccgcgcgcgc gccggtcgcg tcgtcgccac caccgccacg gcggccgcga
     2701 cgacggcggc ggttgcgcga gcggcggcct tcgcggccct cgcccgcctc gccgccttcg
     2761 ccgccctggg acgacgcgtc ggcgtcgccg ccgtcgtcac cctgcgcggc ctggcggccg
     2821 gcgtcgtcgg cgacgtgctc gacggcttcg gcggccggcg ccgtcgcttc gaccgcggcg
     2881 gcggacgcgg gcgtcttgcg ggcgcgcggg ggacgcggct cgcgcacttc cagcatctcg
     2941 gccggcggcg cggcttcggc ggccggtgcg ggcg
//
LOCUS       DATCRO010000787         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_796790, whole genome shotgun sequence.
ACCESSION   DATCRO010000787 DATCRO010000000
VERSION     DATCRO010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796790"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VIN75_21455"
     CDS             <1..568
                     /locus_tag="VIN75_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY8976797.1"
                     /translation="LPQLFDGRRQLMVQHFMLGPGWEQGCKSCSFMADHLLGAQIHLQ
                     HRDVSVLLVSRAPLADIERFRRRMGWPFKWASASGNDFNLDFHVSFPEGTRIGGEVYY
                     NWHMTPFPKEEAPGISFFCKDDAGQVFHTYSTFGRGVEVMMGTYHLLDLAPKGRDEER
                     DDHGMAWVRHHDRYEDAPASSSCCASNA"
     gene            597..1301
                     /locus_tag="VIN75_21460"
     CDS             597..1301
                     /locus_tag="VIN75_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976798.1"
                     /translation="MAGIGPWLAVAGVGALHGLNPATGWGLVAAWGVRAGDRARAWRA
                     LAPVALGHAGAVALVAALAALGMAAGRGLLPWLAGGGAVFAAAVHVAGHVDGHARRLA
                     GSTGLALWSLAVGSIHGAGMMLVPALVPLCVSTGPAREITASGSIVPALAVVALHLAA
                     MLAVTAALAGLVGRGWIALRGRPHRRPCAAQEAPLAAEAAGAAGTRRASTSQGSRRWP
                     KRTVASKGVMSAGMAK"
     gene            complement(1286..2044)
                     /locus_tag="VIN75_21465"
                     /pseudo
     CDS             complement(1286..2044)
                     /locus_tag="VIN75_21465"
                     /inference="COORDINATES: protein motif:HMM:NF027432.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
     gene            complement(2041..>2506)
                     /locus_tag="VIN75_21470"
     CDS             complement(2041..>2506)
                     /locus_tag="VIN75_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976799.1"
                     /translation="GTAGGMYWLYFGERPENFFPAAAVLLALPLLWDQSRFAGFAATW
                     RIAGMLVLFLPMLVLANWGTLSYLHLGHDVVQGLYQVLGFAGTAVLVWWGARRDWRDV
                     TGTGVTLFIVFLYTKLFDWWWDWMPKYVFFLVMGLGAVLLLFVLRRVRGGTA"
BASE COUNT          342 a          890 c          940 g          334 t
ORIGIN      
        1 gctgccgcag ctgttcgacg gccgccgcca gctgatggtg cagcacttca tgctcgggcc
       61 gggctgggag cagggctgca agagctgctc gttcatggcc gaccacctgc tcggcgcgca
      121 gatccacctg cagcaccgcg acgtcagcgt gctgctcgtg tcgcgcgcgc cgctggccga
      181 tatcgagcgc ttccgccggc gcatgggctg gcccttcaag tgggcgtcgg ccagcggcaa
      241 cgacttcaac ctcgacttcc acgtcagctt ccccgaaggg acgcgcatcg gcggcgaggt
      301 ctactacaac tggcacatga cgccgtttcc caaggaggag gcgccaggca tcagcttctt
      361 ctgcaaggac gacgcggggc aggtcttcca cacctattcg accttcggcc gcggcgtgga
      421 agtgatgatg ggcacgtacc acctgctgga cctggcaccc aagggccgcg acgaggagcg
      481 cgacgaccac ggcatggcct gggtgcgcca tcacgaccgc tacgaggacg cgccggcgtc
      541 ctcatcgtgc tgcgcctcga acgcttgacg cgacgcggtc gcagggcgga ggcgtcatgg
      601 cgggcatcgg tccctggctg gcggtggcgg gcgtcggcgc gctgcacggc ctgaatcccg
      661 cgacgggctg gggcctggtc gcggcctggg gcgtgcgtgc gggcgaccgc gcccgcgcat
      721 ggcgtgcctt ggcgcccgtc gcgctgggcc acgccggtgc ggtcgcgctc gtcgccgcgc
      781 tggcggcact gggcatggcc gccggccgcg gcctgctgcc ctggctcgcc ggcggcggcg
      841 cggtgttcgc ggcggcggtg catgtggcgg gccacgttga cggacacgcc cgtcgcctcg
      901 ccggatcgac cgggctggcg ctgtggtcgc tcgccgtcgg ctcgatccac ggcgccggca
      961 tgatgctggt gccggcgctg gtgccgttgt gcgtgtcgac cgggccggca cgggagatca
     1021 cggcctcggg ctcgatcgtg ccggcgctgg cggtcgtcgc gctgcacctg gccgcgatgc
     1081 tggccgtgac ggcggcgctg gccgggcttg tcggccgcgg atggatcgcg ttgcgcggcc
     1141 gaccgcatcg acggccgtgc gcggctcagg aggcgccgct cgcggccgag gcggcgggcg
     1201 cggccggaac gcggcgggcg tcgacgagcc agggctccag gcgctggccg aagcgcacgg
     1261 tggcctcgaa gggcgtcatg tcggccggca tggcgaagta gttcgatgac aggccttcga
     1321 agacggcccg gcagcgcagc ggcacgtcga tctgctcgcc cggcagggcc tcgatccgcc
     1381 cgccgaccgt gtcgccgcgt cggccgccgg cccgcagctc gacctggcca tggacgatcg
     1441 catacatcga gcgatcgggg tagcgggcgc gcaaggcgcc gcggtccagg ccggcatcga
     1501 cggcgaacag ccggctgccg acctcgcggc cccattgcgc cggcgtgggc cagcgcgggc
     1561 cggtggcggg acgctgcggc tgcgacgcgg cggcctcggc caaggccacg cggcgcaagg
     1621 attcccggta tgccggtccg tccagctcca gcacgagata gacctcgcgg gcgagctggc
     1681 gatggcccca cgagtcctcg ttgaaggccc ggccgcccgc gacgtcggcg acgtcgaagc
     1741 cgagctcgcg cagcttcgcg ggcgtcagcc attcggcgtg gccgccgccc tggttgtagg
     1801 tgtcgtagcc gccgtcgtgg acgtcctgcg gggccagtgt gcgccagcgc agctgcagcg
     1861 acaggccgct gttctcgttg ccggcgaccg gaacgttggg cagggtgagc tcgcgctggc
     1921 tcaggcgcag cgtgccctcg ccggcgccgc tgcggttcca tgccgcgccg ccgagcgcca
     1981 cggcgttggt caggatgatc agcgccgcgc cgaccgcggg aacgagacgt cgggcgcgca
     2041 tcatgccgtt cccccgcgca cgcggcgcag cacgaacagc agcagcacgg cgcccaggcc
     2101 catcaccagg aagaacacgt acttgggcat ccagtcccac caccagtcga acagcttggt
     2161 gtagagaaag acgatgaaca gcgtgacccc cgtgccggtc acgtcgcgcc agtcgcggcg
     2221 cgcgccccac cagacgagga cggccgtgcc ggcgaagccc agcacctggt acagcccctg
     2281 gaccacgtcg tgcccgaggt gcaggtagct gagcgtgccc cagttggcga gcacgagcat
     2341 cggcaggaac agcaccagca tgccggcgat gcgccaggtg gcggcaaagc ccgcgaaccg
     2401 cgactggtcc cacagcagcg gcagcgcaag cagcaccgcg gccgccggga agaagttctc
     2461 ggggcgctct ccgaagtaga gccagtacat gccgccggcg gtgccc
//
LOCUS       DATCRO010000788         7065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_583014, whole genome shotgun sequence.
ACCESSION   DATCRO010000788 DATCRO010000000
VERSION     DATCRO010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583014"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VIN75_21475"
     CDS             complement(<1..1668)
                     /locus_tag="VIN75_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8976800.1"
                     /translation="MALLTLSNAHLAFGHVALLDGAEFALETAERVALIGRNGTGKSS
                     LLKILAGIDKPDDGLLQVERGLRRTYVPQEPLFAPGVSVFDAVSEGVREAREAREKFE
                     NHDGVDHDELDRLQTRLEALGGWDWERRVDETLHRLDLEPQQQVDKLSGGLRKRVALA
                     QALVAAPDVLLLDEPTNHLDLDAIDWLAELLRAYRGALILISHDRAFIDATATRIVEL
                     DRGVLRSYPGNFSAYEAAKQRELEAEALANARADKLLAQEEIWIRKGVEARRTRSVAR
                     IARLEQLRAQRQARRDQVGQVRLEVATGGQSGKIVAALENVSMRYGERVLIDKLTTTI
                     LRGDKVGLIGPNGSGKTTLLKLILGQLQPTSGTIKLGTNLNVAYFDQMRAGLKLDATL
                     ADTISPGSEWIEIGSTRKHIMSYLGDFLFAPERANAKVMSLSGGERNRLLLARLFALP
                     ANVLVLDEPTNDLDIDTLELLEELLANYAGTVFLVSHDRRFVDNVVTSTLVWEGDVTP
                     GLWREYEGNVEDWQAQRARVRANAPAPVTPAPAPAPAPAAAPVAAPAR"
     gene            complement(1683..2681)
                     /gene="gshB"
                     /locus_tag="VIN75_21480"
     CDS             complement(1683..2681)
                     /gene="gshB"
                     /locus_tag="VIN75_21480"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY8976801.1"
                     /translation="MKLLFVADPLDTFKIYKDSTFAMMREAARRGHTLWATQPVDLSW
                     QRGGRVMARALPITLTGVSGHDEAPWYTTTGVEPVLVAGFDAVLMRKDPPFDSEFFYA
                     THLLSQAEREGAVVLNSPAALREHPEKLAILEFPQFISPTLVTRAAADVRAFHEEHGD
                     IILKPLDGMGGMGIFRVKADGLNLGSIIETLNGNGAQTIMVQKFIPEIVDGDKRVLVI
                     GGQPVPHTLARIPQGGEVRGNLAAGGLGVARPITAREREIAGVIGPKLAARGLLLVGL
                     DVIGDTLTEINVTSPTCFQEITDQSGFDVARMFVDALEAEISGDRSRPTTEGHDPF"
     gene            complement(2819..3982)
                     /locus_tag="VIN75_21485"
     CDS             complement(2819..3982)
                     /locus_tag="VIN75_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY8976802.1"
                     /translation="MLKDVSFSAVVAGFIAVLVGITSSIAIVFQAAQALGATPAQTTS
                     WVWALGVGMAVTSIGLSLWYRQPVLTAWSTPGAALLAGTHGVSLATATGGFIISAVLI
                     TLAGASGLFERLMDRVPVAIAAALLAGVLTRFGLDAALATHGDAWLVGVMALAFVLGR
                     RWWPRYAVPGVLASGIAVAAGTGRISLAAVQWGWARPVLVMPEFSVQAAIGLGLPLFI
                     VTMASQNLPGVAAQRAAGVTAPVSPVIASTGLATLLLAPVGSFGINLAAITAAICMGP
                     EAHPDPKRRYVAPIAAGAFYLVVGLGGGAVVGLMAAFPHALVVAVAGLALLGTIAGAL
                     AQSLAVEKHRDAAALTFLVTLSGVTLAGIGAPFWGVVVGAAALGVQHFRAPAK"
     gene            4181..4867
                     /locus_tag="VIN75_21490"
     CDS             4181..4867
                     /locus_tag="VIN75_21490"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY8976803.1"
                     /translation="MNSTTPNASGAAGLPRAVWIGGGLMALTIIALATTLVVKGNGNS
                     SDTAAADNTVTPLVATTTTPGAPTTEQSSVGKAPANESTKPVHHSTTATHHDTARDNA
                     PAAQQQQVAAAAPVCANCGVVESYAAVQVKGQNNGVGAVAGGLGGALIGSKIAGRGNH
                     TLGGAIGAVGGGLLGNAIESHERTSTAYDVHVRMDDGSVRTIRQATVPTVGQHVNIDG
                     NTMKAVAAQG"
     gene            complement(5014..5775)
                     /locus_tag="VIN75_21495"
     CDS             complement(5014..5775)
                     /locus_tag="VIN75_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY8976804.1"
                     /translation="MDLVVVAAASGLAGFVDAVVGGGGLVLVPALFATYPNAIPATLF
                     GTNKGGAIWGTAWAAVQFARRVTLARVTMAWGMAGALAGSFVGAYTVTLVDAKPLKQA
                     LPFVLLAILLYTLARKELGRHHAPRTQGGAEARIAGLIGLAIGFYDGFFGPGTGSFFV
                     FLFVRILGYDFLHASAAAKMLNTATNLAALVLFTWTGHVWWHVAAVLAVANVTGSLLG
                     TGLALSRGAGFVRAVFIVVVSALILKTGYDGFFHH"
     gene            complement(6149..>7065)
                     /locus_tag="VIN75_21500"
     CDS             complement(6149..>7065)
                     /locus_tag="VIN75_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY8976805.1"
                     /translation="VELRIGSDAPLQVRCRGEVLLSAGAIGSPQILQLSGIGDGARLK
                     AAGVAPRHELPGVGGNLQDHLQVRAVFAVDGTRTLNTLSATVLGKARIAAEYALARSG
                     PMSMAPSQLGAFTRSDPDRRWPNVQYHVQPLSLDAFGEPLHAFEAFTASVCNLNPESR
                     GEVHIRSADPDQAPLIAPRYLATEGDRRVAADSLRLTRRIAAQPALAPFHPREVKPGA
                     QYQTDDELARLAGDIGTTIFHPVGTCRMGRDDDPGAVLDSRLRVRGVAGLRVVDASVM
                     PDITSGNTNSPTLMIAERAAEWTLAPVR"
BASE COUNT         1059 a         2529 c         2518 g          959 t
ORIGIN      
        1 cctggccggt gcggccacgg gcgcggcggc gggggcgggg gccggcgcgg gcgcgggcgt
       61 cacgggcgcg ggggcgttcg cgcggacgcg tgcacgctgc gcctgccagt cctcgacgtt
      121 gccctcgtac tcgcgccaca gccccggcgt cacgtcgcct tcccacacca gggtgctcgt
      181 gacgacgttg tcgacgaagc ggcggtcgtg gctcaccagg aacaccgtgc cggcgtagtt
      241 ggcgagcagt tcctccagga gttcgagcgt gtcgatgtcg aggtcgttgg tcggctcgtc
      301 cagcaccagc acgttggcgg gcagcgcgaa caggcgcgcc agcagcaggc ggttgcgctc
      361 gccgccggac agcgacatca ccttggcgtt cgcgcgctcc ggcgcgaaca ggaagtcgcc
      421 caggtagctc atgatgtgct tgcgcgtcga gccgatctcg atccactcgc tgcccgggct
      481 gatcgtgtcg gccagcgtgg cgtccagctt caggccggcg cgcatctggt cgaagtaggc
      541 gacgttcagg ttggtgccca gcttgatcgt gcccgacgtg ggctgcagct ggcccaggat
      601 cagcttgagc agcgtcgtct tgcccgaacc gttggggccg atcaggccga ccttgtcgcc
      661 acgcaggatg gtcgtcgtga gcttgtcgat cagcacgcgc tcgccgtagc gcatggacac
      721 gttctcgagc gccgcgacga tcttgccgga ctgcccgccc gtggcgacct cgaggcgcac
      781 ctggcccacc tggtcgcgcc gcgcctggcg ctgcgcgcgc agctgctcca gccgcgcgat
      841 gcgggcgacg ctgcgcgtgc ggcgcgcctc gacgcccttg cggatccaga tctcctcctg
      901 cgccagcagc ttgtcggcgc gcgcgttggc cagcgcctcg gcctcgagct cgcgctgctt
      961 ggcggcctcg tacgcgctga agttgccggg atagctgcgc agcacgccgc gatcgagctc
     1021 gacgatgcgc gtggccgtcg cgtcgatgaa ggcgcggtcg tgcgagatca ggatcagcgc
     1081 gccgcggtag gcgcgcagca gctcggcgag ccagtcgatc gcgtccaggt ccaggtggtt
     1141 ggtcggctcg tccagcagca gcacgtcggg cgcggcgacc agcgcctggg ccagcgcgac
     1201 gcgcttgcgc aggccgccgg agagcttgtc gacctgctgc tgcggctcga ggtcgagccg
     1261 gtgcagcgtc tcgtcgacgc ggcgctccca gtcccaaccg ccgagcgcct ccaggcgcgt
     1321 ctgcaggcgg tcgagctcgt cgtggtcgac gccgtcgtgg ttctcgaact tctcgcgcgc
     1381 ctcgcgggcc tcgcgcacgc cctcgctcac ggcgtcgaac acgctgacgc ccggcgcgaa
     1441 cagcggctcc tgcggcacgt aggtgcggcg caggccgcgc tcgacctgca gcaggccgtc
     1501 gtcgggcttg tcgatgcccg ccaggatctt gagcaggctg gacttgccgg tgccgttgcg
     1561 cccgatcagc gcgacgcgtt ccgccgtctc gagcgcgaat tcggcgccat cgagcagcgc
     1621 gacgtgcccg aaggcgaggt gggcgttgga aagagtgagc agggccatcg agaaccttgc
     1681 aatcagaagg ggtcgtggcc ttcggtggtg ggacgggagc ggtcgccgga gatctcggcc
     1741 tcgagcgcgt cgacgaacat cctggcgacg tcgaagcccg actggtccgt gatctcctgg
     1801 aagcaggtcg ggctggtcac gttgatctcg gtgagcgtgt cgccgatgac gtccaggccc
     1861 accagcagca gtccgcgggc cgcgagcttg gggccgatga cgccggcgat ctcgcgctcg
     1921 cgagccgtga tcggccgcgc cacgcccagg cccccggcgg ccaggttgcc gcgcacctcg
     1981 ccgccctgcg ggatgcgggc cagcgtgtgc ggcaccggct ggccgccgat cacgagcacg
     2041 cgcttgtcgc cgtcgacgat ctcggggatg aacttctgca ccatgatcgt ctgcgcgccg
     2101 ttgccgttga gcgtctcgat gatgctgccc aggttcaggc cgtccgcctt gacgcggaag
     2161 atgcccatgc cgcccatgcc gtccaggggc ttgaggatga tgtcgccgtg ctcctcgtgg
     2221 aaggcgcgca cgtcggcggc cgcgcgcgtg accagcgtcg gcgagatgaa ctgcgggaac
     2281 tcgaggatgg ccagcttctc gggatgttcg cgcagcgccg ccggcgagtt cagcaccacc
     2341 gcgccctcgc gctcggcctg gctcagcagg tgggtggcgt agaagaattc gctgtcgaac
     2401 ggcgggtcct tgcgcatcag caccgcgtcg aacccggcca cgagcaccgg ctcgacgccc
     2461 gtcgtcgtgt accagggcgc ctcgtcatgc ccgctcacgc cggtcagcgt gatcggcagc
     2521 gcgcgcgcca tcacgcggcc gccgcgctgc cagctgaggt cgacgggctg cgtggcccac
     2581 agcgtgtggc cgcggcgcgc ggcctcgcgc atcatcgcga aggtcgagtc cttgtagatc
     2641 ttgaacgtat cgagcggatc ggcgacgaac agcagcttca tggggacacc tggtcggggc
     2701 gggtgggcgc cgcgcgcggc ggggcgcagg attcgaggaa gaggacggtg ccacgcgcgc
     2761 atgacgcggg cgcggcacga catgatcgcg cgcgggcgcc cgggccggcg cggcagggct
     2821 atttcgccgg ggcccggaag tgttgcacac cgagcgcggc cgcgccgacg acgacgcccc
     2881 agaacggcgc gccgatgccg gcgagcgtga cgcccgacag ggtcaccagg aacgtcagcg
     2941 ccgcggcgtc gcggtgcttc tcgaccgcca gcgactgggc caatgcgccc gcgatcgtgc
     3001 ccagcagcgc gaggccggcc accgcgacga ccagcgcgtg cgggaacgcc gccatcagcc
     3061 cgacgacggc gccgccgccc agtccgacga cgaggtagaa cgcgccggca gcgatcggcg
     3121 cgacgtagcg ccgcttcggg tccgggtgcg cctccgggcc catgcagatc gccgccgtga
     3181 tcgccgccag gttgatgccg aacgagccga ccggcgccag cagcagcgtc gccaggccgg
     3241 tgctggcgat caccggcgac accggcgccg tcacccccgc cgcccgctgc gccgcgacgc
     3301 ccggcaggtt ctgcgacgcc atcgtcacga tgaacagcgg caaccccagg ccgatcgcgg
     3361 cctggacgct gaactcgggc atcacgagca ccggccgcgc ccagccccac tggacggcgg
     3421 ccagcgagat ccggccggtg cccgcggcca ccgcgatgcc cgaggccagc acgcccggca
     3481 cggcgtagcg cggccaccag cggcggccca gcacgaaggc gagcgccatc acgcccacga
     3541 gccacgcgtc gccgtgcgtg gccagggccg cgtccagccc gaagcgcgtg agcactccgg
     3601 ccagcagagc cgcggcgatc gccaccggca cgcggtccat caggcgctcg aacaggcccg
     3661 aggcgcccgc gagcgtgatg agcaccgccg aaatgatgaa accacccgta gcggtggcca
     3721 gcgagacgcc gtgcgtgccc gccagcaggg cggcgcccgg cgtcgaccac gccgtcagca
     3781 ccggctggcg ataccagagc gacagcccga tgctggtgac cgccatgccc acgcccagcg
     3841 cccagaccca ggacgtggtc tgcgccggcg tggcgccgag cgcctgcgcc gcctggaaca
     3901 cgatcgcgat cgagctcgtg atcccgacca gcaccgcgat gaagccggcg acgacggccg
     3961 agaacgagac atccttgagc atggcgcgaa gggtacccga ggcccggtct cctgctgtcg
     4021 tgtaggacaa ccggccatgg ccgcgtcggc gcgccacggt tcagcagcct tcgctcacgt
     4081 ttcgaagcaa ctcggcactt tggctgacat ccgctcgacg gcgtggcgcg ttcaaatccg
     4141 tgtcagcccg acttacagat gggcttagag gaaaaccgac atgaacagca ccacccccaa
     4201 cgcttccggc gccgccggcc tgccccgcgc cgtctggatc ggcggcggcc tgatggccct
     4261 gaccatcatc gcgctggcga ccaccctggt cgtcaagggc aacggcaaca gcagcgacac
     4321 cgccgccgcc gacaacaccg tcaccccgct ggtggccacc accaccacgc ccggcgctcc
     4381 caccaccgag cagtcgtcgg tcggcaaggc gcccgccaac gaatcgacca agccggtcca
     4441 ccactcgacg accgcgaccc accacgacac cgcccgcgac aacgcgccgg ccgcgcagca
     4501 gcagcaagtc gccgccgccg cgccggtgtg cgccaactgc ggcgtggtcg agagctacgc
     4561 cgccgtgcag gtcaagggcc agaacaacgg tgtcggcgcc gtcgctggcg gcctgggcgg
     4621 tgcgctgatc ggcagcaaga tcgccggccg cggcaaccac acgctgggcg gcgccatcgg
     4681 cgcggtcggc ggcggcctgc tgggcaacgc catcgagtcg cacgagcgca cctccaccgc
     4741 ctacgacgtg cacgtgcgca tggacgacgg cagcgtgcgc accattcgcc aggccaccgt
     4801 gccgaccgtg ggccagcacg tcaacatcga cggcaacacg atgaaggcgg tcgccgcgca
     4861 gggttgatcg cgcgtcgcgc caacgagaac ggcccgcttc gcgggccgtc tttcgttctt
     4921 cttgggtgcc aggtcttgcc aggcaagact tggtgcccgc ttcctggtct tgccaggcaa
     4981 gacctggcac ccgcgtcttt gaacaagcga cgctcaatgg tggaagaacc cgtcgtaccc
     5041 cgtcttcagg atcagcgccg agacgacgac gatgaacacg gcgcgcacga agccggcgcc
     5101 cctggacagc gccaggcccg tgcccagcag gctgccggtc acgttggcca ccgccaggac
     5161 ggcggcgacg tgccaccaga cgtggcccgt ccaggtgaac agcaccagcg cggcgaggtt
     5221 ggtcgccgtg ttgagcatct tggctgcggc cgaggcatgc aggaagtcgt agcccaggat
     5281 gcgcacgaac aggaagacga agaagctgcc ggtgcccgga ccgaagaagc cgtcgtagaa
     5341 cccgatggcc aggccgatga ggccggcgat gcgcgcctcg gcgccgccct gggtgcgcgg
     5401 agcgtggtgc cgtccgagtt ccttcctggc cagcgtgtac aacaggatcg ccagcagcac
     5461 gaagggcagc gcctgcttca gcggcttggc gtcgaccagc gtgaccgtgt acgcgccgac
     5521 gaagctgccc gcgagcgcgc cggccatgcc ccaggccatc gtcacccgcg cgagcgtcac
     5581 gcggcgcgcg aactgcaccg cggcccacgc ggtgccccag atggcgccgc ccttgttggt
     5641 gccgaacagc gtggcgggaa tggcgttcgg gtaggtggcg aagagcgcgg gcaccagcac
     5701 gaggccgccg ccgccgacca ccgcatccac gaagccggcg agcccggagg cggccgccac
     5761 gacgacgaga tcgatcatgg ccccattatc gatgagcagg gccggcccac cggcgcgctg
     5821 cggcatcgcg agtctttcgg acccgaaaaa gacaaaaggg cgcggccggt aggcagcgcc
     5881 cttcgtcttt tgtggtctgg cagtcgccac ggggcgccgc ctgcctggcc cgataagcgt
     5941 tcgagccggc gttatgtctc ctcgtacccc cttcagagtc gcacgcacct cgtgggtgca
     6001 tatgacaaga aagtatttgg actgtaggag cgcgttgttg cggtgcacat ccgggctttc
     6061 ccgctgtcac attgcaccaa caccgttcag gtggcattca tggtgcgttc atggcgtgat
     6121 gaagttggca cgccgtccgg gcgcccgctc accgaaccgg tgccagcgtc cattcggcgg
     6181 cacgctccgc gatcatcagc gtgggcgaat tggtgttgcc gctggtgatg tcgggcatca
     6241 cgctggcgtc caccacccgc agcccggcga cgccgcgcac gcgcaggcgc gagtcgagca
     6301 ccgcgccggg gtcgtcgtcg cgtcccatgc ggcaggtgcc gacggggtgg aagatggtcg
     6361 tgccgatgtc gccggccagg cgcgcgagct cgtcgtccgt ctggtactgg gcgccgggct
     6421 tgacctcgcg cgggtggaat ggcgccagcg ccggttgcgc ggcgatgcgg cgggtcaggc
     6481 gcagcgagtc ggccgcgacg cggcgatcgc cctcggtggc gaggtagcgg ggggcgatca
     6541 gcggcgcctg gtcgggatcg gcgctgcgga tgtggacctc gccgcgcgac tccgggttga
     6601 ggttgcacac gctggccgtg aacgcctcga acgcgtgcag cggctcgccg aaggcgtcca
     6661 gcgacaacgg ctgcacgtgg tactgcacgt tgggccagcg ccggtcggga tcggagcgcg
     6721 tgaacgcgcc cagctgcgat ggcgccatgc tcatcggacc gctgcgcgcc agcgcgtatt
     6781 cggccgcgat gcgcgccttg cccagcaccg tggccgacag cgtgttgagg gtgcgcgtgc
     6841 cgtccaccgc gaacacggcg cgcacctgca ggtgatcctg caggttgccg ccgaccccgg
     6901 gcagctcgtg acgcggcgcc acgccggccg ccttcagccg cgcgccatcg ccgatgcccg
     6961 agagctgcag gatctgcggc gagccgatcg cgccggccga cagcagcacc tcgccgcggc
     7021 agcgcacctg cagcggcgcg tcgctgccga tgcgcagctc gacgc
//
LOCUS       DATCRO010000789         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1044156, whole genome shotgun sequence.
ACCESSION   DATCRO010000789 DATCRO010000000
VERSION     DATCRO010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044156"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..141
                     /locus_tag="VIN75_21505"
     tRNA            54..141
                     /locus_tag="VIN75_21505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ser,seq:cga)
     gene            complement(272..865)
                     /locus_tag="VIN75_21510"
     CDS             complement(272..865)
                     /locus_tag="VIN75_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEY8976806.1"
                     /translation="MSGIGLPIIAGAHRLIVVVGPSGAGKDSVLRGWRALLGEAAPAV
                     ARRVITRAPDTSEAHEAVTPGDFAGLRDRGLLATWWHAHGLDYGVRRSELAPLAGGRW
                     VVLNGSRAHLPALRAQAPGLQVVSVSASEHVLASRLAARGREDAAARDARLARRVADP
                     VDADLSLVNDGDLSACVEALAHWWKTTRRQEPWEHRK"
     gene            complement(862..1557)
                     /locus_tag="VIN75_21515"
     CDS             complement(862..1557)
                     /locus_tag="VIN75_21515"
                     /inference="COORDINATES: protein motif:HMM:NF018053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEY8976807.1"
                     /translation="MSEGPRYALYFAPAAHHPLWTAGCRWLGRDPESGARHAGGLYRA
                     DPCRYGFHATLKPPFALREGTSRAQLLAAVSHFVDEHTPFVMPALHVATLGDFIALRP
                     VDADATARGQPLRGLADDCVRLFDEFRRPASAGETARRAAASLDEAQRENVARWGYAH
                     VFEQWRFHMTLSDGLPRDAQGERVRVELLDAARREFDAVLALPLRCDGVALFVEDSPG
                     ADFRLARRFAFAR"
     gene            complement(1554..2318)
                     /gene="phnF"
                     /locus_tag="VIN75_21520"
     CDS             complement(1554..2318)
                     /gene="phnF"
                     /locus_tag="VIN75_21520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEY8976808.1"
                     /translation="MLKDDDASASSMLDAHDANVARWQAIAAQLRADILAGRVAPGER
                     LPNETALADRFGVHRHTLRQAVQALMREGHLDVRQGSGTFVRELVLDYALQRRTRLTE
                     NLARMGETAGRELLSHAVLPAGEWAEALKLPARSKVALLETRSSVRGRRVGLSTAAFP
                     CPRLADIAAPVARHGSVTRALAELGVADYTRSRSVVSTRLPSQAEADALARPLSQPVL
                     VVQYINVDGAGVPVEAGSTLFAADAVQLVVEPDALA"
     gene            2399..2878
                     /gene="phnG"
                     /locus_tag="VIN75_21525"
     CDS             2399..2878
                     /gene="phnG"
                     /locus_tag="VIN75_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEY8976809.1"
                     /translation="MTDTDRDPAAAAKRRHWLAVLAQAPRALLEQHAAALPTTGFTRL
                     RAPEIGLTMVRARIGNRGDRFNVGDATVTRCVVRHVGADGCVAAGVGYVLGRDEARAE
                     WVASFDALLQQPAHHDALMRDVVAPLAAESARLRAAAAARVAGSRVSFDTLASEIAP"
     gene            2875..3519
                     /gene="phnH"
                     /locus_tag="VIN75_21530"
     CDS             2875..3519
                     /gene="phnH"
                     /locus_tag="VIN75_21530"
                     /inference="COORDINATES: protein motif:HMM:NF017645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEY8976810.1"
                     /translation="MSAVLARMGAGFADPVHGAQQAFRALLTAMSEPGRVLALPAAAI
                     AGLESSGNEARPPFDVALAATLLTLLDADTPAHLAGALDTGDARAWLRFHTGARTLPQ
                     ATAMTAALARDVDAALWEALDPGTDDAPQASATLFVQVGALSEQPLPGTVALKLRGAG
                     IRTSRDLAVAGLSPAFWRWRAALRAELPRGVDLVLACGARLAAIPRSTHVESEA"
     gene            3521..>3685
                     /locus_tag="VIN75_21535"
     CDS             3521..>3685
                     /locus_tag="VIN75_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEY8976811.1"
                     /translation="MYIAVKGGERAIEASRRLLAAARRGDADVPELEVAQIEQQMGLL
                     VGRVMAEASLY"
BASE COUNT          497 a         1376 c         1313 g          499 t
ORIGIN      
        1 aattccgtgc tagagtcacg gactccgctc aattgaccag cggcaccgaa tcaggagagg
       61 tggcagagtg gtcgattgta cctgactcga aatcaggcgt accgcaaggt accgtgggtt
      121 cgaatcccac cctctccgcc aagaagtacg aaaacacggg ccctcgcggc ccgtttttct
      181 tttcgttcca ccaatccttc taccgatcgg cggggtgatc gagatggcat tggttccggg
      241 gggcgacgcc tgtcgagcgc aaccaaactg gtcatttgcg gtgttcccag ggctcttggc
      301 ggcgcgttgt tttccaccag tgcgccagtg cctccacgca tgcgctcaga tcgccgtcgt
      361 tgaccaggct caggtcggca tccactggat ctgcgaccct ccgcgccagg cgcgcgtcgc
      421 gcgcggccgc gtcctcgcgc ccccgcgcgg ccaggcgtga cgcgagcacg tgttccgacg
      481 cgctcaccga tacgacctgc aggcccggtg cctgcgcgcg caacgcgggc aggtgcgctc
      541 gcgagccgtt gagcacgacc cagcggccac cggccagggg cgcgagttcg ctgcggcgca
      601 cgccgtagtc caggccgtgc gcatgccacc aggtcgcgag caggccgcgg tcgcgcaggc
      661 cggcgaagtc gccgggcgtg acggcctcgt gcgcctccga agtatccggt gcgcgggtga
      721 tcacgcgccg cgcgaccgcg ggggccgctt cgcccagcaa tgcgcgccag ccgcgcagca
      781 cgctgtcctt gcccgcgccc gacggcccga ccacgacgat caggcgatgc gctcccgcga
      841 tgatcggcag gccgatcccg ctcatcgtgc gaacgcgaaa cgtcgcgcga ggcggaagtc
      901 cgcaccgggg gagtcctcga cgaacagcgc gacgccgtcg cagcgcagcg gcagggcgag
      961 cacggcgtcg aattcgcggc gcgccgcgtc gagcagctcg acgcgcaccc gctcgccctg
     1021 cgcatcgcgc ggcaggccat cgctgagcgt catgtggaag cgccattgct cgaacacatg
     1081 ggcgtagccc cagcgcgcga cgttctcgcg ctgcgcctcg tcgaggctcg cggccgcccg
     1141 gcgcgcggtc tcgccggccg aggcgggccg tcggaactcg tcgaacaggc gaacgcagtc
     1201 gtctgcgaga cctcgcagcg gctggccgcg tgcggtcgca tcggcatcga ccgggcgcag
     1261 cgcgatgaaa tcgcccagcg tcgcgacgtg cagggccggc atcacgaacg gcgtgtgctc
     1321 gtcgacgaag tgcgacaccg cggccagcaa ctgcgcccgc gacgttccct cgcgcagcgc
     1381 gaacggcggc ttgagcgtgg cgtggaatcc atagcggcac gggtcggcgc gatacaggcc
     1441 gcccgcatgc cgcgcgccgg actccgggtc gcggccgagc cagcggcagc cggccgtcca
     1501 caacgggtgg tgcgcggcgg gcgcgaaata caacgcgtag cgcgggcctt cgctcatgcg
     1561 agcgcgtccg gttcgaccac cagctgcacc gcgtcggccg cgaacagcgt gctgcccgcc
     1621 tccaccggca cgcccgcgcc gtcgacattg atgtactgca cgaccagcac gggctgcgac
     1681 aacggccgcg cgagcgcgtc ggcctcggcc tgcgacggca ggcgggtcga caccacgctg
     1741 cgcgagcgcg tgtagtcggc cacgccgagc tcggcgagcg cgcgcgtgac gctgccgtgg
     1801 cgcgccacgg gcgcggcgat gtccgcgagg cgcggacatg ggaatgccgc tgtggacagc
     1861 cccacgcgac ggccgcgcac cgacgagcgc gtctccagca gtgccacctt cgaacgcgcc
     1921 ggcagcttca gtgcctcggc ccattcgccg gccggcagca ccgcatgcga cagcagctcg
     1981 cgcccggccg tctcgcccat gcgcgcgagg ttctcggtca ggcgcgttcg gcgctgcaag
     2041 gcgtagtcca gcaccagctc gcgcacgaag gtgccgctgc cctggcgcac gtccaggtgg
     2101 ccctcgcgca tcagcgcctg caccgcctgg cgcagcgtgt ggcggtggac gccgaagcgg
     2161 tcggccagcg ccgtctcgtt gggcaggcgc tcgccgggcg cgacgcggcc ggcgaggatg
     2221 tcagcgcgca gctgcgcggc gatcgcctgc cagcgcgcga cgttggcgtc gtgcgcatcg
     2281 agcatcgagg aagctgaagc gtcgtcgtcc ttgagcatgt cgtcaccgga gggtcaagaa
     2341 actgtcatcg ggtctgtcga tactggctcg catcttcagt catctagatg acgtcaccat
     2401 gaccgacacc gatcgagacc ccgccgcggc ggccaagcgc cggcactggc tggccgtgct
     2461 ggcgcaggcg ccgcgcgcgc tgctcgagca gcacgccgcc gcgctgccca cgaccggctt
     2521 cacgcggctg cgcgctccgg agatcggcct gacgatggta cgcgcccgca tcggcaaccg
     2581 gggcgaccgt ttcaacgtcg gcgacgccac cgtcacgcgc tgcgtcgtgc gccacgtcgg
     2641 cgcggatggc tgcgtcgcgg ccggcgtcgg ctacgtgctg ggccgcgacg aggcgcgcgc
     2701 cgaatgggtg gcgtccttcg acgccttgct gcagcagccc gcgcaccacg acgcgctgat
     2761 gcgcgacgtc gtcgcgccgc tcgctgcgga gtccgcgcgc ctgcgtgccg ccgccgcggc
     2821 gcgcgtcgcc ggttcgcgcg tctcgttcga cacgctcgcc tcggagatcg cgccatgagc
     2881 gctgtccttg cccgcatggg cgccggcttt gccgatccgg tgcacggcgc tcagcaggcc
     2941 ttccgcgccc tgctgacggc gatgtccgag cccggacgcg ttctggcgct gcccgccgcc
     3001 gcgatcgccg gcctcgagtc gtccgggaac gaggcgcgcc cgccgttcga cgtcgctctg
     3061 gccgcgacgc tgctgacgct gctcgacgca gacacccccg cccacctcgc cggtgccctg
     3121 gacaccggcg acgcgcgcgc ctggctgcgc ttccacaccg gcgcccgcac gctgccgcag
     3181 gccacggcga tgacggcggc actggcgcgc gacgtcgacg cggcgctgtg ggaggcgctc
     3241 gatccgggca ccgacgatgc gccgcaggcc agcgccacgc tgttcgtgca ggtgggtgcg
     3301 ctgtccgagc agccgctgcc cggcaccgtc gcactcaagc tgcgcggcgc cggcatccgg
     3361 acgtcgcgcg acctcgccgt cgcgggcctg tcgcccgcgt tctggcgctg gcgcgcggcg
     3421 ctgcgcgccg agttgccgcg cggcgtcgac ctcgtcctcg cctgcggcgc gcgcctggcc
     3481 gccattcccc gcagcaccca cgtcgaaagc gaggcctgag atgtacatcg cggtcaaggg
     3541 cggcgaacgc gccatcgagg cctcgcggcg gctgctcgcg gccgcgcggc gcggcgacgc
     3601 ggacgtgccc gaactcgagg tcgcgcagat cgagcagcag atgggcctgc tggtgggccg
     3661 cgtcatggcg gaggcctcgc tgtac
//
LOCUS       DATCRO010000790         4738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_976434, whole genome shotgun sequence.
ACCESSION   DATCRO010000790 DATCRO010000000
VERSION     DATCRO010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976434"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VIN75_21540"
     CDS             <1..510
                     /locus_tag="VIN75_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8976812.1"
                     /translation="AAAAAGYSHALQIDADGQHDAGDIPRFLAAAAADPAAVICGVPV
                     YDESVPTGRLVGRYATHIWVWINSLSLQIRDSMCGFRVYPLAPVVKLLDEARLGRRMD
                     FDPEVLVRLHWRGLRIVNIPTRVTYPQDGLSHFRLGLDNWLISRMHARLAVGMLLRSP
                     RLLWRKVVR"
     gene            507..1502
                     /locus_tag="VIN75_21545"
     CDS             507..1502
                     /locus_tag="VIN75_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976813.1"
                     /translation="MSATSQHAAGAVQRHWSAIGEETFAGGMWLMYALFRVGGRLPFR
                     LVLYPVVLWYWAANRAAREASLQYLRRLQAATGALGHEPGRRDTLRHFFSFAETILDK
                     MLAVGGRYKLDTIRFVGREPIEQMVQARQGGLFVTAHVGCLEMCQASADATPGLKLTV
                     LVHTVHAERFNRMLERLSPGRGISLMQVTEISPATAVLLSERVARGEFVAIAGDRVPV
                     MAGRATAVEVPFLGHDAPLPTGAYMLAALLKCPLFSMGCIRQGDTHEVVFERLAERVV
                     LPRGDRLSACAGYAAEFARGIERLLARAPYEWFNFFSFWNQPGALAARRKSERHD"
     gene            1495..3045
                     /locus_tag="VIN75_21550"
     CDS             1495..3045
                     /locus_tag="VIN75_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEY8976814.1"
                     /translation="MTESRSIRFDGSALTIEDVCLLAEMKADASLSEDPAFRARIRRG
                     SEFLERLLREDGVVYGVTTGYGDSCTVVIPPELVPELPHHLFAYHGVGLGRMLDPAET
                     RAVLAARLQSLAQGVSGVSVELLDQLAAFLEHDVLPQIPAEGSVGASGDLTPLSYVAA
                     ALCGERNVLFQGRIQPAAAALETLRRQPLRLRPKEGLAIMNGTAVMTALACLAWQRAD
                     YVCKLASRLTACNVLASAGNAHHFDEALFAVKPHAGQQRVAARVRADLAAESDKPAAR
                     NEQRLQDRYSLRCAPHVIGVLEDALPFLRQLIESELNSANDNPIIDGDNERVLHGGHF
                     YGGHVAFAMDSLKNAVANVADLLDRQLALLVDTRYNHGLPSNLSGATGVRAPINHGLK
                     ALQISVSAWTAEALKQTMPASVFSRSTECHNQDKVSMGTIAARDCMRVLELTEQVCAA
                     MLVAGRQGVALRCAQDDVVPGQVLQAFQADLERRIPFVAEDRALDADLIALLAALRRR
                     EWALYGDE"
     gene            3038..3475
                     /locus_tag="VIN75_21555"
     CDS             3038..3475
                     /locus_tag="VIN75_21555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8976815.1"
                     /translation="MSESRKPATPDLSHEIEVVPPFFDIDSMDIVWHGHYVKYLELAR
                     CALLEKFDYGYTRMRESGYAWPIVDLRLKYVRPATFNQPLVVHAEIVEWENRLKFEYV
                     IRDKATGEKVNAAMSIQVAVDMATRQMQYVCPRVLWERLGVAS"
     gene            3472..4131
                     /locus_tag="VIN75_21560"
     CDS             3472..4131
                     /locus_tag="VIN75_21560"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY8976816.1"
                     /translation="MSGCVRVIALVVLALVAAASFAATPAASASASASPAGDSLKLVH
                     DRLKTTPVLKGEFEQTKTLKGFRNALVSTGEFLVARGQGVWWHTRQPFESTLVVTRTK
                     LFTRNPDGSTSNLMDAQAEPGVRQVNELVFSLLAADIDVLADKFTVAVQPVGAAGWTA
                     TLTPRDPAIAKFLARATLAGERNVQSVRIEEARGDTTQIRFSRQAPATVLTPDEAARF
                     Q"
     gene            4230..>4738
                     /locus_tag="VIN75_21565"
     CDS             4230..>4738
                     /locus_tag="VIN75_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976817.1"
                     /translation="MRPLPWQVAAWLWLACCVVVAAHQFAFWRHAHFDTDVMALLPQD
                     EQAPEVGIATRQLADQATRQVVVMIGAPDWASAQKAAAAWEQGAMAEGGPLKGSAIAG
                     VDSLQDTLAFYAPYRDRLLTPAQRAQLEHAAPAALVQSALAALAQPGGGYRLADWNAD
                     PLALWPQWWL"
BASE COUNT          629 a         1717 c         1691 g          701 t
ORIGIN      
        1 gccgccgcgg ccgcggggta ttcgcacgcg ctgcagatcg acgccgacgg ccagcacgac
       61 gccggcgaca tcccgcggtt cctggccgcc gcggccgccg atcccgccgc ggtgatctgc
      121 ggcgtgccgg tctacgacga gtcggtgccg acggggcgcc tggtcgggcg ctacgccacg
      181 cacatctggg tgtggatcaa ttcgctgtcg ctgcagatcc gcgactcgat gtgcggcttc
      241 cgcgtctatc cgctggcgcc ggtcgtcaag ctgctggacg aggcgcgcct gggccggcgc
      301 atggacttcg accccgaggt gctggtgcgc ctgcactggc gcgggctgcg catcgtgaac
      361 atccccacgc gcgtcacgta cccgcaggac gggctgtcgc acttccgcct gggcctggac
      421 aactggctca tctccaggat gcacgcgcgg ctcgccgtcg gcatgctgct gcgctcgccg
      481 cggctgctgt ggcgcaaggt ggtgcgatga gcgcgacgtc gcaacatgcc gcgggcgccg
      541 tgcagcgcca ctggtcggcc atcggcgagg agaccttcgc cggcggcatg tggctgatgt
      601 acgcgctgtt ccgcgtcggc gggcggctgc cgttccggct cgtgctgtac ccggtcgtgc
      661 tctggtactg ggccgccaac cgcgccgcgc gcgaagcctc gctgcaatac ctgcgccgat
      721 tgcaggcggc caccggcgcg ctgggccacg agcccggccg gcgcgacacg ctgcgccact
      781 tcttctcgtt cgccgagacc atcctggaca agatgctggc cgtgggcggc cgctacaagc
      841 tcgacacgat ccgcttcgtc ggccgcgaac cgatcgagca gatggtgcag gcccggcagg
      901 gcgggctgtt cgtgacggcg cacgtgggct gcctggagat gtgccaggcc tcggccgacg
      961 ccacgcccgg cctgaaactg acggtgctcg tgcatacggt gcacgccgag cgcttcaacc
     1021 gcatgctcga acgcctgtcg ccggggcgcg gcatctcgct gatgcaggtc accgagatct
     1081 cgccggccac cgcggtgctg ctgtccgagc gcgtggcgcg cggcgagttc gtcgccatcg
     1141 ccggcgatcg cgtgcccgtg atggcgggcc gcgcgacggc cgtcgaggtg ccgttcctcg
     1201 gccacgacgc gccgctgccc accggcgcct acatgctcgc cgcgctgctg aagtgcccgc
     1261 tgttctcgat gggctgcatc cgccagggcg acacgcacga ggtcgtgttc gagcggctgg
     1321 ccgagcgcgt cgtgctgccg cgcggcgatc gcctgtcggc ctgcgccggc tacgccgcgg
     1381 agttcgcccg cggcatcgag cgcctgctgg cgcgcgcgcc ctacgaatgg ttcaatttct
     1441 tttccttctg gaatcaacct ggagcgctcg ccgctcgccg aaagtctgag cgccatgact
     1501 gaatcccgct ccatccgctt cgacggcagt gcgctgacca tcgaggacgt gtgcctgctc
     1561 gccgagatga aggccgacgc ctcgctgtcc gaggatccgg cgttccgcgc gcgcatccgc
     1621 cgcggctcgg aattcctcga gcggctgctg cgcgaggacg gcgtcgtcta cggcgtcacc
     1681 accggctacg gcgattcgtg caccgtcgtc atcccgccgg agctggtgcc cgagctgccg
     1741 caccacctgt tcgcgtacca cggcgtcggc ctggggcgca tgctcgatcc ggccgagacg
     1801 cgcgccgtgc tggcggcgcg cctgcagtcg ctggcgcagg gcgtctcggg cgtcagcgtc
     1861 gagctgctcg accagctggc tgcgttcctc gagcacgacg tcctgccgca gattcccgcc
     1921 gagggctccg tcggcgccag cggcgacctg acgccgctgt cgtacgtggc ggccgcgctg
     1981 tgcggcgagc gcaacgtgct gttccagggc cgcatccagc cggccgccgc ggcgctggag
     2041 acgctgcgcc gccagccgct gcggctgcgg cccaaggaag gcctggccat catgaatggc
     2101 acggccgtga tgaccgcgct cgcgtgcctg gcctggcagc gcgccgacta cgtctgcaag
     2161 ctcgcgtcgc gcctgacggc gtgcaacgtg ctggccagcg ccggcaacgc gcaccacttc
     2221 gacgaggcgc tgttcgccgt caagccgcat gccggccagc agcgcgtggc cgcccgcgtg
     2281 cgcgccgacc tcgcggccga gtccgacaag cccgccgcgc gcaacgagca gcgcctgcag
     2341 gaccgctact cgctgcgctg cgcgccgcac gtcatcggcg tgctggagga cgcgctgccg
     2401 ttcctgcgcc agctcatcga aagcgagctc aactcggcca acgacaaccc gatcatcgac
     2461 ggcgacaacg agcgcgtgct gcacggcggc cacttctacg gcggccatgt cgcgttcgcc
     2521 atggacagcc tgaagaacgc ggtggccaac gtcgccgacc tgctcgatcg ccagctcgcg
     2581 ctgctggtcg acacccgcta caaccacggc ctgccgtcca acctgtcggg cgccaccggc
     2641 gtgcgcgcgc cgatcaacca cggcctgaag gcgctgcaga tcagcgtctc cgcctggacg
     2701 gccgaggccc tgaagcagac gatgccggcg agcgtgttct cgcgctccac cgagtgccac
     2761 aaccaggaca aggtgagcat gggcacgatc gccgcgcgcg actgcatgcg cgtgctggag
     2821 ctgaccgagc aggtgtgcgc cgccatgctg gtggccgggc gccagggcgt cgcgctgcgc
     2881 tgcgcgcagg acgacgtggt gcctggccag gtgctgcagg cgttccaggc cgacctcgag
     2941 cgccggatcc cgttcgtggc cgaggatcgc gcgctcgacg ccgacctgat cgcgctgctg
     3001 gccgcgctgc gccggcgcga gtgggcgcta tacggcgatg agtgagtccc gcaagccggc
     3061 cacgccggac ctgtcgcacg agatcgaggt cgtgccgccg ttcttcgaca tcgactcgat
     3121 ggacatcgtc tggcacggcc actacgtcaa gtacctcgag ctcgcgcgct gcgcgctgct
     3181 ggagaagttc gactacggct acacgcggat gcgcgagtcc ggctacgcgt ggcccatcgt
     3241 cgacctgcgc ctgaagtacg tgcgtccggc cacgttcaac cagccgctgg tcgtgcacgc
     3301 cgagatcgtc gagtgggaga accgcctgaa gttcgagtac gtgatccgcg acaaggcgac
     3361 gggcgagaag gtcaacgccg cgatgtcgat ccaggtggcg gtggacatgg ccacgcggca
     3421 gatgcagtac gtctgcccgc gcgtgctgtg ggaacggctg ggggtggcgt cgtgagcggc
     3481 tgcgtccggg tgattgcgct cgtggtgctc gcgctggtcg ctgccgcgtc gttcgccgcg
     3541 acgccggcgg cttcggcctc cgcgtccgcg tcgcccgcgg gcgactcgct caagctggtc
     3601 cacgaccggc tgaagaccac gccggtgctc aagggcgagt tcgagcagac caagacgctc
     3661 aagggcttcc gcaacgcgct cgtctccacg ggcgagttcc tcgtcgcgcg cggccagggc
     3721 gtgtggtggc acacccggca gccgttcgag tcgacgctgg tcgtgacgcg cacgaagctg
     3781 ttcacgcgca acccggacgg cagcacgagc aacctgatgg acgcgcaggc cgagccgggc
     3841 gtgcgccagg tcaacgagct ggtcttctcg ctgctggccg ccgacatcga cgtgctggcc
     3901 gacaagttca ccgtcgccgt gcagcccgtg ggtgccgccg gctggaccgc gacgctgacg
     3961 ccgcgcgacc cggccatcgc caagttcctc gcgcgcgcca cgctcgctgg cgagcgcaac
     4021 gtgcagtccg tgcgcatcga ggaggcgcgc ggcgacacga cgcagatccg cttctcgcgc
     4081 caggcgcccg ccacggtgct gacgcccgac gaggcggcgc gcttccaatg agtccgcccg
     4141 gccgcccgaa ggacgaattg cccccgcctg gcgggggggc gcacagcgcc aggggtgcgg
     4201 ctgcgcactt cgcggacgcg acgcggcccg tgcgccccct gccgtggcag gtggcggcgt
     4261 ggctgtggct cgcgtgctgc gtcgtcgtgg cggcgcacca gttcgcgttc tggcgccacg
     4321 cgcacttcga caccgacgtg atggcgctgc tgccgcagga cgagcaggcg cccgaggtgg
     4381 gcatcgccac gcgccagctc gcggaccagg cgacgcgcca ggtcgtcgtg atgatcggcg
     4441 cgcccgattg ggcgagcgcg cagaaggccg ccgcggcgtg ggagcagggc gccatggccg
     4501 agggtggtcc gctgaagggc agcgcgatcg ccggcgtcga ttcgctgcag gacacgctcg
     4561 ccttctacgc gccgtaccgc gaccgcctgc tgacgcccgc gcagcgcgcg cagctcgaac
     4621 atgccgcgcc cgcagcgctg gtgcagtcgg cgctcgccgc gctggcccaa cccggcggcg
     4681 gctatcggct cgccgactgg aatgcggacc cgctcgcgct gtggccgcag tggtggct
//
LOCUS       DATCRO010000791         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_864327, whole genome shotgun sequence.
ACCESSION   DATCRO010000791 DATCRO010000000
VERSION     DATCRO010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864327"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /locus_tag="VIN75_21570"
     CDS             <1..2242
                     /locus_tag="VIN75_21570"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEY8976818.1"
                     /translation="TVAIFQIQGSGATSPYVGQAVTTGGVVTKVNNNGFFMQDLVGDG
                     NPATSDGIFVFTGSAPTVGAGQLVQVGGTVQEFNVGAATNADTAAHKVTELGNVTSIM
                     VTGSGYTIAPVQLQFPLASQDAMEAYEGMLVTISGPLTVQQNYFLGRFGELTLGAGGR
                     LWTPTNVYRPGAQAQALNSDNIRRTILLDDGSSLQNPNPTPYLAPDNTVRAGDTVASV
                     TGVVDYGLTTSANTDPGAYKLHPTQPVTFTRANPRTTMADDVGGNLRIGSANVENFFT
                     TLDDGTSKCPPSNTADDCRGANNAAEFTRQRTKVVEELVGMNADAVALMELQNNGATA
                     IQNLVDALNARLGATVYARVPFAATGGGTDAIQVGMIYKPSRLSLAGAALSDTAAINN
                     RAPMAQTFAAPNGEKFTLIANHLKSKGCSDFTAGPGDADSGDGQGCFNARRVQQADEL
                     RSFVAQVQSISGVADTLLVGDFNAYAKEDPIADLTGNGFVDQIGRFLDVPGANSYGYS
                     YVFNGSSGRLDHALASNTLSPKVTGVTEWHINADEPLVIDYNQEFKQPACATCGPDYY
                     TPTPYRASDHDPIVLGLNLVHAINGTAGADVIVGTPGDDLITGGAGADRLTGNGGRDV
                     FVYTSMRDAADTITDFTPGDDRLDLTALLASIGASPATAWGNGVVTLAASSNNTVVLI
                     DADGKAGPAAGRPLVTLLNVSPAAIDPLRDLGLGTPAAVSAAVKASAKAATLRTMTAA
                     RSTTKK"
     gene            2250..2996
                     /locus_tag="VIN75_21575"
     CDS             2250..2996
                     /locus_tag="VIN75_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8976819.1"
                     /translation="MKQMIPAALSALALAIAAGPAAAQVVNGDFNGLGGWSAGGDAAA
                     TGGHLVLTTASASQQDDADAGRPAGARNVSGNEPLAAGGGPGSLEDFVGVPAGAIDPD
                     PAGFVAAYEGSAASQSFFAAAGSRLSFMWDLDTLDTRHDAAFADVAFVVIDGQLITLG
                     NTLDATSAIAGGDLASHTGWAGFSTTFANAGTHTISFGVADVGDFIDTSTLSVSGVGV
                     AIPAVPESPSLALMAAGLGLLGLQARRRKN"
     gene            3130..>3417
                     /locus_tag="VIN75_21580"
     CDS             3130..>3417
                     /locus_tag="VIN75_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HEY8976820.1"
                     /translation="MNIHKNARLTYLRRLEMVHDMTDRGLGASEAAAKHGVTAVTARK
                     WHARYLAGGAAALLDKSSRPTRSPRAIDAKVASTIVELRRKLHLQSNIAAYM"
BASE COUNT          582 a         1268 c         1120 g          447 t
ORIGIN      
        1 gaccgtcgcg atcttccaga tccagggatc gggcgcgacg agtccgtacg tcggccaggc
       61 cgtcaccacg ggcggcgtcg tgaccaaggt caacaacaac ggtttcttca tgcaggatct
      121 cgtgggcgac ggcaaccccg cgacgtcgga cggcatcttc gtgttcaccg gcagcgcgcc
      181 cacggtcggc gccggccagc tggtgcaggt cggcggtacg gtgcaggagt tcaacgtcgg
      241 cgccgcgacc aacgcggaca ccgccgccca caaggtgacc gagctgggca acgtcaccag
      301 catcatggtg acgggcagcg gctacacgat cgcgccggtg caactgcagt tcccgctggc
      361 gagccaggac gccatggagg cctacgaagg catgctggtg acgatcagcg gcccgctgac
      421 cgtgcagcag aactacttcc tcggccgctt cggcgagctg acgctgggcg ccggcggccg
      481 cctgtggacg cccaccaacg tctaccgccc cggcgcgcag gcgcaggcgc tgaactcgga
      541 caacatccgg cgcaccatcc tgctggacga cggcagcagc ctgcagaacc cgaacccgac
      601 gccctacctc gcgccggaca acaccgtgcg cgccggcgac accgtggcct cggtcaccgg
      661 cgtcgtcgac tacggcctga ccacctcggc caacaccgat ccgggggcct acaagctcca
      721 cccgacgcag ccggtgacgt tcacgcgggc caacccgcgc acgacgatgg ccgacgacgt
      781 cggcggcaac ctgcgcatcg gctcggccaa cgtcgagaac ttcttcacca cgctcgacga
      841 cggcaccagc aagtgcccgc cgtccaacac ggccgacgac tgccgcggcg ccaacaacgc
      901 cgcagagttc acgcgccagc gcaccaaggt cgtcgaggaa ctcgtcggca tgaacgccga
      961 cgccgtggcg ctgatggagc tgcagaacaa cggcgccacc gcgatccaga acctggtcga
     1021 cgcgctcaac gcgcgcctgg gcgccaccgt ctacgcgcgc gtgccgttcg cggccacggg
     1081 cggcggcacc gacgccatcc aggtcggcat gatctacaag ccgtcgcgcc tgtcgctggc
     1141 gggcgccgcg ctgagcgaca ccgccgccat caacaaccgc gcgccgatgg cgcagacgtt
     1201 cgcggcgccg aacggcgaga agttcacgtt gatcgccaac cacctgaagt ccaagggctg
     1261 cagcgacttc accgccggtc ccggcgatgc cgactccggc gacggccagg gctgcttcaa
     1321 cgcgcgccgc gtgcagcagg ccgacgagct gcgcagcttc gtcgcgcagg tgcagtccat
     1381 cagcggcgtg gccgacacgc tgctcgtggg cgacttcaac gcctacgcca aggaagaccc
     1441 gatcgccgac ctgaccggca acggcttcgt cgaccagatc gggcgcttcc tggacgttcc
     1501 cggcgcgaac tcgtacggct actcctacgt cttcaacggc agctccggcc gcctcgacca
     1561 cgcgctcgcg agcaacacgc tgtcgcccaa ggtgaccggc gtgaccgagt ggcacatcaa
     1621 cgccgacgag ccgctggtga tcgactacaa ccaggagttc aagcagcccg cctgcgccac
     1681 ctgcggcccg gactactaca ccccgacgcc gtaccgcgcc tcggaccacg acccgatcgt
     1741 cctcggcctg aacctcgtgc acgccatcaa cggcacggcc ggcgccgacg tcatcgtggg
     1801 caccccgggc gacgacctca tcaccggcgg cgcgggcgcc gaccgcctga cgggcaacgg
     1861 cggacgcgac gtgttcgtct acacgtcgat gcgcgacgcg gccgacacca tcaccgactt
     1921 cacgcccggc gacgaccggc tcgacctcac cgcgctgctg gccagcatcg gcgcgagccc
     1981 ggccacggcc tggggcaacg gcgtcgtgac gctggcggcg tcgagcaaca acacggtggt
     2041 gctgatcgac gccgacggca aggccggccc ggccgccggc cgtccgctgg tcacgctgct
     2101 gaacgtgagc cccgccgcga tcgacccgct gcgcgacctc ggcctgggca cgcctgcggc
     2161 cgtgtccgcc gccgtcaagg cctcggccaa ggccgccacg ctgcgcacga tgaccgccgc
     2221 gcgctccacc acgaagaagt gagaccgaca tgaagcaaat gattcccgcc gcgttgtcgg
     2281 ccctcgccct cgcgatcgcc gccggccccg cggccgcgca ggtggtcaac ggcgacttca
     2341 acggcctcgg cggctggtcg gcgggcggcg acgccgcggc caccggtggc cacctggtgc
     2401 tgacgaccgc gtcggcctcg caacaggacg acgccgacgc cggccggccc gccggcgcgc
     2461 gcaacgtctc gggcaacgaa ccgctcgcgg ccggcggcgg cccgggcagc ctcgaggact
     2521 tcgtcggcgt ccccgccggc gcgatcgatc ccgatccggc cggcttcgtg gccgcctacg
     2581 agggctccgc ggcgtcgcag agcttcttcg cggcagccgg cagccggctg tcgttcatgt
     2641 gggacctgga caccctcgac acgcgccacg acgccgcctt cgcggacgtg gcgttcgtcg
     2701 tcatcgatgg acagctcatc acgctgggca acacgctgga cgcgacgtcg gcaatcgccg
     2761 gcggcgatct tgcgtcgcac acgggctggg cgggcttcag cacgaccttc gccaacgcag
     2821 gcacgcacac gatctcgttc ggcgtggccg acgtcggcga cttcatcgac acgtcgacgc
     2881 tgtcggtcag cggcgtgggc gtggcgatcc cggcggtgcc cgaatcgccg tcgctggcgc
     2941 tgatggcggc ggggctggga ctgctgggcc tgcaggcgcg gcgtcgcaag aactgaagca
     3001 ggccgcgctc gcgagacgca gggcgggtta gctgtgaagg ttcaataggt tcttgcgggt
     3061 gttcacccgg caaggttttg agagactgga aatcgccaaa cccccagtca actccgaacc
     3121 ggaggccgga tgaacatcca taagaatgcc cgattgacgt acctgcgccg tctggagatg
     3181 gtgcatgaca tgacagatcg tggcttgggc gcgtcagaag ccgcggccaa gcacggcgtg
     3241 accgccgtca cggctcgcaa atggcacgcg cgctaccttg ccggcggagc cgcggcctta
     3301 ctggacaagt cctcccggcc aacaagatcg ccaagggcca tcgacgccaa agtcgcttcg
     3361 accattgtcg agcttcgacg aaagctgcat ctgcaatcga acatcgctgc ctacatg
//
LOCUS       DATCRO010000792         9250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_87738, whole genome shotgun sequence.
ACCESSION   DATCRO010000792 DATCRO010000000
VERSION     DATCRO010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87738"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VIN75_21585"
     CDS             complement(<1..575)
                     /locus_tag="VIN75_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8976821.1"
                     /translation="MQTLQWQRRGLSDRGMAWLFVTPTIVLLLAFNIFPLFWTILLSL
                     TNYRANHPGADLVWTGIENYRRVLDNEEIWAHLQATARFLVCSITLQLLLGFGLALML
                     NRRFRTHGFWSTAILLPMMLAPAVVGTFWKYFFQPQYGIFNQALRLFGAPEDLSMLAD
                     SSLAPWAIVLVDTWMWTPYVMLLLLAGLRSIP"
     gene            complement(710..2044)
                     /locus_tag="VIN75_21590"
     CDS             complement(710..2044)
                     /locus_tag="VIN75_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8976822.1"
                     /translation="MTTTKKAAAAAIAAFLGTAMCTGAQAADQKQLTLCWAAWDPANA
                     LVELSKDFTAKTGIQMKYEFVPWPNYAQRMLNELNSKGKLCDLLIGDSQWIGGAATEG
                     HYVKLNDFFDKNGIKMADFMPATVLGYSEWPKGTPNYWALPAMGDAVAFSYRKDWFAR
                     PELQAEFKKKYGHDLAPPATWPEFKQIGEFFQGRVIDGKKVYGAALYTERGSEGITMG
                     VTNTMYAYGFEYSNPKKPYDMKGWVNSAGAVKGLQFYKELYDCCTPPGHSNAYMTENL
                     DAYKSGQVAMQMQFIAFFPGIAKDPQVGGDKTGFFPIPRGDKQFAMLGGQGISVVKYS
                     DKKDMALEYMKWFAQPAVQKKWWDAGGFTCLSSVLNAPGFDKSTPYAKVFLDSMKMVK
                     DFWAEPSYAALLEAMQSRVHDYVVAGKGTPQAALDSLEKDWEETFREDGKLK"
     gene            2300..3328
                     /locus_tag="VIN75_21595"
     CDS             2300..3328
                     /locus_tag="VIN75_21595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8976823.1"
                     /translation="MANPLTIAQIAEAAGVSTATVDRVLNERAGVNPATVGKVREAIA
                     ALGGGTPARGRPRSSSNYRFGFVLPSARRGFFDQVDRVIAQTAGEFRHQHITEVTHRL
                     PAADVNQFAAELTKLSDLDGLALIAPDLPPVKLAINELVRAGVHVVTLFSDVPGSMRA
                     TAIGADNRAAGRTAGLLIGNSLPRDAGRTCALLSPATRYAAEIDRRIGFQQVMEERFP
                     DAVVTRLFEMPEADDAAYEMARELLTPVAADLAGVYNVGPASFGVARALAEMKTTRPP
                     MFVANDLLEVHRTMLMSGALSYVINQDLYSSISNASRVLRGLCDGVRGALSLGNPRVE
                     ILTAENLA"
     gene            3376..4365
                     /locus_tag="VIN75_21600"
     CDS             3376..4365
                     /locus_tag="VIN75_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY8976824.1"
                     /translation="MKKFIGFVDDLVPDACAGLAMAHPELLAVNPAPLFVHRRQGKQD
                     RVALVSGGGSGHEPLHVGYVGLGMLDAACPGQVFTSPHPDQIAMAAEHVHAGQGVLLI
                     AKSYAGDLLNFEKAAEMLSIEHAMIVVDDDVTVGNGPPAAGRRGIAGTLVVEKMVGAA
                     AERGDDLRRCQAIGQRVNGCTASMGVAFTACVVPEAAAPTFEIGDSEMEVGVGIHGEP
                     GRYRRDILPAEDIVELLLEGILRDLHPARGQEVLLHVNGFGATPMIELYLLNKLATQA
                     LRDRGLAVVRWLVGNHTTSLDTAGASLTVTLLDDELKALWDAPVHTGSLRWGA"
     gene            complement(4381..5961)
                     /locus_tag="VIN75_21605"
     CDS             complement(4381..5961)
                     /locus_tag="VIN75_21605"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8976825.1"
                     /translation="MFKKLRIGTRLALGFASVLVLTVLAVAVGLLEVAAVQDANQRLL
                     SEPLTKERIASDWYRIIDAGSKRTLAMAVSADPVLPATFADDMKTSTALSTRYQDELT
                     RRAATPEEQARLADILAARRTYLGVRNQIAQLRSDGKGAEADAAAARLKPATGAYLAA
                     LQALLDTERSTIDRSGHAISAEVERSLWLLGALGLAGVLLGIAVCCIVTRGITRPIDR
                     AIGAVRRVAAGDVAHDVAIDRHDELGALQASMADMSSELRGMIAAIRESAAIIAGASG
                     EIANGNADLSQRTESQAAALQRTAGSVDQLSAAVRTTADSSSEALALVNAATCSASEG
                     DTIVATVVTTMREIIESSERIREIVGVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VAAEVRMLAQRSSAAAREVRELIEASTSAVDSGAGQVTRAGAAMRAIRESVGHVDAMI
                     RLIASANGEQADGVDDVNRAVLCLSESTERNAALAEQSAAAAASLRAQSDRLLHAVSA
                     FRIQQAPDFVEEPRPRLQ"
     gene            complement(5998..6702)
                     /locus_tag="VIN75_21610"
     CDS             complement(5998..6702)
                     /locus_tag="VIN75_21610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8976826.1"
                     /translation="MEQAPAQDLSPAQQRWLATLGHEMRTPLNAIIGLASLMIEQRQG
                     GQDAELVRHLGIVKCCGQHLLALGNDLLDSGRLGAGELTLDPQPLDLDDLLREVHGTL
                     QPLATSKGLAFAAPPAANAQLLHADRRALRQIMLNLGGNAIKYTERGAIELQASADDG
                     AVRLGVRDTGVGIAGADLQRLFRPFTQLRERADGAGLGLHLCARIARLMGAHIEVCSE
                     PGVGSVFTLVMPRAAA"
     gene            complement(7106..8437)
                     /locus_tag="VIN75_21615"
     CDS             complement(7106..8437)
                     /locus_tag="VIN75_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8976827.1"
                     /translation="MSTPDRPCVLLIEDDAGVRQAAAQALQIGGFEVVAHESAEQALA
                     DLSATAFPGIVISDVRLPGRDGLTVLRDLRRSDPELPVLLVTGHGDVAMAVEAMRDGA
                     WDFIEKPFASERLIEAARRASAQRQLVVENRRLKAALAGQAASTLIGQTPAIERVRTL
                     IATLGPARVDVMIDGETGCGKEVVARALHDASGASGPFVAINCGALPETVFEAEIFGA
                     EAGAYTGASKRRIGKLEYANDGTVFLDEIESMPMALQVKMLRVLQERMVERLGSNVAV
                     PLQCRIVAATKVDLRKLAEKGTFRDDLYYRLNVATIELPALRDRPDDIPLLLTHFTEM
                     AALRLRRPIPAWSAAQMAEWKLRAWPGNVRELRNFADRWVLGLEQPMPAATPTPAGLI
                     EQVDAFERALIDDALRKAAGSISRTADALGLPRKTLHDKIKRLGMDPDAYR"
     gene            complement(8455..>9250)
                     /locus_tag="VIN75_21620"
     CDS             complement(8455..>9250)
                     /locus_tag="VIN75_21620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8976828.1"
                     /translation="LQDRVDELHRTEQALRATQDDLVQAGKLAVLGQMAASITHEINQ
                     PLAALRALNDNALMLLDRGDDAAVHGNLESIRELTQRIATIVGQLKGFARKDDIRHSP
                     VLVEPAIASAMAMVSADARRHGVEIVAHPMPADVAVRGQAVRVEQILVNLLRNAIDAC
                     AECDTRRVDVHARREGASVNLVVLDSGAGIKPEARDRLFEPFFTTKPAGVGLGLGLAI
                     SGSIANALGGSLQAANREEGGAEFTLRLPAAHWRAEEARATQRTIG"
BASE COUNT         1304 a         3232 c         3269 g         1445 t
ORIGIN      
        1 gggatggagc gcaggccggc cagcagcagc agcatcacgt agggcgtcca catccaggtg
       61 tcgaccagga cgatggccca cggcgcgagg ctcgagtcgg ccagcatcga caggtcctcg
      121 ggcgcgccga acagccgcag cgcctggttg aagatcccgt actggggttg gaagaagtac
      181 ttccagaacg tacccacgac ggcgggcgcg agcatcatcg gcagcaggat cgccgtgctc
      241 cagaagccgt gggtgcggaa gcgccggttg agcatcagcg caaggccgaa cccgagcagc
      301 agctgcagcg tgatcgagca cacgaggaag cgcgcggtgg cctgcaggtg ggcccagatc
      361 tcctcgttgt cgaggacgcg gcggtagttc tcgatgccgg tccacacgag gtcggcgcca
      421 gggtggttgg cccggtagtt cgtgagcgac agcaggatcg tccagaacag cgggaagatg
      481 ttgaacgcga gcagcaggac gatggtgggc gtgacgaaca gccacgccat gccgcggtcc
      541 gagagtccgc gccgttgcca ttgaagggtt tgcatgatgg gaacctgagg aaaagggcag
      601 gaggcgggcc ccgcgtgcgg tgtttgggat ccgcacgcgg cggcgcaggg cggcgaccgg
      661 aggagccgcc gcggccccga ccgggccgcg cgtccgcaaa ggccgttgcc tacttcagtt
      721 ttccgtcctc gcggaaggtc tcttcccagt ccttctcgag cgaatcgaga gcggcctgcg
      781 gcgtcccctt gcccgcgacc acgtagtcat gaacgcgcga ctgcatggcc tcgagcagcg
      841 ccgcatagct cggctcggcc cagaagtcct tgaccatctt catcgagtcg aggaacacct
      901 tcgcgtacgg cgtgctcttg tcgaagccgg gcgcgttcag caccgagctc aggcacgtga
      961 agccgcccgc gtcccaccac ttcttctgca cggccggctg ggcgaaccac ttcatgtact
     1021 ccagcgccat gtccttcttg tccgagtact tcaccaccga gatgccctgc ccgcccagca
     1081 tcgcgaactg cttgtcgccg cgcgggatcg ggaagaatcc ggtcttgtcg ccgccgacct
     1141 gcgggtcctt ggcgatgccg gggaagaagg cgatgaactg catctgcatc gcgacctggc
     1201 cggacttgta ggcgtccagg ttctcggtca tgtacgcgtt cgagtggccc ggcggcgtgc
     1261 agcagtcgta cagctccttg tagaactgca ggcccttgac ggcgccggcg ctgttgaccc
     1321 agcccttcat gtcgtacggc ttcttcgggt tggagtactc gaacccgtag gcgtacatcg
     1381 tgttggtcac gcccatcgtg atgccttcgg agccgcgctc ggtgtagagc gccgcgccgt
     1441 agaccttctt gccgtcgatc acgcggccct ggaagaactc gccgatctgc ttgaactcgg
     1501 gccaggtggc cggcggcgcc aggtcatggc cgtacttctt cttgaactcg gcctgcagct
     1561 cgggacgcgc gaaccagtcc ttgcggtagc tgaaggcgac ggcgtcgccc atggccggca
     1621 gggcccagta gttgggcgtg cccttgggcc actccgagta gcccagcacc gtggcgggca
     1681 tgaagtcggc catcttgatg ccgttcttgt cgaagaagtc gttgagcttg acgtagtggc
     1741 cctcggtggc ggcgccgccg atccactggc tgtcgccgat cagcaggtcg cagagctttc
     1801 ccttggagtt cagctcgttg agcatgcgct gcgcgtagtt cggccagggc acgaactcgt
     1861 acttcatctg gatgccggtc ttggcggtga agtccttcga gagctcgacc agggcgttgg
     1921 cgggatccca ggccgcccag cacagcgtga gttgcttctg gtcggcggct tgcgcgcccg
     1981 tgcacatcgc tgtgcccagg aacgcggcga tagccgcggc ggcggccttc ttcgtggtgg
     2041 tcatcagtct gtctcctgtt gatcggaagg ggcggttcgg cgaccgctga tggccaatgt
     2101 atcgcaatca aaaaataaat caagcaaaaa agacttcaac atcatggtca gttttcacga
     2161 tcgaatccgg cagggaagcc agggggcggg ccagctcgac ggtacgcagg ccgcggaacg
     2221 aagtccctgg ggtagcggca gggaacccgg atgggtgaca atgcgatgta tttacttgtc
     2281 cgatcgatcc agccgcccca tggcgaatcc actcaccatc gcccagatcg ccgaggccgc
     2341 cggtgtcagc acggcgacgg tcgaccgcgt gctcaacgag cgcgccggcg tgaatccggc
     2401 cacggtcggc aaggtgcgcg aggccatcgc ggcgctcggg ggcggcacgc ccgcgcgcgg
     2461 gcgtccgcgc tcgagcagca actaccgctt cggcttcgtc ctgccatcgg cgcggcgcgg
     2521 cttcttcgac caggtcgacc gcgtcatcgc gcagacggcc ggcgagttcc ggcaccagca
     2581 catcaccgag gtgacccatc gcctgcccgc ggccgacgtg aaccagttcg ccgcggagct
     2641 caccaagctg tcggacctgg acggcctggc gctcatcgcg cccgacctgc cccccgtcaa
     2701 gctggcgatc aacgaactcg tgcgcgcggg cgtccacgtc gtcacgctgt tctccgacgt
     2761 gcccggctcc atgcgcgcga cggccatcgg cgccgacaat cgcgccgcag gccgcaccgc
     2821 aggcctgctg atcggcaact cgctgccgcg cgacgccggg cgcacctgcg ccttgctgtc
     2881 gccggccacg cgctatgccg cggagatcga tcgccgcatc ggcttccagc aggtgatgga
     2941 ggagcgcttc cccgatgccg tggtcacgcg cctgttcgag atgcccgaag ccgacgatgc
     3001 cgcctacgag atggcgcgcg agctgctcac gcccgtggcg gccgacctgg ccggcgtcta
     3061 caacgtcggg cccgcctcgt tcggcgtcgc gcgggcgctg gcggagatga agaccacccg
     3121 accgccgatg ttcgtcgcca acgacctgct ggaggtgcat cgcaccatgc tgatgtccgg
     3181 cgccctgagc tacgtgatca accaggatct gtacagctcg atcagcaacg ccagccgcgt
     3241 gctgcgcggc ctgtgcgacg gcgtgcgcgg cgcgctgtcg ctgggcaacc ctcgcgtgga
     3301 gatcctcacc gcagagaacc tcgcgtgaca tctccaaact cgtgcgccag aatggtgctt
     3361 ccagcagaga gcaccatgaa gaagttcatc gggttcgtcg acgatttggt gccggacgcc
     3421 tgcgcgggtc tcgcgatggc gcatcccgag ctcctggccg tgaatccggc gcccctgttc
     3481 gtgcaccgcc gccagggcaa gcaggatcgc gtcgcgctcg tgagcggcgg cggttccggc
     3541 cacgagccgc tgcacgtcgg ctacgtcggc ctcgggatgc tcgatgcggc ctgccccggg
     3601 caggtgttca cctcgccgca tcccgaccag atcgccatgg ccgccgagca cgtgcatgcg
     3661 ggccagggcg tgctgctgat cgccaagagc tatgccggcg acctgctcaa tttcgagaag
     3721 gccgccgaga tgctctcgat cgagcacgcg atgatcgtcg tcgacgacga cgtcaccgtg
     3781 ggcaacggcc cgccggccgc gggccgccgg ggcatcgcgg gcacgctggt cgtcgagaag
     3841 atggtcggcg ccgccgccga gcgcggcgac gacctgcgcc gctgccaggc catcggccag
     3901 cgcgtgaacg gctgcaccgc ctccatgggc gtcgccttca cggcctgcgt cgtgccggag
     3961 gcggccgcgc ccaccttcga gatcggcgac agcgagatgg aggtcggcgt cggcatccac
     4021 ggcgagccgg ggcgctatcg ccgcgacatc ctgcccgccg aggacatcgt cgaactgctc
     4081 ctggagggca tcctgcgcga cctgcatccg gcccgcggcc aggaggtgct cctgcacgtc
     4141 aacggcttcg gcgcgacgcc gatgatcgag ctctacctgc tcaacaagct cgcgacccag
     4201 gcgctgcgcg atcgcgggct ggcggtcgtg cgctggctgg tcggcaacca caccacctcg
     4261 ctggacaccg ccggcgcctc gctgaccgtc acgctcctgg acgacgagct caaggcgctg
     4321 tgggacgccc cggtccacac gggttccctg cgctggggtg cctgaggccg cgccagcggc
     4381 tcattgcagc cggggacgcg gttcctccac gaagtccggc gcctgctgga tccggaacgc
     4441 cgagaccgcg tgcagcagcc ggtcggactg cgcgcgcagc gacgccgccg cggcggccga
     4501 ctgctcggcg agcgcggcat tgcgttccgt cgactcgctc aggcacagca ccgcccggtt
     4561 gacgtcgtcg acgccgtccg cctgctcccc gttggcgctc gcgatcagcc ggatcatggc
     4621 gtcgacgtgg ccgaccgatt cgcggatggc ccgcatcgcc gcaccggcgc gcgtcacctg
     4681 gcccgcgccg gagtcgacgg cgctcgtcga cgcctcgatc agttcgcgca cctcgcgcgc
     4741 ggcggcgctg ctgcgctggg ccagcatccg cacttcggcg gccacgacgg cgaagccgcg
     4801 cccctgctcg ccggcgcgcg cggcctcgac cgccgcattg agcgcgagga tgttggtctg
     4861 gaaagcgatg ccgtcgatga cgcccacgat ctcgcggatg cgctcggacg actcgatgat
     4921 ttcgcgcatg gtcgtgacca cggtcgcgac gatcgtgtcg ccttcgctcg ccgagcaggt
     4981 ggcggcgttg acgagggcca gcgcctcgga gctgctgtcg gccgtcgtgc gcacggcagc
     5041 gctcagctgg tcgacgctgc ccgcggtgcg ctgcagcgcg gccgcctggc tctccgtgcg
     5101 ctgcgacagg tccgcgttgc cgttggcgat ctcgcccgag gcgccggcga tgatcgcggc
     5161 gctctcgcgg atggccgcga tcatgccgcg cagctcgctg gacatgtcgg ccatcgaggc
     5221 ctgcagcgcg ccgagctcgt cgtggcggtc gatcgcgacg tcgtgcgcca cgtcgcccgc
     5281 ggccacgcgc cgcacggcgc cgatcgcgcg gtcgatgggc cgggtgatgc cgcgcgtgac
     5341 gatgcagcac acggcgatgc ccagcagcac gccggccagg ccgagggcgc cgagcagcca
     5401 caggctgcgc tcgacctccg cgctgatcgc gtggccgctg cggtcgatgg tcgagcgctc
     5461 ggtgtccagc aacgcctgca gcgccgcgag gtaggcgccg gtggctggtt tcaggcgcgc
     5521 cgcggccgcg tcggcctcgg cgcccttgcc gtcgctgcgc agctgcgcga tctggttgcg
     5581 cacgcccagg tacgtcctgc gcgcggccag gatgtcggcc aggcgcgcct gctcctcggg
     5641 cgtggccgca cgcctggtca actcgtcctg gtaccgggtc gacagcgccg tcgacgtctt
     5701 catgtcgtcg gcgaacgtgg cgggcagcac gggatcggcg ctcacggcca tcgccagcgt
     5761 ccgcttgctc ccggcgtcga tgatgcgata ccagtcgctg gcgatgcgct ccttggtcag
     5821 gggctcggac agcaggcgct ggttggcgtc ctgcaccgcg gcgacctcca gcaggccgac
     5881 ggccacggcc agcacggtca gcacgaggac gctggcgaag cccagcgcga gccgggtgcc
     5941 gatgcgaagc ttcttgaaca tggggtcgct ctccgtttcg tggggatggc gccggcctca
     6001 ggcggccgcg cgcggcatga cgagcgtgaa gacgctgccg acgccgggct cgctgcagac
     6061 ctcgatgtgc gcgcccatca gccgggcgat ccgcgcgcac aggtgcagtc ccaggccggc
     6121 gccgtcggcg cgctcgcgca gctgcgtgaa ggggcggaac aggcgctgca ggtcggcacc
     6181 cgcgatcccg acccccgtgt cgcggacgcc cagccgcacg gcgccgtcgt ccgcgctcgc
     6241 ctgcagctcg atcgcgccgc gctcggtgta cttgatcgcg ttgccgccga ggttgagcat
     6301 gatctggcgc agcgcgcgcc ggtcggcgtg caggagctgg gcgttcgcgg cgggcggcgc
     6361 ggcgaatgcc aggcccttgc tcgtggccag cggctgcagc gtgccgtgca cctcgcgcag
     6421 caggtcgtcc aggtccagcg gctgcgggtc cagcgtgagc tcgccggcgc ccaggcgtcc
     6481 ggagtcgagc aggtcgttgc ccagggccag caggtgctgc ccgcagcatt tgacgatgcc
     6541 caggtggcgc acgagctcgg cgtcctggcc gccctggcgc tgctcgatca tcagggatgc
     6601 gaggccgatg atggcgttga gcggcgtgcg catctcgtgg cccagcgtgg cgagccagcg
     6661 ctgctgcgcc ggcgacagat cctgcgccgg agcctgctcc atgacttcgg tccgcgcctg
     6721 gcgggcttct cgcgacgatg tcagaacttc catgtcgacc tccaatgtcc acatcacgga
     6781 tggcattgcg ccatgccggg gtcgatgcga aagtcgtgcc agtggggttc gtcgccgctc
     6841 tccgggggag cgcgtgggct cacgcacgaa agttggcgga agtccgccga agcgctggcg
     6901 aaaagccgcc gcgcgtcgag atgggctcgt gccggggtgg atcctgcgac tccgcttcgc
     6961 cgcgcgcagc gatgacggcg cgggcgcatc gcgtgacgca ggaccagggg gtcggcgcgt
     7021 ggcctgacgc accatcgccg catcgcgcac accaagtgtc atcctgcggg cagtcgcagg
     7081 atccacgcgg gcgcgcccag cccgcctagc gataggcgtc cggatccatg ccgagccgct
     7141 tgatcttgtc gtgcagcgtc ttgcgcggca ggccgagcgc gtcggccgtg cgcgagatgc
     7201 tgccggcggc cttgcgcagc gcatcgtcga tcagcgcgcg ttcgaaggcg tcgacctgct
     7261 cgatgagtcc ggccggcgtg ggcgtcgcgg cgggcatcgg ctgctccagg cccagcaccc
     7321 agcggtccgc gaagttgcgc agctcgcgca cgttgccggg ccacgcgcgc agcttccatt
     7381 cggccatctg cgccgcggac cacgccggga tcggccggcg cagccgcagc gcggccatct
     7441 cggtgaagtg cgtcagcagc aaggggatgt cgtcgggccg gtcgcgcagc gcgggcagct
     7501 cgatggtggc cacgttgagg cggtagtaga ggtcgtcgcg gaacgtgccc ttctcggcga
     7561 gcttgcgcag gtcgaccttg gtggccgcca cgatgcggca ctgcagcggc acggccacgt
     7621 tcgagcccag gcgttcgacc atccgctcct gcagcacgcg cagcatcttg acctgcagcg
     7681 ccatcggcat cgactcgatc tcgtcgagga acaccgtccc gtcgttggcg tattcgagct
     7741 tgccgatgcg ccgcttgctg gcgccggtgt aggcgccggc ctcggcgccg aagatctcgg
     7801 cctcgaacac cgtctcgggc agcgcgccgc agttgatcgc cacgaacggg ccgctcgcgc
     7861 cgctggcgtc gtgcagggcg cgggcgacga cttccttgcc gcagccggtc tcgccgtcga
     7921 tcatcacgtc aacgcgtgcc gggcccaacg tggcgatcag cgtgcgcacg cgctcgatgg
     7981 cgggcgtctg cccgatgagc gtgctcgcgg cctggcccgc gagcgcggcc ttcaggcggc
     8041 ggttctcgac gaccagctgg cgctgcgccg aggcgcgccg cgcggcctcg atgaggcgct
     8101 cggacgcgaa cggcttctcg atgaagtccc acgccccgtc gcgcatcgcc tccaccgcca
     8161 tcgcgacgtc gccgtggccc gtgaccagca gcaccggcag ctcgggatcg gagcggcgca
     8221 ggtcccgcag cacggtcagg ccgtcgcgcc cgggcaggcg cacgtcgctg atcacgatgc
     8281 ccgggaacgc cgtcgcggac aggtccgcga gcgcctgctc cgcggactcg tgcgcgacga
     8341 cctcgaagcc gccgatctgc aacgcctgcg ccgccgcctg gcgcacgccg gcgtcgtcct
     8401 cgatcagcag gacgcaggga cggtctggcg tgctcatggg gcctctcctg gttcttatcc
     8461 gatggtgcgc tgcgtcgcgc gggcttcctc ggcgcgccag tgcgccgcgg gcagccgcag
     8521 cgtgaactcg gcgccgccct cctcgcggtt ggcggcctgc aggctgcccc cgagcgcgtt
     8581 ggcgatggag cccgagatgg cgagccccag gccgaggccc acgcccgcgg gcttggtcgt
     8641 gaagaacggc tcgaacaggc ggtcccgcgc ctccggcttg atgccggctc cgctgtcgag
     8701 caccacgagg ttgacgctgg cgccctcgcg ccgcgcgtgc acgtcgacgc gccgcgtgtc
     8761 gcattcggcg caggcgtcga tggcgttgcg cagcaggttc acgaggatct gctccacgcg
     8821 cacggcctgt cctcgcacgg cgacgtccgc gggcatcgga tgcgcgacga tctcgacgcc
     8881 gtgccggcgg gcatcggccg acaccatggc catggccgac gcgatggccg gttcgaccag
     8941 caccggcgaa tggcggatgt cgtccttgcg cgcgaagccc ttgagctgcc cgacgatcgt
     9001 cgcgatgcgc tgcgtgagct cccggatcga ttcgaggttg ccgtgcacgg cggcatcgtc
     9061 gccgcgatcg agcagcatca gcgcgttgtc gttgagggcg cgcagcgcgg ccagcggctg
     9121 gttgatctcg tgggtgatgc tggccgccat ctggcccagc acggcgagct tgccggcctg
     9181 cacgagatcg tcctgcgtgg cgcgcagcgc ctgctcggtg cggtgaagtt cgtcgacgcg
     9241 gtcctgcagt
//
LOCUS       DATCRO010000793         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_189209, whole genome shotgun sequence.
ACCESSION   DATCRO010000793 DATCRO010000000
VERSION     DATCRO010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189209"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..405
                     /locus_tag="VIN75_21625"
     CDS             178..405
                     /locus_tag="VIN75_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976829.1"
                     /translation="MRLLTSLALITIAYAALTRVNKVHTARSHGKPAAKPEAVQTWEG
                     EGGGLPSGGPGPGVKVAPAAVPDPDLAHGAT"
     gene            complement(409..1704)
                     /locus_tag="VIN75_21630"
     CDS             complement(409..1704)
                     /locus_tag="VIN75_21630"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8976830.1"
                     /translation="MLTATLLLLGAVLLGMCLLELHVTRLPLSPAVVYLAVGWIAGWM
                     VQARVVTPPTAPARADMLVIITEIAVLISLFAVGLQLRMPWTFKGWRVALVLAGGTMV
                     ATIALATLAGAWLMPGLGWAGALLLAGILAPTDPVLASEVQIRSRHDRDAVRVALTAE
                     GGLNDGTALPVVMLALGLLGIGDLGPAGARWLWHDLAWPIAGGALLGWAFGRVLGRVV
                     HALQRRGHGLGWDELMYLGIITVAYGLSRLTETSAFLFVFAAALGLFRRSPQAVASLA
                     RAGVEPDELAERLLGFGHRCGRLVEVVMVLLMGFAMPWVQWRGEPLVYATLLLLVIRP
                     LSVFLTIPGRALPRTQRRIVAWFGIRGVGSLFYLALVIDSGLSPTLATDLMNATLPAI
                     ALSILLHGVSATPLMALYRRRRVLNRRDKRRLARAAAPR"
     gene            complement(1742..>2933)
                     /locus_tag="VIN75_21635"
     CDS             complement(1742..>2933)
                     /locus_tag="VIN75_21635"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY8976831.1"
                     /translation="GVAALNIGSSRLNGNFRFDSGPAVPVLTGKLGGARLALADLGPA
                     FGATPPDAPASASNAKPGKVIPDREFDIPSLKMMNADVRVALATVDLGTARLEPFKPL
                     DGRITLQDGVLDLKDLLARTSAGEVRGAIGLDSRPAVPKWSADLRWSGIQLERFVTAR
                     DVAVHKDAKGEKPDTGYISGALGGNAQLKGQGKSTAQLLASLDGTARLWVRDGTVSHI
                     LVEAAGIDVAQALGMFVKGDDKLPMQCAVAQFDLVNGKVMPEVFVIDTKDTTMLIDGD
                     LSLANETLDLRMTAHPHDFSPLALRTPVKVVGTFDAPHIRPEVKPLAERAGAAVLLGL
                     ANPLASLLALIDFKQKERDVCTAAVSHVPGAAKAGVKTGPAEAPPLPASSPKARFAPP
                     RKGA"
BASE COUNT          445 a         1102 c         1035 g          351 t
ORIGIN      
        1 tgctcacgct gggccccaag gacgactaca tcctcgatat cgacaaggcg cgcctgcagg
       61 ccgcccccgg cttcacctcg aagacctggc ccaacctcgg cgacgagcgc tggctggccg
      121 acaaccgccg cttctacgtc cactgaccca cgacctcagc ccacggggag aaccaccatg
      181 agactgctca cgagcctggc cctcatcacc atcgcctacg cggccctgac ccgcgtcaac
      241 aaggtgcaca ccgcgcgctc ccacggcaag cccgccgcca agccggaggc ggtgcagacc
      301 tgggagggcg agggcggcgg cctgcccagc ggcggccccg gcccgggcgt gaaggtcgcg
      361 ccggccgcgg tgcccgaccc cgacctcgcg cacggggcca cctgagcgtc aacgcggcgc
      421 ggcggcgcgc gcgagccgcc gcttgtcgcg ccggttcagc acccgccggc gccggtacag
      481 cgccatcagc ggcgtggccg acacgccgtg caggaggatc gacagcgcga tcgccggcag
      541 cgtcgcgttc atcaggtcgg tggccagcgt cggcgacagc cccgagtcga tcaccagcgc
      601 caggtagaac agcgagccga cgccgcggat gccgaaccag gcgacgatgc ggcgctgcgt
      661 cctgggcagc gcgcggcccg ggatggtcag gaacacgctc agcggccgga tcacgagcag
      721 cagcagcgtc gcgtagacca gcggctcgcc gcgccactgc acccagggca tcgcgaagcc
      781 catcagcagc accatcacca cctcgacgag gcggccgcag cggtggccga acccgaggag
      841 tcgctcggcc agctcgtcgg gctcgacgcc ggcgcgcgcg agcgatgcca cggcttgcgg
      901 cgaacgccgg aacagcccga gcgcggcggc gaacacgaac aggaaggccg aggtctcggt
      961 caggcgcgac aggccgtagg ccacggtgat gatgcccagg tacatcagct catcccaacc
     1021 caggccgtgg ccgcgccgct gcagcgcgtg gacgacgcgc cccagcacgc gcccgaaggc
     1081 ccatccgagc agcgcgccgc cggcgatcgg ccaggccagg tcgtgccaca gccagcgcgc
     1141 gccggcgggg cccaggtcgc cgatgcccag cagtccgagc gccagcatca ccaccggcag
     1201 cgcggtgccg tcattcaggc cgccctcggc agtcagcgcg acgcgcacgg cgtcgcggtc
     1261 gtggcgcgag cggatctgca cctcggaggc cagcaccggg tcggtgggcg cgaggatgcc
     1321 cgccagcagc agggcgcctg cccagcccag gcccggcatc agccaggcgc cggccagcgt
     1381 ggccagcgcg atcgtggcca ccatcgtgcc gccggccagc accagcgcca cccgccagcc
     1441 cttgaacgtc cacggcatgc gcagttgcag gcccaccgcg aacagcgaga tcagcaccgc
     1501 gatctccgtg atgatcacca gcatgtcggc gcgcgccggc gcggtcggcg gcgtcactac
     1561 ccttgcctgg accatccagc cggcgatcca gccgacggcc aggtagacga cggccggcga
     1621 cagcggcagc cgcgtcacat gcagctccag caggcacatg cccaacagca cggcgccgag
     1681 cagcagcagc gtggcggtca gcatcgcgcg tcccgcgtgc gcgagcaggt gcccatgcgc
     1741 gtcacgcgcc cttgcgcggg ggcgcgaacc tggcctttgg cgaggaggcc ggcaacggcg
     1801 gcgcctcggc cggcccggtc ttcacgccgg ccttggccgc ccccggcacg tgcgacaccg
     1861 ctgcggtgca cacgtcgcgc tccttctgct tgaagtcgat cagcgccagc agcgaggcga
     1921 gcgggttggc cagccccagc aggacggccg cgccggcgcg ctcggccagc ggcttgacct
     1981 cgggccggat gtgcggcgca tcgaacgtgc ccaccacctt gaccggcgtg cgcagcgcca
     2041 gcgggctgaa atcgtgcgga tgcgcggtca tgcgcaggtc cagcgtctcg ttggccagcg
     2101 acaggtcgcc gtcgatcagc atcgtcgtgt ccttggtgtc gatgacgaac acctcaggca
     2161 tcaccttgcc gttgaccagg tcgaattgcg cgacggcgca ttgcatcggc agcttgtcgt
     2221 cgcccttgac gaacatgccc agggcctgcg cgacgtcgat gcccgcggct tccaccagga
     2281 tgtgcgacac cgtgccgtcg cgcacccaca ggcgggcggt gccgtccagc gacgcgagga
     2341 gctgcgccgt cgacttgccc tggcccttga gctgcgcgtt gccgcccagt gccccgctga
     2401 tgtagccggt gtcgggcttc tcgcccttgg cgtccttgtg cacggcgacg tcgcgcgccg
     2461 tcacgaagcg ttcgagctgg atgccgctcc agcgcaggtc ggcgctccac ttgggcacgg
     2521 cggggcggct gtccaggccg atggcgccgc gcacctcgcc ggcggaggtg cgcgcgagca
     2581 ggtccttcag gtcgagaacg ccgtcctgca gcgtgatgcg cccgtccagc ggcttgaagg
     2641 gctcgagccg ggcggtgccc aggtcgaccg tcgccagcgc cacgcgcacg tcggcgttca
     2701 tcatcttcag cgacgggatg tcgaactcgc gatcggggat gaccttgccg ggcttggcgt
     2761 tggaggcgga tgccggcgcg tcgggcggcg tggcgccgaa ggccgggccg aggtcggcca
     2821 gcgccaggcg cgcgccgccc agcttgccgg tcagcacggg caccgcgggg ccgctgtcga
     2881 aacggaaatt gccgttcagg cggctgctgc cgatgttcag cgccgccacg ccg
//
LOCUS       DATCRO010000794         6137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1044186, whole genome shotgun sequence.
ACCESSION   DATCRO010000794 DATCRO010000000
VERSION     DATCRO010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044186"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VIN75_21640"
     CDS             <1..704
                     /locus_tag="VIN75_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976832.1"
                     /translation="RRPGIPVACGASGVARRQLTAASVGLGNFCPASRLPPAARVDDM
                     GRGGEPRHTRRAMTHRASAPRRGGCFLDVPQGGGIDSNTLNPSCEVRIMKTSRSAIAV
                     ALLCLSAGATAADYVYLLPLGTPLNESVRYGFDDTVTFTPPESGVYNVDVVASTVYGG
                     CSTRYCHSKWSIVTTITSETLTDGSSTWPLVQGNVTLPMVAGVPYVLHLSGVGTGTGV
                     YAGYGAYSVSIAQLQ"
     gene            complement(764..1912)
                     /locus_tag="VIN75_21645"
     CDS             complement(764..1912)
                     /locus_tag="VIN75_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8976833.1"
                     /translation="MLQHLRLFALAALAVTAASAGATDLHRAHDAAPPKVILDTDFNT
                     ISDDGQALAMLAQLDAAGKLDLLGVAVATGNAWLEQETSDALKAVERLGIERQIGVYV
                     GAKYGLLHDYAAYQDEVATFGPAVDYVGAFSSPPPTSPSDLVPPPDGFATHTLPQRQH
                     AVDFMIEQIHRYPHQVSILEIAPPTNLAMAIRKDPGIVPLIKQVVTMAGQIYVPGNAY
                     NDAAEFNWWADPEAVKIVLRAPIPKVIVPLDLTNNVPLTRPVYQQIANHQPATPITKL
                     FADAYAPFFGSAPPPYPLFIWDTTALAYLVDPTFATATRDLWVDMNTTFDANYGKSIV
                     YDANPKPAIPLLTQSKVVLAFDTARFYAFYADLLTRPVPVHLDHGHEH"
     gene            complement(2057..3109)
                     /locus_tag="VIN75_21650"
     CDS             complement(2057..3109)
                     /locus_tag="VIN75_21650"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8976834.1"
                     /translation="MSSFARWLILVVAAAGMVAGRPSRAADAPLPVEPLQPSLAGATA
                     WLNSPPLTLEQLRGKVVLVDFWTYSCINCLRTLPAVRAWARTYAGRGLVVVGVHAPEF
                     EFEHDPQRVRQALVDQHVDWPVAIDDEFRVWRAFRNEAWPALYFIDAQGRVRHHVLGE
                     GGYDESERVIRQLLAEARGDAAPLPAMAAVEATGIGAAPDLAHVRSGETYLGYAHADA
                     ATPPDVLPERLQQHAPRAPRLNAWTLGGGWVQRGEFVESATPGATVALRFHARDLHLV
                     LGPGADGSPVAFRVTIDGHAPGADHGVDTDEQGRGLVTHERLYQLVRQRGAVADRTFE
                     IHLDGAGARAWVFTFG"
     gene            complement(3129..3329)
                     /locus_tag="VIN75_21655"
     CDS             complement(3129..3329)
                     /locus_tag="VIN75_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976835.1"
                     /translation="MSRFVPRLPSWFVVATRDHMIAPELQLAIAQRIGAQVTKLPTSQ
                     VSQEVVPDKVAAVILHAVAHAR"
     gene            complement(3365..3781)
                     /locus_tag="VIN75_21660"
     CDS             complement(3365..3781)
                     /locus_tag="VIN75_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY8976836.1"
                     /translation="MTSIDKVLYTGQTHTIGGRDGAARSSDGKLDIRLSPPGSNGPGT
                     NPEQLFAAGWSACFIGAMGRAARASGVALPADVAVDAEVDLGQAGDAFLLQARLKVSL
                     PGLDAAVARAIVDGAHQLCPYSKATRGNIAVELSLA"
     gene            3948..4382
                     /locus_tag="VIN75_21665"
     CDS             3948..4382
                     /locus_tag="VIN75_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8976837.1"
                     /translation="MPRLGDFLCFAVYSANLAYGRAYKPILDELGLTYTQWITIVALW
                     EHDEQTVSSLGDKLFLESNTLTPILKKLEALGYLERRRDARDERQVIVRLTDAGRALR
                     EKGAQRTLVQATGLDAGQFAAARRTMNEVRDNLLRHTRQAGD"
     gene            complement(4399..5091)
                     /locus_tag="VIN75_21670"
     CDS             complement(4399..5091)
                     /locus_tag="VIN75_21670"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8976838.1"
                     /translation="MNRSFQSERDRMVERQLMGRGIRDEHVLRAMRTVPRERFVDERM
                     AAFAYDDSPLPIAAEQTISQPFMVAWMIEAAEVVPGDHVLEVGAGSGYASAVLAQVAA
                     TVHAIERHELLTRTARERLDALAYANIHLRTGDGTLGWPEAAPFDAILVAAAGPEVPA
                     PLKAQLRVGGRLLMPLDIGGHGTQRLLKVVRHAQDDYEQQTLGSVAFVPLIGQYGWTE
                     GDERAGAHATRP"
     gene            5288..>6137
                     /locus_tag="VIN75_21675"
     CDS             5288..>6137
                     /locus_tag="VIN75_21675"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY8976839.1"
                     /translation="MKTLLRHLRIGHRLVLCFSLILLLMIGGAWLAVSSSRAARESLV
                     HLVAVSNARQADIRAMRSLLERQDRVSQRLGLTNSIEDARNDMQTIVEDIAAYRVVER
                     RYASHVDSVLERSMVEQIEHYDRTLAAAFAQAQDSVAAYNPGMAARTLNRDVAPIHAA
                     WLHLLDDLAELQNQRVAAEIQALSAHANRNDLVIGAVALLASLLAALAGWRLTLSITR
                     PLRQAVLFASAVGSGQLDAPLPRSSDDECGMLLLALKAMARQLQEANARMQRLGLTNS
                     IEDARND"
BASE COUNT          881 a         2101 c         2236 g          919 t
ORIGIN      
        1 cgcgtcgacc gggcattccc gtcgcgtgcg gggcatccgg cgtcgcgcgc cgacagctaa
       61 ccgccgcaag cgtcggactc ggcaatttct gcccggcatc ccgattgccg cccgccgctc
      121 gagttgacga catgggcagg ggcggcgagc cacgccacac caggcgcgcg atgacgcatc
      181 gtgcatcggc gccgcggcgg ggcggctgtt tcctcgacgt gccgcaagga gggggaatcg
      241 attccaacac cctcaatcct tcttgcgagg tacgcatcat gaagacgtcc cgatccgcga
      301 tcgcagtcgc cctcctgtgt ctttccgcag gcgcaaccgc cgccgattac gtgtatttgt
      361 tgccgctcgg aacgccgctc aatgaatcgg ttcgttatgg cttcgatgat acggtcacgt
      421 tcacgccgcc cgagtcgggt gtctacaacg tcgatgtcgt ggcgtcgacc gtgtacggtg
      481 gctgcagcac gcggtactgc cattcgaagt ggtccatcgt gaccacgatc acgtcggaga
      541 cgctgaccga cggttccagc acctggcccc tggtgcaggg caacgtgacg ctgccgatgg
      601 tcgcgggcgt cccttacgtg ctgcacctgt ccggcgtggg gaccgggacg ggcgtctatg
      661 cgggctacgg cgcctacagc gtgtcgatcg cgcagttgca gtgacgccgt gactcggcgg
      721 ggggccctcc gccggccgcc ctggccgggc ggaggccgcc gcgtcagtgc tcgtgcccat
      781 ggtccaggtg caccggcacg ggccgggtca gcaggtcggc gtagaacgcg tagaagcgcg
      841 ccgtgtcgaa ggcgagcacc accttggact gcgtcagcag cgggatcgcc ggcttggggt
      901 tcgcgtcgta gacgatggac ttgccgtagt tggcgtcgaa cgtcgtgttc atgtcgaccc
      961 acaggtcgcg cgtggcggtg gcgaaggtcg ggtcgaccag gtaggcgagc gcggtggtgt
     1021 cccagatgaa cagcgggtag ggcggcggcg ccgagccgaa gaacggcgcg taggcgtcgg
     1081 cgaacagctt cgtgatgggc gtggccggct ggtggttggc gatctgctgg tagaccggcc
     1141 tggtcagcgg cacgttgttg gtcaggtcca gcggcacgat caccttgggg atgggggcgc
     1201 gcagcacgat cttgaccgcc tccggatcgg cccaccagtt gaactccgcg gcgtcgttgt
     1261 aggcgttgcc gggcacgtag atctggccgg ccatcgtcac cacctgcttg atcagcggca
     1321 cgatgcccgg atccttgcgg atggccatcg cgaggttggt cggcggcgcg atctccagga
     1381 tcgatacctg gtgggggtac cgatggatct gctcgatcat gaagtcgacc gcatgctggc
     1441 gctgcggcag cgtgtgcgtg gcgaatccgt cgggcggcgg cacgagatcg ctcggcgagg
     1501 tcgggggcgg gctggagaag gcgccgacgt agtcgaccgc ggggccgaac gtggccacct
     1561 cgtcctggta cgccgcgtag tcgtgcagca ggccgtactt tgcgccgacg tacacgccga
     1621 tctgccgctc gatgcccagg cgctcgacgg ccttgagcgc gtcggaggtc tcctgctcga
     1681 gccacgcatt gccggtggcg accgcgacgc ccagcaggtc gagcttgccg gccgcgtcga
     1741 gctgcgcgag catcgccagc gcctggccgt cgtcgctgat ggtgttgaag tcggtgtcca
     1801 ggatcacctt gggcggcgcc gcgtcgtgcg cacgatggag gtccgtggcg cctgcggacg
     1861 cggccgtgac ggccagcgca gcgagcgcga acaggcgaag gtgttgcagc atgcgggtct
     1921 tcctgtcgtg tgcggcgagc gtgggtgccg cgtcccgggc catcatcgcg ctggaggatg
     1981 ccgcggtccg accagacttg cccgggggtc gttggccaac cccacgatag ccatacgggt
     2041 atgcgtatgg tcgagctcag ccgaacgtga acacccaggc ccgcgcgccg gcgccgtcga
     2101 ggtggatctc gaacgtgcgg tccgccacgg cgccgcgctg gcgcacgagc tggtagagcc
     2161 gctcgtgcgt gaccaggccg cggccttgct cgtccgtgtc gacgccgtgg tcggcgcccg
     2221 gcgcgtggcc gtcgatggtg acgcggaagg ccaccgggct gccgtccgcg cccgggccga
     2281 gcaccaggtg caggtcgcgc gcatggaagc gcagcgcgac cgtggcgccg ggcgtggccg
     2341 actcgacgaa ctcgccgcgt tggacccagc cgccgcccag cgtccacgcg ttcaggcgcg
     2401 gcgcgcgcgg ggcgtgctgc tgcaggcgct cgggcagcac gtcgggcggc gtcgcggcgt
     2461 cggcgtgcgc gtagccgagg taggtctcgc ccgagcgcac gtgcgcgagg tccggcgctg
     2521 cgccgatgcc tgtcgcctcg acggcggcca tcgcgggcag cggcgccgcg tcgccgcggg
     2581 cctcggcgag cagttgcctg atcacgcgct cggactcgtc gtagccgcct tcgcccagca
     2641 cgtggtgccg cacgcgcccc tgcgcgtcga tgaagtacag cgccggccag gcctcgttgc
     2701 ggaaggcgcg ccagaccctg aattcatcgt cgatggcgac gggccaatcg acgtgctggt
     2761 cgaccagcgc ctggcgcacg cgctggggat cgtgctcgaa ctcgaactcc ggcgcgtgca
     2821 cgcccaccac gaccagcccc cggcccgcgt acgtgcgggc ccatgcgcgc acggccggca
     2881 gcgtgcgcag gcagttgatg caggagtagg tccagaagtc gaccagcacc accttgccgc
     2941 gcaactgctc gagcgtcagc ggcggcgaat tgagccaggc ggtggcgccg gcgaggctgg
     3001 gctggagcgg ctcgacgggc agcggcgcgt cggcggcccg cgaggggcgg ccggcgacca
     3061 tgcctgccgc ggcgacgacg aggatcagcc agcgtgcgaa cgaggacatg gcgcggctgc
     3121 cctgcgtgtc agcgcgcgtg cgcgacggcg tgcaggatca ccgccgccac cttgtccggc
     3181 acgacttcct gcgacacctg gctggtgggc agcttcgtga cctgcgcgcc gatgcgctgc
     3241 gcgatcgcga gctgcagttc cggcgcgatc atgtggtcgc gcgtggccac gacgaaccag
     3301 ctgggaaggc gggggacgaa gcgggacatg gagttcctcg gggtgtcggg tgtacgggag
     3361 ggactcaggc gagcgacagt tcgacggcga tgttgccgcg cgtcgccttc gagtaggggc
     3421 acagctggtg cgcgccgtcg acgatcgccc gcgcgaccgc cgcatcgagg ccgggcaggc
     3481 tcaccttcag gcgcgcctgc agcaggaagg cgtcgccggc ctggcccagg tcgacctcgg
     3541 cgtccaccgc cacgtcggcc ggcagcgcga cgcccgacgc gcgcgcggcg cggcccatgg
     3601 cgccgatgaa gcacgccgac cagccggccg cgaacaactg ctcggggttc gtcccggggc
     3661 cgttgctgcc cggcggggac aggcggatgt ccagcttgcc gtcgctgctg cgggcggcgc
     3721 cgtcgcggcc gccgatggtg tgggtctgcc ccgtgtagag gactttgtcg atggaggtca
     3781 tggtcgtttc ctggttgaac atcggatgcg agtaaatcgc atgcgatcaa tgtagtaggg
     3841 tgtcttcgcg atgtcaactg atgcgatcaa atcggatgcg atttgatcgg cgtatgatgc
     3901 gagccaagga gacgatgccc gtgaccaagc ccgccacgcc cccccagatg cccaggctgg
     3961 gcgacttcct gtgcttcgcc gtctacagcg cgaacctggc ctacggccgc gcgtacaagc
     4021 ccatcctcga tgagctgggc ctgacctata cccaatggat cacgatcgtc gcgctgtggg
     4081 agcacgacga gcagaccgtc agcagcctgg gcgacaagct gttcctcgag tcgaacacgc
     4141 tcacgcccat cctgaagaag ctggaggcgc tgggctacct ggagcgccgg cgcgacgcgc
     4201 gcgacgaacg ccaggtgatc gtcaggctca ccgacgccgg acgggcgctg cgcgagaagg
     4261 gcgcgcagcg caccctggtg caggccacgg gcctggacgc cggccagttc gccgcggcgc
     4321 gccgcacgat gaacgaggtg cgtgacaacc tgctccgcca cacccggcaa gccggcgact
     4381 gaggcctgcc cgccgggttc aggggcgcgt ggcgtgcgct ccggcgcgct cgtccccctc
     4441 ggtccagccg tactggccga tgagcggcac gaacgcgacg ctgcccagcg tctgctgctc
     4501 gtagtcgtcc tgcgcgtgtc gcaccacctt gagcaggcgc tgcgtgccgt ggccgccgat
     4561 gtccaacggc atcagcagcc ggccgccgac gcgcagctgc gccttcagcg gcgccggcac
     4621 ctcggggccg gccgcggcga ccaggatcgc gtcgaacggc gcggcctcgg gccagccgag
     4681 cgtgccgtcg ccggtgcgca ggtggatgtt ggcgtaggcg agcgcgtcca gccgctcgcg
     4741 ggcggtgcgc gtcagcagct cgtggcgctc gatcgcgtgc accgtcgcgg ccacctgcgc
     4801 gagcacggcc gaggcgtagc ccgaaccggc gcccacctcc agcacgtggt cgccgggcac
     4861 gacctcggcg gcctcgatca tccaggccac catgaacggc tgggagatcg tctgttcagc
     4921 cgcgatcggc agcggcgaat cgtcgtacgc gaacgcggcc atgcgctcgt cgacgaagcg
     4981 ctcgcgcggc accgtgcgca tggcgcgcag cacatgctcg tcgcggatgc cgcggcccat
     5041 gagctggcgc tcgaccatgc ggtcgcgctc ggattggaag gaccggttca tggcgcctcc
     5101 tttcggccag ggccgtgagc cgggcgagca aggtgcatgc cccggccacc gtgaaattgt
     5161 gcgccgccgc ggcgtgggcg ggcgcgggtc cagatctccc cacacggcgc ctgcctcaat
     5221 ggcgctgcat cgccgtcgat aaggcgttga tggccgtgaa cctgtatggg acacgtgcca
     5281 ccccacgatg aagaccctgc ttcgccacct gcgcatcgga caccgcctcg tgctgtgctt
     5341 ctcgctgatc ctgctgctca tgatcggcgg cgcgtggctg gccgtgtcga gctcgcgcgc
     5401 ggcgcgcgag agcctcgtgc acctggtggc ggtctccaac gcgcgccagg ccgacatccg
     5461 cgcgatgcgc tcgctgctcg agcgccagga ccgcgtgtcg cagcgcctgg gcctgaccaa
     5521 ctcgatcgag gacgcacgca acgacatgca gacgatcgtc gaggacatcg cggcctaccg
     5581 cgtcgtcgag cggcgctacg cgagccatgt cgactcggtg ctcgagcgct cgatggtcga
     5641 gcagatcgag cactacgacc gcacgctcgc ggccgccttc gcgcaggcgc aggacagcgt
     5701 cgcggcgtac aaccccggca tggccgcgcg cacgctcaac cgcgacgtcg cgccgatcca
     5761 tgccgcgtgg ctgcacctgc tggacgacct cgccgagctg cagaaccagc gcgtggccgc
     5821 cgagatccag gcgctcagcg cgcacgccaa ccgcaacgac ctcgttatcg gcgccgtggc
     5881 gctgcttgcg tcgctgctgg ccgcgctcgc gggctggcgc ctgacgctga gcatcacgcg
     5941 cccgctcagg caggccgtgc tgttcgcctc tgcggtgggc agcggccagc tggacgcccc
     6001 gctgccgcgc agctccgacg acgaatgcgg catgctgctg ctggcgttga aggcgatggc
     6061 gcggcaactg caggaggcca acgcgcgcat gcagcgcctg ggcctgacca actcgatcga
     6121 ggacgcacgc aacgaca
//
LOCUS       DATCRO010000795         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_492703, whole genome shotgun sequence.
ACCESSION   DATCRO010000795 DATCRO010000000
VERSION     DATCRO010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492703"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VIN75_21680"
     CDS             complement(<1..1785)
                     /locus_tag="VIN75_21680"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY8976840.1"
                     /translation="MTAAGVSRERSQRLSAPSPRARLLAHGLACAAALACAASAHAAT
                     RELVVDAPSPFKALLEKNLDIERAARLAESDSPDDIEWARLIAAAPAQGRALAQTEGY
                     FRAEVTVTPDPADPHRILVKLVPGAPATVGRFTLEFDGELARAAESGDQKALDLEATL
                     RSEWQMQPGQVFRNGNWDGAKAQTLSALRAEGYASALWTATAAQVDPATNKARLFLVV
                     DTGPRFLAGDLVVEGIERQAESNVRQLAGFGLGAPLTQKRLLDYQDRLQKTGLFDQVA
                     VIYDPDPAQAAHATVTVRVHEQSLQQATVALGWNSLTQERVTLEHVDRRIFGLPATLS
                     NKVQWGKDVQAWDASLATHPAESFHSWITGISVSSIKSTSDLVRAGSVRFGRTQNSNA
                     LDRMTYVQLERSMQCTPHRSTQAPTVPAGLLSERKYDCVDARALSLNQANVWRNVDSV
                     ILPTDGYSLSGQVGVGYAGGPASFNDDQATYGPYARMYGRITRYWPLADSFFLQGRLE
                     LGQIVTRPHVAMPDAEQWRAGGVDSVRGYDWRSLAPHDSRGNIVGGNSLVTASLEIAH
                     PFTQSLPSVWWATFVDVGSAATRFSALKW"
     gene            complement(1782..2948)
                     /locus_tag="VIN75_21685"
     CDS             complement(1782..2948)
                     /locus_tag="VIN75_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976841.1"
                     /translation="MLIPFFYTLREARLPVSVKEYLTLLEALKAGVIGPSIDDFYFLS
                     RATLVKDEAHFDRFDKAFGAFFNGVAKLTESKEIPEDWLRQLIERELTPEQKAAIAAM
                     GWDELMETLRKRLEEQKGRHEGGNKWIGTGGTSPFGHGGYNPQGVRIGGAGKNRSAVK
                     VWEQRAYRDYDDSLELGTRNIKVALRRLRRFAREGADLELALDDTIHATAAAAGMLDI
                     KMQPERHNKVKVLLLMDVGGTMDEHIHRVEELFSAAKSEFKHLEFYYFHNCVYDYVWK
                     NNRRRFSEKHATWDVIRKYNKDYRLIFIGDATMSPYEILQAGGSVEYNNAEPGAEWIQ
                     RLTHAFPKHCWINPEPQGVWQYRQSISLIEQLVGHRMFPLTLQGLEGAMRLLTK"
     gene            3167..4723
                     /locus_tag="VIN75_21690"
     CDS             3167..4723
                     /locus_tag="VIN75_21690"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8976842.1"
                     /translation="MNFFSSLRIGRRLTVAFAVVVALTAVSALFSLWRLVEVRSAAVE
                     LTSVQAERTSLAFRWRQNVAINGSRAQMLAMITDMTLAEPLKAKVKTTSAETSVIQKR
                     FGELETTAQGQALQARLGEIRGRYLAGREALEKASADTSSSIASAIAKGQANNAFGAV
                     VDEYLAAGDALVSYEQKRAESEGADIVAAVNATSLVCVVCALVSVVFSIGLGVLLTRS
                     IVGPVKAAQKAADRIASGDLTAECRAVGSDESAQLVRSLARMQDSLIRIVREIRSSTE
                     QIQVASTEVASGNQDLSARTEQTAGSLQQAASSMDELSSTMRHSAEAANQAHGLANSA
                     SEIATRGGQVVSRVVSTMDEINHSSKKIADIIGVIDGIAFQTNILALNAAVEAARAGE
                     QGRGFAVVAGEVRSLAQRSAEAAKEIKSLIGNSVDKVETGAKLVQDAGSTMDEIVSAV
                     RRVSEVLGEITTATNEQAGGIQSVNEAVNSVDQMTQQNAALVEQSAAAAESMRDQAGK
                     LARTVEQFRT"
     gene            4830..>5011
                     /locus_tag="VIN75_21695"
     CDS             4830..>5011
                     /locus_tag="VIN75_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976843.1"
                     /translation="MRSLACVVTLGLVVLAGCADGPGKPPRDAVARREVCDPTAFGAR
                     ADGITKDTGALQAAIDA"
BASE COUNT          740 a         1719 c         1808 g          744 t
ORIGIN      
        1 ccacttgagg gcgctgaagc gcgtggccgc gctgccgacg tcgacgaagg tcgcccacca
       61 gaccgatggc agcgactgcg tgaacgggtg cgcgatctcg aggctggccg tcaccagcga
      121 gttgccgccg acgatgttcc cgcgcgaatc gtgcggggcc agggagcgcc agtcgtagcc
      181 gcgcaccgag tccacgccgc cggcgcgcca ttgctcggcg tcgggcatgg ccacgtgcgg
      241 ccgcgtgacg atctgtccca gctccagccg gccctgcagg aagaacgaat cggccagcgg
      301 ccagtagcgc gtgatgcggc catacatccg cgcatagggg ccgtaggtgg cctggtcgtc
      361 gttgaacgag gccgggccgc cggcgtagcc gacgcccacc tggccggaca gcgagtagcc
      421 gtcggtcggc aggatgacgc tgtcgacgtt gcgccagacg ttggcctggt tcagcgacag
      481 ggcgcgggcg tcgacgcagt cgtacttgcg ctcgctcagc aggcccgccg gcaccgtggg
      541 cgcctgggtc gaccggtgcg gcgtgcactg catcgagcgc tcgagctgca cgtaggtcat
      601 gcggtcgagc gcgttgctgt tctgcgtgcg gccgaagcgc acggagccgg cgcgcacgag
      661 gtcgctggtc gacttgatgc tgctgacgct gatgccggtg atccagctgt ggaagctctc
      721 ggccggatgc gtggccagcg aggcgtccca ggcctggacg tccttgcccc actgcacctt
      781 gttgctgagc gtggcgggca ggccgaagat cctgcggtcg acgtgctcca gcgtgacgcg
      841 ctcctgggtc agcgagttcc agccgagcgc cacggtggcc tgctgcagcg actgctcgtg
      901 cacgcgcacc gtcaccgtcg cgtgcgcggc ctgcgcgggg tcgggatcgt agatgacggc
      961 gacctggtcg aacaggccgg tcttctgcag ccggtcctgg tagtcgagca ggcgcttctg
     1021 ggtcagcggc gcacccaggc cgaagccggc gagctggcgc acgttggact cggcctggcg
     1081 ctcgatgcct tcgacgacga ggtcgcccgc caggaagcgc gggccggtgt cgaccaccag
     1141 gaacaggcgc gccttgttgg tggccggatc gacctgcgcg gccgtcgccg tccacagcgc
     1201 cgacgcatag ccctctgcgc gcagcgcgga cagcgtctgc gccttggcgc cgtcccagtt
     1261 gccgttgcga aagacctggc ccggctgcat ctgccattcg gagcgcagcg tggcctccag
     1321 gtcgagcgcc ttctggtcgc cgctctcggc ggcgcgcgcg agctcgccgt cgaactccag
     1381 cgtgaagcgg ccgaccgtgg ccggcgcgcc gggcacgagc ttgacgagga tgcgatgcgg
     1441 gtcggccgga tcgggcgtca ccgtcacctc ggcacggaag tagccctccg tctgcgccag
     1501 cgcgcggccc tgcgcgggcg cggccgcgat caggcgcgcc cactcgatgt cgtcggggct
     1561 gtcggactcg gccaggcgcg cggcgcgctc gatgtccagg ttcttctcga gcagcgcctt
     1621 gaacggcgac ggggcgtcca cgacgagctc gcgcgtggcg gcgtgcgcgg acgcggcgca
     1681 ggcgagcgcg gccgcgcagg cgagcccgtg cgcgagcagg cgcgcgcgtg ggctcggggc
     1741 cgacagtcgc tgggagcgtt cgcgcgagac gcccgcggcc gtcatttcgt caggagacgc
     1801 atcgcaccct ccaacccttg cagggtcagc gggaacatgc ggtggccgac gagctgctcg
     1861 atcagcgaga tgctttgccg gtactgccag acgccctgcg gctcgggatt gatccagcag
     1921 tgcttgggga acgcgtgcgt gaggcgctgg atccactccg cgccgggctc ggcgttgttg
     1981 tattccacgc tgccgccggc ctgcaggatc tcgtacgggc tcatcgtggc gtcgccgatg
     2041 aagatgagcc ggtagtcctt gttgtacttg cggatgacgt cccaggtggc gtgcttctcg
     2101 gagaagcggc ggcggttgtt cttccacacg tagtcgtaga cgcagttgtg gaagtagtag
     2161 aactccaggt gcttgaactc gctcttggcg gccgagaaca gctcctcgac gcggtggatg
     2221 tgctcgtcca tcgtgccgcc cacgtccatc agcagcagca ccttgacctt gttgtggcgc
     2281 tcgggctgca tcttgatgtc gagcatcccg gcggcggccg ccgtggcgtg gatggtgtcg
     2341 tccagggcca gctcgaggtc ggcgccctcg cgcgcgaagc ggcgcaggcg gcgcagcgcc
     2401 accttgatgt tgcgcgtgcc cagctcgagc gagtcgtcgt agtcgcggta ggcgcgctgc
     2461 tcccagacct tgaccgcgct gcggttcttg ccggcgccgc cgatgcgcac gccctggggg
     2521 ttgtagccgc cgtggccgaa cggcgacgtg ccgccggtgc cgatccactt gttgccgccc
     2581 tcgtggcggc ccttctgttc ctcgagccgc ttgcgcagcg tctccatcag ctcgtcccag
     2641 cccatggcgg cgatggcggc cttctgctcg ggcgtgagct cgcgctcgat cagctggcgc
     2701 agccagtcct cggggatctc cttggactcg gtgagcttgg cgaccccgtt gaagaaagcg
     2761 ccgaacgcct tgtcgaagcg gtcgaaatgc gcctcgtcct tgaccagcgt ggcacgcgac
     2821 aggaaataga agtcgtcgat cgacggcccg atgacccccg ccttgagcgc ttcgagcagc
     2881 gtcaggtact ccttgaccga gaccggcagc cgggcctcgc gcagggtgta gaagaagggg
     2941 atcagcatgg ggcgggcgga cgtcggcttg agcgcgaagg gtatcaccgt tgcatcgccg
     3001 agtcggacgc agggctcaag ctcggccgca aactgccgat gacgcagaaa tgcacccttg
     3061 tggggcggct gatcgcgcca tcgcaccggc gcggcgcccc cagaatttcc agtgggaccg
     3121 gacgcccggg ggcgtgcgtc cggcccgatt cccggagaca gagaacatga acttcttcag
     3181 cagcttgcgc atcggccgcc ggctcaccgt cgcgttcgcc gtcgtggtgg cgctgaccgc
     3241 cgtgtcggcg ctgttcagcc tgtggcgcct cgtggaggtc cggtcggccg cggtggagct
     3301 gaccagcgtg caggccgagc gcacgtcgct ggccttccgc tggcgccaga acgtggccat
     3361 caacggcagc cgcgcgcaga tgctggcgat gatcacggac atgacgctgg ccgagccgct
     3421 caaggccaag gtcaagacca cgagcgccga gacgtcggtg atccagaagc gcttcggcga
     3481 gctggagacc acggcccagg gccaggcgct gcaggccagg ctgggcgaga tccgcgggcg
     3541 ctacctcgcc gggcgcgagg cgctggagaa ggccagcgcc gacaccagca gctcgatcgc
     3601 gtcggccatc gccaagggcc aggccaacaa cgcgttcggc gccgtcgtcg acgaatacct
     3661 ggcggccggc gacgcgctcg tcagctacga gcagaagcgc gccgagagcg aaggcgccga
     3721 catcgtggcc gccgtcaacg ccaccagcct ggtgtgcgtc gtgtgcgcgc tggtcagcgt
     3781 cgtgttctcg atcggcctgg gcgtgctgct cacgcgcagc atcgtgggtc cggtcaaggc
     3841 cgcccagaag gccgcggacc gcatcgcctc gggcgacctg accgccgagt gccgcgccgt
     3901 cggcagcgac gagtccgcgc agctggtgcg gtcgctggcg cgcatgcagg actcgctgat
     3961 ccgcatcgtg cgcgagatcc gcagctccac cgagcagatc caggtcgcga gcaccgaggt
     4021 ggccagcggc aaccaggacc tgagcgcgcg caccgagcag acggccggca gcctgcagca
     4081 ggccgccagc tcgatggacg agctcagcag caccatgcgt cacagcgccg aggccgccaa
     4141 ccaggcgcac ggcctcgcga actcggcctc cgagatcgcc acgcgcggcg gccaggtcgt
     4201 ctcgcgcgtc gtctccacga tggacgagat caaccacagc tccaagaaga tcgccgacat
     4261 catcggcgtc atcgacggca tcgcgttcca gaccaacatt ctggcgctga acgccgcggt
     4321 cgaggccgcg cgcgccggcg agcagggccg cggcttcgcg gtggtggccg gcgaggtgcg
     4381 cagcctcgcg cagcgctcgg ccgaggcggc caaggagatc aagagcctga tcggcaactc
     4441 ggtcgacaag gtcgagaccg gcgcgaagct ggtgcaggac gcgggctcga cgatggacga
     4501 gatcgtcagc gccgtgcgcc gcgtcagcga ggtgctgggc gagatcacca ccgccaccaa
     4561 cgagcaggcc ggcggcatcc agtcggtcaa cgaggcggtc aactccgtcg accagatgac
     4621 gcagcagaac gcggcactcg tcgagcagtc ggcggcggcg gccgaatcga tgcgcgacca
     4681 ggccggcaag ctggcgcgca cggtggagca attccggacc tgaacccgct cgcctcgggc
     4741 aagggcgcac gacctgcggg tctgcgccct tcgtcgttcc ggcggctcgc gatagagtgc
     4801 gtgccacacg tcgaacccgg agtccctcca tgcgctcgtt ggcctgcgtc gtcacgctcg
     4861 gtcttgtcgt cctcgcaggc tgcgccgacg ggcccggcaa gccgccccgg gacgccgtcg
     4921 cccgccgcga ggtctgcgat ccgaccgcct tcggcgcccg cgccgacggc atcaccaagg
     4981 acaccggcgc gctgcaggcc gccatcgacg c
//
LOCUS       DATCRO010000796         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_796845, whole genome shotgun sequence.
ACCESSION   DATCRO010000796 DATCRO010000000
VERSION     DATCRO010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796845"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VIN75_21700"
     CDS             <1..327
                     /locus_tag="VIN75_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY8976844.1"
                     /translation="APLSLVFDAAGAGAIDRPVFLYYGDADHVLRPVENAARLAPLVP
                     TLVGVKPVAGADHWSFIPACSPELAQANPMICADAPGISRAALHARIDADALSFFRAT
                     LHVAPE"
     gene            465..1136
                     /locus_tag="VIN75_21705"
     CDS             465..1136
                     /locus_tag="VIN75_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY8976845.1"
                     /translation="MPVPPLAPAAATLPTSDETPSILPEAVDATGPHSLLDDESPRQP
                     ASPGEIFRVFTRLALKGFGGVLPIVQHELVERERWLTREQFLELLAVAQVLPGPNVVN
                     LSLMFGDRAFGWRGGLAALGGMLLAPLGVVLALTVAYAHYAQHPVVAGVLRGMGAVAA
                     GLIMSTGIKLLPALRRNVLGPTLAFGFVAVMFACIALLRVPLPWMLGGLGAVAFVLAW
                     HRLKP"
     gene            1133..1681
                     /locus_tag="VIN75_21710"
     CDS             1133..1681
                     /locus_tag="VIN75_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY8976846.1"
                     /translation="MTPAVVSSPSGADWGGVLAQFLLLSLLSIGGAISTVPEMHRYLV
                     DQHHWLSNDQFSAMVAIAQAAPGPNLLFVAVLGWGIGGALGMLVTMLGILLPSTTLTL
                     AAARWGEARRHTRGVQAFVTGLAPLTVGLVLSSGFLLGKPAYGDPWLIAAMIVTVVVC
                     VRWKVSPLWLIAAGGLVGGLVT"
     gene            complement(1674..>3402)
                     /locus_tag="VIN75_21715"
     CDS             complement(1674..>3402)
                     /locus_tag="VIN75_21715"
                     /inference="COORDINATES: protein motif:HMM:NF021430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HEY8976847.1"
                     /translation="LIQQRGTRLVRIDGAGFEQAAVASWRPEGGRLVVAGRFTGAKAM
                     LDLVLESGDGRTLHKTIELAAPKGGDDAPTGFVAQRWARLRVDELMAQPQLHRTEITA
                     LGKRYAIVTPETSLIVLETLEDYQRYELEPPPGPLREAYDAAARNVAAGKAAQSARHL
                     ESLVQRWREVQAWWDKDFPKDEPAPQKIAAADAPFAARAVQAQPRNAPVMAASVAPPV
                     STDAAPMAAPAPAPVLDLAEAPRERAAVHVAEARAVPDSRDGASRLKKTEGNTSAPST
                     IHIAVPAWSPDAADMRRLADAAPDARYAVYLDQRLEASGSPAFYLDAAQVFASHGQRA
                     LALRVLSNLAELHLEDRGLLRVLAYRLQETGETAQAIRVLRRVAELAPDEPQSWRDLG
                     LAQAAAGAWQPAVDALWTVASGTWDGRFGDIDVIALGELDAIAAVHPVDMSPVDARLR
                     RNLPLDLRVALAWDTDNTDIDLWVKDPHGEWVSYQQPLSRQGGRITHDVTNGYGPEVF
                     SLKKAIPGVYEVRARYFGSHRQALGNATSVMMRLTTGFGTKGEKHRDVILRLEEARDE
                     VLVGSFEVR"
BASE COUNT          396 a         1273 c         1211 g          522 t
ORIGIN      
        1 gcgcctctca gcctggtctt cgatgcggcc ggcgccggcg cgatcgaccg ccccgtcttc
       61 ctctattacg gcgacgccga tcatgtcctg cgtccggtcg agaacgccgc gcgcctcgcg
      121 ccgctggtgc ccacgctggt cggcgtcaag ccggtcgccg gagccgacca ctggtcgttc
      181 atccctgcgt gcagcccgga gctcgcgcag gccaacccca tgatctgcgc cgatgcgcca
      241 ggcatctcgc gcgcagcgct gcacgcccgg atcgatgccg acgcactgtc gttcttccgc
      301 gcgacccttc acgtggcgcc ggagtgagcc ggcccggcag cggcacgggc gatgccggcg
      361 ggcgtcggcc aatcccgaca actcaagtct tatataagac ataggacttt cctcgaccgc
      421 gacgacggag tacgctggcg gcaaccgctc acgaagccat gtcgatgccc gttccgcccc
      481 tcgctcccgc tgccgccacc ctgccgacgt cggacgagac gcccagcatc ctgcccgagg
      541 cggtcgatgc gaccggcccg cattccctgc tcgacgacga gtcgccgcgg cagccggcgt
      601 cgccgggcga gatcttccgc gtcttcacgc gcctggcgct caagggcttc ggcggggtgc
      661 tgccgatcgt gcagcacgag ctggtggagc gcgaacgctg gctgacgcgc gagcagttcc
      721 tcgagctgct ggcggtcgcc caggtgctgc ccggcccgaa cgtcgtcaac ctgtcgctga
      781 tgttcggtga tcgcgcgttc ggctggcgcg ggggcctggc ggcgctgggc ggcatgctgc
      841 tcgcgccatt gggcgtcgtg ctggcgctga ccgtggccta tgcgcactac gcgcagcatc
      901 cggtggtggc cggcgtgctg cgcggcatgg gcgccgtcgc ggcgggcctg atcatgtcca
      961 ccggcatcaa gctgctgccc gccctgcgcc gcaacgtcct ggggcccacg ctggccttcg
     1021 gtttcgtggc cgtcatgttc gcgtgcatcg cgctgctgcg cgtgccgctg ccgtggatgc
     1081 tgggcgggct gggcgcggtc gccttcgtgc tggcctggca ccggttgaag ccatgacgcc
     1141 ggccgtcgtg tcctcgccgt ccggcgccga ctggggcggc gtgctcgcgc agttcctgct
     1201 gctgtcgctg ctgtccatcg gcggcgcgat cagcaccgtg ccggagatgc accgctacct
     1261 cgtcgaccag caccactggc tcagcaacga ccagttcagc gcgatggtgg cgatcgcgca
     1321 ggccgcgccg ggccccaacc tgctgttcgt cgcggtgctg ggctggggca tcggcggcgc
     1381 gctgggcatg ctggtcacga tgctgggcat cctgctgccg tccaccacgc tcacgctggc
     1441 cgccgcgcgc tggggcgagg cgcgccgcca cacgcgcggc gtgcaggcct tcgtcacggg
     1501 cctggcgccg ctgaccgtcg gcctggtgct gtcctcgggc ttcctgctgg gcaagccggc
     1561 ctacggcgat ccctggctga tcgccgcgat gatcgtgacc gtcgtcgtct gcgtgcgctg
     1621 gaaggtcagt ccgctgtggc tcatcgccgc cggcgggctg gtgggcggcc tcgtcacctg
     1681 acctcgaagc tgccgaccag cacctcgtcc cgggcctcct ccaggcgcag gatcacgtcg
     1741 cggtgcttct cgcccttggt gccgaagccg gtggtcagcc gcatcatcac gctggtggcg
     1801 ttgccgagcg cctggcgatg cgagccgaag tacctggcgc gcacctcgta gacgccgggg
     1861 atcgccttct tcagcgagaa cacctcgggc ccgtagccgt tggtgacgtc gtgcgtgatg
     1921 cgcccgccct ggcggctcag cggctgctgg tagctcaccc actcaccgtg gggatccttc
     1981 acccacaggt cgatgtcggt gttgtcggtg tcccaggcga gcgcgacgcg caggtccagc
     2041 ggcaggttgc ggcgcaggcg agcgtcgacg ggggacatgt cgaccgggtg caccgccgcg
     2101 atcgcgtcga gctcgccgag cgcgatgacg tcgatgtcgc cgaagcggcc gtcccaggtg
     2161 cccgacgcca ccgtccacag cgcgtcgacc gcgggctgcc aggcgccggc cgcggcctgc
     2221 gcgaggccca ggtcgcgcca ggactgcggc tcgtcgggcg cgagctcggc cacgcggcgc
     2281 agcacgcgga tcgcctgcgc cgtctcgccc gtctcctgca gccggtaggc cagcacgcgc
     2341 agcaggccgc gatcctccag gtgcagctcc gccaggttcg acagcacgcg cagcgccagc
     2401 gcgcgctggc cgtgcgacgc gaacacctgc gcggcgtcga ggtagaacgc cgggctgccc
     2461 gaggcctcga gacgctggtc gaggtagacc gcgtagcgcg cgtccggcgc cgcgtccgcc
     2521 agccggcgca tgtccgcggc gtcgggcgac caggccggga cggcgatgtg gatcgtcgac
     2581 ggcgccgatg tattgccctc ggtcttcttc aagcgggagg ccccgtcgcg gctgtccggc
     2641 acggcgcggg cctcggccac gtggacggcg gcgcgctcgc gcggcgcctc ggccaggtcc
     2701 agcacaggcg ccggcgccgg cgcggccatg ggcgcggcgt cggtcgacac gggcggcgcc
     2761 acggacgccg ccatgaccgg cgcattcctc ggctgggcct gcacggcccg cgccgcgaag
     2821 ggcgcatcgg ccgccgcgat cttctggggc gccggctcgt ccttcgggaa gtccttgtcc
     2881 caccaggcct gcacctcgcg ccagcgctgc accagcgact ccaggtgccg ggcggactgc
     2941 gccgccttcc ccgccgcgac gttgcgcgcg gcggcgtcgt aggcttcgcg cagcggcccc
     3001 ggcggcggct ccagctcgta gcgctggtag tcctccagcg tctcgagcac gatcagcgag
     3061 gtctcgggcg tcacgatggc gtagcgcttg ccaagcgccg tgatctccgt gcgatgcagc
     3121 tgcggctgcg ccatgagctc gtcgacgcgc agccgcgccc agcgttgcgc gacaaagccg
     3181 gtcggcgcat cgtcgccgcc cttgggcgcg gccaactcga tcgtcttgtg cagcgtcctg
     3241 ccatcgccgg actccagcac caggtccagc atcgccttgg cgccggtgaa gcgcccggcc
     3301 accaccagcc gtccgccctc gggccgccac gacgcgacgg cggcctgctc gaagcccgcg
     3361 ccgtcgatgc gcaccagccg cgtgccgcgc tgctggatca gc
//
LOCUS       DATCRO010000797         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_98869, whole genome shotgun sequence.
ACCESSION   DATCRO010000797 DATCRO010000000
VERSION     DATCRO010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98869"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VIN75_21720"
     CDS             complement(<1..1261)
                     /locus_tag="VIN75_21720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY8976848.1"
                     /translation="MKVEASPTSELTLRGVVLGFLITLLFTAANVYFGLKAGLTFATS
                     IPAAVISMALLRYARGVTIQENNIVQTVASAAGAISSIIFVLPGLVMVGWWTGFPYVT
                     CVIICALGGVLGVTYSIPLRRALVTTSDLPYPEGVACAEVLKVGAGGDGGAAENRAGL
                     LAIVWGGIVSALFQVVIAMQLFAADVVGFFRLSRSSNAATGVDFGLSLALLAVGHLVG
                     LWVGVAMLVGVLIAWAGAVPHLTGLLPFDTAADVAAMATKVWAKQVRFIGAGAIAVAA
                     VWTLVKLVKPVGTGLASAMAASRVRKAGQGSTLPRTEQDLPIGWVAGVTLACLVPVGW
                     LLADMSSGAGLGSATVILVAGGIAFVALMSFFVAAVCGYMAGLIGSSNSPLSGVGIIV
                     VVTAALLVALAAKPLLPAGASQALVAFT"
     gene            complement(1377..1685)
                     /locus_tag="VIN75_21725"
     CDS             complement(1377..1685)
                     /locus_tag="VIN75_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8976849.1"
                     /translation="MTNPCLSCGACCAAFRVSFHWVEAPAGLDARLTERIGRQHLCMA
                     GTNAAAPRCIALEGVVGGPTRCTAYDARPPACREVQPGDGKCTRARERHGLAPVPAAA
                     "
     gene            1955..>2985
                     /locus_tag="VIN75_21730"
     CDS             1955..>2985
                     /locus_tag="VIN75_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976850.1"
                     /translation="MIRHLHPLPRSLRRPVIALSIAAAFGLSLTGPARAQVSVDVGVS
                     AQEPGWLYDLGAVVSVFVDPPIQQPEPVAIAWAPPPMLVEDVPPQPDPYAVWTGGYWT
                     WQGDWVWCAGRWMDPPRQEYVWVQPYYEHRADRVVFIPGYWSAPDVEFIAPPEDREIA
                     WGYVNEGVVIGPPPEGPVGIFVPPPPGSRPGIIVPALIGTPPAVVVSAPPVMSVGMRI
                     SGTFAYDGRHTISDNHPVSYRPAALRNIQNVTVQAPAGATASGHPFQAQVPHRAALAA
                     AMHPQVAARAPVPASRTAVPAYVPGRPVAALPAPQKVQGLPAVPTHAAANLPQAQRAQ
                     MAHAPLTPA"
BASE COUNT          440 a         1137 c         1051 g          357 t
ORIGIN      
        1 gcgtgaacgc gaccagcgcc tgcgaggcgc ccgcgggcag cagcggcttg gcggcgagcg
       61 cgaccagcag cgccgccgtc acgacgacga tgatgcccac cccggagagc gggctgttgc
      121 tcgagccgat caggccggcc atgtagccgc agacggccgc cacgaagaag ctcatcagcg
      181 cgacgaacgc gatgccgccg gccaccagga tgacggtggc cgagcccagg cccgcgccgc
      241 tgctcatgtc cgccagcagc cagcccacgg gcaccaggca ggccagcgtg acgccggcga
      301 cccagccgat cggcaggtcc tgctcggtgc gcggcagcgt cgagccctgg ccggccttgc
      361 gcacgcgcga agcggccatg gccgaggcca ggcccgtgcc gacgggcttg accagcttga
      421 cgagggtcca gacggcggcc accgcgatgg cgccggcacc gatgaagcgc acctgcttgg
      481 cccagacctt ggtggccatg gcggcgacgt ccgcggcggt gtcgaacggc agcagcccgg
      541 tcaggtgcgg cacggcgccg gcccaggcga tcagcacgcc caccagcatg gccacgccca
      601 cccacaggcc caccaggtgg cccacggcca gcagcgccag cgacaggccg aagtcgacgc
      661 cggtggcggc gttgctgctg cggctcaggc ggaagaagcc cacgacgtcg gcggcgaaca
      721 gctgcatcgc gatcaccacc tggaacagcg cggagacgat gccgccccag acgatcgcca
      781 gcaggccggc acggttctcc gccgccccgc catcaccgcc cgcgcccacc ttgagcacct
      841 cggcgcaggc cacgccttcg ggatacggca ggtcggacgt ggtcacgagc gcgcgccgca
      901 gcgggatcga gtaggtcacg cccagcacgc cgccgagcgc gcagatgatg acgcaggtga
      961 cgtacgggaa ccccgtccac cacccgacca tcacgaggcc gggcaggacg aagatgatcg
     1021 acgagatcgc gccggccgcc gaggccaccg tctgcacgat gttgttctcc tggatcgtca
     1081 cgccgcgcgc gtagcgcagc aaggccatgg agatgaccgc cgccgggatc gacgtggcga
     1141 acgtcaggcc cgccttcagg ccgaaataga cgttggcggc cgtgaacagc agcgtgatca
     1201 ggaaaccgag cacgacgccg cgcagggtca gttcgctggt gggtgaagct tcgactttca
     1261 aggaatctcc tcgggatcaa gccgcgcatc atccctcaag tgcgcggcgg ccggtatggt
     1321 gactacgcgc agcgcccggc gcgccgcacg gcctgccggc attgtccgcg aggccgtcag
     1381 gcggccgcgg gcacgggcgc caggccgtgg cgctcgcgcg cccgggtgca ttttccgtcg
     1441 cccggctgga cctcgcggca ggccggcggc cgcgcgtcgt aggcggtgca gcgggtgggc
     1501 ccgccgacca ccccctcgag cgcgatgcag cgcggcgcgg ccgcattggt gccggccatg
     1561 cacaggtgct gccggccgat gcgctccgtc aggcgcgcat cgagtccggc gggcgcctcg
     1621 acccagtgga acgagacgcg gaaggcggcg cagcaggcgc cgcaggacag gcaggggttg
     1681 gtcatcggca ggcggatcga accgccattc tggccgcttc gcggcgaagt tcgtgagcag
     1741 ccactgcgcg cccgtggccc ggtcgccact gccgagaagg gacgacggca gcttgttccg
     1801 gcggacgccc ccgctcggcc atgcgatgcg gccttgtccg acatcgcccg cgccgcccgg
     1861 cgcgttggac cggcaagcgg cacaacgaat gccgacgttc atgcatcgct ggcgccgccc
     1921 ggcgctcccc cttccgacaa ctccggagac aaccatgatc cgacacctcc atcccctccc
     1981 ccgcagcttg cgccgacctg tcatcgcgct gtcgatcgcc gcggccttcg gcctttccct
     2041 gaccggcccc gcgcgggcgc aggtgtccgt cgacgtcggc gtcagcgccc aggaacccgg
     2101 ctggctgtac gacctcggcg ccgtcgtcag cgtgttcgtc gatcccccga tccagcagcc
     2161 ggagccggtc gcgatcgcgt gggcgccgcc gccgatgctg gtcgaggacg tgccgcccca
     2221 gcccgacccg tatgccgtct ggaccggcgg ctattggacg tggcagggcg attgggtctg
     2281 gtgcgcgggc cgttggatgg atccgccgcg ccaggaatac gtgtgggtgc agccgtacta
     2341 cgagcaccgc gccgatcgcg tcgtcttcat cccgggctac tggtcggcgc ccgacgtcga
     2401 gttcatcgcc ccgccggagg atcgcgagat cgcctggggc tacgtcaacg aaggcgtcgt
     2461 gatcgggccg ccgcccgagg gcccggtcgg catcttcgtg ccgccgccgc cggggtcgcg
     2521 cccgggcatc atcgtgccgg cgctgatcgg cacgccgccg gcggtggtgg tcagcgcgcc
     2581 gccggtgatg tcggtgggca tgcgcatctc cggcaccttc gcctacgacg gacgccacac
     2641 catcagcgac aaccacccgg tcagctaccg ccccgccgcc ttgcgcaaca tccagaacgt
     2701 gaccgtgcag gcgcccgccg gcgccaccgc gagcggccac ccgttccagg cgcaggtgcc
     2761 gcaccgcgcc gcgctggccg ccgccatgca tccgcaggtg gccgcgcgcg cgccggtgcc
     2821 cgcgtcgcgc accgccgtgc cggcctacgt gccggggcga ccggtcgccg cgctgcccgc
     2881 gccgcagaag gtgcaggggc tgcccgccgt gcccacccac gcggccgcca acctgccgca
     2941 ggcccagcgc gcccagatgg cccacgcccc gctgacgccc gccga
//
LOCUS       DATCRO010000798         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_729134, whole genome shotgun sequence.
ACCESSION   DATCRO010000798 DATCRO010000000
VERSION     DATCRO010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729134"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VIN75_21735"
     CDS             <1..1032
                     /locus_tag="VIN75_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8976851.1"
                     /translation="MLQTALRRVMAPAKSASTSHEPSSYLHYGEPAGDTRLRHALAQR
                     LADFGVPANPTQIVTANGATHALDICSRALLAPGDAVLVDEPGWSVEFARLTQLGMRL
                     LPVPRGVDGPDLAAMAALAQAHRPRLYVTCSVLHNPTGASLGLAAAHQVLRLAEQHDF
                     RILEDDTYAHLAPAHAPRLSALDGLRRTIYVSGFAKILAPGWRVGFMAAPPDLVERFV
                     DVKLLGTLTTPALLEQAVAVCLEQGWLRRHAERVITRLGAARARTVKLALAAGCRFVT
                     PPAGLFGWVDTGVDTERLATDMLDDGWLLAPGTVFHPGRQPSTLMRINFATTQDPRFW
                     QAFEQAREG"
     gene            complement(1055..1363)
                     /locus_tag="VIN75_21740"
     CDS             complement(1055..1363)
                     /locus_tag="VIN75_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976852.1"
                     /translation="MKTSLRQFALVSTLLATAALPLLSHAQDVRREDRVLHFLDSGHI
                     DANKDGMVSKKEFMDAMSKTWDMHMDDAKKADPMMHKDKMTLQQYLEFSKMFGLNVGS
                     "
     gene            complement(1498..2700)
                     /locus_tag="VIN75_21745"
     CDS             complement(1498..2700)
                     /locus_tag="VIN75_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976853.1"
                     /translation="MLMLLLACVLSLGGWVVVAPALRGTGRDVVEPPADLSSSEAAPT
                     RRAGAAAPAASAMPAVAGAARAFVPQAVDARAAETTDARCGEDQLPVYRPAERDADGA
                     IHVEAPVPDPDGIVRRLPGEIKSAGVGYTGAMSRLDAALRSSGDPFDRAMADWLDLDL
                     IEPPVRRQEALVRDAVATSDARVYGLAYAACNVPAVVPDAPPACAELSPDVWAQLDPG
                     NAKPWLYALQRADAAGDVAAGRVALDRLAGSTRLDVRFHAGAAAVARQRLGDADLAAQ
                     SMTVARALGMPQPPFQALTARCRDGAGGDPALAATCARIADVFYEHSDTYMGRAIGAS
                     LHKIATGDAGWLDRVHQEQRERRGPDAVAPGNAPCAGQRAFLALFVRLDEVGETHLAR
                     QAASAPRP"
     gene            complement(2835..>3741)
                     /locus_tag="VIN75_21750"
     CDS             complement(2835..>3741)
                     /locus_tag="VIN75_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976854.1"
                     /translation="RYDLATARRLGEILKKACVVAVDYIMKNAIPYLGGAIDLGTGLF
                     RTISEAGTRLAAWNDRRHIRLQAGHPEELANAIEHQMSLGVLGGVADMLKGASKVAVG
                     VFLPGLGSLVSAVMSGIEWLVRLVSRLGEYFGIQQFLQTARRHYGAEKARATRDAKGV
                     YEPNTAAGGIINDTTAFTKFFKDGCKASPLIPMLTLNSGLGGSLMTMLQLFEADGSQS
                     ARATRNVTGGETREFDRGDMYFTRLKRWSVEYMRKSGFKFLPADAKDVVMAGYLAHAT
                     GMGKERQSHVEAGTAMGRVGAFLTA"
BASE COUNT          518 a         1373 c         1308 g          542 t
ORIGIN      
        1 atgctgcaga ccgctctgcg gcgcgtgatg gcgccggcga agtcggccag caccagtcac
       61 gagccctcga gctacctgca ctacggcgag cccgccggcg acacgcgcct gcgccacgcg
      121 ctcgcgcagc gcctggccga cttcggcgtg ccggccaacc cgacgcagat cgtcaccgcc
      181 aacggcgcca cgcacgcgct ggacatctgc tcgcgcgcgc tgctcgcgcc cggcgacgcg
      241 gtgctggtcg acgagccggg ctggtcggtg gagttcgcac gcctgaccca gctaggcatg
      301 cgcctgctgc cggtgccgcg cggcgtcgac ggccccgacc tggccgcgat ggccgcgctc
      361 gcgcaggcgc accgtccgcg gctgtacgtc acgtgttcgg tgctgcacaa cccgaccggc
      421 gcgtcgctgg gcctggccgc tgcgcaccag gtgctgcgcc tggccgagca gcacgacttc
      481 cggatcctcg aggacgacac ctacgcgcac ctcgcgccgg cgcacgcgcc gcgcctgtcc
      541 gcgctcgacg gcttgcgacg cacgatctac gtgtcgggct tcgccaagat cctcgcgccg
      601 ggctggcgcg tgggcttcat ggcggcaccg cccgatctcg tcgaacgctt cgtcgacgtc
      661 aagctgctgg gcacgctcac gacacccgcg ctgctcgaac aggccgtggc cgtctgcctg
      721 gagcagggct ggctgcgccg ccacgccgag cgcgtgatca cgcgcctcgg cgcggcccgc
      781 gcgcgcaccg tcaagctggc actggccgcc ggctgccgtt tcgtgacgcc gcccgccggc
      841 ctgttcggct gggtggacac cggcgtcgac accgaacgcc tggccaccga catgctcgac
      901 gacggctggc tgctggcccc cggcacagtc ttccaccccg gccgccagcc gtcgacgctg
      961 atgcgcatca acttcgcgac gacgcaggat ccgcggttct ggcaggcgtt cgagcaggcg
     1021 cgcgagggct gagggcgcgc cgcgtcgcgc gcgttcagct gccgacgttc aggccgaaca
     1081 tcttcgagaa ttcgaggtat tgctgcagcg tcatcttgtc cttgtgcatc atgggatcgg
     1141 ctttcttggc gtcgtccatg tgcatgtccc aggtcttgga catcgcgtcc atgaattcct
     1201 tcttgctgac catgccgtcc ttgttggcgt cgatgtggcc gctgtccagg aagtgcagga
     1261 cgcggtcctc gcggcgcacg tcctgcgcat gcgagagcag cggcaaggcg gcggtcgcga
     1321 gcagggtcga gacgagggcg aattggcgca gggaagtctt cacgatggtt tccttcgaac
     1381 ggttggaggg catggccacg acggccgccg gcagcgcggt tcacgccgcg gagagcgcgg
     1441 gttgtgcgag ccagtgaccg gtagtcgcgc ggatcgagga aatccttacg cgcggcgtca
     1501 gggccgcggc gccgacgcgg cctggcgcgc cagatgcgtt tccccgactt cgtccagccg
     1561 gacgaaaagc gcgagaaagg cacgctggcc tgcgcacggt gcgttgccag gcgccacggc
     1621 atcggggccg cggcgctcgc gctgttcctg gtggacgcga tcgagccagc cggcgtcgcc
     1681 cgtcgcgatc ttgtgcagcg aagcgccgat ggcgcgaccc atgtacgtgt cggagtgctc
     1741 gtagaagacg tcggcgatgc gggcgcacgt cgcggcgagg gccggatcgc ctccggcccc
     1801 gtcgcgacaa cgggccgtca acgcctggaa gggcggctgc ggcatgccca gggcccgggc
     1861 gacggtcatc gactgcgccg ccaagtcggc atcgcccagg cgctggcgcg ccaccgcggc
     1921 ggcgccggca tggaagcgga cgtccagccg cgtcgacccg gccaggcggt cgagcgccac
     1981 gcgcccggcc gcgacatcgc ccgctgcgtc ggcgcgttgc aacgcgtaca gccagggctt
     2041 cgcatttccc ggatcgagct gcgcccacac gtcgggtgac agttcagcgc aggccggtgg
     2101 cgcgtcaggc accaccgcag gcacgttgca agccgcgtag gccagcccgt acacacgggc
     2161 gtcggaggtc gcgaccgcat cgcggaccag cgcttcctgg cgccgcacgg gcggctcgat
     2221 gagatcgaga tccagccaat cggccattgc gcggtcgaag gggtcgccgc tggaccgcaa
     2281 cgcggcgtcg agacggctca tcgcgccggt gtagccgacg ccggcggact tgatctcgcc
     2341 gggcagccgc cgcacgatgc catcgggatc cggcaccggc gcctcgacgt ggattgcgcc
     2401 gtccgcgtcg cgctcggccg gccggtagac cgggagctgg tcttcgccgc agcgggcgtc
     2461 ggtcgtctcg gctgcccgcg cgtcgaccgc ctgcggcacg aacgcgcggg ccgcacccgc
     2521 gacggccggc atcgccgacg ccgccggcgc cgcggcgccg gcgcgccggg taggcgccgc
     2581 ttccgaagac gacaggtccg cgggcggttc cacgacgtcg cggcccgttc cacgcaacgc
     2641 cggcgcgacc accacccagc cgcccagcga gagcacgcag gcgagcagca gcatcagcaa
     2701 caggacgatt cttcgacggg acacggcatc cgggcgatgg agacggcctg cccattctgg
     2761 tcacggtccg tcgaacccga cccgcgcatt catcgcgata aaccgacgat tcggcgctcg
     2821 cgggcgcccg ccgctcacgc cgtcaggaac gcccccacgc gacccatcgc cgtgccggcc
     2881 tcgacgtggc tctggcgctc cttgcccatg cccgtggcgt gcgccaggta gcctgccatc
     2941 accacgtcct tcgcatcggc cggcaggaac ttgaagccgc tcttgcgcat gtactccacc
     3001 gaccagcgct tgagccgcgt gaagtacatg tcgccgcggt cgaactcgcg ggtctcgccg
     3061 ccggtcacgt tgcgcgtggc gcgggccgac tggctgccgt cggcctcgaa cagctgcagc
     3121 atcgtcatga ggctgcctcc caggcccgag ttgagcgtga gcatcgggat cagcgggctc
     3181 gccttgcagc cgtccttgaa gaacttcgtg aaggccgtgg tgtcgttgat gatgccgccg
     3241 gccgcggtgt tgggctcgta gacgcccttg gcgtcccggg tcgcgcgcgc cttctcggcg
     3301 ccgtagtgcc ggcgcgccgt ctgcaggaac tgctggatgc cgaagtactc gcccagccgc
     3361 gagacgagcc gcaccagcca ttcgatgccc gacatcaccg ccgacaccag cgagcccagc
     3421 cccggcagga acacgcccac ggccaccttg ctggcgccct tgagcatgtc ggccacgccg
     3481 cccaggacgc ccagcgacat ctggtgctcg atcgcgttgg cgagctcctc gggatgcccg
     3541 gcctgcaggc ggatgtggcg gcggtcgttc cacgcggcca gccgcgtgcc ggcctcgctg
     3601 atggtgcgga acaggccggt gcccaggtcg atcgcgccgc ccaggtacgg gatcgcgttc
     3661 ttcatgatgt agtcgaccgc gacgacgcag gccttcttca ggatctcgcc cagccggcgc
     3721 gccgtcgcca ggtcgtagcg c
//
LOCUS       DATCRO010000799        10122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_357404, whole genome shotgun sequence.
ACCESSION   DATCRO010000799 DATCRO010000000
VERSION     DATCRO010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357404"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIN75_21755"
     CDS             <1..217
                     /locus_tag="VIN75_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-peptide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HEY8976855.1"
                     /translation="RSGDDYVELATALANEPDTLRGIKAHLDQNRRVLPLFDAERVAR
                     DMDALLMRMHARHLAGLAPAALAAEQA"
     gene            214..1278
                     /locus_tag="VIN75_21760"
     CDS             214..1278
                     /locus_tag="VIN75_21760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8976856.1"
                     /translation="MSSYKVALVMIARNEAARIVRALASATNCVDELVVLDTGSTDAT
                     VALARSAGARVHHFKWCDDFAAARNAALELADADWNVVVDADEWIAEAGIELLALRHT
                     APGFVGTLRVDSQFDTPIGTSVAPSWLPRVLPRGVRYEGHVHEQPEHRLPVRRLATVL
                     AHDGYRADALKAKAGRNAGLLARALAQAPDDAYLHYQIGKDHDVYERWADAALHFNRS
                     RACEAASGATVTLAGTTPGWHHDRLVREMHALKQCKRHADAVLLAEAAMPHWQHSPDF
                     FFALGDLLLDWATEEPARAGELLPMIESAWLRCLEIGERPDLEGSVAGRGSSLAARNL
                     VVLYEGTGQVLKAMKLKQSH"
     gene            1487..2989
                     /locus_tag="VIN75_21765"
     CDS             1487..2989
                     /locus_tag="VIN75_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8976857.1"
                     /translation="MKNAPAIHLAIIQPAGYVHSLGFLDQARYFRHMFRRQGATVTMA
                     KNRLREDAVNVVFGAHLGFPADWQERHACIFVNLEQLGDGGASVSPDYLALLRRSAVV
                     DYDAANVGAYAQDPADVPIVSFGYAAYLDKPALPLEERPIDLLFFGSTNPRRNEFIAR
                     VEAAGFSVAMFDGPVYGPERDEYIRQSKAVLNCHFYESNRFEQARAFHCLSLGTPVIS
                     EYTPTTDPAPAFRDSVLWLPGQVEEWFRLHFKTPAFYEEARKRLAAFRSGDAAHDPME
                     QFADLLAFAEGFRQGFARVRAPGPWRPRAINLGSGRDYKPTWLNIDILARAEPDLVLD
                     LGQPLRLPLSAPTHMGGEVLLEEGALELIHASNVLEHVPDLPLLMTNALRLLKVGGAF
                     SIEVPYEKSLAAWQDPTHLRALNENSWLYYTDWFWYLGWFEHRFEIVKQQWLDAAAKP
                     CGKDAAAFMRVALRKVATTPRERTNARMMRADFGGLDDDQFADADAIALI"
     gene            3003..4502
                     /locus_tag="VIN75_21770"
     CDS             3003..4502
                     /locus_tag="VIN75_21770"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8976858.1"
                     /translation="MNTLAIAMIKNEADIVEAFVRHNLALTDLMVVIDNGSTDGTREI
                     LTSLQREGLPVVVFDDPIFGYYQSEKVTHVYRKVVPVFNPELVWFLDADEFIHAPSRA
                     KLDAAFATVPAGEVVLLPWRTHLPDAQVDPARVLADPLGAMPDRRKREEPQVFKAVVR
                     RNPGDDARLVIDQGNHNVHFTDGTRPPLHVMDGVSIVHLPVRSVEQLSAKVINGWQAY
                     LVKNRHAEVPTAGFQWQLLYERAVYGQGLDAETLTEVALNYAQPERAGRNRRDDAVRD
                     PAPARYGALRYLHQARTNLLAKVAMNMESYILNGDGTWKPETNETAAPRRDIAGLLEV
                     LRQSEAVRLRGAGAEGARWVEELVLANPGWQRTQAGAADMLLAAEMPAPQFAALARDA
                     AAQPTRRVVWWAPQAAAPSELDATLAAWYAAGWEPHMVETLGYRALSAFPELRAGAFV
                     LQRADAAREPRALAVRSALCGMGDGARPAATPVPKRVLHPMQDMELLAA"
     gene            4518..5465
                     /locus_tag="VIN75_21775"
     CDS             4518..5465
                     /locus_tag="VIN75_21775"
                     /inference="COORDINATES: protein motif:HMM:NF013888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976859.1"
                     /translation="MHPLQPLRALSAQAFMAPHTLEERLALAMLTLDRRLFSHDAAGA
                     WLDWIGAHGRELAPEQIEQLMREVLLMSPLHPQAPAIYAALMRPSEPLPAEGRVVFMV
                     TSCRKYFAQAQRVQDDLRARGATACIIIGDPAARAPRWDGDTCTLPVEDNYETLPLKV
                     AAGVNALLQRFGAIAVVKIDDDCHLTANFTPERFLQLSRAHDYVGMLAVNPHLCRFWH
                     FGKTSRPMGPYSRRHHGAWAGGACYLLGPKAAALVASEYAHYPAEIGDEYYEDKAIGD
                     FMRRQGVPLTTIRHADWGITFDTAERFVAPVLEAAVGIA"
     gene            complement(5669..7906)
                     /locus_tag="VIN75_21780"
     CDS             complement(5669..7906)
                     /locus_tag="VIN75_21780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008373 - sialyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 29 protein"
                     /protein_id="HEY8976860.1"
                     /translation="MSQTFSCTEELLQALPQPIAIVGNGVPAGAMGAAIDHHPSVIRL
                     NNYRLDGFESVVGTRTTLRCTSGWNDIEARGQVGEFSPFTRDARESAGVAEFEQRSGF
                     ALICAATDVHALVPGVPNPSTGLALAALCSRLGLQFSLFGFDGFTSGHYWNPGVQHAT
                     THSGGERELLLGLPGAVFYGQSYDYASLYNFCHAEHAGYNVNEGLALYRQLGCVIQHE
                     SILEFGAGNGDLSAYMEKQGNRVTALEVSKVAFNRIPIRSKINGDCITLAGLTQKFDR
                     FVSVDVLEHLTENDIRVVLREAARLADSILVSVSTRLSGLLGPNGENLHLTVRSVEWW
                     RDLFSEHFDVQLVNGLDVGQVMIAGDRRPAGAQRAAPPARPADRFGLPAAYVSRARPV
                     YYADTPDDGLTWQPDVYPTAAEIARSLQCDTLVDIGCGQARKLSVLHPEFRLIGIDYG
                     GNMEHCRTKFPFGTFLEADFEDISMLPLPARVLQRSVVICSDVIEHLVDPRSLMDALR
                     EVLRHAPALVVSTPDRERTWGKAHMGPPPNPAHIREWTLAELAALFEREGFTLAAATH
                     TRSNDGSPDLATIMLVLVNPEHAQIRGSAFAARTAEPRHATPQAAPAAWSAPVASASD
                     LAAASADTPSVTAVPGEVATATAEIVVEGLEETAAAAGEQAMANPTWRARLGRARDDR
                     EMLRVLAQMSLDLGHPDRAMDLYGALLSTDGADHDALEGVVISQIRCGQHADAARLLE
                     SVRSN"
     gene            8276..8599
                     /gene="flhD"
                     /locus_tag="VIN75_21785"
     CDS             8276..8599
                     /gene="flhD"
                     /locus_tag="VIN75_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEY8976861.1"
                     /translation="METNQILAEIREANLSYMMLAQSLIRSDREQALFRLGISEETAN
                     MIASLTPAQMMKIASGNTLLCRFRMDDDLVWNLITSHGKTAANETVSRLHASILMAGR
                     HQEAA"
     gene            8654..9196
                     /gene="flhC"
                     /locus_tag="VIN75_21790"
     CDS             8654..9196
                     /gene="flhC"
                     /locus_tag="VIN75_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEY8976862.1"
                     /translation="MSRVKSILTEARQIERAVTLIKLGARLQVLESETDLSYERLLRL
                     YKEVSGRSPSKGQLPFSTDWFMTWQPNIHASLFLNIHEYLNKAAAMEEIDTVIKAYQL
                     YMEQTEAQGLEPLLSITRAWRLVKFVDNGMLTLTKCTKCSGNFVTHPHEMARHFVCGL
                     CNPPARAGKGKAAGALSLTH"
     gene            9466..>10122
                     /locus_tag="VIN75_21795"
     CDS             9466..>10122
                     /locus_tag="VIN75_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8976863.1"
                     /translation="MARRPGNVLFSTGRPGRSAVAPRLPALRWPLSALLAWAAGWASM
                     LALARTGALPPAQAVAAGLLVAAAPAWLAATFWRRVIVAAGFPASLLATGMAGALPAW
                     AWLLPLAVLALAYPVTAWRDAPMFPTPQGALIGLDHHVALPTSPSLLDAGCGLGHGLR
                     ALRVVWPQARLSGVEWSWPLALAARLRCPWARVARGDMWRASWAAHDLVYLFQRPESM
                     G"
BASE COUNT         1489 a         3716 c         3458 g         1459 t
ORIGIN      
        1 ccgcagcggg gacgactacg tcgagctcgc gacggcgctc gccaacgagc ccgacacgct
       61 gcgcggcatc aaggcccacc tcgaccagaa tcgccgcgtg ctcccgctgt tcgacgccga
      121 gcgcgtggcg cgcgacatgg acgcgctgct gatgcgcatg cacgcgcgcc atctggccgg
      181 cctcgcgccc gcggccctgg cggcggagca ggcatgagca gctacaaggt cgccctcgtc
      241 atgatcgcgc gcaacgaggc cgcgcgcatc gtgcgcgcgc tcgccagcgc cacgaactgc
      301 gtcgacgagc tcgtcgtgct cgacaccgga tccaccgacg ccaccgtcgc actcgcccgc
      361 tccgcgggcg cgcgcgtgca ccacttcaaa tggtgcgacg acttcgccgc cgcgcgcaac
      421 gccgccctgg agctggccga cgccgactgg aacgtcgtgg tcgacgcgga cgagtggatc
      481 gccgaggccg gcatcgagct gctggcgctg cgccacaccg cgcccggctt cgtcggcacg
      541 ctgcgggtgg acagccagtt cgacacgccc atcggcacct ccgtggcccc cagctggctg
      601 ccgcgcgtgc tgccgcgcgg cgtgcgctac gagggccatg tccacgagca gccggagcac
      661 cggctgcccg tgcggcgcct ggcgacggtg ctcgcgcacg acggctaccg cgccgatgcg
      721 ctcaaggcca aggccggccg caacgccggg ctgctcgcgc gcgcgctggc gcaggcgccc
      781 gacgacgcct acctgcacta ccagatcggc aaggaccacg acgtctacga gcgctgggcc
      841 gacgccgcgc tgcacttcaa ccgctcgcgc gcgtgcgagg ccgccagcgg cgccaccgtc
      901 acgctggccg ggacgacgcc gggctggcac cacgaccgcc tggtgcgcga gatgcacgcg
      961 ctcaagcaat gcaagcgcca cgccgacgcg gtgctgctgg ccgaggccgc gatgccgcac
     1021 tggcagcact cgcccgactt cttcttcgcg ctcggcgacc tgctgctgga ctgggccacc
     1081 gaggagccgg cccgcgccgg cgagctgctg ccgatgatcg agtcggcctg gctgcgctgc
     1141 ctggagatcg gcgaacgccc cgacctcgaa ggctccgtcg ccggccgcgg ctcttccctg
     1201 gccgcgcgca acctggtcgt gctctacgaa ggcacgggcc aggtgctcaa ggccatgaaa
     1261 ctgaagcagt cgcactgacg caggggtctg gcctctcctc gaaatcgcgc tcattcgtcg
     1321 tcgacggcca cgacgccggc cgcgattcga ggcgaaggcc agacccctcc ggccccgaat
     1381 tgccggctgg cggcccgtct tttccccgca tggcgcggcc ttcagttctc cagaatcggt
     1441 tcacgaccgt cgatgcggtc cacgaacaat tccggagccc ggttccatga agaatgcccc
     1501 cgcgatccac ctggcgatca tccagcccgc cggctacgtg cactcgctcg gcttcctgga
     1561 ccaggcgcgc tacttccgcc acatgttccg ccgccagggc gccacggtga ccatggccaa
     1621 gaaccggctg cgcgaggacg ccgtgaacgt cgtcttcggc gcgcacctgg gctttcccgc
     1681 cgactggcag gagcgccacg cctgcatctt cgtcaacctc gagcagctcg gcgacggcgg
     1741 cgcgtccgtc agcccggact acctcgcgct gctgcgccgc tcggcggtgg tcgactacga
     1801 cgccgccaac gtcggcgcct acgcgcagga tccggccgac gtgcccatcg tgtcgttcgg
     1861 ctacgccgcc tacctcgaca agcccgcgct gccgttggaa gagcgtccga tcgacctgct
     1921 gttcttcggc agcaccaacc cgcgccgcaa cgagttcatc gcccgcgtgg aggccgccgg
     1981 cttcagcgtg gccatgttcg acggcccggt gtacggcccc gagcgcgacg agtacatccg
     2041 ccagtccaag gcggtgctga actgccactt ctacgagagc aaccgcttcg agcaggcgcg
     2101 cgcgttccac tgcctgtcgc tgggcacgcc cgtgatctcg gagtacacgc ccaccaccga
     2161 tccggcgccc gcgttccgcg actcggtgct gtggctgccc ggccaggtcg aggaatggtt
     2221 ccgcctgcac ttcaagacgc ccgcgttcta cgaggaggcg cgcaagcgcc tggccgcgtt
     2281 ccgctcgggc gacgccgcgc acgacccgat ggagcagttc gccgacctgc tggccttcgc
     2341 ggagggcttc cgccagggct tcgcgcgcgt acgcgcgccg ggcccgtggc gcccgcgcgc
     2401 gatcaacctc ggctcgggcc gggactacaa gccgacctgg ctcaacatcg acatcctcgc
     2461 ccgcgccgag cccgacctcg tgctcgatct cggccagccg ctgcgactgc cgctgtcggc
     2521 acccacgcac atgggcggcg aggtgctgct cgaggagggc gcgctggagc tgatccatgc
     2581 gagcaacgtg ctggagcacg tgcccgacct gccgctgctg atgaccaacg cgctgcgcct
     2641 gctcaaggtg ggcggcgcgt tctccatcga ggtgccgtac gagaagtcgc tcgccgcctg
     2701 gcaggatccg acgcacctgc gcgcgctcaa cgagaactcg tggctctact acaccgactg
     2761 gttctggtac ctgggctggt tcgagcaccg cttcgagatc gtcaagcagc agtggctcga
     2821 tgccgccgcc aaaccgtgcg gcaaggacgc cgccgcgttc atgcgcgtgg cgctgcgcaa
     2881 ggtcgccacc acaccgcgcg agcgcaccaa cgcccgcatg atgcgcgccg acttcggcgg
     2941 cctggacgac gaccagttcg ccgacgccga cgccatcgcc ctgatctgac cgggacaccg
     3001 acatgaacac cctcgccatc gcgatgatca agaacgaggc cgacatcgtc gaggccttcg
     3061 tccgccacaa cctggccctg acggacctga tggtcgtcat cgacaacggc tcgaccgacg
     3121 gcacgcgcga gatcctgacc tcgctgcagc gcgaaggcct gcccgtcgtg gtcttcgacg
     3181 acccgatctt cggctactac cagtccgaga aggtgacgca cgtctatcgc aaggtcgtgc
     3241 ccgtcttcaa cccggagctc gtgtggttcc tggacgccga cgagttcatc cacgcgccgt
     3301 cgcgcgcgaa gctggacgcc gcgttcgcga cggtgcccgc cggcgaggtg gtgctgctgc
     3361 catggcgcac ccacctgccc gacgcgcagg tcgacccggc ccgcgtgctg gccgacccgc
     3421 tgggcgcgat gcccgaccgc cgcaagcgcg aggagccgca ggtcttcaag gcggtggtgc
     3481 gccgcaaccc gggcgacgac gcgcgcctgg tcatcgacca gggcaaccac aacgtccact
     3541 tcaccgacgg cacgcgcccg ccgctgcacg tgatggacgg cgtcagcatc gtccacctgc
     3601 cggtgcgcag cgtcgagcag ctgtcggcca aggtgatcaa cggctggcag gcctacctcg
     3661 tgaagaaccg ccatgccgag gtgcccaccg cgggcttcca gtggcagctg ctgtacgagc
     3721 gcgccgtcta cggccagggc ctcgacgccg agaccctgac cgaggtcgcg ctgaactacg
     3781 cgcagcccga gcgcgccggc cgcaaccgcc gcgacgacgc ggtacgcgac ccggccccgg
     3841 cgcgctacgg cgcgctgcgc tacctgcacc aggcgcgcac gaacctgctg gccaaggtcg
     3901 cgatgaacat ggagtcgtac atcctcaacg gcgacggcac ctggaagccc gagaccaacg
     3961 agaccgccgc gccgcggcgc gacatcgcgg gcctgctgga ggtgctgcgc cagtccgagg
     4021 cggtgcgcct gcgcggcgcc ggcgccgagg gggcgcgctg ggtcgaggaa ctcgtgctgg
     4081 ccaacccggg ctggcaacgc acccaggcgg gcgccgccga catgctgctg gccgccgaga
     4141 tgcccgcgcc gcagttcgcc gcgctcgcac gcgacgccgc ggcgcagccg acacgccgcg
     4201 tcgtctggtg ggcgccgcag gccgcggccc cgtccgaact cgatgccacg ctcgccgcct
     4261 ggtatgcggc cggctgggag ccgcacatgg tcgagacgct gggttatcgc gccctgtcgg
     4321 ccttccccga gctgcgcgcc ggcgccttcg tgctgcagcg cgccgacgcg gcccgcgagc
     4381 cgcgcgcgct ggccgtgcgc agcgccctgt gcggcatggg cgacggggcg cgccccgcgg
     4441 ccacgccggt gcccaagcgc gtcctgcatc cgatgcagga catggaactg ctggccgcct
     4501 gagtccccga ggtcgacatg catcccctgc aaccgctgcg cgccctgtcc gcgcaggcct
     4561 tcatggcccc gcacacgctc gaggagcgcc tcgcgctcgc gatgctcacg ctggaccggc
     4621 gcctgttcag ccacgacgcc gccggtgcgt ggctggactg gatcggcgcg cacggccgcg
     4681 agctcgcacc cgagcagatc gagcagctga tgcgcgaggt gctgctgatg tcgccgctgc
     4741 atccgcaggc cccggccatc tacgccgcgc tgatgcgccc gagcgagccg ctgcccgccg
     4801 agggccgcgt cgtgttcatg gtgaccagct gccgcaagta cttcgcacag gcgcagcgcg
     4861 tgcaggacga cctgcgcgcc cgcggcgcca ccgcctgcat catcatcggc gaccccgccg
     4921 cgcgcgcgcc gcgctgggac ggcgacacct gcacgctgcc ggtcgaggac aactacgaga
     4981 cgctgccgct gaaggtggcc gccggcgtca acgcgctgct gcaacgcttc ggcgcgatcg
     5041 ccgtcgtcaa gatcgacgac gactgccacc tgacggccaa cttcacgccc gagcgcttcc
     5101 tgcagctgtc ccgcgcccac gactacgtcg gcatgctggc cgtcaacccg cacctgtgcc
     5161 gcttctggca cttcggcaag acctcgcggc cgatgggccc gtactcgcgc cgccaccacg
     5221 gcgcctgggc cggcggcgcc tgctacctgc tcggcccgaa ggccgccgcg ctggtggcca
     5281 gcgagtacgc gcactacccc gccgagatcg gcgacgagta ctacgaggac aaggccatcg
     5341 gcgacttcat gcgccggcag ggcgtgccgc tcaccacgat ccgccacgcc gactggggca
     5401 tcaccttcga caccgccgag cgcttcgtgg cgccggtgct cgaggccgcc gtcggcatcg
     5461 cctgacagcc ggcatcggga tcgcccgccg ggcacgccgg cgccggcgct gtcgtgaccg
     5521 gcgcgggcca tggtcagcga gcgatgcctg gcgacaccgg cgtgctcgaa cctgtgcggc
     5581 cgcccgcgct tggccgcgat gacaaaggcc cggcatctca cgacaccggg cctcctgatt
     5641 ccgggacgca ggccatgcct gccgccgctc agttcgaccg cacggattcc agcaggcggg
     5701 ccgcatcggc gtgctggccg cagcggatct gcgagatgac cacgccttcg agggcgtcgt
     5761 ggtcggcgcc gtcggtcgac agcagggcgc cgtacaggtc catcgcgcgg tcggggtggc
     5821 cgaggtccag gctcatctgg gccaggacac gcagcatctc gcggtcgtcg cgggcgcggc
     5881 cgaggcgggc gcgccaggtc gggttggcca tggcctgctc gccggcggcg gcggccgtct
     5941 cttccagccc ttcgacgacg atctcggccg tggcggtggc gacctcgccc gggacggccg
     6001 tcacggacgg cgtgtcggcc gaggccgctg cgaggtcgga cgcggacgcg accggcgcgc
     6061 tccaggcggc cggggcggcc tgcggcgtcg cgtgacgcgg ctcggcggtt cgggccgcga
     6121 acgcgctgcc gcggatctgc gcatgctcgg ggttgaccag caccagcatg atcgtcgcca
     6181 ggtccgggct gccgtcgttg ctgcgcgtgt gcgtggccgc ggccagcgtg aagccttcgc
     6241 gttcgaacag cgcggcgagc tcggccagcg tccattcgcg gatgtgggcc gggttgggcg
     6301 gcgggcccat gtgggccttg ccccaggtgc gctcgcggtc gggcgtggag acgaccagcg
     6361 ccggcgcgtg gcgcagcacc tcgcgcaaag cgtccatcag gctgcgcggg tcgaccaggt
     6421 gctcgatgac gtccgagcag atgacgaccg agcgctgcag cacgcgcgcg ggcagcggca
     6481 gcatcgagat gtcctcgaag tcggcttcca ggaaggtgcc gaacgggaac ttcgtgcggc
     6541 agtgttccat gttgccgccg tagtcgatgc cgatcagcct gaactcgggg tgcagcacgc
     6601 tgagcttgcg cgcctggccg cagccgatgt cgacgagcgt gtcgcattgg agcgagcgcg
     6661 cgatctcggc ggcggtcggg tagacgtcgg gctgccaggt caggccgtcg tcgggcgtgt
     6721 cggcgtagta gacgggcctg gcgcgcgaca cgtaggccgc gggcaggccg aagcggtcgg
     6781 cagggcgcgc gggcggggcg gcgcgctggg cgcccgccgg gcggcggtcg ccggcgatca
     6841 tcacctggcc gacgtccagg ccgttgacca gctgcacgtc gaagtgctcg gagaacaggt
     6901 cgcgccacca ttcgacggag cgcaccgtca ggtgcaggtt ctcgccgttg gggcccagca
     6961 ggccgctcag gcgcgtggac accgacacca ggatcgagtc cgccaggcgg gccgcctcgc
     7021 gcagcacgac gcggatgtcg ttctccgtca gatgctccag cacgtccacc gacacgaagc
     7081 ggtcgaactt ctgcgtcagg ccggccagcg tgatgcagtc gccgttgatc ttgctgcgga
     7141 tggggatgcg gttgaacgcg accttggaga cctcgagcgc cgtcacgcgg ttgccctgct
     7201 tctccatgta ggccgacagg tcgccgttgc cggcgccgaa ctcgaggatg gactcatgct
     7261 ggatgacgca gccgagctgg cgatacagcg ccaggccctc gttgacgttg tagccggcgt
     7321 gctcggcgtg gcagaagttg tacaggctcg cgtagtcgta gctctggccg tagaacaccg
     7381 cgccgggcag gcccagcagc agctcgcgct cgccgccgct gtgcgtggtg gcgtgctgca
     7441 cgccgggatt ccagtagtgg ccgctcgtga agccgtcgaa gccgaacagg ctgaactgca
     7501 ggcccagccg gctgcacagc gcggccagcg cgaggccggt ggacggattg ggcacgcccg
     7561 gcaccagcgc gtgcacgtcg gtggcggcgc agatcagcgc gaagccgctg cgctgctcga
     7621 actcggcgac gccggcggac tcgcgcgcgt cgcgggtgaa cggggagaac tcgccgacct
     7681 ggccgcgcgc ctcgatgtcg ttccagccgg aggtgcagcg cagcgtcgtg cgggtgccga
     7741 cgacggactc gaagccgtcg agccggtagt tgttgagccg gatcacgctg ggatggtgat
     7801 cgatggcggc gcccatggcc cccgcgggga cgccgttgcc gacgatggcg atcggctggg
     7861 gcagcgcctg cagcagctct tcggtgcacg agaaggtttg gctcatgggc tctccggttc
     7921 gggagtggac gaccgatgga acgatcgcca cgggaatgcg cccatgctat gaggcccttg
     7981 ctgcgtccaa agcgggaaga agtgggccct tttcggggtt aatgagtgcc gcgcggcgcc
     8041 gaccggaggt cacgcaggcg cccagccgtc gagctgcgcg ccacggatcg atcgaccgtc
     8101 cagggcggca atcggcccgc acctcggagt ttgtaggaat ttgcctgaca acgttcctgg
     8161 cgcgcgcccg actcaagtcg ccgaagtggg cttatgttgc ctcccagttc gtcgctttaa
     8221 agtccatctc acgttgacac cgtttcttcc tccaccggac acaaggacaa gcatcatgga
     8281 aaccaatcaa atcctcgccg agatccgcga agccaacctg tcgtacatga tgctggcgca
     8341 aagcctgatc cgctcggatc gcgaacaggc cctgttccgc ctgggcatct cggaagagac
     8401 ggccaacatg atcgcctcgc tgaccccggc gcagatgatg aagatcgcct cgggcaacac
     8461 gctgctgtgc cgcttccgca tggacgacga cctggtctgg aacctgatca ccagccacgg
     8521 caagaccgct gccaacgaga ccgtctcgcg cctgcacgcc tccatcctga tggccggccg
     8581 ccaccaggaa gccgcgtaag ccgcctcgcg ccgcaacccg acagcacaac gaacccgaag
     8641 gcaggagatc accatgagcc gcgtcaagag catcctcacc gaagcccgtc agatcgaacg
     8701 cgccgtcacg ctgatcaagc tcggcgcccg cctgcaggtt ctggagtcgg aaaccgacct
     8761 gtcctacgag cgcctgctgc gcctgtacaa ggaagtctcg ggccgcagcc cgtccaaggg
     8821 ccagctgccc ttctcgacgg actggttcat gacctggcaa cccaacatcc acgccagcct
     8881 cttcctgaac atccacgagt acctgaacaa ggccgcggcg atggaagaga tcgacacggt
     8941 gatcaaggcc taccagctct acatggagca gaccgaggcc cagggcctgg agccgctgct
     9001 gtccatcacc cgcgcctggc gcctggtgaa gttcgtcgac aacggcatgc tgacgctgac
     9061 caagtgcacc aagtgcagcg gcaacttcgt cacccatccg cacgagatgg cgcgccattt
     9121 cgtctgcggc ctgtgcaacc cgccggcgcg cgccggcaag ggcaaggcgg ccggcgccct
     9181 gtccctgacc cactgaacga ccgatccgcg tcatttcggc gtcgacgtgc ggtagtcgag
     9241 catccccgac agggatgcac accgcgagcc gtgaactagt acgcgattct ttacagaccc
     9301 gggcttgatt ggtggaacaa accgcccgat atgaggtcta gtagttactg ttttttcttg
     9361 cttgtctcct cctagcacaa cccctgtgct ttccaaggcg atcccgaacc ggatcgcctt
     9421 tttctttgtg ggccgacgtg agccccccgc tgttgcgcaa cacgcgtggc aagacggccg
     9481 ggaaacgtcc tcttttccac cgggcgccca ggccgttccg ccgtcgcgcc gcgcctgccc
     9541 gcgctgcgct ggccgctgtc cgcgctgctc gcgtgggcgg ccggctgggc gtcgatgctg
     9601 gcgctcgcgc gcacgggtgc gctgccgccg gcgcaggccg tcgccgccgg gctgctggtc
     9661 gccgccgcgc cggcctggct cgccgccacg ttctggcgcc gggtgatcgt cgcggccgga
     9721 ttccccgcgt cgctgctggc cacgggcatg gccggcgcgc tgccggcctg ggcctggttg
     9781 ctgccgctgg ccgtgctggc gctcgcctac cccgtcaccg cctggcgcga cgcgccgatg
     9841 ttcccgacgc cgcagggcgc gctgatcggc ctggaccacc acgtcgcgct gccgacatcg
     9901 cccagcctgc tggacgccgg ctgcggactc ggccacggcc tgcgcgcgct gcgcgtcgtc
     9961 tggccgcagg cccggctgtc gggcgtcgaa tggagctggc cgctcgcgct cgcggcacgc
    10021 ctgcgctgcc cgtgggcgcg ggtcgcgcgc ggcgacatgt ggcgcgcctc ctgggccgcg
    10081 cacgacctgg tctacctgtt ccagcgcccg gagagcatgg ga
//
LOCUS       DATCRO010000800         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_650369, whole genome shotgun sequence.
ACCESSION   DATCRO010000800 DATCRO010000000
VERSION     DATCRO010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650369"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1464)
                     /locus_tag="VIN75_21800"
     CDS             complement(136..1464)
                     /locus_tag="VIN75_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8976864.1"
                     /translation="MQSQSVLPADAAPLPEVEKPALKRSPITWVPTLYFAQGVPFFVV
                     MAIAALLFKDLGIENSVITKWTNALGLAWAVKPLWSPFLEVVPSKKLLVIVFQLVGAA
                     TLGLVALALNVPHSVSLVLAILAFTAFGSATHDISSDGLYIASLNDKQQAEYAGWQGA
                     FFNAARLFARGGVLVLVGYLEKSMEAAHAWSSVFVLLAAVMAALALYHVWALPDTRTG
                     VAASRDVGAIARTTAEVVVEFFRKPGIWFAILFIILFRAGEGQIQSVGPLFLKDARAA
                     GGLGLTTDQIGVVYGTWATVAFVGGSIVGGYFTSWLGLRRSMVWLVIAMNLPNLVYWW
                     LGTARPSDMSLIATGLSIEMFGYGFGFVGIILFIMQVVAKGRFTTAHYALGTGVMQLG
                     FILSGTWSGQIQTWLGYQNFFLWVLACAVPVLVLSFFLPHHDDASAAKTA"
     gene            complement(1543..3063)
                     /gene="nagZ"
                     /locus_tag="VIN75_21805"
     CDS             complement(1543..3063)
                     /gene="nagZ"
                     /locus_tag="VIN75_21805"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY8976865.1"
                     /translation="MSTSFHPGHLVMVDIPGTSLDAETAEFLRRHRVRAICLFRRNLG
                     TEDEIRKLTADLREVMGPHSLIGIDQEGGSTIRATFLPQAPAAMALGAIGDEALCEEV
                     GAVVARGLRSLGINWNFAPVLDVNNNPGNPVIAERSFSEDPDEVARLAAAWMRGSLRE
                     GVACCVKHFPGHGDTATDSHHALPVVDKSVAQLEALELKPFRALAHARGATPAAPSVM
                     TAHIVYPQIDPEHPATLSRPVLTGLLRENLGYDGVVITDALMMKAVHDKYGHAVGAVM
                     ALQAGADMPLAQGTLAEQAATVKAIEQALGEGHLLPADVKKSEARLVALAQAYPVSPK
                     AYDAAQRAADDAAMRAAWARGLTAFDGAGKPATPPAAGTPLRVVTQDSVECDGVSEAG
                     LPAGRVMEMFAGFPDVQLVKVPDLRAIDASSFPRDGRFNVLVSNHRTRFGAAAGDARP
                     DLHVALWNPFQLFDIAAPSLVTWGYCDGALEAVRAWLEGRAQATATSPVNLSPRPA"
     gene            complement(3081..4091)
                     /locus_tag="VIN75_21810"
     CDS             complement(3081..4091)
                     /locus_tag="VIN75_21810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8976866.1"
                     /translation="MFDEAREAPAAVARLMAADLGAYTSLGEQLRADPPRSALTVARG
                     SSDHAAHYMAYLVMARLGRLVTSLPMSLVTLYQSQLDCDGLVSWGFSQSGQSPDLIAP
                     TRFFREGGARTVAVVNDAGSPLAEAAQWVLPLHAGAETSVAATKSYIAQLVAGARIVA
                     HWQNDQALLSALETLPEVLERAAYADWSPLVEALVDADKLFVIGRGTSLAIAMEAALK
                     FKEVCGIQAEAFSGAEVKHGPMALIDEGYPLLVFAPRGPAQAGLLQLADDMRRRGARV
                     LVAAPVGTPGAELPLVVAGHEDLDPIAAIQSYYLAVEALARARGLDPDKPRHLNKVTL
                     TH"
     gene            complement(4084..5217)
                     /gene="nagA"
                     /locus_tag="VIN75_21815"
     CDS             complement(4084..5217)
                     /gene="nagA"
                     /locus_tag="VIN75_21815"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY8976867.1"
                     /translation="MTVSRKQLAGHVLTPQGFIRGTLSVVDGRIAGIEGTPISEGQAR
                     VSAEPLILPGFIDLHVHGGGGFDTMEGGNAVAQIARIHARHGTTSMLATTMTAPMADI
                     TQAMTALADACRTHRRGGAHVLGVHLEGPYINPGALGAQPDYARPASVDEIKALHAIA
                     PIRLITMAPELPGNLDLIAPLRAAGFHVQIGHSAGTYEDGVAALQRGATGFTHLFNAM
                     SKFHHREPGIAGAALAHAEFAEIIPDLLHVHPGAIRAALRAIPCLYCVTDSTAAAGMP
                     DGDYNLGSHRVTKCLGGVRLADGTLAGSTLTMDQALRNLVGMGLSLDDASRRVSTNAA
                     DFLGLADRGRLFVGARADLVVLDAGLQLTQVFVEGDSIELTDV"
     gene            complement(5324..6241)
                     /locus_tag="VIN75_21820"
     CDS             complement(5324..6241)
                     /locus_tag="VIN75_21820"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY8976868.1"
                     /translation="MSHTCYHPAFEQADIRYALGVDGGGTGTRVRVWTNAGALVGHGE
                     SGPSALAQGVDQAWRHIGQAVERAFASAGLAMPAPSHCVLGLGLAGAVAPHLAAAFFR
                     AAPEYALIVLETDSRTALMGAHAGRPGAIIAVGTGAVGEALHADGRRVAVSGWGFPVG
                     DEGSGAWLGLRAMQIAHRALDGRAPAGTLAQAVWRQVSDRRDTLLEWCSGAGQHEYAQ
                     LAPLVFECANSDAAAQSLVQEIGQEIEDMALALDPGLQLPIVLLGSIGLRMAPNLAPA
                     LRARIVEASGDAVDGAHRLVRQQMKEAVA"
     gene            complement(6452..6940)
                     /locus_tag="VIN75_21825"
     CDS             complement(6452..6940)
                     /locus_tag="VIN75_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8976869.1"
                     /translation="MDTSPPPAKRYYWEDFPAGRVIPLGSRAVTRDETLAFARQFDPQ
                     PFHLDDAAAEASLFKRLSASGWHTCAMTMRLMCDGYLNDSAGLGSPGVDELRWLKPVY
                     PGDTISAEMTVLESRVMKSKPHVGLVLSRWRVFNQDGEDVMTMQGWGMFALRHPAPPV
                     AG"
     gene            complement(7061..7786)
                     /locus_tag="VIN75_21830"
     CDS             complement(7061..7786)
                     /locus_tag="VIN75_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8976870.1"
                     /translation="MTGGPAIHSLFWLAVTRLGEAQILLPAFLAGALWLAFARPAGAR
                     GRLAQGNPHAHDHPARRSALRWVAGITATTAVTTASKIAFLGFGYGIAAIDFTGFSGH
                     SMYAWSILPVLGAIVAGRRGVAVGIALALLITYSRVDLGAHSWSEAIAGTALGVAASG
                     WTLADYLAHPGAVRAPWWLPLLIVAWLTVLPTKAPPSRTHSLVVSISLKLSGRARPYT
                     RFELLAGLIHPAWEAPPGAGSSR"
     gene            complement(8090..>8746)
                     /locus_tag="VIN75_21835"
     CDS             complement(8090..>8746)
                     /locus_tag="VIN75_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8976871.1"
                     /translation="VVLATGVKARDPRIPGQEHPKVLSYIDVLLHGKPVGERVAIVGA
                     GGIGFDVAEFLVTGADRGEPSATLHLDEWKREWGVGDPARTPGGLDRPQPSAPARQVT
                     LLQRKAGKHGAGLGKTSGWVHRATLKMKNVEMLGGVNYERVTDDGLWVTFGPERKDGT
                     LVAADTIVLCAGQEPLRELAGPLAAAGIKVHLIGGADEASELDAKRAINQGTRLAAAI
                     "
BASE COUNT         1289 a         3046 c         3218 g         1193 t
ORIGIN      
        1 tcgtcgaaga acagctgcgc gggtgcgacg gtcttcgatg cggagacatc ggcgcaacca
       61 gcaatggcgg agacgacggt gaacgaggcg atggcccggc ccgacgtcag ccacatgaac
      121 gacggacggc gcatttcagg cggtcttggc cgccgacgcg tcgtcatgat gcggcaggaa
      181 gaacgagagc acgagcacgg gcacggcgca ggccagcacc cacaggaaga agttctggta
      241 gccgagccag gtctggatct ggccgctcca ggtgcccgag aggatgaagc ccagctgcat
      301 cacacccgtg ccgagcgcat agtgcgccgt ggtgaagcgg cccttggcga ccacctgcat
      361 gatgaacagg atgatgccga cgaagccgaa gccgtagccg aacatctcga tcgacaggcc
      421 ggtcgcgatc aggctcatgt cgctcgggcg cgcggtgccg agccaccagt agacgaggtt
      481 gggcaggttc atcgcgatga ccagccacac catcgaccgg cgcaggccca gccaagacgt
      541 gaagtagccg ccgacgatgc tgccgcccac gaaggccacc gtggcccagg tgccgtagac
      601 cacgccgatc tggtcggtcg tcagcccgag gccgccggcc gcgcgcgcgt ccttcaggaa
      661 cagcggcccg accgactgga tctggccttc gccggcgcgg aacaggatga tgaacaggat
      721 cgcgaaccag atgccgggct tgcggaagaa ctcgacgacc acctccgccg tggtcctcgc
      781 gatcgcaccc acgtcgcgcg acgcggcgac gccggtgcgc gtatcgggca gcgcccacac
      841 gtggtacagc gccagcgccg ccatcaccgc agcgagcagg acgaacaccg agctccaggc
      901 gtgcgcggcc tccatcgact tctcgagata gcccaccagc accagcacgc cgccgcgcgc
      961 gaacaggcgc gccgcgttga agaacgcgcc ctgccagccc gcgtactccg cctgctgctt
     1021 gtcgttgagg ctggcgatgt acaggccgtc cgacgagatg tcgtgcgtgg cggagccgaa
     1081 tgcggtgaac gcgaggatgg ccagcaccag cgacacgctg tgcgggacgt tgagcgccag
     1141 cgcgaccagg cccagcgtcg cggcgcccac cagctggaac acgatcacca gcagcttctt
     1201 gctcggcacg acctccagga acgggctcca cagcggcttg acggcccacg ccaggcccag
     1261 ggcgttcgtc cacttggtga tcacgctgtt ctcgatgccg agatccttga acagcagtgc
     1321 cgcgatcgcc atcaccacga agaacggcac gccttgtgcg aagtacagcg tcggcaccca
     1381 cgtgatcggc gagcgcttga gcgccggctt ctccacttcg ggcagcggcg cggcatcggc
     1441 gggcaggacg gattggcttt gcacgggggc tctctttccg ggcggcgatg gcgcgcgata
     1501 ggtcacgggt cgactcgggg tcgtgcggca gtgtgcccgc gttcaggcgg ggcgtggaga
     1561 gaggttcacc ggagacgtcg cggtggcctg cgcgcggcct tcgagccatg cgcgcacggc
     1621 ctcgagcgcg ccgtcgcagt agccccaggt caccagcgac ggggccgcga tgtcgaacag
     1681 ctggaacgga ttccacagcg cgacgtgcag gtcgggccgc gcatcgccgg ccgcggcgcc
     1741 gaagcgcgtg cggtggttcg agacgagcac gttgaagcgg ccgtcgcgcg ggaagctgga
     1801 cgcgtcgatc gcgcgcaggt cgggcacctt gacgagctgc acgtcgggaa agccggcgaa
     1861 catctccatc acgcgcccgg ccggcaggcc ggcctcggag acgccgtcgc attccaccga
     1921 gtcctgcgtg acgacgcgca gcggcgtgcc ggccgcgggt ggcgtcgcgg gcttgcccgc
     1981 gccgtcgaac gcggtcaggc cgcgcgccca cgcggcgcgc atggcggcgt cgtcggcggc
     2041 gcgctgcgcc gcgtcgtagg ccttgggcga gacgggatag gcctgcgcga gcgcgaccag
     2101 gcgcgcctcg gacttcttga cgtccgccgg cagcaggtgg ccttcgccca gcgcctgctc
     2161 gatcgccttg accgtcgcgg cctgctcggc gagcgtgccc tgcgcgagcg gcatgtcggc
     2221 cccggcctgc agcgccatca cggcgccgac ggcgtggccg tacttgtcgt gcacggcctt
     2281 catcatcagc gcgtcggtga tgacgacgcc gtcgtagccg aggttctcgc gcagcaggcc
     2341 cgtgagcacc gggcgtgaca gcgtggcagg atgctcgggg tcgatctgcg ggtagacgat
     2401 gtgcgcggtc atcaccgacg gcgcggccgg cgtggcgccg cgcgcatgcg ccagtgcgcg
     2461 gaacggcttc aactccagcg cctcgagctg cgcgacggac ttgtcgacga cgggcagcgc
     2521 atggtgcgaa tcggtggcgg tgtcgccgtg gccggggaag tgcttgacgc agcacgccac
     2581 gccttcgcgc aacgagccgc gcatccacgc ggcggccagg cgcgcgacct cgtccgggtc
     2641 ctcgctgaag ctgcgctccg cgatgacggg attgccaggg ttgttgttga cgtcgaggac
     2701 cggcgcgaag ttccagttga tgcccaggct gcgcaggcca cgcgcgacga cggcgccgac
     2761 ttcctcgcac agcgcctcgt cgccgatcgc ccccagcgcc atcgcggcgg gcgcctgcgg
     2821 caggaacgtc gcgcggatcg tcgagccgcc ttcctggtcg atgccgatca gcgagtgcgg
     2881 gcccatcacc tcgcgcaggt cggccgtgag cttgcggatc tcgtcctcgg tgccgaggtt
     2941 gcggcggaac aggcagatcg cgcgcacccg gtggcggcgc aggaactccg cggtctcggc
     3001 gtcgagcgac gtgcccggga tgtcgaccat cacgagatgg ccaggatgga aggacgtgga
     3061 cacggcgctg tctcgggcga tcagtgagtg agcgtgacct tgttcaggtg gcgcggcttg
     3121 tcggggtcga gcccgcgcgc acgcgcgagc gcctcgacgg ccaggtagta gctctggatc
     3181 gcggcgatgg ggtcgaggtc ctcgtggccc gcgaccacca gcggcagctc ggcgcccggc
     3241 gtgcccaccg gcgcggcgac gagcacgcgc gcgccgcggc ggcgcatgtc gtcggccagt
     3301 tgcagcaggc cggcctgggc cgggccgcgc ggcgcgaaca cgagcagcgg gtagccctcg
     3361 tcgatcagcg ccatcgggcc gtgcttgacc tcggcgcccg agaacgcctc ggcctggatg
     3421 ccgcagactt ccttgaactt gagcgcggct tccatcgcga tggccaggct ggtgccgcgg
     3481 ccgatcacga acagcttgtc ggcgtcgacc agcgcctcga ccagcggtga ccagtcggcg
     3541 tacgccgcgc gttccagcac ctcgggcagc gtctcgagcg cggacagcag cgcctggtcg
     3601 ttctgccagt gcgcgacgat gcgcgcgccg gccacgagct gggcgatgta gctcttggtg
     3661 gcggccacgc tggtctcggc gccggcgtgc agcggcagca cccattgcgc ggcctcggcc
     3721 agcggcgagc cggcgtcgtt gacgacggcc acggtgcgcg cgccgccttc gcggaagaaa
     3781 cgcgtcggcg cgatcaggtc ggggctctgg cccgactgcg agaagcccca ggacaccagc
     3841 ccgtcgcaat cgagctgcga ctggtacagc gtgacgagcg acatcggcag cgacgtgacg
     3901 aggcgcccca ggcgcgccat cacgaggtag gccatgtagt gcgcggcgtg gtcggagctg
     3961 ccacgggcga ccgtcagggc ggaccgcggc ggatcggcac ggagttgctc gccaagcgag
     4021 gtgtaggcgc caaggtcggc cgccatcaga cgagccacgg ccgcgggggc ctcgcgggct
     4081 tcgtcaaaca tccgtgagct caatggaatc tccttctacg aacacttggg tcagttgcag
     4141 gccggcgtcg agcacgacca gatcggcgcg cgcgccgacg aacaggcggc cgcgatcggc
     4201 cagcccgagg aaatcggccg cgttggtgga cacgcggcgc gacgcgtcgt ccaggctcag
     4261 gcccatgccg acgaggttgc gaagggcctg gtccatcgtc agcgtgctgc cggccagcgt
     4321 gccgtcggcc aggcgcacgc cgcccaggca cttggtgacg cggtggctgc cgaggttgta
     4381 gtcgccgtcg ggcatgccgg cggcggccgt cgaatcggtg acgcagtaca ggcaggggat
     4441 cgcgcgcagc gcggcgcgga tggcgccggg atgcacgtgc agcaggtcgg ggatgatctc
     4501 ggcgaactcg gcatgcgcca gcgccgcgcc ggcgatgccg ggctcgcggt ggtggaactt
     4561 gctcatcgcg ttgaacaggt gcgtgaagcc cgtggcgccg cgctgcaggg cggcgacgcc
     4621 gtcctcgtag gtccccgccg agtggccgat ctgcacgtgg aagccggccg cgcgcagcgg
     4681 cgcgatcagg tcgaggttgc cgggcagctc aggcgccatc gtgatcaggc ggatcggcgc
     4741 gatggcgtgc agcgccttga tctcgtcgac cgacgcgggc cgcgcgtagt ccggttgcgc
     4801 gccgagcgcg cccgggttga tgtacgggcc ttcgaggtgc acgcccagca cgtgggcacc
     4861 gccgcggcgg tgcgtgcggc aggcgtcggc cagcgcggtc atcgcctgcg tgatgtcggc
     4921 catcggggcc gtcatcgtcg tggcgagcat cgacgtcgtg ccgtggcggg cgtggatgcg
     4981 cgcgatctgg gcgacggcgt tgccgccttc catggtgtcg aagccgccgc cgccgtgcac
     5041 gtgcaggtcg atgaagcccg gcaggatcag cggctcggcc gacacgcggg cctggccttc
     5101 gctgatgggc gtgccctcga tgccggcgat gcggccgtcg acgacgctca gcgtgccgcg
     5161 gatgaagcct tgaggcgtca ggacgtggcc ggccagctgc ttgcgcgaca cggtcattgg
     5221 agcccctccc gcgtcgggta cgccgctccg tcccccgagg ggactgcggt ccggcttgtg
     5281 gcggcccggc gcgcgtccag cagacaggtt gacaccggac acttcatgcc accgcttcct
     5341 tcatctgctg gcgcacgagc cggtgcgcgc cgtcgacggc gtcgccggag gcctcgacga
     5401 tgcgcgcgcg cagcgccggc gccaggttcg gggccatgcg caggccgatg ctgcccagca
     5461 gcacgatggg caactgcagc ccggggtcga gcgcgagcgc catgtcctcg atctcctgtc
     5521 cgatctcctg caccagcgac tgcgccgcgg catcggagtt cgcgcactcg aacacgagcg
     5581 gcgcgagttg ggcgtattca tgctggccgg caccggagca ccattccagc agggtgtcgc
     5641 ggcggtcgct gacctggcgc cagacggcct gggccagcgt gccggccggg gcgcgaccgt
     5701 cgagcgcgcg gtgcgcgatc tgcatggcgc gcaggcccag ccaggcgccg gagccttcgt
     5761 cccccaccgg gaagccccag ccgctgaccg ccacgcggcg gccgtcggcg tgcagcgcct
     5821 cgcccacggc gccggtgccc acggcgatga tggcgcccgg acggccggcg tgcgcgccca
     5881 tcagcgcggt gcgcgaatcg gtctccagga cgatcagggc atattcgggc gccgcgcgga
     5941 agaacgcggc agccaggtgc ggcgccaccg cgcccgccag gcccaggccc agcacgcagt
     6001 ggctcggggc gggcatggcc agcccggccg aggcgaacgc ccgctcgacc gcctggccga
     6061 tgtgccgcca cgcctggtcg accccctggg ccagcgccga ggggccggac tcgccgtggc
     6121 ccaccagggc gccggcgttc gtccagaccc ggacacgggt gcccgttccg ccaccgtcca
     6181 cgcccagcgc gtagcggatg tcggcctgtt cgaaggcggg gtgatagcag gtgtggctca
     6241 taagtgtgat accactatag taccatgaga gaccgctgtc aacgaacact cgtccgcgag
     6301 ggatggcgcg gggccgcggc cgtttcactg gtgaaatcta ggtaatgacc cgtacattgt
     6361 tgggcaacga ggtgtcggaa cggataaacc agtcgtaata ccaatttcgc ccggccttgc
     6421 ggcactggtg cgcaatcggg gtggctcggg ttcagccggc cacgggcggt gcggggtggc
     6481 gcagggcgaa catgccccag ccctgcatcg tcatgacgtc ctcgccatcc tggttgaaca
     6541 cgcgccagcg cgacagcacc aggccgacgt gcggcttgct cttcatcacg cgcgactcca
     6601 gcaccgtcat ctccgcggaa atcgtgtctc ccggatagac cggcttgagc cagcgaagtt
     6661 cgtcgacgcc gggcgatccc aggcccgccg agtcgttcag gtagccgtcg cacatcagcc
     6721 gcatcgtcat cgcgcaggtg tgccagccgc tggccgacag ccgcttgaac agcgaggcct
     6781 cggccgcggc gtcgtcgagg tggaagggct gcgggtcgaa ctgccgcgcg aaagcgagcg
     6841 tctcgtcgcg ggtgaccgcg cggctgccca gcgggatcac ccggccggcg gggaaatcct
     6901 cccagtagta gcgcttggcg ggcgggggtg aggtgtccat ggcgaggtcg gacctccgtt
     6961 cagtgatgcg gacgaggata gcgctcgcgc agggcgtcga gcgccgttgc cgccatgacc
     7021 ctgttcgagc ctgccgccag cgcggggctc cgggccggcg tcaccgcgac gagccggcgc
     7081 ccggcggcgc ttcccacgcc ggatggatca gcccggccag cagctcgaag cgcgtgtagg
     7141 gccgcgcgcg gccggacagc ttcagagaga tcgacaccac caggctgtgc gtgcgcgacg
     7201 gcggcgcctt ggtgggcagc accgtgagcc acgcgacgat cagcagcggc agccaccacg
     7261 gcgcgcgcac ggcgcccggg tgcgcgaggt agtcggccag cgtccagccc gacgcggcca
     7321 cgccgagcgc cgtccccgcg atcgcctcgc tccaggaatg cgcgccgagg tcgacgcgcg
     7381 aataggtgat cagcagcgcc agcgcgatgc cgaccgcgac gccgcgccgc cccgcgacga
     7441 tcgcgcccag caccggcagg atggaccacg cgtacatcga atggccggaa aagccggtga
     7501 agtcgatggc cgcgatgccg tagccgaagc ccaggaacgc gatcttgctg gcggtggtga
     7561 cggcggtggt ggccgtgatg ccggccaccc agcgcagcgc cgagcggcgg gccgggtggt
     7621 cgtgcgcgtg cgggttgcct tgcgcgaggc gcccgcgcgc acccgcggga cgcgcgaacg
     7681 cgagccacag cgcgcccgcg aggaaggccg gcaacaggat ctgggcctcg cccagccggg
     7741 tcacggcgag ccagaacagc gaatggatag cggggccgcc cgtcatgggt ccttggtcga
     7801 tgcgatcgga agcgaaagac ggcgatgcta gccgccccgc gtgtggccaa tgtgaggcga
     7861 ggctgacgct gcgccgggcg ttgctggtgg gcgtgccgtg tgacgctgtt ccgcgtgcct
     7921 gttggcgctg gcgggctgcc tcgtggagca ggtgagagtg cccggccgcg ccggagttca
     7981 accgccgcag tgcggccgcg ccagcgccag caacgtgtca tcctgcgcgc aggatgacgc
     8041 ccgatggcgt gcgctcgacg gccgtgcagg cgacggccgg acgtccgcct cagatcgccg
     8101 cagccagccg cgtgccctgg ttgatcgccc gcttggcgtc cagttccgat gcctcgtccg
     8161 cgccgccgat caggtgcacc ttgatgccgg ccgccgccag cgggccggcc agctcgcgca
     8221 gcggctcctg gcccgcgcac agcacgatgg tgtcggccgc gacgagggtg ccgtccttgc
     8281 gctcggggcc gaacgtcacc cacaggccgt cgtcggtcac gcgctcgtag ttcacgccgc
     8341 cgagcatctc cacgttcttc atcttcagcg tcgcgcgatg tacccagccc gacgtcttgc
     8401 ccaggcccgc gccgtgcttg ccggccttgc gctgcagcag cgtcacctgc cgcgccggcg
     8461 ccgatggctg cggccggtcg aggccgcccg gggtcctggc cggatcgccg acgccccatt
     8521 cgcgcttcca ctcgtccagg tgcagcgtcg ccgacggctc gccgcggtcg gcgccggtga
     8581 cgaggaactc ggccacgtcg aagccgatgc cgcccgcgcc cacgatggcc acgcgctcgc
     8641 ccacgggctt gccgtgcagc agcacgtcga tgtagctcag caccttcggg tgctcctggc
     8701 cgggaatgcg cgggtcgcgc gccttcacgc cggtggccag cacgac
//
LOCUS       DATCRO010000801         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_661752, whole genome shotgun sequence.
ACCESSION   DATCRO010000801 DATCRO010000000
VERSION     DATCRO010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661752"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VIN75_21840"
     CDS             complement(<1..379)
                     /locus_tag="VIN75_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976872.1"
                     /translation="MAKPPRSDRWGPSTGYLVIALGLVAGVLAAWWPATSAPPPATAA
                     APFASDASSSMQVALAPTASGPARATPAHDAPAPASAPARIPRVRPPGTDQSPDITDY
                     INDGEKPTIKQVIDRLHAAGVHTG"
     gene            complement(402..1511)
                     /locus_tag="VIN75_21845"
     CDS             complement(402..1511)
                     /locus_tag="VIN75_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8976873.1"
                     /translation="MSTRVPSLPARIARALARLAPALVLTGLAAQAGANTLTQNVSWT
                     IDRAGTSTKYRLVAYGDSIYAGYNTSITSAAKYSAPTVDAEYLSALWNADIESVRRAK
                     SGAVASDVYNNKIVAEKSYMQASSTRVVTFEMCGNDGLQARSAFKSQTGTCDYSGMDT
                     AVNTCKQYVAAAMDYINANAYAGVKVKVISNLHYPGYAADDTQSSCKDASTGATVNLQ
                     TTFLPRLARMNYWMCEYAREKGFTCADNFAQYMGADYDSNGDGVVDSVALQFQPGESE
                     SSYVTRVSSTLRSTLRDANTHFVSSGTSYDYIQSDDTHPTYTGGTVTAGLFGGTTGSG
                     APRYTSFTNGKSPIWNQYGHERMGWAVSTSNTAAP"
     gene            1908..>2874
                     /locus_tag="VIN75_21850"
     CDS             1908..>2874
                     /locus_tag="VIN75_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY8976874.1"
                     /translation="MREHPLSLPALAVAAVLAAAPLSSQATPVSVTNLVTDNQAANPA
                     KITDPGLVNGWGISYSPTSPFWVSSNGGGTSTLYRVDPVTQATTKLGLTVTIPGAGNP
                     TGQVFNPGGAGQFNGDAFLFVSEDGTVSGWRGALGSNAEALVSVGGIYKGAALATIGG
                     NSYLYGADFGRGSIDVFKGNAGAANLTGSFTDPNLPSGYVPFNVQNLGGSLYVTYARR
                     EGNDTDETAGAGFGYVDRYDLQGNLLARVASGGVLDAPWGLAIAPSSFGALAGALLVG
                     NFGDGRISAYDLATDTFVGQLKGVGGAPLAIDGLWGLSVGNDGGAG"
BASE COUNT          399 a          966 c         1064 g          445 t
ORIGIN      
        1 ggccggtatg gacgcccgcg gcatgcaggc ggtcgatgac ctgcttgatc gtcggcttct
       61 cgccgtcgtt gatgtagtcg gtgatgtcgg gcgactggtc ggtgccgggc gggcgcacgc
      121 gcgggatgcg ggcgggagcg ctcgcgggcg cgggcgcgtc atgcgcgggc gtcgcgcgtg
      181 cggggccgga cgcggtcggc gcgagcgcga cctgcatcga cgaggacgcg tccgaggcga
      241 acggcgcggc ggcggtggcc ggcggcggcg cgctcgtcgc cggccaccac gccgccagga
      301 cgccggccac caggccgagc gcgatcacca ggtacccggt cgacgggccc cagcgatcgc
      361 tgcgcggtgg cttggccatg cggcccgacc cctccggcgc gtcagggcgc ggcggtgttg
      421 gacgtcgaga ccgcccagcc catgcgctcg tggccgtact ggttccagat cgggctcttg
      481 ccgttcgtga acgaggtgta gcgcggcgcg cccgagccgg tggtgccgcc gaacaggccc
      541 gcggtgacgg tgccgcccgt gtaggtcggg tgcgtgtcgt cggactggat gtagtcgtag
      601 ctggtgcctg acgagacgaa gtgcgtgttg gcgtcgcgca gcgtcgagcg caacgtcgac
      661 gagacgcgcg tcacgtacga cgattcgctc tcgccgggct ggaactgcag cgccaccgag
      721 tcgacgacgc cgtcgccgtt gctgtcgtag tcggcgccca tgtactgcgc gaagttgtcg
      781 gcgcaggtga agcctttctc gcgcgcgtac tcgcacatcc agtagttcat cctggccagg
      841 cgcggcagga acgtggtctg caggttgacc gtggcgccgg tgctcgcgtc cttgcacgag
      901 ctctgcgtgt cgtccgccgc gtagcccgga tagtgcaggt tggagatcac cttgaccttg
      961 acgccggcgt acgcgttggc gttgatgtag tccatcgcgg cggccacgta ctgcttgcag
     1021 gtgttgaccg cggtgtccat gcccgagtag tcgcacgtgc cggtctggct cttgaacgcc
     1081 gagcgggcct gcaggccgtc gttgccgcac atctcgaacg tgacgacgcg cgtcgagctg
     1141 gcctgcatgt aggacttctc ggccacgatc ttgttgttgt agacgtcgga ggcgacggcg
     1201 ccggacttgg cgcggcgcac gctctcgatg tcggcattcc acagcgcgga caggtactcc
     1261 gcgtcgacgg tcggcgccga gtacttggcg gcgctggtga tggacgtgtt gtagccggca
     1321 tagatcgaat cgccgtaggc caccagccgg tacttggtgg acgtgccggc gcggtcgatc
     1381 gtccacgaga cgttctgcgt cagcgtgttg gcgccggcct gcgcggccag gccggtcagc
     1441 acgagcgcgg gcgcaagtcg cgcgagtgcg cgggcgatgc gtgcgggcag ggaggggact
     1501 cgggtgctca tctggttctc ctggatttgt gcttggacgg gggaacagaa cgcgcgagtc
     1561 cgtgggcccg gagtcggcgt gacgcgagtc tggacacgcg acctcgccag gggatgcgga
     1621 ggaacccggt ccgcgcgacg ctgccggaac gcgcggacaa gcacttggct cgcggagaca
     1681 gccgacttgc aaataggcac caattcacgc cgggccgata cccaaggggg catgggatcg
     1741 acagcatggc gtcagccgtc cgacagccgc cgcgactgtt acgcgccgcc gagtgaacgg
     1801 ttcggcgtcc cgaggtcatg cacggatcgg cgggccgctg cgtccgcctc gcgcgtcgcg
     1861 tccagccggg cgcggcgcag gcgcttccca aaccagagga gatcccgatg agagagcatc
     1921 cgctatccct gccggccctc gcggtcgccg ccgtgctggc cgcggcgccg ctgtcgagcc
     1981 aggcgacgcc ggtgtcggtc accaacctgg tcaccgacaa ccaggcggcc aatccggcga
     2041 agatcaccga ccccggcctc gtcaacggct ggggcatctc gtattcgccg accagcccgt
     2101 tctgggtgtc gtccaacggc gggggcacgt ccacgctgta ccgcgtcgac ccggtcacgc
     2161 aggccaccac caagctcggc ctgacggtga ccatccccgg cgcgggcaac ccgaccggcc
     2221 aggtgttcaa ccccggcggc gcaggccagt tcaacggcga cgcattcctg ttcgtcagcg
     2281 aggacggcac cgtctcgggc tggcgcggcg cgctgggcag caacgccgag gcgctggtct
     2341 cggtcggcgg catctacaag ggcgcggcgc tggccaccat cggcggcaac agctacctgt
     2401 acggcgcgga cttcggccgc ggctcgatcg acgtcttcaa gggcaacgcc ggcgccgcca
     2461 acctgaccgg ctcgttcacc gacccgaacc tgccctcggg ctacgtgccg ttcaacgtgc
     2521 agaacctggg cggctcgctg tacgtcacct acgccaggcg cgagggcaac gacaccgacg
     2581 agaccgcggg cgccggcttc ggctacgtcg atcgctacga cctgcagggc aacctgctgg
     2641 cccgcgtggc cagcggcggc gtgctcgacg cgccgtgggg cctggcgatc gcgccgtcgt
     2701 cgttcggcgc gctggccggc gcgctgctgg tgggcaactt cggcgacggc cgcatcagcg
     2761 cgtacgacct cgccacggac accttcgtcg gccagctgaa gggcgttggc ggtgctcccc
     2821 tggcgatcga cggcctgtgg ggactgagcg tcggcaatga cggcggcgcg ggca
//
LOCUS       DATCRO010000802         4923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_583186, whole genome shotgun sequence.
ACCESSION   DATCRO010000802 DATCRO010000000
VERSION     DATCRO010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583186"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="cysD"
                     /locus_tag="VIN75_21855"
     CDS             <1..782
                     /gene="cysD"
                     /locus_tag="VIN75_21855"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8976875.1"
                     /translation="LHLAKKAFYPSPPPFPLLHVDTTWKFRAMYEMRERMARESGMEL
                     LVHQNPEAAELGINPFTHGSQIHTDMWKTQGLKQALDKYNFDAAFGGARRDEEKSRAK
                     ERIFSFRSAQHRWDPKNQRPELWRLYNGRKMKGESIRVFPISNWTELDIWQYIYLENI
                     PIVPLYFAAERPVVNRDGTLIMVDDDRMPLKAGEVPMMKSVRFRTLGCYPLSGAVESE
                     AATLKDVIQEMLLTKTSERQGRMIDHDSAASMEKKKQEGYF"
     gene            835..2739
                     /gene="cysN"
                     /locus_tag="VIN75_21860"
     CDS             835..2739
                     /gene="cysN"
                     /locus_tag="VIN75_21860"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY8976876.1"
                     /translation="MAHQSDLIATDIEKYLKQHERKSLLRFITCGSVDDGKSTVIGRL
                     LYESKMLFEDQLAAIENDSKKWGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTD
                     RRKFIVADTPGHEQYTRNMITGASTADVAVILVDARKGVLTQTRRHSYLARLIGIRKV
                     VLAINKMDLVDYSQKVFDQIDEEYRAFARQIGLADITSIPLSGLKGDNMLVASEKTPW
                     YHGPTLMGFLETCEVDDTRLQKEAFRMPVQWVNRPNLDFRGFAGIVASGTVKPGDRVI
                     AQPSGKESTVARIVAMGGDLPQAVAGQSITLTLADEIDISRGDVLSMAEAPAEVADQF
                     EASLVWMTDEPMLPGRPYLMKIGTQTVTASITEPKYKVNVNTMEHLAAKQLGVNEIGV
                     VNLALDRQIAFDAYKNNRDTGGFILINRMTNNTVGAGMINFALRRAHNLRPQHVDVDK
                     ARRSELKGQRPAVLWFTGLSGAGKSTIANLVEKKLAAMGRHTYLLDGDNVRHGLNKDL
                     GFTEADRVENIRRVAEVARLMVDAGLIVMTAFISPFRSERAMARGQMADGEFIEIHVA
                     TPLAVAEERDVKGLYKKARRGEIANFTGISSPYEAPETPEIVVDTHTQTADEAAEAII
                     AKLKSLGLIE"
     gene            complement(2743..3912)
                     /locus_tag="VIN75_21865"
     CDS             complement(2743..3912)
                     /locus_tag="VIN75_21865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8976877.1"
                     /translation="MSAPSGTDLYAEFRDTAYFPSLNGIRAICALMVLKIHTDWSVPG
                     APRILDCGFLGVDMFFAISGFLIVTLLLREREATGRIDLKQFYVRRTLRIFPIYYLLV
                     GLLLLLAVATYGHSTRTWDDYKWSFPVFLLYLQDAVPIFMGVLFHTWSLSMEEQFYLV
                     WPSVERFLRRAWIVPVLALLIAVNEMFNFRVFDNWIVDVYGPQGLHRPLFLITFSPIL
                     LGVLAAHVMHDPRSGRIAAAALGNRWMPPLLMVLATLVVQYAAELRGPGQSVVLRGLP
                     YTAVHVLFCLALVAMVVNPRGIFSRTLQSRPLAYLGSISYGIYLYHTMIIWLIDRVCT
                     PRHIHLSPFALFVLVSALSICVAALSFRYFETPIMRSRHRKRAAAVVATEAPAAT"
     gene            complement(3909..>4923)
                     /locus_tag="VIN75_21870"
     CDS             complement(3909..>4923)
                     /locus_tag="VIN75_21870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8976878.1"
                     /translation="ILAAMTVGIVIVSYKSAALTVDCLRSVATERAGPGPRIRCVVID
                     NASGDEPVVAAAIADNGWGDWARVVVAPRNGGFAYGNNLGLKELERDGRVDFVHLLNP
                     DTVLRPGAVAALADWLHATPRAGIAGGVFENGDGSEWAIAFRFPSLLGEIEQGVRLGL
                     VSRMLSRACVPMHMGASAAQVDWVSGASTMVKREVFDRIGGLDETFFLYFEETEFCHR
                     AKRAGYEVWYVPASRVVHIAGQSTKVTERNAGPKRLPSYWFDSRRLYFTLTHGPAYAA
                     LADALAIVAGWAGLAKSALLGRLRGHVPHFLRDLFRHSALSGNAGATRAAPPRFDQAR
                     GTR"
BASE COUNT          909 a         1697 c         1656 g          661 t
ORIGIN      
        1 tgctgcacct ggccaagaag gcgttctacc cctcgccccc gcccttcccg ctgctgcatg
       61 tggacaccac gtggaagttc cgcgccatgt acgagatgcg ggagcgcatg gccagggaat
      121 cgggcatgga gctgctggtg caccagaacc ccgaggcggc ggagctgggc atcaacccgt
      181 tcacgcacgg ctcgcagatc cacaccgaca tgtggaagac gcagggcctg aagcaggcgc
      241 tggacaagta caacttcgac gcggccttcg gcggcgcgcg ccgcgacgag gagaagtcgc
      301 gcgccaagga gcgcatcttc tcgttccgct ccgcgcagca tcgctgggat ccgaagaacc
      361 agcgccccga gctctggcgc ctgtacaacg gccgcaagat gaagggcgag tcgatccgcg
      421 tgttcccgat ctcgaactgg accgagctgg acatctggca gtacatctac ctggagaaca
      481 tccccatcgt gccgttgtac ttcgcggccg agcgcccggt ggtcaaccgc gacggcacgc
      541 tgatcatggt cgacgacgac cgcatgccgc tcaaggccgg cgaggtgccg atgatgaaga
      601 gcgtgcgctt ccgcaccctc ggctgctatc cgctgtcggg cgccgtcgag tccgaggccg
      661 cgacgctcaa ggacgtgatc caggagatgc tgctgacgaa gacgtcggag cgccaggggc
      721 gcatgatcga ccacgactcc gccgcctcga tggagaagaa gaagcaggaa ggctatttct
      781 gaggccccgc gccgccagga agaccctctt caacgcacac acaggacacc catcatggcc
      841 caccaatcgg acctgatcgc caccgacatc gagaagtacc tcaagcagca cgagagaaag
      901 agcctgctgc gcttcatcac ctgcggctcc gtggacgacg gcaagtccac cgtcatcggc
      961 cggctgctgt acgagtcgaa gatgctgttc gaggaccagc tcgccgccat cgagaacgac
     1021 tccaagaagt ggggcacgca gggcggcgac atcgacttcg cgctgctggt cgacggcctg
     1081 gccgccgagc gcgagcaggg catcacgatc gacgtcgctt accggttctt ctccaccgac
     1141 cgccgcaagt tcatcgtcgc cgacaccccc ggccacgagc agtacacgcg caacatgatc
     1201 acgggcgcgt ccaccgccga cgtcgccgtc atcctggtcg acgcgcgcaa gggcgtgctg
     1261 acgcagacgc gccgccacag ctatctcgcg cgcctgatcg gcatccgcaa ggtggtgctg
     1321 gccatcaaca agatggacct cgtggactac tcgcagaagg tgttcgacca gatcgacgag
     1381 gagtaccgcg cgttcgcccg ccagatcggc ctggccgaca tcacgtcgat cccgctgtcc
     1441 ggtctcaagg gcgacaacat gctggtggcc agcgagaaga cgccctggta ccacgggccg
     1501 acgctgatgg gctttctcga gacctgcgag gtcgacgaca cgcgcctgca gaaggaagcc
     1561 ttccgcatgc cggtgcaatg ggtcaaccgc cccaacctcg acttccgcgg cttcgccggc
     1621 atcgtcgcca gcggcaccgt caagccgggc gaccgcgtga tcgcgcagcc ttcgggcaag
     1681 gagagcaccg tcgcgcgcat cgtcgccatg ggcggcgacc tgccgcaggc cgtcgcgggc
     1741 cagtcgatca cgctgacgct ggccgacgag atcgacatct cgcgcggcga cgtgctgtcg
     1801 atggccgagg cccccgccga ggtggccgac cagttcgagg cctcgctggt gtggatgacc
     1861 gacgagccga tgctgccggg ccgcccctac ctgatgaaga tcggcacgca gacggtgacg
     1921 gcctccatca ccgagcccaa gtacaaggtc aacgtcaaca cgatggagca cctggccgcc
     1981 aagcagctgg gcgtcaacga gatcggcgtc gtcaacctcg cgctcgatcg ccagatcgcg
     2041 ttcgacgcgt acaagaacaa ccgcgacacc ggcggcttca tcctgatcaa ccgcatgacc
     2101 aacaacacgg tcggcgccgg gatgatcaac ttcgcgctgc gccgcgcgca caacctgcgc
     2161 ccgcagcacg tggacgtcga caaagcgcgg cgctccgagc tgaagggcca gcggccggcg
     2221 gtgctctggt tcacgggcct gtccggcgcg ggcaagtcca ccatcgccaa cctcgtcgag
     2281 aagaagctcg ccgcgatggg ccgccacacc tacctgctgg acggcgacaa cgtgcgccac
     2341 ggcttgaaca aggacctcgg cttcaccgag gccgaccgcg tggagaacat ccgccgggtg
     2401 gccgaggtgg cgcgcctgat ggtcgacgcc ggcctgatcg tgatgaccgc cttcatctcg
     2461 ccgttccgct cggaacgcgc gatggcgcgc ggccagatgg ccgacggcga gttcatcgag
     2521 atccatgtcg ccacgccgct ggccgtggcc gaggagcgcg acgtcaaggg cctgtacaag
     2581 aaggcgcgcc gcggcgagat cgccaacttc accggcatca gctcgccgta cgaggcgccg
     2641 gagacgccgg agatcgtcgt cgacacgcac acgcagacgg ccgatgaggc cgccgaggcg
     2701 atcatcgcga agctgaagtc gctcgggctg atcgaatgac gttcaggtcg ccgcaggcgc
     2761 ctcggtcgcg acgacggcgg ccgcgcgctt gcggtggcgc gatcgcatga tcggcgtctc
     2821 gaagtagcgg aagctcaagg cagccacgca gatcgagagc gcgctcacca gcacgaacag
     2881 cgcgaacggg ctcaggtgga tgtggcgcgg cgtgcagaca cggtcgatca gccagatgat
     2941 catcgtgtgg tacaggtaga tgccgtagct gatcgagccg aggtacgcga gcggccgcga
     3001 ctgcagcgtg cgcgagaaga tcccgcgcgg attgacgacc atcgccacca gcgcgaggca
     3061 gaacagcacg tgcacggccg tgtacggcag cccgcgcagc accaccgact gcccggggcc
     3121 ccgcagctcc gccgcgtact gcaccaccag cgtcgccagc accatcagca gcggcggcat
     3181 ccagcggttg cccagcgccg cggcggcgat gcgcccgctg cgcgggtcat gcatcacatg
     3241 ggcggccagc acgcccagca ggatcggcga gaacgtgatc aggaacagcg gccggtgcag
     3301 tccctgcggc ccgtacacgt cgacgatcca gttgtcgaag acacggaagt tgaacatctc
     3361 gttgaccgcg atcagcagcg cgagcaccgg cacgatccag gcgcggcgca ggaagcgctc
     3421 gacgctgggc cagacgaggt agaactgctc ctccatcgac agcgaccacg tgtggaacag
     3481 cacgcccatg aagatcggca cggcgtcctg caggtacagc aggaagaccg ggaacgacca
     3541 cttgtagtca tcccacgtcc tggtcgagtg gccgtaggtg gccacggcca gcagcagcag
     3601 caagcccacc agcaggtagt agatcgggaa gatgcgcagc gtgcgccgca cgtagaactg
     3661 cttcaggtcg atgcggcccg tggcctctcg ctcgcgcagc agcagcgtca cgatcaggaa
     3721 gccgctgatc gcgaagaaca tgtccacgcc gaggaagccg cagtcgagga tgcgcggggc
     3781 gcccggcacg ctccagtcgg tgtggatctt gagcaccatc agcgcgcaga tcgcgcggat
     3841 gccgttcagc gacgggaagt aggccgtgtc gcggaactcg gcgtacaggt cggtgcccga
     3901 gggcgcgctc atcgcgtgcc ccgtgcctgg tcgaagcgcg gcggcgccgc gcgcgtcgcg
     3961 ccggcgttgc ccgacagcgc gctgtggcgg aacaggtcgc gcaggaagtg cggcacgtgg
     4021 ccgcgcagcc gcccgagcag cgccgacttc gccaggcccg cccagccggc gacgatcgcc
     4081 agcgcgtccg caagcgcggc gtacgccggg ccgtgcgtca gcgtgaagta gagacggcgc
     4141 gagtcgaacc agtacgacgg caggcgcttc gggccggcgt tgcgctcggt caccttggtg
     4201 ctctggcccg cgatgtgcac gacgcggctg gccggcacgt accagacctc gtagccggcg
     4261 cgcttggcgc ggtggcagaa ctcggtctcc tcgaagtaca ggaagaacgt ctcgtccagg
     4321 ccgccgatgc ggtcgaacac ctcgcgcttg accatcgtcg acgcgcccga cacccagtcg
     4381 acctgcgccg cgctcgcgcc catgtgcatg ggcacgcacg cgcgcgagag catgcgcgag
     4441 acgagcccga gccgcacgcc ctgctcgatc tcgcccagca gcgacgggaa gcggaacgcg
     4501 atggcccact cgctgccgtc gccgttctcg aacacgccac cggcgatgcc tgcgcgcggg
     4561 gtcgcgtgca gccagtcggc cagcgcggcg acggcgccgg ggcgcagcac ggtgtcgggg
     4621 ttgagcaggt ggacgaagtc gacgcggccg tcgcgctcga gctccttcag gcccaggttg
     4681 ttgccgtacg cgaagccgcc gttgcgcggc gcgacgacga cgcgcgccca atcgccccag
     4741 ccgttgtccg cgatcgcggc ggccacgacg ggctcgtcgc ccgacgcgtt gtcgatgacg
     4801 acgcagcgga tgcgtggccc ggggccggcg cgttccgtcg cgacggagcg caggcagtcg
     4861 acagtcagcg ccgccgactt gtagctgacg atgacgatgc ccaccgtcat cgccgcgagg
     4921 ata
//
LOCUS       DATCRO010000803        11269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_549554, whole genome shotgun sequence.
ACCESSION   DATCRO010000803 DATCRO010000000
VERSION     DATCRO010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549554"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VIN75_21875"
     CDS             <1..745
                     /locus_tag="VIN75_21875"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8976879.1"
                     /translation="RRQQQLADAGFVSPAGVEQSARNKELADRLLRQAEADQHAEAAI
                     REQSISEMSDAVRGLQRGLQLLERTRERLLQRAPLAGTLSGLQLQVGASVRPGDRLGR
                     IDDPAGGVQLVADVDEYWLPRLQPGQAVASDSGDLVLAQALPQVQAGKVRVLLRWPAG
                     RSPASLRAGQAVDVRLQLSPPTRALLLPEGPGVQSRVYVRQGGTLHRRDVRLGRRASG
                     LVEILGGLLAGDEVLISQPPADTDTLALP"
     gene            742..1428
                     /locus_tag="VIN75_21880"
     CDS             742..1428
                     /locus_tag="VIN75_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8976880.1"
                     /translation="MIDLHQLTKRHRAGDIETTALDAIDLHIEAGEYVAITGPSGCGK
                     STLLGLLGLLDRPTSGRYRLQGEDVAGKSPRELAALRRGRIGFVFQSFNLVDEMTVHD
                     NVQLALRYGGQPEKAQRARVAEVLERLGLAHRAKHHPSQLSGGQQQRVAIARAIAGRP
                     ALLLADEPTGNLDSAHGQEVMRVLRELNGEGTTLVMVTHSPEHAALASRTVRLLDGRV
                     LVDAHAEQLS"
     gene            1425..3863
                     /locus_tag="VIN75_21885"
     CDS             1425..3863
                     /locus_tag="VIN75_21885"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8976881.1"
                     /translation="MKAQLLDALRDVRSRGRATAVAIAGLTIGLAACLLVALFALALA
                     APDPDVADPERTIMLDFKGNPPGEPSPWFGAVPVAFGPLLKARRAPLDLVSRAGDNGI
                     ELTEDGRSNPALVLAVDPDIVDVLGLHSLAGDLRATLRGHDTIAITADLLRKLWGSLP
                     PAQAIGRRVQGHGRWYTVTAVLPDTDPRSPLYGASPLVGGAMVMCNFDSPANEMSTND
                     REAIFLMTGHVFARLRPGTSIDQVGGWMRDAFVHSPQFRQLPPAWRTGREAAFFRGIT
                     ISDIPFDGPNRVARWEQLAAVGAASVLLLVLAALNAMSLQAAHMLQRQRETALRQALG
                     AAGPHLVQLWAIEALVPLLAGAAGALLVAWWIAPTIANWLQLPAGLPLADPIPAKAWL
                     GLGGVVLALVPLTIALPARAALRRVPATALQGRTASEGPWGRRLRQSLLGLQLGGALL
                     LLSVSGVLALQQRHVLHADRGFDTHDRLVLMMETDPDHVPRLDAFEAALSHDPAVRHW
                     SYSYSPPAADWGGPQQRELDTSADGHTVDLRMSSVGASFFDTYGMTVLAGTPRYAEGE
                     HHVVIDAKAARLLGFASPQAAVGAIVQGGGAYLQQGSETRRIVAVVKDVNFASAQSPA
                     MPQAFWLMDQPQWVVTATGPDPKALWNALDGAWKAHGLKVPYELKWADDQRAEVYRQE
                     GQLATTVAVVALLAVLVAMIGAYAMVADTLRRRRTEFVLHRLHGAGDAAIARQVAGEF
                     AVPLLGAALAALPAAALIGARWLGGFHDRVGWLPGLVAPLAAALAATLLVTALACLRH
                     VRQALALQPIEALA"
     gene            complement(3867..4079)
                     /locus_tag="VIN75_21890"
     CDS             complement(3867..4079)
                     /locus_tag="VIN75_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976882.1"
                     /translation="MFDPSTHPRPPAAVAHGAGRVLTVAELAVVLEELDRVVAELQES
                     LAEIALPLDDASRCAARDAIAAARRH"
     gene            complement(4446..4760)
                     /locus_tag="VIN75_21895"
     CDS             complement(4446..4760)
                     /locus_tag="VIN75_21895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976883.1"
                     /translation="MTDPTIRDNAAAHRFEKIVDRHAAVATYSLAGDVITFEHTVVPE
                     ALRGRGIARELVEAALASVRERGLKVVPRCEVFDAYMRKHPETHDLLADPHQFDEGAR
                     AA"
     gene            4990..6687
                     /locus_tag="VIN75_21900"
     CDS             4990..6687
                     /locus_tag="VIN75_21900"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEY8976884.1"
                     /translation="MELPVRMIGSRAVATVGINGTDVPLVVDSGAFFSMLTPAAADQL
                     NLRRHSLPRGMTIQGLAGNAEAQATTVDRLQLFKGEIPNVDFIVGGNEDVPGTMGLLG
                     RNILSAVDTEYDLAHGMIRLVIPSDECEESVMAYWAAGMQVAELRLLRAFNERQPSIR
                     AKVKINDATVTALFDTGAMTLLSLRAAHWAGLKDGDLSPNGRHYGIGTDSVNSWTAKI
                     AKVDFGGEAIMNNTLVVDDYDMADNDMLVGIDFFLSHRIYVSKRQSKMYFTYAGGPVF
                     ARNKGELADTAAADSAAASGAATMTADALARRGEASLSRGNVASALADLDQACAMEPV
                     NASHRWARARAHLAMKDVPAARADLDKAIELAPRLAEARIVRAGLREAAHEREAALDD
                     LAALDTALPAPSDIRRGMAMLYGEMHMPAQAIAQWNQWLPAHVHDIGRPAALNARCWS
                     RVELGVELDKALADCDEAIDIDGKNASYLDSRAWTYLRMGKLKKALADFDRAIEINPK
                     GAWSLYGRGQVQLGLGQAERARADLAAARTERPGIDADAKRDGLPQAPIAPPSPASP"
     gene            complement(6701..7378)
                     /locus_tag="VIN75_21905"
     CDS             complement(6701..7378)
                     /locus_tag="VIN75_21905"
                     /inference="COORDINATES: protein motif:HMM:NF022885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HEY8976885.1"
                     /translation="MAETTAADGVAPEPAPDAQAPVRDAQARDPVRLRLAEALARRAN
                     AHAGEARRRIDERVAAIVQSLSELPERAPPARPVAPAAGLGALAALVAHAARRRGAAP
                     GAVPGARPVAAAPGAAAPAVTGAAADEKTVQFFKRTWSRLSADQRLAQSRASLPENAG
                     PLNSQHLVHRSLVLMRELSPEYLEHFVGYIDALQWLEQANEAATQELTNARAAQGRPR
                     SMAARRS"
     gene            complement(7371..8057)
                     /locus_tag="VIN75_21910"
     CDS             complement(7371..8057)
                     /locus_tag="VIN75_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8976886.1"
                     /translation="MDDIDVDGAADASAPVWAVFGDLMSGLLGAFVLILLFALASQLE
                     LVTRLQDEIRQHQLEEQRRAALEKALSVPLAAGRVTIDHGRIDINGSVLFAFNSAELQ
                     PEGLALLRQLAGPVTTYLKARDEILMVSGFTDDRQVRGGNHQFADNWELSAQRSLTVT
                     RALIEAGVPSSDIFAAAFGAEQPVADNAQADGRARNRRVEMAPTPRTARARPVAGAAS
                     VPVAASAAHG"
     gene            complement(8184..10391)
                     /locus_tag="VIN75_21915"
     CDS             complement(8184..10391)
                     /locus_tag="VIN75_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF802 domain-containing protein"
                     /protein_id="HEY8976887.1"
                     /translation="MNQFLRHAAAVTGLAVIAWVGAGYLLANPLALVLTLLIGAFYAM
                     GVLELRRFRQATSALATAVDALAATPASLAPWLEQLPAGLRNAVRRRVEGEPVGLPGP
                     ALAPMLAGLLVLLGMLGTFLGMILTLRGTGAALETATDLVAVRNSLIAPVKGLGLAFG
                     TSVAGVAASAALGLLSALARRERVAVAQRLDARIATTLRPFSVSHQREEQLALLQRQA
                     ELMPALVDGLKAMMATMERQAEASQARLLASQERFQGQAEIAYVALAQSVDRTLQASL
                     TEGARIAGETIRPVAEATLAGIARETATLHATLAGTMREHVAQLSSSFAASTANSAES
                     WQALLARHEQAAQSLAADVEASHARFAQTFEQRSTALLDQVAQRHAGWQGEMATTLAT
                     LARETAATQQAIAGATATQLEGAGARLDAAVARVTQAWADAVARQQDAGAALAASTER
                     SLSTAAATFEQHSASLLQTVQQAHAELQATSAARDQQRVDAMASSLEAMAASLQAQWQ
                     QAGAQSLEQQQRVIEAMERTARDITAQAETQARSTIAEIGRLVAAASEAPRAAAEVIS
                     ELRQKLADSMARDNTMLDERARILETLGTLLDGVNRAAGEQRGAIDALVTSSAEVLER
                     VGERFQAQVQAEAGKLVDVAAQVTGSAVEVASLGEAFGVAVQRFTESGDRLGAHLERI
                     DVALGAQMERSDEQLAYYVAQAREVIDLSILSQKQIIEELQRLAGRRAEEAAA"
     gene            complement(10388..11128)
                     /locus_tag="VIN75_21920"
     CDS             complement(10388..11128)
                     /locus_tag="VIN75_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 family protein"
                     /protein_id="HEY8976888.1"
                     /translation="MSGSLAREARRIPLTGSTFIRALAALDDASVPSAPDAFAQRLAR
                     WFDWTHAFSLSAALGDASARADAPTFGGQGALAADEREFARVKASLAKAIADAPTGGA
                     AAARRIVRPLKDAVAPAEMPVDFATLRQRYTKCQLAMETQIVPLRRRLRSTLADRSSR
                     AAKLAELDTVMEQVVGAQERALLATVAGRLEARFDRLRGPDADAPAGPWLERFGQEMR
                     GLLLAELDLRLQPCEGLLQACRQSLHRT"
BASE COUNT         1506 a         4105 c         4157 g         1501 t
ORIGIN      
        1 gcggcgccag cagcagctgg ctgacgccgg cttcgtgtcg ccggccgggg tcgaacagag
       61 cgcgcgcaac aaggaactcg ccgaccgcct gctgcgccag gccgaggccg accagcacgc
      121 cgaggccgcg atccgggaac agtcgatcag cgagatgagc gacgccgtgc gcggcctgca
      181 gcgcgggctg cagttgctcg aacgcacgcg cgagcgcctg ctgcagcgcg cgccgctggc
      241 cggcacgctc agcggcttgc agctgcaggt cggcgccagc gttcggcctg gggatcgcct
      301 cggccgcatc gacgatccgg ccggcggcgt gcagctcgtg gccgatgtcg acgagtactg
      361 gctgccgcgg ctgcagcctg gccaggccgt cgccagcgac tccggcgacc tcgtgctggc
      421 gcaggcgctg ccgcaggtgc aggccggcaa ggtgcgcgtg ctgctgcgct ggcccgcggg
      481 ccgatcgccg gccagcctgc gcgccggcca ggcggtcgac gtccgcctcc agctgagccc
      541 gccgacccgg gcgctgctgc tgcccgaggg acccggtgtc caatcgcggg tgtacgtgcg
      601 ccagggcggc acgctgcacc ggcgcgacgt caggctcggc cggcgcgcgt ccggcctcgt
      661 cgagatcctc ggcggcctgt tggccggcga cgaggtgctc atctcccaac cgcccgccga
      721 caccgacacc ctcgccctgc catgatcgat cttcaccagc tgaccaagcg ccatcgcgcc
      781 ggcgacatcg agaccaccgc gctcgacgcc atcgacctgc acatcgaggc cggcgaatac
      841 gtcgccatca cggggccgtc cggctgcggc aagtccacgc tgctgggcct gctcggactg
      901 ctcgatcgac cgacctccgg acgctaccga ctgcagggcg aggacgtggc cggcaagagc
      961 ccgcgcgagc tcgcggcgct gcgacgcggt cgcatcggct tcgtcttcca gagcttcaac
     1021 ctggtcgatg agatgacggt gcacgacaac gtgcagctcg cgctgcgcta cggcggccag
     1081 cccgagaagg cgcagcgcgc gcgcgtcgcc gaggtgctgg aacgcctcgg cctcgcgcat
     1141 cgtgcgaagc atcatcccag ccagctgagc ggcggccagc agcagcgcgt cgcgatcgcg
     1201 cgcgccatcg cgggccggcc cgcgctgctg ctcgccgacg agcccaccgg caacctcgac
     1261 agcgcgcacg gccaggaggt gatgcgcgtg ctgcgcgagc tcaacggcga gggcacgacg
     1321 ctggtgatgg tgacgcacag ccccgagcac gcggcgctcg catcgcgcac ggtgcggctg
     1381 ctcgatggcc gcgtgctcgt cgacgcgcac gcggagcagc tgtcgtgaag gcccagctgc
     1441 tggacgcgct gcgcgacgtg cgcagccgcg gccgcgccac cgcggtcgcc atcgccggcc
     1501 tgacgatcgg cctggccgcc tgcctgctgg tggcgctgtt cgcgctcgcg ctggccgcgc
     1561 ccgatcccga cgtggccgac cccgagcgca ccatcatgct ggatttcaag ggcaatccgc
     1621 ccggcgagcc gagcccgtgg ttcggtgcag tgccggtggc cttcgggccc ctgctcaagg
     1681 cacgccgcgc gccactggac ctggtcagcc gcgccggcga caacggcatc gagctcaccg
     1741 aggacggccg ctcgaatccc gcgctggtgc tcgcggtcga tccggacatc gtcgacgtgc
     1801 tgggcctgca cagcctggcc ggcgacctgc gcgccacgct gcggggccac gacaccatcg
     1861 ccatcaccgc cgacctgctg cgcaagctct ggggatccct gccgcccgcg caggcgatcg
     1921 gccggcgcgt gcagggccac ggccgctggt acacggtcac cgcggtgctg cccgacaccg
     1981 atccgcgcag cccgctgtat ggtgcgagcc cgctggtggg cggcgcgatg gtgatgtgca
     2041 acttcgactc gccagccaac gagatgagca cgaacgaccg cgaggccatc ttcctgatga
     2101 ccggccacgt gttcgcgcgc ctgcggccgg gcacctcgat cgaccaggtc ggcggctgga
     2161 tgcgcgacgc cttcgtccac agcccgcagt tccggcagct gccgccggcg tggcgcacgg
     2221 gccgcgaggc ggccttcttc cgcggcatca cgatcagcga catcccgttc gatggcccga
     2281 atcgcgtggc gcgctgggaa cagctggccg ccgtcggcgc cgcgagcgtg ctgctgctgg
     2341 tgctggccgc gctcaacgcg atgagcctgc aggcggcgca catgctgcag cgccagcgcg
     2401 agaccgccct gcgccaggcc ctgggcgcgg ccggcccgca cctggtgcag ctgtgggcga
     2461 tcgaggcgct cgtgccgctg ctggccggcg cggcaggcgc cctgctggtc gcgtggtgga
     2521 tcgcgccgac catcgccaac tggctgcagc tgccggccgg cctgccgctc gccgacccga
     2581 tccccgcgaa ggcctggctc gggctgggcg gggtcgtgct cgcgctcgtc ccgctcacga
     2641 tcgcgctgcc cgcgcgcgcc gcgctgcgac gcgtgccggc caccgcgctg caggggcgca
     2701 cggccagcga agggccgtgg ggccgccggc tgcggcagag cctgctgggg ctgcagctgg
     2761 gtggagcgct gctgctgctg tcggtgtcgg gcgtgctggc gctgcagcag cgacacgtgc
     2821 tgcacgccga ccgcggcttc gacacgcacg accgcctcgt gctgatgatg gagaccgatc
     2881 ccgaccacgt gccgcgcctg gacgcgttcg aggccgcgct gtcgcacgac cccgccgtcc
     2941 gccactggtc gtacagctac agcccgcctg ccgccgactg gggcgggccg cagcagcggg
     3001 agctcgacac cagcgccgac ggccacaccg tcgacctgcg catgtcctcg gtgggcgcga
     3061 gcttcttcga cacctacggc atgaccgtgc tcgccggcac gccgcgctac gcggagggcg
     3121 agcaccatgt cgtgatcgac gccaaggccg cgcgcctgct cggcttcgcg tcgccgcagg
     3181 cggcggtcgg cgcgatcgtc cagggcggcg gcgcctacct gcagcagggc agcgagacgc
     3241 gccgcatcgt ggcggtggtc aaggacgtga acttcgcgag cgcgcaaagc cctgcgatgc
     3301 cccaggcctt ctggctgatg gaccagcccc agtgggtggt gacggccacg ggccccgatc
     3361 cgaaggccct gtggaacgcg ctcgacggcg cctggaaggc acacggcctg aaggtgccct
     3421 acgaactgaa atgggccgac gaccagcgcg ccgaggttta ccgccaggaa ggccagctgg
     3481 cgacgaccgt cgccgtcgtc gccctgctcg cggtgctcgt cgccatgatc ggcgcctacg
     3541 cgatggtggc cgacacgctg cggcgccgtc gcaccgagtt cgtcctgcac cgcctgcacg
     3601 gcgccggcga cgcggcgatc gcgcgccagg tggccggcga gttcgctgtg ccgctgctgg
     3661 gcgcggcgct ggcggcgctg ccggcggcgg cgttgatcgg cgcgcgctgg ctcggcggct
     3721 tccacgaccg cgtcggctgg ctgccgggcc tggtcgcgcc actggcggcc gcgctggccg
     3781 cgaccctgct cgtcaccgcg ctggcctgcc tgcgccatgt gcgccaggcg ctggcgctgc
     3841 agccgatcga ggcgctggcc tgacgttcag tgccggcgcg ccgcggcgat ggcatcgcgc
     3901 gccgcacagc gcgaggcgtc gtcgagcggc agcgcgatct cggccagcga ttcctgcagc
     3961 tcggcgacga cgcgatcgag ctcctcgagc acgacggcaa gctcggcgac ggtaagcacg
     4021 cggccggcgc cgtgcgcgac ggcggcgggc ggccggggat gggtcgaggg atcgaacatg
     4081 cggggctcct tcgcgccggg cggcgccttg gcgcgtttca ggcattccac gcgcccgcga
     4141 gagggcgtgc atgccaaggc atgcgggttg ccgatggtga ggttggactg ccgctctcgt
     4201 tcgcggacac gcgtcgtgca cgcaggcatg agccaaggcc ggccaacgcg tcgacgggtt
     4261 cgacgaggcc tggtctgccg gagtggatcc tgcgactgcg caggatgaca cctggtgtgc
     4321 gcgacgcggc gacgcatggt gtcgtccttc gcgaacacgc catcaccgcg gcaagtaccc
     4381 aaccacgccg tcatcctgcg cggagccgag cgcagtcgca ggatccacct cggtcgagga
     4441 ggccgctacg ccgcgcgagc gccttcgtcg aactggtgcg ggtccgcgag caggtcgtgc
     4501 gtctcgggat gcttgcgcat gtaggcatcg aacacctcgc atcgcggcac caccttgagt
     4561 ccgcgctcgc gcaccgaggc cagcgcggcc tccaccagtt cgcgcgcgat gccgcgccca
     4621 cgcagcgcct cgggcacgac ggtgtgctcg aaggtgatca cgtcgccggc cagcgagtag
     4681 gtcgcgaccg cggcgtggcg gtcgacgatc ttctcgaagc ggtgggcggc ggcgttgtcg
     4741 cggatcgtgg ggtcggtcat ggcaggggca ttccggtagc gtcgatggcg ccattttccc
     4801 gtggcctcat ggcggcgcct gcgacgtggc gtaaagtcga gcaaaaaaag gggaggcctc
     4861 ttgaacgcag atcgattcga tcgaaggacg gcaccgcgcc gcgggtgccg gcgctggctg
     4921 gcgggttcgc tggccgccct gaccctgctg tgtcccgcca cgggacgggc cgcatgcagt
     4981 gtgaagtaca tggaactgcc cgtgcgcatg atcggctcgc gcgcagtggc caccgtgggc
     5041 atcaatggca ccgacgtccc gctggtcgtc gacagcggcg cgttcttcag catgctcacc
     5101 ccggcagccg ccgaccagct gaacctgcgc cgacattcgc tgccccgcgg catgaccatc
     5161 cagggtctgg ccggaaacgc cgaggcgcaa gccacgaccg tcgatcggct gcagttgttc
     5221 aagggcgaga tccccaacgt cgacttcatc gtgggcggca acgaggacgt gccgggcacc
     5281 atgggcctgc tgggacgcaa tatcctgtcg gcggtcgaca cggaatacga cctcgcccac
     5341 gggatgatcc gcctggtcat ccccagcgac gagtgcgaag agtccgtcat ggcctactgg
     5401 gccgcgggca tgcaggtggc cgagctccgc ctgctgcgcg ccttcaacga gcggcagcca
     5461 tcgatccggg ccaaggtcaa gatcaacgac gcgacggtca ccgcactgtt cgacaccggc
     5521 gccatgacgc tgctgtcgct gcgcgccgcg cactgggccg ggctgaagga cggcgacctg
     5581 agtcccaatg gaaggcacta cggcatcggc accgacagcg tgaattcctg gaccgcgaag
     5641 atcgcgaagg tggacttcgg gggcgaggcc atcatgaaca acacgctcgt cgtggacgac
     5701 tacgacatgg ccgacaacga catgctggtg ggcatcgact tcttcctgtc gcaccgcatc
     5761 tacgtgtcca agcggcagtc gaagatgtac ttcacgtacg ccggtggccc cgtcttcgca
     5821 cgcaacaagg gcgagctcgc cgacacggcg gccgccgact ccgccgcggc gtcgggcgcc
     5881 gcaaccatga cggcggacgc cctggcgcgg cgcggcgagg catcgctgtc gcgcggcaac
     5941 gtggccagcg cgctggccga cctcgaccag gcctgcgcga tggagcctgt caacgcgagc
     6001 catcgctggg ctcgcgcccg cgcgcatctg gcgatgaagg acgtccccgc ggcgcgagcc
     6061 gacctggaca aggcgatcga gctggccccc cggctcgcgg aggcccgcat cgtgcgcgcc
     6121 ggcctgcgcg aagccgctca cgagcgggag gcggccctgg acgacctcgc tgcgctggac
     6181 accgccctgc ccgcgccgtc ggacatccgc cgcggaatgg cgatgctgta tggcgagatg
     6241 cacatgccgg cgcaggcgat cgctcaatgg aaccagtggc tgcccgcgca cgtgcacgac
     6301 atcggtcgtc cggctgcgct caacgcgcgc tgctggagcc gcgtcgagct cggcgtggag
     6361 ctggacaagg cgctggccga ctgcgacgag gcgatcgaca tcgacggcaa gaacgcgtcg
     6421 tacctggaca gccgcgcctg gacgtatctg cgcatgggaa agctcaagaa agccctggcg
     6481 gacttcgacc gcgcgatcga gatcaatccg aagggcgcct ggtcgctgta tggccgcggc
     6541 caggtgcagc tcggtctggg ccaggccgaa cgagcccggg cggacctggc ggcggcgcgc
     6601 acggagcgac ccggcatcga cgcggatgcc aagcgggacg gcctgccaca ggcgccgatc
     6661 gcgccgccgt cgcccgcgtc gccctgaacg cgcgcggcgc tcagctgcgc cgcgccgcca
     6721 tgctgcgcgg tcgcccctgc gcggcgcgcg cgttcgtcag ttcctgcgtc gccgcctcgt
     6781 tggcctgctc gagccactgc agcgcgtcga tgtagccgac gaagtgctcg aggtattccg
     6841 gcgacagttc gcgcatcagc accagcgagc gatgcaccag gtgctgcgag ttcagcggcc
     6901 ccgcgttctc gggcagcgag gcgcgcgact gcgccagccg ctggtcggcg gacaggcgcg
     6961 accacgtgcg cttgaagaac tgcacggtct tttcgtcggc cgcggcgccg gtgaccgccg
     7021 gcgcggcggc gcccggcgct gcggccacgg gtcgcgcgcc gggaacggcg ccgggtgccg
     7081 cgccgcgacg tcgagccgcg tgcgccacga gcgcggccag cgcgccaagg ccggcggcag
     7141 gtgcgacggg ccgggccggc ggggcgcgtt cgggcagttc cgacaaggat tgcacgatcg
     7201 cggcgacgcg ctcgtcgatc cttcgccgtg cctcgcccgc atgcgcgttc gcgcggcgcg
     7261 ccagcgcctc ggcgaggcgc aagcgcaccg ggtcgcgcgc ctgcgcgtcg cgcacgggcg
     7321 cctgcgcgtc gggcgcgggc tcgggcgcga cgccgtcggc ggcggtcgtc tcagccatgc
     7381 gcggcgctcg cggcgacggg gacgctggcc gcaccggcca cggggcgcgc gcgcgccgtg
     7441 cgcggcgtgg gcgccatctc cacgcgccgg ttcctggcgc ggccgtcggc ctgcgcgttg
     7501 tcggccaccg gctgctcggc gccgaacgcc gcggcgaaga tgtccgacga cggcacgccc
     7561 gcctcgatca gcgcgcgcgt cacggtcagc gagcgctgcg ccgacagctc ccagttgtcc
     7621 gcgaactggt ggttgccgcc gcgcacctgg cggtcgtcgg tgaagccgct gaccatcagg
     7681 atctcgtcgc gcgccttcag gtaggtggtg accgggccgg ccagttgcct gagcagcgcg
     7741 aggccctcgg gctgcagctc cgcggaattg aacgcgaaca gcacgctgcc gttgatgtcg
     7801 atgcggccgt ggtcgatggt gacgcggccg gcggccagcg gcacggacag ggccttctcg
     7861 agcgcggcgc ggcgctgctc ctcgagctgg tgctggcgga tctcgtcctg cagccgcgtc
     7921 acgagttcca gctgcgacgc cagcgcgaac agcaggatca gcacgaacgc gcccagcagg
     7981 cccgacatca ggtcgccgaa caccgcccac acgggcgccg acgcgtcggc ggcgccgtcg
     8041 acatcgatgt cgtccatgtt catcgcacga cctccggcct gcggccttcg gagcgagcgc
     8101 ctgctggcgg ccgggcggga gtcataaggc aacctccgtc ctgcggactg cggtgcgagc
     8161 cccttcgggc ggccgggcgg cgctcacgct gccgcctcct cggcacggcg gccggccagg
     8221 cgctgcagtt cctcgatgat ctgcttctgc gacaggatgc tcaggtcgat cacctcgcgg
     8281 gcctgcgcca cgtagtaggc gagctgctcg tcgctgcgct ccatctgcgc gcccagcgcc
     8341 acgtcgatgc gctccaggtg cgcgcccagc cggtcgcccg actccgtgaa gcgctgcacc
     8401 gcgacgccga aggcctcgcc caggctcgcg acctcgacgg cgctgccggt gacctgcgcc
     8461 gcgacgtcga ccagcttgcc ggcctcggcc tgcacctgcg cctggaagcg ctcgccgacg
     8521 cgctcgagca cctcggccga agacgtcacg agcgcgtcga tcgcgccccg ctgctcgccg
     8581 gcggcgcggt tgacgccgtc cagcagcgtg cccagggtct cgaggatgcg ggcgcgttcg
     8641 tccagcatcg tgttgtcgcg cgccatgctg tcggccagct tctggcgcag ctcggagatg
     8701 acctcggccg ccgcgcgcgg cgcttccgag gccgccgcga ccaggcggcc gatctcggcg
     8761 atcgtcgatc gcgcctgcgt ctcggcctgc gcggtgatgt cgcgcgccgt gcgctccatg
     8821 gcctcgatca cgcgctgctg ctgctcgagc gactgcgcgc cggcctgctg ccactgcgcc
     8881 tgcagcgacg cggccatcgc ctccagcgag ctcgccatcg cgtcgacgcg ctgctggtcg
     8941 cgcgcggccg acgtggcctg cagctcggcg tgcgcctgct gcacggtctg cagcagcgac
     9001 gcggagtgct gctcgaaggt cgcggccgcg gtcgacagcg accgctcggt cgatgccgcc
     9061 agggccgcgc cggcgtcctg ctggcgcgcg acggcgtcgg cccaggcctg ggtcacgcgc
     9121 gcgacggccg cgtccaggcg cgcgccggcg ccttcgagct gggtggccgt cgcgccggcg
     9181 atcgcctgct gcgtggccgc ggtctcgcgc gcgagcgtcg ccagcgtcgt cgccatctcg
     9241 ccctgccacc cggcatggcg ctgcgcgacc tggtccagca acgcggtgga gcgctgctcg
     9301 aaggtctggg cgaagcgcgc gtgcgaggcc tcgacgtcgg ccgccagcga ctgcgcggcc
     9361 tgctcatggc gtgcgagcag cgcctgccac gattcggcgc tgttggcagt gctcgccgcg
     9421 aagctcgacg acagctgcgc cacgtgctcc cgcatcgtgc cggccagcgt cgcgtgcagc
     9481 gtggcggtct cgcgcgcgat gccggccagc gtggcctcgg ccacgggacg gatcgtctct
     9541 ccggcgatgc gcgcaccctc ggtcaggctc gcctgcagcg tgcggtcgac cgactgcgcg
     9601 agcgcgacgt acgcgatctc ggcctggccc tggaagcgct cctggctcgc gagcaggcgt
     9661 gcctgcgacg cctcggcctg gcgctccatc gtcgccatca tcgccttgag gccgtcgacg
     9721 agcgccggca tcagttcggc ctggcgctgc agcagggcca gctgctcctc gcgctggtgc
     9781 gacaccgaga acgggcgcag cgtcgtggcg atgcgcgcat ccaggcgctg cgccaccgcg
     9841 acgcgctcgc gccgcgcgag cgccgacagc aggcccagcg ccgcggatgc cgcgaccccg
     9901 gcgacggacg tgccgaacgc caggcccagg cccttgaccg gcgcgatcag cgagttgcgc
     9961 acggcgacga ggtcggtggc cgtctcgagc gcggcgccgg tgccgcgcag cgtgaggatc
    10021 atgcccagga aggtgcccag catgcccagc agcaccagca ggccggcgag catcggcgcc
    10081 agcgcgggac cgggcaggcc gacgggctcg ccctcgacgc ggcggcgcac ggcgttgcgc
    10141 aggccggccg gcagctgctc cagccagggt gccaggctgg cgggcgtcgc ggcgagcgcg
    10201 tcgacggccg tggcgagcgc ggacgtggcc tggcggaagc gccgcagctc cagcacgccc
    10261 atggcgtaga acgcgccgat cagcagcgtg agcacgagcg cgagcgggtt ggccagcagg
    10321 tagcccgcgc cgacccaggc gatgacggcc aggccggtga cggcggcggc gtggcggagg
    10381 aattggttca tgtgcgatgg agcgactggc ggcaggcctg gagcaggccc tcgcagggtt
    10441 gcaggcggag atcgagttcg gcgagcagca ggccccgcat ctcctggccg aagcgctcga
    10501 gccacgggcc cgcgggcgcg tcggcgtcgg ggccgcgcaa tcggtcgaag cgcgcctcga
    10561 gccgtcccgc gacggtggcc agcagcgcgc gttcctgcgc gccgacgacc tgctccatca
    10621 cggtgtcgag ctcggcgagc ttggccgccc tggatgaacg atcggccagc gtggagcgca
    10681 ggcggcggcg cagcggcacg atctgcgtct ccatcgccag ctggcacttc gtgtagcgct
    10741 ggcgcagggt ggcgaagtcg acgggcatct cggcgggcgc gacggcgtcc ttcagcgggc
    10801 gcacgatgcg gcgcgcggct gcggcgccgc ccgtgggggc atcggcgatc gccttggcga
    10861 gcgaggcctt cacgcgggcg aactcgcgct cgtccgcggc cagcgcgcct tggccgccga
    10921 acgtcggggc gtcggcgcgc gcggacgcgt cacccagcgc cgccgacagc gagaatgcat
    10981 gggtccagtc gaaccatcgg gcgagccgct gcgcgaacgc gtcgggcgcc gagggcacgg
    11041 acgcgtcgtc gagagcggcc aacgcgcgga tgaacgtcga gccggtgaga gggatgcggc
    11101 gcgcttcgcg cgcgaggctg ccggacactg tcgcagtgga aaaaagggcg cagtctactc
    11161 tcgttccgtc atcgaaacgg cgggcgattt gtggtacgcg cgtccactgt cgagcaagat
    11221 cggaaaggct gttaccggca gccgacgccg cggtgagtcg cggtagggc
//
LOCUS       DATCRO010000804         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_953681, whole genome shotgun sequence.
ACCESSION   DATCRO010000804 DATCRO010000000
VERSION     DATCRO010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953681"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1662)
                     /locus_tag="VIN75_21925"
     CDS             complement(64..1662)
                     /locus_tag="VIN75_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEY8976889.1"
                     /translation="MNERHALRRRGLLAAASMLPLGAWPASALRAAGTANRHIELDLA
                     KATQPLDRFFNDCVGSDYPGTLYRPDTLAQLKTAVDELGFRRLRFHAIFHDVLETVTR
                     DAQGALVFDFGGIDKLYDALLERGIRPLVELGFTPKALATSDSKIFWWAGNTSHPKPD
                     EWVALIDAFVRHLLQRYGADEVRRWEFEVWNEPNLDGFWEHADQAAYFDLYTRTAKAI
                     KAIDPRLAVGGPSTAGAAWVPEFLAHAKATGTPVDFITTHAYGVDAGFLDEFGVEDRQ
                     LSPSPDAIIGDVRRVRAQIEASHLPGLPLYFTEWSTSYNPRDKVHDSYMSASYVLSKL
                     RQSRGLAQSMSYWTFSDLFEEPGPPDAAFHGGFGMMTRDGLRKPVWFAYKYLAALAGR
                     EVPVSDPSCWASTDAGAVRAVIWDWRNPDQRVSNRSFFGKPQPARAIAPASVALSGLA
                     PGRYRLALHRTGYEANDAHTAYLRMGEPKDLDAAQLRRLQDLTRDLPESSREVRVGSS
                     GRLALQVALRTHDVVLLRLDPLRG"
     gene            complement(1863..2117)
                     /locus_tag="VIN75_21930"
     CDS             complement(1863..2117)
                     /locus_tag="VIN75_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976890.1"
                     /translation="MIVKTIGHVSSEWRGRDIFLRCNNCGQTEQIADYAGGGKPRLFG
                     WSEAGRGQFGFSKCHQCLMVYDRSADEPAEVRGVRRPVYR"
     gene            complement(2362..3207)
                     /locus_tag="VIN75_21935"
     CDS             complement(2362..3207)
                     /locus_tag="VIN75_21935"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY8976891.1"
                     /translation="MLEDSALTQALGTALARLLRPLVRLMLRHSFSYSAFESVAKRVY
                     AECAMEDFALPGRKPSVSRAAILTGLTRKEVNTLLTEPWSGANADSTHYNRAARVVTA
                     WVRDSRFTGIDGTPRALAMEGADGFSELVRLHGGDVPTRAVLDELVRLGSVRPRHDGS
                     VELVQRGFVPNENMVAKISFLGTEVADLVETITFNIEQPNPQAARYQRKVMHTGIPPE
                     ALPAFRALSGKQAQKVLEGLDAWLAEHDLSGVPEEDWGTTARVGVGIYYFEEIVPAPQ
                     ADSTP"
     gene            3391..3780
                     /locus_tag="VIN75_21940"
     CDS             3391..3780
                     /locus_tag="VIN75_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEY8976892.1"
                     /translation="MDYLAIRSFHETSVALSVTGFALRGAAGLAGARWVRGRVARTLP
                     HVIDTALLASALMLVAMLRLNPLATPWLLAKLCGLVVYIGLGVVALRPTIARPVRAAA
                     WVGALAVVAWIVSVALTKTPWGFLAAP"
     gene            complement(3792..>4026)
                     /locus_tag="VIN75_21945"
     CDS             complement(3792..>4026)
                     /locus_tag="VIN75_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HEY8976893.1"
                     /translation="AGLMPGRAADDPLPAASASIAPVAAASAPALPASTATATVEPPA
                     PHTAWLWAALLGGLAVIGSMAWSLLRKPRATQA"
BASE COUNT          563 a         1356 c         1477 g          630 t
ORIGIN      
        1 gggtgcggaa ctggcctgga cggcctgaac gggaggcggc ggccgcgatg gccgccgcgc
       61 gcctcacccg cggagcgggt ccaggcgcag cagcacgacg tcgtgcgtgc gcagcgccac
      121 ctgcagcgcg aggcgtcccg aggagccgac gcgcacctcg cgcgacgact ccggcaggtc
      181 gcgtgtcagg tcctgcagcc gcctgagctg cgccgcgtcc aggtccttgg gttcgcccat
      241 gcgcaggtac gcggtgtggg cgtcgttggc ctcatacccg gtgcgatgca gcgcgagccg
      301 gtagcggccc ggcgcgagcc cggacagcgc gaccgaggcc ggcgcgatcg cgcgcgccgg
      361 ctgcggcttg ccgaagaagg atcggttgct gacccgctgg tccggattgc gccagtccca
      421 gatcaccgcg cggaccgcgc ctgcgtcggt cgaggcccag caggacggat cggacaccgg
      481 cacctcgcgg cccgcgagcg cggccaggta cttgtaggcg aaccacaccg gcttgcgcag
      541 gccgtcgcgc gtcatcatgc cgaagccgcc atggaaggct gcgtcgggcg gcccgggctc
      601 ctcgaacagg tcgctgaacg tccagtagct catcgactgc gcgagaccgc gcgactgccg
      661 cagcttggac agcacgtacg aggcgctcat gtagctgtcg tgcaccttgt cgcgcgggtt
      721 gtagctggtg ctccactcgg tgaagtacag cggcaggccc ggcaggtgcg acgcctcgat
      781 ctgggcgcgc acgcggcgca cgtcgccgat gatcgcgtcg ggcgagggcg acagctgccg
      841 atcctcgacg ccgaattcgt ccaggaagcc cgcgtcgacg ccgtaggcat gcgtggtgat
      901 gaagtcgacc ggcgtgccgg tggccttggc gtgcgccagg aactcgggca cccaggccgc
      961 gcccgccgtc gacgggccgc cgacagccag gcgcgggtcg atcgccttga tcgccttggc
     1021 ggtgcgcgtg tagaggtcga agtaggccgc ctggtcggcg tgctcccaga agccgtcgag
     1081 gttgggttcg ttccagacct cgaactccca ccggcgcacc tcgtccgcgc cgtagcgctg
     1141 cagcaggtgg cgcacgaagg cgtcgatgag cgcgacccac tcgtcgggct tcggatggga
     1201 ggtgttgccg gcccaccaga agatcttgct gtcggaggtg gccagtgcct tgggcgtgaa
     1261 gccgagctcg acgagcggcc ggatgccgcg ttccagcagc gcgtcgtaca gcttgtcgat
     1321 gccgccgaag tcgaacacga gcgcgccctg tgcgtcgcgg gtcaccgtct cgagcacgtc
     1381 gtggaagatg gcgtggaagc gcaggcgccg gaagccgagt tcgtcgacgg ccgtcttgag
     1441 ctgcgccagc gtgtcgggcc ggtacagcgt gccagggtag tccgaaccga cgcagtcgtt
     1501 gaagaagcgg tccagcggct gcgtcgcttt tgcgaggtcg agctcgatgt gacggttggc
     1561 agtgcccgcg gcgcgcaagg cgctcgcggg ccaggcgccc aggggcagca tcgatgcggc
     1621 ggcgagcagg ccgcggcgcc gcaatgcgtg gcgttcgttc atttcggatg tctccagacc
     1681 gggttgcgaa cgtcacgcac gcacggcgcc cggagtggtt cgatccgaaa atgctagatt
     1741 cgtgcgggca tcaaactaat acacagaaac ccggcccgcg gatgcttgcg gcggctcggg
     1801 caggggcgag tgccggacag cgcctgcggc gaacgatccc tggcgacgac cgcgcctggc
     1861 gcttaccgat agacgggcct gcgcacgccg cgcacctcgg ccggctcgtc cgcgctgcgg
     1921 tcatacacca tcaggcattg atggcacttg gagaacccga actggccgcg gccagcctcg
     1981 gaccagccga acaggcgggg cttgccgccg ccggcgtagt cggcgatctg ttcggtctgg
     2041 ccgcaattgt tgcagcggag aaagatatcg cgtccgcgcc actcggacga cacgtgtcca
     2101 atcgtcttca cgatcaaccg ccctgctcaa atgtggccgc cctcgcgtgc cacggtgctc
     2161 gaaagcttcc tgacgaagcc tgatgtcgcc tcggcctgct ctgtcggctg gcctgtgcgg
     2221 actgcaccct cttgcacgga ttgtggccct gttgtcttgc gctgtcttga caggaaacgt
     2281 ggggaattgc cgtttccgga aagaacatgc atccgcgtct cgccccgttc ggctgcagcc
     2341 tgtcgttcgc ccgccggtgc ctcagggcgt cgaatccgcc tggggggcgg ggacgatctc
     2401 ctcgaagtag tagatgccca cgccgacgcg cgcggtggtg ccccagtcct cctcggggac
     2461 gccggacagg tcgtgctccg ccagccaggc gtcgaggccc tcgagcacct tctgcgcctg
     2521 cttgccgctc agcgcgcgga aggccggcag ggcctcgggc gggatgccgg tgtgcatcac
     2581 cttgcgctgg tagcgcgccg cctgggggtt cggctgctcg atgttgaagg tgatggtctc
     2641 gaccaggtcg gcgacctcgg tgcccaggaa cgagatcttg gccaccatgt tctcgttggg
     2701 gacgaagccg cgctgcacca gctccacgct gccgtcatgg cgcgggcgca cggagcccag
     2761 gcgcacgagt tcgtccagca cggcgcgggt ggggacgtcg ccgccatgca gccgcacgag
     2821 ttcgctgaag ccgtccgcgc cttccatggc cagggcgcgc ggcgtgccgt cgatcccggt
     2881 gaagcgcgag tcgcgcaccc acgcggtgac gacgcgcgcg gcccggttgt agtgcgtgct
     2941 gtccgcgttc gcgccgctcc acggctcggt gagcagcgtg ttgacttcct tgcgtgtcag
     3001 gccggtgagg atcgccgcgc gcgagaccga gggcttgcgg ccgggcagcg cgaagtcctc
     3061 catcgcgcat tcggcataga cgcgcttggc caccgattcg aacgcgctgt acgagaacga
     3121 gtgccggagc atcagccgca cgagcgggcg aagcagccgg gcgagcgccg tgccgagtgc
     3181 ttgcgtcaac gcggagtctt cgagcatgcc ggaattatgg aacgtcggcg gcggcgcgcc
     3241 caatgcacgt ggcgtggatg cgggcccgca acgtggtgcc gatggaactg cgcggcgtgt
     3301 cggcgcaaat ccctgttcat ctccggccgc cgtcgacgcc gtgcgctacc ctcgacccac
     3361 tccgcgacac cctgcgcacg ggccgattca atggactacc tcgcgatacg aagcttccac
     3421 gaaacgagcg tcgccctgtc ggtgacaggc ttcgcgctgc gcggcgcggc tggcctggcc
     3481 ggtgcacgct gggtccgcgg ccgcgtcgcc aggaccctgc cgcatgtgat cgacacggca
     3541 ctgctggcgt cggcgctgat gctcgtggcg atgctgcggc tgaatccgct tgcgacgccg
     3601 tggctgctcg ccaagctgtg cgggctcgtc gtctacatcg ggctcggtgt cgtggccttg
     3661 cgcccgacga tcgccaggcc ggtgcgcgcc gcggcctggg tgggcgcgct ggcggtggtg
     3721 gcgtggatcg tgtcggtggc cttgaccaag acgccttggg gctttcttgc cgcgccgtag
     3781 gcggcctcgc gtcaggcctg cgtggcgcgc ggcttgcgca gcagcgacca ggccatcgag
     3841 ccgatcacgg ccagtccgcc cagcagcgcg gcccagagcc aggcggtgtg cggcgcgggc
     3901 ggttcgacgg tcgcggtggc ggtcgatgcg ggcagggcgg gtgcgctggc ggccgccacg
     3961 ggcgcgatcg acgcgctggc cgcgggcagg ggatcgtccg ccgcacgtcc gggcatgagt
     4021 ccggcc
//
LOCUS       DATCRO010000805         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_436764, whole genome shotgun sequence.
ACCESSION   DATCRO010000805 DATCRO010000000
VERSION     DATCRO010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436764"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VIN75_21950"
     CDS             complement(<1..576)
                     /locus_tag="VIN75_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY8976894.1"
                     /translation="MSKTVADVMKLVQENEVKFVDFRFTDTRGKEQHVSVPISHFDED
                     KFVSGHAFDGSSIAGWKGIEASDMLLMPDANTANIDPFFEEPTLILTCDVVEPSDGKP
                     YERDPRSLAKRAEAYMRSSGLGDTAYFGPEPEFFIFDSIKWDVSMKGCSVEIEAEEAA
                     WNTGKNYEHGNKGYRPSVKGGYFPVPPIDAGQ"
     gene            complement(761..1567)
                     /locus_tag="VIN75_21955"
     CDS             complement(761..1567)
                     /locus_tag="VIN75_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY8976895.1"
                     /translation="MNFGALIIGDEILSGRREDKHLARVIAALESRGLALSWARYIGD
                     EPQRIVAEIRAAAASGDVVFSFGGIGATPDDHTRQCAAQALGVPLALHPEAAELITQR
                     GRETAADKGIAYDTTGPDHQRRLNMGVFPAGATLIPNPYNRIPGFSVGRLHFMPGFPV
                     MAHPMLDWLLDTLYAGLARDRALRAMRVRGANESALIPVFERVEAAFPGVRSFSLPSV
                     DHPALGPHIEIGVKGDDPEQVDLAMAALREGAAAAGAIEIDPEMVRKMPQ"
     gene            complement(1564..2487)
                     /locus_tag="VIN75_21960"
     CDS             complement(1564..2487)
                     /locus_tag="VIN75_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY8976896.1"
                     /translation="MKQLFGSFWRAVAYCLHPRALMLSLLPLLVAGVVTLGLGYFYWE
                     AANRAVRGVLEQWSLVNSLLEWISSMLGAGFRTGITQLIVIALTIPVLVAFTLVLVGL
                     WITPAIVGLVSRRRFPTLERRHGASWWVSVAASFGWTIAALLAVVITLPFWLVPGLAL
                     VLPPLIWGWLTAHIMGFDALAEHASRAEREAILRAHRWPLLVMGIVCGFLCGVPSMIW
                     TLGMWVIIAAPFVMVGVIWLYTLIFTFSALWFTHYCLSVLAELRTAESAMLATRRAAS
                     TASDLELVEEVPPHQRFGFDAGPEPEPKDAA"
     gene            complement(2544..3383)
                     /locus_tag="VIN75_21965"
     CDS             complement(2544..3383)
                     /locus_tag="VIN75_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEY8976897.1"
                     /translation="MAMDVVDYEIKGAEMQFVEVELDPGEAAVGEAGSMMFMESGIVL
                     DTVFGDGSQQNSGLWGALVGAGKRLISGANVFTTVFMNQSSQKRRIAFATPVPGKILA
                     VDLRSVGGALICQKQSFLCAARGVSLGIAFQQRLGVGFFGGDGFIMQRLDGDGMAFVH
                     AGGTVERRELQPGETLLIDHGCLVAFTQTVNFEIQYVGGIKTALFGGEGLFFAKVTGP
                     GTVWIQSLPFSRLASRVFAAAPQNGGGGSDRAGASGSGVLGGLAAGGILGGLGSLLSG
                     DDD"
     gene            3601..>3951
                     /locus_tag="VIN75_21970"
     CDS             3601..>3951
                     /locus_tag="VIN75_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8976898.1"
                     /translation="MTTPVQTPAARPARPAVLDLIGNTPLVELTQFDTGCCQLFLKLE
                     SQNPGGSIKDRIGLAMIETAERDGRLKPGGTVIEATAGNTGLGLALVARAKGYRVVLV
                     VPDKMSSEKVLHLKA"
BASE COUNT          657 a         1376 c         1356 g          562 t
ORIGIN      
        1 ctggccggcg tcgatcgggg gcaccgggaa gtagccgccc ttgacgctgg gacggtagcc
       61 cttgttgcca tgctcgtagt tcttgcccgt gttccaggcg gcttcctcgg cttcgatctc
      121 gaccgagcag cccttcatgc tcacgtccca cttgatggag tcgaagatga agaattcggg
      181 ctccggaccg aagtaggcgg tgtcgcccag gcccgacgag cgcatgtagg cctcggcgcg
      241 cttggccagc gagcgcgggt cgcgctcgta gggcttgccg tcgctgggct cgaccacgtc
      301 gcaggtcagg atcagcgtgg gctcttcgaa gaacggatcg atgttggccg tgttcgcgtc
      361 gggcatgagc agcatgtccg acgcctcgat gcccttccag ccggcgatcg acgagccgtc
      421 gaacgcgtgg ccggagacga acttgtcttc gtcgaagtgc gagatcggca cggacacgtg
      481 ctgttccttg ccacgggtat cggtgaaacg gaagtcaacg aacttgactt cgttttcctg
      541 caccaacttc atcacgtcgg cgacggtctt ggacatcaac gggctcctaa tttgtttggc
      601 aagttaccgg gccggcgcac caggggccgg cctgtcagaa cggtatgcga gggggttaaa
      661 agcaggttcc atgccgcacg ttgcgccacg aaaccccggg gaatcggctg ctggacggcg
      721 cggatcgcgc accgccattg tgcatgccat tcgacgcacc tcattggggc attttgcgca
      781 ccatttcggg atcgatctcg atcgccccgg cggcggccgc gccttcgcgc agcgcggcca
      841 tcgccaggtc gacctgctcg ggatcgtcgc ccttgacgcc gatctcgatg tgggggccga
      901 gcgccggatg gtcgacgctg ggcaggctga aggaccgcac gccgggaaag gcggcctcga
      961 cccgctcgaa cacggggatc agcgccgact cgttggcgcc gcgcacgcgc atcgcgcgca
     1021 gggcgcggtc gcgcgcgagg ccggcgtaga gcgtgtccag cagccagtcg agcatcgggt
     1081 gcgccatcac cgggaagccg ggcatgaagt gcagccgccc gacgctgaag ccggggatgc
     1141 ggttgtacgg gttcgggatg agcgtggcgc cggcggggaa cacacccatg ttcaggcgcc
     1201 gctggtggtc ggggccggtg gtgtcgtagg cgatgccctt gtccgcggcc gtctcgcggc
     1261 cgcgctgcgt gatcagttcc gccgcctcgg gatgcagcgc gagcggcacg cccagcgcct
     1321 gcgcggcgca ctggcgcgta tggtcgtcgg gcgtggcgcc gatgccgccg aacgagaaca
     1381 cgacgtcgcc cgaggccgcc gccgcgcgga tctcggcgac gatgcgctgc ggctcgtcgc
     1441 cgatgtagcg cgcccacgag agcgcgaggc cgcgcgattc cagcgccgcg atgacgcgcg
     1501 ccaggtgctt gtcctcgcgg cggccggaca ggatctcgtc gccgatgatc agcgcgccga
     1561 agttcatgcc gcgtccttgg gttcgggctc ggggccggcg tcgaagccga agcgctggtg
     1621 cggcggcact tcctccacga gctccaggtc ggacgccgtc gacgccgcgc ggcgcgtcgc
     1681 gagcatcgcc gattcggccg tgcgcagctc ggccagcacc gacaggcagt agtgcgtgaa
     1741 ccagagcgcc gagaaggtga agatcagcgt gtacagccag atcacgccca ccatgacgaa
     1801 cggggccgcg atgatcaccc acatgcccag cgtccagatc atcgacggca cgccgcacag
     1861 gaagccgcac acgatgccca tgaccagcag cggccagcga tgggcgcgca ggatggcttc
     1921 gcgctccgcg cgcgaggcgt gttcggcgag cgcatcgaag cccatgatgt gggcggtgag
     1981 ccagccccag atcagcgggg gcagcaccag cgcgaggccg ggcaccagcc agaacggcag
     2041 cgtgatgacc accgccagca gcgcggcgat cgtccagccg aacgaggccg cgacgctgac
     2101 ccaccacgag gcgccgtggc gccgttccag cgtggggaat cgccggcgcg agaccaggcc
     2161 gacgatggcc ggcgtgatcc acaggcccac gaggaccagc gtgaacgcga ccagcacggg
     2221 gatcgtgagc gcgatcacga tcagctgcgt gatgcccgtg cggaagccgg cgcccagcat
     2281 cgacgagatc cactcgagca gcgagttgac cagcgaccac tgctccagca cgccgcgcac
     2341 ggcgcggttg gcggcttccc agtagaaata gcccaacccc aacgtcacga cgcccgccac
     2401 cagcaagggc agcagcgaca gcatcagggc ccgcggatgc aggcagtagg cgaccgcgcg
     2461 ccagaaggag ccgaacagct gtttcatcga agcgtgaagg atacgtgagg ggcccgtcga
     2521 gccggatgac gttcaggccg gattcagtcg tcgtcgccgc tcagcaggct gcccaggccg
     2581 cccaggatgc cgcccgcggc caggccgccc agcacgccgc tgccgctggc gcccgcgcgg
     2641 tcgctgcccc cgccgccgtt ctgcggcgcc gcggcgaaca cgcggctggc caggcgcgag
     2701 aacggcaggc tctggatcca tacggtgccc gggcccgtga ccttggcgaa gaacaggccc
     2761 tcgccgccga acaacgcggt cttgatgccg ccgacgtact ggatctcgaa gttgaccgtc
     2821 tgcgtgaacg cgaccaggca gccgtggtcg atcagcagcg tctcgccggg ctgcaattcg
     2881 cgccgttcga cggtgccacc cgcgtgcacg aaggccatgc cgtcgccgtc caggcgctgc
     2941 atgatgaagc cgtcgccgcc gaagaagccc acgcccaggc gctgctggaa cgcgatgccc
     3001 agcgacacgc cgcgcgccgc gcacaggaac gactgcttct ggcagatcag cgcgccgccc
     3061 accgagcgca ggtccacggc caggatcttg cccggcaccg gcgtggcgaa cgcgatgcgg
     3121 cgtttctggc tcgactggtt catgaacacg gtggtgaaga cgttggcgcc gctgatcagg
     3181 cgcttgcccg cgcccaccag cgcaccccac aggccggagt tctgctgcga gccgtcgccg
     3241 aagacggtgt ccagcacgat gccgctttcc atgaacatca tcgagccggc ctcgccgacc
     3301 gcggcctcgc cggggtcgag ctcgacctcc acgaactgca tctccgcgcc cttgatctcg
     3361 tagtcgacca cgtccatcgc catcttcgct cctccgttga ccggccatcg ccggccccgc
     3421 attctccttc aagccgaggc ggcgccaggc ggccgggcgg aactccggga ttccactgtc
     3481 cctagcacgt ccgcatcgga taaagccatg aggcgatcgc atgacgccgc gcgctatcct
     3541 gggccaccgc caactccaac gcgacccggc cacaagccac gcggtcgcgc tcaaagagac
     3601 atgactacgc ctgtccagac gccggccgcg cgcccggccc gtcccgccgt cctcgacctc
     3661 atcggcaaca cgcccctggt cgagctgacc cagttcgaca ccggctgctg ccagctgttc
     3721 ctgaagctgg agtcgcagaa cccgggcggc tcgatcaagg accgcatcgg cctggcgatg
     3781 atcgagaccg cggagcgcga cgggcggctc aagccgggcg gcacggtcat cgaggcgacg
     3841 gccggcaaca ccggcctcgg gctggcgctg gtcgcgcgcg ccaagggcta ccgcgtcgtg
     3901 ctggtggtgc ccgacaagat gtcgtcggag aaggtgctgc acctgaaggc g
//
LOCUS       DATCRO010000806         6125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_976468, whole genome shotgun sequence.
ACCESSION   DATCRO010000806 DATCRO010000000
VERSION     DATCRO010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976468"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VIN75_21975"
     CDS             <1..1357
                     /locus_tag="VIN75_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY8976899.1"
                     /translation="NDWLDHDYIARHVDGWDGLRERALQWTPERAALECGLTAAQIRD
                     FARLYGTTKPAGIRLNYGMQRVRGGGNAARLVALLPCLTGAWRHRAGGMLLSASGWLK
                     DFRNPGFDHAGLLAGRNPRTVNMVTIGDDLHRQSGETLPDGRAFGPRIEALIVYNSNP
                     VAVAPDSRRVAAGFRREDLFTVVLEHFLTDTADHADYVLPATTQLEHWDVHGSYGHTT
                     IVINEPALTPRGQSRSNAAIFRALAQRMGFAEPCFADDDETLAREAFKGPVPFEQLRE
                     VGWARLPLPEAPFADGGFPTPNGKARCDAPGIGLPDYVPPYESVRSAPELAKRFPLAM
                     ISPPARNFLNSTFVNVKSLRAIEHEPILEIAAADAAARGIADGDTVRVFNDRGDYRCV
                     ARVSERARAGVVHGLGVWWRKYGLDGTNVNELTHQKLTDLGRAPSFYDCLVEVERSGT
                     T"
     gene            1360..2538
                     /locus_tag="VIN75_21980"
     CDS             1360..2538
                     /locus_tag="VIN75_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY8976900.1"
                     /translation="MRWWPRRRAARWGLASALGVAGVVVAALGTLCATSGCASIDYLG
                     QSASGHVRLLAAARPVQEAIADPATPEALRKRLELTQRMRQFAVTELHEPDNGSYRSY
                     ADLKRSAAVWNVVAAPELSLKLQTWCFPIVGCVGYRGYYDLASANEAAASLKAEGLEV
                     DVYEVPAYSTLGWMNWIGGDPLLNTFVQWPEGELARLIFHELAHQVAYAPGDTTFNES
                     FATAVERIGGQRWMDANASPEVREKQALTEVHRHDFHVLVRAWRAKLDEMYRSDLPDA
                     EKRRRKAELYAGMRADYEALKRDKWGGYKGYDHWFATANNAALGAQAAYDDQVGAFER
                     LFVAEGSDFDRFYAEVKRMAALTKPERDAAMARYRDPEPLPAPVVAPATPASSASVSG
                     "
     gene            2574..2966
                     /locus_tag="VIN75_21985"
     CDS             2574..2966
                     /locus_tag="VIN75_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY8976901.1"
                     /translation="MADLHLHREHTLGLARARKVAMKWAEDVESKLDMECTMIEGDDE
                     DVLEFTRSGVDGRMTVAATFFDLEAKLGFLLKPFLASIEAEVRKQLEEALAREEATAG
                     KPAAKAAAAKAPKPADKAAAAKAPARKK"
     gene            complement(3111..3365)
                     /locus_tag="VIN75_21990"
     CDS             complement(3111..3365)
                     /locus_tag="VIN75_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEY8976902.1"
                     /translation="MSNLTEQFEQAVADSKNLPERPDNMTLLKIYALYKQATEGDVDG
                     KRPGFTDMVGRAKWDAWNELKGQSSDEAKQAYVDLIEDLK"
     gene            3519..4922
                     /locus_tag="VIN75_21995"
     CDS             3519..4922
                     /locus_tag="VIN75_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY8976903.1"
                     /translation="MSRVDTAWLRMDTEANLMMIVGVWLIEPQLSLAELRERVGTALL
                     QYGRFRQKVVEDALGAQWVDDDHFDLAAHVVPEKLELAKDQSPLEALKHYVGELATQP
                     LNPSRPLWQFHLFEDFETGRTALVARIHHCIADGIALISVMLSITDGGKSPPSRRKRE
                     AEDDENDWIADYLIKPASDLAVKAIGVYGAGMGKGIEMMADPAAPLNGSLEMARIGAQ
                     VVSDVAAMALMPDDSSTSLKGKATPGKRVAWGEPLPLPEVKAIGHALGGSINDILLAS
                     VAGAIGSYLRDKGEDPAGKEIRAMVPVNLRPLEKAWQLGNRFGLVPLVLPIGIANPID
                     RLAAVRARMQELKGGYQPLLAFALLSVAGVLIKPVQHALLGLFAKKATAVMTNVPGPS
                     VPLRICGRTLKQVMFWVPASGDIGMGVSILSYGGGVQFGLITDARMCPDPDEIVQRFR
                     PEFEQLLYLAMMLPWER"
     gene            complement(4990..5766)
                     /locus_tag="VIN75_22000"
     CDS             complement(4990..5766)
                     /locus_tag="VIN75_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976904.1"
                     /translation="MAKWTAFPYDAADYTYDAAALKKHWARLHKGDAEPFPKDDAVLA
                     AWAAFHAGDFRKAYDAGLKAGDGGIVVANKAQSIYANYLESSEKTKLAMFMEVAERAE
                     AQAKANPKLANAHYWQAYALGRYSQGISVAKALTQGLGAKVKNALETTIALAPKHADA
                     HIALGAFHAEVIDKVGKLLGKTQGADTATGLKMFKEALSLNPDSAIARVEYANGLVML
                     EGDKRMKEAEQLYAEAAECKPMDAMERLDVEMAREELEDE"
     gene            complement(5807..>6125)
                     /locus_tag="VIN75_22005"
     CDS             complement(5807..>6125)
                     /locus_tag="VIN75_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8976905.1"
                     /translation="HIETQLQEVLEDKTQAIRGLVNGMSEAGHLAIDPREVDALAQSM
                     VVVLTYWLSYEYVREPRKALEPDQAGATLARGAWHVLSLLTPYLEPAAREHLQRLAGA
                     YKS"
BASE COUNT          918 a         2092 c         2172 g          943 t
ORIGIN      
        1 caacgactgg ctggaccacg actacatcgc ccgccatgtc gacggctggg acggcctgcg
       61 cgagcgcgcg ctgcagtgga cgcccgagcg cgcggccctc gaatgcggcc tgacggcagc
      121 ccagatccgc gacttcgcac ggctgtacgg cacgaccaag ccggccggca tccgcctgaa
      181 ctacggcatg cagcgcgtgc gcggcggcgg caacgcggcg cgcctggtgg cgttgctgcc
      241 gtgcctgacc ggcgcgtggc gccaccgtgc cggcggcatg ctgctgtcgg cctcgggctg
      301 gctcaaggac ttccgcaacc cgggcttcga ccacgccggg ctgctggccg gccgcaaccc
      361 gcgcacggtc aacatggtca ccatcggcga cgacctgcac cgccagtccg gcgagacgct
      421 gcccgacggc cgcgccttcg ggccgcgcat cgaggcgctg atcgtctaca acagcaaccc
      481 ggtggccgtg gcgcccgatt cgcgccgcgt ggcggcgggc ttccggcgcg aggacctgtt
      541 cacggtggtg ctcgagcact tcctgaccga caccgccgac cacgccgact acgtgctgcc
      601 cgccaccacg cagctggagc actgggacgt gcacggcagc tacggccaca ccaccatcgt
      661 catcaacgag ccggcgctca ccccgcgcgg ccagtcgcgc tccaacgcgg cgatcttccg
      721 cgcgctggcg cagcgcatgg gatttgccga gccgtgcttt gccgacgacg acgagacgct
      781 ggcgcgcgag gcgttcaagg gcccggtgcc gttcgagcag ctgcgcgagg ttggctgggc
      841 gcggctgccg ctgcccgagg cgccgttcgc cgacggcggc tttccgacgc cgaacggcaa
      901 ggcgcgctgc gacgcgccgg gcatcggcct gcccgactac gtgccgccct acgagagcgt
      961 gcgcagcgcg cccgagctcg cgaagcgctt cccgctggcc atgatctcgc cgccggcgcg
     1021 caacttcctc aactccacgt tcgtcaacgt caagagcctg cgcgcgatcg agcatgagcc
     1081 catcctcgag atcgccgccg ccgacgccgc cgcgcgcggc atcgccgacg gcgacaccgt
     1141 gcgcgtgttc aacgaccgcg gagactaccg ctgcgtcgcg cgcgtgagcg agcgcgcccg
     1201 cgccggcgtc gtgcacgggc tgggcgtgtg gtggcgcaaa tacggcctgg acggcaccaa
     1261 cgtcaacgag ctgacgcacc agaagctgac cgacctcggg cgcgcgccgt cgttctacga
     1321 ctgcctcgtc gaggtcgagc gctcggggac gacctgacga tgcgctggtg gccgagacga
     1381 cgagcggccc gctggggcct ggcgagcgcg ctgggcgtcg cgggcgtcgt ggtggcggcg
     1441 ctcggcacgc tgtgtgcgac ctcgggctgc gcgtcgatcg actacctggg ccagtcggcc
     1501 agcggccacg tgcgcctgct cgcagcggcg cggccggtgc aggaggccat cgccgatccg
     1561 gcgacgcccg aggcgctgcg caagcggctg gagctcaccc agcgcatgcg gcaattcgcc
     1621 gtcaccgagc tgcatgagcc cgacaacggc agctaccgct cgtacgcgga cctcaagcgc
     1681 agcgccgcgg tctggaacgt ggtggcggcg ccggagctgt cgctcaagct ccagacctgg
     1741 tgcttcccga tcgtgggctg cgtcggctac cgtggctact acgacctggc cagcgcgaac
     1801 gaggccgcgg cgtcgctgaa ggccgaaggc ctggaggtcg acgtctacga ggtgccggcc
     1861 tattccacgc tgggctggat gaactggatc ggcggcgatc cgctgctcaa caccttcgtg
     1921 cagtggcccg agggcgagct ggcgcggctg atcttccacg agttggcgca ccaggtggcc
     1981 tacgcaccgg gcgacaccac gttcaacgag tccttcgcca ccgccgtcga acgcatcggc
     2041 ggccagcgct ggatggacgc caacgcatcg cccgaggtgc gcgaaaagca ggcgctgacc
     2101 gaagtgcacc gccatgattt ccacgtgctg gtgcgcgcct ggcgcgccaa gctcgacgag
     2161 atgtacagga gcgacctgcc ggacgcggag aagcgaaggc gcaaggccga gctctacgcc
     2221 ggcatgcgcg ccgactacga ggcgctcaag cgcgacaagt ggggcggcta caagggctac
     2281 gaccactggt tcgccaccgc caacaacgcg gcgctcggtg cgcaggcggc ctacgacgac
     2341 caggtgggcg cgttcgagcg cctgttcgtc gccgagggca gcgacttcga ccgcttctac
     2401 gccgaggtca agcgcatggc cgcgctgacc aagcccgaac gcgacgccgc gatggccagg
     2461 taccgcgatc cggagccgct gccggcgcct gtcgtcgcgc ccgcaacacc ggcgtcgtcg
     2521 gcgtccgtct ccggctgaat ctggccgaga attccggaaa acgaggagac cggatggccg
     2581 acctgcacct gcatcgagaa cacaccctgg gcctggcccg cgcgcgcaag gtcgcgatga
     2641 aatgggccga ggacgtggag agcaagctcg acatggaatg caccatgatc gagggcgacg
     2701 acgaggacgt gctcgagttc acgcgctcgg gcgtggacgg ccgcatgacg gtggcggcca
     2761 cgttcttcga cctcgaggcc aagctcggct tcctgctcaa gcccttcctc gcctcgatcg
     2821 aggcggaggt gcgcaagcag ctcgaggagg cgctggccag ggaagaggcc acggcgggca
     2881 agccggcggc caaggccgcg gcggcgaagg cgcccaagcc ggcggacaag gccgcggcag
     2941 ccaaagcacc ggccaggaag aagtagcgtg tcatcctgcg cgcagtcgca ggatcgacgt
     3001 gtcaggatgc cgaggtggac cctgcgactc cgcttcgcta cgcgcaggat gacggcttgg
     3061 cgtgcgatgc atgaccgctc ggcgcgggac gcctcgtcac ggcgcagtgc ttacttgaga
     3121 tcttcgatca ggtcgacgta agcctgcttg gcctcgtccg acgactggcc cttcagctcg
     3181 ttccaggcgt cccacttcgc gcgccccacc atgtcggtga aaccggggcg cttgccgtcg
     3241 acgtcgcctt cggtggcctg cttgtagagc gcgtagatct tcagcagcgt catgttgtcc
     3301 gggcgctcgg gcaggttctt gctgtcggcg acggcttgtt cgaattgctc ggtgaggttg
     3361 ctcatggcgg ttgttccgga gtgggtggaa gtcaggcttg acggggcgga tgttaaaacc
     3421 caatctcgcg agccgggccg ggagaatgcc gggacgccac tctagaccga atcggatgga
     3481 tccccagggg agatgacttg agcgcaaacg tggaacgcat gtcccgggtc gatacggcct
     3541 ggctgcgcat ggataccgag gccaacctga tgatgatcgt cggggtctgg ctgatcgagc
     3601 cgcagctgtc gctggccgag ctgcgcgagc gggtgggcac cgcgctgctg cagtacgggc
     3661 gcttcaggca gaaggtggtc gaggacgcgc tcggggcgca atgggtagac gacgaccact
     3721 tcgacctggc ggcgcacgtc gtgcccgaga agctcgaact tgcgaaggac cagtcgccgc
     3781 tggaggcgct caagcactac gtcggcgaac tcgccaccca gccgctgaac ccgagccgcc
     3841 cgctgtggca gttccacctg ttcgaggatt tcgagaccgg ccgcaccgcg ctcgtggcgc
     3901 gcatccacca ctgcatcgcc gacggcatcg cgctgatctc ggtgatgctg tcgatcaccg
     3961 acggcggcaa gtccccgccg tcgcgccgca agcgcgaggc cgaggacgac gagaacgact
     4021 ggatcgccga ctacctgatc aagccggcgt ccgacctcgc ggtcaaggcg atcggcgtgt
     4081 acggcgcggg catgggcaag ggcatcgaga tgatggccga cccggctgcg ccgctgaacg
     4141 gcagcctcga gatggcgcgc atcggcgcgc aggtggtgtc cgacgtcgcc gcgatggcgc
     4201 tgatgcccga cgactcgtcc accagcctga agggcaaggc cacgccgggc aagcgggtgg
     4261 cgtggggcga gcccctgccg ctgcccgagg tcaaggcgat cggccacgcc ctcggcggct
     4321 cgatcaacga catcctgctg gccagcgtgg ccggggccat cggcagctac ctgcgcgaca
     4381 agggcgagga tccggcgggc aaggagatcc gcgcgatggt gccggtgaac ctgcggccgc
     4441 tggagaaggc ctggcagctg ggcaaccgct tcggcctggt gccgctcgtg ctgcccatcg
     4501 gcatcgccaa cccgatcgac cgcctcgcgg ccgtgcgggc gcgcatgcag gagctcaagg
     4561 gcggctacca gccgctgctg gcgttcgcgc tgctgtcggt ggccggcgtg ctgatcaagc
     4621 cggtgcagca cgcgctgctg ggcctgttcg ccaagaaggc cacggcggtg atgaccaacg
     4681 tgccgggccc gtcggtgccc ctcaggatct gcggccgcac gctcaagcag gtgatgttct
     4741 gggtgcccgc gtcgggcgac atcggcatgg gcgtgagcat cctcagctac ggcggcggcg
     4801 tgcagttcgg cctgatcacc gacgcccgca tgtgcccgga ccccgacgag atcgtccagc
     4861 gcttcaggcc ggagttcgag cagttgctct atctggcgat gatgttgccc tgggagcgtt
     4921 gatcgagcga tcggcagggg tgtcatcctg cgcggggtcg caggatcccc gccggcctgc
     4981 caggcagtct cactcgtcct cgagctcttc cctggccatc tcgacgtcga gccgctccat
     5041 cgcgtccatc ggcttgcact cggcggcctc ggcgtagagc tgctcggctt ccttcatgcg
     5101 cttgtcgccc tcgagcatga ccaggccgtt ggcgtattcg acgcgggcga tggcggagtc
     5161 ggggttcagc gacagcgcct ccttgaacat cttcaggccg gtggcggtgt cggcgccctg
     5221 ggtcttgccc agcagcttgc cgaccttgtc gatcacctcg gcgtggaagg ctccgagcgc
     5281 gatgtgggcg tcggcgtgct tgggcgcgag cgcgatcgtc gtctccagtg cgttcttgac
     5341 cttggcgccc aggccttgag tcagcgcctt ggccacgctg atgccctggc tgtagcggcc
     5401 cagcgcgtag gcctgccagt agtgcgcgtt ggccagcttc gggttggcct tggcctgcgc
     5461 ctccgcgcgc tcggccactt ccatgaacat cgcgagcttg gtcttctcgc tggattcgag
     5521 gtagttcgcg tagatggact gcgccttgtt ggcgacgacg atgccgccgt cgcccgcctt
     5581 caggcccgcg tcgtacgcct tcctgaaatc gccggcgtgg aacgcggccc aggccgcgag
     5641 caccgcgtcg tccttgggga agggctcggc gtcgcccttg tgcaggcggg cccagtgctt
     5701 cttcagggcg gcggcgtcgt acgtgtagtc ggcggcgtcg taggggaagg cggtccattt
     5761 ggccatggcg gggtctcttt cgtggtctcg aagtggaagg atcttgtcag gacttgtagg
     5821 cgcccgccag ccgctgcagg tgctcgcgcg cggccggctc caggtagggc gtgagcagcg
     5881 acaggacgtg ccacgcgccg cgcgccagcg tcgcgccggc ctggtcgggc tcaagcgcct
     5941 tgcgcggctc gcgcacgtat tcgtagctga gccagtaggt gagcacgacc accatgctct
     6001 gggccagcgc gtcgacctcg cgcgggtcga tcgccaggtg gccggcctcg gacatcccgt
     6061 tgaccagccc gcggatggcc tgggtcttgt cttcgagcac ctcctgcaac tgggtctcga
     6121 tgtgc
//
LOCUS       DATCRO010000807         9587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_661788, whole genome shotgun sequence.
ACCESSION   DATCRO010000807 DATCRO010000000
VERSION     DATCRO010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661788"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1195
                     /locus_tag="VIN75_22010"
     CDS             17..1195
                     /locus_tag="VIN75_22010"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY8976906.1"
                     /translation="MSDSIVIVSAARTPIGGMLGDFAALSAWELGAVAIKAAVERAGV
                     PGDAVDEALIGNCLMAGQGQAPARQAVRRAGLPDSAGAVTMSKMCGSGMRTMMFAHDM
                     LLAGSADVMVAGGMESMTNAPHLMFARKGVRYGAAQMFDHMALDGLEDAYDRGRAMGV
                     FAEQCVAKYGFTREAQDQFAISSTTRAKTANEDGSFDWEIAPVTLQGKAGDVLVKHDE
                     QPFKAKLDKIPSLKPAFKKDGTITAANASSISDGAAALVLMRESEAVKRGARPIARIA
                     AHAVHAQAPEWFSTAPAGAIAKVLKKTGWSAQSVDLWEVNEAFAAVTMAAMKEFDLSH
                     DIVNVHGGACALGHPIGASGARIVVTLLGALRKQGKKRGVAALCIGGGEATALAVELL
                     "
     gene            1346..1822
                     /locus_tag="VIN75_22015"
     CDS             1346..1822
                     /locus_tag="VIN75_22015"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8976907.1"
                     /translation="MTDASTIIAEEASIAALVRVGECIDHAARYTRDEIARFAALTHD
                     RNPVHHDREAARRAGLRDVIASGQHTSALMSGLAATHFSRADDGVARSMLCLHFNFAY
                     REPIFADSEVALRWVVSSTEWNASLDGVIAQLEGKATVGDAAAVIARGTVLVRRAQ"
     gene            1845..2366
                     /locus_tag="VIN75_22020"
     CDS             1845..2366
                     /locus_tag="VIN75_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976908.1"
                     /translation="MNRWLSHPRVLQVIAVGVFACIALFQLPSSPAASAHFTALAGAP
                     PFDERPAGYTLSGVLAAFHALGAQGLAGYEAYRAVDIVFPWLLCALVASVMLRIDAAR
                     ATMWPWLAAVADTIENALQYEILRTRDDLSPELVQWASLATQVKFVMYALMLLFLLAV
                     GARALWRQRRAAR"
     gene            2390..3091
                     /locus_tag="VIN75_22025"
     CDS             2390..3091
                     /locus_tag="VIN75_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8976909.1"
                     /translation="MSSSAARNVLIIGASRGLGLEFVRQYRADGARVTATARSDDGLA
                     AIAALGAKALKLDVADAASASGLAWQIDGEAFDTVFHVAGVYGPRSTGLEPPTAAEFD
                     AVMHANVLGVMRMLPQVADALAPPVGGRPVARIGILSSRMGSIGLRASAAGWLYRASK
                     AAANSVMKDASIALADRALCVAFHPGWVRTDMGGAGADIDAAESIAGMRRALAALEPS
                     QSGSFLNYDGTPLAW"
     gene            3245..4372
                     /locus_tag="VIN75_22030"
     CDS             3245..4372
                     /locus_tag="VIN75_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8976910.1"
                     /translation="MHLSEDHRAVQDAVRAYVRDRIAPNAAAWDRNCHFPREELRGLA
                     ELGCYGIAVPTEWDGAGLDYLSVSLILEEIAAGCGATSTIISVTNCPVNTIMMAFASD
                     EQKERFLKPLVRGELLGAFCLTEPHVGSEAGGLRTTATRDGDEYVLNGVKQFITSGKN
                     GDIAIVMAVTDKAAGKKGISAFLVPTNTPGYVVARIEEKMGQHASDTAQILFENCRIP
                     AANLIGEEGQGLKIALSGLEGGRIGIASQSVGMARAAFEAAVHYAKERVAFAKPIFEH
                     QAIQFKLADMATQIEAARQLIHHAASLKDAGQPCLKEAAMAKLFASEMAERVCSAAIQ
                     VFGGYGYTTDFPVERIYRDVRVCQIYEGTSEVQKILIGRGI"
     gene            4563..5186
                     /locus_tag="VIN75_22035"
     CDS             4563..5186
                     /locus_tag="VIN75_22035"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEY8976911.1"
                     /translation="MKQVLTEKSGIQPMPSSRRVGAWLQIDGSLSARIASVSGKLTVR
                     VLRQGPVRLQPAEAERLGCPAGAGAHGREVILLADGVPVVYARSVLQAVHARGTWKAI
                     RGLGNRALADLLFGLPPAHRSRFEFARFAPGSGIAASVHRRWREATGTDWGRREVWAR
                     CSVFTRRGAPLLVTECFAPTIAAFDAPQRARAVPWRTPGRRPTPGQP"
     gene            5446..7515
                     /locus_tag="VIN75_22040"
     CDS             5446..7515
                     /locus_tag="VIN75_22040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY8976912.1"
                     /translation="MTPFRPATLVVALSLAFATQVSLGADAAASAAAPVASAPPVTEA
                     GLDKSGMDSSVRPQDDLFLAMNGTWVKKTEIPADKSSWGTFYQLRELSDQRVKDIIEE
                     MASKPQAAGTVNAKIGDFYKSYMDTAAIDAAGTKPLDEFKAQLAAVKNKNDLVLLMGR
                     WVSFVDVPLNMGIGPDSKDPTIYSAGTYQGGLGLGDRDYYLKNDPRFVKNRADYVKYV
                     QTLLTLDGDKNAVMHAKAVMALEMKMAKVQWSQEANRDPVKTYNPMSVKDISAKAPNI
                     DWKAYFAATDLVDPPFVSITQPSYAWELAKLVKAQPIDVWKTYMRVRLLDAMANKLPA
                     GFREASYEFHDVALTGLKQDKPRWQRGVAAVNGALGEAIGQVYVSKYFPPAYKARMVE
                     LVDNLLKTYSTSIDGLTWMSAATKKEAHAKLAKYGVKIGYPDVWRDYSALEVKPGDAI
                     GNSVRAAQFDYHRQAVRNGKPVDRTEWGMTPQTVNAYYDPNKNEIVFPAAILQPPFFD
                     MKADDAVNYGAIGAVIGHEISHGFDDQGSQYDGDGKLRNWWTPEDRKAFEAVTSKLDA
                     QYSAYEPLPGVHLNGKLTMGENIADLSGLQIAYKAWQLSLGGKPAATVDGLSGAQRFY
                     YGFAQVWRSKVREQRTMQLVVVDPHSPAQFRADGAAINSDGYHEAFGTKPGDKMWKAP
                     ADRLRLW"
     gene            7629..>9587
                     /locus_tag="VIN75_22045"
     CDS             7629..>9587
                     /locus_tag="VIN75_22045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY8976913.1"
                     /translation="MTIFRPATLAVALSLAFAAPFTVAAGTSTVAAPTDSGAAVSLAS
                     GLDKAGMDAAARPQDSLFFSMNGTWLKNTPIPADKSDYGTFTQLYDLSNERVKGIIEK
                     LGTTPQAPGTVNAKIGDFYKAYMDTAAIDAAGMAPLKPYLDRIDAVKTRDDLVKLMGE
                     WAPFVDLPLGLGVGVDPKDPTIYSAGARQGGLGIGTRDYYLKKDARFAKARAAYLLYM
                     QALLAAQGSHDAKGEANAVMGFETRLAQAQWTQEANRDPVKTYNPMTLKALAAKAPQV
                     DWKAYFAAGQITDPPFVSIAQPSYAWAVAKMVKSEPLDVWKAYLRVHLMDALAHELPG
                     DVRTARFQFRGVAMNGTTTDRPRWERAVDTINDALGEAIGQIYVSEYFPPAYKARMVQ
                     LVDNLMKAYSASIDNLTWMSPATKVQAHAKLAKYGIKIGYPDKWRDYSALDVKAGDAL
                     GNAVRAAQFEYHRQAVRNGKPVDRTEWGMTPQTVNAYYDPTKNEIVFPAAILQPPFFD
                     MKADDAANYGAIGAVIGHEISHGFDDEGSQFDGDGKLRNWWTASDRKAFEAITGKLDA
                     QYSAYEPLPGVHVKGKLTMGENIADLSGLQIAYKAWQISLNGQPAPVIDGVSGAQRFY
                     YGFAQVWREKDRDEAMLQLVTSNPHSPPQ"
BASE COUNT         1550 a         3447 c         3261 g         1329 t
ORIGIN      
        1 tttcaggagc attgccatgt ccgattccat cgtcatcgtt tccgccgccc gcacgcccat
       61 cggcggcatg ctgggcgact tcgccgcgct gtccgcgtgg gagctgggcg ccgtcgccat
      121 caaggccgcg gtcgaacgcg ccggcgtgcc cggcgacgcc gtcgacgagg ccctgatcgg
      181 caactgcctc atggccggcc agggccaggc gcccgcgcgc caggccgtgc gccgcgccgg
      241 cctgcccgat tcggccggcg ccgtcaccat gagcaagatg tgcggctcgg gcatgcgcac
      301 gatgatgttc gcgcacgaca tgctgctggc cggcagcgcc gacgtcatgg tggccggcgg
      361 catggagagc atgaccaacg cgccgcacct gatgttcgcg cggaagggcg tgcgctacgg
      421 cgccgcgcag atgttcgacc acatggccct cgacggcctg gaagacgcct acgaccgcgg
      481 ccgcgcgatg ggcgtgttcg ccgagcagtg cgtggccaag tacggcttca cgcgcgaggc
      541 gcaggaccag ttcgcgatct cgtcgaccac gcgcgccaag accgccaacg aggacggctc
      601 gttcgactgg gagatcgcgc cggtcacgct gcagggcaag gccggcgacg tcctcgtcaa
      661 gcacgacgag cagccgttca aggccaagct cgacaagatc ccgtcgctca agccggcgtt
      721 caagaaggac ggcaccatca cggccgccaa cgcgtccagc atctcggacg gcgcggcggc
      781 gctggtgctg atgcgcgagt cggaggccgt caagcgcggc gccaggccga tcgcgcgcat
      841 cgccgcgcac gccgtgcacg cgcaggcgcc cgagtggttc tccactgccc cggccggcgc
      901 catcgccaag gtgctcaaga agacgggctg gtccgcgcaa tcggtggacc tgtgggaagt
      961 caacgaggcg ttcgccgcgg tgacgatggc cgcgatgaag gagttcgacc tgtcgcacga
     1021 catcgtcaac gtgcacggcg gcgcctgcgc cctgggccac ccgatcggcg cctcgggcgc
     1081 gcgcatcgtc gtcacgctgc tgggcgcgct gcgcaagcag ggcaagaagc gcggcgtcgc
     1141 ggcgctgtgc atcggcggcg gcgaggcgac ggcgctggcg gtcgaactgc tgtaagcacc
     1201 ccccgagctt gatacgcggc tgtaaccggc caccccgctc gcgtgcggca cactgcgtgc
     1261 ctccatgacg agccgcgccg tcgtccaccc tccgggacgg cggcgcaagc cgtccagacc
     1321 cgatcaagct cacgcctcgc ctcccatgac cgacgcctcc acgatcattg ccgaagaagc
     1381 cagcatcgcc gcgctcgtgc gcgtgggcga atgcatcgac cacgccgccc gctacacgcg
     1441 cgacgagatc gcccgcttcg ccgcgctcac gcacgaccgc aacccggtgc accacgaccg
     1501 cgaggccgcg cggcgcgcgg gcctgcgcga cgtgatcgcc agcggccagc acacctcggc
     1561 gctgatgagc ggcctggccg ccacgcactt ctcgcgcgcc gacgacggcg tcgcgcgctc
     1621 gatgctgtgc ctgcacttca acttcgccta ccgcgagccg atcttcgcgg acagcgaggt
     1681 cgcgctgcgc tgggtcgtct ccagcaccga gtggaacgcc agcctcgacg gcgtgatcgc
     1741 gcagctcgaa ggcaaggcga cggtgggcga cgcggcggcg gtcatcgcgc gcggcaccgt
     1801 gctggtgcgg cgcgcccaat gaccgtggac gggcgcgccc cgcgatgaac cgctggctgt
     1861 cgcatccgcg cgtgctgcag gtgatcgccg tcggcgtgtt cgcctgcatc gcgctgttcc
     1921 agctgccttc cagtccggcc gcgagcgcgc atttcacggc gctggccggc gccccgccgt
     1981 tcgacgagcg gcccgccggc tacacgctgt cgggcgtgct cgccgcgttc catgcgctgg
     2041 gcgcgcaggg gctggccggc tacgaggcct accgcgcggt cgacatcgtc ttcccgtggc
     2101 tgctgtgtgc gctggtggct tcggtgatgc tgcgcatcga cgccgcgcgc gccacgatgt
     2161 ggccgtggct cgcggcggtc gccgacacga tcgagaatgc gctgcagtac gagatcctgc
     2221 gcacccgcga cgacctgtcg cccgagctgg tgcaatgggc cagcctcgcc acgcaggtca
     2281 agttcgtgat gtatgcgctg atgctcctgt tcctgctggc cgtcggcgcg cgcgcgctgt
     2341 ggcgccagcg tcgcgcggcg cgttgagcgt cgccttcgaa agaccatcca tgtcttcctc
     2401 cgctgcccgc aacgtcctga tcatcggcgc ctcgcgcggc ctgggcctgg agttcgtgcg
     2461 ccagtaccgc gccgacggcg cccgcgtcac cgccaccgcg cgttcggacg acggcctggc
     2521 ggccatcgcc gcgctcggcg ccaaggcgct gaagctggac gtggccgacg ccgccagcgc
     2581 ctcgggcctg gcctggcaga tcgacggcga ggcgttcgac accgtcttcc atgtcgcggg
     2641 cgtctacggc ccgcgcagca cggggctcga gccgccgacg gcggccgaat tcgatgccgt
     2701 catgcacgcc aacgtgctgg gcgtgatgcg catgctgccg caggtggccg acgcgctggc
     2761 gccgccggtg ggcggccggc ccgtggcgcg catcggcatc ctgtcgtcgc gcatgggctc
     2821 gatcgggctg cgcgccagcg ccgccggctg gctgtaccgc gcctccaagg ccgcggccaa
     2881 ctcggtgatg aaggatgcct cgatcgcgct ggccgaccgc gcgctgtgcg tcgcgttcca
     2941 cccgggctgg gtgcgcaccg acatgggcgg cgccggcgcc gacatcgacg ccgccgaatc
     3001 catcgccggc atgcggcgcg cgctcgcggc gctcgagccg tcgcaaagcg gctccttcct
     3061 caactacgac ggcacgccgc tggcctggta atccctttgc gcgccgactt cgcctccgcg
     3121 gggtcttgca tcctgcgcgc caagcacatc ggccgtcgat ggatggcagg acgcgcaaaa
     3181 tgcaggacct gaccccgatg tggcacctcg gcgtgtcgac agactttcga cccaagagac
     3241 aaccatgcac ctctccgaag accaccgcgc cgtccaggac gccgtccgcg cctacgtgcg
     3301 cgatcgcatc gcgcccaacg ccgcggcgtg ggacaggaac tgccatttcc cgcgcgagga
     3361 actgcgcggc ctggccgagc tcggctgcta cggcatcgcg gtgcccaccg agtgggacgg
     3421 cgcggggctg gactacctgt ccgtctcgct gatcctcgag gagatcgcgg ccggctgcgg
     3481 cgccacgtcc accatcatca gcgtcaccaa ctgcccggtg aacacgatca tgatggcgtt
     3541 cgcgagcgac gagcagaagg agcgcttcct caagccgctg gtgcgcggcg agctgctggg
     3601 cgcgttctgc ctgaccgagc cgcacgtcgg ttcggaggcc ggtggactgc gcacgacggc
     3661 cacgcgcgat ggcgacgagt acgtgctcaa cggcgtcaag cagttcatca ccagcggcaa
     3721 gaacggcgac atcgcgatcg tgatggccgt caccgacaag gccgcgggca agaagggcat
     3781 cagcgccttc ctcgtgccca cgaacacgcc cggctacgtg gtggcgcgca tcgaggagaa
     3841 gatgggccag cacgccagcg acacggcgca gatcctcttc gagaactgcc gcatccccgc
     3901 ggccaacctg atcggcgagg aaggccaggg cctgaagatc gcgctgtccg ggctcgaggg
     3961 cgggcgcatc ggcatcgcgt cgcagtcggt gggcatggcg cgcgcggcgt tcgaggccgc
     4021 cgtgcactac gccaaggaac gcgtggcctt cgccaagccc atcttcgagc accaggcgat
     4081 ccagttcaag ctggccgaca tggccacgca gatcgaggcc gcgcgccagc tgatccacca
     4141 cgcggcgagc ctcaaggacg ccggccagcc ctgcctgaag gaggcggcga tggccaagct
     4201 gtttgccagc gagatggccg agcgcgtgtg ctccgcggcc atccaggtgt tcggcggcta
     4261 cggctacacc accgacttcc ccgtcgagcg catctaccgc gacgtgcgcg tgtgccagat
     4321 ctacgaaggc acgtcggagg tccagaagat cctgatcgga cgcgggatct gacggccccc
     4381 acgctcctcc agccgggaag catccggctt gcaggccgga tgccgttccg gcggggcctt
     4441 gctgcgcaag tcctttgccg cctccactcc cccgagggga gctcggttcg gctccggtgc
     4501 gaccctcgct tcccgagagc ttggctgggc gtgcggccgg cacatcctcg atacgactcc
     4561 gcatgaagca ggtattgacc gagaaaagcg gcatccagcc gatgccgtcc tcgcgccgcg
     4621 tgggcgcgtg gctgcagatc gacggctcgc tgagcgcgcg catcgcgtcg gtcagcggca
     4681 agctcacggt gcgcgtgctg cgccagggcc cggtgcgcct gcagccggcc gaggccgagc
     4741 ggctgggctg ccccgcgggc gccggtgcgc acggccgcga ggtgatcctg ctggccgacg
     4801 gcgtgccggt ggtgtacgcg cgttccgtgc tgcaggccgt gcatgcgcgc ggcacgtgga
     4861 aggcgatccg cggactgggc aatcgcgcgc tggctgatct gctgttcggc ctgccgcccg
     4921 cgcatcgctc gcggttcgag ttcgcgcgct tcgcgccggg ttccggcatc gccgccagcg
     4981 tgcaccggcg ctggcgcgag gccaccggca ccgactgggg ccggcgcgag gtgtgggcgc
     5041 gctgctcggt gttcacgcgc cgcggcgcgc cgctgctggt caccgagtgc ttcgcgccga
     5101 cgatcgccgc gttcgacgcg ccgcagcgcg cccgcgcggt gccgtggcgg acgccgggcc
     5161 gccggccgac tccgggtcaa ccctgagggg tgcggcgggg ccgtcgtatt tcgaggccat
     5221 tgccctgcag ttcgtcgcaa agcgcgggcg cggcgcggcg gtcaggggaa ccattggcat
     5281 ggtgaggcga tccacgtcgc tacactgatc cgccgctgcg cctgctgccc gacagcgcgt
     5341 gcacgcaacc gccacatcag gtggccccgg ctcctcgcga ccgcgcccca cgaaggcgcc
     5401 tgcagcgatc ggccgttcac tgccaacaac atccgaggac gtgccttgac tcccttccgc
     5461 cccgccacgc tcgtggtcgc cctgtcgctg gccttcgcca cgcaagtctc cctcggcgcc
     5521 gacgcggccg cctccgccgc ggcacccgtc gccagcgcgc ccccggtcac cgaagcgggc
     5581 ctggacaagt cgggcatgga ttcgtcggtg cgtccgcagg acgacctgtt cctcgccatg
     5641 aacggcacct gggtcaagaa gacggagatc ccggcggaca agtcgtcctg gggcacgttc
     5701 taccagctgc gcgagctgtc cgaccagcgc gtcaaggaca tcatcgagga gatggcctcc
     5761 aagccgcagg cggcgggcac ggtcaacgcc aagatcggcg acttctacaa gagctacatg
     5821 gacaccgcgg ccatcgacgc cgccggcacc aagccgctcg acgagttcaa ggcgcagctg
     5881 gcggccgtca agaacaagaa cgacctggtc ctgctgatgg gccgctgggt cagcttcgtg
     5941 gacgtgccgc tgaacatggg catcggcccc gattcgaagg acccgacgat ctactcggcc
     6001 ggcacctacc agggcggcct gggcctgggc gaccgcgact actacctgaa gaacgacccg
     6061 cgcttcgtca agaatcgcgc cgactacgtc aagtacgtgc agacgctgct gacgctggac
     6121 ggcgacaaga acgccgtcat gcacgccaag gcggtgatgg cgctcgagat gaagatggcc
     6181 aaggtgcagt ggtcgcagga agccaaccgc gacccggtca agacgtacaa cccgatgtcg
     6241 gtcaaggaca tctcggccaa ggcgcccaac atcgactgga aggcgtactt cgccgccacc
     6301 gacctggtcg acccgccgtt cgtctcgatc acccagccca gctacgcctg ggagctcgcc
     6361 aagctcgtga aggcccagcc gatcgacgtc tggaagacct acatgcgcgt gcgcctgctc
     6421 gacgcgatgg ccaacaagct gccggccggc ttccgcgagg cctcgtacga attccacgac
     6481 gtggcgctga ccggcctgaa gcaggacaag ccgcgctggc agcgcggcgt ggccgcggtc
     6541 aacggcgcgc tgggcgaggc gatcggccag gtctacgtgt ccaagtactt cccgccggcg
     6601 tacaaggccc gcatggtcga gctcgtggac aacctgctca agacctactc gacgtccatc
     6661 gacggcctga cctggatgtc ggccgccacc aagaaggagg cgcatgccaa gctggccaag
     6721 tacggcgtga agatcggcta tccggacgtc tggcgcgact attcggcgct ggaggtcaag
     6781 ccgggcgacg ccatcggcaa cagcgtgcgc gccgcgcagt tcgactacca ccgccaggcc
     6841 gtgcgcaatg gaaagccggt cgaccgcacc gagtggggca tgacgccgca gacggtcaac
     6901 gcctactacg acccgaacaa gaacgagatc gtcttcccgg ccgccatcct gcagccgccg
     6961 ttcttcgaca tgaaggccga cgacgcggtc aactacggcg ccatcggcgc ggtcatcggc
     7021 cacgagatca gccacggctt cgacgaccag ggcagccagt acgacggcga cggcaagctg
     7081 cgcaactggt ggacgccgga agaccgcaag gccttcgagg cggtcaccag caagctcgat
     7141 gcgcagtact cggcctacga gccgctgccc ggcgtgcacc tgaacggcaa gctcacgatg
     7201 ggcgagaaca tcgccgacct gtcgggcctg cagatcgcct acaaggcctg gcagctgtcg
     7261 ctgggcggca agcccgcggc caccgtcgac ggcctgagcg gcgcgcagcg cttctactac
     7321 ggcttcgcgc aggtgtggcg ctccaaggtg cgcgagcagc gcacgatgca actggtcgtc
     7381 gtcgacccgc actcgccggc gcagttccgc gcggacggcg ccgcgatcaa cagcgacggc
     7441 taccacgagg cgttcggcac caagccgggc gacaagatgt ggaaggcccc cgcggaccgg
     7501 ctgcgtctct ggtaatcggt atcggggcgg gcgcgtgagc gaccgccctt ttcgttgaaa
     7561 cgatcgctcc gcgatgcggc ggcgggcccg actgcccgcg cacgacaaca ctcgacgagg
     7621 aaacgagcat gaccatcttc cgtcccgcga cgctcgccgt cgccctctcg ctggccttcg
     7681 cggccccgtt caccgtcgcc gccggcacca gcacggtcgc cgcgccgacc gacagcggcg
     7741 ccgccgtgtc gctggcctcc ggcctcgaca aggccggcat ggacgccgcc gcgcgtccgc
     7801 aggacagcct gttcttctcg atgaacggca cctggctgaa gaacaccccg atccccgcgg
     7861 acaagtccga ctacggcacg ttcacgcagc tgtacgacct gtccaacgag cgcgtgaagg
     7921 gcatcatcga gaagctgggc acgacgccgc aggcgccggg caccgtcaac gccaagatcg
     7981 gcgacttcta caaggcctac atggacaccg cggccatcga cgccgccggc atggcgccgc
     8041 tcaagccgta cctggaccgc atcgacgcgg tcaagacgcg cgacgacctc gtcaagctga
     8101 tgggcgagtg ggccccgttc gtcgacctgc cgctgggcct gggcgtgggc gtcgacccga
     8161 aggatccgac gatctattcg gccggcgcca ggcagggcgg cctgggcatc ggcacgcgcg
     8221 actactacct caagaaggac gcgcgctttg ccaaggcgcg cgcggcctac ctcctgtaca
     8281 tgcaggcgct gctggcggcg cagggcagcc acgacgccaa gggcgaggcc aacgccgtca
     8341 tgggcttcga gacgcgactg gcccaggcgc agtggacgca ggaagccaac cgcgacccgg
     8401 tcaagacgta caacccgatg acgctcaagg cgctggccgc caaggcgccg caggtcgact
     8461 ggaaggcgta cttcgccgcc ggccagatca ccgatccgcc gttcgtctcc atcgcgcagc
     8521 ccagctatgc gtgggcggtg gcgaagatgg tcaagtccga gccgctggac gtctggaagg
     8581 cctacctgcg cgtgcacctg atggacgcgc tggcgcacga gctgcccggc gacgtgcgca
     8641 ccgcgcgctt ccagttccgc ggcgtggcga tgaacggcac caccaccgat cgtccgcgct
     8701 gggaacgcgc cgtcgacacg atcaacgacg cgctgggcga ggccatcggc cagatctacg
     8761 tgtccgagta cttcccgccg gcctacaagg cccgcatggt gcaactggtc gacaacctga
     8821 tgaaggccta ctccgcgtcg atcgacaatc tcacgtggat gagcccggcc accaaggtgc
     8881 aggcgcatgc caagctggcc aagtacggca tcaagatcgg ctatccggac aagtggcgcg
     8941 actactcggc gctcgacgtc aaggcgggcg acgcgctcgg caacgccgtg cgcgccgcgc
     9001 agttcgagta ccaccgccag gccgtgcgca acggcaagcc ggtcgaccgc accgagtggg
     9061 gcatgacgcc gcagacggtc aacgcctact acgacccgac caagaacgag atcgtgttcc
     9121 cggccgccat cctgcagccg ccgttcttcg acatgaaggc cgacgacgca gccaactacg
     9181 gcgcgatcgg cgcggtcatc ggccacgaga tcagccacgg cttcgacgac gagggcagcc
     9241 agttcgacgg cgacggtaag ctgcgcaact ggtggacggc gtccgaccgc aaggccttcg
     9301 aggcgatcac cggcaagctc gacgcgcaat actcggccta cgagccgctg cccggcgtgc
     9361 acgtcaaggg caagctgacg atgggcgaga acatcgccga cctgtcgggc ctgcagatcg
     9421 cgtacaaggc ctggcagatc tcgctgaacg gccagccggc gcccgtgatc gacggcgtca
     9481 gcggcgcgca gcgcttctac tacggcttcg cgcaggtctg gcgcgagaag gatcgcgacg
     9541 aggcgatgct gcagctggtc accagcaacc cgcactcgcc cccgcag
//
LOCUS       DATCRO010000808         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_953705, whole genome shotgun sequence.
ACCESSION   DATCRO010000808 DATCRO010000000
VERSION     DATCRO010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953705"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VIN75_22050"
     CDS             complement(<1..308)
                     /locus_tag="VIN75_22050"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811889.1"
                     /note="catalyzes the formation of L-proline from
                     L-ornithine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY8976914.1"
                     /translation="MKQFDLESTARRLDFPPLIAALERQFVGGCEVPARHVHAVGDAL
                     TALVMPAWQPGRYFGLKVVNVAPGNAARGLPGLFATYQLFDATTGAPLAAIDGGELTA
                     R"
     gene            complement(305..661)
                     /locus_tag="VIN75_22055"
     CDS             complement(305..661)
                     /locus_tag="VIN75_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976915.1"
                     /translation="MRTTEPNYIAIEQLIWLQPDGRRERVVARVGLPYPVDDGTWACA
                     AELQGVDARYPDVCGASSMQALNLATALIATRLGHLIERGERLVDAEDTSCAWDDEML
                     QSIFGLHGLRAAERVR"
     gene            complement(717..3137)
                     /locus_tag="VIN75_22060"
     CDS             complement(717..3137)
                     /locus_tag="VIN75_22060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8976916.1"
                     /translation="MTYTVAVRTLCEFTAKLGDLDLRFTPSATALEGMAGHAMVASRR
                     GEDYESEVWLKGEVRGLSVRGRADGFDPGAGRLEEVKTYRGDLHAMPQNRRSLHWAQA
                     RCYGALLCAERGLTRLDVALVYYDVTSQDETLLVETCSAEELQAFLELQCDRFLAWSM
                     QEAAHRAARDAALTALPFPYAHFRTGQRELAENVYKAAILGRTLLAQAPTGIGKTVGT
                     LFPQLKAMPRQKLDKLYFLAAKTPGRALALEALRTITAPHRAQPETADVLNELPAPNE
                     LASAGESARPPRSAGPQQGGAPPRGAGCGTPQTGGAHLRVIELVAREKACEHPTKACH
                     GDSCPLAKGFYDRLPAARQAAVGATPQSAPRLDRAGLRAIALAHEVCPYYLAQEMVRW
                     ADVVVGDYNYYFDLGGMLHGLAHAEEWRVGVLVDEAHNLVPRAREMYSATLSRAALHG
                     VRARAPAMVAPALKRLARTWNEVAGRQLEPYAVHEELPQGLLTALQNVNSAIGDHSAA
                     HPEDVPPELLQFYFDTLHFTRLAESFGTHSLFDVTIADDAQRDVTLCLRNLVPASFLK
                     PRFLAARTATLFSATLTPMHFHADLLGLPPDTAWVDVASPFDAAQLRVQVTPLSTRFA
                     HRPASLAPIAGLMARQFAAAPGNYLAFFSSFDYLEQVAALFQALHPQVPVWSQARQMA
                     ESARADFLARFQAGSRGIGFAVLGGAFAEAVDLPGERLIGAFIATLGLPQVNPVNEQM
                     KARLEALFGAGHDYTYLFPGLQKVVQAAGRVIRTTTDEGVVHLIDDRFRRPDVRRLLP
                     SWWHVETA"
     gene            3495..>3819
                     /locus_tag="VIN75_22065"
     CDS             3495..>3819
                     /locus_tag="VIN75_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEY8976917.1"
                     /translation="MTHAPSADRRKRLERLALLSAAVAGTTLVSASAHAAVSCQPWNA
                     STAYVGGDTVTENGNTYKANWWTQGNDPATNNGGAGSGQPWTLTTGCSTTPPPPTPAP
                     PPPTPA"
BASE COUNT          565 a         1361 c         1358 g          535 t
ORIGIN      
        1 cgcgcggtga gctcgccgcc gtcgatggcc gcgagcggcg cgccggtggt ggcgtcgaac
       61 agctggtacg tggcgaacaa ccccggcagc ccgcgcgcgg cattgcccgg cgccacgttg
      121 accaccttga gcccgaagta gcggccgggc tgccacgccg gcatcacgag cgcggtcagc
      181 gcgtcgccga ccgcgtgcac gtgccgcgcg ggcacctcgc agcccccgac gaactggcgc
      241 tcgagcgcgg cgatcaacgg cgggaagtcg aggcggcgcg cggtcgactc gaggtcgaac
      301 tgcttcatcg cacgcgttcg gcggcgcgca ggccgtgcag gccgaagatg gactgcagca
      361 tctcgtcgtc ccacgcgcac gaggtgtcct cggcgtcgac gaggcgctcg ccgcgctcga
      421 tgaggtggcc caggcgggtc gcgatcagcg ccgtggccag gttcagcgcc tgcatcgacg
      481 aggcgccgca gacgtccggg tagcgcgcgt ccacgccctg cagttcggcc gcgcaggccc
      541 aggtgccgtc gtccaccggg tagggcagcc cgacgcgggc gacgacgcgc tcgcggcggc
      601 cgtccggctg cagccagatc agctgttcga tggcgatgta gttgggctcg gtggtgcgca
      661 tgcagggtcc ggttcgggca agtctgcccg accgcagcca ctttgaacct tcgtgattac
      721 gcggtttcga catgccacca cgacgggagc agcctgcgca cgtcaggccg ccgaaagcgg
      781 tcgtcgatga gatggacgac gccttcgtcg gtggtggtgc ggatcacgcg acccgccgcc
      841 tgcaccacct tctgcaggcc ggggaacagg taggtgtagt cgtggcccgc gccgaacagc
      901 gcctccagcc gcgccttcat ctgctcgttg accggattga cctggggcag gcccagcgtg
      961 gcgatgaagg cgccgatcag gcgctcgccg ggcaggtcga cggcctcggc gaacgcgccg
     1021 cccagcaccg cgaagccgat gccgcggctg cccgcctgga agcgcgcgag gaagtcggcg
     1081 cgcgcggact cggccatctg gcgcgcctgc gaccacaccg gcacctgcgg atgcagcgcc
     1141 tggaacagcg cggccacctg ctccaggtag tcgaagctgc tgaagaacgc gaggtagttg
     1201 ccgggcgcgg cggcgaactg gcgcgccatc aggccggcga tcggcgccag cgacgcgggc
     1261 cggtgcgcga agcgcgtgga cagcggcgtc acctgcacgc gcagctgcgc ggcgtcgaac
     1321 ggcgaggcga cgtccaccca ggccgtgtcg ggcggcaggc ccagcaggtc ggcgtggaag
     1381 tgcatcggag tcagcgtggc cgagaacagg gtcgccgtgc gcgccgccag gaagcgcggc
     1441 ttcaggaacg aggccggcac caggttgcgc aggcacagcg tgacgtcgcg ttgcgcgtcg
     1501 tcggcgatcg tcacgtcgaa cagcgagtgc gtgccgaagg attcggccag gcgcgtgaag
     1561 tgcagcgtgt cgaagtagaa ctgcagcagc tcgggcggga cgtcctccgg atgcgcggcc
     1621 gagtggtcgc cgatcgccga gttgacgttc tgcagcgccg tgagcaggcc ctgcggcagc
     1681 tcctcgtgca cggcgtaggg ctcgagctgc cggccggcga cctcgttcca cgtgcgcgcc
     1741 agccgtttca gtgccggcgc aaccatggca ggtgcgcgcg cacgaacgcc gtggagcgcg
     1801 gcgcgcgaca gcgtcgccga gtacatctcg cgggcccgcg gcaccaggtt gtgcgcctcg
     1861 tcgacgagca cgcccacgcg ccattcctcg gcgtgcgcga ggccgtgcag catgccgccc
     1921 aggtcgaagt agtagttgta gtcgcccacc acgacgtcgg cccagcgcac catctcctgc
     1981 gccaggtagt acgggcagac ctcgtgcgcg agcgcgatcg cgcgcaggcc cgcgcggtcg
     2041 agccgcggcg cggactgcgg cgtcgccccg accgccgcct ggcgcgcggc gggcagccgg
     2101 tcgtagaagc ccttggcgag cggacacgag tcgccgtggc aggccttggt cggatgctcg
     2161 caggccttct cgcgcgccac cagttcgatc acccgcagat gtgcgccccc ggtctgcgga
     2221 gtgccgcagc cagccccccg agggggcgcg ccgccttgct gcggaccggc cgagcgcggc
     2281 ggccgcgcgc tttcgcccgc cgatgcaagt tcgttcggcg ccggcaactc gttcagcacg
     2341 tcggccgtct ccggctgcgc ccggtgcggc gccgtgatcg tgcgcagcgc ctccagcgcc
     2401 agcgcgcggc cgggcgtctt ggcagcgagg aagtacagct tgtcgagctt ctggcgcggc
     2461 atcgccttga gctgcgggaa cagcgtgccc accgtcttgc cgatgcccgt gggcgcctgc
     2521 gcgagcagcg tgcggccgag gatcgcggcc ttgtacacgt tctccgccag ctcgcgctgc
     2581 ccggtgcgga agtgcgcgta tgggaacggc agcgccgtca gcgcggcgtc gcgcgcggcg
     2641 cggtgcgcgg cctcctgcat cgaccacgcg aggaagcggt cgcactgcag ctcgaggaag
     2701 gcctgcagct cctcggcgct gcaggtctcg accagcagcg tctcgtcctg cgaggtgacg
     2761 tcgtagtaga cgagcgcgac gtccagccgg gtcagcccgc gttccgcgca cagcagcgcg
     2821 ccgtagcacc tggcctgggc ccagtgcagc gagcgccggt tctgcggcat cgcgtgcagg
     2881 tcgccgcggt aggtcttgac ctcctccagc cggccggcgc ccgggtcgaa cccgtcggcg
     2941 cggccgcgca cggacaggcc gcgcacctcg cccttgagcc agacctccga ctcgtagtcc
     3001 tcgccgcggc gcgaagcgac catggcgtgg cccgccatgc cctccagcgc ggtggccgag
     3061 ggcgtgaagc gcaggtcgag atcaccgagc ttggccgtga actcgcaaag ggtgcggacg
     3121 gcgaccgtgt acgtcatgca ctgtctattt tcccatgtct gcgctcggtc atggaggctc
     3181 gcgcaacgcc gccgtggtat ggccgaatat tcgctccagc attccaatcc atcatattgg
     3241 taccaatgtg gtattgaaaa gttcggtagc gctttcatgg tcgagcgcgc tagcgcatct
     3301 cctgcgaaac cgtaccccct agttcgttgg aaacggaggg tcgttcggct caccttacat
     3361 tcatggacca aattggtgga tcgttactgg ttctgtactg gtttcaccta tccgggcaga
     3421 cccgcgcggc gatcgagact gcgcgaaccg gccaccgttt ccggcggacc ggcctacaac
     3481 gaggagagcc cgaaatgacc catgccccca gcgccgatcg gcgcaaacga ctcgaacgac
     3541 tggccctgct gtcggccgcg gtcgccggca ccaccctggt ctcggccagc gcgcatgccg
     3601 cggtcagctg ccagccctgg aacgcctcga ccgcctatgt cggcggcgat accgtcaccg
     3661 agaacggcaa cacctacaag gccaactggt ggacgcaggg caacgacccc gcgaccaaca
     3721 acggcggagc cggctccggc cagccctgga cgctgaccac cggctgctcg accaccccgc
     3781 cgccgcccac gcccgcgccc ccgccgccca ctccggcgc
//
LOCUS       DATCRO010000809         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_661801, whole genome shotgun sequence.
ACCESSION   DATCRO010000809 DATCRO010000000
VERSION     DATCRO010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661801"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..>2183
                     /locus_tag="VIN75_22070"
     CDS             161..>2183
                     /locus_tag="VIN75_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY8976918.1"
                     /translation="MKTARSRIAQRLPLLCAMAALGGCAGYQYHTDGMKLAAEGKGDA
                     ALPLLRMATQADPLNGEYRIDLLRETAQYVGDLLVHADEARRNAQPEVAADLYNKVLR
                     VEPANDRARRGLDAIERDARSARLLVDAETLLRDEHVESARTKVDAVLSSDPANPTAR
                     RLQLRIAEAAERDREAKATALAARSVMNKPVTLQFRDANLRMVFEALSRTTGLNVILD
                     RDVRSDLKTTIFVKDAAVEDTVDLILLQNQLEKRTLNANTLFVYPATAAKQKEYQDLQ
                     VRTFHIVNADVKYMQTLLKGVLKIKEVSMDERSDTLVIRDTPEAIAVAAKVIAAHDVP
                     DAEVMLEIQVLEISHDRTSNLGVQFPTSFGVATPSDANGNLTLSDLKHLRAGSLLASP
                     NPLSVGFNFQLKDTDANLLASPRIRARNKEKAKILIGDRVPIITNSVTPVASGSSVVT
                     GSVSYQDVGLKLEFEPDIYSESEVGIKITLEVSTIAKEISGPNGSLAYQIGTRNAQTS
                     LRLRDGETQILGGLIQSNEQNTADKIPGLGHLPVVGRLFGNNSGSGTKSEIVLSITPH
                     VVRPPPVLDASISEVFSGTEGSIRQRALQLDPMGGVRMPAATPAATPPAPAPTPAPPR
                     AAAPAAAPIAAPVVAPVVVAPPPAAPAVASPAPAAAATGQTPPPGTESYM"
BASE COUNT          385 a          818 c          708 g          272 t
ORIGIN      
        1 aggccttcct cgcgcgcggc gacgaactgc tggtcgtgtc gcaaggcgac atgctggaca
       61 acggcgccta ccgcatcgac gcggtgagcc cgacgcagat cgtcttcatc catgtgccga
      121 ccaacacgaa gcagatcatc agtctgtcag gaggggcctc gtgaaaacag caagatcacg
      181 catcgcgcag cgcctgccgc tgctgtgcgc catggcggcg ctcggagggt gcgccggcta
      241 tcagtaccac accgacggca tgaagctggc cgccgagggc aagggcgacg ccgcgctgcc
      301 gctgctgcgc atggcgacgc aggcggatcc gctcaacggc gaataccgga tcgacctgtt
      361 gcgcgagacg gcgcagtacg tcggcgacct gctcgtccat gcggacgaag cgcgccgcaa
      421 cgcccaaccg gaggtggccg cggacctgta caacaaggtg ctgcgcgtcg agcccgccaa
      481 cgaccgcgcg cggcgcggcc tcgacgccat cgagcgcgac gcgcgcagcg cgcggctgct
      541 cgtcgatgcc gagacgctgc tgcgcgacga gcacgtggag tccgcgcgga cgaaggtgga
      601 cgccgtcctg tcgagcgacc cggccaaccc gaccgcacgc cggctgcaac tgaggatcgc
      661 cgaggccgcc gagcgcgatc gcgaggccaa ggcgacggcg ctcgccgcgc gctcggtgat
      721 gaacaagccc gtcacgctgc agttccgcga cgcgaacctg cgcatggtct tcgaggccct
      781 ctcgcgcacc accggcctga acgtgatcct cgaccgcgac gtgcgctcgg acctgaagac
      841 gacgatcttc gtgaaggacg cggcggtgga ggacaccgtc gacctgatcc tgctgcagaa
      901 ccagctcgag aagcgcacgc tcaacgccaa cacgctgttc gtctaccctg cgaccgcggc
      961 caagcagaag gagtaccagg acctgcaggt gcgcaccttc cacatcgtca atgccgacgt
     1021 gaagtacatg cagacgctgc tcaagggcgt gctcaagatc aaggaagtgt cgatggacga
     1081 gcgcagcgac acgctcgtca tccgcgacac gccggaagcg atcgcggtcg ccgcgaaggt
     1141 gatcgcggcg cacgacgtgc ccgacgccga ggtcatgctg gagatccagg tgctggagat
     1201 ctcgcacgac cgcacctcca acctcggcgt ccagttcccg acctcgttcg gcgtcgccac
     1261 cccgtccgac gccaacggca acctgaccct gtcggacctg aagcacctgc gcgccggcag
     1321 cctgctggcg agccccaacc cgctgtcggt cggcttcaac ttccagctca aggacaccga
     1381 cgccaacctg ctcgcgagcc cgcgcatccg ggcgcgcaac aaggagaagg ccaagatcct
     1441 catcggcgac cgggtgccca tcatcacgaa ctcggtcacg ccggtggcga gcggctccag
     1501 cgtcgtgacc ggctcggtgt cctaccagga cgtgggcctc aagctcgagt tcgagccgga
     1561 catctacagc gagagcgagg tcggcatcaa gatcaccctc gaggtcagca cgatcgccaa
     1621 ggagatcagc ggccccaacg gctcgctcgc gtaccagatc ggcacccgca acgcccagac
     1681 gtccctgcgc ctgcgcgacg gcgagaccca gatcctgggc ggcctcatcc agtccaacga
     1741 gcagaacacg gccgacaaga tccccggcct ggggcacctg ccggtcgtcg gacggctgtt
     1801 cggcaacaac agcggctccg gcaccaagtc ggagatcgtg ctgtcgatca cgccgcacgt
     1861 cgtgcggccg cccccggtcc tcgatgcctc gatcagcgag gtgttctccg gcaccgaggg
     1921 cagcatccgc cagcgcgcgc tccagctcga cccgatgggg ggcgtgcgga tgcccgcggc
     1981 cacgcctgcc gcgaccccgc ccgcgcccgc acccacaccc gcgccgccgc gcgcggccgc
     2041 gccggccgcg gccccgatcg cagcgccggt cgtggcgccc gtcgtcgtcg cgccgccgcc
     2101 tgccgcgccc gccgtcgcca gcccggcgcc cgctgcggcc gccacgggcc agacaccgcc
     2161 gccgggcacc gagtcctaca tgc
//
LOCUS       DATCRO010000810         5612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1066450, whole genome shotgun sequence.
ACCESSION   DATCRO010000810 DATCRO010000000
VERSION     DATCRO010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066450"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIN75_22075"
     CDS             <1..322
                     /locus_tag="VIN75_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976919.1"
                     /translation="TAIAAGPLAQLFPGGMTGDGIVAAASHAVDRYMALANKGKIMGF
                     FDYYMHPPIRSGYLKSSIRDASLIKFMYAYYLEAYPNPSHSYQSYPAWQANNYSWSTQ
                     QPLN"
     gene            480..1583
                     /locus_tag="VIN75_22080"
     CDS             480..1583
                     /locus_tag="VIN75_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976920.1"
                     /translation="MTRPATFRSIQLASAAAVLALATSTSALAADSGNGLAGSDFDFA
                     QGDLTSSGATVIYGTLTLKDEGKVEANIWKGTGRDPIPAMAEVGRLSPTIEPEGNLLA
                     TDKGRKVITNPRAFAGSPQPLGGQWWSAGDDVCLQLEGQPVVHLDRVKLGHQDGWGAL
                     LRSSEQAGECTRSGGGKHAMARVAAPSTVAFLSQRVFPRSRGDIKIKYQRFNGIISRL
                     NGWDPRCVLSRWEKTDLHFTPGTFESVGKDVWRAVSSEPQKKGGPMQVFSYLAVPQEV
                     TPAGRLVFMDVGHDFNRNGRIDDDWGHIYAGLPIVSKAGDFGGMMMVDMSPVGIREIP
                     FKGGCMGDGGAQLTATLGLIVYVSQDLAPDFGH"
     gene            complement(1601..2350)
                     /locus_tag="VIN75_22085"
     CDS             complement(1601..2350)
                     /locus_tag="VIN75_22085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY8976921.1"
                     /translation="MSTEYPVMDISCVAFADQREAVEHLKSLIRTGAHGYTVAINAEK
                     IMRCRRDLPFRELVSNALIKVPDGAGAVLALRILDGVRSAKIDFPQSTFIAANELGAG
                     CRLGIIGASEDSHRAAVEEVRRRYPDAQIVMNRSGFTPEDQLLAELKEVRPQLCLLSM
                     GTPKQENFAQRAVAAGVPCIFVGAGGALDILSGHAVRAPKWMVDNYLEWLYRLIQNPS
                     RWRRQLVLPVFVGQLILERLRAGRHPSRAVR"
     gene            complement(2358..4211)
                     /gene="asnB"
                     /locus_tag="VIN75_22090"
     CDS             complement(2358..4211)
                     /gene="asnB"
                     /locus_tag="VIN75_22090"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY8976922.1"
                     /translation="MCGIAGIVGPQALETDVGSMIRAIVHRGPDGDSLDCGPGFAFGH
                     ARLAIIDLVTGAQPMRSTSGRWLIVFNGEIYNYRELRAQLEARYAFATHSDTEVILAA
                     VEVHGVERGLAMIDGMFAVGLYDMAERRVHLARDHFGIKPLYWANRADGALVFASEMK
                     ALHAAGVPMPVDRTAIAVQLLCRFIPAPWTGRVGVHKLRPGEHLVFDGEGRRLGESRR
                     IVPLAASTPVAAGRDRVDQVAALMSDAVQRQTVSDVPIGILLSGGIDSALVCQSAARA
                     NPGLHSYCVGYSADDRATEFAEAEETARLVGTEHRNIVVEPEDFAGAVRHSIWHLEEP
                     VATTSLVTFSLLCKAVARERKVVLTGQGADEPWAGYTRHRFEALRGRYGPAMSLAAPL
                     IRRWAGGRARLREIVAHLDDDLLRWVAYRSLFPIERVGQVFGDEILRAALERIRDALA
                     WADGQAPEAAADPFNRLLVRDAFTDLSDNLLLLSDKLSMSHGLEVRVPLLDVAYASHV
                     LRLPRDAKRTGPTFARGKALHKEVASRTLPPQIVHRPKKGFETPLQRWLAGDFGREVQ
                     DRILGPGSGLASCFDVKALLGERPALDNVSHELQQQLFSLWLTEEWTHILK"
     gene            complement(4227..5432)
                     /locus_tag="VIN75_22095"
     CDS             complement(4227..5432)
                     /locus_tag="VIN75_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976923.1"
                     /translation="MLRARLNALWLSLGAFGGELTLLLALPAIGRLYGAPAVAILSIA
                     TVGANVGKLLGSLKVDAAIANADDARVGATHAAAVAAIVATSAAALLLFSALDGLSVL
                     PAAAAAAGPVICGAFAGGAVQQASAMRLLRENRLGAFALMKAFTSLMLVAIVFGLRPG
                     SLMTAYLLANAATVLAAAAWHLRRPAPGGSGLATATRQELRRARGFIVQGAPAAALDG
                     ANMFLLSVVTVWMAGAAVAGEAAQMQRIALGPSLAASLLLSQHVWRQRFAPDDVGAWR
                     RDAGVVARGAATAAALSAAVLCGLLWTPPGRALVHSKPGDALGIVACLAPLLAQYSVS
                     ASTVYFFKRERLHLYARLQVTLLACLGAVAGLSLATSDPALRVLLLLPLAGAILLLTA
                     TLARRAVIS"
     gene            complement(5414..>5612)
                     /locus_tag="VIN75_22100"
     CDS             complement(5414..>5612)
                     /locus_tag="VIN75_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976924.1"
                     /translation="ISVAHPAGLFLRVMTVIFVISGFQMEVAFPIASLVRAAAIALVL
                     AFLEGAARWTRQAPTPCSVPA"
BASE COUNT          871 a         1999 c         2024 g          718 t
ORIGIN      
        1 caccgcgatc gcggccggtc cgcttgcaca gctgttcccg ggcgggatga cgggcgacgg
       61 catcgtcgcg gcggcgtcgc acgcggtcga ccgctacatg gcgcttgcca acaagggcaa
      121 gatcatgggg ttcttcgact actacatgca cccgcccatc cgcagcggct acctgaagtc
      181 gtcgatccgg gatgcctcgc tgatcaagtt catgtacgcc tactacctcg aggcctaccc
      241 gaacccgtcg cacagctacc agagctatcc ggcgtggcag gccaacaact acagctggag
      301 cacccagcag ccgttgaact gaacgggctt gcgccgagct gtcggcgcgt cgcagcctcc
      361 actgttcggg tacgcttgcc gccaccggtc gcaggcacgt cgcgaccgct tccggcgcgg
      421 ccacggcttt cctgtcgtcc cgccgcgccg ccaacgcgtt gacacaacaa ggaggtccga
      481 tgacccgccc cgccaccttc cgcagcatcc agctcgccag tgccgccgcc gtcctggcgc
      541 tggccacgtc gacgagcgcc ctggccgccg attccggcaa cgggctcgcc ggctccgact
      601 tcgacttcgc gcagggcgac ctcacgtcca gcggcgccac cgtcatctac ggcacgctga
      661 cgctcaagga cgagggcaag gtcgaggcga acatctggaa gggcaccggc cgcgacccca
      721 tccccgcgat ggccgaggtc ggccgcctgt cgccgacgat cgagcccgag ggcaatttgc
      781 ttgcgaccga caagggccgc aaggtgatca cgaatccgcg cgccttcgcg ggcagcccgc
      841 agccgctcgg cggccagtgg tggagcgccg gcgacgacgt ctgcctgcag ctcgagggcc
      901 agccggtggt gcacctggac cgcgtgaagc tgggccacca ggacggctgg ggcgcgctgc
      961 tgcgcagcag cgagcaggcc ggcgagtgca cccgctcggg cggcggcaag cacgcgatgg
     1021 cgcgcgtcgc ggcgccgtcg acggtggcct tcctgtcgca gcgcgtgttc ccgcgctcgc
     1081 gcggcgacat caagatcaag taccagcgct tcaacggcat catctcgcgc ctgaacggct
     1141 gggatccgcg ctgcgtgctc tcgcgctggg agaagaccga cctgcacttc acgccgggca
     1201 ccttcgagtc cgtcggcaag gacgtgtggc gcgccgtctc gtcggagccg cagaagaaag
     1261 gcgggcccat gcaggtcttc tcgtacctgg cggtgccgca ggaagtgacg ccggccggcc
     1321 gcctggtctt catggatgtc ggccacgact tcaaccgcaa cgggcgcatc gacgacgact
     1381 ggggccacat ctacgccggc ctgcccatcg tcagcaaggc gggcgacttc ggcggcatga
     1441 tgatggtgga catgtcgccc gtgggcatcc gcgagatccc gttcaagggc ggctgcatgg
     1501 gcgacggcgg cgcgcagctg acggccacgc tgggcctgat cgtctacgtc tcgcaggacc
     1561 tcgcgcccga cttcgggcac tgagaagctg agcccccgcg tcagcggacg gcgcgggacg
     1621 gatggcgccc cgcgcgcagg cgctccagga tcagctggcc gacgaacacg ggcagcacca
     1681 gctggcggcg ccagcgtgac gggttctgga tgagccggta cagccactcc aggtagttgt
     1741 cgaccatcca cttgggcgcg cgcaccgcgt ggccggagag gatgtcgagc gcgccgccgg
     1801 cgcccacgaa gatgcatggc acccccgcgg ccacggcgcg ctgcgcgaag ttctcctgct
     1861 tgggcgtgcc catcgacagc aggcacagct gcggacgcac ttccttgagt tcggccagca
     1921 gctggtcctc gggcgtgaag ccggatcggt tcatcacgat ctgcgcgtcg gggtagcggc
     1981 ggcgcacctc ctcgacggcg gcgcgatggc tgtcctcgga cgcgccgatg atgcccaggc
     2041 ggcacccggc cccgagctcg ttggcggcga tgaaggtcga ctgcgggaag tcgatcttgg
     2101 ccgagcgcac gccgtcgagg atgcgcagcg ccagcaccgc accagcgccg tcgggcacct
     2161 tgatgagcgc gttggacacc agctcgcgga acggcaggtc gcgccggcag cgcatgatct
     2221 tctcggcgtt gatggccacc gtgtagccat gggcgccggt gcggatcagg gacttcaggt
     2281 gctccacggc ctcgcgctgg tcggcgaagg cgacgcagga gatgtccatc acggggtatt
     2341 cggtgctcat ggtgtcttca cttgaggatg tgggtccact cttcggtcag ccacagcgag
     2401 aacagctgct gctggagttc gtggctgacg ttgtcgaggg ccggacgctc gcccagcagg
     2461 gccttgacgt cgaagcagga cgcgagcccg gagcccgggc ccaggatgcg atcctgcacc
     2521 tcgcggccga agtcgcccgc cagccagcgc tgcagcggcg tctcgaagcc cttcttgggc
     2581 cggtgcacaa tctgcggcgg cagcgtgcgc gacgcgacct ccttgtgcag cgccttgccg
     2641 cgcgcgaacg tcgggccggt gcgcttggca tcgcgcggca gtctcagcac gtggctcgcg
     2701 tacgccacgt ccagcagcgg cacgcgcact tccaggccgt gcgacatcga cagcttgtcc
     2761 gacagcagca gcaggttgtc cgagaggtcg gtgaacgcgt cgcgcaccag caggcggttg
     2821 aacggatcgg cggccgcctc gggcgcctgg ccgtcggccc aggcgagcgc gtcgcggatg
     2881 cgctcgagcg cggcgcgcag gatctcgtcg ccgaacacct ggccgacgcg ctcgatcggg
     2941 aacagcgagc ggtaggcgac ccagcgcagc aggtcgtcgt ccaggtgggc gacgatctcg
     3001 cgcagccgcg cacggccgcc ggcccagcgg cggatcagcg gcgcggccag cgacatcgcc
     3061 ggaccgtacc ggccacgcag cgcctcgaag cgatggcgcg tgtagccggc ccacggctcg
     3121 tcggcgccct ggcccgtcag caccaccttg cgctcgcgcg ccacggcctt gcacagcagc
     3181 gagaacgtca ccagcgacgt ggtggccacg ggttcctcga ggtgccagat ggagtggcgc
     3241 accgcgcccg cgaaatcctc gggctcgacc acgatgttgc ggtgctcggt gcccaccagg
     3301 cgcgcggtct cctcggcctc ggcgaactcc gtggcgcggt cgtcggccga ataccccacg
     3361 cagtagctgt gcagcccggg gttggcgcgc gccgccgact ggcacaccag cgcggagtcg
     3421 atgccgcccg acagcaggat gccgatcggc acgtccgaga cggtctggcg ctgcacggcg
     3481 tcggacatca gcgcggcgac ctggtcgacg cggtcgcggc ccgcggccac cggcgtggac
     3541 gcggccagcg gcacgatgcg gcgcgactcg cccaggcgcc ggccctcgcc atcgaagacc
     3601 aggtgctcgc cggggcgcag cttgtgcacg ccgacgcggc ccgtccacgg cgcggggatg
     3661 aagcggcaca gcagctgcac ggcgatggcg gtgcggtcca cgggcatggg gacgcccgcg
     3721 gcgtgcagcg ccttcatctc ggacgcgaac acgagcgcgc cgtccgcgcg gttggcccag
     3781 tacaggggct tgatgccgaa gtggtcgcgc gccaggtgca cgcggcgctc ggccatgtcg
     3841 tacaggccga cggcgaacat gccgtcgatc atggccagcc cgcgctcgac gccgtgcacc
     3901 tcgacggccg cgaggatgac ctcggtgtcg gagtgggtgg cgaacgcgta gcgcgcctcg
     3961 agctgcgcgc gcagctcgcg gtagttgtag atctcgccgt tgaagacgat cagccagcgg
     4021 cccgaggtcg agcgcatcgg ctgggcgccg gtgaccaggt cgatgatggc cagccgcgca
     4081 tggccgaacg cgaagccggg gccgcagtcg agcgagtcgc cgtccggccc gcgatggacg
     4141 atcgcccgga tcatcgatcc gacgtcggtc tccagggcct gcgggcccac gatgccagcg
     4201 ataccgcaca tggtatgtag agtctctcag gaaatgacgg cgcgccgcgc gagcgtcgcc
     4261 gtcagtagca ggatggcccc ggccagcggc agaagcaaca ggacccgcaa cgccggatcg
     4321 gacgtcgcga gcgagaggcc ggccaccgcg cccaggcacg cgagcagcgt gacctgcagg
     4381 cgcgcgtaca ggtgcagccg ctcgcgcttg aagaagtaga ccgtggatgc cgacacgctg
     4441 tactgcgcca gcagcggcgc gaggcacgcg acgatgccca gcgcatcgcc cggcttcgaa
     4501 tgcaccaggg cgcgtcccgg cggcgtccac agcaggccgc acagcacggc cgcgctgagg
     4561 gcggcggccg tggcggcgcc acgcgcgacg acgcccgcgt cgcggcgcca ggcgccgacg
     4621 tcgtcgggcg cgaagcgctg gcgccacacg tgctggctca gcagcaggct ggccgccagc
     4681 gacgggccga gcgcgatgcg ctgcatctgc gcggcttcgc cggcgacggc ggcgccggcc
     4741 atccagacgg tgacgaccga cagcaggaac atgttggcgc cgtcgagcgc cgcggcgggc
     4801 gcgccctgca cgatgaagcc gcgcgctcgg cgcagctcct ggcgcgtggc cgtcgccagt
     4861 ccggaaccgc ccggcgcggg ccgacgcagg tgccacgccg ccgcggccag caccgtggcc
     4921 gcgttggcca gcaggtaggc cgtcatcagc gacccgggcc gcagcccgaa cacgatggcc
     4981 accagcatca gcgacgtgaa ggccttcatc agcgcgaacg cgccaagccg gttctcgcgc
     5041 agcagccgca tcgccgacgc ctgctgcacc gcgccgcccg cgaacgcgcc gcagatcacg
     5101 gggccggcgg cggcggccgc ggcaggcagc acggacaggc cgtcgagtgc cgagaacagc
     5161 agcaaggcgg cggccgaggt ggccacgatg gcggcgaccg cggccgcgtg cgtcgcgccc
     5221 acgcgggcgt cgtcggcgtt ggcgatggcc gcgtcgacct tgaggctgcc cagcagcttg
     5281 cccacgttgg ccccgacggt ggcgatgctc aggatggcca ctgccggcgc gccgtacagg
     5341 cggccgatcg caggcagcgc cagcagcagc gtgagctcgc caccgaacgc ccccagggac
     5401 agccacaggg cgttcaggcg ggcacggagc atggcgtcgg ggcttgccgc gtccagcggg
     5461 cggcgccttc gaggaaggcg agcaccagcg cgatggcggc ggcgcgcacc aggctggcga
     5521 tcgggaacgc gacttccatc tggaagccgg agatcacgaa gatgacggtc atcacgcgca
     5581 ggaacagccc ggccggatgc gccaccgaga tc
//
LOCUS       DATCRO010000811        14246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_200516, whole genome shotgun sequence.
ACCESSION   DATCRO010000811 DATCRO010000000
VERSION     DATCRO010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200516"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VIN75_22105"
     CDS             <1..506
                     /locus_tag="VIN75_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976925.1"
                     /translation="RPAASPVLDRQEALLLPRLLWAASHDRSERVVAAGRLQLVTVLE
                     AGALLYEPRAHALRPLRAMDLRIPREAAHPRQGFELVYVATIARGDPAARRALAAVDA
                     AVVGARVERLCTAYGLWLKPRGPIEARLLEALLDLEPPGFVSFVQSIGWTDEHLTRPS
                     MHAPTPG"
     gene            648..1496
                     /locus_tag="VIN75_22110"
     CDS             648..1496
                     /locus_tag="VIN75_22110"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8976926.1"
                     /translation="MLEAHARILLPAPGPEYAAPAAPPAPHAIANGPCDRWLGPGELS
                     HWSAPVAAPVPCRVSRQSILLHEGARAHTFYIVQAGDFKVSRTAEDGYEQVIAFAGAG
                     DALGLDGLASGCHLGTVEALQDAIVYAVPLADLRALRAANPAFDLCLMESLGAHWACM
                     RDMAWLMGAMGADRRTARFLLQLSGTMARRGMSPRRLRLAMSRRDIAAHLGLAHESIS
                     RAMTSLAMSGYLRVYGREVEILDMEGLEQLAANTRGHASESLAAPVRQRMAGRTAAPE
                     RACPVH"
     gene            complement(1684..2955)
                     /locus_tag="VIN75_22115"
     CDS             complement(1684..2955)
                     /locus_tag="VIN75_22115"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY8976927.1"
                     /translation="MNDSSIRIEPELHAALRRSGLRLLELRGRALLPIVQGGMGVGVS
                     AHRLAGTVAACGGVGTISAVDLRRLHPDLMERTRGRGVGPEVKAAIADANLEALAREI
                     AAARRLSAGRGLLAVNVMRAVGQYAAYVRRALECGIDAVVVGAGLPLDLPELAADHPG
                     VALVPILSDARGVQLVLRKWERKRRVPDAIVLEHPALAGGHLGAATIADLQDPRFDFE
                     RCVPETLAVLRAAGLERTPVIAAGGIRSAADVHRLQDLGASAVQLGTPFAVTREADAH
                     EAFKQVLADAGDEDLVEFTSVAGLPARAVLTPWLRTYLRALPRLQENAHVKPRCTEVF
                     DCLAQCGLRDGRPGWGQFCIDHQLAAALRGDVRRGLFFRGKGALPFGREIRGVRDLMR
                     HLLLGGDPGAMDAHQDPEAAALPRQVHRRSW"
     gene            3239..3841
                     /locus_tag="VIN75_22120"
     CDS             3239..3841
                     /locus_tag="VIN75_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8976928.1"
                     /translation="MTATLKILRDEHSAMASVLHALSRLLSVTKGERAPDFPLLRDMV
                     FYVADFPERQHHRKESELLFPKLRARASWIRKLLDRLDDEHARSEFWVQSLEHALIAW
                     EVVGEPRRAAFETAMQRYAGFYLAHMALEEREIMPLALQVLTPADWEALDDALAPEPD
                     PLTGLGPEAAYRALFQRIALTVHPAAPAATQATWTQARHP"
     gene            complement(3777..4358)
                     /locus_tag="VIN75_22125"
     CDS             complement(3777..4358)
                     /locus_tag="VIN75_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY8976929.1"
                     /translation="MKASTPDAAVPARYGRVTQLLHWLTAVLVLVAFLYGPGGPESRV
                     YAPSRDFDRHLHETLGSCVLLLVLVRVGWRLFDRRPEPAPVSRWMGLAAKIVQGLLYV
                     LLFAVPLSAILGAWLEGHPLAYLGGLEVAAPFAASHDLGVTIATIHTWLGDAILWVAG
                     LHAVAALYHQFVLKDGVLASMLPAWLLARPGGR"
     gene            complement(4440..5705)
                     /locus_tag="VIN75_22130"
     CDS             complement(4440..5705)
                     /locus_tag="VIN75_22130"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEY8976930.1"
                     /translation="MTSFETPGTWTSLSAAVGIGLLVGLERERRKAGGPRRIQAGIRT
                     FTLVALLGAALMVVRVDWIVPAAILALAGLLATAYACSTAPDGGITTAAALLLTLVLG
                     ALAIPEPEIAVSIGVIATIVLAARARLHAFVRQTITDDELRDGLILAAAALVVLPFIP
                     NRYLGAFQAFNPRTTWTIVVLIMGIGVVGHVAQRLLGAAWGLAAAGFASGFVSSLATV
                     GAMGERARQAPGMLRAAVSGALMSCVATIIQLAVVVGASSVDALRAIAGPLLGAGAAG
                     TVAAIACSIWAFRDPAPAEILAGRAFSLAKALGLAATVTALTWISFLLQDRFGQAGLT
                     VGVALAGFADAHAAAASVASLVASGRLSAQDAVVPILAASSTNAVSKTVMSITSGGAR
                     FALPVVGGVALQMATAWGLAFIHVGVRSP"
     gene            complement(5732..6928)
                     /locus_tag="VIN75_22135"
     CDS             complement(5732..6928)
                     /locus_tag="VIN75_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8976931.1"
                     /translation="MKKRSWIGIGAAAVVAMALIGWAFAPRPVEVEVAAAAEGRFEAT
                     IDEDGKTRLRDRYLISAPLAGVVSRIALREGDAVQAGASVATLTPMLSPMLDERTLRA
                     QQVRVDTAQANVQRAEARIEVARVGLEQARSEMTRSEQLAVQGFVATTKLDNDRLAVE
                     AAQKELDAAVQDRHVAVHEVDEARAAVAAVTRPGQARSFVLEAPVSGRVLRVAQTSEA
                     SVPLGAALIELGDVSRMEVVAELLTTDALQARPGSFVRIERWGGEGPLERRVRLVEPE
                     AFTKVSALGVEEQRVRVLIDITSPADRWQALGDGYRVSVRIVTQAQDKALKVPVSAVF
                     PRPEGPGMAVFVLERGRARMQPVDVGARNGTDAWVRRGLKAGATVIVYPPASVRDGAR
                     ARERKV"
     gene            complement(6930..9299)
                     /locus_tag="VIN75_22140"
     CDS             complement(6930..9299)
                     /locus_tag="VIN75_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8976932.1"
                     /translation="MKSLDRKLLRDLRLMWSQALTIALVVASGIGGFVTSLSAVDSLA
                     AARDDFYVAGHFADVFASVKRAPDSLADSLRDVPGVADVQTTVQAIVRVEIPGLPDPI
                     IGQLIGLDARHPPRMNLVSVSAGQTLAAAGAHADGSIPALVSEGFAQARHLKPGDALD
                     VLMNGKRRTLRMVGTALSPEFVFAGLWGMPDMRGYGIFWIDHDTLAAACDMDGAFNQV
                     AVKLAPGASGRAVADALSRRLSGFGGRDVIVRTDQASNQMLESEIKEQRVLGTVLPVI
                     FLGVAAFLLNVVVSRLVGTQREQIAALKALGYPNGAIALHYLKLVLVIVLAGLALGLL
                     LGDQLGAMFTGLYAEFFHFPRFTHRIAAWLLATSLAVTVATAVLGTLNAILRTVRLPP
                     AEAMRPPAPGRYRRTLLERLGIRAMGPSLRMVVRNMERRPLRTLLGIGGIAAAVAITI
                     MGNFLRDAIDVVVDTQFTLSLRGDVSMWTYEPVDDAARLGLARLPGVRQVESTRFVPV
                     LLAHGHRRERSLVRGYAPRAELYRVIDMDRRQTLLEGRGLLLTDRLADKLGVAAGDTV
                     LAEVLTGEPRTLSLTVAGTVREMMGLNAYLDRETLNRLLGDGDVSTGWVLSVERGREA
                     ALLEASKAVPQVAGAFSKATMLRNMQEISARNVRIMSTILTAFAAVISVGVVYNNARI
                     ALAERGWELASLRVLGFTRAEVSALLLGEMALSIAIALPLGMLLGWLLVHGVSEALKS
                     DQFFFPVAIQARTYAWAALCVVIAGVGSALVVRRRVDGLDLVAVLKTRE"
     gene            complement(9302..10030)
                     /locus_tag="VIN75_22145"
     CDS             complement(9302..10030)
                     /locus_tag="VIN75_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8976933.1"
                     /translation="MDDATPPVRDVVFRARGISKTYRTGEVEVQALHDVDLDVLRGEF
                     VVLLGASGSGKSTLLNILGGLDVPSAGELHFHDRQLSGASDMVLTAYRREHVGFVFQF
                     YNLIPSLTVRENVALVTDIVEDPMPVDEAIGLVGLAPRRDHFPAQLSGGEQQRVAIAR
                     AIVKKPDVLLCDEPTGALDYQTGKLVLEVIARINQELGTTAVVITHNAAIAGMADRVV
                     ILADGRIQRVESNAHKRTPAELSW"
     gene            10164..11366
                     /locus_tag="VIN75_22150"
     CDS             10164..11366
                     /locus_tag="VIN75_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY8976934.1"
                     /translation="MDAGAESPDRCVLALNAGSSSLKLALFSTNGAREIARGALDLQA
                     TRLELVLSRQGRREALPLAAPVTDSLQEVVDALLQALESHHALGNLAVVAHRVVHGGR
                     RFTAAVAVDPATLEEIDALTPLAPLHQPHSVRLMRAIHALRPDLPQTASFDTAFHARQ
                     DPLPRRFALPRRLHDEGVLRYGFHGLSYDYIARTLAATEPDVAAGRVIALHLGNGASL
                     CALRAGRSVDTSMGFSTLDGVPMGTRCGALDAGVVLYLLKQRGWSVDAVEDMLYHHSG
                     LLGVSVVSGDLRKVEAAACADANAAEALALFALRCAGEVGRLASSLQGVDALVFTAGI
                     GEHDARMRAAIVERLGWLGIALDAASNGRHARRMEAAGSRVPVFVIPTDEEQVIASDA
                     LALLGTGP"
     gene            11500..13887
                     /locus_tag="VIN75_22155"
     CDS             11500..13887
                     /locus_tag="VIN75_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY8976935.1"
                     /translation="MPPPTPLAAELRDRIDAWWRAANYLSVGQIYLFDNPLLKRALEP
                     DDIKRMRLGHWGTTPGQNFIYAHLNRAIATHDLDMIYVSGPGHGGPSVVANTWLEGSY
                     SEVYPDVSQDEAGMRRLFRQFSFPGGIPSHASPETPGSIHEGGELGYSLSHAFGAVFD
                     NPSLVVACVVGDGEAETGPLATAWQSTRFLDPAHDGAVLPILHLNGYKIANPTVLARI
                     PREELVALLHGGGWEPRFVEGHEPARMHDAMAAALDDCIRAIRAIQEAARRGSAPPQA
                     RWPMVVLVSPKGWTGPRVVDGQRVEGTFHAHQVPIADPRGNPEHLRLLDEWLRSYRPH
                     ELFDDDGRLRQDIAALSPTASRRMGSNPHANGGALLKPLHLPDFRSYAVDVPQRGIHA
                     KGDTRVLGPFVRDTCVLNEANRNFRVFGPDETISNGLGAVLEKTDRQWALPTVDGDEH
                     LATSGRVMEVLSEHQCEGWLEGYLLTGRHGLFNCYEAFVHIVDSMFNQHAKWLKVTAG
                     LPWRRPIASLNILLSSHVWRQDHNGFTHQDPGFIDHVMNKKPQVVRVYLPPDANCLLS
                     VMDHCLRSRHYVNVVVAGKHPSAQWLTMDEAAAHCEDGIGIWEWAGNETEACGPDVVF
                     ACSGDVPTLETMAAVQLLREHVPELRVRVINVVDLCRLMPREEHPHGLDARRFDELFT
                     ADKPVIFAFHAYPWLIHRLTYRRTNHANIHVRGYKEEGTITTPFDMTVLNEMDRYHLA
                     IDALDRLPVQNERRLAVKRRFRAALAQHRRWIEAHGEDLPEVRDWRWSADRLPGD"
     gene            complement(13953..>14246)
                     /locus_tag="VIN75_22160"
     CDS             complement(13953..>14246)
                     /locus_tag="VIN75_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8976936.1"
                     /translation="YQRNAAATAVRPLKGVIGVTNAITIKPRAVASDIGTRIGAALSR
                     HAWREANAIQVGVKGSTVTLRGRIDSLAERNAAEGAAWSAPGVTTVVSELTIG"
BASE COUNT         2144 a         5276 c         4891 g         1935 t
ORIGIN      
        1 cccgccccgc cgcgtccccc gtcctcgaca ggcaggaggc gttgctgctg ccgcggctgc
       61 tgtgggcggc ctcccacgac cggagcgagc gcgtcgtcgc cgccggccgg ctgcagttgg
      121 tgaccgtcct ggaggccggc gcgctcctct acgagccccg cgcccacgcc ctgcgaccat
      181 tgcgcgcaat ggatctgagg atcccgcggg aggcggcgca cccgcggcaa ggcttcgagc
      241 tcgtctacgt ggccacgatc gcgcgcggcg atcccgcggc gcgcagggcg cttgccgccg
      301 tcgatgcagc ggtcgtcggt gcccgcgtcg agcggctctg caccgcctac ggcctctggc
      361 tgaagccccg cggcccgatc gaggcgcgcc tcctggaagc gctcctcgac ctggagccgc
      421 cgggcttcgt ctccttcgtc cagtccatcg gctggacgga cgaacacctc acccggccgt
      481 cgatgcacgc tcccacgccg gggtaggacg attccgaacg cgaggcggac atcgcccact
      541 cgcgatggcg ccgcatggaa cggccgtttc atcgcccgcc gactggtgga cgatccgcgc
      601 cggcgggatg ctttggtcca acgcacgacg aaacccgagg ttgtcccatg cttgaagcgc
      661 acgcccgcat cctcctgccc gcccctggac cggagtacgc cgcccccgcg gcgccgcccg
      721 ctccccacgc gatcgccaac ggcccctgcg accgctggct cggcccgggc gagctttcgc
      781 attggtccgc cccggtcgca gcgccggttc cgtgccgcgt cagccgccag tcgatcctgc
      841 ttcacgaagg cgcacgggct cacacgttct acatcgtcca ggcgggcgac ttcaaggtct
      901 cgcgcacggc ggaggacggc tacgagcagg tgatcgcctt cgcgggcgcc ggggacgcgc
      961 tggggctcga cggcctggcc tcgggatgcc acctcggcac cgtcgaggcc ttgcaggacg
     1021 ccatcgtcta cgcggtcccg ctggccgacc tgcgcgcgct gcgtgccgcc aacccggcgt
     1081 tcgacctgtg cctgatggaa tcgctcggcg cccactgggc ctgcatgcgc gacatggcct
     1141 ggctgatggg cgccatgggc gcggaccggc gcaccgcgcg cttcctgctg caactgtccg
     1201 ggaccatggc ccggcgcggc atgtcgcccc gccgcctgcg gctggccatg agccggcgcg
     1261 acatcgcggc gcacctcggg ctggcgcacg agagcatcag ccgggccatg acttcgctgg
     1321 cgatgtccgg ctacctccgg gtgtatgggc gggaggtcga gatcctcgac atggagggcc
     1381 tggagcagct tgcggccaac acgcgaggcc atgcgtcgga aagcctggcc gcacctgtcc
     1441 gccagcggat ggcggggcgc accgccgcgc ccgaacgggc ctgcccggtt cactgagacc
     1501 tggaccaggg cgaacgatgg cgaggccccc gacgccgtcc cgcgcggccc cggggcgacg
     1561 actcgcatgc gccttcgggg atcctcgcgt tgtcgggcgc gggggtcgtc ggcctgtttc
     1621 gcgcagccga tgccgtcctc gaagcttgcg tccgcgcaag gcgcgacggg cgccgtgccc
     1681 gcatcaccac gaccttcgat gcacctggcg tggaagcgcg gcggcctcag ggtcttgatg
     1741 tgcatccatg gcgcctggat cgccgcccag cagcaggtgg cgcatcaggt cgcgcacccc
     1801 gcggatctcg cggccgaacg gaagcgcgcc cttgccgcgg aagaacaggc cgcgccggac
     1861 gtcgccacgc agcgccgcgg cgagctggtg gtcgatgcag aactggcccc agcccggcct
     1921 cccgtcgcgc aggccgcatt gcgcaaggca gtcgaacact tcggtgcagc gcggcttgac
     1981 gtgcgcgttc tcctgcagcc gcggcagcgc gcgcaggtag gtccgcagcc acggggtgag
     2041 caccgcgcgc gccggcaggc ccgcgacgct ggtgaactcg accaggtcct cgtcgcccgc
     2101 gtcggccagc acctgcttga acgcctcgtg cgcgtcggcc tcgcgcgtga ctgcgaacgg
     2161 cgtgcccagt tgcaccgcgg acgctccgag atcctgcagc cggtgcacat cggccgcgct
     2221 gcggatgccg cccgcggcga tcacgggcgt gcgctcgagg ccggcggcgc gcagcacggc
     2281 cagcgtctcg ggcacgcagc gctcgaagtc gaagcgcggg tcctgcaggt ccgcgatcgt
     2341 cgccgccccc aggtggccgc cggccagcgc cggatgctcc agcacgatgg cgtcgggcac
     2401 acgccgcttg cgttcccact tgcgcaggac cagctgcacg ccgcgtgcgt cggaaaggat
     2461 cggtacgagc gcgacgccgg ggtggtcggc cgcgagttcc ggcaggtcca acggcaggcc
     2521 cgcgccgacc accacggcat cgatgccgca ctccagcgcg cggcggacat aggccgcgta
     2581 ctggccgacg gcgcgcatca cgttgaccgc cagcagcccg cggccggcgg aaagccgccg
     2641 cgcggcggcg atctcgcgcg ccagcgcctc caggttggcg tccgctatcg cggctttgac
     2701 ctcgggaccg acgccgcgtc cgcgcgtgcg ctccatcagg tcgggatgca ggcggcgcag
     2761 gtcgacggcg ctgatcgtgc cgacgccgcc gcatgcagcg acggtgcccg ccaggcgatg
     2821 tgccgacacg ccgactccca tcccgccctg cacgatgggc agcagcgcgc gaccgcgcag
     2881 ttcgaggagc ctcaggcccg atcggcgcag ggccgcatgc agttccggct cgatccggat
     2941 cgaagaatcg ttcatgccgc cgcacgttag ggtgatcgct ccgacggggc cttgatccag
     3001 gacaacgggg cggcgcgcct tccgaaagaa ggagttccgg cgcgccgggt ctcccgcagt
     3061 tcgttgagtg ccatcaacac gggcaatccg cgcggcggta acttgcgaag catcccgatg
     3121 acccaggagc gaccgtcgca agacaccccg cctggcgaga cggacgccgc aggcgcggtc
     3181 cgaccccgct cgcgcacgcg cccgacgagc gaaatccggg aacgacgacg aggcccccat
     3241 gaccgccacg ctgaagatcc tgcgcgacga gcactccgcg atggcctcgg tgctgcacgc
     3301 gctgtcccgg ctgctctccg tgacaaaggg cgaacgcgcg cccgactttc ccttgctgcg
     3361 cgacatggtg ttctacgtgg ccgacttccc cgagcgtcag caccatcgca aggagtccga
     3421 gctgttgttc ccgaagttgc gggcgcgcgc gtcctggatc cggaagctgc tcgaccgcct
     3481 ggacgacgag cacgcccgca gcgagttctg ggtccagtcg ctcgagcatg cgctgatcgc
     3541 ctgggaggtg gtcggcgagc cgcggcgcgc ggcattcgag acggccatgc agcgctacgc
     3601 cggcttctac cttgcgcaca tggcgctcga ggaacgcgag atcatgccgc tggccctgca
     3661 ggtgctgacg cccgccgact gggaagcgct cgacgacgcg ctcgcgcccg aacccgaccc
     3721 gctcaccggg ctcgggccgg aggcggccta tcgcgcgctg ttccagcgca tcgcgctcac
     3781 cgtccacccg gccgcgccag cagccacgca ggcaacatgg acgcaagcac gccatccttg
     3841 agcacgaact ggtgatacag cgccgccacg gcatgcaggc ccgcgaccca caggatggca
     3901 tcgcccagcc acgtgtggat cgtcgcgatc gtgacgccca ggtcgtgcga cgccgcgaag
     3961 ggagcggcga cctccaggcc acccagatag gccagcgggt gtccctcgag ccaggcgccc
     4021 aggatcgcgg acaggggcac ggcgaacagc agcacgtaga gcagcccctg gacgatcttg
     4081 gcggccaggc ccatccagcg gctcaccggc gccggctccg gacggcggtc gaacagccgc
     4141 cagcccacgc gcacgaggac cagcagcagc acgcacgatc cgagcgtctc gtgcaggtgg
     4201 cggtcgaagt cccgggacgg cgcgtagaca cgcgattccg gcccgcccgg gccgtagagg
     4261 aacgccacca gcaccagcac ggccgtcagc cagtgcagca gttgcgtgac gcgtccgtac
     4321 ctcgcgggca cggccgcatc aggggtcgaa gctttcatgg tggcatttcc tccaaggtga
     4381 tggccgccgg atgcccgcgc ggcctgacgg gatcgattct cgacgcgcag gctggcctgt
     4441 cagggtgaac gaacgcccac gtggatgaac gccaggcccc acgcggtcgc catctgaagc
     4501 gccacgccac cgacgacggg cagggcaaat cgcgcgccgc ccgaggtgat cgacatgacg
     4561 gtcttggaca cggcattggt gctcgacgcg gccaggatcg gcacgacggc gtcctgggcc
     4621 gacagccggc ccgatgcgac cagcgaggcg accgatgccg ccgccgcgtg cgcgtcggcg
     4681 aagcccgcga gagcgacgcc gacggtcagg cccgcctgcc cgaagcgatc ctggagcagg
     4741 aacgagatcc aggtcagcgc cgtgacggtc gcggccaggc ccagcgcctt ggcgaggctg
     4801 aacgcccggc cggccaggat ctccgccggc gccggatctc gaaacgccca gatggaacag
     4861 gcgatcgccg cgacggtgcc ggccgcgccg gcgcccagca gcgggccggc gatggcccgc
     4921 agcgcatcga cactggacgc gccgaccacc accgccagct ggatgatcgt ggcgacgcac
     4981 gacatcaacg cgccggacac cgccgcgcgc agcatgccgg gcgcttgccg cgcgcgctcg
     5041 cccatcgcgc ctaccgtcgc caggctcgac acgaagcccg aggcgaagcc ggccgcggcc
     5101 aggccccagg ccgcgcccag cagccgctgc gcgacatggc ccacgacgcc gatgcccatg
     5161 atcagcacga cgatggtcca ggtcgtccgc gggttgaagg cctgaaacgc gccgaggtag
     5221 cggttcggga tgaagggcag caccaccagc gcggccgccg cgaggatcag gccgtcgcgc
     5281 agttcgtcgt cggtgatcgt ctggcggacg aacgcgtgca ggcgggcacg cgcggccagc
     5341 acgatcgtcg cgatgacgcc gatcgagacg gcgatctccg gctcgggaat cgccagcgcg
     5401 ccgagcacga gcgtcagcag cagtgccgcc gcggtggtga tcccgccgtc cggcgcggtg
     5461 ctgcaggcgt acgccgttgc cagcaggccc gccagggcga ggatggccgc gggcacgatc
     5521 cagtcgacgc gcaccaccat caacgcagcg cccagcagcg cgacgagcgt gaaggtgcgg
     5581 atgcccgcct ggatgcggcg cgggccgccg gccttgcgac gctcgcgctc gagtccgacc
     5641 agcaggccga tgccgaccgc ggcggacagc gatgtccagg tgcctggcgt ctcgaagctg
     5701 gtcatgacgt ggatgcaggg ccgacgtccc gtcagacctt gcgttccctg gcccgcgcgc
     5761 cgtcgcgcac gctggccggc gggtacacga tcaccgtcgc gccggccttc agcccgcgcc
     5821 ggacccaggc atccgtgcca ttgcgcgcac cgacgtccac cggctgcatg cgggcgcgcc
     5881 cgcgctcgag cacgaacacg gccatgcccg gtccctcggg ccgcgggaac accgcgctca
     5941 cgggcacctt caacgccttg tcctgcgcct gcgtgacgat gcgcacgctc acccgatagc
     6001 cgtcgccgag cgcctgccag cggtcggccg gactcgtgat gtcgatgagc acgcgcacgc
     6061 gctgctcctc cacgccgagc gccgacacct tcgtgaacgc ttccggctcg accaggcgaa
     6121 cgcggcgttc cagcggcccc tcgccgcccc aacgttcgat gcgaacgaag ctgcccggcc
     6181 gggcctgcag ggcatcggtc gtcagcagtt cggcgacgac ctccatgcgc gagacgtcgc
     6241 ccagctcgat cagcgcggcg cccagcggca cgctggcctc gctggtctgc gcgacgcgca
     6301 gcacgcgtcc gctcaccggc gcctcgagca cgaagctgcg cgcctggccc gggcgcgtga
     6361 cggccgcgac ggccgcgcgc gcctcgtcca cctcatgcac cgcgacgtgg cggtcctgga
     6421 ccgcggcgtc gagttccttc tgcgccgcct ccaccgcgag gcggtcgttg tccagcttgg
     6481 tggtcgcgac gaaaccttgc acggccagct gctcgctgcg cgtcatctcg ctgcgcgcct
     6541 gctccagccc cacgcgcgcg acctcgatgc gcgcctcggc acgctgcacg ttggcctgcg
     6601 ccgtgtcgac gcgcacctgc tgcgcacgca gcgtccgctc gtcgagcatc ggcgacagca
     6661 tcggcgtgag cgtcgccacg gacgcccccg cctggacggc atcgccctcg cgcagggcga
     6721 tccggctcac gacgccggcc agcggcgccg agatcaggta gcggtcgcgc agccgcgtct
     6781 tgccgtcctc gtcgatggtg gcctcgaacc ggccctcggc cgccgccgcg acctcgacct
     6841 ccaccggccg cggcgcgaag gcccacccga tcaaggccat tgccaccacc gccgcggcgc
     6901 cgatgccgat ccacgaacgc ttcttcatct cattccctcg tcttcagcac ggcaaccagg
     6961 tccagtccat ccacgcggcg gcgcaccacc agcgcgctgc ccacgcccgc gatcacgacg
     7021 cacagcgctg cccacgcgta ggtgcgcgcc tggatcgcca cgggaaagaa gaactggtcg
     7081 gacttgagcg cctcgctgac gccgtgcacc agcagccagc ccagcagcat gcccagcggc
     7141 agcgcgatgg cgatggacag cgccatctcg ccgagcagca gcgccgacac ctcggcgcgc
     7201 gtgaagccca gcacgcgcag gctggccagt tcccagccgc gctccgcgag cgcgatgcgg
     7261 gcgttgttgt agacgacgcc gaccgagatc acggcggcga aggccgtgag gatcgtgctc
     7321 atgatccgca cgttgcgcgc gctgatctcc tgcatgttgc gcagcatcgt cgccttgctg
     7381 aacgcaccgg ccacctgggg cacggccttg gacgcctcga gcagcgccgc ctcccggccg
     7441 cgctcgaccg acagcaccca gccggtgctg acgtccccgt cgcccagcag gcggttcaag
     7501 gtctcgcgat cgaggtaggc gttcaggccc atcatctcgc gcaccgtgcc cgcgaccgtc
     7561 agcgacagcg tgcgaggctc gcccgtgagg acctcggcca gcactgtgtc gcccgccgcc
     7621 acgccgagct tgtcggccag gcggtcggtg agcagcaggc cgcgcccttc gagcaaggtc
     7681 tggcgccgat ccatgtcgat cacccgatag agctcggccc gcggcgcgta gccgcgcacc
     7741 aggctgcgtt cgcgccggtg gccgtgcgcg agcagcaccg gcacgaagcg cgtggactcg
     7801 acctgcctga cacccggcaa gcgtgccagg ccgaggcgcg ccgcatcgtc gaccggctcg
     7861 tacgtccaca tcgagacgtc gccgcgcagc gacagcgtga actgcgtgtc cacgacgacg
     7921 tcgatcgcgt cgcgcaggaa gttgcccatg atcgtgatcg ccaccgccgc cgcgatgccg
     7981 ccgatgccca gcagcgtacg caggggacgg cgctccatgt tgcgcacgac catgcgcagc
     8041 gacggaccca tcgcccggat gcccaggcgt tccagcaggg tcctgcgata gcggcccggc
     8101 gccggcgggc gcatggcctc ggccggcgga agccggacgg ttcgaagaat cgcgttcagc
     8161 gttcccagca ctgcggtcgc cacggtgacg gccaggctcg tcgccagcag ccaggccgcg
     8221 atgcgatgcg tgaatcgcgg gaagtggaag aactcggcgt acagccccgt gaacatcgcc
     8281 cccagttggt ctcccaacag gaggcccagg gccaggcccg cgagcacgat cacgagcacc
     8341 agcttcaggt agtgcagcgc gatcgccccg ttggggtagc ccagcgcctt gagcgcggcg
     8401 atctgctcgc gctgggtgcc caccagccgc gagaccacca cgttcagaag gaaggctgcc
     8461 acgccgagga agatcacggg cagcaccgtg ccgaggacgc gctgttcctt gatctcgctc
     8521 tccagcatct ggttcgacgc ctggtcggtg cggacgatga catcgcggcc gccgaagccg
     8581 gacagccgcc gggacagcgc gtcggcgacc gcgcggccgg aggcgccggg cgccagcttc
     8641 accgcgacct ggttgaacgc gccgtccatg tcgcaggccg ccgccagggt gtcgtggtcg
     8701 atccagaaga tgccgtagcc gcgcatgtcg ggcatgcccc acaggcccgc gaagacgaac
     8761 tccggcgaca gcgccgtccc gaccatgcgc agcgtccggc gcttgccgtt catcagcacg
     8821 tccagcgcgt cgcccggctt caggtgccgc gcctgcgcga agccttccga caccagcgcc
     8881 ggaatgctgc cgtcggcgtg cgccccggcc gcggcgagcg tctgcccggc gctgacgctg
     8941 accaggttca tgcgcggtgg atgccgcgca tccaggccga tcagctggcc gatgatgggg
     9001 tcgggcaggc cgggaatctc gacccgcacg atggcctgca cggtggtctg cacgtcggcg
     9061 acgcccggca cgtcgcgcag cgaatcggcc agcgaatccg gagcccgctt gacgctggcg
     9121 aagacgtccg cgaaatggcc cgcgacgtag aaatcgtcgc gcgcggctgc gagcgagtcg
     9181 accgccgaca ggctggtgac gaagccgccg atcccgctgg cgaccacgag cgcgatggtg
     9241 agcgcctggc tccacatcag gcgcaggtcg cgcagcagct tgcggtcgag cgacttcatg
     9301 gctaccagct cagctcggcg ggagtgcgtt tgtgcgcgtt ggactcgacg cgctggatgc
     9361 ggccgtcggc caggatcacc acccggtcgg ccatcccggc gatcgccgcg ttgtgcgtga
     9421 tgacgaccgc ggtggtgccc agttcctggt tgatccgggc gatgacctcc agcaccagct
     9481 tgcccgtctg gtagtcgagc gcgccggtcg gttcgtcgca caacagcacg tccggcttct
     9541 tgacgatcgc gcgtgcgatc gccacccgct gctgctcgcc gcccgacaac tgcgccggga
     9601 agtggtcgcg gcgcggcgcc aggccgacca gcccgatcgc ctcgtcgacc ggcatcgggt
     9661 cctccacgat gtcggtgacc agcgcgacat tctcccgcac ggtcaggctc gggatcaggt
     9721 tgtagaactg gaagacgaag cccacgtgct cgcgccggta ggccgtgagc accatgtccg
     9781 acgcaccgct cagctggcgg tcatggaagt gcagttcgcc cgcgctcggc acgtcgaggc
     9841 cgcccaggat gttgagcagg gtggacttgc cgctgcccga tgcgcccagg aggacgacga
     9901 actcgccgcg caggacgtcc aggtcgacgt cgtgcagcgc ctgcacctcc acctcgccgg
     9961 tgcgataggt cttggagatg ccgcgcgcgc ggaagacgac gtcgcggacg ggaggggtcg
    10021 cgtcatccat gtcgacgtga ttgtcgggat gcaggtcacg ccgccgcttg cggcagcgca
    10081 aggccgcgtc gcaacgtcgc caggcgagcg acgaagtcga ctcgcacggg tctaatattc
    10141 gacggcacaa cccagacatc gccatggatg ccggagccga atcccccgac cgatgcgtgc
    10201 tcgccctgaa cgccgggtcg tcgtcgctca agctggccct gttctcgacg aacggtgccc
    10261 gcgagatcgc gcgcggcgcc ctcgacctgc aggcgacccg cctcgagctc gtcctgtcgc
    10321 gccaagggcg acgggaagca ctgccgctgg ccgcgccggt caccgattcc ctgcaggagg
    10381 tggtggacgc gctgctgcag gcgctcgaat cccatcacgc gctcggcaac ctcgccgtgg
    10441 tggcgcatcg cgtcgtgcat ggtggccggc gattcaccgc ggccgtcgcg gtcgaccccg
    10501 cgacgctgga ggagatcgat gcgctgacgc cgctggctcc gctgcaccag ccgcacagcg
    10561 tccgcctgat gcgggctatc cacgccctgc ggcccgatct gccgcagacg gcgtcgttcg
    10621 acacggcgtt ccatgcgcgc caggatcccc tgccccgccg cttcgcgctg ccgcggcggc
    10681 tccacgacga aggcgtgctg cgctacggat tccatggcct gtcctacgac tacatcgcac
    10741 ggacgctggc cgcaaccgag ccggatgtcg cagcgggacg ggtgatcgcg ctgcaccttg
    10801 gcaacggcgc cagcctgtgc gcgctgcggg ccggccgcag cgtcgacacg agcatgggct
    10861 tctcgacgct ggacggcgtt cccatgggca cacgctgcgg cgcgctcgac gcgggcgtcg
    10921 tgctgtacct gctcaagcag cgcgggtgga gcgtcgatgc ggtcgaggac atgctctatc
    10981 accacagcgg cctgctcggc gtctcggtcg tgagtggaga cctgcgcaag gtcgaggcag
    11041 cggcctgcgc ggatgcgaac gccgccgagg ccttagccct gttcgcgctg cgctgcgcgg
    11101 gcgaggtcgg ccgtctcgcg tcatcgctgc aaggcgtcga cgccctggtg ttcaccgcgg
    11161 gcatcgggga gcacgacgcg cgcatgcggg ctgccatcgt cgagcgactg ggatggctgg
    11221 gcatcgcgct cgatgccgcg tcgaacgggc gccatgcgcg acgtatggaa gccgccggca
    11281 gccgagttcc ggtgttcgtg atccccaccg acgaggagca ggtgatcgcc agcgacgcgt
    11341 tggcgctgct cggcaccgga ccctgaagac gttctgttgc gctgcctcaa tcccggcgca
    11401 accccgcttc gacaagtggc ggcgacaacg gccaagatgg cgagtccgcg tctcgtgttc
    11461 gaacggcgcg gtccccacgc gaccagacaa ggagccgcca tgcccccgcc caccccgctc
    11521 gccgccgagc tgcgcgaccg catcgacgcc tggtggcgcg ccgccaacta cctgtcggtc
    11581 ggccagatct atctgttcga caacccgctg ctcaagcgcg ccctggagcc cgacgacatc
    11641 aagcgcatgc gcctgggcca ttgggggacg acgccgggcc agaacttcat ctacgcgcac
    11701 ctgaaccgcg cgatcgccac gcacgacctg gacatgatct acgtgagcgg cccgggccac
    11761 ggcgggccgt ccgtggtcgc caacacgtgg ctggagggca gctacagcga ggtctatcct
    11821 gacgtgtcgc aggacgaggc gggcatgcgc cgtctgttcc gccagttctc cttcccgggc
    11881 ggcatcccga gccacgcctc gccggagacg ccgggctcga tccacgaagg cggcgagctc
    11941 ggctactcgc tgtcgcatgc gttcggcgcg gtgttcgaca acccctcgct ggtcgtggcc
    12001 tgcgtcgtcg gcgacggcga ggcggagacg gggccgctcg ccacggcgtg gcagtcgacc
    12061 cgcttcctcg atcccgcgca cgacggcgcg gtgctgccga tcctgcacct gaacggctac
    12121 aagatcgcga acccgaccgt gctggcgcgc atcccgcgcg aggaactggt cgcgctgctg
    12181 cacggcggcg gctgggagcc gcgcttcgtc gaggggcacg aacctgcgcg catgcacgac
    12241 gcgatggccg cggcgctcga cgactgcatc cgcgccatcc gagcgatcca ggaggcggca
    12301 cgccggggct ccgcgccacc gcaggcgcgt tggccaatgg tcgtgctggt gtcgcccaag
    12361 ggctggacgg gcccgcgcgt cgtcgacggg cagcgcgtgg agggcacgtt tcacgcgcac
    12421 caggtgccga tcgccgaccc gcgcggcaac ccggagcacc tgcggctgct ggacgagtgg
    12481 ctgcggagct accggccgca cgaactgttc gacgacgatg ggcgccttcg ccaggacatc
    12541 gccgcgctgt cgcccacggc cagccggcgc atgggctcga acccgcacgc gaacggcggc
    12601 gcgctcctga agccgcttca cctgcccgac ttccgcagct acgcggtcga cgttccccaa
    12661 cgcggcatcc acgccaaggg cgacacccgc gtgctgggcc ccttcgtgcg cgacacctgc
    12721 gtgctcaacg aggccaatcg caacttccgc gtcttcggcc ccgacgagac gatctccaac
    12781 ggcctgggcg ccgtgctgga gaagaccgat cgccagtggg cgctgcccac cgtcgacggc
    12841 gacgagcacc tcgcgaccag tggccgcgtg atggaggtct tgtccgagca ccagtgcgag
    12901 ggctggctgg aaggctacct gctgacgggc cggcacggcc tgttcaattg ctacgaggcc
    12961 ttcgtgcaca tcgtcgactc gatgttcaac cagcacgcca agtggctcaa ggtcacggcc
    13021 gggctgccgt ggcggcgccc gatcgcctcg ctcaacatcc tcttgtcgtc gcacgtctgg
    13081 cgccaggacc acaacggctt cacccaccag gaccccggct tcatcgacca cgtcatgaac
    13141 aagaagccgc aggtcgtgcg cgtctacctg ccgcccgacg ccaactgcct gctttcggtc
    13201 atggaccatt gcctgcgcag ccggcactac gtcaacgtcg tggtcgcggg caagcatccg
    13261 tctgcgcagt ggctgaccat ggacgaggcc gccgcgcact gcgaggacgg catcggcatc
    13321 tgggaatggg ccggcaacga gaccgaggcc tgcgggccgg acgtcgtctt cgcgtgcagc
    13381 ggcgacgtcc ccacgctcga gacgatggcg gcggtgcagc tcctgcgcga gcacgtgccc
    13441 gagctgcggg tgcgcgtcat caacgtcgtc gacctctgcc gcctgatgcc gcgggaggag
    13501 catccgcacg ggctggacgc ccgtcgcttc gacgaactgt tcaccgccga caagcccgtc
    13561 atcttcgcct tccacgccta cccgtggctc atccaccggc tcacctaccg gcggacgaac
    13621 cacgccaaca tccacgtccg cggctacaag gaggaaggca ccatcacgac gccgttcgac
    13681 atgacggtgc tcaacgagat ggatcgctac cacctggcca tcgacgcgct ggaccggctg
    13741 ccggtccaga acgagcggag actcgcggtc aagcgccgct tccgtgccgc gctcgcgcag
    13801 catcgccgat ggatcgaggc gcacggcgag gacctgcccg aggtgcgaga ctggcgctgg
    13861 agcgcagatc gtctgccggg tgattgaact gctggccgac gacgaagccc gggtcacacg
    13921 acattgcgcc actgcgcccg gggtcctgtc gctcagccga tcgtcagttc gctgacgacg
    13981 gtcgtcacgc ccggggccga ccaggccgca ccttccgcgg cattgcgctc ggccagcgaa
    14041 tcgatcctgc cgcgcagcgt cacggtcgat cccttgacgc cgacctggat ggcgttcgcc
    14101 tcgcgccagg catgacggga caaggcggcc ccgatgcggg tgccgatgtc cgacgcgacc
    14161 gcacgcggct tgatggtgat ggcattggtc acgccgatca cgcctttcaa cgggcgcacc
    14221 gccgtggcgg ccgcgttgcg ctggta
//
LOCUS       DATCRO010000812         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_561028, whole genome shotgun sequence.
ACCESSION   DATCRO010000812 DATCRO010000000
VERSION     DATCRO010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561028"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VIN75_22165"
     CDS             complement(<1..685)
                     /locus_tag="VIN75_22165"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8976937.1"
                     /translation="MSITRPGLASMSPALQPIVGALLLAFGAAGAQAQSLTEVVQAAR
                     SVDATYLGARASADAAHYRYEQARAAHLPTATLQVQAGRQTSTQPTVIGVADPNDPTK
                     VAEVTATEKTSSNSAGATLNAQQTIYNRQNDLQIDQAKKNEELAQSQLAQAEQDLIVR
                     VSQAYFDVLAASDALHSVEGNSKAIGEQLASARRNFEVGNATITDTREAEARADLAHS
                     QQIAAENDLV"
     gene            complement(911..1564)
                     /locus_tag="VIN75_22170"
     CDS             complement(911..1564)
                     /locus_tag="VIN75_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY8976938.1"
                     /translation="MNIEQARFNMIEQQIRPWDVLDKGVLGLLAIVRREDFVPAAYKA
                     LAFVDTEVPLPEGESMLAPRVEARMLQDLQVQRHERVLEIGAGSGFMAALLGHRAQSV
                     ISLEVKPALARLATENLRRSGASNVTVVQADGSHGYAAEGPFDAIMLSGSVSKVPEEL
                     LKQLKPGGRLVAIEGDEPVMAAVRYTANADGTFTRTSLFDLDAPRLVGFPEPARFHF"
     gene            complement(1613..2308)
                     /locus_tag="VIN75_22175"
     CDS             complement(1613..2308)
                     /locus_tag="VIN75_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8976939.1"
                     /translation="MPPFSPDPCPFRRRRKDARPAELLEAALALFVEKGFAATRSEEV
                     ARAAGVSKGTLYLYFPSKEELLKAVIQHFMGTEIETGIQEAAAADGPVAEAMETLLVN
                     WWTRIYEGPASGVFKLMITEVRNFPEIGEFWVERVVVPGHAIVSGLIRRGIERGEFEV
                     DDVDSAVHSILMPLILECVHKHSFMACGIGKEIEIDPIAFMHAHLKLVFRGMAARRPA
                     PTPGDGRKAGQPA"
BASE COUNT          325 a          784 c          833 g          408 t
ORIGIN      
        1 cgacgagatc gttctccgcc gcgatctgct gcgagtgcgc caggtcggcg cgggcctcgg
       61 cctcgcgcgt gtcggtgatg gtggcgttgc cgacctcgaa gttgcgcctg gccgacgcga
      121 gctgctcgcc gatcgccttg ctgttgcctt cgaccgagtg cagcgcgtcg ctggcggcca
      181 gcacgtcgaa gtaggcctgc gacacgcgaa cgatcaggtc ctgttccgcc tgcgcgagct
      241 gcgactgcgc gagctcctcg ttcttcttcg cctggtcgat ctgcaggtcg ttctggcggt
      301 tgtagatcgt ctgctgcgcg ttgagcgtgg cgcccgcgct gttgctggag gtcttctcgg
      361 tggcggtcac ctcggccacc ttggtgggat cgttgggatc ggccacgccg atgaccgtcg
      421 gctgcgtgct ggtctggcgt cccgcctgca cctgcagggt ggccgtcggc aggtgcgccg
      481 cgcgcgcctg ctcgtagcgg tagtgcgccg cgtcggcgct ggcgcgtgcg cccaggtacg
      541 tggcgtcgac gctgcgcgcc gcctgcacga cctcggtcag gctctgggcc tgggcaccgg
      601 cggcgccgaa ggccaggagg agggcgccga cgatcggctg gagggccggc gacatcgagg
      661 caagaccggg acgcgtaatc gacatgcggg atccttggtt gttctggccg tttggcgttg
      721 ggacaggttt gtacggcctt gcgggccgag atgcacgttc gatgcgacga cctgcagccg
      781 acgcaccccc tgacgacgtt ttcagccgac agactgcgga ccagccgcgt tcgatcgact
      841 caggcgcagc gttcccccgg caggcccgct ggccttccgg cacgcgctgt ctctttcgcg
      901 ggtcgcggga tcagaaatgg aaccgcgcgg gctccgggaa gccgaccagg cgcggggcgt
      961 cgaggtcgaa caggctggtg cgggtgaacg tgccgtcggc attggccgtg tagcgcaccg
     1021 ccgccatcac gggctcgtcg ccctcgatcg ccaccaggcg accgcccggc ttgagttgct
     1081 tgagcagttc ttcgggcacc ttggagaccg agccggacag catgatcgcg tcgaacgggc
     1141 cttccgcggc gtagccgtgc gagccgtcgg cctgcaccac ggtgacgttg gacgcgccgc
     1201 tgcggcgcag gttctccgtg gccagcctgg ccagcgcggg cttgacctcc agcgagatca
     1261 cgctctgcgc gcggtggccc agcagggcgg ccatgaagcc cgagccggcg ccgatctcca
     1321 ggacgcgctc gtgccgctgc acctgcaggt cctgcagcat gcgggcctcg acgcgcgggg
     1381 ccagcatcga ctcgccctcg ggcagcggca cttcggtgtc gacgaaggcg agtgccttgt
     1441 aggcggccgg cacgaaatcc tcgcggcgca cgatggccag cagccccagc acgcccttgt
     1501 ccagcacgtc ccacgggcgg atctgctgtt cgatcatgtt gaagcgggct tgttcgatgt
     1561 tcatgtttct tcctcggagc gaaagggggc cttgcgggca aaccctcaaa ttctacgcgg
     1621 gctggccggc cttgcggccg tcgcccgggg tgggcgcggg ccggcgcgcc gccatgcccc
     1681 tgaagacgag cttcaggtgc gcgtgcatga aggcgatggg gtcgatctcg atctccttgc
     1741 cgatgccgca cgccatgaag gagtgcttgt gcacgcactc caggatcagc ggcatcagga
     1801 tggagtggac ggccgaatcg acgtcgtcca cctcgaattc gccgcgctcg atgccgcgcc
     1861 ggatcaggcc cgagacgatc gcgtggccgg gcacgacgac gcgctcgacc cagaactcgc
     1921 cgatctcggg gaagttgcgc acctcggtga tcatcagctt gaagacgccc gacgccgggc
     1981 cttcgtagat gcgcgtccac cagttgacca gcagcgtctc catcgcctcg gccacaggcc
     2041 cgtcggccgc ggcggcttcc tggatgcccg tctcgatctc ggtgcccatg aagtgctgga
     2101 tcaccgcctt gagcagttct tccttgctgg ggaagtaaag gtacagcgtg cccttggaca
     2161 ccccggccgc gcgggcgacc tcctcgctgc gggtggccgc gaaccccttc tcgacgaaca
     2221 gcgccagcgc ggcctcgagc aattcggccg ggcgggcgtc cttgcggcgg cgtcggaacg
     2281 ggcagggatc gggggaaaac gggggcatgt cgttaatgac tcgttggtca gtaatgtagc
     2341 agcaatcgcc
//
LOCUS       DATCRO010000813         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_492845, whole genome shotgun sequence.
ACCESSION   DATCRO010000813 DATCRO010000000
VERSION     DATCRO010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492845"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="VIN75_22180"
     CDS             complement(<1..2114)
                     /locus_tag="VIN75_22180"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HEY8976940.1"
                     /translation="MNKTLETAFRGLRGIALVMLAASILAACGGGADTASPARDDTLE
                     AANAATAQTVLHAVPPSTIAATAAGSGRRKVADVVATSLRVHYHRLNSDYSGWQIHTW
                     NAAQSPAWNGGWDASGTDDFGVYYDVPLAASSGTVGYLFHDGDTKDDNGADQSYVLVN
                     GANEIWRVEGDLTTYASNPLTSPAPDISTLRVHYIRFASDYANWGLHLWGGSGLDTSR
                     MGTISYGDWNNPTPFSAMPGYAPGGGEIVFDIPVLNPRSNPGTTSIEFLIHGMPPNIN
                     DKDGRPNNIHVDFGSLAIADQVGQVWLVEQDATVYTAQPDLRSVSTTDARAVWLNGSL
                     VKWPRVAADGPVKLYWSATAAIVAAKDQPVAGADGSITLDPYTGAVPAAAATRFKYVG
                     NGGVFQVRATDAPLLKKLLTNQVVLVQEDATGAIQNATTAQLAGALDDLYAPAASTAP
                     LGATVDHGRTTFRLWAPTARNVSLYAYLGATAGARLALPMAFDASTGIWSLTVPGEWD
                     GLYYMYGVQVFVRGTGVVRNIVTDPYSLSLSANSLRSQVTDLSSPRLKPPGWDFSRPP
                     QTVAGSTDMSIYELHVRDFSDNDPTVPAAHRGKFMAFTDVNSNGMRHLRALAQAGLTD
                     VHLMPSFDISSIPEIGCTTPSPGGAPDSTSQQATVAATASTDCYNWGYDPFHFTAPEG
                     SYATSVADGSTRVVEFRRMVQSL"
     gene            complement(2248..3279)
                     /locus_tag="VIN75_22185"
     CDS             complement(2248..3279)
                     /locus_tag="VIN75_22185"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8976941.1"
                     /translation="MRTVLDPHLVDPSGREPGLWIAVRDQRRALLFDLGELGALSPRT
                     LLRSTHAVVTHPHMDHFAGFDHWLATALGRMPGMTVWGGPGFVDKVDHKLRAYTWNVV
                     HRYAVPLVIEARSLEADGSRCGARFDSREAFARRDLPAEPARGDVLLEEPLLRLRARI
                     VDHEMPVLACAVEETAQVRVAADRIAAMGLATGAWLQALKAAVLAGAPDDDTLDVRWR
                     DAAGDHERRFRLAELRPLLLDTTPGRRIGYVTDLRFTEANLAQLDALLAGVDLLYIEC
                     VFADEDRELARRRNHLTARQAGSIARRLGARQLQPFHFSPRYEGAEDRLRAQAVGAWQ
                     GVTEPDEPY"
     gene            complement(3329..3718)
                     /locus_tag="VIN75_22190"
     CDS             complement(3329..3718)
                     /locus_tag="VIN75_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976942.1"
                     /translation="MTELLLMLLVLFALAARTFQVRHRTAGWECLWRFRSGPCTVELR
                     RHAGLARLGGDSCEYPQPREFRVMALRIGGIPLWSQQAIISLPTDADSRIGEVPASEF
                     DHLFDRHFRMSWPRQAKAMRRLAARAH"
     gene            complement(3910..4809)
                     /locus_tag="VIN75_22195"
     CDS             complement(3910..4809)
                     /locus_tag="VIN75_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY8976943.1"
                     /translation="MTRHTGRIDDFGMDSISLAGPLPAKLRAIREAGFSQVMLSARDL
                     VGHAEGVQAAIAAVRDSGLRVTGFQVLRDFEGLSGQLHEYKVDIAKSMLELCAAVGAK
                     VLLVCSSTSAHASADRDAIARDLRKLAMLAIPRGIRVAYEALSWGRSIEEFPDAWDVV
                     MRADMPNLGLGLDSFHMFATKTSLDELQWLDPERIFLVQLADFMWQEIRSVEERIATA
                     RHFRVFPGEGAHSDQVAELVSRLHAQGYRGDYSFEVFNDDYQQIPLPDVARRARKAAD
                     WLGEGILRRSVPLPGAMRLQVVG"
     gene            complement(4806..>5581)
                     /locus_tag="VIN75_22200"
     CDS             complement(4806..>5581)
                     /locus_tag="VIN75_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8976944.1"
                     /translation="MGFRPIARHRSREVTLFRQGAMNVVVNASEKAPVDDEGVLDEAP
                     RIAAIALRVRDAAAAYRHVLDLGAWAVPMHVEVMELNIPAIHGVGGSRIYFVDRHKEF
                     SIYDVDFTAIPTVDPHPPAIAGLHWFGIVQYIGNDRMQDWTAFYSMLFGFAVLPDDQR
                     FGVMPKGRVLVGPGGPASRFYVQLIEPEPGIVDAEGREWIARIGLGTPDPRACAALLR
                     ERGVQFTETPHVQADDRGAVTRTQLGSVVFELVRDGEPS"
BASE COUNT          796 a         1900 c         2075 g          810 t
ORIGIN      
        1 agcgactgca ccatgcggcg gaactcgacc acgcgcgtcg agccgtcggc caccgacgtg
       61 gcgtagctgc cttccggcgc ggtgaagtgg aacgggtcgt agccccagtt gtagcagtcg
      121 gtcgaggccg tggcagcgac ggtggcctgc tgcgaggtcg agtccggcgc gccgcccggg
      181 ctcggcgtcg tgcagccgat ctccgggatg ctcgagatgt cgaagctggg catcaggtgc
      241 acgtcggtca gccccgcctg cgcgagcgcg cgcaggtggc gcatgccgtt cgagttcacg
      301 tcggtgaagg ccatgaactt gccgcggtgc gcggcgggca cggtggggtc gttgtcggag
      361 aagtcgcgca cgtgcagctc gtagatcgac atgtcggtcg atccggcgac ggtctgcggc
      421 gggcgggaga agtcccagcc cggcggcttc aggcgcggcg acgacaggtc ggtgacctgg
      481 ctgcgcaggc tgttggccga caggctcagc gagtacgggt cggtgacgat gttgcgcacc
      541 acgcccgtgc cgcgcacgaa cacctgcacg ccgtacatgt aatacaggcc gtcccactcg
      601 ccggggacgg tcaggctcca gatgccggtg ctggcgtcga aggccatcgg cagcgcgagc
      661 ctggcgccgg ccgtcgcgcc gaggtaggcg tacagcgaca cgttgcgcgc ggtcggcgcc
      721 cacaggcgga acgtggtgcg cccgtggtcg accgtcgcgc ccagcggagc ggtcgaggcg
      781 gcgggcgcgt acaggtcgtc cagcgcgccg gccagttgcg cggtggtggc gttctggatc
      841 gcgccggtcg catcctcctg caccagcacc acctggttgg tgagcagctt cttcaacagc
      901 ggcgcatccg tggcgcgcac ctggaacacg ccgccgttgc cgacatactt gaagcgcgtc
      961 gcggccgccg cgggcacggc gccggtgtag gggtcgagcg tgatcgagcc gtcggcgccg
     1021 gcgacgggct ggtccttggc ggcgacgatc gcggccgtgg ccgaccagta cagcttgacc
     1081 ggaccgtccg cggccacgcg gggccacttc acgagcgagc cgttcagcca gacggcgcgc
     1141 gcgtcggtcg tcgagaccga gcgcaggtcg ggctgggccg tgtagaccgt cgcgtcctgc
     1201 tcgacgagcc agacctggcc gacctggtcc gcgatggcca ggctgccgaa gtcgacatgg
     1261 atgttgttgg gacggccgtc cttgtcgttg atgttgggcg gcatgccgtg gatcaggaac
     1321 tcgatcgacg tcgtgcccgg attgctgcgc gggttcagca ccgggatgtc gaacacgatc
     1381 tcgccgccgc cgggcgcgta gccgggcatc gcgctgaatg gcgtcgggtt gttccagtcg
     1441 ccatagctga tcgtgcccat gcgcgaggtg tccaggccgc tgccgcccca caggtgcagg
     1501 ccccagttcg cgtagtcgct ggcgaagcgg atgtagtgga cgcgcagcgt cgagatgtcg
     1561 ggcgcgggcg aggtcagcgg gttgctcgcg taggtcgtga ggtcgccctc gacgcgccag
     1621 atctcgttgg cgccgttcac cagcacgtag gactggtcgg cgccgttgtc gtccttggtg
     1681 tcgccgtcgt ggaacaggta gccgacggtg ccggacgagg cggccagggg cacgtcgtag
     1741 tagacgccga agtcgtcggt gccgctggcg tcccagccgc cgttccaggc cgggctctgg
     1801 gccgcgttcc aggtgtggat ctgccagccg ctgtagtcgg agttcaggcg gtggtagtgg
     1861 acgcgcaggc tggtggcgac gacgtcggcc accttcctgc ggccggagcc ggccgcggtg
     1921 gcggcgatcg tcgacggcgg caccgcgtgc agcaccgtct gcgccgtggc cgcgttggcg
     1981 gcctccagcg tgtcgtcgcg ggcggggctc gcggtgtcgg cgccgccgcc gcaggccgcc
     2041 aggatgctcg cggcgagcat cacgagcgcg atgccgcgca agccgcggaa cgccgtctcc
     2101 agggtcttgt tcatggttgc ctcacgccac gcggcgccgc gccacgatgg gcggcgacct
     2161 cgttgtcgcg gcatcctaat gaagtaaaac tacacaacca agctcgggct taccctggct
     2221 ttgtgttgta actacattcg cttgtagtca atagggttcg tccggctcgg tgacaccctg
     2281 ccaggcgccg acggcctggg cgcgcaggcg gtcctcggcg ccttcgtagc gcggcgagaa
     2341 gtggaacggt tgcagctgcc gtgcgccgag gcgccgcgcg atgctgccgg cctggcgcgc
     2401 ggtcaggtgg ttgcgccgac gggcgagctc gcgatcctcg tcggcgaaca cgcactcgat
     2461 gtacagcagg tcgacgcccg ccagcagcgc atcgagctgg gcgaggttgg cctcggtgaa
     2521 gcgcaggtcg gtgacatagc cgatgcgccg gccgggcgtc gtgtccagca gcagcgggcg
     2581 cagttcggcg agccggaagc ggcgctcatg gtcgcccgcg gcgtcgcgcc agcgcacgtc
     2641 gagcgtgtcg tcgtccggcg cgccggccag cacggcggcc ttgagcgcct gcagccaggc
     2701 gccggtggcc aggcccatcg cggcgatgcg gtccgccgcc acgcgcacct gggccgtctc
     2761 ctcgacggca caggccagca cgggcatctc gtggtcgacg atgcgggcgc gcaggcgcag
     2821 cagcggctcc tcgagcagga cgtcgccgcg cgccggttcg gcgggcaggt cgcggcgcgc
     2881 gaacgcctcg cggctgtcga agcgggcgcc gcaccggctg ccgtcggcct ccagcgagcg
     2941 cgcctcgatg acgagcggga cggcgtagcg atggaccacg ttccacgtgt aggcgcgcag
     3001 cttgtggtcc accttgtcga cgaagcccgg cccgccccac accgtcatgc cgggcatgcg
     3061 gcccagcgcg gtggccagcc agtggtcgaa gccggcgaag tggtccatgt gcgggtgcgt
     3121 gacgaccgcg tgcgtgctgc gcagcagcgt gcgcggcgac agtgcgccca gctcgccgag
     3181 gtcgaacagc agcgcccggc gctggtcgcg cacggcgatc cacagcccgg gctcgcggcc
     3241 cgagggatcg acgagatggg ggtcgagcac cgtgcgcatg gcgtgctcct cacggcaagc
     3301 ccggcgcccg gcgggcgcgc gtgacgcgtc aatgcgcgcg agcggccagc cggcgcatcg
     3361 ccttggcctg gcgcggccag gacatgcgga aatggcggtc gaacaggtgg tcgaactcgc
     3421 tggccgggac ctcgccgatg cgcgagtcgg cgtcggtggg caggctgatg atggcctgct
     3481 gcgaccacag cgggatgccg ccgatgcgca gcgccatcac gcggaactcg cgcggctgcg
     3541 ggtattcgca ggaatcgccg ccgagccggg ccaggccggc gtggcggcgc agctccaccg
     3601 tgcagggacc ggagcggaag cgccagaggc attcccagcc ggcggtccgg tgccggacct
     3661 ggaacgtgcg cgcggccagt gcgaagagca ccagcaacat cagcagcagt tcggtcatgg
     3721 gcggcctcct tccgggcgga attccacggg atcaatgtgc tcctcttcgg gcaggaaggc
     3781 atcccaccaa agggtgcttc gatgcgccgg ggccacgttt cccgtgacgg accgcacata
     3841 ccgacggccg tattcacctg gttacagggc ctgcgtcgct ccccgcgccg gcctcggccc
     3901 gcgccggact cagcccacca cctgcagccg catcgcgccg ggcagcggca ccgatcggcg
     3961 caggatgccc tcgcccagcc agtcggcggc cttgcgcgcg cggcgcgcga cgtcgggcag
     4021 cgggatctgc tggtagtcgt cgttgaagac ctcgaagctg tagtcgccgc ggtagccctg
     4081 cgcgtgcagg cgcgagacga gctcggccac ctggtcggaa tgcgcgccct cgccgggaaa
     4141 cacgcggaag tggcgggcgg tggcgatgcg ctcctcgacc gagcggatct cctgccacat
     4201 gaagtcggcc agctgcacga ggaagatgcg ctcggggtcg agccactgca gctcgtccag
     4261 cgaggtcttg gtcgcgaaca tgtggaacga atccaggccc aggccgaggt tgggcatgtc
     4321 ggcacgcatc accacgtccc acgcatcggg gaactcctcg atgctgcggc cccacgacag
     4381 cgcctcgtag gcgacacgga tgccgcgcgg gatcgccagc atcgccagct tgcgcaggtc
     4441 gcgcgcgatc gcgtcgcggt cggcgctggc gtgcgcggag gtcgacgagc acaccagcag
     4501 caccttggcg ccgacggccg cgcacagctc cagcatcgac ttggcgatgt cgaccttata
     4561 ctcgtgcaac tggccggaca gcccttcgaa gtcgcgcagc acctggaagc cggtgacgcg
     4621 caggccgctg tcgcgcaccg cggcgatcgc ggcctggacg ccctcggcgt ggccgacgag
     4681 gtcgcgcgcg gacagcatca cctgcgagaa cccggcctcg cggatcgccc gcaacttggc
     4741 cggcagcggg ccggccagcg agatgctgtc catgccgaag tcgtcgatgc ggccggtgtg
     4801 tcttgtcatg aaggttctcc gtcacgcacg agttcgaaca cgacgctgcc cagctgcgtg
     4861 cgcgtcacgg cgccgcggtc gtccgcctgc acgtgcggcg tctcggtgaa ctgcacgccg
     4921 cgctcgcgca gcagcgctgc gcaggcgcgc gggtcgggcg tgcccaggcc gatgcgcgcg
     4981 atccattcgc ggccctcggc gtcgacgatg cccggctcgg gctcgatcag ctgcacgtag
     5041 aagcggctcg ccgggccgcc cgggcccacc agcacgcggc ccttgggcat cacgccgaag
     5101 cgctggtcgt ccggcagcac ggcaaagccg aacagcatcg aatagaacgc cgtccagtcc
     5161 tgcatgcggt cgttgccgat gtattgcacg atgccgaacc agtgcagccc cgcgatcgcc
     5221 ggcgggtggg gatcgaccgt ggggatcgcc gtgaagtcga cgtcgtagat cgagaactcc
     5281 ttgtgccgat cgacgaagta gatgcgcgag ccgcccacgc cgtggatggc cgggatgttg
     5341 agctccatca cctcgacgtg catgggcacc gcccaggcgc cgaggtccag cacgtggcgg
     5401 taggctgcag cggcgtcgcg cacgcgcagc gcgatcgcgg cgatgcgcgg cgcctcgtcg
     5461 agcacaccct cgtcgtcgac cggcgccttc tcgctggcgt tgacgaccac gttcatcgcg
     5521 ccctggcgaa acagcgtgac ctcgcgcgag cggtggcgcg cgatcggccg gaagcccatc
     5581 g
//
LOCUS       DATCRO010000814         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_538385, whole genome shotgun sequence.
ACCESSION   DATCRO010000814 DATCRO010000000
VERSION     DATCRO010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538385"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VIN75_22205"
     CDS             complement(<1..1143)
                     /locus_tag="VIN75_22205"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY8976945.1"
                     /translation="MNKTPLALMLACLLSGRAIAAPSTHEQLTTLAQDIVRIGAQRNP
                     VQATYLGIAGMDDQLVNPTEATRAAAIAQLNAWGRQLDAIRKTAGSSISLVDADDAKL
                     LRAQLDEYLDGLLVRQTDRKDYAGPALTLVDVIYTQFLHLPIPGRDDATAADVPRAWA
                     DIIARLEKGSAFIEAGQKLVTHPGKLYGVAGSKQIDGAPDFLNGALTDAAREQLANDP
                     ASLKRFVAARDAVLATLTKTNATIKAHVDTWPENFAMGRAAYDRMLQREQLLPYTGKD
                     IEGMARDELAHGWAEEAWVKAEAAHRKVALGAESGGGMAPSGPALIDYYRDRIAELRK
                     FVEEHDVVTLPAWLGSLDVVETPKFQQPVSPGASMESPRLFSKSGNG"
     gene            complement(1200..1760)
                     /locus_tag="VIN75_22210"
     CDS             complement(1200..1760)
                     /locus_tag="VIN75_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8976946.1"
                     /translation="MTASTTAAPPRERILRTAHDLFYRDGIRATGIDRVIAESGVAKV
                     TFYRQFPSKDDLILAFLDFRHERWMAWFVDALARHGGGVSAIAPALAEWFRGGDYRGC
                     AFLNSVGELAPALPAVLAHARRHKQDMTAAVARLMRPAPGRARVARALALAIDGAIVQ
                     AQYLASPEPAVEALACVTKALSKDLA"
     gene            1852..2319
                     /locus_tag="VIN75_22215"
     CDS             1852..2319
                     /locus_tag="VIN75_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8976947.1"
                     /translation="MDPKPPLPPFTRESAARKVRMAEDAWNSRDPARVAMVYTEDTRW
                     RNRAEFPVGRQQVREFLERKWARELDYRLIKELWAHDGNRIAVRFAYEWHDDSGHWFR
                     SYGNENWEFDDQGYMMRRFASINDLPIAEVDRKYHWPLGRRPDDHPGLSELGL"
     gene            2407..2826
                     /locus_tag="VIN75_22220"
     CDS             2407..2826
                     /locus_tag="VIN75_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY8976948.1"
                     /translation="MAPFRLAVLRAAVLAACALCAAGAIAESLQLTGAQEVPPNDSKA
                     TGTGDITIDADGNVSGSVSTPTIQGKMAHIHVGAPGKAGPPIITLSGGDQGTWSVPQG
                     ARLNAEQMAAWKAGNLYVNVHTAEHPGGELRAQLKAP"
     gene            complement(2827..3627)
                     /locus_tag="VIN75_22225"
     CDS             complement(2827..3627)
                     /locus_tag="VIN75_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8976949.1"
                     /translation="MSRIRVGIGGWTYEPWRDNFYPARLPAARELQHASRQVTAIEVN
                     GTYYSTMTAKAFERWFAETPDEFMFSLKANRFATNRRVLAEAGESIERFIGSGLSRLE
                     HKLGPILWQFAPTKKFDPADFEAFLKLLPADVQGRALRHVLDVRHDSFMAPEFLALAR
                     QYRCAVVFTDSPDYPSFANLTTDFVYLRLMNAKSDEPTGYPPAVLASFAACARAWAGG
                     GEPSGLPLVQGDAGAPPAASRDVFMFMINGAKERAPAAAMGVLAELRA"
     gene            complement(3677..4180)
                     /locus_tag="VIN75_22230"
     CDS             complement(3677..4180)
                     /locus_tag="VIN75_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976950.1"
                     /translation="MRFLVPSLLLACTMHTASAATAAQPADLFDFWLGDWNVSWHNAD
                     GTTGKARNHVSRILDGNVIEERFEQDAADPAPLLKGRSLSVRDPAGLWHQAWADNQGG
                     FFALTGSADGDKRLFSTALAVVGGEVTGKRMVFHHITADAFTWDWEGTADGGKTWKLL
                     WRLDYRR"
     gene            4393..4647
                     /locus_tag="VIN75_22235"
     CDS             4393..4647
                     /locus_tag="VIN75_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976951.1"
                     /translation="MNRRYAYRDYAILVSAQPATGAAGWRPEICVIAPDDRWEFVPTH
                     HSLVANDPQYCLEIGRRCAESAIQSMDPVREKAAYPGLLH"
     gene            complement(4712..5410)
                     /locus_tag="VIN75_22240"
     CDS             complement(4712..5410)
                     /locus_tag="VIN75_22240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976952.1"
                     /translation="MICASPDLSARDRTLAEHYRALLAQPGTDAADLQREEARWLRDV
                     RDTCPDEACIAQAYDVWNTLLLARSRRTASPAAEGETRPFPVDPALMDDARGLRGKAC
                     APGEDVPRDLGYLPVPGSLPVVGDGSVVLARQRIGAEFAVLLDTRRGCRMVDVVALPP
                     RLQAGPLLQCVVPSTDGSAAPLSYGIGLRPRGQKALLAYWEVDIDNGVLRRQPLGVLG
                     WGETIRCRQPEFGD"
     gene            complement(5407..>5699)
                     /locus_tag="VIN75_22245"
     CDS             complement(5407..>5699)
                     /locus_tag="VIN75_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976953.1"
                     /translation="LLQRFHGLLLQFQAAANLLPDRPADSKKVIISAIDGVAEAITEG
                     RDTVQGLRAPPQGAGDLADALRALAGDLASECGRRPAAPASTAPTRQAASRR"
BASE COUNT          812 a         2057 c         2029 g          801 t
ORIGIN      
        1 gccgttgccc gacttcgaga acaggcgcgg cgactccatc gacgcgcccg gcgacaccgg
       61 ctgctggaac ttgggcgtct cgacgacgtc cagcgagccc agccaggccg gcagcgtgac
      121 gacgtcgtgc tcctcgacga acttgcgcag ctcggcgatg cggtcgcggt agtagtcgat
      181 gagcgccggc ccgctgggcg ccatgccgcc gccgctctcg gcgccgagcg ccaccttgcg
      241 atgcgccgcc tcggccttga cccaggcctc ctcggcccag ccgtgcgcca gctcgtcgcg
      301 cgccatgccc tcgatgtcct tgcccgtgta cggcagcagc tgctcgcgct gcagcatccg
      361 gtcgtaggcc gcgcggccca tcgcgaagtt ctcgggccag gtgtcgacgt gcgccttgat
      421 ggtggcattc gtcttcgtca aggtcgcgag caccgcgtcg cgcgccgcca cgaagcgctt
      481 gagcgacgcg gggtcgttcg cgagctgctc cctggccgcg tcggtgagcg cgccgttgag
      541 gaaatcgggc gcgccgtcga tctgcttgct gcccgccacg ccgtacagct tgcccgggtg
      601 cgtcaccagc ttctggccgg cctcgatgaa ggcggagccc ttctccaggc gggcgatgat
      661 gtcggcccag gccctgggca cgtcggccgc ggtggcgtcg tcgcggccgg ggatcggcaa
      721 gtgcaggaac tgcgtgtaga tcacgtcgac gagcgtcagc gcggggccgg cgtagtcctt
      781 gcggtcggtc tggcgcacca gcaggccgtc caggtactcg tcgagctggg cgcgcagcag
      841 cttcgcgtcg tcggcgtcca ccagcgagat cgacgagccc gccgtcttgc ggatcgcatc
      901 gagctggcgg ccccacgcgt tcagctgcgc gatggcggcc gcgcgcgtcg cctcggtcgg
      961 gttcacgagc tggtcgtcca tgccggcgat gcccagataa gtggcctgca ccggattgcg
     1021 ctgcgcgccg atgcgcacga tgtcctgggc gagcgtcgtg agctgctcgt gcgtcgacgg
     1081 cgcggcgatc gcgcggccgg acagcaggca ggcgagcatc agcgccagcg gagtcttgtt
     1141 catgggaggg ttccgtggat cggatcgaag cgccggatgt tacgtccgcc gccaggcgat
     1201 caggccagat ctttcgacag cgccttcgtc acgcaggcga gcgcctccac ggccggctcg
     1261 ggcgaggcga ggtactgcgc ctgcacgatg gcgccgtcga tcgcgagcgc gagcgcccgc
     1321 gcgacgcgcg cgcgcccggg cgcgggacgc atcaggcgcg cgacggcggc ggtcatgtcc
     1381 tgcttgtggc gacgcgcgtg agccagcacc gccggcagcg cgggcgccag ctcgcccacg
     1441 ctgttgagga aggcgcagcc gcggtagtcg ccgccgcgga accattcggc caacgcgggc
     1501 gcgatggcgg acacgccgcc gccgtgacgc gcgagcgcgt cgacgaacca ggccatccag
     1561 cgctcgtggc ggaagtccag gaacgccagg atcaggtcgt ccttgctcgg gaactgccgg
     1621 tagaaggtga ccttggccac gcccgactcc gcgatcacgc gatcgatgcc ggtggcgcgg
     1681 atgccgtcgc ggtagaacag gtcgtgcgcg gtgcgcagga tgcgttcgcg cggcggcgcg
     1741 gcggtggtcg atgcggtcat gggcccattg tagacagacc tgtctacacg gcatagactg
     1801 cgccgcaggt agacgatcct gtctacatcg accctttcca ccggagaccc catggatccc
     1861 aagcccccgc tgccgccctt cacacgcgag tccgccgccc ggaaggtgcg catggccgag
     1921 gacgcttgga actcgcgcga cccggcgcgg gtcgcgatgg tctacaccga ggacacgcgc
     1981 tggcgcaacc gcgcggagtt ccccgtcggc cgccagcagg tgcgcgagtt cctggagcgc
     2041 aagtgggcgc gcgagctcga ctaccggctg atcaaggagc tgtgggcgca tgacggcaac
     2101 cgcatcgcgg tgcgcttcgc ctacgagtgg cacgacgatt cgggccactg gttccgctcc
     2161 tacggcaacg agaactggga gttcgacgac cagggctaca tgatgcggcg cttcgccagc
     2221 atcaacgatc ttcccatcgc cgaggtcgac cgcaagtacc actggccgct cggccgccgg
     2281 cccgacgacc atcccggcct gagcgagctg ggcctgtagc gcgcctcggt cgatttgacc
     2341 ctgcgctggt gtcgccgcgc atcgcgggtc accatggcgt cccctgacct gccctgagga
     2401 agcgccatgg ccccattccg tctcgcggtg ctccgcgccg ccgtgctcgc cgcctgcgcc
     2461 ctgtgcgcgg ccggcgccat cgccgaaagc ctgcaactga ccggcgccca ggaggtcccg
     2521 cccaacgaca gcaaggccac gggcaccggc gacatcacga tcgacgccga cggcaacgtg
     2581 agcggcagcg tgtccacgcc cacgatccag ggcaagatgg cgcacatcca tgtcggcgcg
     2641 cccggcaagg ccggcccgcc gatcatcacg ctcagcggcg gcgaccaggg cacctggagc
     2701 gtgccgcagg gcgccaggct caatgccgag cagatggccg catggaaggc cggcaacctc
     2761 tatgtcaacg tccacaccgc cgagcatccc ggcggcgagc tgcgcgcgca gctcaaggcg
     2821 ccctgatcag gcccgcagtt cggccagcac gcccatggcc gcggccgggg cgcgctcctt
     2881 ggcaccgttg atcatgaaca tgaagacgtc gcgcgacgcc gcgggcggcg cgcccgcgtc
     2941 gccctgcacc agcggcaacc cgctcggctc gccgccaccg gcccacgcgc gcgcgcaggc
     3001 cgcgaacgac gccaggaccg cgggcgggta gccggtcggt tcgtcggact tcgcgttcat
     3061 caggcgcagg tagacgaagt cggtggtcag gttcgcgaac gacgggtagt cgggcgagtc
     3121 ggtgaacacc accgcgcagc ggtactggcg cgccagcgcg aggaactccg gcgccatgaa
     3181 gctgtcgtgg cgcacgtcca gcacgtggcg cagcgcgcgg ccctgcacgt cagccggcag
     3241 cagcttcagg aaggcctcga agtcggccgg gtcgaacttc ttggtcggcg cgaactgcca
     3301 caggatcggg cccagcttgt gctcgagccg cgagaggccg ctgccgatga agcgctcgat
     3361 cgactccccc gcctcggcca gcacgcggcg gttcgtcgcg aagcggttgg ccttgagcga
     3421 gaacatgaac tcgtcgggcg tctcggcgaa ccagcgctcg aaggccttgg ccgtcatcgt
     3481 gctgtagtag gtgccgttga cctcgatggc cgtgacctgc cggctcgcgt gctgcaactc
     3541 gcgcgcggcg ggcagcctgg cggggtagaa gttgtcgcgc cacggctcgt acgtccagcc
     3601 tccgatgccg accctgatcc tgctcatcgc tgcgctcctc gttggggaac gcgtttttac
     3661 cccatcgtgc gccgactcac cgccggtaat cgagtcgcca gagcagcttc caggtcttgc
     3721 cgccgtcggc ggtgccttcc cagtcccagg tgaatgcatc ggcggtgatg tggtggaaga
     3781 ccatgcgctt gccggtgacc tcgccgccga ccacggccag ggccgtcgag aacagtcgct
     3841 tgtcgccatc ggcgctgccg gtgagcgcga agaagccgcc ctggttgtcg gcccaggcct
     3901 ggtgccacaa cccggccggg tcgcgcacgc tcagcgagcg gcccttgagc agcggcgccg
     3961 ggtcggcggc gtcctgttcg aagcgttcct cgatgacgtt gccgtcgagg atgcgcgaca
     4021 cgtggttgcg cgccttgccg gtcgtgccgt cggcgttgtg ccaactgacg ttccagtcgc
     4081 cgagccagaa gtcgaacagg tcggccggtt gcgcggcggt ggcggcggaa gcggtgtgca
     4141 tcgtgcaggc cagcaggagc gaagggacga ggaatcgcat gggaaacctc ccggtggggt
     4201 gcgggcctcc gttgtagcgc cggggccggc gccccgcccg gccgttgcga ccggcgacgg
     4261 ccggcggggg gcgagcggcg gcccggccgc gccgaaatcc gggattggcc ggaaatacgc
     4321 acgtttctcg cccgatcgga atccatgccg gccgccacac tgggcgttcc cgccccagac
     4381 cgcccaccgg acatgaaccg tcgatacgcc taccgcgact acgccatcct ggtctccgcc
     4441 caaccggcca ccggcgccgc gggctggcgt cccgagatct gcgtgatcgc ccccgacgac
     4501 cgctgggaat tcgtgccgac gcaccactcg ctggtggcca acgacccgca gtactgcctc
     4561 gagatcggcc gccgctgcgc cgagagcgcc atccagagca tggatcccgt gcgcgagaag
     4621 gccgcgtacc ccggcctgct gcactgaccg gccgccgcgc ggccacccga ccaggcgccc
     4681 gcgaggcgcc tttttcacgc ccgcgcgcgg ctcagtcgcc gaactccggc tggcggcagc
     4741 ggatcgtctc gccccagccc agcacgccca gcggctggcg ccgcagcacg ccgttgtcga
     4801 tgtcgacctc ccagtacgcg agcagcgcct tctgcccgcg cgggcgcagg ccgatgccgt
     4861 aggacaaggg cgcggccgag ccgtcggtcg acggcacgac gcactgcagc aggggccccg
     4921 cctgcaggcg cggcggcagc gccacgacat ccaccatccg gcaaccccgc cgggtatcca
     4981 gcagcacggc gaactcggcg ccgatgcgct ggcgcgcgag caccacgctc ccgtcgccca
     5041 ccaccggcag cgatccgggc acgggcaggt agccgaggtc gcgcggcacg tcctcgcccg
     5101 gcgcgcaggc cttgccgcgc aggccgcgcg cgtcgtccat cagcgccgga tcgaccggga
     5161 acggccgggt ctcgccctcg gccgccgggc tggcggtgcg gcggctgcgc gcgagcagca
     5221 gcgtgttcca cacgtcgtaa gcctgcgcga tgcaggcctc gtcggggcag gtgtcgcgca
     5281 cgtcgcgcag ccagcgcgcc tcctcgcgct gcaggtcggc cgcatccgtg ccgggctggg
     5341 ccagcagcgc gcggtaatgc tcggccagcg tgcggtcgcg cgccgacagg tcgggcgacg
     5401 cgcagatcat cttcgtgacg cggcctgtcg cgtgggcgca gtcgaagctg gggccgccgg
     5461 ccgcctgccg cattcgctgg ccaggtcgcc agcgagcgcc cgcaaggcgt ccgcgagatc
     5521 gcccgcgccc tgcggcgggg cgcgcaggcc ttgcacggtg tcccggccct cggtgatcgc
     5581 ttcggccacc ccgtcgatgg cgctgatgat gaccttcttg gaatcggcgg ggcggtcggg
     5641 aaggaggttg gccgcggcct ggaactgcag cagcagcccg tgaaagcgct gcagcaagg
//
LOCUS       DATCRO010000815         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1066468, whole genome shotgun sequence.
ACCESSION   DATCRO010000815 DATCRO010000000
VERSION     DATCRO010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066468"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..2062)
                     /locus_tag="VIN75_22250"
     CDS             complement(164..2062)
                     /locus_tag="VIN75_22250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8976954.1"
                     /translation="MNPLHATARRRPGRRLVALALSWLLLAAACLAAGAAHAVEPVNV
                     RVHFHRDKADYAGWGLHVWGAGLVLPHSVSWDRPLEAAGVDKYGVYFDVGVEPTVQTF
                     NLIVHHGETKSTPRDLAVDIASQGHEVWVREGSDSVFTSPPPVDAPYGPGVDAERQQR
                     ESLQWIAGGAVGAVALLVLGWRFVSRRMSSTREQLAANMQLLLQARNDLRAQGERLQG
                     LVTDELTCLPTRGGLQQALEQALARASRGDQAVGVMFIDLDGFKQVNDSGGHDAGDEV
                     LRTVARRLRATVRECDFVARVGGDEFVVLLEAVASPMRAFLVGRKLVRAIGEDIPFAG
                     QAWRVGASVGIALSPGDGADAAALLKSADTAMYETKRAGKGACRFVQPARQAEIERQL
                     ALEQALRRSLAGDELRLALEPVVELATGRVAGHQAAIGWQVGAHAVDVQAVVDGADDA
                     ALAQQVDHWLLTRACRAAVLERAADGAPGFVSVSLGAAAAELQDLPALVRDVLAEEGL
                     PPQRLLLKFPGHRLADPQRSLDVLLRLRGQGVRIGFSGITELDIGLQRLVLAPVDQLE
                     LDVRSGSASQRGTPYVRALAAVGAQCGYSMAATGIATAAQRRWVEAAGCTFGVGPACS
                     ERVETQTA"
     gene            complement(2225..3091)
                     /locus_tag="VIN75_22255"
     CDS             complement(2225..3091)
                     /locus_tag="VIN75_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8976955.1"
                     /translation="MAKHPSTFRIGGDIEIQRLGFGAMRITGKGIWGPPPDRAGALAT
                     LRRVPELGIDFIDTANSYGPHVSEELIREALYPYTEVVIATKGGLERSGPDVWTQNGN
                     PSHLVSEAHASRERLGVDRIPLWQLHRIDAKVPRDEQFDAVRLLLDDGVIEHAGLSEV
                     SVEQIEAASRVFPVATVQNRYNFADRGSEDVLEYCEAHGIGFIPWYPLAAGELTREGT
                     AALDAVAKAHGATPSQVAVAWLLRRSPVIIPIPGTSKVAHLEENVKARDIELTAEEFD
                     RLDRVGRKPGHG"
     gene            complement(3392..>3821)
                     /locus_tag="VIN75_22260"
     CDS             complement(3392..>3821)
                     /locus_tag="VIN75_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin reductase"
                     /protein_id="HEY8976956.1"
                     /translation="IQLWTNTELVELQGQLPSGLSSVKWRQGGKDGQVHERELRNVFL
                     FVGADPETRWLEGCGIVVDKHGFVLTGVEAATGADGHVKPGRVPNPFETSIRGAFAVG
                     DVRAGSVKRVGGAIGDGAAAVALIHQHLAALDAKTPSEQA"
BASE COUNT          499 a         1413 c         1362 g          547 t
ORIGIN      
        1 cccgccgatg ggctaccggc tgaaggatcg cgacacgccg gtgttttttc cgcgcacgtt
       61 cagcccgcgg gggtgatgcg cagaaggggt ccgcggaccg cgcggttcga ggagttgcca
      121 ggccccttcc ggcaacgcgt cccgatacgc cacgcttccc ggctcacgcc gtctgcgtct
      181 ccacccgctc gctgcacgcc ggccccaccc cgaacgtgca gcccgcggcc tcgacccagc
      241 ggcgctgggc ggccgtcgcg atgcccgtcg ccgccatcga gtaaccgcat tgcgcgccca
      301 cggccgccag ggcgcgcacg tagggcgtgc cgcgctggct cgcgctgccg gagcgcacgt
      361 cgagctcgag ctggtcgacc ggcgcgagga cgagccgctg caggccgatg tccagctcgg
      421 tgatgccgga aaagccgatg cgcacgccct ggccgcgcag gcgcagcagg acatcgagcg
      481 agcgctgcgg gtcggccaag cggtggccgg ggaacttcag cagcaggcgc tgcggcggca
      541 ggccttcctc ggccagcacg tcgcgcacca gcgcgggcag gtcctgcagc tccgcggccg
      601 cggcgccgag cgacacggac acgaaccccg gcgcgccgtc ggccgcgcgc tcgagcacgg
      661 ccgcgcggca ggcgcgcgtg agcagccagt ggtcgacctg ctgcgcgagc gcggcgtcgt
      721 cggcgccgtc gaccacggcc tggacgtcga cggcatgcgc acccacctgc cagccgatgg
      781 ccgcctggtg gcccgcgacg cgccccgtcg cgagttcgac gaccggctcc agcgcgaggc
      841 gcagctcgtc gccggccagc gagcgccgca gcgcctgttc cagcgcgagc tggcgctcga
      901 tctccgcctg gcgcgccggc tgcacgaagc ggcacgcgcc cttgccggcg cgcttggtct
      961 cgtacatcgc ggtgtcggcc gacttcagca gcgcggccgc atcggcgccg tcgccgggcg
     1021 acagcgcgat gcccacgctc gcgcccacgc gccaggcctg gccggcgaag gggatgtcct
     1081 cgccgatcgc gcgcacgagc ttgcggccga cgaggaacgc gcgcatcggc gaggccacgg
     1141 cctccagcag cacgacgaac tcgtcgccgc cgacgcgcgc cacgaagtcg cactcgcgca
     1201 cggtcgcccg caggcggcgc gcgaccgtgc gcagcacctc gtcgcccgcg tcgtggccgc
     1261 cgctgtcgtt gacctgcttg aacccgtcga ggtcgatgaa catcacgccc accgcctggt
     1321 cgccgcgcga tgcgcgcgcc agcgcctgct cgagcgcctg ctgcagcccg ccgcgcgtgg
     1381 gcaggcaggt gagttcgtcg gtcaccaggc cctgcagccg ctcgccctgc gcgcgcaggt
     1441 cgttgcgtgc ctgcagcagc agctgcatgt tggcggccag ctgctcgcgc gtgctgctca
     1501 tgcggcggct gacgaagcgc cagcccagga cgagcagcgc cacggcgccg accgcgccgc
     1561 ccgcgatcca ctgcagcgac tcgcgttgct ggcgttcggc gtcgacgccc gggccgtagg
     1621 gcgcgtcgac cggcggcggc gacgtgaaca cgctgtcgga tccctcgcgc acccagacct
     1681 cgtggccctg gctggcgatg tcgaccgcca ggtcgcgcgg cgtgctcttg gtctcgccgt
     1741 ggtggacgat caggttgaag gtctgcaccg tcggctcgac gccgacatcg aagtacacgc
     1801 cgtacttgtc gacgcccgcg gcctccagcg gccggtccca gctgacgctg tgcggcagca
     1861 ccaggcccgc gccccacacg tgcaggcccc agccggcgta atcggccttg tctcggtgga
     1921 agtgcacccg cacgttgacc ggctcgaccg cgtgcgcggc gccggccgcg aggcaggcgg
     1981 cggccaggag gagccaggac agggcgagcg cgacgagccg tcggccgggg cgccgccgcg
     2041 cggtcgcatg cagggggttc atggaccggc ttatcggcgc tccggcgccg cgctgaactg
     2101 ccgcgaaccc gcggcgggcg tcgtcggcgt gcactcgtcc acgttcgtgc ggggcacgcg
     2161 catccgcgcc cggcgcggat cgagcccatg tcaggtgacg agcccgacag gcgcgcgccg
     2221 gcgctcagcc gtggccgggc ttgcggccga cgcgatcgag ccggtcgaac tcctcggctg
     2281 tcagctcgat gtcgcgcgcc ttcacgttct cctccaggtg cgcgaccttc gacgtgccgg
     2341 gaatgggaat gatgaccggg ctgcgccgca gcagccatgc gaccgccacc tggctgggcg
     2401 tcgcgccatg cgccttggcg acggcgtcga gcgccgccgt gccctcgcgc gtcagctcgc
     2461 ccgcggccag cgggtaccac gggatgaagc cgatgccgtg ggcctcgcag tactcgagca
     2521 cgtcctcgct gccgcggtcg gcgaagttgt agcggttctg cacggtggcc accgggaaca
     2581 ccctggaggc ggcctcgatc tgctcgaccg agacctcgct cagccccgca tgctcgatga
     2641 cgccgtcgtc cagcagcagg cgcaccgcgt cgaactgttc gtcgcgcggc accttggcat
     2701 cgatgcggtg cagctgccac agcgggatcc ggtcgacgcc cagtcgctcg cggctggcgt
     2761 gcgcctcgct gacgaggtgg ctcggattgc cgttctgcgt ccacacgtcc ggcccgctgc
     2821 gttccaggcc gcccttggtc gcgatgacga cctcggtgta cgggtagagg gcctcgcgga
     2881 tcagttcctc ggacacgtgc ggcccgtacg agttggcggt gtcgatgaag tcgatgccca
     2941 gttcgggcac gcggcgcagc gtcgcgagcg cgccggcgcg gtccggcggc gggccccaga
     3001 tgcccttgcc ggtgatgcgc atggcgccga agccgaggcg ctggatctcg atgtcgccgc
     3061 cgatgcggaa cgtggaaggg tgcttggcca tgacggtcgt cctgtgctga tcgagaggac
     3121 ggtgccaggt cctgcctttt gcgcgttcga gcgtttcggg tggggtcagg tcttgaatgt
     3181 tgcgcacgaa gcgatggctg gatggtgtca ggtcttgaaa gttgcgcgcc atgacgcgtg
     3241 aagtccggcg ctgtcttgtc gcgcaacttt caagacctga caccatctct ccgctgtctc
     3301 gacgcgcaac attcaagacc tgaccccgac gccttgccgc gcaaaaggca ggacctgaca
     3361 ccgcgtttca cgccagtatg gtcgcgcgtc atcaggcttg ctccgatggg gtcttcgcat
     3421 ccagcgccgc caggtgctgg tggatcagcg ccaccgcggc ggcgccgtcg ccgatcgcgc
     3481 cgcccacgcg cttgacggag ccggcgcgca cgtcgcccac cgcgaacgcg ccgcggatgc
     3541 tggtctcgaa cgggttgggc acgcgtcccg gcttgacgtg gccgtccgcg ccggtggccg
     3601 cctccacgcc ggtcagcacg aagccgtgct tgtcgacgac gatgccgcag ccttcgagcc
     3661 agcgtgtctc ggggtcggca ccgacgaaca ggaacacgtt gcgcagctcg cgctcgtgca
     3721 cctgcccgtc cttgccgccc tggcgccact tcacgctgga cagccccgag ggcagctggc
     3781 cctgcagctc caccagctcg gtgttggtcc acagctggat g
//
LOCUS       DATCRO010000816         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1044372, whole genome shotgun sequence.
ACCESSION   DATCRO010000816 DATCRO010000000
VERSION     DATCRO010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044372"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2566)
                     /locus_tag="VIN75_22265"
     CDS             complement(<1..2566)
                     /locus_tag="VIN75_22265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8976957.1"
                     /translation="MHQRTKVGQGVALALATLVAASAHAQDVSDIQRVEITGSAIKRI
                     NVEGSLPIQTISQDDIKKSGVTTTADLIQNLPAMQGFTAESQSVNGGGGGATTASIHD
                     LGAAYTLVLLNGHRLASYTTGSEVNLDQIPLSAVDHVDVLTDGASALYGADAIAGVVN
                     IITKRDSTEGDFDVSFDRPLKAGGQSASASISKGFGDLAKDRYNIFLAASFDHQSALQ
                     AYQRPFSRTGLLSFNDRHGAQSVPLTSVYTPVANVYADTTSAGATSPDGSLFYNPSLA
                     ANGKCGGTHSIDAGDGSCAYDYASTVEDLPASRKANLFATGRFNVSDKVSLFAEALVG
                     SNRTDALYAAPAQSFSLNPAQIAQYVTPQLAAGTTSANEVGYLRMVGTGGRKDGYETR
                     TQHIVLGADVTLGKWDTTFSFTHSQNHWYDIAEGGYSSANAINALMDSGAFDPFSGDA
                     QADVLAPAVLHQTLEQSRSTLDVASAHGSTTLAALGGGDLSLATGLDLTRQKYTDRPS
                     AIEMGPNALQPNYTDTLFGGSSGTLPFDSARNAWGVFAEMDAPVTKQFEVDASARFDS
                     YSAVHNKDGFDANGDPSGAETQGRKSSATTFKLSGNFRPTGQWLMRGSFGTGFRMPTI
                     GDVSNPLEFFGVTGNETCSANVPDDLAQACHKSNGQYVPFQYNEQSGGNPSSGAGGLK
                     PERSQQWTLGARFEPSPAFSMGVDLWNVRIHDQINTITENTAFKDMAAYSYLFSVIDD
                     PATGAPTLTFLSQPINTGSAFYQGLDFDGQSVVNTPVGRLTTRGHVTWMLRADYQQPG
                     NPGYVNDMNKVGQDGQVTFRYQVNASTSLKQGHFTHTVAFNFKPGYKDDYTDYCWDT"
BASE COUNT          452 a         1016 c         1147 g          585 t
ORIGIN      
        1 ccgtgtccca gcagtagtcg gtgtagtcgt ccttgtagcc gggcttgaag ttgaacgcca
       61 ccgtgtgcgt gaagtggccc tgcttcagcg acgtcgacgc gttgacctgg tagcggaacg
      121 tcacctgccc gtcctggccg accttgttca tgtcgttgac gtagccgggg ttgcccggct
      181 gctggtagtc ggcgcgcagc atccacgtca cgtggccgcg cgtcgtcagc cggcccacgg
      241 gcgtgttgac cacgctctgg ccgtcgaagt ccaggccctg gtagaacgcg ctgccggtgt
      301 tgatcggctg cgacaggaag gtcagcgtgg gcgcgcccgt ggccggatcg tcgatcacgg
      361 agaacaggta gctgtaggcc gccatgtcct tgaaggcggt gttctcggtg atggtgttga
      421 tctggtcgtg gatgcgcacg ttccacaggt cgacgcccat cgagaacgcc ggcgacggct
      481 cgaagcgcgc gcccagggtc cactgctgcg aacgctcggg cttgaggccg ccggcgccgc
      541 tggacgggtt gccgccggac tgctcgttgt actggaacgg cacgtactgc ccgttggact
      601 tgtggcaggc ctgcgccagg tcgtccggca cgttggccga gcaggtctcg ttgccggtga
      661 cgccgaagaa ttccagcggg ttgctcacgt cgccgatggt gggcatccgg aagcccgttc
      721 cgaacgagcc gcgcatgagc cactggccgg tcggacggaa gttgccgctc agcttgaagg
      781 tggtcgccga gctcttcctg ccctgcgtct cggccccgct ggggtcgccg ttcgcgtcga
      841 agccgtcctt gttgtgcacc gcgctgtacg agtcgaagcg cgcggacgcg tcgacctcga
      901 actgcttggt gacgggcgcg tccatctcgg cgaagacgcc ccaggcgttg cgcgccgagt
      961 cgaacggcag cgtgccggag ctgccgccga acagcgtgtc ggtgtagttc ggctgcagcg
     1021 cgttcgggcc catctcgatc gcgctcgggc gatcggtgta cttctggcgg gtcaggtcca
     1081 ggccggtggc caggctcagg tcgccgccgc ccagcgccgc gagcgtcgtc gatccgtgtg
     1141 ccgaggcgac gtccagcgtc gaacggctct gctccagcgt ctggtgcagc acggccgggg
     1201 cgagcacgtc ggcctgcgcg tcgccggaga acgggtcgaa cgcgccgctg tccatcagcg
     1261 cattgatcgc gttggccgac gagtagccgc cctcggcgat gtcgtaccag tggttctgcg
     1321 agtgcgtgaa cgagaacgtg gtgtcccact tgccgagcgt gacgtcggcg ccgagcacga
     1381 tgtgctgggt gcgggtctcg tagccgtcct tgcggccgcc ggtgcccacc atgcgaaggt
     1441 agcccacctc gttcgccgac gtggtgccgg ccgccagctg cggcgtcacg tactgcgcga
     1501 tctgcgcggg gttcagcgag aaggactgcg ccggcgcggc gtacagcgcg tcggtgcggt
     1561 tgctgcccac cagcgcttcc gcgaacagcg acaccttgtc gctcacgttg aagcggccgg
     1621 tcgcgaagag gttggccttg cgcgacgccg gcaggtcctc caccgtcgac gcgtagtcat
     1681 aggcgcagga gccatcaccc gcgtcgatcg agtgcgtgcc gccgcacttg ccgttggccg
     1741 ccagcgacgg gttgtagaac agcgagccat ccggcgaggt ggcgccggcg ctggtggtgt
     1801 cggcatagac attggccacc ggcgtgtaga cgctggtcag cgggacgctt tgcgcgccat
     1861 gcctgtcgtt gaagctcagc aggccggtgc gcgagaacgg gcgctggtag gcctgcagcg
     1921 ccgactggtg gtcgaagctg gcggccagga agatgttgta gcggtccttg gcgaggtcgc
     1981 cgaagccctt ggagatcgac gccgacgcgc tctggccgcc ggccttgagc ggacggtcga
     2041 acgacacgtc gaagtcgcct tcggtgctgt cgcgcttggt gatgatgttg acgacgccgg
     2101 cgatcgcgtc ggcgccgtac agcgcggagg cgccgtcggt gagcacgtcg acgtggtcga
     2161 ccgcgctgag cgggatctgg tcgaggttga cttcggagcc ggtggtgtac gaggccaggc
     2221 ggtggccgtt cagcagcacc agcgtgtagg ccgcacccag gtcgtggatc gaggccgtgg
     2281 tcgcgccgcc gccgccgccg ttgaccgact gcgactcggc ggtgaagccc tgcatcgccg
     2341 gcaggttctg gatgaggtcg gcggtggtgg tgacgcccga cttcttgatg tcgtcctggg
     2401 agatcgtctg gatcggcagc gagccctcga cgttgattcg cttgatggcc gagccggtga
     2461 tctcgacgcg ctggatgtcg ctgacgtcct gcgcgtgggc ggacgccgcc accagcgtgg
     2521 ccagcgccaa tgcgacgccc tggcccacct tcgttcgttg atgcatggat tcctccggaa
     2581 agtttcgatt cggtaccgcg cgccgctgaa ctcatgattc aggcgcgcgt ccatggggat
     2641 gcggattttt cctgtcggag aaggcccgtg ccggcgcgca aaccctgatg tggcgaggcc
     2701 cccacgccac aagatgacat ttcggtcagg tgatttcgcg cggccgtgaa gcgttcgatc
     2761 ggatcggcga gcccatgtcc ccgccatgga tgagccggta ccgcggcggg tcgcgcatcg
     2821 ccgacgccgc gccgggtcgt cgtccgccaa ttggcggttt cgcctcgccg ggtttgctga
     2881 caggatcgtc agcgaaagca ggccttgcac ggtatccagc aaaggttctt aactcgcgcc
     2941 gcattgcatg gcatggctga catggagcaa agataaagcg ttgacaccat cctgcatcgc
     3001 gccactttca ccgacggaaa catgccggca cgacgccgtt gacgcggcgc gcgtgcaatg
     3061 acatcgatcg atggagcttg gggcccggcc gcgtgccggg cttttttctg cgcgatcagc
     3121 cggactggcg gctcctttcc tgactgctgc ggcagggaag gctgccgccg ggcgcttcgt
     3181 cccggccgat cgctttggcc
//
LOCUS       DATCRO010000817        11169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_762592, whole genome shotgun sequence.
ACCESSION   DATCRO010000817 DATCRO010000000
VERSION     DATCRO010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762592"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..384
                     /locus_tag="VIN75_22270"
                     /pseudo
     CDS             108..384
                     /locus_tag="VIN75_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206856.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
     gene            411..923
                     /locus_tag="VIN75_22275"
     CDS             411..923
                     /locus_tag="VIN75_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEY8976958.1"
                     /translation="MLIRTSLALSTAVAAVPALSAEATLRIQLPRLDIAEYHKPYVVA
                     WIEKASDQSFAGNVALWYDVNKRNDGGARWLKDMRSWWRKSGHDAPAVDGITGATRTA
                     GEHAINLLDAKAVAALPPGRYEVVVEAARENGGREVLRVPFEWPLNKLQSAKVQGQGE
                     LAAVAIDAKP"
     gene            935..1744
                     /locus_tag="VIN75_22280"
     CDS             935..1744
                     /locus_tag="VIN75_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY8976959.1"
                     /translation="MRHPCPRLALLAGGALLCLSAQAHRPWLLPSATLVDGPAPVVSI
                     DAAISDDLFEFDSLPLQLETLRVVAPDGRIVEPDGRIEAKRRISFDVELAQEGTYRIA
                     GVTDTTVASWKAGGQARRWRGALAALASAVPADAQDLQVTRQLARAETFVSHATPGPP
                     PAAITGEGLELQPLTSPTDLSVGDASRFRVLLDGRPFANADVTLIRGGNRYRYKMGEL
                     ALKTDAAGEFGVAWPEAGRWWLGINHGDRAQGGAAPARRESYSATFEVLPK"
     gene            1747..2742
                     /locus_tag="VIN75_22285"
     CDS             1747..2742
                     /locus_tag="VIN75_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8976960.1"
                     /translation="MAPRVLVPPAIGRTPPALGGRVHEFTGRTMGTTWSVRLVAASTP
                     WRAPVGEAIQSALDEVVAQMSTWDASSDLCRFNAAPPGTWHALSDHFHEVLACARDIA
                     AHSAGAFDPTAGALVDAWGFGPAPRRGVPDAATLAQACRSAGWRRIGIDPARRALRQP
                     GGLALDLSAIAKGFGVDLVARRLQAQGIADHLVEVGGELRGTGVKPDGQPWWVQLEQP
                     DGDGTGERTLLALHGLSVATSGDYRRWFEHEGVRYSHTIDPRDGQPIRHGLASVTVIH
                     ADCMRADAWSTALNVLGPDAGLALADRLGLAARFLVRDGEGFVERRSAAFDALLQ"
     gene            2739..4139
                     /locus_tag="VIN75_22290"
     CDS             2739..4139
                     /locus_tag="VIN75_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY8976961.1"
                     /translation="MTAFGVDGARLLGAAAVVVSYAALCLAVVWNERRKAANAAVRTK
                     ALAGGTNAKPLLIVHASQTGAAERLAWQAAHALRAAAMPLRVAAIGTLTTGELASASA
                     VLFIASTCGEGDAPDAALPFVRRVMDIAGTPSLAGLRYGLLALGDREFAHFCAFGRRL
                     DDWLREHGARPLFERIDVDAMDACALGRWKHELGRLGSVGDALAWHEPARETWRLARR
                     THVTPGSLGNPVFHLELEPPAGAAPHWEPGDLLQVRVPHAPDAPRPYSIASIPADGRA
                     HLLVRQQRRGDGQPGLASGWLTADAPIGGDVVLDLLPHRNFRLGDNARRPLILVGNGT
                     GIAGLRAHLRARASRAGAGPATRPRNWLVFGERQAAHDFHFRADIQAWQADGTLDRVD
                     LAFSRDQAPRCYVQDRLRAQLALVRQWLDDGAAVYVCGSLRGMAGGVEEALVEIVGDE
                     RLQALKAEGRYRRDVY"
     gene            complement(4338..5018)
                     /locus_tag="VIN75_22295"
     CDS             complement(4338..5018)
                     /locus_tag="VIN75_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY8976962.1"
                     /translation="MLLHVPDVLLSDQVADLRRRLADAEWVDGRATVGEQGARVKRNR
                     QLDEFSPTGQELGRVVLKALLANPLYLSAALPLRHMPPLFNCYEGGEHYGLHVDGAIR
                     SVPGTTLQMRTDLSCTLFLSGPHEYDGGELEIVDTYGTHEVKLPAGDLVLYPSSSLHR
                     VHPVTRGARVASFFWVQSLVRDTARRRALFELDQNIQALRAQVGDNEAVLGLTGHYHN
                     LVRAWSEV"
     gene            complement(5050..7347)
                     /locus_tag="VIN75_22300"
     CDS             complement(5050..7347)
                     /locus_tag="VIN75_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HEY8976963.1"
                     /translation="MRPIQSRKHAAARQPRHHSTVLLAATALALPGLVLAQSAAVPAA
                     SASQPRGETTLPTVKVQAAAPENDYKADVVSSPKFTQPIVDTPQTITVIRKELLQQQA
                     ATSLSEALRNTPGVTMQLGENGSTQTGDSINLRGFDTSQSIFVDGIRDLGAVSRDVFD
                     IDQVEVVKGPSGSDIGRGASSAYINLVSKTAQADGGFATGSLVAGTADRKRLVADFNT
                     PLASVPGSAVRLNVMAQDDGTPGRDEVRQKRWGLAPTATFGLGSSTRETISLLHIQQD
                     NRPDGGVSTFGLDGYVFAAATATAPAQQPGPAVDPGKFYGLRSDFDHVKVDMFTSMLE
                     TDLAKGVVLRNTTRIGRTVQDVVVTGVNAVTATAADPSDYTVARSRQGRHQDNEILAH
                     QANLSTQFETFGLGSALSSGFELSWERQRTATPVTVAGSASLYAPNPDDAMAMPSYKG
                     TQTIGSTLTVSAYAFETLDVTDALLLNAGARVDRFHTDTSGATYTAASGATPAALANN
                     APLGLTDTLWSWKVGAVFKPAKTGSVYVTYSNSRNPPGGTNFALSSQASSAAQPGLKP
                     QEGTNIEFGTKWDLLGGDLLATAAVYRSENSNELVSDGATVPTYAQVGKRRVQGVELG
                     LVGKVAKDLSVSLGYSNMDSTIVRGAPAQQGGVIVFSPRNTFTSWVTARLPLGVTLGG
                     GMRYADTSARSSNTAAPTANLLNAPAYWVGDAMASYELSRHWQVQLNVYNLFDRKTMQ
                     SLNNGGSRYTPGASRNALVSLDAKF"
     gene            7346..7501
                     /locus_tag="VIN75_22305"
     CDS             7346..7501
                     /locus_tag="VIN75_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976964.1"
                     /translation="MDLSFERWRGTFRLASTALVSDEILLEMLARPGEPDRSQMLMIL
                     INKVNVE"
     gene            7616..7879
                     /locus_tag="VIN75_22310"
     CDS             7616..7879
                     /locus_tag="VIN75_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY8976965.1"
                     /translation="MATAATEKSSAFMKALKPSPALAAIVGSEPLPRTEVTKRLWDYI
                     KAHNLQNPANKRNILCDAKLKAVMGKDEVTMFEMTGLVGKHLS"
     gene            8241..9224
                     /locus_tag="VIN75_22315"
     CDS             8241..9224
                     /locus_tag="VIN75_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY8976966.1"
                     /translation="MILPAIIVVYLACILVVHRRGQVRHSFWGQVFDHSGLLAPVNVF
                     LYAFSAIPNRPFTEVARFPELEALRLNAEAIRAEGLALMAHAKMKSPDQKDDAGFNSF
                     AKSGWKRFYLKWYGDAHPSALRLCPRTTELLRGIPGISAAMFTTLPPGAVLNPHKDPY
                     AGSLRYHLGLVTPNDDRCNITVDGKPYSWRDGEAVIFDETYIHAAENRTDVERLILFC
                     DIERPMRHDFARSFNRWFGRHVVAAASSPNDDSDRIGLINRLFFLSDRAGHYRRRFKA
                     WNRTAYKLTKAGLVAALVAVLVLPTSLRTWHRLHPAGHAVVAATHARTAQD"
     gene            9349..10173
                     /locus_tag="VIN75_22320"
     CDS             9349..10173
                     /locus_tag="VIN75_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEY8976967.1"
                     /translation="MSSDTALAELRLTTRADHDRIERILRLDEAMPLARYAAILSRFD
                     AFLRVWEPRIHAALPERLQSWFRARWRGGLASADVQWLRSVAQVRMPPVDVPAAATLP
                     LTDLPEVLGSLYAFEASALGGRAIAPLLKRSLGLGQGSGASYFHGFGGDTAAMWSNYR
                     VLAALEIGDSPRDTVRACMSARRTFGALIDLFAPLAPAQVPAAPRLAVAQRIAPALLM
                     AHAGPDDEDDEATRPAPLADIHIDLPADGEADAGDTLVELPMDDELDGGDTIRMPL"
     gene            complement(10197..10619)
                     /locus_tag="VIN75_22325"
     CDS             complement(10197..10619)
                     /locus_tag="VIN75_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976968.1"
                     /translation="MRANPSFLLPCGALLLAVAGCATQQSRTEAYEKALSQWQGASEQ
                     TLRARWGTPVSEEPAGHGKWLTYVVNNGATPAPTMSFSIGGFGFGGGGHTSVGGGVGV
                     TTPVGQPTPVTCTTRFLVEDGKVSTWNFDGPGCGSAGG"
     gene            10799..>11169
                     /locus_tag="VIN75_22330"
     CDS             10799..>11169
                     /locus_tag="VIN75_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8976969.1"
                     /translation="MPRVSRAEAESHRQQITEASARLFKERGINGVSVADLMGAAGLT
                     HGGFYGHFESKDALAGEAVAHAFGQSAERWRKRVASQPDDAAGRALIVDTFLSTPGVR
                     EVGLGCPSVALAADVAREPAAA"
BASE COUNT         1515 a         4104 c         3900 g         1650 t
ORIGIN      
        1 tcgtccaggc gcgcaaagcc cgtggaggtg gccagttgca cggtgcgcgg caggccgtct
       61 tcaccgatga acacgcgcac gacggccttg ccggactcgc gcatgcgggc ggagatgcgc
      121 gagtagaccg gcgccggcgg ccaggccgag ctggaagaca ccgaccgcgg ctggatctcg
      181 tacgtcaacg acctgcacaa aggccgcaac gcgggcacgg cgtggcgctg gttcatcgac
      241 gccttcgccg tcgcgtgcct ggtcttcgcc gtcaccggcc tcctgatcct caagatgcac
      301 gcggccaacc ggcccggcac ctggccgctg gtgggcctgg gcgtcgtgct gccggtgctg
      361 ctcgcgctgc tgttcatcca ctgacccgat cccctgccgc cggaaccccg atgctgatcc
      421 gcacctcgct cgccctctcg accgccgtcg cggccgtccc cgccctgtcc gccgaggcca
      481 ccctcaggat ccagcttccg cggctggaca tcgccgaata ccacaagccg tatgtcgtgg
      541 cctggatcga gaaggcgtcc gaccagtcct tcgcgggcaa cgtcgcgctc tggtacgacg
      601 tcaacaagcg caacgacggc ggcgccaggt ggctcaagga catgcgctcg tggtggcgca
      661 agagcggcca cgacgcgccc gccgtcgacg gcatcacggg cgccacgcgc accgcggggg
      721 agcacgcgat caacctgctg gacgccaagg cggtcgcggc gctgccgccg ggccggtacg
      781 aggtggtggt cgaggcggcg cgcgagaacg gcggccgcga ggtgctgcgc gtgccgttcg
      841 agtggccgct gaacaagctc cagtcggcca aggtgcaggg ccagggcgag ctggccgccg
      901 tcgcgatcga cgccaagccg tgaggagcgc ccgcatgcga cacccctgcc ctcgcctcgc
      961 cctgctcgcc ggcggcgcgc tgctgtgcct gtccgcgcag gcccatcgcc cgtggctgct
     1021 gccgtccgcc acgctcgtcg acggtccggc gccggtggtc agcatcgatg cggcgatctc
     1081 ggacgacctg ttcgagttcg actcgttgcc gctgcagctc gagacgctgc gcgtcgtcgc
     1141 accggacggc cgcatcgtcg agcccgatgg ccgcatcgag gcgaagcggc gcatcagctt
     1201 cgacgtcgag ctcgcgcagg aaggcacgta ccgcatcgcc ggcgtgaccg acaccaccgt
     1261 ggcctcgtgg aaggccggcg ggcaggccag gcgctggcgc ggcgccctcg cggcgctcgc
     1321 cagcgcggtg ccggccgacg cgcaggacct gcaggtcacg cgccagctcg cgcgcgccga
     1381 gaccttcgtc agccacgcga cgcccgggcc gccgcccgcc gcgatcaccg gcgagggcct
     1441 ggagctgcag ccgctgacca gcccgaccga cctgagcgtc ggcgacgcga gccgctttcg
     1501 cgtcctgctc gatggcaggc ccttcgcgaa tgccgacgtc acgttgatcc gcggcggcaa
     1561 ccgctatcgc tacaagatgg gcgaactcgc cctgaagacg gacgccgccg gcgagttcgg
     1621 cgtcgcctgg cccgaggccg gccgctggtg gctgggcatc aatcacggcg accgcgcgca
     1681 agggggcgcg gcgcccgcgc gtcgcgagtc ctacagcgcc accttcgagg tgctgcccaa
     1741 gtgagcctgg cgccgcgcgt gctcgtgccc cccgccatcg gcaggacgcc accggcgctt
     1801 ggcggacgcg tccacgagtt cacgggccgg acgatgggca cgacctggag cgtgcggctc
     1861 gtggccgcct cgacgccgtg gcgcgccccg gtgggcgagg cgatccagtc ggcgctcgac
     1921 gaggtcgtcg cccagatgag cacctgggac gcgtcctccg acctgtgccg cttcaacgcc
     1981 gcgccgcccg gcacctggca tgcgctctcc gatcacttcc atgaggtgct cgcctgcgcc
     2041 cgcgacatcg ccgcgcacag cgccggcgcg ttcgatccga ccgccggcgc gctggtcgac
     2101 gcctggggct tcggtcccgc gccgcggcgc ggcgtgccgg acgcggcgac gctcgcgcaa
     2161 gcgtgccgca gcgccggctg gcgccgcatc ggcatcgacc ccgcgcgccg cgcgttgcgc
     2221 cagccgggcg gcctggcgtt ggacctgtcg gcgatcgcca agggcttcgg cgtcgatctc
     2281 gtggcccgcc ggctgcaggc gcagggcatc gccgaccacc tggtggaggt gggcggcgag
     2341 ctgcggggca cgggcgtcaa gcccgacggc caaccctggt gggtgcagct cgaacagccc
     2401 gacggcgatg gaaccggcga acgcacgctg ctcgcgctgc acggcctgtc ggtggcgacc
     2461 tccggcgact accgccgctg gttcgagcac gagggcgtgc gctacagcca caccatcgac
     2521 ccgcgcgacg gccagccgat ccgccacggg ctggcctcgg tcaccgtgat ccacgcggac
     2581 tgcatgcggg ccgacgcctg gtcgaccgcg ttgaacgtgc tggggcccga tgccggcctc
     2641 gcactcgctg atcgcctggg cctcgccgcg cgattcctcg tgcgcgatgg cgagggcttc
     2701 gtcgaacggc gcagcgccgc cttcgacgcg ctgctgcaat gaccgcgttc ggcgtcgacg
     2761 gcgcgcgcct gctgggcgcc gccgccgtgg tcgtgtcgta cgcggcgctg tgcctggcgg
     2821 tcgtgtggaa cgaacggcgc aaggccgcga acgcggcggt gcgaacgaag gcgctcgcgg
     2881 gcggcacgaa cgccaagccc ctgttgatcg tccacgccag ccagaccggc gccgccgaac
     2941 ggctcgcgtg gcaggcggcg cacgcgctgc gcgctgccgc gatgccgctg cgcgtggcgg
     3001 ccatcggcac gctgacgacg ggcgagcttg cgtccgcgtc ggccgtgctg ttcatcgcca
     3061 gtacctgtgg cgaaggcgac gcccccgacg ccgcgctgcc gttcgtgcgc cgcgtgatgg
     3121 acatcgccgg cacgccctcg ctggccggct tgcgctacgg cctgctcgcg ctcggcgatc
     3181 gcgagttcgc gcacttctgc gccttcggcc gtcgtctcga cgactggctg cgcgagcacg
     3241 gtgcccggcc gctgttcgag cgcatcgatg tcgacgcgat ggacgcttgc gcgctcggac
     3301 gttggaagca cgagctcggg cggctgggca gcgtcggcga cgcgctggcc tggcacgaac
     3361 cggcgcgcga gacctggcgg ctcgcgcggc ggacgcacgt caccccgggc agcctgggca
     3421 accccgtgtt ccacctcgag ctcgagcccc ccgccggcgc cgcgccccac tgggagcccg
     3481 gcgacctgct gcaggtccgc gtgccgcacg cgcccgatgc gccccgtccg tactcgatcg
     3541 cctcgattcc cgccgacggc cgcgcccacc tgctggtgcg acagcaacgc cgcggcgacg
     3601 ggcaaccggg actggcctcg ggctggctga cggcggacgc gccgatcggc ggcgacgtcg
     3661 tgctcgacct gttgccgcat cgcaatttcc gcctgggcga caacgcccgg cgcccgctga
     3721 tcctggtggg caacggcacc ggcatcgcgg gcctgcgggc gcacctgcgc gcgcgggcgt
     3781 cacgcgcggg cgccggcccg gccacgcggc cgcgcaactg gctcgtcttc ggcgagcgcc
     3841 aggcggcgca cgacttccac ttccgcgccg acatccaagc ctggcaggcc gacggcacgc
     3901 tggatcgcgt cgacctcgcg ttctcgcgcg accaggcgcc gcgctgctac gtgcaggacc
     3961 gcctgcgcgc ccagctcgcg ctggtgcgcc agtggctgga cgacggcgca gccgtctatg
     4021 tctgcgggag cctgcgcggc atggccggcg gggtcgagga ggcgctggtc gagatcgtcg
     4081 gcgatgagcg cctgcaggcc ctgaaggccg aggggcgcta ccggcgggat gtctactgaa
     4141 cggcgcggga cgattccgag cgacgtcccg aggcgcgtgg tcctgcgact gcgcttggct
     4201 ccgcgcggga tgacggcctg gcgcttcgcg ctcgacattc cggtgcagcg tcgaggagcg
     4261 atgccgtcat cctgcgcgca gtcgcaggat ccactccgca gccgcggcaa tggagcaagg
     4321 cggccgcgac accggcgtca gacctccgac cacgcccgca cgaggttgtg atagtgcccg
     4381 gtcaacccga gcacggcctc gttgtcgccc acctgcgcgc gcagcgcctg gatgttctgg
     4441 tcgagctcga acagcgcacg ccgccgggcc gtgtcgcgca ccaggctctg cacccagaag
     4501 aacgacgcga cgcgcgcgcc gcgcgtgacc gggtgcacgc gatgcaggct gctcgacggg
     4561 tacagcacca ggtcgcccgc cggcagcttg acttcgtgcg tgccgtaggt gtcgacgatc
     4621 tccagctcgc cgccgtcgta ctcgtgcggg ccggacagga acagcgtgca cgacaggtcg
     4681 gtgcgcatct gcaacgtcgt gccgggcacc gagcggatcg cgccgtccac gtgcaggccg
     4741 tagtgctcgc cgccctcgta gcagttgaac aggggcggca tgtggcgcag cggcagcgcg
     4801 gcggacaggt acagcgggtt ggccagcaac gccttgagca cgacgcgccc gagctcctgg
     4861 ccggtgggcg agaactcgtc gagctggcgg ttgcgcttga cccgcgcgcc ctgctcgccg
     4921 acggtggcgc ggccgtcgac ccactcggcg tcggcgaggc gccgccgcag gtcggcgacc
     4981 tggtcggaca ggaggacgtc gggcacgtgc agcagcatgg tgatgagccg gttcgtcgaa
     5041 gggcgttggt cagaatttcg cgtcgagcga caccagcgcg ttgcgcgacg cgcccggcgt
     5101 atagcgcgag ccgccgttgt tgagcgactg catggtcttg cggtcgaaca ggttgtagac
     5161 gttgagctgc acctgccagt ggcgcgaaag ctcgtacgac gccatcgcgt cgcccaccca
     5221 gtacgccggc gcgttcagca ggttggcggt cggcgccgcg gtgttgctgc tgcgcgcgga
     5281 cgtgtccgcg tagcgcatgc cgccgcccag cgtgacgccc agcggcagcc tggccgtgac
     5341 ccacgacgtg aaggtgttcc gcggcgagaa cacgatcacc ccgccctgct gcgccggcgc
     5401 gccgcgcacg atggtcgagt ccatgttcga gtagccgagg ctcacgctca ggtccttcgc
     5461 gaccttgccc accagcccca gctccacgcc ctgcacgcgg cgcttgccga cctgggcgta
     5521 ggtcggcacc gtggcgccgt cggacacgag ttcattgctg ttctcgctgc gatacacggc
     5581 agccgtcgcc agcaggtcgc cgcccagcag gtcccacttc gtgccgaact cgatgttcgt
     5641 gccttcctgc ggcttcaggc cgggctgggc cgcgctgctc gcctggctgg acagcgcgaa
     5701 gttcgtgccc cctggcgggt tgcgcgaatt cgagtacgtg acgtacacgc tgccggtctt
     5761 cgcgggcttg aagacggcgc cgaccttcca gctccacaac gtgtcggtca ggcccagcgg
     5821 cgcgttgttg gccagcgccg cgggcgtcgc gccggaggca gccgtgtacg tcgcgccgct
     5881 cgtgtcggtg tggaaccggt cgacgcgcgc gccggcattg agcagcagcg cgtccgtgac
     5941 gtccagcgtc tcgaacgcgt aggccgacac ggtcagcgtg ctgccgatgg tctgcgtgcc
     6001 cttgtacgac ggcatcgcca tcgcgtcgtc cgggttcggc gcatacaggc tcgcgctgcc
     6061 ggccaccgtg acgggtgtcg ccgtcctctg gcgctcccag ctcagctcga agccgctgga
     6121 cagcgcgctg cccaggccga aggtctcgaa ctgcgtcgac aggttggcct ggtgcgccag
     6181 gatctcgttg tcctggtggc ggccctggcg cgagcgcgcc accgtgtagt ccgacgggtc
     6241 ggccgccgtc gcggtcacgg cgttgacgcc cgtgaccacc acgtcctgca ccgtgcggcc
     6301 gatgcgcgtc gtgttgcgca gcacgacgcc cttggcgagg tccgtctcga gcatcgaggt
     6361 gaacatgtcc accttgacgt ggtcgaagtc gctgcgcagg ccgtagaact tgcccgggtc
     6421 gacggccggg ccgggctgct gcgccggcgc cgtcgcggtg gccgcggcga agacgtagcc
     6481 gtccaggccg aaggtggaca cgccgccgtc gggccggttg tcctgctgga tgtgcagcag
     6541 gctgatcgtc tcgcgcgtgc tcgagccgag gccgaacgtg gccgtgggcg cgaggcccca
     6601 gcgcttctgg cgcacctcgt cgcggcccgg cgtcccgtcg tcctgcgcca tcacgttcag
     6661 gcgcacggcg ctgcccggca cggacgccag cggcgtgttg aagtccgcga ccaggcgctt
     6721 gcggtcggcc gtgccggcga cgaggctgcc ggtggcgaac ccgccgtcgg cctgcgccgt
     6781 cttcgacacg aggttgatgt aggccgacga cgcgccgcgt ccgatgtccg agcccgacgg
     6841 gcctttcacc acttcgacct ggtcgatgtc gaacacgtcg cgcgacacgg cgccgaggtc
     6901 gcggatgccg tcgacgaaga tgctctgcga agtgtcgaag ccgcgcaggt tgatcgagtc
     6961 gcccgtctgc gtgctgccgt tctcgcccag ttgcatcgtc acgcccggcg tgttgcgcag
     7021 cgcctccgac agcgaggtgg cggcctgctg ctgcagcaac tccttcctga tgacggtgat
     7081 cgtctgcggc gtgtcgacga tgggctgcgt gaacttgggc gacgagacga cgtcggcctt
     7141 gtagtcgttc tccggcgccg ccgcctgcac cttgaccgtg ggcagcgtgg tctcgccgcg
     7201 gggctgcgag gcggaagcgg ccggcacggc ggccgactgg gccagcacca accccggaag
     7261 cgcgagcgcg gtggcggcga gcaggacggt cgagtggtgg cgcggttgac gggcggccgc
     7321 gtgcttgcgg ctctggatgg ggcgcatgga tctgtccttc gaacgttggc gggggacgtt
     7381 tcggctggcg tccacggccc tcgtgtcgga cgagatcctc ctggagatgc tggcgagacc
     7441 cggtgaaccg gatcgatctc aaatgctaat gattctcatt aacaaagtca acgtcgagtg
     7501 acggggcggc catcgacgac gcctagcaca ggtcgatgcg gcccgcgcgc gacaccgggg
     7561 cggcgcggag ggcgtatgct cgcgcccttt cccgttctcg aagaggattc ccccgatggc
     7621 taccgcagca accgaaaagt cttccgcctt catgaaggcc ctcaagccga gccccgcgct
     7681 cgccgccatc gtcggctccg agccgctgcc gcgcaccgaa gtcaccaagc gcctgtggga
     7741 ctacatcaag gcccacaacc tgcagaaccc ggccaacaag cgcaacatcc tgtgcgacgc
     7801 caagctcaag gccgtcatgg gcaaggacga agtgacgatg ttcgagatga ccggcctggt
     7861 cggcaagcac ctgagctgat cccgcatcgc gccacgcgcc cgcgcgcgtg acgccaccct
     7921 gcgcagcgcg gccacgcccg cgcctgcgcg cgcccttccg gcgcccctgg ccgccttgtc
     7981 ctggccggcc ttgattcccc gcaccgctcc acggctttct ccgagctgaa tatccgccgg
     8041 gtcagggagt atcaggcacg tccattgctg caacttccgc ttgggcccga tgaatatcac
     8101 ggcttgcctt gtcggattct taccggcggc aattccctgc cgacctgatg ccattcgatg
     8161 cggcgtcgag ccaacagtcg ccgatctgac cgatgctgcc gcggaactcc cgtccggcat
     8221 tgttctcaga aaatctcccg atgatcctcc ccgccatcat cgtcgtgtat ctcgcctgca
     8281 tattggtggt gcaccggcgc ggccaggtgc gccacagttt ctggggccag gtgttcgacc
     8341 attccggcct gctcgcgccg gtcaacgtct tcctgtacgc gttctcggcc atccccaacc
     8401 ggccgttcac cgaggtggcg cgctttccgg agctcgaggc gctgcgcctg aacgccgagg
     8461 ccatccgcgc ggaaggactg gccctgatgg cccacgcgaa gatgaagtca ccggaccaga
     8521 aggacgacgc ggggttcaac tcgttcgcga agtcgggctg gaagcgcttc tacctgaagt
     8581 ggtacggcga cgcccaccct tcggcgctgc gcctgtgccc gcgcaccacg gaactgctgc
     8641 gcggtattcc cgggatcagc gccgcgatgt tcacgacgct gccgccgggc gccgtgctca
     8701 atccccacaa ggatccgtat gccggttcgc tgcgatacca cctgggcctg gtcacgccca
     8761 atgacgaccg ctgcaacatc acggtcgacg gaaaacccta cagctggcgc gacggcgagg
     8821 ctgtgatatt cgacgaaacc tacatccacg ccgcggagaa tcgcaccgac gtggagcggc
     8881 tgatactgtt ctgcgacatc gagcggccga tgcgccacga tttcgcgcgc agtttcaacc
     8941 gctggttcgg gcgacatgtc gtggcggcgg ccagttcccc caacgatgat tccgaccgca
     9001 tcggcctgat caatcgcctg ttcttcctgt cggaccgcgc cggccactac cgccgccgct
     9061 tcaaggcctg gaaccgcacg gcctacaagc tgaccaaggc gggcctcgtc gcggcgctgg
     9121 tggccgtcct cgtgctgccg acgtcgctgc gcacgtggca tcgcctgcat ccggccggcc
     9181 acgcggtggt cgccgcgacg cacgcgcgta ccgcgcagga ctgagcaatc gttcatgccg
     9241 cgaacgtttg cgcgggccgc cgcgccgcga ccgggcgctc gcgacgggtc cgatccgcta
     9301 cgatccggac cgagaccagc cttgcccgtc cgctgagacg tcccttcctt gagctccgac
     9361 accgccctcg ccgaactgcg cctgacgacc cgcgccgacc acgaccgcat cgaacgcatc
     9421 ctgcggctgg acgaggccat gcccctggcg cgctacgccg cgatcctgtc gcggttcgac
     9481 gcgttcctgc gcgtctggga gccgcgcatc cacgccgcgc tgcccgagcg gctgcagtcg
     9541 tggttccgcg cgcgctggcg cggcggcttg gccagcgcgg acgtccaatg gcttcgcagc
     9601 gtcgcgcagg tccgcatgcc gccggtggac gtcccggccg ccgccacgct gccgctgacc
     9661 gacctgcccg aggtgctcgg ctcgctgtac gccttcgaag cttctgcgtt gggcggccgc
     9721 gccatcgcgc cgctgctcaa gcgctcgctg gggctgggcc agggcagcgg cgccagctac
     9781 ttccacggct tcggcggcga caccgccgcc atgtggagca actaccgcgt gctggccgcg
     9841 ctcgagatcg gcgactcgcc gcgcgacacc gtgcgcgcct gcatgagcgc gcgccgcacc
     9901 ttcggcgcgc tgatcgacct gttcgccccg ctcgcgcccg cccaggtgcc ggccgcgccg
     9961 cgcctggccg tcgcgcagcg catcgccccc gcgctgctga tggcccacgc cggcccggac
    10021 gacgaagacg acgaggcgac gcgtcccgcg ccgctggccg acatccacat cgacctgccc
    10081 gccgacggcg aggccgacgc cggcgacacg ctggtcgaac tgccgatgga cgacgagctc
    10141 gacggcggcg acaccatccg catgccgctc tgaggcgcgc gacgcgcctg ccctggttat
    10201 ccgccggcgc tcccgcaacc cggcccgtcg aagttccacg tgctgacctt gccgtcctcg
    10261 acgagaaagc gcgtcgtgca cgtcacgggc gtcggctggc cgacgggggt ggtcacgccc
    10321 acgccgccgc ccaccgaggt atggccgccg ccgccgaacc cgaagccgcc gatgctgaag
    10381 ctcatcgtcg gcgccggcgt cgcgccgttg ttgaccacgt acgtgagcca cttgccgtgg
    10441 cccgcaggct cctcggacac gggcgtgccc cagcgcgcgc gcagcgtctg ctcggaggcg
    10501 ccctgccact ggctcagcgc cttctcgtag gcctcggtgc gcgattgttg cgtcgcgcag
    10561 cccgcgacgg ccagcagcag cgcgccgcag ggcagcagga aacttggatt ggcgcgcatg
    10621 gcgggctcct gtggcgatgg tcctggcgtg atgatgcgcc atccggggca agccggccgc
    10681 ccgtggctcg cggccgcgcc cgcgatgccc gtcccgcggg caggtttatt catgatgctg
    10741 gacatccatt tatcatgatg cctatacttt aaaaaccgca tccacccccg gagcccccat
    10801 gccacgcgtc tcccgcgccg aggccgaaag ccaccgccag cagatcaccg aggcgtccgc
    10861 gcgcctgttc aaggaacgcg gcatcaacgg cgtcagcgtg gccgacctga tgggggccgc
    10921 cggcctcacg cacggcggct tctacgggca cttcgaatcc aaggacgcgc tggccggcga
    10981 ggcggtcgcc cacgcgttcg ggcagtcggc cgagcgctgg cgcaagcgcg tcgcgagcca
    11041 gcccgacgac gccgccgggc gcgcgctgat cgtcgacacg ttcctgtcga cgcccggcgt
    11101 gagggaggtc gggctgggct gcccgtcggt cgcgctcgcg gccgacgtcg cgcgcgaacc
    11161 cgcggcggc
//
LOCUS       DATCRO010000818         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1010444, whole genome shotgun sequence.
ACCESSION   DATCRO010000818 DATCRO010000000
VERSION     DATCRO010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010444"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..644
                     /locus_tag="VIN75_22335"
     CDS             69..644
                     /locus_tag="VIN75_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8976970.1"
                     /translation="MASEPINLTNQFLIAMPGMVDEAFAGAVVYLCEHSEKGALGLMI
                     NKPVDIRLRNLFEKVDLTLASDDLGEQPVFYGGPVQTERGFVLHERIEGSTQYNSTLS
                     VPGGLEMTTSKDVLEAMAVGNGPRRVLVTLGYSGWATGQLEDELGRNGWLNVDAAPEI
                     IFDTPIEQRYERALALLGIDPRMLSQEAGHA"
     gene            656..1060
                     /gene="ruvX"
                     /locus_tag="VIN75_22340"
     CDS             656..1060
                     /gene="ruvX"
                     /locus_tag="VIN75_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8976971.1"
                     /translation="MVDSPARAVRSFLAFDYGTKRVGVATGNTLMKLTQPLRTLALEG
                     DARFKAIEALIKEWAPDALVIGVPFHPDGAEHDNTARARKFGRQLHGRFRLPVHEVDE
                     RYTTVEVESAGASDADSAAAALILEQFFRENE"
     gene            1057..1560
                     /gene="pyrR"
                     /locus_tag="VIN75_22345"
     CDS             1057..1560
                     /gene="pyrR"
                     /locus_tag="VIN75_22345"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY8976972.1"
                     /translation="MTSLSLDAEQLYAELRAGVGRLLTPDTVLVGIWSGGAWLAQRLN
                     ADLQTNRPVGTISSTLHRDDFGERGLGAVEAATELPFEIDGARILLVDDVIASGRTIR
                     AVVNELFDYGRPASVELAVMVDRGGRELPIAPVFAAAKLTLPNHQRLSLAREADGRFT
                     FAVKETT"
     gene            1560..>2444
                     /locus_tag="VIN75_22350"
     CDS             1560..>2444
                     /locus_tag="VIN75_22350"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8976973.1"
                     /translation="MLARRNPQLNAHGELIHLLSIEGLPRAVLTQILDTAGTFLSVND
                     RDVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIAKSSASKGESLLD
                     TIANLSAMHADMFVVRHSESGAPYLIARHCAPHVHVVNAGDGRHAHPTQGLLDMYTIR
                     HYKKSFNELTVAIVGDIVHSRVARSDIHALTTLGVPEIRAVGPKTLVPGDLREMGVRV
                     CHDMREGVKDADVIIMLRLQNERMSGAMLPSAGEYFKSFGLTEDLLAQARPDAIVMHP
                     GPINRGVEIDSRVADGSHS"
BASE COUNT          409 a          871 c          805 g          359 t
ORIGIN      
        1 accggtttgc ggggggagat ctccatggcg gtcgattgtg gcgcccccgc tgccctaaaa
       61 tcgcggcaat ggcctccgaa cccatcaacc tgaccaacca gttcctcatc gccatgcccg
      121 gcatggtcga cgaggcgttc gccggcgcgg tcgtctacct gtgcgagcac agcgagaagg
      181 gcgcgctggg gttgatgatc aacaagccgg tggacatccg cctgcgcaac ctgttcgaga
      241 aggtcgacct gacgctggcc agcgacgacc tcggcgagca acccgtgttc tacggcggcc
      301 cggtgcagac cgagcgcggc ttcgtgctgc acgaacgcat cgagggctcc acgcagtaca
      361 actcgacgct gtcagtgccc ggcggcctcg agatgaccac cagcaaggac gtgctcgagg
      421 ccatggccgt gggcaacggc ccgcggcgag tgctggtcac gctcggctac agcggctggg
      481 ccaccggcca gctggaggac gagctgggcc gcaatggctg gctcaacgtc gatgcggcgc
      541 cggagatcat cttcgacacg ccgatcgagc agcgctatga gcgcgcgctg gccttgctgg
      601 gcatcgaccc gcggatgctc agccaggaag ccgggcacgc atgagcgttt cggccatggt
      661 cgactcgccc gcccgcgccg tccgatcctt cctcgccttc gactacggca cgaaacgcgt
      721 gggcgtggcc acgggcaaca cgctgatgaa gctgacgcaa ccgctgcgca cgctggcgct
      781 cgagggcgac gcgcgcttca aggcgatcga ggccttgatc aaggaatggg cgcccgatgc
      841 gctggtgatc ggcgttccct ttcatccgga cggtgccgag cacgacaata ccgcccgggc
      901 ccgcaagttc ggccgccagc tccacgggcg gttccgcctg cccgtgcacg aggtcgacga
      961 acgctacacc acggtcgagg tggaatcggc cggtgcgagc gatgccgatt ccgcggcggc
     1021 tgccctgatc ctcgaacagt tcttccggga aaacgaatga cttccttatc gctcgatgcg
     1081 gagcagctct acgccgagct gcgcgccggc gtcggccggc tgctgacgcc cgacaccgtg
     1141 ctcgtgggca tctggtcggg cggcgcctgg ctggcccagc gcctgaacgc cgacctgcag
     1201 acgaaccggc cggtcggcac catctccagc acgctgcacc gcgacgactt cggcgagcgc
     1261 ggcctgggcg ccgtcgaagc cgcgaccgag ctgccgttcg agatcgacgg cgcgcgcatc
     1321 ctgctggtcg acgacgtcat cgccagcggc cgcacgatcc gcgcggtcgt caacgagctg
     1381 ttcgactacg gccggccggc atcggtggaa ctggccgtca tggtcgaccg cggcggccgc
     1441 gagttgccga tcgccccggt cttcgccgcc gccaagctca cgctgccgaa ccaccagcgc
     1501 ctgtcgctcg cgcgcgaggc cgacggccgc ttcaccttcg ccgtcaagga gaccacctga
     1561 tgctcgcgcg acgaaatccg cagctcaacg cccacggcga gctgatccac ctgctgtcca
     1621 tcgagggcct gccgcgcgcc gtcctgacgc agatcctgga caccgccggc accttcctct
     1681 cggtcaacga ccgcgacgtc aagaaggtgc cgctgctgcg cggcaagagc gtgttcaacc
     1741 tgttcttcga gaactccacg cgcacgcgca ccacgttcga gatcgcggcc aagcggctgt
     1801 cggcggacgt gatcaacctg gacatcgcca agtcgtcggc gtccaagggc gagagcctgc
     1861 tcgacaccat cgccaacctg tcggccatgc acgccgacat gttcgtcgtg cgccacagcg
     1921 agtcgggcgc gccgtacctg atcgcgcgcc actgcgcccc gcacgtgcac gtggtcaacg
     1981 ccggcgacgg ccgccatgcg cacccgacgc agggcctgct ggacatgtac acgatccggc
     2041 actacaagaa gtcgttcaac gagctgacgg tggccatcgt cggcgacatc gtgcattcgc
     2101 gcgtcgcacg ttccgacatc cacgcgctca ccacgctggg cgtgcccgag atccgcgccg
     2161 tcggccccaa gacgctggtg ccgggcgacc tgcgcgagat gggcgtgcgc gtgtgccacg
     2221 acatgcgcga gggcgtgaag gacgccgacg tcatcatcat gctgcgcctg cagaacgaac
     2281 gcatgagcgg cgcgatgctg cccagcgcgg gcgagtactt caagagcttc ggcctgacgg
     2341 aagacctgct ggcgcaggcc aggcccgacg ccatcgtgat gcacccgggc ccgatcaacc
     2401 gcggcgtgga gatcgactcg cgcgtggccg acggctcgca cagc
//
LOCUS       DATCRO010000819         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_414090, whole genome shotgun sequence.
ACCESSION   DATCRO010000819 DATCRO010000000
VERSION     DATCRO010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414090"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="VIN75_22355"
     CDS             complement(<1..1833)
                     /locus_tag="VIN75_22355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8976974.1"
                     /translation="MLQTQDLKVALDAELGLVKAIDGLTLAIQRGETFALVGESGCGK
                     SMTALALMRLLPENGRITGGDIRLDRDDLLALPEAGMRAVRGGRIGMIFQEPSTSLNP
                     VMKIGAQLVEAIEAHTTLRGAQARQKAIDWLRRVGIPEPERRIDDYPFRMSGGQKQRV
                     MIAMTLATEPDFLVADEPTTALDVTIQAQVLDLLKQLQQEQGLGMLLITHDLAVVKGM
                     AHRVALMYAGQIIEVAKAGDFFANPKHPYARALLRALPDARRRGQPLQAIPGTVPVLT
                     QSFAGCRFAPRCDRVMAHCAGVVPELIEINGREQPGDVHAVRCLLFAPGAASAAPAAA
                     APRVEAPRAPAAPAAAASVPLLQVRNLDVRFPLRTGLFQRGKRFFNAVDDVSFTVGEG
                     ETLALVGESGCGKTTTGKAVVQLLRGVATFEGQALLRGRNLFELSGQALVQARRDIQI
                     IFQDPFASLNPRMRVAEILDEGMVSLQPEVDAAARRRRIEGLVDQVGLRRDALDRYPH
                     EFSGGQRQRIAIARALAVQPKLIVCDEPTSALDVSVQAQILNLLHDLQQDLGVSYLFI
                     THNIGVVEYIADRVAVMRAGRIVEQGDCVSVLEHPQQDYTRALID"
     gene            complement(1838..3307)
                     /locus_tag="VIN75_22360"
     CDS             complement(1838..3307)
                     /locus_tag="VIN75_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976975.1"
                     /translation="MSPKFVLLWTDATTWALILCMIVYAVAVARSPNLKASWRKVFRD
                     APALCSSLILGLCVLVTAADSVHYRNALPPVPGTPPGVVAYDSVTRSGLDWMLSSLIE
                     SREDTYSRPLDYLSYRRDTVSINGQLQRISPRLKHGGAHLSDPDHQWAADLGSRAGTG
                     LALGLALAAACALVLFGLLARARGAGFLETAGSVWRKEGDIPWRAGLWTLVVIATLSG
                     VVVSLMGAYHVFGTDTTGNDVLYQSLKCIRTAFVIGSLSTIATLPLAVVLGIMAGYFR
                     GWVDEVIQYFYTVLSSVPNILLIAACVLMVQVFLDKHPDMFETGVERADVKLALLCAV
                     LGLTGWASLCRLLRAETMKLRELDYVQAANAFGVSATRIMVRHVFPNVAHLMLIVTVL
                     DFSSLILYEAVLSYVGVGVDPSMNSFGGMLNLARKEMSLDPMVWWSFFAAFGFMVTVV
                     LSVNLFADGVRDAFDPRARAFRPRLPVTRKPAAPSPNQG"
     gene            complement(3319..>3898)
                     /locus_tag="VIN75_22365"
     CDS             complement(3319..>3898)
                     /locus_tag="VIN75_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8976976.1"
                     /translation="LLVMFRHTRLDFWGVVTCVLMLSISSLIYIIAGQVMFSRLLKVA
                     PISGYVPGWDVAKFLLLPIVLSLLARLGTEARLYRAMFLEEISKDYVRTARAKGLAET
                     AVLFRHVLRNALLPIVTSAGAYLPYVFLGSLVFESFFGLPGLGAYVIDAITKQDFAVV
                     RTMVFVGSLLYIATFVLIDVVYTWVDPRVRLG"
BASE COUNT          643 a         1337 c         1392 g          526 t
ORIGIN      
        1 gtcgatgagc gcgcgggtgt agtcctgctg cggatgttcc aggaccgaca cacagtcgcc
       61 ctgctcgacg attcgccccg cccgcatcac ggcgacacga tcggcgatgt actcgaccac
      121 gccgatgttg tgcgtgatga aaagatacga gacgccgagg tcctgttgca ggtcgtgcag
      181 caggttcagg atctgcgcct gcaccgacac gtccagcgcc gaggtgggct cgtcgcagac
      241 gatgagcttg ggctggacgg cgagcgcgcg cgcgatcgcg atgcgctggc gctggccgcc
      301 ggagaactcg tgcgggtagc ggtcgagcgc gtcgcgccgc aggcccacct ggtcgaccag
      361 cccctcgatg cgccgccggc gcgccgcggc gtcgacctcg ggctgcagcg agaccatgcc
      421 ttcgtcgagg atctcggcga cgcgcatgcg cgggttcagc gacgcgaacg gatcctggaa
      481 gatgatctgg atgtcgcgcc gcgcctggac cagcgcctgg cccgacagct cgaacaggtt
      541 gcgcccgcgc agcagcgcct ggccctcgaa cgtggcgacg ccgcgcagca gctgcacgac
      601 ggccttgccg gtggtggtct tgccgcagcc cgactcgccc accagcgcga gcgtctcgcc
      661 ctcgccgacc gtgaacgaca cgtcgtccac cgcgttgaag aagcgcttgc cacgctggaa
      721 caggcccgtg cgcaggggga accgcacgtc caggttgcgc acctgcagca gcggcacgga
      781 cgccgcggcc gccggcgccg ccggcgcgcg cggcgcctcg acccggggcg cggcggctgc
      841 cggcgccgcg ctcgccgcac cgggagcgaa caacaggcag cgcacggcgt ggacgtcgcc
      901 gggctgttcg cggccgttga tctcgatcag ttcgggcacg acgcccgcgc agtgcgccat
      961 cacgcggtcg cagcgcggcg cgaagcggca gccggcgaac gactgggtca gcacgggaac
     1021 ggtgccgggt atcgcctgca gcggctggcc gcgccggcgc gcatcgggca gcgcacgcag
     1081 cagcgcccgc gcgtacgggt gcttggggtt ggcgaagaaa tcgccggcct tggcgacctc
     1141 gatgatctgg cccgcgtaca tcagcgccac gcggtgcgcc atgcccttga ccaccgcgag
     1201 gtcgtgcgtg atcagcagca tgcccaggcc ctgctcctgc tgcagctgct tgagcaggtc
     1261 gagcacctgc gcctggatcg tgacgtcgag cgccgtcgtc ggctcgtcgg caaccaggaa
     1321 gtcgggctcg gtggccagcg tcatcgcgat catcacgcgc tgcttctggc cgccgctcat
     1381 gcggaacgga tagtcgtcga tgcggcgctc gggctcgggg atgccgacgc ggcgcagcca
     1441 gtcgatcgcc ttctggcgcg cctgcgcgcc gcgcagcgtc gtgtgcgcct cgatcgcctc
     1501 gacgagctgc gcgccgatct tcatgaccgg gttcaggctg gtcgagggct cctggaagat
     1561 catgccgatg cgcccgccgc gcaccgcgcg catgccggcc tcgggcagcg cgagcaggtc
     1621 gtcgcggtcc aggcggatgt cgccgccggt gatgcggccg ttctcgggca acagccgcat
     1681 cagcgcgagc gccgtcatgc tcttgccgca gcccgattcg ccgacgagcg cgaaggtctc
     1741 gccgcgctgg atggccagcg tcaggccgtc gatggccttg acgaggccga gctcggcgtc
     1801 gagcgccacc ttcaggtctt gggtctggag catggggtca gccttggttc ggcgacgggg
     1861 cggccggctt gcgcgtgacg ggcaggcgcg gacggaacgc gcgggcacgc gggtcgaagg
     1921 cgtcgcgcac gccgtcggcg aacaggttga cggacagcac caccgtcacc atgaagccga
     1981 acgcggcaaa gaagctccac cagaccatcg ggtccaggct catctccttg cgcgcgaggt
     2041 tgagcatgcc gccgaagctg ttcatcgacg ggtcgacgcc cacgccgacg tacgacagca
     2101 cggcctcgta caggatcagc gacgagaagt cgagcacggt gacgatcagc atcaggtgcg
     2161 cgacgttggg gaacacgtgg cgcaccatga tgcgcgtggc gctgacgccg aaggcgttgg
     2221 cggcctgcac gtagtcgagc tcgcgcagct tcatggtttc ggcgcgcagc aggcggcaca
     2281 ggctcgccca gccggtcagg cccagcacgg cgcacagcag cgcgagcttg acgtcggcgc
     2341 gctcgacgcc ggtctcgaac atgtcggggt gcttgtcgag gaacacctgc accatcagca
     2401 cgcacgcggc gatcagcagg atgttgggca ccgacgagag caccgtgtag aagtactgga
     2461 tgacctcgtc gacccagccg cggaaatacc ccgccatgat gcccagcacg acggccagcg
     2521 gcagcgtcgc gatcgtcgac agcgacccga tgacgaatgc cgtgcggatg cacttgagcg
     2581 actggtacag cacgtcgttg cccgtggtgt cggtgccgaa cacgtggtag gcgcccatca
     2641 gcgagacgac gacgccgctg agcgtggcga tgaccaccag cgtccacagg ccggcgcgcc
     2701 aggggatgtc gccttccttg cgccacacgc tgcccgccgt ctccaggaag cccgcgccgc
     2761 gcgcccgcgc cagcaggccg aacagcacca gcgcgcatgc cgcggccagc gccaggccca
     2821 gcgcgagccc ggtgcccgcg cgcgagccca ggtcggcggc ccactggtgg tcggggtcgg
     2881 acaggtgcgc gccgccatgc ttcaggcgcg gcgagatgcg ctgcagctgg ccattgatcg
     2941 acacggtgtc gcggcggtag ctgaggtagt cgagcggccg cgaataggtg tcctcgcgcg
     3001 attcgatcag gctggacagc atccagtcca ggcccgagcg cgtgaccgag tcgtaggcga
     3061 cgacgcccgg cggcgtgccc ggcaccggcg gcagcgcgtt gcggtagtgg acgctgtcgg
     3121 ccgcggtgac cagcacgcac aggcccagga tcagcgacga gcacagcgcc ggggcatcgc
     3181 ggaacacctt gcgccagctg gccttgaggt tgggcgagcg cgccaccgcg acggcgtaga
     3241 cgatcatgca caggatcagc gcccacgtcg tggcgtcggt ccagagcagg acgaacttcg
     3301 gactcatcgt tccgctcctc aacccagtcg cacccgggga tcgacccagg tgtagacgac
     3361 gtcgatcagc acgaacgtcg cgatgtacag cagcgagccg acgaacacca tggtgcgcac
     3421 gacggcgaag tcctgcttgg tgatggcgtc gatcacgtag gcgcccaggc ccggcaggcc
     3481 gaagaagctc tcgaacacga ggctgcccag gaacacgtac ggcaggtagg cgccggcgct
     3541 ggtgacgatg ggcagcagcg cgttgcgcag cacgtggcgg aacagcaccg ccgtctcggc
     3601 caggcccttg gcgcgcgcgg tgcgcacgta gtccttgctg atctcctcga ggaacatcgc
     3661 gcggtaaagg cgcgcctcgg tgcccaggcg cgcgagcagc gacagcacga tgggcagcag
     3721 caggaacttg gccacgtccc agcccggcac gtagcccgag atgggcgcga ccttgagcag
     3781 gcgcgagaac atcacctgcc ccgcgatgat gtagatcagg ctcgatatcg acagcatcag
     3841 cacgcaggtg acgacgcccc agaagtcgag ccgcgtgtgg cggaacatca cgagcagc
//
LOCUS       DATCRO010000820         5373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_729334, whole genome shotgun sequence.
ACCESSION   DATCRO010000820 DATCRO010000000
VERSION     DATCRO010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729334"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIN75_22370"
     CDS             <1..268
                     /locus_tag="VIN75_22370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976977.1"
                     /translation="QPHRADVAKMLVHRRARRRGAGAALMHAAEAEALREGKTLLVLD
                     TADDTAARLYARMGWVAAGTIPRYALLPAGGECATTYFYRSLAA"
     gene            complement(380..1333)
                     /locus_tag="VIN75_22375"
     CDS             complement(380..1333)
                     /locus_tag="VIN75_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY8976978.1"
                     /translation="MSKTPSVAVLDDYQNAALRLADWSVLDGRAAVTVFNDHLADADA
                     VVERLKGFDVLCVMRERTPLPRAMLERLPKLKLIVSTGSRNAAIDVAAAEERGIAVAF
                     TGYTSAPTIEMTWALILAASRHIVAENLAFRRGGWQQGVGVGLAGKTLGVLGLGRVGS
                     AVAKVGAAFGMNVITWSPNMTPERAALAGAEMVTKPELLRQSDVLTIHMVLSPRTRGL
                     LGADDLALMKTSALLVNTSRGPLVDEAALVAALTSRKIALAALDVFDVEPLPEDHPLR
                     SLENVLGTPHIGYVADDLYRTFYGDTVKAVDAWLAARAKTA"
     gene            1493..2047
                     /locus_tag="VIN75_22380"
     CDS             1493..2047
                     /locus_tag="VIN75_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8976979.1"
                     /translation="MSDQASAKRVPLQLDQRALDAAKNNIEEGAITPAYGPWRDDIVK
                     LLNDSLATELVCTMRYKRHYYTAKGMASPAIADEFLVHANEEAEHADMIAERIVQLGG
                     EPDFNPTTLLQRSHADYDESTDLKAMIRANLVAERVAIEAYTQTIRLIGDKDSTTRRI
                     IEHILSQEQEHAEELSDWLDLDGR"
     gene            2131..2400
                     /locus_tag="VIN75_22385"
     CDS             2131..2400
                     /locus_tag="VIN75_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976980.1"
                     /translation="MKAWPILLGGAAACAACCAAPLAAGLAGGTSLLAAGSALFACAA
                     DVAPLAGLLTALGLAIAGATWWRRRARRRMACTPACKECGDGQRA"
     gene            2384..2740
                     /locus_tag="VIN75_22390"
     CDS             2384..2740
                     /locus_tag="VIN75_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976981.1"
                     /translation="MASAPEAPIACTLDSGDLPARLAWIRRVTDDALVAHRRDGATLH
                     LVYDRRARDDLERIVAAERACCAFLDFDLADTGGQVELRIRAPAGLATDAHWLFEQFL
                     PRQPRAACSCGPSACG"
     gene            2750..3217
                     /locus_tag="VIN75_22395"
     CDS             2750..3217
                     /locus_tag="VIN75_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8976982.1"
                     /translation="MSAQLSIGIVARRAGATVPTIRYYEEIGLLPRAARTAAGQRSYG
                     EAAVRRLVFIRRCRDFGFSLEQVRELVGLIDHPERPCTQARDIAAGHLAALRVKLAEL
                     QSLEASLAAFVCSCETECAGGPVADCAILEDLALPAGQSRLAPPAACCGPDAA"
     gene            complement(3293..3706)
                     /locus_tag="VIN75_22400"
     CDS             complement(3293..3706)
                     /locus_tag="VIN75_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976983.1"
                     /translation="MNKSLATLIATAAAAGFSFAAHAQTTDTQPAPGTPGTTTTTTVT
                     TETVETRMVPPPAAGMTRSEAHDAKIQSKADYKARKNIAEANHDLSKADCKINSEGAV
                     ERACKHDARMEEKKEKADAKLIHKQEVQDIDAQTK"
     gene            complement(3829..>5373)
                     /locus_tag="VIN75_22405"
     CDS             complement(3829..>5373)
                     /locus_tag="VIN75_22405"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8976984.1"
                     /translation="SGIAGMPPQAGVLALFAGLLAYGLIGRSRFAIVSATSSSAAVLG
                     AATLSLAGHDAALRAALAAGLVLATGLAFLAAGAMRLGSICSFISKPVLRGFSFGLAL
                     VIIVKQLPKLTGTHPSADAAPAFAWQLLGALPRWNWNALALGLVSLAALKLLAQLPKL
                     PGALLVIAAGIAATHVLALDARGVAVVGRIDLALALPAPPVLSQGQWVSIAELAVALV
                     LLVFAESYGAIRSMALKHGDAIDPNRDLWALGASNLVAGALHGLPVGAGFSATAANEA
                     AGAGSRKAAWIAGLVVLALVALCLPWIERTPEPVLAAIVIHAVSHTLSLAAFRPYFQW
                     RRDRFVVVAAAVAVVALGVLNGLLAGIAISVAMTLRGLSEPRMSELGRHRGGHDYLGL
                     QHADVQRVPGVLILRPEAPLFFANVERMLTEMRARIAAASPHAFVLSLEETPDLDGTT
                     IEALAAFASEQAGAGRATVFARLKDPVLALLQEAMAGQRETLLEAGSVDDAVSSLKAA
                     AATAWP"
BASE COUNT          778 a         1991 c         1873 g          731 t
ORIGIN      
        1 ccagccgcac cgtgcggacg tcgccaagat gctggtgcac cgacgcgcgc gccgccgtgg
       61 cgccggcgcc gcgctgatgc atgccgccga ggccgaggcg ctgcgtgaag gcaagacgct
      121 gctggtgctc gacaccgccg acgacaccgc cgcgcgcctg tacgcccgca tgggctgggt
      181 ggcggccggc acgatcccgc gctatgcgct gctgcccgcg ggcggcgaat gcgcgacgac
      241 gtacttctat cgctcgctgg cggcctgagg cgccgacgcc atcgacgccg ccgggcagtc
      301 cggcagcgtc acgcgtcggg tttgcacgcc ctgtacggcc gcgacgactg cacgcgcccg
      361 gacgcgcgac ggccgcgcat caggcggtct tggcgcgcgc ggccagccag gcgtcgacgg
      421 ccttgacggt gtcgccgtag aacgtgcggt acaggtcgtc ggcgacgtag ccgatgtgcg
      481 gcgtgcccag cacgttctcc agagagcgca gcggatggtc ctcgggcagc ggctcgacat
      541 cgaacacgtc cagtgccgcc agcgcgatct tgcgcgacgt gagcgccgcc accagcgccg
      601 cctcgtcgac cagcggcccg cgcgacgtgt tgaccagcag cgcgctcgtc ttcatcagcg
      661 cgaggtcgtc ggcgcccagc aggccgcgcg tcctggggct cagcaccatg tggatcgtca
      721 ggacgtcgga ctggcgcagc agctcgggct tggtcaccat ctcggcgccg gccagcgcgg
      781 cgcgctcggg cgtcatgttg ggactccagg tgatcacgtt catgccgaag gccgcgccga
      841 ccttggccac cgcgctgccc acgcgcccca gcccgagcac gcccagcgtc ttgccggcca
      901 ggcccacgcc cacgccctgc tgccagccgc cgcgccggaa cgccaggttc tcggccacga
      961 tgtggcggct ggccgccagg atcagcgccc aggtcatctc gatggtcggc gccgaggtgt
     1021 agccggtgaa ggcgacggcg atgccgcgct cctcggcggc cgccacgtcg atggccgcgt
     1081 tgcgcgaccc cgtcgacacg atcagcttca gcttcggcag ccgctccagc atcgcgcgcg
     1141 gcagcggcgt gcgctcgcgc atgacgcaca gcacgtcgaa gcccttcagg cgctcgacga
     1201 cggcgtcggc atcggccagg tggtcgttga acacggtgac cgcggcgcgg ccgtccagca
     1261 ccgaccagtc ggccaggcgc agcgcggcgt tctggtagtc gtccagcacc gcgacgctgg
     1321 gggtcttgct catgggagtg ctcctgcctt gggttgcggc cactgtagac aaatccgcgc
     1381 ccgcatgcgt ccccacggtc ttgcgccatt tcgccaaggg cggcggaccg cgtgcccggc
     1441 atcccgattg ccgtggcgcc tgcgttcaac ctcacactcg agaaggcccg ccatgtccga
     1501 ccaagccagc gccaagcgcg tccccctgca gctcgatcaa agagccctgg atgccgcgaa
     1561 gaacaacatc gaggaaggcg ccatcacgcc cgcctacggt ccgtggcgcg acgacatcgt
     1621 caagctgctc aacgactcgc tcgccaccga gctggtgtgc acgatgcgct acaagcgcca
     1681 ttactacacc gccaagggca tggcctcgcc cgccatcgcc gacgagttcc tcgtgcatgc
     1741 caacgaggag gccgaacacg ccgacatgat cgccgagcgc atcgtgcagc tgggcggcga
     1801 acccgacttc aacccgacca ccctgctgca gcgcagccac gccgactacg acgaatcgac
     1861 ggacctgaag gccatgatcc gcgccaacct ggtggccgag cgcgtggcca tcgaggccta
     1921 cacgcagacg atccgcctga tcggcgacaa ggattcgacg acgcgccgca tcatcgagca
     1981 catcctgtcg caggagcagg agcatgcgga ggagttgagc gactggctgg acctcgacgg
     2041 caggtaggcc gcgaccggcc cttgaccctc aagcggcttg agctcctatc gtcccggcca
     2101 gggcgtcgtg ccctgcatcg gagacgagac atgaaagcct ggcccatcct cctcggcggg
     2161 gccgcggcct gcgccgcgtg ctgcgccgcg ccgctggcgg ccggcctggc cggcggcacg
     2221 tcgctgcttg cggcgggatc ggccctgttc gcgtgcgcgg ccgatgtcgc gccgctcgcg
     2281 gggctgctca cggcgctggg cctggccatc gccggggcca cgtggtggcg ccgccgcgcg
     2341 cggcgccgta tggcatgcac gccggcatgc aaggagtgcg gcgatggcca gcgcgcctga
     2401 agcgcccatc gcctgcacgc tggactccgg cgacctgccc gcgcgcctgg cctggatccg
     2461 ccgcgtgacg gacgacgcgc tcgtcgcgca tcgccgggac ggcgcgacgc tgcacctggt
     2521 ctacgaccgg cgggctcgcg acgacctcga gcgcatcgtc gcggccgagc gcgcatgctg
     2581 cgcattcctg gacttcgacc tggcggacac gggtggccag gtcgaactgc gcattcgcgc
     2641 gccggcaggc ctggccacgg acgcgcattg gctcttcgag cagttcctgc cgaggcaacc
     2701 tcgagccgcg tgcagttgcg gtccgagcgc ctgcggctag catcgcgtca tgagcgccca
     2761 actgtccatc ggcatcgtcg cccggcgcgc gggcgccacg gtgcccacca tccgctacta
     2821 cgaggagatc ggcctgctgc cgcgggccgc acgcacggcc gcgggccagc gcagctatgg
     2881 cgaggccgcg gtgcggcggc tggtgttcat ccgccgctgt cgcgacttcg gcttctcgct
     2941 ggagcaggtg cgcgagctcg tcggtttgat cgaccatccc gaacgcccct gcacgcaggc
     3001 gcgcgacatc gccgccggcc atctcgccgc gctgcgcgtc aagctcgccg aactgcaatc
     3061 gctggaggcg agcctggcgg ccttcgtgtg cagctgcgag acggaatgcg cgggcggccc
     3121 ggtggccgac tgcgcgatcc tcgaggacct ggcgctgccg gcgggccagt cccgcctcgc
     3181 gccgccggcg gcctgctgcg ggccggacgc cgcatgaaca aaggccccgc ggaccgtgaa
     3241 gtccgtgggg cctcgcccag gagcgcgggc atcggaaccg cgctggcggg gcttacttgg
     3301 tctgcgcgtc gatgtcctgc acttcctgct tgtggatcag cttggcgtcc gccttctcct
     3361 tcttctcctc catgcgggcg tcgtgcttgc acgcgcgctc caccgcgcct tcggagttga
     3421 tcttgcagtc cgccttggac aggtcgtggt tggcctcggc aatgttcttg cgcgccttgt
     3481 agtcggcctt cgactggatc ttggcgtcat gcgcttccga gcgcgtcatg ccggcggccg
     3541 gcggcggcac catcctggtc tccaccgtct cggtcgtcac cgtcgtggtg gtcgtggtgc
     3601 ccggcgtgcc gggggcgggc tgggtgtcgg tcgtctgcgc atgcgcggcg aagctgaagc
     3661 cggcggcggc agcggtggcg atgagcgtgg cgagagactt gttcattgct gttctcctgt
     3721 tgcttggaac aaccctggac gggcaatcga gggtcccgcc agctcatgcg tggaagcatc
     3781 gagaaacaac tccgcatggc atgcacggca cgcgtgcacg agcgaacatc agggccacgc
     3841 agtggccgcg gcggccttga gcgacgacac cgcgtcgtcg acgctgccgg cctccagcag
     3901 cgtctcgcgc tggccggcca tcgcctcctg cagcagtgcc agcaccggat ccttcaggcg
     3961 cgcgaacacc gtcgcgcggc ccgcgccggc ctgttcgctc gcgaatgccg ccagcgcctc
     4021 gatggtggtg ccgtccaggt cgggcgtctc ctccaggctc agcacgaacg cgtgcggcga
     4081 cgcggcggcg atgcgcgcgc gcatctcggt cagcatgcgc tcgacgttcg cgaagaacag
     4141 cggtgcctcg ggccgcagga tcagcacgcc gggcacgcgc tgcacgtcgg cgtgctgcag
     4201 gccgaggtag tcgtgcccgc cgcgatggcg gcccagttcg ctcatgcgcg gctccgacaa
     4261 cccgcgcagc gtcatcgcca cgctgatcgc gatgccggcg agcaggccgt tcagcacgcc
     4321 cagcgccacg accgcgacgg ccgccgcgac gacgacgaag cggtcgcgcc gccattggaa
     4381 gtaggggcgg aacgcggcca gcgacagcgt gtgcgacacc gcgtggatga cgatggccgc
     4441 cagcacgggc tcgggcgtgc gctcgatcca cggcaggcag agcgccacca gcgccagcac
     4501 cacgagaccg gcgatccacg ccgccttgcg gctgccggca cccgcggcct cgttggccgc
     4561 cgtcgccgag aagcccgcgc ccacgggcag gccatggagc gcgcccgcca cgagattgga
     4621 cgcccccagc gcccacaggt cgcggttcgg atcgatcgcg tcgccgtgct tgagcgccat
     4681 cgagcggatc gcgccgtagc tctcggcgaa caccagcagc accagcgcga cggccagctc
     4741 cgcgatgctg acccactgcc cctgcgacag caccggcggc gcgggcagcg ccagcgcgag
     4801 gtcgatgcgg ccgacgaccg cgacgccgcg cgcgtccagc gccagcacgt gcgtggccgc
     4861 gatgcccgcc gcgatcacca gcaacgcccc gggcagcttg ggcaattgcg ccagcagctt
     4921 cagcgcggcc agcgacacga ggcccagcgc gagcgcgttc cagttccatc gcggcagcgc
     4981 gcccagcagc tgccatgcga acgccggggc ggcgtcggcc gacggatgcg tgccggtcag
     5041 tttcggcaac tgcttgacga tgatcaccag cgcgaggccg aaagagaacc cccgcagcac
     5101 cggcttggag atgaagctgc agatcgagcc caggcgcatc gcacccgcag ccaggaacgc
     5161 caggcccgtg gccagcacca ggccggccgc gagcgcggcg cgcagcgcgg cgtcgtgccc
     5221 cgcgagcgac aacgtcgcgg cgcccagcac cgcggccgac gacgacgtcg ccgacacgat
     5281 cgcgaagcgc gagcgcccga tcagcccata cgcgagcagc cccgcgaaca gggccagcac
     5341 gccggcctgc ggcggcatgc ccgcgatgcc cga
//
LOCUS       DATCRO010000821         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_470560, whole genome shotgun sequence.
ACCESSION   DATCRO010000821 DATCRO010000000
VERSION     DATCRO010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470560"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..975)
                     /gene="corA"
                     /locus_tag="VIN75_22410"
     CDS             complement(7..975)
                     /gene="corA"
                     /locus_tag="VIN75_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY8976985.1"
                     /translation="MLINCVAYENGSKLADIPIAEISDYLARPGCFVWVAMKDTSDEE
                     LLTMQKEFGLHELAVEDALHGHQRPKVEEYGDTLFAVLHTVELTPTDELTTGEVDIFV
                     GSNFVLSVRNRSRQNLLQVRERSEREPHMLRHGPAFVFYALMDAVVDRYFPIIDRLET
                     ELEQVEDQMFEPGTSKANIQRLYELKREIGTVRHAVTPLIDAVHKLFSGRVPSVCEGN
                     REYFRDVFDHLLRMQGSLDNLRDTIGTAIQVNLANVAIEESVVNKRLAAWAGIFAVIS
                     AFAGIWGMNFKNMPELEWQWGYPLALATITIVCVILYRQFKKAGWL"
     gene            1136..3019
                     /locus_tag="VIN75_22415"
     CDS             1136..3019
                     /locus_tag="VIN75_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8976986.1"
                     /translation="MGKQQHGAGLAAMTLGALGVVYGDIGTSPLYTLHEIFVPVSGVG
                     VPLDAQHIVGAVSTIFWGLMLVVTLKYVILILRADNNGEGGAMALTALAAHAAGGTPQ
                     RRTLIMLVGMFGATLFFGDSVITPAVTVMGAVEGLEVVTPALQAYIVPLSVLILVALF
                     SAQRFGTGAFGKAFGPLLVVWFGLLAVVGALHISQAPEILYALNPLYAVEFLLDRGWH
                     VLAALGAIVLALTGAEALYADMGHFGKTPIRLAWSALVLPALALNYMGQGALLIHDAS
                     AIENPFFRLFPSTWVLPMVAFAAVAAVIASQAVISGAFSMTKQAIQLGFLPRMEVVYT
                     SVKEAGQIYLPRVNWVLMVAVVCAVIGFGSVSKLTAAYGIAVTVTMLIDTVLTYFVVR
                     YGWGFPLWIALGATGFFAIIDGLLVAACSLKFLHGGWFPLLLALAMFAIMSTWKRGRE
                     LLLESIRRDDPDLIPFITALAEDDITRTPRTAVFTVANVGTVPQALLHNLKHNQVLHE
                     TNVILTVKFHEVPWIPFADRVQVEPLARGFWRVTVNYGFKNTPDIPQALTLCRAHGLD
                     VDEFTTSYFLSREVVVPTRGDGMAHWRERLFAAMTRNAGSVVEFFRLPNNAVIELGTR
                     VQI"
     gene            3340..>4004
                     /locus_tag="VIN75_22420"
     CDS             3340..>4004
                     /locus_tag="VIN75_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976987.1"
                     /translation="MRLAPGTIHFLTLGVASLAMLSACGGGGSDAAPTPPAPKPTATH
                     LTGSAAVGAPMTQGTLRVIDATGAVVAHDIPINADGTYDAGVLTGTGPWRIEACGYTG
                     ANYGCIYSVAQAAGTANVTPLTSAMITLATGQKADAVMSAASAPGASALGAAQDQLRS
                     GLAGTLSDSGVPPDIDFTTGALQAGSRSGYDRVLDAVDVTTGTDNGAFVQVTPRMGDG
                     NLY"
BASE COUNT          571 a         1398 c         1365 g          670 t
ORIGIN      
        1 ggccgtctat aaccagccgg ccttcttgaa ctggcgatag aggatcacgc acacgatcgt
       61 gatcgtcgcc agcgccagcg ggtagcccca ctgccactcg agctcgggca tgttcttgaa
      121 gttcatgccc cagatgccgg cgaacgcgct gatgacggcg aagatgccgg cccaggccgc
      181 gaggcgcttg ttgacgacgg attcctcgat cgccacgttg gccaggttga cctggatggc
      241 ggtgccgatg gtgtcgcgca ggttgtccag cgagccctgc atgcgcagca ggtggtcgaa
      301 cacgtcgcgg aagtactcgc ggttgccctc gcagacggac ggcacgcggc cgctgaacag
      361 cttgtgcacg gcgtcgatca gcggcgtcac cgcgtggcgc acggtgccga tctcgcgctt
      421 gagctcgtac aggcgctgga tgttggcctt ggaggtgccg ggctcgaaca tctggtcctc
      481 gacctgctcg agctccgtct cgaggcggtc gatgatgggg aagtagcggt cgacgaccgc
      541 gtccatcagc gcgtagaaca cgaacgccgg gccgtggcgc agcatgtgcg gctcgcgctc
      601 ggatcgctcg cgcacctgca gcaggttctg gcggctgcgg ttgcgcaccg agagcacgaa
      661 gttgctgccg acgaagatgt cgacctcgcc ggtggtgagt tcgtcggtgg gcgtcagctc
      721 caccgtgtgc agcacggcga acagcgtgtc gccgtattcc tcgaccttgg gccgctggtg
      781 gccgtgcagc gcgtcctcca cggcgagttc gtgcaggccg aactccttct gcatcgtcag
      841 cagttcctcg tcggaggtgt ccttcatcgc gacccagacg aagcagcccg ggcgcgccag
      901 gtagtcgctg atctcggcga tgggaatgtc ggcgagcttg ctgccgttct cgtaggcgac
      961 gcagttgatc agcatgggca aagcaggtct ggagtcatca cactgtaacg aagacggcgt
     1021 caaggcgcgc cttgggcgtc gaaacgacga ttttgctgcg ctgcggtggc aacggcaggg
     1081 gcgctgggcc acaatgcccg ctccgaccaa cccgacggtt ccgcagagac gattcatggg
     1141 caagcagcaa cacggcgccg gcctcgcggc gatgacgctg ggcgccttgg gcgtcgtcta
     1201 cggggacatc ggcacgagcc cgctgtacac gctgcacgag atcttcgtcc ccgtctcggg
     1261 cgtgggcgtg ccgctcgacg cgcagcacat cgtcggcgcg gtgtcgacga tcttctgggg
     1321 cctgatgctg gtggtcacgc tcaagtacgt gatcctgatc ctgcgcgccg acaacaacgg
     1381 cgaaggcggc gcgatggcgc tgaccgcgct ggcggcgcat gcggccggcg gcacgccgca
     1441 gcggcgcacg ctcatcatgc tggtgggcat gttcggcgcc acgctgttct tcggcgacag
     1501 cgtcatcacg ccggccgtca cggtgatggg cgcggtcgag ggcctggagg tggtcacgcc
     1561 ggcgctccag gcctacatcg ttccgctgtc ggtgctcatc ctcgtcgcgc tgttctcggc
     1621 gcagcgcttc ggcaccggcg cgttcggcaa ggcgttcggg cccctgctcg tggtgtggtt
     1681 cggcctgctg gccgtcgtcg gcgcgctgca catctcccag gcgcccgaga tcctgtacgc
     1741 gctcaatccg ctgtacgccg tcgagttcct gctcgatcgc ggctggcacg tgctggccgc
     1801 gctcggcgcc atcgtgctgg cgctgaccgg tgctgaggcg ctgtatgccg acatggggca
     1861 cttcggcaag acgccgatcc gcctcgcctg gagcgcgctg gtgctgccgg cgctggcgct
     1921 caactacatg ggccagggcg cgctgctgat ccacgacgcc tcggccatcg agaacccgtt
     1981 cttccgcttg ttcccgtcga cctgggtgct gccgatggtg gcgttcgcgg ccgtggccgc
     2041 cgtcatcgcg tcgcaggcgg tgatctcggg cgcgttctcg atgaccaagc aggcgatcca
     2101 gctcggcttc ctgccgcgca tggaggtcgt ctacacctcg gtcaaggaag ccggccagat
     2161 ctacctgccg cgcgtcaact gggtgctgat ggtggcggtg gtgtgcgccg tcatcggctt
     2221 tggcagcgtc tccaagctga ccgcggccta cggcatcgcg gtgacggtga cgatgctgat
     2281 cgacaccgtg ctcacgtact tcgtcgtgcg ctacggctgg ggcttcccgc tgtggatcgc
     2341 gctgggcgcc accgggttct tcgcgatcat cgacggcctg ctggtggccg catgctcgct
     2401 caagttcctg cacggcggct ggttcccgct gctgctggcg ttggcgatgt tcgcgatcat
     2461 gtcgacgtgg aagcgcggcc gcgagctgct gctcgagagc atccggcgcg acgacccgga
     2521 cctgatcccg ttcatcacgg cgctggccga ggacgacatc acgcgcacgc cgcgcactgc
     2581 cgtcttcacc gtggccaacg tcggcaccgt gccgcaggcg ctgctgcaca acctcaagca
     2641 caaccaggtg ctgcacgaga ccaacgtcat cctcacggtc aagttccacg aggtcccgtg
     2701 gatcccgttc gccgaccgcg tgcaggtcga gccgctggcg cgcggcttct ggcgcgtgac
     2761 ggtcaactac ggcttcaaga acacgcccga catcccgcag gccctcacgc tgtgtcgcgc
     2821 gcatgggctg gacgtggacg agttcaccac cagctacttc cttagccgcg aggtggtggt
     2881 gcccacgcgc ggcgacggca tggcgcactg gcgcgaacgc ctgttcgccg cgatgacgcg
     2941 caatgccggc agcgtcgtgg agttcttccg gctgcccaac aacgcggtca tcgagctggg
     3001 cacgcgcgtg cagatatgac gccaggcgct gcgccggccg gttagcggcg acatcgactc
     3061 acgcgcccgg ccgctatggc gccgcggcgc ccgattgcct ccccacctgt cgaactcctg
     3121 tcgcccgcgc ggcgcggatc gcgcggcgat gccgtccggt ggccgcaggc aaccgctcgt
     3181 cggctcgccg cggcggccgc gccaacaagt tctcgaacgg ccgttcagcg cgctcaacgg
     3241 atcgtgccgg cgccgatatg gagggcagat gcccgatccg tttcgccgct cctggcgcag
     3301 ggacgtgcat cgtcttcaat gcacgtcacg gagaaccgca tgcgcctggc ccccggcacc
     3361 atccatttcc tcaccttggg cgtcgcctcg ctcgccatgc tgtccgcgtg cggcggcggc
     3421 ggctccgacg ccgcgccgac gccgccggcg cccaagccca ccgcgacgca cctgaccggc
     3481 tccgcggcgg tcggcgcccc gatgacgcaa ggcacgctgc gcgtgatcga cgccaccggc
     3541 gccgtcgtgg cgcacgacat ccccatcaac gccgacggca cctacgacgc cggcgtgctg
     3601 accggcaccg gcccgtggcg catcgaggcc tgcggctaca cgggcgccaa ctacggctgc
     3661 atctattccg tcgcgcaggc ggcgggcacg gccaacgtca cgccgctcac gagcgccatg
     3721 atcacgctgg ccaccggcca gaaggcggac gccgtgatgt ccgccgccag cgcgcccggc
     3781 gcgtcggcgc tcggtgccgc gcaggaccag ctgcgcagcg gcctggccgg cacgctcagc
     3841 gactccggcg tgccgccgga catcgacttc accaccggcg ccctgcaggc cggttcgcgc
     3901 tcgggctacg accgcgtgct cgacgccgtg gacgtgacga ccggcaccga caacggcgcc
     3961 ttcgtgcagg tcacgccgcg catgggcgac ggcaacctgt acat
//
LOCUS       DATCRO010000822         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_9980, whole genome shotgun sequence.
ACCESSION   DATCRO010000822 DATCRO010000000
VERSION     DATCRO010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9980"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..568)
                     /locus_tag="VIN75_22425"
     CDS             complement(44..568)
                     /locus_tag="VIN75_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8976988.1"
                     /translation="MAIDIPTLHTERLDLLPPTADCADLYRTFYTDADASQSYGGPLS
                     VVGSWTRLASDLGSWHLQGFGVWVMRDRERGELVGVCGFWQAPGWRRELTWWLLPRYR
                     SRGFAQEASEAVVEHAYEVFGWPSVETYMVDTNDQARALVRRLGGEQIDRFVAPDGAE
                     RDLFRIPRPARPAA"
     gene            complement(652..2004)
                     /gene="phoR"
                     /locus_tag="VIN75_22430"
     CDS             complement(652..2004)
                     /gene="phoR"
                     /locus_tag="VIN75_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY8976989.1"
                     /translation="MSWLLNRAMLAIFIALAGGGVGAVVGNLVGAPQLGPAVGAAVAV
                     AAWVMVDGVRALRLMNWLRGDLEREAPRDRGFWGEASYRIERALRQRDRAFTLEQERF
                     GQFMSAIEASPNGVLLLDAGDQIQWLNAVAATHFGLDPVRDRLQRVTNLVRSPAFVAY
                     LQAGVFDEPLVFSTPGSDSTLSVIVSRYGDGMRLVLTQDITERERTEAMRRDFVANVS
                     HEIRTPLTVLAGFVETISSLPLTDVERERVLSLMSQQTARMTTLVEDLLTLAKLEGSP
                     RPACDRWTPVARLFAQVESEARGLSAGRHTLRFDIQGQDELAGNDSEWTSAVANLVSN
                     AVRYTPAGGSITVSWSHRPDGNSEIEVRDTGPGIAREHLGRLTERFYRVDGSRSRDTG
                     GTGLGLSIVKHVVQRHGGELDIQSEPGRGSAFRIVVPAARVRPLAPAQPVAEEPSTVR
                     "
     gene            complement(2042..2743)
                     /gene="phoB"
                     /locus_tag="VIN75_22435"
     CDS             complement(2042..2743)
                     /gene="phoB"
                     /locus_tag="VIN75_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY8976990.1"
                     /translation="MSRILVVEDESAIAELVAINLRHAGFEVAIAETADAAQVEVDRV
                     LPELVVLDWMLPGQTGLALAKRWRAEARTRDLPIIMLTARSEEADKLSGLDAGADDYL
                     TKPFSTKELLARIRAVLRRKAPEALDSAVEVSGLRLDPATRRVTRGTQEVKIGPTEFR
                     LLHFFMTHTERVHSRTQLLDRVWGDHVFIEERTVDVHVKRLREALAPVQCSSLIETVR
                     GAGYRLTQQAAATSA"
BASE COUNT          418 a         1040 c         1079 g          412 t
ORIGIN      
        1 gccggcgccc gcggcatcgg ccgccgcgtc gcactgaggc gtttcaggcc gccggcctcg
       61 ccgggcgcgg gatgcggaac aggtcgcgct cggcgccgtc cggcgcgacg aagcgatcga
      121 tctgctcgcc gcccaggcgc ctgaccagcg cccgcgcctg gtcgttggtg tcgaccatgt
      181 aggtctcgac gctcggccac ccgaagacct cgtacgcgtg ctcgacgacg gcctcggagg
      241 cttcctgcgc gaagccgcgg ctgcggtagc gcggcagcag ccaccaggtg agctcgcggc
      301 gccagcccgg ggcctgccag aagccgcaca cgcccaccag ctcgccgcgc tcgcggtcgc
      361 gcatcaccca gacgccgaag ccctgcaggt gccaggagcc caggtcgctc gccaggcgcg
      421 tccacgatcc caccaccgac aaggggccgc cgtacgactg cgaggcgtcc gcgtccgtgt
      481 agaacgtgcg gtacaggtcg gcgcagtccg cggtgggcgg cagcaggtcg aggcgctcgg
      541 tgtgcagggt gggaatgtcg atggccatga gcggagtcta gggcctggcc ggtcgcccgg
      601 gtcgccgtgg aaacctccac gggattgcac ttagggatgc ggcggccccg ttcagcgcac
      661 cgtcgaaggt tcctcggcca ccggttgcgc cggcgcgagc ggacgcacgc gcgcggccgg
      721 cacgacgatg cggaaggccg agccgcggcc cggttcgctc tggatgtcga gctcgccccc
      781 gtggcgctgc accacgtgct tgacgatgga caggcccagg ccggtgccgc cggtgtcgcg
      841 cgagcggctg ccgtcgacgc ggtagaagcg ctcggtgagc cggcccaggt gctcgcgggc
      901 gatgcccggc ccggtatcgc gcacctcgat ctccgaattg ccgtcgggtc gatgcgacca
      961 cgagaccgtg atcgagccgc cggccggcgt gtagcgcacc gcgttgctga ccaggttggc
     1021 cacggcgctg gtccattcgc tgtcgttgcc ggcgagttcg tcctggccct ggatgtcgaa
     1081 gcgcagcgtg tggcggcccg cggaaagccc gcgcgcctcg ctctcgacct gcgcgaacag
     1141 gcgcgccacc ggcgtccagc ggtcgcacgc aggacgcggg ctgccctcga gcttggccag
     1201 cgtcagcagg tcctcgacga gcgtcgtcat gcgcgcggtc tgctgcgaca tcagcgacag
     1261 cacgcgctcg cgctccacgt cggtcagcgg cagcgaggag atcgtctcga cgaagccggc
     1321 cagcaccgtc agcggggtgc ggatctcgtg cgagacgttg gcgacgaagt cgcgccgcat
     1381 cgcctcggtg cgctcgcgtt cggtgatgtc ctgggtcagc accagccgca tgccgtcgcc
     1441 gtagcggctg acgatgacgc tcagcgtcga gtcgctgccc ggtgtagaga acaccagcgg
     1501 ctcgtcgaac acgccggcct gcaggtaggc gacgaacgcg ggcgagcgca cgaggttggt
     1561 gacgcgctgc aggcggtcgc gcacggggtc caggccgaag tgcgtcgccg cgacggcgtt
     1621 gagccattgg atctggtcgc cggcgtccag cagcaggacg ccgttgggcg aggcctcgat
     1681 ggcggacatg aactgcccga aacgctcctg ttccagcgtg aaggcgcgat ctcgctggcg
     1741 cagcgcgcgc tcgatgcggt acgacgcctc gccccagaag ccgcggtcgc gcggcgcctc
     1801 gcgctcgagg tcgccgcgca gccagttcat caggcgcagc gcgcgcacgc cgtcgaccat
     1861 cacccacgcg gcgacggcca ccgccgcgcc gaccgccggc ccgagctgcg gcgcgccgac
     1921 caggttgccg acgaccgcac cgacgccgcc gccggcgagc gcgatgaaga tggccagcat
     1981 ggcgcgattc agcagccagc tcacgtcgtt ccccggttcg gcaagacgaa ggctcgcggg
     2041 atcaggcgga agtggccgcg gcctgctgcg tcaggcgata gcccgcgccg cgcacggtct
     2101 cgatcagcga cgagcattgc accggggcca gcgcctcgcg caggcgcttg acgtggacgt
     2161 cgaccgtgcg ctcctcgatg aacacgtggt cgccccagac gcggtccagc agctgcgtgc
     2221 ggctgtgcac gcgctcggtg tgcgtcatga agaagtgcag caggcggaac tcggtcggac
     2281 cgatcttgac ttcctgcgtg ccgcgcgtga cgcgccgcgt ggccgggtcc aggcgcaggc
     2341 ccgacacctc gaccgccgag tccaaggcct cgggcgcctt gcgccgcagc acggcgcgga
     2401 tgcgcgccag cagctccttg gtggagaacg gcttggtgag gtagtcgtcg gcgccggcgt
     2461 ccaggcccga cagcttgtcg gcctcctccg agcgcgcggt cagcatgatg atgggcaggt
     2521 cgcgcgtgcg cgcctccgct cgccagcgct tggcgagcgc gaggcccgtc tggccgggca
     2581 gcatccagtc gaggacgacc agctcgggca gcacgcgatc gacctcgacc tgggcggcat
     2641 cggccgtctc ggcgatggcc acttcgaaac cggcgtggcg caggttgatc gcgaccagtt
     2701 cggcgatcgc ggattcgtct tcgacgacga ggatacggct catgctgttg ctctttcaga
     2761 agtcacgttg ttgttcgaag aaaccgcgcg gcgccgcgcg cggccgctcc ggagcaagcc
     2821 gcgccagccc ctcgggggcg gcgagcgcag cgagcggggg cgtcatacgc cgagttcctg
     2881 gcgttggcga cgcaggtgtt ccggcatctc cttgtagacg tcctcgaaca tcgaggcggc
     2941 gctcgggat
//
LOCUS       DATCRO010000823         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_943088, whole genome shotgun sequence.
ACCESSION   DATCRO010000823 DATCRO010000000
VERSION     DATCRO010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943088"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VIN75_22440"
     CDS             complement(<1..893)
                     /locus_tag="VIN75_22440"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8976991.1"
                     /translation="MRAEAAAARPLPSTRPALALHGHHVRRLEAVVDFVDAHLDDELS
                     LERLATVAAISPFHFHRLFHLWSGETLNEFVRRRRLDVAAGRLRHCPDEKITAVSLNC
                     GFASPEGFARAFRERFGMTPSTWRGRAQDDERWLRGMPPVTVTREPEMDVLFMRGRGD
                     FGLVAPALWDRFMSAVRALGLVGQPLLFMGLDDPGIVGPERCRMDACVQLPPGFGLAG
                     ACPPPLLHKRIPARWMATLRYEGPASDIADGWNALLGQWLPQAAYQLAIGAFRERYDP
                     GQCVPGSRQVRCELGMPVEPR"
     gene            complement(893..1921)
                     /locus_tag="VIN75_22445"
     CDS             complement(893..1921)
                     /locus_tag="VIN75_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8976992.1"
                     /translation="MPSKEKPLAEPATLLDVARLAGVSAATVSRILNGTARVTEDKRA
                     AVEKAIERLQFRPNFAAQSLRSGSTRTIGVLTQELESPYFTRGARGIEDGLQGSDYAP
                     IVVPGHWNPAEELDRARLLIARRVDAMVILGGNLQDEQVVDMARKLPIAITGRELHAP
                     NVFSLHCDQVEGACRAVRHLIDLGHTRIAHISGPVAYPDAVDRREGYMRAHSSAGLAV
                     DPRLLRVGTYMETGGSEAIKQLLDDGHPFTAVFCANDQTLWGARQVLYERGVRVPEDV
                     SLVGFDDLPQSAYMTPAVTTVHQPIYEMGRSAAHQLLAALGATPPRSDPVPDLRLVVR
                     GTTRPVAR"
     gene            2231..3484
                     /locus_tag="VIN75_22450"
     CDS             2231..3484
                     /locus_tag="VIN75_22450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8976993.1"
                     /translation="MRRRALLPLPLACLAARPALAAAGRATLTVAAFPDLDRSARIAI
                     ESWAASHPQVDVRLTTRAFDDHHTAMTTAIATGANLPDVMAVDMDYLGKFTQAGGLED
                     LDAPPYDARAVAPRLARFTLGPATGLAGHLRALPADIGPGALFWRADLLARAGLAEDD
                     LTRDWDSYIAAGVKLKQATGIYLLASAIDLKDILIRAGLADGQGVYFDDAGRPQVQSQ
                     RFVQAFALARAARRAGIDARIAAWSSEWSEGFRRDRVATQMMGSWFAGHLKNWIAPAQ
                     SGLWRSSPLPAGIAASWGGSFWAIPVRAGHKPLAWDFIRTLCLDRAQQLEAFRRLDAF
                     PALLAAQQDAYLAQPIAYLGGQAARLQWSAASERVPVLHVDRYDQVAKAIVEAELDQV
                     LQHDKPIPQALADARAAIERRVRRY"
BASE COUNT          494 a         1277 c         1255 g          472 t
ORIGIN      
        1 atgcgcggct cgacgggcat gcccagctcg cagcgcacct ggcggctgcc aggcacgcat
       61 tgccccgggt cgtagcgctc gcggaacgcg ccgatcgcga gctggtaggc ggcctgcggc
      121 agccattggc ccagcagcgc attccagccg tcggcgatgt cggacgcggg cccctcgtag
      181 cgcagcgtgg ccatccagcg cgccgggatg cgcttgtgca gcagcggcgg cgggcacgcc
      241 cccgcgaggc cgaagccggg cggcagctgc acgcaggcgt ccatgcggca gcgctcgggg
      301 ccgacgatgc cgggatcgtc caggcccatg aacagcagcg gctggccgac caggcccagc
      361 gcgcgcacgg cactcatgaa gcgatcccac agcgccggcg cgacgaggcc gaagtcgccg
      421 cggccgcgca tgaacagcac gtccatctcg ggctcgcgcg tgacggtgac cggcggcatg
      481 ccgcgcagcc agcgctcgtc gtcctgcgcg cggccgcgcc aggtcgaagg cgtcatgccg
      541 aagcgctcgc ggaacgcgcg cgcgaagccc tcgggcgaag cgaagccgca gttcagcgag
      601 acggccgtga tcttctcgtc ggggcagtgg cgcagccggc cggccgcgac gtccagccgc
      661 cgccggcgca cgaactcgtt gagcgtctcg ccgctccaca ggtggaacag gcgatggaag
      721 tggaacggcg agatcgcggc gaccgtggcc aggcgctcga gcgacagctc gtcgtccagg
      781 tgcgcgtcga cgaagtcgac caccgcctcg agccggcgca cgtggtggcc gtgcaacgcg
      841 agcgccggac gcgtggaggg cagtggccgg gcggcggcgg cttcggcgcg catcatctgg
      901 cgaccgggcg tgtcgtgccg cgcaccacca gtcgcaggtc gggaaccggg tcgctgcgcg
      961 gcggcgttgc gccgagcgcc gcgagcagct ggtgcgcggc cgaacggccc atctcgtaga
     1021 tcggctggtg cacggtggtc acggccgggg tcatgtaggc cgactgcggc aggtcgtcga
     1081 agccgaccag cgagacgtcc tccggcacgc gcacgccgcg ctcgtagagc acctggcgcg
     1141 cgccccacag cgtctggtca ttggcgcaga acacggccgt gaacgggtgg ccgtcgtcga
     1201 gcagctgctt gatcgcctcg ctgccgccgg tctccatgta ggtgcccacg cgcagcaggc
     1261 gcgggtcgac cgcgaggccg gcgctgctgt gcgcgcgcat gtagccctcg cggcggtcga
     1321 cggcgtcggg atacgcgacc gggccgctga tgtgcgcgat gcgcgtgtgg cccaggtcga
     1381 tgaggtggcg caccgcgcgg cacgcgccct cgacctggtc gcagtgcagc gagaacacgt
     1441 tgggcgcgtg cagctcgcgc ccggtgatgg cgatgggcag cttgcgcgcc atgtcgacga
     1501 cctgctcgtc ctgcaggttg ccgcccagga tcaccatcgc gtcgacgcgc cgcgcgatca
     1561 gcagccgcgc gcggtcgagt tcctccgcgg ggttccagtg acccggcacg acgatcgggg
     1621 cgtagtcgct gccctgcagc ccgtcctcga tgccgcgggc gccgcgcgtg aagtacggcg
     1681 actcgagttc ctgggtcagc acgccgatcg tgcgcgtgga gccgctgcgc aggctctggg
     1741 cggcgaagtt gggccggaac tgcaggcgct cgatcgcctt ctccaccgcg gcgcgcttgt
     1801 cctcggtgac gcgcgccgtg ccgttgagga tgcgcgagac ggtggccgcc gacacgcccg
     1861 ccaggcgggc gacgtcgagc agcgtggcgg gttcggcgag gggcttttcc ttggacggca
     1921 tgggcaggtg tcgcgaccgt gggcaccgcg tcggcgatcg cgcggcgtcg ccatcttagc
     1981 gacggcggca cgtgacgccg gagcgggttc ctgatgcgat gaagtctaat tcgtgaaatc
     2041 gttatcacgc cagacgggaa aacatgtagc ccgcaggacg tcggggtcgt gagaaccatc
     2101 gccataggca aatccctccg gaatgagggt attcaccaac tcggcctgaa accccattga
     2161 ccacctgaca acgttgtctg acactgaggt ctgaaaacga tatcaaatcc atccggagac
     2221 aagcttcccc atgcgccgcc gcgccctgct tcccctgccg ctcgcctgtc tggccgctcg
     2281 tcccgcgctc gccgcggcgg gccgcgccac gctgacggtg gccgcgttcc ccgacctcga
     2341 ccgcagcgcc aggatcgcca tcgagtcgtg ggccgcctcg catccgcagg tcgacgtgcg
     2401 gctgacgacc cgcgccttcg acgaccatca caccgcgatg acgacggcca tcgccaccgg
     2461 cgcgaacctg cccgacgtga tggccgtcga catggactac ctgggcaagt tcacgcaggc
     2521 cggcggcctg gaggatctgg acgcgccgcc gtacgacgcc cgcgccgtgg cgccgcgtct
     2581 cgcgcgcttc acgctcggtc cggccaccgg ccttgccggc cacctgcgcg ccctgccggc
     2641 cgacatcggc ccgggcgcgc tgttctggcg tgccgacctg ctcgcgcgcg ccggcctcgc
     2701 cgaggacgac ctgacacgcg actgggactc gtacatcgcc gccggcgtca agctcaagca
     2761 ggccacgggc atctacctgc tcgccagcgc gatcgacctg aaggacatcc tgatccgcgc
     2821 cggcctcgct gacggccaag gggtctactt cgacgacgcg ggacgcccgc aggtgcagtc
     2881 gcagcgcttc gtgcaggcct tcgcgctcgc gcgcgccgcg cgccgcgccg gcatcgacgc
     2941 gcgcatcgcc gcatggagca gcgaatggag cgagggcttc cggcgcgacc gcgtcgccac
     3001 gcagatgatg ggctcgtggt tcgcgggcca cctgaagaac tggatcgcgc cggcgcagtc
     3061 gggcctgtgg cgctcgtcgc cgctgcccgc cggcatcgcg gcgtcctggg gcggctcgtt
     3121 ctgggcgatt cccgtgcgcg ccgggcacaa gccgctcgcc tgggacttca tccgcacgct
     3181 gtgcctggac cgcgcgcagc agctggaggc gttccgccgg ctcgacgcct tcccggccct
     3241 gctcgccgcg cagcaggacg cctacctcgc gcagccgatc gcctacctcg gcggccaggc
     3301 cgcgcggctg caatggagcg ccgcgagcga gcgcgtgccg gtgctgcacg tcgaccgcta
     3361 cgaccaggtc gccaaggcca tcgtcgaggc cgaactcgac caggtgctgc agcacgacaa
     3421 gcccatcccg caggcgctcg ccgacgcccg cgccgccatc gagcgccgcg tgcgccgcta
     3481 ctgagacccg ccatgcac
//
LOCUS       DATCRO010000824         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_819342, whole genome shotgun sequence.
ACCESSION   DATCRO010000824 DATCRO010000000
VERSION     DATCRO010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819342"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VIN75_22455"
     CDS             <1..744
                     /locus_tag="VIN75_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8976994.1"
                     /translation="GSMLIKITYKGRMQAEVKAAEATETAEAESLRRQVLEAKMAMMQ
                     AQVEPHFLFNTLASIDHLIEFDPKRASQMQRNLISLLRASMPSMRSTDSGLRPLDLEL
                     AVVRPYLEILKVRMEERLTTEIDVPDGLLSAEFPPMMIQTLVENSIKHGLEPKAEGGH
                     LSVKAEIRHGKLAVTVCDTGLGFEAAAQGSTHGTGVGLANIRERLALLYGSKASLTIA
                     NNPVGGTIVTITVPYTTHTESDSDEGAKA"
     gene            741..1292
                     /locus_tag="VIN75_22460"
     CDS             741..1292
                     /locus_tag="VIN75_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976995.1"
                     /translation="MTDSIDPLDHAPEPDDRPARAAAAAPATSPAVAAPVPYRPRPSG
                     WARLFRWWLGLSLLAFLGVALCVFIGLGHGDFSPLHIVVADDGADGITINGLSTAGEA
                     LLAVGAGLLALLLLLLVPLLILIVVGAVAIAVICGVGVPLIALALALAVVTSPVWVVG
                     LLIWLAARRRDTNRLAASATMPA"
     gene            1339..2184
                     /locus_tag="VIN75_22465"
     CDS             1339..2184
                     /locus_tag="VIN75_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8976996.1"
                     /translation="MTTTPSSEAAAPVRGVIADDERLMRDQLKARLAEVWPELQIVGE
                     AKNGLEAVDLVAEHHPDVVFLDIRMPGLSGVDAARRIAQLPAGDDDEDSDASLPEIVF
                     ITAYDQYAIEAFEQGVADYVLKPAERERLGVTVSRIKQRLALRGTDDEPPAPHLQQLL
                     HKLSAKLNPSGTPPALKWIQATVGQAIQMIPIEDVLFFISDEKYTRVQTATLEALIRK
                     PIKELVDEVDPDLFWQIHRSTLVNTRCIAGVTRDLRGRQIVAIKGSNEKLEVSRSYTH
                     LFKGM"
     gene            complement(2201..2578)
                     /locus_tag="VIN75_22470"
     CDS             complement(2201..2578)
                     /locus_tag="VIN75_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8976997.1"
                     /translation="MSLLSPPQGGASLARSYRNLSEREIRARLDGETLSEIERVTAKA
                     ELLRRGAGDDAPDTAFISGFQPTSAMDNIGAVESESEPPVDTDDAAAAAAPRRRLWPV
                     LAVVALGIAGGLAWALHVHAIRF"
     gene            complement(2575..3273)
                     /locus_tag="VIN75_22475"
     CDS             complement(2575..3273)
                     /locus_tag="VIN75_22475"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEY8976998.1"
                     /translation="MSVDSSTTSVRRILPFIGAAAALLAIASAPWALGKPWLNFAFKP
                     LATLCVIAWAASGRADDGLVKRWIVIGLVFSLLGDIALLWPVQGFLAGLVAFLLGHLS
                     YLVALTRRVKFLASRPAFGAWAIVAASVLASLWAGVPADMRVPVLVYVSALSAMAAQA
                     TSVWLAHRGQPDAGKWRIVAIGAALFVLSDAILATDRFVGGVPLPTLWNLSLYWLGQW
                     FIARAALRASEVVS"
     gene            complement(3451..>3694)
                     /locus_tag="VIN75_22480"
     CDS             complement(3451..>3694)
                     /locus_tag="VIN75_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8976999.1"
                     /translation="IEEERRLMYVGITRARQTLAVSTLRRRKKGRETVAGIPSRFIAE
                     MKLHEAKSQEDPRERLRKLREALASKAASAPPAAAD"
BASE COUNT          580 a         1343 c         1271 g          500 t
ORIGIN      
        1 ggctcgatgc tgatcaagat cacctacaag ggccgcatgc aggccgaagt gaaggccgcc
       61 gaggccaccg agacggccga ggccgagtcg ctgcgccgcc aggtgctcga ggccaagatg
      121 gcgatgatgc aggcgcaggt cgagccgcac ttcctgttca acacgctggc ctccatcgac
      181 cacctgatcg agttcgaccc gaagcgggcc agccagatgc agcgcaacct gatctcgctg
      241 ctgcgcgcca gcatgccgtc gatgcgctcc accgactccg gcctgcgccc gctggacctg
      301 gaactggccg tcgtgcggcc ctacctcgag atcctcaagg tgcgcatgga agagcgcctg
      361 accaccgaga tcgacgtgcc cgacggcctg ctgtcggccg agttcccgcc gatgatgatc
      421 cagacgctgg tcgagaactc gatcaagcac ggcctggagc ccaaggccga gggcggccac
      481 ctgtcggtca aggccgagat ccgccacggc aagctggccg tgaccgtctg cgacacgggc
      541 ctcggattcg aggccgccgc gcagggctcg acgcacggca caggcgtcgg cctcgccaac
      601 atccgcgagc gcctcgcgtt gctgtacggc agcaaggcct ccctgacgat cgccaacaac
      661 ccggtgggcg gcacgatcgt gacgatcacc gtgccctaca ccacccacac cgaatccgac
      721 agcgacgaag gagccaaggc atgaccgatt ccatcgaccc tctcgaccac gcgcccgaac
      781 ccgacgaccg gcccgcccgc gccgccgcgg ccgcacccgc cacttcgccg gccgtggcgg
      841 cgcccgtgcc gtatcgcccg cgccccagtg gctgggcgcg cctgttccgc tggtggctgg
      901 gcctgagcct gctcgccttc ctgggcgtgg cgctgtgcgt gttcatcggc ctgggccacg
      961 gcgacttctc gccgctgcac atcgtcgtcg ccgacgacgg cgccgacggc atcacgatca
     1021 acggcctgag caccgccggc gaggcgctgc tggcggtcgg cgcgggcctg ctcgccctgc
     1081 tgctcctgct gctggtgccg ctcctgatcc tgatcgtggt gggcgccgtc gccatcgcgg
     1141 tgatctgcgg cgtcggcgtg ccgctcatcg cgctcgccct ggccctcgcg gtggtgacgt
     1201 cacccgtgtg ggtcgtgggc ctgctcatct ggctggccgc gcgccggcgc gacacgaatc
     1261 gcctcgccgc ttcggctacg atgcccgcct gatgacggct cgcccaccgg cgggctcgcg
     1321 cagcaccaag gctgacccat gaccacgact ccttcttccg aagccgccgc cccggtgcgc
     1381 ggcgtgatcg ccgacgacga gcggctgatg cgcgaccagc tcaaggcgcg cctggccgag
     1441 gtgtggcccg agctgcagat cgtcggcgag gccaagaacg gcctcgaagc cgtcgacctg
     1501 gtggccgagc accatcccga cgtcgtgttc ctcgacatcc gcatgccggg cctgtcgggg
     1561 gtcgacgcgg cgcgccgcat cgcgcaattg cccgccggcg acgacgacga ggacagcgac
     1621 gcgtcgctgc ccgagatcgt gttcatcacc gcctacgacc agtacgcgat cgaggcgttc
     1681 gagcagggcg tggccgacta cgtgctcaag cccgccgagc gcgagcgcct gggcgtcacg
     1741 gtctcgcgca tcaagcagcg cctcgcgctg cgcggcaccg acgacgagcc gccagcgccg
     1801 cacctgcagc agctccttca caagctctcg gccaagctca acccgagcgg cacgccgccc
     1861 gcactgaagt ggatccaggc caccgtcggc caggccatcc agatgatccc gatcgaggac
     1921 gtgctgttct tcatctccga cgagaagtac acgcgcgtgc agaccgccac cctggaggcg
     1981 ctgatccgca agccgatcaa ggagctcgtc gacgaggtcg atcccgacct gttctggcag
     2041 atccaccgct cgacgctggt caacacgcgt tgcatcgcgg gcgtgacgcg cgacctgcgc
     2101 ggacgccaga tcgtcgcgat caagggcagc aacgagaagc tggaagtcag ccgctcctac
     2161 acgcacctgt tcaagggcat gtgaggcgcg ggcggggcgg ctagaagcga atggcgtgaa
     2221 cgtgcagcgc ccaggcgagg ccgccggcga tgcccagcgc caccaccgcc agcacgggcc
     2281 agaggcgtcg gcgcggcgct gctgcggcag cggcgtcgtc cgtgtcgacc ggcggttcgc
     2341 tctcgctctc gacggcgccg atgttgtcca tcgcgctggt cggctggaag ccgctgatga
     2401 aggcggtgtc gggtgcatcg tcgccggcgc cgcggcgcag cagttcggcc ttggccgtga
     2461 cgcgttcgat ctcgctgagc gtctcgccgt ccagccgcgc gcggatctcg cgctcggaca
     2521 ggttgcggta gctgcgggcc agcgaggcgc cgccttgcgg tggggaaaga agactcaaga
     2581 gacgacctcc gatgcgcgga gggccgcacg agcgatgaac cactggccga gccagtagag
     2641 ggacaggttc cacagcgttg gcagcggcac gccgccgacg aacctgtcgg tcgcgaggat
     2701 cgcgtccgac agcacgaaca gggccgcgcc gatggcgacg atgcgccact tgccggcgtc
     2761 gggctggccg cggtgcgcaa gccacaccga cgtggcctgc gcggccatcg ccgacagcgc
     2821 ggagacgtag accagcaccg gcacgcgcat gtcggccggc acgccggccc acagcgacgc
     2881 gagcacgctg gccgcgacga tcgcccacgc gccgaaggcc ggtcggctgg cgaggaactt
     2941 cacgcgccgc gtcaacgcga cgaggtacga caggtggccc agcaggaatg cgaccaggcc
     3001 cgcgagaaag ccctgcacgg gccacagcag cgcgatgtcg cccagcagcg agaacacgag
     3061 gccgatgacg atccagcgct tgacgaggcc gtcgtcggcg cggccgctcg cggcccacgc
     3121 gatcacgcac agggtggcca gcggcttgaa cgcgaagttg agccagggct tgccgagcgc
     3181 ccagggcgcg gacgcgatgg ccagcagcgc ggccgcggcg ccgatgaacg gcaggatgcg
     3241 acgcacggac gtggtcgagg aatcgacgga catgggacgc gagtgtcccc gtcgcgttgc
     3301 gcaacccact cagggtaagc ccgttccagt ccccggtttt ggggaaagcc gcacgcggcc
     3361 gcgtgaactc gcgcacgccg ggccgagcct gtctagacag gcctctccag gtccgcgtgg
     3421 gccgcgcgcg acacgcgatg gcgccgcgcg tcagtccgcc gcggcgggcg gcgcgctcgc
     3481 ggccttgctg gccagcgcct cgcgcagctt gcgcaaacgt tcgcgcggat cttcctgcga
     3541 cttggcctcg tgcagcttca tctccgcgat gaagcggctg gggatgccgg ccaccgtctc
     3601 gcggcctttc ttgcgccggc gcagcgtgct gaccgcgagc gtctggcgcg cccgcgtgat
     3661 gcccacgtac atcaggcggc gctcctcctc gatg
//
LOCUS       DATCRO010000825         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_875279, whole genome shotgun sequence.
ACCESSION   DATCRO010000825 DATCRO010000000
VERSION     DATCRO010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875279"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VIN75_22485"
     CDS             complement(<1..1178)
                     /locus_tag="VIN75_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977000.1"
                     /translation="MTAFKPARALQDLLLVALMLCALAGVIALAYHVAFRWGVNEARR
                     TEQRHLQFLSSDLALALDKYETLPAALAGYPDQLDLLRHPEDTARRDAVNHRLEELAS
                     RIHVTQIYLMDNRGTTLAASNWNMAHTFVGQNFAFRPYFRDALAQGSGRYYAVGTTSL
                     EPGYFLAHRVPDVLAPGQAPQGVIAVKIQLEDIESSWGHDQSLLMLADQHGVVFLSNR
                     PEWKFHTLAKLDAATLAQIRAERQYGTAGLESVPMRDDGDGLAKVAVAEPAGRGRMRW
                     LQVVGERRDLAHMKWTLLSFSTTGEIANLARSYAAMAGFAFAFASLSALYARLRHRRN
                     VERRRAQRTLERVHAELEQRIGERTAVLVNANDELESKIRELDRTQAALRAMQDELVQ
                     A"
     gene            complement(1186..2505)
                     /locus_tag="VIN75_22490"
     CDS             complement(1186..2505)
                     /locus_tag="VIN75_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977001.1"
                     /translation="MAAAPAAPSIEDTIELKRVKAIFIGSIGNLVEWYDFYCFAAFSL
                     YFSAAFFPAQDPTTQMMSTAGIFALGFFVRPIGGVLFGRMGDRLGRRAALMASVTLMC
                     LGSLIIACAPTYATAGAAAPAVLLLARLLQGLSLGGEYGSSATYLSEMATSRHRGFYS
                     SFQYVTLIGGQLVALLVLLALQNVFLDPAELKAWGWRIPFLIGAGLALVALAMRRDLA
                     ETQSFNKAREGGATRGGLRQLMQHPREVMIVVGLTMGGTLAFYVYTTYMQKFLKLSVG
                     LTDAQTTAVSAGSLVLAMLLQPLYGALSDRIGRRPLLLAFAVLGAVFTVPLLTAIRSA
                     HGPWQAFGLIACAWLIVSGYTAINAVVKAELFPASIRATGVGVPYAIAVSVFGGSAEY
                     VALWFKQAGNESGFFWYVSAVICCSLLVYAGMRETSRHSRIDAESSD"
     gene            2781..>3057
                     /locus_tag="VIN75_22495"
     CDS             2781..>3057
                     /locus_tag="VIN75_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977002.1"
                     /translation="MKTVTRRALVRVMQGRRHAARDEPAPAHDYRRQLMTRSTRSRFW
                     MALATALLATACDGDGPRELRVGLLVAQTGASGARGKDLVQGAMLAAE"
BASE COUNT          477 a         1056 c         1105 g          419 t
ORIGIN      
        1 gcctgcacga gctcgtcctg catcgcccgc agcgcggcct gcgtgcgatc gagttcgcgg
       61 atcttggact cgagttcgtc gttggcgttg accagcaccg ccgtgcgctc gccgatgcgc
      121 tgctcgagct cggcgtgcac gcgttcgagt gttcgctgcg cgcgccggcg ctcgacgttg
      181 cgccggtggc gcagtcgcgc gtacagcgcc gagagcgacg cgaacgcgaa cgcgaagccc
      241 gccatggccg cgtagctgcg cgcgaggttg gcgatctcgc cggtggtcga gaacgacagc
      301 agcgtccact tcatgtgggc gaggtcgcgc cgctccccca ccacctgcag ccagcgcatg
      361 cggccgcgtc ccgcgggctc ggcgacggcg accttggcca ggccgtcgcc gtcgtcgcgc
      421 atcggcaccg actcgaggcc ggcggtgccg tactgccgct cggcccggat ctgcgccagc
      481 gtggccgcgt ccagcttcgc cagcgtgtgg aacttccact cgggacgatt cgacaggaag
      541 acgacgccgt gctggtcggc gagcatcagc agcgactggt cgtggcccca gctcgactcg
      601 atgtcctcga gctggatctt gaccgcgatg acgccctgcg gcgcctgccc gggggccagc
      661 acgtcgggca cccggtgcgc caggaagtag cccggctcca gcgacgtggt gccgacggcg
      721 tagtagcggc cggacccctg cgcgagggcg tcgcggaagt agggccggaa cgcgaagttc
      781 tggccgacga acgtgtgcgc catgttccag ttgctcgcgg ccagggtggt gccgcggttg
      841 tccatcaggt agatctgcgt gacgtggatg cggctcgcca gctcctcgag ccggtggttg
      901 accgcgtcgc ggcgcgccgt gtcctccggg tggcgcagta ggtcgagctg gtcggggtac
      961 cccgccagcg cggcgggcag ggtctcgtac ttgtcgagcg ccagcgccag gtcggacgac
     1021 aggaactgca ggtggcgctg ctccgtgcgg cgcgcctcgt tcacgcccca gcggaacgcc
     1081 acgtgatacg cgagcgcgat caccccggcc agggcgcaca acatcagcgc caccaggagc
     1141 agatcctgga gcgcccgcgc cggcttgaat gccgtcatgg acgattcagt cgctggattc
     1201 ggcatcgatc ctcgagtgac gactggtctc gcgcatgccg gcgtacacca gcagcgagca
     1261 gcagatgacc gcggaaacgt accagaagaa tccgctctcg ttgccggcct gcttgaacca
     1321 cagcgcgaca tattccgcgc tgccgccgaa cacggacacc gcgatggcgt agggcacgcc
     1381 cacgccggtc gcgcggatcg acgccgggaa cagctcggcc ttcacgactg cgttgatggc
     1441 agtgtagccc gacacgatca gccaggcgca ggcgatcagc ccgaaggcct gccacggccc
     1501 gtgcgcactg cggatggccg tcagcagcgg cacggtgaag accgctccga gcaccgcgaa
     1561 cgcgagaagc agcggccggc ggccgatgcg gtccgacagc gcgccgtaca gcggctgcag
     1621 cagcatcgcc agcaccagcg agccggccga gacggccgtc gtctgcgcgt ccgtcaggcc
     1681 cacggacagc ttgaggaact tctgcatgta ggtcgtgtag acgtagaacg ccagagtgcc
     1741 gcccatcgtc aggccgacca cgatcatcac ctcgcgcggg tgctgcatca gctggcgcag
     1801 gccgccgcgc gtcgcaccgc cttcgcgcgc cttgttgaac gactgcgtct ccgcgaggtc
     1861 gcgccgcatc gccagcgcca cgagcgcgag gcccgcgccg atgaggaagg ggatgcgcca
     1921 gccccaggcc ttgagctcgg ccgggtcgag gaagacgttc tgcagcgcca gcagcacgag
     1981 cagcgccacc agctgcccgc cgatgagcgt cacgtactgg aagctcgagt agaagccgcg
     2041 atgccgggag gtggccatct cggacaggta cgtcgcgctg gagccgtact cgccgcccag
     2101 gctcaggccc tgcagcagcc gcgccagcag cagcacggcg ggcgccgcgg cccccgcggt
     2161 ggcgtaggtc ggggcgcagg cgatgatcag cgagcccagg cacatcagcg tgaccgaggc
     2221 catcagcgcg gcgcgccggc cgagccggtc gcccatgcgg ccgaacagca cgccgccgat
     2281 gggccgcacg aagaagccga gcgcgaagat gccggcggtc gacatcatct gcgtggtcgg
     2341 atcctgcgcg ggaaagaagg ccgccgagaa gtacagcgag aacgcggcga agcagtagaa
     2401 gtcgtaccac tcgacgagat tgccgatcga gccgatgaag atcgccttga cgcgcttgag
     2461 ctcgatggtg tcttcgatgg agggcgcggc gggcgcggcg gccaggtcgg cctgcgtggg
     2521 gctgttcaat gtctgcacgg gtcgtctcct cgtggatgtt ggatgggcgg gtcgaccagg
     2581 cgtcgacgcg acgcaccgca tctgcaggtt gtgtgccatg cggccgcggc cggggcgaga
     2641 cgcgatgcgc gccgccggat gcccgtgagg gcgcaccggc gcgccgtggc ggacgcggcg
     2701 gcattccacc gtggcccgtg tcggcgcggc gagattccgc cgccgcgggg ccagccgcgc
     2761 tgtcgcctgc aggtccgacc gtgaagaccg tcacgcgccg cgcgttggta cgcgtcatgc
     2821 aaggccgacg tcacgcggcg cgggatgaac ccgcgcccgc ccacgactac aggagacagc
     2881 tcatgacgag atcgacgcgc tcacgtttct ggatggcgct cgctaccgcc ctgcttgcca
     2941 cggcatgcga cggcgacggc ccgcgcgaat tgcgcgtggg cctgctggtc gcgcagaccg
     3001 gcgcatccgg cgcccgcggc aaggacctgg tgcagggcgc gatgctcgcc gccgagg
//
LOCUS       DATCRO010000826         9311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_380598, whole genome shotgun sequence.
ACCESSION   DATCRO010000826 DATCRO010000000
VERSION     DATCRO010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380598"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIN75_22500"
     CDS             complement(<1..283)
                     /locus_tag="VIN75_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8977003.1"
                     /translation="MTPILHSLLETDLYKFTMWQTMLHRHPQAQAEYRFVCRNAPAFP
                     LSELLDEVNEQLDHLCALSFTEDELAYIGALRYIKNDFVDFLRIFRFQRR"
     gene            complement(316..1170)
                     /locus_tag="VIN75_22505"
     CDS             complement(316..1170)
                     /locus_tag="VIN75_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY8977004.1"
                     /translation="MKTATQLLIIDPQNDFCDVPDAYRPVDPLTGAPVAPALPVAGAH
                     ADMLRTAQFISAAGSWLDEITVTLDSHHQVDIAHPTFWQDANGQAVAPFTQITAAQVR
                     LGTYLPRDPSALPRTLAYLDDLEARGRYVLMVWPVHCEMGTWGHDVHADVRDAYNRWE
                     RARLRNVRKVGKGENPWTEHYSAIQAEVPDPQDEQTQLNTALLAALGRADLLFIAGEA
                     SSHCVRATTEHIVANLPGGRPERLVLLTDCMSPVAGFEAQHQAFLDDMRAQGVRLATS
                     TEALGLLA"
     gene            1340..1603
                     /locus_tag="VIN75_22510"
     CDS             1340..1603
                     /locus_tag="VIN75_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEY8977005.1"
                     /translation="MAVHPSHPEIIKRLKRAEGHLRSIVTMLEEGRDCLEIAQQMQAV
                     ESAVGNAKRTLVHDHIDHCLEHAVDGVGARDAITQFKAITKYL"
     gene            1600..2568
                     /locus_tag="VIN75_22515"
     CDS             1600..2568
                     /locus_tag="VIN75_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HEY8977006.1"
                     /translation="MTDFHTLLAGGNAWLFLPSAILLGALHGLEPGHSKTMMAAFIVA
                     VRGTVMQAVLLGLAATVSHTAIVWLIALGGMYFGQRWSAEASEPWMQLISAVLIIGVA
                     AWMLWRTAQDKAACFHDHGDGAHDHDHDHGGHAHAPAPALARATRDAQAHSHGDHGHA
                     REDHAGHDHDHDHGASCGHDHGHAHAKAAPLVDAGDGDAHARAHAADIQRRFAGRDVT
                     TGQIVMFGLTGGLIPCPASITVLLLCLQLKRIALGATLVLGFSVGLALTMVASGVVAA
                     VGLKHLSKRMGSGRFSELAAKAPYFSGGLILLVGLYVGYQALHALV"
     gene            2661..3557
                     /locus_tag="VIN75_22520"
     CDS             2661..3557
                     /locus_tag="VIN75_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8977007.1"
                     /translation="MPPVPPSIACALTLCLTLCVTPAAAAPAPPRTEHFPSADGRTEL
                     VGYLFEPTSPGPHPAIVLLHGRGGPYSANVNRDCTLVAPGHESPCDPSTLSRRHRMWG
                     EYWAAHGVVALLVDSFGPRGRAHGYGRFTHDDPEREAVNERTVRPLDAEGALAWLAAR
                     RDVRPDRVALQGWSNGASTALNAMMRQADGLAADKSRRFRAALVFYPGCGPKALLSRH
                     VAVDAPVWVFLGSTDEEVSPAVCAQVLSRASGGPVSVTTYPGATHDFDDPGEARRSDA
                     ANRAARADAMGRAAALLDGVAP"
     gene            3612..3941
                     /locus_tag="VIN75_22525"
     CDS             3612..3941
                     /locus_tag="VIN75_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEY8977008.1"
                     /translation="MAATHRKPGTPPARKWSQHVTETSNAMDLQPDVFKLEDPKQIAR
                     SLKRSAEHSDRRKSDPYRSAMSMLTFYINRAGHNLAASQRKRLEDVKDELRVLFHKPP
                     PRRRTTH"
     gene            4024..5403
                     /locus_tag="VIN75_22530"
     CDS             4024..5403
                     /locus_tag="VIN75_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY8977009.1"
                     /translation="MSSLFRRQVQPDDAQVEAARAAAIPLDGGDGEFDELLRRIGNAR
                     FVLLGEASHGTHEFYEARARITRRLIAERGFDAVAIEGDWPDAYRVNRHVRGLGDDRD
                     ASGALAGFQRFPTWMWRNTAVVDFVDRLRAWNDGRPAAARAGFYGLDLYSLNASMSSV
                     LEYLDREDPAVAARARERYACFEPFGGDSQVYGLMTGLGSTPSCEEGVIRTLVDLQHR
                     RARGLPVAGDDPEAQFDAEQNAALVRDAERYYRGMYLSDVSSWNERDTHMARTLWEID
                     RHLSRPGRPARIVVWAHNSHLGDASHTEMGQARGELNLGQLVRERFPQDCWLVGFTTH
                     DGAVTAATEWDAKAEHKRVNPSRGDSIEGVLHATGLHRFLLPFRDDPRLSRQFEHPLL
                     ERAIGVIYRPDTERQSHYFHAQVARQFDALIHVDRTRAVEPLEADEPWAAGRAAHAGE
                     APETFPQGL"
     gene            5454..5831
                     /locus_tag="VIN75_22535"
     CDS             5454..5831
                     /locus_tag="VIN75_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY8977010.1"
                     /translation="MQSPGHREHPEHHVIESPVQGRLRAQLGADVLAESDDVIEVDED
                     GNPPRYYFPRDSVRMDRMSASDTHTYCPFKGEASYFTMEFLDGQMPDAAWTLKDPYDE
                     HADLKDRVAFYSEKFDGLQFKRS"
     gene            complement(5838..7052)
                     /locus_tag="VIN75_22540"
     CDS             complement(5838..7052)
                     /locus_tag="VIN75_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY8977011.1"
                     /translation="MNTISATYPLVRRPVPLWLLVLVTFSGTLAMHMFVPALTIAAQS
                     LHVDAATIRMTISVYIFGLAVGQLVYGPLSDVFGRRPTLLAGLALYTAGGLAATLAPN
                     VGVLVAARLVQAAGGCAGLLLGRAIVRDTAESNDAVRRLALMSLMTMVGPGLAPLAGG
                     LLAGAFGWRAIFVLLTALGVLNFTVSWRLLPETGKPSGHISAGGVARDYRALLASPRF
                     VGFAFGGGCATTSFYGFIAAAPFIFINELHRPIGEVGVYLGLLVVGVSTGNILASRLV
                     GRFTIERLLMAGNLASLAAAGAVLAALLAWHPGAFAIEALMIAYCVGAGLCSPAVSTL
                     SISVDPRLIGSAAGLYGFTQMTIGALCTTLAGLGHDPAVAAAAVLVGAALLAQASFRV
                     ALRTPARTSAAA"
     gene            complement(7189..8133)
                     /locus_tag="VIN75_22545"
     CDS             complement(7189..8133)
                     /locus_tag="VIN75_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977012.1"
                     /translation="MKSPRPILPRTVTAALLALAGASAFAQDAADAPDVDEALVADAD
                     LAALPSDALDHAVWATLLPSNDAADPTIVRSAISAVLAAPDTKPRSIVPITARKDKPA
                     IVTVRIDPNAVMAPPTKLMFDSNQVVQIHADGMPAPNGPDRDPLVRVLPRQADPNAVT
                     FGARWSAQDRIQAPLLSGLSWDAHADLVSGAAPARAGNVRRSLRITAMWDSPEDLSIG
                     FTPGFTRGGGREFEHYVTGLEVSTVDKTKLARWRSYVELSGEKLAFNNVIDNATAHVA
                     AGASYATSSSTQLDFNVSRGTTWTSNDLTSNVGLSVKF"
     gene            8501..>9311
                     /locus_tag="VIN75_22550"
     CDS             8501..>9311
                     /locus_tag="VIN75_22550"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEY8977013.1"
                     /translation="MSHRTTAIAIARAVLTTPVSRFGILERLPQCVRFPQQAHRDLVD
                     ALFAAGLASHWTIDQVADVLEKDDTFWAAIAVSRQRPLVHGWFMTRAGTLAPAPTPLR
                     GVARPAWPGVGSLADDLGVPVPGLWRLTRSASWQRQAPLGQQHYRYELLPKRTGGWRL
                     LEVPEPYLRHLQRQVLDRLLAHVPPHEAACAYVRGRSVTDHARAHVGQAVLLKFDLQD
                     FFATVRASRVHAAFATLGYPAPVARVLARLCTTATPEPVLERLRTEGGFAWT"
BASE COUNT         1375 a         3334 c         3336 g         1266 t
ORIGIN      
        1 agcggcgctg gaagcggaag atgcgcagga agtcgacgaa gtcgttcttg atgtagcgca
       61 gcgcgccgat gtaggcgagc tcgtcctcgg tgaacgacag cgcgcacagg tggtcgagct
      121 gctcgttcac ctcgtccagc agctccgaca gcgggaacgc cggcgcgttg cggcagacga
      181 agcggtactc ggcctgcgcc tgcggatgtc gatgcagcat ggtttgccac atcgtgaact
      241 tgtagaggtc ggtctcgagc aggctgtgga ggatcggggt catggcgcca ttctcatcgt
      301 tgacgggtcg accgctcagg ccagcaggcc cagcgcctcg gtcgacgtcg ccaggcgcac
      361 gccctgggcg cgcatgtcgt cgaggaacgc ctggtgctgc gcctcgaagc cggccacggg
      421 gctcatgcag tcggtcagca gcacgaggcg ctcggggcgg ccgccgggca ggttggcgac
      481 gatgtgctcg gtggtggcgc gcacgcagtg gctgctggcc tcgccggcga tgaacagcag
      541 gtcggcgcgg ccgagggcgg ccagcagcgc ggtgttgagc tgcgtctgtt cgtcctgtgg
      601 gtcgggcacc tcggcctgga tggcgctgta gtgctccgtc cacgggttct cgcccttgcc
      661 gaccttgcgc acgttgcgca gccgggcgcg ctcccagcgg ttgtaggcgt cgcgcacgtc
      721 ggcgtggacg tcgtggcccc aggtgcccat ctcgcagtgc acgggccaga ccatcagcac
      781 gtagcggccg cgcgcctcga ggtcgtcgag gtaggccagc gtgcgcggca gggcggacgg
      841 gtcgcgcggc aggtacgtgc ccaggcgcac ctgggcggcg gtgatctgcg tgaacggggc
      901 gacggcctgg ccgttcgcgt cctgccagaa ggtggggtgg gcgatgtcga cctggtggtg
      961 ggagtcgagc gtgacggtga tctcgtcgag ccacgaaccg gcggcgctga tgaactgtgc
     1021 cgtgcgcagc atgtcggcgt gggcgccggc cacgggcagg gcgggcgcga cgggcgcgcc
     1081 ggtgagcggg tcgaccggtc gatacgcatc cggcacatcg cagaagtcgt tctgcgggtc
     1141 gatgatcagg agctgggtgg cggtcttcat ggggtgcttc cttgttgtcc gtgagttcac
     1201 tatacaccaa caagttgtgc aatacaacta ataaaccgga tcagcctccc tcccccggaa
     1261 ggaaacccct cgggccatcg atgttcagac cgcggaaggc tggatcggtt atcctatcct
     1321 cccccacagg atacgagaca tggccgtcca cccctcccat cccgagatca tcaagcgcct
     1381 gaagcgcgcc gagggccacc tgcgcagcat cgtcacgatg ctggaggagg gccgcgactg
     1441 tctcgagatc gcccagcaga tgcaggcggt cgagagcgcc gtgggcaacg ccaagaggac
     1501 gctggtccac gaccacatcg accactgcct ggagcacgcc gtcgacggcg tgggcgcccg
     1561 cgacgcgatc acccagttca aggcgatcac caagtaccta tgaccgattt ccacaccttg
     1621 ctggccggcg gcaacgcctg gctgttcctg ccgagcgcca tcctgctggg cgcgctgcac
     1681 ggcctggagc ccggccactc caagaccatg atggcggcgt tcatcgtcgc cgtgcgcggc
     1741 accgtcatgc aggcggtgct gctgggcctg gcggccaccg tctcgcacac ggccatcgtg
     1801 tggctgatcg cgctgggcgg catgtacttc ggccagcgct ggagcgccga ggccagcgag
     1861 ccctggatgc agctgatctc ggcggtgctg atcatcggcg tcgcggcgtg gatgctgtgg
     1921 cgcacggcgc aggacaaggc ggcgtgcttc catgaccatg gggacggggc gcatgaccat
     1981 gaccacgacc atggtggcca cgcgcacgcc cccgcgcccg ccctcgcgcg cgcgacgcgt
     2041 gacgcgcagg cgcactcgca cggcgatcat ggccatgcgc gcgaggatca tgcgggtcac
     2101 gaccacgacc acgaccacgg cgcgtcgtgc ggccacgacc acggccatgc gcacgccaag
     2161 gccgcgccgc tcgtcgacgc gggcgacggt gacgcccacg cccgcgcgca cgcggcggac
     2221 atccagcgcc gtttcgccgg ccgcgacgtc accaccggcc agatcgtgat gttcggcctg
     2281 accggcggcc tgatcccgtg ccctgcctcg atcaccgtgc tgctgctgtg cctgcagctc
     2341 aagcgcatcg cgctcggcgc gacgctggtg ctcggcttct ccgtgggcct cgcgctgacg
     2401 atggtggcct cgggcgtggt cgccgcagtg ggactgaagc acctgagcaa gcgcatgggc
     2461 agcgggcgtt tcagcgagct ggcggccaag gcgccgtact tctcgggcgg gttgatcctg
     2521 ctggtgggcc tgtacgtcgg gtaccaggcg ctgcacgcgc tggtctgagg agttcctccg
     2581 atccccctgc gagagcccag gacgaggcgt gcgcgccacc gcgccttccc gctttctcgc
     2641 gccggcgcga cactgcaggc atgccacccg ttccaccgtc gatcgcctgc gctctcacac
     2701 tctgcctgac gctttgcgtc acgccggcgg cggccgcgcc tgcgccgccg cgcaccgagc
     2761 acttccccag cgccgacggc cgcaccgaac tcgtcggcta cctgttcgag ccgacgagtc
     2821 ccggcccgca tccggccatc gtcctgctgc acggccgcgg cggcccctac tcggcgaacg
     2881 tgaaccgcga ctgcacgctg gtcgcgcccg gccatgaatc gccgtgcgac ccgtcgaccc
     2941 tgtccaggcg ccaccggatg tggggcgagt actgggccgc gcacggcgtc gtggcgctgc
     3001 tggtcgacag cttcggcccg cgcggccgcg cgcacggcta cgggcgcttc acgcacgatg
     3061 acccggagcg cgaggccgtc aatgagcgca cggtgcgccc gctcgacgcc gagggcgcgc
     3121 tcgcatggct cgccgcgcgg cgcgacgtgc ggcccgaccg cgtggcgctg cagggctggt
     3181 cgaacggcgc gagcacggcg ctgaacgcga tgatgcggca ggccgatggc cttgccgccg
     3241 acaagtcccg gcgcttccgc gcggcgctgg tgttctatcc tggctgtggc cccaaggccc
     3301 tgctgtcgcg ccacgtcgcg gtcgacgcgc ccgtgtgggt gttcctcggg tcgaccgacg
     3361 aggaggtctc gcccgccgtc tgcgcgcaag tgctttcgcg cgcgagcggt ggccccgtct
     3421 cggtgacgac ctacccgggc gcgacgcacg acttcgacga ccccggcgag gcgcgccggt
     3481 ccgacgccgc caaccgcgcc gcccgggccg acgccatggg acgcgcggcg gcgctgctgg
     3541 acggcgtggc gccctgagcc gcggcgggtg tcccgcttgc tcggacccgc catcgccaca
     3601 cgggagccga catggccgcc acgcaccgca agccgggcac gccgcccgcg cgcaaatggt
     3661 cgcagcacgt caccgagacg agcaacgcga tggacctgca gcccgacgtc ttcaagctcg
     3721 aggaccccaa gcagatcgcg cgttcgctca agcgctccgc cgagcacagc gatcgtcgca
     3781 agagcgatcc gtatcgctcg gcgatgtcca tgctgacctt ctacatcaac cgcgccgggc
     3841 acaacctggc ggcgagccag cgcaagcgtc tcgaggacgt gaaggacgag ctgcgcgtgc
     3901 tgttccacaa gccgcccccg cgccgcagga cgacgcattg acgcgcgctc cgccgggcgc
     3961 gcgcattgcc gccctggcaa cccggggcga tgcggtgtcg cgccggcttc gaaggagcac
     4021 ggcatgtcct cactgttcag gcgccaggtg cagccggacg acgcccaggt cgaggccgcg
     4081 cgcgcggcgg ccattccgct ggacggcggc gatggggagt tcgacgagct gctgcgccgc
     4141 atcggcaacg cccgcttcgt gctgctgggc gaggcctcgc acggcacgca cgagttctac
     4201 gaggcgcgcg cccgcatcac gcggcggctg atcgccgaac gcggcttcga cgccgtcgcg
     4261 atcgagggcg actggcccga cgcctaccgc gtcaaccgcc acgtgcgcgg cctgggcgac
     4321 gatcgcgacg cctccggcgc gctggcgggc ttccagcgct ttcccacctg gatgtggcgc
     4381 aacacggccg tcgtcgactt cgtcgatcgc ctgcgcgcct ggaacgacgg ccggcccgcg
     4441 gccgcccgcg cgggcttcta cggcctggac ctgtacagcc tcaacgcgtc gatgtcgtcg
     4501 gtgctggaat acctcgaccg cgaggatccc gccgtcgcgg cgcgcgcccg cgagcgctac
     4561 gcgtgcttcg agcccttcgg cggcgacagc caggtctacg gcctgatgac cggactgggc
     4621 tcgacgccgt cgtgcgagga gggcgtgatc cgcacgctgg tcgacctgca gcaccgccgc
     4681 gcccggggcc tgcccgtcgc cggcgacgac cccgaggcgc agttcgacgc cgagcagaac
     4741 gccgcgctgg tgcgagacgc cgagcgctac taccgcggca tgtacctcag cgacgtctcg
     4801 tcctggaacg agcgcgacac ccacatggcg cgcacgctgt gggagatcga ccgccacctc
     4861 tccaggcccg gccggcccgc gaggatcgtc gtgtgggcgc acaactcgca cctgggcgat
     4921 gcctcgcaca ccgagatggg ccaggcgcgc ggcgagctca acctcgggca gctggtgcgc
     4981 gaacgcttcc cgcaggactg ctggctggtg ggcttcacga cccacgacgg cgcggtgacg
     5041 gcggcaaccg agtgggacgc gaaggccgag cacaagcgcg tgaacccgtc gcgcggcgat
     5101 tccatcgagg gcgtgctgca cgccaccggc ctgcatcgct tcctgctgcc gttccgcgat
     5161 gacccgcggc tgtcgcgtca gttcgagcat ccgctgctcg agcgcgccat cggcgtcatc
     5221 taccggcccg acaccgaacg ccagagccac tacttccacg cgcaggtggc gcggcagttc
     5281 gacgcgctga tccacgtcga ccgcacccgg gcggtcgagc cgctggaggc cgacgagccg
     5341 tgggccgcgg gccgcgcggc ccacgccggc gaagcgcccg agaccttccc gcaaggcctg
     5401 tgaggcgcgc gccttgccgc gacgcggcac gcaacgaacc aaggagcacg accatgcaat
     5461 cgcccggcca ccgcgagcat cccgagcatc acgtcatcga gagccccgtg cagggtcgcc
     5521 tgcgcgcgca gctcggcgcg gacgtgctcg ccgaatccga cgacgtcatc gaggtcgacg
     5581 aggacggcaa cccgccgcgc tactacttcc cgcgcgacag cgtgcgcatg gaccgcatga
     5641 gcgccagcga cacgcacacg tactgcccgt tcaagggcga ggccagctac ttcacgatgg
     5701 aattcctcga cggccagatg cccgacgccg cctggacgct gaaggatccc tatgacgagc
     5761 atgccgacct gaaggaccgc gtcgcgttct acagcgagaa gttcgacggc ctgcagttca
     5821 agcgcagctg aacggcctca cgctgcggcg ctcgtgcgcg cgggcgtgcg cagcgcgacg
     5881 cggaacgacg cctgcgccag cagcgccgcg cccaccagca cggcagccgc ggcgacggcc
     5941 gggtcgtgcc ccaggccggc cagcgtcgtg cacagcgcgc cgatggtcat ctgcgtgaag
     6001 ccgtacaggc ccgcggccga cccgatcagg cgcgggtcga cgctgatcga cagcgtggac
     6061 acggccgggc tgcacagccc ggcgccgacg cagtacgcga tcatcagcgc ctcgatggcg
     6121 aaggcgccgg gatgccaggc gagcagtgcg gcgagcacgg cgcccgccgc agccaggctg
     6181 gcgaggttgc ctgccatcag cagccgctcg atcgtgaagc ggccgacgag ccggctcgcg
     6241 aggatgttgc ccgtcgagac gcccaccacg agcagtccga ggtagacgcc gacctcgccg
     6301 atcggccggt gcagctcgtt gatgaagatg aagggcgcgg ccgcgatgaa gccgtagaac
     6361 gacgtggtgg cgcagccgcc gccgaacgcg aagccgacga agcgcggcga ggcgagcagg
     6421 gcgcggtagt cgcgcgcgac gccgcccgcg ctgatgtggc cgctcggctt gcccgtctcg
     6481 ggcagcagcc gccacgacac ggtgaagttc aggaccccca gcgccgtcag cagcacgaag
     6541 atcgcgcgcc agccgaacgc gccggccagc agcccgcccg ccagtggcgc gaggccggga
     6601 cccaccatcg tcatgaggct catcagcgcg agccggcgca cggcgtcgtt cgattcggcg
     6661 gtgtcgcgca cgatcgcgcg ccccagcagc agcccggcgc agccacccgc ggcctgcacc
     6721 aggcgcgcgg ccacgagcac gccgacgttg ggcgcgagcg tcgccgccag gccgcccgcg
     6781 gtgtacagcg ccaggcccgc caggagcgtg ggccggcggc cgaacacgtc cgacagcggc
     6841 ccgtagacca gctgccccac ggccaggccg aagatgtaga cgctgatcgt catccggatc
     6901 gtcgcagcgt cgacgtgcag cgactgcgcc gcgatcgtga gcgccggcac gaacatgtgc
     6961 atcgccagcg tgccgctgaa ggtgacgagc accagcagcc acaggggcac gggccggcgg
     7021 acgagcgggt aggtggcgga gatcgtgttc acgggcgcgc gggccgcacg agcaggtcgt
     7081 ggcccgaggc gatgggaagc agggcatgat gccgggcgcg attgtccgcc cgcgacgatg
     7141 cgccggcgcg ccgacgaggc atgccacccg gcaggcgccg gttcacattc agaacttgac
     7201 cgacagcccc acgttggagg tgaggtcgtt ggacgtccag gtggtgccgc gcgacacgtt
     7261 gaagtcgagc tgcgtgctcg acgacgtggc gtagctggcg ccggcggcca cgtgcgcggt
     7321 cgcgttgtcg atcacgttgt tgaaggccag cttctcgccg gacagctcga cgtagctgcg
     7381 ccagcgcgcg agcttcgtct tgtcgacggt gctgacctcc aggccggtga cgtagtgctc
     7441 gaactcgcgc ccgccgccgc gcgtgaagcc cggcgtgaag ccgatggaca ggtcttccgg
     7501 ggagtcccac atcgccgtga tgcgcagcga gcgccgcacg ttgccggcgc gcgcgggtgc
     7561 ggcgccggag acgaggtcgg cgtgcgcgtc ccacgacagg cccgacagca gcggcgcctg
     7621 gattcgatcc tgggcgctcc agcgggcgcc gaaggtcacg gcattcgggt cggcctgacg
     7681 cggcaacacg cgcaccagcg ggtcgcggtc ggggccgttg ggcgcaggca tgccgtcggc
     7741 gtggatctga acgacctggt tgctgtcgaa catcagcttc gtgggcggcg ccatcaccgc
     7801 gttcgggtcg atccgcacgg tgacgatcgc cggcttgtcc ttgcgggcgg tgatcgggac
     7861 gatcgacctc ggcttggtgt cgggcgcggc gagcacggcc gagatggcgg aacggacgat
     7921 cgtcgggtcg gcggcgtcgt tgctgggcag cagcgtggcc cagacggcgt ggtccagcgc
     7981 gtcgctgggc agcgccgcca ggtcggcgtc ggcgaccagc gcctcgtcga cgtccggggc
     8041 gtccgcggcg tcctgggcga atgccgaagc gccggccagc gcgagcaggg cggcggtgac
     8101 ggtgcggggg aggatgggac gcggggactt catggccagg gacgtgacgc agcgcagcat
     8161 aaaacaggac gcggacgacg gacaagtgcg caattgtcac gaggcgctgc gcagggccat
     8221 cgctgcaaca acggggtgtc gggcgcgcag atcgaccgca tcgcgggcag gaagttcccg
     8281 tggccgcccg ccggcagcca cggtcgcgtt ccataatcgc cacgcggtcg ctgcccaagt
     8341 gtcccccgga aggttcatcg aaccaactcc ggcccgcggg gtacccccga cggcagcgct
     8401 cttgtcatgc acgaccgagg cgatggctcc gtgtcgctgc gcgacaccac accaatcgca
     8461 tcaccagcag gttgggctgg cgaccgcacc cgaacgcctc atgagccatc gaacgaccgc
     8521 catcgccatc gctcgcgccg tgctgacgac gccggtcagc cgtttcggca tcctggagcg
     8581 cctgccgcaa tgcgtgcgct tcccgcagca ggcccatcgc gacctggtgg acgccttgtt
     8641 cgccgccggg ctggcgagcc actggacgat cgaccaggtc gccgatgtgc tggagaagga
     8701 cgacaccttc tgggcggcga tcgcggtctc gaggcaacgg ccgctcgtcc acggctggtt
     8761 catgaccaga gccggcacgc tcgccccggc gcccacgccc ttgcgcggcg tcgcacggcc
     8821 cgcgtggccc ggcgtcggca gcctggcgga cgacctgggc gtcccggtgc ccggcctgtg
     8881 gcgcctgacg cgcagcgcgt cctggcagcg ccaggcgccg ctgggccagc agcactaccg
     8941 ctacgagctg ttgccaaagc gcaccggcgg ctggcgcctg ctggaggtgc ccgagccgta
     9001 cctgcgccac ctccagcgcc aggtgctcga ccgactgctc gcccacgtgc ccccgcacga
     9061 ggccgcgtgc gcctacgtgc gcggccgttc ggtgaccgac cacgcccgcg cccacgtcgg
     9121 ccaggcggtg ctgctgaagt tcgacctgca ggatttcttc gccaccgtgc gggcctcgcg
     9181 cgtgcacgcg gcgttcgcca cgctgggcta tcccgcgccc gtcgcgcgcg tgctcgcgcg
     9241 gctgtgcacg acggccacgc ccgagccggt gctcgagcgc ctgcgcaccg agggcggctt
     9301 cgcatggacg c
//
LOCUS       DATCRO010000827         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_605546, whole genome shotgun sequence.
ACCESSION   DATCRO010000827 DATCRO010000000
VERSION     DATCRO010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605546"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VIN75_22555"
     CDS             complement(<1..98)
                     /locus_tag="VIN75_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8977014.1"
                     /translation="MDSLDLQVLSQARDWLAQGHAVWLVTVLETWGS"
     gene            complement(103..702)
                     /locus_tag="VIN75_22560"
     CDS             complement(103..702)
                     /locus_tag="VIN75_22560"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY8977015.1"
                     /translation="MELTGERLIPADRATTWAAINDPAILKDCIAGCESLERTGDDGF
                     AAVVALRVGPVSAKFKGTLRLLDVRPPEHCTLQFEAQGGAAGFGKGSADVDLSDAGPG
                     RTTLRYASKATVGGKMAQIGSRLVDAAAAKLAEDFFTAFEARLAPPASPPAEGVPSQV
                     EMSAAPAAAAVASPGRNGLAVAIVLAALLAAIAIWLSTR"
     gene            complement(693..1874)
                     /locus_tag="VIN75_22565"
     CDS             complement(693..1874)
                     /locus_tag="VIN75_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8977016.1"
                     /translation="MIAENVVHFARVLRAAGLAVGPDRVVVAVAALECVGFDRREDVR
                     AALSAVMTARHDQQPLFDAAFDAFWRDPELLGKMLMELLPKISGRGTRAREPRSNRLA
                     QALAPERDRPASPASPDASEEIRFDASFTFSDRERLQRADFETMTSDEFALASRLAEE
                     LPPPVAPVRRRRHERAPRGQADLRATLRRMVRAPDTLVPAATRPRRELPPVVVLLDVS
                     GSMDRYARVMLHYVHGLARRHARVHAFTFGTRLTDVTRCLRDRDPDVALAAVAERVGD
                     WHGGTRIAGCLAEFNRRWARRVLGASAAVLLVTDGLDRDEHGGLAEAAARLRRQAHEL
                     VWLNPLLRFDGFEPRAAGVRALLPHVDRFLPVHNLASLADLRRALQEAASPTRSSRPW
                     N"
     gene            complement(1871..>2625)
                     /locus_tag="VIN75_22570"
     CDS             complement(1871..>2625)
                     /locus_tag="VIN75_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8977017.1"
                     /translation="ELAKSLARALDARLLRVQCFEGLDVAQTAYEWNVARQMIEIRLA
                     EAAHDVDRARLAQSLYARDMLIERPLLQALTQPTSPVLLIDELDRADEPFDAFLLEVL
                     AENQLTIPEFGSVRAVAPPITLITSNRTREVHDALKRRCLYHWVGFPDIERELQILRV
                     RVPGAPQRLSRQVVEFVQRMRGLDLFKAPGVAETIDWTNALVALDAVSLDPRTVHDTL
                     GVLLKYQDDIERLRAGDSDRLLDELRAAGTLA"
BASE COUNT          360 a          945 c          994 g          326 t
ORIGIN      
        1 gagccccagg tctcgagcac cgtcaccagc cagacggcgt ggccctgcgc gagccagtcg
       61 cgcgcctggg agagcacctg caggtcgaga ctgtccatca cgtcacctcg tcgacagcca
      121 gatggcgatc gccgccagca gcgccgccag cacgatcgcc acggccagcc cgttgcggcc
      181 cgggctcgcg accgccgcgg cggccggcgc cgcgctcatc tcgacctgcg acggcacgcc
      241 ctcggccggt ggcgacgcgg gcggcgcgag gcgcgcctcg aaggcggtga agaaatcctc
      301 ggcgagcttg gccgcggcgg cgtcgacgag ccgcgagccg atctgcgcca tcttgccgcc
      361 cacggtcgcc ttggacgcgt agcgcagcgt cgtgcggccg gggccggcgt cggacaggtc
      421 gacatcggcc gaccccttgc cgaagcccgc cgcgccgccc tgggcctcga actgcagcgt
      481 gcagtgctcg ggcggccgca cgtccagcag gcgcagcgtg cccttgaact tcgcgctgac
      541 cggcccgacg cgcagcgcga cgaccgcggc gaagccgtcg tcgccggtgc gctcgagcga
      601 ctcgcagccg gcgatgcagt ccttgaggat ggccggatcg ttgatggccg cccaggtggt
      661 ggcgcgatcc gcggggatca gtcgttcgcc ggtcagttcc atggtcggct cgatcgggtg
      721 ggcgaggcgg cctcctgcag cgcgcgccgc aggtcggcca ggctcgcgag attgtgcacc
      781 gggagaaaac ggtcgacgtg cggcagcagc gcccgcacgc cggcggcgcg cggctcgaag
      841 ccgtcgaatc gcagcagcgg attgagccac acgagctcgt gcgcctggcg ccgcaagcgc
      901 gcggccgctt cggcgaggcc gccatgctcg tcgcggtcga ggccgtcggt caccagcagc
      961 acggccgcgc tcgcgcccag cacgcggcgc gcccagcgtc ggttgaactc ggccaggcaa
     1021 ccggcgatgc gcgtgccccc gtgccagtcg cccacgcgct cggcgacggc cgcgagcgcg
     1081 acgtcgggat cgcggtcgcg caggcagcgc gtcacgtcgg tcagccgcgt gccgaacgtg
     1141 aaggcatgga cgcgcgcgtg gcgccgcgcg aggccgtgca cgtagtgcag catcacccgc
     1201 gcgtagcgat ccatcgagcc cgagacgtcg agcagcacga cgaccggcgg cagttcgcgg
     1261 cgcgggcggg tggcggcggg gacgagcgtg tccggcgcgc gcaccatgcg gcgcagcgtg
     1321 gcacgcaggt cggcctggcc gcgcggggcg cgctcgtggc gccgccggcg cacgggggcg
     1381 acgggcggcg ggagttcctc ggccaggcgg ctggcgagcg cgaattcgtc ggaggtcatg
     1441 gtctcgaagt ccgcccgctg cagtcgttcg cggtcggaga acgtgaagct tgcatcgaaa
     1501 cggatctcct cgctcgcgtc cgggctcgcg ggcgaggccg ggcggtcgcg ctcgggcgcg
     1561 agcgcctgcg cgaggcggtt ggagcgcggc tcgcgcgccc gcgtgccgcg cccgctgatc
     1621 ttcggaagca gctccatcag catcttcccg agcagctcgg gatcgcgcca gaacgcgtcg
     1681 aaggcggcgt cgaacaaggg ctgctggtca tggcgagctg tcatcaccgc cgacaacgcc
     1741 gcgcgcacgt cctcgcgccg gtcgaagccg acgcattcga gcgccgcgac cgcgacgacg
     1801 acgcggtccg gccccacggc caggcccgcg gcgcgcagca cgcgggcgaa gtgcacgacg
     1861 ttctcggcga tcacgcgagc gtccctgcgg cgcgcagctc gtcgagcagg cggtccgagt
     1921 ccccggcgcg cagccgctcg atgtcgtcct ggtacttcag cagcacgccc agcgtgtcgt
     1981 gcacggtgcg cgggtccagg ctgacggcgt cgagcgcgac gagggcgttc gtccagtcga
     2041 tcgtctcggc gacgccgggc gccttgaaca ggtcgagccc acgcatgcgc tgcacgaact
     2101 cgacgacctg gcgcgacagg cgctgcggcg cgccgggcac ccggacgcgc aggatctgaa
     2161 gctcgcgctc gatgtccggg aagccgaccc agtgatacag gcagcggcgc ttgagcgcgt
     2221 cgtgcacctc gcgcgtgcgg ttgctcgtga tgagcgtgat gggcggcgcg accgcacgca
     2281 cgctgccgaa ctcggggatg gtcagctggt tctcggcgag cacctccagc aggaaggcgt
     2341 cgaagggctc gtcggcgcga tcgagctcgt cgatcagcag caccggcgac gtcggctgcg
     2401 tcagggcctg cagcagcggc cgctcgatga gcatgtcgcg cgcatacagc gactgcgcga
     2461 gccgcgcgcg gtcgacgtcg tgcgcggcct cggcgaggcg gatctcgatc atctggcgcg
     2521 cgacgttcca ctcgtaggcg gtctgcgcga cgtcgagccc ctcgaagcac tgcacgcgca
     2581 gcagccgggc gtcgagcgcg cgcgcgagcg acttcgcgag ctccg
//
LOCUS       DATCRO010000828         6672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_762717, whole genome shotgun sequence.
ACCESSION   DATCRO010000828 DATCRO010000000
VERSION     DATCRO010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762717"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VIN75_22575"
     CDS             complement(<1..1782)
                     /locus_tag="VIN75_22575"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8977018.1"
                     /translation="MSELAKSFEPAAIEARWAPLWEQAGVYEPTLDPAKPSFSIQLPP
                     PNVTGTLHMGHAFNQTIMDSLTRYHRMRGFNTLWVPGTDHAGIATQIVVERQLQDKGQ
                     TRQELGRKNFVARIWDWKQESGSTITRQMRRMGDSVSWKHEYFTMDEGLSKTVTETFV
                     RLFDEGLIYRGRRLVHWDPVLKSAVSDLEVDSKEEDGFLWLVRYPLADGSGDLTVATT
                     RPETMLGDTALMVNPADERYTALVGKEVVLPLVGRRIPVIADDYVDREFGTGVVKVTP
                     AHDANDYAVGQRHDLPIIGVLALDATINDNAPEQYRGLDRFVARKRIVADLDAQGFLV
                     ETRKHKLMVPRCERTGQVIEPMLTNQWYVAMTKPGADGTSIADKAIKAVADGSVKFHP
                     EQWVNTYNHWMGDIQDWCISRQLWWGHQIPAWYADDGRIFVARSDEEAAQKAAAAGYT
                     GKLVRDEDVLDTWYSSALVPFSTLGWPEKTIEQDLFLPSSVLVTGYDIIFFWVARMIM
                     MTTHFTGKVPFRDVYIHGLVRDAQGQKMSKSEGNVLDPVDLIDGIALEPLLDKRVVGL
                     RRPETAPKVRDNTKKEFPQGIPAYGADA"
     gene            complement(1889..2518)
                     /gene="yfcF"
                     /locus_tag="VIN75_22580"
     CDS             complement(1889..2518)
                     /gene="yfcF"
                     /locus_tag="VIN75_22580"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEY8977019.1"
                     /translation="MTAALTLFVDAQYASPYAMSAFVALDVKQLDFEVRTLDLSAAAQ
                     HAPGFAGLSLTRRVPTLLHGDFALSESSAIAEYVDEVFPGAALYPADPRRKARARQVQ
                     AWLRSDLMPIRMERSTEVVFYGAPAPALSPVAMAAAGKLFSAAEALLPDGQANLCGDW
                     CIADVDLALMLNRLVLNGDAVPARLADYARRQWRHPAVQRWVAFERPAL"
     gene            complement(2550..3917)
                     /locus_tag="VIN75_22585"
     CDS             complement(2550..3917)
                     /locus_tag="VIN75_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8977020.1"
                     /translation="MARLLMPTRRTVHAVAAIATGTAVALLVTKWRGLPDPHLALVGG
                     GLFGALVAVCVVDALLTALALRRHPIAFERQMPSAFALGQPHGLTVTLAHEGAIAWDI
                     ELFDHVPATMTQRLLPATVVLPAASRLDVRYEITPTQRGKVVFEPAAIRVRSRLGLVD
                     LQRRVGASQSVHVYPNFAALSRYAWLAGDRRLAEIGIKTYAARGAGTDFKQLGEYVPG
                     MPTRHIDWNASMRHRRPVVREFQDDRDQSVVFLLDCGRRMRADERSGTSGSHFDQALN
                     AAMLLAYVALKDGDAVGALTFGHDASEQRHFAPAKGMAALNTLVARLHDIEPSPTHSD
                     YLAAARDLMLRVRRRSLIVVLTNFRDEDCEELEDALKLMRTRHLVLLASLREGVVAEI
                     AAQPLDEPGAIVDVASAHWFQNARAEAFRRLAGKNQLLVDTEPEKLAAALVNRYHAVK
                     RARLL"
     gene            complement(3934..4920)
                     /locus_tag="VIN75_22590"
     CDS             complement(3934..4920)
                     /locus_tag="VIN75_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8977021.1"
                     /translation="MAIDSQELQFIGSALGKLRAEVGRAVVGQQEVIDQTLVAFVASG
                     HILYEGLPGLGKTLLARALAQAMSLQFSRVQFTPDLMPSDLTGHAVLEPGTDGGIGRL
                     RLRKGPIFTNLLLGDEINRAPAKTQSALLEVMQERQVTLEGQTVSLPRPFMVLATQNP
                     IDSEGTYPLPEAQRDRFLFKIDIGFPTQAEEVDVVKAATGHQVGDALPLDKVQRCLTE
                     ADVARLQQTVSAVMIDDRVVDYAVRLARATRGAVGLGNGAGSRGAIALVRAARAAALI
                     QGRDYATPDDVKRQVLPALRHRVVLSPDAQLEGRAVDDVLREIITRVDAPRL"
     gene            complement(4941..6266)
                     /locus_tag="VIN75_22595"
     CDS             complement(4941..6266)
                     /locus_tag="VIN75_22595"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY8977022.1"
                     /translation="MKGPAILRTLLVLLALLVVGWVVHKTRWVDVEVEDEAHGKAATD
                     PYYSLRHVLQGAGATLEVRNSLEPLPPAGATLFLESDQWNIFPEREARLKAWVEAGGH
                     LVLPSPHAFGDDDRLRWVPLTSIDPRRPLAKKTPAASAADDEDDDDERKDAKSDKSGR
                     TPQQTEQQRRRAEKLARRLGVRIPDRACTVFTETGATTQPAFEPGRHFRACAPAGVLR
                     TLNHVEPTWMLAGTPGTLAMRVPVGRGDVTGVTPLLELENHDLLDGDNALIAAAVLQA
                     KPGRAVWIVEDESREPLLGWLWHEARTPFLLALAAVALSLWRLMTRFGPREAVPPQAR
                     RSMGEQVRGTGQFIADTDARALHAATRKAFEDAARPRVEAWAERDDADRIAALAQSLA
                     PAAALDAAALLAALHVGGGATKPQILAATAVLEQARRAILRASAARHTS"
     gene            complement(6263..>6672)
                     /locus_tag="VIN75_22600"
     CDS             complement(6263..>6672)
                     /locus_tag="VIN75_22600"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY8977023.1"
                     /translation="LRAFERRAKAEAAVSHVRDLDVRPESLPDDVGAAAWALWQAGRV
                     PDALSLLYRGALSRLIHKYRVPISSSTTEGECLELARAGLEPPTLRYVTQVVRAWEAS
                     IYGGRALSLAMGEALCTGFRERLDGRAAAGAAA"
BASE COUNT          905 a         2358 c         2449 g          960 t
ORIGIN      
        1 cgcgtccgcg ccgtacgccg ggatgccctg ggggaactcc ttcttcgtgt tgtcgcgcac
       61 cttgggtgcc gtctcggggc ggcgcaggcc caccacgcgc ttgtccagca gcggctccag
      121 cgcgatgccg tcgatcaggt cgaccgggtc gagcacgttg ccttcggact tggacatctt
      181 ctggccctgc gcgtcgcgca ccaggccatg gatgtagacg tccctgaacg gcaccttgcc
      241 cgtgaagtgc gtcgtcatca tgatcatccg ggcgacccag aagaagatga tgtcgtagcc
      301 cgtcacgagc accgacgacg gcaggaagag gtcctgctcg atcgtcttct cgggccaacc
      361 gagcgtggag aacggcacca gggccgacga gtaccacgtg tcgagcacgt cctcgtcgcg
      421 caccagcttg ccggtgtagc cggcggccgc cgccttctgc gcggcctcct cgtcgctgcg
      481 cgccacgaag atgcggccgt cgtcggcgta ccaggccggg atctggtggc cccaccacag
      541 ttggcgcgag atgcaccagt cctggatgtc gcccatccag tggttgtacg tgttgaccca
      601 ctgctccggg tggaacttca ccgagccgtc ggccacggcc ttgatggcct tgtcggcgat
      661 ggacgtgccg tccgcgccgg gcttggtcat cgccacgtac cactggttgg tgagcatcgg
      721 ctcgatgacc tggcccgtgc gctcgcagcg cggcaccatc agcttgtgct tgcgcgtctc
      781 caccaggaag ccttgcgcgt ccaggtcggc cacgatgcgc ttgcgcgcga cgaagcggtc
      841 gagcccgcgg tactgctcgg gcgcgttgtc gttgatcgtg gcgtccagcg cgagcacgcc
      901 gatgatgggc aggtcgtggc gctggcccac ggcgtagtcg ttggcgtcgt gcgcgggcgt
      961 gaccttgacc acgccggtgc cgaactcccg gtcgacgtag tcgtcggcga tcacggggat
     1021 gcggcggccc acgagcggca gcaccacttc cttgcccacc agcgcggtgt agcgctcgtc
     1081 ggccgggttg accatcagcg cggtgtcgcc cagcatcgtc tcggggcgcg tggtggccac
     1141 ggtcaggtcg cccgagccgt cggccagcgg atagcgcacc agccacagga agccgtcctc
     1201 ctccttgctg tccacctcga ggtcggacac ggcggacttg agcaccggat cccagtgcac
     1261 gaggcggcgg ccgcggtaga tcaggccttc gtcgaacagg cgcacgaagg tctcggtgac
     1321 cgtcttcgac agcccctcgt ccatggtgaa gtactcgtgc ttccagctca ccgagtcgcc
     1381 catgcggcgc atctggcgcg tgatggtgga cccggactcc tgcttccagt cccagatgcg
     1441 tgcgacgaag ttcttgcggc cgagctcctg gcgcgtctgg cccttgtcct gcagctggcg
     1501 ctccaccacg atctgcgtgg cgatgcccgc gtggtcggtg cccggcaccc acagcgtgtt
     1561 gaagccgcgc atgcggtggt agcgcgtcaa cgagtccatg atggtctggt tgaacgcgtg
     1621 gcccatgtgc agcgtgcccg tgacgttggg cggcggcagc tggatcgaga aggacggctt
     1681 ggccggatcc agcgtgggtt cgtagacgcc cgcctgttcc cacagcggcg cccagcgggc
     1741 ttcgatggcg gcgggttcga aggacttggc gagttcggac attgaaggct cagcagtggt
     1801 ttgcagaccc cggactcgaa gggcgggcgc gcgtggcggc gcccggtgcc gggggttgcc
     1861 caattgtagg cgggccccga tggccgattc agagcgccgg cctctcgaac gccacccagc
     1921 gctgcaccgc cggatgccgc cactgccggc gcgcgtagtc ggccaggcgc gcgggcaccg
     1981 catcgccgtt caggacgagg cggttgagca tcagcgcgag atcgacgtcg gcgatgcacc
     2041 agtcgccgca caggttcgcc tggccgtcag gcagcagcgc ttcggccgcg gaaaacagct
     2101 tgcctgcggc ggccatcgcc acgggcgaca gcgcgggcgc cggcgcgccg tagaacacga
     2161 cctcggtgga gcgctccatg cggatcggca tcaggtcgct gcgcagccag gcctgcacct
     2221 ggcgcgcgcg ggccttccgg cgcggatcgg ccggatacag cgccgcgccg gggaagacct
     2281 cgtcgacgta ctcggcgatc gccgacgact ccgacagcgc gaagtcgcca tgcagcagcg
     2341 tgggcacgcg gcgcgtcagc gacaggccgg cgaatccggg cgcatgctgc gccgcggccg
     2401 acaggtccag cgtgcgcact tcgaagtcca gctgcttgac gtccagcgcc acgaaggcgg
     2461 acatcgcata cgggctggcg tactgggcgt cgacgaagag ggtgagggcg gcggtcatgg
     2521 gcgcagtgtg gcacgccccg gcacgcgtgt cagagcaggc gcgcccgctt gaccgcgtgg
     2581 tagcggttga ccagcgctgc cgcgagcttc tcgggctcgg tgtccaccag cagctggttc
     2641 ttgccggcca ggcgccggaa ggcctcggcg cgggcgttct ggaaccagtg cgccgaggcg
     2701 acatcgacga tcgcgcccgg ctcgtcgagc ggctgcgccg cgatctcggc gacgacgccc
     2761 tcgcgcaggc tcgcgagcag caccaggtgg cgcgtgcgca tcagcttgag cgcgtcctcg
     2821 agctcctcgc agtcctcgtc gcggaagttc gtcagcacca cgatcagcga gcgtcggcgc
     2881 acgcgcagca tcaggtcgcg cgcggcggca aggtagtccg aatgcgtggg gctgggctcg
     2941 atgtcgtgca ggcgcgcgac cagcgtgttg agcgcggcca tccccttggc gggcgcgaag
     3001 tggcgctgct cggatgcgtc gtggccgaag gtcagcgcgc ccacggcgtc gccgtccttg
     3061 agcgcgacgt aggccagcag catcgccgcg ttgagcgcct ggtcgaagtg gctgccgctg
     3121 gtgcccgagc gctcgtcggc gcgcatgcgg cggccgcagt ccagcaggaa cacgaccgac
     3181 tggtcgcggt cgtcctggaa ctcgcgcacc accggacggc gatggcgcat cgacgcgttc
     3241 cagtcgatgt ggcgcgtggg catgccgggc acgtactcgc ccagctgctt gaagtcggtg
     3301 ccggcgccgc gcgcggcgta ggtcttgatg ccgatctcgg ccaggcgccg gtcgcctgcc
     3361 agccaggcgt agcgcgacag cgccgcgaag ttcggataga cgtgcaccga ctgcgacgcg
     3421 cccacgcgcc gctgcaggtc gaccaggccc aggcgcgagc gcacgcggat cgccgcaggc
     3481 tcgaagacga ccttgccgcg ctgcgtcggc gtgatctcgt agcggacgtc gagcctgctg
     3541 gcggcgggca ggacgaccgt ggccggcagc aggcgctgcg tcatcgtggc gggcacgtgg
     3601 tcgaacagct cgatgtccca cgcgatcgct ccctcgtgag ccagcgtcac ggtcaggccg
     3661 tgcggctgcc cgagcgcgaa cgcactgggc atctggcgct cgaacgcgat cgggtggcgg
     3721 cgcagcgcga gcgcggtgag cagggcgtcg acgacgcaca ccgcgaccag cgcgccgaac
     3781 aggccgccac ccaccagcgc caggtgcggg tccggcaggc cgcgccactt cgtcaccagc
     3841 agcgcgacgg cggtgccggt ggcgatggcg gccaccgcat ggacggtgcg ccgggtgggc
     3901 atcaacaggc gggccacgcg cgtccttcgg gtcttacagg cgcggcgcgt cgacgcgggt
     3961 gatgatctcg cgcagcacgt cgtcgacggc gcggccttcc agctgcgcat cgggcgacag
     4021 caccacgcga tggcgcagcg ccggcagcac ctggcgcttg acgtcgtccg gcgtggcgta
     4081 gtcgcggccc tggatcagcg ccgccgcgcg cgccgcgcgc accagcgcga tcgcgccgcg
     4141 cgagcccgcg ccgttgccca ggcccaccgc gccgcgcgtg gcgcgcgcca ggcgcacggc
     4201 gtagtcgacc acgcggtcgt cgatcatcac ggcgctgacg gtctgctgaa ggcgcgcgac
     4261 gtcggcctcg gtcaggcagc gctgcacctt gtccagcggc agcgcgtcgc ccacctggtg
     4321 gccggtggcg gccttgacca cgtcgacttc ctcggcctgc gtcgggaagc cgatgtcgat
     4381 cttgaacagg aagcggtcgc gctgggcctc gggcaggggg tacgtgccct cggagtcgat
     4441 cgggttctgc gtggccagca ccatgaaggg ccgcggcagc gacacggtct ggccctccag
     4501 cgtgacctgg cgctcctgca tgacctccag cagcgcgctc tgcgtcttgg ccggcgcgcg
     4561 gttgatctcg tcgcccagca gcaggttggt gaagatcggc cccttgcgca ggcgcagccg
     4621 gccgatgccg ccgtcggtgc ccggctccag cacggcgtgg ccggtgaggt cggacggcat
     4681 caggtcgggc gtgaactgca cgcgcgagaa ctgcagggac atcgcctgcg ccagcgcgcg
     4741 cgccagcagc gtcttgccca ggcccggcag gccttcgtac aggatgtggc cggaggcgac
     4801 gaaggccacc agcgtctggt cgatgacctc ctgctggccg acgaccgcgc ggccgacctc
     4861 ggcccgcagc ttgcccagcg cgctgccgat gaattggagt tcctgggaat cgatggccat
     4921 ggtgatgtcc gtgtggaagg tcaggaggtg tggcgggcgg cggacgcgcg caggatcgcg
     4981 cgccgggcct gctcgaggac ggcggtcgcc gccaggatct gcggtttcgt ggcgccgccg
     5041 cccacgtgga gcgcggccag cagtgcggcg gcatcgaggg cggcggcggg cgccagcgac
     5101 tgcgcgagcg ccgcgatgcg gtcggcgtcg tcgcgctcgg cccaggcctc gacgcgcgga
     5161 cgggcggcgt cctcgaaggc cttgcgggtg gccgcgtgca gcgcgcgcgc gtcggtatcg
     5221 gcgatgaact ggccggtgcc gcgcacctgc tcgcccatcg agcggcgcgc ctgcggcgga
     5281 acggcctcgc gcgggccgaa gcgcgtcatc aggcgccaca gcgacagcgc caccgcggcg
     5341 agcgccagca ggaacggcgt gcgcgcctcg tgccacagcc agcccagcag cggctcgcgc
     5401 gattcgtcct cgacgatcca gaccgcgcgt cccggcttgg cctgcagcac ggccgccgcg
     5461 atcagcgcgt tgtcgccgtc cagcaggtcg tggttctcga gttccagcag cggcgtgacg
     5521 cccgtgacgt cgccgcggcc gaccggcacg cgcatggcca gcgtgccggg cgtgcccgcc
     5581 agcatccagg tgggctcgac gtggttcagc gtccgcagca cgccggcggg cgcgcaggcg
     5641 cggaagtgcc ggcccggctc gaacgcgggc tgcgtcgtgg cgccggtctc ggtgaacacc
     5701 gtgcaggcgc gatccgggat cctgacgccc agccggcgcg ccagcttctc ggcgcggcgc
     5761 cgctgctgct ccgtctgctg cggcgtccga ccggacttgt cggacttcgc gtccttgcgc
     5821 tcgtcgtcgt cgtcctcgtc gtcggcggcg gaggcggccg gcgtcttctt ggcgagcggg
     5881 cggcgcggat cgatggacgt cagcggcacc cagcgcaacc tgtcgtcgtc gccgaacgcg
     5941 tgcggcgacg gcagcacgag atggccgccg gcctcgaccc aggccttcag ccgcgcctcg
     6001 cgctcgggga agatgttcca ctggtcggac tccaggaaca gcgtcgcgcc ggccggcggc
     6061 agcggctcga gcgagttgcg cacctccagc gtggcgcccg cgccctgcag cacatggcgc
     6121 agcgagtagt acgggtcggt cgcggccttg ccgtgggcct cgtcctcgac ctcgacgtcc
     6181 acccagcgcg tcttgtgcac cacccagccg acgaccagca gggccagcag caccagcagc
     6241 gtgcgcagga tcgccggacc cttcatgccg cggcccccgc cgcggcgcgg ccgtccagcc
     6301 gctcgcgaaa gcccgtgcac agcgcctcgc ccatcgccag cgacagcgcg cggccgccgt
     6361 agatgctggc ctcccacgca cgcaccacct gcgtgacgta gcgcagcgtc ggcggctcca
     6421 ggcccgcgcg cgcgagctcg aggcattcac cctcggtggt cgaggacgag atcggcacgc
     6481 ggtacttgtg gatcagccgc gacagcgcgc cgcggtacag cagcgacagc gcgtccggca
     6541 cgcggccggc ctgccagagc gcccaggccg cggcgccgac gtcgtcgggc aggctctcgg
     6601 ggcgcacgtc gaggtcgcgc acgtggctca cggcggcctc ggccttcgca cggcgctcga
     6661 aggcgcgcag gt
//
LOCUS       DATCRO010000829         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_706720, whole genome shotgun sequence.
ACCESSION   DATCRO010000829 DATCRO010000000
VERSION     DATCRO010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706720"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1676
                     /locus_tag="VIN75_22605"
     CDS             276..1676
                     /locus_tag="VIN75_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8977024.1"
                     /translation="MTTPVQTPAARPARPAVLDLIGNTPMVELTQFDTGCCQLFLKLE
                     SQNPGGSIKDRIGLAMIETAERDGRLRPGGTVIEATAGNTGLGLALVARAKGYRVVLV
                     VPDKMSSEKVLHLKALGAEVHTTRSDVAKGHPEYYQDMAARLAREAGGDAFYIDQFNN
                     PANPLAHEMTTAPEIWQQMGHRVDAIVCGVGSGGTITGLKRYFQRVSPETEFVLADPK
                     GSVLCDYIATGRYGTAGSWAVEGIGEDFIPAIADLENVRTGYSIDDEESFATARELLK
                     REGLLAGSSTGTLLAAALRHCRAQTTPKRVVTFVCDTGTRYLSKVYNDSWMFDQGLLQ
                     RSTVGDLRDLISRRVVDEAVISVTPDDTLLTAFQRMRAADISQVPVLQDGKLAGLLDE
                     SDLLMHVSADSERFRQPVRQAMNRQLRTLRPDAGLQVLITTLQEGWVAVVADAHGFYG
                     LITRFDLLNYLRKTLA"
     gene            1683..2861
                     /locus_tag="VIN75_22610"
     CDS             1683..2861
                     /locus_tag="VIN75_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY8977025.1"
                     /translation="MSKTDKPHGFETRVIHAGQFPDPSTGAIMPPIFQTSTYVQSSPG
                     VHKGYEYSRSQNPTREALERCVADLEGGRRGYAFASGLASMSTVLELLDAGAHVIASD
                     DLYGGSFRLFERVRKRSMGLQVSFIDMSDPQAVRAAIKPNTKMVWVETPSNPLLKLID
                     LEAVAGIAREANLIAVADNTFASPWGQNPLAYGFDIVVHSTTKYLNGHSDVVGGVAVV
                     GREGRQDEWAERLAFLHNAVGAIAGPFDSFLALRGVKTLALRMERHNQSALAMAQWLE
                     SHPKVARVFYPGLESHPQFALAKRQMRGGGGMISLDLKTDLAGARRFLEHVHLFALAE
                     SLGGVESLIEHPAIMTHASIPADQRAKLGIGDTLIRLSVGIEALEDLKADLENGFAKI
                     "
     gene            3174..4943
                     /locus_tag="VIN75_22615"
     CDS             3174..4943
                     /locus_tag="VIN75_22615"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8977026.1"
                     /translation="MTKPTLHRSLLSVALGGALALLAAPAHAAPDPAVTHRFEQVVHD
                     FYERYLATHPEEATQLGDHRFDRRSGDVSAAGVAADRKLYRKTLDILQAIPASSLPPD
                     HAVDREILVNDLRARLFDIEVMKTYTWQVTDYSGTEGVYVLLARDFAPIRQRLAAAKL
                     RLEAIPRLVAAARQNLQRPPRVYTETAIAQNKGAISFVQDDLDDFIKQAPEMKAVLAP
                     ARARAAAALKDYGTWLEKTLLPRSDGDFRFGKAHFEQRLKFSLDSDLGADEILARAEA
                     DLKSTQAQMEQLALPMYQRWFPGKPTEGVDGHVIVRAVLDKIADTHPNNDTIVAEARD
                     DLDKATAFVREHRIVSLPTQPIKVIVMPEFQRGQATAFCDWAGPLEKNGETFYAISPT
                     PADWSPQRVESQYREDNSAEMRDITAHEAMPGHYLQGAHASQVKMPTLVRAMTGSGTF
                     IEGWAVYAEQIMAEAGWGGDETRMEQLKIRLRVAINAILDQKIHAGHMTEDEARHLMM
                     DTGYQEEGEAAGKWRRAEMSAGQLSTYFVGVQEQIALARDLKARMGADDLAVHDRMLS
                     HGSIATKYVRQLEGLPPADAAAK"
     gene            complement(4947..5441)
                     /locus_tag="VIN75_22620"
     CDS             complement(4947..5441)
                     /locus_tag="VIN75_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977027.1"
                     /translation="MSQSFLSVACAGLLLAAGALAAAESVDVPAVAASMAAAGPKPTA
                     TVFVTVRDAHGKVVANPGASVEAATADQAQSVGPHGVKLGVPGDVMVFVPGAMCKVTL
                     SAKMAARGVMITVDRLDSGTQCAAAEVQASAASAAGAASAASTAGGAGKDKHGGSNLL
                     DSIF"
     gene            complement(5438..6343)
                     /locus_tag="VIN75_22625"
     CDS             complement(5438..6343)
                     /locus_tag="VIN75_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977028.1"
                     /translation="MAENEAMSSLDEPEEGGEGRESTRRWLIKTLAMPAAICIVPLIF
                     ELFSHAHDERENQFRLYTELLSKREEADMAVRQAIFDKVFDKYLPPDSNDIDTRLVGL
                     ELMAANFHESVDISPLFWQLDRQVSRQREGAQRDSALAELGRIARMGKARQVEGLEIA
                     GRRQDLSYDLAVPPETLQKVSCAWSLPDVPGDRGAQHRRRLFDVELVKADLPGRRLQM
                     HLSYSDLGGDKKVHQVVVWTGLYDFPLMNFTRISDQERVAMVLTRLDPDSMSATVTFV
                     YYPSSRSGTKDKPYIDDVISRLGQP"
BASE COUNT         1039 a         2358 c         2164 g          920 t
ORIGIN      
        1 cctccacgaa ctgcatctcc gcgcccttga tctcgtagtc gaccacgtcc atcgccatct
       61 tcgctcctcc gttgaccggc catcgccggc cccgcattct ccttcaagcc gggacggcgc
      121 ccggcgcccg gccgggaccc cgggattcca ctgtccctag cacgcgcgca ttggataaag
      181 ccatgacgcg gtcgcatgac ggcgcgcgct atcctgggtc accgccaact ccgatgcgat
      241 ccggccacga gccacccgat cgccaccaaa gagacatgac cactcctgtc cagacgccgg
      301 ccgcccgccc ggcccgtccc gccgtgctcg acctcatcgg caacacgccc atggtcgagc
      361 tgacccagtt cgacaccggc tgctgccagc tgttcctcaa gctggagtcg cagaacccgg
      421 gcggatcgat caaggatcgc atcggcctcg cgatgatcga gaccgccgag cgcgacggcc
      481 ggctgcggcc cggcggcacg gtgatcgagg cgacggccgg caacacgggc ctggggctcg
      541 cgctggtcgc gcgcgccaag ggctaccgcg tcgtgctggt ggtgcccgac aagatgtcgt
      601 cggagaaggt gctgcacctg aaggcgctcg gcgccgaggt gcacaccacg cgctcggacg
      661 tcgccaaggg ccaccccgag tactaccagg acatggccgc acgcctcgcg cgcgaggccg
      721 gcggcgacgc gttctacatc gaccagttca acaaccccgc caacccgctg gcgcacgaga
      781 tgaccaccgc gccggagatc tggcagcaga tgggccaccg cgtcgacgcc atcgtgtgcg
      841 gcgtcggctc gggcggcacc atcaccggcc tgaagcgcta cttccagcgc gtctcgcccg
      901 agaccgagtt cgtgctggcc gacccgaagg gctcggtgct gtgcgactac atcgccaccg
      961 gcaggtacgg cacggccggc tcgtgggccg tcgaaggcat cggcgaggac ttcatcccgg
     1021 cgatcgccga cctggagaac gtgcgcaccg gctactcgat cgacgacgag gagagcttcg
     1081 cgaccgcgcg cgagctgctc aagcgcgagg gcctgctcgc gggctcgtcc accggcacgc
     1141 tgctggccgc cgccctgcgc cactgccgcg cgcagaccac gcccaagcgc gtggtcacct
     1201 tcgtctgcga caccggcacg cgctacctgt ccaaggtcta caacgacagc tggatgttcg
     1261 accagggcct gctgcagcgc tccaccgtcg gcgacctgcg cgacctgatc tcgcgccgcg
     1321 tcgtcgacga ggcggtcatc agcgtcacgc ccgacgacac gctgctgacc gcgttccagc
     1381 gcatgcgcgc ggccgacatc tcgcaggtgc cggtgctgca ggacggcaag ctggccggcc
     1441 tgctcgacga gtcggacctg ctgatgcacg tcagcgccga ctccgagcgc ttccgccagc
     1501 cggtgcgcca ggcgatgaac cgccagctgc gcacgctgcg ccccgacgcg ggcctgcagg
     1561 tgctgatcac gaccctgcag gagggttggg tggcggtcgt cgccgatgcg cacgggtttt
     1621 atggtctgat cacgcggttc gacctcctga actacctccg caagacactg gcataagaca
     1681 ccatgagcaa gaccgacaag ccccacggct tcgagacccg cgtcatccac gccggccagt
     1741 tccccgaccc gtcgaccggc gcgatcatgc cgccgatctt ccagacgtcc acctacgtgc
     1801 agtccagccc cggcgtgcac aagggctacg agtactcgcg cagccagaac ccgacgcgcg
     1861 aggcgctcga gcgctgcgtg gccgacctcg agggcggccg gcgcggctat gcgttcgcgt
     1921 ccgggctggc gagcatgtcg acggtgctgg aactgctgga cgcgggcgcc cacgtcatcg
     1981 ccagcgacga cctgtacggc ggctcgttcc gcctgttcga gcgcgtgcgc aagcgcagca
     2041 tgggcctgca ggtgagcttc atcgacatgt ccgacccgca ggccgtgcgc gccgcgatca
     2101 agcccaacac gaagatggtg tgggtcgaga cgccgtcgaa cccgctgctc aagctgatcg
     2161 acctggaggc ggtcgccggg atcgcgcgcg aggccaacct catcgcggtg gccgacaaca
     2221 ccttcgcgag cccgtggggc cagaacccgc tcgcctacgg cttcgacatc gtcgtgcatt
     2281 ccaccaccaa gtacctgaac ggccactccg acgtcgtcgg cggcgtggcg gtggtgggcc
     2341 gcgagggacg gcaggacgag tgggccgagc ggctcgcgtt cctgcacaac gcggtgggcg
     2401 cgatcgccgg cccgttcgac tcgttcctgg cgctgcgcgg cgtgaagacg ctcgcgctgc
     2461 gcatggagcg ccacaaccag agcgcgctgg cgatggcaca gtggctggag tcgcacccga
     2521 aggtggcgcg cgtgttctat ccgggcctgg agagccatcc gcaattcgcg ctcgccaagc
     2581 gccagatgcg cggcggcggc ggcatgatct cgctggatct caagaccgac ctcgccggcg
     2641 cccgccgctt cctggagcac gtgcacctgt tcgcgctggc cgagagcctg ggcggcgtcg
     2701 agagcctgat cgagcacccg gccatcatga cgcacgcgtc gatcccggcc gaccagcggg
     2761 cgaagctggg catcggcgac acgctgatca ggctgtcggt gggcatcgag gcgctggagg
     2821 acctgaaggc ggacctcgag aacgggttcg cgaagatctg aacgctcggc ggcgccggag
     2881 gggtccggcc tctcctcatg tcgccgccgg gctgccgcca tcgcagggca agcagccggc
     2941 gacacgaggc gaaggcccga cctcgatccc cggccgatga tcggggtcag gcctccgcct
     3001 cgaatcgcga tcagcgtcac cgccaccggc gacgcgatgg cgcgatccga ggagaggcca
     3061 gacccctcgg cttcgcgtcc tcaccaaaag acgccgcgcg accaccgcat ctgtccttcg
     3121 aacgcgccca cgggcgcgtt ttgttcgtcc aggtctgccg catacttccg gccatgacca
     3181 agcccaccct ccatcgctcg ctcctgtccg tcgcgctcgg cggcgccctc gcgctgctcg
     3241 ccgcgccggc gcatgccgcg cccgaccccg ccgtcacgca ccgattcgag caggtcgtgc
     3301 acgacttcta cgagcgctac ctcgccacgc accccgagga agccacgcag ctgggcgacc
     3361 accggttcga ccgccgcagc ggcgacgtgt ccgccgcggg cgtcgcggcc gatcgcaagc
     3421 tgtaccgcaa gacgctggac atcctgcagg cgatcccggc gtccagcctg ccgcccgacc
     3481 acgcggtcga ccgcgagatc ctcgtcaacg acctgcgcgc gcgcctgttc gacatcgagg
     3541 tgatgaagac ctacacgtgg caggtcaccg actactcggg caccgagggc gtctacgtgc
     3601 tgctcgcgcg cgacttcgcg ccgatcaggc agcgcctggc cgccgcgaag ctgcgcctgg
     3661 aggcgatccc ccggctggtc gccgccgcca ggcagaacct gcagcgcccg ccccgcgtgt
     3721 acaccgagac ggcgatcgcg cagaacaagg gcgccatctc gttcgtgcag gacgacctgg
     3781 acgacttcat caagcaggcg cccgagatga aggccgtcct cgcgcccgcc cgcgcccgcg
     3841 cggccgccgc gctgaaggac tacggcacgt ggctggagaa gacgctgctg ccgcgctcgg
     3901 acggcgactt ccgtttcggc aaggcgcact tcgagcagcg cctgaagttc tcgctcgact
     3961 ccgacctcgg cgccgacgag atcctcgccc gtgccgaggc cgacctcaag tcgacgcagg
     4021 cgcagatgga acagctcgcg ctgccgatgt accagcgctg gttcccgggc aagccgaccg
     4081 agggcgtcga cggccacgtg atcgtgcgcg ccgtgctcga caagatcgcc gacacgcacc
     4141 cgaacaacga caccatcgtc gccgaggcgc gcgatgacct cgacaaggcg accgccttcg
     4201 tgcgcgagca ccggatcgtc tcgctgccca cccagccgat caaggtcatc gtgatgcccg
     4261 agttccagcg cggccaggcc accgcgttct gcgactgggc gggtccgctc gagaagaacg
     4321 gcgagacctt ctacgcgatc tcgcccacgc cggccgactg gtcgccgcag cgcgtcgaat
     4381 cccagtaccg cgaggacaac agcgccgaga tgcgcgacat caccgcgcac gaggcgatgc
     4441 ccggccacta cctgcagggc gcgcacgcca gccaggtcaa gatgccgacg ctggtgcgtg
     4501 cgatgaccgg ctcgggcacc ttcatcgagg gctgggccgt ctacgccgag cagatcatgg
     4561 ccgaggccgg ctggggcggc gacgagacgc gcatggagca actcaagatc cgcctgcgcg
     4621 tggccatcaa cgcgatcctc gaccagaaga tccacgccgg ccacatgacc gaggacgagg
     4681 ccaggcacct gatgatggac acgggctacc aggaagaagg cgaggccgcg ggcaagtggc
     4741 ggcgcgccga gatgtcggcc ggccagctgt cgacctactt cgtcggcgtg caggagcaga
     4801 tcgcgctcgc ccgcgacctg aaggccagga tgggcgccga cgacctcgcg gtgcacgacc
     4861 ggatgctgtc gcacggctcg atcgcgacca agtacgtgcg ccagctcgag ggcctgccgc
     4921 cggccgacgc cgcggcgaag taggcgtcag aagatcgagt cgagcaggtt gctgccgccg
     4981 tgcttgtcct tgccggcgcc accggcggtc gacgcggcgg acgcagcacc ggcggccgac
     5041 gcggcggacg cctgcacctc ggcggccgcg cactgcgtgc cgctgtccag gcggtcgacc
     5101 gtgatcatca cgccgcgagc ggccatcttc gcgctgagcg tcaccttgca catggcgccg
     5161 ggcacgaaca ccatcacgtc gcccggcacg cccagcttga cgccgtgagg cccgacggac
     5221 tgggcctggt cggccgtggc ggcctcgacg ctggccccgg ggttggccac caccttgccg
     5281 tgggcgtccc gcaccgtcac gaacacggtg gccgtcggct tcgggccggc agcggccatc
     5341 gatgcggcca ccgccggcac gtcgaccgat tcggcggcgg ccagggcgcc ggcagccagc
     5401 agcaggccgg cgcaggcgac cgagaggaag gattggctca tggctgcccc agtcggctga
     5461 tgacgtcatc gatgtacggc ttgtccttgg tcccgctgcg cgagctcggg taatagacga
     5521 acgtcacggt ggccgacatc gagtcgggat ccaggcgcgt gagcaccatg gccacgcgtt
     5581 cctggtcgga gatgcgcgtg aagttcatca gcgggaagtc gtagaggccg gtccagacca
     5641 ccacctggtg caccttcttg tcaccgccca ggtcgctgta cgacaggtgc atctggagcc
     5701 gtcgtcccgg gaggtcggcc ttgaccagct cgacgtcgaa aaggcgcctc ctgtgctgcg
     5761 cgccgcggtc gccgggcacg tcgggcaggc tccaggcgca tgagaccttc tgcagcgttt
     5821 caggcggcac cgcgaggtcg tacgagagat cctgccgcct gcccgcgatc tccagcccct
     5881 cgacctgccg cgccttgccc atgcgtgcga tgcgcccgag ctccgccagc gccgaatcgc
     5941 gttgcgcgcc ttcgcgctgc cggctcacct ggcgatcgag ctgccagaac agcgggctga
     6001 tgtcgaccga ctcgtggaag ttggccgcca tcagctccag gccgaccagg cgagtgtcga
     6061 tgtcgttgga gtcgggcggc aggtacttgt cgaacacctt gtcgaagatg gcctggcgca
     6121 ccgccatgtc cgcctcctcg cgcttgctca ggagctcggt gtagagccgg aactggttct
     6181 cgcgctcgtc gtgcgcgtgc gagaacagct cgaatatcag cggcacgatg cagatcgccg
     6241 ccggcatcgc cagcgtcttg atgagccagc ggcgagtgct ctcgcgacct tcgccgccct
     6301 cctcgggctc gtccagcgac gacatggctt cgttctcggc cacggtctca gcgcctcgag
     6361 attcgggtga cgccgccgga cttggtcacc tcgtagtcgt agcgcgtggc gtcgtcgtag
     6421 acgaagacga gctcaccggg cggcggcatg tcgttgatgc gaaggtggca gcggccacgg
     6481 t
//
LOCUS       DATCRO010000830        10691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_571818, whole genome shotgun sequence.
ACCESSION   DATCRO010000830 DATCRO010000000
VERSION     DATCRO010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571818"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VIN75_22630"
     CDS             <1..1347
                     /locus_tag="VIN75_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8977029.1"
                     /translation="IQLKIEREAVRRETDEASQKRLGLIEEEIARLGREYADLEEVWT
                     AEKAAAQGSAHLKEEIDKLRFEIENLKRKGDFNKVAELQYGRLPELEKKLAEAQAVEA
                     GKAQGGDGKAPQLLRTMVGAEEIAEVVARATGIPVAKLMQGERDKLLQMEDKLHERVV
                     GQDEAIKVVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKSLAGFLFDSEDHL
                     IRVDMSEFMEKHSVSRLIGAPPGYVGYDEGGTLTEAVRRKPYSVVLLDEVEKAHPDVF
                     NVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNLGSQMIMQMAGQPSQEIRDAVWEEVK
                     GHFRPEFLNRIDETVVFHALDQANIQDIARIQLATLESRVAKMDMMLEVSPAALRELA
                     KVGFDPVFGARPLKRAIQQRIENPVARLILEGRFGPKDVIPVDVKDGEFSFGRVVH"
     gene            complement(1648..2793)
                     /locus_tag="VIN75_22635"
     CDS             complement(1648..2793)
                     /locus_tag="VIN75_22635"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8977030.1"
                     /translation="MTAPRRLLLAMTALAILSSAAGCDGQPRNLSIGVLVAQSGPSGA
                     RGKDLLDGALLAAEEINAADFRVDGRPLHVDIRATDDKGEVEPAKSQARDLIDTGIAA
                     MIGPVNSNQAAAVIPIVAARGLPQLITATSAALPALGQGNVLRLLANDDQQGRAIASF
                     AAETLAAKRIALVAESSDYGRGLNASVGAALHRLQRDAVISAQVDDKGQVAPETIARF
                     RQAGVDTIVFLAREPQLMGVLDALESSGWTDVAIVGTNVVRNRSVARRPLRVKGLYAT
                     ATAIDAREFPEGKAFLEAFRKRFDAEPVWGAQYAYDAVYALADAARQARTLDPAELVQ
                     TLKRIDPGTKVNQQMRFAASGEQVYPNIGVYKVDRGTWAMQMMSATW"
     gene            complement(2971..3900)
                     /locus_tag="VIN75_22640"
     CDS             complement(2971..3900)
                     /locus_tag="VIN75_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8977031.1"
                     /translation="MSLPLLKLASLDLVRGFVAVGRRMSITHAAEDLCLTQSAVSRQV
                     AALESHLGVRLFTRGYRAIAFTPEGERLFRSADAAVQQLQDAMGELSDAAADKPVVLG
                     ASIGVASLWLLPRLAGFQAAHPGVELRLSASDRIVDLRHDDVDLAIRYVRHAPAGTGV
                     APRRLFGETVVAVAHPSVAEGALATRRALARQTLLEFDAPRGHLRPAWLQWSSWLERR
                     GWAGAKPRGVLRFSQYDQVIHAALAGQGVALGRLPLLQPLLDEGRLVLADASRIETAH
                     GYWLLQADATPRRPVQLVADWITAEASAAAVPG"
     gene            3991..5247
                     /locus_tag="VIN75_22645"
     CDS             3991..5247
                     /locus_tag="VIN75_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977032.1"
                     /translation="MSRAAVPNPSSPSHRWWREPVAIVVAGGVVMGLSLGMRYVQGLF
                     QLPVATGRGWTRETFAFAMAIQNLAWGVAQPFTGMVADRFGSARVIAAGLALYALGLA
                     GMAGAATPAAFTWTAGVCVGVALSCTAFGTVYGALNRLLPPERRSWAMGLAGALGGFG
                     QFALVPLVQGLIGGRGWIVAVIVLALACLALLPLALLLREGAPVATEATPPSPGLRAA
                     LREAFGHRGFWLLNLGFTACGFQLAFIATHLPAYLRDKGLQPADAVAALAIIALANVA
                     GTYVFGLVGARLRRKNVLAGIYLVRSAAMALFVLLPLSPLTLHAFAAVMGFIWLGTLP
                     LTNGIVGQVFGVRHIGSLFGFVFFGHQFGSFLGVWLGGLVFVRTQSYDLVWLLAMALG
                     VLAAALHWPIDDRALARTPTRARVAA"
     gene            5244..5366
                     /locus_tag="VIN75_22650"
     CDS             5244..5366
                     /locus_tag="VIN75_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977033.1"
                     /translation="MTRARLLALAGAAAAVAALGAAWLAPDNVLALWTLVAFCG"
     gene            5498..7117
                     /gene="nadB"
                     /locus_tag="VIN75_22655"
     CDS             5498..7117
                     /gene="nadB"
                     /locus_tag="VIN75_22655"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY8977034.1"
                     /translation="MPTTASSASSPVVIVGAGLAGLTVALHLARHGRRVIVLAKRGLS
                     EGATAWAQGGIVGVLGSDDSIESHVRDTQDAGAGIVDENTARFIAENSAKAVEWLVDN
                     GVPFTADAAGPLGLHLTREGGHAVRRIAHAADATGRAIHESLLAQAQASELITLRERW
                     IAVDLVTSRHLKRDEAPRVYGIYALDIDSRRVETIAASAVVLATGGVGKVYRYTSNPD
                     TSTGDGIAMAWRAGCRAANMEFIQFHPTCLYHPQERSFLITEAMRGEGAQLKLPDGTR
                     FMFAHDERGELAPRDIVARAIDFEMKKHGIDHVWLDATHLGEAFLKEHFPTIHARCLS
                     LGIDIAKAPIPVVPAAHYTCGGMVTDLSGRSDLPGLFGVGETTYTGLHGANRLASNSL
                     LECVVIGKTCADAILADASPPVRALPEWDESQVDDADEQVVIAHNWDELRMLMWNYVG
                     IVRTTKRLERALHRIELLKTEIDEYYAHFRVTRDLLELRNLVVCAELIVRSALNRHES
                     RGLHFSRDFPRTLPVSFPTVLTRPATSREQG"
     gene            7213..8001
                     /locus_tag="VIN75_22660"
     CDS             7213..8001
                     /locus_tag="VIN75_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8977035.1"
                     /translation="MTRPAMRKAGEPPAGLDAGTVRIPIAARAFVAGWFAPGLPGRGA
                     LLLLHGVRGSRHAMTSRALFLHRMGIATLAIDLPGHGESPAPRITFGANESHGAAAAL
                     AWLAQRCAGERVGAIGVSLGAASLVLARPDPAPAAVVMESMFPTIEEAVADRLAQHVG
                     APAALLAPALLWQMPWRLGVTPAQLHPIDALPSLRSPLLIAAGDADRHTTLAETRRLF
                     EAARGDPKSLWIVPGAAHVDLHAFAREDYERRVGGFMQASLRRG"
     gene            complement(8014..8298)
                     /locus_tag="VIN75_22665"
     CDS             complement(8014..8298)
                     /locus_tag="VIN75_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEY8977036.1"
                     /translation="MDIASIHNNHEKAVFACVQETAEHFPHVRDNPDMLADIACVALN
                     RLPPRYIRHDVDFAFFQTDRERTESERAIAEAVEFAFGFVQARNAMRARA"
     gene            complement(8359..9219)
                     /gene="nadC"
                     /locus_tag="VIN75_22670"
     CDS             complement(8359..9219)
                     /gene="nadC"
                     /locus_tag="VIN75_22670"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY8977037.1"
                     /translation="MFDHDETLAEARARNVRDALYEDIGRQDWTGQLIPADKRVRAVV
                     RVRESAVLCGRDWFCSCLSTLDPTARIEWHYGEGATMSADTDVCHIEANARALLSAER
                     PALNFLQLLSGTATVTRRHVEAIEGAATSPRGCAVLDTRKTLPGLRQAQKYAVRVGGG
                     RNQRIALWHGILIKENHIAAAGGIAAVMAQAHALNAGVDIQIEVESIAQLREALAAGA
                     TSVLLDNFTLDDMREAVKISGGKAMLETSGGVTLSALRDIAATGVDRVSIGKLTKDVV
                     ATDFSMRVLD"
     gene            complement(9275..10411)
                     /gene="nadA"
                     /locus_tag="VIN75_22675"
     CDS             complement(9275..10411)
                     /gene="nadA"
                     /locus_tag="VIN75_22675"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY8977038.1"
                     /translation="MTQATYEYDRQDASGATCTASAWAKVMPTPSAHERTRLKARAKE
                     LLKRHDAVLVAHYYVDGDLQDLALETGGCVADSLEMARFGRDAPQTTLVVAGVRFMGE
                     TSKILSPGKRVLMPDLDATCSLDLGCPADDFAAFCDAHPDRTVVVYANTSAAVKARAD
                     WMVTSSCALAVVNHLKAQGRKILWAPDRHLGRYIQEQTGADMLLWNGACIVHDEFKGL
                     ELELLKKDHPDAMILVHPESPRSVVQMADVVGSTSQMLDAVVKGSAKEYIVATDNGIL
                     HRMRQLAPGKTLIEAPTAGNSATCKSCAHCPWMAMNSLQGLVDCLEQGSGAIEVAEPV
                     RGEALGCIQRMLDFVAANPAAIARPAQDKRNDPAAGLVPGIGAA"
     gene            complement(10482..>10691)
                     /locus_tag="VIN75_22680"
     CDS             complement(10482..>10691)
                     /locus_tag="VIN75_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977039.1"
                     /translation="LLEEMHRLALQPFASKTRWLFYLAAEHRGLEDLIEAREWPIRPT
                     LLHARPLTRSVTNVWNQLYEAWVGS"
BASE COUNT         1452 a         3731 c         3922 g         1586 t
ORIGIN      
        1 atccagctca agatcgagcg cgaggccgtc aggcgcgaga ccgacgaggc ctcgcagaag
       61 cgcctgggcc tcatcgagga ggagatcgcg cgactgggtc gcgagtacgc cgacctcgag
      121 gaggtgtgga cggccgagaa ggccgccgcg cagggctccg cgcacctgaa ggaggagatc
      181 gacaagctgc gcttcgagat cgagaacctg aagcgcaagg gcgacttcaa caaggtcgcc
      241 gagctgcagt acggcaggct gcccgaactc gagaagaagc tcgccgaggc gcaggccgtc
      301 gaggccggca aggcccaggg tggcgacggc aaggcgccgc agctgctgcg cacgatggtc
      361 ggcgccgagg agatcgccga ggtcgtggcg cgcgccaccg gcatccccgt ggccaagctg
      421 atgcagggcg agcgcgacaa gctgctgcag atggaggaca agctgcacga gcgcgtggtg
      481 ggccaggacg aggcgatcaa ggtcgtctcc gacgcgatcc gccgctcgcg cgcaggcctg
      541 tccgacccca accggcctct gggctcgttc ctgttcctgg gcccgaccgg cgtgggcaag
      601 accgagctgt gcaagtcgct cgcgggcttc ctgttcgaca gcgaggatca cctgatccgc
      661 gtcgacatga gcgagttcat ggagaagcac tccgtgagcc gcctgatcgg cgcgcctccg
      721 ggctacgtcg gctacgacga gggcggcacg ctcaccgagg ccgtgcggcg caagccctac
      781 agcgtggtgc tgctcgacga ggtcgagaag gcgcacccgg acgtgttcaa cgtgctgctg
      841 caggtgctgg acgacggccg cctgaccgac ggccagggcc gcaccgtgga cttcaagaac
      901 acggtcatcg tcatgaccag caacctcggc tcgcagatga tcatgcagat ggccggccag
      961 ccgtcgcagg agatccgcga cgcggtgtgg gaggaggtca agggccattt ccgccccgag
     1021 ttcctcaacc gcatcgacga gacggtcgtg ttccacgcgc tggaccaggc caacatccag
     1081 gacatcgcca ggatccagct ggccacgctc gagtcgcgcg tcgccaagat ggacatgatg
     1141 ctggaagtct cgcccgcggc gctgcgcgag ctcgccaagg tcggcttcga cccggtgttc
     1201 ggcgcgcgcc cgctcaagcg tgcgatccag cagcgcatcg agaacccggt ggcgcggctg
     1261 atcctcgagg gccgcttcgg gccgaaggac gtgatcccgg tggacgtcaa ggacggcgag
     1321 ttcagcttcg ggcgcgtggt gcactgagcg tacgtcgacc gatcgaaggc ccgacggcag
     1381 caacgccctc gggccgattc atcggcggcc cgggaggcgt ctggcctctc ctcgaattcg
     1441 cgccggggct cccgcgagtg gcctcatcgc cgatcgcgaa tcgaggcgaa ggcctgaccc
     1501 agctcgtcag cgaatgatcg gggtcaggcc tgcgctttcg ttcgcgaatc gccgagcagc
     1561 gtcgatcggc cggacgccgc gaacgaaaga caggccaggc cccttcgagc gcgtcgcgcc
     1621 ggcatgtcga acgctcgatg ccgcgcctca ccacgtcgcc gacatcatct gcatcgccca
     1681 cgtcccgcga tcgaccttgt agacgccgat gttcggatat acctgctcgc ccgacgcggc
     1741 aaagcgcatc tgctggttga ccttcgtgcc ggggtcgatg cgcttgagcg tctgcacgag
     1801 ttcggccgga tccagcgtac gggcctggcg cgcggcgtcg gccagcgcgt acacggcgtc
     1861 gtaggcgtac tgcgcgcccc acaccggctc ggcgtcgaag cgcttgcgaa aggcctcgag
     1921 gaacgccttg ccctcgggga actccctggc gtcgatggcc gtggccgtcg catacagccc
     1981 cttgacccgc agcggccggc gcgcgacgct gcggttgcgc acgacattcg tgccgacgat
     2041 ggcgacgtcc gtccagccgc tgctttccag cgcgtcgagc acgcccatca gctgcggctc
     2101 gcgcgcgagg aagacgatgg tgtcgacgcc cgcctggcgg aaccgggcga tggtctcggg
     2161 cgcgacctgg cccttgtcgt cgacctgggc ggagatgacg gcgtcgcgct gcagccggtg
     2221 cagggcggcg ccgacgctcg cgttcaggcc gcgaccgtag tcgctgcttt cggccaccag
     2281 cgcgatgcgc ttggccgcca gcgtctcggc cgcgaaggac gcgatggcgc ggccctgctg
     2341 gtcgtcgttg gccagcaggc gcagcacgtt gccctggccg agcgcgggca gggcggcgct
     2401 ggtggcggtg atcagctgcg gcaggccccg ggccgcgacg atggggatca cggcggcggc
     2461 ctggttgctg ttgaccgggc cgatcatcgc ggcgatgccg gtgtcgatca ggtcgcgcgc
     2521 ctgggacttg gcgggctcga cctcgccctt gtcgtcggtg gcccggatgt cgacgtgcag
     2581 cggccggccg tcgaccctga agtccgccgc gttgatctcc tcggcggcca gcagcgcgcc
     2641 atcgagcagg tccttgccgc gtgcgcccga gggaccggac tgcgcgacca gcacgccgat
     2701 cgacaggttc ctgggctgcc cgtcgcatcc cgcggccgac gacaggatcg ccagcgccgt
     2761 catggcgagc agcaggcggc gcggcgcggt catggagttc cttcgagtcg tcaaagggga
     2821 tgcaatgtgc ccgccgggca agtccattcc aagtcacccg gcgtctcgtc tgtaaccgga
     2881 ctttgcgcgg gaggaaatcc atcacgcaat cgcgccaagg cgtcgaaagt tcgtcagggg
     2941 ctgtcgcgat ggcgacaggc cggcatcacg tcatcccggg accgccgccg ccgaggcctc
     3001 ggcggtgatc cagtcggcga ccagttgcac ggggcgacgc ggtgtcgcgt cggcctgcag
     3061 cagccaatag ccgtgtgccg tctcgatgcg gctggcatcg gcgaggacga ggcgcccttc
     3121 gtccagcagc ggctgcagca gcggcaggcg gccgagggcg acgccctggc cggcgagcgc
     3181 ggcgtggatg acctggtcgt actggctgaa acgcagcacg ccgcgcggct tggcgcccgc
     3241 ccagccgcgg cgctcgagcc aggacgacca ctgcagccac gcggggcgca ggtgtccgcg
     3301 cggcgcatcg aactccagca gtgtctggcg ggcgagggcg cgccgcgtcg ccagtgcgcc
     3361 ctcggctacc gacgggtgcg cgacggctac caccgtctcg ccgaacagcc ggcgcggcgc
     3421 gacgccggtg cccgcgggcg cgtggcgcac gtagcggatc gcgaggtcga catcgtcgtg
     3481 acgcaggtcg acgatgcggt cgctggccga cagccgcagt tcgacgcccg ggtgcgcggc
     3541 ctggaagccg gccagccgcg gcagcagcca caggctcgcc acgccgatgc tggctccgag
     3601 cacgacgggc ttgtcggcag cggcgtcgct cagctcgccc atcgcgtcct gcagttgctg
     3661 cacggctgcg tcggcgctgc ggaacaggcg ctcgccctcg ggcgtgaacg cgatcgcgcg
     3721 gtagccacgc gtgaacagcc gcacgcccag gtgcgactcg agcgccgcca cctggcggct
     3781 gacggcggac tgcgtgaggc acaggtcctc ggcggcgtgc gtgatgctca tgcggcgtcc
     3841 gacggccacg aaaccgcgca ccaggtcgag cgaggcgagt ttgagcagag gcagtgacat
     3901 tcccgaggcg catgcgaagc ggcgaattcg tccattgaag cacaggccgg tcgatcctgt
     3961 aatgggaatc ccatgcacag gagatccgcc atgtcgcgcg ctgcggttcc gaacccttcg
     4021 tccccgtcgc accgctggtg gcgcgagccc gtcgccatcg tcgtggccgg cggcgtggtc
     4081 atggggctat cgctgggcat gcgctacgtc cagggcctgt tccagctgcc ggtggccacc
     4141 gggcgcggct ggacgcgcga gacgttcgcc ttcgcgatgg cgatccagaa cctcgcctgg
     4201 ggcgtggcgc agccgttcac gggcatggtc gccgaccgct tcggcagcgc gcgggtgatc
     4261 gcggcgggcc tcgcgctgta cgcgctgggc ctggccggca tggcgggggc tgcgacgccg
     4321 gccgcgttca cgtggacggc gggcgtgtgc gtcggcgtcg cgttgtcgtg caccgcgttc
     4381 ggcacggtgt atggcgcgct caaccgcctg ttgccacccg aacgccggag ctgggcgatg
     4441 ggcctggccg gcgcgctggg cggtttcggg cagttcgcgc tggtgcccct ggtgcagggg
     4501 ctgatcggcg gccgcggctg gatcgtcgcg gtgatcgtgc tggccctcgc ctgcctcgcg
     4561 ctgctgccgc tcgcgctgct cctgcgcgag ggcgcgcccg tcgccaccga ggcgacgccg
     4621 ccgtctcccg gcctgcgcgc cgccttgcgc gaggcgttcg gccatcgcgg gttctggctg
     4681 ctgaacctcg gcttcacggc gtgcggcttc cagctcgcct tcatcgccac gcacctgccg
     4741 gcctacctgc gcgacaaggg cctgcagccc gcggacgcgg tcgcggcgct ggcgatcatc
     4801 gcgctggcca acgtcgccgg cacctacgtg ttcgggctgg tgggcgcgcg gctgcggcgc
     4861 aagaacgtgc tggcgggcat ctacctggtg cgctcggcgg cgatggcgct gttcgtgctg
     4921 ctgccgctca gcccgctcac gctccatgcc ttcgccgcgg tgatgggctt catctggctg
     4981 ggcacgctgc cgctgaccaa cggcatcgtc ggccaggtgt tcggcgtgcg gcacatcggc
     5041 agcctgttcg gcttcgtgtt cttcggccac cagttcggct ccttcctggg cgtgtggctc
     5101 ggcggcctgg tgttcgtgcg cacgcagtcc tacgacctgg tctggctgct ggcgatggcg
     5161 ctgggcgtgc tggcggcggc gctgcactgg ccgatcgacg accgcgcgct cgcgcgcacg
     5221 cccacgcgcg cgcgggtggc cgcgtgacgc gcgcacgcct gctggcgctc gccggcgcgg
     5281 ccgcggcggt cgccgccctg ggcgcggcct ggctggcgcc ggacaacgtg ctggcgctgt
     5341 ggaccctggt ggcgttctgc ggctgacgcc agtcaagcgc ctttcacgtg gccgtggctg
     5401 gcctgcaaga gggatttgcg ccagttcgta gaatcttcgc catcgcgaag ccgtcgggcg
     5461 ccgcggcccc gcccgcaggc gaccaccacc tccttccatg cccaccactg cctcctccgc
     5521 ttccagcccc gtcgtcatcg tgggggccgg cctcgccggc ctcaccgtgg cgctgcacct
     5581 cgcgcgccac gggcggcgcg tgatcgtgct ggccaagcgc gggctcagcg aaggggcgac
     5641 ggcttgggcg cagggcggca tcgtgggcgt gctgggctcg gacgactcga tcgagtcgca
     5701 cgtgcgcgac acgcaggacg ccggcgccgg catcgtcgac gagaacaccg cgcgcttcat
     5761 cgccgaaaac agcgccaagg ccgtcgaatg gctggtggac aacggcgtgc cgttcaccgc
     5821 cgacgccgcc ggcccgctgg gcctgcacct cacgcgcgag ggcggccacg cggtgcgccg
     5881 catcgcccac gccgccgacg ccacgggccg cgcgatccac gaatcgctgc tggcgcaggc
     5941 gcaggccagc gagctgatca cgctgcgcga gcgctggatc gcggtcgacc tcgtcacctc
     6001 gcgccacctc aagcgcgacg aggcgccgcg cgtgtacggc atctacgcgc tcgacatcga
     6061 ctcgcgcagg gtcgagacca tcgccgcgtc ggccgtggtg ctggccaccg gcggcgtggg
     6121 caaggtctat cgctacacca gcaatccgga cacctccacc ggcgacggca tcgccatggc
     6181 ctggcgcgcg ggctgccgcg cggccaacat ggagttcatc cagttccacc cgacctgcct
     6241 gtaccacccg caggagcgca gcttcctcat caccgaggcg atgcgcgggg agggcgcgca
     6301 gctcaagctg ccggacggca cgcgcttcat gttcgcccac gacgagcgcg gcgagctcgc
     6361 gccgcgcgac atcgtggccc gcgcgatcga cttcgagatg aagaagcacg gcatcgacca
     6421 cgtctggctc gacgcgacgc acctgggcga agccttcctg aaggagcact tcccgacgat
     6481 ccacgcgcgc tgcctgtcgc tgggcatcga catcgccaag gcgccgatcc ccgtcgtgcc
     6541 ggccgcgcac tacacgtgcg gcggcatggt caccgacctg agcggccgct ccgacctgcc
     6601 gggcctgttc ggcgtcggcg agacgacgta caccggcctg cacggcgcca accgcctcgc
     6661 gagcaactcg ctgctggagt gcgtcgtcat cggcaagacc tgcgcggacg cgatcctggc
     6721 cgacgcctcg ccgcccgtgc gcgcgctgcc cgagtgggac gagagccagg tcgacgacgc
     6781 cgacgagcag gtcgtgatcg cgcacaactg ggacgagctg cgcatgctca tgtggaacta
     6841 cgtgggcatc gtgcgcacca ccaagcgcct ggagcgcgcg ctgcaccgca tcgagctgct
     6901 caagacggag atcgacgagt actacgcgca cttccgcgtc acgcgcgacc tgctggagct
     6961 gcgcaacctc gtcgtctgcg ccgagctcat cgtgcgctcc gcgctcaatc gccacgagag
     7021 ccgcggcctg cacttcagcc gtgacttccc gcgcacgctg ccggtgagct tcccgacggt
     7081 gctgacgcgg ccggcgacca gccgcgagca gggctgaggg cacgccgcgt gcgacgccgc
     7141 aaggtcgtcc tcggcgcggc aggggccggc gcgctcggtg cgaccggcgg ggcgctggcc
     7201 ctcggcgaag cgatgacgcg tccggcaatg cgcaaggcgg gcgagccgcc ggcgggcctg
     7261 gacgcgggca cggttcgcat cccgatcgcg gcgcgggcct tcgtcgccgg ctggttcgcg
     7321 ccgggcctgc cgggacgcgg tgcgttgctg ctgctgcacg gcgtgcgcgg cagccgtcat
     7381 gcgatgacgt cgcgcgcgct gttcctccac cgcatgggta tcgcgacgct cgcgatcgac
     7441 ctgccgggac acggcgagag ccccgcgcca cgcatcacgt tcggcgccaa cgaatcgcac
     7501 ggcgccgcgg cggccctggc gtggctcgcg cagcggtgcg cgggcgagcg cgtcggggcg
     7561 atcggcgttt cgctgggcgc cgcgtcgctg gtgctcgcac ggcccgatcc ggcgcccgcg
     7621 gccgtggtga tggagtccat gttcccgacc atcgaggagg ccgtcgccga ccggctcgcg
     7681 cagcacgtgg gcgcgccggc cgcgctgctc gcacctgcgc tgctgtggca gatgccgtgg
     7741 cgcctcgggg tgacgcccgc gcagttgcat ccgatcgacg cgctgccgtc gctgcgctcg
     7801 ccgctgctga tcgccgcggg cgacgccgac cggcacacga cgctcgcgga gacgcgccgc
     7861 ctgttcgagg ccgcccgcgg cgaccccaag tcgttgtgga tcgtgccggg cgcggcccac
     7921 gtggacctgc acgccttcgc gcgcgaggac tacgagcgcc gcgtgggcgg cttcatgcag
     7981 gcgtcgctgc gtcgcggctg aggcgcgcgc gcgtcaggcc cgcgcgcgca tcgcgttgcg
     8041 cgcctgcacg aagccgaacg cgaactcgac cgcctcggcg atggcgcgct cggactcggt
     8101 gcgctcgcgg tcggtctgga agaacgcgaa atcgacgtcg tggcggatgt agcgcggcgg
     8161 caggcggttc agggcgacgc acgcgatgtc ggccagcatg tccgggttgt cgcggacgtg
     8221 ggggaagtgt tcggccgtct cctgcacgca ggcgaagacc gctttctcgt ggttgttgtg
     8281 gatcgatgcg atgtccatcg ccgcagtgtg ccgcgcgcgg cggccgcggg ggcctgcggg
     8341 cgcgcggcaa attgccaact agtcgagcac gcgcatcgaa aaatcggtgg cgaccacgtc
     8401 cttggtcagc ttgccgatcg acacgcggtc gacgcccgtg gcggcgatgt cgcgcagcgc
     8461 ggacagcgtg acgccgcccg aggtctccag catggccttg ccgccggaga tcttcacggc
     8521 ctcgcgcatg tcgtcgaggg tgaagttgtc cagcagcacg ctggtggcgc cggcggccag
     8581 ggcctcgcgc agctgggcga tcgactcgac ctcgatctgg atgtcgacgc ccgcgttgag
     8641 cgcgtgcgcc tgcgccatca cggccgcgat gccgccggcc gccgcgatgt ggttctcctt
     8701 gatcaggatg ccgtgccaca gcgcgatgcg ctggttgcgg ccgccgccca cgcgcaccgc
     8761 gtacttctgc gcctggcgca gtccgggcag cgtcttgcgg gtgtcgagca ccgcgcagcc
     8821 gcgcgggctg gtggcggccc cctcgatggc ctcgacgtgg cgccgcgtga cggtggcggt
     8881 gcccgagagc aactgcagga agttcagcgc cgggcgctcg gccgagagca gggcgcgcgc
     8941 attggcctcg atgtggcaga cgtccgtgtc ggcgctcatc gtggcgccct cgccgtagtg
     9001 ccactcgatg cgcgccgtcg ggtcgagcgt ggacaggcag gagcagaacc agtcgcgccc
     9061 gcacaggacg gcgctctcgc gcacgcgcac gacggcgcgc acgcgcttgt cggccgggat
     9121 cagctggccg gtccagtcct ggcggccgat gtcctcgtac agggcgtcgc gcacgttgcg
     9181 cgcgcgcgcc tcggcgaggg tttcgtcgtg gtcgaacatg cgttgtctcc ggtggccgtg
     9241 cgccggactc gtgatccgcg caggctggtt cttgtcatgc ggcgccgatg ccgggcacga
     9301 ggccggcggc ggggtcgttt ctcttgtcct gcgcggggcg cgcgatcgcg gccggattgg
     9361 cggcgacgaa atccagcatg cgctggatgc agccgagcgc ctcgccgcgc accggctcgg
     9421 ccacctcgat ggcgcccgag ccctgctcga ggcagtccac gagaccctgc aggctgttca
     9481 tggccatcca ggggcagtgc gcgcagctct tgcacgtggc cgagttgccg gccgtgggcg
     9541 cctcgatcag cgtcttgccc ggcgccagct ggcgcatgcg gtgcaggatg ccgttgtcgg
     9601 tggcgacgat gtattccttg gcggagccct tgacgacggc gtccagcatc tgcgacgtcg
     9661 agccgacgac gtcggccatc tgcacgacgc tgcgcggcga ctcggggtgc accaggatca
     9721 tcgcgtccgg gtgatccttc ttcagcagtt ccagctccag gcccttgaac tcgtcgtgca
     9781 cgatgcacgc gccgttccac agcagcatgt cggcgccggt ctgctcctgg atgtagcggc
     9841 ccaggtggcg atcgggcgcc cacaggatct tcctgccttg cgccttcagg tggttgacga
     9901 ccgccagcgc gcacgaactg gtcaccatcc agtcggcgcg cgccttcacg gcggcgctgg
     9961 tgttggcgta gacgacgacg gtgcggtcgg gatgggcgtc gcagaacgcg gcgaagtcgt
    10021 cggcggggca gccgaggtcg agcgagcagg tcgcgtcgag gtcgggcatc agcacgcgct
    10081 tgccgggcga caggatcttg gaggtctcgc ccatgaagcg cacgccggcg acgaccagcg
    10141 tcgtctgcgg ggcgtcgcgt ccgaagcggg ccatctcgag cgaatcggcg acgcagccgc
    10201 cggtctcgag cgcgagatcc tgcaggtcgc cgtcgacgta gtagtgggcg acgagcacgg
    10261 cgtcgtgccg cttgaggagt tccttggccc gggccttgag ccgggtgcgc tcgtgggcgc
    10321 tgggcgtggg catcaccttg gcccaggcgc tggcggtgca ggtggcgccg gaggcgtcct
    10381 ggcggtcgta ttcgtacgtg gcttgtgtca tggcgtcagc cgccccggca gaggcggtgc
    10441 gccatcgtag ccgatggccg gaaggacgcc gggcaagagg gtcaggagcc gacccaggcc
    10501 tcgtagagct ggttccacac gttggtgacc gagcgcgtga gcggacgggc atgcagcagc
    10561 gtcgggcgga tcggccattc gcgcgcctcg atcaggtctt ccaggccgcg gtgctccgcg
    10621 gcgaggtaga acagccagcg cgtcttggac gcgaacggct gcagcgcgag gcggtgcatc
    10681 tcttccagca g
//
LOCUS       DATCRO010000831         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_661967, whole genome shotgun sequence.
ACCESSION   DATCRO010000831 DATCRO010000000
VERSION     DATCRO010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661967"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIN75_22685"
     CDS             complement(<1..314)
                     /locus_tag="VIN75_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977040.1"
                     /translation="MTSTIDVLVILALAAFGAIGAVAGAMWMKTRNAATLREASEERA
                     RLLALADYWTWETDATHKLATWRPPARRADGWKLNPPLGTPLTAIASPIPGAEGVSPG
                     TGG"
     gene            538..1008
                     /locus_tag="VIN75_22690"
     CDS             538..1008
                     /locus_tag="VIN75_22690"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8977041.1"
                     /translation="MGRTDMLTLQGENARLQKLRAMGDLNNPGIEGAARPVLDAASMA
                     ELRALDPDGKAQLVKRVLATYQASLAKLVEQLRVAHADAAWEQVGRVAHTLKSSSASI
                     GALGLSALCADIERLIRAGDAAGAQPLLEQFHAEVQRVDAAVGQTLQHLEAAAS"
     gene            1135..>2652
                     /locus_tag="VIN75_22695"
     CDS             1135..>2652
                     /locus_tag="VIN75_22695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8977042.1"
                     /translation="MTLHHGVSLEATDRRPTVLLVDDDEVNLLMTAAALRDRGFDITS
                     CTGGEEALTRLASWTPDVVVLDAIMPGLDGFETCVALRNMPGFDNVPVLMLTGLDDEA
                     SINRAYQVGATDFFVKATQWSLLAGRLRYLLRASRTRQELERSKAKLARAQDLARMGS
                     FDWWQDDNRSFVNGLELSEEGLRVFGYGPDDRVNLRDMLRMIAPTDREPLVRLARQIL
                     YQVSVIATDVPMTLPDGRFRIIHVEAEPEFDEHGHCCAYTGIVQDVTDRRIAEDRIRH
                     LANYDALTNLPNRRQLIWRAERALEFGRRLGHQVALLLIDLDRFKVINDTLGHAAGDE
                     LLMEVAFRLRGCVRHCEQLFEGVIETAGSRSHRTLEAVGRLGGDEFVALLPEVADEAD
                     AQRVATRILEALREPVVVAGQDCFATASVGIALFPRDGNTVADLLRNSDVAMYAVKSQ
                     GRNDALVYTPRLAGRGREKLALESALHKAIERDELVLYYQPKVDVANGKMVGVEAL"
BASE COUNT          391 a          944 c          943 g          374 t
ORIGIN      
        1 ccgcctgtcc caggggacac gccttccgcg cccgggatgg gcgaggcgat ggccgtcagg
       61 ggcgtgccca gcggcggatt gagcttccag ccgtcggcgc gccgcgcggg cgggcgccag
      121 gtggccagct tgtgggtggc gtcggtctcc cacgtccagt agtcggccag cgccagcagg
      181 cgggcgcgct cctcgctggc ttcgcgcagc gtggccgcgt tgcgtgtctt catccacatc
      241 gcgcccgcca cggcgccgat cgcgccgaag gcggcgagcg ccaggatcac gagcacgtcg
      301 atcgtcgagg tcatcgagtc tgcggaagtg acatgcgagc gattgtaagg agcattgatt
      361 cgcctgccgg gatgccgaag acccgggaag cgggcatccg gcttggggcc tgtagggagg
      421 tcgtgacact ttggcgccac acccgttggc gggcgtcccg attgctcggc cgctggaggg
      481 agggcggctg gctcgccaaa ggccccctgc ctgcggagta tttcgccatt gacgcccgtg
      541 gggcgcacgg acatgcttac attacagggc gagaatgctc gactacagaa actcagggcg
      601 atgggcgatt tgaacaaccc cggaatcgag ggcgcggcca ggccggtgct ggatgcggcc
      661 agcatggccg agttgcgggc gctggatccg gatggcaagg cgcagctcgt caagcgtgtc
      721 ctcgccacgt accaggcttc cctggccaag ctggtcgaac agctgcgcgt cgcgcacgcc
      781 gacgcggcct gggagcaggt gggccgcgtc gcccacaccc tcaagtcgtc gtcggccagc
      841 atcggggcgc tcggtttgtc cgcgctgtgc gccgacatcg aacgcctgat ccgcgcgggt
      901 gacgcggccg gcgcgcagcc gctgctcgag cagttccacg ccgaggtcca gcgcgtggac
      961 gctgccgtcg gccagaccct gcaacacctc gaagcggccg cctcatgagc gcgcgcacgg
     1021 ccgctccgaa gggcgctcgc gccgcagtcc gcagggcgga ggcttcctca tgaccgcgcg
     1081 cacggccgct ccgaagggcg ctcgcgccgc agtccgcagg gcggaggctt cctgatgacg
     1141 ctgcaccatg gcgtgagcct ggaggccacg gaccgccgtc cgacggtcct gctcgtcgac
     1201 gacgacgagg tcaacctgct catgaccgcc gcggccctgc gcgaccgcgg cttcgacatc
     1261 acctcctgca ccggcggcga ggaagccctc acgcgcctgg ccagctggac gcccgacgtc
     1321 gtggtgctgg acgccatcat gccgggcctg gacggcttcg agacctgcgt cgccctgcgc
     1381 aacatgcccg gcttcgacaa cgtgccggtg ctgatgctca cgggcctgga cgacgaggcc
     1441 tcgatcaacc gcgcctacca ggtcggcgcg acggacttct tcgtcaaggc cacgcagtgg
     1501 agcctgctcg ccggccgcct gcgctacctg ctgcgcgcgt cgcgcacgcg ccaggagctc
     1561 gagcgcagca aggccaagct ggcccgcgcc caggacctcg cccgcatggg ctcgttcgac
     1621 tggtggcagg acgacaaccg cagcttcgtc aacggcctgg agctgtccga ggaaggcctg
     1681 cgcgtgttcg gctacggccc ggacgaccgc gtcaacctgc gcgacatgct gcgcatgatc
     1741 gcgcccacgg accgcgagcc gctggtgcgc ctggcccgcc agatcctgta ccaggtctcc
     1801 gtcatcgcca ccgacgtgcc gatgacgctg cccgacgggc gcttccgcat catccacgtc
     1861 gaggccgagc ccgagttcga cgagcacggc cactgctgcg cctacacggg catcgtgcag
     1921 gacgtgaccg accgccgcat cgcggaagac cgcatccgcc acctggccaa ctacgacgcg
     1981 ctcacgaacc tgcccaaccg ccgccagctg atctggcgcg ccgagcgggc gctggagttc
     2041 ggccgccggc tgggccacca ggtggcgctg ctgctgatcg acctggaccg cttcaaggtc
     2101 atcaacgaca ccctcggcca cgcggccggc gacgagctgc tgatggaggt cgcgttccgc
     2161 ctgcgcggct gcgtgcgtca ctgcgagcag ctgttcgagg gcgtgatcga gacggcgggc
     2221 tcgcgctcgc accgcacgct ggaggccgtg ggccgcctgg gcggcgacga gttcgtcgcg
     2281 ctgctgcccg aagtggccga cgaggccgac gcccagcgcg tggccacccg catcctcgag
     2341 gcgctgcgcg agccggtggt ggtggcgggc caggactgct ttgccaccgc gtcggtgggc
     2401 atcgcgctgt tcccgcgcga cggcaacacg gtcgccgacc tgctgcgcaa ctccgacgtg
     2461 gcgatgtacg ccgtcaagag ccagggccgc aacgacgcgc tggtctacac gccgcgcctg
     2521 gccggccgcg ggcgcgagaa gctggcgctc gagagcgcgc tgcacaaggc catcgagcgc
     2581 gacgagctgg tgctgtacta ccagcccaag gtggacgtgg ccaacggcaa gatggtgggc
     2641 gtcgaggcgc tg
//
LOCUS       DATCRO010000832         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_447924, whole genome shotgun sequence.
ACCESSION   DATCRO010000832 DATCRO010000000
VERSION     DATCRO010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447924"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1190
                     /locus_tag="VIN75_22700"
     CDS             99..1190
                     /locus_tag="VIN75_22700"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEY8977043.1"
                     /translation="MPEPTFDSAPNDLPPPDHDAPLRPPRSSWDGGSDRSHVKTFVLA
                     ASLMAAIGLAALAVHEDVGSLWRRAFGPPLTADEMRDRIARCERDADMPCAQETLEAL
                     LKKQPDDAVARAHLGLVMAHRDDDAHAVVEFQRAIDSGEGTYDLFAWYADSLARLGRN
                     DEAIEWSYRSLSLVPNLVDVRGKLARLLVARGRPYEALSLLASYDADAAAKGRRGYFE
                     GQRIAIESALATVAPGAAGSLRLPVMAGHFYAPVALGNGKPLAFMVDTGASATTVTPE
                     MLDEARIDYRVVQPVVTMTTADGRQVAAQGITIPILRVGPYVLHDVPAVKCDHCVALL
                     GQASLSHFDLRSTRTQGVEFLTLTPRDGD"
     gene            1285..1881
                     /locus_tag="VIN75_22705"
     CDS             1285..1881
                     /locus_tag="VIN75_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8977044.1"
                     /translation="MPVSTDRKAASHARILDAASRAIRREGYGGVNVTEVMRAAQLTH
                     GGFYAHFASRDALLAEAVEQAGADIDRVLREQGDRLVAGGASPLRAFVEIYLSMAHVR
                     DCDNGCPVSLLSGEMFRQPPTVATPSRRLITQMHARVKKVLPADAPREAAWAVVSTLL
                     GAVQLARALAGSDEKAAAAVLADTRKDLLARYDSDRPQ"
     gene            1987..2691
                     /locus_tag="VIN75_22710"
     CDS             1987..2691
                     /locus_tag="VIN75_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8977045.1"
                     /translation="MKIKDSVVLVTGANRGLGLEFARQALALGAAKVYAAARDPSRVT
                     LPGMVPVKLDVTNVAEVAAAAVQCPDVTLLVNNAGIGRTGGVTGGDAATLLREHLETN
                     LFGVLHMSRALAPILARNGGGAILNILSILSWVNTPLLGTYGISKAAAWSLTNSLREE
                     LRAQGTQVVGFHGGFIDTDMIRAFDVPKSTPADVVAQSYAAIEAGETEVRVDEGTRQV
                     KAGLSQGVYLRDALTA"
     gene            2900..>3509
                     /locus_tag="VIN75_22715"
     CDS             2900..>3509
                     /locus_tag="VIN75_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8977046.1"
                     /translation="MSDTPQARPGSAPSPDEGSDALPIGARLAEFEIRQVIGAGGFGI
                     VYRAWDEALQRDVALKEYMPVSLAGRGASERVTLRSRMHEETFALGLQSFVNEARLLA
                     RFDHPALVKVFRFWEANGTAYMAMPLYKGRTLRQMRKALPAGAFDDAWMRSLIDPLLG
                     ALEVMHDAEVYHRDIAPDNILSILSWVNTPLLGTYGISKAAAW"
BASE COUNT          509 a         1311 c         1195 g          494 t
ORIGIN      
        1 gatcgggcgc gccgcccgcg tcgataatcc gcggcaaggg gagcgaggca tcgcgcgcgg
       61 cgcgcggcgc cggcccgtcc cttcaaccgt gccagccgat gcccgagccc accttcgact
      121 ccgcgccgaa cgacctgccg ccgcccgacc acgacgcccc gctgcgtccg ccgcgttcga
      181 gctgggacgg cggcagcgac cgctcccacg tcaagacctt cgtcctcgcg gccagcctga
      241 tggccgcgat cggcctggcc gcgctcgccg tgcacgagga cgtcggcagc ctctggcggc
      301 gcgcgttcgg gccgccgttg acggccgacg agatgcgcga tcgcatcgcc agatgcgagc
      361 gcgacgccga catgccctgc gcgcaggaga ccctggaggc gctgctcaag aagcagcccg
      421 acgacgccgt cgcccgcgcg cacctgggcc tggtgatggc gcaccgcgac gacgacgcgc
      481 acgcggtcgt cgagttccag cgcgcgatcg acagcggcga aggcacctac gacctgttcg
      541 cctggtacgc cgacagcctc gcgcgcctgg gacgcaacga cgaggccatc gagtggtcgt
      601 atcgctcgct gtcgctggtg cccaacctgg tggacgtgcg cggcaagctg gcgcgcctgc
      661 tggtggcgcg cggccggccc tacgaggcgc tgtcgctgct ggccagctac gacgccgacg
      721 cggcggccaa gggacgccgc ggctacttcg agggccagcg catcgcgatc gaatcggcgc
      781 tcgccacggt cgcgccaggc gcggccgggt cgctgcgcct gccggtgatg gccggccact
      841 tctacgcgcc ggtggcgctg ggcaatggca agccgctggc gttcatggtc gacaccggcg
      901 cgtcggccac caccgtcacg cccgagatgc tggacgaggc caggatcgac taccgcgtcg
      961 tccagccggt cgtgacgatg acgacggccg acggccgcca ggtcgccgcg cagggcatca
     1021 cgatcccgat cctgcgcgtg ggcccgtacg tcctgcacga cgtgccggcc gtcaagtgcg
     1081 accactgcgt cgcgctgctg gggcaggcct cgctgtcgca tttcgacctg cgctccacgc
     1141 gcacgcaggg cgtcgagttc ctcaccctga cgccgcgcga cggggactga tccaggttcc
     1201 cccgcccccg atggggacat cgccggggcg cctcggcagc cgggctgcta atatgatctc
     1261 catcatattc gagcgacttc ccccatgccc gtgtcgaccg accgcaaggc cgcctcccat
     1321 gcacgcatcc tcgacgccgc ctcgcgcgcc atccggcgcg agggctacgg cggcgtgaac
     1381 gtcacggagg tcatgcgcgc cgcacagctg actcatggcg gtttctacgc gcacttcgcc
     1441 tcgcgcgacg ccctgctggc cgaggcggtt gaacaggccg gcgccgacat cgaccgcgtg
     1501 ctgcgcgagc agggcgatcg cttggtggcc ggcggcgcga gcccgttgcg cgcgttcgtc
     1561 gagatctacc tgtcgatggc gcacgtgcgc gactgcgaca acggctgtcc cgtgtcgctg
     1621 ctgtcgggcg agatgttccg ccagccgccg accgtggcga cgccgtcgcg acggctcatc
     1681 acgcagatgc acgcccgcgt gaagaaagtg ctgcccgccg acgcgccgcg cgaggccgcg
     1741 tgggctgtcg tcagcacgtt gttgggcgcc gtgcagctgg cgcgcgcgct ggccggcagc
     1801 gacgagaagg ccgcggcagc ggtcctggcc gacacgagga aggacctgct ggcgcgctac
     1861 gacagcgatc gtccgcaata gaggcgcctc gccttccgcc acggcggatg cgcgaggatt
     1921 ttttcacccc caaatatgat tttcatcata tttcgcgacc cctcccgact ccaccaagga
     1981 aacatcatga agatcaagga ttccgtcgtc ctcgtcaccg gcgccaatcg cggcctcggt
     2041 ctggaatttg cgcgccaggc gctcgccctg ggcgccgcga aggtctatgc cgccgcgcgc
     2101 gacccgtccc gggtcacgct gcccggcatg gtgcccgtca agctcgatgt cacgaacgtg
     2161 gccgaagtcg ccgcggcggc cgtgcaatgc cccgatgtca cgctgctggt caacaacgcc
     2221 ggcatcgggc gcacgggggg cgtgactggc ggcgacgccg ccacgctgct gcgtgagcac
     2281 ctcgagacca acctgttcgg cgtgctgcac atgagccgcg cgctggcgcc catcctcgcg
     2341 cgcaacggcg gcggcgcgat cctcaacatc ctgtccatcc tcagctgggt caacacgccg
     2401 ctgctgggca cctacggcat cagcaaggcg gccgcgtggt cgctcacgaa cagcctgcgc
     2461 gaggaactgc gcgcgcaggg cacgcaggtg gtcggcttcc acggcggctt catcgatacc
     2521 gacatgatcc gcgcgttcga cgtgcccaag tccacgcccg ccgacgtcgt ggcgcagtcg
     2581 tacgcggcga tcgaagccgg cgagacggag gtccgcgtcg acgaggggac gcgccaggtc
     2641 aaggccgggc tctcgcaggg cgtctacctg cgcgacgcgc tcacggcctg acggggatgc
     2701 cggtgtcggc caagccgcgg cggcgcgcgg ctccaagggt ccggccccgt ctgcaggcgc
     2761 gcgcccgcgt cgctgctcct cagggatcac ccggacccct cgccccgacc gatgatgcga
     2821 cggaatgccg cccagtgcac cttctcgccc gctgccgcgt tgagcaggga tcgccggctg
     2881 cggcacacta cgggccacca tgtccgacac cccccaagcc cgacccggca gcgcgccttc
     2941 cccggacgag ggcagcgacg ccctgccgat cggcgccagg ctggccgagt tcgagatccg
     3001 ccaagtcatc ggcgccggcg gcttcggcat cgtctaccgg gcctgggacg aagcgctgca
     3061 gcgcgacgtc gcgctcaagg aatacatgcc ggtctcgctg gccggccgcg gcgcctccga
     3121 acgcgtgacg ctgcgctcgc gcatgcacga ggagaccttc gcgctgggcc tgcagtcctt
     3181 cgtcaacgag gcccggctgc tggcccgctt cgaccatccc gcgctcgtca aggtgttccg
     3241 cttctgggag gccaacggca ccgcctacat ggccatgccg ctgtacaagg gccgcacgct
     3301 gcgccagatg cgcaaggcgc tgccggcggg cgcgttcgac gacgcctgga tgcgctcgct
     3361 gatcgacccg ctgctcggcg cgctcgaggt gatgcacgac gccgaggtct accaccgcga
     3421 catcgcgccc gacaacatcc tgtccatcct cagctgggtc aacacgccgc tgctgggcac
     3481 ctacggcatc agcaaggcgg ccgcgtggt
//
LOCUS       DATCRO010000833         6908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_369168, whole genome shotgun sequence.
ACCESSION   DATCRO010000833 DATCRO010000000
VERSION     DATCRO010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369168"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VIN75_22720"
     CDS             complement(<1..817)
                     /locus_tag="VIN75_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY8977047.1"
                     /translation="MDATRTTATPVSAVQPVDERLPPARLAALGLQHVLVMYAGAIAV
                     PLIVGRALKLAPEQVAMLISADLLCCGLVTLIQSFGLNRWFGIRLPVMMGVTFAAVGP
                     MVAIANGVPGMDGARAIFGAGIGAGVMAILIAPLMSRLLRFFPPVVTGTTIAIIGISL
                     MRVGVGWAVGGPSNVAQFTDVPQLVAMVEAAKVEAARDAASGVATEAIASERAASVAV
                     TAAVSEAAASAPAPGRFAKMFARLKLPGPVPMNDNPAYGALDNMAVSGAVLMFI"
     gene            complement(1023..2102)
                     /gene="modC"
                     /locus_tag="VIN75_22725"
     CDS             complement(1023..2102)
                     /gene="modC"
                     /locus_tag="VIN75_22725"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY8977048.1"
                     /translation="MIEATLRLPRGAFALDVALSLPARGTSALFGPSGCGKTTLLRCL
                     AGLERAPGGRVVVEGETWQDDAAGVFVPVHRRAVGVVFQEASLFEHLDVRANVEFGWK
                     RLAPAQRRVSIEQAVEWTGIGALMGRSTAGLSGGERQRVAIARALAVSPRLLLMDEPL
                     AALDAARKAEILPAIERLNELSGITIVYVSHAISEVARLADHVVLMEAGRAVAAGPVG
                     ELLSRLDLPLPPGEDAGVVLLGEIGALDQAWHMARLDVPGASFWTRDPSLPVGHLIRL
                     RVLARDVSLSLAEQAGSSIANQIPAVVEAIADDTHPSQVLVRVRTHGGSSALLARVTR
                     RSAQALALAPGLPVWAMVKSVALLD"
     gene            complement(2099..2803)
                     /gene="modB"
                     /locus_tag="VIN75_22730"
     CDS             complement(2099..2803)
                     /gene="modB"
                     /locus_tag="VIN75_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY8977049.1"
                     /translation="MTWGDDAQAVFLTLKLASVVTVLLLALGTPLAWWLAHTRSRWKA
                     PLSALVALPLVLPPTVLGFYLLMLMGPHGAVGRATQALGIGLLPFSFAGLVVASMLYS
                     LPFVVQPIQTAFEAIGARPLEAAATLRAGPWERFAHVALPLARRGVITAAVLGFAHTV
                     GEFGVVLMIGGNIPGATRVISTQLYNHVEAIEYPEAHRLAAILLVFSFAVLLGVYRLR
                     PQGALVAAPADGAPRP"
     gene            complement(2933..3691)
                     /gene="modA"
                     /locus_tag="VIN75_22735"
     CDS             complement(2933..3691)
                     /gene="modA"
                     /locus_tag="VIN75_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8977050.1"
                     /translation="MTLDPIRGLCAVALAALAFPACADEVQVAVAANFSAAAQKIAAQ
                     FEKDTGHVVKLSFGATGKFYAQIEAGAPFDVLVAADQKTPAKLAAEGKGLPATLHTYA
                     IGKLVLWSADPALVDADGAVLRAGNWKHLSVADAKLAPYGQAARETLASLKLTDAVQP
                     RIVTAENIGQALQFVQSGNAELGFVALGQVQPPDGSRPAGSMWVVPSSLYAPIRQDVV
                     VIAATRVPKAATQFVDYLASDKAREVIKACGYGW"
     gene            complement(3688..4080)
                     /locus_tag="VIN75_22740"
     CDS             complement(3688..4080)
                     /locus_tag="VIN75_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977051.1"
                     /translation="MIDHTGVTVSDFQASKVFYVAALAAIGYEKLMEFGPEITGSVSV
                     AGFGEPPKPDFWISAGTPNRPAIHVAFRVANRAMVRAFHAAALAAGGSCNGPPGVRAH
                     YHPNYYGAFVLDPDGHNIEAVCHEPETA"
     gene            4211..5116
                     /locus_tag="VIN75_22745"
     CDS             4211..5116
                     /locus_tag="VIN75_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977052.1"
                     /translation="MSHRSRAPGPLAAIALALLAGCAAQMQPVADFGAASSHLAAAYK
                     PFTSGLGASCEQRERYIALGNPGAFDDAAAERAAAGKCKALREAGATAALFGQALSDY
                     AGALAKLSGAKPTVFDGDIKGLSGAAGALATRDGTPLFDSAKLGAATKLARAAAALLT
                     QARIETLKRSTLEDNQEPLRVVVEAMKSYAGQVYAGQLTDTREIMRGELGRLVNASNA
                     PTQADVESRLPWRWAQTAARADIAANELEVRHVRAFDKSADALLAAHADLIANFDKIG
                     GARRLELVAAFVAQVQAINDDAAAL"
     gene            5140..5592
                     /locus_tag="VIN75_22750"
     CDS             5140..5592
                     /locus_tag="VIN75_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977053.1"
                     /translation="MTDPTASDIATAALAAAAAPSDADTADAIAQLADAAGHASTVLF
                     NWRRDNPTAPDVDRALNLEMTLDQRAIDLRAKAVKLLGAQAADAIAQIEDAAQKVDDF
                     MGTVKKTGDRLAIASAVVGLAGAALVGDAGGVLQAVVSVHGAIKAAQA"
     gene            complement(5589..6005)
                     /locus_tag="VIN75_22755"
     CDS             complement(5589..6005)
                     /locus_tag="VIN75_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEY8977054.1"
                     /translation="MTTPSPQPLAAIASFHAHIYFDGPAQRETALALREQIGQRFAVA
                     LGRVHDAPIGPHARAMYQVAFDVATFGKIVPWLMLNRQGLAVLVHTNTRNERADHLSH
                     ALWMGQVLDIVGPEHLAADMDAEGPRPPNTSPSVQA"
     gene            complement(6076..6222)
                     /locus_tag="VIN75_22760"
     CDS             complement(6076..6222)
                     /locus_tag="VIN75_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY8977055.1"
                     /translation="MLYTIAVILVILWLIGMLTSVTLGGLIHILLVIAIIAVLFRLIS
                     GRGV"
     gene            complement(6244..6816)
                     /locus_tag="VIN75_22765"
     CDS             complement(6244..6816)
                     /locus_tag="VIN75_22765"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8977056.1"
                     /translation="MSKHAATFKWGIAAAAALAGLSLAGCNRPDTPADKKVTAGTELD
                     DSVITTKVKTALMTDNVGKGGDTSVETRKGEVLLSGFVDSQAQADREVQLAKSVDGVQ
                     SVQNKLMVKDGKSTAGNVLDDSVVTVKVKSALMADDRTRGTQFAVTTHKGVVQLSGFV
                     DSADEQARATTVARNVEGVQSVVNDTSIKK"
BASE COUNT         1017 a         2515 c         2460 g          916 t
ORIGIN      
        1 ggatgaacat cagcaccgcg ccggacaccg ccatgttgtc gagcgcgccg taggccgggt
       61 tgtcgttcat cggcaccggg ccgggcagct tcagcctcgc gaacatcttc gcgaagcggc
      121 ccggcgcggg cgccgacgcg gcggcctcgg acaccgccgc cgtcaccgcc accgaggcgg
      181 cgcgctcgga cgcgatcgcc tcggtcgcga caccggaggc cgcgtccctg gccgcctcga
      241 ccttggctgc ctcgaccatc gccaccagct gcggcacgtc ggtgaactgc gccacgttgg
      301 acgggccgcc caccgcccag cccacgccga cgcgcatcag gctgatgccg atgatcgcga
      361 tggtcgtgcc ggtgaccacc ggcgggaaga agcgcagcag ccggctcatc agcggcgcga
      421 tgaggatggc catgacgccc gcgccgatgc cggcgccgaa gatggcgcgc gcgccgtcca
      481 tgccgggcac gccgttggcg atggccacca tcgggccgac ggcggcgaag gtcacgccca
      541 tcatcacggg cagccggatg ccgaaccagc ggttcaggcc gaacgactgg atcagcgtga
      601 cgaggccgca gcacagcagg tcggccgaga tcagcatcgc cacctgctcg ggcgcgagct
      661 tgagcgcgcg cccgacgatc agcggcaccg cgatcgcgcc ggcgtacatg accagcacgt
      721 gctgcaggcc cagcgcggcg aggcgcgcgg gcggcaggcg ctcgtccacg ggttgaacgg
      781 cggagacagg cgttgctgtc gtgcgggtgg cgtccatcgc gtcctccagg gtgaagaggg
      841 cgcaggcctt gaaggcgcat gcaccctccc ctcaccacag cgggctctgt aaggagtgga
      901 cgtcgcaacc gccgaccgga ccgggcccgg cgcgacgggc accgcggcga cgtgggaaaa
      961 ctgtatacag tctggaggat gctctgtata cgggctaacc cgatcctatc gggcggctga
     1021 cgtcaatcca gcagcgcgac cgacttgacc atcgcccaca ccggcagccc cggcgcgagc
     1081 gcgagcgcct gcgcgctgcg gcgcgtcacg cgcgcgagca gcgcgctgga gccgccgtgc
     1141 gtgcgcacgc gcacgagcac ctgcgaggga tgcgtgtcgt cggcgatggc ctcgacgacc
     1201 gcggggatct ggttggcgat gctgctgccc gcctgctccg cgaggctcag cgagacgtcg
     1261 cgcgcgagca cgcgcagccg gatcaggtgg cccacgggca gcgacgggtc gcgcgtccag
     1321 aacgacgcgc ccgggacgtc caggcgcgcc atgtgccagg cttggtcgag cgcgccgatc
     1381 tcgcccagga ggacgacgcc tgcgtcctcg ccgggcggca gcggcaggtc cagccggctc
     1441 aggagctcgc ccaccggacc cgcggcgacg gcgcggccgg cctccatcag cacgacgtga
     1501 tcggccaggc gcgcgacttc ggagatcgca tggctgacgt agacgatggt gatgcccgac
     1561 agctcgttca ggcgctcgat cgccggcagg atctcggcct tgcgggcggc gtcgagcgcc
     1621 gccagcggct cgtccatcag caaaaggcgc gggctgacgg ccagcgcgcg cgcgatcgcc
     1681 acgcgctggc gctcgccgcc ggacaggccc gcggtcgagc ggcccatcaa cgcgccgatc
     1741 ccggtccatt cgacggcctg ctcgatggac acgcggcgct gcgccggcgc cagccgcttc
     1801 cagccgaatt cgacgttggc gcgcacgtcc aggtgctcga acaggctggc ctcctggaac
     1861 acgacgccca ccgcgcgccg atgcacgggc acgaacacgc ccgcggcgtc gtcctgccag
     1921 gtctcgccct cgacgacgac gcgtccgccg ggcgcgcgct ccaggcccgc gaggcagcgc
     1981 agcagcgtcg tcttgccgca gcccgagggc ccgaacagcg cgctggtgcc gcgcgcgggc
     2041 agggacagcg ccacgtccag cgcgaacgcc ccgcgcggca gccgcagcgt cgcctcgatc
     2101 atgggcgcgg cgctccgtcg gccggcgccg ccaccagcgc gccctgcggc cgcagccggt
     2161 agacgcccag cagcacggcg aagctgaaca ccagcaggat cgcggccagc cggtgcgcct
     2221 cggggtactc gatcgcctcg acgtggttgt acagctgcgt cgagatgacg cgcgtcgcgc
     2281 cggggatgtt gccgccgatc atcaggacga cgccgaactc gcccaccgtg tgcgcgaagc
     2341 ccagcaccgc cgcggtgatc acgccgcgcc gcgcgagcgg cagcgccacg tgcgcgaagc
     2401 gctcccacgg gccggcgcgc agcgtggcgg cggcttccag cggccgcgcg ccgatcgcct
     2461 cgaacgcggt ctggatcggc tgcaccacga acggcagcga gtaaagcatc gacgccacga
     2521 cgagccccgc gaagctgaag ggcagcaggc cgatgcccag cgcctgcgtc gcacggccga
     2581 cggcgccgtg cgggcccatc agcatcagca ggtagaagcc cagcaccgtg ggcggcaaca
     2641 cgagcggcag cgcgacgagc gccgacagcg gcgccttcca gcgcgatcgc gtgtgcgcca
     2701 accaccacgc gagcggcgtg cccagcgcga gcagcagcac ggtgacgacg gacgcgagct
     2761 tgagcgtcag gaagaccgcc tgtgcatcgt cgccccaggt caatggagtc cctcccgcgc
     2821 cgggcacggc gctccgtcca ccgagtgggc cgcgggccgg cctggggcgg cccggcgccc
     2881 ggcccgcgag cgggttgtcg ctgcttgacg catcgctgtc acgcccgacc cgtcaccagc
     2941 cgtagccgca ggccttgatc acctcgcgtg ccttgtcgct cgcgaggtag tccacgaact
     3001 gcgtggcagc cttgggaacc ctggtggcgg cgatgacgac gacgtcctgc cggatcggcg
     3061 cgtagaggct ggacggcacc acccacatcg agcctgccgg cctggagccg tcgggcggct
     3121 gcacctggcc cagcgcgacg aagcccagct ccgcgttgcc gctctgcacg aactgcagcg
     3181 cctggccgat gttctcggcg gtgacgatgc gtggctgcac ggcgtcggtg agcttgagcg
     3241 acgccagcgt ctccctggcc gcctggccgt agggcgcgag cttggcgtcg gcgaccgaca
     3301 ggtgcttcca gttgccggcc ctgagcaccg cgccgtcggc atcgaccagc gccggatcgg
     3361 ccgaccacag caccagcttg ccgatcgcgt aggtgtgcag cgtcgcaggc aggcccttgc
     3421 cctcggcggc cagcttggcg ggcgtcttct ggtcggctgc caccagcacg tcgaacggcg
     3481 cgccggcctc gatctgcgcg tagaacttgc ccgtcgcgcc gaacgacaac ttgaccacgt
     3541 ggccggtgtc cttctcgaac tgcgcggcga tcttctgcgc ggcggccgag aagttggcgg
     3601 cgacggccac ctgcacctcg tcggcgcagg ccggaaaggc gagggcggcg agggccacgg
     3661 cgcacaggcc gcggatcggg tcaagcgtca tgcggtctcc ggttcatggc agacggcctc
     3721 gatgttgtgg ccgtccgggt cgagcacgaa ggcgccatag tagttcgggt ggtaatgggc
     3781 ccggacgccc ggggggccgt tgcagctgcc acccgccgcc agcgccgccg cgtggaacgc
     3841 gcgcaccatc gcgcggttcg cgacgcggaa cgcgacgtgg atggccggcc ggttgggcgt
     3901 gccggcgctg atccagaagt cgggcttggg cggctcgccg aagccggcca cggacaccga
     3961 gccggtgatc tcggggccga actccatcag tttctcgtag ccgatggcgg ccagcgccgc
     4021 gacgtagaag accttgctgg cctggaagtc gctgacggtc acgccggtgt ggtcgatcat
     4081 cgcgcagtcc tcctgaggtg atgggcgatt gtgcggcatg cccgccatcc ccccaggacg
     4141 agcggtgcgc cgggccgcgc gcgatcgtag actgcccgcc acacccttcg aacaggcgca
     4201 ggagatgccg atgtcccacc gttcgcgcgc ccccgggccg ctcgccgcga tcgcgctcgc
     4261 cctgctggca ggctgcgccg cccagatgca gccggtggcc gacttcggcg ccgcgtccag
     4321 ccacctggcc gcggcctaca agccgttcac gtcgggcctg ggcgccagct gcgagcagcg
     4381 cgaacgctac atcgcgctgg gcaaccccgg cgcgttcgac gatgcggccg ccgagcgcgc
     4441 cgcggccggc aagtgcaagg ccttgcgcga ggccggcgcc accgcggcgc tgttcggcca
     4501 ggcgctgtcg gactacgccg gcgcgctggc caagctgtcg ggcgccaagc cgacggtgtt
     4561 cgacggcgac atcaagggcc tgtccggcgc cgccggcgcg ctcgccacgc gcgacggcac
     4621 gcccttgttc gacagcgcca agctcggcgc cgccaccaag ctcgcgcgcg ccgcggcggc
     4681 gctgctcacg caagccagga tcgagacgct caagcgctcg acgctggaag acaaccagga
     4741 gccgctgcgc gtcgtcgtcg aggcgatgaa gtcgtatgcc ggccaggtct acgcgggcca
     4801 gctcaccgac acgcgcgaga tcatgcgcgg cgagctgggc cgcctcgtca acgcatccaa
     4861 tgcgccgacg caggccgacg tcgagtcgcg cctgccctgg cgctgggcgc agactgcagc
     4921 ccgcgccgac atcgcggcca acgagctcga ggtgcgccac gtgcgcgcgt tcgacaagag
     4981 cgccgacgcg ctgctggccg cccacgccga cctgatcgcc aacttcgaca agatcggcgg
     5041 cgcgcgccgc ctcgagctgg tcgcggcttt cgtcgcccag gtgcaggcga tcaacgacga
     5101 cgccgcggca ttgtgaccca gagcttcgag aggcccgcca tgaccgaccc caccgcatcc
     5161 gacatcgcca ccgccgccct cgccgccgcg gccgcgccgt ccgacgccga caccgccgac
     5221 gccatcgcgc agctggccga cgccgccggc cacgcgtcga cggtgctgtt caactggcgc
     5281 cgcgacaacc ccacggcgcc cgacgtcgac cgcgcgctca acctggagat gacgctcgac
     5341 cagcgcgcca tcgacctgcg cgccaaggcc gtcaagctgc tcggcgcgca ggccgccgac
     5401 gccatcgcgc agatcgagga cgcggcgcag aaggtcgacg atttcatggg cacggtcaag
     5461 aagaccggag accgcctggc catcgccagc gcggtggtgg gcctggccgg cgccgccctc
     5521 gtgggcgatg cgggcggcgt gctgcaggcc gtggtctcgg tgcacggcgc catcaaggcc
     5581 gcgcaagcct aggcttgcac cgacgggctc gtgttgggcg ggcgcgggcc ttccgcgtcc
     5641 atgtccgcgg ccaggtgctc cgggccgacg atgtccagca cctggcccat ccacagcgca
     5701 tgcgacaggt ggtcggcgcg ctcgttgcgc gtgttcgtgt gcaccagcac ggccaggccc
     5761 tggcggttga gcatcagcca cgggacgatc ttgccgaagg tcgcgacgtc gaacgccacc
     5821 tggtacatcg cgcgggcgtg cggtccgatc ggcgcgtcgt gcacgcggcc cagcgccacg
     5881 gcaaagcgct ggccgatctg ctcgcgcagc gcgagcgcgg tctcgcgctg ggcggggccg
     5941 tcgaagtaga tgtgcgcgtg gaagctcgcg atggcggcga gcggctgggg cgagggcgtc
     6001 gtcatgcccg cactgtaaga cggccccggc cgcgatgtcg cgacaggggc cgttctctcc
     6061 gatgggaggg tcgcgtcaga cgccgcgccc gctgatcagg cggaacagca ccgcgatgat
     6121 cgcgatcacg agcaggatgt ggatcaggcc gcccagcgtc accgaggtga gcatgccgat
     6181 gagccaaagg atgacgagga tgacggcgat ggtgtagagc atggttttct cctgtttgtc
     6241 cgactacttc ttgatgctgg tgtcgttgac gacgctctgg acgccctcga cgttgcgtgc
     6301 gacggtcgtc gcgcgggcct gctcatcggc gctgtcgacg aagccgctca gctgcacgac
     6361 gcccttgtga gtggtcacgg cgaattgcgt gccacgcgtg cggtcgtcgg ccatcagcgc
     6421 cgacttcacc ttgacggtca cgaccgagtc gtccagcacg ttgccggccg tcgacttgcc
     6481 gtccttgacc atcagcttgt tctgcaccga ctggacgccg tccacggatt tggccagctg
     6541 cacctcgcga tcggcctgcg cctgcgagtc gacgaagccg ctcaggagca cctcgccctt
     6601 gcgcgtctcg acgctggtgt cgccgccctt gccgacgttg tcggtcatca acgcggtctt
     6661 gaccttggtg gtgatgacgg agtcgtcgag ctcggtgccg gccgtcacct tcttgtctgc
     6721 gggagtgtcc gggcggttgc aaccggccag cgacaggccg gcgagcgccg ccgcggcggc
     6781 gatgccccat ttgaacgttg ctgcgtgctt gctcatgttc gagtctccag cttggcctgc
     6841 ggcccctggg gaaggttcag caagcaccgt gcccgacagt gacaccctgc gcggccgcat
     6901 gtttcagc
//
LOCUS       DATCRO010000834         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_875362, whole genome shotgun sequence.
ACCESSION   DATCRO010000834 DATCRO010000000
VERSION     DATCRO010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875362"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VIN75_22770"
     CDS             complement(<1..1527)
                     /locus_tag="VIN75_22770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY8977057.1"
                     /translation="MEQTPLAFSGGDGGGDALTLAHYAERAYLEYALSVVKGRALPDV
                     TDGCKPVQRRILYTMHRMGLSFTNASAAGPAPRKSAKVVGDVLGNFHPHGDTAAYDAL
                     VRMAQDFSLRYPLIDGQGNFGSRDGDGAAAMRYTEARLNPIARLLLDEIDEGTVAFMP
                     NYDGTTTEPKQLPARLPFVLLNGASGIAVGLATEVPSHNLREVAEAAIAMIRNEKLSD
                     DELFAALPGPDFPGGGQIISSEADIRDAYVTGRGSLKVRARWKIEELARGQWQLVVTE
                     LPPGASTQRVLEEIEELTNPKIKAGKKALTAEQLQLKTTVLAVLDGARDESDREASVR
                     LVFEPKSRAIEQQDLINTLLAHTSLESSAPINLTMIGGDGRPTLKSLRQILAEWVAFR
                     QHTVKLRTEHRLRNVLDRIHILEGRQLVLLNIDRVIEIIRHSDEPKPALIAEFRLSDR
                     QADDILDIRLRQLARLEAIKIEQQLAEFRDQQKKLEGILESPAALKRTVIKEIEADAK
                     A"
     gene            1526..1648
                     /locus_tag="VIN75_22775"
     CDS             1526..1648
                     /locus_tag="VIN75_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977058.1"
                     /translation="MVKPEFLCSLAGADDGACCGPRDAIVGVHAPAAGAEPQIS"
     gene            complement(1709..>2355)
                     /locus_tag="VIN75_22780"
     CDS             complement(1709..>2355)
                     /locus_tag="VIN75_22780"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8977059.1"
                     /translation="PVLWGYVDGAGIAHFASSQVDGRYEQVLRETDGRRVAGKTQGTD
                     GLLTWLEISPDVKALQPVLDEAARATGVDEELLKAVITVESNYNPRAVSPRGAVGLMQ
                     LTPVTADRYGTREERQTPASRRLLNARTNVFTGARMLADLTRRYGGIDIALAAWNAGE
                     GTVRKAGGQMPDIAETEAHVHMVLELYWALLQRKLPRHAHGVEVASCAPQTCGQ"
BASE COUNT          291 a          785 c          854 g          425 t
ORIGIN      
        1 ggccttggcg tcggcctcga tctccttgat gacggtgcgc ttgagggcgg cggggctttc
       61 gaggatgcct tcgagcttct tctgctggtc gcggaactcg gccagctgct gctcgatctt
      121 gatcgcctcg agacgcgcca actggcgcag ccggatgtcg aggatgtcgt cggcctggcg
      181 atcgctgaga cggaactcgg cgatcagcgc gggcttgggc tcgtcactgt ggcggatgat
      241 ctcgatcacg cgatcgatgt tgagcagcac cagctgccgg ccttcgagaa tgtggatgcg
      301 atcgagcacg ttgcgcagcc ggtgctcggt gcgcagcttg acggtgtgct ggcggaacgc
      361 cacccactcg gcgaggatct ggcgcaggct cttgagcgtg ggccggccgt cgccgccgat
      421 catcgtgagg ttgatcggcg cggagctctc caggctggtg tgggccagca gcgtgttgat
      481 caggtcctgc tgctcgatcg cgcggctctt gggctcgaag accaggcgca cggacgcctc
      541 gcgatccgac tcgtcgcgcg cgccatcgag cactgcgagg accgtcgtct tgagctgcag
      601 ctgttccgcg gtcagcgcct tcttgcccgc cttgatcttg ggattcgtga gttcctcgat
      661 ctcctccagc acgcgctgcg tgctcgcgcc gggaggcagc tcggtgacga ccagctgcca
      721 ctggccgcgc gccagttcct cgatcttcca gcgcgcccgc accttcaggc tgcctcgacc
      781 cgtgacgtag gcgtcgcgga tgtcggcttc gctggaaatg atctggccgc cgccggggaa
      841 gtcggggccg ggcagtgcgg caaacagctc gtcgtccgag agcttctcgt tcctgatcat
      901 cgcgatggcg gcctcggcca cttcgcgcag gttgtggctg ggcacctccg tggccaggcc
      961 caccgcgatg ccgctggcgc cgttgagcag cacgaatggc aggcgcgccg gcagttgctt
     1021 gggctcggtc gtcgtgccgt cgtagttcgg catgaaggcg acggtgccct cgtcgatctc
     1081 gtcgagcagc aggcgcgcga tcgggttcag gcgcgcctcg gtgtagcgca tcgcggcggc
     1141 gccgtcgccg tcgcggctgc cgaagttgcc ctggccgtcg atcagcggat agcgcaggct
     1201 gaagtcctgc gccatgcgca cgagcgcgtc gtaggcggcg gtgtcgccat gcgggtggaa
     1261 gttgcccagc acgtcgccca ccaccttggc gctcttgcgc ggcgccgggc ccgcggcgct
     1321 ggcgttggtg aacgacagcc ccatgcggtg catggtgtag aggatgcggc gctgcacggg
     1381 cttgcagccg tcggtcacgt cgggcagcgc acggcccttg acgacgctca gcgcgtattc
     1441 gaggtaggcg cgttccgcat agtgggcgag ggtcagcgcg tcgccgccac cgtcgccgcc
     1501 gctgaacgcg aggggagtct gttccatggt caagccggag ttcctttgtt cgctcgcggg
     1561 cgccgacgac ggcgcgtgct gcggcccgcg ggacgccatt gtcggcgttc acgcccccgc
     1621 ggccggtgcc gaaccgcaaa tttcctgagg atttgtctgg ccggtgacac ccggcaggcg
     1681 cttcagggct cgcgcggtga gcctgcgctc actggccgca ggtctgcggg gcgcaggacg
     1741 ccacttcgac gccgtgggcg tggcgcggca gcttgcgctg caacagggcc cagtagagct
     1801 cgagcaccat gtggacgtgg gcttcggtct cggcgatgtc gggcatctgg ccgccggcct
     1861 tgcgcaccgt gccttcgccc gcgttccagg ccgccagcgc gatgtcgatg ccgccgtagc
     1921 gccgcgtcag gtcggccagc atgcgtgcgc cggtgaagac gttggtgcgg gcattcagca
     1981 ggcgccggct ggccggcgtc tggcgttcct cgcgggtgcc gtagcggtcg gccgtcaccg
     2041 gcgtcaattg catcaggccg accgcgccgc gcggcgagac cgcgcgcggg ttgtagttcg
     2101 attcgacggt gatcaccgcc ttgagcagtt cctcgtcgac gccggtggcg cgcgcggcct
     2161 cgtccagcac cggctgcagc gccttcacgt cgggcgagat ctcgagccag gtcagcaggc
     2221 cgtcggtgcc ctgcgtcttg cctgccacgc ggcggccgtc ggtctcgcgc agcacctgct
     2281 cgtagcggcc gtcgacctgg ctgctggcga aatgcgcgat gccggcgccg tcgacgtagc
     2341 cccacagcac cggtt
//
LOCUS       DATCRO010000835         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_650674, whole genome shotgun sequence.
ACCESSION   DATCRO010000835 DATCRO010000000
VERSION     DATCRO010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650674"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VIN75_22785"
     CDS             complement(<1..994)
                     /locus_tag="VIN75_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8977060.1"
                     /translation="MNLSSPFVRRPIATVLLTAGVALAGIAGFFVLPVAPLPQVDYPA
                     ISVSASIPGASPDTMANSVATPLERRLGTISGVNEITSNSGTGSTRVSLQFDLNRDID
                     GAAREVQAAINAARVDLPATLKSNPTYRKANPSDAPCIILALTSRTRTPQQIYDAVSN
                     IVQQKVAQVQGVGDVEIGGGSLPAVRIDVNLLALNDLGIPAEDVRAAIQASNATRPKG
                     SIEDGVQRLQIYTRQPGHTAAEYRNLTVAWRNGAAVKLSDIANVTDGPEDVRTLGLFN
                     GSPAVIVIVTRQPGANIIETVNGVRALVPELKAQLPGDIELQVASDSTNSIRASL"
     gene            complement(991..>2895)
                     /locus_tag="VIN75_22790"
     CDS             complement(991..>2895)
                     /locus_tag="VIN75_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8977061.1"
                     /translation="TQIGFTIVSLTVSLIAVLIPLLFMGDVVGRLFREFAVTLAITIL
                     ISAVVSLTLVPMMSGRMLKREDKPHGYGLKFQQGFDWIIARYDRGLVWVLDHQTTTLL
                     VALATFALTVLLYVFIPKGLFPTQDTGQLQVRVQSAETVSFERMSDLQRQAARAMLQD
                     PAVDNIASIVGVDGANNTMLNAGTMLVNIKRGASGTQEQIMDRLRDRAQAVAGVTLYL
                     QPTQDLTIDSDTGPTQYRVSLEGADDATVALWADKLVDRLRGDSKVRNVVTDVGQTGL
                     AVEVDVDRDTASRLGISAATVDDALYSAYGQRIVSTIFTETNQYRVILEARPDQARTP
                     TSIGDLRVLTTSGQATPLSAFATIREVQSPLQITHVAQYPATTVGFDTADGASLGTAV
                     DSIRAAARDIALPSSVTMTPLGAAGAYEKSLSNQLWLILAAVICVYIVLGVLYESYVH
                     PLTILSTLPSAGVGALLALLIGGQDLGVIGIIGIILLIGIVKKNAIMMIDFAIDAERG
                     EGKAPREAIHQAALLRLRPILMTTFAALFAAVPLMFGWGEGKELRHPLGLSIFGGLLV
                     SQLLTLFTTPVIYLWFDRLGRRLRERFGSNDDDDDDEPAPQRALEAPPVPQRIGFDPD
                     APHPAPGAGP"
BASE COUNT          413 a          980 c         1052 g          450 t
ORIGIN      
        1 gcagcgacgc gcggatcgag ttggtggagt ccgatgccac ctgcagctcg atgtccccgg
       61 gcaactgcgc cttgagctcc ggcacgagcg cgcgcacgcc gttgacggtc tcgatgatgt
      121 tggcgccggg ctggcgcgtg acgatgacga tcaccgccgg gctgccgttg aacaggccga
      181 gcgtgcgcac gtcctcgggg ccgtcggtga cattggcgat gtccgacagc ttgaccgccg
      241 cgccgttgcg ccatgccacg gtcaggttcc tgtattccgc ggcggtgtgc cccggctgcc
      301 gcgtgtagat ctgcagccgc tgcacgccgt cctcgatgct gcccttgggc cgtgtcgcgt
      361 tggaggcctg gatcgcggcg cgcacgtcct cggccgggat gccgaggtcg ttgagcgcca
      421 gcaggttcac gtcgatgcgc acggccggca gcgagccgcc gccgatctcg acgtcgccca
      481 cgccctgcac ctgcgccacc ttctgctgca cgatgttgga gacggcgtcg tagatctgct
      541 ggggcgtgcg cgtccgggag gtcagcgcca ggatgatgca gggcgcgtcc gacgggttgg
      601 ccttgcggta ggtcgggttg ctcttgagcg tggcgggcag gtcgactcgt gccgcgttga
      661 tcgcggcctg cacctcgcgc gcggcgccgt cgatgtcgcg gttcaggtcg aactgcaggc
      721 tcacgcgcgt ggagccggtg cccgagttgg acgtgatctc gttgacgccc gagatcgtgc
      781 cgaggcgccg ctcgagcggg gtggccacgc tgttggccat cgtgtcgggg ctggcgccgg
      841 ggatgctggc cgacaccgag atggccgggt agtcgacctg gggcagcggc gccaccggca
      901 gcacgaagaa gcccgcgatg cccgccagcg cgacgccggc ggtcagcagc accgtcgcga
      961 tcgggcgccg gacgaagggc gacgacaggt tcatggcccc gctcccggcg ccggatgcgg
     1021 cgcgtcgggg tcgaagccga tccgctgcgg caccggcggc gcctcgagcg cccgctgcgg
     1081 cgccggctcg tcgtcgtcgt catcgtcgtt gctgccgaag cgctcgcgca ggcggcggcc
     1141 gaggcgatcg aaccacaggt agatcaccgg cgtcgtgaac agcgtcagca gctggctcac
     1201 cagcaggccg ccgaagatcg acaggcccag cgggtggcgc agctccttgc cttcgcccca
     1261 gccgaacatc agcgggacgg ccgcgaacag cgcggcgaag gtcgtcatca ggatcggccg
     1321 cagccgcagc agcgccgcct ggtggatggc ctcccgcggc gccttgccct cgccgcgctc
     1381 ggcgtcgatg gcgaagtcga tcatcatgat cgcgttcttc ttgacgatgc cgatcagcag
     1441 gatgatgccg atgatgccga tcacgcccag atcctgcccg ccgatcagca gcgccagcag
     1501 cgcgccgacg ccggccgagg gcagtgtcga caggatcgtg agcgggtgca cgtagctctc
     1561 gtacagcacg cccagcacga tgtagacgca gatgacggcc gccaggatca gccacagctg
     1621 gttggacagc gacttctcgt aggcgcccgc ggcgcccagc ggcgtcatcg tgacggacga
     1681 gggcagggcg atgtccctgg ccgcggcgcg gatcgaatcg accgccgtgc ccagcgacgc
     1741 gccgtcggcg gtgtcgaagc cgaccgtcgt ggccgggtac tgcgccacgt gcgtgatctg
     1801 cagcggcgac tgcacctcgc ggatcgtcgc gaacgcggac agcggcgtgg cctggccgct
     1861 ggtggtcagc acgcgcaggt cgccgatcga cgtcggcgtg cgcgcctggt cgggcctggc
     1921 ctcgaggatc acgcggtact ggttcgtctc ggtgaagatg gtcgagacga tgcgctggcc
     1981 gtaggcgctg tagagcgcgt cgtcgacggt ggcggcgctg atgcccaggc gcgacgccgt
     2041 gtcgcggtcg acgtccacct cgacggccag gccggtctgg ccgacgtcgg tgacgacgtt
     2101 gcgcaccttc gagtcgccgc gcagccggtc gaccagcttg tcggcccaca gtgccacggt
     2161 ggcgtcgtcg gcgccttcca gcgagacgcg gtactgcgtc gggcccgtgt cggagtcgat
     2221 ggtgaggtcc tgcgtgggct gcaggtacag cgtcacgccg gcgacggcct gcgcgcggtc
     2281 gcgcagccgg tccatgatct gctcctgcgt gccgctcgcg ccgcgcttga tgttcacgag
     2341 catcgtgccg gcgttgagca tcgtgttgtt ggcgccgtcc acgcccacga tcgatgcgat
     2401 gttgtcgacc gccggatcct gcagcatggc gcgcgcggcc tggcgttgca ggtcggacat
     2461 gcgctcgaag ctgaccgtct cggcgctctg cacgcgcacc tgcagctggc cggtgtcctg
     2521 cgtcgggaac aggcccttgg ggatgaacac gtacagcagc acggtcagcg cgaaggtggc
     2581 cagcgccacc agcagcgtgg tcgtctggtg gtccagcacc cacacgaggc cgcggtcgta
     2641 gcgcgcgatg atccagtcga agccctgctg gaacttcagg ccgtagccgt gcggcttgtc
     2701 ctcgcgcttg agcatgcgac ccgacatcat cggcaccagc gtcagcgaca ccaccgccga
     2761 gatcaggatc gtgatcgcca gcgtcaccgc gaactcgcgg aacagccggc cgaccacgtc
     2821 gcccatgaac agcagcggga tcagcaccgc gatcagcgag acggtcagcg acacgatcgt
     2881 gaagccgatc tgcgt
//
LOCUS       DATCRO010000836         5589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_234255, whole genome shotgun sequence.
ACCESSION   DATCRO010000836 DATCRO010000000
VERSION     DATCRO010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234255"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VIN75_22795"
     CDS             complement(<1..1332)
                     /locus_tag="VIN75_22795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8977062.1"
                     /translation="MSALPEFQPLDVFACPLDGIRQIEASAGTGKTWNICGLYLRLLL
                     EKRLEVQRILVVTFTNAATAELRERIRQRIVDTLAWLRAEQAGETPAAGDEFVERLVR
                     GARERHGLDSADMIARLALAQATFDEASIFTIHGFCQRALADTPFTAQMPLQMELVQN
                     DADWVAEAANDFWRRRIAADDLDAALAGHLVASKDSPSGFAALLKRHLSKPLARAAWP
                     DDLDAARTLPVDDLATTHDIARRLWLSQREAIEELLLRSLGGALSAVSYKEDSVRAAV
                     ASWDELLHGDDALAALAVDGPKLDLLGAARLAKATKSKQATPQHEFFDVAQQLLDLRA
                     KAAQSLAYGRLALLRELLDEGARAQREAKRRQRVIAFDDMLFNLHDRLMNGDPQLAGE
                     LKKRFPAALIDEFQDTDPLQFAIFRTIYTATTDDTLSAAPLFFVGDPKQAIY"
     gene            complement(1391..4738)
                     /gene="recC"
                     /locus_tag="VIN75_22800"
     CDS             complement(1391..4738)
                     /gene="recC"
                     /locus_tag="VIN75_22800"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEY8977063.1"
                     /translation="MLHLHFANRFETLADQLATRLGQPVPGRSPFDADQVIVPSAAIT
                     RRLIIELARRHGVCANVEFSYLAHWLWKQTAQLVPELPESARFDADVFGWRILAAFDD
                     GAWSSRQPRLAAWLSNADAVMRHELARRVAGLFEQYLVYRPEWLEAWFREETALPGAD
                     TSAALDQAWQAALWRRLAHETASDGRHPILALLEMLDARGIGLAGTGRLPASAHVFCL
                     PTMPPVHLELLQRLARHVQLHLYALNPCREFWFDVVDPRRLAWLAARGELQYHEEGNR
                     LLAAWGRQTQSALSLLVNAAAEGAVEDAVFTPAARRSVLGALQDAVLDMTELAPGSLV
                     DVAADRSVEVHVCHSLTRELEVLHDRLLGLMAGDDPPQPGDILVVTPDLDAAAPLIDA
                     VFGTAPRERHLPYAISGRARAQVNAPARALLELLALVASRLPVNDVFGLLQQPVVARR
                     FGLAEDDLDQVREWLVDAGIHWALDAEHRGSFDVPAHERHSFADGLDRLFLGYALPSH
                     LASPFQGRLPAGDAEGSGALTLGAFWRFVDTLKSLRERVAAPRAPSDWPAILGDALRD
                     FVAPADADLEDLREVDEEIAKLGDQWRRSGLATPLPFDVVRAALEEALADPARGGVPT
                     GMITFAAMSSLRNVPFRVVCAIGLDDGAFPTTARPAEFDLMNLAPRAGDRQRRLDERN
                     VFLDLILSARDRVHLSHAGRSVRDNSVRPASVLVAELLETLVPALARAPGDAASRAAA
                     RAALVVEHPLQPFALQAFDVATDEPRLRSYQADYAQALRQSLAAAPTAAGNAGFDDEG
                     EAGEDDATAAAAAPFFAGALPPVDAAWRDVPLARLSAFLRNPCRFLLEQRLGIALRRD
                     DEALQDDEPFVPDNGGRAPLVQLLLPALLAGMAPEAARTLALAGTALPAGGFGLRFVE
                     RELNGLRDFAEQVREHTAQPALPPHVAELVVEIDGEPWRLHGEFADLRPRGLLRHRYA
                     KQSPIDYLEAWLPHLLLCHDAPAGVLPVTTGIARDGRFFLTECDAPKTTLQTLLRLYA
                     KGLREPLAFFPRSAWAWVEGDRDGPSKAAAVFRPGGFNEFAEGEDAGIRLALRGRPDP
                     FSPEAVDEFYANARAVFDPLRACLEVE"
     gene            complement(4860..>5589)
                     /locus_tag="VIN75_22805"
     CDS             complement(4860..>5589)
                     /locus_tag="VIN75_22805"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY8977064.1"
                     /translation="AAAAFTAFALPMPAQAITINIGGDSSTVKGSGKEVAVARQVAAF
                     SALRLDSSVDVQAHQGTTASVTVHADDNIEPLIETAVDGDTLVVRTKKGTGYYSHHKV
                     VVDVVFTSLTAVQQRGSGDLHIDKISGPKFESSISGSGDLQIDSAQVGAFALSIAGSG
                     DVVISGTADEARFGIAGSGDINARNFAARKVNVSISGSGDAHVNATEALEAKVAGSGD
                     VTYSGHPRDVSRRVSGSGSVESAN"
BASE COUNT          745 a         1935 c         2161 g          748 t
ORIGIN      
        1 gtagatcgcc tgcttgggat cgccgacgaa gaacagcggc gccgccgaca gcgtgtcgtc
       61 cgtggtggcc gtgtagatcg tgcggaagat ggcgaactgc agcgggtcgg tgtcctggaa
      121 ctcgtcgatc agcgccgccg gaaagcgctt cttgagctcg cccgcgagct gcgggtcgcc
      181 gttcatcagc cggtcgtgca ggttgaacag catgtcgtcg aaggcgatca cgcgctggcg
      241 gcgcttggcc tcgcgctggg cccgcgcgcc ttcgtccagc agctcgcgca gcaaggcgag
      301 ccggccgtag gccagcgact gcgccgcctt ggcgcgcagg tcgagcagct gctgcgcgac
      361 gtcgaagaac tcgtgctgcg gcgtcgcctg cttgctcttg gtggccttgg cgagccgggc
      421 ggcgcccagc aggtcgagct tgggcccgtc gacggccagc gccgccagcg cgtcgtcgcc
      481 gtgcagcagc tcgtcccagc tggccacggc cgcgcgcacg ctgtcttcct tgtagctgac
      541 cgcgctgagc gcgccgccca gcgagcgcag caacagctcc tcgatggcct cgcgttgcga
      601 cagccacagg cggcgggcga tgtcgtgggt ggtggccaga tcgtcgacgg gcagggtgcg
      661 cgcggcgtcc aggtcgtcgg gccaggcggc gcgcgccagt ggcttggaca ggtggcgctt
      721 gagcagcgcg gcgaagccgg agggcgaatc cttgctcgcc accaggtgcc cggccagtgc
      781 ggcgtcgagg tcgtcggccg cgatgcggcg gcgccagaag tcgttggccg cctcggccac
      841 ccagtcggcg tcgttctgca ccagctccat ctgcagcggc atctgcgcgg tgaacggcgt
      901 gtcggccagc gcgcgctggc agaagccgtg gatcgtgaag atggaggcct cgtcgaaggt
      961 ggcctgcgcg agggccaggc gcgcgatcat gtcggccgag tccaggccgt gccgctcgcg
     1021 ggcgccgcgc acgaggcgct cgacgaactc gtcgccggcg gcgggcgtct cgccggcctg
     1081 ctcggcgcgc agccaggcga gcgtgtcgac gatgcgctgg cggatgcgct cgcgcaattc
     1141 ggcggtggcc gcgttggtga aggtgacgac gaggatgcgc tgcacctcca ggcgcttctc
     1201 cagcagcagc cgcaggtaca gcccgcagat gttccacgtc ttgcccgtgc cggccgacgc
     1261 ttcgatctgg cggatgccgt ccagcgggca ggcgaagacg tccagcggct ggaattccgg
     1321 cagggcgctc atcgcgcagc ctccccccgc agcgccggtg cccatgcatc gaatcgttgt
     1381 cgccgcatgc tcattcgacc tccaggcagg cgcgcagcgg gtcgaacacg gccctggcgt
     1441 tggcatagaa ctcgtcgacg gcctcgggcg agaacgggtc cggacggccg cgcagcgcga
     1501 ggcggatgcc ggcgtcctcg ccctcggcga actcgttgaa gccgccgggc cggaagacgg
     1561 cggcggcctt ggacgggccg tcgcggtcgc cctcgaccca ggcccaggcc gagcgcggga
     1621 agaaggccag gggctcgcgc aggcccttgg cgtagaggcg cagcagcgtc tggagcgtgg
     1681 tcttcggcgc gtcgcattcg gtgaggaaga agcggccgtc gcgtgcgatg ccggtggtga
     1741 cgggcagcac gccggccggc gcgtcatggc acagcagcag gtgcggcagc caggcctcga
     1801 ggtagtcgat gggcgattgc ttcgcatagc gatggcgcag caggccgcgc gggcgcaggt
     1861 cggcgaactc cccgtgcagg cgccagggct cgccgtcgat ctcgacgacg agttcggcca
     1921 cgtgcggcgg cagcgccggc tgggcggtgt gctcgcgcac ctgctcggcg aagtcgcgca
     1981 ggccgttcag ctcgcgctcc acgaagcgca ggccgaagcc gccggcgggt agcgcggtgc
     2041 cggccagcgc gagggtgcgt gcggcctcgg gcgccatgcc cgcgagcagc gcaggcagca
     2101 gcagttgcac cagcggcgcg cggccgccgt tgtcaggcac gaagggctcg tcgtcctgca
     2161 gcgcttcgtc gtcgcgtcgc agtgcgatgc ccaggcgctg ttcgagcagg aagcggcacg
     2221 ggttgcgcag gaaggcggac aggcgcgcga gcggcacgtc gcgccaggcc gcgtcgaccg
     2281 ggggcagcgc accggcgaag aagggcgcgg ccgcggcggc cgtcgcgtcg tcttcgccgg
     2341 cctcgccctc gtcgtcgaag ccggcattgc ctgcggccgt cggtgcggcg gccaggctct
     2401 ggcgcagcgc ctgcgcatag tcggcctggt agctgcgcag gcgcggttcg tcggtggcga
     2461 cgtcgaaggc ctgcagcgcg aacggctgca gcgggtgctc gaccaccagc gccgcacgcg
     2521 cggccgcgcg tgacgcggcg tccccgggtg cgcgcgcgag cgccggcacg agcgtctcca
     2581 gcaactcggc caccagcacc gaggccgggc gcaccgagtt gtcgcgcacg ctgcggcccg
     2641 cgtggctcag gtgcacgcgg tcgcgcgcgg acaggatcag gtcgaggaac acgttgcgct
     2701 cgtcgagccg gcgctggcgg tcgcccgcgc gcggcgcgag gttcatcagg tcgaattcgg
     2761 ccggccgcgc cgtggtgggg aacgcgccgt cgtccaggcc gatggcgcac accacgcgga
     2821 acggcacgtt gcgcaggctg ctcatcgccg cgaacgtgat catgccggtg ggcacgccgc
     2881 cgcgcgcggg gtcggccaac gcctcctcca gcgccgcgcg cacgacgtcg aacggcagcg
     2941 gcgtggccag cccgctgcgg cgccactggt cgccgagctt cgcgatctcc tcgtcgacct
     3001 cccgcaggtc ttcgagatcg gcgtcggctg gcgccacgaa gtcgcgcagc gcgtcgccga
     3061 ggatcgcggg ccagtcggac ggcgcgcgcg gcgcggccac gcgctcgcgc agcgacttga
     3121 gcgtgtcgac gaaacgccag aacgcgccca gcgtgagcgc gccggagcct tcggcgtcgc
     3181 cggcgggcag gcggccctgg aacggcgagg ccaggtgcga gggcagcgcg tagcccagga
     3241 acaggcggtc caggccgtcg gcgaagctgt ggcgctcgtg cgcgggcacg tcgaagctgc
     3301 cgcgatgctc ggcgtcgagc gcccagtgga tgccggcgtc gacgagccac tcgcgcacct
     3361 ggtcgaggtc gtcctcggcc aggccgaagc ggcgtgcgac caccggttgc tgcagcaggc
     3421 cgaacacgtc gttgacgggc aggcgcgatg cgaccagcgc cagcagctcg agcagcgcgc
     3481 gcgccggcgc attgacctgg gcgcgggcgc ggccgctgat ggcgtagggc aggtgccgct
     3541 cgcgcggcgc ggtgccgaag accgcgtcga tcagcggcgc ggcggcgtcg aggtcgggcg
     3601 tgacgacgag gatgtcgccc ggctgcggcg ggtcgtcgcc ggccatcagt ccgagcaggc
     3661 ggtcgtgcag cacctcgagc tcgcgcgtga gcgagtggca cacgtgcacc tcgacgctgc
     3721 ggtcggccgc gacatcgacc agcgagccgg gcgcgagctc ggtcatgtcg aggacggcgt
     3781 cctgcagcgc gcccagcacg ctgcggcgcg cggccggcgt gaacaccgcg tcctcgaccg
     3841 cgccctcggc cgcggcgttc accagcaggc tcagcgccga ctgcgtctgc cggccccagg
     3901 cggccagcag tcggttgcct tcctcgtggt attgcagctc gccgcgcgcc gccagccagg
     3961 ccagccggcg cgggtcgacg acgtcgaacc agaactcccg gcaggggttc agcgcgtaca
     4021 ggtgcagctg gacgtggcgg gccaggcgct gcagcagctc caggtgcacg gggggcatcg
     4081 tcggcaggca gaacacgtgc gcgctggcgg gcagccggcc cgtgccggcc aggccgatgc
     4141 cgcgcgcgtc cagcatttcg agcagcgcga ggatggggtg gcggccgtcg ctggccgtct
     4201 cgtgcgcgag ccggcgccac agggcggcct gccaggcctg gtccagcgcg gcgctcgtgt
     4261 ccgcgccggg cagggcggtc tcctcgcgga accaggcttc cagccactcg ggccggtaga
     4321 cgagatactg ctcgaacagc cccgcgacgc ggcgcgcgag ctcgtggcgc atcacggcgt
     4381 cggcgttgga cagccaggcc gcgagccggg gctgccggct gctccatgcg ccgtcgtcga
     4441 aggcggcgag gatgcgccag ccgaacacgt cggcatcgaa gcgggccgac tccggcagct
     4501 cgggcaccag ctgcgccgtc tgcttccaga gccagtgcgc gaggtaggag aactcgacgt
     4561 tggcgcagac gccgtggcgg cgcgccagct cgatgatcag ccgccgcgtg atggccgcgc
     4621 tcggcacgat gacctggtcg gcgtcgaacg gcgagcggcc cggcaccggc tggcccagtc
     4681 gcgtcgcgag ctggtcggcg agggtctcga agcggttggc gaagtggaga tggagcatgc
     4741 cgggcaggga ggtcgatcac gccgattgtg acggctgcgc gggcacccgc ccgcggcttc
     4801 ccgaccactt atcctgcccg ttggcgggct caggggatga gcccggcggg aaaggccgct
     4861 cagttggccg attcgaccga gcccgagccc gacacgcggc gcgagacgtc gcgcgggtgc
     4921 cccgagtagg tgacgtcgcc cgagccggcg accttggcct cgagggcctc ggtggcgttg
     4981 acgtgcgcgt cgccggaacc gctgatgctg acgttgacct tcctggcggc gaagttgcgc
     5041 gcgttgatgt cgcccgatcc ggcgatgccg aagcgcgcct cgtcggccgt gcccgagatg
     5101 acgacgtcgc ccgagcccgc gatgctcagc gcgaaggcgc cgacctgcgc gctgtcgatc
     5161 tgcaggtcgc ccgagccgga gatcgacgac tcgaacttcg ggccgctgat cttgtcgatg
     5221 tgcaggtcgc cgctgccgcg ctgctgcacg gcggtcagcg acgtgaagac gacgtcgacc
     5281 acgaccttgt ggtggctgta gtagccggtg cccttcttcg tgcgcacgac cagcgtgtcg
     5341 ccgtcgacgg cggtctcgat cagcggctcg atgttgtcgt cggcgtggac ggtgaccgag
     5401 gccgtcgtgc cctggtgggc ctggacgtcg accgagctgt ccaggcgcag cgccgagaac
     5461 gcggccacct gacgcgccac ggcgacttcc ttgcccgaac ccttgaccgt gctcgagtcc
     5521 ccgccgatgt tgatcgtgat ggcctgggcc ggcatcggca gcgcgaaggc ggtgaacgcg
     5581 gccgcggcg
//
LOCUS       DATCRO010000837         6759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_976725, whole genome shotgun sequence.
ACCESSION   DATCRO010000837 DATCRO010000000
VERSION     DATCRO010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976725"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="VIN75_22810"
     CDS             <1..2068
                     /locus_tag="VIN75_22810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8977065.1"
                     /translation="HTDPGDELKPGCLIYLPVYRKGASVNSVAERRASAIGWVRAAFR
                     VGDLMSTLYGEQSPGLVVRVHDGADTGAASLMYRSDSDGGTASDALIAHARFQAQEYV
                     TLAGHTWTVQLSSLPEFERVHERNASTVILVAGALLSLLLAVITHQLVTGRTRAYAAA
                     HRMTRELRASEERYRRIVETASEGIWLLDEFHRLAFVNPRIAQWLGYGEDVMRGRPLD
                     DFMDPEEAERCRAALAAQLADDGGPVELRLRRADGSPMWVSLSTRPIRDDVGNPTGAL
                     GMLTDINERRQAEERRAALETQLRDAQKMEAIGTLAGGIAHDFNNILAAIIGNVAAAQ
                     HDAAVGLSCDPSLLQIERAALRARSLVQQILTFSRMQVQELHTQALQPVIDETLDMLR
                     AQVPSHVELDVAMPAEPVHVRADATQIQQIVMNLCTNAWHALPAGRGRIEVGLAAESR
                     IEPAQADAAGSWPEALRQGRRAHLWVADDGCGMDEATKARVFEPFFTTKQVGRGTGLG
                     LAVVHGIVSVHGGAIHVDSSPGIGSRFDLWFPLQDAPEDAVDSQFSGLVAPPGNGQHV
                     LCVDDDPAMVLMVDGLLRRSGYEVTAYEDPAAALARVQADPRAFDILVTDYNMPEMNG
                     MQLATEVSRAAPALPIIITSGFISDEMRHSAAELHVGALLQKEYTLERLAGLVHAVLD
                     QHRRAG"
     gene            2472..4391
                     /locus_tag="VIN75_22815"
     CDS             2472..4391
                     /locus_tag="VIN75_22815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8977066.1"
                     /translation="MTEPVAAPCGSWLSPITAARVAAGVRPVSAPRIVGGRVLWLQGL
                     PEEGGRLAVATAGTDGARRIVTPAPFNVRSRVHEYGGGAFVAAGDTIWFSNHADNLVY
                     AQVGDAAPVALTSDGRQRHADFEIDARRKRLIAVREDHGVAGEPRNSLVALDLAGGGS
                     TTLAEGADFYASARVSPEGRRVAWLCWNHPQMPWQGTELWLAAFADDGSLIHASRRAG
                     GPDESLCQPVWAPDGKLYVVSDRSGFWNLYRLEVDGLVPVCPMAAEFGMPQWVLAQSS
                     YGFAGPDDIVAVCRENAVSRLLRIDARTGAAATIPTPFEDVVELRVAGGAAVVEAGTP
                     TMPACIASVSLADGAVTVIARSAEELPDAGSLSVPRAISFPSANGRTAHAFHYPPRNA
                     GYRVPDGERPPLIVIGHGGPTGMAGNTLKLTTQFWTSRGFAVLDVNYGGSTGFGRRYR
                     ELLSGQWGVVDVEDCVAGARWLVDQGLADAARLAIRGGSAGGYTALAALAFHDVFKAG
                     ASYYGVGDLRALDAETHKFESRYNNDLLAPFPERERLYDERSPIHAADRLSCPVIFFQ
                     GLDDKVVLPAQAERMVAAMRAQHIPVAYLAFEGEGHGFRRKETVQRTLEAELSFYGQV
                     FGFEPADDVERVDLS"
     gene            4749..5153
                     /locus_tag="VIN75_22820"
     CDS             4749..5153
                     /locus_tag="VIN75_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8977067.1"
                     /translation="MPDPSLFSVVAGSPEPIYRQLVAQVRRCVAGGQLKAGDELPSVR
                     DLAQSLAVNPMTVSKAFGLLEAEGLVTRRRGLAMAIAEQHGRAQPMAERIDLLRPTLE
                     RAAGEARQLQLPASQVLALFKTILNEQEGLLR"
     gene            5150..6031
                     /locus_tag="VIN75_22825"
     CDS             5150..6031
                     /locus_tag="VIN75_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8977068.1"
                     /translation="MNTARNDATAGCVSLQGLSVAFGAQRVLDGLDWTLPAGQVVGLL
                     GRNGAGKTTLIETLLGLREPDAGLARVFGQPSQALPDEVRARIGYVPQRADLFEWFTA
                     DQLLAYFRSFYPRWNEAKVQGLMSRWDITRDKPIRQLSGGQQQRLSIVRALAHEPELL
                     VLDEPVASLDPAARRDFLGELVDQVADRGTTILFSTHILSDLERVAVDVAFLTGGRIA
                     LHAPLDELLESSVRLGGVPADIERFVHEHRLQALTRDARGPVIARGRDASAIPPATPT
                     VSVDPATLEDLFLALTA"
     gene            6043..>6759
                     /locus_tag="VIN75_22830"
     CDS             6043..>6759
                     /locus_tag="VIN75_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977069.1"
                     /translation="MHRADSSSLAHILASTWQQRRNAGGGLRGAAIVVALCFAAPVGL
                     SAWSLFCDPASVERVRQGILASTLVGAGALVVAAWAMLVGNVLQQNHPTMARLVPGHV
                     VRLRAALVAAWALLVLAAAAGPGFAFGAPLVWACETGLALAVLTAALRWPVLWAIGLV
                     APFVAPRLLATHEADQLVEALHATWLREDWLLTAVVLTAGALVMVGVVRSGGSRHRAG
                     YAMSRALARSFGRCAGDAAGT"
BASE COUNT          902 a         2403 c         2456 g          998 t
ORIGIN      
        1 gcacacggac ccgggcgacg agctcaagcc gggctgcctg atctacctgc cggtctatcg
       61 caagggcgcc tcggtcaact cggtggccga gcggcgcgcg agcgcgatcg gctgggtgcg
      121 tgcggcgttc cgcgtcggcg acctcatgtc cacgctctat ggcgagcaga gtccggggct
      181 ggtcgtgcgc gtgcacgacg gcgccgacac gggcgccgcc tcgctgatgt accgctccga
      241 cagcgatggc ggcaccgcca gcgacgccct gatcgcgcac gcccgcttcc aggcgcagga
      301 gtacgtcacg ctggcggggc acacctggac ggtgcagctc agctcgctgc ccgagttcga
      361 gcgcgtgcac gagcgcaacg cgtccaccgt catcctggtg gccggcgcgt tgctgagcct
      421 gctgctggcc gtcatcacgc accagctggt gaccggccgc acgcgcgcat acgcggccgc
      481 gcatcgcatg acgcgcgagc tgcgcgccag cgaggagcgt taccgccgca tcgtcgagac
      541 cgcgagcgag ggcatctggc tgctcgacga gttccaccgc ctcgcgttcg tcaacccgcg
      601 catcgcgcag tggctgggct acggcgaaga cgtcatgcgc ggccgcccgc tggacgactt
      661 catggacccc gaggaggccg agcgctgccg cgccgcgctg gccgcgcagc tcgcggacga
      721 cggcggcccg gtggagctgc gcctgcggcg ggccgacggt tcgccgatgt gggtgtcgct
      781 gtcgacgcgg ccgatccgcg acgacgtcgg caacccgacg ggcgcgctgg gcatgctcac
      841 cgacatcaac gagcgccgcc aggccgagga gcgccgcgcc gcgctcgaga cgcagctgcg
      901 cgacgcgcag aagatggagg cgatcggcac gctggcgggc ggcatcgcgc acgacttcaa
      961 caacatcctg gcggccatca tcggcaacgt ggcggcagcg cagcatgacg cggccgtggg
     1021 cctgtcgtgc gatcccagcc tgctgcagat cgagcgcgcg gcgttgcgcg cgcgcagcct
     1081 ggtgcagcag atcctcacgt tcagccgcat gcaggtgcag gagctgcaca cgcaggcgct
     1141 gcagccggtc atcgacgaga cgctggacat gctgcgcgcg caggtgccgt cgcacgtcga
     1201 gctggacgtg gcgatgccgg ccgagcccgt gcacgtgcgc gcggacgcca cgcagatcca
     1261 gcagatcgtg atgaacctgt gcaccaatgc ctggcacgcg ctgccggccg gtcgcggacg
     1321 catcgaggtg ggcctggcgg ccgagagccg catcgagccc gcgcaggccg acgcggccgg
     1381 cagctggccc gaggcgctgc gccagggccg gcgcgcgcac ctgtgggttg ccgacgacgg
     1441 ctgcggcatg gacgaggcca cgaaggcccg cgtcttcgag ccgttcttca ccaccaagca
     1501 ggtcgggcgc ggcacgggcc tcgggctggc ggtggtgcac ggcatcgtca gcgtgcacgg
     1561 cggcgcgatc cacgtggaca gctcgcccgg catcggcagc cgcttcgacc tgtggttccc
     1621 gctgcaggac gcgcccgagg acgccgtcga ttcgcagttc agcggcctcg tggcgccgcc
     1681 gggcaacgga cagcacgtgc tgtgcgtcga cgacgacccg gcgatggtgc tgatggtcga
     1741 cggcctgctg cggcgcagcg gctacgaggt gacggcctac gaggaccccg ccgcggcgct
     1801 cgcgcgcgtg caggccgacc cgcgcgcgtt cgacatcctc gtcaccgact acaacatgcc
     1861 cgagatgaac ggcatgcagc tggccaccga ggtctcgcgc gcggcgcccg cgctgcccat
     1921 catcatcacg tcgggcttca tctccgacga gatgcgccac agcgccgccg agctgcacgt
     1981 gggcgcgctg ctgcagaagg aatacacgct ggagcgcctg gccgggctgg tgcatgcggt
     2041 gctggaccag catcgccggg cgggttgaag tcttcgacgg tgccaggtct tgcctggcga
     2101 ctcgaatgcg accgacgaag gtgcccgacg aaggtgccag gtcttgccta gcaactcgaa
     2161 tgcgacgggc gaggtggcgc tttatcgacg gtgccaggtc ttgcctggca actcgaatgc
     2221 gacgggcggg gtggcgcttg gcgcggagcc ttgcccggca gggcccggca gctgtgccgt
     2281 ccctctttgc ctggcaagac ctggcacctt catgtgccgt ccccctttgc ctggcaagac
     2341 ctggcagctt cgttatttgc ctggcaagac ctggcacctt cgttgtggca ccttcgttcg
     2401 tggcaccttc gttcggcacc ttcatcccgg cgcgaggcac actgtgcgcc tcgccttcga
     2461 atcgacacgc catgaccgaa cccgtcgccg ccccctgtgg ctcctggctc tcgcccatca
     2521 ccgccgcgcg cgtcgccgcc ggcgtccggc ccgtctcggc gccgcgcatc gtcggcggcc
     2581 gcgtgctgtg gctgcagggg ctgcccgagg agggcgggcg cctggccgtc gccaccgccg
     2641 ggaccgacgg cgcgcgacgc atcgtcacac ctgcgccgtt caacgtgcgt tcccgcgtgc
     2701 acgaatacgg cggcggcgcc ttcgtggcgg ccggcgacac gatctggttc tcgaaccacg
     2761 ccgacaacct cgtgtacgcg caggtgggcg atgcggcgcc ggtcgcgttg acgtccgacg
     2821 gccgccagcg ccacgccgac ttcgagatcg acgcgcgccg caagcggctg atcgcggtgc
     2881 gcgaggatca cggcgtcgcg ggcgagccgc gcaactcgct ggtcgcgctg gaccttgccg
     2941 gcggcggctc gacgacgctg gcagagggcg ccgatttcta cgcctcggcg cgcgtgtcgc
     3001 ccgaggggcg gcgcgtcgca tggctctgct ggaaccatcc gcagatgccg tggcagggca
     3061 ccgagctgtg gctcgcggcc ttcgccgacg acggctcgct gatccacgcg agccgccggg
     3121 ccggcggccc ggacgagtcg ctgtgccagc cggtgtgggc gccggacggc aagctgtacg
     3181 tggtgtccga ccgcagcggc ttctggaacc tgtaccggct ggaggtcgac ggcctggtgc
     3241 cggtctgccc gatggccgcg gaattcggca tgccccagtg ggtattggcg caatcgtcct
     3301 acggcttcgc cgggcccgac gacatcgtcg ccgtctgtcg cgagaacgcc gtcagtcggc
     3361 tgctgcgcat cgacgcgcgc acgggcgccg cggcgacgat cccgacgccg ttcgaggacg
     3421 tcgtcgagct gcgcgtggcc ggcggcgcgg cggtggtcga ggccggcacg ccgacgatgc
     3481 cggcctgcat cgcatcggtg tcgctggccg acggcgctgt gacggtgatc gcgcgcagcg
     3541 ccgaggaact gcccgacgcc ggcagcctgt cggtgccgcg cgcgatcagc ttcccgagcg
     3601 ccaacggccg cacggcgcat gccttccact atccgccgcg caacgcgggg tatcgcgtgc
     3661 cggatggcga gcgtccgccg ctgatcgtca tcggccacgg cggccccacc ggcatggccg
     3721 gcaacacgct caagctcacg acgcagttct ggacgagccg cggctttgcg gtgctggacg
     3781 tcaactacgg cggcagcacg ggcttcggcc gcaggtaccg cgagctgctg agcggacagt
     3841 ggggcgtcgt cgacgtggag gactgcgtcg ccggcgcacg ctggctggtc gaccagggcc
     3901 tggccgacgc cgcgcgactg gccatccgtg gcggcagcgc cggcgggtac acggcgctgg
     3961 ccgcgctcgc gttccacgac gtgttcaagg ccggcgccag ctactacggc gtgggcgacc
     4021 tgcgcgcgct cgacgccgag acgcacaagt tcgagtcccg ctacaacaac gacctgctgg
     4081 cgccgttccc cgagcgcgaa cgcctgtatg acgagcgctc gccgatccac gccgccgacc
     4141 ggctgtcgtg cccggtgatc ttcttccagg ggctggacga caaggtcgtg ctgccggcgc
     4201 aggcggagcg catggtcgcc gcgatgcgtg cgcagcacat tccggtggcc tatctcgcgt
     4261 tcgagggcga ggggcatgga ttccggcgca aggagacggt gcagcggacg ctggaggcgg
     4321 aactctcgtt ctatggccag gtgttcgggt tcgagccggc ggacgatgtg gagcgggtcg
     4381 acctgtcctg agtccgcatc gcgtttcccg cggccgcccc gctgcggcgc tggctgcctg
     4441 cgcgcgactg attgcccgcg gcgccgggcc aaaccgcggg tcaaagagcg gggtcaaaga
     4501 gcggggtcaa agagcggggt caggtcttga atcgtgcact ttcaaaccgc ggggtcaggt
     4561 cttgaatctt gcactctcca atgtcaccgc ccagattgca cgattcaaga cctgaccccg
     4621 caacattcaa gacctgaccc cgctgcgacc ccgctgcatg accccgctgc agacccgcca
     4681 cggatccggc ttgcgtatgg tgtcctggtg tattacatta ctaatacaca aagacaggac
     4741 gccaccccgt gcccgatcct tccctcttct ccgtcgtcgc cggctccccc gagccgatct
     4801 accggcagct cgtcgcgcag gtgcggcgct gtgtcgccgg cgggcagctg aaggccggtg
     4861 acgagctgcc ttcggtgcgc gatctcgcgc agtcgctggc cgtcaatccg atgaccgtgt
     4921 ccaaggcgtt cggcctgctc gaggcggaag gcctggtcac gcgccggcgc gggctggcca
     4981 tggcgatcgc cgagcagcac gggcgcgcac agcccatggc cgagcggatc gacctgctgc
     5041 gtccgacgct cgagcgcgcc gccggcgagg cgcgccagct gcagttgccg gccagccagg
     5101 tgctggccct tttcaagacc atcctgaatg aacaagaagg gcttttgcga tgaacaccgc
     5161 tcgaaacgac gcgacggccg gatgcgtgtc gctgcaaggg ctgtccgtgg cgttcggcgc
     5221 gcagcgcgtg ctcgatggcc tggactggac gctgcccgcc ggccaggtgg tgggcctgct
     5281 gggccgcaac ggcgccggca agaccacgct catcgagacg ctgctgggcc tgcgcgagcc
     5341 cgacgccggc ctggcccgcg tgttcggcca gccgtcgcag gcgctgcccg acgaggtgcg
     5401 cgcgcgcatc ggctacgtgc cgcagcgcgc ggacctgttc gagtggttca ccgccgacca
     5461 gttgctggcc tatttccgca gcttctatcc gcgctggaat gaggccaagg tgcagggcct
     5521 gatgtcgcgc tgggacatca cccgcgacaa gccgatccgg cagctgtccg gcggccagca
     5581 gcagcggctg tccatcgtgc gcgcgctggc ccacgagccc gagctgctgg tgctggacga
     5641 gcccgtcgcc agcctcgacc cggccgcgcg ccgcgacttc ctcggcgagc tggtcgacca
     5701 ggtggccgac cgcggcacga cgatcctgtt ctccacgcac atcctgtccg acctcgagcg
     5761 cgtggccgtg gacgtggcgt tcctcaccgg cggccgcatc gcgctgcacg cgccgctgga
     5821 cgagctgctg gaatcgagcg ttcggctggg cggcgtgccg gccgacatcg agcgcttcgt
     5881 gcacgagcat cgcctgcagg cgctcacgcg tgacgcgcgc ggacccgtga tcgcgcgcgg
     5941 tcgcgacgcg tccgccattc cgccggccac gcccacggtc agcgtcgatc cggccacgct
     6001 cgaggacctg ttcctcgcgc tcaccgcgtg aaggaggcgg ccatgcatcg cgccgactcg
     6061 tcctcgctcg cgcacatcct cgcgtcgacc tggcagcagc ggcgcaacgc cggcggcggc
     6121 ctgcgcggcg cggccatcgt cgtcgcgctg tgctttgccg caccggtggg cctgtcggcc
     6181 tggagcctgt tctgcgaccc ggccagcgtc gagcgcgtgc gccagggcat cctggccagc
     6241 accctggtcg gcgccggcgc gctggtggtc gccgcctggg cgatgctggt gggcaacgtg
     6301 ctgcagcaga accatccgac gatggcgcgg ctggtgccgg gccacgtcgt gcggctgcgt
     6361 gccgcgctgg tggcggcgtg ggccctgctg gtgctggccg cggcggccgg gcccggcttc
     6421 gcgttcggag cgccgctggt gtgggcctgc gaaacgggcc tcgcgctcgc ggtgctgacg
     6481 gcggcgctgc gctggccggt gctgtgggcg atcgggcttg tcgcgccctt cgtggcgccg
     6541 cggctgctgg cgacgcacga ggccgaccag ctggtcgaag cgctgcacgc gacctggctg
     6601 cgcgaggact ggctgctgac ggccgtcgtc ctcaccgcgg gcgcgctggt gatggtgggg
     6661 gtcgtgcgca gcggcggcag ccgccaccgg gccggctacg cgatgagccg cgcgctggcg
     6721 cgcagcttcg gccggtgcgc cggggatgcg gcggggacc
//
LOCUS       DATCRO010000838         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_921143, whole genome shotgun sequence.
ACCESSION   DATCRO010000838 DATCRO010000000
VERSION     DATCRO010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921143"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..804)
                     /locus_tag="VIN75_22835"
     CDS             complement(103..804)
                     /locus_tag="VIN75_22835"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY8977070.1"
                     /translation="MPDNNDFIEVYDDALDAATCRALVERFERGGQARRGATGSGVDT
                     SMKDSWDIALDGRPDWAEARQALDDAAVRGMRRYLRRYAHVALAPLQLKMRDPATGAL
                     RLLDADGVAALNDEFLDGLIAKVFRPGGINIQKYVAGQGGYPYWHCEQYPRMDGGEAL
                     HRAVLWTIYLNDGFAAGETEFLYQQRKVRPRTGSLLIAPAAFTHTHRGNRPEGGDKFI
                     ATSWILYRRAEQLYA"
     gene            complement(839..1012)
                     /locus_tag="VIN75_22840"
     CDS             complement(839..1012)
                     /locus_tag="VIN75_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977071.1"
                     /translation="MSFLLYTIGLVLLIGGVAWALVTANVASTYIAIICLVIAGLGVM
                     MAVSRTRQKDPPN"
     gene            complement(1080..1352)
                     /locus_tag="VIN75_22845"
     CDS             complement(1080..1352)
                     /locus_tag="VIN75_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977072.1"
                     /translation="MPTFRQTLVATFAAACAIAAAPAQAKGCIKGAVVGGVAGHVAGH
                     HAVAGAVVGCAVGHHLAARKEREEREERAAARAAAPAPVPASAPRP"
     gene            1606..2376
                     /locus_tag="VIN75_22850"
     CDS             1606..2376
                     /locus_tag="VIN75_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977073.1"
                     /translation="MNALRHDPAPGRAAAPLVALAVGLGALGVLSLGSGDFAFQWQPV
                     PPDMPSRGVLAMATGGLEVAAAVLLLSGRLRAVGAWLTAVLLLGWVALHVPAVARHPA
                     SIADWLGLAETAAMASAALMFATAWAGIAAAGAVRRGAFVAFGLCALVFGLSHVKYAE
                     FTASMIPPWLPGRVALACLTGAAHALAGLAILTGVRRTLAAWLECLMMACFVVLVHAP
                     RVLAHPDSRMEWTMLFVAVALSASAGLVAAMSGRDGRR"
     gene            2493..3509
                     /locus_tag="VIN75_22855"
     CDS             2493..3509
                     /locus_tag="VIN75_22855"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977074.1"
                     /translation="MTEPLPPNAFEPAPLRRGTFHNASGREKHGLANVLRWLLTRSPG
                     PWPTHVEDVQVAPPADRVDDGSIRATIIGHATVLVQVAGLNVLTDPVMSARIGPTPWT
                     GPRRVRPPAVPFEAIPRIDVVLLSHDHYDHLDRPTLRQLAARDNPLVLTGKRVGRKVP
                     SRKVVELDWWESHALGEGLSATYVPAEHFSGRGLFDRDKSLWGGFVLETPHGKVFFAG
                     DTARGTHFRAIRERFGPMTLSLLPIGAYAPRWFMERVHMDPDEALQASLTLESQTSLA
                     IHFATFNLADDSYDAPTRALARAVSQLSGSPAGSDFRVTPFGEATIVRPGEDLDAGAR
                     RRYA"
BASE COUNT          465 a         1406 c         1379 g          544 t
ORIGIN      
        1 ggcaccgccc cgatgcatca ttggcgtgcc ggaggcgaat cgaggcggag ccagacccct
       61 ccggccggcg gagccaacct cttcgcgggg ccgagccgtt tctcaggcgt acaactgctc
      121 ggcgcgccgg tacaggatcc agctcgtcgc gatgaacttg tcgccgccct cgggccggtt
      181 gccgcggtgg gtgtgcgtga atgccgccgg ggcgatcagc aggctgccgg tgcgcgggcg
      241 gaccttgcgc tgctggtaga ggaactccgt ctcgccggcg gcgaagccgt cgttcaggta
      301 gatcgtccac agcacggcgc ggtgcagcgc ctcgccgccg tccatcctcg ggtactgctc
      361 gcaatgccag tacgggtagc cgccctggcc cgcgacgtac ttctggatgt tgatgccgcc
      421 ggggcggaac accttggcga tgaggccgtc gaggaactcg tcgttcaacg cggcgacgcc
      481 gtcggcatcg agcaggcgca gcgcgcccgt ggccggatcg cgcatcttga gctgcagcgg
      541 cgcgagcgcc acgtgcgcgt agcgccgcag gtagcggcgc atgccgcgca cggcggcgtc
      601 gtcgagggcc tggcgcgcct cggcccagtc gggacggcca tcgagcgcga tgtcccagct
      661 gtccttcatg ctcgtgtcga cgccgctgcc cgtggcgccg cgccgggcct ggccgcctcg
      721 ctcgaagcgc tcgacgagcg cgcggcacgt ggcggcgtcg agggcgtcgt cgtagacctc
      781 gatgaaatcg ttgttgtcgg gcatgggccc ggattctggg cccgtgccgc gacgcgggtc
      841 aattcggcgg atccttctgg cgggtgcgcg acaccgccat catcacgccg agccccgcga
      901 tgaccaggca gatgatcgcg atataggtcg acgcgacgtt cgcggtgacg agggcccagg
      961 cgacgccgcc gatgagcagg accaggccga tggtgtagag caggaacgac atgcggtcac
     1021 cccgtttctt gttgacatgg ccgcatacag gcacgcgtcg tgccggggtt cgcccgaggt
     1081 cacgggcgcg gcgcgctcgc cggcacgggc gcaggcgccg ccgcgcgggc ggccgcgcgc
     1141 tcctcgcgtt cctcgcgctc cttcctggcg gccaggtgat ggccgaccgc gcagccgacg
     1201 accgcgccgg ccaccgcgtg gtgcccggcg acatggccgg cgacgccgcc gacgacggcc
     1261 cccttgatgc agcccttggc ctgcgccggc gcggcggcga tggcgcaggc ggcggcgaac
     1321 gtggcgacga gcgtttggcg gaacgtgggc atggcgaagt cctttcgtgg gtgtcgacca
     1381 gagtccgggc gatggacgca ctgtcgctgc gcacgcgcca cgacgccagg gccatgcgac
     1441 gcgcggccgc cgcgcaggga ccagctgctc ccgaggcgga cagcccgcgt ggccctgcga
     1501 gcgagggatt ccacgccagg cggcggacgc gccagcgcgt acattgcgca tctgctcgac
     1561 cacccggccg cgtccgcccg acacgccgcc ctcgcaggag agacgatgaa cgcgttgcga
     1621 cacgacccgg cccccggcag ggccgccgcg cccctggtcg ccctggccgt cggcctgggt
     1681 gcgctgggcg tgctgtcgct gggcagcggc gacttcgcct tccagtggca gcccgtgccg
     1741 ccggacatgc cttctcgcgg cgtgctggcg atggccaccg gcggcctgga ggtggcggcg
     1801 gccgtgctgc tgctgtcggg ccggctgcgc gcggtcggcg cctggctgac ggccgtcctg
     1861 ctgctcggat gggtcgcgct gcacgtgccc gcggtcgccc ggcaccccgc gtcgatcgcg
     1921 gactggctgg gcctggccga gaccgccgcg atggccagcg cggcgctgat gttcgcgacg
     1981 gcgtgggccg gcatcgccgc cgccggcgcc gtccggcgcg gggcgttcgt ggccttcggc
     2041 ctgtgcgcgc tcgtgttcgg cctgtcgcac gtcaagtacg ccgagttcac ggcctcgatg
     2101 atcccgccct ggctgcccgg acgcgtggcg ctggcctgcc tcacgggcgc ggcgcacgcg
     2161 ctcgcgggcc tggccatcct caccggcgtg cgccgcacgc tcgccgcgtg gctggagtgc
     2221 ctgatgatgg cctgcttcgt cgtgctggtg cacgcaccgc gggtgctcgc gcatcctgac
     2281 agccgcatgg aatggacgat gctgttcgtc gccgtggcgc tgagcgcctc ggccggcctg
     2341 gtggcggcaa tgtccggccg cgacggccga cgctgagcag cccggcaacg cggcaggaat
     2401 tctcactcga cagcaaaact ttccgaccgc gttcacacac cgtgcgccag gacgcacgta
     2461 cagtgcgcgg gcgaacccaa cgagcctgtg ccgtgaccga gcccctgccg cccaacgcct
     2521 tcgagcccgc cccgctgcgc cgcggcacgt tccacaacgc gagcggccgc gagaagcacg
     2581 ggctcgccaa cgtcctgcgc tggctgctga cgcgctcgcc gggcccgtgg ccgacgcatg
     2641 tcgaggacgt gcaggtcgcc ccgcccgccg atcgcgtgga cgacggctcg atccgcgcga
     2701 ccatcatcgg ccacgccacc gtgctggtgc aggtggccgg cctgaacgtg ctcaccgacc
     2761 cggtgatgtc cgcgcgcatc ggcccgacgc cctggacggg tccgcggcgc gtgcggccgc
     2821 cggccgtgcc gttcgaggcc atcccgcgca tcgacgtggt gctcctgagc cacgaccact
     2881 acgaccacct cgaccgcccc acgctgcgcc agctcgccgc gcgcgacaac ccgctcgtgc
     2941 tcaccggcaa gcgcgtgggc cgcaaggtgc cgtccaggaa ggtcgtcgag ctggactggt
     3001 gggagagcca tgcgctgggc gaggggctgt cggccaccta cgtgccggcc gaacacttct
     3061 ccggccgcgg cctgttcgat cgcgacaagt cgctgtgggg cggcttcgtg ctggagacgc
     3121 cgcacggcaa ggtcttcttc gccggcgata cggcacgggg tacgcacttc cgcgccatcc
     3181 gcgagcgctt cggcccgatg acgctgtcgc tgctgccgat cggcgcctac gcgccgcgct
     3241 ggttcatgga gcgcgtgcac atggatccgg acgaggcgct gcaggccagc ctcaccctgg
     3301 agtcgcagac ctcgctggcg atccatttcg ccacgttcaa cctggccgac gattcctacg
     3361 acgcccccac ccgcgcgctg gcccgcgccg tctcgcagct gagcggctcg cccgccggca
     3421 gcgacttccg cgtcacgccg ttcggcgagg cgacgatcgt gcggcccggc gaggacctgg
     3481 acgccggcgc gcggcgccgc tacgcctgac gaagtcttcg gcagggcgga ttggccgccg
     3541 gcgtggcccg cgccatttgg ttcttgcgaa gccggaccgc tggtcgcgcc gacgaacggc
     3601 ggtcacgtct gcgactggcc tgccctaccc ggccgttccg ccgaggcgct cccaatcgag
     3661 gtactcgcgc atcgcctgcg cgtcgcgccg cgcgaccggg cgcagctcgt cgtcgtccgg
     3721 cagtgccgcg gtcgacgtct cgagcgcgcg gcccgcgccg gcccaggtgc gcgtgccgcc
     3781 ctgcagccat gcga
//
LOCUS       DATCRO010000839         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_279781, whole genome shotgun sequence.
ACCESSION   DATCRO010000839 DATCRO010000000
VERSION     DATCRO010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279781"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1197
                     /locus_tag="VIN75_22860"
     CDS             163..1197
                     /locus_tag="VIN75_22860"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8977075.1"
                     /translation="MDTVSASTTLLGDAPVAGLRRASVPTPCEPGNILRTLVGVQVIV
                     SAGAAFGSGSLAAWVGHVVVGAAVSVPATLAWLVVCCWATGLLARLPAAGRLGCAAVV
                     GAAATWAACLPLRMLGLDAGDATNVAAFVLSGTLLGAFIYHWLELRVRATLPAATEAG
                     LIELQSRVQPHFLFNTLNTAIALVRVDPNRAEEVLENLSELFRAALGALEGDSTLGEE
                     IRLARLYLDIEGMRFGKRLEVEWDVDEGLAHVRVPSLLLQPLVENAVRHGVEPNDAGG
                     RVQIRARQRNGRAWVQVVNTVGASAPPGHGIGLVSSRERLRLMHDFRSEFQAGPIDGG
                     RFRVRLAVPL"
     gene            1237..2016
                     /locus_tag="VIN75_22865"
     CDS             1237..2016
                     /locus_tag="VIN75_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8977076.1"
                     /translation="MNRLRVLIVDDEDLARLRMRSLLADCAAPAAEVAGEAGSAAEAL
                     HWLASNQADLVLLDINMPGLDGLQLAERLRATAHPPRIVFVTAHATHALKAFEIDAVD
                     YLTKPVRRARLQEALVRVAQRLDRPVPVAPAAPVTAPAEQPVLVVSDRGRVQRVPVAD
                     ILYLKAELKYVTLRTPQHAYVLDDSLSDLEQRLGDAMLRIHRNALVARTAVTKLERRA
                     VSDGEGEGEGWAVQVGATQEWLAVSRRQLTQVRELLAEDGV"
     gene            2205..2666
                     /locus_tag="VIN75_22870"
     CDS             2205..2666
                     /locus_tag="VIN75_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8977077.1"
                     /translation="MSLTHRLAALGLVLPGTAAPGARYAAVVVHDGLAWVSGQLPREG
                     DRVVVAGKLGRDVDVPAAQRGARIALLRALAALRDELGGLERVRRILRLTAYVNSTED
                     FTQHSAVADGASALIYDLFGPELGHHARTSVGVAQLPRDGCVEIDLVAALA"
     gene            2717..3544
                     /locus_tag="VIN75_22875"
     CDS             2717..3544
                     /locus_tag="VIN75_22875"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977078.1"
                     /translation="MILTSPDVAATLEAAEAWHLRVQCETWRTLGAAHAHAVGIGGGV
                     AAFTERLFGRKLNHVTGLGMDGPVDEDALARLEAAYAERGLATEIDLCPHADARVPAL
                     LARRGYAVNAFSNTYARTLDGVSGRAPSGIEILQGDAARALFVEASVDGFSLQAQQRP
                     RELLEALARIALARDDTSVFVARIDGRVAGSAGLSAMDTPRGRVGELHIASTLPAFRG
                     RGVQAALLQARLAAARDAGCALVFVGARPANTSARNVERAGFGLAFTKTTFARPCHA"
     gene            3672..>4082
                     /locus_tag="VIN75_22880"
     CDS             3672..>4082
                     /locus_tag="VIN75_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8977079.1"
                     /translation="MRLIGMLDSPYVRRVAVTLRLLGIPFEHKPLSVFSTYEQFREIN
                     PLVKVPTLVCDDGTVLVDSNLIIDYAEALSGRSLWPAALPERTRALRTAGLGIAACEK
                     VQQIVYELQLRPADKRHAPWLERVQAQVRAALAAL"
BASE COUNT          508 a         1532 c         1487 g          555 t
ORIGIN      
        1 caagacctga ccccgcgacg attcaagacc tgaccccaaa tcgctgggga gaaccctcgg
       61 attgtaggcg gcttgccgct cgcagccttc gcgagaccgc acgtccgctt gccgcgggcg
      121 cgccttgtcc ggcggccgca cgctcgccca caatgcgggt ccatggatac cgtctccgcc
      181 tccacgacct tgctcggcga cgcgccggtg gccgggctgc gccgtgcgag cgtgcccacg
      241 ccttgcgagc ccggcaacat cctgcgcacc ctcgtgggcg tgcaggtgat cgtgtcggcc
      301 ggggccgcgt tcggcagcgg gtcgctcgcg gcctgggtcg ggcacgtcgt ggtgggcgcg
      361 gccgtgtcgg tgccggccac gctggcgtgg ctggtcgtct gctgctgggc gaccggcctg
      421 ctggcgcgcc tgccggccgc agggcgcctg ggctgcgcgg cggtggtcgg cgcggccgcc
      481 acctgggcgg cgtgcctgcc gctgcgcatg ctcgggctgg acgccggcga cgccaccaac
      541 gtcgcggcgt tcgtcctgtc cggcaccctg ctgggcgctt tcatctacca ctggctcgag
      601 ctgcgcgtgc gggccacgct gccggcggcc accgaggccg gcctgatcga gctgcagtcg
      661 cgcgtgcagc cgcacttcct gttcaacacg ctcaacaccg ccatcgcgct cgtgcgcgtc
      721 gacccgaacc gggccgagga ggtgctggag aacctgtccg agctgttccg cgccgcgctg
      781 ggcgcgctcg agggcgattc gaccctgggc gaggagatcc gcctcgcgcg cctgtacctg
      841 gacatcgagg gcatgcgctt cggcaagcgg ctggaggtcg agtgggacgt cgacgagggc
      901 ctcgcgcacg tgcgcgtgcc gtcgctgctg ctgcagccgc tggtggagaa cgcggtgcgc
      961 cacggcgtcg aacccaacga cgcgggcggg cgcgtgcaga tccgcgcgcg ccagcgcaac
     1021 gggcgggcgt gggtgcaggt ggtcaacacg gtgggcgcga gcgcgccgcc gggacacggc
     1081 atcgggctgg tgagctcgcg cgagcggctg cgcctgatgc atgatttccg ctccgagttc
     1141 caggccggcc cgatcgacgg cggacgcttc cgcgttcgcc tggccgtgcc gctgtgagac
     1201 gatgcaaccg aacgggatga cagggagcga gcggccatga accgcctgag agtgctgatc
     1261 gtcgacgacg aggacctggc ccggctgcgc atgcgcagcc tgctggccga ctgcgcggcc
     1321 ccggccgccg aggtggccgg agaggccggc agcgccgccg aggcgctgca ctggctcgcg
     1381 tccaaccagg cggacctggt gctgctggac atcaacatgc ccggcctcga cggcctgcag
     1441 ctggccgagc gcctgcgcgc cacggcccat ccgcccagga tcgtgttcgt cacggcccac
     1501 gccacccacg cgctcaaggc cttcgagatc gacgccgtcg actacctcac caagcccgtg
     1561 cgccgcgccc gcctgcagga ggcgctggtg cgcgtcgcgc agcgcctgga tcgcccggtg
     1621 cccgtcgcac ccgccgcgcc cgtgaccgcg ccggccgaac agcccgtgct ggtcgtcagc
     1681 gaccgcggcc gcgtccagcg cgtgccggtg gccgacatcc tgtacctgaa ggccgagctc
     1741 aagtacgtca cgctgcgcac gccgcaacat gcgtacgtgc tggacgactc gctgtccgac
     1801 ctcgagcagc gcctgggcga cgccatgctg cgcatccacc gcaacgcgct ggtcgcgcgc
     1861 accgccgtca ccaagctgga gcgccgggcc gtgagcgacg gcgagggcga gggcgaaggc
     1921 tgggccgtgc aggtcggggc gacgcaggag tggctggcgg tgtcgcgcag gcagttgacg
     1981 caggtgcggg agctgctcgc cgaagacggc gtgtaaacgc ttcgtcgggc ggcttacgcc
     2041 tgcggccagc ccgccctgcg acggatggcg ctccgcgaag ggccgatggg tacagcggcc
     2101 ggccctccgg gctgacacag tgcatcgccg cgtcgctccg cgtgtacctg tcgcgccacc
     2161 gccgagtacg ccagactgtg gctctcccga cgatcgccac cgccatgtcc ctcacacacc
     2221 gcctcgccgc cctcggcctc gtccttcccg gcaccgccgc ccccggcgcc cgctacgccg
     2281 cggtcgtcgt ccacgacggc ctcgcctggg tcagcggcca gctgccgcgc gagggcgatc
     2341 gcgtggtcgt cgccggcaag ctcgggcgcg acgtggacgt ccccgccgcg cagcgcgggg
     2401 cgcgcatcgc gctgctgcgc gcgctcgcgg cgctgcgcga cgagcttggc ggcctcgagc
     2461 gcgtgaggcg catcctgcgg ctgacggcct acgtcaacag caccgaggac ttcacgcagc
     2521 acagcgccgt ggccgacggc gcgtcggcgc tgatctacga cctgttcggc cccgagctgg
     2581 gccaccacgc gcgcaccagc gtcggggtcg cgcagctgcc gcgcgacggc tgcgtggaga
     2641 tcgacctcgt cgcggcgctg gcttgatcgc cggcgcgcta gactgcgccg cttcccgaca
     2701 cgccgccccc gtcaccgtga tcctcacctc gcccgacgtc gccgccaccc tcgaagccgc
     2761 ggaagcctgg cacctgcgcg tgcagtgcga gacctggcgc acgctcggcg ccgcccacgc
     2821 ccatgccgtc gggataggcg gcggcgtggc cgccttcacc gagcggctgt tcgggcgcaa
     2881 gctcaaccac gtgacggggc tcggcatgga cggccccgtc gacgaggacg cgctggcgcg
     2941 cctggaggcg gcctacgccg agcgcggtct ggccacggag atcgacctgt gcccgcacgc
     3001 ggacgcccgc gtgccggccc tgctcgcgcg ccgcggctac gcggtcaacg cattcagcaa
     3061 cacctacgcg cgaacgctgg acggggtgtc cggtcgggcg ccatccggca tcgagatcct
     3121 gcagggcgat gccgcccgcg cgctgttcgt cgaggcgagc gtcgacggct tcagcctgca
     3181 ggcgcagcag cggccgcgcg agctgctcga ggcgctcgcg cgcatcgccc tcgcgcgcga
     3241 cgacacctcg gtgttcgtgg cccggatcga cgggcgcgtc gcgggctcgg ccggcctgag
     3301 cgccatggac accccgcgcg ggcgcgtcgg cgagctccac atcgccagca cgctgcccgc
     3361 cttccgtggc cgcggcgtgc aggccgcgct gctgcaggcc cgcctcgccg cggccaggga
     3421 cgccggctgc gcgctggtgt tcgtcggcgc gcggccggcc aacacgagcg cccgcaacgt
     3481 cgagcgcgcc ggcttcgggc tcgcgttcac gaagacgacg ttcgcgcgtc cgtgccacgc
     3541 ctgacgccgc gcatgccgcg caggcatcgc ggcgatgcat gctttgcatc gcgcgggcgt
     3601 gtcgcggcgc acacgccggg ctatcgtgga gggccgccgg cgcgtgccgg cttcatcccg
     3661 aggaaacgac gatgcgcctg atcggcatgc tcgactcccc ttacgtccgc cgcgtggccg
     3721 tcacgttgcg cctgttgggg atcccgttcg aacacaagcc gctgtcggtg ttcagcacct
     3781 acgagcagtt ccgcgagatc aacccgctgg tcaaggtgcc gacgctggtg tgcgacgacg
     3841 gcaccgtgct ggtggactcg aacctgatca tcgactacgc cgaggcgctg tccggccgca
     3901 gcctgtggcc cgcggccctc ccggaacgca cgcgggcgct gcgcacggcg ggcctgggga
     3961 tcgcggcctg cgagaaggtg cagcagatcg tctacgagct gcagctgcgt cccgcggaca
     4021 agcgccatgc gccgtggctg gagcgcgtcc aggcgcaggt gcgggcggcg ctggccgcgc
     4081 tc
//
LOCUS       DATCRO010000840         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_538650, whole genome shotgun sequence.
ACCESSION   DATCRO010000840 DATCRO010000000
VERSION     DATCRO010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538650"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            804..>2009
                     /locus_tag="VIN75_22885"
     CDS             804..>2009
                     /locus_tag="VIN75_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8977080.1"
                     /translation="MSRASGASVERAAARRACAFVFIANLPHLAEAYGQDFASAASLE
                     VQRRLCAGFTSLPSSDMARLRDDCFLVWANEAFECGPFGIHEAGKASGASRRASHPVE
                     TLLAVLGAEPVRSGGIAALVQLHADWIDVRSPQQMGASEIELTLWTAQPFPDSRESRA
                     DGWRQLYRADMDVAVRVSEALRADELSFSWQPVVHPGSVATTLYRSGRARIAPSRGDA
                     ASLAPEVFMPCLQRLGLTRAFDRTAMRRAVAALRLQPSAHIGVSISAQSIQIDHWWAS
                     LLAVLEREPALASRLVIEIAGSASLPDLEAAHDFCVQLQMRGCRIAVRDFGGGADNVA
                     AVQACKPDIVKLDAALLRRARDSEFGRECLRGMLATSAHFAPHVVVDGVERDDDVHIA
                     ADAGAQWLQG"
BASE COUNT          303 a          728 c          668 g          310 t
ORIGIN      
        1 ccctcggtac ggtgccactc ccgccatggt ccgcggggag gccgcggcga tggcgaagag
       61 tgctgagaaa tcggtgcccg gcattctgca tgcacgaaca aggcagggcg ccgcgaagcc
      121 gctcgggccg ggccgccaac ccgccttcga cccggaaacc gcgatttgtc ctggtcagtg
      181 ttgacaaatg tgaactggca cacattcgcg gtccgaaagc gaatgcgggg tcctactgca
      241 atccgtagta ttcattttgt ttcacaactg tcctacggag cttgcacgtg atggcctgca
      301 gaccctcgtt ccaccgaacg tacgagccgc tcgatgcggt tcatggggcc gccgcacgaa
      361 cgcgtggccc cgccgagccg aaggccacat ccatgccgta cacctagccg ccgcgtaccg
      421 cgcgacgccg ttggcgaagc gccccctttc ccacctgtga cccgatagcc gatcgccggc
      481 actccgccag gagcgccgta ctgcgtgagg gattcgcacg tccggtgatt tcctgctttc
      541 cgtcttgatt ccgcaccacc gcgcggaatg gagaagtgtt gccgcgagaa acgcggcccc
      601 ttctcccttc accgggttcg agaaaagaaa ttcgatcgag gacgcaaatg gccgctctct
      661 catcaccgac cgcctggcct gcaggcacgc accggcccgc cgcatgggcg cagcgtacgt
      721 ccgcggccaa ccgcgcacat ggttcacatg gcgcacaagg cacgcaaggc atgcatgacg
      781 cccatgggcc gctagccgag cccatgtcca gggcctctgg cgcatccgtg gagcgcgccg
      841 ccgcacgcag ggcctgcgcc ttcgtgttca tcgcgaacct gccgcacctg gccgaagcct
      901 acgggcagga cttcgcatcc gccgcgagcc tcgaggtgca gcgcaggctg tgcgccggct
      961 tcacctcgct gccctcgtcc gacatggcgc ggctgcgcga cgactgcttc ctggtgtggg
     1021 ccaacgaggc cttcgaatgc ggccccttcg gcatccacga agcgggcaag gcatcgggcg
     1081 cctcgcggcg agcgagccac ccggtcgaga cgctgctcgc ggtgctcggt gcggagccgg
     1141 tgcgctccgg cggcatcgcc gcgctggtgc agctgcatgc cgactggatc gacgtgcgct
     1201 cgccgcagca gatgggcgcc tccgagatcg aactcacgct gtggaccgcg cagccctttc
     1261 ccgattcgcg cgagagccgc gccgacggat ggcgccagct ctatcgcgcc gacatggacg
     1321 tcgccgtgcg cgtgagcgaa gcgctgcgcg ccgacgagct ctcgttctcg tggcagccgg
     1381 tggtgcatcc cggcagcgtc gcgaccacgc tgtaccgatc gggccgcgct cgcatcgcac
     1441 ccagtcgcgg cgatgcggcc tcgctcgcgc ccgaagtgtt catgccctgc ctgcagcggc
     1501 tgggcctcac gcgcgccttc gaccgcaccg cgatgcggcg ggccgtggcc gcgctgcggc
     1561 tgcagccttc ggcgcacatc ggcgtgagca tctcggcgca gagcatccag atcgaccact
     1621 ggtgggcttc tctgctcgcg gttctcgaaa gggagcccgc gctggcctcc cgcctggtga
     1681 tcgagatcgc gggcagcgcc tcgctgcccg acctcgaggc cgcgcacgac ttctgcgtgc
     1741 agctgcagat gcgcggctgc cgcatcgccg tccgcgactt cggcggcggc gcggacaacg
     1801 tcgcggccgt gcaggcctgc aagcccgaca tcgtgaagct cgacgcggcc ctgctgcgcc
     1861 gcgcgcgcga cagcgagttc ggccgcgagt gcctgcgcgg aatgctggcg acgagcgcgc
     1921 acttcgcgcc gcacgtcgtc gtcgacggcg tggagcgcga cgacgacgtg cacatcgcag
     1981 ccgacgcggg cgcccagtgg ctgcagggc
//
LOCUS       DATCRO010000841         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_380698, whole genome shotgun sequence.
ACCESSION   DATCRO010000841 DATCRO010000000
VERSION     DATCRO010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380698"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VIN75_22890"
     CDS             complement(<1..355)
                     /locus_tag="VIN75_22890"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8977081.1"
                     /translation="MNTEVLPVPAAPAQAAPRETLSARTLRSGSWTIVGNLAGQALRF
                     GSNLVLTRLLFPEAFGLMAIAQAILTAARLFSDVGLMQGAVRSARGHEQRYLDTIWTL
                     DILKGSLIVLVMTALG"
     gene            complement(601..1785)
                     /gene="pobA"
                     /locus_tag="VIN75_22895"
     CDS             complement(601..1785)
                     /gene="pobA"
                     /locus_tag="VIN75_22895"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY8977082.1"
                     /translation="MRTQVAIVGAGPAGLLLGQLLHEAGVDNVILEARTPEYVLGRIR
                     AGVLEQTTVRLLEQARVAGRLEREGLRHAGTELAFDDRLHRIDFERLTGRCVTVYGQT
                     EVTRDLMDARAAAGRTTFHEAADVSLHHFDGSAPIVRWRQGGVERELRCDFIAGCDGF
                     HGVARASVPAGAIATYEKVYPFGWLGLLAHVPPARHELVYANHERGFALCSQRSATLS
                     RYYVQVPLDDRVEAWSDDAFWDELRRRLPAEVAERVVTGPALEKSIAPLRSFVAEPMR
                     FGRLFLAGDAAHIVPPTGAKGLNLAASDVGLLAAALREHYADRSDAGIDTYSERCLRR
                     VWKAERFSWWFTSLMHRFPGDGAFDRRMQHAELDYLVNSQAASAAMAENYVGLPLELP
                     AA"
     gene            2077..2577
                     /locus_tag="VIN75_22900"
     CDS             2077..2577
                     /locus_tag="VIN75_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977083.1"
                     /translation="MSTTPPDDVAAMFGLDPLPDTSHADAQAAAAAAAEEAALAAELA
                     AAMESGDFADDAPDSRTDLRVKVSWPARMHLPDGRIIELKVRDVSQGGVGLMSEVPIP
                     SSTVVGFELDVPPLEQGGAITPVTGTIRTTYTVTQGSEVIGGATWQAPPTGLPLVIDW
                     IERLRR"
BASE COUNT          365 a          921 c          957 g          389 t
ORIGIN      
        1 ggccgagcgc cgtcatcacc agcacgatca gcgagccctt gaggatgtcc agcgtccaga
       61 tggtgtccag gtagcgctgc tcgtggccgc gcgcgctgcg caccgcgccc tgcatgaggc
      121 cgacgtcgga gaacaggcgc gcggcggtca ggatcgcctg cgcgatggcc atcaggccga
      181 aggcctcggg aaacaggagg cgcgtgagga ccaggttgct gccgaaacgc agggcctggc
      241 ccgcgaggtt gccgacgatc gtccagctgc cgctgcgcag ggtgcgcgcc gacagcgtct
      301 cgcgtggcgc cgcctgtgcc ggcgccgccg gcacgggcag gacttcggtg ttcacggcgg
      361 gctcaggcac tcgcccgcgg gcggcagtgc ggcacgccgg gcgtgaccgg cgtgcgtggc
      421 ctatcgagcg tgcgcggcat gcgcaccccg gcagcgatgg cgtcgtggcg catcgttccc
      481 tccgtatcct tgttatgcgc gaatcctagt gcacaacgcg gtcggatgta accggatgtg
      541 ccgcatgcgg cagcggcaat cgtggcgaaa ggccgctttc cgcgcggcgt cgcgaccgct
      601 tcaagctgcc ggcagctcca gcggcaggcc gacgtagttc tcggccatcg ccgccgaggc
      661 ggcctgcgag ttcaccaggt agtcgagctc ggcgtgctgc atgcgccggt cgaacgcccc
      721 gtcgcccggg aagcggtgca tcagcgacgt gaaccaccac gagaagcgct cggccttcca
      781 gacgcgacgc aggcagcgct ccgaatacgt gtcgatgccg gcatcgctgc gatcggcgta
      841 gtgctcgcgc agcgccgcgg ccagcaggcc gacgtcggac gccgccaggt tcaggccctt
      901 ggcgccggtg ggcggcacga tgtgcgcggc gtcgccggcc aggaacagcc ggccgaagcg
      961 catcggctcg gcgacgaagc tgcgcagcgg cgcgatgctc ttctccagcg cggggccggt
     1021 gaccacgcgc tcggcgacct cggccggcaa ccgccggcgc aattcgtccc agaacgcgtc
     1081 gtccgaccag gcctcgacgc ggtcgtccag cggcacctgc acgtagtagc ggctcagcgt
     1141 cgcgctgcgc tggctgcaca gtgcgaatcc gcgctcgtgg ttcgcataga cgagttcgtg
     1201 tcgcgccggc ggcacgtgcg cgagcaggcc gagccagccg aacggataga ccttctcgta
     1261 ggtggcgatc gcccccgcgg gcacgctcgc gcgcgcgacg ccgtggaaac cgtcgcagcc
     1321 ggcgatgaag tcacagcgca gctcgcgctc gacgccaccc tggcgccagc gcacgatcgg
     1381 cgcgctgccg tcgaagtggt gcagcgacac gtcggccgcc tcgtggaacg tcgtgcggcc
     1441 ggccgcggca cgggcgtcca tcaggtcgcg cgtgacctcc gtctggccgt acacggtcac
     1501 gcagcggccg gtcaggcgct cgaagtcgat gcggtgcaga cggtcgtcga aggcgagttc
     1561 ggtgccggcg tggcgcaggc cctcgcgctc gagtcgaccg gcgacgcggg cctgctcgag
     1621 cagtcgcacg gtggtctgct ccagcacgcc ggcgcggatg cggcccagca cgtactcggg
     1681 cgtgcgggcc tcgaggatga cgttgtcgac gccggcctcg tgcaggagct ggcccagcag
     1741 caggcccgcg gggccggcgc cgacgatggc gacctgggtg cgcatcgggc tcatcgcggg
     1801 cgtcctgtcc tgtcgtcatg catcggcgtc tcctgtacgt ggcgtcgtct cggcagggta
     1861 ggtctgcgcg ggctatcgct caatgcacgg gagttgcgag cggttggact ttcgggacat
     1921 cggcgctcct tctcgtgttt gactggcgcg tcgcctgcgg cgccgcgcgc ctggagcggc
     1981 ggggcgccaa aatcgttcgt attcctcacg cgcgggcacg ctgctccgtg cattcgcgct
     2041 agcatcgatc gatgttcgcc cgctcgtgat ccaaccatgt cgacgacgcc ccccgatgat
     2101 gtcgcggcca tgttcggcct cgatccgctg ccggatacat cgcatgccga tgcgcaggct
     2161 gcggctgccg cggcggccga ggaggcggca ttggcggctg agctcgcggc cgcgatggag
     2221 tcgggggact ttgccgacga cgcgccggac tcgcgcaccg acctgcgcgt caaggtttcg
     2281 tggccggcgc gcatgcacct gcccgacggc cgcatcatcg agctcaaggt tcgcgacgtc
     2341 tcgcaaggtg gcgtgggcct catgagcgag gtgcccatcc cgtccagcac cgtcgtcggc
     2401 ttcgaactgg acgtgccgcc gctcgaacag ggcggcgcca tcacgcccgt cacgggtacg
     2461 atccggacga cctacacggt cacccaaggc tccgaggtca tcggcggcgc cacctggcag
     2521 gcgccgccaa cgggcctgcc gctcgtgatc gactggatcg agcggctgcg acgctgaagc
     2581 cgcgctcgcg ggccggactt caggcctgcg gatccgccgg cgcctggacc tg
//
LOCUS       DATCRO010000842         6863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_470744, whole genome shotgun sequence.
ACCESSION   DATCRO010000842 DATCRO010000000
VERSION     DATCRO010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470744"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3012)
                     /locus_tag="VIN75_22905"
     CDS             complement(<1..3012)
                     /locus_tag="VIN75_22905"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8977084.1"
                     /translation="MTRRLPSLTLLAAALLAGQAAFAALPRFPQPYGDRIVFVADGNL
                     WSVPKAGGTALRLTSAPGQDLMPRVSPDGRWIAYTEAAKAGTDIWVIPSAGGQARRLT
                     FHPPTEPGTGGRHGPDDMVVTWTPDSKSVVFLSKRDQWNSWIQDMYKVPVDGGAAVRM
                     PIDSAVGLATFAPDGHTIAYNRIFTNFRTWKRYNGGQAQQLFSYDFDTRKSTQLTHWS
                     GTNTSPMWVGDKIYYLSDQDAHRRANLWVLDRATGKTREVTHFTDYDIDFPAYGADAI
                     AFQQGGKLWRLDLPGEQLHEVPVSVPDDNPRLRARVVDVKDQIRDSDPAQQVDFALAP
                     NGRRTLFSARGDIFSVPTEHGATRDLTATQGIDEDHPAWSPDGKWVAYTTDVGGNPQV
                     AIRPAEGGAEKVLTAFKEGYLYGPVFAPDGRTLAFSDGAHRLWTVRTDGGAPKMVAQD
                     KLGEIHDQAFSPDGRWLAFSMTAVGRRRDLYLQELATGHVTRLGEGNEIDANPAWSPD
                     GKYLYFTSSRHENPVGSDVDFDFAFLKSGGIYAIALARDTASPVAPKSDEADSPPDKD
                     DAKMDDDAARKKAAKAVKDTANAKGSPADEKQPPDRTAAIAPIRIDLDGLMARAVALP
                     VDAANITQMDVRGHRIFYLTTPLGLIDGQLKGEKSALRFYDLNKRKASVVTEDVDAYS
                     LSLDGERVLVRHDKDYTVLDTKADAAKDEETKKALNLAHLRALVDPAQEWSEMFENAW
                     RLERDLFFSTAMNGVDWKATHDSYRKLLPLAGSREDLNYLIGQMIGEMGNSHTYVGGG
                     DDGETAPAARAGLLGVDWSLDAASGRYRLAHIYAGDNTRDDYRSPIAQPGLNVKAGDY
                     VLAINGAELKAPTDPDALLQLTDADTTVDLTIAPTPDGARRHVVVKPVARELPLREAA
                     WIEHNRALVDKLSGGRVGYVYMSDMEQLGIQQFVRQFYAQLGKQAMIIDDRWNGGGFI
                     APFALERLRRVLVSLGTNREGGLQTEPEEVLH"
     gene            3222..4442
                     /locus_tag="VIN75_22910"
     CDS             3222..4442
                     /locus_tag="VIN75_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977085.1"
                     /translation="MTNSRTAWGVVAAGALITWMAMGALLALPVFLGPIAADTGWSRG
                     GIGLAMTLNFLSMGVASFVWGGLSDRYGPRTILTAGVVLLGIGLALASRSTSLLEFQL
                     IYGGLIGVAAGSLMVPLMSTVTLWFDQHRALAVSLVSSGIGVAPMTVSPFAAELVVTH
                     GWRTSQLILAAAVVVLLVPAALFVRRPPHMPAATAAGGVGGPELKAAARRTLRSRAFI
                     VLSLTFFACCATHAGPIFHTISYAIGCGLPPMTAVTIYSVEGFAGLLGRLLFGVLGDR
                     LGVKRMIVAGLVIQALAAGSYILASALGDFYVVAFVFGMAYGGVMPLYAALARDYFPP
                     QVMGTALGAMTLLSGLGMALGPALGGWIYDHFHAYTFMYLGSLAVGVGAGAIALALPR
                     VRAHAPGAPLAAAA"
     gene            4590..5753
                     /locus_tag="VIN75_22915"
     CDS             4590..5753
                     /locus_tag="VIN75_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977086.1"
                     /translation="MAQHDNGRRNTGQASGAWQQGQRDPQDFPGTQQDQSSGRYGSSY
                     GGGQQGSHHDQGSSLGQHNQWESGRQQQGDNWRERQGPAAGGWRPAMEERDRSWNDRG
                     QGGPSAGYGAQGSSGGQGSFGGQAADNDYGNRGITESGNYGNYGGMAGQGRAGGTYGQ
                     TYGRGGSYDQGGQSAQRFGGYGHGYDARSDSTGGYGTSNFGPDQGVGSGNYGGGDWNA
                     GSYGGGAGRDRTLGGGRGYDQTYGQSSADNYQGQRGWQGQHPSDRDRYGDNGYQSNWQ
                     QGQRGGQAYHDPDYHQWRSEQLNKLDRDYEEWRQHRYQRFSDDFDKWRADRDNQRGAG
                     QGGTESSASSGSTGTGSTGHPTTAGETSGTQAGTSKHDISSTADKGAGKTATK"
     gene            complement(5794..6246)
                     /gene="queD"
                     /locus_tag="VIN75_22920"
     CDS             complement(5794..6246)
                     /gene="queD"
                     /locus_tag="VIN75_22920"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY8977087.1"
                     /translation="MLTITKRLEFDAGHRIPQHKSQCRNLHGHRYALEITLEGEPVDD
                     PGSSDHGMLMDFTDVKTIAKDHLVDLWDHAFLVHDGDTLIRDLLAQIPGHKTVVVDRV
                     PTAENLVAIAFGILDPLYTERFGNHLKLRRLRLYETPTSWADCDRKTL"
     gene            complement(6378..>6863)
                     /gene="queE"
                     /locus_tag="VIN75_22925"
     CDS             complement(6378..>6863)
                     /gene="queE"
                     /locus_tag="VIN75_22925"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY8977088.1"
                     /translation="DTDFVGTDGEGGGKFKTADGLADEIAKHWGDTPSHRFVVLTGGE
                     PLLQVDAALVDALHARMFTIAVETNGTVEPPAGLDWVCVSPKADSKIVITEGDELKLV
                     FPQEGVDPERFADLRFTHFFLQPMDNMLQKTNQRLAMDYCLAHPQWKLGLQTHKILDI
                     R"
BASE COUNT          942 a         2327 c         2541 g         1053 t
ORIGIN      
        1 gtgcagcact tcttcgggct cggtctgcag gccgccctcg cggttggtgc ccagcgagac
       61 cagcacgcgg cgcaggcgct ccagcgcgaa cggcgcgatg aagccgccgc cgttccagcg
      121 gtcgtcgatg atcatcgcct gcttgcccag ctgcgcgtag aactggcgca cgaactgctg
      181 gatgcccagc tgctccatgt cgctcatgta gacgtagccg acgcggccgc ccgacagctt
      241 gtccaccagc gcgcggttgt gctcgatcca cgcggcctcg cgcagcggca gctccctggc
      301 cacgggcttg acgaccacgt ggcggcgcgc gccgtcgggc gtgggcgcga tcgtcagatc
      361 gacggtggtg tcggcgtcgg tcagctgcag cagcgcgtcg ggatcggtgg gcgccttcag
      421 ctcggcgccg ttgatcgcca gcacgtagtc gccggccttg acgttgaggc ccggctgcgc
      481 gatggggctg cggtagtcgt cgcgcgtgtt gtcgccggca tagatgtgcg cgaggcggta
      541 gcggcccgac gcggcgtcca gcgaccagtc gacccccagc aggccggcgc gcgcggcggg
      601 cgcggtctcg ccgtcgtcgc caccgcccac gtaggtgtgg gagttgccca tctcgccgat
      661 catctggccg atcaggtagt tcaggtcctc gcgcgagccc gccagcggca gcagcttgcg
      721 gtagctgtcg tgcgtggcct tccagtcgac gccgttcatg gcggtggaga agaacaggtc
      781 gcgctccagg cgccacgcgt tctcgaacat ctccgaccat tcctgcgccg ggtccaccag
      841 cgcccgcagg tgggcgaggt tcagcgcctt cttcgtctcc tcgtccttgg ccgcgtcggc
      901 cttggtgtcc agcaccgtgt agtccttgtc gtggcgcacc agcacgcgct cgccgtccag
      961 cgacagcgag tacgcgtcca cgtcctccgt caccaccgag gccttgcgct tgttcaggtc
     1021 gtagaagcgc agcgccgact tctcgccctt gagctggccg tcgatcaggc ccagcggcgt
     1081 ggtcaggtag aagatgcggt ggccgcgcac gtccatctgg gtgatgttgg cggcgtccac
     1141 gggcagcgcc acggcgcgcg ccatcaggcc gtcgaggtcg atgcggatcg gcgcgatggc
     1201 cgcggtgcgg tcgggcggct gcttctcgtc ggcggggctg cccttggcgt tggccgtgtc
     1261 cttgacggcc ttggcggcct tcttgcgcgc cgcgtcgtcg tccatcttcg cgtcgtcctt
     1321 gtcgggcggg ctgtcggcct cgtccgactt gggcgccacg ggcgaggcgg tgtcgcgcgc
     1381 gagcgcgatc gcgtagatgc cgccggactt caggaacgcg aagtcgaagt cgacgtccga
     1441 gcccaccggg ttctcgtggc ggctggacgt gaagtacagg tatttgccgt cgggcgacca
     1501 cgcggggttc gcgtcgatct cgttgccctc gcccaggcgc gtgacgtggc cggtggccag
     1561 ttcctgcagg tacaggtcgc gccggcggcc gaccgcggtc atgctgaacg cgagccagcg
     1621 gccatcgggc gagaaggcct ggtcgtggat ctcgccgagc ttgtcctgcg ccaccatctt
     1681 cggcgcgccg ccatcggtgc gcaccgtcca cagccggtgc gcgccgtcgg agaacgccag
     1741 cgtgcggccg tcgggcgcga acaccgggcc gtacaggtag ccctccttga acgccgtgag
     1801 caccttctcg gcgccgcctt cggccgggcg gatcgccacc tgcgggttgc cgccgacgtc
     1861 ggtcgtgtac gccacccact tgccgtcggg cgaccacgcc gggtggtcct cgtcgatgcc
     1921 ctgcgtggcg gtgaggtcgc gcgtggcgcc gtgctcggtg ggcacgctga agatgtcgcc
     1981 gcgcgccgag aacagcgtgc ggcggccgtt gggcgcgagc gcgaagtcga cctgctgcgc
     2041 gggatcggag tcgcggatct ggtccttgac gtcgacgacg cgggcacgca gccgcgggtt
     2101 gtcgtcgggc acgctcaccg gcacctcgtg cagctgctcg cccggcaggt ccaggcgcca
     2161 cagcttgccg ccctgctgga acgcgatcgc gtcggcgccg tacgcgggga aatcgatgtc
     2221 gtagtcggtg aagtgcgtca cctcgcgcgt cttgcccgtc gcgcggtcga gcacccacag
     2281 gttggcgcgg cggtgcgcgt cctggtcgct caggtagtag atcttgtcgc cgacccacat
     2341 cggcgaagtg ttggtgccgc tccagtgcgt gagctgcgtg ctcttgcggg tgtcgaagtc
     2401 gtagctgaac agctgctgcg cctggccgcc gttgtagcgc ttccaggtgc ggaagttggt
     2461 gaagatgcgg ttgtaggcga tggtgtggcc gtcgggcgcg aacgtggcca ggccgaccgc
     2521 gctgtcgatg ggcatgcgca ccgcggcgcc gccgtccacg ggcaccttgt acatgtcctg
     2581 gatccacgag ttccactggt cgcgcttgga caggaacacc acggacttgg agtccggcgt
     2641 ccaggtcacg accatgtcgt cggggccgtg gcggccgccg gtgcccggct cggtcggcgg
     2701 atggaacgtg aggcgccgcg cctggccgcc ggcgctgggg atgacccaga tgtcggtgcc
     2761 ggccttggcg gcctcggtgt aggcgatcca gcggccgtcg ggcgagacgc gcggcatcag
     2821 gtcctggccc ggggcgctgg tcaggcgcag cgcggtgccg cccgccttgg gcaccgacca
     2881 caggttgccg tcggcgacga acacgatgcg gtcgccgtac ggctggggaa agcgcggcag
     2941 cgccgcgaag gcggcctggc cggccagcag ggcagcggcg agaagggtca gcgaaggcag
     3001 gcgacgagtc attccgaggc ttcctggtcg aagaaaagag gtggcgcaag gtaaccgatc
     3061 acggcgggcc ctggcgcgtc cggggagacg aaatgccgga tcacggtcgt gggcgacgcc
     3121 cgcgccacgg ccgcgcaagc ttgccgacgg gttcgcggcg attcgcggcg cttgtcgaaa
     3181 acccgctgcc ccgttcgtcg tggaggcagg aggatcccgg aatgacgaac tctcgaaccg
     3241 cctggggcgt ggtcgcggcg ggcgcgctga tcacctggat ggccatgggc gcgctgctgg
     3301 cgctgcccgt gttcctgggg ccgatcgcgg ccgacaccgg ctggtcgcgc ggtggcatcg
     3361 gcctggcgat gacgctgaac ttcctgtcga tgggcgtggc gtccttcgtc tggggcgggc
     3421 tgagcgatcg ctacgggccg cggacgatcc tgacggccgg cgtcgtcctg ctgggcatcg
     3481 gactggcgct ggcgagccgc tcgacctcgc tgctggagtt ccagctgatc tacggcggcc
     3541 tgatcggcgt ggccgcgggc agcctgatgg tgccgctgat gtcgaccgtg accctgtggt
     3601 tcgaccagca ccgggcgctg gctgtgtcgc tggtgtcctc gggcatcggc gtcgcgccga
     3661 tgacggtgtc gccgttcgcg gcggagctgg tcgtcacgca cggctggcgc acgtcgcagc
     3721 tgatcctggc cgcggccgtg gtcgtgctgc tggtgccggc cgcgctgttc gtgcgccggc
     3781 cgccgcacat gccggccgcc accgccgccg gcggcgtggg cgggcccgag ctgaaggccg
     3841 ccgcgcggcg cacgctgcgt tcgcgcgcgt tcatcgtgct gtcgctgacc ttcttcgcct
     3901 gctgcgccac gcacgcgggg ccgatcttcc acacgatcag ctacgcgatc ggctgcgggc
     3961 tgccgcccat gacggccgtc accatctaca gcgtcgaggg tttcgcgggc ctgctcgggc
     4021 gcctgctgtt cggcgtgctg ggcgaccggc tgggcgtcaa gcgcatgatc gtcgcgggcc
     4081 tggtcatcca ggcgctggcg gccggctcct acatcctcgc gtcggcgctg ggcgacttct
     4141 acgtggtcgc gttcgtcttc ggcatggcct acggtggcgt gatgcccctg tacgcggcgc
     4201 tggcgcgcga ctacttcccg ccgcaggtca tgggcaccgc gctgggcgcg atgacgctgc
     4261 tgtcgggcct gggcatggca ctcggcccgg cgctcggcgg ctggatctac gaccacttcc
     4321 acgcgtacac gttcatgtac ctgggctcgc tggccgtggg cgtgggcgcc ggcgcgatcg
     4381 cgctcgcgct gccgcgcgtg cgcgcgcacg cgcccggcgc gccgctggct gccgcggcct
     4441 gagccgctgg acgaacgatc gcctcgggcg cattcgaaac tcgaatcgcg cgagcgctcg
     4501 acggggcatc ggcgttgccg cattcggggt tccgaccccg gcctcccgtg gccggcgacc
     4561 gaggacttca ccgcaagagg aatgcgatca tggctcaaca cgacaacgga cggcgcaaca
     4621 ccggccaggc atccggcgcc tggcagcaag gacagcgcga cccgcaggac ttcccgggca
     4681 cccagcagga ccagtcgagc ggccgctacg gcagcagcta tggcggcggc cagcagggca
     4741 gtcaccacga ccagggcagc tcgctcggcc agcacaacca gtgggaatcg ggccgccagc
     4801 agcagggcga caactggcgc gagcgccagg gccccgccgc gggcggctgg cgccccgcca
     4861 tggaagagcg tgaccgcagc tggaacgacc gcggccaggg cggcccgtcc gccggctatg
     4921 gcgcccaggg cagctccggc ggacagggca gcttcggcgg ccaggccgcg gacaacgact
     4981 acggcaaccg cggcatcacc gagagtggca attacggcaa ctacggcggc atggccggcc
     5041 agggccgcgc gggcggcacc tatggccaga cctacggccg cggcggcagc tacgaccagg
     5101 gcggccagag cgcgcagcgc ttcggcggat acggccacgg gtacgacgcc cgcagcgact
     5161 cgaccggcgg ttacggcacg agcaacttcg gccccgacca gggcgtgggc tcaggcaact
     5221 acggcggcgg cgactggaac gcgggcagct acggcggcgg cgccggacgc gaccgcaccc
     5281 tgggaggcgg ccgcggatac gaccagacgt acggccaatc gtcggcggac aactaccagg
     5341 gccagcgcgg ctggcagggc cagcatccgt ccgaccgcga ccgctacggc gacaacggct
     5401 accagtccaa ctggcagcag ggccagcgcg gcggccaggc gtaccacgac cccgactacc
     5461 accagtggcg cagcgagcag ctgaacaagc tcgaccgcga ctacgaggag tggcgccagc
     5521 atcgctacca gcgcttctcg gacgacttcg acaaatggcg cgccgaccgc gacaaccagc
     5581 gcggcgccgg ccagggcggc acggagtcga gcgcgtcgag cggcagcacg ggcaccggct
     5641 cgaccggcca cccgacgacc gccggcgaga cctcgggcac gcaggcgggc acgtccaagc
     5701 acgacatctc gagcaccgcc gacaagggcg ccggcaagac cgcaacgaag tgagcgcggc
     5761 aggcggccgc gcgcgagcgg ggccgccctg cgcctacagc gtcttgcggt cgcagtccgc
     5821 ccagctggtg ggcgtctcgt acagccgcag gcggcgcagc ttcaggtgat tgccgaagcg
     5881 ctcggtgtag agcgggtcga ggatgccgaa cgcgatcgcc acgaggttct cggcggtggg
     5941 cacacggtcg accaccaccg tcttgtggcc ggggatctgc gcgagcaggt cgcggatcag
     6001 cgtgtcgccg tcgtgcacca ggaacgcgtg gtcccacagg tcgacgaggt ggtccttggc
     6061 gatggtcttc acgtcggtga agtccatcag catgccgtga tcactcgacc cgggatcgtc
     6121 gaccggctcg ccctcgagcg tgatctccag ggcgtagcga tggccatgca ggttgcggca
     6181 ctggctcttg tgctgcggga tgcggtggcc ggcgtcgaat tcgagtcgtt tggtgatggt
     6241 cagcatgatg ggatcgagac ctggtggcgc accggatcag gacatcggag aacgcccgga
     6301 cgccgcgccc ggccgctccg gagcaaggcc gggcaagccc ctcgggggcg tcgagcggag
     6361 cgagggggct cacacgttca tcggatgtcg aggatcttgt gcgtctgcag gccgagtttc
     6421 cattgaggat gcgccaggca gtagtccatg gcaaggcgct ggttggtctt ctgcagcatg
     6481 ttgtccatgg gctgcaggaa gaaatgcgtg aagcgcaggt cggcgaagcg ttccggatcg
     6541 acgccctcct gcgggaacac cagcttgagt tcgtcgcctt cggtgatgac gatcttcgag
     6601 tcggccttgg ggctgacgca gacccagtcg aggccggcgg gcggctcgac ggtgccgttg
     6661 gtctccacgg cgatggtgaa catgcgcgcg tgcagcgcgt cgaccagcgc cgcatcgacc
     6721 tgcagcagcg gctcgccgcc ggtgagaacg acgaagcggt gcgagggcgt gtcgccccag
     6781 tgcttggcga tctcgtcggc gaggccgtcg gccgtcttga acttgccgcc gccctcgccg
     6841 tcggtgccga cgaaatcggt gtc
//
LOCUS       DATCRO010000843         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_200818, whole genome shotgun sequence.
ACCESSION   DATCRO010000843 DATCRO010000000
VERSION     DATCRO010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200818"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>2630)
                     /gene="tssH"
                     /locus_tag="VIN75_22930"
     CDS             complement(173..>2630)
                     /gene="tssH"
                     /locus_tag="VIN75_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEY8977089.1"
                     /translation="VYGTLQFGESAVRTGLLVVGAVKTPNLRNALYALSPEFRKLKAD
                     DLSDNFAKIVAGSPEDTLAARDGSNVGGGAAPGEASGAIAPAQMGKQEALKKFTVDLT
                     AQARTGKMDPIVGRDDEIRQVVDILMRRRQNNPILVGEAGVGKTAVVEGFAQRIVRGD
                     VPPSLKEVQLLALDVGLLQAGASMKGEFEQRLRSVIDEVQASPKPIILFIDETHTLVG
                     AGGAAGTGDAANLLKPALARGQLRTIGATTWAEYKKHIEKDPALTRRFQNVQVDEPSE
                     AKAILMMRGVASTMEKHHKVQILDEALEAAVKLSHRYIPARQLPDKSVSLLDTASARV
                     AISLHAVPAEVDDSQRRIEALETEIGIIAREAAIGIPTAEREEAVNALLATEKERLAT
                     LTKRWNDEKTLVDRLLSLRAKLRDGVNPVEGTGSALEASAEKAAQASEATLSEGGAAP
                     KLTAGERADVMTELRGVQDELTKLQGESPLMLPTVDFQAVASVVGDWTGIPVGRMAAN
                     EIDTVLKLPSLLGKRVIGQDHAMEMISKRIQTSRAGLDNPNKPIGVFMLAGTSGVGKT
                     ETALALAEALYGGEQNLITINMSEYQEAHTVSSLKGAPPGYVGYGEGGVLTEAVRRKP
                     YSVVLLDEVEKAHSDVHELFFQVFDKGWMEDGEGRQIDFKNTLILLTTNAGTEMIANL
                     CKDPDLMPDPEGMAKAIREPLLKIFPPALLGRLVTIPYYPLSDEMLGKIVELQLNRIK
                     KRVEARYKMPFNVDGDVVKLVVSRCTESESGGRMIDAILTNTMLPEISREFLERMMKG
                     EAIKGVKVGVRDNEFSYSFD"
BASE COUNT          339 a          907 c          911 g          473 t
ORIGIN      
        1 gcggtgcgcg gctggtcatg ggcccaactc cttggcgatc acgtcgccga ttcggcggcc
       61 agcgcgcgga cggcgggaaa cgacggtcgg tcgatctcgc ggacggcgcc agcgtcgatg
      121 ctgcgacccg gcgcgcacgc ccgtcgagcg ccggctcgcc tccgggggac gctcagtcga
      181 acgagtagct gaactcgttg tccctgacgc ccaccttcac gcccttgatg gcctcgccct
      241 tcatcatgcg ctcgaggaac tcgcgcgaga tctccggcag catggtgttg gtgaggatcg
      301 catcgatcat gcggccgccc gactcgctct cggtgcagcg gctgacgacc agcttgacga
      361 cgtcgccgtc gacgttgaac ggcatcttgt agcgcgcctc gacgcgcttc ttgatgcgat
      421 tgagctgcag ttccacgatc ttgcccagca tctcgtcgga cagcgggtag tacgggatcg
      481 tgacgagacg acccagcagc gcaggcggga agatcttgag caggggctcg cggatcgcct
      541 tggccatccc ttcgggatcg ggcatcaggt ccggatcctt gcagaggttg gcgatcatct
      601 ccgtgccggc gttggtcgtg agcaggatca gcgtgttctt gaagtcgatc tggcggcctt
      661 cgccgtcctc catccagccc ttgtcgaaga cctggaagaa cagctcgtgc acgtccgagt
      721 gcgccttctc gacctcgtcg agcagcacga cgctgtacgg cttgcgacgc acggcctcgg
      781 tcagcacccc gccctcgccg tagccgacgt agcccggagg ggcgcccttg agcgacgaga
      841 ccgtgtgggc ctcctggtac tcgctcatgt tgatggtgat caggttctgc tcgccgccgt
      901 acagcgcctc ggccagcgcg agcgccgtct cggtcttgcc gacgcccgag gtgccggcca
      961 gcatgaacac gccgatcggc ttgttcgggt tgtcgaggcc ggcgcgcgag gtctggatgc
     1021 gcttggagat catctccatc gcgtgatcct ggccgatgac gcgcttgccc agcagcgaag
     1081 gcagcttcag cacggtgtcg atctcgttgg cggccatgcg gcccaccggg atgcccgtcc
     1141 agtcgccgac gacgctggcc accgcctgga agtccaccgt cggcagcatc agcgggctct
     1201 cgccctggag cttggtgagc tcgtcctgca cgccgcgcag ctcggtcatg acgtcggcac
     1261 gctcaccggc agtgagcttg ggtgcggctc cgccttcgga cagcgtcgcc tcactggcct
     1321 gcgcggcctt ctcggcgctc gcctccagcg cgctgccggt gccctcgacc gggttcacgc
     1381 cgtcgcgcag cttggcgcgc agcgacagca ggcgatcgac cagcgtcttc tcgtcgttcc
     1441 agcgcttggt gagcgtggcg aggcgctcct tctcggtggc cagcagcgcg ttgacggcct
     1501 cctcgcgttc ggcggtcggg atgccgatgg ccgcctcgcg ggcgatgatg ccgatctcgg
     1561 tctccagcgc ctcgatgcgg cgctgcgagt cgtcgacctc ggccggcacc gcgtgcaggc
     1621 tgatggccac gcgcgcggac gcggtgtcga gcaggctgac cgacttgtcc ggcagctggc
     1681 gcgccgggat gtagcggtgc gacagcttga ccgcggcctc cagcgcctcg tccaggatct
     1741 gcaccttgtg atgcttctcc atcgtgctgg cgacgccgcg catcatcagg atggccttgg
     1801 cctcgctggg ctcgtcgacc tgcacgttct ggaagcggcg ggtgagcgcc ggatccttct
     1861 cgatgtgctt cttgtactcg gcccaggtgg tggcgccgat ggtgcgcagc tggccgcgcg
     1921 cgagcgcggg cttgagcagg ttggccgcgt cgccggtgcc cgcggcgccg ccggcgccca
     1981 ccagcgtgtg cgtctcgtcg atgaacagga tgatgggctt ggggctggcc tgcacctcgt
     2041 cgatgaccga gcgcaggcgc tgttcgaact cgcccttcat gctggcgccg gcctgcagca
     2101 ggccgacgtc gagcgccagc agctgcactt ccttcaggct gggcggcacg tcaccgcgca
     2161 cgatgcgctg cgcgaagcct tcgacgaccg ccgtcttgcc gacgccggcc tcgccgacca
     2221 ggatggggtt gttctggcgc cggcgcatca ggatgtcgac gacctggcgg atctcgtcgt
     2281 cgcggccgac gatcgggtcc atcttgcccg tgcgcgcctg cgcggtgagg tcgacggtga
     2341 acttcttcag cgcctcctgc ttgcccatct gcgccggggc gatcgcgccg ctggcctcgc
     2401 cgggcgcggc gccgccgccg acgttggagc cgtcgcgcgc ggcgagcgtg tcctccggag
     2461 agccggcgac gatcttcgcg aagttgtccg acaggtcgtc ggccttgagc ttgcggaact
     2521 ccggggacag cgcgtagagc gcgttgcgca ggttgggcgt cttcacggcg cccacgacca
     2581 gcaggccggt gcgcaccgcg ctctcgccga actgcagcgt gccgtagacc
//
LOCUS       DATCRO010000844         7615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_403225, whole genome shotgun sequence.
ACCESSION   DATCRO010000844 DATCRO010000000
VERSION     DATCRO010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403225"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="acpP"
                     /locus_tag="VIN75_22935"
     CDS             complement(<1..230)
                     /gene="acpP"
                     /locus_tag="VIN75_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8977090.1"
                     /translation="MSDIEARVKKIIAEQLGVPEADVANDKAFVADLGADSLDTVELV
                     MALEDEFSIEIPDEEAEKITTVQLAIDYAKAHA"
     gene            complement(329..1081)
                     /gene="fabG"
                     /locus_tag="VIN75_22940"
     CDS             complement(329..1081)
                     /gene="fabG"
                     /locus_tag="VIN75_22940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8977091.1"
                     /translation="MSDLNDGNVQTALVTGASRGIGRAIARTLAERGYRVIGTATSDG
                     GAEAITEALAGNAGCKGVKLDVNDAAGVTALVEGIVKEYGGLHVLVNNAGITRDGLAM
                     RMKDDDWDAVHDTNLKSVFRMSREVMRPMMKQRFGRIINITSVVGASGNPGQANYAAA
                     KAGVAGMTRALARELGSRNITVNCVAPGFIETDMTDVLPEAQKAALLQSIPLARLGKP
                     EEIAQAVAFLASREAGYITGTELHVNGGMYMS"
     gene            complement(1140..2078)
                     /gene="fabD"
                     /locus_tag="VIN75_22945"
     CDS             complement(1140..2078)
                     /gene="fabD"
                     /locus_tag="VIN75_22945"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8977092.1"
                     /translation="MTAFAFVFPGQGSQSVGMLDAWGDHPAVAATLEEASAALGEDIG
                     RLIHEGPKEQLDLTTNTQPVMLTAGIAAWRAWQAEGGASPVAVAGHSLGEYAALVAAG
                     VLSLADALPLVRLRATAMQEAVPVGVGAMAAVLGLDAQAVRDGCKAAAEASGEVVEAV
                     NFNDPKQTVIAGSKAGVDKACELLKAAGAKRALLLPVSAPFHSSLMKPAAERLQAALQ
                     RVTFNTPQIPVVNNIDVATPTDAAAIRDALVRQAAGPVRWVEVVQALRARGATHVIEC
                     GPGKVLAGMAKRIDGELVTGAIYDPATLAEVKGVLA"
     gene            complement(2158..3141)
                     /locus_tag="VIN75_22950"
     CDS             complement(2158..3141)
                     /locus_tag="VIN75_22950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8977093.1"
                     /translation="MTAVRHSRIAGTGSELPPLRLTNDDLVKMLARRGVETSDDWIVE
                     RTGIRARHFVADDLHASDLATAAARRALEAAGCTAADVDLIIVATSTPDMIFPSTASI
                     VQRKLGVHGCAAFDVQAVCSGFIYALSVADAMIKAGSASRALVIGAEVFSRLLDFDDR
                     TTCVLFGDGAGAIVLEASDQPGVVATKLHADGRYLDILCTPGTVSGGKVFGDPLLKMD
                     GPAVFKLAVGVLGDVALEVLELAGRATSDIDWLVPHQANIRIMQGTARKLKLPVDRVV
                     VTVDEHGNTSAASIPLALDVAIRSGQIRRGQRLLLEGVGGGFTWGAALVDY"
     gene            complement(3264..4337)
                     /gene="plsX"
                     /locus_tag="VIN75_22955"
     CDS             complement(3264..4337)
                     /gene="plsX"
                     /locus_tag="VIN75_22955"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY8977094.1"
                     /translation="MTPRSRVRLAVDCMGGDHGPSVTLPACKAFLDSHPDAELLLVGS
                     PGALAPATDWPRCTLITASQVVTMDDTVETALRKKRDSSMRVAIEQLRPNDAGAIAAD
                     VCVSAGNTGALMAVSRYLLKTMDGIDRPAIATFMPNIQDRFTCVLDLGANVDCSAEHL
                     MQFAVMGSALVTATEGRPEPVVGLLNIGEEMIKGSEVIKRAGELLRAVASHGHLNYLG
                     NVEGDDIFKGTVDVVVCDGFVGNVALKSNEGVAKMMKSMIRQEFERSWLTRLAALVAM
                     PVLRRFQARVDPRRYNGAALLGLKGLVFKSHGGADAFSFGQALIRAHDAARNRLLDGV
                     HDAIVDTMQALPPTGGESVPQAA"
     gene            complement(4677..4859)
                     /gene="rpmF"
                     /locus_tag="VIN75_22960"
     CDS             complement(4677..4859)
                     /gene="rpmF"
                     /locus_tag="VIN75_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8977095.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALTTPGTAIEATTGEVHLRHHISP
                     TGFYRGRKVLKTKADA"
     gene            complement(5112..6293)
                     /gene="metK"
                     /locus_tag="VIN75_22965"
     CDS             complement(5112..6293)
                     /gene="metK"
                     /locus_tag="VIN75_22965"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8977096.1"
                     /translation="MANDFLFTSESVSEGHPDKVADQISDAILDAIFTQDPLSRVAAE
                     TLCNTGLVVLAGEITTQANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSN
                     DIAQGVDRASDDYLNLGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
                     LPFLRPDAKSQVTMRYVDGKPHSIDTVVLSSQHAPEMSDGAKMKPEFTEAVIEEIIKP
                     VLPAEWLKDTRYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPT
                     KVDRSAAYAARYVAKNIVKAGLARQCQIQVAYAIGVARPMNVTVYTEGTGVIPDAKLA
                     ELVTELFDLRPKGIIQMLDLLRPIYAKTAAYGHFGREEPEFSWERTDKAQALRAAAGL
                     K"
     gene            complement(6481..7110)
                     /locus_tag="VIN75_22970"
     CDS             complement(6481..7110)
                     /locus_tag="VIN75_22970"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY8977097.1"
                     /translation="MTSPALDPLRLNVAHFAADAQDASGDWAVAELPRLADSECPLDA
                     GSPLKGGVSADSPRLPNRAERDPSRRVRWHAVGSLREVGGEDQVWLHLQAGADVVLQC
                     QRCLLPMTLPVEVDRHFRFVADEDTAAVLDDEIEDEVLVLARSLNLRDLVEDEMLLAL
                     PLVPRHDVCPEAVPMQFGDVEAVEEKANPFASLAILRKDRSGDGGPDIA"
     gene            7175..>7615
                     /locus_tag="VIN75_22975"
     CDS             7175..>7615
                     /locus_tag="VIN75_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8977098.1"
                     /translation="MSQLPRLILASTSPYRRELLERLRLPFEAMSPETDETPLPGETP
                     APLAERLALAKARALSARFPDAVVIGADQVADVDGVAIGKPGTHERAVAQLRAMSGRT
                     IVFQTALAVVRESTGFAQVRRAPVSVRFRALSDDEIETYLRTEQP"
BASE COUNT         1075 a         2548 c         2759 g         1233 t
ORIGIN      
        1 gcgtgggcct tggcgtagtc gatggcgagc tgcaccgtcg tgatcttctc ggcctcttcg
       61 tccgggatct cgatgctgaa ttcgtcttcc agcgccatca ccagttcgac ggtgtcgagc
      121 gagtccgcgc ccaggtcggc cacgaaggcc ttgtcgttgg ccacgtcggc ctcggggacg
      181 ccgagttgtt cggcaatgat tttcttgact cgtgcttcga tatcgctcat gacactcctc
      241 cgggagggtt tgcgtaaaaa cgggcggatt ctacttgccc agataaccag acggccggac
      301 cagccttgcg gccgatacgg caatccgctc aactcatgta catgccgccg ttgacgtgca
      361 gctcggtacc ggtgatgtag ccggcctcgc gcgaggccag gaacgccacg gcctgcgcga
      421 tctcctcggg cttgccgagg cgggccagcg ggatcgactg cagcagcgcg gccttctggg
      481 cctcgggcag gacgtccgtc atgtccgtct cgatgaagcc gggggccacg cagttgaccg
      541 tgatgttgcg gctgcccagt tcgcgggcga gcgcgcgcgt catgcccgcg acgccggcct
      601 tggccgcggc gtagttggcc tggccgggat tgccgctggc gcccacgacc gacgtgatgt
      661 tgatgatgcg gccgaagcgc tgcttcatca tcgggcgcat gacctcgcgc gacatgcgga
      721 agacggactt caggttggtg tcgtgcaccg catcccagtc gtcgtccttc atgcgcatgg
      781 ccaggccgtc gcgcgtgatg ccggcgttgt tgaccagcac gtgcaggccg ccgtattcct
      841 tgacgatgcc ctcgacgagc gccgtgacgc cggcggcgtc gttgacatcg agcttgacac
      901 ccttgcagcc cgcgttgccg gccagcgcct cggtgatcgc ctcggcgccg ccgtcgctgg
      961 tggcggtgcc gatgacgcgg tagccgcgct cggccagcgt cctggcgatg gcgcggccga
     1021 tgccgcggga ggcgccggtg accagcgcgg tctggacgtt gccgtcgttg agatcgctca
     1081 ttcgaaaacc tccgcgccgt gcgctgcggt caattcgccc ttcgggcggc cgtgcgggct
     1141 catgccagga ctcccttcac ttcggccagc gtggccgggt cgtagatcgc gccggtgacc
     1201 agctcgccgt cgatgcgctt ggccatgccg gccagcacct tgcccgggcc gcactcgatc
     1261 acgtgcgtgg cgccgcgcgc gcgcagcgcc tgcaccacct cgacccagcg cacggggccg
     1321 gcggcctggc gcaccagcgc gtcgcggatc gcggcggcgt ccgtgggcgt ggccacgtcg
     1381 atgttgttga cgaccgggat ctgcggggtg ttgaacgtca cgcgctgcag cgcggcctgc
     1441 aggcgctcgg ccgcgggctt catcaggctg gaatggaacg gcgcggacac cggcagcagc
     1501 agcgcgcgct tggccccggc ggccttgagc agttcgcagg ccttgtcgac gccggccttc
     1561 gagccggcga tgacggtctg cttggggtcg ttgaagttga cggcctcgac gacctcgccg
     1621 ctggcctcgg ccgcggcctt gcagccgtcg cgcacggcct gcgcgtccag gcccaggacg
     1681 gcggccatcg cgcccacgcc cacgggcacc gcttcctgca tggcggtggc gcgcaggcgc
     1741 accagcggca gcgcgtcggc cagcgacagc acgccggcgg ccaccagcgc ggcgtattcg
     1801 cccagcgagt ggccggccac ggccaccggc gaggcgccgc cctcggcctg ccacgcgcgc
     1861 cacgcggcga tgccggcggt cagcatcacg ggctgcgtgt tggtggtgag gtccagctgt
     1921 tccttcgggc cttcgtggat caggcggccg atgtcctcgc cgagcgccgc ggaggcttcc
     1981 tcgagcgtgg ccgcgacggc cgggtggtcg ccccaggcgt cgagcatgcc cacgctctgc
     2041 gagccctggc ctggaaagac gaatgcgaat gcggtcatgg gaggggaatg atcttgttgt
     2101 tctctcgggg gcggcccggc aatcgggccg cctgtcgcgg ggagacgggt ggggtggtca
     2161 gtagtcgacg agggcagcgc cccaggtgaa gccgccaccc acgccttcga gcagcaggcg
     2221 ctggccgcgc cggatctggc ccgagcggat cgcgacgtcg agcgccagcg ggatcgacgc
     2281 cgccgacgtg ttgccgtgtt cgtccaccgt gaccaccacg cggtcgacag gcagcttcag
     2341 cttgcgggcc gtgccctgca tgatacggat gttcgcctgg tgcggcacga gccaatcgat
     2401 gtcggaagtg gcccgtccgg ccagctcgag cacttcgagc gccacgtcgc ccagcacgcc
     2461 gaccgccagc ttgaagacgg ccgggccgtc catcttgagc agcgggtcgc cgaacacctt
     2521 gccgccgctg acggtgccgg gcgtgcacag gatgtccagg tagcggccgt cggcatgcag
     2581 cttggtggcc acgacgccgg gctggtcgct ggcctccagc acgatggcgc cggcgccgtc
     2641 gccgaacagc acgcaggtgg tgcggtcgtc gaagtcgagc aggcgcgaaa acacctcggc
     2701 gccgatgacc agcgcgcggc tggccgagcc ggccttgatc atcgcgtcgg ccacgctcag
     2761 cgcgtagatg aagcccgagc acacggcctg cacgtcgaac gccgcgcagc cgtgcacgcc
     2821 gagcttgcgc tgcacgatgc tggccgtcga cgggaagatc atgtcgggcg tggacgtggc
     2881 cacgatgatg agatcgacgt cggccgcggt gcagcccgcg gcctcgaggg cgcgcctggc
     2941 ggcggcggtg gccaggtcgc tcgcgtgcag gtcgtcggcg acgaaatggc gcgcgcggat
     3001 gccggtgcgc tcgacgatcc agtcgtcgga ggtctcgacc ccgcggcggg ccagcatctt
     3061 gaccaggtcg tcgttggtca ggcgcagcgg cggcagctcg ctgccggtgc cggcgatacg
     3121 ggaatggcgg acggcggtaa tttgagagta ctccccaatt ccaggctcaa cgcgagccgc
     3181 ggcatgcgac gggcgcgccg ctcatttgac taccctacgt gcttcgcact tcggtatgag
     3241 cgtttcggag cggccgggcg cgctcatgcg gcttgcggaa ccgattcgcc gccggtgggc
     3301 ggcagggcct gcatggtgtc gacgatggcg tcgtggacgc cgtcgagcaa acggttgcgg
     3361 gcggcatcat gggcgcggat gagcgcctgg ccgaacgaga acgcgtcggc gccgccgtgg
     3421 ctcttgaaga ccaggccctt gaggcccagc agcgcggcgc cgttgtagcg gcgggggtcg
     3481 acgcgcgcct ggaagcggcg cagcaccggc atcgccacca gcgcggcgag cctggtgagc
     3541 cagctgcgct cgaactcctg gcggatcatc gacttcatca tcttggccac gccctcgttg
     3601 ctcttgagcg cgacgttgcc gacgaagccg tcgcagacca ccacgtcgac ggtgcccttg
     3661 aagatgtcgt cgccctcgac gttgcccagg tagttcaggt ggccgtgcga cgcgacggcg
     3721 cgcagcagct cgccggcgcg cttgatgacc tcgctgccct tgatcatctc ctcgccgatg
     3781 ttgagcaggc cgacgaccgg ctcgggacgg ccctcggtgg cggtgaccag cgcgctgccc
     3841 atcacggcga actgcatcag gtgctcggcg ctgcagtcga cgttggcgcc caggtccagc
     3901 acgcaggtga agcggtcctg gatgttgggc atgaacgtgg cgatcgccgg gcgatcgatg
     3961 ccatccatcg tcttgagcag gtagcgcgac accgccatca gcgcgccggt gttgccggcc
     4021 gagacgcaga cgtcggcggc gatggcgccg gcgtcgttgg ggcgcagctg ctcgatggcc
     4081 acgcgcatcg atgaatcgcg cttcttgcgc agcgccgtct cgaccgtgtc gtccatcgtg
     4141 acgacctggc tcgccgtgat cagcgtgcag cgcggccagt cggtggccgg agcgagcgca
     4201 ccggggcttc cgaccagcag cagttcggcg tcgggatggc tgtccaggaa ggccttgcag
     4261 gccggcagcg tcacggacgg tccgtggtcg ccgcccatgc agtccaccgc caggcgaacg
     4321 cgcgaacgcg gagtcagggg tacgggttga atcacagtcg ggatcgatcg gggcgatcta
     4381 attccagcaa tccgggacca tatcagacgc gcctgtcggc cggccacatg acaacccgaa
     4441 tgaccccgcg ccgcatcgga aacagccgag ccgcgcctgc gcgccggaac gcgagacgcc
     4501 gcaaggcgca tggagatcgt ggggttgggc gcggacgacg gccgcggccc ggggccggaa
     4561 cgctcaccgt gtcttgccgc ctcaggcgcg ccggacccgc ggagcgggtg gcgcggccgg
     4621 gacgatgccg catcgggcat cgcaggcggg cgcgacgagc gctgccgccg gaaggatcag
     4681 gcgtcggcct tggtcttcag gaccttgcga ccacgataga agccggtcgg gctgatgtgg
     4741 tggcgcaggt gcacttcacc cgtggtggcc tcgatggccg tgcccggggt ggtcagcgca
     4801 ttgtgcgaac ggtgcatgcc gcgcttggag ggcgacttct tgttttgctg gacagccatt
     4861 tcgatactcc tgggaacgcc accggaaggc cggcgaccgt tctgtgaacc catgcccttg
     4921 ctgcggatcc gaagctgcaa accactgcag cagccgggca aagattgggg aaagccagcg
     4981 attatagcgg cattggggac ggtcgccaag tcggaggggt ctggcctctc ctcggatcgc
     5041 gcctgggtgt tgccgcgagc tgattcgaag ttcgcgatcc gaggcgaagg ccagacccct
     5101 ccggtgccgc tttacttcag tccggcggca gcgcgcagcg cctgcgcctt gtccgtgcgc
     5161 tcccacgaga actccggctc ctcgcggccg aagtggccgt aggcggcggt cttggcgtag
     5221 atcgggcgca gcaggtccag catctggatg atgcccttgg ggcgcaggtc gaacagctcg
     5281 gtgaccagtt cggcgagctt ggcgtcgggg atcacgcccg tgccctcggt gtagaccgtg
     5341 acgttcatcg ggcgcgcgac gccgatcgcg taggccacct ggatctggca ctggcgcgcc
     5401 aggccggcct tgacgatgtt cttggccacg tagcgcgcgg cgtaggcggc cgagcggtcg
     5461 accttggtcg gatccttgcc ggagaacgcg ccgccgccgt gcgggcaggc gccgccgtac
     5521 gtgtcgacga tgatcttgcg gccggtcagg ccgcagtcgc cctgcgggcc gccgacgacg
     5581 aagcggccgg tcgggttgac caggtagcgc gtgtccttga gccattcggc cgggagcacc
     5641 ggcttgatga tctcctcgat gacggcctcg gtgaactcgg gcttcatctt ggcgccgtcg
     5701 ctcatctcgg gcgcgtgctg gctcgacagc acgaccgtgt cgatcgagtg cggcttgccg
     5761 tcgacgtagc gcatcgtgac ctggctcttg gcatccgggc gcaggaacgg caggcggccg
     5821 tccttgcgca gctgggcctg gcgctcgacg aggcggtgcg cgtagtagat cggcgcaggc
     5881 atcagctcgg gcgtctcgtc gcaggcgtag ccgaacatca ggccctggtc gccggcaccg
     5941 aggttcaggt agtcgtcgct cgcgcggtcg acgccctggg cgatgtcgtt gctctgcttg
     6001 tcgtacgcga cgagcacggc gcagcccttg tagtcgatgc cgtactcggt gttgtcgtag
     6061 ccgatgcgct tgatggtgtc gcgcgcgacc tggatgtagt cgacgttggc ctgcgtggtg
     6121 atctcgccgg cgagcacgac caggccggtg ttgcacaggg tctcggcggc cacgcgcgag
     6181 agcggatcct gcgtgaagat cgcgtcgagg atggcgtcgg agatctggtc agcgaccttg
     6241 tccgggtgac cttccgaaac ggattcggaa gtgaagagga agtcattcgc cacgtgtggg
     6301 tgctccagat gcgcgagttc gaggaaatcg cgttcggcgg cctttgcgcg tgggacccgg
     6361 cgaacgcttt agtggaattt gtagtcgccc cacaagttgt tcagttaact cggcgatggg
     6421 gatattgtac gtccatccag catgtccttc ggcggataag ggcggcgatt cccacgcttg
     6481 tcaggcgatg tcggggccac cgtcgccgga cctgtccttg cgcaggatgg ccaggctcgc
     6541 gaacgggttg gccttctcct cgaccgcctc gacgtcgccg aactgcatgg gcacggcctc
     6601 ggggcacacg tcgtggcgcg gcaccagcgg cagcgcgagc agcatctcgt cctcgaccag
     6661 gtcgcgcagg ttcaggctcc tggcgagcac cagcacctcg tcctcgatct cgtcgtcgag
     6721 cacggcggcg gtgtcctcgt cggcgacgaa gcggaagtgg cggtcgacct ccacgggcag
     6781 cgtcatcggc agcaggcagc gctggcactg cagcacgacg tcggcgccgg cctgcaggtg
     6841 cagccagacc tggtcctcgc cgcccacctc gcgcagcgag ccgaccgcat gccagcgcac
     6901 gcggcgcgag gggtcgcgct cggcgcggtt gggcagcctg ggcgagtcgg cgctgacgcc
     6961 gcccttgagc ggcgagcccg cgtccagcgg gcactcgctg tcggccaggc gcggcagctc
     7021 ggcgacggcc cagtcgcccg aggcgtcctg cgcgtcggcg gcgaaatggg cgacgttcag
     7081 gcgcagcgga tcgagggcgg gcgaggtcat gaaatcattc taggccggcg cgacgaggca
     7141 tgttgacgcg cgcgccgcaa gcgacaattc cgcgatgtcc cagttgcccc gcctgatcct
     7201 ggcctccacc tcgccctatc gccgcgagct gctggagcgc ctgcgcctgc ccttcgaggc
     7261 gatgtcgccc gagaccgacg agaccccgct gcccggcgag acgcccgcgc cgctggccga
     7321 gcggctcgcg ctcgcgaagg cgcgcgcgct ctcggcacgc tttcccgacg cggtggtgat
     7381 cggcgccgac caagtggccg acgtcgacgg cgtggcgatc ggcaagcccg gcacgcacga
     7441 gcgcgccgtg gcgcagctgc gcgcgatgag cgggcgcacg atcgtgttcc agaccgcgct
     7501 ggccgtggtg cgcgaatcga ccggcttcgc gcaggtgcgg cgcgcgccgg tgtcggtgcg
     7561 ctttcgcgcg ctgtccgacg acgagatcga gacctacctg cgcaccgagc agccc
//
LOCUS       DATCRO010000845         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_516292, whole genome shotgun sequence.
ACCESSION   DATCRO010000845 DATCRO010000000
VERSION     DATCRO010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516292"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VIN75_22980"
     CDS             complement(<1..630)
                     /locus_tag="VIN75_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8977099.1"
                     /translation="MPSAPTSARPRKRAAPRSYHHGDLRAALLAAAAGWLDEQGAETL
                     TLRELARAAGVSHAAPYHHFASRDELLASVAERAFDRLADALAAAARTPGAAHALLDI
                     GEAYVREALAHPAQFRLMFGPLLARKAEHPGLQRAAERAFVVLLDAATRFAPDRGLEI
                     ALAGWSLSHGAANLAIDGAFGGLPIAPAQARQLARLDAGALVRRMVAGLL"
     gene            704..1162
                     /locus_tag="VIN75_22985"
     CDS             704..1162
                     /locus_tag="VIN75_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8977100.1"
                     /translation="MYHAIVRRKLRRAFADINAGRYDGIVAQFAPRHRHAMPGEHALA
                     GERHTPAATARWYERLQRLLPGLAFEVRAIVVGGMPWRTVATVAWDDRFTLPDGTAGS
                     NTGVHEFELRWGRVRSLVIHCDSQRLHRYCERLVACGVAEAGAAPISDAG"
     gene            1315..>2982
                     /locus_tag="VIN75_22990"
     CDS             1315..>2982
                     /locus_tag="VIN75_22990"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY8977101.1"
                     /translation="MDITNTANLSPAEQALRDAALEYHRSPVRGKIAVTPTKPLSNQR
                     DLSLAYSPGVAYACLAIEQDPALAHDYTARGNLVGVVTNGTAVLGLGDIGPLAGKPVM
                     EGKGCLFQKFAGIDVFDIELAERDPDKLVDIIAALEPTLGGVNLEDIKAPECFYIEKK
                     LRERMNIPVFHDDQHGTAIISTSALINGLELVGKKIGEVRIAVSGAGAAAIACLDLMV
                     ELGVNRANVTVCDSKGVIHDKREDRLDESKKRYQRVTPMRTLADAMQGADVVLGCSAA
                     GAISADMVKSMAARPIILALANPEPEIRPELAKSVRPDCIIATGRSDYPNQVNNVLCF
                     PYIFRGALDCGATRITEAMKIACVKEIADLAKAEISGEVAAAYPGRELRFGPDYLIPT
                     PFDSRLILRIAPAVAKAAESSGVATRPIKDVDGYVQSLGRFVSHTGMLMRPLFSAARA
                     APRRVVYAEGEDERVLRAVQIALDERLVKPILVGRPAVIASRIERAGLRLQAGRDFEI
                     VNPEDDPRFRTYWEAFHKLMARQGVTPEAAKTMVRRSNTTIAALMVHL"
BASE COUNT          447 a         1104 c         1044 g          387 t
ORIGIN      
        1 gagcaggccc gccaccatgc ggcgcacgag ggcgccggcg tccaggcgtg cgagctgccg
       61 tgcctgggcg ggggcgatcg gcaggccccc gaaggcgccg tcgatggcca ggttggcggc
      121 gccgtgcgac aggctccagc cggccagcgc gatctccagg ccccggtccg gcgcgaagcg
      181 cgtggcggcg tccagcagca cgacgaacgc ccgctcggcc gcgcgctgca ggcccggatg
      241 ctcggccttg cgtgccagca gcgggccgaa catcagccgg aattgcgccg ggtgcgccag
      301 cgcctcgcgc acgtaggcct cgccgatgtc cagcagcgcg tgcgcggccc cgggcgtcct
      361 ggcggccgcg gccagcgcgt cggccaggcg gtcgaacgcg cgctcggcca cgctggccag
      421 cagctcgtcg cggctggcga agtggtggta cggcgcggcg tgggacaccc ccgccgcgcg
      481 cgccagctcg cgcagcgtca gcgtttcggc cccctgctcg tcgagccagc cggcggcggc
      541 ggccagcagc gcggcgcgca ggtcgccgtg gtggtacgag cgcggcgcgg cgcgcttgcg
      601 ggggcgggcg gaagtcggcg cggacggcat ggccaatctt gacacgagaa ggatcgagcc
      661 gcacgatgcg ccaatcctga cactgtcaag attggagccc gccatgtatc acgccatcgt
      721 ccgccgcaag ctgcgccgcg ccttcgccga catcaacgcc ggccgctacg acggcatcgt
      781 cgcgcagttc gcgccgcgcc accgccacgc gatgcccggc gagcacgcgc tggcgggcga
      841 gcgccacacg ccggcggcca ccgcgcgctg gtacgagcgg ctgcagcgcc tgctgccggg
      901 gctggcgttc gaggtccgcg ccatcgtcgt gggcggcatg ccctggcgca ccgtcgccac
      961 ggtcgcctgg gacgaccgct tcacgctgcc ggacggcacc gccggctcca acaccggcgt
     1021 gcacgagttc gagctgcgct ggggccgcgt gcgctcgctg gtcatccact gcgactcgca
     1081 gcgcctgcac cgctactgcg aacgactggt cgcctgcggc gtggccgagg ccggcgccgc
     1141 gcccatcagc gacgccggct gaaggcgttc gcaagcgccg cgaaggcccg gctgcccagc
     1201 cgcgaaggct ggcgccacgc cggccgtggg gtcggggcca cagggtttcc atggggttac
     1261 atcaagctgg caagatgcct tccccggtga gcgtgccgga ctgaaggaac catcatggac
     1321 atcacgaaca ccgccaacct gtcgcccgcc gaacaggccc tgcgcgacgc cgccctcgag
     1381 taccaccgca gcccggtgcg cggcaagatc gccgtcacgc ccaccaagcc cctgtccaac
     1441 cagcgcgacc tgtcgctggc ctattcgccc ggcgtcgcct atgcatgcct ggcgatcgag
     1501 caggacccgg cgctggcgca cgactacacg gcgcgcggca acctggtcgg tgtggtcacc
     1561 aacggcacgg cggtactggg cctgggcgac atcggcccgc tggccggcaa gccggtgatg
     1621 gaaggcaagg gctgcctgtt ccagaagttc gccggcatcg acgtgttcga catcgagctg
     1681 gccgagcgcg acccggacaa gctggtcgac atcatcgccg cgctcgaacc cacgctgggc
     1741 ggcgtgaacc tcgaggacat caaggcgccc gaatgcttct acatcgagaa gaagctgcgc
     1801 gagcgcatga acatccccgt cttccacgac gaccagcacg gcacggccat catctcgacc
     1861 tcggcgctga tcaacggcct ggagctggtg ggcaagaaga tcggcgaggt caggatcgcg
     1921 gtctcgggcg ccggcgccgc ggccatcgcc tgcctggacc tgatggttga actgggcgtc
     1981 aaccgcgcca acgtgaccgt ctgcgattcc aagggcgtga tccacgacaa gcgcgaggat
     2041 cgcctggacg aatcgaagaa gcgctaccag cgcgtcacgc ccatgcgcac gctggccgac
     2101 gcgatgcagg gcgccgacgt ggtgctgggc tgctcggcgg ccggcgcgat cagcgcggac
     2161 atggtcaagt cgatggccgc caggcccatc atcctggcgc tggccaaccc cgagccggag
     2221 atccggcccg agctcgcgaa gtcggtgcgc ccggactgca tcatcgccac cggccgctcg
     2281 gactatccga accaggtcaa caacgtcctg tgcttcccgt acatcttccg cggcgcgctc
     2341 gattgcggcg ccacgcgcat caccgaagcg atgaagatcg cctgcgtcaa ggagatcgcc
     2401 gacctcgcca aggccgagat ctccggcgag gtcgccgccg cctacccggg ccgcgagctg
     2461 cgcttcgggc ccgactacct gatccccacg ccgttcgact cgcgcctgat cctgcgcatc
     2521 gcgccggccg tggccaaggc cgccgagtcc tcgggcgtgg ccacgcgccc gatcaaggac
     2581 gtcgacggct acgtgcagtc gctcgggcgt ttcgtgtcgc acacgggcat gctgatgcgc
     2641 ccgctgttct ccgcggcccg cgcggcgccc aggcgcgtcg tctacgccga gggcgaggac
     2701 gagcgcgtgc tgcgcgcggt gcagatcgcg ctggacgaac gcctggtcaa gcccattctc
     2761 gtcggccgcc cggccgtcat cgcctcgcgc atcgagcgcg ccggcctgcg cctgcaggcg
     2821 ggccgcgact tcgagatcgt caaccccgag gacgacccgc gcttccgcac ctactgggag
     2881 gccttccaca agctgatggc gcgccagggc gtgacgcccg aggcggccaa gaccatggtg
     2941 cggcgctcca acacgacgat cgccgcgctg atggtgcatc tg
//
LOCUS       DATCRO010000846         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_357794, whole genome shotgun sequence.
ACCESSION   DATCRO010000846 DATCRO010000000
VERSION     DATCRO010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357794"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VIN75_22995"
     CDS             complement(<1..858)
                     /locus_tag="VIN75_22995"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8977102.1"
                     /translation="MTLRLLAAAGVAVAGLLAGCADDVAKPTPLEPLEAKIAGHEVWK
                     LSLSSFSTLNALAGNAGFGAQGAVAAGDNLIIAALNGVLVSVDAATGKENWRADAGGR
                     LSAGVGSDGRFTAVVTRDGDLVTLDGGKITWRQHLNSAVYTAPFVAGERVFVLGVDRS
                     VQAFDAVDGKRLWVYAKAGDALTLSQAGVLTSYQDTLLFGLGARLTALDPLKGTVRWE
                     VPVAAARGTNEVERLADLVGPAARVGSVYCIRAFQNGIGCVDAERVTALWSTNMSGAQ
                     AIGADQDYVY"
     gene            complement(867..1538)
                     /locus_tag="VIN75_23000"
     CDS             complement(867..1538)
                     /locus_tag="VIN75_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8977103.1"
                     /translation="MAQQLDLQEQEQVDALKAFWAKYGNLIMWAVTLVLAVFAGWNFW
                     QKQVRDKALQASTMYGELKTAADAGDDKRAGQIFGDMKAKYAKATYTQLGGLLAAKVE
                     VDKGDAASAMANLQWVAESGNDENSAVAHLRLAGLLADAKKYDDALKQLAQVKPASFA
                     ALVSDRRGDIEMAQGQKDAAIKAWKVAYDGLPDTAQYRILVEAKLTAQGAAPAASGAA
                     SGAAQ"
     gene            complement(1630..2958)
                     /gene="hisS"
                     /locus_tag="VIN75_23005"
     CDS             complement(1630..2958)
                     /gene="hisS"
                     /locus_tag="VIN75_23005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8977104.1"
                     /translation="MAEILRAVKGMNDIFPASVPLKVDARVTSSNIWQWFEQTARSVL
                     HRYGYQNLMTPIVEPTALFVRGIGEVTDIVEKEMYSFEDSMNGDKLTLRPEFTAGIVR
                     AIIEHNALYNGPVRLWSMGPVFRHERWQKGRYRQFHQLDVEAMGFAGPDVDAEQILLT
                     RTLLRELGLKEGEHVRLEINSLGQPAERAAHRAALVAYFEANATLLDEDSQRRLKTNP
                     LRILDSKNPAMQAMIDAAPKLMDFLGAETLAHFDGVRAVLDGAGLEYRINTRLVRGLD
                     YYNLTVFEWVTEQLGSQGTVCGGGRYDGLIEQLGGKPAPAVGWGLGIERLLLLLEAVG
                     VAPPDRSPDAYVVVGGEEARVQAMVVAEALRARGVSVLQHGQGADGLPGLKAQFKKAN
                     ASGARFALVFAGEELARGMVGLKALRAGESQQVERPLADVASWAAELLNA"
     gene            complement(3043..>3567)
                     /locus_tag="VIN75_23010"
     CDS             complement(3043..>3567)
                     /locus_tag="VIN75_23010"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8977105.1"
                     /translation="GTKGTVASAAALSILLQEGIGDTIRVSLTPQPGEARTQEVVVAL
                     EILQALGLRQFNPSVTACPGCGRTTSTVFQDLTKQIDDFLRSQMPVWRDRFPGVEGMK
                     VAVMGCIVNGPGESKHADIGISLPGTGEAPSAPVYIDGERILTLRGDNIAAEFQAIVE
                     QYIERRYGAATAAH"
BASE COUNT          501 a         1272 c         1234 g          560 t
ORIGIN      
        1 gtagacgtag tcctggtcgg cgccgatcgc ctgcgcgccg ctcatgttgg tcgaccacag
       61 cgccgtcacg cgttcggcgt cgacgcagcc gatgccgttc tggaacgcgc ggatgcagta
      121 gacgctgccc acgcgcgccg ccggcccgac caggtcggcc aggcgctcca cctcgttggt
      181 gccgcgcgcc gcggccaccg gcacctccca gcgcaccgtg cccttgagcg gatcgagggc
      241 cgtcagccgc gcgccgagtc cgaacagcag cgtgtcctgg tacgaggtca gcacgcccgc
      301 ctgcgacagc gtcagcgcgt cgccggcctt ggcgtagacc cacaggcgct tgccgtcgac
      361 ggcgtcgaag gcctgcaccg agcggtcgac acccagcacg aacacgcgct cgccggccac
      421 gaagggggcg gtgtagaccg cgctgttcag gtgctggcgc cacgtgatct tgccgccgtc
      481 cagcgtcacc aggtcgccgt cgcgcgtgac gaccgccgtg aagcggccgt cgctgcccac
      541 gccggcggac agccggccgc ccgcgtcggc gcgccagttc tccttgccgg tggccgcgtc
      601 gaccgacacc agcacgccgt tgagcgccgc gatgatcagg ttgtcgcccg cggcgaccgc
      661 gccctgcgcg ccgaagccgg cgttgccggc cagcgcgttg agcgtgctga agctcgacag
      721 gctcagcttc cagacctcgt ggcccgcgat cttggcctcc agcggctcca gcggcgtcgg
      781 cttggcgacg tcgtcggcgc aaccggccaa cagccccgcg acggcgacac cggcggcggc
      841 caggaggcgc agcgtcacgc ccgccttcat tgcgcggctc cggaagcggc gcccgaggcc
      901 gccggcgcgg cgccctgcgc cgtcagcttg gcctcgacga ggatgcggta ctgcgccgtg
      961 tccggcaggc cgtcgtaggc caccttccag gccttgatgg cggcgtcctt ctggccctgc
     1021 gccatctcga tgtcgccgcg gcggtcggag accagcgccg cgaacgacgc gggcttgacc
     1081 tgcgcgagct gcttgagcgc gtcgtcgtac ttcttggcgt cggccagcag gccggccagg
     1141 cgcaggtggg cgacggccga gttctcgtcg ttgcccgatt cggcgaccca ttgcaggttg
     1201 gccatcgcgc tggcggcgtc gcccttgtcg acctccacct tggcggcgag caggccgccc
     1261 agctgcgtgt aggtggcctt ggcgtacttg gccttcatgt cgccgaagat ctgcccggcg
     1321 cgcttgtcgt cgccggcgtc ggccgccgtc ttgagctcgc cgtacatcgt gctggcctgc
     1381 agcgccttgt cgcgcacctg cttctgccag aagttccagc ccgcgaagac ggccagcacc
     1441 agcgtgaccg cccacatgat caggttgccg tacttggccc agaacgcctt gagggcgtcg
     1501 acctgttctt gttcctggag atcgagttgt tgtgccatcg gaaatcggtc tgtcgtgttt
     1561 cgttgggacg ccggggagcg agcctcggcg ccgggagacg ctgcccggaa aaaccgggcg
     1621 aatcgtcgat tatgcgttga gcagctcggc ggcccaggac gccacgtcgg ccagggggcg
     1681 ctcgacctgc tgcgactccc ctgcgcgcaa ggccttgaga cccaccatgc cgcgcgcaag
     1741 ttcctcgccc gcgaacacca gcgcgaagcg cgcgccgctg gcgttggcct tcttgaactg
     1801 cgccttcagg cccggcaggc catcggcgcc ctgcccgtgc tgcagcaccg acacgccgcg
     1861 ggcgcgcaac gcctcggcca ccaccatcgc ctggacgcgg gcctcctcgc cgcccaccac
     1921 gacgtaggcg tcgggcgacc ggtccggcgg cgcgacgccc acggcctcca gcagcagcag
     1981 caggcgctcg atgcccaggc cccagccgac ggccggcgcc ggcttgccac cgagctgctc
     2041 gatcaggccg tcgtagcggc cgccgccgca cacggtgccc tgcgagccga gctgctccgt
     2101 gacccactcg aacacggtca ggttgtagta gtccaggccg cgcacgaggc gcgtgttgat
     2161 gcggtactcc aggccggcgc catcgagcac ggcgcgcacg ccatcgaagt gcgcgagcgt
     2221 ctcggcgccg aggaagtcca tcagcttggg cgccgcgtcg atcatcgcct gcatcgccgg
     2281 gttcttgctg tccaggatgc gcagcgggtt ggtcttgagg cggcgctgcg aatcctcgtc
     2341 cagcagcgtt gcgttggcct cgaaatacgc caccagcgcg gcgcggtgcg cggcgcgctc
     2401 ggcgggctgg cccaggctgt tgatctcgag gcgcacatgc tcgccttcct tcaggcccag
     2461 ctcgcgaagg agcgtgcgcg tgagcaggat ctgctcggcg tcgacgtccg gaccggcgaa
     2521 gcccatcgcc tcgacgtcca actggtggaa ctggcggtag cggcccttct gccagcgttc
     2581 gtgacggaag accgggccca tggaccacag ccgcaccggc ccgttgtaca gggcgttgtg
     2641 ctcgatgatc gcgcgcacga tgccggccgt gaactccgga cgcagcgtga gcttgtcgcc
     2701 gttcatcgaa tcctcgaagg aatacatctc cttctcgacg atgtcggtca cctcgccgat
     2761 gccacgcacg aacagcgccg tgggctcaac gatcggcgtc atcaggttct ggtagccgta
     2821 gcgatgcagg accgaacggg ccgtctgctc gaaccactgc cagatgttcg aggaggtgac
     2881 gcgcgcgtcg accttcagag ggacggacgc cgggaagatg tcgttcatgc ctttgacggc
     2941 acgcaggatt tcagccatga gcaatacgat gcgggcggcc cggatggggc cgcgagaagg
     3001 gatgtcgcgg gcgcgcgacg acggcgtgag gctggcgcag cgtcaatgcg ctgccgttgc
     3061 tgcgccgtag cgccgctcga tatattgctc gacgatcgcc tggaactcgg cggcgatgtt
     3121 gtcgccgcgc agcgtcagga tgcgctcgcc gtcgatgtag acgggcgcgg acggcgcctc
     3181 gccggtgccc ggcaggctga tgccgatgtc ggcgtgcttc gattcgcccg ggccgttgac
     3241 gatgcagccc atgacggcca ccttcatgcc ctcgacgccc gggaagcggt cgcgccacac
     3301 cggcatctgc gagcgcagga agtcgtcgat ctgcttggtc aggtcctgga acaccgtgct
     3361 ggtggtgcga ccgcagcccg ggcaggccgt gacgctcgga ttgaactggc gcaggcccag
     3421 cgcctgcagg atctcgagcg cgaccaccac ttcctgcgtg cgggcctcgc cgggctgggg
     3481 cgtcagagag acgcggatcg tgtcgccgat gccttcctgc agcaggatcg atagcgccgc
     3541 ggccgacgcc acggtgccct tggtacc
//
LOCUS       DATCRO010000847         6838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_403246, whole genome shotgun sequence.
ACCESSION   DATCRO010000847 DATCRO010000000
VERSION     DATCRO010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403246"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VIN75_23015"
     CDS             <1..1290
                     /locus_tag="VIN75_23015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8977106.1"
                     /translation="GDCAGCHTAAGGTPYAGGRPLATPFGTVLSANLTPDATGLKGWN
                     ADQFHRAMHEGIDDEGKHLYPAFPYNYFARMPRADTDALLAYLQSLAPVAHKVDRNQL
                     PFPFNIRALVSVWNMLYLDKSEFKPDPAHDEQWNRGAYLVEGPGHCAACHTPKTVLGG
                     PKSDHDFQGGAFGTWFAPDLTPNGRTGLGGWSRDEVVQFLRAGRNAHASATGEMGLVV
                     QDSTSLLADDDLQAIASYLSDRPTSPAASMTPPDTAVMKAGEAIFVDQCSACHLMRGQ
                     GQALAFPPLARSANLQQSDPTTALHIILAGAQASPTRAQPTGFTMPAFAWKLDDKQVA
                     AVATYVRNQWGNAAPAVAPDQVAKLRDKLHFDAHTDGRTKATPMTHPGPNTLGAADTD
                     SRDNGSAQAGRAAPAGDAAASAPSKSGSGVDQVQGPG"
     gene            complement(1301..2068)
                     /locus_tag="VIN75_23020"
     CDS             complement(1301..2068)
                     /locus_tag="VIN75_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977107.1"
                     /translation="MKPHASFARLAVAGALFAAPLAHADGLADLKGALARLQAQTPLK
                     ATLDVKTFDRHGQGNDAVEKNGQASIAVEDAARGLQVLYGRDTLARMDSEQRQLARDP
                     NAKTPTVWAIGKLDSSEIVPMTSAASALARRLEEAQFKGEKPDTWNGRAARLLAFSIP
                     VDKLPPDQRKYVKEFDGTLSVWIAADGTPLASETHVAVKGRAFIVVSFDAVDDWNTTY
                     AVLGDRLVALRAENHSSSNGAGEHGEQRVVKTLQPQG"
     gene            complement(2441..2517)
                     /locus_tag="VIN75_23025"
     tRNA            complement(2441..2517)
                     /locus_tag="VIN75_23025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2481..2483),aa:Val,seq:gac)
     gene            complement(2544..3878)
                     /locus_tag="VIN75_23030"
     CDS             complement(2544..3878)
                     /locus_tag="VIN75_23030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY8977108.1"
                     /translation="MARTSSSTLPASGVDPGITEAADRIVRRLLSSVSTAAALRLWDG
                     VTHSFGHGTSRFTLILRDPGVLRDLVLKRGPIPLADAYFRGRLDVEGDLYEALGLKHH
                     FQNLTLSGRERFGLLLDALRLKFSPGGAASPAGDPPATTQFTHDHSMASDRAAISHHY
                     DVSNDFYKLFLDERMVYSCAYFHEDGDSLDQAQANKLDHICRKLRLKPGGRHLDIGCG
                     WGALVIWAAKHYGVHAHGITLSKEQLAEARARIAAEGLQDRVTVELRDYRALEGEGVY
                     DTVSSVGMFEHVGLANLPTYHATVHRVLKPNGLFLNHGITHEEEGWQPSVDTEFINRY
                     VFPDGELDRVSSIQAGMERARFEIHDVENLRQHYAKTLRHWVARLEANHDEAVRHVSE
                     ATYRVWRLYMAASALEFESGGTAIHQILASRRPDARPYAQPVPLSRGDLYAQ"
     gene            complement(4105..4659)
                     /locus_tag="VIN75_23035"
     CDS             complement(4105..4659)
                     /locus_tag="VIN75_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEY8977109.1"
                     /translation="MDQNPYAPPRAVVVESRPEDAPEAPLFAVSIAKMVVLTIATFGL
                     YELVWLYQHWAAIRRRDHSDIWPVPRAIFGVIFCWSLFMRVERAGKEAGVPGGPPFGP
                     LAVAWILSSLCWRLPDPYDWVSMLAPFLTIPVQIYANRVNAVVAPGHDRNARFNWMNI
                     VVLVLGGVFVLLALIGAALPGPAS"
     gene            complement(4695..>6838)
                     /locus_tag="VIN75_23040"
     CDS             complement(4695..>6838)
                     /locus_tag="VIN75_23040"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8977110.1"
                     /translation="AEPLLVGRLLDSGEPALAHAQGVAQILADLGATPAMQAAAWLVY
                     AGDGLQRPIEVVTKAFGPSYASLVDLTRKLVKIQRNAREAQIGKEQRALQTERVRKML
                     LAFSRDLRVVLLRLASRLQTLRWHASSRTPCPPELAAESQQVFAPLANRLGIWQIKWE
                     LEDLSFRFLEPAEYKRVARLLDERRVEREAGIVAFRTQLERMLADAGIHADIQGRPKH
                     IYSIWKKMRGKELDFEHVFDIRAVRVIVDSVADCYAALGRVNERYRAVDGEFDDYIAK
                     PKANGYQSLHTVVKDDKGRAFEVQIRTREMHEHAEHGVAAHWAYKEAGSKGYAGVSAA
                     GAFEDQVAQARKAVLRQLLAWERDFVAEPGAEASPSAGDDAPAFEDRIYVFTPQASVI
                     ELPAGGTPVDFAYALHTDVGHRCRGARVDGVMVPLQTPLQNGQTVEIVTVREGGPSMD
                     WLNPELHFLQSPRARAKVRAWFNALQHAQTVAKGRELVEKLLQREGRTATGLEELAGK
                     LGFDDAQALFEVVGKDEYSLRNIENLLRPAEPPAPDEPIQLKRPRTGGAAGGVLVVGI
                     DSLLTTLASCCRPAPPDAIGGYVTRGKGVAVHRLDCVNFRHMSETNPERVIPVEWGLA
                     AAEGRSGKDVYAVDIVVEAQDRQGLLRDISDVLAKQRVNVIGVASQSTKGIGDRRAFM
                     TFTVEIDSAERLGVVLGLVAKVGGVESARRR"
BASE COUNT         1004 a         2412 c         2404 g         1018 t
ORIGIN      
        1 ggcgactgcg ccggctgcca cactgccgcg ggcggcacgc cctacgccgg cggccgtccg
       61 ctggccacgc cgttcggcac cgtgctgtcg gccaacctca cgcccgacgc cacgggcctc
      121 aagggctgga acgccgacca gttccatcgc gcgatgcacg aaggcatcga cgacgagggc
      181 aagcacctgt acccggcgtt cccgtacaac tacttcgcgc gcatgccgcg cgccgacacc
      241 gacgcgctgc tggcgtatct gcagtcgctg gcgccggtgg cgcacaaggt cgaccgcaac
      301 caactgccgt tcccgttcaa catccgcgcg ctggtgtcgg tgtggaacat gctgtacctg
      361 gacaagagcg agttcaagcc cgacccggcg catgacgagc aatggaaccg cggcgcctac
      421 ctggtcgagg gcccgggcca ctgcgccgcc tgccacacgc ccaagacggt gctcggcggg
      481 cccaagagcg accacgattt ccagggcggc gccttcggca cctggttcgc ccccgacctg
      541 acgcccaacg gccgcaccgg cctgggcggc tggtcgcgcg acgaggtcgt gcagttcctg
      601 cgcgccggcc gcaacgcgca cgcgtcggcc accggcgaga tggggctcgt cgtgcaggac
      661 tccacgtcgc tcctggccga cgacgacctg caggccatcg cgagctacct gtccgaccgc
      721 cccacgtcgc ccgcggcctc gatgacgccg cccgacaccg ccgtcatgaa ggcgggcgag
      781 gcgatcttcg tcgaccagtg ctcggcctgc cacctgatgc gcggccaggg ccaggcgctc
      841 gcgttcccgc cgctcgcgcg ctcggccaac ctgcagcagt ccgacccgac gaccgcgttg
      901 cacatcatcc tggccggcgc gcaggcgtca cccacgcgcg cgcagcccac cggcttcacg
      961 atgcccgcgt tcgcgtggaa gctcgacgac aagcaggtgg ccgccgtggc gacctacgtg
     1021 cgcaaccagt ggggcaacgc cgcgcccgcg gtcgcgcccg accaggtggc caagctgcgg
     1081 gacaagctgc acttcgacgc gcacaccgac ggccgcacga aggcgacgcc gatgacgcat
     1141 ccgggaccca acacgctggg cgcggccgac accgacagcc gcgacaacgg cagcgcccag
     1201 gccggtcgcg cggcgcccgc gggcgatgcg gccgcgagcg cgccgtcgaa gtcgggatcc
     1261 ggcgtcgacc aggtccaggg gccgggctga accgagcccc tcagccctgc ggctgcagcg
     1321 tcttgacgac gcgctgctcg ccgtgttcgc cggcgccgtt gctggacgag tggttctccg
     1381 cgcgcagcgc cacgaggcgg tcgcccagca cggcgtaggt cgtgttccag tcgtccacgg
     1441 cgtcgaaact gacgacgatg aacgcgcggc ccttgacggc gacgtgcgtc tcgctggcca
     1501 gcggcgtgcc gtcggcagcg atccacacgg acagcgtgcc gtcgaactcc ttgacgtact
     1561 tgcgctggtc cggcggcagc ttgtccacgg gaatcgagaa ggccagcagg cgcgcggccc
     1621 tgccgttcca ggtgtcgggc ttctcgccct tgaactgcgc ctcctcgagg cggcgcgcga
     1681 gcgccgacgc ggccgaggtc atcggcacga tctcgctcga gtcgagtttg ccgatcgccc
     1741 agaccgtagg ggtcttcgcg ttcggatcgc gcgcgagctg gcgctgctcg ctgtccatgc
     1801 gcgcgagcgt gtcgcggccg tacagcacct gcaggccgcg tgcggcatcc tcgaccgcga
     1861 tgctcgcctg cccgttcttc tcgacggcgt cgttgccctg gccgtgccgg tcgaaggtct
     1921 tgacgtccag cgtggccttc agcggcgtct gcgcctgcag ccgcgcgagc gcccccttga
     1981 ggtcggccaa cccgtcggca tgggccaggg gcgcggcgaa cagcgcgccg gcgacggcga
     2041 ggcgggcgaa ggaggcgtga ggcttcatcg gtgcggcggc aaggttgatg gcgagggtga
     2101 ccatcgtagc ggccgcttgt tgcacctccg ttgctcgcca acgccgccat cctgcccgcg
     2161 gcgaggcgga atcccaggat cgcaccgtgc gatgcgaggc gatcccggcg ccgccgtgga
     2221 tcccgcgact gcgcgcagga tgacactggg tgggcgcgaa gcggctcagg ctcggcgacg
     2281 cacaaagaaa aagccccgac cgctttcgcg ttcggggctc acttgaattg cagcggactg
     2341 ccttgcagac aggcgccaaa ttctttcgcg gcttcttctg tggggaggat ttctcctcct
     2401 tcggccacat cgatacggca tgccgtgtcg acttcgaatt tggtaggcgc gattggactc
     2461 gaaccaacga cccccaccat gtcaaggtgg tgctctaacc agctgagcta cgcgcctgca
     2521 agaccgctat tatggcatga aatttattgg gcatacaagt cccctcgcga aagcggcacg
     2581 ggttgcgcat acggacgcgc gtcgggacgg cgcgacgcca ggatctggtg gatcgccgtg
     2641 ccgcccgact cgaactccag cgcgctcgcg gccatgtaca ggcgccatac gcgatacgtg
     2701 gcctcgctga cgtgacgcac ggcctcgtcg tggttggcct ccaggcgcgc gacccagtgg
     2761 cgcagcgtct tcgcatagtg ctggcgcagg ttctcgacgt cgtggatctc gaagcgcgcg
     2821 cgctccatgc cggcctggat gctgctgacg cgatcgagtt cgccgtcggg gaacacatag
     2881 cggttgatga actcggtgtc gacgctcggc tgccagccct cttcctcgtg cgtgatgccg
     2941 tggttcagga acaggccgtt cggcttgagc acgcggtgca ccgtggcgtg gtacgtgggc
     3001 aggttggcca ggcccacatg ctcgaacatg cccacgctcg acaccgtgtc gtagacgccc
     3061 tcgccctcga gcgcacggta gtcgcgcagt tcgacggtca cgcggtcctg caggccctcg
     3121 gccgcgatgc gcgcccgggc ctccgcgagt tgctccttgc tcagcgtgat gccgtgcgcg
     3181 tgcacgccat agtgcttggc ggcccagatc accagcgcgc cccagccgca gccgatgtcc
     3241 aggtgccgac cgccgggctt gagccgcagc ttgcggcaga tgtggtcgag cttgttggcc
     3301 tgcgcctggt cgaggctgtc gccgtcctcg tggaaatacg cgcaggagta caccatgcgc
     3361 tcgtcgagga acagcttgta gaagtcgttg gagacgtcgt agtggtgcga gatggccgcg
     3421 cggtcgctgg ccatcgagtg gtcgtgcgtg aactgcgtgg tggccggcgg gtcgccggcg
     3481 ggcgaggccg cgccgcccgg agagaacttc aggcgcagcg cgtcgagcag cagcccgaag
     3541 cgctcgcggc cgctgagcgt caggttctgg aagtggtgct tcaggcccag cgcctcgtac
     3601 aggtcgccct cgacgtcgag gcgtccgcgg aaatacgcgt cggccagggg gatcgggccg
     3661 cgcttgagga cgaggtcgcg cagcacgccg gggtcgcgca ggatcagcgt gaagcgcgac
     3721 gtgccgtgcc cgaagctgtg cgtgacgccg tcccacagcc gcagcgcggc ggccgtggac
     3781 acggagctga gcaggcggcg gacgatgcgg tcggcggctt cggtgatgcc ggggtcgacg
     3841 ccggacgcgg gcagcgtcga ggacgaggtg cgagccatgg gttctccgaa caacagggtt
     3901 tgaagaagga actgcagggc aaggcggacg cccgagcccg gcggcgcggt cacgcgggac
     3961 cgatcgctgg ggggcgccca attccatcct agcgcgcagc gcattttcct gcaggtcctg
     4021 cggccttgcg tgccggccgc gcgtggacac ggtcccgcac ccgtgcgcgt gacgcgcgta
     4081 aatctcgcgg cgccccgcgt caggtcagga cgccgggccc ggcagagccg caccgatcag
     4141 cgccagcagg acgaacacgc cgccgagcac gaggacgacg atgttcatcc agttgaagcg
     4201 cgcgttgcgg tcgtgccccg gtgcgacgac ggcgttgacg cggttcgcgt agatctggac
     4261 gggaatcgtg aggaacggcg ccagcatcga gacccagtcg tacggatcgg gcagccgcca
     4321 gcacagcgag ctcaggatcc aggccacggc caacgggccg aatggcgggc cgccgggcac
     4381 cccggcctcc ttgccggccc gttccacgcg catgaacagc gaccagcaga agatcacccc
     4441 gaagatggcg cggggcacgg gccagatgtc cgagtgatcg cgccgccgga tcgccgccca
     4501 gtgctggtac agccagacca gttcgtacag gccgaaggtg gcgatcgtca gcacgaccat
     4561 cttggcgatc gacacggcga acagcggcgc ctcgggcgcg tcctcgggcc gcgattcgac
     4621 gacgacggcg cgcggcgggg cgtaggggtt ctggtccatg aacccattgt cgatcaactc
     4681 gacagcgggc ggcctcagcg gcgccgcgca gattccacgc cgcccacctt cgccaccagg
     4741 ccaagcacca ctcccagccg ctcggcgctg tcgatctcca ccgtgaacgt catgaacgcg
     4801 cggcggtcgc cgatgccctt ggtcgactgc gaggccacgc cgatcacgtt gacgcgctgc
     4861 ttggccagca cgtcggagat gtcgcgcagc aagccctggc ggtcctgcgc ctcgacgacg
     4921 atgtccacgg cgtagacgtc cttgccggag cggccttccg ccgccgccag cccccattcg
     4981 acggggatga cgcgctcggg attggtctcg gacatgtggc ggaagttcac gcagtcgagc
     5041 cggtgcaccg cgacgccctt gccgcgcgtg acgtagccgc cgatcgcgtc gggcggagcc
     5101 ggccggcagc agctcgcgag cgtcgtcagc agcgagtcga tgcccaccac cagcacgccg
     5161 ccagccgcgc cgccggtgcg cgggcgcttg agctggatcg gctcgtcggg cgcgggcggt
     5221 tcggccgggc gcagcaggtt ctcgatgttg cgcagcgagt attcgtcctt gcccacgacc
     5281 tcgaacagcg cctgcgcgtc gtcgaatccg agcttgccgg cgagctcctc gaggcccgtg
     5341 gcggtgcgac cctcgcgctg aagcagcttt tccaccagct cgcggccctt ggccaccgtc
     5401 tgcgcgtgct gcagcgcgtt gaaccaggcg cgcaccttgg cgcgggcgcg cgggctctgc
     5461 aggaagtgca gctcggggtt gagccagtcc atcgacgggc cgccttcgcg cacggtgacg
     5521 atctccaccg tctgcccgtt ttgcagcggc gtctgcagcg gcaccatcac gccatcgacg
     5581 cgcgcgccgc ggcagcggtg gccgacgtcg gtgtgcagcg cgtaagcgaa gtcgaccggc
     5641 gtgccgccgg cgggcagctc gatcacgctg gcctgcggcg tgaagacgta gatgcgatcc
     5701 tcgaaggcgg gcgcatcgtc accggccgac ggcgacgcct cggcgccggg ctcggcgacg
     5761 aagtcgcgtt cccaggccag cagctggcgc agcaccgcct tgcgcgcctg cgcgacctgg
     5821 tcctcgaacg cgcccgcggc gctgacgccc gcgtagccct tgctgccggc ttccttgtag
     5881 gcccagtgcg cggcgacgcc gtgctcggcg tgctcgtgca tctcgcgggt gcggatctgc
     5941 acctcgaagg ctcgtccctt gtcgtccttg acgacggtgt gcagggactg gtagccgttg
     6001 gccttgggct tggcgatgta gtcgtcgaac tcgccgtcga cggcgcggta gcgctcgttg
     6061 acgcggccca gcgcggcgta gcagtcggcg acgctgtcga cgatcacgcg cacggcgcgg
     6121 atgtcgaaca cgtgctcgaa gtccagctcc ttgccgcgca tcttcttcca gatgctgtag
     6181 atgtgcttgg gccggccctg gatgtcggcg tggatgccgg cgtcggccag catgcgctcg
     6241 agctgcgtgc ggaaggcgac gatgcccgcc tcgcgctcga cgcggcgctc gtccagcagc
     6301 ctggcgacgc gcttgtactc ggccggctcg aggaagcgga acgacaggtc ctccagctcc
     6361 cacttgatct gccagatgcc caggcggttg gccagcggcg cgaacacctg ctgcgactcg
     6421 gccgccagct cgggcgggca tggcgtgcgg ctggacgcgt gccagcgcaa cgtctgcagc
     6481 cgcgaggcca ggcgcagcag caccacgcgc aggtcgcgcg agaacgccag cagcatcttg
     6541 cgcacgcgct cggtctgcag cgcgcgctgt tccttgccga tctgcgcctc gcgcgcgttg
     6601 cgctggatct tgaccagctt gcgcgtgagg tcgaccaggc tcgcgtacga cggcccgaag
     6661 gccttggtca ccacctcgat gggccgctgc aggccgtcgc ccgcgtagac gagccaggcc
     6721 gcggcctgca tcgccggggt ggcgcccagg tcggccagga tctgcgcgac gccctgggcg
     6781 tgcgccaacg cgggctcgcc gctatcgagc agccgcccga ccagcagcgg ctcggcga
//
LOCUS       DATCRO010000848        11576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_628315, whole genome shotgun sequence.
ACCESSION   DATCRO010000848 DATCRO010000000
VERSION     DATCRO010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628315"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VIN75_23045"
     CDS             <1..420
                     /locus_tag="VIN75_23045"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="HEY8977111.1"
                     /translation="VLLENLSAYVECRDSEMTETVFLAELARRTGCGLLLDLNNLYVN
                     ARNFGFDATRRLDALDAASIGQIHLAGHAVVDDCLVDTHGAVVCDPVWSLYAESVRRF
                     GPKPTLIEWDTDLPALDVLLDEAAHARRVMAAEAAHG"
     gene            413..1183
                     /locus_tag="VIN75_23050"
     CDS             413..1183
                     /locus_tag="VIN75_23050"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEY8977112.1"
                     /translation="MADLLAVQAAFAAALRDRARTPAVARWLAGEADVVDRRMAIYRA
                     NMVAAADKALSAACPVIRQVVGGEFFHGLAREYQRGTPSASGDLTGFGATFDTFLATF
                     EHVQDMPWLPDLARLEWAAHVAYGAADAPAWDVAALAAIPPERQDAIRFRWSPGLAVV
                     RSAYPVVRVWTIHQPGFGGAFEVDWQRAETALVARDGFAVMVSDCPPADATFIEASLA
                     GQTLADAASAALDRHPGFDLGALLARAFTARLVCGVID"
     gene            1194..1667
                     /locus_tag="VIN75_23055"
     CDS             1194..1667
                     /locus_tag="VIN75_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8977113.1"
                     /translation="MRQLIQRALVLTRLADHLQPLALLALRLYVSSVFLRAGVIKVSD
                     WSATLALFHDEYKVPLLPPDVAACVGAFGELAFPVLITLGLAGRFGAAGLFVVNAMAV
                     ISYPQLWGFDCPAAIQMHFVWGAILAALVLFGPGRLSLDALVLRRLGLAPRAAAA"
     gene            complement(1676..2035)
                     /locus_tag="VIN75_23060"
     CDS             complement(1676..2035)
                     /locus_tag="VIN75_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977114.1"
                     /translation="MKTILVVDDELGSAEVLSLILEEEGYRALCAVNGRQALIQARDV
                     VPDLVIVDYMMPLMNGAEFSREMRADPQFADTKIILNSGLPESAIREQFDGYDAFLRK
                     PFKVEMLLELVQRFIGR"
     gene            complement(2059..3546)
                     /locus_tag="VIN75_23065"
     CDS             complement(2059..3546)
                     /locus_tag="VIN75_23065"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY8977115.1"
                     /translation="MTSSTTDNVPAIERLRSAVPGLDQILGGGLFRTGVYIIQGLPGC
                     GKTILANQICYGHVAEGGAAVYVTLLAESHSRMIQHLSALSFFDVKAFPDKLAYISAF
                     HELETSGLKGLMALLRREMRTRKIGVLVLDGLVAASEGASDLDLKKFIHELQSIAVLQ
                     DCTVLLLTSGNIHRMAAEHTMVDGLIELEDKLFDARSERSIQVRKFRGAGPLRGKHSM
                     RIDNDGITVFPRIESLYRIAAGDGTGTGHAVTSGVASLDGLLAIRGIPASSATVVIGS
                     TGTGKTTLGLHFLHASTPDDPGLHLGFFEGPDRLRSKARSMGMALEPLEASGAVELMW
                     QSPVEATVDEIAERLLAAVARRGVRRLVIDGLSGFLESTVYPERIARFFSCLVNELRN
                     RHVTVLMTLETRDVVSSVIAMPWGVSGLVDNLFFLRFVHDEGRVERLLTIIKMRDTDY
                     QAGLCRLKIGSQGMHIAGTYRADGDVIPSAQPVASPEGAAPSPSK"
     gene            complement(3543..4304)
                     /locus_tag="VIN75_23070"
     CDS             complement(3543..4304)
                     /locus_tag="VIN75_23070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8977116.1"
                     /translation="MPPDRRRDDASELESLRREIALRDDVLSVAAHELRNPLHALSLH
                     LALARSMAQAGNAVEAAERIRRAELTLKRYAERVTVLMELVATPAATYALDRRRIDVP
                     ALVAALVDSLAEEARSRGIAMRVDCDEAAFGPMRAVDPVALEQVVDNLVLNAFKHSGA
                     SRVTIRLSSGEHWWVLQVEDNGHGIAAADQATIFDKFAVATHSARGAGTGLGLWIVSR
                     LVQSMDGQISVQSVPQQGCAFTVRIPERDDMSTNR"
     gene            complement(4510..6240)
                     /locus_tag="VIN75_23075"
     CDS             complement(4510..6240)
                     /locus_tag="VIN75_23075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY8977117.1"
                     /translation="MTPPIPAALRRSLRDTFGFDELRSGQHDVITRVLAGRPTLAIMP
                     TGAGKSLCYQLPALHLDGATVVVSPLISLMKDQAGKLEEAGVAAEQLNSAVKASEQDA
                     SMERIAEARSDIVFATPERLTDPEFRATLAAQGVALLVVDEAHCISQWGHDFRPAYLE
                     IGAAIEALGHPTVLALTATATAEVVDDIRHQLGVPDMEVVNTGMFRPNLQMQVLQVTS
                     EAEKFDTLRETLRTTPGAGIVYTSTVKTCTEVFERLRDAGESVAIYHGKLPAAERNAN
                     QDAFMSGARRVMVATNAFGMGIDKADIRYVIHFQMPGSMQAYYQEAGRAGRDGRTARC
                     VLIFDRRDRQVQQFFLARRYPLVADLVRVIEALEIAPQGSTQPELVARVHGMPKNRLS
                     VTLSLLRDGGLAQCDATHGWHRTPREATPQLLGELAGDYARKAERDHEALERMVFFAQ
                     TGFCRWKTLLEGFGENLPDGAERCGHCDNCVRFASQAIELLPEPEPVPRLLEVTGFSN
                     SLFEVGEAVKAPKYGEGVVASVKGDQISVRFPDKSLRTFLAEFLKKAKARARRTSTPR
                     RAAARSGAVA"
     gene            complement(6286..7197)
                     /locus_tag="VIN75_23080"
     CDS             complement(6286..7197)
                     /locus_tag="VIN75_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8977118.1"
                     /translation="MYLRRLRHVLSKAGSAWIDDHAQSMGAALSYYTVFSLAPLLLIA
                     ISVAGLVFGREAAQGAVVDQLQGLIGRQGSEAIQDLLKNVSKPSEGVIATITGVVVLV
                     IGATSVFAELQDDLNRIWQVPTPRKVSTWWAWLRTRILSIGLILAMGFLLLVSLAASA
                     AFDAFAAWSTSPIAGWEPLVHAINLVLSFALTTALFALIYRFMPQATIEWRDVGIGAA
                     VTAALFALGKTLIGLYIGKSALASGFGAAGSLAVLLAWVYYSAQIFLFGAEFTWAYAH
                     AFGSRQGRSTETPAAPGDTQRASVRFR"
     gene            7261..7551
                     /locus_tag="VIN75_23085"
     CDS             7261..7551
                     /locus_tag="VIN75_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8977119.1"
                     /translation="MFIVTLTYLKPVEDVDALMPGHVEWLDQGYADGLFLASGRRIPR
                     TGGVILARSGSEAALSDYLARDPFAIHGAARFDVVEFSATKTSAGLEALKGL"
     gene            complement(7538..9763)
                     /locus_tag="VIN75_23090"
     CDS             complement(7538..9763)
                     /locus_tag="VIN75_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8977120.1"
                     /translation="MTAIGRPLDRVDGPLKVCGQAHYTGDIAVARMAHAVLVTSSIAS
                     GRVAAIDTAAAQRAPGVVAVITHLNAMKLPQGGRAAVQPPEGRVLSLLQDDQVAYANQ
                     PVAVVVAETDAQAAGAAALVRVTYERAPVRGDFAAARRDAYPPSQDKMNKMPPDTSRG
                     DIEGGLRSAARRHEAVYTTPFQHHNPLEPHATLARWEGERLEIEDSSQYVAGVRRTLA
                     KTFGIDPSLVHVRSAFVGGAFGGKGSAWSHVVLAAMASRQVGRPVRLALERPQLFGPV
                     GGRPHTWQAIRMGCDADGRLTALEHRSTSTTSTIEDWVETCAVVTRMLYASPAVATTH
                     RLAKMNVGTPTFQRAPGESSGMFALESAMDEMAIAAGLDPIEFRLRNDAKRDDQKDAP
                     FSSKALAECYRIGADKIGWSRRQSIPGALHDGHWLVGLGCASATRPAKRSPCAARVRL
                     TADGRALVGSSTAELGTGSYTVMAQIAADGLGLPVERVRFELGDTDLPEAPISAGSMT
                     VESVGSAIDDACANVRRQLVALAVADHESPLHGLGPEDVTVEDGWLRERAGTGRRDNV
                     AVLLSRRGSAPLVADGSSQPGEEDKQFSMNSFGAVFAEVHVDRDLGIVRVPRIVARFG
                     AGRVINAKTAHSQLLGGIVWGLGMALTEESVLDPRHARFVNANLAEYHVIVNADVGEI
                     DCAFVDEVDPHVNSLGAKGLGEVAMTGVMGAVANAIFNATGRRVRDLPITLDKLLSRV
                     P"
     gene            complement(9760..10746)
                     /locus_tag="VIN75_23095"
     CDS             complement(9760..10746)
                     /locus_tag="VIN75_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8977121.1"
                     /translation="MTPFAYHRARSVEDAVRVGGTPDAHFLGGGTNLVDLAKGGVVQP
                     LLLVDVSRLPLAEVTPLPGGGLRIGAMVRNSDAANHALVRTRHPLLSQALLAGASPQL
                     RNMATVGGNLLQRTRCHYFYDTAFEHCNKRAPGSGCDAVDGVNRNHAILGASPACIAT
                     HPSDMCVALAALRASVRVRGPRGDRSIAMADFHRLPGDTPQRDTSLAPGELILHVDLP
                     PDGFAARSHYLKVRDRASYAFALVSVAAALDVRDGVVHDAAIALGGVAHKPWRRPEAE
                     ARLVGRVPGDDVFGEAADVLLQGARPASQNGFKVTLARRAIVRALHNAGAAS"
     gene            complement(10743..11402)
                     /locus_tag="VIN75_23100"
     CDS             complement(10743..11402)
                     /locus_tag="VIN75_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8977122.1"
                     /translation="MSHDPHAGGATRRGFLQSAGALAGTPLIAGPAPAATHPAAQPQP
                     AAPVALTLRVNGQDHALRLEPRTTLLDALRDHLGLTGTKKGCDRGQCGACTVLVGGRR
                     INACLSLAVQHDGDEIVTIEGLGQPGRLHPLQAAFIAADAFQCGYCTPGQICSGVALM
                     TEARRGDCSAATEDVRRTGALALTDAEIRERMSGNLCRCGAYVNIVDAVRVVARQEGV
                     A"
BASE COUNT         1614 a         4149 c         4109 g         1704 t
ORIGIN      
        1 gtgctgctcg agaatctctc ggcctacgtc gaatgccgcg acagcgagat gaccgagacg
       61 gtcttcctcg ccgaactcgc ccgccgcacc ggttgcgggc tgctgctcga tctcaacaac
      121 ctctacgtca acgccaggaa cttcggcttc gacgcgacgc gacggctcga tgcgctggac
      181 gccgcgagca tcggccagat ccatctcgcc ggccacgccg tggtcgacga ctgcctggtc
      241 gacacgcacg gtgccgtcgt gtgcgacccg gtgtggtcgc tgtacgcgga gtccgtgcgg
      301 cgcttcggtc cgaagccgac gctgatcgaa tgggacaccg acctgccggc cctcgacgtc
      361 ctgctcgacg aagccgcgca cgcccggcgc gtgatggccg cggaggccgc ccatggctga
      421 cctgctcgcc gtgcaggccg cgttcgcggc ggcgctgcgc gatcgtgcgc gcacgcccgc
      481 ggtcgcgcgc tggctcgcgg gcgaggccga cgtcgtcgac cgccggatgg cgatctaccg
      541 ggccaacatg gtggcggcgg ccgacaaggc gctgtccgcc gcatgtccgg tgatccggca
      601 agtggtcggc ggcgagttct tccatggcct ggcgcgcgag taccagcgcg gcacgccgtc
      661 ggccagcggc gacctcaccg gcttcggcgc caccttcgac accttcctcg cgacgttcga
      721 gcacgtgcag gacatgccct ggctgcccga cctggcgcgg ctggagtggg cggcgcacgt
      781 cgcgtacggc gccgccgatg cgcctgcctg ggacgtggcc gcgctcgccg cgatcccgcc
      841 ggaacgccag gacgcgatcc gcttccggtg gtcgccgggg ctcgccgtcg tccggtccgc
      901 gtacccggtc gtgcgcgtct ggacgatcca ccaacccggc ttcggcggcg cgttcgaggt
      961 ggactggcag cgtgccgaaa ccgcgctcgt cgcgcgcgac ggcttcgccg tcatggtcag
     1021 cgactgcccg cccgccgatg ccaccttcat cgaggcctcg ctcgcaggcc agacgctggc
     1081 cgacgccgcg tcggccgcgc tcgaccgcca tcccggcttc gacctcggcg ccctgctggc
     1141 gcgcgccttc acggcgcggc tggtctgcgg cgtcatcgac tgaaaaactc ctcatgcgac
     1201 aactcatcca acgcgccctc gtcctcacgc gactggccga ccacctccaa ccgctggccc
     1261 tgctggcgct gcgcctgtac gtctcgtcgg tcttcttgcg ggccggcgtc atcaaggtct
     1321 ccgactggag tgcgacgctg gcgctgttcc acgacgaata caaggtgccg ctgctgccgc
     1381 ccgacgtcgc ggcgtgcgtc ggcgcattcg gggaactcgc ctttcccgtg ctgatcacgc
     1441 tgggcctggc cggccgcttc ggcgccgcgg ggctgttcgt cgtcaacgcg atggcggtga
     1501 tctcgtatcc gcaactgtgg ggcttcgact gccccgccgc gatccagatg cacttcgtgt
     1561 ggggcgcgat cctcgcggcg ctggtgctgt tcggccccgg ccggctgtcc ctggatgccc
     1621 tggtgctgcg gcgcctgggc ctggcgccgc gggccgcggc cgcctgaggg cgcgatcagc
     1681 ggccgatgaa gcgctgcacg agctccagca gcatctcgac cttgaacggc ttgcgcagga
     1741 acgcgtcata gccgtcgaac tgctcgcgga tcgcggactc gggcaggccg ctgttgagga
     1801 tgatcttcgt gtcggcgaac tgcgggtcgg cgcgcatctc gcgcgagaac tccgccccgt
     1861 tcatcagcgg catcatgtag tcgacgatga ccaggtcggg cacgacgtcg cgggcctgga
     1921 tcagcgcctg gcggccattg acggcgcaca aggcccggta gccttcctct tccaggatca
     1981 ggctcaggac ttccgcactg ccaagttcgt cgtcgacgac aagaatggtc ttcacgcgat
     2041 gcctctatcc ccgcgccgtc atttcgacgg ggaaggcgcg gcgccttccg ggctggcgac
     2101 gggttgggca gaaggaatca cgtcgccgtc tgcccgatag gtcccggcaa tgtgcattcc
     2161 ttgcgatccg atcttcagcc ggcacaggcc cgcctggtag tcggtgtcgc gcatcttgat
     2221 gatcgtgagc agccgttcga cacggccctc gtcatgcacg aagcgcagga agaacaggtt
     2281 gtcgacgagc cccgagacgc cccagggcat ggcgatgacg ctgctgacga cgtcgcgcgt
     2341 ctcgagcgtc atcagcaccg tcacgtggcg gttgcgcaac tcgttgacga ggcaggagaa
     2401 gaagcgcgcg atgcgttccg gatagacggt cgactcgagg aagccggaca ggccgtcgat
     2461 gaccaggcgc cgcacgccgc gccgggcgac ggccgcgagc agccgctcgg cgatctcgtc
     2521 gacggtggcc tccaccggcg attgccacat cagttccacc gcgccagagg cttccagcgg
     2581 ctcgagggcc atgcccatcg agcgcgcctt ggagcgcagg cgatccgggc cttcgaagaa
     2641 ccccaggtgc aggcccggat cgtcgggcgt gctcgcgtgc aggaagtgca ggcccagcgt
     2701 cgtcttgccg gtgccggtgg aaccgatgac gaccgtcgcg ctcgaggccg ggatgccgcg
     2761 gatcgcgagc aacccgtcga gcgacgcgac accactggtg accgcgtgcc ccgtcccggt
     2821 gccgtcgccc gcggcgatgc ggtacagcga ctcgatgcgc gggaagacgg tgatgccgtc
     2881 gttgtcgatg cgcatcgagt gcttgccgcg cagcgggccc gcgccgcgga acttccgcac
     2941 ctggatcgag cgctcggagc gcgcgtcgaa cagcttgtcc tccagctcga tgaggccatc
     3001 gaccatcgtg tgctcggccg ccatgcggtg gatgttgccg ctggtcagca gcagcaccgt
     3061 gcagtcctgc agcacggcga tgctctgcag ctcgtggatg aacttcttga ggtcgaggtc
     3121 gctcgcgcct tccgaggccg cgaccagccc gtcgagcacc agcaccccga tcttgcgcgt
     3181 gcgcatctcg cgccgcagca gggccatcag gcccttcagg ccggacgtct ccagttcgtg
     3241 gaaggcgctg atgtaggcga gcttgtcggg gaaggccttg acgtcgaaga acgacagcgc
     3301 cgacaggtgc tggatcatgc gcgagtgcga ctcggccagc agcgtcacgt agacggccgc
     3361 cccgccctcg gccacgtggc catagcagat ctggttggcc aggatcgtct tgccgcagcc
     3421 cggcaggccc tggatgatgt agacgccggt gcggaacagc ccgccgccca ggatctggtc
     3481 gagccccggg acggcgctgc gcaggcgctc gatggcgggg acgttgtcgg tggtcgagga
     3541 ggtcatcggt tcgtgctcat gtcgtcacgt tcgggaatcc ggacggtgaa ggcgcagcct
     3601 tgctgcggga cgctctgcac cgagatctgg ccgtccatcg actgcacgag ccgggagacg
     3661 atccacaacc ccaggccggt gccggcgccg cgggcggagt gggtcgccac cgcgaacttg
     3721 tcgaagatcg tggcctggtc ggcagcggcg atgccgtggc cattgtcctc gacttgcagc
     3781 acccaccagt gctcgcccga agacaggcgg atggtcacgc ggctggcgcc cgagtgcttg
     3841 aacgcgttga gcaccaggtt gtcgaccacc tgctcgagcg cgaccgggtc caccgcgcgc
     3901 atcgggccga acgccgcctc gtcgcagtcc acccgcatcg cgatgccgcg cgagcgcgcc
     3961 tcctcggcca ggctgtccac cagcgcggcg acgagcgccg gaacgtcgat gcgcctgcgg
     4021 tccagcgcgt aggtggccgc gggcgtcgcc accagttcca tcagcaccgt gacgcgctcg
     4081 gcgtagcgct tgagggtgag ctcggcccgg cggatgcgct ccgcggcctc gacggcgttg
     4141 ccggcctgcg ccatgctgcg cgccagcgcc aggtgcagcg acagcgcgtg cagcgggttg
     4201 cgcaactcgt gggcggcgac cgacagcacg tcgtcgcgca gggcgatctc gcggcgcagc
     4261 gactccagct ccgacgcgtc gtcgcgccgg cggtcggggg gcatggagtg caagagatcg
     4321 tctttcacgg ccgatggcgt catggcgggc gcatcggccg cctccaggaa gagcgaccga
     4381 ctgtacccgg gcaacgcggg actgcggtgc cgctgctcgg caaatatcag gccgggagca
     4441 gatcgttttc catgaggctg aaggccccct gtcgggcacg cccgttgccc tgcgcctgct
     4501 cgggcgcgct caggcgaccg cgcccgatcg cgcggccgcg cgccgcggcg tcgacgtccg
     4561 ccgggcgcgc gccttggcct tcttcaggaa ctccgctaga aaggtacgca gcgacttgtc
     4621 cgggaaacgc accgagatct ggtcgccctt gacgctggcg acgacaccct cgccgtactt
     4681 cggcgccttc acggcctcgc ccacctcgaa cagcgagttc gagaacccgg tgacctccag
     4741 cagccgcggc accggctcgg gctcgggcag cagttcgatc gcctgcgacg cgaagcgcac
     4801 gcagttgtcg caatggccgc aacgctcggc gccgtccggc aggttctcgc cgaagccttc
     4861 gagcagcgtc ttccagcggc agaagcccgt ctgcgcgaag aacaccatgc gctccagcgc
     4921 ctcgtggtcg cgttcggcct tcctggcata gtccccggcg agctcgccca ggagctgggg
     4981 cgtcgcctcg cgcggcgtgc ggtgccagcc gtgcgtggca tcgcactgcg ccaggccgcc
     5041 gtcgcgcagc aggctcaacg tcaccgagag ccggttcttg ggcatgccgt gcacgcgcgc
     5101 cacgagctcg ggctgggtcg atccctgcgg cgcgatctcg agggcctcga tcacgcgcac
     5161 gaggtcggcc acgagcggat agcgccgcgc gaggaagaat tgctgcacct ggcggtcgcg
     5221 gcggtcgaag atcagcacgc agcgcgccgt cctgccgtcg cgcccggcgc ggccggcctc
     5281 ctggtagtag gcctgcatgc tgccgggcat ctggaagtgg atcacgtagc ggatgtcggc
     5341 cttgtcgatg cccatgccga acgcgttggt ggccaccatc acgcggcgcg cgccgctcat
     5401 gaaggcgtcc tggttcgcat tgcgttcggc cgcgggcagc ttgccgtggt agatcgccac
     5461 cgactcgccg gcgtcgcgca ggcgttcgaa cacctccgtg caggtcttga cggtcgacgt
     5521 gtagacgatg cccgcgcccg gcgtcgtgcg cagcgtctcg cgcagcgtgt cgaacttctc
     5581 ggcctcgctg gtgacctgca ggacctgcat ctgcaggttg ggccggaaca tgccggtgtt
     5641 gacgacctcc atgtcgggca cgccgagctg gtggcggatg tcgtcgacga cttcggcggt
     5701 ggccgtggcc gtgagcgcga ggacggtcgg atggcccagc gcctcgatcg cggcgccgat
     5761 ctccaggtag gccggacgga agtcgtggcc ccattgcgag atgcagtgcg cctcgtcgac
     5821 caccagcagc gcgacgccct gggccgcgag cgtggcgcgg aattcggggt cggtcaggcg
     5881 ttcgggcgtg gcgaagacga tgtccgagcg cgcctcggcg atgcgctcca tcgacgcgtc
     5941 ctgctcgctc gccttgaccg cgctgttgag ctgctcggcg gccacgccgg cctcctccag
     6001 cttgccggcc tggtccttca tcagcgagat cagcggcgac acgaccaccg tcgcgccgtc
     6061 caggtgcagc gccggcagct ggtagcacag cgacttgccg gcgccggtgg gcatgatggc
     6121 cagggtgggc cggcccgcga gcacgcgcgt gatcacgtcg tgctggccgg agcgcagctc
     6181 gtcgaagccg aaggtgtcac gcagcgagcg gcgcagcgcc gccggaatgg gaggtgtcat
     6241 gcacgtgatc gaggcaagcg atgtgccact ggcgtgaacg atccttcagc gaaagcgcac
     6301 ggaggcgcgc tgcgtgtcgc cgggggccgc gggggtctcg gtggaccggc cctggcgcga
     6361 cccgaacgca tgcgcatagg cccacgtgaa ctccgccccg aacaggaaga tctgcgcgga
     6421 gtagtacacc caggcgagca gcactgccag cgatcccgcc gcgccgaagc ccgaggccag
     6481 cgcgctcttg ccgatgtaca ggccgatcag cgtcttgccg agcgcgaaca gcgcggcggt
     6541 cacggccgcg ccgatgccga cgtcgcgcca ttcgatcgtg gcttgcggca tgaagcggta
     6601 gatcaatgcg aacagcgcgg tggtcagcgc gaagctcagc acgaggttga tggcgtgcac
     6661 caggggctcc caaccggcga tcggcgacgt cgaccaggcc gcgaacgcgt cgaaggccgc
     6721 gctggccgcc agcgagacga gcagcaggaa gcccatcgcc aggatcaggc cgatcgacag
     6781 gatgcgcgtg cgcagccagg cccaccaggt cgacaccttc ctgggcgtcg gcacctgcca
     6841 gatgcggttc aggtcgtcct gcagctcggc gaagacggag gtcgcgccga tcaccagcac
     6901 caccacgccg gtgatcgtcg cgatcacgcc ttccgacggc ttggacacgt tcttcagcag
     6961 atcctggatc gcctcggagc cctgtcgccc gatcaggccc tgcaattgat cgaccaccgc
     7021 gccctgcgcc gcctcgcgcc cgaacacgag gccggccacc gagatcgcga tcaggagcag
     7081 cggcgccagc gagaacaccg tgtagtacga gagcgccgcg cccatgctct gggcgtggtc
     7141 gtcgatccag gcgctgccgg ccttggacag cacgtggcga agtcggcgca ggtacattgt
     7201 tctcctcgcg gtcgttgccc gacgtcggca acgacgatgc ccgtcccctg cccccacgcc
     7261 atgttcatcg tcacgctcac ctatctgaag cccgtcgagg atgtcgacgc cctcatgccc
     7321 ggccacgtcg aatggctgga ccagggctac gccgacggcc tgttcctcgc atcgggccgg
     7381 cgcatcccgc gcaccggcgg cgtgatcctg gcgcgctcgg gcagcgaggc ggcgctgtcg
     7441 gactaccttg cccgcgaccc gttcgcgatc cacggcgcgg cgcgcttcga cgtggtcgag
     7501 ttcagcgcga cgaaaacgtc cgccggcctg gaagcgctca agggactcta gacagcagct
     7561 tgtccagcgt gatgggcagg tcgcgcacgc gccggccggt cgcgttgaag atcgcgttgg
     7621 cgaccgcgcc catgacgccc gtcatcgcga cctcgcccag gcccttggcg ccgagcgagt
     7681 tcacgtgcgg gtcgacctcg tcgacgaacg cgcagtcgat ctcgccgacg tcggcattga
     7741 cgatcacgtg gtactcggcc aggttcgcat tgacgaagcg ggcatgacgc gggtcgagca
     7801 ccgactcctc ggtcagcgcc atgcccagcc cccacacgat gccgcccagc agctggctgt
     7861 gggcggtctt ggcgttgatg acgcggccgg cgccgaagcg cgcgacgatg cgcggcacgc
     7921 ggacgatgcc caggtcacgg tcgacatgca cctcggcgaa cacggcgccg aacgagttca
     7981 tcgagaactg cttgtcctcc tcgcccggct ggctgctgcc gtcggccacc agcggcgcgc
     8041 tgccgcgccg gctcagcagc accgcgacgt tgtcgcgccg gcccgtgccc gcgcgctcgc
     8101 gcagccagcc gtcctcgacg gtgacgtcct cgggccccag gccgtgcagc ggggactcat
     8161 ggtctgcgac ggccagggcg acgagctggc gccgcacgtt cgcgcaggcg tcgtcgatgg
     8221 ccgagcccac gctctcgacc gtcatcgagc cggcagagat cggcgcctcg ggcaggtcgg
     8281 tgtcgccgag ctcgaagcgc acgcgctcga ccggcaggcc caggccgtcg gccgcgatct
     8341 gcgccatgac ggtgtagctg cccgtgccca gctcggcggt gctggagccc accagcgcgc
     8401 ggccgtcggc ggtcagccgg acgcgggccg cgcaggggct gcgcttggcc ggccgcgtgg
     8461 cgctggcgca gcccaggccg accagccagt gcccgtcatg cagcgcaccc gggatcgact
     8521 ggcggcgcga ccagccgatc ttgtccgcgc cgatccggta gcactcagcc agcgccttgc
     8581 tggagaacgg cgcatccttc tggtcgtcgc gcttcgcgtc gttgcgcagg cggaactcga
     8641 tcgggtcgag cccggcggcg atcgccatct cgtccatggc gctctcgagc gcgaacatgc
     8701 cgctggactc gcccggcgcg cgctggaagg tgggcgtgcc cacgttcatc ttcgccagcc
     8761 ggtgcgtggt cgcgaccgcg gggctcgcat agagcatgcg cgtgacgacg gcgcaggtct
     8821 cgacccagtc ctcgatggtc gaggtggtcg acgtcgaccg gtgctccagc gcggtcagcc
     8881 ggccgtcggc gtcgcagccc atgcggatcg cctgccacgt gtgcggccgg ccgccgacgg
     8941 ggccgaacag ctgcggccgt tccagcgcga gacgcaccgg ccgtccgacc tgccgcgagg
     9001 ccatcgccgc cagcaccacg tgcgaccacg ccgagccctt gccgccgaac gcgccgccga
     9061 cgaaggccga gcgcacgtgc acgagcgacg ggtcgatgcc gaacgtcttg gcgagcgtgc
     9121 ggcggacgcc ggcgacgtac tggctcgagt cctcgatctc cagccgctcg ccttcccagc
     9181 gggccagcgt cgcgtgcggc tccaacgggt tgtggtgctg gaacggcgtc gtgtagacgg
     9241 cctcgtgccg ccgcgccgcc gagcgcaggc cgccttcgat gtcgccgcgg ctcgtgtcgg
     9301 gcggcatctt gttcatcttg tcctgcgacg gcggataggc gtcgcgacgc gcggcggcga
     9361 agtccccgcg cacgggcgca cgctcgtacg tcacgcgcac gagcgcggcc gcgccggcgg
     9421 cctgcgcgtc ggtctcggcc accaccaccg cgaccggctg gttcgcgtag gcgacctggt
     9481 cgtcctgcag cagcgacagc acgcgcccct cgggcggctg gaccgcggcc cggccgcctt
     9541 gcggcagctt catcgcgttc aagtgggtga tgacggcgac caccccgggc gcccgctgcg
     9601 cggcagccgt gtcgatggcc gcgacccggc cgctggcgat gctgctggtg accagcaccg
     9661 cgtgcgccat gcgcgcgacg gcgatgtcgc cggtgtagtg cgcctggccg cataccttca
     9721 gcgggccgtc gacgcgatcc agcggccgtc cgatggccgt cacgacgctg ctcccgcgtt
     9781 atgcagcgcc cggacgatcg cgcggcgcgc cagcgtcacc ttgaacccgt tctgcgaagc
     9841 gggcctggcg ccctgcaaca gcacgtcggc ggcctcgccg aacacgtcgt cacccgggac
     9901 gcggccgacg agccgcgcct cggcctcggg ccgtcgccag ggcttgtggg cgacgccgcc
     9961 gagcgcgatc gccgcgtcgt gcaccacgcc gtcgcgcaca tccagcgccg cggccaccga
    10021 gaccagcgcg aacgcgtagc tcgcgcggtc gcgcaccttg aggtagtgcg agcgcgcggc
    10081 aaagccgtcg ggcggcaggt cgacgtgcag gatcagctcg cccggggcga gcgacgtgtc
    10141 gcgctgcggc gtgtcgcccg gcaggcggtg gaagtcggcc atcgcgatcg agcgatcgcc
    10201 gcggggcccg cgcacgcgca cgctggcgcg cagggccgcg agcgcgacgc acatgtccga
    10261 cggatgcgtc gcgatgcacg ccgggctcgc gcccaggatc gcgtggttgc ggttgacgcc
    10321 atcgacggcg tcgcagccgc tgcccggcgc acgcttgttg cagtgctcga atgccgtgtc
    10381 gtagaagtag tgacagcgcg tgcgctgcaa caggttgccg cccacggtgg ccatgttgcg
    10441 cagctgcggc gacgcgccgg ccaggagcgc ctggctcagc agcggatggc gtgtgcggac
    10501 cagcgcatgg ttggcggcat cgctgttgcg caccatggcg ccgatgcgca ggccgccgcc
    10561 gggcagcggc gtgacctcgg ccaggggcag ccggctcacg tccaccagca gcagcggctg
    10621 cacgacgccg cctttggcca ggtcgacgag gttggtgccg ccgcccagga aatgcgcatc
    10681 gggcgtcccg cccacgcgga cggcgtcctc cacgctgcgg gcgcggtggt atgcgaatgg
    10741 cgtcatgcga cgccctcctg ccgcgccacg acgcgcaccg catcgacgat gttgacgtag
    10801 gccccgcagc ggcacaggtt gccgctcatg cgctcgcgga tctcggcgtc ggtcaacgcg
    10861 agcgcgcccg tgcgtcgcac gtcctccgtc gccgcgctgc aatcgccgcg ccgcgcttcc
    10921 gtcatcagcg ccacgcccga gcagatctgg cccggcgtgc agtagccgca ctggaaggcg
    10981 tccgccgcga tgaacgcggc ctgcagcgga tgcaggcgcc cgggctggcc caggccctcg
    11041 atggtgacga tctcgtcgcc gtcgtgctgc acggccagcg acaggcaggc gttgatgcgc
    11101 cgcccgccga ccagcaccgt gcaggcgccg cactggccgc ggtcgcagcc cttcttcgtg
    11161 ccggtcaagc cgaggtggtc acgcagcgcg tccagcagcg tggtgcgggg ctccaggcgc
    11221 agcgcatggt cctgcccgtt gacgcgcagg gtcagcgcca cgggcgccgc gggctgcggc
    11281 tgcgcggccg gatgcgtcgc cgcaggagcc ggcccggcga tcagcggcgt accggccagg
    11341 gccccggcgg actgcaggaa gccgcggcgc gtcgcgccac cggcatgcgg atcgtgggac
    11401 atggcggact ggcgcgcggc cgacgttgcg accctgccgg ggcggcaatc ggcgtgcgtg
    11461 cccgccgatg gatcaggccg ccgcgtcgcc ctcgtcagcg tggccgttgg tggacgtcgc
    11521 ggacgccgtc ttgcgacggc cgcgcgcgcc gccgaagcgc tcgccgggcg ccgtga
//
LOCUS       DATCRO010000849         8226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_54589, whole genome shotgun sequence.
ACCESSION   DATCRO010000849 DATCRO010000000
VERSION     DATCRO010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54589"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VIN75_23105"
     CDS             complement(<1..361)
                     /locus_tag="VIN75_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8977123.1"
                     /translation="MATAMVAKSAPEHIFEWEGKDRTGKLVRGELRAGGEAAVSASMR
                     RQGIMVTKVRKRRVSGGSSIKQKDIAVFTRQLSTMMKAGVPLLQAFDIVGRGNANPSV
                     AKLLNDIRSDVETGTSLS"
     gene            complement(444..2171)
                     /gene="pilB"
                     /locus_tag="VIN75_23110"
     CDS             complement(444..2171)
                     /gene="pilB"
                     /locus_tag="VIN75_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY8977124.1"
                     /translation="MAVDTFLDAPQTNLAGVARILVRAGRIEPKQAEDLTKTAHDKRI
                     PFVAQLIETGVMKPLALAHALSNALAVPLLDLDAVDSKSLPQGIVDAKLISQYRVYPL
                     GKRGGRLFIACADPTDQEAVERIKFATQLAPEWIVVEYDKLMRLVSDQSASAKETMEA
                     LATSDFEFEVTDEDPNAAPEKQDVATEVEDAPVVKFLHKMLIDAINLRASDLHFEPYE
                     FNYRVRFRIDGELREITQPPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKFGSKS
                     IDFRVSTLPTLYGEKIVIRILDPSSARLGIEALGYEPAEKERLLNAIVRPYGMVLVTG
                     PTGSGKTVSLYTCLNMLNQPGVNISTVEDPAEINMPGVNQVNVNDKAGLNFASALKSF
                     LRQDPDVIMVGEIRDLETADIAIKAAQTGHMVLSTLHTNDAPTTLTRLQNMGVAPFNI
                     ASSILLITAQRLARRLCESCKVPADYPRASLLRAGYTEEELDDTWRPYRAVGCSSCNN
                     GYRGRVGIYQVMPITEKMQQIILAQGNAPDIAACAKADGVRDLRQSGLLKVRLGVTTL
                     EEVISCTNE"
     gene            complement(2477..2553)
                     /locus_tag="VIN75_23115"
     tRNA            complement(2477..2553)
                     /locus_tag="VIN75_23115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2517..2519),aa:Pro,seq:cgg)
     gene            complement(2716..3657)
                     /locus_tag="VIN75_23120"
     CDS             complement(2716..3657)
                     /locus_tag="VIN75_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8977125.1"
                     /translation="MAAEMQAVDDVIRRRLTSEVALVDQIANYIVGAGGKRIRPRLVL
                     LFSRALSFTGDSRFELAAIIEFIHTATLLHDDVVDESALRRGRATANALFGNAASVLV
                     GDFLYSRAFQLMVEIGRMRVLDVLADATNVIAEGEVLQLMNMHDADLAVEDYLRVIRY
                     KTAKLFEASARLGAVIADAPREIEDACANFGRSLGTAFQLVDDLLDYEGDTTALGKNV
                     GDDLREGKPTLPLLYAMSHGTPEEAALIRHAIEQGEVERLAEIVQIVRRTGAIDATRQ
                     AARREADHAREQLALLPDSETKQLLLELCVRSVERSS"
     gene            3989..4300
                     /gene="rplU"
                     /locus_tag="VIN75_23125"
     CDS             3989..4300
                     /gene="rplU"
                     /locus_tag="VIN75_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8977126.1"
                     /translation="MYAVVKTGGKQYKVAAGEKIKVEQIAADVGQEIVLDQVLAVGNG
                     AELTVGAPLVAGASVKATVVAQGRHDKVRIFKMRRRKHYQKHGGHRQHFTELEISAIN
                     A"
     gene            4343..4600
                     /gene="rpmA"
                     /locus_tag="VIN75_23130"
     CDS             4343..4600
                     /gene="rpmA"
                     /locus_tag="VIN75_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8977127.1"
                     /translation="MAQKKGGGSTRNGRDSQPKMLGVKAYGGETVSAGSIIVRQRGTK
                     FHAGENVGMGRDHTLFALVDGKISFATKGSLNRQTVFVTPL"
     gene            4787..5830
                     /gene="obgE"
                     /locus_tag="VIN75_23135"
                     /pseudo
     CDS             4787..5830
                     /gene="obgE"
                     /locus_tag="VIN75_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            6090..7250
                     /gene="proB"
                     /locus_tag="VIN75_23140"
     CDS             6090..7250
                     /gene="proB"
                     /locus_tag="VIN75_23140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY8977128.1"
                     /translation="MNVGAASSAADAAAVNDELRNARRIVVKVGSSLVTNEGRGVDAE
                     AIGNWCRQMAALGQAGREIVMVSSGAIAEGMKRLGWTARPKELHELQAAAAVGQMGLA
                     QMYESKLREHGFGSAQVLLTHADLADRERYLNARSTLVTLLGLKVIPVINENDTVVTD
                     EIKFGDNDTLGALVANLVDADAYVILTDQPGLYTADPRKNPDATFVARARAGDPELER
                     MAGGAGSAIGRGGMLTKILAAKRAAGSGTSTVIAWGRESDVLMRLAAGEPIGTLLVAQ
                     TAKVAARKRWMVDHLQLRGAVVVDDGAAIKLREEGKSLLPIGVREVEGEFHRGDVIAV
                     RALDGRMVARGLANYSSAEARLIARKPSSQIEAALGYANEPELVHRDNLVLG"
     gene            7446..>8226
                     /locus_tag="VIN75_23145"
     CDS             7446..>8226
                     /locus_tag="VIN75_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HEY8977129.1"
                     /translation="MPVVVVGESLNSLGIVRSLAGRGIEVHVAVATRWCPAALSRHAQ
                     VAILPALDGPPLVDGLKALARRLGRRAVLMLCGDRQVDAVNDHRDELAALYQFTLPPA
                     GMLRTLANKATFQQFALAHGLDVPRTAVIQSGQPVAAALRDLVLPLVLKPADKALVLT
                     GHAERAVRVETRAEAEAVAVRMLAGSGCVVAQEWIVGDDSDIYFALFVCDADSHLAGM
                     FCGRKLVCDPPGVGSTGICVDAGEHSATLGREALRFIQASGY"
BASE COUNT         1303 a         2782 c         2843 g         1298 t
ORIGIN      
        1 ccgacagcga ggtgccagtc tcgacgtcgc tgcggatgtc attgagcagc ttggccacgc
       61 tgggattggc gttgccgcgg cccacgatgt cgaaggcctg cagcagcggg acgccggcct
      121 tcatcatcgt cgacagctgg cgcgtgaaga ccgcgatgtc cttctgcttg atggagctgc
      181 cgccgctcac gcgacgcttg cgcaccttcg tgaccatgat gccctggcgg cgcatgctgg
      241 cgctgaccgc cgcttcgccg cccgcgcgca attcgccgcg caccagcttg ccggtgcggt
      301 ccttgccttc ccactcgaag atgtgttccg gggcgctctt cgccaccatc gcagtagcca
      361 tcagtcctcc aaccgccctg tcgggcatgg ttgcgcgatt ctgccttgta ccaggcgtga
      421 tttgagcccg acttgtaagc gactcattcg ttcgtgcagg aaatgacttc ctcgagcgtc
      481 gtcaccccga ggcgcacctt cagcaggccc gactggcgca ggtcgcgcac gccgtccgcc
      541 ttcgcgcagg cggcgatgtc cggggcattg ccctgggcga ggatgatctg ctgcatcttc
      601 tcggtgatgg gcatcacctg gtagatgccg acgcggccgc gataaccgtt gttgcaactg
      661 gagcagccca cggcgcggta cgggcgccac gtgtcgtcca gttcctcctc ggtgtagccg
      721 gcgcgcagca gcgacgcgcg cgggtagtcg gccggcacct tgcagctctc gcacagccgg
      781 cgcgccaggc gctgggcggt gatcagcagg atcgacgagg cgatgttgaa cggcgccacg
      841 cccatgttct gcaggcgggt cagcgtggtc ggggcgtcgt tcgtgtgcag cgtggacagc
      901 accatgtggc cggtctgggc ggccttgatg gcgatgtcgg cggtctccag gtcgcggatc
      961 tcgccgacca tgatgacgtc cggatcctgg cgcaggaacg acttcagcgc cgaggcgaag
     1021 ttcaggccgg ccttgtcgtt gacgttgacc tggttgacgc ccggcatgtt gatttcggcc
     1081 ggatcctcga cggtcgagat attcacgccc ggctggttga gcatgttcag gcaggtatac
     1141 agcgacaccg tcttgccgct acccgtcggg ccggtgacca gcaccatgcc gtacggccgg
     1201 acgatggcgt tgagcagtcg ttccttctcc gccggctcgt agcccagcgc ctcgatgccc
     1261 aggcgcgcgc tggacgggtc gagaatacgg atgacgatct tctcgccata caacgtgggc
     1321 agcgtgctga cccggaagtc gatcgacttg ctgccgaact tgagtttcat ccggccgtcc
     1381 tgcgggacgc gcttctcgga gatgtccagc cgcgagatga ccttgatgcg cgacgccagc
     1441 ttctccttga tggcgatcgg cggctgcgtg atctcgcgca gttcgccgtc gatgcggaag
     1501 cgcacccggt agttgaattc gtagggctcg aagtgcaggt cggacgcgcg caggttgatc
     1561 gcgtcgatca gcatcttgtg caggaacttg acgaccggcg cgtcctcgac ctcggtggcg
     1621 acgtcctgct tttccggcgc cgcgttaggg tcctcgtcgg tgacctcgaa ctcgaagtcc
     1681 gacgtggcca gcgcctccat cgtctccttg gcggaggccg actggtcgct gaccaggcgc
     1741 atcagcttgt cgtactcgac gacgatccac tccggcgcga gctgggtggc gaacttgatg
     1801 cgctcgacgg cctcctggtc ggtcgggtcg gcgcaggcga tgaacagtcg cccgccacgc
     1861 ttgcccagcg gatagacgcg gtactgcgag atcagcttgg cgtcgacgat gccctgcggc
     1921 agcgacttgg agtcgaccgc gtccaggtcg agcagcggca cggccagcgc gttggacagc
     1981 gcgtgcgcca gcgcgagcgg cttcatcacg ccggtctcga tcaactgggc cacgaacggg
     2041 atgcgcttgt cgtgggcggt tttggtcagg tcctcggcct gcttgggctc gatccgaccc
     2101 gcgcgcacca ggatccgcgc gaccccggcg aggttggttt ggggtgcgtc gaggaaagtg
     2161 tcaacagcca tcttcgagga gccgcagcga agtgatgggt gcccaggggc gccgaacggc
     2221 gatcatcgcc caggaataag gtgaagtaaa ttgcgaaaag cctacgataa ctcgcccgtt
     2281 tcgccatggc ggcaaacgcg tgggtactcg tcagttggat tcgtcaggta attggcgaaa
     2341 tccaatgaaa atggattcag gaagtaactc gacagcagaa atgacaaaag gagcccgcgg
     2401 gctccttggt gtcttgcgcc gggccggggc ccgacgcgtc gcatgggccg gacgggccgg
     2461 caccaggcgg atgatctggt cggggtgaga ggattcgaac ctccggcctc tacgtcccga
     2521 acgtagcgct ctaccaggct aagctacacc ccgatgtttc agcgaagctt ggtcagcctc
     2581 tgacgtcgcc cttgcgggcc agccttgccg gctgttcgct cgatccgtcg cgctgcttgc
     2641 ggcgctccgt ttcgctgagc taagcattct cgcacagttc tcgcgggctc gacaagtgcc
     2701 cgcgtgccga tcgcgtcagg acgagcgttc gacggagcgc acgcacagct ccagcagcag
     2761 ctgcttggtc tcggagtcgg gcaggagggc gagctgctcg cgggcgtggt ccgcctcgcg
     2821 gcgggccgcc tggcgggtgg cgtcgatggc gccggtgcgg cgcacgatct gcacgatctc
     2881 ggccaggcgc tcgacctcgc cctgctcgat ggcgtggcgg atcagcgcgg cttcctcggg
     2941 cgtcccgtgg ctcatcgcat acagcagcgg cagcgtcggc ttgccttcgc gcaggtcgtc
     3001 gccgacgttc ttgccgagcg cggtggtgtc gccctcgtag tcgagcaggt cgtcgaccag
     3061 ctggaacgcg gtgcccagcg agcggccgaa gttggcgcag gcgtcctcga tctcgcgcgg
     3121 ggcgtcggcg atgacggcgc ccaggcgggc gctggcctcg aacagcttgg ccgtcttgta
     3181 gcggatgacg cgcaggtagt cctcgacggc caggtcggcg tcgtgcatgt tcatcagctg
     3241 cagcacctcg ccttcggcga tgacgttggt ggcgtcggcc agcacgtcga gcacgcgcat
     3301 gcggccgatc tccaccatca gctggaaggc gcgcgagtac aggaagtcgc ccaccagcac
     3361 gctggcggca ttgccgaaca gggcgttggc ggtggcgcgg ccgcggcgca gcgccgactc
     3421 gtcgacgacg tcgtcgtgca gcagcgtggc cgtgtggatg aactcgatga tcgcggccag
     3481 ctcgaaccgg ctgtcgccgg tgaaggacag ggcgcgcgag aacagcagca ccagccgcgg
     3541 acggatccgc ttgccgccgg cgccgacgat gtagttcgcg atctggtcga cgagcgcgac
     3601 ttcggacgtc agccggcggc ggatgacgtc gtcgacggcc tgcatttcgg cggccatcgg
     3661 gtcgacaggc aggggcgcgt gggtgagggg ggcgttcacg acgacggttc gcgggaaagg
     3721 gaatccggca ttataggtag gctggcggcc tgccgacggg gcgtggcagt ccgctttgac
     3781 gttccgatcg gccgatggta tgatggaggg cttcgccgac ccatggtcgg tcttagcgtg
     3841 tccggaccgg acgccactgc gggcaacaag cggggcgtgc cagtcgggac ggcaagcggg
     3901 aacccccagg ttcccgggcg ccgtccggga ggcgggcaac gcggatctca ccgccgggcg
     3961 gcgagcgaag tcaactgaaa ggtcatatat gtacgcggtc gtaaaaaccg gtggcaagca
     4021 atacaaggtt gctgccggcg aaaagattaa ggtagaacag attgctgcgg acgttggcca
     4081 ggaaatcgtg ttggaccagg ttctcgcagt cggcaacggc gcggaactga cggtcggcgc
     4141 gcccctggtc gccggtgcca gcgtgaaggc taccgtggtt gcgcaaggtc gccacgacaa
     4201 ggtccgcatc ttcaagatgc gccgtcgcaa gcactaccag aagcatggcg gccaccgcca
     4261 gcacttcacc gaactcgaga tctcggccat caacgcgtaa gcgtcgatac gcccgaactc
     4321 attgcacagg agctaatgca ccatggcaca gaaaaaaggc ggcggttcca cgcggaacgg
     4381 ccgtgactcc caaccgaaga tgctcggcgt caaggcctac ggcggcgaaa ccgtctcggc
     4441 cggttcgatc atcgtccgcc agcgcggcac gaagttccac gccggcgaga acgtcggcat
     4501 gggccgcgac cacacgctgt tcgcgctggt cgacggcaag atctcgttcg ccaccaaggg
     4561 cagcctgaac cgccagacgg ttttcgtcac cccgctctga atgagcccca gtcagcggag
     4621 taccgctgcc agcccccaag gggcctagtc cttgcacgtc gggctagcgc agcggcctcg
     4681 agctttcgcc cgctgattga ctggtcatac gtgaacgagc cccggcatgc cggggctttc
     4741 tcgtttattg tcttgaagtc ggcgtcgccg caaggaaagt cctcccatga aattcgttga
     4801 tgaagccgtg atcgacatcg ccgccggcaa cggcggcgcc ggctgcatga gcttccgccg
     4861 ggagaagtgc attcctttcg gcggccccaa cggcggcaac ggcggcaagg gcggcagcgt
     4921 gtggttcgtg gccgaccgca atctcaacac gctgatcgac taccgctaca accgccgcca
     4981 cgaggcgcgc aacggcgagg ccggccgggg ttccgaccag ttcggcgcgg ccgccgacga
     5041 catcgtcctg cgcgtgccgg tgggcacgat gatcaaggac ttcgagaccg accagatcct
     5101 ggccgagctc atcgagccgg acgtccccgt gctgctgtgc aagggcggcg acggcggctt
     5161 cggcaacctg cacttcaaga ccagcaccaa ccgcgccccg cgccagaaga cgccgggctg
     5221 gcccggcgag cagaagaagg tgcggctgga actgcgcgtg ctggccgacg tcggcctgct
     5281 gggcatgccc aacgccggca agtccacgct gatcacggcc atctcgaacg cgcggccgaa
     5341 gatcgccgac tacccgttca ccacgctgca cccgaacctc ggcgtcgtgc gcgtcgcgcc
     5401 cgagaagagc ttcgtcgtgg ccgacattcc cggcctgatc gagggcgccg ccgaaggcgc
     5461 cggcctgggc caccagttcc tgcgccacct gcagcgcaca cgcctgctgc tgcacgtcat
     5521 cgacgcggcg ccggtcagcg acagcgtcga cccggtcaag gaggccaagg cgctggccaa
     5581 ggaactcgag aagtacgacc ccgagctggc cgccaagccg cgctggctcg tgctcaacaa
     5641 gctcgacatg gtgcccgagg aagagcgtgc cgcgcgctgc aaggacatcg tcaggcgact
     5701 gaagtggaag ggcccggtgt tcgagatctc ggcgctcgcg cgcgagggcc tgcagccgat
     5761 gctgcacgcg atctacgagc acgtgcgcgc gcaggtcgag cagccggccc cgccggtcga
     5821 tccgcgcttc gatgcgccgg ccgcgccggc ccccgcggtc gaggcgacgg tgcgccccgc
     5881 gcgcgccgcc cgcaaggtgg ccaaggtcgc caagtcgacc caggccgaca acgacgagcc
     5941 ggtcgatccg cgcttcgcgc cgatcgatcc cgaggccgac gaggcgcccg cgcccgccgc
     6001 tcccgttcgt cgacgcaccc gcgcagcatg agcgccgccc ggccgcccca aggcgctcgc
     6061 tccgcggtcc gcagcacgga ggttgcccca tgaatgtcgg cgcagcgtcc tccgcggccg
     6121 acgccgccgc ggtcaacgac gagctgcgca acgcgcgccg catcgtcgtc aaggtcggct
     6181 cgagcctggt caccaacgag ggccgcggcg tcgacgccga ggcgatcggc aactggtgcc
     6241 gccagatggc cgcgctcggc caggccggtc gcgagatcgt gatggtgtcc tcgggcgcca
     6301 tcgccgaggg catgaagcgc ctgggctgga cggcgcgccc caaggagctg cacgagctgc
     6361 aggccgccgc cgccgtgggc cagatgggcc tggcgcagat gtacgagagc aagctgcgcg
     6421 agcacggctt cggcagcgcg caggtgctgc tcacgcacgc cgacctggcc gaccgcgagc
     6481 gctacctcaa cgcgcgctcc acgctggtca cgctgctcgg gctcaaggtg atcccggtca
     6541 tcaacgagaa cgacacggtc gtcaccgacg agatcaagtt cggcgacaac gacacgctcg
     6601 gcgcgctggt ggccaacctc gtcgacgccg acgcctacgt catcctcacc gaccagcccg
     6661 gcctgtacac cgccgacccg cgcaagaacc ccgacgcgac gttcgtcgcg cgcgcacgcg
     6721 cgggcgatcc cgagctggaa cgcatggccg gcggcgccgg ctcggccatc ggccgcggcg
     6781 gcatgctcac caagatcctc gcggccaagc gcgccgcggg ctcgggtacc agcaccgtca
     6841 tcgcctgggg gcgcgagtcc gacgtgctga tgcgcctggc cgccggcgag cccatcggca
     6901 cgctgctggt cgcccagacc gccaaggtcg cggcgcgcaa gcgctggatg gtcgatcacc
     6961 tgcagctgcg cggcgcggtg gtggtcgacg acggcgcggc gatcaagctg cgcgaggaag
     7021 gcaagagcct gctgccgatc ggcgtgcgcg aagtcgaggg cgagttccat cgcggcgacg
     7081 tcatcgccgt gcgcgcgctc gacggccgca tggtggcgcg cggcctggcg aactattcgt
     7141 cggccgaggc gcgcctgatc gcgcgcaagc cgtcctcgca gatcgaggcg gcgctcggtt
     7201 atgccaacga gcccgagctg gtgcaccgtg acaaccttgt cttgggataa gccggtcgat
     7261 cgacgctcga atcccctgtc ggacgctccc ctaatccggt gacctgacag gccggcttca
     7321 ggcgcgccgc gcactgtcga ggaacattct cgactgtgca cggttcactc gcggctcccg
     7381 cgtctgccga ggcaacgtgg gaagtcaggg ccatcatggc tgcgggtcga acagaacaac
     7441 agagggtgcc ggtcgtcgtc gttggcgaga gtctcaacag cctgggcatc gtgcgcagcc
     7501 tggccggccg cggcatcgag gtccacgtcg ccgtggccac gcgctggtgc ccggcggccc
     7561 tgtcgcgcca cgcgcaggtc gccatcctgc cggcgctcga cggccctccg ctcgtcgacg
     7621 gcctcaaggc cctcgcgcgc cgcctcggcc gccgcgccgt gctgatgctg tgcggcgacc
     7681 gccaggtcga cgccgtcaac gatcatcgcg acgagctcgc ggcgctctac cagttcacgc
     7741 tcccgccggc cgggatgctg cgcacgctgg ccaacaaggc gacgttccag cagttcgcgc
     7801 tggcgcatgg cctggacgtg ccacgcacgg ccgtcatcca gtccggccag ccggtggccg
     7861 cggcgctgcg cgacctggtg ctgccgctgg tgctcaagcc cgccgacaag gcgctcgtcc
     7921 tcaccggcca cgcggagcgc gccgtgcgcg tggagacgcg ggccgaggcg gaggccgtcg
     7981 ccgtgcgcat gctcgccggc tcgggctgcg tcgtcgcgca ggaatggatc gtcggcgacg
     8041 actcggacat ctacttcgcg ctgttcgtct gcgacgccga cagccacctg gccggcatgt
     8101 tctgcggccg caagctggtg tgcgacccgc cgggcgtcgg cagcacgggc atctgcgtcg
     8161 acgccggcga gcactcggcc acgctgggcc gcgaggcgct gcgcttcatc caggcgagcg
     8221 ggtacc
//
LOCUS       DATCRO010000850         6677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1021833, whole genome shotgun sequence.
ACCESSION   DATCRO010000850 DATCRO010000000
VERSION     DATCRO010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021833"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VIN75_23150"
     CDS             <1..1145
                     /locus_tag="VIN75_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8977130.1"
                     /translation="LYVRQLPGAGDTPAFLPPAVVAAASGPGVRQVRAERLLPVTLAP
                     DQPEAALLIRDLLPGETQPHLPWIVEPDDAAPAPNDPLSVYPTEVLAALQHLKKGDVV
                     WLPLPPPRGAGASSTSRTTSVRAVVRGVWRDYARQQGALLMSRADWLRLTGDDRVSGL
                     AVWLADSGGQDDSNTAARADAGSGAHARGGASANAHSGAEAQARAGAATRDAVVARIK
                     AAVPPDAPAEIATASELRAYSLRIFDRSFAVTRWLQGVALAIGLAGIAASFSAQVLAR
                     RREFGTLQHLGFTRRQVLALVASEGALWSAVGAVLGLALGLAVSVVLVKVVNPQSFHW
                     TMQMSVPPGDLLVLGLGVVLAGAFTAWGSGHAAAGRDVVRAVKEDW"
     gene            complement(1695..2342)
                     /locus_tag="VIN75_23155"
                     /pseudo
     CDS             complement(1695..2342)
                     /locus_tag="VIN75_23155"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
     gene            2639..3676
                     /locus_tag="VIN75_23160"
     CDS             2639..3676
                     /locus_tag="VIN75_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY8977131.1"
                     /translation="MSHRPLVFPADHGGHPDTHVEWWYVTGWLRSGPDERAAETPEFG
                     FQVTFFRTRTGLAADSASRFAARQLVFAHVALTDLTSAARHDQRVAREGFGIAQTPGP
                     SLPQTVRLHDWSLTRRAPSPPGDTRSRLRVDMRADRFALALDLTGTQPLLLQGEAGWS
                     QKAPDRAVASHYYSEPQLAVRGRIERATAPARDVSGRAWLDHEWSDQYLAHGAVGWDW
                     VGFNLIDGSALMAFRLRHADGSTIWAGGSFRSAGGARSNFAAGDVRFEPLRRWTSPRT
                     QASWPVEWALATPAGRWRVAALQDDQELDSRGSTGAIYWEGLSALRGDDGRAVGWGYL
                     ELTGYAGQLRL"
     gene            3780..4025
                     /locus_tag="VIN75_23165"
     CDS             3780..4025
                     /locus_tag="VIN75_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977132.1"
                     /translation="MKAQNDSTDPQHIYRQLKLGVAHELVNDQNVFDLIALAERDGNT
                     LLATELREWQAPCSDSMDGPRPTMVPTRGFNKDNAKH"
     gene            4029..4775
                     /locus_tag="VIN75_23170"
     CDS             4029..4775
                     /locus_tag="VIN75_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY8977133.1"
                     /translation="MTTRSALPAAAGKTRPAPARPARAAVAGPASARGPAVVARTAAD
                     EAASTARRPIALATLRAQASHCRECPLGALATQTVFGEGAGHARLMIVGEQPGDREDL
                     VGRPFVGPAGHLLDKALAALGWDREAVYLTNAVKHFKHELRGKRRMHKTPGQLEILAC
                     AHWLQGEIEAVGPAALVALGGTAARSLMGRPVKVLSERGRWLQRSDGRRVLVTLHPSA
                     LLRGDPAGREREFERWVHDLALASDVLRKH"
     gene            4906..5265
                     /locus_tag="VIN75_23175"
     CDS             4906..5265
                     /locus_tag="VIN75_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY8977134.1"
                     /translation="MSDSDELGKLGDLHQRGILSDEEFARAKARVLSGSPSARSTPAY
                     SPLHQFRRSLSDRWLGGVCGGIGQVTGVPSWVWRLVFTFGIVFAGCGLALYILLWILV
                     PQEQLYLPAPGDSARAN"
     gene            5350..5757
                     /locus_tag="VIN75_23180"
     CDS             5350..5757
                     /locus_tag="VIN75_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977135.1"
                     /translation="MIHIRDIDHIVLRVVDLPSMLRFYCEGLGCHIERRQDEIGLVQL
                     RAGRSMLDLVPVDGKLGRAGGAAPGKEGHNVDHMCFRVEPFDEAAIRAHLASLGIEAG
                     PIASRYGAEGEGPSIYLSDPQGNVVELKGPPDA"
     gene            5773..5901
                     /locus_tag="VIN75_23185"
     CDS             5773..5901
                     /locus_tag="VIN75_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977136.1"
                     /translation="MTPERRHATHDDLDALHAQLNTAKDKTGHVARPAAFARLAQR"
     gene            6130..>6677
                     /locus_tag="VIN75_23190"
     CDS             6130..>6677
                     /locus_tag="VIN75_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977137.1"
                     /translation="MATTPPRRSSFALRALASSALLALAAVASGPAAAAGAGDTYTLT
                     LTYDPHNLVAPPAVGSFVLGTEVAGHPGVFAVSDFSIVIGVTPDAWTYDQLHAELDFD
                     TTTGQFGGAGTAAHAFTTYGDQLDLLSGTNDWKTDDFVDFHCTDSGCPDFHYGTYAAS
                     LAAVPEPGGIALGLAGLGALAL"
BASE COUNT          912 a         2538 c         2376 g          851 t
ORIGIN      
        1 acctctatgt gcgccagctg cccggcgccg gcgacacccc ggcgttcctg ccgccggcgg
       61 tggtcgcggc cgcgtcgggc cccggggtgc gccaggtgcg agccgagcgc ctgctgccgg
      121 tcacgctggc gcccgaccag cccgaggccg cgctgctgat acgcgacctg ctgcccggcg
      181 agacgcagcc tcacctgccg tggatcgtcg aaccggacga cgcggcgccg gcgccgaacg
      241 acccgctgtc ggtctatccc accgaggtgc tggccgcgtt gcagcacctg aagaagggcg
      301 acgtggtctg gctccccttg ccgccgcctc gcggcgcggg cgcgagctcg acgtcgcgga
      361 cgaccagcgt gcgcgccgtc gtccgcggcg tctggcgcga ctacgcccgc cagcagggcg
      421 cgctcctgat gtcacgcgcc gactggctgc ggttgaccgg cgacgaccgc gtcagcggcc
      481 tggcggtctg gctcgcggac tccggcggtc aggacgattc caacaccgcc gcacgcgcgg
      541 acgccggcag cggggcgcac gcgcgcgggg gcgcgagcgc gaacgcgcac tcgggcgcgg
      601 aagcgcaggc gcgcgcaggc gccgccacgc gcgacgccgt cgtcgcgcgc atcaaggccg
      661 ccgtcccgcc cgatgcgccc gccgagatcg cgaccgccag cgagctgcgc gcctactcgc
      721 tgcgcatctt cgaccgcagc ttcgccgtca cgcgctggct ccagggcgtg gcgctggcga
      781 tcgggctggc cggcatcgcg gcgagcttct ccgcgcaggt gctcgcgcgc cggcgcgagt
      841 tcggcacgtt gcagcacctc gggttcacac ggcgccaggt gctggcgttg gtggcgtccg
      901 agggcgcgtt gtggagcgcg gtcggcgcgg tgctgggcct ggcgctgggc ctggccgtga
      961 gcgtcgtgct ggtgaaggtg gtcaacccgc agagtttcca ctggacgatg cagatgagcg
     1021 tgccgcccgg cgacctgctg gtgctgggac tgggcgtcgt gctggccggt gcgttcaccg
     1081 cctggggcag cggccacgcc gcggcggggc gcgacgtggt gcgcgcggtc aaggaggact
     1141 ggtagcgacg gggacgcgcc cggcccgcat cagcgctccc cgctcgtcgc tcgatcgctg
     1201 gcccgggttg acggggagcc gccgcgccgc ttcaaggctg gctcgaacac gtccatcgtc
     1261 gacccgatgc gatagtcgta cagccccggc ggcagcgcgc tgctgcggaa ctggccgagc
     1321 gcctccgcgc gcgtgccggg aaaggccgac ggcacccatt cgcgcccgcg ctcgagcacc
     1381 gccaggcgcc gcccgggcgc gagccgcgcg gccatcaccg caccgccgtc acccgagccg
     1441 atcacgacga cgtcgcagct cgcctgcatc tgcgcgaccg ggcgcgacag cggtgcgcgc
     1501 gcgatcgagg cgacccgcgt gcgcgtcagg gcctgcgcgg gcggcacgcc cgccgcggtg
     1561 aggacgccga gcgcggcgac gaagcgccgg cggccggagg ctggaggctg gatggggcat
     1621 cgcatggctc ccgtcgaatc gatcgtcggg tcggccggcg cggccgcggc acccgtgtcc
     1681 agcgtgcctg cgagccggtc gcacagcggg aacacgaggt tgaagttggc ttgctgcatg
     1741 cgccgcggat cgtggtgcac gcggtgcagg cgcgcgagcc agcgcacgcc cggaaggcgc
     1801 gccacgcgcc agtgcgcggg cacgtggtac aggaaatgca gcacctcgta gagcaggtag
     1861 tagccgagcg cgctcgccac gaacagcgcc gcgacgccgg tgggcgcgac gagcgcgacc
     1921 agcgccccga gcccgcacgc gatggcgccg aagaaggcga gcagcaccgg cgggaacagc
     1981 acggcatgga aatcgcgcgc gctctcgaag ggcatggccg cgtcgacgaa gaatcgatgg
     2041 tggcgccgcg aatggcgcgt gtgcagcgcg gcgagcgcct tcgtgcggtg atgcatcggt
     2101 ccgcgatgcg cgaggtattc gaccgcgttg gcgacgacga aggtcagcgg cacgatccag
     2161 ccatcggccc agcgcgcacc gtgcgccagg gcgaacgcga gggctgccgc gatgccgccg
     2221 cccgcggcca tgagcaccag cgtgacatgc gccgcgcccg agtagcgcgg cggcagcacg
     2281 tcggcgcggt attcggcgcg gaacgccgcg gtcttggacg ggacggatcg gggcgaggcc
     2341 atgggggcgc atcattggcg ggcagatcgc gccatgtctt gtcctgatgc gacacgcagg
     2401 ccgcgcgcgg cgccatctcc tggaaaatcc tcatgctggt tttccaggag acgcccgcgt
     2461 tcgacgcgcc gccgaccgaa gcccgaactc gccgacactg ccgcatgccc tcgcctcgtc
     2521 ccgcccctcg ccggagctcc cggtgatgcg ccgccgcccc ttcctcggac tgctgccggc
     2581 caccctcgcc tcgacggggg ctgcgcgggc cgccgccttg gagcacccgc ccctcggcgt
     2641 gtcacaccgc cccctcgtct tcccggccga ccacggcggc cacccggaca cccacgtcga
     2701 gtggtggtac gtcaccggct ggctgcgctc gggcccggac gaacgcgccg ccgagacgcc
     2761 cgagttcggc ttccaggtca cgttcttccg cacgcgcacc ggcctggccg ccgactcggc
     2821 cagccgcttc gccgcgcgcc agctcgtgtt cgcgcacgtc gcgctcaccg acctgacgtc
     2881 ggccgcccgg cacgaccaac gcgtcgcccg cgagggcttc ggcatcgccc agacgcccgg
     2941 gccgtccctg ccgcagaccg tgcggctgca cgactggtca ctgacgcgcc gggcaccgtc
     3001 gccccccggc gacacgcgca gccgcctgcg cgtggacatg cgcgccgacc gcttcgcgct
     3061 cgcgctcgac ctcaccggca cgcagcccct gctgttgcag ggcgaggccg gctggtcgca
     3121 gaaggcgccc gatcgcgccg tggccagcca ctactacagc gagccgcagc tcgccgtgcg
     3181 tggccgcatc gagcgcgcca ccgcgcccgc gcgggacgtg agcgggcgcg cgtggctaga
     3241 ccacgaatgg agcgaccagt acctggcgca cggcgccgtc ggttgggact gggtcggctt
     3301 caacctgatc gacgggtcgg cgctgatggc attccgcctg cgccatgccg acggcagcac
     3361 gatctgggca ggcggctcgt tccgcagcgc gggcggcgcg cgaagcaact tcgccgccgg
     3421 cgacgtccgc ttcgagccgc tgcgccgctg gacgagcccg cgcacccagg ccagctggcc
     3481 ggtcgagtgg gcgctggcga cgcccgcggg gcgctggcgc gtcgcggccc tgcaggacga
     3541 ccaggagctc gacagccgcg gctccacggg cgcgatctac tgggagggcc tgtccgcgct
     3601 gcgcggcgac gacggccgcg ccgtgggctg gggctacctt gagctgaccg gctacgccgg
     3661 gcaattgcgc ctgtagcgac caaggtctat cgcgtgcgga gcacactgcg cacacggcac
     3721 gtccccggca ccggacgggc acaggcgttg ccgcgaagcg attcattcct ggagagccca
     3781 tgaaagcgca gaacgactcg accgatccgc aacacatcta ccgccagctc aagctcggcg
     3841 tcgcgcacga gctggtcaac gaccagaacg tcttcgactt gatcgccctg gcggaacgcg
     3901 atggcaacac gttgctggcg accgagctgc gcgaatggca ggccccctgc agcgactcca
     3961 tggacggtcc gcgtccgacc atggtgccca cgcgcggctt caacaaggac aacgccaagc
     4021 actgagccgt gaccacccga tccgcccttc ccgccgcggc cgggaagacg cgccccgcgc
     4081 cggcacggcc cgcccgtgca gcggtcgcgg gcccggcgtc cgcccgcggc cccgccgtcg
     4141 tcgcccgcac cgccgccgac gaagccgcgt cgacggcgcg ccgtcccatc gcgctcgcga
     4201 ccttgcgcgc gcaggcgtcc cactgccgcg aatgcccgct cggcgccctg gcgacgcaga
     4261 cggtcttcgg cgaaggcgcg ggccacgcgc ggctcatgat cgtcggcgag cagcccggcg
     4321 atcgcgagga cctcgtgggc cggccgttcg tcggcccggc cggccatctg ctggacaagg
     4381 cgctggccgc cctgggctgg gatcgcgagg cggtctacct gaccaacgcc gtcaagcact
     4441 tcaagcacga actgcgcggc aagcgccgca tgcacaagac gccggggcag ctggagatcc
     4501 tcgcctgcgc gcactggctg cagggcgaga tcgaggcggt cgggccggcg gcgctggtcg
     4561 ccctcggggg cacggccgcg cgttcgctga tgggtcgtcc ggtgaaggtg ctgtccgagc
     4621 gcggccgatg gctgcagcga agcgacggcc ggcgcgtgct cgtcacgctg cacccttcgg
     4681 cactgctgcg cggcgatccg gccggacgcg agcgcgagtt cgagcgctgg gtgcacgatc
     4741 tcgcgctcgc cagcgacgtg ctgcgcaagc attgagcgcc gctccatcgg ccgcccgggc
     4801 ggccctgtcg gcctgcgctg cagcccgtcg cacgcggctg gcgacaccgg ggacgcgggc
     4861 gtaacctgca gtcatcgaat caacgcttcc accaaggaga acaccatgtc tgacagcgac
     4921 gaactcggca agctcggcga cctccaccag cgcggcatcc tgtccgacga ggagttcgcg
     4981 cgcgccaagg cccgcgtgct cagcggctcg ccctccgcgc gcagcacgcc cgcgtattcg
     5041 cccctgcacc agttccgccg cagcctcagc gaccgctggc tcggcggcgt gtgcggcggc
     5101 atcggccagg tcaccggcgt gccgtcgtgg gtgtggcgcc tcgtgttcac gttcggcatc
     5161 gtcttcgccg gctgcggcct cgcgctgtac atcctgctgt ggatcctcgt gccgcaggaa
     5221 cagctgtacc tgccggcgcc gggcgactcg gcacgcgcca actgacggct tgtccccgcg
     5281 atgcgcgcgg gtcacgcgcg cgcatcgcgt gggacactcg tgaatcgacc cacaagttgc
     5341 gcttcaccaa tgatccacat ccgcgacatc gaccacatcg tcctgcgcgt ggtcgacctg
     5401 ccctcgatgc tgcgcttcta ctgcgagggc ctgggctgcc acatcgagcg ccggcaggac
     5461 gagatcggcc tggtccagct gcgcgcgggc cgctccatgc tcgacctcgt gcccgtggac
     5521 ggcaagctcg gacgcgcggg cggcgccgcg ccgggcaagg aaggccacaa cgtcgaccac
     5581 atgtgctttc gcgtcgagcc cttcgacgaa gccgccatcc gcgcgcacct cgcgtcgctg
     5641 gggatcgagg ccggaccgat cgcgtcgcgc tacggcgccg agggcgaagg gccgtcgatc
     5701 tacctgagcg atccgcaggg caacgtcgtc gagctcaagg ggccgcccga cgcctgagcg
     5761 cgacgccgcc ccatgacgcc cgaacgccga cacgccaccc acgacgacct cgacgcgctg
     5821 catgcccagc tgaacacggc caaggacaag acgggccacg tcgcacgtcc cgccgcgttc
     5881 gcgaggttgg cgcagcgctg agcgcccgac cgagcggaca cgtcgaacga gggatgccgc
     5941 gcgggtgaac aagccaccga tcgcggcagc gagcgatggt cgatgcgtga agcccgcctt
     6001 gaatgcggga tgggcccgcg ccgccgcggc cgttaccgtg ccgacgctgc gacgtcgcac
     6061 gacgtcccgg ccgcaggtgg cgcgctccgc gacgccggcg cgcatccatc catccccgga
     6121 gatccgtcca tggccacgac gcccccgcgc cgctcgtcct tcgcgcttcg cgcgctcgcc
     6181 tcgtccgcgc tgctcgcgct cgccgctgtc gccagcggcc ccgccgccgc ggccggcgcc
     6241 ggcgacacct acacgctgac gctcacgtac gacccgcaca acctggtcgc gccgcccgcc
     6301 gtcggcagct tcgtcctcgg caccgaggtc gcgggccacc ccggcgtctt cgcggtgagc
     6361 gacttcagca tcgtgatcgg cgtcacgccg gatgcgtgga cctacgacca gttgcacgcg
     6421 gagctggact tcgacaccac caccggccag ttcggcggcg cgggcaccgc cgcccatgcg
     6481 ttcacgacct atggcgacca gctcgacctg ctctcgggca ccaacgactg gaagaccgac
     6541 gacttcgtcg atttccactg caccgattcc ggctgccccg atttccacta cggcacctac
     6601 gccgccagcc tggccgccgt gcccgaaccg ggcggcatcg cgctcggctt ggcgggactg
     6661 ggcgcgctgg cgctgat
//
LOCUS       DATCRO010000851         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_256668, whole genome shotgun sequence.
ACCESSION   DATCRO010000851 DATCRO010000000
VERSION     DATCRO010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256668"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..993
                     /locus_tag="VIN75_23195"
     CDS             61..993
                     /locus_tag="VIN75_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEY8977138.1"
                     /translation="MSRPGPADDLSQLSLFDAPPSSGTPSAPPRSRDRPIAQAQADAP
                     DDTPPPTRRDDPSPLHFRHPQAERELQLGEHRVAYALRRARRSSIGFVVSAEGLQVNA
                     PRWVSLQNIDAAVRSKSGWILRKLQEQRERARRVEAARIEWRDGTTLPFLGETVIIVL
                     DPRVAGAVLNADAQSLPGVPRLVLHVGLPQTAEPEQIRDIVQSWLQRQALRIFDDRCK
                     HFAQTLGVRMTRLSLSSAQTRWGSASADGSIRLNWRLVHFGLQVIDYVVAHELAHLRE
                     MNHSAAFWDVVRSVVPDYEKMRNTLKDVPVPVLG"
     gene            complement(1238..2299)
                     /locus_tag="VIN75_23200"
     CDS             complement(1238..2299)
                     /locus_tag="VIN75_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977139.1"
                     /translation="MANADESKLAYPLGDTLPATGAVLDVAPGVRWLRMPLPFALDHI
                     NLWLLRDRADDGREGWAIVDCGVANEATRGAWEQVFAHQLDGLPVLRVIVTHMHPDHI
                     GNAHWLTERWGCRLWISATDWNAARLASQSTTGFGGDDAAAFFGEHGLVDPEAVAKVR
                     GRGGYYASMVPQVPRRFRRLMEGLVIRIGDHDWRCIAGYGHAPEHISLHCAAFGVLIS
                     GDMLLPRISTNISVIDIEPEANPLPLFLASIERLRALPADTLVLPSHGKPFIGAHERV
                     DQLVAHHEDHFEAVVKACRARPCSAADLLPVLFRRQLDLHQTTFAMGEAVAHLHALAG
                     MGRLRGRLDADGVRRFSAT"
     gene            2349..>2541
                     /locus_tag="VIN75_23205"
     CDS             2349..>2541
                     /locus_tag="VIN75_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8977140.1"
                     /translation="MSASPSTAGRNKAGKAPAAAKGAAKSFTITELAAEFDVTPRAIR
                     FYEDVGLLEPTRAGRNRVYS"
BASE COUNT          395 a          897 c          889 g          360 t
ORIGIN      
        1 cgccggggtc gcgccctttc gcgcacgaac ttccgggaca gcgcactaga atcgatgccc
       61 atgtcccgac ccggcccggc cgacgacctc tcgcagctct cgctcttcga cgccccgccg
      121 tcgagcggga cgccgtcggc gccgccgcga tcgcgggacc gcccgatcgc gcaggcccag
      181 gccgacgcgc ccgacgatac cccgcccccc acgcgccgcg acgacccgtc gccgctgcat
      241 ttccgccatc cgcaggccga gcgcgagctg cagctgggcg agcatcgcgt ggcgtacgcg
      301 ctgcgccgcg cccgccgcag cagcatcggc ttcgtcgtca gcgcggaagg gctgcaggtc
      361 aacgccccgc gctgggtgtc gctgcagaac atcgacgccg cggtgcgttc caagtccggc
      421 tggatcctgc gcaagctgca ggagcagcgc gagcgcgcgc gccgcgtcga ggccgcgcgc
      481 atcgagtggc gcgacggcac cacgctgccg ttcctcggcg agacggtcat catcgtgctc
      541 gacccgcgcg tggccggcgc ggtgctcaac gccgacgcgc aatcgctgcc cggcgtgccc
      601 cgcctcgtgc tgcacgtggg cttgccgcag acggccgagc ccgagcagat ccgcgacatc
      661 gtccagagct ggctgcagcg ccaggcgctg cgcatcttcg acgaccgctg caagcacttc
      721 gcgcagacgc tgggggtgcg catgacgcgg ctgtcgctca gctccgcgca gacgcgctgg
      781 ggttcggcgt cggccgacgg ctcgatccgc ctgaactggc gcctcgtgca cttcgggctg
      841 caggtgatcg actacgtcgt cgcccatgaa ctcgcgcatc ttcgcgagat gaaccacagc
      901 gccgcgttct gggacgtggt gcgttccgtg gtgcccgatt acgagaagat gcgcaacacg
      961 ctcaaggacg taccggtacc cgtattgggc tgaccgaccg taaagcgtgc gtgaagcttc
     1021 agctaactgg gaaaagaagc ccaaacaccc ccaaaagggg gatggcgact gccgagtctg
     1081 agggcacatt agtacctgca tgctgtcatc acccgacagg gacaggggcg catcagggag
     1141 tgaaagccgg acataaccgg cagcagggcc gatcgtttcg cgacgatcgg ctttgttttt
     1201 tccggcgccc gttttccggg gcggcgacag ttcccgctca ggttgcggag aagcgccgca
     1261 cgccgtccgc gtcgagcctg ccgcgcaacc ggcccatacc ggcgagggca tgcaggtgcg
     1321 cgacggcctc gcccatcgcg aaggtggtct ggtgcaggtc gagctggcgg cggaacagca
     1381 ccggcagcag gtcggccgcg ctgcagggcc tggcgcggca ggccttgacc acggcctcga
     1441 aatggtcctc gtggtgggcg acgagctggt ccacgcgttc atgcgcgccg atgaacggct
     1501 tgccgtgcga cggcagcacc agcgtgtccg cgggcagcgc gcgcaggcgc tcgatcgacg
     1561 cgaggaacag cggcagcggg ttggcctccg gctcgatgtc gatcacgctg atgttggtcg
     1621 agatgcgcgg cagcagcatg tcgcccgaga tcagcacgcc gaacgcggcg cagtgcagcg
     1681 agatgtgctc cggcgcgtgg ccgtagccgg cgatgcagcg ccaatcgtgg tcgccgatgc
     1741 ggatgaccag gccttccatc agccggcgga accggcgcgg cacctgcggc accatcgaag
     1801 cgtagtagcc gccgcgcccg cgcaccttgg cgacggcctc ggggtcgacc aggccgtgct
     1861 cgccgaagaa cgcggccgcg tcgtcgccgc cgaagccggt ggtggactgg ctggccaggc
     1921 gggcggcgtt ccagtcggtg gcgctgatcc acaggcggca gccccagcgc tcggtgagcc
     1981 agtgggcgtt gccgatgtgg tcggggtgca tgtgcgtgac gatcacgcgc agcacgggca
     2041 ggccgtccaa ctggtgcgcg aacacctgct cccacgcgcc gcgcgtggcc tcgttggcga
     2101 cgccgcagtc cacgatggcc cagccctcgc gaccgtcgtc cgcccgatcg cgcagcagcc
     2161 acaggttgat gtgatcgagc gcgaacggca gcggcatgcg cagccagcgc acgcccggcg
     2221 cgacatccag caccgcgccc gtggcgggca acgtgtcgcc cagcgggtag gccagtttcg
     2281 attcgtcggc gttggccatg cgggctccgg tggactagac ttccgtttac gttaacgtca
     2341 gtctacccat gagcgcatct cccagtaccg caggcaggaa caaggcgggc aaggcgcccg
     2401 ccgccgccaa gggcgcggcc aagagcttca cgatcacgga gctggccgcc gagttcgacg
     2461 tcaccccgcg cgcgatccgc ttctacgagg acgtgggcct gctggagccg acccgggccg
     2521 gacgcaaccg cgtgtattcg c
//
LOCUS       DATCRO010000852         7070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_121934, whole genome shotgun sequence.
ACCESSION   DATCRO010000852 DATCRO010000000
VERSION     DATCRO010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121934"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..487)
                     /locus_tag="VIN75_23210"
     CDS             complement(62..487)
                     /locus_tag="VIN75_23210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977141.1"
                     /translation="MALLHEKKAHRAARPHAGHAHRHPPIRNILVVDDSVDAADSLGQ
                     LLAAWGYHVTVAHDGPAALAAMQDTQPDIALLDLDMPAMDGYELAAHLRFQPGCEHLP
                     IVAITGSGAPDDVRRSRAKGFSAHLVKPVSAASLRTLLA"
     gene            complement(580..1869)
                     /locus_tag="VIN75_23215"
     CDS             complement(580..1869)
                     /locus_tag="VIN75_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8977142.1"
                     /translation="MTMSPLRWLGLAASMACGLAAAAPNAAADHWVGTWAAAPQAAFP
                     GPLARLDDRTLRLVVHASVGGDRVRVRLSNTYGPQPLRIAAAHVALRGQGADIVDGSD
                     RVLTFGGAPRAVIAPGASITSDPVDLAVPALSELAVSLHAAGRARAGTTHALAQQTSY
                     VATATGDAAGSTHWRAPRRIDDWPFLTGVDVQAPAGGATLVLFGDSWIDGDGSTPDAN
                     ARWGDALAARLQREGGACSRLGVVNSGLVGNRLLHGSPVRHRPHAPDFGRALGESGLA
                     RFDRDVLAQPGARAVLVHLGNNDLGFDGAVAPAGETVSLRSLVAAYRELVARAHAGGL
                     RAIGTTLTPVEGTTLLPGYDSPAKEALRQQVNAWIRDGGEFDAVIDVDRIVRDPDRPS
                     RLLARLASPDHLHPNDAGYAAVAAAFPVALCGDLAAR"
     gene            2070..2468
                     /locus_tag="VIN75_23220"
     CDS             2070..2468
                     /locus_tag="VIN75_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977143.1"
                     /translation="MFRHSRPHPHVHIVRWHGSRAVRRVTFGGLLILLGIGSLLRSQG
                     VITPDELWLALPGAIALSGVVRLVTCPSAASVVGALIRFAIAAYAVVVIEHIGGWTLA
                     ATWPVLLIAVGTGMVAQALLGQRWHEEPNW"
     gene            2465..3208
                     /locus_tag="VIN75_23225"
     CDS             2465..3208
                     /locus_tag="VIN75_23225"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY8977144.1"
                     /translation="MSSDPMNNDPFNRDPMRRQRSRVVLGIFIIAVGVFALLDNLHLF
                     DSHLVQPFWPLVFVALGALRLSQPQHARQWLFGFVLIGVGAAMTLQNLGVIHFQWRDL
                     WPLALIWVGLAVVTKGIVFSRGSPDCPPGRRVELTEHGARIDASATMSGIVLKNDSQE
                     FAGGEVSAVMGAVEIDLRQAAIATEATLHLSVIMGGVEIRVPREWSVVVNGSPVLGGI
                     EDKTVPPMVPGKRLVIGGSIIMGGVEITN"
     gene            3208..4275
                     /locus_tag="VIN75_23230"
     CDS             3208..4275
                     /locus_tag="VIN75_23230"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8977145.1"
                     /translation="MHPLLADPRKLLFYELAWLAAGIGFAALLRLDEQADWIGALCFT
                     LPLALVWSFDVLSAYYVCRSQPYRQRRWPVTLSLFGGASLLSGLVFLGAGETLNYIGG
                     DIVRGHAFVTMPSAAWVMYFLGGFALYLLSLLAHDVLIAFETVQDAARREAESRVLAR
                     DAELQMLRTQINPHFLFNSLNSISALTGFDPAAAREMTVDLAQFFRRTLALAERERIT
                     LGEEVTLSEHFLAIEKRRFGKKLGAELRVSPEAAQCLLPPMTLQPLLENAIKHGIRQV
                     DGGGTIEVEALVRDGWLHIAVVNPVAREDTPSSGHGVGLKNIRERLAVLYGQRARIAW
                     QRGESSFTIEINLPVDKETGT"
     gene            4272..5039
                     /locus_tag="VIN75_23235"
     CDS             4272..5039
                     /locus_tag="VIN75_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977146.1"
                     /translation="MTSTSRLRALVVDDEDLARHLILEYLKGHADVEVVGECENGLDA
                     VKQIGALNPDLVFLDIQMPRLTGLEVLELTGRRSGVIFTTAYDEHAIKAFELHAVDYL
                     LKPFSKARFDDALARARTLHASANANANAPQAPAQGVDALVARRGAALERILIRDRDQ
                     VHVIPVEQVECIEAQGDYLAIEVDGKCHLKPQRISEIEEQLDPTRFLRVHRSFIINLA
                     HLQAIERPGPDRHAARLRSGKRVPISRSGYEKLRELV"
     gene            5114..6448
                     /locus_tag="VIN75_23240"
     CDS             5114..6448
                     /locus_tag="VIN75_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY8977147.1"
                     /translation="MSRLPAALALATAVGAALSAHAVPGETRVGPVPSPADTVGPHPV
                     IGPVDKHLIPTMQIAPDKGWPVNAKPLAPAGVAVNAFATNLNHPRWLYVLPDGDVLVA
                     ETNAPPKPEDAKGLKGKVMKAVQKKAGAGAPTANRIVLLRDADGDGVAETKTVFIDAL
                     NSPFGMVLVGNDLYVADSDAVLKFAYKPGETSIAEPGVKVTDLPAGPINHHWTKNIIA
                     SPDGSKLYATTGSNSNAGDNGIAAERGRACIWEIDRATGQKRPYATGIRNPNGMAWVG
                     DVLWTVSNERDELGNDLPPDFLTSVKPGGFYGFPWSYWGQHVDDRVKPANPAMVAKAI
                     APDYALGAHVAALGLVAAPKTALPAPFAGGMFVGEHGSWNRKPPVGYKVVFVPFADGH
                     PSGMPVDVLTGFVNDQGDAYGRPVGVAIDDKGALLVADDVGNTVWRATGAAR"
BASE COUNT         1022 a         2540 c         2500 g         1008 t
ORIGIN      
        1 cgcttggagg ccgccgcttc ggggtgaacc ctttcggtgc tatcccgatc cgcgggcctg
       61 cctacgcgag aagcgtgcgc aggctcgcgg ccgacaccgg cttgaccagg tgcgccgaga
      121 accccttggc gcgcgagcgc cggacgtcgt cgggcgcgcc cgagccggtg atcgccacga
      181 tgggcaggtg ctcgcagccc ggctggaagc gcaggtgtgc ggccagctcg tagccgtcca
      241 tcgcgggcat gtcgaggtcc aggagcgcga tgtcgggctg cgtgtcctgc atcgcggcga
      301 gcgccgcggg accgtcgtgg gccacggtga cgtggtagcc ccaggccgcg agcagctggc
      361 cgagggaatc ggctgcatcg acgctgtcgt cgaccaccag gatgttgcgg atcggggggt
      421 gtcgatgcgc gtggccggcg tggggtcgcg cggcgcggtg cgctttcttc tcgtgaagga
      481 gggccatgtc gttcttcgaa tcggtcgcca ggctcggagg gcctctgaat gcggttccgg
      541 cgggacatga gcaatccgga tgcctgcgcg gacgaacgtt cagcgcgccg ccagatcgcc
      601 gcatagggcg accgggaatg ccgccgcgac ggccgcatag cccgcgtcgt tcggatgcag
      661 gtgatcgggg ctggcaaggc gcgcgagcag gcgcgacggg cgatccggat cgcgcacgat
      721 gcggtcgacg tcgatcaccg cgtcgaactc gccgccgtcg cggatccacg cgttgacctg
      781 ctggcgcagc gcctccttgg cgggcgagtc gtagccaggc agcagcgtcg tgccctccac
      841 cggcgtcagc gtcgtcccga tcgcgcgcag gccgccggcg tgcgcgcgcg ccacgagctc
      901 gcgataggcc gcgacgagcg accgcaggga caccgtctcg ccggccggcg cgaccgcgcc
      961 gtcgaagccg aggtcgttgt tgcccaggtg cacgagcacc gcgcgcgcgc cgggctgcgc
     1021 cagcacgtcg cggtcgaagc gcgcgaggcc cgattcgccg agcgcgcgcc cgaagtccgg
     1081 cgcatggggc cgatggcgca ccggactgcc atgcagcagc cggttgccca ccaggccgga
     1141 gttgacgacg cccaggcgcg agcaggcacc gccctcgcgc tgcaggcgcg cggcgagcgc
     1201 gtcgccccag cgcgcgttcg cgtcgggcgt gctgccgtcg ccgtcgatcc acgaatcgcc
     1261 gaacaggacg agcgtcgcgc cgcccgcggg cgcctggacg tccacgccgg tgaggaaggg
     1321 ccagtcgtcg atgcggcgcg gcgcgcgcca gtgcgtgctg ccggcggcgt cgccggtcgc
     1381 ggtcgcgacg tagctcgtct gctgcgcaag agcgtgggtc gtgccggcgc gcgcgcggcc
     1441 ggccgcatgc aggctcaccg cgagctcgga caatgccggc accgccagat ccacgggatc
     1501 gctcgtgatg ctggcgcccg gcgcgatgac ggcccgcggc gcgccgccga acgtcagcac
     1561 gcggtcgctg ccgtcgacga tgtcggcgcc ctgcccgcgc aaggcgacgt gcgccgccgc
     1621 gatgcgcagc ggctgcggcc cgtaggtgtt ggacaggcgc acgcggacgc gatccccgcc
     1681 gacgctggca tgcaccacca gccgcagcgt gcggtcgtcc aggcgtgcga gcggccccgg
     1741 gaacgcggcc tgcggcgcgg cagcccaggt gccgacccag tgatcggcgg ccgcgttcgg
     1801 ggcggccgcg gccaggccgc acgccatgga ggcggccagc ccgagccagc gcagcggtga
     1861 catcgtcatg cggcgacgct atcaaaggcc cgggcggccg ctcgcgcgcc tgcgacgaag
     1921 cgcggatcca cgggggcgaa tggccgggca gggctcaccc cgacctcgat tcgtcgccat
     1981 ttcggggcca ttggtcgatt cgcgcgtgcc gggccgcggg gcttctgcga tgctgtccat
     2041 ccatcgagaa ccgccttcag gattgccgca tgttccgcca ctcgcgtccc catccccatg
     2101 tacacatcgt caggtggcac ggctcgcgcg ccgtccgccg cgtcacgttc ggcggcctgc
     2161 tgatcctgct gggcatcggc agcctgctgc gcagccaggg cgtgatcacg cccgacgagc
     2221 tgtggctggc cttgcccggc gcgatcgcgc tgtccggcgt ggtgcgcctg gtcacctgcc
     2281 cgagcgccgc cagcgtcgtg ggcgcgctca tccgcttcgc gatcgcggcg tacgcggtgg
     2341 tcgtcatcga gcacatcggc ggctggacgc tcgcggccac ctggccggtg ctgctgatcg
     2401 ccgtgggcac gggcatggtc gcgcaggcgc tgctgggcca gcgctggcac gaggagccga
     2461 actggtgagc tccgatccca tgaacaacga cccgttcaac cgcgacccga tgcggcgcca
     2521 gcgcagccgc gtcgtcctcg gcatcttcat catcgcggtg ggcgtcttcg cgctgctgga
     2581 caacctgcac ctgttcgaca gccacctcgt ccagccgttc tggccgctgg tgttcgtggc
     2641 gctgggcgcg ctgcggctgt cgcagccgca gcatgcgcgc cagtggctgt tcggcttcgt
     2701 gctgatcggg gtcggcgcgg cgatgacgct gcagaacctc ggcgtgatcc atttccagtg
     2761 gcgcgacctg tggccgctgg cgctgatctg ggtgggcctg gccgtggtca ccaagggcat
     2821 cgtgttcagc cgcggcagcc ccgactgccc gcccggccgg cgcgtcgagc tgaccgagca
     2881 cggcgcccgc atcgacgcct cggccacgat gagcggcatc gtgctgaaga acgactcgca
     2941 ggagttcgcc ggcggcgagg tcagcgcggt catgggcgcc gtcgagatcg acctgcgcca
     3001 ggccgccatc gccaccgagg cgacgctgca cctgtcggtc atcatgggcg gcgtggagat
     3061 ccgcgtgccg cgcgaatggt cggtggtggt caacggctcg ccggtgctgg gcgggatcga
     3121 ggacaagacc gtcccgccga tggtgcccgg caagcgcctg gtcatcgggg gttcgatcat
     3181 catgggcggc gtggaaatca ccaactgatg cacccgctgc tggccgatcc tcgcaagctg
     3241 ctcttctacg agctcgcctg gctggccgcc ggcatcggct ttgccgccct gcttcggctg
     3301 gacgagcagg ccgactggat cggcgcgctg tgcttcacgc tgccgctcgc actggtgtgg
     3361 tccttcgacg tgctctcggc ctactacgtc tgccgcagcc agccttaccg gcagcgccgc
     3421 tggccggtga cgctgagcct gttcggcggc gcgtcgctgc tgtcggggct cgtgttcctg
     3481 ggcgcgggcg agacgctcaa ctacatcggc ggcgacatcg tgcgcgggca cgccttcgtc
     3541 acgatgccgt cggcggcctg ggtgatgtac ttcctcggcg gcttcgcgct gtacctgctg
     3601 tcgctgctgg cgcacgacgt gctgatcgcc ttcgagaccg tgcaggacgc cgcgcgccgc
     3661 gaggccgagt cgcgcgtgct cgcgcgcgac gccgagctgc agatgctgcg cacgcagatc
     3721 aacccgcact tcctgttcaa cagcctcaac tcgatcagcg cgctcaccgg gttcgacccc
     3781 gccgccgcgc gcgagatgac ggtcgacctc gcgcagttct tccgccgcac gctggcgctg
     3841 gccgagcgcg aacgcatcac gctgggcgag gaggtgacgc tcagcgagca cttcctcgcg
     3901 atcgagaagc gccgcttcgg caagaagctc ggcgccgagc tgcgcgtcag ccccgaggca
     3961 gcgcaatgcc tgctgccgcc gatgacgctg cagcccctgc tggaaaatgc gatcaagcac
     4021 ggcatccggc aggtggacgg cggtggcacc atcgaggtcg aagcgctggt gcgcgacggc
     4081 tggctgcaca tcgcggtcgt caatccggtg gcgcgcgagg acacgccgtc cagcggccac
     4141 ggcgtcggcc tgaagaacat ccgcgaacgc ctggccgtgc tgtacggcca gcgcgcccgc
     4201 atcgcgtggc aacgcggcga atcgagcttc accatcgaga tcaacctgcc cgtcgacaag
     4261 gagaccggca catgacatcc acttcccgcc tgcgcgcgct cgtcgtcgac gacgaggatc
     4321 tcgcgcgcca cctgatcctc gaatacctga aggggcatgc ggacgtcgag gtcgtcggcg
     4381 aatgcgagaa cggcctggac gccgtcaagc agatcggcgc gctcaacccc gacctcgtgt
     4441 tcctggacat ccagatgccg cgcctgaccg gcctggaggt gctggagctc accggccgcc
     4501 gctcgggcgt catcttcacc accgcctacg acgagcacgc gatcaaggcg ttcgagctgc
     4561 acgcggtgga ctacctgctc aagccgttct cgaaggcgcg cttcgacgac gccctcgcgc
     4621 gcgcccgcac gctgcacgcg agcgcgaacg ccaacgcgaa cgcgccgcag gcgcccgcgc
     4681 aaggcgtcga cgcgctcgtg gcgcgccgcg gcgcggcgct ggaacgcatc ctgatccgcg
     4741 accgcgacca ggtgcatgtc atcccggtcg aacaggtcga gtgcatcgag gcgcagggcg
     4801 actacctcgc catcgaggtc gacggcaagt gtcatctcaa gccgcagcgc atctcggaga
     4861 tcgaggagca gctcgacccc acgcgcttcc tgcgcgtgca ccgctcgttc atcatcaacc
     4921 tcgcgcacct gcaggccatc gagcgccccg gccccgaccg ccatgccgcg cgcctgcgca
     4981 gcggcaagcg cgtgccgatc agccgcagcg gctacgagaa gctgcgcgag ctggtctgag
     5041 ggcgcaacgg gcacgggaac atcgattgca ggaccatggc tccgctgcgc gagcagcctg
     5101 tccaggagcc acgatgtccc gcctgcccgc cgccctcgcg ctcgccaccg ccgtcggggc
     5161 cgcgctgtcc gcccacgcgg tgcccggcga gacgcgcgtc gggccggtgc cctcgcccgc
     5221 cgacacggtg ggcccgcacc cggtcatcgg gccggtggac aagcacctga tcccgacgat
     5281 gcagatcgcg cccgacaagg gctggccggt gaacgccaag cccctcgcgc ccgccggcgt
     5341 cgccgtcaac gcattcgcca cgaacctgaa ccatccgcgc tggctctacg tgctgcccga
     5401 cggcgacgtg ctggtggccg agaccaacgc gccgcccaag cccgaggacg ccaagggcct
     5461 gaagggcaag gtcatgaagg cggtgcagaa gaaggccggc gccggcgcgc ccaccgccaa
     5521 ccgcatcgtg ctgctgcgcg acgccgacgg cgacggcgtg gccgagacga agaccgtctt
     5581 catcgacgcc ctgaactcgc cgttcggcat ggtgctggtg ggcaacgacc tgtacgtggc
     5641 cgacagcgac gccgtgctca agttcgccta caagcccggc gagaccagca tcgccgagcc
     5701 cggcgtgaag gtgaccgacc tgcccgccgg gccgatcaac caccactgga ccaagaacat
     5761 catcgcgtcg cccgacggca gcaagctgta cgcgacgacc ggctccaaca gcaacgccgg
     5821 cgacaacggg atcgccgccg aacggggccg cgcgtgcatc tgggagatcg accgcgccac
     5881 cggccagaaa cgcccgtacg ccaccggcat ccgcaacccg aacggcatgg cctgggtcgg
     5941 cgacgtgctg tggacggtgt ccaacgagcg cgacgagctg ggcaacgacc tgccgcccga
     6001 cttcctgacc tcggtcaagc cgggcgggtt ctacggcttc ccttggagct actggggcca
     6061 gcacgtcgac gatcgcgtca agccggccaa cccggcgatg gtcgccaagg cgatcgcgcc
     6121 cgactacgcg ctgggcgccc acgtcgccgc actcggcctg gtcgccgcgc cgaagacggc
     6181 cctgcccgcg cccttcgccg gcggcatgtt cgtcggcgag cacggctcgt ggaaccgcaa
     6241 gccgccggtg ggctacaagg tcgtcttcgt gcctttcgcc gacggccatc cctcgggcat
     6301 gccggtggac gtgctgaccg gcttcgtcaa cgaccagggc gacgcctacg gccgcccggt
     6361 gggcgtggcg atcgacgaca agggcgcgct gctggtggcc gacgacgtcg gcaacaccgt
     6421 gtggcgggcg acgggggcgg cgcgttgagg ttggcgcagg gccagcatgc cgagttgtct
     6481 gcgccttccg gcggacttcg cgggccagct tgccgaggcg cctggtcttg aatgccgcac
     6541 gccgaggcag cagggacgct gcttcgccga tcgtgcggca tgcgagacct gacaccgaac
     6601 gtgcgacgtg gcctccgacg ttcggtgtca ggtctcgcat gtcgcacggc tgacggtccg
     6661 gcctttcgcg ggcggtccgg cgtgcgacat gcaacaccag acacctcggg cgcgtgctga
     6721 ccggcagaca gatcaaggcg tgcccactcg ccgaggtgtc tggtcttgaa tgccgcacgc
     6781 ccagacatcg gcgatcctgc ttcgccgatc gtgcggcatg cgagacctga caccgaacgt
     6841 gcgacgtggc ctccgacgtt cggtgtcagg tctcgcatgt cgcacggctg acggtctggc
     6901 ctttcgcggg cagcccggcg tgcgacatgc aacaccagac acctcgggcc cgtgctggcc
     6961 ggcagacaga tcaaggcgtg cccactcgcc gaggtgtctg gtcttgcatg acgcacgcca
     7021 aaacgtcggc gatctgcttc gccgatcgtg cggcatgcga gacctgacac
//
LOCUS       DATCRO010000853         9074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_391419, whole genome shotgun sequence.
ACCESSION   DATCRO010000853 DATCRO010000000
VERSION     DATCRO010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391419"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VIN75_23245"
     CDS             complement(<1..896)
                     /locus_tag="VIN75_23245"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977148.1"
                     /translation="MFNRRPPPPPAVMPTRQSGPDFASAIRDIAGKASELGRGAAELD
                     GTIEDVVASGSRQVTEIGQLAADMQAMTASNRAIESSGEASRVAMGQARDAVAQVGQG
                     VLGVVTSLRDVASAATDITQIALQTRLVAFNATVEAKRAGEAGAGFAVVAEAVKDLAA
                     KVEQSSKLIMSTVTQLDARIDSLAASITDTKDAATHSAFHQALAQVEASVNEIVAATQ
                     RNLQTCQGVATEVGTVAASVAANAETLKRVNANTRSFLQASEALAEITNDCGAVTTDT
                     PFIEAVQRGAARLSALLEQAVAG"
     gene            complement(1013..2203)
                     /locus_tag="VIN75_23250"
     CDS             complement(1013..2203)
                     /locus_tag="VIN75_23250"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY8977149.1"
                     /translation="MIIALSRQRIASIDAFRGLTILVMVFVNQLAGVSGVPQWARHMK
                     ADADAMSFVDVVFPAFLFIVGMSIPFALNQRQARGDDGAALQRHVLARSLGLIVLGFF
                     MVNAEDGFNPARMPFNIGWWSLGLYLCAFLVWGVYGRGEPGGVNTWRAAGMLGLLVLC
                     CTYRGGPDGKAWMTHQWWGILGLIGWSYLITASVYLASRARIAVLLFAAAACIAVYAL
                     CHAFPAATYPDWSDLTDCGGLVCETSLTLLGTVTSLVFFARPEEVAPLRRFGQAGLLV
                     VACVAAGALLRPAYTISKIYASPTWCLYSAAICIALFAALYVVIDQRGHARWTRWLAP
                     VASQPLVAYLIPFVVVGLEDAFGLPLPDALNHGPVGVGFCVVFALFVLGATALVTRRI
                     RLQL"
     gene            complement(2255..5116)
                     /locus_tag="VIN75_23255"
     CDS             complement(2255..5116)
                     /locus_tag="VIN75_23255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8977150.1"
                     /translation="MSFRLNPFASAALLMTGAIPHAFAQQAAPAPQAASAAATAAAAP
                     VEETQTVEVKGIRASYASSLGNKKSADAVVEVVTAEDVGKLPAKNVADTVQRLPGVNI
                     SSNSGGDGAFADANRVSIRGTAPSLTQTLVNGHSVGSADWFIANQGVNGGASGRSVSY
                     DLLPAEIVGKVTVYKTSQADLIEGGATGSVDIETRSPLSLTKQVTVDGSINAVYSTST
                     RKTDPQVSGLVGWKNDAKTFGVLLQAFSERRHDRRDGQEFLGYNTVSFQDIFGLAPID
                     NPNNDPAIAAQNAANAAALAAKQAQYPGLDGATFANNVNATLLEMNMQRKGAMVDLEL
                     KPTNRLRLDFNGFYSKLTETNQDSSLETEQTALIGMGVLPDSYQVNSGGLLTQATWNQ
                     ASMPDYASTGAYVADYDKYYRPGENARVYYADIDLDFAASDTLRITGKLGKTQAVGAT
                     PNEYAYVTNVGNTGLSYKMNGLLMPADVSFPGYADTGNYNDPHLFLAYDGLNKVKSTD
                     QETYFQTDAEWATETGVLDALKFGLRLTSHQRSVLDYYNDGCEAVIPDDTYTQCNGIA
                     QWAGQVENRYGQGLHGGPGFLTNIWQLSPSEITSYVNAPGNLPASSLPYSWPSSFVVN
                     ERTQALYGMAKLVGTQWKGNAGLRVVRTQQVAHYNEGSDAAAPGDTIYPDPHNGDYIH
                     VTTSKTYTDLLPSANLSYDFTNKLIGRFAASRTITRADYTDLAGAVSLNGQNGTGSGG
                     NPQLKPVRSTNWDASLEWYFAPQSLLEVGVFYMDLTSYIGYGQTTILAPNLQQNPIVT
                     TPTYPYTVAQPVNDKGTNKGFELAWQQPIAYGFGINLNYTYALGRDSNGAPLAGSSKH
                     TYNAEGWYENDFLSARLTYSYRSDFLTGIVSALPQYVAGAGDMSASINLKLTDQLSLS
                     FDAKNLTGALARQYVVQKDMPAAIYNNGKQFYLGLKYSM"
     gene            complement(5152..6873)
                     /locus_tag="VIN75_23260"
     CDS             complement(5152..6873)
                     /locus_tag="VIN75_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY8977151.1"
                     /translation="MQVLINHVGFAPSSSKRAIVQHDAPLQAQAFSVVDLRSREVVLG
                     GMTVPVAPVEGWIGRHFAHADLSALTRPGRYAVMLDGNWPPVQSQPFEVGEHLFGSQM
                     LSDIVHYFKGQRCTGLFDDADRRAPLLDTREPRDVHGGWYDASGDCSKYLSHLSYANF
                     MNPQQTPLVTWTLLAGRDRLPPQSKWLDERIVDEALHGADFLVRMQAPEGFFYVTVFD
                     KWSKDERQREICSYATQKGLKSADYQAGFRQGGGMAVAALARAAGAHRDGESTREQYL
                     AAAIRGFELLQARNAEFLDDHAENLIDDYCALLAASELHARTGDARWRDAAAARARAM
                     LARQDDGGFFWCDRARTRSFFHASDAGLPALALMRFLECDPEPALAAHVRAALLRALR
                     FELAITRVEPNPLGYPRQYVAMPGREAATQFFVPHANESGYWWQGENARLGSLATAAR
                     AAQSLALADAAFDADLADYAQGALDWLFGANPFDTCMLQGWGQNPPRYEPGFWNAPGG
                     VCNGITSGLADENDIDFRKPEETEPAHSWRWTEQWIPHAAWLFCALAHGRPRPERSFH
                     PIDAGRR"
     gene            complement(6861..7835)
                     /locus_tag="VIN75_23265"
     CDS             complement(6861..7835)
                     /locus_tag="VIN75_23265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8977152.1"
                     /translation="MTSQTIPAATPGLAASTAPRVLAFDVGGTWVKYGIVDSGGRLLC
                     ADQLATQSEPDGKALLTRLLAVAEPLVARHAPAGIAFSTLGIIDATEGRLVGAVEAIA
                     GYFGQSPKECFERAFGVPVVVENDGNCVALAEGWTGAAAGVCHYLALTLGTGIGGGIV
                     IDGRLYRGANGAAGEWGYMLVDGRVWEDHASPRGLAAAAEKARPGCGLDAEGVFAARD
                     RGDAEMAAVVTAWFGLLATGLANLLFAFNPSRVVIGGGITARGPAFLQELRAEMRLRL
                     RPEFYRMCDIALASAGNQAGLLGAARLWLTQHGGPATAAASEGTPCRS"
     gene            complement(7847..8752)
                     /locus_tag="VIN75_23270"
     CDS             complement(7847..8752)
                     /locus_tag="VIN75_23270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8977153.1"
                     /translation="MSPQLPALAAPGRRPPGSGRPSARARKEMLLNALVWAIVALTLS
                     PVAFMVYASLSDYNDVVTGHLVDAGFHPHWSNYREMWVNVDFLAFLRNSLVICGITTL
                     LSTFLAALAAYALARFRFRGNHAFSVAVSTTQVIPGMLFFLPLYMLYIQIDDAGLPMM
                     NTYHGMIFLYTAMFTPASIWIMRGFFVSIPRELEEAAIIDGCSRLGAFLRIVLPISAP
                     GLIATASFVFLLAWDEVFFAWVLTSSPDVQTVPVGLRLYMGQHATRYDLMMAAAVVSV
                     IPVLLTFFASQRWFIKGLAAGAVKG"
     gene            complement(8754..>9074)
                     /locus_tag="VIN75_23275"
     CDS             complement(8754..>9074)
                     /locus_tag="VIN75_23275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8977154.1"
                     /translation="RLDGATAWQRFRHITLPALKPVIVIQVMFQIIDNIYSYNIVAMM
                     FGKGAGYPGEWGDLLMPLLTRQSFSYWRFGEGAAVSFVMMAVMLVFVAVWMKTFRRSM
                     TDEA"
BASE COUNT         1361 a         3032 c         3292 g         1389 t
ORIGIN      
        1 cccgccaccg cctgttccag caaggcggac aagcgcgccg cgccgcgctg caccgcttcg
       61 atgaagggcg tgtcggtggt gacggcgccg cagtcgttgg tgatctcggc cagcgcctcc
      121 gacgcctgca ggaagctgcg cgtgttggcg ttcacgcgct tgagcgtctc ggcgttggcc
      181 gcgaccgacg ccgccacggt gcccacctcg gtggcgacgc cctggcaggt ctgcaggttg
      241 cgctgcgtgg ccgcgacgat ctcgttgacg ctggcctcca cctgcgcgag cgcctggtgg
      301 aacgcgctgt gcgtcgcggc gtccttggtg tcggtgatgc tcgcggccag gctgtcgatg
      361 cgcgcgtcga gctgcgtgac ggtggacatg atcagcttgg acgactgttc caccttggcc
      421 gcgaggtcct tgaccgcttc ggccaccacg gcgaagcccg cgcccgcctc gccggcgcgc
      481 ttggcctcga ccgtggcgtt gaacgcgacc agccgcgtct gcagcgcgat ctgggtgatg
      541 tcggtggcgg cgctggccac gtcgcgcagc gaggtgacca cgcccagcac gccctgcccg
      601 acctgcgcca ccgcgtcgcg cgcctggccc atcgccacgc ggctcgcctc gccgctggat
      661 tcgatcgcgc ggttggacgc cgtcatcgct tgcatgtcgg cggccagctg tccgatctcg
      721 gtgacctggc ggctgccgga ggccacgacg tcctcgatcg tgccgtcgag ctcggcggcg
      781 ccgcgcccga gctcggaggc cttgcccgcg atgtcgcgga tcgcgctcgc gaagtccggg
      841 ccggactgcc gcgtcggcat cacggcgggc ggcggcgggg gccgtcgatt gaacatgcag
      901 tctcctggcg cgccgggggt gcccccgggc ggtatttgtt gtcgtcgggg ccagtatgca
      961 aaggatgccg gccggcggca aggcgaaatc cagggacttt gtctcggtct tgtcagagct
     1021 gcagcctgat cctgcgcgtg accagcgcgg ttgctccaag aacgaacagt gcgaacacca
     1081 cgcagaagcc cacgccgacg ggcccgtggt tcagcgcgtc gggcaacggc agcccgaacg
     1141 catcctcgag gcccacgacc acgaacggga tcaggtaggc caccagtggc tgcgacgcga
     1201 cgggcgccag ccagcgcgtc cagcgtgcgt ggccgcgctg gtcgatcacg acgtacagcg
     1261 ccgcgaacag cgcgatgcag atcgcggccg agtacaggca ccaggtcggc gacgcgtaga
     1321 tcttggagat cgtgtacgcc ggccgcagca acgcgccggc ggcaacgcag gcgacgacca
     1381 gcaaccccgc ctggccgaac cggcgcagcg gcgccacttc ctcgggccgc gcgaagaaca
     1441 cgaggctggt gaccgtgccc agcagggtca gcgaggtctc gcagaccagc ccgccgcagt
     1501 cggtcaggtc cgaccagtcg ggatacgtcg ccgccgggaa cgcgtggcac agcgcataga
     1561 ccgcgatgca ggccgcggcc gcgaacagca gcaccgcgat gcgcgcgcga ctcgcgaggt
     1621 agacggaggc cgtgatcagg tacgaccagc cgatcagccc caggatgccc caccactggt
     1681 gcgtcatcca ggccttgccg tccggcccgc cgcggtacgt gcagcacagc accagcagac
     1741 ccagcatgcc ggcggcgcgc caggtgttga cgccgccggg ctcgccgcgc ccgtacacgc
     1801 cccagaccag gaacgcgcac aggtacagac ccaacgacca ccacccgata ttgaacggca
     1861 tgcgcgccgg gttgaagccg tcctccgcgt tgaccatgaa gaacccgagc acgatcaacc
     1921 cgagcgaacg agcgagcacg tggcgctgca acgcggcgcc atcgtcgccg cgcgcctggc
     1981 gctggttcag cgcgaacggg atcgacatcc ccacgatgaa caggaatgcc gggaagacga
     2041 cgtcgacgaa gctcatcgcg tcggcgtcgg ccttcatgtg gcgcgcccac tgcggcacgc
     2101 cgctgacgcc ggcgagctgg ttgacgaaga ccatcacgag gatggtgagg ccgcggaacg
     2161 cgtcgatcga ggcgatgcgc tggcgggaca gggcgatgat cacggggaag gttcctggtt
     2221 cgcccgcgcg cgtgcgtgac gcgcgcggcg gcggtcacat cgagtacttc agtccgaggt
     2281 agaactgctt gccgttgttg tagatggcgg ccggcatgtc cttctgcacc acgtactggc
     2341 gcgcgagcgc gccggtcagg ttcttcgcgt cgaacgacag cgacagctgg tcggtcaact
     2401 tgaggttgat cgacgccgac atgtcgcccg cgcccgccac gtactgcggc agggcgctga
     2461 cgatgccggt gaggaagtcc gaacggtagc tgtaggtcag ccgcgcgctg aggaaatcgt
     2521 tctcgtacca cccctcggcg ttgtacgtgt gcttggacga gccggccagc ggcgcgccgt
     2581 tggaatcgcg gcccagagcg taggtgtagt tcaggttgat gccgaacccg tacgcgatcg
     2641 gctgctgcca ggcgagctcg aagcccttgt tcgtgccctt gtcgttgacc ggctgcgcga
     2701 cggtgtatgg atacgtcggc gtcgtgacga tcgggttctg ctgcaggttg ggcgcgagga
     2761 tcgtggtctg gccgtagccg atgtaggacg tcaggtccat gtagaacacg ccgacctcga
     2821 gcagcgactg cggcgcgaag taccactcga gcgacgcgtc ccagttggtg gagcgcacgg
     2881 gcttcaactg cgggttgccg cccgagccgg tgccgttctg gccgttcagg ctcacggcac
     2941 cggcgaggtc ggtgtagtcg gcgcgcgtga tcgtgcgcga cgcggcgaag cgcccgatca
     3001 gcttgttggt gaagtcgtag ctgaggttgg cgctgggcag caggtccgtg taggtcttgc
     3061 tggtcgtcac gtggatgtag tcgccgttgt gcggatcggg gtagatcgtg tcgccgggcg
     3121 cggccgcgtc ggagccctcg ttgtagtggg cgacctgctg cgtgcgcacg acgcgcagcc
     3181 ccgcgttgcc cttccactgc gtgcccacca gcttggccat gccgtacagc gcctgcgtcc
     3241 gctcgttgac gacgaagctg ctgggccacg agtacggcag cgacgatgcc ggcaggttgc
     3301 ccggcgcgtt gacgtagctc gtgatctcgg aagggctcaa ctgccagatg ttggtgagga
     3361 agcccggccc gccgtgcagc ccctggccgt agcggttctc gacctggccg gcccactgcg
     3421 cgatgccgtt gcactgcgtg taggtgtcgt cggggatcac cgcctcgcag ccgtcgttgt
     3481 agtagtcgag cacgctgcgc tggtggctgg tcaggcgcag gccgaacttg agcgcgtcca
     3541 gcacgccggt ctcggtggcc cactccgcgt ccgtctggaa gtaggtctcc tggtcggtcg
     3601 acttcacctt gttcaggccg tcataggcca ggaacaggtg cgggtcgttg tagttgcccg
     3661 tgtcggcata gcccgggaac gagacgtcgg cgggcatcag caggccattc atcttgtagc
     3721 tcaggccggt gttgccgacg ttggtcacgt acgcgtactc gttgggcgtc gcgccgaccg
     3781 cctgggtctt gccgagcttg ccggtgatgc gcagcgtgtc ggacgcggcg aagtcgaggt
     3841 cgatgtccgc gtagtagacg cgcgcgttct cgccggggcg gtagtacttg tcgtagtcgg
     3901 ccacgtaggc gccggtcgag gcgtagtccg gcatcgaggc ctggttccag gtggcctgcg
     3961 tgagcaggcc gccggaattg acctggtagg agtcgggcag cacgcccatg ccgatcagcg
     4021 cggtctgctc ggtctccagg ctcgagtcct ggttggtctc ggtgagcttg gagtagaagc
     4081 cgttgaagtc cagccgcagc cggttggtgg gcttgagctc gaggtcgacc atcgcgccct
     4141 tgcgctgcat gttcatctcg agcagcgtcg cgttgacgtt gttggcgaag gtcgcgccgt
     4201 cgaggccggg atattgcgcc tgcttggctg cgagcgccgc ggcgttggcg gcgttctgcg
     4261 cggcgatcgc cgggtcgttg ttcgggttgt cgatcggcgc caggccgaag atgtcctgga
     4321 acgacaccgt gttgtagccc aggaactcct gcccgtcgcg gcggtcgtgg cggcgctccg
     4381 agaacgcctg cagcagcacg ccgaaggtct tggcgtcgtt cttccagccg accagcccgc
     4441 tgacctgcgg gtccgtcttg cgcgtggacg tgctgtagac cgcgttgatg gagccgtcga
     4501 cggtgacctg cttcgtcagg ctcagcggcg agcgcgtctc gatgtcgacc gagcccgtgg
     4561 cgccgccttc gatcaggtcg gcctgcgagg tcttgtagac cgtgaccttg ccgacgatct
     4621 cggccggcag caggtcgtag ctgacgctgc gtcccgatgc gccgccgttg accccctggt
     4681 tggcgatgaa ccagtcggcc gagcccacgc tgtggccgtt gaccagcgtc tgcgtgagcg
     4741 acggcgcggt gccgcggatg ctcacgcggt tggcgtcggc gaaggcgccg tcgccgccgg
     4801 agttggagct gatgttgacg ccgggcaggc gctgcacggt gtcggccacg ttcttggccg
     4861 gcagcttgcc cacgtcctcg gcggtgacga cctcgaccac cgcatcggcg ctcttcttgt
     4921 tgccgagcga cgaggcatac gacgcgcgga tgcccttgac ctcgacggtc tgggtctcct
     4981 cgaccggcgc ggcggcagcc gtagccgcgg cggatgcggc ctggggcgcc ggcgcggcct
     5041 gttgcgcgaa ggcgtggggg atcgctccgg tcatcagcag cgccgccgat gcgaaggggt
     5101 tcaagcgaaa ggacatggca gcctcgaaag gcggctacag gcgctccggg gtcagcgtct
     5161 gcccgcgtcg atgggatgga aagaacgttc ggggcgcggt ctcccgtggg ccagcgcgca
     5221 gaacagccac gcggcgtgcg gaatccactg ctcggtccag cgccacgagt gcgccggttc
     5281 ggtctcttca ggcttgcgga aatcgatgtc gttctcgtcg gccaggcccg acgtgatgcc
     5341 gttgcagacg ccgccgggcg cgttccagaa gccgggctcg tagcgcggcg ggttctggcc
     5401 ccagccctgc agcatgcagg tgtcgaacgg gttggcgccg aacagccagt cgagggcgcc
     5461 ctgggcgtag tcggccaggt cggcgtcgaa ggcggcgtcg gccagggcca gcgactgcgc
     5521 ggcgcgtgcc gcggtggcca gcgagccgag gcgggcgttc tcgccctgcc accagtagcc
     5581 gctctcgttg gcgtgcggca cgaagaactg cgtggcagcc tcgcggcccg gcatcgcgac
     5641 gtactggcgc ggatagccca gcgggttggg ctcgacgcgc gtgatcgcca gctcgaagcg
     5701 cagcgcgcgc agcagcgcgg cacgcacgtg ggcggcgagc gcgggttcgg ggtcgcactc
     5761 gaggaatcgc atcagcgcga gcgcgggcag gcccgcgtcg ctggcgtgga agaagctgcg
     5821 cgtgcgggcg cggtcgcacc agaagaagcc gccgtcgtcc tgccgggcca gcatcgcgcg
     5881 ggcgcgcgcc gccgccgcgt cacgccagcg cgcgtccccc gtgcgcgcat ggagctcgct
     5941 ggcggccagc agtgcgcagt agtcatcgat caggttctcc gcgtggtcgt cgaggaactc
     6001 ggcgttgcgc gcctgcagca gttcgaagcc gcggatcgcc gcggccaggt attgctcgcg
     6061 cgtggactcg ccgtcacggt gcgcgcccgc ggcgcgcgcg agcgcggcca cggccatccc
     6121 gccgccctgg cggaacccgg cctggtagtc ggccgacttc aggcccttct gcgtggcgta
     6181 ggagcagatc tcgcgctggc gctcgtcctt gctccacttg tcgaacaccg tgacgtagaa
     6241 gaagccctca ggcgcctgca tgcgcacgag gaagtcggcg ccgtgcagcg cctcgtcgac
     6301 gatgcgctcg tccagccact tggactgcgg cggcagccgg tcgcggccgg ccagcagcgt
     6361 ccacgtcacc agcggcgtct gctgcgggtt catgaagttg gcgtacgaca ggtgcgacag
     6421 gtacttcgag cagtcgcccg acgcgtcgta ccagccgccg tgcacatcgc gcggctcgcg
     6481 ggtgtccagc aacggggcgc ggcggtcggc gtcgtcgaac aggccggtgc agcgctggcc
     6541 cttgaagtag tggacgatgt ccgacagcat ctggctgccg aacaggtgct cgccgacttc
     6601 gaacggctgc gactgcaccg gcggccagtt gccgtccagc atcacggcat agcgcccggg
     6661 acgcgtcaac gcgctcaggt cggcgtgcgc gaagtgcctt ccgatccagc cctcgaccgg
     6721 cgcgacaggc accgtcatgc cgccgagcac gacctcgcgc gaacgcaggt cgaccaccga
     6781 gaacgcctgc gcctgcaacg gcgcgtcgtg ctgcacgatc gcgcgcttgg acgacgacgg
     6841 cgcgaagccg acgtggttga tcaggacctg catggcgtgc cttcgctggc ggccgcggtg
     6901 gccgggccgc catgttgcgt gagccacagc cgcgccgcgc ccagcagccc ggcctggttg
     6961 ccggccgagg cgagcgcgat gtcgcacatg cggtagaact cggggcgcag gcgcagccgc
     7021 atctcggcgc gcagctcctg caggaaggcc gggccgcgcg cggtgatgcc gccgccgatg
     7081 accacgcgcg aggggttgaa cgcgaagagc aggttcgcga ggcccgtggc gagcaggccg
     7141 aaccacgcgg tgacgacggc ggccatctcg gcatcgccgc ggtcgcgcgc ggcgaacacg
     7201 ccctcggcat cgaggccgca acccgggcgc gccttctcgg cggccgctgc gaggccgcgc
     7261 ggcgaggcgt ggtcttccca cacacggccg tccaccagca tgtagcccca ctcgccggcc
     7321 gccccgttcg cgccgcggta caggcggccg tcgatgacga tgccgccgcc gatgccggtg
     7381 cccagcgtca gcgcgaggta gtggcacacg ccggcggccg cgcccgtcca gccttcggcc
     7441 agcgcgacgc agttgccgtc gttctccacg accaccggca cgccgaatgc gcgctcgaag
     7501 cactccttgg gcgactgccc gaagtagccg gcgatggcct cgacggcgcc gacgagccgg
     7561 ccttcggtcg cgtcgatgat gcccagcgtg gagaacgcga tgccggccgg tgcgtgtcgc
     7621 gccaccagcg gctcggcgac cgccagcagc cgcgtgagca acgccttgcc gtcgggctcg
     7681 ctctgcgtgg ccagctggtc ggcgcacagc aggcggccgc ctgagtcgac gatgccgtac
     7741 ttgacccagg tgccgccgac gtcgaacgcg agcacgcgcg gggcggtgga cgcggcgagg
     7801 ccgggggtgg cggcggggat ggtctgagaa gtcatgggaa tgggaatcag cctttgacgg
     7861 caccggccgc caggcccttg atgaaccagc gctgcgaggc gaagaaggtg agcaggacgg
     7921 ggatcacgct gacgacggcc gcggccatca tcaggtcgta gcgggtggcg tgctggccca
     7981 tgtacaggcg cagtcccacg ggcacggtct gcacgtcggg cgacgacgtc agcacccagg
     8041 cgaagaacac ctcgtcccag gccagcagga acacgaacga ggcggtggcg atcaggcccg
     8101 gcgcgctgat cggcagcacg atgcgcagga aggcgcccag gcgcgagcag ccgtcgatga
     8161 tcgcggcctc ctcgagctcg cgcgggatcg acacgaagaa gccgcgcatg atccagatgc
     8221 tggccggcgt gaacatcgcc gtgtacagga agatcatgcc gtggtacgtg ttcatcatcg
     8281 gcagcccggc atcgtcgatc tggatgtaga gcatgtacag cggcaggaag aacagcatgc
     8341 cggggatgac ctgcgtcgtg ctcaccgcga cgctgaacgc gtggttgccg cggaaccgga
     8401 agcgggccag cgcgtaggcc gccagcgcgg ccaggaaggt ggacagcagc gtcgtgatgc
     8461 cgcagatcac caggctgttg cgcaggaacg cgaggaagtc gacgttgacc cacatctcgc
     8521 ggtagttcga ccagtgcggg tggaacccgg catccaccag gtggccggtg acgacgtcgt
     8581 tgtagtccga cagcgacgca tagaccatga acgccacggg cgacagcgtg agggcgacga
     8641 tcgcccacac cagcgcgttg agcagcattt ccttcctcgc ccgggcgctg ggccgccccg
     8701 agccgggcgg gcgccgcccg ggggccgcga gcgcggggag ctgggggctc acgtcacgcc
     8761 tcatcggtca tgctccggcg aaacgtcttc atccacaccg cgacgaacac gagcatcacg
     8821 gccatcatca cgaacgacac cgcggcgccc tcgccgaagc gccagtagct gaagctctgg
     8881 cgcgtgagca gcggcatcag caggtcgccc cactcgccgg ggtagccggc gcccttgccg
     8941 aacatcatcg ccacgatgtt gtacgagtag atgttgtcga tgatctggaa catcacctgg
     9001 atgacgatca cgggcttgag cgcgggcagc gtgatgtggc ggaagcgctg ccacgccgtg
     9061 gcgccgtcca ggcg
//
LOCUS       DATCRO010000854         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_729688, whole genome shotgun sequence.
ACCESSION   DATCRO010000854 DATCRO010000000
VERSION     DATCRO010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729688"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VIN75_23280"
     CDS             <1..562
                     /locus_tag="VIN75_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8977155.1"
                     /translation="FVTAYDQYALQAFERAAVDYLQKPVQPARLAACCARLRDTLALR
                     AAASGSASGATAPDDAALLGRLRSLLGAPAEAAPAALTILQASIGATLHMIPVDEVVY
                     FEAADKYVRVVTADREALIRMSLRELLPRLRPDKFWQIHRGTVVRADAIATATRDDSG
                     KVFLTLRQRPERLVASRLYAHLFRGM"
     gene            complement(492..932)
                     /locus_tag="VIN75_23285"
     CDS             complement(492..932)
                     /locus_tag="VIN75_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977156.1"
                     /translation="MQQAMTMTMSATARWIDTGAQVLIGATAIVAACIALTGAGMGRH
                     GDAPQGRFRMAMEAYEAQQWPQAYAQLSAAADAGDPAAARVATMMARQGPLLFGQHFD
                     VSAERLQRWDQAMRGKTPPAAVTSRGTDARTDGSQPGVQVAGAR"
     gene            1073..2878
                     /locus_tag="VIN75_23290"
     CDS             1073..2878
                     /locus_tag="VIN75_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY8977157.1"
                     /translation="MNTPKNPTPAAPADEAVKPANFLRNVIERDLEAGTYAHRQWGGG
                     AGDAAFHAAGTPDAARVRLRFPPEPNGYLHVGHAKSICLNFGLARDYGGVCHLRFDDT
                     NPEKEDQEYVDGIKENVRWLGFDWVTPSGDYLFYASDYFDFMYRAAEALVQAGLAYVD
                     EQSADQIRANRGDFNTPGTDSPFRGRTPAENLARLREMRDGKHADGSMILRARIDMAS
                     PNINLRDPALYRIRRAHHHNTGDRWCIYPMYTFAHPIEDALERITHSICTLEFEDQRP
                     FYDWLLERLVDLGLLAHPLPRQYEFARLNMTYIVTSKRKLRQLVEENHVTGWDDPRMP
                     TLVGMRRRGYTAGALQLLAERTGATKNNTWIDYAWLDIALRDDLEDKAARAMAVLDPI
                     PLKLTNWAEVFGSDAHREPCGAPAHPHHPEMGQREFVLGPQVWIERDDFLETPVKGYQ
                     RLFPGNKVRLKYGVIVECTGCEKDADGKLTAVLATVVPDTKSGTPGADAVKVKGTITW
                     VAAHDGVPVTLNLYDRLFAVPQPGAADAPLTAELNPASWRATQGYVEPSAGQLAAETR
                     VQFERHGYFVTDRVDHQPGKPVFNRITTLKDSWGK"
     gene            2976..3296
                     /locus_tag="VIN75_23295"
     CDS             2976..3296
                     /locus_tag="VIN75_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977158.1"
                     /translation="MTAALKNRIENLIAMAQLLERVDANPTIVGAQQYLNLVNTVKGL
                     LGEDDLPDDALRAVLRACPASAMLYENMHYDRSGLSQSPLDAAVASEMAAADLIAGLR
                     ARAH"
BASE COUNT          540 a         1264 c         1117 g          447 t
ORIGIN      
        1 gttcgtcacc gcctatgacc agtacgcgct gcaggccttc gagcgggcgg ccgtcgacta
       61 cctgcaaaag cccgtccagc cggcgcggct ggcggcgtgc tgcgcgcgcc tgcgcgacac
      121 gctggcgctg cgcgccgcgg ccagcggttc ggcgagcggc gcaaccgcgc ccgacgacgc
      181 ggccctgctg ggccggctgc gctcgctgct gggcgcgccg gccgaggcgg cgcccgcggc
      241 gctgaccatc ctgcaagcga gcatcggcgc cacgctgcac atgattccgg tcgacgaggt
      301 cgtctacttc gaggcggcgg acaagtacgt gcgcgtggtc accgccgatc gcgaggcgct
      361 gatccggatg tcgctgcgcg agctgctgcc gcggcttcgg cccgacaagt tctggcagat
      421 ccaccgcggc accgtggtgc gcgccgacgc gatcgccacg gccacgcgcg acgactcggg
      481 caaggtgttc ctcaccttgc gccagcgacc tgaacgcctg gttgcgagcc gtctgtacgc
      541 gcatctgttc cgcgggatgt gacggcggcg ggaggcgtct tgccgcgcat cgcctggtcc
      601 cagcgctgca ggcgctcggc gctgacgtcg aagtgctgcc cgaacagcag cgggccctgg
      661 cgcgccatca tcgtggccac gcgcgcggcg gcggggtcgc ccgcatcggc cgcggccgac
      721 agctgcgcat aggcctgcgg ccactgctgc gcctcgtagg cttccatcgc catgcgaaag
      781 cgcccctgcg gcgcgtcgcc gtgacgtccc atgccggcgc cggtcagcgc gatgcaggcc
      841 gcgacgatcg ccgttgcgcc gatcagcacc tgggcgccgg tgtcgatcca gcgggccgtc
      901 gcactcatcg tcatcgtcat cgcttgctgc atggtcttct cctgccgtgg cgggccgaaa
      961 cttccggctt gtcgaggcgc gagtatggaa aacggccgcc cgctcagcta tcccccttga
     1021 gaggggacgg tgcggcgcca cctgtaacgg gcgaccgtag aattgccgcg acatgaacac
     1081 gcccaagaac cccacccccg ccgcgccggc cgacgaggcc gtcaagcccg cgaacttcct
     1141 gcgcaacgtc atcgaacgcg atctcgaggc gggcacgtac gcgcaccggc agtggggcgg
     1201 cggtgccggg gatgcggcct tccacgcggc gggcacgccg gacgcggcgc gcgtgcgcct
     1261 gcgcttcccg cccgagccca acggctacct gcacgtgggc cacgccaaga gcatctgcct
     1321 gaacttcggg ctggcgcgcg actacggcgg cgtctgccac ctgcgcttcg acgacaccaa
     1381 cccggagaag gaagaccagg agtacgtcga cggcatcaag gagaacgtgc gctggctggg
     1441 cttcgactgg gtcacgccgt ccggcgacta cctgttctac gccagcgact acttcgactt
     1501 catgtatcgc gccgccgagg cgctggtgca ggccggcctc gcctacgtcg acgagcagtc
     1561 cgccgaccag atccgcgcca accgcggcga cttcaacacg ccgggcaccg acagcccgtt
     1621 ccgcggccgc acgccggccg agaacctggc ccgcctgcgc gagatgcgcg acggcaagca
     1681 cgcggacggc tcgatgatcc tgcgggccag gatcgacatg gccagcccca acatcaacct
     1741 gcgcgacccg gcgctgtacc gcatccgccg cgcgcaccac cacaacaccg gcgaccggtg
     1801 gtgcatctac ccgatgtaca cgttcgcgca cccgatcgag gacgcgctcg agcgcatcac
     1861 gcacagcatc tgcacgctcg agttcgagga ccagcgcccg ttctacgact ggctgctgga
     1921 acgcctggtc gacctcggcc tgctggcgca tccgctgccc aggcagtacg agttcgcgcg
     1981 cctgaacatg acctacatcg tcaccagcaa gcgcaagctg cgccagctgg tcgaggagaa
     2041 ccacgtcacc ggctgggacg acccgcgcat gcccacgctg gtcggcatgc gccgccgcgg
     2101 ctatacggcc ggcgcgctgc aactgctggc cgagcgcacc ggtgccacca agaacaacac
     2161 gtggatcgac tacgcctggc tcgacatcgc gctgcgcgac gacctcgagg acaaggccgc
     2221 ccgtgcgatg gccgtgctcg acccgatccc gctcaagctc accaactggg ccgaggtctt
     2281 cggcagcgac gcccaccgcg agccctgcgg cgcgccggcg cacccgcacc accccgagat
     2341 gggccagcgc gagttcgtcc tcggcccgca ggtctggatc gagcgcgacg acttcctcga
     2401 gacgccggtc aagggctacc agcgcctgtt ccccggcaac aaggtgcgcc tgaagtacgg
     2461 cgtcatcgtc gagtgcaccg gctgcgagaa ggatgccgac ggcaagctca ccgccgtgct
     2521 cgccaccgtc gtgcccgaca ccaagagcgg cacgccgggc gccgacgccg tcaaggtcaa
     2581 gggcaccatc acctgggtgg ccgcgcacga cggcgtgccc gtcacgctca acctgtacga
     2641 ccgcctgttc gcggtgccgc aacccggcgc agcggacgcc ccgctcacgg ccgagctgaa
     2701 cccggccagc tggcgcgcca cccaaggcta cgtcgagccg tccgccggcc agctggccgc
     2761 cgagacgcgc gtccagttcg agcgccatgg ctacttcgtc accgaccgcg tcgaccacca
     2821 gccgggcaag ccggtcttca accgcatcac cacgctcaag gacagctggg gcaagtaggc
     2881 ggcggcaacc tggcccttga atcgggccgg gacgacccta actgcgatcc gagggcagtc
     2941 acgccgcccc cgccgtcgta cctgaaggag tcgtgatgac cgccgcgctg aagaaccgca
     3001 tcgagaacct gatcgccatg gcccagttgc tggagcgcgt ggacgcgaac ccgacgatcg
     3061 tcggcgcgca gcagtacctg aacctcgtca atacggtcaa gggcctgctc ggcgaggacg
     3121 acctgcccga cgacgccctg cgcgcggtgc tgcgcgcctg cccggccagc gcgatgctct
     3181 acgagaacat gcactacgac cgctcgggcc tgtcgcagtc gccgctggac gcggccgtgg
     3241 cctccgagat ggcggccgcc gacctgatcg ccggactgcg cgcccgcgca cattgacacg
     3301 ttcgcgcgac gcgcggacgc aacagatggg cggggcgtcg ctgacgcgac agacagcctg
     3361 cgttcaac
//
LOCUS       DATCRO010000855         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_133497, whole genome shotgun sequence.
ACCESSION   DATCRO010000855 DATCRO010000000
VERSION     DATCRO010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133497"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VIN75_23300"
     CDS             <1..758
                     /locus_tag="VIN75_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY8977159.1"
                     /translation="TWDIGLQQRRGVGYVYSSRHTGDERAERVLRDYIGPAAEGLAPR
                     ALKLDVGYRERTWIGNCVAIGLSAGFLEPLESSGIGLIEFGAYLVGYLLPSVEDLDTA
                     AASFNQMMTARFERIVDFLKLHYAITRRTDTAFWRDNADPSSWPGSLRDKLALWRSRP
                     PHRLDFVADLEMYPSSSWQYVLYGMGYPTDLAGRHAAWPRVEDARREFAMIPQVAQRA
                     LQDLPDHRALVQAMAARAAAKPVPARPAMPVRA"
     gene            758..1849
                     /locus_tag="VIN75_23305"
     CDS             758..1849
                     /locus_tag="VIN75_23305"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8977160.1"
                     /translation="MTTSRLSTPVAGATATINDVAQQAGVSIKTVSRVMNNEAGVRPD
                     TRARVLQVMADLRYRPSQSARSLAGARSFLIGLLYFDPSAAFVSGVQQGATMRCREAG
                     YHLVVESMDNDAHDLGEQVHRMVSTLRPDGMILTPPLCDNPHVLKALHDSGTPTVLVS
                     PARQRKDMPSVRMDDVHAAEEITNLLISLGHRRIAFIRGAADQAASALREQGFAKAMK
                     AARMDVAPELVIDGDFTFESGVAATHQLLKLRHHPTAVFASNDDMALGVLAAAQRQGL
                     AIPGDLAIVGFDDSPASSLVWPPLTTMRQPTLAMARTAVDLLIGTPTVEGTPGTRTSS
                     AMQRVLPHELVVRDSTAAPARPARRTPRG"
     gene            2028..3008
                     /locus_tag="VIN75_23310"
     CDS             2028..3008
                     /locus_tag="VIN75_23310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977161.1"
                     /translation="MRLALASLRTVAALCVALVAAAHQPTMAADAASQAQPGAAPTAA
                     QYFSLVRPAAPAARPARGARTMAAVDANAPTLRSISTALFVDAMGDIPQLLVNVKAID
                     DASGAYYLTLVATGPNGQTKYGSLGPQFPVATLSGTLALRFGPHDAPGLWHVTALEVG
                     DAAGNFHDYTAQELAALGNTRFWVQNSYAKYLDNTAPVLTSATILTPSVSTAGGNSVT
                     VGVKVVASDAGNPAATGVQQAELYFCLLDGSNCFSMVQNDSVEGRTSTTLMPAVGVAS
                     PGYAPGDYYLYYVHLTDWAGNQSFAVGTEFGGTTDFSKLMVAGHKITLLP"
     gene            3119..>3570
                     /locus_tag="VIN75_23315"
     CDS             3119..>3570
                     /locus_tag="VIN75_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8977162.1"
                     /translation="MDSPETFCLPLLHADDAIVAVDKPAGLLVHRSSLDAHETRSAAE
                     LLRAQLGVPVWLLHRLDKATSGVLAFALDAQVAAAVGRAFESGAVRKRYLALVRGWPD
                     EQGEIDYPLARDPELPSAGQPRLPAVTRWRRLACFEWPFPDGRHATSR"
BASE COUNT          515 a         1370 c         1204 g          481 t
ORIGIN      
        1 ggacatggga catcggcctg cagcagcggc gcggcgtcgg ctacgtctac tccagccgcc
       61 acacgggcga cgagcgcgcg gagcgcgtgc tgcgcgacta catcggaccg gccgccgagg
      121 gcctggcgcc gcgcgcgctc aaactggacg tcggctatcg cgagcgcacg tggatcggca
      181 actgcgtggc catcggcctg tcggcgggct tcctcgagcc gctcgaatcc tcgggcatcg
      241 gcctgatcga gttcggcgcc tacctcgtgg gctacctgtt gccgtcggtc gaggatctcg
      301 acacggccgc cgcctccttc aaccagatga tgaccgcgcg cttcgagcgc atcgtcgact
      361 tcctcaagct gcactacgcc atcacgcgcc gcaccgacac cgcgttctgg cgcgacaacg
      421 ccgacccgtc gtcctggccc ggctcgctgc gcgacaagct ggcgctgtgg cgcagccgcc
      481 cgccgcaccg gctggacttc gtggccgacc tcgagatgta cccgtcctcg agctggcagt
      541 acgtgctgta cggcatgggg tatccgaccg acctggccgg ccgccacgcc gcgtggccgc
      601 gcgtggagga cgcccggcgc gagttcgcga tgatcccgca ggtcgcccag cgcgcgctgc
      661 aggacctgcc cgaccatcgc gcgctggtcc aggcgatggc ggcgcgcgcc gccgccaagc
      721 ccgtcccggc ccggcccgcg atgcccgtgc gcgcctgatg acgacgtctc gcctctcgac
      781 gccggtcgcc ggcgccaccg ccaccatcaa cgatgtcgcc cagcaggccg gcgtgtcgat
      841 caagacggtc tcgcgcgtga tgaacaacga ggccggcgtg cggcccgaca cccgcgccag
      901 ggtgctgcag gtgatggccg acctgcgcta ccggcccagc cagtcggcac gcagcctggc
      961 cggcgcgcgc tcgttcctga tcggcctgct gtacttcgac ccgagcgccg cgttcgtctc
     1021 cggcgtgcag cagggcgcga cgatgcgctg ccgcgaggcc ggctaccact tggtggtcga
     1081 gtcgatggac aacgacgcgc acgacctggg cgaacaggtg caccgcatgg tgtccacact
     1141 gcggcccgac ggcatgatcc tgacgccgcc gctgtgcgac aacccgcacg tgctcaaggc
     1201 gctgcacgac agcggcacgc cgaccgtgct ggtgtcgccg gccaggcagc gcaaggacat
     1261 gcccagcgtg cgcatggacg acgtccacgc cgccgaggag atcaccaacc tcctgatcag
     1321 cctgggccat cgccgcatcg cgttcatccg cggcgccgcc gaccaggcgg cttcggcgct
     1381 gcgcgagcag ggctttgcca aggcgatgaa ggccgcgcgc atggacgtcg cgcccgaact
     1441 ggtgatcgac ggcgacttca cgttcgaatc gggcgtcgcg gcaacgcacc agctgctcaa
     1501 gctgcgccac catcccaccg ccgtcttcgc cagcaacgac gacatggcgc tgggcgtgct
     1561 ggccgcggcg cagcgccagg gcctggcgat ccccggcgac ctggcgatcg tcggcttcga
     1621 cgactcgccc gccagcagcc tggtctggcc gccgctgacg accatgcgcc agcccacgct
     1681 ggcgatggcg cgcacggcgg tcgacctgct catcggcacc cccacggtgg aaggcacgcc
     1741 cggcacgcgc acgtcgtcgg cgatgcagcg cgtgctgccg cacgagctcg tcgtgcgcga
     1801 ctcgaccgcg gcgccggcgc gcccggcgcg gcgcacgccg cgcggctgac ctcacgttgc
     1861 gccccgccac ccgggcccgg cggactgcgt tgtcatgcgg cgcccacccc aattgggtga
     1921 tggcttgcac gcgcgcgacg ggaaaaaatg gcgccacgga gggtagggaa acggtggcca
     1981 agccgggatg cgctccgatt cgaacaaacc catcatccag gatcgccatg cgccttgctc
     2041 tcgcatctct tcgcacggtc gccgcactgt gcgtcgccct cgtcgccgcc gcgcaccagc
     2101 cgacgatggc cgcggacgcc gccagccagg cccagccggg cgccgcgccg accgcagcgc
     2161 aatatttctc gctggtgcgc cccgccgcgc ccgccgcccg cccggcgcgg ggcgcgcgca
     2221 cgatggccgc ggtcgatgcc aacgcgccga cgctgcgctc gatcagcacg gccctgttcg
     2281 tcgacgcgat gggcgacatc ccccagctcc tggtcaacgt caaggccatc gacgatgcgt
     2341 cgggcgccta ctacctcacg ctggtggcca cgggcccgaa cgggcagacg aagtacggca
     2401 gcctgggccc gcagttcccg gtcgcgacgt tgagcggcac gctggcgctg cgcttcggcc
     2461 cgcatgacgc cccgggcctg tggcacgtca cggccctgga ggtcggcgat gccgccggca
     2521 atttccacga ctacaccgcg caggaactcg ccgcgctcgg caacacgcgc ttctgggtgc
     2581 agaacagcta cgccaagtac ctggacaaca ccgcgccggt gctgacctcc gcgacgatcc
     2641 tgaccccgtc ggtgtcgacc gcgggcggca actcggtcac cgtcggcgtc aaggtcgtcg
     2701 ccagcgacgc gggcaacccc gcggccaccg gcgtgcagca ggccgagctg tacttctgcc
     2761 tgctcgacgg cagcaactgc ttcagcatgg tgcagaacga ctccgtcgag ggccgcacgt
     2821 ccaccacgct gatgccggca gtcggcgtcg cgagccccgg ctacgcgccg ggcgactact
     2881 acctgtacta cgtccacctg accgactggg cgggcaacca gagctttgcc gtcggcaccg
     2941 agttcggcgg caccaccgac ttctccaagc tgatggtggc cggccacaag atcacgctgc
     3001 tgccctgagg agggcacgcg cgcccgggtc gcaacagcgc cgggcgcgcg cgcagcggcc
     3061 atctgcgacg cccgacgcgg cgtcgaccat cgggcgaacc catcgacaat cgacgtcgat
     3121 ggattcgccc gaaacctttt gcctgccgct gctgcacgcc gacgacgcga tcgtcgcggt
     3181 cgacaagccc gcgggcctgc tggtgcaccg ctcgtcgctt gacgcgcacg agacgcgcag
     3241 cgccgccgag ctgctccggg cgcagctggg cgtccccgtc tggctcctgc accgcctcga
     3301 caaggccacc tccggcgtgc tggcgttcgc actggacgca caggtcgcgg cggcggtcgg
     3361 cagggccttc gagtcgggcg cggtgcgcaa gcgctacctg gcgctggtgc gcggctggcc
     3421 cgacgaacag ggcgagatcg actatccgct cgcgcgcgat cccgagctgc cctcggccgg
     3481 ccagccgcgg ctgcccgcgg tcacgcgctg gcgccggctc gcctgcttcg agtggccatt
     3541 ccccgacggc cgccacgcga cgagccgcta
//
LOCUS       DATCRO010000856         8289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_403382, whole genome shotgun sequence.
ACCESSION   DATCRO010000856 DATCRO010000000
VERSION     DATCRO010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403382"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIN75_23320"
     CDS             <1..221
                     /locus_tag="VIN75_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977163.1"
                     /translation="VDVRARRAGVCKVPAFVPKAGEQYEVMVDLSPARCTVTPYRLVG
                     EGGAVRREPVESQVSRISTYEADMKCFR"
     gene            complement(214..738)
                     /locus_tag="VIN75_23325"
     CDS             complement(214..738)
                     /locus_tag="VIN75_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977164.1"
                     /translation="MTTPSTPSFSLRAATLADLDAIDDIHVRSRRVTYRGQVSDHYLD
                     VTMPAASRADWTLKLPAMLAGAGQVLVAESQGEPIGFVCALAPDADRSVYVNNLHALP
                     ERKGLGIGTALLDATARWARAAGATRMHLRVLETNVAAIAFYESRGWRCAGRAQDAWA
                     GEPIVALVYAIDLT"
     gene            complement(771..1340)
                     /locus_tag="VIN75_23330"
     CDS             complement(771..1340)
                     /locus_tag="VIN75_23330"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8977165.1"
                     /translation="MIRSASSNLPARRTAGMALAQGIIDTPLGPLTALASDAGLMGLW
                     FDSQQHGPGPLAAPLDNGQRWIAQARDELERYFAGRLRKFEVALAPRGTPFQEAVWRR
                     LCAIRCGETTSYGRIARDVGSPQASRAVGAAVGRNPISIIVPCHRVIGENGTLTGYAG
                     GLHRKQALLDVEGIVLAPGSRLDAQAALP"
     gene            complement(1337..2965)
                     /locus_tag="VIN75_23335"
     CDS             complement(1337..2965)
                     /locus_tag="VIN75_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEY8977166.1"
                     /translation="MELDPDTAWKALATHDARFDGRFFVGVTSTRIYCRPVCRVRTPR
                     KENCRFYPNAALAEQAGFRPCLRCRPELAPGLSLMDSSQVLAQHAARMIDHAVRVGAA
                     VRMPELAARLGVTERHLRRVFAQAHGVSPIDYLTTQRLLQAKQLLTDTALPVTEVALA
                     CGFESLRRFNAVFAEQVRLSPTELRKAGSAAQPATGGGTGLRVRLGYRPPYDVDSMLA
                     FWDQRTLAGVEEVAGRTIRRTWSAPRAAADETGQAGRAAQPARGWIEIEFLADRHEVE
                     LRAGANLAVHSGQLVEAARHALDLDADPSHMAPLLQTLAALHPGSAMTAGLRMPGSFD
                     GFETAARIILGQQVTVAAARTLTRRLIERFGAAVETPWPGLHRCFPDAATIAAATPDS
                     IGELGIVRQRVAALQALARAVADGLPLHRGAPLAATQEALLALPGIGDWTVQLLALRV
                     LGWPDAFPATDIGLLKAMGLAQARDAPQAIALAESWRPWRSYAVIALWRALESGDKAP
                     PPAARAPISPRKRNAVATPKGIPARRKVTAKETR"
     gene            3331..4998
                     /locus_tag="VIN75_23340"
     CDS             3331..4998
                     /locus_tag="VIN75_23340"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY8977167.1"
                     /translation="MPAAPVPPPVAPANANAGRALESSTPPQPREIKKDANVLPPTEA
                     ARAPAAGDNGPTLLVKGFQVTGNTIFTSEELLALVKPWVGKQAGTDQLLDAAEAIKNR
                     YKDAGFFLTQVFVPAQEVPDGIVTLRVLEARIGETHAEVNTERVTPAIVDGYMRLLPK
                     GAAVTEQNVERPLLLLNDLPGLKVTSVLRPGASTGEADLLVKVADDGKTFGGDGYIDN
                     DGNASTGYVRVGADLVANGLLGQGESWTLGGLATEHNGVDIVRGGVTAPVGPYGTKAT
                     ASLTYLHYNVLKLTDLHADGYAAVGSLMVQHPVIRSRNFNLFAVAGGDVKDLDDRTQD
                     RLVHDERLLYIGHLGVTGDFRDERFGGSLNSFTLTLTAGDNRIQSHDALATDLGPKYG
                     HKTSGMFEHVDGDYQRLQAISETTSVLVSMRGQLAFQNLDTSEKSSLGGPHGVRAYAV
                     GDGVGDDVFQGTVELRQRVPAWTLFNAPFVLSAFIDGGRYKNWHDPVSGDDFNTGTLG
                     GYGVGLNMTSRDDFQFRLDIARRINKSDLPGSDNRKTRAWASLQKWF"
     gene            5097..>8289
                     /locus_tag="VIN75_23345"
     CDS             5097..>8289
                     /locus_tag="VIN75_23345"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY8977168.1"
                     /translation="MEKSKSSLLSSSMLCVRQMVLRVGFKFKLATARVRTPRPSRLLM
                     ALLAGCGVQAASALPQGGQVASGGVTIGTPANNALNITQTTNQGVINWQSFNVAGGEK
                     VNFQQPGATSSTLNRVVGSDPSAIFGQINANGQVFLINNSGVLFGPGAQVNAAGLVAS
                     TLQLSDSDYLAGNYVLSGAGGNVSNQGVLKAGFVALAGSQVSNTGTIVASGGTAALAA
                     GDRVTLNLIGSDLVSMSVDAPTAAAQVRAGGVVQADGGQVLISAKAADAMLGTVVNVD
                     GIVQARSIGSRNGVISLDGGNSGVVAVSGTLDASGKGAGETGGTVKVTGANVGLFDHA
                     AVDASGDTGGGVVRIGGDWHGAGADGDANASQAYVGSNATIAANAVTSGKGGNVVVWS
                     DDHTQFFGSVDARGGAAGGDGGKVETSGHALDVNGKVDASSRGGQGGNWLLDPVDVNI
                     THTGTTDITPSAGVWRSSSGNSTIVDTDLSAALTSGTNIQVQTTSSTGGTGNIAVTGA
                     VTSGLTGSQSATLSLEAIGNITFTGGSISSANGTALTVNLNAGTNNSHTAPGTNTSTI
                     TMDSASSIHLGTTGASMLTATAKGNIQLGGLTVGTGGVQVTSNGGAVTQSSALTVNGT
                     LTANTANGNVTLDTAGNNIGIYAGNAGTGTVSVHNASALELGGIHASSLNIDTSENNS
                     DVAQASGDLTISGGVTIDAGTGGVGAGSGASTIGHIATTGNFTFLQASSLVLDNIVST
                     ALLVSTGAAGSVTQSGGSTITAATTDLHAGTGGVTLHNGNSIGTYGANTTGTASFTNN
                     GGVKLGDVTASALTVDTSGGNGNVTQASSTNLNVTNGMTVTAGTGDITAGSTGSTIGH
                     VATSGHVTNLSASNLVLDNVTATAFTGTSGAAGSVSQTAGDTINATTSATLNGGSNGV
                     ALAGITTPTLAVNTNLHNGNVTQVAGTSLAVSGGATINAGTGSIAAGSTTSTIGHIGT
                     TGGATSLAATSLVLDNVSATTLTTHTGAAGGVTQTGGDTITATTTDLHAGTGGVTLTT
                     GDAIGTYGASTTGAASFTNAGGVNLGGISATSLT"
BASE COUNT         1289 a         3030 c         2922 g         1048 t
ORIGIN      
        1 aggtggacgt gcgcgcccgc cgcgccggcg tctgcaaggt gccggccttc gtccccaagg
       61 ccggcgagca gtacgaggtg atggtcgacc tgtcgcccgc gcgctgcacc gtcacgccct
      121 accggctggt gggcgagggc ggcgcggtca ggcgcgagcc ggtggagtcg caggtctcgc
      181 gcatctcgac ctacgaagcc gacatgaagt gcttcaggtg aggtcgatcg cgtagacgag
      241 cgcgacgatc ggctcgccgg cccacgcgtc ctgcgcccgg ccggcgcagc gccagccgcg
      301 cgactcgtag aaggcgatgg cggcgacgtt cgtctcgagc accctcaggt gcatgcgcgt
      361 cgccccggcc gcccgggccc agcgcgcggt cgcgtcgagc aacgccgtcc cgatgcccag
      421 ccccttgcgc tcgggcaggg catgcaggtt gttgacatag acgctgcgat cggcgtccgg
      481 cgccagggcg cagacgaagc cgatcggctc gccctgcgat tccgcgacga ggacctggcc
      541 ggcgcccgcg agcatcgccg gcagcttgag cgtccagtcg gcgcggctgg cggccggcat
      601 cgtcacgtcc aggtagtggt cggatacctg tccccggtac gtgacgcggc ggctgcggac
      661 gtggatgtcg tcgatggcgt ccagatcggc gagggtcgcc gcgcgcagcg agaaggaggg
      721 tgtcgagggc gtggtcaaga ggcgggcacg agtggctggt tccccgattt tcacggcagc
      781 gcggcctgcg cgtcgagccg gctgccggga gcgagcacga tgccttcgac gtccagcagc
      841 gcctgcttgc ggtgcagtcc gcccgcatac ccggtcaggg taccgttctc gccgatcacg
      901 cggtggcagg gcacgatgat cgagatcggg ttgcgtccca cggcggcgcc gaccgcgcgc
      961 gaggcctgcg ggctgccgac gtcgcgcgcg atgcggccgt agctggtcgt ctcgccgcag
     1021 cggatcgcgc acaggcgccg ccagacggcc tcctggaacg gcgtgccccg tggggcgagc
     1081 gctacctcga acttgcgcag ccggcccgcg aagtagcgct cgagctcgtc gcgcgcctgc
     1141 gcgatccagc gctggccgtt gtccagcggc gccgccagcg ggccggggcc gtgctgctgg
     1201 ctgtcgaacc acaggcccat caggcccgcg tcgctcgcca gcgcggtgag cggacccagc
     1261 ggggtgtcga tgatgccctg ggcgagggcc atgccggcgg tgcgccgggc gggcaggttg
     1321 gaggaggcgg atcggatcat cgtgtttcct tggcggtgac cttgcggcgg gcagggatac
     1381 ccttgggggt agcgactgca tttcgtttcc tggggcttat cggcgcccgg gcggccggcg
     1441 gcggcgcctt gtcgccggat tccaacgccc gccataacgc gatgacggcg taggagcgcc
     1501 acggccgcca gctctcggcc agcgcgatcg cctgcggcgc atcgcgtgcc tgggccaggc
     1561 ccatcgcctt gagcaggccg atgtcggttg ccgggaaggc gtcgggccag cccagcacgc
     1621 gcagcgcgag cagttgcacg gtccagtcgc cgatccccgg cagcgcgagc agcgcctcct
     1681 gggtcgcggc cagcggcgcg ccgcggtgca gcggcaggcc gtcggccacc gcgcgcgcca
     1741 gcgcctgcag cgcggccacg cgctggcgca cgatgcccag ttccccgatc gagtcggggg
     1801 tggcggcggc gatggtggcg gcatcgggaa agcagcggtg cagccccggc cacggcgtct
     1861 ccacggccgc gccgaaacgc tcgatcaggc gccgcgtcaa ggtgcgcgcg gcggccaccg
     1921 tcacctgctg gcccaggatg atgcgggcgg ccgtctcgaa gccgtcgaag ctgccgggca
     1981 tgcgcaggcc ggcggtcatc gccgagccgg ggtgcagcgc cgccagcgtc tgcagcagcg
     2041 gcgccatgtg cgacggatcg gcgtccaggt cgagtgcatg gcgcgccgcc tccaccagct
     2101 ggccgctgtg gacggcgagg ttggcgccgg cgcgcagttc gacctcgtgg cggtcggcca
     2161 ggaactcgat ctcgatccag ccgcgggccg gctgcgcggc gcggccggcc tggcccgttt
     2221 cgtcggcggc cgcgcgcggg gcgctccagg tgcggcggat cgttcggccg gcgacttcct
     2281 cgacgcccgc cagcgtgcgc tggtcccaga acgccagcat gctgtcgacg tcatacggcg
     2341 ggcggtatcc gagccgcacg cgcaggcccg tgccgccgcc tgtcgccggc tgggccgcgc
     2401 tgccggcctt gcgcagctcg gtcgggctga gccgcacctg ctcggcgaag acggcgttga
     2461 agcggcgcag gctctcgaag ccgcaggcca gcgccacctc ggtgacgggc agcgcggtgt
     2521 cggtcagcaa ctgcttggcc tgcagcaggc gctgggtcgt gaggtagtcg atcgggctga
     2581 cgccgtgggc ctgggcgaac acccggcgca ggtggcgctc ggtcacgccc aggcgggccg
     2641 ccagctcggg catgcgcacg gcggcgccca cgcgcacggc atgatcgatc atgcgtgccg
     2701 cgtgctgcgc cagcacttgc gaggagtcca tcaggctcag gcccggggcg agctcggggc
     2761 ggcagcgcag gcagggccgg aagccggcct gttcggccag cgcggcgttc gggtagaagc
     2821 ggcagttctc cttgcgcggc gtgcgcacgc ggcagacggg gcggcagtag atgcgggtcg
     2881 acgtcacgcc cacgaagaag cggccgtcga agcgggcgtc atgcgtggcc agggccttcc
     2941 aggcggtgtc gggatcgagt tccatgccgc catcataggc acgccggcga ccgcgactcg
     3001 ccgttttcgg acacatggat tcagggcctc tcgcgtgacc accgcgtggc agtcccttgc
     3061 cggcaatagg ggttcccgcc gatacccccc acagcacctg cggcgcacct tgtaaaaccc
     3121 cctggtgggg gatgcgctgc acggctgttg gcgtcagaat ccccccggga cgtggccagc
     3181 tccggacggc cacgcgagac cgacaaagcg tccccgcgcc aggcggggca gaggaaggag
     3241 agcgagttga aatctcatac ccttggcggg ttgggctgcg tcgtcgtggc tcttgcaggc
     3301 ctgaatgccg caccgtcgtt cgcacagacc gtgccagccg ctcccgtccc gccgcccgtt
     3361 gcgcccgcca atgccaacgc cggccgcgcc ctcgaatcct ccaccccgcc gcagcccagg
     3421 gaaatcaaga aggacgccaa cgtcctgccg cccaccgagg ccgcgcgcgc gccggcggcc
     3481 ggcgacaacg gcccgacgct gctggtcaag ggcttccagg tcacgggcaa cacgatcttc
     3541 accagcgagg agctgctcgc cctcgtcaag ccctgggtcg gcaagcaggc cggcaccgac
     3601 cagctgctcg atgccgccga ggccatcaag aaccgctaca aggacgccgg cttcttcctg
     3661 acgcaggtgt tcgtgcccgc gcaggaggtg cctgacggca tcgtcacgct gcgcgtgctc
     3721 gaggcccgca tcggcgagac ccatgccgag gtcaacaccg agcgcgtgac gccggcgatc
     3781 gtcgacggct acatgcgcct gctgcccaag ggcgcggccg tcaccgagca gaacgtcgag
     3841 cgcccgctgc tgctgctcaa cgacttgccc ggcctgaagg tcacctccgt gctgcgcccc
     3901 ggcgcgtcga cgggcgaggc cgacctgctc gtgaaggtgg ccgacgacgg caagaccttc
     3961 ggcggcgacg gctacatcga caacgacggc aacgcgagca ccggctacgt ccgcgtcggc
     4021 gccgacctcg tcgccaacgg cctgctgggc cagggcgagt cgtggacgct ggggggcctg
     4081 gccaccgagc acaacggcgt cgacatcgtc cgcggcggcg tcacggcgcc cgtgggcccg
     4141 tacggcacca aggccacggc cagcctcacc tacctgcact acaacgtgct caagctgacc
     4201 gacctgcacg ccgacggcta tgccgcggtc ggctcgctga tggtgcagca cccggtgatc
     4261 cgctcgcgca acttcaacct gttcgcggtc gcgggcggcg acgtcaagga cctggacgac
     4321 cgcacgcagg atcggctggt gcacgacgaa cgcctgctgt acatcggcca cctcggcgtc
     4381 accggcgact tccgcgacga gcgcttcggc ggctcgctca acagcttcac gctgacgctc
     4441 accgccggcg acaaccgcat ccagtcgcac gacgcgctcg ccaccgacct ggggcccaag
     4501 tacggccaca agacgagcgg catgttcgag cacgtcgacg gcgactacca gcgcctgcag
     4561 gccatctccg agaccaccag cgtcctcgtg tcgatgcgcg gccagctcgc gttccagaac
     4621 ctggacacct cggagaagtc ctcgctgggc ggcccgcacg gcgtgcgcgc ctacgcggtg
     4681 ggcgacggcg tcggcgacga cgtgttccag ggcaccgtgg aactgcgcca gcgcgtgccg
     4741 gcatggacgc tgttcaacgc cccgttcgtg ctcagcgcct tcatcgacgg cggccgctac
     4801 aagaactggc acgacccggt cagtggcgac gacttcaaca ccggcacgct gggcggctac
     4861 ggcgtcggcc tgaacatgac cagccgcgac gacttccagt tccggctgga catcgcgcgc
     4921 cgcatcaaca agtccgacct gccggggtcc gacaaccgca agacgcgggc gtgggcatcg
     4981 ctgcagaagt ggttctgatc tgagggggag ccccgcatcc gtcgcggggt ccgtgggatt
     5041 cgttggcgcg ccgccgggcg catcgctcgc cgcgccgtga aatgctgggg gttgtcgtgg
     5101 aaaagtccaa gtcttcgctg ttgtcgtcct cgatgctgtg cgtgcgccag atggtgctgc
     5161 gcgtcgggtt caagttcaag ctcgcgaccg cgcgcgtgcg cacgccgcgc ccgagccggc
     5221 tgctgatggc gctgctggca ggctgcggcg tgcaggccgc ctccgcgctg ccccagggcg
     5281 gccaggtggc ctccggcggc gtgacgatcg gcacgccggc gaacaacgcg ctcaacatca
     5341 cccagacgac caaccagggc gtcatcaact ggcagtcctt caacgtcgcc ggcggcgaga
     5401 aggtgaactt ccagcaaccg ggcgcgacgt ccagcacgct caatcgcgtc gtcggcagcg
     5461 accccagcgc catcttcggc cagatcaacg cgaacgggca ggtcttcctg atcaacaaca
     5521 gcggcgtgct gttcggtccg ggcgcgcagg tcaacgccgc gggcctcgtg gcctcgacgc
     5581 tgcagctgag cgacagcgac tacctcgccg gcaactacgt gctgagcggc gcgggcggca
     5641 acgtcagcaa ccagggcgtg ctcaaggccg gcttcgtggc gctggcgggc tcgcaggtgt
     5701 ccaacaccgg gaccatcgtc gccagcggtg gcaccgccgc gctcgcggcc ggcgaccgcg
     5761 tcaccctcaa cctgatcggc agcgacctgg tgtcgatgtc ggtcgacgcg cccaccgcgg
     5821 ccgcgcaggt gcgcgccggc ggcgtggtgc aggccgacgg cggccaggtg ctgatctcgg
     5881 ccaaggcggc cgacgcgatg ctgggcaccg tcgtcaacgt cgacggcatc gtgcaggcgc
     5941 gctccatcgg ctcgcgcaac ggcgtcatct cgctcgatgg cggcaactcg ggcgtggtcg
     6001 cggtcagcgg cacgctggac gccagcggca agggcgccgg cgagacgggc ggcaccgtca
     6061 aggtcaccgg tgcgaacgtc ggcctgttcg accacgcggc ggtcgacgcc tccggcgaca
     6121 caggcggcgg cgtcgtgcgc atcggcggcg actggcacgg cgcaggcgcc gacggcgacg
     6181 ccaacgcgag ccaggcctac gtcggctcca acgccacgat cgcggcaaac gcggtcacgt
     6241 cgggcaaggg cggcaacgtc gtcgtctggt ccgacgacca cacgcagttc ttcggttcgg
     6301 tcgacgcgcg cggcggcgcc gcgggcggcg acggcggcaa ggtcgagacc tcgggccatg
     6361 cgctggacgt caacggcaag gtggacgcct cgtccagggg cgggcagggc ggcaactggc
     6421 tgctcgaccc ggtcgacgtg aacatcaccc acaccggcac gaccgacatc accccctcgg
     6481 ccggcgtctg gcgcagttcg tcgggcaact ccacgatcgt cgacaccgat ctcagcgccg
     6541 ccctgaccag cggcaccaac atccaggtgc agaccacctc gagcaccggc ggcacgggca
     6601 acatcgccgt cacgggcgcg gtgacctccg gcctgaccgg cagccagtcg gccacgctct
     6661 cgctcgaggc gatcggcaac atcacgttca ccggcggctc gatcagctcg gccaacggca
     6721 cggccctgac ggtcaacctc aacgcgggca ccaacaacag ccataccgcg ccgggcacca
     6781 acaccagcac catcacgatg gactcggcct cgtccatcca cctgggcacg accggcgcgt
     6841 ccatgctgac ggccacggcc aagggcaaca tccagctcgg cggcctgacg gtgggcaccg
     6901 gcggcgtgca ggtcacgtcc aacggcggcg cggtcacgca gtccagcgcg ctgacggtca
     6961 acggcacgct gaccgccaac accgccaacg gcaacgtcac gctcgacacc gcgggcaaca
     7021 acatcggcat ctacgcgggc aatgccggca ccggcaccgt gtcggtccac aacgcctccg
     7081 cgctggagct cggcggcatc cacgcgagca gcctgaacat cgacaccagc gagaacaact
     7141 cggatgtcgc gcaggcctcg ggcgacctca cgatctcggg cggcgtcacg atcgacgccg
     7201 gcaccggcgg cgtcggcgcg ggcagcggcg ccagcacgat cggccacatc gccaccaccg
     7261 gcaacttcac gttcctgcag gcctcctcgc tcgtcctgga caacatcgtc tcgacggcgc
     7321 tgctggtcag caccggcgcg gcgggcagcg tcacgcagag cggcggcagc accatcacgg
     7381 cggccaccac cgacctgcac gcaggcaccg gcggcgtcac gctgcacaac ggcaactcga
     7441 tcggcacgta cggcgccaac accaccggca ccgccagctt caccaacaac ggcggcgtga
     7501 agctgggcga cgtgacggcc tcggcgctga ccgtcgacac cagcggcggc aacggtaacg
     7561 tcacgcaggc gtccagcacg aacctgaacg tcaccaacgg catgacggtg acggccggta
     7621 ccggcgacat caccgccggc agcacgggca gcacgatcgg ccacgtcgcc acgagcggcc
     7681 acgtcaccaa cctgagcgcc agcaacctgg tgctggacaa cgtcacggcg accgcgttca
     7741 cgggcaccag cggcgccgcg ggcagcgtct cgcagacggc cggcgacacg atcaacgcga
     7801 ccaccagtgc caccctcaat ggcggcagca acggcgtcgc gctggccggc atcacgacgc
     7861 cgacgctggc ggtcaacacc aacctgcaca acggcaacgt gacacaggtc gcgggcacca
     7921 gcctggcggt cagcggcggc gccacgatca acgcgggcac cggcagcatc gcggccggca
     7981 gcaccaccag cacgatcggc cacatcggca cgacgggcgg cgccaccagc ctggcggcca
     8041 ccagcctcgt gctggacaac gtgtcggcca cgacgctgac cacgcacacc ggcgcggccg
     8101 gcggcgtcac gcagacgggc ggcgacacga tcaccgccac cacgaccgac ctgcacgcgg
     8161 gcaccggcgg cgtcacgctg accaccggcg acgcgatcgg cacctacggc gccagcacca
     8221 cgggcgcggc cagcttcacc aacgccggcg gcgtcaacct gggcggcatc tcggccacgt
     8281 cgctgaccg
//
LOCUS       DATCRO010000857         5634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_77063, whole genome shotgun sequence.
ACCESSION   DATCRO010000857 DATCRO010000000
VERSION     DATCRO010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77063"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VIN75_23350"
     CDS             <1..2076
                     /locus_tag="VIN75_23350"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8977169.1"
                     /translation="PLPPPPPPRSFDKIAPAPVRTPPPSAAEHRASRPIPLTMMTRDD
                     EHDFARAFASDDEDTVVASGHHSASPEADTVIAVAAPMSRAHGATPSLRLLGLLMTVL
                     LALWIARLGAAPLFDVDEGAFAEATRELAVGHDWGSTTLNGVDRFDKPILVYWFQAIS
                     LKTFGLNEFAVRLPSALCAFFWCLAIGQFAWRRFGPSVAIAAAGILATSLGPMLIGRA
                     ATADSLLNLLLTLAALDLWRHLENGQDTPRRRAFAWIGLGLLAKGPVALLIPAAAAGI
                     WLFSKRDRSRLQRLVSDPKAWGLMVLIAAPWYVYAIARHGMAFIDGFLLHHNVERFTG
                     SLEGHAGSLFYYLWMLPVEMMPWTPLLVPLAMHLKARWNEPIGRFLLGWLGFVLVFFS
                     LSGTKLPHYVLYGFTPLMLLAAQELVGAKLNLRLALCVTIVAVVALGAASPWLAHSLS
                     ETTSDPLYKALLSTAPPAPWVWVAVPVGLLVFALTIWVPNVRNYLSEADGAIAAGLLA
                     AGWLVLAVLPWWGQAIQDPIRTLARTVPIAQMTLLDRQRVVQWRLHQPSIAFYLNQTA
                     ERADPKGGDWAFTRIDRVKPDELAKLEVVQQVRGYALVRPAPAASEPLSRIGAERDAA
                     QAASEAASAQALAIAAAKAASEAAEAASAAAAAVAAKLNPASAPAAASAVAPAPAPAP
                     AALPAKKGK"
     gene            2073..2828
                     /locus_tag="VIN75_23355"
     CDS             2073..2828
                     /locus_tag="VIN75_23355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8977170.1"
                     /translation="MSDAPNARAARRGWRARNKAGWIGLTAALLLAWIFTAWPQLDLW
                     ASGLFHDKAGAFIGDQFAFVRLLYHGIPWSGKFYALVGLVVIVLSIWRPHPIGGRWSR
                     RLAALAWVSLLGSGLLVNAGLKEYWGRARPVQVTEFGGTAHFSPALTPTNQCEHNCSF
                     VSGHATSGYILMAVGLMGSVATRRRWLWIGLAWGVIVSLARIAEGGHFLSDTLFAGLA
                     VWASGWLIREAWLRHVALRWRRLRRRREALAKS"
     gene            complement(3060..3752)
                     /locus_tag="VIN75_23360"
     CDS             complement(3060..3752)
                     /locus_tag="VIN75_23360"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY8977171.1"
                     /translation="MHSTTHAPRRSRPATRATLAAAVLALATAAPAHAETGTGTALKN
                     TAIAGTIATWTVAALKGAPFFDCLGRGTPGNDGWGARAFAAKDAELSTESIGAERHEC
                     KLTEAGPFSLDMSPLLSASAWQGKDDSLYSHSAWDVAFVPMMHWRYPVSGGARLDLEF
                     GIGPAYLSETNIGNRRKGSNFQFSDHFGVGVSSADGHWRAGFAFRHISNLSIKTPNNA
                     VDFKGIAIEYTP"
     gene            3875..4759
                     /locus_tag="VIN75_23365"
     CDS             3875..4759
                     /locus_tag="VIN75_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8977172.1"
                     /translation="MFLVAWISRWPLGLLHAVGGLLGWLVWLCSPTYRRRLRTNAGLA
                     GVAARDRRRSIAEAGRMVAETPWLWLTATNDDLLRKVQWRGAEALEGYIAERRPLVIL
                     TPHMGSFEVAARAYTARWGGVQPVTVLYRPARQRWLREFQERARRRPTMPTAPASLAG
                     VRQMLRALKRRETVGLLPDQVPPESQGVWAPFFGQPAYTMTLAARLSQQAGAHCLVLR
                     CERLPRGAGYVIHASELVRPLPAGTDAASLVEAATIVNETMEREILADPGQYLWGYNR
                     YKAPRAAETAESRPEGTE"
     gene            4756..>5634
                     /locus_tag="VIN75_23370"
     CDS             4756..>5634
                     /locus_tag="VIN75_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEY8977173.1"
                     /translation="MNVVARLVLGFLWLLSWLPLAMQAAIGRGLGRLLWLLAGSRRRI
                     ALRNLELTMPDKPPEERRAIARENFQWIARSILERGLLWHAPPERLRRLIRVEGDVGF
                     AERSTGAVMWLVPHFVALDVAGSASQLFQNRPVGSIYQRQSIEAFDVAIRKGRLRFGN
                     GNVFSRHEKALPLIRAIRRGYVFFNLPDMDFGPQEAVFVPFMGVPAATLLAPSKMAKS
                     LDMTVQPVVAEILPGGQGYRVRFLEPWTDFPTDDPVADAARMNEWIASEVRRNPAQYY
                     WVHKRFKTRPPGEASLY"
BASE COUNT          720 a         2021 c         2035 g          858 t
ORIGIN      
        1 ccgctgcccc cgccgccgcc cccgcgttcg ttcgacaaga tcgcgccggc cccggtgcgc
       61 acgccgccgc cgtcggccgc ggaacatcgc gcctcgcgcc cgatcccgct gacgatgatg
      121 acgcgcgacg acgagcacga cttcgcacgc gccttcgcgt ccgacgacga ggacacggtg
      181 gtcgccagcg gccaccactc ggcctcgccc gaggccgaca ccgtcatcgc cgtggccgcg
      241 ccgatgagcc gcgcgcacgg cgcgacgccg tcgctgcgcc tgctgggcct gctgatgacg
      301 gtgctgctgg cgctgtggat cgcgcgcctg ggcgcggcgc cgctgttcga cgtcgacgag
      361 ggcgcgttcg ccgaggccac gcgcgagctg gcggtgggcc acgactgggg cagcaccacg
      421 ctcaacggcg tcgaccgctt cgacaagccc atcctggtct actggttcca ggcgatcagc
      481 ctgaagacct tcggcctgaa cgagttcgcg gtgcgcctgc cgtccgcgct gtgcgcgttc
      541 ttctggtgcc tggccatcgg ccagttcgcg tggcggcgct tcgggccgtc ggtggccatc
      601 gcggctgccg gcatcctggc caccagcctc gggccgatgc tgatcggccg cgcggccacc
      661 gccgactcgc tgctgaacct gctgctcacc ctggcggcgc tcgacctctg gcgccatctc
      721 gagaacggcc aggacacgcc gcgccgccgc gccttcgcgt ggatcggcct ggggctgctg
      781 gccaaggggc cggtggcact gctgatcccg gccgcggccg ccggcatctg gctgttctcc
      841 aagcgcgacc gctcgcgtct gcagcgcctg gtgagcgatc cgaaggcgtg ggggctgatg
      901 gtgctcatcg ccgcgccctg gtacgtctac gcgatcgcgc gccacggcat ggcgttcatc
      961 gacggcttcc tgctgcacca caacgtcgag cgcttcaccg gctcgctgga aggccacgcc
     1021 ggcagcctgt tctactacct gtggatgctg ccggtcgaga tgatgccgtg gacgccgctg
     1081 ctggtgccgc tggccatgca tctgaaggcg cgctggaacg agccgatcgg ccgcttcctg
     1141 ctcgggtggc tgggcttcgt cctggtgttc ttctcgctgt cgggcaccaa gctgccgcac
     1201 tacgtgctgt acggcttcac gccgctgatg ctgctggcgg cgcaggaact ggtgggcgcc
     1261 aagctcaatc tgcggctggc cctgtgcgtg acgatcgtgg cggtggtggc gctcggcgcg
     1321 gccagcccat ggctcgcgca ttcgctgtcc gagaccacga gcgacccgct gtacaaggcg
     1381 ctgctgtcga ccgcgccgcc cgcgccgtgg gtatgggtgg cggtgcccgt gggcctgctc
     1441 gtgttcgcgc tcacgatctg ggtgcccaac gtgcgcaact acctgagcga ggccgacggc
     1501 gcgatcgccg ccggcctgct cgcggccggc tggctggtgc tggcagtcct gccctggtgg
     1561 ggccaggcca tccaggatcc gatccgcacg ctcgcgcgca ccgtgcccat cgcgcagatg
     1621 acgctgctcg atcgccagcg cgtcgtgcag tggcgcctgc accagcccag catcgcgttc
     1681 tacctgaacc agacggccga gcgcgccgac cccaagggcg gcgactgggc gttcacgcgc
     1741 atcgaccgcg tcaagccgga cgagctcgcc aagctggagg tcgtgcagca ggtgcgcggc
     1801 tatgcgctgg tgcgcccggc gccggcggcg tccgagccgc tgtcgcgcat cggcgccgag
     1861 cgcgacgcgg cgcaggcggc gagcgaggcc gcatcggccc aggcgctggc catcgcggcg
     1921 gccaaggccg cctccgaggc cgccgaagcc gccagcgcgg ccgccgcggc ggtcgccgcc
     1981 aagctgaacc cggcgtccgc gccggccgcg gcctccgcgg tcgcgcccgc tcccgcgcct
     2041 gcaccagccg cgctgccggc caagaagggc aagtgagcga cgccccgaac gcccgcgcgg
     2101 cgcgccgtgg ctggcgcgcc cgcaacaagg ccggctggat cggcctgacg gcggcgctgc
     2161 tgctggcctg gatcttcacg gcatggccgc agctggacct gtgggccagt ggcctgttcc
     2221 acgacaaggc cggcgccttc atcggcgacc agttcgcgtt cgtgcgcctg ctgtaccacg
     2281 gcatcccgtg gtcggggaag ttctacgcgc tggtgggcct ggtcgtgatc gtgctgtcga
     2341 tctggcgccc gcacccgatc ggcggacgct ggtcgcgccg gctcgcggcc ctggcctggg
     2401 tcagcctcct cggctcgggt ctgctggtca acgccggcct gaaggaatac tggggccgcg
     2461 cgcggcccgt gcaggtcacc gagttcggcg gcacggcgca tttctcgccg gcgctcacgc
     2521 ccaccaacca atgcgagcac aactgctcgt tcgtcagcgg ccacgccacg tcgggctaca
     2581 tcctgatggc cgtgggcctg atgggcagcg tggccacgcg ccggcgctgg ctgtggatcg
     2641 gcctcgcctg gggcgtcatc gtgagcctgg cccgcatcgc cgagggcggc cacttcctca
     2701 gcgacaccct gttcgccggc ctggcggtct gggccagcgg ctggctcatc cgcgaggcct
     2761 ggctgcgcca cgtcgcactg cgatggcgac gcctgcggcg gcggcgcgag gcgctggcaa
     2821 agagctgaat ccggtcttcg tcgacttctg tcggggtcga cttctgtcgg ggtcgacttc
     2881 tgtcggggtc aggtcttgaa tgttgcgggg tcaggtcttg agtgaacgat tcaagacctg
     2941 accccgcaac attcaagacc tgaccccgcc atctgacacg gcagcgattc aagacctgac
     3001 accggcatga aacgaaaagg cgacccacgg gccgcctttt cattcgcgcg agcggccgat
     3061 cagggcgtgt actcgatcgc gatgcccttg aagtcgaccg cgttgttggg cgtcttgatc
     3121 gacaggttgc tgatgtggcg gaacgcgaag ccggcgcgcc agtggccgtc ggcgctgctg
     3181 acgcccacgc cgaagtggtc ggagaactgg aagttgctgc ccttccggcg gttgccgatg
     3241 ttggtctcgc tcaggtaggc cgggccgatg ccgaactcga ggtccaggcg cgcgccgccg
     3301 gacaccgggt agcgccagtg catcatcggc acgaaggcga cgtcccacgc gctgtgcgaa
     3361 tacaggctgt cgtccttgcc ttgccaggcg ctcgccgaga gcagcggcga catgtccagg
     3421 gagaacgggc cggcctctgt cagcttgcat tcgtggcgct cggcgccgat cgactcggtc
     3481 gacagctcgg cgtccttggc cgcgaacgcg cgggcgcccc agccgtcgtt gcccggcgtg
     3541 ccgcggccca ggcagtcgaa gaacggcgcg cccttgagcg cggcgacggt ccaggtggcg
     3601 atggtgcccg cgatggcggt gttcttcagc gcggtgccgg tgccggtctc ggcgtgcgcc
     3661 ggggcggcgg tggcgagcgc cagcacggcg gcggcgaggg tcgcgcgcgt ggccgggcgc
     3721 gagcggcggg gggcgtgggt ggtcgagtgc atccgcagac tgtagcctgc ccgcgggata
     3781 gccctgtaac cggaccggac gcccgtcggg agcgcgcgac agcacggccg cagcgaaagg
     3841 gataatcgcc gaccacctcg tcacacgcca ctggatgttc ctcgttgcct ggatctcgcg
     3901 ctggccgctg ggcctgctgc atgccgtcgg cggcctgctg ggctggctcg tctggctgtg
     3961 ctcgcccacg taccgccgcc ggctgcgcac gaacgccggg ctggcgggcg tggccgcgcg
     4021 cgaccggcgg cgctcgatcg ccgaggccgg gcggatggtg gccgagacgc cgtggctgtg
     4081 gctgacggcg accaatgacg acctgctgcg caaggtgcag tggcgcggcg ccgaggcgct
     4141 ggagggctac atcgcggagc gccggccgct ggtgatcctg acgccgcaca tgggcagctt
     4201 cgaggtcgcc gcgcgggcct acacggcgcg ttggggcggc gtccagccgg tgacggtcct
     4261 gtaccgcccg gcccgccagc gttggctgcg cgagttccag gaacgggcgc gccggcgtcc
     4321 cacgatgcct accgcgcccg ccagcctggc cggcgtgcgc cagatgctgc gcgcactcaa
     4381 gcgacgcgaa acggtgggcc tgctgcccga ccaggtgccg cccgaaagcc agggcgtgtg
     4441 ggcgccgttc ttcggccagc cggcctacac catgacgctg gccgcgcgcc tgtcgcaaca
     4501 ggccggcgcg cactgcctgg tgctgcgctg cgaacgactg ccgcgcggcg ccggctacgt
     4561 catccacgcc agcgagctgg tgcggccgct gcccgcgggc accgacgcgg cgtcgctggt
     4621 cgaggccgcc accatcgtca acgagaccat ggaacgcgag atcctggccg accccggcca
     4681 atacctgtgg ggctacaacc gctacaaggc cccgcgcgcg gccgagacgg cggagtcccg
     4741 ccccgaagga accgaatgaa cgtcgtcgct cgcctggtgc tgggcttcct gtggctgctc
     4801 agctggctgc ctttggcgat gcaggccgcc atcgggcggg gcctgggccg gctgctgtgg
     4861 ctgctggccg gctcgcgccg ccggatcgcg ttgcgcaacc tcgagctgac gatgcccgac
     4921 aagccgcccg aggaacgccg cgccatcgcg cgcgagaact tccagtggat cgcgcgcagc
     4981 atcctggagc gcggcctgct ctggcatgcg ccgcccgagc ggctgcggcg gctgatccgc
     5041 gtcgagggcg acgtgggctt tgccgagcgc tcgaccggcg cggtgatgtg gctggtgccg
     5101 cacttcgtgg cgctggacgt ggcgggctcg gcctcgcagc tgttccagaa ccgcccggtc
     5161 ggctcgatct accagcgcca gagcatcgag gccttcgacg ttgccatccg caaggggcgc
     5221 ctgcgctttg gcaacggcaa cgtgttctcg cgccacgaga aggcgctgcc gctgatccgc
     5281 gcgatccgcc gcggctacgt tttcttcaac ctgcccgaca tggacttcgg cccgcaggag
     5341 gccgtgttcg tgcccttcat gggcgtgccc gcggcgacgc tgctggcgcc gtcgaagatg
     5401 gccaaatcgc tcgacatgac cgtgcagccc gtggtcgccg agatcctgcc gggcggccag
     5461 ggctaccgcg tgcgcttcct cgagccgtgg acggacttcc ccaccgacga cccggtggcc
     5521 gacgcggcgc gcatgaacga atggatcgcc tcggaagtgc gacgcaaccc ggcgcagtac
     5581 tactgggtgc acaagcgctt caagacgcgc ccgccgggcg aggcgagcct gtac
//
LOCUS       DATCRO010000858         7327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_357975, whole genome shotgun sequence.
ACCESSION   DATCRO010000858 DATCRO010000000
VERSION     DATCRO010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357975"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VIN75_23375"
     CDS             <1..586
                     /locus_tag="VIN75_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977174.1"
                     /translation="EHPAPEPSLGPEAFVARCIECIAAGGGAADITRLVRLALASQRD
                     HPEAWGHEELLHAADDLLIVDLVLPPFGTSAIHEHGTWAVIGVSQGCEVDELFVERAG
                     MLERSARHELRSGDTLVLAPDCIHFIGNPSARPARGIHVYGRHLGKVERRMWDPSTAV
                     PRPMDFALFERWESALTAASASAGAIVPPAIRGA"
     gene            complement(649..957)
                     /locus_tag="VIN75_23380"
     CDS             complement(649..957)
                     /locus_tag="VIN75_23380"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977175.1"
                     /translation="MTADAVFMNPGSAAIGPAEFPVNFGRAQRQFQVRCVSDPKDVAI
                     SGDLAVVCSRDALSLMPREGGAALEMAGDRLTVYRRGLDGRWRLARDAHTLSPVMARQ
                     "
     gene            complement(1124..1705)
                     /locus_tag="VIN75_23385"
     CDS             complement(1124..1705)
                     /locus_tag="VIN75_23385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8977176.1"
                     /translation="MNRQTWFVTGAASLLGADIVRHALAAGHRVVAVDPDPLAALQRL
                     ADVAGERLLLLPLDEIDAREVGRVVQAVVDRFGGIDVLVHGAAACSPRSPVDAPTPAH
                     DFSASVRSLFNITRGVLEAMREQGAGRIHHLVPPPELRRGPALFSIAGFCEAVAADVA
                     PFGIEVTAVPSHEAMALLRAASGRDRGELLEQA"
     gene            2126..3052
                     /locus_tag="VIN75_23390"
     CDS             2126..3052
                     /locus_tag="VIN75_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8977177.1"
                     /translation="MAHDDDAALHAEMTALVAELAPDEGMTHSLLEGVAFGRSNKSLP
                     RTPVLHDPCVVIVCSGRKRGYFGDQVIEYGAHQFMVASVPMPFEGETFASPRDPLLVI
                     KVKLDLAVIADLALAIDPAFEPVPGAESVATAPVDAPLADSVIRLLRALRSPIETRLF
                     GASLVREVCYRVLTGPKGPALRAALAQRGHFGQIAKALHRIHMDYHQRLDVETLAQEV
                     NLSAASFHAHFKTVTATSPIQYLKATRLHKARLLMIQDGVSASRASSRVGYESASQFS
                     REFKRLFGRTPVEEQRLVHAAYAPAHEGRDRP"
     gene            complement(3069..4001)
                     /locus_tag="VIN75_23395"
     CDS             complement(3069..4001)
                     /locus_tag="VIN75_23395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY8977178.1"
                     /translation="MFVVAGEALMDVFTGATTPTGIVLDARIGGSPLNVAFGFARMGR
                     PVAFLGGISSGELGGRLLDALRAEGVSLDAVHRSGAPTTISLVGVDAKGVPEYAFYGT
                     GAADRTLPLAALDRMPADARLLHVGSYTMVVGETAATQRVLVERVHGKMVVSYDPNLR
                     LNVEPSLQAWRDTLDWMLPRTDVLKLSDEDLGLLYPGIDPAAFAADCLGKGAGLVALT
                     RGGKGAFAWHASGVVEVPPVHVDVIDTVGAGDTFQSALLTRLDELGVLTPERVRGMDA
                     ETLLDAMRFAAQAAAITCSRRGADLPRRAEMGIR"
     gene            complement(4033..5292)
                     /locus_tag="VIN75_23400"
     CDS             complement(4033..5292)
                     /locus_tag="VIN75_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8977179.1"
                     /translation="MTTHDSRSSTPPRLAVIGGGPVGLSLALLMRQRCPAWSVSVFDT
                     RDAAKDISGDPRTLALSLGSVQVLERLRAWPAGQAAPIRQVHVSQAPPTLAMPFGEPV
                     VHIRADELGVPLLGAVLSYGTLLAPLQKAFLDACATTDGALGSRDAGLHARFGTAVAG
                     VKTDGDGAVVTPESGVAERFDLAVIAEGGVFAEQSRKAVTHDYRQTAWVGSVTLRGAT
                     PGLAMERFTRDGPLALLPLPSPPGSDPKTARAALVWCVDSTDDPVAPLTDAQRLAVLA
                     TRLPDAVGTPVAISPLKSFALGLNAEASLVHGHVVRIGNAAQTLHPVAGQGLNLGLRD
                     AFLLVDRLADGVHASDAGVARALRGLAFARAPDRWSVIGATDFLARSFTWHAPGLAAA
                     RGLGLAALQAFPPAKRAFARQMMFGWR"
     gene            5382..6359
                     /locus_tag="VIN75_23405"
     CDS             5382..6359
                     /locus_tag="VIN75_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8977180.1"
                     /translation="MQYRRLGRAGLQVSELSLGSWVTYANQVDVDAAVEMMAAAYDAG
                     VNFFDNAEAYAGGKSEEVMGAALKKLGWARLNYTVSTKFFWGLPDAVTVNRKNTLNRK
                     YLMQAIDGSLQRFGLDHLDLVYCHRADPNTPIEETVHAMSDMITQGKALYWGTSEWSA
                     SDIRAAWEIAERHHLHKPVVEQPQYHLFHRQKVEQEFARLYDDIGLGLTTWSPLASGL
                     LTGKYKSGIPGGSRGAMQNMSFLHDQLTDQAKNDAVARLEPIARDLGATTAQLAIAWV
                     AKNPRVSTVITGASSVKQLKDNLKASEIVAKLTPDVLARIDEITKPLAA"
     gene            complement(6802..>7327)
                     /locus_tag="VIN75_23410"
     CDS             complement(6802..>7327)
                     /locus_tag="VIN75_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8977181.1"
                     /translation="AWLEEQFPAALGDGARYGLAITYSLEGRDHGGALETAGGIAKAL
                     PLLGEAFWVVSGDVFVPDFRFDPADGQAFADSGALGQLWMVDNAPHHPRGDFGITADG
                     LASRDATPKFTWASIGLFRAALFADVPVGTKLALRPKLEFALDQRRLRAKKYDGRWTD
                     VGTMERLAALNAGD"
BASE COUNT         1075 a         2634 c         2645 g          973 t
ORIGIN      
        1 agagcacccg gcgcccgagc ccagcctggg acccgaggcc ttcgtcgcgc gctgcatcga
       61 gtgcatcgcg gccggcgggg gcgccgcgga catcacccgg ctggtgcggc tcgccctcgc
      121 ctcgcaacgc gatcatcccg aggcctgggg ccacgaggaa ctcctgcatg ccgcggacga
      181 cctgctgatc gtcgacctcg tgctgccgcc gttcggcacc agcgccatcc atgaacacgg
      241 cacctgggcc gtcatcggcg tctcgcaggg ctgcgaggtg gacgagctgt tcgtcgagcg
      301 cgccggcatg ctcgaacgca gcgcgcgcca cgagctgcga tcgggcgaca cgctggtgct
      361 ggcgcccgac tgcatccact tcatcggcaa cccgtcggcg cggccggcgc gcggcatcca
      421 cgtctacggc cgccacctgg gcaaggtcga acgccgcatg tgggatccgt cgacggccgt
      481 gccccggccc atggacttcg cgctgttcga gcggtgggag tccgcgctca ccgcggccag
      541 cgcgagcgcc ggcgccatcg tgccgcccgc gatccgcggc gcgtgacggc cacatggcgt
      601 cggggacctc ctggacggcg catccagaaa tcctccccga cacgcccgtc actgccgcgc
      661 catcaccggc gacaacgtgt gcgcgtcccg cgccagccgc cagcggccgt ccagtccgcg
      721 ccggtagacc gtcagccggt cgccggccat ctcgagggcc gcgccgccct cgcgcggcat
      781 cagcgacagc gcgtcgcggc tgcagaccac ggcgaggtcg cccgagatcg cgacgtcctt
      841 cgggtcgctg acgcagcgca cctggaactg gcgctgcgca cggccgaagt tgacgggaaa
      901 ctcggccggc ccgatggcgg cgcttcccgg gttcatgaac acggcgtcgg ccgtcatcag
      961 ggccctgcaa ctcggccagg tcgccggcgt tgacggcggc gatccaggtc gcgtgcgcct
     1021 cgcggatggc ttgttcgtcc gaagtcatgg cagtgctcgc gtggtgtcga cgacgcgatt
     1081 gtgcgagacg ccggcctcgc caggcccagg acgtgagctt ggctcaggct tgctcgagca
     1141 gctccccgcg gtcgcgcccg gaggccgcgc gcagcagcgc catcgcctcg tgcgacggca
     1201 cggccgtcac ctcgatgccg aacggcgcga cgtccgcggc gacggcctcg cagaagccgg
     1261 cgatcgagaa cagcgccggc ccacgccgga gctcgggcgg tggcacgagg tggtggatgc
     1321 ggccggcgcc ctgttcgcgc atcgcctcca gcacgccgcg cgtgatgttg aacagcgagc
     1381 gcaccgaggc gctgaagtcg tgcgcgggcg tgggcgcgtc gacggggctg cgcgggctgc
     1441 aggcggccgc gccatgcacc aggacgtcga tgccgccgaa gcgatcgacg acggcctgca
     1501 cgacgcggcc gacctcgcgg gcgtcgatct cgtccagcgg cagcaggagc agccgctcgc
     1561 ccgcgacgtc cgcaagccgc tgcagcgcgg ccagcgggtc ggggtcgacc gcgacgacgc
     1621 gatggccggc ggcgagcgcg tggcggacga tgtcggcgcc caggagcgac gccgcgccgg
     1681 tcacgaacca agtctgtcga ttcatcatct tctcctcgag aggggtggtc atgccgtcgg
     1741 ctcgcgctgc acggagcgcg ccgaaggcgt cggggttcga atggggtcct ggcgtgacgc
     1801 gtcgatgtcg tcacggcccg tggaggacgg gcgcccgcgt ggcttgccgt gacccgggct
     1861 cgccgcgggc ggggacggcg cgtcgtctct gcgcgggcct cgcacggggc tcgctggcag
     1921 ggcagccggg gaggccgctc gcgccggtcc ctgtcgaggc cgggtgaagg aagtttagga
     1981 aatggcgggc gccgggagaa gtcctgaaac tccagactta ttgcctaaac ctccaaactt
     2041 gatccgaata tgaccgtttc gtttctcatt cctggcctgt tcctttagag tctcgtcatc
     2101 gatcgatgac cgcggaggat gcgccatggc gcacgacgat gacgccgccc tgcatgcgga
     2161 gatgacggcc ctggtggccg agctggcgcc cgacgagggc atgacgcact cgctgctgga
     2221 aggcgtggcc ttcggccgtt ccaacaagtc gctgccgcgc acgccggtgc tgcacgaccc
     2281 gtgcgtcgtc atcgtctgct cgggccgcaa gcggggttac ttcggcgacc aggtgatcga
     2341 gtacggcgcg caccagttca tggtggcctc ggtgccgatg cccttcgaag gcgagacctt
     2401 cgcatccccg cgggatccgc tgctggtcat caaggtcaag ctggacctgg ccgtcatcgc
     2461 cgacctcgcg ctggccatcg acccggcgtt cgagcccgtg cctggcgccg agagcgtcgc
     2521 caccgcgccc gtcgacgcgc cgctggccga ctcggtgatc cgcctgctgc gcgcgctgcg
     2581 ctcgccgatc gagacccggc tgttcggcgc gagcctggtg cgcgaggtct gctaccgcgt
     2641 gctgaccggc cccaagggcc cggcgctgcg cgcggcgctc gcccagcgcg gccacttcgg
     2701 ccagatcgcc aaggcgctgc atcgcatcca catggactac caccagcggc tggacgtcga
     2761 gacgctggcg caggaggtca acctcagcgc ggcgtcgttc cacgcgcact tcaagacggt
     2821 gacggcgacc tcgccgatcc agtacctgaa ggcgacccgc ctgcacaagg cgcggctgct
     2881 gatgatccag gacggcgtga gcgcgagccg cgcgtccagc cgcgtcggct acgagagcgc
     2941 gtcgcagttc agtcgcgagt tcaagcgcct gttcggccgc acgccggtgg aggagcagcg
     3001 gctggtgcac gcggcgtacg cgccggcgca cgaggggcgc gaccggccct gagcgagccg
     3061 cctgacgctc agcggatgcc catctccgcg cgccgcggca ggtccgcgcc gcgccgcgag
     3121 caggtgatcg ccgccgcctg ggcggcaaag cgcatcgcgt ccagcagcgt ctcggcgtcc
     3181 atgccgcgca cgcgttcggg cgtcagcacg cccagctcgt ccaggcgcgt gaggagcgcg
     3241 gactggaacg tgtcgcccgc gccgaccgtg tcgatcacgt cgacatgcac cggcggcacc
     3301 tcgacgacgc cgctggcgtg ccacgcgaac gcgcccttgc cgccgcgcgt gagcgccacc
     3361 aggccggcgc ccttgccgag gcagtcggcg gcgaaggcgg cggggtcgat gccggggtac
     3421 agcaggccca ggtcctcgtc gctcagcttg aggacgtcgg tgcgcggcag catccagtcg
     3481 agcgtgtcgc gccacgcctg caggctgggc tccacgttca ggcgcaggtt cgggtcgtag
     3541 ctgaccacca tcttgccgtg cacgcgttcg accagcacgc gctgcgtggc cgccgtctcg
     3601 cccaccacca tcgtgtacga gccgacgtgc agcaggcgcg cgtcggccgg catgcgatcg
     3661 agcgcggcca gcggcagcgt gcggtccgcg gcgccggtgc cgtagaacgc gtactcgggc
     3721 acgcccttgg cgtcgacgcc gaccaggctg atcgtggtgg gcgcgccgct gcggtggacg
     3781 gcgtccagcg acacgccctc ggcgcgcagc gcgtccagca gccggccgcc cagctcgccg
     3841 ctggagatgc cgcccaggaa ggcgaccggc cggcccatgc gcgcgaagcc gaacgcgacg
     3901 ttcagcggcg agccgccgat gcgcgcgtcg agcacgatgc ccgtgggcgt cgtcgcgccg
     3961 gtgaacacat ccatcagggc ttcgcccgcg accacgaaca tggctgtctc tctctgggga
     4021 taactgggaa gttcagcgcc agccgaacat catctgacgc gcgaacgcgc gcttggcggg
     4081 ggggaaggcc tgcagcgccg cgaggcccag gccgcgcgcg gcggccagcc ccggcgcgtg
     4141 ccaggtgaag ctgcgggcaa ggaagtcggt ggccccgatg acgctccagc ggtcaggcgc
     4201 gcgcgcgaac gcgagtccgc gcagcgcgcg ggcgacgccc gcgtcgctgg cgtggacgcc
     4261 gtcggccagc cggtcgacca gcaggaaagc gtcgcgcagc ccgaggttca ggccctggcc
     4321 ggcgacggga tgcagcgtct gcgccgcgtt gccgatgcgc acgacgtggc cgtggacgag
     4381 gctggcctcg gcattgaggc cgagggcgaa cgacttcagc ggcgagatcg ccaccggcgt
     4441 gccgaccgcg tcgggcaggc gcgtggcgag caccgccagg cgctgcgcgt cggtcagcgg
     4501 cgcgaccggg tcgtcggtcg aatcgacgca ccacaccagc gcggcgcgcg cggtcttcgg
     4561 gtcgctgccg ggcggcgacg gcagcggcag cagcgccagc gggccgtcgc gcgtgaagcg
     4621 ctccatcgcc aggcccggcg tcgcgccccg cagcgtgacg ctgcccaccc aggcggtctg
     4681 ccggtagtcg tgcgtgacgg ccttgcgcga ctgctccgcg aacacgccgc cctcggcgat
     4741 caccgcgagg tcgaagcgct cggccacgcc gctctcgggc gtgacgacgg cgccgtcgcc
     4801 atcggtcttc acgcccgcga cggcggtgcc gaagcgcgcg tgcaggccgg cgtcgcgcga
     4861 gccgagcgcg ccgtccgtcg tggcgcaggc gtcgaggaag gccttctgca gcggcgcgag
     4921 cagcgtgccg tagctcagca cggcgcccag cagcggcacg cccagctcgt cggcgcggat
     4981 gtgcaccacc ggctcgccga acggcatggc cagcgtcggc ggcgcctggg acacgtgcac
     5041 ctggcggatc ggcgcggcct ggccggccgg ccaggcacgc aggcgctcga gcacctgcac
     5101 gctgccgagc gacagcgcga gggtgcgcgg gtcgcccgag atgtccttgg cggcgtcgcg
     5161 cgtgtcgaac acgctgacgg accaggccgg gcagcgctgg cgcatgagca gcgccagcga
     5221 caggcccacc ggcccgccgc cgatcaccgc caggcgcggc ggggtcgagg atcggctgtc
     5281 gtgcgttgtc accatgggag cgagggtcgg gcggggaatg ctattgtggc ccgatgcctg
     5341 cgatcgcgtt gatacgcagg cgacgtcatg gagatacgcc aatgcaatac cgtcgactgg
     5401 gccgcgctgg cctgcaggtg agcgagctgt cgctcggatc gtgggtcacc tatgccaacc
     5461 aggtggacgt cgacgccgcc gtcgagatga tggccgccgc ctacgacgcc ggcgtcaact
     5521 tcttcgacaa cgccgaggcc tacgccggcg gcaagagcga ggaggtgatg ggcgcggcgc
     5581 tcaagaagct cggctgggcg cgcctgaact acacggtgtc gaccaagttc ttctggggcc
     5641 tgccggacgc ggtcaccgtc aaccgcaaga acacgctgaa ccgcaagtac ctgatgcagg
     5701 ccatcgacgg ctcgctgcag cgcttcggcc tggaccacct cgacctcgtc tactgccacc
     5761 gcgccgaccc gaacacgccc atcgaggaga ccgtgcacgc gatgagcgac atgatcaccc
     5821 agggcaaggc gctgtactgg ggcacgagcg agtggtcggc gtccgacatc cgcgccgcct
     5881 gggagatcgc cgagcgccat cacctgcaca agccggtcgt ggagcagccg cagtaccacc
     5941 tgttccaccg ccagaaggtc gagcaggagt tcgcgcgcct gtacgacgac atcggcctgg
     6001 gcctgaccac ctggagcccg ctggcctcgg gcctgctcac cggcaagtac aagtccggca
     6061 tccccggcgg cagccgcggc gcgatgcaga acatgagctt cctgcacgac cagctcaccg
     6121 accaggccaa gaacgacgcc gtcgccaggc tggagccgat cgcgcgcgac ctgggcgcca
     6181 cgaccgcgca gctggccatc gcctgggtcg cgaagaaccc gcgcgtgagc acggtcatca
     6241 cgggcgcttc cagcgtcaag cagctcaagg acaacctgaa ggcctccgag atcgtcgcga
     6301 agctgacgcc ggacgtgctg gcgcgcatcg acgagatcac gaagccgctg gcggcctgag
     6361 cgccgcgcgc cgcatgcgaa ggcccggttc gccgggccga cgcgtcaccg agcggggtca
     6421 ggtcttgaat gttgcgcggg ccggcgtcat ccggatgggg tcaggtcttg aatcttgcac
     6481 ggcagcgcct tgatcgcaga cgacaggttg agcgtgcaag attcaagacc tgaccccatc
     6541 tttcggaggc ccggttcgcc gggcctttca ttgtggcggg cagggaccag aggggtctgg
     6601 cctctcctca tgttgccgcc gcggcgcagc cgatggcggt ggcgccgccg gcgacatgag
     6661 gcgaaggcct gacctcgaac atcagccgac gatcgaggtc aggccttcgc ctcgacatcg
     6721 cgatcggccg tgcagccttc ggcggcggca tggcgcgacg tcaaggagag gccagaccgc
     6781 tgcagccgtg gcgcgccgtc gtcagtcccc ggcgttcaac gccgccagcc gctccatcgt
     6841 cccgacatcg gtccaccgcc cgtcatactt cttcgcccgc agccgccgct ggtcgagcgc
     6901 gaattccagt ttcggccgca gcgcgagctt cgtgcccacc ggaacgtcgg cgaacagtgc
     6961 ggcgcgaaag agcccgatac tggcccaggt gaacttcggc gtggcgtcgc ggctcgcgag
     7021 gccgtcggcg gtgatgccga agtcgccgcg cggatggtgc ggcgcgttgt cgaccatcca
     7081 caactggccc agcgcgccgg agtcggcgaa ggcctgccca tcggcggggt cgaagcggaa
     7141 atcgggcacg aagacgtcgc cggacacgac ccagaacgcc tcgcccagca gcggcagcgc
     7201 cttggcgatg ccgccggcgg tctccagcgc gccgccgtgg tcgcggccct ccagcgaata
     7261 ggtgatcgcc agcccgtagc gtgcgccgtc gcccaaggcc gcggggaact gctcctccag
     7321 ccacgcc
//
LOCUS       DATCRO010000859         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_437253, whole genome shotgun sequence.
ACCESSION   DATCRO010000859 DATCRO010000000
VERSION     DATCRO010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437253"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1063
                     /gene="xylF"
                     /locus_tag="VIN75_23415"
     CDS             41..1063
                     /gene="xylF"
                     /locus_tag="VIN75_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8977182.1"
                     /translation="MNLKHALLTAGLLAGAAAAHAQVVVGVSWSNFQEERWKTDEAAI
                     KAELAKRGATYVSADAGGSPEKQLADVDGLIAKGAKALVILATDKDAIVPALAKAKQR
                     GIPVIAYDRLIDQPGVYYITFDNQEVGRMQARAVFSVKPTGNYVMIKGSPTDPNADFL
                     RAGQQEVLAAAIKSGAIKIVGEEYTEGWKPEVAQKNMEQILTRNNNKVDAVVASNDGT
                     AGGVVAALSARGISGVPVSGQDGDQAALNRVALGTQTVSVWKDARDLGREAADAAVDL
                     ASGKPVPGTKKWTGGEHKVTLDSKFLKPVPITRANLDVVVKAGWISKDNLCRGVDAAK
                     APPACK"
     gene            1123..2301
                     /locus_tag="VIN75_23420"
     CDS             1123..2301
                     /locus_tag="VIN75_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8977183.1"
                     /translation="MESLQLSALDLRLGLMAVVLAVMAATFHVLTGNFLTPENLYNIA
                     QQTAVVGIVATAISLVIVARHIDLSVGSVVGFVGVLVAYLQYTAGWSWTWACLAGLAA
                     ALLVGAYQGGLTAYLGVPSFVVTLGGLMSFRGAAFIVANGKTQPVTDPVFLWFGGGLD
                     GALGIAGSWTLAAVLALLLCWRVVAARRAKTRHGFPLRPLAADVAFTAGLLALLAAFT
                     AAMCAYQLPGKPQGQGVPAPVVIWGAVVIVVAFIVRRTRFGRYVYAIGGNPEAARLVG
                     IPVRRVTLQLFLLLSVTITIAAIVAVSRLNAGTNSMGTGMELYVIAAAVIGGVSLSGG
                     SGTVLGSVLGALIIQFLDSGLLLMDVGIGQRMIIIGQVLIVAVAFDVLYRRWTGERLA
                     "
BASE COUNT          342 a          831 c          830 g          339 t
ORIGIN      
        1 aaagggcgag acgacgcacg acaacaaccg gagacaagcc atgaacctca agcacgcact
       61 cttgaccgcc ggcctcctcg cgggcgccgc cgcggcccac gcccaggtgg tggtcggcgt
      121 gagctggtcc aacttccagg aagaacgctg gaagacggac gaagccgcca tcaaggccga
      181 actggccaag cggggcgcca cctacgtcag cgccgacgcg ggcggctcgc ccgagaagca
      241 gctggccgac gtggacggcc tgatcgccaa gggcgcgaag gcgctggtga tcctggcgac
      301 ggacaaggac gccatcgtcc ctgccctcgc caaggccaag cagcgcggca tccccgtcat
      361 cgcctacgac cgcctgatcg accagcccgg cgtctactac atcaccttcg acaaccagga
      421 ggtcggccgc atgcaggcgc gcgccgtgtt ctccgtcaag cccacgggca actacgtgat
      481 gatcaagggc tcgcccaccg acccgaacgc ggacttcctg cgcgcgggcc agcaggaggt
      541 gctcgccgcg gccatcaagt cgggcgcgat caagatcgtc ggcgaggagt acaccgaggg
      601 ctggaagccg gaggtggcgc agaagaacat ggagcagatc ctcacgcgca acaacaacaa
      661 ggtcgacgcg gtggtggcgt ccaacgacgg caccgccggc ggcgtggtgg cggcgctgtc
      721 ggcgcgcggc atctcgggcg tgccggtgtc gggccaggac ggcgaccagg cggcgctcaa
      781 ccgcgtggcg ctcggcacgc agacggtgtc ggtctggaag gacgcgcgcg acctcggccg
      841 cgaggccgcc gacgcggcgg tcgacctggc cagcggcaag ccggtgcccg gcacgaagaa
      901 atggacgggc ggcgagcaca aggtgacgct ggactccaag ttcctcaagc cggtgccgat
      961 cacccgcgcc aacctcgacg tcgtcgtcaa ggccggctgg atcagcaagg acaacctgtg
     1021 ccgcggcgtc gacgcggcca aggcgccccc ggcctgcaag tgaagccccg cgcggccgcg
     1081 cgtgcgtgac gcgcgcgcgg ccgcggcgca acatcccctc ccatggaatc gctccagctc
     1141 agcgcgctcg acctgcggct cggcctgatg gccgtcgtcc tcgcggtcat ggcggccacc
     1201 ttccacgtgc tcaccggcaa cttcctgacg ccggagaacc tgtacaacat cgcgcagcag
     1261 acggcggtcg tgggcatcgt cgccaccgcg atctctctgg tgatcgtcgc gcgccacatc
     1321 gacctgtcgg tcggctcggt ggtgggcttc gtcggcgtgc tcgtggccta tctccagtac
     1381 accgcgggct ggagctggac gtgggcctgc ctggccggcc tcgccgcggc gctgctggtc
     1441 ggcgcgtacc agggcgggct caccgcctat ctcggcgtgc cgtcgttcgt cgtcacgctg
     1501 ggcggcctga tgtcgttccg cggcgcggcc ttcatcgtgg ccaacggcaa gacgcagccc
     1561 gtgaccgatc ccgtcttcct gtggttcggc ggcggcctcg acggcgccct cggcatcgcg
     1621 ggcagctgga cgctcgccgc cgtgctcgcg ctgctgctgt gctggcgcgt ggtggctgcg
     1681 cggcgcgcca agacgcgcca cggcttcccg ctgcggccgc tcgcggccga tgtcgcgttc
     1741 accgccggcc tgctcgcgct gctggccgcg ttcaccgcgg cgatgtgcgc ctaccagctg
     1801 ccgggcaagc cccagggcca gggcgtgccg gcgccggtgg tcatctgggg cgcggtcgtg
     1861 atcgtcgtcg ccttcatcgt gcggcgcacg cgcttcgggc gctacgtcta cgcgatcggc
     1921 ggcaaccccg aggcggcgcg cctcgtgggc atcccggtgc gccgcgtgac gctgcagctg
     1981 ttcctgctgc tgtcggtgac gatcaccatc gccgcgatcg tcgccgtctc gcgcctgaac
     2041 gcgggcacca actcgatggg caccggcatg gagctgtacg tcatcgccgc ggccgtgatc
     2101 ggcggcgtga gcctgtcggg cggcagcggc accgtgctgg gctcggtgct gggcgcgctg
     2161 atcatccagt tcctggacag cggcctgctg ctgatggacg tgggcatcgg ccagcgcatg
     2221 atcatcatcg gccaggtgct gatcgtggcg gtcgccttcg acgtgctgta ccgtcgctgg
     2281 accggggagc ggctggcatg agcaacgcgc ctctggtcga gatcgtcggc atgcacaagg
     2341 cc
//
LOCUS       DATCRO010000860         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_212360, whole genome shotgun sequence.
ACCESSION   DATCRO010000860 DATCRO010000000
VERSION     DATCRO010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212360"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VIN75_23425"
     CDS             complement(<1..733)
                     /locus_tag="VIN75_23425"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8977184.1"
                     /translation="MQAPIPSGSTRRKLLVGGGAAALLASLYALAQRRRAPEQARAGI
                     RIAVPTVPHAGLVHIAAARGLFAGKGLDVTLLPQSYGKAAVAELVRGNADLAVAADVP
                     IVVEILKGAPLSIVASVANASNELAVLGRIDRGIRSAADLPGHKVGVTLGTSGEYFLW
                     AFLVRHRIAPQSVELVDLPPSGLIDALRRGIVDAITAWQPVRHEAELAFGDRAVSLSA
                     PDAYAQTYALVGRQDYVQAHRRPLGR"
     gene            complement(1488..1694)
                     /locus_tag="VIN75_23430"
     CDS             complement(1488..1694)
                     /locus_tag="VIN75_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977185.1"
                     /translation="MNTKPSPPGKDNAPSPGDRRGRPVQTGALPGGQPGVGVNTSPYE
                     TGQSNVDAAVQQGTEYGKDNLRQR"
     gene            complement(1790..3169)
                     /locus_tag="VIN75_23435"
     CDS             complement(1790..3169)
                     /locus_tag="VIN75_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY8977186.1"
                     /translation="MTHDASRRAFLKRSSMLAMAGTAAPWALSLAAMSEAAASTATDY
                     KALVCVFLYGGNDYGNTLIPYDAARYAAYRDMRPTQAYTQAQLAGTVLSPGVALAGGM
                     QYALAPELLPLKAIFDSGKMGVMLNVGTLVQPTTKAQYTAGNVPLPPRLFSHNDQQSV
                     WQASSPEGATSGWGGRMGDLFESGNGNAVFTCINVSSNAVYLSGRTATQYQVSTNGPV
                     ALNGVAKPLFGSSACSTALQNLVTASSANLFENEYGRISARSLGAGGALSAALAAGPA
                     ISTPFPTPGTNPLADQLKLVARMISTADAVGAKRQVFFVSMGGFDNHDGLATIHPGLL
                     TSVAQALGAFHAATQELKVDGQVTTFTASDFGRTLTANDGSDHGWGSMHFILGGAVNG
                     GRYYGTPPVVANDGPDDVGQGRLLPSTSVDQYAATMGKWLGISDTDLLTVLPNLANWD
                     ASQRNLGFV"
     gene            complement(3177..>4142)
                     /locus_tag="VIN75_23440"
     CDS             complement(3177..>4142)
                     /locus_tag="VIN75_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY8977187.1"
                     /translation="QQVPTYGLPDITGLARVFTGWDFDLQGQNVGTGTATPDFLKRPM
                     IQVPARYETGAKTFLGTTIPAGTDATTGLAMALDAISTHRNVAPFLSSQLIQRLVTSN
                     PSPAYVARIAAVFDDDGTGTRGNLKAVVRALLLDAEARTPGDATAYGKLREPILRFTG
                     WARAYGVTSPSGLWDVGDTSDAAKGLAQSPGRSPSVFNFFRPGYVPPDTALDANELEA
                     PEFQIANESSVVGCINYLQRAVQGSIADLTPDYTSLLALADDAGALLAEINLVLAADQ
                     VSAATIAMLAAAVSTMPTGTDTTRRARVNAALVLVLAAPEYIVQK"
BASE COUNT          567 a         1403 c         1546 g          626 t
ORIGIN      
        1 cccgccccag gggccgccgg tgggcctgca cgtagtcctg gcggcccacc agggcgtagg
       61 tctgcgcata ggcgtcgggc gcgctcaggg agacggcgcg atcgccgaac gccagctcgg
      121 cctcgtgccg cacgggctgc caggcggtga tggcgtccac gatgccgcgt ctgagggcgt
      181 cgatgaggcc cgagggcggc aggtcgacga gctcgaccga ctgcggcgcg atgcgatgcc
      241 gcaccaggaa ggcccacagg aaatattcgc cgctcgtccc gagcgtgacg cccaccttgt
      301 gcccgggcag gtcggccgcc gagcggatgc cgcggtcgat gcggcccagc accgccagct
      361 cgttggacgc gttggccacc gatgcaacga tgctcagcgg cgcgcccttg aggatctcga
      421 cgacgatcgg gacatccgcg gcgaccgcca ggtccgcgtt cccgcgcacc agctccgcca
      481 ccgcggcctt gccgtagctc tggggaagca gggtgacgtc caggcccttg ccggcgaaca
      541 gtccgcgcgc cgcggcgatg tgcaccaggc cggcatgcgg cacggtgggc acggcgatcc
      601 ggatgccggc ccgggcctgc tcgggcgctc gccttcgctg cgcgagcgca tacagcgacg
      661 ccagcaaggc ggcagcgccg ccgccgacca ggagcttgcg acgcgtcgac ccggacggga
      721 tcggagcctg cacttgcatc tcccccgacg acagcctcag ttcagcgcgt gcgggaaatg
      781 cctcgacgcg tccggggcga cggcggaagg ggccgcctgt cgcggccgcc gatcgatcgt
      841 acggcgcggc gcggcgactt gtccgacggt accgccgccg gccaggccgc gggtcgggct
      901 cggtgacgaa gacgaaagct tcccttgcat gcgggcctcc cagcctgtag cgcgagcaca
      961 ccggcaccgc acgccgatcg tggcgcgtcg ttccgtgaca ggaccgttga cgtcctgcag
     1021 accctcgcta ctgcatgcat cgacggcccg gcggagccag acgtcgttgt tggatttgtc
     1081 gagcagtact tccctggcga ctcgtcgcca tgcgtcgagt cgtgaatgga tagtagacgg
     1141 ttttgttcga tccgtcgtgc atttcgtggg ccggcaggac gattcgacgc gggccggcgt
     1201 gtcggcgccc caacgcatgc ggtcgaaccg gcaccgacgt gccggccggc gcgtcctcgc
     1261 gcgcgcgaaa acgcgacaag cggcacgatc ccgacatcgg cgtgccggcg cgttgcatga
     1321 cgacgcctac cagctcgcgg cgagcgagtt cgcatcgaag cgcgcatccc ccgtgatcgc
     1381 gctcaggtcg acgaactcga agtcgtcggc atgcagcgtc tggatctgcg ggatggtgct
     1441 gccgctgcat gccggatcgg gggcgccggc aagcgcgacg ccggcgctca gcgctggcgc
     1501 aggttgtcct tgccgtattc cgtgccctgc tgcacggccg cgtcgacgtt cgactgtccc
     1561 gtctcgtagg gcgacgtgtt gacgcccacg ccgggctgcc cgcccggcag cgcgccggtc
     1621 tgcacgggcc ggccgcggcg atcgcccggc gatggcgcgt tgtccttgcc gggcggcgaa
     1681 ggtttcgtgt tcatggcggc ctccgttgac tgcgatggcc ttcggcggca acggccgttc
     1741 ccgagcgctt tcagggtgcc gcgtcaagct gacgctggtc gcagcctgct caaacaaacc
     1801 ccaggttgcg ctgcgatgca tcccagttcg cgaggttcgg cagcaccgtc agcaggtcgg
     1861 tgtcgctgat gcccagccac ttgcccatcg tcgcggcgta ctggtcgacc gacgtggaag
     1921 gcagcaggcg gccctggccg acgtcgtccg ggccgtcgtt ggccaccacc ggcggcgtgc
     1981 cgtagtagcg cccgccgttg acggcgccgc ccaggatgaa gtgcatgctg ccccagccgt
     2041 ggtcggagcc gtcgttggcg gtgagcgtgc ggccgaagtc cgaggccgtg aacgtcgtga
     2101 cctgaccgtc caccttgagc tcctgcgtcg cggcgtggaa cgcgccgagc gcctgtgcca
     2161 cgctggtgag caggccgggg tggatcgtgg cgaggccgtc gtggttgtcg aagccgccca
     2221 tcgacacgaa gaacacctgg cgcttcgcgc ccacggcgtc ggcggtactg atcatgcgcg
     2281 ccaccagctt gagctggtcg gccagcgggt tcgtgccggg cgtgggaaag ggcgtgctga
     2341 tcgccggccc cgcggccagc gccgccgaca gcgcgccccc ggcgcccagc gagcgcgcgc
     2401 tgatgcggcc gtactcgttc tcgaacaggt tcgcgctgga ggccgtcacg aggttctgca
     2461 gcgcggtcga gcacgcgctc gatccgaaca gcggcttggc cacgccgttc agcgcgaccg
     2521 gcccgttggt ggacacctgg tactgcgtgg ccgtcctgcc cgagaggtac accgcattgc
     2581 tcgacacgtt gatgcaggtg aagacggcat tgccgttgcc cgactcgaac aggtcgccca
     2641 tgcggccgcc ccagccggac gtggcgccct cgggcgacga ggcctgccac accgactgct
     2701 ggtcgttgtg cgagaacagg cgcgggggaa gcggcacgtt gcccgcggtg tactgcgcct
     2761 tggtggtggg ctgcaccagc gtgccgacgt tgagcatcac gcccatcttc ccgctgtcga
     2821 agatggcctt gagcggcagc agctcgggtg cgagcgcgta ctgcatgccg ccggccaggg
     2881 cgacgccggg gctcagcacc gtgccggcca gctgggcctg cgtgtaggcc tgggtgggac
     2941 gcatgtcccg gtaggcggcg tagcgcgcgg cgtcatacgg gatgagggta ttgccgtagt
     3001 cgttgccgcc gtacaggaac acgcacacca gcgccttgta gtcggtggcg gtcgacgcgg
     3061 cggcctcgct catcgcggcc aggctcagcg cccagggcgc ggcagtgccg gccatcgcga
     3121 gcatcgagga gcgcttgagg aacgcgcggc gggaagcgtc gtgggtcatg cgggcctcac
     3181 ttctggacga tgtattcggg cgcggccagc accagcacga gggccgcgtt gactcgggcg
     3241 cggcgcgtcg tgtcggtgcc ggtgggcatc gtggagacgg ccgccgcgag catggcgatc
     3301 gtcgcggcgc tcacctggtc ggcggccagc acgaggttga tctccgccag cagcgcgccg
     3361 gcgtcgtcgg ccagcgccag cagcgacgtg tagtcgggcg tgaggtcggc gatcgagccc
     3421 tgcaccgcgc gctgcaggta gttgatgcag cccaccaccg aggactcgtt ggcgatctgg
     3481 aactcgggcg cctcgagctc gttcgcgtcg agcgccgtgt cgggcgggac gtagccgggc
     3541 cggaagaagt tgaacaccga cggcgagcgg ccggggctct gcgccaggcc cttggcggcg
     3601 tcggaggtgt cgccgacgtc ccacaggccg ctcggcgacg tgacgccgta cgcgcgcgcc
     3661 cagccggtga agcgcaggat gggctcgcgc agcttgccgt aggcggtcgc gtcgccgggc
     3721 gtgcgggcct cggcgtccag cagcagcgcc cgcacgacgg ccttgaggtt gccgcgcgtg
     3781 ccggtgccgt cgtcgtcgaa caccgcggcg atgcgcgcga cgtaggcagg gctcgggttg
     3841 ctggtgacca ggcgctggat cagctggctg ctgaggaagg gagcgacatt ccggtgcgtg
     3901 gagatggcat cgagcgccat tgccaggccg gtcgtcgcat cggtcccggc ggggatggtg
     3961 gtgccgagga aggtcttcgc gccggtctcg tagcgcgcgg ggacctggat catcgggcgc
     4021 ttcaggaagt cgggcgtcgc ggtgccggtg cccacgttct ggccctgcag gtcgaagtcc
     4081 cagccggtga acacgcgtgc caggccggtg atgtcgggca ggccgtaggt aggcacctgc
     4141 tg
//
LOCUS       DATCRO010000861         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_762994, whole genome shotgun sequence.
ACCESSION   DATCRO010000861 DATCRO010000000
VERSION     DATCRO010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762994"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..2473
                     /locus_tag="VIN75_23445"
     CDS             188..2473
                     /locus_tag="VIN75_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8977188.1"
                     /translation="MEIYLDCNATTPVLPQARQAALAAMADDFGNPSSIHSTGLRARS
                     LIDDVRARARRVLGAPTGRLLFTSGATEGIQTAVLSALVALRDRRERGEAMPALLLYG
                     ATEHKAVPESLRHWNALLELGLDVQAIPVGADGRHDLAWLRERAPRAGLVCTMAANNE
                     TGVISDLDGLAEVLAGSPALWLVDGVQALGKLPLRLAERRIDYAPFSGHKLYAPKGIG
                     LLYVREGAPFTPLMAGGGQEGAQRSGTENMTGVAALGAVLAALEDGGTFRDGATLAGF
                     RDRLAHALLDAFPTLVFNAPPALCLPTTLNFSVPGLPSKLLLDLFDAADVRVSGGSAC
                     SASKAQPSYVLQAMGLPAWQTASAVRLSFGPAADEAFIAAACERIRACGRSLQDSCLL
                     PSAPKSLPPEGLTRFIVDGACCYLLADATSRRCVVVDPLPELTERLAALLRCQRYTLA
                     AVLDTHSHGDHASSADALRAALPELLDDAGPLDALGWPADACELRLGDRVLTRLPIPG
                     HTTDSTAYLLHDADGLRLAFVGDTVMPGALGRSDFDQSEPLAFGASLLKLAQVVGSTT
                     LLLPAHDYDDRFASTLATERRSQPLLARMLGGGLDGPAFAAAKAELERGLGRSEYATL
                     ACGARVDPHVDDACNELAPEQLLALVERQPELLLVDVREAYEQRLGHAPDLVAPRARH
                     QAIPLSQLLLALPDWLALPTTTPVVFYCRSGNRSKQAANALRRLGHEQAWSLAGGLAL
                     WRPQGGDVPAPLPYPDVPAFA"
     gene            complement(2679..3035)
                     /locus_tag="VIN75_23450"
     CDS             complement(2679..3035)
                     /locus_tag="VIN75_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977189.1"
                     /translation="MISGNVIGGGGGIFAFVGGGNALGGAAGAGASTTATNIGFASIA
                     TSRGPLASRSPTTAAACTATSAAMAKRRRPARADVIAWRCAGPAAAGTSDRVAVRCAG
                     SARGAIAPPAAWLDSR"
     gene            3016..>3332
                     /locus_tag="VIN75_23455"
     CDS             3016..>3332
                     /locus_tag="VIN75_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8977190.1"
                     /translation="MTLPLITPDVSPAPAPAPLVTQVPAPPPQAAPAAPVETPPAPAP
                     SLSRTIPPSGIQYIVPPAPVYSRISARMRESGKAVVRVFIGEDGLPRTVQLATSTGFA
                     RLD"
BASE COUNT          427 a         1298 c         1150 g          457 t
ORIGIN      
        1 ggggtgtcgc ggcggccagg ggcggcgtga cgccggcgcg ggaatggatt tccaaaccgg
       61 cgggcctgga gggccggttg tggtacagca ttccggaatg ggcgatccgc gcagcatgaa
      121 atctccacgt tacccgaata cgatgcttgt ccatgccaca tcggcgaccc caccggagcc
      181 cgacgccatg gagatctacc tcgactgcaa cgccaccacc cccgtgctgc cccaggcgcg
      241 acaggcggcg ctggccgcga tggcggacga cttcggcaat ccgagcagca tccacagcac
      301 agggctgcgc gcgcgctcgc tgatcgacga cgtccgcgcc cgcgcgcgac gcgtgctggg
      361 cgctcccacc ggccggctgc tgttcacgag cggcgccacg gaggggatcc agacggcggt
      421 gctgtccgcc ctggtggcgc tgcgcgaccg ccgcgagcgc ggcgaggcca tgccggcgct
      481 gctgctgtac ggcgcgaccg agcacaaggc ggtgcccgaa tcgctgcgcc actggaatgc
      541 gctgctcgaa ctcgggctgg acgtgcaggc gatcccggtc ggtgccgacg gccgccatga
      601 cctcgcgtgg ctgcgcgagc gcgcgccgcg cgcggggctg gtctgcacga tggccgccaa
      661 caacgagaca ggcgtcatca gcgacctcga cgggctcgca gaggtgctcg ccggcagccc
      721 ggccctctgg ctggtcgacg gcgtgcaggc gctcggcaag ctgccgttgc gcctggccga
      781 gcgccgcatc gactacgcgc cgttctccgg ccacaagctg tacgcgccca agggcatcgg
      841 cctgctgtac gtgcgcgaag gcgccccgtt cacgccgctg atggccggcg gcgggcagga
      901 aggcgcccag cgctcgggca ccgagaacat gaccggcgtc gccgccctcg gcgcggtgct
      961 ggccgcgctc gaggacggcg gaaccttccg cgacggcgcc accctcgccg gctttcgcga
     1021 tcgcctcgcg cacgcgctgc tggacgcgtt cccgacgctc gtgttcaacg ccccgcccgc
     1081 gctgtgcctg cccaccacgc tgaacttctc ggtgccgggc ctgccgtcca agctgctgct
     1141 ggacctgttc gacgcggccg acgtgcgcgt cagcggcggc tcggcgtgca gcgcgtccaa
     1201 ggcgcagccc agctacgtgc tgcaggcgat gggcctgccg gcctggcaga cggcgtcggc
     1261 cgtgcggctg tcgttcgggc ccgcggccga cgaggccttc atcgccgcgg cctgcgagcg
     1321 catccgcgcc tgcggccggt cgctgcaaga cagctgcctg ctcccgagcg cgcccaagtc
     1381 gctgccgcct gaaggactga cgcgcttcat cgtcgacggc gcgtgctgct acctgctggc
     1441 cgacgccacg agccgccgct gcgtcgtcgt cgatccgctg cccgagctga ccgagcgcct
     1501 ggccgcgctg ctgcgctgcc agcgctacac gctcgccgcg gtgctggaca cccacagcca
     1561 cggcgaccac gcatcgtcgg ccgacgcgct ccgcgccgca ctgccggagc tgctggacga
     1621 cgccggcccg ctggacgcgc tcggctggcc cgcggacgcc tgcgagttgc gcctgggcga
     1681 ccgcgtgctg acgcgcctgc ccatccctgg ccacaccacc gactccaccg cctacctgct
     1741 gcacgacgcc gacggactgc ggctcgcgtt cgtcggcgac acggtgatgc ccggcgccct
     1801 cggacgcagc gacttcgacc agagcgagcc gctcgccttc ggcgcctcgc tgctcaagct
     1861 cgcgcaggtg gtgggttcga cgaccctgct gctgccggcc cacgactacg acgaccgctt
     1921 cgccagcacg ctggccaccg agcgccgcag ccagccgctg ctggcgcgca tgctgggcgg
     1981 cggactggac gggcccgcgt tcgcggcggc caaggccgaa ctcgagcgcg gcctgggtcg
     2041 gagcgagtac gcgacgctgg cctgcggtgc gcgcgtcgat ccgcacgtcg acgacgcctg
     2101 caacgagctg gcgccggagc agctgctggc gctcgtcgaa cggcagccgg agctgctgct
     2161 ggtcgacgtg cgcgaggcct acgagcagcg cctgggccat gcgcccgacc tcgtcgcgcc
     2221 gcgcgcgcgc caccaggcga tcccgttgtc gcagctgctg ctggcgctgc ccgactggct
     2281 cgcgctgccc acgacgacgc cggtcgtctt ctattgccgc agcggcaacc gcagcaagca
     2341 ggcggccaac gcgctgcgcc gcctcggcca cgaacaggcc tggagcctgg cgggcgggct
     2401 ggccttgtgg cggccgcagg gcggcgacgt gccggcgccg ctgccctatc cggacgtgcc
     2461 ggccttcgcc tgacgcgatc ctctcccttc gacgacgccg gccctgtgcc ggcgtcgtcg
     2521 tttccgcgtc cgccagtgtc tgcgccacgt cgctgccccg gtcgccccgc gctgcacccc
     2581 cgtcgatgac ctttcaatca atgggcatgt taatgcgaat cgttcgcatt ccgaatatta
     2641 tctgaacgcc atcacagcca cggatcgccg atgagacctc aacgactgtc aagccaagcc
     2701 gccggtggtg cgatcgcacc gcgagccgag ccggcacagc gcacggccac gcggtcgctc
     2761 gtccccgccg cggccggccc ggcgcaacgc cacgcgatca cgtcggcgcg ggccggccga
     2821 cgacgcttgg ccatcgccgc gctggtcgcg gtgcatgccg ccgcggtcgt cggactcctg
     2881 gacgccagcg ggccgcgcga ggtcgcgatc gatgcgaagc cgatgttcgt cgccgtcgtc
     2941 gatgcgcctg cccctgccgc gccgcccagg gcattgccgc cgccgacgaa cgcgaagatc
     3001 ccgccaccgc cgccaatgac gttgccgctg atcacgcccg acgtctctcc cgcgcccgcg
     3061 ccggcgccgc tcgttacgca ggtgccggcg ccgcctccgc aggccgcgcc cgccgcaccg
     3121 gtcgagacgc cgccggcacc tgcgccgtcg ctgtcgcgaa cgatcccgcc gtccggcatc
     3181 cagtacatcg tgccgccggc gccggtctac tcgcgcatct ccgcccgcat gcgcgagtcc
     3241 ggcaaggccg tcgtgcgcgt gttcatcggt gaagacggcc tgccgcgcac cgtgcaactg
     3301 gccacctcca cgggctttgc gcgcctggac ga
//
LOCUS       DATCRO010000862         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_21707, whole genome shotgun sequence.
ACCESSION   DATCRO010000862 DATCRO010000000
VERSION     DATCRO010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21707"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VIN75_23460"
     CDS             complement(<1..421)
                     /locus_tag="VIN75_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8977191.1"
                     /translation="MNETNDRQGRGTVLVTGASRGIGRAIALAAARQGHDVVVHCRSG
                     LAQAGDVCAEIAALGAKARVLAFDVADRAAAAAALQADIAGHGAYYGVVCNAGIARDN
                     AFPAMSGEEWDAVVHTNLDGFYNVLHPVVMPMVQRRKP"
     gene            complement(427..921)
                     /locus_tag="VIN75_23465"
     CDS             complement(427..921)
                     /locus_tag="VIN75_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEY8977192.1"
                     /translation="MTGPNDIDAGPWPPHAMDAWVPHRGPLSLLDGVEHCDEHGIEAR
                     VRVPADGLFGGDDGVPAWVGIEYMAQAIAAWSGARARAAGGSPKIGYLLGSRRYEAAV
                     PVFPVGAELRVLAQCELMGDNGLGMFDCRIEQGGRVLASGRISVFEPPADSEQGSPSG
                     APRE"
     gene            complement(918..2099)
                     /locus_tag="VIN75_23470"
     CDS             complement(918..2099)
                     /locus_tag="VIN75_23470"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY8977193.1"
                     /translation="MTAIFLQAHACVCALGDTPEAIRAGLWADAPSGVAPTDAHTPGR
                     MLALGRVATPLPALDGLPHRERSRNNALLLAALAPLRERVQAAVDRFGPARVAVVLGV
                     STAGLDEGVAAIRQVHVGFGWPAHYDYALQEMGNAAQCAARELGVAGPAYAISTACSS
                     GAKALATGARLLRSGQVDAVVAGGVDALSGFTIAGFSALDAVSAERCNPLSANRRGIN
                     IGEGAALFLMGRDDGPVRLSGWGESGDAHHMSAPEPSGRGAVEAMQRALARGGVAAGE
                     VDYVNLHGTATEQNDAMESLAVERVLGRDVPVSSTKPLTGHALAAAGAIEAAFAWHAL
                     VDNPSGRLPVHWWDGAADPALPPLRPVAPGFALGRPVRHVLSNSFAFGGSNASLLFSQ
                     P"
     gene            complement(2096..2662)
                     /locus_tag="VIN75_23475"
     CDS             complement(2096..2662)
                     /locus_tag="VIN75_23475"
                     /inference="COORDINATES: protein motif:HMM:NF023089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HEY8977194.1"
                     /translation="MQRLIAIAATVLALAGCVTAPAPAAHATPQLRLAPALLGRPLNL
                     QQHLTLHAPGRDRQLDVLLEADARHVQMGLLAMGQVAARLDWDGTTLTESKASWWPQE
                     VSGSRILSDLQLTLWPVDAIQAALPAGWRLVEDGDVRTLTQDGQVVTVITRLTPLLVQ
                     LDQRREHFQLEIESRDLGNDAAASGNPT"
     gene            complement(2650..3882)
                     /locus_tag="VIN75_23480"
     CDS             complement(2650..3882)
                     /locus_tag="VIN75_23480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8977195.1"
                     /translation="MTLETTDVLIVGAGPAGSVAAAFLRQQGRRVLVLEREQFPRFSI
                     GESLLPQSMEYIEAAGMLRDVVEAGFQFKNGAAFAWGDKYTDFDFREKFSPGWGTTYQ
                     VQRGNFDKVLADAAARMGAEVRFRHEVTAVDLDGDRPRVSARDAEGNAYRVEARFVLD
                     ASGFGRTLPRLLKLESPSNFPVRGAIFTHVADHIPPDGTFDREKILITVHPRHPDVWF
                     WTIPFSRGRCSLGVVAAQDFLARYTGTEMERLRAIVAEDARLSTLLANAAWDTPARQI
                     VGYSANVKSLWGKGFALLGNAGEFLDPVFSSGVTIAFKSASLATQCLAREFAGETVDW
                     QADYAVPLKSGVDTFRTFVDAWYAGGFQKIIFHERHTPDIRRMISSILAGYAWDQNNP
                     YVAQAKRRLGVLEELCSA"
     gene            complement(3879..>5731)
                     /locus_tag="VIN75_23485"
     CDS             complement(3879..>5731)
                     /locus_tag="VIN75_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977196.1"
                     /translation="WPQWWLARASDTRARPRDGRLALTDGGLEWAVLLEETTGAAFSM
                     SGDATVSAALDKGRAAALAAVPGAKVLAGGVPLFAEHAASRARLEVNTIGWGSVAGVL
                     VLVFLAFRSLWPRLLMALSLLVGVAMALSVTSWVFGHVHLLTTVFGASLVGCAEDYGL
                     YYFASRQGAPDAPPLKLMSTLLPGLALALLTSVLGYVALGLPPFPGLRQMALFSVVGI
                     LGAFVTTVVWFPWIDRGAVRATRFSDWIVDGYARWPRLGATRRSWLVHALLLAACAAG
                     IARLNVDDDLRQLQGSPKSLLDAQREIGRLLGLAAPSQFFVVRGTSADDVLQREEALK
                     ARLDARVAHGELAGYRALSDWVPSQARQAADAALTLRVEDGVIAGVDQVLGETQKRLA
                     FGAAPLTLDRWLASRASTAARALWIGAVPRRDDGTAFASVVMLRGVRDLAALPGIATS
                     ADGLPGVRWVDQVQSVSALLGRYRHMMGLLLVAGHAAVLLALAWRYGRRAWRAWLPTA
                     VATAGTLALLALAGQPLQLFNVLALALLLGIGIDYGIFLLEREDSGAGSAWLSVVLGA
                     AGTWLSFGLLALSSTPALHAFGLTLLLGILIVALAAPLYRPLTNSLPKTP"
BASE COUNT          833 a         2128 c         2079 g          691 t
ORIGIN      
        1 ccggcttgcg gcgctggacc atgggcatca ccaccggatg cagcacgttg tagaagccgt
       61 cgaggttggt gtggacgacg gcgtcccact cctcgccgct cattgccggg aacgcgttgt
      121 cgcgcgcgat gcccgcgttg cagacgacgc cgtagtaggc gccatgcccg gcgatgtcgg
      181 cctgcagcgc cgccgcggcc gcggcgcggt cggcgacgtc gaacgccagc acgcgcgcct
      241 tcgcgccgag cgcggcgatc tcggcgcaga cgtcgcctgc ctgcgcgagg ccgctgcggc
      301 agtgcaccac cacgtcgtgg ccctggcgcg cggccgccag cgcgatcgcg cgcccgatgc
      361 cccggctcgc gccggtcacc agcacggtgc cgcgcccttg cctgtcgttg gtctcgttca
      421 ttctcgtcat tccctcggtg cgccggatgg cgacccttgc tctgaatcgg cgggcggctc
      481 gaacacgctg atgcgtccgc tcgcgagcac gcgcccgccc tgctcgatgc ggcagtcgaa
      541 catgcccagc ccgttgtcgc ccatcagctc gcactgcgcc agcacgcgca gttcggcgcc
      601 gacgggaaac accggcaccg ccgcctcgta gcggcggctg cccagcaggt agccgatttt
      661 cggcgatccg cccgcggcgc gggcgcgcgc gcccgaccag gccgcgatgg cctgcgccat
      721 gtactcgatg ccgacccagg ccggcacgcc gtcgtcgccg ccgaacaggc cgtcggcggg
      781 cacgcgcacg cgcgcctcga tgccgtgctc gtcgcagtgc tcgacgccgt cgagcaggct
      841 cagcggcccg cgatgcggca cccacgcgtc catcgcgtgc ggcggccacg ggccggcgtc
      901 gatgtcgttc gggccggtca tggctgcgag aacagcaacg acgcgttgct gccgccgaaa
      961 gcgaacgagt tgctcaggac gtggcgcacg gggcgcccca gcgcgaagcc cggcgcgacg
     1021 gggcgcagcg gcggcagcgc ggggtcggcg gcgccgtccc accaatgcac cggcaggcgg
     1081 ccggacgggt tgtcgaccag cgcgtgccac gcgaacgcgg cctcgatggc gccggcggcc
     1141 gccagcgcgt gaccggtcag cggcttggtc gaactcaccg gcacgtcgcg gcccagcacg
     1201 cgttcgacgg ccaggctctc catcgcgtcg ttctgctcgg tggcggtgcc gtgcaggttg
     1261 acgtagtcga cctcgcccgc cgccacgccg ccccgggcca gcgcgcgctg catggcctcg
     1321 acggcgccgc ggccactggg ttccggcgcg gacatgtggt gcgcgtcgcc gctctcgccc
     1381 cagccggaca ggcgcacggg cccgtcgtcg cgccccatca ggaacagcgc ggcgccctcg
     1441 ccgatgttga tgccgcggcg gttggccgac agcgggttgc agcgctcggc cgacaccgcg
     1501 tccagcgccg agaagcccgc gatcgtgaag cccgacagcg catcgacgcc gccggccacg
     1561 accgcgtcga cctgccccga gcgcagcagc cgcgcgccgg tggccagcgc cttggcgccc
     1621 gacgagcacg ccgtcgagat ggcgtatgcc ggcccggcca cgcccagctc gcgcgcggcg
     1681 cactgcgcgg cgttgcccat ctcctgcagc gcgtagtcgt agtgcgccgg ccagccgaag
     1741 cccacatgca cctggcggat ggcagcgacg ccttcgtcca ggcccgcggt gctcacgccc
     1801 agcacgacgg ccacgcgcgc cggcccgaag cggtcgacgg cggcctgcac gcgctcgcgc
     1861 agcggcgcga gcgcggccag cagcagcgcg ttgttgcggc tgcgctcgcg atgcggcagc
     1921 ccgtccagcg cgggcagcgg cgtggccacg cgacccaggg cgagcatgcg tcccggggta
     1981 tgcgcgtcgg tcggtgcgac gccggaaggc gcgtccgccc acagcccggc gcggatcgcc
     2041 tcgggcgtgt cgcccagcgc gcagacgcag gcgtgggcct ggagaaagat ggcggtcatg
     2101 tcggattgcc ggaggcggca gcgtcgttgc cgaggtcgcg cgactcgatc tcgagctgga
     2161 aatgctcgcg ccgctggtcg agctggacca gcagcggggt gaggcgcgtg atcaccgtca
     2221 cgacctggcc gtcctgcgtg agcgtgcgca cgtcgccgtc ctcgacgagg cgccagcccg
     2281 cgggcagcgc ggcctggatc gcgtcgaccg gccacagcgt gagctgcagg tcgctcagga
     2341 tgcgcgagcc cgagacctcc tgcggccacc acgaggcctt gctctcggtg agcgtcgtgc
     2401 cgtcccaatc gaggcgtgcg gccacctggc ccatcgcgag caggcccatc tgcacgtgac
     2461 gcgcgtcggc ctccagcagc acgtcgagct gccggtcgcg gcccggcgcg tgcagcgtca
     2521 ggtgctgctg caggttcagt ggacggccca gcagcgccgg cgcgagccgc agctgcggcg
     2581 tcgcgtgcgc ggccggcgcg ggcgcggtga cgcagcccgc gagcgcgaga accgtcgcgg
     2641 cgatggcgat caggcgctgc acagttcctc cagcacgccc aggcgccgct tggcctgcgc
     2701 gacgtacggg ttgttctggt cccaggcata gccggccagg atcgacgaga tcatgcggcg
     2761 gatatcgggc gtgtggcgct cgtggaagat gatcttctgg aagccgccgg cgtaccacgc
     2821 gtcgacgaag gtgcggaagg tgtcgacccc ggacttcagc ggcacggcgt agtcggcctg
     2881 ccagtccacc gtctcgccgg cgaactcgcg cgccaggcac tgcgtggcga gcgaggccga
     2941 cttgaaggcg atcgtcacgc cgctggagaa caccgggtcg aggaactcgc ccgcgttgcc
     3001 cagcaacgcg aagcccttgc cccacagcga cttcacattg gccgagtagc cgacgatctg
     3061 gcgcgcgggc gtgtcccacg ccgcattcgc gagcagcgtc gacaggcggg cgtcttcggc
     3121 gacgatcgcg cgcagccgtt ccatctcggt gcccgtgtag cgcgcgagga aatcctgggc
     3181 cgcgaccacg ccgagggaac agcgtccgcg cgagaacggg atcgtccaga accagacgtc
     3241 gggatgcctc gggtgcacgg tgatgaggat cttctcgcga tcgaacgtcc cgtccggcgg
     3301 aatgtggtcg gccacgtgcg tgaagatggc gccgcgcacc gggaagttcg acggcgactc
     3361 gagcttgagc aggcgcggca gcgtgcggcc gaagccgctc gcgtcgagca cgaagcgcgc
     3421 ctcgacccgg tacgcgttgc cttcggcgtc gcgggccgac acacgcggcc tgtcgccgtc
     3481 gaggtcgacc gccgtcacct cgtggcggaa acgcacttcg gcgcccatgc gtgcggcggc
     3541 gtcggccagc accttgtcga agttgccgcg ctgcacctgg taggtggtgc cccatccggg
     3601 cgagaacttc tcgcggaagt cgaagtcggt gtacttgtca ccccatgcga acgccgcgcc
     3661 gttcttgaac tggaagccgg cctcgacgac atcgcgcagc atccccgcgg cctcgatgta
     3721 ctccatgctc tgcggcaaca ggctctcgcc gatcgagaag cgcgggaact gctcgcgctc
     3781 gagcacgagc acgcggcgtc cctgctggcg caggaaagcg gcggccacgg agccggcggg
     3841 gccggcgccg acgatcagga catcggtggt ttcgagagtc atggcgtttt cgggagtgaa
     3901 ttcgtaaggg gacggtacag cggcgcggca agcgcgacga tgaggatgcc caggagaagc
     3961 gtcaatccaa aggcatgcag cgcgggcgtg ctggacagcg cgagcagtcc gaacgacagc
     4021 cacgtgccgg cggcgcccag cacgaccgac agccaggccg agccggcgcc gctgtcctcg
     4081 cgctcgagca ggaagatgcc gtagtcgatg ccgatgccca gcagcagcgc cagcgccagc
     4141 acgttgaaca gttgcagcgg ctgtcccgcc agcgccagca gcgcgagcgt gccggcggtg
     4201 gccaccgcgg tgggcagcca cgcgcgccac gcgcggcggc cgtagcgcca ggccagcgcc
     4261 agcaagaccg ccgcgtggcc ggcgaccagc agcaggccca tcatgtggcg gtagcgcccg
     4321 agcagcgccg acaccgactg cacctggtcg acccagcgca cgccgggcag gccgtcggcg
     4381 ctcgtcgcga tgccgggcag cgccgcgagg tcgcgcacgc cgcgcagcat cacgacgctc
     4441 gcgaacgccg tgccgtcatc gcgccggggc acggcgccga tccacagcgc gcgggccgcg
     4501 gtcgaggccc gcgacgcgag ccacctgtcg agcgtcagcg gcgcggcgcc gaacgcgagc
     4561 cgcttctgcg tctcgcccag cacctggtcg acgccggcga tcacgccgtc ctcgacgcgc
     4621 agggtcaacg ccgcgtccgc ggcctggcgc gcctgcgagg gcacccagtc cgacagcgcc
     4681 cggtagccgg ccagctcgcc gtgcgcgacg cgcgcgtcga gtcgcgcctt gagcgcctcc
     4741 tcgcgctgca gcacgtcgtc ggcgctcgtg ccgcgcacga cgaagaactg cgagggcgcc
     4801 gccagcccca gcaggcggcc gatctcgcgc tgcgcgtcca gcagcgactt gggcgagccc
     4861 tgcagctggc gcaggtcgtc gtcgacgttc agccgcgcga tgccggccgc gcaggccgcc
     4921 agcagcagcg catgcacgag ccacgaccgg cgcgtggcgc ccaggcgcgg ccagcgcgcg
     4981 tagccgtcga cgatccagtc ggagaagcgc gtggcgcgca ccgcgccgcg gtcgatccac
     5041 gggaaccaga cgacggtcgt gacgaaggcg cccaggatgc ccacgaccga gaacagggcc
     5101 atctggcgca ggcccgggaa cggcggcagg ccgagcgcga cgtagcccag cacgctggtc
     5161 agcagcgcca gcgccaggcc cggcagcagc gtcgacatga gcttgagcgg cggcgcgtcc
     5221 ggcgcgccct ggcggctggc gaagtagtac aggccgtagt cctcggcgca gccgaccagg
     5281 cttgcgccga acacggtcgt cagcaggtgc acgtggccga acacccacga cgtcaccgac
     5341 agcgccatcg ccacgcccac cagcagcgac agcgccatca acaggcgcgg ccacagcgag
     5401 cggaacgcga ggaacaccag caccagcacg ccggccaccg agccccagcc gatcgtgttg
     5461 acctcgagcc tggcgcgcga ggcggcgtgt tccgcgaaca gcggcacgcc gccggccagc
     5521 accttcgcgc cggggacggc cgcgagcgcc gcggcccggc ccttgtcgag cgcggcagag
     5581 accgtcgcgt cgccgctcat cgagaacgcg gcgccggtcg tctcctccag cagcacggcc
     5641 cattcgaggc cgccgtcggt cagcgccagt cgaccatcgc gcggtcgggc gcgcgtgtcg
     5701 ctcgcgcggg cgagccacca ctgcggccac a
//
LOCUS       DATCRO010000863         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1111840, whole genome shotgun sequence.
ACCESSION   DATCRO010000863 DATCRO010000000
VERSION     DATCRO010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111840"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2464
                     /locus_tag="VIN75_23490"
     CDS             <1..2464
                     /locus_tag="VIN75_23490"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY8977197.1"
                     /translation="ASAARATDPTAGRGNWPTRGAAKLDVQVTGGRLAIADGPRGHRG
                     AAPLRIVTQGELSDWIGPQFRLARLQWSGRGSSDPQAPLEASVTLVEGQVGVARVTHA
                     QATLSGTLAQHQLQVDGEAPVSPPPWVAHLANIGNANATRLDAVLQGRWQAQATNTPA
                     WLGHVAQFDLRAANDPKAARPESAASAAAAASAAAAPTVANPNWVHVKPFDLSLSRDA
                     AGDLQTLRVAAGGAEFAGLALNWQPSFWTAPASPGASAHWAVDAALAPFSVAQLMARA
                     QPELGWHGDLQMTTTLHAAFDGQWHVAAQLARTSGDLSVTELVQDPNAAKQALGLTRA
                     TASVKAEGARWSADADIAGTRLGELTGHWTAEAPSPTSLPTPQSPLSGKVKAHVEDLN
                     VWGAWLPPGWRLGGEMRTELALSGRWGDPKLTGEVTATKLAIRNALEGVYGRDGEAAI
                     RLTGDQAQIERFRVQGGNGELTVTGNASLGAAPVARLQLQARQFQLLGRIDRKVVATG
                     DMSLQLGDDAIKVNGRLGIDEGLVDLSGGGAPTLDNDVEVLDGSTPAESDNPAAAVPK
                     ASRTTQINLAVDLGDKLRLRGRGIDTTLKGALQITSPGGLLTVRGTVRTAGGQYVAYG
                     QKLDISRGEIAFTGPVADPRLDILAVRPNLDVVVGVAITGTALAPHVRLFSEPEMSDS
                     DKLSWLMLGRAPETVGGADAALLQQAAMALLAGEGEAPSDQVLKRLGLTNFGVGQQTD
                     ANNVKQTVVTLGRQVSKRVYVGYERSVTNAAYNWQLIYRIAQRLTLRAQAGVEAVTTS
                     TTSTGNQAPSWSLDAIYTWRWN"
     gene            2645..3298
                     /locus_tag="VIN75_23495"
     CDS             2645..3298
                     /locus_tag="VIN75_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8977198.1"
                     /translation="MNTAPTPQPSSRPAAAAGGLHDDAEDRRLVACVCAHELAAFESL
                     YRRYHPRLTRFLHRMLRPEHLIEEVLNDTLHVVWNQAERFNGTSKVSTWIFAIAYRKA
                     LKALSRHEDPLPDTTEDDACHPAADPSERPEARMSLGQARAAIDAALRALSADQRAAV
                     ELTYFHGFSYPEIAQIVDCPTDTVKTRMFHARRRLRAWNMRVLTVSVGQSTICAISG"
BASE COUNT          485 a         1231 c         1200 g          382 t
ORIGIN      
        1 ggcatcggcc gcgcgtgcga cggacccgac cgccggccgc ggcaactggc ccacgcgcgg
       61 cgccgcgaag ctggacgtgc aggtgaccgg cggccggctc gcgatcgccg acggcccgcg
      121 cgggcatcgc ggcgcggcgc cgctgcgcat cgtcacgcag ggcgagctca gcgactggat
      181 cgggccgcag ttcaggctcg ccaggctgca gtggagcggc cgcggcagca gcgacccgca
      241 ggcgccgctg gaagcgtcgg tgacgctggt cgagggccag gtgggcgtcg cgcgcgtcac
      301 gcacgcgcag gcgacgctca gcggcacgct ggcgcagcac cagttgcagg tcgacggcga
      361 ggcgccggtc agcccgccgc cctgggtcgc gcacctggcc aacatcggca acgcgaacgc
      421 cacgcgcctg gacgccgtgc tgcagggccg ctggcaagcg caggcgacga acacgccggc
      481 ctggctgggg cacgtcgcgc agttcgacct gcgcgccgcc aacgatccca aggccgcccg
      541 gcccgagagc gcggcctcgg cggcggccgc ggcgtcggcg gccgcggcgc cgacggtggc
      601 caatccgaac tgggtgcacg tcaagccgtt cgacctgagc ctgtcgcgcg atgccgcggg
      661 cgacctgcag acgctgcgcg tggccgccgg cggcgcggag ttcgcgggcc tcgcgctcaa
      721 ctggcagccc tcgttctgga cggcgcccgc ttcgcccggc gccagcgcgc actgggcggt
      781 cgatgcggcg ctggcgccgt tctcggtggc gcagctgatg gcgcgcgcgc agcccgagct
      841 cggctggcac ggcgacctgc agatgaccac cacgctgcac gccgccttcg acggccagtg
      901 gcacgtggcg gcgcagctcg cgcgcaccag cggcgacctc agcgtgaccg agctcgtgca
      961 ggatccgaac gcggccaagc aggcgctcgg actcacgcgc gccacggcca gcgtcaaggc
     1021 cgagggcgcg cgctggagtg ccgacgccga catcgcgggc acgcggctgg gcgagctcac
     1081 gggccactgg acggccgagg cgccctctcc cacgtcgctg ccgacgccac agtcgccgct
     1141 gtcgggcaag gtcaaggcgc atgtcgagga cctgaacgtc tggggggcct ggctgccccc
     1201 gggctggcgc ctgggcggcg agatgcgcac cgagctcgcg ctgtcgggcc gctggggcga
     1261 ccccaagctc acgggcgagg tgacggccac gaagctggcg atccgcaatg cgctcgaggg
     1321 cgtctacggc cgcgacggcg aggccgcgat ccgcctgacc ggcgaccagg cgcagatcga
     1381 gcgcttccgc gtccagggcg gcaacggcga gctcaccgtc acgggcaacg cgagcctggg
     1441 cgcggccccg gtggcccggc tgcagctgca ggcgcggcag ttccagctgc tgggccgcat
     1501 cgaccgcaag gtcgtcgcca ccggcgacat gtccctgcag ctgggagacg acgcgatcaa
     1561 ggtcaacggc aggctgggca tcgacgaagg tctcgtcgac ctgagcggcg gcggcgcccc
     1621 gacgctggac aacgacgtcg aggtgctgga cggcagcacg ccggccgaga gcgacaaccc
     1681 ggccgccgcg gtgcccaagg ccagtcgcac gacgcagatc aacctggcgg tcgacctcgg
     1741 cgacaagctg cggctgcgcg gccgcggcat cgacaccacg ctcaagggcg cgctgcagat
     1801 cacctccccc ggcggcctgc tgaccgtgcg cggcaccgtg cgcacggccg gcggccagta
     1861 cgtcgcctac ggccagaagc tggacatctc gcgcggcgag atcgcgttca ccggccccgt
     1921 ggccgacccg cgcctggaca tcctggcggt gcggcccaac ctggacgtcg tcgtcggcgt
     1981 ggcgatcacc ggcaccgcgc tggccccgca cgtgcggctg ttcagcgagc ccgagatgag
     2041 cgacagcgac aagctgtcct ggctgatgct gggccgcgcg ccggagacgg tcggcggcgc
     2101 cgacgccgcg ctgctgcagc aggcggcgat ggccctgctg gccggcgagg gcgaggcgcc
     2161 cagcgaccag gtgctcaagc gcctgggcct gaccaacttc ggcgtcggcc agcagaccga
     2221 cgccaacaac gtcaagcaga ccgtcgtgac gctcgggcgc caggtctcca agcgcgtgta
     2281 cgtcggctac gagcgcagcg tcaccaacgc ggcctacaac tggcagctga tctaccggat
     2341 cgcccagcgc ctgaccctgc gcgcccaggc gggcgtcgag gcggtgacga cctcgacgac
     2401 gtcgacgggc aaccaggcgc cgtcgtggtc gctggacgcg atctacacgt ggcgctggaa
     2461 ttagggcctg ttgacaccag accgcctggc ggaccggagc ggccgctgat cgcccaggcg
     2521 ccccggcgcc gtccatttcg tgccacaagt cacgctcggg cggtgcgcat cggcggccgc
     2581 tgcgcgacga ttgaaccctt cccacgctcg acggcatgca cggtggacga agccctcgcc
     2641 agccatgaac accgccccca caccccagcc atcctcccgg cccgccgcgg ccgccggcgg
     2701 cctgcacgac gacgccgagg accgccgcct ggtggcctgc gtgtgcgcgc acgagctggc
     2761 cgccttcgaa tcgctgtacc gccgctacca tccgcggctg acgcgcttcc tgcaccgaat
     2821 gctgcggccg gagcacctga tcgaggaggt gctcaacgac acgctccacg tcgtctggaa
     2881 ccaggccgag cgcttcaacg gcacgagcaa ggtgtcgacc tggatcttcg cgatcgccta
     2941 ccgcaaggcg ctcaaggcgc tgtcgcgcca cgaggatccg ctgcccgaca cgaccgagga
     3001 cgacgcctgc cacccggcgg ccgacccgtc ggagcggccc gaggcgcgca tgagcctggg
     3061 gcaggcccgg gcggcgatcg acgccgcgct gcgggcgctg tcggccgacc agcgcgccgc
     3121 cgtcgagctg acctacttcc acggcttcag ctaccccgag atcgcgcaga tcgtcgactg
     3181 tcccaccgac accgtcaaga cgcgcatgtt ccacgcccgt aggcgcttgc gggcgtggaa
     3241 catgcgcgtc ttgacggtgt cggtgggaca gtcgacgatc tgcgcgatct cggggtag
//
LOCUS       DATCRO010000864         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_313468, whole genome shotgun sequence.
ACCESSION   DATCRO010000864 DATCRO010000000
VERSION     DATCRO010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313468"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VIN75_23500"
     CDS             <1..1410
                     /locus_tag="VIN75_23500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8977199.1"
                     /translation="DRLHAFGALCSHYHGPLAEGLVVGDTVRCPWHHASFDVTTGEAV
                     RAPALDPIACWRVEQRDGRAFVGERVIAGPRERPPVSGHPQSIVIVGGGVAGLAAAEM
                     LRRSGYTRPLTMVSADDAPPCDRPNLSKDFLAGTAEPDWIPLRPPGFYESNGIELLLS
                     TRVTAIDPARRVATLDSGRELAWGALLLATGADPIRMDIPGADPARVHVLRSFSDSKA
                     IVARCAGSKRALVIGSSFIGLEVAASLRARGLAVHVVGRDAVPMANVLGEELGRRIQA
                     LHEEHGVVFHLGTTVDRVDGAAACLADGSRVDGCDFIVAGIGVRPSLALAGQAMLAVD
                     RGVVVDASLRTSADGIWAAGDIARWPDARTGEPLRVEHFAHAQAQGQCAALNMLGARR
                     PFDAVPFFWSQHYDIAINMTGHASSWDRIEIDGSVQALDAAVRYFRGGRLLAMATVGR
                     DLENLRCEARMESEAAAPA"
     gene            complement(1415..3640)
                     /locus_tag="VIN75_23505"
     CDS             complement(1415..3640)
                     /locus_tag="VIN75_23505"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977200.1"
                     /translation="MPTILEVMSHITGMGLTAVARVTDHSWTACAVRDEIAFGLRAGD
                     ELDLETTICDEIRQHGQPVAFGSASADPRYREHVTPRLYGFESYVSIPIVRLDGTFFG
                     TLCGIAREPADPLAADTLHTLEFFAQLIAAQLEAQEASDRDACALAAATDATQRLERE
                     NRRIARLMREREAAEQAVTLELKDTRRLRDVAALVAIPGEGDRLFDEILDAAIDILDA
                     DAGTIQLLDPATQSLRFLATRGFGPDIVAHFANVDASSGSPCGAALSSGQHAFMVFDP
                     AAPDPDGSTRWHLDAGMRSAQSTPLVSRSGQPLGMFSTHWHVRRSLGERDLRFLDLLG
                     RQAADLIEHRQQQQALAASERRLREEARRKDEFIAVLAHELRNPLAPIRSGVELLRRT
                     SDPLIERVRPMMERQVRHMVRLIDDLLDVARISSGKVELRREPVTLERLVDGAVEPHR
                     GTVAASGIALDVELDDPDRVLDVDPTRLSQVLSNVIHNAAKFTPPGGRIAVAATLARP
                     GAGTPAQQVIRISDTGAGIRPELLPTIFELFTQLRPDSPEDSASRHGGMGIGLALARS
                     LVELHGGTIGADSAGPGRGTTFTIRIPVASSRSPPASDEPHDASDGRLDMRVLVVDDN
                     RDAADALGMLLSQHGAQVHVAYGAQDSVATTRRFQPALALLDLGMPDVDGYEACRRIR
                     GELGPDLRVFALTGWGQDEDRRNTVEAGFDAHLTKPVDIVRLIELATALVPGARVEHN
                     L"
BASE COUNT          465 a         1358 c         1416 g          519 t
ORIGIN      
        1 gaccgcctcc acgcgttcgg cgccctttgc agccactacc acgggccgct cgccgagggc
       61 ctggtcgtcg gcgacaccgt gcgctgcccg tggcaccacg ccagcttcga cgtcaccacg
      121 ggcgaggccg tgcgcgcgcc cgcgctcgac ccgatcgcgt gctggcgcgt cgagcagcgc
      181 gacggccgcg ccttcgtcgg cgagcgcgtc atcgccgggc cgcgcgagcg gccgccggtc
      241 tccgggcatc cgcagtccat cgtgatcgtc ggcggcggcg tcgcgggcct cgcggccgcc
      301 gagatgctgc ggcgctcggg ctacacgcgg ccgctgacga tggtgagcgc cgacgacgca
      361 ccgccctgcg accggcccaa cctgtccaag gatttcctgg ccggcaccgc cgagcccgac
      421 tggatcccgc tgcggccgcc cgggttctac gagtcgaacg gcatcgagct gctgctgtcc
      481 acgcgcgtga cggcgatcga tccggcgcgc cgcgtcgcga cgctggactc cggccgcgag
      541 ctcgcctggg gcgcactgct gctggccacc ggcgccgacc cgatccgcat ggacatcccc
      601 ggcgccgacc cggcgcgcgt gcacgtgctg cgcagctttt ccgacagcaa ggcgatcgtc
      661 gcccgctgcg ccggcagcaa gcgcgcgctg gtgatcggtt cgagcttcat cggcctggag
      721 gtggccgcgt cgctgcgtgc ccgcggcctc gcggtgcacg tggtcggacg cgacgccgtg
      781 cccatggcca acgtgctggg cgaggagctc gggcgccgca tccaggcgct ccacgaggag
      841 cacggcgtcg tgttccacct gggcaccacg gtcgatcgcg tcgacggcgc ggccgcctgc
      901 ctggccgatg gctcgcgcgt cgacggctgc gacttcatcg tggccggcat cggcgtgcgg
      961 ccgtcgctgg cgctggccgg gcaggcgatg ctggcggtcg accgcggggt ggtcgtcgac
     1021 gcctcgctgc gcaccagcgc cgacggcatc tgggccgcgg gcgacatcgc gcgctggccc
     1081 gacgcgcgca ccggcgagcc gctgcgggtc gagcacttcg cgcacgccca ggcgcagggc
     1141 cagtgcgcgg cgctgaacat gctcggcgca cgccggccgt tcgacgccgt gccgttcttc
     1201 tggagccagc actacgacat cgcgatcaac atgacgggcc atgcgtcgag ctgggaccgc
     1261 atcgagatcg acggctcggt gcaggcgctg gacgccgccg tgcgctattt ccgcggcggc
     1321 aggctgctgg cgatggccac cgtgggccgc gatctcgaga acctgcgctg cgaggcgcgc
     1381 atggaatcgg aggccgcggc gcccgcctga acgctcagag gttgtgctcg acgcgcgccc
     1441 ccggcacgag cgcggtggcc agctcgatga gccggacgat gtccaccggc ttggtcaggt
     1501 gggcatcgaa gccggcctcg accgtgttgc gccggtcctc gtcctggccc cagccggtga
     1561 gcgcgaacac ccgaagatcc gggcccagct cgccgcggat gcggcggcag gcctcgtagc
     1621 cgtcgacgtc gggcatgccc aggtccagca gcgccagcgc gggctggaac ctgcgggtcg
     1681 tcgccacgct gtcctgcgcg ccgtacgcga cgtgcacctg cgcgccgtgc tgcgacaaca
     1741 gcatgcccag cgcgtcggcc gcgtcgcggt tgtcgtcgac gacgagcacg cgcatgtcca
     1801 ggcggccgtc gctggcgtcg tgcggctcgt cggacgccgg cgggctgcgg ctcgaagcga
     1861 ccgggatgcg gatggtgaac gtggtgccgc gtcccgggcc ggcgctgtcg gcgccgatcg
     1921 tgccgccgtg cagctcgacc aggctgcgcg cgagcgccag gccgatgccc atgccgccgt
     1981 ggcgcgaggc gctgtcctcc gggctgtccg ggcgcagctg ggtgaacagc tcgaagatgg
     2041 tcggcagcag ctccggccgg ataccggctc ccgtatcgga gatgcggatc acctgctgcg
     2101 caggcgtccc ggcgccgggc cgcgccagcg tcgcagcgac cgcgatccgg ccgcccggcg
     2161 gcgtgaactt ggcggcgttg tggatcacgt tggacagcac ttgcgacagc cgcgtcgggt
     2221 cgacgtcgag gacgcggtcc gggtcgtcga gctcgacgtc cagcgcgatg ccgctcgcgg
     2281 ccaccgtgcc gcggtgcggc tcgacggcgc cgtcgacgag gcgctccagc gtcaccggct
     2341 cgcgccgcag ctcgaccttg cccgagctga tgcgtgcgac gtcgagcagg tcgtcgatga
     2401 gccgcaccat gtgcctgacc tggcgctcca tcatcggccg cacgcgctcg atcagcgggt
     2461 cggacgtgcg gcgcagcagc tcgacgccgc tgcggatggg cgcaagcggg ttgcgcagct
     2521 cgtgggccag caccgcgatg aactcgtcct tgcgccgcgc ctcctcgcgc aaccgccgct
     2581 cgctggcggc cagcgcctgc tgctgttgcc ggtgctcgat caggtcggcg gcctggcggc
     2641 ccagcaggtc gaggaagcgc aggtcgcgct cgcccaggga gcggcgcacg tgccaatgcg
     2701 tcgagaacat gcccagcggc tgccccgagc ggctgaccag cggcgtggac tgcgcgctgc
     2761 gcatgccggc gtccaggtgc cagcgggtcg agccgtcggg atcgggcgcg gcggggtcga
     2821 acaccatgaa ggcgtgctgg cccgacgaca gcgccgcgcc gcagggcgat cccgacgagg
     2881 cgtcgacgtt ggcgaaatgc gcgacgatgt cgggaccgaa gccgcgcgtg gcgaggaagc
     2941 gcaacgactg cgtcgccgga tcgagcagct ggatcgtgcc ggcgtcggcg tccaggatgt
     3001 cgatggccgc gtcgaggatc tcgtcgaaca ggcggtcgcc ctcgcccggg atggcgacga
     3061 gcgcggcgac gtcgcgcagc cggcgcgtgt ccttcagctc gagggtgacg gcctgttcgg
     3121 ccgcctcgcg ctcgcgcatc agccgcgcga tgcggcggtt ctcgcgctcg agccgctgcg
     3181 tggcatcggt ggccgcggcc agcgcgcacg cgtcgcggtc gctcgcctcc tgcgcctcga
     3241 gctgcgcggc gatcagctgg gcgaagaact cgagcgtgtg cagcgtgtcc gccgccagcg
     3301 ggtcggccgg ttcgcgcgcg atgccgcaca aggtgccgaa gaacgtgccg tccaggcgca
     3361 cgatggggat cgagacgtag ctctcgaagc cgtagagcct gggcgtgacg tgctcgcggt
     3421 agcgcggatc ggcgctggcg ctgccgaagg ccacgggctg gccgtgctgg cggatctcgt
     3481 cgcagatcgt cgtctccagg tccagctcgt ctccggcgcg caggccgaag gcgatctcgt
     3541 cgcgcaccgc gcaggcggtc cacgagtggt ccgtcacgcg cgccaccgcg gtcaggccca
     3601 tgccggtgat gtgcgacatc acctccagga tggtgggcac cccggggagc cggcttatcg
     3661 ccgcgacgtc gtcggcaact gttcgcatgg ctgaggagtc gaatgttccg ctgcgcaagg
     3721 gcggggcgcg caccgcggcg ggcctggccc gtcctgcg
//
LOCUS       DATCRO010000865        11687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1089389, whole genome shotgun sequence.
ACCESSION   DATCRO010000865 DATCRO010000000
VERSION     DATCRO010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089389"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..535
                     /locus_tag="VIN75_23510"
     CDS             203..535
                     /locus_tag="VIN75_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8977201.1"
                     /translation="MAIPHASSGQVVEILSSDGSLPSDQSYAVFKSDQLEVMRLVLPA
                     GKAFPPHRVAGEITVQCLSGSLELNVDGTRQVLRAGQLMFLRGGVEHGLVGIEEAIAL
                     VTIVLCKP"
     gene            639..1328
                     /gene="aqpZ"
                     /locus_tag="VIN75_23515"
     CDS             639..1328
                     /gene="aqpZ"
                     /locus_tag="VIN75_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY8977202.1"
                     /translation="MKQYGAEFIGTFWLVLGGCGSAVLAAAFPGVGIGLLGVSLAFGL
                     TVLTMAFAIGPISGCHLNPAVSVGLWAAGRFPANKLLPYIVAQVLGAIVAGGILYVIA
                     SGAPGFDVAKGFASNGYGVRSPGGYSMLAGLVCEVVMTAFFLIVILGSTDGRASAQFA
                     PIAIGLALTLIHLVSIPVTNTSVNPARSTGVAIYVGAAALEQLWLFWVAPIVGACLGA
                     QIFKAIRPTEI"
     gene            1457..1876
                     /locus_tag="VIN75_23520"
     CDS             1457..1876
                     /locus_tag="VIN75_23520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEY8977203.1"
                     /translation="MQIRPFRDSDEAAVIALWEAAGLTRSWNDPRKDIERKRAVQREG
                     FLVGTVGDVVMASIMIGYDGHRGWINYLAVDAAHRGQGHARTLMREAERLLEAAGCPK
                     INLQIRSSNASVIAFYQRIGYLQDDVVSFGRRLIADE"
     gene            complement(1968..3374)
                     /gene="dbpA"
                     /locus_tag="VIN75_23525"
     CDS             complement(1968..3374)
                     /gene="dbpA"
                     /locus_tag="VIN75_23525"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY8977204.1"
                     /translation="MNSPAPASSFDTLALPPNVLANLAQLGYVEMTPIQAAALPPALA
                     GQDIIAQAKTGSGKTAAFALAMLDKLNPRHFGVQALVLCPTRELAEQVAVETRRLARA
                     EENIKVLTVVGGVPVRNQLASLEHGAHVVIGTPGRVLDLLEREALDLSGLKTLVLDEA
                     DRMLDMGFVDDIKAVAGTCPKTRQTLLFSATYPEGIARLSQQFMRSPQMVKVESRHSA
                     AQIVQLWYEVEEQDRLHAVGRLLRHYQPANTIAFCNTKQQCRDLVDVLTAEGIQAIAL
                     HGDLEQRDRDQVLIQFANRSCSVLVATDVAARGLDVPQLACVVNVDVTYEPDQHIHRI
                     GRTGRAGEDGLALNLASLDEMGRVGNIEVLQKGETEWHELSELDARDDQPLVAPMVTL
                     QILGGRKEKMRPGDVLGALTKDMGVEGAKIGKINVTDMSTYVAVERAIADKVLAKLNA
                     GFVKGKRVRARRIEGNSE"
     gene            3458..4120
                     /locus_tag="VIN75_23530"
     CDS             3458..4120
                     /locus_tag="VIN75_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY8977205.1"
                     /translation="MTAADRQSSPAAERNRQPILQVLRRVLPPAGRALDIASGSGQHV
                     SWFARHLPGWTWQPSEYAAASLASIAAWSVLDEADLAESPQAVAGTGPLANIASPLQL
                     DVCSPEWPVSGVFDAITCINMLHASPPATLPGLMQGVGRHLARGGVLVTYGPYILDDE
                     PLAPSNVEFDRWLKTERDPSWGIRRLADVAAQAHDAGLRLRERVPMPANNLVLVFDRE
                     GA"
     gene            4122..4727
                     /locus_tag="VIN75_23535"
     CDS             4122..4727
                     /locus_tag="VIN75_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEY8977206.1"
                     /translation="MTNTQDASTDGVPAELAIAETRAWVRRAVIGLNLCPFARAVDAK
                     DQIRYVFSDATDPETLLATLVVELQRLADTDPQVVDTTMLIHPRVLADFEDFNDFLEL
                     ADAAVEDLDLDGVLQVASFHPRFQFADTEPDDITNATNRSPYPTLHLLREDSVDRAVA
                     AFPEAEAIFERNMETLDKLGAKGWADLRKSWEDDAAAELGS"
     gene            4784..6025
                     /locus_tag="VIN75_23540"
     CDS             4784..6025
                     /locus_tag="VIN75_23540"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEY8977207.1"
                     /translation="MAPPAPSPVVASLLVVDDNVVQRIQVVALCRELGIDMIYEAGSG
                     DEALELLSLLVLPPDVAILDLEMPGMDGIELIERLHERKLAIPLVVVSGREAVLIDTV
                     EAMARNLGMPVVAGIRKPLTREALAAALDGLERERTGPVTAAERAALPPIDPADLAAA
                     IASGGIAVHYQPKVDMARGAVCGVEALARWTHPRLGPVGPDRFIEVAEREGLIHALTL
                     AVLRQAFTDAARWRTGGLHLSLAVNLSPRLLDDPSLVRELSQLLDDHGLDARQVVLEI
                     TENSVVDCMGVALGVLARLRLKGFRLSIDDYGTGFSSMQQLARVPFTELKIDRAFVHG
                     AAQRTNLRVILKSALDMSRQLGLVTVAEGIETLPDWQLVRDSGCGIGQGFIVSRAMPA
                     DELPRWLEAHEARLRELGTGE"
     gene            complement(6035..6529)
                     /locus_tag="VIN75_23545"
     CDS             complement(6035..6529)
                     /locus_tag="VIN75_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977208.1"
                     /translation="MRRLLAALALTTFAGTAAAATAELLKPMAFLAGHCWKGTFPDGK
                     ATDEHCFFWMYGGSVLRDTHTVRRPGKPDMTGESTYFVDTVGNHVAFLYIESGGGSSR
                     GTVESLPEALLFPDTQYVSDGEALVYRARWTRQGDKAYEAWSEAQGPDGWTTMFKVLL
                     KRTD"
     gene            complement(6615..7115)
                     /locus_tag="VIN75_23550"
     CDS             complement(6615..7115)
                     /locus_tag="VIN75_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977209.1"
                     /translation="MPHPLSTLGIAHTAISLVPLVAGLCSLAIHRRIEPRSTAGRVYV
                     AGLVASVLTSFGLSSTGGFNPGHALGILALLALAGAWLVPRIEALGRTRDYLATLAMS
                     FSFFLLLVPGINETLTRLPPSQPIADGPMSAIVKGTLGVWFLLFLAGATLQVIGLRAR
                     RRADIA"
     gene            complement(7180..7806)
                     /locus_tag="VIN75_23555"
     CDS             complement(7180..7806)
                     /locus_tag="VIN75_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8977210.1"
                     /translation="MALDTSLLVGRLRGMDVELSPKASEIVACAQSLLAVRGYNGFSY
                     ADISEAVHISKPSVHHHFASKADLVRTVVRRYRDGARAGMAALEQQIADPLAQLHAYV
                     GYWTACIRDGTAPFCVCAMLAAEMPAIPGEVAEEVRGHFDDLAGWLQAVLERGVADGV
                     FHLRGPAKAEAMAVMASVHGAMLAARAHGDTELFGTIMKPVLHRLTHH"
     gene            complement(8033..8335)
                     /locus_tag="VIN75_23560"
     CDS             complement(8033..8335)
                     /locus_tag="VIN75_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEY8977211.1"
                     /translation="MRAVTTVIESTPLTLRELARAVGAEDQWIVRLVEVEILHVDTPA
                     DPPERWHFRSADLQRALAARRLERDFGANLDTAALILDLQSELRRVRALMHAHGLA"
     gene            complement(8337..9317)
                     /locus_tag="VIN75_23565"
     CDS             complement(8337..9317)
                     /locus_tag="VIN75_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY8977212.1"
                     /translation="MDFKDYYSALGVARDASDDDIRKAYRKLARKYHPDVSKEADADA
                     KMRAINEARDVLQDKEKRAAYDQLADHVARGGSPDGHFRQPPPGWDEGFEFHRGPGQG
                     PADHADFSEFFSSIFGNRGGMGGGMGGAPRGPYRERGEDHHAAIEISLEDALHGAERQ
                     LSLRAQEIDEQGRPHLVNRTLDVKIPAGIRPGQYIRLAGQGLPGHGGEPAGDLYLEVR
                     IAPHKRYRVEGSDLYMTLPVTPSEAALGAHVTVPTPSGAHVDVTIPKGARNGMKLRLK
                     ERGLGGKTPGHLYLLIEIALPPADNDAARAAYEALARATPSFDPRMNLGA"
     gene            complement(9339..9842)
                     /locus_tag="VIN75_23570"
     CDS             complement(9339..9842)
                     /locus_tag="VIN75_23570"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HEY8977213.1"
                     /translation="MKLPRDAVVFANPLLLWSTFGLKMLQMSTAAAQVIALRTTRMAA
                     HGLNPNAADRREMHRMGAEKVDAFSRAGTALATGAMPLLFGMAMQAWRSSFDLLAAST
                     QLAASRSIPQTMARQQQLAGTLMRGATAAGRHATSAATARLAHRALAPVHRKATANAK
                     RLARKAR"
     gene            complement(10001..10414)
                     /locus_tag="VIN75_23575"
     CDS             complement(10001..10414)
                     /locus_tag="VIN75_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977214.1"
                     /translation="MSPTRILAAAALLALGTAAFAAAPASDAASAPQRRATLAARANH
                     HLQQDAVPSVKTDPDGSLAVRADADLASRAADDRRPRLHVERQNLKGPEAAKAIAQQT
                     NPNTIDHDTAFGDKDHTQQVTNLKVTLKKKGEPAH"
     gene            complement(10533..>11687)
                     /locus_tag="VIN75_23580"
     CDS             complement(10533..>11687)
                     /locus_tag="VIN75_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8977215.1"
                     /translation="PPLEAGMPRPPRHPLVTLPNGEPLPLVVPEPRPPRGERPPSTVM
                     PHPGMRGVPYHMALNNEVDPTMWRVVRRLPDGHRIVFSLADTSHEQRAEHIGWATLAV
                     LLLLTALAYAGVRHMLSPLAALRAGAIRFGQGDFSQPIVPRNRDELGDLAEQINAMAA
                     RLHHMLDAKRQLLLAISHELRSPLARARMNAELVDESTERSALLRDLGEMRDLITDLL
                     ESERLADVQAGGHAALHTEATDLSELAREQCDAQAASGAVTLALADGLPSLPLDRARV
                     RLLLRNLVDNALRHGAESEQPPRVATVLTPATVQLVVRDFGPGMDDSQLRHAAEAFYR
                     ADAARLRSTGGVGLGLYLCRLVAEAHGGTLSLRNAHPGLEITVELPLPAA"
BASE COUNT         1571 a         4044 c         4305 g         1767 t
ORIGIN      
        1 ctaggagtct gcgcggcaga aggcgcctgc tgcgaacggc gataagcggg gccttttcag
       61 cggcggctcc tctcgcgtag cgcccactac gagttcgtcg ccgccgctga aaagtccatg
      121 cttctcgccg ctctcgcgac ggcgccttct gccgcgcaga ctcctagttg ccgctcgccg
      181 ggcggagtcg gatactggcg ccatggccat cccgcatgca agctccggac aggtcgtcga
      241 gatcctgtcc agcgacggca gcctgccgtc cgaccagtcg tacgccgtct tcaagagcga
      301 ccagctggag gtcatgcgcc tcgtcctgcc cgcgggcaag gcgtttccgc cgcaccgcgt
      361 ggccggcgag atcacggtgc aatgcctctc gggcagcctc gagctcaatg tcgacggcac
      421 gcgccaggtg ctgcgcgccg gccagctgat gttcctgcgt gggggcgtcg aacacgggct
      481 ggtgggcatc gaggaggcga tcgccctggt caccatcgtg ctgtgcaagc cttgatacag
      541 acacgttctt ctctttgtcg cgctgcgggc gtataacggc cccgtcgagc ggacgcatcg
      601 atccgccgac ccatgcgatc acgaaaagag gagcgagcat gaagcaatat ggcgccgagt
      661 tcatcggcac gttctggttg gtcctggggg gctgcggcag cgcggtgctg gcggcggcgt
      721 tccccggcgt gggcatcggc ctgctgggcg tctcgctggc cttcggcctg accgtgctga
      781 ccatggcctt cgcgatcggc ccgatctcgg gctgccacct caacccggcg gtgtccgtgg
      841 gcctgtgggc cgcgggccgc ttcccggcca acaagctgct gccctacatc gtggcgcagg
      901 tgctgggcgc gatcgtcgcc ggcggcatcc tgtacgtgat cgccagcggc gcgccgggct
      961 tcgacgtcgc caagggcttc gcgtccaatg gctacggcgt gcgctcgccg ggcggctact
     1021 cgatgctggc cggcctcgtg tgcgaggtcg tgatgacggc gttcttcctg atcgtcatcc
     1081 tgggctcgac cgacggccgc gcgtccgcgc agttcgcgcc gatcgccatc ggcctggcgc
     1141 tgacgctgat ccacctggtc agcatcccgg tgaccaacac ctcggtcaac ccggcgcgct
     1201 ccacgggcgt ggcgatctac gtcggcgccg cggcgctgga gcagctgtgg ctgttctggg
     1261 tggcgcccat cgtcggcgcc tgcctgggcg cgcagatctt caaggcgatc cggcccaccg
     1321 agatctgagc gtctggtaac ggcgggcgcg agcggacggc tgagtgcggc ggctcagtgc
     1381 cgcatggaat cgggcgaggg ccggcgcggc atggcggcac tgtacgatgg agcgcccacc
     1441 ctccctggtt cgcgccatgc agatccgccc cttccgggat tccgacgaag ccgccgtcat
     1501 cgcgttgtgg gaggccgcgg gcctcacgcg gtcgtggaac gatccgcgca aggacatcga
     1561 gcgcaagcgc gcggtgcagc gcgagggctt cctggtgggc accgtcggcg acgtcgtgat
     1621 ggcgtcgatc atgatcggct acgacggcca ccgcggctgg atcaactacc tcgcggtcga
     1681 tgccgcgcac cgcggccagg gccacgcgcg cacgctgatg cgcgaggccg agcggctgct
     1741 ggaggcggcc gggtgcccga agatcaacct gcagatccgt tcgagcaacg cgtcggtgat
     1801 cgcgttctac cagcgcatcg gctacctgca ggacgatgtg gtcagcttcg gccgccggct
     1861 gatcgccgac gagtaggcac aagcggagtc gcggaatcca ccccgaccga agcgcattgg
     1921 cgcgcgtggg tccctgcgac tgcgcgcagg ccgctactcg cttcccgcta ctcgctgttg
     1981 ccctcgatgc gacgcgcccg cacgcgcttg cccttgacga agcccgcgtt gagcttggcc
     2041 agcaccttgt cggcgatcgc gcgctcgacc gcgacgtagg tcgacatgtc ggtcacgttg
     2101 atcttgccga tcttcgcgcc ttcgacaccc atgtccttgg tcagggcgcc cagcacgtcg
     2161 cccgggcgca tcttctcctt gcggccgccc aggatctgca gcgtcaccat cggcgcgacc
     2221 agcggctggt cgtcgcgggc gtccagctcc gacagctcgt gccattccgt ctcgcccttc
     2281 tgcagcacct cgatgttgcc gacgcggccc atctcgtcga ggctggccag gttcagcgcg
     2341 aggccgtcct cgccggcccg gccggtgcgg ccgatgcggt ggatgtgctg gtcgggctcg
     2401 taggtgacgt cgacgttgac gacgcaggcg agctgcggga cgtccaggcc gcgcgcggcc
     2461 acgtcggtgg ccaccagcac cgagcagctg cggttggcga actggatcag cacctggtcg
     2521 cggtcgcgct gctcgaggtc gccgtgcagc gcgatggcct ggatgccctc ggcggtcagg
     2581 acgtcgacca ggtcgcggca ctgctgcttc gtgttgcaga acgcgatcgt gttggcgggc
     2641 tggtagtggc gcagcaggcg gcccacggcg tgcaggcgat cctgctcctc gacctcgtac
     2701 cagagttgca cgatctgcgc ggcggagtgg cgcgactcga ccttcaccat ctgcgggctg
     2761 cgcatgaact gctggctcag cctggcgatg ccctcggggt acgtcgccga gaacagcagc
     2821 gtctggcgcg tcttggggca ggtgccggcg accgccttga tgtcgtcgac gaagcccatg
     2881 tccagcatgc ggtcagcctc gtcgagcacc agcgtcttca ggccggacag gtccagcgcc
     2941 tcgcgctcga gcaggtccag cacgcgcccg ggcgtcccga tgacgacgtg cgcgccgtgc
     3001 tccaggctcg cgagctggtt gcgcaccggc acgccgccca ccaccgtcag caccttgatg
     3061 ttctcctcgg cgcgcgccag ccggcgcgtc tcgacggcca cctgctcggc gagctcgcgc
     3121 gtcgggcaca gcaccagcgc ctgcacgccg aagtggcgcg ggttgagctt gtcgagcatc
     3181 gcgagcgcga aggcggccgt cttgcccgag cccgtcttgg cctgcgcgat gatgtcctgg
     3241 cccgccagcg ccgggggcag ggcggcggcc tggatgggcg tcatctcgac gtagcccagc
     3301 tgcgcgaggt tggccagcac gttcggcggc agcgcgaggg tgtcgaagga ggaggcgggc
     3361 gcgggcgagt tcatcgggcg attgtcggcg atgcgtcgtc tcgcggacat gcggcgcgga
     3421 gtaggatcgg cgcgcaccgc cccgcccgac cgccgagatg accgccgccg accgccagtc
     3481 cagccccgcc gccgaacgca accgccagcc gatcctgcag gtgctgcgcc gcgtgctgcc
     3541 gcccgccggc cgcgcgctcg acatcgccag cggcagcggc cagcacgtgt cgtggttcgc
     3601 gcgccacctg ccgggctgga cctggcagcc cagcgagtac gcggccgcgt cgctggcgtc
     3661 gatcgcggcc tggagcgtgc tggacgaggc cgatctcgcc gagtcgccgc aggcggtcgc
     3721 cggcaccggc ccgctcgcca acatcgcgtc gccgctgcaa ctggacgtct gctcgcccga
     3781 gtggccggtc agcggcgtat tcgacgccat cacctgcatc aacatgctgc acgcatcgcc
     3841 gcccgcgacg ctgccgggcc tgatgcaagg cgtcggccgg cacctcgcgc gcgggggcgt
     3901 gctcgtcacc tacggcccgt acatcctcga cgacgagccg ctcgcgccca gcaacgtcga
     3961 gttcgaccgc tggctcaaga ccgagcgcga cccgagctgg ggcatccgcc gcctggccga
     4021 cgtcgccgcg caagcgcacg acgcgggcct gcgcctgcgt gagcgcgtcc cgatgcccgc
     4081 caacaacctc gtgctggtct tcgaccgcga aggagcctga catgaccaac acgcaagacg
     4141 cctcgaccga cggcgtgccc gccgaactgg ccatcgccga gacccgcgcc tgggtgcgcc
     4201 gcgccgtgat cggcctgaac ctgtgcccgt tcgcgcgcgc cgtcgacgcc aaggaccaga
     4261 tccgctacgt cttcagcgac gcgaccgatc ccgagacgct gctcgccacg ctcgtggtcg
     4321 aactgcagcg gctggccgac accgaccccc aggtcgtcga cacgaccatg ctgatacacc
     4381 cgcgggtatt ggcggacttc gaggacttca acgatttcct ggagctcgcc gacgccgcgg
     4441 tcgaggatct cgacctcgac ggcgtcctgc aggtcgccag cttccacccg cgcttccagt
     4501 tcgccgacac cgagcccgac gacatcacca acgcgaccaa ccgctcgcca taccccaccc
     4561 tgcacctgct gcgcgaggac agcgtcgacc gcgccgtggc cgcgttcccc gaggccgagg
     4621 ccatcttcga gcgcaacatg gagacgctgg acaagctcgg cgccaagggc tgggccgacc
     4681 tccgcaagag ctgggaagac gacgccgccg ccgaactggg aagttgaggc gaaagagccc
     4741 cttcagcttt gcaagttttg taaccccgcg gcacactggg gccatggccc ctccagctcc
     4801 atccccggtc gtcgcgtcgc tgctggtggt cgacgacaac gtcgtccagc gcatccaggt
     4861 ggtggcgctg tgccgcgagc tgggcatcga catgatctac gaggcgggca gcggggacga
     4921 ggcgctggag ctgctgtcgc tgctcgtgct gccgcccgac gtggcgatcc tcgacctcga
     4981 gatgcccggg atggacggca tcgagctgat cgagcgcctg cacgagcgca agctcgcgat
     5041 cccgctcgtg gtcgtgtcgg gccgcgaggc ggtgctgatc gacaccgtcg aggcgatggc
     5101 gcgcaacctc ggcatgccgg tggtggcggg catccgcaag ccgctgacca gggaggcgct
     5161 ggcggcggcg ctcgacggct tggagcgcga gcgcaccggc ccggtcacgg ccgccgagcg
     5221 tgcggcgctg ccgccgatcg acccggccga tctcgccgcc gcgatcgcct cgggcgggat
     5281 cgccgtgcac taccagccca aggtcgacat ggcgcgcggc gccgtgtgcg gcgtcgaggc
     5341 gctcgcgcgc tggacgcatc cgcggctggg cccggtcggc cccgatcgct tcatcgaggt
     5401 ggccgagcgg gaaggcctga tccacgcgct gacgctggcg gtgctgcgcc aggccttcac
     5461 ggacgccgcg cgctggcgca cgggcggcct gcacctgtcg ctggccgtca acctgtcgcc
     5521 gcggctgctc gacgacccgt cgctcgtgcg cgagctctcg caactgctcg acgaccacgg
     5581 cctggacgcg cgccaggtcg tgctggagat caccgagaac tcggtcgtcg actgcatggg
     5641 cgtggcgctc ggcgttcttg cgcgcctgcg gctgaagggc ttccggctgt cgatcgacga
     5701 ctacggcacg gggttctcgt cgatgcagca gctcgcgcgc gtcccgttca ccgagctcaa
     5761 gatcgatcgc gccttcgtgc acggcgcggc gcagcgcacc aacctgcgcg tgatcctgaa
     5821 gtcggcgctg gacatgtcgc gccagctggg cctggtgacg gtggccgagg gcatcgagac
     5881 gctgcccgac tggcagctcg tgcgcgactc cggctgcggc atcggccagg gcttcatcgt
     5941 gtccagggcg atgccggccg acgagctgcc gcgctggctc gaggcgcacg aggcgcggtt
     6001 gcgggagctg ggaacgggcg agtagcgcgg cgcgtcagtc cgtccgcttc aggaggacct
     6061 tgaacatcgt cgtccacccg tcgggcccct gcgcctcgct ccaggcctcg taggccttgt
     6121 cgccctggcg cgtccagcgg gcgcggtaga cgagcgcctc gccgtcggag acgtattgcg
     6181 tgtcggggaa cagcagcgcc tcgggcaggc tctcgacggt gccgcggctg gagccgccac
     6241 cgctttcgat gtacaggaac gcgacgtggt tgccgacggt gtcgacgaag tacgtggact
     6301 cgcccgtcat gtcgggcttg ccggggcggc gcacggtgtg ggtgtcgcgc agcacgctgc
     6361 cgccgtacat ccagaagaag cagtgctcgt cggtcgcctt gccgtccggg aacgtgcctt
     6421 tccagcaatg ccccgcgagg aaggccatcg gcttgagcag ttccgccgtc gccgccgcgg
     6481 ccgtgccggc gaaggtggtc agggcgaggg cggcgagaag gcggcgcatg cgggagctcc
     6541 ggtggactgg cgctcgcgcg ggcgcgcgag gccagtgcag tctagcgaca aagccggcgc
     6601 ccggatcgca tgcgtcacgc gatgtccgcg cggcgccgtg cgcgcagccc gatcacctgc
     6661 agcgtggcgc cggccaggaa cagcaggaac cagacgccca gcgtgccctt gacgatggcg
     6721 gacatcggcc cgtcggcgat cggctgcgac ggcggcagcc gcgtgagcgt ctcgttgatg
     6781 ccgggcacca gcagcaggaa gaagctgaac gacatcgcga gcgtggcgag gtagtcgcgc
     6841 gtccggccca gcgcctcgat gcggggcacg agccaggcgc cggcgagggc gagcagggcg
     6901 aggatgccca gcgcgtggcc ggggttgaag ccgccggtgc tcgagagccc gaaggacgtc
     6961 agcaccgacg ccaccaatcc ggcgacgtac acgcgtcccg cggtggaccg gggctcgatg
     7021 cggcgatgga tcgccaggct gcacagcccc gccacgagcg gcaccaggct gatggcggta
     7081 tgggcgatgc ccagggtcga gagcggatgg ggcatggtgt ggtccttgat gtatatctac
     7141 tagttggtag actgcgaggt caaaaaaaag cccggcgact caatggtgcg tcaggcgatg
     7201 cagcacgggc ttcatgatcg tgccgaacag ctcggtgtcg ccatgggcgc gcgccgcgag
     7261 catggcgccg tgcacggacg ccatgacggc catcgcctcg gccttcgccg gcccgcgcag
     7321 gtggaagacg ccgtcggcga caccgcgctc cagcacggcc tggagccagc cggccaggtc
     7381 gtcgaagtgt ccacggactt cctccgcgac ctcgccgggg atggctggca tctcggcggc
     7441 cagcatggcg cagacgcaga acggcgcggt gccgtcgcgg atgcaggccg tccagtagcc
     7501 cacgtacgcg tgcagctgcg cgagcgggtc ggcgatctgc tgctcgaggg cggccatgcc
     7561 ggcgcgcgcg ccgtcccggt agcgccgcac gacggtgcgc acgagatcgg ccttgctggc
     7621 gaaatgatgg tggacactgg gcttgctgat gtgcaccgcc tccgagatgt cggcatagct
     7681 gaagccgttg tagccgcgca cggccagcag cgactgcgcg caggcgacga tctcgctggc
     7741 cttcggggaa agttcaacgt ccatgccgcg cagtctacca actagtaggc ttgtgtcaag
     7801 ggccatgggt cacgcgtgcg gagaggggct gccgaagcgt cgaccgaagg gcttgtcgcg
     7861 tgacggcatg ccatcgaagg tgtccggcac ctccttggcc gcgccagggc gtcggcgatg
     7921 gccagcgtgc aggccgcgac cgaggccgtg ccagagagct gcggccggca ccgcgcaccg
     7981 gcggagactc aggacatcct gcgtccccgg cccccgcctg gccgcccgcg cgtcacgcca
     8041 gcccgtgcgc gtgcatgagc gcgcgcacgc ggcgcagttc ggactgcagg tcgaggatca
     8101 gcgccgccgt atcgaggttg gcgccgaaat cgcgttccag gcggcgggcg gccagcgcgc
     8161 gttgcaggtc ggcgctgcgg aaatgccagc gctcgggcgg gtcggcgggg gtgtcgacgt
     8221 gcaggatctc gacctccacg agccgcacga tccattggtc ctcggcgccc acggcgcggg
     8281 ccagttcgcg caacgtgagc ggcgtcgatt cgatgacggt ggtgacggcg cgcatgtcag
     8341 gctcccaggt tcatgcgggg atcgaacgag ggcgtcgccc tggccagcgc ctcgtatgcg
     8401 gcgcgcgcgg cgtcgttgtc ggccggcggc agcgcgatct cgatcagcag gtacaggtgt
     8461 ccgggcgtct tgccgccgag gccgcgttcc ttgagccgca gcttcatgcc gttgcgcgcg
     8521 cccttgggaa tggtcacgtc gacatgtgcg ccggacggcg tcggcaccgt cacgtgcgcg
     8581 ccgagcgcgg cctcgctggg cgtgacgggc agcgtcatgt acaggtcgct gccttccacg
     8641 cggtaccgct tgtgcggcgc gatgcgcacc tcgaggtaca ggtcgcccgc gggctcgccg
     8701 ccgtggccgg gcaggccctg gccggcgagg cggatgtact ggcccgggcg gatgccggcc
     8761 gggatcttca cgtccagcgt gcggttgacc aggtgcggcc ggccctgctc gtcgatctcc
     8821 tgcgcgcgca gcgagagctg gcgctcggcg ccgtgcagcg cgtcctccag ggagatctcg
     8881 atggcggcgt gatgatcctc gccgcgctcg cggtacgggc cgcgcggcgc gccgcccatg
     8941 ccgccgccca tgccgccgcg gttgccgaag atcgacgaga agaactcgct gaaatcggca
     9001 tggtcggccg gcccctggcc ggggccgcga tggaactcga agccctcgtc ccagccgggc
     9061 gggggctggc ggaaatggcc gtcggggctg ccgccgcgcg cgacgtggtc ggcgagctgg
     9121 tcgtaggccg cgcgcttttc cttgtcctgc aggacgtcgc gcgcctcgtt gatcgcgcgc
     9181 atcttcgcgt cggcgtcggc ctccttgctg acgtcggggt ggtacttgcg ggccagcttg
     9241 cggtaggcct tgcggatgtc gtcgtcgctg gcgtcgcggg cgacgccgag ggcgctgtag
     9301 taatccttga agtccatggc ggcggtgtgg gcgtggtgtc agcgggcctt cctggccagg
     9361 cgcttcgcat tggccgtggc cttgcgatgc acgggggcca gggcgcggtg cgcgaggcgc
     9421 gccgtggcgg ccgacgtcgc atggcggccg gcggccgtgg cgccgcgcat caacgtgccg
     9481 gcgagctgct gctggcgcgc catcgtctgc gggatcgacc ggctcgccgc caattgcgtc
     9541 gacgcggcca gcaggtcgaa gctgctgcgc caggcctgca tggccatgcc gaacagcaac
     9601 ggcatcgcgc cggtcgccag cgccgtgccg gcgcgcgaga acgcgtcgac cttctcggcg
     9661 cccatgcggt gcatctcgcg ccggtcggcg gcgttcggat tgaggccgtg ggcggccatg
     9721 cgcgtggtgc gcagcgcgat cacctgtgcg gcggcggtgg acatctgcag catcttcagg
     9781 ccgaaagtgc tccacagcaa gagcggattg gcgaacacga cggcgtcgcg gggcaacttc
     9841 atgggcggat ccttcgcgcg cgagcgcagg ggcgcccgga cgcgggcgcg gcggctctca
     9901 atgagcaagc gacgaacccg gattcccgtg cggaattcgg ctcgccaggc gtgcagattg
     9961 tggcgcctcg gcagctttca acgccggacg aaccgctgtt tcagtgcgcg ggctcgccct
    10021 tcttcttgag cgtcaccttc aggttggtga cctgctgggt gtggtccttg tcgccgaagg
    10081 cggtgtcgtg gtcgatcgtg ttcgggttgg tctgctgcgc gatcgccttg gcggcctcgg
    10141 ggcccttcag gttctggcgc tcgacgtgca gccggggccg ccggtcgtcg gcggcgcgcg
    10201 aggcgaggtc ggcgtcggca cggacggcga ggctgccgtc cgggtcggtc ttgacggacg
    10261 gcacggcgtc ctgctgcaga tggtggttcg cccgggcggc gagcgtcgcc ctgcgttgcg
    10321 gcgcgctcgc ggcgtccgat gcgggagcgg cggcgaaggc ggcggtgccg agggccagca
    10381 gcgcggcggc cgcgaggatg cgggtcgggc tcatgcaatt ctcccggcgg ccaggcggcg
    10441 gccgccctgt cctgtcgagg caaggcgcgc gcctgcacga ttcaagacct gacaccgcaa
    10501 ctttcaagac ctgaccccgg tcaggcggcc cgtcaggcgg cgggcagcgg cagctcgacg
    10561 gtgatctcca ggccggggtg ggcgttgcgc agggacaggg tgccgccgtg ggcctcggcg
    10621 accaagcggc acaggtacag gccgaggccg acgccgccgg tgctgcgcag gcgggccgcg
    10681 tcggcgcggt agaacgcctc ggcggcgtgg cgcagctggc tgtcgtccat tccggggccg
    10741 aagtcgcgca ccaccagttg caccgtggcg ggcgtgagca ccgtcgcgac gcgcggcggc
    10801 tgctccgact cggcgccgtg gcgcagcgcg ttgtcgacga ggttgcgcag cagcagccgc
    10861 acgcgggcgc gatcgagcgg cagcgacggc aggccgtcgg ccagcgcgag cgtcacggcg
    10921 ccggaggcgg cctgggcgtc gcactgctcg cgggcgagct ccgacaggtc ggtcgcctcg
    10981 gtgtgcagcg ccgcgtggcc gccggcctgc acgtcggcca ggcgctcgct ctcgagcagg
    11041 tcggtgatga ggtcgcgcat ctcgcccagg tcgcgcagca gcgcgctgcg ctcggtgctc
    11101 tcgtcgacca gctcggcgtt catgcgggcg cgcgcgagcg gcgagcgcag ctcgtggctg
    11161 atggccagca gcagctggcg cttggcgtcg agcatgtggt gcaggcgcgc ggccatcgcg
    11221 ttgatctgtt cggcgagatc gcccagctcg tcgcggttgc gcggcacgat gggctgcgag
    11281 aagtcgccct ggccgaagcg gatcgcgccg gcgcgcagcg cggccagcgg gctgagcatg
    11341 tggcgcaccc cggcgtaggc gagcgcggtc agcagcagca gcacggccag cgtggcccag
    11401 ccgatgtgtt cggcgcgctg ctcgtgggac gtgtcggcca ggctgaagac gatgcgatgg
    11461 ccgtccggca gtcgccgcac cacgcgccac atcgtgggat cgacctcgtt gttgagcgcc
    11521 atgtggtacg gcacgccgcg catgcccggg tggggcatga ccgtcgacgg cggccgctcg
    11581 ccgcgcggcg ggcgcggctc cggtaccacc agcggcaacg gttcgccgtt cggcagcgtg
    11641 acgagcgggt gccgcggcgg ccgtggcatc cccgcctcca gcggcgg
//
LOCUS       DATCRO010000866         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_54791, whole genome shotgun sequence.
ACCESSION   DATCRO010000866 DATCRO010000000
VERSION     DATCRO010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54791"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VIN75_23585"
     CDS             <1..608
                     /locus_tag="VIN75_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEY8977216.1"
                     /translation="MFSRDEPRTGTAGTLAWAVDVDNPATGETFTLTMKEVTLRSAAA
                     ANAPAGAAPTLVTRPCAVGFSGNYPRALDGLARLLSLDMRVIDAAWIGMKLRKLLNVG
                     EPLGHFMAPVPGHKRQQVWPSTIAYVARLMIHRYAMLGVLDEDGFPLDSTGLLEPPTP
                     RPTGQLAPQAGRPCPECGNATMIHKDGCDFCTSCGYVGACG"
     gene            826..2154
                     /locus_tag="VIN75_23590"
     CDS             826..2154
                     /locus_tag="VIN75_23590"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY8977217.1"
                     /translation="MHSSPHDASGGPLRVGLLGIGVVGTGVHRLLQRNRALIERRAGR
                     AIELRAIAARDVARARAVAGLDAPVVGDAMAVATAPDIDVVVEVVGGCMAARELVMAA
                     IAHGKHVVTANKALLALHGEEILAAAHEQGVVVAFEGAVAVSIPIVKALREGLAANEV
                     AWLAGIVNGTSNFILTQMRERAASFGDALREAQRLGYAEADPALDVDGIDSAHKLALL
                     ASLAFGMRLSFDQIEVDGIRGVQLADLQCAERLGHRVKLIASARRHGAKVELRVGPAL
                     VPAGSLMAGIGGSMNGIVVHGDAAGPTMYCGAGAGSLETASAVVADLVELARAARVPA
                     RDRVPALGLPRDQAVTLPSVASRDVSTPHYLRMTLAQGADLARVLDVLAGACLQVAAH
                     VDVAAPDGARGLALLTRAAPKSALIEAVGLLERRGLVLAGAVGLRVEALQ"
     gene            complement(2239..2724)
                     /locus_tag="VIN75_23595"
     CDS             complement(2239..2724)
                     /locus_tag="VIN75_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977218.1"
                     /translation="MSIAPLSTTSSATASNSGRTELSEHIGRAQPAAVASPGSTMAGI
                     VGGVAAGVSATVAFSGEALHALEQAGEFVVGGAEDLAVGAWHGLEHGAQAVAQAGEAI
                     ADTVETGAKEIAETAVAAGKQLAHYAAVGAHATGEAVSDVASGTVMAASAAGKTIMAL
                     I"
     gene            complement(2883..>3656)
                     /locus_tag="VIN75_23600"
     CDS             complement(2883..>3656)
                     /locus_tag="VIN75_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977219.1"
                     /translation="PLLPRGTRLAARHLPLLAGGGQATASVLARPRVGVLALACADAS
                     PPRARTGAAASAAWVAATVEQLGAQPFETTCRPPTPRRFVQTLEMFIGECALVVVLGF
                     LDPAQCEALRQAQARLGQEPCVDVMRPRPFGALQVARLGTTLVVALPADLEGAVAAFT
                     TLLAPLVRRLQGRHDALPEARAAELQVPPRRDDERWRLFPVRIDPGALNSRMRLLPCR
                     GDDAATALAIADGLAWHAAEFSSFERPTVAFFPFEGAAG"
BASE COUNT          468 a         1340 c         1372 g          476 t
ORIGIN      
        1 cgatgttcag ccgcgacgag ccgcgcaccg gcaccgccgg cacgctggcg tgggccgtcg
       61 acgtcgacaa cccggccacc ggcgagacct tcacgctgac gatgaaggag gtgacgctgc
      121 gctcggccgc cgccgcgaac gcgcccgcgg gcgccgcgcc cacgctcgtc acgcgtccgt
      181 gcgcggtggg cttctcgggc aactacccgc gcgcgctcga cggcctggcg cgcctgctgt
      241 cgctggacat gcgcgtgatc gacgccgcgt ggatcggcat gaagctgcgt aagctgctca
      301 acgtcggcga gccgctgggc cacttcatgg cgccggtgcc cggccacaag cgccagcagg
      361 tctggccgtc gacgatcgcc tacgtcgcgc gcctcatgat ccaccgctac gcgatgctcg
      421 gcgtgctcga cgaggatggc ttcccgctcg actcgaccgg cctgctcgag ccgcccaccc
      481 cgcgcccgac cggccagctc gccccgcagg ccggccgccc gtgcccggag tgcggcaatg
      541 ccaccatgat ccacaaggac gggtgcgact tctgcacgtc gtgcgggtac gtgggcgcgt
      601 gcggctgaac gtgacggacg gccgcgccgg cttgacgccc gtcgcggccg catggcacga
      661 tccgtccatg tccttccgga tctcgactcc cattaccacc accaccgcgt tgcgcggcgc
      721 gtggaggtaa ggcttcgtcc gaagacactt catcccccag gccccgccag cgatggacgg
      781 ggcttttcgt ttctccgtcc cctcgcggct catcacggag aaaccatgca ttcctcacct
      841 catgacgcgt ccggcggccc cttgcgggtc ggcctgctgg gcatcggcgt cgtcggcacg
      901 ggcgtccatc gcctgttgca gcgcaatcgc gcgctgatcg agcggcgcgc cggccgggcc
      961 atcgagctgc gcgcgatcgc ggcgcgtgac gtcgcccggg ctcgtgccgt cgcgggcctg
     1021 gacgcgcccg tcgtcggcga cgcgatggcg gtcgcgaccg ctccggacat cgacgtggtg
     1081 gtggaggtcg tcggcggctg catggcggcg cgcgagctcg tgatggcggc catcgcgcac
     1141 ggcaagcacg tggtcacggc caacaaggcg ctgctggcgc tgcatggcga ggagatcctc
     1201 gcggccgcgc acgaacaggg tgtcgtcgtg gcgttcgagg gcgccgtcgc cgtgagcatc
     1261 cccatcgtca aggcgttgcg cgaagggctg gcggccaacg aggtcgcgtg gctggcgggc
     1321 atcgtcaacg gcaccagcaa cttcatcctg acgcagatgc gcgagcgcgc cgcgagcttc
     1381 ggcgatgcgc tgcgcgaggc gcagcggctt ggctacgccg aggccgaccc ggccttggac
     1441 gtggacggca tcgacagcgc gcacaagctc gcgttgctgg caagcctggc gttcggcatg
     1501 cggctgtcgt tcgaccagat cgaggtcgac ggcatccgcg gcgtgcagct ggccgacctg
     1561 cagtgcgccg agcgcctggg ccaccgcgtc aagctgatcg ccagcgcgcg gcggcacggc
     1621 gcgaaggtgg aattgcgcgt cggcccggcg ctggtgcccg cgggcagcct gatggccggc
     1681 atcggcggct cgatgaacgg catcgtcgtc catggcgatg ccgccgggcc gacgatgtat
     1741 tgcggcgccg gcgcgggctc gctggagacg gcgtcggccg tggtggcgga cctcgtggag
     1801 ctcgcgcgcg cggcgcgggt gcccgcgcgc gaccgcgtgc ccgcgctggg actgccgcgc
     1861 gaccaagcgg tgacgctccc gagcgtggcc tcgcgcgacg tgtccacgcc gcactacctg
     1921 cgcatgaccc tggcccaagg ggccgatctc gcgcgcgtgc tcgacgtgct ggccggcgcc
     1981 tgcctgcagg tcgccgcgca cgtcgacgtc gcggcgccgg acggcgcgcg gggccttgcg
     2041 ctgctgacgc gggcggcgcc gaagtccgcg ctgatcgagg cggtcggcct gctggagcgc
     2101 cgcggcctgg tcctggccgg cgcggtgggc ctgcgcgtcg aggcgctgca gtaggcgcgc
     2161 ccgatcgaga caaggcgcaa ggcggcgctt tcagccgcga cggccgctgg gagcgctcgt
     2221 ccggccgcgc gcggccggtc agatcagcgc catgatcgtc ttgccggccg cggaggccgc
     2281 catcacggtg ccgctggcga cgtcggagac ggcctcgccg gtggcatgcg cgccgaccgc
     2341 ggcgtagtgc gccagctgct tgccggcggc cacggcggtc tcggcgatct ccttggcgcc
     2401 ggtctcgacg gtgtcggcga tcgcctcgcc ggcctgcgcg acggcctgcg cgccatgctc
     2461 cagcccgtgc caggcgccga ccgcgaggtc ttccgcgccg ccgacgacga actcgccggc
     2521 ctgctccagc gcgtgcagcg cctcgccgct gaacgccact gtcgcgctga cgccggcggc
     2581 gacgcccccg acgatcccgg ccatcgtcga gccggggctc gccacggcgg ccggctgggc
     2641 gcggccgatg tgttccgaca gctcggtgcg gcccgaattg ctggcggtgg cggacgatgt
     2701 cgtggagagc ggcgcgatgg acatgctgtg gatcccgatg ctcggccgtg aacggagact
     2761 ggccgatgag tggatcctag agatccgcac gtcgtgcaag ggcccgatca agcgggcaaa
     2821 acaggccggc gatacgtcgc caaacaatgt gtcaggaccg ataccgcctg ctcagcatgc
     2881 gttcagccgg cggcgccctc gaacgggaag aaggccactg tcgggcgctc gaagctcgag
     2941 aactccgccg cgtgccaggc caggccgtcg gcgatggcca gcgcggtggc ggcgtcgtcg
     3001 ccgcggcagg gcagcaggcg catgcggctg ttcagcgcgc cggggtcgat gcgcacggga
     3061 aacagccgcc agcgctcgtc gtcgcgtcgc ggcggcacct gaagctcggc cgcgcgggcc
     3121 tcgggcagcg cgtcatgccg cccctgcagg cggcgcacca ggggggccag cagcgtggtg
     3181 aaggcggcga cggcgccctc gaggtcggcc ggcagcgcga cgacgagcgt cgtgcccagc
     3241 cgcgcgacct gcagcgcgcc gaacggccgc ggccgcatca cgtcgacgca cggttcctgg
     3301 ccgagccgcg cctgcgcctg ccgcagcgcc tcgcactgcg ccggatcgag gaagccgagc
     3361 acgacgacga gcgcgcattc gccgatgaac atctccagcg tctgcacgaa gcggcgcggc
     3421 gtgggcgggc ggcacgtcgt ctcgaacggc tgcgcgccca gctgttcgac cgtggcggcg
     3481 acccaggcgg cgctcgcggc cgcgcccgtg cgcgcacgcg gcggcgacgc gtcggcgcag
     3541 gccagcgcca acacgcccac gcgcggccgc gccagcaccg acgccgtcgc ctggccgccg
     3601 ccggccagca gcggcaggtg gcgggcggcc aggcgcgtgc cgcgcggcaa cagggg
//
LOCUS       DATCRO010000867         6577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_831235, whole genome shotgun sequence.
ACCESSION   DATCRO010000867 DATCRO010000000
VERSION     DATCRO010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831235"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VIN75_23605"
     CDS             <1..183
                     /locus_tag="VIN75_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY8977220.1"
                     /translation="CVLREGSKLIRSILFDYTRLSQGETLEDMIVSPHYCVDRHGNTT
                     YPGDEGTVVRWGDARA"
     gene            257..1702
                     /locus_tag="VIN75_23610"
     CDS             257..1702
                     /locus_tag="VIN75_23610"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY8977221.1"
                     /translation="MAASIPIQPVIMAGGSGTRLWPLSRSGFPKQFLSLGSDDSLFQQ
                     AIARLVGLADGDIAVEAPLIVGNAEHRFLALEQMREMRVEPAAVILEPVGRNTAPALT
                     LAALAAREGGADPVLVVSPADQTITDRAAFTAALRAAVHGAAGGAIVILGITPTSPET
                     AYGYIRSTPADGLHKVAQFVEKPDAATAARYLADGGYTWNSGMFVLRASVWLDALREF
                     RPDILAATEAAWSGRTPDERFVRPTREAFLAVPSESVDYAVMEKCPGSRFDIRMVPLD
                     AGWNDLGAWDAVWQVAAKDAAGNSGVGDVIFEDSRNTLVHASGRLVTTLGVDNVVVVE
                     TADAVLVADRQRSQDVKKLVSRLDKDRRTEHAMHRKVHRPWGWYDSVDAGPRFQVKRI
                     MVNPGATLSLQMHHHRAEHWIVVQGTAEVTCGDRKMILTENQSTYIPLGETHRLANPG
                     KLPLEIIEVQSGSYLGEDDIVRFEDTYGRTS"
     gene            1764..3158
                     /locus_tag="VIN75_23615"
     CDS             1764..3158
                     /locus_tag="VIN75_23615"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY8977222.1"
                     /translation="MFEERSVRKQFLSAPPSVGSTIASLLEPLVAAGMLALFHELWGY
                     RMDGVAMALAILLLVLMFPGVNRFGRTGVGVGIDIILSWVWVLSVLALLGYATNTLAS
                     FDTDMLIAWGIATPLVQWALVAIGTAIQRHLASLPDARRPAVIIGAGRMGMRVAQMLK
                     LRQAFGHDLLGVFDDRSADRVNLPEEAVLLGTLQQLPEYIEAHGVKDVFITLPLTSQP
                     RIQSLLESLQNTTASIHFVPDIFGVSVIQGRLEDMGGVPVVGLMVAPFTGINGFIKRV
                     SDIVLSTLILIMISPILLVLAIGVKMSSPGPIIFRQRRTGLDGEIIEVYKFRSMRTTE
                     DGAVVKQATRDDPRITPFGAVIRKTSLDELPQFVNVLQGRMSIVGPRPHAVAHNEQYR
                     KLVKAYMARHKVKPGITGWAQVNGLRGETDTLDKMAARVEYDLEYLRNWTLGLDLLII
                     ARTVKLVFLDRSAY"
     gene            3287..6286
                     /locus_tag="VIN75_23620"
     CDS             3287..6286
                     /locus_tag="VIN75_23620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEY8977223.1"
                     /translation="MTILADAEPSALAPVAAPAASAAAAVDVAVAATFTAEGLEAPLA
                     FMLDAAGLAGRIAFAPYHQLFQELLTPGSALARNAAGGGIGVVALRLEDFVRDAEAGA
                     DLAALVSRVAGELADAFRQFRQRASAPLLVFVLRASPALDRGLAAVVDAQSALLSAAL
                     EALPDTAVFGDAAVDALCPATRHDPVADKLAHIPFTDDWFAALAIVVARQVHMRKVAA
                     HKVLVLDCDNTIWRGVVGEDGVAGIALDPGFLAVQDYAVAAQSRGVLVCLASKNAEAD
                     VMRVFDERPDMRLARGHVVAHRINWELKSANLRALARELNLGLDAFVFLDDNPVECGL
                     MQEMLPEVVTLQLPSPEAAESLLANLWAFDKVSVTQEDQRRTEMYRQNAEREQLEASA
                     TDIGAFLASLELKIDVSAPAEGDWPRLSQLTQRTNQFNFTTRRRGEPEMRALGGSTTV
                     QAVRVSDRFGDYGLVGLVIADETVDAIEVDTLLLSCRVLGRGVEHAMLASLGRLAQSR
                     GKALVRLHLVRTPKNEPALAFADAVVGAYRREVEDGVAWDVPADVAAAIVHRAGEDAP
                     EVIAARKADAAKAPKAAAPSLRGRSARYARLATELTTGPAVMQALRAATRAVRAPAAA
                     SAQPRTATERALVVLWEELLGVSGLGIDDDFFALGGTSLLAARMIAELAHRRGVALPL
                     TTIVEAPTLRQFAQRADQGAAAAPNRLVTLRESGERRLFLVHDGDGETLLYRNLANRL
                     PATLSVYGIQPERRPRIPLAHLSIEEMAASYVKTVRAVQPDGPYLLGGMCAGGLIAFE
                     MARQLQADGAVVERVLMMDSATPQAERRDIENAQRSSRLREAVAAAREGRGVVGGLVA
                     AAGVVARKVFGRVAWELRKRRLQSDDDRRLATLRQVLASGSEWPASEPSLDFRAIYNH
                     AEHAYRPPPSAISAVLLRATAGSGNDIPYREVFADPTLGWAGVARDLVVVDVEGGHAS
                     MLQEPQAAALARAILPHVAGAAMRAAA"
     gene            6345..>6577
                     /locus_tag="VIN75_23625"
     CDS             6345..>6577
                     /locus_tag="VIN75_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977224.1"
                     /translation="MSAGASLATMTVGIVVVSYKSAALTVDCLRSIAAERAGPGPRIR
                     CVVIDNASGDAPAVAAAIAEHGWGDWARVVVAPR"
BASE COUNT          906 a         2414 c         2332 g          925 t
ORIGIN      
        1 tgcgtgttgc gcgagggcag caagctcatt cgcagcatcc tgttcgacta cacgcgcctg
       61 tcccagggcg agacgctgga agatatgatc gtgtcgccgc actactgcgt cgaccggcac
      121 ggcaacacca cgtatcccgg cgacgaaggc accgtcgtcc gctggggcga cgcacgcgcc
      181 tgattcgcct gaaaggcagc cgccgcgaca ccggccgtgt gtcgcgcctg cctctttcct
      241 tgacgaaagc gtcctcatgg cagcgtcgat cccgatccaa cccgtcatca tggccggagg
      301 cagcggcacg cggctgtggc cgttgtcgcg ctcgggcttc cccaagcagt tcctgtccct
      361 gggcagcgac gacagcctgt tccagcaggc catcgcgcgc ctggtgggcc tggccgacgg
      421 cgacatcgcc gtcgaggcgc cgctgatcgt cggcaacgcc gaacaccgtt tcctcgcgct
      481 ggagcagatg cgcgagatgc gcgtcgagcc cgcggccgtc atcctcgagc ccgtgggccg
      541 caacaccgcg cctgcgctga cgctggccgc gctggcggcg cgcgagggcg gggccgaccc
      601 ggtgctggtg gtgtcgccgg ccgaccagac catcaccgac cgcgccgcgt tcaccgccgc
      661 cctgcgcgcc gcggtgcatg gcgccgccgg cggcgcgatc gtgatcctgg gcatcacgcc
      721 caccagcccc gagacggcct acggctacat ccgcagcacg ccggccgacg gcctgcacaa
      781 ggtggcgcag ttcgtcgaga agcccgacgc ggccaccgcg gcgcgctatc tcgccgacgg
      841 cggctacacc tggaattccg gcatgttcgt cctgcgcgcc tcggtgtggc tggacgcgct
      901 gcgcgagttc cggcccgaca tcctggccgc caccgaggcc gcctggagcg gccgcacgcc
      961 cgacgagcgc ttcgtgcgcc cgacgcgcga ggcattcctg gccgtgccgt ccgagtcggt
     1021 cgactacgcc gtcatggaga agtgcccggg cagccggttc gacatccgca tggtgccgct
     1081 ggacgccggc tggaacgacc tgggcgcctg ggacgccgtc tggcaggtcg cggccaagga
     1141 cgccgccggc aactcgggcg tcggtgacgt catcttcgag gacagccgca acacgctggt
     1201 gcacgccagc ggccgcctgg tcacgacgct gggcgtcgac aacgtcgtgg tggtggagac
     1261 ggccgacgcc gtgctggtgg ccgaccgcca gcgcagccag gacgtcaaga agctggtgag
     1321 ccgcctcgac aaggaccgcc gcaccgagca cgcgatgcat cgcaaggtgc accggccgtg
     1381 gggctggtac gacagcgtgg acgcgggccc gcgcttccag gtcaagcgca tcatggtcaa
     1441 tccgggagcg acgctgagcc tgcagatgca ccaccaccgc gccgagcact ggatcgtcgt
     1501 gcagggcacg gcggaggtca cctgcggcga ccgcaagatg atcctgaccg agaaccagag
     1561 cacctacatc ccgctgggcg agacgcaccg cctcgccaac ccgggcaagc tgccgctgga
     1621 gatcatcgag gtgcagtccg gctcctacct cggcgaggac gacatcgtcc gcttcgagga
     1681 cacgtatggc cgcacgagct gaaatggcgc gaaccttgca tgccgtccag gctcagcgcc
     1741 cgcagggcgc cgcatagcct gtcatgttcg aagagcgcag cgtccgcaag cagttcctga
     1801 gtgccccgcc gtcggtgggc agcaccatcg cctccctgct cgagccgctg gtggccgcgg
     1861 gcatgctcgc gctgttccac gagttgtggg gctaccgcat ggacggcgtg gcgatggcgc
     1921 tggccatcct gctgctggtg ctgatgttcc ccggcgtgaa ccgcttcggc cgcaccggcg
     1981 tgggcgtggg catcgacatc atcctgtcgt gggtctgggt gctgagcgtg ctcgcgctgc
     2041 tgggctacgc caccaacacg ctggcctcgt tcgacaccga catgctgatc gcctggggca
     2101 tcgccacgcc gctggtgcag tgggcgctgg tggccatcgg caccgccatc cagcgccacc
     2161 tcgcgtcgct gcccgacgcg cgccggcccg ccgtcatcat cggcgccggc cgcatgggca
     2221 tgcgcgtggc gcagatgctc aagctgcgcc aggccttcgg ccacgacctg ctgggcgtgt
     2281 tcgacgaccg ttcggccgac cgcgtcaacc tgcccgagga ggccgtcctc ctgggcacgc
     2341 tgcaacaact gcccgaatac atcgaggcgc acggcgtgaa ggacgtgttc atcacgttgc
     2401 cgctcacttc gcagccacgc atacagtcct tgctggaatc tttgcagaac accacggcat
     2461 cgatccactt cgttcccgac atcttcggcg tcagcgtcat ccagggccgc ctggaggaca
     2521 tgggcggcgt gccggtcgtg ggcctgatgg tggcgccgtt caccggcatc aacggcttca
     2581 tcaagcgcgt cagcgacatc gtgctgtcga cgctgatcct gatcatgatc tcgccgatcc
     2641 tgctggtgct ggcgatcggc gtgaagatga gctcgccggg cccgatcatc ttccgccagc
     2701 gccgcaccgg cctggacggc gagatcatcg aggtctacaa gttccgctcg atgcgcacga
     2761 ccgaggacgg cgccgtcgtc aagcaggcca cgcgcgacga tccgcgcatc acgccgttcg
     2821 gtgccgtcat ccgcaagacc tcgctcgacg aactgccgca gttcgtcaac gtgctgcagg
     2881 ggcgcatgag catcgtcggc ccgaggccgc acgcggtggc gcacaacgag cagtaccgca
     2941 agctggtcaa ggcctacatg gcgcgccaca aggtcaagcc gggcatcacc ggctgggcgc
     3001 aggtcaacgg cctgcgcggc gagaccgaca cgctcgacaa gatggccgcg cgcgtcgagt
     3061 acgacctgga ataccttcgc aactggacgc tcgggctcga cctgctcatc atcgcgcgca
     3121 cggtcaagct cgtgttcctc gaccgcagcg cgtactgagc cgcgtggccg tcgccgcccg
     3181 cgggcatgcc gttggcgcta ccattttcgg cagcccgggc gcgcgcccgg cgcggcccct
     3241 cgcacgaata ttgccgcgtc gcgcggccgc agtcttcttc tcatccatga cgatcctcgc
     3301 cgacgccgaa ccctccgccc tggcccccgt ggccgcgccg gccgcctccg ctgcagcggc
     3361 ggtcgatgtc gccgtggccg ccacgttcac ggccgagggc ctggaagcgc cgctggcctt
     3421 catgctggac gccgcggggc tggccggccg catcgcgttc gcgccatacc accagctgtt
     3481 ccaggaactg ctgacgcccg gcagcgcgct tgcgcgcaat gccgcgggcg gcggcatcgg
     3541 cgtcgtcgcg ctgcggctgg aggacttcgt gcgtgacgcc gaggccggcg ccgacctggc
     3601 cgcgctcgtc agccgcgtcg cgggcgagct ggccgacgcg ttccgccagt tcaggcagcg
     3661 cgcctcggcg ccgctgctgg tgttcgtgct gcgcgcctcg ccggcgctcg atcgcgggct
     3721 cgcggccgtc gtcgatgcgc agtcggcgtt gctgtcggcc gcgctcgagg cgctgcccga
     3781 caccgccgtc ttcggcgacg cggcggtcga cgcgctgtgc cccgccacgc gccacgaccc
     3841 ggtggccgac aagctggccc acatcccgtt caccgacgac tggttcgccg cgctggccat
     3901 cgtcgtcgcg cgccaggtgc acatgcgcaa ggtcgccgcg cacaaggtgc tggtgctcga
     3961 ttgcgacaac acgatctggc gcggcgtggt cggcgaggac ggcgtcgccg gcatcgcgct
     4021 cgatccgggc ttcctggcgg tgcaggacta cgcggtcgcg gcgcagtcgc gcggcgtgct
     4081 cgtgtgcctg gccagcaaga acgccgaggc cgacgtgatg cgcgtgttcg acgagcgccc
     4141 cgacatgcgg ctggcgcgtg gccacgtcgt cgcgcaccgc atcaactggg agctgaagtc
     4201 ggccaacctg cgcgccctgg cccgcgagct caacctggga ctggacgcgt tcgtgttcct
     4261 cgatgacaac ccggtcgagt gcgggctcat gcaggagatg ctgcccgagg tcgtcacgct
     4321 gcagctgccg tcgcccgagg ccgcggagtc gctgctggcc aacctgtggg cgttcgacaa
     4381 ggtgtcggtg acgcaggagg accagcgccg cacggagatg taccggcaga acgccgagcg
     4441 cgagcagctc gaggcgtccg ccacggacat cggcgcgttc ctcgcgtcgc tggaactgaa
     4501 gatcgacgtg tccgcgcctg ccgagggcga ctggccgcgg ctgtcgcagc tgacccagcg
     4561 caccaaccag ttcaacttca cgacgcgccg ccgcggcgag ccggagatgc gcgccctcgg
     4621 cggaagcacc accgtgcagg ccgtgcgcgt cagcgaccgc ttcggcgact acgggctcgt
     4681 gggcctggtc atcgccgacg agacggtcga cgccatcgag gtcgacacgc tgctgctgag
     4741 ctgccgcgtg ctcgggcgcg gcgtggagca cgcgatgctg gcctcgctcg gccggctggc
     4801 gcagtcacgc ggcaaggccc tggtgcgcct gcacctggtc cgcacgccca agaacgagcc
     4861 cgcgctcgca ttcgccgacg ccgtggtggg cgcataccgg cgcgaggtcg aggacggcgt
     4921 cgcgtgggac gtgccggccg atgtcgccgc cgccatcgtg caccgcgcgg gcgaggacgc
     4981 gcccgaggtc atcgccgcgc gcaaggccga cgccgccaag gcacccaagg ccgccgcgcc
     5041 gtcgctgcgc ggccgctcgg cccgctacgc gcgcctggcc acggagctca cgaccggccc
     5101 cgccgtgatg caggccctgc gcgcggccac gcgcgcggtg cgcgcccccg cggccgcgag
     5161 cgcgcagccg cgcacggcca ccgagcgcgc gctggtcgtg ctgtgggagg agctgctggg
     5221 cgtctcgggg ctgggcatcg acgacgactt cttcgcgctg ggcggcacgt cgctgctggc
     5281 ggcgcgcatg atcgccgagc tcgcgcaccg ccgcggcgtg gcgctgcccc tgacgacgat
     5341 cgtcgaggcg cccaccctcc gccagttcgc gcagcgcgcc gaccagggcg ccgccgcggc
     5401 gccgaaccgc ctggtgacgc tgcgcgagtc gggcgagcgg cgcctgttcc tcgtgcacga
     5461 cggcgacggc gagacgctgc tgtaccgcaa cctcgccaac cggctgccgg ccaccctgag
     5521 cgtctacggc atccagcccg agcgccgccc gcgcatcccg ctcgcgcacc tgtcgatcga
     5581 ggagatggcg gcgtcgtacg tgaagaccgt gcgcgcggtg cagccggacg ggccgtacct
     5641 gctgggcggc atgtgcgccg gcggcctgat cgcgttcgag atggcgcgcc agctgcaggc
     5701 cgacggcgcg gtcgtcgagc gcgtgctgat gatggattcg gccacgccgc aggccgagcg
     5761 gcgcgacatc gagaacgcgc agcgctcgtc gcgcctgcgt gaggccgtgg ccgcggcgcg
     5821 cgaagggcgc ggcgtggtgg gaggactcgt cgcggcggcg ggtgtcgtcg cgcgcaaggt
     5881 cttcggccgc gtcgcgtggg agctgcgcaa gcggcgcctg cagtccgacg acgaccggcg
     5941 cctggccacg ctgcgccagg tcctggcgag cggcagcgag tggccggcgt ccgagcccag
     6001 cctcgacttc cgcgccatct acaaccacgc cgagcacgcc taccggccgc cgccctcggc
     6061 gatcagcgcc gtcctgctgc gcgccacggc cggcagcggc aacgacatcc cgtaccgcga
     6121 ggtgttcgcc gacccgacgc tcggctgggc cggcgtcgcg cgcgacctgg tcgtcgtcga
     6181 cgtcgagggc ggccacgcga gcatgctgca ggagccgcag gccgcggcgc tggcgcgcgc
     6241 gatcctgccg cacgtggccg gcgcggcgat gcgggcggcc gcatgagccc cgcccggccg
     6301 cccgaagggg ctcgcaccgc agtccgcagg acggaggatg ccttatgagc gcgggcgcaa
     6361 gtctcgcgac gatgacggtg ggcatcgtcg tcgtctccta caagtcggcg gcgctgacgg
     6421 tcgactgcct gcgctcgatc gcggcggaac gcgccggccc cgggccgcgc atccgctgcg
     6481 tcgtcatcga caacgcgtcc ggcgacgcgc ccgccgtggc cgcggcgatc gccgagcacg
     6541 gctggggcga ctgggcccgc gtggtggtgg cgccgcg
//
LOCUS       DATCRO010000868         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_628552, whole genome shotgun sequence.
ACCESSION   DATCRO010000868 DATCRO010000000
VERSION     DATCRO010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628552"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VIN75_23630"
     CDS             complement(<1..216)
                     /locus_tag="VIN75_23630"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY8977225.1"
                     /translation="MKVLITGAAGFIGLHVAERLLARGDEVVGLDNLNAYYEPSLKTA
                     RLARLEGRAGFRFERQDIADGAALEALF"
     gene            complement(233..1513)
                     /locus_tag="VIN75_23635"
     CDS             complement(233..1513)
                     /locus_tag="VIN75_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY8977226.1"
                     /translation="MQTVAVVGLGYVGLPLVVEFGKKMRTIGFDIHAGKVASCRSGVD
                     PSREIPDEEMKLAVKAEYTSDPHALSEADFVLVAVPTPVDEAHIPDFAPLVGASQAIG
                     AHLKPGAIVIYESTVYPGATEEVCIPVLEKVSGKTWKKDFFVGYSPERINPGDREHTL
                     TNVLKVVSGDTPQTLEKVAQLYELIVKPGVHRCSSIKAAEACKVIENTQRDLNIALMN
                     ELAIIFDRVGIDTAEVLEAAGTKWNFLKFKPGLVGGHCIGVDPYYLTHKAETLGYHPQ
                     VILAGRRINDGMGKFIAEQTVKQMIAAGSYVKGARVNVLGLTFKEDCADLRNSKVVDI
                     INELRTYGVEVFVHDPQADAQEARHEYGVELAAWDALPRADAIVAAVAHREYRALTVA
                     EVGARVAPGGVFIDVKAAFPEAALAAAGLRVWRL"
     gene            1584..2001
                     /locus_tag="VIN75_23640"
                     /pseudo
     CDS             1584..2001
                     /locus_tag="VIN75_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          283 a          685 c          696 g          337 t
ORIGIN      
        1 gaacagcgcc tcgagcgcgg cgccgtcggc gatgtcctgg cgctcgaagc gaaagcccgc
       61 gcggccctcg agccgggcga ggcgcgccgt cttcagcgac ggctcgtagt aggcgttgag
      121 gttgtccagg ccgaccacct cgtcgccgcg cgccagcagg cgctcggcga cgtgcaggcc
      181 gatgaagccg gcggctcccg tgatgaggac cttcatcgcg ccgcctcccg gctcacaggc
      241 gccagacgcg caggcccgcg gccgcgagcg ccgcctcggg gaacgccgcc ttcacgtcga
      301 tgaacacgcc gccgggcgcg acgcgcgcgc cgacctcggc caccgtcagc gcccggtact
      361 cgcggtgcgc gacggccgcg acgatcgcgt cggcgcgcgg cagcgcgtcc caggcggcga
      421 gctcgacgcc gtactcgtgg cgcgcttcct gcgcatcggc ctgcgggtcg tgcacgaaga
      481 cctcgacgcc gtaggtgcgc agctcgttga tgatgtccac gaccttgctg ttgcgcaggt
      541 cggcgcagtc ttccttgaag gtgaggccga gcacgttcac gcgcgcgccc ttcacgtagc
      601 tgcccgcggc gatcatctgc ttgaccgtct gctcggcgat gaacttcccc atgccgtcgt
      661 tgatgcggcg ccccgccagg atgacctgcg ggtggtagcc cagcgtctcg gccttgtgcg
      721 tgaggtagta ggggtcgacg ccgatgcagt ggccacccac gaggccgggc ttgaacttga
      781 ggaagttcca cttcgtgccg gcggcttcga gcacttcggc ggtgtcgatg cccacgcggt
      841 cgaagatgat ggccagctcg ttcatcagtg cgatgttcag gtcgcgctgc gtgttctcga
      901 tcaccttgca ggcctcggcc gccttgatcg agctgcaacg gtgaacgccg ggcttcacga
      961 tcagctcgta cagctgcgcc accttctcga gcgtctgcgg cgtatcgccg gacacgacct
     1021 tgagcacgtt ggtcagcgtg tgctcgcgat cgccggggtt gatgcgctcg gggctgtagc
     1081 ccacgaagaa gtccttcttc caggtcttgc cggacacctt ctcgagcacc gggatgcaga
     1141 cctcctcggt ggcgccgggg tacacggtgg attcgtagat cacgatggcg ccgggcttca
     1201 ggtgcgcgcc gatggcctgg ctcgcgccca cgagcggcgc gaagtcggga atgtgcgcct
     1261 cgtcgaccgg cgtgggcacg gcgacgagca cgaaatcggc ctcggacagc gcatgcgggt
     1321 cgctcgtgta ttcggccttc accgcgagct tcatctcctc gtcggggatc tcgcgcgagg
     1381 ggtcgacgcc gctgcggcag gaggcgacct tccctgcgtg gatatcgaag ccgatggtgc
     1441 gcatcttctt gccgaactca acgacgagcg gcaggcccac atagcccagt ccgacgacgg
     1501 cgacggtttg catagttcct cctactttcc tgtttcctgg aatgttgatt tcgagggccg
     1561 cgcccggcta ggccgtgcgc gcgggcgccg atcccgccgg cggcatcggc gagatgcgtg
     1621 aggtggagac tcacctgatc ccgcgcgcca tgatggcgct gcaggggcac gtgcccgact
     1681 ttgccgtgtt cggcgacgat tacgatacgc ccgacggcac ggcgatccgc gactacatcc
     1741 acgtgcagga cctcgccgaa ggccacgtgg cggcggtgcg caagctgctg gatgccggtg
     1801 ggtgcttcac cgtgaacctc ggcaccggcc gcggctacag cgtgctcgag ctcgtgcgtg
     1861 cctacgaggc cgccagcggg cgcagcgtgc cgcacgaggt ggtggcgcgc cggcccggcg
     1921 acatcaacag ctgctacgcc gaccccgcac tcgcgcgttc gctgctgggc tgggaagcgc
     1981 gccgcggcct cgacgacatg t
//
LOCUS       DATCRO010000869         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_583878, whole genome shotgun sequence.
ACCESSION   DATCRO010000869 DATCRO010000000
VERSION     DATCRO010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583878"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1819)
                     /locus_tag="VIN75_23645"
     CDS             complement(296..1819)
                     /locus_tag="VIN75_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658378.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY8977227.1"
                     /translation="MGMRVRLEDPVDFDAFRREARRLLADGVPPDHVEWSDGTQGSGG
                     AELFGDAPAGGLGLFSDEPAAPPAPAGAARQPVPASFLALCADVILHRDPGRLALMYR
                     LLWRLAHEPSLRHDPLDADMLRAGLMAKAVRRDIHKMRAFVRFTRVEGEQGERHVAWF
                     EPDHRIVEANAPFFSRRFAQMQWAILTPERCVAWDGQALTFRDGADRRERPPPDAGEQ
                     LWLTYYENIFNPARLKLAMMRREMPVRYWHNLPEAALIQPLADAAQARSQSMIDAPAT
                     PARRIRAPLPLHLLPADRAAPASLDELRAVTHACRDCPIGALATQAVCGEGPVGAQRM
                     LVGEQPGDQEDLAGRPFVGPAGQLLDRAMAQLGRPRDTVYLANAVKHFKYELRGKRRI
                     HKTAGQREAEACAHWLDSEIALVQPRALVALGATAARALLGRPVAVQAERGAWLHERA
                     DGRPVFVTLHPSALLRLPDAERAAAYDRWLADLYGAFESPLGDSGRIQPAAARVLVP"
     gene            complement(1839..>2602)
                     /locus_tag="VIN75_23650"
     CDS             complement(1839..>2602)
                     /locus_tag="VIN75_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEY8977228.1"
                     /translation="VELPTAEGLRALAPEKDESAIRRSMARLRLRIDDTREEARRVVP
                     IRALPGAAPAAAKPPPFAPAGQSTQMIVGADATDDRHILASSATLYGAYKLKRVYYSA
                     FSPIPDAARALPLAAPPLMREHRLYQADWLMRFYGFEHQEIVASPDGLLRLDVDPKLA
                     WALAHAERFPVDLNHAPREMLLRVPGLGVKAVERLLQARRVRRVRADDLRRLHVPARK
                     VLPFVVADGHRPAANAMAMSASAAAAPVPAQAALF"
BASE COUNT          346 a          930 c          979 g          347 t
ORIGIN      
        1 ccaccagcca gtgcgcggcc tgcaggcgca gcacggccgc gcgcgtgagc gggccgaagt
       61 cgccgtcggt ggcgagcgcg gggatgggct tgagtttgcg gttcagggct tcctgcagtc
      121 ggcggacgtc gatgccgcgc caaccctgtt tcagcgtgtt catccagata ctttttctcg
      181 aacgctcgtt cgtatcgcgg acccgggaat ccgagtgccg atggtcgaaa ccaggcctgc
      241 gggcgcacgg ccgccagact ggcgcgctcg gcggcgccgg accatggcgc ttgctttacg
      301 gaaccagtac gcgcgccgcg gccggctgga tgcggccgct gtcgccgagg ggactttcga
      361 atgctccgta caggtcggcc agccagcggt cgtaggcggc ggcgcgttcg gcgtcgggca
      421 gccgcagcag cgcggacgga tgcagcgtga cgaacaccgg ccggccgtcg gcgcgctcgt
      481 gcagccaggc gccccgctcg gcctgcaccg ccaccgggcg gccgagcaag gcgcgcgcgg
      541 cggtggcgcc cagggccacc agcgccctgg gctgcacgag tgcgatctcg ctgtccagcc
      601 agtgggcgca agcctcggcc tcgcgctggc cggcggtctt gtggatgcgg cgcttgccgc
      661 gcagctcgta cttgaaatgc ttgacggcat tggccaggta caccgtgtcg cgcggccggc
      721 cgagctgcgc catcgcgcgg tcgagcagct ggccggccgg cccgacgaag gggcggccgg
      781 ccaggtcctc ctggtcgccg ggctgttcgc cgaccagcat gcgctgcgcg cccacgggcc
      841 cttcgccgca cacggcctgg gtggcgagcg cgccgatggg gcagtcgcgg cacgcatgcg
      901 tgaccgcgcg aagttcatcg aggctggccg gcgccgcacg atcggccggc agcaggtgca
      961 gcggcagagg ggcgcggatg cggcgcgcgg gcgtggccgg cgcgtcgatc atcgactggc
     1021 tgcgcgcctg cgccgcgtcg gccagcggct ggatcaacgc ggcctcgggc aggttgtgcc
     1081 agtagcggac cggcatctcg cggcgcatca tcgcgagctt cagccgcgcg gggttgaaga
     1141 tgttctcgta ataagtgagc cagagttgct cgcccgcatc cggcggcggc ctctcgcgcc
     1201 ggtcggcgcc gtcgcggaaa gtcagcgcct gcccgtccca cgccacgcag cgctccggcg
     1261 tgaggatggc ccattgcatc tgcgcgaagc ggcgcgagaa gaacggcgca ttggcctcca
     1321 cgatgcggtg gtcgggctcg aaccacgcca cgtggcgctc gccctgctcg ccttccacgc
     1381 gggtgaagcg cacgaaggcg cgcatcttgt ggatgtcgcg gcgcacggcc ttggccatca
     1441 gcccggcacg cagcatgtcg gcgtcgagcg ggtcgtgccg cagcgaaggt tcgtgcgcca
     1501 gccgccagag cagccggtac atgagcgcga ggcggcccgg gtcgcgatgc aggatcacgt
     1561 cggcacacag cgcgaggaac gacgcgggca ccggctgtcg cgcggcgccg gccggtgcag
     1621 gcggcgcggc aggctcatcc gaaaacagcc cgagaccgcc cgcgggcgcg tccccgaaaa
     1681 gctccgcgcc gccgctgccc tgcgtgccgt cgctccactc cacatggtcg ggcggcacgc
     1741 cgtcggccag cagccggcgc gcctcgcggc ggaaggcgtc gaaatcgacc gggtcttcga
     1801 gccgcacgcg catacccatg ccgatccttc cctcggcctc agaacagcgc cgcctgcgcc
     1861 gggacgggag cggccgcggc ggacgccgac attgccatcg cattcgccgc cggccggtgc
     1921 ccgtcggcca ccacgaaggg cagcaccttg cgcgccggca catgcaggcg ccgcaggtcg
     1981 tctgccctca cccttcgcac gcgacgcgcc tgcagcagcc gttcgaccgc cttcaccccg
     2041 aggccgggca cgcgcagcag catctctcgc ggggcgtggt tgaggtcgac cgggaagcgc
     2101 tccgcatgcg ccagcgccca ggccagcttg ggatcgacgt ccagccgcag caggccgtcg
     2161 ggcgaggcca cgatctcctg gtgctcgaag ccgtagaagc gcatgagcca gtcggcctgg
     2221 tacagccggt gctcgcgcat cagcggcggc gcggccagcg gcagcgcgcg ggccgcgtcg
     2281 gggatgggac tgaaggccga gtagtacacc cgcttgagct tgtaggcgcc gtagagcgtg
     2341 gcgctggagg ccaggatgtg gcggtcgtcg gtggcgtcgg cgcccacgat catctgcgtg
     2401 ctctggcccg cgggcgcgaa gggcggcggc ttggcggcag ccggcgcggc gcccggcagc
     2461 gcgcggatcg gcaccacccg gcgcgcctcc tcgcgggtgt cgtcgatgcg aagccgcagc
     2521 cgcgccatcg agcggcggat ggcgctctcg tccttctcgg gcgccagcgc tcgcaggcct
     2581 tcggcggtgg gcagctcgac gt
//
LOCUS       DATCRO010000870         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_21786, whole genome shotgun sequence.
ACCESSION   DATCRO010000870 DATCRO010000000
VERSION     DATCRO010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21786"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIN75_23655"
     CDS             <1..195
                     /locus_tag="VIN75_23655"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8977229.1"
                     /translation="DGDEVDIITAMDGAPIASFPELRRALERHRPGDTCRLTVWRAGR
                     TRSVSVILDRANGPAGAARR"
     gene            complement(207..1172)
                     /locus_tag="VIN75_23660"
     CDS             complement(207..1172)
                     /locus_tag="VIN75_23660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8977230.1"
                     /translation="MPQRFRLLRLAAAALACVTCVLAQAQASAPAGAASAPAARTTAD
                     IIKDAPASAWRRPDPNDVLAMTLPSGIVWIELAPRFAPRHADNIRTLVAQHYFDGLSV
                     LRVQDNFVAQWGDRDADDEDATKGRSLGAARRTLEPEFAISDKGLKITRLKDRDVWAP
                     VTGFVDGFPVAADPKTHQAWIAHCYGTIGAGRGNTVDSGNGSELYAVIGQSPRGLDRN
                     IAPVARVLQGMELLSALPRGSGPMGFYDKPEQDTRILKVERLADMPEATRPDVRVFRT
                     DTPQWAEMIESRRNRQDAWYVHKAGAIDLCNISVPVRIEARAATD"
     gene            complement(1193..2893)
                     /locus_tag="VIN75_23665"
     CDS             complement(1193..2893)
                     /locus_tag="VIN75_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY8977231.1"
                     /translation="MTDADARFARLADEEWAWRLGQFPQLATSVGVTAHDDRLERVDA
                     ASRAARLARWRATREALAAIPVDAMSPAARIDRAVYENQLHGLIAAIESHADRMPLNS
                     DSSFHGPLVDLCDAQPLGDAGEYRRFIARLADIPRFFDEHIELMREGLATGYTVPQAV
                     LDGRDQPLAAIASLSDAAATPFWKPFTAFLPGVPTAERAGLQDEARRVIEGQVLPAYR
                     ALLAFLRDTYVPNARRTTAACDLPDGDAFYAAQIRHYTTLDLTADEIHERGLREVARI
                     LAEMHEVRRAAGFDGDLPDFLDFLRADPQFYARTAQELLAHASWISKRIDGLLPKFFG
                     VLPRKPYGVAPVPDEIAPYYTTGRYVPAPRPTGCAGTYWVNTHDLPSRALYTLPALTL
                     HEAVPGHHLQIALAAEVQDQPAWRRHSFVDAYGEGWALYAEHLGAEMGVYLTPYEQFG
                     RLTYEMWRAARLVVDTGLHARRWSREQARAYLLEHTALSAHEVATEVDRYVGWPGQAL
                     SYKIGEITIRELRARAEQSLGERFDLRAFHDAVLALGSVPLGLLEERIAAWIAVRLAS
                     "
     gene            complement(2908..>3351)
                     /locus_tag="VIN75_23670"
     CDS             complement(2908..>3351)
                     /locus_tag="VIN75_23670"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8977232.1"
                     /translation="SSADIRRIAAIRHRLGERLAILVGVDDIIVEGIDAGATGWVAGL
                     VNAMPVESVRLFDLAMRARSPDVLPATQAQVRAEADALYAWFHALLTLDTVPKFVQLI
                     KLVQQQVGHGHERVRAPRLVLEGAEREQALALIAAKLAVRPRLRA"
BASE COUNT          450 a         1191 c         1252 g          458 t
ORIGIN      
        1 gacggcgacg aggtggacat catcacggcc atggacggcg cgcccatcgc gtcgtttccg
       61 gagctgcgcc gcgcgctcga gcggcaccgg cccggcgaca cctgcaggct cacggtctgg
      121 cgcgccggca ggacgcgcag cgtctccgtc atcctcgacc gtgccaacgg gccggccggc
      181 gccgcccggc gctgacgcgt gcggcctcag tcagtcgcgg ccctggcctc gatgcgcacc
      241 ggcacgctga tgttgcacag gtcgatcgcg ccggccttgt gcacgtacca cgcgtcctgg
      301 cggttgcggc gcgactcgat catctccgcc cactgcgggg tgtcggtgcg gaagacgcgc
      361 acgtccggcc gcgtggcctc gggcatgtcg gccaggcgct cgaccttcag gatgcgagtg
      421 tcctgctcgg gcttgtcgta gaagcccatc ggaccgctgc cgcgcggcag cgccgacagc
      481 agttccatgc cctgcagcac gcgcgccacc ggggcgatgt tgcggtccag gccgcgcggc
      541 gactggccga tcacggcgta cagctccgag ccgttgcccg agtcgaccgt gttgccgcgg
      601 cccgcgccga tcgtgccgta gcagtgcgcg atccaggcct ggtgcgtctt gggatcggcc
      661 gccacgggaa agccgtcgac gaagccggtg accggggccc agacgtcgcg atccttcaac
      721 ctcgtgatct tcaggccctt gtcgctgatg gcgaactccg gctcgagggt gcgcctggcg
      781 gcgccgagcg aacggccctt ggtggcatcc tcgtcgtccg cgtcgcggtc gccccactgc
      841 gcgacgaagt tgtcctgcac gcgcagcacg gacaggccgt cgaagtagtg ctgggcgacc
      901 agcgtccgga tgttgtcggc gtgcctgggc gcaaagcgcg gcgcgagctc gatccacacg
      961 atgcccgagg gcagcgtcat cgcgagcacg tcgttcgggt cgggccggcg ccaggcgctg
     1021 gccggcgcgt ccttgatgat gtcggccgtg gtgcgcgcag ccggcgcgga cgcggcacct
     1081 gcgggcgcgg aggcctgggc ttgcgcgagc acgcacgtga cgcacgcgag cgcggcggcg
     1141 gccaggcgca gaagtcggaa acgttggggc acggggttcc tcggacgatg ggtcaggaag
     1201 cgaggcgcac cgcgatccac gcggcgatgc gctcctcgag aaggcccaag ggtaccgaac
     1261 ccagcgccag cacggcgtcg tggaatgcac gcaggtcgaa gcgctcgccc agagactgct
     1321 cagcccgcgc gcgcagctcg cggatcgtga tctcgccgat cttgtacgac agcgcctgcc
     1381 ccggccagcc gacatagcgg tcgacctccg tggccacctc gtgcgcactg agcgcggtgt
     1441 gctccagcag gtaggcgcgc gcctgctccc ggctccagcg cctggcgtgc aggccggtgt
     1501 cgacgaccag ccgcgccgcg cgccacatct cgtacgtgag ccgcccgaac tgctcgtacg
     1561 gggtgaggta cacgcccatc tcggcgccca ggtgctcggc gtaaagcgcc cagccctcgc
     1621 cgtacgcgtc gacgaagctg tggcgccgcc acgcgggctg gtcctggacc tcggccgcga
     1681 gcgcgatctg caggtggtgg ccgggcaccg cctcgtgcag tgtcagcgcg ggcagcgtgt
     1741 agagcgcgcg cgacggcagg tcgtgcgtgt tgacccaata ggtgccggcg cagcccgtgg
     1801 gccgcggcgc gggcacgtag cggcccgtgg tgtagtacgg cgcgatctcg tcaggcaccg
     1861 gcgccacgcc gtagggtttg cgcggcagca cgccgaagaa cttcggcagc agaccgtcga
     1921 tgcgcttgga gatccaggag gcgtgcgcca ggagctcctg cgccgtgcgg gcgtagaact
     1981 gcgggtcggc acgcaggaaa tcgaggaagt ccggcaggtc gccgtcgaag cccgcagcgc
     2041 gccgcacctc gtgcatctcg gccaggatgc gcgccacctc gcgcaggccg cgctcgtgga
     2101 tctcgtcggc cgtcaggtcc agcgtcgtgt agtggcggat ctgcgccgcg tagaacgcgt
     2161 cgccgtccgg caggtcgcag gcggcggtcg tgcggcgcgc gttcggcacg taggtgtcgc
     2221 gcaggaacgc gaggagcgcg cggtaggccg ggagcacctg gccctcgatg acgcggcgcg
     2281 cctcgtcctg cagcccggcg cgctccgcgg tgggcacgcc gggcaggaac gccgtgaacg
     2341 gcttccagaa cggcgtggcg gcggcgtccg acaggctcgc gatcgcggcc agcggctggt
     2401 cgcggccgtc cagcaccgcc tgcggcaccg tgtagccggt ggccaggccc tcgcgcatca
     2461 gctcgatgtg ctcgtcgaag aagcgcggga tgtcggccag ccgcgcgatg aagcggcggt
     2521 actcgccggc gtcgcccagc ggctgcgcgt cgcacaggtc gaccagcggc ccgtggaagc
     2581 tcgagtcgct gttgagcggc atccggtcgg catgcgactc gatggccgcg atgagcccgt
     2641 gcagctggtt ctcgtagacc gcgcggtcga tgcgcgccgc cggcgacatg gcgtcgacgg
     2701 ggatcgccgc cagcgcctcg cgcgtggccc gccagcgcgc gaggcgcgcg gcgcggctgg
     2761 ccgcgtcgac gcgctccagc cggtcgtcgt gcgccgtgac gccgacgctg gtggccagct
     2821 gcgggaactg gcccaggcgc caggcccatt cctcgtcggc gagccgggcg aagcgggcgt
     2881 cggcgtcggt catcgcggcg tccatgctca ggcgcgcagg cgcggacgca cggccagctt
     2941 cgcggcgatc agcgccagcg cctgttcgcg ctcggcgccc tcgagcacca ggcgcggcgc
     3001 gcgcacgcgc tcgtggccgt ggcccacctg ctgctgcacc agcttgatca gctgcacgaa
     3061 cttcgggacg gtgtcgagcg tcagcaacgc gtggaaccag gcgtagagcg cgtcggcctc
     3121 ggcgcgcacc tgggcctgcg tcgcgggcag cacgtcgggc gatctggcac gcatcgcgag
     3181 gtcgaacagg cgcaccgatt cgaccggcat cgcgttgacc agccccgcca cccagcccgt
     3241 cgcgccggca tcgatgccct cgacgatgat gtcgtccacg ccaaccagga tcgccaggcg
     3301 ctcgcccagg cgatggcgga tcgcggcgat gcggcggatg tcggcgctcg a
//
LOCUS       DATCRO010000871         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_921497, whole genome shotgun sequence.
ACCESSION   DATCRO010000871 DATCRO010000000
VERSION     DATCRO010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921497"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VIN75_23675"
     CDS             <1..403
                     /locus_tag="VIN75_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8977233.1"
                     /translation="GGDTHANGSAPTAKSSKPRRGTAAPLHEIVPGQHVRANSPDVLI
                     RLARSGAGVVAVADFFAEPYLARGELQRILPAWCLPDAQCWAVFPGRRLMPAKTRAFV
                     DMLAKAMSTCSEAIDEAAAKLNAPPGRFRVD"
     gene            complement(422..850)
                     /locus_tag="VIN75_23680"
     CDS             complement(422..850)
                     /locus_tag="VIN75_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY8977234.1"
                     /translation="MCEIFISADPASYESRTRSVRLHGVVTSIRLEHLYWEVLEEIAR
                     RDGMSVTHLIEKLYDELVAARGGVGNFTSFLRVSALRYEALVARGRIPADVGVPIRSL
                     DAKTVLRELPRGWTTPSEAASAGDAPPARALQRALTRLPH"
     gene            complement(984..1562)
                     /locus_tag="VIN75_23685"
     CDS             complement(984..1562)
                     /locus_tag="VIN75_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY8977235.1"
                     /translation="MGKKILMICGDFGEDYETMVPFQALQAVGHTVHAIAPGKKAGDY
                     VTMAIHDFDGAQTYSEKPGHRFTLNASFADTRAESYDALVVPGGRAPEYLRMNSQVVA
                     MTKHFLSAGKPVAAICHGAQLLAATGLIQGRKVSAYPACQVEVELAGAEYIGVAVDQA
                     VTDGNLVTAPAWPAHPAWIAQFLAVLGTRISV"
     gene            1656..2402
                     /locus_tag="VIN75_23690"
     CDS             1656..2402
                     /locus_tag="VIN75_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977236.1"
                     /translation="MTSPPALTELHRRRLRAIWRSAGWPSQDLVEVELLAAGWVERMR
                     DGHGRETLRVTDAGIQVLAATLARNRAARDAHEALIGRVAVAMQRAGRIAWRGLSLRV
                     RTGDDAAGTGQWTVARPDLFSVRNTTVEDYLEPVVHEIKVRRADLLGDLRRPAKGEAY
                     RQMARECWYVLAEGIGDAGDVPEDYGVMVELGGVLDVLRPAPRRALRLPFATWMALAR
                     ATPEPHEESGQQALGCPDDDGRHDAGDAPA"
     gene            2553..>3262
                     /locus_tag="VIN75_23695"
     CDS             2553..>3262
                     /locus_tag="VIN75_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEY8977237.1"
                     /translation="MIRLSPTSARSLKSSALVIALAAAVAGFGSAAHAVDGPPPGNVA
                     WQDASSDADIDKAFATAKAQNKPVLLYWGAVWCPPCNQLKATLFNRQDFAEQSRSFVA
                     VHLDGDAPGAQRLGTKFKVVGYPTLILFSPDRKELTRLPGEVDAAQVMQLLQLGMASG
                     RPIAQVLADAREGKPLKGSEWRLLAFYSWDTDEGAQAVPAAERGPLLRKLAANCPASE
                     AAACTRLVLKSIGDGDEK"
BASE COUNT          447 a         1185 c         1174 g          456 t
ORIGIN      
        1 gggcggagac acccacgcca acgggtccgc gcccacggcg aagtcctcga agccccgccg
       61 cggcaccgcg gcgccgctgc acgagatcgt ccccggccag cacgtgcgcg cgaactcgcc
      121 cgacgtgctg atccgcctgg cgcgcagcgg cgcgggcgtg gtggccgtcg ccgacttctt
      181 cgccgagccc tatctcgcgc gtggcgagct gcagcgcatc ctgccagcct ggtgcctgcc
      241 ggacgcgcaa tgctgggccg tgttccccgg ccgccgcctg atgcccgcca agacgcgcgc
      301 gttcgtcgac atgctcgcca aggcgatgag cacgtgctcg gaagcgatcg acgaagccgc
      361 ggcgaagctc aacgctccgc cgggacgatt tcgcgtggac tgagccgcgc ccgcggccgt
      421 ttcagtgcgg caggcgcgtg agtgcgcgct gcagggcgcg cgcgggtggg gcgtcgccag
      481 cgctggccgc ctcgctgggc gtcgtccagc cgcgcggcag ttcgcgcagc accgtcttgg
      541 cgtcgagcga gcggatcggc acgcccacgt ccgccgggat gcggccgcgc gccaccagcg
      601 cctcgtagcg cagcgcgctg acccgcagga acgacgtgaa gttgccgacc ccgccgcggg
      661 cggccaccag ctcgtcgtac agcttctcga tcaggtgcgt gacgctcatg ccgtcgcggc
      721 gcgcgatctc ctcgagcacc tcccagtaca agtgctccag ccggatgctc gtgaccacgc
      781 catgcaggcg gaccgagcgg gtgcggcttt catagctcgc aggatcggcg ctgatgaaga
      841 tttcacacat gcagatgtct ccttggcctg gccgcgtcgc ttctggcgtg cggctctgcg
      901 gagaggtggc gcaggatgcg gccacctctc gcggaccaat gtacgcccgg gcgggcaaag
      961 tcgccaagcg cggaacggcg gatttacacg ctgattcgcg tgcccagcac cgccaggaac
     1021 tgcgcgatcc aggccggatg cgcgggccag gcgggtgccg tcaccaggtt gccgtcggtc
     1081 acggcctggt cgaccgcgac accgatgtac tcggcgccgg cgagctccac ctcgacctgg
     1141 caggccgggt aggcggacac cttgcggccc tggatcaggc cggtggcggc gagcagctgc
     1201 gcgccgtggc agatcgcggc gacgggcttg ccggccgaca ggaagtgttt cgtcatcgcg
     1261 accacctgcg agttcatgcg caggtattcg ggcgcgcgcc cgccgggcac caccagcgcg
     1321 tcgtaggact cggcgcgggt gtcggcaaag ctcgcgttga gcgtgaaccg gtggccgggc
     1381 ttctcgctgt aggtctgcgc gccgtcgaag tcgtggatgg ccatcgtcac gtagtcgccg
     1441 gccttcttgc ccggggcgat cgcatgcacc gtgtggccga ccgcctgcag cgcctggaac
     1501 ggcaccatcg tctcgtagtc ctcgccgaaa tcgccgcaga tcatcaggat cttcttgccc
     1561 atgctcgtct ccagaagggt ggttgaagga acgcctcgat tgttcgaagc tcgcgccatg
     1621 agcgggtagc aacccgatac tttcgtgtgc tcgacatgac ctcgccgccc gccttgaccg
     1681 aactgcaccg ccgccgcctg cgcgcgatct ggcgctcggc cggctggccc agccaggacc
     1741 tggtcgaggt ggagctgctc gccgcgggct gggtcgagcg gatgcgcgac ggccatggcc
     1801 gcgagacgct gcgcgtcacc gatgccggca tccaggtgct ggccgcgacg ctcgcgcgca
     1861 atcgcgccgc gcgcgacgcg cacgaggcgc tgatcggccg cgtggccgtc gccatgcagc
     1921 gcgcgggccg catcgcgtgg cgcgggctgt cgctgcgcgt gcgcaccggc gacgacgcgg
     1981 ccggcaccgg ccagtggacg gtcgcgcggc ccgacctgtt ctcggtgcgc aacacgaccg
     2041 tggaggacta cctcgagccc gtcgtccatg agatcaaggt ccgccgcgcc gacctgctcg
     2101 gcgacctgcg ccggcccgcc aagggcgagg cctaccggca gatggcgcgc gagtgctggt
     2161 acgtgctggc cgagggcatc ggcgacgccg gcgacgtgcc ggaggactat ggcgtgatgg
     2221 tcgagctcgg tggcgtgctg gacgtgctgc gcccggcccc gcgccgcgcg ctgcgcctgc
     2281 cgttcgcgac ctggatggcg ctggcgcgcg cgacgcccga gccgcacgag gaatcggggc
     2341 agcaggcgct cggttgcccg gacgacgacg gccgccacga cgccggcgac gcgcccgcct
     2401 gagcccgcgc acccgcgggc gtgacgcatg cgcatgcgcg cccgcgctgg ctgacaatac
     2461 gcgtttcgcg cgcggcccgc ccgcgccgct cgacgcccac ccggcgccgg gcgggttcgg
     2521 ttgccgccat ccacgtcctg gagaccgttt cgatgatccg cttgtcgccc acctccgccc
     2581 gttccctgaa gtcgtccgcc ctcgtcatcg cgctcgccgc ggcggtggcc ggcttcggct
     2641 cggccgcgca tgccgtcgac ggcccgccgc cgggcaacgt ggcctggcag gacgccagca
     2701 gcgacgccga catcgacaag gccttcgcca ccgccaaggc gcagaacaag ccggtgctgc
     2761 tgtactgggg cgccgtgtgg tgcccgccgt gcaaccagct caaggccacg ctgttcaacc
     2821 gccaggactt cgccgagcag tccaggagct tcgtggccgt gcacctggac ggcgacgcac
     2881 cgggcgcgca gaggctgggc acgaagttca aggtggtcgg ctacccgacg ctgatcctgt
     2941 tcagccccga ccgcaaggag ctcacgcgcc tgcctggcga ggtcgacgcc gcgcaggtga
     3001 tgcagctgct gcagctgggc atggccagcg ggcgcccgat cgcgcaggtg ctggccgacg
     3061 cgcgcgaggg caagccgctc aagggcagcg aatggcgcct gctcgcgttc tactcgtggg
     3121 acaccgacga gggcgcgcag gccgtgccgg ccgccgagcg cggccccctg ctgcgcaagc
     3181 tcgcggccaa ctgcccggcg agcgaggcag cggcgtgcac gcgtctcgtg ctgaagtcca
     3241 tcggcgacgg cgacgagaag aa
//
LOCUS       DATCRO010000872         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_190001, whole genome shotgun sequence.
ACCESSION   DATCRO010000872 DATCRO010000000
VERSION     DATCRO010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190001"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..>3028
                     /locus_tag="VIN75_23700"
     CDS             123..>3028
                     /locus_tag="VIN75_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977238.1"
                     /translation="MTTPRVAVIAVHGVADQQPGDTARAVVELLVSSAPDGTCYTSSS
                     GEALALRVPPLAPQAGAVPQVERESQATPSSEDRSVAKSFRQSLRSDFHRHGWAEDTP
                     AAPPPAAPGAAAAQAELGVPAVDKDRGIALTNYLLAKHLRNGATPETYDTACTALERH
                     GVGPDQLRAGVDTKTADVDIYEMYWADLSRLSGSMPRIVSELFTLVFRLSKLGRGTVD
                     EMSGQLRRARVPGTPWARAYAWAWSVLAWLQIALDWLFVNGLALLFAQLALLGVLLVF
                     LGFTSGLAPEITHDAAAREAARTHQTWLHMGIAGALIVLAGLRLLYRRGDGKWLQAAA
                     PVALMGLAIAAIVVGALSGPGLLQWLTAITFLVLLTLANQSMLRIADDRFPLVHAAGS
                     WMWGASALLMVASAIHEMPTLDSEQWREVWVHAALFTTETVLLAIKWWWIVAGPLLVA
                     WFVAGQVARLESGHASRASVATGRLGITVSIGMFLVMTMTIWALGNNVLSQSVDGVAY
                     QPCVFAYAPVPGDALPASTAGPNACMWQGRNAAREAAVAPMPTASTVLYDRYANSTSA
                     FSVLATLLMLLVAYLVVMFTPSVLAELKLIVDARRDAHRQELDRRRNRQGAARPREAG
                     PDRRVPLAAQARRARELGRWLTAGYRRLDLAVAVITAFGVVLSAGITLVFLSTVWDHD
                     KVAAWIAAAGLTEAMGWIGGLQTRASGATRTLFAPLQALVFSAAGIGAALSVLGGTLS
                     RRLPSLRGPLDVAMDVDNHFREFPRTDIPRAHIFSRYVALLEHVRSRGYDRIVIVSHS
                     QGTVISAELLRFLSSRDQAAPRPGDAPVVHGSALPPVSLLTLGCPLRQLYAARFPGLY
                     AWVLARNGAMFGPRAADVGVQRWMNAFCSGDYVGRWLWSHAGDEAALRHPMVDTLDAD
                     PFGRADAYTGFDPMPPVDTVLDAAREAEVCLGLGAHTHYFERDQTTVAWMIDYLV"
BASE COUNT          353 a         1131 c         1099 g          445 t
ORIGIN      
        1 ctgccgggcg gcgcgccggc ctgagcgtcc cctggtcgag ggagcgcgcc ggcttgacgc
       61 tcgcgcgcgg gctcaactaa cgtggcccgg cgccggtgga cggcgcaccg aggagacccg
      121 ccttgaccac tccccgcgtc gccgtcatcg ccgtgcacgg cgtggccgac cagcagcccg
      181 gcgacaccgc gcgcgccgtc gtcgaactgc tggtgtcgtc cgcccccgat ggcacgtgct
      241 acacgtcgtc gtccggcgag gcgctggcgc tgcgcgtgcc gccgctggcg ccgcaggccg
      301 gcgcggtgcc ccaggtcgag cgcgagtcgc aggccacgcc cagcagcgag gatcgctcgg
      361 tggccaagtc gttccggcag tcgctgcgct cggacttcca ccgccacggc tgggccgagg
      421 acacgcccgc cgccccgccc ccggccgcgc cgggcgccgc cgccgcgcag gccgagctcg
      481 gcgtgccggc ggtcgacaag gaccgcggca tcgcgctgac caactacctg ctggccaagc
      541 acctgcgcaa cggcgcgacg ccggagacct acgacaccgc ctgcaccgcg ctggaacgcc
      601 acggcgtcgg ccccgaccag ctgcgcgccg gcgtcgacac gaagaccgcc gacgtcgaca
      661 tctacgagat gtactgggcg gacctgtcgc gcctgtccgg ctcgatgccg cgcatcgtct
      721 cggagctgtt cacgctcgtg ttccggctgt ccaagctcgg gcgcggcacg gtcgacgaga
      781 tgagcggcca gctgcggcgc gcgcgcgtcc cgggcacgcc ctgggcgcgt gcctacgcgt
      841 gggcctggag cgtgctcgcc tggctgcaga tcgcgctcga ctggctgttc gtcaacggcc
      901 tggcgctgct gttcgcccag ctcgcgctgc tgggcgtgct gctggtgttc ctgggcttca
      961 cctcgggcct ggcgcccgag atcacgcacg acgccgcggc gcgcgaggcc gcacgcacgc
     1021 accagacctg gctgcacatg ggcatcgccg gcgcgttgat cgtgctggcc ggcctgcggc
     1081 tgctctatcg ccgcggcgac ggcaagtggc tgcaggccgc ggcgccggtc gcgctgatgg
     1141 ggctggccat cgcggcgatc gtcgtcggcg cgttgtccgg cccgggcctg ctgcagtggt
     1201 tgacggccat cacgttcctc gtgctgctga cgctggccaa ccagtcgatg ctgcgcattg
     1261 ccgacgaccg cttcccgctg gtgcacgccg cgggcagctg gatgtggggc gcctccgcgc
     1321 tgctgatggt ggccagcgcc atccacgaga tgcccacgct cgattcggag cagtggcgcg
     1381 aggtgtgggt gcacgccgcc ctgttcacca ccgagacggt gctgctggcc atcaagtggt
     1441 ggtggatcgt cgcggggccg ctgctggtcg cgtggttcgt cgcgggccag gtcgcgcggc
     1501 tcgagtcggg ccacgcgtcg cgcgcgagcg tggcgacggg gcgcctgggc atcaccgtgt
     1561 cgatcggcat gttcctggtc atgacgatga cgatctgggc gctcggcaac aacgtgctga
     1621 gccagtccgt cgacggcgtg gcctaccagc cctgcgtgtt cgcctatgcc ccggtcccgg
     1681 gcgatgcgct gccggcgtcc accgccggcc ccaacgcctg catgtggcag ggccggaacg
     1741 ccgcgaggga ggcggccgtg gcgccgatgc ccacggccag caccgtgctg tacgaccgct
     1801 acgccaactc cacgagcgcc ttctcggtgc tcgccaccct gctgatgctc ctggtggcct
     1861 acctggtggt gatgttcacc cccagcgtgc tcgcggagct caagctgatc gtcgatgccc
     1921 gccgcgacgc gcaccggcag gaactggatc ggcgccgcaa tcgccagggc gcggcccggc
     1981 cgcgcgaggc cgggcccgac cgccgcgtgc cgctcgcggc gcaggcccgg cgtgcacgcg
     2041 agctggggcg ctggctcacg gccggctacc ggcgcctgga cctggcggtg gccgtcatca
     2101 cggcgttcgg cgtcgtgctg agcgcgggca tcacgctggt cttcctgtcc accgtctggg
     2161 atcacgacaa ggtggcggcg tggatcgccg ccgccggcct gaccgaggcg atgggctgga
     2221 tcggcggcct gcagacgcgc gcctccggcg cgacgcgcac gctgttcgcg ccgctgcagg
     2281 cactggtctt cagcgccgcg ggcatcggtg cggcgctgtc ggtgctgggc ggcacgctgt
     2341 cgcgccgcct accgtcgctg cgcggcccgc tggacgtcgc gatggacgtg gacaaccact
     2401 tccgcgagtt cccgcgcacc gacatcccgc gcgcccacat cttctcgcgc tacgtcgcgc
     2461 tgctggagca cgtgcgctcg cgcggctacg accgcatcgt catcgtgtcg cacagccagg
     2521 gcaccgtcat cagcgcggag ctgctgcgct tcctgtccag ccgggaccag gccgcgccgc
     2581 ggcccggcga cgcgcccgtc gtgcacggca gcgcgctgcc gccggtcagc ctgctcacgc
     2641 tgggctgtcc gctgcgccag ctgtatgcgg cgcgctttcc cgggctgtac gcgtgggtgc
     2701 tggcgcgcaa cggcgcgatg ttcgggccgc gcgcggccga cgtcggcgtg cagcgctgga
     2761 tgaatgcgtt ctgcagcggc gactacgtcg gacgctggct ctggagccac gccggcgacg
     2821 aggcggcctt gcggcacccg atggtcgaca cgctggacgc cgacccgttc ggccgcgccg
     2881 acgcctacac cggcttcgac ccgatgccgc cggtcgacac ggtgctggac gccgcgcgcg
     2941 aggccgaggt ctgcctcgga ctgggcgcgc acacgcatta cttcgaacgc gaccagacca
     3001 ccgtcgcctg gatgatcgac tacctggt
//
LOCUS       DATCRO010000873        14716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_358070, whole genome shotgun sequence.
ACCESSION   DATCRO010000873 DATCRO010000000
VERSION     DATCRO010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358070"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VIN75_23705"
     CDS             complement(<1..218)
                     /locus_tag="VIN75_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977239.1"
                     /translation="MRTHPLVALAVVAATITTVAAVGQSIPVPPQQPLKKAHHADAAL
                     DARPAASTAVPRWLIAPPASAASGAAAAA"
     gene            complement(223..1983)
                     /locus_tag="VIN75_23710"
     CDS             complement(223..1983)
                     /locus_tag="VIN75_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY8977240.1"
                     /translation="MARKLPSVDALLVGFGWTGAIMAQELTQAGIDVLALERGAWADT
                     SREFATTFAQDELRYYWRHDLFQAAAHDTQTIRNDASQVALPMRRLGSYLPGTNVGGA
                     GVHWNGQTWRFLPTDFVARSHNEQRYGAAALPDTMTIQDWGITYDELEPHYDKFEKVC
                     GISGKAGNLRGEIQAGGNPFEGVRQNEYPNPPMAMSYPPTLFAEAARGLGLHPFPQPS
                     ANMSRAYVNPYGVRLEACTYCGFCEKFACGNYSKASAQTTILPALMKKPNFRLRTECE
                     VMKVNLDSSGKRAVSVTYVDASGEEFEQPADMIVLCSYILNNVRLMLLSGIGAPYDPQ
                     TGQGVIGKNYAYQITSSVNLFFDDRILNPFIGAGAHGMLVDEWNGDNFDHSGLGFIGG
                     GYLGVVQTGARPIETHPVPDGTPKWGSAFKKAIAANYLKTMSIATHGAVMSHRGNFLD
                     LDPTYRDVYGRPLLRMTFDFTDNEHRMSDYLTDRATDIARAMKPRELVINKRTGSWDV
                     VPYQTTHNTGGAIMGADPRTSAVNKYLQSWDVSNLFVMGSSAFPQNPGYNPTGTVAAL
                     AYHSAAAITQRYLKAPGALV"
     gene            complement(1985..2731)
                     /locus_tag="VIN75_23715"
     CDS             complement(1985..2731)
                     /locus_tag="VIN75_23715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY8977241.1"
                     /translation="MDEPRLSRRRLLTAGAVIGAVPVATAAAAAALGGREVPDKEGHA
                     NAPVGVENTSGWTWFTDAEAAFVSAATGRLIPADDLGPGAVEAGVPVFIDRQLAGPYG
                     RGERWYMQGPWGPGTSTQGWQTRLTPAGMYRAAIKAIDAVVAREGKAATFAKLAAADQ
                     DAFLKKLESGDAKLDGVDAKEFFKQLLQNTLEGFWSDPVYGGNRDMVGWKLIGFPGAR
                     YDQSEFVGKHGEKYPLPPVGIRGRVEWTRS"
     gene            complement(2939..4333)
                     /gene="fumC"
                     /locus_tag="VIN75_23720"
     CDS             complement(2939..4333)
                     /gene="fumC"
                     /locus_tag="VIN75_23720"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8977242.1"
                     /translation="MSTDQRIEHDAWGDIPVPADHLWGAQTQRSIEHFAISTERMPPE
                     LLQALALVKSAAARVNERAGRLPAAKADAIARAAAEVIEGRWDAEFPLSVWQTGSGTQ
                     SNMNMNEVLANRASQLLGGPTGLKRLVHPNDEVNLGQSSNDVFPTAMHVAAVQGLAQH
                     VIPALQGLRATLDAKARAFDGIVKIGRTHLQDATPLTLGQEISGWVAQLDHAERALHA
                     ALPELRELALGGTAVGTGLNTPASFGADVAAELDRMTGLGFVTAPNKFAALAAHDAIV
                     RAHGALKALAAALTKIANDVRWLASGPRSGLGELQIPENEPGSSIMPGKVNPTQCEAL
                     TMLCCQVIGNDVAVGMAGASGNFELNVYKPVMIHNLLQSVRLLDDGMRGFDRHCAQGI
                     EPHRERIAELLHNSLMLVTALNPHIGYDAAAQIARTAHAKGLKLRDAAIASGKLTGEQ
                     FDAWVRAEDMVRPD"
     gene            4479..5060
                     /gene="phaR"
                     /locus_tag="VIN75_23725"
     CDS             4479..5060
                     /gene="phaR"
                     /locus_tag="VIN75_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY8977243.1"
                     /translation="MVTTRRTTKASADPAAPEGLRILKKYPNRRLYDTLTSSYITLAD
                     VKKMVLEGAAFEVRDAKTGDDLTRSILLQIILEEETGGMPMFNTQMLAQIIRFYGHAM
                     QGVMGSYLEKNLAALADMQARFAEGGKGLYDATALTPDGWAKFMSGQAPMLQNLMGSY
                     LEQSKKMFEQMQEQMGKPGEAMFPIPGFPPAKR"
     gene            5347..5487
                     /locus_tag="VIN75_23730"
     CDS             5347..5487
                     /locus_tag="VIN75_23730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977244.1"
                     /translation="MLERLLSEPAFRQQAQAFARKYAGHTRDSTARTVFEILQGLLPR
                     AA"
     gene            complement(5509..5784)
                     /locus_tag="VIN75_23735"
     CDS             complement(5509..5784)
                     /locus_tag="VIN75_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY8977245.1"
                     /translation="MCRNIRTLFNFDPPATEIEIRDASLQFVRKLSGFTVPSRANEAA
                     FERAVDEVAASARALIASMVTTAPPHNREVEAAKAKARSVARFGPRG"
     gene            complement(5907..6497)
                     /locus_tag="VIN75_23740"
     CDS             complement(5907..6497)
                     /locus_tag="VIN75_23740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977246.1"
                     /translation="MTTLDRTRFEHLYLNANSIAEIALLRALDRAAAPRPEPTLEQRA
                     ANEALVHALNHPDPATFDWSALQGVYGFDGPLDAAPAAAGTTAPPPARLPGFDEMVGF
                     DDDEDLDAPVDIGRYLQRVDAARPQLQPRIDDCFARADIAALESALATLPGDRPPLTA
                     TLTPDAFTRWLELYEAHHDGMEATVHGIRRLEPILG"
     gene            complement(6520..7830)
                     /locus_tag="VIN75_23745"
     CDS             complement(6520..7830)
                     /locus_tag="VIN75_23745"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY8977247.1"
                     /translation="MTVNDHDSLSPREAAYIAHNCYFTLKDWMTGTAVAGVETRANVR
                     QMVLGDGKSAQQKAGGANTTLRGTDVGGARLDRVFQGSTAGLRTGFGYILRFNRGNSR
                     HVIVATRGTRAEHSKADLYTDAHGSLTGFGGYGLVHTGFKNTFDSIRGGLASDDRHVM
                     DADIVHCVGHSLGGAVATLVAAHYAERGKAVKLYTFGCPRVGAFGAQRAMEQALGKLN
                     VYRVSHDLDPVTMVGPYPFGHLNPNPDDVNNMMMTSPTGRLLSTANHDMNEYVLRVSR
                     DRMSWDSVRALGAAVDHDNAVMARWLLRSSDNPNWITRQTAKGLSYLMKALSGLLSAA
                     GLIGVTAFTAVDLFMAVLASNMSRMADLDADICEAMRQAAGWAGVVIKSAAHITAQVV
                     RAILETMMRVLRPIAVQALFLAGTTGDALPLVIAGASSLTGSVL"
     gene            complement(7877..8698)
                     /locus_tag="VIN75_23750"
     CDS             complement(7877..8698)
                     /locus_tag="VIN75_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977248.1"
                     /translation="MILGLFKGKTSSVVDASVGAPLVRQPALPATFTAPATPGYPQVR
                     DLGGDALPPAGKLTPQESDSQPIRAYVAPGSAGHPGVAVVNIYEPRRETQLWQMAPHD
                     DLRFGSRRAAQFSPDQARWVAFDAESVTALPAGRVLIHLTCDKPVPTDGLYVYDLAAD
                     RLALLAEGVTPDWSQGLPFKFVDTLQVTPDAVLVLFHSDKERLGPQRYVNRFDHLMLF
                     SSAHRDGVPVLKLGLDDGNVRQWGLVGKTLWLRTSDDRGAKPREAIWSLDLAKVL"
     gene            9240..10643
                     /gene="rimO"
                     /locus_tag="VIN75_23755"
     CDS             9240..10643
                     /gene="rimO"
                     /locus_tag="VIN75_23755"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY8977249.1"
                     /translation="MESTTYPTTETPAGGKEPTIAFVSLGCPKALTDSELILTQLRAE
                     GYGTSKSYSGADLVVVNTCGFIDDAVKESLDAIGEALAENGKVIVTGCLGAKPGKAGG
                     DSLIREVHPKVLAVTGPHATQEVMDAVHQYVPKPHDPFVDLVPPQGIKLTPRHYAYLK
                     ISEGCNHRCTFCIIPSMRGDLVSRPVGDVLGEAQRLFEAGVKELLVVSQDTSAYGVDV
                     QCRTGFWDGKPVKTRMLDLCERLGDLAARYGAWVRLHYVYPYPHVDGVIDLMASGRIL
                     PYLDIPFQHAHPAVLKRMKRPGTGERTLERIQEWRAKCPELVIRSTFIAGFPGETQAE
                     FDTLLDFVREAQIDRAGCFAYSPVEGAAANELADQLPQDLREERRARFMQVAEDVSTA
                     KLQRRVGATMQVLVDKAPMLGKKGGVGRSYADAPEIDGLVRLLPPEKVSKQLKVGEFT
                     RARIVATEGHDLVALPI"
     gene            10646..11857
                     /locus_tag="VIN75_23760"
     CDS             10646..11857
                     /locus_tag="VIN75_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY8977250.1"
                     /translation="MSDDGARDAKSTQQIHHGYVPAPGFAAVAPALHRASTVLFKDVA
                     AMRASHWLDKSTYTYGLHGTPTSFTLEARLATLEGGTHAMLAPSGLAAIALVDQSLLS
                     SGDTVLIPANAYFPNKSLAAHELARWGISHRLYDPMDLESLRAALGPDVKLVWIEPPG
                     SVTMEFPDLRGLVRTVRAHAPRATVALDNTWGSGIAFDAFDLDPGASPELGVDVTIHA
                     LTKYPSGGADVMMGAVVTRSQALHDQLALSHCRLGLGVGPSDVELVLRGLQTVALRYA
                     AQDASTRRIAQWALGRKEFARVLHPATPGSPGHEHWAAQCRAAAGLMTLAFDPAIAPD
                     RVDRFVDDLRLFGIGWSWAGPMSLAVPYRPDSGRVGAYAGTLVRLSIGLEDPDDLIRD
                     LEQALDKLNRA"
     gene            complement(12093..12563)
                     /gene="smpB"
                     /locus_tag="VIN75_23765"
     CDS             complement(12093..12563)
                     /gene="smpB"
                     /locus_tag="VIN75_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8977251.1"
                     /translation="MPALIAENRRARFEYHIEETYEAGLVLQGWEVKAIRAGQVQLTD
                     GYVVIRNGELHMIGCRINALRSASTHVNPEADRTKKLLMKGEEIRRLIGKVEQRGFTM
                     VPLNLHFKGSYVKADIALAKGKADHDKRNTIKERDWEREKGRLMRHKVTGPAKG"
     gene            12590..13087
                     /locus_tag="VIN75_23770"
     CDS             12590..13087
                     /locus_tag="VIN75_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY8977252.1"
                     /translation="MKHVKKSVLLWYSAREMYELVVDVESYPKFLPWCESSTILEQHE
                     ADPATGTPAGMTARLGLSYAGVRQSFTTRNEQVQGEFVKLKLVDGPFSNLDGVWQFKP
                     LKKPDPAQPPNPDEPVACKVEFDLAYAFSNRVFEAVLSPVFDQVANTFVDSFVKRAAQ
                     VYGER"
     gene            13077..13427
                     /locus_tag="VIN75_23775"
     CDS             13077..13427
                     /locus_tag="VIN75_23775"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY8977253.1"
                     /translation="MSAEISVQVVWSPSAREVLDVTLRLPDGATVAEAIAAAGWPALA
                     SAARGDAAFAAAGLSAAVWSKGRALDHALRDGDRVEVLRNLQIDPMAARRVRYEAAGG
                     IDALRRRGYAGRKR"
     gene            complement(13462..13995)
                     /locus_tag="VIN75_23780"
     CDS             complement(13462..13995)
                     /locus_tag="VIN75_23780"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8977254.1"
                     /translation="MQKTALSLAACLLALAATGAHAQALWKWRDASGQLHISDKAPPP
                     GTPARNILAAPPGGVMPPPAALVAKPASAAEEPAPGASAAESALDKKKKAVDQDKAAK
                     EAADRAKLEASNAAIRKDNCARAHNALQTLQNGGRMARTNDKGEREYLDDAARADETK
                     RAQDAIAQNCGAAPAGQ"
     gene            14241..>14716
                     /locus_tag="VIN75_23785"
     CDS             14241..>14716
                     /locus_tag="VIN75_23785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8977255.1"
                     /translation="MRLLGKALTFDDVLLVPAYSQVLPRDTSLRTHFTRNIILNLPVV
                     SAAMDTVTEARLAIAIAQEGGIGVIHKNLTAMQQAAEVARVKRYESGLLRDPVTIAPG
                     ARVRDVVELSKLHGVSGFPVVDNGRVVGIVTNRDLRFETRMDAPVREIMTPQERLIT"
BASE COUNT         2045 a         5030 c         5333 g         2308 t
ORIGIN      
        1 gcggccgcgg cggcgcccga ggccgccgac gccggcggcg cgatcagcca cctgggcacc
       61 gccgtggacg cggccgggcg cgcgtccagc gccgcgtcgg cgtggtgcgc cttcttcagc
      121 ggctgctggg gcggcacggg gatggactgg cccaccgcgg cgacggtggt gatggtggcc
      181 gcgacgacgg ccaatgcgac cagaggatgc gtgcgcatgt cgctacacca gcgcgccggg
      241 cgccttgagg tagcgctggg tgatcgcggc ggccgagtgg taggcgaggg cggcgacggt
      301 gccggtcggg ttgtagcccg ggttctgcgg gaaggcgctg gagcccatca cgaacaggtt
      361 ggacacgtcc cacgactgca ggtacttgtt gaccgcgctg gtccgcggat cggcgcccat
      421 gatcgcgcct ccggtgttgt gcgtggtctg gtacggcacc acgtcccagc tgcccgtgcg
      481 cttgttgatc acgagctcgc gcggcttcat cgcgcgggcg atgtcggtgg cgcggtcggt
      541 caggtagtcc gacatccggt gctcgttgtc ggtgaagtcg aacgtcatgc gcagcagcgg
      601 ccggccgtag acgtcgcggt aggtcgggtc gaggtccagg aagttgccgc gatggctcat
      661 cacggcgccg tgcgtcgcga tgctcatcgt cttgaggtag ttggccgcga tggccttctt
      721 gaacgcgctg ccccacttgg gcgtgccgtc gggcaccggg tgcgtctcga tcggccgcgc
      781 gccggtctgc accacgccga ggtagccgcc gccgatgaag cccagccccg agtggtcgaa
      841 gttgtcgccg ttccactcgt cgaccagcat gccgtgcgcg ccggcgccga tgaacggatt
      901 gaggatcctg tcgtcgaaga acaggttcac cgacgaggtg atctggtacg cgtagttctt
      961 gccgatgacg ccctggccgg tctgcgggtc gtacggcgcg ccgatgcccg agagcagcat
     1021 caggcgcacg ttgttcagga tgtacgagca cagcacgatc atgtcggccg gctgctcgaa
     1081 ttcctcgccc gacgcgtcga cgtaggtgac gctgaccgcg cgcttgccgc tcgagtcgag
     1141 gttgaccttc atcacctcgc actcggtgcg cagccggaag ttcggcttct tcatcagcgc
     1201 cggcaggatc gtggtctgcg cgctcgcctt cgaatagttg ccgcaggcga acttctcgca
     1261 gaagccgcag taggtgcagg cctccaggcg cacgccgtac gggttcacgt aggcgcgcga
     1321 catgttggcc gacggctgcg ggaacggatg gagccccagg cccctggccg cctcggcgaa
     1381 cagcgtgggc ggatagctca tcgccatcgg cgggttcggg tactcgttct ggcgcacgcc
     1441 ctcgaacggg ttgccgcccg cctggatctc gccgcgcagg ttgcccgcct tgccgctgat
     1501 gccgcagacc ttctcgaact tgtcgtagtg cggctcgagc tcgtcgtagg tgatgcccca
     1561 gtcctggatg gtcatcgtgt cgggcagcgc cgcggcgccg tagcgctgct cgttgtggct
     1621 gcgggcgacg aagtcggtgg gcaggaagcg ccaggtctgg ccgttccagt ggacgccggc
     1681 gccgcccacg ttggtgccgg gcaggtagga gccgagcctg cgcatcggca gcgccacctg
     1741 gctcgcgtcg ttgcggatcg tctgggtgtc gtgcgcggcg gcctggaaca ggtcgtggcg
     1801 ccagtagtag cgcagctcgt cctgcgcgaa cgtggtggcg aactcgcgcg aggtgtcggc
     1861 ccacgcgccg cgctcgagcg ccagcacgtc gattccggcc tgcgtgagct cctgcgccat
     1921 gatcgcgccg gtccagccga agcccacgag caacgcgtcg accgaaggaa gcttcctggc
     1981 catgtcagct cctcgtccac tcgacgcggc cacggatgcc cactggcggc agcgggtact
     2041 tctcgccgtg cttgccgacg aactccgact ggtcgtagcg cgcgccggga aagccgatga
     2101 gcttccagcc caccatgtcg cggttgccgc cgtagaccgg atcgctccag aagccctcga
     2161 gcgtgttctg cagcagctgc ttgaagaact ccttggcgtc gacgccgtcg agcttcgcgt
     2221 cgccgctctc gagcttcttg aggaaggcgt cctggtcggc cgccgcgagc ttcgcgaacg
     2281 tggcggcctt gccctcgcgc gccacgacgg cgtcgatggc cttgatcgcc gcacggtaca
     2341 tgccggccgg cgtcaaccgc gtctgccagc cctgcgtgga cgtgccgggc ccccatggcc
     2401 cctgcatgta ccagcgctcg ccgcgaccgt aagggcccgc gagctggcgg tcgatgaaga
     2461 cgggcacgcc ggcttcgacg gcgcccggtc caaggtcgtc ggcggggatc agacggcccg
     2521 tcgcggccga gacgaaggcc gcctcggcgt cggtgaacca ggtccagccg gaggtgttct
     2581 ccacgccgac gggcgcgttg gcatggcctt ccttgtccgg cacctcgcga ccgccgagag
     2641 ccgcggccgc ggcggcagtg gcgacgggca cggcgccgat gacggcgccc gcggtgagca
     2701 ggcgtcgacg gctgaggcgc ggttcgtcca tggcggtgtc tcctgtcgag ctgggggctc
     2761 ggtggacacc gttgggcaaa cggcgtgcga gggctgatcg cgcgatcgac gccacgcgcc
     2821 ggcgcgcttc agaacgcgag tggctgctcc gccccctgcg ttcggcgttg tcacctttgc
     2881 cgaagcgccg cgcgcgcggc gcgtcacgca catgggtatg cgcgagcgcg cgagacgctc
     2941 agtcgggacg caccatgtcc tcggcgcgca cccacgcgtc gaactgctcg ccggtgagct
     3001 tgcccgaggc gatcgccgcg tcgcgcagct tcaggccctt ggcgtgcgcg gtgcgcgcga
     3061 tctgcgccgc ggcgtcgtag ccgatgtgcg gattgagcgc cgtcaccagc atcagcgagt
     3121 tgtgcagcag ttcggcgatg cgctcgcggt gcggctcgat gccctgcgcg cagtgcctgt
     3181 cgaatccgcg catgccgtcg tccagcagcc gcacgctctg cagcaggttg tggatcatca
     3241 cgggcttgta gacgttcagc tcgaagttgc cgctggcgcc ggccatgccg accgcgacgt
     3301 cgttgccgat cacctggcag cacagcatcg tcagcgcctc gcactgcgtc gggttgacct
     3361 tgcccggcat gatcgagctg cccggctcgt tctcggggat ctgcaactcg cccaggccgc
     3421 tgcgcgggcc gctcgcgagc cagcgcacgt cgttggcgat cttggtcagc gccgccgcca
     3481 gcgccttgag cgcgccgtgc gcgcgcacga tcgcgtcatg cgccgcgagc gcggcgaact
     3541 tgttgggggc cgtgacgaag cccaggccgg tcatgcgatc gagttcggcc gccacgtcgg
     3601 cgccgaacga ggccggcgtg ttcaggccgg tacccaccgc cgtgccgccg agcgccagct
     3661 cgcgcagctc gggcagggcg gcgtgcagcg cgcgctcggc gtgatcgagc tgcgcgaccc
     3721 agccggagat ctcctggccg agggtcagcg gcgtcgcgtc ctgcaggtgg gtgcggccga
     3781 tcttgacgat gccgtcgaac gccctggcct tggcgtccag cgtcgcgcgc aggccctgca
     3841 gcgccgggat gacgtgctgc gcgaggccct gcacggcggc cacgtgcatc gcggtgggga
     3901 acacgtcgtt ggacgactgg ccgaggttga cctcgtcgtt cgggtgcacc aggcgcttca
     3961 ggcccgtcgg gccgcccagc agctgcgagg cgcggttggc cagcacctcg ttcatgttca
     4021 tgttgctctg cgtgcccgag ccggtctgcc agaccgacag cgggaactcg gcgtcccagc
     4081 ggccctcgat cacctcggcc gcggcgcgcg cgatcgcgtc ggccttggcc gcaggcagcc
     4141 ggccggcgcg ctcgttgacg cgcgccgccg cgctcttgac cagcgcgagc gcctgcagca
     4201 gctcgggcgg catgcgctcg gtggagatcg cgaagtgctc gatggagcgc tgcgtctggg
     4261 cgccccacag gtggtcggcc gggacgggga tgtcgcccca ggcgtcgtgc tcgatgcgtt
     4321 ggtcggtgct catggaatgt cttcgtggaa aggggggctc cccgccagcg tacaggcctt
     4381 gcgaagaccc tgtgcgcatt cggccgggga ttccccttgc tgcaccgcag cgggccgcct
     4441 ctagaatcga tgagcaatca acccaccggg gacactccat ggtcacgacg cgtcgcacca
     4501 ccaaggccag cgccgatccg gcagcgcccg agggcctgcg catcctcaag aagtacccca
     4561 accgccggct ctacgacacg ctgaccagtt cgtacatcac gctggccgac gtcaagaaga
     4621 tggtgctcga gggcgccgcc ttcgaggtgc gcgacgccaa gacgggcgac gacctcaccc
     4681 gctccatcct gctgcagatc atcctcgagg aggagaccgg cggcatgccc atgttcaaca
     4741 cccagatgct ggcgcagatc atccgcttct acggccatgc gatgcagggc gtcatgggct
     4801 cgtacctgga gaagaacctg gccgcgctgg ccgacatgca ggcgcgcttc gccgaaggcg
     4861 gcaagggcct gtacgacgcc acggcgctga cgcccgacgg ctgggccaag ttcatgagcg
     4921 gccaggcgcc gatgctgcag aacctgatgg gcagctacct cgagcagtcc aagaagatgt
     4981 tcgagcagat gcaggagcag atgggcaagc cgggcgaggc gatgttcccg atcccggggt
     5041 tcccgccggc caagcgctga cctcggcctg atggatcgtc gaggaggagg gcgcgcatgg
     5101 cccgcgtcct gatcgcctgg gagttcgtcc gcaacctcgg ccacgtcgcg cgcgacctgc
     5161 cgctcgctcg cgcgtgcccg cggcgcgcgc gctcgggctg ccggcgatga tcctcggctc
     5221 gggcttcatg gtgccggcgc cgggcgtgcc ccagccaacg agcagtacct ggcgggccag
     5281 cggctgcagg cctatggcgc cgggctggtg gtgccggggc gcgcgtcgac gccggaatac
     5341 gcgggcatgc tggagcggtt gctgtcggag ccggccttca ggcagcaggc gcaggccttc
     5401 gcacgcaagt acgccggtca cacgcgcgac agcaccgcgc gcaccgtgtt cgagatcctg
     5461 cagggcctgc tgccgcgcgc ggcctgaccc gcggccggtg cgcgcgcatc agccgcgcgg
     5521 cccgaaacgc gccacggacc gcgccttggc cttcgcggcc tcgacctcgc ggttgtgcgg
     5581 cggcgccgtc gtcaccatcg acgcgatcag cgcgcgcgcc gaggcggcca cttcgtcgac
     5641 ggcccgctcg aacgccgcct cgttggccct ggacggcacc gtgaagccgc tgagcttgcg
     5701 cacgaactgc agcgaggcgt cgcggatctc gatctcggtg gccggcgggt cgaagttgaa
     5761 gagcgtccgg atgttgcggc acatggcggc gatgcgggtt gatgcgaacc atgccgatgg
     5821 tacgcatcct ctggccggga aggcgcaaac cgcgcgaatc ccgcgcggcc tggccagcgg
     5881 cgatcggccg ggcggcgacc gcggtctcag cccaggatcg gctccaggcg ccggatgccg
     5941 tgcacggtgg cctccatgcc gtcgtggtgc gcttcgtaga gctccagcca tcgcgtgaac
     6001 gcatcgggcg tcagcgtggc ggtgagcggc gggcggtcgc cgggcagcgt ggcgagcgcc
     6061 gattccagcg ccgcgatgtc ggcgcgcgcg aagcagtcgt cgatgcgcgg ttgcagttgc
     6121 ggccgcgccg cgtcgacgcg ctggagatag cgaccgatgt ccacgggggc gtccaggtcc
     6181 tcgtcgtcgt cgaagcccac catctcgtcg aagcccggca ggcgggccgg cggtggcgcc
     6241 gtcgtgccgg cggccgctgg tgcggcgtcg agcgggccgt cgaagccgta gacgccctgc
     6301 agcgccgacc agtcgaacgt ggccggatcg gggtggttca gcgcgtgcac gagcgcctcg
     6361 ttggcggcgc gctgttcgag cgtcggctcg ggccgtggcg cggcggccct gtcgagcgct
     6421 cgcagcagcg cgatctccgc gatgctgttc gcgttgaggt acaggtgttc gaagcgcgtg
     6481 cggtcgaggg tcgtcatggt gcgagtgtcg cgcagcgctt cagagcaccg agccggtgag
     6541 cgacgacgcg cccgcgatga ccagcggcag cgcatcgccg gtggtgccgg cgaggaacag
     6601 cgcctgcacg gcgatcggcc tgaggacgcg catcatcgtc tcgaggatcg cccggaccac
     6661 ctgggccgtg atgtgcgccg cgctcttgat cacgacgccc gcccagccgg cggcctggcg
     6721 catggcctcg cagatgtcgg cgtccagatc ggccatccgg ctcatgttcg acgcgagcac
     6781 cgccatgaac aggtcgaccg cggtgaacgc cgtcacgccg atgaggccgg cggccgacaa
     6841 cagtccgctg agcgccttca tcaggtagct caggcccttg gcggtctggc gcgtgatcca
     6901 gttggggttg tccgagctgc gcagcagcca gcgcgccatc accgcgttgt cgtggtcgac
     6961 ggcggcgccg agcgcgcgga cgctgtccca gctcatgcgg tcgcgcgaga cgcgcagcac
     7021 gtactcgttc atgtcgtggt tggcggtgct gagcaggcgg ccggtcggcg acgtcatcat
     7081 catgttgttg acgtcgtcgg gattggggtt caggtggccg aacgggtagg gaccgaccat
     7141 ggtgaccggg tcgaggtcgt gcgagacgcg gtagacattg agcttgccca gcgcctgctc
     7201 catcgcgcgc tgcgcgccga acgcgcccac gcgcgggcag ccgaaggtgt agagcttgac
     7261 ggccttgccc cgctcggcat agtgcgccgc gaccagcgtg gcgaccgcgc cgccgaggct
     7321 gtggcccacg caatgcacga tgtcggcgtc catgacgtgc ctgtcgtcgg aggccaggcc
     7381 gccgcggatg ctgtcgaacg tgttcttgaa gcccgtgtgg acgaggccgt agccgccgaa
     7441 gccggtgagc gagccgtggg cgtcggtgta gaggtcggcc ttgctgtgct cggcgcgggt
     7501 gccgcgcgtg gcgacgatca cgtggcgcga gttgccgcgg ttgaaccgca ggatgtagcc
     7561 gaaccccgtg cgcaggccgg ccgtcgaacc ctggaacacg cgatccagcc gggcgccccc
     7621 gacgtcggtg ccgcgcagcg tggtgttggc gccgccggcc ttctgctggg cgctcttgcc
     7681 gtcgcccagc accatctggc gcacgttggc gcgcgtctca acgcccgcca cggcggtgcc
     7741 ggtcatccaa tccttgagcg tgaagtagca gttgtgggcg atgtaggccg cctcgcgcgg
     7801 cgaaaggctg tcgtggtcgt tgaccgtcat gtgttttcaa gcggcgcgag ccgcggtctc
     7861 cctgtcgatg tttggatcaa agcaccttgg cgaggtccag cgaccagatc gcttcgcgcg
     7921 gcttggcgcc gcggtcgtcc gaggtcctga gccacagcgt cttgcccacg aggccccact
     7981 ggcggacgtt gccgtcgtcc aggcccagct tgagcacggg cacgccgtcg cgatgcgcgc
     8041 tcgagaacag catgaggtgg tcgaagcggt tcacgtagcg ctgcggcccc aggcgctcct
     8101 tgtcggaatg gaacagcacc agcacggcgt ccggggtgac ctgcaaggtg tcgacgaact
     8161 tgaacggcag gccttgcgac cagtcgggcg tgacgccttc ggcgagcagg gcaaggcggt
     8221 cggcggcgag gtcgtagacg tacaggccgt cggtgggcac gggcttgtcg caggtcaggt
     8281 ggatcagcac gcgcccggcg ggcagggcgg tcaccgactc ggcgtcgaac gcgacccagc
     8341 gggcctggtc gggcgagaac tgcgcggcgc gccggctgcc gaaccgcagg tcgtcgtgcg
     8401 gcgccatctg ccacaactgc gtctcgcgcc ggggctcgta gatgttgacg accgccacgc
     8461 cagggtgccc ggcagaaccc ggtgcgacat aggcgcgtat cggctggctg tccgattcct
     8521 ggggcgtcag cttgccggca ggcggcaacg cgtcgccgcc aaggtcgcgg acctgcggat
     8581 agcccggcgt ggccggcgcc gtgaacgtgg ccggcagggc cggctggcgc acgagcggcg
     8641 caccgaccga ggcgtcgacc accgagcttg tcttgccctt gaacagtccg aggatcatct
     8701 tgtcgttccc gctttcgttg gaatgcgcct ggccgcatcc cgcgaccatc gtggccagtg
     8761 tcgctgctgc gagcgaaccc aggaatgcgt gcgcgcagcg cgggaggccc ggaggcggtt
     8821 ttccgaaaag catggtggaa gctggggtga gtgggatccg ggcaggatct cgcgactcgc
     8881 ctcggttcga tggcgcgccc gggccgaggc tccggactct gcagcgctgg cgctgcgaac
     8941 gacttcgaga ccgaaccggc cgacgggccg gcgcccgctg ggtgccgagg gcgattctgc
     9001 tgcatcgcgg cgcgcgccgc cgacccccat tctgggggaa tcgcgtaacc aagtgcgtgc
     9061 cgcagggcgc catgccgggg tggccggagg tcgatggcag gcgccgggct ggcgcacgaa
     9121 ctgcggcggg tcggctggac gcgcgtcctg accgcccgag cgccgggtgc tgcgcgattt
     9181 cccgcggccg gctacaatga cgggttcgcc gtgggctggt tccactgcaa gaaagcccaa
     9241 tggaatcaac gacttacccc accactgaaa ctccggccgg cggcaaggag ccgacgatcg
     9301 cgttcgtctc cctcggctgc ccgaaggcgc tgaccgactc ggagctgatc ctgacccagc
     9361 tgcgcgccga gggctacggc accagcaaga gctactcggg cgcagacctc gtggtggtca
     9421 acacctgcgg cttcatcgac gacgccgtca aggagagcct ggacgcgatc ggcgaggctt
     9481 tggccgagaa cggcaaggtg atcgtcaccg gctgcctggg cgccaagccg ggcaaggccg
     9541 gtggcgacag cctgatccgc gaggtgcacc cgaaggtgct ggccgtcacg ggtccgcacg
     9601 ccacgcagga ggtgatggac gcggtgcacc agtacgtgcc caagccgcac gaccccttcg
     9661 tcgacctcgt gccgccgcag ggcatcaagc tcacgccgcg ccactacgcc tacctgaaga
     9721 tcagcgaggg ctgcaaccac cgctgcacgt tctgcatcat cccgagcatg cgcggcgacc
     9781 tcgtctcgcg gcctgtcggc gacgtgctgg gcgaggcgca gcgcctgttc gaggcgggcg
     9841 tcaaggaact gctggtcgtg agccaggaca cctcggccta cggcgtcgac gtgcagtgcc
     9901 gcaccggctt ctgggacggc aagccggtca agacccgcat gctcgacctg tgcgagcgcc
     9961 tgggcgacct ggccgccagg tacggcgcct gggtgcgcct gcactacgtg tacccgtacc
    10021 cgcatgtgga cggcgtcatc gacctgatgg cctcgggccg catcctgccg tacctggaca
    10081 tcccgttcca gcacgcccac cccgcggtgc tcaagcgcat gaagcgcccg ggcaccggcg
    10141 agcgcacgct cgagcgcatc caggagtggc gcgccaagtg ccccgagctg gtgatccgca
    10201 gcaccttcat cgccggcttc cccggcgaga cgcaagccga gttcgacacg ctgctggact
    10261 tcgtgcgcga ggcgcagatc gaccgcgcgg gctgctttgc ctactcgccg gtcgagggtg
    10321 ccgccgccaa cgaactggcc gaccagctgc cgcaggacct gcgcgaggag cgccgcgcgc
    10381 gcttcatgca ggtcgccgag gatgtgtcca ccgccaagct gcagcgccgc gtcggcgcca
    10441 cgatgcaggt gctggtggac aaggcgccca tgctgggcaa gaagggcggc gtcgggcgct
    10501 cgtatgccga cgcaccggag atcgacggcc tcgtgcgcct gctgccgccg gaaaaagtgt
    10561 ccaagcagct gaaggtgggc gagttcacgc gcgcccgcat cgtcgccacc gaaggccacg
    10621 acctggtcgc actgccgatc tgacgatgag cgacgacggc gcccgggacg cgaagtccac
    10681 gcagcagatc catcacggct acgtccccgc acccggcttc gccgcagtgg cgccggcgct
    10741 gcatcgggcg tcgacggtgc tgttcaagga tgtcgcggcg atgcgcgcca gccactggct
    10801 ggacaagagc acgtacacgt acgggctgca cggcacgccg acgtcgttca cgctggaggc
    10861 gcgcctggcc acgctggagg gcggcaccca cgcgatgctg gcgcccagcg gcctggcggc
    10921 catcgcgctc gtcgaccagt cgctgctgtc cagcggcgac acggtgctga tccccgccaa
    10981 cgcgtacttc ccgaacaaga gcctggccgc gcacgagctg gcgcgctggg gcatctcgca
    11041 ccggctgtac gacccgatgg acctcgagtc gctgcgcgcg gcgctcggcc cggacgtcaa
    11101 gctggtgtgg atcgagccgc ccggctcggt gacgatggag ttcccggacc tgcgcggcct
    11161 cgtgcgcacg gtgcgcgcgc acgcgccgcg ggcgaccgtg gcgctggaca acacctgggg
    11221 cagcggcatc gcgttcgacg ccttcgacct cgaccccggc gcgtcgcccg agctcggcgt
    11281 cgacgtcacg atccatgcgc tcaccaagta cccgtccggc ggggccgacg tgatgatggg
    11341 cgccgtcgtc acacgctcgc aggcgctgca cgaccagctg gcgctgagcc actgccggct
    11401 gggcctgggc gtcgggccga gcgacgtcga gctggtgctg cgcggcctgc agaccgtggc
    11461 gctgcgctac gccgcgcagg acgcctcgac gcgccgcatc gcgcagtggg cgctgggccg
    11521 caaggagttc gcgcgcgtgc tccacccggc cacgcccgga tcgccggggc acgagcactg
    11581 ggccgcgcag tgccgcgcgg cggcgggcct gatgacgctg gccttcgatc cggccatcgc
    11641 gccggatcgc gtcgaccgtt tcgtcgacga cctgcggctg ttcggcatcg gctggtcctg
    11701 ggccgggccg atgagcctgg ccgtgccgta tcgcccggac tcggggcgcg tgggcgccta
    11761 cgcgggcacg ctggtgcggc tgtcgatcgg gctggaggat ccggacgacc tgatccgtga
    11821 cctggagcag gcgctggaca agctgaaccg cgcgtagata ccgtctcgac ccgggccctg
    11881 cgcaatcagg catccgggga cgccagagcc agtacggcag gtcgttcgcg aggcccggcg
    11941 ccgggacgcc atgaagagcg tcatcctgcg cgcagtcgca ggatccacgc accgcgcgat
    12001 gtcgcggtcg gcaggcgtcc tggccggggt ggatcctgcg acttcgcttc gctgcgcgca
    12061 ggatgaccgg gccggatggc ttcccgcttc gctcagcctt ttgccggccc ggtcaccttg
    12121 tgccgcatca gccgcccctt ctcgcgctcc caatcgcgtt ccttgatcgt gttgcgcttg
    12181 tcgtggtcgg ccttgccctt ggccagtgcg atgtccgcct tcacgtagct gcccttgaag
    12241 tgcaggttca gcggcaccat cgtgaatccg cgctgctcga ccttgccgat caggcgccgg
    12301 atctcctcgc ccttcatcag cagcttcttg gtgcggtcgg cctccggatt cacgtgggtg
    12361 gacgccgaac gcagcgcgtt gatgcggcag ccgatcatgt gcagctcgcc gttgcggatg
    12421 acgacgtagc cgtcggtcag ctgcacctgg cccgcgcgga tggccttgac ctcccagcct
    12481 tgcaggacaa ggccggcctc gtacgtctct tcgatgtggt attcgaaccg cgcgcggcgg
    12541 ttctcggcga tgagggcggg catgggggcg cttaaaattc ttccattgta tgaagcacgt
    12601 caagaagtcc gttctcctgt ggtactccgc ccgcgaaatg tacgagctgg tcgtcgacgt
    12661 ggagtcctat ccgaagttcc tgccgtggtg cgagtccagc acgatcctgg agcagcacga
    12721 ggccgatccg gcaacgggca cgcccgcggg gatgacggcg cggctggggc tgtcctatgc
    12781 cggcgtgcgc cagtcgttca cgacgcgcaa cgaacaggtg cagggcgagt tcgtcaaact
    12841 caagctggtg gacggcccgt tctccaatct cgacggcgtc tggcagttca agccgctcaa
    12901 gaagcccgac ccggcgcagc cgcccaaccc ggacgaaccg gtggcctgca aggtcgagtt
    12961 cgacctggcc tacgcgttct ccaaccgcgt gttcgaggcg gtgctgagcc ccgtgttcga
    13021 ccaggtggcc aacaccttcg tcgactcctt cgtcaagcgc gccgcgcagg tctacggtga
    13081 gcgctgagat ctccgtgcag gtcgtctgga gcccgtcggc gcgcgaggtg ctggacgtga
    13141 cgctgcggct gcccgatggc gcgacggtgg ccgaggcgat cgcggccgcc ggctggcccg
    13201 cgctggccag cgccgcgcgg ggcgatgccg cgttcgcggc ggccgggctg tcggccgcgg
    13261 tgtggagcaa gggcagggcg ctcgaccacg cgctgcgcga cggcgaccgc gtggaggtcc
    13321 tgcgcaacct gcagatcgat ccgatggctg cgcgccgcgt gcgctacgag gcggcgggcg
    13381 gcatcgatgc gctgcggcgc cggggctacg ccggacgcaa gcgctgaagc ggcggacggg
    13441 cggcccggcg gcccgcgccg ctcactggcc cgcgggggcc gcgccgcagt tctgggcgat
    13501 ggcgtcctgg gcgcgcttgg tctcgtcggc gcgcgcggca tcgtcgaggt attcgcgctc
    13561 gcccttgtcg ttggtgcgcg ccatgcggcc gccgttctgc agcgtctgca gcgcgttgtg
    13621 ggcgcgcgcg cagttgtcct tgcggatcgc ggcgttgctc gcctcgagct tggcgcggtc
    13681 ggccgcttcc ttggcggcct tgtcctggtc gacagccttc ttcttcttgt cgagcgcgga
    13741 ttctgcggcc gacgccccgg gggccggctc ctcggccgcg gaggcgggct tggcgaccag
    13801 cgcggccggg ggcggcatga cgccgccggg cggggcggca aggatgttcc tggccggcgt
    13861 tcccggaggc ggcgccttgt cgctgatgtg cagttgaccg gaggcatcgc gccacttcca
    13921 gagagcctgg gcatgggcgc cggtggcggc gagcgccagc aggcaggcgg cgagggacag
    13981 ggcggtcttt tgcatggagt tttgcgcccg ggcgaggtgc gggcggagcg agtgttgtgg
    14041 acccaacgtc gtgccggcgc ggcacgttga cattctcgtg tcatcggcag cgccgccggc
    14101 cagtgtagtg ctctgcagtg gtgacaaatc gcaaactaat cgagcgatcg gcgccggcct
    14161 gccaggggct tcgcgcgacc ccggccgagc aagaggtgtc gtggtgcgta taatccgctt
    14221 ttgcgtctgg agcttccccc atgcgccttc taggcaaagc gctcaccttc gacgacgtgt
    14281 tgttggtgcc ggcatattcc caggtgttgc cccgcgacac cagcctgcgg acccatttca
    14341 cccgcaacat catcctgaac ctgcccgtcg tctcggcggc catggacacc gtgacggagg
    14401 cccgcctggc gatcgcgatc gcacaggaag gcggcatcgg cgtcatccac aagaacctca
    14461 cggccatgca gcaggccgcc gaagtggccc gcgtcaagcg ctacgaatcg ggcctgctgc
    14521 gcgacccggt cacgatcgca ccgggcgcgc gcgtgcgcga cgtcgtcgag ctgtccaagc
    14581 tgcacggcgt ctcgggcttc ccggtggtgg acaacggccg cgtcgtcggc atcgtcacca
    14641 accgcgacct gcgcttcgag acccgcatgg acgcccccgt gcgcgagatc atgacgccgc
    14701 aggagcgcct gatcac
//
LOCUS       DATCRO010000874         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_145034, whole genome shotgun sequence.
ACCESSION   DATCRO010000874 DATCRO010000000
VERSION     DATCRO010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145034"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VIN75_23790"
     CDS             <1..442
                     /locus_tag="VIN75_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977256.1"
                     /translation="DMDGRSVPLTTLKGQWLLVVVAPAACGKACERNLFLQRQLREMM
                     GAERDRIQKVWLVDSPEPVAPALAAAMAAPPTVLSYRVDRAALEHWLEPEHGHALEDH
                     LYIVDPLGNWMMRTPPDPDPVKLKKDVERLLRASQWWNRPKAAQ"
     gene            504..1613
                     /locus_tag="VIN75_23795"
     CDS             504..1613
                     /locus_tag="VIN75_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY8977257.1"
                     /translation="MTQTPAYAWDSLPGLVLIALGLVSIVLMISWWRGGLRDKSRLQR
                     VAALGGLTLFLCFDLVVFGAYTRLSDSGLGCPDWPGCYATSSPVAAAEHIARAQALAP
                     EGPVTEKKAWIEMIHRYFAGTLGLLIALMLVAAITSRDRRARLLIGLTLAWYIGQAFF
                     GALTVTWKLYPAIVTGHLLGGMGLLALLALQAPARADDAARRTALDRWALGLAALALL
                     LQITLGGWVSTNYAVLACTGFPQCNGQWWPAMNWSEGFTFARELGRRADGAPIAMTAL
                     VAIQWMHRLFAVVFTAAMGWLAARLWKRGNISRRWAVIMLGVVAVQWATGLSNVVLGW
                     PLVAALAHTAGAAALVICIGFEAAAALNSRIRVSS"
     gene            1610..2518
                     /gene="cyoE"
                     /locus_tag="VIN75_23800"
     CDS             1610..2518
                     /gene="cyoE"
                     /locus_tag="VIN75_23800"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8977258.1"
                     /translation="MSSVPVPNPAPASAQPSRWRQYYVLTKPRVVQLIVFCAVIGMFL
                     AVPGWPPLAPTLAATAGIWLVAAAAAAFNCLVEQEIDRRMTRTAWRPTAKGELTDMQA
                     LLFSALLCGLGAALLLIWVNALTLWLTLATFIGYAVIYTLVLKPNTPQNIVIGGASGA
                     MPPVLGWAAMTGHAEANAWLLCLIIFLWTPPHFWALALYRTEDYRKAGLPMLPVTHGP
                     DFTKLHVFLYTLVLFAGTLLPFVSGLSGWFYLASAVILGATFSAYAFKLWRHYSDELA
                     RRTFRFSILYLSLLFAALLIDHYIPR"
     gene            2515..3117
                     /locus_tag="VIN75_23805"
     CDS             2515..3117
                     /locus_tag="VIN75_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8977259.1"
                     /translation="MNTRRLFVSLAAAGVLALSGCERIAPPHHFNAIDLTGAKYAQGF
                     DLPDFDGRTRTLADFKGKVTVVFFGYTQCPDACPTTMAELSGILKTLGPDASKVQVVF
                     ISVDPSRDTPALLKSYVTNFRPDFIALRGDEAQTQQIIKDFKLIVQKVPGRTPDSYTI
                     DHTAGTYIFDPEGRIRLFASQSLDPALLTEDVKALLAEKA"
     gene            complement(3233..>4284)
                     /locus_tag="VIN75_23810"
     CDS             complement(3233..>4284)
                     /locus_tag="VIN75_23810"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY8977260.1"
                     /translation="ARRRLLMAARRQRLADDPTLTVLWPDEHAAAPDAAALPDARLRL
                     LFVCAHPAIDPGLHSALMLQTVLGLDAARIASAFLVKPEAMTKRLVRAKAKIRDAGLR
                     FEEPGPDEWPERLASVLEAIYGAYTLHWGLADDADAGELAGEARFLAELVASQLPGEP
                     EALGLVALLWLCEARRPARRTEYRPLHEQDPRDWDAALIAQADAALHEAATHRAPGPL
                     QLEAAIQAAHTQGRLDGHVPWDGISRLYERLLEIAPTVGARIAHAVALAHARPDAREG
                     LALLEDIDAVLVSAHQPWWAARAHLLAMAGLHGEAATAYGRALALTVEPRLRAWMMER
                     LRAEEAADGGDGARS"
BASE COUNT          565 a         1567 c         1488 g          664 t
ORIGIN      
        1 ggacatggac ggtcgctcgg tgccgctgac cacgctcaag ggccagtggc tgctggtcgt
       61 cgtcgcgccc gcggcctgcg gcaaggcctg cgagcgcaac ctgttcctgc agcgccagct
      121 gcgcgagatg atgggcgccg agcgcgaccg catccagaag gtctggctgg tcgacagccc
      181 cgaacccgtc gcacccgcgc tcgccgccgc gatggccgcg ccgccgacgg tgctgagcta
      241 ccgcgtcgat cgcgccgcgc tggagcactg gctcgagccc gagcacggcc acgcgctgga
      301 ggaccacctc tacatcgtcg acccgctggg caactggatg atgcgcacgc cgcccgatcc
      361 ggatccggtc aagctcaaga aggacgtcga gcggctgctt cgcgcgtcgc agtggtggaa
      421 caggccgaag gccgcccaat gagcgcgcga cagccgcgag agcagggcgc tcctggccgc
      481 agccgccagg cggaggccgg gcgatgaccc agacgcctgc ctacgcctgg gactcgctgc
      541 ccggcctcgt gctcatcgcg ctcggcctcg tgtcgatcgt gctgatgatc tcgtggtggc
      601 gcggcgggct ccgcgacaag tcgcggctgc aacgcgtggc cgcgctcggc gggctgaccc
      661 tgttcctgtg cttcgacctc gtcgtcttcg gcgcgtacac gcgcctgagc gattcgggcc
      721 tgggctgccc ggactggccg ggctgctatg ccacgtccag ccccgtcgcg gcggccgagc
      781 acatcgcccg cgcgcaggcg ctggcgcccg aggggccggt caccgagaag aaggcgtgga
      841 tcgagatgat ccaccgctac ttcgcgggca cgctcggact cctgatcgcg ctcatgctgg
      901 tcgccgcgat cacgtcgcgc gaccgtcgcg ccaggctgct catcggcctc acgctggcct
      961 ggtacatcgg ccaggcgttc ttcggcgcgt tgaccgtgac gtggaagctc taccccgcca
     1021 tcgtcaccgg ccacctgctg ggcggcatgg gcctgctcgc gctgctggca ctgcaggcgc
     1081 cggcgcgcgc cgacgatgcc gcgcggcgca ccgcgctcga ccgctgggcg ctcggcctgg
     1141 cggcgctcgc cctgctgctg cagatcacgc tcgggggctg ggtcagcacc aactacgcgg
     1201 tgctcgcctg cacgggcttc ccgcagtgca acggccagtg gtggcccgcg atgaactggt
     1261 cggagggctt cacgttcgcc cgcgagctcg gccggcgcgc ggacggcgcg ccgatcgcga
     1321 tgaccgcgct ggtggccatc cagtggatgc accgcctgtt cgccgtcgtg ttcaccgcgg
     1381 cgatgggctg gctcgcggcg cggctttgga agcgcgggaa tatttcgcgg cgctgggctg
     1441 tcatcatgct gggtgtggtg gcggtccaat gggccaccgg cctgtcgaac gtcgtgctgg
     1501 gctggccgct ggtggccgcc ctggcgcaca ctgccggggc cgcggccctg gtgatctgca
     1561 tcggcttcga ggccgccgcc gcgctgaatt cgagaatccg ggtttcgtca tgagttccgt
     1621 tcctgtcccc aaccccgcgc ccgcgtcggc ccagccgtcg cgctggcgcc agtactacgt
     1681 gctcaccaag ccgcgcgtgg tgcaactcat cgtgttctgc gcggtgatcg gcatgttcct
     1741 ggccgtgccg ggctggccgc cgctggcgcc cacgctggcc gccaccgcgg gcatctggct
     1801 cgtcgccgcg gccgcggccg cgttcaactg cctcgtcgag caggagatcg accgccgcat
     1861 gacgcgcacc gcctggcgcc cgacggccaa gggcgagctc accgacatgc aggcgctgct
     1921 gttctcggcg ctgctgtgcg gcctgggcgc cgcgttgctg ctgatctggg tcaacgcgtt
     1981 gacgctgtgg ctcacgctgg ccaccttcat cggctacgcc gtcatctaca cgctggtgct
     2041 caagcccaac acgccgcaga acatcgtgat cggcggcgcc tcgggcgcga tgccgccggt
     2101 gctcggctgg gccgcgatga ccggccatgc cgaggccaac gcctggctgc tgtgcctgat
     2161 catcttcctg tggacgccgc cgcacttctg ggcgctggcg ctttaccgca cggaggacta
     2221 ccgcaaggcc ggcctgccca tgctgcccgt cacccacggg cccgacttca ccaagctgca
     2281 cgtcttcctg tacacgctgg tgctgttcgc cggcacgttg ctgccgttcg tcagcggcct
     2341 gagcggctgg ttctacctgg ccagcgcggt catcctgggg gcgacgttct cggcctacgc
     2401 gttcaagctc tggcgccact acagcgacga actggcgcgc cgcacgttcc gcttctcgat
     2461 cctgtacctg tcgctgctgt tcgcggcgct gctgatcgac cactacatcc ctcgatgaac
     2521 actcgccgac tcttcgtgtc gctggccgcc gcgggcgtgc tggcgctctc cggctgcgag
     2581 cgcatcgcgc cgccgcacca cttcaacgcg atcgacctga ccggcgccaa gtacgcccag
     2641 ggcttcgacc tgcccgactt cgacggcagg acgcgcacgc tggccgactt caagggcaag
     2701 gtcaccgtcg tcttcttcgg ctacacgcaa tgtcccgacg cgtgcccgac gacgatggcc
     2761 gagctgtcgg gcatcctcaa gacgctgggc cccgacgcct cgaaggtgca ggtggtcttc
     2821 atctcggtcg acccgtcgcg cgacacgccg gcgctgctca agagctacgt caccaacttc
     2881 cgccccgact tcatcgcgct gcgcggcgac gaggcgcaga cgcagcagat catcaaggac
     2941 ttcaagctga tcgtgcagaa ggtgccgggc aggacacccg acagctacac gatcgaccac
     3001 acggccggca cgtacatctt cgatcccgaa ggacggatcc ggctgttcgc gtcgcagagc
     3061 ctggatccgg cgctgctgac ggaggacgtg aaggcgttgc tggccgagaa ggcctgacgc
     3121 ccgagtccgt catcgtgcgt gcgccaacaa ggagcccgcc aacagcgggg tcaggtcttg
     3181 aaagttgcgc gctcaggcgt gaccaggttg cgatgcgccg ctcgcgcaac tttcaagacc
     3241 tggccccgtc tccgccgtcc gccgcttcct ccgcccgcaa ccgttccatc atccacgccc
     3301 gcagccgcgg ctcgacggtc agcgcgagcg cgcggccgta cgccgtcgcc gcctcgccat
     3361 ggagtccggc catcgcgagc aggtgcgcgc gggcggccca ccagggctgg tgcgcggaga
     3421 cgagcacggc gtcgatgtcc tcgagcaacg ccagcccctc gcgcgcatcc ggccgtgcgt
     3481 gagccagcgc caccgcgtgc gcgatgcgcg cgccgacggt cggcgcgatc tcgagcaggc
     3541 gctcgtacaa gcggctgatg ccgtcccagg gtacgtggcc gtcgaggcgg ccctgcgtgt
     3601 gcgcggcctg gatcgcggcc tcgagctgca acggaccggg tgcgcgatgc gtcgcggcct
     3661 cgtgcagcgc ggcgtcggcc tgcgcgatca gcgcggcgtc ccagtcgcgc gggtcctgtt
     3721 cgtgcagcgg ccggtactcg gtgcgccgcg cgggacgccg cgcctcgcac agccacagca
     3781 gcgcgaccag gcccagcgcc tcgggttcgc cgggcagctg cgaagcgacc agttcggcga
     3841 ggaagcgcgc ctcgcccgcc agctcgccgg catcggcgtc gtcggccagg ccccagtgca
     3901 gcgtgtaggc gccgtagatc gcctcgagca ccgaggcgag ccgctcaggc cattcgtcgg
     3961 ggccgggctc ctcgaagcgc aggcccgcgt cgcggatctt ggccttggcc cgcaccaggc
     4021 gcttggtcat cgcttcgggc ttgaccagga aggcactggc gatgcgcgcc gcgtccaggc
     4081 ccagcaccgt ctgcagcatg agcgcgctgt gcaggccagg atcgatcgcc gggtgcgcgc
     4141 agacgaacag cagccgcagc cgcgcgtcgg gcagcgccgc ggcgtcgggt gcggcggcgt
     4201 gctcgtcggg ccacagcacg gtcagcgtcg gatcgtcggc caggcgctgg cggcgggcgg
     4261 ccatcagcag gcggcgtctg gcgg
//
LOCUS       DATCRO010000875         7532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_122156, whole genome shotgun sequence.
ACCESSION   DATCRO010000875 DATCRO010000000
VERSION     DATCRO010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122156"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VIN75_23815"
     CDS             <1..727
                     /locus_tag="VIN75_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY8977261.1"
                     /translation="GFFARHTVAPVLIAAGTASALVYVMVDPKPLYVTSPIAMLPSSI
                     LFTLVASVAFGGFGWGFLLLVERSRALFARLHSPALRLGVGGLLVGALSAFVPEVWGN
                     GYSAVERALAGTEPWQWLALVLVTKVVATSWSSGSGAIGGMFTPSLFVGAAAGGVLAQ
                     LAALWLPTAWVGDPRSLVVIGMAAVLTAATHAPLMAITMVLEMTNEFQLTVPVMLACA
                     VSYAISTQFGAKPLYGNPIEAVR"
     gene            847..1302
                     /gene="aac(6')"
                     /locus_tag="VIN75_23820"
     CDS             847..1302
                     /gene="aac(6')"
                     /locus_tag="VIN75_23820"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HEY8977262.1"
                     /translation="MRFTICPCEHADDAGWLALRQALFPHHLESTLRAEMARFVAQPQ
                     RYAQFLAIDGEGVPVGLVEVALRGDPVPGTRSTPVAFLEAIYVLPQARRRGLARALVG
                     HARAWGRARGCSEFASDALLANEESHAMHRALGFDETTRIICYRQAIDG"
     gene            1405..2280
                     /locus_tag="VIN75_23825"
     CDS             1405..2280
                     /locus_tag="VIN75_23825"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977263.1"
                     /translation="MTKHLFKPATLVLGLALAAPLPALSAVSVSVTIAPPPLPLYAQP
                     PIPGDGYIWTPGYWAWDPATGDYVWVPGTWVMPPSVGLLWTPGYWAFFDGGYHWHRGY
                     WGPRVGYYGGINYGYGYIGHGYEGGRWDRGRFRYNTAVNNIPSGRVHAVYRAPVPQRP
                     AHPESFNGGSSPWHSQPTASQRRFEGGHHPDWTQAQQEHEHRAFGTPEQRMGSNHGMP
                     PTAATPRPGGFGEPQVERARTAPEMRAQPVRPGPAMRPAEAPHLEAAPRGEQHAQPRG
                     ENRGHPGGNRGDDHR"
     gene            complement(2284..3525)
                     /locus_tag="VIN75_23830"
     CDS             complement(2284..3525)
                     /locus_tag="VIN75_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8977264.1"
                     /translation="MTSDYSTKTRDLLQRLGAFFDQHIHPNEERYVREVDENRRAGNA
                     WIPSKVVDELKPIARREGLWNLFLPHPYEGQGGISNLDYAPLCEQMGRVMWSSEVFNC
                     SAPDTGNMETLERYGTQEQKARWLRPLLAGEIRSAFLMTEPAVASSDATNIQCSIRRE
                     GDEYVINGRKWFSSGAGNPRCAIFIVMGKTDPDGPRHAQQSMVLVPRDTPGVTVVRPL
                     TVFGYDDAPHGHMEVTLENVRVPVANILLGEGRGFEIAQGRLGPGRIHHCMRSIGTAE
                     RALESMVDRLTERVAFGRPLSEQSIWHERIAESRCLIDQARLLTLNAAHRMDTVGNKE
                     ARAEIAMIKVVAPNVSCKVVDWAIQAHGAAGVSGDFWLADAYAQQRTVRIVDGPDEVH
                     RNAIAKLEIARRAAARRPHAG"
     gene            complement(3676..5487)
                     /locus_tag="VIN75_23835"
     CDS             complement(3676..5487)
                     /locus_tag="VIN75_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8977265.1"
                     /translation="MTRPIEDYALIGNQETVALVARDGSIDWMGMPRFDSPPCFAALL
                     GEPGHGRWRVAPADDAATVSRCYRDGSTVLETRFTTPAGEVVLIDFMSRRDGVCDLMR
                     LVRGVRGRVAMRTEIIVRFDYGSVVPWVSRQDDGRLHFTAGPDRLTLATPVPLRGEDM
                     RTLGDFEVAPGEEVAFTLTWQPSWRDDLPAPDASDALDQVVSQWASWSSKLPQGDDVS
                     EAMRRSLVTLKALAHWQTGGIIAAATTSLPEKIGGTRNWDYRFCWLRDATFTLYALLN
                     LGFVEEAEAWRAWLVRAVAGSPDDLQIMYGVAGERRLVEYEVPWLPGYEGSAPVRVGN
                     AAAGQVQLDVYGEVLDAMYVGRKAGLAYDATSWSLECAMVKHLETIWDQPDDGIWEVR
                     GGRRHFVHSKVMAWVAFDRAVRSATEFGLEGPVDHWIDVRDAIHAQVCERGFDRSQGS
                     FVQSYGSPSLDASLLLVALVGFLPPDDPRMVGTVAAIERRLLRDGLVLRYDTGEGQDG
                     LPPGEGAFLACSFWLADNYLLQDRIDEAQALFDRLLGFRNDVGLLAEEYDPVAGRQLG
                     NFPQAFSHLALINTATGLAGRLGTAHQRAAGSSQTRK"
     gene            5621..6001
                     /locus_tag="VIN75_23840"
     CDS             5621..6001
                     /locus_tag="VIN75_23840"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HEY8977266.1"
                     /translation="MSFLRGRRTLSSWIALVAILVVTLAPSVTGMLSASSGQLWDQVC
                     SATSPAATKTSTSGDGSPAAPHAFEHCPYCALHADLAPPPDPRQAGAAVAVSFRAQPA
                     AFVRAPRARATWASAQPRAPPRVA"
     gene            6119..7204
                     /locus_tag="VIN75_23845"
     CDS             6119..7204
                     /locus_tag="VIN75_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8977267.1"
                     /translation="MTNPLAAAALASARPLTLVSAAAVLALAAADASACATCGCSLST
                     DAATGYPARPGWNMSLQFDYIDQSQLRHGNHAVSTSDVAAINDAGGDQEVEHDTVNRY
                     TTLGIGWMPDEDWGLRLLVPWVDRGHSTYASATNPVTDDQLSTARIKGLGDVRLLVTW
                     QGLLEEHNLGLQLGLKLPTGRYGGASADGSAIVGRKPVAFQHGPLSANPSPDNLVDTS
                     LQPGTGSTDIIVGAFWHQPVSTTVEAFANATFQAAVARRLRGQGQDFRVGNTSFVTFG
                     ARWEATPTLIPQVQVNISHKGADQGALADNPDSEGTVVYLSPGITGTLGEHWQAFGFV
                     QLPVASHLVGYQLAPRWTASAGVSYAF"
     gene            complement(7294..7369)
                     /locus_tag="VIN75_23850"
     tRNA            complement(7294..7369)
                     /locus_tag="VIN75_23850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7334..7336),aa:His,seq:gtg)
BASE COUNT         1083 a         2788 c         2566 g         1095 t
ORIGIN      
        1 gggcttcttc gcgcgccaca cggtcgcgcc ggtgctgatc gccgcgggca cggccagcgc
       61 gctggtctac gtgatggtcg acccgaagcc gctgtacgtc acgagcccca tcgcgatgct
      121 gccgtccagc atcctgttca cgctggtggc cagcgtcgcg ttcggcggct tcggctgggg
      181 cttcctgctg ctggtcgagc gcagccgcgc actgttcgcc cgcctgcatt cgcccgcgct
      241 gcggctgggc gtgggcggcc tgctggtggg cgcgctgtcg gccttcgtgc ccgaggtctg
      301 gggcaacggc tacagcgccg tggaacgcgc gctcgcgggc accgagccct ggcagtggct
      361 cgcgctggtg ctcgtgacca aggtcgtggc gacgtcgtgg agttcgggca gcggcgcgat
      421 cggcggcatg ttcacgccgt cgctgttcgt cggcgccgcg gcgggcggcg tgctcgcgca
      481 gctggccgcc ctgtggctgc ccaccgcgtg ggtgggcgac ccgcgctcgc tggtcgtcat
      541 cggcatggcc gccgtcctca ccgccgccac gcacgcgccg ctgatggcca tcacgatggt
      601 gctggagatg accaacgagt tccagctgac ggtgccggtg atgctcgcct gcgccgtgtc
      661 gtacgcgatc agcacgcagt tcggcgcgaa gccgctgtac ggcaatccga tcgaggcggt
      721 gcgctgaagg cgcgtcgccg catcctgttc caaacggttg cagacgcggg gccggcgcgg
      781 gatcatgctg gatcccatgg ccgggcgagt accccgagcc gtattgatac cgattgccga
      841 acgtccatgc gattcacgat ctgcccttgc gagcacgccg acgatgccgg ctggctggcg
      901 ctgcgccaag cgctgttccc ccatcatctc gaatccaccc tgcgcgcaga gatggcgcgc
      961 ttcgtcgcgc agccgcagcg ctacgcgcag ttcctggcga tcgacggcga aggcgtgccc
     1021 gtgggcctgg tggaggtggc gctgcgcggc gacccggtac cgggcacgcg cagcacgccc
     1081 gttgccttcc tcgaggccat ctatgtgctg ccgcaggccc gcaggcgcgg cctcgcacgc
     1141 gcgctcgtgg ggcacgcgcg cgcgtggggc cgcgcacgcg gctgctcgga gttcgcctcc
     1201 gacgcgctgc tcgccaacga ggagagccac gcgatgcacc gcgccctcgg cttcgacgaa
     1261 accacgcgaa tcatctgtta ccggcaagcg atcgacggct gaagcgtcca ctgacctgcc
     1321 cgacaggctt gccgattgcc gttcctactg cagagcacgc gccgccgggg cgcgtccacc
     1381 acggcatgac tggagggcaa ttcgatgacc aagcacctct tcaaacccgc gacactcgtg
     1441 ctgggcctgg ccctggcagc accgctgccg gccttgtcgg ccgtctccgt ctcggtgacc
     1501 atcgcgccgc cgccgctgcc gctgtacgcc cagccgccga tccccggcga cggctacatc
     1561 tggacgccgg gctactgggc ctgggatccg gccaccggcg actacgtctg ggtgcccggc
     1621 acctgggtga tgccgcccag cgtcggcctg ctgtggacgc cgggctactg ggccttcttc
     1681 gacggcggct accactggca ccgcggctac tggggcccgc gcgtgggcta ctacggcggc
     1741 atcaactacg gctatggcta catcggacat ggctacgaag gcggacgctg ggaccgcggc
     1801 cgcttccgct acaacaccgc ggtcaacaac atcccgtcag gccgcgtgca cgccgtctat
     1861 cgcgcgccgg tgccgcagcg ccccgcgcac cccgagagct tcaacggcgg cagctcgccc
     1921 tggcattcac agcccacggc gagccagcgc cgcttcgagg gaggccatca tccggactgg
     1981 acgcaggcgc agcaggaaca cgagcaccgc gcgttcggca cacccgagca gcgcatgggc
     2041 agcaaccacg gcatgccgcc caccgcggcc acgccgcgcc ccggcggatt cggcgagccg
     2101 caggtcgaac gcgcgcgcac ggcgcccgag atgcgtgcgc agccggtgcg ccccggtccg
     2161 gcgatgcgcc ccgcagaagc accgcacttg gaagcggcgc cacgcggtga acagcacgcg
     2221 cagccgcgcg gcgagaaccg cggccacccg ggcggcaacc gcggcgacga ccaccgctga
     2281 ccgtcagccc gcgtgcggcc tgcgcgccgc cgcccggcgc gcgatctcca gcttggcgat
     2341 cgcgttgcgg tgcacctcgt cgggcccgtc gacgatgcgc accgtgcgct gctgcgcgta
     2401 ggcatcggcg agccagaagt cgccgctgac gcccgcggcg ccatgcgcct ggatcgccca
     2461 gtcgacgacc ttgcacgaca cgttgggcgc caccaccttg atcatcgcga tctcggcgcg
     2521 cgcctccttg ttgcccaccg tgtccatccg gtgcgccgcg ttgagcgtga gcaggcgcgc
     2581 ctggtcgatc aggcagcggg actcggcgat gcgctcgtgc cagatcgact gctcggacag
     2641 cgggcggccg aacgcgacgc gctcggtgag ccggtcgacc atggactcga gcgcgcgctc
     2701 ggcggtgccg atcgagcgca tgcagtgatg gatgcggccc ggcccgaggc ggccctgcgc
     2761 gatctcgaag ccgcggccct cgcccagcag gatgttggcc acgggcacgc gcacgttctc
     2821 gagcgtgacc tccatgtggc catgcggcgc gtcgtcgtag ccgaacaccg tgagcggacg
     2881 cacgaccgtg acgcccggcg tgtcgcgcgg caccagcacc atcgactgct gcgcgtggcg
     2941 cggaccgtcg gggtcggtct tgcccatcac gatgaagatc gcgcaacgcg gattgcccgc
     3001 gccggagctg aaccacttgc ggccgttgat gacgtactcg tcgccctcgc ggcggatcga
     3061 gcactggatg ttggtggcgt ccgacgaggc caccgcgggc tcggtcatca ggaacgccga
     3121 gcggatctcg ccggccagca gcggccgcag ccagcgcgcc ttctgttcct gggtgccgta
     3181 gcgctcgagc gtctccatgt tgccggtgtc gggcgccgag cagttgaaga cctccgatga
     3241 ccacatcacg cggcccatct gctcgcacag cggcgcgtag tcgaggttgg agatgccacc
     3301 ctggccttcg tacggatgcg gcaggaacag gttccacagg ccctcgcggc gcgcgatcgg
     3361 cttgagctcg tcgaccacct tcgacgggat ccacgcgttg cccgcgcgcc ggttctcgtc
     3421 gacctcgcgc acgtagcgct cctcgttggg atggatgtgc tggtcgaaga aggcgcccag
     3481 gcgctgcagc aggtcgcggg tcttggtcga gtagtcgctg gtcatcgtgg cctccaggcc
     3541 ggaatcggat cagccgtatt caatcatcgg cgtcggcgcc gcgtccgtcc cgcacgagac
     3601 accccgtcgc gcacgcgacg ccgcgcgcgt cccgcacgag acagggcccg gcccgggata
     3661 cgctgcgcca gcgcgtcact tcctggtctg gctgctcccc gccgcccgct ggtgcgccgt
     3721 gcccagccgg cccgcgagac ccgtcgcggt gttgatcagc gcgaggtgcg agaacgcctg
     3781 cgggaagttg ccgagctggc gcccggcgac cgggtcgtat tcctcggcca gcaacccgac
     3841 gtcgttgcgg aacccgagca ggcgatcgaa cagcgcctgc gcttcgtcga tgcgatcctg
     3901 cagcaggtag ttgtcggcga gccagaagct gcacgcgagg aacgcgccct cgccgggcgg
     3961 caggccgtcc tggccttcgc cggtgtcgta gcgcagcacg aggccgtcgc gcagcaggcg
     4021 gcgctcgatc gccgcgaccg tgccgaccat gcgcggatcg tcgggcggca ggaagcccac
     4081 gagcgcgacc agcagcaggc tcgcatcgag cgacggcgag ccgtacgact gcacgaacga
     4141 tccctgcgag cgatcgaagc cgcgctcgca gacctgcgcg tggatcgcgt cgcgcacgtc
     4201 gatccagtgg tccaccgggc cctcgagccc gaattcggtg gccgaccgca ccgcgcggtc
     4261 gaacgcgacc caggccatca ccttcgagtg cacgaaatgg cgccgcccgc cgcgcacttc
     4321 ccagatgccg tcgtcgggct ggtcccagat cgtctcgagg tgcttgacca tggcgcactc
     4381 gagcgaccag ctcgtggcgt cgtaggccag gcccgccttg cgcccgacgt acatcgcgtc
     4441 cagcacctcg ccgtagacgt ccagctgcac ctggccggcg gcggcgttgc ccacgcgcac
     4501 cggcgcggag ccctcgtagc cgggcagcca gggcacctcg tattcgacga gccggcgctc
     4561 gccggccacg ccgtacatga tctgcaggtc gtccgggctg cccgccaccg cccgcaccag
     4621 ccatgcgcgc cacgcctcgg cctcctcgac gaagcccagg ttcaggagcg cgtacagcgt
     4681 gaaggtggcg tcgcgcagcc agcagaagcg gtagtcccag ttgcgcgtgc cgccgatctt
     4741 ctcgggcagc gaggtcgtgg ccgccgcgat gatgccgccc gtctgccagt gcgcgagcgc
     4801 cttgagcgtg accagcgacc ggcgcatcgc ctccgacacg tcgtcgccct gcggcagctt
     4861 cgacgaccac gacgcccatt gcgagaccac ctgatcgagc gcgtcggacg catcgggcgc
     4921 gggcaggtcg tcgcgccacg agggctgcca ggtgagcgtg aacgccacct cctcgcccgg
     4981 cgccacctcg aagtcgccaa gcgtgcgcat gtcctcgccg cgcaagggca ccggcgtggc
     5041 cagcgtgagg cggtcggggc cggcggtgaa gtgcagccgg ccgtcgtcct ggcgcgagac
     5101 ccacggcacg accgagccgt agtcgaagcg cacgatgatc tcggtgcgca tcgccactcg
     5161 cccgcgcacg ccgcgcacga ggcgcatcag gtcgcagacg ccgtcgcggc ggctcatgaa
     5221 gtcgatcagc accacttcgc ccgcgggcgt cgtgaagcgg gtctcgagca cggtggaacc
     5281 gtcgcggtag cagcggctga ccgtcgccgc gtcgtccgcc ggcgcgacgc gccagcgtcc
     5341 gtgcccgggc tcgcccagca aggcggcaaa gcatggcggg ctgtcgaagc gcggcatgcc
     5401 catccagtcg atcgagccgt cgcgcgcgac gagggcgacg gtctcctggt tgccgatcag
     5461 cgcgtagtct tcgatgggac gggtcatgcg gctttccatg ttggcaagga ccgactcggc
     5521 cgtggcaacc ggcaggccag ggctgccgca tgatccctct ccctcgaaat cccgggtgat
     5581 cggcatggcc ggcgcatgcg cgccgcccta gaatcgcgcc atgtccttcc tgcgcggccg
     5641 ccgcacgctt tccagctgga tcgccctcgt cgcgatcctg gtggtcacgc tggcgccgtc
     5701 cgtcacgggg atgctgtcgg cctcgagcgg gcagttgtgg gaccaggtct gctcggccac
     5761 gtcgccggct gccacgaaga cgtcgacgtc cggggacgga tcgcccgccg cgccgcatgc
     5821 gttcgagcac tgcccgtatt gcgcgctgca cgccgacctg gcgccgccgc ctgatccgcg
     5881 ccaggccggc gcggcggtcg ccgtttcctt ccgtgcacag cccgccgctt tcgtccgcgc
     5941 gccccgcgcg cgcgccacct gggccagcgc ccagccgcgc gcaccgccgc gcgtcgcctg
     6001 agatcgcccg cgcgggaacg cccgcgaacc gtccgacatc catcgggcat ccattgatct
     6061 cgcgccgaac gcgcccgccg attgcccgcg gacgcgtcgg cccacgcatt cgaagaccat
     6121 gacgaatcct cttgccgcgg ccgcgctggc gtccgcacgc cccttgaccc tcgtgtccgc
     6181 cgccgcggtg ctcgcgctgg ccgccgccga cgcctcggcc tgcgccacct gcggctgctc
     6241 gctgtcgacc gacgccgcca ccggctatcc ggcacggccc ggctggaaca tgagcctgca
     6301 gttcgactac atcgaccaga gccagctgcg ccacggcaac cacgcagtgt cgacgtccga
     6361 cgtcgccgcg atcaacgacg ccggcggcga ccaggaagtc gagcacgaca cggtcaaccg
     6421 ctacacgacg ctgggcatcg gctggatgcc ggacgaggac tggggcctgc gcctgctggt
     6481 gccctgggtc gatcgcggcc actcgaccta cgcatcggcc accaaccccg tcaccgacga
     6541 ccagctgagc accgcgcgca tcaaggggct gggcgacgtg cgcctgctcg tcacgtggca
     6601 gggcctgctg gaggagcaca acctcggcct gcaactgggc ctgaagctgc ccaccggacg
     6661 ctacggcggc gccagcgccg acggcagcgc catcgtcggc cgcaagccgg tggcgttcca
     6721 gcacggcccg ctgtcggcca acccctcgcc cgacaacctc gtcgacacca gcctgcagcc
     6781 gggcaccggc agcaccgaca tcatcgtcgg cgccttctgg caccagcccg tgagcaccac
     6841 cgtcgaggcc ttcgccaacg cgacgttcca ggccgcggtc gcccggcggc tgcgcggcca
     6901 gggccaggac ttccgcgtcg gcaacaccag cttcgtgacc ttcggcgcgc gctgggaagc
     6961 cacgcccacg ctgattccgc aggtgcaggt caacatctcg cacaagggcg ccgaccaggg
     7021 cgcgctggcc gacaacccgg acagcgaagg caccgtcgtc tacctcagcc ccggcatcac
     7081 cggcaccctc ggcgagcact ggcaggcgtt cggcttcgtg cagctgcccg tggccagcca
     7141 cctggtgggc taccagctgg cgccgcggtg gacggcgtcg gcgggggtga gctacgcgtt
     7201 ctgaaggacc ggggcggttc gcgccgctcg cccgcccggc acggacgaaa aaaaaagctc
     7261 ctggcgatcc aggagctttt ttcatgttcg gggtggggtg gctaatggga ctcgaaccca
     7321 cgacaaccag aatcacaatc tgggactcta ccaactgagc tatagccacc accggaacca
     7381 atgattatag cgcgcttttt cagttcgggg cagatgctgc ttgcgacgtg actttggcga
     7441 tacgttcgtc gttgtacttg accttgtagc gcagcttctg ggattcgtag atcgcctccg
     7501 ccaccgaatc ctcgtaggcc tgggtggcca cg
//
LOCUS       DATCRO010000876         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_617497, whole genome shotgun sequence.
ACCESSION   DATCRO010000876 DATCRO010000000
VERSION     DATCRO010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617497"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..748)
                     /locus_tag="VIN75_23855"
     CDS             complement(35..748)
                     /locus_tag="VIN75_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8977268.1"
                     /translation="MKLDTRLLAETPEGIAILLRPAGVVPRGLAYGLDFFIRLIAFTI
                     AATFLSRIKGMGTGLLLVTYFGLEWIYPIVFELLPGAATPGKRAMGLLVMMDTGLPVT
                     PSASVTRNLLRVADFMPFMFAFGMASMLLRHDFKRLGDIVGGTVVVHADQVRLAGALP
                     EAQPLAPRVPLTRAQQVAILHLAGRARRLTSERLDELATLAESALPPPDYAPGGTAPA
                     PIRPGPRLVAVAQWLMGQR"
     gene            complement(774..1406)
                     /locus_tag="VIN75_23860"
     CDS             complement(774..1406)
                     /locus_tag="VIN75_23860"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8977269.1"
                     /translation="MSGAMEQNRFAPPRADVEDSVEAEAGLVEAGRGERFLAALIDGL
                     VPTVAIIAIAVAIAIPAYETYKQSHTPGIEPPPLGSGHHLTTTWAWLGGFVLLGYFIY
                     SAVLVYLYGQTFGKRVMGIRVVHMDGGRVAFSRFIFLRWLPVAVMGCIPFVGWVITSL
                     VDPLLIFRDSRRCLHDDIASTRVVTAASSADATLRGDPKYAGANLRTFSF"
     gene            complement(1554..2147)
                     /locus_tag="VIN75_23865"
     CDS             complement(1554..2147)
                     /locus_tag="VIN75_23865"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8977270.1"
                     /translation="MDDNNENSSNRFAPPRALVADPAPSDSLVLAEPGTRLLAFLIDY
                     APAIVIGVIGGIAAAIAMPALLGRQRGTGNPFDDMGAGFVVVIGILFVAFLAWGIYNI
                     VLVYRYGQTWGKKMMGIRMVRKDGSRMSFARFFWLRGVVYGLCAAIPFVGWIVRLVDK
                     LMIFREGHQCFHDVIADTIVVTADSSQHATLAGSRQY"
     gene            complement(2236..>2490)
                     /locus_tag="VIN75_23870"
     CDS             complement(2236..>2490)
                     /locus_tag="VIN75_23870"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEY8977271.1"
                     /translation="VEVDGKLYKEYYGSASDFNKGEYRHVEGKRILEPIKGRLPDTLR
                     EMQEDLQSSISYAGGRCAGDIRKVNYVILGGENAGEHLFM"
BASE COUNT          404 a          842 c          893 g          351 t
ORIGIN      
        1 gaagcgattg aaatgagcgt gggggtcgac atgcttagcg ttgccccatc agccactgcg
       61 cgaccgcgac gagccgcggg cccggccgga tcggcgccgg cgccgtcccg cccggcgcgt
      121 agtcgggcgg cggcagcgcg ctctccgcca gcgtggcgag ctcgtcgagg cgctcggagg
      181 tgagccggcg cgcgcgtccg gccaggtgca ggatcgcgac ctgctgcgcg cgcgtcagcg
      241 gcacgcgcgg cgcgagcggc tgcgcctcgg gcagcgcgcc ggccaggcgc acctggtcgg
      301 cgtggacgac gacggtgccg ccgacgatgt cgcccaggcg cttgaagtcg tgccgcagca
      361 gcatggacgc catgccgaac gcgaacatga agggcatgaa gtcggccacc cgcagcaggt
      421 tgcgggtgac ggaggccgac ggcgtgacgg gcagcccggt gtccatcatc accagcagcc
      481 ccatcgcgcg cttgccgggc gtcgcggcgc cgggcagcag ctcgaacacg atcgggtaga
      541 tccattcgag gccgaagtag gtcaccagca gcaggccggt gcccatgccc ttgatccggg
      601 acaggaacgt cgccgcgatc gtgaacgcga tgaggcggat gaagaagtcc aggccgtagg
      661 cgagcccgcg cggcacgacg cccgccgggc gcagcaggat cgcgatgccc tcgggtgtct
      721 cggcgaggag cctggtgtcg agcttcaagg aacggaccgg cgcgggatgc ggttcagaag
      781 ctgaacgtgc gcaggttcgc gccggcgtac ttcgggtcgc cgcgcagcgt ggcgtccgcc
      841 gatgacgcgg cggtgacgac gcgcgtgctg gcgatgtcgt cgtgcaggca ccggcgcgag
      901 tcgcggaaga tcagcagcgg atcgaccagg ctcgtgatga cccagcccac gaacgggatg
      961 cagcccatca cggccaccgg cagccagcgc aggaagatga agcggctgaa cgcgacgcgg
     1021 ccgccgtcca tgtgcaccac ccggatgccc atcacgcgct tgccgaaggt ctggccgtac
     1081 aggtagacga gcaccgcgct gtagatgaag tagcccagca gcacgaagcc gcccagccag
     1141 gcccaggtgg tcgtcaagtg gtggccgctg cccagcggcg gcggctcgat gccgggcgtg
     1201 tgggactgct tgtaggtctc gtaggccgga atcgcgatgg ccaccgcgat ggcgatgatc
     1261 gcgaccgtcg gcaccaggcc gtcgatcagc gcggccagga agcgctcgcc gcgcccggcc
     1321 tcgaccaggc cggcctccgc ctcgaccgaa tcctcgacat ccgcgcgcgg cggcgcgaag
     1381 cggttctgtt ccatggcgcc ggacatcgtc cgttctccct ctttccaact cccggatacg
     1441 cggccacgtc gtcgcgcggc ctgcgtgctg cgcgcatcct accgcgcggc catgcgcaaa
     1501 cagcccggca tccctccgaa gagggagtga ccgatgtcac gccgcggggc ggatcagtat
     1561 tggcgcgagc cggcgagggt ggcgtgctgc gacgagtcgg ccgtgacgac gatggtgtcg
     1621 gcgatgacgt cgtggaagca ctggtggccc tcgcggaaga tcatcagctt gtcgaccagg
     1681 cgcacgatcc agcccacgaa cgggatggcg gcgcacaggc cgtacaccac gccgcgcagc
     1741 cagaagaagc gggcgaacga catgcggctg ccgtccttgc gcaccatgcg gatgcccatc
     1801 atcttcttgc cccaggtctg gccgtagcgg tagacgagga cgatgttgta gatgccccag
     1861 gcgaggaagg cgacgaacag gatgccgatg accacgacga agccggcgcc catgtcgtcg
     1921 aacggattgc cggtgccgcg ctgcctgccc agcagtgccg gcatggcaat ggcggccgcg
     1981 atgccgccga tcacgccgat cacgatggcc ggcgcgtagt cgatcaggaa cgccagcagc
     2041 cgcgtgccgg gctcggccag caccaggctg tccgaaggcg ccggatcggc gaccagggcg
     2101 cgcggcggcg cgaagcggtt cgaagaattc tcgttgttgt cgtccatggc cggttcctcc
     2161 ccttggctgt ccagttgacg cccggaatgc tagcgcaggc ggccgccgcg gtgcctcggt
     2221 atcggcccgg acgcctcaca tgaacaggtg ttcacccgcg ttctcgccgc ccaggatcac
     2281 gtagttgacc ttgcggatgt ccccagcgca gcggccgccg gcgtacgaga tggagctctg
     2341 gaggtcctcc tgcatctcgc gcagcgtgtc cggcagccga cccttgatcg gctcgaggat
     2401 gcgcttgccc tcgacgtggc ggtactcgcc cttgttgaag tcggacgcgc tgccgtagta
     2461 ctccttgtac agcttgccgt cgacctcgac
//
LOCUS       DATCRO010000877         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_10605, whole genome shotgun sequence.
ACCESSION   DATCRO010000877 DATCRO010000000
VERSION     DATCRO010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10605"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1258
                     /locus_tag="VIN75_23875"
     CDS             53..1258
                     /locus_tag="VIN75_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977272.1"
                     /translation="MKTTRLLAALLLAAGLAQVQVAHAVSAAVAKPLKEASDLVRAGK
                     AKEGLAKLNGVTGTNAEDNYMLARIRGAAYQSMHADGEAAQQYEAAFATGHVPPAEAG
                     RLATTVAGIYAQQHNNAKAMQWVEKAKAAGYSGSDLRQIQDYVQGNSGDYGQIARDNA
                     AKVQAAEAAGRRPEEDDLLRLADAYRHTNNKVGDLGVKEKLVLNYPSNKQYSSIYLAD
                     LPGKPGFSSRFSLDLLRLRLASGNLTAAADYMEMGQLLLQAQLPAEAKMVVDKGYAAG
                     VLGTGAEAARQQRLRDLVNKTAADQAASLAKRTADAKAGKTGDDLVAIGTEYASMGKY
                     DEGIALIQEGIAKDQLKHPEDAKLRLGVAQLQSGKGKAAAAKTLRTVQGTDGAPEIAR
                     LYLALGVAS"
     gene            1287..2270
                     /locus_tag="VIN75_23880"
     CDS             1287..2270
                     /locus_tag="VIN75_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977273.1"
                     /translation="MSSALPSFLEPLGDGLWAIDTGFHRDRFDAAYLVADGGRAAFVD
                     TGTAFAVPHLLAALDALGIRRAAVDFVIATHVHLDHAGGVGQLMQALPAATLVVHPRG
                     ARHMIDPVALWQGALAVYGAEQMARAYGELVPVPEERVRATADGETIHLAARPLVFAH
                     TPGHALHHHCIWDARSEGWFTGDNFGMAYPEFNVDGRAFIFPTTTPVQFDPDAMRASI
                     ARLLAASPRQMFLTHYSRIRHVPDCAERLLAMVDATVAAALAHRDASDRHAAIGAALM
                     RLYADSARDFGVALPDARIAELLRDDVALNAAGLIAWLDRPTRAASARAPA"
     gene            2386..3174
                     /locus_tag="VIN75_23885"
     CDS             2386..3174
                     /locus_tag="VIN75_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8977274.1"
                     /translation="MSTSISFGLEGRVVVITGASNGIGAACARRLARDGAHVALWDIA
                     EGPGNALAAELKAAGQRAAFFRCDVASKAAVDAATAATVAEFGRIDGLVNNAGIFRAA
                     EFLDVTEEDWDAVINVNLKGAFLVGQAVARHMVAQKRGAIVNMSSVNGLMAIATIASY
                     NASKGGINQLTRAMSLGLIDHGVRVNAVAPGTIATDLARDAVLTSDAARQRILGRTPM
                     KRLGEPEEIADVVGFLLSDASSYVTGEIVYVDGGRLALNYTVPA"
     gene            3177..3836
                     /locus_tag="VIN75_23890"
     CDS             3177..3836
                     /locus_tag="VIN75_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY8977275.1"
                     /translation="MYQPAHFVEKDADALLALMKAHPLAALVRGGAELSADVMPVEVE
                     RDGERWRMTAHVARANPLWREADGQPVLLLFQGPQAYVSPNWYPSKFQHGKAVPTWNY
                     SMVQAHGTLRAIQDPEWLRSFVTRLTERHETGRAVPWHVADAPADYLDAMLKAIVGIE
                     IEVTRVEGKFKLSQNRDAADRTGVVLGLEADATAGRQAQADATAQAMQAAEARRREAG
                     A"
     gene            complement(4242..4868)
                     /gene="eda"
                     /locus_tag="VIN75_23895"
     CDS             complement(4242..4868)
                     /gene="eda"
                     /locus_tag="VIN75_23895"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY8977276.1"
                     /translation="MNPLELAGYGPVIPVIVIDRLEDAVPMAKALVAGGVRVLEVTLR
                     TAAGLPAIQAIAREVPGAVVGAGTVRDADDVHAAHQAGAKFIVSPGYTKAVGDACKSL
                     GVALLPGVSTGSEIMMAADDGFTFLKLFPATAVGGIPLLKAFSGPFADIAFCPTGGVT
                     VETAPQFLALPNVKVVGGSWLTPADAMKSGDWARITELARQAAAIPRP"
     gene            complement(4998..>5213)
                     /locus_tag="VIN75_23900"
     CDS             complement(4998..>5213)
                     /locus_tag="VIN75_23900"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8977277.1"
                     /translation="AKVRDGDLIRLDAVAGTLQVLVPEAEWAARPLAERSQALGEAHG
                     AGFGRNLFAGLRRNVSTAEEGACTWLP"
BASE COUNT          793 a         1963 c         1797 g          660 t
ORIGIN      
        1 cgcagtgcgc cttctcccat tcagagtgtc accttacagg ttcgaacaac ccatgaagac
       61 cacccgtctc ctggccgccc tgctgctggc cgccggcctg gcgcaagtcc aggtcgccca
      121 cgccgtgtcc gccgccgtcg ccaagccgct caaggaagcc agcgacctgg tgcgcgcggg
      181 gaaggccaag gaaggcctgg ccaagctcaa cggcgtcacc ggcaccaacg ccgaggacaa
      241 ctacatgctg gcgcgcatcc gtggcgcggc ctaccagagc atgcacgccg acggcgaggc
      301 cgcccagcag tacgaggccg cgttcgccac cggccacgtg ccgccggccg aggcgggccg
      361 cctggcgacg acggtcgccg gcatctacgc ccagcagcac aacaacgcca aggcgatgca
      421 gtgggtcgag aaggccaagg ccgccggcta ttcgggttcc gacctgcgcc agatccagga
      481 ctacgtccag ggcaacagcg gcgactacgg ccagatcgcg cgcgacaacg ccgccaaggt
      541 gcaggccgcc gaggccgccg gccgccgtcc ggaggaggac gacctgctgc gcctggccga
      601 cgcctaccgc cacaccaaca acaaggtggg cgacctgggc gtcaaggaaa agctggtgct
      661 caactacccg agcaacaagc agtactccag catctacctg gccgacctgc cgggcaagcc
      721 gggcttctcg tcgcgtttct cgctggacct gctgcgcctg cgcctggcct cgggcaacct
      781 gacggccgcc gccgactaca tggagatggg ccagctgctg ctgcaggccc agctgccggc
      841 ggaagccaag atggtcgtcg acaagggcta cgccgccggc gtgctcggca ccggcgccga
      901 agccgcgcgc cagcagcgcc tgcgcgacct ggtcaacaag acggccgccg accaggcggc
      961 ctcgctggcc aagcgcaccg ccgacgccaa ggccggcaag accggcgacg acctcgtggc
     1021 catcggcacc gagtacgcgt cgatgggcaa gtacgacgaa ggcatcgcgc tgatccagga
     1081 aggcatcgcc aaggaccaac tgaagcaccc cgaagacgcc aagctgcgcc tgggcgtggc
     1141 ccagctgcag tccggcaagg gcaaggccgc cgcggccaag acgctgcgca cggtgcaggg
     1201 caccgacggc gcgccggaga tcgcccgcct gtacctggcg ctcggcgtcg cttcctgacc
     1261 gcctggcggc acgcggggcg acgcccatgt cctccgcgct gccgagcttc ctcgaaccgc
     1321 tgggcgacgg cctctgggcc atcgacacgg gctttcatcg cgaccgcttc gacgccgcct
     1381 acctggtggc cgacggcggt cgcgccgctt tcgtcgacac cggcaccgcg ttcgccgtgc
     1441 cgcacctgct cgccgcgctc gacgcgctgg gcatccggcg cgcggccgtg gacttcgtga
     1501 tcgccacgca tgtccacctg gatcacgccg gcggcgtggg ccagctgatg caggcgctgc
     1561 ccgccgcgac gctcgtcgtg caccctcgcg gcgcgcgcca catgatcgac cccgtcgccc
     1621 tgtggcaggg ggccctggcc gtctacggcg ccgagcagat ggcacgcgcc tacggggagc
     1681 tcgtgcccgt gccggaggaa cgcgtgcgcg ccaccgccga cggggagacg atccacctcg
     1741 ccgcccggcc gctcgtgttc gcgcacacgc cgggccacgc gctgcaccac cactgcatct
     1801 gggacgcccg cagcgaaggc tggttcaccg gcgacaactt cggcatggcc tatcccgagt
     1861 tcaacgtcga cggccgggcc ttcatcttcc cgacgaccac cccggtgcag ttcgaccccg
     1921 acgcgatgcg cgcgtcgatc gcgcgcctgc tggcggcgtc gccgcggcag atgttcctca
     1981 cccactacag ccgcatccgc cacgtgcccg actgcgccga acggctgctg gcgatggtcg
     2041 acgcgaccgt cgcagccgcc ctcgcccatc gcgacgcgtc cgaccgccac gccgccatcg
     2101 gcgcggcgct gatgcggctg tacgcggaca gcgcgcgcga cttcggcgtc gcgctgcccg
     2161 atgcgcgcat cgccgaactg ctgcgcgacg acgtggcgct caacgccgcc gggctgatcg
     2221 cgtggctcga tcggcccacg cgcgccgcgt ccgcgcgcgc gccggcctga gcgccgccgt
     2281 cggcgcgcgg cgtccgcgtt ccgcgccgta tagtggtggc atcccaagcc gcctcgcgcg
     2341 caccggcgcg acgcgcatcc tcccccgatt ccccgagatc ccgccatgag caccagcatt
     2401 tccttcggcc tggaaggccg cgtcgtcgtc atcaccggcg cgtccaacgg catcggcgcc
     2461 gcgtgcgcgc gccggctcgc gcgcgacggc gcgcacgtgg cgctgtggga catcgccgaa
     2521 ggcccgggca acgcgctggc cgccgaactg aaggccgccg gccagcgtgc cgcgttcttc
     2581 cgctgcgacg tcgcgtccaa ggccgccgtc gatgccgcca ccgccgccac cgtcgccgaa
     2641 ttcggccgca tcgacggcct cgtcaacaac gccggcatct tccgcgccgc cgagttcctc
     2701 gacgtcaccg aggaggactg ggacgcggtc atcaacgtca acctcaaggg cgcgttcctg
     2761 gtcggccagg ccgtcgcgcg gcacatggtg gcgcaaaagc gcggcgccat cgtcaacatg
     2821 tcgtccgtca acggcctgat ggccatcgcc accatcgcca gctacaacgc cagcaagggc
     2881 ggcatcaacc agctcacgcg ggcgatgtcc ctgggcctga ttgaccacgg cgtgcgcgtc
     2941 aacgcggtcg cgcccggcac catcgccacc gatctcgcac gcgacgcggt gctcaccagc
     3001 gacgcggcgc gccagcgcat cctgggccgc acgccgatga agcgcctggg cgagcccgag
     3061 gagatcgccg acgtggtcgg cttcctgctc agcgacgcct ccagctacgt caccggcgag
     3121 atcgtctacg tcgacggcgg ccgcctcgcc ctcaactaca ccgtccccgc ctgagcatgt
     3181 accagcccgc gcacttcgtc gaaaaggacg ccgacgccct gctcgccctg atgaaggccc
     3241 acccgctggc cgcgctggtc cgcggtggcg ccgagctgtc ggccgacgtc atgcccgtcg
     3301 aggtcgaacg tgacggcgag cgctggcgca tgacggcgca cgtcgcccgc gcgaacccgc
     3361 tctggcgcga ggccgacggc cagccggtgc tgctgctgtt ccagggcccg caggcctacg
     3421 tgtcgccgaa ctggtacccg agcaagttcc agcacggcaa ggccgtgccg acctggaact
     3481 actcgatggt gcaggcgcac ggcacgctgc gcgcgatcca ggatcccgag tggctgcgca
     3541 gcttcgtcac gcgcctgacc gaacgccacg agaccggccg cgccgtgccc tggcacgtgg
     3601 ccgacgcccc ggccgactac ctcgacgcga tgctcaaggc catcgtcggc atcgagatcg
     3661 aggtgacgcg cgtcgagggc aagttcaagc tgagccagaa ccgcgacgcg gccgatcgca
     3721 ccggcgtcgt gctggggctg gaggccgatg ccaccgcggg gcgccaggcg caggccgacg
     3781 cgacggcgca ggcgatgcag gccgcggaag cgcggcggcg cgaggccggc gcctaggcgg
     3841 acgcgacgcg gcgggctgga cgcccgcaag gagcggccaa cgacgcctgg ggcctcgcag
     3901 gcagccggcc ctgcgggtga tcctccagaa agcccgcctt cggcgttgcg accgcttgcc
     3961 gtggcgccac ccgcgccttg aaggcgggct ttccggaggt cacgcgggca cgcattgagt
     4021 gtgaacaggc cctagtgtcg tgagttggaa cgttgaacag gtctggagcg aagaagtgac
     4081 ggtgcccaat tccgtccgga acggttgcag accaggcgcg cccgcgacac gatgccgggt
     4141 ggcctcgcga gcgggcgcaa cgacggatgc ggccgttctg ggcggaattc ctcaacgaat
     4201 ttccaactca cgacacgagc aggcgcccga gcgttgcctg gtcagggtcg cggaatcgcg
     4261 gccgcctgcc tggccagctc cgtgatgcgc gcccagtcgc cgctcttcat cgcatcggcc
     4321 ggggtcagcc acgagccgcc gacgaccttc acgttgggca gcgcgaggaa ttgcggcgcg
     4381 gtctcgacgg tcacgccgcc ggtggggcag aacgcgatgt cggcgaacgg gccggagaag
     4441 gccttgagca gcgggatgcc gccgaccgcg gtggccggga acagcttgag gaaggtgaag
     4501 ccgtcgtcgg cggccatcat gatctcgctg ccggtggaca cgcccggcag cagcgcgacg
     4561 cccagcgact tgcaggcgtc gcccacggcc ttggtatagc ccgggctgac gatgaacttc
     4621 gcgccggcct ggtgcgccgc atgcacgtcg tcggcgtctc gcacggtgcc ggcgccgacc
     4681 acggcgcccg gcacctcgcg cgcgatcgcc tggatggcgg gcaggccggc cgcggtgcgc
     4741 agcgtcactt ccagcacgcg cacgccgccg gcgaccagcg ccttggccat ggggacggcg
     4801 tcctcgaggc ggtcgatgac gatgacggga atgacggggc cgtagccggc gagttcgagc
     4861 gggttcatgg tgcgtctctc caaaagttca gatgtctcgg ttggcccgcc gcgccgccgc
     4921 atggccggcg agcgctcggc cgcccggagc gagccggcct gagcccctcg ggggcggtga
     4981 gcggagcgag cccaggctca aggaagccag gtgcaggccc cctcttccgc ggtcgacacg
     5041 ttgcgccgca ggcccgcgaa gaggttgcgg ccgaaacccg ctccgtgcgc ctcgccgagc
     5101 gcctggctgc gctcggccag cgggcgcgcg gcccactcgg cctcgggcac cagcacctgc
     5161 agcgtgccgg ccacggcgtc caggcggatc aggtcgccgt cgcgcacctt ggc
//
LOCUS       DATCRO010000878         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_256994, whole genome shotgun sequence.
ACCESSION   DATCRO010000878 DATCRO010000000
VERSION     DATCRO010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256994"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VIN75_23905"
     CDS             <1..459
                     /locus_tag="VIN75_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977278.1"
                     /translation="AGKGPFKLWRAQWRDGTWSEPVLQALGDPAMQQVDPAVAPDESF
                     IVFSAKHPDTDEHERVYIAFREGAGWGPAIDLGAPVNLPGNDSNESRLAPDGRTLYFT
                     SDRQTPIHWPRTAAQAEADLARSAAWDNGSQNVWRVSLAPWLDAARGRAR"
     gene            complement(461..1360)
                     /locus_tag="VIN75_23910"
     CDS             complement(461..1360)
                     /locus_tag="VIN75_23910"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY8977279.1"
                     /translation="MSTLEFTPLPVPPSTGGESPFWHPRERALYWVDIPGHKLNRFAV
                     ASSEHTEWSFPSDVCSIAPRPDGGVLMARRDGLFHFDTFTGESQRVLAPPYDPRKLRF
                     NDGKADPQGRFWVGTIHDERLPRGSLYCLSPHSLDRIVDEVANSNGLAWSPDGATMYW
                     TDTKLSTVFAFDFDPHDGNLSRRRVFASFPPRQEGQPLETYGGRPDGGAVDSQGCYWA
                     ACFEGQRLARFAPDGTLLQSVPLPVRCPTMPCFGGDDLRTLYVTTSREKRPEAELAAQ
                     PWAGRVLQCRVEVPGLPTNFADV"
     gene            1463..2302
                     /locus_tag="VIN75_23915"
     CDS             1463..2302
                     /locus_tag="VIN75_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8977280.1"
                     /translation="MNPTSNYARVLGTELHYMEWGAGQAGTVIAWHGLARTGRDMDDI
                     AAHLSRRWRVICPDTIGRGLSQWSRDPERDYCFAHYERLAVALLDHLGLERVSWLGTS
                     MGGALGLRAAACALRGRIERLVLNDIGPEVAAAAIARIRQYAGNPPAFDTVGELEAYF
                     RTVYTPYGAMSDAQWRRLAETSTRRLPDGRVTPHYDPAMVQQFVHHPHDYDQWDAWGR
                     LDIPVLVLRGADSDLLEPQVARWMIERHPHATLITVPGCGHAPALNTAQHFAWVEDFL
                     AGR"
BASE COUNT          337 a          858 c          893 g          318 t
ORIGIN      
        1 gccggcaagg ggccgttcaa gctctggcgc gcgcaatggc gcgacggcac gtggtcggaa
       61 ccggtgctgc aggcgctcgg agatcccgcg atgcagcagg tcgaccccgc cgtggcgccc
      121 gacgagtcct tcatcgtctt cagcgccaag cacccggaca ccgacgagca cgagcgcgtc
      181 tacatcgcgt tccgcgaggg cgcgggatgg gggccggcga tcgacctcgg cgcgccggtc
      241 aacctgcccg gcaacgacag caatgagtcg cggctcgcgc ccgatggccg cacgctgtac
      301 ttcacgagcg atcgccagac gcccatccac tggccgcgca cggcggcgca ggccgaggcc
      361 gatctcgcgc gcagcgcggc gtgggacaac ggcagccaga acgtgtggcg cgtgtcgctc
      421 gcgccgtggc tggacgcggc gcgcgggcgt gcgcgctgag tcagacgtcc gcgaagttcg
      481 tcggcaggcc cggcacctcg acccggcact gcagcacccg gccggcccag ggctgggcgg
      541 cgagctcggc ctcggggcgc ttctcgcgcg aggtggtgac gtacagcgtg cgcaggtcgt
      601 cgccgccgaa gcacggcatc gtcgggcagc gcaccggcag cggcacggac tgcagcagcg
      661 tgccgtcggg cgcgaagcgc gccaggcgct ggccctcgaa gcaggccgcc cagtagcagc
      721 cctggctgtc gacggcgccg ccgtcgggcc ggccgccgta ggtctccagc ggctggccct
      781 cctgcctggg cgggaagctg gcgaacacgc ggcggcgcga caggttgccg tcgtgcgggt
      841 cgaagtcgaa cgcgaacacg gtgctgagct tggtgtccgt ccagtacatc gtggcaccgt
      901 ccgggctcca ggcgaggccg ttgctgttgg ccacctcgtc gacgatgcgg tccaggctgt
      961 gcgggctcag gcagtagagc gacccgcgcg gcaggcgctc gtcgtggatc gtgccgaccc
     1021 agaagcggcc ctgcggatcg gccttgccgt cgttgaagcg cagcttgcgc gggtcgtacg
     1081 gcggcgcgag cacgcgctgg ctctctccgg tgaaggtgtc gaagtggaac aggccgtcgc
     1141 gccgggccat cagcacgccg ccgtcgggcc gcggcgcgat gctgcacacg tcggacggga
     1201 acgaccactc ggtgtgctcc gagctcgcga ccgcgaagcg gttgagcttg tggccgggga
     1261 tgtcgaccca gtacagcgcc cgctcgcgcg gatgccagaa cggcgactcg ccgccggtgg
     1321 agggcggcac gggcagcggc gtgaactcga gcgtggacat ggggcacctg ggaggcagcg
     1381 gcatcgacag cgcatcctaa cggcgtacct cgatgaaacc gcgccgcttg cgttatcttg
     1441 cagcctgcgg aggcagcaag cgatgaaccc gacctcgaac tacgcccgcg tgctgggcac
     1501 cgagctgcac tacatggagt ggggcgccgg ccaggccggc acggtgatcg cctggcacgg
     1561 cctggcgcgc accggccgcg acatggacga catcgccgcg cacctttcgc ggcgctggcg
     1621 cgtgatctgt cccgacacga tcggccgcgg cctgtcgcag tggagccgtg accccgagcg
     1681 tgactactgc ttcgcgcact acgagcggct ggcggtcgcg ctgctcgacc acctcgggct
     1741 cgagcgcgtg tcgtggctgg gcacctcgat gggcggcgcg ctgggcctgc gcgcggccgc
     1801 gtgcgcgctg cgcgggcgca tcgagcgcct ggtgctcaac gacatcggcc ccgaggtcgc
     1861 ggcggcggcc atcgcgcgca tccgccagta cgccggcaac ccgccggcct tcgacaccgt
     1921 gggcgagctc gaggcgtact ttcgcaccgt ctacacgccg tacggcgcga tgagcgacgc
     1981 gcaatggcgg cgcctcgccg agacctcgac gcgccgcctg cccgacggcc gcgtcacgcc
     2041 gcactacgac ccggcgatgg tgcagcagtt cgtccaccac ccgcacgact acgaccagtg
     2101 ggacgcctgg ggccgcctcg acatccccgt gctggtgctg cgcggcgccg actccgacct
     2161 cctcgagccg caggtcgcgc gctggatgat cgagcgccat ccccacgcca cgctgatcac
     2221 ggtgccgggc tgcggccatg cgccggcgct gaacacggcg cagcacttcg cgtgggtcga
     2281 ggacttcctg gcggggcggt agcggggagg atccaagctc ggcgcgcctg tcgcccggtg
     2341 atggcatggg cgactgccgg ccacaagccg gctgtcatgt gcgtcggctc accggcaggc
     2401 cggctg
//
LOCUS       DATCRO010000879        14348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_954311, whole genome shotgun sequence.
ACCESSION   DATCRO010000879 DATCRO010000000
VERSION     DATCRO010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954311"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VIN75_23920"
     CDS             <1..824
                     /locus_tag="VIN75_23920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8977281.1"
                     /translation="LGQLEQAHAQLDASNQRLRGEIPALSVVPYHAIRARAYLQANRT
                     DEAMNAIERGLVMALEHDSEINVEELWRLKSAVHEAQGEPVQALEAYRRFHELHECQV
                     LQRVERMQLEARHDALTGLANRRRFDEYLAGVLPRAGVEAPVCLALIDVDYFKAINDR
                     FGHPIGDAALRWVAAQMEALSRQTDLPVRLGGDEFAFVLSSSLSDAQGVCERLRAAMA
                     TRPGELPRDVTMTLSIGIAETCAPCAEHELIQRADLALYGIKTRGRDGVGLALAS"
     gene            997..2535
                     /locus_tag="VIN75_23925"
     CDS             997..2535
                     /locus_tag="VIN75_23925"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977282.1"
                     /translation="MWKWFDDLRIGTKMLVVLATVLALTTMVGGLAVVELGHVSAQVR
                     GVASDALPRVRMLGALRATVLELRATQYAHMVSDSEDDQKRLKAHVAELASTLAATST
                     KFRAAATDADERAAFDAFAHQWNDYLKGNEKVLSLSGDFGIKAMEGDYRKLFDAMNES
                     LNTLLRLNDRSADALVQSAEATAGSTRIIICSAVALSVVVGLVLALAVARRIAGSLTA
                     ASRSAHDVARGDLTCSIPSGGADESGKLLAALRDMQGGLRELVGTVRQGVDSVATAST
                     EIASGNHDLSVRTEKQSSSLQFTVSAIQQMAGNIRHNTESARQATQLASSASEIALRG
                     GETVQGVVRTMDEISAASRKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VASEVRTLAQRSAVAAKEIKSLIQASVDRIEAGATLVQDAGSTMTEIVRAVRGVNDVI
                     GEIATAAGAQNEGIEQISVAVGDLDQMTQQNAALVEQSAAAAESLKEQGERLHEVVAR
                     FQLG"
     gene            complement(2537..5920)
                     /locus_tag="VIN75_23930"
     CDS             complement(2537..5920)
                     /locus_tag="VIN75_23930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977283.1"
                     /translation="MKLPFPQSLAAQFALVVSCLVALVGVVGATTIWSLAGSAQAIRV
                     LAEQRLARLQEAQDLLQHTVAIERMALRLSAIDSVDDLRDTHRQVVDHLSAFDHLVDS
                     LAAAHGGDDTDVLALHRASQRFRNTVNIAAQMRETVLAAASRAAAAASASKSLADLDD
                     DLRLQADALAVAARQQSDWFTRDYRGAVQQLVDESGRTQRWIIAEVVASLLLSWLIVR
                     QLLGRRLVARLREVSRSLRHGLGDDAPREVPVHGRDEIADMARAVEQFLEHRRQRRQA
                     EDALQQLNADLEERVALRTAELSTALDDRTREVAERQHAEEALRASERFLDSIVENIP
                     DTVFVKDAANLRFVRLNRAGELLLGYGRNELIGRNVEDLFPPHEAAFFTQKDRAVLES
                     RTLVDIPEEEVLTRHGMRLVHTMKIPILDERGEPAFLLGISRDITEQKKVDDELRRHR
                     EHLEDMIHERTGELRVAKEAADAASQAKSDFLANMSHEIRTPMNAILGMAYLALQSGL
                     NPEQFNYVQKVHGAAESLLGIINDILDFSKIEAGKLDIEAIPFNLGDVMDGLANLVGM
                     SAEQKGLELIFAEPPRLPSALVGDPSRLRQVLMNLGYNAVKFTERGEVVVEIAIVERR
                     AGAVTLRFEVRDTGIGMTAAEQRRLFQPFSQADASTSRRYGGTGLGLAISRQLVRLMG
                     GEIGVQSTPGKGSTFHFELGFALQSDAHEQPALPVDAAAGNRILIVDDNASAREILAA
                     MSTALGLRADTASSGEEALLAVAAADAGDAPYRAVLLDWKMPGLDGIECARRLAQRED
                     RRHPTPAVLMLTAFSRDTVRRRLAECGVSVGALLIKPVTPSTLFDVCNTVLGLEAPPP
                     TRASKREEALLAHQVRLRGAHLLLAEDNAINREIALTILKRAGVRVSVARDGREALDM
                     LAEQPFDGVLMDCQMPVLDGYAATRELRRQERWRALPVIAMTANAMVGDRDKAIAAGM
                     DDHIAKPIKVEEMFATLARWIRPAQAHAGTPAAPHAEAARDPLANVEGIDCRAGVAAM
                     MGDDVLYRHLLRMFRDRESDFAQRFRAARDNGAADAALRMAHDLKSVSGSLAVVAVHA
                     AAADLERACLAGADDAALAPLVRSVDGRLAPVIAQLRAL"
     gene            complement(5917..7038)
                     /locus_tag="VIN75_23935"
     CDS             complement(5917..7038)
                     /locus_tag="VIN75_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8977284.1"
                     /translation="MKGLLLAGALACASAAATVTSPETATPGAAAASLAEARRIVDAA
                     AQPAAAWTGPRTGPPAATGKQIAVVNEDLRNGGILSVNEGVLEAARVVGWKVKVYDSG
                     GTPEGRRKMLAEALASQPDGLVIVGGDAHALKPWLQPFADHHIPVVGWHVAARAGPVP
                     GTPVAMNVSTDPLDVARVTALAAIVQSGGQAGVVIFTDSSFELPKAKADAMAAVVRAC
                     AGCKLLEVQDIPISRNAELMPAATSALLARHGRRWTHALAINDIYFDYAAPVLTQAGV
                     PNNGLSMLSAGDGSESAFLRIRAGTFQTATVAEPLNLHGWQLVDEMNRLFAGERVTGY
                     VYPVHLVTAGNITADGGDRLHFDPANGYRDIYRRIWKRP"
     gene            complement(7313..8185)
                     /locus_tag="VIN75_23940"
     CDS             complement(7313..8185)
                     /locus_tag="VIN75_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8977285.1"
                     /translation="MPVVAVINRKGGSGKSTLATQVAGCLANRGLQVMLGDVDRQQSS
                     LAWLRRRAAQPLAKNAAIVGWAFGAKSVLRPPAGITHVVLDTPGGLCGYDLARVVMSA
                     DAILIPVGDSIFDRESALECHAELMTLPRVASGRCKVGIVGMRLDARTKPAPRMAAWA
                     TEHALPYVGSVRESQLYPRGAESGLTIFDAEAGKVQNDLAQWKPVMDWVEAAWLAAEK
                     MEATAKTAVPVSAPSIAPTAPAAASRRRTLVRAPLPVPTAPASEFRASREIVRPTPRA
                     QRFGWLFTVFRAWA"
     gene            complement(8410..9036)
                     /locus_tag="VIN75_23945"
     CDS             complement(8410..9036)
                     /locus_tag="VIN75_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HEY8977286.1"
                     /translation="MDTQLLAWLAGLSLGASLVVAVGAQNTFVLQQGLRREHVGAVVA
                     VCLGLDAILMTLGVNGVAAALGSHPALLAAMAWAGAAFLLWYAWQAARRAVSGQALAT
                     HAGGTAKALRVVVLQALACTLLNPHVYLDTLLLVGAVGAQQAPALRPAFIAGCVSASA
                     VWFIGLGLGARLLAPVFARPVAWRWLDGGVAVTMGLLGLRLAAGVLRA"
     gene            9115..10023
                     /locus_tag="VIN75_23950"
     CDS             9115..10023
                     /locus_tag="VIN75_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEY8977287.1"
                     /translation="MIDHHLIQAVAAVVREGSFEAAARALKVTPSAVSQRVKLIEERL
                     GSVLVVRGQPCTATDIGARLCRHAELMGALEAELHRDLPELAQAGLAPGPASLRIAVN
                     ADSLGTWFVAAMARFGREDPTLLELAVDNEDHTADWLRRGRVLGAVTSIASPVQGCRS
                     RRLGALRYLATASPDFVARHFADGVDAESIARAPSLKFDGKDELQALWVRRLLRRDVP
                     LAAHRVPSTHGGIDAALAGVGWVMNPECLVRGHIAEGRLVELVPGLPIDVALYWQSAR
                     LPVPSLERLGRAVAATAAGSLVQARA"
     gene            complement(10189..11541)
                     /locus_tag="VIN75_23955"
     CDS             complement(10189..11541)
                     /locus_tag="VIN75_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8977288.1"
                     /translation="MALTLLCSTFVVLLLLGVPVAFAIGLSCLAAFFAEGLPIATAVQ
                     MMVSGMNVFSFLAIPFFIFSGELMLHGGIADKIMNFARSLTGHVRGGLGLANVLASTL
                     FGGVSGSPVADTSAMGGVMIPLMKREGYGADYAVNVTTHASLAGALMPTSHNMIIYAF
                     AAQAAAGEIAGHTIKGVSIGDLMFAGLVPVFWIMVCMLVAAYWQAVRCGYPKREDGSS
                     MIRMFPGWGTVARTGIASLPGLAIIAIILACVMGGVTTATEAAAIAVAYSLLLTTVVY
                     RSITREKLWHALGKAARTTGVILLLIGVSNMLRWQMAYLEIPESISSALLGATQQPWL
                     MLLYINIIQIVLGMFLDMAAHILITTPLFLPLAIQMGVGPVQFGMMLLLNCALGLVHP
                     PVGTVQFVGCAIGEISMGQATRTAWPYYLAIWVAINLVTYVPHFSTWLPGAITGHAVF
                     "
     gene            complement(11552..12034)
                     /locus_tag="VIN75_23960"
     CDS             complement(11552..12034)
                     /locus_tag="VIN75_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8977289.1"
                     /translation="MTWLARLNARLSAVVLVLARLCLAGLGLVVLYGVVMRYVFNDEP
                     PYVEQLALLLVISVSMFGAACVAHDAGHIGLDSVVNRLPPAVRHTIARLVALSTLVFS
                     VVILYGAWEMARSTHDNLIPTLGISEAWRYLPLIIAGALIALFSIDRLLRRMAADDHE
                     "
     gene            complement(12051..13040)
                     /locus_tag="VIN75_23965"
     CDS             complement(12051..13040)
                     /locus_tag="VIN75_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8977290.1"
                     /translation="MTRRKTFPIRTLALAAAAVLACAAAHAREFRSADIHVKDYPTTL
                     AVKYMGDQLAKNTGGKDKIKVFADGALGSEKDTVEQVKIGALDMVRVNSAAFHGIVPE
                     SMIPSLPFLFRDIDHFRKTMYGPQGDKVLAAFEKAGFIGLALYESGARSFYAKKPIRT
                     VADMKGMKVRVQPSDLWVNLITQLGASPTPMPFAEVYTGLKTGLLDAAENNIPSYDEM
                     KHYESAPVFSETQHVMSPEVLVFSKKVWDTLGPDEQAAIRKAAKESVPYYQQLWTAKE
                     TAARASVLKAGAKIIPASEIDRKSFVDAEKPVWDKYMSTPEIRNVVQEIVNTK"
     gene            complement(13086..13982)
                     /locus_tag="VIN75_23970"
     CDS             complement(13086..13982)
                     /locus_tag="VIN75_23970"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY8977291.1"
                     /translation="MTHPRVERVRPGHRDRVGESPLWDPAASALWWVDIEACAIRRLR
                     PADDTLQSWTLPERVACLAPHSDGGLVAGLETCIAHVTPHADGRAGIVALANARHARE
                     GMRFNDGRCDRAGRFWVGSMVRDMGADIAAGRLARLDAGGLNELPIDAMYTPNGLAFD
                     PDGRGVWWSDSHPLSQRIWHAPLHDDGSVGRPRLVLDMRALPGRPDGAAVDVDGAYWI
                     CANDGGRILRLLPDGRIDREVALPVSKPSMCSFGGDALDTLYVTTIQPATPQGDDLLL
                     AGEVFALRPGTQGIAEMPFRPR"
     gene            complement(13979..>14348)
                     /locus_tag="VIN75_23975"
     CDS             complement(13979..>14348)
                     /locus_tag="VIN75_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8977292.1"
                     /translation="EDLAQLYFDRHGIETVSLRIGSSFARPRDRRMLHTWLSFDDLER
                     LVLASLAARNVGHSIICGVSANPHVWWDNTPAAHIGYVPRDSSAPFVDEIERATPPGD
                     PADPAVIYQGGAFVKQGPGK"
BASE COUNT         2047 a         5036 c         5247 g         2018 t
ORIGIN      
        1 cgctcggaca gctcgagcag gcccatgcgc agctcgatgc cagcaaccag cggctgcgcg
       61 gcgagattcc ggcgctgtcg gtcgtcccct accacgcgat ccgcgcccgc gcctacctgc
      121 aggcgaaccg gacggacgag gcgatgaacg ccatcgagcg cggcctggtg atggcgctcg
      181 agcacgacag cgagatcaac gtcgaggaac tgtggcgcct gaagtccgcg gtgcacgagg
      241 cgcagggcga gccggtgcag gcgctggagg cctaccggcg cttccacgag ctgcacgaat
      301 gccaggtgct gcagcgcgtg gagcgcatgc agctcgaggc gcgccatgac gcgctcaccg
      361 gcctggccaa ccggcgccgc ttcgacgaat acctggcggg cgtgctgccg cgcgcgggcg
      421 tcgaggcgcc ggtctgcctg gcgctgatcg acgtcgacta cttcaaggcg atcaacgacc
      481 gcttcggcca cccgatcggc gacgccgcgc tgcgctgggt cgccgcgcag atggaggcgc
      541 tgagccggca gaccgacctg ccggtgcgcc tcggcggcga cgagttcgct ttcgtgctgt
      601 cgtcctcgtt gtccgacgcc cagggcgtgt gcgaacgcct gcgcgcggcg atggccacgc
      661 gtcccggcga gctgccgcgc gacgtgacga tgacgttgag catcggcatc gccgagacct
      721 gcgcgccatg cgcggagcac gagctgatcc agcgcgccga cctggcgctc tacggcatca
      781 agacccgggg ccgggacggc gtgggcctgg cgctggcttc ctgagcgcgc gctgtcccgc
      841 cggggccaac ccgccgcggg ccgtgaattc cttggccggc gcggccgccg gaaccgtcaa
      901 gtcgcggctg gcgcgtgccg atatctcctg cgatgaggcg cgattcctgc gcccccacga
      961 cagcgcggcg tacgcgcatc cggcctggag atgtccatgt ggaaatggtt cgacgatctg
     1021 cggatcggca cgaagatgct ggtcgtcctg gcgacggtgc tcgccctgac gacgatggtc
     1081 ggcggtctcg ccgtggtcga gctggggcac gtcagcgccc aggtgcgcgg cgtcgcctcc
     1141 gacgcgttgc cgcgcgtgcg catgctgggc gcgctgcggg cgaccgtgct ggagctgcgc
     1201 gccacgcagt acgcccacat ggtgtccgat tccgaggatg accagaagcg gctcaaggcc
     1261 catgtcgccg agctcgcgtc gacgctggcg gcgaccagca cgaagtttcg tgccgcagcc
     1321 acggacgccg atgaacgcgc ggcgttcgac gccttcgccc accagtggaa cgactacctc
     1381 aagggcaacg agaaggtgct ctcgctgtcg ggcgacttcg gcatcaaggc gatggagggc
     1441 gactaccgca agctgttcga cgcgatgaac gagagcctga acacgctgct caggctcaac
     1501 gaccgcagcg ccgacgcgct ggtgcagtcg gccgaggcca cggccggcag cacgcgcatc
     1561 atcatctgct cggccgtggc gctcagcgtg gtcgtcggcc tcgtgctggc cctggcggtc
     1621 gcgcgccgca tcgccggctc gctgacggcg gcctcccgca gcgcccacga cgtggcgcgc
     1681 ggcgacctca cctgcagcat ccccagcggc ggcgccgacg agtccggcaa gctcctggcc
     1741 gcgctgcggg acatgcaggg cggcctgcgc gaactggtgg gcaccgtgcg gcagggcgtc
     1801 gattcggtgg ccaccgcctc gaccgagatc gcgagcggca accacgacct gtcggtgcgc
     1861 accgagaagc agtccagcag cctgcagttc accgtgtcgg cgatccagca gatggccggc
     1921 aacatccgcc acaacaccga gtcggcgcgc caggcgactc agctggccag cagcgcgtcg
     1981 gagatcgccc tgcgcggcgg cgagacggtg cagggcgtgg tgcgcacgat ggacgagatc
     2041 agcgccgcat cgcgcaagat cgtcgacatc atcgccgtca tcgacggcat cgccttccag
     2101 accaacatcc tcgcgctcaa cgccgccgtc gaggcggcgc gcgcgggcga gcaggggcgc
     2161 ggcttcgcgg tcgtcgcctc ggaggtgcgc acgctggccc agcgcagcgc ggttgcggcc
     2221 aaggagatca agtcgctgat ccaggcctcg gtcgaccgca tcgaggccgg cgccacgctc
     2281 gtccaggacg cgggctcgac catgaccgag atcgtccgcg ccgtgcgcgg cgtcaacgac
     2341 gtgatcggcg agatcgcgac cgccgccggc gcgcagaacg aaggcatcga gcagatcagc
     2401 gtcgcggtcg gcgacctcga ccagatgacg cagcagaacg cggcgctcgt cgaacagagc
     2461 gccgcggcag ccgagagcct caaggagcag ggcgagcgcc tgcacgaggt cgtggcgcgc
     2521 ttccagctcg gttgagtcac aaggcgcgca gctgcgcgat gaccggcgcc agccggccgt
     2581 cgacgctgcg caccagcggc gcgagggcgg cgtcgtcggc cccggccagg cacgcgcgct
     2641 cgaggtcggc ggcggccgcg tgcacggcga cgacggccag cgagccgctg acgctcttga
     2701 ggtcgtgcgc catgcgcagc gcggcgtcgg cggcgccgtt gtcacgcgcg gcccggaagc
     2761 gctgcgcgaa gtcgctctcg cggtcgcgga acatgcgcag caggtggcgg tacagcacgt
     2821 cgtcgcccat catcgcggcg acgccggcgc ggcagtcgat gccctcgacg ttcgccagcg
     2881 gatcgcgcgc cgcctcggcg tggggcgcgg ccggcgtgcc ggcgtgggcc tgcgcgggac
     2941 ggatccagcg cgccagcgtg gcgaacatct cctcgacctt gatcggcttg gcgatgtggt
     3001 cgtccatgcc ggcggcgatc gccttgtcgc ggtcgcccac catcgcgttg gcggtcatcg
     3061 cgatcaccgg cagtgcgcgc cagcgctcct ggcggcgcag ctcgcgcgtg gcggcgtagc
     3121 cgtccagcac gggcatctgg cagtccatca gcacgccgtc gaagggctgc tcggcgagca
     3181 tgtcgagcgc ctcccggccg tcgcgcgcga cgctcacgcg cacgccggcg cgcttgagga
     3241 tcgtcagcgc gatctcgcgg ttgatggcgt tgtcctcggc cagcagcagg tgggcgccgc
     3301 gcagccgcac ctggtgcgcg agcagcgcct cctcgcgctt gctggcgcgc gtgggcggcg
     3361 gcgcctcgag cccgagcacg gtgttgcaga cgtcgaacag cgtcgacggc gtcaccggct
     3421 tgatcagcaa ggcccccacc gacacgccgc attcggccag ccgccggcgc acggtgtcgc
     3481 ggctgaaggc ggtgagcatc agcaccgccg gcgtcggatg gcgacggtcc tcgcgctgcg
     3541 caagccgccg ggcgcattcg atgccgtcca gccccggcat cttccagtcg agcagcacgg
     3601 cgcggtacgg cgcgtcgccg gcgtcggcgg cggccacggc gagcaacgcc tcctcgccgc
     3661 tcgacgcggt gtcggcccgc aggcccagcg cggtactcat cgcggccagg atctcgcggg
     3721 cgctggcgtt gtcgtcgacg atcaggatgc ggttgccggc ggccgcgtcg acgggcagcg
     3781 cgggctgctc gtgggcgtcc gactgcagcg cgaagcccag ctcgaagtgg aaggtgctgc
     3841 ccttgccggg cgtgctctgc accccgatct cgccacccat caggcgcacc aactggcggc
     3901 tgatcgccag tcccagcccg gtgccgccgt agcgccggct ggtggaggcg tcggcctgcg
     3961 agaacggctg gaacaggcgg cgctgttcgg cggccgtcat gccgatgccg gtgtcgcgca
     4021 cctcgaagcg cagcgtgacc gcgccggccc ggcgctcgac gatcgcgatc tccaccacga
     4081 cctcgccgcg ttcggtgaac ttgactgcgt tgtagccgag gttcatcagc acctggcgca
     4141 gccgcgaggg gtcgcccacc agcgccgacg gcaggcgcgg cggctcggcg aagatcagct
     4201 ccaggccctt ctgctcggcg ctcatgccca cgaggttggc caggccgtcc atcacgtcgc
     4261 cgaggttgaa cgggatggcc tcgatgtcga gcttgccggc ctcgatcttc gagaagtcga
     4321 ggatgtcgtt gatgatgccc agcaacgact cggcggcgcc gtgcaccttc tgcacgtagt
     4381 tgaactgctc cgggttcagt ccgctctgca gcgcgaggta ggccatgccc aggatcgcgt
     4441 tcatcggcgt gcggatctcg tggctcatgt tggccaggaa gtcgctcttg gcctggctgg
     4501 ccgcgtcggc cgcctccttg gcgacacgca gctcgccggt gcgctcgtgg atcatgtcct
     4561 cgaggtgctc gcggtgccgg cgcagctcgt cgtcgacctt cttctgctcg gtgatgtcgc
     4621 gcgagatgcc cagcaggaat gccggctcgc cgcgctcgtc gaggatgggg atcttcatcg
     4681 tgtgcacgag gcgcatgccg tgccgcgtca gcacctcctc ctcggggatg tcgaccagcg
     4741 tgcgcgactc gagcaccgcg cgatccttct gcgtgaagaa ggcggcctcg tgcggcggaa
     4801 acaggtcctc cacgttgcgg ccgatcagct cgttgcgccc gtagcccagc agcagctcgc
     4861 cggcgcggtt caagcggacg aaacgcaggt tggccgcatc cttgacgaag accgtgtcgg
     4921 ggatgttctc gacgatgctg tcgaggaagc gctcgctggc gcgcagcgcc tcctcggcgt
     4981 gctggcgttc ggcgacctcg cgcgtgcggt cgtccagcgc cgtgctgagt tcggccgtgc
     5041 gcagtgcgac gcgctcctcg aggtcggcgt tgagctgctg cagcgcgtcc tcggcctggc
     5101 ggcgctggcg gcgatgctcg aggaactgct cgacggcgcg cgccatgtcg gcgatctcgt
     5161 cgcgcccgtg caccggcacc tcgcgcggcg cgtcgtcgcc caggccatgg cgcagcgagc
     5221 ggctcacctc gcgcagtcgc gccaccagcc ggcgtccgag cagctggcgc acgatcagcc
     5281 acgacagcag caggctggcc accacctccg cgatgatcca gcgctgcgtg cggcccgact
     5341 cgtcgacgag ctgctgcacc gcgccgcggt agtcgcgcgt gaaccagtcc gactgctggc
     5401 gcgcggccac ggccagcgcg tcggcctgca gccgcaggtc gtcgtcgagg tcggcgagcg
     5461 acttgctcgc gctcgcggcc gccgcggccc ggctcgccgc ggccagcacc gtctcgcgca
     5521 tctgcgcggc gatgttgacg gtgttgcgga agcgctggct ggcgcggtgc agggccagca
     5581 cgtcggtgtc gtcgccgccg tgcgccgcgg ccaggctgtc gacgaggtgg tcgaacgccg
     5641 acaggtggtc gacgacctgg cggtgggtgt cgcgcaggtc gtccacgctg tcgatcgccg
     5701 acaaccgcag cgccatgcgc tcgatggcca cggtgtgctg cagcaggtcc tgcgcctcct
     5761 gcagccgcgc gagccgctgt tcggccagca cgcggatggc ctgcgccgag ccggccagcg
     5821 accagatcgt cgtcgccccc accacgccca cgagcgccac gaggcacgac acgaccagcg
     5881 cgaactgcgc cgccagagac tgcgggaagg gcagtttcat ggacgcttcc agatgcggcg
     5941 gtagatgtcg cggtagccgt tggcggggtc gaagtgcagg cggtcgccgc cgtcggccgt
     6001 gatgttgccg gccgtcacca ggtgcaccgg gtacacatag cccgtgacgc gctcgccggc
     6061 gaacagccgg ttcatctcgt cgaccagctg ccagccgtgc aggttcagcg gctcggcgac
     6121 cgtggccgtc tggaacgtgc cggcgcggat gcgcaggaag gcggactcgc tgccgtcgcc
     6181 ggccgacagc atgctcagcc cgttgttggg cacgccggcc tgcgtcagca ccggggcggc
     6241 gtagtcgaag tagatgtcat tgatcgccag cgcgtgcgtc cagcgccggc cgtggcgcgc
     6301 cagcagcgcg ctggtggcag ccggcatcag ctccgcgttg cgcgagatgg ggatgtcctg
     6361 cacctccagc agcttgcagc cggcgcaggc ccgcaccacg gcggccatcg cgtcggcctt
     6421 ggccttgggc agctcgaagc tcgagtcggt gaagatgacg acgccggcct ggccgcccga
     6481 ttgcacgatc gccgccagcg cggtgacgcg ggcgacgtcc agcggatcgg tcgacacgtt
     6541 catggccacc ggcgtgccgg gcaccggccc cgccctggcg gccacgtgcc agcccacgac
     6601 cgggatgtgg tggtccgcga agggctgcag ccagggcttg agagcgtgcg cgtcgccgcc
     6661 cacgatgacc aggccgtcgg gttggctggc cagggcctcg gccagcatct tgcggcggcc
     6721 ctcgggcgtg ccgccggagt cgtacacctt gaccttccag ccgacgaccc gggccgcttc
     6781 cagcacgcct tcgttgacgc tcaggatgcc gccgttgcgc aggtcctcgt tgacgaccgc
     6841 gatctgcttg ccggtcgccg ccggcggccc ggtgcgcggg cccgtccatg ccgccgccgg
     6901 ctgcgccgcg gcgtcgacga tgcggcgcgc ctcggccagc gacgccgcgg ccgcgcccgg
     6961 agtcgccgtc tcgggcgacg tcaccgtcgc ggccgccgat gcgcaggcca aggcccctgc
     7021 caggagcaag cccttcatgc tggtctcccc cggcccctgt tcgaggccga ttgcccccgt
     7081 gtgcggacgt tcgcgaacgc cgcccgcagc gtctccctga ttgccaaata ctagcgcaac
     7141 ggcacgggcg attgggcggg ttcggcgcac cgggcgccgt gtcattcggg gttgacggcg
     7201 ggatgcgggc cgaggtgcgg tccgcgtcag cgggcgccgc catgctccgg cgggagccgg
     7261 cggaccgggc agccaagggc ggccggcgcg cggccgggtg cgacgggccc ggtcaggccc
     7321 aggcgcggaa caccgtgaac agccagccga aacgctgggc gcgcggcgtg gggcggacga
     7381 tctcgcggct ggcgcggaac tccgaggcgg gcgcggtggg caccggcagc ggcgcgcgca
     7441 cgagggtgcg gcggcgcgag gcggcggcgg gggcggtcgg ggcgatcgac ggcgccgaca
     7501 ccggcaccgc cgtcttggcc gtggcctcca tcttctcggc ggcgagccag gcggcctcga
     7561 cccagtccat gacgggcttc cactgggcca ggtcgttctg caccttgccg gcttcggcgt
     7621 cgaagatggt caggccgctc tcggcgccgc gcgggtacag ctgcgactcg cgcaccgagc
     7681 cgacgtaggg cagggcgtgc tcggtggccc aggcggccat gcgcggcgcg ggcttggtgc
     7741 gggcgtccag gcgcatgccg acgatgccca ccttgcagcg gccgctggcc acgcgcggga
     7801 gcgtcatcag ttcggcatgg cactcgagcg ccgattcgcg atcgaagatg gagtcgccga
     7861 ccgggatcag gatggcgtcg gcgctcatca cgacgcgggc gaggtcgtag ccgcacaggc
     7921 cgcccggggt gtcgagcacc acgtgcgtga tgcccgcggg cggtcgcagg acgctcttgg
     7981 cgccgaaggc ccagccgacg atggcggcgt tcttggccag cggctgggcg gcgcggcggc
     8041 gcagccaggc gagcgaggat tgctgacggt cgacgtcgcc cagcatcacc tgcaggccgc
     8101 ggttggcgag acaacccgcc acctgcgtcg ccagtgtgct cttgccgctt ccgcccttgc
     8161 gattgatgac tgctacaaca ggcatgactt cccaggcgtc gtgcgccttc gcgtgtggaa
     8221 ccgtcgcatt acggccgagt tgccgcatgt gcccgcgcat tgtgaggctg tcaacccctc
     8281 gatgctgtca gtgaacacac tcggtggtaa cgtttcgtta gccgaaaccg gcctttggac
     8341 aggccggtga caggtttgtc ggcccagcgc gggcgatctt gagccgcgcg ccccggcgcg
     8401 gcccgcccgt caggcccgca gcacgccggc cgccaggcgc aggcccagca ggcccatcgt
     8461 gacggcgacc ccgccgtcca gccagcgcca ggcgaccggg cgcgcgaaca cgggcgcgag
     8521 caggcgcgcg cccaagccca ggccgatgaa ccagacggcg ctggcgctga cgcagccggc
     8581 gatgaacgcg ggccgcagcg ccggcgcctg ctgagcgccg acggcgccga ccaggagcag
     8641 ggtgtccagg tagacgtgcg gattgagcag cgtgcaggcc agcgcctgca gcaccaccac
     8701 ccgcagcgcc ttggccgtgc cgcccgcgtg cgtggccagc gcctggccgg acaccgctcg
     8761 ccgcgccgcc tgccacgcgt accagagcag gaacgccgcg ccggcccagg ccatggcggc
     8821 gagcagcgcg ggatgcgatc cgagcgccgc cgcgacgccg ttgacgccca gcgtcatcag
     8881 gatcgcgtcc agccccaggc acacggcgac gacggcgccg acatgctcgc gccgcagccc
     8941 ctgctgcagc acgaaggtgt tctgggcgcc gacggcgacg acgagcgatg cgccgagcga
     9001 gagaccggcc agccaggcga ggagttgcgt gtccatggcg ccagtgtcgg caggcgcggg
     9061 cggccgcggc aagttagcat tgctgaatcg gcatcggcat cgcttaatcg aaggatgatc
     9121 gaccaccacc tgatccaggc cgtcgcggcg gtcgtgcgcg aaggcagctt cgaggcggcc
     9181 gcgcgcgcgc tgaaggtgac gccgtcggcg gtctcgcagc gcgtcaagct gatcgaggag
     9241 cgcctcggct cggtgctggt cgtgcgcggc cagccctgca cggccaccga catcggcgcg
     9301 cgcctgtgcc gccacgccga gctgatgggc gcgctggagg ccgaactgca ccgcgacctg
     9361 cccgagctcg cgcaggccgg cctggcgccc gggccggcgt cgctgcgcat cgccgtcaac
     9421 gccgattcgc tgggcacctg gttcgtcgcg gcgatggcgc gcttcggccg ggaggatccg
     9481 acgctgctgg agctggcggt cgacaacgag gaccacaccg ccgactggct gcgccgcggc
     9541 cgcgtgctgg gcgcggtcac gtccatcgcg agccccgtgc agggctgccg cagccggcgg
     9601 ctgggcgcgc tgcgctacct cgccaccgcc agcccggact tcgtcgcgcg ccatttcgcg
     9661 gatggcgtgg atgccgagtc gatcgcgcgc gcgcccagcc tgaagttcga cggcaaggac
     9721 gagctgcagg cgctgtgggt gcggcggctg ctgcggcgcg acgtgccgct ggcggcgcac
     9781 cgcgtgccgt cgacgcacgg cggcatcgac gcggcgctcg cgggcgtcgg ctgggtcatg
     9841 aacccggaat gcctggtgcg cgggcacatc gccgagggcc ggctcgtcga actggtgccg
     9901 gggctgccca tcgacgttgc gctgtactgg cagtcggccc ggctgccggt gccgtcgctg
     9961 gaacggctgg gacgcgcggt cgcggcgacg gccgccgggt cgctggtgca ggcgcgggcg
    10021 tgacgcgtcg cgcgtgagag gccgcccgta gagagactgt catcctgcgc gcagtcgcag
    10081 gatccaccgc ggccagccgc ttcgccgcaa ggtcgtgccc ggccgccgtg gattccgcga
    10141 ccacgcttcg ctacgcgcag aatgacgggc ctggcggact cgcagcgttc agaacaccgc
    10201 atgccccgtg atcgcgccgg gcagccacgt cgagaagtgg ggcacgtagg tcaccaggtt
    10261 gatcgccacc cagatcgcca ggtagtacgg ccaggccgtg cgcgtcgcct gacccatcga
    10321 gatctcgccg atcgcgcagc ccacgaactg cacggtgccc accggcgggt gcaccaggcc
    10381 cagggcgcaa ttgagcagca gcatcatccc gaactgcacc gggccgacgc ccatctggat
    10441 ggccagcggc aggaacagcg gcgtggtgat caggatgtgc gcggccatgt ccaggaacat
    10501 gcccagcacg atctggatga tgttgatgta cagcagcatc agccagggct gttgcgtggc
    10561 gcccagcagc gccgacgaga tgctctcggg gatctcgagg taggccatct gccagcgcag
    10621 catgttggac acgccgatca gcagcaggat cacgcccgtg gtgcgcgccg ccttgcccag
    10681 cgcgtgccac agcttctcgc gcgtgatgct gcggtagacg acggtggtca gcagcagcga
    10741 gtaggccacc gcgatggccg cggcctcggt ggcggtggtc acgccgccca tcacgcaggc
    10801 caggatgatc gcgatgatgg ccaggcccgg cagcgaggcg atgccggtgc gcgcgacggt
    10861 gccccagccg gggaacatgc ggatcatcga gctgccgtcc tcgcgcttgg gatagccgca
    10921 gcgcacggcc tgccagtacg cggccaccag catgcacacc atgatccaga agacgggcac
    10981 gagccccgcg aacatcaggt cgccgatcga cacgcccttg atcgtgtgcc ccgcgatctc
    11041 gcccgcggcc gcctgcgcgg cgaacgcgta gatgatcatg ttgtgcgacg ttggcatcag
    11101 cgcgccggcc agcgacgcgt gcgtggtgac gttgacggcg tagtccgcgc cgtagccctc
    11161 gcgcttcatc agcgggatca tgacgccgcc catggccgag gtatcggcca ccggcgagcc
    11221 ggacacgccg ccgaacagcg tcgacgccag cacgttggcc agcccgaggc cgccacgcac
    11281 gtggccggtg aggctgcgcg cgaagttcat gatcttgtcg gcgatgcccc cgtgcagcat
    11341 cagctccccc gagaagatga agaacgggat cgcgaggaac gagaacacgt tcatgcccga
    11401 gaccatcatc tgcaccgcgg tggcgatggg caggccctcg gcgaagaacg cggccaggca
    11461 cgacaggccg atggcgaagg ccaccggcac gcccagcaga agcaggacga cgaacgtgga
    11521 acagagcagc gtcagggcca tgggaagcgg gtcactcgtg gtcgtcggcc gccatgcgtc
    11581 gcagcaggcg gtcgatggag aacagcgcga tcagcgcacc ggcgatgatc agcggcaggt
    11641 agcgccacgc ttcggagatg cccagcgtcg ggatcaggtt gtcgtgggtg gaccgggcca
    11701 tctcccacgc gccgtacagg atcacgacgg agaacacgag cgtggacagc gccaccagcc
    11761 gcgcgatcgt gtggcgcacg gccggcggca accggttgac gacggagtcc aggccgatgt
    11821 gaccggcgtc gtgcgcgacg caggccgcgc cgaacatcga caccgagatc accagcagca
    11881 gcgccagctg ctccacgtac ggcggctcgt cgttgaacac gtagcgcatc accacgccgt
    11941 acagcacgac caggcccagc ccggccaggc acaggcgcgc gagcaccagc acgacggcgg
    12001 acaggcgggc gttgaggcgg gcgagccagg tcatctgcaa acctcgggcg tcacttggtg
    12061 ttgacgattt cctgcaccac gttgcggatc tccggcgtcg acatgtactt gtcccacacg
    12121 ggcttttccg cgtccacgaa gctcttgcgg tcgatctcgg aggccgggat gatcttggcg
    12181 ccggccttga gcaccgaggc gcgcgcggcg gtctccttgg ccgtccacag ctgctggtag
    12241 tagggcaccg attccttggc ggccttgcgg atcgcggcct gctcgtcggg gcccagcgtg
    12301 tcccagacct tcttggaaaa caccagcacc tcgggcgaca tcacgtgctg cgtctccgag
    12361 aacaccggcg ccgactcgta gtgcttcatc tcgtcatagg acgggatgtt gttctcggcg
    12421 gcgtccagca ggcccgtctt caggcccgtg tagacctcgg cgaagggcat cggcgtgggc
    12481 gacgcgccca gctgcgtgat caggttcacc cacaggtcgg acggctgcac gcgcaccttc
    12541 atgcctttca tgtcggccac ggtgcggatc ggcttcttcg cgtagaacga gcgcgcgccg
    12601 ctctcgtaca gcgccaggcc gatgaagccg gccttctcga acgcggccag caccttgtcg
    12661 ccctgcgggc cgtacatcgt cttgcggaag tggtcgatgt cgcggaacag gaagggcagc
    12721 gacgggatca tcgactcggg cacgatgccg tggaaggccg cgctgttgac gcgcaccatg
    12781 tccagcgcgc cgatcttgac ctgttcgacg gtgtccttct cggagccgag cgcgccgtcg
    12841 gcgaagacct tgatcttgtc cttgccgccg gtgttcttcg cgagctggtc gcccatgtac
    12901 ttgacggcca gcgtggtggg gtagtccttg acgtggatgt cggccgagcg gaactcgcgc
    12961 gcgtgcgcgg ccgcgcaggc gagcacggct gccgcggcca gcgccagcgt gcggatcggg
    13021 aaggtcttgc gtcgggtcat gtctgtctcc ggttgtcgtc ttgtgtcgag gccatgccgg
    13081 cgcgttcagc gcgggcggaa aggcatctcg gcgatccctt gcgtgccggg ccgcagcgcg
    13141 aacacctcgc cggccagcaa gaggtcgtcg ccctgcggcg tcgcgggctg gatcgtggtg
    13201 acgtagagcg tgtcgagcgc gtcgccgccg aagctgcaca tcgagggctt gctgaccggc
    13261 agcgcgacct cgcggtcgat gcggccatcg ggcagcaggc gcaggatgcg accgccgtcg
    13321 ttggcgcaga tccagtaggc gccgtcgacg tcgaccgccg cgccgtcggg gcggccgggc
    13381 agggcgcgca tgtccagcac gagccgcggc ctaccgacgc tgccgtcatc gtgcagcggc
    13441 gcgtgccaga tgcgctgcga cagcgggtgg ctgtcgctcc accacacgcc gcggccgtcc
    13501 gggtcgaacg cgaggccgtt gggggtgtac atcgcgtcga tgggcagttc gttcaggccg
    13561 cccgcgtcga gccgcgccag gcggccggcg gcgatgtcgg cgcccatgtc gcgcaccatg
    13621 ctgccgaccc agaagcggcc ggcgcggtcg cagcggccat cgttgaagcg catgccctca
    13681 cgcgcgtggc gcgcgttcgc gagcgcgacg atgccggcgc ggccgtcggc gtgcggcgtg
    13741 acgtgcgcga tgcaggtctc cagccccgcg acgagcccgc cgtccgagtg cggcgcgagg
    13801 caggcgacgc gctcgggcaa ggtccatgac tgcagcgtgt cgtcggccgg gcgcagcctg
    13861 cggatcgcgc aggcctcgat gtcgacccac cacagcgcgc tggcggcggg gtcccacagc
    13921 gggctctcgc ccacgcggtc gcgatggccc gggcgcacgc gttcgacgcg gggatgggtc
    13981 acttgccggg cccctgcttc acgaacgcgc cgccctggta gatgacggcg gggtccgccg
    14041 ggtcgccggg cggcgtcgcg cgttcgatct cgtcgacgaa cggcgccgag ctgtcgcgcg
    14101 ggacgtagcc gatgtgcgcg gcgggcgtgt tgtcccacca cacgtgcggg ttggcgctga
    14161 caccgcagat gatgctgtgg ccgacgtttc ttgcggcgag cgaagcgagc acgaggcgct
    14221 ccaggtcgtc gaagctcagc cacgtgtgca gcatgcggcg gtcgcgcggc ctggcgaacg
    14281 acgagccgat gcgcaggctc accgtctcga tgccgtggcg gtcgaagtac agctgcgcga
    14341 gatcctcg
//
LOCUS       DATCRO010000880         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_280236, whole genome shotgun sequence.
ACCESSION   DATCRO010000880 DATCRO010000000
VERSION     DATCRO010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280236"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..800)
                     /locus_tag="VIN75_23980"
     CDS             complement(3..800)
                     /locus_tag="VIN75_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8977293.1"
                     /translation="MTADPHCDLGTRPAPNARKAGDRVPDVTFRLRRGADWEETSTAR
                     IFGGRNVIVFALPGAFTPTCSTVHLPRYEELAPEFEKRGIDEIVCVAVNDPFVMQQWG
                     LEEAASKVHLLPDGNAAFTRGMGMLVDKSDLNFGARSWRYSMLVRDGVIEKLFVEPDK
                     PGDPFEVSDADTMLRHLDPSATLPDEVAIFSREGCPFCARTRSALQEAGIAFTDICLP
                     PLQRSRALRAIAGAQTVPQVFVNGRLVGDSEATLQWLATRSRQGGHG"
     gene            971..2206
                     /locus_tag="VIN75_23985"
     CDS             971..2206
                     /locus_tag="VIN75_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977294.1"
                     /translation="MSSASPVLDSAVPVRATTDRERHFWPAVWALGVGSFAIGTGEFV
                     IMGLLPEVARDLGVTIPQAGHVITAYALGVVVGAPVLAVLAASWPRRALLIALMALFA
                     AGNFASALAPGYLSMNLMRLLAGLPHGTYFGVAALMAASLAPPGRRASAVGLVMSGLT
                     SATLAGVPLAAWLGQHLGWRAAFVLVGAIAVLAIVLIRRGVPDDPAPHGASPLRELGA
                     LARTQVWLTLGIAAIGFGGMFAVFSYVKPLLTEVTGLPLDGVPLVLALFGAGMVGGNL
                     VGARLADKALMPTIGGLLVWSALVLGAIPFTAPYTVAICINVFLLGTVVALGPALQIR
                     LMDTAGDAQTLAAALNHSAFNIANALGAWLGGAAIAAGLGWTSTGPVGVLLAFGGLVV
                     FAASAWTRRRMRAHGEAHA"
     gene            complement(2219..2710)
                     /locus_tag="VIN75_23990"
     CDS             complement(2219..2710)
                     /locus_tag="VIN75_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977295.1"
                     /translation="MRLAIDIAFATAVSAAVLYAGWHWYGPFGLVFGAPILAIFGRPL
                     VEILGSMPRRVRYLALRRHGGRYYEFRGRAMDIHVDADGRCWVATADVRKITALPAEP
                     VLLRLVPGQVRELGDPVAWRLSTEGAAQVLGKSTDAQVAKFLTWLEREVALPARNRRE
                     RRM"
     gene            complement(2707..3741)
                     /locus_tag="VIN75_23995"
     CDS             complement(2707..3741)
                     /locus_tag="VIN75_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEY8977296.1"
                     /translation="MSEDLVLVTGGSGFIATHCIVQLLAAGHRVRTTVRSLEREPQVR
                     AMLRSAGADAGDRLAFAAADLTGDAGWDEAAAGCDRVLHVASPFPVNVPRHEDELIVP
                     AREGALRVLRASRGAGVRRVVLTSSFAAVGYGHPEVDRAFDESDWTNLDGDGLTAYVK
                     SKTLAERAAWSFMGREGGDMELAVVNPVGVFGPALGADFSTSIEIIKRMLDGALPGLP
                     RITFGAVDVRDVADLHLRAMTHPEAAGERFLAVAGDFLSLRDMALVLRERMGDAARRV
                     PARELPDWLLRIVALVDKSVAQIVPELGKHKNATSEKARRVLGWTPRTADEAIVATAE
                     SLVRLGLVRR"
     gene            3947..>4351
                     /locus_tag="VIN75_24000"
     CDS             3947..>4351
                     /locus_tag="VIN75_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8977297.1"
                     /translation="MRLRLHVLVPAAAAVACLALAGCHRKPATETSPVPAAASGPRPA
                     ASAVVAELKDVLAAHRQIIVLFADEKRQTDVQRAAADEVGQAIFHDNRAHVARIESQF
                     ETLAAGGEDPTPALAPILDFIESDPSLFDADRL"
BASE COUNT          568 a         1643 c         1544 g          596 t
ORIGIN      
        1 ccctacccgt gcccgccttg ccggctgcgg gtcgcgagcc actgcaaggt cgcctccgaa
       61 tcgccaacca ggcgcccgtt gacgaacacc tgcggcaccg tctgcgcccc ggcgatcgcc
      121 cgcaacgcgc gcgaccgctg cagcggcggc aggcagatgt cggtgaaggc gatgcccgcc
      181 tcctgcagcg ccgacctggt gcgcgcgcag aacgggcagc cctcgcggct gaagatcgcc
      241 acctcgtcgg gcagcgtcgc cgacggatcg aggtggcgca gcatcgtgtc ggcgtcggac
      301 acctcgaacg ggtcgcccgg cttgtccggc tccacgaaca gcttctcgat gacgccgtcg
      361 cgcaccagca tcgagtagcg ccacgagcgc gcgccgaagt tcaggtcgct cttgtccacg
      421 agcatgccca tgccgcgcgt gaacgccgcg ttgccgtcgg gcagcaggtg caccttgctc
      481 gccgcctcct cgaggcccca ctgctgcatg acgaacgggt cgttgacggc gacgcagacg
      541 atctcgtcga tgccccgctt ctcgaattcc ggcgccagtt cctcgtagcg cggcaggtgc
      601 accgtcgagc acgtcggcgt gaacgcgccg ggcagcgcga acacgatcac gttgcggccg
      661 ccgaagatcc tggcggtgct tgtctcctcc cagtcggcgc cgcggcgcag cctgaacgtg
      721 acgtcgggca cccggtcgcc cgccttgcgg gcattcgggg cggggcgcgt gcccaggtcg
      781 cagtgcggat cggcggtcat ggcggggctc cctggtcgga cggcggaccg ccggagtcgg
      841 caagccacgt gccgccgcgc ggcggttcac atcgcgaacc ggcggatgcc gaccgggaag
      901 cccggcgcga gcaagccttg ctgcaatgca acacaatgat ggaaccgctt ccgcgccagc
      961 cgcccagccg atgagttccg cctcgcccgt cctcgattcc gccgtccccg tccgtgccac
     1021 gaccgaccgc gagcgccact tctggcccgc cgtgtgggcc ctcggcgtgg gcagcttcgc
     1081 gatcggcacc ggtgaattcg tgatcatggg cctgctgccc gaggtcgcgc gcgacctggg
     1141 cgtcaccatc ccgcaggccg gccacgtcat cacggcgtat gcgctgggcg tggtggtcgg
     1201 cgcgccggtg ctggccgtgc tggccgccag ctggccgcgg cgcgcgctgc tgatcgcgct
     1261 gatggcgctg ttcgcggccg gcaacttcgc cagcgcgctg gcgcccggct acctgtcgat
     1321 gaacctgatg cggctgcttg ccggcctgcc gcacggcacc tacttcggcg tcgcggcctt
     1381 gatggcagcg tcgctcgccc cgcccggccg gcgcgcgagc gcggtcggcc tcgtgatgtc
     1441 gggcctgacc agcgccaccc tcgccggcgt gccgctggcc gcgtggctgg gccagcacct
     1501 gggctggcgc gccgcgttcg tgctcgtggg cgccatcgca gtgctggcca tcgtgctgat
     1561 ccgccgcggc gtgcccgacg accccgcccc gcacggcgcg agcccgttgc gcgaactcgg
     1621 ggcgctggcg cgcacgcagg tctggctcac gctgggcatc gccgcgatcg gcttcggcgg
     1681 catgttcgcc gtcttcagct acgtcaagcc gctgctgacc gaggtcaccg ggctgccgct
     1741 ggacggcgtg ccgctggtgc tggcgctgtt cggcgcgggc atggtcggcg gcaacctggt
     1801 cggcgcgcgc ctggccgaca aggcactgat gcccaccatc ggcggactgc tcgtgtggag
     1861 cgcactggtg ctgggcgcca tcccgttcac ggcgccgtac acggtggcca tctgcatcaa
     1921 cgtcttcctg ctcggcaccg tcgtcgccct cggcccggcg ctgcagatcc ggctgatgga
     1981 caccgccggc gacgcgcaga cgctggccgc ggcgctgaac cactcggcgt tcaacatcgc
     2041 caacgcgctg ggcgcgtggc tcggcggcgc cgccatcgcg gccggcctcg gctggacgtc
     2101 gacgggcccg gtgggcgtgc tgctggcctt cggcggcctc gtggtgttcg ccgcctcggc
     2161 gtggacgcgc cggcgcatgc gcgcccacgg cgaggcgcac gcctgacgca agggcgccct
     2221 acatccggcg ctcgcgccgg ttgcgtgccg gcagcgccac ctcgcgctcc agccaggtca
     2281 ggaacttcgc gacctgggcg tccgtcgact tgcccagcac ctgcgccgcc ccttcggtcg
     2341 acaggcgcca cgcgaccgga tcgcccagtt cgcgcacctg ccccggcacc aggcgcagca
     2401 gcacgggctc ggccggcagc gcggtgatct tgcgcacgtc ggccgtggcg acccagcagc
     2461 gcccgtccgc gtcgacgtgg atgtccatgg cgcggccgcg gaactcgtag tagcgcccgc
     2521 catggcgacg caacgccagg taccgcacgc gtcgcggcat cgagcccagg atctccacca
     2581 gcggccgtcc gaagatcgcc aggatcggcg cgccgaacac caggccgaac gggccgtacc
     2641 agtgccatcc cgcgtacagc accgccgccg agaccgcagt ggcgaacgcg atgtcgatcg
     2701 ccagcctcat cgcctgacga gccccaggcg cacgaggctc tcggccgtcg cgacgatggc
     2761 ctcgtcggcc gtgcgcggcg tccagcccag cacgcggcgc gccttctcgc tggtcgcgtt
     2821 cttgtgcttg cccagctcgg gcacgatctg ggcgacggac ttgtcgacca gcgccacgat
     2881 gcgcagcagc cagtcgggca gttcgcgcgc gggcacgcgg cgcgccgcgt cgcccatgcg
     2941 ctcgcgcagc acgagcgcca tgtcgcgcag cgacaggaag tcgccggcca ccgccaggaa
     3001 gcgctcgccg gcggcctcgg gatgcgtcat cgcccgcagg tgcaggtcgg cgacgtcgcg
     3061 cacgtccacg gcgccgaagg tgatgcgcgg cagcccgggc agcgcgccgt ccagcatgcg
     3121 cttgatgatc tcgatggatg tggagaagtc ggcaccgagc gccgggccga agacgcccac
     3181 cggattgacg acggccagtt ccatgtcgcc gccctcgcgg cccatgaagc tccaggcggc
     3241 gcgctcggcg agcgtcttgg acttgacgta ggccgtcagg ccgtcgccgt ccaggttggt
     3301 ccagtcgctc tcgtcgaacg cccggtccac ctccgggtgg ccgtagccga ccgccgcgaa
     3361 cgacgacgtc agcacgacgc gcctcacgcc cgcgccgcgg gacgcgcgca gcacgcgcag
     3421 cgcgccctcg cgcgcgggca cgatcagctc gtcctcgtgc ctggggacgt tgacggggaa
     3481 cggcgaggcc acgtgaagca cgcggtcgca gccggcggcg gcctcgtccc agccggcgtc
     3541 gccggtgagg tcggcggcgg cgaacgcgag ccggtcgccg gcgtcggcgc ccgccgatcg
     3601 cagcatggcg cgcacctggg gctcgcgctc gagcgagcgc accgtggtgc gcacgcggtg
     3661 gccggcggcg agcagttgca cgatgcagtg cgtggcgatg aagcccgaac caccggtgac
     3721 gaggacgagg tcctcgctca tggccggctc ccggtcgcgg ggactgtgcg gcgttcgggc
     3781 atggcgttct ccgtgcatca gcgatgcgag atggcgagga acgcaacgat atcgcacatg
     3841 gcgcaggccg aggcgggcga ccacggacgt tgccgaccgc gcagggcgca ggaagggtca
     3901 cgtatcatcg cgaccgattc cacactcgcc gccgcgcgcc gagccgatgc gccttcgcct
     3961 ccacgtcctc gtcccggccg ccgccgccgt cgcctgcctc gccctggcgg gctgccacag
     4021 gaagcccgcg acggagacgt ccccggtccc ggccgccgcg agcgggccgc gccctgcggc
     4081 cagcgcagtc gtcgcggagc tcaaggacgt gctggccgcg caccgccaga tcatcgtgct
     4141 gttcgccgac gagaagaggc agaccgacgt gcagcgcgcg gccgccgacg aggtgggcca
     4201 ggcgatcttc cacgacaacc gtgcgcacgt cgcccggatc gaatcgcagt tcgagacgct
     4261 ggccgccggc ggggaggatc cgactcccgc gctcgcgccc atcctggact tcatcgagag
     4321 cgacccctcg ctgttcgacg ccgaccgcct c
//
LOCUS       DATCRO010000881         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_707311, whole genome shotgun sequence.
ACCESSION   DATCRO010000881 DATCRO010000000
VERSION     DATCRO010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707311"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VIN75_24005"
     CDS             <1..123
                     /locus_tag="VIN75_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8977298.1"
                     /translation="RQPVCGMGVCGECRVTVDGRAHRLGCQTACAPGMEILTDG"
     gene            116..1435
                     /locus_tag="VIN75_24010"
     CDS             116..1435
                     /locus_tag="VIN75_24010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEY8977299.1"
                     /translation="MAESDNALHAQVLVIGAGPAGLAAARAAAAAGCDVLLLDAAPQA
                     GGQIWRARQGVAAAPVADALARLSAAGARVLAGARVTMVLPDRTLLVDGPQGACRVRW
                     QRLILATGARERLLPLPGWTLPGVFGAGGLQALVKGGWPIAGRRVLVAGTGPLLLAAA
                     ATLRAERAQVVAIAEEAGTPALARFAGTLVRHPRKAWQALKLGAALRGVTMLRGTRIV
                     AIEGDGHVERVVLEGEGGRTRTIACDAVAAGWGLVPQTELAQALGCTLAPRFGAAAIA
                     VDGVQRGSVAGVFAAGECSGVAGVQAARLQGEIAGTIAAAELQGRSSDTRTLAARLAR
                     EHDFAAAVAATFPMPRDWAARLHDDTLVCRCEDVAWSAVRAQPDLRAAKLATRCGMGH
                     CQGRLCHDTLAAVLHWPRLPQRAPLAPAPLASLLALPDNPSHKEHST"
     gene            1432..>2070
                     /locus_tag="VIN75_24015"
     CDS             1432..>2070
                     /locus_tag="VIN75_24015"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8977300.1"
                     /translation="MSMTWNGVMPAITTPFTADGEVDHDFLASHALWMVEAGCTGIVA
                     LGSLGEAATLSVPEKLDVLRTLAKALGGRVPLVAGIAALSTDEACGLAQAAAEIGCAG
                     LMVLPPYVYSTDWREMRAHVAAVIQATDLPCMLYNNPIAYRTDFRPEQIEELAHAHSN
                     LVAVKESSADIRRIAAIRHRLGERLAILVGVDDIIVEGIDAGATGWVAGLVNA"
BASE COUNT          231 a          768 c          795 g          276 t
ORIGIN      
        1 cgccagccgg tgtgcggcat gggcgtgtgc ggcgagtgcc gcgtcacggt cgacggtcgc
       61 gcgcatcggc tgggctgcca gacggcctgt gccccgggca tggagatcct gaccgatggc
      121 tgagtcggac aacgcgctgc acgcccaggt gctcgtcatc ggcgccggcc ccgcgggcct
      181 ggcggcggcg cgtgcggccg cggccgccgg ctgcgacgtg ctgctgctgg acgcggcgcc
      241 gcaggccggc gggcagatct ggcgcgcgcg ccagggcgtg gccgcggcgc cggtggcgga
      301 cgcgctcgcg cgtctctcgg cggcgggcgc gcgggtgctc gccggggcgc gcgtgacgat
      361 ggtgctgccc gatcgcacct tgctggtcga cggcccgcag ggcgcgtgtc gcgtgcgttg
      421 gcagcgcttg atcctggcga ccggcgcgcg cgagcggctg ctgccattgc cgggctggac
      481 cttgcccggc gtgttcggcg cgggcggcct gcaggcgctc gtcaagggcg gctggccgat
      541 cgcggggcgt cgcgtgctgg tcgccggcac cgggccgctg ctcctcgctg cggcggccac
      601 gctgcgcgcg gagcgggcgc aggtcgtggc catcgcggaa gaggccggca cgcccgcgct
      661 cgcgcgcttc gcgggcacgc tcgtgcgcca tccgcgcaag gcctggcagg cactgaagct
      721 cggcgcggcg ctgcgcggcg tgacgatgct tcgcggcacg cgcatcgtcg cgatcgaggg
      781 cgacgggcac gtggagcgcg tcgtgctgga aggcgagggc ggtcgaacgc gcacgatcgc
      841 ctgtgatgcg gtcgccgccg gctggggcct ggtgccgcag accgaactcg cgcaggcgct
      901 cggctgcacc ctcgcgccgc gtttcggcgc cgcggcgatc gccgtggacg gcgtgcagcg
      961 cggcagcgtc gccggcgtgt tcgcggccgg cgagtgctcc ggcgtcgcag gcgtgcaggc
     1021 ggcccggctg cagggcgaga tcgccggcac catcgcggcg gcggaactgc agggccggtc
     1081 atccgacacc cgcacgctcg ccgctcgcct ggcgcgcgag catgatttcg ccgcggccgt
     1141 ggccgccacc ttcccgatgc cgcgcgactg ggccgcgcgc ctgcacgacg acacgctcgt
     1201 ctgccgctgc gaggacgtgg cgtggtcggc cgtgcgcgcg caacccgacc tgcgcgccgc
     1261 caagctcgcc acccgctgcg gcatggggca ttgccagggc cgcctgtgtc atgacacgct
     1321 ggcggccgtg ctgcactggc cccgccttcc ccaacgcgcg ccgctcgcac cggcgccgct
     1381 cgccagcctc ctggcgctgc ccgacaaccc atcccacaag gagcattcga catgagcatg
     1441 acctggaacg gcgtgatgcc cgccatcacc acccccttca ccgccgacgg cgaggtcgac
     1501 cacgacttcc tggcgtcgca cgcgctgtgg atggtcgagg ccggctgcac cggcatcgtc
     1561 gccctcggct cgctcggcga ggcggccacg ctgtccgtgc ccgagaagct ggacgtgctg
     1621 cgcacgctgg ccaaggcgct gggcggccgc gtgccgctcg tcgccggcat cgcggcgctg
     1681 tccaccgacg aggcctgcgg cctggcgcag gccgcggccg agatcggctg cgcgggcctg
     1741 atggtgctgc cgccgtacgt ctactcgacc gactggcgcg agatgcgcgc gcacgtggcc
     1801 gccgtgatcc aggcgaccga cctgccgtgc atgctctaca acaacccgat cgcgtaccgc
     1861 accgacttca ggcccgagca gatcgaggag ctcgcgcacg cgcactcgaa cctcgtcgcc
     1921 gtcaaggagt cgagcgccga catccgccgc atcgccgcga tccgccatcg cctgggcgag
     1981 cgcctggcga tcctggttgg cgtggacgac atcatcgtcg agggcatcga tgccggcgcg
     2041 acgggctggg tggcggggct ggtcaacgcg
//
LOCUS       DATCRO010000882         4140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_932364, whole genome shotgun sequence.
ACCESSION   DATCRO010000882 DATCRO010000000
VERSION     DATCRO010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932364"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VIN75_24020"
     CDS             <1..1159
                     /locus_tag="VIN75_24020"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8977301.1"
                     /translation="STCAPWNASTAYNGGATVSRNGVNYKANWWTQGDDPATHNGPAG
                     SGQPWTVTTTCSGTTPPPPTPAPPPPTPAPPPPTPTPPPPTPAPPPSNGSLPAHVLMG
                     YWQNFNNGAPVLTIAQVPTTYDVIAVAFANATGVPGQISFAIDSGVTGYSDAQFKADI
                     VTAHSRGQKVVLSVGGQNGSISVSDSTSASNFASSAASILTNYGFDGIDIDLENGINP
                     SSMASAISQLKALKPNALVTAAPQTLDTQNSQSSYFSFASMLGANMTMMNTQYYNSGS
                     MLGCDGGVYSQGTENFITALACTQLNSLPASEIGIGLPASPSAAGSGYVSPSVVVAAL
                     QCLANGSNCGSFRPPANHPAIRGVMTWSINWDAATGYQFANTVSPALRALP"
     gene            complement(1291..>4140)
                     /locus_tag="VIN75_24025"
     CDS             complement(1291..>4140)
                     /locus_tag="VIN75_24025"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEY8977302.1"
                     /translation="PPPPPPSAAPLITSQSFYAAVLQEGTATFTVAAQNATGYEWQQS
                     PDGATWTDVSGGNTATLNVVEPLSANGLQYRVIVSGPGGQVTSVPAPLSVYPVMALQP
                     MSRAAFDGDDVSFTGGATFDLAHISQQWQESRDGQTWTDIAGATSQMLTLHAFALTDS
                     GLQVRAVVSAFGHTYTSNVATLSVTTNPSDPVFVAQPEDATVAAGGDVRFSSWTSGGG
                     AMPAYQWQSSTDRGGQWNAIPGANLQVLTLSGVAAASDGELLRLQVTSNGRAFLSQAA
                     TLHVGAAGAGRLDLLAGALGGIGDLDGTGSGAWLQGPGMPGFDAQGNLFVLDNQRFKR
                     IDPSGRVTTIAGRNDSGGQSDGAGGTAMFYGFFGSGTAVAANGDAYVAEAGNCKIRKV
                     TADGTVTTFAGTYACGSADGTGSAASFNQPRGLAIDAAGNLYVADTGNDTIRRITPAG
                     VVTTLAGTAGVAGSTDGASGVALLNAPWGITVDPQGQVVSFIDGGHTVRQWTAAGGVK
                     TLAGSAGTSGAADGTGAAASFKYPTGLATDSNGTLYVADSGNDTIRKVTPAGVVTTIA
                     GLAGAQGAIDATGAAARFSKLTGVALAPDGSLAVVDGTSVRRIGTAGNVTTLAGSIEP
                     QGQADGVGPGARFDLPRSVIADANGNLIVGDGHGLQRRVARDGTTSTFNTGGYADGMA
                     FDHSGNLIVADGSFDCVVRRVSPQGSFIQVAGNSSNCQAVDGNGTQAGFSLPRGVAVG
                     LDGEIYVMDRGRLRRIATNGDVTTLTSTDPNVRCGSDGALSSVIFCGDGSMVIDEAGN
                     LFIADAQAVRRVDAVTQQVSTIAGSNSGVWGSVDGNGSDARFGWLQSITRDPTGNLYV
                     SDFIFHNVRRIDPAGNVTTLVGRAGVPGIVLGTAAALDYPEGLAMMDATHLAILSMDA
                     VLVYTLPGGTPLAATASHRGRATQAARPAGKSRGH"
BASE COUNT          587 a         1523 c         1422 g          608 t
ORIGIN      
        1 gtcgacgtgt gcgccgtgga acgcgagcac cgcctacaac ggcggcgcca ccgtcagccg
       61 caacggcgtc aactacaagg cgaactggtg gacgcagggc gacgaccccg cgacccacaa
      121 cggccccgcc ggcagcggcc agccctggac ggtgaccacg acctgcagcg gcaccacgcc
      181 gccgccacct acgccggccc cgccgccgcc cacgccggcc ccgccgccgc ccaccccgac
      241 cccgccgccg ccgacgccgg cgccgccgcc gtccaacggc agcctgccgg cgcacgtgct
      301 gatgggctac tggcagaact tcaacaacgg cgcgcccgtg ttgacgatcg cccaggtccc
      361 caccacctac gacgtcatcg cggtggcctt cgccaacgcc accggcgtgc cgggccagat
      421 cagcttcgcg atcgactccg gggtgaccgg ctacagcgac gcgcagttca aggccgacat
      481 cgtcacggcg cattcgcgtg gccagaaggt ggtgctgtcg gtgggcggcc agaacggctc
      541 gatctcggtc agcgattcga ccagcgcgtc gaacttcgcc agcagcgcgg cgtccatcct
      601 gacgaactac ggtttcgacg gcatcgacat cgacctggag aacggcatca acccgtcgtc
      661 gatggcgtcg gcgatcagcc agctcaaggc cctcaagccg aatgcgctgg tcacggcggc
      721 gccgcagacg ctggacacgc agaactcgca gagttcgtac ttctcgttcg cctcgatgct
      781 cggcgcgaac atgacgatga tgaacacgca gtactacaac tccggctcga tgctgggttg
      841 cgacggcggc gtctacagcc agggcacgga gaacttcatc accgcgctgg cctgcacgca
      901 gctgaactcg ctgcccgcct cggagatcgg catcggcctg ccggcctcgc cgagcgcggc
      961 gggcagcggc tacgtcagcc cgtcggtcgt cgtcgcggcg ctgcagtgcc tggccaacgg
     1021 cagcaactgc ggctcgttca ggccgccggc caaccatccg gccatccgcg gcgtgatgac
     1081 gtggtcgatc aactgggacg ccgccaccgg ctaccagttc gccaacacgg tgtcgccggc
     1141 gctgcgcgcg ttgccgtagg acggccggat cgctgatccg gtcctgcagg atggttgttg
     1201 ccttcgcggg caacaaccat tttttttcgc ccgcgcccgc cctgccgggg aggtggcgcg
     1261 gccgcggtca tcgcacccgc acggccctgc tcaatggccc ctggactttc ccgccggacg
     1321 cgccgcctgc gtcgcccggc cgcgatgcga cgcggtggcc gccaggggcg tcccgccggg
     1381 cagcgtatag acgagcaccg catccatcga caggatcgcc aggtgcgtcg cgtccatcat
     1441 cgccaggcct tccgggtagt cgagcgccgc cgcggtgccc agcacgatgc ccggaacgcc
     1501 ggcacgtccg accagcgtcg tcacgttgcc cgccggatcg atgcggcgca cgttgtggaa
     1561 gatgaagtcg ctgacgtaca ggttgcccgt cgggtcgcgc gtgatgctct gcagccagcc
     1621 gaaccgggcg tcgctgccgt tgccgtccac cgagccccag acgccggagt tcgatcctgc
     1681 gatcgtgctg acctgctgcg tgaccgcgtc gacgcggcgc acggcctgcg cgtcggcgat
     1741 gaacaggttg ccggcctcgt cgatgaccat gctgccgtcg ccgcagaaga tgacgctcga
     1801 caacgcgcca tcgctgccgc agcgcacgtt cgggtcggtg gaggtcagcg tcgtcacgtc
     1861 gccgttggtc gcgatccgcc gcagccggcc gcggtccatc acgtagatct cgccatcgag
     1921 cccgaccgcc acgccgcgcg gaagggagaa tccggcctgc gttccgttgc cgtcgacggc
     1981 ctggcagttc gacgaatttc cagccacctg gatgaagctg ccctgcggac tcacgcggcg
     2041 cacgacgcag tcgaagctgc cgtcggcgac gatcaggttg cccgaatggt cgaacgccat
     2101 cccgtcggcg tagccgcccg tgttgaacgt gctcgtcgtg ccgtcgcgcg cgacccgccg
     2161 ctgcaggccg tggccgtcgc ccacgatcag attgccgttg gcgtcggcga tcaccgaacg
     2221 cggcaagtcg aagcgcgcgc ccgggccgac gccgtcggcc tggccttgcg gctcgatgct
     2281 gcccgccagc gtggtgacgt tgccggcggt gccgatgcgc cgcacactgg tgccgtccac
     2341 cacggccagg gagccgtccg gcgcgagcgc gacgcctgtc agcttcgaga accgggcggc
     2401 cgctcccgtc gcgtcgatcg cgccctgggc gcccgccagg ccagcgatcg tggtgacgac
     2461 gccggccggg gtgaccttgc ggatcgtgtc gttgccgctg tcggccacgt agagcgtgcc
     2521 gttcgaatcg gtggccaggc cggtcgggta cttgaagctg gccgcggccc ccgtgccgtc
     2581 cgcggcaccg ctcgtgcccg cgctgcccgc cagcgtcttg acgcccccgg ccgccgtcca
     2641 ttggcgcacc gtgtggccgc catcgatgaa ggacacgacc tggccctgcg ggtcgacggt
     2701 aataccccat ggggcattca ggagcgccac gccggacgct ccgtccgtgc tccccgcgac
     2761 gccggccgtg cccgcgaggg tggtgaccac gcccgcgggc gtgatgcgcc ggatcgtgtc
     2821 gttgccggtg tcggcgacgt acaggttgcc cgccgcgtcg atcgccaggc cgcgcggctg
     2881 gttgaagctc gccgcggagc ccgtgccgtc cgccgagccg caggcatacg tgccggcgaa
     2941 cgtggtcacc gtgccgtccg cggtcacctt gcggatcttg caattgcccg cttcggccac
     3001 ataggcgtcg ccgttggcgg ccacggccgt cccgctgccg aagaagccgt agaacatggc
     3061 cgtgccgccc gcgccgtcgg actgccctcc ggagtcgttg cgtccggcga tggtggtcac
     3121 gcgtccgctg ggatcgatcc gcttgaaccg ctggttgtcg aggacgaaca ggttgccctg
     3181 cgcatcgaaa cccggcatgc ccgggccttg cagccacgcg cccgagcccg tcccgtcgag
     3241 gtcgccgatc ccacccagcg cgcccgcgag caggtcgagc cggcctgcgc cggcggcgcc
     3301 cacgtgcagg gtcgccgcct ggctgaggaa cgcgcggcca ttgctcgtga cctgcagtcg
     3361 cagcagttcg ccgtcgcttg cggcagcgac gccggacagc gtcagcacct gcaggttggc
     3421 gccggggatg gcattccact gcccgccgcg atcggtgctc gattgccact ggtaggccgg
     3481 catcgcgccg ccacccgagg tccacgacga gaagcggacg tcgccgccag ccgccaccgt
     3541 ggcgtcctcc ggctgggcca cgaacaccgg gtcgctcgga ttcgtcgtga ccgacagggt
     3601 cgcgacattg ctcgtgtagg tgtggccgaa cgccgagacg accgcgcgca cctgcaggcc
     3661 gctgtcagtg agcgcgaagg cgtgcagggt cagcatctgc gaggtggcgc cggcgatgtc
     3721 ggtccacgtc tgcccgtccc ggctttcctg ccattgctgg gagatgtggg ccaggtcgaa
     3781 cgtggcgccg ccggtgaacg agacgtcatc gccgtcgaag gcggccctgg acatcggctg
     3841 caaggccatc accgggtaga cgctcagggg cgccgggaca ctcgtgacct ggccgcccgg
     3901 gcccgacacg atcacccggt attgaaggcc attggccgac aggggctcga ccacgttcag
     3961 cgtcgccgtg ttgccgccgg acacgtccgt ccaggtcgcg ccgtccgggc tctgctgcca
     4021 ctcgtagccg gtggcgttct gggcggccac cgtgaaggtc gcggtgccct cctgcaggac
     4081 ggccgcgtag aacgactgcg acgtgatcag gggcgccgcc gacggcggtg gcggcggggg
//
LOCUS       DATCRO010000883         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_482729, whole genome shotgun sequence.
ACCESSION   DATCRO010000883 DATCRO010000000
VERSION     DATCRO010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482729"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIN75_24030"
     CDS             <1..390
                     /locus_tag="VIN75_24030"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEY8977303.1"
                     /translation="VYECAAPAEAPAAWAWQFKGPEATLTDAKGAVVGRHFGGPSWAS
                     GDGATIVGKVAASAPSPERGSIPWLLLTIKSHEGAGLLAATSSVQRLDTEGGVAPASA
                     CGAANAHQVERVGYTATYVFWRAKPAA"
     gene            complement(395..817)
                     /locus_tag="VIN75_24035"
     CDS             complement(395..817)
                     /locus_tag="VIN75_24035"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8977304.1"
                     /translation="MSYHRYQGSCHCQAVKYEASIDLADGSNRCNCSLCSKARAWFTF
                     VPGERFRITQGEDALSHYRWTPASKTEPFLDYAFCSRCGIRLYGTGDAPQMGGRFYAV
                     NVPTLEGVDREALVAAPLRFVDGAHDRFDRAPEDTRLM"
     gene            905..1327
                     /locus_tag="VIN75_24040"
     CDS             905..1327
                     /locus_tag="VIN75_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8977305.1"
                     /translation="MRQDTRSTCPISTGLEIVGDRWTLLVIRDMMFAGKRHFREFLQS
                     EEGVSSNVLADRLAMLVERGLVTRADDPAHAQKAIYSLTDLGISLLPVIASLSRWTQV
                     HFPETRRPEARVFLDGDEAAIAALQQALRREHLKGGGR"
     gene            1395..1961
                     /locus_tag="VIN75_24045"
     CDS             1395..1961
                     /locus_tag="VIN75_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY8977306.1"
                     /translation="MTSHRWLLSPSLTGAALLAAAAVPASVFAATYATADDVAHRAFK
                     TATAFGEVLVTLAPADAAALAAPGGAPHGPLRTIEARQGDAVLGRVVVDSVIGKFEQI
                     DYAVALDASGKVVSVEILTYREGHGGEVRMPSWRNQFVGKTAADPIHVGSDIANISGA
                     TLSCTHVTDGVHRIVAWAAKHPIAGKAA"
     gene            1984..>2650
                     /locus_tag="VIN75_24050"
     CDS             1984..>2650
                     /locus_tag="VIN75_24050"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8977307.1"
                     /translation="MRQRARPLLGTLVEVRAQGASATAFDVAADAAFDAVAQVHALMS
                     FHESGSDLRRIARARAGERLVVHPHTAAVLRSAQRWARASGGAFDAGCAPRAVEQGWL
                     PAPADGTPPGALPFEDALELEGHAVRVHAPVWLDFGGIAKGYAVDLAVAKLRRAGVRT
                     GAVNAGGDLRVFGTLEETVHVRSPFDAADLWPVAALKDSACATSASGAVARRGVDTPD
                     GPAP"
BASE COUNT          352 a          960 c          971 g          367 t
ORIGIN      
        1 gtctacgaat gcgcggcgcc ggccgaggcg cccgccgcct gggcctggca gttcaagggt
       61 cccgaggcga cgctgaccga cgccaagggc gcggtcgtcg gccgccattt cggcgggccg
      121 tcgtgggcgt ccggcgacgg cgccaccatc gtcggcaagg tcgcggccag cgccccgtcg
      181 cccgagcgcg gctcgatccc gtggctgctg ctcacgatca agtcgcacga gggggccggc
      241 ctgctggcgg ccacgtccag cgtccagcgc ctggacaccg agggcggcgt cgcgcccgcc
      301 agcgcgtgcg gggcggcgaa cgcgcaccag gtcgagcgcg tcggctacac ggccacgtac
      361 gtcttctggc gcgcgaagcc ggcggcctga cgcctcacat cagccgcgtg tcttcgggcg
      421 cgcggtcgaa acggtcgtgc gcgccatcga cgaagcgcag cggcgccgcg accagggcct
      481 cgcggtcgac cccttccagc gtgggcacgt tgaccgcgta gaagcgcccg cccatctggg
      541 gcgcatcgcc ggtgccgtac aggcggatgc cgcagcgcga gcagaacgcg tagtcgagga
      601 agggctccgt cttgctggcg ggcgtccagc ggtagtgcga cagcgcgtcc tcgccctgcg
      661 tgatgcggaa gcgttcgccc ggcacgaacg tgaaccacgc ccgggccttg ctgcacagcg
      721 agcagttgca gcggttgctg ccgtcggcca ggtcgatcga ggcctcgtac ttcacggcct
      781 ggcagtggca gctgccctgg tagcggtggt agctcatggt cggtctcctt gactggtttc
      841 gttttgagag cagtcatcgt acgcagaccg atcttcaaat gcaagcggtt ttagaatgcg
      901 gcccatgcgc caggacaccc gctccacctg cccgatcagc acgggcctcg agatcgtcgg
      961 tgaccgctgg accttgctgg tgatccgcga catgatgttc gcgggcaagc gccacttccg
     1021 cgagttcctg cagtccgagg aaggcgtgtc gagcaacgtg ctggccgacc ggctggcgat
     1081 gctcgtcgaa cgcggcctgg tcacgcgcgc cgacgacccc gcccacgcgc agaaggcgat
     1141 ctacagcctc accgacctgg gcatctcgct gctgccggtg atcgcctcgt tgagccgctg
     1201 gacgcaggtg cacttccccg agacgcgccg tcccgaggcc cgcgtgttcc tcgatggcga
     1261 cgaggcggcg atcgcggcgc tgcagcaggc gttgcggcgc gagcacctga agggcggcgg
     1321 tcgctgacag ccgggttgcc ggtagatgag aacggtccgc actatcattc gagtccgcgc
     1381 atcttccgat ccccatgacc tcgcaccgct ggctcctgtc tccctcgctc accggcgccg
     1441 ccctgctcgc tgcggccgcc gtgccggcca gcgtgttcgc ggccacctac gccaccgccg
     1501 acgacgtcgc gcaccgcgcg ttcaagacgg cgacggcgtt cggcgaggtg ctggtcacgc
     1561 tggcgcccgc cgatgccgcc gcgctggccg cgcccggcgg tgcgccgcac gggccgctgc
     1621 gcacgatcga ggcgcgccag ggcgacgcgg tgctggggcg cgtggtcgtg gactcggtca
     1681 tcggcaagtt cgagcagatc gactacgcgg tggcgctgga cgcgtcgggc aaggtggtct
     1741 cggtcgagat cctcacgtac cgggagggcc atggcggcga ggtgcgcatg ccgtcgtggc
     1801 gcaaccagtt cgtcggcaag acggcggccg atccgatcca cgtgggctcg gacatcgcca
     1861 acatcagcgg cgccacgctc agctgcacgc acgtgaccga cggcgtgcac cgcatcgtcg
     1921 cgtgggcggc gaagcacccg atcgcgggca aggccgcgtg acgatgaagg cggccgcgac
     1981 gtgatgcgcc aacgggcgcg cccgctgctg ggcacgctgg tggaggtgcg cgcccagggc
     2041 gccagcgcca ccgcgttcga cgtcgccgcc gacgccgcgt tcgacgcggt cgcgcaggtg
     2101 cacgcgctca tgagcttcca cgaatcgggc agcgacctgc gccgcatcgc gcgcgcccgc
     2161 gccggcgagc gcctcgtcgt ccatccgcat accgccgccg tgctgcgcag tgcgcaacgc
     2221 tgggcgcgcg cgagcggcgg cgcgttcgac gccggctgcg cgccgcgcgc ggtcgaacag
     2281 ggctggctgc cggcccccgc ggacggcaca ccgcccgggg cgctgccgtt cgaggacgcg
     2341 ctggagctcg agggccatgc cgtgcgcgtg cacgcgccgg tctggctgga cttcggcggc
     2401 atcgccaagg gctacgcggt cgatctcgcc gtggccaagc tgcggcgcgc cggcgtgcgc
     2461 accggggccg tcaacgcggg cggcgacctg cgggtgttcg gcacgctgga agaaacggtg
     2521 cacgtgcgct cgccgttcga cgccgccgac ctgtggccgg tggccgcgct caaggactcg
     2581 gcctgcgcga cgagcgccag cggagccgtc gcgcgccgcg gcgtcgacac ccccgacggc
     2641 cccgcgccgc
//
LOCUS       DATCRO010000884         5125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_639703, whole genome shotgun sequence.
ACCESSION   DATCRO010000884 DATCRO010000000
VERSION     DATCRO010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639703"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VIN75_24055"
     CDS             complement(<1..735)
                     /locus_tag="VIN75_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8977308.1"
                     /translation="MTNEREATTPVNRPQASVWRLAGRQVWRDLVAGELKLLLWAVVL
                     AVGALTAVGFFADRMRAGLVRDAAQLLGGDAVVLGDKPLAPAFAQEALRRGFVVGQAA
                     TFPSMARAGAAQGGAAKLAAVKAVDAAYPLRGSIDVRFGEAAPVRSLHQAPAAGTAWI
                     DPALANALSLKVGDSLELGDTTLRIAALIAIEPDRGAGFSALAPRVLRRDVGTPKAAS
                     LGVLAGAFLGFAALMVIAARDVKLGLI"
     gene            888..1751
                     /locus_tag="VIN75_24060"
     CDS             888..1751
                     /locus_tag="VIN75_24060"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8977309.1"
                     /translation="MDTRYAPVFLSHGSPMTALESGPAGAFWRDLGRAIDARAAGGGA
                     APAAIVALSAHTLARGAVTLAAARHEAVHDFGGFPRALYELRYDVAGVPCLAPHVEQL
                     LQWDGIEVEHADAGGLDHGIWVPLRDIRPRADVPVLPVAFPPDWTPARLFALGAALRP
                     LVDEGVWIVGTGSITHNLRLGLPRAEPVPEIPASAAFRAWFAERSAARDWASLLNYRA
                     LAPGAATMHPTDEHLLPWFIAAGAGGEADAPIRLHASVAGGHLGMDAYAFGPDARALA
                     DDVAAAAAATA"
     gene            1806..2426
                     /locus_tag="VIN75_24065"
     CDS             1806..2426
                     /locus_tag="VIN75_24065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977310.1"
                     /translation="MPGIAPLARYEISTARLRLVAGDPGIAPAVHQFHVRNRQHFARW
                     DPPTGDAFYTLEAQAARVASGITAFNNDTAYRWWLVDATRCGGARLPSSDVEVIGSLN
                     FSQVSRGAFHSAMLGYALDEAHVGQGLMAEAIAAGLEEMFSPRVNLHRVQANYRPENL
                     RSARVLERAGFHKEGLAPQYLYIDGAWRDHIMTSIRNPAFVAPDGW"
     gene            complement(2642..4171)
                     /locus_tag="VIN75_24070"
     CDS             complement(2642..4171)
                     /locus_tag="VIN75_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977311.1"
                     /translation="MIRLSIKTRLLLLVGSLLFLFVAAAGFGVLRMKAGNESLGSLYN
                     ERVVSTERLKHVSDGYNEIIDIAHKVGAAQMTAAVGAAQVKAAQAKAADNWKAYLATA
                     IDDDERPLVGQATPLMAKADAVTAELLAALAKDDVDGVFKLASNGLYPSVDPLSDVID
                     KLATLQLRGAANEYAAAQKLYGVVLWTTLAIGAAVLVVAVLAGVGLVRSITSGIGVAV
                     KVAQTVAAGDLGSVIVIDRRDEIGTMLGALKAMNESLVAIVGDVRSASESIATGSSQI
                     AHGNSDLSQRTEEQASNLEQTAASMEQLTATVKHNADTARRASELAEGASMVAAQGGE
                     VVGQVVSTMDDITDSSRKITDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA
                     SEVRSLAQRSATAARQIKVLIGESVGKVENGTRLVADAGRTMGDIVEQVRRVSAMINE
                     ISDASDEQSRGIGQIGDAVAQLDQVTQQNAALVEESAAAAESLRVQADTLSRTVATFR
                     L"
     gene            complement(4291..>5125)
                     /locus_tag="VIN75_24075"
     CDS             complement(4291..>5125)
                     /locus_tag="VIN75_24075"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8977312.1"
                     /translation="YYAGASVLLHEADFADMAWAYFERAAADNVVHCEVFFDPQTHTA
                     RGVDMGVVIRGLDRAARRAEAELGLSVRLILCFLRHLSEDDALATLEAALPYRDKFIG
                     VGLDSSEMGHPPEKFARVFARAREAGLRLVAHAGEEGPPAYIRSALDVLHVERIDHGV
                     RCLEDPDLVARLARERVPLTVCPLSNVKLCVFDQLGRHNIPALLAAGLCVTVNSDDPA
                     YFGGYVNDNFVQLFESLPQLTAFDAYTLLRNSFDAAFVDDEDKALWTEELDSVFAANE
                     P"
BASE COUNT          687 a         1941 c         1778 g          719 t
ORIGIN      
        1 gatgagcccc agcttgacgt cgcgcgccgc gatcaccatc agcgccgcga agcccaggaa
       61 cgcgcccgcg aggacgccga ggctggccgc cttgggcgtt cccacgtcgc gccgcagcac
      121 gcgcggcgcc agcgccgaga agcccgcgcc gcgatcgggc tcgatcgcga tcagcgcggc
      181 gatgcgcagc gtggtgtcgc ccagctcgag cgagtcgccc accttgagcg acagcgcatt
      241 ggccagcgcc ggatcgatcc aggcggtgcc cgcggccggc gcctgatgca gcgagcgcac
      301 cggcgcggcc tcgccgaagc ggacatcgat gctgccgcgc agcggatagg ccgcatcgac
      361 cgccttgacc gccgccagct tggccgctcc gccctgcgcc gcgccggcgc gcgccatgct
      421 ggggaacgtg gcggcctgcc cgacgacgaa gccgcgccgc agcgcctcct gcgcaaacgc
      481 cggcgccagc ggcttgtcgc ccagcacgac ggcgtcgccg cccagcagct gcgcggcgtc
      541 gcgcacgagg ccggcgcgca tgcggtcggc gaagaagccc accgccgtca gcgcgcccac
      601 cgccagcacc accgcccaca gcagcagctt gagctcgccg gcaaccagat cgcgccagac
      661 ctggcggccg gccagccgcc agacggaggc ctgcggccgg ttcacggggg tcgtcgcttc
      721 acgctcgttc gtcatggccg caacggtaca cccaagccgt gaaggccgcc gcgcgcggaa
      781 cggccgattc gggccgtgca atgccgccgg cgacccgtca acgcggttga acgccatcgc
      841 caatcgcttc gaacggcgcc gcgcgggccc ggtgcttcaa tgaacgcatg gacacccgct
      901 acgcccccgt cttcctctcg cacggctcgc cgatgacggc gctggaatcc ggccccgccg
      961 gcgcgttctg gcgcgacctg gggcgcgcga tcgacgcccg ggccgccggc ggcggcgcgg
     1021 cgcccgcggc catcgtcgcg ctgtccgcgc acacgctcgc gcgcggcgcc gtcacgctcg
     1081 ccgccgcgcg ccacgaggcc gtgcacgact tcggcggctt cccgcgcgcc ctctacgagc
     1141 tgcgctacga cgtcgccggc gtgccctgcc tcgcgccgca cgtcgagcag ttgctgcaat
     1201 gggacggcat cgaggtcgag cacgccgacg ccggcggcct cgaccatggc atctgggtac
     1261 cgctgcgcga catccgcccg cgcgccgacg tccccgtgct gcccgtcgcc ttcccgccgg
     1321 actggacgcc ggcgcgcctg ttcgcgctcg gcgccgcgct gcggccgctc gtcgacgagg
     1381 gcgtgtggat cgtgggcacc ggatcgatca cccacaacct gcgcctgggc ctgccgcgtg
     1441 cggagccggt gcccgagatc cccgccagcg ccgccttccg cgcctggttc gccgagcgct
     1501 cggcggcgcg cgactgggcg tcgctgctga actaccgcgc gctcgccccc ggcgccgcca
     1561 ccatgcaccc gaccgacgag cacctgctgc cctggttcat cgccgcgggc gccggcggcg
     1621 aggcggacgc gcccatccgg ctgcacgcga gcgtggccgg cggccacctg ggcatggacg
     1681 cgtacgcgtt cggacccgac gcccgcgcgc tggccgacga tgtcgccgcc gccgcggccg
     1741 ccacggcctg aggccgcgtc gccgggtacg atgcgggtcc gttccgcatc gtccccgctt
     1801 cgcccatgcc gggtatcgct cccctcgccc gctacgagat ctccaccgcg cgcctgcgcc
     1861 tcgtcgccgg cgaccccggc atcgcgccgg ccgtccacca gttccacgtc cgcaaccgcc
     1921 agcatttcgc gcgctgggat ccgcccacgg gcgacgcctt ctacacgctc gaggcccagg
     1981 ccgcgcgcgt ggcgagcggc atcaccgcgt tcaacaacga caccgcctac cgctggtggc
     2041 tggtggacgc cacgcgctgc ggcggcgcgc gcctgccctc gtcggacgtc gaggtgatcg
     2101 gcagcctgaa cttcagccag gtctcgcgcg gcgcgttcca cagcgcgatg ctgggctacg
     2161 cgctcgacga ggcgcacgtc ggccagggcc tgatggccga ggcgatcgcc gccggcctcg
     2221 aggagatgtt ctcgccgcgc gtgaacctgc accgcgtgca ggccaactac cggcccgaga
     2281 acctgcgcag cgcgcgcgtg ctcgaacgtg ccggcttcca caaggaaggc ctggcgccgc
     2341 agtacctgta catcgacggc gcctggcgcg accacatcat gacgtcgatc cgcaacccgg
     2401 cgttcgtggc gccggacggt tggtaggaag acggcgggcc ggctccgtcc gtcccgacca
     2461 agcgccccgg cgtgcgagga tcccgcgact gcgctcagga tgacggcccg gcgggagact
     2521 gcttcgagcc gcctgccgag gcggccgcaa cccgcgaggc gccgtcatcc tgcgcgcggg
     2581 aagcgcagcc gcaggatcca cgcgtgccca agcgccctgg ctcgcatggc cgcgcggcgc
     2641 cttacagcct gaacgtcgcc accgtccgcg acagcgtgtc ggcctgcacg cgcaggctct
     2701 cggccgcggc ggccgattcc tcgacgagcg cggcgttctg ctgcgtgacc tggtcgagct
     2761 gcgcgacggc gtcgccgatc tggccgatgc cgcgcgactg ctcgtcgctc gcgtcgctga
     2821 tctcgttgat catcgcgctc acgcgcctga cctgctcgac gatgtcgccc atcgtgcggc
     2881 cggcgtcggc caccaggcgg gtgccgttct ccaccttgcc cacgctctcg ccgatcagca
     2941 ccttgatctg cctggcggcc gtcgccgagc gctgggccag cgaacgtacc tcgctggcga
     3001 cgaccgcgaa gccgcggccc tgctcgccgg cgcgcgccgc ttccaccgcg gcgttcagcg
     3061 cgaggatgtt ggtctggaac gcgatgccgt cgatcacgcc gatgatgtcg gtgatcttgc
     3121 gcgaactgtc ggtgatgtcg tccatcgtcg agacgacctg tcccaccacc tcgccgccct
     3181 gggccgccac catcgacgcg ccctcggcca gctcgctggc gcggcgcgcg gtgtcggcgt
     3241 tgtgcttgac ggtggcggtc agctgctcca tcgaggccgc ggtctgctcc aggttgctcg
     3301 cctgttcttc cgtgcgctgg ctgaggtcgc tgttgccgtg cgcgatctgc gacgacccgg
     3361 tcgcgatcga ttccgacgcg ctgcgcacgt cgccgacgat ggccaccagg ctctcgttca
     3421 tcgccttgag cgcgcccagc atcgtgccga tctcgtcgcg gcggtcgatc acgatgaccg
     3481 agccgaggtc gcccgcggcc accgtctgcg ccaccttcac ggccacgccg atgccgctgg
     3541 tgatcgagcg gaccaggccc acgcccgcga ggacggccac gacgagcacg gccgcgccga
     3601 tggccagcgt cgtccacagc accacgccgt agagcttctg ggcagcggcg tactcgttcg
     3661 cggcgcccct caactgcagg gtggcgagct tgtcgatgac gtccgagagc gggtcgacgg
     3721 acgggtacag gccgttggat gcgagcttga agacgccgtc gacgtcgtcc ttggccagtg
     3781 cggccagcaa ttccgccgtc accgcgtcgg ccttggccat cagcggggtg gcctgcccga
     3841 ccagcggacg ctcgtcgtcg tcgatcgcgg tggcgaggta cgccttccag ttgtcggccg
     3901 ccttggcctg cgccgccttg acctgggcgg cgccgaccgc cgccgtcatc tgtgccgcgc
     3961 ccaccttgtg cgcgatgtcg atgatctcgt tgtagccgtc gctcacgtgc ttgaggcgct
     4021 ccgtcgacac cacgcgctcg ttgtagagcg agcccagcga ctcgttgccg gccttcatcc
     4081 gcagcacgcc gaagccggcg gcggcgacga acaggaacag caggctgccg acgaggagca
     4141 ggaggcgggt cttgatgctc aggcggatca tggcgcggtc tcgaagtgcg cgccggggtg
     4201 aggcgcccct gtcacttcgg ccgttgcggc caaaccttaa cgggacctgt gtcgtggctg
     4261 aaccggaccg agatctccct acacgtccga tcagggttcg ttggccgcga acaccgaatc
     4321 gagctcctcc gtccagagcg ctttgtcctc gtcgtcgacg aaggccgcgt cgaagctgtt
     4381 gcgcaacagc gtataggcgt caaaggcggt cagttgcggc aacgattcga acagttggac
     4441 gaagttgtcg ttgacatagc caccgaagta ggcgggatcg tcggagttga cggtgacgca
     4501 cagcccggca gcgagcagcg ccgggatgtt gtgccggccg agctggtcga acacgcacag
     4561 cttgacgttg gacagcgggc agaccgtgag cggcacgcgc tcgcgcgcca ggcgcgccac
     4621 caggtcgggg tcctcgaggc agcgcacgcc atggtcgatg cgctcgacgt gcagcacgtc
     4681 cagcgccgag cggatgtagg ccggcggccc ctcctcgccc gcgtgcgcga ccaggcgcag
     4741 gccggcctcg cgggcacggg cgaagacccg cgcgaacttc tcgggcggat ggcccatctc
     4801 gctggaatcg aggccgacgc cgatgaactt gtcgcggtac ggcagcgccg cctccagcgt
     4861 cgcgagcgcg tcgtcctcgc tgaggtggcg caggaagcac aggatcaggc gcacgctcag
     4921 gcccagctcg gcctccgcgc gccgggccgc gcggtccagg ccgcggatga cgacgcccat
     4981 gtcgacgccg cgcgccgtgt gggtctgcgg gtcgaagaag acctcgcagt ggacgacgtt
     5041 gtcggccgcg gcgcgctcga agtaggccca ggccatgtcg gcgaagtcgg cctcgtgcag
     5101 cagcacgctg gcgccggcgt agtag
//
LOCUS       DATCRO010000885         8441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_336651, whole genome shotgun sequence.
ACCESSION   DATCRO010000885 DATCRO010000000
VERSION     DATCRO010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336651"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1189
                     /gene="tehA"
                     /locus_tag="VIN75_24080"
     CDS             206..1189
                     /gene="tehA"
                     /locus_tag="VIN75_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEY8977313.1"
                     /translation="MTTQRVPVPVSFFSIPVGLLAFANTWHVAARLWHLSGDVAQAFT
                     FTGLALWVGLLGLYARKWFTHRAEAVAELQHPLHSAFAALVPVSSMLAAVALIPLWRD
                     LATGLFVVALAAQLALGLALNGRLWMGGRPQELVTPAMYLPSVAQSFVAATASAAFGW
                     HQLGMMFFGVGLLAWLALESLIVQRAAVGTPLPEALRPLLGIQLAPAVVGGGSWLALT
                     TGTPDMFAWLLLGYGLYQALLLLRLLPWIRQQAFAPAYWAFSFGTAALPALAMRMVER
                     GASGLVADLAPVLFAAANMVFVLLIAGTLRLLARGTLLPLITPPPAAVATP"
     gene            complement(1215..3410)
                     /locus_tag="VIN75_24085"
     CDS             complement(1215..3410)
                     /locus_tag="VIN75_24085"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8977314.1"
                     /translation="MRTLASLRALLGGLALAAGLVAPAFAAAPGQPVAPVHPVTDTYF
                     GTPVVDDYRYMEDLDNPEVKAWMRGQADYTRGQLDALPGRGPLLDRVHALLNADLQRG
                     AFIQRGQRLFYKAIQPGAPLPRLYWRDGAHGEEHLLVDPGALGKGTATHYALDFFMPS
                     WDGKRIAYGLSAGGSEKSVLHVMDVQTGAILSEAIDRTSNSRVSWRPDNQSFYYLRYP
                     KPTPDLPPDRTMFNGRTYLHVLGAHADGEGDAAVFGRGVSPGLDVPEGQGTWLVTSPG
                     SKYALAIANHNMDDNPSTVYVAPLAQVKGAGTPWRKIADANDGVTAFHLRGDTLYLLA
                     QKDAPRYRLLSLPLARPVLSRATVVLPEGDGVLTDVEIARDGLYARVRDGAVSHVHRI
                     SFDGRLSQPVPLPFEGDVSALVTDEREPGALISLRGWLQSTRTLAYDPARGRADDTGL
                     NPPSSIDTSAYAADEVFAIGDDGTRIPLSILHRKDMVADGSHPTILSGYGAYGISQEP
                     SFVPTNLAWLERGGVLAYAHIRGGGEYGEGWHQAGYKQTKLNTVSDFAACGQYLVDHR
                     VTTSARLAGLGGSAGGITVGGALTRHTALFGVILDLVGMSDTLRAENSPNGPPNIVEF
                     GTVKDEAGFHGLYAMSAYAHVRDGTPYPAVLFSTGANDPRVSPWQMGKMAARVQGATS
                     SGKPALLRIDYDAGHGLGSSSAQRENQIADLWSFALWQMGDPAFQPAAQ"
     gene            3594..5048
                     /gene="sbcB"
                     /locus_tag="VIN75_24090"
     CDS             3594..5048
                     /gene="sbcB"
                     /locus_tag="VIN75_24090"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEY8977315.1"
                     /translation="MTDFSFFWHDYETFGRVPRRDRPAQFAGIRTDADLNEVGQSVMH
                     YCRPAPDSLPDPESCLLTGIVPQTCLELGLPEHAFAAQVEAQLGQPGTIGVGYNTIRF
                     DDEVTRHLFWRNLIDPYAREWQNECGRWDLLDVVRATWALRPEGIEWPKHEDGRPSFK
                     LEHLTQANGIAHEAAHDALSDVRATIALARLIKERQPRLWEFCLKLRRKEAVWTEIGA
                     HRPFLHISGMFGVEQGCMAIVYPLAQHPTNKNELLVWDLAHDPRELAGLDSETLRLRM
                     FTRTADLPEGVARLPIKSIHANKSPFVVGNLKTLTPATAQRWSIDVERALAHAQAATD
                     VAALMQGAWREVYARPAPSSPVDVDEDLYGGFVGNDDRRKLERLRRLTGEQLAGQRVA
                     FDDPRLEELLFRYRARNFGDTLSAQENERWLDHCSARLHEGAGGSMTLSAFFERIDQL
                     NEHAEETGDERAAGILGALYDYAEQIAPPLREGA"
     gene            5144..6124
                     /locus_tag="VIN75_24095"
     CDS             5144..6124
                     /locus_tag="VIN75_24095"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8977316.1"
                     /translation="MRVAIAGAGAVGCYYGGMLARAGVPVTLIGRPRHVDAMRRDGLR
                     LTTAAFDEHFPVEASTEPAAVAGAALVLVCVKSPDTESTGRELAPHLAPDACVLSLQN
                     GVDNAARLQAVLGRPVAPAVVYVASEMAGPGHVRHMGRGELVIPSDIGAATSTEAADA
                     VAALGASRAPVPLAESGLASPDIVALFARAGVPVELSDNVLGALWGKLLLNCGYNAIS
                     AITDIPYGRMVALPGVARTVHDAVAECLAVAAAMDVSIPGDVWAAVERIATSMPGQVS
                     STCQDLRRGKPTEIDHLNGYVVRQGDRLGIPVPLNRALHALVKALEAKAR"
     gene            complement(6289..>8441)
                     /locus_tag="VIN75_24100"
     CDS             complement(6289..>8441)
                     /locus_tag="VIN75_24100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY8977317.1"
                     /translation="NLFHHHRQGASTITQQVARNFFLSRKQTWSRKFAEALLALKIER
                     HLSKDQILELYMNQIYLGQHAYGFEAASLTYFGKPSRQLTPAEAAMLAGLPKNPGGAD
                     PTDHFEKAKARQLVVLMRMHETGVLDDAQYETAKKQPLKITGHWAPPVSAEYLAEMVR
                     QQVVQRFGEAAYSQGLKVTTSLVSADQAAAQAAVRKGVIDFDRRQAYRGPEDNEDLPD
                     SDADIDTAAAAALHDHPDDDDLRVAVVTHASPKEVVAILASGETLHLTGDALRGVGVL
                     AALRPKAAEDVAITRGAIIRVMRTTGDSSYKSEWRLTQWPDVEGALVSLDSATGQVRA
                     LVGGFDFSHSQFNHVTSAWRQPGSSFKPFIYSAALEHGIQPATQIMDAPINIDDSLTG
                     GKHWDPKDDDEPLMGPISLRAALAKSKNLVSIRVVQNLGTSVVKNWAAQFGLSPEKQP
                     DNLTLALGAGSVTPMQQARAYAAFANGGFGVTPRFIEKITDAQGKVLFEAPKLPDTPS
                     EDARIIPARNAFVMASLLNEVARTGTAAKAQSTLKRPDLYGKTGTTNDAVDTWFAGFQ
                     PRITTVVWMGRDDNTSLGGREFGATLALPIWIDFMRSALKDVPVTTMQQPAGVQDVQG
                     DWLYDEFAGGGYIAYLGMEPGNPPSVMVAGAPRDAASQAAIDATPMQPTSPGATILQP
                     APPPPPAPAASRPKPAATISPSDDPFSRLAPALR"
BASE COUNT         1099 a         2940 c         3129 g         1273 t
ORIGIN      
        1 ccaccggatc gtgcggcgcc gcgaatgccg cgccgaacag gcagcgcagc tccatgcggc
       61 cgcgcgggtc gagtagggtg acgaggtcgc tcagcggatc catgggagac gttcgagcaa
      121 ttgatatgga ttaaccggca ttcaagatct tgattgtggc gaacagaatc gattccgtcg
      181 atcacctcct ctccggagtc ctgccatgac aacccaacgc gtccccgtcc cggtctcgtt
      241 cttcagcatc cccgtggggc tgctcgcgtt cgccaacacc tggcacgtgg cggcgcgcct
      301 gtggcacctg tccggcgacg tcgcccaggc gttcacgttc acgggcctgg ccctgtgggt
      361 gggcctgctg gggctgtacg cccgcaagtg gttcacgcat cgcgcggagg cggtcgccga
      421 gctgcagcac ccgctccact cggcgttcgc ggcgctggtg cccgtctcga gcatgctggc
      481 ggcggtggcc ctgatcccgc tctggcgcga cctggcaacc ggactgttcg tggtggcgct
      541 cgcggcccag ctggcattgg gcctcgcgct gaacggacgg ctgtggatgg gcggtcgccc
      601 gcaggaactc gtgacgccgg cgatgtacct gcccagcgtc gcgcagagct tcgtcgccgc
      661 gaccgcgtcg gccgcgttcg ggtggcacca gctcgggatg atgttcttcg gcgtcgggct
      721 gctggcgtgg ctcgcgctcg aatcgctgat cgtgcagcgg gcggcggtcg gcacgccgtt
      781 gcccgaggcg ttgcgcccgc tgctgggcat ccagctcgcg ccggcggtgg tcggcggcgg
      841 cagctggctg gccctgacga caggcacgcc agacatgttc gcgtggctgc tgctgggcta
      901 cggcctgtac caggcgctcc tgctgctgcg cctgctgccc tggatccgcc aacaggcgtt
      961 cgcgccggcg tactgggcct tcagcttcgg caccgcggcg ctgccggcgc tggcgatgcg
     1021 gatggtcgaa cgcggggcgt ccggcctcgt cgccgacctg gcccccgtgc tgttcgcggc
     1081 ggccaacatg gtcttcgtgc tgctgatcgc gggcacgctg cggctgctgg cccgggggac
     1141 gcttcttccc ttgatcacgc ccccgccggc ggcggtcgcg accccctgat ggctgcgcgt
     1201 caccggatgg ggcgtcactg cgccgccggc tggaacgccg ggtcgcccat ctgccatagc
     1261 gcgaacgacc acaggtcggc gatctggttc tcgcgctggg cgctgctcga gcccaggcca
     1321 tggcctgcgt cgtagtcgat gcgcagcagc gccggcttgc cgctggaggt ggcgccctgg
     1381 acgcgcgcgg ccatcttgcc catctgccac ggcgacacgc gcgggtcgtt ggcgccggtg
     1441 ctgaacagga cggcggggta gggcgtgccg tcgcgcacgt gggcatatgc gctcatcgcg
     1501 tacaggccgt ggaagccggc ttcgtccttg accgtgccga actcgacgat gttgggcggc
     1561 ccgttgggcg agttctcggc gcgcagcgtg tcggacatgc ccacgagatc gaggatgacg
     1621 ccgaacagcg cggtgtgccg cgtcagtgcg ccgccgacgg tgatgccgcc ggcgctgccg
     1681 cccagcccgg ccaggcgcgc ggaggtcgtg acgcgatggt cgaccaggta ctggccgcag
     1741 gcggcgaaat cgctgaccgt gttgagcttg gtctgcttgt agcccgcctg gtgccagccc
     1801 tcgccgtact cgccgccccc gcggatgtgg gcgtaggcca gcacgccgcc gcgctcgagc
     1861 caggcgaggt tggtcggcac gaagctgggc tcctgcgaga tgccgtaggc gccgtagccg
     1921 ctgaggatcg tcgggtggct gccgtcggcc accatgtcct tgcggtgcag gatggacagc
     1981 gggatgcgcg tgccgtcgtc gccgatggcg aacacctcgt cggccgcgta ggccgaggtg
     2041 tcgatggacg acggcgggtt caggccggtg tcgtcggcgc ggccgcgcgc cgggtcgtag
     2101 gccagcgtgc gcgtggactg cagccagccg cgcaggctga tcagcgcgcc cggctcgcgc
     2161 tcgtcggtca ccagcgcgct gacgtcgccc tcgaagggta gcggcacggg ctggctcagg
     2221 cggccgtcga acgagatgcg atgcacgtgc gacaccgcgc cgtcgcggac gcgcgcgtac
     2281 agcccgtcgc gcgcgatctc gacgtcggtc agcacgccgt cgccctcggg cagcaccacg
     2341 gtcgcgcgcg agagcaccgg cctcgccagc ggcagcgaca gcaggcggta gcgtggcgcg
     2401 tccttctgcg cgagcaggta caacgtgtcg ccgcgcaggt ggaacgccgt gacgccgtcg
     2461 ttcgcgtcgg cgatcttgcg ccacggcgtg ccggcgccct tgacctgcgc gagcggcgcc
     2521 acgtagaccg tggacgggtt gtcgtccatg ttgtggttgg cgatggccag cgcgtacttc
     2581 gagcccggcg aggtcacgag ccacgtgccc tggccctcgg gcacgtccag gccgggcgac
     2641 acgccgcggc cgaacacggc ggcatcgccc tcgccgtcgg cgtgggcgcc cagcacgtgc
     2701 aggtaggtgc ggccgttgaa catggtccga tccggcggca ggtccggcgt gggcttggga
     2761 tagcgcaggt agtagaacga ctggttgtcg ggccgccacg acacgcggct gttggacgtg
     2821 cggtcgatgg cctcggacag gatcgcaccc gtctgcacgt ccatcacgtg cagcacgctc
     2881 ttctcggagc cgcccgcgga caggccgtac gcgatgcgct tgccgtccca cgacggcatg
     2941 aagaagtcga gcgcgtagtg cgtggccgtg cccttgccga gcgcgcccgg gtcgaccagc
     3001 aggtgctcct cgccgtgcgc gccatcgcgc cagtacagcc gcggcagcgg cgcgccgggc
     3061 tggatcgcct tgtagaacag gcgctggccg cgctggatga aggcgccgcg ctgcaggtcg
     3121 gcattgagca gggcgtgcac gcggtcgagc agcggcccgc ggccgggcag ggcatcgagc
     3181 tggccgcggg tgtagtcggc ctgcccgcgc atccacgcct tcacctcggg gttgtccaga
     3241 tcctccatgt agcggtagtc gtcgacgacc ggcgtgccga agtaggtgtc ggtgaccggg
     3301 tgcaccgggg cgaccggctg gccaggcgcc gcggcgaagg cgggcgcgac gaggccggcg
     3361 gcgagcgcga ggccgccgag cagggcgcga agggaggcga gggttcgcat gggatgggtg
     3421 ttcggatggc gacggcgggc gagtttacgt gcgctcccga agcgcacttc gcacgcggat
     3481 ttcgccgtgc cgccagtgtc cttcgaaccg cgccgggacg cccggcgccg gaagaccgaa
     3541 aatgcggcaa tcggcgcgcg tcgcgcccga gcactcgaag gtatcttcct cgcatgaccg
     3601 acttcagctt cttctggcac gactacgaaa ccttcggccg cgtgccccgg cgcgaccgcc
     3661 ccgcgcagtt cgccggcatc cgcaccgatg cggacctgaa cgaggtcggc cagtcggtga
     3721 tgcactattg ccgcccggcg cccgattcgt tgccggatcc cgagtcctgc ctgctcacgg
     3781 gcatcgtgcc gcagacctgc ctcgagctgg gcctgcccga gcacgcgttc gcggcgcagg
     3841 tcgaggccca gctgggccag ccgggcacca tcggcgtcgg ctacaacacc atccgcttcg
     3901 acgacgaggt cacccgccac ctgttctggc gcaacctcat cgacccgtat gcgcgcgaat
     3961 ggcagaacga gtgcggccgc tgggacctgc tggacgtcgt gcgcgccacc tgggcgctgc
     4021 ggcccgaagg catcgagtgg ccgaagcacg aggacggccg gccgtcgttc aagctggagc
     4081 acctgaccca ggccaacggc atcgcgcacg aggccgcgca cgacgcgctg tccgacgtgc
     4141 gcgccaccat cgcgctggcg cgcctgatca aggagcggca gccgcgactg tgggagttct
     4201 gcctgaagct gcgccgcaag gaggcggtgt ggacggagat cggcgcccac cggccgttcc
     4261 tgcacatctc gggcatgttc ggcgtggagc agggctgcat ggccatcgtg tatccgctgg
     4321 cgcagcatcc caccaacaag aacgagctgc tggtgtggga cctcgcgcac gacccgcgcg
     4381 agctcgccgg cctcgactcc gagacgctgc gcctgcgcat gttcacgcgc acggccgacc
     4441 tgcccgaggg cgtcgcgcgg ctgccgatca agtccatcca cgccaacaag tcgcccttcg
     4501 tcgtgggcaa cttgaagacg ctcacgcccg cgacggcgca gcgctggtcc atcgacgtcg
     4561 agcgcgcgct ggcacacgcg caggcagcaa cggatgtcgc cgcgctgatg cagggcgcct
     4621 ggcgcgaggt ctatgcgcgc cccgcgccgt cgtcgccggt ggacgtcgac gaggacctgt
     4681 acgggggctt cgtcggcaac gacgatcgcc gcaagctcga gcgcctgcgc cgcctgacgg
     4741 gcgagcagct cgccggacag cgcgtggcct tcgacgaccc gcgcctggag gaactgctgt
     4801 tccgctaccg cgcgcgcaac ttcggcgaca cgctcagcgc gcaggagaac gagcgctggc
     4861 tcgaccactg cagcgcgcgc ctgcacgaag gcgccggcgg ctcgatgacg ctgtccgcgt
     4921 tcttcgagcg catcgaccag ctcaacgagc acgccgagga gacgggcgac gagcgtgccg
     4981 ccgggatcct gggcgcgctg tacgactacg ccgagcagat cgcgccgccg ctgcgcgagg
     5041 gcgcatgacg gccgcggcgg caggggatgc ctcgcgcggc ggcgcgggcg ggcgcgctgg
     5101 cgcaagcgcg gacgcaggcg cgaacgctcg cggggccgac cgcgtgcgcg tcgcgatcgc
     5161 cggcgccggc gccgtcggct gctactacgg cggcatgctc gcgcgcgccg gcgtgcccgt
     5221 caccttgatc gggcggccgc ggcacgtcga cgccatgcgg cgcgacgggc tgcgcctgac
     5281 cacggcggcc ttcgacgaac actttcccgt cgaggccagc accgagcccg ccgcggtggc
     5341 cggcgccgcg ctggtgctgg tgtgcgtcaa gtcgcccgac accgagtcca ccggacgcga
     5401 gctcgccccg cacctggcgc ccgacgcctg cgtgctgagc ctgcagaacg gcgtcgacaa
     5461 cgcggcgcgc ctgcaggcgg tgctcggccg gccggtcgcg ccggcggtcg tctatgtcgc
     5521 cagcgagatg gccggccccg gccatgtgcg ccacatgggc cgcggcgaac tggtcattcc
     5581 gtcggacatc ggcgccgcga cgtccaccga ggccgccgac gccgtggcgg ccctcggcgc
     5641 gagccgggcc cccgtcccgc tggcagagtc cggcctggcg tcgcccgaca tcgtcgcgct
     5701 gttcgcccgt gccggagtgc cggtcgaact gtcggacaac gtgctcggcg cgctgtgggg
     5761 caagctgctg ctgaactgcg gctacaacgc gatctcggcg atcaccgaca tcccgtacgg
     5821 ccgcatggtg gcgctgcccg gtgtcgcgcg caccgtgcac gacgcagtgg ccgaatgcct
     5881 ggccgtggcc gcggcgatgg acgtgtcgat ccccggcgac gtctgggccg ccgtcgaacg
     5941 gatcgccacc agcatgcccg gccaggtctc gtccacctgc caggacctgc gccgcggcaa
     6001 gcccaccgag atcgaccacc tcaacggcta tgtcgtgcgc cagggcgacc ggctgggcat
     6061 ccccgtgccg ctgaaccggg cgctgcatgc gctggtcaag gcgctggagg cgaaggcgcg
     6121 ctgaggggcg tcggtggcgg cgagcggcgc gtgaggcctg ccacgcgaac agcaaccggc
     6181 cggtgcgccg acgatgcccg gcgcgcagcc caccgcgatg agggcgctgg cccgcgccgg
     6241 tcgggcccgc tgcgccgaag cggtcggcgc agggacgggg cgcgacgttc agcgcagcgc
     6301 cggcgccagc cgcgagaacg gatcgtccga gggcgagatc gtcgccgcgg gcttgggcct
     6361 ggaggccgcg ggggccggcg gcggtggcgg ggccggttgc aggatcgtcg cgccggggct
     6421 cgtcggctgc atcggcgtcg cgtcgatggc ggcctgcgac gcggcgtcgc gcggcgcgcc
     6481 ggccaccatc accgatggcg ggttgccggg ctccatgccg aggtaggcga tgtagccgcc
     6541 gccggcgaac tcgtcgtaga gccagtcgcc ctggacgtcc tgcacgcccg ccggctgctg
     6601 catcgtcgtg acgggcacgt ccttgagcgc cgagcgcatg aagtcgatcc agatcggcag
     6661 tgccagcgtg gcgccgaatt cgcggccgcc caggctggtg ttgtcgtcgc ggcccatcca
     6721 gaccaccgtc gtgatcctgg gctggaagcc ggcgaaccag gtgtcgacgg cgtcgttggt
     6781 ggtgccggtc ttgccgtaca ggtcggggcg cttgagcgtg gactgcgcct tggcggcggt
     6841 gccggtgcgc gccacctcgt tgagcaggct ggccatcacg aaggcgttgc gcgccgggat
     6901 gatgcgggcg tcctcggacg gcgtgtccgg cagcttgggc gcctcgaaca gcaccttgcc
     6961 ctgcgcgtcg gtgatcttct cgatgaagcg cggcgtcacg ccgaagccgc cgttggcgaa
     7021 cgcggcgtag gcgcgcgcct gctgcatcgg cgtgacgctg cccgcgccca gcgccagggt
     7081 gaggttgtcc ggctgcttct cgggcgacag gccgaactgc gcggcccagt tcttgacgac
     7141 gctggtgccc aggttctgca cgacgcggat gctgaccagg ttcttggact tggccagcgc
     7201 cgcgcgcagc gagatcgggc ccatcagcgg ctcgtcgtcg tccttcggat cccagtgctt
     7261 gccgccggtc agcgagtcgt cgatgttgat cggcgcatcc atgatctggg tggcgggctg
     7321 gatgccatgt tccagcgcgg ccgagtagat gaacggcttg aagctggaac cgggctggcg
     7381 ccaggcgctg gtcacgtggt tgaactggct gtgcgagaag tcgaagccgc ccaccagcgc
     7441 gcgcacctgg ccggtggccg agtccagcga caccagcgcg ccctcgacgt ccggccattg
     7501 cgtcaggcgc cattcgctct tgtagctgga gtcgccggtc gtgcgcatca cgcggatgat
     7561 ggcgccgcgg gtgatcgcga cgtcctcggc cgccttcggg cgcagcgcgg ccagcacgcc
     7621 gacgccgcgc agcgcgtcgc cggtcaggtg cagcgtctcg ccgctggcca ggatcgccac
     7681 cacctccttg gggctggcgt gcgtgacgac ggccacgcgc aggtcgtcgt cgtccgggtg
     7741 gtcgtgcagc gcggccgctg cggcggtgtc gatgtcggcg tcggagtcgg gcaggtcctc
     7801 gttgtcctcg gggccgcgat aggcctggcg gcggtcgaag tcgatcacgc ccttgcgcac
     7861 ggcggcctgc gccgcggcct ggtcggccga caccagcgag gtggtgacct tcaggccctg
     7921 cgagtacgcc gcctcgccga agcgctgcac cacctgctgg cgcaccatct cggccaggta
     7981 ttcggcgctc accggcggcg cccagtggcc ggtgatcttc agcggctgct tctttgcggt
     8041 ttcgtactgc gcgtcgtcga ggacgccggt ctcgtgcatg cgcatcagca cgacgagctg
     8101 gcgcgccttg gccttctcga agtggtcggt cggatcggcg ccgccggggt tcttgggcag
     8161 gcccgcgagc atcgcggcct cggccggcgt gagctgcctg gacggcttgc cgaagtaggt
     8221 cagcgacgcc gcctcgaagc cgtacgcgtg ctggcccagg tagatctggt tcatgtacag
     8281 ctcgaggatc tggtccttcg acagatgccg ctcgatcttg agcgccagca gcgcctcggc
     8341 gaacttgcgc gaccaggtct gcttgcggct caggaagaag ttgcgcgcca cctgctgcgt
     8401 gatcgtggac gcgccctggc gatggtggtg gaacaggttg g
//
LOCUS       DATCRO010000886         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_493656, whole genome shotgun sequence.
ACCESSION   DATCRO010000886 DATCRO010000000
VERSION     DATCRO010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493656"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VIN75_24105"
     CDS             complement(<1..912)
                     /locus_tag="VIN75_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY8977318.1"
                     /translation="MTVDLEQDLRAPAPRGDAPAPALSTLCAPGAATFAAATLAACGG
                     GGSSPTPSPSPSPEPPPPPPPPPAPTEAEAARFLAQASMGASRAEIASVVSLGYAGWL
                     DAQFALPVSDTRWDWLVAQGYTAFTHKNDQAGFDAAMWRKLLSAPDTLRQRITLALSE
                     IWVVAIDGLVGGGWKQFNAAAWTDMLEANAFGSHRAWLTHMSLSGAMGDFLTFRGSAK
                     EDPTSGALPDENYARELMQLFTIGLVMLDADGTPSLAGGQQVPTYGLPDITGLARVFT
                     GWDFDLQGQNVGTGTATPDFLKRPMIQV"
     gene            1228..3369
                     /locus_tag="VIN75_24110"
     CDS             1228..3369
                     /locus_tag="VIN75_24110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8977319.1"
                     /translation="MHPLPKSLRHLLVASSLPALAAVAHAADAPPPNPLLVESTLPLH
                     YPRFDQIHDSDFAPAFEQAIAEHRKEIAAIAGNPDAPTFDNTIVALERSGRTLERVNH
                     IFSNLTSANTNPALQVVQRAMAPKLAAHEDEIRLDPRLFARIDKLYDKRDTLGLDAES
                     ARLLWRYHQDFVRAGAKLPEASKASLRALNGQIASLMITFEQNTLKERGASAVTFDTR
                     AELAGLADAQIESAADAAAKAGKPGKFVIELVNTTGQPVLSQLTSHESRMKVMAASLA
                     RGSRGGEFDNRATVAALARLRAERAALLGYPDHAAFQLAEQTVGTVDTMNKLLAQLSA
                     PAVINAHKEAAQLQQMIDADHGGFQLQAADWDFYTDRLRKARYAYDESQLKPYYELDH
                     VLFDGVFYAANKLYGLTFKERHDLPVYDPGVRVFDVFDKDGTQLAIFIGDYYARANKQ
                     GGAWMNEYVPQNGLTGDKPVAANHLNIPKPPAGEPTLLTQDEVTTAFHEFGHALHGML
                     SHVKYPRFASPGVPRDFVEYPSQFNEMWATYPEVLKHFARHWKTGEPIPQALVDKVQA
                     ASLFNQGFATTEYLSATLLDQAWHQLPADKVPAAADVTTFEAAALHRAGLDFAPVPPR
                     YRSTYFSHVFANGYSAGYYSYIWSEVLAADSTDWVLAHGGLTREVGDHIRATLLSRGG
                     SVDAMTEFRDLTGGAPDVGPLLKKRGLTATE"
     gene            complement(3409..3537)
                     /locus_tag="VIN75_24115"
     CDS             complement(3409..3537)
                     /locus_tag="VIN75_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977320.1"
                     /translation="MSFTKLVAVACVLSMFAVAAEARPRHHHHHHHHHHHHHKAQR"
     gene            complement(3684..3995)
                     /locus_tag="VIN75_24120"
     CDS             complement(3684..3995)
                     /locus_tag="VIN75_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977321.1"
                     /translation="MNKTLTALLAACAATLSLGAHADDTMTHDQAEAAKAQAKADYKA
                     DKAQAKADYRANKIACERHTEGAVERACNRDAKAQAKVDKADAKLDYRQDKADIDAGR
                     K"
BASE COUNT          657 a         1549 c         1528 g          626 t
ORIGIN      
        1 gacctggatc atcgggcgct tcaggaagtc gggcgtcgcg gtgccggtgc ccacgttctg
       61 gccctgcagg tcgaagtccc agccggtgaa cacgcgtgcc aggccggtga tgtcgggcag
      121 gccgtaggta ggcacctgct ggccgccggc cagcgacggc gtgccgtcgg cgtcgagcat
      181 gacgagcccg atcgtgaaca actgcatgag ctcgcgggcg tagttctcgt cgggcagcgc
      241 gccgctggtc ggatcctcct tggcggaccc gcggaaggtg aggaagtcgc ccatcgcgcc
      301 ggacagcgac atgtgcgtga gccacgcgcg atggctgccg aacgcgttgg cctcgagcat
      361 gtcggtccag gccgcggcgt tgaactgctt ccagccgccg ccgaccaggc cgtcgatggc
      421 cacgacccag atctccgaca gcgccagcgt gatgcgctgg cgcagcgtgt cgggcgcgct
      481 gagcagcttg cgccacatcg ccgcgtcgaa gccggcctgg tcgttcttgt gcgtgaaggc
      541 cgtgtagccc tgcgcgacga gccagtccca gcgcgtgtcg gacaccggca gcgcgaactg
      601 cgcgtcgagc cagccggcgt agcccaggct cacgacgctc gcgatctcgg cgcggctcgc
      661 gcccatcgac gcctgcgcga ggaaacgcgc ggcctcggcc tcggtgggcg cgggcggcgg
      721 cggtggcggg ggcggaggtt cgggcgacgg agagggcgac ggcgtcggcg acgagccgcc
      781 accgccgcag gccgccagcg tcgcggcggc gaacgtcgcc gcacccggcg cgcagagcgt
      841 ggacagcgcc ggcgcgggcg catcgccgcg tggcgcgggc gcacgcaggt cttgctcaag
      901 atcgacagtc atggctgccc tcgttttcgg gacgtggtgt gtgtgtgtcg cggtcggtgc
      961 atcgttgaaa cgaagtcgcc gccgaggtgg ccaatgtcat cctgaaatgg ccgggttgcc
     1021 aacaagcttt gcacggagaa acaccggacg cacgatcagc aaagtcgcgc cgggccggcg
     1081 ctcgacccct tcgatccgcc attcgtcgca tggcaggccg cgccttcgct gggcgggccc
     1141 gcgactcgat agacttggcc cgccccgcgc agcaagcgcg ccggcagacc cgtcaccgcg
     1201 cgactcgcgc cccgcaccag gccgcccatg catcccctgc ccaagtccct gcgccacctg
     1261 ctcgtggctt cctccctgcc tgccctcgcg gccgtcgcgc acgccgcgga cgcgccgccg
     1321 ccgaacccgc tgctggtcga gagcacgctg ccgctgcact acccgcgctt cgaccagatc
     1381 cacgacagcg acttcgcgcc ggccttcgag caggcgatcg ccgagcaccg caaggagatc
     1441 gccgcgatcg ccggcaatcc ggacgcgccg accttcgaca acaccatcgt ggcgctggag
     1501 cgctcgggcc gcacgctgga gcgcgtgaac cacatcttca gcaacctgac cagcgccaac
     1561 accaaccccg cgctccaggt ggtgcagcgc gccatggcgc cgaagctggc cgcgcacgag
     1621 gacgagatcc gcctcgaccc gcgcctgttc gcgcgcatcg acaagctcta cgacaagcgc
     1681 gacacgctgg gactcgatgc cgagtcggcg cgcctgctgt ggcgttatca ccaggacttc
     1741 gtgcgcgccg gcgcgaagct gcccgaggcc agcaaggcca gcctgcgcgc gctcaacggc
     1801 cagatcgcgt cgctgatgat caccttcgag cagaacacgc tcaaggagcg cggcgcctcg
     1861 gcggtgacct tcgacacccg cgccgagctc gcgggcctgg ccgacgccca gatcgagtcc
     1921 gccgccgacg ccgcggccaa ggccggcaag ccgggcaagt tcgtcatcga gctggtcaac
     1981 accaccggcc agcccgtgct ctcgcagctg accagccacg agtcgcgcat gaaggtgatg
     2041 gccgcctcgc tcgcgcgcgg cagccgcggc ggcgagttcg acaaccgcgc cacggtcgcc
     2101 gcgctggcgc gcctgcgcgc ggagcgcgcg gcgctgctgg gctaccccga ccacgccgcg
     2161 ttccagctcg ccgagcagac ggtgggcacg gtcgacacca tgaacaagct gctcgcgcag
     2221 ctgtcggcgc cggccgtcat caatgcgcac aaggaggcgg cgcagctgca gcagatgatc
     2281 gacgccgacc acggcggctt ccagctgcag gccgccgact gggacttcta caccgaccgg
     2341 ctgcgcaagg ccaggtatgc gtacgacgag tcgcagctca agccctacta cgaactcgat
     2401 cacgtgctgt tcgacggcgt gttctacgcg gccaacaagc tgtacggcct gacgttcaag
     2461 gagcgccacg acctgccggt ctacgacccc ggcgtgcgcg tgttcgacgt gttcgacaag
     2521 gacggcacgc agctggcgat cttcatcggc gactactacg cgcgcgccaa caagcagggc
     2581 ggcgcgtgga tgaacgagta cgtgccgcag aacggcctga ccggcgacaa gcccgtcgcg
     2641 gccaaccacc tgaacatccc caagccgccc gccggcgagc ccacgctgct gacgcaggac
     2701 gaggtcacca ccgcgttcca cgagttcggc cacgcgctgc acggcatgct gtcgcacgtg
     2761 aagtacccgc gcttcgccag tcccggcgtg ccgcgcgact tcgtcgagta cccgtcgcag
     2821 ttcaacgaga tgtgggccac ctatcccgag gtgctcaagc acttcgcgcg ccactggaag
     2881 acgggcgagc cgatcccgca ggcgctggtg gacaaggtgc aggccgccag cctcttcaac
     2941 cagggcttcg cgacgaccga gtacctgtcg gccacgctgc tggaccaggc ctggcaccag
     3001 ctgcccgcgg acaaggtgcc ggccgccgcc gacgtcacga cgttcgaggc cgcggcgctg
     3061 catcgcgcgg ggctcgactt cgcgccggtg ccgccgcgct accgcagcac gtacttctcg
     3121 cacgtgttcg cgaacggcta ttcggccggc tactactcgt acatctggag cgaggtgctg
     3181 gcagccgact ccaccgactg ggtgctggcg cacggcggcc tcacgcgcga ggtcggcgac
     3241 cacatccgcg cgacgctgct gtcgcgcggc ggctcggtgg acgcgatgac ggagttccgc
     3301 gacctgaccg gcggcgcgcc ggacgtcggc ccgctgctca agaagcgcgg gctcaccgcc
     3361 accgagtgac ggctcgcggc cccggaggcg tccggggccg gtgtcccgtc agcgttgggc
     3421 cttgtggtgg tggtggtgat gatggtggtg gtggtgatga tggcgcggac gcgcctccgc
     3481 cgccacggcg aacatcgaca acacgcaggc gaccgcgacg agcttggtga aggacatggg
     3541 agcttctttc ttgtagggtg atggccggac tcgtgtccgg ggccggcatc atgcctcaaa
     3601 tgcggacgga ccggccccgg acgatgagca tcgtgccggc gccggccctg ccgcgatcga
     3661 tcgaagcgac ccgggttcgg gccttacttc cgcccggcgt cgatgtcggc cttgtcctgc
     3721 cggtagtcca gcttggcatc ggccttgtcc accttggcct gcgccttggc gtcacgattg
     3781 catgcgcgct cgaccgcacc ttcggtgtgg cgctcgcatg cgatcttgtt ggcgcggtag
     3841 tcggccttgg cctgggcctt gtcggccttg tagtccgcct tggcctgggc cttggcagcc
     3901 tcggcctggt catgcgtcat cgtgtcgtcg gcatgggcgc cgagcgacag cgtggcggcg
     3961 caggcggcga gcagggcggt gagggtcttg ttcatgatgg cattcccgtg atgcgcccgg
     4021 cgaggcgggc tgaggcccgg gaagcggcaa gcgcggtgcc gccgagctcg gcggtgggcc
     4081 tggatgcggc gttgtccgac agcggcgtcg agtgcctgcc ggcggcaggc cgctccgggc
     4141 cgacgtcgaa caacggccac cggcctgcaa cgccaggagc agtcaccggg catcggccga
     4201 ctgcaggatc agccgcggca ggagcccggc aggtacttca ggaggttcgg cgaagtcgcc
     4261 cccggcgcgg cggtcgagcc gcagacccac ttgtagatgt gggtgttgcc gatcacgccg
     4321 gtgccggcga ccgaatagac gctgccggtc gagtccatcg
//
LOCUS       DATCRO010000887         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_66002, whole genome shotgun sequence.
ACCESSION   DATCRO010000887 DATCRO010000000
VERSION     DATCRO010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66002"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VIN75_24125"
     CDS             <1..123
                     /locus_tag="VIN75_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8977322.1"
                     /translation="AADWGAHALDAGPRGHLNSDSGLGDWPAGRQLLANLLTSG"
     gene            266..1186
                     /locus_tag="VIN75_24130"
     CDS             266..1186
                     /locus_tag="VIN75_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8977323.1"
                     /translation="MVTKKPKGLGMGLEALLGPKIDETPAPAGGPTLPSTLKITQMQA
                     GKYQPRTRMDEGSLYELAESIRSQGIMQPILVRPLEGAGAKTQRYEIIAGERRFRAAK
                     LAGLEEVPVLVREVPDESAAVMALIENIQREDLNPLEEAQGLKRLVDEFGMTHEQAAQ
                     SVGRSRSAASNLLRLLQLAAPVQQMLMAGDLDMGHARALLPLEPAQQILNATEIAARK
                     MSVRDAEKLVARNQQANGRQAPLLRVKKEKRGDIARLEEQLADRLAAVVEIRMLKKTR
                     HGESGEIAISFGSNDELGGLLERLGLREED"
     gene            complement(1209..2780)
                     /locus_tag="VIN75_24135"
     CDS             complement(1209..2780)
                     /locus_tag="VIN75_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977324.1"
                     /translation="MIRTMRRVRLLWLALACTTLASGLAGCGGGGSAPPPPPPPPAIE
                     SAFLLAEFVAVDSNNQFVRVWDPANPSVAVQDVRIVQGNGIVWQSSHLVFSDATRWDA
                     ASRTVTTLGHAKVFFDNDGKLYSIDLRGGHAHAPVQLSSAADVFMPVRAWAMNAAGDD
                     AWVDAQGGSHDWAIRTSMTDATAPVNVRHVVAALRDASTGLPQWFFASLGDDGIHPVA
                     ATYEIADAAFARVAVPAVDGMVAADGWIGADPGQAALAYVGIAGQVRTLRWSAGGVSV
                     DAGGLHALSGLTMTTAVADATSVFVADGTTLLALAGGGVRSVGTFSVAPASLMDAGGY
                     IAADEVTQVTGVTPIVQVESLDKTSGARVLVEPASPDLQLLAGGDQGVLLSNTAGQHD
                     AYVLARGDGQARMIVGSQAVGIVRAASGRRDQPAAPVGLLSCVAGATPGYCAAGALTQ
                     FTLAGAGTSLGFNAATAAMLRSEAIAGLACSLSGQDALSSPAGFGDGQTDTRDAWQFT
                     PGTAASLTRVTSSLP"
     gene            3245..>4569
                     /locus_tag="VIN75_24140"
     CDS             3245..>4569
                     /locus_tag="VIN75_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY8977325.1"
                     /translation="MDVISQFAARYERTREEELTLEEYLAECKRNPLAYATAAERMLK
                     AIGEPQSIDTRNDPRLSRLFANKVIKIYPAFAEFYGMEDAIEQVVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSIAERLKALMEQVPFYAIKDSPVNESPLGLFNAVEDGPILESE
                     YGIPRRYLNRIMSPWAVKRLEEYGGDIRKFKVVKRRPSILRQIGISKTEPGDENNQDI
                     SSLVGKVDIRKLEAFAQDDPDAYSYSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNFKGTEGFGAIPFDGVVLAHSNESEWKSFRNNKNNEAFLDRIYIVKVPYCLRVSEE
                     VKIYEKLIRGSSLADATCAPGTLKMMAQFAMLTRLKEPENSSLYSKMQIYDGENLKDT
                     DPRAKSYQEYRDYAGVDEGMSGISTRFAYKILSKVFNFDSTEVAANPVHL"
BASE COUNT          822 a         1594 c         1545 g          608 t
ORIGIN      
        1 gccgccgact ggggcgcgca cgcgctggac gccggtccgc gcggccatct caacagcgat
       61 tccggcctgg gcgactggcc cgcggggcgg cagttgcttg cgaacctgct gaccagcgga
      121 taatcaatca tcacttccag cgcctccgcc gctggcgcta ctcaccctgc acgaagttca
      181 agggagcggc ggaaccggct ctgccgggcc gctcgcggcc ggcccctcgg gggcagggag
      241 cgccagcgac cgtggggctc acatgatggt cacgaaaaaa cccaaggggc tcggcatggg
      301 cctggaagcc ctgctcggcc cgaagatcga cgagacgccg gcgcccgcgg gcggcccgac
      361 gctgccgtcc acgctgaaga tcacgcagat gcaggccggc aagtaccagc cgcgcacccg
      421 catggacgaa ggctcgctgt acgagctggc cgagagcatc cgcagccagg gcatcatgca
      481 gccgatcctc gtgcgcccgc tcgaaggcgc gggcgcgaag acgcagcgct acgagatcat
      541 cgccggcgaa cgtcgcttcc gcgccgccaa gctggcgggc ctggaggagg tgcccgtgct
      601 ggtgcgcgag gtgcccgacg aatcggccgc cgtgatggcg ctgatcgaga acatccagcg
      661 cgaggacctc aatccgctgg aagaagcgca gggcctcaag cgcctggtcg acgagttcgg
      721 catgacgcac gagcaggccg cgcagtcggt cggccgctcg cgcagcgcgg ccagcaacct
      781 gctgcgcctg ctgcagctgg cggcgccggt gcagcagatg ctgatggcgg gcgacctcga
      841 catgggccac gcccgtgcgc tgctgccgct ggagccggcc cagcagatcc tcaacgccac
      901 cgagatcgca gccaggaaga tgtcggtgcg cgacgccgag aagctcgtcg cacgcaacca
      961 gcaggccaat ggccgccagg cgccgctgct gcgcgtgaag aaggaaaagc gcggcgacat
     1021 cgcgcgcctc gaggagcaac tggccgacag gctggccgcg gtcgtcgaga tccgcatgct
     1081 caagaagacg cgccacggcg agtccggcga gatcgcgatc tcgttcggct ccaacgacga
     1141 actgggcggc ctgctcgaac gcctgggcct gcgcgaagag gactgaaggc acggccgcgc
     1201 cgccgcgctc acggcaggct gctggtgacg cgcgtcagcg aggccgccgt gcccggcgtg
     1261 aactgccacg cgtcgcgcgt gtcggtctgc ccgtcgccga agcctgccgg cgacgagagc
     1321 gcatcctgcc ccgacagcga gcaggccagc cccgcgatcg cctcgctgcg cagcatggcc
     1381 gcggtggcgg cgttgaagcc gagcgacgtg ccggccccgg ccagcgtgaa ctgcgtcaac
     1441 gcgccggccg cgcagtagcc cggcgtcgcg cccgcgacgc aggacagcag gcccaccggc
     1501 gcggcgggct ggtcgcgacg gccgctggcc gcacgcacga tgcccacggc ctgcgagccg
     1561 acgatcatgc gcgcctggcc atcgccgcgc gccagcacgt acgcatcgtg ctggccggcg
     1621 gtgttggaca gcagcacgcc ctggtcgccg cctgccagca gctgcaggtc cggcgaggcg
     1681 ggttccacga gcacgcgcgc gccgctcgtc ttgtccaagc tctccacctg cacgatcggg
     1741 gtcacgccgg tgacctgcgt gacctcgtcg gccgcgatgt agccgcccgc gtccatcagg
     1801 gacgccggcg ccaccgagaa cgtgccgacc gagcggacgc cgccgccggc gagcgcgagc
     1861 agcgtcgtgc cgtcggccac gaagacggac gtcgcgtccg cgacggcggt cgtcatggtc
     1921 aggccgctga gcgcatgcag cccgccggcg tcgacgctga ccccgcccgc gctccagcgc
     1981 agcgtgcgaa cctgcccggc aatgccgacg taggccaagg ccgcctggcc cgggtcggcg
     2041 ccgatccagc cgtcggcggc gaccatcccg tcgacggcag gcaccgccac gcgcgcgaac
     2101 gcggcgtcgg cgatctcgta ggtggcagcc accgggtgga tgccgtcgtc gccgagcgac
     2161 gcgaagaacc attgcggcaa cccggtcgag gcgtcgcgca gcgcggcgac gacatggcgc
     2221 acgttgaccg gcgccgtcgc gtccgtcatc gacgtgcgga tcgcccagtc gtggctgccg
     2281 ccctgcgcat cgacccaggc gtcgtcgccg gccgcgttca tcgcccaggc ccgcacgggc
     2341 atgaacacgt cggccgcgct ggacaactgc acaggcgcgt gcgcatggcc gccgcgcagg
     2401 tcgatggaat acaacttgcc gtcgttgtcg aagaacacct tggcatgccc cagcgtcgtg
     2461 accgtgcgcg acgcggcatc ccagcgggtg gcatcggaga agacgagatg ggaggactgc
     2521 cagacgatgc cgttgccctg cacgatccgc acgtcctgca cggcgaccga cgggttcgcc
     2581 ggatcccaga cgcgcacgaa ctggttgttg gaatcgaccg cgacgaattc ggccagcagg
     2641 aaggccgatt cgatcgcggg cggcggcgga ggcggtgggg gcgcggaacc gccgccgccg
     2701 catccggcca ggccgctcgc gagggtcgtg cacgcgagcg ccagccacag caggcgcacg
     2761 cgcctcatgg ttcgaatcat gggtcgactc cggaattggt ctgggacgcc gcgaaggtcc
     2821 ggcgggcccg gacgggcggt gtgcggtgcg cgacaagacc aaggcctcgc ggcgacgggt
     2881 gcgggcagca ccgggccggc gggcgggtct ttccgcacac tcgtggaaag aatcctacag
     2941 ggcgagttga aatgtaggtc gataaaggac aagatgaaac cggaacatca ccctattcga
     3001 ctcatcgacg cacaaggaat cgtcgcacgc tgtaccctac ggactcagag tttgaccacc
     3061 gtacgacgac tccgctgaac ggattgctct gatggcgcct tgactggccg gtacgtactt
     3121 cccgcaagaa cgttccggac aggcccggac tccatcaaaa cattccagac gcacgcgact
     3181 ggccccgacc cgatccgcat cgtgtccggt cgtttgcaac gtcacttcag cccggaggtc
     3241 agacatggac gtgatcagcc agttcgcagc ccgctacgaa cgtactcgcg aggaggaact
     3301 caccctcgag gagtacctgg cggaatgcaa gcgcaacccc ctggcctatg ccacggcggc
     3361 cgagcgcatg ctcaaggcca tcggcgaacc gcagtccatc gacacgcgca acgacccgcg
     3421 tctgtcgcgc ctgttcgcca acaaggtcat caagatctat ccggccttcg ccgagttcta
     3481 cggcatggag gacgcgatcg agcaggtcgt gtcgtacttc cgccatgccg cgcagggcct
     3541 ggaggaaaag aaacagatcc tctacctgct gggccccgtg ggcggcggca agagctccat
     3601 cgccgagcgg ctgaaggcgc tgatggagca ggtgccgttc tacgcgatca aggattcccc
     3661 ggtcaacgaa tcgccgctcg ggctgttcaa tgcggtcgag gacggcccga tcctcgagag
     3721 cgagtacggg atcccgcggc gctacctcaa ccggatcatg tcgccgtggg ccgtcaagcg
     3781 tctcgaggaa tacggcggcg acatccgcaa gttcaaggtc gtcaagcgtc gcccgtcgat
     3841 cctgcgccag atcggcatca gcaagaccga gccgggtgac gagaacaacc aggacatctc
     3901 gtcgctggtg ggcaaggtcg acatccgcaa gctcgaggcc ttcgcgcagg acgacccgga
     3961 cgcctacagc tactccggcg gcctgtgcct ggccaaccag ggcctgctcg agttcgtcga
     4021 gatgttcaag gcgccgatca aggtcctgca cccgctgctg accgccaccc aggaaggcaa
     4081 cttcaagggc acggaaggct tcggcgcgat cccgttcgac ggcgtcgtgc tcgcgcactc
     4141 caacgagagc gagtggaaga gcttccgcaa caacaagaac aacgaggcct tcctcgaccg
     4201 catctacatc gtcaaggtgc cctactgcct gcgcgtcagc gaggaagtca agatctacga
     4261 gaagctcatc cgtggctcgt cgctggccga tgccacctgc gcgccgggca cgctcaagat
     4321 gatggcccag ttcgcgatgc tcacgcgcct gaaggagccc gagaactcgt cgctgtactc
     4381 caagatgcag atctacgacg gcgagaacct gaaggacacc gacccgcgcg ccaagagcta
     4441 ccaggagtac cgcgactacg ccggcgtcga cgagggcatg agcgggatct ccacgcgctt
     4501 cgcgtacaag atcctgtcca aggtcttcaa cttcgactcg accgaggtcg ccgccaaccc
     4561 ggtgcacct
//
LOCUS       DATCRO010000888         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_820075, whole genome shotgun sequence.
ACCESSION   DATCRO010000888 DATCRO010000000
VERSION     DATCRO010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820075"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..615)
                     /locus_tag="VIN75_24145"
     CDS             complement(1..615)
                     /locus_tag="VIN75_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY8977326.1"
                     /translation="MRVAIVTFDGFNELDSFIALGLINRLRTAGVHAEIASPSAHVTS
                     MNGVTVTAQQPLEFVTDADVVLFGSGIYTRAIAENAALMDRIVLDPTRQLIGAQCSGT
                     LLLARLGLLGDAPACTDLTTKPWVIAAGVRVSDLPFTARGPIATAGGCMASAYLATWV
                     MWRAAGREATRQALHYAAPVGEKDAWVDHALKVVGDALGDAATT"
     gene            complement(754..1275)
                     /locus_tag="VIN75_24150"
     CDS             complement(754..1275)
                     /locus_tag="VIN75_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY8977327.1"
                     /translation="MPKTKKTADAKVVVASTAIDIGISAKDRAAIAGGLSRLLADTYT
                     LYLTTHNFHWNVTGPMFNTLHAMFMGQYTELWNAVDPIAERIRSLGHPAPGSYAAFGR
                     LSSLPDAPETPPKALEMVRVLVAGHEAVARTARALFPLADKAGDEPTADLLTQRLTVH
                     EQTAWMLRSLLED"
     gene            complement(1410..1664)
                     /locus_tag="VIN75_24155"
     CDS             complement(1410..1664)
                     /locus_tag="VIN75_24155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977328.1"
                     /translation="MRIQANPSPAATLPAATKSGRSPHAPAMKATPAAAAPAKPAAAA
                     KPATPAAPVAAKVATAAQAAEAAAKPETYGGTGSLLDAHA"
     gene            complement(1922..3040)
                     /locus_tag="VIN75_24160"
     CDS             complement(1922..3040)
                     /locus_tag="VIN75_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8977329.1"
                     /translation="MTSRLPRLLAVAASVAAVLLAPVARAAAPEPKVLNIYNWSDYIA
                     DDTLKNFEKETGIKVNYDNYDSNEALHAKLVAGHTGYDIVVPSAHFAKVQLEAGLFQK
                     LDKSKLTNWGNLDPALLQLMAKVDPGNQYLVDWLWGYITVGINTGKVKAALGGMPMPA
                     NPWSLIFDPKYADKLKGCGISFLDSPSEVLPIALGYVGKPAYSKNAADYAVAEAMLKK
                     IRPDVTRFSSSGYINDMVAGSVCAVLGYSGDINIARARALKEKNGTDIVAFVPQSGST
                     LFFDSMAIPADAPHPQNALLFINYILRPEVHASLTNKVFYANPNKASLKFVNKDVAAN
                     PSVFLKPEDLAKMTVPDALPQDIKRVQTRAFTAFKTGQ"
     gene            3175..>3697
                     /locus_tag="VIN75_24165"
     CDS             3175..>3697
                     /locus_tag="VIN75_24165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY8977330.1"
                     /translation="MVDLPTAARRARHLFPASCLAAALLAAVAVPLGAGAQPAAVSTG
                     TVKYDLDDVIIHPVHSLHGMVSSESALASDVGVEVLRRGGNAVDAAVATGFALAVTMP
                     NSGNIGGGGFMILHDGKTGKDTAIDFREMAPAGASRNMYLDASGNVIQDKSLFTQAAI
                     GVPGTVAGLTYALE"
BASE COUNT          517 a         1214 c         1379 g          587 t
ORIGIN      
        1 tcaggtcgtc gcggcgtcgc ccagggcgtc cccgaccacc ttgagcgcat ggtcgaccca
       61 cgcgtccttc tcgccgaccg gcgcggcgta gtgcaacgcc tgtcgcgtgg cctcgcggcc
      121 cgcggcgcgc cacatcaccc aggtggcgag gtaggccgag gccatgcagc cgccggccgt
      181 ggcgatcggg ccgcgggccg tgaacggcag gtcggacacc cgcacgcccg ccgcgatcac
      241 ccacggcttg gtggtgaggt cggtgcacgc cggcgcgtcg ccgagcaggc ccagtcgcgc
      301 cagcagcagc gtgccggagc actgcgcgcc gatcagctgg cgcgtcgggt cgagcacgat
      361 gcggtccatc agcgccgcgt tctcggcgat ggcgcgggtg tagatgccgc tgccgaacag
      421 cacgacgtcg gcatccgtca cgaactccag cggctgctgc gccgtgacgg tgacgccgtt
      481 catcgacgtg acgtgcgccg acgggctcgc gatctccgcg tgcacgccgg ccgtgcgcag
      541 ccggttgatc aggcccagcg cgatgaagga gtcgagctcg ttgaagccgt cgaacgtgac
      601 gatggcgacg cgcatcaatt gctttctgga gtcgcggagc gacgacgccc gcggcaggcc
      661 cgaccaagct cccgggcagc gagggtcgca cccgagccga tccgggctcc ccctcgggcg
      721 gcagcgacca gcgggagcgt gggggccgtc atttcagtct tcgaggagcg aacgcagcat
      781 ccaggccgtc tgctcgtgca cggtcaggcg ctgcgtgagc aggtcggcgg tgggctcgtc
      841 gccggccttg tcggccagcg ggaacagcgc gcgggcggtg cgggcgacgg cctcgtggcc
      901 ggccaccagc acgcgcacca tctcgagcgc cttgggcggc gtctcgggcg cgtcgggcag
      961 cgagctcagg cggccgaacg cggcatagga accgggggcg gggtggccga gggagcggat
     1021 gcgctcggcg atcgggtcga ccgcgttcca cagctcggtg tactggccca tgaacatcgc
     1081 gtgcagcgtg ttgaacatcg ggccggtgac gttccagtgg aagttgtgcg tcgtcaggta
     1141 cagcgtgtag gtatcggcca gcaggcgcga taggccgccg gcgatggcgg cgcgatcctt
     1201 ggcgctgatg ccgatgtcga tggcggtgga ggcgacgacg accttggcgt cggcggtctt
     1261 cttggtcttg ggcatgcgtt ccctcgtggt ggaacgcgca agcttaggca acgggccccg
     1321 atcgaccaat gccagtggcg aaagacccct tgccggagtc ggtcgcgggg tcggtcggcc
     1381 atgtggcgcg gcaggcgcgc cgggaatggt caggcgtgag cgtcgagcag gctgccggtg
     1441 ccgccgtagg tctcgggctt ggcggcggcc tcggcggcct gggcggcggt cgcgaccttg
     1501 gcggccacgg gcgccgccgg ggtggccggc ttggctgcgg cggcaggctt ggccggcgcg
     1561 gcggccgcgg gcgtggcctt catcgccggc gcgtgcggcg agcggccgga cttggtcgcg
     1621 gcgggcagcg tggcggcggg actggggttg gcttggatgc gcatggcggg ttcctgatgg
     1681 gtcgggcgcg atgccggcct cgcaggcatt caccgcacct tcatcgtaga caggacgccg
     1741 ccgtcctaaa gcgcgatcag cgggccggaa acccgtcaaa tcgggcccca cggtcgctga
     1801 cgctccctgc ccccgagggg ccggccgcga gcggcccggc aaagccggtt ccgctgctct
     1861 ctcgacttct tgttgatcgg agcgagcggc agcgccggca caagcgcctt cggcgccaag
     1921 atcactgacc ggtcttgaag gccgtgaacg cgcgcgtctg gacgcgcttg atgtcctgcg
     1981 gcagcgcgtc cggcacggtc atcttggcca ggtcctccgg cttcaggaag acgctcgggt
     2041 tggccgccac gtccttgttc acgaacttca gcgaggcctt gttcgggttg gcgtagaaca
     2101 ccttgttggt gagcgacgcg tgcacctcgg ggcgcaggat gtagttgatg aacagcagcg
     2161 cgttctgggg gtgcggcgcg tccgccggga tggccatcga gtcgaagaac agcgtcgagc
     2221 cgctctgggg gacgaacgcg acgatgtcgg tgccgttctt ctccttgagg gcgcgggcgc
     2281 gggcgatgtt gatgtcgccc gagtagccga gcaccgcgca caccgagccg gcgaccatgt
     2341 cgttgatgta gcccgacgac gagaagcgcg tgacgtccgg gcggatcttc ttgagcatcg
     2401 cctcggccac cgcgtagtcg gcggcgttct tgctgtacgc cggcttgccg acgtagccga
     2461 gcgcgatcgg cagcacttcc gatggcgagt cgaggaagct gatgccgcag cccttgagct
     2521 tgtcggcgta cttcgggtcg aagatcagcg accacgggtt cgcgggcatg ggcatgccgc
     2581 cgagcgccgc cttgaccttg ccggtgttga tgcccaccgt gatgtagccc cacagccagt
     2641 cgaccaggta ctggttgccc gggtcgacct tggccatcag ctgcagcagc gcggggtcga
     2701 ggttgcccca gttcgtgagc ttgctcttgt cgagcttctg gaacaggccc gcctcgagct
     2761 gcaccttcgc gaaatgcgcg ctgggcacga cgatgtcgta gccggtgtgg cccgccacca
     2821 gcttggcgtg cagcgcctcg ttggagtcgt agttgtcgta gttgaccttg atgcccgttt
     2881 ccttctcgaa gttcttcaac gtgtcgtcgg cgatgtagtc cgaccagttg tagatgttga
     2941 gcaccttggg ctccggcgcg gcggcgcggg cgacgggcgc gagcaggacg gcagcgacgc
     3001 tggccgcgac ggcgaggagg cgaggcaggc gggaggtcat gggtcggtgg ctccgggagg
     3061 ggaaagcggg aaaggccgta ttgcaccacg gccatggacg gtttggctga cgggaacggt
     3121 tcggccgact accatcgccc gcagcgccca actccacaac gaggaatccc gcccatggtt
     3181 gacttgccga ccgccgcgcg ccgcgcgcgc cacctgttcc cggcctcctg cctcgccgcc
     3241 gccctgctgg cggccgttgc cgtcccgctg ggcgccggcg cgcagccggc ggcggtgtcc
     3301 accggcaccg tcaagtacga cctcgacgac gtcatcatcc atccggtgca ttcgctgcac
     3361 ggcatggtca gctccgagag tgcgctggcc tcggacgtgg gcgtggaggt gctgcgcagg
     3421 ggcggcaacg ccgtggatgc cgcggtggcc accggcttcg cgctggccgt gacgatgccc
     3481 aactcgggca acatcggcgg cggcggcttc atgatcctgc acgacggcaa gaccggcaag
     3541 gacacggcca tcgacttccg cgagatggcg cccgcgggcg cgtcgcgcaa catgtacctc
     3601 gacgccagcg ggaacgtcat ccaggacaag tcgctgttca cgcaggccgc catcggcgtg
     3661 ccgggcaccg tggccggcct cacctacgcg ctggagc
//
LOCUS       DATCRO010000889         7588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1112130, whole genome shotgun sequence.
ACCESSION   DATCRO010000889 DATCRO010000000
VERSION     DATCRO010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112130"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIN75_24170"
     CDS             complement(<1..215)
                     /locus_tag="VIN75_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY8977331.1"
                     /translation="MSTLSKRSVLALAAVLLTGCVAGPDFRSPEPPRVGSYTGADPRR
                     LAQQAFVPEPLHAGWWKDFRVPAVNDLV"
     gene            complement(218..1174)
                     /locus_tag="VIN75_24175"
     CDS             complement(218..1174)
                     /locus_tag="VIN75_24175"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8977332.1"
                     /translation="MSPRALQQDVKPRFPAPGAVARLRIHGLVRGLAHLLILGLAGCG
                     GSAMSPSATAVAPPLAAPGLEGHVVPLETIEIGSLVSGLVTQVDCDEGQVVRAGQRCA
                     RIDARPFEAAVQREDAALRAALAREAKDGLAIAYATTVLARARELVPDGVVSQETVDA
                     DDAALAQARAQVEVDRAEIARGRAMLHAARLDAGHAQIVSPIDGVIAERRVSVGQALV
                     ATLQAPTLFVVTSGPGRLAVDAAMACSEEPPPIGTPATVVLDGRPGVSLQGRVRRVDS
                     GLRCVASPAMRAVSIGVDDPDHRLAVGLRVHVRLAPAGAAGR"
     gene            complement(1326..1745)
                     /locus_tag="VIN75_24180"
     CDS             complement(1326..1745)
                     /locus_tag="VIN75_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8977333.1"
                     /translation="MKILLAIDGSEHSLRAVEFVANAGWLQAGSELLVFTAVPSLPRR
                     IESLSDPELVQRSYFTEAEEVLAPVRKRLSTMGMPVRYDWAVGPAADAIVQAAEKGQV
                     YLVVMGSHGQGALSNLVMGSVATRVLASCTVPVLLVR"
     gene            complement(1892..2377)
                     /locus_tag="VIN75_24185"
     CDS             complement(1892..2377)
                     /locus_tag="VIN75_24185"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8977334.1"
                     /translation="MSPEALPDLTDLLSAGAMCTRIVSTVEVGTTLETAARVMRETHV
                     GCVVVVDELEGESRVAGVLTDRDITVSAVALGLDARVLRAGDLMTRDVVTVREDDPLG
                     IVLDRMRERGIRRVPVVSPTGGLVGLVSFDDLQAALFLQVHAMTEVLVAGRMNEVARR
                     A"
     gene            2777..3991
                     /locus_tag="VIN75_24190"
     CDS             2777..3991
                     /locus_tag="VIN75_24190"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8977335.1"
                     /translation="MSNNVFVGLDWAAQAHAVCIVDDQGRVLDHFDVVHDRDGLVALM
                     RRLARFGSRLPIAIERPDGVLVDALVDGGHQVVPIHPNALKACRPRYRCHGGKTDATD
                     AYILADVLRTDGHRFRALAPQSDAIKALRALVRTRDDLVATRVALGNQLRSALEASWP
                     GAACIFADVTSPIGLAFLARYPSAASARRLGERRLQAFCRQHGYSGRRSAAELLARLR
                     ASANACIAEATAGAMRIVVGSLVAVLTPLVEQIAQLTHRIEHDIDELPDGRILMSFPR
                     AGRLCAAQILSELGDVRERFATADQLAAEAGAVPVTYESGKSRGVRFRWACNHRLRRA
                     ITCLADNSRHSSSWANTVYAAARARGCDHPHAIRILARAWLRVLWRAWTSRQLYDPSL
                     HGAAARLVADGG"
     gene            complement(4003..4431)
                     /locus_tag="VIN75_24195"
     CDS             complement(4003..4431)
                     /locus_tag="VIN75_24195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977336.1"
                     /translation="MIPLAAAVTLLAAFSVGRADVGRTPEGVWYSVGGIGEEESTRLA
                     IDSASHPFSLRVAARGSGAFLADVDLRITDSGNRRVFQHHLDGPWLLIDLPPGRYEVV
                     GEHEGEVSRLDVVVPAHGRRSAVMYFPVQGEVRPFREEQP"
     gene            4651..5346
                     /locus_tag="VIN75_24200"
     CDS             4651..5346
                     /locus_tag="VIN75_24200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977337.1"
                     /translation="MDHGFRSALAGIPLALAFTLSGCAAPRDQRSATQAAQAIVHDTS
                     IALQNVLEEGDRPGLRTALHGACAVLVFPHMRGGSFLAGWSGGAGVLMVRDRDQEHWL
                     GPTFFSLGDMTIGPEVALSTWELVVVFKSCDVLGPLKEGARTFSFRTSLARQSAEDDA
                     GLAVGPDIRAFAHTQGGAVGLSLHFVELQSEERLTAASHGRALPAGQAPSGEVQQGGP
                     VWALRRTMERVTK"
     gene            complement(5445..6989)
                     /locus_tag="VIN75_24205"
     CDS             complement(5445..6989)
                     /locus_tag="VIN75_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8977338.1"
                     /translation="MPTGTEAIDSLRLIEHLARRLARGGHEVTVRETHLSWILLAGRI
                     AIKLKKPVRLPFVDFSRADTRRAACEAELRLNRRLAPALYRRVVDVCGSYDAPRFGRC
                     REPIDSVLCMRRFPDGALMSERLAAGSLQPCHLERLAVRIARLHDAAQIVRDVDDPVR
                     YSMAPVEGVIEQLRPFELARWHAEARRWLDRQRLSLAPVFRSRFAGGAVRDGHGDLHL
                     ANAVVLGDDATAFDCIEFDASLRRIDVMADVAFMTMDLEAHGRLDLAHRFLDAYMEAR
                     GDFEGLAVLRFHEAARACVRRLVQRSSPGGLAGGDTDYLGVAQRKMQVRTGRPRLAIT
                     SGPSGSGKSTIAARLVDATGAVRVRSDIERKRLGGASVSSSVGALGADLYGDAMTEHT
                     YARLARCARQSLTAGYDTIIDAAFLRSSRRDAFARLAAELDVPFTVLAFDAPAAELAR
                     RVRARLGEGADPSDADEAVLAWQLRARDPVTADEERHTLQVDTSSPVDVIALASAWQS
                     CTGLDL"
     gene            complement(7023..>7588)
                     /locus_tag="VIN75_24210"
     CDS             complement(7023..>7588)
                     /locus_tag="VIN75_24210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8977339.1"
                     /translation="PPQLPFLELQFAFQVRRSTRELLPDPLTLAQIGCLLWSAGGVNR
                     QVGNGRTAPSAQGWQEVDIYAVTAQGAWLYDALEHCLLPVAAGDLRSATGEQEFCAEA
                     PLNFVYVADYGRMADARPEQREFLAGVTAGAMAENAYLAAAAFEMGCVVRAMIDRPHM
                     GATLKLGGMQHVLLAQTFGWPRRGDAV"
BASE COUNT         1188 a         2814 c         2520 g         1066 t
ORIGIN      
        1 accaggtcgt tcaccgccgg tacccggaaa tccttccacc agccggcatg gagcggctcc
       61 ggcacgaacg cctgctgcgc gagtcgccgt ggatcggcgc cggtgtagct ccccacccgc
      121 ggaggctcgg gggacctgaa gtcgggcccg gcgacgcagc cggtcagcag cactgccgcc
      181 agtgcgagga cagatcgctt ggacagcgtc gacacgctca ccgccccgcg gctcccgcag
      241 gtgcgagcct cacgtgaacc cgcaggccga ccgccagccg atgatcgggg tcgtcaacac
      301 cgatcgagac ggctcgcatg gcgggactgg cgacgcaacg caaaccgctg tcgacgcgcc
      361 gcacacgccc ctgaagcgag acgcccgggc gcccgtcgag caccaccgtc gccggcgtcc
      421 cgatcggcgg cggctcttcc gagcacgcca tcgcggcatc gacggccagc cgccccgggc
      481 cgctggtcac gacgaacagc gtcggtgcct gcagcgtcgc caccagggcc tggccgacgc
      541 tgacgcgccg ctcggcgatc acgccatcga tcggcgagac gatctgcgca tggcccgcgt
      601 ccaggcgggc cgcgtgcagc atcgcccgtc cacgcgcgat ctcggcgcgg tcgacctcga
      661 cctgcgcgcg cgcctgggcc agcgcagcgt cgtcggcgtc gaccgtctcc tgcgacacga
      721 cgccatccgg gacgagttcg cgcgccctcg cgaggaccgt ggtcgcgtac gcgatcgcca
      781 gaccatcctt ggcttcgcgc gccagcgccg cgcgcagggc ggcgtcctcg cgctgcaccg
      841 cggcctcgaa gggacgggca tcgatccgcg cgcaacgctg cccggcacgc acgacctgtc
      901 cctcgtcgca gtcgacctgc gtgaccagcc ccgagaccag gctgccgatt tcgatcgtct
      961 ccagcggcac gacgtgccct tcgagaccag gtgcggccaa cggcggcgcg acagcggtgg
     1021 cggacggcga catggcactg ccgccgcagc ctgcgagacc gaggatcagg agatgcgcaa
     1081 gcccccggac caggccatgg atgcgcagcc gggcgaccgc gcccggcgcc gggaaacgcg
     1141 gcttgacgtc ctgttgcagg gccctcggcg acatgcctca gtcacgggtg acataccaga
     1201 cgaacagcca ggccgcgcct ccaagcacca gcgcgatcgc cgacaacgcg gcgatccacc
     1261 acacgacggt ctggacgacg ttcatggtgc acgcgaattc ctgcagggct tgcacatcga
     1321 ccgcgtcaac gaaccagcag cacggggacc gtgcacgacg ccagcacgcg cgtggccacc
     1381 gaccccatca ccaggttcga cagcgcgccc tgcccgtgcg atcccatgac caccaggtag
     1441 acctggccct tctccgccgc ctgcacgatc gcgtcggccg cgggcccgac cgcccagtcg
     1501 tagcgcaccg gcatccccat cgtcgacagg cgcttgcgca ccggcgccag gacttcctcg
     1561 gcctcggtga agtagctgcg ctgcacgagc tccggatccg acaggctctc gatccgccgc
     1621 ggcagcgacg gcacggccgt gaacaccagc aactcgctgc cggcctggag ccagccggcg
     1681 ttggcgacga attcgacggc gcgcagggaa tgctcgctgc cgtcgatggc aaggaggatc
     1741 ttcatcgggc gactccggac aggcccgctc ggggagcggc gacagtccag cgtagtgctg
     1801 cgcgccgtgc cgccgcttgc gcccgcgcaa ggcgccgagg tcgcgcatgt gtccgttgcg
     1861 cgcgatcaag tggcgcaagg ggccggtgtc gtcaggcgcg cctggccacc tcgttcatcc
     1921 gccccgcgac aagcacttcg gtcatggcat gcacctgcag gaacaacgcg gcctgcaggt
     1981 cgtcgaacga gaccagtccc acgaggccgc cggtcggcga gaccacgggg acgcggcgga
     2041 tgccgcgctc gcgcatgcga tccagcacga tgcccagcgg atcgtcctcg cgcacggtga
     2101 ccacgtcccg cgtcatcagg tcgcccgcgc gcagcaccct ggcatcgagc ccgagcgcca
     2161 ccgccgacac cgtgatgtcg cggtcggtga gcacccccgc cacccgcgat tcgccctcca
     2221 gctcatccac caccacgacg cagccgacat gggtctcgcg catgacgcgg gccgccgtct
     2281 ccagcgtcgt ccccacttcg accgtgctca cgatgcgggt gcacatggcg ccagccgaca
     2341 gcaggtcggt gaggtcaggc agcgcctcgg ggctcataag gcaacctccg ccctgcgggc
     2401 tgcggtgcga gcgcccttcg gagcggccgg gcgggcgctc ataaggcaac ctccgccctg
     2461 cgggctgcgg tgcgagcgcc cttcggagtg gccgggcggg cgctcataag gcaacctccc
     2521 gcccaatgca ccgctcgttg acaggcattg cccaggagac ggaaggtgcg agtcgacttc
     2581 cgacgctctg cggtgactgc ctcgggcgct cgcccctcga gggcctgcat gagacccgcg
     2641 gcccgcaggg tgccggaagg ccgcgacgag ccacaggatg tagcgccacg cccatcaagc
     2701 tggagcgcca atttatgagc gtcccgccga gccttccgac gccggtagtc ataccgcaac
     2761 aggcaggagt ttgtccatga gcaacaacgt cttcgtcggc ctggactggg cagcccaggc
     2821 gcacgccgtg tgcatcgtcg acgaccaggg gcgcgtgctc gaccatttcg acgtggttca
     2881 cgaccgcgac ggtctggttg cgctcatgcg ccgattggcc cggttcggct cgcgtctgcc
     2941 catcgccatc gagcgccctg acggcgttct cgtcgatgca ttggtcgatg gcggccacca
     3001 ggtggtgccc atccatccca acgccttgaa ggcctgccgg ccgcgctacc gctgccacgg
     3061 tggcaagacc gacgccaccg atgcctacat cctggccgac gtgctgcgca ccgacggcca
     3121 tcgctttcgt gcgctggcgc cgcagtccga cgccatcaag gccctgcgcg cgctggtgcg
     3181 cacgcgcgac gacctggtgg ccacgcgcgt ggcgctgggc aaccaactgc gtagcgcgct
     3241 cgaggcctcg tggcccggcg ccgcctgcat cttcgccgac gtgacctcgc cgatcggcct
     3301 ggccttcctc gcgcgctatc cgtctgccgc ttcggcccgt cgtcttggcg agcgccgcct
     3361 gcaagcgttc tgtcgccagc acggctacag cggccggcgc agcgccgccg aactcctcgc
     3421 gcgcctgcgt gccagtgcca acgcctgcat cgcagaggcc actgccggcg ccatgcgcat
     3481 cgtcgttggc tcgctcgtgg ccgtgctcac gcctttggtc gagcagatcg cccagctcac
     3541 ccatcgcatc gaacatgaca tcgacgagtt gcccgacggt cgcatcctga tgtcgtttcc
     3601 acgcgccgga cgcttgtgcg ccgcacagat cctgtccgag ctgggcgacg tgcgcgagcg
     3661 ctttgccacg gccgaccaac tcgccgccga ggccggcgcc gtgcccgtga cctacgagtc
     3721 gggcaagtcg cgcggcgtgc gctttcgttg ggcctgcaac caccgtctgc gccgcgcgat
     3781 cacttgcctg gcagacaact cgcgtcactc cagctcctgg gccaatacgg tctatgccgc
     3841 tgcccgcgcc cgcggctgcg accatcctca cgccattcgt atcctggctc gcgcctggtt
     3901 gcgcgtactc tggcgcgcct ggacgagtcg tcagctctac gacccgtcgc tccacggcgc
     3961 cgctgcacgg cttgttgccg acggaggttg acacaggatg tctcatggct gctcctcgcg
     4021 gaaggggcgc acctcgccct gcaccggaaa gtacatgacg gccgagcgcc ggccgtgcgc
     4081 gggcaccacg acgtccagcc gggacacctc gccctcgtgt tcgcccacga cctcgtagcg
     4141 gccgggcggc aggtcgatca gcagccacgg gccgtcgaga tggtgctgga agactcgccg
     4201 gttgccgctg tcggtgatgc gcaggtccac gtcggccagg aacgccccgg aaccccgcgc
     4261 cgcgacccgc agcgagaacg ggtggctggc actgtcgatc gcgaggcgcg tcgactcctc
     4321 ttcgccgatg ccgcccaccg aataccagac accttccggc gtgcggccca cgtcggcacg
     4381 accgacggaa aacgccgcaa gcagggtcac cgccgcggca agggggatca cgaatcgctg
     4441 acgcatcgct cgctcctgtg catggccacc atgcgtccag tctggcgccg cgccgccctc
     4501 gcgcgttgat cgcgcgcaag gccgcggcgc gggccctcgt cggccatggc caggatcgcg
     4561 gccgaccact gatcggcctc aacacgaagc cgctccaggc cgccaacact tgtcatgaca
     4621 cgatgcggcc gatgccgcgg gagaagtgac atggaccatg gcttccgctc cgccctggcg
     4681 ggcatcccgc tggccctggc attcacgttg tcaggctgcg ctgcgccgcg cgaccaacga
     4741 tccgccacgc aggccgcaca ggcgatcgtg cacgacacca gcatcgccct gcagaacgtc
     4801 ctcgaagagg gagatcgtcc ggggctgcga accgcgctgc acggcgcctg cgccgtcctc
     4861 gtcttcccgc acatgcgcgg gggaagcttc cttgccggct ggtcgggcgg cgcgggcgtc
     4921 ctgatggtgc gcgacaggga ccaggagcac tggctcggcc cgacattctt ctcgctgggc
     4981 gacatgacca tcggcccgga ggtggcgctg tcgacatggg aactcgtcgt cgtcttcaag
     5041 agctgcgacg tgctggggcc cttgaaggaa ggcgcccgga cgttctcgtt caggacctcg
     5101 ctggccaggc agtcggcgga agacgatgcg ggcctggccg tcgggccgga catccgcgca
     5161 ttcgcacaca cgcaaggcgg agccgtcggc ctgtccctgc acttcgtcga gctccagtcg
     5221 gaagaacgcc tgaccgcggc cagccatggg cgcgcgctgc cggccggaca ggcgccttcg
     5281 ggcgaggtcc agcaaggcgg ccccgtctgg gcgctgcgcc ggacgatgga acgcgtgacc
     5341 aagtaagccg cgggcccccg gcgattcgct ggccgtctgc agcggtcgtc gagtcgatga
     5401 cgtgccggca atggcagggg tgggcaggca gtcaacgtcc gggctcacaa gtcaagcccg
     5461 gtgcacgact gccatgcgga tgcaagcgcg atcacatcga ccggcgagga ggtatcgacc
     5521 tggagggtgt gtcgttcctc gtccgcggtg accggatccc gcgcacgcag ttgccacgcg
     5581 aggaccgctt cgtcggcgtc cgatggatcg gcgccctccc cgagcctcgc ccgcacgcgc
     5641 cgggcgagct ccgctgcggg ggcatcgaac gccagcaccg tgaatggcac gtcgagctcg
     5701 gcggccaagc gcgcgaaggc gtcgcggcgg ctgctgcgca ggaaggcggc atcgatgatc
     5761 gtgtcgtatc cggcggtcag cgactgccgc gcgcaccttg caaggcgtgc gtacgtgtgt
     5821 tccgtcatgg catcgccata gagatccgcg cccagcgcgc ccaccgatga agacaccgat
     5881 gccccgccca ggcgcttgcg ctcgatgtcc gacctcaccc tcacggcgcc cgtggcatcg
     5941 accagccgcg ccgcgatcgt cgacttgccc gaaccggacg ggccgctcgt gatcgccagt
     6001 cgcggacggc ctgtgcgcac ttgcatcttc cgctgcgcga ccccgagata gtcggtgtcg
     6061 ccgccagcca atccgccggg gctcgatcgc tgcacgaggc gacgcacgca ggcccgcgcc
     6121 gcctcgtgga agcgcagcac ggcaaggccc tcgaaatcgc cgcgtgcctc catgtaggca
     6181 tcgaggaaac ggtgcgccag atcgagacgt ccgtgcgcct ccaggtccat cgtcatgaac
     6241 gcgacatccg ccatgacgtc gatgcgccgc agcgatgcgt cgaactcgat gcaatcgaac
     6301 gccgtcgcgt catcgcccag cacgacggcg ttggccaggt ggaggtcgcc atggccgtcc
     6361 cgcaccgcgc ccccggcgaa ccggcttcgg aacaccggtg caagcgacag gcgttggcgg
     6421 tcgagccatc gccgcgcttc ggcgtgccat cgcgccaact cgaagggcct caactgctcg
     6481 atcacgcctt cgaccggggc catcgaatac cgcacgggat catcaacgtc acgcacgatc
     6541 tgcgccgcgt cgtgcaaccg ggcgatgcga accgccagcc gttcaagatg gcatggctgc
     6601 aggctgcctg cggcaaggcg ctccgacatc agggcgccgt ccggaaagcg gcgcatgcac
     6661 agcacgctgt cgatcggctc gcgacagcgt ccgaagcgcg gggcgtcgta cgagccacaa
     6721 acgtcgacga cccggcggta cagagcgggt gccagccggc gattcaggcg cagctcggct
     6781 tcgcaggctg cgcgccgcgt gtccgctctc gagaagtcga cgaacggcag gcggaccggc
     6841 ttcttgagct tgatcgcgat gcgcccagcc aggaggatcc aggaaaggtg agtctcgcgc
     6901 accgtcacct cgtggccgcc gcgagcaagc cggcgggcaa gatgctcgat caggcgcagg
     6961 ctgtcgatgg cctcggttcc cgttggcacg ccggcacgct ccacaggccc acggtcgcgc
     7021 cgctacacgg cgtcgccgcg gcgcggccag ccgaaggtct gcgccagcag gacgtgctgc
     7081 atgccgccca gcttcagcgt ggcccccatg tgcggacgat cgatcatggc gcgcacgacg
     7141 caccccatct cgaacgcggc cgccgcaagg taggcgttct ccgccatcgc gcccgccgtc
     7201 acgcctgcga ggaattcccg ctgttccggc cgggcatccg ccattcgccc gtagtccgcg
     7261 acatagacga agttcaacgg ggcttcggcg caaaattcct gttctccggt ggccgaacgc
     7321 aagtcccccg ccgccaccgg cagcaggcaa tgctcgagcg cgtcgtacag ccatgcgcct
     7381 tgcgcggtca cggcatagat gtcgacctcc tgccaacctt gggccgaggg cgccgtgcgc
     7441 ccgttgccga cctggcggtt cacgccgccc gccgaccaca gcaggcaacc gatctgcgcg
     7501 agggtcaacg gatcgggcag caactcccgc gtcgaccgtc ggacctggaa cgcgaactgc
     7561 aattcgagga agggcaactg cggcgggg
//
LOCUS       DATCRO010000890         7866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_201426, whole genome shotgun sequence.
ACCESSION   DATCRO010000890 DATCRO010000000
VERSION     DATCRO010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201426"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VIN75_24215"
     CDS             <1..637
                     /locus_tag="VIN75_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977340.1"
                     /translation="DKPLVGAFAGAAATAPYYLATVLALTPLTFLAGPVVQFFQPKAI
                     AQIAAQALSPALVRKFLFGILASAFLPGLALWFAAPAVTALWLHGGGEHALVASYVRI
                     LVVGTSLGAIGYLPNVLLVARQQYRFLATASSGLTIAVLALTAWAASGGDVRTVCYVY
                     ATYHVAAAVVQWVRALTLDPELRAALSPVTPAALGASLGTVGGGLLIHRFL"
     gene            656..1696
                     /locus_tag="VIN75_24220"
     CDS             656..1696
                     /locus_tag="VIN75_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY8977341.1"
                     /translation="MTQTTFKDKTVMITGGTGSFGNTVLNRFLDTDVRQIVIFSRDEK
                     KQEDMRIALRSPKVRFVIGDTRSQESITQAMRGVDYVFHAAALKQVPSCEFYPMEAVR
                     TNVIGTENVINAAIANGVKRVVLLSTDKAVYPINAMGISKAMAEKVLIARSRGIEDGG
                     PVLCATRYGNVMASRGSVIPLFIERVRAGQALGITDPAMTRFLMSLEDSVDLVLHAFE
                     HGAQGDIFVQKAPASTIGDLAVAVSELFDAPLKTKIIGTRHGEKLYESLVSREEMAKA
                     EDMGRYWRVPADNRDLNYELYITEGSPEANAVDDYTSHNTTRLSVPQIKELLLTLSEV
                     REQLASHRAGRS"
     gene            1711..2874
                     /locus_tag="VIN75_24225"
     CDS             1711..2874
                     /locus_tag="VIN75_24225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8977342.1"
                     /translation="MNRPLLSIVVPSCNRPGTLLSVLATLVAETSAELVVSDNSDAPL
                     PNEALQALGAGERLRYRHWTERLSVVDNFERGLAMATGEYLLFIGDDDCVGPGIDAIA
                     AWMKREGIDALVSYRSRFIASYFWPGVKSRYFGDGYQGKLFLSPFSGKAILIDKRAAL
                     AGAANRPGTGLNDMARAYHGIVSRALVDRVAARFGQLFGGVSPDIYSASLLTHLADKP
                     YILDYPFVVPGASAASTAGEGAARQDTDRLDAREHIKRFGANLKWDERIPSFYSPDTV
                     WAFSHQSALDRLGVPGLGLNFPRLYLRCALMHPHHRAETLAALRVWRRTNSATRLVLG
                     VAGGFFAEFAAQVRRVWFRFVTPPKAHAGLATIGDAYATMRGQLEPWTAPDAS"
     gene            2871..3986
                     /locus_tag="VIN75_24230"
     CDS             2871..3986
                     /locus_tag="VIN75_24230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8977343.1"
                     /translation="MNAEAAGRPTKTIVISGVNLVEGGPLTVFRDCIAAATQALPGWR
                     VVVLAHDAKLITTPGVEVMAFPKAKSRWLRRLALEWHGMRPLSRELRADLWLCMHDMT
                     ARVDARRQAVYCHNPSPFSRATWRDALFSPTYFAFSLLYRYLYGAFIHRNHAVVVQQE
                     WLRRCFQRLYGVERVIVAHPQPEVAQAGVKPARQGPGNVFFFPALPRSFKNFEVIGEA
                     VALLEQDPAWQGKVRWTLSGDENRYAAWLRRRFGHLRSIEWIGLQTREQMQAQYDAVD
                     CLLFPSRVETWGLPLSEGKRHGLPLIAADLPYAHETVGNCDAVAYFDVQDPADLARRM
                     AAFENGEQPCKPARLDPPAAPFAPDWIALLKLLAEGL"
     gene            complement(3988..5268)
                     /locus_tag="VIN75_24235"
     CDS             complement(3988..5268)
                     /locus_tag="VIN75_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977344.1"
                     /translation="MASLSASHPLLQRWRAPVAMLRRARHAARTLRFNVFLLTFLPSL
                     IFFFDLSSSVSLGTSVAAAILIHGGLSVPGRQVVGLSPAGWVLAVLGVLAVHAAVAIA
                     LTPSFDVVRAGGSLLLVVLVVLAGVAFGRLLLTADDRQLHRALILCFNWLCVCAILPK
                     VGLSIADWIRPGRFSKPMFPFTEPSHFALVFMPFLLYRCVTARDSHKVLWLAGSLGIA
                     LVVQNLTLLVGSLLVGIVSLRVRSLLLWGLVAVALASTLDLSYFQSRVDVSDDTAQKN
                     LSMLVYFQGWELMAESLVNTHGWGLGFQQLGVSTSDVDAAETIYDITGEYLNMFDGGF
                     VLSKLVSELGALGLVLVAAYLARAVRAVRDLRGRRGLSAVTFANCVIVGYLTELLVRG
                     VGYFTGTFIMLVAAFWLLAAVRSAPGRRGKSPAG"
     gene            complement(5306..7228)
                     /locus_tag="VIN75_24240"
     CDS             complement(5306..7228)
                     /locus_tag="VIN75_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY8977345.1"
                     /translation="MNPSPKTPPPTVWHRLDHFLARLRPHREPLSMVVDGLLIAFAWN
                     VTYLFRIGFDRWFEARPWYDTWVMLGVIATYLVLFALLKVPQSMWRFSGFGEVKRLTW
                     ACAGAGVACAAVVMGLQLYGVPRSVLALHPLVTLMSVCMVRIGYRMLYEHSRAQITGG
                     DKEVRRALILGAGQAAKHLMAGIHQQGWIVMGMLDDDPAKQGARIAGVPVLGPLEAVH
                     DRAVLGAATHLIIAMPGAGSRQRRRAFELASTTGLPVLTVPSATELRAGDPDLARGVR
                     DIEPEDLLGREPVKLDEQGIDLVLHGKTVLITGAGGSIGSELCRQVARYAPRRLVLVE
                     LSEFNLYTIEQELGQLFPELELVRLICDVKDLAGLRRIFSAWTPHVVFHAAAYKHVPL
                     MEQHNSWAALRNNTLGTYNAALAAGEACAERFVLISTDKAVNPTNVMGATKRAAEMVV
                     SSLVGDYPGTRFMAVRFGNVLGSSGSVIPKFKEQIERGGPITVTHPEIIRYFMTIPEA
                     ARLVIQAAAIGESGQVLVLDMGEPVKIVDLARDMIRLSGHNVGDIRIEFTGLRPGEKL
                     YEELLSDNDTTLPTSVARLRIAQLDDRVSSTDALTWLSRVTEHAATMTDDGVRDALFE
                     YVPEYREAVRHAQAGR"
     gene            complement(7244..7840)
                     /locus_tag="VIN75_24245"
     CDS             complement(7244..7840)
                     /locus_tag="VIN75_24245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY8977346.1"
                     /translation="MFKRLFDVVSATLGLLLLGVPMLLVALWIRLDSPGPVFFRQERV
                     GRHGKLFRIHKFRTMAVDAEQRGPQLTVGADARVTRVGRVLRANRLDELPQFFDVLAG
                     DMSLVGPRPDVPRYVERWPADLRERVLAVRPGITDPASLTFRDEATLLARAADPEREY
                     MDVILPRKLALAADYADHASLWTDLAVIGRSVGVLLFR"
BASE COUNT         1222 a         2842 c         2726 g         1076 t
ORIGIN      
        1 ggacaagccg ctggtggggg cgttcgccgg cgccgccgcg acggcgccct actacctcgc
       61 gacggtgctg gcgctcacgc cgctcacctt cctcgccggc cccgtggtgc agttcttcca
      121 gcccaaggcg atcgcgcaga tcgccgcgca ggcgctgtcg cccgcgctgg tgcgcaagtt
      181 cctgttcggc atcctcgcca gcgcgttcct gcccgggctg gcgctgtggt tcgcggcgcc
      241 ggccgtgacc gccctgtggc tgcacggcgg cggcgagcac gccctggtcg cgagctacgt
      301 gcgcatcctg gtggtcggca cgtcgctcgg cgccatcggc tacctgccca acgtgctgct
      361 ggtggcgcgc cagcagtacc gcttcctggc caccgcatcg agcggcctga cgatcgccgt
      421 gctggcactg accgcctggg cggcctccgg cggcgacgtg cgcaccgtct gctatgtcta
      481 cgccacgtac cacgtcgccg ccgcagtggt gcagtgggtg cgtgcgctga cgctcgaccc
      541 cgagctgcgc gccgccctct cgcccgtgac cccggccgcg ctgggcgcgt cgctcggcac
      601 ggtcggcggc gggctcctca tccaccgatt cctgtgacca cccccaggtg aaccaatgac
      661 ccagaccacc ttcaaagaca agaccgtcat gatcaccggc ggcacgggct ccttcgggaa
      721 cacggtgctc aaccgcttcc tcgacacgga cgtcaggcag atcgtcatct tctcgcgcga
      781 cgagaagaag caggaggaca tgcgcatcgc gctgcgcagc ccgaaggtca ggttcgtcat
      841 cggcgacacg cgcagccagg agtcgatcac gcaggcgatg cgcggcgtcg actacgtctt
      901 ccacgccgcg gcgctcaagc aggtgccgtc ctgcgagttc tatccgatgg aggcggtgcg
      961 caccaacgtc atcggcacgg agaacgtcat caacgcggcg atcgccaacg gcgtcaagcg
     1021 cgtcgtgctc ctgagcaccg acaaggccgt ctatccgatc aacgcgatgg gcatctccaa
     1081 ggcgatggcc gagaaggtgc tgatcgcccg ctcgcgcggc atcgaggatg ggggccccgt
     1141 gctgtgcgcc acgcgctacg gcaacgtgat ggcctcgcgc ggctcggtca tcccgctgtt
     1201 catcgagcgc gtgcgcgccg ggcaggcgct gggcatcacc gacccggcaa tgacgcgctt
     1261 cctcatgtcg ctggaggact ccgtcgacct cgtgctgcac gcgttcgagc acggcgcgca
     1321 gggcgacatc ttcgtgcaga aggcgcccgc cagcaccatc ggcgacctgg ccgtggccgt
     1381 cagcgagctg ttcgacgcac cgttgaagac caagatcatc ggcacccgcc acggcgagaa
     1441 gctctacgag tcgctcgtct cgcgcgagga gatggccaag gccgaggaca tgggccgcta
     1501 ctggcgcgtg ccggcggaca accgcgacct gaactacgag ctgtacatca ccgaaggctc
     1561 gccggaggcc aacgcggtcg acgactacac gtcgcacaac acgacccgcc tgagcgtgcc
     1621 gcagatcaag gaactgctgc tgacgctgtc cgaggtgcgc gagcagctcg ccagccaccg
     1681 ggccggccgg tcgtgagcga cggcgcgagc atgaaccgtc cgctgctgtc gatcgtcgtc
     1741 ccgtcctgca accggcccgg gacgctgctg tcggtgctgg ccacgctggt ggccgagacc
     1801 tcggcggagc tcgtcgtgag cgacaacagc gatgcaccgc tgccgaacga ggcgctgcag
     1861 gccctcggcg cgggcgagcg cctgcgctat cgccactgga cggagcggct gagcgtcgtc
     1921 gacaacttcg agcgcggcct ggccatggcc acgggcgagt acctgctgtt catcggcgac
     1981 gacgactgcg tcggtccggg catcgacgcc atcgcggcgt ggatgaagcg cgaaggcatc
     2041 gacgcgctgg tgtcgtatcg cagccgcttc atcgcgagct atttctggcc cggcgtgaag
     2101 tccaggtact ttggcgacgg ctaccagggc aagctgttcc tgtcgccgtt ctcgggcaag
     2161 gcgatcctga tcgacaagcg cgccgcgctc gcgggcgcgg ccaaccggcc gggcaccggg
     2221 ctcaacgaca tggcgcgtgc gtaccacggc atcgtcagcc gcgcgctcgt cgaccgcgtg
     2281 gccgcgcgct tcggccagct gttcggcggc gtcagccccg acatctacag cgcctcgctg
     2341 ctgacgcacc tggccgacaa gccctacatc ctcgactacc cgttcgtcgt gccgggcgcg
     2401 tccgcggcca gcacggccgg cgaaggcgcc gcgcgccagg acaccgaccg cctggacgcg
     2461 cgggagcaca tcaagcgctt cggcgccaac ctgaagtggg acgagcgcat cccgtcgttc
     2521 tactcgcccg acaccgtctg ggccttctcg caccagagcg cgctggatcg cctgggcgtg
     2581 ccgggcctgg gcctgaactt cccgcgcctg tacctgcgct gcgcgctgat gcatccgcac
     2641 caccgcgcgg agacgctggc ggcgctgcgc gtctggcggc gcacgaacag cgccacgcgg
     2701 ctggtgctgg gcgtggccgg cggcttcttc gccgagttcg ccgcgcaggt gcgccgcgtc
     2761 tggttccgct tcgtcacgcc gcccaaggcc cacgccggcc tggcgaccat cggcgacgcc
     2821 tacgcgacga tgcgcggcca gctcgagccg tggacggctc ccgacgcgtc atgaacgcgg
     2881 aggctgcggg ccggccgacg aagacgatcg tcatctccgg ggtcaacctc gtcgagggcg
     2941 gtccgctgac cgtcttcagg gactgcatcg cggccgcgac gcaggcgctg cccggctggc
     3001 gcgtcgtcgt cctcgcgcac gacgcgaagc tcatcaccac gccaggcgtg gaggtgatgg
     3061 ccttcccgaa ggcgaagtcg cgctggctgc gccgcctggc gctcgagtgg catggcatgc
     3121 ggccgctgtc gcgcgagctg cgcgcggacc tgtggctgtg catgcacgac atgaccgcgc
     3181 gcgtcgacgc ccgccgccag gcggtgtact gccacaaccc gtcgccgttc tcgcgcgcga
     3241 cgtggcgcga cgcgctgttc agcccgacgt acttcgcctt ctcgctgcta taccgctacc
     3301 tgtacggcgc gttcatccat cgcaaccacg ccgtggtcgt gcagcaggaa tggctgcgcc
     3361 gctgcttcca gcgcctgtac ggcgtcgagc gcgtgatcgt ggcgcatccg cagccggagg
     3421 tcgcgcaggc gggcgtcaag ccggcgcggc agggccccgg caacgtcttc ttctttcccg
     3481 cgctgccgcg ctcgttcaag aacttcgagg tgatcggcga agccgtcgcg ctgctcgagc
     3541 aggacccggc ctggcagggc aaggtgcgct ggacgctgtc gggcgacgag aaccgctacg
     3601 ccgcctggct caggcgccga ttcggccatc tgcgcagcat cgagtggatc ggcctgcaga
     3661 cgcgcgagca gatgcaggcg cagtacgacg cggtcgactg cctgctgttc ccgtcgcggg
     3721 tcgagacctg gggcctgccg ctcagcgagg gcaagcgcca cggcctgccg ctgatcgccg
     3781 ccgacctgcc ctacgcgcac gagaccgtgg gcaactgcga cgccgtggcc tacttcgacg
     3841 tgcaggatcc ggccgacctc gcgcgccgga tggccgcgtt cgagaacggc gagcagccct
     3901 gcaagcccgc gcgcctggat ccgcccgcgg cgccgttcgc gcccgactgg atcgcgctgc
     3961 tgaagctgct ggccgaaggg ctctgactca gcccgccggc gactttccgc ggcgccccgg
     4021 cgcgctgcgc accgccgcga gcagccagaa cgccgcgacc agcatgatga aggtgccggt
     4081 gaaatagccg acgccgcgca ccagcagctc ggtcaggtag cccacgatga cgcagttggc
     4141 gaaggtcact gccgacaggc cgcgcctgcc gcgcaggtcg cgcacggccc gcacggcccg
     4201 ggccaggtag gcggccacca gcacgaggcc cagcgcgccc agctcgctca ccagcttgga
     4261 cagcacgaag ccgccgtcga acatgttcag gtactcgccg gtgatgtcgt agatcgtctc
     4321 ggccgcgtcg acgtcgctgg tgctcacgcc cagctgctgg aaccccaggc cccagccgtg
     4381 cgtgttgacc agcgactccg ccatcagctc ccagccctgg aaatacacca gcatcgacag
     4441 gttcttctgg gccgtgtcgt ccgagacgtc cacgcgcgac tggaagtagc tcaggtccag
     4501 cgtcgacgcc agcgcgacgg ccaccagccc ccacagcagc agcgagcgca cgcgcagcga
     4561 gacgatgccc accagcagcg agccgaccag cagcgtcagg ttctgcacca ccagcgcgat
     4621 gcccagcgag cccgccagcc acaacacctt gtgcgagtcg cgcgccgtca cgcagcgata
     4681 gagcaggaac ggcatgaaca cgagcgcgaa gtgcgacggc tcggtgaacg ggaacatcgg
     4741 cttggaaaag cgccccgggc ggatccagtc ggcaatggac aggcccacct tcggcaggat
     4801 cgcgcagacg cacagccagt tgaagcacag gatgagcgcg cgatgcagct ggcggtcgtc
     4861 ggccgtgagc agcagccggc cgaacgcaac gcccgccagc accaccagca ccaccagcag
     4921 cagcgagccg cccgcacgca cgacgtcgaa cgagggcgtc agcgcgatgg cgaccgccgc
     4981 gtgcacggcc agcactccca gcaccgccag cacccagccg gccggactca gtcccacgac
     5041 ctggcgcccc ggcacgctca ggccgccatg gatcaggatc gcggcagcca ccgacgtgcc
     5101 caggctgacc gaggacgaca ggtcgaagaa gaagatcagg ctgggcagga acgtcagcag
     5161 gaagacattg aagcgcagcg tgcgcgccgc gtgccgggcg cgccgcagca tcgcgacggg
     5221 cgcgcgccag cgctgcagca gcggatggct cgcgctcagc gaggccatgg cggtcgatgc
     5281 gtgggcgccc atggggtgcg tcgcctcagc ggccggcctg cgcgtggcgg accgcctcgc
     5341 ggtattcagg cacgtactcg aacagggcgt cgcgcacgcc gtcgtcggtc atcgtggcgg
     5401 cgtgctcggt gacgcggctc agccaggtca gcgcgtcggt gctggagacg cggtcgtcca
     5461 gctgcgcgat gcgcagccgc gccacggagg tcggcagcgt ggtgtcgttg tccgacagca
     5521 gctcctcgta gagcttctcg cccgggcgca ggccggtgaa ctcgatgcgg atgtcgccga
     5581 cgttgtggcc cgacaggcgg atcatgtcgc gcgccaggtc gacgatcttg accggctcgc
     5641 ccatgtccag caccagcacc tggccgctct cgccgatcgc ggccgcctgg atgaccaggc
     5701 gcgcggcctc ggggatcgtc atgaagtagc ggatgatctc cgggtgcgtc accgtgatcg
     5761 ggccgccgcg ctcgatctgc tccttgaact tggggatcac gctgcccgac gagccgagca
     5821 cgttgccgaa acgcacggcc atgaagcgcg tgcccgggta gtctccgacc agcgaggaca
     5881 ccaccatctc ggcggcgcgc ttggtggcgc ccatcacgtt ggtcgggttc acggccttgt
     5941 cggtggagat cagcacgaag cgctcggcgc aggcctcgcc ggcggccagc gccgcgttgt
     6001 aggtgcccag cgtgttgttg cgcagcgccg cccacgagtt gtgctgctcc atcagcggca
     6061 cgtgcttgta ggccgcggcg tggaacacga cgtgcggcgt ccacgccgag aagatgcggc
     6121 gcaggccggc caggtccttc acgtcgcaga tcagccgcac gagctcgagc tcggggaaca
     6181 gctgcccgag ctcctgctcg atcgtgtaca ggttgaactc cgacagctcc accagcacca
     6241 gccgccgcgg cgcgtagcgc gcgacctggc ggcacagctc ggagccgatg ctgccgcccg
     6301 cgccggtgat cagcaccgtc ttgccgtgca gcacgaggtc gatgccctgc tcgtcgagct
     6361 tgacgggctc gcgtcccagc aggtcctcgg gctcgatgtc gcgcacgccg cgcgcgaggt
     6421 cgggatcgcc cgcgcgcaac tcggtggccg acggcaccgt cagcaccggc aggccggtgg
     6481 tgctggcaag ctcgaacgcg cgccgacgct ggcgcgagcc cgcgccgggc atcgcgatga
     6541 tcaggtgcgt ggccgcgccc agcacggcgc ggtcgtgcac cgcctccagc ggcccgagca
     6601 ccggcacgcc ggcgatgcgc gcgccctgct tggccgggtc gtcgtcgagc atgcccatga
     6661 cgatccagcc ctgctggtgg atgccggcca tcaggtgctt ggcggcctgg ccggcgccga
     6721 ggatcagcgc gcgccgcacc tccttgtcgc cgccggtgat ctgcgcccgc gagtgctcgt
     6781 agagcatgcg gtagccgatg cgcaccatgc acaccgacat cagcgtgacc agcggatgca
     6841 gcgccagcac cgagcgcggc acgccgtaca gctgcaggcc catcacaacc gcggcgcacg
     6901 ccacgccggc gcccgcgcag gcccaggtca ggcgcttgac ctcgccgaag cccgagaagc
     6961 gccacatgct ctggggcacc ttcagcagcg cgaacagcac caggtaggtg gcgatgacgc
     7021 ccagcatcac ccaggtgtcg taccagggcc gcgcctcgaa ccagcggtcg aacccgatgc
     7081 ggaacaggta ggtcacgttc cacgcgaacg cgatcaggag cccgtccacc accatcgaca
     7141 gcggctcgcg atgcggccgc aggcgcgcga ggaagtggtc caggcgatgc cagacggtgg
     7201 gcggcggggt cttcggggac gggttcacga cggtgcgggg gactcagcga aagagcagga
     7261 cgccgacgct gcggccgatc acggccaggt cggtccacag cgaggcgtgg tcggcgtagt
     7321 cggcggccag cgcgagcttg cgcggcagga tgacgtccat gtactcgcgc tcggggtcgg
     7381 cggcgcgcgc gagcagcgtg gcctcgtcgc ggaaggtgag cgacgcggga tcggtgatgc
     7441 cggggcgcac ggccagcacg cgctcgcgca ggtcggcggg ccagcgctcg acgtagcgcg
     7501 gcacgtcggg ccgcgggccg accaggctca tgtcgccggc cagcacgtcg aaaaattgcg
     7561 gcagctcgtc gagccggttc gcgcgcagca cgcgccccac gcgcgtcacg cgcgcgtcgg
     7621 cgcccaccgt cagctgcggg ccgcgctgct ctgcgtccac cgccatcgtg cggaacttgt
     7681 ggatgcggaa cagcttgccg tgccggccca cccgctcctg gcggaagaac accgggcccg
     7741 gcgagtcgag gcggatccac agcgcgacga gcagcatcgg cacgcccaag agcagcaggc
     7801 cgagggtggc cgacaccacg tcgaacagac gcttgaacat gcgacggacc ggccgcggcc
     7861 ttcagc
//
LOCUS       DATCRO010000891         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1089741, whole genome shotgun sequence.
ACCESSION   DATCRO010000891 DATCRO010000000
VERSION     DATCRO010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089741"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="purD"
                     /locus_tag="VIN75_24250"
     CDS             <1..1065
                     /gene="purD"
                     /locus_tag="VIN75_24250"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8977347.1"
                     /translation="LSQGVVDAFRSHGLRIFGPTRAAAQLESSKAFSKNFMKRHGIPT
                     AAYETFTDIAAAHAYVDRLGAPIVVKADGLAAGKGVVVAMTVDEAHAAVDWMLKPEGG
                     LAVQHNDAPRVVIEEFLVGEEASFIVMVDGKNVLPLASSQDHKRLGDNDTGPNTGGMG
                     AYSPAPVVTPNVHQRVMHEIILPTVEGMARDGIVFSGFLYAGLMIDANGHPKTLEFNT
                     RMGDPETQPIMMRLKSDLAEVLMHATDGTLDQVELQWDRRVALGVVMAAAGYPETPRK
                     GDRITGLPAEAEDAVVFHAGTALQGDVTSVTGGRVLCVTALGDSTRAAQQRAYEALRG
                     IHFDGAQHRTDIGHRAIHKR"
     gene            1103..1741
                     /locus_tag="VIN75_24255"
     CDS             1103..1741
                     /locus_tag="VIN75_24255"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8977348.1"
                     /translation="MPAPLGEARAAGPARLPVRPLEFEGSRLARAVLRLCGWRVVCDG
                     LPARQGVVIVYPHTSNWDFVWAMLAKWALGVPVTFWGKATLFRIPLMGRWLRWLGGIP
                     VVRDAPNGVVGQMAQRLRDARRRDEFMWLGLSPEGTRRRTPGWRSGFHQVALQADVPV
                     VLAYLDYPRREVGLESAWRLSGDAAADLACFAQRLQGRQGRRADQAAPVRLI"
     gene            1755..>2165
                     /locus_tag="VIN75_24260"
     CDS             1755..>2165
                     /locus_tag="VIN75_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY8977349.1"
                     /translation="MNTIPASSSAAETPDVAAVRAWLLDLQGRIVAAMEAADGGAFIS
                     DGWTRPAGGALEGDGLTRLIEGGALVERGGCNFSHVKGRTLPPSATAARPELAGAGFE
                     ALGVSLVFHPRNPYVPTVHMNVRMFCAFPKEGAAV"
BASE COUNT          297 a          791 c          781 g          296 t
ORIGIN      
        1 ctgtcgcagg gcgtcgtcga cgcgttccgc tcgcacggcc tgcgcatctt cggccccacg
       61 cgggccgcgg cgcagctcga gagctcgaag gcgttctcga agaacttcat gaagcgccac
      121 ggcatcccca ccgcggccta cgagacgttc accgacatcg ccgccgcgca cgcgtacgtc
      181 gacaggctgg gcgcgcccat cgtcgtcaag gccgacggcc tggcggcggg caagggcgtc
      241 gtcgtggcga tgaccgtcga cgaggcgcac gccgccgtcg actggatgct caagcccgaa
      301 ggcggcctgg cggtgcagca caacgacgcg ccgcgcgtcg tcatcgagga gttcctcgtt
      361 ggcgaggaag ccagcttcat cgtgatggtg gacggcaaga acgtgctgcc gctggcctcc
      421 agccaggacc acaagcgcct gggcgacaac gacaccggcc ccaacaccgg cggcatgggt
      481 gcgtactcgc cggccccggt cgtcacgccc aacgtgcacc agcgcgtgat gcacgagatc
      541 atcctgccga ccgtcgaggg catggccagg gacggcatcg tcttcagcgg cttcctgtac
      601 gccggcctga tgatcgacgc caacgggcat ccgaagacgc tcgagttcaa cacccgcatg
      661 ggcgaccccg agacgcagcc gatcatgatg cgcctcaaga gcgacctcgc cgaggtgctg
      721 atgcacgcca cggacggcac gctcgaccag gtcgagctgc aatgggaccg gcgcgtcgcg
      781 ctgggcgtcg tgatggccgc cgccggctat ccggaaaccc cgcgcaaggg cgaccgcatc
      841 acgggcctgc ccgccgaggc cgaggacgcc gtcgtgttcc atgcgggcac cgcgctgcag
      901 ggcgacgtca ccagcgtcac cggcggccgc gtgctgtgcg tgaccgcgct cggcgactcc
      961 acgcgcgccg cccagcagcg cgcgtacgag gcgctgcgcg gcatccattt cgatggcgcg
     1021 cagcatcgca ccgacatcgg ccaccgtgcc atccacaagc gctgagtgct gggacgccgg
     1081 ggcgcgcgat ttcgccgcct cgatgccggc gccgctgggc gaggcccgcg ccgccggccc
     1141 cgcgcgcctg ccggtgcggc cgctcgagtt cgaaggcagc cgcctcgccc gcgccgtgct
     1201 gcggctttgc ggctggcggg tcgtctgcga cggcctgccg gcgcggcagg gcgtcgtgat
     1261 cgtctatccg cacacgtcca actgggactt cgtctgggcc atgctggcca agtgggcgct
     1321 gggcgtgccg gtcacgttct ggggcaaggc cacgctgttc cgcatcccgc tgatgggccg
     1381 ctggctgcgc tggctgggcg gcatcccggt ggtgcgcgac gcgcccaacg gcgtcgtcgg
     1441 ccagatggcg cagcgcctgc gcgacgcgcg ccggcgcgac gagttcatgt ggctggggct
     1501 gtcgcccgaa ggcacgcgca ggcgcacgcc cggctggcgc agcggcttcc accaggtggc
     1561 gctgcaggcc gacgtgccgg tggtgctcgc ctacctggac tacccccggc gcgaggtcgg
     1621 cctcgagtcg gcctggcgcc tgagcggcga tgccgccgcg gacctggcat gcttcgcgca
     1681 gcgcctgcag ggccgccagg gccggcgcgc cgaccaggcg gcgccggtgc ggctgatctg
     1741 aactcctcga tgccatgaac acgatccccg cgtcctcatc cgcggccgag acacccgacg
     1801 tcgcggccgt ccgggcgtgg ctgctcgacc tgcagggtcg catcgtcgcc gccatggaag
     1861 cggccgatgg cggcgccttc atcagcgacg gctggacgcg tccggcgggc ggtgcgctcg
     1921 aaggcgacgg cctgacgcgg ctgatcgagg gcggggcgct cgtcgagcgc ggcggctgca
     1981 acttcagcca tgtcaagggc aggacgctgc cgccctcggc gaccgccgcg cggcccgagc
     2041 tcgccggcgc cggcttcgag gcgctgggcg tgtcgctcgt gttccatccg cgcaacccgt
     2101 acgtgcccac cgtgcacatg aacgtgcgca tgttctgcgc gttcccgaaa gaaggtgcgg
     2161 cggtg
//
LOCUS       DATCRO010000892         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_696600, whole genome shotgun sequence.
ACCESSION   DATCRO010000892 DATCRO010000000
VERSION     DATCRO010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696600"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1397)
                     /locus_tag="VIN75_24265"
     CDS             complement(84..1397)
                     /locus_tag="VIN75_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY8977350.1"
                     /translation="MPPTSAQAESNTRPATASGASGGKKGLAALVGPGLLTGASDDDP
                     SGIGTYSQVGAQFGFGMLWTMLFSYPLMVAAQLVCARIGRVTGKGLAGNLREHFPRWA
                     LWPLVLLLLVANTINIGADLGAMGEGIHLLLPGHAAWYTVAFGVLSVALEVMVPYHRY
                     VNVLKWLTLALLAYVATAFVVDVPWGRVAHDTFLPPLSLGRDYVTAIVAIFGTTISPY
                     MFFWQASQEVEELDACEEEHALRDAPQQASAQLHRIDVDTYLGMGASNVVGFFIVLTT
                     AATLHVHGIHDIQSAAQTAQALKPVAGEFAFALFAMGMVGTGLLAVPVLAGSAAYAVG
                     ETMQWKSSLESKPGRARRFYAVIAVATLAGTAMNFVHLNPIKALFWTAVINGVIAVPM
                     LAAVMLVAAREKAMGRFAISGGLRAMGWLTTAVMAACVAGMAFTA"
     gene            complement(1451..>2175)
                     /locus_tag="VIN75_24270"
     CDS             complement(1451..>2175)
                     /locus_tag="VIN75_24270"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8977351.1"
                     /translation="TPGLLDVATHHHFGWDAARDDLGAQSLRPLDDAREMPATPAQLA
                     AFVREDAATSGRYARAFGAAPGPDDARIRRDVGLALAAYVETLATPRTPFDDWRDRLA
                     ATGDATPTPAFPAAAVRGVRLFAGRAGCAACHGGPALSDDAPHVSLVHSLAAPGIPDD
                     GHPGGPVNAFRTPGLRDVAATAPYLHDGSAARLCDALRPHAADPAHPAPALTASERSD
                     LAAFLRALSAPRDDADDRACDG"
BASE COUNT          294 a          766 c          843 g          272 t
ORIGIN      
        1 gcgtgggctc cagccaccgc cggcgcctgc ccggcgccca tggtcgcggc ccggcctttc
       61 gcgaggccgg gccgctttct tcctcaggcc gtgaacgcca tgccggcgac gcaggcggcc
      121 atcaccgcgg tcgtgagcca gcccatggcg cgcaggccgc ccgagatcgc gaagcgcccc
      181 atggccttct cccgggccgc gacgagcatc accgccgcga gcatgggcac ggcgatcacc
      241 ccgttgatca cggccgtcca gaacagcgcc ttgatcgggt tcaggtgcac gaagttcatc
      301 gcggtgccgg ccagcgtggc caccgcgatc accgcataga agcgccgcgc gcgcccgggc
      361 ttgctctcga ggctgctttt ccactgcatg gtctcgccga ccgcgtaggc cgcgctgccc
      421 gcgagcaccg gcacggcgag cagcccggtg ccgaccatgc ccatcgcgaa cagcgcgaac
      481 gcgaactcgc cggccaccgg cttgagcgcc tgcgcggtct gggcggcgct ctggatgtcg
      541 tggatgccgt ggacgtgcag cgtcgcggcc gtggtcagca cgatgaagaa gccgacgacg
      601 ttggaggcgc ccatgcccag gtacgtatcg acgtcgatgc ggtgcagctg cgcgctggcc
      661 tgctggggcg cgtcgcgcag ggcgtgctcc tcctcgcagg catcgagctc ttcgacctcc
      721 tgcgaggcct gccagaagaa catgtacggg ctgatcgtgg tgccgaagat ggcgacgatg
      781 gccgtcacgt agtccctgcc gagcgacagc gggggcagga aggtgtcgtg cgcgacgcgg
      841 ccccacggga cgtcgacgac gaaggccgtc gcgacatagg ccagcagcgc gagcgtcagc
      901 cacttcagga cgttgacgta gcggtgatag ggcaccatca cctcgagcgc caccgacagc
      961 acgccgaacg ccaccgtgta ccaggccgcg tgcccgggca gcagcaggtg gatgccttcg
     1021 cccatcgcgc ccaggtcggc cccgatgttg atggtgttgg ccaccagcag cagcagcacc
     1081 agcggccaga gcgcccagcg cgggaagtgc tcgcgcaggt tgccggccag gcccttgccc
     1141 gtgacgcgcc cgatccgcgc gcacacgagc tgcgcggcca ccatcagcgg gtaggagaac
     1201 agcatcgtcc acagcatgcc gaagccgaac tgcgcgccga cctgcgagta ggtgccgatg
     1261 ccgctcgggt cgtcgtcgga cgcgccggtc agcaggcccg ggccgaccag cgcggccagt
     1321 cccttcttgc cgccggaggc gccggacgcg gtcgctggac gggtgttcga ttcggcctgg
     1381 gcggaggtgg gcggcatgcg gcggccgggc aatagccgtg ccggacgtcc ggtgccatgg
     1441 acggctcgtg tcagccgtcg caggcgcggt cgtccgcgtc gtcgcgcggc gcggacaggg
     1501 cgcgcaggaa ggcggcgaga tcgctgcgct cgctggccgt caacgccggc gccggatgcg
     1561 ccgggtcggc ggcgtgcgga cgcagcgcgt cgcacagacg ggcggcgctg ccgtcgtgca
     1621 ggtagggcgc ggtggcggcg acgtcgcgca ggccgggcgt gcggaacgcg ttcacggggc
     1681 cgcccgggtg gccgtcgtcg gggatgcccg gcgcggcgag cgagtgcacc agcgagacgt
     1741 gcggcgcgtc gtcgctgagc gccggtccgc cgtggcaggc ggcgcagccg gcgcgcccgg
     1801 cgaacagccg cacgccacgg acggcggcgg cgggaaaggc gggcgtcggc gtcgcgtcgc
     1861 ccgtggcggc gagccggtcg cgccagtcgt cgaagggtgt acgaggcgtg gccagggtct
     1921 cgacgtaggc ggccagcgcg aggccgacgt cgcgccggat gcgggcgtcg tcggggccgg
     1981 gcgccgcgcc gaatgcgcgg gcgtagcgcc ccgacgtcgc cgcgtcctcc cgcacgaagg
     2041 cggccagctg cgcgggcgtc gcaggcatct cgcgggcgtc gtccagcggc cgcaggctct
     2101 gggcgccgag gtcgtcgcgt gcggcgtccc agccgaagtg gtggtgcgtc gcgacgtcca
     2161 gcaggccggg ggtgt
//
LOCUS       DATCRO010000893         6949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_662572, whole genome shotgun sequence.
ACCESSION   DATCRO010000893 DATCRO010000000
VERSION     DATCRO010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662572"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VIN75_24275"
     CDS             complement(<1..492)
                     /locus_tag="VIN75_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8977352.1"
                     /translation="MIAATVRTRGSLAGRIGYLAAALVVMAVSVFPLLVAVSSSLKTG
                     DGLFDPSLVPAAPTLENYRHLFAEHPFGRNLLNSAIVSTGVVALSLVLSLMAAWSLGR
                     VRFRGRGTLMMCILAASMFPQVAVLSGMFELVRALGLYDSLGALIVSDLMLTLPFTVW
                     VLVT"
     gene            complement(489..1430)
                     /locus_tag="VIN75_24280"
     CDS             complement(489..1430)
                     /locus_tag="VIN75_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8977353.1"
                     /translation="MSTLDSPGWGVRRTGRRTLAASRVRQALWMLAPMLLLLAAVAGW
                     PLARTIWISLTDMQLSDPHAARFVGLDNYLGRYGLLADPAWWRAVFTTLGFALVSVSL
                     ETLLGLGVALLLNHPSRLRTALRACVLVPWAIPTVVSAKMWGWMLHDQFGVVNHTLSA
                     LHLIHGPVAWTADPTLSLLSVVAVDVWKTTPFMALLILAALQTVPGDCYEAAAVDGVP
                     PRAVFWRVTLPLIAPGVAVAVVFRLLDAMRVFDLVYVMTANSRATRTMSVYVREQLID
                     FQQVGYGSAAATVLFLVIGLSTLATLRVLRSPAEDAA"
     gene            complement(1442..2752)
                     /locus_tag="VIN75_24285"
     CDS             complement(1442..2752)
                     /locus_tag="VIN75_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8977354.1"
                     /translation="MRPFKNKHGQVKASTGRRAAALLALAAAAFLCSGARAESIKISC
                     GAVGRELEQCREAAQAWARKTGNQVQVVATPNDSSERLALYEQVLSSGSDKIDVFQVD
                     VVWPGLLGSHFIDLRPYTHGAEQQMFPDFVAADTKDGRLVAMPWFINAGLLFYRKDLL
                     AKYGLAVPETWAALAQDARRVQEAERAAGNDKMWGYVWQGRAYEGLTCDAVEWLASYG
                     GGAIVERDGRISVNNPRAAAALRTAASWVGDISPAAVLNYAEEESRGVFQAGNAVFMR
                     NWPYAWSLAQAAQSSIRGKVGVAVLPATATGPHSATLGGESLAVSRYSRYPALAADLV
                     MYMTSAAVQKQRAIGSSFNPSLLALYKDADVLAANPFMGELYPVFTHAVSRPAVSAGT
                     RYNQVSNEFWNAAHDVLSHRAKAEDALAELAATLERVSRGGKWN"
     gene            complement(2876..5578)
                     /locus_tag="VIN75_24290"
     CDS             complement(2876..5578)
                     /locus_tag="VIN75_24290"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977355.1"
                     /translation="MLMPAAGVVEPAAAAGGERPVRRLGRVRTRLLAAFLLLLGATVL
                     LTAVGWVGLRRMQSTLAGVEGDLLPRISHALELSERATQLASTAPKLGDSLSAAELDT
                     HRQVVESLLAQVGRSTSEIGETTPRLRSVIDRLQQSVHQDLPALIELSQRKLREQERL
                     DASLKRLDRLGQVLHPAHGEPADPRISSLWSTLVLGAAVSDTATIGRLEADAEALLLS
                     ARQHGATKSLAPEDARELVALTEGPDSVLGCRQALVNYDAQIDYLVTITRANADDLSD
                     ETSRYVAQLRLAAADRSERMRVAVRASEALMLVLTALCVLIGAFATRYVRRLVGEIEN
                     ITRVMSRLAAGDTALSTPATARPDELGALARSFEVFRDNLLAKQQLVRDLHAQRELLE
                     AVHDNLTDGLAVFDRDGRLLLWNNRLVEMLGRQGVSPAAGALPSSLLAAMPAGTTWSS
                     PESQRQVPVGELAPEAWLRAGHLELHAPDGQVLDLRSCSMPGGGTVSLVTDLSARRAI
                     ETQLQQAQKLETLGQLTGGVAHDFNNYLGTILGNLALLEATVGPEDERARVQWGRVRR
                     AATSAAGLTRRLLAFARRQPLEAEDVLLDEMVEEMRDLVEYSASDAVELVLELDGGDA
                     PVHIDRGQLENALLNLVMNSAAAMPRGGTLTVSTRRVHGRDGLPWIELAVADTGIGMS
                     EAQAARAFEPFFSTRPTGEGSGLGLSIVYGFVRQSGGDITLQTQPGHGTRVALRFPTT
                     APATRGVPSLATPIAALDPLPGRALLVVENDEAFGATLAEQLRQLGVRVHSATNAEDA
                     MDALAGGLHVHGLVSDIRLGQGANGIALVRMVRARWPGLPVMLMSGEVPELAVDAGGD
                     GAEGPGTGIEGLRDSHAGAPHFLQKPFPLSQLAAWIRTLQVTGV"
     gene            complement(5572..6324)
                     /locus_tag="VIN75_24295"
     CDS             complement(5572..6324)
                     /locus_tag="VIN75_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8977356.1"
                     /translation="MEAIETARPSVLVVDDEPDMVASLQDVLRRAGFEADGASNGEDA
                     RRAMAGRDYALMVLDPRLKAEDGLTLARQLRQTSAIPIIMISGSSDETDRVLLLELAA
                     DDFLIKPFSPRELIARVRAVLRRYDPAPQAPVPARAPAAAGNNERLRFGNWTLDLTER
                     ELHRADGSLCALTQAEFRLLEAFVRQPRRIWTRDQLLEHTRSLDTEVFDRTIDVLILR
                     LRRKIESNPKHPAFICTERGMGYVFAVDVSPC"
     regulatory      6729..6791
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT          997 a         2559 c         2450 g          943 t
ORIGIN      
        1 ggtgaccagc acccacacgg tgaacggcag cgtgagcatc aggtcggaca cgatcagcgc
       61 gcccaggctg tcgtacaggc ccagcgcgcg cacgagctcg aacatgcccg acagcaccgc
      121 gacctgcggg aacatcgacg cagccaggat gcacatcatc agcgtgccgc gtccgcggaa
      181 gcgcacgcgt ccgagcgacc acgccgccat caggctcagc acgaggctca gcgcgacgac
      241 gccggtcgag acgatcgccg agttcagcag gttgcgcccg aacgggtgct cggcgaacag
      301 gtggcggtag ttctccagcg tcggggcggc cggcacgagg ctcgggtcga acaggccgtc
      361 gccggtcttg agcgagctcg acacggccac cagcagcggg aacaccgaca ccgccatcac
      421 gacgagcgcg gcggccaggt agccgatgcg gcccgccagc gagccgcgcg tgcgcaccgt
      481 tgcggcgatc atgcggcgtc ttccgcgggc gagcgcagca cgcgcagcgt cgcgagcgtg
      541 ctcagcccga tgaccaggaa cagcacggtg gccgccgccg agccgtagcc gacctgctgg
      601 aagtcgatca gctgctcgcg cacgtacacc gacatcgtgc gcgtggcgcg gctgttggcc
      661 gtcatgacgt agacgaggtc gaacacgcgc atcgcgtcga gcagccggaa caccaccgcc
      721 accgccacgc ccggcgcgat cagcggcagc gtgacgcgcc agaagaccgc gcgcggcggc
      781 acgccgtcga cggccgcggc ctcgtagcag tcgccgggca ccgtctgcag cgcggccagg
      841 atcagcagcg ccatgaacgg cgtggtcttc cagacgtcga ccgccaccac cgacagcagc
      901 gacagcgtcg gatcggccgt ccacgccacc gggccgtgga tcaggtgcag cgcggacagg
      961 gtgtggttca ccacgccgaa ctggtcgtgc agcatccagc cccacatctt ggccgacacc
     1021 accgtgggga tcgcccaggg cacgagcacg caggcgcgca gggccgtgcg cagacgcgac
     1081 gggtggttca gcagcagcgc gacgcccagc cccagcagcg tctccagcga caccgacacg
     1141 agcgcgaacc cgagcgtggt gaagaccgcg cgccaccatg ccgggtcggc cagcagccca
     1201 taccgcccca ggtagttgtc cagcccgacg aagcgcgcgg catgcgggtc ggacagctgc
     1261 atgtccgtca ggctgatcca gatcgtgcgc gcgagcggcc atccggccac cgccgcgagc
     1321 agcagcagca tcggcgcgag catccacaac gcctggcgca cgcggctggc ggccagcgtg
     1381 cgccggcccg tgcggcgcac gccccatcca ggagagtcga gcgtcgacat cgatgccccg
     1441 ctcagttcca cttgccgccc cggctgacgc gctcgagcgt ggcggccagc tcggccagcg
     1501 cgtcctcggc cttggcgcga tgcgacagca cgtcgtgcgc ggcgttccag aactcgttgc
     1561 tcacctggtt gtagcgcgtg ccggcgctca ccgcgggtcg cgacacggca tgggtaaaga
     1621 ccgggtagag ctcgcccatg aacgggttgg cagccagcac gtcggcgtcc ttgtacagcg
     1681 cgagcaggct cgggttgaac gagctgccga tcgcgcgctg cttctgcacg gcggcgctgg
     1741 tcatgtacat caccaggtcg gcggcgagcg ccggatagcg cgagtacctc gacacggcca
     1801 gcgattcgcc gcccagcgtc gccgaatgcg gccccgtggc ggtggccggc agcacggcca
     1861 cgccgacctt gccgcggatg ctgctctgcg ccgcctgcgc cagcgaccac gcgtagggcc
     1921 agttgcgcat gaacaccgcg ttgcccgcct ggaacacgcc gcgcgattcc tcctcggcgt
     1981 agttcaggac ggcggccggc gagatgtcgc cgacccagct cgccgcggtg cggagcgcgg
     2041 cggccgcccg cgggttgttc accgagatgc ggccgtcgcg ctcgacgatg gcgccgccgc
     2101 cgtagctcgc gagccactcg acggcgtcgc aggtcagtcc ctcgtaggcg cggccctgcc
     2161 agacgtagcc ccacatcttg tcgttgccgg ccgcgcgctc ggcctcctgc acgcggcgcg
     2221 catcctgcgc gagcgcggcc caggtctcgg gcaccgccag gccgtacttg gccagcaggt
     2281 ccttgcggta gaacagcagg cccgcgttga tgaaccacgg catcgccacg aggcggccat
     2341 ccttggtgtc ggcggcgacg aagtccggga acatctgctg ctcggcgccg tgcgtgtagg
     2401 gccgcaggtc gatgaagtgc gagccgagca ggccgggcca gaccacgtcc acctggaaca
     2461 cgtcgatctt gtccgagccc gagctgagca cctgctcgta cagcgccagg cgctcgctcg
     2521 agtcgttggg cgtggcgacg acctgcacct ggttgcccgt cttcctcgcc cacgcctgcg
     2581 cggcctcgcg gcactgctcg agctcgcggc ccaccgcgcc gcaggagatc ttgatcgact
     2641 cggcgcgtgc gccggagcaa aggaacgcgg cggccgccag cgccagcagc gccgcggccc
     2701 gccgtcccgt cgacgccttc acctgcccgt gcttgttctt gaatggcctc attgctgtct
     2761 ccaacgtctc agtcctcgtc gagccgcctc gcgaaccccg gaactctcgt cgacgtcgga
     2821 atcgatttga ccgccgccgc gcgcgctgaa gatttcgaat gtttcgggcc gcggcctaca
     2881 cgcctgtaac ctgcagcgtg cggatccagg ccgcgagctg gctcaacggg aacggcttct
     2941 gcagaaagtg cggcgcgccg gcgtgcgagt cgcgcaggcc ctcgatgccg gtgcccggcc
     3001 cctcggcgcc gtcgcccccg gcgtccacgg ccagctcggg cacctcgccg gacatcagca
     3061 tcaccggcag ccccggccag cgcgcgcgca ccatgcgcac cagcgcgatg ccgttggcgc
     3121 cctgcccgag gcggatgtcg ctcaccaggc catggacgtg caggccgccg gccagcgcgt
     3181 ccatcgcgtc ctcggcgttc gtggccgagt gcacgcgcac gccgagctgg cgcagctgct
     3241 cggccagcgt cgcgccgaag gcctcgtcgt tctcgacgac gagcagcgca cgtcccggca
     3301 gcggatcgag cgcggcgatc ggcgtggcga gcgacggcac gccgcgggtc gcgggcgccg
     3361 tcgtgggaaa gcgcagcgcg acgcgcgtgc cgtggccggg ctgcgtctgg agcgtgatgt
     3421 cgccgcccga ctggcgcacg aagccgtaga cgatggacag gcccaggccg ctgccctcgc
     3481 ccgtgggccg cgtcgagaag aacggctcga acgcgcgcgc cgcctgcgcc tcgctcatgc
     3541 cgatgccggt gtcggccacc gcgagctcga tccacggcag gccgtcgcgg ccgtggacgc
     3601 gcctggtcga cacggtcagc gtgccgccgc gaggcatcgc ggccgcgctg ttcatcacga
     3661 ggttgagcag cgcgttctcg agctggccgc ggtcgatgtg cacgggcgcg tcgccgccgt
     3721 cgagctccag cacgagttcg accgcgtcgc tcgcgctgta ttcgacgaga tcgcgcatct
     3781 cctcgaccat ctcgtccagc agcacgtcct cggcctccag cggctggcgg cgcgcgaacg
     3841 cgagcaggcg ccgcgtgagg ccggccgcgc tggtggcggc gcgccgcacg cgtccccact
     3901 gcacgcgggc gcgttcgtcc tcgggaccga cggtggcctc cagcagcgcg aggttgccca
     3961 ggatggtgcc caggtagttg ttgaagtcat gcgcgacgcc gccggtcagc tgccccagcg
     4021 tctcgagctt ctgcgcctgc tgcagctgcg tctcgatggc gcggcgcgcg ctgaggtccg
     4081 tcaccagcga gaccgtgccg ccgccgggca tcgagcagct gcgcaggtcc agcacctggc
     4141 cgtcgggggc gtgcagctcc aggtgcccgg cacgcagcca ggcctcgggc gcgagctcgc
     4201 cgacaggcac ctggcgctgc gattccgggc tgctccaggt cgtgcccgcg ggcatcgccg
     4261 ccagcagcga cgacggcagc gcgccggccg cgggggacac gccctggcgg cccagcatct
     4321 cgacgagccg gttgttccac agcagcaggc ggccgtcgcg gtcgaacacg gccaggccgt
     4381 cggtcaggtt gtcgtgcacc gcctcgagca gctcgcgctg cgcgtgcagg tcgcgcacca
     4441 gctgctgctt ggccagcagg ttgtcgcgga acacctcgaa actgcgtgcg agtgcgccga
     4501 gttcgtcggg ccgcgcggtg gccggcgtgg acagcgccgt gtcgcccgcg gccaggcgcg
     4561 acatcacacg cgtgatgttc tcgatctcgc cgaccagccg ccgcacgtag cgcgtggcga
     4621 acgcgccgat cagcacgcac agcgccgtga gcacgagcat cagcgcctcg ctcgcgcgca
     4681 ccgccacgcg catgcgctcg ctgcggtcgg cagccgccag gcgcagctgc gcgacgtagc
     4741 gcgaggtctc gtcgctcagg tcgtcggcgt tggcgcgggt gatcgtgacg aggtagtcga
     4801 tctgcgcgtc gtagttcacc agcgcctggc ggcagcccag cacggagtcc ggcccttcgg
     4861 tcaacgcgac gagctcgcgc gcgtcctcgg gcgcgagcga cttcgtcgcc ccgtgctggc
     4921 gcgccgacag cagcagcgcc tcggcgtcag cctctaggcg gccgatcgtg gccgtgtccg
     4981 acaccgccgc ccccagcacc agcgtcgacc acaggctgga gatgcgcggg tcggccggct
     5041 cgccgtgcgc gggatgcagc acctggccga ggcggtccag gcgcttgagg ctggcgtcca
     5101 ggcgctcctg ttcgcgcagc ttgcgttgcg acagctcgat cagcgccggc aggtcctggt
     5161 gcacgctctg ctgcaggcga tcgatgaccg agcgcaggcg cggcgtggtc tcgccgatct
     5221 ccgacgtgct gcggcccacc tgcgccagca gggactcgac gacctggcgg tgcgtgtcga
     5281 gttccgcggc cgacagcgaa tcgcccagct tgggcgcggt gctcgcgagc tgggtggcgc
     5341 gctcggacag ctcgagcgcg tgcgagatcc gcggcagcag gtcgccctcg acgccggcca
     5401 gcgtggactg catgcgccgc aggccgaccc agcccaccgc ggtgagcagc acggtcgcac
     5461 ccagcagcag caggaaggcc gcgagcaggc gcgtgcgcac gcgccccagg cgccgcaccg
     5521 gccgttcgcc gccggccgcg gccgcaggtt ccacgacgcc ggccgcgggc atcagcatgg
     5581 cgatacgtcg accgcgaaca cgtagcccat cccgcgctcg gtgcagatga aggcgggatg
     5641 cttggggttg gactcgatct tgcggcgcag gcgcaggatc agcacgtcga tcgtgcggtc
     5701 gaacacctcg gtgtcgaggc tgcgcgtgtg ctccagcagc tggtcgcggg tccagatgcg
     5761 gcgcggctgg cgcacgaagg cctcgagcag gcggaactcg gcctgcgtga gggcgcacag
     5821 gctgccgtcg gcgcgatgca gctcgcgctc ggtcaggtcg agcgtccagt tgccgaagcg
     5881 caggcgctcg ttgttgcccg ccgcggccgg cgcgcgcgcg ggcactggcg cctgcggggc
     5941 cgggtcgtag cggcgcagca cggcgcgcac gcgggcgatg agctcgcgcg ggctgaacgg
     6001 cttgatgagg aagtcgtcgg ccgccagttc cagcagcagc acgcgatcgg tctcgtcgct
     6061 gctgccgctg atcatgatga tcgggatcgc ggacgtctgg cgcaactggc gcgccagcgt
     6121 caggccgtcc tcggccttga ggcgcgggtc cagcaccatc agcgcgtagt cgcggcccgc
     6181 catggcgcgc cgcgcgtcct caccgttgct cgcgccgtcg gcctcgaagc cggcgcgccg
     6241 cagcacgtcc tgcagcgagg ccaccatgtc cggctcgtcg tcgaccacca gcacgctggg
     6301 gcgcgccgtc tcgatcgcct ccaagactgt ctccaatttg aatgacgcca tgttctatcc
     6361 cgctcggcga ccgcccctcg tgcgggggcc tgccgagttg accacgcgtg tccggcaccc
     6421 gtgtttcaac tatttcaata tcgctcagag gcgggggcag cgcaacgcgc cgccgcgacc
     6481 gcgcccggtg ccatcgcggt ccggacctgg attaccctaa tgtgcatttc gcacacccga
     6541 acgaccatgg tctcgcagcc cggagccgga accaccgggc gcgccgccga ggcgcgcgat
     6601 cgagcacgcg ccgcggcggt ggtcaccagg actctcccgt cgttgttcat gcgcccgccc
     6661 ctcgcgggcg cctttcttgg acggcacgcg cggcgccgtg ctcgacaggc gatgcgctat
     6721 gctccgcccc gtgggatggg aatggggctt cccgacaacc gccgatcctg gctgatagct
     6781 cctgccgaca ccgggcgtcc gaacgcctgc ggcgggagcg tgcgttgtcc acgagcgcga
     6841 gcccgccgcg gcccaggatg tccacgacac tccaaccgcg aactcgaccc atgcacaaat
     6901 atctcctcgt cttcgtcggc ggcggcttcg gcgccgcgct gcgtcactt
//
LOCUS       DATCRO010000894         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_943710, whole genome shotgun sequence.
ACCESSION   DATCRO010000894 DATCRO010000000
VERSION     DATCRO010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943710"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VIN75_24300"
     CDS             complement(<1..1257)
                     /locus_tag="VIN75_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977357.1"
                     /translation="MIQADRLKVRQAAGNGEATVLEKLRDATNDASFDSLGAIRNGVA
                     FSHNGVTITPVGSGSDLAWKPTVTELATMGYLPAGWTATVSSLNAAPYTIAFTRVPAG
                     CAPSACNVEGLVVLQGPILAGASGSDGAVIGPILTRIGADSGVSLPTDASHIQGFGNT
                     WLADNPVSGQPAGVVAVRVGTTSSRFGQFIRIGDTRDPNLQGNLTVAGNTLFGDGTTR
                     SEFRSALQVDAQPVELRDGSGAACVSLRPDGTVDILCDGALSATTGVFKDASGHASTL
                     AGTGLVTNGSVTAQGGLSTPSMTAFGSDDPQALVVKTGDLFVRNPAGTALLRVATNGD
                     VTAGHDLVAGGMVQAQRLTLSGTVDEGDPCAPGQVAMMASGGLATCQGAGFRATSRYA
                     SLGAACATTGLQAVDAASGDALVCRGG"
     gene            complement(1373..1936)
                     /locus_tag="VIN75_24305"
     CDS             complement(1373..1936)
                     /locus_tag="VIN75_24305"
                     /inference="COORDINATES: protein motif:HMM:NF020384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HEY8977358.1"
                     /translation="MSQSQRRPVSALRPRHRQRGNALVFSMLGLVIGGIVLALGISYY
                     QNAQTNAQVQGTISEISAIIGGAQQNYGQYGYAGLTTAVAVGSRVIPDTRADAGGATA
                     TNSYSGAITLVDNSATTPSTAKLTYANVPASQCTQLVNGAQSLARRVTIGTTDVKPLD
                     GAIDVARVNTQCTSSANVAIDFVFGRT"
     gene            complement(2047..3177)
                     /locus_tag="VIN75_24310"
     CDS             complement(2047..3177)
                     /locus_tag="VIN75_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977359.1"
                     /translation="MNAARSLIAWMALADFRLTRAEFYRDFAEMYQRNEAMVSFLEGE
                     IANAGLTRQRSRARALRIVLGRHQDGDHASRVSHLLEGVVPRSDGLLLAAVDRAADKP
                     QALRALADAIDKQQRMQRLMLAYSALPALTLPICCALIALLGKVILVIDDATPVYAQE
                     ALWDGMNGWARDIAVFAQAWGPLTVLALAIALAAALWSLPRWRGAARLRVEGWPIYGL
                     YRDFQAGLLFSSMAMMLKTGETLQGSIDDVTQRASPWMRWHLGRVVDALDENPTGTID
                     AFRRGLLSAHLLSRAATLNRSSASFSDVLIQLGTTEGDRVLARVKRAAVIANFALVGL
                     FASVATFMGIASMTVPGTFASLMEPSNLMTLRAMYDATHSTH"
     gene            complement(3174..>3390)
                     /locus_tag="VIN75_24315"
     CDS             complement(3174..>3390)
                     /locus_tag="VIN75_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977360.1"
                     /translation="VWLRHVRNNDDDAALQHFRSTSNRQFDSPDMTGKTVFEHALYKA
                     MQGEIDVRNCEEFDGFERFEILSRDRP"
BASE COUNT          513 a         1157 c         1207 g          513 t
ORIGIN      
        1 cccgccgcga cagaccagcg catcgccgga ggccgcatcg acggcctgca ggccggtggt
       61 ggcgcaggcg gcgcccaggc tcgcatagcg cgaggtcgct cgaaagcccg cgccctggca
      121 ggtggcgagg ccaccgctgg ccatcatcgc gacctggccc ggcgcgcagg gatcgccctc
      181 gtcgaccgtg cccgacaagg tcagccgctg cgcctgcacc atcccgccgg cgaccaggtc
      241 gtggcctgcg gtgacgtcgc cgttggtggc gacgcgcagc agcgccgtgc cggccggatt
      301 gcgaacgaac aggtcgcccg tcttgacgac cagcgcctgc ggatcgtcgc tgccgaacgc
      361 ggtcatcgac ggcgtcgaca gtccgccttg cgcggtcacg ctcccgttgg tcaccagccc
      421 cgtgcccgcg agcgtcgacg catgacccga cgcgtccttg aacacacccg tggtcgccga
      481 cagcgccccg tcgcacagga tgtcgaccgt gccatcggga cgcagcgaca cgcaggccgc
      541 accactgccg tcgcgcagtt cgaccggttg ggcatcgacc tgcagcgcgg agcggaactc
      601 gctgcgcgtc gtgccgtcgc cgaacagcgt attgccggcc acggtgaggt tgccctggag
      661 gttcggatcg cgcgtgtcgc cgatgcggat gaactggccg aatctggagg acgtcgtgcc
      721 gacccggaca gcgacgacgc cggccggttg gcccgacacc gggttgtccg caagccaggt
      781 gttgccgaag ccctggatgt ggctggcgtc cgtcggcagc gagacgccgg aatcggcgcc
      841 gatgcgcgtc aggatcgggc cgatcaccgc accgtcgctg ccgcttgctc ctgcgaggat
      901 cgggccctgc aacacgacca ggccctcgac gttgcaagcg gagggcgcac agcccgcggg
      961 cacgcgcgtg aaggcgatcg tgtacggcgc ggcgttcagg ctcgacaccg tcgccgtcca
     1021 gccggccggc aggtagccca tcgtcgccag ctccgtgacc gtcggcttcc aggccaggtc
     1081 actgccgctg ccgaccgggg tgatcgtgac gccgttgtga ctgaacgcga cgccgttgcg
     1141 gatggcgccc aggctgtcga agctggcatc gttggtggcg tcgcgcaact tctcgaggac
     1201 ggtcgcctcg ccgttgcctg ccgcctggcg gaccttgagc cgatcggcct ggatcatgcc
     1261 cgccgcaccg agcgccgaga cgagcaggcc gagcagcgcg aatacgagcg cattgccacg
     1321 ctgccgggaa cggcaggcac gggaaccggg tgggcggcgc ggcgacatgg gctcaggtgc
     1381 ggccgaacac gaagtcgatc gcgacgttgg cagacgaggt gcactgcgtg ttgacacgcg
     1441 caacatcgat ggcgccgtcc agcggcttga cgtcggtcgt gccgatcgtg acacgccgcg
     1501 ccaggctctg cgcgccgttg accagctgcg tgcattgcga ggccggcacg ttggcgtagg
     1561 tgagcttggc agtgctgggc gtggtggccg agttgtcgac cagcgtgatg gcgcccgaat
     1621 agctgttcgt ggccgtggcg ccgccggcgt cggcacgcgt gtcggggatc acgcgcgagc
     1681 cgacggccac ggccgtggtg agccccgcgt agccgtattg cccgtaattc tgttgtgcac
     1741 caccgatgat ggcgctgatc tcgctgatcg tgccctgaac ctgcgcgttg gtctgcgcgt
     1801 tctggtagta gctgatgccc agcgcgagca cgatgccgcc gatgaccagg cccagcatgc
     1861 tgaacaccag ggcgttgccg cgctggcgat gacgcggacg aagcgcggac acggggcggc
     1921 gttgggactg ggacatcttc ggttctcctt ttgggctggc gtggacggat cgtcgtccgg
     1981 aagatgcacg cggcgcagat ggccggcgtg cgcgggaagc tcgggcggcc gaggacggcg
     2041 cgccggtcaa tgcgtcgaat gcgtggcgtc gtacatcgcg cgtagcgtca tcaggttgga
     2101 cggctccatc aacgaggcga acgtcccggg caccgtcatc gacgcgatgc ccatgaaggt
     2161 tgccacggag gcgaaaagtc cgaccagcgc gaaattcgcg attacggccg ctcttttcac
     2221 gcgggcgagc acgcgatcgc cttccgtcgt tccgagctgg atcagcacgt ccgagaacga
     2281 ggcgctggag cggttcagcg tggccgcgcg cgaaagcagg tgcgccgaca gcagcccgcg
     2341 ccggaacgcg tcgatcgtgc cggtcgggtt ctcgtcgagc gcatccacca cccggccgag
     2401 gtgccagcgc atccagggcg aggcgcgctg cgtgacgtcg tcgatgctgc cttgcagcgt
     2461 ctcgccggtc ttgagcatca tcgccatcga actgaacagg aggccggcct ggaagtcgcg
     2521 gtacaggccg tagatcggcc agccttcgac gcgcagccgg gccgcccctc gccagcgtgg
     2581 cagcgaccag agagccgcgg cgagcgcgat ggccagcgcc agcaccgtca acgggcccca
     2641 cgcctgggca aagaccgcga tgtcgcgggc ccagccgttc atgccgtccc acaaggcctc
     2701 ctgcgcgtag accggcgtcg cgtcgtcgat gaccaggatc accttgccca gcaaggcgat
     2761 gagcgcgcag cagatcggca gcgtgagggc cggcaacgcc gagtacgcca gcatgagtcg
     2821 ctgcatgcgc tgctgcttgt cgatcgcatc ggcgagcgcg cgcaaggcct gcggcttgtc
     2881 ggctgcgcgg tcgaccgcgg ccaacagcag cccgtccgag cgcggcacga cgccctcgag
     2941 caggtgcgat acccggctcg catggtcgcc gtcctggtgc cggcccagca cgatgcgcag
     3001 cgcgcgcgcc cggctgcgct ggcgcgtgag gccggcgttg gcgatctcgc cttccaggaa
     3061 gctcaccatc gcctcgttgc gctggtacat ctcggcgaag tcgcggtaga actccgcccg
     3121 cgtcagccgg aagtctgcca gcgccatcca ggcgatcagg ctccgggcgg cgttcatggg
     3181 cgatccctcg acaggatctc gaagcgctcg aagccatcga actcctcgca gttgcgcacg
     3241 tcgatctcgc cctgcatcgc cttgtagagc gcgtgctcga agaccgtctt gccggtcatg
     3301 tccgggctgt cgaactggcg gttgctggtc gatcggaagt gctgcagcgc ggcgtcgtcg
     3361 tcgttgttgc gcacgtggcg cagccagacg
//
LOCUS       DATCRO010000895         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_527623, whole genome shotgun sequence.
ACCESSION   DATCRO010000895 DATCRO010000000
VERSION     DATCRO010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527623"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="rplS"
                     /locus_tag="VIN75_24320"
     CDS             <1..294
                     /gene="rplS"
                     /locus_tag="VIN75_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8977361.1"
                     /translation="AFAPGDTVIVNVNVVEGTRKRVQAYEGVVIARRNRGLNSAFIVR
                     KISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYLRDRSGKSARIKEKLA"
     gene            381..1508
                     /locus_tag="VIN75_24325"
     CDS             381..1508
                     /locus_tag="VIN75_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY8977362.1"
                     /translation="MSPSSLPEPYRVLVIGGYGFFGRRLVEWLSLQRGLHVIVCGRSA
                     AQAEALVADLRPGARCSLEGVALDALSDAFPGALARLRVKTVVHTSGPFQGQDHRVAQ
                     ACIAAGVHYVDLADGREFVAGIGTLDDAARAAGVCVLSGASTVPALSAAAADALVAGW
                     QRVDAIDMGISPGNRTERGLSTIRAVLSYCGRPIPHADGIARTGWLRLRLHRYPARIG
                     WRPLSPCDVPDLALLPERYAGRPVVRFGAGLELLVLHLGANAMALVGRLGLVRDWSVH
                     ASRLKRWSDRLAAFGTDAGAMHVTARGLDAQGRPVHRTWTLVATDGDGPWVPTLAAAA
                     LVRKLAAADVPASGARPCIGELSLADFARETSGRAIAWAIA"
     gene            1505..2086
                     /locus_tag="VIN75_24330"
     CDS             1505..2086
                     /locus_tag="VIN75_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEY8977363.1"
                     /translation="MSGPSLFERVLGSHFAALSPALQCFHRLCGLHELHGLVDTDAPA
                     TLAGRLLARCLGTPPRATHGAIRFVLEAAPDVESWTRHFPGRTLRSRLHFTSGRVVER
                     MGPARLAFALDVDAGRLRLHLVDMRFLGIRCPAWLRPRIVAEESGGQGADGRERLHFR
                     VEARVPGVGQVVGYRGHLELPGGDASEAASPRG"
     gene            2157..2924
                     /locus_tag="VIN75_24335"
     CDS             2157..2924
                     /locus_tag="VIN75_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8977364.1"
                     /translation="MSTPEPPSKPSTPTRPLIREPRLAPVIGSDTHLPAVAPERLTPE
                     AVRARFAPTLGAIARTPADGADAAPRWTPEFTGDGQRLAGRALRPAAVLVPLVRRPAG
                     LAVLLTRRTDHLNDHAGQVSFPGGRTDPEDADAVATALREAHEEVGIAADEIEVIGQL
                     PAYTTVTAYEVTPVVGLLDPPRALRLDPFEVAEVFEVPLAFLMDPAHHRRHAVEVDGL
                     ARQFISMPWGRDLRGEPYFVWGATAAMLRNLYRFLAH"
     gene            2989..>3918
                     /locus_tag="VIN75_24340"
     CDS             2989..>3918
                     /locus_tag="VIN75_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEY8977365.1"
                     /translation="MSFIAVLFALLLEQIKPLPRNNVVHYSLASWVGWTGRNFDAGKP
                     QHAKVVWIVTVIAPALAAALVYIGIAHYNTIAALVFDIAILYLTLGFRQFSHYFTDIR
                     TALEGGDEPEARRLLAEWRHLDASELPRTELLRHMIEHSLLAAHRHVFGVFFCFIVFS
                     ALGLGPAGAVLYRMAEFASRYWAFRSKSLDTPINENLMALSQRLFSLIDHIPARLTAF
                     GFAVVGNFQDAIDGWKRDAGLWKHANEGIILAAAAGAVGVQLGGATPPGVTPDRSKTF
                     TSGEASLASESAGSTTQGMPPQLGHLGIVVGLVW"
BASE COUNT          483 a         1459 c         1400 g          576 t
ORIGIN      
        1 gctttcgccc ccggtgacac ggtcatcgtc aacgtcaacg tcgtcgaagg cacgcgcaag
       61 cgcgtccagg cgtacgaagg cgtcgtgatc gcccgtcgca accgcggcct gaactcggct
      121 ttcatcgttc gcaagatctc cagcggcgaa ggcgtggaac gcacgttcca gctgtacagc
      181 ccgctgatcg cctcgatcga ggtcaagcgc cgcggcgacg tccgccgcgc caagctgtac
      241 tacctgcgcg atcgctcggg caagtcggcg cgcatcaagg aaaagctggc ctgaggccgc
      301 ggcgtccctc gcggacgcca tgtcccgcca cgacaagccc ggccctgccg ggctttttca
      361 tttccggatc ccgtcacccg atgagcccgt cgtccctgcc cgaaccgtat cgcgtcctgg
      421 tcatcggcgg ctacggcttc ttcggtcggc ggctggtcga gtggctgtcg ctgcagcgcg
      481 gtctgcacgt catcgtctgc ggacgctcgg cggcgcaggc cgaggcgctg gtcgccgact
      541 tgcggccggg cgcgcgctgc agcctggagg gcgtggcgct ggacgcgttg tccgatgcct
      601 ttccgggtgc gctcgcacgg ctgcgggtga agacggtcgt gcacacgagc ggcccgttcc
      661 agggccagga tcatcgcgtg gcgcaggcct gcatcgccgc gggcgtccac tacgtcgatc
      721 tcgcggacgg ccgtgaattc gtggccggca tcggcacgct ggacgacgcg gcacgggcgg
      781 cgggcgtctg cgtcctgagc ggtgcgagca ccgtgccggc gctgtccgcg gcggccgccg
      841 acgcgctggt cgcgggctgg cagcgcgtcg acgcgatcga catgggcatc agccccggca
      901 accgcaccga gcgcgggctg tcgacgatcc gggcggtgct gagctattgc ggccggccga
      961 tcccgcatgc ggacggcatc gcgcgcacgg gctggctccg gctgcgactg caccgctatc
     1021 ccgcgcggat cggctggcgc ccgttgtcgc cgtgcgacgt gcccgacctc gcgctgctgc
     1081 ccgagcgcta cgccggccgg cccgtcgtgc gcttcggggc cgggctggaa ctgctcgtgt
     1141 tgcacctggg cgccaacgcg atggccctcg tcggccggct cgggctcgtg cgcgactggt
     1201 cggtgcacgc gtcccggctc aagcgctggt ccgatcgcct ggccgcgttc ggcacggacg
     1261 ccggcgcgat gcacgtgacg gcgcgcggcc tggacgcgca gggccggccc gtgcaccgca
     1321 cgtggacgct ggtcgcgacc gacggcgatg gcccctgggt gccgacgctc gccgcggccg
     1381 cgctggtgcg caagctcgcg gcggccgacg tgccggccag cggcgcgcga ccctgcatcg
     1441 gcgagctgtc gctggcggac ttcgcccggg agacgagcgg ccgcgccatc gcctgggcca
     1501 tcgcatgagc gggccgtcgc tgttcgagcg cgtcctgggc agccacttcg ccgcgctgtc
     1561 gcctgcgttg cagtgcttcc accgcctgtg cggactccac gagctgcacg gcctggtcga
     1621 caccgacgcc ccggccacgc ttgccggccg gctgctggcg cgctgcctgg gcacgccccc
     1681 gagggccacg catggcgcga tccgcttcgt gctggaagcc gcccccgacg tcgagagctg
     1741 gacgcgccac ttccccggcc ggacgctgcg ttcgcgcctg cacttcacgt ccggccgcgt
     1801 ggtcgagcgc atgggcccgg cgcgcctggc gttcgcgctg gacgtcgacg ccggccgcct
     1861 gcgcctgcac ctggtggaca tgcgcttcct gggcatccgc tgtccggcct ggctgcggcc
     1921 ccgcatcgtc gccgaggagt cgggcggaca gggcgccgac ggccgcgagc ggctgcactt
     1981 ccgcgtcgag gcgcgcgtgc cgggcgtggg acaggtcgtg gggtatcgcg gccacctcga
     2041 gctgcccggc ggcgacgcga gcgaagctgc ttcgccacgg gggtgaaacg cggcccggcg
     2101 tgcacggcgg gcgcgcaggc cggacaatcg tcacgacctt gcagaacaat gaccgcatgt
     2161 ccacgcccga gccgccgtcc aagccgtcca cgcccacgcg gccgctgatc cgcgagccgc
     2221 gcctcgcgcc ggtcatcggc agcgacacgc acctgcccgc cgtcgcgcct gagcgcctga
     2281 cgcccgaggc cgtgcgcgcg cgcttcgcgc cgacgctggg cgcgatcgcc cgcacgcccg
     2341 ccgacggcgc cgacgccgcg ccccgctgga cgcccgagtt caccggcgac ggccagcgcc
     2401 tggcgggccg cgcgctgcgg cccgcggccg tgctggtgcc gctggtgcga cgacccgcag
     2461 gactggccgt cctgctcacg cgccgcaccg accacctcaa cgaccacgcc ggccaggtga
     2521 gctttcccgg cggacgcacc gatccggagg acgccgacgc cgtcgcgacc gcgctgcgcg
     2581 aggcgcacga ggaggtgggc atcgcggccg acgagatcga ggtgatcggc cagctgcccg
     2641 cctacaccac cgtcaccgcc tacgaggtca cgccggtggt ggggctgctc gatccgccgc
     2701 gcgcgctcag gctcgatccg ttcgaggtgg ccgaggtgtt cgaggtgccg ctcgccttcc
     2761 tgatggaccc ggcacaccac cggcgccacg ccgtggaagt ggacgggctg gcgcgccagt
     2821 tcatctcgat gccgtggggc cgcgacctgc gcggcgagcc gtacttcgtc tggggcgcga
     2881 ccgcggcgat gctgcgcaat ctctaccgct tcctggccca ttgaagcggc ggccgcgagg
     2941 ccgccgggca actcggccgt ggccatgctg cggctatgat tcgaggcaat gagtttcatc
     3001 gccgtcctct ttgccttgct gctcgagcag atcaagccgc tgccgcgcaa caacgtcgtg
     3061 cactactcgc tcgcctcgtg ggtgggctgg acgggccgca acttcgacgc cggcaagccg
     3121 cagcacgcca aggtcgtgtg gatcgtcacc gtgatcgcgc ccgcgctggc ggccgcgctc
     3181 gtctacatcg gcatcgcgca ctacaacacg atcgcggcgc tcgtgttcga catcgcgatc
     3241 ctgtacctca cgctgggctt ccgccagttc agccactact tcaccgacat ccgcacggcg
     3301 ctggaaggcg gcgacgagcc cgaggcgcgc cggctgctgg ccgagtggcg ccacctggac
     3361 gcctcggagc tgccgcgcac cgaactgctg cgccacatga tcgagcactc gctgctggcc
     3421 gcgcaccgcc acgtgttcgg cgtgttcttc tgcttcatcg tgttctcggc gctgggcctg
     3481 ggcccggcgg gcgcggtgct gtaccgcatg gccgaattcg cgtcgcgcta ctgggcgttc
     3541 cgcagcaagt cgctcgacac gccgatcaac gagaacctga tggcgctgtc gcagcgcctg
     3601 ttcagcctga tcgaccacat cccggcgcgc ctgaccgcgt tcggcttcgc cgtcgtcggc
     3661 aacttccagg acgccatcga cggctggaag cgcgacgccg ggctctggaa gcatgccaac
     3721 gagggcatca tcctggccgc agcggcgggc gccgtcggcg tgcagctggg tggggcgacg
     3781 ccgccgggcg tgacgccgga ccgttccaag accttcacca gcggcgaggc gtcgctggcg
     3841 tcggagtcgg ccggcagcac cacgcagggc atgccgccgc agctggggca cctgggcatc
     3901 gtcgtcggcc tcgtgtgg
//
LOCUS       DATCRO010000896        13534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_617814, whole genome shotgun sequence.
ACCESSION   DATCRO010000896 DATCRO010000000
VERSION     DATCRO010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617814"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VIN75_24345"
     CDS             <1..992
                     /locus_tag="VIN75_24345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8977366.1"
                     /translation="GIRSARNVLNQGLELSVEDDGAGCEPARLDDPAPARSGIGLTAL
                     REIALHVVGALLFGAAWQACDYGVSALLYGRVFANVNLEQTVLWRAIWGVIAYAAIAT
                     AFTAWLQARKARAALLAAAHAESALARAELAVISGKLNPHFLFNTLNSLIALTRKDAQ
                     AAEAALMRFSSMLRYVLDTKRSAADRVALGEEIEFVRDYLALESLRLGKRLRVEWELD
                     PETFADEIPPLSLQPLVENAIQHGIAPRVDGGRVGIRSARNVLNQGLELSVEDDGAGC
                     EPARLDDPAPARSGIGLTALRRRFALDYEGRARLQIRTQPGAGFRVDLWIPQP"
     gene            989..1732
                     /locus_tag="VIN75_24350"
     CDS             989..1732
                     /locus_tag="VIN75_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8977367.1"
                     /translation="MTELTRVLIAEDEPLAAEALADWVARMPTLQLVASCGDGASALA
                     QIRALQPDLVLMDIQMPVLTGLQVMQALAGDAHQPAVIFTTAYDEHALTAFELHAVDY
                     LLKPFTHERFVEAIEHVQRDRGAVASAVDALQAPDNAPLTRVLVRDQGRIFPINVDAI
                     EHLRSDTKYTALAVKGKSYLVRLPIVTFEQKLDPARFVKLHRSCIVNLDFVESLTPDE
                     NSQLVARMTDGTLITANRDVSRKLREQSI"
     gene            1729..2784
                     /locus_tag="VIN75_24355"
     CDS             1729..2784
                     /locus_tag="VIN75_24355"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEY8977368.1"
                     /translation="MNRHLSIITCVALSLSGGLALAQEASSVEDAQRQVEQAQQQAEQ
                     AQQRAEQARRDAEQARRDAEQARKAADQARRQALKQAEQARRDAQKQVEQAQKQAQDD
                     ARAQARAAADDARAQADEARARGRMQADSRGQGVSSGEGKVYAPGPFDSLTVDGAGQV
                     RIVQADRDEVFVPGDESAQDSVDVELKGSRMHIDLPGGWKFWKGGNGALVEVRMRHLN
                     KLTLSGSNDVVAPGPITGDQLTVSMAGSGLARFDQLQVGSLNFEISGAGEGQLMGKVD
                     QLRLNVSGKGKIGAEQLRTGSADVSISGVGNASLWALSDLRVQISGAGHVDYWGQPKV
                     QKSISGFGSVDARGDKK"
     gene            3150..4076
                     /locus_tag="VIN75_24360"
     CDS             3150..4076
                     /locus_tag="VIN75_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEY8977369.1"
                     /translation="MVITLFGPEAAPWVEKLPLKHAVRVPGLSADYPAVRCTDSGVCV
                     MTTGMGHANAAASAMALALSRQFDLSHTYFLVTGIAGIDPNQGTLGSAAWARYLVDFG
                     LQQEFDAREAPPAWAGGYVGIGAADPETKPKLEYGTEVFQLDEALLQRALALSRGAPL
                     KDNDTAAAYRRQYPQAAAQAAPGVLQCDTLAGDTWWHGDRLGERATRWTRLLTDGKGT
                     YCTTQQEDNATYEALRRGAAAGRLDLKRVAVLRTGANFDRPHPGQTDQESLNARSGGF
                     PIATANLYEAAWPLVSDIVARWPQWREGVPAR"
     gene            4402..6663
                     /locus_tag="VIN75_24365"
     CDS             4402..6663
                     /locus_tag="VIN75_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8977370.1"
                     /translation="MTTRTTIRPAMRPTMPLKPLSLVLLLAFARAAHAQAAAPADAAS
                     AAEAGKLQTITVTAQRITEDVRQVPISVSTLGADALDVFGSSGDDIRALSGRVPSLNI
                     ESSFGRAFPRFYIRGYGNTDFHLNASQPVSLVYDDVVQENPILKGFPIFDTEQVEVLA
                     GPQGTLFGRNTPAGVVKVDSVAPSRVAGGYASATFGNFHTINLEGAYGMPLGTDWAIR
                     ASTQLQHRGDWVANTAPTEQTGRLEGYDDDALRVQALYKPTATFSALFNVHGRDLHGT
                     ARLFRANIIKPGTNDLVDGFDPYAVSFDGQNRQSLNTSGASAKLRWALGDYVLNSITG
                     WEHVQAFSRGDVDGGYGASYAPPFGPGFIPFSDETSDALKGHSQVSQEFRVEHADSSP
                     LSWQAGLYLFREKYAIDSLSYDTLFGAPPTEVDATQVNNAWAVFGSAGYQLTADFKLR
                     AGVRWTQDRKSLVTIPISDTKVNAAKGLAAGTSDSKTSWDLSGTYALSKDVNVYARVA
                     TGFRGSSIFPASDFNVLTAAKPETTTSYEFGTKADLFDRRARISADVFHYEVKNQQLS
                     AVGGDGNGTELLNAKKVIGQGLELNLDAYLTPSLLVTFNGSYNFTRIKDPSLSVAPCG
                     SCTVTDPLDANGHALIDGNPLPQAPKYVLNLNARYGIPVGNGEYFVYTDWSYRSEVNF
                     FLYESKEFTGKAMTIGGLRAGYDWSDGKYEVAGFVRNLTNQIRVTGGIDFNNLTGFIN
                     DPRTYGVQFKASF"
     gene            6735..7775
                     /locus_tag="VIN75_24370"
     CDS             6735..7775
                     /locus_tag="VIN75_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY8977371.1"
                     /translation="MKKLFVAAVLAAGLSSAFAARPAAKAACLTECTPRIGIVSAFGQ
                     EAAVLVAQTRSPHTWIINGNRFTTGMLRGEPVVIVLSGVSMVNSAMVTQLMVDHFKVG
                     RLVMSGIAGGVDPAHHVGDVMIPDRWAMPLEVFWNRDNVLPATCGKEADVSCLGLKLA
                     SVDGKPVPPFYLATPAAPMPSGLFMRENFVMTAANAPAGEFRFDYPVDAAMLAVAREI
                     RPALARCGPKAVKSPGAEPDPALCVKTTPQVIVGGRGVSGSAFLANPQYRRYLFEHLQ
                     AQVFEMETAALAHVAYANGIPYIAFRSLSDLAGAEEFDPDAVALFSSGLAETNEAAVT
                     LAFLDGWHHRRR"
     gene            7988..9034
                     /locus_tag="VIN75_24375"
     CDS             7988..9034
                     /locus_tag="VIN75_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977372.1"
                     /translation="MTFERILTAAAAAFVLAGAATTASAESSGQHTLVLTTSNATTNE
                     LVVLDTAGNVLRRLPTQGQGGVAGNAGGIAASRDRVAVVNFGSSNVSVFTRDDGGRLF
                     RLERVIPTVGSPVSVAFGHDHLYVLTTTGVESHRAGSWGVSPAADGRSALLHADGSAA
                     QVGVLNDELVFSEKSNAIETARLAGDGAVVGAAALVAAIPANVDAPFGLAARGDDAYV
                     TIAHADEISLVRHDTVLATTPSMTQHAPCWVALDGPFLFSANSPSKSVSRYAVYGQKV
                     VQDAPVVAQFAGSPTDIAYRAGFAAVVDAGAGLSRVSIFKVDEDGNFTLRGVADLGAA
                     HTNGVAIVDGDAWR"
     gene            9175..9624
                     /gene="msrB"
                     /locus_tag="VIN75_24380"
     CDS             9175..9624
                     /gene="msrB"
                     /locus_tag="VIN75_24380"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8977373.1"
                     /translation="MPEYRKTPEALARLTPIQRHVTQDSGTERPGTGELLHNKEAGIY
                     VDVVSGEPLFASSDKFESGCGWPSFTKPITQGFVDEFRDTTHGMIRTEVRSHHGDSHL
                     GHVFPDGPRDRGGLRYCINSASLRFVPREQMEAQGYGEYLPQVEDVQ"
     gene            9731..10234
                     /gene="msrA"
                     /locus_tag="VIN75_24385"
     CDS             9731..10234
                     /gene="msrA"
                     /locus_tag="VIN75_24385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8977374.1"
                     /translation="MTTERAVLAGGCFWGMQELIRHKPGVIETRVGYTGGDVEDATYR
                     RHGTHAEGIEIIFDPAVISYRDLLEFFFQIHDPSTKNRQGNDVGMSYRSAIYYTTPEQ
                     KAVAEETIRDVDASGLWPGKVVTELEPAGAFWEAEPEHQDYLQMFPNGYTCHFPRPGW
                     KLPRRNG"
     gene            complement(10262..11443)
                     /locus_tag="VIN75_24390"
     CDS             complement(10262..11443)
                     /locus_tag="VIN75_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8977375.1"
                     /translation="MSSSERERLARWRLVIGSEAEAACGALAGQAIEMDQALTALYDV
                     QGSAGGLGGGTRGGRGASQPSVARWLGDIRKYFPSSVVQVMQHDALQRLNLREMLLQP
                     EMLRSAQPDVHLVASLIALSRVIPAGTRDTARMVVRQVVDDLMKRLEEPMRSAIAGAL
                     DRSQRNRRPRHAQIDWNRTIRANLKHWQPEYRTIVPETLHGYGRKARRPQREIILCVD
                     QSGSMANSVVYSSIFGAVMASLPAVSTKLVVFDTAVVDMSDQLDDPVELLFGVQLGGG
                     TDIDGAVGYCQSLVREPRNTIFVLISDLYEGGVEANLLRRASALVDSGVQCITLLALS
                     DEGRPAYDHALAAKLAALGMPSFACTPDAFPALMAAAIRRDDVAQWAAANGLVNSPSA
                     R"
     gene            complement(11440..>13534)
                     /locus_tag="VIN75_24395"
     CDS             complement(11440..>13534)
                     /locus_tag="VIN75_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEY8977376.1"
                     /translation="ARFIDLPLAHELAQAKAEREAASKAEGGTVPSEAPDAEAAPPPI
                     AAPAPDPGQDAAEPLGRRDPLSWLAQAAGYGDGESWWNHMVEERADGAELFEAIGTAM
                     ASLRAELGEDHLSPELARRDALREAHMRQCVRAARKQGFARIAVVCGAWHVPALGATV
                     AAKADAALLKGLPRLKVQSTWVPWTYRHLTRASGYGAGIASPGWYEHLWRTHDRPARR
                     AVGWLARVARLMRERELDCSSAHLIEATRLADTLAALRDRPAPGLPELDEASRTVLTL
                     GDDAALQFIRDALVVGDHLGSVPPAVPTVPLQRDVDALQKRLRMKPEALRKTLDLDLR
                     SDNDLARSHLLHRLALLDVPWGTLAKVGRSARGTFHEVWTLQWQPEFAIRLIEASLWG
                     QTVEQAATARTQARCAEAGTLAALSTLVDQALLADLDRAVAAATQALEDRAAITGDAA
                     QLLATLPPLANVFRYGNVRQTDAALVAHVLDGLAVRAAIGLPVACVGLDDGAAESLRD
                     VVLSAQAAIALRDGAETTQAWRRALAHVADLAGAHDLLKGVAARLLLDAGEWPADKAA
                     TAMSLNLSAGAEPAHAAAWLEGFLNRNAVVLLHDAAVWSLVDDWLAGLSDDHFIRVLP
                     LVRRTFSAFAATELRDLSQKARHKPGAAHAAAGPAGAHGELFAAWDLERAALPLPMLR
                     TLLGLPEPHTNGGPP"
BASE COUNT         2052 a         4830 c         4779 g         1873 t
ORIGIN      
        1 tgggcatccg cagcgcgcgc aatgtcctga accagggcct ggaactgagc gtcgaggacg
       61 acggcgccgg ctgcgagccc gccaggctgg acgacccggc gcccgcgcgc tcgggcatcg
      121 gcctgaccgc gctgcgcgag atcgcgttgc acgtggtggg cgcgctgctg ttcggcgcgg
      181 cctggcaggc gtgcgactac ggcgtctcgg cgctgctgta cggccgtgtc ttcgccaacg
      241 tgaacctcga gcagacggtg ctgtggcgtg cgatctgggg cgtgatcgcg tacgccgcga
      301 tcgccaccgc gttcacggcc tggctgcagg cgcgcaaggc gcgcgcggcc ctgctcgccg
      361 cggcgcatgc cgaatcggcc ctggcgcgcg ccgagctggc cgtcatcagc ggcaagctca
      421 atccgcactt cctgttcaac accctcaact cgctgatcgc gctcacgcgc aaggacgccc
      481 aggccgcgga ggccgcgttg atgcgctttt ccagcatgct gcgctacgtg ctggacacca
      541 agcgcagcgc cgccgaccgc gtggcgctgg gcgaggagat cgagttcgtg cgcgactacc
      601 tggcgctcga gagcctgcgc ctgggcaagc gcctgcgcgt ggaatgggag ctcgaccccg
      661 agaccttcgc cgacgagatc ccgccgctga gcctgcagcc gctggtggag aacgccatcc
      721 agcacggcat cgcgccgcgc gtggacggcg gccgcgtggg catccgcagc gcgcgcaatg
      781 tcctgaacca gggcctggaa ctgagcgtcg aggacgacgg cgccggctgc gagcccgcca
      841 ggctggacga cccggcgccc gcgcgctcgg gcatcggcct gaccgcgctg cgccgccgct
      901 tcgcgctcga ctacgaaggc cgggcccgcc tgcagatccg cacccaaccc ggcgcgggct
      961 tccgcgtcga cctctggata ccccaaccat gaccgaactc acccgagtcc tgatcgccga
     1021 ggacgaaccg ctcgccgccg aggcgctggc cgactgggtc gcccgcatgc cgacgctgca
     1081 gctcgtggcc agctgcggcg acggcgccag cgcgctcgcg cagatccgcg ccctgcagcc
     1141 cgacctcgtg ctgatggaca tccagatgcc cgtgctgacc ggcctgcagg tgatgcaggc
     1201 gctggccggc gacgcgcacc agccggcggt catcttcacc accgcctatg acgagcacgc
     1261 gctgaccgcc ttcgagctgc acgcggtcga ctacctgctc aagccgttca cgcacgagcg
     1321 cttcgtcgag gcgatcgagc acgtgcagcg cgaccgcggt gccgtcgcca gcgccgtcga
     1381 cgcgctgcag gcgcccgaca acgcgccgct cacgcgcgtg ctggtgcgcg accagggccg
     1441 catcttcccg atcaacgtgg acgccatcga gcacctgcgc tcggacacca agtacaccgc
     1501 gctggccgtc aagggcaaga gctacctcgt gcgcctgccc atcgtcacct tcgagcagaa
     1561 gctcgatccg gcccgtttcg tcaagctgca tcgcagctgc atcgtcaacc tggacttcgt
     1621 cgagtcgctg acgcccgacg agaactcgca gctcgtcgcg cgcatgaccg acggcacgct
     1681 catcaccgcc aaccgcgacg tttccaggaa gctcagggag caatcgatat gaaccgccat
     1741 ctttccatca tcacctgcgt cgcgttgtcg ctgtcgggcg gcctggcgct cgcccaggag
     1801 gcgtcgtcgg tcgaggacgc gcagaggcag gtggagcagg cccagcagca ggccgagcag
     1861 gcgcagcaac gcgcggagca ggcgcgccgg gacgcggaac aggcccgtcg cgatgcggag
     1921 caggcgcgaa aggccgccga ccaggccagg cgccaggcgc tgaagcaggc cgagcaggcc
     1981 aggcgcgatg cgcagaagca ggtcgagcag gcgcagaagc aggcccagga cgacgcgcgg
     2041 gcccaggcgc gcgcggcggc cgacgacgcc cgggcgcagg ccgacgaggc acgggcgcgc
     2101 ggccgcatgc aggccgacag ccgagggcag ggcgtgtcgt ccggggaggg caaggtctac
     2161 gcgccggggc cgttcgacag cctcaccgtg gacggcgccg gccaggtgcg catcgtccag
     2221 gccgatcgcg acgaggtctt cgtgccgggc gacgagagcg cccaggattc ggtggacgtc
     2281 gaactcaagg ggagccggat gcacatcgac ctgcccggcg gctggaagtt ctggaagggc
     2341 ggcaacgggg cgctggtgga ggtacgcatg cgccacctga acaagctgac gctgtccggc
     2401 tcgaacgacg tcgtcgcccc cgggccgatc accggcgacc agctgaccgt cagcatggcc
     2461 ggctcgggcc tggcccggtt cgaccagctg caggtgggca gcctgaactt cgagatctcc
     2521 ggagcgggcg agggccagct gatgggcaag gtcgaccagc tccggctgaa cgtgtccggc
     2581 aagggcaaga tcggcgcgga gcagttgcgc acgggcagcg cggatgtctc gatcagcggc
     2641 gtcggcaacg cgtcgctgtg ggcactgagc gacctgcggg tgcagatctc cggagccggg
     2701 catgtcgact actgggggca gcccaaggtg cagaagtcga tctcggggtt cggatcggtc
     2761 gatgctcgtg gcgacaagaa atgatccatc aaaacaatga cttgcgtcgt gaacatgaat
     2821 tgatgatgcg aacacgggat ccttgacatc aatacattac atgagattgt taaagtgatt
     2881 tcttggacgg tggatcaatg agatgggatt gatttctcaa gcatgacttg agaaacatga
     2941 tgatcgccgc cgaccggccc gggacttgcg tccgccccgc acgccgtcgc gcacaatgcg
     3001 cggcaccgct tcaccgggga tccgtcttga atctgctttc ttcgctgtct cgccgcgcga
     3061 tcgccggcct cctctcgctg ctggccgccg tcgcgccgtt cgcggccgcc gcatccggca
     3121 aggccgccgc gccgcggccc gtgaaggtga tggtcatcac gctgttcggg cccgaggccg
     3181 cgccatgggt ggagaaactg cccctcaagc acgcggtgcg ggtgccgggg ctgtcggcgg
     3241 actatcccgc ggtcaggtgc acggacagcg gcgtgtgcgt catgacgacc ggcatggggc
     3301 acgccaacgc cgcggcctcg gccatggcgc tggcgctgag ccggcagttc gacctgtcgc
     3361 acacgtactt cctggtgacg ggcatcgccg gcatcgaccc gaaccagggc acgctgggca
     3421 gcgcggcctg ggcgcgctac ctggtcgact tcggcctgca gcaggaattc gacgcccgcg
     3481 aggccccgcc cgcgtgggcc ggtggctacg tcggcatcgg cgccgccgac cccgagacca
     3541 agcccaagct ggagtacggc accgaggtgt tccagctgga cgaagcgctg ctgcagcgcg
     3601 cgctggcgct gtcgcgtggc gcgccgctga aggacaacga caccgccgcc gcgtaccgca
     3661 ggcaataccc gcaggcggcc gcccaggccg cgccgggcgt gctgcaatgc gacacgctgg
     3721 ccggcgacac ctggtggcac ggcgaccgcc tgggcgagcg cgccacccgc tggacgcgcc
     3781 tgctgaccga cggcaagggc acgtactgca cgacgcagca ggaggacaac gccacctacg
     3841 aggcgctgcg ccgcggcgcg gccgccgggc gcctcgacct caagcgcgtg gcggtgctgc
     3901 gcacgggcgc gaacttcgat cgcccccatc cggggcagac ggaccaggaa tcgctcaacg
     3961 cacgatccgg cggctttccc atcgccactg ccaatctcta cgaggcggcg tggccgctgg
     4021 tgtcggacat cgtggcgcgc tggccgcagt ggcgcgaggg cgtgccggcg agatgaggac
     4081 gatcgcccgc gcgcggggac gcgcgcgagg cggccaggcg ttccgcggtg cacttgcggc
     4141 gcgagggtcg tgcgcgatgc actggaatcg ggcgcatgcg gccgggtccg tcggcggcgc
     4201 gggtcatggc gccgggcgcg catcggctgc catcgcgtca cgccgccacg gctcgtgtga
     4261 cgaccgcgtg acgtcgcgtg cgctgtgcgg cgcaatcgcc tgaacgcggg agtggcgccg
     4321 gattcacacc catcctgaat ccccacgtgg ccttcgtctt gctttacggt gcgcgcacaa
     4381 aaattttcgg gaatccatcc gatgaccact cgcaccacga tccgcccggc catgcgaccc
     4441 accatgccgc tcaagccgtt gtccctggtc ctcctgctcg cgttcgcgcg cgccgcgcac
     4501 gcccaggcgg ccgcgccggc cgacgccgcg tccgccgcgg aggccggcaa gctgcagacg
     4561 atcaccgtca ccgcgcagcg catcaccgag gacgtgcgcc aggtgccgat ctcggtcagc
     4621 accctcggcg ccgacgcgct ggacgtgttc ggttcatcgg gcgacgacat ccgcgcgctg
     4681 tcgggccgcg tgcccagcct caacatcgag tcctcgttcg gccgtgcctt cccgcgcttc
     4741 tacatccgcg gctacggcaa caccgacttc cacctgaacg cgtcgcaacc cgtctcgctg
     4801 gtctacgacg acgtcgtgca ggagaacccg atcctcaagg gcttcccgat cttcgacacg
     4861 gagcaggtcg aggtgctggc cgggccgcag ggcacgctgt tcggccgcaa cacgccggcg
     4921 ggcgtcgtga aggtcgactc ggtggcgccg agccgggtcg ccggcggcta cgccagcgcg
     4981 accttcggca acttccacac gatcaacctc gagggcgcct acggcatgcc gctgggcacg
     5041 gactgggcca tccgcgcctc cacgcagttg cagcatcgcg gcgactgggt ggccaacacg
     5101 gcgcccacgg agcagaccgg caggctggag ggctacgacg acgacgcgct gcgcgtccag
     5161 gcgctctaca agcccacggc cacgttcagc gcgctgttca acgtgcatgg ccgcgacctg
     5221 cacggcaccg cgcgcctgtt ccgcgccaac atcatcaagc ccggcaccaa cgacctggtc
     5281 gacgggttcg acccctatgc ggtgtcgttc gacggccaga accgccagtc gctcaacacc
     5341 agcggcgcca gcgccaagct gcgctgggcg ctcggcgact acgtgctgaa ttcgatcacg
     5401 ggctgggaac acgtgcaggc cttcagccgc ggcgacgtcg acggcggcta cggcgcctcg
     5461 tatgcgccgc cgttcggacc gggcttcatc ccgttctccg acgagacctc cgacgcgctc
     5521 aagggccact cccaggtgag ccaggagttc cgtgtcgaac atgccgattc gagcccgctg
     5581 tcatggcagg ccggcctgta cctgttccgc gagaagtatg cgatcgacag cctcagctac
     5641 gacacgctgt tcggcgcgcc gcccacggag gtcgacgcca cgcaagtcaa caacgcctgg
     5701 gccgtgttcg gctcggccgg gtaccagctg accgccgact tcaagctgcg cgccggcgtg
     5761 cgctggacgc aggatcgcaa gtcgctcgtg acgatcccga tcagcgacac gaaggtcaac
     5821 gccgccaagg gcctggccgc gggcaccagc gactccaaga cgagctggga cctgagcggc
     5881 acctacgcgc tctccaagga cgtcaacgtc tacgcccgcg tcgccacggg cttccgtggc
     5941 tccagcatct tcccggcctc cgacttcaac gtgctgaccg ccgcgaagcc ggagacgacg
     6001 acctcgtacg agttcggcac caaggccgac ctgttcgaca ggcgggcgcg catctcggcc
     6061 gacgtcttcc actacgaggt caagaaccag cagctgagcg cggtcggcgg cgacggcaat
     6121 ggcaccgagc tgctgaacgc caagaaggtc atcggccagg gcctggagct caacctcgac
     6181 gcctacctga cgcccagcct gctggtgacc ttcaacggca gctacaactt cacgcggatc
     6241 aaggacccgt cgctgtcggt ggcgccttgc ggctcctgca cggtgacgga tccgctcgat
     6301 gccaacgggc acgcgctgat cgacggcaac ccgctgccgc aggcgcccaa gtatgtgttg
     6361 aacctgaacg cgcgctacgg catcccggtg ggcaacggcg aatacttcgt ctacacggac
     6421 tggtcgtacc gcagcgaggt caacttcttc ctgtacgagt ccaaggagtt caccggcaag
     6481 gcgatgacca tcggcggcct gcgcgcgggc tacgactgga gcgacggcaa gtacgaggtg
     6541 gccggcttcg tgcgcaacct caccaaccag atccgcgtca cgggcggcat cgacttcaac
     6601 aacctcacgg gcttcatcaa cgacccacgc acctacggcg tgcagttcaa ggcctcgttc
     6661 taagatcgcc ggcagctgcg gccgcgcggg tcgggtgccc gccgcggccg cttttcattt
     6721 ttcgggagat cgacgtgaag aaactgttcg tcgccgccgt cctggccgcg ggcctgtcgt
     6781 cggcgttcgc ggccaggccg gccgccaagg ccgcctgcct gaccgagtgc acgccgcgca
     6841 tcggcatcgt ctcggccttc ggccaggagg cggccgtcct cgtcgcgcag acccgctcgc
     6901 cgcacacctg gatcatcaac ggcaaccggt tcaccaccgg catgctgcgc ggcgagccgg
     6961 tggtgatcgt gctgagcggc gtgagcatgg tcaattcggc catggtcacg cagctgatgg
     7021 tcgaccactt caaggtgggc cgcctggtga tgtcgggcat cgccggcggg gtcgatccgg
     7081 cccaccacgt cggcgacgtc atgattcccg accgctgggc gatgcccctc gaggtgttct
     7141 ggaaccgcga caacgtcctg cccgcgacct gcggcaagga ggcggacgtg tcgtgcctgg
     7201 gcctgaagct ggccagcgtc gacggcaagc cggtgccgcc gttctacctg gccacgcccg
     7261 ccgcgccgat gcccagcggc ctgttcatgc gcgagaactt cgtgatgacg gcggccaacg
     7321 cgccggccgg ggagttccgt ttcgactacc cggtggacgc ggcgatgctg gccgtcgccc
     7381 gcgagatccg gcccgcgctg gcgcgctgcg gaccgaaggc cgtgaagtcg cccggcgccg
     7441 agcccgaccc cgcgctgtgc gtgaagacga ccccgcaggt gatcgtgggc ggccgcggcg
     7501 tctcgggttc ggccttcctc gccaacccgc agtaccggcg ctacctgttc gagcacctgc
     7561 aggcgcaggt cttcgagatg gagacggcgg cgctcgccca cgtcgcctac gccaacggca
     7621 tcccctacat cgccttccgc agcctgagcg acctggccgg cgccgaggaa ttcgaccccg
     7681 atgcggtggc gctgttctcg agcggactgg ccgagaccaa cgaggccgcc gtcacgctgg
     7741 cgttcctgga cggctggcac caccggcgcc gttgagccgc gcgcggctgt aaggaaccgc
     7801 gggggcttcg cgacgaacga aatggccttc ctgcacccgg ggattgcggc atacggcgca
     7861 ccgtatcgaa agtcgtcgat cggtgtaagt gaacgagggt tcggtcgacc aacggaggtc
     7921 gcggccacag ccggccgcgg cgcgcggctc cggccaggag cgcgccacat cacctccagg
     7981 agagaccatg accttcgaac gaatcctgac ggccgccgcg gcggccttcg ttctggccgg
     8041 cgcggccacg accgcatccg ccgaatcgtc cggccagcac acgctggtcc tgaccacgtc
     8101 caacgcgacg accaacgagc tggtggtgct cgacaccgcc ggcaacgtgc tccggcgcct
     8161 gcccacgcag gggcagggcg gcgtggccgg caacgcgggc ggcatcgccg cgagccgcga
     8221 ccgcgtcgct gtcgtcaact tcggctcgtc caacgtctcg gtgttcacgc gtgacgatgg
     8281 cggccgcctg ttccgcctgg agcgcgtgat cccgaccgtg ggcagcccgg tcagcgtcgc
     8341 cttcggccac gaccacctct acgtgctgac caccaccggc gtggagtcgc accgcgcggg
     8401 ctcgtggggc gtgtcgccgg ccgcggacgg ccgatcggca ctgctgcacg ccgacggcag
     8461 cgcggcgcag gtgggcgtgc tgaacgacga gctcgtgttc agcgagaaga gcaacgcgat
     8521 cgagacggcg cggctcgcgg gtgacggcgc ggtggtgggc gcggcggcgc tggtggccgc
     8581 catcccggcc aacgtcgacg cgccgttcgg cctggccgcg cgcggcgacg acgcgtacgt
     8641 gacgatcgcc cacgccgacg agatcagcct cgtgcgccac gacacggtgc tcgcgacgac
     8701 cccctcgatg acgcagcacg cgccgtgctg ggtcgcgctg gacggcccgt tcctgttctc
     8761 ggccaactcg cccagcaaga gcgtgtcgcg ctacgccgtc tacggccaga aggtcgtgca
     8821 ggacgcgccc gtggtggccc agttcgccgg cagcccgacc gacatcgcct accgcgccgg
     8881 ctttgccgcc gtggtcgacg cgggggccgg gctgtcgcgc gtgtcgatct tcaaggtcga
     8941 cgaggacggc aacttcacgc tgcgcggcgt ggccgacctg ggggcagccc acaccaacgg
     9001 cgtggcgatc gtcgacggcg acgcctggcg ctgatctgcg ccaagacccc gcggccgccg
     9061 gcatgtcacg tgccggcgta ccatcggcgc ccggcgtgcg atccgcgccg gcggacttga
     9121 gaagaggcgt ccttggcccg acatggcagg gacgtcgtct aaggagaaat cgagatgccc
     9181 gagtacagga agactcccga ggcgctggcc cggctgacgc cgatccaacg ccatgtcacg
     9241 caggacagcg gcaccgagcg ccccggcacg ggcgagctgc tgcacaacaa ggaggccggc
     9301 atctacgtcg acgtcgtctc gggcgagccg ctgttcgcgt cgtccgacaa gttcgagtcg
     9361 ggctgcggct ggcccagctt caccaagccc atcacgcagg gcttcgtgga cgagttccgc
     9421 gacaccacgc acggcatgat ccgcaccgag gtgcgctcgc accacggcga cagccacctg
     9481 gggcacgtgt tcccggatgg cccgcgcgac cgcggcggac tgcgctactg catcaactcg
     9541 gcctcgctgc gcttcgtgcc gcgcgagcag atggaagcgc agggctatgg cgaatacctg
     9601 ccgcaggtgg aggacgtgca atgacagatt ccttgtcaac ctgttcgcgg gacgagcgcc
     9661 gggccgcccc aagccggccc gcggtcccct cgggggacgg agcgacgacc tcgtcgcggg
     9721 aggggctcca atgaccaccg aacgtgcggt actggccggc ggctgcttct ggggcatgca
     9781 ggaactgatc cgccacaagc cgggcgtgat cgagacccgt gtcggctaca ccggcggcga
     9841 cgtcgaggac gccacgtacc gccgccacgg cacgcacgcc gagggcatcg agatcatctt
     9901 cgacccggcc gtcatcagct accgcgacct gctggagttc ttcttccaga tccacgatcc
     9961 gagcacgaag aaccgccagg gcaacgacgt cggcatgagc tatcgctcgg cgatctacta
    10021 caccacgccc gaacagaagg ccgtggccga ggagacgatc cgcgacgtcg atgcgtcggg
    10081 cctgtggcct ggcaaggtgg tcaccgagct ggagccggcc ggcgcgttct gggaggccga
    10141 gcccgaacac caggactacc tgcagatgtt ccccaacggc tacacctgcc actttccgcg
    10201 gccgggctgg aagctgccca ggcgcaacgg ctgaaacgcc gcgggacggc ccgcccggcg
    10261 ctcagcgcgc ggacgggctg ttgaccaggc cgttggccgc ggcccactgc gcgacgtcgt
    10321 cgcgccggat ggccgccgcc atcagcgccg ggaaggcgtc gggcgtgcat gcgaaggacg
    10381 gcatgccgag cgccgccagc ttggccgcca gcgcgtggtc gtaggccggg cggccctcgt
    10441 cgctgagcgc cagcagcgtg atgcactgga cgccgctgtc gaccagcgcg gacgcgcggc
    10501 gcagcaggtt ggcctcgacg ccgccttcgt agaggtcgga gatcagcacg aagatcgtgt
    10561 tgcgcggctc gcggaccagc gactggcagt agccgaccgc gccgtcgatg tcggtgccac
    10621 cgcccagctg cacgccgaac agcagttcca ccgggtcgtc gagctggtcg gacatgtcga
    10681 ccaccgcggt gtcgaacacc accagcttgg tcgagacggc cggcagcgag gccatcaccg
    10741 cgccgaagat gctggagtac acgaccgagt tggccatcga gccgctctgg tcgacgcaca
    10801 ggatgatctc gcgctgcggc cggcgcgcct tgcggccgta gccgtgcagc gtctcgggca
    10861 cgatggtgcg gtactcgggc tgccagtgct tcaggttggc gcggatggtg cggttccagt
    10921 cgatctgcgc gtgccgtggc cggcggttgc gctggctccg gtcgagggcg ccggcgatgg
    10981 ccgatcgcat cggttcctcc agccgcttca tcaggtcgtc caccacctgc ctgaccacca
    11041 tgcgggcggt gtcgcgcgtg ccggcgggga tgacgcgcga cagggcgatc aggctggcga
    11101 ccaggtgcac gtcgggctgg gcgctgcgca gcatctcggg ctgcagcagc atctcgcgca
    11161 ggttcaggcg ctgcagcgcg tcgtgctgca tcacctgcac gacggaactc gggaagtact
    11221 tgcggatgtc gcccagccag cgcgcgacgc tgggctgcga ggcgccgcgt ccgccgcggg
    11281 tgccgccgcc caggccgccg gccgagccct gcacgtcgta cagcgcggtg agcgcctggt
    11341 ccatctcgat ggcctggccg gccagcgcgc cgcaggccgc ctcggcctcg ctgccgatca
    11401 ccaggcgcca gcgcgccagg cgctcgcgtt cgctgctgct cacggcgggc ctccgttcgt
    11461 gtggggttcg ggcaggccga gcagcgtgcg cagcatcggc agcggcagtg cggcgcgctc
    11521 caggtcccag gcggcgaaca gctcgccgtg cgcgccggcc gggcccgcgg ctgcgtgcgc
    11581 cgcaccgggc ttgtggcgcg ccttctgcga caggtcgcgc agttcggtcg cggcgaaggc
    11641 cgagaaggtg cggcgcacca gcggcagcac gcggatgaag tggtcgtcgc tgagcccggc
    11701 gagccagtcg tcgaccagcg accacacggc ggcgtcgtgc agcagcacca cggcattgcg
    11761 gttcaggaag ccctccagcc aggcggcggc gtgcgcgggc tcggcgccgg cggacaggtt
    11821 cagggacatc gcggtcgccg ccttgtcggc cggccactcg ccggcgtcca ggagcagccg
    11881 cgccgcgacg cccttgagca ggtcatgcgc gccggccagg tcggcgacgt gcgccagagc
    11941 acggcgccag gcctgcgtcg tctccgcgcc gtcgcgcagc gcgatcgcgg cctgggcgga
    12001 caggacgacg tcgcgcaacg attccgcggc gccatcgtcc aggccgacgc aggccaccgg
    12061 caggccgatg gccgcccgca cggccaggcc gtccagcacg tgcgccacga gcgcagcgtc
    12121 ggtctggcgc acgttgccgt agcgaaagac gttggccagc ggcggcagcg tggccaggag
    12181 ctgcgcggcg tcgccggtga tcgcggcgcg gtcctcgagc gcctgcgtgg cggcagcgac
    12241 ggcccgatcg aggtcggcga gcagcgcctg gtcgaccagc gtcgacaacg ccgccagcgt
    12301 gccggcttcg gcgcagcgcg cctgcgtccg cgcggtggca gcctgttcga ccgtctgtcc
    12361 ccacaggctg gcctcgatca ggcggatggc gaattcgggc tgccactgca gcgtccagac
    12421 ctcgtggaag gtgccgcgcg ccgagcgacc gaccttggcg agcgtgcccc agggcacgtc
    12481 cagcagcgcc agccggtgca gcaggtggct gcgggcgagg tcgttgtcgc tgcgcaggtc
    12541 cagatccagc gtctttcgca gcgcctcggg cttcatgcgc aggcgcttct gcagcgcgtc
    12601 gacgtcgcgc tgcagcggca cggtgggcac cgccggcggg acgctgccca ggtggtctcc
    12661 gacgaccagc gcgtcgcgga tgaactgcag ggccgcatcg tcgcccagcg tcagcaccgt
    12721 gcggctcgcc tcgtccagct cgggcaggcc cggggcgggg cgatcgcgca gcgcggcgag
    12781 ggtgtccgcg aggcgggtgg cctcgatcag gtgggccgac gagcagtcga gctcgcgctc
    12841 gcgcatgagg cgcgcgacac gcgcgagcca gcccaccgca cggcgtgcag ggcggtcgtg
    12901 ggtgcgccac aggtgttcgt accagccggg cgaggcgatg cccgcgccgt agccgctggc
    12961 acgcgtgagg tggcgatagg tccacggcac ccaggtcgac tgcaccttga gcctgggcag
    13021 gcccttgagc agggccgcat cggccttggc cgcgaccgtg gcgccgagcg ccggcacgtg
    13081 ccaggcgccg cagacgaccg cgatgcgcgc gaagccttgc ttccgtgccg cgcgcacgca
    13141 ctggcgcatg tgggcctcgc gcagcgcgtc gcggcgcgcg agctcgggcg agaggtggtc
    13201 ctcgccgagc tcggcgcgca gcgacgccat cgccgtgccg atcgcctcga acagctcggc
    13261 gccgtcggcg cgttcctcga ccatgtggtt ccaccacgac tcgccgtcgc cgtagccggc
    13321 cgcctgcgcg agccagctga gcgggtcgcg gcgcccgagg ggttcggccg cgtcctgccc
    13381 ggggtcgggc gccggggcag cgatgggcgg cggcgcggcc tcggcgtccg gagcttcgga
    13441 tggcacggtg ccgccctcgg ccttcgaggc ggcctcgcgt tccgccttcg cctgggcgag
    13501 ctcgtgggcc agcggcaggt cgatgaaacg tgcc
//
LOCUS       DATCRO010000897         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_831680, whole genome shotgun sequence.
ACCESSION   DATCRO010000897 DATCRO010000000
VERSION     DATCRO010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831680"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VIN75_24400"
     CDS             <1..180
                     /locus_tag="VIN75_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977377.1"
                     /translation="VQRITRSIARRRRSRFDASPFGQKTAFASGSYMSNPLGLLYAVH
                     CDGDTAIAPLRRAVE"
     gene            244..615
                     /locus_tag="VIN75_24405"
     CDS             244..615
                     /locus_tag="VIN75_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977378.1"
                     /translation="MNPLTKLAGVAIGAASVVAVVAVAQPNTGTESTMNPATANYGTP
                     PGAPLKRDGSLSASGVESGVVGTTQAPVTTTSTTSSTQSTTTPAAPAPVVNNDQSTAA
                     STTETSTAGTSPALSPRADRN"
     gene            818..1885
                     /locus_tag="VIN75_24410"
     CDS             818..1885
                     /locus_tag="VIN75_24410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8977379.1"
                     /translation="MPTDVSDLPLVSVVIPAFNAAATLPATLDSVLAQTYPHIEIIVV
                     DDGSRDGTPDVLARYAPRVRGLRQANGGLAAARNAGLAAARGELVALLDADDLCEPER
                     IGAQAALMMARPDVVLSGTEFSCFDDTGVVAERFARQYYSRLGRASSGPAAFFGEQAS
                     VDIGAWLPPPSAAQVDMPVLVGDVYRYLALGNFIHPPTVMFRRGLLRRIGVFDRAIPN
                     CCDWEWLVRASRAGKFAFVDRPLLRYRRSMSQMSGPRHKLQLYSDVLANLRQFAQDDP
                     QLPRWGGRDYRRALGTASLNLAGALVETDRLRALGLLAQAAFLGVLERNTARHLAKAL
                     APSSLVHYIQARRGAIPGARS"
     gene            complement(1893..>2717)
                     /locus_tag="VIN75_24415"
     CDS             complement(1893..>2717)
                     /locus_tag="VIN75_24415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977380.1"
                     /translation="AEFNDVLHLPGHADAVATEFVQLLRDRAWGRLLLSGHEPDSVVG
                     RALARVGGTQTEQRCQPAPFVDLQALGDKPFETTLTGKSGTNLRRNRRAYEEELGPIE
                     VRRATGVDEALRFFREMRELHLARWTDLDRSTSLAADAVVDFHERVIRTLVPAGGADL
                     LRVGNAGRAVGFLYNFVVHGKVAMFQSGFHYEPSSRRSPGLLTHALAIEHYRRRGLRE
                     YDFLCGDAQYKRTLCNGTRELMWTTVYRDRPWIRMLLAARRLHQRMNRPADLRQAA"
BASE COUNT          385 a         1006 c          931 g          395 t
ORIGIN      
        1 gttcaacgca tcacaagaag catcgcgcga cgccgtcgca gtcggtttga tgcgtcaccg
       61 ttcggccaga aaacggcgtt cgcgagcggc agctacatga gtaacccgct cggactgctt
      121 tacgccgtgc actgcgatgg ggatacggcg atcgccccat tgcgccgagc cgtcgagtag
      181 ccgacggctc cgggcaccgc ctttgccact ttcgtcgacg tccagacaac caggagacct
      241 cagatgaatc ccctcaccaa actcgccggt gtcgcgatcg gtgccgcgag cgtcgttgcc
      301 gtcgtcgccg ttgcccagcc gaacacgggc accgagtcca cgatgaatcc cgccaccgcg
      361 aactacggca cgccgcccgg agcgccgctg aagcgcgacg gcagcctgtc ggccagcggg
      421 gtcgaatcgg gcgtcgtggg caccacgcag gcgcccgtca ccacgacgag caccacgtcc
      481 tcgacgcagt cgacgacgac ccctgccgcg cccgcgccgg tggtgaacaa cgaccagagc
      541 accgccgcga gcaccaccga gaccagcacg gccggcacca gccccgcgct gagcccgcgc
      601 gccgaccgca actgaccgtc gctgcccccg gcatcggaaa ggccgctgtc cgcagcggcc
      661 ttttgcatgt ccgtccgtct cccccggatc ggtgaatctt gcgtcaacct gaacccagct
      721 gaaccagctc ttcagtatca ttttcggacg cctgcgcgac ggggacctga ccgtcctcgc
      781 gccccgcgag cccgcctcgc ctccctgatc gcccgccgtg ccgaccgacg tctccgacct
      841 cccgctggtg agcgtcgtca tccccgcctt caacgccgcg gccaccttgc cggcgacgct
      901 ggacagcgtg ctggcgcaga cgtatccgca catcgagatc atcgtcgtcg acgacggctc
      961 gcgcgacggc acgcccgacg tcctcgcgcg ctacgcgccg cgcgtgcgcg gcctgcgcca
     1021 ggccaacggc ggcctggctg ccgcgcgcaa cgcgggcctg gccgcggcgc gcggcgagct
     1081 cgtcgcgctg ctggatgcgg acgacctgtg cgagcccgag cgcatcggcg cccaggcggc
     1141 gctgatgatg gcgcgtccgg atgtcgtcct gagcggcacc gagttcagct gcttcgacga
     1201 caccggcgtc gtcgccgagc gcttcgcgcg ccagtactac tcgcgcctcg gacgcgcgtc
     1261 gagcgggccg gcggcgttct tcggcgaaca agccagcgtg gacatcgggg cctggctgcc
     1321 gccgccgtcc gcggcccagg tcgacatgcc ggtgctggtg ggcgacgtct atcgctatct
     1381 ggcgctgggc aacttcatcc atccgcccac cgtcatgttc cgccgcgggc tgctgcggcg
     1441 catcggcgtc ttcgatcgcg cgatcccgaa ctgctgcgac tgggaatggc tggtgcgcgc
     1501 gtcgcgcgcg ggcaagttcg cgttcgtcga ccggccgctg ctgcgctatc ggcgctcgat
     1561 gtcgcagatg tccgggccgc ggcacaagct gcagctctat tcggacgtgc tcgccaacct
     1621 gcgccagttc gcgcaggacg acccgcagct cccgcgctgg ggcggtcgcg actaccgccg
     1681 cgcgctgggc accgcctcgc tgaacctggc gggggcgctc gtcgagaccg accggctgcg
     1741 tgcgctgggc ctgctggcgc aggcggcatt cctcggggtg ctcgagcgaa acacggcacg
     1801 ccatctcgcc aaggcgctgg cgccgtcgtc gctggtgcac tacatccagg cccgccgcgg
     1861 cgcgatcccg ggcgcgcgct cctgagcgcg gatcaggccg cttggcgcag gtcggccggc
     1921 cggttcatcc gctggtgcag tcggcgcgcc gcgagcaaca tgcggatcca cggacgatcg
     1981 cgatagacgg tcgtccacat cagctcgcgc gtgccgttgc acagcgtgcg cttgtactgg
     2041 gcgtcgccgc acaggaagtc gtactcgcgc aggccgcggc ggcggtagtg ctcgatggcg
     2101 agcgcgtgcg tgagcaggcc ggggctgcgc cgcgacgagg gctcgtagtg gaagccgctc
     2161 tggaacatgg ccaccttgcc gtgcaccacg aagttgtaca ggaagccgac cgcgcggccg
     2221 gcgttgccca cgcgcagcag gtcggcgccg ccggccggca ccagcgtgcg gatcacgcgc
     2281 tcgtggaagt cgacgacggc atcggcggcc agcgacgtgc tgcggtccag gtcggtccag
     2341 cgggccaggt gcagctcgcg catctcgcga aagaagcgca gcgcctcgtc gacgccggtc
     2401 gcgcgccgca cctcgatcgg gcccagttcc tcttcgtagg cgcggcggtt gcggcgcagg
     2461 ttcgtgccgg acttgccggt gagcgtggtc tcgaacggct tgtcgccgag cgcctggaga
     2521 tcgacgaagg gcgccggctg gcagcgctgc tcggtctgcg tgccgcctac ccgcgccagc
     2581 gcgcggccga cgacgctgtc gggctcatgc ccggacagca gcagccgtcc ccacgcgcga
     2641 tcgcgcagca actgcacgaa ctcggtggcg accgcatcgg cgtggcccgg caggtgcagc
     2701 acgtcgttga actcggc
//
LOCUS       DATCRO010000898         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_887823, whole genome shotgun sequence.
ACCESSION   DATCRO010000898 DATCRO010000000
VERSION     DATCRO010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887823"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VIN75_24420"
     CDS             <1..523
                     /locus_tag="VIN75_24420"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977381.1"
                     /translation="TYMTRDTEDPLYKQNELVSRLAMMLGGREAELLVLDSVSSGASD
                     DLKRASELAIEMVGSLGFSETFGLLSVGGIPDKLLGPDIQAAVLREARTLLEQAQETC
                     RTLLKAQRPRLDAMAQALLTREVLQGDELKQMLGDVTEGRQPAPTPAQIAAAPVLELA
                     FTRRDDSSSSPKA"
     gene            complement(483..1082)
                     /locus_tag="VIN75_24425"
     CDS             complement(483..1082)
                     /locus_tag="VIN75_24425"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HEY8977382.1"
                     /translation="MTSLRMRHAALRSSLAFAVASIALTATRAAPPAKPAAPVAASSV
                     DAAAPSKTLTDACPAQLPVAQTVTEALAGWTALNQQGNYPFVRVAIYPGPPAETGLIV
                     PTVEYRGQAGLHDRWDLPRRSGGYWMTCAYGNTTATVARKLADDVDFCMADYDGRFLT
                     LVVKHWSCGVKRTMPPPAWERPADRKVKPSAKTTNRRGE"
     gene            1231..1533
                     /locus_tag="VIN75_24430"
     CDS             1231..1533
                     /locus_tag="VIN75_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HEY8977383.1"
                     /translation="MKLEDDDYPPEKLAALRTVRKLVEFWQITDQELLGPLPPPPPPP
                     AASTLPIRYRHPLSGETWDGRGEHPPWLRAALLQEGYTVDQLKRAVTQGADDDAGG"
     gene            complement(1587..2480)
                     /locus_tag="VIN75_24435"
     CDS             complement(1587..2480)
                     /locus_tag="VIN75_24435"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8977384.1"
                     /translation="MDDAILAPLRTAAAAVSDIGLAAMTGALATPALLHDASSGWAAR
                     RARRCRLLFTASATTALIANLAWMWLQAISMTESPPFAALLGAADIVTGTGLGRAWVV
                     ATLALAAGLALASACRYRAFPLRALGVVLAVVATAQACMGHAAADGIGWLVPTMAVHR
                     LATGLWAGSVFAAALVVLNGSPDGMDVVRHARRLSTLATAALVGVVLTGAASAWHALG
                     GSLAPLAPSTASPWSFALEVKLALVAAAIALGGFNRFVVMPSLPGAWPRFARVLRVEA
                     VLLLAVLAVAAWLAGGEPPAM"
     gene            complement(2496..2858)
                     /locus_tag="VIN75_24440"
     CDS             complement(2496..2858)
                     /locus_tag="VIN75_24440"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEY8977385.1"
                     /translation="MTTPLRLALATLAATLSPLAFAHASLVDSFPAKGQTLTGSPAEI
                     HLTFNEHVEPRYCRIKLVSDAGKNFDADRPSADKTRPNAIVAAVPVLKPGTYSARWTA
                     VGSDGHKTHGDFKFTVAK"
     gene            complement(2974..4341)
                     /locus_tag="VIN75_24445"
     CDS             complement(2974..4341)
                     /locus_tag="VIN75_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEY8977386.1"
                     /translation="MLRSAGATIAATTVSRAALAGLPELASHDSPETAPPLQPAGGPH
                     YNPVVTLNGWTLPWRMKDGVKEFHLVAEPVVREMAPGFTARLWGYNGQSPGPTIEVVE
                     GDRVRLFVTNRLPERTSMHWHGQRLPNGMDGVTGLTQPPIEPGKTFVYEFVARRAGTF
                     MYHPHADEMVQMAMGMMGSWVTHPRTPNPAIAAVDRDYVFLLNAYDVTPGASLPRTST
                     MTDFNLWSWNSRVFPGIAPLVARQHERVRIRVGNLTMTNHPIHLHGHEMQVTGTDGGP
                     TPPGSRWPEVTVDIAVGQMRQMEFVADAEGDWSLHCHKSHHTMNAMGHDQPNLIGVDQ
                     GDLAGKIGKLVPGYMAMGSAGMGEMTDMPMPGPPNTAPMMGGDGPYGSLEMGGMFSVV
                     KVRRDQAPGDYRDPGWYKPPAGTTAYEWTGEPPPAASHAAHTPPNDVTVKAKKPGMDG
                     MHMEH"
BASE COUNT          606 a         1552 c         1596 g          597 t
ORIGIN      
        1 cacctacatg acgcgcgaca ccgaggatcc gctctacaag cagaacgagc tggtgagccg
       61 gctcgcgatg atgctgggcg gccgcgaggc ggagcttctg gtgctggaca gcgtctccag
      121 cggcgcctcc gacgacctca agcgcgcctc cgagctggcc atcgagatgg tgggctcgct
      181 gggcttttcc gagaccttcg gcctgctgag cgtcggcggc attccggaca agctgctggg
      241 ccccgacatc caggccgccg tgctgcgcga ggcgcgcacg ctgctcgaac aggcgcagga
      301 gacctgccgc acgttgctga aggcgcagcg cccgcgcctc gacgcgatgg cgcaggcgct
      361 gctcacgcgc gaggtgctgc agggcgacga gctcaagcag atgctgggcg acgtgaccga
      421 aggccgccag cccgcgccga cgcccgcgca gatcgccgcc gcgccggtgc tcgaactcgc
      481 gttcactcgc cgcgacgatt cgtcgtcttc gccgaaggct tgaccttcct gtcggccggc
      541 cgctcccagg ccggcggcgg catcgtgcgc ttgacgccgc acgaccagtg cttgacgacc
      601 agggtcagga agcggccgtc gtagtcggcc atgcagaagt cgacgtcgtc ggccagcttg
      661 cgcgccaccg tcgcggtggt gttgccgtag gcgcaggtca tccagtagcc gccgctgcgg
      721 cgcggcaggt cccagcgatc gtgcaggcct gcctggccgc ggtattccac cgtcggcacg
      781 atcaggcccg tctccgccgg cgggcccggg tagatcgcca cgcgaacgaa cggatagttg
      841 ccctgctggt tcagggccgt ccagcccgcg agcgcctcgg tgacggtctg ggcgaccggc
      901 agctgcgccg ggcaggcgtc ggtcagcgtc ttcgacggcg ccgcggcgtc cacggagctc
      961 gcggccacgg gcgccgccgg cttggcgggc ggagcagcgc gcgtcgccgt gagcgcgatc
     1021 gatgcgacgg cgaaggccag cgaggagcgg agtgcggcgt gtctcatgcg gagcgaggtc
     1081 atgggcggat ctatccagca gtctagggcg tcacccaccg tcgagcagga ggcgcgtcga
     1141 cgagtcccct cgcaagggcg ctaccattga ccccacggcg aacgccgctg ccgggcagcg
     1201 gcctgccgca ttgcagggcg cccgcggccg atgaaactcg aagacgacga ctaccccccc
     1261 gagaagctcg cggcgctgcg caccgtgcgc aagctcgtcg agttctggca gatcaccgac
     1321 caggaattgc tcggcccctt gccaccgccg ccgccaccgc ccgcggcgag cacgctgccg
     1381 atccgctatc gccatccgct cagcggcgag acctgggacg gccggggcga gcatccgccg
     1441 tggctgcgcg cggcgttgct ccaggagggc tataccgtcg accagctcaa gcgcgcggtg
     1501 acgcagggtg cggacgacga cgcgggcggg tgaaacgagt ccgcgctcga ttcgtcggac
     1561 aagcgcaggt gccgaggcgc ccgccttcac atggcgggcg gctcgccgcc cgccagccac
     1621 gcggccaccg ccagcacggc cagcagcagc accgcctcca cgcgcagcac gcgcgcgaag
     1681 cgcggccatg cgcccggcag cgacggcatc acgacgaagc gattgaatcc cccgagcgcg
     1741 atcgccgcgg ccaccagcgc gagcttgacc tccagcgcga agctccaggg cgacgccgtc
     1801 gacggcgcga gcggggccag cgatccgccc agcgcgtgcc acgcgctcgc ggcgccggtg
     1861 agcacgacgc ccacgagcgc cgccgtcgcc agcgtggaca gccgccgcgc atggcgcacc
     1921 acgtccatgc catcgggcga tccgttcagc accacgagcg ccgctgcgaa cacgctgccc
     1981 gcccacaggc ccgtcgcgag ccggtgcacg gccatcgtcg gcaccagcca gccgatgccg
     2041 tcggccgccg catggcccat gcaggcctgc gcggtggcga ccaccgccag cacgacgccc
     2101 agcgcgcgca gcgggaacgc gcgatagcgg caggcgctgg ccagcgcgag gccggcagcg
     2161 agcgcgagcg tcgcgaccac ccacgcgcgg cccaggccgg tgcccgtcac gatgtcagcg
     2221 gcccccagga gcgccgcgaa cggcggcgac tccgtcatcg agatcgcctg cagccacatc
     2281 cacgccaggt tggcgatcag cgccgtcgtg gccgacgccg tgaacagcag ccggcagcgc
     2341 cgcgcgcgac gtgcggccca gcccgaggac gcgtcgtgca gcagcgccgg cgtggccagc
     2401 gcgccggtca tcgcggccaa cccgatgtcc gacaccgcgg cggcggcggt gcgcagcggc
     2461 gcgaggatgg cgtcatccat cgctgcggcg agcggtcact tggcgacggt gaacttgaag
     2521 tccccgtgcg tcttgtgccc gtcactgccg acggccgtcc agcgcgcgct gtaggtgccg
     2581 ggcttgagca ccggcaccgc ggcgacgatc gcgttgggcc tggtcttgtc ggcggatggc
     2641 cggtcggcgt cgaaattctt gccggcatcg ctcaccagct tgatgcggca gtagcgcggc
     2701 tcgacgtgct cgttgaacgt caggtggatc tcggccggcg agccggtgag cgtctggccc
     2761 ttggcgggga acgagtcgac gagcgacgcg tgcgcgaagg cgagcgggct gagcgtggcg
     2821 gcgagggtcg ccagcgcgag gcgcagcggg gtggtcatgt gtcctcctgg aggcgatcgg
     2881 gtgtgctgtc ccgggagtcc gctcgcgcga cagaccgtct cgcctgcgcg cgtgcttccg
     2941 tgacagcaca ccgggttggt gtcgcggaca gcctcagtgt tccatgtgca tgccgtccat
     3001 gcccggtttc ttcgccttga ccgtgacgtc attcggggga gtgtgggccg catgcgacgc
     3061 ggccggcggc ggctcgcccg tccactcgta cgcggtggtg ccggcgggcg gcttgtacca
     3121 gcccggatcg cggtagtcgc cgggcgcctg gtcgcgccgc accttgacca cgctgaacat
     3181 gccgcccatc tcgagcgacc cgtagggccc gtcgccgccc atcatcggcg cggtgttggg
     3241 cgggccgggc atgggcatgt cggtcatctc gcccatcccg gccgagccca tggccatgta
     3301 gccgggcacc agctttccga tcttcccggc gagatcgccc tggtcgacgc cgatcaggtt
     3361 gggctggtcg tggcccatcg cgttcatcgt gtggtgcgac ttgtggcagt gcagcgacca
     3421 gtcgccctcg gcatcggcga cgaattccat ctgccgcatc tggccgacgg cgatgtccac
     3481 ggtcacctcg ggccagcgcg agcccggcgg cgtcgggccg ccgtcggtgc cggtgacctg
     3541 catctcgtgg ccgtgcaggt ggatcgggtg gttcgtcatc gtgaggttgc ccacgcggat
     3601 gcgcacccgc tcgtgctggc gcgcgaccag cggcgcgatg cccgggaaga cgcggctgtt
     3661 ccacgaccac aggttgaagt cggtcatcgt cgacgtcctg ggcaggctgg cgcccggcgt
     3721 cacgtcgtag gcgttgagca ggaagacgta gtcgcggtcg accgccgcga tggccgggtt
     3781 gggcgtgcgc gggtgcgtca cccaggaccc catcatgccc atcgccatct gcaccatctc
     3841 gtcggcgtgc gggtggtaca tgaaggtgcc ggcgcgccgg gcgacgaact cgtagacgaa
     3901 ggtcttgccc ggctcgatcg gcggctgcgt caggccggtc acgccgtcca tgccgttggg
     3961 caggcgctgg ccgtgccagt gcatcgacgt gcgctcgggc agccggttcg tgacgaacag
     4021 gcgcacgcgg tcgccctcga ccacctcgat cgtcggtccc ggcgactggc cgttgtagcc
     4081 ccacaggcgg gccgtgaagc cgggcgccat ctcgcgcacg acgggctcgg ccacgagatg
     4141 gaactccttg acgccgtcct tcatgcgcca gggcagcgtc cagccgttga gggtgacgac
     4201 cgggttgtag tgcgggccgc cggccggctg cagcggcggc gcggtctcgg gcgagtcgtg
     4261 cgaggcgagc tcgggcagcc ccgcgagcgc cgcgcgcgag acggtggtgg cggcgatcgt
     4321 ggcgccggcc gagcgcagca ggaagccgcg g
//
LOCUS       DATCRO010000899         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_707561, whole genome shotgun sequence.
ACCESSION   DATCRO010000899 DATCRO010000000
VERSION     DATCRO010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707561"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /locus_tag="VIN75_24450"
     CDS             complement(<1..2297)
                     /locus_tag="VIN75_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HEY8977387.1"
                     /translation="MHPHPHAHDDPQVIRIDRGAGVNRRQVLAAMAASMALANTACSP
                     PMRVQAHPYVDLPEAGGGNLPLYYASAFVRDGFAHGVLVGTQEGRPVKIEGNPSHPSS
                     LGATDVYAQASVLQLWDPDRSTVVRQRLARGGDARPEQTRAPPEVASSSWSAFEAAWR
                     ERAGALADPQGGATLRVLTGPMTSPTERALLAALLAHGPRARWHVHSPLKDGAAADGS
                     WMAFGRVVSQVLHFDRARCVLALAADPFSDGPGAVRHAMDWSQARTAVRDGGAGAQAP
                     ALFAAEATPGLFGARADHRVALSPGRIEGLLWRVAAHWMPEITPADVGLADPAIAAFE
                     QRLVAALREAGPDALVIGGAGLSAEAHALVLALNQRLGAIGRTVTLIDPPDAAPGAGT
                     LADLVDALDAGAVDTLVVLGANPAYDAPAAFDMPHALRRARLLVHAGLHVDETALLAD
                     WHLPLSHAYEQWGDARAHDGSATLLQPAITPLYDTRSVAELLALLAGDPVRDGHALVQ
                     RQWRTGRGGDFDAFWRASLRAGTVADTASPPLEVHAARLPPRLALQAGPATTTMLTAL
                     FVPDPAAHDGSFANNGWLQELPRPFTKLTWDNAALLSPATARRLGVSTGDVVRLQVET
                     RSLEAPAWVLAQQADDVVTLPLGCGRRRAGRVGNGVGFDAGALRAGTALSASASLQKT
                     DRHHAFAVTEHEIDQHGRELLRTVAAGAGLPPDKPHDELYPPFASPGHAWAMVIDLDA
                     CIGCNACTIACQAENNIPVVGKSQVA"
     gene            complement(2335..2982)
                     /locus_tag="VIN75_24455"
     CDS             complement(2335..2982)
                     /locus_tag="VIN75_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8977388.1"
                     /translation="MSAVFSRRAVLVVRLAVLALLGVCVASVLVFVWRARGYEQLGEP
                     IAQPIPFSHKHHVGDDGIDCRYCHTTVETAAHAGLPSTAVCLSCHSQLFRDQPLLALL
                     HRSEASRQPIAWQRVHFLPAFVYFNHGVHVAKGVACVECHGRVDQMPLEWRARPLQMQ
                     WCLDCHRNPAADLRPKSQEFAMDAPLPPPDQAGLLAREAHLESRRRMTDCSTCHR"
     gene            3107..>3344
                     /locus_tag="VIN75_24460"
     CDS             3107..>3344
                     /locus_tag="VIN75_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977389.1"
                     /translation="MRERFRMRQTAQHAGAARAQRENTGSRGARPRQQSFPEHSLSDA
                     LEGRVGCDRAPDVSRRQAHEVLPEVMFRGEILGAA"
BASE COUNT          467 a         1205 c         1265 g          407 t
ORIGIN      
        1 gcgacctgcg acttgcccac caccgggatg ttgttctcgg cctggcacgc gatggtgcag
       61 gcgttgcagc cgatgcaggc gtccaggtcg atcaccatcg cccaggcatg cccggggctg
      121 gcgaacggcg ggtagagctc gtcgtgcggc ttgtcgggcg gcaggcccgc gcccgcggcg
      181 acggttcgca gcagctcgcg gccgtgctgg tcgatctcgt gctccgtcac cgcgaaggcg
      241 tggtggcgat cggtcttctg caggctcgcg gacgcgctca gcgcggtgcc ggcgcgcagg
      301 gctccggcgt cgaagccgac gccgttgccc acgcgtccgg cccggcgcct gccgcagccc
      361 agcggcagcg tgacgacgtc gtcggcctgc tgcgccagca cccaggcggg cgcctcgagc
      421 gagcgcgtct cgacctgcag ccgcaccacg tcgccggtgg acacgcccag gcgccgcgcc
      481 gtggccgggc tcagcagcgc ggcgttgtcc caggtgagct tggtgaacgg ccgtggcagc
      541 tcctgcagcc agccgttgtt ggcgaagctg ccgtcgtgcg cggcggggtc gggcacgaac
      601 agtgcggtca gcatcgtcgt cgtggcgggg ccggcctgca gcgcgaggcg gggcggcagg
      661 cgcgcggcat gcacctccag cggcggcgac gcggtgtcgg cgaccgtccc ggcgcgcagg
      721 ctggcgcgcc agaacgcgtc gaagtcgccg ccgcgtcccg tgcgccattg gcgctgcacc
      781 agcgcgtggc cgtcgcgcac cgggtcgccg gccagcagcg cgagcagctc ggcgacggag
      841 cgcgtgtcat acagcggcgt gatcgccggc tgcagcaggg tggcgctgcc gtcgtgcgcc
      901 cgcgcgtcgc cccattgctc gtaggcgtgc gacagcggca ggtgccagtc ggccagcagg
      961 gccgtctcgt cgacatgcag ccccgcgtgc accagcagcc tggcgcggcg caacgcatgg
     1021 ggcatgtcga acgcggccgg cgcgtcgtag gccgggttgg cccccagcac cacgagcgtg
     1081 tcgacggcgc ccgcgtcgag cgcgtcgacg aggtcggcca gcgtgcccgc gccgggcgcg
     1141 gcgtcgggcg ggtcgatcag cgtgacggtg cgcccgatcg cgcccaggcg ctggttgagc
     1201 gcgagcacca gcgcatgcgc ctcggccgac aacccggcgc cgccgatcac cagcgcgtcg
     1261 gggccggcct cgcgcagcgc ggccaccagg cgctgctcga aggcggcgat cgccgggtcg
     1321 gcaaggccca cgtcggcggg cgtgatctcg ggcatccagt gcgccgcgac gcgccacagc
     1381 agcccctcga tgcgccccgg cgacagcgcc acgcgatggt cggcgcgcgc gccgaacagg
     1441 cccggcgtgg cctccgcggc gaacagcgcc ggcgcctgcg cgcccgcgcc gccatcgcgc
     1501 acggccgtcc gcgcctgcga ccagtccatc gcgtggcgca cggcgcccgg accgtcgctg
     1561 aacggatcgg ccgcgagcgc gagcacgcag cgcgcgcggt cgaagtgcag cacctgcgag
     1621 acgacgcggc cgaaggccat ccatgaaccg tcggcggcgg cgccgtcctt caacggcgaa
     1681 tgcacatgcc agcgtgcgcg cggcccatgc gccagcaacg cggccagcaa ggcgcgctcg
     1741 gtgggcgagg tcatcggtcc ggtcagcacg cgcagcgtcg cgccgccttg cgggtcggcc
     1801 agcgcgccgg cgcgctcgcg ccacgcggcc tcgaacgcgc tccagctcga cgacgcgacc
     1861 tcgggcggcg cccgcgtctg ctcggggcgc gcgtcgccac cgcgcgccag gcgctggcgc
     1921 acgaccgtgg agcggtcggg atcccacagc tgcagcaccg aggcctgcgc gtacacatcc
     1981 gtcgctccca ggctggaggg atgcgacggg ttgccctcga tcttgaccgg ccgcccctcc
     2041 tgcgtgccca ccagcacccc atgcgcgaag ccgtcgcgca cgaaggcgct cgcgtagtac
     2101 agcggcaggt tgccgccgcc ggcctcgggc aggtcgacgt acggatgggc ctgcacgcgc
     2161 atcggcggcg aacacgccgt gttggccagc gccatcgacg cggccatcgc cgccagcacc
     2221 tggcgccggt tgaccccggc gccgcggtcg atgcggatca cctgcgggtc gtcgtgggcg
     2281 tgcgggtggg gatgcatggc gtgggcggga cgaagggcag caagcgacat gccgtcagcg
     2341 atggcaagtg gagcagtcgg tcatgcggcg ccggctctcg aggtgggcct cgcgggccag
     2401 caggccggcc tggtccggcg gcggcagcgg cgcatccatg gcgaactcct gcgacttggg
     2461 ccgcaggtcc gcggccgggt tgcgatggca atcgaggcac cactgcatct gcagcggacg
     2521 cgcgcgccat tcgagcggca tctggtcgac gcggccatgg cattccacgc aggccacgcc
     2581 cttggcgacg tgcacgccgt ggttgaagta gacgaacgcc ggcaggaagt gcacgcgctg
     2641 ccaggcgatc ggctggcggc tggcctcgct ccggtgcagc agcgcgagca gcggctggtc
     2701 gcggaagagc tgcgaatggc agctcaggca gacggcggtg gacggcaggc cggcatgggc
     2761 ggcggtctcc accgtcgtgt ggcagtagcg gcaatcgatg ccgtcgtcgc cgacatggtg
     2821 cttgtggctg aacgggatcg gctgcgcgat cggctcaccg agctgctcgt agccgcgcgc
     2881 acgccagacg aacaccagca cgctggcgac gcacacgccc agcaacgcga gcacggccag
     2941 gcgcacgacg agcaccgcgc gtcgcgagaa gacggcgctc atcgccgacg ccgcggcaag
     3001 gtgccgagcg aaacgaggcg atgtcgcgca agcacggcgg ggcgcgcgcg acggccggca
     3061 atcggcatgc ggtctccttg taggacgccg ccaccgggca aatcgcatgc gcgaacgctt
     3121 caggatgcgc caaaccgcac agcatgcggg cgccgcgcgc gcacagaggg aaaacaccgg
     3181 atcgcgcggc gcccgtcctc gtcaacaatc gttcccggaa cattcgcttt ccgacgcgct
     3241 ggagggcagg gtcggatgcg atcgtgcacc ggacgtctca cgacgccagg ctcacgaagt
     3301 acttcctgag gtgatgttcc ggggtgaaat tttaggggcc gcat
//
LOCUS       DATCRO010000900        18373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_517026, whole genome shotgun sequence.
ACCESSION   DATCRO010000900 DATCRO010000000
VERSION     DATCRO010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517026"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="mlaE"
                     /locus_tag="VIN75_24465"
     CDS             complement(<1..721)
                     /gene="mlaE"
                     /locus_tag="VIN75_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY8977390.1"
                     /translation="MIELVNSLGQSTLRVLAALGHAAYFFVDLLRFTPSSLRRFGLVV
                     DQIYAIGYRSLVIILASGFAVGCVLALQMYYALVPFGAAESLGLVVNLSLVRELGPVV
                     TALLFAGRAGTALTAEIGLMKAGEQLSAMELMAVDPRSRVLAPRFLAGIVSMPILAIL
                     YSAIGIMGAWLIAVQLIGVDPANFWSIMQSKVDVTRDVGNGIVKSAVFGVICTGVALY
                     QGYETQATPEGVAYATTRTVVF"
     gene            complement(718..1527)
                     /locus_tag="VIN75_24470"
     CDS             complement(718..1527)
                     /locus_tag="VIN75_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8977391.1"
                     /translation="MNSDKFIEIDHVTFGYDASRVILNDVSLQFARGKVTAILGGSGC
                     GKTTLLRLIGGVYKPQKGRVMFDGQEVDTRNREQLYALRRRLGMLFQFGALFTDLSVY
                     ENVAFPLREHMDLSESMIRDIVLMKLNAVGLRGAAQLRISQVSGGMARRIALARAVAL
                     DPEVIMYDEPFAGLDPISMGVTANLIRRLNDATGATSLMVSHDVQECFAIADYAYLMM
                     PGGIVVAHGKPEELNRSEDPQVRQFIRGEPDGPIKFHYPAEPLGQALGLPQ"
     gene            complement(1822..3288)
                     /locus_tag="VIN75_24475"
     CDS             complement(1822..3288)
                     /locus_tag="VIN75_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY8977392.1"
                     /translation="MGKVTGFMEFERLEEGYAPVPQRLKHYKEFVIGLKADEAKVQAA
                     RCMDCGTPFCNNGCPVNNVIPDFNDLVYANQWKHASAVLHSTNNFPEFTGRICPAPCE
                     AACVLNVNDDAVGIKSIEHAIADRAWEEGWIAPQKAAVRSGKKVAVVGSGPAGLAAAQ
                     QLARAGHDVTVFEKNDRVGGLLRYGIPDFKMEKTHIDRRVAQMEAEGVLFRTGVVVGD
                     WPKGAKITNHAKESVSAEQLKSEFDAVLLAAGSEVSRDLPVPGRDLDGVHFAMEFLPQ
                     QNKVNAGDKLKGQLTAAGKNVIVIGGGDTGSDCVGTSNRHGARSVTQFELMPMPPEQE
                     NKPLVWPYWPTKLRTSSSHEEGCSREFAIATKEFLGEKGKLTGLKTVQIEFRDGKLAE
                     VAGTEKVWPAELVLLAMGFTNPLAQLLQSFGVDADARGNAKAGVDEKTGYKTSADKVW
                     AAGDVRRGQSLVVWAIREGRQAARAIDLALMGATTLPR"
     gene            complement(3362..8119)
                     /locus_tag="VIN75_24480"
     CDS             complement(3362..8119)
                     /locus_tag="VIN75_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY8977393.1"
                     /translation="MSQAARDQANTPIDIASAAAGGLYDASNEKDACGVGFVAHIKGQ
                     KAHSIVMQGLKILENLDHRGAVGADKLMGDGAGILIQIPDEYFRAEMADQGVELPPPG
                     EYGVGQIFLPKEHASRLACEQELERAVKAEGQVLLGWRDVPVDREMPMSPTVRAKEPV
                     LRQIFIGRGADVIVPDSLERKLYVIRKTASAAIQALKLTHSREYYVPSMSCRTIIYKG
                     LLLADQVGKYYKDLADPRVVSALALVHQRFSTNTFPEWPLAHPYRMVAHNGEINTVKG
                     NFNWMRAREGVMKSPVLGEDLKKLYPISFEGQSDTATFDNTLELLTMAGYPLAHAAMM
                     MIPEAWEQHTVMDPRRRAFYEYHAAMMEPWDGPAAMVFTDGKQIGATLDRNGLRPARY
                     IVTDDDLVVMASESGVLDIPQNKIIKKWRLQPGKMFLIDIEQGRIVDDEELKSQFASA
                     KPYRQWIDNVRVKLDEIPVLAAPASEDAATTLLDRQQAFGYTQEDIKFLLSPMATLGE
                     EGIGSMGNDSPLAVLSDKNKPLYNYFKQLFAQVTNPPIDPIREAIVMSLNSFIGPKPN
                     LLDINAVNPQMRLEVSQPVLDFDDMARLRAIEKHTFGKFKPHEIDIVYPISWGREGVE
                     AKLASLCAEAVDAIRGGHNILIITDRRVGKDSVAIPALLALSAIHQHLVREGLRTTAG
                     LVVETGSAREVHHFAVLAGYGAEAVHPYLALETLASLHAELPGQLSAEKATYNYIKAV
                     GKGLSKIMSKMGVSTYMSYCGAQLFEAIGLDAGVVGKYFRGTASQVGGMGVFEIAEEA
                     LRMHRAAFGDDPLLAEMLDAGGEYAWRTRGEEHMWTPDAIAKLQHSTRSNKFDTYKEY
                     AQIINDQSKRHMTLRGLFEFKFDPSKAIPLEEVEPATEIVRRFATGAMSLGSISTEAH
                     STLAIAMNRIGGKSNTGEGGEDPARYRNEFKGIPIKQGTKVSEVLGDGVMVADFELKE
                     GDSLRSKIKQVASGRFGVTTEYLVSADQIQIKMAQGAKPGEGGQLPGGKVSDYIGKLR
                     YSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPRADISVKLVSEVGVGTIAAGVAK
                     CKADHLVIAGHDGGTGASPWSSIKHAGSPWELGLAETQQTLVLNQLRGRVRVQADGQM
                     KTGRDVVIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRKKFAGK
                     PEHVVNYFFFIAEEARQIMAQLGIRKFDDLIGRSELLDTRKGIAHWKACGLDFSRVFQ
                     QPAMPASVSRLHNDTQDHGLDKALDRKLIERSRPAIDRGEKVQFMEDARNLNRSVGAM
                     LSGELIRARPEGLPDHTLFIQMEGTGGQSFGAFLAQGITLYLIGDSNDYTGKGLSGGR
                     VVIRPSIEFRGDATKNIIAGNTALYGATSGEAYFRGVAGERFAVRLSGASAVVEGTGD
                     HGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVFDEDGRFAERCNTAMVSLDRILPADE
                     QAKSVERAIWHKGEADETLLRQMIEAHHKWTGSLRAREILDHWADSRARFVKVFPNEY
                     KRALGEIHARQEAGATIEKARGKDAKSQTVPAK"
     gene            complement(8548..9255)
                     /locus_tag="VIN75_24485"
     CDS             complement(8548..9255)
                     /locus_tag="VIN75_24485"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8977394.1"
                     /translation="MARLTRLSLSGLPHHLMQIGNNRQPIFVDDEDRQAWCAHLREAS
                     LVARVDIHAYVLMSNHVHLLATPRDSADGLSTMMQSLGRRYVAAFNRRHGRTGTLWEG
                     RFRAALVDPDDSLLACMRYIDSHAQRSGLVAEAADYAWSSCAHHLGRRRDPLIVDHAR
                     YWALGNTPFEREGAWRGFLDEGVSQREAQAISQATRRGWPIAGADGQRRLAAQLGRET
                     VPRPRGRPRRTVVAPAP"
     gene            complement(9315..10502)
                     /locus_tag="VIN75_24490"
     CDS             complement(9315..10502)
                     /locus_tag="VIN75_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY8977395.1"
                     /translation="MIASPLVERPPYASDPARSRGRRVPEAAAPTRTEFQRDRDRIVH
                     STAFRRLVYKTQVFLNHEGDLFRTRLTHSLEVAQLGRSVARTMGLDEDLVEAIALAHD
                     LGHTPFGHAGQDALDDCMHGHGGFEHNLQSLRVVDELELRYPEFDGLNLTLETREGIL
                     KRITLAQARALEAVEPGGVGYRFIAGQQPGLEAQLTNVADEIAYNCHDVDDGIRSGLL
                     DPAQLDDVPLYARLRAEALDAHPRLRGLPPRRTVHETLRRMLSQMIYDLIATSQAAID
                     AAAPASPQAVRELPVLMRFSPAMRHESSELKRFLFANLYRHPAVNATTDRAKEIVRAL
                     FAIYLDRPAEMSDEFQHAVDRPRAVADYIAGMTDRFALKEHQRLTGQVLFGEWASLGR
                     PAG"
     gene            complement(10518..11642)
                     /gene="aroB"
                     /locus_tag="VIN75_24495"
     CDS             complement(10518..11642)
                     /gene="aroB"
                     /locus_tag="VIN75_24495"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY8977396.1"
                     /translation="MSTPSELLRAAPTQVHIDLGERSYPILIGGDLLSDPATWAPQAN
                     RKAVALIVTNVTVAALYGAALAAALEPHFARVRTLALPDGEEYKDLAHLNLVFDELLG
                     QEADRRTVLYALGGGVVGDMTGFAAACYMRGVPFVQVPTTLLAQVDSSVGGKTAVNHP
                     LGKNMIGAFYQPRQVVCDLDVLDTLPDREMSAGLAEIIKYGASIDIEFLGWLEHHVAD
                     LRARKRDALAHAVRRSCEIKAHVVGADEKEAGLREILNFGHTFGHAIEAGLGYGQWLH
                     GEGVGCGMVMAAELSARLGLVEAAFARRIEALVARAGLPVRGPASLSPQRYLDLMRVD
                     KKAQGGDIRFVLLDGQGHASVRTAPEAVVVDVIAALTASA"
     gene            complement(11649..12206)
                     /locus_tag="VIN75_24500"
     CDS             complement(11649..12206)
                     /locus_tag="VIN75_24500"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY8977397.1"
                     /translation="MNQEGRGSPAIALVGMPGCGKSTIGRQLARMRQLPFLDSDHEIE
                     VYLGCSIREFFDREGEAAFREVEERVIRETLADSQRRVIATGGGSVLRATNRAEMKAR
                     ATVVYLHTQVDDLARRLSRDTARPLLQVADPRQRLRELYTVRDPLYREVADIVVDTAH
                     KSAAMLVNLISMQLDMSAGSREDAK"
     gene            complement(12203..13918)
                     /locus_tag="VIN75_24505"
     CDS             complement(12203..13918)
                     /locus_tag="VIN75_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977398.1"
                     /translation="MKLQGIEYLKPLFAAVAVGAVIAGCSAGGGNKPAPTPSPTPAPP
                     AASDLVVSVDKTSIADTGAETATITATAVDANRNVVAGVPVTIVPDRNGVVTTSGSST
                     SATGVVNGVLGIGGDKSNRTIDVVVTSGTITKTVKVDVVGAQLTASAGAATAGQTATV
                     QYQLTDKNNGSIANEPIVITLNGTATNGTTDGNGLYTYSYTVPATATVSLSATAAGVT
                     TQTSVTTSSSSTAAATGTVTSASVAANPATVNVNTAGSTSNFVTVRGLFLGAGNAPIQ
                     YVRVRFDLNGDPSGIGGTLAAGSGYVYTDANGVAQTTYTPGTRGSGNQQLTVRACWSE
                     TDFAAGTCPNAATANVTVAQSGVSISIFNNGKIGNDDTKAVYNLPYTVQVVDSVGQPI
                     KGVLVTGSVDLPRYYRGYWDGSTGAWKYVNLNVCDNEDINRNNVLETYSNSQKEDQNG
                     SGKIEPYAADAAITPQSAGSDTTDAFGLAYFNLQYGQNVAWWDDFTLTFSAQVSGTEG
                     KATTSGRLGVPASLINGATNVSLPFQESPYNLAPPNVAPAAVTTVTVMDPATSRSGNL
                     CQAQP"
     gene            complement(13929..16043)
                     /locus_tag="VIN75_24510"
     CDS             complement(13929..16043)
                     /locus_tag="VIN75_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY8977399.1"
                     /translation="MRSWTAGAALVLALGLPLTSWAEPKITSVTGLQQGGTDVVRVEL
                     SEPLSAVPAGFAVQAPPRVAIDLPGVTNASGKSSVEVNQGNIRSVSLASSGERTRLVL
                     NLKTPSSYHAEIQGKSLLIALDMAQPAAAAAAAQPAAADTHFAASQNADVQELRDVDF
                     RRGTDGAGRIVVNLPSNQVGVDLKQQGKDLVVDFLRSNAPTRLRRRLDVTDFGTPVQT
                     ITTTQAGDHVHMVVTPTGNWEHSAYQTDNQFVLEVRPAKADPNKLVPGTGYTGQRLSL
                     NFQSIEIRALLQIIADFSDFNVVTSDSVTGTVTLRLKDVPWDQALDIILQSKGLDKRR
                     SGNVLLIAPKDELAAKEQVDLEAKKKIADLEPMRMQAFQLNYTKALEVANGLTGTGQS
                     GGAGTRLLSQRGSVIAETRTNQLFVTDIPSKLEEIQALIARIDVAQRQVMIEARIVEA
                     NDNFGRALGVKLGGSAFNAFHLGGSQYVSPGGNYEAVGYQSGQIQGTVPYTDSQFVNF
                     PAQAGIVSSTATAATFALSLFSSSASRFLNLELSALEEDGSGRIISSPRVITADQVQA
                     VVEAGEELPYQQATSSGATSVAFQKAQIRLQVTPQITPEGNVILDVEVNKDSVGRSTS
                     AGFAIDTKHIKTTALVENGGTVVLGGLFQQIENNVTDKVPFLGDIPFLGNLFRSTSKL
                     QTRTELLIFITPRIISDRTQAH"
     gene            complement(16067..16600)
                     /locus_tag="VIN75_24515"
     CDS             complement(16067..16600)
                     /locus_tag="VIN75_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY8977400.1"
                     /translation="MNRLLCITAGALLLSGLGACTADNDELIQWMEQQHKEIKPNIAP
                     IYPPKKFDPQPYLGVTGVEPFGTQKLIPVGGPSNGKGSALLAAAKAHATQELESYPLD
                     SMTMVGSLQQGGKAHALLMVENRLHDVKVGDWIGQNYGQITAITDTQITLRETVQDPT
                     GEWIERTSTLQLQEKAR"
     gene            complement(16597..17274)
                     /locus_tag="VIN75_24520"
     CDS             complement(16597..17274)
                     /locus_tag="VIN75_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY8977401.1"
                     /translation="MATAKNKGPDIGAALRGGLSQFRDLNPNQPGQWPLLPRLSVWAV
                     VAIAVVVIGWFVELSSQADDLQAARDKEPALKAEYKTKLAQAINLDALKKQKEEVQTY
                     VTQLEKQLPGKAEMDALLTDINQAGVGRGLVIDQFIPGATETKEYYAELPIAIRVTGR
                     FHDIGAFAADIAALSRIVTLHNLSISPDQKSAGGGLSMEAVARTYRYLDQAEVVEARK
                     AGAKDKK"
     gene            complement(17277..17936)
                     /locus_tag="VIN75_24525"
     CDS             complement(17277..17936)
                     /locus_tag="VIN75_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY8977402.1"
                     /translation="MILINLLPHREARRQQRKQAFFAGIGFAVLVGAGIVLAWYTFVN
                     TQISTQEEHNQFLSSEIKRLDAQIADVATLKAEIEALKARQKAVEDLQADRNMPVYLL
                     NELVKQTPEGVFLTSVKQAAQQVKITGVAQTNERISEFLRNTASNSPWLEKPQLIEIR
                     AQTMQVAGSKDQRRMFAFSMSVTLKRPPAPGAAASGAAGAASGAAAAGATAPAASTAT
                     R"
     gene            complement(17933..>18373)
                     /gene="pilM"
                     /locus_tag="VIN75_24530"
     CDS             complement(17933..>18373)
                     /gene="pilM"
                     /locus_tag="VIN75_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8977403.1"
                     /translation="LRGDEMLYDRDQTFGGAQLTQLISRQYGFSFEEAEQKKLAGDLP
                     EDFESTLLMPFVDSLAHEIGRALQYFFTSTPHHKVDYVMLAGGTASLSGLRERVKEVT
                     GFACRVVNPFENMKLGSAVREGKLRREAPSYLTACGLAMRRFVQ"
BASE COUNT         2636 a         6109 c         6393 g         3235 t
ORIGIN      
        1 agaacaccac cgtgcgcgtg gtcgcatagg ccacgccctc gggcgtcgcc tgcgtctcat
       61 agccctggta cagggcgact ccggtgcaga tcacgccgaa cacggcggac ttgacgatgc
      121 cgttgcccac gtcgcgcgtg acgtcgacct tggactgcat gatcgaccag aagttggccg
      181 gatccacgcc gatcagctgc acggcgatga gccaggcgcc catgatgccg atcgccgaat
      241 acaggatcgc caggatgggc atcgagacga tgcccgccag gaagcgcggc gccagcacgc
      301 ggctgcgcgg atcgacggcc atcagctcca tcgccgacag ctgctcgccc gccttcatca
      361 ggccgatctc ggcggtcagc gcggtgccgg cgcggcccgc gaacagcagc gcggtgacca
      421 ccgggccgag ctcgcgcacc agcgacaggt tgaccaccag tcccagcgat tcggcggcgc
      481 cgaacggcac cagcgcgtag tacatctgca gcgccagcac gcagccgacg gcaaagccgg
      541 aagccaggat gatgaccagc gagcggtagc cgatggcgta gatctggtcg accacgagcc
      601 cgaaccgccg cagggacgac ggcgtgaagc gcagcaggtc gacgaagaag tacgccgcgt
      661 gcccgagcgc ggcgagcacg cgcagggtcg attgcccgag gctgttgacg agttcgatca
      721 ttgcggcagc cccagcgcct ggccgagcgg ctcggcgggg tagtggaact tgatcgggcc
      781 gtccggctcg ccgcggatga actggcgcac ctgcggatct tccgaccggt tcagctcctc
      841 gggctttccg tgcgccacca cgatgccgcc gggcatcatc aggtaggcgt agtcggcgat
      901 ggcgaagcac tcctgcacgt cgtgcgacac catcagcgag gtggcgccgg tggcgtcgtt
      961 caggcggcgg atcaggttgg ccgtgacgcc catcgagatc gggtcgaggc ccgcgaaagg
     1021 ctcgtcgtac atgatgacct cggggtcgag cgcgaccgcg cgcgcgagcg cgatgcggcg
     1081 cgccatgccg cccgagacct gcgagatgcg cagctgcgcc gcgccgcgca ggccgacggc
     1141 gttgagcttc atcagcacga tgtcgcggat catcgactcg ctcaggtcca tgtgttcgcg
     1201 cagcgggaag gcgacgttct cgtagacgct caggtcggtg aacagcgcgc cgaactggaa
     1261 cagcatgccc aggcggcggc gcagcgcgta cagctgctcg cggttgcgcg tgtcgacctc
     1321 ctggccgtcg aacatcacgc ggcccttctg cggcttgtag acgccgccga tcaggcgcag
     1381 cagcgtcgtc ttgccgcagc ccgatccgcc caggatggcc gtgaccttgc cgcgcgcgaa
     1441 ttgcagcgac acgtcgttca ggatcacgcg actggcgtcg tagccgaagg tgacgtgatc
     1501 gatctcgatg aatttgtcgg agttcaagcc ggcatccggc cgccaggggc ggcgttcagg
     1561 tggaagggga caggcgcgac agcttcgccg caccgacctt tctggatcct gtccaggcgg
     1621 ggggcctaag gggtttcccg ctgccgcatt gtgtgcgaac tccgctgcgc acgttgggaa
     1681 cccccacgga aaacccctaa aagcctgcct aggcaatgat cttcgtcgca tccacgcaac
     1741 gagaccggca accgcgcccg gttacacgac aggaacagga acgggcgccg cagcgcccgt
     1801 ctccgatgcc cgcgcgaccg ttcagcgcgg cagcgtcgtc gcacccatca gggcgaggtc
     1861 gatggcgcgc gcggcctggc ggccctcgcg gatcgcccac accacgagcg actggccgcg
     1921 gcgcacgtcg ccggcggccc agaccttgtc ggcgctggtc ttgtagcccg tcttctcgtc
     1981 gacgccggcc ttggcgttgc cgcgcgcgtc ggcgtcgacg ccgaacgact gcagcagctg
     2041 cgccagcggg ttggtgaagc ccatcgccag cagcacgagc tcggccggcc acaccttctc
     2101 ggtgcccgcg acctctgcca gcttgccgtc cctgaactcg atctgcaccg tcttcaggcc
     2161 cgtgagcttg cccttctcgc ccaggaactc cttggtggcg atggcgaact cgcgcgagca
     2221 gccttcctcg tgcgacgacg aggtgcgcag cttggtcggc cagtacggcc acaccagcgg
     2281 cttgttctcc tgctccggcg gcatgggcat cagctcgaac tgcgtgacgc tcctggcgcc
     2341 atggcggttg ctggtgccca cgcagtcgct gccggtgtcg ccgccgccga tcacgatcac
     2401 gttcttgccc gcggccgtca gctggccctt gagcttgtcg cccgcgttga ccttgttctg
     2461 ctgcggcagg aactccatcg cgaagtgcac gccgtccagg tcgcggcccg gcacgggcag
     2521 gtcgcgcgag acctccgagc cggcggccag cagcacggcg tcgaactcgg acttgagctg
     2581 ctcggcgctc accgactcct tggcgtggtt ggtgatcttg gcgcccttgg gccagtcgcc
     2641 gacgaccacc ccggtgcgga acaggacgcc ctcggcctcc atctgcgcca cgcggcggtc
     2701 gatgtgcgtc ttctccatct tgaagtcggg gatgccgtag cgcagcaggc cgccgacgcg
     2761 gtcgttcttc tcgaacaccg tcacgtcgtg gccggcgcgc gcgagctgct gcgcggcggc
     2821 caggccggcc gggccggaac ccaccaccgc caccttcttg cccgagcgca cggcggcctt
     2881 ctgcggcgcg atccagcctt cttcccaggc gcggtcggcg atcgcgtgct cgatcgactt
     2941 gatgcccacc gcatcgtcat tgacgttgag cacgcacgcg gcctcgcacg gcgccgggca
     3001 gatgcggccg gtgaactcgg ggaagttgtt cgtcgagtgc agcaccgcgg acgcgtgctt
     3061 ccactggttc gcgtagacga ggtcgttgaa gtcggggatg acattgttga ccgggcagcc
     3121 gttgttgcag aacggggtgc cgcagtccat gcagcgcgcg gcctgcacct tggcctcgtc
     3181 cgccttcagc ccgatgacga attccttgta gtgcttcagg cgctgcggca cgggcgcgta
     3241 gccctcctcg aggcgctcga actccatgaa gccggtgact tttcccatga tctcttcctg
     3301 atgctggttg cgacgggccg cgcgctcctc gaacgcggcc cgaacgtttt ctggctggtt
     3361 cttacttggc gggaaccgtt tgcgacttcg cgtccttgcc gcgcgccttc tcgatggtgg
     3421 cgccggcctc ctgcctggcg tggatctcgc cgagcgcgcg cttgtactcg ttcgggaaca
     3481 ccttgacgaa gcgggcgcgc gaatcggccc agtgatccag gatctcgcgg gcgcgcaggc
     3541 tgcccgtcca cttgtggtgc gcctcgatca tctggcgcag cagcgtctcg tcggcttcgc
     3601 ccttgtgcca gatggcgcgc tcgacggact tggcctgctc gtcggccggc aggatcctgt
     3661 cgagcgagac catcgcggtg ttgcagcgct cggcgaacct gccgtcctcg tcgaacacgt
     3721 aggccacgcc gcccgacatg ccggccgcga aattgcggcc cgtcttgccg aggacgacca
     3781 cggtgccgcc ggtcatgtac tcgcagccgt ggtcaccggt gccttcgacg accgccgagg
     3841 cgcccgacag gcgcaccgcg aagcgctcgc ccgccacgcc gcggaagtag gcctctccgc
     3901 tggtggcgcc gtacagcgcc gtgttgccgg cgatgatgtt cttcgtggcg tcgccgcgga
     3961 actcgatcga cggtcggatc acgacgcggc caccggacag gcccttgccg gtgtagtcgt
     4021 tggagtcgcc gatcaggtac agcgtgatgc cctgcgcgag gaacgcgccg aagctctggc
     4081 cgccggtgcc ctccatctgg atgaacagcg tgtggtccgg caagccttcg ggacgcgcgc
     4141 ggatcagctc gcccgacagc atcgcgccga cggagcggtt caggttgcgc gcgtcctcca
     4201 tgaactgcac cttctcgccg cggtcgatgg ccgggcgcga gcgctcgatc agcttgcggt
     4261 cgagggcctt gtccagcccg tggtcctgcg tgtcgttgtg caggcgcgag acgctggccg
     4321 gcatcgccgg ctgctggaac acgcgcgaga aatcgaggcc gcaggccttc cagtgcgcga
     4381 tgcccttgcg cgtgtcgagc agctcgctgc ggccgatcag gtcgtcgaac ttgcggatgc
     4441 ccagctgcgc catgatctgg cgcgcttcct cggcgatgaa gaagaagtag ttgacgacgt
     4501 gctcgggctt gccggcgaac ttcttgcgca gcaccggatc ctgcgtggcc acgcccaccg
     4561 ggcaggtgtt caggtggcac ttgcgcatca tgatgcagcc ctcgacgacc agcggcgcgg
     4621 tcgcgaagcc gaactcgtcg gcgcccagca gcgcgccgat caccacgtcg cggcccgtct
     4681 tcatctggcc gtcggcctgc acgcgcacgc ggccgcgcag ctggttgagc accagcgtct
     4741 gctgcgtctc ggccaggccc agctcccacg gcgagcccgc gtgcttgatc gacgaccacg
     4801 gcgacgcgcc cgtgccgccg tcgtggccgg cgatgaccag gtggtcggcc ttgcacttgg
     4861 ccacgcccgc ggcgatggtg cccacgccca cttcggagac cagcttgacg ctgatgtccg
     4921 cgcgcgggtt ggcgttcttc aggtcgtgga tgagctgagc caggtcctcg atcgagtaga
     4981 tgtcgtggtg cggcggcggc gagatcaggc ccacgcccgg caccgagtag cgcagcttgc
     5041 cgatgtagtc ggacaccttg ccgcccggca gctggccgcc ctcgcccggc ttggcgccct
     5101 gggccatctt gatctggatc tggtcggccg agacgaggta ctcggtggtg acgccgaagc
     5161 ggcccgacgc gacctgcttg atcttgctgc gcaggctgtc gccttccttg agctcgaagt
     5221 cggccaccat gacgccgtcg ccgagcacct cggagacctt ggtgccctgc ttgatcggga
     5281 tgcccttgaa ctcgttgcga tagcgcgccg gatcctcgcc gccctcgccc gtgttgctct
     5341 tgccgccgat gcggttcatc gcgatggcca gggtcgagtg cgcctcggtg gagatcgagc
     5401 cgagcgacat cgcgccggtc gcgaagcgac gcacgatctc ggtggccggc tcgacttcct
     5461 ccagcgggat ggccttgctc gggtcgaact tgaactcgaa caggccgcgc agcgtcatgt
     5521 ggcgcttgct ctggtcgttg atgatctgcg cgtattcctt gtaggtgtcg aacttgttcg
     5581 agcgcgtgct gtgctgcagc ttggcgatcg cgtccggcgt ccacatgtgc tcctcgcctc
     5641 gcgtgcgcca ggcgtattcg ccgccggcat ccagcatctc ggccagcagc gggtcgtcgc
     5701 cgaaggcggc gcggtgcatg cgcagcgctt cctcggcgat ctcgaacacg cccatgccgc
     5761 ccacctgcga cgccgtgccg cggaagtact tgcccaccac gccggcatcc aggccgatgg
     5821 cctcgaacag ctgcgcgccg cagtacgaca tgtacgtgct gacgcccatc ttggacatga
     5881 tcttcgacag tcccttgccg accgccttga tgtagttgta ggtcgccttc tcggccgaca
     5941 gctggcccgg cagctcggcg tgcagcgacg ccagcgtctc gagcgccagg tacgggtgca
     6001 cggcctccgc gccgtagccg gcgagcacgg caaagtggtg cacctcgcgc gccgagcccg
     6061 tctccacgac caggcccgcg gtcgtgcgca ggccctcgcg caccaggtgc tggtggatgg
     6121 ccgacagcgc cagcagcgcc gggatcgcga cggagtcctt gccgacacgg cggtccgtga
     6181 tgatcaggat gttgtggccg ccgcggatcg cgtccaccgc ttcggcgcac aacgacgcca
     6241 gcttggcctc gacgccctcg cggccccacg agatcggata gacgatgtcg atctcgtgcg
     6301 gcttgaactt gccgaacgtg tgcttctcga tcgcgcgcag gcgggccatg tcgtcgaagt
     6361 cgagcaccgg ctgcgagacc tccaggcgca tctgcgggtt gaccgcgttg atgtccagca
     6421 ggttgggctt ggggccgatg aacgagttca gcgacatcac gatcgcctcg cggatcgggt
     6481 cgatcggcgg gttcgtgacc tgcgcgaaca gctgcttgaa gtagttgtac agcggcttgt
     6541 tcttgtcgga caggacagcc agcggcgagt cgttgcccat cgagccgatg ccttcctcgc
     6601 cgagcgtggc catcgggctg agcaggaact tgatgtcctc ctgcgtgtag ccgaaggcct
     6661 gctggcgatc gagcagcgtg gtggccgcgt cctcgctggc gggcgccgcg agcaccggga
     6721 tctcgtcgag cttgacgcgc acgttgtcga tccactggcg gtacggcttg gccgacgcga
     6781 actggctctt gagctcctcg tcgtcgacga tgcggccctg ctcgatgtcg atcaggaaca
     6841 tcttgcccgg ctgcaggcgc cacttcttga tgatcttgtt ctgcgggatg tcgagcacgc
     6901 ccgactccga cgccatcacg accaggtcgt cgtcggtgac gatgtagcgc gccgggcgca
     6961 ggccattgcg gtccagcgtg gcgccgatct gcttgccgtc ggtgaacacc atcgcggccg
     7021 ggccgtccca cggctccatc atcgcggcgt ggtattcgta gaacgcgcgg cggcgtgggt
     7081 ccatcacggt gtgctgctcc caggcctcgg ggatcatcat catcgcggcg tgggcgagcg
     7141 ggtagccggc catcgtcagc agctcgagcg tgttgtcgaa cgtggcggtg tcggactggc
     7201 cctcgaagct gatcgggtac agcttcttga ggtcctcgcc cagcaccggc gacttcatca
     7261 ccccctcgcg ggcgcgcatc cagttgaagt tgcccttgac ggtgttgatc tcgccgttgt
     7321 gcgccaccat ccggtacggg tgggccagcg gccactcggg gaaggtgttg gtcgagaaac
     7381 gctggtggac gagcgcgagc gcggacacca cgcgcgggtc ggccaggtcc ttgtagtact
     7441 tgccgacctg gtcggccagc aacaggccct tgtagatgat ggtgcggcag ctcatgctgg
     7501 gcacgtagta ctcgcggctg tgggtcagct tgagcgcctg gatggcggcc gaggccgtct
     7561 tgcggatcac gtacagcttg cgttccagcg agtcgggcac gatgacgtcg gcaccgcggc
     7621 cgatgaagat ctggcgcagc accggctcct tggcgcgcac ggtgggcgac atcggcatct
     7681 cgcggtcgac cggcacgtcg cgccagccca gcagcacctg gccttcggcc ttcacggcgc
     7741 gctcgagctc ctgctcgcag gccaggcgcg aggcgtgttc cttgggcagg aagatctggc
     7801 cgacgccgta ttcgccgggc ggcggcagtt ccacgccctg gtcggccatc tcggcgcgga
     7861 agtattcgtc ggggatctgg atcaggatgc ccgcgccgtc gcccatgagc ttgtccgcgc
     7921 cgaccgcgcc acggtggtcg aggttctcca ggatcttcag gccctgcatg acgatcgagt
     7981 gcgccttctg gcccttgatg tgggcgacga agcccacgcc gcaggcgtcc ttctcgttgg
     8041 aagcgtcgta caagccgccc gcggccgcgg aggcgatgtc gatcggcgtg ttggcttggt
     8101 ctcgggcggc ctggctcatg gcgggtcctt ggtgcgctgg aaaacccgaa gactaaagga
     8161 ggtcgccttc ctcggcaaag gcattaaata ggggtcagtg tgattaagtg tgattgctcg
     8221 tgattggtgc gttgtttaat agggccatag tcaattcgat ggttcagagg cccaatacct
     8281 gcaccaagct ggtgatttcc gtcttgccgc ggccggtcga ccggattttg atcgtggcgg
     8341 agtccgtcgc cgcgcagcgc tgcctgcggt cgtcgcggaa tgttttgctc gggtattggg
     8401 gccagcgggg agcgtcgccg ggccgacgga tggcgggttg cgcctgcggc taacccgccc
     8461 tacggtgtct atccggcagg cggccagggc tccgcagggc gggtcagccg aacgcgtgac
     8521 ccgccacggg gacagcgccg cctggattca tggtgccggc gcgacgaccg tgcggcgggg
     8581 ccgaccgcgg ggccgcggca ccgtctcgcg gcccagttgc gccgcgagac ggcgctggcc
     8641 gtccgccccg gcgatgggcc agccgcggcg agtggcctgc gagatcgcct gggcctcgcg
     8701 ctggctgacc ccttcgtcga ggaagccgcg ccaggcgccc tcacgctcga acggcgtgtt
     8761 gccgagcgcc cagtagcgcg catggtcgac gatcagcgga tcgcggcgtc gccccaggtg
     8821 gtgcgcgcag ctcgaccacg cgtagtcggc ggcctcggcg accaggccgc tgcgctgcgc
     8881 atggctgtcg atgtagcgca tgcacgccag cagcgagtca tcgggatcga ccagcgcggc
     8941 ccggaagcgc ccttcccaca gcgtgccggt gcgcccgtgc cgccggttga acgcggcgac
     9001 gtagcgtcgg ccgagcgact gcatcatcgt gctgaggccg tcggcgctgt cgcgcggcgt
     9061 ggccagcagg tgcacgtggt tggacatcag cacgtaggca tggatgtcga cccgcgccac
     9121 gagactggcc tcgcgcaggt gcgcgcacca ggcctggcga tcctcgtcgt cgacgaagat
     9181 gggctgccgg ttgttcccga tctgcatcag gtggtgcggc aggccggaaa gcgagaggcg
     9241 ggtcagacgg gccatgcggg gagtttagct gacccctttt gggccggggt gtccgcgagc
     9301 ggagtcgctc gtgttcagcc cgccggccgc ccgagcgacg cccactcgcc gaacagcacc
     9361 tggcccgtga gccgctggtg ttccttcagc gcgaagcggt ccgtcatgcc ggcgatgtag
     9421 tcggccaccg cgcgcgggcg gtcgacggca tgctggaact cgtcgctcat ctcggcgggc
     9481 cggtccaggt agatcgcgaa cagcgcgcga acgatctcct tggcgcggtc ggtcgtggcg
     9541 ttgaccgcgg gatggcggta caggttggcg aagaggaagc gcttgagctc gctgctctcg
     9601 tggcgcatcg ccgggctgaa gcgcatcagc accggcagct cgcgcacggc ctgtgggctg
     9661 gcgggcgcgg cggcgtcgat cgctgcctgg ctggtggcga tcaggtcgta gatcatctgc
     9721 gacagcatgc ggcgcagcgt ctcgtgcacg gtacggcgcg gcggcaagcc gcggaggcgc
     9781 gggtgcgcgt ccagagcctc ggcgcgcagt cgcgcgtaca gcggcacgtc gtcgagctgc
     9841 gcgggatcga gcagtcccga gcggatgccg tcgtcgacgt cgtggcagtt gtacgcgatc
     9901 tcgtcggcga cgttggtcag ctgcgcctcg aggccgggct gctggccggc gatgaagcgg
     9961 tagcccacgc cgcccggctc gaccgcctcc agcgcgcgcg cctgggccag cgtgatgcgc
    10021 ttgaggatgc cctcgcgcgt ctccagcgtc aggttcaggc cgtcgaactc ggggtagcgc
    10081 agctcgagtt cgtcgacgac gcgcaggctc tgcaggttgt gctcgaagcc gccgtggccg
    10141 tgcatgcaat cgtccagtgc gtcctggccg gcgtggccga acggcgtgtg gccgagatcg
    10201 tgcgccagcg cgatcgcctc gaccaggtcc tcgtccaagc ccatcgtgcg cgcgacggag
    10261 cgtccgagct gcgccacctc cagcgagtgc gtgagccggg tgcggaacag gtcgccctcg
    10321 tggttcagga agacctgggt cttgtagacg aggcggcgga aggccgtcga atggacgatg
    10381 cggtcgcgat cgcgctggaa ttcggtgcgc gtgggcgcgg ccgcctcggg cacgcgccgg
    10441 ccgcgcgagc gcgcgggatc gctggcgtac ggagggcgtt cgacgagcgg gctggcgatc
    10501 acgtcgaacg tcggttgtca ggccgaggcg gtcagcgcgg cgatcacgtc gacgacgacg
    10561 gcctcgggcg cggtgcgcac gctggcgtgg ccctggccat cgagcagcac gaagcggatg
    10621 tccccgccct gcgccttctt gtcgacgcgc atcaggtcca ggtagcgctg cgggctcagg
    10681 ctcgcgggcc cgcgcacggg caggcccgcg cgcgcgacca gcgcctcgat gcggcgcgcg
    10741 aaggccgcct ccaccagccc gaggcgcgcg gacagctcgg cggccatcac catgccgcaa
    10801 cccacgccct cgccatgcag ccactggccg tagcccaggc cggcctcgat cgcgtggccg
    10861 aaggtgtggc cgaagttgag gatctcgcgc aggcccgctt ccttctcgtc ggcgccgacc
    10921 acgtgggcct tgatctcgca gctgcggcgc accgcgtgcg cgagcgcgtc acgcttgcgc
    10981 gcgcgcaggt cggcgacgtg gtgttcgagc cagccgagga actcgatgtc gatgctggcg
    11041 ccgtacttga tgatctccgc caggcccgcc gacatctcgc gatcgggcag cgtgtcgagc
    11101 acgtcgaggt cgcacacgac ctggcgcggc tggtagaacg cgccgatcat gttcttgccc
    11161 agcgggtggt tgacggccgt cttgccgccg accgacgaat cgacctgcgc gagcagcgtc
    11221 gtgggcacct gcacgaacgg gacgccgcgc atgtagcagg cggccgcgaa gcccgtcatg
    11281 tcgccgacga caccgccgcc cagcgcgtac agcacggtgc ggcgatcggc ctcctgtccg
    11341 agcagttcgt cgaacaccag gttcaggtgc gcgagatcct tgtattcctc gccgtcgggc
    11401 agcgccagcg tgcgcacgcg ggcgaagtgg ggctcgagcg ccgcggccag cgccgcgccg
    11461 tacagcgccg cgacggtcac gttcgtgacg atcagcgcga ccgccttgcg attggcctgc
    11521 ggggcccagg tggccgggtc ggacagcaga tcgccgccga tcaggatcgg atagctgcgc
    11581 tcgcccaggt cgatgtggac ctgggtgggc gcggcgcgaa ggagttcgga aggcgtcgac
    11641 atgggacgct attttgcgtc ctcgcgggaa cccgcgctca tgtccagttg catcgagatc
    11701 aggttgacca gcatcgcggc cgacttgtgc gccgtgtcga cgacgatgtc ggccacctcg
    11761 cgatagagcg gatcgcgcac cgtgtagagc tcgcgcagcc gctgtcgcgg atcggccacc
    11821 tgcagcagcg gacgggcggt gtcgcggctg aggcggcgcg cgagatcgtc gacctgggtg
    11881 tgcaggtaga cgacggtggc gcgggccttc atctcggccc ggttggtcgc ccgcagcacg
    11941 ctgccgccac cggtggcgat gacccgccgc tgggaatcgg cgagcgtctc gcggatcacg
    12001 cgctcctcga cttcgcgaaa tgccgcctcg ccttcgcgat cgaagaactc gcggatcgag
    12061 cagccgagat agacctcgat ctcgtggtcg gagtccagaa atggcaactg gcgcatgcgc
    12121 gccagttgtc gcccgatcgt ggattttcca caaccgggca tgcctaccag cgcaatcgcc
    12181 ggcgagcctc gcccttcctg attcaaggct gggcttgaca gagattgcca gaacgactcg
    12241 tggccggatc catgacagtg accgtcgtga ccgccgctgg cgcgacgttc ggcggagcca
    12301 ggttgtaggg gctttcctgg aagggcaggc tgacgttggt ggctccgttg atcaacgatg
    12361 ccggcacgcc gagtcgaccg gacgtggtgg ccttgccttc cgtgccggat acctgcgcgc
    12421 tgaaggtcaa cgtaaagtcg tcccaccagg ccacgttctg gccatattgc agattgaaat
    12481 aggccagtcc gaacgcatcc gtcgtgtcgc tacccgcgct ttgcggcgtg atggcggcat
    12541 ccgcggcgta cggctcgatc ttgccggagc cgttctggtc ttccttctgg ctgttcgagt
    12601 aggtctcgag gacgttgttg cggttgatgt cctcgttgtc gcagacgttc aggttgacgt
    12661 acttccaggc gccggtggaa ccgtcccagt agccgcgata gtagcgcggc aggtcgaccg
    12721 agccggtcac gagcacgccc ttgatcggct ggccaaccga gtcgacgacc tggacggtgt
    12781 acggaaggtt gtagacagcc ttcgtgtcgt cgttcccgat cttcccgttg ttgaagatcg
    12841 agatgctcac gccgctctga gcgaccgtga cgttggcggt cgcggcgttc gggcatgtac
    12901 ccgccgcgaa gtcagtctcg ctccagcagg cgcgcaccgt cagctgctgg ttgccgctgc
    12961 cgcgggtacc tggcgtgtag gtggtctgcg cgacgccgtt ggcgtccgtg tagacgtagc
    13021 ccgatccggc cgccagcgta ccgccgatgc cgctcggatc gccattcagg tcgaagcgca
    13081 cgcgcacgta ctggatcggt gcgttgccgg cacccaggaa caggccgcgg acggtgacga
    13141 aattgctcgt cgagccagcc gtgttgacgt tgaccgtcgc cggattggct gccaccgatg
    13201 ccgacgtcac ggtgcccgtc gccgctgccg tggaggacga cgatgtcgtg accgaggtct
    13261 gcgtcgtcac gcccgctgcc gtcgcactga gcgacaccgt cgccgtcgcc ggcaccgtgt
    13321 agctgtaggt gtagaggccg tttccgtccg tcgtgccgtt ggtggcggtg ccattgagcg
    13381 tgatcacgat cggctcgttg gcgatgctgc cgttgttctt gtcggtcagc tggtactgca
    13441 cggtcgccgt ctggcccgcg gtcgcggcac cggccgatgc cgtcagctgc gcgccgacga
    13501 catccacctt cacggtcttc gtgatggtgc cggacgtgac cacgacatcg atggtgcggt
    13561 tggatttgtc gccgccgatg ccgaggactc cattgaccac gcccgtggcg ctcgtgctgc
    13621 tgccactggt cgtgacgaca ccattgcggt ccgggacgat cgtgaccggc acccctgcga
    13681 cgacattccg gttggcatcg acggccgtcg ccgtgatggt cgccgtctcg gcgcccgtat
    13741 cggcgatgct ggtcttgtcc accgaaacca cgaggtcgga cgccgcggga ggcgcgggcg
    13801 tgggcgacgg cgtcggggca ggcttgttgc cgccaccggc gctgcaaccc gcgatgaccg
    13861 cgccgaccgc caccgcggcg aacagcggct tcaagtattc aataccctgc aatttcatgt
    13921 ttggactctc aatgcgcctg cgtgcggtcg gaaatgatac gcggcgtgat gaagataagc
    13981 agttcggtac gcgtctgcag cttcgacgtg ctgcggaaca ggttgccgag gaacgggatg
    14041 tcgcccagga acggcacctt gtcggtcacg ttgttctcga tctgctggaa caggccgccc
    14101 aggaccaccg tgccgccatt ctcgaccagt gccgtggtct tgatgtgctt ggtgtcgatg
    14161 gcaaagccgg cgctcgtgct gcggcccacg ctgtccttgt tcacttcgac gtccaggatc
    14221 acgttgcctt cgggcgtgat ctgcggggtc acctgcaggc ggatctgcgc cttctggaat
    14281 gccaccgagg tcgcgccgct ggacgtggcc tgctggtagg gcagttcctc gccggcctcg
    14341 accacggcct gcacctggtc ggcggtgatc acgcgtgggc tggagatgat gcggcccgag
    14401 ccgtcttctt cgagggccga cagttcgagg ttcaggaagc ggctggcgct cgagctgaac
    14461 agcgacaggg cgaacgtggc ggccgtcgcg gtggacgaca cgatgccggc ctgcgcgggg
    14521 aagttcacga actgcgagtc ggtgtagggc acggtgccct ggatctggcc cgactggtag
    14581 ccgacggcct cgtaattgcc gcccgggctc acgtactggc tgccgcccag gtggaacgcg
    14641 ttgaacgcgg agccgcccag cttgacgccc agcgcgcggc cgaagttgtc gttggcctcg
    14701 acgatgcggg cctcgatcat cacctggcgt tgcgcgacgt cgatgcgggc gatcagcgcc
    14761 tggatctcct cgagcttgga cgggatgtcg gtgacgaaca gctggttggt gcgcgtctcc
    14821 gcgatcaccg agccgcgctg cgacagcagg cgcgtgccgg cgccgccgga ctggccggta
    14881 ccggtcaggc cgttggccac ttcgagcgcc ttcgtgtagt tcagctggaa cgcctgcatg
    14941 cgcatcggct ccaggtcggc gatcttcttc ttggcctcga ggtcgacctg ctccttggcg
    15001 gccagttcgt ccttcggcgc gatcaacagc acgttgcccg agcgacgctt gtccaggccc
    15061 ttgctctgga gaatgatgtc gagcgcctgg tcccagggca cgtccttcag gcgcagcgtg
    15121 accgtaccgg tgacggagtc gctggtgacg acgttgaagt cgctgaagtc ggcgatgatc
    15181 tgcagcagcg cgcggatctc gatgctctgg aagttcagcg acaggcgctg gccggtgtag
    15241 cccgtgcccg gcacgagctt gttcgggtcg gccttggccg gacgcacttc cagcacgaac
    15301 tggttgtcgg tctggtaggc gctgtgctcc caattgcccg tcggcgtcac gaccatgtgg
    15361 acgtggtcgc cggcctgcgt ggtggtgatc gtctgcaccg gggtgccgaa gtcggtcacg
    15421 tccaggcggc ggcgcaggcg cgtcggcgcg ttggaacgca ggaagtcgac gaccagatcc
    15481 ttgccctgct gcttcaggtc gacgccgacc tggttgctcg ggaggttcac gacgatgcgg
    15541 ccggcgccgt cggtgccgcg gcggaagtcg acgtcgcgca gttcctggac gtcggcgttc
    15601 tggctggcgg cgaagtgcgt gtcggcggcg gccggctgcg cggcggcggc ggcggccgcc
    15661 ggctgcgcca tgtcgagtgc gatcagcagc gacttgccct ggatctcggc gtggtagctc
    15721 gaaggcgtct tcaggttcag cacgagacgc gtgcgttcac cggaactggc caggctcacc
    15781 gatcggatgt tgccctggtt gacctcgacg ctggacttgc cggaggcgtt cgtcacgccg
    15841 ggcaggtcga tcgcgacgcg cggcggcgcc tgcacggcga agcccgccgg cacggccgac
    15901 agcggctcgc tgagctcgac gcgcacgacg tccgtgccgc cttgctgcaa acccgtcacc
    15961 gacgtgatct tgggctccgc ccacgacgtc agcggcaaac ccaacgccaa aaccagcgct
    16021 gctcccgcgg tccaactccg catagtccga ttctcctgac tgctgttcat cgcgccttct
    16081 cctgaagctg gagggtgctg gtgcgctcta tccattcacc cgtggggtct tgcacggttt
    16141 cccgcaaggt aatctgtgtg tccgtgatgg cggtgatctg gccgtagttc tgcccgatcc
    16201 agtcgcccac cttcacgtcg tgcagccggt tctccaccat gagcagcgcg tgcgccttgc
    16261 cgccttgctg gagcgagccg accatcgtca tgctgtccag cgggtacgac tcgagttcct
    16321 gcgtcgcgtg cgccttggca gccgccagca gcgcactgcc cttgccgttg gacggaccgc
    16381 ccacggggat cagcttctgc gtgccgaacg gctcgacgcc ggtgacgccc aggtagggct
    16441 gcgggtcgaa cttcttgggc ggatagatcg gcgcgatgtt cggcttgatt tccttgtgct
    16501 gctgctccat ccactggatc agctcgtcgt tgtcggcggt gcatgcgccg aggccggaca
    16561 acagcagcgc ccccgccgtg atgcagagaa gacggttcat ttcttgtcct tcgcgccagc
    16621 cttgcgggcc tcgacgacct ccgcctggtc gaggtagcgg taggtgcgcg ccacggcttc
    16681 catcgacagg ccgccgccag ccgacttctg gtcggggctg atcgacaggt tgtgcagggt
    16741 cacgatgcgc gagagcgcgg cgatgtcggc cgcgaaggcg ccgatgtcat ggaagcggcc
    16801 ggtgacgcgg atcgcgatcg gcagctcggc gtagtactcc ttggtctcgg tcgcgccagg
    16861 gatgaactgg tcgatgacga ggccgcggcc gacgccggcc tggttgatgt cggtcagcag
    16921 cgcgtccatc tcggccttgc ccggcagctg cttctcgagc tgcgtgacgt aggtctggac
    16981 ctcttccttc tgcttcttca acgcgtccag gttgatcgcc tgcgcgagct tggtcttgta
    17041 ctcggccttg agcgcgggtt ccttgtcgcg cgcagcctgc aggtcgtccg cctgcgagct
    17101 gagttcgacg aaccagccga tgacgacgac ggcgatcgcc acgaccgccc agacggacag
    17161 gcgcggcagc aacggccact ggccgggctg gttggggttc aggtcgcgga actgactcag
    17221 tccgccacgc agcgcggcgc cgatgtccgg gcccttgttc tttgcagtag ccatgcttac
    17281 ctcgtcgccg tcgaagcggc cggagccgtt gcgccagcgg cagccgcgcc ggaagccgca
    17341 cccgcggcgc cggaggcggc agcgcccgga gccggcggac gcttgagcgt cacgctcatg
    17401 gagaaggcga acatccgccg ctggtccttg ctgccggcga cctgcatggt ctgggcacgg
    17461 atctcgatca gctggggttt ctcgagccag ggcgagttgc tggcggtgtt gcgcaggaat
    17521 tcggagatgc gctcgttggt ctgcgccacg ccggtgatct tgacctgctg ggcggcctgc
    17581 ttgacgctcg tcaggaacac gccttcgggc gtctgcttga cgagctcgtt gagcaggtag
    17641 accggcatgt tgcggtcggc ctggagatct tccaccgcct tctggcgcgc cttcagggcc
    17701 tcgatctcgg ccttgagggt cgcgacgtcg gcgatctggg cgtccaggcg cttgatctcg
    17761 ctcgagagga actggttgtg ctcctcctgc gtgctgatct gcgtgttgac gaaggtgtac
    17821 caggccagga cgatgccggc gccgacgagc acggcgaagc cgatgccggc gaagaacgcc
    17881 tgcttgcgtt gctggcgccg cgcttcgcgg tgcggcaaca ggttgatcag aatcactgca
    17941 cgaacctccg catcgccagc ccgcacgccg tcaggtaaga gggcgcctcg cgccgcagct
    18001 tgccttcgcg cacggcgctc cccaacttca tgttctcgaa cggattgacg acccggcagg
    18061 cgaagccggt cacttccttg acccgctcgc gcaggcccga cagcgacgcc gtgcctccgg
    18121 ccagcatcac gtagtcgacc ttgtggtgcg gcgtgctggt gaagaagtac tgcagcgcgc
    18181 ggccgatctc atgggccagg ctgtcgacga acggcatcag cagcgtcgat tcgaaatcct
    18241 cgggcaggtc gccggcgagc ttcttctgct cggcttcctc gaacgagaag ccgtactggc
    18301 gcgagatcag ctgggtcagc tgggcgccgc cgaaggtctg gtcgcggtcg tagagcatct
    18361 cgtcgccgcg cag
//
LOCUS       DATCRO010000901         9674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_471466, whole genome shotgun sequence.
ACCESSION   DATCRO010000901 DATCRO010000000
VERSION     DATCRO010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471466"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1058
                     /locus_tag="VIN75_24535"
     CDS             195..1058
                     /locus_tag="VIN75_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY8977404.1"
                     /translation="MTAARRFARPWLLAALAGLSTAAAAAPDAPPPTESLKPPVGWTL
                     AWSDEFDVDGLPDPKKWGNDTYFNKRGWFNHEQQYYSGPRAENAVVHGGRLLITARRE
                     TPSSQADWGGQKYTSARLLTRGLAEWTYGFFEIRTKLPCGLGTWPAIWMLGTHGDWPA
                     NGELDLMEHIGRNPGRVMSTVHTPAGSGAHGVGASVTVPDACGAFHRYQMLWTDRDIT
                     FGIDGVNNLTYPRLDVGAAGAERAWPFDKPQFLLLNIAIGGDLGGPVDDGIFPATMEV
                     DYVRVWQPPKR"
     gene            complement(1175..1702)
                     /locus_tag="VIN75_24540"
     CDS             complement(1175..1702)
                     /locus_tag="VIN75_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8977405.1"
                     /translation="MRTLRRTTLLLALPLAAGLAQAQAAPAASAPAERRNWFDDPFVA
                     ATQGLPGCPVPDAPLLTEAQMQHETHWRAERGTSCFRSGRCRLPNAYLYDKEIAPRVV
                     KAIAAAGSFDNTSIWVEGQRRWVWLKGCVSTPEQAAELERMVRLIDDVEAVIPQLIVG
                     TTMPPGTPVYRLPPQ"
     gene            1823..2338
                     /locus_tag="VIN75_24545"
     CDS             1823..2338
                     /locus_tag="VIN75_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY8977406.1"
                     /translation="MSLSMYTATVPPFRAMLKNLSAVMAKAEAHCEARKIDPAAFLAS
                     RLYPDMLPFTAQVQIASDNVKGAAGRLSGAEIPKFEDNEKTFAELQARIDKTIAFLDT
                     LDESQFDGAAERDIVLQLRDRKLEFKGQDYLFQWAIPNVYFHITTAYALLRHGGVEIG
                     KKDYLSGGAAK"
     gene            2560..2991
                     /locus_tag="VIN75_24550"
     CDS             2560..2991
                     /locus_tag="VIN75_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8977407.1"
                     /translation="MRQFIRHPLDVPVEIRRDRCGPATAFQTQDISLGGLAVNSDEPV
                     STGALIEVRIAYVDPVFEARARVAWCRARRDGAGYEVGVSFLDPEDVFRARMVAQVCF
                     IEDYRRSIARAEGRELSSEEAAGEWIAKYAHKFPGASGGLQ"
     gene            3096..4001
                     /locus_tag="VIN75_24555"
     CDS             3096..4001
                     /locus_tag="VIN75_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY8977408.1"
                     /translation="MKWEGQRQSDNVEDLRDQDGGGGGGPGFGGFGVRHIGIGTIVIA
                     LVGGWIFGINPLTILGLLSGGGAPVQQTAPQQVPGPRPAHTPQDDRMKAFVATVLADT
                     EDVWTQVFKERGGTYRDPKLVLFRNATPTACGRGESATGPFYCPGDEKVYIDLSFYDL
                     LRTKLGAPGDFAQAYVIAHEVGHHVQKLLGISEKVDAARRQSSEARSNALSVRLELQA
                     DCFAGIWANRAQATKQIIEAGDIDEALNAATQIGDDTLQRKASGTVQPETFTHGTSAQ
                     RVHWFKQGFETGQIEQCDTFKPGSP"
     gene            complement(4018..5130)
                     /locus_tag="VIN75_24560"
     CDS             complement(4018..5130)
                     /locus_tag="VIN75_24560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8977409.1"
                     /translation="MVQNSDIVYSATQHIDRLMRRVVLLLCGAGVLSVLAIWHMEVVD
                     GTLEPFNRFGYPAMVLVFSVGFVALWRFPAVLPWVRWAGFFSICGVLMTDLWGQTHES
                     GPLLDNYNALTLWNWLPLCYTMAFFMLQSRHAFIVAAAMLAFFAWCTILRSTAGTSYA
                     LQDRSLMINTLISHAVLVSCLSGLAWLKHVVSVQGEQASRLNRLAATDPLTGLANRRQ
                     TLELLEQLARDGRLERSPVLMLCDLDHFKNINDGWGHAMGDEVLVAVAGALRASTRDS
                     DTVSRWGGEEFLIVLPDTRGADPAELAERLRTRVESLQVADRHQCPVPVTLSIGIARL
                     AAGEAGASWLKRSDEALYRAKAEGRNCCRMAEPPEA"
     gene            5373..7262
                     /locus_tag="VIN75_24565"
     CDS             5373..7262
                     /locus_tag="VIN75_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8977410.1"
                     /translation="MLGYFERLVDPWPDVEPPPPPKGVFPFIWACSAGVRRFVLGMTL
                     LTAIIGAFEALLFAMLGHVVDWLAHVEPAKLWAQEGGTLVALGVILTLSPLLVAVQAS
                     LKYQTLFGNFPMRLRWNFHRMMLAQSMSFYQDEFAGRVAAKVMQTALGVRDVVTTMTD
                     ILVFVIIYFVTMIAVVGSFDLWLLGPFVGWLLLYGIALRYFVPRLGKVGALQADARAV
                     MTGRITDAYTNISTVKLFSHARREASYARQAMHEFIVTAYQQGRYITGIEIVNHVLSM
                     GLIMSTTGVTLWLWTRGQVGAGAVAAATAMALRLNGISHWIMWELASLFENIGTVTDG
                     MNTLSRPHAIVDAPGAPELVVPHGEVKFDDVGFAYGGKRQVIDRFSLTIKPGEKIGLV
                     GRSGAGKSTLVNLLLRFYDVESGRLLIDGQDVSKVTQNSLRAHVGMVTQDTSLLHRSV
                     RDNILYGRPDATDAEMIAAAERAEAGEFIQSLADAKGRKGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYDLMEGKTVIAIAHRLSTIAAM
                     DRLVVLDKGRIVEEGDHRSLLARGGLYARLWAHQSGGFLGEETDPEAPPERADALVTE
                     DDDAE"
     gene            complement(7276..8652)
                     /locus_tag="VIN75_24570"
     CDS             complement(7276..8652)
                     /locus_tag="VIN75_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977411.1"
                     /translation="MPTESARAPSFPLVVLATSMAFVVGQLDVTIVNVALPAMGRALG
                     ASVAGLQWVVDAYAVAFAAFMLSAGALGDRFGARRAYQSGMAVFALASIGCALAPAAA
                     WLDAARALQGLGAAIMLPNSLALLNHALAHDARARARGIGWWTAAGAVAIAAGPVLGG
                     VLVATVGWPAIFWANAPLCAAGLWLVRRLRETPIGRDRPPLDVAGQVLATSLLAALIA
                     TLIESRTRGAGDVLVVAGAVATIVLAVSLALVERRAAAPVIATDLFALRDFRNAVFLG
                     TVVNATYYGAIFVIALFLQGARHFAPMQAGLAFLPLTAGFFLSNVASGWVIGRLGTRK
                     PMIAGTLIDLAGFGVLAWASTQDSLVPMLAGFLLIPTGMGLAVPAMTTTVLSAVEKDR
                     SSLAAAILNTARQAAGAIGVAAFGALAHGDAGHVAAGLRITCGVAMLLLAGAALAAAR
                     LRGVPPHD"
     gene            8936..9598
                     /locus_tag="VIN75_24575"
     CDS             8936..9598
                     /locus_tag="VIN75_24575"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8977412.1"
                     /translation="MSGSPQTVIAPMPAASNKSARSAAASTTRAAGAKPARNAGDTRA
                     RILAAARVRFSREAYENVGTRDIAADAGVDAALVNRYFGSKEKLFDEAIEDAFAIKDH
                     FDALDMSRFGETITTMVIEGSAERRAAGFDALGILLRSSGSPATHVRVSERFHADFVL
                     PLARLLRGRDAELRAALIASHLIGLATMRHALASPLLLADAARRKTIASVGEAIQDCV
                     DG"
BASE COUNT         1501 a         3471 c         3394 g         1308 t
ORIGIN      
        1 gtggacggca tcgccgagct gctgcacgcc aaggtcgacg gcagccatgc ggggctcgcc
       61 gcgggcgcca gcgtcggcct gcagcccgtc acggaatcgg tcctggcgtt cgacgacgcc
      121 ggccggcgcg tcgcatgagc gtccgcacgg ccgctccgaa gggcgctcgc gccgcgcaag
      181 gcggagattg cccgatgacc gccgcacgtc gcttcgcgcg tccctggctg ctggccgcgc
      241 tggccggcct gtcgacagcc gccgcggccg cgcccgacgc gccgccgccg accgagtcgc
      301 tgaagccgcc cgtgggctgg acgctcgcct ggagcgacga attcgacgtc gacggcctgc
      361 ccgacccgaa gaagtggggc aacgacacct acttcaacaa gcgcggctgg ttcaaccacg
      421 agcagcagta ttactcgggc ccgcgcgccg agaacgccgt cgtgcacggc ggccgcctgc
      481 tgatcaccgc gcgcagggag acgccgtcgt cgcaggccga ctggggcggc cagaagtaca
      541 cgtcggcgcg cctgctcacg cgcggcctgg cggagtggac ctacggcttc ttcgagatcc
      601 gcaccaagct gccgtgcggc ctgggcacct ggcccgcgat ctggatgctg ggcacccacg
      661 gcgactggcc cgccaacggc gagctggacc tcatggagca catcggccgc aacccgggcc
      721 gcgtgatgag cacggtgcac acgccggccg gcagcggcgc gcacggcgtc ggcgcatcgg
      781 tgacggtgcc cgatgcctgc ggcgcgttcc accgctacca gatgctgtgg accgaccgcg
      841 acatcacctt cggcatcgac ggcgtcaaca acctcacgta cccgcggctg gacgtcggcg
      901 ccgccggcgc ggagcgcgcc tggccgttcg acaagccgca gttcctgctg ctgaacatcg
      961 ccatcggcgg cgacctcggc gggccggtgg acgacggcat cttcccggcg acgatggaag
     1021 tggactacgt gcgcgtctgg cagccgccga agcggtaggg cgggtcgcga cccgccgttc
     1081 ggaggggtca ggccttcgcc tcgccctcgc gagcggcgtc gcggattccg accagtgccc
     1141 ggcgcaaggc cgaggaaagg ccagacccct ccggtcactg gggcggcagg cggtagacgg
     1201 gcgtgcccgg aggcatcgtc gtcccgacga tcagctgggg gatcacggcc tcgacgtcgt
     1261 cgatcaggcg caccatgcgc tccagttcgg cggcctgctc gggcgtcgag acgcagccct
     1321 tgagccacac ccagcgccgc tggccctcga cccagatcga ggtgttgtcg aagctgcccg
     1381 cggcggcgat ggccttgacc acgcgcggcg cgatctcctt gtcgtacagg taggcgttgg
     1441 gcaggcggca gcggcccgag cggaagcaac tggtgccgcg ttcggcgcgc cagtgcgtct
     1501 cgtgctgcat ctgggcctcg gtcagcagcg gggcgtccgg caccgggcag ccgggcaggc
     1561 cctgcgtggc ggcgacgaac gggtcgtcga accagttgcg ccgctccgcc ggcgcggacg
     1621 ccgcgggcgc cgcctgcgcc tgcgcgaggc ccgcggccag cggcagggcc aggagcaggg
     1681 tggttcgacg gagggttcgc atggggccat cttagagcgc ccgggcccgg tcgtacccgt
     1741 gcaagacctg accccggcaa ggcctgtcgc gctgcgctct aatggcccgc gttcccgtcc
     1801 aacccgtttt ccggagaccg tcatgtccct gtccatgtac accgccacgg tgccgccctt
     1861 ccgcgccatg ctcaagaacc tgtccgccgt catggccaag gccgaggcgc actgcgaggc
     1921 caggaagatc gacccggccg cgttcctcgc gtcgcggctg tatccggaca tgctgccgtt
     1981 cacggcgcag gtccagatcg cgtccgacaa cgtcaaggga gcggccggcc gcctgtcggg
     2041 cgccgagatc ccgaagttcg aggacaacga gaagacgttc gccgagctgc aggcccgcat
     2101 cgacaagacc atcgccttcc tcgacacgct cgacgagtcg cagttcgacg gcgccgccga
     2161 gcgcgacatc gtcctgcaac tgcgcgaccg caagctcgag ttcaagggcc aggactacct
     2221 gttccagtgg gccatcccga acgtctactt ccacatcacg accgcgtacg cgctgttgcg
     2281 tcacggcggc gtcgagatcg gcaagaagga ctacctcagc ggcggcgccg cgaagtaagc
     2341 acttcgccgg cgcaccgcaa cccggggccc cgcgggggtc ccgcgcgctg acggcgtgcc
     2401 cggcaatggc cggatctggc ccgcatccca cgttcgaatc cgcccatcgg gcggcctgcg
     2461 cctgtacaac cattggcatg gcggggcttc gccccgggtt gtacaaagcg acgagcagcc
     2521 tgtgcaggac ggcagtcgcg aaccgcagga agacgaggca tgaggcagtt catccggcat
     2581 ccgctcgacg tcccggtcga gatccgacgg gatcggtgcg gtcccgccac ggcgttccag
     2641 acccaggaca tcagcctggg cggcctggcc gtgaactccg acgagccggt gtcgaccggc
     2701 gcgctgatcg aggtgcgcat cgcctacgtc gaccccgtct tcgaggcgcg cgcccgcgtc
     2761 gcctggtgcc gggcacggcg cgacggcgcc ggctacgagg tgggcgtcag cttcctcgat
     2821 cccgaggacg tgttccgcgc ccgcatggtg gcgcaggtct gcttcatcga ggactaccgg
     2881 cgctcgatcg ctcgcgccga gggcagggaa ctcagctccg aggaggcggc tggcgaatgg
     2941 atcgccaagt acgcgcacaa gtttcccggg gccagcggcg ggcttcaata gtaggcaacg
     3001 cggccatccg gcccgacaat tgcccgatgg tgtcccgctg agccaggtcc ccgacgcccc
     3061 cgtcgcgggc tggcccgacg ccggaggagc atgcgatgaa atgggaaggc cagcgccaga
     3121 gcgacaacgt cgaggacctg cgtgaccagg acggcggcgg aggcggcggc ccgggcttcg
     3181 gcggcttcgg ggtgcgccac atcggcatcg gcaccatcgt catcgcgctg gtcggcggct
     3241 ggatcttcgg catcaacccg ctcaccatcc tcgggctgct cagcggcggc ggggcgccgg
     3301 tgcagcagac cgcgccgcag caggtgccgg gcccgcgccc ggcccacacg ccgcaggacg
     3361 accgcatgaa ggccttcgtc gccaccgtgc tggccgacac cgaggacgtc tggacgcagg
     3421 tgttcaagga gcgcggcgga acctaccgcg accccaagct ggtgctgttc cgcaacgcca
     3481 cgcccaccgc ctgcggccgc ggcgaatcgg ccaccgggcc gttctactgc ccgggcgacg
     3541 agaaggtcta catcgacctg agcttctacg acctgctgcg caccaagctc ggggcgccgg
     3601 gcgacttcgc gcaggcctac gtgatcgcgc acgaggtggg ccaccacgtg cagaagctgc
     3661 tgggcatctc ggagaaggtg gacgcggcgc gccgccagtc cagcgaagcg cgctcgaacg
     3721 cgttgtcggt gcgcctggag ctgcaggccg actgcttcgc cggcatctgg gccaaccggg
     3781 cgcaagccac caagcagatc atcgaggccg gcgacatcga cgaggcgctc aacgccgcca
     3841 cgcagatcgg cgacgacacg ctgcagcgca aggccagcgg cacggtgcag cccgagacct
     3901 tcacgcacgg caccagcgcg cagcgcgtgc actggttcaa gcagggcttc gagacgggcc
     3961 agatcgagca atgcgacacc ttcaagcctg ggtcgccctg atgatccttg aaccggctca
     4021 ggcctcgggc ggctctgcca tccggcagca gttgcgcccc tccgccttgg cccgatagag
     4081 cgcctcgtcc gaccgcttga gccagctggc ccccgcctcg ccggccgcca ggcgtgcgat
     4141 gccgatgctc agcgtcaccg gcaccgggca ttggtggcga tcggccacct gcagcgattc
     4201 cacccgcgtg cgcagccgct cagcgagctc ggcggggtcg gccccgcggg tgtcgggcag
     4261 cacgatcagg aactcctcgc cgccccagcg cgagacggtg tcggagtcgc gcgtgctcgc
     4321 gcgcagcgcg cccgcgacgg ccaccagcac ctcgtcgccc atcgcgtggc cccagccgtc
     4381 attgatgttc ttgaagtggt cgaggtcgca cagcatcagc acgggcgagc gctcgagccg
     4441 gccgtcgcgt gcgagctgtt ccagcagttc cagcgtctgg cgccggttgg ccaggccggt
     4501 gagcgggtcg gtggcggcca gccggttcag gcgcgacgcc tgctcgccct gcacggacac
     4561 gacgtgcttg agccatgcca ggcccgacag gcacgacacc agcaccgcgt gcgagatcaa
     4621 cgtgttgatc atcagcgagc ggtcctgcaa cgcatacgac gtgccggcgg tgctgcgcag
     4681 gatcgtgcac caggcgaaga acgccagcat ggcggccgcc acgatgaacg cgtggcgcga
     4741 ctgcagcatg aagaaggcca tcgtgtagca cagcggcagc cagttccaca gcgtcagcgc
     4801 gttgtagttg tccagcagcg ggccggactc gtgcgtctgc ccccacaggt cggtcatcaa
     4861 cacgccgcag atgctgaaga agcccgccca gcgcacccag ggcagcacgg cgggaaaccg
     4921 ccacagggcg acgaagccca cggagaacac cagcaccatg gccggatagc cgaagcggtt
     4981 gaacggctcg agcgtgccgt ccacgacctc catgtgccag atcgccagca ccgacagaac
     5041 gcccgcgccg caaaggagca gcaccacccg ccgcatcagg cggtcgatgt gctgcgtcgc
     5101 gctgtagacg atgtcgctgt tctggaccat ggcccttcag gtctggctgg cggctcgttt
     5161 ccctcgtgca ggccgccggt ttatggcgtt cgaccccatt ctgcgcgcat cgcgggcgca
     5221 tccggggcct tcgaatgatg ggggtggggc cggcgccgcg cgccgccggg cacgcgccgc
     5281 gctgcccatt gcgtcacgcg ctcgtccgtt ttcgcgtagc cttgcggctt tcgccccgca
     5341 ctcgcgcggg cgccagactc aggagccccg gtttgctcgg ttacttcgaa agactcgtcg
     5401 atccctggcc ggacgtcgag ccgccgccgc cgcccaaggg cgtcttcccc ttcatctggg
     5461 cctgttccgc cggcgtgcgc cgcttcgtgc tgggcatgac cctgctgacc gccatcatcg
     5521 gcgcgttcga ggcgctgctg ttcgcgatgc tggggcacgt ggtggactgg ctggcgcacg
     5581 tcgagcccgc gaagctgtgg gcgcaggaag gcggcacgct cgtcgccctc ggcgtgatcc
     5641 tgaccttgag cccgctgctg gtggccgtgc aggcctcgct gaagtaccag acgctgttcg
     5701 gcaacttccc gatgcgcctg cgctggaact tccaccgcat gatgctcgcg cagagcatga
     5761 gcttctacca ggacgagttc gccggtcgcg tcgccgccaa ggtgatgcag acggcgctgg
     5821 gcgtgcgcga cgtcgtcacc acgatgaccg acatcctggt cttcgtcatc atctacttcg
     5881 tgacgatgat cgcggtggtt ggcagcttcg acctgtggct gctggggccg ttcgtcggct
     5941 ggctgctgct gtacggcatc gcgctgcgct acttcgtgcc gcggctgggc aaggtcggcg
     6001 cgctgcaggc cgacgctcgc gccgtgatga cgggccgcat caccgacgcg tacaccaaca
     6061 tctccacggt caagctgttc tcgcacgcgc gccgcgaggc gagctatgcg cgccaggcga
     6121 tgcacgagtt catcgtcacc gcgtaccagc agggccgcta catcaccggc atcgagatcg
     6181 tgaaccacgt gctgagcatg gggctcatca tgagcaccac cggcgtcacg ctgtggctgt
     6241 ggacgcgcgg ccaggtcggc gccggcgcgg tggcggcggc caccgcgatg gcgctgcgcc
     6301 tgaacggcat ctcgcactgg atcatgtggg agctcgcgtc gctgttcgag aacatcggca
     6361 ccgtcaccga cggcatgaac acgctgtcgc ggccgcacgc gatcgtcgat gcgccgggcg
     6421 cgccggagct cgtggtgccg cacggcgagg tcaagttcga cgacgtgggc ttcgcgtacg
     6481 gcggcaagcg ccaggtcatc gaccggttct cgctgaccat caagccgggc gagaagatcg
     6541 gcctggtcgg gcgttcgggc gcgggcaagt ccacgctcgt gaacctgctg ctgcgcttct
     6601 acgacgtcga gtcaggccgg ctgctgatcg acgggcagga cgtctccaag gtcacgcaga
     6661 acagcctgcg cgcgcacgtg ggcatggtca cgcaggacac ctcgctgctg caccgttcgg
     6721 tgcgcgacaa catcctgtac ggccgccccg acgccaccga cgccgagatg atcgccgccg
     6781 ccgagcgcgc cgaggccggc gagttcatcc agtcgctggc cgacgccaag ggccgcaagg
     6841 gctacgacgc ccacgtcggc gagcgcggcg tgaagctctc gggcggccag cgccagcgca
     6901 tcgcgatcgc gcgcgtgatg ctcaaggacg cccccatcct gctgctcgac gaagccacca
     6961 gcgcgctcga ctccgaggtc gaggccgcga tccaggcgag cctgtacgac ctgatggagg
     7021 gcaagacggt catcgcgatc gcgcatcggc tgtccaccat cgcggcgatg gaccgcctgg
     7081 tcgtgctcga caagggccgc atcgtcgagg agggcgacca ccgctcgctg ctcgcgcgcg
     7141 gtggcctgta cgcgcggctg tgggcgcacc agagcggcgg cttcctcggc gaggagaccg
     7201 accccgaggc cccgcccgaa cgcgcggacg cgctggtcac cgaggacgac gacgccgagt
     7261 gatcgtgctg cgccgtcagt cgtggggcgg cacgccgcgc agccgggcgg ccgccagcgc
     7321 ggcgccggcc agcagcagca tcgccacgcc gcaggtgatg cgcaggccgg cggccacatg
     7381 gccggcgtcg ccgtgcgcga gcgccccgaa ggcggccacg ccgatcgcgc cggccgcctg
     7441 gcgcgcagtg ttgaggatgg cggcggccag cgacgaacgg tccttctcga ccgccgacag
     7501 caccgtcgtc gtcatcgccg gcaccgccag gcccatgccg gtggggatga gcaggaagcc
     7561 ggccagcatg ggcacgaggc tgtcctgcgt cgatgcccag gccaggacgc cgaagcccgc
     7621 gaggtcgatc agcgtgccgg cgatcatcgg cttgcgcgtg cccaagcggc cgatcaccca
     7681 gccgctcgcc acgttggaca ggaagaagcc ggccgtcagc ggcaggaagg ccaggccggc
     7741 ctgcatcggc gcgaagtggc gcgcaccctg caggaacagc gcgatgacga agatcgcccc
     7801 gtagtacgtg gcgttgacga ccgtgccgag gaacacggcg ttgcggaagt cgcgcagcgc
     7861 gaacaggtcg gtcgcgatga ccggcgcggc ggcgcggcgc tccaccagcg cgagcgagac
     7921 ggccagcacg atggtggcga cggcaccggc caccaccagc acatcgccgg cgccgcgcgt
     7981 gcgcgactcg atcagcgtgg cgatcagcgc ggccagcaac gacgtcgcca gcacctggcc
     8041 cgccacgtcg agcggcggac ggtcgcggcc gatgggcgtc tcgcgcaggc gccgcaccaa
     8101 ccagagtccg gcggcgcaca gcggcgcgtt ggcccagaag atggccggcc agcccacggt
     8161 ggcgacgagc acgccgccga gcacgggccc cgcggcgatg gccaccgcgc cggcggctgt
     8221 ccaccagccg atgccgcggg cccgcgccct ggcgtcgtgc gccagcgcgt ggttcagcag
     8281 cgccagcgaa ttgggcagca tgatggccgc ccccagcccc tgcagcgcgc gggccgcgtc
     8341 cagccaggcc gcggcgggtg cgagggcaca gccgatcgag gccagcgcaa agacggccat
     8401 gcccgactgg tacgcgcggc gcgcgccgaa gcggtcgcca agcgcaccgg ccgacagcat
     8461 gaacgccgcg aacgcgacgg cgtaggcgtc gaccacccac tgcaggcccg ccacgctggc
     8521 gccgagcgcg cgacccatcg cgggcagcgc gacgttgacg atcgtgacgt cgagctggcc
     8581 gaccacgaag gccatcgagg tggcgagcac gacgagggga aaggacgggg cgcgcgcgga
     8641 ctcggtgggc atgggctcat gctagtgtcg tgattgagaa gttggttgaa gaatcccctc
     8701 gacaacgcct gtggccgtcg ttgccgtgct cgcgaggcat cgagcctcgc tccgcgcgcc
     8761 gcctagtcca cagacgtttg cgagaggatt cggcaacgtt tcctctgcgc tcgcgaccaa
     8821 ttcaacgaac ttcccaatca cgacgctagc ggcgtgcaag cgtcgacgct tcgcccaggg
     8881 acgaaggata gcccccgcgg tgggcgcgtt gcgaatgtca acggctgctg acaaaatgtc
     8941 cggatccccg cagaccgtga tcgccccgat gcctgccgcc tcgaacaagt ccgcccgatc
     9001 cgccgccgcg tccaccaccc gcgcggccgg ggccaagccc gcccgcaacg ccggcgatac
     9061 ccgggcccgc atcctggccg cggcgcgcgt gcgcttctcg cgcgaggcct acgagaacgt
     9121 cggcacgcgc gacatcgccg ccgacgccgg ggtcgacgcc gcgctcgtca accgctactt
     9181 cggcagcaag gagaagctgt tcgacgaggc catcgaggac gcgttcgcca tcaaggacca
     9241 cttcgacgcg ctggacatga gccggttcgg cgagaccatc accacgatgg tgatcgaggg
     9301 cagcgccgaa cgccgcgcgg ccggcttcga cgcgctgggc atcctgctgc gctcgtcggg
     9361 cagcccggcc acgcacgtgc gcgtctcgga gcgcttccat gccgacttcg tgctgccgct
     9421 cgcgcgcctg ctgcgcgggc gcgacgccga gctgcgcgcc gcgctgatcg cctcgcacct
     9481 gatcggcctg gcgacgatgc gccacgcgct cgcgtcgccg ctgctgctgg ccgacgcggc
     9541 gcgtcgcaag acgatcgcca gcgtcggcga ggcgatccag gactgcgtgg acggctagga
     9601 gtctgcgcgg cagaaggcgc ctgctgcgaa cggcgataag cggggccttt tcagcggcgg
     9661 ctcctctcgc gtag
//
LOCUS       DATCRO010000902         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_853880, whole genome shotgun sequence.
ACCESSION   DATCRO010000902 DATCRO010000000
VERSION     DATCRO010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853880"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..924
                     /locus_tag="VIN75_24580"
     CDS             130..924
                     /locus_tag="VIN75_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8977413.1"
                     /translation="MNDTTPPLVEFRGLHKAFGGVHAVSDVDLALDAGEVVAVLGHNG
                     AGKSTLMKMLAGAYPIDRGEIRVAGELAHIANPADSQRLGIETIHQTLALADNLDAVA
                     NLFLGRELMTRWNTMDDYAMEAAARDVFAQLNPNFRNIREPVRSLSGGQRQVVAISRA
                     LHFKARILIMDEPCAALGPEETRMVHDLVRRLKARGVGVFLISHDMPDVFALSDRLAV
                     MKNGRVIGTYRTADLTEDEVLGMIIGGKPVTRVAQQHRMLTAEAGS"
     gene            986..2470
                     /gene="xylB"
                     /locus_tag="VIN75_24585"
     CDS             986..2470
                     /gene="xylB"
                     /locus_tag="VIN75_24585"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY8977414.1"
                     /translation="MNIDGTTLGIDLGTSELKLALVDAQGALMASAGAPLTVSRPHPH
                     WSEQAPVDWWNALLAAARTLRASHPAEWAGVRAVGLSGQMHGAVLMDAGGEVLRPAIL
                     WNDTRSAAQCAELVRRLPSLPLIAGNLAMPGFTAPKLLWVAQHEPEVFGRVDRVLLPK
                     DWLRWKLTAAAFSDMSDAAGTLWLDVGRRAWSPQLLAASDMTARQMPALVEGSDVAGR
                     LTPDAALALGLPEGLPVAGGGGDNAASAVGIGAVQSGDGFLSLGTSGVLFVVTDRYRP
                     NPASAVHAFCHALPGRWHQMSVMLSAASCLQWVTRLTGSASEAALLERVEALTDAERD
                     AAPLFLPYLSGERTPHDDPHACGVFFGMDNATDAARLGHAVIEGVAFGLADGLQALRD
                     AGTEVGRLALVGGGARSAHWAQLLADVLDIELQTVAGAEAGGAMGAGRLAWLALGHDE
                     REVCRPPAPRALFQPRAERRAALQRRLERFRALYRHTRDLMPRD"
     gene            2494..>3621
                     /gene="xylA"
                     /locus_tag="VIN75_24590"
     CDS             2494..>3621
                     /gene="xylA"
                     /locus_tag="VIN75_24590"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8977415.1"
                     /translation="MTEYFAGIDKIRYAGPQSREPLSFKWYDKDRIVLGKRMEDQLRF
                     AVCYWHSFCWNGFDPFGYDGTFERPWHRVADPMAAARAKLDVPYYCFHDRDVAPEGAT
                     PRESHDNFLRLVDVLGEKQQATGRKLLWGTANLFSHRRFMSGAASNPDPEIFKLAALQ
                     VRDAMDATLKLGGENYVLWGGREGYETLLNTRLGQELDQLGRFLNMVVEHKHKIGFKG
                     AILIEPKPREPSKHQYDFDTATVYGFLVRYGLEKEVKVNIEANHATLSGHSFEHEIAT
                     AGALGILGSLDMNRGDPQLGWDTDQFPNNVPETAMALYHVIQAGGLGSGGLNFDAKVR
                     RQSIDAADLFHGHIGGMDVCAQALLVAEKMINDGRLKAAVDS"
BASE COUNT          543 a         1347 c         1252 g          479 t
ORIGIN      
        1 catgaacgcc ccgtccggcc gctccgaagg gcgctcgcgc cgcagcccgc agggcggagg
       61 tcgccttgtg agcccgcacg gccgcccgaa gggcgaatcg accgcagtcc gcgggacgga
      121 ggtcgcccta tgaacgacac cacgccaccc ctggtcgagt tccgcggcct gcacaaggcc
      181 ttcggcggcg tgcacgccgt cagcgacgtc gacctcgcgc tcgacgccgg cgaggtggtc
      241 gccgtgctgg gccacaacgg cgcgggcaag tccacgctga tgaagatgct cgcgggcgcc
      301 tacccgatcg accgcggcga gatccgcgtc gccggcgagc tggcccacat cgccaacccg
      361 gccgactcgc aacgcctggg catcgagacg atccaccaga cgctcgcgct ggccgacaac
      421 ctggacgccg tcgccaacct gttcctcggc cgcgaattga tgacgcgctg gaacacgatg
      481 gacgactacg cgatggaggc ggccgcgcgc gacgtcttcg cgcagctgaa cccgaacttc
      541 cgcaacatcc gcgagccggt gcgctcgctg tcgggcggcc agcgccaggt ggtcgcgatc
      601 tcgcgcgcgc tgcacttcaa ggcgcgcatc ctgatcatgg acgagccctg cgccgcgctc
      661 ggtcccgagg agacgcggat ggtgcacgac ctcgtgcgcc ggctcaaggc gcgcggcgtc
      721 ggcgtgttcc tgatctcgca cgacatgccc gacgtgttcg cgctgtccga ccggctggcc
      781 gtgatgaaga acggccgcgt gatcggcacc taccgcaccg ccgatctcac cgaggacgag
      841 gtgctcggca tgatcatcgg cggcaagccg gtgacgcgcg tggcgcagca gcaccggatg
      901 ctcaccgcgg aggccggatc atgagcgcgc gcatggccgc gccgaagcgc gctcgcaccg
      961 cagcccgcag ggcggaggtt tgctcatgaa catcgacggc acgacgctgg gcatcgacct
     1021 gggcacctcc gaactcaagc tcgcgctggt cgacgcgcag ggcgcgctca tggcgagcgc
     1081 cggcgcgccg ctgacggtgt cgcgcccgca tccgcactgg tcggagcagg cgccggtcga
     1141 ctggtggaac gcgctgctcg cggccgcgcg cacgctgcgc gcgagccatc ccgccgaatg
     1201 ggccggcgtg cgcgcggtcg gcctgtccgg gcagatgcac ggcgccgtgc tgatggacgc
     1261 gggcggcgag gtgctgcggc ccgccatcct ctggaacgac acgcgcagtg ccgcgcagtg
     1321 cgcggagctc gtgcgccgcc tgccgtcgct gcccttgatc gccggcaacc tcgcgatgcc
     1381 gggcttcacg gcgcccaagc tgctgtgggt ggcgcagcac gagcccgaag tgttcggccg
     1441 cgtcgaccgc gtgctgctgc ccaaggactg gctgcgctgg aagctgacgg ccgcggcgtt
     1501 cagcgacatg tccgacgccg cgggcacgct gtggctggac gtcggccgcc gcgcgtggtc
     1561 gccacagctg cttgccgcca gcgacatgac ggccaggcag atgccggcgc tggtcgaagg
     1621 ctcggacgtc gccggccgcc tgacgcccga cgccgcgctc gcgctgggcc tgcccgaggg
     1681 cctgccggtg gccggcggcg gcggcgacaa cgcggccagc gcggtgggca tcggcgccgt
     1741 gcagtcgggc gacggcttcc tgtcgctggg caccagcggc gtgctgttcg tcgtcaccga
     1801 ccgctaccgc cccaacccgg cgtccgccgt gcatgcgttc tgccacgccc tgcccggacg
     1861 ctggcaccag atgagcgtga tgctgtcggc cgccagctgc ctgcagtggg tgacgcggct
     1921 caccgggagc gcaagcgaag ccgcgctgct ggagcgcgtc gaggcgctga ccgacgccga
     1981 gcgcgacgcc gccccgctgt tcctgcccta cctgtcgggc gagcgcacgc cgcacgacga
     2041 cccgcacgcc tgcggcgtgt tcttcggcat ggacaacgcg accgatgccg cgcgcctggg
     2101 ccacgccgtc atcgagggcg tcgcgttcgg cctggccgac ggcctgcagg ccttgcgcga
     2161 cgccggcacc gaggtcggcc gcctcgcgct ggtcggtggc ggcgcccgca gcgcacactg
     2221 ggcccagttg ctcgccgacg tgctcgacat cgagctgcag acggtcgccg gcgccgaggc
     2281 cggcggcgcg atgggcgcgg gccggctcgc ctggctcgcg ctcggccacg acgagcgcga
     2341 ggtctgccgc ccgcccgcgc cgcgcgccct cttccagccg cgcgccgaac gccgcgccgc
     2401 gctgcagcgc cgcctcgaac gcttccgcgc gctctatcgc catacccgcg acctgatgcc
     2461 gcgcgactga cccttccctc caggagaccc accatgaccg aatacttcgc cggaatcgac
     2521 aagatccggt acgccggccc gcaatcgcgc gagccgctgt ccttcaagtg gtacgacaag
     2581 gaccgcatcg tcctcggcaa gcgcatggag gaccagctgc gcttcgccgt ctgctactgg
     2641 cattcgttct gctggaacgg gttcgacccg ttcggctacg acggcacgtt cgaacggccg
     2701 tggcaccgcg tggccgaccc gatggcggcg gccagggcca agctcgacgt gccctactac
     2761 tgcttccacg accgcgacgt cgcccccgaa ggggccacgc cgcgcgagag ccatgacaac
     2821 ttcctgcgcc tggtggacgt gctgggcgag aagcagcagg ccaccggccg caagctgctg
     2881 tggggcacgg ccaacctctt cagccaccgc cgcttcatgt cgggcgcggc cagcaacccc
     2941 gaccccgaga tcttcaagct cgccgccctg caggtgcgcg acgccatgga cgcgacgctc
     3001 aagctcggcg gcgagaacta tgtgctgtgg ggcggccgcg aaggctacga gaccctgctg
     3061 aacacgcgcc tggggcagga gctggaccag ctcggccgct tcctcaacat ggtcgtggag
     3121 cacaagcaca agatcggctt caagggcgcc atcctcatcg agcccaagcc gcgcgagccg
     3181 tccaagcacc agtacgactt cgacaccgcc accgtctacg gcttcctggt gcgctacggc
     3241 ctggagaagg aggtcaaggt caacatcgag gccaaccacg ccacgctctc gggccacagc
     3301 ttcgagcacg agatcgccac cgccggcgcc ctgggcatcc tgggcagcct ggacatgaac
     3361 cggggcgacc cgcagctggg ctgggacacc gaccagttcc ccaacaacgt gcccgagacg
     3421 gcaatggcgc tgtaccacgt gatccaggcc gggggcctcg gcagtggcgg gctgaatttc
     3481 gacgccaagg tgcgccgcca gagcatcgac gcggccgacc tcttccacgg ccacatcggc
     3541 ggcatggacg tctgcgccca ggccctgctg gtggccgaaa agatgatcaa cgacggccgt
     3601 ctcaaagccg ctgtggacag c
//
LOCUS       DATCRO010000903         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_145471, whole genome shotgun sequence.
ACCESSION   DATCRO010000903 DATCRO010000000
VERSION     DATCRO010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145471"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIN75_24595"
     CDS             <1..361
                     /locus_tag="VIN75_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8977416.1"
                     /translation="GFAAPEMYRRDGSLGPWTDIYAIGACLYACMQGYPPNDAPQRID
                     KDRLPLSLSRLRNVYSDNLIEVIEWCMALDPLSRPQSVFALQKELSREIERRYTTLSF
                     SERLKLQIENLKSGAHP"
     gene            398..1312
                     /locus_tag="VIN75_24600"
     CDS             398..1312
                     /locus_tag="VIN75_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY8977417.1"
                     /translation="MRFGTYQVSRKGGREKNEDRMGYCYTRDSGLFALADGMGGHPEG
                     EVASQLALQTIAATFQKEARTELKDPQRFLQDAILAGHHQLLRYATDRGLTDTPRTTI
                     VACVIQGGNIYWAHCGDSRLYIVRGDRLIARTRDHSYSELREALSHVVPLDEKVNRNV
                     LFTCLGSPGKPVIDSAGPMVLRTGDRVLLCSDGLWSSVEDPDIVKMLSEYPIHDSVPE
                     IVEQALRNAGEKSDNVTALAVEWEQTEDDEAAGNVSTRSLGEDVFASTIQASMSGDSF
                     LPDIDDDEIERSIREINEAIQRSGPRKT"
     gene            1518..2243
                     /gene="rph"
                     /locus_tag="VIN75_24605"
     CDS             1518..2243
                     /gene="rph"
                     /locus_tag="VIN75_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8977418.1"
                     /translation="MTFSRTQGRAANQLRPVKLTRDFTVHAEGSVLVEFGGTRVLCTA
                     SVEDRVPPFLKGKGEGWVTAEYGMLPRATHTRSDREAAKGKQSGRTQEIQRLIGRSLR
                     AVFDLRKLGERTITLDCDVLQADGGTRTAAITGACVAAHDAVAKLLAAGKLEHSPLNG
                     FVAAVSVGIVDGTPLLDLEYIEDSACDTDMNVVMTGAGHFIEVQGTAEGAAFSRTEMN
                     QLLDLADAGIRQLIAAQKSALAR"
BASE COUNT          415 a          857 c          798 g          341 t
ORIGIN      
        1 cggcttcgcc gcgcccgaga tgtaccgccg cgacggctcg ctgggcccgt ggaccgacat
       61 ctacgccatc ggcgcctgcc tgtacgcctg catgcagggc tatccgccca acgacgcgcc
      121 gcagcgcatc gacaaggatc gcctgccgct gtcgctctcg cggctgcgga acgtctattc
      181 ggacaacctc atcgaggtca tcgaatggtg catggcgctc gacccgctgt cccggccgca
      241 gagcgtgttc gcgctgcaga aggagctgtc gcgcgagatc gaacgccgct acaccacgct
      301 cagcttctcg gagcggctca agctgcagat cgagaacctg aagtccggag cccacccttg
      361 acgccctcga gcacgcgctc gggcctggag taagagaatg cggtttggaa cgtatcaagt
      421 gagccgcaag ggcggccggg agaagaacga agaccgcatg ggctattgct acacgcgcga
      481 ttcgggcctg ttcgcgctgg ccgacggcat gggcgggcat ccggagggcg aggtggcgtc
      541 gcagctcgcg ctgcagacga tcgcggccac gttccagaag gaggccagga ccgagctgaa
      601 ggatccgcag cgcttcctgc aggacgccat cctggccggc caccaccagc tcttgcgcta
      661 cgccaccgac cgcggcctga ccgacacgcc gcgcaccacc atcgtggcct gcgtcatcca
      721 gggcggcaac atctactggg cccactgcgg cgactcgcgc ctgtacatcg tgcgcggcga
      781 ccgcctcatc gcccgcacgc gcgaccactc gtactccgag ctgcgcgagg cgctgtcgca
      841 cgtcgtgccg ctggacgaga aggtcaaccg caacgtgctg ttcacctgcc tgggcagccc
      901 cggcaagccc gtcatcgaca gcgccggccc gatggtgctg cgcaccggcg accgagtgct
      961 gctgtgctcc gacggcctgt ggagcagcgt cgaggatccg gacatcgtca agatgctgtc
     1021 cgagtacccg atccacgact cggtgcccga gatcgtcgag caggccctgc gcaacgccgg
     1081 cgagaaaagc gacaacgtca ccgcgctggc cgtcgagtgg gagcagaccg aggacgacga
     1141 ggccgccggc aacgtgtcca cgcgctcgct cggcgaggac gtgttcgcgt ccaccatcca
     1201 ggccagcatg agcggagact cgttcctgcc cgacatcgac gacgacgaga tcgagcgctc
     1261 gatccgcgag atcaacgagg cgatccagcg ctcgggtccg cgcaagacct gagggcccga
     1321 ggggtctggc ctctcctcgt attcgcgcct aggcgtcgac agcaagcgtt gcgcctgccg
     1381 cgaatacgag gacaggcctg accccgatcc cccgcggaag ccggggtcag gcctgacctc
     1441 cgtccgcatt catcattgcc tcttcgctgc acgcttcccg cggacatcgg ataggccaga
     1501 ccccttagga cttgccaatg acgttctccc gcacccaggg ccgcgccgcc aaccagctgc
     1561 gtcccgtcaa gctcacgcgc gacttcaccg tgcacgccga aggctcggtg ctggtggaat
     1621 tcggcggcac gcgcgtgctt tgcaccgcgt ccgtcgagga ccgcgtgccg ccgttcctca
     1681 agggcaaggg cgagggttgg gtgacggccg aatacggcat gctgccgcgc gccacgcaca
     1741 cgcgctcgga tcgcgaggcg gccaagggca agcagagcgg ccgcacgcag gagatccagc
     1801 gcctgatcgg ccgctcgctg cgcgccgtgt tcgacctgag gaagctcggc gagcgcacca
     1861 tcacgctgga ctgcgacgtg ctgcaggccg acggcggcac gcgcaccgca gcgatcaccg
     1921 gcgcctgcgt cgcggcgcac gatgccgtgg ccaagctgct ggccgcaggc aagctggaac
     1981 actcgccact gaacggcttc gtcgcggcgg tgtcggtggg catcgtggac ggcacgccgc
     2041 tgctcgatct cgagtacatc gaagactcgg cctgcgacac cgacatgaac gtcgtcatga
     2101 ccggcgccgg ccacttcatc gaggtgcagg gcaccgccga gggcgccgcg ttctcgcgca
     2161 ccgagatgaa ccagctgctc gacctggccg acgccggcat ccgccagctg atcgccgcgc
     2221 agaagagcgc gctcgcacga tgaggacgtc gatgtcgatg atctcgcggg ccgtgcgccg
     2281 ggccgcccca aggaggcccg cggtcccctc gggggacgga gcgccatgcc cggcgcggga
     2341 ggggtcgtca tgaaactcgt cctcgcctcc aacaacgcca agaagctcgc cgaactgcag
     2401 gcgctgttcg a
//
LOCUS       DATCRO010000904         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_392105, whole genome shotgun sequence.
ACCESSION   DATCRO010000904 DATCRO010000000
VERSION     DATCRO010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392105"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="recA"
                     /locus_tag="VIN75_24610"
     CDS             complement(<1..925)
                     /gene="recA"
                     /locus_tag="VIN75_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8977419.1"
                     /translation="MDAPTKNASKTATPAAAPTAQSSALNAEKAKALQAALAQIEKQF
                     GKGSIMRLGDGEQAEDIQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQ
                     VAAEMQKVGGVCAFIDAEHALDINYAQKLGVNLQDLLISQPDTGEQALEIVDALVRSG
                     SVDLIVVDSVAALTPKAELEGEMGDSLPGLQARLMSQALRKLTGTIKKSNTMVIFINQ
                     IRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIKRGEEVVGNETKVKVVKNKVS
                     PPFKEAEFDILYGEGISREGEIIDLGVNAKVI"
     gene            1165..1836
                     /locus_tag="VIN75_24615"
     CDS             1165..1836
                     /locus_tag="VIN75_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8977420.1"
                     /translation="MRILIAEDDQVLADGLLRSLRGAGYAVDQVSSGTEADAALASHE
                     FDLLILDLGLPRLHGLEVLRKLRARGSALPVLILTAADSVEQRVKGLDLGADDFMAKP
                     FSLQELEARVRALTRRGLGTASSVIKHGPLTFDATGRVAYINDQMIDLSAREISLLEV
                     LLQRAGRLVSKDQLVTRLCEWGEEVSNNAIEVYIHRLRKKIEQGPIRIATVRGLGYCL
                     EKIAT"
     gene            1994..3484
                     /locus_tag="VIN75_24620"
     CDS             1994..3484
                     /locus_tag="VIN75_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8977421.1"
                     /translation="MTDRHEQRSLFGEILDWMLAPLLLLWPMSVALTWLVAQGIASRP
                     YDRELGQLAHSVAREMRADPAFERGLAGRHGLHLDEPTAALLRSDDVDRIWFQVRNAQ
                     GELAGGDAELAAPPEERAPADDVQFRDDTVHNDPVRVAYLWVGTASGTNSGVVVQVAE
                     TLDKRSRLATEIIKGVILPQFVILPLAVLLVWFALARGIAPLNALQQRIRRRQSQDLS
                     PLDEHDVPEEVAPLVRSINDLLARLDQTMGSQKHFLADAAHQMKTPLAGLRMQAELAQ
                     REIDAGGDPQAVKRSLRQIARSSQSAAHLVNQLLAMARAEDSEQARRQQDVPLIKLAR
                     EVVRDFVPRAMDKRIDLGYEGPDTDPAAPRVVGQPVLVRELIRNLVDNALQYTPAGGT
                     VTVRLMPDPYGQVIVLQVEDTGPGIPEAEREQVFQPFYRALGTDVDGSGLGLAIVKEI
                     ADKHAATVAVTSSTAASAPGEPAHGALFTVRFSCAPPPAPPAVTPT"
     gene            3705..5369
                     /locus_tag="VIN75_24625"
     CDS             3705..5369
                     /locus_tag="VIN75_24625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8977422.1"
                     /translation="MTPSTGAPAAVRATRLLAPLLEVAWIVLLVLPAATTGSVSRAFD
                     PDENTLFLVSCWFVVAARLLFPRRAFFLATLPVALFGVAYMATDFLRGADLLALLLQW
                     RTFSAVDIAGAARPYAWIALADTGALAVFAWACWRIVPGRAAGFRTRAAVFALTVAAA
                     VVVPPTTWLRAWPLDGALVLATTASNSKVMAQYLFPDSSTVDPRNPASRWNASRVAGA
                     PASETVVLVIGETIRNDFLRECGGPARMRPFAAGALVACDVTAGADGTDMSVPLLVSR
                     EMPGHRVRASDDATVVHALQEAGFEGHWISAQALAIAWPDAEHTVFAGQQGLDTALLL
                     PPLRAALARPAPLKAVVLHANNAHDPYCARYDPAHAPYPAACMDLGMEPSPANLAALR
                     DNYANAVDASVGFVNDVIAALDRQPQPAFLVFTPDHGENLLDDGRVIWGHSRRRPTQW
                     DTHVPAIFWANAAWRDAHAAQWARLRSQVRAPLMHADLVPTLLDAAGVRYDEPRTLPV
                     DLLARDVPPRTRIVQRSLDATIPWQTLVDEARAAGPRPATAMRSVR"
BASE COUNT          749 a         2044 c         1922 g          814 t
ORIGIN      
        1 cgatgacctt ggcgttgacg cccaggtcga tgatctcgcc ttcgcggctg atgccttcgc
       61 cgtacaggat gtcgaactcg gcttccttga acgggggcga gaccttgttc ttgacgacct
      121 tgaccttggt ctcgttgccg acgacttcct cgccgcgctt gatcgagccg atgcgacgga
      181 tgtccaggcg cacagaggcg tagaacttca gcgcgttgcc accggtggtg gtttccgggc
      241 tgccgaacat cacgccgatc ttcatgcgga tctggttgat gaagatcacc atcgtgttgc
      301 tcttcttgat ggtgccggtc agcttgcgaa gcgcctggct catcaggcgg gcctgcaggc
      361 cgggcagcga gtcgcccatc tcgccttcga gttcggcctt gggcgtcagc gcggcgaccg
      421 agtcgacgac gatcaggtcg accgagccgg agcgcaccag cgcgtcgacg atctcgagcg
      481 cctgctcgcc ggtgtcgggc tgcgagatca gcaggtcctg caggttgacg cccagcttct
      541 gcgcgtagtt gatgtccagc gcgtgttccg cgtcgatgaa ggcgcagacg ccgccgacct
      601 tctgcatctc ggccgcgacc tgcagcgtca gcgtggtctt gccggacgat tccgggccgt
      661 agatctcgac cacgcggccg cgcggcaggc cgccgacgcc cagcgcgatg tccaggccca
      721 gcgagccggt ggacacgacc tggatgtcct cggcctgctc gccgtcgccc aggcgcatga
      781 tggagccctt gccgaactgc ttctcgatct gggccagcgc ggcctgcagg gccttcgcct
      841 tctcggcgtt cagggccgag gactgcgcgg tcggcgcagc ggcgggggtg gcggtcttcg
      901 aggcgttctt ggtgggggcg tccatgcggg ttctccgtgt tcggtttggc gctggcgcga
      961 ttatgaccag ctactggtta tctgtacagg tgtcgaggaa agattattcg cgggctgagg
     1021 ctcgctgcgt gagccagccg caacagcttg cgcaaaatca atcctgcgcc acgcgggcgc
     1081 catgaccggg cggcatccgt cgcggaaaat ggtcgccagc gccaaaacaa gcggaaaccc
     1141 gcccgaagga ccaggagaca gtcgatgcgc attctgatcg ccgaagacga ccaggtcctc
     1201 gcggacggcc tgttgcgctc gctgcgcggc gcgggctatg ccgtcgacca ggtctccagc
     1261 ggcaccgagg ccgacgccgc cctcgcctcg cacgagttcg acctgctgat cctcgacctg
     1321 ggcctgccgc gcctgcacgg gctggaggtg ctgcgcaagc tgcgcgcgcg aggctccgcg
     1381 ctgccggtgc tgatcctgac cgcggccgac tcggtcgaac agcgcgtcaa gggcctggac
     1441 ctgggcgccg acgacttcat ggccaagccc ttctcgctgc aggagctcga ggcccgcgtg
     1501 cgcgcgctga cgcggcgcgg cctgggcacg gcctccagcg tcatcaagca cggcccgctc
     1561 acgttcgacg ccaccggccg cgtggcctac atcaacgacc agatgatcga cctgtccgcg
     1621 cgcgagatca gcctgctgga ggtgctgttg cagcgcgccg gccgcctcgt gagcaaggac
     1681 cagctcgtca cgcgcctgtg cgaatggggc gaggaagtga gcaacaacgc gatcgaggtc
     1741 tacatccacc ggctgcgcaa gaagatcgag cagggcccca tccgcatcgc caccgtgcgc
     1801 ggcctgggct actgcctcga gaagatcgcg acctgaagcg acagccgcag cctcctgcgc
     1861 tccggcacgg cgctgtcgtc cggcgaaccg gcgcgcttgc cggtggcgcg ccgcgacctc
     1921 ggcggccgcg gccccacgcc gccccggtcg gcgcgccgga catcgcgtcg gtcgcttatg
     1981 ctggccgtcg acgatgacgg acaggcacga acagcgttcc ctcttcggcg agatcctgga
     2041 ctggatgctc gcgccgctgc ttttgctgtg gccgatgagc gtggcgttga cctggctggt
     2101 ggcccagggc atcgcgagcc ggccgtacga ccgcgagctg ggccagctcg cccacagcgt
     2161 ggcccgcgag atgcgcgccg accccgcctt cgagcgcggc ctggccgggc gccacggcct
     2221 gcacctggac gagcccaccg ccgccctcct gcgcagcgac gacgtcgacc gcatctggtt
     2281 ccaggtgcgc aacgcgcagg gcgagctggc cggcggcgac gccgagctcg ccgcgccgcc
     2341 cgaggagcgc gccccggccg acgacgtgca gttccgcgac gacaccgtgc acaacgaccc
     2401 ggtgcgcgtc gcctacctgt gggtgggcac ggcctcgggc accaacagcg gcgtggtggt
     2461 gcaggtcgcc gagacgctgg acaagcgttc ccgcctggcc accgagatca tcaagggcgt
     2521 gatcctgccg cagttcgtca tcctgccgct ggccgtgctg ctggtgtggt tcgcgctggc
     2581 gcgcggcatc gcgccgctga acgcgctgca gcagcgcatc cgccgccgcc agagccagga
     2641 cctgagcccc ctggacgagc acgacgtgcc cgaggaggtg gcgccgctgg tgcgctcgat
     2701 caacgacctg ctcgcgcgcc tggaccagac gatgggctcg cagaagcact tcctggccga
     2761 cgcggcgcac cagatgaaga cgccgctggc cggcctgcgc atgcaggcgg agctcgcgca
     2821 gcgcgagatc gatgccggcg gcgacccgca ggccgtcaag cgctcgctca ggcagatcgc
     2881 gcgttccagc cagagcgccg cgcacctggt caaccagctg ctggcgatgg cgcgcgccga
     2941 ggactccgag caggcccgcc gccagcagga cgtgccgctg atcaagctgg cgcgcgaggt
     3001 ggtgcgcgac ttcgtgccgc gcgccatgga caagcgcatc gacctcggct acgaaggccc
     3061 ggacaccgat cccgcggccc cgcgcgtcgt gggccagccg gtgctcgtgc gcgagctcat
     3121 ccgcaacctg gtcgacaacg cgctgcagta cacgcccgcc ggaggcaccg tcaccgtgcg
     3181 cctgatgccc gacccgtacg gccaggtgat cgtgctgcag gtcgaggata cgggaccggg
     3241 catccccgag gccgagcgcg agcaggtgtt ccagccgttc taccgcgcgc tgggcaccga
     3301 cgtcgacggc agcggcctcg ggctggcgat cgtcaaggag atcgccgaca agcatgccgc
     3361 gaccgtcgcc gtcaccagct cgacggcggc gagcgcgccg ggcgaacccg cgcacggagc
     3421 cctgttcacc gtgagatttt cctgcgcgcc accgccggcg ccgcccgccg tgacgccgac
     3481 gtgaaggcgc cgggcggccg cagcccttat catccgcgac gcccaagaag agaacttccg
     3541 cgacgctcgt cgccgtgaag aagcccgggc cagacaggga gtctccattt gatgatcggc
     3601 catctgacgg caggcctggc cgcgccacgg acgtcgtgcg cgcccgcggc cctggccgtc
     3661 cgctcgcccg ccgcacgccg cgcgtccgcg cggggaccgg cgcgatgacg ccgtcgaccg
     3721 gcgccccggc ggccgtgcgc gccacgcgac tgctcgcgcc gctgctcgag gtcgcgtgga
     3781 tcgtgctgct ggtgctgccg gcggccacca cgggctcggt gagccgggcc ttcgaccccg
     3841 acgagaacac gctgttcctg gtgtcgtgct ggttcgtcgt ggcggcccgc ctgctgttcc
     3901 cgcgccgtgc gttcttcctc gcgacgctgc cggtggcgct gttcggcgtc gcctacatgg
     3961 ccaccgactt cctgcgcggc gccgacctgc tcgcgctgct gctgcaatgg cgcacgttct
     4021 cggccgtcga catcgcgggc gccgcgcggc cctatgcgtg gatcgcgctg gcggacaccg
     4081 gcgcgctggc cgtcttcgcc tgggcgtgct ggcgcatcgt gccgggccgc gcggccgggt
     4141 ttcgcacgcg cgcggccgtg ttcgcgctga ccgtggccgc cgccgtcgtc gtgccgccca
     4201 cgacctggct gcgcgcgtgg ccgctcgacg gcgcgctcgt gctggccacg accgcatcca
     4261 actcgaaggt gatggcgcag tacctgtttc ccgattcgtc cacggtcgat ccgcgcaacc
     4321 ccgcttcgcg ctggaacgcc tcgcgcgtgg ccggcgcgcc cgcgagcgag accgtggtgc
     4381 tggtcatcgg cgagaccatc cgcaacgact tcctgcgcga atgcggcggc ccggcgcgca
     4441 tgcggccgtt cgcggccggc gcgctggtcg cctgcgacgt caccgccggc gcggacggca
     4501 cggacatgtc ggtgccgctg ctggtgtcgc gcgagatgcc cggccaccgg gtgcgcgcgt
     4561 ccgacgacgc caccgtcgtc cacgcgctgc aggaagccgg cttcgagggc cactggatca
     4621 gcgcccaggc gctcgcgatc gcgtggcccg acgccgagca cacggtgttc gcgggccagc
     4681 agggcctgga cacggcgctg ctgctgccgc cgctgcgcgc ggcgctcgcg cggccggcgc
     4741 ccctgaaggc cgtcgtgctg cacgccaaca acgcccacga tccgtactgc gcccgctacg
     4801 accctgcgca cgcgccctac cccgcggcct gcatggacct cggcatggag ccgtcgcccg
     4861 ccaacctggc cgcactgcgc gacaactacg cgaacgcggt cgacgcgagc gtgggcttcg
     4921 tcaacgacgt cattgccgcg ctggaccgcc agccgcagcc ggccttcctg gtcttcacgc
     4981 ccgaccatgg cgagaacctg ctggacgacg gccgcgtcat ctggggccat tcgcgccgcc
     5041 gtccgaccca gtgggacacg cacgtgcccg ccatcttctg ggccaatgcg gcctggcgcg
     5101 acgcgcatgc cgcgcaatgg gccaggctgc ggtcgcaggt gcgcgcgccg ctgatgcacg
     5161 ccgacctggt gccgacgctg ctggacgccg ccggcgtgcg ctacgacgag ccgcgcacgc
     5221 tgccggtcga cctgctcgcg cgcgacgtgc cgccgcgcac gcgcatcgtg caacgcagcc
     5281 tggacgccac gatcccgtgg cagacgctgg tcgacgaggc ccgggccgcc ggcccgcgcc
     5341 cggcgacggc catgcgctcg gtgcgctgac atgcaagcgc aatcgtccgc caccggacct
     5401 tccgccgcct cgcaccccgc gcgcatcgcc gccgtcctgg gactgctgct ggcgaccgcc
     5461 tggctcggcc tgctctacat gccggcggcg atcaccttcc cgccggcgca gcactggaat
     5521 cgcggcgag
//
LOCUS       DATCRO010000905         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_966567, whole genome shotgun sequence.
ACCESSION   DATCRO010000905 DATCRO010000000
VERSION     DATCRO010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966567"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VIN75_24630"
     CDS             complement(<1..609)
                     /locus_tag="VIN75_24630"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8977423.1"
                     /translation="MNATIAAAALGVLLALATTAPRAQGMFRGGPAHQGVTSEAAPRA
                     FHRVKWTFPTGGRVVSSAVWSDGAVLFGSDDGNVYAVDAASGRQRWMARTGGPVASSP
                     AVADGRAFAVSYDGRLYAFDVHSGEVLWKFATEGERRFEAKGLHGLLPRNQTYPDLYD
                     VYLSSPLVVEGKVVFGSGDGHVYAVDAASGQLAWKFATGDVVH"
     gene            complement(606..1364)
                     /locus_tag="VIN75_24635"
     CDS             complement(606..1364)
                     /locus_tag="VIN75_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977424.1"
                     /translation="MSASAPWRPVASTLLLLAAGLARADSHAASDPIGRWEGVADIPG
                     QPLRLVIDLDRDAQGRWAGSVVLPGRGVKGTAVDGLAVDGCDVHLGLAAAFAGGDTLQ
                     PKLALACRSDGSLAGSFTLGGRAAPVSLHRSGPAQVDRPPAGRPITAALAGKWTGRYE
                     LGGFAREVTLTLANGADGNGAGQLVIVGKRTTTLQVDQVTQGREFVTLRASAAGFVIE
                     GRFAAQDGVIDGGVSQGPFEAALVLHRQPGEKAS"
     gene            complement(1487..>3020)
                     /locus_tag="VIN75_24640"
     CDS             complement(1487..>3020)
                     /locus_tag="VIN75_24640"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8977425.1"
                     /translation="RQAAGPSISLVDANDAKLLRAQLDERLDSLQVRQTDRKDYSGPA
                     LSLVDVIYMQFLHLPIPGRDGATAADVPKAWADIIARLEKGPAFIEAGQKLVTHPGKL
                     YGVAGKKQIDGAPDFLGGALTEAAKEQLAGDPAALERFVAARDAVLATLAKTAAFIQR
                     HVDGWPENFAMGRAAYDRMLQREQLLPYTGKDVEGMARDELAHGWAEEAWVKAEAAHR
                     KVALGADSGGGMAPSGPALIDYYRDRIAELRKFVVEHDVVTLPEWLGSLDVVETPKFQ
                     QPVSPGASMESPRLFSKSGNGHYFITPPESLKAAAERLDMNEDFDRDRILQTAAHEAM
                     PGHFLQLSIARRHSDYIRKIQYSGTFAEGWAFYGEEMFVRLGLYGDDLDARLFTARWE
                     RVRGARVIVDVKMASGEWTIAQAARFFEEQSGFTKSASEAAVAGYALRPGYVLNYTVG
                     RLQLEQLLGEYQSRMGDKGTLHDFHDRLLSYGSVPFAIVGPELLADLDKPAGAVRAVA
                     NY"
BASE COUNT          415 a         1104 c         1094 g          407 t
ORIGIN      
        1 gtgcacgacg tcgccggtgg cgaacttcca cgcgagctgg ccgctggcgg cgtcgaccgc
       61 gtagacgtgg ccgtcgccgc tgccgaagac gaccttgccc tccaccacca gcgggctcga
      121 aagatagacg tcgtacaggt cggggtacgt ctggttgcgc ggcagcaggc catggaggcc
      181 cttggcctcg aagcggcgct cgccctcggt ggcgaacttc cacagcacct cgcccgaatg
      241 cacgtcgaac gcgtagagac gcccgtcgta gctcaccgcg aaggcgcggc cgtccgccac
      301 cgccggcgac gacgccaccg ggccgccggt gcgggccatc cagcgctggc ggccactggc
      361 cgcgtcgacg gcgtagacgt tgccgtcgtc gctgccgaac agcaccgcgc catcgctcca
      421 gacggccgac gagacgacgc gcccgcccgt cgggaaggtc cacttcacgc gatggaatgc
      481 gcgcggcgcc gcctccgacg tgacgccctg gtgcgccggc ccgccgcgga acatgccctg
      541 cgcgcgcggc gcggtcgtgg cgagcgccag cagcacgccg agcgcggcgg cggcgatcgt
      601 cgcgttcatg aggccttctc ccccggttgg cgatgcagca cgagcgcggc ctcgaacggc
      661 ccctgcgaca cgccgccgtc gatcacgccg tcctgcgccg cgaagcggcc ctcgatgacg
      721 aagccggcgg cgctcgcgcg caacgtgacg aactcgcggc cctgcgtgac ctggtcgacc
      781 tgcagcgtgg tcgtgcgctt gcccacgatc accagctggc cggcgccgtt gccgtcggcg
      841 ccgttggcca gcgtcagggt cacctcgcgc gcgaagccgc ccagctcgta gcggcccgtc
      901 cacttgccgg ccagcgccgc ggtgatggga cgtcccgccg ggggccggtc gacctgcgcg
      961 ggcccgctgc gatgcaggct caccggggcg gcgcggccgc cgagcgtgaa gctgccggcg
     1021 agcgacccat cggatcggca cgccagcgcg agcttcggtt gcagcgtgtc gccgccggca
     1081 aatgcggcgg ccaaccccag gtggacatcg cagccgtcga cggccaggcc gtcgacggcc
     1141 gtgcccttga cgccgcgccc cggcaacacc accgagccgg cccaacggcc ctgcgcgtcg
     1201 cgatcgaggt cgatcaccag ccgcagcggc tggcccggga tgtcggcgac gccttcccag
     1261 cggccgatgg gatcggacgc cgcgtgcgag tccgcgcggg ccaggcccgc ggcgagcagc
     1321 aggagcgtcg aggcgactgg gcgccaggga gcggatgcgg acatgcggcc tccacgatca
     1381 acgggatggc tcgcatgatg gaagcgcccg cggcgccgcg gggcgccgat gcgacgaatg
     1441 ggggaaacgg cgccgcgagc agcggtgccg tcgccctggc ggatcgtcag tagttcgcga
     1501 cggcgcgcac cgcgccggcg ggcttgtcca ggtcggccag cagctcgggc ccgacgatgg
     1561 cgaacggcac cgagccgtac gacagcaggc ggtcgtggaa gtcgtgcagc gtgcccttgt
     1621 cgcccatgcg gctctggtac tcgcccagca gctgctccag ctgcagccgg cccaccgtgt
     1681 agttcagcac gtagcccggg cgcagcgcgt agccggccac cgcggcctcg gacgcggact
     1741 tcgtgaagcc ggactgctcc tcgaagaacc tggcggcctg cgcgatcgtc cattcgccgc
     1801 tggccatctt cacgtcgacg atgacgcggg cgccgcgcac gcgctcccag cgcgcggtga
     1861 acaggcgcgc gtcgaggtcg tcgccgtaca gccccaggcg cacgaacatc tcctcgccgt
     1921 agaacgccca gccctcggcg aaggtgcccg agtactggat cttgcggatg tagtccgagt
     1981 ggcggcgcgc gatcgacagc tgcaggaagt ggcccggcat cgcctcgtgg gcggcggtct
     2041 gcaggatgcg gtcgcggtcg aagtcctcgt tcatgtcgag acgctcggct gcggccttca
     2101 gcgactcggg cggcgtgatg aagtagtggc cgttgcccga cttcgagaac aggcgcggcg
     2161 actccatcga cgcgcccggc gacaccggct gctggaactt gggcgtctcg acgacatcca
     2221 gcgaaccgag ccattccggc agggtgacga cgtcgtgctc cacgacgaac ttgcgcagct
     2281 cggcgatgcg gtcgcggtag tagtcgatca gggccgggcc gctgggcgcc atgccgccgc
     2341 cgctgtcggc gccgagcgcc accttgcgat gcgccgcctc ggccttgacc caggcctcct
     2401 cggcccagcc atgcgccagc tcgtcgcggg ccatgccctc gacgtccttg cccgtgtagg
     2461 gcagcagctg ttcgcgctgc agcatccggt cgtaggccgc gcggcccatc gcgaagttct
     2521 cgggccagcc gtcgacgtgc cgctggatga acgccgcggt cttggcgagc gtggccagca
     2581 ccgcgtcgcg cgccgcgacg aagcgctcga gcgcggccgg gtcgcccgcg agctgctcct
     2641 tggccgcctc ggtgagcgcg ccgcccagga aatcgggcgc gccatcgatc tgcttcttgc
     2701 cggccacgcc gtacagcttg cccggatgcg tcaccagctt ctggccggcc tcgatgaacg
     2761 cggggccctt ctcgaggcgg gcgatgatgt cggcccaggc cttgggcacg tcggcggcgg
     2821 tggccccgtc gcggccgggg atcggcaggt gcaggaactg catgtagatc acgtcgacca
     2881 ggctcagcgc ggggccggaa tagtccttgc ggtcggtctg gcgcacctgc aggctgtcca
     2941 ggcgctcgtc gagctgcgcg cgcagcagct tcgcgtcgtt cgcgtcgacc agcgagatcg
     3001 acgggcccgc ggcctggcgg
//
LOCUS       DATCRO010000906         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_77592, whole genome shotgun sequence.
ACCESSION   DATCRO010000906 DATCRO010000000
VERSION     DATCRO010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77592"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VIN75_24645"
     CDS             complement(<1..1659)
                     /locus_tag="VIN75_24645"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY8977426.1"
                     /translation="MNAKGTGAAVVCAVMAAGCGGSGGDSTPPAPQASYVSPSVYVQG
                     QAITPLAPTVTGTATGFSVSPALPAGLAMDAATGVISGTPGTGAPTTDYTVTVTGAGG
                     STTAHVHLDVEPVATVTRSAVTGTSVYPTVTLDATALGLTGTVYAKVADAGGTFGPTV
                     TVSANGSSRVLEFTSIGSVAPGAHTGQATVSLCQDAACSTPQAVPTLLVNYSLDVRSA
                     GGAWPGNHLTTLSAIAGAPEWATFQGNAAHTGLVPVTLDPNQFGARWQLGVPSFLYFN
                     GLFNLATVTTAGGNFYIAGNNAVTAHSEYDGSTLWSYSFAGLPFPSVNPPAVSNGTVY
                     VAAGQQSSTYMFGLDASNGSVKFRSAMSSQWEDYLAPTVGPSGVYTNAGGYGGVYGFD
                     PQGTQLFFGGTAQQSAWTPAVDASGVYAYTGDALHVFDPVTGAVRASIADPTFTNYIY
                     EIGGSAVLGAPNSVFAAAYGNSWLNGGGIGNALVHFNLTSNSVDWSVHGVYPSTPAYD
                     GAVVYVANNNPLRVEARAEADGSLIWSWTPPAAGDTKFLSEVLLT"
     gene            complement(1779..>2570)
                     /locus_tag="VIN75_24650"
     CDS             complement(1779..>2570)
                     /locus_tag="VIN75_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8977427.1"
                     /translation="LHAPRVLEQDVHQGFLLLDDLGSTQYLAALRELDHAGADLLMRD
                     AIGALIRWQLHGDCSTLPPYDDALLRREIQLFPDWCVTREFEVTWTARENEIWKTVSQ
                     RLIDSALAQPQVAVHRDWMPRNLMVCDRNPGVLDFQDAVRGPITYDVASMLRDAFISW
                     DEEHELDWAVRYWQQARHAGLPVDEDFGEFWRQTEWMGLQRHLKILGIFCRLKHRDGK
                     PHYSEDLPRFFNYATKVANRYGPLRPLLNLLEPLTGAGVQAGYTF"
BASE COUNT          361 a          852 c          957 g          400 t
ORIGIN      
        1 ggtcagcagg acctcgctga ggaacttcgt gtcgcccgcg gccggcggtg tccacgacca
       61 gatcagcgac ccgtcggcct cggcgcgcgc ttccacccgc aacgggttgt tgttggcgac
      121 gtagacgacc gcaccatcgt aggcgggcgt ggacgggtag acgccgtgga ccgaccagtc
      181 gaccgagttg gacgtcaggt tgaagtgcac gagcgcgttg ccgatgccgc cgccgttcag
      241 ccagctgttg ccgtaggccg cggcgaacac ggagttcggt gcgcccagca ccgccgagcc
      301 gccgatctcg tagatgtagt tcgtgaaggt cgggtcggca atgcttgccc tgaccgcgcc
      361 ggtcaccgga tcgaacacgt gcagcgcgtc gcctgtgtag gcgtagacgc cgctggcgtc
      421 gaccgcgggt gtccaggcgg actgctgcgc cgtgccgccg aagaacagct gcgtgccctg
      481 cggatcgaac ccgtagacgc cgccgtagcc gcccgcgttg gtgtagacgc ccgacgggcc
      541 gacggtcggc gccaggtagt cctcccactg cgacgacatc gccgagcgga acttgaccga
      601 gccgttgctg gcgtccaggc cgaacatgta ggtcgacgat tgctggcccg cggccacgta
      661 cacggtgcca ttgctgaccg cgggcgggtt caccgacggg aagggcaggc cggcgaagct
      721 gtagctccag agggtcgagc cgtcgtattc gctgtgcgcc gtgacggcgt tgttgccggc
      781 gatgtagaag ttcccaccgg cggtggtcac cgtcgcgagg ttgaacaggc cgttgaagta
      841 gaggaacgag gggacgccga gctgccagcg cgcgccgaac tggttcgggt cgagcgtgac
      901 agggacgaga cccgtgtgcg cggcattgcc ctggaacgtg gcccactcgg gcgcgcccgc
      961 gatggccgac agcgtcgtca ggtggttgcc gggccaggcg ccgccggcgc tgcgcacgtc
     1021 caggctgtag ttgaccagca gcgtcggcac cgcctgcggc gtgctgcagg cggcgtcctg
     1081 gcacaggctc accgtcgcct ggccggtgtg cgcgcccggc gccacgctgc cgattgacgt
     1141 gaattcaagc actcggctgg agccgttggc gctgacggtg accgtcgggc cgaaggtgcc
     1201 gccggcatcg gcgaccttgg cgtagaccgt gccggtcagg cccagcgcgg tggcgtcgag
     1261 cgtcaccgtc ggatagaccg aggtgcccgt caccgcgctg cgcgtgacgg tggccaccgg
     1321 ttcgacgtcc aggtgcacgt gggccgtggt cgatccgccc gcgcccgtga ccgtgaccgt
     1381 gtagtcggtc gtgggggctc cggtgccggg cgtgccggag atgacgccgg tggcggcgtc
     1441 catggccagg cccgcgggca gcgcgggcga cacgctgaac ccggtggcgg tgccggtgac
     1501 ggtgggtgcc agcggcgtga tggcctggcc ctggacgtag accgacggcg agacatacga
     1561 cgcctgcggc gccggaggcg tcgaatcccc gccgcttccg ccgcaacccg cggccatgac
     1621 ggcgcagacg accgccgctc ctgttccctt agcgttcatg acgctctccc tgtgttgttg
     1681 gatggtggcg cctgcaaggg cgccgcgcgc attctatggt cgagcaggag tcggggcccg
     1741 gccgtcgcgc gatcgtccgc ccgcggccgg cccgggagtc agaacgtgta gccggcctgc
     1801 acgccggcgc cggtcagcgg ttccagcagg ttcagcagcg ggcgcagcgg gccgtagcgg
     1861 ttggccacct tggtcgcata gttgaagaag cgcggcaggt cctcgctgta gtgcggcttg
     1921 ccgtcgcggt gcttcaggcg gcagaagatg cccaggatct tgaggtggcg ctgcaggccc
     1981 atccattcgg tctggcgcca gaactcgccg aagtcctcgt ccaccggcag gccggcgtgg
     2041 cgcgcctgct gccagtagcg cacggcccag tccagctcgt gttcctcgtc ccacgagata
     2101 aaggcgtcgc gcagcatcga ggcgacgtcg taggtgatcg ggccgcgcac ggcgtcctgg
     2161 aagtccagca cgccggggtt gcggtcgcac accatcaggt tgcgcggcat ccagtcgcga
     2221 tgcaccgcca cctggggctg ggcgagcgcg ctgtcgatca ggcgctggct gacggtcttc
     2281 cagatctcgt tttccctggc cgtccaggtg acctcgaact cgcgcgtgac gcaccagtcg
     2341 gggaacagct ggatctcgcg gcgcagcagc gcgtcgtcgt acggcggcag cgtgctgcag
     2401 tcgccgtgca gctgccaccg gatcagcgcg ccgatcgcgt cgcgcatcag caggtcggcg
     2461 ccggcgtggt cgagctcgcg cagcgcggcc aggtattgcg tgctgccgag gtcgtccagc
     2521 agcaggaagc cctggtggac gtcctgctcg aggacgcgcg gggcgtgcag
//
LOCUS       DATCRO010000907         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_201641, whole genome shotgun sequence.
ACCESSION   DATCRO010000907 DATCRO010000000
VERSION     DATCRO010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201641"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIN75_24655"
     CDS             complement(<1..357)
                     /locus_tag="VIN75_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8977428.1"
                     /translation="MKAIGYRQNLPVDQPHALEDLELPAPQPGPRDLLVRVKAVSVNP
                     VDTKVRRNRAPQAGRPEVLGWDAVGIVEGIGADVQGFRPGDRVFYAGSITRPGANSEL
                     HVVDERIAAKAPASLED"
     gene            441..1355
                     /locus_tag="VIN75_24660"
     CDS             441..1355
                     /locus_tag="VIN75_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977429.1"
                     /translation="MKIENIADLQVLVHTARGGTLTAAARTLGITPAAASATLKRLES
                     QLGARLFERSTRAMRLTPQGQILLDYAARAFELLDEGQSQVHADRATLVGTVRVAAPS
                     DLTRNVLLPLLDEFMLAHPGLQLQLSVADRVLDVTRDEVDVAIRYGALPDSRLVARLL
                     AEAWPICSASPEYLRRHGTPRTPQELARHNCITFDRGGRRHRGWRFWRDGQWTEVRVD
                     GDRSVDDASLAREWAIAGRGIILKSEIDQRRDLASGALVPLLAEWRTEPYPLHALLPS
                     GRFVPNRVRALVDFLVERFSAGPGASAS"
     gene            complement(1324..2691)
                     /locus_tag="VIN75_24665"
     CDS             complement(1324..2691)
                     /locus_tag="VIN75_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8977430.1"
                     /translation="MSDAPETGRVLLVDDEPAFQRLGGSFLRDLGHAVTVAGDGEQAL
                     AAFERERPELVLLDLAMPPHMDPEIGLEMIPRLPGVPVIVLTGHGEHELALRATELGA
                     WDFITKPIDPDMLRFIVARALRKARLDAEVATLRAATATDDLGLVGQAPAMAQLRAMV
                     RRVAQTSVSVLVLGPTGTGKELVARALHACGARSAGPFVAIHCGALSAELLESELFGH
                     MKGSFTGAYRDQPGLVEAAHGGTLFLDEVGEMPAPMQVKLLRFLQEGTFVPVGGRVQK
                     RAEARVVAATHRDLEAMVRDGSFREDLFYRLKGVVLRTPALAERPADVPQLATRFLHR
                     VAPRAGFTAGALAWLTAREWPGNVRELKAVVESAGALILPGCNEVDAELLRLASGDTA
                     DAGAPPGPVDASSGPLEAALNELELRLVRDAMTAAGGNQSEAARRLGVSRVGLIKKLT
                     RLGLR"
     gene            complement(2827..>3199)
                     /locus_tag="VIN75_24670"
     CDS             complement(2827..>3199)
                     /locus_tag="VIN75_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977431.1"
                     /translation="LDASLEGELLVDADARRVDQALTNLFENARVAAGAEDPAPGQVH
                     VAAEHVPGALLLHVCDDGPGVPAEIRARLFEPFASRSPGGTGLGLAIVARIMNAHGGS
                     VALTERPPWRTCFTLSFPTTP"
BASE COUNT          409 a         1174 c         1187 g          429 t
ORIGIN      
        1 gtcctcgagc gacgcgggcg ccttcgcggc gatgcgctcg tcgacgacgt gcagctcgct
       61 gttggcgccg ggccgcgtga tcgagccggc gtagaacacg cggtctcccg gccggaagcc
      121 ctgcacgtcg gcgccgatgc cctcgacgat gccgacggcg tcccacccga gcacctcggg
      181 tcggccggcc tgcggggcgc ggttgcggcg caccttggtg tcgacggggt tgaccgagac
      241 ggccttgacg cgcacgagca ggtcgcgcgg gccgggctgc ggcgcgggca gctcgaggtc
      301 ctcgagggcg tggggctggt ccacgggcag gttctggcgg tatccgatgg ctttcatggc
      361 gaactccggc ttgcgatgac cgttatcgta ggaacgcaag cctggattga taagatggct
      421 aggaagcgga tcactttcaa atgaagattg aaaatatcgc cgacctgcag gtgctggtgc
      481 acaccgcccg cggcggcacg ctgaccgccg cggcgcggac gctgggcatc acgccggcgg
      541 ccgccagcgc gacgctcaag cgcctggagt cgcagctggg cgcgcggctg ttcgagcgct
      601 ccacgcgcgc gatgcgcctg acgccccagg gccagatcct gctggactac gcggcgcgcg
      661 cgttcgagct gctggacgag ggccagtcgc aggtgcacgc cgaccgtgcc acgctggtgg
      721 gcaccgtgcg cgtggccgcg ccgtccgacc tcacgcgcaa cgttctgctg ccgctgctgg
      781 acgagttcat gctggcgcac cccggcctgc agctgcagct gtccgtggcc gaccgcgtgc
      841 tggacgtgac gcgcgacgag gtcgatgtcg ccatccgcta cggcgcgctc cccgactcgc
      901 gcctggtcgc gcgcctgctg gccgaggcct ggccgatctg cagcgcgtcg cccgagtacc
      961 tgcgccgcca cggcaccccg cgcacgccgc aggagcttgc gcggcacaac tgcatcacct
     1021 tcgaccgtgg cggccgccgc catcgcggct ggcgcttctg gcgcgacggc cagtggacgg
     1081 aggtgcgcgt ggacggcgac cgcagcgtcg acgacgcgtc gctggcgcgc gagtgggcga
     1141 tcgcggggcg cggcatcatc ctgaagtcgg agatcgacca gcgccgcgac ctcgccagcg
     1201 gcgcgctggt gccgctgctc gccgagtggc gaaccgagcc ctatccgctg cacgcgctgc
     1261 tgcccagcgg ccgcttcgtg cccaaccggg tgcgggcgct ggtggacttc ctcgtcgaac
     1321 gcttcagcgc aggcccaggc gcgtcagctt cttgatcagc ccgacgcgcg acacgcccag
     1381 ccgccgcgcc gcctccgact ggttgccgcc tgccgcggtc atcgcgtcgc gcacgaggcg
     1441 cagctcgagc tcgttgagcg cggcctccag cgggccgctc gatgcgtcga ccgggcccgg
     1501 cggcgcgccg gcgtcggccg tgtcgccgct ggccaggcgc agcagctcgg cgtcgacctc
     1561 gttgcagccc ggcaggatca gcgcgccggc cgattcgact accgccttga gctcgcgcac
     1621 gttgccgggc cactcgcgcg ccgtcagcca cgcgagcgcg ccggccgtga acccggcgcg
     1681 cggcgccacg cggtgcagga agcgcgtggc cagctgcggc acgtcggcgg ggcgctcggc
     1741 gagcgccggc gtgcgcagca cgacgccctt gaggcgatag aacaggtcct cgcggaagct
     1801 gccgtcgcgc accatcgcct cgaggtcgcg gtgcgtggcc gcgacgacgc gggcctcggc
     1861 ccgcttctgc acgcgtccgc ccaccggcac gaaggtgcct tcctgcagga agcgcagcag
     1921 cttgacctgc atcggcgcgg gcatctcgcc gacctcgtcg aggaacagcg tgccgccgtg
     1981 cgcggcctcc accaagccgg gctggtcgcg gtaggcgccg gtgaagctgc ccttcatgtg
     2041 gccgaacagc tcgctctcca gcagctcggc cgacagcgcg ccgcaatgga tcgccacgaa
     2101 cggccccgcg ctgcgcgcgc cgcacgcgtg cagcgcgcgc gcgaccagtt ccttgccggt
     2161 gcccgtcggc cccagcacca gcacgctgac ggaggtctgc gccacgcgcc gcaccatcgc
     2221 gcgcagctgc gccatggcgg gcgcctggcc gaccaggccg aggtcgtccg tggcggtggc
     2281 cgcgcgcagc gtcgcgacct ccgcgtcgag ccgcgccttg cgcagcgcgc gcgccacgat
     2341 gaagcgcagc atgtcgggat cgatcggctt ggtgatgaag tcccacgcgc cgagctcggt
     2401 ggcgcgcagt gcgagctcgt gctcaccgtg gccggtcagc acgatgacgg gcacgccggg
     2461 caggcgcggg atcatctcca ggccgatctc cggatccatg tgcggcggca tcgccaggtc
     2521 cagcagcacc agctcgggcc gctcgcgctc gaaggcggcc agcgcctgct cgccgtcgcc
     2581 ggccaccgtg acggcgtggc cgaggtcgcg caggaacgag ccgcccaggc gctggaaggc
     2641 cggctcgtcg tcgacgagca gcacgcggcc ggtttcaggt gcatcgctca tcaggaaacc
     2701 tccgccctgc gggctgcggt gtgaccgacg caggagggaa cgccttcggg cggccgtgcg
     2761 tcgctcatca gtcaacctcc gccctgcggg ctgcggtgcg atcgccttcg ggcggccgtg
     2821 cgtcgatcat ggcgtggtcg ggaacgagag ggtgaagcag gtgcgccagg gcgggcgctc
     2881 ggtcagcgcc accgagccgc cgtgcgcgtt catgatgcgc gccacgatcg ccaggcccag
     2941 gccggtgccg ccggggctgc gcgaggcgaa gggctcgaac aggcgggcgc ggatctcggc
     3001 gggcacgccg gggccgtcgt cgcatacgtg cagcagcagt gcgccgggca cgtgttcggc
     3061 cgcgacgtgc acctggcccg gcgccgggtc ctcggcgccg gccgcgacgc gcgcgttctc
     3121 gaacaggttg gtcagcgcct ggtcgacgcg gcgcgcgtcg gcgtcgacca gcagctcgcc
     3181 ctcgaggctc gcgtccagg
//
LOCUS       DATCRO010000908         5046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_223765, whole genome shotgun sequence.
ACCESSION   DATCRO010000908 DATCRO010000000
VERSION     DATCRO010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223765"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VIN75_24675"
     CDS             complement(<1..769)
                     /locus_tag="VIN75_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY8977432.1"
                     /translation="MNPAHTDAAPELWVGAECTINRVGDRWRDQAELTGFRHRVEDDI
                     DRIASLGARRVRLPVLWEHVAQRGTGRLDFGWSDRALARLRERGLGAIVGLLHHGSGP
                     AHTNLLAPRFPMLLADYARRVAERYPDQRLWTPVNEPLTTARFSGLYGLWYPHAADDR
                     SFVRALLHQVQATVLAMRAIREVVPDAQLVQTDDLGYTRCTPALAAQAAFDNERRWLA
                     FDLLAGRVDASHPLRDWLCGCGAGEDELDRLVHSPCPP"
     gene            complement(766..1971)
                     /gene="glf"
                     /locus_tag="VIN75_24680"
     CDS             complement(766..1971)
                     /gene="glf"
                     /locus_tag="VIN75_24680"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY8977433.1"
                     /translation="MKPQDTELRTAARMGSGFDTLVVGAGFAGSVIAERLASQLGQRV
                     LVVDKRPHVGGNAYDRFDDEGLLIHPYGPHIFHTNSADVFDHLSKFTAWRPYQHRVLA
                     SVDGQAVPMPINLDTVNRLYGLNLTSFQLEDFFASVAEKPAAIRTSEDVVVAKVGREL
                     YNKFFRGYTRKQWGLDPSELDASVTARVPTRTNRDDRYFTDTYQAMPLQGYTRMFERM
                     LAHPNIKVMLNTDYREVAELVPWRHMVYTGPIDAFFDFRHGRLPYRSLEFRHETLQQA
                     QFQPVGTVNYPNDFAYTRISEFKHLTGQRHHATSIVYEYPRAEGDPYYPVPRAENTAM
                     YRLYEDEAEKLQDVSFVGRLATYKYYNMDQVVAQSLTLFKKLAARHAERARHGEAALT
                     AAPELVPAK"
     gene            complement(2078..2755)
                     /locus_tag="VIN75_24685"
     CDS             complement(2078..2755)
                     /locus_tag="VIN75_24685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8977434.1"
                     /translation="MTDAHITDNAPAPAPAADGSPSRRERILESAARAFATHGYRGSS
                     LRDIAREAGCSLTLLDHHFGGKSQLLDAVLQQQHQNCEKRLDGLRASIESAPELGLED
                     FVERWANYEFDLYTTPQGRLYLQLMMRLSMESEVESQRRRDLDCAQVLVLKAFARARP
                     ALDDQARQAGWFLASAALYAAIVRADEGTPPEGVTESTPERRRTIAFMVEGLKGCWNE
                     RPVASAG"
     gene            complement(2762..3556)
                     /locus_tag="VIN75_24690"
     CDS             complement(2762..3556)
                     /locus_tag="VIN75_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8977435.1"
                     /translation="MASLNPERPLAVVTGASSGIGRELARLAAQDGHDLVIAADQGPL
                     DDAAAAFRALGANVEAVEADLSTSFGVDKLMLAVGGRPVDVLVANAGHGLGHGFLDKP
                     FNEAKHVVDTNITGTLDLLHRVGTGMRQRGRGRILITGSIAGLMPGSYQAVYNGTKAF
                     IDSFSFALRNELKDTGISVTCLMPGATETHFFERADMLDTKVGSEEKASPAEVAEEGW
                     HAMKKGQDHVVAGWKNKVSAAMTNVVPDSMLAEQHRKMAEPGSARH"
     gene            complement(3574..4746)
                     /locus_tag="VIN75_24695"
     CDS             complement(3574..4746)
                     /locus_tag="VIN75_24695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY8977436.1"
                     /translation="MKALTYHGSKDVRVETVPDPRLVEPDDILLRVTATAICGSDLHI
                     YRGKIPMMESGDILGHEFMGIVEETGPAVTRVKRGDRVVIPFTISCGQCFFCNRKLFA
                     ACETTNPDRGAIINKKDTRAGAALFGYSHLYGGIPGGQAEFVRVPKANVGPLVIPDAG
                     LEDKQVLFLSDILPTGYMAAVNAAVGKGSSVAIFGAGPVGLMAAASARLLGAERIFMV
                     DHHQYRLEFARRAYGVEPLNFDEVDDAANEIIDRMDGRGVDASIDAVGFEAKGSAVET
                     ALTAIKLEGSSGASIRQAIAATRRGGAISVPGVYAGWIHGFLFGDVFEKGLSIKGGQT
                     HAQNYMPQLLEHIARGDLHPEAIISHTLPLEQAAHGYEIFEKKQQDCRKVVLTTVH"
BASE COUNT          724 a         1680 c         1819 g          823 t
ORIGIN      
        1 cgggcggaca gggcgaatgg accaggcgat cgagctcgtc ctcgcccgcg ccgcaaccgc
       61 acagccagtc gcgcagcgga tgcgacgcgt ccacccgtcc cgcgagcagg tcgaacgcga
      121 gccagcggcg ctcgttgtcg aacgcggcct gcgccgcgag cgccggcgtg cagcgcgtgt
      181 agccgaggtc gtcggtctgc accagctgcg cgtcggggac cacctcgcgg atcgcgcgca
      241 tggccagcac cgtcgcctgc acctggtgca gcagcgcccg cacgaagctg cggtcatccg
      301 cggcatgcgg ataccacagc ccgtacaggc cggagaagcg cgccgtcgtc agcggctcgt
      361 tgacgggcgt ccagaggcgc tggtcgggat agcgttcggc gacgcggcgc gcgtaatccg
      421 caagcagcat cgggaagcgc ggcgcgagca ggttcgtgtg cgccggtccg ctgccgtggt
      481 gcagcaggcc gacgatcgca ccgagcccgc gctcgcgcag gcgggcgagg gcgcgatcgg
      541 accacccgaa gtcgaggcgg ccggtgccac gctgggcgac gtgctcccac agcaccggca
      601 gccgcacgcg gcgcgcgccg agcgacgcga tccggtcgat gtcgtcctcg acgcgatggc
      661 gaaagccggt gagctcggcc tggtcgcgcc agcggtcgcc gacgcggttg atcgtgcact
      721 ccgcgccgac ccacagctcc ggcgcggcgt ccgtgtgcgc gggattcact tggcaggaac
      781 cagctcgggc gcggcggtca acgccgcttc gccgtggcgg gcgcgctccg cgtgccgcgc
      841 cgcgagcttc ttgaacagcg tcagcgactg ggccacgacc tggtccatgt tgtagtactt
      901 gtaggtcgcg aggcgcccca cgaagctcac gtcctgcagc ttctcggcct cgtcttcgta
      961 cagccggtac atcgccgtgt tctccgcgcg cggcaccggg tagtacggat cgccttcggc
     1021 gcgcgggtat tcgtagacga tggacgtcgc atgatggcgc tgtccggtga gatgcttgaa
     1081 ctcgctgatg cgcgtgtagg cgaagtcgtt cgggtagttg acggtgccca ccggctggaa
     1141 ctgcgcctgc tgcagcgtct cgtggcgaaa ttcgaggctg cgatagggca gcctcccgtg
     1201 gcggaagtcg aagaaggcgt cgatcgggcc ggtatagacc atgtgccgcc agggcacgag
     1261 ttcggcgacc tcgcggtagt cggtgttgag catcaccttg atgttcgggt gcgccagcat
     1321 gcgttcgaac atgcgcgtgt agccttgcag cggcatcgcc tggtaggtgt cggtgaagta
     1381 gcggtcgtcg cggttggtgc gcgtgggcac ccgcgccgtg acgctggcgt cgagctcgga
     1441 tggatcgagc ccccactgct tgcgcgtata gccgcggaag aacttgttgt acagctcgcg
     1501 gcccaccttg gccacgacga cgtcctccga ggtcctgatc gccgccggct tctcggccac
     1561 cgacgcgaag aagtcctcga gctggaagct cgtgaggttc aggccgtaga ggcgattgac
     1621 cgtgtccagg ttgatcggca tcggcaccgc ctgcccgtcg acgctggcga gcacgcggtg
     1681 ctggtagggc cgccacgcag tgaacttcga gaggtgatcg aagacgtcgg cggaattcgt
     1741 gtgaaagatg tgcgggccgt atggatggat caacaggccc tcgtcgtcga agcggtcgta
     1801 ggcgttgccg ccgacatggg gccgcttgtc gaccacgagg acccgctggc ccagctggga
     1861 cgccagtcgt tcggcgatca cgctgccggc gaagccggcg ccgaccacga gggtgtcgaa
     1921 gccggagccc atgcgtgccg cggttctcag ttctgtgtct tggggtttca tcttgtcggg
     1981 tctccttgag cgtgtgcccc ccaagcaacg cctgttccta acggttacat gcagcggaca
     2041 aacgcatggc gagcgggcat gaaggcccgc aggagggtca gccggcggat gcgacgggac
     2101 gttcgttcca gcagcccttg aggccctcga ccatgaaggc gatggtgcgc cgccgctcgg
     2161 gcgtcgactc ggtcacgcct tcgggtggcg tgccctcgtc cgcccgcacg atggcggcgt
     2221 acagcgccgc gctggccagg aaccagccgg cctggcgcgc ctggtcgtcc agggccggac
     2281 gcgcccgcgc gaaggccttg agcacgagca cctgcgcgca gtcgaggtcg cgccggcgct
     2341 gcgactcgac ctcggactcc atcgacagcc gcatcatcag ctgcaggtaa agccggccct
     2401 gcggggtcgt gtacaggtcg aactcgtagt tggcccagcg ctcgacgaag tcctcgagcc
     2461 cgagctccgg cgcggactcg atgctggccc gcaggccgtc gagccgcttc tcgcagttct
     2521 ggtgctgctg ctgcaggacg gcgtccagca gctggctctt gccgccgaaa tggtggtcga
     2581 gcagcgtcag cgagcagccg gcctcgcgcg cgatgtcgcg caggctgctg ccgcgatagc
     2641 cgtgggtggc gaacgcgcgc gcggccgatt cgaggatgcg ttcgcggcgc gaaggggagc
     2701 cgtcggcggc tggcgcgggg gcgggggcgt tgtcggtgat gtgggcgtct gtcatcgcgt
     2761 gtcagtggcg agccgagccc ggctccgcca tcttcctgtg ttgttcggcc agcatgctgt
     2821 cgggcacgac gttggtcatc gcggccgaca ccttgttctt ccagcccgcc accacgtggt
     2881 cctggccctt tttcatcgcg tgccagcctt cctcggcgac ctccgcggga gaggccttct
     2941 cctccgaacc gaccttggtg tcgagcatgt cggcgcgttc gaagaagtgc gtctcggtgg
     3001 cgccgggcat caggcaggtg acgctgatgc cggtgtcctt gagctcgttg cgcagcgcga
     3061 acgagaacga gtcgatgaag gccttggtgc cgttgtagac ggcctggtac gagccgggca
     3121 tcaggccggc gatcgagccc gtgatcagga tgcgcccgcg gccgcgctgg cgcatgccgg
     3181 tgcccacgcg gtgcagcagg tccagcgtgc cggtgatgtt ggtgtcgacc acgtgcttgg
     3241 cctcgttgaa cggcttgtcg aggaagccgt ggcccaggcc gtgcccggcg ttggcgacga
     3301 gcacgtcgac gggccggccg ccgacggcca gcatcagctt gtcgacgccg aaggacgtgg
     3361 acaggtcggc ctcgacggcc tcgacgttcg cgccgagcgc gcggaaggcg gcggccgcgt
     3421 catcgagcgg gccctggtcg gccgcgatca cgaggtcgtg cccgtcctgg gccgcgagcc
     3481 gcgcgagctc ccggccgatg cccgaggacg cgccggtgac gacggcaaga gggcgttcgg
     3541 gattgagtga tgccatggcg ttctccggcg gtgtcagtgg acggtggtga ggacgacctt
     3601 gcggcagtcc tgctgcttct tctcgaagat ctcgtagccg tgcgcggcct gctccagcgg
     3661 cagcgtgtgg ctgatgatcg cctcgggatg gagatcgccc cgggcgatgt gctccagcaa
     3721 ctgcggcatg tagttctgcg cgtgcgtctg cccgcccttg atcgacaggc ccttctcgaa
     3781 cacgtcgccg aacaggaagc cgtggatcca gccggcatac acgcccggca cgctgatcgc
     3841 gccgccgcgc cgcgtcgccg cgatggcctg gcggatggac gcgccgctgg agccttcgag
     3901 cttgatggcg gtcagcgccg tctccaccgc gctgcccttg gcctcgaagc cgacggcgtc
     3961 gatcgaggcg tccacgccgc ggccgtccat gcggtcgatg atctcgttgg ccgcgtcgtc
     4021 cacctcgtcg aagttcagcg gctcgacgcc gtaggcgcgg cgcgcgaact cgagccggta
     4081 ctggtggtgg tcgaccatga agatgcgctc ggcgcccagc agccgcgccg aggcggccgc
     4141 catcaggccg accggcccgg cgccgaagat cgccaccgac gaccccttgc cgaccgccgc
     4201 gttgaccgcg gccatgtacc cggtgggcag gatgtccgag aggaacagca cctgcttgtc
     4261 ctcgaggccg gcgtcgggga tgaccagcgg gccgacgttg gccttgggca cgcgcacgaa
     4321 ctcggcctgg ccgccgggga tgccgccata caggtggctg tatccgaaca gcgccgcgcc
     4381 ggcgcgcgtg tccttcttgt tgatgatggc gccgcggtcg gggttggtcg tctcgcacgc
     4441 ggcgaacagc ttcctgttgc agaagaagca ttgcccgcac gagatggtga acggaatgac
     4501 gacgcggtcg ccccgcttga ccctggtcac cgccggcccg gtctcctcga cgatgcccat
     4561 gaactcgtgg ccgaggatgt cgccgctctc catcatcggg atctttccgc gatagatgtg
     4621 caggtcggag ccgcagatgg cggtggcggt cacgcgcagc aggatgtcgt cgggctcgac
     4681 gagccggggg tcgggaaccg tctcgacccg gacatccttg gagccgtggt aggtgagagc
     4741 tttcatggaa tctccggtca tggaagaagc ggggcgcggg gcgcttggac cggaagttcg
     4801 gcaatgcccg ttccccgtcc gtgacttccc gcccgacgcg gcacagcagt tgctcggcca
     4861 cgcgaccccc cgacaacgag ccgcacgatg cccatgtccc cctcgccgcg accgcggccc
     4921 cagcgcacgg gcatcaccga gcgcgaccgc ggccacgaca ccgcgctcgt gatcctggac
     4981 atgatcagct gttggtcctt cccggatgcg gacgcgctgg tgcgcgctgc ccacgaggtg
     5041 gcgccg
//
LOCUS       DATCRO010000909         4469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1056432, whole genome shotgun sequence.
ACCESSION   DATCRO010000909 DATCRO010000000
VERSION     DATCRO010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056432"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VIN75_24700"
     CDS             <1..486
                     /locus_tag="VIN75_24700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8977437.1"
                     /translation="ESLFIDASPKALRNLETLRQMGIRVALDDFGTGYSSLAYLRQFP
                     FDTLKIDRAFVRELVTQHDARAIVRSIVELAAALGMSTVAEGVEEPAQYELLRRAGCA
                     GVQGFLIARPMVIGQVVEMLDSWTMQRAPKSDSVPDSIFAPLDETVPGDLRAMDGGMV
                     Q"
     gene            877..2208
                     /locus_tag="VIN75_24705"
     CDS             877..2208
                     /locus_tag="VIN75_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977438.1"
                     /translation="MQDKSKTGQALIGDRVMAAALALGALASLAIANEFGQMGLAAIG
                     AGVISAIGLGVWMARPGSLLSRLVFAFALTAMVALHIDLGRGTIEFHFGVFVVLGLLL
                     VYSDWRPILLAAGLVAVEHLVTDRMQAFGADVYCTTSPSVLKVVMHAVYVILQTGAEI
                     YMTVWMNRAARQGAASAAALTDTLERLRAALATTQESAARIESASHEIAGGNADLRQR
                     TEHSAERLQRAAASMAQLTATVNGSAESAAGANRLAACASQVAERGGEVVREVVSKMD
                     DITASSRRIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRS
                     ATAAREIKSLINASVENVDAGSRLVGDAGRTMQEIVSGIRDVAGIIDEISRSATQQKA
                     GIVAMHETVADLDEMTRRNAALVQQSAVAAESLHVQAGRLTEVVSSFQLER"
     gene            complement(2233..4272)
                     /locus_tag="VIN75_24710"
     CDS             complement(2233..4272)
                     /locus_tag="VIN75_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HEY8977439.1"
                     /translation="MSLRRHALAALLPMLAATAAAASPDLPAPWLPGEPHEAHCRDAV
                     DAAGKAGPDAELQDAALRFAFTTRGGALRPLAFDNGFTRAPRPLRGELFTVTMRDKRR
                     IPASQFEIVHVDRCRHVDPTPGAVRAAERLAGESVVVQLRHPATGLRATWRAVLRGGT
                     NYVREEVRLESDTDLDIAQVALLDVELDKAWTAGTTTGSPVVADDRFFGFEHPMAETR
                     IDGTNALQFVRRKLPLRAGVAADYSAVLGVAPPGQLRRAFMAYVENERAAPFRTFLHY
                     NSWYDIGYFTPYSEQEAVDVIDTYRRKLVEARGVRMDSFLFDDGWDDHQHLWQFNEHF
                     PHGFASVRAAAARAGAAPGVWLSPWGGYGPPRQERLAAAKAAGLEVDDQGLALSGPKY
                     YALFHDATLRLLKDGGINQFKLDGTGSPDKVTPGSAFDSDFAAAISLIGDLRAVKPDL
                     FINLTTGTWPSPFWLRTADSIWRGGEDHEFAGVGSNRQRWITYRDADTYGGIVRLGPL
                     YPLNSLMLHGIVYARQARGLNVDPFDDFASEVRSYFGSGTGLQELYVSPDLLTERNWD
                     DIAAAAKWARANAATLRDSHWIGGDPARLQVYGWAAWRPGHAIVTLRNPSDKAQDFAL
                     DVGPALELPPEADRRWVARAAWDRAAAPRTLRAGQAQVIALAPFQVLVFELAPAR"
BASE COUNT          650 a         1540 c         1635 g          644 t
ORIGIN      
        1 gagtcgctgt tcatcgacgc gtcgcccaag gcgctgcgca acctcgagac gctgcgccag
       61 atgggcatcc gcgtcgcgct cgacgacttc ggcacgggct actcgtcgct cgcctacctg
      121 cgccagttcc cgttcgatac gctcaagatc gaccgcgcct tcgtgcgcga gctcgtcacg
      181 cagcacgacg cgcgcgccat cgtgcgcagc atcgtcgaac tggcggcagc gttgggcatg
      241 agcactgtgg ccgagggcgt ggaggaaccc gcgcagtacg aactgctgcg gcgcgcgggc
      301 tgcgccggcg tgcagggctt cctcatcgcg cggccgatgg tgatcgggca ggtcgtcgag
      361 atgctggaca gctggacgat gcagcgcgcg ccgaagtccg attcggtgcc cgattcgatc
      421 ttcgctccgc ttgacgagac ggtgccgggc gacttgaggg cgatggacgg cggcatggtg
      481 cagtgagaca ccattcttca gcgtcgacga ctttacccgg aggctcgcaa ctcggattgc
      541 gacgccacca ccgtgcgaat gacgggcctt cgtgccgccg ctcgttctcg tgatggacga
      601 aggggcgcgc ggccccgatg gggtcaggtc ttgaatgttg cgcgttcggc cgcctcgccg
      661 tcgccaagca tcattcgcgc aacattcaag acctgacccc atcttcgacc ccatcttcga
      721 ccccatcttc gacgatgccg gcacgcaacg attcaagacc tgaccccgca acattcaaga
      781 cctgaccccg cgatgcaggc tgtcctcaat atggacgcgt gccggccgat agcctggact
      841 gcacgtccat acaacgttgt caagggagaa cggaccatgc aggacaaatc gaagaccggc
      901 caggcattga tcggtgatcg cgtgatggcc gcggccctcg cgctgggcgc gctggcgtcg
      961 ctggcgatcg ccaatgaatt cggccagatg gggttggcgg cgatcggcgc gggcgtgatc
     1021 agtgccatcg gcctcggcgt ctggatggca cgcccgggct cgctgctgtc gcgcctcgta
     1081 ttcgcgttcg cgctgacggc catggtggcg ctgcacatcg acctgggacg cggcacgatc
     1141 gagttccact tcggcgtgtt cgtcgtgctg ggcctgctgc tggtgtattc cgactggcgc
     1201 ccgatcctgc tggccgccgg cctcgtggcc gtcgagcacc tcgtcaccga ccgcatgcag
     1261 gcgttcggcg ccgacgtcta ctgcacgacc agtccgtcgg tgctcaaggt ggtgatgcac
     1321 gcggtctacg tgatcctgca gaccggcgcc gagatctaca tgaccgtctg gatgaaccgc
     1381 gcggcgcgcc agggcgcggc gtcggccgcc gcgctgaccg acacgctcga gcgcctgcgc
     1441 gcggcgctgg cgaccacgca ggagagcgcc gcgcgcatcg agtcggcgtc gcacgagatc
     1501 gccggcggca acgccgacct gcgccagcgc acggagcatt cggccgagcg gctgcagcgc
     1561 gccgcggcct cgatggcgca actcaccgcc accgtcaacg gcagcgccga atcggccgcc
     1621 ggcgccaacc gcctggccgc ctgcgcgtcg caggtggccg agcgcggcgg cgaggtggtg
     1681 cgcgaggtcg tctcgaagat ggacgacatc acggcgtcca gccgcaggat cgccgacatc
     1741 atcggcgtca tcgacggcat cgcgttccag accaacatcc tcgcgctgaa cgcggcggtc
     1801 gaggccgcgc gcgccggcga gcagggccgc ggcttcgccg tcgtcgcgag cgaggtgcgc
     1861 tcgctggcgc agcgcagcgc gaccgccgcc cgcgagatca agagcctgat caacgcgagc
     1921 gtcgagaacg tcgacgccgg atcccgcctg gtcggcgacg ccggccgcac gatgcaggag
     1981 atcgtctcgg gcatccgcga cgtggccggc atcatcgacg agatctcgcg ctcggcgacg
     2041 cagcagaagg ccggcatcgt ggccatgcac gagacggtcg cggacctcga cgagatgacc
     2101 cggcgcaacg ccgcgctcgt gcagcagagc gccgtggccg cggagagcct gcacgtgcag
     2161 gccggccgcc tgacggaagt cgtgtcgtcg ttccagctcg agcgctgagc gtggcgccgc
     2221 cgcgccgcgt gctcagcgcg cgggcgccag ctcgaagacg agcacctgga acggtgcgag
     2281 ggcgatgacc tgcgcctggc cggcgcgcag cgtgcgcggc gccgccgcgc gatcccaggc
     2341 cgccctggcg acccaccggc gatcggcctc gggcggcagc tccagcgcgg ggccgacgtc
     2401 cagcgcgaag tcctgcgcct tgtccgacgg attgcgcagc gtcacgatcg cgtgccccgg
     2461 ccgccacgcg gcccagccgt agacctgcag ccgcgccgga tcgcccccga tccagtggct
     2521 gtcgcgcagc gtcgcggcgt tggcgcgggc ccacttcgcc gcggcggcga tgtcgtccca
     2581 gttgcgctcg gtcagcaggt cgggcgagac gtagagctcc tgcaggccgg tgcccgagcc
     2641 gaagtacgag cggacctcgg aggcgaagtc gtcgaacgga tcgacgttca ggccgcgcgc
     2701 ctggcgggcg tagacgatgc cgtgcagcat cagcgagttc agcggataca gcggcccgag
     2761 gcgcacgatg cccccatagg tatcggcgtc gcggtaggtg atccagcgct ggcggttgct
     2821 gccgacgccg gcgaactcgt gatcctcgcc gccgcgccag atcgagtcgg cggtgcgcag
     2881 ccagaacggc gatggccagg tgccggtggt caggttgatg aacaggtcgg gcttgacggc
     2941 gcgcaggtcg ccgatcagcg agatggccgc ggcgaagtcg ctgtcgaagg cgctgccggg
     3001 cgtcaccttg tcggggctgc cggtgccgtc cagcttgaac tggttgatgc cgccgtcctt
     3061 gagcagccgc aacgtcgcgt cgtggaacag ggcgtagtac ttcggcccgg acagcgcgag
     3121 gccctggtcg tccacctcga ggccggcggc cttggcggcc gcgaggcgtt cctggcgcgg
     3181 cggcccgtag ccgccccagg gcgacagcca cacgccgggc gcggcgcccg cgcgcgccgc
     3241 ggccgcgcgc acggacgcga agccgtgcgg gaagtgctcg ttgaactgcc acaggtgctg
     3301 gtggtcgtcc cacccgtcgt cgaacaggaa cgagtccatc ctgacgccgc gcgcctcgac
     3361 cagcttgcgg cggtacgtgt cgatgacgtc gaccgcctcc tgctcgctgt acggcgtgaa
     3421 gtagccgatg tcgtaccagg agttgtagtg caggaacgtg cggaacggcg cggcgcgctc
     3481 gttttcgacg taggccatga aggcgcgccg cagctggccc gggggcgcga cgccgaggac
     3541 ggcggagtag tcggccgcga cgcccgcgcg cagcggcagc ttgcggcgca cgaattgcag
     3601 cgcgttcgtg ccgtcgatgc gcgtctcggc catcgggtgc tcgaagccga agaagcggtc
     3661 gtccgcgacc accggcgaac ccgtggtggt gccggccgtc caggccttgt cgagctccac
     3721 gtccagcagc gcgacctgcg cgatgtcgag atccgtgtcg gattccaggc gcacttcctc
     3781 gcggacgtag ttggtgcccc cgcgcaggac ggcccgccac gtggcgcgca gcccggtggc
     3841 cgggtggcgc aactggacga cgaccgactc gccggcgagc cgttcggccg cgcgcacggc
     3901 gcccggcgtg gggtcgacgt ggcggcagcg gtcgacgtgc acgatctcga actggctggc
     3961 ggggatgcgc cgcttgtcgc gcatcgtcac ggtgaacagt tcgccgcgca gcgggcgcgg
     4021 cgcgcgcgtg aacccgttgt cgaacgccag gggtcgcagc gcgccgccgc gcgtggtgaa
     4081 cgcgaagcgc agggcggcgt cctgcagttc ggcgtccggg ccggccttgc ccgccgcgtc
     4141 gacggcgtca cggcagtgcg cctcgtgcgg ttcgcccggc agccagggcg cgggcaggtc
     4201 gggcgacgcg gctgccgcgg tggcggcgag catcggcagc agggcggcga gcgcgtggcg
     4261 gcgaagggac atggcggctc cgttgacgtc ggggtcgtgc cattgtccgc agctggcggc
     4321 gcgagaggcg ggcgttcctc gggccggctg ccgaacaaat gccggcgcgt gcaaagccag
     4381 aagggtctgg cctctcctca tgtcgccctc ggattgcggc catcgacaac gacgccgatg
     4441 gcgacttgag gcgaaggcct gacccggag
//
LOCUS       DATCRO010000910        11632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_201661, whole genome shotgun sequence.
ACCESSION   DATCRO010000910 DATCRO010000000
VERSION     DATCRO010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201661"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VIN75_24715"
     CDS             <1..585
                     /locus_tag="VIN75_24715"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEY8977440.1"
                     /translation="VSMCTANADPAWIRHYCAESYYEVDPRLAQATRSSLPAAWTLQQ
                     LEAAAVDTPGRAPLRRFVADLAATGMRGGSFLMLPGQAEGRRHFVSLLARDPDRRLLS
                     GETIGQVLTIGFCLHEYYTRYSGLPEPEPAPLTAVQREILGHVARGESDKRIAHHLRI
                     SSHAVDYHMRQLRSRFAVHNRVQLAQASRLMEGA"
     gene            729..1394
                     /locus_tag="VIN75_24720"
     CDS             729..1394
                     /locus_tag="VIN75_24720"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8977441.1"
                     /translation="MDDATDDDSDGGGAAAARALAPPGEVTRLLASWSGGDDEALARM
                     VPMVYEDLRRMARRHLQREREGHTLQGTGLVHEVYLRLADKRPTQWASRAHFFSWAST
                     MMRHILIDHARSRHADKRGGGIEVLSLDALREDRGTLPGLSAAVPDDTPDLIAVDQAL
                     RKLERIDPQQGRVVELRYFCGLSVAETAVALEVSPATVKRDWVTARAWLLRELGRAKA
                     GSA"
     gene            complement(1418..9733)
                     /locus_tag="VIN75_24725"
     CDS             complement(1418..9733)
                     /locus_tag="VIN75_24725"
                     /inference="COORDINATES: protein motif:HMM:NF027340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4781 domain-containing protein"
                     /protein_id="HEY8977442.1"
                     /translation="MSTPGLTASGSSIPFMPLLFNPNNDQAAQAAAQARALAAAQAAA
                     AAAEAAARAAQQAAAAAAKKAAASRQAADEADARKKASASDLAQKKATLEDAKRDDLK
                     AGRDPATHPSAATVKAQADYDAAGQQDAIVNPPAGADDPLATAQADTAARFRTFVGAT
                     ESGDQAQAKAAQGAWLDAVQHQMQVAALEAGAKGQDQKAQVQAQLDGVLKAVDKDGTF
                     DSGTLRKALQPATDQIGATPPDQVRAGMAYQSENDAGQARIAALQGQVKDLQAKADAA
                     DAAAAPPKVYGPYVPGHAPSQPSFTTASGNGSPVLATTAQQEAAAAHDALDAAKDKLA
                     SLEAVYGHTDPSNGRNDTIGTLRADYDADCATSAAAQAKAAWLKLAGAPGTSAKDLSA
                     AYDAWQRAVDRQMLALKLQDATHASAALLAATQDRTAAEAALKKQPTSPFFATTTTSF
                     GQTGLLVSDDAPAGAQTPQQRVASANAAFAAATRKSQDAQAALDQAWTDQKGGPQGMG
                     LDISGTIDHADDLRAAVDKAQTDLSAAQSEFDAAQFGRPGALTLDDARTRLGLAQQAV
                     DLAQAKVKVLDAMQALRGAQMATDGGAPPANLDTLEANVRDAQKAVDTLQGTRLVTAD
                     DRKTLTTTTLPAEAKTLVGIDAQIKAARDKGDTGSKDLRSLLDQRDWLEDKITLQKSA
                     IALEDAQDAASAASYEYGRSTRPSGVDLTATGHGDEADAALTWGITPGGPGGQPRISG
                     LPSNIKASDVRIEKDGDTWVAVFDKDSGAYAVRWTGGRFPVRTYADGSKQGDIAIEKG
                     HKFKLDPATARLWEATNTDPAVGRSAIVVAKDNFDKALAQFKLDPQTDDAGKPVLGPD
                     GRLSTAMTTDVAGQSVAAIDFNTDQTAAKTAADANVAMLDKALAAANAAASAAPADAT
                     LKAAQARAQSDYDLARSNDTAIGAVLDWQHARLARQIEDADTRDGRAGLMCYATPPRQ
                     TQDRLYQVAVSKVTDWRSLQQKVAVGGAQHDVDVAQAAFDQWHGAHSYLSAESVDASP
                     QYQALQAAHGRLDVARRDLTLAATTDADARQKAYIAQNLAPGKQGDPRSLYQLFNQDP
                     QVMAQSIINQDYIRNGSVAQAYAGRDAIGKMVSGELLTNADATRTIVDQIVAVGGDDA
                     KVTIIPVVYAMNADADQGGGIVKTALFKVQDGSDPNSFKFVDAYGERYDSVDDYRANN
                     NLPVDGVKLAMPKDGNITLDADGNVQLFTGDARTETGWQHFTRVTDFNAVVGVVGLVA
                     GVVMEVGSAGILTEIAAPLAIASTAYLAGTSAQDLANRSQHGLSIDPFTNREAGLDWL
                     NLGASALALPGLGSAARVSGETAELTRAGAQADSIAVVTSKGRALLRNGTDVTDHAED
                     YHLIAGSTTMTNATRTTAGVMGGAAMADQTIDVAQNWSTMTSAERQEQGEGLLLNVGM
                     MLGAHAAGRIAGARAARGVRQEPSLATETTTLATDASAPPGHDDATGLGTATPDMAID
                     AARVGYDAPEPEEGLVGPGWYLQTMKRAAMDPAVRAKIAERLAEHNGVTPEEAAPQVE
                     QMFGTGADVDVAFDTPALQPIYLGLVRDALGELEHDGHRVALDGYAPANAPWADTYAI
                     RPSRPPQLRTAADLASALENTTRHAGRIAAAIKAGDMKVELLDHDTYVTTHAAKDRDS
                     DSPLAFTQGHTIYLDLGRGGAGERGWALLLDAVHEGTHALDNLSGYDVDRPYAIRYKG
                     RTLATGTHAPGVFHAGDQEARAYFHQLEFARALGLSTETFGEAEFPATLPEGTFGAGD
                     GMEDIHAHIQMRYPNALKNRARLSAAGMDQPPIEYQHGSTTRGAAPTPPRAPAFKAAR
                     MRLPEHAKVDIDTMDWSTRALQADAESTAGTPPPLALAHEADAAIAGALRSDPAAGSK
                     DFFVVSRRGPGRAIADGLGVDTSKRYATFDEAVAAVGRNRNQRDGAWVHRVGGGNVDP
                     ARLAKGKGAHLDAGDIAGSIFVDGQRQPTALVVANPAAAAWTELAPAGSAARPGGALV
                     QPGAGVRTLADADAVVAHADELVAQYRPGSGRMAPKGSAPLVDALRQSRLTGARTRFV
                     FSDKPPSEVFADGTTLHGQPGFRSFGKASAAAQEGGGGWVYRAELPQLRSKGQPVQWN
                     VVGGLHVYDDGSVMPVGVARADAAAWTRPGALRVAGTAATGVLAFAGAAWFARELPLN
                     VVLPFGLPHDTLTLAAVGTMVRSAAKVSRFGHAGRWQSRFADLQDTAGRRADFESALR
                     QLDALDGRPGTRKLSQAVADFRAADRALSVTKTKARGAAIRAALGPLREAAGAPGDTL
                     MATLSGHVDAKAAFDAALDRHATFLSRITDTNGSARARRFVAGMHDNVDDQGTPTGPV
                     TRFRQQFGDVQGALVAWERDGPAALPDDALKAGLKEAVDQMHVAPELRKGATPVRRVL
                     DGVQFTTYAIAWGASLHGVSGETLSQAASWGFNAAFAADAVRIAGVRFAGLLDRKLSD
                     GATRTASSKLYTQWLPGFDDTLTTAAGLATLGNGVQDLMGLHMGAKGLVARHAFTWVH
                     DLRVGVTGAYAGVNGVNWGLSASRRFGDPAAGDARGRRVNFWLKVGTGAAAAVLVADG
                     ILQKAVASNSPQNANDPNGNDRNHKPGGQPTPGPSPSSTPGLPVPSGSATATPTTRPG
                     PSTSGKPPTGPSTSPAAPRPTHAQVIVAAVDPRRDTLWGIARDNEDTLLTTGRSAAVR
                     AAGGGRDAQTLVALDQLFQLDPQRGFRPERMEGVASAQAVDPDTWQPSWRIDVHNPAV
                     S"
     gene            complement(9759..10793)
                     /locus_tag="VIN75_24730"
     CDS             complement(9759..10793)
                     /locus_tag="VIN75_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY8977443.1"
                     /translation="MSANKDQKPTPRRLREARKRGDVVFSSDVSSTTVFAMVVAALWL
                     LGATSFGLLRELWLHATSAQLLARPDDRLPELLRHTVRVLLWIGLPITGLAALAGIAG
                     SFFQVGGMLAFSRLKPDVSRLSPSRGLQRIFSTRNLVNLLKMLVKTLLLAVLVAATLR
                     GLLGTALKLGWRAPDAIMAVGAHALLGLFAAATAIYAALAAVDYVHQHHEHIKSLRMS
                     IDEVRRDHKDAEGDPLTRARRRGAHLEAIYAGLGDRVAAASAVIHSGRVAVALQYLGE
                     RDLPRVIARGENEVAQRLRELAADARVPLAEDAGLAERLYADVPLDHPIPRALYASVA
                     RLLRWAQGDA"
     gene            complement(10790..>11632)
                     /gene="sctT"
                     /locus_tag="VIN75_24735"
     CDS             complement(10790..>11632)
                     /gene="sctT"
                     /locus_tag="VIN75_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEY8977444.1"
                     /translation="PPRNWPPPAGPRGAPTGADAMDMPPELAALQSWFTPVALTLPRL
                     LAVFAIAPFFSGNLMSGLVRNGVVLLLAIFMSPAASDLPVLTLGGWVLVAVKEAFIGV
                     LLGLGFGIFIWAVQSVGDLVDFQTGSGSAPFFDPVGGHDGGPTGQFLGWVVITLFMSA
                     GGMLAMVRVIVDSFRLWPVASFYPHVGVVIEQFAVRQGDTLFLWAVKLAAPVIFVLLL
                     VELGIGLVGRVAPQLNIFAFAQPVKSVLANLMLLLFMYFLYDSLREFLKPENGVLDFL
                     RNAL"
BASE COUNT         1426 a         4261 c         4221 g         1724 t
ORIGIN      
        1 gtctcgatgt gcacggccaa cgccgacccg gcatggatcc gccactactg cgccgagagc
       61 tactacgagg tcgacccgcg cctggcacag gcgacgcgct cgagcctgcc ggcggcctgg
      121 acgctccagc agctggaggc cgccgccgtg gacacgccgg ggcgcgcgcc gctgcgccgc
      181 ttcgtggccg acctcgccgc gacgggcatg cgcgggggca gcttcctcat gctgcctggc
      241 caggccgagg gccggcggca cttcgtcagc ctgcttgccc gcgaccccga ccggcgcctg
      301 ctgtcgggcg agacgatcgg ccaggtgctc acgatcggct tctgcctgca cgagtactac
      361 acccgctaca gcggcctgcc cgaacccgag cccgccccgc tgacggccgt gcagcgcgag
      421 atcctgggcc atgtcgcgcg cggcgaaagc gacaagcgca tcgcgcacca cctgcggatc
      481 tcctcgcacg ccgtcgacta ccacatgcgc cagctgcgca gccgcttcgc cgtgcacaac
      541 cgcgtgcagc tggcgcaggc ctcgcggctg atggaaggcg cctgagccgc acgtccccgc
      601 gcgatacgcg tggatacccc ttgggggcat gcgcgagcgc cggccagccg ccataatccg
      661 ggccgcacac cgcgcggctg gaaagcccgc gccacggtgc agattcaact cgagcacggg
      721 cggcccggat ggacgacgcg acggacgacg acagcgacgg cggcggcgcc gcggcggcgc
      781 gcgcgctcgc gccccccggc gaggtcacgc gcctgctggc aagctggtcg ggcggcgacg
      841 acgaggcgct ggcgcgcatg gtgcccatgg tctacgagga cctgcgacgc atggcccggc
      901 gccacctgca gcgcgagcgc gagggccaca cgctgcaggg cacgggcctc gtgcacgagg
      961 tctacctgcg cctggccgac aaacggccga cgcaatgggc atcgcgcgcg catttcttct
     1021 cgtgggcgtc gacgatgatg cggcacatcc tgatcgatca tgcgcgctcg cgacacgctg
     1081 acaagcgcgg cggcggcatc gaggtgctga gcctggacgc gctgcgcgag gatcggggca
     1141 cgctgccggg gctgtccgcc gcggtgcccg acgacacgcc cgacctgatc gccgtcgacc
     1201 aggcgctgcg caagctcgag cgcatcgacc cgcagcaggg ccgcgtggtc gagctgcgct
     1261 acttctgcgg cctgagcgtc gcggagacgg cggtggcgct cgaggtgtcg cccgccaccg
     1321 tcaagcgcga ttgggtcacc gcgcgcgcct ggctgctgcg tgagctgggc cgtgccaagg
     1381 ccggctcggc ctgagcggcg gcgtccgcgt cgcccggtca tgacaccgcc gggttgtgga
     1441 catcgatgcg ccagctgggt tgccacgtgt ccggatcgac cgcctgcgcg ctcgcgacgc
     1501 cttccatccg ctccggcctg aagccgcgct gcgggtcgag ctggaacagc tggtccagcg
     1561 ccacgagcgt ctgcgcgtcg cggccgccgc cggccgcacg gacggcggcg gaccggccgg
     1621 tcgtcagcag ggtgtcctcg ttgtcgcggg cgatacccca cagggtgtct cgccgcggat
     1681 cgacagcggc cacgatcacc tgggcgtggg tcgggcgcgg cgcggccggg ctcgtcgagg
     1741 gcccggtggg cggcttgcct gaggtcgagg gcccgggccg ggtcgtcggc gttgccgtcg
     1801 ccgagccgct cggcacgggc aggccgggcg tggacgatgg cgacggcccc ggcgtcggct
     1861 ggccgcccgg cttgtggttc ctgtcgttgc cgttcgggtc gttggcgttc tgcggcgagt
     1921 tcgacgcgac ggccttctgc aggatcccgt cggcgacgag cacggccgcc gccgcgccgg
     1981 tgccgacctt gagccagaag ttcacgcgtc gcccgcgcgc gtcgccggcc gccggatcgc
     2041 cgaagcggcg gctcgcggac aggccccagt tcacgccgtt gacgcccgcg taggcgcccg
     2101 tcacgcccac gcgcaggtcg tggacccagg tgaaggcatg cctggcgacc aggcccttgg
     2161 cgcccatgtg caggcccatc aggtcctgca cgccgttgcc cagcgtcgcc aggcccgcgg
     2221 ccgtggtcag cgtgtcgtcg aagccgggca accactgcgt gtagagcttg ctcgacgcgg
     2281 tccgggtcgc accgtccgac agcttgcgat cgagcaggcc ggcgaagcgc acgcccgcga
     2341 tgcgcaccgc gtccgctgcg aacgccgcgt tgaagcccca gctcgcggcc tggctcagtg
     2401 tctcgccgct gacgccgtgc aggctggcgc cccaggcgat cgcgtaggtc gtgaactgca
     2461 cgccatcgag cacgcggcgc accggcgtcg cgcccttgcg cagctcgggg gccacgtgca
     2521 tctggtcgac cgcctccttc agccccgcct tcagcgcgtc gtcaggcagg gccgcgggtc
     2581 cgtcgcgctc ccatgcgacg agcgcgccct gcacgtcgcc gaactgctgg cggaacctgg
     2641 tgacggggcc ggtgggcgtg ccctggtcgt cgacgttgtc gtgcatgccc gccacgaagc
     2701 ggcgcgcgcg cgcgctgccg ttcgtgtcgg tgatcctgga caggaacgtg gcgtgacgat
     2761 cgagcgccgc gtcgaaggcc gccttcgcgt cgacgtggcc ggacagcgtg gccatcagcg
     2821 tgtcgcccgg cgcgcccgcg gcctcgcgca gcggccccag cgccgcgcgg atcgccgcgc
     2881 cgcgcgcctt ggtcttcgtg accgacagcg cgcgatcggc agcgcggaaa tcggcgaccg
     2941 cctgcgacag cttgcgcgtg ccgggccggc cgtccagcgc gtcgagctgg cgcaaggccg
     3001 attcgaagtc ggcgcgcctg ccggccgtgt cctgcaggtc ggcaaagcgc gactgccagc
     3061 gcccggcgtg gccgaagcgc gagaccttgg ccgccgagcg caccatcgtg ccgaccgccg
     3121 ccagcgtcaa cgtgtcgtgc ggcagaccga agggcaggac cacgttcagg ggcagttccc
     3181 gcgcaaacca ggcggcgccg gcgaatgcga gcacgcccgt cgctgcggtg cccgcgacgc
     3241 gcagcgcgcc cggacgcgtc cacgcggccg cgtccgcgcg cgcgacgccc accggcatca
     3301 cgctgccgtc gtcgtagacg tgcaggccgc ccaccacgtt ccactgcacg ggctggccct
     3361 tggaccgcag ctgcggcagc tcggcgcgat agacccagcc gccgccgccc tcctgcgccg
     3421 cggcgctcgc cttcccgaac gaccggaagc cgggttggcc gtgcagcgtc gtcccgtcgg
     3481 cgaagacctc ggacggcggc ttgtccgaga acacgaaacg cgtgcgcgcg ccggtcagcc
     3541 gcgactggcg cagcgcatcc acgaggggtg cgctgccctt gggcgccatg cggccgctgc
     3601 cggggcgata ttgcgcgacg agctcgtccg cgtgcgcgac gaccgcgtcg gcatcggcca
     3661 gcgtgcgcac gccggcgccc ggctgcacca gcgcgccgcc gggccgcgcc gcgctgccgg
     3721 ccggcgcgag ctccgtccac gccgcggcgg ccgggttcgc gacgacgagc gcggtcggct
     3781 ggcgctggcc gtcgacgaag atcgagccgg cgatgtcgcc cgcgtccagg tgggcgccct
     3841 tgcccttggc gagccgcgcc ggatcgacgt tgccgccgcc gacgcggtgc acccatgcgc
     3901 cgtcgcgctg gttgcggttc cggccgaccg cggccaccgc ctcgtcgaag gtggcgtagc
     3961 gcttgctggt gtccaccccg agtccgtcgg cgatcgcgcg gccgggcccg cggcgcgaga
     4021 cgacgaagaa gtctttcgag cccgccgcgg ggtcggaccg cagcgcgccg gcgatcgcgg
     4081 cgtcggcctc gtgggccagg gccagcggcg gcggcgtccc ggccgtcgat tcggcatcgg
     4141 cctgcagcgc gcgcgtcgac cagtccatcg tgtcgatgtc caccttggcg tgctcgggca
     4201 ggcgcatcct ggcggccttg aatgccggcg cacgcggtgg cgtcggggcg gcgccgcgcg
     4261 tggtcgagcc atgctggtac tcgatcggag gctgatccat gccggcggcc gacaggcgtg
     4321 cgcggttctt cagcgcgttc ggatagcgca tctggatgtg cgcgtggatg tcctccatgc
     4381 cgtcgccggc cccgaaggtg ccctcgggca gcgtcgcggg aaattccgct tcgccgaagg
     4441 tctcggtgct caagcccagc gcccgtgcga actcgagctg gtggaagtac gcgcgcgcct
     4501 cctggtcgcc ggcgtggaag acgcccggcg catgcgtgcc ggtcgccagc gtccgcccct
     4561 tgtaccggat ggcgtagggg cggtcgacgt cgtagccgct gagattgtcc agggcgtgcg
     4621 tgccctcgtg cacggcatcg agcagcaggg cccagccccg ctcgccggcg ccgccccggc
     4681 cgaggtcgag gtagatggtg tgcccctgcg tgaacgccag cgggctgtca ctgtcccggt
     4741 ccttcgctgc gtgcgtggtc acataggtgt cgtgatccag cagttccacc ttcatgtccc
     4801 cggccttgat cgccgcggcg atgcgcccgg catggcgcgt ggtgttctcc agcgccgagg
     4861 ccaggtcggc cgccgtcctg agctgcggcg gacgcgacgg ccgtatcgcg taggtgtcgg
     4921 cccacggcgc gttggccggc gcgtagccgt ccagcgcgac ccgatggccg tcgtgctcga
     4981 gctcgcccag cgcgtcgcgc accaggccca ggtagatggg ttggagcgca ggcgtgtcga
     5041 acgcgacgtc cacgtccgcg ccggtgccga acatctgctc gacctgcggc gccgcctcct
     5101 cgggcgtcac gccgttgtgc tcggccaggc gctcggcgat cttggcgcgc accgccgggt
     5161 ccatcgcggc gcgcttcatc gtctggaggt accagccggg gcccacgagg ccctcctccg
     5221 gttccggagc gtcgtagccc acgcgagccg cgtcgatggc catgtccggg gtcgccgtgc
     5281 ccaggcccgt ggcgtcgtcg tggcccggag gcgcgctcgc gtccgtggcc agcgtcgtcg
     5341 tctccgtggc gagggacggt tcctgccgga cgccgcgcgc cgcgcgggcg ccggcgatgc
     5401 ggccggcggc gtgcgcaccc agcatcatcc ccacgttcag cagcaggccc tcgccctgct
     5461 cctggcgctc ggcgctggtc atcgtgctcc agttctgcgc cacgtcgatc gtctggtcgg
     5521 ccatggccgc gccgcccatc acgccggccg tcgtgcgcgt ggcgttggtc atggtggtcg
     5581 aacccgcgat caggtggtag tcctcggcgt ggtccgtcac gtcggtgccg ttgcgcagca
     5641 gcgcgcgtcc cttgctcgtg accaccgcga tgctgtcggc ctgggcgccg gcacgcgtga
     5701 gctcggccgt ctcgccgctg acgcgcgccg ccgatcccag gccgggcagc gcgagcgccg
     5761 aggcgccgag gttgagccag tccaggcccg cctcgcggtt cgtgaacggg tcgatgctca
     5821 ggccgtgctg cgagcggttg gccaggtcct gtgccgaggt gcccgccagg taggcggtcg
     5881 acgcgatggc cagcggcgcc gcgatctccg tgaggatgcc tgccgagccc acctccatca
     5941 cgacgcctgc caccaggccc accacgccga cgacggcgtt gaagtccgtg acgcgcgtga
     6001 agtgctgcca gcccgtctcc gtgcgcgcat cgcccgtgaa cagctgcacg ttcccatccg
     6061 cgtcgagcgt gatgttgccg tccttgggca tggccagctt gacgccgtcg acgggcaggt
     6121 tgttgttggc acggtagtcg tcgacgctgt cgtatcgctc gccgtacgca tccacgaact
     6181 tgaacgagtt cgggtcgctg ccgtcctgca ccttgaacag cgcggtcttg acgatgccgc
     6241 cgccctggtc ggcatcggcg ttcattgcat agaccaccgg gatgatggtc accttcgcgt
     6301 cgtcgccacc gacggcgacg atctggtcga cgatcgtcct ggtggcgtcc gcgttggtga
     6361 gcagctcgcc gctgaccatc ttgccgatcg cgtcgcgccc ggcataggcc tgcgcgacgc
     6421 tgccgttgcg gatgtagtcc tggttgatga tggactgcgc catcacctgc gggtcctggt
     6481 tgaacagctg gtacaacgag cgcgggtcgc cctgcttgcc gggcgcgagg ttctgggcga
     6541 tgtaggcctt ctggcgcgcg tcggcgtcgg tggtcgcggc cagcgtgaga tcgcgcctgg
     6601 ccacatcgag ccggccatgc gcggcctgga gcgcctggta ctgcggcgac gcgtcgaccg
     6661 actccgcgga caggtacgaa tgcgcgccgt gccactggtc gaacgcggcc tgcgcgacat
     6721 cgacgtcatg ctgcgcgccg cccacggcca ccttctgctg cagcgagcgc cagtcggtga
     6781 ccttggacac cgcgacctgg tacagcctgt cctgcgtctg ccgcggcggc gtcgcgtagc
     6841 acatcagacc ggcccggccg tcacgcgtgt ccgcgtcctc gatctggcgc gccaggcggg
     6901 catgctgcca gtccagcacc gcgccgatcg ccgtgtcgtt cgacctggcc aggtcgtagt
     6961 cggactgcgc cctggcctgc gcggccttca acgtcgcatc cgccggcgcg gccgacgccg
     7021 cggcgttggc ggccgccagc gccttgtcca gcatggcgac gttggcgtcc gccgcggtct
     7081 tggcggcggt ctggtcggtg ttgaagtcga tcgcggcaac ggactggccg gccacgtcgg
     7141 tcgtcatcgc cgtcgacagc cggccatccg gtccgagcac gggcttgccg gcatcgtcgg
     7201 tctgcggatc gagcttgaac tgcgccagcg ccttgtcgaa gttgtccttg gccaccacga
     7261 tggcgctgcg gcccacggcc gggtcggtgt tggtcgcctc ccacagccgc gcggtggccg
     7321 gatcgagctt gaacttgtga cccttctcga tggcgatgtc gccttgcttc gagccgtcgg
     7381 cgtacgtgcg caccgggaag cgcccgccgg tccagcgcac cgcgtaggcg cccgagtcct
     7441 tgtcgaacac ggcgacccag gtgtcgccgt ccttctcgat cctgacgtcc gacgccttga
     7501 tgttggacgg caggccgctg atcctcggct gcccgccggg gccgcctggc gtgatgcccc
     7561 aggtcagcgc cgcgtcggcc tcgtcgccgt ggcccgtggc ggtcaggtcg acgccggagg
     7621 gccgggtcga acggccgtac tcgtaggacg cggcggacgc ggcgtcctgc gcgtcctcca
     7681 atgcgatcgc gctcttctgc agcgtgatct tgtcctcgag ccagtcgcgc tggtccagca
     7741 gcgatcgcag atccttgctg ccggtgtcgc ccttgtcgcg cgcggccttg atctgcgcgt
     7801 cgatcccgac cagggtcttc gcctcggcgg gcagcgtcgt cgtcgtgagc gtcttgcggt
     7861 cgtcggccgt caccaggcgc gtgccctgca acgtgtcgac cgccttctgc gcgtcgcgca
     7921 cgttggcctc gagggtgtcc aggttcgcgg gcggcgcgcc gccgtccgtg gccatctggg
     7981 cgccgcgcaa cgcctgcatc gcgtccagca ccttgacctt ggcctgggcc agatcgaccg
     8041 cctgctgcgc caggccgaga cgcgtgcgcg cgtcatccag cgtgagcgcg ccgggccggc
     8101 cgaactgtgc cgcgtcgaac tccgactgcg cggcggacag gtcggtctgc gccttgtcca
     8161 ccgccgcacg caagtcgtcc gcgtggtcga tggtcccact gatgtccagg cccatgcctt
     8221 gcgggccgcc cttctggtcc gtccaggcct ggtcgagcgc cgcctgggcg tcctgcgact
     8281 tccgggtggc cgcggcgaac gcggcgttgg cgctggcgac ccgctgctgc ggcgtctgcg
     8341 cgccggcagg cgcgtcgtcc gacaccagca ggccggtctg accgaagctg gtcgtcgtcg
     8401 tggcgaagaa cgggctcgtc ggctgcttct tcaacgcggc ctcggccgcc gtcctgtcct
     8461 gcgtggccgc gagcagcgcg gcgctggcat gggtggcgtc ctgcagcttg agcgccagca
     8521 tctgccggtc gacagcgcgc tgccaggcgt cataggccgc ggacaggtcc ttggccgacg
     8581 tccccggcgc acccgcgagc ttcagccagg ccgccttggc ctgcgcggca gcactggtgg
     8641 cgcagtcggc gtcgtagtcg gcccgcagcg ttccgatggt gtcgttcctg ccgttggacg
     8701 gatcggtgtg gccatagacg gcctcgaggc tcgcgagctt gtccttggcc gcgtcgagcg
     8761 cgtcgtgcgc ggccgcggcc tcctgctgcg ccgtggtcgc gagcaccggg ctgccgttgc
     8821 cgctggccgt ggtgaacgac ggctgcgacg gcgcgtggcc gggcacatac ggcccgtaga
     8881 ccttgggcgg ggcggccgcg gcgtcggcgg cgtcggcctt ggcctgcaga tccttgacct
     8941 ggccctggag ggcggcgatc cgcgcctggc ccgcatcgtt ctccgactgg tacgccatgc
     9001 cggcgcggac ctggtccggc ggcgtggcgc cgatctggtc ggtcgccggc tgcagcgcct
     9061 tgcgcagcgt gccggagtcg aaggtcccgt ccttgtcgac ggccttgagc accccatcga
     9121 gctgcgcctg cacctgggcc ttctggtcct ggcccttggc gccggcctcc agcgcggcca
     9181 cctgcatctg gtgttgcacc gcgtccagcc atgcgccctg cgctgccttc gcctgggcct
     9241 ggtcgcccga ttcggtcgcg ccgacgaacg tcctgaacct ggccgcggtg tcggcctggg
     9301 cggtcgcgag cggatcatcg gcgccggcgg gcgggttgac gatcgcgtcc tgctggccgg
     9361 cggcgtcgta gtcggcctgc gccttcacgg tggctgcgga cggatgggtc gccgggtcgc
     9421 gcccggcctt caggtcgtcg cgcttggcgt cctccagcgt cgccttcttc tgcgcgaggt
     9481 cggacgccga ggccttcttc cgcgcatcgg cctcgtcggc ggcctggcgc gaggccgcgg
     9541 ccttcttcgc ggcggctgcc gcggcctgct gtgccgccct ggccgccgcc tcggccgcgg
     9601 cagcagccgc ctgcgcggcc gcgagcgcgc gcgcctgcgc ggcggcctgc gcagcctggt
     9661 cgttgttcgg gttgaacagc agcggcatga acgggatgct gctgcccgat gcggtcaggc
     9721 caggggtgga catcgcggtt cctctcgcaa gacaaggttc aggcgtcgcc ctgcgcccag
     9781 cgcagcagcc tggcgacgga cgcgtacagc gcgcgcggga tcgggtggtc gagcggcaca
     9841 tcggcataca ggcgctcggc caggccggcg tcctcggcca gcggcacgcg cgcgtcggct
     9901 gccagttcgc gcaggcgttg cgcgacctcg ttctcgccac gcgcgatgac gcgcggcagg
     9961 tcgcgctcgc ccaggtattg cagcgcgacg gccacgcgcc ccgaatggat gacggccgac
    10021 gccgccgcga cgcggtcgcc caggccggcg tagatggcct ccaggtgcgc cccgcgccgt
    10081 cgggcgcgcg tgagggggtc gccttcggcg tccttgtggt cgcgccgcac ctcgtcgatc
    10141 gacatgcgca ggctcttgat gtgctcgtgg tgctggtgca cgtagtcgac cgccgcgagc
    10201 gccgcgtaga tcgccgtggc ggcggcgaac agccccagca gcgcgtgcgc gccgacggcc
    10261 atgatcgcgt cgggcgcgcg ccagccgagc ttgagcgcgg tgcccagcag gccgcgcagc
    10321 gtcgcggcca ccagcaccgc cagcaacagc gtcttgacca gcatcttgag caggttgacg
    10381 aggttgcgcg tcgagaagat gcgctgcagg ccgcgcgagg gcgacagccg cgacacgtcg
    10441 ggtttcaggc ggctgaaggc gagcatgccg ccgacctgga agaacgagcc cgcgatgccc
    10501 gccagcgccg ccagcccggt gatcggcagc ccgatccaca gcagcacgcg gaccgtgtgg
    10561 cgcagcagct cgggcaggcg gtcgtccggt cgcgcgagca gctgcgcgct ggtggcgtgc
    10621 agccacagct cgcgcaacag cccgaagctc gtcgcgccca gcagccacag cgcggccacg
    10681 accatcgcga acaccgtggt cgacgagaca tccgagctga agacgacgtc gccgcgcttg
    10741 cgcgcctcgc gcagccggcg cggagtcggc ttctggtcct tgttcgcgct cacagggcgt
    10801 tcctcaggaa gtccagcacg ccgttctccg gcttgaggaa ctcccgcagc gagtcgtaca
    10861 ggaaatacat gaacagcagc agcatcaggt tggccagcac gctcttgacg ggctgcgcga
    10921 acgcgaagat gttgagctgt ggcgccacgc ggccgaccag gccgatgccc agttccacca
    10981 gcagcagcac gaagatcacc ggcgccgcga gcttgaccgc ccacaggaac agcgtgtcgc
    11041 cctggcgcac cgcgaactgt tcgatcacca cgcccacgtg cggatagaac gaagcgaccg
    11101 gccacaggcg gaacgagtcg acgatcacgc gcaccatcgc cagcatcccg ccggccgaca
    11161 tgaatagcgt gatgaccacc cagcccagga actggccggt gggcccgccg tcgtgcccgc
    11221 cgaccgggtc gaagaacggc gcgctgcccg agccggtctg gaagtcgacg aggtcgccca
    11281 cgctctgcac ggcccagatg aagatgccga agcccagccc cagcagcacc ccgatgaagg
    11341 cctccttgac cgccaccagc acccagccgc ccaaggtcag cacgggcagg tcgctggccg
    11401 cgggcgacat gaagatcgcc agcagcagca cgacgccgtt gcgcaccagg ccgctcatga
    11461 ggttgcccga gaagaacggg gcgatcgcga agacggccag caggcgcggc agcgtcagcg
    11521 ccaccggcgt gaaccaggac tgcagggcgg cgagctcggg cggcatgtcc atcgcgtcag
    11581 ccccggtcgg cgcgccgcgc ggcccagcgg gcggcggcca gttcctcggc gg
//
LOCUS       DATCRO010000911         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_572779, whole genome shotgun sequence.
ACCESSION   DATCRO010000911 DATCRO010000000
VERSION     DATCRO010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572779"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VIN75_24740"
     CDS             <1..667
                     /locus_tag="VIN75_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977445.1"
                     /translation="VASVPAGSGIVVNSNSLVEIFDNDVTANATANVIISSYFSTGYF
                     STKGTAVASVPAGSGVVVNSNSLVEIFDNDVADNATANVIISSYFSTGYFSTKGTQPG
                     YDPYPRGIHVHDNRFKGGGDSPDGMDLKVLKTAMFGLTGHFPDVLWDGYVDPKRADVP
                     EERLCVDATASGVLDADGPDKYKSPRLVSDSLRCKLAPLAPVTLKAAGAAAASSAASA
                     ASV"
     gene            676..1824
                     /locus_tag="VIN75_24745"
     CDS             676..1824
                     /locus_tag="VIN75_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEY8977446.1"
                     /translation="MRRRLLTATAAGLLALLASCARAPAPVVFIADGRPEHLSDWHLL
                     NVSGGRLTPNAGVLPYDLVTPLFSDYAHKLRTVWMPQGVSAKYSPDQAFDFPVGTVLS
                     KTFYYPRAGTADAVLKTYDAEPGAPAAGLDLSRVHLVETRVLVRRAEGWTVLPYVWNA
                     AQTEADLQRTGDTQTLELVDAQGHGEKFAYVVPNENQCASCHVTNLRGGTFTPLGPKA
                     RNLNRTLAYADGSENQIARWVRAGFLTGVPAVELPRAADWRDVQQPIAARARSYLDVN
                     CAHCHQNAGTASNTSLRLDALGPADWSLGICKPSVAAGKGTGDRIDDIVPGKPDESIL
                     PFRLASTEGGVMMPELGRNTVHQEGVQLIRDWIASMPPGCKTDNPTKD"
     gene            1826..4291
                     /locus_tag="VIN75_24750"
     CDS             1826..4291
                     /locus_tag="VIN75_24750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8977447.1"
                     /translation="MQRHPARRPTSLALAAVAAVLAGASAGARAQAAAPEAAASAPAG
                     DVQVIEVTAQGRKQEVQKVPISLQLVGPDEIAKSGAVNLSQVADFVPGLSVDASQPTQ
                     PSYALRGLGNGDFGIGTDAPVGVYVNGIYTGKTGGAMLNFNDVKRVEVLKGPQGTLFG
                     RNSAGGAISIVTNDPDGQRTASGLVRVGNYGTVHAEGVLNQPLGEDFSIRLSAVGENS
                     DGWATNTVDGKKSPQAHTWGTRLGARWSPSDSTSVVLTWEHEDLNERPRPVWAVATSD
                     GAGGVASWIDPVHAPLSNDVQGGVESRLFNGMNLRVEHELTDKVLLTSTTGWRHFDSV
                     NLEDNDGTANPATYLATGNFEKSTTFEQEFRLNGKSKLADWLVGATFASEHSAQTSRV
                     DTNTTTLDPVLFNVLNGEGFGLPAPPFSLLTGIGQQAGVPGFDLLGQDWKEGIQNTLS
                     SHSEALMGDVIWHVGDKTNVTTGVRFTQDTKKFSWYNPLRSAPGLDTQLAIMSPAFFQ
                     QLVDAGALDSATAAQLTQLAQGLQATNIEFTNPAWAAAPVYTSKTWHNTSPRLVIDHQ
                     LTPDTLVFGSVTRGYQAGGFNSVSTDANGGRFDPETITSYELGAKGRVPGAGISYSAS
                     LFHYLFKNLQSITLDHSTTVPVYVITVSDVEATGLDGELNWQVASPLRLYVNGEWIDQ
                     KYKSQENLAPGANDLTGQPYGTPSLSLAAGVDVSVALAGGKAAWSLQGSHIGATRCND
                     DSQAQGACATTPAFKVGQARDRFDTRLGWANDHWGAALIVTNLFDKRYVSGLSNLSAA
                     VGVPYTASVSDPRKFQIEFSAKL"
BASE COUNT          880 a         1937 c         1765 g          821 t
ORIGIN      
        1 ggtggccagc gtgccggcgg gctcgggcat cgtcgtcaac tccaactcgc tggtggagat
       61 cttcgacaac gacgtcaccg ccaacgcgac ggccaacgtc atcatctcca gctacttctc
      121 caccggctac ttcagcacca agggcacggc cgtcgccagc gtgccggcgg gctcgggggt
      181 cgtcgtcaac tcgaactcgc tggtcgagat cttcgacaac gacgtcgccg acaacgcgac
      241 ggccaacgtc atcatctcga gctacttctc caccggctac ttcagcacca agggcacgca
      301 gccgggctac gacccgtacc cgcgcggcat ccacgtgcac gacaaccgct tcaagggcgg
      361 cggcgactcg cccgacggca tggacctgaa ggtgctcaag acggcgatgt tcggcctcac
      421 gggccatttc cccgacgtgc tgtgggacgg ctacgtcgac cccaagcgcg ccgacgtgcc
      481 cgaggagcgc ctgtgcgtgg acgccaccgc gtcgggcgtg ctcgacgccg acggcccgga
      541 caagtacaag agcccgcgcc tggtcagcga ctcgctgcgc tgcaagctgg cgccgctggc
      601 gcccgtcacg ctcaaggccg ccggcgccgc ggcggcttcc tccgctgcat ccgcggcttc
      661 cgtctgaccg gatccatgcg acggcgtctc ctgaccgcga ctgccgccgg cctgctcgcg
      721 ctgctggcca gctgcgcgcg cgcccctgcc cccgtcgtgt tcatcgccga cggccggccc
      781 gagcacctgt cggactggca cctgctcaac gtgtccggcg gccggctgac gcccaatgcc
      841 ggcgtgctgc cctacgacct cgtcacgccg ctgttcagcg actacgcgca caagctgcgc
      901 acggtgtgga tgccccaggg cgtatcggcg aagtactcgc ccgaccaggc cttcgacttc
      961 ccggtcggca ccgtcctgtc caagaccttc tactacccgc gcgccggaac cgccgatgcc
     1021 gtcctcaaga cctacgacgc cgagccgggc gcgcccgccg cgggcctgga tctttcgcgc
     1081 gtgcacctgg tcgagacgcg cgtgctcgtg cgccgggccg agggctggac cgtgctgccc
     1141 tacgtgtgga acgccgccca gaccgaggcc gacctgcagc gcacgggcga cacgcagacc
     1201 ctcgagctgg tggatgcgca gggccacggc gagaagttcg cgtatgtcgt gcccaacgag
     1261 aaccagtgcg cctcgtgcca cgtcaccaac ctccggggcg ggacgttcac gccgctgggt
     1321 ccgaaggcgc gcaacctcaa ccgcacgctc gcctatgccg acggttccga gaaccagatc
     1381 gcgcgctggg tgcgcgccgg cttcctgacc ggcgtgcccg ccgtcgagct gccgcgcgcg
     1441 gcggactggc gcgacgtgca gcagccgatc gcggcgcgcg cccgcagcta cctcgacgtc
     1501 aactgcgcgc actgccacca gaacgcgggt accgcgagca acacctcgct gcgcctggac
     1561 gcgctcggcc cggccgactg gtcgctgggc atctgcaagc cgtcggtggc cgcgggcaag
     1621 gggacgggcg accgcatcga cgacatcgtg cccggcaagc ccgacgaatc catcctgccg
     1681 ttccgcctcg ccagcaccga gggcggcgtg atgatgcccg agctgggccg caacaccgtc
     1741 caccaggaag gcgtgcagct gatccgcgac tggatcgcct cgatgcctcc gggatgcaag
     1801 acagacaacc ccaccaagga ctgacatgca acgacaccct gcccgccgtc cgacctccct
     1861 tgccctggcc gccgtcgcgg ccgtgctcgc gggcgccagc gccggcgcgc gcgcgcaggc
     1921 ggccgcgccc gaggccgccg cgtccgcgcc ggccggcgac gtgcaggtga tcgaggtcac
     1981 ggcgcaggga cgcaagcagg aagtccagaa ggtgccgatc tcgctgcagc tggtcgggcc
     2041 ggacgagatc gccaagtccg gcgcggtcaa cctgtcgcag gtggccgact tcgtgccggg
     2101 cctgagcgtc gacgcgagcc agcccacgca gcccagctac gcgctgcgcg gcctgggcaa
     2161 cggcgacttc ggcatcggca ccgacgcgcc ggtgggcgtg tacgtcaacg gaatctatac
     2221 cggcaagacc ggcggcgcga tgctcaactt caacgacgtc aagcgcgtcg aggtgctcaa
     2281 gggcccgcag ggcacgctgt tcggccgcaa cagcgccggc ggggcgatct ccatcgtcac
     2341 caacgacccg gatggccagc gcacggcctc gggcctggtg cgcgtgggca actacggcac
     2401 ggtgcacgcc gagggcgtcc tgaaccagcc gctcggcgag gacttcagca tccgcctgtc
     2461 ggccgtcggc gagaacagcg acggctgggc caccaacacg gtcgacggca agaagagccc
     2521 gcaggcgcac acctggggca cgcgtctggg cgcgcgctgg tcgcccagcg attcgaccag
     2581 cgtcgtgctg acctgggagc acgaggacct gaacgagcgc ccgcgcccgg tctgggccgt
     2641 ggccacgtcg gacggcgccg gcggcgtcgc ctcgtggatc gacccggtcc acgcgccgct
     2701 gtccaacgac gtccagggtg gcgtcgagtc gcggctgttc aatggcatga acctgcgcgt
     2761 cgagcacgag ctcaccgaca aggtgctgct gacgtcgacc accggctggc gtcacttcga
     2821 ctcggtcaac ctcgaggaca acgacggcac ggcgaaccct gccacgtacc tggccaccgg
     2881 caacttcgag aagagcacga ccttcgagca ggagttccgg ctcaacggca agagcaagct
     2941 ggccgactgg ctggtcggcg cgacctttgc gtcggagcat tcggcgcaga ccagccgcgt
     3001 ggacaccaac accaccacgc tcgaccccgt gctgttcaac gtgctcaacg gcgagggctt
     3061 cgggctgccc gcgccgccgt tctcgctgct gaccggcatc ggacagcagg cgggcgtccc
     3121 gggcttcgac ctgctgggcc aggactggaa ggaaggcatc cagaacacgc tgtcctcgca
     3181 ttccgaggcg ctgatgggcg acgtcatctg gcacgtcggc gacaagacca acgtgacgac
     3241 gggcgtgcgc ttcacgcagg acaccaagaa gttcagctgg tacaacccgc tgcgctcggc
     3301 gccgggcctg gacacgcagc tggcgatcat gtcgccggcc ttcttccagc agctcgtcga
     3361 cgccggcgcg ctcgactcgg ccaccgcggc gcagctgacc cagctcgcgc agggcctgca
     3421 ggcgaccaac atcgagttca ccaatccggc ctgggcggcc gcgccggtct acacgagcaa
     3481 gacctggcac aacaccagcc cccgcctggt gatcgaccac cagctcacgc ccgacaccct
     3541 ggtgttcggc tcggtgacgc gcggctacca ggccggcggc ttcaactcgg tcagcaccga
     3601 cgccaacggc ggccgcttcg accccgagac gatcaccagc tacgagctcg gcgccaaggg
     3661 ccgcgtgccg ggcgccggca tcagctacag cgcctcgctc ttccactacc tgttcaagaa
     3721 cctgcagtcg atcacgctgg accattcgac cacggtgccg gtgtacgtca tcacggtcag
     3781 cgacgtcgag gccacgggcc tggacggcga gctgaactgg caggtggcgt cgccgctgcg
     3841 gctgtacgtc aacggcgagt ggatcgacca gaagtacaag tcgcaggaga acctcgcgcc
     3901 gggcgccaac gacctcaccg gccagcccta cggcacgccc agcctgtcgc tggccgccgg
     3961 cgtcgacgtt agcgtcgcgc tggcgggcgg caaggcggcc tggtcgctgc agggcagcca
     4021 catcggggcc acgcgctgca acgacgactc gcaggcccag ggcgcctgcg cgacgacgcc
     4081 ggcgttcaag gtgggccagg cgcgcgaccg cttcgacacc cgcctgggct gggccaacga
     4141 ccactggggc gcggcgctga tcgtcaccaa cctgttcgac aagcgctacg tcagcggcct
     4201 gagcaacctg tcggccgccg tgggcgtgcc ctacacggcc tcggtcagcg acccgcgcaa
     4261 gttccagatc gagttcagcg ccaagctgta ggcgtgcctt tctcgagcgg ccagcctgcc
     4321 ggcgacgctt gggattgtcc ttggggggcg gtgggagccg cccccgtttc ttttccgcgc
     4381 cgcggatgcg cgggccccgg ggcggggcgg cccggagaat ctgggcggcg tgcccagatt
     4441 aatgccgcga gtatcgaggg gataccgcga tcgcggtatt ggggcgtgaa aagtacccgc
     4501 ccgcaagggc ttgcggtttc cgtcgccata ttcctttgct gtaatgcggt ccagttcgca
     4561 gcggatgcag cccgccgcga agtgttgaac cctccacaat cggctgacct tcaggttcca
     4621 ccccggaacg tcccgaagac gctgattgac tcttggggtg ccgacgagtc gtcggcaccc
     4681 cgctttttta tccggcgggc gggttcgacg ctcgcgccat gtttcccccg ggccggcctc
     4741 tgcccgccga aaacatttgt ggcgccgtgc ccatcagatt cgaacaattc acgggccata
     4801 ccgagatcca ggggattggc agtccgtcgt gaagaatcca ggtcacgggt ttggcgccgc
     4861 gtgcgatgct cggttgcggc atcgcgctcg cccggcgtca tcctgcgact tcgcttcgct
     4921 gcgcgcagga tgacggcttg gcgttgcgcg tgtcgcgcta tcgacgctcg gcgtgccgtc
     4981 ctgtcgcgaa tcgtgatcga ctcaggtcga tttctcgacg cgcgctggct caccccatga
     5041 gcctttgccc ggccagaatg ccctggcagt gagtctcgcc acccgactcg tccgcgccgt
     5101 gtttcgaccg gatttcgctc ggcattagcg acaatgcggg aaatccccca cgaacaaagc
     5161 ccggtgaagt cagctaacac ttcactttca tagccacgcg caagtcctta atttagaacg
     5221 acattcgctt gcaaaacgtc atctaagtac ttgatttcat tgattttttc aggcgatgac
     5281 gcgttgaccc cgcccgggtg agtcgttaaa gtgcatcaaa gtgcactttt gtggtgggtc
     5341 gtggcatctc tgatttttga aggtccttca gcgttgacgc tggacggcaa ggggcgagtc
     5401 tcc
//
LOCUS       DATCRO010000912         8281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_44147, whole genome shotgun sequence.
ACCESSION   DATCRO010000912 DATCRO010000000
VERSION     DATCRO010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44147"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VIN75_24755"
     CDS             complement(<1..618)
                     /locus_tag="VIN75_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977448.1"
                     /translation="MAKTRVVVVDDSALVRSLLTEIINRQTDMECIGAASDPYAAREM
                     IRNLNPDVITLDVEMPRMDGIDFLSKLMRLRPMPVVMVSTLTERGAEVTLKALELGAV
                     DFVAKPKIGVADGLKQLAEEITDKIRTASKARVSKPHTPPVTSTVAGAALPAKPAVPV
                     SIGRLSTEKIIFIGASTGGTEATKEVLMNLPPDSPAVVITQHMPPG"
     gene            complement(640..1326)
                     /gene="cheD"
                     /locus_tag="VIN75_24760"
     CDS             complement(640..1326)
                     /gene="cheD"
                     /locus_tag="VIN75_24760"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEY8977449.1"
                     /translation="MASLQPTPTAPARPAPPTLRATTGPGSRLERLKSVTRKPGEASF
                     FFYDAHFQNEAVKVLPGEYYVDNEELLVMTTLGSCIAACLYDRQARIGGMNHFMLPEG
                     AGDSGRYGTYAMELLINEMMKRGASRMTMEAKVFGGGAVIAGMNSINVGERNTKFVMD
                     FLSTERIPVVSKDVLEIYPRKVCFLPASGKAMVKRLAPTNTEALVQQDRVAAQKAAPA
                     STGGGSIDLF"
     gene            complement(1389..2234)
                     /locus_tag="VIN75_24765"
     CDS             complement(1389..2234)
                     /locus_tag="VIN75_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8977450.1"
                     /translation="MSNDLAFAAADAASHEFTFSADDFRRVQKLIYQRAGISLHDGKR
                     AMVYSRLSRRLRETGDRSFADYLNGLERASGAAGEPEWQEFINCLTTNLTSFFREEHH
                     FHALADALKGLGGRPVRMWCNAASTGEEPYSIAMTLIENGCAGSRLLATDIDTKVLAT
                     AQRGVYPADSRGLSQQRLQKHMMRGTGANAGFMRVKPELQKMIEFRTFNLMATQWSLG
                     EPFDMVFCRNVMIYFDHDTQRRVLEHIHGVMKPGGLLFVGHSENFTDSRDLFVLQGKT
                     IYKRV"
     gene            2442..3173
                     /locus_tag="VIN75_24770"
     CDS             2442..3173
                     /locus_tag="VIN75_24770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8977451.1"
                     /translation="MNSKPPFLQTAPPDALRLEQALSLLAQSGQGAPAGAQGSTQWLQ
                     SLIDALCDLSSRDGLTGLANRRNFENAIARESDRVARSGEPALLLMLDIDHFKRINDQ
                     HGHVAGDLVIKAVSEALSATVRPMDLVARFGGEEFAIILPNCPPAFGTTVAERIRERV
                     ESRAIPLVSGQQLGCTISIGGAYAPQWVRSTPSVWMERADLQLYRAKSEGRNRTCLEP
                     TPVSVVSAEEKGLLFASSIFGEDLS"
     gene            3232..4014
                     /locus_tag="VIN75_24775"
     CDS             3232..4014
                     /locus_tag="VIN75_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEY8977452.1"
                     /translation="MNTAAAASGRRARITAITSGKGGVGKTFLSANLATALARQGEKV
                     LVLDADLGLANLDVVLNLFPKITLHDVFTGKSTLDQAILETSGGFSVLLAGSGMVEYS
                     RLTPEVREQLTDVIRQVVPRFDRILLDTGAGISDVVLYTVSLADEVLIVATPEPTSLT
                     DAYATIKVLATTQTRRDIRVLVNQVSKPGEGRVIRGQLQQVVDRFVSPGLEVPVKLEF
                     VGEVPIDPSVRDAVKKRQLLLECLPGSAAAQAVIAAATRLRA"
     gene            4082..4510
                     /locus_tag="VIN75_24780"
     CDS             4082..4510
                     /locus_tag="VIN75_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY8977453.1"
                     /translation="MTDSVTDAAEARQPTPFELLGGLDGVRALVDRFYDLMDLEPEFA
                     ELRALHPTTLEGSRDKLAWFLTGWLGGPDLYIERFGHPRLRMRHFPYAIGIRERDQWL
                     TCMGLAMAEIGEAGGFTACMQERLLQSFANTADHMRNRAG"
     gene            4852..5967
                     /locus_tag="VIN75_24785"
     CDS             4852..5967
                     /locus_tag="VIN75_24785"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8977454.1"
                     /translation="MQLLPLQEVASQIKPGLPLAWGIRDENGKLLLARGHRVTDVAML
                     NALLARGMFVDAAEMRAAMSSHFASKPREGFFGRWRLSSARLNTLLTTNPAELRDALV
                     EVVDVLIALADRYPDKVLFQILRHDQTKLNSYGVSHSLHSAAVCCLSAKRMGWEGAQR
                     RTLVAAAMSMNLSMIELQGRLAVQAVPPNPEQRAAIDDHPLRSAEMLRAAGVECPLWL
                     AAVEQHHELADGSGYPKRLTDVGELAQALHYADVFTAKLSARSSRAPLMPNVAARQMF
                     TDSSGHPLAAALIKEFGMYPPGCFVRLASGEIAIVIHRGETVATPVVACLTNVAGQAM
                     MRPQRRETTDKANAIVALVADADVMVRVPWETLYPDD"
     gene            5996..6706
                     /locus_tag="VIN75_24790"
     CDS             5996..6706
                     /locus_tag="VIN75_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977455.1"
                     /translation="MDAPDPPPVDWVEPHGTARAALMLLHGLDMAPARLAPILASLKL
                     PALVALPHGPVERPDGLRAWWPVDDVARAARIAAGPADLHASHPPGRAPARDAVHAAA
                     RALRERAPGLPLVVAGFSQGAMLALDCVFHAPPLEVDALALWSASRLAFAEWRPALHR
                     LRGVRVELLHGRADANFAVDAGRSLHDALAAEGADVRWATFDGGHEIPLQAWVALRRL
                     VRDVASTPRTPAGPGRGL"
     gene            6962..>8281
                     /locus_tag="VIN75_24795"
     CDS             6962..>8281
                     /locus_tag="VIN75_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977456.1"
                     /translation="MTGGDDSRPLAAARSALAPQPVLVEAAGHTCVAWWHAPAPGCAQ
                     PLAVVLASSWGEEDLAGYAAQRALAVALAAAGLGTLRFEWPDTGDSSARTGVTTIADA
                     LAAFDAAARRALALSGRERLAFVGRRLGALLAAHAALARQDVDALVALEPVASGAAFV
                     REQPRLAAARPAPAIVPGAVVDPAELPVALGGFTLPARQLEALAALRWPAAATTSVLE
                     ALLVLPAGPGSRAAADALARLGVRVHEAVLGDASAGEVVDWLRERAADDSVLQGVGAI
                     DDFGVADAGNPVVHARLAAARLEAATTAVLSLAMADAPVWMRLRERGVALRERVVRID
                     DARGPVLAGVVSERDPAASGELAPPARHAIVLLSPDGQRRIGPHRLWVPWARHRAACG
                     DVVLRLDAAGNGDSAPAGPPSPRGAGDVARAVAWLRRELGVIACTVVS"
BASE COUNT         1108 a         2940 c         2974 g         1259 t
ORIGIN      
        1 gcccggcggc atgtgctgcg tgatgaccac cgccggcgaa tcgggcggca ggttcatcag
       61 cacttccttg gtggcctcgg tgccgccggt ggacgcgccg atgaagatga tcttctcggt
      121 cgacagccgg ccgatggaca ccggcaccgc cggcttggca ggcagcgcgg cgccggccac
      181 ggtgctggtg accggcggcg tgtgcggctt ggatacgcgc gccttggacg ccgtgcggat
      241 cttgtcggtg atctcctcgg ccagctgctt gaggccgtcg gccacgccga tcttgggctt
      301 ggcgacgaag tcgaccgcgc cgagctcgag cgccttgagc gtgacctcgg cgccgcgctc
      361 ggtcagcgtc gagaccatca ccaccggcat cggccgcagg cgcatcagct tggacaggaa
      421 atcgatgccg tccatgcgcg gcatctccac gtccagcgtg atgacgtcgg ggttgaggtt
      481 gcggatcatc tcgcgcgccg cgtacgggtc gctggcggcg ccgatgcatt ccatgtcggt
      541 ctgccggttg atgatctcgg tgagcaggct gcgcacgagc gccgaatcgt ccaccaccac
      601 cacgcgggtc tttgccatgt ccgtgtccct tcctgcggat cagaacaggt cgatcgagcc
      661 gccgccggtc gacgccgggg ccgccttctg ggcggcgacg cgatcctgct gcaccagcgc
      721 ctcggtgttg gtcggcgcga ggcgcttgac catcgccttg ccgctggccg gcaggaaaca
      781 gaccttgcgc ggatagatct cgagcacgtc cttggacacc accgggatgc gttccgtgct
      841 gaggaagtcc atcacgaact tggtgttgcg ctcgccgacg ttgatcgagt tcatgccggc
      901 gatgacggcg ccgccgccga acaccttggc ctccatcgtc atgcgcgagg cgccgcgctt
      961 catcatctcg ttgatgagca gttccatcgc atacgtgccg tagcggccgg agtcgccggc
     1021 gccctcgggc agcatgaaat ggttcatgcc gccgatcctg gcctggcggt cgtacaggca
     1081 ggccgcgatg cacgagccga gcgtggtcat gaccagcagc tcctcgttgt cgacgtagta
     1141 ctcgccgggc agcaccttga ccgcctcgtt ctggaagtgc gcgtcgtaga agaagaacga
     1201 cgcttcgccg ggcttgcgcg tgaccgactt cagccgctcg aggcgcgagc cggggcctgt
     1261 ggtcgcgcgc agcgtgggcg gcgcggggcg ggccggggcg gtaggcgtgg gttggaggga
     1321 agccatggac tgcagcctga ggttttcttg ggaatttccg gtcgcggcgg gcgggagggg
     1381 cgcccgcgtc agacgcgctt gtagatggtc ttgccttgca ggacgaacag gtcccgcgag
     1441 tcggtgaagt tctcggagtg gccgacgaac agcaggccgc ccggcttcat cacgccatgg
     1501 atgtgctcga ggacgcggcg ctgcgtgtcg tggtcgaaat agatcatcac gttgcggcag
     1561 aagaccatgt cgaacggctc gcccagcgac cactgcgtgg ccatcaggtt gaacgtgcgg
     1621 aactcgatca tcttctgcag ctcgggcttg acgcgcatga agccggcgtt ggcgccggtg
     1681 ccgcgcatca tgtgcttctg caggcgctgc tgcgacagcc cgcgcgagtc ggccgggtag
     1741 acgccgcgct gcgcggtggc gagcaccttg gtgtcgatgt cggtggccag cagcctggag
     1801 cccgcgcagc cgttctcgat cagcgtcatc gcgatcgagt agggctcctc gccggtggag
     1861 gccgcgttgc accacatgcg caccgggcgg ccgcccaggc ccttgagggc gtcggccagc
     1921 gcgtggaaat ggtgttcctc gcggaagaag gaggtcaggt tggtggtcag gcagttgatg
     1981 aactcctgcc actcgggctc gccggcggcg ccgctggcgc gctccaggcc gttgaggtag
     2041 tcggcgaagc tgcggtcgcc ggtctcgcgc aggcggcgcg acaggcggct gtagaccatg
     2101 gcgcgcttgc cgtcgtgcag gctgatgccg gcgcgctggt agatgagctt ctggacgcgc
     2161 cggaagtcgt cggcgctgaa cgtgaattca tggctggcgg cgtcggcggc ggcgaatgcg
     2221 aggtcgttgc tcatcgtttt cttgaactgg cccggaaccg ggggatcggt atggggttat
     2281 cggtcgctcc cggccagtct caagactcct gcccatccac ggagccccga gttggggcgg
     2341 atttgcgcct cttttcgcgt ttccgccccg catgacccgg gcggtagact cggctgcaag
     2401 tgaaatggcg cctgctccag gcgtccgaga gtcgatccac agtgaactcg aagccccctt
     2461 tcctgcaaac cgccccgccg gacgcgctgc gcctggagca ggcgctgtcg ctgctggcgc
     2521 agtccgggca gggcgcgcct gcgggcgccc agggatcgac gcagtggctg cagtcgctga
     2581 tcgacgcgct gtgcgacctg tccagccgcg acggcctgac cggcctcgcg aaccggcgca
     2641 acttcgagaa cgcgatcgcc cgcgagtccg accgcgtggc gcgctccggc gagccggcgc
     2701 tgctgctgat gctggacatc gaccatttca agcgcatcaa cgaccagcac ggccacgtcg
     2761 ccggggacct cgtcatcaag gccgtgtccg aggcgctcag cgccaccgtg cgaccgatgg
     2821 acctggtcgc gcgcttcggc ggcgaggaat tcgccatcat cctgcccaac tgtccgcccg
     2881 cgttcggcac gaccgtggcc gaacgcatcc gcgaacgcgt cgagagccgc gccatcccgc
     2941 tggtcagcgg ccagcagctg ggctgcacga tcagcatcgg cggcgcctac gccccgcagt
     3001 gggtgcgttc gacgccgtcg gtgtggatgg aacgggccga cctccagctc taccgcgcaa
     3061 aatcagaagg ccgcaaccgc acctgcctcg agccgacgcc cgtgtccgtc gtctcggccg
     3121 aggagaaggg gctgctgttc gcgtcgtcca tctttgggga agacctctca tgagcccgcc
     3181 cggccgcccg aagggcgaat acagcgcagt gcacagcgcg gagcgttcac tatgaatacg
     3241 gcagccgccg cctccggacg acgcgctcgc atcaccgcca tcaccagcgg caagggtggc
     3301 gtgggcaaga ccttcctgtc ggccaacctc gccaccgcgc tggcgcggca gggcgagaag
     3361 gtgctggtgc tcgacgccga cctgggcctc gcaaacctcg acgtcgtgct gaacctgttt
     3421 cccaagatca cgctgcacga cgtgttcacc ggcaagagca cgctcgacca ggccatcctc
     3481 gagacgagcg gcggcttctc ggtgctgctg gccggctccg gcatggtcga gtactcgcgc
     3541 ctgacgcccg aggtgcgcga gcagctgacc gacgtcatca ggcaggtggt gccgcgcttc
     3601 gaccgcatcc tgctcgatac cggcgccggc atctccgacg tcgtcctgta caccgtgtcg
     3661 ctggccgacg aggtgctgat cgtggccacg cccgagccca cctcgctgac cgacgcctac
     3721 gccaccatca aggtgctggc caccacgcag acccggcgcg acatccgcgt gctggtcaac
     3781 caggtcagca agcccggcga ggggcgcgtc atccgcggcc agctgcagca ggtggtcgac
     3841 cgcttcgtca gcccggggct ggaagtgccg gtcaagctgg agttcgtcgg cgaggtgccg
     3901 atcgacccat cggtgcgcga tgccgtcaag aagcgccagc tgctgctcga atgcctgccc
     3961 ggctccgccg cggcccaggc cgtcatcgcc gccgccacgc gcctgcgggc gtgacgcgcg
     4021 agcccgcgcg ccgcgtgccc ccacgcgcac gcgcgggcga gccgcccgcg acaatgcggc
     4081 catgaccgat tccgtgaccg acgccgccga ggcgaggcag ccgacgccgt tcgagctgct
     4141 gggcggcctc gacggcgtgc gcgcgctcgt cgaccgtttc tacgacctga tggacctcga
     4201 acccgagttc gccgagctgc gcgcgctgca cccgaccacg ctcgagggct cgcgcgacaa
     4261 gctggcctgg ttcctcaccg gctggctggg cggccccgat ctctacatcg agcgcttcgg
     4321 ccacccgcgc ctgcgcatgc gccacttccc gtacgcgatc ggcatccgcg agcgcgacca
     4381 gtggctgacg tgcatgggcc tggcgatggc cgagatcggc gaggccggcg gcttcacggc
     4441 atgcatgcag gagcgcctgc tgcagtcgtt cgccaacacg gccgaccaca tgcgcaatcg
     4501 cgcgggctga tcccgatggg tcggggtcag gtcttgaatc gtgcagtgca cgtttcaaga
     4561 cctgaccccg caatttttta gacctgaccc cgcgcgacac ccgcgcgatc cggcgttgcg
     4621 ttcgatttgt tcagttgcat ccgagctgaa cgaatgtccg ccattccgcg aggtccgacg
     4681 cgtgtcgtcg agatctcctg acagcgcccg ccatcgtgat ttagtaggcg agatggcgct
     4741 ccgccgcacg cgcaagactg tggccgctcg cggatcatcc gatgacacga acatgcgggc
     4801 cccgcgaagc acgggccgcg cgtccgacaa cggatacgag ggtgtccagc catgcagttg
     4861 ttgccgctcc aggaagtcgc gagccagatc aagcccggcc tgcccctggc gtggggcatc
     4921 cgggacgaga acggcaagct gctgctcgcg cgcggccacc gcgtgaccga tgtcgcgatg
     4981 ctcaacgcgc tgctcgcgcg cggcatgttc gtcgacgccg ccgagatgcg cgccgcgatg
     5041 tccagccact tcgccagcaa gccccgcgag ggcttcttcg gtcgctggcg cctctcgagc
     5101 gcgcgcctga acacgctgct gacgaccaat ccggccgagc tgcgcgacgc gctcgtcgaa
     5161 gtggtggacg tgctcatcgc gctggccgac cgctatccgg acaaggtgct gttccagatc
     5221 ctgcgccacg accagaccaa gctcaattcg tatggcgtgt cgcactcgct gcacagcgcc
     5281 gcggtgtgct gcctgtcggc caagcgcatg ggctgggagg gcgcgcagcg ccgcacgctc
     5341 gtggccgcgg cgatgtcgat gaacctgtcg atgatcgagc tgcagggtcg cctggccgtg
     5401 caggccgtgc cgcccaaccc cgagcagcgc gccgcgatcg acgaccaccc cttgcgcagc
     5461 gccgagatgc tgcgcgccgc cggcgtcgag tgcccgttgt ggctggccgc cgtcgaacag
     5521 caccacgagc tcgccgacgg cagcggctac ccgaagcggc tgaccgacgt cggcgagctc
     5581 gcgcaggcgc tgcactacgc cgacgtgttc accgccaagc tcagcgcgcg ctcgtcgcgc
     5641 gcgccgctga tgcccaacgt cgccgcgcgc cagatgttca ccgacagctc gggccacccg
     5701 ctggctgcgg cgctgatcaa ggaattcggc atgtacccgc cgggctgctt cgtgcggctc
     5761 gcgtcgggcg agatcgcgat cgtgatccat cgcggcgaga ccgtcgccac gccggtggtc
     5821 gcctgcctga ccaacgtcgc cggccaggcg atgatgcggc cgcagcgccg cgagaccacc
     5881 gacaaggcca acgccatcgt ggcgctggtc gcggacgcgg acgtgatggt gcgcgtgccg
     5941 tgggagacgc tgtaccccga cgactgaccg cgcggcgcgc gccgacaatg gcgcgatgga
     6001 cgcgcctgat cctccgcccg tcgactgggt cgaaccgcac ggaacggcgc gcgccgcgct
     6061 gatgctcctg cacggcctgg acatggcgcc cgcgcgcctg gcccccatcc ttgcatcgct
     6121 gaagctgcct gcgctggtgg cgctgccgca cggccccgtc gagcggcccg acggcctgcg
     6181 tgcctggtgg cccgtcgacg acgtggcacg cgccgcccgc atcgccgcag gccccgccga
     6241 cctgcacgcc agccacccgc cgggccgcgc gcccgcgcgt gacgcggtgc acgcggccgc
     6301 gcgcgccctg cgcgagcgcg cgcccgggct gccgctggtg gtggccgggt tctcgcaggg
     6361 cgcgatgctg gcgctcgact gcgtgttcca tgcgccgccg ctcgaggtcg acgcgctggc
     6421 actgtggtcg gcctcgcgcc tcgcattcgc ggagtggcgg ccggccctgc accggctgcg
     6481 cggcgtgcgg gtcgaactgc tgcatggacg ggcggacgcg aacttcgccg tcgatgccgg
     6541 ccgctcgctg cacgacgcgc tggcggccga gggtgccgac gtgcgctggg cgacgttcga
     6601 cggcggccat gagatcccgc tgcaggcctg ggtggcgctg cgccgcctcg tgcgtgacgt
     6661 ggcgtcgacg ccgcggacgc ccgcaggacc gggccgcggg ctgtgactta actcgcaggg
     6721 ggcgggaatt tcgcatcgtg gacggggtga ggcgacggct tggcggtcga gatgcgtgcg
     6781 cacgacctct agaatcgttt gacacgtttt gtaactacac cgccgtgcgt cgaccgtcca
     6841 ccccgaacct gccgctgctg gcgcctgtga aggcgctgcg tggcctcgcc gccggggccg
     6901 atgggatccg ggcgccgcgg gacaaggacg cggctcgcgc gccccgccgg cccacccgat
     6961 cgtgaccggc ggcgacgact ccaggcccct cgccgcggcg cgctcggccc tcgcgccgca
     7021 gccggtgctg gtcgaggccg cgggccacac ctgcgtcgcc tggtggcacg cgcccgcccc
     7081 gggctgcgcg cagccgttgg cggtggtgct cgcgagctcg tggggcgagg aagacctggc
     7141 aggctatgcc gcccagcgcg cgctcgccgt cgcgctcgcc gcggcgggcc tgggcacgct
     7201 gcgcttcgaa tggcccgaca ccggcgactc gtcggcgcgc acgggggtga cgaccatcgc
     7261 ggacgcgctc gccgccttcg acgccgcggc gcgtcgcgcg ctcgcgctgt ccggccgcga
     7321 acggctcgcg ttcgtcgggc gccggctcgg cgcgctgctg gcggcacatg cggcgctcgc
     7381 gcgccaggac gtcgatgcgc tggtggcgct ggagcccgtg gccagcggcg ccgcgttcgt
     7441 gcgcgagcag ccacggctgg ccgcggcgcg cccggcgccc gcgatcgttc caggcgccgt
     7501 ggtcgatccg gccgaactgc ccgtggccct gggcggcttc acgctgcccg cgcgccagct
     7561 ggaggcgctg gccgcgctgc gctggccggc ggcggccacc acctcggtgc tggaggcgct
     7621 gctcgtgctg ccggcgggac cgggcagccg cgcagccgcc gacgcgctgg cgcgcctggg
     7681 cgttcgcgtg cacgaggccg tgctgggcga cgcgagcgcc ggcgaggtcg tcgactggct
     7741 gcgcgaacgc gccgccgacg actcggtgct gcagggcgtg ggcgcgatcg acgacttcgg
     7801 cgtcgccgac gccggcaacc cggtcgtgca tgcgcgcctg gccgcggcgc ggctggaggc
     7861 cgcgacgacg gcggtgctgt cgctcgcgat ggccgacgcg ccggtgtgga tgcgtctgcg
     7921 cgagcgcggc gtcgcgctgc gcgagcgcgt ggttcgcatc gacgacgcgc gcgggccggt
     7981 gctggcgggt gtcgtgtcgg agcgcgatcc ggcggccagc ggcgagcttg cgccgcccgc
     8041 gcgccatgcc atcgtgctgc tgtcgcccga cggccagcgc cgcatcgggc cgcaccggct
     8101 gtgggtcccc tgggcgcgcc accgcgccgc gtgcggcgac gtcgtgctgc ggctcgatgc
     8161 ggccggcaat ggcgacagcg cgccggccgg gccgccgtcc ccacggggcg ccggcgacgt
     8221 cgcgcgcgcc gtcgcgtggc tgcggcgcga actgggcgtc atcgcgtgca ccgtcgtcag
     8281 c
//
LOCUS       DATCRO010000913         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_427054, whole genome shotgun sequence.
ACCESSION   DATCRO010000913 DATCRO010000000
VERSION     DATCRO010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427054"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..730)
                     /locus_tag="VIN75_24800"
     CDS             complement(104..730)
                     /locus_tag="VIN75_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY8977457.1"
                     /translation="MRSLQDLGAMREALARAQREAAEAARKAAEEAARLDRERRQFEL
                     AVGAVKPVKAAPRADVRPPAPPALPRQRELDEARALAEAMSDEVDVESLLLTDDGLGF
                     RRPGVSLDVLAQLRKGKWAIQDQLDLHGMRRDEAREAVGAFIRAAGQRGQRCLRVIHG
                     KGHGSPGREPVLKSKVHKWLAQRQEVIAFAQATGPQGGAGAVIVLLES"
     gene            878..>2064
                     /locus_tag="VIN75_24805"
     CDS             878..>2064
                     /locus_tag="VIN75_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY8977458.1"
                     /translation="MRTQPRISGLSGNEIYCMHLKGFEPVGIVVGNSVRSMGLLGGLT
                     AGVRGVIGGEIPEITQIIHEGRQSAYERMVAEVKQGSAHGAVGVTSELRNLAGNTEYL
                     FVGSSVRQSGVDEFFTSAGDAQELYCHMDAGYKPLRYVFGNIAYSMGAVGGIVGGLKT
                     MVRGEIKEFSDIFNATRHLALQRVVDEARRAGANAVVGIRTKVERFNGAHEMFMAGTA
                     VAHPALAEHAGHPVTSDLTGEELWSLSALGFAPVKLVMSTSVYSLGAIGGLKAMFKGF
                     VKGEISDLTSMVYDAREHVFERLEREAGQVGADEIVGVKTYIIELGSGLIEFVAVGTA
                     VRKVVGTGTATAQLPPQAVIRDKDTWVSGDGGFDLQGTRARVTNAAPWRGWILRPRSA
                     PRR"
BASE COUNT          293 a          733 c          740 g          298 t
ORIGIN      
        1 cccttgggca ggtcccagcg cgaggtgccg gtggcgtggc acgcgaagac gtggtcgcgt
       61 tcgtcgacca gcaccacgcc gcacgagacc accggcgccg tcatcacgac tccagcagca
      121 cgatgaccgc gcccgcgccg ccctgcggcc ccgtggcctg agcgaacgcg atgacctcct
      181 ggcgctgcgc cagccacttg tgcaccttgg acttgagcac gggctcgcgc ccgggcgagc
      241 cgtggccctt gccgtggatg acgcgcaggc agcgctgccc gcgctggccc gcggcgcgga
      301 tgaacgcgcc gacggcttcg cgcgcctcgt cgcgccgcat gccgtgcagg tccagctggt
      361 cctggatcgc ccacttgccc ttgcgcagct gcgccagcac gtcgagcgac acgccgggcc
      421 ggcggaagcc caggccgtcg tcggtcagca gcagcgactc gacgtcgact tcgtcggaca
      481 tcgcctcggc cagcgcgcgc gcctcgtcga gctcgcgctg gcgcggcagc gcgggtggcg
      541 cgggcggtcg cacgtcggcg cgcggcgccg ccttgacggg cttgaccgcg cccaccgcga
      601 gttcgaactg gcggcgctcg cgatcgagcc gcgccgcctc ttccgcggcc ttgcgcgcgg
      661 cctcggccgc ctcgcgctgc gcgcgcgcca gcgcttcgcg catggcgccc aggtcctgca
      721 agctgcgcat tttcaagtcg atgtccctcc ggcccggccc gcgtcggccg gctcaattcg
      781 tcggccattt tcgtcgactg ggcggtgccc gggatcccgg atggattccc gtggggcaag
      841 atggttcccg tccagacgac gaggagagga tttccccatg cgcacccaac cccggatctc
      901 cggcctgtcc ggcaacgaga tctactgcat gcacctcaag ggcttcgagc ccgtgggcat
      961 cgtcgtcggc aacagcgtgc gctcgatggg cttgctcggc ggcctgaccg cgggcgtgcg
     1021 cggcgtcatc ggcggcgaga tccccgagat cacgcagatc atccacgagg gccgccagtc
     1081 ggcttacgag cgcatggtgg ccgaggtcaa gcagggcagc gcgcacggcg ccgtgggcgt
     1141 cacgtccgag ctgcgcaacc tggcgggcaa cacggaatac ctgttcgtcg gctccagcgt
     1201 gcgccagtcg ggcgtcgacg agttcttcac cagcgccggc gacgcgcagg agctctactg
     1261 ccacatggac gccggctaca agccgctgcg ctacgtgttc ggcaacatcg cgtactcgat
     1321 gggcgcggtc ggcggcatcg tcggcggcct caagaccatg gtgcgcggcg agatcaagga
     1381 gttcagcgac atcttcaacg cgacgcgcca cctggcgctg caacgcgtcg tggacgaggc
     1441 caggcgggcc ggcgccaacg cggtggtggg catccgcacg aaggtcgagc gcttcaacgg
     1501 cgcgcacgag atgttcatgg ccggcacggc cgtcgcgcac ccggcgctgg ccgagcacgc
     1561 cggccacccc gtcacgagcg acctcaccgg cgaggagctg tggagcctca gcgcgctggg
     1621 cttcgcgccg gtcaagctgg tgatgtcgac gtcggtctac tcgctcggtg cgatcggcgg
     1681 cctgaaggcg atgttcaagg gcttcgtcaa gggcgagatc agcgacctca cgtccatggt
     1741 ctacgacgcc cgcgagcacg tcttcgaacg cctcgagcgc gaggcaggcc aggtgggcgc
     1801 cgacgagatc gtgggtgtca agacgtacat catcgagctc ggctcgggcc tgatcgagtt
     1861 cgtcgcggtg ggcacggcgg tgcgcaaggt ggtcggcacg ggcaccgcga cggcgcaact
     1921 gccgccgcag gccgtgatcc gcgacaagga tacgtgggtc agcggcgatg gcgggttcga
     1981 cctgcagggc acgcgcgccc gggtgacgaa cgcggcgcct tggcgggggt ggatcctgcg
     2041 accgcgctcc gctccgcgca ggat
//
LOCUS       DATCRO010000914         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_213044, whole genome shotgun sequence.
ACCESSION   DATCRO010000914 DATCRO010000000
VERSION     DATCRO010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213044"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VIN75_24810"
     CDS             <1..487
                     /locus_tag="VIN75_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977459.1"
                     /translation="FEPQTRTLISGDALWEERLAIIFPELIGEDGFGPTRATLSLIER
                     LAPRTVLPGHGRVFEDVQRALAASRERVAAFERHPERHAQHAARALVMFHLLEVREVG
                     FVDLVAWMLRTPIYLAVARRSGLDDAAATAWAATQVRRLVDDGVLLEHEGRVAVQAHA
                     H"
     gene            477..1820
                     /locus_tag="VIN75_24815"
     CDS             477..1820
                     /locus_tag="VIN75_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977460.1"
                     /translation="MLTRTAASAVADPDDTTIPGEDFIDMSAPAPRLTTPAELRQQSD
                     APDRLVFFFSGFDPKSATFYHRLFRSGIAQRNAAHDETLALGKRHRIGRWASVWTSLW
                     RGAPTGLGRRPETMRTRIHFMRWDDIVRKHWKRTPLTLACDYWNVYARGLASGALVSV
                     WRGAPAAFRLALFPLLVGMASVLAGTLVAGGLLALATPLPGLAAAGAGAAAGLLLWRA
                     AARWVDGEWMLRLVAFMREHAIGEVPALDVRLDEMADRVVEAVEARMRHPGAAPLREV
                     MVLGYSSGTILAASVLARAMPRLAHIVAGHRADKGNTTLSLVTLGQCIPIATAWPQAR
                     RIRSELEILAESPSLTWHDYSASSDRAAFWNTPPWPEPTKMKGRQASPPFKAAPGGLK
                     FAALSRERRETHLQYLRPPVGKGDPSSFDFLTIACGPQTLAERHAKGETAGDLRG"
BASE COUNT          303 a          739 c          770 g          330 t
ORIGIN      
        1 gttcgagccg cagacacgca cgctgatctc gggcgacgca ctgtgggagg agcggctcgc
       61 gatcatcttt ccggaactga tcggcgaaga cggcttcggt ccgacgcgcg cgacgctgtc
      121 gctcatcgag cggctggccc cgcgcacggt gctgccggga cacggccgtg tcttcgagga
      181 tgtccagcgc gcgctggcgg ccagccgcga gcgcgtggcc gccttcgagc gccatcccga
      241 gcgccatgcg cagcacgcgg cccgtgcgct cgtgatgttc catttgctgg aggtgcgcga
      301 ggtcggcttc gtcgacctgg tcgcatggat gttgcgcacg ccgatctatc ttgccgtggc
      361 gcgacgttcg gggctggacg acgccgccgc gacggcgtgg gccgcgacgc aggtgcgccg
      421 gctcgtcgac gacggcgtgc ttctcgaaca tgaaggccgc gtggcggtgc aggcgcatgc
      481 tcactaggac cgccgccagc gccgtcgccg atcccgacga cacgacgatt ccgggcgagg
      541 atttcatcga catgtcggcg cccgcgccgc gattgacgac gccagcggag ttgcgccagc
      601 aatcggacgc gcccgaccgg ctggtcttct tcttcagcgg cttcgacccg aagagcgcca
      661 cgttctacca ccggctgttc cgcagcggca tcgcgcagcg caatgcggca cacgacgaga
      721 cgctggcgct cggcaaacgg catcgcatcg ggcgctgggc ttcggtctgg acatcgctgt
      781 ggcgcggcgc gcccacgggc ctggggcggc gacccgagac gatgcgcacg cgcatccact
      841 tcatgcgctg ggacgacatc gtccgcaagc actggaagcg cacgccgctg acgttggcgt
      901 gcgactactg gaacgtctat gcgcgcggac ttgcgtccgg cgcgcttgtc agtgtctggc
      961 gcggggcgcc ggccgcgttc cggctggcac tgttcccgtt gctggtgggc atggcctcgg
     1021 tactggccgg cacgctggtc gcgggcgggc tgctcgcgtt ggcgacgccg ttgccaggcc
     1081 tggcggcggc cggtgccggc gcggcggcgg gcctgttgct gtggcgcgcg gcggcacgct
     1141 gggtcgacgg cgagtggatg ctgcgcctgg tggccttcat gcgcgagcac gccatcggcg
     1201 aggtgccggc gctcgacgtc cgcctggacg agatggccga ccgcgtggtc gaggcggtgg
     1261 aagcgcgcat gcgccatccc ggcgcggcgc cgctgcgcga ggtcatggtg ctgggctaca
     1321 gctcgggcac catcctggcc gccagcgtgc tggcccgcgc gatgcccagg ctggcccaca
     1381 tcgtcgccgg ccaccgagcg gacaagggca acacgacgct gtcgctcgtg acactgggcc
     1441 agtgcatccc gatcgccacg gcctggccgc aggcgcgtcg catccgcagc gagctcgaga
     1501 tcctggccga gagcccgtcg ctgacgtggc acgactactc ggcatcctcg gatcgcgcgg
     1561 cgttctggaa cacgcccccg tggccggagc cgacgaagat gaagggccgg caggcgtcgc
     1621 caccgttcaa ggcggctccc ggcggcctga aattcgcggc gctcagccgg gagcgtcgcg
     1681 agacgcacct gcagtacctg cgcccgcccg tcggcaaggg cgatccatcc tcgttcgatt
     1741 tcctcacgat cgcctgcggg ccgcagacct tggccgagcg ccacgcgaag ggggagacgg
     1801 cgggcgatct gcggggctga cgcgtgagcg cgatgcggac ggtccaagtc gattgatcgg
     1861 cctgggaagt cgtcactgcg ccatgactcg cgttccgcga cgcgtcacac gccccctggg
     1921 atcgcgtagg gcgggctagc cgcaggcgta acccgccgtt cggtggcgtc gcgacatgcc
     1981 ggcatggcgg gttacgcctg cgctaacccg ccctacaccg tgacgcgacg gcgtgccccc
     2041 aaaagcaaaa cgcccgagct agaaaactag ctcgggcggt tgctttgata ttagcctgac
     2101 aatgtcctac tttcacacgg gaacccgcac tatcatcggc gc
//
LOCUS       DATCRO010000915         6073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_988953, whole genome shotgun sequence.
ACCESSION   DATCRO010000915 DATCRO010000000
VERSION     DATCRO010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988953"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VIN75_24820"
     CDS             complement(<1..277)
                     /locus_tag="VIN75_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977461.1"
                     /translation="MANSAPASTLEVRRLDTVSQSQLLALADVLVDCVEGGASVSFML
                     PITRERALAFWRRVADGVARRERVLLVAEDAEGIVGTVQVILDQPENQ"
     gene            complement(246..878)
                     /locus_tag="VIN75_24825"
     CDS             complement(246..878)
                     /locus_tag="VIN75_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY8977462.1"
                     /translation="MKETPPASLNDRIAQRVRDLRAASGLSLDALATHCGISRSMISL
                     IERGESSPTAVLLEKLASGLGVPLAALFDAPEPTADPVARAADNPSWRDPHSGYVRRN
                     VSPSGVGSQIQIVEVRFPSKARVSYETGPREPVMHQQVWVIEGAIDITVGDEKHRLGE
                     GDCVAMVLDRPITFHNPHKTAARYAVVLASMPYPLPFRRSHGEQRPSLYP"
     gene            1210..2394
                     /locus_tag="VIN75_24830"
     CDS             1210..2394
                     /locus_tag="VIN75_24830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977463.1"
                     /translation="MFRPTHPPSSTTAHPLLARVQRWQSTVFALVAGGTTYTSGHGAA
                     GMIVAPVLVVAGVLLDRLRRARDTRTAAANAAFLDSTDRVGHDVLPVWAAHIESSRQQ
                     METAVAALTRRFGGIVDRLDQTSRAASQDGDDSLSAAFGQSRQDLQSILDSLREAMAS
                     NEAMNAEVQSLGRFVAELQQMAAEVADIAQQTNLLAINAAIEAAHAGAEGRGFAVLAQ
                     EVRKLSALSGATGQRMADKVAVIGEAIRVARDAAQASARREAASAQASEEAINGVLER
                     FRGVTESLEASAAVLKQENAGIQVEIVESLVQLQFQDRVSQRMAHVHHNIGRLPELLA
                     RSRRRFDESGTLAPVDAQELLDELEGSYAMADERATHAVQGAVAAPAAPAGAVLEEVT
                     FF"
     gene            2439..2807
                     /locus_tag="VIN75_24835"
     CDS             2439..2807
                     /locus_tag="VIN75_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977464.1"
                     /translation="MAKTIMIVDDSASLRQVVGISLRSAGYDVLEGADGADALKKLTG
                     QKVHLIISDVNMPNMDGISLVKEIKKLPAYKFTPIVMLTTESADGKKAEGQAAGAKAW
                     MLKPFNPPQLLAVVQKLVLP"
     gene            2884..3183
                     /locus_tag="VIN75_24840"
     CDS             2884..3183
                     /locus_tag="VIN75_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8977465.1"
                     /translation="MTSSSFRIDGELTIYRAAELAVAMKTALAGVAEGGALELDLSEV
                     AEMDCAGVQLLLAARRSATESGRSLRLAGRSAAVDEVLAILRLTGHFGDAVAGTH"
     gene            3193..5334
                     /locus_tag="VIN75_24845"
     CDS             3193..5334
                     /locus_tag="VIN75_24845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY8977466.1"
                     /translation="MANDINLDQVLQTFIVEARELLDDMEAALLRVAGEADPRESINA
                     IFRTAHTIKGSSGLFGLDAIVAFTHVAESVLDRVRDGAVMLDEALVALLLACGDYIGR
                     MVDAVERAEPQLDPRAEPEGAALLARLQVHLEAREAPAAVVAAAPAAAGGAPARWHLS
                     LRFGPDVLRNGMDPLSFIRYLATLGQIEHLVTIPDAVPALDALDPEGCCLGFEIAFLT
                     TADKAAIENVFEFVIDDCALRIVPPGSQVARYIELVQSLPEGPSRLGEILVRCGSVTA
                     HELEAALRLQARSAPSRKIGAILAEQGTVAPQVVEAALVRQKQAKDTRGGESQSVRVD
                     ADKLDRLINLVGELIIATAGANLVAKRTRNLELMERNSTLADLVEEVRDSALQLRMVK
                     IGATFNRFRRVVHDVARELGKDIELAVSGEDTELDKTVVEKIGDPLMHLVRNAMDHGI
                     DSPERRAERGKPATGTVSLNAFHDSGSIVIQVGDDGGGLDRERILAKGIERGLVEPGR
                     AMSDAEVYALIFEPGFSTAEQITNLSGRGVGMDVVRRNIEALRGNVAIASEPGRGTTV
                     TVRLPLTLAIINGFQVGVGKSVFVVPMDMVDECIEFSTETGQDYTDLRGQVLPFIRLR
                     ELFDVPGRPAVRQNIVVVKHAGQKFGLVVDALMGEAQAVIKPLSRMFVQVRGISGSSI
                     LGSGDVALILDVPVLMQQAQLSRGAAAHPAS"
     gene            5376..>6073
                     /locus_tag="VIN75_24850"
     CDS             5376..>6073
                     /locus_tag="VIN75_24850"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEY8977467.1"
                     /translation="MLKNLRVGTKLFGSFLVVLGLLAGLGVFALAKLANVNASALEIA
                     NQALPSVRAIVFANAHMNRVRTLQYKAVVVEPPEVPAVEKEAAAKLADLERVNADFAR
                     LIENPEERALFESYKSDVDAFVGENARIMDLARQNKDAEARAVLEGEGQKHYQAMRAA
                     CNKLVDLVTADGNRIAAAAQSTYDSARTWIIAAIVGCSALALLMATLITRGITLPVRA
                     AVDAANRLAEGDLT"
BASE COUNT          961 a         2106 c         2143 g          863 t
ORIGIN      
        1 gctggttctc gggctggtcg aggatgacct gcaccgtgcc gacgatgcct tcggcgtcct
       61 cggccaccag cagcacgcgc tcgcgccgcg cgacgccgtc tgccaccctg cgccagaacg
      121 ccagggcgcg ctcgcgggtg atcggcagca tgaagctgac cgaggcgccg ccttccacgc
      181 agtcgacgag cacgtcggcc agcgcgagca gctgcgattg gctgacggtg tcgagccggc
      241 ggacttcaag ggtagaggct ggggcgctgt tcgccatggg atctccggaa gggcaggggg
      301 tagggcatcg aggcgagcac cacggcatag cgcgcggcgg tcttgtgcgg gttgtggaag
      361 gtgatggggc ggtcgagcac catcgcgacg cagtcgcctt cgcccaggcg gtgcttctcg
      421 tcgcccacgg tgatgtcgat cgcgccctcg atcacccaga cctgctggtg catcaccggc
      481 tcgcgcgggc cggtctcgta cgacacgcgc gccttggacg ggaagcgcac ctcgacgatc
      541 tggatctgcg agccgacgcc gctgggcgac acgttgcgcc gcacgtagcc cgagtgcgga
      601 tcgcgccagg acgggttgtc ggccgcgcgc gccaccgggt cggccgtcgg ctcgggcgcg
      661 tcgaacagcg cggccagcgg cacgcccagc ccgctcgcga gcttttccag cagcaccgcc
      721 gtggggctgc tctcgccgcg ctcgatcagc gagatcatcg agcggctgat gccgcagtgc
      781 gtggccagcg cgtcgagcga caggcccgag gcggcgcgca ggtcgcgcac gcgctgcgcg
      841 atgcggtcgt tcaggctggc ggggggtgtc tctttcatgg tggagctagt gtccattatg
      901 ttggatgagg cgtcaaccgg accggacgac cgcggccgcg ggcacgttcc ggacgatacg
      961 tggcggcgta tctggaccgg cgctcggccg accgaagcgt cccgcagctg tggggaattg
     1021 tcgattcttc gtgtcagccg atctccatac ggcacgacgt atacggacga ccggcgatcg
     1081 acggcgaagg aatgcgcctc gaacggcccg gtattcgaat gctcgcgcga catgccgccg
     1141 tgaacaatgg gccggtcaac aaccaggaat cgggccttca gcgatcgcgg ctccggtcga
     1201 aaaccagcca tgttccgacc cacgcaccca ccgtccagca cgacggctca tccgcttctc
     1261 gcccgcgtcc agcgctggca gtcgacggtc ttcgcgctcg tcgcgggagg gaccacctac
     1321 acctccggcc acggcgccgc ggggatgatc gtggcaccgg tgctcgtggt cgccggcgtg
     1381 ctgctcgacc gcctgcgccg cgcgcgcgac acgcggaccg ccgcggccaa cgccgccttc
     1441 ctcgacagca ccgaccgcgt gggccacgac gtgctgcccg tctgggccgc gcacatcgag
     1501 agctcgcgcc agcagatgga gaccgccgtg gccgcgctga cgcggcgctt cggcggcatc
     1561 gtcgatcggc tcgaccagac ttcgcgcgcg gccagccagg acggcgacga cagcctctcg
     1621 gccgcattcg gccagagccg ccaggacctg cagtccatcc tcgattcgct cagggaggcg
     1681 atggccagca acgaggcgat gaacgccgag gtgcagagcc tgggccgctt cgtcgccgag
     1741 ttgcagcaga tggcggccga ggtcgccgac atcgcgcagc agaccaacct gctggccatc
     1801 aacgcggcca tcgaggccgc tcacgcaggc gccgagggcc ggggttttgc cgtgctcgcc
     1861 caggaggtgc gcaagctgtc ggcgctgtcg ggtgcgacgg gccagcgcat ggccgacaag
     1921 gtggcggtga tcggcgaggc gatccgcgtg gcgcgcgacg ccgcacaggc gtccgcccgg
     1981 cgcgaggccg cctcggcgca ggcgtccgag gaggcgatca acggcgtgct cgagcgcttc
     2041 cgcggcgtga ccgagtcgct cgaggcgtcg gccgccgtgc tcaagcagga gaacgcgggc
     2101 atccaggtgg agatcgtcga gtcgctggtg cagctgcagt tccaggaccg cgtgagccag
     2161 cgcatggccc acgtccacca caacatcggc cgcctgcccg aactgctggc acgcagccgc
     2221 cgccgcttcg acgaatcggg cacgctcgcg ccggtcgacg cccaggagct gctcgacgag
     2281 ctcgagggca gctacgcgat ggccgacgag cgcgccacgc acgcggtgca gggcgccgtg
     2341 gccgcgccgg ccgcgccggc cggcgcggtc ctcgaggaag tcaccttctt ctgatttctg
     2401 aaccttcttt cacgccaatc caggaacagg gaacccgaat ggccaagacc atcatgatcg
     2461 tcgacgactc cgcctcgctg cgccaggtcg tgggcatctc gctgcgcagc gccggctacg
     2521 acgtgctcga gggcgccgac ggcgccgacg cgctcaagaa gctcaccggc cagaaggtgc
     2581 acctgatcat cagcgacgtg aacatgccca acatggacgg catctcgctg gtcaaggaga
     2641 tcaagaagct gccggcgtac aagttcacgc ccatcgtgat gctgaccacc gagtcggccg
     2701 acggcaagaa ggcggaaggg caggcagccg gcgccaaggc gtggatgctc aagccgttca
     2761 acccgccgca gctgctggcc gtggtgcaga agctggtcct gccctgaacc gggccctctg
     2821 aaggcccatg gggcgacgcg cgagcgcgca ctagccaatg actgcaccgg aatcgcgaca
     2881 ggcatgactt catcctcctt tcgaatcgac ggcgaactga cgatctaccg cgcagcggag
     2941 ctggccgtgg ccatgaagac ggcgttggcc ggcgtggccg aaggcggtgc gctggagctg
     3001 gacctgtccg aggtcgccga aatggactgc gccggcgtgc agctgctgct ggcggcgcgg
     3061 cgcagcgcga ccgagtcggg ccgctcgctg cgcctggccg gccgcagcgc ggcggtggac
     3121 gaggtcctgg cgatcctgcg gctgaccggg cacttcggcg acgccgtcgc cgggacgcat
     3181 tgagggcacg ccatggccaa cgacatcaac ctggaccagg tcctgcagac cttcatcgtc
     3241 gaggcccgcg agctcctcga tgacatggag gccgcgctgc tgcgcgtcgc cggcgaggcc
     3301 gacccgcgcg agagcatcaa cgcgatcttc cgcaccgccc acacgatcaa gggatcgtcg
     3361 gggctgttcg gcctggatgc gatcgtcgcc ttcacgcacg tcgccgaaag cgtgctcgat
     3421 cgcgtgcgcg acggcgccgt catgctggac gaggcgctgg tcgcgctgct gctggcctgc
     3481 ggcgactaca tcggccgcat ggtcgacgcc gtcgaacgcg ccgagccgca gctcgacccg
     3541 cgtgccgagc cggaaggcgc cgcgctgctg gcgcgcctgc aggtccatct cgaggcgcgc
     3601 gaggcgccgg cggccgtggt cgccgcggcg cccgcagcgg ccggcggcgc cccggcgcgc
     3661 tggcacctgt cgctgcgctt cggccccgac gtccttcgca acggcatgga tccgctgtcg
     3721 ttcatccgct acctggccac gctcggacag atcgagcacc tcgtgacgat ccccgacgcc
     3781 gtgccggcgc tggacgcgct ggacccggag ggctgctgcc tgggcttcga gatcgccttc
     3841 ctcacgaccg cggacaaggc cgcgatcgag aacgtgttcg agttcgtcat cgacgactgc
     3901 gcgctgcgca tcgtgccgcc cggcagccag gtggcgcgct acatcgagct ggtccagagc
     3961 ctgccggaag gccccagccg cctcggcgag atcctggtgc gttgcggcag cgtcaccgcg
     4021 cacgagctcg aggcggcgtt gcgcctgcaa gcccggagcg cgccgtcgcg caagatcggt
     4081 gccatcctcg cagagcaggg caccgtcgcg ccgcaggtgg tggaggcggc gctggtcagg
     4141 cagaagcagg ccaaggacac gcgcggcggc gagagccagt ccgttcgcgt cgacgccgac
     4201 aagctcgatc gcctgatcaa tctcgtgggc gaactgatca tcgccacggc gggcgccaac
     4261 ctcgtggcca agcgcacgcg caacctcgag ctgatggagc gcaactcgac gctggccgat
     4321 ctcgtggagg aggtgcgcga cagcgcgctg cagctgcgca tggtcaagat cggcgccacg
     4381 ttcaaccgct tcaggcgcgt ggtgcacgac gtggcgcgcg agctgggcaa ggacatcgag
     4441 ctcgcggtca gcggcgagga cacggagctg gacaagaccg tcgtcgagaa gatcggcgac
     4501 ccgctgatgc acctcgtgcg caacgcgatg gaccacggca tcgactcgcc cgagcgtcgc
     4561 gccgagcgcg gcaagccggc cacgggaacc gtgtcgctga atgccttcca tgactcgggc
     4621 agcatcgtca tccaggtggg cgacgatggc ggcgggctcg atcgcgagcg catcctggcc
     4681 aagggcatcg agcgcgggct ggtggagccc ggtcgcgcga tgtccgacgc cgaggtctac
     4741 gcgctgatct tcgagccggg cttctcgacg gccgagcaga tcaccaacct gtcggggcgc
     4801 ggcgtcggca tggacgtggt ccgccgcaac atcgaggcgc tgcgcggcaa cgtcgcgatc
     4861 gccagcgagc cgggccgggg caccaccgtc accgtgcggc tgccgctcac gctggccatc
     4921 atcaacggct tccaggtcgg cgtgggcaag tccgtgttcg tcgtgccgat ggacatggtc
     4981 gacgagtgca tcgagttctc cacggagacc ggccaggact acaccgacct gcgcggccag
     5041 gtgctgccgt tcatccgcct gcgggagctg ttcgacgtgc cgggccgccc ggccgtgcgc
     5101 cagaacatcg tcgtcgtcaa gcacgccggc cagaagttcg gcctcgtcgt cgatgccctg
     5161 atgggcgagg cgcaggccgt catcaagccg ctgtcgcgca tgttcgtgca ggtgcgcggc
     5221 atcagcggct ccagcatcct gggcagcggc gacgtcgcgc tgatcctgga tgtgcccgtg
     5281 ctgatgcagc aggcgcagct gtcgcgtggc gcagcggcgc acccggcgtc gtgacgcccc
     5341 cacccgatcg atctctccaa acccagggaa acccaatgct caagaacctc cgtgtcggaa
     5401 ccaagctgtt cggctccttc ctcgtcgtgc tgggcctgct cgcgggcctg ggcgtgttcg
     5461 cgctcgcgaa gctggccaac gtcaatgcgt cggcgctgga aatcgccaac caggcgttgc
     5521 ccagcgtccg agccatcgtc tttgccaacg cgcacatgaa tcgcgtccgc acgctgcaat
     5581 acaaggcggt cgtcgtcgaa ccgcccgaag tcccggccgt cgagaaggag gccgcggcca
     5641 agctcgccga cctcgaacgc gtcaacgccg atttcgccag gctgatcgag aatccggagg
     5701 agcgcgcgct cttcgagagc tacaagtcgg atgtggacgc gttcgtcggc gagaacgcga
     5761 ggatcatgga cctggcgcgc cagaacaagg acgcggaggc gcgggcggtg ctcgaaggcg
     5821 agggccagaa gcactaccag gccatgcgcg ccgcatgcaa caagctggtt gatctggtga
     5881 ccgccgacgg caaccgcatc gccgccgcgg cgcaatcgac ctacgactcg gcgcgcacct
     5941 ggatcatcgc cgcgatcgtc ggctgctcgg cgctggcgct gctcatggcc acgttgatca
     6001 cgcgggggat cacgctgccc gtgcgcgctg cggtggacgc ggcgaaccgc ctggcggagg
     6061 gcgacctcac gca
//
LOCUS       DATCRO010000916         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_977711, whole genome shotgun sequence.
ACCESSION   DATCRO010000916 DATCRO010000000
VERSION     DATCRO010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977711"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1020)
                     /locus_tag="VIN75_24855"
     CDS             complement(118..1020)
                     /locus_tag="VIN75_24855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8977468.1"
                     /translation="MSAKHDIELDSIDSSDLRRRDFVALSVAGAAAATALPAAAIPAG
                     PAVVEKNVTINTPDGSCNAAYFHPAEGRHPAVILWPDAFGLRPSMRDMGRRLAADGYA
                     VLVPNPFYRIGPGPFYETAANVDFKDKTTFAKLGPLMGSIGASGAAEKDAVEFVKFLD
                     AQPEVDKGRRIGTQGYCMGGALVFRTAAAVPERVGAGASFHGGGLVIDKPDSPYLLVP
                     RIKARMLVAIAGSDDARQPDAKDKLRKAFDDAHVAAKVEVFAEQHGWCVPDMPHEDGK
                     AIYNQADAERAWAELLALYKTALG"
     gene            1145..2074
                     /locus_tag="VIN75_24860"
     CDS             1145..2074
                     /locus_tag="VIN75_24860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY8977469.1"
                     /translation="MKTSEADNDVYRTLLESTKAIPWKIDWPTATFEYIGPQIEQLLG
                     WKQDSWVTVNDWAERMHPEDRAWVVDFCVKQSQSGMDHEADYRALGASGEYVWIRDVV
                     HVVRSEAGEPLALIGFMFDISERKKTEEKLLVMQKQLEDYSFRDGLTGVSNRRKFDAV
                     LDEEWTLARRNTQPLSLILIDIDFFKQYNDRHGHVQGDECLKAVATALGTVASRPRDL
                     LARYGGEEFVLVLPATDEAAAQRVSERVRQALFKAQIAHGASSVGQLLTVSQGVGSII
                     PSATDTLTQFLDAVDRRLYRAKQAGRDRSVGAA"
     gene            2076..>2500
                     /locus_tag="VIN75_24865"
     CDS             2076..>2500
                     /locus_tag="VIN75_24865"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8977470.1"
                     /translation="MRIAARLTNRATSHGVTLDTAGKTQPLAVAAKAAGGGSAVNGGE
                     FLMLALATCYCNDLYREAARRGIVLAAVEVEASAEFAAAGLAASDIRYRARVESTAPA
                     AEIEALLRETDQVAEVHNTLRAGLPVRREPWPQAPDAAP"
BASE COUNT          379 a          863 c          898 g          360 t
ORIGIN      
        1 cccgccgttc gttagcgcgc cgaagggtgt tttcgcggca cgcggccatc ggcctggtcg
       61 tcatgccgac gaacggcggg ttacgcctgc ggctaacccg ccctaccaga ccgcgcttca
      121 accgagggcg gtcttgtaca acgccagcag ctcggcccag gcgcgctccg catccgcctg
      181 gttgtagatc gccttgccat cctcgtgcgg catgtccggc acgcaccagc cgtgctgttc
      241 ggcgaagacc tcgaccttgg ccgcgacgtg cgcatcgtcg aacgccttgc gcagcttgtc
      301 cttggcgtcg ggctggcggg cgtcgtcgct gccggcgatg gccaccagca tgcgggcctt
      361 gatcctgggc accagcaggt acggcgaatc gggcttgtcg atgaccaggc caccgccgtg
      421 gaacgaggcg ccggcgccca cgcgctcggg caccgccgcg gccgtgcgga acaccagcgc
      481 gccgcccatg cagtagccct gggtgccgat cctgcggccc ttgtcgacct cgggctgcgc
      541 gtcgaggaac ttgacgaatt cgaccgcgtc cttctcggcc gcgcccgacg ccccgatgga
      601 gcccatcagc ggcccgagct tggcgaaggt cgtcttgtcc ttgaagtcga cgttggcggc
      661 cgtctcgtag aacgggccgg ggccgatgcg gtagaacggg ttgggcacca gcaccgcgta
      721 gccgtcggcg gcgaggcggc ggcccatgtc gcgcatggac gggcgcaggc cgaacgcgtc
      781 cggccacagg atgacggccg gatgcctgcc ctcggccggg tggaagtacg ccgcgttgca
      841 actgccgtcg ggcgtgttga tggtgacgtt cttctcgacc acggcgggcc cggccggtat
      901 ggccgcggcg ggcagggcgg tggccgcggc ggcgccggcc accgacagcg cgacgaagtc
      961 gcggcggcgc aggtcgctgg agtcgatgct gtcgagttcg atgtcgtgct tggcgctcat
     1021 gtccgttgct ctcctcgtga aattcgacct gcttgcaggc gggcaagcgg ccgaggatat
     1081 ctccagggac tgaatccctt cacgcgagcc agttatgctc gtggaaaacc cgcgagagcc
     1141 gcgcatgaag acttccgaag ccgacaacga cgtctaccgg acgctgctcg aatcgaccaa
     1201 ggcgatcccc tggaagatcg actggccgac ggcgacgttc gagtacatcg gcccgcagat
     1261 cgagcagctc ctgggctgga agcaggacag ctgggtcacg gtcaacgact gggccgagcg
     1321 catgcatccg gaggaccgcg cctgggtggt cgacttctgc gtgaagcagt cgcagtccgg
     1381 catggaccac gaggccgact accgcgcgct cggcgcctcg ggcgagtacg tgtggatccg
     1441 cgacgtggtg cacgtggtgc gcagcgaggc gggcgagccg ctggcgctga tcggcttcat
     1501 gttcgacatc agcgagcgca agaagaccga ggaaaagctg ctggtgatgc agaagcagct
     1561 ggaggactac tcgttccgcg acgggctgac gggcgtatcc aaccgccgca agttcgacgc
     1621 ggtgctggac gaggaatgga cgctggcgcg ccgcaacacg cagccgttgt cgctgatcct
     1681 gatcgacatc gacttcttca agcaatacaa cgaccgccac ggccacgtgc agggcgacga
     1741 gtgcctcaag gccgtcgcga cggcgctcgg cacggtggcc agccgcccgc gcgacctgct
     1801 ggcacgctac ggcggcgagg agttcgtgct cgtgctgccc gccacggacg aggcggcggc
     1861 gcagcgcgtc agcgagcgcg tgcgccaggc gctgttcaag gcgcagatcg cgcacggcgc
     1921 gtcgtcggtc ggtcagctgc tgaccgtcag ccagggcgtg ggcagcatca tcccgtcggc
     1981 caccgacacg ctgacgcagt tcctcgacgc cgtcgaccgc cgcctgtacc gcgccaagca
     2041 ggccgggcgc gaccgcagcg tcggcgcggc ctgacatgcg catcgccgcg cgcctgacca
     2101 accgggcgac atcgcacggc gtcacgctcg acaccgccgg caagacgcag ccgctggccg
     2161 tcgcggccaa ggccgcgggc ggcggctctg ccgtcaacgg cggcgagttc ctgatgctgg
     2221 cgctggccac ctgctactgc aacgacctgt accgcgaggc cgccaggcgc gggatcgtgc
     2281 tggcggcggt ggaggtcgag gcctcggccg agttcgcggc cgcgggcctg gcggcctccg
     2341 acatccggta ccgggcgcgg gtcgagtcga ccgccccggc cgccgagatc gaggccctgc
     2401 tgcgcgagac cgaccaggtc gccgaggtgc acaacaccct gcgcgccggc ctgcccgtgc
     2461 ggcgcgagcc gtggccgcag gcgccggacg cggcgccaag
//
LOCUS       DATCRO010000917        10091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_460417, whole genome shotgun sequence.
ACCESSION   DATCRO010000917 DATCRO010000000
VERSION     DATCRO010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460417"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..837)
                     /gene="dnaQ"
                     /locus_tag="VIN75_24870"
     CDS             complement(115..837)
                     /gene="dnaQ"
                     /locus_tag="VIN75_24870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY8977471.1"
                     /translation="MRQIFLDTETTGLSPESGDRMVEIGCIEMDNRRLTGRHLHFYLN
                     PERKNGEGAVAIHGLTDEFLSDKPLFAHVVDEILEFVSGAEIIIHNAAFDVGFLNEEL
                     RRCGRDKFSTHVTGITDTLLMAREMFPGKANSLDALCKRLEVDNSNRTLHGALIDANL
                     LAEVYIRLTRGQDSLVMDMTEDASGSTGDLGGPVDLSVFVLPVLCATESELADHERVL
                     GEIDKASGGKTIWRAQTQKAVA"
     gene            complement(923..1744)
                     /gene="lgt"
                     /locus_tag="VIN75_24875"
     CDS             complement(923..1744)
                     /gene="lgt"
                     /locus_tag="VIN75_24875"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8977472.1"
                     /translation="MALEHPHFNPVALQIGPLAIHWYGLTYMVAFGLFVWLAGRRTTY
                     PWFAREGWVRRDVEDMLFYGVLGVVLGGRLGYVLFYKFDYYLQHLTEIPAVWKGGMSF
                     HGGMLGVIVAMALFAWHRKRPFLQVTDLIAPCVPLGLASGRVGNFINGELWGRPAPDS
                     LPWAMIFPESGSMVPRHPSQIYQFLGEGVLLFIILWIYGRKERGRGQVSGLFLMGYGV
                     LRFAAEFFREPDDFLGLLALGMSMGQWLCVPMVVAGAVMFWWGSRQPRGPAAATA"
     gene            complement(1810..2760)
                     /locus_tag="VIN75_24880"
     CDS             complement(1810..2760)
                     /locus_tag="VIN75_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977473.1"
                     /translation="MADAPFDLRQLRQFTVLAEECHFGRAAARLAMTQPPLTQAIQRL
                     EAALGARLFERSQRRVALSPAGEVLLPLARKLLEGADALAPAVRAAAAGLSGQLRLAF
                     VSSVTYGPLPAWLSGFRAAYPDVALSLREATLDVQLELFAAGEIDAGFVLHAPGAVPP
                     GFEHVRVIDEALVMALPQGHAAWKASAATGSAMPRLRWRDVRAEPLVIFPRTIAPSLF
                     DALVSAYHAGGETLRIAQQAIQMQTIVNLVSGGMGVAWVPESLTHLQRAGVRYARVSG
                     LPGQALRCETSLVWRAGAAPVVERFVEHVRGAPRRSAQAR"
     gene            2880..4565
                     /gene="ilvD"
                     /locus_tag="VIN75_24885"
     CDS             2880..4565
                     /gene="ilvD"
                     /locus_tag="VIN75_24885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8977474.1"
                     /translation="MSQSNRRSRNITEGVARAPNRSMYYAMGYKETDFGKPMIGVANG
                     HSTITPCNSGLQPLADAAVDALKVHGANAQMFGTPTISDGMAMGTEGMKYSLVSREVI
                     SDCVETCVGGQWLDGVIVIGGCDKNMPGGMMGMLRANVPAIYVYGGTIMPGHYKGKDL
                     NIVSVFEAVGQFSAGKMSEEDFCQIERRAIPGSGSCGGMYTANTMSSSFEALGMSLPY
                     SSTMANVEGEKIENTRESARVLVEAVKADLKPRDIVTKKAIENAVAVIMATGGSTNAV
                     LHFLAIAHAAEVEWTIDDFERIRKRTPVLCDLKPSGEFLAIDLHRAGGIPQVMKLLLD
                     AGLLHGDCMTITGKTVAESLADVPSVPRADQKVIRTIANAMYPDGHLAILRGNLATEG
                     CVAKITGLKNPVMTGPARVFDDEQSALAAIMAGRIVAGDVMILRYLGPKGGPGMPEML
                     APTGALIGQGLGESVGLITDGRFSGGTWGMVVGHVAPEAYEGGAIALVHEGDSITIDA
                     HQLLLQLNVADDELARRRAAWKKPAPRYTRGVLAKFANHSSSASFGAVLDKFE"
     gene            4701..5156
                     /locus_tag="VIN75_24890"
     CDS             4701..5156
                     /locus_tag="VIN75_24890"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8977475.1"
                     /translation="MNRFQRLVLALTLACTPWLAHAQGYKCKQADGSVSYQDHACPSG
                     ASSTTVSTDQSLDIGLPPLTGLDASCQQSVHHAVSTCLSPIQDAIKRCYKVRLSSSCN
                     AQLLAGNRAGACAAQAAPCVQDGAIEGRRCIVNVLPPTCVNQLRGAGMR"
     gene            5217..5414
                     /locus_tag="VIN75_24895"
     CDS             5217..5414
                     /locus_tag="VIN75_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HEY8977476.1"
                     /translation="MTIPDPSAAHPGDDPMPLPPPQPDIDLCCGNGCDPCIFDLHDLE
                     MDKYRQALRAWKLRHPQAPAA"
     gene            complement(5425..6402)
                     /locus_tag="VIN75_24900"
     CDS             complement(5425..6402)
                     /locus_tag="VIN75_24900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY8977477.1"
                     /translation="MQSQTKNAKSRDEIATLARHAFGVGLLPGDAGLRELTDGWFNAV
                     HELGLADGRRVVLKIAPPPGAPVMTYERDLMATEVAKMRLVKSDPAIPVPAVLFHDDS
                     LRVCDAPYFFMEKAAGANLDHVRTTLAPDEASAIDRHVGEVVRAINGFRGEWFGLPGH
                     PGLRASTWREAFGRIVESVLQDGARRDVAYPRPADEIRALLHRHGPALDEVREPRLVH
                     WDGWDKNFFVADGRVTGLIDFERALWADPLMEAQFRALSWGGVSDALRGYGRTGFSDT
                     ELVRNRLYTLHLALVMHTECFYRLYPDDQVLNGSRRMLVENLDWLQAHA"
     gene            6474..7250
                     /locus_tag="VIN75_24905"
     CDS             6474..7250
                     /locus_tag="VIN75_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY8977478.1"
                     /translation="MQTPLPLARRDVIAQRLAGGAAVVAATLAQEFDVSEDAIRRDLR
                     ALAAEGRCRRVYGGALPVTPHTQPMFARIDEARGRKEALARRAAESIRPGELLFLDAG
                     STNLALTEFLPEDQELTIATNAIDIAGAVLRRPDLRLIMIGGSVDTTVGGSVDTAAVQ
                     AVAQLNIDRCFLGACAVDAVRGIGAALHADALFKRVLMDATQRCVVLAMNAKLGARAP
                     HRSCALADVDLLVVEHDAPPALTRPLEAAGATLARAAPAA"
     gene            7270..8439
                     /locus_tag="VIN75_24910"
     CDS             7270..8439
                     /locus_tag="VIN75_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977479.1"
                     /translation="MTTLSTVAQPSADRPGARLATRIAFLVAGFGLAAWAPLIPFAKA
                     RLGADDGLMGVLLLCLGAGSVAAMLRTGPLCARYGCKPVVVGSGLALALLLPPLAVAS
                     SPWTLGAVLLAFGGALGSLDVAMNVHAVEVERDARVPLMSGFHAMFSVGGFVGAGFVT
                     ALLSSGVGPLACTVPCVALMVVAMLVAAPRLLEAGRVAAGHSLAWPRSGAMLLLAALA
                     AVCFLVEGAMLDWSALLLTTRSLAAPAQGGLGYAFFAVAMTLGRFSGDAITARIGDRT
                     TMQAGGVLSVLGFVVLLSGGRLEFALAGFALIGLGASNIVPVLFRRCGSMPGTSAAMA
                     VAALTTVGYAGQLMGPAAVGFVSRLASLPAAFWLLAALMAVVPLCANKVLGRPAR"
     gene            8552..9373
                     /locus_tag="VIN75_24915"
     CDS             8552..9373
                     /locus_tag="VIN75_24915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977480.1"
                     /translation="MSNQPNTAMRTFARLRTALAAALLVSASLHAGAAGAPVSGISLL
                     HASAAGYTVDDTTTPAGAYLAVTCDKGACALVPTQVTIANQDVQMHEGLESLPVMHAD
                     LKTHSLFLVQGVPGLAAGPVKTWYVNERFLASDDPAAMAPARRRLDRAVSVDGTDVSF
                     DGHWLQGQDPSCSGPGCGTRQLAWKVRFGETERTLAALWPDGIVGEAGMLGVDDVLIW
                     VGDLDGDGKPDFVVRPQARPDYLELTLFLSTRLQVGKPWRPAARFYHWDPRNAGR"
     gene            complement(9379..>10091)
                     /locus_tag="VIN75_24920"
     CDS             complement(9379..>10091)
                     /locus_tag="VIN75_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8977481.1"
                     /translation="TGDAQPPLVLLHDSLGSVELWRDFPELLARDTGRRVVAYDRAGF
                     GLSDARVTRPSFDFIVEEATTWFPIVRRHLGIDHFAVLGHSVGGGMAVNIAARHSDSC
                     TALVTIAAQAFVEDRTLAGLREARVSFAAPGAMERLARYHGDKAAWVLSAWLDTWLDP
                     AFAHWSLARVLPGVRCPALALHGELDEYGSSAHPAMIARLAGGPARMELLAGLGHVPQ
                     RERPEEIARRVATFLAAA"
BASE COUNT         1464 a         3690 c         3513 g         1424 t
ORIGIN      
        1 gtgcaggttt cgaacctgcg accccgtccg tgtgaaggac gtgctctacc cctgagctaa
       61 ccgcccggca tgtgccggtg ttcgatgtcg ttgcaacatc agaacagcct tggattatgc
      121 cacagctttt tgcgtttgtg cacgccagat cgttttcccg ccgctggcct tgtcgatctc
      181 gcccaacacc cgctcgtggt ccgcgagctc ggactccgtg gcacacagca cgggcagcac
      241 gaagacgctg aggtcgacgg ggccgccgag gtcgccggtg ctgccggatg cgtcctcggt
      301 catgtccatc accagcgaat cctggccgcg cgtgaggcgg atgtagacct cggcgagcag
      361 gttggcgtcg atcagcgcgc cgtgcagcgt gcggttgctg ttgtcgacct ccaggcgctt
      421 gcacagcgcg tcgagcgagt tcgccttgcc ggggaacatc tcgcgcgcca tcagcagcgt
      481 gtcggtgatg cccgtgacgt gcgtgctgaa cttgtcgcgg ccgcagcggc gcagttcctc
      541 gttgaggaag ccgacgtcga aggccgcgtt gtggatgatg atctcggcgc ccgagacgaa
      601 ctcgaggatc tcgtcgacga catgcgcgaa cagcggcttg tcggacagga actcgtcggt
      661 caggccgtgg atggcgacgg cgccctcgcc gttcttgcgc tcgggattga gatagaagtg
      721 caggtgccgg ccggtgaggc ggcggttgtc catctcgatg cagccgatct cgaccatgcg
      781 gtcgccgctc tcgggactca ggccggtggt ttcggtgtcg aggaagatct ggcgcatgga
      841 cgaagggatc gaatcaggct gcgattctcg caggagttcg ggcggccccg gcgtggaatc
      901 cgcccgataa acgacgcctg cgtcaggcgg tggcggcggc cgggccgcgc ggctggcgcg
      961 agccccacca gaacatgacg gcgccggcga cgaccatcgg cacgcacagc cactggccca
     1021 tgctcatgcc cagcgccagc aggcccagga agtcgtcggg ctcgcggaag aactcggcgg
     1081 cgaaacgcag cacgccgtag cccatgagga acaggccgga gacctggccg cggccgcgct
     1141 ccttcctgcc gtagatccac aggatgatga acagcagcac gccctcgccc aggaactggt
     1201 agatctgcga cgggtggcgc ggcaccatcg agcccgactc cgggaagatc atcgcccacg
     1261 gcagcgagtc gggggccggc cggccccaca gttcgccgtt gatgaagttg cccacgcgac
     1321 cgctcgccag gcccagcggc acgcacgggg cgatgaggtc ggtgacctgc aggaacgggc
     1381 gcttgcggtg ccacgcgaac agcgccatcg cgacgatcac gcccagcatg ccgccgtgga
     1441 aggacatgcc gcccttccag acggccggga tctcggtcag gtgctgcagg tagtagtcga
     1501 acttgtagaa caggacgtag ccgagccgtc cgcccagcac cacgcccagc acgccgtaga
     1561 acagcatgtc ctcgacgtcg cgccgcaccc agccctcgcg cgcgaaccac ggataggtgg
     1621 tgcggcggcc ggcgagccac acgaacaggc cgaacgcgac catgtacgtg aggccgtacc
     1681 aatggatcgc gagcgggccg atctgcaggg ccacgggatt gaagtgcgga tgctccagcg
     1741 ccatcgagac aaactccttg aacagcgggc gattgtcgcc gtttcgcgtg acgcttcctg
     1801 acggcgccgt cagcgagcct gcgccgagcg ccgcggcgcg ccgcgcacgt gctcgacgaa
     1861 gcgctccacc acgggcgccg cgcctgcgcg ccacacgagg ctggtctcgc agcgcagcgc
     1921 ctggccgggc agcccgctca cgcgcgcgta acgcacgccc gcgcgctgca ggtgcgtgag
     1981 cgactccggc acccaggcca cgcccatgcc gcccgacacg aggttgacga tcgtctgcat
     2041 ctggatcgcc tgctgcgcga tgcgcagcgt ctcgccgccc gcgtggtagg ccgacaccag
     2101 cgcgtcgaac agcgacggcg cgatggtgcg cgggaagatc accagcggct cggcgcggac
     2161 gtcgcgccag cgcagcctcg gcatggccga acccgtcgcc gccgacgcct tccaggctgc
     2221 gtggccctgc ggcagcgcca tcacgagcgc ctcgtcgatc acgcgcacgt gctcgaagcc
     2281 gggcggcacc gcgccgggcg catgcagcac gaagccggcg tcgatctcgc cggcggcaaa
     2341 cagctccagc tgcacgtcca gcgtggcctc gcgcaggctc agcgcgacat cgggataggc
     2401 cgcgcggaag ccgctgagcc aggccggcag cgggccgtac gtgacgctgg agacgaacgc
     2461 cagccgcagc tggcccgaca gccccgccgc ggccgcgcgc accgcgggcg cgagcgcgtc
     2521 ggcgccctcg agcagcttgc gcgcgagcgg cagcagcacc tcgcccgccg ggctcagcgc
     2581 gacgcggcgc tgactgcgct cgaacagccg ggcgccgagc gcggcctcca gccgctggat
     2641 cgcctgcgtc agcggcggct gcgtcatggc caggcgcgcg gcggcgcggc cgaaatggca
     2701 ctcctcggcg agcacggtga actggcgcag ctggcgcagg tcgaacgggg cgtcagccat
     2761 ggcggatcat tgattcacaa gacatatcaa tctggcgcga tgaatctatt ggacacgcat
     2821 tttgccccgc ccgatactgc gccctcaccc agcatcccgc cagactccag gagaccgcca
     2881 tgtcccagtc caatcgccgt tccaggaaca tcaccgaggg cgtcgcccgt gcccccaacc
     2941 gctcgatgta ctacgcgatg ggctacaagg agacggactt cggcaagccg atgatcggcg
     3001 tggccaacgg ccattcgacg atcacgccct gcaactcggg cctgcagccg ctggccgacg
     3061 ccgcggtcga tgcgctcaag gtccatggcg ccaatgcgca gatgttcggc acgcccacca
     3121 tctccgacgg catggccatg ggcaccgagg gcatgaagta cagcctggtc tcgcgcgagg
     3181 tgatctccga ctgcgtcgag acctgcgtcg gcggccagtg gctggacggc gtgatcgtca
     3241 tcggcggctg cgacaagaac atgccaggcg gcatgatggg catgctgcgc gccaacgtgc
     3301 cggccatcta cgtctacggc ggcacgatca tgccgggcca ctacaagggc aaggacctca
     3361 acatcgtcag cgtgttcgag gccgtgggcc agttctccgc cggcaagatg agcgaggagg
     3421 acttctgcca gatcgagcgc cgcgccatcc cgggctccgg ctcgtgcggc ggcatgtaca
     3481 ccgccaacac gatgtcgtcg tcgttcgagg cgctgggcat gtcgctgccc tactcgtcga
     3541 cgatggccaa cgtcgagggc gagaagatcg agaacacgcg cgaatcggcg cgcgtgctgg
     3601 tcgaggccgt caaggccgac ctgaagccgc gcgacatcgt cacgaagaag gcgatcgaga
     3661 acgccgtggc cgtcatcatg gccaccggcg gctccaccaa cgccgtgctg cacttcctgg
     3721 ccatcgcgca cgcggccgag gtcgagtgga cgatcgacga cttcgagcgc atccgcaagc
     3781 gcacgccggt gctgtgcgac ctcaagccca gcggcgagtt cctggccatc gacctgcacc
     3841 gcgccggcgg catcccgcag gtcatgaagc tgctgctgga cgccggcctg ctgcacggcg
     3901 actgcatgac catcaccggc aagacggtgg ccgaatcgct ggccgacgtg ccgtccgtgc
     3961 cgcgcgccga ccagaaggtg atccgcacca tcgccaacgc gatgtacccg gacggccacc
     4021 tggccatcct gcgcgggaac ctcgccaccg agggctgcgt cgccaagatc accggcctga
     4081 agaaccccgt gatgaccggc ccggcccgcg tgttcgacga cgagcagtcg gccctggccg
     4141 ccatcatggc cggcaggatc gtcgccggcg acgtgatgat cctgcgctac ctgggcccca
     4201 agggcggccc gggcatgccc gagatgctgg cgcccacggg cgcgctgatc ggccaggggc
     4261 tgggcgaatc ggtgggcctg atcaccgacg ggcgcttctc cggcggcacc tggggcatgg
     4321 tggtgggcca cgtcgcgccg gaagcctacg agggcggcgc gatcgcgctg gtgcacgagg
     4381 gcgactcgat cacgatcgac gcccaccaat tgctgctgca gctcaacgtc gccgacgacg
     4441 agctcgccag gcgccgcgcc gcctggaaga agccggcgcc gcgctacacc cgcggcgtgc
     4501 tggccaagtt cgccaatcac tcgtccagcg cgagcttcgg cgcggtgctc gacaagttcg
     4561 agtgacccgg cttcgtggca ggtttgccaa gaaaccgcaa cattccggaa atccgccacg
     4621 gatccccgcc ggcgcggcgt cgccgctcga aggacgtgag gtagcctgcg cctcggatca
     4681 atacccgggg agggataccc atgaaccgtt tccagcgcct ggtgctggcc ctgacgctgg
     4741 cgtgcacgcc gtggctcgcg cacgcgcagg gctacaagtg caagcaggcc gacggctcag
     4801 tcagctacca ggaccacgcc tgcccgtcgg gcgccagcag cacgacggtg tcgaccgacc
     4861 agagcctgga catcggcctg ccgccgctga ccggcctgga cgcatcctgc cagcagagcg
     4921 tgcaccacgc cgtgtcgacc tgcctctcgc cgatccagga cgcgatcaag cgctgctaca
     4981 aggtccgcct gtcctcctcg tgcaacgccc agttgctcgc cggcaaccgc gccggggcct
     5041 gcgccgcgca ggccgccccc tgcgtgcagg acggcgcgat cgagggccgg cgctgcatcg
     5101 tcaacgtgct gccgcccacc tgcgtgaacc agctgcgcgg cgccggcatg cgctgacgcg
     5161 gccgcgcgac gacaccgggg cgcctgcggg cgcctttttc tttggacaat gcttgcatga
     5221 cgatccccga cccgtccgcc gcgcaccccg gcgacgaccc gatgccgctg ccgccgccgc
     5281 agcccgacat cgacctgtgc tgcggcaacg gctgcgaccc ttgcatcttc gacctgcacg
     5341 acctcgagat ggacaagtac cgccaggccc tgcgcgcctg gaagctgcgc cacccgcaag
     5401 cgcccgcggc ctgagcggca gcgctcaggc gtgcgcctgc agccagtcca ggttctcgac
     5461 cagcatccgg cgcgagccgt tcagcacctg gtcgtccggg tacagccggt agaagcactc
     5521 ggtgtgcatc accagcgcca ggtgcagcgt gtagaggcgg ttgcgcacca gttcggtgtc
     5581 gctgaacccg gtgcggccgt agccgcgcag ggcgtcggag acgccgcccc aggacagcgc
     5641 gcggaactgc gcctccatca gcgggtcggc ccagagcgcg cgctcgaagt cgatcaggcc
     5701 cgtgacgcgg ccatcggcga cgaagaagtt cttgtcccag ccgtcccaat gcaccaggcg
     5761 gggctcgcgc acctcgtcca gcgccgggcc gtggcgatgc agcagcgcgc ggatctcgtc
     5821 ggccggacgg gggtacgcca cgtcgcggcg cgctccgtcc tgcagcaccg attcgacgat
     5881 ccgcccgaac gcctcgcgcc acgtggacgc gcgcagcccg gggtgccccg gcaggccgaa
     5941 ccactcgccg cgaaagccgt tgatggcgcg cacgacctcg ccgacatggc ggtcgatggc
     6001 gctcgcctcg tcgggcgcca gggtcgtgcg gacgtggtcg agattggcgc cggccgcctt
     6061 ctccatgaag aagtacggcg cgtcgcagac gcggagcgag tcgtcgtgga acagcacggc
     6121 ggggaccggg atcgccgggt cggacttcac cagccgcatc ttggcgacct cggtggccat
     6181 caggtcgcgc tcgtaggtca tcaccggagc gccgggcggc ggcgcgatct tcaggaccac
     6241 gcggcggccg tcggccagcc cgagctcgtg gacggcattg aaccagccgt cggtcagctc
     6301 gcgcaggccg gcgtcgccgg ggagcaggcc gacgccgaag gcgtggcggg ccaacgtggc
     6361 gatctcgtcg cgcgacttcg cgttcttggt ctggctctgc acggtgtccc cgctcctggt
     6421 ttgcacgatt gtacgtgttt gcattcttgt ttgtgcacgt ttatcatggc cggatgcaga
     6481 cgcccctccc gctcgcccga cgcgacgtca tcgcccagcg cctggccggc ggcgccgccg
     6541 tggtggccgc cacgctggcg caggaattcg acgtctcgga ggacgcgatc cggcgcgacc
     6601 tgcgcgcgct cgccgcggag ggccgttgcc gccgcgtgta cgggggcgcc ctgcccgtca
     6661 cgccgcacac ccagccgatg ttcgcccgca tcgacgaggc gcgcggccgc aaggaggcgc
     6721 tggcgcgccg ggccgccgag tcgatccggc ccggcgagct gctgttcctg gacgccggca
     6781 gcaccaacct cgcgctgacc gagttcctgc ccgaagacca ggaactcacg atcgccacca
     6841 acgcgatcga catcgccggc gccgtgctgc gccggcccga cctgcgcctg atcatgatcg
     6901 gcggcagcgt cgacaccacc gtcggcggca gcgtcgacac cgccgccgtg caggccgtcg
     6961 cccagctcaa catcgaccgc tgcttcctgg gcgcctgcgc ggtggacgcc gtgcgtggca
     7021 tcggcgcggc tctgcacgcc gacgcgctgt tcaagcgcgt gctgatggac gcgacccagc
     7081 gctgcgtcgt cctcgcgatg aacgccaagc tcggcgcgcg cgcgccgcac cgcagctgcg
     7141 cgctggccga cgtcgacctg ctcgtggtgg agcatgacgc cccgcccgcc ctcacgcgcc
     7201 ccctcgaagc cgccggcgcc acgctggccc gggccgcacc cgcggcctga ccctcttttt
     7261 cgacccgaga tgacaacgct atccaccgtc gcgcaaccct ccgccgaccg ccccggcgcc
     7321 cgcctggcca cccgcatcgc cttcctcgtg gccggcttcg gcctggccgc ctgggcgccg
     7381 ctgatcccgt ttgcgaaagc gcggctcggc gccgacgacg gcctgatggg cgtgctgctg
     7441 ctgtgcctgg gcgcgggctc cgtcgccgcg atgctgcgca ccgggccgct gtgcgcgcgc
     7501 tacggctgca agcccgtcgt cgtcggcagc ggcctcgcgc tggcgctgct gctgccgccg
     7561 ctggccgtgg cgagctcgcc gtggacgctg ggcgccgtgc tgctcgcgtt cggcggcgcg
     7621 ctcggctcgc tggacgtcgc gatgaacgtg cacgcggtgg aggtcgagcg cgacgcgcgc
     7681 gtgccgctga tgtcgggctt ccacgcgatg ttcagcgtcg gcggcttcgt cggcgccggg
     7741 ttcgtcacgg cgctgctgtc gtcgggcgtc gggccgctcg cgtgcaccgt gccgtgcgtc
     7801 gcgctgatgg tcgtcgcgat gctcgtggcc gcgcctcggc tcctcgaggc cggccgcgtg
     7861 gccgccggcc actcgctcgc gtggcctcgc agcggcgcga tgctgctgct ggccgcgttg
     7921 gcggccgtgt gcttcctcgt cgagggcgcg atgctggact ggagcgcgct gctgctgacg
     7981 acgcgctcgc tcgcggcgcc cgcgcagggc ggcctgggct acgccttctt cgcggtggcg
     8041 atgacgctgg gacgcttcag cggcgacgcc atcaccgcgc gcatcggcga ccgcacgacc
     8101 atgcaggccg gcggcgtgct gtcggtgctg ggcttcgtcg tcctgctgtc cggcggccgg
     8161 ctcgagttcg cgctggccgg tttcgcgctg atcgggctcg gcgcctccaa catcgtgccg
     8221 gtgctgttcc ggcgctgcgg cagcatgccg ggcacatcgg cggcgatggc cgtggccgcg
     8281 ctcaccaccg tcggctacgc gggccagctg atgggcccgg cggcggtcgg cttcgtctcg
     8341 cgcctggcca gcctgcccgc ggcgttctgg ctgctcgcgg cgctgatggc cgtggtgccg
     8401 ctgtgcgcga acaaggtgct gggacggccc gcgcgctgac gcgcgggcgc atgcgcggct
     8461 ggaccgcgtg agcaaagtct ccgcgcgccg ccggggtttg ctcgccgcgc cgcccatgat
     8521 cgttcgtgca cggatgccta cgctgccagg catgtccaac caaccgaaca ccgccatgcg
     8581 cacgttcgcc cgccttcgca ccgccctcgc cgccgccctg ctcgtctccg cgtccctgca
     8641 cgcgggtgcg gccggcgcac cggtctccgg gatctcgctg ctgcacgcga gcgcggcggg
     8701 ctacaccgtc gacgacacca ccaccccggc gggcgcctac ctggccgtca cctgcgacaa
     8761 gggcgcctgc gcgctggtgc ccacgcaggt caccatcgcc aaccaggacg tgcagatgca
     8821 cgaggggctc gagtcgctgc ccgtgatgca cgccgacctc aagacgcact cgctgttcct
     8881 cgtgcagggc gtgccgggcc tggcggccgg ccccgtcaag acctggtacg tcaacgagcg
     8941 cttcctggcc tcggacgacc cggccgcgat ggcgcccgcg cgccgccgcc tcgatcgcgc
     9001 ggtgtccgtc gacggcaccg acgtctcgtt cgacggccac tggctgcagg gccaggatcc
     9061 gtcgtgcagc ggcccgggct gcggcacgcg ccagctcgcg tggaaggtgc gcttcggcga
     9121 aaccgagcgc acgctggccg cgctgtggcc cgacggcatc gtcggcgagg ccggcatgct
     9181 gggcgtggac gacgtgctga tctgggtcgg cgacctcgac ggcgacggca agcccgactt
     9241 cgtcgtccgc ccgcaggcgc gcccggacta cctcgagctg acgttgttcc tgtcgacgcg
     9301 gctgcaggtg ggcaagccgt ggcggccggc cgcccgcttc taccactggg atccgcgcaa
     9361 cgcgggtcgc tgaacgactc aggcggccgc gaggaacgtc gcgacccggc gcgcgatctc
     9421 ctcgggccgc tcgcgctggg gcacgtggcc cagccccgcg agcagttcca tgcgcgccgg
     9481 gccgccggcc agccgcgcga tcatggcggg gtgggccgag ctgccgtatt cgtcgagctc
     9541 gccgtgcagc gccagcgcag ggcagcgcac gccgggcagc acgcgcgcca gcgaccagtg
     9601 cgcgaacgcc ggatcgagcc aggtgtcgag ccaggccgac agcacccacg ccgccttgtc
     9661 gccgtggtag cgcgccagcc gctccatcgc gccgggggcc gcgaacgata cccgcgcctc
     9721 gcgcagccct gccagcgtgc gatcctcgac gaaggcctgc gccgcgatcg tcacgagcgc
     9781 cgtgcagctg tccgaatgcc gcgccgcgat gttgacggcc atgccgccgc ccacgctgtg
     9841 gcccagcacg gcgaagtggt cgatgcccag gtgcctgcgc acgatcggga accaggtcgt
     9901 cgcctcctcg acgatgaagt cgaacgacgg ccgcgtgacg cgcgcgtccg acagcccgaa
     9961 gcccgcgcgg tcgtaggcga cgacgcgccg gccggtgtcg cgcgcgagca gttcgggaaa
    10021 gtcgcgccac agctcgacgc tgcccagcga gtcgtgcagc agcaccagcg gcggctgcgc
    10081 gtcgccagtg c
//
LOCUS       DATCRO010000918         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_134318, whole genome shotgun sequence.
ACCESSION   DATCRO010000918 DATCRO010000000
VERSION     DATCRO010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134318"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VIN75_24925"
     CDS             complement(<1..550)
                     /locus_tag="VIN75_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977482.1"
                     /translation="MKRSLLNTASAILLLTGSMAGCDSVPEAPGARGLGAAAQAPAER
                     AVPDSPIVPVAGKPVLWGYVDGAGIAHFASSQVDGRYEQVLRETEGRRVVGKTQGTDG
                     LLTWLEISPDVKALQPVLDEAAQAPAERAVPSAPIVPVAGKPVLWGYVDGAGVAHFAS
                     SQIDGRYEQVLRETDGRRVAGKT"
     gene            complement(547..1116)
                     /locus_tag="VIN75_24930"
     CDS             complement(547..1116)
                     /locus_tag="VIN75_24930"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8977483.1"
                     /translation="MPTSEIAESAHLRIRPTMLSDLDFVVSVEQDARNLVFITPWERT
                     QHEGAIRFPDSRHFIVEAGDQWEPIGFVILQGCRNPNRSVELKRIVLQTQGRGLGRAC
                     VRMLKRLAFRDLAAHRFWLDVKSLNARALRLYASEGFVEEGRLRESVRVGTGETAGWE
                     SLVVMSLLDREYQARVDAGLETAFSGQGA"
     gene            complement(2133..>3937)
                     /locus_tag="VIN75_24935"
     CDS             complement(2133..>3937)
                     /locus_tag="VIN75_24935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY8977484.1"
                     /translation="VTLHADGSVTIDDDGRGIPFGLHPEGQVPVIEIVYTRLHAGGKF
                     DKGSGGAYSFSGGLHGVGVSVTNALAKKLEVAVFRDGQFAALTFSGGDVTVPLEVRKL
                     TGGERKHGTRVTVWPDPKYFESAELPRGELVHLLRSKAVLMPGVTVTLTIEKAGKEPE
                     VLTWSYQGGLRDYLQQTLTAEPIVPLFEGERFAGTNESENFAEGEGANWCVAFTDDGA
                     AVRESYVNLIPTVAGGTHESGLKDGLFTAVKSFIEMHGLLPKGVKLMPEDVFSRASFV
                     LSAKVLDPQFQGQTKERLNSRDALRLVSAFTKPALELWLNQHVEYGRKLAELVIRQAQ
                     ARQKASQKVEKRKGSGVAVLPGKLTDCESRDLTLNEVFLVEGDSAGGSAKMGRDKETQ
                     AILPLRGKVLNTWEVERDRLFANNEIHDIAVAIGVDPHGPNDSPDMAGLRYGKICILS
                     DADVDGSHIQVLLLTLFFRHFPQLVARGHVYVAKPPLYRVDAPARGRKPAAKIYALDD
                     GELEATLDKLRKEGARDDSWKISRFKGLGEMNAEHLWETTLNPETRRLLRVELGQLDL
                     LQTEGLFTRLMGKGEAHARRELMELRGDAVEIDI"
BASE COUNT          573 a         1386 c         1352 g          626 t
ORIGIN      
        1 gcgtcttgcc cgccacgcgc cggccgtcgg tctcgcgaag cacttgttca tagcgcccgt
       61 cgatctggct gctggcgaaa tgggccacgc cggcgccgtc gacgtagccc cacagcaccg
      121 gcttgccggc gacgggaacg atcggggcgc tgggaacagc gcgttcggcc ggggcctgcg
      181 ccgcctcgtc gagcacgggc tgcagcgcct tgacgtcggg cgagatctcc agccacgtca
      241 gcaggccgtc ggtgccctgc gtcttgccga ccacgcgccg gccctcggtc tcgcgcagca
      301 cctgctcgta gcggccgtcg acctggctgc tggcgaaatg cgcgatgccg gcgccgtcga
      361 cgtagcccca cagcaccggt ttgccggcca cgggaacgat cgggctgtcg ggcaccgcgc
      421 gctcggccgg cgcctgcgcg gccgcgccga gcccgcgggc gccgggcgcc tcgggcaccg
      481 agtcgcagcc ggccatcgac cccgtcaaca acaggatcgc gctggccgtg ttgagcagcg
      541 agcgcttcat gcgccttgcc ccgagaaggc cgtctcgagc ccggcgtcga cgcgcgcctg
      601 gtactcgcga tcgagcagcg acatcacgac caggctttcc cagcccgcgg tctcgccggt
      661 gcccacgcgc acgctctcgc gcagccggcc ttcctcgacg aagccctcgc tggcgtacag
      721 ccgcagggcg cgcgcattga gcgacttcac gtccagccag aagcggtgcg cggccaggtc
      781 gcggaacgcc agccgcttga gcatgcgcac gcaggcgcgc ccgaggccac ggccctgggt
      841 ctggagcacg atgcgcttga gctcgaccga gcggttcggg ttgcggcagc cctgcaggat
      901 cacgaagccg atcggctccc actggtcacc ggcctcgacg atgaagtgcc gcgagtccgg
      961 aaagcggatc gcgccctcgt gctgcgtgcg ctcccacggc gtgatgaaca cgaggttgcg
     1021 cgcgtcctgc tccaccgaca cgacgaagtc gaggtcggac agcatcgtcg gacggatccg
     1081 cagatgggcg ctctcggcaa tctcgctggt cggcatcgga aagggacgga tcgggaatgg
     1141 caggttggca cgaccggcag cgcgacggcc gaggcatgct cgaccactgg ccgggcaacg
     1201 gagcgtggat tatggaaagg atcgccgaag cgcgtgccgg gcaatccccg atgaacgcgg
     1261 gatttctgca cgttttggca tcctgactgg ttaagtaacg cttccgcgcg cgaggttccc
     1321 gccaccgaat gggtggttat gtaagcggtt gaatcgaggc gaaccgatgc gcaagatgac
     1381 gggcttggcc gctcgaccgg agatcgcggc ggttcgccgc cggatcgagc gcttgcggcg
     1441 cggccgtgcc agtttcggca agcaagcgac catgtcgttg cgtaggcggg tacgcattcg
     1501 ggacacgttg gacaggcgga tgtcgcgagg cgatgcgccg agggtctgcg cctgaatggc
     1561 gcgtgccggg gtctgcacga tccggacgcc cctcgtccga caggcgtggc gcgccggcag
     1621 accgaggtgt ctgggctcga acgacgcacg ccgagctgtc ggcgacgcag cccccgccga
     1681 tcgtgcgtcg tgccagacct gacaccgaac gtgcgccatg tcttccagcg atcggtgtca
     1741 gcgatcggtg tcaggtctcg catgttgcac gtccggcact tgcgccattg gccggcagac
     1801 cggcgtgcaa cattcaagac cagacacctc ggtgcggtcg cacgccgact cttgtaagcc
     1861 caagcgcgat cgagcgcgga cgcgatcgac gccacgctgg cgcgcaggcg ggcacagcgc
     1921 ctctctcctt cactgccgtg cgcgcatgac cgacacgcgc ggtgcgcact tgcccatgtg
     1981 tggacgtcca cgtgtacacc ccatgcgtac ccgcgcacat gccccgcgca tgccccgcgc
     2041 acgtgcgccc gctcgcgcgc tcgcgtcacg ccggcgagcg agcgcccccc gcccgcgtcg
     2101 cgcgtgcgcg cagccgcgac tcacaggggg ggtcagatgt cgatctcgac cgcgtccccg
     2161 cgcagctcca tcagctcgcg ccgtgcatgc gcttcgccct tgcccatcag gcgcgtgaac
     2221 aggccctcgg tctgcagcag gtcgagctgg cccagttcga ctcgcaagag gcggcgcgtc
     2281 tccgggttca gcgtggtctc ccacaggtgc tcggcgttca tctcgccaag gcccttgaaa
     2341 cggctgatct tccaggaatc gtcgcgcgcg ccttccttgc gcagcttgtc cagggtggcc
     2401 tcgagttcgc cgtcgtcgag cgcgtagatc ttggcggccg gcttgcgacc gcgcgccggc
     2461 gcatcgacgc gatacagcgg cggcttggcc acatacacgt ggccgcgcgc caccagttgc
     2521 gggaagtgcc ggaagaacag cgtcagcagc agcacctgga tgtgcgagcc gtcgacgtcc
     2581 gcgtccgaca ggatgcagat cttgccgtag cgcaggcccg ccatgtcggg tgagtcgttc
     2641 gggccatgcg gatcgacgcc gatcgccacc gcgatgtcgt ggatctcgtt gttggcgaac
     2701 aggcggtcgc gctcgacttc ccacgtgttg agcaccttgc cgcgcaacgg caggatggcc
     2761 tgcgtttcct tgtcgcggcc catcttggcc gagccgccgg ccgagtcgcc ctcgaccagg
     2821 aagacttcgt tgagggtcag gtcgcggctc tcgcagtcgg tgagcttgcc tgggagcacg
     2881 gcgacgccgg agcccttgcg cttctccacc ttctggctgg ccttctggcg cgcctgcgcc
     2941 tggcggatga ccagctcggc cagcttgcgt ccgtactcga cgtgctggtt gagccacagc
     3001 tccagcgccg gcttggtgaa cgcggatacc agccgcagcg cgtcgcggct gttcaggcgc
     3061 tccttggtct ggccctggaa ctgcgggtcg agcaccttgg ccgacagcac gaagctggca
     3121 cgtgagaaca cgtcctcggg catcagcttg acgcccttgg gcaggaggcc gtgcatctcg
     3181 atgaagctct tgaccgccgt gaacaggccg tccttcaggc cggactcgtg cgtgccgccg
     3241 gccaccgtcg ggatgaggtt gacgtagctc tcgcgcacgg cggcgccatc gtcggtgaag
     3301 gcgacgcacc agttcgcgcc ctcgccctcg gcgaagttct ccgactcgtt cgtgcccgcg
     3361 aagcgctcgc cctcgaacag cggcacgatg ggctcggccg tgagcgtctg ctgaaggtag
     3421 tcgcgcaggc cgccctggta ggaccaggtg aggacctcgg gctccttgcc cgccttctcg
     3481 atggtgagcg tgacggtgac gcccggcatc agcaccgcct tgctgcgcag caggtgcacc
     3541 agctcgccgc gcggcagctc cgcgctctcg aagtacttcg ggtccggcca cacggtgacg
     3601 cgcgtgccgt gcttgcgctc gccgcccgtc agcttgcgca cctccagcgg cacggtgacg
     3661 tcgccgccgg agaacgtgag cgcggcgaac tggccgtcgc ggaacaccgc gacctccagc
     3721 ttcttggcca gcgcgttggt gacgctgacg cccacgccgt gcaggccgcc ggagaagctg
     3781 tacgccccgc cgctgccctt gtcgaacttg ccgcccgcgt gcaggcgcgt gtagacgatc
     3841 tcgatgaccg gcacctgccc ttcagggtgc aggccgaacg ggatgccgcg gccgtcgtcg
     3901 tcgatcgtga ccgagccgtc ggcatgcagg gtgaccg
//
LOCUS       DATCRO010000919         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_888062, whole genome shotgun sequence.
ACCESSION   DATCRO010000919 DATCRO010000000
VERSION     DATCRO010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888062"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VIN75_24940"
     CDS             <1..823
                     /locus_tag="VIN75_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8977485.1"
                     /translation="GRFKLAKLNSDEQPEIAGQLSQAFGVRSIPFCVMFHQGQPVDGF
                     VGALPEGEIRAFLDKHVPGADELAAEGDAQAAEALLAEGDTDAALARLQEAVAIDPAN
                     ESARYDYLRLLIETSRSNPELLVQARQAYQPVAQRVLPNAKFDAIGFWLDACEKAAGA
                     RSHDQLAAAIAANKRDFDARFELAQSYLATGAFTQALDELLEILMRDKSWNDELARKV
                     YVGILELMTKPAPKPVAEKAAGDQPKLELTGHATAVGADPVIDQYRRRLSMTVLS"
     gene            complement(953..2047)
                     /locus_tag="VIN75_24945"
     CDS             complement(953..2047)
                     /locus_tag="VIN75_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977486.1"
                     /translation="MSRLLERLDAQIAQAEEVFDRECLKSRRAAVLARHGQFSEARFA
                     LQGLRSQSQRLRNPVLSAWVAFVDGLIEHCESLSPSARAKFQRAYDLASTAGDAELHS
                     LCAAWLAIADFNANDIDATVAHAREALRLAPPTSHAARARAALVVASAWRVAGDDERA
                     IPWYKVARQHAATEGDVSLVSVLLHNMAAFQVGRISLEGAFDRADLADAQRVLLEAES
                     TGNYDAGVGNGQLMAEVPLLRAQLMTVLGQYDAAISLFDAQLPRARAEGQVHREARFL
                     ADALFAEVKLGRLDEAVKRLRAVNAALPLMTEHDDIAATHARLAVATRALGKGDQADA
                     HAAQAEAALEKHRAEQRRWADALAAVDFET"
     gene            complement(2089..>2480)
                     /locus_tag="VIN75_24950"
     CDS             complement(2089..>2480)
                     /locus_tag="VIN75_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8977487.1"
                     /translation="VASAMIGNELANHVRDVSIALYERAAEYALTKGIIIADTKFEFG
                     LDADGTLTLMDEVLTPDSSRYWPVDGYAEGSNPPSYDKQFVRDWLESVRIDGQPWNKK
                     APAPAMPAEVGERTAAKYREAFERLAG"
BASE COUNT          353 a          900 c          886 g          341 t
ORIGIN      
        1 gggccgcttc aagctcgcca agctcaacag cgacgagcag cccgagatcg ccggccagct
       61 gagccaggcc ttcggcgtgc gctccatccc gttctgcgtg atgttccacc agggccagcc
      121 ggtcgacggc ttcgtcggcg ccctgcccga gggcgagatc cgcgctttcc tcgacaagca
      181 cgtgcccggc gccgacgaac tcgcggccga aggcgacgcg caggcggccg aggcgctgct
      241 ggccgagggc gacaccgacg ccgcgctcgc ccgcctgcag gaggccgtgg cgatcgaccc
      301 ggccaacgaa tcggcccgct acgactacct gcgcctgctg atcgagacga gccgcagcaa
      361 ccccgaattg ctcgtgcagg cgcgccaggc ctaccagccg gtggcccagc gcgtgctgcc
      421 caacgcgaag ttcgacgcca tcggcttctg gctggacgcc tgcgagaagg ccgccggcgc
      481 acgatcgcac gaccagctcg ccgcggccat cgccgccaac aagcgcgact tcgacgcccg
      541 cttcgagctg gcccagtcgt acctggccac cggcgcgttc acgcaggcgc tggacgagct
      601 gctggagatc ctgatgcgcg acaagtcgtg gaacgacgag ctcgcgcgca aggtctacgt
      661 cggcatcctc gagctgatga ccaagccggc ccccaagccc gtcgcggaga aggccgcggg
      721 cgaccagccc aagctggagc tgacggggca cgcgacggcg gtgggcgcgg atccggtgat
      781 cgaccagtac cgccggcgct tgagcatgac ggtgctgagc tgacaggtcg aattcgtagg
      841 gcgggatagc cgcaggcgta acccgccatg gtggcgtctc gcaacgccgg cgaacggcgg
      901 gttacgcctt cggctaaccc gccctaccaa tacgccgcgc gtcgcggggg aatcaggtct
      961 cgaaatcgac cgccgccagc gcgtccgccc agcggcgctg ctccgcgcgg tgcttctcca
     1021 gcgccgcctc ggcctgggcc gcgtgcgcgt cggcctggtc gcccttgccg agcgcgcgcg
     1081 tggccaccgc caggcgcgcg tgggtggcgg cgatgtcgtc gtgctcggtc atcagcggca
     1141 gcgccgcgtt gacggcgcgc aggcgcttga ccgcctcgtc cagccggccc agcttgacct
     1201 cggcgaacag cgcgtcggcc aggaagcgcg cctcgcggtg cacctggccc tcggcgcggg
     1261 cgcgcggcag ctgcgcgtcg aacagcgaga tggcggcgtc gtactggccc agcaccgtca
     1321 tcagctgcgc gcgcagcagc ggcacctcgg ccatcagctg gccgttgccc acgcccgcgt
     1381 cgtagttgcc ggtggactcg gcctccagca gcacgcgctg ggcgtcggcc aggtcggcgc
     1441 ggtcgaacgc gccctccagg ctgattcggc cgacctggaa cgcggccatg ttgtgcagca
     1501 gcacgctcac gagcgagacg tcgccctccg tggccgcatg ctggcgcgcg accttgtacc
     1561 aggggatggc gcgctcgtcg tcgccggcca cgcgccacgc gctggccacc acgagcgcgg
     1621 cgcgcgcgcg ggcggcgtgc gacgtcggcg gcgcgaggcg cagcgcctcc ctggcgtggg
     1681 cgacggtggc gtcgatgtcg ttggcgttga agtcggcgat cgccagccac gcggcgcaca
     1741 gcgagtgcag ctcggcgtcg cccgcggtgg acgcgaggtc gtaggcgcgc tggaacttgg
     1801 cgcgcgccga cggcgacagc gactcgcaat gctcgatcag cccgtcgacg aacgcgaccc
     1861 aggccgacag caccgggttg cgcagccgct ggctctgcga gcgcagcccc tgcagcgcga
     1921 agcgcgcctc ggagaactgc ccgtggcgcg ccagcaccgc cgcgcgcctg gacttcaggc
     1981 actcgcgatc gaagacctcc tcggcctggg cgatctgcgc gtcgagacgt tcgagcaggc
     2041 gggacatgtg ctggagcaac ggagtgggca agaccggcgc gcgtcgagtc agcccgccag
     2101 gcgttcgaac gcttcgcggt acttggccgc cgtgcgttca cccacctcgg ccggcatcgc
     2161 cggggccggc gccttcttgt tccacggctg gccgtcgatg cgcaccgact cgagccagtc
     2221 gcgcacgaac tgcttgtcgt agctgggcgg gttgctgccc tcggcgtagc cgtcgaccgg
     2281 ccagtagcgc gaggagtcgg gcgtcagcac ctcgtccatc agcgtgagcg tgccgtcggc
     2341 gtcgaggccg aactcgaact tcgtgtcggc gatgatgatg cccttggtca gcgcgtattc
     2401 ggcggcccgc tcgtacagcg cgatggagac gtcgcgcacg tggttggcca gctcgttgcc
     2461 gatcatggcg ctggccacct
//
LOCUS       DATCRO010000920         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_303100, whole genome shotgun sequence.
ACCESSION   DATCRO010000920 DATCRO010000000
VERSION     DATCRO010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303100"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VIN75_24955"
     CDS             <1..219
                     /locus_tag="VIN75_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8977488.1"
                     /translation="FANQTIAQIELFTKPDAYQAGKVYVLPKHLDEKVARLHLKKVGA
                     MLTELTDVQAAYIGVDKNGPYKPDTYRY"
     gene            251..1036
                     /locus_tag="VIN75_24960"
     CDS             251..1036
                     /locus_tag="VIN75_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY8977489.1"
                     /translation="MRADQLLVERGLAASRSQAVRLIAGGLRWRDAGSGDAWRNVVKN
                     KDDVPESAELELVDAAEARYVSRGGLKLEGALAASGIDPAGWLCLDVGQSTGGFTDCL
                     LQRGAARVVGVDVGHGQLHARLREDERVVAIEGVNARALSADDLGEEGDEGGPRFDLI
                     VGDLSFISLTLVLPAVVGFMADEGRLLMLVKPQFELQPGQIGKGGIVRDDALYAVVEK
                     RLRDACEALGLRVLQWFDSPIAGGDGNREFFIHAVRAVQANGS"
     gene            1047..1871
                     /gene="metF"
                     /locus_tag="VIN75_24965"
     CDS             1047..1871
                     /gene="metF"
                     /locus_tag="VIN75_24965"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY8977490.1"
                     /translation="MRLPLSFEFFPTKTPEGAVKLRAVRQQLYARKPQFCSVTYGAGG
                     STHQGTFGAVQEILSEGVDAASHFSCIGATRATVREQLAELKAMGVKRLVALRGDLPS
                     GYGIGGEFQYASDLVGFIREETGRDFHIEVACYPEIHPQARSPEADLQAFAAKVKAGA
                     DSAITQYFFSPEAYFRFVDDVRKLGLDTPIVPGIMPITSSTQLMRFSDACGAEIPRWI
                     RLRLQSFGDDTASIKAFGLDVVTDLCARLQEGGAPALHFYTMNQSAATLAVLERLD"
     gene            1934..2554
                     /locus_tag="VIN75_24970"
     CDS             1934..2554
                     /locus_tag="VIN75_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8977491.1"
                     /translation="MPPATDAEGGGDGTKLLPLPRAIAVPPGAAARSSVPAVRYDLAV
                     SGMGEQAPDDGIPDDGPREIFERGGASWYGIQFHQRKTASGERFDMSAMTAAHKTLPF
                     NTRVCVRSLVNGSEVLVRINDRGPYSAGRVIDLSRAAADRIGLTGLGIKQVALTVIDR
                     DGMRCGGLPVEAGDAAAPVASSEPQQRARAQAPARRKAVVPPRRRR"
BASE COUNT          389 a          886 c          940 g          385 t
ORIGIN      
        1 ttcgcgaacc agaccatcgc gcagatcgag ctgttcacca agcccgacgc ctaccaggcc
       61 ggcaaggtct acgtgctgcc gaagcacctc gacgagaagg tcgcgcgcct gcacctgaag
      121 aaggtcggtg ccatgctgac cgagctgacc gacgtgcagg ccgcctacat cggcgtcgac
      181 aagaacggcc cctacaagcc ggacacctac cgctactgag cgggggtcgc agcgacagcc
      241 gcagcagcgc atgcgcgccg atcagttgct ggtggagcgc ggcctggccg cgtcgcgttc
      301 gcaggccgtg cggctcatcg ccggcggctt gcgctggcgt gacgcgggct cgggcgacgc
      361 ctggcgcaat gtcgtcaaga acaaggacga cgtgccggag tctgccgagc tcgagctggt
      421 cgatgcggcg gaagcgcgct atgtttcgcg cggcgggctc aagctcgagg gcgcgctggc
      481 ggcgagcggc atcgacccgg ccggctggct gtgcctcgac gtgggccagt cgaccggcgg
      541 cttcaccgac tgcctgctgc agcgcggcgc cgcccgggtg gtgggcgtcg acgtaggcca
      601 cggacagctg catgcgcggc tgcgcgagga cgagcgcgtg gtggccatcg agggcgtcaa
      661 tgcgcgtgcg ctgtcggccg acgatctcgg cgaagaaggc gatgaaggtg ggccacgctt
      721 cgacctgatc gtgggcgacc tgtcgttcat ctcgctcacg ctggtgctgc cggcggtcgt
      781 cggattcatg gccgatgaag ggcgcctgct gatgctggtc aagcctcagt tcgagctgca
      841 gccgggccag atcggcaagg gcggcatcgt gcgcgacgac gcgctgtacg cggtggtcga
      901 gaagcgcctg cgcgatgcct gcgaggcgct cggcctgcgg gtgctgcagt ggttcgacag
      961 cccgatcgcc gggggcgacg gcaaccgcga gtttttcatt cacgccgttc gcgccgttca
     1021 ggcgaacggt tcctaaggag acgcaggtgc gccttccgtt gagtttcgaa ttcttcccga
     1081 ccaagacgcc cgaaggcgcg gtcaagctgc gcgcggtgcg ccagcagctg tatgcgcgca
     1141 agccgcagtt ctgctcggtc acctacggcg cgggcggttc cacgcaccag ggcaccttcg
     1201 gtgcggtgca ggagatcctg tccgaaggcg tggacgccgc gagccacttc tcgtgcatcg
     1261 gcgccacgcg cgccaccgtg cgcgagcagc tcgccgagtt gaaggcgatg ggcgtcaagc
     1321 gcctggtggc gctgcgcggc gacctgccca gcggctacgg catcggcggc gagttccagt
     1381 atgcgagcga cctcgtgggc ttcatccgcg aggagaccgg ccgcgacttc cacatcgagg
     1441 tggcctgcta ccccgagatc cacccgcagg cccgctcgcc cgaggccgat ctgcaggcct
     1501 tcgccgccaa ggtgaaggcg ggcgccgact cggcgatcac gcagtacttc ttcagccccg
     1561 aggcgtattt ccgcttcgtc gacgacgtgc gcaagctggg gctcgacacg ccgatcgtgc
     1621 cgggcatcat gccgatcacg agctcgacgc agctcatgcg cttctcggac gcctgcggcg
     1681 cagagatccc gcgctggatc cgcctgcggc tgcagagctt cggcgacgac acggcgtcga
     1741 tcaaggcctt cggcctcgat gtggtcaccg acctgtgcgc gcgcctgcag gaaggcggcg
     1801 cgccggcgct gcacttctac acgatgaacc agtcggcggc cacgctggct gtgctggagc
     1861 gcctcgattg agagggcgcg cggggttccg ggcggcgctt gtcgcggcga cctgcgttct
     1921 tgcgggatgc gccatgccgc ctgccaccga cgccgaaggc ggcggtgacg ggacgaagct
     1981 gctgcccctg ccccgcgcca tcgcggtacc tccgggcgct gccgcgcgct ccagcgtacc
     2041 cgcggtgcgc tatgacctcg ccgtctccgg catgggcgag caggccccgg acgacggtat
     2101 tcccgacgac gggccgcgcg aaattttcga gcgcggcggc gcatcgtggt acggcatcca
     2161 gttccaccag cgcaagaccg ccagcggcga acgcttcgac atgagcgcca tgacggcggc
     2221 ccacaagacg cttccattca acacgcgcgt gtgcgtgcgc agcctcgtca acggcagcga
     2281 ggtgctggtg cgcatcaacg accgcggccc ctattcggcg ggacgcgtca tcgacctgag
     2341 ccgcgcggcg gccgaccgga tcggcctgac ggggctgggc atcaagcagg tggcgctgac
     2401 ggtgatcgac cgcgacggca tgcgctgcgg cggcctgccg gtcgaggccg gcgatgcggc
     2461 ggcgcccgtc gcttcgagcg aaccgcagca gcgggcgcgc gcgcaggctc cggcgcggcg
     2521 caaggccgtg gtgccgcccc gccgccgcag gtaggcaggc agatgcgaca agggcggctt
     2581 gctcagccgt ccgaatcgag
//
LOCUS       DATCRO010000921         8503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_33080, whole genome shotgun sequence.
ACCESSION   DATCRO010000921 DATCRO010000000
VERSION     DATCRO010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33080"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            528..1610
                     /locus_tag="VIN75_24975"
     CDS             528..1610
                     /locus_tag="VIN75_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8977492.1"
                     /translation="MHKISIRPQWTISHDGGDTLGPRLIALLVCVAETGSLAAACQSA
                     GLSYRHAWDTIRDGEAWFGAPLLQMERGRGSRLTPLGEKLVWADRRIAARLAPALDTL
                     ASELEVELQRVIAPPQRLLRIHASHGFAIEKLMAMLVADGVDVERVYTDSLGAAASLH
                     DGACDLACFHVPHGPLEARGWEFHARWLDPREHVVIDITDRTQGLMVAPGNPLDIAGL
                     GDLARPGLRFINRQRGSGTRFLLDALLAQAGVDPAVIVGYEHGEFTHAAVAAFIGSGM
                     ADAGFGLERPARHFKLDFIPLATERYFAVCRCDALARPEIEQARAILRSDAFRSEVDA
                     LPGYRARNSGEVTPLGDILAAAGVDV"
     gene            complement(1722..2066)
                     /locus_tag="VIN75_24980"
     CDS             complement(1722..2066)
                     /locus_tag="VIN75_24980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977493.1"
                     /translation="MNVVKSIVSASIVVAGALLAGASAQAACPSVSNVQARIVEHANG
                     DDMGGLRSFVWKTSIVYRINMVDVRNNLDKWRMAVDCRAAAAAGQQAVPVAGQAPAAD
                     TTDAAGVHVAAR"
     gene            complement(2402..2947)
                     /locus_tag="VIN75_24985"
     CDS             complement(2402..2947)
                     /locus_tag="VIN75_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977494.1"
                     /translation="MTTMNRYAPPKAAVEDRPAEAEMWREGKVLVMRKGGAFPDRCIK
                     CNAPSVEPRRRYRLTWHSPWLYLLILLALLLYALVAFIVRKTAIVHVGLCERHQKRVL
                     WGRIIGWGGLALELALLCAAAMLHEELFGIAAGLLVVPWLIASVVVNRLLLPQRIDDK
                     YVRLKGCGPDFLRSLPDRGGY"
     gene            complement(3024..4310)
                     /locus_tag="VIN75_24990"
     CDS             complement(3024..4310)
                     /locus_tag="VIN75_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8977495.1"
                     /translation="MRLRLTWPRVILALALLIALAAALVAWLNLRGEDPLTGSADSFV
                     ATPEQVARGAYLARAGNCAACHTDRGGAAFAGGKGIATPFGTVYASNLTPDAKTGIGT
                     WSAAQFWRALHNGRSADGHLLYPAFPYPNFTEITRPDADALLAFLRSQVPVAQANRAH
                     DLRFPYNLQASLAVWRALFFTPGVYEPQQGRSPQWNRGAYLVRALGHCAACHSPRNAF
                     GATRDSLELSGGLIPLQNWYAPSLASSAEAGVADWPDADVAALLKTGFTPRGAALGPM
                     AEVVFRSTQYLDDADVAAMTVFLKSLPQGPAHAVVAPAIGQQALAHGAALYKDRCASC
                     HGADGQGGSTPQGVVYVPLAGNRKVLLESPANLIHIVVNGGFPPTTAGNPRPYGMPPF
                     GPSMKDEDIAMLATYVRTAWGNAAPAVSALDVLEAR"
     gene            complement(4307..4963)
                     /locus_tag="VIN75_24995"
     CDS             complement(4307..4963)
                     /locus_tag="VIN75_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8977496.1"
                     /translation="MAAATAGPPQGVPDTLAQRMQPCSACHGREGRATQEGFFPRIAG
                     KPAGYLYNQLDNFRSGRRAYPTMTYFVEQMSDAYLHEIADYFASLDLPYAPPQTAGAP
                     ADEIARGEALVRQGDAAHGIPACAQCHGATMTGVLPAIPGLLGLPRGYLVEQFGAWRT
                     GQRKALAPDCMARVATMLTPADITAVATWLSSQPVAVGPAPAEAIARPLPLACGSVPQ
                     "
     gene            complement(5248..6549)
                     /gene="glcF"
                     /locus_tag="VIN75_25000"
     CDS             complement(5248..6549)
                     /gene="glcF"
                     /locus_tag="VIN75_25000"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY8977497.1"
                     /translation="MQTNLSPEFAGTPDGQEAEGILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKQVLEGAEPTRATQLHLDRCLTCRNCESTCPSGVEYGKLVDIGRRVV
                     DAKVQRPAGERFTRWLLREGITSPLFAPAMKLGQAVRPLLPQSLKAKVPVRPDAATAA
                     RHGLWPQRTHDRKVLMLAGCVQPAMKPNINAATARVLDLAGVQAVTPDGAGCCGAVRS
                     HGGDHDGGLDDMRRNIDAWWPWVAGLTDAGAVEAIVMNASGCGVAVKDYGHALAHDPD
                     YAEKAARVSALTRDVSEVIDGLMPALLSHWKGVRERRGAESPEHAPGVPLVFHPPCTL
                     QHGQQLRGGVEQILAAFDFDVRLAASESHLCCGSAGTYSVLQPAIATQLRDRKLAALA
                     ASQPDPRAIVSANIGCISHLQSGTATPVRHWIELVDERLAG"
     gene            complement(6556..7671)
                     /gene="glcE"
                     /locus_tag="VIN75_25005"
     CDS             complement(6556..7671)
                     /gene="glcE"
                     /locus_tag="VIN75_25005"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY8977498.1"
                     /translation="MNDTLIGLAERVRQAAAAKAILRIRGGSTKQAMAGVISDAATLD
                     MRAYSGIVDYAPSELVITARAGTPLVELERVLAERGQCLPFDPPRFADADGRLAGTVG
                     GMVASGLAGPGRAAVGGVRDYVLGVLLLNGRGDLMHFGGQVMKNVAGYDVARLTAGSW
                     GALGAICEVSLKVLPRPVATTTLRFDMRQAEALENLHAWAGQPLPLNASVWWRETLAI
                     RLRGANAAVASAVKRLGGEVIPQEIGDAFWEGLRDQRDEFFIEAREAAARENSALWRL
                     SLPPTTPVLPSFLDTLIEWGGAQRWIVAPAEDTLVMHRAERAGGSAFRVSGGVGDGAG
                     YATPLAPALLAIHRRLRDSFDPHRVFVPGRPMRFQDS"
     gene            complement(7680..>8503)
                     /locus_tag="VIN75_25010"
     CDS             complement(7680..>8503)
                     /locus_tag="VIN75_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8977499.1"
                     /translation="LTPRPQVARCIMASFSDVRHAGDAVAAIIADGLIPAGLEMMDKP
                     MTAAVEAFVHAGYDLDAAAILLCESDGTPEEVEEEILRMEAVLVRAGATRLEISANEA
                     QRLKFWSGRKNAFPASGRLSPDYMCMDSTIPRARLADMLLAIADMERKFGLRCCNVFH
                     AGDGNLHPLILFDANQPDELHRAEAFGAEILELSVAMGGTISGEHGVGAEKLNSMCVQ
                     FSETERAQMLALKAAFDPQGLLNPGKVIPTLHRCAEYGRMHVKRGALAFPELERF"
BASE COUNT         1162 a         3055 c         3091 g         1195 t
ORIGIN      
        1 tgacggttga catcttgttc cttttgatga cctgcgggca tcgatcgtcg ctgtcccaac
       61 atcgacgact gcccgactgt aagtatcccg tggggatcgt tgcaatatgc ggtcaagctc
      121 taaagacgaa ccctaggatc gacctcgcat gaggcctcct tcgtcaatcc cgatgtcctg
      181 attgagcgtt cagatgctgc agtgccacat ccatcgcgat accgcccgcg gtaccctgcc
      241 tggtacgttt tgttgcaatg caggcggcct gctcgacggt ttaatgcgac atccctgccg
      301 ggcttggggg gattaccccc ctgggcatcg gtccgcccgc gttgggcggg tcgacgcgcc
      361 gggcgaaggg aaccggccgg gacgcgtcgc cgagatccca gccggcgatg ccgaatccgc
      421 atcgatgtcg acagcgtgat agcgcggccg ccgtgttcgc ggcaccgccg gcgcccgggt
      481 cgcattccgg agaaactcac gcttgcggcg gagcgccgcg ccacctcatg cacaagatct
      541 ccatccgtcc ccagtggacg atctcccacg acggcggcga cacgctgggt ccgcgcctga
      601 tcgccctgct ggtctgcgtc gccgagacgg gcagcctcgc cgccgcctgc cagagcgcgg
      661 gcctgtccta ccgccacgcc tgggacacga tccgcgacgg cgaagcctgg ttcggcgcgc
      721 cgctgctgca gatggagcgt ggccgcggat cgcgcctgac gccgctgggc gagaagctgg
      781 tgtgggccga ccgccgcatc gccgcgcgac tcgcgccggc cctggacacg ctggcctccg
      841 agctggaggt ggagctgcag cgcgtcatcg cgccgccgca gcgcctgctg cgcatccacg
      901 cgagccatgg cttcgccatc gagaagctga tggcgatgct ggtggccgac ggcgtcgacg
      961 tcgagcgcgt ctataccgac agcctcggcg ccgccgcctc gctgcacgac ggcgcctgcg
     1021 acctcgcctg cttccacgtg ccgcacggcc cgctcgaggc gcgcggctgg gagttccatg
     1081 cgcgctggct ggatccgcgc gagcacgtcg tcatcgacat caccgaccgc acgcaaggcc
     1141 tgatggtcgc cccgggcaac ccgctggaca tcgcgggact gggcgacctt gcgcggccgg
     1201 gcttgcgctt catcaaccgc cagcgcggct cgggcacgcg cttcctgctg gacgcgctgc
     1261 tcgcgcaggc gggggtcgac ccggccgtca tcgtcggcta cgagcacggc gagttcacgc
     1321 atgcggccgt agccgccttc atcggcagcg gcatggccga cgccggcttc ggcctcgagc
     1381 gcccggcgcg ccatttcaag ctggacttca tcccgctggc caccgaacgc tacttcgcgg
     1441 tctgccggtg cgacgcgctg gcgcggcccg agatcgagca ggcgcgcgcg atcctgcgca
     1501 gcgacgcgtt ccgttcggag gtggacgcgc tgcccggcta ccgcgcccgc aacagcggcg
     1561 aggtgacgcc gctgggcgac atccttgcgg cggcgggcgt ggacgtctga cgctcccgcg
     1621 tcccgggcgc gtcggccatc accgccgccg agatcgacgc ccacggcggc acgaccggtg
     1681 cgccgggcgg ctcgcgcacc atcgcgtctg aggcgcgcgc ctcaacgcgc ggcgacgtgc
     1741 acgcccgccg cgtcggtcgt gtcggccgca ggcgcctgcc ccgcgaccgg aacggcctgc
     1801 tgcccggccg cggccgcggc gcggcaatcg accgccatgc gccacttgtc caggttgttg
     1861 cgcacgtcga ccatgttgat gcggtagacg atggacgtct tccacacgaa ggaacgcagc
     1921 ccgcccatgt cgtcgccgtt ggcgtgctcg acgatgcggg cctggacgtt cgagacggag
     1981 gggcaggccg cctgggcgga tgcgccggcc agcaatgcgc cggcgacgac gatggaagcg
     2041 gagacgatgc tcttgacgac gttcatgacg aactcctcgg atcggattgg agggagaacc
     2101 aggaagtcgg tcgccaagtc gctggtggcg acggccgtgg cgatcggctg ggcgcgcgcg
     2161 tggccgcgca tcggcgcggg cgcgccatgc gggaaggacc accggcggcc ggtcgccggg
     2221 tcgctgttgg cgacgggcgg ggcgatcggt cgagggtgag aatggccctg cccgcatcgg
     2281 cgcgcgagcg cgatgcgacg aatgccgtca aacggcggct gaagtacgcc ggggcgcgac
     2341 aaacaccgga tggaccggat ccgacgccct tcgcgccgtc gcgcgcggcg ccgccgccgg
     2401 ctcagtagcc gccgcgatcg ggcagcgagc gcaggaagtc cggcccgcag cccttcaggc
     2461 gcacgtactt gtcgtcgatg cgctggggca acagcaggcg gttgaccacc acgctggcga
     2521 tcagccacgg caccaccagg agtccggctg caatgccgaa gagctcttca tgcagcatcg
     2581 ccgccgcgca caacagcgcc agctcgaggg ccaggccgcc ccagccgatg atgcgccccc
     2641 acagcacgcg cttctggtgt cgctcgcaca gccccacgtg cacgatcgcc gtcttgcgca
     2701 cgatgaacgc cacgagggcg tacagcagca gcgccagcag gatcagcagg tagagccagg
     2761 gcgagtgcca ggtcaggcgg tagcgccgcc gcggttccac gctcggcgcg ttgcacttga
     2821 tgcagcggtc gggaaacgcc ccgcccttgc gcatcacgag caccttgccc tcgcgccaca
     2881 tctcggcctc ggccggccgg tcctccactg cggccttcgg cggcgcgtag cgattcatcg
     2941 tcgtcatggg ttccctcccc gttgtgccgc gcgccgatcc ggtcgtggcc ggcgctccgc
     3001 ggcgattcgt cccgcgcgcc tcctcagcgc gcctccagca cgtccagcgc actcaccgcc
     3061 ggcgccgcgt tgccccaggc cgtgcgcacg taggtggcca gcatggcgat gtcctcgtcc
     3121 ttcatcgacg gcccgaacgg cggcatgccg tagggcctcg ggttgcctgc cgtcgtgggc
     3181 gggaagccgc cgttgacgac gatgtggatc aggttggcgg gcgactccag cagcaccttg
     3241 cggttgccgg ccagcggcac gtagacgacg ccctgcgggg tgctgccgcc ctgcccgtcg
     3301 gcgccgtggc acgacgcgca gcggtccttg tagagcgcgg cgccgtgcgc gagcgcctgc
     3361 tggccgatcg ccggcgcgac gacggcgtgc gcgggcccct gcggcagcga cttcaggaac
     3421 accgtcatcg cggcgacgtc ggcgtcgtcc aggtattgcg tgctgcggaa gacgacctcg
     3481 gccatcgggc ccagcgccgc gccgcgcggc gtgaagcccg tcttgagcag cgccgccacg
     3541 tcggcatccg gccagtcggc cacgcccgcc tccgcgctcg aggcgagcga cggcgcgtac
     3601 cagttctgca gcgggatcag gccgccgctg agctccaggc tgtcgcgcgt cgcgccgaaa
     3661 gcgttgcgcg gcgagtggca ggccgcgcag tggcccagcg cccgcaccag gtaggcgccg
     3721 cggttccact gcggcgagcg cccctgctgc ggctcgtaga cgcccggcgt gaagaacagc
     3781 gcgcgccaca ccgccagcga ggcctgaagg ttgtacggga agcgcaggtc gtgcgcgcga
     3841 ttggcctgcg ccacgggcac ctgcgagcgc aggaacgcga gcagcgcgtc ggcgtcgggg
     3901 cgcgtgatct cggtgaagtt cgggtacggg aacgccggat acagcaggtg cccgtcggcc
     3961 gaacgcccgt tgtgcagcgc gcgccagaac tgcgcggccg accaggtacc gatgccggtc
     4021 ttcgcgtccg gcgtgaggtt gctcgcgtag accgtgccga acggcgtggc gatgcccttg
     4081 ccgcccgcga acgccgcgcc gccgcggtcg gtgtggcaag ccgcgcagtt gccggcgcgc
     4141 gcgaggtagg cgccgcgggc gacctgttcg ggcgtggcga cgaaggagtc ggcgctgccc
     4201 gtgagcgggt cctcgccgcg caggttgagc caggcgacca gcgccgcggc cagggcgatc
     4261 aacagcgcca gcgccaggat cacgcgcggc caggtcaggc gcagcctcat tgcggcacgc
     4321 tcccgcaggc gagcggcagc ggccgggcga tcgcctcggc gggcgccggc cccacggcca
     4381 ccggctgcga cgacagccat gtcgccacgg cggtgatgtc ggccggcgtc agcatcgtgg
     4441 cgacgcgggc catgcagtcg ggcgcgagcg ccttgcgctg gcccgtgcgc cacgcgccga
     4501 actgctccac gagatagccg cgcggcaggc ccagcagacc ggggatggcg ggcagcacgc
     4561 cggtcatcgt cgcgccgtgg cactgcgcgc aggccgggat gccgtgcgcc gcgtcgccct
     4621 ggcgcaccag cgcctcgccg cgcgcgatct cgtcggccgg cgcgccggcc gtctgcggcg
     4681 gcgcgtaggg caggtccagc gacgcgaagt agtcggcgat ctcgtgcagg tacgcgtcgg
     4741 acatctgctc gacgaagtac gtcatcgtcg gataggcgcg ccgtccgctg cggaagttgt
     4801 ccagctggtt gtacaggtag ccggcgggct tgcccgcgat gcgcgggaag aagccttcct
     4861 gcgtggcccg cccttccctg ccgtggcagg cgctgcaggg ctgcatgcgc tgcgcgaggg
     4921 tgtcgggcac cccctgcggc gggccggccg tcgccgcggc catcgtcgtc gacgatccga
     4981 tcaagacggc catgcggcga agcagcgact tgacggaaag ggagttcatg ccgcgactgt
     5041 atctcgcgac gccgtcaagg ggccgtcatt gcgcgcgcag tcgcggaatc cacggcggaa
     5101 cgagaagatt cgcacgcgcc agcgtggatc ctgcgactgc gcgcaggatg acggcccgtc
     5161 ggtgcgaccg ctttctcagg acgacggcgg tctctatggc atccccgacg agcgggggca
     5221 ttcgtccctc gggcggccgg gctggactca tccggccaac cgctcgtcga cgagttcgat
     5281 ccagtggcgc accggtgtcg cggtgcccga ctgcaggtgc gagatgcagc cgatgttggc
     5341 cgacacgatg gcgcgcggat ccggctgcga cgcggccagc gccgcgagct tgcggtcgcg
     5401 caactgcgtg gcgatggccg gttgcagcac cgagtacgtg ccggcggaac cgcagcaaag
     5461 gtgcgattcg gacgcggcca ggcgcacgtc gaagtcgaac gccgcgagga tctgttcgac
     5521 gcccccgcgc aactgctgcc cgtgctgcag cgtgcacggc ggatggaaca ccagcggcac
     5581 gcccggcgcg tgctcgggcg actccgcgcc gcggcgctcg cgcacgccct tccagtgcga
     5641 cagcagcgcc ggcatcaggc cgtcgatcac ctcgctcacg tcgcgcgtca gggcgctgac
     5701 gcgggcggcc ttctcggcgt agtcgggatc gtgcgccagc gcgtggccgt agtccttgac
     5761 ggcgacgccg cagcccgacg cgttcatcac gatggcctcg accgcgccgg cgtcggtcag
     5821 gccggccacc cacggccacc aggcgtcgat gttgcggcgc atgtcgtcca gcccgccgtc
     5881 gtggtcgccg ccgtggctgc gcaccgcgcc gcagcagccg gcgccgtcgg gcgtgacggc
     5941 ctgcacgccg gccaggtcga gcacgcgcgc ggtggccgcg ttgatgttgg gcttcatcgc
     6001 gggctgcacg cagccggcca gcatgagcac cttgcggtcg tgcgtgcgct gcggccacag
     6061 gccgtgccgc gcggcggtgg ccgcatcggg ccggaccggc accttggcct tgagcgactg
     6121 cggaagcagc gggcgcaccg cctggcccag cttcatcgcg ggcgcgaaca gcggcgacgt
     6181 gatgccctcg cgcagcagcc agcgcgtgaa gcgttcgccg gccggccgct gcaccttggc
     6241 gtcgacgacg cggcggccga tgtccaccag cttgccgtac tcgacgcccg aggggcacgt
     6301 gctctcgcag ttgcggcagg tcaggcagcg gtccaggtgc aactgcgtgg cgcgcgtcgg
     6361 ctcggcgccc tcgagcacct gcttcatcag gtagatgcgg ccgcgcgggc cgtcgagctc
     6421 gtcgcccagc agctggtacg tcgggcaggt ggccgtgcag aagccgcagt gcacgcactt
     6481 gcgcaggatg ccctcggcct cctggccgtc gggcgtgccg gcgaattccg gggaaaggtt
     6541 ggtctgcatg gaagctcagg aatcctggaa gcgcatcggg cgccccggga cgaacacgcg
     6601 gtggggatcg aacgaatcgc gcaggcggcg gtggatggcc agcagcgcgg gcgccagcgg
     6661 cgtggcgtag cccgcgccgt cgcccacgcc gccgctgacg cggaatgcgc tgccgccggc
     6721 gcgctcggcg cggtgcatga cgagcgtgtc ctcggccggc gcgacgatcc agcgctgcgc
     6781 gccgccccac tcgatcagcg tgtcgaggaa ggacggcagc accggcgtgg tgggcggcag
     6841 cgacagccgc cacagcgcgc tgttctcccg cgccgcggcc tcgcgggcct cgatgaagaa
     6901 ctcgtcgcgc tggtcgcgca gcccttccca gaacgcgtcg ccgatctctt gcgggatgac
     6961 ctcgccgccc agccgcttga ccgccgacgc gaccgccgcg ttggcgccgc gcaggcggat
     7021 cgcgagcgtc tcgcgccacc acacgctggc gttgagcggc agcggctggc cggcccaggc
     7081 gtgcaggttc tccagcgcct cggcctggcg catgtcgaag cgcagcgtgg tcgtggccac
     7141 cggcctcggg agcaccttca gcgagacctc gcagatcgcg ccgagcgcgc cccacgagcc
     7201 cgcggtcagg cgcgcgacgt cgtagccggc gacgttcttc atcacctggc cgccgaagtg
     7261 catcaggtcg ccgcggccgt tgagcagcag cacgcccagg acgtagtcgc gcacgccgcc
     7321 gacggccgcg cgcccgggcc cggccaggcc ggaggcgacc atgccgccga cggtgcccgc
     7381 gagtctgccg tcggcatcgg cgaagcgcgg cggatcgaac ggcaggcact ggccgcgctc
     7441 ggccagcacg cgctcgagtt cgaccagcgg cgtgccggcg cgcgccgtga tgacgagctc
     7501 gctgggcgcg tagtcgacga tgcccgaata ggcgcgcatg tccagcgtcg cggcgtcgga
     7561 gatgacgccg gccatggcct gcttggtgct gccgccgcgg atccgcagga tggccttggc
     7621 ggcggcggcc tggcgcacgc gttcggccag gccgatcagg gtgtcgttca ttctttccgt
     7681 cagaaacgtt ccagctcggg gaaggcgagc gcgccgcgct tgacgtgcat gcgcccgtat
     7741 tcggcgcagc ggtgcagcgt cgggatgacc ttgcccgggt tcagcagccc ctgcgggtcg
     7801 aaggccgcct tgagcgccag catctgcgcg cgctcggtct cggagaactg cacgcacatc
     7861 gagttgagct tctcggcgcc cacgccgtgc tcgccgctga tggtgccgcc catggccacc
     7921 gacagctcca ggatctcggc gccgaaggcc tcggcccggt ggagttcgtc gggctggttg
     7981 gcgtcgaaca ggatcagcgg gtgcaggttg ccgtcgccgg cgtggaacac gttgcagcag
     8041 cgcaggccga acttcctttc catgtcggcg atggccagca gcatgtcggc cagccgcgcg
     8101 cgcgggatcg tcgagtccat gcacatgtag tcgggactca gccggcccga cgcggggaac
     8161 gcgttcttgc ggccgctcca gaacttcagg cgctgcgcct cgttggcgct gatctccagc
     8221 cgcgtggcgc cggcgcgcac cagcacggcc tccatgcgca ggatctcctc ctcgacttcc
     8281 tcgggcgtgc cgtcgctctc gcacagcagg atcgcggcgg cgtcgaggtc gtagcccgcg
     8341 tgcacgaagg cctcgacggc ggccgtcatc ggcttgtcca tcatctcgag cccggcgggg
     8401 atcaggccgt ccgcgatgat ggccgccacc gcgtcgccgg cgtggcgcac gtccgagaag
     8461 ctggccatga tgcagcgcgc cacctgcggc ctgggcgtga gct
//
LOCUS       DATCRO010000922         8109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1011707, whole genome shotgun sequence.
ACCESSION   DATCRO010000922 DATCRO010000000
VERSION     DATCRO010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011707"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VIN75_25015"
     CDS             <1..562
                     /locus_tag="VIN75_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8977500.1"
                     /translation="IWMPDVDGITLLKEWGATAQLNMPVIMMSGHGTIDTAVEATKYG
                     AIAFLEKPITLQKLLRAVEQGLARPSSRPAAAADVQAAMLQGNGGYRPDAMTVEAAMT
                     GGLGLQPAAPLNLGPQPEQSFDLDRPLREARDAFEKSYFEFHLARENGSMTRVAEKTG
                     LERTHLYRKLKQLGVDLSRGKRGAGG"
     gene            667..742
                     /locus_tag="VIN75_25020"
     tRNA            667..742
                     /locus_tag="VIN75_25020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:700..702,aa:Phe,seq:gaa)
     gene            833..1078
                     /locus_tag="VIN75_25025"
     CDS             833..1078
                     /locus_tag="VIN75_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977501.1"
                     /translation="MKALVRWVSLIAFVLAAVWVYKEPKIEAWIIAATTLGAFLATFL
                     APESSASGQSQRVTGGSQAFQAGRDIKNSGNRAGDDA"
     gene            1071..2138
                     /locus_tag="VIN75_25030"
     CDS             1071..2138
                     /locus_tag="VIN75_25030"
                     /inference="COORDINATES: protein motif:HMM:NF045477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPO_1073/Vpar_1526 family protein"
                     /protein_id="HEY8977502.1"
                     /translation="MPERQSQSVAESSTAYQAGRDVVLIQGVSASEARQIAHDLFRAN
                     LMEFKAEAAEVARQRCEEVTTAFLEKLQTENPEGLNQAREPDFQDALFSVQKEHAKAG
                     DKELSDLLVDLLVDRTKERSRTILQLVLNEALRTAPKLTSAQTATLAVVFFFRNVRRM
                     NGIPHIAELGAHFQRSMEQAAAGFSNSASTFNHLAFTGCTTDSLAASSLPDVLSRTYP
                     GLFTIGFEQAALEQAQLSDAGKAMLIIPCMDDPTRFQVNALNDEDFDAKCAAVGLSQE
                     EKVRATALFKSYSMPHDKVQSKMVEAAPFMSDIFAAWSAADAKHHALGRYQLTSVGMA
                     IGHASIKRYIGEFAPLDIWIN"
     gene            2167..2544
                     /locus_tag="VIN75_25035"
     CDS             2167..2544
                     /locus_tag="VIN75_25035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977503.1"
                     /translation="MNTNQAEDVGALARANGRARKDKCMLLGFELHSWETAMIVSLIV
                     AALAAAAVGITTWGALKLTRAENAERAVQIAEANKRAEEARLALAQFREPRSIDSRMI
                     VVYKSPRLGNSRGDGSTCSARGL"
     gene            3015..3293
                     /locus_tag="VIN75_25040"
     CDS             3015..3293
                     /locus_tag="VIN75_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977504.1"
                     /translation="MDQLLTPNQEILRNRIRFALRRATGFVFDIDAMLRKPELRAKRI
                     GLWRDVADHELSSLLDQLETEFQADAADEEEAHRTVVIHRPEAGATHR"
     gene            complement(3441..3971)
                     /locus_tag="VIN75_25045"
     CDS             complement(3441..3971)
                     /locus_tag="VIN75_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977505.1"
                     /translation="MAATLQAFVSALDDDPYCGTKPPHWPFGPLRAGTIQEIIATREA
                     EVAINPQPLPPIARDTAAAAFQQVRLFQLGRSLQARSGAVAEMGGQISGFASEMFDGT
                     CGSVPLSVLIAWLLHHNPPPPQPWWLEEMSQVVSQLNLAARMEGNVGGAIQHAALTTL
                     KEQLGALRGQKAQQAR"
     gene            complement(4195..6885)
                     /locus_tag="VIN75_25050"
     CDS             complement(4195..6885)
                     /locus_tag="VIN75_25050"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY8977506.1"
                     /translation="MPLFRLKPCPLPVASLALMALLTACGGGSPTGSTTPVPPAATYT
                     AASGVAQKGPMAAGSTVTAQELGLNLSATGNHYGYTTDATGAFTPSDKYASQYLSLTA
                     SGSYADEVTGQSSDGAVTLQSYADLKAEQVVNVNVLTTLAFTRTNTLLNTNGMTFAAA
                     RAQAETEVLAAFGIVLPASPGAFGTLDAGAGTDGGSLLAALSSVVVQGRASADVGALL
                     ASLQADINAHGAAISAASQQALAVSEQALNLNAVAAHLAATYGRSFDANQLAQWIDQD
                     GDGVVARDEFRVDGAAAGASFTLPADFVTAHAGAAISASAGQLTVNGTAASGAVTLAA
                     GDTVALAAPGTLADGVLKIYLQAGGARIGRVTFVKGLTSIAITPTTGTLPLGISQRFT
                     ATGTYADGHSADISGAVTWTSSAPAIADIGAATGLADALTLGDTTITATSGAVSGTLP
                     LTTIAATVKSIVIAPDSLLTGIGITRHFTATGTFSDGSTGDVTASATWSTQTPGIATV
                     SNGAAIGLAAGATSVTARIGNVTAGAVIAVSTDTWTAAPRMPTERVSGHTATLLADGR
                     LLLAGGVKSSGGTAAADLFDPATSTWTPAAPMGVMRSSHTATLLPDGRVLVTGGSTVS
                     TSASQGYVNNASAEIYDPVANTWTATPPMSVARSHHSATLLADGTVLVVGGENAAYLV
                     EASAEIYDPAANTWTAPRAMPLAARSQHTATLLADGKVLVAGGFDIVAGVLTPLASAE
                     LYDPVLHSTNTVVNGVSTTVVTGQDFTAATPLAFARNGHSATRLGDGRVVVIGGGSTQ
                     TEIYDPVAATWTTQSPTAATHTSHGAVLLHDGRILVVGGTQFAQPGAELFDPSTGQWT
                     AASQMLVARGNPTATLMPDGSVLACGGALDSAGIDCEEWW"
     gene            7160..7978
                     /locus_tag="VIN75_25055"
     CDS             7160..7978
                     /locus_tag="VIN75_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8977507.1"
                     /translation="MADESPAPADESPDAAGPRPGDAFVQSFARGLAVLRSFGADAPA
                     QTLTSAAERSGLTRAGARRILLTLQQLGYVETEGRLFRLTPRVMELGFAYLSSQPVWH
                     LAQPVMEELVQDIHESSSAAVLDGDDIVYVLRVPAKKIMAISLGPGSRLPAYCTAMGR
                     VLLAGLPETERRARLERAPRPARTARTVTDVDALLAVLDGVRREGHALVHGELEEGLV
                     SMAAPIVNRNGRVVAAINVSAQDQRMPPDQMRKRLLPRLLESAVSINTLMRMQD"
BASE COUNT         1255 a         2686 c         2907 g         1261 t
ORIGIN      
        1 catctggatg cccgacgtcg acggcatcac gctgctcaag gaatggggcg ccacggcgca
       61 actgaacatg ccggtcatca tgatgagcgg ccacggcacc atcgacaccg ccgtcgaggc
      121 caccaagtac ggcgccatcg cgttcctcga gaagccgatc accctgcaga agctgctgcg
      181 cgccgtcgag cagggccttg cccgcccgtc ctcgcgtccg gctgccgccg ccgacgtcca
      241 ggccgccatg ctccaaggca acggcggtta ccgccccgac gcgatgaccg tcgaagccgc
      301 gatgaccggc ggcctcggcc tgcagcccgc cgccccgctg aacctgggcc cacagcccga
      361 acagagcttc gacctcgacc gccccctgcg cgaggcccgc gacgccttcg agaagagcta
      421 cttcgagttc cacctcgccc gcgagaacgg ctcgatgacc cgcgtcgccg agaagaccgg
      481 cctggagcgc acccacctgt accgcaagct caagcaactg ggcgtcgatc tgtcgcgcgg
      541 caagcgcggc gcgggcggct gagcctcctc tccagaaaat ttccccggcc ggcggttcgg
      601 cacccccaaa ctctgctaga ctagcgggct tcgctgatcg ctcggcgaag caagtctgaa
      661 gaagacggcc tggtagctca gttggtagag cagcggattg aaaatccgcg tgtcggtggt
      721 tcgattccgc cccaggccac cacccaattt cataggaagc cccgtacagt tcagctgtcc
      781 ggggcttttc tacaattccg gcctgttttc tacaaaccaa ggggattggt caatgaaggc
      841 attggttcgg tgggtgagct tgatcgcatt tgtcctggca gcggtttggg tctacaagga
      901 gccaaagatt gaagcctgga tcattgctgc gacgactttg ggcgctttcc ttgccacgtt
      961 tctggcgcca gaaagcagtg cgagcggaca gtctcaacgc gtcaccggag gttcccaggc
     1021 tttccaggcc gggcgagaca tcaagaactc cgggaatcga gcaggcgacg atgcctgaga
     1081 ggcagagtca atcggttgcc gaatcgagta ctgcatacca agctggccgc gacgtcgtac
     1141 tgatacaggg ggtctcggct tctgaggctc ggcagatcgc gcatgacttg tttcgcgcaa
     1201 atctgatgga gttcaaggcg gaagcagccg aagtggcccg gcagcggtgc gaggaggtta
     1261 cgacggcatt ccttgagaag ctacagacgg aaaatcctga ggggctcaat caggcccgcg
     1321 agcctgattt tcaagatgcc ttgttctccg ttcaaaagga gcacgcaaag gcaggcgaca
     1381 aagagctcag cgatctacta gtcgatcttc tagtcgaccg tacgaaggag cgatcgagga
     1441 cgattcttca acttgtattg aacgaggcgc ttcggacagc acccaagctc accagcgcgc
     1501 agacggcgac ccttgcggtt gtcttcttct ttcgtaacgt gagacgcatg aatggcatcc
     1561 ctcacattgc ggaactaggt gcacatttcc agcgttcgat ggagcaagcc gcagctggct
     1621 tctcgaatag tgcgtccacg ttcaatcatc tcgcgttcac gggctgcact accgattcgc
     1681 ttgcggcatc ttcgctgcct gacgttctgt cgcgcacata ccctggtctg ttcaccattg
     1741 gcttcgagca agccgcactc gaacaggcgc agctttcgga cgcaggtaag gcgatgctga
     1801 tcattccctg catggacgat ccgacacgct tccaagtaaa cgcgcttaac gatgaggact
     1861 ttgacgcgaa atgcgccgcg gtcgggttgt cgcaggagga gaaagtacga gcaaccgcgc
     1921 tcttcaagtc ctattccatg ccccatgaca aggtgcaatc caaaatggtg gaggccgcgc
     1981 cttttatgag cgacatcttc gctgcatgga gcgccgccga tgcgaaacat cacgctctgg
     2041 ggcgctatca gctcacgagc gtgggcatgg cgataggcca tgcgagcata aagcgctaca
     2101 tcggtgaatt tgcgccactg gatatctgga ttaactagct atcgaagtgc ctgagcgcgt
     2161 gaaagcatga ataccaacca ggccgaggac gttggagcgc tggctcgagc taatgggcgc
     2221 gccaggaagg acaaatgcat gttgctaggt ttcgagctgc actcgtggga aaccgcaatg
     2281 atcgtttcgt tgattgtggc ggccctggcc gccgccgcgg tcggaatcac gacgtggggc
     2341 gcgctcaagc tcacgcgggc tgaaaacgcc gagagagctg ttcagatcgc ggaggcgaac
     2401 aagcgggctg aagaggcgcg gttggcgctt gcgcagttca gagagccgcg ctccatcgat
     2461 tcgcgtatga tcgtcgtcta caaatcgccc cgactcggaa actcccgcgg agacggctcc
     2521 acatgctcgg cgcgcggttt gtagatcgga tgccgcccaa gtggttgatc cacaaggaaa
     2581 tctgcgggca ttgaaaatcc gcgtgtcggt ggttcgattc cgccccaggc caccagaaca
     2641 tgcgaggccc ctgaatccgg acggagtcag gggctttttg cttcttgtcg ggtctcgatt
     2701 cgacgcgtgt tcttcggccg ttttccggtg caagaaggga cgggtcaatg ggcctgccgg
     2761 caccgatgga aagcggcttc gggagggccc ccgacgctac ggaaaatcga acttgggatt
     2821 gagtgcgaaa gctgcgcact ttcgcacgct cgaatgaccg ttggagaggc gaccaccccc
     2881 atcttgggga gttctgtccc accccaaaac gccgtggcga gcctagactg gcatcaacaa
     2941 atcgcgcaac ggcgaaggac gcgttcctca ggggtgaggg gcgaagcggc cggcaagcgc
     3001 gggagggaac gacgatggac cagcttctga cgcccaacca ggaaatcctg cgcaaccgca
     3061 tccggttcgc gctgcgccgc gcgaccggct tcgtgttcga catcgacgcg atgctgcgca
     3121 agcccgagct gcgcgccaag cgcatcggcc tgtggcgcga cgtcgccgat cacgaactga
     3181 gcagcctgct cgaccagctc gagacggaat tccaggccga cgcggcggac gaggaagagg
     3241 cgcaccgcac cgtggtcatc caccgccccg aggccggcgc gacgcaccgc tgaggcgcgg
     3301 cccgcggcga gcggcggcgg cggtccgcgg gacgcggtcg tccacggcgc ggatgcgccg
     3361 atcgacatgg acgacgccgg gcatgcccgg cgtcgtcgtt tcgggcgggc gcgcgctcgc
     3421 tgcgcacgcc cgcggggcct tcagcgggcc tgttgcgcct tctgcccgcg cagcgcgccc
     3481 agttgctcct tgagcgtggt cagcgccgcg tgctggatcg ccccgcccac gttgccttcc
     3541 atccgcgcgg ccaggttcag ctggctcacg acctgcgaca tctcctccag ccaccagggc
     3601 tgcggcggcg gcgggttgtg gtgcagcagc caggcgatca gcacgctcag cggcacgctg
     3661 ccgcaggtgc cgtcgaacat ctccgaggcg aagccgctga tctggccgcc catctcggcg
     3721 acggcgccgc tgcgcgcctg cagcgagcgc ccgagctgga acagccgcac ctgctggaat
     3781 gcggccgcgg cggtgtcgcg cgcgatcggc ggcagcggct gcggattgat cgcgacttcg
     3841 gcttctcgcg tggcaatgat ctcctggatc gtgcccgcgc gcagcggacc gaagggccag
     3901 tgcggcggct tcgtgccgca gtagggatcg tcgtcgagcg cgctgacgaa ggcttgcagg
     3961 gtagctgcca tggaaggctc cttggttgag ggcagaggac cgcgacgatc ctctccgtcc
     4021 cgccaaggca ctatccctac aacgcggcat gcggcagcgc gcagcgctcg cctgcccggt
     4081 gcgcgtgacc cgcggcgccg gcatcccccc aggttcgtgg cgccgctgcc gttcgccgcg
     4141 ggccggccgc tcaggctcgc cggccggcgc gacgggcgtg cgccggcgcg cgcgtcacca
     4201 ccattcctcg cagtcgatgc ccgcgctgtc cagcgcgccg ccgcaggcca gcaccgagcc
     4261 gtcgggcatc agcgtggcgg tgggattgcc gcgcgccacc agcatctgcg acgcggccgt
     4321 ccactggccg gtggacgggt cgaacagctc ggcgccgggt tgcgcgaact gcgtgccgcc
     4381 gacgaccagg atgcgtccgt cgtggagcag cacggcgccg tgcgaggtgt gcgtggcggc
     4441 ggtgggcgac tgcgtcgtcc aggtggcggc gaccgggtcg tagatctccg tctgcgtgct
     4501 gccgccgccg atgaccacga cgcgtccgtc gccaaggcgc gtcgcgctgt ggccgttgcg
     4561 cgcgaaggcc agcggggtgg cggccgtgaa gtcctggccg gtgacgacag tggtgcttac
     4621 gccgttgacg accgtgttgg tggagtgcag caccgggtcg tacagctcgg cgctggccag
     4681 cggggtgagc acgccggcga cgatgtcgaa gccgccggcc acgagcacct tgccatcggc
     4741 cagcagcgtg gccgtgtgct gcgagcgcgc ggccagcggc atggcgcgcg gcgccgtcca
     4801 ggtgttggcg gcggggtcgt agatctcggc gctggcctcg accaggtagg ccgcgttctc
     4861 gccgcccacg accagcaccg tgccgtcggc cagcagcgtg gccgagtggt gcgagcgcgc
     4921 gacgctcatc ggcggcgtgg ccgtccacgt gttggcgacc gggtcgtaga tctcggcgct
     4981 ggcgttgttg acgtagccct gcgatgccga ggtcgacacc gtcgagccgc ccgtcaccag
     5041 cacgcggccg tcgggcagca gcgtggccgt gtgcgacgag cgcatcacgc ccatgggcgc
     5101 ggccggcgtc caggtggacg tggccgggtc gaacaggtcg gccgcggcgg tgccgccgct
     5161 ggacttcacg ccgcccgcca gcagcagccg gccgtcggcc agcagcgtgg cggtgtggcc
     5221 ggacacgcgc tcggtgggca tccggggcgc ggcggtccag gtgtcggtcg acaccgcgat
     5281 cacggcgccg gccgtgacgt tgccgatgcg ggccgtcacg ctggtggcgc cggccgccag
     5341 cccgatggcc gcgccgttgc tgaccgtggc gatgccgggc gtctgggtcg accaggtggc
     5401 gctggcggtg acgtcgccgg tgctgccgtc cgagaacgtg ccggtggccg tgaagtggcg
     5461 cgtgatgccg atgccggtga gcagcgaatc gggcgcgatg acgatggact tgaccgtggc
     5521 cgcgatcgtc gtgagcggca gcgtgccgct gacggcgccg gacgtggcgg tgatcgtcgt
     5581 gtcgcccagc gtcagcgcgt cggccaggcc cgtggcggcg ccgatgtcgg cgatggcggg
     5641 cgcgctcgac gtccaggtaa ccgcgcccga gatgtcggcc gagtggccgt ccgcgtaggt
     5701 gccggtggcg gtgaagcgct gcgagatgcc cagcggcagc gtgccggtgg tgggcgtgat
     5761 ggcgatggac gtcagcccct tgacgaaggt gacgcggccg atgcgcgcgc cgcccgcctg
     5821 caggtagatc ttcagcacgc cgtcggccag cgtgccgggc gcggcgagcg ccacggtgtc
     5881 gcccgcggcg agcgtgacgg cgccgctggc ggccgtgccg ttgaccgtga gctggcccgc
     5941 gctggcgctg atggccgcgc cggcatgcgc cgtgacgaag tcggccggca gcgtgaagct
     6001 cgcgcccgcc gcggcgccgt cgacgcggaa ctcgtcgcgc gcgacgacgc cgtcgccgtc
     6061 ctggtcgatc cactgggcga gctggttggc gtcgaagctg cggccgtaag tcgcggccag
     6121 gtgcgcggcg accgcgttga ggttgagcgc ctgttcgctg acggccagcg cctgctggct
     6181 ggcggcgctg atggcggcgc catgcgcgtt gatatcggct tgcagggacg cgagcagcgc
     6241 gccgacgtcc gccgaggcgc gtccctgcac gacgacgctg gacagcgccg ccagcaggct
     6301 gccgccgtcg gtgccggcgc cggcgtcgag ggtgccgaac gcgccgggcg aggcgggcag
     6361 cacgatgccg aacgcggcca ggacctcggt ctcggcctgc gcgcgggcgg cggcgaacgt
     6421 catgccgttg gtgttcagca gcgtgttggt gcgcgtgaag gccagcgtcg tcagcacgtt
     6481 gacgttgacg acctgctcgg ccttcaggtc ggcgtacgac tgcagcgtga ccgcgccgtc
     6541 ggacgactgc ccggtgacct cgtcggcgta gctgcccgag gccgtcagcg acaggtattg
     6601 cgaggcgtac ttgtcggacg gcgtgaacgc gccggtggcg tccgtggtgt agccgtagtg
     6661 gttgccggtg gccgacaggt tcagcccgag ctcctgcgcg gtgaccgtcg atccggcggc
     6721 catcggcccc ttctgggcga cgccggaggc cgccgtgtac gtggctgcgg gcggcacggg
     6781 cgtcgtgctg ccggtgggcg acccgccccc gcaggcggtg agcagcgcca tgagggcgag
     6841 cgatgcgacg ggcagcgggc agggcttcag gcggaacagt ggcatggcgc ggcggacttg
     6901 cagggcggtg acagggacgg ccgtgcgcgt ccagcgcgac agcaggcccg tggatttacg
     6961 catcgcgcgc cggaggcgtc aattggcgtt gccaacggtc acgggtgccg ggagcgcgtc
     7021 tgcgcgtgcg ggccccgcac atcgcgcgag ccgccgtgcg gccggcgagc ggccgtcggt
     7081 catcgggtcc ttccggggcg ctcgtcgcgc gcgagcctgc atcgggcatg tcgcgaaggc
     7141 ggcgagggag aatcgccgca tggccgacga atcccccgct cccgccgacg aatccccgga
     7201 cgccgccggc ccgcgccccg gcgacgcctt cgtgcagtcg ttcgcccgcg gcctggcggt
     7261 gctgcgcagc ttcggcgccg acgcgcccgc gcagacgctc acgagcgccg ccgagcgcag
     7321 cggcctcacg cgcgccggcg cgcggcgcat cctgctgacg ctgcagcagc tgggctatgt
     7381 cgagacggaa gggcggctgt tccgcctgac gccgcgcgtg atggagctgg gcttcgccta
     7441 cctcagctcg cagccggtgt ggcacctcgc gcagcccgtg atggaggagc tggtgcagga
     7501 catccacgaa tcgtcgtccg ccgcggtgct ggacggcgac gacatcgtct acgtgctgcg
     7561 cgtgccggcc aagaagatca tggcgatcag cctgggcccg ggcagccgcc tgccggcgta
     7621 ctgcacggcg atgggccgcg tgctgctggc cgggctgccg gagaccgagc gccgcgcccg
     7681 gctggagcgc gcgccgaggc ccgcgcgcac ggcacgcacc gtcaccgacg tcgatgcgtt
     7741 gctggccgtg ctggacggcg tgcgccgcga gggccacgcg ctggtgcacg gcgagctgga
     7801 ggagggcctg gtgtcgatgg ccgcgcccat cgtcaaccgc aacgggcgcg tcgtggcggc
     7861 gatcaacgtc agcgcgcagg accagcgcat gccgcccgac cagatgcgca agcgcctgct
     7921 gccgcggctg ctggaatcgg ccgtgagcat caacacgctg atgcgcatgc aggattgaag
     7981 cgactttccg cacgccggcc gcgttcgcgc gcggccgcgc cggcgccggg gggcgtgccg
     8041 cttggcagaa tcgccagttg cctgtcccga cccggcggac gccggctgcc cgcaaggcgc
     8101 cggaccgcc
//
LOCUS       DATCRO010000923         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_910195, whole genome shotgun sequence.
ACCESSION   DATCRO010000923 DATCRO010000000
VERSION     DATCRO010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910195"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1375
                     /locus_tag="VIN75_25060"
     CDS             260..1375
                     /locus_tag="VIN75_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977508.1"
                     /translation="MTDQASDKAPAQPDEGHWREIIGQIGTEVGLPLSAALERVTVLA
                     TTGKIDRAGLRMLREEIERARRAGMIGQQLARFASGRIRLSPEQVSVTQMLREVLLQR
                     GREAAARQVELSQVLKPAEVVADATLLHSLLQAVVDWSLDLAQGKLEFKLDIKAWPPY
                     ARISVRFPHASSDATDRLTPVGTPLPSLDTMAWRLVQQISWTLNLVLDRTVSDAETTL
                     TIEFPRTVHDEIEGVSVVEIDQGFGVSDNSKPLAGSHVLVVASTRDNRSLIREAIRHM
                     GLLVDFVGSVDEAEEFCRDALPHAIIFESLLANDRFQKLRNGIIAEMPTFVFIEITME
                     GSALQLSTQGNDGARIGRDAVMQSLPSALIFELSRTA"
     gene            complement(1396..2097)
                     /locus_tag="VIN75_25065"
     CDS             complement(1396..2097)
                     /locus_tag="VIN75_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY8977509.1"
                     /translation="MQRRQFTPALATVFLARASCLALLAEATRAFALDETEAASGVRA
                     ALERGADSAVSLLGKPGGFLDNPQVRIPLPGALERAASMLRAIGQGHRVDDLVNSMNR
                     AAEQAVPAARPLLLNAVHAMSVEDAVKIVRGGDTSVTDFFAAKTRAPLGEKFLPIVTT
                     ETQKVSLAAKYNAVASKGSAFGLVKPEDANVEQYVTRKALDGLYFMIGQEERKIRADP
                     VATGSALLKAVFGGR"
     gene            2109..2624
                     /locus_tag="VIN75_25070"
     CDS             2109..2624
                     /locus_tag="VIN75_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8977510.1"
                     /translation="MRFVRALAPLAFVLLASGAGLSTVMLAGCSRVEQAPAVSYTLLD
                     GRHGDLASLRGHVVLVNFWATDCAPCVEEMPAMVENWRRFSPQGFETLAVSMSYDAPA
                     LVSNFAQSRALPFPVVIDNTGEIAHRFGGVQYTPTSLLINKRGEIVRRWVGKTDFAEL
                     APLIARLDAQT"
     gene            complement(2632..>3473)
                     /locus_tag="VIN75_25075"
     CDS             complement(2632..>3473)
                     /locus_tag="VIN75_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8977511.1"
                     /translation="PDVALSGGLRLAAAAMPARARDDASTWIVPGLGTENPAAIARRL
                     AREDALRAMAALRRHVDRGGRVAASCSAVFLLQAAGLLAGRRATTSWWLAPELRRLEP
                     ACDVDADRMVCEDGPLTTAGAALAQSDLMLHLLRSRFGVALADAVGRVLLIDGRQAQA
                     PFALPAMMANGSELVRRLTRRIEAALPHPPSVAQLAAEQAMSSRTLARHVRAATGRGP
                     LALVQGVRLNHARHLIESSRLTIAQVAERVGYEDATALRRLMRKAAGAAPSRFRASWP
                     DPA"
BASE COUNT          492 a         1253 c         1215 g          513 t
ORIGIN      
        1 gtcaacgggt gtatccgaat gtttttcgtc gggcatcggg tcatcatggg agtggaagcg
       61 cggggcaacc gggccgtttt caccctcctg ggggatggaa ccagccctcg ccggtgggca
      121 atatgcggat catcaggatg cagtttgcta caatttgtaa ctggcgaaac gccgtttgcg
      181 cggtgcacgt ccggtgtcgc gcgccgcgcg cgcggaggga aagaacatgg caatgacttt
      241 ggcttcgagc cgctccatca tgaccgatca ggcttccgac aaggcgccgg cgcagccgga
      301 cgagggccac tggcgcgaga tcatcggcca gatcggtacg gaggtcggcc tgccgctgtc
      361 ggcggcgctc gaacgcgtca ccgtgctggc cacgaccggc aagatcgacc gggccggcct
      421 gcgcatgctg cgcgaggaga tcgaacgcgc ccggcgcgcc ggcatgatcg gccagcaact
      481 ggcccgcttc gcgtcgggcc gcatccgcct gtcgccggaa caggtcagcg tcacgcagat
      541 gctgcgcgag gtgctgctgc agcgcggccg cgaggccgcc gcccgccagg tcgagctgag
      601 ccaggtgctc aagcctgccg aggtcgtggc cgacgccacg ctgctgcact cgctgctgca
      661 ggcggtggtc gactggagcc tggacctggc gcagggcaag ctcgaattca agctggacat
      721 caaggcctgg ccgccctacg cccgcatcag cgtgcgcttc ccgcatgcca gttccgacgc
      781 caccgaccgc ctgacgccgg tgggcacgcc gctgccctcg ctcgacacga tggcctggcg
      841 cctcgtgcag cagatctcgt ggacgctgaa cctggtgctc gatcgcacgg tgtcggacgc
      901 cgagacgacg ctcacgatcg agttcccgcg caccgtgcac gacgagatcg agggcgtcag
      961 cgtcgtcgag atcgaccagg gcttcggcgt gtcggacaac tccaagccgc tggccggcag
     1021 ccacgtgctg gtcgttgcga gcacgcgcga caaccgctcg ctgatccgcg aggcgatccg
     1081 ccacatgggc ctgctggtcg acttcgtcgg cagcgtggac gaggccgagg agttctgccg
     1141 cgacgcgctg ccgcacgcga tcatcttcga gtcgctgctc gccaacgacc gcttccagaa
     1201 gctgcgcaac gggatcatcg cggagatgcc caccttcgtg ttcatcgaga tcacgatgga
     1261 aggcagcgcg ctgcagctgt cgacgcaggg caacgacggc gcgcgcatcg gccgcgacgc
     1321 ggtgatgcag tcgctgccgt cggcgctgat cttcgagctg tcgcgcaccg cctgagcgat
     1381 ccgccggcgg gcggctcagc gtccgccgaa caccgccttg aggagcgcgc tgccggtggc
     1441 caccgggtcc gcgcggatct tcctttcttc ctgcccgatc atgaagtaca ggccgtcgag
     1501 cgccttgcgc gtgacgtact gttcgacgtt cgcgtcctcg ggcttgacca ggccgaacgc
     1561 ggagcccttg ctcgcgacgg cgttgtactt ggcggcgagc gacaccttct gcgtctcggt
     1621 ggtgacgatc ggcaggaact tctcgcccag cggggcgcgc gtcttggcgg cgaagaaatc
     1681 ggtgaccgag gtgtcgccgc ctcgcacgat cttcactgcg tcctccaccg acatcgcgtg
     1741 cacggcgttc aggagcagcg gcctggcggc cggcaccgcc tgctccgcgg cgcggttcat
     1801 cgagttgacc aggtcgtcca cgcgatggcc ttgcccgatg gcgcgcagca tcgaggccgc
     1861 gcgttccagc gcgccgggca gcgggatgcg cacctgcggg ttgtcgagga agccgccggg
     1921 cttgcccagc agcgacaccg cgctgtcggc cccgcgctcg agcgcggcgc gcacgcccga
     1981 ggcggcctcg gtctcgtcga gcgcgaaggc cctggtggcc tcggccagca gcgcgaggca
     2041 ggacgcgcgg gcgaggaaca ccgtggccag ggccggcgta aactgacgtc gttgcatcgt
     2101 ggactccgat gagattcgtt cgcgccctcg caccgctcgc tttcgtgctg ctcgcttcgg
     2161 gcgccggtct gtcgaccgtg atgctagccg gttgcagccg ggtcgaacag gcacccgccg
     2221 tctcctacac cttgctggac ggccgccacg gcgacctcgc ctcgttgcgc ggccacgtgg
     2281 tgctggtcaa cttctgggcc accgactgcg cgccgtgcgt cgaggagatg cccgcgatgg
     2341 tggagaactg gcgccgcttc tcgccgcagg gcttcgagac gctggccgtc tcgatgtcgt
     2401 acgacgcgcc ggcgctcgtc tcgaacttcg cgcagtcgcg cgcgctgcct tttcccgtcg
     2461 tcatcgacaa caccggcgag atcgcgcatc gcttcggcgg cgtgcagtac acgcccacgt
     2521 cgctgctcat caacaagcgc ggcgagatcg tgcggcgctg ggtcggcaag accgacttcg
     2581 ccgaactggc gccgctgatc gcgcgcctcg acgcccagac ctgagccttg ctcaggccgg
     2641 gtccggccat gacgccctga agcggctcgg cgccgcgccg gccgccttgc gcatcaggcg
     2701 ccgaagcgcc gtcgcgtctt cgtagccgac gcgttcggcc acctgcgcga tcgtcaaccg
     2761 gctcgactcg atcaggtggc gcgcgtggtt caggcgcacg ccctgcacca gcgccagggg
     2821 gccgcgtccg gtcgccgcgc gcacgtgacg cgcgagcgtg cgcgaggaca tcgcctgctc
     2881 cgcggccagc tgcgcgacgc tgggcggatg gggcagggcc gcctcgatgc gccgcgtcag
     2941 ccggcgcacg agctcgctgc cgttggccat catggccggc agcgcgaacg gcgcctgcgc
     3001 ctggcggccg tcgatcagca gcacccgacc cacggcatcg gccagcgcca cgccgaagcg
     3061 gctgcgcagc aggtgcagca tcaggtccga ctgcgccagc gccgcgcccg cggtcgtcag
     3121 cgggccgtcc tcgcacacca tgcggtcggc gtcgacgtcg cacgccggct ccaggcggcg
     3181 cagctccggc gccagccacc aggacgtggt cgcgcgccgg ccggccagca ggcccgcggc
     3241 ctgcagcagg aacacggccg aacacgacgc cgccacgcgg ccgccgcgat cgacatggcg
     3301 ccgcagcgcc gccatcgcgc gcagcgcgtc ctcccgcgcc aggcgccggg cgatcgccgc
     3361 ggggttctcg gtgcccaggc ccggcacgat ccacgtggaa gcgtcgtcac gcgcgcgggc
     3421 cggcatcgcc gctgccgcca ggcgcaggcc gccgctgagc gccacgtccg gac
//
LOCUS       DATCRO010000924        10091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_11202, whole genome shotgun sequence.
ACCESSION   DATCRO010000924 DATCRO010000000
VERSION     DATCRO010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11202"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VIN75_25080"
     CDS             <1..345
                     /locus_tag="VIN75_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977512.1"
                     /translation="EEVQRVLHEIGADEVPQVLVYNKLDRLDVAQRPESLVDEIEGDR
                     GLRTPRVFVSALNGEGLDALRQAIADALIASRDAPEEDFERDARFSRPDLDDNAGDDG
                     FQNPLASPSSSA"
     gene            342..1613
                     /gene="hflK"
                     /locus_tag="VIN75_25085"
     CDS             342..1613
                     /gene="hflK"
                     /locus_tag="VIN75_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY8977513.1"
                     /translation="MNMPENTSPTSVPPARRRSLLRKLLGVFMAQNPEGPPDLEEMWR
                     DFTRKLSSFFGVGKRGGGGGGDPGNGFQPDARSAGMGVMAIALIVVVVWLFSGSFIVQ
                     EGQQAVVTTFGRYSHTVGAGWGWRWPYPIQANETVNFTQTTTVEIGAGAGSSSAAMSD
                     SAMLTQDENIVDVRFSVQYNLKDARAYLYENNNPKLAVTQAAESAVREIVGNSRIDSV
                     LYEQRAALTASLVKSIQQQLDALNAGILIVNVNIEAVNPPDPVQQAFSDALKAGVDRE
                     ALKNEGQAYANRVIPAAQGDAARLREDALAYKARIVNAAQGDSDRFKAVYAEYQKAPA
                     VTRDRMYIDAMQAVYSNVTKVMVDTHNGTNLIQLPLDKLAQPSAGAAPAPAAPSPTDA
                     SAAAATVTVTPNDANDARSRDNARSRDRDTR"
     gene            1626..2534
                     /gene="hflC"
                     /locus_tag="VIN75_25090"
     CDS             1626..2534
                     /gene="hflC"
                     /locus_tag="VIN75_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY8977514.1"
                     /translation="MNRIFLAIGIAIIAALVASSSLFIVDQRKVAVIKTFGEIKSVIT
                     EPGLNFNWPLVQTVSMLDKRIQTLDNPTPGPIFTAEKKSLVIDWLVKWRINDARQFIR
                     NNSGEQRNLEIRLAAVTQATFNEEITKHTVRGVLSDERAQIMKNVKDRLEVESKSFGI
                     DIIDVRIKRVDFVPEVTTSVYNRMKAERAQVANLARSEGGAIKEKISADADKQVAVIL
                     SEAQLDAQKTKGDADAQATQIYGEAFGRDPKFAQFYRNLEAYRASFNKKSDVVVVDSS
                     SDFFKAMRGSGTGAGGTADAAAAKKH"
     gene            2536..2727
                     /locus_tag="VIN75_25095"
     CDS             2536..2727
                     /locus_tag="VIN75_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY8977515.1"
                     /translation="MTLLILVSAVALVLILEGLLPFLNPGAWRRVFERALQLSDAQIR
                     LLGLSSLLAGTLILLVFGP"
     gene            2886..4058
                     /locus_tag="VIN75_25100"
     CDS             2886..4058
                     /locus_tag="VIN75_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY8977516.1"
                     /translation="MTSAWLLPEHIADVLPSEARRIEELRRKLLDLARSYGFELVIPP
                     MLEHLESLLSGAGLGHDLKTFKLVDQLSGRMLGVRADTTPQVARIDAHLLNRQGVARL
                     CYCGPVLHARPDSPLATREPLQFGAEIYGHAGLEADLEIQDLALDGLAAAGVGRLVLD
                     MGDARIVRSLLAGEGTPADSVADIVAALTLKDRSAVETLVAGCSAPVRGALLTLLDLY
                     GGDDVLAVARERLPALPGITAALDDLAWLGRHLRAAHPDIEVGFDLSDMSGYAYYSGV
                     RFSVYAAGSTDALLRGGRYDHVGAVFGRNRPAVGFSLDLRQLSAAMAAAPLRAAIRAP
                     WSEDAALRAAIRALRAAGETVVCALPGHEHETQEFDCDRELVVIDNQWTLRSLTTA"
     gene            4120..5460
                     /locus_tag="VIN75_25105"
     CDS             4120..5460
                     /locus_tag="VIN75_25105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8977517.1"
                     /translation="MQQGNTTIPSRGRNVVVVGTQWGDEGKGKVVDWLTDHAEHVVRF
                     QGGHNAGHTLVIKGVKTALQLIPSGVMRDGVNCYIGNGVVVDPSHLLAEIERLEKIGL
                     DVRSRLYLSEACPLIMPFHVAVDKAREAQREADGSGKIGTTGKGIGPAYEDKVARRAL
                     RVQDLKHPERFAGKLRELLDVHNATLQRLGAEQLEFAPILDQAMKAAEAIKPMIADVG
                     YALYKAHQAGAKILFEGAQGTLLDIDHGTYPFVTSSNCVAGNAAAGTGLGPGMLHYIL
                     GITKAYTTRVGSGPFPTELDIHQEGGIGHHLSVVGQERGTVTGRARRCGWLDAAALKR
                     AIIINGISGLCITKLDVLDGLDEIKVCIGYKLDGQDVDILPSDADDVERCEPVYESYP
                     GWTEKTFGLTQWDQLPLNARRYLERVKELIGAPIDMVSTGPDRDHTILLRHPYR"
     gene            5595..6122
                     /locus_tag="VIN75_25110"
     CDS             5595..6122
                     /locus_tag="VIN75_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY8977518.1"
                     /translation="MLTDDGKHLYISWNEYHELIERLALKVHASGWQFDQILCLARGG
                     MRPGDMLSRIFDKPLAIMSTSSYRAEAGTIQGRLDMAKYITLPRGELAGRVLLVDDLA
                     DSGVTLAAVAERLRGLPAIQELRSAVLWTKAVSTYTPDYFVEVLPTSPWIHQPFEDYD
                     NISIETLIKRHSTED"
     gene            6236..6817
                     /locus_tag="VIN75_25115"
     CDS             6236..6817
                     /locus_tag="VIN75_25115"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY8977519.1"
                     /translation="MQAQTDRRRLALAFAANVAMFAVGLVGWRFAHSAALLGDALDML
                     ADASGYAIAWLAVGAGAGRQRAAARWNGAMLMALGVALFGEVAHRWFVGEAPDGPWIA
                     VFAALSLAVNGGVLRLLSAYRDSRQPHLRATWIDTRADVLVNVGVLVSGVLVTLTAGR
                     AIDLVAGAVLGVFVIHEGWEIWEGDDEDDDDDA"
     gene            6807..7433
                     /locus_tag="VIN75_25120"
     CDS             6807..7433
                     /locus_tag="VIN75_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8977520.1"
                     /translation="MTHDCSHLIGTLAELEALLGPVGEASLRKETTTLHPHYQAMIQA
                     SPFALLATSGPGGLDASPRGDPPGFVQVHDERTLLMPERRGNNRADSLRNIVGDPRVG
                     LLFLIPGVGETLRVNGRATISVDPALLERFRMQGQLPRCVLVIAVETAFFQCARAITR
                     SNLWRALPADAPRPVPTPGAMLEALTQAQIDGATYDRDLPERQRTTLY"
     gene            complement(7599..7683)
                     /locus_tag="VIN75_25125"
     tRNA            complement(7599..7683)
                     /locus_tag="VIN75_25125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7647..7649),aa:Leu,seq:cag)
     gene            7826..10048
                     /gene="rnr"
                     /locus_tag="VIN75_25130"
     CDS             7826..10048
                     /gene="rnr"
                     /locus_tag="VIN75_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY8977521.1"
                     /translation="MGEAEGVVVGHRDGHGFVKRDDGEPDVYISQQEMRSVLHRDRVR
                     VRVVRFDRKGRPEGRILEIVERRKAPIIGRLLNEGGAWLVAPEDRRYGQDILIPKNAT
                     ASAVAGQVVAVELTEPPSMYSQPVGRITEVLGEIDDPGMEIEIAVRKYEVPYRFSAPT
                     LAQTAKLPDHVRAADMKGRINLTDVPLVTIDGEDARDFDDAVYCEPHKSGRGKNAFTG
                     WRLIVAIADVSHYVKPGEALDTDAYERATSVYFPRRVIPMLPEKLSNGLCSLNPEENR
                     LSMVCDMLVGEDGELGPYQFYPATICSHARLTYTEVAAVLGNTRGPEAAERADLVPHL
                     LNLHDVYRALLKARTRRGAIDFETTETQIVCDDNGRIERIVPRTRNDAHKLIEEAMLA
                     ANVCAADFIALHEHPALFRVHEGPTPEKKVTLQNYLKALGLGLSISDDPKPAEFQQLA
                     QATKDRVDAQQIHSMLLRSMQQAIYTPSNSGHFGLAYQAYAHFTSPIRRYPDLLVHRV
                     IKAILHGKKYHLANPEVENSAAEGLRLGRANVGAKVSHSEIAQWVAAGEHCSANERRA
                     DEASRDVEAWLKCRFMRERLGEEFSGTVSAATTFGLFVTLDSLYVEGLVHITELGGEY
                     FRFDEVRQELRGERTGIRYAIGARVRVQVSRVDLDGRKIDFRIVREAEGERLLSRGRK
                     TEPRAGVATAAEQLASVEAQDRDVKRAARKTKAGARAAKGAAAKTSTRGKPVAKRSKR
                     "
BASE COUNT         1655 a         3606 c         3413 g         1417 t
ORIGIN      
        1 gaggaggtgc agcgcgtgct gcacgagatc ggcgccgacg aggtgccgca ggtcctcgtc
       61 tacaacaagc tcgatcgcct ggacgtcgct cagcgccccg agtcgctggt cgacgagatc
      121 gaaggtgacc gcgggctgcg cacgccgcgc gtgttcgtca gcgccctgaa cggcgagggg
      181 ctggacgcgc tgcgccaggc catcgccgac gccttgatcg ccagccgcga cgcgcccgag
      241 gaggacttcg agcgcgacgc acgcttttcc cggcccgacc tggacgacaa tgccggcgac
      301 gacggcttcc aaaatccgct ggcgtcccca tcttcttccg catgaacatg cccgagaaca
      361 cctccccgac atcggtgccg cccgcgcgcc ggcgctcgtt gctgcgcaaa ctcctgggcg
      421 tcttcatggc gcagaacccg gaagggccgc ccgacctcga ggagatgtgg cgtgacttca
      481 cccgcaagct ctcgtcgttc ttcggcgtgg gcaagcgcgg cggcggtggc ggcggcgacc
      541 cgggcaacgg cttccagccg gatgcccgca gcgccggcat gggtgtcatg gccatcgcgc
      601 tgatcgtggt ggtcgtgtgg ctgttcagcg gctcgttcat cgtgcaggaa ggccagcagg
      661 ccgtcgtcac caccttcggc cgctacagcc acacggtggg cgccggctgg ggctggcgct
      721 ggccgtaccc gatccaggcc aatgagacgg tcaacttcac gcagaccacg acggtggaga
      781 tcggcgcggg cgccggctcc tcgtccgcgg ccatgtccga ttcggccatg ctgacgcagg
      841 acgagaacat cgtcgacgtg cgcttcagcg tgcagtacaa cctgaaggac gcgcgggcct
      901 acctgtacga gaacaacaac ccgaagctgg cggtcacgca ggccgccgag tcggcggtgc
      961 gcgagatcgt cggcaacagc cgcatcgact ccgtgctgta cgagcagcgc gccgcgctga
     1021 cggcgtcgct ggtcaagtcg atccagcagc agctggacgc gctcaacgcc ggcatcctga
     1081 tcgtcaacgt caacatcgag gcggtcaatc cgcccgatcc ggtgcagcag gcgttcagcg
     1141 acgccctcaa ggccggcgtc gatcgcgagg cgctcaagaa cgagggccag gcctacgcca
     1201 accgcgtgat ccccgcggcg caaggcgatg ccgcgcgcct gcgcgaggat gcgctggcgt
     1261 acaaggcacg catcgtcaac gcggcgcagg gcgactccga ccgcttcaag gcggtctacg
     1321 ccgagtacca gaaggcgccg gccgtcacgc gcgaccgcat gtacatcgac gcgatgcagg
     1381 cggtgtactc caacgtcacg aaggtgatgg tcgacacgca caacggcacg aacctcatcc
     1441 agttgccgct ggacaagctc gcgcagccgt ccgccggcgc cgcgcccgcg ccggccgcgc
     1501 cgtcgccgac cgacgcgagc gccgccgccg cgacggtcac cgtgacgccc aacgatgcca
     1561 acgacgcccg ctcgcgcgac aacgcccgca gccgcgaccg cgacacccgc tgaggagccg
     1621 acgccatgaa tcgaatcttc ctcgcgatcg gcatcgcgat catcgccgcc ctggtggcct
     1681 cgtcgtcgct gttcatcgtc gaccagcgca aggtggccgt catcaagacc ttcggcgaga
     1741 tcaagtccgt catcaccgag ccgggcctga acttcaactg gccgctggtg cagaccgtct
     1801 cgatgctgga caagcgcatc cagaccctgg acaacccgac gcccggcccg atcttcacgg
     1861 ccgagaagaa gagcctggtc atcgactggc tggtgaagtg gcgcatcaac gacgcgcgcc
     1921 agttcatccg caacaactcc ggcgagcagc gcaacctcga gatccgcctg gcggccgtca
     1981 cgcaggcgac gttcaacgag gagatcacca agcacacggt gcgcggcgtg ctgtccgacg
     2041 aacgtgccca gatcatgaag aacgtcaagg atcgcctgga ggtggaatcc aagtcgttcg
     2101 gcatcgacat catcgacgtg cgcatcaagc gggtggactt cgtgccggag gtcaccacct
     2161 cggtctacaa ccgcatgaag gccgagcgcg cccaggtggc caacctggcg cgttccgagg
     2221 gcggcgccat caaggagaag atcagcgccg atgccgacaa gcaggtcgcg gtgatcctgt
     2281 ccgaggccca gctcgacgcg cagaagacca agggcgacgc ggacgcgcag gccacgcaga
     2341 tctacggcga ggccttcggc cgcgacccga agttcgccca gttctaccgc aacctcgagg
     2401 cctatcgcgc cagcttcaac aagaagtcgg acgtcgtggt ggtcgattcc agctccgact
     2461 tcttcaaggc catgcgcggc tccggtaccg gcgccggcgg gacggccgac gcggccgccg
     2521 ccaagaagca ctgagatgac gttgctgatc ctcgtcagcg ccgtcgcgct ggtgttgatc
     2581 ctcgagggcc tgctgccgtt cctgaacccc ggtgcctggc gccgggtgtt cgagcgggcc
     2641 ctgcagctga gcgacgcgca gatccggctg ctggggctgt ccagcctgct cgccggcacc
     2701 ctgatcctgc tcgttttcgg gccctgaccc cccgggccgg cgcccgccgc gccccgccgc
     2761 cgcgaaggct ggcttgcccg aatgcccgtt gctggcagcc gccagcgtgc cgccccgcac
     2821 gaaacaatcg tctgcggtgc gggacacatc ggtacaatcc tggttttaac ccctccagca
     2881 ttctcatgac gtctgcttgg ctactgcccg agcacatcgc cgatgtcctg ccgtccgaag
     2941 cgcggcgcat cgaggaactg cggcgcaagc tgctcgacct ggcccgcagc tacggattcg
     3001 agctggtgat cccgccgatg ctggagcacc tggagtcgct gctgtcgggc gccggcctcg
     3061 ggcacgacct caagaccttc aagctggtcg accagctgag cggccgcatg ctgggcgtgc
     3121 gcgccgacac caccccgcag gtcgcccgca tcgacgccca cctgctcaat cgccagggcg
     3181 tcgcccgcct gtgctactgc ggcccggtgc tgcacgcgcg cccggacagc ccgctggcca
     3241 cccgcgagcc gctgcagttc ggcgccgaga tctacggcca cgccggcctc gaggccgacc
     3301 tggagatcca ggacctggcc ctggacggcc tggccgccgc cggcgtcggc cgccttgtcc
     3361 tggacatggg cgatgcgcgc atcgtgcgca gcctgctcgc cggcgagggc acgcccgccg
     3421 acagcgtggc cgacatcgtc gccgcgctga cgctcaagga tcgcagcgcc gtcgagacgc
     3481 tggtcgcggg ctgctccgcg ccagtgcgcg gggcgctgct gacgctgctc gatctgtacg
     3541 gcggcgacga cgtgctggcc gtcgcgcgcg agcgcctgcc cgcgctgccc ggcatcaccg
     3601 cggcgctcga cgacctcgcc tggctgggcc ggcacctgcg cgcggcgcac ccggatatcg
     3661 aggtcggctt cgacctgtcc gacatgagcg gctacgccta ctacagcggc gtgcgcttct
     3721 ccgtctacgc cgcgggctcg accgacgcgc tgctgcgcgg cggccgctac gaccacgtcg
     3781 gcgcggtgtt cggccgcaac cgtccggccg tgggcttcag cctcgacctg cgccagctgt
     3841 cggccgccat ggcggccgcg ccgctgcgcg cggccatccg cgcgccgtgg agcgaggacg
     3901 ccgcgctgcg cgccgccatc cgcgcgctgc gcgccgcagg cgagaccgtg gtctgcgcct
     3961 tgccgggcca cgagcacgag acgcaggaat tcgactgcga ccgcgagctc gtcgtcatcg
     4021 acaaccagtg gacgctgcgc agtctgacga ccgcgtagcc cgccgcctcc gcaccccccg
     4081 gtcgccacgg ccgccgaatc actcctctcc cgaagttcca tgcaacaagg caacaccacc
     4141 atcccctcgc gcggccgcaa cgtcgtcgtc gtcggcaccc aatggggcga cgaaggcaag
     4201 ggcaaggtcg tcgactggct gaccgaccac gccgagcacg tcgtgcgctt ccagggcggc
     4261 cacaacgcgg gccacacgct ggtcatcaag ggcgtcaaga cggcgctgca gctgatcccg
     4321 tcgggcgtga tgcgcgacgg cgtgaactgc tacatcggca acggcgtggt ggtcgatccg
     4381 tcgcacctgc tggccgagat cgagcgcctg gagaagatcg gcctggacgt gcgctcgcgc
     4441 ctgtacctga gcgaggcctg cccgctgatc atgccgttcc acgtggccgt ggacaaggcg
     4501 cgcgaggcgc agcgcgaggc cgacggcagc ggcaagatcg gcaccacggg caagggcatc
     4561 ggcccggcct acgaggacaa ggtcgcccgc cgcgcgctgc gcgtgcagga cctgaagcac
     4621 ccggaacgct tcgccggcaa gctgcgcgag ctgctggacg tgcacaacgc gacgctgcaa
     4681 cgcctgggcg ccgagcagct ggagttcgcg cccatcctcg accaggccat gaaggccgcc
     4741 gaggccatca agccgatgat cgctgacgtc ggctacgcgc tctacaaggc ccaccaggcc
     4801 ggcgccaaga tcctgttcga gggcgcgcag ggcacgctgc tggacatcga ccacggcacc
     4861 tacccgttcg tcacctccag caactgcgtg gccggcaacg ccgccgccgg cacgggcctg
     4921 ggcccgggca tgctgcacta catcctgggc atcaccaagg cctacacgac gcgcgtcggc
     4981 tcgggtccgt tcccgaccga gctggacatc catcaagagg gcggcatcgg ccaccacctg
     5041 tcggtcgtcg gccaggagcg cggcaccgtc accggccgcg cccgccgctg cggctggctc
     5101 gacgccgcgg cgctcaagcg cgcgatcatc atcaacggca tctccggcct gtgcatcacc
     5161 aagctggacg tgctggacgg cctggacgag atcaaggtct gcatcggcta caagctcgac
     5221 ggccaggacg tcgacatcct gcccagcgat gcggacgacg tcgagcgctg cgagcctgtc
     5281 tacgagagct accccggctg gacggagaag accttcggcc tgacgcagtg ggaccagctg
     5341 ccgctcaacg cgcgccgcta tctcgagcgc gtcaaggaac tgatcggcgc gccgatcgac
     5401 atggtctcga ccggaccgga ccgcgaccac acgatcctcc tgcgccaccc gtaccgctga
     5461 aaggcgtcgg cgccggctcg ctgggcacgc cggcgtcgaa gatccgttgc actgacaccc
     5521 gaaccccgtt ccgcaacgag gggcgcccac gaagcgctgc ggagcggctt gaacagaacc
     5581 actgaaggaa tgcgatgctc accgacgacg gcaagcacct gtacatctcc tggaacgaat
     5641 accacgaact gatcgaacgc ctggcgttga aggtgcacgc ctcgggctgg cagttcgacc
     5701 agatcctgtg cctggcgcgc ggcggcatgc gcccgggcga catgctctcg cgcatcttcg
     5761 acaagccgct ggccatcatg tccaccagct cgtatcgcgc cgaggccggc acgatccagg
     5821 gccggctcga catggccaag tacatcacgc tgccgcgcgg cgaactggcg ggccgcgtgt
     5881 tgctggtcga cgacctggcc gattccggcg tgaccctggc cgcggtcgcc gagcgcctgc
     5941 gcgggctgcc agcgatccag gaactgcgct ccgcggtgct gtggaccaag gcggtctcca
     6001 cctacacccc cgactacttc gtcgaggtgc tgcccaccag cccctggatc caccagccgt
     6061 tcgaggacta cgacaacatc tcgatcgaga cgctgatcaa gcggcactcg accgaggact
     6121 gagcccgcgg gccgccgccc aggcggcttc gcgattgcaa ggggctcgct ttccgcgggc
     6181 cccttgtcgt tcgatgcgcg acctgcggga gccaggacac gtggacgatc cggacatgca
     6241 ggcccagacc gaccgccgcc gcctggcgct ggccttcgcc gcgaacgtgg cgatgttcgc
     6301 cgtcggcctc gtcggctggc gcttcgcgca ctcggcggcg ctgctgggcg acgcgctcga
     6361 catgctggcc gacgcgtccg gctacgcgat cgcctggctc gccgtgggcg ccggcgcggg
     6421 tcgccagcgg gccgcggcgc gctggaacgg cgcgatgctg atggcgctcg gcgtggcgct
     6481 gttcggcgag gtggcgcatc gctggttcgt cggcgaggcg cccgacgggc cctggatcgc
     6541 cgtgttcgcg gcgctgtcgc tcgcggtcaa cgggggggtg ctgcggctgc tgtcggccta
     6601 cagggacagc cgccagccgc acctgcgcgc cacgtggatc gacacgcgcg ccgacgtgct
     6661 cgtcaacgtc ggcgtgctgg tgtcgggcgt gctggtgacg ctgacggccg ggcgcgccat
     6721 cgacctcgtc gccggcgcgg tgctcggggt gttcgtcatc cacgagggct gggagatctg
     6781 ggaaggcgat gacgaggacg acgacgatga cgcatgactg ctcccacctc atcgggacgc
     6841 tggccgaact ggaggccctg ctgggcccgg tgggcgaggc gtcgttgcgc aaggagacga
     6901 cgacgctgca tccgcattac caggcgatga tccaggcctc gccgttcgcg ctgctggcca
     6961 cgagcgggcc gggtggcctg gatgcgtcgc cgcgcggcga tccgccgggc ttcgtgcagg
     7021 tgcacgacga gcgcacgctg ctgatgcccg aacgccgcgg caacaaccgc gccgacagcc
     7081 tgcgcaacat cgtgggcgac ccgcgcgtgg gcctgctgtt cctgattccc ggcgtgggcg
     7141 agacgctgcg cgtgaacggg cgcgcgacga tcagcgtcga ccccgcgctg ctcgagcgtt
     7201 tccgcatgca gggccagttg cccaggtgcg tgctcgtgat cgccgtggag acggcgttct
     7261 tccagtgcgc gcgggcgatc acgcgatcga acctgtggcg cgcgctgccg gccgatgcac
     7321 cgcgtccggt gcccacgccc ggcgcgatgc tcgaggcgtt gacgcaggcg cagatcgacg
     7381 gcgcgacgta cgaccgcgac ctgccggagc gccagcgcac gacgctgtat tgaggcgcgc
     7441 agggcacctg gcgtgcgcac gcgtcgcgcg gccgcgcatc gcgcgtgcag gcgatgcgct
     7501 tgcgactgca cgcatcgtcc ccatgtgtga tgcgtgcgtg ccacgcgcgg aaaagaaaaa
     7561 agccccgccg accgaagtct gcggggcttt ctcgaatatg gtgcccagaa gaggactcga
     7621 acctccacgc cgttaagcgc tagtacctga aactagtgcg tctaccaatt ccgccatctg
     7681 ggcaaggtac atttcgttca ttgaacttcg cggccgtatc gagcactttc gtttcgattc
     7741 gtcgagctgt tcaatcagcg aagaaagaga tcatatcatc aaaaacaaaa cagttgcaag
     7801 cccccccgcg ctcaacggcg cattgatggg cgaagccgaa ggagtggtgg tcggtcatcg
     7861 tgacggccac gggttcgtca agcgggacga cggcgaaccg gacgtctaca tctcgcagca
     7921 ggagatgaga tccgtgctgc accgcgaccg cgtcagggtg cgcgtcgtgc gcttcgaccg
     7981 caagggccgg cccgaagggc gcatcctcga gatcgtcgag cggcgcaagg cgcccatcat
     8041 cggccgcctg ctcaacgagg gcggcgcctg gctggtggcc cccgaggatc gccgctacgg
     8101 ccaggacatc ctgatcccca agaacgccac ggcgtccgcg gtcgcgggcc aggtggtggc
     8161 ggtcgagctc accgagccgc cgtcgatgta ttcgcagccc gtgggccgca tcaccgaggt
     8221 gctgggcgag atcgacgacc ccggcatgga gatcgagatc gcggtgcgca aatacgaggt
     8281 gccgtatcgg ttctcggcgc ccacgctcgc gcagacggcc aagctgcccg accacgtgcg
     8341 agcggccgac atgaagggcc gcatcaacct gaccgacgtg ccgctggtca ccatcgacgg
     8401 cgaagacgcg cgcgacttcg acgacgccgt ctactgcgag ccccacaagt cgggccgcgg
     8461 caagaacgcc ttcacgggtt ggcgcctgat cgtcgcgatc gccgacgtca gccactacgt
     8521 caagcccggc gaggcgctgg ataccgacgc gtacgagcgc gcgacgtcgg tgtacttccc
     8581 gcgccgcgtg atcccgatgc tgcccgagaa gctgtccaac ggcctgtgct cgctcaatcc
     8641 cgaggagaac cgcctctcga tggtgtgcga catgctcgtc ggcgaggacg gcgagctggg
     8701 tccgtaccag ttctacccgg ccacgatctg ctcgcacgcg cggctgacgt acaccgaggt
     8761 ggcggcggtg ctgggcaaca cgcgcggccc cgaggcggcc gagcgcgccg acctggtgcc
     8821 gcacctgctg aacctgcacg acgtctaccg cgccctgctc aaggcacgca cgcgccgcgg
     8881 cgcgatcgac ttcgagacga ccgagacgca gatcgtctgc gacgacaacg gccgcatcga
     8941 gcgcatcgtg ccgcgcacgc gcaacgacgc gcacaagctg atcgaggagg cgatgctggc
     9001 ggccaacgtc tgcgccgccg acttcatcgc actgcacgag cacccggcgc tgttccgcgt
     9061 gcacgagggc ccgacgccgg agaagaaggt cacgctgcag aactacctca aggcgctcgg
     9121 cctggggctg tcgatcagcg acgacccgaa gccggccgag ttccagcagc tcgcgcaggc
     9181 caccaaggac cgcgtcgacg cgcagcagat ccactcgatg ctgctgcgct cgatgcagca
     9241 ggccatctac acgcccagca acagcgggca cttcggcctc gcgtaccagg cctacgcgca
     9301 cttcacgagc ccgatccgcc gctaccccga cctgctggtg catcgcgtga tcaaggcgat
     9361 cctgcacggc aagaagtacc acctggccaa tccggaggtc gagaactcgg ccgccgaagg
     9421 cctgcgcctg ggccgggcca atgtcggcgc caaggtctcg cattcggaga tcgcgcaatg
     9481 ggtggccgcc ggcgagcact gcagcgccaa cgagcgccgc gccgacgagg cgtcgcgcga
     9541 cgtcgaggcc tggctcaagt gccgcttcat gcgcgagcgc ctgggcgagg agttctccgg
     9601 caccgtcagc gcggcgacga ccttcgggct gttcgtcacg ctcgactcgc tctacgtcga
     9661 gggcctggtg cacatcaccg agctcggcgg cgagtacttc cgcttcgacg aggtgcgcca
     9721 ggagctgcgc ggcgagcgca cgggcatccg ctacgcgatc ggcgcgcggg tgcgggtgca
     9781 ggtcagccgc gtcgacctgg acggccgcaa gatcgacttc cgcatcgtcc gcgaagccga
     9841 gggcgagcgc ctgctcagcc gcggccgcaa gaccgagccg cgcgcgggcg tggcgacggc
     9901 ggccgagcag ctggcgtcgg tcgaggcgca ggatcgcgac gtcaagcggg ccgcgcgcaa
     9961 gaccaaggcc ggcgcccggg cggccaaggg ggcggcggcc aagacctcga cgcgcggcaa
    10021 gcccgtcgcc aagcgatcca agcgctgagg ccgtcccgta cttgagcagg agaggccccg
    10081 tgttccgggg c
//
LOCUS       DATCRO010000925        10397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1000480, whole genome shotgun sequence.
ACCESSION   DATCRO010000925 DATCRO010000000
VERSION     DATCRO010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000480"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VIN75_25135"
     CDS             complement(<1..660)
                     /locus_tag="VIN75_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977522.1"
                     /translation="MDRALLLHLPIVLAVHRRGGFAAAANDLGLSPSAVSHAVQLVEA
                     GLGQPLFARTTRSVRLTEAGAAFVASIGPALAEIEEAAERLGADQGRVSGTLRLNSPR
                     TALPIAVTPVLAELARRHPGLTVEVTADDGLADIVAQGYDAGIRLGHTVAQDMVAVRL
                     TPPFHSIMVASPAYLADRGMPRRLDDLDRHNCIGYRFVTSGAVYDWDLRDGGLAVAKA
                     VG"
     gene            776..1618
                     /locus_tag="VIN75_25140"
     CDS             776..1618
                     /locus_tag="VIN75_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY8977523.1"
                     /translation="MNTTAQTWFITGSSSGFGRAFAEHAIAQGHNVVATARRVDALAD
                     LVAQAPGRVLALALDVDVARDAEAAVGAAVARFGRIDVLVNNAGYGVVGALEETPDAE
                     LRALFDTNFFGALALTRAALPVLREQRGGAIVNMSSLGGQLSFAGFSAYSATKFALEG
                     ASEALAQEVAPFGIKVLIVEPGQFRTQLAGAGMRHMPELEAYAATVGGTRTFAHDMHQ
                     TQAGDPRKAAVAIERALAAPVTPLRLQLGGDSVDAVRAHAQALLADLQAWEAVARGTD
                     FDAR"
     gene            complement(1622..2944)
                     /gene="chrA"
                     /locus_tag="VIN75_25145"
     CDS             complement(1622..2944)
                     /gene="chrA"
                     /locus_tag="VIN75_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8977524.1"
                     /translation="MPPRREAFAFWLKLGFISFGGPAGQIAVMHEELVERRRWISEQR
                     FLHALNYCMLLPGPEAQQLATYVGWLMHRTIGGIVAGGLFVLPSLFILIGLSWVYMAF
                     GQVPTIAGLFHGIKPAVTALVLHAAWRVGSRTLTNAWLWGIAVASFVAIFVLHLPFPL
                     IVLLAALAGALGGWLAPDKFRPGPARPGASGSHAPALIDDATPVPAHARHAWPRALRI
                     VAAFAALWGGALAALAAATGWNGTLAQMAWFFTKTAWLGFGGAYAILPYVMQGGVDHY
                     HWLTAAQMMDGLALGETTPGPLIMVVAFVGYVGGWTQAVFGPGALALAGAAAATVVTF
                     FTFLPSFFFILVGGPVIESTHGNLKFTAPLTGVTAAVVGVIVNLAAFFALHVLWPHGP
                     GGRFDAAALAIGIAAAVALFRLRAGVIPVIAGCALAGLLLTLAGVTAR"
     gene            3073..3423
                     /locus_tag="VIN75_25150"
     CDS             3073..3423
                     /locus_tag="VIN75_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8977525.1"
                     /translation="MTISAISKPDVLAALAVLAHPVRLDVFRVLVQAGPVGLTPTALA
                     ALVQPELRQSTLATHLKELVTAGLVANARAGRNVVYRADYERMGGVIAYLTENCCAGA
                     PCAVEPTCQSACGC"
     gene            3442..3924
                     /locus_tag="VIN75_25155"
     CDS             3442..3924
                     /locus_tag="VIN75_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY8977526.1"
                     /translation="MKRFHVHVHVEDLAASIAFYSKLFAAAPARVQSDYAKWMLDDPR
                     INFAISSRGGQPGVDHLGLQADSEEELVDLKARAQAAGMTLLDEGETTCCYARSDKYW
                     LTDPDGVAWEQFHTLSTIPTFSETTKSGEPPQAACCAASAPRGKPVAIPVAAAKSSCC
                     "
     gene            3932..4618
                     /locus_tag="VIN75_25160"
     CDS             3932..4618
                     /locus_tag="VIN75_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY8977527.1"
                     /translation="MLRRKLLAEGLGTALLLAVVIGSGVMGQRLAGGNVAIALLANTF
                     ATVAGLVVLIELFGPVSGAHFNPAVSLVMWARGAPPRSALWPYIASQLAGAAVGAWLA
                     HAMFGLDIAQVSHRARDGGGQWIAEGVATFGLVLVVLRAPASRVAALVAAWIGAAYWF
                     TASTSFANPAAVFGRMLSDTFAGIAPASAPAFVIAELAGAALAVLVQRMLSPPAPVSV
                     PSPTAPTAHG"
     gene            4611..5039
                     /gene="arsC"
                     /locus_tag="VIN75_25165"
     CDS             4611..5039
                     /gene="arsC"
                     /locus_tag="VIN75_25165"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042278.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY8977528.1"
                     /translation="MVDVTIYHNPACGTSRNVLALIRHAGIEPVVIEYLKTPPSKERL
                     RELVAATGEPVRALLREKGTPYAELGLGDTKWTDDELLDFIVQHPILMNRPLVATPLG
                     TKLCRPSSESVLELLPVGPLAPFTKEDGEVVVDDGRRRGR"
     gene            complement(5103..5660)
                     /locus_tag="VIN75_25170"
     CDS             complement(5103..5660)
                     /locus_tag="VIN75_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8977529.1"
                     /translation="MLTAEQLVAAQKANLETLFELTNKAFEGVEKLVELNLQVAKASI
                     GEAQSTATAALNAKDAQELLALQAGLLQPAAEKAAAYGRHLYDIAATTNAEVTKIAEA
                     QVASAQANFNGAVDTAIKNAPAGTESAAALVKSAIAAATNAMESVQKATKQAADVAEA
                     NFQTLTNTAVKATSAPAKAAKKTTA"
     gene            5848..6801
                     /locus_tag="VIN75_25175"
     CDS             5848..6801
                     /locus_tag="VIN75_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY8977530.1"
                     /translation="MIAFHFDSHILPLPAGHRFPIGKYGLLRQRVAADPRGIALRVGG
                     PASEGELALAHEPGYIADVLTGTLAAARQREIGFDWTPALAARAVWSVGATIAAARVA
                     LSDGADGGVAASLGGGTHHANAAGGSGYCVFNDVAVAARLMQAEWHRRHRALLQVAVI
                     DLDVHQGNGTASIFADDDSVFTFSMHGERNFPFRKSPGDLDVGLPDGCDDAAYLAALR
                     PALDEVWRRMAGRPPGLVFYVAGADPHEGDRLGRLKLTTEGLAARDELVLAQLVERRI
                     PAVVLMAGGYGHVVDTTVDVHLQTLRIAERAQRAWGHVNTV"
     gene            6843..7325
                     /locus_tag="VIN75_25180"
     CDS             6843..7325
                     /locus_tag="VIN75_25180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8977531.1"
                     /translation="MNAPACPPTRRGPEPRAAYAAWRPVTTRWMDNDVYGHVNNVVYY
                     SFFDTAVNGLLMDAGVLDIHGGEVIGLVVETQCNYFAPIAFPQPVAAGVRVAQLGRSS
                     VRYEIGLYAGDALESAAAGHFVHVYVDRATRRPVPLPPSLKAVLQSWTVRPDSRTEQR
                     "
     gene            7322..8026
                     /locus_tag="VIN75_25185"
     CDS             7322..8026
                     /locus_tag="VIN75_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY8977532.1"
                     /translation="MSELPPNERQAAVDGAITSRRSVRAFLPTPVPEATIREILDVAA
                     RAPSGTNTQPWKVHVLTGAARRRLSDAILAAYNDPAERAQHSEEYDYYPREWVSPYID
                     RRRKIGWDLYGLLEIGKTDKDKMHAQHGRNYVFFDAPVGFIFTIDRILAQGSWLDYGM
                     FLQNIMVAARGRGLDTCPQAAFTQFHRVIKRELGLGDHEMVVCGMALGFADPEAVANR
                     LTTARAAVDEFTTFHT"
     gene            8023..9003
                     /locus_tag="VIN75_25190"
     CDS             8023..9003
                     /locus_tag="VIN75_25190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY8977533.1"
                     /translation="MTRLTVFIARRVFPDIVDRLRPHFEVRTHDGDAPLAQPALIAAL
                     QGCAGVFITGSEKIDAVLLDACPQLRAVCTMAVGYNNIDVPACTARGVVVTNAPGVLT
                     ETTADFGFALMMATARRISESERFLREGEWKHWGVDMFAGGDVHGATLGILGMGRIGQ
                     AIARRGHGGFDMKVAYHNRSHLAPDVEASLGATWMTLPDLLRTADHLVIVTPYTPETH
                     HLVGAAQLAQMKPTATLTNIARGGVVDDAALAEALAAGTIAAAGLDVFEGEPVVTPAL
                     LALRNVVLTPHIASASVPTRRAMANLAVDNLLAALGVGPDAGRPKTALNL"
     gene            complement(9255..>10397)
                     /locus_tag="VIN75_25195"
     CDS             complement(9255..>10397)
                     /locus_tag="VIN75_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY8977534.1"
                     /translation="VQHVAKASTTGHGTNIIAGLGVSMKSTAWPVLLVCAAIFVSYNL
                     AGLYGIAVAATSMLSMAGIVVALDAYGPITDNAGGIAEMAELPDSVRDITDPLDAVGN
                     TTKAVTKGYAIGSAGLAALVLFADYTHGLEGRGYHATFDLSDFRVIIGLFIGGMIPYL
                     FGAMAMEAVGRAAGSVVEEVRRQFRDIKGIMDGSGKPEYGKAVDMLTTAAIKEMVVPS
                     LLPVVVPVIVGLALGPAALGGLLMGTIVTGLFVAISMCTGGGAWDNAKKYIEDGNFGG
                     KGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLLPIASATSATSEPA
                     AAVAPAASDAASAIKPTPIAAPLPSMDGVTASTPSASMPASAASGQ"
BASE COUNT         1497 a         3677 c         3712 g         1511 t
ORIGIN      
        1 gcccaccgcc ttggccacgg ccagtccgcc gtcgcgcagg tcccagtcgt agacggcgcc
       61 cgaggtcacg aagcggtagc cgatgcagtt gtggcgatcg aggtcgtcga gccggcgcgg
      121 catgccgcgg tcggcgaggt aggccggcga ggccaccatg atcgagtgga acggcggcgt
      181 caggcgcacg gccaccatgt cctgcgccac cgtgtggccc agccggatgc cggcgtcgta
      241 gccctgggcg acgatgtcgg ccaggccgtc gtcggcggtg acttccaccg tgagcccggg
      301 atgccgccgc gccagctccg ccagcacggg cgtcaccgcg atgggcagcg ccgtgcgcgg
      361 cgagttcagg cgcagcgtgc cgctcacgcg gccctggtcg gcgcccaggc gctcggccgc
      421 ctcctcgatc tcggccagtg ccggaccgat gctcgcgacg aacgcggcgc cggcctcggt
      481 caggcgcacg ctgcgcgtcg tgcgcgcgaa cagcggctgc cccaggcccg cttccaccag
      541 ctgcaccgcg tgactgaccg cggacgggct caggcccagg tcgttggccg ccgccgcgaa
      601 gccgccgcgc cggtgcacgg cgagcacgat cggcaggtgc aggagcaggg ctcggtccat
      661 tcatgaatga tatcgaacaa ggcatgcgat caatgcagct ttatcgatct atcgacctgg
      721 aggacattga ggccatgcgc tgcaccccgc ggcgtcccaa ccgaaaggcc tcgccatgaa
      781 caccaccgcc cagacctggt tcatcaccgg cagctcctcc ggcttcggcc gcgcgttcgc
      841 cgagcacgcg atcgcgcagg gccacaacgt cgtcgccacg gcgcgccgcg tcgatgcact
      901 ggccgacctc gtcgcgcagg cgcccgggcg cgtgctggcg ctggcgctgg acgtcgacgt
      961 cgcgcgggac gcggaggccg cggtcggcgc ggcggtcgcg cgcttcgggc gcatcgacgt
     1021 gctggtcaac aacgccggct acggcgtcgt cggcgcgctg gaggagacgc ccgacgccga
     1081 gctgcgcgcg ctgttcgaca ccaacttctt cggcgcgctc gcgctcacgc gcgccgcgct
     1141 gccggtgctg cgcgagcagc ggggcggagc catcgtcaac atgtcgagcc tgggcggaca
     1201 gctgtcgttc gcgggcttca gcgcctactc ggcgaccaag ttcgcgctgg aaggcgcgtc
     1261 cgaggcgctg gcgcaggaag tcgcgccgtt cggcatcaag gtgctgatcg tcgaaccggg
     1321 ccagttccgc acccagctcg ccggcgcggg catgcgccac atgcccgagc tcgaggcgta
     1381 tgccgcgacg gtgggcggca cgcgcacgtt cgcgcacgac atgcaccaga cgcaggccgg
     1441 cgacccgcgc aaggccgccg tcgcgattga gcgtgcgctc gccgcgcccg tcacgccgct
     1501 gcggctgcag ctgggcggcg actcggtcga cgccgtgcgg gcgcatgcgc aggcgctgct
     1561 ggccgacctc caggcgtggg aggccgtggc ccgcggcacc gatttcgacg cgcgctgaac
     1621 gtcagcgcgc cgtcacgccg gccagcgtca gcagcagccc ggcgagcgcg cagcccgcga
     1681 tcaccgggat cacgccggcc cgcaaccgga acagcgccac cgcggcggcg atgccgatcg
     1741 cgagcgccgc cgcgtcgaag cgtccgcccg ggccgtgcgg ccacagcacg tgcagcgcga
     1801 agaaggccgc gaggttgacg atgacgccga ccaccgccgc cgtcacgccg gtcagcggtg
     1861 cggtgaactt caggttgccg tgcgtcgatt cgatgaccgg cccgcccacc aggatgaaga
     1921 agaacgaggg caggaaggtg aagaacgtga ccacggtcgc cgccgccgcg ccggccagcg
     1981 cgagtgcgcc cggcccgaac acggcctgcg tccagccgcc gacgtagccg acgaaggcga
     2041 cgaccatgat cagcgggccc ggcgtggtct cgccgagcgc gagcccgtcc atcatctgcg
     2101 ccgcggtgag ccagtggtag tggtcgacgc cgccctgcat cacgtagggc aggatggcgt
     2161 aggcgccgcc gaaacccagc cacgcggtct tcgtgaagaa ccaggccatc tgcgcgagcg
     2221 tgccgttcca gccggtcgcg gcggccagcg ccgcgagcgc gccgccccac agcgccgcga
     2281 acgccgcgac gatgcgcagc gcgcgcggcc aggcgtgacg cgcgtgcgcg ggcacggggg
     2341 tcgcgtcgtc gatcagggcg ggggcgtggc tgccggacgc gccgggccgc gccgggccgg
     2401 gtcggaactt gtccggcgcc agccacccgc cgagcgcgcc ggcgagcgcg gccagcagca
     2461 cgatcagcgg gaacggcaga tgcagcacga agatcgccac gaaggacgcg acggcgatgc
     2521 cccacagcca cgcgttggtc aaggtgcgcg agcccacgcg ccacgcggcg tgcagcacga
     2581 gcgcggtcac ggccggcttg atcccgtgga acaggcccgc gatcgtcggc acctggccga
     2641 acgccatgta gacccacgac agcccgatca ggatgaacag cgacggcagc acgaacagcc
     2701 cgcccgccac gatcccgccg atcgtgcggt gcatcagcca gccgacgtag gtcgccagct
     2761 gctgcgcctc cgggcctggc agcagcatgc agtagttcag cgcgtgcagg aagcgctgct
     2821 cagagatcca gcgccggcgc tcgaccagct cctcgtgcat cacggcgatc tgccccgcgg
     2881 ggccgccgaa gctgatgaag cccagcttga gccagaaggc gaaggcctcg cggcgaggcg
     2941 gcatggcggc gggcgtggat ggggtaccgg cgatgccggt gtcgtgcagc gacatggggc
     3001 gggatggtag cgagccgcga tgacgagact tgcatgtcgt cgtgtatcgc gataatatcg
     3061 aaaaaactcg atatgacgat ttcagccatc tccaaaccgg atgtgctcgc ggccctggcc
     3121 gtgctggccc accccgtccg cctcgacgtc ttccgcgtcc tggtgcaagc cgggccggtc
     3181 ggcctcacgc cgaccgcgct ggcggcgctc gtccagccgg agctcaggca gagcacgttg
     3241 gccacgcacc tgaaggaact ggtcaccgcc ggcctggtcg cgaacgcgcg ggccgggcgc
     3301 aacgtcgtct atcgcgccga ctacgagcgc atgggcggcg tcatcgccta tctcaccgag
     3361 aactgctgcg ccggcgcacc ctgcgccgtc gaacccacct gccagtccgc ctgcggctgc
     3421 tgacccacca aggagtccgt catgaagcgt ttccacgtcc atgtgcatgt cgaagacctt
     3481 gccgcgagca tcgcgttcta ctcgaagctg ttcgccgccg cgcctgcgcg cgtccagagc
     3541 gactacgcca agtggatgct cgatgaccca cgcatcaact tcgcgatctc cagccgcggc
     3601 ggccagcccg gcgtcgacca cctcggcctg caggccgaca gcgaggagga gctcgtcgac
     3661 ctcaaggcac gcgcgcaggc cgccggcatg acgctgctcg acgaaggcga gacgacgtgc
     3721 tgctacgcgc gcagcgacaa gtactggctg accgacccgg acggggtcgc gtgggagcag
     3781 ttccacacgc tgtcgacgat ccccaccttc agcgaaacca cgaagtccgg cgaaccgccg
     3841 caggccgcgt gctgtgccgc gtcggcgccg cgcggcaagc cggtcgccat tcccgtcgcg
     3901 gcggcgaagt cgtcctgttg ctgaggcgcc ggtgctgcgt cgcaaattgc ttgccgaggg
     3961 gctcgggacc gcgttgcttc tggcggtggt catcggctcg ggcgtgatgg ggcagcggct
     4021 ggccggcggc aacgtcgcca tcgcgctgct ggccaacacg ttcgctacgg tggccgggct
     4081 ggtcgtgctc atcgagctgt tcgggccggt gtccggcgcg catttcaatc cggcggtgtc
     4141 gctggtgatg tgggcgcgcg gagcgccgcc gcgatcggcg ctgtggccct acatcgcgtc
     4201 gcagttggct ggcgccgcgg tcggggcgtg gctggcgcat gcgatgttcg gcctcgacat
     4261 cgcgcaggtg tcgcaccgcg cgagagacgg cggggggcag tggatcgccg aaggcgtcgc
     4321 cacgttcggg ctcgtgctcg tggtgctgcg tgcgccggcg tcgcgcgtcg ccgcgctggt
     4381 ggcggcctgg atcggcgccg cctactggtt cacggcctcg acgtcgttcg ccaatcccgc
     4441 ggccgtgttc gggcgaatgc tcagcgatac atttgccggc atcgcgcccg ccagcgcgcc
     4501 ggcgttcgtg atcgcggagc tcgcaggcgc cgcgctggcc gtcctcgtcc agcgcatgct
     4561 cagcccgccc gcgccggtgt ctgttccatc cccgactgcc cccacggccc atggttgacg
     4621 tcacgatcta tcacaacccc gcctgcggca cctcgcgcaa cgtgctcgcg ctgatccgcc
     4681 acgccgggat cgagcccgtc gtcatcgagt acctgaagac gccgccgagc aaggagcggt
     4741 tgcgcgagct ggttgccgcc accggcgagc cggtgcgggc gctgctgcgc gagaagggca
     4801 cgccttacgc ggagctgggc ctgggcgaca cgaagtggac ggatgacgag ttgctcgact
     4861 tcatcgtgca gcacccgatc ctcatgaacc gtccgctcgt ggcgacgccg ctgggcacca
     4921 agctgtgccg gccgtcttcg gagtccgtgc tggagctgct gccggtgggg cctcttgcgc
     4981 cgttcacgaa ggaggacggc gaggtcgtgg tcgacgacgg ccggcggcgc ggccgctgag
     5041 cgcgcggcgc cgaaacgaaa aacccggccg gagccgggtt tcgtcttcat gcgacctgca
     5101 ggtcaggcgg tcgtcttctt ggcagccttg gccggcgccg aggtggcctt gacggcggtg
     5161 ttcgtcagcg tctggaagtt ggcttccgcg acgtcggccg cctgcttggt ggccttctgc
     5221 acgctctcca tcgcgttggt ggcggcggcg atggccgact tcaccagggc ggcggcggac
     5281 tcggtgccgg ccggcgcgtt tttgatggcg gtgtcgacgg cgccgttgaa gttggcctgg
     5341 gcggaggcca cttgcgcttc ggcgatcttg gtcacttcgg cgttggtggt ggcggcgatg
     5401 tcgtacaggt ggcgaccgta ggcggcggcc ttctcggcgg cgggctgcag caggccggct
     5461 tgcagggcca gcagctcctg ggcatccttg gcgttcaggg cggccgtggc ggtgctctgg
     5521 gcttcgccga tcgaggcctt ggcgacctgc aggttcagtt cgacgagctt ttcgacgcct
     5581 tcgaacgcct tgttggtcag ctcgaacagg gtttcgaggt tggccttctg ggcggcgacg
     5641 agttgttcag cggtcagcat gaggtgaatc tcctgggttt cggtggaaac tgggttgcat
     5701 cagggttgca tcgcgccagc aagttgattg ctgcagtgca acatgaatcc aagtataggg
     5761 cttggtcggc cgtttgcaag caaatctgct gcagtgcagc acgttctagg ggcatccctc
     5821 caggggtcat actttttaca ccggaccgtg atcgctttcc acttcgattc gcacatcctg
     5881 cctttgcctg cgggtcatcg gttcccgatc ggcaagtacg gcctcctgcg ccagcgggtg
     5941 gcggccgacc cgcgcggcat cgcgctgcgc gtcggcggcc ccgccagcga gggcgagctg
     6001 gcgctggccc acgagcccgg ctacatcgcc gacgtcctga cgggcacgtt ggccgctgcc
     6061 cggcagcgcg agatcggctt cgactggacg cccgcgctgg ccgcgcgcgc cgtctggtcg
     6121 gtcggggcga ccatcgcggc cgcgcgggtg gcgctgtcgg acggcgcgga cggcggcgtc
     6181 gccgcgagcc tgggcggcgg cacgcaccac gcgaacgcgg cgggcggttc gggctactgc
     6241 gtgttcaacg acgtggccgt cgccgcgcgt ctgatgcagg ccgagtggca ccgccgccat
     6301 cgcgcgctgc tgcaggtggc cgtgatcgac ctggacgtgc accagggcaa cggcaccgcg
     6361 tcgatcttcg cggacgacga cagcgtgttc acgttctcga tgcacggcga gcgcaacttc
     6421 ccgttccgca agtcgcccgg cgacctcgac gtcggcctgc ccgacggctg cgacgacgcc
     6481 gcctacctgg ccgcgctgcg gccggcgctg gacgaggtct ggcgccgcat ggccgggcgt
     6541 ccgcccggtc tcgtgttcta cgtcgccggc gccgacccgc acgagggcga ccgcctcggc
     6601 cgtctcaagc tgacgaccga aggcctcgcg gcgcgcgacg agctcgtgct ggcccagctc
     6661 gtcgaacgac gcatcccggc ggtggtgctg atggccggcg gctacggcca cgtcgtcgac
     6721 accacggtgg acgtgcacct gcagacgctg cgcatcgccg agcgcgcgca gcgggcttgg
     6781 gggcacgtca acaccgtgtg aggcgagctc gcacacaatg ggtgaaacgt cccgaccact
     6841 cgatgaacgc tcccgcctgc ccaccgaccc gccgcggccc cgagccgcgc gccgcctatg
     6901 ccgcgtggcg tcccgtcacc acgcgctgga tggacaacga cgtctacggc cacgtcaaca
     6961 acgtcgtcta ctacagcttc ttcgacaccg ccgtgaacgg cctgctgatg gacgcgggcg
     7021 tgctcgacat ccacggcggc gaggtcatcg gcctggtggt cgagacgcag tgcaactact
     7081 tcgcgccgat cgcattcccg cagccggtgg ccgcgggcgt gcgcgtggcg cagctcgggc
     7141 gctcgagcgt gcgctacgag atcggcctgt acgccggcga cgccctcgag agcgcggccg
     7201 ccggccattt cgtccacgtc tacgtcgacc gcgccacgcg caggccggtg ccgctgccgc
     7261 cgtcgttgaa ggcggtgctg cagtcgtgga cggtccgccc ggactctcga acggaacaac
     7321 gatgagcgaa ctgcctccca acgaacgcca ggcggccgtc gacggcgcca tcacgtcgcg
     7381 ccgctcggtg cgcgccttcc tgcccacgcc ggtgcccgag gccacgatcc gcgagatcct
     7441 cgacgtcgcc gcgcgcgcgc cctcgggcac gaacacgcag ccgtggaagg tgcatgtgct
     7501 gaccggcgcg gcgcgccggc gcctgagcga cgccatcctc gcggcgtaca acgatcccgc
     7561 cgagcgcgcg cagcactccg aggagtacga ctactacccg cgcgaatggg tgtcgcccta
     7621 catcgaccgc cgccgcaaga tcggctggga cctgtacggc ctgctggaga tcggcaagac
     7681 ggacaaggac aagatgcacg cccagcacgg ccgcaactac gtgttcttcg atgcgccggt
     7741 gggcttcatc ttcacgatcg accgcatcct ggcgcagggc agctggctgg actacggcat
     7801 gttcctgcag aacatcatgg tcgccgcgcg cggccgcggc ctcgatacct gcccgcaagc
     7861 cgcgttcacg cagttccacc gcgtgatcaa gcgcgagctg gggctgggcg accacgagat
     7921 ggtggtgtgc ggcatggccc tcggcttcgc cgaccccgag gccgtcgcga accgcctgac
     7981 cacggcgcgc gccgccgtcg acgaattcac caccttccac acatgacccg cctcaccgtc
     8041 ttcatcgcgc gccgggtctt tcccgacatc gtcgaccgcc tgcggccgca cttcgaggtg
     8101 cgcacgcacg acggcgatgc gccgctcgcg cagcccgcgc tcatcgccgc gttgcagggc
     8161 tgcgccggcg tcttcatcac cggcagcgag aagatcgacg ccgtgctgct ggacgcctgc
     8221 ccgcagctca gggccgtgtg cacgatggcc gtgggctaca acaacatcga cgtgccggcg
     8281 tgtaccgcgc gcggcgtggt cgtcaccaac gcgccgggcg tgctcaccga gacgacggcc
     8341 gacttcggct tcgcgctgat gatggccacc gcgcgccgca tctccgagag cgaacgtttc
     8401 ctgcgcgagg gcgagtggaa gcactggggc gtggacatgt tcgccggcgg cgacgtccat
     8461 ggcgccacgt tgggcatcct cggcatgggc cgcatcggcc aggcgatcgc caggcgcggc
     8521 cacggcggct tcgacatgaa ggtggcatac cacaaccgca gccacctcgc gcccgatgtc
     8581 gaagcctcgc tgggcgcgac gtggatgacg ctgcccgacc tgctgcgcac ggccgaccac
     8641 ctcgtcatcg tcacgcccta cacgcccgag acccaccacc tggtcggcgc cgcgcagctc
     8701 gcgcagatga agccgaccgc gacgctcacc aacatcgcgc gcggcggcgt ggtcgacgac
     8761 gcggcgctgg ccgaggccct ggccgccggc acgatcgcgg ccgcgggcct ggacgtgttc
     8821 gaaggcgagc ccgtcgtgac cccggcgctg ctcgcgctgc gcaacgtcgt gctcacgccg
     8881 cacatcgcca gcgccagcgt gccgacgcgg cgcgcgatgg ccaacctcgc cgtggacaac
     8941 ctgctggcgg cgctgggcgt cgggccggat gcgggcaggc cgaagacggc gttgaacctg
     9001 tagggcgggt ctcggcccgg aaggggtctg gcctttcctc gtttcgcgcc acgttgccgc
     9061 cgaagcgacg acgcttgtcg cgaaacgagg acaggcctga ccccgatcgt gagcaggggg
     9121 tcggggtcag gcctgtcctc gaatcgcatt cagcgttgcg gccatcgagg agaaccgcgc
     9181 gcgattcgag gagaggccag accccttcgg ccctttgcct ggcgcgcggg tgacgcgcgc
     9241 gggaggcggg cggcttactg gccggaggcg gccgacgccg gcatggacgc cgacggcgtg
     9301 gaggccgtca cgccgtccat cgacggcagc ggcgccgcga tcggggtggg cttgatcgcc
     9361 gacgcggcgt cggacgccgc gggagcgacg gccgcggccg gttcggaggt ggccgacgtc
     9421 gccgaggcga tcggcagcag cggcacgatc agcagcgcga cgatgttgat gatcttgatc
     9481 agcgggttga cggccgggcc ggccgtgtcc ttgtacgggt cgccgacggt gtcgccggtg
     9541 accgcggcct tgtgggcctc gctgcccttg ccgccgaagt tgccgtcctc gatgtacttc
     9601 ttggcgttgt cccacgcgcc gccgccggtg cacatcgaga tggccacgaa caggcccgtc
     9661 acgatggtgc ccatcagcag gccgccgagc gcggccgggc cgagggccag gccgacgatc
     9721 accggcacca cgaccggcag cagcgacggc accaccatct ccttgatggc ggcggtggtc
     9781 agcatgtcga cggccttgcc gtattcgggc ttgccgctgc cgtccatgat gcccttgatg
     9841 tcgcggaact ggcgacgcac ttcctcgacc accgagcccg ccgcgcggcc gacggcctcc
     9901 atcgccatgg cgccgaacag gtaggggatc atgccgccga tgaacaggcc gatgatcacg
     9961 cggaaatccg ataggtcgaa ggtggcgtgg tagccgcggc cctcgaggcc gtgggtgtag
    10021 tcggcgaaca gcaccagcgc ggccaggccg gccgagccga tcgcgtagcc cttggtgacg
    10081 gccttggtcg tgttgccgac ggcgtccagc gggtcggtga tgtcgcgcac gctgtcgggc
    10141 agttccgcca tctcggcgat gccgccggcg ttgtcggtga tcgggccgta ggcgtcgagc
    10201 gcgacgacga tgccggccat gctcagcatc gacgtggccg ccaccgcgat gccgtagagg
    10261 cccgcgaggt tgtaggacac gaagatggcg gcgcacacca gcagcaccgg ccaggcggtg
    10321 gacttcatcg acacgcccag gccggcgatg atgttggtgc cgtggccggt ggtcgacgcc
    10381 ttggccacgt gctgcac
//
LOCUS       DATCRO010000926         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_629296, whole genome shotgun sequence.
ACCESSION   DATCRO010000926 DATCRO010000000
VERSION     DATCRO010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629296"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VIN75_25200"
     CDS             <1..262
                     /locus_tag="VIN75_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977535.1"
                     /translation="LRAMWSDAGLVDVETSEVTVKRRFADFETWWATARKGPVFAPRL
                     SGTSGGNSEVLKARLRERLDAAADGSITCSGRATAVRGRKPG"
     gene            complement(275..1663)
                     /locus_tag="VIN75_25205"
     CDS             complement(275..1663)
                     /locus_tag="VIN75_25205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8977536.1"
                     /translation="MTPARSARPILYRQLTLRLSIPLLVIVAVVGATGLFSVGAETSA
                     VFDRWLLDAAVSLADQVRSADGTGANAVDLPEAARTMLAYDEIDHTWFSVEDRGRVLV
                     GSAGIPASGVNAATYPDGRAYDAVYEGAPVRVAAVKAPCAGCEHVVVLVAETTLKRQR
                     SNRVVEWLLIPLALMLVVTCSAIYIAVRRTTRPLESLAEHWNRQAHASLEAIPEGDLP
                     SELSPFATALNDLLSRIRGMLVRERMFAAAAAHQLRTPLTALRLGMARARNSPDLESA
                     RAVLDELSQVTEHTGRLVQQLMLLGRLDPDRRGDLDRAPADLRDIARDVCGLFAEVAL
                     DQHVDLELQVPATPVTVTAQAELLAEALANLIDNAMKAAGHKGQVLVAVIESPPGLRV
                     CDSGPGIKPSERRVVFERYARGSRPRWEGTGLGLAIVHDVVAMHGAKVTVSDGELGGA
                     CFAIEFPWPARA"
     gene            complement(1660..2403)
                     /locus_tag="VIN75_25210"
     CDS             complement(1660..2403)
                     /locus_tag="VIN75_25210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8977537.1"
                     /translation="MRLLLIEDDIHIARELLLRWRDGERLALHAPTLADADAALAQGS
                     FDIVLLDLGLPDGDGMDWLTAYRQRDPQGAVLVMTARDRVADRVKGLRLGADDYLVKP
                     FAPEELDARIDVLVRRANVARTHAIRFGRLDLIQESSEVLLDGKRLDLSPREFELLFI
                     LMRAAPRLVPKRALVDALSERNLELNDAAAELYVSRLRKKLDGTGTGIRTLRGVGYQL
                     AIVAPGPSGVPAAPPVRTADNGEGSEGGA"
     gene            2605..3603
                     /locus_tag="VIN75_25215"
     CDS             2605..3603
                     /locus_tag="VIN75_25215"
                     /inference="COORDINATES: protein motif:HMM:NF016293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEY8977538.1"
                     /translation="MLLTTLDARGVRAGAPLTWPGARGMFDNAFDLNLERRLKYAGRL
                     ALTWRARARLLRELAGLTLGEALFRAQPRAFYPVMSHLIDRRMGVQARVRATLTSLRA
                     VCESVGDEANVRSLLTHGITLLELADHTRIVLSLNDVSFHEGLWQLGLVGADGLRLYS
                     IGFGFTDARTLLMGNVQGPSLKDEGLDRIREATHAAHGMRPPHLLVHALRLLAAHWGA
                     TRLLGVDPENHVKGRWNLRDSRLRFDYRAFWAEHEAVRDEGGNWSLPLETALRPLEEV
                     PTKRRAMYRRRYAMLEALQQAVDTLADARHALPPVPVDTPELAAANAPTLEPVLVA"
     gene            complement(3762..4985)
                     /locus_tag="VIN75_25220"
     CDS             complement(3762..4985)
                     /locus_tag="VIN75_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY8977539.1"
                     /translation="MTIDPGIKLLIVDDLPENLMALEASIRDEAWSVHQASSGEEALA
                     LLLEHEFALAILDVQMPGMNGFELAELIRGMARTRHLPIVFVSAAGKELDYAFKGYEN
                     GAVDFLYKPLDAYAVKSKVQVFVDLYRQRTELRRQLAALEESRRRQQALVAELQATQI
                     ELEGALRMRDEFMSMVVHELRTPLSVLAMEVRVRQHQVAAGNTTFFSPENLSRMFERD
                     QRQVRSLTRLIDDMLDVSQIQHGKLSIRRGACDLAAMMRRVVAEVQGQRSDVPITLKA
                     DGVDVVGEWDEFRLEQVVVNLLTNALRYGAGKPIRVELDQLPGAATIRVSDQGVGIAP
                     DDKARVFEQFVRVGDRSRIPGLGLGLYITRQLVEAHGGTISVESTLGEGSTFTVALPT
                     ASAEAVPARQAAKAA"
     gene            complement(4982..>5110)
                     /locus_tag="VIN75_25225"
     CDS             complement(4982..>5110)
                     /locus_tag="VIN75_25225"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8977540.1"
                     /translation="VQDPATAEVPTMPGAAIRRMAPDFILPLAEIRSLLQRLGEHR"
BASE COUNT          722 a         1820 c         1819 g          749 t
ORIGIN      
        1 gctgcgcgcg atgtggtccg atgccggcct ggtcgatgtc gagaccagcg aggtcacggt
       61 caagcgccgc ttcgccgact tcgagacctg gtgggccacc gcgcgcaagg gcccggtgtt
      121 cgcgccgcgg ctgtcgggga cgtccggcgg caactcggaa gtgctcaagg cgcgcctgcg
      181 cgaacgcctg gacgcggcgg ccgacggcag catcacctgc agcggccggg cgaccgcggt
      241 gcgcggccgc aagccgggct gacgggcccg cgattcaggc cctggccggc cacgggaact
      301 cgatcgcgaa gcaggcgccc ccgagctcgc cgtcgctgac ggtcaccttg gcgccgtgca
      361 tcgccacgac gtcgtgcacg atcgccaggc ccaggccggt gccctcccag cgcggacgcg
      421 agccgcgcgc gtagcgctcg aagaccacgc ggcgctcgga cggcttgatg ccggggccgc
      481 tgtcgcacac gcgcagcccg ggcggcgact cgatcaccgc caccagcacc tggcccttgt
      541 ggcccgcggc cttcatcgcg ttgtcgatca ggttggcgag cgcctcggcc agcagttccg
      601 cctgcgcggt caccgtgacg ggcgtggccg gcacctgcag ttcgaggtcg acgtgctggt
      661 cgagcgccac ctccgcgaac agcccgcaga cgtcgcgcgc gatgtcgcgc aggtcggccg
      721 gcgcgcggtc gaggtcgccg cggcgatcgg ggtcgagccg gcccagaagc atcagttgct
      781 gcaccaggcg gccggtgtgc tcggtcacct gcgacagctc gtccagcacg gcgcgcgcgc
      841 tctcgaggtc ggggctgttg cgtgcccgtg ccatgcccag gcgcagcgcg gtcagcggcg
      901 tgcgcagctg gtgcgcggcg gcggcggcga acatgcgctc gcgcacgagc atgccgcgga
      961 tgcgcgagag caggtcgttc agcgcggtgg cgaatggcga cagctccgac ggcaggtcgc
     1021 cctcggggat cgcctccagc gacgcgtgcg cctgccggtt ccagtgctcg gccagcgatt
     1081 ccagcggacg cgtcgtgcgc cgcaccgcga tgtagatggc cgagcacgtc accaccagca
     1141 tcagcgcgag cgggatcagc agccattcga cgacgcggtt ggaccgctgg cgcttgagcg
     1201 tcgtctcggc caccaggacc acgacgtgct cgcagccggc gcatggcgcc ttgacggcgg
     1261 ccacgcgcac cggcgcgccc tcgtagacgg cgtcgtaggc gcggccgtcg gggtaggtcg
     1321 cggcgttgac gccgctggcg gggatgcccg cgctgcccac cagcacgcgg ccgcgatcct
     1381 cgacgctgaa ccaggtgtgg tcgatctcgt cgtaggccag catcgtgcgc gcggcctcgg
     1441 gcaggtcgac ggcgttggcg ccggtgccgt cggcgctgcg cacctggtcg gccagcgaca
     1501 cggccgcgtc cagcagccag cggtcgaaca cggcggaggt ctcggcgccg acgctgaaca
     1561 ggcccgtcgc gccgaccacc gccacgatca ccagcagcgg gatgctcagg cgcagcgtca
     1621 gctgccggta caggatcggc cgcgccgacc gcgctggcgt cacgcgccgc cctcgctgcc
     1681 ctcaccgttg tcggcagtgc gcacgggtgg cgccgcgggc actccggacg gccccggcgc
     1741 cacgatcgcc agctggtagc cgacgccgcg cagggtacgg atacccgtac cggttccatc
     1801 gagcttcttg cgcagccgcg acacgtagag ctccgcggcg gcgtcgttca gctcgaggtt
     1861 gcgctcggac agcgcgtcca ccaacgcccg cttgggcacc agccgcggcg ccgcgcgcat
     1921 caggatgaac agcagctcga actcgcgcgg cgacaggtcc agccgcttgc catccagcag
     1981 cacctcggag ctctcctgga tcaggtcgag gcggccgaag cggatggcgt gcgtgcgcgc
     2041 gacattggcg cgccgcacca gcacgtcgat acgggcgtcg agctcctcgg gcgcgaacgg
     2101 cttgacgagg tagtcgtcgg cgcccaggcg caggcccttg acgcggtcgg cgacgcgatc
     2161 gcgcgcggtc atcaccagca cggcgccctg cgggtcgcgc tggcgatagg cggtgagcca
     2221 gtccatgccg tcgccatccg gcagtccgag atccagcagc acgatgtcga agctgccctg
     2281 cgccagcgcg gcgtccgcgt cggccagcgt cggcgcgtgc agcgcgaggc gctcgccatc
     2341 gcgccaccgc aggaggagct cgcgggcgat gtggatgtcg tcttcgatca ggagcagtct
     2401 catggctgtg ggattgtcgc ttgagtgccc cgggtgcagg cgtcgccggg ctgtcgtgag
     2461 tcctgaatgt gcggaatcga cattctggag atacaaaccg gcaacatacc cgatcgtgcc
     2521 aatgggtgat agcgattcga taggtggtct tctcaaactc gccgctcgaa gagcgggcat
     2581 ccgctcacca gctgacggat cccaatgctt ctgaccaccc tggacgcccg cggcgtccgc
     2641 gcgggcgccc ccttgacttg gccgggcgcg cgcggcatgt tcgacaacgc gttcgacctg
     2701 aatctcgaac ggcgcctgaa atacgccggc cgcctcgcgc tcacgtggcg cgcccgcgcg
     2761 cgactgctgc gcgagctcgc cggcctgacg ctcggcgaag ccctgttccg cgcccagccg
     2821 cgtgccttct atccggtcat gagccacctc atcgaccgtc gcatgggcgt ccaggcccgc
     2881 gtgcgcgcca cgttgacaag cctgcgcgcg gtgtgcgagt cggtcggcga cgaggccaac
     2941 gtgcgcagcc tgctcacgca cggcatcacg ttgctggagc tggccgacca cacgcgcatc
     3001 gtgctgtcgc tcaacgacgt gagcttccac gagggcctgt ggcagctggg cctggtcggc
     3061 gccgacggcc tgcggctgta cagcatcggc ttcggtttca ccgacgcacg cacgcttctc
     3121 atgggcaacg tgcagggccc gtcgctgaag gacgagggcc tggaccgcat ccgcgaagcc
     3181 acccatgccg cgcacggcat gcggccgccg cacctgctgg tgcacgcgct gcgcttgctg
     3241 gcggcgcact ggggcgcgac gcgcctgctg ggcgtcgatc cggagaacca cgtcaagggc
     3301 cgctggaacc tgcgcgactc gcgcctgcgc ttcgactacc gtgcgttctg ggccgagcac
     3361 gaggccgtgc gcgacgaggg cggcaactgg tcgctgccgc tggagacggc gctgcgcccg
     3421 ctggaggagg tgcccaccaa gcgccgcgcg atgtaccgcc gccggtatgc gatgctcgaa
     3481 gcgctgcagc aggccgtcga cacgctggcc gatgcgcgcc atgcgctgcc gccggtgccg
     3541 gtggacacgc cggaactggc cgcggcgaat gcaccgacgc tggagccggt gctggtcgcc
     3601 tgagccgcca agccggttgc gatgaccgcc tgttcgtagg gcgggttagg cgcagccgta
     3661 acccgccgtt cgtcggcgca ctgcgacggg gcctccgtat tgaaagcgcc ggcggatcgg
     3721 cgtgcaaccg aacggcgggt tacggctgcg cctaacccgc cctacgcagc tttcgcggcc
     3781 tggcgcgccg gcaccgcctc cgcacttgcc gtcggcaagg ccacggtgaa ggtggaccct
     3841 tctcccagcg tgctctccac gctgatcgtg cccccgtggg cctccaccag ttgcctggtg
     3901 atgtacaggc ccaggcccag gccggggatg cgggagcggt cgcccacgcg tacgaactgc
     3961 tcgaacacgc gcgccttgtc gtcgggcgcg atgcccacgc cctggtcgct gacgcggatc
     4021 gtcgcggcgc cgggcagctg gtccagctcg acccggatcg gcttgcccgc gccgtagcgc
     4081 agcgcgttgg tcagcaggtt gacgacgacc tgctccagcc ggaactcgtc ccactcgccg
     4141 accacgtcga cgccgtcggc cttcagcgtg atcggcacgt cgctgcgctg gccctgcacc
     4201 tcggcgacca cgcgccgcat catcgccgcc aggtcgcacg cgccgcggcg gatcgacagc
     4261 ttgccgtgct ggatctgcga gacgtccagc atgtcgtcga tcaggcgagt gaggctgcgc
     4321 acctggcgct ggtcgcgctc gaacatcctg gacaggttct ccggcgagaa gaacgtggtg
     4381 ttgcccgcgg cgacctggtg ctggcgcacg cgcacctcca tcgccaggac gctcagcggc
     4441 gtgcgcagct cgtgcaccac catcgacatg aactcgtcgc gcatgcgcag cgccccttcc
     4501 agctcgatct gcgtggcctg cagctcggcg acgagcgcct gctggcggcg gcgcgactcc
     4561 tcgagcgcgg cgagctggcg gcgcagctcc gtgcgctggc gatagaggtc gacgaacacc
     4621 tgcaccttgc tcttgacggc gtaggcgtcc agcggcttgt agaggaagtc gacggcgccg
     4681 ttctcgtagc ccttgaacgc gtaatcgagt tccttgccgg cggcgctgac gaacacgatc
     4741 ggcaggtgcc tggtgcgcgc catgccgcgg atcagctcgg ccagctcgaa gccgttcatg
     4801 ccgggcatct gcacgtccag gatcgcgagg gcgaactcgt gttcgagcag cagcgccagc
     4861 gcctcctcgc cggaggacgc ctggtgcacg ctccaggcct cgtcgcggat ggaggcctcc
     4921 agggccatca ggttctcggg aagatcgtcg acgatcagga gcttgatgcc agggtcaatc
     4981 gtcatcggtg ttctccaagc ctttgcagca aggagcggat ctctgcgagt ggaaggatga
     5041 agtcgggcgc catgcggcgg atcgcggctc cgggcatcgt cgggacttcg gcggtggccg
     5101 gatcctggac
//
LOCUS       DATCRO010000927         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_776224, whole genome shotgun sequence.
ACCESSION   DATCRO010000927 DATCRO010000000
VERSION     DATCRO010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776224"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VIN75_25230"
     CDS             complement(<1..477)
                     /locus_tag="VIN75_25230"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8977541.1"
                     /translation="MTEAPSTADDPDAPRVLLETTDWSRTPLGQRETWPRALRGYVEM
                     VLDMPTPAIIFWGPEQTQIYNDGYALILGPRHPRGFGMPYREIWPDTSPLIYPWMRHV
                     LDNDGTWRVDREHIPVTRHGFDEEAYFTFTFSPLRDDDGRIMGILQPVFDVSETVLA"
     gene            793..>2233
                     /locus_tag="VIN75_25235"
     CDS             793..>2233
                     /locus_tag="VIN75_25235"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY8977542.1"
                     /translation="MTAAPFMRALPFAVALALAACGGGGGGTDTSNNGTSSPSSADTA
                     VSSGAISAFGSVFVNGHEFATGSARVMDDDTGDTAGRVSELEVGQVVDVEPSSSSTTA
                     APVAQWLHVHPLVRGYVDASDTTAGTITVMGQVVSLDSGTLFSDHRACVAATTNACTA
                     VTDASGLVATTGGTPGTYATVDGYLFSSTAGSAQIVATLVSVRDVPTSYPAAFKAEGV
                     VGATTVGGATAGVTIGGLAVDLDHATCRVDGKPVACATAFAAGEVVSVFSATAPALPA
                     TAFNATVAIERRHLPVQTAGQAVEFDGRVSASTPASGTMAATFVVRGVTVDTSQMPAG
                     TSLPAVGDMVRVAGTVSADGLSVVATSIKITHAARSVSYALSGDVTAVAAGTVSGTFN
                     VTVLGQTIVVSDQTRLSDRQTWQWMDDDPQSNPFNIATFQTYLAASASQHVAVKAAKD
                     SSGVLQALSFAIWPASTFSEVAGPVDATPA"
BASE COUNT          324 a          778 c          794 g          337 t
ORIGIN      
        1 ggccagcacc gtctccgaca cgtcgaacac cggctgcagg atgcccatga tccggccgtc
       61 gtcgtcgcgc agcgggctga acgtgaaggt gaagtaggcc tcctcgtcga agccgtggcg
      121 cgtgacgggg atgtgctcgc gatccacccg ccaggtgccg tcattgtcga gcacgtgccg
      181 catccacggg tagatcaggg gggaggtgtc cggccagatc tcccggtaag gcatgccgaa
      241 gccgcgggga tggcgcggac ccaggatcag ggcgtagccg tcgttgtaga tctgcgtctg
      301 ctccgggccc cagaagatga tggccggggt gggcatgtcc aacaccatct ccacgtagcc
      361 gcgcagcgcg cggggccagg tctcgcgctg gccgagcggc gtgcgcgacc agtcggtggt
      421 ctccagcagc acgcggggtg cgtcgggatc gtcggcagtc gagggagctt cggtcatgcg
      481 catccggggc ggcagctcgg ccatgttagc caccttcggc gtgatcgtcc gttcagatcg
      541 gtccgcatgg cagatttcgt gccccgcgac gccatcgagg aggggatgcg gcgcctttgt
      601 aggcgccgtc cgacgtccag cgaagcgcgc ggatacatgc gccggcgcga cgttacggtg
      661 ctcgaccgca atctgtcgtg ggtctgaaga cgggttgtcg gtttaccagt cttcacattg
      721 tttccctgtg caaggtgcac agttgtcggc accgcgccat tcgaagcgcg ccaccgagca
      781 atggagacga tcatgacagc agcacccttc atgcgtgcct tgcctttcgc cgtcgcgctg
      841 gcgctcgccg cctgcggtgg cggcggaggc ggcaccgaca cctcgaacaa cggcacgtcc
      901 agccccagca gcgccgacac cgccgtgtcg tcgggcgcca tctcggcgtt cggcagcgtc
      961 ttcgtcaacg gccacgagtt cgccacgggc tccgcgcgcg tgatggacga cgacaccggc
     1021 gacacggccg gccgggtctc agaattggaa gtcggccagg tggtcgacgt ggagccgtcg
     1081 agctccagca ccaccgccgc gcccgtcgcg caatggctgc atgtgcaccc gctggtgcgc
     1141 ggctacgtcg acgcgtccga cacgacggcc ggcaccatca ccgtgatggg ccaggtcgtg
     1201 agcctggaca gcggcacgct gttctccgac caccgcgcct gcgtcgctgc cacgacgaac
     1261 gcgtgcacgg ccgtcaccga cgcgtcgggc ctggtcgcga ccaccggcgg cacgccgggc
     1321 acgtacgcga ccgtcgacgg ctatctgttc agcagcaccg ccggctcggc gcagatcgtc
     1381 gccacgctgg tgtcggtgcg cgacgtcccc accagctacc cggcggcgtt caaggccgag
     1441 ggcgtggtcg gcgcgacgac cgtcggcggc gcgaccgcgg gcgtcaccat cggcggcctg
     1501 gcggtcgacc tggaccacgc cacctgccgc gtggatggca agccggtggc atgcgccacg
     1561 gcgttcgcgg ccggcgaggt cgtctcggtg ttctccgcga cggcgcccgc gctgcccgcc
     1621 accgccttca atgcgacggt cgcgatcgag cgcaggcacc tgccggtgca gacggccggc
     1681 caggcggtgg agttcgacgg ccgcgtgtcg gcgtcgaccc ccgcgtcggg cacgatggcg
     1741 gccaccttcg tcgtgcgcgg cgtgaccgtc gacacctcgc agatgccggc cggcacctcg
     1801 ctgcccgcgg tgggcgacat ggtgcgcgtg gcgggcacgg tgtcggccga cggcctgtcc
     1861 gtcgtggcca cgtcgatcaa gatcacccat gccgcgcgca gcgtcagcta tgcgctctcg
     1921 ggtgacgtca ccgcggtggc ggccggcacg gtcagcggca cgttcaacgt gacggtgctg
     1981 ggccagacga tcgtcgtcag cgaccagacg cgcctgtccg accgccagac ctggcagtgg
     2041 atggacgacg acccgcagag caatccgttc aacatcgcca cgttccagac ctatctcgcg
     2101 gcaagcgcgt cgcagcacgt ggcggtgaag gcggccaagg actccagcgg cgtgctgcag
     2161 gcgctgtcgt tcgcgatctg gccggcctcg acgttcagcg aggtcgcggg cccggtggac
     2221 gcgacgcccg cgc
//
LOCUS       DATCRO010000928         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_798351, whole genome shotgun sequence.
ACCESSION   DATCRO010000928 DATCRO010000000
VERSION     DATCRO010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798351"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1632
                     /locus_tag="VIN75_25240"
     CDS             136..1632
                     /locus_tag="VIN75_25240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8977543.1"
                     /translation="MTEAAQAQVPAQTDQAPEQKAPAPARFDSLPLDPKLLRAVADQG
                     YAFMTPIQAKAIPLVLQGRDVMGAAQTGTGKTAAFSIPLLQKMMRHETASVSPARHPV
                     RALVLAPTRELADQVAQNIKAYAKHSNLRVAVVFGGIDMKPQTLELKGGVEVLIATPG
                     RLLDHIEAKNCVLNQVEYVVLDEADRMLDIGFLPDLQRILSYLPKQRQTLLFSATFSP
                     EIKRLANSYLQDPVTVEVARANATASTVEQHFYSVTDDDKRAAVRQILKQRELRQAIV
                     FVNSKLGAARLARSFERDGLRTSALHGDKSQDERLKSLAAFKKNEVDVLVCTDVAARG
                     LDIADLPAVFNFDVPFNAEDYVHRIGRTGRAGASGLAVTLVTRDDARNVSDIEKLINK
                     KIELEPFEVEDDRPRRPPRPRFDDEPRRDREPSYAAPRSAPPMRAPAPQKSRDPFFDQ
                     PYEASTAVAEKPEWEKTAAAPAIGRPSISANIRAKRKVAALLGGGSKA"
     gene            complement(1658..2470)
                     /locus_tag="VIN75_25245"
     CDS             complement(1658..2470)
                     /locus_tag="VIN75_25245"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977544.1"
                     /translation="MTRPDLAVTLTDAPPAGYVALCWDVFFRSRGRGVSMQAHFPWLD
                     VPGQAVFATLESDGRVLAGCAMRFIEDDARRRRGATIGLVCVDENHRGLGHSSRVLER
                     AIAHADALGLADLVLWTGKPGVYERHGFAVDDDGLFGTVTAPGGPMSTALDATRTRWP
                     GHGETRGLPPFATGGSRWASALASAIVVDDGSGAILAQWQGPDDAVADLLAHAMPPAW
                     RLNALVGDGLPATLAARGFTLQLQPSRLRMVRPAPGSDATPRRYGLRLLDRI"
     gene            complement(2493..3413)
                     /locus_tag="VIN75_25250"
     CDS             complement(2493..3413)
                     /locus_tag="VIN75_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY8977545.1"
                     /translation="MNLSTPLRAALAAASRPLQGRLAQLVYPPGTQPGDFLQPAGEAA
                     LLDAGSVSWRVFANPVALLVGGVAAVLLELGEPRVRTGVWEHTTFRTAPLPRLQRTGY
                     AAMLTVFGARSRTEAMIRGINAAHARIAGRTPAGAAYRADDVELLTWVHATATFGFLQ
                     GYVRCVRPLDDAARDAFYAENQASARLYGVPSPPGSERELNALFDRMRPLLEPSPIVL
                     EFLAIMQTMPLLPRPARPLQRLLLRAAVQSLPADIRARLSLDGPAWRLGPWQWRVVRL
                     LGRAADHLDLPTLPAQLARRRLGAGPATGA"
BASE COUNT          584 a         1392 c         1342 g          454 t
ORIGIN      
        1 ttctagccgg tgcgggccgc ccggccgttc cccgggcgcc ggacgcccgc gcgagccccg
       61 ggcgggagcg ccccggcgac gagcggcggt caccgatcat gcgaagttca tccgtacaag
      121 cgataatggc ggttcatgac agaagcagca caagcccagg tcccggctca aacggaccaa
      181 gcccccgagc aaaaagcacc ggcccccgcc cgtttcgatt ccctgccgct cgaccccaag
      241 ctcctgcgcg ccgtcgccga ccagggctac gccttcatga cgccgatcca ggcgaaggcg
      301 atccccctgg tgctccaggg acgcgacgtg atgggcgccg cccagacggg caccggcaag
      361 accgccgcgt tctcgatccc gctgctgcag aagatgatgc gccacgagac ggccagcgtc
      421 tcgcccgccc gccaccccgt gcgcgcgctg gtgctcgcgc ccacgcgcga gctggccgac
      481 caggtcgccc agaacatcaa ggcctacgcc aagcactcca acctgcgtgt cgcggtcgtc
      541 ttcggcggca tcgacatgaa gccccagacg ctcgaactca agggcggcgt cgaggtgctc
      601 atcgccaccc ccggtcgcct gctcgaccac atcgaggcca agaactgcgt gctcaaccag
      661 gtcgagtacg tcgtgctcga cgaggccgac cgcatgctcg acatcggctt cctgcccgac
      721 ctgcagcgca tcctgagcta cctgcccaag cagcgccaga cgctgctgtt ctcggccacg
      781 ttctcgcccg agatcaagcg gctggccaac agctacctgc aggatccggt caccgtcgag
      841 gtggcgcgcg ccaacgcgac ggcgtccacc gtcgagcagc acttctacag cgtcacggac
      901 gacgacaagc gggccgccgt gcgccagatc ctcaagcagc gcgaactgcg ccaggcgatc
      961 gtcttcgtga actccaagct gggcgccgcg cgcctggcgc gatcgttcga gcgcgacggc
     1021 ctgcgcacct cggcgctgca cggcgacaag tcgcaggacg agcgcctgaa gtcgctggcc
     1081 gcgttcaaga agaacgaggt cgacgtgctg gtgtgcaccg acgtggccgc ccgcggcctg
     1141 gacatcgccg acctgcccgc cgtcttcaac ttcgacgtgc cgttcaacgc cgaggactac
     1201 gtgcaccgca tcggtcgcac cggccgcgcc ggcgcgtcgg gcctggcggt cacgctggtc
     1261 acgcgcgacg acgcccgcaa cgtctcggac atcgagaagc tgatcaacaa gaagatcgag
     1321 ctcgagccgt tcgaggtcga ggacgaccgt ccgcgccgtc cgccgcgccc gcgcttcgac
     1381 gacgagccgc gccgcgatcg cgagccgtcg tacgccgcgc cgcgttcggc gcccccgatg
     1441 cgcgcgccgg ccccgcagaa gagccgcgat ccgttcttcg accagccgta cgaggccagc
     1501 acggccgtgg ccgagaagcc cgagtgggag aagaccgcgg cggcgcccgc gataggccgt
     1561 ccgtcgatct cggccaacat ccgcgccaag cgcaaggtcg cggccctgct gggcggcggc
     1621 agcaaggcct gacggccggc ggccggcgcg cgcctcgtca gatcctgtcg agcaggcgca
     1681 ggccgtaacg ccgcggcgtc gcgtccgacc ccggcgccgg acgcaccatc cgcagccgcg
     1741 agggctgcaa ctgcagcgtg aagccgcgcg cggcgagcgt cgccggcagg ccgtcgccca
     1801 cgagcgcgtt caggcgccag gcgggcggca tcgcgtgcgc gagcaggtcg gcgacggcgt
     1861 cgtccgggcc ctgccattgc gcgaggatcg cgccgctccc atcgtccacc acgatcgccg
     1921 aggcgagggc ggacgcccag cgcgagccgc cggtggcgaa cggcggcagg ccgcgtgtct
     1981 cgccgtggcc cggccagcgg gtgcgggtcg cgtcgagcgc ggtggacatc ggaccgccgg
     2041 gcgcggtgac cgtgccgaag agcccgtcgt cgtccacggc gaatccgtgc cgttcgtaca
     2101 cgcccggctt gcccgtccac agcaccaggt ccgcgagacc cagcgcatct gcgtgagcga
     2161 tcgcgcgttc cagcacacgc gacgaatggc ccaggccgcg atggttctcg tcgacgcaga
     2221 ccaggccgat cgtcgcgccg cgccggcgcc tcgcgtcgtc ctcgatgaag cgcatcgcgc
     2281 agcccgcgag cacccggccg tccgactcga gcgtcgcgaa gacggcctgt ccgggcacgt
     2341 ccagccaggg gaaatgcgcc tgcatcgaga cgccgcgtcc gcgcgaccgg aagaacacgt
     2401 cccagcacag cgcgacgtag ccggccggtg gcgcgtcggt cagggtgacg gcgaggtcag
     2461 ggcgagtcat gcggcgatcg tagccggccg cgtcacgcgc ccgtcgccgg acccgcgccc
     2521 aggcggcggc gtgccagctg cgccggcagc gtcggcaggt ccaggtggtc ggcggcgcgg
     2581 cccagcaggc gcaccacgcg ccattgccag gggccgagcc gccaggccgg gccgtccagg
     2641 gacaggcgcg cacggatgtc ggccggcagc gactgcaccg ccgcgcgcag cagcaggcgc
     2701 tgcagcggtc gcgcgggccg cggcagcagc ggcatggtct gcatgatcgc caggaactcc
     2761 agcacgatgg gcgagggctc cagcagcggc cgcatgcggt cgaacagcgc attgagttcg
     2821 cgttccgagc cgggcggcga cggtacgccg tacaggcgcg ccgaggcctg gttctcggca
     2881 tagaaggcat cgcgcgccgc atcgtccagc gggcggacgc agcgcacgta gccttgcagg
     2941 aagccgaagg tcgcggtggc gtgcacccag gtcagcagct cgacgtcatc ggcgcgatag
     3001 gccgcgcccg ccggcgtgcg gcccgcgatg cgcgcgtggg cggcgttgat gccgcggatc
     3061 atcgcctcgg tgcggctgcg cgctccgaac accgtcagca tcgcggcgta gccggtgcgc
     3121 tgcagccgcg gcagcggtgc ggtgcgaaac gtcgtgtgtt cccagacgcc cgtgcgcacc
     3181 cgcggctcgc ccagttcgag cagcacggcc gccacgccgc ccaccagcag cgcgaccggg
     3241 ttggcgaaca cgcgccagga cacggagccg gcatccagca gcgccgcctc gcccgcgggc
     3301 tgcaggaagt cgcccggctg cgtgcccggc gggtagacca gctgcgcgag ccggccctgc
     3361 agcgggcggc tggctgcggc cagcgcggcg cgcaagggag tcgacaagtt cattcagcgt
     3421 acggacatcg ggggtttgca tgcgactgcg agaatgcacg ccgccgtgcc gctcgagcgc
     3481 gcggcgaatg ctcgagtgtc acgcttcggc gcgacagtcg acgtcgagac aacaatcaaa
     3541 ttatcggggt ggaaggatga gatcgagctg ggcaggcgtg cgcctgcggg acgcacgcgc
     3601 gcgagcgtgg tgcgtgacgg ggttgtggct gctgtcgccg ctggcgatgg cggcgtccgt
     3661 gccgtggacg ccaagcagct cggcgcgcca cgcgatcgag ctgctggtcg acgacggcgg
     3721 cctgccgctg accgtcagcc aatggccgct gccgcgcgac gccgtgcaac gc
//
LOCUS       DATCRO010000929         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_438107, whole genome shotgun sequence.
ACCESSION   DATCRO010000929 DATCRO010000000
VERSION     DATCRO010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438107"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VIN75_25255"
     CDS             <1..684
                     /locus_tag="VIN75_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8977546.1"
                     /translation="YDDETALIVNQLHNLGLKKIAVFKQNDAYGQAGLDGVNKALKAL
                     DLAPVAIGGFERNTVNVGEAVKTICAASPDGVVQIGSYKACAAFIRQARKAGYGGQFF
                     NVSFVGTKALSDELKDEALGVVVSQVMPYPFSPAVPLVRDFLDAVKASGGDATANYSS
                     LEGYVDARIFVEGLRRGRANTREGLASGLESLQHYDFDGFNLNFTRDHTASHFVELSM
                     LTGDGGVRR"
     gene            complement(773..1444)
                     /locus_tag="VIN75_25260"
     CDS             complement(773..1444)
                     /locus_tag="VIN75_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977547.1"
                     /translation="MTPGGGGTLFDRIGLRVLEHLEHHGGAALQPSADGAAGLGSGRV
                     VLVTSTNSGEGKSFVAQGLAAALADQQDGDVIWVDAAFGRPPADADNIFRRGAAGLSE
                     IMTLGSLQGLAPAGAGTDRLWRLGRGVLAQPALLQRPDAVAKAMMALRAGFALTVLDA
                     PALAGCGALLAQADACVMVVDSRRTERGEARRALMESRLGPDRLAGLVLNHRPRPVPR
                     WLGGS"
     gene            complement(1441..>2166)
                     /locus_tag="VIN75_25265"
     CDS             complement(1441..>2166)
                     /locus_tag="VIN75_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977548.1"
                     /translation="RQRGATAHRAGQQRSGDMNGGVSRPVWTLREAGGAPPRAAQRVI
                     AVPWRHPALILACALAGAGATAVLSQRQAAAPAAVALAAHGHVQPILPAPDVVASAPV
                     LAGAGGPGLRLDLELGPASAASPAEVVAQDDDARDVEDVDALLKQSPPAAGALAPRAL
                     DGVAPAPAGRGAGLAPGLLAGLLLGLLAAAARELRGDRMRSVREAEWALGAPVLGAIP
                     TLSARARGALLAPASRRAAADPA"
BASE COUNT          293 a          880 c          758 g          235 t
ORIGIN      
        1 tacgacgacg agacggcgct gatcgtcaac cagctgcaca acctgggcct caagaagatc
       61 gccgtcttca agcagaacga cgcctacggc caggccggcc tcgacggcgt caacaaggcg
      121 ctcaaggccc tcgacctggc ccccgtggcc atcggcggct tcgagcgcaa caccgtcaac
      181 gtgggcgagg ccgtcaagac gatctgcgcc gcatcgcccg acggcgtcgt gcagatcggc
      241 tcgtacaagg cctgcgccgc gttcatccgc caggcgcgca aggccggcta cggcggccag
      301 ttcttcaacg tgtcgttcgt cggcaccaag gcgctgtccg acgagctcaa ggacgaggcc
      361 ctcggcgtcg tcgtgagcca ggtgatgccc tacccgttct cgcccgcggt gcccctggtg
      421 cgcgacttcc tcgacgcggt caaggcctcc ggcggcgacg ccacggccaa ctactccagc
      481 ctcgagggct atgtcgacgc gcgcatcttc gtcgagggcc tgcgccgcgg ccgcgccaac
      541 acccgcgaag gcctggccag cggcctggag tcgctgcagc actacgactt cgacggcttc
      601 aacctgaact tcacgcgcga ccacacggcc tcgcacttcg tcgagctgtc gatgctcacc
      661 ggagacggcg gcgtgcgccg ctgacgcgac gggcgcctcg ccccgcgacg aacccggccc
      721 gctcgcgcgc gccgggtttt ttcatgggtg gtcgaacgcc agggttcacc gcctacgaac
      781 cgccgagcca gcgcggaaca ggccgaggac gatggttcaa caccaggccg gccagccgat
      841 ccggccccag ccgcgactcc atcagcgcgc gccgcgcctc gccgcgctcg gtgcggcgcg
      901 agtcgaccac catcacgcac gcatcggcct gcgccagcag cgcgccgcag ccggccagcg
      961 cgggcgcgtc cagcaccgtc agcgcgaacc ccgcgcgcag cgccatcatc gccttggcga
     1021 ccgcgtcggg ccgttgcagc agcgcgggct gcgcgagcac gccgcggccg agccgccaca
     1081 accggtcggt gcccgcgccc gccggtgcca ggccctgcag cgagcccagc gtcatgatct
     1141 ccgacagccc cgccgcgccg cggcgaaaga tgttgtcggc gtcggccggc ggccggccga
     1201 acgccgcgtc cacccagatc acgtcgccgt cctgctgatc ggccagcgcc gcggccaggc
     1261 cttgcgcgac gaagctcttg ccctcaccgg agttcgtgct cgtgacgagg acgacgcggc
     1321 cggacccgag gcccgcggcg ccgtcggccg acggctgcag cgcggcgccg ccgtggtgct
     1381 cgaggtgttc cagcacccgc agtccgatgc gatcgaacag cgtgccgccc ccgccgggcg
     1441 tcatgccggg tccgcggccg cgcgccgcga ggccggcgcc agcagcgccc cgcgggcgcg
     1501 ggccgacagc gtcgggatcg cgcccagcac cggcgcgccc agcgcccact cggcctcgcg
     1561 caccgagcgc atgcggtcgc cgcgcagctc gcgcgcggcc gcggccagca ggcccagcag
     1621 cagcccggcg agcagccccg gcgccaggcc ggcgccgcgc ccggccgggg ccggcgccac
     1681 gccgtccagc gctcgcggcg cgagcgcacc cgcggccggt ggcgactgct tcagcaaggc
     1741 atcgacgtcc tcgacgtcgc gggcgtcgtc gtcctgcgcc acgacctccg cgggcgaggc
     1801 cgccgaagcg ggcccgagct ccaggtccag ccgcaggccg gggccgcccg cgcctgccag
     1861 gaccggcgcc gaggcgacga cgtccggcgc cggcaggatc ggctgcacgt gcccgtgcgc
     1921 cgccaacgcc acggcagccg gcgcggcggc ctggcgctgc gacagcactg ccgtcgcgcc
     1981 cgctcccgcg agcgcgcagg cgaggatcag cgccggatgc cgccacggca ccgcgatgac
     2041 gcgctgcgcg gcgcgcggcg gcgcgccgcc ggcctcgcgc agcgtccaca cgggccggga
     2101 cacgccgccg ttcatgtcgc cgctccgctg ctggcccgcg cgatgagccg tagcgcctcg
     2161 ctggcg
//
LOCUS       DATCRO010000930        12924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1112569, whole genome shotgun sequence.
ACCESSION   DATCRO010000930 DATCRO010000000
VERSION     DATCRO010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112569"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /gene="mnmA"
                     /locus_tag="VIN75_25270"
     CDS             complement(<1..429)
                     /gene="mnmA"
                     /locus_tag="VIN75_25270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903961.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY8977549.1"
                     /translation="MSAKRVVVGLSGGVDSAVSAYLLKKQGHEVVGIFMKNWEDDDDD
                     EYCSTRQDFLDAASVADVLDIELEHVNFAAEYKDRVFAEFLREHRAGRTPNPDVLCNA
                     EIKFKAFLDHAMRLGAESIATGHYARVRRNESTGRHELLKG"
     gene            complement(474..983)
                     /locus_tag="VIN75_25275"
     CDS             complement(474..983)
                     /locus_tag="VIN75_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8977550.1"
                     /translation="MAADRWKPSVTVAAVIEKDGRYLMIEEMTRDGLRINNPAGGLEP
                     GESPLEGAVREALEETACHFTPEALLGVYMARNPDSERGPGVTYMRFAFCGTASDPVP
                     GRTLDSPVVRTLWMTFDEIVAATPQLRGPLVLQCIADHRAGRRLPLDAIYTHASVASP
                     QAGTATSEP"
     gene            1225..2355
                     /locus_tag="VIN75_25280"
     CDS             1225..2355
                     /locus_tag="VIN75_25280"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8977551.1"
                     /translation="MLIGVPLETAAGETRVAVTPETAKKLKAQGHVLRVQSGAGVAAS
                     ATDEAYVAAGAQIVDRVAALGADLVLKVRAPQSDELGLMKPGAVLVGMLNPFDRDGLA
                     RLAGAKLTSFALEAAPRTTRAQSMDVLSSQANIAGYKAVMIAADKYQRLFPMLMTAAG
                     TIKAARVVVLGVGVAGLQAIATAKRLGAVIEATDVRPSVKEQVESLGAKFIDVPYETA
                     EEKEAAEGVGGYARPMPPSWLERQKVEVAKRVAQADVVVTTALIPGRAAPVLVTEDMV
                     RSMKPGSVIVDIAAGRGPAGTDGNCPLTEAGRTVVKHGVTIVGETNLPALVAADSSSL
                     YARNVLDFLKLVVDKEAAFHLDLQDDIVAACLMTHEGEVKRA"
     gene            2403..2717
                     /locus_tag="VIN75_25285"
     CDS             2403..2717
                     /locus_tag="VIN75_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY8977552.1"
                     /translation="MDIVSPTIINLIIFVLAIYVGYHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTEGAFARTMGVLAVALAAVNVFGGFLVTRRMLEMFRKKERPAKDG
                     QA"
     gene            2714..2953
                     /locus_tag="VIN75_25290"
     CDS             2714..2953
                     /locus_tag="VIN75_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977553.1"
                     /translation="MTMPVPETPPRSPFWRVLQLIAGVALFLYALRLLSPMPDDRAGF
                     ILLVAILAAGSMALTDSIVGLVVAGLDKMQKKGNE"
     gene            2950..4374
                     /locus_tag="VIN75_25295"
     CDS             2950..4374
                     /locus_tag="VIN75_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY8977554.1"
                     /translation="MSLNLVTLLYLVASVCFIQALKGLSHPTTSIRGNVFGMVGMTIA
                     ALTTGALIWKLAGTPLGLGWVLLGVVVGGGYGAWRAKTVEMTKMPELVAFFHSMIGLA
                     AVFIAIAAVAEPSALEPGLARGEPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSGTY
                     KFRLFKGAPVQFAGQHFLNAALGVAMLACGIAFVATESPIAFGLMLALSFVLGVLIII
                     PIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNR
                     SFFSVILGGFGAEPGTAAAGATEQRPVKSGSADDAAFILGNAESVIIVPGYGLAVARA
                     QHAVKELAAKLTSRGVAVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMDEINGEFG
                     QADVAIVLGANDVVNPAALVKGSPIYGMPILEAYKARTVIVNKRSMAAGYAGLDNELF
                     YMDKTMMVFGDAKKVVEDMLKAVE"
     gene            4479..6152
                     /locus_tag="VIN75_25300"
     CDS             4479..6152
                     /locus_tag="VIN75_25300"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY8977555.1"
                     /translation="MERNWLKNYPEGVAPHVNAGEYASLVELLDNSFRKYADLPAYKF
                     MGKDFSYRLVDELSRAFAAYAQTLGLAQGDRVAIMMPNVPQYPVVVAGLLRAGLVVVN
                     VNPLYTPRELEHQLKDSGAKAIVIVENFATTLQEVLHAVPTKKIVLASMGDLLGLLKG
                     TLVNYVVRNVKKMVPAFDLPGAVRFNDAIAIGRRGTFKPVKVGPDDIAVLQYTGGTTG
                     VSKGAVLLHRNLVANILQSDAWYQPALSKVAKGTQIVTICALPLYHIFGFNTNMMLGL
                     RMGSCNLLIANPRDIPAMFKDLKRQPFHSLPAVNTLFNAMANHPDFATVDWSHLVMSV
                     GGGMAVQQATAQLWLEKTGCPICEGYGLSETSPAVSCNPVNTTAYSGTIGLPMPDTDI
                     ALLDDEGNEVGPGERGEIAVRGPQVMAGYWQRPDETAKVMTADGFFRTGDIGQVDERG
                     YFKIVDRKKDMILVSGFNVYPNEVEDVVALIPGVLECAAVGVPDDKAGEAVKIVIVKK
                     DPALTEADVRAWCETNLTGYKRPKIVEFRSDLPKTNVGKILRRELRDGK"
     gene            6191..6673
                     /locus_tag="VIN75_25305"
     CDS             6191..6673
                     /locus_tag="VIN75_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977556.1"
                     /translation="MTTTFADHYVRTDAGKAEIRAQTIKMSRPARNLLMVIDGSCSGD
                     AWLGKVNGSTPGDLEVLIAAGLVAPAAAAPGRTVHQRVPVEVAVRDWTYDALYTLLTH
                     EAKERFGLIKGYRLILKIEGCADLAGIQAVALEFVEHIRKAHGEDSAARFRKQLGATE
                     "
     gene            6670..7389
                     /locus_tag="VIN75_25310"
     CDS             6670..7389
                     /locus_tag="VIN75_25310"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY8977557.1"
                     /translation="MVKRLAIVGAMHEEIAALRPCLADVRTERRAGRDFLLGRLDGHD
                     VILVRCGIGKVAAATTTAVLLDAFDASALLFTGVAGGLGEDVRVGDIVVASTLLQHDM
                     NAEPLFPRWEVPLTGRARFGADAGWSTRLVQASHALAGANLHAHGAAIHHGLVISGDR
                     FVSTHAESDELRAALPDALAVEMEGAAVAQVCHDFGRPFGVVRTISDRADDAAHGDFQ
                     RFVCDVAAPYSRDIVRAALAG"
     gene            complement(7560..8519)
                     /locus_tag="VIN75_25315"
     CDS             complement(7560..8519)
                     /locus_tag="VIN75_25315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977558.1"
                     /translation="MSDPIVPTPGADALLAQADALHDDAPARALELLRAIDVAALDAG
                     RLGRLSFLLDHVTGEKFGLWDEAIAGLRAVVARAGEDVTPLILRHAAIAAHMAADDAL
                     AREWTQALAASADAPLDKARALVALGAVQFSASRLSAEAAGRQALHALQPLGDLLDAP
                     GAGLDAAFGAVTNNIASELVERPLPDLAQPQLRDALARAADLAQRFWARAGTWVNHER
                     ASYLSAMAANALGSGDAGAAHARAGLALLDEFDVDREQSVDRAFLELELAAGLRLAGQ
                     PGRPEALARALALAARFGEPWLDRWFSDRQARNEALAAHYGRQ"
     gene            8611..9351
                     /locus_tag="VIN75_25320"
     CDS             8611..9351
                     /locus_tag="VIN75_25320"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY8977559.1"
                     /translation="MSLRLFVESPLSAGLEFALPEDAARHVQVRRLQPGDDLRLFDGC
                     GGEWSAQVTRMGKRDVDVRVVAHEALERELPFQLEIALGMPANERMDALIEKATELGV
                     AAIQPLQTERSVLRLDGERAARRVAHWQAVAVGAAEQSGRTRVPVVAPVRGLAAWLAD
                     LPDDGHAGRLVMSTGSAPALARVVAPAAQGRWLALSGPEGGLTADEEAVAVARGFTRV
                     SLGSLILRADTAPLAWLAQMAVLLQRGA"
     gene            9431..10036
                     /locus_tag="VIN75_25325"
     CDS             9431..10036
                     /locus_tag="VIN75_25325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8977560.1"
                     /translation="MTIDVPSPIDLRLMSDARPWAEAALGKRPVRPEFFDAFAREIDR
                     DGARILELGSGPGFLVEHLLRARPGLEYTALDFSPAMHQLAAERLGERAARVRFVERS
                     FREPDWACGLGPFDVVVTHQAVHELRHKDYASALHAQVRPLLAAGGRYLVCDHYLGGD
                     GLSNDQLYMTVDQQREALQRAGFASVEALLVKGGLALHRAQ"
     gene            complement(10302..10772)
                     /locus_tag="VIN75_25330"
     CDS             complement(10302..10772)
                     /locus_tag="VIN75_25330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977561.1"
                     /translation="MSRNRSNTFLATLALCASFTALSANAADLPGRHPAYLHALTDLR
                     AARWNLEHRPADAAVSAQEDMAIVETDRAIREAKVAAMEDGKNIEDHPHEDANLDRPG
                     RLHHALELLQKAKDDVAREEDNPESRNLRNRIVEHTDKAIEATRRAIHDVEQGR"
     gene            complement(11024..11497)
                     /locus_tag="VIN75_25335"
     CDS             complement(11024..11497)
                     /locus_tag="VIN75_25335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977562.1"
                     /translation="MLIRPSKTLAATILALAAAFGSCGAQAQGNPQHPAYLHALSDLR
                     LAHWNLTHRGGDAAVTEQESIAVAETDHAIREATRAAELDAKPVGTADVHEDAQLDRA
                     GALHHAQDLLAAARQDIAEREDNPRAREIQGRIVEHIDRALEATRHAIFDVEHRR"
     gene            complement(11603..12922)
                     /locus_tag="VIN75_25340"
     CDS             complement(11603..12922)
                     /locus_tag="VIN75_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY8977563.1"
                     /translation="MEAGRGGVMVDENVEHAMRLNLHSRLAQRVLWPMVDGEYRDERD
                     LYELGRRINWSEWITPDETIRVDVTAQRSPLQSLNFAALRIKDAVCDVMREMAGARPS
                     VDTRFPDLAISLHVNETHAWLAIDLSGEALFKRGWREQQGEAPLKETLASTILYAAGW
                     HGRPDDGGLLDPTCGAGTIAIEAAQIACNVAPGLTRRFAFERLRPFLDYSRQWGQMQV
                     EARDAVVKPAVPIWAGDVSFRMTDFAARNAERAGVAHAIEFKTADALQRPAPADRGTI
                     VMNPPYGERIDAKGRGSAGAEAPRAPRTFAAPRTQDTPARDAGERPRGPGADAWAREN
                     PEAARRAARDVPQRGGREAAIVDGGSASNAGEFFAQLATHWKRQYSGWTAWVLSPDMK
                     LPSAMRLKESRRVPMWNGPIECRLFRFDMVKGSARTDGSPAPRDNDS"
BASE COUNT         1811 a         4470 c         4680 g         1963 t
ORIGIN      
        1 gcccttgagc agctcatggc gccccgtcga ctcgttgcgg cggacgcgcg cgtagtgtcc
       61 ggtggcgatg gactcggcgc ccaggcgcat cgcgtggtcg aggaaggcct tgaacttgat
      121 ctcggcgttg cacagcacgt ccgggttggg cgtgcggccg gcgcggtgct cgcgcaggaa
      181 ctcggcgaag acgcggtcct tgtactcggc ggcgaagttg acgtgctcga gctcgatgtc
      241 cagcacgtcg gcgacgctgg cggcgtccag gaagtcctgg cgcgtcgagc agtactcgtc
      301 gtcgtcatcg tcttcccagt tcttcatgaa gatgccgacg acctcgtgcc cctgcttctt
      361 caagaggtag gccgacaccg ccgaatccac accgccggac aggccgacca ccacgcgctt
      421 cgctgacatg gcctcgattg taggaaacgg ggccttgtcg cgccgcgaag gggtcaaggt
      481 tcggaagtgg ccgtcccggc ctggggcgag gcgacgctgg catgcgtgta gatcgcatcg
      541 agcggcaggc gccggccggc gcggtggtcg gcgatgcatt gcagcaccag cggcccgcgc
      601 agctgcggcg tggcggcgac gatctcgtcg aaggtcatcc agagcgtgcg cacgacgggg
      661 ctgtccagcg tgcgcccggg caccgggtcg ctggccgtgc cgcagaaggc aaaccgcatg
      721 taggtcacgc ccgggccgcg ctcggagtcg ggattgcgcg ccatgtagac gcccaggagc
      781 gcctcgggcg tgaagtggca ggcggtctct tccagcgcct cgcgcacggc gccttcgagg
      841 ggcgactcgc cgggctcgag gccgccggcg gggttgttga tgcgcaggcc gtcccgcgtc
      901 atctcctcga tcatcaggta gcgcccgtcc ttctcgatga cggccgccac ggtgacgctc
      961 ggcttccaac gatctgctgc catggcgggc gattgtggcg gcggcggcgc gaggccgcca
     1021 gcgaatccgt gggatggcgc ggcgggtgtg gcgttgcgtg cacgcctcac cggcccgagg
     1081 cgtgacacta aacgcgcagg ggcaatgtgt gtaagctgtg ccgctatcgc gcggggccgg
     1141 cccacggcac gggggcgcct gcccggtccg gctgccgggc ccggatcttg cgaccaggac
     1201 gacgaagttg gcgaggagac atccatgttg atcggtgttc cgctggagac ggcggcgggc
     1261 gagacgcgcg tggccgtgac gccggagacg gccaagaagc tcaaggcgca gggccatgtg
     1321 ctgcgggtgc agtcgggggc gggcgtcgcc gccagcgcca ccgacgaggc ctacgtggcc
     1381 gccggcgcgc agatcgtcga ccgcgtcgcc gccctcgggg ccgacctcgt cctgaaggtc
     1441 cgcgcgccgc agtccgacga gctgggcctg atgaagccgg gcgccgtgct ggtgggcatg
     1501 ctcaacccgt tcgaccgcga cgggctcgcc cgcctggccg gtgccaagct gacgtccttc
     1561 gccctggagg ccgccccgcg cacgacgcgg gcgcagagca tggacgtgct gtcctcgcag
     1621 gccaacatcg ccggctacaa ggccgtgatg atcgcggccg acaagtacca gcgcctgttc
     1681 ccgatgctga tgacggccgc cggcacgatc aaggccgcgc gcgtcgtcgt cctgggcgtg
     1741 ggcgtcgccg gcctgcaggc catcgccacg gccaagcgcc tgggcgcggt catcgaggcc
     1801 accgacgtgc ggcccagcgt caaggagcag gtcgagtcgc tcggcgccaa gttcatcgac
     1861 gtcccctacg agacggccga ggagaaggaa gccgcggagg gcgtgggcgg ctacgcgcgc
     1921 ccgatgccgc cgtcctggct ggaacgccag aaggtcgagg tcgccaagcg cgtggcccag
     1981 gccgatgtcg tcgtcaccac cgcgctgatc ccgggccgcg cggcgccggt gctcgtcacc
     2041 gaggacatgg tgaggtcgat gaagccgggc tcggtgatcg tggacatcgc cgccggccgc
     2101 ggtcccgccg gcaccgacgg caactgcccg ctgaccgagg ccggccgcac ggtcgtcaag
     2161 cacggcgtca cgatcgtcgg cgagaccaac ctgccggcgc tggtcgcggc ggactcgtcg
     2221 tcgctgtacg cgcgcaacgt gctcgacttc ctgaagctcg tggtcgacaa ggaagccgcg
     2281 ttccatctcg acctgcagga cgacatcgtc gccgcgtgcc tgatgacgca cgagggcgag
     2341 gtcaagcgcg cctgagacgc ctggccggcg ccgcaacgga tccaacgttt cgaggagtcc
     2401 ccatggacat cgtctccccc acgatcatca acctgatcat cttcgtgctg gcgatctacg
     2461 tcggctacca cgtggtgtgg aacgtcacgc cggcgctgca cacgccgctg atggcggtca
     2521 ccaacgcgat ctcggcgatc gtgatcgtcg gcgcgatgct ggccgcggcg ctcaccgagg
     2581 gcgcgttcgc caggaccatg ggcgtgctgg ccgtggcgct ggccgcggtc aacgtgttcg
     2641 gcggcttcct cgtgacgcgg cgcatgctgg agatgttccg caagaaggaa cgtcccgcca
     2701 aggacggcca ggcatgacga tgccggtgcc cgagacgccg ccgcgcagcc cgttctggcg
     2761 cgtcctgcaa ctgatcgcgg gcgtggcgct gttcctgtac gcgctgcggc tgctgtcgcc
     2821 gatgcccgat gaccgagcgg gcttcatcct gctggtggcg atcctcgccg cgggctcgat
     2881 ggcgctgacg gactcgatcg tcggcctggt cgtggccggc ctggacaaga tgcaaaagaa
     2941 ggggaacgaa tgagcctcaa cctcgtcacg ctgctgtacc tggtggccag cgtctgcttc
     3001 atccaggcgc tcaaggggct gtcgcatccg accacgtcga tccgcgggaa cgtgttcggc
     3061 atggtgggca tgaccatcgc cgcgctgacc accggcgcgc tgatctggaa gctcgcgggc
     3121 acgccgctgg gcctgggctg ggtgctgctg ggcgtggtgg tcggcggcgg ctacggcgca
     3181 tggcgcgcca agacggtcga gatgaccaag atgcccgagc tcgtggcgtt cttccacagc
     3241 atgatcggcc tggccgcggt gttcatcgcg atcgcggcgg tcgccgagcc gtcggccctc
     3301 gaacccggcc tggcccgcgg cgagcccatt cccaccggca accggctcga gctgttcctg
     3361 ggcgcggcca tcggcgccat cacgttcagc ggctcggtca tcgcgttcgg caagctctcg
     3421 ggcacctaca agttccgtct gttcaagggc gcgccggtgc agttcgcggg ccagcacttc
     3481 ctcaatgcgg cgctcggcgt ggcgatgctg gcgtgcggca tcgcgttcgt ggccacggag
     3541 agcccgatcg cgttcggcct gatgctcgcg ctgagcttcg tgctgggcgt gctgatcatc
     3601 atcccgatcg gcggcgcgga catgcccgtc gtcgtctcga tgctcaacag ctactcgggc
     3661 tgggcggcgg cgggcatcgg cttctcgctg aacaactcga tgctgatcat cgcgggctcg
     3721 ctggtgggca gctcgggcgc gatcctgagc tacatcatgt gcaaggcgat gaaccgctcg
     3781 ttcttcagcg tgatcctggg cggcttcggc gccgagcccg ggacggcggc ggccggcgcg
     3841 acggagcagc ggccggtcaa gagcggatcg gccgacgacg ccgctttcat cctgggcaac
     3901 gccgagagcg tgatcatcgt gccgggctac ggcctggcgg tggcgcgcgc gcagcacgcc
     3961 gtcaaggagc tggccgccaa gctcacgagc aggggcgtgg ccgtcaagta cgcgatccac
     4021 ccggtggccg gccgcatgcc gggccacatg aacgtgctgc tggccgaggc cgaggtgccc
     4081 tacgaccagg tgttcgagat ggacgagatc aacggcgagt tcggccaggc cgacgtcgcg
     4141 atcgtcctgg gcgccaacga cgtcgtgaac ccggcggcgc tcgtcaaggg ctcgccgatc
     4201 tatggcatgc ccatcctcga ggcctacaag gcgcgcacgg tcatcgtgaa caagcgttcc
     4261 atggccgccg gctacgccgg gctcgacaat gagctgttct acatggacaa gacgatgatg
     4321 gtcttcggcg acgccaagaa ggtggtcgag gacatgctca aggccgtcga gtgacgtgac
     4381 ttcccgtgcg tttggcgccc gaccgatcgg aagaatcgga taacgtccca ccacaagaat
     4441 ttcccttacc gctacagacc ccgcacgccg gagacaagat ggaacgcaac tggttgaaga
     4501 actaccctga gggggtcgcg ccgcacgtga acgctggcga atacgcgtcg ctggtcgaac
     4561 tgctggacaa cagctttcgc aagtacgccg acctgcccgc ttacaagttc atgggcaagg
     4621 acttcagcta ccggctggtc gacgagctgt cgcgcgcctt cgcggcctac gcgcagacgc
     4681 tgggcctggc gcagggcgac cgcgtcgcca tcatgatgcc caacgtgccg cagtacccgg
     4741 tcgtcgtcgc cggcctgctg cgcgccggcc tcgtggtggt caacgtcaac ccgctgtaca
     4801 cgccgcgcga gctggagcac cagctcaagg actccggcgc caaggccatc gtcatcgtcg
     4861 agaacttcgc caccacgctg caggaagtgc tgcacgcggt gcccaccaag aagatcgtcc
     4921 ttgcctccat gggcgacctg ctgggcctgc tcaagggcac gctggtcaac tacgtcgtgc
     4981 gcaacgtcaa gaagatggtg ccggcgttcg acctgcccgg cgccgtgcgc ttcaacgatg
     5041 cgatcgcgat cggccgccgc ggcacgttca agcccgtcaa ggtagggccg gacgacatcg
     5101 ccgtgctgca gtacaccggc ggtaccacgg gcgtgagcaa gggcgcggtg ctgctgcatc
     5161 gcaacctggt ggccaacatc ctgcagtcgg acgcctggta ccagccggcg ctgagcaagg
     5221 tggccaaggg cacgcagatc gtcaccatct gcgcgctgcc gctgtatcac atcttcggct
     5281 tcaacacgaa catgatgctg ggcctgcgca tgggctcgtg caacctgctg atcgccaatc
     5341 cgcgcgacat ccccgcgatg ttcaaggatc tcaagcgcca gccgttccac agcctcccgg
     5401 ccgtcaacac gctgttcaac gcgatggcga atcacccgga cttcgcgacg gtcgactggt
     5461 cgcacctcgt gatgtccgtg ggcggcggca tggccgtgca gcaggccacg gcgcagctgt
     5521 ggctggagaa gacgggctgc ccgatctgcg agggctacgg cctgtccgag acctcgcccg
     5581 cggtgtcgtg caatccggtc aacacgactg cgtacagcgg caccatcggc ctgcccatgc
     5641 ccgacaccga catcgcgctg ctggacgacg agggcaacga ggtgggcccc ggcgagcgcg
     5701 gcgagatcgc cgtgcgcggc ccgcaggtga tggccggcta ctggcagcgc cctgacgaga
     5761 ccgccaaggt catgaccgcc gacggcttct tccgcaccgg cgacatcggc caggtggacg
     5821 agcgcggcta cttcaagatc gtcgaccgca agaaggacat gatcctggtc tcgggcttca
     5881 acgtgtaccc caacgaggtc gaggatgtcg tggcgctgat cccgggcgtc ctggaatgcg
     5941 ccgcggtggg cgtgccggac gacaaggccg gcgaggccgt caagatcgtc atcgtcaaga
     6001 aggacccggc gctgaccgag gccgacgtgc gggcctggtg tgaaacaaat cttaccggtt
     6061 acaagcgccc caagatcgtt gaattccgtt ctgatctgcc gaaaaccaat gtagggaaga
     6121 tcctgcggcg cgagctgcgg gacggcaaat gacgatctag cgggcctcgg gccgtcagga
     6181 aacctcgatg atgaccacca ccttcgccga tcactacgtc cgcaccgacg ctggcaaggc
     6241 ggagatccgt gcgcagacga tcaagatgtc gcgcccggcg cgcaacctgc tgatggtcat
     6301 cgacggcagc tgcagcggcg acgcgtggct gggcaaggtc aacggcagca cgccgggcga
     6361 cctggaggtg ctgatcgcgg ccgggctggt cgcgcccgcc gccgccgcgc cggggcgcac
     6421 ggtgcaccag cgcgttccgg tggaggtcgc ggttcgcgac tggacctacg acgcgctgta
     6481 cacgctgctc acgcacgagg ccaaggagcg cttcggcctc atcaagggct accgcctcat
     6541 cctcaagatc gagggctgcg ccgacctggc cggcatccag gccgtggcgc tggagttcgt
     6601 cgagcacatc cgcaaggcgc acggcgagga ctcggccgcg cgcttccgca agcagctggg
     6661 cgccaccgaa tagtgaagcg cctcgccatc gtcggcgcga tgcacgagga gatcgccgcg
     6721 ttgcggccct gcctggccga tgtgcgcacg gaacggcgcg ccggccgcga tttcctcctc
     6781 ggccgcctcg acggccacga cgtgatcctg gtgcgctgcg gcatcggcaa ggtggctgcg
     6841 gccaccacca ccgcggtgct gctggacgcc ttcgacgcga gcgcgctgct gttcaccggc
     6901 gtggcgggtg gcctgggcga ggacgtgcgc gtcggcgaca tcgtcgtggc cagcacgctg
     6961 ctgcagcatg acatgaacgc cgagccgctg ttcccgcgct gggaggtgcc gctcaccggc
     7021 cgcgcgcgct tcggcgcgga cgcgggctgg tcgacccgac tcgtgcaggc gagccacgct
     7081 ctggcgggcg cgaacctgca cgcgcacggc gcggcgatcc accacggcct ggtcatcagc
     7141 ggcgatcgct tcgtgtcgac gcacgcggag agcgacgagt tgcgcgccgc actgccggat
     7201 gcgctggccg tggagatgga aggcgccgcc gtggcccagg tctgccatga cttcggacgg
     7261 ccgttcggcg tcgtgcgcac catctccgac cgcgccgacg atgccgctca cggcgatttc
     7321 cagcgtttcg tgtgcgatgt ggccgcgccg tattcgcgcg atatcgtgcg cgccgcattg
     7381 gccgggtagg accggtcagg ggccgccggg cggcgctcga tgaagtcgaa gaaggcgccc
     7441 ccgaccgacg ggccgtcatc ctgcgcgtag cgaagcggag tcgcaggatc cacggttgcg
     7501 tgccggtgcg cgccctggcc gggaaaggtg gatcctgcga ctgcgcgcag cagcgcggat
     7561 cattgcctgc cgtagtgtgc cgccaacgcc tcgttgcgag cctgccggtc cgagaaccag
     7621 cggtccagcc acggctcgcc gaaccgggcc gcgagcgcca gcgcgcgggc cagcgcctcc
     7681 gggcggccgg gctgccccgc caggcgcagg cccgcggcga gctccagctc caggaaggcg
     7741 cggtcgacgg actgttctcg atcgacgtcg aactcgtcca gcagcgccag ccccgcgcgc
     7801 gcatgcgcgg cgcccgcgtc gccgcttccc agcgcattgg cggccatcgc gctgaggtag
     7861 ctcgcacgct cgtggttgac ccacgtgccc gcacgcgccc agaagcgttg cgccaggtcg
     7921 gcggcgcgcg cgagcgcgtc gcgcagctgc ggttgcgcga ggtcgggcag gggacgttcg
     7981 acgagctcgg aggcgatgtt gttggtgacc gcgccgaacg ccgcgtccag gccggcgccg
     8041 ggcgcgtcca gcaggtcgcc cagcggctgc agcgcgtgca gcgcctggcg ccccgccgcc
     8101 tcggccgaca ggcggctggc cgagaactgc accgccccca gggcgaccag cgcgcgcgcc
     8161 ttgtccagcg gtgcgtcggc gctcgcggcc agcgcctgcg tccactcgcg cgccagcgcg
     8221 tcgtcggccg ccatgtgcgc ggcgatggcg gcgtggcgca ggatcagcgg cgtgacgtcc
     8281 tcgcctgcgc gggcaacgac ggcgcgcagc ccggcgatcg cctcgtccca caggccgaac
     8341 ttctcgccgg tgacgtgatc cagcaggaag gacaggcggc ccagccggcc ggcgtccagt
     8401 gcggcgacgt cgatcgcgcg cagcagctcg agcgcgcgcg cgggcgcgtc gtcgtgcagg
     8461 gcgtcggcct gcgcgagcag cgcgtcggcg ccgggcgtgg ggacgatggg gtcgctcatg
     8521 gtggggccgg ggaagtggcg cgcccatcgt agcgcggcgc cggcggcggc atgcggcaca
     8581 ctgcaggttg tcgtccgccg tcccagcccc atgtccctgc gcctgttcgt cgaatccccc
     8641 ttgtccgccg gcctcgagtt cgcgctgccg gaggacgccg cgcgccacgt ccaggtgcgg
     8701 cggctgcagc ccggcgacga cctgcgcctg ttcgacggct gcggtggcga gtggtcggcg
     8761 caggtcacgc gcatgggcaa gcgcgacgtc gacgtgcgcg tcgttgcgca cgaggcgctc
     8821 gaacgcgaac tgccgttcca gctcgagatc gcgctgggca tgcccgccaa cgagcgcatg
     8881 gacgcgctga tcgagaaagc cacggagctc ggtgtcgccg cgatccagcc gttgcagacc
     8941 gagcgctcgg tgctgcgcct ggacggcgaa cgcgcggcca ggcgcgttgc gcactggcag
     9001 gccgtggccg tgggcgccgc cgaacagagc gggcgcacgc gcgtgcccgt cgtcgcgccg
     9061 gtgcgcggcc tcgcggcgtg gctcgccgac ttgccggacg acggccatgc cgggcgcttg
     9121 gtcatgagca ccggcagcgc gccggcgctg gcgcgcgtcg tcgcgcccgc cgcgcagggc
     9181 cgctggctgg cgttgagcgg ccccgagggc gggctcacgg ccgacgagga agccgtcgcc
     9241 gtcgcgcgcg gcttcacgcg cgtcagcctg ggctcgctga tcctgcgcgc cgacactgcg
     9301 ccgctcgcct ggctcgcgca gatggccgtg ctgctgcagc gcggcgcgtg aagactttcg
     9361 ccgcgaagcg cgaaggcttc ttcgtggcgc tgcgggcaga ctgcacgatc ctcgattcaa
     9421 cggagccgac atgaccatcg acgtccccag cccgatcgac ctgcgcctga tgtccgacgc
     9481 ccggccctgg gccgaggccg cgctcggcaa gcgccccgtg cgccccgaat tcttcgacgc
     9541 cttcgcccgc gagatcgacc gcgacggcgc acgaatcctc gaactcggct cgggccccgg
     9601 cttcctcgtc gagcacctgc tgcgcgcccg gcccgggctc gagtacacgg ccctcgattt
     9661 ctcgccggcg atgcatcagc tcgccgccga gcgtctgggc gagcgcgcgg cgcgcgtgcg
     9721 cttcgtcgag cgcagcttcc gcgagccgga ctgggcctgt ggcctgggcc cgttcgacgt
     9781 cgtcgtcacg caccaggcgg tgcacgagct gcgccacaag gactacgcca gcgcgctgca
     9841 tgcgcaggtc cgcccgctgc tggccgccgg cggacgctac ctcgtctgcg accactacct
     9901 gggcggcgac ggcctgtcca acgaccagct gtacatgacc gtcgaccagc agcgcgaggc
     9961 cttgcagcgc gcggggttcg cgagcgtcga ggcgctgctg gtcaagggcg ggctggcgct
    10021 gcatcgggcg caatagatca gcgatggcca cacgacggcg tggcgcggtg ggaggggtcg
    10081 ggcctgtcct cgaaatcgcg ctgacgcgcc accgcgagcc gaggttcccc ccgcgattcg
    10141 aggcgaaggc ctgacctcga tcatcggctg acgctcgggg tcaggccttc gccccatgtc
    10201 gccgctggcg cccctgccgt cggctgcgtc ccggcggcaa catgaggaga ggccagaccc
    10261 ctcgcgcagc gcggcggaaa tcgagttcgc gcctcgtccc ctcaacgccc ttgttcgacg
    10321 tcgtggatcg cgcggcgcgt cgcctcgatg gccttgtcgg tgtgttcgac gatgcggttg
    10381 cgcaggttgc gcgactcggg gttgtcttcc tcgcgggcca catcatcctt ggccttctgc
    10441 agcagctcca gcgcgtggtg caggcggccc gggcggtcga ggttggcgtc ctcgtgcggg
    10501 tggtcctcga tgttcttgcc gtcctccatc gcggcgacct tggcctcgcg gatggcgcgg
    10561 tcggtctcga cgatggccat gtcctcctgg gcgctcacgg cggcgtcggc ggggcgatgc
    10621 tcgaggttcc agcgtgccgc acgcaggtcg gtcagcgcgt gcaggtaggc gggatggcgg
    10681 ccgggcaggt cggcggcgtt ggccgacagc gcggtgaacg acgcgcagag cgcgagcgtg
    10741 gcgaggaagg tgttcgagcg attgcgggac atcctggtct cctggtttgt gggaacgtgt
    10801 gcgtggtgcg cacccaggaa acggcccgct cgaaacggtc gttgacgctg cacggcgccg
    10861 ctggcttgcg cggtcctgtc gtcaggatga cgcgcgacgg gccgcgatgt cggttgcggg
    10921 aacgggcggc ctgcacagtc catggcacgc gcgcgccatg aagaacgccg cgcggaccgg
    10981 ggccggcgcg gcgtcatgcg ggcgcctcgc ggcgcgtgtc cggtcaacgg cgatgttcga
    11041 cgtcgaagat cgcgtggcgc gtcgcctcga gcgcgcggtc gatgtgttcg acgatgcgcc
    11101 cctggatctc gcgtgcccgc gggttgtcct cgcgctcggc gatgtcctgg cgcgccgcgg
    11161 cgagcaggtc ctgcgcgtgg tgcagcgcgc ccgcgcggtc gagctgcgcg tcctcgtgaa
    11221 cgtcggcggt gcccacgggc ttggcgtcga gctccgccgc gcgcgtggcc tcgcggatcg
    11281 cgtggtcggt ttccgcgacg gcgatgcttt cctgctcagt gacggccgca tcgccgccgc
    11341 ggtgcgtcag gttccagtgc gcgagccgca ggtccgacag cgcgtgcaga taggccgggt
    11401 gttgcgggtt gccctgggcc tgggcgccgc acgagccgaa ggcggcggcg agcgcgagga
    11461 tcgtggcggc cagggtcttc gaaggacgga tgagcatgac ggtctccttg tcgagggtcg
    11521 ttttcgcccg gttgtcctgc aagaacgacg ccggctcggg cctcgttgac cgccacgtcc
    11581 gtggatgagc gtgtgagcgt cgttacgaat cgttgtcgcg gggcgcgggc gagccgtccg
    11641 tgcgcgccga gcccttgacc atgtcgaacc ggaacaagcg gcattcgatg ggcccgttcc
    11701 acatcggcac gcgccggctc tccttgagcc gcatcgcgct cggcagcttc atgtcggggc
    11761 tcagcaccca cgccgtccag ccgctgtact gtcgcttcca gtgggtcgcg agctgcgcga
    11821 agaactcgcc ggcgttcgac gcgctgccgc cgtcgacgat cgccgcttcg cggcccccgc
    11881 gctgcggcac gtcgcgcgcg gcacggcgcg cggcctcggg gttctcgcgc gcccacgcgt
    11941 ccgcgcccgg gccgcgcggg cgctcgcccg cgtcacgcgc gggggtgtcc tgcgtgcgcg
    12001 gggccgcgaa ggtgcgcggc gcgcgcggcg cctcggcgcc cgcgctgccg cggcccttgg
    12061 cgtcgatgcg ctcgccgtac ggcgggttca tcacgatggt gccgcggtcg gccggcgcgg
    12121 ggcgctgcag cgcgtcggcc gtcttgaact cgatggcgtg cgccacgccc gcgcgctcgg
    12181 cgttgcgggc ggcgaagtcg gtcatgcgga acgacacgtc gccggcccag atcggcacgg
    12241 ccggcttgac gacggcatcg cgcgcctcga cctgcatctg gccccattgc ctcgagtagt
    12301 ccaggaacgg gcgcagccgt tcgaacgcga agcgccgcgt gagccctggc gcgacattgc
    12361 aggcgatctg cgcggcctcg atcgcgatcg tgccggcgcc gcaggtggga tcgagcagcc
    12421 cgccgtcgtc gggccggcca tgccagccgg ccgcgtagag gatcgtggac gccagcgtct
    12481 ccttcagcgg cgcctcgccc tgctgttcgc gccagccgcg cttgaacagc gcctcgccgc
    12541 tgagatcgat cgccagccac gcgtgcgtct cgttgacgtg caggctgatc gccaggtcgg
    12601 ggaagcgcgt gtcgacgctg ggccgcgcgc ccgccatctc gcgcatgacg tcgcagacgg
    12661 cgtccttgat gcgcagcgcg gcgaagttga gcgattgcag cggcgagcgt tgcgcggtca
    12721 cgtcgacgcg gatcgtctcg tcgggcgtga tccattccga ccagttgatg cggcggccga
    12781 gctcgtacag gtcgcgctcg tcacggtact cgccgtccac catcggccag agcacccgtt
    12841 gcgcgaggcg gctgtgcagg ttcaggcgca tcgcgtgttc cacgttctcg tcgaccatca
    12901 cgccgccgcg gccggcctcg atgc
//
LOCUS       DATCRO010000931         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_179332, whole genome shotgun sequence.
ACCESSION   DATCRO010000931 DATCRO010000000
VERSION     DATCRO010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179332"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="ybeY"
                     /locus_tag="VIN75_25345"
     CDS             complement(<1..440)
                     /gene="ybeY"
                     /locus_tag="VIN75_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8977564.1"
                     /translation="MARAPLPELQLSLQFADPADRALLPRHKVARWIRAALEAPGEIT
                     VRVVDADEGRRLNREFRGKDYATNVLTFDYVQEPEIEADLVLCAPVVEEEARKQKIDL
                     VAHYAHLLVHGTLHAQGYDHEVDDEAACMEARETEVLAGLGFGDP"
     gene            complement(440..1492)
                     /locus_tag="VIN75_25350"
     CDS             complement(440..1492)
                     /locus_tag="VIN75_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8977565.1"
                     /translation="MIVRRAFSPPDNERLAHVCGVLDENLRAIEQGLNVVITRRQEHF
                     RVEGGKLQAPRAMALVEALYERSGRPIDEEQLQLMLHDAQRDVIQGHARPVPQEEATA
                     GGEIVLHTRRANLIGRTPNQVQYLRQILSHDITLGLGPAGTGKTFLAVACAVDALERA
                     SVQRIVLTRPAVEAGERLGFLPGDLSQKVDPYLRPLYDALHDLMGYERVTKAFERGAI
                     EIAPLAFMRGRTLNHAFVILDEAQNATPEQMKMFLTRIGFGTKAVVTGDLTQVDLPRG
                     ARSGLADASAILRDVKGIAVTQFGATDVVRHPLVARIVEAYEAAAVRNAAAVEPPAVV
                     PASFQEGRRGTRGGRS"
     gene            1552..2124
                     /gene="ruvA"
                     /locus_tag="VIN75_25355"
     CDS             1552..2124
                     /gene="ruvA"
                     /locus_tag="VIN75_25355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8977566.1"
                     /translation="MIGRLTGIVAEKSPPQVLLDVQGVGYEVDVPMSTFCNLPGLGER
                     VTLLTHFVVREDAQLLFAFLTDEERATFRLLVKISGIGPRTALAILSGLSVADLAGAV
                     ARQEGGRLQKVPGIGKKTAERLLLELKGKLGPEIAAAGVAIRSDAQGDIVQALIALGY
                     NEKEAALAIKGLPKDVGVADGIKLAMKALS"
     gene            complement(2335..2757)
                     /locus_tag="VIN75_25360"
     CDS             complement(2335..2757)
                     /locus_tag="VIN75_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY8977567.1"
                     /translation="MIKNALIAAAVFASAAAHAQVAVSDPWVRTTVAQQKTTAAYMTI
                     TSLKGGKLVDASSPVAASAEVHEMKMDGDLMQMRAVDALPLPTGQAVELKPNGYHMML
                     MGLKAPVKAGDVVPIKLVIEDSKGQRKTVDVKAVARQN"
     gene            2960..4042
                     /gene="ruvB"
                     /locus_tag="VIN75_25365"
     CDS             2960..4042
                     /gene="ruvB"
                     /locus_tag="VIN75_25365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8977568.1"
                     /translation="MSIQTDDFSGSGARDPRPARVVGANPASPNEEALERALRPKRLD
                     DYVGQAKAREQLDIFIGAARKRAESLDHVLLFGPPGLGKTTLSHIIAAELGVNLRQTS
                     GPVLEKPKDLAAILTGLERNDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGDGPAA
                     RSIKLDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTSDELAAIVARSAGLLSVP
                     IDPDGALELARRSRGTPRIANRLLRRVRDYADVKAQGRITVPVANAALAMLDVDPQGF
                     DLMDRKLLEAIVHRFDGGPVGLDNVAAAIGEEPGTIEDVIEPYLIQQGYLQRTPRGRI
                     ATVAAYRHLGLAPPARTGNDLFGMPP"
     gene            complement(4060..4761)
                     /locus_tag="VIN75_25370"
     CDS             complement(4060..4761)
                     /locus_tag="VIN75_25370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8977569.1"
                     /translation="MSHGEATRLIAVRHGETAWNVEARLQGQLDIPLNDRGREQALRT
                     AHWLADDAPDIVVSSDLARAQATAHAIASFNRIPLELDTGLRERHFGSFQGMTHTEVA
                     EQWPALAARWKSRDPEFAPGVTGDGESLTVFYARCVDAALRIAMRHAGKTVVLVAHGG
                     VLDCLYRAATRVPLDAPRLWQLDNAAINRLLHTSSGLTMVGWNDTSHLDLNAPTLDES
                     ADRMPAAAALAARAD"
     gene            complement(4782..5087)
                     /locus_tag="VIN75_25375"
     CDS             complement(4782..5087)
                     /locus_tag="VIN75_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY8977570.1"
                     /translation="MIYKFKSRATGDLIMTQPVGERVLGLIGKERTPQGIIEVGQMPA
                     AIGALESAVAAESPRAAGDDDDAGGTGDRVSLRQRVWPMVEMMKRAMAEKEPITWGT"
     gene            complement(5193..>5451)
                     /locus_tag="VIN75_25380"
     CDS             complement(5193..>5451)
                     /locus_tag="VIN75_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977571.1"
                     /translation="DGLQSAYGDAKPLFSRATQRARELAHDGYDSARERAAALRERGA
                     QAVESTRGYVKDEPIKSLLIAAAVGAAVIALVEVVRIRRNR"
BASE COUNT          748 a         1931 c         1929 g          843 t
ORIGIN      
        1 ggatcgccga agcccagccc cgcgagcact tcggtctcgc gcgcttccat gcacgcggcc
       61 tcgtcgtcga cctcgtggtc gtagccctgc gcgtgcagcg tgccgtgcac cagcaggtgc
      121 gcgtagtggg cgacgaggtc gatcttctgc ttgcgggctt cctcctccac gaccggcgcg
      181 cacagcacga ggtcggcctc gatctcgggc tcctgcacgt agtcgaaggt cagcacgttg
      241 gtcgcgtagt ccttgccgcg gaactcgcgg ttcagccggc ggccttcgtc ggcgtcgacg
      301 acgcgcaccg tgatctcgcc cggcgcctcg agcgcggcgc ggatccagcg cgcgaccttg
      361 tggcgcggca gcagcgcgcg gtcggcgggg tcggcgaact gcaggctcag ctgcagctcg
      421 gggagcgggg cgcgggccat caggaacgtc ctccgcgggt gccgcgccgg ccttcctgga
      481 agctggccgg aacgacggcc ggcggctcga ccgcagcggc gttgcgcacg gcggccgcct
      541 cgtaggcctc gacgatgcgc gccaccagcg gatggcgcac gacgtcggtc gcgccgaact
      601 gcgtcaccgc gatgcccttg acgtcgcgca ggatggcact ggcgtcggcc aggccgctgc
      661 gcgcgccgcg cggcaggtcg acctgcgtga ggtcgccggt gacgacggcc ttggtgccga
      721 agccgatgcg cgtcaggaac atcttcatct gctcgggcgt ggcattctgc gcctcgtcga
      781 ggatgacgaa cgcgtggttg agcgtgcggc cgcgcatgaa ggccagcggc gcgatctcga
      841 tggcgccgcg ctcgaaggcc ttggtcacgc gctcgtagcc catcaggtcg tgcagcgcgt
      901 cgtacagcgg gcgcaggtag gggtcgacct tctgggacag gtcgcccggc aggaagccca
      961 ggcgctcgcc ggcctcgacc gcggggcgcg tcagcacgat gcgctgtacg ctggcgcgct
     1021 cgagcgcatc gacggcgcag gccacggcca ggaaggtctt gccggtgccg gccggaccca
     1081 ggccgagcgt gatgtcgtgc gacaggatct gcctcaggta ctgcacctgg ttgggcgtgc
     1141 ggccgatcag gttggcgcgc cgcgtgtgca gcacgatctc gccgccggcg gtcgcctctt
     1201 cctgcggcac cggcctggcg tggccctgga tcacgtcgcg ctgggcgtcg tgcagcatca
     1261 gctgcagctg ctcctcgtcg atggggcggc cgctgcgttc gtacagcgcc tcgaccagcg
     1321 ccatcgcgcg cggcgcctgc agcttgccgc cctcgacgcg gaagtgttcc tggcgccggg
     1381 tgatgacgac gttgagcccc tgctcgatcg cgcgcaggtt ctcgtcgagg acgccgcaca
     1441 cgtgggcgag gcgttcgttg tcgggtgggg agaaggcgcg gcggacgatc actggaggtt
     1501 tcgaagttct tggtgcgagg cgcgattgtc cgcccatccg ataatcaccg catgatcgga
     1561 cgtctcacgg gcatcgtcgc ggaaaagtcg cccccgcagg tactgctgga cgtgcagggc
     1621 gtcggctacg aggtggacgt gccgatgagc acgttctgca acctccccgg gctgggcgag
     1681 cgcgtcacgc tgctcacgca tttcgtggtg cgcgaggacg cgcagctgct gttcgccttc
     1741 ctcacggacg aggaacgcgc caccttccgc ctgctggtca agatctcggg catcggcccc
     1801 cgcacggcgc tggcgatcct gtcgggactg tcggtggccg acctcgccgg cgcggtcgcg
     1861 cgccaggagg gcgggcgcct gcagaaggtg ccgggcatcg gcaagaagac ggccgaacgc
     1921 ctgctgctgg agctcaaggg caagctgggc cccgagatcg cggccgccgg cgtcgccatc
     1981 cggagcgatg cgcaaggcga catcgtgcag gcgctgatcg cgctgggcta caacgagaag
     2041 gaagcggcgc tggcgatcaa gggcttgccc aaggacgtgg gcgtggccga cggcatcaag
     2101 ctggcgatga aggccttgtc gtaggcatcg gcggccggcg tcgccctgcg gtcgccgttc
     2161 tcggcatgcg ccgctgcgtc aagcaggcgt catcctgcgc gcagtcgcag gatccacctc
     2221 cgtcatgcgg ctccccggcc aacgtggatc ctgcgactgc gcccaggatg acacttcatg
     2281 ggcgctgcgc ggcgccagtt ccccggggga accgcctcac gggcaagcgc gccgtcagtt
     2341 ctgccgcgcg accgccttca cgtcgacggt cttgcgctgc cccttcgaat cctcgatcac
     2401 cagcttgatc ggcaccacgt cgccagcctt caccggcgcc ttcaggccca tcagcatcat
     2461 gtgatagccg ttgggcttga gttcgaccgc ctggcccgtg ggcagcggca gcgcgtcgac
     2521 ggcgcgcatc tgcatcaggt cgccgtccat cttcatctcg tgcacctcgg cggaggccgc
     2581 gacgggcgag ctggcgtcga ccagcttgcc gcccttcagc gaggtgatcg tcatgtaggc
     2641 ggcggtggtc ttctgctggg cgacggtggt gcgcacccac gggtcggaga cggcgacctg
     2701 ggcgtgggcg gcggcgctcg cgaagactgc ggcggcgatg agggcgttct tgatcatggt
     2761 gccggatcgt acgtgagtcg ggttcgaggc gcggcgacgc caaacgttcc cggcgctgcc
     2821 gctgatttcg cgccctgaag ttcgagggag cggcggatcc ggctctgccg ggccgctcgc
     2881 ggccggcccc tcggggcagg gagcgacagc gaccgccgcg aagcgcccct ccggggggtg
     2941 gggcagttat gattactgaa tgtccataca gactgacgat ttcagcggct ccggcgctcg
     3001 cgatccgcgt ccggcgcggg tcgtcggcgc caacccggcg tcgcccaacg aggaggcgct
     3061 ggagcgcgcc ctgcgaccca agcgactgga cgactacgtc gggcaggcca aggcgcgcga
     3121 acagctggac atcttcatcg gcgccgcccg caagcgcgcc gaatcgctgg accacgtgct
     3181 gctgttcggc ccgccgggcc tgggcaagac gacgctgtcg cacatcatcg cggccgaact
     3241 gggcgtgaac ctgcgccaga cctcgggccc ggtgctggaa aagccgaagg acctggcggc
     3301 catcctcacc ggcctggagc gcaacgacgt cctgttcatc gacgagatcc accgcctgtc
     3361 gccggtcgtc gaggaaatcc tgtacccggc gctggaggat ttccagatcg acatcatgat
     3421 cggcgacggc ccggccgcgc gctcgatcaa gctcgacctg cagccgttca cgctcgtggg
     3481 cgccaccacg cgagccggca tgctcaccaa cccgctgcgc gaccgcttcg gcatcgtcgc
     3541 ccgcctcgag ttctacacgt ccgacgaact ggccgcgatc gtcgcgcgct cggccggcct
     3601 gctgagcgtg cccatcgacc ccgacggcgc gctggagctc gcccgccgct cgcgcggcac
     3661 gccccgcatc gccaaccgcc tgctgcgccg cgtgcgcgac tacgccgacg tcaaggcgca
     3721 gggccgcatc acggtgcccg tggccaacgc cgcgctggcg atgctggacg tcgacccgca
     3781 gggcttcgac ctgatggacc gcaagctgct cgaggccatc gtccaccgat tcgacggcgg
     3841 cccggtgggc ctggacaacg tcgccgcagc catcggcgag gagccgggca cgatcgagga
     3901 cgtcatcgaa ccctacctga tccagcaagg ctacctgcag cgcaccccgc gcggccgcat
     3961 cgccaccgtc gccgcctacc gccacctggg cctggcgccg ccggcacgca cgggcaacga
     4021 cctgttcggg atgccgccct aacggaagcc tgcggcgctt caatcggcgc gcgccgccag
     4081 cgccgccgcg gccggcatcc ggtcggccga ttcgtccagc gtcggcgcgt tcaagtccag
     4141 gtggctcgtg tcgttccagc ccaccatcgt caagcccgac gacgtgtgca gcagccggtt
     4201 gatcgccgcg ttgtcgagct gccacaggcg cggggcatcg agcggcacgc gggtcgccgc
     4261 gcggtacagg cagtccagca cgccgccgtg cgccaccagc accaccgtct tgccggcgtg
     4321 gcgcatcgcg atgcgcagcg ccgcgtcgac gcagcgggcg tagaaaacgg tcagcgattc
     4381 gccgtcgccc gtcacgcccg gcgcgaactc ggggtcgcgg ctcttccagc gcgcggcgag
     4441 cgccggccac tgttcggcga cctcggtgtg ggtcatgccc tggaaactgc cgaagtggcg
     4501 ttcgcgcagc ccggtgtcga gctccagcgg gatgcggttg aagctggcga tcgcgtgggc
     4561 cgtcgcctgc gcgcgcgcga ggtcggagct cacgacgatg tcgggcgcgt cgtcggccag
     4621 ccagtgcgcc gtgcgcagtg cctgctcacg gccacggtcg ttcagcggga tgtccagctg
     4681 gccctgcagg cgagcctcga cgttccaggc ggtctcgccg tgccgtaccg cgatcagtcg
     4741 cgtcgcttct ccatggctca tgccgtgctc gttccttgaa ttcaggtgcc ccaggtgatg
     4801 ggctctttct cggccatggc gcgcttcatc atctcgacca tcggccacac gcgctggcgc
     4861 aggctgacgc ggtcgcccgt gccaccggcg tcgtcgtcgt cgccggccgc gcgcggcgat
     4921 tccgccgcga ccgccgattc cagcgcgccg atggccgccg gcatctgccc gacctcgatg
     4981 atgccctgcg gcgtgcgctc cttgccgatc aaccccagca cgcgctcgcc caccggctgc
     5041 gtcatgatga ggtcgcccgt ggccctgctc ttgaacttgt agatcatgtc gggtgtccgg
     5101 ttgaaaaaaa agcggccaag gaaatccctg gccgcgattg tccgcgatcc gcccggcggc
     5161 tcgcctgaac caccgggcga ttcgagggga tgtcagcgat tgcggcggat gcgcaccact
     5221 tcgaccagcg cgatgacggc ggcgccgacg gccgcggcga tcagcagcga cttgatcggc
     5281 tcgtccttca cgtagccgcg cgtcgattcg acggcctgcg cgccgcgctc acgcagggcg
     5341 gccgcgcgct cgcgggcgct gtcgtagccg tcgtgcgcca gctcgcgggc gcgctgcgtc
     5401 gcgcgggaga acagcggctt ggcatcgccg taggccgatt gcaggccgtc g
//
LOCUS       DATCRO010000932        18929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1011924, whole genome shotgun sequence.
ACCESSION   DATCRO010000932 DATCRO010000000
VERSION     DATCRO010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011924"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="ssuC"
                     /locus_tag="VIN75_25385"
     CDS             complement(<1..724)
                     /gene="ssuC"
                     /locus_tag="VIN75_25385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HEY8977572.1"
                     /translation="MRVGALTWRAFAPWIVPLVILLAWEASSRSGWLSVRILPEPLAV
                     ATAFWGLLTSGTLVHDVAISTGRALGGLAIGGGLGLVLGLLTGTFRTAETLLDTTLQM
                     IRNVPALALIPLVILWFGIDETAKVFLVAIGVFFPIYLNTYHGIRAVDAGLVEMARSY
                     GLSGWSLYRQVILPGALPSILVGLRFALGLMWVLLIVSETISAQSGIGYMTMNAREFM
                     QTDVLVLGIIIYALLGKLADSAA"
     gene            complement(733..1914)
                     /gene="ssuD"
                     /locus_tag="VIN75_25390"
     CDS             complement(733..1914)
                     /gene="ssuD"
                     /locus_tag="VIN75_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEY8977573.1"
                     /translation="MKILWFIPTHGDSRYLGTSRGARQTDLDYFKQVAIAADTLGYEG
                     VLLPTGRSCEDSWMVAASLVDVTQRLKFLVALRPGIIQPVQTARMAATLDRLSGGRVL
                     VNLVTGGDPDELAGDGLHLSHEERYELSTEFVSIWRDVLRASHTNEAVDFDGKHLKVK
                     GAKVIYPPVTQPYPPLFFGGSSGPAHDLAASQCDTYLTWGEPPAAVAEKVRDVGSRAE
                     RLGRKLEFGIRLHVIVRETEEEAWAAAGKLISHLDDDIVAAAQKKFAAMDSVGQRRMA
                     ALHGGRFDKANPRAGLEVAPNLWAGVGLVRGGAGTALVGNPEQVAARIQEYADLGMNH
                     FILSGYPHLEEAYRFAELVFPLLPLATREKLVQPTLTGPFGEIVANDIRPKADLARAA
                     S"
     gene            complement(1937..2896)
                     /locus_tag="VIN75_25395"
     CDS             complement(1937..2896)
                     /locus_tag="VIN75_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8977574.1"
                     /translation="MKTGLDRRQAIALLAAGTAGLPAARALAATSELRIGYQKSSVNL
                     MVTRERRLLDATLPGVSVKWVEFPAGPQILEALAVGSLDFGFTGDTPPVFAQAAGKDI
                     WYVGLEPPKPASSAILVPPDSTIRSLGDLKGKRIGFQKGSSSHYLVLRALEKGGLQWS
                     DISPLYLAPSDARAAFEKGALDAWGIWDPYYAAAEIDGRARVLTTGQGLSSNNSFYLA
                     SHELTRQPALVKGLFAALSQADAWGKASLPQTAKFLSDATGVPLAGTIRFLQRRTSGP
                     VTVLKPSDIADQQRVADAFSRIGLIPKPLKVADAVWQPGSSFA"
     gene            complement(2905..3924)
                     /locus_tag="VIN75_25400"
     CDS             complement(2905..3924)
                     /locus_tag="VIN75_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8977575.1"
                     /translation="MPVPSPIVTERARRAWLGRAAAFLLLASGAIGLASGPTSALAEP
                     AKELRIGFQKSASLLVLQKAQGTLEKRLAPQGVSVKWVEFPAGPQLLEGLNVGAVDVG
                     YVGEAPPIFAQAAGARFVYVGNDPAAPATEALVVPKGSPLRSVSELKGKRIALNKGSN
                     VHYLLVKLLEKNGLKYSDVTTVFLPPADARAAFEKGAVDAWVIWDPFLAAAEHQLGAR
                     QLADGTGLVNSFSFYLAQTDFAQANPQVIRTLFANTDEAARFVQADVPRAAALIAPLQ
                     GLDVAVVEASLRRYRYGVAPLTPAVAEQQQKIADTFFELKLIPKAVRIADALPSSGTA
                     TVAGK"
     gene            complement(3963..4526)
                     /gene="ssuE"
                     /locus_tag="VIN75_25405"
     CDS             complement(3963..4526)
                     /gene="ssuE"
                     /locus_tag="VIN75_25405"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8977576.1"
                     /translation="MSVVALAGSPSVPSRSTALLRHVLSRLDDDVPRCEIALRDLPPA
                     ALVRAEFDDPAIRRAREQVAAARVVVIATPIYKASFSGLLKSFLDLLPQDALRGKSVL
                     ALGTGGSAAHLLALDYALRPVLAALGARHILDSVYAVDAQFAPHAIHGHVAHDDIVRR
                     IERALADAPVALRTPVGDAQRSVALAA"
     gene            4877..5401
                     /locus_tag="VIN75_25410"
     CDS             4877..5401
                     /locus_tag="VIN75_25410"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8977577.1"
                     /translation="MKITLHALALAGLLASLVAHAQAPAASAPQTPPAPPARGIPADL
                     ALEAAKTALDTCKGLDQKVGVSVLDSAGELRLLLSSDGAPNRGVQNGALKAKAALAFE
                     IPSGQVAERAKADTAIAAKLAANPTWLARAGAVPVTVGGTLIGAIGVGGARGSEKDEA
                     CAVAGLEKIRGRLY"
     gene            5415..6779
                     /locus_tag="VIN75_25415"
     CDS             5415..6779
                     /locus_tag="VIN75_25415"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEY8977578.1"
                     /translation="MNTATRLRHLAAGIALACTAGAHAGPSVYPEGTTRLDPSRAWPS
                     YVLFTGGDETARLVDLDGKTVHTWRDVGGFAPLFIDPALVGGERGRVLVTLENSDGRG
                     VDLVPGRAGPRVSKTVGELDWNGQLKWQFGPKAPGGLAQQHHDWARLPNGNTLVLANL
                     RHPVPGFKQPEVLDDVAYEVDASGAIVWRWVASEHLEEFGFSAATLALIRASDNADYL
                     HLNNLKPVGPNHWFDAGDKRFAPDNLILHSRNANVIAIVDRHSGHIVWTLGPDYPAIP
                     RGAAARRVPRPVDQISGQHDAQIIPAGLPGAGNLLVFDNQGEAGYPPVELPTTGGSRV
                     LEIDPVTRQVVWQYTAEDSGQPSWAFRSTHISAARRLPNGNTFIDEGQSGRLFQVTRE
                     GDIVWEYVNPYPRIGKDPGTGKATVNHQLYRAQPVPLDWAPAGTPHTETALRTGRPGE
                     GAAR"
     gene            complement(6842..8776)
                     /locus_tag="VIN75_25420"
     CDS             complement(6842..8776)
                     /locus_tag="VIN75_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HEY8977579.1"
                     /translation="MASFDHGSDAGALAAGLPDVATLARLAGELFSALPGAPSAGGVP
                     VPSNPLPAGLSVPPGALGPSTPSAVPHGVLPPGANLVPASPQALANTGASAPALLPHA
                     AVPALGLNDTALQAAPGYDGRAGGVALGVPRTLATPGAFAEPPRAASAQAPLSTNAAP
                     HYFLADRAGDSADAHAAQSIVLPSLGAVHERGFGLPDSDRLRAMLAANQPSPGARAPS
                     APQFYFHDPDSAPVAASDRHPPFDVNAVRRDFPILRERVNGRPLVWFDNAATTQKPQV
                     VIDRLKRFYETENSNIHRAAHELAARATDAYEGARETVRRFIGAGSTDEIVFVRGTTE
                     AINLVAKSWGAQNVAAGDEIVVSHLEHHANIVPWQQLAAAKGATLRVIPVDDDGQVLL
                     EEYRKLLNDRTRIVAVTQVSNALGTVVPVREIVATARRAGICTLVDGAQSVSHMRVDV
                     RELDTDFFVFSGHKIFGPTGIGALYGRKALLDAMPPWQGGGNMIADVTFEKTIYHGPP
                     ARFEAGTGNIADAVGLGAALEYVERLGIENIGRYEHALLEYATHRMAPIPGLRLVGTA
                     RDKASVLSFVLDGYSTQEVGLALNEEGIAVRTGHHCAQPILRRFGLEATVRPSLAFYN
                     TCEEVDRMVDVVRRLARRRG"
     gene            complement(8785..9714)
                     /locus_tag="VIN75_25425"
     CDS             complement(8785..9714)
                     /locus_tag="VIN75_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEY8977580.1"
                     /translation="MVDTTNAPMALGDNAARQLANATKTAPTLSTISPRWLTHLLQWI
                     PVEAGIYRLNTVKNPQDIQVLCAKRDEAELPTTFVDYDEAPREYFLNAVSTIVDVHTR
                     VSDLYSSPHDQIKEQLRLAIETIKEKQESELINHPDYGLLASVAPEQIVYPLTGAPTP
                     DDLDELLTKVWKEPAFFLAHPLAIAAFGRECTRRGVPPPTVSLFGSQFLTWRGIPLVP
                     SDKVPVADGKSKILLLRVGEKRQGVVGLFQPGLAGEQGPGLSVRFMGINRNAIASYLI
                     SLYCSLAVHTADALAVLEDVEIGKYHDYPDTYK"
     gene            complement(9837..10103)
                     /locus_tag="VIN75_25430"
     CDS             complement(9837..10103)
                     /locus_tag="VIN75_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8977581.1"
                     /translation="MDDIDLVGAPLATRRPSARFGAVIRELRESRGWSQEQLAGQAGL
                     NRSYMGEIERSAAMPSLASAEKLAQALGVALSALISHCERAAAR"
     gene            complement(10109..11083)
                     /gene="epsC"
                     /locus_tag="VIN75_25435"
     CDS             complement(10109..11083)
                     /gene="epsC"
                     /locus_tag="VIN75_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY8977582.1"
                     /translation="MAIDLDLPAEAPRADKRHEPALELASIVQQLREVRESWRRQQGR
                     PRELSGRELPSREALAAIVGGLRGALFPMRLGPPDLRQESEDYFVGHTLDAALHSLLA
                     QVRLELRHHSRAAHSDAATLDEQALRVVREFGASLPRIRELLDTDVLAAWRGDPAARS
                     VDEVLVCYPGLHAMIHHRIAHRLHGLGVPLLARIVAELAHGDTGIDIHPGARIGSHFF
                     IDHGTGVVIGETAVIGDNVRIYQAVTLGAKRFEADADGALRKGGARHPIVEDDVVIYA
                     GATVLGRVTIGRGASIGGNVWLTRSVPPGSRIAQAIARDDHVDPGVGG"
     gene            complement(11090..11557)
                     /locus_tag="VIN75_25440"
     CDS             complement(11090..11557)
                     /locus_tag="VIN75_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8977583.1"
                     /translation="MSDPVADAAEDVLLERAINEARAQGLPFAGSVPPSEAWALVESG
                     RAVLVDVRTSDERKFVGYVPESIHVAWASGTSLTRNPRFLRELEAKVDKSDVVLLLCR
                     SGKRSALAAEAATKAGFTAAFNVSEGFEGDLDADGHRGRLGGWRLHGLKWVQD"
     gene            complement(11716..12087)
                     /locus_tag="VIN75_25445"
     CDS             complement(11716..12087)
                     /locus_tag="VIN75_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977584.1"
                     /translation="MIKTVFAAMTLALAASAAVAASAPAAISNGVLATPGGATLYTFD
                     KDVVGNGKSACNNQCATNWPPLAAQASDSGFGDWSVVTRDDGSKQWAYKGAPLYTYAK
                     DAKAGDTTGDGKGNVWHVIKG"
     gene            12916..14637
                     /locus_tag="VIN75_25450"
     CDS             12916..14637
                     /locus_tag="VIN75_25450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8977585.1"
                     /translation="MQLRRLFLVLSCALSVTVVAVTASVVGKAWRDLRQARAGLDEMQ
                     QLQALFIVAEMASRERGPANGVLGDDLPGDPAKRERLLLARKKTDAAFAALQQVLDPA
                     DSRSARVGLTVQAARRQLVRGRQAVDAEAAKDRASRRPAEIRKAVDQMIEVIDDLTPA
                     TIRLTNGASATFPVATNSLVAARQAAALREYAGRLGSLFTGSLTLRQRLTTDERAAID
                     QMRGRIEQLRQQLTTFAAAAEAHQNAQGAIDDMQARYFGSAIPFVDAQIAIGMRDGQY
                     DVDTAQFAARYVPDMDSIIRLRDVLMGDAMEDARHGLAHARDAAMLTGVGAMSTLLLL
                     GLTLWLLHRRVIRPLARTTELIVTIAQGNLDIQVPAPRHHDEVADMLGAIAVLRDNSV
                     ARRTAEEAIRQMAYYDRLTGLPNRRLLEDRMERVLATARRQGRKASVLFIDLDKFKHV
                     NDQRGHEVGDWLLQQVALRMRSVLRESDTAARVGGDEFVVLLPDSTRADDAVHVAEKI
                     RQAIERPFTRDDGVTLEISSSVGVALYPDHAQEARDLLRFGDQAMYVAKKHGRNAVEV
                     FGAGDKA"
     gene            complement(14732..15271)
                     /locus_tag="VIN75_25455"
     CDS             complement(14732..15271)
                     /locus_tag="VIN75_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977586.1"
                     /translation="MPLQDHVRAFADNDAPYMYDGGRQISFGPEMRVGMACHIASFIW
                     LYRAAHAGAFPPLTAFENNAPQHFVDGLIRFGNATRVTMGHGPLPNDVLIFANPDDPY
                     NALHSCVVVANGQRIAGYNQSGWFDNGGVHAYSDHAVDSCDWVARCCCWSTPRRATAA
                     GGQRYVWAVRGATAIGRMA"
     gene            15415..15936
                     /locus_tag="VIN75_25460"
     CDS             15415..15936
                     /locus_tag="VIN75_25460"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8977587.1"
                     /translation="MTPIRLLAAAAVAASMLAAPAQAATWHWDYSATGISAAGTFTTT
                     DAPDADGFYTITAITGSRNGVAITGLEAAGSAIPGNDPYAVDNLVSTGGPQLTWNGFG
                     FAMADGTWANPFSDGSTAWEYLSVPPYPEGAGQEVAVRFSAAPVPEPASGVLALLGVA
                     GLAAAGRRARRVR"
     gene            15965..16315
                     /locus_tag="VIN75_25465"
     CDS             15965..16315
                     /locus_tag="VIN75_25465"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY8977588.1"
                     /translation="MTTISENSGLVTAINVFTVEPSNQQRLVDLLVRATETSARDAPG
                     FVSAALHRSVDGTRVTMYAQWESAEHYARYQAMRSSAGASSYVAQILAIATFAPGTYE
                     VAKVFAGPSKTPGD"
     gene            16484..18019
                     /locus_tag="VIN75_25470"
     CDS             16484..18019
                     /locus_tag="VIN75_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY8977589.1"
                     /translation="MNQQPQAAGRMPRQIPYIIANEGCERFSFYGMRNILTVFLMTSL
                     LLSIPEPLRKNEAKEVFHTFVIGVYFFPLLGGWIADRWFGKYPTILWLSLVYCAGHAC
                     LALFDTNLHGFYFGLFLIALGSGGIKPLVSAFVGDQFDKSNRTLAQKVFEAFYWIINL
                     GSFFASLFMPLILKQFGGAVAFGIPGVFMFIATVIFWSARNRYVHVAPAAHEDPNSFL
                     KVVRTALGLGAGGRAVALVGVALACVALCFWNDLGVVAALCTALVLVLGFGGAGAWMA
                     LDRAEPRHGAEAVAAVRAVLRLLVLFGLVTPFWSLFDQKASTWVLQADQMAKPAWFQS
                     SMMQALNPALVLILIPFNKVVLYPMLESLGLKLTALRRMGAGIAFAGVAWIVVGLMQL
                     WLDGGQKVDITWQVLPYALLTLGEVLVSTTGLEFAYSQAPMSMKGVIMSFWSLSVTVG
                     NLWVLLSNVSVRSDAVNARIAGTGLSVTAFQMFFFAAFALLAALAFALYARRYQERDY
                     YRG"
     gene            18198..>18929
                     /locus_tag="VIN75_25475"
     CDS             18198..>18929
                     /locus_tag="VIN75_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY8977590.1"
                     /translation="MLLSVDGPIGAASADYVERSLHRAERGGAGLVVLQLDTPGGLDT
                     SMRAIVKAILASKVPVAAFVGPSGARAASAGTFMVYASHVAAMAPATELGAATPITMG
                     AAPDPYPSASAGAASSPGVPHDALEAKRINDAAAGIRSLALVRHRNADWAERAVRDAA
                     SLSSTEALSQHVVDLVARDVPDLLRQLDGRAVQLADGRSMTLATAGARIERWEPDWRD
                     RLLAFIGDPSLALMLLLVGFYGLLFE"
BASE COUNT         2801 a         6705 c         6645 g         2778 t
ORIGIN      
        1 gcgcggcgct gtcggcgagc ttgcccagca gggcatagat gatgatgccg agcaccaaca
       61 cgtcggtttg catgaactcg cgcgcgttca tcgtcatgta gccgatgccc gactgcgcgg
      121 agatcgtctc cgacacgatc agcagcaccc acatcagccc gagcgcgaag cgcagcccca
      181 ccaggatgga gggcagcgcg ccgggcagga tcacctgtct gtacaggctc cagcccgaga
      241 ggccgtagct gcgcgccatc tccaccaggc cggcgtcgac cgcgcggata ccgtgatagg
      301 tattgaggta gatcgggaag aacacgccga tcgccaccag gaacaccttg gccgtctcgt
      361 cgatcccgaa ccacaggatc acgagcggga tgagggcgag cgcggggacg ttgcggatca
      421 tctgcagcgt ggtgtccagc agcgtctcgg cggtgcggaa cgtgcctgtc agcaatccca
      481 gcaccaggcc gaggccgccg ccgatcgcca agccgcccag cgcgcggccg gtgctgatgg
      541 cgacgtcgtg caccagcgtt ccggaggtca gcaggcccca gaacgcggtg gccaccgcca
      601 gcggctcggg caggatgcgc accgacagcc agccgctgcg tgacgaggcc tcccacgcga
      661 gcaggatgac cagcgggacg atccacggcg cgaacgcgcg ccaggtcaat gcgccaactc
      721 gcatcgtcga cctcagctgg cggcccgggc caggtcggcc ttcggacgga tatcgttcgc
      781 gacgatctcg ccgaacggcc cggtgagcgt cggctgcacc agcttctcgc gcgtggccag
      841 cggcagcagc gggaacacca gctccgcgaa gcgataggct tcctccaggt gcggatagcc
      901 cgagaggatg aaatggttca tgccgagatc cgcgtattcc tggatgcgtg cggcgacctg
      961 ctcgggattg cccaccaggg ccgtgccggc gccgccccgc acgaggccga cgccggccca
     1021 caggttggga gccacctcga gtccggcgcg cgggttggcc ttgtcgaacc tgccgccgtg
     1081 cagggcagcc atgcgccgct gcccgacgga gtccatggcg gcgaacttct tctgcgccgc
     1141 agcgacgatg tcgtcatcca gatgcgagat cagcttgcct gcggcggccc acgcctcttc
     1201 ctcggtctcg cgcacgatca cgtgcaggcg gatgccgaac tcgagcttgc ggcccaggcg
     1261 ctcggcgcgg ctgcccacgt cgcgcacctt ctcggcgacg gcggccggcg gctcgcccca
     1321 ggtgaggtag gtgtcgcact gcgacgccgc cagatcgtgt gccgggcccg acgacccgcc
     1381 gaagaacagc ggcggatagg gctgcgtgac cggcggatag atgaccttgg cgcccttgac
     1441 cttcaggtgc ttgccgtcga agtcgaccgc ctcgttcgtg tggctggccc gcagcacgtc
     1501 gcgccagatc gacacgaact ccgtcgacag ctcgtagcgc tcctcgtggc tcaggtgcag
     1561 gccgtcgccg gcgagctcgt cggggtcgcc gccggtgacg aggttgacca gcacgcggcc
     1621 gcccgacagc cggtccagcg tggccgccat gcgcgccgtc tgcaccggct ggatgatgcc
     1681 cggacgcagc gcgaccagga acttcagccg ctgcgtgaca tcgaccaggc tggccgcgac
     1741 catccacgag tcctcgcacg agcggcccgt gggcagcaga acgccttcgt agccgagcgt
     1801 gtcggcggcg atcgccacct gcttgaagta gtcgaggtcg gtctggcgcg cgcccctgga
     1861 ggttccgagg tagcgcgagt cgccgtgcgt ggggatgaac cagaggatct tcatgacggg
     1921 gtctttcgag cggaggtcag gcgaaggacg agccgggctg ccacacggcg tcggcaacct
     1981 tcagcggctt ggggatgagg ccgatgcggc tgaacgcgtc ggcgacgcgt tgctggtccg
     2041 cgatgtcgga cggcttgagc accgtcacgg ggccggatgt gcggcgctgc aggaagcgga
     2101 tcgtcccggc cagaggcacg ccggtcgcgt ccgacaggaa cttcgccgtc tgcggcaggc
     2161 tggccttgcc ccaggcgtcg gcctgtgaca gcgcggcgaa caggcccttg accagcgccg
     2221 gctgccgcgt cagctcgtgc gaggcgaggt agaagctgtt gttgctggac aggccctggc
     2281 cggtcgtgag cacacgcgcc cggccgtcga tctcggcggc cgcgtagtac gggtcccaga
     2341 tgccccaggc gtccagcgcg cccttctcga atgccgcgcg cgcatccgac ggcgccaggt
     2401 acagcggcga gatgtccgac cactggaggc cgcccttctc gagcgcgcgc agcaccagat
     2461 agtgcgaact cgagcctttc tggaagccga tgcgcttgcc cttgagatcg ccgagcgagc
     2521 gaatggtcga atcgggcggc accaggatcg cggagctggc cggcttcggc ggctcgaggc
     2581 cgacgtacca gatgtccttg ccggccgcct gcgcgaacac gggcggcgtg tcgccggtga
     2641 agccgaagtc gaggctgccc acggccaggg cctcgaggat ctgcgggccg gccggaaact
     2701 cgacccactt cacgctcacg ccgggcagcg tcgcatcgag caggcggcgc tcgcgggtga
     2761 ccatcaggtt gaccgaggac ttctggtagc cgatgcgcaa ctccgatgtt gccgcgaggg
     2821 cgcgagcggc cggcaggccc gcggtcccgg cggccagcag cgcgatcgcc tgacggcggt
     2881 caaggcctgt cttcatgcgg cccctcactt gcccgcgacc gtcgcggtgc cgctggacgg
     2941 cagtgcgtcc gcgatgcgca cggccttcgg gatcagcttg agctcgaaga aggtgtcggc
     3001 gatcttctgc tgctgctcgg ccacggcggg cgtgagcggc gcgacgccgt agcggtagcg
     3061 cctcaggctc gcttccacga cggccacgtc cagcccttgc agcggcgcga tcaatgccgc
     3121 ggcgcgaggc acgtcggcct gcacgaagcg cgcggcctcg tcggtgttgg cgaacagcgt
     3181 gcggatcacc tgcgggttgg cctgcgcgaa gtccgtctgc gccaggtaga agctgaagct
     3241 gttgaccagg cccgtgccat ccgcgagctg gcgggcgccg agctggtgct ccgccgcggc
     3301 caggaacgga tcccagatga cccaggcgtc gaccgcgccc ttctcgaacg cggcgcgcgc
     3361 gtcggcgggc ggcaggaaga cggtcgtgac gtccgaatac ttcaggccgt tcttctccag
     3421 cagcttcacg agcaggtagt gcacgttgga gcccttgttc aatgcgatcc gcttgccctt
     3481 gagttcgctc acggacctga gcggcgaccc cttgggcacg accagcgcct cggtcgccgg
     3541 ggcggccgga tcgttgccga catagacgaa gcgcgcgccg gcagcctgcg cgaagatcgg
     3601 cggcgcctcg cccacgtagc cgacgtcgac ggcccccacg ttcaggcctt cgagcagctg
     3661 cgggccggcg ggaaactcca cccacttcac cgagacgccc tgcggcgcca ggcgcttctc
     3721 cagcgtgccc tgcgccttct gcagcacgag caggctggcc gacttctgga agccgatgcg
     3781 cagctccttg gcgggttcgg ccagcgccga cgtggggccc gatgcgaggc cgatcgcgcc
     3841 tgacgccagc aacaggaacg cagctgcgcg gccgagccag gcgcggcggg cgcgctccgt
     3901 gacgatgggc gatggaacgg gcatgtggat cctccggaag tttcgttcga gatgtgcggc
     3961 tgtcaggccg cgagcgcgac ggagcgctgc gcgtcgccga caggggtgcg caatgcgacc
     4021 ggcgcatccg cgagcgctcg ctcgatgcgc cggacgatgt cgtcgtgcgc gacgtgcccg
     4081 tgaatcgcgt gcggcgcgaa ctgcgcatcg acggcataca cggaatccag gatgtggcgc
     4141 gcgcccagcg ccgcgagcac gggccttagc gcatagtcga gagccagcag gtgggccgcg
     4201 ctgccgccgg tgcccagcgc cagcaccgac ttgccgcgca acgcgtcctg cggcagcagg
     4261 tcgaggaagg acttgagcag gccgctgaag gaggccttgt agatcggcgt ggcgatcacc
     4321 accacccggg ctgccgcgac ctgctcgcgg gcgcggcgga tcgccgggtc gtcgaactcg
     4381 gcccgcacga gcgccgccgg cggcaggtcg cgcagcgcga tctcgcagcg cgggacgtcg
     4441 tcgtcgagcc ggctgagcac atggcgcagc aaggcggtgg atcgcgacgg gacggaagga
     4501 ctgccggcaa gggcgacgac ggacatggcg acggtgttga tgattcgaca gtcccccgac
     4561 gttagggtca catcgcctgc acgaaaacga acacaaacgg ctatcgatat ccgtgcggcg
     4621 actttgtcgg tgacagcgcg accttgctcg ccgccgcgtg gatcgaagcg cgtcaaccgg
     4681 tcgcgatgag cacctccacg cgagcgcaca gcggatcgcc ggggcaagca aatccgggat
     4741 cgttccttcg acgggccgct gctcgggcgc gcgtgcgtga cgcccgcgca cgccgcatgg
     4801 cgcagagctt gcattgagaa cgtcgatcgc ctcgttccgg ccgacccggc cggcttccac
     4861 agcactcgac gctcccatga aaatcacgct gcacgccctg gctctcgcgg gcctcctcgc
     4921 ctcccttgtc gcccacgccc aggctccggc cgccagcgcc ccgcagacgc cgcccgcacc
     4981 tcccgcacgc ggcattcccg ccgacctcgc gctcgaggcc gccaagacgg cgctcgatac
     5041 ctgcaagggc ctggaccaga aggtgggtgt cagcgtgctc gattccgcgg gcgagctgcg
     5101 cctgctgctg tcctcggacg gcgcccccaa ccgcggcgtg cagaacggtg cgctcaaggc
     5161 caaggccgcg ctcgccttcg agatccccag cggccaggtg gccgagcgcg cgaaggccga
     5221 caccgccatc gccgcgaaac tcgccgccaa tccgacctgg ctggcccgcg ccggcgcggt
     5281 tcccgtgacg gtcggcggca cgctgatcgg cgccatcggc gtcggcggcg cgcgcggcag
     5341 cgagaaggac gaggcctgcg ccgtcgccgg cctcgagaag atccgcggcc gcctgtactg
     5401 aaccggagcc gacgatgaac accgccacgc gcctgcgcca cctcgccgcc ggcatcgccc
     5461 tcgcctgcac cgccggcgcg cacgccggcc ccagcgtcta cccggagggc acgacgcgcc
     5521 tcgatccctc gcgcgcgtgg ccgtcgtacg tgcttttcac cggcggcgac gagacggccc
     5581 gcctcgtcga cctcgacggc aagaccgtgc acacgtggcg cgacgtcggc ggattcgcgc
     5641 ccttgttcat cgatccggcg ctggtgggcg gcgagcgcgg gcgggtgctg gtcacgctgg
     5701 agaacagcga tggccgcggc gtggacctcg tgcccggacg cgcgggtccg cgcgtctcga
     5761 agaccgtggg cgaactcgat tggaacgggc agctgaagtg gcagttcgga ccgaaggcgc
     5821 cgggcggcct ggcgcaacag caccacgact gggcacgcct gccgaacggc aacacgctgg
     5881 tgctcgcgaa cctgcgccat cccgtgcccg gtttcaagca gcccgaggtg ctggacgacg
     5941 tggcctacga ggtcgatgcg agcggcgcga tcgtctggcg ctgggtggcg tccgagcatc
     6001 tcgaggagtt cggcttcagc gccgccacgc tggcgttgat ccgcgcctcc gacaacgccg
     6061 actacctgca cctgaacaac ctcaagccgg tcggccccaa ccactggttc gacgctggcg
     6121 acaagcgctt cgcaccggac aacctgatcc tccattcgcg caacgccaac gtgatcgcca
     6181 tcgtcgaccg ccacagcggc cacatcgtgt ggacgctcgg ccccgactac cccgcgatcc
     6241 cgcgcggcgc cgcggctcgg cgcgtgccgc gtcccgtcga ccagatcagc ggccagcacg
     6301 acgcgcagat catccccgcc ggcctgcccg gcgcgggcaa cctgctggtg ttcgacaacc
     6361 agggcgaggc cggctacccg cccgtcgaac tgcccaccac cggcggctcg cgcgtgctgg
     6421 agatcgaccc ggtcacgcgg caggtcgtct ggcagtacac ggcggaggac tcgggccagc
     6481 catcgtgggc gttccgcagc acgcacatca gcgccgcgcg ccgcctgccc aacggcaaca
     6541 ccttcatcga cgaaggccag agcggtcggc tgttccaggt gacgcgcgag ggcgacatcg
     6601 tgtgggaata cgtcaatccg tatccgcgca tcggcaagga tcccggcacc ggcaaggcca
     6661 cggtcaacca ccagctctat cgcgcgcagc cggtgccact ggactgggcc cctgcgggca
     6721 cgccgcatac cgagacggca ctgcgcacgg gccgacccgg cgagggcgct gcgcgatgag
     6781 tgtcccggcg gtgttcatcg gccggtcgat gcggacggca cgaccctttc ggggtgaggt
     6841 ctcagccgcg ccggcgcgcc agccggcgca cgacatccac catgcggtcg acttcctcgc
     6901 aggtgttgta gaacgcgagc gacggccgca cggtcgcttc caggccgaag cgccgcagga
     6961 tcggctgcgc gcagtggtgg cccgtgcgca ccgcgatccc ctcctcgttg agcgccaggc
     7021 ccacctcttg cgtgctgtag ccgtccagca cgaaggacag gacgctggcc ttgtcgcgcg
     7081 cggtgcccac caggcgcagg cccgggatgg gcgccatgcg gtgcgtggcg tattcgagca
     7141 gcgcgtgctc gtagcggccg atgttctcga tgcccaggcg ttcgacgtat tccagcgcgg
     7201 cgcccaggcc cacggcgtcc gcgatgttgc cggtgcccgc ctcgaatcgc gccggcggtc
     7261 cgtggtagat cgtcttctcg aacgtgacgt cggcgatcat gttgccgccg ccctgccacg
     7321 gcggcatcgc gtcgagcagg gccttgcggc catacagcgc accgatgcct gtcgggccga
     7381 agatcttgtg cccggagaac acgaagaaat cggtgtcgag ctcgcgcacg tccacgcgca
     7441 tgtgcgagac cgactgcgcg ccgtcgacca gcgtgcagat gcccgcgcgt cgcgcggtcg
     7501 cgacgatctc gcgcaccggc acgacggtgc ccagcgcgtt ggacacctgc gtcaccgcga
     7561 cgatcctggt gcggtcgttg agcagcttgc ggtactcttc cagcagcacc tggccgtcgt
     7621 cgtcgaccgg gatcacgcgc agcgtcgcgc ccttggccgc ggccagttgc tgccatggca
     7681 cgatgttggc gtggtgctcg aggtgcgaga cgacgatctc gtcaccggca gccacgttct
     7741 gcgcgcccca gctcttggcc accaggttga tcgcctcggt ggtgccgcgc acgaacacga
     7801 tctcgtcggt cgagccggcg ccgatgaagc gccgcaccgt ctcgcgcgcg ccctcgtagg
     7861 cgtcggtcgc gcgcgccgcc agctcgtgcg ccgcgcggtg gatgttggag ttctcggtct
     7921 cgtagaagcg cttcaggcga tcgatgacga cctgcggctt ctgcgtggtg gccgcgttgt
     7981 cgaaccagac cagcggcctg ccgttgacgc gttcgcgcag gatggggaag tcgcgccgca
     8041 cggcattgac gtcgaacggt ggatggcgat cgcttgcggc gacaggcgcc gaatccggat
     8101 cgtggaaata gaactgcggc gccgacggcg cgcgtgcgcc gggcgagggc tggttggcgg
     8161 ccagcatggc gcgcaggcga tcgctgtccg gaaggccgaa gcctcgctcg tggaccgcgc
     8221 cgagcgacgg cagcacgatc gactgcgcgg catgcgcgtc ggccgaatcg cccgcgcgat
     8281 ccgcgaggaa gtaatgcggc gcagcgttgg tggacagcgg cgcctgcgcg gacgccgcgc
     8341 gcggcggctc cgcgaacgcg cccggcgtcg cgagcgtgcg cggtacgccc agcgcgacgc
     8401 cgcccgcacg accgtcgtag cccggcgcgg cctgcaacgc ggtgtcgttc agtcccagcg
     8461 caggcacggc cgcgtgcggc agaagcgccg gtgcggacgc gcccgtgttg gccagcgcct
     8521 gcggcgacgc gggaacgagg ttcgcacccg gcggcagcac gccgtgcggc accgccgacg
     8581 gcgtggacgg cccgagcgcg ccgggcggca cgctcagtcc cgcgggcagg ggattggacg
     8641 gcaccggcac gccgccggcc gacggcgcgc cgggcaacgc gctgaagagc tcgccggcga
     8701 gccgggcgag cgtcgcgacg tccggcagtc cggcggcgag cgcgccggcg tcgctgccgt
     8761 gatcaaagga ggccatcgcg cggcttactt gtaggtgtcg ggatagtcgt ggtacttgcc
     8821 gatctcgacg tcctcgagca cggccagcgc gtcggccgtg tgcacggcga gcgagcagta
     8881 cagggagatc aggtaggacg cgatcgcgtt gcggttgatg cccatgaagc gaaccgacag
     8941 ccccggcccc tgctcgccag ccaggcccgg ttggaacaga cccaccacgc cctggcgctt
     9001 ctcgccgacg cgcagcagca ggatcttgct cttgccgtcg gccaccggca ccttgtcgga
     9061 cggcaccagc gggatgccgc gccaggtcag gaattgcgag ccgaacaggc tcaccgtcgg
     9121 cggcggcacg ccgcggcgcg tgcactcgcg gccgaacgcc gcgatcgcca gcggatgcgc
     9181 gaggaagaac gccggctcct tccacacctt cgtcagcaac tcgtcgaggt cgtccggcgt
     9241 gggcgcgccg gtcagcgggt agacgatctg ctccggcgcg acgctggcca gcaggccgta
     9301 gtccggatgg ttgatcagct cgctctcctg cttttccttg atcgtctcga tcgccaggcg
     9361 cagctgttcc ttgatctggt catgcgggct gctgtagagg tcggacacgc gcgtgtgcac
     9421 gtccacgatg gtgctgaccg cgttcaggaa gtattcgcgc ggcgcttcgt cgtagtcgac
     9481 gaaggtggtg ggcagttcgg cctcgtcgcg cttggcgcac agcacctgga tgtcctgcgg
     9541 gttcttgacc gtgttgaggc ggtagatgcc ggcctcgacc gggatccact gcagcagatg
     9601 cgtcagccag cgcgggctga tggtggacag cgtcggcgcg gtcttggtgg cgttcgcgag
     9661 ctggcgggcg gcgttgtcgc cgagcgccat cggcgcgttc gtggtgtcga ccatgtgcag
     9721 ggagtcctcg tgctgaaagc gaatttgaat atgcgaaatg cgctgaagaa agtatcccca
     9781 ccgctgtcga gcggatcaac cggctatagc caccaaagcg gcctggacgg cgcccgtcag
     9841 cgagccgccg cccgctcgca gtgcgagatc agcgccgaca gcgcgacgcc cagcgcctgc
     9901 gcgagcttct ccgcgctggc gagcgacggc atcgcggccg agcgctcgat ctcccccatg
     9961 tacgagcggt tcaggcccgc ctggccggcg agctgctctt gcgaccagcc gcgcgactcg
    10021 cgcagctcgc ggatcactgc gccgaagcgc gcggaagggc gccgggtcgc cagcggggcg
    10081 cccacgaggt cgatgtcatc catcgcgctc agccgccgac gcctggatcg acatggtcgt
    10141 cgcgtgcgat cgcctgagcg atgcggctac ccggcggcac gctgcgtgtg agccagacat
    10201 tgccgcctat ggacgcgccg cggccgatgg tgacgcggcc cagcacggtc gccccggcgt
    10261 agatcacgac atcgtcctcc acgatgggat ggcgtgcgcc gcccttgcgc agcgcaccgt
    10321 ctgcgtccgc ctcgaagcgc ttcgcgccca gcgtgacggc ctggtagatg cgcacgttgt
    10381 cgccgatcac cgcggtctcg ccgatcacga cgcccgttcc gtggtcgatg aagaagtgcg
    10441 agccgatgcg ggcgccgggg tggatgtcga tgccggtgtc gccgtgcgcc agctcggcaa
    10501 cgatgcgcgc gagcagcggc acgcccaggc cgtgaaggcg atgggcgatg cggtgatgga
    10561 tcatcgcgtg caggccgggg tagcagacca gcacctcgtc gacgctgcgc gcggccgggt
    10621 cgccgcgcca ggcggccagg acgtcggtgt ccagcaactc gcggatccgc ggcagcgacg
    10681 cgccgaactc gcgcacgacc cgcagcgcct gctcgtcgag cgtcgccgcg tccgagtggg
    10741 cagcgcgcga gtgatggcgc agctccagcc gaacctgcgc gagcagcgaa tgcagcgccg
    10801 cgtccagcgt gtggccgacg aagtagtcct cgctctcctg gcgcaggtcg ggcggcccca
    10861 ggcgcatcgg gaacagcgcg ccgcgcaggc cgccgacgat ggccgccagc gcctcgcgcg
    10921 acggcagctc gcggccgctc agctcgcgcg ggcgaccttg ttgacgtcgc cagctctcgc
    10981 gcacctcgcg caactgctgc acgatgctgg cgagttccag cgcgggttcg tggcgcttgt
    11041 cggcgcgcgg cgcctcggcg ggcaggtcca gatcgatggc catggcgctt cagtcctgca
    11101 cccacttcag gccatgcagg cgccagccgc ccaggcgacc gcgatggccg tccgcgtcga
    11161 ggtcgccctc gaaaccctcg ctgacgttga aggcagccgt gaatcccgcc ttggtggcag
    11221 cctcggccgc gagcgcagag cgcttgccgc tgcggcacag cagcagcacg acgtcgctct
    11281 tgtcgacctt ggcttccagc tcgcgcagga agcgcggatt gcgcgtcagc gacgtgccgg
    11341 aggcccacgc gacgtggatg ctctccggca cgtagccgac gaacttgcgc tcgtcgctcg
    11401 tgcgcacgtc gaccagcacg gcgcgacccg actcgaccag cgcccaggcc tcgctcggcg
    11461 gcacgctgcc cgcgaacggc aggccctgcg cacgggcttc attgatggcg cgttcgagga
    11521 ggacgtcttc ggcggcgtcg gcgacggggt cggacatggg aactccggac attggtggcg
    11581 atagccggca gattagggcg ccatcgcgct cgcgtgaaat gccgaattcg ccgatgcata
    11641 ggcgtccacc gtctctagcc gagcgatgtg cgccttccgt ggctccatgg ccgccgacct
    11701 cgtcccacgg atccgtcagc ccttgatcac gtgccagacg ttgcccttgc cgtcacccgt
    11761 ggtgtcgccg gccttggcgt ccttggcata ggtgtagagc ggcgcgccct tgtaggccca
    11821 ctgcttcgag ccgtcgtcgc gggtgacgac ggaccagtcg ccaaagccac tgtcggacgc
    11881 ctgcgcagcc agcggcggcc agttggtggc gcactggttg ttgcaggcgc tcttgccgtt
    11941 gcccacgacg tccttgtcga aggtgtagag cgtggcgccg ccgggcgtcg cgaggacgcc
    12001 gttcgaaatc gcggccggcg ccgaagcggc gacggcggcg ctggccgcga gtgccagggt
    12061 catggcggca aagacggtct tgatcattgc tgttctccag gtgttttggt gacgccggca
    12121 ccgcgggagc gtggcgccgg ccgccgcagg attgcaagat ccgaaccgcc tccggtccgc
    12181 aggcgagagt ggcggacgcc gagcacgcac actgtcgatg ccgccgcagc actgcctgga
    12241 aacgaacagc ccctgcgccg tttcgcgcac catcgacccg ttcgaggacg ccccaggcgc
    12301 ccgctgatgc gccagcgcga gttcgactgc gagtagtcga tcgagaaacc gcgccatgcg
    12361 tcgatgtcga agacgacaac ctccctgcac aacgacgacc ccaagcgtgg cagggagatg
    12421 gcggccgcct tcggcgcaag accccgggat tgcctgctcc ttgccgatga agccggagaa
    12481 cccatccgcg caaccgacag acgccggcca actccggccg ccatgccgct tgagcgtcat
    12541 acatcacgga tggccgcgcg cggtggtcgc gccgccaccg gatcgccgcg ccggccccta
    12601 tgatgggccg tcctcgacac aggcgcgcgc cacgcgggtc gggtgattcg cgcctgggca
    12661 cgcgcgcctt ccggctcgct gcgagtcgcc gcagatcgcc gcgatcggat cgatagcttc
    12721 ctgcgtgtcg ctgccgccat ccgggcgtcg cgaggtctcg tccacgcctt cgtggacatc
    12781 gctcggcgga aggcagacga tgcggctggc agcgttgcgg ccgcccgcgg cacggggcgg
    12841 gatttccgtc gaaggcgcgg gcgaacacca gccaggcgtc gccgcggcgg ctgcgaatca
    12901 aacgagtatc ggcaagtgca acttcgacgt ctcttccttg tcctgagctg cgcgctgtcc
    12961 gtcaccgtcg tcgccgtgac ggccagcgtc gtcggcaagg catggcggga cctgcgccag
    13021 gcccgggccg gcctcgacga gatgcaacag ttgcaggcct tgttcatcgt cgcggagatg
    13081 gcttcgcgcg aacgcggtcc ggccaacggc gtcctgggcg acgacctgcc cggggatccc
    13141 gccaagcggg agcgcctgct gctcgcgcgc aagaagacgg acgcggcctt cgcggccctg
    13201 cagcaggtgc tggatcctgc cgacagccgc agcgcgcgcg tgggcctgac ggtccaggcc
    13261 gcgcgccggc agctcgtgcg cgggcggcag gcggtcgatg ccgaggccgc gaaggaccgg
    13321 gccagccggc gtccggccga gatccgcaag gcggtcgacc agatgatcga ggtcatcgac
    13381 gacctcacgc cggcgacgat ccggttgacc aacggcgcgt cggccacctt tccggtcgcc
    13441 accaattcgc tggtcgcggc gcgccaggcg gccgcgctgc gcgaatatgc ggggcgcctc
    13501 ggctcgctgt tcaccgggtc gctgacgctc aggcagcgcc tgacgaccga cgagcgcgcg
    13561 gcgatcgacc agatgcgcgg gcgcatcgaa cagcttcgcc agcagctgac gacgttcgcc
    13621 gccgccgccg aggcgcacca gaacgcgcag ggcgccatcg acgacatgca ggcgcgctac
    13681 ttcggcagcg cgatcccgtt cgtcgacgcg cagatcgcca tcggcatgcg cgacggccag
    13741 tacgacgtgg acaccgcgca gttcgccgcg cgctacgtcc ccgacatgga ctcgatcatc
    13801 cggctgcgcg acgtgctgat gggcgacgcg atggaggacg cgcgccacgg cctggcgcat
    13861 gcccgcgacg ccgcgatgct caccggcgtc ggcgccatgt cgacgctcct gctgctgggc
    13921 ctgaccctgt ggctgctgca ccggcgagtg atccggcccc tggccaggac gaccgagctc
    13981 atcgtgacca tcgcccaggg gaacctggac atccaggtgc ccgcgccgcg gcatcacgac
    14041 gaggtggccg acatgctggg cgccatcgcc gtcctgcgcg acaacagcgt cgcccgccgc
    14101 acggcggaag aggcgatccg ccagatggcc tactacgacc gcctgaccgg cctgccgaac
    14161 cggcgcctgc tcgaggatcg gatggagcgg gtgctggcga ccgcgcggcg ccaaggcagg
    14221 aaggcgtccg tcctgttcat cgacctggac aagttcaagc acgtcaacga ccagcgcggc
    14281 cacgaggtcg gcgactggtt gctgcagcag gtggcgctgc gcatgcgatc cgtgctgcgc
    14341 gagtcggaca cggccgcacg cgtgggcggc gacgagttcg tcgtgctcct gcccgattcg
    14401 acccgggccg acgacgccgt gcacgtggcc gagaagatcc gccaggcgat cgagcgcccc
    14461 ttcacgaggg acgacggcgt cacgctggag atctcgtcca gcgtcggcgt ggccctctat
    14521 cccgaccacg ctcaggaggc tcgcgacctg ctgcgcttcg gcgaccaggc gatgtatgtc
    14581 gccaagaagc acgggcgcaa tgcggtcgag gtgttcggag cgggcgacaa ggcctgaata
    14641 ccgcgacacg ccgcgcggcg cgaatgcccg ggagtaacgt ccttccagcg gcgcgtctcc
    14701 atcctcgcgc cgcacgacgc cgccccgcag ctcaggccat gcgcccgatc gccgtcgccc
    14761 cgcgcaccgc ccagacgtag cgctgcccgc cggcggccgt ggcgcgccgc ggcgtgctcc
    14821 agcagcagca gcgcgcgacc cagtcgcagc tgtccacggc gtggtcggaa taggcgtgca
    14881 cgccgccgtt gtcgaaccag cccgactggt tgtagccggc gatgcgctgg ccgttggcga
    14941 cgaccacgca cgaatgcaat gcgttgtacg ggtcgtcggg gttggcgaag atgagcacgt
    15001 cgttgggcag aggcccgtgc cccatggtga cgcgcgtcgc gttgccgaag cggatcaggc
    15061 cgtcgacgaa gtgctgcgga gcgttgttct cgaaggccgt cagcggcggg aaggcgccgg
    15121 catgcgccgc gcggtacaac cagatgaagc tggcgatgtg gcaggccatg cccacgcgca
    15181 tctcggggcc gaacgagatc tggcgtccgc cgtcgtacat gtagggcgcg tcgttgtcgg
    15241 cgaatgcgcg cacgtggtcc tggaggggca tgggtcgtcc tttcgaggcc tgcctgccgg
    15301 caatctagcc gcgggcgtcc cgccgttcaa tcacgccgca tcacgcgcgc ccgcaacagg
    15361 ccgatccgtt cgccgataca gtgacgcttc caacgacaaa cccaaggaga accgatgacc
    15421 ccgatccgat tgctggccgc cgcggcggtc gccgcctcga tgctggccgc gccggcgcag
    15481 gccgccacct ggcactggga ctacagcgcg accggcatct ccgccgccgg caccttcacc
    15541 accaccgacg cgccggacgc ggacggcttc tacacgatca ctgccatcac cggttcgcgc
    15601 aacggcgtcg ccatcacggg actggaggcc gccggcagcg ccatcccggg caacgacccg
    15661 tacgccgtcg acaacctggt ttcgaccggc ggcccgcaac tgacctggaa cggcttcggc
    15721 ttcgcgatgg ccgacggcac ctgggccaat ccgttctcgg acgggtcgac cgcctgggaa
    15781 tacctgtcgg tgccgccgta tccggaaggc gcgggccagg aggtggcggt caggttcagc
    15841 gcggcgccgg tccccgagcc tgccagcggc gtgctcgcgc tgctcggggt ggcgggcctg
    15901 gccgcggcgg ggcggcgcgc ccgacgcgtc cgctgagcgg atcgtcccgc cagcgagacg
    15961 attcatgacg accatttccg agaactccgg cctcgtcacg gcgatcaacg tgttcacggt
    16021 cgagccgtcg aaccagcagc ggctggtcga cctgctcgtg cgcgccacgg agacatcggc
    16081 tcgcgacgcg cccggattcg tctcggcggc gctgcatcgc agcgtcgacg ggacccgggt
    16141 cacgatgtac gcgcagtggg agagcgcgga gcactacgcg cgctaccagg cgatgcgttc
    16201 gagtgctggc gcgtcgtcct acgtggcgca gatcctcgcc atcgccacgt tcgcccccgg
    16261 cacgtacgag gtggccaagg tcttcgcggg gccgtcgaag accccgggcg attgatcgct
    16321 cgcggcggcc ccggcgggcg gcgcagccgg cccgcgccgc cctgcctgcc ctcgccagcc
    16381 gcgcgcggcg caagcagggc agatgcctag gcaaccggcg tgtccggcag cgtaagctac
    16441 ggccccacac cacatccgcc ctccccaccc gaggaaccga ctgatgaacc agcagccaca
    16501 agccgccggc cggatgccgc gccagattcc ctacatcatc gccaacgagg gatgcgagcg
    16561 cttcagcttc tacggcatgc gcaacatcct cacggtgttc ctgatgacga gcctgctgct
    16621 gtcgatcccg gagccgctgc gcaagaacga ggccaaggag gtcttccaca ccttcgtcat
    16681 cggcgtgtac ttcttcccgc tcctgggcgg ctggatcgcc gaccgctggt tcggcaagta
    16741 ccccaccatc ctctggctga gcctggtcta ctgcgccgga catgcctgcc tcgcgctgtt
    16801 cgacacgaac ctgcacggct tctacttcgg cctgttcctg atcgcgctcg gctcgggcgg
    16861 catcaagccg ctggtcagcg ccttcgtcgg cgaccagttc gacaagagca accgcaccct
    16921 ggcgcagaag gtcttcgaag ccttctactg gatcatcaac ctgggcagct tcttcgcgtc
    16981 gctgttcatg ccgctcatcc tcaagcagtt cggcggcgcc gtcgccttcg gcatcccggg
    17041 cgtgttcatg ttcatcgcga cggtcatctt ctggtcggcc cgcaatcgct acgtgcacgt
    17101 ggcgcccgcc gcccacgagg atccgaacag cttcctgaag gtcgtgcgca ccgcgctcgg
    17161 cctgggagcc ggcgggcgcg cggtggccct cgtcggcgtc gcgctggcct gcgtggcgct
    17221 gtgcttctgg aacgatctcg gtgtcgtggc ggcgctgtgc accgcgctcg tcctggtcct
    17281 cggcttcggc ggcgccggcg cctggatggc gctggaccgg gcggagccca ggcacggcgc
    17341 ggaagccgtg gccgccgtgc gcgccgtgct gcgcctgctc gtgctcttcg gcctggtcac
    17401 gccgttctgg tcgctgttcg accagaaggc gtccacctgg gtgctgcagg ccgaccagat
    17461 ggccaagccc gcctggttcc agtcctcgat gatgcaggcg ctgaacccgg cgctggtgct
    17521 gatcctgatc ccgttcaaca aggtcgtgct ctatccgatg ctcgagagcc tcggcctgaa
    17581 gctgaccgcg ctgcgccgca tgggcgccgg catcgcgttc gcgggcgtgg cctggatcgt
    17641 cgtgggcctg atgcagctgt ggctggacgg cggccagaag gtcgacatca cctggcaggt
    17701 gctgccctac gcgctcctga cgctcggcga ggtgctggtc tcgacgaccg gcctggagtt
    17761 cgcctacagc caggcgccca tgtcgatgaa gggcgtgatc atgagcttct ggagcctgtc
    17821 ggtgaccgtc ggcaacctct gggtcctgct gtccaacgtc agcgtgcgca gcgatgccgt
    17881 gaacgcgcgc atcgccggca ccggcctgtc ggtcacggcc ttccagatgt tcttcttcgc
    17941 ggccttcgcg ctgctcgccg cactggcctt cgcgctctac gcgcggcgct accaggagcg
    18001 ggactactac cgcggctgac gccaggggac cctgcgcggc ggcggccgcc gccatggacg
    18061 aatgcgtccg ccgggcctac catcgtgcca tgcgcagcat ggttgctcca ctgtgcgcgc
    18121 tggccctgtg gtccgcgtca cttgccggcg cccagacatc tcccgctgcc gttcccgcgg
    18181 cacgtgggag cgacgtggtg ttgctgagcg tggacggccc gatcggcgcc gccagtgccg
    18241 actacgtgga gcgcagcctg caccgcgcag aacgcggcgg tgccgggctc gtcgtgctgc
    18301 agctcgacac gcccggcggc ctggacacct cgatgcgggc catcgtcaag gcgatcctcg
    18361 catcgaaggt gcccgtggcg gcgttcgtcg gcccgtcagg cgcgcgcgcc gcgagcgccg
    18421 gcacgttcat ggtctatgcg agccacgttg ccgccatggc gccggcgacc gagctcgggg
    18481 cggcgacgcc gatcacgatg ggagcggcgc ccgatccgta cccgtccgcg agcgccggcg
    18541 cagcctcgtc acccggcgtc ccgcacgatg cgctggaagc caagcggatc aacgatgccg
    18601 cagcgggcat ccgctcgctg gcgcttgtgc gtcatcggaa tgcggactgg gccgagcgcg
    18661 ccgtccgcga cgcggcgagc ctgtcgtcga ccgaagccct gtcgcagcac gtcgtcgacc
    18721 tggtggcgcg cgacgttccc gacctgctgc gccagctcga cggccgtgcc gtgcaactgg
    18781 ccgacggccg ctcgatgacg ctggcgaccg cgggcgcccg gatcgagcgc tgggagcccg
    18841 attggcgtga ccggctgctg gccttcatcg gcgacccgag tctcgcgctg atgctgctcc
    18901 tcgtgggctt ctacggcctg ctgttcgag
//
LOCUS       DATCRO010000933         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1068031, whole genome shotgun sequence.
ACCESSION   DATCRO010000933 DATCRO010000000
VERSION     DATCRO010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068031"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VIN75_25480"
     CDS             complement(<1..188)
                     /locus_tag="VIN75_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8977591.1"
                     /translation="MIRHHPDDALLLGHAGGGLATGPALLVEAHLEGCARCRQAVRAF
                     ERVGGELLQAEPPQALAGG"
     gene            complement(185..931)
                     /locus_tag="VIN75_25485"
     CDS             complement(185..931)
                     /locus_tag="VIN75_25485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8977592.1"
                     /translation="MPPDRAGIDDAPGSPQNPIPGLARNHLMDAEHSQQPTPLRPSEP
                     EEGMPHLRLVVSTADDRAPGRDTRLDDWMIAVARHQDRAAFGSLFEHFAPRLKGWLWR
                     TGSTSLEAEEIAQDAFVIVWRKAAQFDPVRASVGSWLFTIARNLRIDRRRAIHDTWTS
                     LDDAEAQAVADTGAGQDETLDRRRHDERVRSAVAGLTHDERVLIQLSYYEDMSHARIA
                     AQLKIPLGTVKTRLRRAAACLRERLQGCRP"
     gene            1050..2009
                     /locus_tag="VIN75_25490"
     CDS             1050..2009
                     /locus_tag="VIN75_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977593.1"
                     /translation="MSEPKKFASQADLEEKKVTFSQLSEHAWAYTAEGDPNTGIVIGD
                     DAVLVADTQATPAMAADVIRRIREVTDKPIKYVVLTHYHAVRVLGASAYQPQQVIASQ
                     DTYDLIVERGEADKASEIGRFPRLFRNVETVPAGLTWPTITFQKKMTLWLGKLEVQLL
                     QPGRGHTKGDTVVWLPQERTLLSGDLVEFDATPYAGDAYFKDWPRTLENLAALKPLAL
                     VPGRGPALQGEAQVAEGLRSTAGFIADVRASVEAGVAAGKDLNAVYKDTFARLKPKYG
                     HWVIFDHCMPFDVTRCFDEVTQYADPRVWTAERDRQMWEALEG"
     gene            2063..>3504
                     /locus_tag="VIN75_25495"
     CDS             2063..>3504
                     /locus_tag="VIN75_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8977594.1"
                     /translation="MQLNELARPGGDAVDYRKVVFDYARCADQAAAQPARRPVAIVGA
                     GPVGLALAIDLAQRNVPVVVLDDDSTLSTGSRAICFAKRTLEIFDRLGCGHRMAGKGV
                     SWNVGKVFFRDQLIYSFDLLPETGHARPAFVNLQQYYVEGYLAERAAALPLVDVRWKN
                     AVSGAEQRADHVMLTIATPDGPYRLAVDYVAACDGSRSAMRRLLGLESKGRTFKDRFL
                     IADVRMKHESPAERWFWFDPEFHRNQSVLLHRQPDGVWRIDFQLGWDADPEEEKKPER
                     ILPRVKALLAHSAMKDQDFELEWASVYTFSCLRMDSFRHGRVLFAGDSAHGVSPFGAR
                     GANSGVQDADNLAWKLAAVLGGHAPEALLDTYAREREFAADENIRHSTRATDFITPKS
                     PISRVFRDAVLELAKSHPFARTLVNSGRLSTPSTLHGSSLNTPDVDAFRGAMAPGAPA
                     ADAPVTLPDGTPSWLLAQLGAGFTVLVAGEV"
BASE COUNT          537 a         1261 c         1223 g          483 t
ORIGIN      
        1 ccgccggcca gcgcctgcgg cggttcggcc tggagcagct cgccgcccac gcgctcgaat
       61 gcgcgcacgg cctgccggca gcgcgcacag ccttcgaggt gcgcctcgac cagcagcgcc
      121 ggcccggtgg ccaggccgcc ccccgcgtgc ccgagcagca gggcgtcgtc gggatgatgg
      181 cggatcatgg ccggcatccc tggagccgct cgcgcaggca ggccgccgcg cgacggagcc
      241 tggtcttgac ggtgcccagc ggaatcttca gttgcgcggc gatgcgggcg tgcgacatgt
      301 cttcgtaata ggaaagctgg atcaggacgc gctcgtcgtg ggtcaggccg gccacggccg
      361 agcgcacgcg ctcgtcgtgc cggcgccggt cgagggtctc gtcctggccg gcgccggtgt
      421 cggcaacggc ctgggcctcg gcgtcgtcca gcgaggtcca ggtgtcgtgg atggcgcgcc
      481 gacgatcgat gcgcaggttg cgggcgatcg tgaacagcca cgatcccacg ctggcgcgca
      541 cgggatcgaa ctgcgccgcc ttgcgccaga cgatgacgaa ggcgtcctgg gcgatctcct
      601 cggcctccag gctggtgctg ccggtacgcc acagccagcc cttgagccgg ggcgcgaaat
      661 gctcgaacag ggacccgaac gccgcgcgat cctggtggcg ggccaccgcg atcatccagt
      721 cgtccagccg cgtgtcgcgg cccggcgccc ggtcatcggc agtcgacacg accagccgca
      781 ggtgcggcat gccctcctcc ggctcgctcg gccggagggg cgtcggttgc tgggaatgct
      841 cggcgtccat gaggtgatta cgggcgaggc ccggaatcgg attttgcgga ctgccgggcg
      901 cgtcgtcgat cccggcgcga tccggcggca cgccgcggcc gccgccagcg ccggcgcccg
      961 tccgcgatcg cgaggacgga tgtccacaaa aggatcaata ctggcggacg catcgagcaa
     1021 agactcctct cttcactgcc ggagaccgca tgtccgaacc caagaaattc gcctcccagg
     1081 ccgacctcga ggaaaagaag gtcaccttca gccagctgtc cgagcacgcc tgggcgtaca
     1141 ccgccgaggg cgatccgaac accgggatcg tcatcggcga cgacgccgta ctggtcgccg
     1201 acacccaggc cacgcccgcg atggccgccg acgtcatccg gcgcatccgc gaggtgacgg
     1261 acaagccgat caagtacgtc gtgctcacgc actaccacgc ggtgcgcgtg ctcggcgcca
     1321 gcgcatacca gccgcagcag gtcatcgcca gccaggacac ctacgacctg atcgtcgagc
     1381 gcggcgaggc cgacaaggcc agcgagatcg gccgcttccc gcgcctgttc cgcaacgtcg
     1441 agacggtgcc cgccggcctc acttggccca cgatcacgtt ccagaagaag atgacgctgt
     1501 ggctcggcaa gctcgaggtg cagctgctgc agccgggccg cggccacacc aagggcgaca
     1561 ccgtcgtctg gctgccgcag gagcgcacgc tgctcagcgg cgacctggtc gagttcgacg
     1621 ccacgcccta tgccggcgac gcctacttca aggactggcc gcggacgctg gagaacctcg
     1681 ccgcgctgaa gccgctggcg ctcgtcccgg gtcgcggccc ggcgctgcag ggcgaggcgc
     1741 aggtggccga gggactgcgc tccacggccg gcttcatcgc cgacgtgcgc gccagcgtcg
     1801 aggccggcgt cgccgcgggc aaggatctca acgcggtcta caaggacacg ttcgcccggc
     1861 tcaagcccaa gtacggccac tgggtgatct tcgaccactg catgccgttc gacgtcacgc
     1921 gctgcttcga cgaggtcacg cagtacgccg acccgcgtgt ctggacggcc gagcgcgacc
     1981 gccagatgtg ggaggcgctg gaaggctgaa gggccgccgc cctgcacgac ggaggcgtcc
     2041 gccgaggcgc gaggagacaa gcatgcaact gaacgaactc gcgcgtcctg gcggcgatgc
     2101 ggtggactac cggaaggtgg tcttcgacta cgcgcgctgc gccgaccagg cggccgcgca
     2161 gcccgcgcgg cggccggtgg ccatcgtcgg cgccggcccc gtgggcctgg cgctggccat
     2221 cgacctcgcg cagcgcaacg tgcccgtggt ggtgctggac gacgacagca cgctctcgac
     2281 gggctcgcgc gccatctgct ttgccaagcg cacgctggag atcttcgacc gcctgggctg
     2341 cggccaccgc atggccggca agggcgtgtc gtggaacgtg ggcaaggtgt tcttccgcga
     2401 ccagctgatc tacagcttcg acctgttgcc cgagacgggc cacgcgcgac ccgcgttcgt
     2461 caacctgcag cagtactacg tcgagggcta cctcgccgaa cgcgccgcgg cgctgccgct
     2521 ggtggacgtg cgctggaaga acgccgtcag cggcgccgag cagcgcgcgg accacgtgat
     2581 gctgaccatc gccacgcccg acggccccta ccgcctcgcg gtcgactacg tggccgcctg
     2641 cgacggctcg cgatcggcga tgcgccgcct gctgggcctc gaatcgaagg ggcgcacctt
     2701 caaggatcgc ttcctcatcg ccgacgtgcg catgaagcac gaatcgccag ccgaacgctg
     2761 gttctggttc gacccggagt tccaccgcaa ccagagcgtt ctgctgcacc gccagcccga
     2821 cggcgtctgg cgcatcgact tccagctggg ctgggatgcc gaccccgagg aagagaagaa
     2881 gcccgagagg atcctgccgc gcgtcaaggc gttgctcgcg cattcggcga tgaaagacca
     2941 ggacttcgag ctcgaatggg ccagcgtcta cacgttctcg tgcctgcgca tggacagctt
     3001 ccgccacggc cgcgtgctgt tcgccggcga ctccgcgcac ggcgtgtcgc cgttcggggc
     3061 gcgcggcgcg aactcgggcg tgcaggacgc cgacaacctg gcatggaagc tcgccgcggt
     3121 gctgggcggc cacgcgccgg aggcgctgct ggacacctac gcccgcgagc gcgagttcgc
     3181 ggccgacgag aacatccgcc actcgacccg cgcgaccgac ttcatcacgc ccaagagccc
     3241 catcagccgc gtgttccgcg acgcggtgct cgagctcgcg aagtcgcatc cgttcgcgcg
     3301 cacgctggtc aacagcggcc ggctgtcgac gccgtcgacg ctgcacggct cctcgctgaa
     3361 cacgcccgac gtcgacgcgt tccgcggcgc gatggcgccc ggcgcgcctg cggccgatgc
     3421 gcccgtcacc ctgccggacg gcacgccgtc gtggctgctg gcgcagctgg gcgcgggatt
     3481 caccgtgctc gtggcgggcg aggt
//
LOCUS       DATCRO010000934         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_820751, whole genome shotgun sequence.
ACCESSION   DATCRO010000934 DATCRO010000000
VERSION     DATCRO010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820751"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1761
                     /locus_tag="VIN75_25500"
     CDS             46..1761
                     /locus_tag="VIN75_25500"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY8977595.1"
                     /translation="MPNWLLSLDRFFPIRYLAWILCAVVALLGAFTEVVAHTGWSWAV
                     LGLAGVIVGIRDVRQRRHSILRNYPVIGHLRFFFEFIRPEMRQYFIEGDNEAAPFSRQ
                     QRSLVYQRAKGDPDKRPLGTQLDVQSEGYEWINHSLQPTKLASHDFRVTIGANCAQPY
                     HASIFNISAMSFGALSGAAIRALNGGALRGNFAHDTGEGSISVHHRAQGGDLIWEVAS
                     GYFGCRNPDGTFNAELFARHATEPQVKMIEVKLSQGAKPGHGGVLPGPKVTREIAEAR
                     GVQQGVDCVSPASHSAFSTPVEMLQFIARLRELSGGKPAGFKFCIGHPWEWFAIAKAM
                     LETGLLPDFIVVDGGEGGTGAAPLEFADHVGTPVQEGLLLVHNTLVGLNLRDRIKIGC
                     AGKVVSAFDIARMMALGANWCNSARGFMFALGCIQSQSCHTDHCPTGVATQDPLRQKA
                     LVVPSKIERVHNYHRHTLEALKELVQAAGLQHPNEITANHVVRRVAGDKVKLLANHLE
                     FIAPGALLAAARGECDWPHNVYRMYWPIARADSFAPASAAASVSAPLATPPTAAAQSA
                     LDLAV"
     gene            complement(1772..4627)
                     /locus_tag="VIN75_25505"
     CDS             complement(1772..4627)
                     /locus_tag="VIN75_25505"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY8977596.1"
                     /translation="MNNARTIRARALGGCLVAAAWPLLAAAQPAGSPAHAKVAPRAAS
                     AAVAAPPTFTIERTPAWAHPLPVDPSVPVPAAPYQLLVAEEQVRVLDHGSEHYRHTIR
                     QINDTSALQQGGQIELTFDPAYQRLVFHQVDIVRGNQRINKLDPRKVRLLQREQQLER
                     QVVDGRFTASLVLDDLRAGDRVEWSATVIGDNPVFGGRFVDTEWDAYPNAPTGIWHLR
                     LVAPAARDIHVRVGEPATTTVTTRVHDGLRETDFKRTSIAQLLSDERLPPAEYLKWQV
                     ELSEFADWKEVSDWAAQLFARAMQPSPAVDAQVAELKAKAHDRDALLRATLDFVQRDI
                     RYFGTEGGTDSHQPAAADTVLRQRFGDCKDKVALLAELLTRMGFEATPVIVSAQFRAA
                     SGTRLPGPLAFDHAIVRVTVDGKPLYLDATRAYQTGRVASREALDLGVGLLAQAGTPA
                     LAELPGGRDAVRIETLDTFAFPKLAREGTLTSVTTLHGEWAEWFRASLASQPRADIEK
                     AVQADLVHAYPSLVVSAPMDVAADGEDNTVKVTSHFRTGEFWTLPEQRALVGRYALGT
                     LVSPLRLPDQAARTQAYRLQTGRFLQHVAFDFGEPFPGVQNTPRFDETNDVFELHTHS
                     EIQSPRQDMYGELHLLTDTIPASAWSAHRDRVLKVGAHLGGSVIVSAFSADQFAALRS
                     EGEVLLAKLRKGDLHLATRAQADATMKLLMLDKELDSDRLTPPLRAQVLLAKGIALDN
                     VGKQDLGKGSIESALMLDPASADAHAALADNALARGDDKTVLREIGETLKLSPSKTEV
                     YLTRGRMHYLAGDMGPARDDFVTALQSRQEVERMYGTIWLYLAQRRAGSDAAKAVDAV
                     RAFEPSAAYPEWPYSVLQLMEGRTSLDKALAASQENGQRSAGRECELYYYAGEKALAD
                     GDLASARKYLRQSVATGVTEFVEYQSAQRELARIGNP"
BASE COUNT          647 a         1690 c         1738 g          717 t
ORIGIN      
        1 cgacccgccg ttgcccggca cgagaacctg cacgagacct tgcccatgcc caactggctc
       61 ctgtcgctcg atcgattctt cccgatccgc tatctcgcgt ggatcctgtg cgccgtcgtc
      121 gcgctgctgg gagcgttcac cgaggtcgtg gcgcacaccg gctggagctg ggcggtgctc
      181 gggctggcgg gcgtgatcgt cggcatccgc gacgtgcgcc agcgccgcca ctcgatcctg
      241 cgcaactacc cggtgatcgg ccacctgcgc ttcttcttcg agttcatccg accggagatg
      301 cgccagtact tcatcgaggg cgacaacgag gccgcgccgt tctcgcgcca gcagcgctcg
      361 ctcgtctacc agcgcgccaa gggcgacccc gacaagcgcc cgctgggcac gcagctggac
      421 gtgcagtccg agggctacga gtggatcaac cattcgctgc aaccgaccaa gctggcctcg
      481 cacgactttc gcgtcaccat cggcgcgaac tgcgcgcagc cgtaccacgc gagcatcttc
      541 aacatcagcg cgatgagctt cggcgcgctg agcggcgcag ccatccgcgc gctcaacggc
      601 ggcgcgctgc gcggcaactt cgcgcacgac accggcgagg gctcgatcag cgtgcaccat
      661 cgcgcgcagg gtggcgacct gatctgggaa gtcgcctcgg gctacttcgg ctgccgcaac
      721 ccggacggca cgttcaacgc ggagctgttc gccaggcacg ccaccgagcc gcaggtcaag
      781 atgatcgagg tcaagctgag ccagggcgcc aagccgggcc acggcggcgt gctgccgggc
      841 ccgaaggtga cccgggagat cgccgaggcg cgcggcgtgc agcagggcgt ggactgcgtc
      901 tcgccggcca gccacagcgc gttctcgacg cccgtcgaga tgctgcagtt catcgccagg
      961 ctgcgcgagc tctcgggcgg caagccggcc ggcttcaagt tctgcatcgg ccatccgtgg
     1021 gaatggttcg ccatcgccaa ggccatgctg gagacaggcc tgctgcccga cttcatcgtc
     1081 gtggacggcg gcgagggcgg caccggcgcc gcgccgctgg aattcgccga ccacgtgggc
     1141 acgcccgtgc aggaaggcct gctgctggtg cacaacacgc tcgtcggcct gaacctgcgc
     1201 gacaggatca agatcggctg cgccggcaag gtggtcagcg cgttcgacat cgcgcgcatg
     1261 atggcgctcg gcgcgaactg gtgcaattcg gcgcgcggct tcatgttcgc gctcggctgc
     1321 atccagtccc agagctgcca caccgaccac tgccccacgg gcgtggccac gcaggatccg
     1381 ctgcgccaga aggcgctggt ggtgccctcc aagatcgagc gggtgcacaa ctaccaccgc
     1441 cacacgctgg aggcgctcaa ggagctggtg caggccgcgg ggctgcagca tcccaacgag
     1501 atcaccgcca accacgtggt gcgccgcgtc gcgggcgaca aggtcaagct gctggccaac
     1561 cacctcgagt tcatcgcgcc cggcgcgctg ctggcggccg cgcgcggcga atgcgattgg
     1621 ccgcacaacg tctacaggat gtactggccg atcgcgcgtg ccgacagctt cgcgccggcc
     1681 agcgccgccg cgtccgtgag tgcgcccctg gcgacgccgc cgacggccgc cgcgcagtcg
     1741 gcgctcgacc tcgccgtctg agccgcggct ttcaggggtt gccgatgcgc gccagctcgc
     1801 gctgggccga ctggtactcg acgaactcgg tcacgccggt ggccacgctc tggcgcaggt
     1861 acttgcgcgc gctggccagg tcgccgtcgg cgagcgcctt ctcgcccgcg tagtagtaca
     1921 gctcgcactc gcggcccgcg ctgcgctggc cgttctcctg cgaggccgcg agcgccttgt
     1981 ccaggctggt gcggccctcc atcagctgca gcacgctgta cggccattcc ggatacgccg
     2041 cggacggctc gaacgcgcgg accgcgtcca cggccttggc cgcgtcgctg ccggcgcggc
     2101 gctgcgccag gtacagccag atcgtgccgt acatgcgctc gacctcctgg cgcgactgca
     2161 gcgcggtgac gaagtcgtcg cgcgccggac ccatgtcgcc ggccaggtag tgcatgcggc
     2221 cgcgtgtcag gtagacctcg gtcttggacg gcgacagctt gagcgtctcg ccgatctcgc
     2281 gcagcaccgt cttgtcgtcg ccgcgcgcca gtgcgttgtc ggccagcgcc gcgtgcgcgt
     2341 cggcgctggc cgggtccagc atcagcgccg attcgatcga acccttgccc aggtcctgct
     2401 tgccgacgtt gtccagcgcg atgcccttgg ccagcagcac ctgcgcgcgc agcggcggcg
     2461 tgaggcggtc ggagtcgagc tccttgtcga gcatcagcag cttcatcgtg gcgtcggcct
     2521 gtgcccgcgt ggccaggtgc aggtcgccct tgcgcagctt ggccagcagg acctcgccct
     2581 cgctgcgcag cgcggcgaac tggtcggcgg agaacgccga gacgatgacg ctgccgccca
     2641 ggtgcgctcc gaccttgagc acgcggtcgc ggtgcgccga ccacgcgctg gcggggatgg
     2701 tgtcggtgag caggtgcagc tcgccataca tgtcctgccg cggggactgg atctccgaat
     2761 gcgtgtgcag ctcgaagacg tcgttcgtct cgtcgaagcg cggcgtgttc tgcacgcccg
     2821 ggaacggctc gccgaagtcg aacgcgacgt gctgcaggaa gcgcccggtc tgcagccggt
     2881 aggcctgcgt gcgcgccgcc tggtcgggca ggcgcagcgg cgagacgagc gtgcccagcg
     2941 cgtagcggcc gaccagcgcg cgctgctcgg gcagcgtcca gaactcgccg gtgcgaaagt
     3001 gcgaggtgac cttgacggtg ttgtcctcgc cgtcggcggc cacgtccatc ggcgcgctga
     3061 ccaccagcga cgggtaggcg tgcacgaggt cggcctggac cgccttctcg atgtcggcgc
     3121 gcggctggct ggccaggctc gcgcggaacc actcggccca ttcgccgtgc agtgtggtca
     3181 ccgacgtcag cgtgccctcg cgcgccagct tcgggaacgc gaaggtgtcg agcgtctcga
     3241 tgcgcaccgc gtcgcggccg ccgggcagct cggcgagcgc cggcgtgccg gcctgcgcga
     3301 gcagcccgac gccgaggtcc agcgcctcgc gcgacgcgac gcggccggtc tggtaggcgc
     3361 gcgtcgcgtc caggtacagg ggcttgccgt cgaccgtcac gcgcacgatc gcgtggtcga
     3421 acgcgagcgg gcccggcagg cgggtgccgc tggcggcccg gaactgcgcg gacacgatca
     3481 ctggcgtcgc ctcgaagccc atgcgcgtga gcagctcggc cagcagcgcg accttgtcct
     3541 tgcaatcgcc gaaccgctgg cgcagcaccg tgtcggccgc ggccggctgg tgcgagtcgg
     3601 tgccgccctc ggtgccgaag tagcggatgt cgcgctggac gaagtcgagc gtcgcgcgca
     3661 gcagcgcgtc gcggtcgtgc gccttggcct tgagctcggc cacctgggcg tcgacggcgg
     3721 ggctgggctg catcgcgcgg gcgaacagct gcgcggccca gtccgacacc tccttccagt
     3781 cggcgaactc gctgagctcg acctgccact tcaggtattc ggcgggcggc aggcgctcgt
     3841 cggacagcag ctgcgcgatg ctcgtgcgct tgaagtcggt ctcgcgcagg ccgtcgtgca
     3901 cgcgggtcgt gaccgtcgtc gtggccggct cgccgacgcg cacgtggatg tcgcgcgcgg
     3961 ccggcgccac gaggcgcagg tgccagatgc cggtgggcgc gttcgggtag gcgtcccact
     4021 cggtgtcgac gaagcggccg ccgaacacgg ggttgtcgcc gatgacggtg gccgaccact
     4081 cgacgcggtc gccggcgcgc aggtcgtcga gcaccagcga cgcggtgaag cggccgtcga
     4141 ccacctgtcg ctccagctgc tgctcgcgct gcagcaggcg caccttcctc gggtcgagct
     4201 tgttgatgcg ctggttgccg cggacgatgt cgacctggtg gaacacgagg cgctggtagg
     4261 cggggtcgaa ggtcagctcg atctgcccgc cctgctgcag cgcggacgtg tcgttgatct
     4321 ggcggatggt gtggcgatag tgttcggagc cgtgatcgag cacgcgcacc tgttcctcgg
     4381 ccacgagcag ctggtagggc gccgcgggca ccggcacgga cgggtcgacc gggagcgggt
     4441 gcgcccatgc cggcgtgcgc tcgatcgtga acgtgggcgg cgccgccacc gcggcggacg
     4501 cggcgcgcgg cgcgaccttc gcgtgggccg ggctgccggc aggctgcgcc gcggcgagca
     4561 gcggccaggc cgcggcgacg aggcagcctc cgagcgcgcg cgcgcggatc gttcgtgcgt
     4621 tgttcatgcc atccctccca ggcgtcttgt cgggcgattc tcgcagtgcg gccgcgggag
     4681 cgcgcgacca ggccgccgcg cgggcatgaa aaaaggcggc cttcggccgc cttcgtcgcg
     4741 tgcgccgcac ggctcaggcc gtgggcggcg cggtgtcgat gaagctctgg cg
//
LOCUS       DATCRO010000935         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_33342, whole genome shotgun sequence.
ACCESSION   DATCRO010000935 DATCRO010000000
VERSION     DATCRO010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33342"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1624)
                     /gene="lysS"
                     /locus_tag="VIN75_25510"
     CDS             complement(128..1624)
                     /gene="lysS"
                     /locus_tag="VIN75_25510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8977597.1"
                     /translation="MDTTTPPTDENKLIAERREKLAAIRAQGGVAFPNDFKPADRAAD
                     LVARYGKLTNEELEPQDIHVSVGGRMMLKRTMGKAAFCSLQDATGRIQLRVAIDNVGE
                     QTYAAFKHWDLGDILGAEGSLFITKTGELTIKVTKLRLLTKSLRPLPDKFHGVSDQEV
                     KYRQRYVDLITDEEARKRFRARSDALGSIRAFMVANGFMEVETPMLHPIPGGANAKPF
                     VTHHNALDQAMYLRIAPELYLKRLIVGGFERVFEINRSFRNEGISVRHNPEFTMMEFY
                     AAYWNHHDLMDFTEQVLRHAAIAATGSAALTYAGKDVHLDKPFARLTVRDSLVEHCGL
                     TLAQTEDKALLRERILAAGEKAHEHWTLAELQFGLFEAEVEAKLWQPTFIIDYPVEVS
                     PLARASDNNPAITERFELFITGREYANGFSELNDAEDQAARFQAQVANKDAGDEEAMF
                     YDADFIRALEYGMPPTGGCGIGIDRLMMLLTDSASIRDVILFPALRKE"
     gene            1955..3034
                     /locus_tag="VIN75_25515"
     CDS             1955..3034
                     /locus_tag="VIN75_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8977598.1"
                     /translation="MSPHSLRPQTHAPVTDRKVRFALIGCGRIAANHMESIAKHAERA
                     ELVAVCDSNAERLAAAVQRTGARGYRSLQELLERETPDCVVITTPSGLHSQQVIEVAR
                     AGFNAMTEKPMATRWQDGLSMVRACDEAGVRLFVVKQNRRNKTLQLLKQAMELGRFGR
                     IYTIAVNVFWTRPQEYYDSAAWRGTWEFDGGAFMNQASHYIDLLDWIGGPVESVMAYT
                     GTLARNIEVEDTGVAALKWRNGAMGTLNVTMLTYPKNLEGSITILGEKGTVRIGGVAV
                     NEIQQWQFAEPHPMDAERDDASYQTTSVYGFGHPLYYDNVISTLRGEAQAETDGREGL
                     RSLEMLIAMYMSARDGKRVNLPLEY"
     gene            3034..3624
                     /locus_tag="VIN75_25520"
     CDS             3034..3624
                     /locus_tag="VIN75_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY8977599.1"
                     /translation="MPTTIHPTAIVDDGAVLGDNCRVWHWVHISGGARIGAGCSFGQN
                     VFVGNDVAIGNNVKIQNNVSVYDAVTLEDDVFCGPSMVFTNVYNPRSAVTRKNEYRRT
                     LVRQGATLGANSTLVCGVTVGRQAFIGAGAVVQKDVPDFALMVGVPARQIGWMSRFGE
                     RLELPLVAPAGQPVETTCPNNGDRYRLDGRVLSLIS"
     gene            3667..>4358
                     /locus_tag="VIN75_25525"
     CDS             3667..>4358
                     /locus_tag="VIN75_25525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8977600.1"
                     /translation="MEFIDLKAQYAVLRDEIAVRMQKVLDHGQYIMGPEVKELEAALA
                     AHTGAKHCITVASGTEALLISLMALDLKPGDEVVTTPFTFAATAETIVLLGGVPVWVD
                     IEPDTCNIDASKIEAAITPRTRAIMPVSLYGQCADMDEINAIAARHGLPVIEDAAQSF
                     GADYHGRKSCNLSTLGCTSFFPSKPLGCYGDGGAIFTSDDALAQAAREIRVHGQSARY
                     THTRVGVGGRMDT"
BASE COUNT          691 a         1515 c         1480 g          672 t
ORIGIN      
        1 gacagcgcca gcatccgcga cgtgatcctg ttccccgcac tgcgcaagga ataggcccca
       61 cgctcgtgga cgagcgcacg acgttcgccg cccccgaggg ggcagcgagc gccagcgagc
      121 gtggggccta ttccttgcgc agcgcgggaa acaggatcac gtcgcggatg ctggcgctgt
      181 cggtcagcag catcatcagg cggtcgatgc cgatgccgca gccgcctgtc gggggcatgc
      241 cgtattcgag cgcgcggatg aagtcggcgt cgtagaacat cgcctcctcg tcgccggcgt
      301 ccttgttggc gacctgcgcc tggaagcgcg cggcctggtc ctcggcgtcg ttcagctccg
      361 agaagccgtt ggcgtactcg cggccggtga tgaacagctc gaagcgctcc gtgatggccg
      421 ggttgttgtc ggacgcgcgc gccagcgggc tcacctcgac cgggtagtcg atgatgaacg
      481 tcggctgcca caacttcgcc tcgacttccg cctcgaacag gccgaactgc agctcggcca
      541 gcgtccagtg ctcgtgcgcc ttctcgccgg cggccaggat gcgctcgcgc agcagcgcct
      601 tgtcctcggt ctgcgccagg gtgaggccgc agtgctcgac cagcgagtcg cgcacggtca
      661 ggcgcgcgaa cggcttgtcc aggtgcacgt ccttgccggc ataagtgagc gcggccgagc
      721 cggtggcggc gatcgccgca tggcgcagca cctgctcggt gaagtccatc aggtcgtggt
      781 ggttccagta cgccgcgtag aactccatca tcgtgaattc ggggttgtgc cgaaccgaga
      841 tgccttcgtt gcggaagctg cggttgatct cgaacacgcg ctcgaagccg cccacgatca
      901 ggcgcttgag gtagagctcg ggcgcgatgc gcaggtacat cgcctggtcg agcgcgttgt
      961 ggtgcgtgac gaacggcttg gcgttcgcgc cgcccgggat cgggtgcagc atgggcgtct
     1021 cgacttccat gaagccgttg gccaccatga acgcgcggat cgaaccgagc gcgtcgctgc
     1081 gcgcgcggaa gcgcttgcgc gcctcctcgt cggtgatcag gtcgacatac cgctggcggt
     1141 acttcacttc ctggtcggac acgccgtgga acttgtcggg cagcggacgc agcgacttgg
     1201 tcagcaggcg cagcttggtg accttgatcg tgagctcgcc ggtcttggtg atgaacagcg
     1261 agccttcggc gcccaggatg tcgccgaggt cccagtgctt gaacgcagcg taggtctgct
     1321 cgccgacgtt gtcgatcgcc acgcgcagct ggatacggcc ggtggcatcc tgcagcgagc
     1381 agaacgcggc cttgcccatc gtgcgcttga gcatcatgcg gccgcccacg ctgacgtgga
     1441 tgtcctgcgg ctccagctcc tcgttggtca gcttgccgta cctggccacc aggtcggccg
     1501 cgcggtcggc cggcttgaag tcgttcggga acgccacgcc gccctgggcg cggatcgcgg
     1561 ccagtttctc gcgacgctcc gcaatcagct tgttctcgtc ggtcgggggc gtcgtggtgt
     1621 ccatgcttgg gaatcgatgc gggaagggtg gcgccggcgc ggcggccggc gggaaggggg
     1681 cagcgtgcgc tgccaggggg aacccgcgat tttacgtgga tcggacggcg cgcgcggggc
     1741 acgtccgccc gctgtcttcg cgcggcgcga agcgattccc ccccgcacgc ccacccgcca
     1801 acctgtcagg cccctatcgc cttgccgggc cgggtgcctt aaaatggcgg attgccccat
     1861 tcttcccgtg cccgccgcgg gctgaaggcg cgccgcgcgg tcccgggccg cctggctgtt
     1921 gcgcgccccc tccctgaagt gcccgtcgtc cgtcatgtct ccccatagcc tccgccccca
     1981 gacccacgct cccgtcaccg accgcaaggt ccgcttcgcg ctgatcggct gcggacggat
     2041 cgcggccaac cacatggaat cgatcgccaa gcacgccgag cgtgccgagc tcgtcgccgt
     2101 gtgcgattcc aacgcggagc gcctggccgc cgccgtgcag cgcaccggcg cgcgcggcta
     2161 ccgcagcctg caggagctgc tggaacgcga gacgcccgac tgcgtcgtga tcaccacgcc
     2221 cagcggcctg cactcgcagc aggtgatcga ggtggcgcgc gccggcttca acgccatgac
     2281 cgagaagccc atggccacgc gctggcagga cggcctgtcg atggtgcgcg cctgcgacga
     2341 ggccggcgtg cgcctgttcg tcgtcaagca gaaccgccgc aacaagacgc tgcagctgct
     2401 caagcaggcc atggaactgg gccgcttcgg ccgcatctac acgatcgcgg tcaacgtgtt
     2461 ctggacgcgt ccgcaggagt actacgactc ggccgcctgg cgcggcacct gggaattcga
     2521 cggcggcgcc ttcatgaacc aggccagcca ctacatcgac ctgctcgact ggatcggcgg
     2581 cccggtcgag agcgtgatgg cctacaccgg cacgctggcg cgcaacatcg aggtggagga
     2641 caccggcgtc gccgcgctca agtggcgcaa cggcgccatg ggcacgctca acgtgaccat
     2701 gctcacgtac ccgaagaacc tcgagggctc gatcaccatc ctgggcgaga agggcacggt
     2761 gcgcatcggc ggcgtggcgg tcaacgagat ccagcagtgg cagttcgccg agccgcatcc
     2821 gatggacgcc gagcgcgacg acgcgagcta ccagacgacc tccgtctacg gcttcggcca
     2881 cccgctgtac tacgacaacg tcatcagcac gctgcgcggc gaggcccagg ccgagaccga
     2941 cggccgcgaa ggccttcgct cgctggagat gctgatcgcg atgtacatgt ccgcacgcga
     3001 cggcaagcgc gtcaacctgc cgctcgaata ctgatgccca cgaccatcca cccgaccgcc
     3061 atcgtcgacg acggcgcggt cctcggcgac aactgccgcg tctggcactg ggtgcacatc
     3121 agcggcggcg cgcgcatcgg cgcgggctgc tccttcggcc agaacgtgtt cgtcggcaac
     3181 gacgtggcca tcggcaacaa cgtcaagatc cagaacaacg tgtcggtgta cgacgcggtg
     3241 acgctggagg acgacgtctt ctgcgggccc agcatggtgt tcaccaacgt gtacaacccg
     3301 cgctcggcgg tcacgcgcaa gaacgagtac cgccgcacgc tggtgcgcca gggcgcgacg
     3361 ctcggcgcga actccacgct ggtgtgcggc gtcaccgtcg gccgccaggc cttcatcggc
     3421 gccggcgcgg tggtgcagaa ggacgtgccg gacttcgcgc tgatggtggg cgtgcccgcg
     3481 cgccagatcg gctggatgag ccgcttcggc gaacggctcg agctgccgct cgtggcgccc
     3541 gccggccagc cggtcgagac cacctgcccc aacaacggcg accgctatcg cctcgacggc
     3601 cgcgtgctgt cgctgatctc ctgaaccgac acgaccatga acgctccgct tccgccgcaa
     3661 ggcaagatcg aattcatcga cctcaaggcc cagtacgccg tgctgcgcga cgagatcgcc
     3721 gttcgcatgc agaaggtgct ggaccacggc cagtacatca tgggccccga ggtcaaggag
     3781 ctcgaggccg cgctggccgc gcataccggc gcgaagcact gcatcacggt ggccagcggc
     3841 accgaggcgc tgctgatctc gctgatggcg ctggacctga agccgggcga cgaggtcgtc
     3901 accacgccgt tcacgttcgc cgccaccgcc gagaccatcg tgctgctggg cggcgtgccg
     3961 gtgtgggtcg acatcgagcc cgacacctgc aacatcgacg cctcgaagat cgaggccgcg
     4021 atcacgccgc gcacccgcgc catcatgccg gtgagcctgt acggccagtg cgcggacatg
     4081 gacgagatca acgcgatcgc cgcgcgccac ggcctgcccg tgatcgagga cgcggcgcag
     4141 agcttcggtg ccgattacca cggccgcaag agctgcaacc tgagcaccct cggctgcacc
     4201 agcttctttc ccagcaagcc gctcggctgc tacggcgacg gcggcgccat cttcaccagc
     4261 gacgacgcgc tggcgcaggc ggcgcgcgag atccgcgtgc acggccagag cgcgcgctac
     4321 acgcataccc gggtgggtgt gggcgggcgc atggacac
//
LOCUS       DATCRO010000936         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_708111, whole genome shotgun sequence.
ACCESSION   DATCRO010000936 DATCRO010000000
VERSION     DATCRO010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708111"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VIN75_25530"
     CDS             complement(<1..288)
                     /locus_tag="VIN75_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY8977601.1"
                     /translation="MNPWLGLLAIVGLAAITVITRGFFIIPEREIPIPGWLREALRYA
                     PLAALVAVLLPEVAMTGDHLLDTWRDARIYATVTGVVVFAATRNLLGTILAG"
     gene            complement(285..1025)
                     /locus_tag="VIN75_25535"
     CDS             complement(285..1025)
                     /locus_tag="VIN75_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY8977602.1"
                     /translation="MTALPATWRHPEFARGARDMTGISLGVGAWGLVTGIAMVKAGLG
                     FWLSLAMSLFVFAGSAQLASLPLIAQGAPLWVIWATAFCVNLRFIIFSAGWRPYLAPL
                     SRWRRMWVMYFMADLNYVAFMKRFPVPGPMDEQLPFLWGGVAVNWAAWQGMSILGILL
                     ADVIPAQWGLGFAGSLALLGIVGTLLVDRGTWIAAAVAACAAFAAFALPFHLNIVVAI
                     AAAVGIGVLVDHFSPPAPAHATAGSDAS"
     gene            complement(1123..1569)
                     /locus_tag="VIN75_25540"
     CDS             complement(1123..1569)
                     /locus_tag="VIN75_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977603.1"
                     /translation="MLTRLLPLLAIPFVLAGCDQLGLEPASVTAARVEADGKAVGAAC
                     RHGGRAIEDCFTLNKRVDKAAIYAGWREMDDYMRENKLEAVAPTLGAPVRKADDGAAD
                     ASTAKADGDKAADKPVDKPVKAAAKGDKAEKVAKADKADGEKPAEH"
     gene            complement(1660..2613)
                     /gene="fmt"
                     /locus_tag="VIN75_25545"
     CDS             complement(1660..2613)
                     /gene="fmt"
                     /locus_tag="VIN75_25545"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8977604.1"
                     /translation="MRVAFAGTPEFAAVALKAIHEAGHEVPLVLTQPDRPAGRGMRLT
                     PSPVKQLALELDAPVAQPRSLRLDGKFPDDAQAGRAALDAARPDVLVVAAYGLILPQW
                     VLDLPRLGCLNIHGSLLPRWRGAAPIHRAIEAGDAETGITIMQMDAGLDTGDMLLVER
                     EAILADDTTGRLHDRLAALGARMIVRALADAGAGALKPVKQPGQGVTYAHKIDKAEAA
                     IDWTLPADAIERRVRAFDPFPGATFQAGGDTVKLWRAQVERLDDTAAPGTVLAVDGQQ
                     LVVACGDGALALLDVQRPSGKRGPVAQWLQAHPVQRGSVLG"
     gene            complement(2628..3140)
                     /gene="def"
                     /locus_tag="VIN75_25550"
     CDS             complement(2628..3140)
                     /gene="def"
                     /locus_tag="VIN75_25550"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8977605.1"
                     /translation="MSQLQILRYPDPRLYTVAKPVAEVTDEIRALADEMIQVMYASDG
                     VGLAATQVDRHIRLIVMDTSETHDQPRVLINPELVESSDDWVLGEEGCLSVPQVYEKV
                     ERHARVRVKALDRDGKPYEFDAEGLAAVCVQHEMDHLMGKVFVDYLSPLKRDRIKTKM
                     IKKTREEAKR"
BASE COUNT          500 a         1155 c         1225 g          499 t
ORIGIN      
        1 acccgcgagt atcgttccga gcaggttgcg cgtggcggcg aacacgacga cgccggtgac
       61 ggtggcgtag atgcgggcgt cgcgccaggt gtcgagcagg tggtcgccgg tcatcgcgac
      121 ttccggcagc agcacggcca ccagcgcggc cagtggcgcg tagcgcagcg cctcgcgcag
      181 ccagccgggg atcgggatct cgcgctccgg aatgatgaag aagccgcgcg tgatcacggt
      241 gatcgcggcc aggccgacga tggccagcag gccgagccag ggattcatga cgcatcgctc
      301 cctgcggtgg cgtgcgccgg cgccggcggc gagaagtggt cgaccagcac gccgatgccg
      361 acggccgccg cgatggcgac gacgatgttc aggtggaacg gcagcgcgaa cgcggcaaac
      421 gcggcgcagg ccgcgacggc ggcggcgatc caggtgccgc ggtcgaccag cagcgtgccg
      481 acgatgccca gcaaggccag cgacccggcg aagcccaggc cccattgcgc cgggatgacg
      541 tcggccagca ggatgcccag gatcgacatg ccctgccacg ccgcccagtt cacggccacg
      601 ccgccccaca ggaagggcaa ctgctcgtcc atcggccccg gcacgggaaa gcgcttcatg
      661 aacgcgacgt agttgaggtc ggccatgaag tacatcaccc acatccggcg ccagcgggac
      721 aggggcgcca ggtacggccg ccagccggcg ctgaagatga tgaagcgcag gttgacgcag
      781 aacgccgtgg cccagatcac ccacagcggc gcgccctggg cgatcagcgg cagcgaggcg
      841 agctgggcgc tgccggcgaa cacgaacagc gacatcgcga gcgacagcca gaagccgagc
      901 ccggccttga ccatcgcgat gccggtgacg agcccccacg cacccacgcc cagcgagatg
      961 ccggtcatgt cgcgcgcgcc gcgggcgaat tcagggtggc gccacgtggc gggcaatgcg
     1021 gtcatcgcgc cattgtctta tggcggcgct cgcctcgcgc agtagcccgc tgcggctggt
     1081 ggtcaggatt gcttcgcaag gcgcgaagcg gcgcttcccg cgtcagtgtt cggcgggctt
     1141 ctcgccgtcg gccttgtcgg ccttggccac cttctcggcc ttgtcgccct tggcggcggc
     1201 cttgacgggc ttgtcgaccg gcttgtcggc ggccttgtcg ccgtcggcct tggcggtcga
     1261 cgcgtcggcc gcgccgtcgt cggccttcct gaccggcgcg cccagcgtcg gggcgacggc
     1321 ctcgagcttg ttctcgcgca tgtagtcgtc catctcgcgc cagccggcgt agatggcggc
     1381 cttgtccacg cgcttgttga gcgtgaagca gtcctcgatc gcgcggccgc cgtggcggca
     1441 ggcggcgccc acggccttgc cgtcggcctc gacgcgcgcg gccgtcaccg acgccggctc
     1501 caggcccagc tggtcgcagc cggcgagcac gaacggaatg gcgaggaggg gcaggaggcg
     1561 tgtgagcatg gcagggacgg gtcgatacgt cgttatcggc cgcccgccgg cgcacttgag
     1621 ggctcgaagc gacgaactgg cgccgctttg gccagcacac taacccagga cgctgccgcg
     1681 ctgcaccgga tgcgcctgca gccactgcgc gaccggaccg cgcttgccgc tcgggcgctg
     1741 cacgtccagc agcgccagcg cgccgtcgcc gcaggccacg accagttgct ggccgtcgac
     1801 cgcgagcacc gtgccgggcg cggccgtgtc gtccaggcgt tcgacctgcg cccgccacag
     1861 cttgacggtg tcgccgccgg cctggaaggt ggcgccgggg aacgggtcga acgcgcgcac
     1921 gcggcgctcg atcgcgtcgg ccggcaacgt ccagtcgatc gcggcctcgg ccttgtcgat
     1981 cttgtgggcg taggtgacgc cctgcccggg ctgcttcacc ggcttcagcg cgccggcgcc
     2041 ggcgtcggcc agcgcccgca cgatcatgcg ggcgccgagc gccgcgaggc ggtcatgcag
     2101 acggccggtg gtgtcgtcgg ccaggatggc ctcgcgctcg accagcagca tgtcgccggt
     2161 atcgaggccc gcgtccatct gcatgatcgt gatgcccgtc tcggcgtcgc cggcctcgat
     2221 cgcgcggtgg atcggcgcgg cgccgcgcca gcgcggcagc agcgagccgt ggatgttcag
     2281 gcaacccagg cgcggcaggt ccagcaccca ctgcggcagg atcaggccgt aggcggcgac
     2341 caccagcacg tcgggccgcg cggcgtcgag cgcggcgcga ccggcctgcg cgtcgtcggg
     2401 gaacttgccg tccagccgca ggctgcgcgg ctgggcgacg ggcgcgtcga gctcgagcgc
     2461 cagctgcttg accggcgacg gcgtgagcct catgccacgg ccggccgggc gatcgggctg
     2521 ggtcagcacg agcggcacct cgtgcccggc ctcgtggatg gccttgagcg cgaccgccgc
     2581 aaactcgggc gtgccggcaa aggcgacgcg cagcgcgggc gtcgccatca gcgcttggcc
     2641 tcctcgcgcg tcttcttgat catcttggtc ttgatgcggt cgcgcttgag cgggctgagg
     2701 tagtcgacga acaccttgcc catcaggtgg tccatctcgt gctgcacgca gacggcggcc
     2761 aggccttcgg cgtcgaactc gtagggcttg ccgtcgcggt ccaacgcctt gacgcgcacg
     2821 cgggcgtggc gctccacctt ctcgtagacc tgcggcaccg acaggcagcc ctcctcgccc
     2881 agcacccagt cgtcggagct ctcgaccagt tcggggttga tcagaacgcg cggctggtcg
     2941 tgcgtctcgg aggtgtccat gacgatcagg cggatgtggc ggtcgacctg cgtcgcggcc
     3001 aggcccacgc cgtcggacgc gtacatgacc tggatcatct cgtcggcgag cgcgcggatc
     3061 tcgtcggtga cttcagcgac cggcttggcc accgtgtaca gccgcggatc ggggtaacga
     3121 agaatctgga gttgggacat ggaaagatcg ttcctggaaa tctgccacga aaatcacgag
     3181 taatgcggcg aggacccttg cgagcgcagt gcaaaatgtg ttgagacgca acgtccggac
     3241 tcagggtgaa cgcctatctc cgtcgtaacg gagtgtccga ttcgttgcga cgtcaggggt
     3301 ttgcgggcag aatctgcggc aaatttggta gtttcttggg cggctggata ctccggccga
     3361 ttcgggattg tcgcagttc
//
LOCUS       DATCRO010000937         7180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_168303, whole genome shotgun sequence.
ACCESSION   DATCRO010000937 DATCRO010000000
VERSION     DATCRO010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168303"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..980)
                     /locus_tag="VIN75_25555"
     CDS             complement(66..980)
                     /locus_tag="VIN75_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977606.1"
                     /translation="MERSLQTAEALRRDVSCLFGHGVVRWIFCLVLSAPLSALANEVV
                     RFPVPEFEGDHRYDYATQLLQLALSKSGTAYSIEPAAFPMNQERQVLELEAGRSIDVG
                     PIPSSAEREARLLPIMIPINKGVLGWRLGLIRKGEQSSFANVRTLKDLKHVRLAQGQE
                     WPDTTILRANGIDVITAPRYEGLFKMLIGKRFDYFPRSVMEIWDEQESNASTLEVEPR
                     LALHYFYDAYFMVNRRNTRLAHDIESGLERAITDGSFDRLFQQYYGERLRKARLETRT
                     VIELRNPLTTPGTPTHRPELWYDGRRGH"
     gene            complement(1146..1349)
                     /locus_tag="VIN75_25560"
     CDS             complement(1146..1349)
                     /locus_tag="VIN75_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977607.1"
                     /translation="MSAASNSEAGAAVGSSPRVVSQIKFFIRPGIEREIRFHHFIEQF
                     ERYVPNEIGHALRSALWPGESFY"
     gene            1458..1853
                     /locus_tag="VIN75_25565"
     CDS             1458..1853
                     /locus_tag="VIN75_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977608.1"
                     /translation="MEAVQAGLDEAIDRHGKFVSGFGHRFHPIDPRAPRLLQLVDEAS
                     AQQVVNGKFAAIARGVEAELAGRKNGRRIPMNIDRCTELGASELFTGSLAPLAGAFPS
                     KGAGRALDRIASGTSGGASPDSASGDAMD"
     gene            complement(1939..3006)
                     /locus_tag="VIN75_25570"
     CDS             complement(1939..3006)
                     /locus_tag="VIN75_25570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEY8977609.1"
                     /translation="MARGASDVVDHLRGVPGAVHLRRRPLSADWIDAAPASREPRGVS
                     ISGSGPALTRQTCLTKVRYMDADAIARVRRFNRAVTVEVGALDTSFLGRGRPLGAARV
                     LNSIGRGVSDVARLREALGLDSGLASRLLRGLEDEGLVETVAHPDDARRRVVRLTRAG
                     QREFRAYEKLSDARAGAVIERHRHPEKLLAAMDVIAAALQRDRTVIEPADPRSEPARA
                     CLHAYYDELARRFASGFDVARSRDPDAADMVAPRGSFLLAWSDGVPVGCVGLKGDGGD
                     TAEVKRLWVATSTRGQGLARRLMAAVEDAARGLGVRVLRLDTNSALPEALALYRTTGW
                     IEIPRFNDDPYPDVFFEKRLA"
     gene            complement(2928..4301)
                     /locus_tag="VIN75_25575"
     CDS             complement(2928..4301)
                     /locus_tag="VIN75_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY8977610.1"
                     /translation="MHSTTQTAAVAPESAAAHTPPKPAPAWLERWFDLTRHGTTVNTE
                     LLAGLTTFLTMAYIIFVNPAILGDAGMPKGAVFVATCLIAALGSLIMGLYANYPIALA
                     PGMGLNAYFAYVVVLGMGMKWQVALGAVFISGCLFLLATVFRLRELIIQGIPQSLRIG
                     ITVGIGMFLAIIALKSAGIVAPSKATYVTLGDLHAPATILAVIGFLAVVVMDRLRVRG
                     AILIGILAVTGLSFFFAGNKFDGVVSMPPSIAPTFLQLDIKGALGVGVFNVILVFFLV
                     EVFDATGTLMAVARRAGLVTDGRMHRMNKALLADSSAIFAGSLLGTSSTTAYIESAAG
                     VQAGGRTGLTAVAVALLFLACLFFSPLAGVVPAYATAPALLYVASLMLRDLVDLDWNE
                     TTESVPAVVTALAMPLTYSIANGLAFGFITYAALKLFTGKWREAHPMSWIICAVFLVR
                     FIYVGGH"
     gene            complement(4388..5689)
                     /gene="guaD"
                     /locus_tag="VIN75_25580"
     CDS             complement(4388..5689)
                     /gene="guaD"
                     /locus_tag="VIN75_25580"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY8977611.1"
                     /translation="MTTASRIALRGDLLDFTGDPGFAAPASAAGVRFRPDHWLLVEDG
                     RIVGAQPEDPAPGWERVDRRGKLILPGFVDTHVHSPQLDVIASYGTELLEWLNTYTFP
                     AESRHGDPVAAVAGAERFLDALLAHGTTSAVVFPTVHKASADALFAGAQRRGMRIIAG
                     KVLMDRHAPDTLRDDVRGAERDCVDLIQRWHGRDRLAYALTVRFAPTSTSAQMEMAAA
                     LRRAHPGTYMQTHVAENRDEVRWVAELFPQARSYLDVYHRHGLLNERSVLAHGVWLDD
                     TDRVLLRETGAQIAHSPSSNLFLGSGLFDWTAMNAAGARVSLASDVGGGTSLSMIRTL
                     ADAYKVAAMRGVKLTAWKALYGATRGAARALHLDHEIGSLEPGRHADIAVWDWASTPL
                     MQHRVDLAQDLHEKLFAWMTLGDERLLESAYVAGVPRYRRE"
     gene            complement(5711..6652)
                     /locus_tag="VIN75_25585"
     CDS             complement(5711..6652)
                     /locus_tag="VIN75_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977612.1"
                     /translation="MAEGNSFDKIELSLVRLFHTVITERSISRAALRLQTTQPAVSAK
                     LKRLRELTGDPLLVRAGHGMVPTPAALALMEPAGDLLRSAQLMFGSRSRGLDFDPATA
                     SMHFRIAASDWLDPMLLPEVVGRIKTQAPGVTLEILPLTDEYDYRLHLSRAEVDLVVG
                     NWLQPPGELHIGQLFSDEIVCLVAADHPAVRNPRWWTSERYLESEHVAPRPLHAGAPG
                     VIDEHLAGQGLARNITVRTPHFALAPLMVARSHLVLTTGRRFCSRYVDTLPVRIVRCP
                     MRLTSLSYYQLWHELSHHSGASRWLRTQVREATQSLV"
     gene            6753..>7180
                     /locus_tag="VIN75_25590"
     CDS             6753..>7180
                     /locus_tag="VIN75_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8977613.1"
                     /translation="MQTRPIRFFHHHPGEIVEVTDAAPTRTVLNWLREDARCTGTKEG
                     CNEGDCGACTVIVAELAERAASVGTPSADATVVGGLSLRTVNACIQFLPTLDGKALLT
                     VEDLRRIGGGELHPAQQALVECHGSQCGFCTPGIAMTLAST"
BASE COUNT         1166 a         2412 c         2532 g         1070 t
ORIGIN      
        1 cacgaacctc cggttcagca ccctggcgcg cccgcggcgg cggtcgccgc accggctcgc
       61 gggcgtcagt ggccccgccg gccgtcgtac cagagttccg gccggtgggt cggcgttccc
      121 ggcgtcgtga gcgggttgcg cagttcgatg accgtgcgcg tctccaggcg ggccttgcga
      181 aggcgctcgc catagtactg ttggaacaga cgatcgaacg atccgtcggt gatcgcccgt
      241 tcgagcccgc tctcgatgtc gtgcgccagg cgggtgttgc gacgattcac catgaagtac
      301 gcgtcgtaga agtagtgcag cgccaagcgg ggctctacct ccagcgtgct cgcattgctt
      361 tcctgctcgt cccagatctc catcacgctg cggggaaaat agtcgaagcg cttgccgatg
      421 agcatcttga acaggccctc gtaacgggga gccgtgatga catcgatgcc attggcccgg
      481 agaatcgtgg tgtccggcca ctcctgaccc tgcgcgagtc gaacgtgctt caagtccttg
      541 agcgttcgca cgtttgcaaa cgacgactgc tcgcccttgc gaatcaagcc aaggcgccat
      601 ccgaggacgc ccttgttgat gggaatcatg atcggcagca agcgagcctc gcgttcagcc
      661 gaactcggaa tggggccgac gtcgatgctt cgcccggcct cgagctcgag gacctgacgt
      721 tcctggttca tggggaacgc ggcaggctcg atgctgtacg cggtgcccga ctttgacagc
      781 gcgagttgca gcagttgcgt cgcgtagtcg taccggtgat cgccctcgaa ttcaggaact
      841 ggaaatcgaa cgacctcatt ggccagcgcg ctgagcggcg cagacagcac caggcagaag
      901 atccagcgga caacgccgtg gccaaagagg cagctcacgt cgcggcgaag cgcttctgca
      961 gtctgcaacg agcgttccat tgtcttctcc tttggccatc gaaaggctcg ggcgatcgcg
     1021 ccgacggccg aagaccggcg ccgatgcgct ggattgacag cgcaaatact agctcaatcg
     1081 cgggcctgag tcgcctcttc tcccacttcg atgctcgcgc gtcgcaatcg tgggccttca
     1141 atcagtcagt agaaagactc gccaggccag agtgcgctcc gaagggcatg cccgatctca
     1201 ttgggaacgt aacgctcgaa ctgttcgatg aaatgatgaa atcgaatctc gcgctcgatt
     1261 cctgggcgga tgaagaactt gatctggctg acgacgcggg gagaactgcc cacggccgca
     1321 ccggcttcgc tgttcgatgc ggcgctcatg tcggcatctc gaccacggtc cgcaggcgcc
     1381 cggcatcggc atcgcgcgta taagcggtct gcatgtgccg atccgtcgag gcccaaatcg
     1441 aacgaggtcg gccattcgtg gaggccgtcc aggcagggct ggacgaagcc attgatcgcc
     1501 atggcaagtt cgtgtccggc ttcggccacc gattccaccc catcgatcca cgcgcgccgc
     1561 gcttgctgca actggtcgac gaggcttccg cgcagcaggt cgtgaacggc aagttcgcgg
     1621 cgatcgctcg cggcgtggag gccgaactgg ccgggcgcaa gaacggacgc cgaatcccga
     1681 tgaacatcga ccgttgcacc gagctcggtg cgtcggagtt gttcaccggg tcgctcgctc
     1741 ccttggctgg cgcgtttccg tccaagggcg caggacgcgc cctcgatcgc atcgcgtcgg
     1801 ggacatccgg cggcgcgtcg ccagactcag cgtctggcga cgcaatggac tgaatgccga
     1861 ccgggcgaac ggagcgaccg aagcgcagcg ggaagagctc catccagtgc ccagccactt
     1921 ccgggatggc gcgccccatc aggccagtcg cttctcgaag aacacgtcgg gatagggatc
     1981 gtcgttgaag cgcgggatct cgatccagcc cgtggtgcga tagagcgcga gcgcctcggg
     2041 caacgcgctg ttggtatcga gccggagcac gcgcacgccg agaccgcgcg cggcgtcctc
     2101 gacggccgcc atgagacggc gcgccagccc ttgcccgcgc gtcgatgtcg ccacccacag
     2161 ccgcttgacc tccgccgtgt cgccgccgtc gcccttcagg cccacgcagc ccacgggaac
     2221 gccgtccgac caggcaagca ggaagctgcc gcgcggcgcg accatgtcgg cggcgtccgg
     2281 gtcgcgcgag cgcgcgacgt cgaagcccga cgcgaagcgc cgtgccagct cgtcgtaata
     2341 ggcgtgcagg catgcccgcg ccggctcgct gcgcgggtcg gccggctcga tgaccgtgcg
     2401 atcacgctgc aacgccgcgg cgatcacgtc catcgcggcg agcagttttt ccgggtggcg
     2461 atggcgctcg atgaccgcgc cggcccgggc gtccgacagc ttctcgtagg cgcggaactc
     2521 gcgctgcccg gcgcgcgtga gtcgcacgac gcgccgccgc gcgtcgtcgg gatgcgcgac
     2581 ggtctcgacc aggccctcgt cctcgagccc gcgcagcagc cggctggcca gccccgaatc
     2641 gaggcccagc gcttcgcgca ggcgcgcgac gtcgctcacg ccgcggccga tcgagttcag
     2701 gacgcgcgcc gcccccagcg gccggccgcg gccgaggaag gaggtatcga gcgcgcccac
     2761 ctcgacggtg acggcgcggt tgaatcggcg cacgcgggcg atcgcatcgg catccatgta
     2821 tctgacttta gtcagacatg tttgccgcgt caatgccggt ccggacccgg aaatggaaac
     2881 gccgcgcggc tcgcgagaag ccggcgcggc gtcgatccag tcagcgctca gtggccgccg
     2941 acgtagatga accgcaccag gaacaccgcg cagatgatcc acgacatcgg atgcgcctcg
     3001 cgccacttgc cggtgaacag cttgagcgcc gcgtaggtga tgaagccgaa ggccaggccg
     3061 ttggcgatcg agtaggtcag cggcatcgcg agcgccgtca ccaccgcggg cacggactcg
     3121 gtcgtctcgt tccagtcgag gtcgaccagg tcgcgcagca tcaggctggc gacgtacagc
     3181 agcgcgggcg ccgtcgcgta ggccggcacc acgccggcga gcggcgagaa gaacaggcag
     3241 gcgaggaaca gcagggcgac cgccaccgcc gtcaggccgg tgcggccgcc ggcctgcacg
     3301 cccgcggcgc tctcgatgta ggccgtggtg ctggacgtgc ccagcagcga gcccgcgaag
     3361 atggcgctgc tgtcggccag cagggccttg ttcatgcggt gcatcctgcc gtccgtgacg
     3421 aggccggcgc ggcgcgcgac ggccatcagc gtgccggtgg cgtcgaacac ctccaccagg
     3481 aagaacacga ggatgacgtt gaacacgccc acgccaaggg cgcccttgat gtccagctgc
     3541 aggaaggtcg gcgcgatcga gggcggcatc gacacgacgc cgtcgaactt gttgcccgcg
     3601 aagaagaacg acagccccgt cacggccagg atgccgatca ggatggcgcc gcgcacgcgc
     3661 agccggtcca tcaccacgac cgccaggaag ccgatcaccg cgaggatggt cgccggcgcg
     3721 tgcaggtcgc ccagcgtcac gtacgtggct ttcgacggcg cgacgatgcc ggcgctcttg
     3781 agtgcgatga tcgccaggaa catgccgatg cccaccgtga tgccgatgcg cagcgactgc
     3841 gggatgccct ggatgatcag ctctcgaagc ctgaagaccg tggcgagcag gaacaggcag
     3901 cccgagatga agaccgcgcc gagcgcgacc tgccacttca tgcccatgcc cagcaccacc
     3961 acgtaggcga agtaggcgtt caggcccatg cccggcgcga gcgcgatcgg gtagttcgcg
     4021 tacaggccca tgatcagcga gccgagcgcc gcgatcaggc aggtggcgac gaacacggcg
     4081 cccttgggca tccccgcgtc acccaggatg gctgggttga cgaagatgat gtaggccatc
     4141 gtcaggaacg tcgtcaggcc ggccaggagc tcggtgttga cggtggtgcc gtgccgggtg
     4201 aggtcgaacc agcgttcgag ccatgcgggc gcgggcttgg gtggcgtgtg ggcggcggcg
     4261 gactccggcg ccaccgctgc ggtctgggtc gtggaatgca tgttgtctcc tgcatcgctt
     4321 ccaagtggat gcccgggggc gcatctggcg tgcgctggcc tgcgggttcg aaagaccggg
     4381 gctggtttca ctcccggcga tatcgcggca cgccggcgac ataggccgac tccagcaagc
     4441 gctcgtcgcc cagggtcatc caggcaaaga gcttctcgtg caggtcctgc gccaggtcga
     4501 cgcggtgctg catcaggggg gtcgacgccc agtcccacac cgcgatgtcg gcgtggcgac
     4561 cgggctcgag gctgccgatc tcgtggtcca ggtgcagggc gcgcgcggcg ccgcgcgtgg
     4621 cgccgtacag ggccttccac gccgtgagct tgacgccgcg catcgcggcc accttgtagg
     4681 catcggccag cgtgcggatc atcgacaggc tggtgccgcc gccgacatcg ctcgcgaggc
     4741 tgacgcgcgc gccggccgcg ttcatcgcgg tccagtcgaa cagcccgctg ccgaggaaca
     4801 ggttggagct ggggctgtgc gcgatctgtg cgccggtctc gcgcagcagc acgcggtcgg
     4861 tgtcgtccag ccacacgccg tgcgcgagca ccgagcgttc gttcagcagg ccgtggcggt
     4921 ggtagacgtc caggtagctg cgtgcctgcg ggaacagctc ggccacccag cgcacctcgt
     4981 cgcggttctc ggccacgtgc gtctgcatgt aggtgcccgg gtgcgcgcgc cgcagcgccg
     5041 cggccatctc catctgcgcc gacgtgctgg tgggcgcgaa gcgcaccgtc agcgcgtagg
     5101 caaggcggtc gcggccgtgc cagcgctgga tcaggtcgac gcagtcgcgc tcggcgccac
     5161 gcacgtcgtc gcgcagggtg tcgggcgcgt ggcggtccat cagcaccttg ccggcgatga
     5221 tgcgcatgcc gcgccgctgg gcgcccgcga acagcgcatc ggccgaggcc ttgtgcacgg
     5281 tgggaaagac gacggccgac gtggtgccgt gcgccagcag cgcgtccagg aagcgctcgg
     5341 cgcccgcgac ggccgccacc gggtcgccgt gccggctctc ggccggaaag gtgtaggtgt
     5401 tgagccactc cagcagctcg gtgccgtacg aggcgatgac gtccagttgc ggactgtgca
     5461 cgtgcgtgtc gacgaagccg ggcaggatca acttgccgcg gcggtcgacg cgctcccagc
     5521 cgggcgcggg atcctcgggc tgcgcgccga cgatgcggcc gtcctcgacc agcagccaat
     5581 ggtcgggccg gaagcgcacg ccggcggcgc tggcgggtgc ggcaaagccg gggtcgccgg
     5641 tgaagtcgag caggtcgccg cgcagggcga tgcgggaagc ggtggtcatg gggtcggcaa
     5701 gcattgaagt tcagacgagc gactgcgtgg cctcgcggac ctgcgtccgc agccagcggc
     5761 tggcgcccga gtggtggctg agctcgtgcc agagctggta gtagctcagc gaggtcagcc
     5821 gcatgggaca gcgcacgatg cgcacgggca gcgtgtcgac gtagcggctg cagaagcggc
     5881 ggcccgtcgt gagcaccaga tgcgagcgcg ccaccatcag cggcgccagc gcgaagtgcg
     5941 gtgtgcgcac cgtgatgttg cgcgccagcc cctgcccggc gaggtgttcg tcgatcacgc
     6001 ccggcgcgcc ggcgtgcagc ggccgcggcg cgacgtgctc gctctccagg tagcgctcgg
     6061 acgtccacca gcgcgggttg cgcacggcgg ggtggtcggc cgccacgagg cagacgatct
     6121 cgtcggagaa cagctgcccg atgtgcagct cgccgggcgg ctgcagccag ttgccgacga
     6181 ccaggtcgac ctcggcgcgc gacaggtgca gccggtagtc gtattcgtcg gtcagcggca
     6241 ggatctccag cgtcacgccc ggcgcctgcg tcttgatgcg gccgacgacc tcgggcagca
     6301 gcatcgggtc gagccagtcg ctggcggcga tccggaagtg catcgaggcg gtggccgggt
     6361 cgaagtccag gccgcgcgag cgcgagccga acatcagctg ggcgctgcgc agcaggtcgc
     6421 ccgccggctc catcagcgcc agcgccgccg gcgtcggcac catgccgtgg ccggcgcgaa
     6481 cgagcaacgg gtcgcccgtc agctcgcgca gccgcttgag cttggcgctg acggcgggct
     6541 gcgtggtctg cagtcgcagc gcggcccggg aaatgcttcg ttcggtgatc accgtatgga
     6601 atagcctgac gagagatagc tcgatcttgt cgaaagaatt cccctctgcc atacaagaat
     6661 ctccatggtt gctatccagc cggacatata cacccgaagc ccggatcgga gtaactttgg
     6721 tgctgttccc gccacgaatc ccacgccaga ccatgcagac acggccgatc cgcttcttcc
     6781 accaccaccc cggcgagatc gtcgaggtga cggacgccgc acccacgcgc acggtgctga
     6841 actggctgcg cgaggacgcg cgctgcaccg gcacgaagga aggctgcaac gagggcgact
     6901 gcggcgcctg caccgtgatc gtggccgagc tggctgaacg cgcggccagc gtcggcacgc
     6961 cgtcggccga cgccaccgtc gtgggcgggc tgtcgctgcg cacggtcaac gcctgcatcc
     7021 agttcctgcc cacgctggac ggcaaggcgc tgctcacggt cgaggacctg aggcgcatcg
     7081 gcggcggcga gctgcacccg gcgcagcagg cgctggtcga gtgccatggc tcgcagtgcg
     7141 gcttctgcac gcccggcatc gcgatgacgc tggccagcac
//
LOCUS       DATCRO010000938         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1090478, whole genome shotgun sequence.
ACCESSION   DATCRO010000938 DATCRO010000000
VERSION     DATCRO010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090478"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="mreB"
                     /locus_tag="VIN75_25595"
     CDS             <1..583
                     /gene="mreB"
                     /locus_tag="VIN75_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEY8977614.1"
                     /translation="PVSEATGSMVVDIGGGTTEVGVISLGGIVYKGSVRVGGDKFDEA
                     IINYIRRNYGMLIGEQTAENIKMGIGSAFPGSEVREMEVKGRNLSEGVPRSFTISSNE
                     VLEALTDPLNQIVSAVKNALEQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGL
                     PVLVAEDPLTCVVRGCGMALERMERLGAIFTSD"
     gene            723..1649
                     /gene="mreC"
                     /locus_tag="VIN75_25600"
     CDS             723..1649
                     /gene="mreC"
                     /locus_tag="VIN75_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY8977615.1"
                     /translation="MPLGTLDRRPPPLFHQGPSALTRLMVFSAVALFLMAADTRFAVT
                     RPLRSALSILLLPLERALEMPLQALHSSGEYVGGLRQAQADLARSQALLTAQSAKAAR
                     VDTLIAENNRLRALVDLRPALSVKSQAAEVLYEASDPFSRKIFIDRGLSQGVVPGSPV
                     IDEAGVLGQVTRVYPLSSEVTLLIDKEAAIPVLNTRTHQRSAAFGADGAAMELRFMAG
                     NADVKVGDVLQTSGVDGIYPPGLDVARVVKVDPRADSSFARIALAPTASADGVRHVLV
                     LEPMAMQLPPRPAPEPETTPAKGQKANKGIKK"
     gene            1646..2167
                     /gene="mreD"
                     /locus_tag="VIN75_25605"
     CDS             1646..2167
                     /gene="mreD"
                     /locus_tag="VIN75_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY8977616.1"
                     /translation="MIMPRGSDQLLLPVNPLFLWFSLVAALLFNMLPLGRIAAMPDML
                     AVVLVFWNVHQPRRVGIGAAFFFGLLMDVHQGAVLGQHAQAYTLLSFLAITMHRRLLW
                     FSVAEQALQILPVFIVAHAVSLVVRLLSSGSFPGWTLLLAPLFEALLWPVVSWLLLAP
                     QRRAPDRDENRPL"
     gene            2192..>2431
                     /locus_tag="VIN75_25610"
     CDS             2192..>2431
                     /locus_tag="VIN75_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8977617.1"
                     /translation="MTELRNIEVELDRFRTRTIAAAAFVLLCFGVLVFRWFVLQVVRH
                     DDLQAQAEANRIGVVPIVPNRGLIVDRNGIVMATNY"
BASE COUNT          334 a          869 c          841 g          387 t
ORIGIN      
        1 gccggtctcg gaagccaccg gctcgatggt ggtggacatc ggcggcggca ccaccgaggt
       61 gggcgtgatc tcgctgggcg gcatcgtcta caagggcagc gtgcgcgtcg gcggcgacaa
      121 gttcgacgaa gccatcatca actacatccg ccgcaactac ggcatgctca tcggcgagca
      181 gacggccgag aacatcaaga tgggcatcgg ctcggccttc ccgggctccg aggtgcgcga
      241 gatggaggtc aagggccgca acctgtccga aggcgtgccg cgcagcttca ccatctcgtc
      301 caacgaggtg ctggaagcgc tgaccgaccc gctcaaccag atcgtctcgg ccgtcaagaa
      361 cgcgctcgag cagacgccgc cggaactggg tgccgacatc gccgagcgcg gcatgatgct
      421 cacgggcggc ggcgcgctgc tgcgcgacct cgaccgcctg ctggccgagg aaaccggcct
      481 gccggtgctg gtcgccgagg atccgctgac ctgcgtcgtg cgcggctgcg gcatggcgct
      541 ggagcgcatg gaacgcctgg gcgcgatctt cacttcggac tgagcgacgc atcccggggc
      601 ggcgtccccg ggtgcgggtt ccttcgcaac ggatgaggaa ctcgctgcgg cgataattca
      661 tctgaccgca accttcaccc tcctgtcctc cgccgcaccc cgcgcggcgc gctgagtacc
      721 ccatgccgct cggtaccctg gaccgccgtc caccgccgtt gttccaccag ggcccgtccg
      781 cgttgacgcg cctgatggtg ttctccgcgg tggcgctgtt cctgatggcg gccgacaccc
      841 gcttcgcggt cacgcgcccg ctgcgctcgg cgctgtcgat cctgctgctg ccgctggagc
      901 gtgcgctcga gatgcccctg caggcgctgc attccagcgg cgagtacgtc ggcggcctgc
      961 gccaggcgca ggccgacctc gccaggtcgc aggcgctgct gacggcgcag tcggccaagg
     1021 cggcgcgcgt ggacaccctc atcgccgaga acaaccgcct gcgcgcgctg gtcgacctgc
     1081 ggccggcgct gagcgtgaag tcgcaggccg ccgaggtgct gtacgaggcg agcgacccgt
     1141 tctcgcgcaa gatcttcatc gaccgcggcc tctcgcaggg cgtggtgccg ggctcgccgg
     1201 tgatcgacga ggccggcgtg ctgggccagg tcacgcgcgt gtacccgctg tcgtccgagg
     1261 tgacgctgct gatcgacaag gaagccgcga tccccgtgct caacacccgc acccaccagc
     1321 gcagcgcggc cttcggcgcc gacggcgccg cgatggagct gcgcttcatg gccggcaacg
     1381 ccgacgtcaa ggtgggcgac gtgctgcaga ccagcggcgt cgacggcatc tacccgccgg
     1441 ggctggacgt cgcgcgcgtc gtcaaggtcg acccgcgcgc cgactcgtcg ttcgcccgca
     1501 tcgcgctggc gcccaccgcg tcggccgacg gcgtgcgcca cgtgctggtg ctcgagccga
     1561 tggcgatgca gctgccgccg cgtccggcgc ccgagcccga gaccacgccg gccaagggcc
     1621 agaaggcgaa caaggggatc aagaagtgat catgccgcgc ggctccgacc agctgctgct
     1681 gccggtcaac ccgctgttcc tctggttctc gctcgtcgcc gcgctgctgt tcaacatgct
     1741 gccgctcggg cgcatcgcgg cgatgccgga catgctggcc gtcgtgctcg tgttctggaa
     1801 cgtgcaccag ccgcgccgcg tgggcatcgg cgccgcgttc ttcttcggcc tgctgatgga
     1861 cgtgcaccag ggcgccgtgc tgggccagca cgcgcaggcc tacacgctgc tgagcttcct
     1921 ggccatcacg atgcaccgcc gcctgctgtg gttcagcgtg gccgagcagg cgctgcagat
     1981 cctgccggtg ttcatcgtgg cgcacgcggt gtcgctggtg gtgcggctgc tgtcgtccgg
     2041 cagcttcccc ggctggacgc tgctgctggc gccgctgttc gaggcgctgc tgtggccggt
     2101 ggtgtcgtgg ctgctgctgg cgccgcaacg ccgcgctccg gaccgcgacg agaaccgtcc
     2161 cctctgatcg agcgagcccc acgtgaagtc gatgaccgaa ctgcggaaca tcgaggtcga
     2221 actcgatcgc ttccgcacgc gcacgatcgc ggcggcggcc ttcgtgctgc tgtgcttcgg
     2281 cgtgctcgtc ttccgctggt tcgtgctgca ggtggtgcgc cacgacgacc tgcaggcgca
     2341 ggccgaggcc aaccgcatcg gcgtcgtgcc catcgtgccc aaccgcggcc tgatcgtcga
     2401 ccgcaacggc atcgtgatgg ccaccaacta c
//
LOCUS       DATCRO010000939         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_33365, whole genome shotgun sequence.
ACCESSION   DATCRO010000939 DATCRO010000000
VERSION     DATCRO010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33365"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIN75_25615"
     CDS             complement(<1..286)
                     /locus_tag="VIN75_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8977618.1"
                     /translation="MQIHRGLASLATLALARGGTALTIGNFDGVHRGHQAMLALLRSE
                     ARHRGVPSCVLSFEPHPRDFFARRTGQPQLAPARIANLRDKLTELERCGID"
     gene            651..2522
                     /locus_tag="VIN75_25620"
     CDS             651..2522
                     /locus_tag="VIN75_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEY8977619.1"
                     /translation="MRFLPSRRLRASALAASLAAAFSGSAFALNPSDTSVQMFEWGWN
                     DLATECTQFLGPQGFGAIQISPPQASKVTSTWWGVYQPVNYVNLTGKFGTQAQLQAMI
                     NTCHAAGVRVYADIVVNQMAADSGTATDGSTWNSSTLTYPYFSSNDFHAACDIQSSDY
                     NSPAGRSNVQTCRLSGMPDLNSESSYVQGQVVNYMNALLTLGIDGFRIDAAKHQAASS
                     LQAIMNSVKSTHPTTNQGEAIWVTQEIVPDGEVSRSDYFPIGTINEFQFTYAMRDAFR
                     NANGSSPSTIPTMMGTWNNWGGTWGFVQPANATVFINNWDTERNGGSLDASNYTGATN
                     DTQGSHRYDLANIFMLAQGYGEAQLQSGFRFSSNDQDRPSASPYSNGVAQINVAWDFV
                     HRWGDIYPMVKFRSAARNQAQTNWVTGNANQIAFSRGNVGFVALNNSTSAWSQTFYTG
                     LPAGTYCNIVHGLANAGATACTSDSVTVNASGYATITVPADGGSTVPAVAIYTGQTVS
                     GSGGGGGSTCSVTFTIANANTTFGQNLYVVGNQAALGNWTPASGFALAIQGSGANVPW
                     TGTISLPANTAMQYKYVKWNGSTAVWESNQSTSSGNREFTSCAAGATVSRNDGNFKN"
     gene            complement(2776..4605)
                     /locus_tag="VIN75_25625"
     CDS             complement(2776..4605)
                     /locus_tag="VIN75_25625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977620.1"
                     /translation="MGNPSSSIPAPVADARAAAAAIDRQIGFERITTLYQITEPMLFG
                     GFGFTVLTGLLLASSVSQALLAAWVGFKIALTIARLVDGRLFLRDRHRSLRLRVWKIR
                     FFVGVILDSVAWGLIPVLFMPTSDPAVNGVLIAGMVGIASAGTIALSAHRLASGTSLL
                     LVMGPLIEQQAMTPTPLARYTAAAAVVYAALLFLEARNSHRRFTETSRLRFENAAIAE
                     ERRRALVFAEHSNAAKTRFLASVSHELRTPLNGIIGMTQLVAVEPLSALQRQRLDVLR
                     HSAEHLVTVIGDLLDLSRIEFDRIELNPQPTLIAQTVRDVTDLLQPVAEERGLAFEVV
                     FDLGLPAAALMDASRVKQVLHNLIGNALKFTAHGGVGVAVAMADGHLCFRVSDSGEGI
                     PPDAIERIFDAFEQGPSSTAPARSGTGLGLTISRRLARAMGGDVTCRSVPGRGTTFEF
                     TIAFRPTPRTPTPRPSLSEELAAAGESADPPAVRPGRVLVVEDNEVNALVVRGMLEQM
                     GVNAELAVDGQKALARMGQIAYDLVLMDCQMPVLDGWEATRIWRARETRLRQNQRLPI
                     VALTASAAAGERERCLEAGMDDYLSKPFTREALGELVDRYLAA"
     gene            complement(4713..5852)
                     /locus_tag="VIN75_25630"
     CDS             complement(4713..5852)
                     /locus_tag="VIN75_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8977621.1"
                     /translation="MDFDFSDDEQQLSEAVQRFVERGYGFERRTAIAKAGGFSAQAWG
                     EIAELGILGLGTAEADGGMAMGPVAPMLALQALGRGLVLEPVAAVALVAASVLGQADA
                     QARASWSPRVASGEARVVLAYQERAARYRLERVDTQAARNGDGWTLSGSKSLVPIGDQ
                     ADAFIVSARTGGAADDPAGIALFLVERAATGVSTRGYALQDGSRAAEVMLADAPATLL
                     LPAGRAFAALEQAIDIGIAALAAEAVGVMDKLFDLTVDYLNTRKQFGVAIGTFQALRH
                     RIADMKMQLELARSMSYYATLKLGEAPDVRRRAAAQAKVQLGNSARFLGQQAIQLHGG
                     IGMTDEYIGSHYFKRLTTMEMQFGDTMHHIGEVSARMTETAGAFA"
BASE COUNT          986 a         2110 c         2063 g          898 t
ORIGIN      
        1 ggtcgatgcc gcagcgctcc agctcggtga gcttgtcgcg caggttggcg atgcgtgccg
       61 gcgccagttg cggctgcccc gtgcggcgcg cgaagaagtc gcgcggatgc ggctcgaagc
      121 tcagcacgca actgggcacg ccgcgatggc gcgcctcgct gcgcagcagc gccagcatcg
      181 cctggtggcc gcggtgcacg ccgtcgaagt tgccgatggt cagcgccgtt ccgccgcgcg
      241 cgagggcaag ggtggcgagg gaggcgaggc cgcggtggat ctgcatggcg ggattatcga
      301 ccgccgcgct ggcggccccg aacgcgtggg gtcgatgcga cgatggcctc aagcatggta
      361 ctgaaactac aaatcaatag ttttacttgg agtcgatatt gtgtaattac tttttattga
      421 gacaggaatg tatgcggaaa aataagaggt aaaagagggc cgcgtatacc ctggatcagg
      481 gagttcatcc tcttgtttcg gcacactctg ggtattaaaa ctacttcaaa ccagagaaag
      541 gacacggccc ggctcccaca gagcgcctcc cccgcgcagc gcgcggacct cgtgacgatc
      601 aacaagacca aggcgaccgg cacggatcgg gacgcccgga gacaaccacc atgcgattcc
      661 tcccttcccg ccggctgcgc gcgagcgcgc tggccgcctc cctggccgcg gccttcagcg
      721 gcagcgcctt cgcgctcaac cccagcgaca cctcggtgca gatgttcgag tggggctgga
      781 acgacctggc caccgagtgc acccagttcc tcggtccgca gggcttcggc gcgatccaga
      841 tctcgccgcc gcaggcgtcc aaggtcacca gcacctggtg gggcgtctac cagccggtga
      901 actacgtcaa cctgaccggc aagttcggca cgcaggcgca gctgcaggcg atgatcaaca
      961 cctgccacgc cgcgggcgtg cgcgtgtacg ccgacatcgt cgtcaaccag atggccgccg
     1021 actccggcac cgccaccgac ggctcgacct ggaattcgtc gacgctcacc tatccgtact
     1081 tcagctcgaa cgacttccac gccgcctgcg acatccagtc ctccgactac aactcgccgg
     1141 ccgggcgctc gaacgttcag acctgcaggc tgtcgggcat gcccgacctg aactcggaaa
     1201 gctcgtacgt gcaggggcag gtcgtcaact acatgaacgc gctgctgacg ctgggcatcg
     1261 acggcttccg catcgacgcc gccaagcacc aggccgcgtc gtcgctgcag gcgatcatga
     1321 actcggtcaa gtcgacgcac ccgaccacca accagggcga ggcgatctgg gtgacgcagg
     1381 agatcgtgcc cgatggcgag gtgtcgcgct ccgactactt cccgatcggc accatcaacg
     1441 agttccagtt cacctacgcg atgcgcgacg cgttccgcaa cgccaacggc tccagcccgt
     1501 cgacgatccc gacgatgatg ggcacctgga acaactgggg cggcacctgg ggcttcgtgc
     1561 agcccgccaa cgccacggtg ttcatcaaca actgggacac cgagcgcaac ggcggctcgc
     1621 tggacgccag caactacacc ggcgccacca acgacacgca gggctcgcac cgctacgacc
     1681 tggccaacat cttcatgctg gcgcagggct acggcgaggc gcagctgcag tcgggcttcc
     1741 gcttctccag caacgatcag gatcgcccga gcgccagccc gtactcgaac ggcgtcgcgc
     1801 agatcaacgt ggcctgggac ttcgtgcacc gctggggcga catctacccg atggtcaagt
     1861 tccgctccgc ggcgcgcaac caggcgcaga cgaactgggt cacgggcaac gccaaccaga
     1921 tcgcgttcag ccgcggcaac gtcggcttcg tggcattgaa caacagcacg tcggcgtggt
     1981 cgcagacctt ctacacgggc ttgccggccg gcacgtactg caacatcgtg cacggcctcg
     2041 cgaacgcggg cgcgacggcc tgcaccagcg attcggtgac ggtcaacgcc agcggctacg
     2101 ccacgatcac cgtgccggcc gacggcggtt ccacggtgcc ggcggtggcc atctacacgg
     2161 ggcagacggt gtcgggttcc ggtggtggcg gcggctcgac gtgctcggtg accttcacca
     2221 tcgccaacgc gaacaccacc tttggccaga acctgtacgt ggtgggcaac caggctgcgc
     2281 tcggcaactg gacgccggcc tcgggcttcg cgctggcgat ccagggttcc ggcgcgaacg
     2341 tgccctggac cggaactatt tccttgccgg ccaatacggc gatgcaatac aagtacgtga
     2401 agtggaacgg ttccacggcc gtctgggaaa gcaaccagtc gacctcgagc ggcaatcgcg
     2461 agttcacctc ctgcgcggca ggcgccacgg tgtctcgcaa cgatggcaac ttcaagaact
     2521 gacgtcggaa gaacgtcgtt gggtgaaggc tggcgggcga gagtccgccg gccttttttc
     2581 ttggaggggt ctggcctccc ctcgaatcgc gccgatgcat cgatcccagc cacgggactg
     2641 gtcgcgattc gaggcgaagg cctgaccccg atcgctcgcg atgatcgggg gtcaggcctt
     2701 cgccttcgcc cgcgaatcgg aagcgtccct acgatcggct ggaccccgcg gacgaaggag
     2761 aggccagacc ccgtctcaag ccgccagata ccgatccacg agctccccca gcgcctcgcg
     2821 cgtgaacggc ttgctcaggt agtcatccat ccccgcctcc aggcagcgtt cgcgctcgcc
     2881 ggcggcggcg ctggcggtca gcgccacgat gggcaggcgc tggttctggc gcaggcgcgt
     2941 ctcgcgggcg cgccagatgc gcgtggcctc ccagccgtcg agcacgggca tctggcaatc
     3001 catcagcacc aggtcgtagg cgatctgccc catgcgcgcg agcgccttct gtccgtcgac
     3061 ggccagttcc gcgttgacgc ccatctgctc gagcatgccg cgcaccacga gcgcgttgac
     3121 ctcgttgtcc tcgacgacca gcacgcggcc ggggcgcacc gccggcgggt ccgccgactc
     3181 gccggccgcg gccagctcct cgctcagcga cggccgcggc gtgggcgtgc gcggcgtcgg
     3241 gcggaaggcg atggtgaact cgaaggtggt gccgcgcccg gggacggacc ggcacgtgac
     3301 gtcgccgccc atcgcgcgcg ccagccggcg cgagatcgtc aggcccaggc cggtgcccga
     3361 gcgcgccggc gccgtgctgg acgggccttg ctcgaaggcg tcgaagatgc gctcgatcgc
     3421 gtcgggcggg atgccttcgc ccgagtcgct gacgcggaag cacaggtgcc cgtcggccat
     3481 ggccaccgcc acgcccacgc cgccgtgcgc cgtgaacttc agcgcgttgc cgatcaggtt
     3541 gtgcagcacc tgcttgacgc gcgaggcgtc catcagcgcc gcggcgggca ggcccaggtc
     3601 gaacaccacc tcgaaggcca gcccgcgctc ctcggccacc ggttgcagca ggtcggtgac
     3661 gtcgcgtacc gtctgcgcga tcagcgtggg ctgcgggttg agctcgatgc ggtcgaactc
     3721 gatgcgcgac agatcgagca ggtcgccgat gaccgtgacc aggtgctccg cggaatggcg
     3781 cagcacgtcc agccgctggc gctgcagcgc ggacagcggc tccaccgcca ccagttgcgt
     3841 catgccgatg atgccgttca gcggcgtgcg cagctcgtgg ctgaccgacg cgagaaagcg
     3901 cgtcttggcc gcgttggagt gctcggcgaa caccagcgcg cggcgccgtt cctcggcgat
     3961 ggcggcgttc tcgaagcgca gccgcgacgt ctcggtgaag cggcggtggc tgttgcgtgc
     4021 ctcgaggaac agcaacgcgg cgtagacgac cgccgcggcg gcggtgtagc gggcgagggg
     4081 cgtgggcgtc atcgcctgct gctcgatcag cgggcccatc accagcagca gcgacgtgcc
     4141 ggacgccagg cggtgcgcgg acagcgcgat cgtgccggcc gaggcgatgc ccaccatgcc
     4201 cgcgatcagc acgccgttga cggccgggtc gctggtgggc atgaacagca ccgggatcag
     4261 gccccaggcc accgaatcga ggatgacgcc gacgaagaag cggatcttcc acacgcgcag
     4321 ccgcagcgag cgatggcggt cgcgcaggaa caggcggccg tcgaccaggc gcgcgatcgt
     4381 cagcgcgatc ttgaagccga cccacgcggc cagcagcgcc tgggacacgc tggaggcgag
     4441 cagcagcccc gtcagcaccg tgaagccgaa gccgccgaac agcatcggct cggtgatctg
     4501 gtagagcgtg gtgatgcgct cgaagccgat ctgccggtcg atcgcggccg ccgcggcgcg
     4561 cgcatcggcc accggcgcgg gaatcgagct ggatggattc cccaccgggg cttccgtcag
     4621 gcaacggcgg cgatcatgat tcgccgcagt ctaccgagtt tccggccggc gcggccctga
     4681 catcgtgtga agacgacggg actgcgggtg cgtcaggcga acgcgcccgc ggtctcggtc
     4741 atgcgggcgc tcacctcacc gatgtggtgc atcgtgtcgc cgaactgcat ctccatcgtc
     4801 gtcaggcgct tgaagtagtg gctgccgatg tactcgtcgg tcatgccgat gccgccatgc
     4861 agctggatcg cctgctggcc caggaagcgc gcgctgttgc ccagctgcac cttggcctgg
     4921 gcggcggcgc ggcggcgcac gtcgggcgcc tcgcccagct tcagcgtggc gtagtagctc
     4981 atcgagcgcg ccagctccag ctgcatcttc atgtccgcga tgcgatggcg cagcgcctgg
     5041 aaggtgccga tcgccacgcc gaactgcttg cgggtgttca ggtagtcgac ggtcaggtcg
     5101 aacagcttgt ccatcacgcc cacggcttcg gcggccaggg cggcgatgcc gatgtcgatg
     5161 gcctgttcga gcgccgcgaa cgcgcggccg gccggcagca gcagcgtggc gggcgcgtcg
     5221 gccagcatga cctcggcggc gcgggagccg tcctgcagcg cgtagccgcg cgtgctcacg
     5281 ccggtcgccg cgcgttcgac gaggaacagc gcgatgccgg ccggatcgtc cgcggcgccg
     5341 ccggtgcggg cgctgacgat gaaggcgtcc gcctggtcgc cgatcggcac caggctcttg
     5401 ctgccggaca gcgtccagcc gtcgccgttc ctggccgcct gggtatcgac ccgctccagg
     5461 cgatagcgcg cggcacgctc ctggtaggcg aggacgaccc gcgcctcgcc cgacgcgacg
     5521 cgcggcgacc agctcgcgcg cgcctgcgcg tccgcctgcc cgagcaccga cgcggccacc
     5581 agcgccaccg ccgccaccgg ctccagcacc aggccgcggc ccagcgcctg cagcgccagc
     5641 atcggcgcga ccgggcccat ggccatgccg ccgtccgcct ccgcggtgcc caggcccagg
     5701 atgcccagct cggcgatctc gccccacgcc tgcgcgctga agccgccggc cttggcgatg
     5761 gcggtgcggc gctcgaagcc gtagccgcgc tcgacgaagc gctgcacggc ctcgctcagt
     5821 tgctgttcgt cgtcgctgaa atcgaaatcc atcattgatc ctttcggcct gggccgctga
     5881 ctacgactcg ggcgagcgcg cggccgctcc ggagcgagcc cgacagcccc gcgggggcga
     5941 cgagcgaagc gagtggggtc gtcatacgcc gaggaccgtc tgagcgacga tattgcgttg
     6001 cacttcgttg gaaccgccgt agatcgtcgt cttgcggtag ttgaagtagt tggccgc
//
LOCUS       DATCRO010000940         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_449345, whole genome shotgun sequence.
ACCESSION   DATCRO010000940 DATCRO010000000
VERSION     DATCRO010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449345"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VIN75_25635"
     CDS             complement(<1..1173)
                     /locus_tag="VIN75_25635"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8977622.1"
                     /translation="MKKLILAACVAASFVAGGAHAAQGRVPEGIPSLDHVFVIMMENH
                     AYAQVAGNPQAPFINAMMARANVAKNYYAIAHPSLTNYLEVVGGSNFNNLSDQYPDWH
                     DTACIPSIAPGRATNTDSPDTGAICPIAGHHGTDAPTPALDMTMNETSAPPLTNIDGM
                     RAIPASNDIDGITIADQLVRAGKTWKSYQEGLPEIGADGVNVSDGFYSIGGKNAKGVS
                     VTSDFAALSTAGNPVSAANVVYLYAVKHNPFAYFASVQEGWNPELSMHRMAGFDGENG
                     LYADLKSGHVPNFAFIAPNQCEDMHGRGNGTAFCNYDADDNGTQVGLNPSLILQGDLA
                     VQRIVTAIKASRTWAERRSAIVILWDENDYSTRTSNQVVTIVDTNYGQHHVASTKFY"
     gene            1419..3524
                     /locus_tag="VIN75_25640"
     CDS             1419..3524
                     /locus_tag="VIN75_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEY8977623.1"
                     /translation="MTHSNRRNFLRLAASAGVVGANAVPGLIEKALAIPAHRETGTIK
                     DVKHIVILMQENRSFDHYFGAMKGVRGFGDPRPATLPNGKSVWHQPNNGGELLPFHPD
                     APNLGLQFLDGTPHDWSTTHAAWNGGRYDQWIAAKGTNTMAYFTRQDIPFHYALADAF
                     TVCDAYHCALLGPTDPNRYHMWTGWVGNDGSGGGPIVDNAEAGYGWSTYPERLQAAGI
                     SWKIYQDVGNGLTAAGFWGWTDNPYIGNYGDNSLLYFHQYQNALPGTPLYDNARTGTN
                     VLADPTQTFFDILKADVAKGTLPQVSYIVAPEAFSEHPNWAPNFGAWYTSQVLDILTS
                     NEELWSQTALFITYDENDGFFDHVVPPTPAQSPAQGQSTVTTANDFFPGNASYPAGPF
                     GMGQRVPMIVVSPWTKGGWVCSEVFDHTSLIRFIEQRFGQGRPGLRETNITPWRRAVA
                     GDLTSAFNFASPNDAVPALPDTAGYVPQDALRHDSYKPVPPSVQVMPTQERGLKWSRA
                     LPYNLAVHGRIDAAHRTFALDFANRGDAGAVLRVSVAGSADAPRTYTVEPGRKLHDAW
                     STVPANGRYAFTVTGPGAFHREFAGDAAAASGRASIAPEARIEHVGFGDDCVRLLLSN
                     EGTTAVTLTVAANAYSRAPARHIRLAPDQVVEERWPVADTYGWYDLSVTCDASPAFLR
                     RAAGRVENGRPRVSDPATA"
     gene            3659..>4450
                     /locus_tag="VIN75_25645"
     CDS             3659..>4450
                     /locus_tag="VIN75_25645"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY8977624.1"
                     /translation="MFAGGRTGAAGLALAAGLLATAAARAADYRIGPTPAWVRPVPLA
                     ALPHPQADAANVAYGLRYDLIDDQVRLEAGARGRYHHEISEAVTDKGIDALSHHEIAF
                     DPSWEKLTLVQLDVIRDGKRVSRLREVQVKVLQRERDLESRIYDGRKSVMLDLPDVRV
                     GDIVEFAYARAGMNPVFQGHHASDFDMQWEVPVARVHRRLSTAAGLGVHVASLSGAPA
                     PQVSQSEGFDERTWDLHDVPALRMEASVPGSYNPYPWIEWSDFASW"
BASE COUNT          660 a         1561 c         1551 g          678 t
ORIGIN      
        1 gtagaacttc gtgctcgcca cgtggtgctg gccatagttg gtgtcgacga tcgtcacgac
       61 ctggttggac gtgcgcgtgc tgtagtcgtt ctcgtcccaa aggatgacga tcgcgctgcg
      121 gcgctcggcc caggtgcgcg aggccttgat cgccgtgacg atgcgctgca cggccaggtc
      181 gccctgcagg atcagcgacg gattcaggcc gacctgcgtg ccgttgtcgt cggcgtcgta
      241 gttgcagaac gcggtgccgt tgccgcggcc gtgcatgtcc tcgcactggt tcggcgcgat
      301 gaacgcgaag ttgggcacgt ggccggactt caggtcggcg tacaggccgt tctcgccgtc
      361 gaagccggcc atgcggtgca tgctcagttc cgggttccag ccttcctgca cggacgcgaa
      421 gtaggcgaac gggttgtgct tgacggcgta caggtagacg acgttggcgg ccgacaccgg
      481 gttgccggcg gtcgacaggg cggcgaaatc cgacgtgacg ctcacgccct tggcgttctt
      541 gccgccgatg ctgtagaagc cgtcgctgac gttgacgccg tcggcgccga tctcgggcag
      601 gccttcctgg tagctcttcc aggtcttgcc ggcgcgcacc agctggtcgg cgatggtgat
      661 gccgtcgatg tcgttgctgg cggggatggc gcgcatgccg tcgatgttgg tcagcggcgg
      721 ggccgacgtc tcgttcatcg tcatgtcgag cgcaggcgtg ggcgcgtcgg tgccgtggtg
      781 gccggcgatg gggcagatcg cgccggtgtc ggggctgtcg gtgttggtgg cgcgtcccgg
      841 ggcgatgctc gggatgcagg ccgtgtcgtg ccagtcggga tactggtcgg acaggttgtt
      901 gaagttggag ccgccgacca cttcgaggta gttcgtcagg ctcgggtgcg cgatcgcgta
      961 gtagttcttg gcgacattgg ccctggccat catcgcgttg atgaacggcg cctgcgggtt
     1021 gccggcgacc tgcgcgtacg cgtggttctc catcatgatc acgaagacgt ggtcgagcga
     1081 cgggatgccc tcgggcacgc ggccctgcgc ggcatgcgcg ccgcccgcga cgaacgacgc
     1141 cgcgacgcag gcggcgagga tgagtttctt catggctgtc tggctcctgg cttgacttgt
     1201 tgtagcgggg cggccccggg gaatgcgcga ccgtcgaccg gccacgatgg tgcgcagtcg
     1261 acatgacgga tccgtgtcga cggccggcgg gtcacgcagc ggaaagcttg ctgcaaggaa
     1321 cctgggggca tcgggcgcgt gtcaccgcgc tgacacggcg gcttcctagc gtgcagggct
     1381 ttcgcagcaa cccatcgccc aagcgccagg agcccccgat gacccattcg aatcgccgca
     1441 acttcctccg cctcgccgcc tcggccggtg tcgtcggcgc caatgccgtg cccggcctga
     1501 tcgagaaggc cctggccatc cccgcgcacc gcgagacggg cacgatcaag gacgtcaagc
     1561 acatcgtcat cctgatgcag gagaaccgct cgttcgacca ctacttcggc gcgatgaagg
     1621 gcgtgcgcgg cttcggcgat ccgcggccgg cgaccctgcc caacggcaag agcgtgtggc
     1681 accagccgaa caacggcggc gaactgctgc cgttccatcc cgacgcgccc aacctcggcc
     1741 tgcagttcct cgacggcacg ccgcacgact ggtccaccac gcacgcggcc tggaacggcg
     1801 gccgctacga ccagtggatc gcggccaagg gcaccaacac gatggcctac ttcacgcgcc
     1861 aggacatccc gttccactac gcgctggccg acgccttcac cgtgtgcgac gcctaccact
     1921 gcgcgctgct gggcccgacc gacccgaacc gctaccacat gtggacgggc tgggtcggca
     1981 acgacggctc gggcggcggc cccatcgtcg acaacgccga agccggctac ggctggtcga
     2041 cctaccccga gcgcctgcag gccgcgggca tcagctggaa gatctaccag gacgtgggca
     2101 acggcctgac cgccgccggc ttctggggct ggaccgacaa tccgtacatc ggcaactacg
     2161 gcgacaactc gctgctgtac ttccaccagt accagaacgc gctgcccggc acgccgctgt
     2221 acgacaacgc gcgcaccggc accaacgtgc tggccgaccc gacgcagacc ttcttcgaca
     2281 tcctcaaggc cgacgtcgcc aagggcacgc tgccgcaggt ctcgtacatc gtggcgcccg
     2341 aggccttcag cgagcacccg aactgggccc cgaacttcgg cgcctggtac acgtcgcagg
     2401 tgctggacat cctgacctcc aacgaagagc tgtggagcca gacggcgctg ttcatcacct
     2461 acgacgagaa cgacggcttc ttcgaccacg tcgtgccgcc cacgcccgcg cagtcgcccg
     2521 cgcagggcca gtcgacggtg accacggcca acgacttctt tcccggcaac gcgagctacc
     2581 ccgccggccc gttcggcatg ggccagcgcg tgccgatgat cgtcgtctcg ccgtggacca
     2641 agggcggctg ggtgtgctcc gaggtgttcg accacacctc gctgatccgc ttcatcgagc
     2701 agcgcttcgg ccagggcagg ccgggcctgc gcgagaccaa catcacgccg tggcgccgcg
     2761 ccgtcgccgg cgacctgacc tcggccttca acttcgccag ccccaacgac gccgtgcccg
     2821 cgctgcccga cacggccggc tacgtgccgc aggacgcgct gcgccatgac agctacaagc
     2881 cggtcccgcc gtcggtgcag gtgatgccca cgcaggagcg cggcctgaag tggtcgcgcg
     2941 cgctgccgta caacctcgcc gtgcatggcc gcatcgacgc cgcgcatcgc acgttcgcgc
     3001 tggacttcgc caaccgcggc gacgccggcg cggtgctgcg cgtcagcgtg gccggcagcg
     3061 ccgacgcgcc gcgcacctac accgtcgagc cgggcaggaa gctgcacgac gcgtggtcga
     3121 cggtgcccgc caacggccgc tacgccttca cggtgaccgg gccgggcgcg ttccaccgcg
     3181 agttcgcggg cgacgccgcc gccgcgtcgg gccgcgccag catcgcgccg gaagcgcgca
     3241 tcgagcacgt cggcttcggc gacgactgcg tgcgcctgct cttgtccaac gaaggcacga
     3301 ccgccgtcac gctgacggtg gccgccaacg cctactcgcg tgcgccggcg cgccacatcc
     3361 gcctcgcgcc ggaccaggtg gtcgaggaac gctggccggt cgcggatacc tacggctggt
     3421 acgacctgtc ggtgacctgc gacgccagcc cggcgttcct gcgccgcgcg gcgggccggg
     3481 tcgagaacgg ccgtccgcgc gtgagcgatc cggcgaccgc ctgaggccgt tgcaactggc
     3541 gcgcccaccc tttttgggga cgggcgcgcc ccgatcgatc gcgcccttgc gcgcggcgaa
     3601 aatcgaggct cggacgctcg accaacgagc gcgctttcga gtcgcaaggg agacggacat
     3661 gttcgcaggt gggcgcacgg gcgcggcagg gctggcgctc gcggcgggcc tgctggccac
     3721 cgccgcggca cgggccgccg actaccgcat cgggccgacg cccgcatggg tgcggccggt
     3781 gccgctcgcg gcgctgccgc acccgcaggc cgacgcggcc aacgtcgcct acggcctgcg
     3841 ctacgacctc atcgacgacc aggtgcggct cgaggcgggc gcccgcggcc ggtaccacca
     3901 cgagatcagc gaggccgtca ccgacaaggg catcgacgcg ctgagccacc acgagatcgc
     3961 gttcgacccg tcctgggaga agttgacgct ggtgcaactg gacgtcatcc gcgacggcaa
     4021 gcgcgtctcg cgcctgcgcg aggtgcaggt gaaggtgctg cagcgcgagc gcgacctcga
     4081 gtcgcgcatc tacgacgggc gcaagtcggt gatgctggac ctgcctgacg ttcgcgtcgg
     4141 cgacatcgtc gagttcgcct atgcgcgcgc cggcatgaac cccgtgttcc agggccacca
     4201 cgccagcgac ttcgacatgc agtgggaagt gccggtggcg cgcgtgcacc ggcggctgtc
     4261 gacggcggcg ggcctgggcg tgcacgtcgc gtccttgagc ggcgcgccgg cgccgcaggt
     4321 gtcgcagtcc gaaggcttcg acgagcgcac ctgggacctg cacgacgtgc ccgcgctgcg
     4381 catggaggcg tcggtgccgg gctcgtacaa cccgtacccg tggatcgagt ggagcgactt
     4441 cgcgtcgtgg
//
LOCUS       DATCRO010000941         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_168324, whole genome shotgun sequence.
ACCESSION   DATCRO010000941 DATCRO010000000
VERSION     DATCRO010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168324"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VIN75_25650"
     CDS             <1..996
                     /locus_tag="VIN75_25650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977625.1"
                     /translation="GVGLFKGNIAAQVGALYRPGDKRNASAFQIFLLAVQVAVIVSPL
                     VCGTLGEKVAWHWGFDAAGVGMLIGLITYLSGRGWLPPEPERKSASATIEQPKITHRE
                     WGRVAILVGIIPILMLTILGNQQISTGFPLWSDKHMDLVMFGWRVPTTWLQVVDAAVS
                     FVTMLLSIAFWKWWSLRRTEPDELVKMGIGTLIAAAGPGAMMIAGGIVDATHARVGFG
                     WTLAYTICNDLGFANILPVGLALYSRVAPKQIVGLVVGIYYLHLSIGNTYVGWLAGLM
                     EKMPSAQFWGMHAALVALGGGLLLVVRMLFWRWLSPAAILPAGQEEPDAALAPAH"
     gene            1085..2071
                     /locus_tag="VIN75_25655"
     CDS             1085..2071
                     /locus_tag="VIN75_25655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY8977626.1"
                     /translation="MQTPPPLMHAVLIRRFGGPEVVELADVPVPVPSDGELLLKVEAA
                     SLNPVDWKIRSGKYPVVKDDKLPFVLGRDFSATVLQSDDAALPAGTLVHGMADMAHGA
                     LAQYTVARPGQLAAVPGTLDRMAAAAVPLAALTAWQGLFDQGGLRAGQRVLIHAGAGG
                     VGHFAVQFAKARGAHVITTVSTDDVELARSLGADEVIDHLAERFETKTRDIDLVFDLV
                     GGAVQEHSWAVLKRGGTLVSTVGQPPQDKAEAIGVRALGYQARPDADQLREIDALIQS
                     GKVRPIVSRKYAVGEFKEALRTLEDGHVTGKLVVDLAGAVADVPDPIYRPGA"
     gene            complement(2147..>4120)
                     /locus_tag="VIN75_25660"
     CDS             complement(2147..>4120)
                     /locus_tag="VIN75_25660"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8977627.1"
                     /translation="ALMHDTIEDCGVTKPELIERFGAPTADLVDGLTKLDKLQFSTRE
                     DGQAESFRKMLLAMSRDVRVILIKLCDRLHNMRTMDAMAPAKRARIARETIEIFAPIA
                     HRLGLNQMYRELQDLSFRFMYPWRYAAIAKALERARGTRRDIVDRMRRDVEKAFAEAR
                     SKVTVFSREKTVFSIYRKMREKHAGFAQVNDIFGFRIVVHSLPECYLSLGTLHQMYKP
                     VPGRFKDYIAIPKANGYQSLHTTLVNPLGTAVEFQVRTEEMHAVAEAGIAAHWMYKAG
                     PQGGRPDEAQRLGALWLQSLIDIQSDTHDSSEFLEHVKIDLFPEAVYVFTPKSKILAL
                     PRGATPIDFAYAIHSDVGDHCVAAKVNGEPVALRTELKSGDVVEVSTSPNARPNPSWL
                     NSVRTGRARSKIRHFLKNLEHEESILLGEKLLTQALRNEGMSVPADDEEHAALWQSLT
                     RWSGSRNRAELMVDIGLGRKIASIVAKRLAQLLGERGQRPGAIALTMGRFANNEAAVG
                     QNVVEIDGSEGASVQLATCCRPIPGDEVAGYFGGEGLTVHTKDCSIGRRLRERDAERW
                     MEVDWAEQLTRQFETAVSVLVRNTKGTLAQVAQAISAAEADITHIDMGDERASETTEL
                     RLLVSVRDRLHLADVLRTLKRASSVLRAARVKP"
BASE COUNT          562 a         1416 c         1483 g          659 t
ORIGIN      
        1 ggcgtgggcc tgttcaaggg caacatcgcg gcgcaggtcg gcgcgctcta tcgtcccggc
       61 gacaagcgca acgccagtgc gttccagatc ttcctgctcg cggtgcaggt cgccgtcatc
      121 gtgtcgcccc tggtctgcgg cacgctgggc gagaaggtgg cctggcactg gggcttcgac
      181 gccgccggcg tgggcatgct gatcggcctg atcacctacc tgtcgggccg cggctggttg
      241 ccgccggagc ccgagcgcaa gagcgcgtcg gcgacgatcg aacagccgaa gatcacgcac
      301 cgcgaatggg gccgcgtggc gatcctcgtg ggcatcatcc ccatcctgat gctgaccatc
      361 ctgggcaacc agcagatcag caccggcttt ccgctgtggt cggacaagca catggatctg
      421 gtgatgttcg gctggcgcgt gcccaccacg tggctgcagg tcgtcgacgc cgccgtcagc
      481 ttcgtcacga tgctgctgtc gatcgcgttc tggaagtggt ggagcctgcg ccgcaccgag
      541 cccgacgagc tcgtgaagat gggcatcggc acgctgatcg ccgccgccgg gcccggcgcg
      601 atgatgatcg ccggcggcat cgtcgatgcg acgcatgccc gcgtgggctt cggctggacg
      661 ctggcctaca ccatctgcaa cgacctgggc ttcgccaaca tcctgcccgt gggcctggcg
      721 ctgtattcgc gcgtcgcgcc caagcagatc gtcggtctcg tcgtcggcat ctactacctg
      781 cacctgtcca tcggcaacac gtatgtcggc tggctggccg gcctgatgga gaagatgccc
      841 agcgcgcagt tctggggcat gcacgcggcg ctcgtggcgc tgggcggcgg cctgctgctg
      901 gtcgttcgga tgctgttctg gcgctggctg tcgccggccg cgatcctgcc cgcgggccag
      961 gaagagccgg acgccgcgct cgcgcccgcg cactgacgcg cgtcatgcac gagcgcgcgt
     1021 gagggcgcgc gggcgtgtcg cttgccgccc cggggacgtt tcaccatgtc ccgaaggtgc
     1081 cgccatgcag accccgccgc ccctgatgca cgccgtcctc atccgccgct tcggcgggcc
     1141 cgaggtggtc gagctggccg acgtcccggt gcccgtgccg agcgacggcg agctgctgct
     1201 caaggtggaa gccgcgagcc tgaacccggt cgactggaag atccgcagcg gcaagtaccc
     1261 ggtggtcaag gacgacaagc tgcccttcgt gctgggccgc gacttcagcg cgacggtgct
     1321 gcagtccgat gacgccgcac tgccggccgg cacgctggtg cacggcatgg ccgacatggc
     1381 ccacggggcg ctcgcgcaat atacggtcgc acgtccggga cagctcgccg ccgtgcccgg
     1441 cacgctggac cgcatggccg cggcggcggt gccgctggcc gcgctgaccg cctggcaggg
     1501 cctgttcgac cagggcggac tgcgcgccgg ccaacgggtg ctgatccacg ccggcgcggg
     1561 cggcgtcggc cacttcgccg tgcagttcgc caaggcgcgc ggcgcgcacg tgatcacgac
     1621 ggtgtccacc gacgacgtcg agctcgcccg cagcctgggc gccgacgagg tcatcgacca
     1681 cctggccgag cgcttcgaga ccaagacccg cgacatcgac ctcgtgttcg acctcgtcgg
     1741 cggcgcggtg caggagcact cgtgggcggt gctcaagcgt ggcggcacgc tggtgtcgac
     1801 cgtcggccag ccgccgcagg acaaggccga ggccatcggc gtgcgcgcgc tgggttacca
     1861 ggccaggccg gacgcggacc agctgcgcga gatcgacgcg ctgatccagt cgggcaaggt
     1921 gcgtcccatc gtgtcgcgca agtacgccgt gggcgagttc aaggaggcgc tgcgcaccct
     1981 ggaggacggc cacgtcacgg gcaagctggt cgtggacctg gcgggcgcgg tcgcggacgt
     2041 ccccgacccg atctaccggc ccggcgccta gcctggggcc tcgcaggcag ccggcgcggg
     2101 cacgcattta gtgtgaacag gccctagcgc ggcggaccgc gccaagtcac ggcttgacgc
     2161 gggcggcgcg cagcaccgac gacgcgcgct tgagcgtgcg cagcacgtcc gccaggtgca
     2221 ggcggtcgcg cacgctcacg agcaggcgca gctcggtcgt ctcggaggcg cgctcgtcgc
     2281 ccatgtcgat gtgcgtgatg tcggcctcgg cggcgctgat cgcctgcgcg acctgcgcca
     2341 gcgtgccctt ggtgttgcgc acgaggacgc tgacggccgt ctcgaactgg cgcgtcagct
     2401 gctctgccca gtcgacctcc atccagcgct cggcgtcgcg ctcgcgcagg cgccggccga
     2461 tggagcagtc cttggtgtgc accgtcaggc cctcgccgcc gaagtagccc gcgacctcgt
     2521 cgccggggat cggacggcag caggtggcca gctgcaccga tgcgccctcg ctgccgtcga
     2581 tctcgacgac gttctggccg acggccgcct cattgttggc gaagcggccc atggtcagcg
     2641 cgatcgcgcc cgggcgctgg ccgcgctcgc ccagcagctg cgcgaggcgc ttggcgacga
     2701 tggaggcgat cttgcggccc aggccgatgt cgaccatcag ctcggcgcgg ttgcggctgc
     2761 cgctccagcg cgtgagcgac tgccacagcg cggcatgctc ctcgtcgtcg gccggcacgg
     2821 acatgccttc gttgcgcagc gcctgggtca gcagcttctc gccgagcagg atcgattcct
     2881 cgtgctcgag gttcttgagg aaatggcgga tcttggagcg tgcgcgcccg gtgcgcaccg
     2941 agttcagcca cgacgggttc ggccgcgcgt tgggcgaggt gctcacctcg acgacgtcgc
     3001 cgctcttcaa ctccgtgcgc agggcgacgg gctcgccgtt gaccttggcc gcgacgcagt
     3061 ggtcgccgac gtccgagtgg atcgcgtagg cgaagtcgat cggcgtcgcg ccgcgcggca
     3121 gcgcgaggat cttgctcttg ggcgtgaaga cgtagacggc ctcggggaac aggtcgatct
     3181 tgacgtgctc gaggaactcg ctggagtcgt gcgtgtcgct ctggatgtcg atcagcgact
     3241 gcagccacag cgcgcccagg cgctgcgcct cgtcggggcg gccgccctgc gggccggcct
     3301 tgtacatcca gtgcgccgcg atgccggcct cggccaccgc gtgcatctcc tcggtgcgca
     3361 cctggaactc gaccgccgtg cccagcgggt tcacgagcgt ggtgtgcagc gactggtagc
     3421 cgttggcctt ggggatcgcg atgtagtcct tgaagcggcc gggcaccggc ttgtacatct
     3481 ggtgcagcgt gccgagcgac aggtagcact cgggcagcga gtgcacgacg atgcggaagc
     3541 cgaagatgtc gttgacctgc gcgaagccgg cgtgcttctc gcgcatcttg cggtagatcg
     3601 agaacaccgt cttctcgcgg ctgaacaccg tgaccttgct gcgcgcctcg gcgaacgcct
     3661 tctcgacgtc gcgccgcatg cggtcgacga tgtcgcgccg cgtgccgcgc gcgcgctcca
     3721 gcgccttggc gatcgccgca taccgccagg ggtacatgaa gcggaacgac aggtcctgca
     3781 gctcgcggta catctggttc aggcccaggc ggtgcgcgat gggggcgaag atctcgatgg
     3841 tctcgcgggc gatgcgggcg cgcttggccg gcgccatcgc gtccatcgtg cgcatgttgt
     3901 gcaggcggtc gcacagcttg atgaggatca cgcgcacgtc gcgcgacatc gccagcagca
     3961 tcttgcggaa cgactcggcc tggccgtcct cgcgcgtcga gaactgcagc ttgtccagct
     4021 tggtcaggcc gtcgacgagg tcggcggtgg gcgcgccgaa gcgctcgatc agctcgggct
     4081 tggtgacgcc gcagtcctcg atggtgtcgt gcatcagcgc
//
LOCUS       DATCRO010000942        15555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1000718, whole genome shotgun sequence.
ACCESSION   DATCRO010000942 DATCRO010000000
VERSION     DATCRO010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15555
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000718"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VIN75_25665"
     CDS             complement(<1..1073)
                     /locus_tag="VIN75_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY8977628.1"
                     /translation="MKTRAAVAWKAGAPLSIETVDLDGPKAGEVLVEVKATGICHTDQ
                     FTLSGADPEGLFPAILGHEGAGVVVDVGPGVTTLRRDDHVIPLYTPECRQCKFCLSRK
                     TNLCQQIRSTQGRGLMPDATSRFSLDGKPILHYMGTSTFSNYIVVPEIALAKIRPDAP
                     FDKACYIGCGVTTGVGAVLFTAGVEAGANVVVFGLGGIGLNVIQGARMVGANRIVGID
                     INPAREAMARQFGMTHFINPKDVPNVVDAVVQLTDGGADYSFECIGNTTTMRQALECC
                     HKGWGQSVIIGVAGAGQEIATRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKI
                     DIDSLITHKLKLEDINEGFNLMKR"
     gene            1167..1679
                     /locus_tag="VIN75_25670"
     CDS             1167..1679
                     /locus_tag="VIN75_25670"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977629.1"
                     /translation="MNIRRLSAADAGAFRALRLAALLDKPEAFVTSHVEEKDRPIGYF
                     EERLVERPGHAVFGAFDGDTLVGVAGLARETLIQLAHKGHIWGMYVAEAARGHGVARE
                     LMAAAIAHARATPGIAKVTLSVDSQNVAAIALYESLGFVVFAREADAVRLDGQSRDDL
                     QMHLRLDVAP"
     gene            1772..2314
                     /locus_tag="VIN75_25675"
     CDS             1772..2314
                     /locus_tag="VIN75_25675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977630.1"
                     /translation="MNKTLRTAALAAVTAAFGGWSGAAHAAYTFYDDDGGNGTLSGSY
                     PAFTITGSNDGSGVDDTAFYVQTYASATTISFTWQYESHDTGGSVYDPAGWILDGVET
                     QLSIDGGPGTSSNGSFSVSVGAGQTFGWYVHSFDSMGGAGVLAVNEDLPPAVPEPGDA
                     ALMMAGLAALIAAARRPRNG"
     gene            2405..3313
                     /locus_tag="VIN75_25680"
     CDS             2405..3313
                     /locus_tag="VIN75_25680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8977631.1"
                     /translation="MSNAEPARRDAARETIPSPMVDAELSLGWRTDLIFARFDGHVDD
                     RGNHLCVRTPGNPTFWWGNFLLFQHAPGPGDLERWMALFDEEIASRQPASRHRAFGVD
                     VRERLALPPEFAAAGFTLSEATVLTLARDQMLAPRRPLPEGVEFQVLDLPHDGQAVVD
                     KQVAVDSSRFEPTGYREFAQRQVGRYAAMQAAGLGHWFGLVAQVEGRAVLVASCGLFR
                     ASSRAGDPAARLGRFQYVSTHPAWRRRGLCTALVHAVCRHGFEAMDLDTLVMVADPDD
                     VAIGVYESLGYRRGTSTWQFERAPRA"
     gene            complement(3556..4266)
                     /locus_tag="VIN75_25685"
     CDS             complement(3556..4266)
                     /locus_tag="VIN75_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY8977632.1"
                     /translation="MTTLADFPITRKWPATHPDRLQLYSLPTPNGVKVAIALEETGLP
                     YEPHLVRFDTNDQLSPEFLSLNPNNKIPAIIDPDGPGGAPMPLFESGAILVYIAGKTG
                     QLMPRDDAGRYQVLQWLMFQMGGIGPMFGQVGFFNKFAGKDYEDKRPRDRYVAESRRL
                     LAVLDKHLAGREWIVGDEYSIADIASFPWINNLVTFYGAADLVGFDDFANVKRVLAAF
                     LARPAVQRGLKIPARPAA"
     gene            complement(4334..4993)
                     /locus_tag="VIN75_25690"
     CDS             complement(4334..4993)
                     /locus_tag="VIN75_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY8977633.1"
                     /translation="MNGHALFTFLDLAGTFAFALSGAVAARERGLDWFGVLVLAFTVA
                     CGGGVLRDVCIGAIPPAGLADWRYLAVAMTAGVLTLASRSLVDRLAHPVVLFDTLGLG
                     LFAVTGAQKALLAGSNAEVAMLLGMVTAVGGGLARDVLLNRVPVILQREIYASAALVG
                     AAVATFGEEFGLGSPTLTWCAVAVCFALRALSLRYRWHLPRFQRKPRPEAGDSMNSLG
                     E"
     gene            complement(5017..5175)
                     /locus_tag="VIN75_25695"
     CDS             complement(5017..5175)
                     /locus_tag="VIN75_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977634.1"
                     /translation="MPDPKKTPPPPSDDDLLDEALEETFPASDPIEPPRRDKLPPAHA
                     PGSDVHDQ"
     gene            complement(5196..6089)
                     /locus_tag="VIN75_25700"
     CDS             complement(5196..6089)
                     /locus_tag="VIN75_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8977635.1"
                     /translation="MQVITPRPSDLGDGFLVHRALPSAARRAVGPFVFLDQFGPVQLA
                     PGKGLDVRPHPHIGLATVSYLFEGTILHRDSLGSVVPIQPGEVNWMTAGRGIVHSERS
                     PEDVRAAGARLWGLQFWVALPLEKEQCPPAFAHHGADAIPRVTRPGARVSVVLGEAFG
                     ATSPVEVTSPMFLVDVRLDAGASIDLEVDHPERAAYVLEGEVRGNGAGAPTAREHLIV
                     FDAGEPALLTAGDAPAHVVLLGGAPLDAPRFLWWNFVSSQRDRILEAQAAWSEQRFPA
                     VPGETEFIPLPARPMPPGSAL"
     gene            6231..6950
                     /locus_tag="VIN75_25705"
     CDS             6231..6950
                     /locus_tag="VIN75_25705"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY8977636.1"
                     /translation="MSWKTLRTLRWPPRRLVLWHSIAAIPMATVVVLGAWLSYHYHHL
                     ALDNRERVDRAYAVLDVVDGLYISVQNADVAQRDFIITGDEAHLAVFQAALREQQADA
                     DRLRVLLAASAKQETNLARLEEAVAAKLAELGKTIELRQREGFTAAQLAIRNGDDGQA
                     MTAIRQQVMVLAENERRFLLRRQADTREHERDAVLVGIFIAALSVTTRILITLGIRHV
                     HQKRQAEIVEGAEPAAQGSTG"
     gene            complement(7014..8222)
                     /gene="alaC"
                     /locus_tag="VIN75_25710"
     CDS             complement(7014..8222)
                     /gene="alaC"
                     /locus_tag="VIN75_25710"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEY8977637.1"
                     /translation="MSSSSGKRRFARIDRLPPYVFNITAELKLAARQRGEDIIDLSMG
                     NPDGATPPHIVAKLTEVAQRPDTHGYSASKGIPRLRRAISHWYRDRYQVDIDPDKEAI
                     VTIGSKEGLAHLMLATVDRGDTVLVPDPSYPIHIYGAVIAGADIRSVPMAPGIDFFAE
                     LERAIRGSYPKPKMMILGFPSNPTAQCVELAFFERVIALARKHDIFVVHDLAYADIVY
                     DGWKAPSIMQVPGAKDIAVEFFTLSKSYNMAGWRVGFMVGNPDLVAALARIKSYHDYG
                     TFTPLQVAAIAALEGDQACVEEIRAKYQSRRDVLHKGLTEIGWEVDLPKASMYLWARI
                     PPQYRELGSLEFSKRLLEKAKVCVSPGIGFGDQGDDFVRFALIENESRIRQAVRGIRA
                     MFKADAAIAA"
     gene            complement(8567..10162)
                     /locus_tag="VIN75_25715"
     CDS             complement(8567..10162)
                     /locus_tag="VIN75_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8977638.1"
                     /translation="MTAAAALPADAAPPRPAAGATAAHAPLPPHLLPIFTIAVALSTF
                     MEVLDTSIANVAVPTIAGNLAVSPNMGTWVISSYGLAAAIVVPLTGWIARRFGEVRTF
                     CVSVLLFTIASALCGLATSLPMLVAFRFLQGLVSGPMVPLSQTLLLQNYPPARRGMAM
                     ALWSMTVVVAPIFGPLLGGWITDNATWPWIFYINAPIGVFAAFTAWTLLRQRETPTAT
                     QPIDVVGLVAMVVGVGALQLMLDNGNDLDWFSSGVITALAVIAVVGITFLVIWELTDE
                     HPIVDLSLFRTRNFRFGVIAMALGYMAFFAMTVLIPLWLQTVMNYTPTWAGIATAPVG
                     IFALIVSPLVGRNSHRFDLRVGASLAFVVFGLTAWWFSSFNLDTSLGHIVWPRLLQGV
                     AVAFFFIPINQILLSGVTPSRMAAASGLSNFFRTLSGSMGTAIVTTLWTHRTEVHEVR
                     LAENLTKNGVASTSYLDTLAQGGVSGNAGYAQVQGLVHAQSAMLATSEVFWGIAILFA
                     LLIGFVWLTKPPFGVAGGAAGGH"
     gene            complement(10269..11447)
                     /locus_tag="VIN75_25720"
     CDS             complement(10269..11447)
                     /locus_tag="VIN75_25720"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8977639.1"
                     /translation="MTDDTTPAAAPETPRTGSRAKALTIAGASFALAAIAWAAWQFGY
                     AQYRESTDDAYVQANLVYVNAQVGGTVTALGADDNQPVKAGQVLVALDPSDAAVALAD
                     AGAQLGLAVRQVRQQQDAVGESQAVVAQRRTDLQRAQEDLARRTQLAGTDSIAAEDLQ
                     HARQAVAAAQDALAVAEKTLARTRTAVAGVSLRQNPSVLRARAAYVQASLATQRNDVV
                     APMDGVVARRSVQVGQHVAAGAALLAVVPLQGAWIDANFKEPQLRHIQVGQPVTVSTD
                     LYGGHVEYHGVVASIAAGSGGAFSLLPPQNATGNWIKVVQRVPVRIALDAKELAAHPL
                     RVGLSTDVTVDTHQRSDEQKPAMGLPGANFRTPVFDAQLKTAEARADALIAQEAGSLQ
                     "
     gene            complement(11481..12947)
                     /locus_tag="VIN75_25725"
     CDS             complement(11481..12947)
                     /locus_tag="VIN75_25725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8977640.1"
                     /translation="MNYQYRDYFDGNSVRRLAPLAAAVLALLLAACAPLPPKDAAPAF
                     ATPRATDLVADAPASAAASPGEPALGDYGDAQLGRLIALALQDSPTMAVAQARVRQAD
                     AAAGQTQAAAGASLALDGSATRERYPEHSLYPPPIAGSWRTSGHVALDFAYDFDFWGR
                     NRNALDAALGRATAARADARAAAATLAAGVARTYFQWQAADQHLKIAQQVGAQRQALV
                     ERQARRVRAGLAPGADLAPLDADAAAPRQSIVQLQTQRAQAERQLQALVGVHALPALQ
                     PAPLPAPAAIETADLRLDLLAHRPEVAAARDRVQAALRDVDAQRAAFYPDVTLSALAG
                     FDALQLSSLLHGANREFAATPAIHLPIFDAGRLRAGLASKRADVALAVAQYDQALQQA
                     VADVNDATVRLQGAQREAAPLAGQRLARERDLASAKAREQAGLIDGQQRLVDELVLTA
                     LQDQEITLRLQTLVARIDLDRALGGALPAPARVAAATN"
     gene            complement(13000..13479)
                     /locus_tag="VIN75_25730"
     CDS             complement(13000..13479)
                     /locus_tag="VIN75_25730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8977641.1"
                     /translation="MAAAERAHQRATGGGLGDVAIYILMRTCNIMMGERASRLLEPHG
                     LTAMGYITMMSLYSRPENLANPSELSEVTGETRGNMTRICDELVDKGWMRRVPNAEDR
                     RRVDLSLTDAGMALLNSVVPEMRAQADAFYDRTFTKAEKASLQQLLTKFSAALAAEL"
     gene            complement(13573..13956)
                     /locus_tag="VIN75_25735"
     CDS             complement(13573..13956)
                     /locus_tag="VIN75_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977642.1"
                     /translation="MSERGMAVLALALAVAGCSTAGNGRLVKLDDPEARGLLVPGATT
                     QSQVRQAFGQGTVVRFRSGWETWQYQYREGIPKGWDEVPYIGLVTSRLARPTKELVVL
                     FDDHGVVRRWSLQQFDDREIRPGSP"
     gene            complement(13953..14783)
                     /locus_tag="VIN75_25740"
     CDS             complement(13953..14783)
                     /locus_tag="VIN75_25740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977643.1"
                     /translation="MASSTSTCAATLALLASMGSSGADASGSASEPEPQQVQVTAALP
                     GARDEKTLSRLLAARDAFDHHHALAPEATLAFRLYARLDPQDLARLQLWLVDGGRRTP
                     VPLDDHQRFALSPDWTRALADRGAVLRTNLPDGAVAWHVDVRSPGVPDDGRRLGDLRL
                     ECEADTFHGNLQRGLRTPVSAILDASDELCLRDDAEAWFAERPVFRVTLVDGDRRRVL
                     PYRYLHGSNGTMAMAPLFDWPYALRDRTWFLPLDDRSWPDGTLVQLDAMDAPQPEASP
                     "
     gene            complement(14898..>15555)
                     /gene="fusA"
                     /locus_tag="VIN75_25745"
     CDS             complement(14898..>15555)
                     /gene="fusA"
                     /locus_tag="VIN75_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544034.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8977644.1"
                     /translation="VDVSVGRPQVAYRETPTIESKVTHVHKKQSGGPGQFAGVTLRLR
                     PLARGDGFRFVNESVGGAIPREFVPAIEAGIRRAAQVGVVAGFPTVDFEATVLDGAAH
                     ERDSSSLAFELAAAAAFREAAAKAKPLLLEPVMAVEVITPVDHLGDVIGDLHRRRGDV
                     LGQTQRGGAAVVTANVPLKEMFGYIGHLRAMTSGRAQYSMQTSHYDAAPARELAALAA
                     "
BASE COUNT         2202 a         5456 c         5678 g         2219 t
ORIGIN      
        1 cgcttcatca ggttgaagcc ctcgttgatg tcttcgagct tgagcttgtg ggtgatgagg
       61 ctgtcgatgt cgatcttgcc ttccatgtac cagtccacga tcttgggcac gtcggtgcgc
      121 ccgcgcgcgc caccgaaggc cgaacccttc cagacgcggc ccgtcaccag ctggaacggc
      181 cgcgtcgcga tctcctggcc cgcgccggcc acgccgatga tcacgctctg tccccagccc
      241 ttgtggcagc actccagcgc ctggcgcatc gtggtggtgt tgccgatgca ctcgaagctg
      301 tagtcggcgc cgccgtcggt cagctgcacg acggcgtcga cgacgttcgg cacatccttc
      361 gggttgatga agtgggtcat gccgaactgg cgcgccatcg cctcgcgcgc cgggttgatg
      421 tcgatgccca cgatccggtt ggcccccacc atcctcgcgc cctggatcac gttcagcccg
      481 atgccgccga ggccgaacac gacgacgttg gcgccggcct ccacgccggc cgtgaacagc
      541 acggcgccga cgccggtggt cacgccgcag ccgatgtagc aggccttgtc gaacggcgcg
      601 tcgggccgga tcttcgccag ggcgatctcg ggcaccacga tgtagttgct gaacgtcgag
      661 gtgcccatgt agtgcaggat gggcttgccg tccagcgaaa agcggctggt cgcgtcgggc
      721 atcaggccgc gcccctgcgt cgagcggatc tgctggcaca ggttggtctt gcgcgacagg
      781 cagaacttgc actgccggca ttcgggcgtg tacagcggga tcacgtggtc gtcgcgccgc
      841 agcgtcgtca cgccgggacc gacgtccacc accacgcccg cgccctcgtg gccgaggatg
      901 gccgggaaca ggccttcggg gtcggcgccc gacagcgtga actggtcggt gtggcagatg
      961 ccggtggcct tgacctcgac cagcacctcg ccggccttcg ggccatccag gtcgacggtc
     1021 tcgatgctca gcggggcgcc cgccttccag gcgacggctg cgcgggtctt catgggcttt
     1081 tctccaggag gtctgcgacg cggggacggc gccggtagac tcgtccgcga cgtcccaccg
     1141 accgagcgca aagggtaatc gaagtcatga acatccgccg gctgtccgcc gccgacgccg
     1201 gcgccttccg ggccttgcgc ctggcggcgc tgctcgacaa gcccgaggcc ttcgtcacca
     1261 gccacgtgga ggagaaggac cggcccatcg ggtacttcga ggagcggctg gtcgagcggc
     1321 ccggccacgc ggtgttcggc gcgttcgacg gcgataccct cgtgggcgtc gccggcctcg
     1381 cgcgggagac gctgatccag ctcgcgcaca agggccacat ctggggcatg tacgtggccg
     1441 aggccgcgcg cgggcacggc gtcgcgcgcg agctgatggc ggcggcgatc gcgcacgccc
     1501 gcgcgacgcc gggcatcgcc aaggtgacgc tgagcgtcga ttcgcagaac gtggccgcca
     1561 tcgcgctgta cgaatcgctg ggcttcgtcg tgttcgcccg cgaagccgac gcggtgcggc
     1621 tggacggcca gtcgcgcgac gacctgcaga tgcacttgcg cctggacgtg gccccctagc
     1681 ttgccggccg ggcgcggttg acgtcgacgg ggcgggctcc gacgatcccc gggtcgcgcc
     1741 agggacggcg cgtctcttgc caggaggacg catgaacaag acgcttcgca cggccgcgct
     1801 cgccgccgtc accgccgcat tcggcggctg gtcgggcgcc gcccacgcgg cctacacgtt
     1861 ctacgacgac gacggcggca acggcacgct gtccggcagc tatccggcgt tcacgatcac
     1921 cggctcgaac gatggctcgg gcgtcgacga caccgcgttc tacgtgcaga cctacgcgtc
     1981 cgccaccacg atctcgttca cctggcagta cgagtcccac gacaccggcg gcagcgtcta
     2041 cgacccggcc ggctggatcc tggacggcgt ggagacgcag ctgtcgatcg acggcgggcc
     2101 cggcaccagc agcaacggct cgttcagcgt gagcgtcggc gcggggcaga ccttcggctg
     2161 gtacgtccac tccttcgact cgatgggcgg cgcgggcgtg ctggccgtca acgaggacct
     2221 gccgccggcc gtgcccgagc cgggcgacgc cgcgctcatg atggccggcc tcgcggcgct
     2281 gatcgccgcg gcgaggcgcc cgcggaacgg ctgaatgccg gacaaggcgg cccgcagcgg
     2341 ccgcttcctg cgacaggccg aggcgttcgc ggaatcgctg cgcgagcgtg atccaatcgc
     2401 cggcatgtcc aacgccgaac cggcgcgccg cgatgcggca cgcgagacga tcccgtcgcc
     2461 aatggtcgat gccgagctgt ccctgggctg gcgcaccgac ctgatcttcg cccgcttcga
     2521 cggccacgtc gacgatcgcg gcaaccacct gtgcgtgcgc acgcctggca acccgacctt
     2581 ctggtggggc aacttcctgc tgttccagca tgcgccgggg ccgggcgacc tcgagcgctg
     2641 gatggcgctg ttcgacgagg agatcgcgtc gcgccagccg gcgtcgcgcc atcgcgcgtt
     2701 cggcgtcgac gtgcgcgagc gcctcgcgtt gccacccgag ttcgccgccg ccggcttcac
     2761 gctcagcgag gcgacggtgc tgacgctggc gcgcgaccag atgctggcgc cgcgccggcc
     2821 gctgcccgag ggcgtcgagt tccaggtgct ggatctcccg cacgacgggc aggccgtcgt
     2881 cgacaagcag gtggcggtcg actcgagccg cttcgagccg acaggctacc gcgagttcgc
     2941 gcagcgccag gtcggccgct atgccgcgat gcaggccgcg ggcctcggcc actggttcgg
     3001 cctggtggcc caggtcgaag gccgcgcggt gctggtggct tcgtgcggcc tgttccgcgc
     3061 gtcctcgcgc gccggcgatc cggccgcccg gctgggccgc ttccagtacg tgtccacgca
     3121 tccggcctgg cgccgccgcg gcctgtgcac ggcgctcgtc cacgccgtct gccgccatgg
     3181 cttcgaggcg atggacctgg acacgctggt gatggtggcc gaccccgacg acgtggccat
     3241 cggcgtctac gagtcgctgg gctaccggcg tggcacgagc acctggcagt tcgagcgggc
     3301 gcctcgtgcg tagcaatccg tggcgcgccg atcagcgagg ggtctggcct ctcctcgaat
     3361 cgcgctgaca agtcctcggt agcggctgcg cgtgtcgcga ttcgaggaca ggcctgacct
     3421 cgatcagccg ctgacgggtc gaggtcaggc ctgtccttcg tccgcgatgg gccgaggccg
     3481 ctgcgctcgg ccagacgccg cggacgaagg agaggccaga cccctctggc gtcgtcgcgc
     3541 cgctgtccat aagtcctagg ccgccggcct ggccgggatc ttcagcccgc gctgcaccgc
     3601 cggcctcgcc aggaacgcgg ccagcacgcg cttgacgttg gcgaaatcgt cgaagccgac
     3661 caggtcggcg gcgccgtaga aggtcaccag gttgttgatc cacgggaagc tggcgatgtc
     3721 ggcgatggag tactcgtcgc cgacgatcca ctcgcggccg gcgaggtgct tgtccagcac
     3781 ggccagcagg cggcgcgact cggcgacgta gcggtcgcgc gggcgcttgt cctcgtagtc
     3841 cttgccggcg aacttgttga agaagccgac ctggccgaac atcgggccga tgccgcccat
     3901 ctggaacatc agccactgca gcacctggta gcggccggcg tcgtccctgg gcatcagctg
     3961 ccccgtcttg cctgcgatgt agaccaggat cgcgcccgac tcgaacagcg gcatgggcgc
     4021 gccgcccggc ccgtccgggt cgatgatggc cgggatcttg ttgttcgggt tcagcgacag
     4081 gaactcgggc gacagctggt cgttggtgtc gaagcgcacc aggtgcggct cgtacggcag
     4141 ccccgtctcc tccagcgcga tcgccacctt gacgccgttg ggcgtgggaa gcgagtacag
     4201 ctgcaggcgg tcggggtggg tcgcgggcca cttccgggtg atcgggaaat cggcgagggt
     4261 cgtcatgaag gggcttccgg ctcggtagga ggcgcgattc tgttccgcgc acgatgccct
     4321 tgccggcgcg ggcctactcg cccagcgagt tcatcgaatc gccggcctcc ggccgcggct
     4381 tgcgctggaa acgcggcagg tgccagcggt agcgcagcga cagcgcacgc agcgcgaagc
     4441 acacggccac cgcgcaccag gtgagcgtgg gcgaccccag gccgaactcc tcgccgaagg
     4501 tcgcgaccgc cgcgccgacc agcgccgccg aggcgtagat ctcgcgttgc aggatgaccg
     4561 gcacgcgatt gagcagcacg tcgcgcgcga gcccgccgcc caccgcggtg accatgccca
     4621 gcagcatcgc gacttcggcg ttcgagccgg ccagcagcgc cttctgcgcg ccggtgaccg
     4681 cgaacaggcc caggcccagc gtgtcgaaca gcaccaccgg gtgggcgagc cggtcgacga
     4741 gcgaacgcga cgcgagcgtc agcacgccgg cggtcatcgc gaccgccagg tagcgccagt
     4801 cggccaggcc cgcgggcggg atcgcgccga tgcacacgtc gcgcagcacg ccgccgccgc
     4861 aggccacggt gaaggcgagc accagcacgc cgaaccagtc caggccgcgc tcgcgcgccg
     4921 ccaccgcgcc gctgagtgcg aacgcgaacg tgcccgcgag gtccaggaag gtgaacagcg
     4981 cgtggccgtt cagcaccagc gtgagcgcgt gcgcgttcac tggtcgtgca cgtcgctgcc
     5041 gggcgcgtgg gcgggcggca gcttgtcgcg gcgcggcggc tcgatcgggt cgctggcggg
     5101 gaaggtctct tccagcgcct cgtcgagcag gtcgtcgtcg gagggcggtg gcggcgtctt
     5161 cttcgggtcg ggcatggcga acctcgcgtg atgcgtcaca gggccgaacc cggcggcatc
     5221 gggcgtgccg gcagcgggat gaactcggtc tcgccgggca ctgccgggaa gcgctgctcc
     5281 gaccaggcgg cctgcgcctc caggatgcgg tcgcgctggc tggacacgaa gttccaccac
     5341 aggaagcgcg gggcgtccag cggcgcgccg cccagcagca ccacgtgcgc cggcgcgtcg
     5401 ccggccgtga gaagcgcggg ctcgccggcg tcgaacacga tcaggtgttc cctggccgtg
     5461 ggcgcgccgg cgccgttgcc gcgcacttcg ccttcgagca cgtaggccgc gcgctcgggg
     5521 tggtcgacct cgagatcgat cgacgcgccg gcatccagcc gcacgtcgac caggaacatc
     5581 ggcgacgtca cctccaccgg cgacgtcgcg ccgaaggcct cgcccagcac gacgctcacg
     5641 cgcgcgccgg ggcgcgtcac gcgcgggatc gcgtcggcgc catggtgcgc gaacgccggc
     5701 gggcactgct ccttctcgag cggcagcgcc acccagaact gcaggcccca caggcgcgcg
     5761 cccgcggcgc gcacgtcctc gggcgagcgc tccgagtgca cgatgccgcg gccggccgtc
     5821 atccagttca cctcgccggg ctggatcggc acgacgctgc cgaggctgtc gcggtgcagg
     5881 atcgtgcctt cgaacaggta gctgacggtg gccaggccga tgtgcgggtg cggccgcacg
     5941 tccaggccct tgcccggagc gagctgcacg gggccgaact ggtccaggaa cacgaagggc
     6001 ccgacggcgc gtcgcgcggc gctcgggagc gcgcggtgga cgaggaagcc gtcgccgaga
     6061 tcggaggggc ggggcgtgat cacttgcatg gcgggctttc gggaagggga ttcggggcgg
     6121 cgccacccat ggtaacggcc cgccgatcga ggacgcgcgc gcaagggaac gcgacaggac
     6181 cgaggcttgc cctgcactgc ctggaacgac ctcgtccgcc ggaaacgccc atgtcgtgga
     6241 agaccctcag gacgctgcgc tggccgccgc gtcgcctcgt gctgtggcac tcgatcgccg
     6301 ccatcccgat ggccacggtg gtggtgctgg gcgcgtggct gtcgtaccac taccaccacc
     6361 tggccctgga caaccgcgaa cgcgtcgacc gcgcctacgc ggtgctggac gtggtggacg
     6421 gcttgtacat ctcggtgcag aacgcggacg tcgcgcagcg cgacttcatc atcaccggcg
     6481 atgaagcgca cctggccgtg ttccaggccg cgctgcgcga gcagcaggcc gacgccgacc
     6541 gcctgcgcgt gctgctggcg gccagcgcga agcaggagac caacctggcc cggctcgagg
     6601 aggcggtcgc cgccaagctg gccgagctgg gcaagacgat cgaactgcgc cagcgcgagg
     6661 gcttcacggc ggcgcagctt gcgatccgca acggcgacga cggccaagcg atgaccgcga
     6721 tccgccagca ggtgatggtg ctggccgaga acgagcgccg cttcctgctg cgccgccagg
     6781 ccgacacgcg cgagcacgag cgcgatgccg tgctcgtggg gatcttcatc gcggcgctgt
     6841 cggtgacgac gcgcatcctc atcacgctgg gcatccgcca tgtgcaccag aagcgccagg
     6901 cggagatcgt ggaaggcgcg gaaccggccg cgcagggatc gactggctaa cgccgcgacc
     6961 tgcgcatgaa catcgccctg ggcgcggcgc ccgcacccct cgcgtccccg cgctcaggcg
     7021 gcgatcgccg cgtccgcctt gaacatcgcc cggatgccgc gcaccgcctg gcggatgcgc
     7081 gactcgttct cgatcagcgc gaagcggacg aagtcgtcgc cctggtcgcc gaaaccgatg
     7141 cccggcgaca cgcagacctt ggccttttcc agcagccgct tggagaactc gagcgatccg
     7201 agctcgcggt attgcggcgg gatgcgggcc cacaggtaca tcgaggcctt gggcaggtcg
     7261 acctcccagc cgatctcggt caggcccttg tgcagcacgt cgcggcgcga ctggtatttc
     7321 gcgcggatct cctccacgca ggcctggtcg ccctccagcg ccgcgatggc ggccacctgc
     7381 agcggcgtga acgtgccgta gtcgtggtag ctcttgatgc gcgccagcgc cgccaccagg
     7441 tcggggttgc ccaccatgaa gcccacgcgc cagccggcca tgttgtagct cttgctgagc
     7501 gtgaagaact ccaccgcgat gtccttggcg ccgggcacct gcatgatgga cggcgccttc
     7561 cagccgtcgt agacgatgtc ggcgtaggcc aggtcgtgca cgacgaagat gtcgtgcttg
     7621 cgcgccagcg cgatcacgcg ctcgaagaag gcgagctcca cgcactgcgc ggtcgggttc
     7681 gacgggaagc ccaggatcat catcttgggc ttggggtagc tgccgcggat ggcgcgctcg
     7741 agctcggcga agaagtcgat gccgggggcc atcggcaccg agcggatgtc ggcgcccgcg
     7801 atcaccgcgc cgtagatgtg gatcgggtag ctcgggtcgg gcaccagcac cgtgtcgccg
     7861 cggtcgacgg tggcgagcat caggtgggcg aggccttcct tggagccgat cgtgacgatg
     7921 gcttccttgt cgggatcgat gtcgacctgg taccggtcgc ggtaccagtg cgagatcgcg
     7981 cgccgcagcc gcgggatgcc tttcgaggcg gagtagccgt gcgtgtccgg ccgttgcgcg
     8041 acctcggtga gcttggcgac gatgtgcggc ggcgtggcgc cgtcgggatt gcccatgctc
     8101 aggtcgatga tgtcctcgcc acgctggcgc gcggcgagct tcagttcggc cgtgatgttg
     8161 aagacgtacg gggggaggcg atcgatgcgc gcgaaacggc gcttgcccga ggaagaggac
     8221 atggatgaac tttcacgtga gcgcccggaa ccgtccgagc gacgtcgacc cgtcgatggg
     8281 gatgggcctg aggccatcat agcgttgggc gcatcgtccg ggcctccatg ttgcttcgtg
     8341 ccgactgcgc cgtagcgaca cgctgcaggg cgggttagcc cgcaggcgta acccgccatg
     8401 gcggcgttcc ggcacgtccc cggatggcgg gttacgcctg cggctaaccc gccctacgaa
     8461 gacaggccgg tcgcgcaagc ctttcgtcca tcacgagaag cggtgccgac accgcggcct
     8521 gcgagtcgcg tcaagcccga cggcgcgcgt cggtgccggc aatccttcag tgaccacccg
     8581 ccgcgccgcc agccaccccg aacggcggct tcgtcagcca cacgaacccg atcagcagcg
     8641 caaagagaat cgcgatcccc cagaacacct cgctcgtcgc cagcatcgcg ctctgcgcat
     8701 ggaccagccc ctgcacctgc gcatagcccg cattgcccga caccccgccc tgcgccagcg
     8761 tgtccaggta ggacgtcgac gccacgccgt tcttggtcag gttctccgcc agccgcacct
     8821 cgtgcacctc ggtgcgatgc gtccacagcg tggtcacgat ggccgtgccc atgctgcccg
     8881 agagcgtgcg gaagaagttc gacaggcccg acgccgccgc catgcgcgaa ggcgtcacgc
     8941 ccgacagcag gatctggttg atcgggatga agaagaacgc caccgccacg ccctgcagca
     9001 gccgcggcca gacgatgtgg cccagcgagg tgtccaggtt gaagctggag aaccaccacg
     9061 ccgtcaggcc gaagacgacg aacgcgagcg acgcgcccac gcgcaggtcg aagcggtggc
     9121 tgttcctgcc caccagcggc gagacgatca gcgcgaagat gcccaccggc gccgtggcga
     9181 tgccggccca tgtcggcgtg tagttcatca ccgtctgcag ccacagcggg atcagcaccg
     9241 tcatcgcgaa gaaggccatg tagccgagcg ccatcgcgat cacgccgaag cggaagttgc
     9301 gcgtccggaa cagcgacagg tcgacgatcg gatgctcgtc cgtcagctcc cagatcacca
     9361 ggaacgtgat gccgacgacg gcgatcaccg cgagcgccgt gatgacgccg gacgagaacc
     9421 agtcgaggtc gttgccgttg tccagcatca gctgcagcgc gcccacgccg acgaccatcg
     9481 cgacgaggcc gacaacgtcg atcggctgcg tggccgtggg ggtttcgcgc tgccgcagca
     9541 gcgtccacgc ggtgaaggcc gcgaacacgc cgatcggcgc attgatgtag aagatccacg
     9601 gccatgtcgc gttgtcggtg atccagccgc ccagcagcgg gccgaagatc ggcgcgacga
     9661 ccaccgtcat cgaccacagc gccatcgcca tcccgcgcct ggccggcggg tagttctgca
     9721 gcagcagcgt ctgcgacagc ggcaccatcg ggcccgacac caggccttgc aggaagcgga
     9781 aagccaccag catcggcagg ctcgtggcca ggccgcacag cgccgacgcg atcgtgaaca
     9841 gcagcaccga gacgcagaac gtcctgacct cgccgaagcg gcgcgcgatc cagccggtca
     9901 gcggaacgac gatggccgcg gccagcccgt aggacgagat cacccacgtc cccatgttcg
     9961 ggctcaccgc gaggttgccg gcgatggtgg gcacggcgac gttggcgatc gaggtgtcca
    10021 gcacctccat gaacgtcgac agcgccaccg cgatggtgaa gatgggcagc aggtgcggcg
    10081 gcagcggcgc atgcgcggcg gtggccccgg ccgcgggccg cggcggcgcc gcgtcggccg
    10141 gcagggctgc ggcggcggtc attggagccc ctcccgcgcc gggtggggcg ctccgtcccc
    10201 cgagcgcact gcgggccggc ttggggcggc ccggcgcccg gcccgcaaac gggctggcgc
    10261 gatcctgatc attgcaggct cccggcttcc tgcgcgatca gcgcgtcggc gcgcgcctcg
    10321 gcggtcttga gctgcgcgtc gaagacgggc gtcctgaagt tggcgccggg caggcccatc
    10381 gcgggcttct gctcgtccga gcgctggtgc gtgtcgacgg tgacgtcggt ggacagtccc
    10441 acgcgcagcg gatgcgcggc cagctccttg gcgtcgagcg cgatgcgcac cggcacgcgc
    10501 tgcaccacct tgatccagtt gcccgtggcg ttctgcggcg gcagcagcga gaacgcgccg
    10561 cccgagcctg ccgcgatgct cgcgacgacg ccgtggtatt cgacgtgccc gccatacagg
    10621 tcggtcgaca ccgtcaccgg ctggccgacc tggatgtggc gcagctgcgg ctccttgaag
    10681 ttggcgtcga tccaggcgcc ctgcagcggc acgacggcca gcagcgccgc gcccgcagcc
    10741 acgtgctggc ccacctgcac cgagcggcgt gcgaccacgc cgtccatcgg cgcgaccacg
    10801 tcgttgcgct gcgtggccag cgaggcctgc acgtaggcgg cgcgcgcgcg cagcaccgac
    10861 gggttctgcc tgagcgagac gcccgcgacg gccgtgcgcg tcctggcgag cgtcttctcg
    10921 gcgacggcca gcgcgtcctg cgccgccgcc accgcctgcc tggcgtgctg caggtcctcg
    10981 gccgcgatcg agtcggtgcc tgcgagctgc gtgcggcgtg ccagatcttc ctgcgcacgc
    11041 tgcaggtcgg tgcggcgctg cgcgacgacg gcctggctct cgccgaccgc gtcctgctgc
    11101 tggcgcacct gccgcacggc caggcccagc tgcgcgcccg catcggccag cgcgactgcc
    11161 gcgtccgatg gatcgagcgc gaccagcacc tggccggcct tcaccggctg gttgtcgtcg
    11221 gcgccgagcg cggtgacggt gccgccgacc tgcgcgttga cgtagacgag gttggcctgc
    11281 acgtacgcgt cgtcggtgct ctcgcggtac tgcgcgtagc cgaactgcca ggcggcccag
    11341 gcgatggcgg cgagcgcgaa cgaggcgccg gcgatggtca gcgccttggc gcgcgagccc
    11401 gtcctgggcg tttcgggggc ggcggcgggc gtggtgtcgt cggtcatggt ggctgtcctc
    11461 ttgttcggtg atgcgggtgt tcagttcgtg gcggcggcga cgcgggcggg cgcgggcagc
    11521 gcgccgccga gggcgcgatc gaggtcgatg cgggcgacca gcgtctgcag gcgcagcgtg
    11581 atctcctggt cctgcagcgc ggtcagcacg agctcgtcga cgagccgctg ttggccgtcg
    11641 atcaggccgg cctgctcgcg cgccttcgcg ctcgccaggt cgcgctcgcg ggcgaggcgc
    11701 tggcccgcga gcggcgcggc ctcgcgttgc gcgccctgca gccgcacggt cgcgtcgttg
    11761 acgtcggcga cggcctgctg cagcgcctgg tcgtactgcg cgacggccag cgccacgtcg
    11821 gcgcgcttcg aagcgaggcc cgcgcgcagg cggcccgcgt cgaagatcgg caggtggatc
    11881 gccggcgtgg cggcgaactc gcggttggcg ccgtgcagca gcgagctcag ctgcagcgcg
    11941 tcgaagccgg ccagcgcgct gagggtgacg tcggggtaga acgcggcgcg ttgcgcgtcg
    12001 acgtccctga gcgcggcctg cacgcggtcg cgcgcggcgg ccacctcggg gcggtgcgcg
    12061 agcaggtcca ggcgcaggtc ggccgtctcg atcgcggcgg gcgcgggcag cggcgcgggc
    12121 tgcagcgcgg gcagggcgtg gacgccgacg agcgcctgca actggcgctc ggcttgcgcg
    12181 cgctgggtct ggagctgcac gatgctctgg cgcggcgccg cggcgtcggc atccagcggc
    12241 gcgaggtcgg cgcccggcgc gaggcctgcg cgcacgcggc gcgcctggcg ttcgaccagg
    12301 gcctggcgct gcgccccgac ctgctgcgcg atcttcaggt gctggtcggc ggcctgccac
    12361 tggaagtagg tcctggcgac gccggcggcc agcgtcgcgg ccgcagcccg cgcgtcggcg
    12421 cgcgcggcgg tggcgcggcc gagcgcggcg tcgagcgcgt tgcggttgcg gccccagaag
    12481 tcgaagtcgt aggcgaagtc gagcgcgacg tggccgctgg tgcgccagga gccggcgatc
    12541 ggcggcggat agaggctgtg ctccggatag cgctcgcgcg tggccgagcc gtcgagcgcg
    12601 aggctggcgc cggccgctgc ctgggtctgg ccggcggccg cgtcggcctg gcgcacgcgc
    12661 gcctgcgcga cggccatcgt cggcgagtcc tgcagcgcga gcgcgatgag gcggcccagt
    12721 tgcgcatcgc cgtagtcgcc gagtgccggc tcgcccggcg aggccgcggc ggatgcgggc
    12781 gcgtcggcga cgaggtcggt ggcgcggggc gtggcgaagg cgggcgccgc gtccttgggc
    12841 ggcagcggcg cgcacgcggc gagcagcagc gccagcaccg cggcggcgag cggcgcaagg
    12901 cgccggacgg agtttccgtc gaaatagtca cgatattgat agttcatttt gcaaccatat
    12961 gcgcaaaaaa agggcacccg tcgccggatg ccgcgcttgt cagagttcgg ccgccagggc
    13021 cgcggagaac ttggtcagca gctgttgcag cgacgccttc tcggccttgg tgaacgtgcg
    13081 gtcgtagaac gcgtcggcct gcgcgcgcat ctcggggacg accgagttca gcagcgccat
    13141 gccggcgtcg gtcagcgaca ggtcgacgcg gcggcgatcc tcggcgttgg gcacgcggcg
    13201 catccagccc ttgtcgacca gctcgtcgca gatgcgcgtc atgttgccgc gcgtctcgcc
    13261 cgtgacctcg ctcagctccg acgggttcgc caggttctcc ggccggctgt agagcgacat
    13321 catcgtgatg tagcccatgg cggtcaggcc gtgcggctcc agcaggcggc tcgcgcgctc
    13381 gcccatcatg atgttgcagg tgcgcatgag gatgtagatc gcgacgtccc cgagcccgcc
    13441 gccggtggcg cgctggtgtg cgcgttcggc ggcagccatg gtctgcacgg aacgattgcg
    13501 tgtttgattg ttcatattga gatagtaacg catgcaacta tcttcgccaa gcccccgcag
    13561 cccgcggggc gttcagggcg agccgggccg gatctcgcgg tcatcgaact gctgcagcga
    13621 ccagcgccgc acgacgccgt ggtcgtcgaa cagcaccacc agctccttgg tcggacgcgc
    13681 cagccggctg gtgacgaggc cgatgtaggg cacctcgtcc caacccttgg gaatgccttc
    13741 gcggtactgg tactgccagg tctcccagcc gctgcggaac ctgacgacgg tgccctgtcc
    13801 gaaggcctgg cgcacctgag actgcgtggt cgcgcccggc accagcaagc cgcgcgcctc
    13861 cgggtcgtcc agcttcacca ggcggccgtt gccggccgtg ctgcagccgg cgacggccag
    13921 cgcgagcgcc aggacggcca tgccgcgctc actcacggcg acgcctccgg ctgcggcgcg
    13981 tccatcgcgt ccaattgcac cagcgttcca tcgggccacg agcggtcgtc cagcggcagg
    14041 aaccaggtgc gatcgcgcaa cgcatagggc cagtcgaaca gcggcgccat cgccatcgtg
    14101 ccgttgctgc cgtgcaggta gcgatagggc aggacgcggc ggcggtcgcc gtccacgagc
    14161 gtgacgcgaa agaccggccg ctccgcgaac caggcttcgg cgtcgtccct caggcacagc
    14221 tcgtcgctcg cgtcgaggat ggccgacacc ggcgtgcgca gtccgcgctg caggttgccg
    14281 tggaaggtgt cggcctcgca ttcgagccgc aggtcgccga ggcggcgccc gtcatcgggg
    14341 acgccgggcg atcgcacgtc gacatgccag gcgacggcgc cgtcggggag gttggtgcgc
    14401 aggacggcgc cgcggtcggc gagggcgcgc gtccagtcgg gggacagcgc gaagcgctgg
    14461 tggtcgtcga gcggcacggg cgtgcgccgg ccgccatcga ccagccacaa ctgcaggcgc
    14521 gccaggtcct gcggatcgag gcgcgcgtag aggcggaacg cgagtgtcgc ctcgggcgcg
    14581 agggcatggt ggtggtcgaa ggcgtcccgt gcggcgagca gtcgggacag ggtcttctcg
    14641 tcgcgcgcgc cgggcagggc ggcggtgacc tggacttgct gcggttcggg ttcgctggcg
    14701 gagcctgacg cgtccgctcc ggacgagccc atcgaagcca gcagcgccag cgtcgccgcg
    14761 catgtcgatg tcgagctcgc catggccgga ttgcggcaac cgggagtccc ggcgccggcc
    14821 ggctgactcc tcccggtgcg tcgccgaaaa aaagggcgcg caccctcttc ggatgcgcgc
    14881 ccttcgggcc gcgtcggtca cgcggccagc gccgccagct cgcgcgccgg cgccgcgtcg
    14941 tagtggctcg tctgcatcga gtactgcgcg cgacccgagg tcatggcgcg caggtggccg
    15001 atgtagccga acatctcctt gagcggcacg ttcgcggtga cgacggccgc gccgccgcgc
    15061 tgggtctggc ccagcacgtc gccgcggcgg cggtgcaggt cgccgatgac gtcgcccaga
    15121 tgatcgaccg gcgtgatgac ctcgacggcc atcaccggct cgagcagcaa gggcttggcc
    15181 ttggcggccg cctcgcggaa cgcggcggcc gcggccagct cgaaggccag cgacgacgag
    15241 tcgcgttcgt gcgcggcacc gtccagcacg gtcgcctcga agtcgacggt cgggaaaccg
    15301 gcgaccacgc ccacctgcgc ggcgcggcgg atgccggcct cgatcgccgg gacgaactcg
    15361 cgcgggatcg cgccgccgac gctctcgttg acgaagcgga acccgtcgcc gcgcgccagc
    15421 ggacgcaggc gcagtgtgac cccggcgaac tgcccggggc cgccgctctg cttcttgtgc
    15481 acgtgcgtca ccttgctctc gatcgtcggt gtctcgcggt acgccacctg cggccggccc
    15541 accgacacgt ccacg
//
LOCUS       DATCRO010000943         8462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_528286, whole genome shotgun sequence.
ACCESSION   DATCRO010000943 DATCRO010000000
VERSION     DATCRO010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528286"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VIN75_25750"
     CDS             complement(<1..783)
                     /locus_tag="VIN75_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8977645.1"
                     /translation="MSRWAGLTANELNVLEGVFWLHGPSREALSHRLAFSRTHVNAVI
                     AGMLEASWLDRSGQRESSGGRRAETLCLHRSLGVLLCADLGATSIEVAVLSLDLEVLE
                     RRSETIDVRIGPGLVLSRIRALMREVLAARRLKARDVLAIGMGVPGPVDFATGQLVEP
                     PLMPQWDSFSIRDDLRAEYEAPVWVDNDVNLMALAELWRLKRELPNFLVLKVGTGIGC
                     GVVCHGEVYRGANGSAGDVGHICVDPVGPRCHCGNVGCVETMA"
     gene            complement(1081..2259)
                     /locus_tag="VIN75_25755"
     CDS             complement(1081..2259)
                     /locus_tag="VIN75_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY8977646.1"
                     /translation="MRSIHHFIQFGVAAATALALSACGGGGGSSTSSTQGTLRLGLTD
                     APACGYDHVNVTIQKVRINQSATAADTDSGWTDITLDPALRVDLLTLTNGALTTLGQA
                     PLAAGHYSQIRLVLAANDTASPLANSIVPTGGSEIALTTPSGQQSGLKLNADITIAAD
                     QMADFLLDFDACKSVVRAGNSGKYLLKPVISVIPSFISGVTGYVDPSLGNGGTLVSVQ
                     QAGAVVKATSPDATGKFTLEPVAPGAYDLVVAATGHVTGVLTGVPVADKTITILNTSS
                     SAFDFTATPTGSVGGTITTATTPVDASVDALQTLGNGDVVDIATTNADADTGVYALVL
                     PASAPVVAPYASAASSVSFAPDPAAGVNYTIRATSGGVSKSAQLAVSASAPASASFSF
                     "
     gene            2900..3421
                     /locus_tag="VIN75_25760"
     CDS             2900..3421
                     /locus_tag="VIN75_25760"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEY8977647.1"
                     /translation="MNLKLTGLALGLALSTYGAGALASTTDWGTLGPAGAAAVVLDPP
                     GAIDDIYTFVLAAESDVPAYAIKFLGEDTNIDNATLSLYSGAPGSSSLVGSFLFDEVQ
                     PGTTMTFKDLTSGSYYLEVTGTAQLLGGAYDINASAQSPSPPLTVPEPANMMLLLSGL
                     GMLGVAAARRKRT"
     gene            complement(3642..4070)
                     /locus_tag="VIN75_25765"
     CDS             complement(3642..4070)
                     /locus_tag="VIN75_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977648.1"
                     /translation="MASTAQAAPDQPPGHVTGIGGVFIKGKDPKALAAWYRDVLGMPV
                     QPWGGAALRNDAPGHPPKTAWSVFDASSEHFAPSNASWMINYAVDDMDAMLKRLQDKG
                     VAVLKRTDDDAFGRFAWIMDPEGNKVELWEPKKPPAPAKL"
     gene            complement(4178..5317)
                     /locus_tag="VIN75_25770"
     CDS             complement(4178..5317)
                     /locus_tag="VIN75_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8977649.1"
                     /translation="MGVLSGVRVIEIAGLGPAPFCGMLLADMGADVVLVERGGKNGSG
                     GGDPLSLGRNAIVNRGKRSIALDLKDADAVDAVLRLVEGADALIEGMRPGVMERLGLG
                     PDACLARNPRLVYGRMTGWGQDGPLAQAAGHDINYIALSGALWYSGAPGEPPTAPPTL
                     VGDLGGGALYLAMGVLAAVLSARATGQGQVVDAAIVDGSANLMNLLLSAHAAGQMPFE
                     RGRGLLDGPHWYGTYACADGGFVTVGALEPQFNALLLARLGLADDPDFRNAYDPRRWP
                     RLRERLAALLATQPRAHWVELLEGSDACFAPVLTPREALAHPHLAARGVYAERDGVLQ
                     AAPAPRFSATPSANPGPVPARGEHGEAILREIGLDEAAIARLSRR"
     gene            complement(5320..5577)
                     /locus_tag="VIN75_25775"
     CDS             complement(5320..5577)
                     /locus_tag="VIN75_25775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977650.1"
                     /translation="MNLVMRIGCIAAYALGIAAFTGLIHGPLAAAAAIVTLVLIGLHA
                     LELLFAYRFLHRYRGSMGMSVVLALLFGVLHWAPLARQPKA"
     gene            complement(5582..6652)
                     /gene="mnmH"
                     /locus_tag="VIN75_25780"
     CDS             complement(5582..6652)
                     /gene="mnmH"
                     /locus_tag="VIN75_25780"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8977651.1"
                     /translation="MSSPQDVPPVDTSSLAPLPFDQYALIIDARSPHEFAEDHVPGAV
                     NLPVVDDAEFAEVGIRYKTDQHAAYLIGAQHSFRNLARHTADLISRYKTSDRFLVYCF
                     RGGKRSALWASNLRLIGFTVDVVPGGWKRYRQWVRDSLAVLPRRFEYRVLCGPTNCGK
                     TRLLHELARQGAQVLDLEGLARHRGSLLGDLPGEPQPTQKIFDSLLLDTLRRFDPARP
                     VWVEAESRKVGRLLVPDELTAAMRRARAIHLAVPMPERVRHCREEYAHFAVDPKAMVG
                     KLGALKQVVGGEEMARWHALAEAGAVDELFERLMTAHYDPCYTRSTTREFGPGDGANR
                     LELDSLQPPALADAARRLIALE"
     gene            complement(6667..7455)
                     /gene="phnE"
                     /locus_tag="VIN75_25785"
     CDS             complement(6667..7455)
                     /gene="phnE"
                     /locus_tag="VIN75_25785"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY8977652.1"
                     /translation="MTAPRSHRGAGIACLAAIALAIVASFHYLAVDFGGLFDAQARGR
                     MARFVVEFFPPDVSPAFLRQVGVAALQTLAVSLLGTAIPVVLGGLLALPAAGRLGVAP
                     QWLARLALNALRSVPELVWAALMVLAAGLGPFAGTLALALHTTGVLGRLFAESLENAS
                     PVPEAALRDLGAGRVARFAYGTLPLIAPQCVGYTLYRWEMNIRMATILGFVGGGGLGQ
                     MLYFHLSLFQQGQAATVLLAMTLLVLGDDALSGWARRSLSAQPH"
     gene            complement(7591..8430)
                     /locus_tag="VIN75_25790"
     CDS             complement(7591..8430)
                     /locus_tag="VIN75_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8977653.1"
                     /translation="MSPVAAEHPSLRDPASGRRLAWAVTALVLLWPLGRLADFHPATL
                     LALDNVKVLGTFLAGYVPPSTAPDFLRELLTATLTTLAIATAGIALAFVIAVPLAIAA
                     TRSLSVAAIGPADGRWRGRALRQAVRTVTVLLRSVPEIVWALLFVRASGLGPAAGVLA
                     LAITYGGMLAKVYSEILESADIRPARALLQAGSGRLAALCFGLLPLAADELVSYTVYR
                     WECAVRAAVVMGFVGAGGLGQLMDQAIKGFVPSEASSISLVFLALVLLADGISLVLRK
                     VLA"
BASE COUNT         1282 a         3006 c         3045 g         1129 t
ORIGIN      
        1 ggccatggtc tcgacgcagc cgacgttgcc gcagtggcag cgcggcccca ccgggtcgac
       61 gcagatgtgg ccgacgtcgc cggccgaccc gttggcgccg cggtagacct cgccgtggca
      121 cacgacgccg cagccgatgc ccgtgcccac cttgagcacc aggaagttgg gcagctcgcg
      181 cttgaggcgc cacagctcgg ccagcgccat caggttgacg tcgttgtcga cccagacggg
      241 ggcctcgtat tcggcgcgca ggtcgtcgcg gatcgagaag ctgtcccact gcggcatcag
      301 cggcggctcg accagctggc cggtggcgaa gtccacgggg cccggcacgc ccatgccgat
      361 cgccaggacg tcgcgcgcct tgagcctgcg cgcggccagc acctcgcgca tcagcgcccg
      421 gatgcgcgac agcaccaggc ccgggccgat gcgcacgtcg atagtctcgc tgcggcgctc
      481 cagcacctcg aggtcgagcg agagcacggc gacttcgatg ctcgttgccc cgaggtcggc
      541 gcacagcagc acgcccagcg agcgatgcag gcacagcgtc tccgcgcgcc ggccgccgga
      601 cgattcgcgc tggcccgagc ggtcgagcca gctggcctcg agcatgccgg cgatcaccgc
      661 gttgacgtgc gtgcgggaga acgccagccg atgcgacagc gcctcgcgcg acggaccatg
      721 cagccagaac acgccttcga ggacgttcag ctcgttcgcg gtcaatccgg cccagcggct
      781 catcgcctcg tgtctccgtc ctggcgacct cttcgggtcg atggcggact gtagggcggc
      841 gacttcggcc ctgcaatgtc tgacttcggc cgattcacgg ccaaagcgcg cctccacggg
      901 gacgcacgga tcgcggcagg cgcgaagggt gcgtggcgcc gtggtcgacg cctcgacgcg
      961 cccgtccgac cggatcctgg aacggatgcc gttccgatca gatccggtta cgaatgtgcg
     1021 aattgcacag ccataaaaac aacacgacag gagcccttgc cgggcccctg ccgcgcaatc
     1081 tcagaacgag aagctcgcgc tggccggcgc gctggcggac accgccagct gcgccgactt
     1141 cgacacgcca cccgaggtcg cccggatcgt gtagttgacg ccggccgccg gatccggcgc
     1201 gaaggagacg ctggacgcgg ccgacgcgta cggcgcgaca accggagccg acgcgggcag
     1261 caccagcgcg tagacgccgg tgtccgcgtc ggcgttggtg gtcgcgatgt cgaccacgtc
     1321 gccgttgccc agcgtctgca gcgcgtccac ggaggcatcg accggcgtgg tggccgtggt
     1381 gatcgtcccg cccacggagc ccgtcggggt cgccgtgaag tcgaacgcgg aggacgacgt
     1441 gttgaggatc gtgatcgtct tgtcggccac cggcacgccg gtcagcacgc ccgtcacgtg
     1501 gccggtcgcg gcgacgacga ggtcgtaggc gccgggcgcc acgggctcga gcgtgaactt
     1561 gccggtcgca tccggcgacg tggccttcac gacggcgccc gcctgctgca ccgacaccag
     1621 cgtcccgccg ttgcccaggc tggggtcgac gtagcccgtg acgcccgaga tgaagctcgg
     1681 gatcaccgag atcaccggct tgagcaggta cttgccggag ttgcccgcgc gcacgaccga
     1741 cttgcaggcg tcgaagtcga gcaggaaatc ggccatctgg tcggcggcga tcgtgatgtc
     1801 ggcgttcagc ttcaggccgc tctgctggcc gctgggcgtg gtcagcgcga tctcggaacc
     1861 cccggtgggc acgatggaat tggcaagcgg ggacgccgtg tcgttggccg ccagcacgag
     1921 acggatctgc gagtagtggc ccgcggccag cggtgcctgt ccgagcgtcg tcagcgcgcc
     1981 gttggtcagc gtcagcaggt cgacgcgcag cgcgggatcg agcgtgatgt cggtccagcc
     2041 ggagtcggtg tccgccgcgg tcgcgctctg gttgatgcgc accttctgga tcgtcacgtt
     2101 gacgtggtcg tagccgcacg ccggggcgtc cgtgagcccg aggcgcagcg tgccttgcgt
     2161 gctcgacgtg gacgagccgc cgcccccgcc gcaggcgctg agcgccaggg ccgtggccgc
     2221 ggccacgccg aactggatga agtgatggat ggatctcatg tctgttctcc tggaaagttc
     2281 ccgcgggccc tcgcccgctc ggaacggaac actggcggtt cctggatgaa tgtccccgtg
     2341 tcgatggcgg cgcctcacgc gccgcgcacc ggccagcgta ggcagcgaag ctgtcggaag
     2401 cgactcccct cgtaacgact tgtggacgcc cgctacgcat cgccatcggt gcgccgacgc
     2461 catcgatcgc catggcggat gcggccagga cgcgggcagg cactaacccc atgctgtatg
     2521 gcggttcgca cttgcagcaa gaagcgcatc gctcgcgcgc catgcatgag tgcgaatttc
     2581 caccgccgcc tgcctcgcgc cgatcgcggc gaaccagccg ctgcggcgcg gcgatcgtcc
     2641 gggcctcgga cctccccgaa tcggttgggt gtttaccctt ggaacctcga tcgacgaccc
     2701 accggaatcg atcgtttcgc gggcccggga agcgcacgtc caccgggcgt agaggccttg
     2761 ttgcgccgcc tgcgacgaac tcgcgtggcg acctccgatc tcgcgcacgg gcgctgtaac
     2821 caggtgtacg aattgcccag tctggccagc cgccgatcgg gcggcgagag atcgacaaca
     2881 ctggaatcgg agactgacca tgaacctgaa gctcacaggc ctggccctgg gcctcgcact
     2941 atcgacatat ggcgcggggg ccctcgcgtc caccaccgac tggggcacgc tgggccccgc
     3001 cggcgccgcc gccgtcgtcc tggatccccc gggcgcgatc gacgacatct acacgttcgt
     3061 gctggccgcc gaatcggacg tgcccgccta cgcgatcaag ttcctcggcg aggacaccaa
     3121 catcgacaac gcgacgctgt cgctgtactc cggcgccccg ggctcgtcgt cgctggtggg
     3181 ctcgttcctg ttcgatgaag tgcagcccgg caccacgatg acgttcaagg acctcaccag
     3241 cggctcgtac tacctcgagg tgacgggcac cgcccagctg ctgggcgggg cgtacgacat
     3301 caacgcctcg gcgcagtcgc cgtcgccgcc gctgaccgtg ccggaaccgg ccaacatgat
     3361 gctgctgctg tccggcctgg gcatgctggg cgtcgcggcc gcccgccgca agcgcacctg
     3421 agccaggaag ccgcaggctc ttgcaggccg gccagggacc gaacacggac ctggccggcc
     3481 gtttgtcgtc tagaagccgt gaatgaatcc ggcgcgtccg agcggcgcgc caaaacgcgt
     3541 ctgatcgagg cgcaaagcac agacgtggcc cgggccacgg cgagcatttg caacgacgag
     3601 caggcctgtt ttggcctgcc gagcgggcgt gcgggattcg ttcacagctt cgcgggcgcc
     3661 ggtggtttct tcggctccca caactccacc ttgttgccct ccggatccat gatccaggcg
     3721 aagcggccga acgcgtcgtc gtccgtgcgc ttgagcaccg cgacgccctt gtcctgcagg
     3781 cgcttgagca tcgcgtccat gtcgtcgacg gcgtagttga tcatccagct ggcgttggac
     3841 ggcgcgaagt gctccgacga tgcgtcgaac acgctccatg ccgtcttggg cgggtggccg
     3901 ggcgcgtcgt tgcgcagcgc ggcgccgccc cagggctgga ccggcatgcc caggacgtcg
     3961 cggtaccacg cggcgagcgc cttgggatcc ttgcccttga tgaagacgcc gccgatgccg
     4021 gtgacgtggc cgggcggttg atcgggtgcg gcttgggccg tgcttgccat gatgagtgct
     4081 ccggacagga gggccaggat cgggtgcgcg cgcatgcgtg ctcctcggcg agggattcga
     4141 ggccgccact gtacgcccgc ggcgagcggc cggcgcttca gcggcgtgac aggcgcgcga
     4201 tcgccgcctc gtccaggccg atctcgcgca ggatcgcctc gccgtgctcg ccgcgcgccg
     4261 gcacgggacc ggggttcgcc gacggcgtgg ccgagaaccg cggcgccggt gccgcctgca
     4321 gcacgccgtc gcgctccgcg tacacgccgc gcgcggccag gtgcgggtgc gcgagcgcct
     4381 cgcgcggcgt gagcaccggc gcgaagcacg cgtcgctgcc ttcgagcagc tcgacccagt
     4441 gcgcgcgcgg ctgcgtcgcc agcagcgcgg ccaggcgctc gcgcaggcgg ggccagcggc
     4501 gcgggtcgta ggcgttgcga aagtcgggat cgtcggccag gcccagcctg gcaagcagca
     4561 gcgcgttgaa ctgcggctcg agcgcaccca cggtcacgaa gccgccgtcg gcgcaggcgt
     4621 aggtgccgta ccagtgcggg ccgtcgagca ggccgcggcc gcgctcgaac ggcatctggc
     4681 cggcggcgtg cgccgacagc agcaggttca tcaggttggc gctgccgtcg acgatggcgg
     4741 catcgacgac ctggccctgc ccggtcgcgc gagcggacag caccgcggcc agcacgccca
     4801 tcgccaggta cagcgcgccg ccgcccaggt cgccgaccag cgtgggcggc gccgtcggcg
     4861 gctcgcccgg cgcgccggaa taccagagcg cgccggacag cgcgatgtag ttgatgtcgt
     4921 gccccgcggc ctgcgccagc gggccgtcct ggccccagcc ggtcatgcgg ccgtagacca
     4981 gccgcgggtt gcgcgcgagg caggcgtcgg ggcccaggcc caggcgctcc atcacgccgg
     5041 ggcgcatgcc ctcgatcagc gcgtcggcgc cttccaccag tcgcagcacc gcgtcgacgg
     5101 cgtccgcgtc cttcaggtcc agggcgatcg agcgcttgcc gcggttgacg atggcgttcc
     5161 tgcccagcga cagcggatcg ccgccgcccg agccgttttt cccgccgcgc tcgacgagca
     5221 cgacgtccgc gcccatgtcg gccagcagca tgccgcagaa cggcgccggg ccgaggccgg
     5281 cgatctcgat gacacgaacg ccggacagca cccccatggt caggccttcg gttgacgggc
     5341 cagaggcgcc caatgcagca cgccgaacag cagcgccagc acgacgctca tgcccatcga
     5401 gcccctgtag cgatgcagga agcggtacgc gaacagcagc tcgagcgcgt gcaggccgat
     5461 cagcacgagc gtgacgatgg ccgcagcggc cgcgagcggg ccgtggatca ggccggtgaa
     5521 ggcggcgatg cccagtgcgt aggcggcgat gcagccgatt ctcatgacga ggttcatggg
     5581 gtcactccag ggcgatcagg cggcgcgcgg cgtcggccag cgctggcggt tgcagcgagt
     5641 ccagctcgag ccggttggcg ccgtcgccgg ggccgaactc gcgcgtggtg gaacgcgtgt
     5701 agcaggggtc gtagtgcgcg gtcatcaggc gctcgaacag ttcgtccacc gcgccggcct
     5761 cggccagcgc gtgccagcgc gccatctcct cgccgcccac gacctgcttg agcgcgccga
     5821 gcttgcccac catcgccttg gggtcgacgg cgaaatgcgc gtactcctcg cggcagtggc
     5881 gcacgcgctc gggcatgggg acggccaggt ggatggcacg ggcgcggcgc atcgcggcgg
     5941 tcagttcgtc gggcaccagc aggcggccga ccttgcggct ctcggcctcg acccacacgg
     6001 gccgcgcggg atcgaagcgg cgcagcgtgt ccagcagcag cgagtcgaag atcttctgcg
     6061 tgggctgcgg ctcgcccggc aggtcgccca gcagcgagcc gcggtgcctg gcgaggccct
     6121 cgaggtcgag cacctgcgcg ccctggcgcg cgagctcgtg cagcaggcgc gtcttgccgc
     6181 agttggtcgg accgcacagc acgcggtact cgaagcgccg gggcagcacc gccagcgagt
     6241 cgcgcaccca ctggcgatag cgcttccagc cgcccggcac gacgtcgacg gtgaagccga
     6301 tcagccgcag gttgctggcc cacagcgcgc tgcgcttgcc gccgcggaag cagtagacga
     6361 ggaagcggtc gctcgtcttg tagcgcgaga tgagatcggc cgtgtgccgg gcgaggttgc
     6421 ggaatgaatg ctgcgcgccg atcaggtagg cggcgtgctg gtcggtcttg taccggatgc
     6481 cgacctcggc gaattcggcg tcgtcgacca ccggcaggtt caccgcgccc ggcacgtggt
     6541 cctccgcgaa ttcgtgcggc gagcgcgcat cgatgatcag cgcgtactgg tcgaacggca
     6601 gcggagccag cgacgaggtg tcgaccggcg gaacgtcttg cggggagctc atcgcgccat
     6661 tttcggtcaa tgcggctgcg cgctcagcga acggcgcgcc cagccgctca gcgcgtcgtc
     6721 gcccagcacg agcagcgtca tcgccagcag caccgtcgcc gcctgcccct gctggaacag
     6781 cgacaggtgg aaatacagca tctgcccgag cccgccgcca cccacgaagc ccaggatggt
     6841 ggccatgcgg atgttcatct cccagcggta gagcgtgtag cccacgcact gcggcgcgat
     6901 cagcggcaag gtgccgtagg caaagcgcgc gacgcggccg gcgccgaggt cgcgcagcgc
     6961 ggcttcgggc acgggcgagg cgttctccag gctctcggcg aacagccggc ccagcacgcc
     7021 cgtggtgtgc agcgcgagcg cgagggtgcc ggcgaacgga cccaggcccg cggccagcac
     7081 catcaaggcc gcccacacga gctcgggcac gctgcgcaac gcgttgagcg ccaggcgcgc
     7141 gagccattgc ggcgcgacgc ccaggcggcc cgcggcgggc agcgcgagca ggccgcccag
     7201 caccacgggg atcgccgtgc ccagcagcga gacggccagc gtctgcagcg ccgcgacacc
     7261 cacctggcgc aggaacgcgg gcgagacgtc gggcgggaag aactcgacga cgaagcgcgc
     7321 catgcggccg cgcgcctgcg cgtcgaacag gccgccgaag tcgaccgcga ggtagtggaa
     7381 gctggcgacg atggccagcg cgatcgccgc caggcaggcg atgcccgcgc cgcgatggct
     7441 gcgcggggcg gtcatcggcc actctccgcc ctgcgggctg cggtcgattc gcccgtcggg
     7501 tggccgtgcg ggttcatgag gcaagctccg tcctgcggac tgcggtgtga ccgacgcagc
     7561 aggaaacgcc ttcgggcggc cgggcggcgc tcacgcgagc accttgcgca gcacgaggct
     7621 gatcccgtcc gcaagcagca ccagggcgag gaacaccagc gagatgctgc tcgcctcgga
     7681 cggaacgaag cccttgatcg cctggtccat cagctggccc aggccgccgg cgccgacgaa
     7741 gcccatcacc accgcggcgc gcaccgcgca ttcccagcga tagacggtgt acgagaccag
     7801 ttcgtcggcc gccagcggca gcagcccgaa gcacagcgcg gcgagacggc cgctgccggc
     7861 ctgcagcagc gcccgcgcgg gccggatgtc ggccgactcc aggatctccg aatagacctt
     7921 ggccagcatg ccgccatagg tgatggccag cgcgagcacg cccgcggcgg gtcccagccc
     7981 cgaggcgcgc acgaacagca gcgcccacac gatctcgggc acggagcgca gcagcacggt
     8041 caccgtgcgc accgcctggc gcagcgcgcg cccgcgccag cggccgtcgg ccgggccgat
     8101 cgcggcgacg gacagcgagc gcgtcgcggc gatggccagc ggcaccgcga tcacgaaggc
     8161 cagcgcgatg ccggcggtgg cgatcgccag cgtggtcagc gtggccgtca gcagctcgcg
     8221 caggaagtcg ggcgcggtcg acggcggcac gtagccggcc aggaacgtgc ccagcacctt
     8281 gacgttgtcc agtgccagca gcgtggcggg atggaagtcg gccaggcggc ccagcggcca
     8341 cagcagcacc agcgccgtga ccgcccacgc caggcgacgg cccgacgccg gatcgcgcag
     8401 cgacgggtgc tccgcggcga ccggcgacat caggcaaccc tccgcctgtc gcctgcggcg
     8461 cg
//
LOCUS       DATCRO010000944         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_404475, whole genome shotgun sequence.
ACCESSION   DATCRO010000944 DATCRO010000000
VERSION     DATCRO010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404475"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VIN75_25795"
     CDS             complement(<1..539)
                     /locus_tag="VIN75_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8977654.1"
                     /translation="MLLNRPLRQPGRLLAALLLGGATLAAWAGATPSDARPTLAPTSP
                     LVKPPAAASAAAQEDEHDDSANLSASARRIYDVGRPRLLQVRTLLRDQDSQASVGSGS
                     LVGPGGLILTNYHVVSQVALEPQRYRLNFQTVDGVTGALQLVAFDVVHDLALVRMAAP
                     PADVASRGLFQIRPDTGAAL"
     gene            646..1239
                     /locus_tag="VIN75_25800"
     CDS             646..1239
                     /locus_tag="VIN75_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977655.1"
                     /translation="MHAPRDVASIRLDNALALFDEFVRNTIKHPDAATLRGLERRFAE
                     RLQIQPSYWSQIKSRSRLIGERLARQFEQCCQKPVGWMDTEHAAGATPAVATAPAPAD
                     DPLPQDDDERFIVGLVLTYYRRHPQRARTRLLDLLGEVLMPPAEPAAPAQPPAAAPRK
                     LAPGVTARPPAPPAPPDDPMALWHSAQAGIAPLKKKR"
     gene            1464..1589
                     /locus_tag="VIN75_25805"
     CDS             1464..1589
                     /locus_tag="VIN75_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977656.1"
                     /translation="MRQALNRSRTATAAVRTALRGARAARFPAARNAAKPLPIPL"
     gene            1681..1869
                     /locus_tag="VIN75_25810"
     CDS             1681..1869
                     /locus_tag="VIN75_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977657.1"
                     /translation="MSKLIVPTARPRNPFVAAARFRQAGQHGGGQGAERREGERALKR
                     ELSQLDLPRDVRGKPPSL"
     gene            1909..2175
                     /locus_tag="VIN75_25815"
     CDS             1909..2175
                     /locus_tag="VIN75_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977658.1"
                     /translation="MTVLTGHRATQRASFSDTWRRLVAHLLRGASAALARQARDVARP
                     LHARQRRVRSQHDASVEFHAEAGAPEGALYVDGELVGYIDVRRL"
BASE COUNT          313 a          860 c          750 g          277 t
ORIGIN      
        1 agcgccgcgc ccgtgtcggg gcggatctgg aacagcccgc ggctcgcgac gtcggccggc
       61 ggcgcggcca tgcgcaccag cgcgaggtcg tgcacgacgt cgaacgcgac gagctgcagc
      121 gcgccggtga cgccgtcgac cgtctggaag ttcagccggt agcgctgcgg ctcgagggcc
      181 acctggctga ccacgtggta gttggtcagg atcaggccgc cggggcccac cagcgagccg
      241 gagcccacgc tggcctggct gtcctggtcg cgcaacagcg tgcgcacctg caataggcgc
      301 ggtcggccga cgtcgtagat gcggcgcgcg gaggccgaca ggttggcgct gtcgtcgtgc
      361 tcgtcctcct gcgcggccgc ggatgcggcg gccggcggct tgacgagcgg cgaggtgggc
      421 gcgagcgtcg gccgtgcgtc cgagggcgtg gcgccggccc aggccgcgag cgtggccccg
      481 cccagcagca gggcggcgag aaggcgtccg ggttgccgga gagggcggtt caggagcatc
      541 gacccatcct accccggccc tggcgtgcgg cattgccggg ggacggccgg cgcgacggac
      601 ggccgatccg gcggcctgcg ttacgagcct ttatcattac aaaccatgca cgccccccga
      661 gacgtggcct ctatccgcct ggacaacgcc ctcgcgctgt tcgacgaatt cgtccgcaac
      721 accatcaagc accccgacgc cgcgacgctg cggggcctgg aacgccgttt cgccgaacgg
      781 ctgcagatcc agccgagcta ctggagccag atcaagagcc gttcgcgcct gatcggcgag
      841 cggctcgcgc ggcagttcga gcagtgctgc cagaagccgg tcggctggat ggacaccgag
      901 cacgccgccg gcgccacgcc ggccgtggcc acggcgcccg cgccggccga cgatccgctg
      961 ccgcaggacg acgacgagcg cttcatcgtc ggcctcgtgc tgacctacta ccgccgccac
     1021 ccgcagcgcg cccgaacgcg gctgctcgac ctgctcggcg aggtgctgat gccgccagcc
     1081 gagccggccg cgcccgcgca gccccccgcc gccgcgccgc gcaagctggc gcccggtgtc
     1141 acggcgcgcc cgccggcgcc gcccgcgccg ccggatgacc cgatggcgct gtggcactcc
     1201 gcccaggccg gcatcgcgcc gctcaagaag aagcgctgag cggcgcgccc gcgcccgcct
     1261 ttcccgcccg gcgcgcgggc gccgcgatgg gggtgcggtc gtgcgcgcct gaccacgtcc
     1321 gacgccctcg cgccacgaac tctgatcaat tgattgataa agtacttgcg caagatcaag
     1381 agttgtttat cattgcgtca agcgttcagt cacggcatcg cctcctgacg cgacaccccg
     1441 cctcgcaacc cgtcacgagc cccatgcgcc aagccctgaa ccgatcccgc accgccaccg
     1501 cagccgttcg cacggccctg cgcggcgcgc gcgccgctcg tttcccggcg gcccgcaatg
     1561 cggccaagcc gttgccgatt ccgctctgag ttcgcgagac cctctcttcg cgaaggcccg
     1621 ggcggttgtt cagacccccg ggccttcgcc attccaggcc gcccaccgca ggagaaaacc
     1681 atgtccaagc tcatcgtccc gaccgcccga ccacgcaatc cgttcgtcgc cgccgcgcgc
     1741 ttccggcaag ccggacagca cggcggcggc cagggcgccg aacgccgaga aggcgaacgg
     1801 gcgctcaaac gcgaactgtc ccagctcgac ctgccccgcg acgtgcgcgg caagccgccc
     1861 agcctctgaa tcctcgcaag cggccgcagc gccgccctgg agaacgacat gaccgtcctg
     1921 accggccacc gcgccacgca gcgggcctcc ttcagcgata cctggcgccg tctcgtggcg
     1981 catctcctgc gcggcgccag tgccgcgctc gcccgccagg cgcgcgacgt cgcccggccc
     2041 ctgcacgcgc gccagcgccg cgtgcgcagc cagcacgacg ccagcgtcga gttccatgcg
     2101 gaggccggcg cccccgaagg cgcgctgtac gtcgatggcg aactcgtcgg gtacatcgac
     2161 gtccgccgcc tgtgatccgc gccttggccg ggcgcgcccg
//
LOCUS       DATCRO010000945        11861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_134796, whole genome shotgun sequence.
ACCESSION   DATCRO010000945 DATCRO010000000
VERSION     DATCRO010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134796"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VIN75_25820"
     CDS             complement(<1..1223)
                     /locus_tag="VIN75_25820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8977659.1"
                     /translation="MRIAMISEHASPLAVIGGVDSGGQNIYVAHMARCLVELGHEVDV
                     FTRCDDAALPPVVEMAPGLHVVHVTAGPERFVPKEELLGHMPEFARFCISWMRVVPRY
                     DVVHANFFMSGFVGLRLQEAFDLPLVTTFHALGLVRREHQGDADAFPPARIGIERLLA
                     AHSDRVVAECPQDHQDLVRLYDADARRIAMVPCGFDPGEFAPMPRAQARAALGIPDDE
                     FMVLQLGRMVPRKGIETVVRAMAHAGCEAMRLHVVGGDCTPADPARTPEIGRLMAVAR
                     DCGVGDRVVFEGRRPRDELHRWYGAADVFVTTPWYEPFGITPLEAMACARPVVGSAVG
                     GVRYTVVDGSTGLLVPPKDERALARALARLRAEPARARAMGEAGLQRVRAEFTWARVA
                     ASLAGVFEDAVRARAR"
     gene            complement(1201..1815)
                     /locus_tag="VIN75_25825"
     CDS             complement(1201..1815)
                     /locus_tag="VIN75_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEY8977660.1"
                     /translation="MTTALLHRYLAGHRVVFDTIEGLAGEIATVAERLAQCLRDGGKI
                     LLCGNGGSAADCQHLAAEFTGRFNEERAPLAALALTTDSSALTCIGNDYGFEHVFARQ
                     VQALGRPGDCLVGISTSGRSVNVLRAMTLARERGLSTIGLLGRDGGDIAPLCDLAVIV
                     PGTDTARLQEAHLFIEHAWCALVEHALSEQPPKQGSLHAHRHDQ"
     gene            complement(1812..2828)
                     /gene="rfaE1"
                     /locus_tag="VIN75_25830"
     CDS             complement(1812..2828)
                     /gene="rfaE1"
                     /locus_tag="VIN75_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY8977661.1"
                     /translation="MTPPAARPILVVGDSMLDRYWEGRVDRLSPEAPVPILRVTSESS
                     RVGGAGNVALNVAALGAPVTLLTLVGADEAGDVLSGLLRRGGVTLHAVGGHRHRTTQK
                     IRCVAQRHQLLRTDFEDLATDDCCRALLQQFSELLPERAIVVLSDYAKGALRDCPEMI
                     ALARSRGCTVLVDPKGSDFRRYRHADLVKPNAAELRAVVGDWRDERELQRKCASLRRR
                     LALGTLMVTRGEEGLSLFGAAGARHHRAEAREVFDVCGAGDTVIAALARFLAAGCPLD
                     DSARWANRAAGVAVSRFGTTAVTLAELGLSSVPSHEDADAGERAQAHGPRRRAAASHA
                     EYAQ"
     gene            complement(2825..3352)
                     /locus_tag="VIN75_25835"
     CDS             complement(2825..3352)
                     /locus_tag="VIN75_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY8977662.1"
                     /translation="MPFPVVVADDDAAPFSLDAALSRWALLPRPRVFTNGVFDLLHRG
                     HCGLLREARALGGSLVVAVNSDASARRLGKGAGRPFNTAFDRASVLAALRDVDLVVTF
                     DEDTPCELLARLRPELYVKGGDYDVARLPEAALVSGWGGRALALRYQAGLSTTGIVER
                     IAAAHPASQLEGAVA"
     gene            3730..4206
                     /locus_tag="VIN75_25840"
     CDS             3730..4206
                     /locus_tag="VIN75_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY8977663.1"
                     /translation="MDKDTTWIVLADEGRARILSREADGGDLRELEVLDDPAAHAHNA
                     DFRHDAYGRRAPAGAAQPASATSSAGEEKLDHEAELFARRLALRLAEAHRQHRFAHLR
                     IAAAPRFLGRLRQALAREVRDVVDEEIDRDLLQLDTRALTQRFYGAATQPAPPTRH"
     gene            4256..4331
                     /locus_tag="VIN75_25845"
     tRNA            4256..4331
                     /locus_tag="VIN75_25845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4289..4291,aa:Ala,seq:cgc)
     gene            complement(4523..4819)
                     /locus_tag="VIN75_25850"
     CDS             complement(4523..4819)
                     /locus_tag="VIN75_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977664.1"
                     /translation="MTLFTMTSRPTHREPAARYAPDRHALLKECGIQIDDVVSVIDSR
                     GGAAYPGSVEVGLANRFPHVEESERQRAVARALAKRAIVMTARGVLEPWRGQGD"
     gene            complement(4890..5147)
                     /locus_tag="VIN75_25855"
     CDS             complement(4890..5147)
                     /locus_tag="VIN75_25855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977665.1"
                     /translation="MHAECFRVKVPARSGLAGFTAGGQRVPVMPGEYVVHRVMPKVPV
                     AGIAETLRFLGADPHGRDLHVPVERGGDVHAVLKGAQALSD"
     gene            complement(5168..5479)
                     /locus_tag="VIN75_25860"
     CDS             complement(5168..5479)
                     /locus_tag="VIN75_25860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8977666.1"
                     /translation="MDIFEDAGGITLLADMPGVSRDHLDVRLDGDSLAIEGDVDITMP
                     DGMRALWAEVNVPRFRRTFTLSRELDASRIEANLKDGVLSLRIPKLAHAQPRRITVQT
                     G"
     gene            complement(5620..6072)
                     /locus_tag="VIN75_25865"
     CDS             complement(5620..6072)
                     /locus_tag="VIN75_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8977667.1"
                     /translation="MLETMFYRPQGLFDQLNSLHQALTRSLAADGLPDAIRSVGAGSY
                     PGVNVGRTAKTVEVYAFAPGLDAASIDVTVERGVLKISGERRAADQGKDAKVQAYAAE
                     RPVGRFSRGLSLPDDVDTSKVEAKYRDGVLQVSIGLLESAQPQRIKVQ"
     gene            7006..8502
                     /locus_tag="VIN75_25870"
     CDS             7006..8502
                     /locus_tag="VIN75_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY8977668.1"
                     /translation="MSALVDPPTDRISTGVEGLDAILRGGLTPSRMYLLEGAPGTGKT
                     TFGLRFLMAGVAAGETGLYITLSETTAELAAVVESHGWTLEGIQVHELGHDVGLMAEA
                     QQTVLHPSEIELGETIRSMTEVIERLDPRRVVFDSLSEMRLLAQDPLRYRRQILALKQ
                     FFSGRPCTVLLLDDKTSEPADLQLHSIAHGVISLEQDAGKFGAERRALRVVKMRGLKF
                     EGGWHDFMVDTGVLEVFPRLISARHRKAFDIPLASTGLDSLDAMLGGGLTAGTSMLLL
                     GPSGAGKTTTAVKCMLSALQRGETADYYLFDEGKATLLARSRSLGMALEDYIESGRLI
                     VHQVDPASLSPGEFASRVVNGVKTRKVSFVGIDSLNAYVHAMPGQSFLTLHMHELLTF
                     LNQQGVLTMLIVGQHGLVGDVRSDIDLSYLSDSILLYRFFEADSQVRTAISVLKSRTG
                     QNQRSIRELRLGKPAGIQVGDELRGFNGVLAGAVAYTGSTPMLSDSKG"
     gene            8508..10202
                     /locus_tag="VIN75_25875"
     CDS             8508..10202
                     /locus_tag="VIN75_25875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977669.1"
                     /translation="MKLDKDKRLALILAPRGRDAAVIEQVLAPLETRFKRCANLTELT
                     RCLCDAATMAIVTEEALRDEGLRSLQAWIEHQPPWSDFPFVVLASREATRRSPEALAG
                     LGNDVLLERPLSADTLIKAVSAAMRSRRRQYEAREQLQQYEQIGLENRRLYESESRAR
                     SEAEAANRAKDEFLATLSHELRTPLSAILGWTYVLQQRRSELGDLARGIDTIQRNALS
                     QARLIEDLLDMSRIVAGKVSLEVQTVVPAMAIDQVVASLLPTAQAKNIEIRTDLEAAM
                     TPISGDAQRLQQVFRNLLVNALKFTPPGGRVEVRTAVREAQLLVTISDNGAGIAPDFL
                     PHVFERFRQADGSTTRSQGGLGLGLAIVQRLVDLHGGTVSAASEGIGKGATFEVRLPL
                     VGSDPSGTGTSAPMGDYPALASSQDAPIAGLQVLLVEDDPDGREMVERLLRDNGAQVT
                     AVESAEHALWALRERDLDVMISDIAMPRVDGYELLARARQEGFGLPAVALTAFARPED
                     KARALEVGYSSHVSKPVEPMALINVVAAAARNRDIEVRAPGSHDGPHAVTSASRDQ"
     gene            complement(10288..10905)
                     /locus_tag="VIN75_25880"
     CDS             complement(10288..10905)
                     /locus_tag="VIN75_25880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977670.1"
                     /translation="MSRVPILSGWLRPARLLGEGLKRMVLRYSARKRTGGKPEAASFT
                     DTRFATSSVARSTAAPASLLAPMDPDRLRDARQSMRRILDCHPAAPAIWPALALVDRA
                     LARHGALGLERLSAQVLQDAARVLNRLMDELCEHGIVVLLERIDRVLRVVHGCAIAAE
                     SQWLAVRPDEVQVLESTITEFMEIERALEEQRAAGQDDDRRALAA"
     gene            11042..>11861
                     /locus_tag="VIN75_25885"
     CDS             11042..>11861
                     /locus_tag="VIN75_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8977671.1"
                     /translation="MTATAPARRRGHGDRHIRQAWTLRCLYTKIAFNPWMHRTGADAP
                     YLRMPGTTSVSEPSSAKKIANHVVDAILSGRVTPGQRLGEQELADVFGVSRTLVREAM
                     ARLSARGMVEVTSRRGWFVVQPSRAEATEAFSARAAIETGMLGSLGGPLARRKIARLK
                     THCKAEQAAVSAGDVGSRSFLLGDFHVCLAECVSGPMLADILRDLTARTTLVATLFQS
                     THDAAQSCGEHVAIVAALERGDPAGAAELMRAHIGNVAAALGSLGGPLARRKIAR"
BASE COUNT         1880 a         4099 c         4129 g         1753 t
ORIGIN      
        1 cgggcgcgcg cgcgcaccgc gtcctcgaac acgccggcca ggctcgccgc gacgcgcgcc
       61 caggtgaatt cggcgcgcac gcgctgcagg ccggcctcgc ccatcgcccg tgcgcgcgca
      121 ggctcggcgc gcagccgcgc cagtgcgcgg gcgagcgcgc gttcgtcctt gggcggcacc
      181 agcagcccgg tgctgccgtc gacgaccgta tagcgcacgc cacccaccgc gctgccgacg
      241 acgggccgcg cgcacgccat cgcctcgagc ggcgtgatgc cgaacggctc ataccacggc
      301 gtcgtcacga agacgtcggc ggcgccatac cagcggtgca gctcgtcgcg cggccggcgg
      361 ccctcgaaca cgacgcggtc gccgacgccg cagtcgcgcg ccacggccat caggcggccg
      421 atctccggcg tgcgcgccgg atcggccggg gtgcagtcgc cgccgaccac gtgcagccgc
      481 atcgcctcgc agcccgcatg ggccatcgcg cgcaccaccg tctcgatgcc cttgcgcggc
      541 accatgcggc ccagctgcag caccatgaac tcgtcgtccg ggatgccgag cgcggcgcga
      601 gcctgcgcgc gcggcatcgg cgcgaactcg ccggggtcga agccgcaggg caccatcgcg
      661 atgcggcgcg catccgcgtc gtacagccgc accaggtcct ggtgatcctg cgggcattcc
      721 gcgacgacgc ggtcgctgtg cgcggccagc aggcgctcga tgccgatgcg cgccggcggg
      781 aacgcatcgg cgtcgccctg gtgctcgcgt cgcaccaggc ccagcgcgtg gaacgtggtg
      841 accagcggca ggtcgaacgc ctcctgcagc cgcagcccga cgaagccgga catgaagaag
      901 ttcgcgtgca cgacgtcgta gcgcggcacc acgcgcatcc agctgatgca gaagcgcgcg
      961 aattcgggca tgtgcccgag cagttcctcc ttgggcacga agcgctcggg gccggccgtg
     1021 acgtgcacga cgtgcagccc cggcgccatc tcgacgacgg gcggcagcgc ggcgtcgtcg
     1081 cagcgcgtga acacgtcgac ctcgtggccg agctcgacga ggcagcgcgc catgtgcgcc
     1141 acgtagatgt tctggccgcc cgagtccacg ccgccgatga cggccagcgg ggaggcgtgc
     1201 tcactgatca tggcgatgcg catgaaggga tccttgcttg ggaggttgtt cgctcaatgc
     1261 atgctcgacc agcgcgcacc acgcgtgctc gatgaacagg tgcgcttcct gcaggcgcgc
     1321 cgtgtcggtg cccggcacga tcaccgcgag gtcgcacagc ggcgcgatgt cgccgccgtc
     1381 gcggcccagc aggccgatgg tcgacaggcc gcgctcccgc gccagggtca tggcgcgcag
     1441 cacgttgacc gagcggcccg aggtcgagat cccgaccagg cagtcgcccg gccgcccgag
     1501 cgcctgcacc tggcgcgcga agacgtgctc gaagccgtag tcgttgccga tgcaggtcag
     1561 cgccgagctg tcggtggtca gcgcgagcgc cgccagcggc gctcgctctt cgttgaagcg
     1621 gccggtgaat tcggcagcca gatgctggca gtcggccgcg gagccgccgt tgccgcacag
     1681 caggatcttg ccgccgtcgc gcaggcattg cgccagccgc tcggcgacgg tcgcgatctc
     1741 gccggcgagg ccttcaatcg tgtcgaagac gacccgatgc cccgccaggt atcgatgcag
     1801 cagggccgtg gtcattgcgc gtactccgca tggctggccg cagcgcggcg acggggcccg
     1861 tgcgcctgcg ctcgctcgcc ggcgtccgcg tcctcgtgcg acggcacgct cgacaggccg
     1921 agctccgcca gcgtcacggc cgtcgtgccg aagcgcgaga ccgcgacgcc cgcggcccgg
     1981 ttggcccagc gggcgctgtc gtccagcgga cagcccgccg ccaggaagcg ggccagcgcg
     2041 gcaatgacgg tgtcgcccgc gccgcagaca tcgaacacct cgcgcgcctc ggcacgatga
     2101 tggcgcgcgc cggcggcccc gaacaggctc aggccctcct cgccgcgcgt gaccatcagc
     2161 gtgccgagcg ccagccgccg gcgcaggctc gcgcacttgc gttgcagctc gcgctcgtcg
     2221 cgccagtcgc ccaccaccgc ccgcagctcg gccgcgttcg gcttgaccag gtcggcatgg
     2281 cggtagcgcc ggaagtcgct gcccttgggg tcgaccagca ccgtgcagcc gcgcgaccgc
     2341 gccagcgcga tcatctcggg gcagtcgcgc aatgccccct tggcgtagtc cgacagcacg
     2401 acgatcgcgc gctcgggcag cagctcggag aactgctgca gcagcgcgcg gcagcagtcg
     2461 tccgtggcca ggtcctcgaa gtcggtgcgc agcagctggt ggcgctgcgc gacgcagcgg
     2521 atcttctgcg tcgtgcggtg gcgatggccg ccgacggcat gcagcgtcac gccgccacgg
     2581 cgcagcaggc cggacaggac gtcgccggcc tcgtcggcgc ccacgagcgt gagcagggtc
     2641 accggcgcgc cgagggccgc gacgttcagc gccacgttgc ccgcgccacc gacgcggctg
     2701 gactcgctgg tgacgcgcag gatgggcacc ggcgcctcgg gcgacagccg gtcgacccgg
     2761 ccctcccagt agcggtcgag catcgagtcg cccaccacca ggatggggcg cgctgccggc
     2821 ggcgtcatgc gacggctcct tcaagctgcg acgcgggatg cgccgccgcg atgcgctcga
     2881 cgatgccggt ggtcgacagc cccgcctggt agcgcagcgc cagcgcgcgg ccaccccagc
     2941 cggacaccag cgcggcctcg ggcaggcgcg cgacgtcgta gtcgccgccc ttgacgtaga
     3001 gctcgggccg caggcgtgcg agcagctcgc acggcgtgtc ctcgtcgaac gtcacgacca
     3061 gatcgacgtc gcgcagggcc gcgagcacgc tcgcgcggtc gaaggccgtg ttgaacgggc
     3121 ggcccgcgcc cttgcccagc cggcgcgcgg acgcatcgga attgaccgcg accaccagcg
     3181 acccgccgag cgcgcgcgcc tcgcgcagca ggccgcagtg cccgcggtgc agcaggtcga
     3241 acacgccgtt ggtgaacacg cgcggacgcg gcagcagggc ccaccgcgac agcgccgcgt
     3301 ccagcgagaa cggggcggca tcgtcatccg cgacgacgac aggaaagggc attcgactca
     3361 ctcggtgtgc tccgggcgcg accatcgcgc ggagcctctc tttgcaagca tggtgccgtt
     3421 cgtccctccg cggtgcgaag accaggcgcg ggcgagacgc ttctcatagg tgagaagcgc
     3481 ttgccggacg ccaccctcga ccgttttccg ccgcccgggc tccgtcgcgc agcaggccaa
     3541 aggaacttca aacgtctgca acaagctgca tgagcttgcc gatgcggact ggatggatcc
     3601 gcagcgatga acgaccgtcc aggccatatg gaatgggcga tccgtccata ccttgacggg
     3661 tatctcgacc gtgcgtgcgc ggcacccggg ttgccgcgag cgaccatgcc aatcgacaag
     3721 gagcccacga tggacaagga cacgacctgg atcgtgctgg ccgacgaagg ccgcgcccgc
     3781 atcctgagcc gcgaggccga cggcggcgat ctgcgcgagc tggaggtgct ggacgacccc
     3841 gccgcgcacg cgcacaacgc cgatttccgc cacgacgcct acggccggcg cgcgcccgcc
     3901 ggcgccgccc agcccgccag cgcgaccagc tcggccggcg aggagaagct cgaccacgag
     3961 gccgagctgt tcgcgcgccg cctcgcgctg cgcctggccg aggcgcaccg ccaacaccgc
     4021 ttcgcgcatc tgcgcatcgc cgccgccccg cgcttcctcg ggcgcctgcg ccaggccctc
     4081 gcgcgcgagg tccgcgacgt ggtcgacgag gagatcgacc gcgacctgct gcagctcgac
     4141 acccgtgccc tgacccagcg cttctacggc gccgccaccc agcctgcgcc accgacccgg
     4201 cactgacgcc cgaccgccgg acggcaggaa atgtctgtta tagtctcggg ctgttggggg
     4261 tgtagctcag ctgggagagc gtcgcgttcg caatgcgaag gtcaggagtt cgatcctcct
     4321 catctccacc aacgaattca aggtcttggc attcgcgtga cttcgcgcat tcgattgcgc
     4381 cgaatgcaag cgtcaggttt atccagacgg tccaaggcct gcgtccaaga gaacggctcc
     4441 ctcgggagcc gttttttctt tgcggctgaa ctcggaaaga cctgctcact cagcgggtaa
     4501 tgcgccgggc cagcgggcaa ggtcagtcgc cctggcctct ccatggttcg agcacgccgc
     4561 gtgccgtcat gacgatggcc cgcttggcaa gggcccgcgc caccgcgcgt tggcgctcgg
     4621 attcctcgac gtgcgggaac cggttcgcca ggcccacctc gacggagcct ggataggcgg
     4681 cgcctccccg cgagtcgatg acggagacga cgtcgtcgat ctggatgccg cactccttca
     4741 ggagcgcatg ccggtccgga gcgtagcgag ccgcaggctc gcgatgtgtc gggcggctgg
     4801 tcatggtgaa caaggtcaac aggcgatacg gcctgggaac gtgcgacgat ttcggtggat
     4861 tgaatgccgg cgccagtggg cgccggcatt cagtcggaca gcgcttgagc gcctttcagg
     4921 acggcatgga cgtcgccgcc gcgctccacg ggaacgtgaa ggtcgcgccc gtggggatcc
     4981 gccccgagaa agcgcaaggt ttccgcgatg ccggcgacgg gcaccttggg catcacccgg
     5041 tggacaacgt actcacccgg catgaccggg acgcgttgac cgccggcggt gaagccggcg
     5101 agaccgctgc gggcgggaac cttgacgcga aagcactccg cgtgcatggc atcgctcctt
     5161 cgcgtgctca gcccgtctgc acggtgattc gccgcggttg ggcgtgcgcc agcttcggga
     5221 tgcgcagcga gaggacgcca tccttcagat tggcctcgat gcggctggcg tcgagctcgc
     5281 ggctgagcgt gaacgttcgg cggaagcgcg gcacgttcac ttcggcccac agcgctcgca
     5341 tgccgtccgg catcgtgatg tcgacgtcgc cttcgatggc gagggaatcg ccgtcgaggc
     5401 gtacatcgag atggtcgcgg gagacaccgg gcatgtcggc cagcagcgtg atgccgccgg
     5461 catcctcgaa gatgtccacg gcgggcacgg ccgcgggccg ggcgccggac tcctggcggc
     5521 cggagtcgcc ttgcgatacc tcggaacggc cacgttgggg cgtggccagg tcggtcttgg
     5581 acatgacgtt ttcctttcct ggtcgttgag ggttgaggat cactgcacct tgatgcgctg
     5641 cggctgggcg gattccagca ggccgatgct gacctgcagg acgccatcgc gatacttcgc
     5701 ctccaccttg gacgtgtcga cgtcgtcggg cagcgacaga ccgcgcgaga agcgtcccac
     5761 gggccgttcg gccgcatagg cctgcacctt cgcatccttg ccctggtcgg cagcacggcg
     5821 ctcgcccgag atcttgagca cgccgcgctc cacggtcacg tcgatcgagg cggcgtcgag
     5881 gccgggggcg aacgcgtaga cttccacggt cttggccgtc cgaccgacgt tgaccccggg
     5941 gtagctgccg gcaccgaccg aacgaatcgc gtcgggcagg ccatcggcgg ccagtgaccg
     6001 cgtcaaggcc tggtgaaggc tgttgagctg gtcgaagagc ccttgagggc ggtagaacat
     6061 cgtttcgagc atgttgaacc tccgagtcga gggtgtgatg ccgaatgcgg ggcatcgccg
     6121 cttgtgcccg cctgtgggca cccagccgcg accacgggcg cggctgtacc gaggccagga
     6181 aagcctcacg aatttgcggc agggaatgga atcagcctgg cggagcggaa ccggcagtca
     6241 gcacgtccct ggcgacgcac ggcgtcgggg acggcttgag acggcggatc gtctctcgtg
     6301 ccatgaagta ggcctgccat tgcaacgcgc tcaggggcgc ggcaggccgt cgtgacgtac
     6361 gtgcggcaga cttgccgctg cgagggcggt atgaaggatt ggccatgatt cagtcctcat
     6421 ccgaatcggt cgttggcaaa ttgacggtgt acggaatcta ggttcggaac caggcgattc
     6481 aagttccccg ggataagtga aagccctgac cttgaagcgc ctgcccggag agccgatccg
     6541 gcgcccacca ggagggccgg ccggacttgc gcgggacctg tcagggcccc tggcttgcat
     6601 cccggcaggg gcgcagttgt gccagttgtc ggaggcccgg caccgggtgc gcacgcctga
     6661 agatctcccg tgccaggaaa tagtccagtt gacgggacga cggttccggc gtcgacgcgg
     6721 accggcgccg gcgggtgtcc cgggtcggtg tctctgatgg tcgggccatg actcagttcc
     6781 tcatccgaat ctttgcggct ggtgccatgc aaattatcta ggcatcgcct gcaccgtttc
     6841 aaggcccccg ccagagccgc cccggtgaag ggctgcccat gtcagaggcc gccgacaggt
     6901 acggaggttg ctctaccgcc agcggccgcg gggcgcgcga ctcccgtcca cgcgccactc
     6961 cttcgatcgt gtttccgggc acctcgcccg gaggaggcgt gttgcatgtc tgcacttgtc
     7021 gatccaccca cggaccggat ctcgacgggc gtggaaggcc tggatgcgat tcttcgcggc
     7081 ggcctgacgc cgtcccggat gtacctgctg gagggtgccc ccggcaccgg caagacgacc
     7141 ttcggcctgc gattcctgat ggcaggcgtc gccgccggcg agaccggcct gtacatcacg
     7201 ctttccgaga ccacggccga actggctgcg gtcgtggagt cgcacggctg gacgctggaa
     7261 ggaatacagg tccatgaact cggacatgac gtcgggctca tggccgaggc gcagcaaacc
     7321 gttctgcatc catcggagat cgagcttggc gaaaccatcc gatcaatgac ggaggtcatc
     7381 gaacggctcg atccgaggcg cgtcgtcttc gacagccttt ccgagatgcg tctgctcgcc
     7441 caggatccgc tgcgctatcg acggcagatc ctggcattga agcaattctt ctcgggacgc
     7501 ccgtgcacgg tgctcctgct ggatgacaag acctccgaac cggcggacct gcaactgcac
     7561 agcattgctc acggcgtcat ctcgctcgag caggatgccg gcaaattcgg agccgagcgg
     7621 cgagccctgc gtgtcgtcaa gatgcgcggg ctcaagttcg aaggcgggtg gcacgacttc
     7681 atggtcgaca ccggcgtcct cgaggtattc ccgcgtctga tttcggcgcg gcaccgcaag
     7741 gcgttcgaca tccccctcgc gtcgacggga ctggactcgc tggacgcgat gctgggcgga
     7801 ggcctcaccg ccggaacgag catgctgttg ctcggcccat cgggcgccgg caagaccacg
     7861 accgcggtca agtgcatgct gtcggccctg caacgcggcg agacggcgga ctactacctg
     7921 ttcgacgagg gcaaggcgac gctgctcgcg cgcagccgca gcctcggcat ggcgctggag
     7981 gactacatcg agtcaggccg cttgatcgtc catcaggtcg acccggcgtc gctgtcgccg
     8041 ggagagttcg cgtcgcgcgt ggtgaacggg gtcaagacgc gcaaggtcag tttcgtgggt
     8101 atcgacagcc tcaacgccta cgtgcatgcg atgcctggcc agagcttcct gaccctgcac
     8161 atgcacgaac tgctcacgtt cctgaaccag cagggcgtgc tgaccatgct gatcgtcggc
     8221 cagcacgggc tggtcgggga cgtgcgatcc gacatcgacc tgagctacct gagcgattcg
     8281 atcctgttgt atcgcttctt cgaagcggac agccaggtcc gcacggccat ctccgtgctc
     8341 aagagccgca cgggacagaa ccagcgctcg attcgcgaac tgcgcctggg caagccggcc
     8401 gggatccagg tcggcgacga actgcggggg ttcaatggcg tgctggccgg cgcggtcgcc
     8461 tataccgggt ccaccccgat gctgtcggac tcgaagggct gagcgtgttg aagctggaca
     8521 aggacaagcg gctcgcactg atcctcgctc cgcgaggcag ggatgcagcg gtcatcgagc
     8581 aggttctcgc gccgctcgag acacgtttca agcgctgcgc gaacctgacg gagctgaccc
     8641 gctgcctgtg cgacgcggcg acgatggcga tcgtgacgga ggaggcgctg cgagatgaag
     8701 gcctgagatc gctccaggcc tggatcgagc accagccgcc atggtccgac tttccgttcg
     8761 tcgtgctcgc aagccgggag gcgacgcggc ggtcgccgga ggcgttggcc ggcctgggca
     8821 acgacgtgct cctggagcgc ccgctcagtg cggacacgtt gatcaaggcg gtcagcgccg
     8881 ccatgcggtc gaggcggcgc cagtacgagg cccgcgagca actgcagcaa tacgagcaga
     8941 tcggcctcga gaaccgcagg ctctacgaat ccgagagccg cgcgcgatcg gaggcagagg
     9001 cggccaaccg ggcgaaggac gagttcctcg ccaccttgtc gcacgaactc cggacgccgc
     9061 tcagtgccat cctcggatgg acctatgtcc ttcagcagcg ccgcagcgaa ctgggagacc
     9121 tggcgcgtgg gatcgacacg atccagcgca acgccctctc ccaggcccgg ctgatcgagg
     9181 acctgctgga catgagccgc atcgtcgccg gcaaggtgag cctggaagtg cagacggtcg
     9241 tgcccgccat ggcgatcgac caggtcgtcg catcgctgct tcccacggcg caggccaaga
     9301 acatcgagat ccggaccgat ctggaggccg cgatgactcc gatcagcggc gacgcgcagc
     9361 gactgcagca ggtctttcgc aacctgctgg tcaatgcact gaagttcacg cccccgggcg
     9421 ggcgcgtgga ggtccggacg gctgtcaggg aagcgcagtt gctggtcacc atcagcgaca
     9481 acggtgccgg catcgcgccg gattttcttc cccacgtgtt cgagcgcttc cgccaggccg
     9541 acggatcgac gacccgatcg cagggcggcc tcggcctggg cctggcgatc gtgcaacgcc
     9601 tcgtggacct tcacgggggc acggtcagcg ccgcgagcga aggaatcggc aagggcgcca
     9661 cgttcgaggt gcgactgcct ctggtcggca gcgatccgag cggcacagga acgtctgcgc
     9721 cgatgggcga ctacccggca cttgcttcgt cgcaggacgc gccgatcgcg ggcctgcagg
     9781 tgttgctggt cgaggacgat cccgacggtc gcgagatggt cgagcgactg cttcgcgaca
     9841 acggtgcgca ggtcaccgcg gtcgaatccg cagagcacgc actgtgggcg cttcgcgaac
     9901 gcgatctgga cgtgatgatc agcgatatcg ccatgccacg tgtggacggc tacgaacttc
     9961 tcgctcgagc caggcaggaa ggattcggct tgccggccgt ggcgctgacg gcgttcgccc
    10021 ggcccgagga caaggccagg gctctcgagg tcggctactc gtcccacgtc agcaagccgg
    10081 tcgagccgat ggcgctgatc aacgtggtcg cggcagcggc ccgcaatcga gacatcgaag
    10141 tcagggcccc tggatcgcac gacggcccgc atgccgtgac cagcgcttcg cgcgatcagt
    10201 gagtcgctgc gtcaactgcg cggggcatgg accattgctt cgtcgccgcc gcgggcgatg
    10261 cccccatgcc gcggcggcgc gcgtccatca tgcggcgagc gcccggcggt cgtcgtcctg
    10321 gccggcagcc cgttgctctt ccagcgcgcg ctcgatctcc atgaactcgg tgatcgtgga
    10381 ctcgagcacc tgcacctcgt cggggcgcac ggccagccac tgcgattcgg cggcgatcgc
    10441 gcagccgtgg acgacccgca gcacgcggtc gatgcgttcc agcagcacga cgatgccgtg
    10501 ttcgcagagt tcatccatca accggttcag aacgcgggcc gcgtcctgca gcacctgggc
    10561 cgaaaggcgc tcgagcccga gggcgccatg tcgcgccaac gcgcgatcga cgagcgcgag
    10621 tgccggccag atcgcgggcg ccgcgggatg gcagtccagg atgcggcgca tgctctgacg
    10681 agcgtcgcgc aggcgatccg gatccatggg agcgagcagg ctcgccggag cggccgtgct
    10741 gcgcgccacc gacgatgtcg cgaacctcgt gtcggtgaac gacgccgcct ccggcttgcc
    10801 accggtgcgc ttccgggcgg agtaccgcag caccatccgt ttcaggccct cccccagcag
    10861 gcgggccggc cggagccacc cggacaggat cgggacgcgg ctcatgggtc tggcgactgg
    10921 gcgggattgg gtaggcatcg agggactcac ggtggacgca tgcgcgacac gaaagccgtg
    10981 cggctgcgat cgagcttagc gatcccgcga cccaaggtca tgacggcggc gcgatcaatc
    11041 gatgaccgcg accgccccgg cacgccggcg tggccacggc gaccggcaca tccgccaggc
    11101 atggaccttg cgttgcttgt atacaaagat cgcattcaat ccttggatgc accgcaccgg
    11161 ggccgacgcc ccatacctgc gcatgccggg caccacatcc gtttccgaac cttcgagcgc
    11221 gaagaagatc gccaaccacg tcgtcgacgc catcctgtcg ggacgcgtca cgccgggtca
    11281 gcgcctggga gagcaggagc ttgccgacgt cttcggcgtc agccggacgc tggttcgcga
    11341 ggcgatggca cggctgtccg cgcgtgggat ggtggaagtc accagccggc gcggctggtt
    11401 cgtggtgcag ccctcgcggg cggaggcgac ggaagcgttt tccgcgcgcg ccgcgatcga
    11461 gaccgggatg ctcggctcgc tgggcggccc cctcgcgcgc cgaaagattg cccgcctgaa
    11521 gacgcattgc aaggccgaac aggcggccgt ctcggccggc gacgtgggaa gccgcagctt
    11581 tctcctcggc gatttccatg tctgtctggc cgaatgcgtg agcggcccga tgctggcgga
    11641 catcctgcgc gacctcaccg cgcgcaccac tctcgtggcc acgctcttcc aatcgacgca
    11701 cgatgccgcg cagtcctgtg gcgagcacgt ggcgatcgtc gcggcgctcg aacgcggcga
    11761 cccggccggc gcggccgaac tgatgcgggc gcacatcggc aacgtcgcgg ccgcgctcgg
    11821 ctcgctgggc ggccccctcg cgcgccgaaa gattgcccgc c
//
LOCUS       DATCRO010000946         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_202240, whole genome shotgun sequence.
ACCESSION   DATCRO010000946 DATCRO010000000
VERSION     DATCRO010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202240"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /locus_tag="VIN75_25890"
     CDS             complement(<1..2388)
                     /locus_tag="VIN75_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEY8977672.1"
                     /translation="MKQNYVKATTNICIQVAAGVLIAAGAASAAAADPALAPACDAPA
                     FATTLVAAADTTATAARALWLSQSLVRWPGAPAKGRYVLASSAKGALVAQVGQPVTGA
                     DDTLELAPTTDPLPAALAQRFKYVADGVVLRAGDRARVTRALRGQALVVREDEQGRVL
                     EATALQAAGALDDLYASAGDLADLGATRTKDALRLALWAPTAQAVHACVYPDADGASS
                     AVVALARDDATGAWRADIPGRSNGRYYSYLVDVFVPGTGLVRNRVTDPYAVSLNADSR
                     RTWLGTLDDAATQPRDWRRDAAPATVRRSTDMAIYELHLRDFSVGDASVPAAHRGKYL
                     AFTDANSAGMRHLRRLAQSGLTDVHLLPVFDIASVPERGCTTPTIAGGPADETQQAAV
                     VAGAASDCFNWGYDPFHYNAPEGSYSSDANDGPVRVREFRAMVQGLHHAGLRVGMDVV
                     YNHTSTSGQQPQSVLDRVVPGYYQRLAADGGIEHSTCCDNTATENLMMGKLLVDSVTQ
                     WAVQYHIDSFRFDLMGHQPRAVMEALQRRLKAATGREIPLIGEGWNFGEVADGARFVQ
                     ASQLSLGGTGIGTFSDRARDAVRGGASTDSSHLRADQGWINGEFYDPNGFGHATREDL
                     LAAADMIRVGLAGSLRDMPLATWRGQSTTFDKIPYGNQPAGYASQPGEAVNYVENHDN
                     QTLFDNDVLKLPLATSGADRARVQILGAAVVAFSQGVAYFHAGVDLLRSKSFDKNSFD
                     SGDWFNRIDWTGRDNGFGGGLPPRPDNGALWNIERPLLADASLKPTPADIAWTSDA"
     gene            2544..>4097
                     /locus_tag="VIN75_25895"
     CDS             2544..>4097
                     /locus_tag="VIN75_25895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8977673.1"
                     /translation="MTCRPSALPAALALAGTVLLGACASPARPGVDISRVQQVPRPAP
                     LAAGWEHGAFMEVFVRSYQDSNGDGVGDLRGLMQRLPYLQALGVRGLWLMPVNASSDH
                     DHGYAVTDYRAIEPAYGTMADFDELVREAHARGIGIVIDYVINHSSWKNPLFEQSASS
                     RTNPWRDWYVWEDVKPTGWNIFDIDPWNATPNGAYFSQFGKEMPDFNLRNPAVVQYHL
                     DSLRFWINHGVDGFRIDAVGHLFENGPQGWSLQPENYAFMARVRHELDIDHPHMMVCE
                     SPGDPAGFARQDACGASFAFGHSDALVRAGRGDEKAIHDVADYFLTAPDTMATMVSNH
                     DHFAGKRLWDAMAGNPARVRVAAAAYLLQPGTPYVYYGEELGMSAAATDDLDGKLRGP
                     MSWTPYGGFSSGKPYRPASTNLATHNAESEARDPDSMLAFYTAMLRLRNTHSSIATGR
                     YVAPRVDGRTMSFQRVDERERIVVVINYGDAPGSATLAGLPANARLQRLYPPTAGGDA
                     VASAAGGLSV"
BASE COUNT          586 a         1455 c         1454 g          602 t
ORIGIN      
        1 cgcgtcgctc gtccaggcga tgtcggcagg cgtcggcttg agcgaggcgt cggctagcag
       61 cggacgctcg atgttccaca gggcgccgtt gtcgggccgg ggcggcaggc cgccgccgaa
      121 gccgttgtcg cggcccgtcc agtcgatgcg gttgaaccag tcgcccgagt cgaagctgtt
      181 cttgtcgaag gacttcgagc gcagcaggtc gacgcccgca tggaagtacg cgacgccctg
      241 gctgaacgcg accaccgcgg cgcccaggat ctgcacgcgc gcccggtcgg cgccggacgt
      301 ggccagcggc agcttgagca cgtcgttgtc gaacagcgtc tggttgtcgt ggttctcgac
      361 gtagttgacc gcctcgccgg gctggctcgc gtagcccgcg ggctggttgc cgtacgggat
      421 cttgtcgaag gtcgtcgact ggccgcgcca ggtggccagc ggcatgtcgc gcagcgagcc
      481 cgcgaggccc acccggatca tgtcggcggc ggccagcagg tcctcgcgcg tggcgtggcc
      541 gaagccgttg gggtcataga actcgccgtt gatccagcct tggtcggcgc gcaggtgcga
      601 gctgtcggtc gacgcgccgc cgcgcaccgc gtcgcgggcg cgatcgctga aggtgccgat
      661 gcccgtgccg ccgagcgaca gctgcgacgc ctgcacgaag cgcgcgccgt cggccacctc
      721 gccgaagttc cagccctcgc cgatcagcgg gatctcgcgg ccggtggccg ccttcaggcg
      781 tcgctggagt gcctccatca cggcccgcgg ctggtggccc atcaggtcga agcggaacga
      841 gtcgatgtgg tactgcacgg cccactgcgt caccgagtcg acgagcagct tgcccatcat
      901 gaggttctcg gtggccgtgt tgtcgcagca cgtggagtgc tcgatgccgc cgtccgcggc
      961 cagccgctgg tagtagcccg ggacgacgcg atcgagcacc gactgcggct gctggccgct
     1021 cgtgctggtg tggttgtaga cgacgtccat gcccacgcgc aggccggcgt ggtgcaggcc
     1081 ttgcaccatc gcgcggaact cgcgcacgcg caccgggccg tcgttcgcgt cgctcgaata
     1141 gctgccttcg ggcgcgttgt agtggaacgg gtcgtagccc cagttgaagc agtcggaggc
     1201 cgcgccggcg acgacggccg cctgctgcgt ctcgtcggcc ggtccgccgg cgatcgtggg
     1261 cgtggtgcag ccgcgctcgg ggacgctggc gatgtcgaac accggcagca ggtgcacgtc
     1321 ggtcaggccg gactgcgcga ggcggcgcag gtggcgcatg ccggcgctgt tggcgtcggt
     1381 gaacgcgagg tacttgccgc ggtgtgccgc gggcacgctg gcgtcgccga cggagaaatc
     1441 gcgcaggtgc agttcgtaga tcgccatgtc ggtcgagcgc cggaccgtcg caggcgcggc
     1501 gtcgcggcgc cagtcgcgcg gctgcgtggc ggcgtcgtcc agcgtgccga gccaggtgcg
     1561 gcgcgaatcg gcattcaggc tgaccgcgta cggatcggtg acgcggttgc gcacgaggcc
     1621 cgtgccgggg acgaacacgt cgacgaggta gctgtagtag cggccgttcg accggccggg
     1681 gatgtcggcg cgccaggcgc cggtggcgtc gtcgcgcgcg agagcgacga cggcgctcga
     1741 ggccccgtcg gcgtccggat agacgcaggc gtgcacggct tgggcggtcg gcgcccacag
     1801 cgcgaggcgc agcgcgtcct tggtgcgggt cgcgcccagg tcggccagat cgccggccga
     1861 ggcgtagaga tcgtccagcg cgccggcggc ctgcagcgcg gtcgcctcca gcacgcggcc
     1921 ctgctcgtcc tcgcgcacga cgagggcctg cccgcgcagc gcgcgcgtga cgcgggcgcg
     1981 gtcgcccgcg cgcaggacca cgccgtcggc gacgtacttg aagcgttgcg ccagcgccgc
     2041 cggcagggga tcggtggtcg gcgcgagctc caacgtgtcg tcggcgccgg tcaccggctg
     2101 gccgacctgc gcgaccagcg cgcccttcgc agaggacgcc agcacatagc gccccttggc
     2161 cggcgcaccc ggccagcgta cgagcgactg cgacagccac agcgcgcgcg ccgccgtcgc
     2221 cgtcgtgtcg gccgccgcca ccagcgtcgt ggcgaaggcg ggggcgtcgc aggccggcgc
     2281 aagggccgga tcggcggccg ccgccgacgc ggctccggcc gcgatcagga cgcccgcggc
     2341 gacctggatg caaatgtttg ttgtggcctt cacgtagttt tgcttcacca ccgtgttgtc
     2401 tctgtattca caggaaggaa tgctacatgc tatttcaacg cgcctccaat aacgcgcatg
     2461 ggtttccctc attcggcctg ttcttgtgtt ggatttatga tgtactttca ctacatacca
     2521 tgacaacttc gagacacgcc gccatgactt gcagacccag tgccctgccc gccgccctcg
     2581 ccctggccgg caccgtcctc ctgggcgcct gcgccagccc ggcccgcccg ggcgtcgaca
     2641 tttcccgagt ccagcaggtg ccccgccccg cgccactggc ggcaggctgg gagcacggcg
     2701 cgttcatgga ggtgttcgtg cgcagctacc aggacagcaa cggcgacggc gtcggcgacc
     2761 tgcgcggcct catgcagcgc ctgccctacc tgcaggcgct cggcgtgcgc ggcctgtggc
     2821 tgatgccggt caacgccagc agcgaccacg accacggcta cgccgtgacc gactaccgcg
     2881 ccatcgagcc ggcctacggc acgatggccg acttcgacga actggtgcgc gaggcgcatg
     2941 cacgcggcat cggcatcgtg atcgactacg tcatcaacca cagctcgtgg aagaacccgc
     3001 tgttcgagca gtcggcctcg tccaggacca acccctggcg cgactggtac gtgtgggagg
     3061 acgtcaagcc caccggctgg aacatcttcg acatcgaccc gtggaacgcg acgcccaacg
     3121 gcgcgtactt cagccagttc ggcaaggaga tgcccgactt caacctgcgc aacccggcgg
     3181 tcgtgcagta ccacctggac agcctgcgct tctggatcaa ccacggcgtg gacggcttcc
     3241 gcatcgacgc cgtgggccac ctgttcgaga acggcccgca aggctggagc ctgcagcccg
     3301 agaactacgc gttcatggcc agggtgcgcc acgaactgga catcgaccac ccgcacatga
     3361 tggtgtgcga gtcgcccggc gacccggcgg gcttcgcgcg ccaggacgcc tgcggcgcgt
     3421 cgttcgcgtt cgggcacagc gatgcgctgg tcagggccgg gcgcggcgac gagaaggcga
     3481 tccacgacgt cgccgactac ttcctgaccg cgcccgacac catggccacg atggtctcca
     3541 accacgacca cttcgccggc aagcggctgt gggacgcaat ggccggcaac ccggcgcgcg
     3601 tgcgcgtcgc tgccgcggcc tacctgctgc agcccggcac gccctacgtc tactacggcg
     3661 aggagctggg catgtccgcc gccgccaccg acgacctcga cggcaagctg cgcggcccca
     3721 tgagctggac gccgtacggc ggcttcagca gcggcaagcc ctaccgtccg gcctcgacca
     3781 acctggccac gcacaacgcc gagtccgagg cgcgcgatcc cgactcgatg ctggcgttct
     3841 acacggcgat gctccggctg cgcaacacgc actcgtcgat cgccaccggc cgctacgtcg
     3901 ccccgcgcgt cgacggccgc acgatgagct tccagcgcgt ggacgagcgc gaacgcatcg
     3961 tcgtcgtcat caactacggc gatgcgcccg gcagcgccac gctcgcggga ctgccggcga
     4021 acgcgcgact gcagcgcctg tatccgccga ccgccggcgg ggacgccgtc gcctccgccg
     4081 cggggggcct gtccgtg
//
LOCUS       DATCRO010000947         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_146117, whole genome shotgun sequence.
ACCESSION   DATCRO010000947 DATCRO010000000
VERSION     DATCRO010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146117"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1596
                     /locus_tag="VIN75_25900"
     CDS             412..1596
                     /locus_tag="VIN75_25900"
                     /inference="COORDINATES: protein motif:HMM:NF015462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0008294 - calcium- and
                     calmodulin-responsive adenylate cyclase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthrax toxin-like adenylyl cyclase
                     domain-containing protein"
                     /protein_id="HEY8977674.1"
                     /translation="MRYNDTAFLRDTIGMPAAHEDAFLNVARSQRCVIITRATGPTCL
                     GPLEEGYDTKGYRIHGKSCDWGPMAGFVMRDPRLNKYGLEKAAFNAEKHDEALYHDHE
                     EQGWKASTTPLFISNARLAWLQSAGLLPGLRYDRDVQGYIGIANHGGVLFNYALVEER
                     GDLFSVCFDNRFAGCVWAQELGKVLKRPSHARIGKLEHMMAMTNPPGHGIGDRVAPHL
                     KAITGDYDLFAVWPYASAYNAAPGGDDHRPLGTVRGSNNNFDKSRITALEMDFTTNGQ
                     GTKLGNITNRIYMVCQLINSLVGRNVLWHSDESARPFLDDVDLPVAAFCPAGTHFGIE
                     TIHDFKAFIAACLQESIHVTLSDAWVTEPFGRKQNRLGYHYAGFVPADGVRVIVPAWY
                     NR"
     gene            complement(1789..2220)
                     /locus_tag="VIN75_25905"
     CDS             complement(1789..2220)
                     /locus_tag="VIN75_25905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977675.1"
                     /translation="MSAPPDAAPSGGQNHWRLLGYIAGLPRGRIVLWCYLAWYLVIAA
                     RYFDPAPAIWLNAAGISAVIGSALWLGVRQPGTGIGFWPTFRLYLTPFCVSSFSSLIK
                     GRGFVLVFPQDGAVLAQAAGACGAFVALVILVKAAAKASRR"
     gene            complement(2220..2924)
                     /locus_tag="VIN75_25910"
     CDS             complement(2220..2924)
                     /locus_tag="VIN75_25910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977676.1"
                     /translation="MTTRPLPPDHELRTLTAADLPALHQLHEAVLGALPDPAMFRLFG
                     GAQKFLSDHLDARGRSLGIFADGRLVAYGSLTRPAAGDLDNYAVDAGWAPDRAGRVAL
                     LSAAMVHPSHRIQGLHSALIARRIELARELGAPELLVRAAPANAVSRRTLLSHGFAVA
                     WLGVQRDGSLRHVLWRPIGGPAWTAPSPGEPEIAWVAADDLPGQQRLLAEGWLGVRMR
                     AGDAALGFARPAPPAA"
     gene            complement(2921..3952)
                     /locus_tag="VIN75_25915"
     CDS             complement(2921..3952)
                     /locus_tag="VIN75_25915"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8977677.1"
                     /translation="MKRRDVLALALPLAATRANGAGALRATPAPWRIHAVTYRGRTDV
                     ERGFADYFAARGLPVEITWRDIALDPGRLPALVDEIRRERPHLVHTWGTGVTLGIAGR
                     WDAPDDGRHIRDIPIVFSLVASPVGAGIVPDVHAPGRPVTGVSHVASLAVQLQTMASY
                     RPFRAVGMLYSPNEPNSAAVLEELQALGRQRGIAVLATPFRVNAGGQPVAGGAEDLVR
                     QLRAGGAEWLYMPPDSFLSTQARDIVVPAALAQGVPAFASTEQLMSAGALLGLVSRYY
                     NVGQFAAYKAERILSGQARADRIPVETLTRFSLQINLDVARRLGVLPPLEMFNYAELI
                     GDAGKGNPS"
     gene            complement(3949..>4578)
                     /locus_tag="VIN75_25920"
     CDS             complement(3949..>4578)
                     /locus_tag="VIN75_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977678.1"
                     /translation="GQATAAPAAPAPRDYARVFAQGRFLWVTALAAVPAKLILAGSVF
                     YLVPLYVAAAGASPSVAGRAMMLYAVALVLVLPRATHWVERGVPLARMVGAGLCVSAL
                     GGFALVAGDGVLPVYLVTALLGLGQGLSIAAQSSLLSQVCANEIAAHGSGPAFGAYRL
                     LERLGNASGPIVAGAFALVAGPASAFVAMAGLVLACGLSFLLLARRVAR"
BASE COUNT          733 a         1629 c         1662 g          554 t
ORIGIN      
        1 aggtcgatcg tcgccgccgt catgccgaac aggcccgacg cgtgcgggat gggcgtcatc
       61 ggcagggccg ccaccggcag cagcgccacg cagcccgcca gcagcagcag gcacagcccg
      121 aggaccacgc gccgttgggc gagggtggcg ggctgggcgg cgaaatgcat ggcgaaggcc
      181 agtgtagggg acgcggacat ggcgtcgccg gcggcgggcg ggcgccaagc gagggagttg
      241 gcatccgttt gccatcggtg gaccggacgg cgccaccttc gaactagggg ccgcggcgcg
      301 ccgttcgtga ggtttcccgc caagtcggcg cgccatcggt gtccacggca ttgcccagcc
      361 gggtcgcagc tcctagcatc ggcgcggcat caggacaaag aggactgcga catgcgctac
      421 aacgacacgg cttttcttcg cgacaccatc ggcatgcccg ccgcccacga ggacgcgttc
      481 ctcaacgtgg cgcgctcgca gcgctgcgtg atcatcacgc gcgccaccgg gccgacctgc
      541 ctcgggccgc tggaggaggg ctacgacacc aagggctacc gcatccacgg caagtcctgc
      601 gactggggcc ccatggcggg cttcgtcatg cgcgatccgc gcctgaacaa gtacggcctc
      661 gagaaggccg ccttcaacgc cgagaagcac gacgaggcgc tgtatcacga ccacgaggag
      721 cagggctgga aggcgtcgac cacgccgctg ttcatctcga acgcgcgcct ggcctggctg
      781 cagtcggcgg gcctgctgcc gggcctgcgc tacgaccgcg acgtgcaggg ctacatcggc
      841 atcgccaacc atggcggcgt gctgttcaac tacgcgctcg tcgaggagcg cggcgacctg
      901 ttcagcgtgt gcttcgacaa ccgtttcgcc ggctgcgtct gggcgcagga actcggcaag
      961 gtgctcaagc gcccgagcca tgcgcgcatc ggcaagctcg aacacatgat ggcgatgacc
     1021 aacccgcccg gccacggcat cggcgaccgc gtggcgccgc acctgaaggc catcacgggc
     1081 gactacgacc tgttcgcggt ctggccctac gccagcgcct acaacgcggc ccccggcggc
     1141 gacgaccacc gccccctcgg taccgtgcgc ggctcgaaca acaacttcga caagagccgc
     1201 atcaccgccc tggagatgga cttcaccacc aacggccagg gcaccaagct gggcaacatc
     1261 accaaccgca tctacatggt gtgccagctg atcaactcgc tggtcgggcg caacgtgctg
     1321 tggcacagcg acgagtccgc gcgtcccttc ctcgacgacg tcgacctgcc cgtcgccgcc
     1381 ttctgcccgg ccggcacgca cttcggcatc gagaccatcc acgacttcaa ggccttcatc
     1441 gccgcctgcc tgcaggaaag catccacgtc acgctgtccg acgcctgggt cacggagccg
     1501 ttcggcagga agcagaaccg gctcggatat cactacgccg gcttcgtgcc cgccgatggc
     1561 gtgcgcgtga tcgtgccggc ctggtacaac cgttgaagcc ggccagtgtc gtggttggaa
     1621 attcgttgaa caggtctgga gcgaagaagt gacggtgccg atttccgtgc agaacggttg
     1681 cagaccaggc gcgcacgcga cacgatgccg ggtggcctcg cgagcgggcg caacgacgga
     1741 tgcggccgtc ccgggcggaa ttcttcaacg aatttccaac tcgcgacact agcggcgact
     1801 ggccttcgca gcggccttca ccaggatgac caacgccacg aacgcgccgc aggcgcccgc
     1861 ggcctgcgcc agcacggcgc cgtcctgcgg gaacacgagc acgaagccgc ggcccttgat
     1921 cagcgacgag aagctcgaga cgcagaacgg cgtgaggtac agccggaacg tcggccagaa
     1981 tccgatgccc gtgccgggct ggcgcacgcc cagccacaag gcgctgccga tgacggcgct
     2041 gatgccggcc gcgttgagcc agatcgcggg cgccggatcg aaatagcggg ccgcgatgac
     2101 caggtaccag gcgaggtagc accagagcac gatccgcccg cgcggcaggc cggcgatgta
     2161 gccgaggagc ctccagtggt tctgtccgcc cgaaggggcg gcatcgggag gtgcggacat
     2221 caggccgccg gcggcgcggg gcgcgcgaat ccgagcgcgg cgtcgcctgc gcgcatgcgc
     2281 acgccgagcc agccctcggc cagcaggcgc tgctgcccgg gcaggtcgtc cgcggccacc
     2341 caggcgatct ccggttcgcc tggtgacggc gcggtccacg ccggtccgcc gatcggtcgc
     2401 cagagcacgt gccgcagcga gccatcgcgc tgcaccccca gccaggcgac ggcaaagccg
     2461 tgcgacagca gcgtgcggcg cgacaccgcg ttggccggcg ccgcgcgcac cagcagttcc
     2521 ggggcgccga gctcgcgcgc gagctcgatg cggcgcgcga tcagcgcgct gtgcaggccc
     2581 tggatgcggt gcgagggatg caccatcgcc gccgacagca gcgccacgcg gcccgcccga
     2641 tcgggcgccc agccggcgtc gacggcgtag ttgtcgaggt cgccggcggc gggtcgagtc
     2701 agcgagccgt aggccacgag ccggccgtcc gcgaagatgc ccaggctgcg gccgcgggcg
     2761 tcgaggtggt cggacaggaa cttctgcgcg ccgccgaaca ggcggaacat ggcggggtcg
     2821 ggcagcgcgc ccagcacggc ttcgtgcagc tggtgcagtg ccggcaggtc ggcggccgtg
     2881 agcgtgcgca gctcgtggtc cggcggcagc gggcgtgtgg tcatgaaggg ttccctttgc
     2941 cggcgtcgcc gatcagctcg gcgtaattga acatctcgag cggcggcagg acgcccaggc
     3001 ggcgtgcgac gtccaggttg atctgcagcg agaagcgcgt caaggtctcg accgggatgc
     3061 gatcggcccg ggcctggccg gacaggatgc gttccgcctt gtaggccgcg aactggccca
     3121 cgttgtagta gcggctgacc aggcccagga gcgcgcccgc cgacatcagc tgctcggtcg
     3181 acgcgaaggc cggcacgccc tgcgcgagcg ccgccggcac gacgatgtcg cgcgcctgcg
     3241 tcgacaggaa cgagtcgggc ggcatgtaga gccactcggc accgccggcg cgcagctgcc
     3301 ggacgagatc ctccgcgccg ccggcgaccg gttggccgcc ggcgttgacg cggaacgggg
     3361 tggccagcac ggcgatgccg cgctggcgtc cgagcgcctg cagttcctcg agcacggccg
     3421 ccgagttcgg ttcgttgggc gagtacagca tgcccacggc gcggaatggc cggtagctgg
     3481 ccatcgtctg cagttgcacg gcgaggctgg cgacgtggct gacgccggtc accggccgcc
     3541 cgggggcgtg cacgtcgggc acgatgcccg cgcccaccgg ggacgcgacg aggctgaaga
     3601 cgatggggat gtcgcggatg tggcgaccgt cgtccggcgc gtcccagcgg ccggcgatgc
     3661 ccagcgtgac gccggtgccc caggtgtgga cgaggtgagg gcgctcgcgc cggatttcgt
     3721 cgaccagcgc gggcaggcgc cccgggtcga gcgcgatgtc gcgccaggtg atctcgaccg
     3781 gcaggccgcg cgcggcaaag tagtccgcga acccgcgctc gacgtcggtg cggccgcggt
     3841 aggtcacggc atggatgcgc cacggcgccg gcgtggcgcg gagtgcgccg gcgccgttgg
     3901 cgcgcgtcgc cgcaagcggc agcgccaggg cgaggacgtc gcgccgcttc atcgcgcgac
     3961 ccttcgggcc agcagcagga acgacaggcc gcaggccagc acgaggccgg ccatcgccac
     4021 gaatgccgac gcgggcccgg cgacgagcgc gaacgcgccc gcgacgatcg gccccgaggc
     4081 gttgcccagg cgctcgagca gccggtaggc gccgaacgcc ggcccgctgc cgtgcgcggc
     4141 gatttcgttg gcgcagacct gggacagcag gctgctctgc gcggcgatcg acaggccctg
     4201 gcccaggccc agcagcgccg tcacgaggta gaccggcagc acgccgtcgc cggccaccag
     4261 tgcgaagccc ccgagcgccg acacgcacag gcccgcgccc accatgcgcg cgagcggcac
     4321 gccgcgctcg acccagtgcg tggcacgcgg cagcacgagc acgagcgcca ccgcgtacag
     4381 catcatggcg cgcccggcca ccgacggcga cgcgcccgcc gcggccacgt acagcggcac
     4441 gaggtagaac accgagcccg cgaggatcag cttggccggc accgccgcga gcgccgtcac
     4501 ccacaggaag cggccctgcg cgaacacgcg ggcgtagtcg cgcggtgccg gagcggccgg
     4561 cgcggccgtc gcttggcc
//
LOCUS       DATCRO010000948         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_731078, whole genome shotgun sequence.
ACCESSION   DATCRO010000948 DATCRO010000000
VERSION     DATCRO010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731078"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIN75_25925"
     CDS             complement(<1..357)
                     /locus_tag="VIN75_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEY8977679.1"
                     /translation="MSDALEHDPVPAGVTRRLGLRRHVPVILQTEASECGLAALAMVA
                     VALGLDTDLPALRLRFGVSRKGANFEGLVRVAAALGLDSRPLKLDLHNLRELQLPCIL
                     HWDMNHFVVLVSVSARR"
     gene            complement(464..1723)
                     /locus_tag="VIN75_25930"
     CDS             complement(464..1723)
                     /locus_tag="VIN75_25930"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8977680.1"
                     /translation="MPELFRRQAVDHQRQKFHGAIVLTRSPWRTSIAAFFVLLVLALL
                     VFAGTQGFARKETVGGVLAPAGGVLRLVAPQAGVIVATPVPQGSRVAAGQPVVRLSAE
                     QSSAIGPTQEAVARSLALRQGSLADELRQQGEQVRQQALSLDARIAAVQSGLAQQERE
                     IALQRERVQLVREVAARYPDLVRSGAVSPVEAAEKQTELIDQQSRLSELERAHGASQA
                     ELATLRADRANLPLQAGRESSQMRREVQALAQAQAENESHRGMQVLAPEAGDVATLLA
                     APGQSVAAGQTLATLLPAGAVLEAELWVTTRAAGFVHPGTPVWLRVDAFPYARFGQLP
                     AHVREVSRSAVNAGDLGESAGAAAASGQDVFRVIVTLDAVPADAAPAWRASLKAGMRV
                     QASLVAERRTLLQWALEPLAALRVAAR"
     gene            1931..2617
                     /locus_tag="VIN75_25935"
     CDS             1931..2617
                     /locus_tag="VIN75_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8977681.1"
                     /translation="MKLLIGNKNYSSWSMRPWVLLTEFDIPFEEVHVPFESFAPDSRF
                     KAAVAERSPAGKVPVLVEDDGFAVWDSLAIAEYVAEKFPAKALWPHDARRRARARSVC
                     AEMHSGFAAMRDVFGMNIEASLPQIGARVLAANAAARADLARMIELWQEALAASGGPF
                     LFGDFSIADAYFAPVVMRMRTYALPVPADVAAYVERAVAAKGPAAWIRGALAEKQFLQ
                     HEEAYRTSRD"
     gene            2697..>3174
                     /locus_tag="VIN75_25940"
     CDS             2697..>3174
                     /locus_tag="VIN75_25940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977682.1"
                     /translation="MSVPRIPALAPVALAVVALLATGQSSAQSTTEPAASAPSAAQAA
                     ASIAQPAPRAAAPAAVAAPAASAATSQPTASTEAPVVPSAAASAALAPVALAVVALLA
                     TGRAFSQSAVEAEPPVSAPSAAAAATAAPEAPAQAPAVAHVPAASQAAAHGPAASQA"
BASE COUNT          406 a         1234 c         1131 g          403 t
ORIGIN      
        1 gcgccgcgcc gacaccgaca ccagcaccac gaagtggttc atgtcccagt gcaggatgca
       61 gggcagctgc agctcgcgca ggttgtgcag gtccagcttc agcggacggc tgtccaggcc
      121 gagcgcggcg gccacgcgca ccaggccctc aaagttggcg cccttgcgcg agacgccgaa
      181 gcgcaggcgc agcgccggca ggtcggtgtc caggccaaga gcgaccgcca ccatcgccag
      241 cgccgccagc ccgcactcgc tggcctcggt ctgcaggatc accggcacgt gccggcgcag
      301 ccccaggcgg cgcgtgacgc cagcgggcac ggggtcgtgt tccaaggcgt cgctcattgg
      361 agcccctccc gcgccgagta cggcgctccg tcccccgagg ggactgcggg cctccttggg
      421 gcggcccggc gcatggcccg caaacaggtt ggcacaggat gtctcatctg gcggccaccc
      481 gcagcgcggc cagcggctcc agcgcccatt gcagcagcgt gcggcgctcg gccaccaggc
      541 tggcctgcac gcgcatgccg gccttcagcg acgcgcgcca ggcgggcgcg gcgtcggcgg
      601 ggaccgcatc cagcgtcacg atcacgcgaa agacgtcctg gcccgacgcc gccgcggcgc
      661 cggcggactc gcccagatcg ccggcattga cggcgctgcg cgaaacctcc cgcacatggg
      721 ccggcagctg gccgaagcgc gcgtacggga acgcgtcgac gcgcagccac acgggcgtgc
      781 ccggatgcac gaagcctgcc gcgcgcgtgg tcacccacag ctcggcctcc agcaccgcgc
      841 cggcgggcag gagggtggcc agcgtctggc cggcggcgac ggactgcccg ggcgcggcca
      901 gcagcgtggc gacgtcgccg gcctcggggg cgagcacctg catgccgcgg tgcgactcgt
      961 tctcggcctg cgcctgcgcg agcgcctgca cctcgcgccg catctgcgag ctctcgcggc
     1021 cggcctgcag cggcaggttg gcgcggtcgg cgcgcagcgt ggcgagctcg gcctgcgaag
     1081 cgccgtgcgc gcgctcgagc tccgacaacc gcgactgctg gtcgatcagc tcggtctgct
     1141 tctcggccgc ctccacgggc gagaccgcgc cgctgcgcac gagatccgga tagcgcgccg
     1201 ccacctcgcg caccagttgc acccgctcgc gctgcagcgc gatctcgcgc tcctgctgcg
     1261 cgaggcccga ctgcaccgcc gcgatgcgcg catccagcga gagcgcctgc tggcgcacct
     1321 gctcgccctg ctggcgcaat tcgtcggcga gactgccctg gcgcagcgcc agcgagcgcg
     1381 cgacggcctc ctgcgtgggc ccgatcgcgc tcgattgctc ggccgacagc cgcaccaccg
     1441 gctgccccgc cgccacgcgg ctgccctgcg ggacgggtgt cgccacgatc acgccggcct
     1501 gcggcgccac cagccgcagc acgccgccgg ccggcgccag cacgccgccc acggtctcct
     1561 tgcgggcgaa gccctgcgtc cccgcgaaca ccagcagcgc cagcaccagc agcacgaaga
     1621 aggccgcgat cgacgtccgc cacggactgc gcgtcagcac gatcgcgcca tggaacttct
     1681 ggcgctgatg gtcgacggcc tggcggcgga acagttcggg catgggattg acggggcggc
     1741 ttcgcgcgac cgcgaagggt agcctgaaaa cgcggcacgg ccatcagcga ttgcccgtcg
     1801 gtcgcgccgg gcgcggattc cgcggtcgca tggccggcat gcacattcgt catcggggat
     1861 ggcgcgcgcc ggccgctaca gtcgcgtttc ccacacgaca cgccgcgttc gaacgcgcac
     1921 ggagcctcgc atgaagctgt tgatcggcaa caagaactac tcgtcctggt cgatgcgccc
     1981 ctgggtgctg ctcaccgaat tcgacatccc gttcgaggag gtgcacgtcc cgttcgagag
     2041 cttcgcgccg gattcgcgct tcaaggccgc ggtggccgag cgctcgcccg cgggcaaggt
     2101 gcccgtcctg gtcgaggacg acggctttgc cgtgtgggac tcgctggcga tcgccgagta
     2161 cgtcgccgag aagttccccg ccaaggcgct gtggccgcac gacgcccgcc gccgcgcacg
     2221 tgcccgcagc gtctgcgccg agatgcacag cggctttgcc gcgatgcgcg acgtcttcgg
     2281 catgaacatc gaggcctcgt tgccgcagat cggcgcccgc gtgctggccg cgaacgccgc
     2341 ggcgcgcgcc gacctcgccc gcatgatcga gctgtggcag gaggcgctcg ccgcgtcggg
     2401 cggcccgttc ctgttcggcg acttctcgat cgccgacgcc tacttcgcgc cggtggtcat
     2461 gcgcatgcgc acctacgcgc tgccggtgcc ggccgacgtg gccgcgtacg tcgagcgcgc
     2521 cgtcgccgcc aagggcccgg ccgcgtggat ccgcggggcg ctggccgaga agcagttcct
     2581 gcagcacgag gaggcgtacc gcacgtcgcg cgactgacgc gaacgcgcga cgcgggcccg
     2641 ttccgcctgc cttttcgcgg gagccgcgga cacgtccttc ggtatattgg ccagacatgt
     2701 ccgttccccg aattccagcg ctggcgcctg tcgcgctcgc cgtcgtcgcg ttgctggcca
     2761 cgggccagtc gtccgcccag tccaccaccg agcccgcggc atcggcgccg tcggccgcgc
     2821 aggccgccgc atcgatcgcg cagcctgcac cccgcgctgc ggcgccggcc gcggtcgcgg
     2881 cgcccgcggc ctccgcggcg acatcccagc cgacggcgtc gaccgaggcg ccggtcgtgc
     2941 catcagccgc cgcgtcggcc gcgctggcgc cggtcgcgct ggccgtcgtc gcgctgctgg
     3001 ccaccggccg cgcgttctcc cagtccgccg tcgaagccga gccgccggtg tcggcgccgt
     3061 cggccgccgc cgcggcgacg gccgcgccgg aagcgccggc acaggcgccc gccgtggcgc
     3121 atgtgccggc cgcgtcgcag gccgccgcgc atgggcctgc cgcgtcgcag gcgg
//
LOCUS       DATCRO010000949         6499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1090605, whole genome shotgun sequence.
ACCESSION   DATCRO010000949 DATCRO010000000
VERSION     DATCRO010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090605"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VIN75_25945"
     CDS             complement(<1..1795)
                     /locus_tag="VIN75_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY8977683.1"
                     /translation="MSSATLDSRQIALLGNPNCGKTALFNLLTGSRQKVANYAGVTVE
                     RKEGLLTLPSGKQVRVLDLPGAYSLNATSADETVTRDVVQGKRAGEAPPDLLVCVTDA
                     TNLRLTLRLVIEARRLKRPMLVALNLADVARKRGIHIDVRKLEAELGVPVVETVAVKT
                     GGAESLTAAVDAWLARSHEPAQAPASGPGVHFDAEPPHGHVGQAAIDAVMRDHHEVHR
                     LLEATLTEPAASTHVDDRLDRVLLHPVAGMAILTFVLFLMFQAVFSWAKWPMEQIQTL
                     MDGTGQLVARHMDDGMLRSLLVDGVIAGAGSVLVFLPQILILFLFILALEDSGYLPRA
                     AFLLDRVMASVGLTGRSFIPLLSSFACAIPGIMATRTIADQRDRLATILIAPLMTCSA
                     RLPVYALLIGAFIPARTLSFGVNLQGLVLFVLYMAGIVSAMAVAWVLQRLGRRTGPAH
                     HTLMMELPAYRWPNLRNLGIGLLERAKIFTTRVGTIILALMILLWFLSSFPAPPAGAT
                     GPAITYSLAGRLGRLLEHVFAPIGFNWQISIALVPGLAAREVAVGALGTVYAMSASGD
                     DLANQLGPVIAGGWSLATACSLIAWYVFAPQC"
     gene            complement(1792..2175)
                     /locus_tag="VIN75_25950"
     CDS             complement(1792..2175)
                     /locus_tag="VIN75_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY8977684.1"
                     /translation="MPGQVLARLAEPTSPHAVDDARDRGTVAPAPATVPLTRLPRNAT
                     GTVAGVRVEALGPDGTLDPDVERMALRLIEIGFVQGASLRVIAFGQPGDDPIGVRVGG
                     RGGVSTFALRRAEAACVQVWPDRQS"
     gene            2478..3041
                     /gene="ahpC"
                     /locus_tag="VIN75_25955"
     CDS             2478..3041
                     /gene="ahpC"
                     /locus_tag="VIN75_25955"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEY8977685.1"
                     /translation="MSLINTQVQPFKTEAFHNGKFITVSDETLKGKWSVLIFMPAAFT
                     FNCPTEVEDAADNYAEFQRLGAEVYIVTTDTHFSHKVWHETSPAVGKARFPLVGDPTH
                     ALTNAFGVHIPEEGLALRGTFIINPEGTIKTLEIHSNEIARDVKETLRKLKAAQYTAA
                     HPGEVCPAKWNEGAKTIAPSLDLVGKI"
     gene            3166..4722
                     /gene="ahpF"
                     /locus_tag="VIN75_25960"
     CDS             3166..4722
                     /gene="ahpF"
                     /locus_tag="VIN75_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEY8977686.1"
                     /translation="MLDDNLKAQLKAYLERVKLPFTITASLDEGPSSQEMHEFLRDIV
                     GLTDKITLRTDGTDARRPSFSLARDGESERIRFAAIPMGHEFTSLVLALLQVGGHPPK
                     IEQELIEQIKGLDGDLRFETYMSLTCHNCPDVVQALNLLAVLNPRIQSVVIDGGLFRD
                     EVESRQVLAVPMVYLNGQHFGQGRMEVDEIVKKLDSGSAARDAAKLGGKDAFDVLIVG
                     GGPAGAAAAVYAARKGIRTGLLAERMGGQTMDTMSIENFISVLETDGPKFAAALENHA
                     KRYDVDIMNLQRATKLEPAGADGLIGVTLENGATLRSKTVIVSTGARWREVNVPGEKE
                     YRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVGHVTLLEFGEALRADA
                     VLVDKLKSLANVDVHVQAQTTEITGADGKVNGLTYTDRRNGEAKHVELEGVFVQIGLV
                     PNSEWLKGTMELTRHGEIVVDARGQTSVPGVFAAGDVTTVPFKQIIIATGDGAKAALG
                     AFDHLIRQGV"
     gene            complement(4924..6243)
                     /gene="xylA"
                     /locus_tag="VIN75_25965"
     CDS             complement(4924..6243)
                     /gene="xylA"
                     /locus_tag="VIN75_25965"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8977687.1"
                     /translation="MSDYFAGIDKIRYAGPQSTDPLSFKWYDKDRIVLGRRMEDQLRF
                     AVCYWHSFCWNGFDPFGYDGTFDRPWHRVADPMEAAKAKLDVAFEFFAKLGAPFYCFH
                     DRDVAPEGSTPRESVDNFRRIVDLMGEKQAATGVKLLWGTANLFSHRRFMSGAATNPN
                     PEIYALAALQVKEAMDATRRLGGANYVLWGGREGYETLLNTDLARESRQYGRFLQMAV
                     EHKHKLGLPGYVLIEPKPREPTKHQYDYDVATVHGFLRQYGLENEVKVNIEANHATLS
                     GHSFEHEIATAVALGLFGSLDMNRGDMQCGWDTDQFPNNLPETALALYHVLRGGGLTH
                     GGLNFDAKVRRQSIDPADLFHGHVGAMDLCARALLAAERMVTDGRLARDLDARYAGWD
                     GDVGRKLLSPGTSLDDAASHALQRNVDERPVSGRQEAWENLVNEFVA"
     gene            complement(6269..>6499)
                     /locus_tag="VIN75_25970"
     CDS             complement(6269..>6499)
                     /locus_tag="VIN75_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977688.1"
                     /translation="VHRGQPPAVVGAEAGGAMGAGRLAWLALGRDEREVCRPPAPRAL
                     FQPRAARRPALQARLERFRALYRHTRELMPRH"
BASE COUNT         1072 a         2188 c         2306 g          933 t
ORIGIN      
        1 ggcattgcgg cgcgaagaca taccacgcga tgagtgaaca tgcggtggcg agactccagc
       61 cgcccgcgat gaccggtccg agctggttgg cgaggtcatc acccgaggcc gacatcgcgt
      121 acaccgttcc gagagcgccg acggcgacct cgcgcgcggc gagtccgggc acgagcgcga
      181 tcgagatctg ccagttgaaa ccgatcggcg cgaagacgtg ctcgagcagg cggcccaggc
      241 ggccggcgag gctgtaggtg atcgcgggcc ccgtggcgcc ggccgggggc gcggggaagc
      301 tcgagaggaa ccacagcagg atcatcagcg cgaggatgat ggtgccgacg cgggtggtga
      361 agatcttcgc gcgctcgagc aggccgatgc cgaggttgcg caggttgggc cagcggtagg
      421 ccggcagctc catcatcagc gtgtggtggg cggggcccgt gcggcggccc aggcgctgca
      481 gcacccaggc gacggccatc gcgctgacga tgccggccat gtacagcacg aacagcacca
      541 ggccctgcag gttgacgccg aacgacagcg tgcgcgccgg gatgaacgcg ccgatcagca
      601 gcgcgtacac cggcagccgc gccgagcagg tcatcagggg cgcgatgagg atggtggcga
      661 ggcggtcgcg ctggtcggcg atggtccggg tggccatgat gccggggatc gcgcacgcga
      721 agctcgagag cagcgggatg aagctgcggc cggtcaggcc cacgctggcc atcacgcgat
      781 cgagcaggaa cgccgcgcgc ggcaggtagc ccgagtcctc gagcgcgagg atgaacagga
      841 acaggatcag gatctgcggc aggaacacga gcacgctgcc ggcgccggcg atcacgccgt
      901 ccaccagcag gctgcgcagc atgccgtcgt ccatgtggcg cgccaccagc tggccggtgc
      961 cgtccatcag cgtctggatc tgctccatcg gccacttggc ccagctgaac acggcctgga
     1021 acatcaggaa cagcacgaac gtgaggatgg ccatgcccgc caccggatgc agcagcacgc
     1081 ggtcgagacg gtcgtcgacg tgcgtgctgg ccgcgggctc ggtgagcgtg gcctcgagca
     1141 ggcggtgcac ctcgtggtgg tcgcgcatga cggcgtcgat cgcggcctgg cccacgtggc
     1201 cgtgcggcgg ctcggcgtcg aagtgcacgc cggggccgct cgcgggcgcc tgcgcgggct
     1261 cgtgcgagcg cgcgagccag gcgtcgacgg ccgcggtcag cgattccgcg ccgccggtct
     1321 tcacggccac cgtctcgacc accggcacgc ccagctcggc ctcgagcttg cggacgtcga
     1381 tgtggatgcc gcgcttgcgg gcgacgtcgg ccaggttcag cgcgaccagc atgggccgct
     1441 tcaggcgccg cgcctcgatg accaggcgca gcgtcaggcg caggttggtg gcgtcggtga
     1501 cgcacaccag caggtcgggc ggcgcctcgc ccgcgcgctt gccctggacg acgtcgcgcg
     1561 tgacggtctc gtcggcgctg gtcgcgttca ggctgtaggc gcccggcagg tccagcacgc
     1621 gcacctgctt gccggacggc agcgtcagca ggccttcctt gcgttcgacg gtgacgccgg
     1681 cgtagttggc caccttctgg cggctgccgg tgagcaggtt gaacagcgcg gtcttgccgc
     1741 agttcgggtt gcccagcagc gcgatctggc ggctgtccag agtggcggag ctcatgattg
     1801 cctgtccggc caaacctgca cgcaggccgc ttccgcgcgc cgcagcgcga acgtgctgac
     1861 gccgccacgg ccgcccaccc gcacgccgat cgggtcgtcg cccggctggc cgaaggcgat
     1921 cacgcgcagg ctcgcgcctt gcacgaagcc gatctcgatc aggcgcaacg ccatgcgctc
     1981 cacgtccggg tcgagcgtgc cgtcgggccc cagcgcctcg acgcgcacgc cggccacggt
     2041 gccggtggcg ttgcgcggca gccgggtcag cggcacggtg gcgggcgccg gcgcgaccgt
     2101 gccgcggtcg cgcgcgtcat cgacggcgtg gggcgacgtc ggttcggcga ggcgggcgag
     2161 cacttgtcca ggcatggcgt ttcaaaggag aggcgctgga gttctaaaga agaaccattc
     2221 tcattcattg taggcgcacc cgccacccct gtcatctgac ggccatcaag tccacatcgt
     2281 tcgcctgcga ctatcggccc gatagtttct atcaaagacc aggaaatcat agatttgcca
     2341 tagtgcctgc ccgggatgga gccgtcgtga gcgaggcggc cctatcgcaa cgatagctat
     2401 tgaaaatcac gaagtgatag ccaatagctt atgattcagt cattccgaat caccgccaac
     2461 cccaacgagg atgcaagatg tccctgatca acacccaagt ccaacccttc aagaccgaag
     2521 ccttccacaa cgggaagttc atcaccgtca gcgacgagac cctcaagggc aagtggtccg
     2581 tgctgatctt catgccggcc gccttcacct tcaactgccc gaccgaagtg gaagacgccg
     2641 ccgacaacta cgccgagttc cagaggctgg gagccgaggt ctacatcgtc acgaccgaca
     2701 cgcacttctc gcacaaggtg tggcacgaga cctcgccggc cgtcggcaag gccaggttcc
     2761 cgctggtcgg cgacccgacg cacgcgctga ccaacgcctt cggcgtgcac atcccggaag
     2821 aaggcctggc gctgcgcggc accttcatca tcaacccgga aggcaccatc aagacgctgg
     2881 agatccacag caacgagatc gcccgcgacg tcaaggaaac cctgcgcaag ctcaaggccg
     2941 cccagtacac cgccgcgcat ccgggcgaag tctgcccggc caagtggaac gaaggcgcga
     3001 agaccatcgc tccgtcgctc gacctcgtcg gaaagatctg aagtcgctgt cttgcgctcg
     3061 ccggcgcccg ggaggggacc gcgcgagcga cgcgccgggc gcggttcgaa ggccgccccc
     3121 gccgcttgtc cgatcatctg aatggttaga agaaggagtc cccgcatgct cgacgacaac
     3181 ctgaaggcac agctcaaggc ctacctcgag cgcgtgaagc tgcctttcac catcacggcc
     3241 tcgctggacg aaggcccgtc ctcgcaggag atgcacgagt tcctgcgcga catcgtcggc
     3301 ctgaccgaca agatcaccct ccggaccgac ggcacggatg cgcgccggcc gtcgttctcg
     3361 ctggcccgcg atggcgagtc cgagcgcatc cgcttcgcgg cgatcccgat gggccacgag
     3421 ttcacctcgc tggtgctggc gctgctgcag gtcggcggcc atccgcccaa gatcgagcag
     3481 gagctgatcg agcagatcaa gggcctcgac ggcgacttgc gcttcgagac ctacatgagc
     3541 ctcacgtgcc acaactgccc ggacgtcgtc caggcgctga acctgctcgc cgtgctgaac
     3601 ccgcgcatcc agagcgtggt catcgacggc ggcctgtttc gcgacgaggt cgagagccgc
     3661 caggtgctcg cggtgccgat ggtctacctg aacggccagc acttcggcca ggggcggatg
     3721 gaggtggacg agatcgtcaa gaagctcgac tccggctcgg cggcgcgcga cgccgccaag
     3781 ctgggcggca aggacgcctt cgacgtgctg atcgtcggcg gcggccccgc gggcgcggcc
     3841 gcggccgtgt acgcggcgcg caagggaatc cgcaccggcc tgctggccga gcgcatgggc
     3901 ggccagacga tggacaccat gagcatcgag aacttcatct cggtgctcga gacggacggc
     3961 ccgaagttcg ccgcggcgct ggagaaccac gccaagcgct acgacgtcga catcatgaac
     4021 ctgcagcgcg cgaccaagct cgagcccgcg ggcgcggacg gcctgatcgg cgtgacgctg
     4081 gagaacggcg caacgctcag gagcaagacc gtcatcgtct ccaccggcgc ccgctggcgc
     4141 gaagtgaacg tgccgggcga gaaggagtac cgcaacaagg gcgtggcgta ctgcccgcac
     4201 tgcgacggcc cgctgttcaa gggcaagcgc gtggccgtga tcggcggcgg caactcgggc
     4261 gtcgaggcgg cgatcgacct ggccggcatc gtgggccacg tcacgctgct ggagttcggc
     4321 gaggcgctgc gcgccgacgc ggtgctggtc gacaagctca agagcctggc caacgtcgac
     4381 gtccacgtgc aggcgcagac caccgagatc accggcgccg acggcaaggt caatggcctg
     4441 acgtacaccg accgccgcaa cggcgaggcc aagcacgtcg agctcgaagg cgtgttcgtg
     4501 cagatcggcc tggtgcccaa ctccgagtgg ctcaagggca cgatggagct caccaggcac
     4561 ggcgagatcg tggtcgacgc gcgcggccag acctcggtgc ccggcgtgtt tgccgccggc
     4621 gacgtcacga cggtgccgtt caagcagatc atcatcgcca ccggcgacgg cgccaaggcc
     4681 gccctgggcg cgttcgacca cctgatccgg cagggcgtgt aaggggtctg gcctctccga
     4741 gagccgcgcc gtgcggcgtc cggtacgccg cggtctcgaa cgcggatcga ggacaggcct
     4801 gaccccgatc cccggctgac gatcggggtc aggcctgtcc tcgcatcgcg gcggcggcgt
     4861 ggctccgctg cgcaccatgg cgcgagcaag gagaggccag acccctttgc gcccgcgtca
     4921 cgctcaggcc acgaattcgt tgaccaggtt ctcccacgcc tcctggcgcc cggacaccgg
     4981 ccgctcgtcg acgttgcgct ggagggcatg ggacgcggcg tcgtcgaggg acgtcccggg
     5041 cgacagcagc ttgcggccga cgtcgccgtc ccagcccgcg tagcgcgcgt ccagatcgcg
     5101 cgccaggcgg ccgtcggtga ccatgcgttc ggccgccagc agcgcacggg cgcacaggtc
     5161 catcgcgccg acgtggccgt ggaacaggtc ggccggatcg atcgactggc ggcgcacctt
     5221 ggcgtcgaag ttcagcccgc cgtgcgtgag gccgccaccg cgcagcacgt ggtacagcgc
     5281 gagcgccgtc tcgggcaggt tgttggggaa ctggtcggtg tcccagccgc actgcatgtc
     5341 gccgcggttc atgtccagcg atccgaacag gccgagggcg acggcagtgg cgatctcgtg
     5401 ctcgaagctg tggcccgaca gcgtcgcatg gttggcctcg atgttgacct tgacctcgtt
     5461 ctccagcccg tactggcgca ggaagccgtg cacggtggcg acgtcgtagt cgtactggtg
     5521 cttggtgggt tcgcgcggct tgggctcgat cagcacgtag ccgggcaggc cgagcttgtg
     5581 cttgtgctcc acggccatct gcaggaagcg gccgtactgg cgcgactcgc gcgccaggtc
     5641 ggtgttgagc agcgtctcgt agccctcgcg gccgccccac agcacgtagt tggcgccgcc
     5701 caggcgcctg gtcgcgtcca tcgcttcctt gacctgcagc gcggcgagcg cgtagatctc
     5761 gggattcggg ttcgtcgccg cgccgctcat gaagcgccgg tggctgaaca ggttggccgt
     5821 gccccacagc agcttgacgc cggtggcggc ctgcttctcg cccatgaggt cgacgatgcg
     5881 gcggaagttg tcgacgctct cccgcggcgt gctgccttcg ggggcgacgt cgcggtcgtg
     5941 gaagcagtag aacggcgcgc ccagcttggc gaagaactcg aaggcgacgt cgagcttggc
     6001 cttggccgct tccatcggat cggccacgcg atgccacggg cgatcgaacg tgccgtcgta
     6061 gccgaacgga tcgaacccgt tccagcagaa cgagtgccag tagcagacgg cgaagcgcag
     6121 ctggtcctcc atgcgcctgc ccaggacgat gcggtccttg tcgtaccact tgaaggacag
     6181 cgggtcggtc gattgcgggc cggcgtagcg gatcttgtcg atgccggcga agtagtcgga
     6241 catggcgggg tctcctggag ggaaggaatc agtgacgcgg catcagctcg cgggtgtggc
     6301 gatagagcgc gcggaagcgt tcgaggcggg cctgcagcgc ggggcggcgc gctgcgcgcg
     6361 gctggaagag ggcgcgcggc gccggggggc ggcagacctc gcgttcatcg cggcccagcg
     6421 cgagccaggc caggcggccg gcgcccatcg cgccgccggc ttcggccccg acgacggcgg
     6481 gcggctggcc gcgatgtac
//
LOCUS       DATCRO010000950         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_33469, whole genome shotgun sequence.
ACCESSION   DATCRO010000950 DATCRO010000000
VERSION     DATCRO010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33469"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VIN75_25975"
     CDS             complement(<1..626)
                     /locus_tag="VIN75_25975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977689.1"
                     /translation="MRQLILDLAWDPVPAFAGFLPGDNAAVVAELREQSWPGAPVYLW
                     GPAGAGKTQLLRELQARAIEAGQVAQWFDAASSTPWNLAEDAALVLLDGVDQWTAEQQ
                     HAAFLLFVEAASRAGGVLDALADGVAGAALALAETSASGVRLGVQFASAGRFPPVDLQ
                     VREDLRTRLGWGPVHQVHPLAEAGTRDALHQEARRRGIVLGDEVLDYIL"
     gene            complement(685..1743)
                     /locus_tag="VIN75_25980"
     CDS             complement(685..1743)
                     /locus_tag="VIN75_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8977690.1"
                     /translation="MTLSPAQQRALTWLALAVAAFLLLWVLGPALSPLILALVFAYLL
                     WPMVRMLERRARLPRALATTVTVLFAMAAVTALALLLVPIVTTALPQLRAQWPQLLDK
                     FNHVVAPRLQELGIAVDTDTVKASILKSFDGNMEEWGQKLLLSARIGGSWLATLVGFL
                     VLVPVLVFYLLVDAESVTAGAWNLVPLRSRERLAAFLRECDTMLRQYLKGQLAVMVAM
                     ALIYPAGLMLTGLNLAWPIGVFTGLAVFIPYLGYTTGLVLALMSAAIQFTGWHGVAAV
                     AAVYGIGQVLESFVLTPRLVGERIGLSPLAVILALLVFGEVFGFVGVLVCLPVTALMV
                     VALRHAMAAYRASGFYRT"
     gene            complement(1891..2760)
                     /locus_tag="VIN75_25985"
     CDS             complement(1891..2760)
                     /locus_tag="VIN75_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY8977691.1"
                     /translation="MAPVPRPTPTFNAPSALEYFDALVADDEQFPLLEAAIAIAQDDR
                     PGLDVESVLADVDALALRLKNRLAADAPVTHKLRTLNRYFFEELGFAGNVNDYHDPAN
                     SYVPDVLARRRGIPITLALLYMEIAGHIGLRAEGVSFPGHFLVKLRLPQGEVVIDPFT
                     GQSLSRSDLEERLDLVRQASAMRDAFDVPLGLFLQAAGPRDIIGRMLRNLRQIHQGRG
                     DVPRLLAVLDRQLVLEPEDWDTYRLRGDLLDGLGRTEEAAVDWGLYLEHTPDAADADV
                     LQRRILAARSQRH"
     gene            complement(2879..4438)
                     /gene="murJ"
                     /locus_tag="VIN75_25990"
     CDS             complement(2879..4438)
                     /gene="murJ"
                     /locus_tag="VIN75_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8977692.1"
                     /translation="MNLLKAASTVSLLTLASRVSGLVRDQLIAASFGASGFTDAYNVA
                     FRIPNLLRRLFAEGAFSQAFVPLLANTRISEGDAATHKLVDAVATVLGWVLLVTCVIG
                     VLAAPALVWALASGLERFDAAVVMTRVMFPYIGFMSMVALSAGILNTWGRFSIPAATP
                     ILLNIAIIVAARGLTSTFEAHGLPGVYALSVGVMLGGVLQAAIQIPALKALGLMPHIR
                     TTWRGLVEARHHPGVGRVLKQMSPALLGVSVAQVSLLINTQIASHLATKSVSWLTYAD
                     RLMEFPTSLLGVALGVVLIPQLSAARARGDKEGYAGMIDWGLRLVVLQTLPCAIALLV
                     FARPLLAVLFHYGHFQAADVLGTVPALEGYGLGLLGIVSVKILAPGYFAQLDTRTPVR
                     IAIAVLVFTQLMNLLFVFGLHIGVGGLALSIGLGASLNACWLLVGLIRLGVYKPQPGW
                     FGFLARALVATALLGACLVWADHSIDWLDRSHPMLRAAKMAGVLGGVALLYVAVLSVM
                     GIKLRQFVRKA"
BASE COUNT          775 a         1677 c         1681 g          559 t
ORIGIN      
        1 aggatgtagt ccagcacctc gtcgcccagc acgatgccgc ggcggcgcgc ctcctggtgc
       61 agcgcgtcgc gcgtgccggc ctcggccagg ggatgcacct ggtgcaccgg gccccagccc
      121 aggcgcgtgc gcaggtcctc gcgcacctgc aggtcgaccg gcgggaagcg gccggcggag
      181 gcgaactgca cgcccaggcg cacgcccgac gccgaggttt cggccagggc cagcgcggcg
      241 ccggcgacgc cgtcggccag cgcgtcgagc acgccgccgg cgcggctggc ggcctccacg
      301 aacaacagga aggccgcgtg ctgctgctcc gccgtccact ggtcgacgcc gtccagcagc
      361 accagcgcgg cgtcctcggc caggttccac ggcgtcgacg acgcggcgtc gaaccactgc
      421 gccacctggc ccgcctcgat cgcgcgcgcc tgcagctcgc gcaacagctg cgtcttgccg
      481 gcgccggcgg gcccccacag gtagaccggc gcgccgggcc acgactgttc gcgcagctcg
      541 gcgaccaccg cggcgttgtc gccgggcagg aaaccggcga acgcgggcac cggatcccag
      601 gcgaggtcga ggatcagctg tcgcatgcga ccatcctgcg tgctgcgcac tccggcattc
      661 gcgcttcgga tcggccgtgc gcgctcatgt ccggtagaac ccactggcgc ggtaggccgc
      721 catcgcgtgg cgcagcgcca ccaccatcag cgccgtcacc ggcaggcaca cgagcacgcc
      781 gacgaagccg aacacctcgc cgaacaccag cagcgccagg atgaccgcca gcgggctcag
      841 gccgatgcgc tcgcccacca ggcgcggcgt gagcacgaag ctctcgagca cctggccgat
      901 gccgtagacg gccgcgaccg ccgccacgcc gtgccagccg gtgaactgga tcgcggccga
      961 catcagcgcc agcaccaggc cggtggtgta gcccaggtac gggatgaaca cggccaggcc
     1021 ggtgaacacg ccgatcggcc acgccaggtt caggcccgtc agcatcaggc cggccgggta
     1081 gatcagcgcc atcgccacca tcacggccag ctggcccttg aggtactggc gcagcatcgt
     1141 gtcgcactcg cgcaggaagg cggccaggcg ctcgcgcgag cgcaacggca cgaggttcca
     1201 ggcgccggcg gtcacggact cggcgtcgac cagcaggtag aacaccagca ccggcaccag
     1261 caccaggaag cccacgaggg tggccagcca gctgccgccg atgcgcgccg acagcagcag
     1321 cttctggccc cactcctcca tgttgccgtc gaaggacttc aggatcgacg ccttgaccgt
     1381 gtcggtgtcc accgcgatgc ccagctcctg caggcgcggc gcgacgacgt ggttgaactt
     1441 gtccagcagc tgcggccatt gcgcgcgcag ctgcggcagc gcggtcgtga cgatgggaac
     1501 cagcagcagc gccagcgccg tgaccgcggc catcgcgaac agcacggtga ccgtcgtggc
     1561 cagcgcccgc ggcagccggg cgcggcgctc gagcatgcgc accatcggcc acagcaggta
     1621 ggcgaacacc agcgccagga tcagcgggct cagcgccggg cccagcaccc acagcagcag
     1681 gaacgccgcc acggccaggg cgagccaggt cagagcgcgt tgctgggcgg gggacagggt
     1741 catgtagggt cgggggcggc gtggaaaccg gccatcgcga agcggacgcc ggtcgcggcc
     1801 cggaaaaggc cgccgcaggg gaagcgcggc ctcgagcgga ctgcaaccgc gcattgtagg
     1861 gcgcccgggc tcccctgccc cgcgccgccg tcagtggcgt tgcgagcgcg ccgccaggat
     1921 gcggcgctgc agcacgtcgg cgtccgcggc gtcgggggtg tgctccaggt acaggcccca
     1981 gtcgacggcg gcctcctccg tgcggcccag gccgtcgagc aggtcgccgc gcaggcggta
     2041 cgtgtcccag tcctcgggct cgagcaccag ctggcggtcg agcacggcca gcaggcgcgg
     2101 gacgtcgccg cggccctggt ggatctggcg caggttgcgc agcatgcggc cgatgatgtc
     2161 gcgcgggccg gcggcctgca ggaacaggcc caggggcacg tcgaaggcgt cgcgcatggc
     2221 gctggcctgg cgcacgaggt ccaggcgctc ctccaggtcg ctgcgcgaca gcgactgccc
     2281 cgtgaacggg tcgatcacga cctcgccctg cggcagccgc agcttgacca ggaagtggcc
     2341 aggaaacgac accccttcgg cgcgcagccc gatgtgaccg gcgatctcca tgtacagcag
     2401 cgccagcgtg atggggatcc cgcgccggcg cgccagcacg tccggcacgt agctgttggc
     2461 cgggtcgtgg tagtcgttga cgttgccggc gaagcccagc tcctcgaaga agtagcggtt
     2521 gagcgtgcgc agcttgtgcg tgacgggcgc atcggccgcc agccggttct tcaggcggag
     2581 cgccagcgcg tcgacgtcgg ccaggaccga ttcgacgtcc agtcctggac ggtcgtcctg
     2641 cgcgatggcg atggccgcct ccagcagcgg gaactgctcg tcgtcggcca ccagcgcgtc
     2701 gaaatactcc agcgcgctcg gcgcgttgaa ggtcggcgtg ggacgcggaa ccggggccat
     2761 ggggtgagtg tagacccgtg gagttgcggg tttcttgccc aaaacccgaa ggggtcggtg
     2821 ccacccaggc ggtcaccgct gcgcgcagcg aagcggagtc gcaggatgac ggcccgggtc
     2881 aggccttgcg cacgaactgc ctgagcttga tgcccatcac cgacagcacc gccacgtaca
     2941 gcagcgccac gccgcccagc acgccggcca tcttggccgc gcgcagcatc gggtgcgagc
     3001 ggtccagcca gtcgatcgag tggtcggccc agaccaggca ggcgcccagc aacgccgtgg
     3061 cgacgagcgc gcgcgccagg aagccgaacc agcccggttg cggcttgtag acgcccaggc
     3121 ggatcaggcc caccagcagc cagcaggcgt tgagcgaggc gcccaggccg atcgacagcg
     3181 cgaggccgcc cacgccgatg tgcaggccga acacgaacag caggttcatc agctgcgtga
     3241 acaccagcac ggcgatggcg atgcgcaccg gggtgcgggt gtccagctgc gcgaagtagc
     3301 cgggcgccag gatcttcacg ctgacgatgc ccagcagccc caggccgtag ccttccagcg
     3361 ccgggacggt gcccagcacg tcggcggcct ggaagtggcc gtagtggaac agcacggcca
     3421 gcagcggccg cgcgaacacg agcagcgcga tcgcgcacgg cagcgtctgc agaacgacca
     3481 ggcgcaggcc ccagtcgatc atgccggcgt agccttcctt gtcgccgcgc gcgcgcgccg
     3541 ccgacagctg cgggatcagc accaccccca gcgccacgcc cagcagcgag gtggggaact
     3601 ccatcagccg gtcggcgtac gtcagccagg acaccgactt ggtggccagg tgcgacgcga
     3661 tctgcgtgtt gatcagcagc gacacctgcg ccaccgacac ccccagcagc gccggcgaca
     3721 tctgcttgag cacgcggccc acgcccggat ggtggcgcgc ctcgacgagg ccgcgccagg
     3781 tcgtgcggat gtgcggcatc agcccgagcg ccttgagcgc cgggatctgg atggccgcct
     3841 gcagcacgcc gcccagcatc acgccgaccg acagcgcgta gacgcccggc aggccgtgcg
     3901 cctcgaacgt ggacgtgagg ccgcgcgcgg ccacgatgat ggcgatgttg agcaggatcg
     3961 gcgtggccgc ggggatcgag aagcggcccc aggtgttgag gatgccggcc gacagcgcca
     4021 ccatcgacat gaagccgatg tacgggaaca tgacgcgcgt catcacgacc gccgcgtcga
     4081 agcgctccag gcccgaggcc agcgcccaga ccagcgccgg cgccgccagc acgccgatca
     4141 cgcaggtgac gagcagcacc cagcccagga cggtcgcgac tgcgtcgacc agcttgtgcg
     4201 tggcggcgtc gccctcggag atgcgcgtgt tggccagcag cggcacgaag gcctgcgaga
     4261 acgcgccttc ggcgaacagc cggcgcagca ggttggggat gcggaaggcg acgttgtagg
     4321 cgtcggtgaa gccgctggcg ccgaagctcg cggcgatcag ttggtcacgc accaggccgg
     4381 acacgcgcga ggcgagcgtc agcagcgata ccgtcgaagc ggccttgaga aggttcatcg
     4441 ggggccgcgg tcgcttttgg cgcaggcggt tgagctggtc aggccggcga ttataggaag
     4501 caccctgtcg ggacaaacgt ggaatccgcg ggttatcccc cttttcggcc gaccaccccg
     4561 gcggctaccg atacaatgcc ggcatccgct ccctgatcca gtcccgagag cggccgtcga
     4621 tcctcgacgt cttctccagc agtcggcctt cacgactggt gcccgctgaa agtgcgcggc
     4681 gcagcaggcc ga
//
LOCUS       DATCRO010000951         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_652244, whole genome shotgun sequence.
ACCESSION   DATCRO010000951 DATCRO010000000
VERSION     DATCRO010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652244"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VIN75_25995"
     CDS             complement(<1..1415)
                     /locus_tag="VIN75_25995"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY8977693.1"
                     /translation="MHTVLLLDADSRVGTACLQSLGPAGHRVHVAVRRLGAPAESSRW
                     CHRVHVQPVAEPVDAGVAWLSELDARFGFALVVPTTEASLRWLRAMPEDHPVRRKAVL
                     PGDAALDAALDKSRTGRIARELGLPVPATRELAQCPRDVPWPEETRRPPAAWPVVLKP
                     VRSKVVRGGKLASLAVAVVHDEATRALTLSSWLPDTPVQEQAWVPGRGIGVEVLYEHG
                     RMAWHFVHERLHEWPLTGGASTLRRAAGPEPELVEMTRRLLDHLQWHGVAMVEWRRDA
                     GGAVHLMEINPRLWGSLPLTIAAGVPVPLGLLSLATGEPLAPVRGWRVGVTARNLSND
                     IQWFIDNLRANRRDPMLLTSSPWRAALGWLRVLTGRETWDGWSWRDPAIAREELSHLV
                     HKRFAGIAERVARRAALSRARRHHATLRRESGALARPVGSVLFLCLGNLCRSPFAEVA
                     AAQRLAGVAIASAGFLSHDGRP"
     gene            complement(1460..2392)
                     /locus_tag="VIN75_26000"
     CDS             complement(1460..2392)
                     /locus_tag="VIN75_26000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8977694.1"
                     /translation="MIFARQIHSFAFSTGLSTLFARSRRAYRVIMFHGVGDTHMPVEA
                     FEANLRWMAERFHVVPLADIVQRIEQGRMPDARGEVALTFDDGLGNHFKQAWPVLRRL
                     GLPASFFICPDLIDGRRWIWNQEARARLKRMTAAERGRFAQAEMRMQTDAIEPLVQRM
                     KQLALADRERVEAALRARTPDFEPTPQEHEHYDPITWEQVRELDPSLITIGSHTLSHP
                     ILPTIGDAALEHEVNESRRVLEARLGRPVDLFCYPNGSHDERVYAHVSRSYRAAITTN
                     YGLARPSSDLHRLMRIPAAANLPLMAWRMHWPNG"
     gene            complement(2389..>3064)
                     /locus_tag="VIN75_26005"
     CDS             complement(2389..>3064)
                     /locus_tag="VIN75_26005"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8977695.1"
                     /translation="RLWPARLPEFDVCAVQLPGRANRLFEPALTDVHAVVEALVDAVR
                     PLLDRPYVLFGHSMGTLVACLLARRLRALGAPLPERLFVSGRQPPHRPFPELAMDGLT
                     DAQVLDAVQRHFGGLPAEVLETPELLELVLPTLRADFALLAGHRDTAADPLPLPIVTL
                     GGDDDPWAAPERLAPWQACTSLPLRTHRFAGGHFYLDDRLDEVLAVLRAECAAPAASA
                     MAGGLA"
BASE COUNT          437 a         1080 c         1181 g          366 t
ORIGIN      
        1 gggcggccgt cgtgcgacag gaagcccgcg ctggcgatcg ccacgccggc caggcgctgg
       61 gccgccgcga cctcggcgaa cgggctgcgg cacaggttgc ccaggcacag gaacagcacc
      121 gagccgacgg gccgggcgag cgcgcccgat tcgcggcgca gcgtggcgtg gtgcctgcgc
      181 gcgcgcgaca gcgcggcgcg ccgggcgacg cgctcggcga tgcccgcgaa gcgcttgtgc
      241 accaggtgcg agagctcctc gcgcgcgatg gccgggtcgc gccaggacca gccgtcccag
      301 gtctcgcggc cggtcagcac gcgcagccag ccgagcgcgg cgcgccaggg cgaggacgtc
      361 agcagcatcg ggtcgcgccg attggcgcgc aggttgtcga tgaaccactg gatgtcgttg
      421 gacaggttgc gcgccgtcac gccgacgcgc cagccgcgca cgggcgcgag cggctcgccg
      481 gtggccagcg acagcaggcc cagcgggacc ggcacgccgg cggcgatcgt cagcggcagc
      541 gagccccaca ggcgcggatt gatctccatc aggtggacgg cgccgccggc gtcgcgccgc
      601 cattcgacca tcgcgacgcc gtgccactgc aggtgatcga gcagccgccg cgtcatctcc
      661 accagctccg gttcgggccc ggccgcgcgg cgcagcgtgc tcgcaccacc ggtcagcggc
      721 cactcgtgca ggcgctcgtg gacgaagtgc cacgccatgc ggccgtgctc gtacagcacc
      781 tcgacgccga tgccgcggcc cggcacccag gcctgctcct gcacgggcgt gtccggcagc
      841 caggacgaca gcgtcagcgc gcgcgtcgcc tcgtcatgca cgacggccac ggccagcgac
      901 gccagcttgc cgccgcgcac caccttgctg cgcacgggct tgagcaccac gggccaggca
      961 gccggcgggc ggcgcgtttc ctcgggccac ggcacgtcgc gcggacactg cgccagctcg
     1021 cgcgtggccg gcacgggcag gccgagctcg cgggcgatgc gtcccgtgcg cgacttgtcc
     1081 agcgcggcat cgagcgcggc gtcgccgggc agcaccgcct tgcggcgcac cgggtggtcc
     1141 tcgggcatcg cgcgcagcca gcgcagcgag gcctcggtgg tgggcaccac gagtgcgaag
     1201 ccgaagcgcg cgtccagctc ggacagccag gcgacgccgg cgtcgacggg ctcggccacc
     1261 ggctgcacgt ggacgcgatg gcaccagcgc gagctctcgg ccggcgcgcc caggcggcgc
     1321 acggcgacat gcacgcgatg gccggcgggg ccgagcgact gcaggcaggc ggtgccgacg
     1381 cggctgtcgg cgtccagcaa gaggacggta tgcacgatct gggaccggtg ggatgaacgc
     1441 ggccaggatt atgcgtgcgt cagccgttgg gccaatgcat gcgccaggcc atcagcggca
     1501 ggttggccgc cgcggggatg cgcatcaggc gatgcaggtc gctggagggc cgggccaggc
     1561 catagttggt ggtgatggcc gcgcggtagc tgcgcgagac gtgcgcgtac acgcgctcgt
     1621 cgtgcgatcc gttcgggtag cagaacaggt cgacggggcg tccgaggcgc gcctcgagca
     1681 cgcgccggct ctcgttgacc tcgtgctcga gggcggcgtc gccgatcgtc ggcaggatcg
     1741 ggtggctcag cgtgtgcgag ccgatggtga tcagcgacgg atcgagctcg cgcacctgtt
     1801 cccaggtgat cgggtcgtag tgttcgtgct cctgcggcgt cggctcgaag tccggcgtgc
     1861 gcgcgcgcag cgcggcctcg acgcgttcgc ggtcggcgag cgcgagctgc ttcatgcgct
     1921 gcaccagggg ctcgatggcg tcggtctgca tccgcatctc ggcctgcgcg aaccggccgc
     1981 gctcggcggc cgtcatgcgc ttgagccgcg cgcgcgcctc ctggttccag atccagcgcc
     2041 ggccgtcgat caggtcgggg cagatgaaga acgacgccgg caggcccaac cgcctgagca
     2101 ccggccaggc ctgcttgaaa tggttgccca ggccgtcgtc gaaggtcagg gccacctcgc
     2161 cgcgcgcgtc gggcatgcgg ccctgctcga tgcgctgcac gatgtcggcc agcggcacga
     2221 cgtggaaacg ctcggccatc cagcgcaggt tggcctcgaa ggcctcgacg ggcatgtgcg
     2281 tgtcgccgac accatggaac atgatgacgc ggtaggcccg gcggctgcgc gcgaacagcg
     2341 tcgacagccc cgtggagaac gcgaacgaat ggatctgccg cgcgaagatc atgcgaggcc
     2401 acccgccatc gcgctggcgg ccggcgcagc gcattcggcg cgcagcacgg ccagcacctc
     2461 gtcgagccgg tcgtcgaggt agaaatgccc gccggcgaac cggtgcgtgc gcagcggcag
     2521 cgacgtgcag gcctgccacg gggcgaggcg ctccggcgcg gcccagggat cgtcgtcgcc
     2581 gccgagcgtg acgatgggca acggcagcgg gtcggccgcg gtgtcgcggt ggcccgcgag
     2641 cagggcgaag tcggcgcgca gcgtgggcag cacgagctcc agcagttcgg gcgtctccag
     2701 cacctcggcg ggcaggccgc cgaaatggcg ctgcaccgcg tcgagcacct gcgcgtcggt
     2761 caggccgtcc atggccagtt cggggaacgg ccggtgcggc ggctggcgcc ccgagacgaa
     2821 cagccgttct ggcagcggcg cgcccagcgc gcgcaggcgt cgcgcgagca ggcatgcgac
     2881 gagcgtgccc atgctgtggc cgaagagaac gtaggggcga tcgagcagcg gccgcacggc
     2941 gtcgaccagc gcttcgacga cggcatggac atcggtcagt gccggctcga acagccggtt
     3001 cgcgcggccg ggcagctgca ccgcgcagac atcgaactcg ggcaggcgcg caggccacag
     3061 gcgg
//
LOCUS       DATCRO010000952         9743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_596224, whole genome shotgun sequence.
ACCESSION   DATCRO010000952 DATCRO010000000
VERSION     DATCRO010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596224"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VIN75_26010"
     CDS             <1..930
                     /locus_tag="VIN75_26010"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEY8977696.1"
                     /translation="VSALSTDTLFELPTVGDAELIGVFEHGSPEWHAARMDGIGGSEI
                     SAVLDISIWESPFSLWHRKAGAVPPREANTEMEAGRRLEPVICEVFAERHPDLETRRG
                     GTFRNRQRRWQIANPDQLVYENGRLTAIHEAKFALYPEKFGEEGTDQVPPYYQAQCRW
                     YADTFGITRTYLTAFVGSTGEFREYVIDADADDTALMRERAEAFMASVAAGIRPPIDG
                     HDATYRMVKDLPDGLDDIDVEVPVPMRDEYFDALARYAAAKTEKQRAASVVLDAIGTG
                     RRAVCFTDPVATRTVRDGKTYSLQPARNRGTAA"
     gene            927..1802
                     /locus_tag="VIN75_26015"
     CDS             927..1802
                     /locus_tag="VIN75_26015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEY8977697.1"
                     /translation="MTTNTVSGAVAVRENGPSAMIEQYKGDLAMVMPSHVKPDTFVRL
                     AVSVLRRDPNIAAAAANNPAALMGALMEAARLGLEPGTEQYYLTVRKVKGVPEIQGIR
                     GYQGEIELIYRAGAVSSVIVEVVRERDLFKYAPGRDERPLHEIDWDADRGALRLVYAY
                     AIMRDGATSKVVVLNRAKVMEAKAMSKGADSPYSPWVNHEEAMWLKTAAHRLAKWVPT
                     SAEYMRERLRAHAEVAAEAGKPPLAAVSMPMPHPVAPPGEEVVEGHAHEDEFDPDCEA
                     CRKESAAADRLAAGQ"
     gene            1799..1927
                     /locus_tag="VIN75_26020"
     CDS             1799..1927
                     /locus_tag="VIN75_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977698.1"
                     /translation="MKTAVAVIVVAAVVAYAVWESPMVRRLRAERSLRKGRDRDAS"
     gene            1953..2204
                     /locus_tag="VIN75_26025"
     CDS             1953..2204
                     /locus_tag="VIN75_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977699.1"
                     /translation="MPPRTVVGRNPDVTENCATPGCGEVDAGAIAAAAGLPRPQVPRG
                     WIYVVVKASTLPAAWYCSPPCASRGIALAQLRLTPQLGK"
     gene            2281..3009
                     /locus_tag="VIN75_26030"
     CDS             2281..3009
                     /locus_tag="VIN75_26030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977700.1"
                     /translation="MTDHQTVSSRRLSRIIRQVIDHAGTDERSDLSVVRLESDGTHLY
                     AVATDRFTLAVARELLDRELNPDAQPFSVLLYPRDAAHLAKIADDEAFDDGQPWSAPT
                     DFYTRLLVSDGRPPIAAAQVLVRKEGDIEHRDDVSAKYVPAPDVLPYDWRDLVRRCLQ
                     TQSSESAAVRKMAFNPQLLARWRSSDIEPLHFRFTGALNAVIVTGHNFLGVQLPVKPT
                     DPSESFDAYASAWTIDLAPQAVDS"
     gene            3009..3665
                     /locus_tag="VIN75_26035"
     CDS             3009..3665
                     /locus_tag="VIN75_26035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977701.1"
                     /translation="MSFAAAFPDADIPRPADAHPDAGRHEHPTSDPQPPVGTLPTGPH
                     AEAEAAALQAARSGAELVGYATPDPHTGRLVRFGSILTPDQVGIIPAPLPSDRDPGEN
                     RRDADWPTPRPSYSNTSTGRVLAAIDDRIDDTLRQRNQALRTQLRGKTAELAQERSLR
                     QDAQARLASERDAARTRIAELELTVAALGEKLAKAEKLLGVVGAAVKHAIGHDKTGGE
                     "
     gene            3665..4438
                     /locus_tag="VIN75_26040"
     CDS             3665..4438
                     /locus_tag="VIN75_26040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY8977702.1"
                     /translation="MATPYFDDGRVQLWLGDCREILPQLNVFEPEHLIIADPPYGETR
                     HAWDAWPEGWLNAVAEVGRSLWCFGTLRMFGAHWAEYAAAGFKLSHDVIWQKHNPSGP
                     DADRFRRIHEQIAFFYRGAWRDVWHDPQRVTTGVVEHGRVVNQGAKAIGHRGAYASSG
                     WRDDGTRLMTSVIAMRSMHRMGNIAPTEKPVGLLAPLIRYGCPREGLVIDPFAGSGST
                     LAAAISEGRSAIGIEADEAQIERAAKRLSQGALDVGAAS"
     gene            4435..4749
                     /locus_tag="VIN75_26045"
     CDS             4435..4749
                     /locus_tag="VIN75_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977703.1"
                     /translation="MTTTAICLKGRQREVGPRLEWAPDIVYVGRQCTQGGWKLAASQF
                     ANPYRAQQVGGAAKAVELYREWAWPAGVGGGRAGDAAWQTARLLVHEGPTVPRLRARG
                     NR"
     gene            4762..4902
                     /locus_tag="VIN75_26050"
     CDS             4762..4902
                     /locus_tag="VIN75_26050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977704.1"
                     /translation="MTRRKRWRPPWPWLAVGTGFALLVGALALDGIGNVLILLARAVG
                     LR"
     gene            4899..5246
                     /locus_tag="VIN75_26055"
     CDS             4899..5246
                     /locus_tag="VIN75_26055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY8977705.1"
                     /translation="MSESPCPFCEIAAGRAPAAIVGDWPDALAIRPLNPVVPDHLLVI
                     PRVHVAHALTDPIVTGAVMARAAGLGQLIGCDLNLITSVGPAATQTVRHLHIHIVPRV
                     EGDGLALPWTGQS"
     gene            5363..5917
                     /locus_tag="VIN75_26060"
     CDS             5363..5917
                     /locus_tag="VIN75_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977706.1"
                     /translation="MPGRDYLAEMNAAIDAALPDGDYVAPLVAHDLVEKLLATDPDLL
                     HGWCEARYEPILADFIHHKVKARRSASREASARAAFAEAAGAFVASGDALALREAVRS
                     PFDAEYVVNGDLLRRKVRDMVGADHLFVASEYAESKQTAALLESFHRAVAKKVGGKRT
                     SEVFTEETYLAMYRSITRQDSVAA"
     gene            6227..6904
                     /locus_tag="VIN75_26065"
     CDS             6227..6904
                     /locus_tag="VIN75_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977707.1"
                     /translation="MASVFAQYIPQAWPYRYAGTLHIATLAGGTPSDPNVAEGWLRTK
                     LADKNDLIRDAVAEVMAERGITAEEAARELDRLKHLNGFRKDPERGGELYIEGRHLKA
                     GIKEAASVARAVGKLPDRWGKTNKGILGFVSEHIQVVEDRLHLGVTEASDVLQSFPKN
                     ARTGQTGIQYTEIVRDAKLDFTLITDHKFSDEEWAMLWLTAEQQGIGASRSQGFGRYE
                     VVRWGPA"
     gene            complement(6961..7209)
                     /locus_tag="VIN75_26070"
     CDS             complement(6961..7209)
                     /locus_tag="VIN75_26070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977708.1"
                     /translation="MFAVQEDLAARMKLSAARFRRTKTAYESARADLNSLMFDAQEEG
                     SGPSEIARATGFTREWVAKVIAAERKRRGLPAADASDS"
     gene            7440..7877
                     /locus_tag="VIN75_26075"
     CDS             7440..7877
                     /locus_tag="VIN75_26075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977709.1"
                     /translation="MAHFSVAPTPAAPKLRGHLRKGENRPAKTGRAARVRAGTFNSVA
                     RRADVLRRLAERVNTGRTTTATYLGTLGADEAFIASYASPYGRAVAKVYREKFGAEPE
                     RSGLAVRGRHLLRVFAYSADELPILDAAARAYNRTATLVGGAR"
     gene            7874..8221
                     /locus_tag="VIN75_26080"
     CDS             7874..8221
                     /locus_tag="VIN75_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977710.1"
                     /translation="MSDTIGVLMPDGIERPTCGEFYCVGHADETTTDTHCTPYEYFGE
                     FGVVVTRETGQQPKVSLVDLPHPQADFDLDEVPRLILQLCHALEIAQRAAMQPPAVQL
                     AEQVRALFPAATA"
     gene            8320..9198
                     /locus_tag="VIN75_26085"
     CDS             8320..9198
                     /locus_tag="VIN75_26085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977711.1"
                     /translation="MAVEVIEWVFKHSRSKNGARLTLLAIADACHSPEGTGAWPSMAE
                     LVEKTQLSERAVHDAIAVCVKLGELWVGLNQGPKGCNRYAVLMTGAKSAPPQNLHPAK
                     SAPPAESAPVHAPESSQVVQEKGADSAPPADIAPPQNLHPGGADSAPGTVREPSVDKE
                     EGTTSPPTRRRGAKRPPANTVAARFDDFWGVYPKREGKGAAEKAWTRAINTIGADPQA
                     VIDAALEYAQKRKGQDPQFTKQPATWLNARCWEDEPAPAYADSGMIPGFQAPRPNGYQ
                     PYRNPENHDVYDAWMG"
     gene            9201..9743
                     /locus_tag="VIN75_26090"
     CDS             9201..9743
                     /locus_tag="VIN75_26090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977712.1"
                     /translation="MTTDLHTETTVIDHPGTPNCPACKTGNPTVADVRRALVAESEEF
                     GTRRLPYSRALRGSFDATQADFDAYEALCAAGSFAFVLAAILRKLAEDHGEAYAAQFA
                     AVVHSAMADGPDGIDECANDDLGAARTTPTRTGNLPAAFDEGVSAFRGAGDCESCHGT
                     GLNIGQPGNCPACGGTGGSR"
BASE COUNT         1617 a         3569 c         3219 g         1338 t
ORIGIN      
        1 gtgtcggcgt tgagcactga caccctgttc gaactgccga ccgtgggcga cgccgaactg
       61 atcggcgtgt tcgagcacgg ctcgcccgag tggcacgccg cccgcatgga cggtatcggc
      121 ggcagcgaga tctccgccgt actcgacatc tcgatctggg agtcgccttt ctccctgtgg
      181 caccgcaagg ccggcgccgt cccgccgcgc gaagcgaaca ccgagatgga ggcgggacgc
      241 cgcctggagc cggtgatctg cgaggtattc gctgaacggc accccgacct ggagacgcgc
      301 cgcggcggca ccttccgcaa ccggcagcgc cgctggcaga tcgccaaccc ggaccagttg
      361 gtctacgaga acgggcggtt gaccgcgatc cacgaggcga agttcgccct ctacccggag
      421 aagttcggcg aggaaggcac cgaccaggtg ccgccctact accaggcgca atgccgctgg
      481 tacgccgaca ccttcggcat cacccgcacc tacctgaccg cgttcgtcgg ctcgaccggc
      541 gagttccgcg agtacgtcat cgacgccgac gccgacgaca ccgcgctgat gcgcgagcgc
      601 gccgaggcgt tcatggcctc ggtcgcggcc gggatccgtc ccccgatcga cggccacgac
      661 gccacgtacc gcatggtcaa agacctgccc gacggcctgg acgacatcga cgtcgaagtg
      721 cctgtcccga tgcgtgacga gtacttcgac gcgctcgcga ggtacgcggc ggcgaagaca
      781 gagaagcagc gtgccgcttc ggtcgtgctc gacgcgatcg gaaccggccg ccgcgcagtg
      841 tgcttcaccg acccggtcgc tacgcgcacg gttcgcgacg gcaagacgta cagccttcag
      901 cctgcacgaa acagggggac cgcagcatga cgaccaacac cgtaagcggc gccgtcgcgg
      961 tccgcgagaa cggccccagc gccatgatcg agcagtacaa gggcgacctc gcgatggtca
     1021 tgccgtcgca cgtcaagccc gacacgttcg tgcgcctcgc cgtctcggtg ctgcgccgcg
     1081 acccgaatat cgccgctgcg gcggcgaaca acccggccgc ccttatgggc gcgctgatgg
     1141 aggctgcccg gctcggcttg gagccgggca cggagcagta ctacctgacg gtccgcaagg
     1201 ttaagggtgt ccccgagatc cagggcatcc gcggctatca gggtgagatt gagctgatct
     1261 accgcgccgg cgcggtgtcc tcggtgatcg tcgaggtggt gcgcgaacgc gacctgttca
     1321 agtacgcgcc tggccgcgac gagcgcccgc tgcacgagat cgactgggat gcggaccggg
     1381 gcgccctgcg cctggtgtac gcctacgcga tcatgcggga cggtgcgaca tcgaaggtcg
     1441 tcgtgctcaa ccgggccaag gtcatggaag ccaaggccat gtcgaagggc gcggattcgc
     1501 cctactcgcc gtgggttaac cacgaagagg cgatgtggct gaagaccgct gcacaccgcc
     1561 tggcgaagtg ggtgccgacc tcggcggagt acatgcggga gcggttgcgc gcgcatgccg
     1621 aggtcgccgc cgaggcgggt aagccgccgc tggccgcggt gtcgatgccg atgccgcacc
     1681 cggtcgctcc acccggcgaa gaagtcgtcg aggggcacgc gcacgaggac gagttcgatc
     1741 cggactgcga ggcctgccgt aaggagtcgg ccgccgcgga ccgtctggcg gccggccagt
     1801 gaagaccgcc gtcgccgtga tcgtggtcgc cgccgtggtg gcgtacgcgg tgtgggagtc
     1861 gccgatggtg cgccgcctgc gcgcggagcg gagcctgcgc aaggggcgtg accgcgatgc
     1921 ctcgtaagcc gaatcccgat ccggaccgtg gcatgccgcc gcgcacggtg gtcggccgca
     1981 acccggacgt gaccgagaac tgcgcgactc cgggttgcgg cgaggtcgac gcgggcgcta
     2041 tcgccgccgc tgcgggcctt ccgcgcccgc aggtgccgcg cggctggatc tacgtcgtgg
     2101 tcaaggcctc gacgctcccc gcggcttggt actgctcccc gccgtgcgcg tcgcgcggta
     2161 tcgccctggc gcagcttcgg ctgacgcccc agctcggcaa gtaacccctg atctccctcc
     2221 cgcgcggtga gccgcactct cccgcgcggg aggggacacc aagactttaa ggacccgatc
     2281 atgaccgatc accagaccgt ttccagccgc cgcctctccc gcatcatccg gcaggtcatc
     2341 gaccacgcgg gcaccgacga gcgcagcgac ctgtccgtcg tccggctcga atcggacggc
     2401 acgcacctgt acgcggtggc caccgaccgc ttcaccctcg ccgtcgcccg cgaactgctc
     2461 gaccgcgagc tgaaccccga cgcgcagccg ttcagcgtcc tgctctaccc gcgcgacgcc
     2521 gcacatctgg cgaagatcgc cgacgatgag gcgttcgacg acggccagcc atggtccgcc
     2581 ccgaccgact tctacacgcg cctgctggtc agtgacggca gaccgccgat cgccgccgcg
     2641 caagtgctgg tccgcaagga aggggacatt gaacaccgcg atgacgtgag cgccaagtac
     2701 gtgcccgcgc ccgacgtgct cccgtacgac tggcgcgacc tggtgcgcag gtgcctccag
     2761 acgcagtcga gcgagtccgc ggcggtacgc aagatggcct tcaaccccca gctcttggcg
     2821 cgctggcggt cctcggacat cgagccgctg cacttccggt tcacgggggc gctcaacgcg
     2881 gtgatcgtga ccggccacaa cttcctcggt gtgcagctgc cggtcaagcc gaccgatccg
     2941 agcgagtcgt tcgacgcgta tgcctccgcc tggaccatcg acctggcgcc gcaggcggtg
     3001 gactcctgat gtccttcgcc gccgccttcc ccgacgcgga catcccgcgc cccgccgacg
     3061 cacacccgga cgccggccgc cacgagcacc cgaccagcga cccgcagccg cccgtgggca
     3121 ccctgccgac cggcccgcac gccgaggccg aggctgcggc gctccaggcc gcgcgcagcg
     3181 gcgcggagct ggtcgggtac gccacgcccg acccgcacac cggacgcctg gtcaggttcg
     3241 gctcgatcct gacgcccgac caggtcggca tcatcccggc cccgctgccg tccgaccgcg
     3301 accccgggga gaaccggcgc gacgcggact ggcccactcc ccggccgtcc tacagcaaca
     3361 cctcgaccgg ccgcgtgctg gccgcgatcg acgaccggat cgacgacacc ctgcggcagc
     3421 gcaaccaggc cctgcgtacg cagctgcgcg gcaagacggc ggagctggcg caggagcggt
     3481 cgctgcggca ggacgcgcag gcccggctcg cgtcagagcg ggacgcggca cggacgcgca
     3541 tcgccgaact ggaactgacc gtcgccgcgc tcggcgagaa gctcgcgaag gccgagaagt
     3601 tgctgggtgt cgtcggcgcg gcggtcaagc acgccatcgg gcacgacaag acgggcggcg
     3661 agtgatggcc acgccctact tcgacgacgg ccgggtccag ctgtggctgg gcgactgccg
     3721 cgaaatcctg ccgcagctga acgtgttcga acccgagcac ctgatcatcg ccgatccgcc
     3781 ctatggcgag actcgccacg cctgggacgc ctggcccgaa ggctggctca acgctgtcgc
     3841 cgaagtcggc cgatccctgt ggtgcttcgg cacgctgcgc atgttcggcg cccactgggc
     3901 cgagtacgcg gcggccggat tcaaactctc ccacgacgtg atctggcaga agcacaaccc
     3961 ctccggaccc gacgcggacc gcttccgacg catccacgag caaatcgcgt tcttctaccg
     4021 cggcgcgtgg cgtgacgtgt ggcacgaccc acagcgcgtc accaccggcg tggtggagca
     4081 cggtcgcgtg gtcaaccagg gcgcgaaggc gatcgggcat cggggcgcct acgccagcag
     4141 cggctggcgg gacgacggca cccgcctgat gacctcggtc atcgcgatgc ggtccatgca
     4201 ccgtatgggc aacatcgcgc cgacggagaa gccggtgggg ctgctcgccc cgttgatcag
     4261 gtacgggtgt ccgcgcgaag gcctggtgat cgacccgttc gcgggctccg gctccacgct
     4321 tgccgccgcg atctctgagg gccgctcggc gatcggtatc gaggcggatg aggcgcagat
     4381 cgagcgggcg gccaagcgcc tgagtcaggg cgcgctcgac gtcggagccg cctcgtgacc
     4441 acgaccgcga tctgtctcaa gggccgtcag cgtgaggtcg gcccgcgcct ggagtgggcg
     4501 ccggacatcg tgtacgtcgg gcgccagtgc acgcagggcg gctggaagct ggcggcgtcg
     4561 cagttcgcca acccgtaccg cgcgcagcag gtcggcggcg cggcgaaggc ggtcgagctg
     4621 taccgggagt gggcgtggcc ggccggagtt ggtggcggtc gcgcgggcga tgctgcgtgg
     4681 caaacggctc ggctgctggt gcacgaaggg ccaaccgtgc cacgcctacg tgctcgcgga
     4741 aatcgctgac ggagcgaacc cgtgacccgc cgtaagcgtt ggcgcccgcc gtggccgtgg
     4801 ctggccgtcg ggacggggtt cgcgctgctc gtgggcgctc tcgcgctgga cgggatcggg
     4861 aatgtgctga tcctgctcgc cagggcggtg gggctgcgat gagcgagagt ccgtgcccgt
     4921 tctgcgagat cgccgcagga cgcgcccccg ccgcgatcgt gggggactgg cccgacgcgc
     4981 tcgccatccg cccgctcaac cccgtcgtgc ccgaccacct gctcgtaatc ccccgcgtgc
     5041 atgtcgccca cgcgctgacc gacccgatcg tgacgggcgc cgtcatggcg cgggcggcgg
     5101 ggctcgggca gttgatcggc tgcgacctga atctgatcac ctcggtcggc ccggccgcca
     5161 cgcagaccgt ccggcacctg cacattcaca tcgtgccgcg cgtcgagggc gacggcttgg
     5221 cgctgccgtg gacggggcag tcgtgacccc cctcccccgt cccgcgcccg tcctcggcca
     5281 ccgtccgggg cgcacgcgcg cgggcacgtc agtcgacggc aggtgtcgct cccccctcac
     5341 caagcgcttc atggaggtcc ccatgcccgg ccgtgactac ctcgccgaaa tgaacgccgc
     5401 tatcgatgcc gcactgcccg acggggatta tgtggctccg ctcgtcgcgc acgacttagt
     5461 cgagaagctg ctggccaccg acccggatct actgcacggc tggtgcgagg cccggtacga
     5521 accgatcctc gctgacttca tccaccacaa ggtcaaggcg cgacgcagcg cgagccggga
     5581 ggcctcggcg cgtgccgcgt tcgccgaggc cgctggcgcg ttcgtggcgt ccggggatgc
     5641 gttggctctc cgtgaggccg tgcgcagccc gttcgacgcc gagtacgtgg tcaacggtga
     5701 cctgttgcgg cgcaaagtcc gcgacatggt cggcgcggat cacctgttcg tcgccagcga
     5761 gtacgccgag tcgaagcaga cggcggcgct ccttgagtcg tttcaccgcg cggtcgccaa
     5821 gaaggtcggc ggcaagcgga ccagcgaggt cttcaccgaa gagacctacc tggcgatgta
     5881 ccgatccatc acgcgccaag actccgtggc ggcgtgatgg acacccgcaa ccaccccctc
     5941 gcatcacctc tacgcgacat gccgacacca ctcgccacgt cccgcctcgc aacgactttc
     6001 ctagccctcg cgcgccgtca cgccccccgc ccctgccgac tacgccgccc aacacgcacc
     6061 ggcccagacc gtctcacccc accccgcgtc gacttcccga ccctggcctg ccccttcccg
     6121 accctggcct gcccgcaccg acttcccgaa tcgactaccc cgctcgattc ctcccggctc
     6181 agcccctttc gacccctccc gtcccatcga ctacccaagg actgatatgg cgtccgtctt
     6241 cgcccaatac atcccgcaag cgtggcccta ccgctacgcc ggaacactgc acatcgccac
     6301 gctcgccggc ggaaccccct ccgacccgaa cgtcgccgag gggtggctac gcaccaagct
     6361 cgccgacaag aacgacctca tccgcgacgc cgtcgccgaa gtcatggccg aacgcggaat
     6421 caccgctgag gaagctgcca gggaactcga tcgactcaag catctcaacg ggttccgcaa
     6481 ggaccccgag cgcggcggag agttgtacat cgaggggcgg catctcaagg ccgggatcaa
     6541 ggaggcagcg tccgtcgccc gcgctgtggg caaactgccc gatcggtggg ggaagacgaa
     6601 caagggcatc ctcggattcg tctccgagca catccaagtc gttgaggacc gactgcacct
     6661 cggcgtcacc gaggccagcg acgtactcca gtcgttcccg aagaacgcgc gcaccggcca
     6721 gaccggcatc cagtacaccg agatcgtccg ggacgcgaag ctcgacttca cgctgatcac
     6781 cgaccacaag ttcagcgatg aagagtgggc gatgctctgg ctgaccgccg agcagcaggg
     6841 aatcggggcg tcacggtcgc agggcttcgg ccggtacgag gtcgtgcggt ggggaccagc
     6901 gtgatctgtc cctcgtcgca cgcgagcccc ggtacgcgcg taccggggcg ctgccgcgcg
     6961 tcaggagtcg gaggcgtccg ctgcgggaag gccacggcgc ttccgctcgg ccgcgatgac
     7021 cttcgccacc cactctcgcg tgaaccccgt ggcacgcgcg atttcggacg gccccgagcc
     7081 ctcctcctgc gcgtcgaaca taagggaatt gaggtcggcg cgagcactct cgtaggcggt
     7141 cttggttcgc cgaaagcgcg ccgcgctcag cttcatacgg gcagcgagat cttcttgcac
     7201 ggcgaacatc ttgtcacctc tggtcgcgaa tggcatacgg gccggtgttc tcaatctagc
     7261 gcggaacagc ggtcgcgaac agtattgacg cgaagagtat gcgcgaatag tgttctcatc
     7321 gaagggtgcg gcgaagcggc aggcgtggac ctgcacccac agaagagacg gccccggcgg
     7381 gcgctaccaa cacccggcca gggcctgaac ccgcacctga gcaaccagga gacggatcta
     7441 tggctcactt tagcgtcgcc cccacccccg cagccccgaa gctgcgcggc cacctgcgca
     7501 agggcgagaa ccgcccggcc aagaccggac gcgccgcccg cgtccgcgcc ggcaccttca
     7561 acagcgtcgc ccgccgcgcc gacgtcctgc ggcgcctggc cgagcgcgtc aacaccggcc
     7621 gcacgaccac cgctacgtac ctcggcacgc tgggcgccga cgaggctttc atcgcctcgt
     7681 acgccagtcc gtacggccgc gctgtggcga aggtgtaccg cgagaagttc ggcgccgaac
     7741 cggagcgcag cggcctggcc gtgcgcggcc gccacctgct gcgagtgttc gcgtactcgg
     7801 ccgacgagct gccgatcctg gacgccgccg cccgcgccta caaccgcacc gcgaccctcg
     7861 tcggcggtgc ccggtgagcg acaccatcgg cgtcctgatg cccgacggca tcgagcggcc
     7921 cacctgcggc gagttctact gcgtcgggca cgcggacgag accacgaccg acacgcactg
     7981 cacgccctac gagtacttcg gcgagttcgg cgtggtcgtg acccgcgaga ccgggcagca
     8041 gccgaaggtc tcgctcgtgg acctgccgca cccgcaggcg gacttcgacc tcgacgaggt
     8101 tccccggctc atcctccagc tctgccacgc cctggagatc gcacagcgcg ccgccatgca
     8161 gccgccagcg gtgcagctcg ccgagcaggt acgggcactg ttccccgccg ccacggcctg
     8221 accggacgcg atcccggcgc tgacccgtcc cataccggcc ggcgtcagcg ccgggacgca
     8281 gcccacccat ccgagatcag cacgaacggg agacggcacg tggcagtgga ggtgatcgag
     8341 tgggtgttca agcactcccg ctcgaagaac ggcgcgcggc tgacgctgct cgccatcgcc
     8401 gacgcctgtc atagccccga aggcaccggg gcgtggccga gcatggccga gttggtcgag
     8461 aagacccaac tgtccgagcg cgcggtgcat gacgccatcg ccgtgtgcgt aaagctcggc
     8521 gagctgtggg tcggtctcaa ccaggggccc aagggatgca accgatacgc ggtgctcatg
     8581 accggtgcaa aatctgcacc cccgcagaat ctgcaccctg caaaatctgc accccccgca
     8641 gaatctgcac cggtgcacgc tccggagtca tcgcaggtcg tgcaggaaaa gggtgcagat
     8701 tctgcacccc ccgcagatat tgcacccccg cagaatctgc accccggggg tgcagattct
     8761 gcacccggaa ccgtaagaga accgtcagta gataaagaag aaggaactac ttctcctcca
     8821 acgcggcgac gcggcgccaa gcgcccgccc gcgaacacgg tcgccgcacg cttcgacgac
     8881 ttctgggggg tctaccccaa gcgcgaaggc aaaggcgccg ccgagaaggc ctggacccgc
     8941 gcgatcaaca cgatcggcgc tgacccacag gccgtcatcg atgcagcact cgaatacgcg
     9001 cagaaacgca agggccaaga cccgcagttc accaaacagc ccgccacctg gctgaacgcc
     9061 cgctgctggg aagacgagcc cgcccccgcg tacgccgaca gcggcatgat ccccggcttc
     9121 caggcgccac gccccaacgg ctaccagccc taccgcaacc ccgagaacca cgacgtttac
     9181 gacgcatgga tgggatgagc atgaccaccg acctgcacac cgaaacgacc gtcatcgacc
     9241 accccggcac gcccaactgc cccgcctgca agaccggcaa cccgaccgtc gccgacgtac
     9301 gccgcgcact ggtcgccgag tccgaggagt tcggcacccg ccgcctgccc tacagccgag
     9361 cgctgcgcgg cagcttcgac gccacccagg ctgacttcga cgcctacgaa gcgctgtgcg
     9421 ccgctggctc gttcgcgttc gtcctcgccg ccatcctgcg caagctcgcg gaggaccacg
     9481 gcgaggcgta cgcggcgcag ttcgctgccg tggtgcacag cgcgatggca gacgggccgg
     9541 acggaatcga cgagtgtgcc aacgacgacc tcggcgcggc caggaccacc ccgacgcgca
     9601 ccggcaacct ccccgccgcg ttcgacgagg gcgtgtccgc gttccgcggc gctggcgact
     9661 gcgagtcctg ccacggcacc ggcctgaaca tcggccagcc cggcaactgc ccggcatgcg
     9721 gcggaacggg cggctcgcga tga
//
LOCUS       DATCRO010000953         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1090673, whole genome shotgun sequence.
ACCESSION   DATCRO010000953 DATCRO010000000
VERSION     DATCRO010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090673"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1453
                     /locus_tag="VIN75_26095"
     CDS             545..1453
                     /locus_tag="VIN75_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977713.1"
                     /translation="MYNVVASLLVLTFLAGSARADDTSPSAPTRRDLLALAFPGWHPA
                     SLAEVPAGKPAIQRLRVPKVAWPFHGGIGDSTNQPRDIVKIAPLDVVRMDDTHAVLAT
                     WALSSDDHRAAACDSYYCTYALGLYFFEWADAGWHLRKRMDVATSLDGSAAPASRVQM
                     WPGRGAVLGISQENCHGGDCVDRLQLLGLETGRIGFRFGMATGIYEGDRFSPDPCSEV
                     VDPAYKPRPEENYANVNCRLAHGQWQVDGDTLRVDIEESSRKAHDDPQGNPVLGKLRI
                     QQRVVRLARQGSTLKVVDGKLPDYAL"
     gene            complement(1480..2904)
                     /locus_tag="VIN75_26100"
     CDS             complement(1480..2904)
                     /locus_tag="VIN75_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8977714.1"
                     /translation="MENWFSPELLALIREAEQPHATIALLLIAAAFPIAWGVPRLLRG
                     PEPRHGSIIFGGRIFDGVMFPALWLGLVWIARLFCLRQHLPLAVFKVVIPILLSLLVI
                     RLTMRVMRVAFPKSGVVRAVEKTVSWAAWLGVVLWITGVLPSFVDALDDIHWKMGAGT
                     VSLATILEGVIVAGVVLMVTLWVSAAIEARMLKGATGQRLSWRKAAANVVRAGLVFIG
                     LLLALSAVGIDLTALSVLGGAIGVGLGLGLQKLAANYVSGFVMLAEGALRIGDTVKVD
                     GFEGRISDITTRYTVLRALNGREAIVPNEMMVTQRVESSTLADPKMALSTVVQVGYDT
                     DLDTLVPRILALVPGIKRVIADPAPSVQLSAFASDGLELTISFWIADPENGTGSVKSD
                     INMALLRLFNAEGVEIPFPQRVVRTLAVAGGAEAQGVVPGAPSSRTLAASPAPAANGQ
                     ELQDAPPSKGQGPGLLEPGGAAGA"
     gene            3055..3543
                     /locus_tag="VIN75_26105"
     CDS             3055..3543
                     /locus_tag="VIN75_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977715.1"
                     /translation="MTTTVNPIPHGLHTITPHLVCKDASAAIEFYKEAFGATEVDRLA
                     GPGGKLMHAMLRIGDSAVMLVDEFPEMHALGPTSLGGTAVTLHLSVADADASFARATR
                     AGATARMPVTEMFWGARYGLVVDPFGHLWSIATQVKNLTMDQIRENLKSAPPMDGCGP
                     QG"
     gene            complement(3661..>4474)
                     /locus_tag="VIN75_26110"
     CDS             complement(3661..>4474)
                     /locus_tag="VIN75_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY8977716.1"
                     /translation="FLQPEPTIAQRIVRAKRTLGDANVPFEVPRGDALQARLSAVLEV
                     VYLIFNEGYSATAGDDWTRPALVEEGLRLARILAQLSPQTAEVQGLVALMEIQASRMR
                     ARLDAQGKPILLMAQDRSRWDQLLIGRGLAALARAEQLGDGPGPYQLQAAIAACHARA
                     RTPEATDWPRIVALYGELMTAAPSPVVELNRAVAVSMASGPAAALLLVDGLVTDGHLA
                     RYHLLHAARGDLLARLERHDEARVEFERAAAMTANEREKALLLERAREAAMR"
BASE COUNT          689 a         1641 c         1510 g          634 t
ORIGIN      
        1 cccccgcttc gccgtgccac gcacggcatg cccgcgttca acattcgtag gtggagatcg
       61 aggctttttt ggaggccttg gcctcgacgg cccatcgagg ccgcgtcgag gcccgcggcg
      121 agccgcccca cttgcggcgg gcttgacgcg aatgtcgggc cttgatgccg gcgctccttc
      181 tcatgacgga ccaacgggga gcatctgctc ggatggggtc aggtcttgaa agttgcgcga
      241 ccaagccgcg gctctcgtgg aatgcccgaa cgcgcaactt tcaagacctg accccatctt
      301 caatcgtgcc ggcgcgcaac gattcaagac ctgacaccgc aaatttagag acctgacacc
      361 gcgataccgt ggccgtcgcg ggtctttttt catggcgtgt tggccatcgg atcgaaccgc
      421 cggcagcgcc gaactcccag aagatgcacc gacatccagg gaagacttcc tctttcggtg
      481 acatcgacag cgccccgcct gcacaatcgg cggcccgcct gacgcgcccc aacgatccga
      541 tcgcatgtac aacgtcgtcg ccagtctcct cgtcctcact ttcctcgccg gttccgcccg
      601 ggccgacgac acgtccccgt ccgccccgac acgccgtgac ctgctcgccc tggcctttcc
      661 cggctggcac cccgcctcgc tggccgaggt gccggccggc aagccggcca tccagcgcct
      721 gcgagtgccc aaggtcgcct ggccgttcca cggaggcatc ggggacagca cgaaccagcc
      781 gcgcgacatc gtcaagatcg ccccgctgga cgtcgttcgg atggacgaca cgcacgcggt
      841 gctggcgacg tgggcacttt cgtcggatga ccaccgcgcc gccgcctgcg actcgtacta
      901 ctgcacctat gcgctgggcc tctacttctt cgaatgggcc gatgccggtt ggcatctgcg
      961 caagcgcatg gatgtcgcga ccagtctcga tggcagcgcc gcaccagcct cccgcgtcca
     1021 gatgtggccc ggacgtggcg ccgtgctcgg catcagccag gagaattgcc acggcggcga
     1081 ctgtgtggac aggctgcaac tgctcggcct ggagaccgga cggatcgggt tccgtttcgg
     1141 catggcgacc ggcatctatg aaggcgaccg cttcagcccg gacccgtgct cggaggtggt
     1201 cgaccccgcg tacaagccgc ggccggagga gaactacgcc aacgtgaact gccggctggc
     1261 gcacggccaa tggcaggtcg acggggacac cctgcgtgtc gatatcgaag aatcttcccg
     1321 aaaggcgcat gacgatccgc agggcaatcc cgtcctcggc aagctcagga tccagcaacg
     1381 tgtcgttcgc ctcgcccggc aggggtcgac gctgaaggtg gtcgacggca agctgcccga
     1441 ctacgctctt tgaacgtcgc gccgcgttcc cgcgcccgct caggcgccgg ccgcgccacc
     1501 cggctccagc agcccgggcc cctgtccctt cgacggtggc gcgtcctgga gctcctgccc
     1561 gttcgcggcc ggcgcggggg aggcggccag cgtccgcgaa gacggcgcgc ccgggacgac
     1621 gccctgcgcc tcggcgccgc ccgccaccgc cagcgtgcgc accactcgct gcgggaacgg
     1681 aatctcgacg ccctcggcat tgaacagccg cagcagcgcc atgttgatgt ccgacttcac
     1741 gctgcccgtc ccgttctccg ggtccgcgat ccagaacgag atcgtcagct ccaggccatc
     1801 cgaggcgaag gcgctcaact gcaccgaggg cgccgggtcg gcgatcacgc gcttgatgcc
     1861 gggcacgagc gcgaggatcc ggggcaccag cgtgtcgagg tcggtgtcgt agcccacctg
     1921 caccacggtg ctcagcgcca tcttcgggtc ggccagcgtg gagctctcga cgcgctgggt
     1981 caccatcatc tcgttgggca cgatggcctc gcggccgttg agcgcgcgca ggacggtgta
     2041 gcgggtggtg atgtcggaga tgcggccctc gaagccgtcc accttgacgg tgtcgccgat
     2101 gcgcagcgcg ccctcggcca gcatcacgaa gccgctgacg tagttggcgg ccagcttctg
     2161 caggcccagg cccaggccca cgccgatcgc gccgcccagc accgacagcg ccgtcaggtc
     2221 gatgcctacc gccgacagcg ccagcagcag gccgatgaac accagccccg cgcgcacgac
     2281 gttggccgcc gccttgcgcc acgacaggcg ctggcccgtc gcgcccttga gcatcctggc
     2341 ctcgatggcg gccgacaccc acagcgtgac catcagcacg acgccggcga cgatcacgcc
     2401 ctcgaggatc gtggccaggc tgaccgtgcc ggcgcccatc ttccagtgga tgtcgtccag
     2461 cgcgtcgacg aacgacggca gcacgccggt gatccacagc acgacgccca gccaggccgc
     2521 ccacgacacc gtcttctcga ccgcgcgcac gacgcccgac ttcgggaacg cgacgcgcat
     2581 cacgcgcatc gtcaggcgga tcaccagcag cgacagcagg atcgggatga cgaccttgaa
     2641 gacggccagc ggcaggtgct ggcgcaggca gaacaggcgg gcgatccaca ccaggcccag
     2701 ccacagcgcc gggaacatga cgccgtcgaa gatgcggccg ccgaagatga tcgaaccgtg
     2761 gcgcggctcg ggcccgcgca gcaggcgcgg gacgccccac gcgatcggga acgcggcggc
     2821 gatcagcaac agcgcgatgg tggcgtgcgg ctgctcggcc tcgcggatca gggcgagcag
     2881 ttcgggcgaa aaccagtttt ccatgcggcg attgtgcggg agcgcgacgg cggtgcggtg
     2941 acaggggcgc gggcgcgact attttgtccc gccttgtcga agccgcgcgc cgccgaacgt
     3001 cgtgtcgatg aagcctcgat ccgcttcagg catgttcaac cccaaggaga cgccatgacc
     3061 acgaccgtca acccgatacc gcacggcctg cacaccatca cgccgcatct cgtctgcaag
     3121 gacgcgtccg ccgccatcga gttctacaag gaggcgttcg gcgccaccga ggtggaccgc
     3181 ctcgccggcc ccggcggcaa gctgatgcac gcgatgctgc gcatcggcga ctcggcggtg
     3241 atgctcgtgg acgaattccc cgagatgcac gccctgggcc cgacctcgct cggcggcacg
     3301 gccgtcacgt tgcacctgtc cgtcgccgac gccgacgcat ccttcgcgcg cgccacccgc
     3361 gccggcgcca ccgcacgcat gcccgtgacc gagatgttct ggggcgcgcg ctacggcctg
     3421 gtggtcgacc cgttcggcca cctgtggtcg atcgcgacgc aggtcaagaa cctgacgatg
     3481 gaccagattc gcgagaacct gaagtcggcg cccccgatgg acggctgcgg cccgcagggc
     3541 tgatccccgc gcgcgggcgg tcgcgcgtga cgcgcgggcg ccgcgcgggc ctccctgcaa
     3601 gaatgacatc gccgcgcggg ccgcgggccg gcgcggcgtc cgccgtcgtc gaggcggcgc
     3661 ctagcgcatc gccgcctccc gcgcccgctc cagcagcagc gccttctcgc gctcgttggc
     3721 ggtcatcgcg gcggcgcgct cgaactcgac gcgcgcctcg tcgtggcgtt ccagccgtgc
     3781 cagcaggtcg ccgcgcgcgg cgtgcagcag gtggtagcgc gccaggtggc cgtcggtgac
     3841 gaggccgtcg accagcagca gcgccgcggc cgggcccgag gccatgctca cggcgaccgc
     3901 gcggttgagc tcgaccacgg gggacggcgc ggccgtcatc agctcgccgt acagcgcgac
     3961 gatgcgcggc cagtcggtcg cctcgggcgt gcgggcgcgc gcatggcagg ccgcgatcgc
     4021 cgcctgcagc tggtatgggc cgggcccgtc gccgagctgc tcggcgcgcg cgagcgcggc
     4081 caggccccgc ccgatcagca gttggtccca acggctgcgg tcctgggcca tcagcaggat
     4141 gggtttgccc tgcgcatcga gccgggcccg catgcgcgag gcctggatct ccatcaacgc
     4201 gaccaggccc tgcacctcgg ccgtctgcgg cgacagctgg gccaggatgc gcgccagccg
     4261 caggccttcc tcgaccagcg cgggccgcgt ccagtcgtcg ccggccgtgg cggagtagcc
     4321 ctcgttgaag atcaggtaga ccacctccag caccgcggac agccgcgcct gcagcgcatc
     4381 gccgcgcggc acctcgaacg gcacgttggc gtcgcccagc gtgcgcttgg cgcgcacgat
     4441 gcgctgggcg atggtgggct cgggctgcag gaag
//
LOCUS       DATCRO010000954         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_899522, whole genome shotgun sequence.
ACCESSION   DATCRO010000954 DATCRO010000000
VERSION     DATCRO010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899522"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2174)
                     /gene="rpoB"
                     /locus_tag="VIN75_26115"
     CDS             complement(<1..>2174)
                     /gene="rpoB"
                     /locus_tag="VIN75_26115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8977717.1"
                     /translation="EMKFIEYNIAKPPFDVRECQQRGLTFAAAVRAKLQMIIYDRESA
                     PAKVVKEIKEQEVYMGEVPLMTDYGSFIVNGTERVIVSQLHRSPGVFFEHDKGKTHSS
                     GKLLFSARIIPYRGSWLDFEFDPKDILYFRVDRRRKMPVTILLKAIGLNPEAILAHFF
                     KNDTFRLMDAGAQMEFVADRLKGEVARFDITDKDGNVVVEKDKRITARHVRQLEQTGT
                     THVTVPEDFLIGRVVARNMVDADTGEIIAKANDELTESLLKKLRGAGVKELAVLFTNE
                     LDQGAYISQTLATDETADQLAARVAIYRMMRPGEPPTEDAVEALFNRLFYSDDTYDLS
                     RVGRMKFNARINREGSEGAMTLSNEDIIAVVKILVELRNGRGEVDDIDHLGNRRVRCV
                     GELAENQYRSGLARIEKAVKERLGQAETEALMPHDLINSKPISAALKEFFGASQLSQF
                     MDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLI
                     NSLALYARLNDYGFLETPYRRVVDSKVTDQIDYLSAIEESKYVIAQANASLDSHGKLA
                     DELVSARENGESVLTSPERIQYMDVAPTQIVSVAASLVPFLEHDDANRALMGANMQRQ
                     AVPVLRPEKALVGTGVERVAAVDSGTVVTARRGGVVDYIDTNRVVIRVNDSETAAGEV
                     GVDIYNLIKYQRSNQNTNIHQRPIVQRGDKVGAGDVIADGAST"
BASE COUNT          313 a          665 c          782 g          414 t
ORIGIN      
        1 gtcgacgcgc cgtcggcgat cacgtcaccg gcgccgacct tgtcgccgcg ctggacgatg
       61 ggacgctggt ggatgttcgt gttctggttg gaacgctggt acttgatcag gttgtagatg
      121 tcgacgccga cttcgccggc cgccgtctcc gagtcgttga cgcggatgac gacgcggttg
      181 gtgtcgatgt agtcgaccac gccgccgcgg cgggcggtga cgacggtgcc cgagtcgacg
      241 gccgccacgc gctccacgcc cgtgccgacc agcgcctttt cagggcgcag gacggggacg
      301 gcctggcgct gcatgttggc gcccatcagc gcgcggttcg catcgtcgtg ctcgaggaac
      361 ggcaccagcg aggcggcgac cgacacgatc tgcgtcgggg cgacgtccat gtactggatg
      421 cgttccggcg aggtcagcac cgattcgccg ttctcgcggg cggagacaag ctcgtcggcc
      481 agcttgccgt gcgagtcgag cgaggcgttg gcctgcgcga tgacgtactt gctttcctcg
      541 atggccgaca ggtagtcgat ctggtcggtg accttggagt cgaccacgcg gcgatacggc
      601 gtctcgagga agccgtagtc gttcaggcgg gcgtacaggg ccagcgagtt gatcaggccg
      661 atgttcgggc cttccggcgt ctcgatcggg cacacgcggc cgtagtgggt cgggtgcaca
      721 tcgcggactt cgaagccggc gcgctcgcgg gtcaggccgc ccgggcccag ggccgagacg
      781 cgacgcttgt gcgtgatctc ggacagcggg ttggtctggt ccatgaactg cgacagctgc
      841 gacgcaccga agaactcctt gagcgccgcg gagatcggct tggagttgat caggtcgtgc
      901 ggcatcagcg cttcggtctc ggcctggccg aggcgctcct tgacggcctt ctcgatgcgg
      961 gcgaggcccg aacggtactg gttctcggcc agctcgccga cgcagcgcac gcggcgattg
     1021 cccaggtggt cgatgtcgtc gacttcgcca cggccattgc gcagttcgac gaggatcttg
     1081 acgacggcga tgatgtcctc gttggacagc gtcatcgcgc cttcggagcc ttcgcggttg
     1141 atgcgggcgt tgaacttcat ccggcccacg cgcgacaggt cgtacgtgtc gtcgctgtag
     1201 aacaggcggt tgaacagcgc ctcgacggcg tcctcggtcg gcggctcgcc ggggcgcatc
     1261 atgcggtaga tggcgacgcg cgcggccagc tggtcggcgg tctcgtcggt ggccagcgtc
     1321 tggctgatgt aggcgccctg gtcgagttcg ttggtgaaca ggacggcgag ttccttcacg
     1381 ccggcgccgc gcagcttctt gagcagcgac tcggtcagct cgtcattggc cttggcgatg
     1441 atctcgccgg tgtcggcgtc gaccatgttg cgggcgacga cgcggccgat caggaagtct
     1501 tccggcacgg tgacgtgcgt ggtgcccgtc tgctcgagct ggcgcacgtg gcgcgcggtg
     1561 atgcgcttgt ccttctcgac gacgacgttg ccgtccttgt cggtgatgtc gaagcgcgcg
     1621 acctcgccct tgaggcggtc ggcgacgaac tccatctgcg cgcccgcatc catcaggcgg
     1681 aaggtgtcgt tcttgaagaa gtgcgcgagg atcgcttccg ggttcaggcc gatggccttg
     1741 agcaggatcg tgaccggcat cttgcggcga cggtccacgc ggaagtacag gatgtccttc
     1801 gggtcgaact cgaagtcgag ccacgagccg cggtacggga tgatgcgggc ggagaacagc
     1861 agcttgccgg acgagtgggt cttgcccttg tcgtgctcga agaacacgcc gggcgagcgg
     1921 tgcagctggc tgacgatgac acgctcggtg ccgttgacga tgaacgagcc gtagtcggtc
     1981 atgagcggca cttcgcccat gtagacctcc tgctccttga tctccttgac gaccttggcc
     2041 ggcgcggact cgcggtcgta gatgatcatc tggagcttcg cgcgcacggc ggcggcgaac
     2101 gtgaggccgc gctgctggca ctcgcgcacg tcgaacggcg gcttcgcgat gttgtactcg
     2161 atgaacttca tctc
//
LOCUS       DATCRO010000955         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1090677, whole genome shotgun sequence.
ACCESSION   DATCRO010000955 DATCRO010000000
VERSION     DATCRO010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090677"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VIN75_26120"
     CDS             complement(<1..658)
                     /locus_tag="VIN75_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977718.1"
                     /translation="MKDLFENPMGLQGFEFVEFASPTAGVIEPYFEQLGFTLVAKHRS
                     KDVLLYRQGDINFIVNREPRSQAAFFAAEHGPSACGMAFRVKDSHKAYNRALELGAQP
                     IEIPTGPMELRLPAIKGIGGMPIYLIDRFEDGKSIYDIDFEFIDGVERRPAGHGLKLI
                     DHLTHNVYRGRMSFWADFYERLFNFREIRYFDIQGEYTGLTSKALTAPDGMIRIPLNE
                     E"
     gene            768..1268
                     /locus_tag="VIN75_26125"
     CDS             768..1268
                     /locus_tag="VIN75_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8977719.1"
                     /translation="MDTRFDLDTVDLRILRVLQANGRATYDEVAAAVSLSPSATLRRV
                     KRLEEAGVIAGYVAIVQPEAIGLALTAYITVRLEKQSDTHMRKPMDAFRASVQTWPDV
                     VECSSLTGEMDYLLRVLVSDMAHYSRFVMDTLLQHPSVQDCKTSFVLDRVKQTTAVPL
                     PESRSR"
     gene            1330..2094
                     /locus_tag="VIN75_26130"
     CDS             1330..2094
                     /locus_tag="VIN75_26130"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8977720.1"
                     /translation="MEQRFAGRTVIVTGAASGIGRGVAERFAWEGANVVMCDVEGDKV
                     RQAAAELPGDRTLALECDVSEAREVDKLVAAAVERFGALHVMVNNAGVAPEGSVTEVT
                     LHEWEDTLATDLSGVFHGCRSALPELLKTGGCIVNTASVSGLGADWRLAAYNAAKGGV
                     VNLTRSIALDYGPKGVRANAVCPSLTRTGMTEDMLEDQQLVDKFMERLPLRRVAEPAD
                     IAAVVAFLASDDARFVNGVNLPVDGGVMASNGQPRM"
     gene            complement(2120..>2326)
                     /locus_tag="VIN75_26135"
     CDS             complement(2120..>2326)
                     /locus_tag="VIN75_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8977721.1"
                     /translation="MWARLLKRHPAGKVAPFSLLVPVVGLYAAWIVFDERLGLLQWLG
                     VATVALGLLINNFGPRLRARLKAR"
BASE COUNT          374 a          768 c          831 g          353 t
ORIGIN      
        1 attcctcgtt cagcgggatg cggatcatgc cgtccggcgc cgtcagcgcc ttggacgtga
       61 ggcccgtgta ctcgccctgg atgtcgaagt agcggatctc gcggaagttg aacaggcgct
      121 cgtagaagtc ggcccagaac gacatgcggc cgcggtagac gttgtgcgtc aggtggtcga
      181 tcagcttcag gccgtggccg gcgggacggc gttcgacgcc gtcgatgaac tcgaagtcga
      241 tgtcgtagat ggacttgccg tcctcgaagc ggtcgatcag gtagatcggc atgccgccga
      301 tgcccttgat ggccggcagg cgcagctcca tcgggccggt ggggatctcg atgggctggg
      361 cgccgagctc cagcgcgcgg ttgtaggcct tgtgcgagtc cttcacgcga aaggccatgc
      421 cgcacgccga cgggccgtgc tcggccgcga agaatgcggc ctggctgcgc ggctcgcggt
      481 tgacgatgaa gttgatgtcc ccctggcggt acagcagaac gtccttggag cggtgcttgg
      541 cgacgagcgt gaagcccagc tgctcgaagt agggctcgat gacgcccgcg gtgggcgagg
      601 cgaactcgac gaattcgaag ccctgcaggc ccatcgggtt ctcgaacaga tctttcatgg
      661 cggtctccgg cgcgtgatcc gggaatcata gggaaatcgc ctcgcagcat ttctgcaaat
      721 ccgcgtccac ggtggcgatt tgtgcaggaa tcctgcatta tcctcaaatg gacacacgat
      781 tcgatctcga taccgtcgac ctgcgcatcc tgcgcgtgtt gcaggccaac ggccgcgcca
      841 cctacgacga ggtcgccgcg gcggtgagcc tgtcgccgtc cgcgacgctg cggcgcgtca
      901 agcggctcga ggaggccggc gtcatcgccg gctacgtggc gatcgtgcag cccgaggcca
      961 tcggcctggc actcaccgcc tacatcacgg tgcggctcga gaagcagtcg gacacgcaca
     1021 tgcgcaagcc gatggacgcg ttccgcgcgt cggtgcagac ctggcccgac gtcgtcgagt
     1081 gcagctcgct caccggcgag atggactacc tgctgcgcgt gctggtgagc gacatggccc
     1141 actactcgcg cttcgtgatg gacacgctgc tccagcatcc gagcgtgcag gactgcaaga
     1201 cgagcttcgt gctcgatcgc gtcaagcaga ccaccgcggt gccgctgccc gagtcgcgca
     1261 gccgctgagc gctgccgcgg cacaaggctt gccgccgccg gagcacatcc aacgattcgg
     1321 gggtatgcga tggagcagcg gttcgcgggc aggacggtca tcgtcacggg cgcggcgtcg
     1381 ggcatcgggc gcggcgtcgc ggagcgcttc gcctgggagg gcgccaacgt cgtgatgtgc
     1441 gacgtcgagg gcgacaaggt caggcaggcc gccgccgagc tgcccggcga tcgcacgctg
     1501 gcgctcgagt gcgacgtgag cgaggcgcgg gaggtcgaca agctggtcgc cgcggccgtc
     1561 gagcgcttcg gcgcgctgca cgtgatggtc aacaacgccg gcgtcgcgcc cgagggcagc
     1621 gtcacggagg tcacgctgca cgaatgggag gacacgctgg ccaccgacct gtcgggcgtg
     1681 ttccacggct gccgctcggc gctgcccgag ctgctcaaga caggcggctg catcgtcaac
     1741 acggcgtcgg tgtcgggcct gggcgcggac tggcggctgg cggcctacaa cgcggccaag
     1801 ggcggcgtcg tcaacctgac gcgctcgatc gcgctcgact acggccccaa gggcgtgcgc
     1861 gccaacgccg tgtgcccgtc gctgacgcgc accggcatga ccgaggacat gctcgaggac
     1921 cagcaactgg tcgacaagtt catggaacgc ctgccgctgc gccgcgtggc cgaaccggcc
     1981 gacatcgcgg ccgtggtggc cttcctcgcg agcgacgacg cgcgcttcgt caacggcgtg
     2041 aacctgccgg tcgacggcgg cgtgatggcc tcgaacgggc agccgcgcat gtgagcgcgg
     2101 ccgggccgcg cgtcgcgcgt cagcgcgcct tcagccgcgc gcgcaggcgc ggcccgaagt
     2161 tgttgatcag cagcccgagc gcgaccgtcg cgacgcccag ccactgcagc agcccgagcc
     2221 gctcgtcgaa gacgatccac gcggcataca gcccgacgac cggcacgagc agcgagaacg
     2281 gcgcgacctt gccggctgga tggcgcttca gcaggcgcgc ccacat
//
LOCUS       DATCRO010000956         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_506002, whole genome shotgun sequence.
ACCESSION   DATCRO010000956 DATCRO010000000
VERSION     DATCRO010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506002"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VIN75_26140"
     CDS             complement(<1..310)
                     /locus_tag="VIN75_26140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977722.1"
                     /translation="MSEQVAHAVPADLSTLAWVADELRRTLDNAHKALRRQVREVETA
                     PQGTEIDGSAAPAMQQARALFHQGAGALDMVGQAEGARMLRASEQAVLRYMGKIRTLD
                     N"
     gene            complement(454..2562)
                     /locus_tag="VIN75_26145"
     CDS             complement(454..2562)
                     /locus_tag="VIN75_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8977723.1"
                     /translation="MLDEGQPEEEVAAVDDDDTGLPLIGRRSIGYQQRFVITLAAIGF
                     AGLLGGGYLAITGANAHSKQVEATGAALMQSQRLAKSVSQAMIGSPAAFPEVKESGTV
                     LATDVRGLHAGNDVVTAVPGAAQDTVQPLLTLVDRAEKNAGIVLAQQKTLTQVGQALR
                     TINRQSSDLLETAETVSSLKLQGGASAAEISAVGQLVMLTQRIGKSANEFLTYEGVSP
                     EAVFLLGKDLNTFREIADGLLQGNADLRLPGTKEPATRERLEQLIQQYEQTRSQSSAI
                     LANLQGLVAAREAQAQIVGDSEPLRKGLEAVQSRLDQIGGVSIGYIVAIVIAALLLLL
                     GALGILRLFVVAQRQTAKEADEKRKEADARQREAQRVNDANQAAILRLMNELQSVAEG
                     DLTQQATVTEDITGAIADSVNYTVEELRTLVAQVQGTANRVTDTTGQVEQTSTELLAA
                     STEQLHEIRETGESVLQMAGRINDVSQRAQETVHAAQQSLTVAQAGRRAVQDAIEGMN
                     SIRDQIQETSKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGDAGRGF
                     SVVAEEVQRLAERSGDATRQIAALVKAIQTDTGDAVAAMERSTQGVVQGAQLSDAAGA
                     ALIDIDRVTRELSALVESISTQAQSEAQSANVVASNIQHIFAVTEQTGDGTRSTAQIV
                     RELSRTAEELRQSVSRFKIG"
BASE COUNT          399 a         1025 c         1065 g          552 t
ORIGIN      
        1 cgttgtccag cgtgcggatc ttgcccatgt agcgcagcac cgcctgctcg ctggcgcgca
       61 gcatgcgcgc gccttcggcc tgcccgacca tgtcgagcgc gcccgcgccc tggtggaaca
      121 gcgcgcgggc ctgctgcatc gccggggcgg cgctgccgtc gatctcggtg ccttgcggcg
      181 cggtctcgac ctcgcgcacc tggcgccgca gggccttgtg cgcgttgtcg agggtacggc
      241 gaagctcgtc ggccacccag gccaacgtgc tcaggtctgc aggcacggcg tgcgctactt
      301 gttcactcaa cgtcatctcc agcgccgcgc tcgtcacagc gcgcgcaagg gcggtcaacc
      361 atcaacgaga cagcctaccg ccgaaacgga tccagccggg catcgcatcc gaccggcggg
      421 acggatacgg cgaccagcgg gagcgtcggg gcatcagccg atcttgaacc gggacaccga
      481 ctggcgcagt tcttcagcgg tacgggacag ttctcgcacg atctgcgcgg tggaacgcgt
      541 accgtcgccg gtctgctccg tcaccgcgaa gatgtgctgg atgttcgagg ccacgacgtt
      601 cgcggactgc gcttcgctct gcgcctgcgt cgagatgctc tcgacgagtg cggacagttc
      661 gcgcgtcaca cggtcaatgt cgatcagcgc ggcgccggca gcgtcggaca gctgcgctcc
      721 ctgcaccacg ccctgggtcg agcgttccat ggcggccacg gcatcgccgg tgtcggtctg
      781 aatggccttc acgagcgccg cgatctgcct ggtcgcgtcg ccggagcgtt cggccagtcg
      841 ctgcacttct tccgcaacga cggagaagcc gcggcccgcg tcgccggcgg aggccgcctg
      901 gatggcggcg ttcagggcca gcacgttcgt ctgttccgta atgtcggaaa tcagctcggt
      961 gatttcgccg atctcctgcg acgattcgcc caggcgcttg atccgcttgg aggtttcctg
     1021 gatctggtcg cggatcgagt tcatgccctc gatggcgtcc tgcaccgcgc ggcgtccggc
     1081 ctgcgccacc gtcagcgatt gctgggcggc gtgcacggtt tcctgcgctc gctgcgacac
     1141 gtcgttgatt cggccggcca tctggagcac ggactcgccg gtctcgcgga tctcgtgcag
     1201 ctgctcggtg gaggccgcca gcagctcggt cgacgtctgc tcgacctggc cggtggtgtc
     1261 cgtcacccgg ttggcggtgc cctgcacctg cgccaccagg gttcgcagct cttccacggt
     1321 gtagttcacc gagtcggcga tggcgcccgt gatgtcctcg gtcacggtgg cctgctgcgt
     1381 caagtcgcct tcggcgaccg actgcagttc gttcatcagg cggagaatgg cggcctggtt
     1441 ggcgtcattg acgcgctggg cctcgcgctg gcgggcgtcg gcttccttgc gcttctcgtc
     1501 ggcttccttg gcggtctggc gctgggcgac cacgaacagt cgcaggatac cgagggcgcc
     1561 gagcagcagc agcagggccg cgatcacgat ggccacgatg tagccgatcg acacgccgcc
     1621 gatctggtcg agacggctct ggacggcttc caggcctttg cgcagcggtt cgctgtcgcc
     1681 cacgatctgc gcctgcgcct cacgcgcggc gaccaggccc tgcaggttgg ccagaatcgc
     1741 gctggactgg ctgcgggtct gctcgtactg ctggatcagc tgctcgaggc gctcgcgcgt
     1801 ggcgggctcc ttcgtgccgg gcagccgcag gtcggcgttg ccctgcagca ggccgtcggc
     1861 gatctcgcgg aaggtgttca agtccttgcc gagcaggaac acggcttcgg gcgacacgcc
     1921 ttcgtaggtc aggaattcgt tcgcgctctt gccgatgcgc tgcgtcaaca tgacgagctg
     1981 gccgacggcg gagatctcgg ccgccgaggc gccgccctgc agcttcagcg acgacacggt
     2041 ttccgcggtc tcgagcaggt cggacgactg ccgattgatg gtgcgcagcg cctggccgac
     2101 ctgggtcagc gtcttctgct gcgccagcac gatgccggcg ttcttctcgg cgcggtcgac
     2161 cagcgtcagc aacggctgca ccgtgtcctg ggcggcgccg ggcaccgcgg tcacgacgtc
     2221 gttgccggcg tgcaggccgc gcacgtcggt ggccagcacc gtgccagatt ccttcacttc
     2281 agggaaggcc gcggggctgc cgatcatggc ctgcgagacc gacttggcca ggcgctgcga
     2341 ctgcatcagc gccgcaccgg tggcttccac ctgcttggag tgcgcgttgg ccccggtgat
     2401 ggcgagatag ccgccgccga gcaggccggc gaagccgatg gccgccagcg tgatcacgaa
     2461 gcgctgctgg tagccgatgg agcggcggcc gatcagcggc aggccggtgt cgtcgtcgtc
     2521 gaccgcggcg acttcctcct cgggctggcc ttcgtcgagc atgcgcgtgg gcgcgggctc
     2581 ggcctggtcg ggcaccagcg tggagttggc gtgcgcggtg tcgacgcctt ccagcgggag
     2641 gtcgatcgac aggttgttct cggagtggac gacgtggtcg tcgtcctggc cggggccggc
     2701 accgtccagc gggaaggccg agtggcccat cgcgtggacg tccaggtcca gcgtcggcac
     2761 gccgccgccg gtgcccagcg cgtgcgtctt gtgctcggcg tgggtgccgt cggcggaatc
     2821 gtgcatcgcg tgcgcgtgcg aatcgccctg atcgctcagg tcgacgtccg ccagcgccac
     2881 gtcacggccg tgctcggaag cgacgggagg atagacgtta tcgacgctgc cctgggtatg
     2941 agcgccgtgt tgcttgagcg cgttaccgct gctttggtct gacaagttca tctcgccctc
     3001 ttcacgctag atttcaaatc ccaactaatc ccgaacggct g
//
LOCUS       DATCRO010000957        12021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_910629, whole genome shotgun sequence.
ACCESSION   DATCRO010000957 DATCRO010000000
VERSION     DATCRO010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910629"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VIN75_26150"
     CDS             complement(<1..729)
                     /locus_tag="VIN75_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8977724.1"
                     /translation="MPGTVMSYGTLLLHLDGTAQVPGLVDAAIQLARSHGSHVVGLAT
                     TGNTAFESSLGAGLLSRRDLLGAVEAARALAQQRAHDFDTVAAGAGLRSHEVVVDDRD
                     ASEALAARSLYCDLVVVGQPDAARPNPTWPASEFERALRGCASPILVLPAAARGAASF
                     GRRILVAWEAERACAHAITAALPLLRRAEQVQVVHVESPFSETLAEPPEDELERVRDR
                     LARDGVAASAWRRVTSGDLLELLMQ"
     gene            complement(750..1592)
                     /locus_tag="VIN75_26155"
     CDS             complement(750..1592)
                     /locus_tag="VIN75_26155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8977725.1"
                     /translation="MSYRTVLYSIEEDPIAESHIDYAANFARMFEARLVGVGCHRPSP
                     YPSDGAVAFIEGDPLTIELREAEEAAVAREAVFLQRCRLAGLTAVEAIRDNSTPTWAV
                     VGNALFADLVVTPQPSPALKVYAEHRRRAEAILQQSPRPVLMLPYAGHFVPQPETVVV
                     AWDGSPGAGRAASVALPMLRRASVVHLLQVFDPDKEDGSVLEPGLRRAAAWLGGHGIQ
                     VRTSLSANSLPVGQALLSEGADRGAQLFVMGAWGHGRLVERMLGGATRTLVESMTAPV
                     LFSH"
     gene            1770..3149
                     /locus_tag="VIN75_26160"
     CDS             1770..3149
                     /locus_tag="VIN75_26160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8977726.1"
                     /translation="MQLTFLGATGTVTGSRYLLETGRRRVLVDCGLFQGLKQLRLRNW
                     APLPVDPASIDAVVLTHAHIDHSGYLPRLVKQGFRGAIHATTATTELCALLLPDSGHL
                     QEEEALFANRHKTSRHDPALPLYTEEEARAALTRFHSHEFGAPFEPVPGLHMHFHSAG
                     HILGAASVQATSERGSVLFSGDLGRSDDLLMKPPAPPPAADMVVVESTYGNRLHERGD
                     TLLALAEVVNRTLARGGHVVVPAFAVGRAQLLLHALRLLKDAHRIPDVPVFLDSPMAT
                     DATTLYQRHPELHRLDANGCAAMCEGVRIVNSPDESRALASLRYPAIIVSASGMATGG
                     RVVHHIENFAPDPANTILFVGYQAMGTRGATLVAGAREVRMHGRWVPVRAEVATLEGL
                     SSHADRDELLAWLGALPHAPSRIFVTHGEPEAADSLRQAVTEHLHWPCEVPEYRQQVD
                     VGALADMVR"
     gene            complement(3151..3786)
                     /locus_tag="VIN75_26165"
     CDS             complement(3151..3786)
                     /locus_tag="VIN75_26165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8977727.1"
                     /translation="MRHLFSIASLAGAVLVAGCADPSRSRALDDPKVPGRALAEQVCS
                     NCHGQRGVASSENFPHLAAQQADYIDGQLRSLRDKHRRDPAGFVYMWGIASGLTDAQI
                     GQLAAYYAAQPAPAPDPAALAEPNAGKRLFTEGQAERGVPACAGCHGDRGQGNGAIPR
                     LAHQHRAYLLRQLAVLRQSPDNRPMGTLMNSVAHQLNDDDMRELADYISTM"
     gene            complement(3799..5286)
                     /locus_tag="VIN75_26170"
     CDS             complement(3799..5286)
                     /locus_tag="VIN75_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY8977728.1"
                     /translation="MPDLIARWMTRLVGGPRRRAAWLLAAMIATLAAPASAIPVFNRQ
                     TGQNCQACHAGGQYPELTPYGRMFKLTGYTIGGRTEIPLAMMAVASVSSVRNTGKSDD
                     PAADFSKNDKLVLASASVFAGGRITDNFGVFLQYTYDPYANLDASGKYHGHAAADNMD
                     IRYADRFVGTSHDLVIGASVNNNPSVADPWNTAAAWMQYVPVPSPSSSQFIDGASPYP
                     GYAVGGNTSGLTGYAFLDERLYLEAGLYRSTRGVLGFMKLGQPTPGLTRLQGNAPYLR
                     AAWNLAWGASTLMLGGSTMTSRVYDDPAVADPTTLHRYRDSTIDAQYQWLLDPHALTL
                     QAVASRQLHRYPDFLAGQVPAFVDAQGNALAATSTSDVLRLLRTKASYTWRATYGGSV
                     SYFDLSGTTNTLNQSSGYDPLTGTITSSSSAAAPSTRVNGNLTGNPRTTGQTLEAYWL
                     PWQNLRIGAQYTFYQRYNGSARNYDGFGRNASDNDSLFVYAWLAY"
     gene            complement(5452..5880)
                     /locus_tag="VIN75_26175"
     CDS             complement(5452..5880)
                     /locus_tag="VIN75_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8977729.1"
                     /translation="MKRTVSSMMSRDVCVVDMDDTLASVEQRLADRRLSWAPVLEGSA
                     GTVIGVIGAADLLRLRAQGGDSQAVRAWQMCAFKPLTVSADADLFEVAREMVERHLHH
                     VVVTEGGAVVGVLSSLDFVREVASRGARATSEPPDKTPHP"
     gene            complement(5877..8327)
                     /gene="ppsA"
                     /locus_tag="VIN75_26180"
     CDS             complement(5877..8327)
                     /gene="ppsA"
                     /locus_tag="VIN75_26180"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY8977730.1"
                     /translation="MSTSTRYIRWFNELGTADVALVGGKNASLGEMLSNLAPAGVKVP
                     NGFAITAQAYRHALDQADAWPRLHRALDGLDPSDIDDLARRAQAAREIVAAAPLPPDL
                     VAEIRDAYRALLDEYGGELTVAVRSSATAEDLPDASFAGQHETFLNVAGEARLLESVR
                     QCFASLFKDRAIAYRVEKGFDHFKVLQSVGVMKMVRSDRASSGVVFTLDTESGFRDAV
                     FITGAYGLGENVVQGTVDPDEFYVFKPTLRRGHRAVLRRKLGGKEIRMVYAQAAGRET
                     TRNVPTPREEQERFCIGDDDVLALADAAIAIEDHYTRRLGRPTPMDIEWARDGIDGQI
                     YIVQARPETVASQRPFGVIEESRLDAKGVLRVSGRAVGGAIATGRARVITDVAQLAEF
                     RPGEILVADTTMPDWGTVMKIAAAVVTNRGGRTCHAAIVARELGIPAVVGCDAATRSI
                     RTGDELTVSCGEGATGHVYEGILPFTKSRTDISTLDLPQTQIMVNLGNPDVAFQTASL
                     PVDGVGLARMEFIVAEHIRVHPMALVHPERVQDPAVRAEIARLTHGHATPADHFVRQL
                     AEGVGTIAAAFYPGPVIVRMSDFKTNEYASLLGGRDFEPVEANPMLGFRGASRYGHPA
                     YAEGFALECAAMKRVREDMGLTNVKLMIPFCRRVEEGQRVIAAMEALGLKRGIGGLEI
                     YVMCEIPNNVVQIDAFAKLFDGFSIGSNDLTQLVLGVDRDSETVAFDFDERDPGVQAL
                     IRQTVDGAHRNGRHVGICGQAPSDYPEMVEFLVGLGIDALSVTPDVALKVLGNVLAIE
                     RRLGRPSRAQSDRKEGTS"
     gene            complement(8324..9529)
                     /gene="ugpC"
                     /locus_tag="VIN75_26185"
     CDS             complement(8324..9529)
                     /gene="ugpC"
                     /locus_tag="VIN75_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8977731.1"
                     /translation="MAAISLRRLSKRFGTTSVLKDVDLDIADGEFIAMVGPSGCGKST
                     LLNCIAGLEAPTDGEVWIGGRDVTWLAPKDRDIAMVFQAYALYPNMTVRQNILFGLEI
                     RNEPKDRRDAALKRVSEMLRIGDLLERKPAQLSGGQRQRVAMGRAMVRDPAVFLFDEP
                     LSNLDAKLRVQMRAELKALHQRLHQTVVYVTHDQVEAMTLADRIVVMNGGRLDQVGTP
                     TELYDRPVDLFVAGFIGSPAMNFIVGRTALRDPGPVLVCDDGHEFPLPADLPISAGRA
                     LTVGVRPEHLGVAADGPVRLKVDIVEPTGDETNLYGKVGDELVCVRLHHRAAIAPGQL
                     VALAWPLDRLHLFDSASGRRIDAPPLESAEAPVAEVQPPPPVKAGGATTAARRPDFLK
                     FLRPRRPTT"
     gene            complement(9729..10214)
                     /locus_tag="VIN75_26190"
     CDS             complement(9729..10214)
                     /locus_tag="VIN75_26190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8977732.1"
                     /translation="MYSKILVPVDGSEPSQCGLREAIGIAREHKSTLLLLHVIVQMPL
                     TLGYPGVADYGQVWDMLDRAGREIVERAAKEAAAAGVAYVTRVVDGGAGAVCDVVVDQ
                     ADSEHCELIVMGTHGRRGMKRLALGSDAELIVRHSPVPVLLVKAPPAANVPKAGARAT
                     A"
     gene            10613..11113
                     /locus_tag="VIN75_26195"
     CDS             10613..11113
                     /locus_tag="VIN75_26195"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEY8977733.1"
                     /translation="MSESDPRSVRRRGPHHTGFDQPDAKDPCRARRKTAEPAACPDCG
                     AVQEAGQWRWRRCSPGARPLRCPACCRIRDNLPAGELHLTGDFLAAHRDEVLARVRHV
                     VEHTRKRYVLQRLMEIDEDDRQVRITTTSPHLARAIGLALHDAFKGTLDLDMQTRGCP
                     RVRWTR"
     gene            complement(11134..11613)
                     /locus_tag="VIN75_26200"
     CDS             complement(11134..11613)
                     /locus_tag="VIN75_26200"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8977734.1"
                     /translation="MTTRRSVAFVASLAALLGACAVRLDNQRPAEQLARESAPPGSAY
                     DGWRLYNQRCAGCHGKDAAGAAPMPDLLDRVSQMGPRRFANVVLTRYDWSETVAEDEG
                     TAPIPRLYLRKTPLGGSQVLEMPAWANDPAVVGHVGDLYAYLAGRADGRLATGSPPR"
     gene            11788..>12021
                     /locus_tag="VIN75_26205"
     CDS             11788..>12021
                     /locus_tag="VIN75_26205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEY8977735.1"
                     /translation="MADVLVVFHSRSGHCRMVAEALSHKRGWSLGEVVYLQGPQSYGR
                     CARDALLRREPEIRYKGANPSGFEVVVMVSPVWC"
BASE COUNT         1801 a         4247 c         4168 g         1805 t
ORIGIN      
        1 ctgcatcagg agctcgagca gatcgccgct cgtgacgcga cgccaggcgc tggccgcgac
       61 gccgtcgcgc gccagccgat ccctgacgcg ctccagctcg tcctccggcg gctcggcgag
      121 cgtctccgag aacggcgatt cgacatgcac gacctgcacc tgctccgccc ggcgcagcag
      181 gggcagcgcc gccgtgatcg catgcgcgca cgcccgctcc gcctcccagg ccacgaggat
      241 gcgccggccg aacgacgccg cgcccctggc cgctgcgggc agcacgagga tcggcgacgc
      301 gcacccgcgc agggcgcgct cgaactcgga ggccggccac gtcgggttgg gtcgcgccgc
      361 gtcgggttga ccgacgacga cgaggtcgca gtacaggctg cgtgccgcga gcgcctccga
      421 ggcgtcccgg tcgtcgacga ccacttcgtg cgagcgcagg cccgcgcctg ccgcgacggt
      481 gtcgaaatca tgcgcgcgct gctgcgcgag tgcgcgcgcc gcctcgacgg cgccgagcag
      541 gtcgcgccgg gacagcaggc ccgcgcccag gctgctctcg aacgcggtgt tgcccgtggt
      601 ggcgagtccg accacgtggc tcccgtgcga ccgcgcgagc tggatcgccg catcgacgag
      661 gccgggcacc tgcgccgtgc cgtccaggtg cagcaacaac gttccatagc tcatcacggt
      721 ccccggcatt ggatggccct cccatgcgcc tagtgagaga acagaaccgg cgcggtcatg
      781 ctttcgacca gggtgcgcgt cgcaccgccc agcatccgct cgacgaggcg gccgtgtccc
      841 catgcgccca tcacgaacag ctgcgcgccc cgatccgcgc cctcggacag cagcgcctgt
      901 ccgacgggca gcgagttggc ggacagcgac gtcctgacct ggatgccatg cccacccagc
      961 cacgccgcgg cgcgccggag gccgggctcc agcaccgagc cgtcttcctt gtcgggatcg
     1021 aagacctgga gcaggtgcac gacgctcgcc cgccgcagca tcggcaacgc gacggacgcc
     1081 gccctgcccg caccggggct cccgtcccac gcgacgacca ccgtctcggg ctgcggtacg
     1141 aaatggcctg cgtaggggag catcagcacc ggccgggggc tctgctgcag gatggcttca
     1201 gcgcgacgcc ggtgctcggc gtagaccttg agcgcgggcg aaggctgcgg cgtcacgacc
     1261 aggtcggcga acagcgcgtt gccgaccacc gcccaggtcg gcgtggagtt gtcgcggatt
     1321 gcctcgaccg cggtcagccc ggccaggcgg catcgctgca ggaagaccgc ttccctggcc
     1381 acggccgcct cctccgcctc gcgcagctcg atcgtgagcg gatcaccctc gatgaaggcg
     1441 accgcgccgt cgctcggata gggtgacggt cggtggcagc cgacgccgac gaggcgtgcc
     1501 tcgaacatgc gggcgaaatt cgccgcgtag tcgatgtgcg attccgcgat cgggtcttcc
     1561 tcgatcgaat agaggacggt gcgatagctc atgggatttc ctcatgcagc cccgtcgacg
     1621 atggcagggc gctcgaggga atcgtcggct cgcttccgca gcggccgctt gcgcggaatc
     1681 aactcgccca tcggtcgcgg ctgatgcgca tcaatcggac ggaggcgccg cggcctatcg
     1741 tgcgtgcagc caaacatcgg ggattcgcca tgcagctcac cttcctgggt gccaccggca
     1801 ccgtcaccgg ctcgcgctac ctcctggaga cgggccgccg ccgcgtgctg gtggactgcg
     1861 gcctgttcca gggcctgaag cagctgcgcc tgcgcaactg ggcgccgctg cccgtcgacc
     1921 ccgcatccat cgacgccgtc gtcctcaccc acgcgcacat cgaccattcc ggctacctgc
     1981 ctcgcctcgt caagcaaggc tttcgcggcg cgatccatgc caccacggcg acgaccgagc
     2041 tctgcgccct gctgctgccc gactccggac acctgcagga ggaggaggcc ctgttcgcca
     2101 accggcacaa gacgagccgc catgatccgg cgctgcccct gtacacagaa gaggaggcgc
     2161 gtgcggcgct gacgcggttc cattcacacg aattcggcgc gccgttcgaa ccggtgcccg
     2221 gcctgcacat gcacttccac agcgccgggc acatcctggg cgccgccagc gtccaggcca
     2281 ccagcgagcg cggcagcgtc ctgttctccg gcgatctcgg ccgcagtgac gacctgctga
     2341 tgaagccgcc ggcgccgccg ccggcagccg acatggtcgt ggtcgaatcg acctacggca
     2401 accgcctgca cgagcgcggc gacacgctcc tcgcgctcgc cgaagtggtc aaccggaccc
     2461 tggcacgcgg cggccacgtg gtcgttccgg ccttcgccgt cggccgcgcc cagctgctgc
     2521 tgcatgcctt gcgattgctc aaggacgccc accggatccc ggacgtcccg gtcttcctcg
     2581 acagtccgat ggccacggac gccaccaccc tgtaccagcg ccaccccgag ctgcaccggc
     2641 tcgacgcgaa cggctgcgcc gcgatgtgcg agggcgtgcg catcgtcaac tcgcccgacg
     2701 agtcgcgcgc gctcgcatct ctgcgttacc cggccatcat cgtgtcggcc agcggcatgg
     2761 ccacgggtgg ccgcgtcgtc caccacatcg agaacttcgc gcccgatccg gccaacacga
     2821 tcctgttcgt cggctaccag gcgatgggca cgcgcggcgc gacgctcgtc gccggcgcgc
     2881 gcgaggtgcg gatgcacggc cgctgggtgc cggtgcgcgc ggaggtggcg acgctggaag
     2941 gcctgtcgtc gcacgccgac cgcgacgagc tgctcgcctg gctgggcgcc ctgccccatg
     3001 cgccgagtcg catcttcgtc acccacggcg agccggaggc ggcggactcg ctgcgccagg
     3061 ccgtcaccga gcacctgcat tggccgtgcg aggtgccgga atatcgccag caggtcgatg
     3121 tcggcgcgct ggccgacatg gtccgctaga tcacatcgtc gagatgtagt ccgccaactc
     3181 gcgcatgtcg tcgtcgttca gctggtgcgc cacggagttc atcagcgtgc ccatgggacg
     3241 gttgtccggg ctctggcgca ggacagccaa ttggcgcagc aggtaggcgc gatgctgatg
     3301 cgcgagccgg gggatggcgc cgttgccctg gcccctgtcg ccgtggcaac cggcgcaggc
     3361 cggcacgccg cgttctgcct ggccctcggt gaacagccgc ttgccggcgt tcggctcggc
     3421 aagcgcggcg gggtccggtg ccggcgccgg ctgcgcggcg tagtaggcgg ccagctgccc
     3481 gatctgggcg tcggtcaggc cgcttgcgat gccccacatg tagacgaaac cggccgggtc
     3541 gcggcggtgc ttgtcgcgca ggctgcgcag ctggccgtcg atgtagtcgg cctgttgcgc
     3601 ggccagatgg gggaagttct ccgacgacgc gacgccgcgc tgcccgtggc agttcgagca
     3661 gacctgctcg gccagcgccc gtccgggaac cttgggatcg tccagcgcac gggagcgcga
     3721 cggatcggca caacccgcca cgaggacggc gccggcgagc gaggcgatcg agaacaggtg
     3781 tcgcatcatg atctccactc agtaggcgag ccacgcgtac acgaacagcg agtcgttgtc
     3841 gctcgcgttg cgcccgaagc cgtcgtagtt gcgggcactg ccgttgtagc gctgatagaa
     3901 ggtgtactgc gcgccgatgc gcaggttctg ccaggggagc cagtacgcct cgagcgtctg
     3961 ccccgtcgtc ctcgggttgc ccgtcaggtt gccgttgacc cgcgtcgacg gcgccgcggc
     4021 cgatgacgac gacgtgatgg ttcccgtcag cgggtcgtag cccgacgact ggttcaaggt
     4081 gttcgtcgtg cccgacaggt cgaagtacga gacgctgccg ccataggtgg cgcgccacgt
     4141 gtacgacgcc ttcgtgcgca gcagcctgag gacatcggac gtcgaggtcg ccgccagcgc
     4201 attgccctgg gcgtcgacga aggccggcac ctgcccggcc aggaagtccg ggtaccgatg
     4261 gagctggcgc gaggccaccg cctgcagcgt caacgcgtgg ggatcgagca gccactggta
     4321 ctgcgcatcg atcgtcgaat cgcgatatcg atgcaaggtc gtcggatcgg cgaccgcagg
     4381 atcatcgtag acgcgcgacg tcatcgtcga tccgccaagc atcagcgtgc tcgcacccca
     4441 cgcgaggttc caggcggcac gcaggtacgg cgcgttgccc tgcaggcggg tcaggccggg
     4501 cgtcggctgg ccgagcttca tgaatcccag cacgccgcgt gtcgaccggt agaggcccgc
     4561 ctccaggtag aggcgctcgt cgaggaaggc gtacccggtg aggccggacg tgttgcctcc
     4621 cacggcgtag ccgggatagg ggctggcgcc gtcgatgaac tggctgctgg acggactggg
     4681 caccggcacg tactgcatcc aggccgccgc ggtgttccag ggatcggcga cggacgggtt
     4741 gttgttcacg ctggcgccga tcaccaggtc gtgcgacgtg ccgacgaagc ggtcggcgta
     4801 gcggatgtcc atgttgtcgg ccgccgcgtg cccgtgatac ttgcccgagg catcgaggtt
     4861 cgcgtacggg tcgtaggtgt actggaggaa cacgccgaag ttgtcggtga tccgcccgcc
     4921 cgcgaagacg ctggccgacg cgaggacgag cttgtcgttc ttcgagaagt cggcggccgg
     4981 atcgtcgctc ttgccggtgt tgcgcaccga cgagacgctg gcgaccgcca tcatcgccag
     5041 ggggatctcc gtgcggccgc cgatcgtgta gcccgtgagc ttgaacatcc ggccgtaggg
     5101 cgtcaactcc gggtactgcc ctccggcgtg gcaggcctgg cagttctgtc cggtctgccg
     5161 gttgaagacc gggatggcgg atgccggcgc ggccagcgtc gcgatcatgg ccgcgagcag
     5221 ccacgccgcg cggcgccgcg ggccgccgac cagtcgggtc atccatcgtg cgatcaggtc
     5281 gggcatggga acctccgtcg tgtctgggct ccattccagc gcgtcccgca gccgatgcgt
     5341 tgacgaggct caagcgtcga cccgcgcttg cttgcgcagc cctgaacacc gcgcgaagcc
     5401 ttgcaggcag cgattggcga tggtcatgct ggatccttcc tggtttgcat gtcatggatg
     5461 cggcgtcttg tcgggaggct cggatgtcgc gcgagcgccg cgcgaggcga cttcgcgcac
     5521 gaaatcgagc gacgacagca cgccgaccac ggcaccgccc tcggtgacca ccacgtgatg
     5581 gagatggcgt tcgaccatct cgcgtgcgac ctcgaacagg tcggcgtccg cgctcaccgt
     5641 caggggcttg aaggcgcaca tctgccaggc gcgaaccgcc tgcgaatcgc caccctgcgc
     5701 gcgcaggcgc agcaggtcgg ccgcgccgat cacgccgatc accgtgccgg cagacccttc
     5761 cagcaccgga gcccaggaca ggcgtcggtc ggcgagccgc tgctcgaccg aggcgagcgt
     5821 gtcgtccatg tcgacgacgc agacgtcgcg cgacatcatc gaagagaccg tgcgtttcat
     5881 gatgttcctt ccttgcgatc ggactgcgcg cgcgacgggc gcccgagccg gcgctcgatc
     5941 gccaggacgt tgcccagcac cttcagggcg acatccggcg tgacgctcag cgcgtcgatg
     6001 ccgagtccca ccaggaactc gaccatctcc gggtagtcgg acggcgcctg cccgcagatg
     6061 cccacatggc gtccgttgcg atgggcgcca tcgaccgtct gccggatgag cgcctggaca
     6121 ccgggatcgc gctcgtcgaa atcgaaggcg acggtctcgg aatcgcggtc gacccccagc
     6181 accagctgcg tgagatcgtt ggatccgatc gagaagccgt cgaacagctt ggcaaaggca
     6241 tcgatctgga ccacgttgtt cgggatctcg cacatcacgt agatctccag cccgccgatg
     6301 ccccgcttca ggcccagggc ctccatggcc gcgatcacgc gctgcccctc ctccacccgg
     6361 cggcagaacg ggatcatcag cttgacgttg gtcaggccca tgtcctcgcg gacgcgcttc
     6421 atcgccgcgc actcgagcgc gaacccttcc gcataggcgg gatggccata ccgcgacgcg
     6481 ccgcggaagc ccagcatcgg gttcgcctcg acgggctcga agtcgcggcc gcccagcagg
     6541 ctcgcgtatt cgttcgtctt gaaatccgac atgcgcacga tcaccggccc ggggtagaag
     6601 gcggccgcga tcgtgcccac gccctcggcg agttgccgga cgaaatggtc ggccggcgtc
     6661 gcatggccgt gcgtcagtcg cgcgatctcc gcacgcacgg ccggatcctg cacgcgctcc
     6721 ggatgcacga gcgccatggg gtgcacgcgg atgtgctcgg caacgatgaa ctccatgcgc
     6781 gccaggccga ccccgtccac cggcagcgaa gcggtctgga aggcgacgtc cggattgccg
     6841 aggttgacca tgatctgcgt ctgcggcagg tcgagcgtgg agatgtccgt gcggctcttc
     6901 gtgaacggga ggatgccctc gtagacatgt ccggtcgcgc cctcgccgca cgacaccgtc
     6961 agctcgtcgc ccgtgcgtat cgagcgcgtg gctgcgtcgc agccgacgac cgccgggatg
     7021 ccgagttccc gggcgacgat ggccgcatgg caggtgcgcc ccccgcggtt ggtcaccacg
     7081 gccgcggcga tcttcatgac cgtgccccag tcgggcatcg tggtgtcggc caccaggatc
     7141 tcgcccggcc tgaactcggc cagttgcgcg acgtcggtga tgacgcgtgc gcgccccgtc
     7201 gcgatggcgc cgcccaccgc gcgtccgctg acgcgcaaga cgcccttggc gtcgaggcgg
     7261 ctctcctcga tcacgccgaa gggacgctgc gacgccaccg tctccggccg ggcctgcacg
     7321 atgtagatct ggccgtcgat gccatcccgg gcccattcga tgtccatcgg cgtcggccgg
     7381 cccagtctgc gggtgtagtg gtcctcgatg gcgatcgcgg cgtcggccag ggccagcacg
     7441 tcgtcgtcgc cgatgcagaa gcgttcctgc tcctcgcgcg gcgtcggcac gttgcgggtg
     7501 gtctcgcggc cggcggcctg cgcatagacc atccggatct ccttgccgcc gagcttgcgc
     7561 cgcagcaccg cccgatggcc gcggcgcagc gtcggcttga acacatagaa ctcgtcggga
     7621 tccaccgtcc cctgcaccac gttctcgccg agcccgtagg ccccggtgat gaagacggca
     7681 tcgcggaaac cggactcggt gtccagcgtg aagaccaccc cgctcgacgc gcgatcggag
     7741 cgcaccatct tcatcacgcc gacggactgc agcaccttga agtggtcgaa gcccttctcg
     7801 acgcgatacg cgatggcgcg atccttgaac aggctcgcga agcactggcg caccgactcc
     7861 agcaggcgcg cctcgccggc gacgttgagg aaggtctcgt gctgtcctgc gaagctggcg
     7921 tcgggcaggt cctcggccgt ggccgaactc cggacggcga cggtcagctc gccgccatat
     7981 tcatcgagca aggcccggta ggcgtcacgg atctcggcga ccaggtcggg cggcagcggc
     8041 gccgccgcca cgatctcgcg cgcggcctgc gcgcgccggg cgaggtcgtc gatgtcggac
     8101 gggtcgaggc cgtcgagcgc gcgatgcagc cgcggccagg cgtccgcctg gtcgagcgcg
     8161 tgccggtagg cctgtgccgt gatcgcgaag ccgttcggca ccttgacgcc cgcgggggcc
     8221 aggttgctca gcatctcgcc cagggaggcg ttcttgccgc ccaccagggc gacgtccgcc
     8281 gtgcccagtt cgttgaacca gcggatgtat cgagtcgatg tgctcatgtc gtgggtctcc
     8341 ttggtcggag gaacttgagg aagtccgggc gccgtgcagc cgtcgtggcg ccgccggcct
     8401 tgaccggggg cggcggctgc acctcggcga ccggcgcttc ggcggactca agcggcggcg
     8461 cgtcgatgcg acgcccgctg gccgagtcga acaggtgcag gcgatcgagc ggccaggcca
     8521 gcgcgaccag ctgccccggg gcgatggcgg cgcgatggtg cagccgcacg cagacgagct
     8581 cgtcgccgac cttgccgtag aggttggtct cgtcgccggt cggttcgacg atgtcgacct
     8641 tcaggcggac cgggccgtcg gccgcgactc cgagatgctc cgggcgaacg ccgaccgtga
     8701 gcgccctgcc ggcggagatg ggcagatcgg cgggcaacgg aaactcgtgc ccgtcgtcgc
     8761 acacgagcac cgggccggga tcgcgcaagg ccgtgcgccc gacgatgaag ttcatggccg
     8821 gcgagccgat gaagccggcg acgaacaggt cgacgggacg gtcgtagagc tccgtcggcg
     8881 tgccgacctg gtcgaggcgg ccgccgttca tgacgacgat gcgatcggcc agggtcatgg
     8941 cctccacctg gtcgtgcgtg acgtagacga ccgtctggtg caggcgctgg tgcagggcct
     9001 tgagctcggc gcgcatctgc acgcgcagct tcgcgtcgag gttggacagc ggctcgtcga
     9061 acaggaagac cgccgggtcg cgcaccatcg cccgccccat cgcgacccgc tggcgctgcc
     9121 cgcccgacaa ctgtgccggc ttgcgctcca gcaggtcgcc gatgcgcagc atctccgaga
     9181 cgcgcttcaa cgcggcgtcg cgacgatcct tcggctcgtt gcggatctcc aggccgaaca
     9241 ggatgttctg gcgtaccgtc atgttcggat agagcgcgta ggcctggaac accatcgcga
     9301 tgtcgcggtc cttcggagcc agccaggtga cgtcgcggcc gccgatccag acctcgccgt
     9361 cggtgggcgc ctccagcccc gcgatgcagt tcagcagcgt ggacttgccg cagcccgacg
     9421 ggccgaccat ggcgatgaac tcgccgtcgg cgatgtccag atccacgtcc ttcagcacgg
     9481 acgtggttcc gaaacgcttg gacaacctgc gcaggctgat ggccgccatc acgatcctcg
     9541 ggagtacgct cgattcggcg gccgtcgcgg tccgggcaag ttgcgcaaga tcaagggcgg
     9601 gacgtcgcct cgcacgccgg gccgagatcc ctcgccggca cggcctgtgc acgacgcgtt
     9661 cgggaacgca cgaatgccgg gcgtcggcgt cgatctcgac gaggccgctt cgttgcgcat
     9721 cgggaacctc aggcggtcgc cctggccccg gctttcggga cgttcgccgc gggtggcgcc
     9781 ttcacgagca gcaccggcac gggcgaatgc cgcacgatca gctcggcgtc gctgcccagc
     9841 gccaggcgct tcatgccgcg ccgcccgtgc gtgcccatga cgatcagctc gcaatgctcc
     9901 gaatcggcct ggtcgaccac gacgtcgcag accgccccgg caccgccgtc gacgacccgc
     9961 gtgacgtagg ccacgcccgc cgccgcggcc tccttggccg cccgttcgac gatctcgcgt
    10021 ccggcccgat cgagcatgtc ccagacctgg ccgtagtccg cgacgcccgg atagccgagg
    10081 gtcagcggca tctgcacgat cacgtgaagc agcagcagcg tggacttgtg ttccctggcg
    10141 atgccgatgg cctcccgaag gccgcactgc gacggctcgc tgccgtcgac ggggacgagg
    10201 atcttcgaat acatgactgc gctccgatgc cgatccgcgc ggcacgacga tcgggtcgcg
    10261 cacggtcgaa ccatgatgtc ggtcccgggc cgccgtccct tgcgcccgcg caagccgccg
    10321 gtggcggcga ctcaatcgag cgacttggga aagcgcagca cggaggcggt cggcagcacc
    10381 gtcccgagca gcgccagcgc gatcagctcg ggccagagca cgtcagccgg gatgccctgg
    10441 gacgacgcgg cggcgcgatc tcaccggcct tgcgcggaaa agcggacgga attgagcccg
    10501 cgcaaccggg tgccggcgtc ggtcggttgc aatcgtctcg tcgctgtcgg gatcgacgcg
    10561 gtgcaaccgc accgtcggcg ccaagccgca gcaggcgtcc cgaggaagca agatgtccga
    10621 atccgacccg cggtcggtgc ggcggcgcgg gccgcaccac accggcttcg atcaacctga
    10681 cgcgaaggat ccctgccgcg cccgccgcaa gacggcggaa ccggccgcct gtcccgattg
    10741 cggtgccgtg caggaagccg gccaatggcg atggcgcagg tgctcgcctg gcgcgcggcc
    10801 gctgcgttgc ccggcctgct gccggatccg ggacaacctg ccggccggcg aattgcacct
    10861 gaccggtgac ttcctggccg cacaccggga cgaggtcctc gcgcgcgtgc ggcacgtggt
    10921 cgagcacacg cgcaagcgct acgtcctgca gcgcctcatg gagatcgacg aggatgaccg
    10981 gcaggtccgc atcaccacga ccagcccgca cctggcgcga gccatcggcc tggccttgca
    11041 tgacgcgttc aagggcaccc tcgacctcga catgcagaca cgaggctgtc cgcgcgtccg
    11101 ctggactcgc tgaagctgcg acgacgcgcg cagtcaccgc ggcgggctgc cggttgccag
    11161 ccgtccgtcg gcccggccgg cgaggtaggc gtagaggtca ccgacatggc ccacgacggc
    11221 ggggtcgttg gcccacgccg gcatctccag cacctgcgag ccgcccagcg gcgtcttgcg
    11281 caggtacagg cgcgggatgg gcgccgtgcc ctcgtcttcg gccaccgtct cgctccagtc
    11341 gtagcgcgtg agcacgacgt tggcgaaacg gcgcgggccc atctgggaga cgcgatccag
    11401 caagtcgggc atcggggcgg cgcctgccgc atccttgcca tggcagccgg cacagcgctg
    11461 gttgtaaagc ctccagccgt cgtaagcgga ccctggcggc gccgattcgc gcgccagttg
    11521 ctcggccggc ctctggttgt cgagccggac ggcgcaggcg cccagcagcg ctgcaagcga
    11581 tgcgacgaag gcgacggatc ggcgggtcgt catgatgtct cccgaagacg caacgcccca
    11641 ttgtggtaac gccgcagtcg tcgcgcattg attcggcgca tgccccgacg cgacctcgaa
    11701 agtcggaacg tccacgcatt gatgaggcgc aatcggcgct tgcggccggc tacgacactg
    11761 atgcaccaca tgtcgaagga gtgctccatg gccgacgtcc tggtcgtctt ccattcccgc
    11821 tcgggccatt gccggatggt cgccgaggcg ctgagccaca agcgtggctg gtcgctgggc
    11881 gaagtcgtct acctgcaggg ccctcagagc tatgggcgct gcgcccgcga tgcgttgctg
    11941 cgccgcgaac cggagatccg ctacaagggc gccaatccct ccggcttcga ggtggtggtg
    12001 atggtctcgc cggtgtggtg c
//
LOCUS       DATCRO010000958         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_258114, whole genome shotgun sequence.
ACCESSION   DATCRO010000958 DATCRO010000000
VERSION     DATCRO010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258114"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VIN75_26210"
     CDS             complement(<1..566)
                     /locus_tag="VIN75_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8977736.1"
                     /translation="MRRIAFAAFAAAASLAGCALHPVGPDYKLPDTAIVDRPGAAAPF
                     AHAGASAASGAAPFADLPLPPHWWRLYQDPKLDALVQKALAHNTDLRVALATLQKAEA
                     ADRAVAGAGRPTISAEGGPAFGHASGLTYLAPNYVPPSGWEYGASAALSYQLDLFGKL
                     QRAIEASAADTQAAQAALDLARVNVAAGT"
     gene            complement(569..1507)
                     /locus_tag="VIN75_26215"
     CDS             complement(569..1507)
                     /locus_tag="VIN75_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8977737.1"
                     /translation="MTSDLLAPGRWPMRLARLLVTLAVVLAAAWAASRLWDHYELEPW
                     TRDGRVRANVVQIAPDVSGLVTAVPVQDNQRVAAGALLFEVDRARYELALRQAQASLT
                     AAKVTLAQARRENARNVDLGNLISQELREQTATRVEAAQAGVAQAGVALDAARLNLER
                     AQVRAPADGLVTNLDMRSGSYAQAGRAALALVDARSFYVEGYFEETKLPRIHVGDRVR
                     VTPMGGARLDGTVESIAAGIADRDRSTGSNLLPSVNPTFNWVRLAQRIPVRIRLDALQ
                     PGTRLVAGETVTVQIVADTSATLAQRPPVAASATRS"
     gene            complement(1516..1728)
                     /locus_tag="VIN75_26220"
     CDS             complement(1516..1728)
                     /locus_tag="VIN75_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY8977738.1"
                     /translation="MIGEAAFHGLYVPWLMLLCGLTLAVQWLVHRLLGAIGFYRWVWH
                     PALFDAALYLLLLYVVSSAASLLQTH"
     gene            complement(1725..>2411)
                     /locus_tag="VIN75_26225"
     CDS             complement(1725..>2411)
                     /locus_tag="VIN75_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY8977739.1"
                     /translation="LLGLFCARPATAPSAMAMIFGVAGTLAMHDTSATVELPAFVNSL
                     LAQVLGIYAAALVTRLVRTVGADWTAQRIRRATWSELGAMADAPRGAPPDDAYAVRMV
                     DRIALLAPRVAQADPAVRNDTVEGALRDLRSGLDIVALQRARPRLPSMGIGAVLGGVA
                     RLFRLRGAGRDGAPPASLLADFDGALARALAGDGRDTRAAVTALVGLRRALFPAAPAA
                     LVTPSTGVLA"
BASE COUNT          334 a          858 c          947 g          272 t
ORIGIN      
        1 gtgccggccg ccacgttgac gcgcgcgagg tcgagggcgg cctgcgcggc ctgggtgtcg
       61 gcggccgagg cctcgatcgc gcgctgcagc ttgccgaaca ggtccagctg gtacgacagc
      121 gcggcgctcg caccatattc ccagccgctg ggcggcacgt agttcggggc gaggtacgtc
      181 aggcccgatg cgtggccgaa ggcggggccg ccctcggccg agatcgtcgg ccggcccgcg
      241 cccgcgacgg cgcggtcggc cgcctcggcc ttctgcagcg tggccagcgc gacgcgcagg
      301 tcggtgttgt gcgcgagcgc cttctggacc agcgcgtcca gcttcggatc ctggtacagc
      361 cgccaccaat gcggcggcag cggcaagtcg gcgaacgggg cggcgcccga ggcggcggac
      421 gcgccggcgt gggcgaacgg cgcagcggcg ccgggccggt cgacgatcgc ggtgtcgggc
      481 agcttgtagt cgggaccgac cggatgcagg gcgcagccgg cgagcgaggc ggcggccgcg
      541 aacgccgcga aggcgatgcg tcgcatgatc agctccgggt ggccgacgct gccaccggcg
      601 gacgctgcgc cagcgtcgcg gacgtgtcgg cgacgatctg caccgtcacc gtctcgcccg
      661 cgacgagacg cgtgccgggc tgcagcgcat cgagcctgat gcgcaccggg atgcgctgcg
      721 cgagccgcac ccagttgaac gtcgggttca cgctgggcag caggttgctg ccggtggagc
      781 gatcgcggtc ggcgatgccg gcggcgatgc tctccacggt gccgtccagg cgcgcgccgc
      841 ccatgggcgt gacgcgcacg cggtcgccca cgtggatgcg cggcagcttg gtctcctcga
      901 aatacccctc gacgtagaac gagcgcgcat ccaccagcgc cagtgcggcg cggcccgcct
      961 gcgcgtagct gccgctgcgc atgtccaggt tggtgacgag gccgtcggcc ggcgcccgca
     1021 cctgcgcgcg ctcgaggttc aaccgcgccg catcgagcgc cacgccggcc tgcgccacgc
     1081 cggcctgcgc ggcctcgacg cgcgtggcgg tctgctcgcg cagttcctgc gagatcaggt
     1141 tgcccaggtc gacgttgcgc gcgttctcgc gccgcgcctg ggccagcgtc accttcgcgg
     1201 cggtgagcga cgcctgggcc tggcgcagcg cgagctcgta gcgcgcgcgg tcgacctcga
     1261 acagcagcgc gccggcggcc acgcgctggt tgtcctgcac cggcaccgcg gtgaccaggc
     1321 ccgagacgtc cggcgcgatc tggacgacgt tggcgcgcac gcggccgtcg cgcgtccacg
     1381 gctcgagctc gtagtgatcc cacaggcgcg acgcggccca cgcggcggcg agcaccacgg
     1441 cgagcgtcac gagcaggcgc gcgaggcgca tcggccagcg gccgggggcg agaagatcgg
     1501 aagtcatggc gggactcaat gcgtttgcaa cagggaggcg gcgctgctga cgacgtacag
     1561 cagcaggagg tacagcgcag cgtcgaacag cgccgggtgc cacacccagc ggtagaagcc
     1621 gatggcgccg agcaggcggt ggacgagcca ttgcacggcc agcgtgaggc cgcacagcag
     1681 catcagccag ggcacgtaca ggccatggaa ggcggcttcg ccgatcatgc gaggactccg
     1741 gtggacggtg tgacgagtgc cgcgggagcg gcagggaaca gcgcgcggcg caggccgacg
     1801 agcgcggtga cggccgcgcg ggtgtcgcgg ccgtcgccgg ccagcgcgcg cgcgagagcg
     1861 ccgtcgaagt cggccagcag cgacgccggc ggcgcgccgt cgcgtcccgc gccacgcagg
     1921 cggaacaggc gggcgacgcc gcccagcacg gcgccgatgc ccatcgaggg caggcgcgga
     1981 cgcgcgcgct gcagcgccac gatgtccagg cccgagcgca ggtcgcgcag cgcgccctcg
     2041 acggtgtcgt tgcgcaccgc cgggtcggcc tgcgccacgc gcggcgcgag cagcgcgatg
     2101 cggtcgacca tgcgcaccgc gtacgcgtcg tccggcggcg cgccgcgcgg cgcgtcggcc
     2161 atcgcgccga gctcgctcca cgtcgcgcgg cggatgcgct gcgccgtcca gtcggcgccg
     2221 acggtgcgca ccaggcgcgt caccagcgcg gcggcgtaga tgcccagcac ctgggcgagc
     2281 agcgaattga cgaaggcggg cagttcgacg gtggccgagg tgtcgtgcat cgcgagcgtg
     2341 cccgcgacgc cgaagatcat cgccatcgcg gaaggcgccg tggcaggacg ggcgcagaac
     2401 agtcccagca g
//
LOCUS       DATCRO010000959         9475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_528503, whole genome shotgun sequence.
ACCESSION   DATCRO010000959 DATCRO010000000
VERSION     DATCRO010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528503"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VIN75_26230"
     CDS             <1..662
                     /locus_tag="VIN75_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8977740.1"
                     /translation="ADTRLRALILRFVREYADAQNEHRVLTEDVRFLEPEERVDSPLN
                     QGILDRAKLAEFLQGLIKADHIDHAFVCGPFMMNDEAEAALLAAGVAADRIHIERFGV
                     PIPADSGFGAQPAPVPAPAPGDAPDAQLTVVRDGVTHQIEFHEADNNLLDACAHAGLD
                     VPYSCKSGVCGTCRAHLVSGEVRMTRNYALEKRDLDAGFILTCQAHPTTAEVKISFDH
                     R"
     gene            683..1345
                     /locus_tag="VIN75_26235"
     CDS             683..1345
                     /locus_tag="VIN75_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8977741.1"
                     /translation="MARTRSATYDDQRDTILAQAAKLFAEQGYTAASMNDIAAACGVS
                     KAGLYHYYRDKRELLFQITAGHVARLEQFVTEVLEETRGADADTRLRALILRFVREYA
                     DAQNEHRVLTEDVRFLEPEERARVQEPQRRVVAAFADAIAAVRPNLARNELDRPLAML
                     LFGMINWMFTWLRPDGSLTHEQMAPLVADLFFGGLAAVRKPRGAARRDAAVPSLSPTT
                     AD"
     gene            1360..2517
                     /locus_tag="VIN75_26240"
                     /pseudo
     CDS             1360..2517
                     /locus_tag="VIN75_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539784.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2671..4731
                     /gene="paaZ"
                     /locus_tag="VIN75_26245"
     CDS             2671..4731
                     /gene="paaZ"
                     /locus_tag="VIN75_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY8977742.1"
                     /translation="MKQLQSYIGGRFVGTRAGAALASAVDGRVVAHAHADEIDFGEAV
                     AHARRVGVPALLALDFQQRAARLKALAKFLMERKEDLYAISAHTGATRADSWIDIEGG
                     AGTLFAYASMGSNELPSGNLIHEGPAMPLGKKGQFAGTHVLVPRRGVAVHINAFNFPV
                     WGALEKFAPTFLAGMPSIVKPATSTSYLTEAMVRLAVESGLLPEGALQLVVGSTGDLL
                     DRVDGRDIVTFTGSADTAAKLRVRPNLIASSVPFNAEADSLNCAVLADDVTPDDVEFD
                     LFVKEVAREMTVKAGQKCTAIRRAIVPRRHLQAVTERLKARLAKVVVGDPSLEGVTMG
                     ALASRDQQRDVAERVAQLARGNAVLFGGEAGFAPRGSGTDQGAFFSPTLLLCERPDMQ
                     SAAHDVEAFGPVSTLMPYDEFDEALALAERGRGSLVGTLVTRDPHRAAHAVSRLAALH
                     GRLLVLDREAAGESTGHGSPLPALKHGGPGRAGGGEELGGIRAVKHYLQRAAVQGSPT
                     MLAAVTGEHVKGAAVHESGVHPFRHAFEDLTVGDSLLTHRRTVTDADIVAFGGISGDF
                     FYMHFDEIAAKASPFGKRIAHGYFVLSAAAGLFVSPAEGPVLANYGLDTLRFVKPVGI
                     GDTIRARLTCKRKIDRNKVGADGRGQGVVAWDVEVTNQDGEVVASYDILTLVAKREVP
                     AAAL"
     gene            4931..5161
                     /locus_tag="VIN75_26250"
     CDS             4931..5161
                     /locus_tag="VIN75_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977743.1"
                     /translation="MSKQEVKTTLGADGLRYRSKAAGSPFAATGAFGAATMSCFLCGK
                     HRPRAQLKTRKLLGRAQSVCAPNCKEADAPAA"
     gene            5412..6128
                     /locus_tag="VIN75_26255"
     CDS             5412..6128
                     /locus_tag="VIN75_26255"
                     /inference="COORDINATES: protein motif:HMM:NF025501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4303 domain-containing protein"
                     /protein_id="HEY8977744.1"
                     /translation="MSFVASPMPTLDSPLLLATLRDELRAAWHAFRALPGHERLYGFG
                     VYTTDSASYLTATAFSEAGLDAVAAKYRAGEFGEGRDPALLRESLRWSPADSPLHAEG
                     LELLSRSDAMVQELDFEGRWDEDDDGDDDDDDDGDDDDLDADDERDPDVQEVFRVAAQ
                     ALRELDAEGLFGSGDDRERLVLCIWEGDQSNEERYRHARSLNPPSVARRFGEEMNAGL
                     RAYYRAYMPEEEAPEDDVFE"
     gene            6251..6760
                     /locus_tag="VIN75_26260"
     CDS             6251..6760
                     /locus_tag="VIN75_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977745.1"
                     /translation="MLPNPFSPPRAVVADMSFGADMRKPVWVWLVQAMALLAAGACAL
                     TLDVLVPIVRFGRADARFLAASAMATGVIVCAGFALAGSQQRRAYGRWCGLALPASIV
                     LKQGSTCMEWLDAMAGDAGESGLATADVAVIVATVAGMGLAVLPGWAYGFSRRCLSWF
                     GVTAQAASG"
     gene            6757..7083
                     /locus_tag="VIN75_26265"
     CDS             6757..7083
                     /locus_tag="VIN75_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8977746.1"
                     /translation="MTSDGRPVPDPDCKHPAGRFAPVIDPNRCEGKSACAAECPYDVF
                     VVRRRERAELPGLDLLGAVKWWLHGGLQAEAVKADRCHGCGKCVTACPESAITLARVT
                     GQAPPA"
     gene            7256..8416
                     /locus_tag="VIN75_26270"
     CDS             7256..8416
                     /locus_tag="VIN75_26270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977747.1"
                     /translation="MTSSSSIAPSVPDRAGIRRSQLAVASLFGALGFSYGTWTSRLPA
                     LKANLGMSTSQVSVMMLCAAFGALFSFPVTAGALHKLGSRGASLVSGALLAVGLAALG
                     IAPSWIAACVVMCIYGVVASTLDVAMNAQGVEVERATGEAVMSRLHAVFSLGTMCGAF
                     FAAAITNVATSLPLHFVIAAGVVLAAVLLPRAGLVADARPIEGGTRSFALPRGAALLI
                     GAMAFLGMIAEGSMVDWSTLYLRERAGATEQVAPYGIASLQGAMLAARWFGDRARVRW
                     GARKLLFAGSLLASCSLAAALLIGGVVPALVAFGLFGIGVATVSPCVYAAGAREGGVA
                     LAAVMTLGSLGFLVAPPLIGAVSQATNLSWGLSVVAAAAFALALLSLRVRWD"
     gene            complement(8455..9054)
                     /locus_tag="VIN75_26275"
     CDS             complement(8455..9054)
                     /locus_tag="VIN75_26275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8977748.1"
                     /translation="MIAPGTTAFYDAWAADGAEAERSAVSRLFAASFAPGARVLDVGC
                     GKGRDVVALLDMGFDAWGIEPNDAMRARALARDPRLAGRIAPATLPGLGQPFGGGFDG
                     VVCCAVLMHLSPDDLPATLAALVSVLRPQGRLLLALPEMRADLLVDGRDPDGRQFANH
                     APERVEALLARLGLALVRCEEIATSSTDTRWRVLLFGRG"
     gene            complement(9051..9299)
                     /locus_tag="VIN75_26280"
     CDS             complement(9051..9299)
                     /locus_tag="VIN75_26280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977749.1"
                     /translation="MSTDVAARLAGLSRKEWTLIALGLATWVAIITGGVWFVHQMTRS
                     TAACGTQLEARSLKPATTPGAGPRNALQLLSGTGKCPR"
BASE COUNT         1337 a         3378 c         3375 g         1385 t
ORIGIN      
        1 acgccgacac gcggctgcgc gcgctgatcc tgcgcttcgt gcgcgagtac gccgacgcgc
       61 agaacgaaca ccgcgtgctg accgaggacg tgcgcttcct cgagcccgag gagcgcgtgg
      121 attcgccgct gaaccagggc atcctcgacc gcgccaagct ggccgaattc ctgcaggggc
      181 tcatcaaggc ggaccacatc gaccatgcgt tcgtctgcgg cccgttcatg atgaacgacg
      241 aggccgaggc cgcgctgctg gccgcggggg tcgcggccga ccgcatccac atcgagcgct
      301 tcggcgtgcc gattccggcc gactcgggct tcggcgcgca gcccgcgccg gtgccggcac
      361 ccgcgcccgg cgacgcgccc gacgcgcagc tcaccgtcgt gcgcgacggc gtcacgcacc
      421 agatcgagtt ccacgaggcc gacaacaacc tgctggacgc ctgcgcccac gccggcctgg
      481 acgtgccgta ctcgtgcaag tcgggcgtgt gcggcacctg ccgcgcccac ctcgtttcgg
      541 gcgaggtgcg catgacgcgc aactatgcgc tcgagaagcg cgacctggac gccggcttca
      601 tcctcacctg ccaggcgcac ccgacgacgg ccgaagtcaa gatctcgttc gaccaccgct
      661 gaacgtcgct gacaatcgac cgatggcccg cacccgctcc gccacctacg acgaccagcg
      721 cgacaccatc ctcgcgcagg cggccaagct gttcgccgag cagggctaca ccgcggcgtc
      781 gatgaacgac atcgcggcgg cctgcggcgt gtccaaggcc ggcctgtacc actattaccg
      841 cgacaagcgc gagctcctgt tccagatcac ggccggccac gtcgcgcggc tggagcagtt
      901 cgtcaccgag gtgctggagg agacgcgcgg cgccgacgcc gacacgcggc tgcgcgcgct
      961 gatcctgcgc ttcgtgcgcg agtacgccga cgcgcagaac gaacaccgcg tgctgaccga
     1021 ggacgtgcgc ttcctcgagc ccgaggagcg cgcccgcgtg caggagccgc agcgccgcgt
     1081 ggtggcggcc ttcgccgacg cgatcgccgc cgtgcgcccg aatctcgcgc gcaacgagct
     1141 cgaccggccg ctggcgatgc tgctgttcgg catgatcaac tggatgttca cgtggctgcg
     1201 ccccgacggc agcctcaccc acgagcagat ggcgccgctc gtggccgacc tgttcttcgg
     1261 cgggctggcc gcggtgcgca agccccgcgg cgcggcgcgc cgggatgccg ccgttccttc
     1321 cctctcaccg acgacagctg attgatggag atgaccgaga tgaacaagaa ccgcttcccg
     1381 ttccaggccc tgctggccgc cgtgctggcg ctggccgccg gggccgcgct ggccgacatc
     1441 aacgtcggcg tcacggtgtc ggccaccggc ccggccgcgt ccctgggcat ccccgagaag
     1501 aacaccatcg cgctgatgcc ccaggagatc gccggccaga aggtgcacta catcgtgctg
     1561 gacgacggct cggacaccac caaggcggtg agcaacacgc gcaagctgat caccgaggac
     1621 aaggtcgacg tcatcctcgg atcgaccgtc acgcccaact cgctggcgat gatcgacgtc
     1681 gtggccgagg cgcaggtgcc gatgatctcg atggcggcct ccgccaagat cgtcgacccg
     1741 gtggacgcca agcgccgctg ggtgttcaag acgccgcaga acgacatcca gatgtcgctg
     1801 gccatcgcgt cgcacatgaa ggaccacggc gtcaagacgg tgggctacat cggttttgcc
     1861 gacgcgtacg gcgagggctg gtacaacgag ttctccaagg ccgcggcgct caagggcatc
     1921 acggtcgtcg ccaacgagcg cttcgcgcgc aacgactcgt cggtcaccgc gcaggtgctc
     1981 aagctgcagg ccgccaggcc cgacgcgatc ctgatcggcg gttccggcac gccggccgcg
     2041 ctgccgcaga tcacgctcaa ggagcgcggc tacacggggc tgatctacca gacgcacggc
     2101 gtggccaacg ccgacttcct gcgcgtgggc ggcaagaacg tcgagggcac ctacctgccc
     2161 gcgggcccgg tgctggtcgc caggcaactg cccgcctcca acccggtgcg caactccgcc
     2221 atcgagtacg tcacgaagta cgaggcgatg tacggcaagg acagcgtgtc gaccttcggc
     2281 ggccacgcct gggacgccgg caagctgatg gccgccgccg tgcccgtcgc gctcaagaag
     2341 gcgcagcccg gcacgcccga attccgcgcc gcgctgcgcg acgcgctgga aagcacctag
     2401 gacgtggccg gcgcgcacgg catcttcaac atgagcccga ccgaccacct gggcctggac
     2461 cagcgcgctc gcgtgatggt caagatcgag aacggcacct ggaaggtgca gccgtgagcg
     2521 gctcgcgggc cggccaggcg ccggcccgtc gttcctccag aagcatttcc aactcaggac
     2581 tttcatcatg agccagtcga gtccgcacgg ccgtccgaag gacgagagcc ccctctcgga
     2641 ggggaggcgc acagcgccag gggagcccac atgaagcagc tgcagagcta catcggcggc
     2701 cgcttcgtcg gcacgcgcgc gggcgcggcg ctcgcgagcg ccgtcgacgg gcgcgtcgtc
     2761 gcgcatgcgc acgccgacga gatcgacttc ggcgaggccg tggcccacgc gcgccgcgtg
     2821 ggcgtgcccg cgctgctggc gctggacttc cagcaacgcg ccgcgcgact gaaggcgctg
     2881 gccaagttcc tgatggagcg caaggaagac ctgtatgcga tctccgcgca caccggcgcc
     2941 acgcgcgccg acagctggat cgacatcgag ggcggcgccg gcacgctgtt cgcgtacgcg
     3001 agcatgggca gcaacgagct gccgtcgggc aacctgatcc acgaaggccc ggcgatgccg
     3061 ctgggcaaga agggccagtt cgccggcacg cacgtgctgg taccgcggcg cggcgtcgcg
     3121 gtgcacatca acgcgttcaa tttcccggtc tggggcgcgc tggagaagtt cgcgccgacc
     3181 ttcctcgcgg gcatgcccag catcgtcaag cccgccacct ccaccagcta cctcaccgag
     3241 gcgatggtgc gcctggccgt cgaatcgggc ctgctgcccg aaggcgcgct gcagctggtg
     3301 gtggggtcga cgggcgacct gctcgatcgc gtcgacggcc gcgacatcgt caccttcacg
     3361 ggctcggccg acaccgccgc gaagctgcgc gtgcgtccga acctgatcgc gagcagcgtg
     3421 ccgttcaacg ccgaggccga ctcgctgaac tgcgcggtgc tggccgacga cgtcaccccc
     3481 gacgacgtcg agttcgacct cttcgtcaag gaggtggcgc gcgagatgac cgtcaaggcc
     3541 ggccagaagt gcacggcgat ccggcgcgcg atcgtgccgc gccgccacct gcaggccgtc
     3601 accgagcggc tgaaggcgcg gctcgccaag gtcgtcgtcg gcgatccttc gctggagggc
     3661 gtgacgatgg gcgcgctcgc ctcgcgcgac cagcagcgcg acgtcgccga acgcgtggcg
     3721 cagctggcgc gcggcaatgc cgtcctgttc ggcggcgagg cgggcttcgc gcccaggggc
     3781 agcggcaccg atcagggcgc gttcttctcg ccgacgctgc tgctgtgcga gcgcccggac
     3841 atgcaatcgg ccgcgcacga cgtcgaggcc ttcggccccg tcagcacgct gatgccctac
     3901 gacgagttcg acgaggcgct ggcgctggcc gagcgcggcc gcggcagcct ggtgggcacg
     3961 ctggtcacgc gcgacccgca ccgcgccgcc cacgccgtga gccgcctcgc ggcgctgcac
     4021 ggccggctcc tggtgctcga tcgcgaggcc gcgggcgagt ccaccggcca cggctcgccg
     4081 ctgcccgcgc tcaagcacgg cggcccgggc cgcgccggcg gcggcgagga actgggtggc
     4141 atccgtgccg tcaagcacta cctgcagcgt gccgcggtgc agggctcgcc gacgatgctg
     4201 gccgcggtga ccggcgagca cgtcaagggc gcggcggtgc acgagtcggg cgtccatccg
     4261 ttccgccacg cgttcgagga cctgaccgtg ggcgactcgc tgctgacgca tcgccgcacc
     4321 gtgaccgatg ccgacatcgt cgccttcggc ggtatctcgg gcgatttctt ctacatgcac
     4381 ttcgacgaaa ttgccgcaaa ggcgtcgccg ttcggcaagc gcatcgcgca cggctacttc
     4441 gtgctgtccg cggcggcggg gctgttcgtg tcgccggccg aaggcccggt gctcgcgaac
     4501 tacggcctgg acacgctgcg cttcgtcaag ccggtgggca tcggcgacac gatccgcgcg
     4561 cgcctgacct gcaagcgcaa gatcgaccgc aacaaggtcg gcgcggacgg gcgcgggcag
     4621 ggcgtggtgg cgtgggacgt cgaggtcacc aaccaggacg gcgaggtcgt ggcgagctac
     4681 gacatcctca ccctggtcgc caagcgcgag gttccggccg ccgccttgtg aaggcagggg
     4741 cctcgccggc accgccaagc cccatggtcg agttctggca cgggccttgc acgaccggaa
     4801 tgtccctgcg tcagctggct gagggtatat acagtcaatc tgttagaatg tcggatggcg
     4861 cgaggaagga cgtgcggctc tccggccaag cgtacgcgcc ctgttcctat cccggaagga
     4921 tcatcactcg atgtcgaagc aagaagtcaa gaccaccctc ggcgccgatg gcctgcgcta
     4981 tcgctccaag gccgcgggtt ccccgttcgc agccaccggc gctttcggcg ccgccacgat
     5041 gtcgtgcttc ctgtgcggca agcatcgtcc tcgtgcccag ctcaagaccc gcaagctgct
     5101 gggccgcgcc cagtcggtct gcgcgcccaa ctgcaaggaa gccgacgctc ccgcagcttg
     5161 atggctgacg gcggagtgct ggtcgggctc cgccacacgc ttcacgaacg cacgccaccc
     5221 ggcgtgcgtt ttgtttttgt ggcgccggtt cctcgggctg ggcgtgcccg cgcaggtctc
     5281 cgaccgggat ggccgccagc gacggcgtct ggagcaggtc gtgggcggcg cgctccggcg
     5341 tggcaaacgg gccggaaacc cgtcaaatgg cgccgggcat gtttcgccag ggggcctatg
     5401 ctgcaggttc gatgtccttc gttgcctcgc ccatgcccac gctcgattcc cctttgctcc
     5461 tggccacgct gcgcgacgaa ctgcgcgccg cctggcatgc gttccgtgcc ctgccgggcc
     5521 acgaacggct gtacggcttc ggcgtgtaca ccaccgattc ggccagctac ctgacggcca
     5581 ccgccttcag cgaggccggc ctggacgccg tcgcggccaa gtaccgcgcc ggcgagttcg
     5641 gagagggccg cgatccggcg ctgctgcgcg aatccctgcg ctggagcccg gccgactcgc
     5701 cgctgcacgc cgagggcctg gaactgctct cccgttcgga cgccatggtg caggagctcg
     5761 acttcgaagg ccgctgggac gaggacgatg acggcgacga cgacgacgac gacgacgggg
     5821 acgacgatga cctggatgcc gacgacgagc gcgaccccga cgtgcaggag gtcttccgcg
     5881 tcgcggcgca ggccctgcgc gaactcgatg ccgaggggct cttcggcagc ggcgacgacc
     5941 gcgaacgcct ggtgctgtgc atctgggaag gcgaccagtc caacgaggaa cgctaccgcc
     6001 atgccaggtc gctgaatccg ccgtcggtgg cccggcgctt cggcgaggaa atgaatgcgg
     6061 ggctgcgggc gtactaccgg gcatacatgc cggaagagga ggcgccggag gacgacgtgt
     6121 tcgagtgaag gggccccagt ggcgtgcgcg ccgggctgtc cggagccgcg tgggcgggag
     6181 gctatgcttg tcgaacgatt ctcccctcgc acgtctcttc cgtggcgcgc ctgctgctcg
     6241 tccgttcccg atgctgccca atcccttttc gcctcctcga gccgtcgtcg cggacatgtc
     6301 ctttggtgcc gacatgcgca agccggtgtg ggtgtggctc gtgcaagcga tggcgctcct
     6361 ggcggcgggc gcctgtgcgc tgacgttgga cgtcctggtg ccgatcgtcc gattcggccg
     6421 ggccgatgcc cgcttcctgg cggcgtccgc gatggcgacg ggcgtcatcg tgtgcgcggg
     6481 gttcgcgctg gccgggagcc agcaacgccg ggcctacggc cgctggtgcg gcctcgcgct
     6541 gccggcgagc atcgtcctga agcaaggctc gacgtgcatg gagtggctcg acgcgatggc
     6601 gggagacgcc ggcgaatccg ggttggcgac agcggacgtc gcggtgatcg tcgcgacggt
     6661 tgccggcatg ggcctggcgg tgctcccggg ctgggcttac ggcttctcgc ggcgctgcct
     6721 ctcgtggttc ggcgtgacgg cgcaggcggc gtccggatga cgtcggacgg ccgtcccgtt
     6781 ccggatccgg actgcaagca cccggcaggc cgcttcgcgc ccgtcatcga ccccaaccgc
     6841 tgcgaaggca agtccgcctg cgccgccgag tgcccgtacg acgtcttcgt cgtgcgccgg
     6901 cgcgagcgcg ccgagttgcc cgggctggac ctgctgggcg cggtcaagtg gtggctgcac
     6961 ggcggcctgc aggccgaggc cgtgaaggcc gaccgctgcc atggctgcgg caagtgcgtc
     7021 acggcgtgcc cggagagtgc gatcacgctc gcgcgcgtca ccgggcaggc gccgccggcg
     7081 tgaatcctgc ggccaggcgg catgtgccgc tggcgcaccg aacgatatcc ggtgcgcggc
     7141 gggtcgggcg gggcgccatc tacccgcaca atcggcacgg gctcgaggcc gtcgtgagtg
     7201 gacaccagac ccgcgatccg ccgtggcatc gaacgcccgg ccatcctcat cgaacatgac
     7261 gtcgtcttcc tccatcgccc cgtccgtgcc cgaccgggcc ggcatccgcc gcagccaact
     7321 ggccgtcgcg tcgctgttcg gcgcgctggg cttcagctac ggcacctgga cctcgcggct
     7381 gccggcgctc aaggccaacc tcggcatgag cacctcgcag gtcagcgtga tgatgctgtg
     7441 cgcggcgttc ggcgcgctgt tctcgttccc ggtgacggcc ggcgcgctgc acaagctggg
     7501 ctcgcgtggc gccagcctgg tgtcgggggc gctgctggcg gtcggcctcg cggccctggg
     7561 catcgcgccc agctggatcg cggcctgcgt cgtcatgtgc atctacggcg tcgtggccag
     7621 cacgctggac gtggcgatga acgcgcaggg cgtcgaagtg gagcgggcga ccggcgaggc
     7681 ggtgatgtcg cggctgcacg cggtcttcag cctgggcacg atgtgcggcg cgttcttcgc
     7741 ggcggccatc accaacgtcg ccacctcgtt gccgcttcac ttcgtcatcg ccgccggcgt
     7801 ggtgctggcc gcggtcctgc tgccgcgcgc gggtctcgtc gcggacgcca ggccgatcga
     7861 gggcggcacg cgttcgttcg cgctgccgcg cggcgcggcg ctgctgatcg gggcgatggc
     7921 cttcctgggg atgatcgccg agggctcgat ggtggactgg tcgacgctgt acctgcgcga
     7981 gcgcgccggt gccacggaac aggtcgcgcc ctacggcatc gccagcctgc agggcgcgat
     8041 gctggccgcg cgctggttcg gcgaccgggc ccgcgtgcgc tggggcgcgc gcaagctgct
     8101 gttcgccggg tcgctcctgg cgtcgtgcag cctggcggcg gcgctgctga tcggcggggt
     8161 cgttccggcg cttgtcgcct tcggcctgtt cggcatcggc gtggccacgg tgtcgccgtg
     8221 cgtgtacgcg gcgggcgcgc gcgagggcgg tgtcgcgctg gcggcggtga tgacgctggg
     8281 ctcgctcggc tttctcgtcg cgccgccgct gatcggcgcg gtctcgcagg ccaccaacct
     8341 gtcctggggg ctgtccgtgg tcgcggccgc ggcgttcgcg ctggcgttgc tgtcgctgcg
     8401 ggtgcggtgg gattgacggc ccgagctgcc ctctcgccgg cgttccggcc cgcctcagcc
     8461 tcgcccgaac aagagcaccc gccaccgcgt gtcggtcgac gacgtggcga tctcctcgca
     8521 gcgcaccaac gccagtccca accgggcgag caacgcctcg acgcgctcgg gcgcgtggtt
     8581 ggcgaactgc cggccatcgg gatcgcggcc gtcgaccagc aggtctgcgc gcatctcggg
     8641 caaggccagg agcaggcggc cctgcgggcg caatacggac accagcgccg ccagcgttgc
     8701 cggcaggtcg tcgggcgaca ggtgcatcag caccgcgcag cagacgacgc cgtcgaagcc
     8761 gccgccgaac ggctgcccca ggccgggcag ggtggcggga gcgatgcggc cggccaggcg
     8821 cgggtcgcgc gccagcgcgc gggcgcgcat cgcgtcgttc ggctcgatgc cccacgcatc
     8881 gaaccccatg tccagcaagg cgacgacgtc gcggcccttg ccgcagccca cgtccagcac
     8941 gcgcgcaccg ggcgcgaacg aggctgcgaa caggcgcgag accgcgctgc gctcggcctc
     9001 ggcgccgtcg gcggcccagg cgtcgtagaa ggccgtggtg ccgggcgcga tcatcgcggg
     9061 cacttgccgg ttccgctcag caactgcagc gcgttgcgcg gccccgcgcc gggcgtggtc
     9121 gccggcttca ggctgcgggc ctcgagctgc gtgccgcagg cggcggtgct gcgcgtcatc
     9181 tggtggacga accacacgcc gcccgtgatg atcgccaccc acgtggccag gccgagcgcg
     9241 atgagcgtcc attccttgcg cgagagtccc gccaagcgtg cggcgacgtc tgtcgacatg
     9301 cctgttctcc tccctggtcg tgtcgcgcgt gcagcttaac ccgccgcggc caggcgaccg
     9361 gtcgcgcaca atggcatttc cgacgcggca gctcccccgt aggcgcggcg ggggacaatc
     9421 aggccatgct ccttcgaatg ttctcggcag ggacgcccgt cgccctgcgc acgac
//
LOCUS       DATCRO010000960         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_494775, whole genome shotgun sequence.
ACCESSION   DATCRO010000960 DATCRO010000000
VERSION     DATCRO010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494775"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VIN75_26285"
     CDS             complement(<1..820)
                     /locus_tag="VIN75_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY8977750.1"
                     /translation="MSLKLPQGMTISGAIQPGFETILTPEALAFVADLHRTFEGRRKE
                     LLAARVERTARLDAGERPDFLPSTAHIRNGDWKVAPLPPALACRRVEITGPVEAKMVI
                     NAFNSGADSYMTDFEDSNAPKWENQIQGQINLGQAIRRTLKLEQNGKSYKLNDRIATL
                     QVRPRGWHLDEKHVLVDGQRVSGGIFDFGLFLFHNAREQIARGAGPFFYLPKMESHLE
                     ARLWNDIFVRAQDKLGLPQGTIKATVLIETILAAFEMDEILYELREHSAGLNAGR"
     gene            complement(932..1630)
                     /locus_tag="VIN75_26290"
     CDS             complement(932..1630)
                     /locus_tag="VIN75_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY8977751.1"
                     /translation="MASNPASPHRPTAPKAVLFDVYGTLLDVYSVALRAEQMFPGAGE
                     RLARAWRDRQIEYSRLVSMSGRYRPFWQLTRDALQVSAAALRLPLDDAGADSLMNEYR
                     HLSAFPENRGVLQALADRGIRAGILSNGDPEMLAVAIRSAGLTELIDPILSVHATQRY
                     KTDPAAYAIGPAALGLPASDILFVSSNCWDAIGATWHGFTTLWVNRADAPMERLGIEP
                     TRVGRSLRDVLEFF"
     gene            1710..2621
                     /locus_tag="VIN75_26295"
     CDS             1710..2621
                     /locus_tag="VIN75_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977752.1"
                     /translation="MDKLKQMETFVAVAQRGSLTAAAQAEGVAPAVISRRLDGLEARL
                     GVKLLARTTRRVSLTHEGSAFLEDCQRLLQDFASAEASVSAGGVKASGHLRITAPAGF
                     GRRHVAPLVPQFLAEHPDVSLSLNLSDRVVDIVNEGVDCAVRVGDLQDSSLVSVRLAD
                     NRRLCVAAPGYLKRAGTPRVPADLARHACLTLSSEASQTRGWAFTVAGALTHLRLNGR
                     LDCTDGQVLHDWCVAGLGVAWRSAWEVGADLAEGRLVSVLDEFAAPPNGIYAVFPHAR
                     HLALRVRLWIDFLKHTWGDARYWNRAS"
BASE COUNT          376 a          895 c          968 g          419 t
ORIGIN      
        1 agcggccggc gttcaggccc gcgctgtgtt cgcgcagctc gtacaggatc tcgtccatct
       61 cgaacgcggc gaggatggtc tcgatcagca cggtggcctt gatcgtgccc tgcggcaggc
      121 ccagcttgtc ctgggcgcgc acgaagatgt cgttccacag gcgcgcctcg aggtggctct
      181 ccatcttggg caggtagaag aacgggccgg cgccgcgcgc gatctgctcc ctggcgttgt
      241 ggaacaggaa caggccgaag tcgaagatgc cgcccgagac gcgctggccg tcgaccagca
      301 cgtgcttctc gtcgaggtgc cagccgcgcg gacgcacctg cagcgtggcg atgcggtcgt
      361 tgagcttgta gctcttgccg ttctgctcca gcttgagcgt gcggcggatc gcctggccca
      421 ggttgatctg gccctggatc tggttctccc acttcggggc gttgctgtcc tcgaagtcgg
      481 tcatgtagct gtcggcgccc gagttgaacg cgttgatgac catcttggcc tcgaccgggc
      541 cggtgatctc cacgcggcgg caggcgagcg cgggcggcag cggcgcgacc ttccagtcgc
      601 cgttgcggat gtgcgccgtc gacggcagga aatcggggcg ctcgcccgcg tccaggcgcg
      661 cggtgcgctc gacgcgcgcg gccagcagct ccttgcggcg accctcgaac gtgcggtgca
      721 ggtcggcgac gaaggccagc gcctccggcg tgaggatggt ctcgaagccg ggctggatcg
      781 cgccggagat ggtcatgccc tggggcagct tgaggctcat ggaaagctcc tgaaggttgg
      841 aatcgaagat gggtggctag tcaggggcga gtgcgtgagc ctgggtgggc ccgggtcgac
      901 cggcgggctg ttgacccgcc accggccgca ctcagaagaa ttcgaggacg tcgcgcaacg
      961 agcgcccgac gcgcgtgggt tcgatgccga gccgctccat cggcgcgtcg gcgcggttga
     1021 cccacagggt ggtgaagcca tgccaggtcg cgccgatcgc gtcccagcag ttggacgaga
     1081 cgaacaggat gtccgacgcc ggcaggccca gcgccgccgg gccgatcgcg taggcggcgg
     1141 ggtcggtctt gtagcgctgc gtggcatgca cgctcaggat cgggtcgatc agctccgtga
     1201 ggccggcgct gcggatggcc acggccagca tctcggggtc gccgttggac aggatgcccg
     1261 cgcggatgcc gcggtcggcc agcgcctgca gcacgccgcg gttctcgggg aacgcggaca
     1321 ggtgccggta ttcgttcatc aggctgtcgg cgccggcatc gtccagcggc agtcgcaggg
     1381 cggcggccga cacctgaagg gcgtcgcgcg tcagctgcca gaacggccgg tagcggccgc
     1441 tcatcgacac caggcgcgag tactcgatct ggcggtcgcg ccacgcgcgc gccaggcgct
     1501 cgcccgcgcc gggaaacatc tgctcggcgc gcagcgccac gctgtagaca tccagcagcg
     1561 tcccgtagac gtcgaacagc accgccttcg gggcggtcgg acgatggggc gatgcggggt
     1621 tggaggccat tcccgaaatc tatttcatcc tcgaatggcg attcccacca taatttgcga
     1681 ttgattgatg actttttcgt aaaactgccg tggacaagct caagcagatg gagaccttcg
     1741 tggcggtcgc ccagcgcggc agtctgaccg cggccgccca ggccgagggc gtggcgcccg
     1801 ccgtcatcag ccgccggctc gatggcctgg aggcgcggct gggcgtcaag ctgctggcgc
     1861 gcacgacgcg gcgcgtgtcc ctgacgcacg aaggcagcgc gttcctggag gactgccagc
     1921 ggctgctgca ggacttcgcc agcgccgagg ccagcgtctc ggccggcggc gtcaaggcga
     1981 gcggccacct gcgcatcacc gcgccggccg gcttcggccg ccgccacgtg gcgccgctgg
     2041 tgccgcagtt cctggccgaa cacccggacg tgagcctgtc gctgaacctg tcggaccgcg
     2101 tggtcgacat cgtcaacgaa ggcgtcgact gcgcggtgcg cgtgggcgac ctgcaggact
     2161 ccagcctggt cagcgtgcgc ctggccgaca accgtcgcct gtgcgtcgcc gcgcccggtt
     2221 acctcaagcg cgccggcacg ccgcgcgtgc ccgccgacct cgcccgccac gcctgcctga
     2281 cgctcagctc cgaggccagc cagacccgcg gctgggcctt caccgtggcc ggcgcactga
     2341 cgcacctgcg cctgaacggc cgcctggact gcaccgacgg ccaggtgctg catgactggt
     2401 gcgtcgccgg cctgggcgtg gcgtggcgca gtgcgtggga agtcggcgcc gacctggccg
     2461 aaggccgcct ggtcagcgtg ctggacgagt tcgcggcgcc gcccaacggg atctacgccg
     2521 tgttcccgca cgcacgccac ctggcgctgc gggtgcggct gtggatcgac ttcctcaagc
     2581 acacgtgggg cgatgcgcgc tattggaacc gggcgtcctg aagtcgttcg ctgcctgcga
     2641 ccaatggcgg gttacgcc
//
LOCUS       DATCRO010000961         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_370950, whole genome shotgun sequence.
ACCESSION   DATCRO010000961 DATCRO010000000
VERSION     DATCRO010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370950"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /gene="groL"
                     /locus_tag="VIN75_26300"
     CDS             <1..1255
                     /gene="groL"
                     /locus_tag="VIN75_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8977753.1"
                     /translation="KKASKPTTTSKEIAQVGTISANSDDSIGKIIAEAMDKVGKEGVI
                     TVEDGKSLANELDVVEGMQFDRGYLSPYFINNPEKQVALLDNPFVLLFDKKISNIRDL
                     LPTLEQVAKAGRPLLIIAEEVDGEALATLVVNTIRGILKVVAVKAPGFGDRRKAMLED
                     IAILTGGKVIAEEVGLTLEKVTLADLGQAKRVEVGKENTTIIDGNGAAGDIEARVKQI
                     RIQIEEATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALHATRAAV
                     EEGIVAGGGVALLRARQAVGELKGDNADQDAGIKLVLKAIEAPLREIVYNAGDEASVV
                     VNAVLGGKGNYGYNAANGTYGDMIEMGILDPTKVTRTALQNAASVASLMLTTECMVSE
                     APKDDAPAMPGGGMGGMGGMGGMDM"
     gene            1486..1926
                     /locus_tag="VIN75_26305"
     CDS             1486..1926
                     /locus_tag="VIN75_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977754.1"
                     /translation="MKRRIAACALGAMLLGLGGCATGYHDAGGLLGYTGGYYDHKGPG
                     ELVKVGFAGNGFISADKVRDYLTYRCAEIAHREKHDWFAIYRNLPDAIRDYRSESKQV
                     GKMSNKPWGEVYILFFDKQEDGLLNADEVIARLKPVIDGQGAKT"
     gene            1923..2366
                     /locus_tag="VIN75_26310"
     CDS             1923..2366
                     /locus_tag="VIN75_26310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977755.1"
                     /translation="MTRVASMLLAGAALAAFGGCNVLPVYQPAAGAKTAPVQLDQARG
                     GKTACVAGQPYRLEPDDKGNVNLPLGERITLQSNFSWAGGNMTYSCHPATSFVPREGQ
                     AYTMIFEIEPENHSCTFRPYRQTTGNRIGLDFDPTIGPAQACRKP"
     gene            2394..3293
                     /locus_tag="VIN75_26315"
     CDS             2394..3293
                     /locus_tag="VIN75_26315"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEY8977756.1"
                     /translation="MTIDSPRNKFAPPNAHAVGRADDASATITQEEAWAAIIGPSNTY
                     GYVERFRRLSLGGSAAWHWPAFLTTLPWLMYRKMWIGALVYMVAPVPVLIALLAMFPR
                     FAPGVILAWWLGLALLPGVMANRWYFHHCASRIRDVRARGGSKEQMLARLDAAGGTNA
                     MAVIALGVLALGGAAVLGFTVALPVYRATHANDDIRAAVQAGFDVAAAVGRHYEQTGK
                     MPSDTDVQAMVERVPHPSAALRGVDIEPSGVLVLDVQASPTLAGKIHLVPEADSGHLY
                     WTCLTDDRKVYLPRTCRLVRSYR"
     gene            complement(3357..3950)
                     /locus_tag="VIN75_26320"
     CDS             complement(3357..3950)
                     /locus_tag="VIN75_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY8977757.1"
                     /translation="MADVTYTYTSRPLQSNTPGVQVAPMTIVLDFSSDGSTLLDWSIS
                     QPDLGTIDPSNVFVSLQKQYNDSPAIYLYTDASGKVDDWFIHAIPLDPSSTPAGADYS
                     KWALSWYGVVLGAPPLPLGLYAEDTVPGGFSVNGPGTWTSSGGVLRDFGYGDRADLAT
                     LPVPEPPAGLLALLGLAALGVVRRKARPVGPVDCQAR"
     gene            complement(4109..>4609)
                     /locus_tag="VIN75_26325"
     CDS             complement(4109..>4609)
                     /locus_tag="VIN75_26325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977758.1"
                     /translation="EHDRPPEGRVVEATIWLPRAPAPRVAPPPLPAPRPTRPRASARE
                     DADAHAIRLPVPEPQPIAQAASAAEPPASAASAPPMLTLSREQMRSIIAARKPTFAEM
                     LAAPPRPSALARLGGDDAPYEERQLSGGETEVHVHGGCFRLVPTPRAQYDPFNHGNER
                     LTAACH"
BASE COUNT          768 a         1607 c         1615 g          619 t
ORIGIN      
        1 gaagaaggct tcgaagccga cgacgacctc caaggaaatc gcccaggtcg gcacgatctc
       61 ggccaactcc gacgactcga tcggcaagat catcgccgag gcgatggaca aggtcggcaa
      121 ggaaggcgtc atcaccgtcg aagacggcaa gtcgctggcc aacgaactcg acgtcgtcga
      181 gggcatgcag ttcgaccgcg gctacctgtc gccgtacttc atcaacaacc cggaaaagca
      241 ggtcgccctg ctggacaacc cgttcgtcct gctgttcgac aagaagatct cgaacatccg
      301 cgacctgctg ccgacgctgg aacaggtcgc caaggccggc cgcccgctgc tgatcatcgc
      361 tgaagaagtc gacggcgaag cgctggccac cctcgtggtc aacacgatcc gcggcatcct
      421 gaaggtcgtc gccgtcaagg ccccgggttt cggcgaccgc cgcaaggcca tgctggaaga
      481 catcgccatc ctgacgggcg gcaaggtcat cgccgaggaa gtcggcctga cgctcgagaa
      541 ggtcacgctg gccgacctgg gccaggccaa gcgcgtcgaa gtgggcaagg aaaacaccac
      601 catcatcgac ggcaacggcg ccgcgggcga catcgaagcg cgcgtcaagc agatccgcat
      661 ccagatcgag gaagccacca gcgactacga ccgcgagaag ctgcaagagc gcgtggccaa
      721 gctggccggc ggcgtggcgc tgatcaaggt cggtgccgcc accgaagtcg agatgaagga
      781 aaagaaggcc cgcgtcgaag acgccctgca cgccacgcgt gcggccgtcg aggaaggcat
      841 cgtcgccggt ggcggcgtgg ccctgctgcg tgcccgtcag gcggttggcg agctcaaggg
      901 cgacaacgcc gaccaggacg ccggcatcaa gctggtcctg aaggccatcg aagcgccgct
      961 gcgcgagatc gtctacaacg ccggcgacga agccagcgtg gtcgtcaacg ccgttctcgg
     1021 cggcaagggc aactacggct acaacgctgc caacggcacg tacggcgaca tgatcgagat
     1081 gggcatcctg gacccgacga aggtcacccg caccgcgctg cagaacgccg cgtcggtcgc
     1141 ctcgctgatg ctcaccaccg agtgcatggt ctcggaagcg ccgaaggacg acgctcccgc
     1201 gatgcccggc ggcggcatgg gtggcatggg cggcatgggc ggcatggaca tgtgatgtcc
     1261 gcttccgggc gctcgggcgt ccggagccgt cgcaccgcgc gacaccacga caagggactg
     1321 cctcggcagt cccttttcat ttgcgcgcga ctcgggattc gcacgcatgc ccccggcggc
     1381 gcgcgatggc agaatcgctc cgaccgaacg ggacaccgag cgcgccgcat cgagcacgcc
     1441 cccgcccggt ccaagggcga cgccacgtcg cgagggagga cgaccatgaa gcgaaggatc
     1501 gctgcgtgcg cgctgggcgc catgctgctc gggctgggcg gctgcgccac cggctaccac
     1561 gacgccggcg gcctgctggg ctacaccggc ggctactacg accacaaggg gccgggcgag
     1621 ctggtcaagg tcggcttcgc cggcaacggg ttcatctccg cggacaaggt gcgcgactac
     1681 ctgacctacc gctgcgccga gatcgcgcat cgcgagaagc acgactggtt cgcgatctac
     1741 cgcaacctgc ccgacgcgat ccgcgactac cgcagcgaat ccaagcaggt cggcaagatg
     1801 agcaacaagc cctggggcga ggtctacatc ctgttcttcg acaagcagga ggacggcctg
     1861 ctcaacgccg acgaggtgat cgcgcggctc aagccggtga tcgacggcca gggagccaag
     1921 acatgacgcg cgtcgcctcg atgctcctgg ccggcgccgc gctggccgcc ttcggcggct
     1981 gcaacgtgct gcccgtgtac cagccggcgg ccggcgcgaa gacggcgccg gtgcagctcg
     2041 accaggcgcg cggcggcaag acggcctgcg tcgccggcca gccctatcgc ctggagccgg
     2101 acgacaaggg caacgtgaac ctgccgctcg gcgagcgcat cacgttgcag tcgaacttct
     2161 cgtgggccgg cgggaacatg acctactcgt gccacccggc caccagcttc gtgccgcgcg
     2221 aggggcaggc ctacacgatg atcttcgaga tcgagcccga gaaccactcg tgcacgttcc
     2281 ggccctaccg ccagacgacc gggaaccgca tcggcctcga cttcgatccg accatcggcc
     2341 cggcacaggc ctgccgcaag ccctgagccg ccgccggcct tcgggaggac gtgatgacca
     2401 tcgacagccc gcgcaacaag ttcgcgccgc cgaacgcgca tgccgtcggc cgcgcggacg
     2461 acgcctccgc gacgatcacg caggaggagg cgtgggccgc gatcatcggc ccctcgaaca
     2521 cgtacggcta cgtcgagcgc ttccggcgcc tgtcgctggg cggcagcgca gcgtggcact
     2581 ggcccgcctt cctcacgacg ctgccgtggc tgatgtaccg caagatgtgg atcggcgcgc
     2641 tggtctacat ggtggcgccg gtgcccgtgc tgatcgcgct gctggcgatg ttcccgcgct
     2701 tcgcgcccgg cgtgatcctc gcgtggtggc tgggcctggc gctgctgccc ggcgtgatgg
     2761 ccaaccgctg gtacttccat cactgcgcga gccgcatccg cgacgtgcgg gcgcgcggcg
     2821 gatcgaagga gcagatgctg gcgcggctgg acgcggccgg cggcacgaac gcgatggcgg
     2881 tgatcgccct cggcgtcctg gcgctgggcg gcgcggcggt gctcggcttc acggttgccc
     2941 tgccggtgta ccgggcgacc catgccaacg acgacatccg cgcggcggtc caggccggct
     3001 tcgatgtcgc cgccgctgtc ggcaggcact acgagcagac cggcaagatg ccgtccgaca
     3061 ccgacgtgca ggcgatggtc gagcgcgtcc cgcacccgtc ggccgccctg cgcggcgtcg
     3121 acattgagcc gtcgggcgtg ctggtgctcg acgtgcaagc ctcgccgacg ctcgccggca
     3181 agatccacct cgtgccggaa gccgacagcg ggcatctcta ctggacctgc ttgacggacg
     3241 accgcaaggt ctatctgccg cgcacctgcc gcctggtgcg ctcgtatcgc tgaggcacag
     3301 cgaatcacgc gccgccgccc ggtcgggatt tcggccgacg cgcagccggc cctgtcctat
     3361 cgcgcctggc agtcgaccgg ccccaccggc cgcgccttcc tgcgcacgac acccagcgcg
     3421 gccagcccga gcagcgccag caacccggcc ggcggctcgg gcaccggaag cgtggcgaga
     3481 tcggcgcgat cgccgtagcc gaagtcgcgc aggacgccgc ccgacgaggt ccaggtgccg
     3541 ggaccgttca cgctgaagcc gccgggcacg gtgtcctcgg cgtacaggcc cagcggcagc
     3601 ggcggcgcgc ccagcacgac gccgtaccac gacagcgccc atttcgagta gtcggcaccg
     3661 gccggtgtcg acgacgggtc cagcggaatg gcatggatga accagtcgtc gaccttgccc
     3721 gacgcgtcgg tgtagaggta gatggccggc gagtcgttgt attgcttctg caggctgacg
     3781 aacacgttgg acgggtcgat ggtgcccaag tcgggctgcg agatggacca gtccagcagg
     3841 gtcgacccgt ccgacgagaa gtcgagcacg atcgtcatcg gcgcgacctg cacgccgggc
     3901 gtattggact gcagcgggcg gctcgtgtac gtgtacgtca catcggccat ggccgatccc
     3961 atgaggccgg caaggccgat ggcacagaga cagcgtttca ccttggtctc cttccacgac
     4021 gagatggcgg tgcgcggacc cgcggcaccg cctgacgggg cggcaagcga cgtgcgcgtt
     4081 cagcgatcgg caagcgcgat gacaggtctc aatgacacgc cgccgtcagc cgctcgttcc
     4141 catggttgaa cgggtcgtac tgcgcacgcg gcgtcgggac aaggcggaag cagccgccgt
     4201 gcacgtgcac ctccgtctcg ccgccgctca gctgccgttc ctcgtagggc gcgtcgtcgc
     4261 cgcccaggcg tgcgagcgcg gacggccggg gcggcgcggc cagcatttcc gcgaacgtcg
     4321 gcttcctggc cgcgatgatc gagcgcatct gctcgcgcga cagcgtcagc atcggcggcg
     4381 ccgacgcggc actggcgggc ggctcggcgg cgctcgcggc ctgggcgatc ggctgcggct
     4441 ccggtacggg gagccgtatc gcgtgcgcgt ccgcgtcctc gcgcgcggag gcgcgcgggc
     4501 gggtcgggcg cggcgcgggc aggggcgggg gcgcgacgcg cggggcgggc gcgcgcggca
     4561 gccagatcgt cgcctccacc acgcgacctt ccggcgggcg gtcgtgctc
//
LOCUS       DATCRO010000962         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_224527, whole genome shotgun sequence.
ACCESSION   DATCRO010000962 DATCRO010000000
VERSION     DATCRO010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224527"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VIN75_26330"
     CDS             complement(<1..348)
                     /locus_tag="VIN75_26330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977759.1"
                     /translation="MTLRHSLFAATILALACAGAQAQGHDHDHDRGGPPPAHGAPGKP
                     GPAPGRAGPGWSGAHARGYVDFDARYGHNHYYPSRGFVYGTLPVGAVSVGYGGGNYFF
                     HGGVWFRPYGPRYI"
     gene            complement(467..1702)
                     /locus_tag="VIN75_26335"
     CDS             complement(467..1702)
                     /locus_tag="VIN75_26335"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEY8977760.1"
                     /translation="MKIRWRVVVPVAAVGTAGLWWMASPASTPPAQVLDPVAQAAAGA
                     PAPRDVAPAASAPRRAPFSAAGLDDRAARLALWQQRLDRAKQTLAMYQAATRYPFDSR
                     PASEHADQWQTHQVVTTDAPLRMPGTGVVPNLHVHTTQQKVFATGADTVLLTVTATDD
                     NGAVLPLRVLSSMAHSPQDTGGGKAAPLAPVVTQPFVDDGSNGDAHAGDGTFTARLDP
                     GAEGFGNFAGLIRTELVVQSGDQQGYVAFDVVYAPQAPATWTGSVHDEMRDGSLAFLL
                     GIDVNQPGRYVITGRVDDANGRPVALVTFNDELRAGGGQVPLQVYGRLVRDLKPAMPL
                     TLHDVEGFLLRPDAYPDRALMPSLDGPVATSRKVAAAQFSDAPFSSDETRRYLAEYGK
                     DVTQAQQQLAQLQGPVARP"
     gene            complement(1718..2608)
                     /locus_tag="VIN75_26340"
     CDS             complement(1718..2608)
                     /locus_tag="VIN75_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977761.1"
                     /translation="MHVRLRNVSICAALVAAAGLAAVSQATNYSLWVNGRTGGGQAGN
                     YADFTYWGPGTTAAGVNKKSVNWDGYNHISDQNYLVRNALDCFCTGSNWCYVAAHSAG
                     DLMIGYTLAMYGGSSRTITNATPNSSGVCGASGAGTQTGWNIKWVDVAAGAGGGSELA
                     NVGDWATSEPLVSDLKTGTARAMYDHNNTRSHWFYMWAGAKGTLYSAVLPGQDDEAVA
                     YHSAGAVSGSSGGAYCNPSDWFCNDLTLGTAANEGGTAKWSYHSVSFRDDNEQYNHYT
                     NGQWQGTVGLVRQDMVTYAK"
     gene            complement(2991..3335)
                     /locus_tag="VIN75_26345"
     CDS             complement(2991..3335)
                     /locus_tag="VIN75_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY8977762.1"
                     /translation="MQTTLPLRQIDHDVFVAPQLDAAAMAEVARLGFKSVVNNRPDFE
                     HGPDQPASAQVEAAALAAGLQYRHLPVAGGYQSPEEVAAFRQLLAELPHPLLAFCRSG
                     ARSTRLYQLAQG"
     gene            complement(3393..3839)
                     /locus_tag="VIN75_26350"
     CDS             complement(3393..3839)
                     /locus_tag="VIN75_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8977763.1"
                     /translation="MSLKQRISDDMKAAMRAKEADRLLAIRNLMAAMKQREVDERIEL
                     TDADVVAIVDKLVKQRKDSIDAFSKAGRTDLLEKEQAELAVLQGYLPERLDAAAIQAE
                     VNAIVAELGAAGPGDMGKVMGAVKARLAGKADMGQVSAAVKAALSK"
     gene            complement(3997..4209)
                     /gene="rpsU"
                     /locus_tag="VIN75_26355"
     CDS             complement(3997..4209)
                     /gene="rpsU"
                     /locus_tag="VIN75_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8977764.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTELRAREFYEKPTAE
                     RKRKKAAAVKRHYKRVRSMQLPKKLY"
BASE COUNT          624 a         1547 c         1678 g          740 t
ORIGIN      
        1 gatgtagcgc gggccgtagg gccggaacca cacgccgccg tggaagaagt agttgccgcc
       61 gccgtagccc acgctcacgg cgccgaccgg cagcgtgccg tacacgaagc cgcgcgacgg
      121 gtagtagtgg ttgtggccgt agcgggcgtc gaagtcgacg tagccgcgcg catgggcgcc
      181 gctccagccc gggccggcgc gacccggcgc ggggcccggc ttgcccggtg cgccgtgcgc
      241 gggcggcggg ccgccgcggt cgtggtcatg gtcgtggcct tgcgcctgcg cgcccgcgca
      301 cgcgagcgcc aggatcgtgg cggcgaaaag ggaatggcgg agtgtcatga cgttgtcgag
      361 ggccgcggcg catcccgcgc gcccattgtc gagaagccgt gtcagctgcc gattgcacca
      421 tgtcaacgcc atgtgaagcg gcgttgccgg caaggccgac acgtcctcag ggcctggcca
      481 ccggcccctg caactgcgcc agctgctgct gcgcctgcgt cacgtccttg ccgtattcgg
      541 cgaggtagcg ccgcgtctcg tcgctggaga acggcgcgtc gctgaactgg gccgccgcga
      601 ccttgcgcga ggtcgcgacg gggccgtcga gcgagggcat cagcgcgcgg tccgggtagg
      661 cgtcgggcct gagcaggaag ccctcgacgt cgtgcagcgt cagcggcatc gccggcttca
      721 ggtcgcgcac gaggcggccg tagacctgca acggcacctg gcccccgccc gcgcgcagtt
      781 cgtcgttgaa ggtgacgagc gccaccggcc tgccgttcgc gtcgtccacg cggccggtga
      841 tgacgtagcg tccgggctgg ttcacgtcga tgcccaggag gaacgccagc gagccgtcgc
      901 gcatctcgtc gtgcacgctg cccgtccagg tcgccggcgc ctgcggcgca tagacgacgt
      961 cgaacgcgac gtagccctgc tggtcgcccg actgcaccac cagctcggtg cggatcagtc
     1021 cggcgaagtt gccgaagccc tcggcgccgg gatcgagccg cgcggtgaag gtgccgtcgc
     1081 cggcgtgcgc gtcgccgttg gagccgtcgt cgacgaaggg ctgggtgacg accggcgcga
     1141 gcggcgccgc cttgccgccg ccggtgtcct gcgggctgtg cgccatcgag ctgaggacgc
     1201 gcagcggcag cacggcgccg ttgtcgtcgg tggccgtgac ggtcagcagc accgtgtcgg
     1261 cgccggtggc aaagaccttc tgctgcgtcg tgtgcacgtg caggttgggc acgacgccgg
     1321 tgcccggcat gcgcagcggc gcgtcggtgg tgaccacctg gtgggtctgc cactggtcgg
     1381 cgtgctcgct ggccgggcgc gagtcgaacg ggtagcgcgt cgcggcctgg tacatcgcca
     1441 gcgtctgctt cgcgcgatcc aggcgctgct gccacagcgc gagccgcgcc gcgcgatcgt
     1501 ccaggccggc cgcgctgaac ggcgcgcgcc gcggcgcgga cgcggccggc gcgacgtccc
     1561 gcggcgcggg cgcgccggcc gccgcctgcg cgaccggatc gagcacctgc gccggcggcg
     1621 tgctcgccgg cgacgccatc caccacagcc ccgcggtgcc gaccgcagcc acgggcacga
     1681 cgacgcgcca gcggatcttc atggccgcct cgccgcgcta cttggcgtag gtcaccatgt
     1741 cctggcgcac caggcccacc gtgccctgcc actggccgtt ggtgtagtgg ttgtactgct
     1801 cgttgtcgtc gcggaacgag accgagtggt agctccactt ggcggtgccg ccctcgttgg
     1861 cggcggtgcc cagcgtgagg tcattgcaga accagtcgct cgggttgcag tacgcgccgc
     1921 ccgagctgcc cgagacggcg cccgcgctgt ggtaggccac cgcctcgtcg tcctggcccg
     1981 gcagcaccgc ggaatagagc gtgcccttgg cgccggccca catgtagaac cagtggctgc
     2041 gcgtgttgtt gtggtcgtac atcgcgcggg cggtgccggt cttcaggtcc gagaccagcg
     2101 gctccgacgt cgcccagtcg cccacgttgg ccagctccga gccgccgccc gcgccggccg
     2161 cgacgtccac ccacttgatg ttccagccgg tctgcgtgcc cgcgccggac gcgccgcaga
     2221 cgccgctcga gttgggcgtg gcgttggtga tggtgcgcga cgagccgccg tacatcgcca
     2281 gcgtgtagcc gatcatcagg tcgcccgcgc tgtgcgccgc cacgtagcac cagttcgagc
     2341 ccgtgcagaa gcagtcgagc gcgttgcgca cgaggtagtt ctggtcggag atgtggttgt
     2401 agccgtccca gttgaccgac ttcttgttga cgcccgcggc cgtcgtgccc gggccccagt
     2461 aggtgaagtc cgcgtagttg cccgcctggc cgccgcccgt gcggccattg acccacagcg
     2521 aatagttggt cgcctgcgag actgcggcca agcccgcggc ggccaccaag gcggcgcaga
     2581 tcgagacgtt gcggagacgg acgtgcatgg gatcaccttt cgtgtgccga gaagacggag
     2641 ggcgaaatga cgtgtccgca atgtgcgcaa tatcgcgctg catcagcaat gcaggttttc
     2701 ccgcaacatg aaaaatggcc cgctaacccg catggatact gggttgcctg acgcagattg
     2761 cgtttgcgtc agcctgctta ccgatacgaa cgttcgtgcg cttgcgcggc aggccgcggg
     2821 catcggggtt gtgcgctcag ccgtttccca acgtctggcg cgaaggcttg tcttggcggg
     2881 cggcacgccg ccttcgaggc cggcgcgcag tggcgtcgcg gcaggggtgc aatcctgcga
     2941 ctgcgcttcg ctccgcgcag gatgaccgcg tgtcgggatc cgcgcggggc tcagccctgc
     3001 gcgagctggt acagccgcgt cgagcgcgcg cccgagcggc agaacgccag cagcggatgc
     3061 ggcagctcgg cgagcagctg gcggaaggcg gccacttcct cgggcgactg gtagccgccc
     3121 gccacgggca ggtggcggta ctgcaggccg gcggcgagcg cggcggcctc gacctgcgcc
     3181 gaggcgggct ggtcggggcc gtgctcgaag tcgggacggt tgttgacgac gctcttgaag
     3241 cccaggcgcg cgacttccgc catggcggcg gcgtcgagct gcggggcgac gaacacgtcg
     3301 tggtcgatct ggcgcagggg cagggtggtt tgcatcgagt gggatccttg atcggtgacg
     3361 tggctcgggg ccattgtggc cccggcctca gctcacttgg acagcgcggc cttcacggcg
     3421 gcggagacct ggcccatgtc ggccttgccg gcgagcctgg ccttgaccgc gcccatcacc
     3481 ttgcccatgt cgcccgggcc ggcggcgccc agctcggcca cgatggcgtt cacctcggcc
     3541 tggatcgccg cggcgtccag gcgctcgggc aggtagccct gcagcacggc gagctcggcc
     3601 tgttccttct cgagcaggtc ggtgcggccg gccttggaga acgcgtcgat cgaatccttg
     3661 cgctgcttga cgagcttgtc gacgatggcg acgacgtcgg catcggtcag ctcgatgcgc
     3721 tcgtccacct cgcgctgctt catcgcggcc atcaggttgc ggatcgcgag caggcgatcg
     3781 gcttccttgg cgcgcatggc ggccttcatg tcgtcggaga tgcgttgctt caggctcatg
     3841 gtcttggctt tcggtttgtt cttgtcgaca gatgggggcg tgcgcaaaag acaaaagccc
     3901 gctacggcgt cccgcggcgg gctttcgact tccgatgggt cgccgcgagc ggcgactatc
     3961 cggaggaaac gagctcgaga gctcccgtga aactcttcag tagagcttct tgggcaactg
     4021 catgctgcga acgcgcttgt agtggcgctt gacggcggcg gccttcttgc gcttgcgctc
     4081 ggcggtcggc ttctcgtaga actcgcgggc gcgcagttcg gtcagcagac ccagcttttc
     4141 gatggtgcgc ttgaagcggc gcagggccac gtcgaacggc tcgttttcct tgacacggat
     4201 ggtggtcatg tagcgaagaa tccttcggga gaaatatgcg ctcggagtcc ttgtccagac
     4261 ccgttgcgcc cgctcacttg cgtgggaaga gcgccgcggt ttctagaacg ccggacaaag
     4321 atcgacacgc ggtttgccag caaagccaag gattctaccc cgaaatgcct ggatgtgcga
     4381 atttttatgc ggcggcgcac caacgggccg cgattcggcg cgatcgcggg cgcattcagc
     4441 gcggctgcag cgccaggtcg gcgacgacca cggcgacgcg cgggtcgggc gccggctgcg
     4501 gccgccgcac gacgatcgtg acgcgctccc aggcggggtc ggccagcagc tcgcgcagca
     4561 ccaggccgcc gacgaggccg gtggcgccg
//
LOCUS       DATCRO010000963         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_876993, whole genome shotgun sequence.
ACCESSION   DATCRO010000963 DATCRO010000000
VERSION     DATCRO010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876993"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIN75_26360"
     CDS             <1..247
                     /locus_tag="VIN75_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY8977765.1"
                     /translation="IDLEVRAFAEPIGVQEDPVTGSLNASLAEWLIADGHMPERYLAA
                     QGQCLGRAGRVHIERDAEGRIWVGGDAVTCVDGSVSL"
     gene            262..954
                     /locus_tag="VIN75_26365"
     CDS             262..954
                     /locus_tag="VIN75_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8977766.1"
                     /translation="MHAQLDHLVIAAASLAEGVAWCEATLGITPAPGGSHPLMGTHNR
                     LFNVSGDAYPAAYAEIIAIEPGRKPSRPKTRRWFDMDDAALQAGLVRHGPRLIHVVAR
                     VPDAHAAMRALAHEEHAHIDRGQLLEASRDTPAGRLEWQITVRDDGQRLFYGALPTLI
                     QWGAVHPTDAMPASGVVLRSLRATHPRAPALAAALSAIGMTGLEVDAGPPNLVAVLDT
                     PRGSVTLESQGL"
     gene            956..1588
                     /locus_tag="VIN75_26370"
     CDS             956..1588
                     /locus_tag="VIN75_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8977767.1"
                     /translation="MLSFTEIALFAFASLLLALTPGPNMIYCVSRTLVQGRRAGLISL
                     GGVLLAFLVHLFAAALGLTALLLAVPLAFDAIRIAGAAYLLWLAWQAVKPGGTAPFEA
                     RALPADPPGKLFRMGFLTNLLNPKVAMFYLSFFPQFIHPERGSVLLQSMQLGIAQMTA
                     SALVNTVMIVGAAGITAVLSQSAGWLRAQRYVMGSVLAALAVRVALTERK"
     gene            1612..2577
                     /locus_tag="VIN75_26375"
     CDS             1612..2577
                     /locus_tag="VIN75_26375"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEY8977768.1"
                     /translation="MRFTREEIESAQRTVRAAMPPTPQYAWPLLSQRLGATVWAKHEN
                     HTPAGAFKIRGGLTYFETLAREQPTVRHAISATRGNHGQSVGFAARRHGLGATIVVPH
                     GNSTEKNAAMRALGVTLVEHGDDFQAASEHAAALAERDGLHRVPSFHRELVRGVSTAY
                     VEFFEALSATPPDVLFVPIGLGSGFAAAAAARAHCGVSTKLIGVVSAHATAYRDSFRA
                     GKPVESPVTTRLADGMACRTPVPESVEVMLREADDVVTVTDDEIAQSMRILFSDTHNV
                     AEGAGAAALAAALQQQERWRGRTVGVCLSGGNVDSDMFAGVLAAA"
     gene            complement(2602..>3063)
                     /locus_tag="VIN75_26380"
     CDS             complement(2602..>3063)
                     /locus_tag="VIN75_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY8977769.1"
                     /translation="HGGHVGAMDIVARELRAEHGLIVYSVSWFNLPLGDAGAQFSAQE
                     HRFGVHAGEIETSMMLALSPQLVRMGEARDFDSTSRQRAADYAILGNGRSAKLGWAIE
                     DYNAHGAAGNAAAATAAHGQAVVDEAARQLALLLEEVSRLPLETANTGPLP"
BASE COUNT          421 a         1128 c         1070 g          444 t
ORIGIN      
        1 gatcgacctc gaggtgcgcg ccttcgccga gcccatcggc gtgcaggaag acccggtcac
       61 cggcagcctc aacgcgagcc tggccgaatg gctgatcgcc gacggccaca tgcccgagcg
      121 ctaccttgcc gcccaaggcc agtgcctggg ccgcgcgggc cgcgtccaca tcgagcgcga
      181 cgccgaaggc cgcatctggg tcggcggcga cgccgtgacc tgcgtggacg gcagcgtctc
      241 gctctgaacg ggccgcccgc catgcacgcg caactcgacc acctggtgat cgccgccgcc
      301 tcgctcgccg agggcgtggc gtggtgcgag gccacgctcg gcatcacgcc ggcaccgggc
      361 ggctcgcatc cgctcatggg cacgcacaac cggctgttca acgtctccgg cgacgcctat
      421 ccggcggcct atgccgagat catcgcgatc gagccgggca ggaagccgtc gcggcccaag
      481 acgcgccgct ggttcgacat ggacgatgcc gcgctgcagg ccggcctcgt gcggcatggc
      541 ccgcggctga tccacgtcgt cgcgcgcgtg cccgatgcgc atgcggcgat gcgcgcgctg
      601 gcgcatgaag agcacgcgca catcgaccgc ggccagctgc tcgaagcctc gcgcgacacg
      661 cccgccggcc ggctcgaatg gcagatcacc gtgcgcgacg acggccagcg gcttttctat
      721 ggcgcgctgc ccacgctgat ccagtggggc gcggtgcatc ccaccgacgc catgcccgcg
      781 tcgggcgtcg tgctgcgctc gctgcgcgcc acgcacccgc gcgcgccggc gctggccgcc
      841 gcgctgtcgg ccatcggcat gacgggcctc gaggtcgatg ccggcccgcc caacctggtt
      901 gccgtgctcg acacgccgcg cggcagcgtg accctcgaat cacaaggact ctgacatgct
      961 gagcttcacc gagatcgccc tcttcgcatt cgcctcgctg ctgctggcgc tcacgcccgg
     1021 gccgaacatg atctattgcg tctcgcgcac gctggtgcag ggccggcgcg cggggctcat
     1081 atcgctgggc ggcgtgctgc tggccttcct ggtgcacctg ttcgcggcgg cgctcgggct
     1141 caccgcgctg ctgctggcgg tgccgctcgc gttcgacgcg atccgcatcg ccggcgcggc
     1201 ctacctgctg tggctcgcat ggcaggcggt gaagcccggc ggcaccgcgc ccttcgaggc
     1261 gcgcgcgctg ccggccgacc cgccgggcaa gctgttccgc atgggctttc tcacgaacct
     1321 gctgaacccg aaggtggcga tgttctatct ctcgttcttc ccgcagttca tccaccccga
     1381 gcgcggctcg gtgctgctgc agagcatgca gctgggcatc gcgcagatga ccgccagcgc
     1441 gctggtcaac acggtgatga tcgtcggcgc tgcaggcatc acggccgtgc tgtcgcaaag
     1501 cgccggctgg ctgcgcgcgc agcgctacgt gatgggcagc gtgctcgccg cgctggccgt
     1561 gcgggtggcg ctgaccgagc gcaagtagga aggcaggaag agagacaccc catgagattc
     1621 acccgcgaag agatcgaatc ggcgcagcgc accgtgcgcg cggccatgcc gcccacgccg
     1681 cagtacgcct ggccgctcct gtcgcagcgc ctgggcgcca ccgtgtgggc caagcacgag
     1741 aaccacacgc cggccggcgc cttcaagatc cgcggcggcc tgacctactt cgagacgctg
     1801 gcgcgcgagc agccgacggt gcgccacgcg atcagcgcca cgcgcggcaa ccacggccag
     1861 tcggtcggct tcgcggcacg gcgccacggg ctgggtgcga ccatcgtcgt gccgcacggc
     1921 aactcgaccg agaagaacgc cgcgatgcgt gcactgggcg tcacgctggt cgagcacggc
     1981 gacgacttcc aggccgcgtc ggaacatgcg gccgcgctcg ccgaacgcga cggcctgcac
     2041 cgcgtgccct cgttccaccg cgagctggtg cgcggcgtct cgacggcgta tgtcgagttc
     2101 ttcgaagcgc tctcggccac gccgcccgac gtgcttttcg tgcccatcgg cctgggctcg
     2161 ggcttcgcgg ccgcagcggc ggcgcgcgcg cattgcggcg tgtcgacgaa gctcatcggc
     2221 gtggtgtcgg cgcatgcgac ggcctatcgc gattccttcc gcgcgggcaa gccggtcgaa
     2281 tcgcccgtga ccacgcggct ggccgacggc atggcctgcc gcacgccggt gcccgaatcg
     2341 gtggaggtga tgttgcgaga ggccgacgac gtggtcaccg tcaccgacga cgagatcgcg
     2401 cagtccatgc gcatcctttt cagcgacacg cacaacgtgg ccgaaggggc cggcgcggcc
     2461 gcgctggccg cggcgctcca gcagcaggag cgctggcgcg gccggacggt gggcgtatgc
     2521 ctgagcggcg gcaatgtcga ctcggacatg ttcgccggcg tgctggccgc cgcctgaccg
     2581 gaactcagga agctcgggag ttcagggcag aggcccggta ttggccgttt ccagcggcag
     2641 ccgcgacacc tcttccagca gcagcgcgag ctgccgtgcg gcctcgtcga ccaccgcctg
     2701 cccgtgcgcc gcggtggcgg cggccgcgtt gcccgcggcg ccgtgggcgt tgtagtcctc
     2761 gatggcccag ccgagcttgg cgctcctgcc gttgcccagg atcgcgtagt cggcggcgcg
     2821 ctggcgcgag gtggaatcga aatcccgcgc ctcgcccatg cgcaccaact gcggtgagag
     2881 cgccagcatc atcgaagtct cgatctcgcc cgcgtgcacg ccgaagcggt gctcctgcgc
     2941 gctgaactgc gcgcccgcgt cgcccagcgg caggttgaac cagctcacgc tgtagacgat
     3001 gagcccgtgc tcggcgcgca gctcgcgcgc cacgatgtcc atcgccccca cgtggccgcc
     3061 atg
//
LOCUS       DATCRO010000964         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1012360, whole genome shotgun sequence.
ACCESSION   DATCRO010000964 DATCRO010000000
VERSION     DATCRO010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012360"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VIN75_26385"
     CDS             complement(<1..1756)
                     /locus_tag="VIN75_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY8977770.1"
                     /translation="MKRMLINATQPEERRLAIVDGQKLLDFENEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISRQYFRDGVDVKSARIQDAIKDGQELLVQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEDREELKENLDQLEYP
                     KGMSLIARTAGIGRSAPELQWDLNYMLKLWDAIDGASKSGKGAFLIYQESSLVIRAIR
                     DYFTADIGEILIDTDDIYEQAQQFMNHVMPESASRVKRYRDDAALFSRFQIEHQIETA
                     FSRTVNLPAGGAIVIDHTEALVSIDVNSARATRGGDIEETATRTNIEAADEIARQMRL
                     RDLGGLIVVDFIDMEESKNRREVEQRLRDALRFDRARVQFSSISKFGLLELSRQRLRP
                     ALSEGSHITCPRCNGTGHIRDTESSALQILRIIQEESMKENTAAVHVQVPVEVTSFLL
                     NEKRSEITKIELKQRTTVLLVPNKHLETPNYKLERLRHDDARLENLQASYTMIEEPQD
                     EVSITRRDKAKAKQEPVIKGVLPDQPAPPAPAPAPAPVAATPAPAPRPAAAPVPAAAS
                     GGFFGWITSLFGAPAPAPA"
     gene            2728..3678
                     /locus_tag="VIN75_26390"
     CDS             2728..3678
                     /locus_tag="VIN75_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8977771.1"
                     /translation="MRNVAVDAEFEGQRLDNFLLRELKGVPKTHVYRIIRSGEVRVNK
                     GRASADTKLAAGDVVRVPPVRTATPATPAEAAPPREFPILFEDEHLLAIDKPAGTAVH
                     GGSGVSFGVIEQLRRARPQQKYLELVHRLDRETSGILLLAKKRSALVALQDQFRARET
                     GKTYAALVTGRWPARLKVIDVALHKTQDAAGERRVHPVADDHPDGQRSISLVRIARQF
                     ADTTLLDVTIKTGRTHQIRVHLQHSGHVIVGDDRYGDFALNRRFARGEAVPGVRFERM
                     FLHARRLAFDHPASGQRLELEAPLPPECAALVDALARSSP"
     gene            3675..>4029
                     /locus_tag="VIN75_26395"
     CDS             3675..>4029
                     /locus_tag="VIN75_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY8977772.1"
                     /translation="MTDSPDRPRRFDLVVFDWDGTLFDSTALIVHSIQAACADLGLPV
                     PDDERASWVIGLSLRAAMAHAAPTLTEAQLPAMIDRYRFHYLARQHDLSLFPGVLDML
                     RALKRRHHWLAVATGK"
BASE COUNT          552 a         1407 c         1415 g          655 t
ORIGIN      
        1 gggcaggcgc gggcgcgggg gcgccgaaca ggctcgtgat ccagccgaag aacccgcccg
       61 aggccgctgc cggcacgggg gccgcggccg gacgcggggc cggggccggc gtggcggcca
      121 cgggagccgg cgcgggcgcc ggcgccgggg gcgcgggctg gtcgggcagc acgcccttga
      181 tgaccggctc ctgcttggcc ttggccttgt cgcggcgcgt gatcgagacc tcgtcctgcg
      241 gctcctcgat catcgtgtag ctcgcctgca ggttctccag gcgggcgtcg tcgtggcgca
      301 ggcgctcgag cttgtagttg ggcgtctcca ggtgcttgtt gggcaccagc agcacggtgg
      361 tgcgctgctt gagctcgatc ttggtgatct cgctgcgctt ctcgttgagc aggaaggacg
      421 tcacctccac cggcacctgc acgtgcacgg cggcggtgtt ctccttcatc gactcctcct
      481 ggatgatgcg caggatctgc agcgcggagg actcggtgtc gcggatgtgg cccgtgccgt
      541 tgcagcgcgg gcaggtgatg tggctgcctt cggacagggc cgggcgcagg cgctggcgac
      601 tcagctcgag cagcccgaac ttgctgatcg acgagaactg cacgcgggcg cggtcgaagc
      661 gcagcgcgtc gcgcaggcgc tgctcgacct cgcggcggtt cttcgactct tccatgtcga
      721 tgaagtcgac gacgatcagg ccgcccaggt cgcgcaggcg catctggcgg gcgatctcgt
      781 cggcggcctc gatgttggtg cgggtggcgg tctcctcgat gtcgccgccg cgcgtggcgc
      841 gcgccgagtt gacgtcgatc gagacgagcg cctcggtgtg gtcgatgacg atcgcgccgc
      901 cggccggcag gttgaccgtg cgcgagaacg cggtctcgat ctggtgctcg atctggaagc
      961 ggctgaacag cgccgcgtcg tcgcggtagc gcttcacgcg cgatgccgac tcgggcatca
     1021 cgtggttcat gaactgctgg gcctgctcgt agatgtcgtc ggtgtcgatc aggatctcgc
     1081 cgatgtccgc ggtgaagtag tcgcggatgg cgcggatgac cagcgacgat tcctggtaga
     1141 tcaggaacgc gcccttgccc gacttggagg cgccgtcgat ggcgtcccac agcttgagca
     1201 tgtagttcag gtcccactgc agctcgggcg cgctgcggcc gatgccggcg gtgcgcgcga
     1261 tcaggctcat gcccttgggg tactcgagct ggtcgaggtt ctccttgagt tcctcgcggt
     1321 cctcgccctc gatgcgacgg ctgacgccgc cgccgcgcgg gttgttgggc atcagcacca
     1381 ggtagcggcc ggccagcgag atgaacgtgg tcagggccgc gcccttgttg ccgcgctctt
     1441 ccttctcgac ctgcaccagc agttcctggc cgtccttgat ggcgtcctgg atgcgggcgg
     1501 acttgacgtc gacgccgtcg cggaaatact ggcgcgagat ttccttgaac ggcaggaaac
     1561 cgtggcgatc ctcgccgtag tcgacgaaac acgcctcgag cgacggctcg acgcgggtca
     1621 cgaccgcctt gtagatattg cccttgcgct gttcgcgccc ttcgatctcg ttctcgaaat
     1681 cgagcagttt ctgtccgtcg acgatcgcca gccggcgttc ttccggctgg gtcgcgttga
     1741 tcagcatgcg tttcacgtgc actccttcat cgcctgcgcg cggccgctgg ccgcgacgag
     1801 gctgtcgtct cactgcgcgt caccggggcc gtggtgggaa tggccccggc atcgcgccaa
     1861 cgatgcacga gaaacgaact gcgaaccgat ggaaggaacc gccctggact gcgccgccat
     1921 gccccgatca gggagcggat gacggcgcgg cgttggcgct ttggtgctca gtccctgcgt
     1981 cgcgttgcga gggctgtttc ccgagaagca cctgcggccg cggccgcggc cgctcctgca
     2041 ggtccgcgcg cccgaactgc ccgggtccgc ggcgacggca gggcggtggc gccggcgcgg
     2101 cgcgatcgtg cgccgggcag cggccagggc cgtgcttcgc ccgcggtgcg ggactgggtc
     2161 ggatgggggt gggaaacgaa cgccatgcga ttgcgaatgc aaaggatctg gcaccggctg
     2221 ccagctgcga tttccggctg ggaaaaagcc tctggcgacc ttgggaaacc tttatctcca
     2281 gacctgcaac caaggcaggt cggttcttgt gtcgttgctc ttccgcgtct ttgcattcga
     2341 ccgccgggcc acttcgtggt caccggcctg ccggggcgcc gagcgcgttg catcacctgt
     2401 tttgccgcgt ctctttcgat attcggcgcc acgcccgcgc acccttgttg tcgagtttga
     2461 aagtccgccg caggatcccg atcggggatt gcccgggtaa actctcgaaa atcaataact
     2521 tatggcgcaa acgtggttcg gccgattata gaggggaaac gccagccggc ccaaaccggc
     2581 gcccgcggcg ctcccaaggc gaaggcggcg gcgggccgcc aggccgttcc cgccgccggc
     2641 gccaaggcgc gcccgcgtcc ggctgcggcc accagccggc ccgcgcccgc cgcgcccgtc
     2701 gccgcgccgc ccgaggcggc cgcggtggtg cgcaacgtgg ccgtcgatgc ggaattcgag
     2761 ggccagcgcc tggacaactt cctgctgcgc gagctcaagg gcgtgcccaa gacgcacgtc
     2821 taccggatca tccggtccgg cgaggtgcgc gtgaacaagg gccgcgcctc ggccgacacc
     2881 aagctggccg ccggcgacgt cgtgcgcgtg ccgccggtgc gcacggccac gcccgcgacg
     2941 ccggccgagg ccgcgccgcc gcgcgaattc ccgatcctgt tcgaggacga gcacctgctg
     3001 gccatcgaca agcccgccgg caccgcggtg cacggcggct cgggcgtcag cttcggcgtc
     3061 atcgagcagc tgcgccgcgc gcgcccgcag cagaaatacc tcgagctcgt ccaccggctc
     3121 gaccgcgaga cctccggcat cctgctgctg gccaaaaagc gcagcgcgct ggtggcgctg
     3181 caggaccagt tccgcgcccg cgagacgggc aagacctacg ccgcgctcgt gacgggccgc
     3241 tggccggcca ggctcaaggt gatcgacgtc gcgctgcaca agacgcagga cgccgccggc
     3301 gagcgccgcg tccacccggt ggccgacgac caccccgacg gccagcgctc gatctcgctg
     3361 gtgcgcatcg cccggcagtt cgccgacacc acgctgctgg acgtcaccat caagacgggc
     3421 cgcacgcacc agatccgcgt ccacctgcag cattccgggc acgtgatcgt cggcgacgac
     3481 cgctacggcg acttcgccct caaccgccgc ttcgcgcgcg gcgaggcggt gccgggggtg
     3541 cgcttcgagc gcatgttcct gcacgcgcgc cggctcgcct tcgaccatcc cgccagcggg
     3601 cagcgcctcg agctggaggc gccgctgccg ccggagtgcg ccgccctcgt cgatgccctg
     3661 gcaaggtcgt caccatgacc gattcccccg accgcccgcg ccgcttcgac ctggtcgtct
     3721 tcgactggga cggcacgctg ttcgactcca cggcgctgat cgtgcacagc atccaggccg
     3781 cgtgcgccga cctcgggctg ccggtgcccg acgacgaacg cgcctcgtgg gtgatcggcc
     3841 tgagcctgcg cgccgcgatg gcgcatgcgg cgcccacgct cacggaggcg cagctgccgg
     3901 cgatgataga ccgctaccgc ttccactacc tcgcgcgcca gcacgacctg tcgctgttcc
     3961 cgggcgtgct ggacatgctg cgcgcgctca agcgccgcca ccactggctg gcggtggcca
     4021 ccggcaaga
//
LOCUS       DATCRO010000965         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_449641, whole genome shotgun sequence.
ACCESSION   DATCRO010000965 DATCRO010000000
VERSION     DATCRO010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449641"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..2230
                     /locus_tag="VIN75_26400"
     CDS             128..2230
                     /locus_tag="VIN75_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8977773.1"
                     /translation="MAQAPAASAPASAAIASADDPNLWLEDILGDKALAWVRERNAQS
                     RKTLEAWPQFQQTRDQLRAVLDSKDQIPYVTRRGASLWNFWRDDKNPRGLWRRTTLDE
                     YRKPQPSWDVVIDLDELAKKEGENWVWHGAACLAPRYERCLVMLSRGGSDAQVVREYD
                     IARRAFVTDGFVLPEAKSSVEWADENTIYVATDFGPGSLTDSGYPNVVKRWSRGTPLA
                     SAATMFTGEHTDVASGMSVDDTPGFERTTFSRSLDFYTSRVWLLDKLDPAGKSPELTL
                     IDKPDDANLSFWKSRVLIELRSDWKLGDTTWPRGSLLVGDAKAYLAGERHLQALFTPT
                     ATRSLAGFSTTRSRILLNVLDNVTGRIEELTPTATGWERHAVQAPALGTLSAYGIDDP
                     HVADDPYAEAYFLNYTDFLTPDSLYLGHTGSDKRELLKSRPHFFDERGMHVEQKFATS
                     RDGTKVPYFIIWPRGARADGANPTLLYGYGGFEISLTPSYSGGIGRAWYEHGGVWVYA
                     NIRGGGEYGPGWHQAAIRENKQRSYDDFIAVAEDLVATRVTSPRHLGIEGGSNGGLLV
                     GAVTMQRPDLFNAVSCQVPLLDMRRYNKLLAGASWMAEYGNPDIPAEWAWISKYSPYQ
                     NVHKDTRYPTVFFTTSTRDDRVHPGHASKMAARMIEQGHDVLYYENIEGGHGGAADNA
                     QRADVQALQFAFLWQKLK"
BASE COUNT          393 a          858 c          750 g          312 t
ORIGIN      
        1 gaatggcccc aggcgcgtcc cggctcacga ggccggggcg ctttcctcat ccgatccctc
       61 tgcgatgatc aagacctccc gactcctgtg cgtggccgcc gcgctggcga ccgccgcaag
      121 ctccctcatg gcccaagccc ccgctgcctc cgcccccgca tccgccgcca tcgcgagcgc
      181 ggacgacccg aacctgtggc tggaggacat cctcggcgac aaggccctcg cttgggtgcg
      241 cgagcgcaac gcgcagtcgc gcaagacgct cgaagcctgg ccgcagttcc agcagacgcg
      301 cgaccagctg cgcgccgtcc tcgattcgaa ggaccagatc ccctatgtca cgcgccgtgg
      361 cgcatccctc tggaacttct ggcgcgacga caagaacccg cgcggcctgt ggcgccgcac
      421 cacgctggac gaataccgca agccgcagcc cagctgggac gtcgtgatcg acctcgacga
      481 gctcgcgaag aaggaaggcg agaactgggt ctggcacggc gccgcctgcc tggcgcccag
      541 gtacgagcgc tgcctcgtga tgctgtcgcg cggcggctcg gacgcccagg tcgtgcgcga
      601 gtacgacatc gccaggcgcg cgttcgtcac cgacggcttc gtgctgcctg aggccaagtc
      661 cagcgtcgaa tgggccgacg agaacacgat ctacgtggcc accgacttcg gccccggcag
      721 cctgaccgac tccggctacc cgaacgtcgt caagcgctgg tcgcgcggca cgccgctggc
      781 cagcgccgcc accatgttca ccggcgagca caccgatgtc gcgtcgggca tgagcgtcga
      841 cgacacgccc ggcttcgagc gcaccacctt cagccgttcg ctcgacttct acacctcgcg
      901 cgtctggctg ctcgacaagc tcgaccccgc cggcaagtcg cccgagctga cgctgatcga
      961 caagcccgac gacgccaacc tcagcttctg gaagagccgc gtgctgatcg agctgcgctc
     1021 cgactggaag ctcggcgaca cgacgtggcc gcgcggctcg ctgctggtgg gcgacgccaa
     1081 ggcctatctc gcgggcgagc gccacctgca ggcgctgttc acgccgacgg ccacgcgttc
     1141 gctggccggc ttcagcacga ccaggtccag gatcctgctg aacgtcctcg acaacgtgac
     1201 cggccgcatc gaggagctca cgccgacggc caccggctgg gaacgccacg cggtgcaggc
     1261 gcccgcgctc ggcacgctgt ccgcgtacgg catcgacgac ccgcacgtgg ccgacgaccc
     1321 gtacgccgag gcctacttcc tcaactacac cgacttcctc acgccggact cgctgtacct
     1381 ggggcacacg ggcagcgaca agcgcgagct gctgaagtcg cggccgcact tcttcgacga
     1441 gcggggcatg catgtcgagc agaagttcgc gacgtccagg gacggcacca aggtgcccta
     1501 cttcatcatc tggcccaggg gcgcgagggc cgacggcgcc aacccgacgc tgctgtacgg
     1561 gtacggcggc ttcgagatct cgctgacgcc cagctactcg ggcggcatcg gcagggcctg
     1621 gtacgagcac ggcggcgtct gggtctacgc caacatccgc ggcggcggcg agtacgggcc
     1681 gggctggcac caggccgcga tccgcgagaa caagcagcgc agctacgacg acttcatcgc
     1741 ggtggccgag gacctggtcg ccacccgggt gaccagcccc cgccacctcg gcatcgaagg
     1801 cggcagcaac ggcggcctgc tggtgggcgc cgtcacgatg cagcgccccg acctgttcaa
     1861 cgccgtcagc tgccaggtgc cgctgctgga catgcgccgc tacaacaagc tgctggccgg
     1921 cgcgtcgtgg atggccgagt acggcaaccc ggacatcccg gccgagtggg cctggatctc
     1981 caagtacagc ccgtaccaga acgtccacaa ggacaccagg tacccgacgg tgttcttcac
     2041 cacgtccacg cgcgacgacc gcgtgcaccc gggacacgcg agcaagatgg ccgcgcgcat
     2101 gatcgagcag ggccacgacg tgctgtacta cgagaacatc gagggcggcc acggcggcgc
     2161 ggcggacaac gcgcagcgcg cggacgtcca ggcgctgcag ttcgccttcc tctggcaaaa
     2221 gctgaagtga tcgtgcacgg gtggcgcgca gcttgccgct gcagcgtgcc accgcccccg
     2281 gagccctcga tgaccgtcgt cgtcacccac aac
//
LOCUS       DATCRO010000966         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_607278, whole genome shotgun sequence.
ACCESSION   DATCRO010000966 DATCRO010000000
VERSION     DATCRO010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607278"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..391)
                     /locus_tag="VIN75_26405"
     CDS             complement(200..391)
                     /locus_tag="VIN75_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977774.1"
                     /translation="MTKTTAQAAAFALSALMTFGVVAGMNGIATKQYAAAERVAQVAA
                     DATQVAALQRVVVVAHRAA"
     gene            complement(864..1055)
                     /locus_tag="VIN75_26410"
     CDS             complement(864..1055)
                     /locus_tag="VIN75_26410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977775.1"
                     /translation="MTKTTAPFAAFALSALVTFGIVAGMNGIASKQYATAERVAQVAA
                     DATQVAALQRVVVVARRAA"
     gene            complement(1197..2132)
                     /locus_tag="VIN75_26415"
     CDS             complement(1197..2132)
                     /locus_tag="VIN75_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977776.1"
                     /translation="MSNPASTADRFELMETFVRIVDAGSLSAAAAQLGTTQPTVSRRL
                     QALERSLGVRLLRRSTHSMTLTEDGQRCLERARELLADWHAFEADLRGAGDEPEGTLR
                     VIAPHAFGQQQLVTPLAEYLARYPKVNVEWLLHDRRPDFITEGIDCAIQVGSIDDQAA
                     VAIRIAEVPRIVVGTPSLADALPARQSPADLAQLPWLAMRPYYRTEVALTSLDGGGDA
                     RFAIAPRMSTDSLYALRTATLMGVGASIVSAWLVADDLAQGRLVHLAPQWRAAPLPVW
                     LVYPQARFYPARLRRFLDVIRTHAAHALAPRPPGG"
     gene            2225..>3017
                     /locus_tag="VIN75_26420"
     CDS             2225..>3017
                     /locus_tag="VIN75_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977777.1"
                     /translation="MSSVLPSQLPTAAPARPLTPSLVGLLAAGAGLSVAALYYSQPML
                     GELQADIGAGAREVGLVPTLTQLGYAAGIFFLAPLGDRYDRRRIILVKAAALVVALLV
                     AGASSGMPLLLAASLAIGLAATLAQDIVPAAATLAPDSQRGRIVGTVMTGLLMGILLS
                     RVVSGVVASHFGWRAMFVAAAVSIAGFAAVAARGLPRFRPTTHLHYGALLASAAQLWK
                     RHPALRRAALAQGLLSIGFSAFWSTLAVMLHGSSWHLGSAAAGAFG"
BASE COUNT          404 a         1046 c         1127 g          440 t
ORIGIN      
        1 gtcgtagggc ttgtcggcgg cggcggccag gccggcgcac agcgacaagg cgatggagag
       61 cagcaatttc ttcatgccgc catgatgcca gcgtcgtgcc ggcggcgtcg gccggcggcc
      121 gggcgtcccc gcacgcatgt cgggcgcggg aatggaaaag ccccgccgaa gcggggctcg
      181 aaggagcggg aagagcggat caggcggcgc gatgcgcgac caccaccacg cgttgcagcg
      241 cggcgacctg cgtcgcatcg gccgcgacct gggccacgcg ctcggcggcc gcgtactgct
      301 tcgtggcgat gccgttcatg ccggcgacga cgccgaaggt catgagggcg gacagggcga
      361 aggcggcagc ttgggcggtg gtcttggtca tgatgggctc cttggggtgg ttggttcgga
      421 accgcgtttc gtgttccgat gggtgaacta tcggagttcg cggatcgagc cgccagcgac
      481 ttgcgacgag ccggcccgtg cgcggcacca gccgtcgtgg cggccgacgg accgtcagcg
      541 caagcggggt caggtcttga atcgcgcaag cgggggtccg caagcgcaag cgggggtcag
      601 gtcttgaatg tcgcggggtc aggtcttgaa tcgtgcactc gtccggacgg tccctcgggc
      661 cgccaagtgt ggcgatacgt acgcgagact ccggcgggca ggcttcagcg cttgctaggc
      721 aagacctggc accccgaatg gcacatcgga gcgcatgatt caagacctga ccccgccttg
      781 tgccgacgtc gcctgacgcc gcgcccggcg cggcaacaaa aaagccccgc cgaagcgggg
      841 ctcgaaggag cggtgcgtgc cggtcaggcg gcgcggcggg cgaccacgac cacgcgctgc
      901 agcgcggcga cctgcgtcgc atcggccgcg acctgggcca cgcgctccgc ggtcgcatat
      961 tgcttcgagg cgatgccgtt catgccggcg acgatgccga aggtcacgag ggcggacagc
     1021 gcgaaggcgg cgaacggggc ggtggtcttg gtcatggtgg gctcctgggg gtgttggttc
     1081 gaatcgcgtc tcgtgatccg atggatgaac tgtcggagct tcgaacccgc cggccaaccg
     1141 gcttgcgacg agccggcccg ttggcggcgc cagccgtcgt ttcgcgcgac ggaccgtcag
     1201 ccgcccggcg gcctgggcgc caacgcatgg gccgcgtgag tgcggatcac gtccaggaag
     1261 cggcgcagcc gcgccgggta gaagcgcgcc tgcggataga ccagccacac cggcagcggg
     1321 gccgcgcgcc attgcggcgc caggtggacc agccggccct gcgcgaggtc gtcggccacc
     1381 agccaggccg acacgatgga cgcgcccacg cccatcagcg tggcggtgcg cagcgcgtag
     1441 agactgtcgg tgctcatgcg cggtgcgatc gcaaagcgcg cgtcgccgcc tccatcgagc
     1501 gacgtcagcg ccacctcggt gcggtagtac ggccgcatcg cgagccaggg cagctgcgcg
     1561 aggtcggcgg gcgactggcg cgcgggcaac gcgtccgcga gcgacggtgt gcccacgacg
     1621 atgcgcggca cctcggcgat gcggatcgcc acggcggcct ggtcgtcgat gctgccgacc
     1681 tggatcgcgc agtcgatgcc ctcggtgatg aagtcgggcc ggcggtcgtg cagcagccat
     1741 tcgacgttga ccttgggata ccgcgcgagg tactcggcca gcggcgtgac gagctgctgc
     1801 tggccgaacg cgtgcggcgc gatgacgcgc agcgtcccct cgggctcgtc gccggcgccg
     1861 cgcaggtcgg cctcgaacgc gtgccagtcc gccagcagct cgcgcgcgcg ctcgaggcag
     1921 cgctggccgt cctcggtgag cgtcatcgag tgcgtcgagc ggcgcagcag ccgcacgccc
     1981 agcgagcgct cgagcgcctg cagccggcgg ctgacggtgg gctgcgtcgt ccccagttgc
     2041 gccgcggcgg ccgacaggct gccggcatcg acgatgcgca cgaaggtctc catcagctcg
     2101 aagcgatcgg ccgtggacgc ggggttgctc atgcgtcaag cgtatagcaa ttgttcaatc
     2161 gcggccacta cacaggtgtt ggatgcatcc ggagaatgac cctcattcac gaagggtccc
     2221 cgccatgtcg tccgtcctgc cctcccaact gccgaccgct gcgccggccc gtcccctgac
     2281 cccgtcgctc gtcggcctgc tcgccgcggg cgccggcctg tcggtcgccg cgctgtacta
     2341 cagccagccg atgctgggcg agttgcaggc cgacatcggc gccggcgcgc gcgaagtcgg
     2401 cctcgtgccc acgctcacgc agctgggcta tgccgccggc atcttcttcc tggcgccgct
     2461 gggcgaccgc tacgaccgcc gccgcatcat cctggtgaag gccgccgcgc tggtcgtggc
     2521 gctgctggtg gcgggcgcgt cgtcgggcat gccgctcctg ctggccgcga gcctggccat
     2581 cggcctggcc gcgaccctgg cgcaggacat cgtccccgcc gccgcgacgc tcgcgcccga
     2641 ctcgcagcgg ggccgcatcg tcggcaccgt gatgacgggc ctgctgatgg gcatcctgct
     2701 gtcgcgggtg gtcagcggcg tcgtggcctc gcacttcggc tggcgcgcga tgttcgtcgc
     2761 cgcggcggtc agcatcgccg gcttcgcggc cgtggccgcg cgcggactgc cgcgcttccg
     2821 cccgacgacg cacctgcact acggcgcgct gctggcctcg gcggcgcagc tgtggaagcg
     2881 ccacccggcg ctgcgtcgcg ccgcgcttgc gcaaggcctc ctgtcgatcg gcttcagcgc
     2941 gttctggtcg acgctcgcgg tgatgctgca cggatcgtcg tggcacctgg gcagcgccgc
     3001 ggccggcgcg ttcggcc
//
LOCUS       DATCRO010000967         5465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_888785, whole genome shotgun sequence.
ACCESSION   DATCRO010000967 DATCRO010000000
VERSION     DATCRO010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888785"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIN75_26425"
     CDS             complement(<1..194)
                     /locus_tag="VIN75_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY8977778.1"
                     /translation="MNNQWFSKIAVWLVIALVLFTVFKSFNTAQTQGNRVAYSDFLEE
                     VRNKHIQSVEITEGSGGIDIT"
     gene            complement(363..1028)
                     /locus_tag="VIN75_26430"
     CDS             complement(363..1028)
                     /locus_tag="VIN75_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY8977779.1"
                     /translation="MKINTKSKKVNKQWLQDHANDTYVKLAQKEGYRARAAYKLSEID
                     EQFKLIRPGQLVVDLGSTPGAWSQYIRRKFAGGGAAVGALNGRIVALDILEMEPIEGV
                     TFIHGDFREDAVLARLDEVVGGDPVDVVVSDMAPNLSGVAVTDTARVAHLVELAVDFA
                     VTHLKPEGALVTKVFHGSGYSELVELFRANFRVVKAVKPKASRDKSAETFLVGFGLKS
                     PSR"
     gene            1049..1516
                     /locus_tag="VIN75_26435"
     CDS             1049..1516
                     /locus_tag="VIN75_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY8977780.1"
                     /translation="MAMIQLTPAERKEKRGDAHHLDPVVMVGADGLTTAVLKEAEVAI
                     RSHGLIKVRVFSDDRAAREAMLLEMADKLDAAPIQHIGKLLVLWRPVAESASAPSDDA
                     GAAPRVVKILKFSKRGNHRPQIKKVTVMGNQRVTAGGQVKRAKKRVTSIKKKV"
     gene            complement(1500..1985)
                     /locus_tag="VIN75_26440"
     CDS             complement(1500..1985)
                     /locus_tag="VIN75_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY8977781.1"
                     /translation="MATADSIQERLRRLLPGLWCGVLLAIALIAAPAASGTLERASFG
                     AVARAIFAREAPASLVLGMLILMLERRAAFDRHQATGVSQFSAEMMLALGALFCTVAG
                     YYGLQPMMEQARAGGDSALTFMQWHAVSLAFFGVKGLLALALAWRATRPAPRADLRPS
                     S"
     gene            complement(2130..2606)
                     /gene="greA"
                     /locus_tag="VIN75_26445"
     CDS             complement(2130..2606)
                     /gene="greA"
                     /locus_tag="VIN75_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8977782.1"
                     /translation="MATIPLTRRGAEKLKEELHRLKTVERHAVIQAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIEGKLAAAQIIDPSEVDAGGRVVFGSTVVLEDEDSGQE
                     VTYQIVGEDEADLKAGMISIGSPIARAMIGKEAGDTASVQAPGGVKNYEIVAVKYL"
     gene            complement(2706..>5465)
                     /gene="carB"
                     /locus_tag="VIN75_26450"
     CDS             complement(2706..>5465)
                     /gene="carB"
                     /locus_tag="VIN75_26450"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8977783.1"
                     /translation="VVIRPSFTLGGSGGGIAYNPEEFEEICKRGIDLSPTNELLIEES
                     LKGWKEYEMEVVRDRADNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQLMRNA
                     SIAILREIGVDTGGSNVQFSINPVDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAK
                     LAIGYTLDELRNDITGGATPASFEPSIDYVVTKIPRFAFEKFPAADSHLTTQMKSVGE
                     VMAMGRTFQESFQKALRGLETGIDGLTERTACKESDREEILEQITNAGPERILFVGDA
                     FRIGMSLDEVFDATRIDPWFLAQIEDLVKIEQALRGRTVASLSAAELRFVKQKGFSDK
                     RLARLMDGNQHEVRKARHALGVRPVYKRVDTCAAEFSTQTAYMYSCYETEDGENEADP
                     TDKKKIMVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSD
                     RLYFEPVTLEDVLEIVAIEKPAGVIVQYGGQTPLKLALDLEANGVPIIGTTPDSIDIA
                     EDRERFQQLLHTLGLKQPPNRTARTEEAALQLAQEIGYPLVVRPSYVLGGRAMEIVHG
                     DRDLERYMREAVKVSEKSPVLLDRFLDDAIEVDVDCISDGAEVMIGGIMEHIEQAGVH
                     SGDSACSLPPYSLSQGMQAELRRQTALMAKALKVVGLMNVQFAIQGEGDEAVVYVLEV
                     NPRASRTVPFVSKATGQALAKIAARCMAGTKLADQVGLHDKPPVEVIPPYFSVKEAVF
                     PFNKFPGVDPVLGPEMRSTGEVMGVGRTFAEAMLKSQLGAGSRLPTKGNVVLTVKNAD
                     KARTVKVASDLVNLGFTLSATKGTAAAIAEAGIPVKLCNKVKDGRPHIADMIKAGEVQ
                     LVFTTVDETRTAIADSRYIRTAAIANRVTYYTTMAGCEAATEALKHEDDITVISLQEL
                     HAELP"
BASE COUNT          862 a         1843 c         1823 g          937 t
ORIGIN      
        1 gtgatgtcga tgccgccgct accctcggtg atctccacgc tctggatgtg cttgttgcgc
       61 acttcctcga ggaaatcgga atatgcgacc cgattgccct gggtctgcgc cgtgttgaac
      121 gacttgaaga ccgtgaaaag cacgagggcg atcaccagcc agaccgcgat cttcgaaaac
      181 cactgattgt tcaccgcggc tccttcttca aaatgccact tcccgcgaca ggtggggatg
      241 attctaggcc agtcaatacc ccgcaaaccc gatagccgcg gtcgagaagg acgccaatcg
      301 cacgttttgg acgcgaaatg ctccctccgc gccacgccca ccgggcaatt ggacggccca
      361 ggtcagcgcg acgggctctt cagaccgaag ccgaccagga aagtttctgc cgacttgtcg
      421 cgcgaggcct tgggcttgac cgccttgacc acccggaaat tcgcacggaa cagttccacc
      481 agctcactgt agccgcttcc gtgaaagacc ttggtcacca gggcgccttc cggcttgagg
      541 tgcgtcacgg cgaaatcgac ggccagctcg accaggtggg cgacgcgggc ggtgtcggtg
      601 accgcgacgc cgctgaggtt gggcgccatg tccgagacga cgacgtcgac cggatcgccg
      661 cccaccacct cgtccaggcg cgccagcacc gcgtcctcgc ggaagtcgcc gtggatgaag
      721 gtgacgccct cgatgggctc catctccagg atgtcgagcg ccacgatgcg gccgttcagc
      781 gcgcccaccg ccgccccgcc gccggcgaac ttgcggcgga tgtactggct ccaggcgccg
      841 ggcgtcgagc ccaggtcgac caccagctgg cccgggcgga tcagcttgaa ctgctcgtcg
      901 atctcggaca gcttgtaggc cgcgcgggcg cgatagccct ccttctgcgc gagcttcaca
      961 taggtgtcat tggcgtgatc ctgcaaccac tgcttgttga ccttcttgct tttcgtgttg
     1021 attttcatga caaagggata atacgggcat ggccatgatt caactgaccc ctgccgaacg
     1081 caaagaaaag cgcggcgacg cccaccacct cgaccccgtg gtcatggtcg gtgccgacgg
     1141 actgaccacc gccgtcctca aggaggccga ggtcgcgatc cgctcgcacg gcctgatcaa
     1201 ggtgcgcgtg ttctcggacg accgcgccgc ccgcgaggcg atgctgctgg agatggccga
     1261 caagctcgac gccgccccca tccagcatat cggcaagctg ctggtgctgt ggcgccccgt
     1321 ggccgagtcc gcgtccgcgc cgtccgacga cgcgggcgcc gccccgcgcg tcgtcaagat
     1381 cctgaagttc tccaagcgcg gcaatcaccg tccgcagatc aagaaggtca ccgtgatggg
     1441 caaccagcgc gtgaccgccg gcggccaggt caagcgcgcc aagaagcgcg tgaccagcat
     1501 caagaagaag gtctgagatc cgcgcgtggc gcgggacgcg tcgcgcgcca cgccagcgcc
     1561 agggccagca ggcccttgac gccgaagaac gcgaggctga ccgcgtgcca ctgcatgaag
     1621 gtcagcgccg agtcgccgcc ggcacgcgcc tgctccatca tcggctgcag gccgtagtag
     1681 ccggcgacag tgcagaacag cgcaccgagc gccagcatca tctccgccga gaattgcgac
     1741 acgcccgtcg cctggtggcg gtcgaacgcc gcgcgacgct ccagcatcag gatcagcatg
     1801 ccgagcacga ggctggcggg cgcctcgcgg gcgaagatcg cccgcgccac ggcgccgaag
     1861 ctggcccgct ccagcgtgcc cgacgcggcc ggcgcggcga tcagcgcgat cgccagcagc
     1921 acgccgcacc acaggccggg cagcaggcgg cgcaggcgct cctggattga atcagcggtt
     1981 gccatttgaa tgaccggtgg tcggacacgg ttccgacgct gtcgctggca ctccgaaggc
     2041 gaaaaggcga gggagcggcg gaaccggctc tgccgggccg ctcgcggagc gccccctcgg
     2101 ggggcagcga ccagcgggag cgtgggggct caaagatact tcacggcgac gatctcgtag
     2161 ttcttgacgc cgccgggcgc ctgcacggag gccgtgtcgc cggcttcctt gccgatcatc
     2221 gcgcgggcga tcggcgagcc gatcgagatc atgccggcct tgaggtcggc ctcgtcctcg
     2281 ccgacgatct ggtacgtgac ctcctggccc gaatcctcgt cctcgagcac cacggtcgag
     2341 ccgaacacga cgcggccgcc cgcgtcgact tccgacggat cgatgatctg ggccgcggcg
     2401 agcttgccct cgatctcgag gatgcggcct tcgatgaagc cctgcttgtc cttggccgcc
     2461 tcgtactcgg cgttctcgga aaggtcgccc tgggcgcgcg cttccgcgat ggcctggatg
     2521 acggcgtggc gctcgaccgt tttcaggcga tgcagctctt ccttcagttt ctcggcgccg
     2581 cggcgggtca ggggaatcgt ggccatatca gggtctcagc gggggaaaaa gcaaaccgcc
     2641 gccccggctg cgcttgcgtg gcgcagacgg gcggcggccc ttgtggaatg gaagtctaaa
     2701 tcagatcagg gcagttccgc gtgcagttcc tgcagggaaa tcaccgtgat gtcgtcctcg
     2761 tgcttcagcg cctcggtggc ggcctcgcag ccggccatcg tcgtgtagta cgtgacgcgg
     2821 ttggcgatcg cggcggtgcg gatgtagcgc gaatcggcaa tcgccgtgcg cgtctcgtcc
     2881 accgtcgtga acaccagctg gacctcgccg gccttgatca tgtcggcgat gtgcggccgg
     2941 ccgtccttga ccttgttgca cagcttgacc gggatgccgg cctcggcgat cgccgccgcc
     3001 gtgcccttgg tggccgacag cgtgaagccc aggttgacga gatccgaagc gaccttgacc
     3061 gtgcgcgcct tgtcggcgtt cttgacggtc agcacgacgt tgcccttggt cggcagccgc
     3121 gagcccgcgc ccagctggct cttgagcatg gcttccgcga acgtgcggcc cacgcccatc
     3181 acctcgccgg tggagcgcat ctcggggccg agcaccggat cgacgcccgg gaacttgttg
     3241 aacgggaaga cggcttcctt gaccgagaag tacggcggga tcacctcgac cggcggcttg
     3301 tcgtgcaggc cgacctggtc cgccagcttc gtgccggcca tgcagcgcgc ggcgatcttg
     3361 gccaacgcct ggcccgtggc cttggacacg aacggcaccg tgcgcgaggc gcgcgggttg
     3421 acctcgagca cgtagacgac ggcctcgtcg ccctcgccct ggatcgcgaa ctgcacgttc
     3481 atcaggccga cgaccttcag cgccttggcc atcagcgccg tctggcggcg cagctcggcc
     3541 tgcatgccct gcgacagcga gtacggcggc agcgagcacg cggagtcgcc ggagtgcacg
     3601 ccggcctgct cgatgtgctc catgatgccg ccgatcatga cctcggcgcc gtcgctgatg
     3661 cagtcgacgt cgacttcgat cgcgtcgtcc aggaagcgat cgagcagcac cggcgacttc
     3721 tcgctgacct tgacggcctc gcgcatgtag cgctcgagat ccctgtcgcc gtgcacgatc
     3781 tccatcgcgc ggccaccgag cacgtagctg ggccgcacga ccagcggata gccgatctcc
     3841 tgggccagct gcagcgcggc ttcctccgtg cgcgcggtgc ggttgggcgg ttgcttcagg
     3901 cccagcgtgt gcagcagctg ctggaagcgc tcgcggtctt ccgcgatgtc gatggagtcg
     3961 ggcgtggtgc cgatgatggg cacgccgttg gcctcgaggt cgagcgccag cttcagcggc
     4021 gtctggccgc cgtactgcac gatcacgccg gcgggcttct cgatggccac gatctcgagc
     4081 acgtcctcga gcgtcaccgg ctcgaagtac aggcggtcgg aggtgtcgta gtcggtcgag
     4141 accgtctcgg ggttgcagtt gaccatgatg gtctcgtagc cgtcctcgcg catcgcgagg
     4201 gccgcgtgga cgcagcagta gtcgaactcg atgccctggc cgatgcggtt cggaccgccg
     4261 cccagcacca tgatcttctt cttgtccgtc gggtcggcct cgttctcgcc gtcctcggtc
     4321 tcgtagcacg agtacatgta ggcggtctgc gtggagaact cggcggcgca ggtgtcgacc
     4381 cgcttgtaga cggggcgcac gcccagcgca tggcgcgcct tgcgcacctc gtgctggttg
     4441 ccgtccatca gcctggccag gcgcttgtcg gagaagccct tctgcttgac gaagcgcagc
     4501 tcggcggccg acagcgacgc gacggtgcgg ccgcgcagcg cctgctcgat cttcaccagg
     4561 tcctcgatct gcgcgaggaa ccacgggtcg atgcgcgtgg cgtcgaagac ctcgtccagc
     4621 gacatgccga tgcggaacgc gtcgcccacg aacaggatgc gctcggggcc ggcgttggtg
     4681 atctgctcga ggatctcctc gcgatcgctt tccttgcagg cggtgcgctc ggtcaggccg
     4741 tcgatgccgg tctccaggcc gcgcagcgcc ttctggaagc tctcctggaa cgtgcggccc
     4801 atcgccatga cttcgccgac cgacttcatc tgcgtggtca ggtgcgaatc ggcggccggg
     4861 aacttctcga acgcgaagcg cgggatcttg gtgacgacgt agtcgatcga cggctcgaac
     4921 gacgccggcg tcgcgccgcc ggtgatgtcg ttgcgcagtt catcgagcgt gtagccgatg
     4981 gccagcttgg ccgcgatctt ggcgatcggg aaacccgtgg ccttggacgc gagcgccgac
     5041 gagcgcgaca cgcgcgggtt catctcgatg acgaccatgc ggccgtcgac cgggttgatc
     5101 gagaactgga cgttcgagcc gccggtgtcc acgccgatct cgcgcaggat cgcgatcgac
     5161 gcgttgcgca tcagctggta ttccttgtcg gtcagcgtct gcgccggcgc gaccgtgatc
     5221 gagtcgcccg tgtggatgcc catcgggtcg aggttctcga tcgagcagat gatgatgcag
     5281 ttgtccgcgc ggtcgcggac gacttccatc tcgtattcct tccagccctt gagcgactcc
     5341 tcgatcagca gctcgttggt gggagacagg tcgatgccgc gcttgcagat ctcctcgaac
     5401 tcctccgggt tgtaggcgat gccgcccccc gagccgccga gcgtgaacga cgggcggatc
     5461 acgac
//
LOCUS       DATCRO010000968        19250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_955443, whole genome shotgun sequence.
ACCESSION   DATCRO010000968 DATCRO010000000
VERSION     DATCRO010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955443"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2568)
                     /locus_tag="VIN75_26455"
     CDS             complement(199..2568)
                     /locus_tag="VIN75_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY8977784.1"
                     /translation="MSDKRTNLRHPSTHPVDRGDPPDPARRALSMGLGLFPMLPLLPA
                     CSAGFEGDGQSTSGPGQAADGSSASPTGAAPAPAPASAPAPGTAPAPAPGPAPAPAPV
                     PASVSGVFRHPGLLVTEDDFTRIRALIRTGQQPWTNWWNTLCTDHFDTLDTKPYPLPA
                     VYRADGSKNNLYVDIWRAWSMTLRWKLGDPQDTRYADKAVEFLDAWAGTLKEVGTVPP
                     GSTAHDDHTFIILAGIQGHQLAQIGEVLRTYPGWVPESLKRFQDMLSTVFAPISSWFL
                     SDGRIGSHANWDMASMAGAMAIGVFCDNAELYRLACDCYAANNRGKLRNFGNGSALHG
                     VYYMHPGHFGQWEESGRDQGHSTLGMSLGGDVMEMAWNQGDDLYGLYNNRFLAAAEYV
                     ARSNLLDENGKTYPMPYVPEQNPTSPHTDRWTQVNQSFQHGRNAWEPIYNHYVNRMGI
                     AAPNVARMVKWVEPKYGGSSDDVWYPTLIHRRVDYAGPMKPASGLTGHLNDGRVLLSW
                     WGSVGAASYVVKRGATANGPFAQLGTVAADQLLTFTDTPPGGVWFYQVVAQGASGPAS
                     AASNVVRVAASGESRLTMPLNGMNGTGTSGTFLAADGTSSTVQGKLLDNATWGDGRNG
                     DKAVAFDGKASGLQLPQGLFGGLDDFSVSLWAYANGLHWDTCMLFAGTDAFSSLFIAP
                     QAGPGVLRFGIYGATFNDAHVIDAPSALPTRRWVHVAVTLQGTTGRLYLDGLEVAKSD
                     DILLTPRQVGDQVTFLGRNWAHPPFNGRIQDFRIHAGALSAAEVAALAR"
     gene            2786..4015
                     /locus_tag="VIN75_26460"
     CDS             2786..4015
                     /locus_tag="VIN75_26460"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8977785.1"
                     /translation="MLSTALLATERHHAMQRRANRNAVIRHLCARPGSSAPELAIALG
                     LPMSVVAPLLRQFMLEGLLLESRERGAAAGRVLPRYRLDATHLALLGADVGADRVRVV
                     ATSLDGRVLAGTMLEYGRASSAMRRIDSLATAALRVCEKLGAAAPHVIGMGLGLPGSA
                     EAPAGESHGDSLPRSRDVPFASLLARELERSHLEGMPLFLGSSADVAALGEFDFAPAP
                     DGPSLLYLSLDEDLDAGVIVDGEVLPARRRRTNGIGHTVLQVDGPRCSCGRSGCAQAL
                     IGSRSLLGTAEPDAVGVLGRRLAAGSPETVSAVARAGACLGTLLHNLAILYRPSRIVV
                     GGSLTELGEALLQPARLSLGECAASGVALATSRFGGDAVAVGAAALARRRLCGRPAPV
                     APAAPSRCDATRTAAFF"
     gene            4171..5802
                     /locus_tag="VIN75_26465"
     CDS             4171..5802
                     /locus_tag="VIN75_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY8977786.1"
                     /translation="MTLTRRSFLHSAAAASALATPVSQAASPAFAPQWDSLAEGYRTP
                     DWFRDAKFGIWAHWTAQCVPEMGDWYARNMYLQGNWQYDHHVKTYGHPTQVGFMEMQN
                     RWKAEHWDPDVLLDLYVKAGARYFVALANHHDNFDAYDSKYHGWNSVRVGPRRDIVGT
                     WAKAARARGLRFGVSNHSGHAWHWLQAAYGYDPEGPLAGQRYDAWKLTQADGRGKWWD
                     GLDPQDLYTGRNMVMPDGIETIRTANEWHEQNDRQWDEKAPLQNPAFTRRWFLRCRDL
                     IDSYRPDLVYFDNSGLPLGQAGLDITAHYYNASMAWHGGKLEAVVNAKVLPPERRAAL
                     VEDVERGFREGIEPLPWQTDTCLGDWHYNRSLFDNHRYKDATTVIHRLCDIVSKNGNL
                     LLSVPLRGDGTIDSDERKVLEDIAAWMGRNGEAIHGTRPWRSFGEGPTRVGGGMFSEG
                     SSQPFSAQDIRFTAKDGALYAIALGWPQGGRLRIAALAQDSALAPGAIERVEALGAGD
                     SLPFTRSNRGLEVKLPEGLAGMPAVALKIRGPGLA"
     gene            5915..7456
                     /locus_tag="VIN75_26470"
     CDS             5915..7456
                     /locus_tag="VIN75_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8977787.1"
                     /translation="MNMHFHRRLLAACALISFLNVAAAVEPAQAELVVDAQAAGPRID
                     PDVYGQFAEQLGAGIYGGLWVGPESPIPNTRGWRNDVVQALRHLEVPVVRWPGGCFAD
                     DYDWRDGIGDPARRPVRLNRWWGGVPESNRVGTHEFMDLAEQIGAEVVIAGNMGSMSP
                     RDMSQWMEYMTATGPSALTDERRRNGRDKPWKVKYIGIGNESWGCGGNMRPEYQADLH
                     NRYATFLNGGALRVASGDGTSNDHLIDVLMERSIDQMDALTLHYYTLPGDWKKKGAAT
                     GFDTAEWAHTLKVARGIEARITAIEAIMDKRDPKRRVGLFIDEWGTWYDTEPGANPGF
                     LYQQNTLRDALVAALTLDVFHRHTDRVRMTSIAQMVNVLQAMVLADGSRMVLTPTYHV
                     YDMYKPFKGATPLDSRLDTSKWHRGDDELPAVDASVARTPDGATVLAIVNLDPERPAH
                     VRTNLQGDFRGSMLTAAKMDAHNTFDQPHAIEPAPFAAHGGAAGLTLDLPPKTVLVVS
                     APARH"
     gene            7475..8605
                     /locus_tag="VIN75_26475"
     CDS             7475..8605
                     /locus_tag="VIN75_26475"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY8977788.1"
                     /translation="MARHSLTKFPAPALLLAAAALLAGCAATPEAARPLATAAAPSSM
                     AELTTQMTPGINLGNTMEALPNETAWGNPVPSQALMDGYRAAGFRSVRIPLAWRPYED
                     SRHQIDHKWMAHVREVVDQARRAGLYVMINTHWDGGWMNHPTYDHQAAIQAQLAAYWS
                     QIATEFKDYDEHLLFAGSNEVGVENSVAPPTPEQAAVQNAFNQTFVDTVRAAGGRNAT
                     RYLVVQSFSTNIGHAIQFSTIPRDPVPGRLLMEVHYYDPFNFTINGKSTVWQWGKRAT
                     DRAASDPWADEPWVDKQFDSMKTHYVDHGVGVIVGEYGAYAKPEFPGSAPYIRDWARY
                     VTGAIRRNGLVPMWWDTGALVDRRTGAQKDPELIQAIVGASR"
     gene            complement(8635..9855)
                     /locus_tag="VIN75_26480"
     CDS             complement(8635..9855)
                     /locus_tag="VIN75_26480"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8977789.1"
                     /translation="MTADSAGHQQLLKDINRMSLVRQLFAHPGMSRADLAAAVGLTKS
                     TVSTIVRELIEEGWMCEREVVATGDLGRRPTPMFIDQERLLLIGAEVDIESARVVATS
                     LTGDILASATTNFGSSKSAKACIGGLANAMLNVRAQLNSSRQRVVGIGVGLPGGVDED
                     TGFLHFAPNLGWRDVPVGAMLREKLAGTALSGVPLFVQNEADVAALGEMEFNPSIASD
                     PLLYLSMNQGVGAGVIFSDRLFTGRRGFAGEVGHMVLHINGPMCSCGRRGCAEALIGP
                     RSILGAPEDHPHPLREIRRLVQERHPETLRAVKKAGGYLGVLLQNLASAYDPGSIVIG
                     GQMAELGEVFLQPALRTLTEYAAAANLPAPNVRSSRFGRDAVAVGAAALVRYRLTRPL
                     MATLDNEASIEMAA"
     gene            complement(10308..11672)
                     /locus_tag="VIN75_26485"
     CDS             complement(10308..11672)
                     /locus_tag="VIN75_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY8977790.1"
                     /translation="MSDGGRAGKVSRRSLLCAAAVAPLLPRRAAVAAAPDGVTDEQRS
                     ALKGANAPASLAPDLLLPPQDLDWWRDAKFGVFVHWGLYAIAARGEWHMFNDKVPPDE
                     YAALARRFSPRHYDPDAWAALASQAGARYMVMTTRHHDGFSLFASQASYGHFDAPHSA
                     ARRDLIAPWVRAVRRHGLRVGFYYSPLDWRFPAYFHPREMPDNARLFKQQTYGQVEEL
                     MRNYGPIDILWYDGGWLAHQGTDADAAWFWEPDKLNRMVRRYQPRIVINPRSGWQGDY
                     DEEEGPAPIKGPIRPRPWEKTFSLNSAWGYVPEVHVMSADEVVTLLVDAVVRNGNVLA
                     NVGPDADGVIPPMQARTLREVGRWLERYGHAVYGTRPGPLQPVDGLWGMTQQGDRAYL
                     FVHAWPTAELVLPPMPAGVTSLRNLSGEGVAWRSGPDGLAVSVEPQYRGQPVTVLEIV
                     MAAR"
     gene            complement(11810..15001)
                     /locus_tag="VIN75_26490"
     CDS             complement(11810..15001)
                     /locus_tag="VIN75_26490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8977791.1"
                     /translation="MRRHFSKTALSVAAAQVLLLCSGSALAQAAAPAPGDAPSAPAAS
                     ASSPAAAASANAAKAAPAEADRDSQQVVVTGQRAALQSAQAIKKNAEQIVDAVVAEEA
                     GKLPDKSITEVLQRVVGVTMDRNKSPGDPEHFSVEGSGVQVRGLSWGSSTLNGRESFS
                     AGYPGRALSWGDVPPELMAAVLVYKNPSSELIEGGISGQVDLRTAMPFDFKETKFSIS
                     ANDNYTVMGSKKSPGVSGIYSRRWDSSVGQFGALVDLSFNQTNTHNDTIELDAYFPRN
                     DLVAGKTVWIPQSASWRTNTSQDNRAGLYAALQWKNDDKESSLTIFNSFYKDTSAEYA
                     LHANGINPYLIQVDPQSAVYSGNVLTSGTYTVPMGQLGSNEFVAGGLSYDTDVGRSVS
                     HSQTRDIAWNFKWDVSDHLALQNDLQWVHASDDSFNNYAAIGTFVPELTLSTAHGGPP
                     SLTFNPSTVAFLANPANYYMDNEMPMQSKATGNMVAWKGDARFKFDSDVLRDFRLGMR
                     VTNRSAKYTNADGTGWFSFSDPWNVRQTQMPGTLPNNSDQPSWGTFANVGYLKPGSVY
                     ANLVPTQVQSFPNFFNGKVAQLPALVFPSMSLPGGYPNSYQPLTDILVQECKDGNAQF
                     GSSNTCQPPTWTPLSYDGNPAQVSRTDEQSQALYGTLRFAEDGWRFPVEGNVGLRVVH
                     TRTISHAYTVFTPGTNPPPEVPQFGQINAAHTLVTSYTDFLPSLNVKTDLTSKLQGRF
                     AASKGIYRPDFGQLQGYETLNQNVSTNNNGAITSITYTGNSNGNPRLKPIRSNNLDLS
                     LEWNPTWGQSLTAAVFYKQLRDIILPGAHIERYADLAGNLQDFTVTGPDNLAKGSVAG
                     FEVGGMTYLDKFAALERLLPDWAKGFGISANFTYIDSHQQLYHPFDLKYCPAASAFNN
                     SNLNVYGCDTNGLPFKSLPIQYLSRDAFNLAFLYDHGPLSARLAYNWRSRYLQGVNNN
                     STSGTNATSADPARPGAQDVGWGLPVWLEGLGQWDAGVDYKMLGDRWLVSLSVTNLTN
                     TMGRQTQQQAPGYMGRAWYEYGRSFRLSTRYEF"
     gene            complement(15229..16569)
                     /locus_tag="VIN75_26495"
     CDS             complement(15229..16569)
                     /locus_tag="VIN75_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977792.1"
                     /translation="MRQATMLSKASTVVVAFGALLSACGGDGGSSGGTAQSIDFPYPG
                     TRYLATAAAPLSASATSGLTVTLTSNTPSVCTISDGKLVPVAVGECQVTATQDGDGSF
                     AAAAPTQQLFKVLPHPQAITFPSPGFLALGAQPTTLTATSDSGLAVTYTSLTPTSCTV
                     SGNTAMAAGSGQCTIEATQPGDANYAAAAPVDVTVNAGDAPPPTLTVASGFQGTSATT
                     DGGSIGTGAGANIDNWWCSNDQWCWSALSPDNSVLTYTFVLQPTDPAAVQTTPIYAYD
                     NGITINVPGLTNGISQTGNTAEGLQVANQTTLSFTLGENPEWFSLLSPGSPHGADVLV
                     TLTLGHFNQFQDKPCNVKVQASVTPTAAAQAAYQVSLSNFNVVSTNCGLSGLVAATEL
                     TSYPIVQVQFSPASTNASVNGTAPVNQGAQHYPYTPYSPAYATVISVGTPILIQ"
     gene            17391..17891
                     /locus_tag="VIN75_26500"
     CDS             17391..17891
                     /locus_tag="VIN75_26500"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8977793.1"
                     /translation="MSHGDSESPARRLSPAEFTEVPMLELSQGAYSKNAPVRVDARAD
                     LPLRPSPETGLRFGRCEVRPSRRELRVDGQLRQIQPRPFDLLVYLIDHRDRVLSMDEL
                     LDVVWGQRIVQPSSLTVAINRIRSVLGDERGEIIRTHHRVGYRFVAELKADELHFAPR
                     QQAAIA"
     gene            17892..18824
                     /locus_tag="VIN75_26505"
     CDS             17892..18824
                     /locus_tag="VIN75_26505"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8977794.1"
                     /translation="MATRGILVDHRLILLLAALATACTAPQPGGTVSPAQARANFARP
                     VVLAADDVRAYPVPPRGFADPKPGVAPGRIEDLSYLSAVTGTRRQARIYLPAGYQASR
                     RYPVLYLLHGIAGNQDEWRGYVRAPTILDNLQAAGRMVPMIVVMPNGRALADDAPPPA
                     DKVFTPANAAGFDRFERDLLDFLVPAVDSTYPTLADSGHRAIAGLSMGGGQALNFGLA
                     HLDRFAWVGAFSPAPNTRGIADLIPDPVDARRGLRLLYLSCGDQDGLINVSQDVHRGL
                     AQRAVPHLWNVDDHGHDRDSWAENLYNFAQLIFR"
     gene            complement(18845..>19250)
                     /locus_tag="VIN75_26510"
     CDS             complement(18845..>19250)
                     /locus_tag="VIN75_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY8977795.1"
                     /translation="FTKIGGWRISPPQGDRPDPGTAPSYGDMNTWGWGEPPMKQGPEG
                     QPWRVYRASLNVRADRNDGRGRLVFREIAGRAEVWCEGVKLGEKTDPAPGPLTLQLPQ
                     GPAWRTLNVLVQSQPGQDSGIVGRVVLEPGGD"
BASE COUNT         3022 a         6521 c         6585 g         3122 t
ORIGIN      
        1 ggcgggggag gacgcgccgc cgctggcgcc ggtcgtggtc gatccattgg aggatcccga
       61 gccgccgccg ccacaggcgg tcagcgccgc cgcaagggca aaggcaaggc gaaattgaaa
      121 ggcgttcatt tgggaggagt cttgtttgaa gagggtgatg cgacggcgac gcactggtcg
      181 atgtccgatg ggaagccgct agcgcgccag cgcagcgacc tccgcagcgc tcagggcgcc
      241 cgcatggatg cggaagtcct ggatgcggcc gttgaacggc ggatgcgccc agttgcggcc
      301 gaggaaggtg acctggtcgc cgacctggcg cggcgtcagc aggatgtcgt cggacttggc
      361 cacctcgagg ccatccaggt agaggcgtcc ggtcgtgccc tgcagcgtga ccgccacgtg
      421 gacccagcgc ctcgtgggca gggcgctggg tgcgtcgatg acgtgggcgt cgttgaacgt
      481 ggccccgtag atcccgaaac gcaggacccc cgggccggcc tggggtgcga tgaacaggct
      541 ggagaaggca tcggtcccgg caaagagcat gcaggtgtcc cagtgcaggc cgttcgcgta
      601 ggcccacaac gacacgctga agtcgtccag accgccgaac aggccttgcg ggagctgcag
      661 gcccgaggcc ttgccgtcga acgcgacggc cttgtcgccg ttgcgcccgt cgccccaggt
      721 ggcgttgtcg agcagcttgc cttgcacggt gctggaggta ccgtcagcgg ccaggaaggt
      781 gccggaggtg cccgtgccat tcatgccgtt cagcggcatc gtcaggcgcg attcgccgga
      841 cgccgcgacc cggaccacgt tggaggcggc actggcgggc ccgctggcgc cctgggcgac
      901 gacctggtag aaccagacgc cgccgggcgg cgtatcggtg aaggtcagga gctggtccgc
      961 cgcgaccgtg cccagctggg cgaaggggcc gttggccgtc gcgccgcgct tgaccacata
     1021 ggacgccgcc cccacgctac cccaccacga cagcaggacg cggccgtcgt tcaggtgccc
     1081 cgtgaggccg gatgccggct tcatcggccc ggcgtagtcg acgcgccggt ggatcagcgt
     1141 cggataccac acgtcgtcgc tgctgccgcc gtacttcggc tcgacccact tgaccatgcg
     1201 tgcgacgttc ggcgccgcga tgcccatgcg gttgacgtag tggttgtaga tcggttccca
     1261 ggcgttgcgg ccgtgctgga acgactggtt cacctgcgtc caccggtccg tgtgcggcga
     1321 cgtcgggttc tgctccggca catagggcat cggataggtc ttgccgttct catccagcag
     1381 gttggaccgg gccacgtact cggcggccgc gaggaagcgg ttgttgtaca gcccgtagag
     1441 gtcgtcgccc tggttccagg ccatctccat cacgtcgccg ccgagggaca tgcccagcgt
     1501 cgagtggccc tggtcgcggc cgctctcctc ccactgcccg aagtggccgg gatgcatgta
     1561 gtagacgccg tgcagggccg agccgttgcc gaagttccgg agcttgccgc ggttgttggc
     1621 ggcgtagcaa tcgcaggcca gcctgtacag ctcggcgttg tcgcagaaga cgccgatggc
     1681 catcgcgcct gccatcgagg ccatgtccca gttggcgtgg gagccgatgc ggccgtcgct
     1741 gaggaaccac gacgagatcg gcgcgaagac cgtgctgagc atgtcctgga agcgcttcag
     1801 gctctccggc acccagccgg ggtaggtgcg caggacctcg ccgatctgcg cgagctggtg
     1861 cccctggatg ccggcaagga tgatgaaggt gtggtcgtcg tgcgccgtcg agcccggcgg
     1921 gacggtgccc acttccttga gcgtgcccgc ccaggcgtcc aggaactcca ccgccttgtc
     1981 ggcgtagcgg gtgtcctgcg ggtcgcccag cttccagcgc agcgtcatgg accaggcgcg
     2041 ccagatgtcg acgtacaggt tgttcttgct gccgtcggcg cggtagacgg ccggcagcgg
     2101 gtacggcttg gtgtccaggg tgtcgaaatg atcggtgcag agcgtgttcc accagttcgt
     2161 ccacggttgc tggcccgtcc ggatcagcgc gcggatacgg gtgaagtcgt cttcggtgac
     2221 cagcagcccg ggatggcgga agaccccgct caccgacgcc gggaccggag caggtgccgg
     2281 ggccgggccg ggggcagggg caggggcagt gccgggggcc ggcgcagatg ccggcgcggg
     2341 ggcgggcgcc gcgcccgtcg ggctggcaga ggagccgtcg gccgcctggc ccggcccgga
     2401 cgtcgactgg ccgtcgccct cgaagcccgc actgcaggcc ggaagcagcg gcagcatcgg
     2461 gaacaggccc aggcccatgg acagcgctcg acgggctgga tcgggcggat cgccgcggtc
     2521 gacggggtgg gtggatgggt gccgcaggtt ggttcgcttg tcgctcatcg ggcgcaaact
     2581 aacgcacgcc ctcggcacag tcatgaccaa gatctgaccg cgcaacgaca tcacgcagga
     2641 ctcaccggct gttactcgcc cgccccggag aacgtcgagc cgggcgccag cctccattga
     2701 catggtcgag agacggatag gtatttgccc tctttagttc gaccatcgaa cgaactacgc
     2761 tgacaacgca gacgagggac ttcacgtgct atccaccgcg cttctcgcga cagaacgcca
     2821 ccatgccatg cagcggcgcg ccaaccggaa cgcggtgatc cgccatcttt gcgctcgtcc
     2881 gggcagctct gcgccggagc ttgcgatcgc gctcggcctg ccgatgtcgg tcgtcgcccc
     2941 gttgctgcgc cagttcatgc tcgagggcct gctcctcgaa agccgcgagc gcggcgcggc
     3001 agccggacgc gtcctgcccc ggtatcgcct cgacgcgacg cacctcgcgc tcctcggcgc
     3061 ggacgtcggt gcggaccggg tccgcgtcgt cgccacgtcg ctggatggcc gtgttctggc
     3121 gggcaccatg ctggaatacg gacgggcatc cagcgcgatg cgtcgcatcg attcgctggc
     3181 caccgccgcg ctgcgcgtct gcgagaagct gggcgccgca gcgccgcacg tcatcggcat
     3241 ggggctcggc ctgccaggca gtgcggaggc accagcaggc gaatcgcacg gcgactcgtt
     3301 gccgcgctct cgcgacgtcc cgttcgcgtc gctgttggca cgcgaactgg agcgatccca
     3361 tctcgagggg atgccgctgt tcctgggctc ctcggccgat gtcgcggcgc tgggcgaatt
     3421 cgacttcgcc cccgcccccg acggtccatc cttgctgtac ctgagcctgg acgaggacct
     3481 ggacgccggc gtcatcgtcg acggtgaagt gctcccggcc cggcgacggc gcacgaacgg
     3541 gatcggccac accgtcttgc aggtggacgg gccgcgatgc tcctgcggcc gttcgggctg
     3601 tgcgcaggcg ctgatcggct cgcgcagcct gctgggcacg gcagagcccg atgctgtcgg
     3661 ggtgcttggg cgccggctcg ccgcgggttc gccagagacc gtgtcggcag tggcccgggc
     3721 gggcgcctgc ctggggacgc tgctgcacaa cctggcgatc ctctaccggc cgtccaggat
     3781 cgtggtcggc ggttcgctga ccgaactggg cgaggcgctg ctgcagcccg cgcgcctgtc
     3841 cttgggcgaa tgcgctgcgt cgggcgtcgc cctcgcgacg tcacgcttcg gtggcgacgc
     3901 ggtcgccgtc ggcgctgccg cgctggcgcg ccgccgcctc tgcgggcgtc ctgcccctgt
     3961 ggcgcccgcg gcgccgtccc gctgcgacgc gacgcgcacg gcagcgtttt tttgacaatt
     4021 tgatttatcg atatatcacc tcaggaacgc accggaatcc gcacctctgg acgacgctgc
     4081 aggcaccgcc ggaacgcgcg ccagtcgccc ccggagaatc tttcttccga cttcaaaaga
     4141 ccaatgatgc aaagagacaa atcccgccga atgacgttga cgcgtcgctc cttcctgcac
     4201 agcgcggccg ccgcgagcgc cctggcaacg cccgtctcgc aggccgcctc cccggcgttc
     4261 gcaccccagt gggactccct ggccgagggc taccgcacgc ccgactggtt ccgcgatgcc
     4321 aagttcggga tctgggctca ctggacggcg cagtgcgttc cggagatggg ggactggtat
     4381 gcccgcaaca tgtatctcca gggcaactgg cagtacgacc atcacgtcaa gacctacggg
     4441 cacccgaccc aggtgggctt catggagatg cagaaccgat ggaaggccga gcactgggac
     4501 cccgatgtcc tgctcgatct ctacgtgaag gccggcgcac gctacttcgt cgcgctcgcg
     4561 aaccatcatg acaacttcga cgcctacgat tcgaagtacc acggctggaa cagcgtccgc
     4621 gtcggtcctc gacgcgacat cgtcggcacc tgggccaagg cggcgcgcgc gcgcggcctg
     4681 cgtttcggcg tcagcaacca ttcgggtcat gcatggcact ggctgcaggc cgcctacggg
     4741 tacgacccgg agggcccgtt ggcaggccag cgctacgacg cctggaagct gacccaggcg
     4801 gatggacgcg gcaaatggtg ggacgggctc gacccgcagg atctgtacac cggccgcaac
     4861 atggtgatgc ccgacggcat cgagaccatt cgcaccgcca atgaatggca cgagcagaac
     4921 gatcgccaat gggacgagaa ggccccgctg cagaacccgg ccttcaccag gcgctggttc
     4981 ctgcgctgcc gcgacctcat cgacagctac cgaccggacc tcgtctattt cgacaattcg
     5041 ggcctgcccc tcggccaggc gggcctggac atcacggccc actactacaa cgccagcatg
     5101 gcgtggcatg gcggcaagct ggaagcggtc gtcaacgcga aggtgctgcc acccgaacgg
     5161 cgagccgcgc tggtcgagga cgtggaacgc ggcttccgcg agggcatcga gcccctgccc
     5221 tggcagacgg acacgtgcct gggcgactgg cactacaacc gctcgctctt cgacaaccac
     5281 cgctacaagg acgcgaccac ggtcatccat cgcctgtgcg acatcgtgtc caagaacggc
     5341 aacctgctgc tgtccgtgcc gctgcgcggc gacgggacca tcgactcgga cgagcgcaag
     5401 gtcctggagg acatcgccgc ctggatgggc cgcaacggcg aggccatcca cgggacgcgg
     5461 ccctggcgca gcttcggcga agggccgacc cgcgtgggcg gcggcatgtt cagcgagggc
     5521 agctcgcagc cgttctcggc gcaggacatc cgcttcacgg ccaaggacgg cgcgctgtac
     5581 gcgatcgccc tgggatggcc gcagggcggc cggctgcgca tcgcggcact ggcgcaggac
     5641 tctgcgctgg cgccgggcgc gatcgagcgc gtggaggcgc tcggcgccgg cgacagcctg
     5701 ccgttcaccc gcagcaatcg cgggctggag gtcaagctgc ccgaaggcct tgcaggcatg
     5761 cccgcggttg cgctcaagat ccgcggtccg ggcttggcgt gagacgctgg atccatcacg
     5821 cccaccccga cacctccgcc cggtcagacg accgcggcgt ccactgttcc ccgccgcaag
     5881 gccgtgcgcc atgcggccag ctcgcccatc gaaaatgaac atgcacttcc atcgcagact
     5941 gctcgccgcc tgtgcgctca tctccttcct gaacgtcgcg gccgcggtcg aacccgcgca
     6001 ggccgagctc gtcgtcgacg cgcaagcggc cggcccgcgc atcgaccctg acgtctatgg
     6061 ccagttcgcg gagcagctgg gggccggcat ctacggcggc ctctgggtcg gcccggaatc
     6121 gccgattccc aacacgcgcg gctggcgcaa cgacgtggtg caggcgctgc gccacctcga
     6181 ggtgccggtg gtgcgctggc cgggcggctg cttcgccgac gactacgact ggcgcgacgg
     6241 catcggcgac ccggccagac gtcctgtgcg cctgaaccgc tggtggggcg gcgtgccgga
     6301 atcgaaccgc gtgggcaccc acgagttcat ggacctggcc gagcagatcg gtgcggaggt
     6361 ggtcatcgcc gggaacatgg ggtcgatgtc gccgcgcgac atgagccagt ggatggagta
     6421 catgacggcc accggcccgt cggccttgac ggacgagcgc cgtcgcaacg ggcgcgacaa
     6481 gccatggaag gtcaagtaca tcgggatcgg caacgagagc tggggctgcg gcggaaacat
     6541 gcggcccgaa taccaggccg acctgcacaa ccgctacgcc acgttcttga acggcggggc
     6601 gctgcgcgtc gcctccggcg acggcacgag caacgaccac ctgatcgacg tgctgatgga
     6661 gcgctcgatc gaccagatgg acgcgctgac cctgcactac tacaccctgc ccggcgactg
     6721 gaagaagaaa ggggcggcca ccggcttcga cacggccgaa tgggcccaca cgctcaaggt
     6781 cgcgcgcggc atcgaagccc gcatcacggc catcgaggcc atcatggaca agcgcgatcc
     6841 caagcgccgg gtcgggctgt tcatcgacga atggggcacg tggtacgaca ccgagcccgg
     6901 ggcgaatccc ggcttcctgt accagcagaa caccctgcgc gatgccctcg tggccgcatt
     6961 gacgctcgac gtgttccacc gtcacaccga tcgcgtgcgc atgaccagca tcgcgcagat
     7021 ggtcaacgtc ctgcaggcca tggtgctggc cgatggctcc cgcatggtgt tgacgccgac
     7081 ctatcacgtg tacgacatgt acaagccctt caagggcgcg acgccgctgg atagccgtct
     7141 ggacacgagc aagtggcatc gcggcgacga tgagctgccg gcggtcgatg ccagcgtcgc
     7201 tcgcaccccg gacggcgcca cggtgctcgc catcgtcaat ctcgatcccg agcggccggc
     7261 gcatgtccgc accaacctgc agggcgactt tcgcggaagc atgctgaccg ccgcgaagat
     7321 ggatgcgcac aacacgttcg accagccgca tgccatcgag cccgcgccct tcgcagccca
     7381 cggcggcgcc gccggattga cgctggatct gcctcccaag acggtgctcg tcgtcagcgc
     7441 gccggcccga cactgaaccg cgaccggcaa cgccatggcc cgacattccc tcacgaaatt
     7501 ccccgccccg gccctgctcc tcgccgcggc cgcgctcctg gccggctgcg ccgccactcc
     7561 ggaggccgcc cgtccgctcg cgacggcagc ggcgccctcc agcatggcgg aactcacgac
     7621 gcagatgacg cctggcatca acctgggcaa cacgatggaa gccctgccca acgagacggc
     7681 atggggcaat ccggtgccgt cgcaggccct gatggacggc tacagggccg cagggttccg
     7741 gagcgttcgc atcccgctgg cctggcgtcc ctacgaggat tcgcgacacc agatagacca
     7801 caagtggatg gcccacgtga gggaggtcgt cgaccaggcg cgacgggccg gtctgtacgt
     7861 gatgatcaac acccactggg acgggggctg gatgaaccac ccgacctacg atcaccaggc
     7921 ggccatccag gcgcaactgg ccgcctactg gagccagatc gcgaccgagt tcaaggacta
     7981 cgacgagcac ctgctgttcg ccggcagcaa cgaggtcggg gtggaaaact ccgtggcacc
     8041 gccgacgccc gagcaggcgg ccgtccagaa cgcgttcaac cagaccttcg tcgacaccgt
     8101 gcgcgccgcg ggcgggcgca atgccacgcg ctacctcgtc gtccagagct tcagcacgaa
     8161 catcggtcac gcgatccagt tctcgacgat tcccagggac ccggtgcccg gtcgactgct
     8221 gatggaagtg cactactacg acccgttcaa cttcaccatc aacggcaaga gcaccgtatg
     8281 gcagtggggc aagcgggcga ccgatcgcgc cgcgtccgat ccctgggccg acgaaccctg
     8341 ggtcgacaag cagttcgact cgatgaagac ccactatgtc gaccacggcg tcggggtgat
     8401 cgtcggggag tacggcgcct atgcgaagcc ggaatttccg ggctccgcgc cgtacatcag
     8461 ggactgggcg cgctacgtca ccggcgccat tcgccgcaac ggcctggtgc cgatgtggtg
     8521 ggacaccggc gcgctggtcg accgccgcac cggtgcccag aaggatcccg aactgatcca
     8581 ggcgatcgtc ggcgcctcgc gctgaacagg atgcctcgcg tgccgcacgc gccttcaggc
     8641 cgccatctcg atggacgcct cgttgtccag tgtcgccatg agcggacgag tcagtcgata
     8701 gcgcacgagc gccgcggctc ccaccgcgac cgcgtccctg ccgaatcggg aactccgcac
     8761 gttcggcgcg ggaaggttgg cggcggccgc atactcggtc aacgtgcgca gggccggctg
     8821 gagaaagacc tcgccgagct cggccatctg cccgccgatc acgatggagc cgggatcgta
     8881 ggcggacgcc aggttctgga gcagcacgcc caggtagcct ccggccttct tgaccgcacg
     8941 caaggtttcc gggtgccgct cctggaccag acgccggatc tcgcgcagcg gatgcggatg
     9001 gtcctcgggc gcccccagga tggagcgcgg accgatcagc gcctccgcgc acccgcggcg
     9061 accgcacgag cacatcggcc cgttgatgtg cagcaccatg tggccgactt cgcccgcgaa
     9121 gccgcgccgg cccgtgaaca gccgatcgct gaagatcacg ccggcgccga cgccctggtt
     9181 catgctcagg tagagcaacg gatccgaggc gatcgaagga ttgaattcca tctcgcccag
     9241 cgcggcgacg tccgcttcgt tctgaacgaa cagcggcacg cccgagagcg cggtgcccgc
     9301 gagcttctcc ctgagcatcg ctccgaccgg cacgtcgcgc cagccgaggt tcggcgcgaa
     9361 gtgcaggaag cccgtgtcct cgtcgacgcc gccgggcagg cccacaccga tgccgacgac
     9421 gcgctgccgg ctcgaattga gctgcgcccg gacgttcagc atggcattgg ccaggccgcc
     9481 gatgcaggcc ttggccgact tgctggagcc gaagttcgtc gtcgcgctgg ccaggatgtc
     9541 gccggtcagg ctcgtcgcca cgacgcgggc cgactcgatg tcgacttccg cgccgatcaa
     9601 cagcaggcgc tcctggtcga tgaacatcgg cgtcggccgc cgtcccaggt cacccgtcgc
     9661 gacgacctcg cgctcgcaca tccagccttc ctcgatgagt tcgcgcacga tggtgctgac
     9721 ggtcgacttg gtcagcccca ccgctgccgc caggtccgcc cgggacatgc ccgggtgcgc
     9781 gaacagctgg cgcaccagcg acatgcggtt gatgtccttg agcaattgct ggtgtccagc
     9841 ggagtcggct gtcacggctc gttcccttgg tttgttcttg aacgcgagtg tcgtcgacct
     9901 cgagccgcca cggcgggcaa tccggaggaa gatcccgaat cattttagtt cggtcgttgc
     9961 acgaatgaaa cggagtttac acctagcagg gggctgagcc tgcgctaggt ccccagcgcg
    10021 gattttcgcg gattccggcc ctggtgccag tctccagcgt ggtcgcagcc actaggtcgt
    10081 cctctgcggt gccaccagac aatgcgagct gcaccttgcg caacgagtca gcggccgatt
    10141 ccaccggcgg aatgaacgga ctcgatgcgg ccgcgacgcg cgacgaggcc gcagacgccg
    10201 cggcgggcgc ggcgagccgc cggcgcgcgc ggctggttcc gcttccctgc gcggcggtcg
    10261 ccttgggatc gcgttgctgc atcttggcct tcgccgctgg atgaacgtta ccgcgcggcc
    10321 atgacgatct cgagcaccgt gaccggttgc ccgcggtact gcggctccac ggacaccgcc
    10381 aggccgtccg ggccactgcg ccaggcaacg ccttcgccgg agaggtttcg caaggaagtc
    10441 acgcctgctg gcatgggcgg cagcaccagt tcggccgtcg gccaggcgtg cacgaacaga
    10501 taggcccggt cgccctgctg cgtcatgccc cacagcccgt ccacaggctg cagcgggccc
    10561 ggccgggtgc cgtagacggc gtggccgtaa cgctcgagcc agcggccgac ctcgcgcagc
    10621 gtccgcgcct gcatcggcgg gatgacaccg tcggcgtccg gaccgacgtt ggccagcacg
    10681 ttgccgttgc gaaccacggc atcgacgagg agggtcacga cctcgtcggc gctcatcacg
    10741 tgcacttccg gcacgtagcc ccaggcgctg ttgaggctga aggtcttctc ccacggcctg
    10801 ggcctgatgg gccccttgat cggcgccggc ccttcctcct cgtcatagtc gccctgccag
    10861 cccgagcggg ggttgatgac gatcctgggc tggtaccgcc ggaccatccg gttgagcttg
    10921 tcgggctccc agaaccaggc ggcgtccgcg tcggtgccct ggtgagccag ccagccgccg
    10981 tcgtaccaga ggatgtcgat gggcccgtag ttgcgcatca actcctcgac ctgcccgtag
    11041 gtctgctgct tgaacaggcg ggcgttgtcc ggcatctccc ggggatggaa ataggccgga
    11101 aagcgccagt ccaggggcga gtagtagaag cccacccgca gtccatggcg cctgacggcc
    11161 cgcacccacg gggcgatgag gtcgcgtcgc gcggccgaat gcggtgcgtc gaagtggccg
    11221 tagctggcct ggctcgcgaa cagcgagaag ccgtcgtggt ggcgcgtggt catgaccatg
    11281 taccgcgccc cggcctggct cgccagcgcc gcccaggcgt ccggatcgta gtggcgggga
    11341 ctgaagcgcc gcgccagcgc cgcatactcg tccggcggca ccttgtcgtt gaacatgtgc
    11401 cattcgcccc gggcggcaat ggcatagagg ccccagtgga cgaagacgcc gaacttcgcg
    11461 tcgcgccacc agtccaggtc ctgcggcggc agcaacaggt ccggcgcgag cgaggccggg
    11521 gcgttcgcgc ctttcagggc ggaccgctgc tcgtcggtca cgccgtccgg cgcggcggcc
    11581 accgcggccc gtcgcggcag cagcggagcg acagccgccg cgcacagaag ggaacgccgg
    11641 ctgaccttgc cggcccggcc accgtcgctc atgtgttccg cctctcaaag cgggacgccg
    11701 acgagcggcg gtcgtcgatg cggccgccct cctccgtgcg ccggcatggc ggcccatcgg
    11761 catggcggca agagcaagcc atcgcgttcc cgttgcgcag ggccgacatt cagaactcgt
    11821 accgcgtgct gagccggaag ctgcggccgt attcgtacca ggcgcggccc atgtagcccg
    11881 gggcctgctg ctgggtctgg cggcccatgg tgttggtcag gttggtcacg ctgagggaca
    11941 ccagccagcg atcgcccagc atcttgtagt cgacaccggc gtcccactgc cccagccctt
    12001 ccagccagac cggcaggccc cagcccacgt cttgcgcacc gggccgcgcc ggatcggcgc
    12061 tggtggcgtt ggtgcccgag gtgctgttgt tgttcacgcc ctgcaggtag cgcgaacgcc
    12121 agttgtaggc caggcgcgcc gacagcggac cgtggtcata caggaacgcc aggttgaagg
    12181 catccctgga caggtactgg atcggcaatg acttgaacgg caggccgttg gtgtcgcatc
    12241 catagacgtt caggttggaa ttgttgaacg cactggcggc cgggcagtac ttcaggtcga
    12301 acgggtggta gagctgctgg tggctgtcga tgtaggtgaa gttcgccgag atgccgaagc
    12361 ccttggccca atccggcagc agccgctcca gggcagcgaa cttgtccagg taggtcatgc
    12421 cgcccacttc gaaaccggcg acggagccct tggcgaggtt gtcgggtccg gtgacggtga
    12481 agtcctgcag gttgcccgcc aggtcggcgt agcgctcgat gtgcgccccc ggaaggatga
    12541 tgtcgcgcag ctgcttgtag aacaccgcgg cggtcaggga ctggccccag gtgggattcc
    12601 actccaggct caggtcgagg ttgttcgagc ggatcggctt gaggcgcgga ttgccgttcg
    12661 agttgccggt gtacgtgatg ctggtgatcg ccccgttgtt gttggtcgag acgttctggt
    12721 tcagcgtttc gtagccctgc aactgcccga agtccggtcg gtagatgccc ttggacgccg
    12781 cgaaccggcc ctgcagcttg ctggtcaggt cggtcttcac gttcaggctg ggcaggaagt
    12841 cggtgtagct ggtcacgagg gtgtgcgccg cgttgatctg gccgaattgc ggcacttcag
    12901 gcggcggatt cgtgcccggc gtgaacaccg tgtaggcgtg cgagatggtg cgggtatgga
    12961 ccacgcgcag gccgacattg ccttccaccg ggaagcgcca gccgtcctcc gcgaagcgca
    13021 gggtgccgta gagcgcctgg ctctgttcgt cggtacggct gacctgggcc gggttgccgt
    13081 cgtagctgag cggcgtccac gtcggcggct ggcaggtgtt gctggagccg aattgcgcgt
    13141 tgccgtcctt gcattcctgg accaggatgt ccgtcagcgg ctggtagctg ttcggatagc
    13201 ccccaggcaa ggacatgctg ggaaacacca gcgcgggcag ctgcgcgacc ttgccattga
    13261 agaagttcgg gaaggactgc acctgggtcg gcaccaggtt ggcgtacacc gacccgggct
    13321 tcaggtagcc gacgttggcg aacgtgcccc acgagggctg gtcgctgttg ttgggcagcg
    13381 tgcccggcat ctgggtctgc cgcacgttcc acggatccga gaagctgaac cagcccgtgc
    13441 cgtcggcgtt ggtgtacttc gcgctgcggt tggtcacgcg catgcccagg cggaagtcac
    13501 gcagcacgtc gctgtcgaac ttgaaccggg catcgccctt ccacgcgacc atgttgccgg
    13561 tggccttgct ctgcatgggc atctcgttgt ccatgtagta gttcgccgga tttgccagga
    13621 aggcgactgt cgacggattg aaggtcaacg agggcgggcc gccatgggcg gtgctcaggg
    13681 tcagctccgg gacgaaggtg ccgatggccg cgtagttgtt gaagctgtcg tcgctggcat
    13741 gcacccattg caggtcgttc tgcagcgcca ggtgatcgct gacgtcccac ttgaagttcc
    13801 acgcgatgtc gcgtgtctgc gaatggctca ccgaacgacc gacgtcggtg tcgtagctga
    13861 ggccgccggc cacgaattcg ttggaaccca gctgccccat cggcacggtg tacgtcccgg
    13921 acgtcagcac gttgccgcta tacaccgcgc tctgggggtc gacctggatc aggtacggat
    13981 tgatgccatt ggcgtgcagc gcgtactccg cgctcgtgtc cttgtagaac gagttgaaga
    14041 tcgtcagcga cgactccttg tcgtcgttct tccactgcaa cgccgcatag aggcccgccc
    14101 ggttgtcctg gctggtgttg gtgcgccagg aggccgactg cggaatccag accgtcttgc
    14161 ccgccaccag gtcgttgcgc gggaaatagg cgtccagttc gatggtgtcg ttgtgcgtgt
    14221 tggtctggtt gaacgacagg tcgaccagcg cgccgaactg gccgacgctg gagtcccagc
    14281 gccggctgta gatccccgag acgcccggcg acttcttgct gcccatgacg gtgtagttgt
    14341 cattggccga gatcgagaac ttggtctcct tgaagtcgaa cggcatcgcg gtgcgcaggt
    14401 cgacctgccc gctgatgcca ccttcgatca gctccgagga aggattcttg tacaccagca
    14461 ccgccgccat caactccggc ggcacgtcgc cccagctcag cgcgcggccg gggtatccgg
    14521 cggagaagga ttcgcggcca ttgagcgtcg acgagcccca ggacaggccg cgcacctgga
    14581 cgcccgagcc ttcgaccgaa aagtgttcgg ggtcgcccgg cgacttgttg cggtccatcg
    14641 tcacgccgac gacgcgctgc aggacctcgg tgatcgactt gtccggcagc ttgcccgctt
    14701 cttccgccac cacggcatcg acgatctgct cggcgttctt cttgatggcc tgggcgcttt
    14761 gcagagccgc acgctggccg gtcacgacca cctgctgcga atcccggtcc gcttccgccg
    14821 gtgcggcctt ggccgcgttc gccgaggccg cggccgccgg cgacgaggcc gaagcggccg
    14881 gcgcggaagg cgcgtcgcct ggcgcgggcg ccgccgcctg cgccagcgcg gacccgctgc
    14941 aaagcagaag gacctgcgcg gccgccacgg aaagcgccgt cttgctgaaa tgtctcctca
    15001 ttgtctcttc cctcttgaat gagccgctgg cccagccctc gatggagcgg gtcggaaatg
    15061 gtgtgaccga agaccggtcg gatgtgcatc ggtgcgcggc acggtggccg tcgccgggcc
    15121 gttggcgacg gtgccacgcc cttcatcgcg cccaaggcgt ggtggcgttc gtcgctggcg
    15181 cgccatccac tcgggacgac gttcgtggcg tcgtcccggt ggcggcactc actggatcag
    15241 gatcggagtc cccacgctga tgaccgtcgc gtatgcgggg ctgtacggcg tgtaggggta
    15301 gtgctgggcg ccctggttga ccggcgccgt gccgttcacg ctcgcattgg tgctggccgg
    15361 tgagaactgc acctggacga tcggatagct cgtcagctcc gtggcggcca ccagcccgga
    15421 caggccgcag ttggtcgaga cgacgttgaa gttcgacaag gacacctggt aggccgcctg
    15481 ggccgccgcg gtgggcgtca cgctcgcctg gaccttgacg ttgcagggct tgtcctggaa
    15541 ctgattgaaa tgtcccagcg tcagcgtgac cagcacgtcc gcgccatgcg gactgcccgg
    15601 actcagcagg ctgaaccatt ccgggttctc gccgagcgtg aacgacaggg tggtctggtt
    15661 ggccacctgc aggccctcgg ccgtgttgcc ggtctggctg atgccgttcg tcaggcccgg
    15721 gacgttgatc gtgatgccgt tgtcgtaggc gtagatcggc gtggtctgca ccgccgccgg
    15781 atcggtgggc tgcaacacga aggtgtaggt gaggacgctg ttgtccgggc tcagtgcgct
    15841 ccagcaccac tggtcgttgg agcaccacca gttgtcgatg ttggcgccgg caccggtgcc
    15901 gatcgagccg ccgtcggtgg tcgcggaggt gccctggaag ccggatgcca ccgtcagcgt
    15961 gggcggcggg gcgtcgcctg cgttgacggt gacgtccacc ggcgccgccg cggcatagtt
    16021 ggcgtcgccg ggctgcgtgg cttcgatcgt gcactgcccg ctgccggcgg ccatcgcggt
    16081 gttgccgctg acggtgcagg acgtcggcgt gagcgaggtg taggtcaccg ccagccccga
    16141 gtcggaggtc gcggtcagcg tggtcggctg cgcgccaagg gccaggaagc ccggcgacgg
    16201 gaaggtgatg gcctgcgggt ggggaagcac cttgaacagc tgctgcgtcg gtgcggcggc
    16261 tgcgaagctg ccgtcgccgt cctgggtcgc ggtcacctgg cattcaccca ccgcgaccgg
    16321 cacgagcttg ccgtcgctga tcgtgcagac cgacggagtg ttcgacgtca gcgtgacggt
    16381 caggccggac gtcgccgacg cggaaagcgg cgccgccgcg gtcgcgaggt atcgagtgcc
    16441 cggatacgga aagtcgatgg actgggcagt tcccccggag gagccgccgt cgccgccgca
    16501 ggcggacaga agtgcgccga aggcaacgac gaccgtcgaa gccttgctga gcatggtcgc
    16561 ttgtctcatg tcttgtccta tttatttgtg gcgattgccc gcattcaaag gtctgcgtcg
    16621 cttgctgccc aagacagcat cgacgccatc gatcgcgtcg cggttcggga tttcatcggt
    16681 cgaccgaggg gttgcttcgg ccttggacga gcgctccgga actcgcacag acgcatggag
    16741 atccgtccgg acttcggcat accgagcccg cgacgaagcg cccctggcac ccgcacctgc
    16801 gatccgcggt tcgggcacgc aaccgacatg accctcgcat cgtctgccgg catgtcattg
    16861 cgtctgcctg gaaatactag ttcgggcgat gaccgaacaa atatcgtaca aacacctacc
    16921 ggcgtcgggg ccttggccga cagcgagcgt gggccgacct cgacgaagcg cgtcgatgca
    16981 tctggtccgc ctgccgcgtt gccgattagc cgatcaccgt cctcatcttg ctgcctcccg
    17041 cgaaaaaggc gccggacgcg gcgtggtccg ctttggaacg catcgtccgg cacaggagac
    17101 gcgtcgatac taggcagcgg cgcacgcgat gtcataacca agctatgacg ctcgcgaaac
    17161 gggacgcgga cgcgcagtcc tccacggaaa cgtgcacgtc cggtccatcg cgttccggca
    17221 cgcggcccgc ccgattcgat caagggtaaa caccgcactg tttgttcatc gaccaaatta
    17281 atatgcgact gaaacgtcac atcaccgaca agatcgttgt tacttgattt agcgctacat
    17341 ctgatccgcg caccgcggtt cgatgcagcg cgatcgagat cgcgcgatcg atgtcgcacg
    17401 gcgattcgga aagcccggcg cggcgcctgt cgccggctga attcaccgag gttcccatgc
    17461 tcgaactttc ccaaggtgcc tactccaaga acgcccccgt tcgggtcgac gcccgtgcag
    17521 acttgccgct ccggccgtcc ccggaaaccg gcctgcgctt cggacgctgc gaagtccggc
    17581 cctcgcggcg cgaactgcgc gtggacggac aactgcggca gatccagccg cgcccgttcg
    17641 acctgctcgt ctacctgatc gaccatcgtg atcgcgtgct gtcgatggac gagctcctgg
    17701 acgtcgtctg gggccagcgg atcgtgcagc ccagttcgct caccgtggcc atcaaccgga
    17761 tccgcagcgt gctgggcgac gagcgcggcg agatcatccg cactcaccat cgcgtgggct
    17821 atcgattcgt ggcagagctg aaggccgatg agctgcactt cgcgccacgg cagcaagccg
    17881 ccatcgcctg aatggcaacg cgcggaatac tcgtcgacca ccggctgatc ttgctgctcg
    17941 cagcgcttgc gactgcgtgc accgcgccgc agccaggcgg gaccgtgagc cccgcgcagg
    18001 cgcgcgcgaa cttcgcgcgc cccgtcgtgc tggcggccga cgatgtgcgt gcgtacccgg
    18061 tcccgccgcg cggcttcgcc gacccgaagc cgggcgtggc gcccggtcgc atcgaagacc
    18121 tcagctatct gtcggcggtc acgggcacgc ggcgtcaggc ccggatctac ctgcccgcgg
    18181 gctaccaggc aagccggagg tatccggtgc tctacctgct gcacggcatc gcgggcaacc
    18241 aggacgagtg gcgcggctat gtgcgcgcgc cgaccatcct ggacaatctc caggcggccg
    18301 gccggatggt gccgatgatc gtcgtcatgc ccaacggtcg cgcgttggcc gacgatgccc
    18361 cgccgcccgc cgacaaggtg ttcacgccgg ccaatgcggc cggcttcgac aggttcgaac
    18421 gcgacctgct ggacttcctg gtccccgcgg tcgattccac ctacccgacg ctggccgaca
    18481 gcgggcatcg cgccatcgcc gggctctcga tgggtggcgg ccaggccctg aacttcggac
    18541 tggcccatct cgaccggttc gcatgggtgg gcgccttttc cccggcgccc aacacccgcg
    18601 ggatcgccga cttgattccg gatcccgtgg atgcgcgacg cgggctcagg ctgctgtacc
    18661 tgtcgtgcgg cgaccaggac ggcctgatca acgtgtcgca ggacgtgcac cgcggcctgg
    18721 cgcagcgcgc cgttcctcat ctgtggaacg tcgacgacca cggtcatgac cgcgacagct
    18781 gggccgagaa cctctacaac ttcgcgcagc tgatcttccg ttgagccctg gggcaccagg
    18841 agcatcagtc gcccccgggc tccagcacga cgcggccgac gatgcctgag tcctgccccg
    18901 gctgcgactg caccaggaca ttgagcgtgc gccatgccgg cccctgcggc agttgcagcg
    18961 tcagcggccc cggggcggga tccgtcttct cgcccagctt gacaccttcg caccagacct
    19021 cggcgcggcc ggcgatctcg cggaagacga ggcgtccccg accgtcgttg cggtcggcac
    19081 gcacgttcaa cgacgcgcga tagacgcgcc acggctgccc ctccgggccc tgcttcatcg
    19141 gcggctcgcc ccagccccag gtgttcatgt ccccgtagct cggggcggtg ccgggatcgg
    19201 gccggtcgcc ctgcggcggg gagatgcgcc agccgccgat cttggtgaag
//
LOCUS       DATCRO010000969         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_314909, whole genome shotgun sequence.
ACCESSION   DATCRO010000969 DATCRO010000000
VERSION     DATCRO010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314909"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VIN75_26515"
     CDS             <1..1551
                     /locus_tag="VIN75_26515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HEY8977796.1"
                     /translation="SDAEAWVEVTLKLARKAPLPGVDQRPARALTAAELGAQYGASDA
                     AIKRVSDAYANMGLTVLASDAATRRVQLGGPVSAMEQAFQVKLMQYDGIRGPYRGRVG
                     PLHVPAALDKDIVGVFGLDNRRVVRRRPDGGKRRHVVDRKTATTKKRPWFFPAELAAA
                     YDFPDGDGSGQTIGLLEFGGGYFPDDLAAFGQAAGLASLPDVVPISVDNMPTDTNDEA
                     TGEVMLDVEVLAGVCPKATIPVWFSQFTEKGWIDALDAAMHDATYKPQVLSISWGYAE
                     DVSVWTASAVTQVNEALQEAALMGITVCVAAGDDGSSDGINDGHAHVDFPCSSPFVLA
                     VGGTLLRAGAKRSERAWKDGDGLRADNGGSTGGGVSARIARPAWQSGLDIAPVNPGQQ
                     AGRIVPDVAANASANTGYFVVAGGQQEISGGTSASAPLWAALVARMNQQLAASGKRVG
                     YLTPLLYGAASAGGGPLGKAGCNDIASGDNVTAHVGGFKSTSGFDAVTGWGSPRGKDL
                     LAALAKVL"
     gene            1665..2135
                     /locus_tag="VIN75_26520"
     CDS             1665..2135
                     /locus_tag="VIN75_26520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977797.1"
                     /translation="MSLIPFLHATPGLARGAIPLAAAAALAAALPGCSRHAPPPPASA
                     PAAPPADVPQDMAGVWTSTRGAVMRCVELHADGTYMMLPNTEAGDHLNYQGTWRVAGG
                     NITWRDSSQGFSPDVNAMEGVSPGHFATVEADNSRSQFDRIAGPGEKCPSEVSK"
     gene            2132..2725
                     /locus_tag="VIN75_26525"
     CDS             2132..2725
                     /locus_tag="VIN75_26525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8977798.1"
                     /translation="MTVTTAPLNVLALCGSLRKDSLNAALLRAAARLARPGADIEIAD
                     WLGRLPLYNPDLEDVVADDELPRAVVALRLAVAAADAIVIASPEYAHGVSGTIKNTLD
                     WLVSFPPFIHKPVAVLNASPRAHHADDALRETLRTMSAGLVGERSLPVALLGAHMDEA
                     AMAASPEVAGVVAEALAALRAEIERQHVADVPVFPLD"
BASE COUNT          414 a         1141 c         1088 g          403 t
ORIGIN      
        1 tccgatgccg aggcctgggt cgaggtcacg ctcaagctgg cgcgcaaggc gccgttgccg
       61 ggcgtcgacc agcgcccggc gcgcgcgctc accgcggcag agctcggcgc gcagtacggc
      121 gcgtcggatg ccgcgatcaa gcgcgtgtcc gacgcctacg ccaacatggg cctgacggtg
      181 ctcgccagcg acgcggccac gcgcagggtg cagctcggtg gccccgtcag cgcgatggag
      241 caggccttcc aggtcaagct gatgcagtac gacggcatcc gcggcccgta ccgcggccgc
      301 gtcggcccgc tgcacgtgcc ggcggcgctc gacaaggaca tcgtcggcgt cttcggcctg
      361 gacaaccgcc gcgtcgtgcg ccgccgcccg gacggcggca agcgccggca cgtcgtcgac
      421 cgcaagacgg cgacgaccaa gaagcgcccc tggttcttcc ctgccgagct cgccgcggcc
      481 tacgacttcc ccgacggcga cggcagcggc cagacgatcg gcctgctgga attcggcggc
      541 ggctacttcc ccgacgacct cgccgccttc ggccaggccg cgggcctcgc gagcctgccc
      601 gacgtggtgc cgatcagcgt cgacaacatg cctaccgaca ccaacgacga ggccaccggc
      661 gaggtgatgc tggacgtcga ggtgctggcc ggcgtgtgcc cgaaggcgac cattcccgtc
      721 tggttcagcc agttcaccga gaagggctgg atcgacgcgc tcgacgccgc gatgcacgac
      781 gcgacgtaca agccgcaggt gctgtcgatc agctggggct acgccgagga cgtgtcggtg
      841 tggacggcat cggccgtcac gcaggtcaac gaggcgctgc aggaggccgc gctcatgggc
      901 atcaccgtct gcgtggccgc cggcgacgac ggctcgtccg acggcatcaa cgacggccat
      961 gcgcacgtcg acttcccgtg ctccagcccc ttcgtgctgg ccgtgggcgg caccctgctg
     1021 cgcgccggcg ccaagcgcag cgagcgcgcc tggaaggacg gcgacggcct gcgcgccgac
     1081 aacggcggct ccacgggcgg cggcgtcagc gcgcgcatcg cgcggccggc gtggcagtcg
     1141 ggcctggaca tcgcgccggt caacccgggg cagcaggccg ggcgcatcgt gccggacgtc
     1201 gcggccaacg cgagcgccaa cacgggctac ttcgtcgtcg ccggaggcca gcaggagatc
     1261 agcggcggca ccagcgcctc cgcgccgctg tgggcggcgc tggtggcgcg catgaaccag
     1321 cagctggcgg ccagcggcaa gcgcgtgggc tacctcacgc cgctgctgta cggcgcggcc
     1381 tcggccggcg gcgggccgct gggcaaggcc ggctgcaacg acatcgcgtc gggcgacaac
     1441 gtgacggcgc acgtcggcgg cttcaagtcg acctcgggct tcgacgcggt gacgggctgg
     1501 ggctcgcccc gcggcaagga ccttctggcc gcgctcgcca aggtgctctg agtccgcgcg
     1561 ggggcatgcg tgacgcgccc gcgcgcccgg gcgcgaccgc cttagactgg gggcgacggg
     1621 cgccggctgc cgtggccgcg cgcccccgtc cgacggagcc ccacttgtcc ctgatcccgt
     1681 tcctgcatgc gacgcccggc ctggcccgcg gcgcgatccc gctggccgcc gcagccgcgc
     1741 tcgccgcggc gctgcccggc tgttcaaggc atgcgccccc gccgccggca tccgcgcccg
     1801 cggcgccgcc cgccgacgtc ccgcaggaca tggccggcgt ctggacctcg acccgtggcg
     1861 cggtgatgcg ctgcgtcgag ctgcacgccg acggcaccta catgatgctg cccaacaccg
     1921 aggccggcga ccacctgaac taccagggca cctggcgcgt ggccggcggc aacatcacct
     1981 ggcgcgattc gtcgcagggc ttctcgcccg acgtcaatgc gatggagggt gtctcgcccg
     2041 ggcacttcgc caccgtcgag gccgacaact cgcgctccca gttcgaccgc atcgccggcc
     2101 ccggcgagaa atgccccagc gaggtatcga aatgacggtc acgaccgctc ccctgaacgt
     2161 gctggccctg tgcggcagcc tgcgcaagga ctccctgaac gccgcgttgc tgcgcgcggc
     2221 cgcccgcctg gcgcgccccg gcgccgacat cgagatcgcc gactggctgg gacgcctgcc
     2281 gctgtacaac cccgacctcg aggacgtggt ggccgatgac gaactgccgc gcgccgtggt
     2341 ggcgctgcgc ctggccgtcg cggccgccga cgcgatcgtc atcgccagcc ccgagtacgc
     2401 gcatggcgtc agcggcacga tcaagaacac gctggactgg ctcgtgagct tcccgccgtt
     2461 catccacaag ccggtggccg tcctcaacgc gtcgccgcgc gcgcaccacg ccgacgacgc
     2521 gctgcgcgag acgctgcgca cgatgtcggc cggcctcgtc ggcgagcgct cgctgcccgt
     2581 cgcgttgctg ggtgcgcaca tggacgaggc cgcgatggcg gcgtcgcccg aggtggccgg
     2641 cgtcgtggcc gaggcgctgg ccgcgctgcg cgccgagatc gagcgccagc acgtcgcgga
     2701 cgtgccggtg tttcccctcg actagggcct gttcacactc agtgcgtgcc cgcgccggct
     2761 gcctgcgggt gatcctccag aaagcccgcc ttcggcgttg tgaccgcagg gaacgcttgc
     2821 cgtggcgcca ccacgtcggc gcgctcgcgc cttgaaggcg ggctttctgg aggtcacgcg
     2881 ggcacgcatt gagtgtgaac aggccctagg agtctgcgcg gcagaaggcg cctgctgcga
     2941 acggcgataa gcggggcctt ttcagcggcg gctcctctcg cgtagcgccc actacgagtt
     3001 cgtcgccgcc gctgaaaagt ccatgcttct cgccgctctc gcgacg
//
LOCUS       DATCRO010000970         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_415902, whole genome shotgun sequence.
ACCESSION   DATCRO010000970 DATCRO010000000
VERSION     DATCRO010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415902"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1403)
                     /locus_tag="VIN75_26530"
     CDS             complement(228..1403)
                     /locus_tag="VIN75_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY8977799.1"
                     /translation="MTFRFRSAITQPAAALNFAVLAVAVLAATPSVTQAYPITPQQRE
                     TANQVAAKGVPLSELAENAPDTYTVKRGDTLWHISGMFLKSPWRWPELWGMNLDQIHN
                     PHLIYPGQLLVLEKIDGMARLKIGNTVGGAGDTVKLSPSIRSESSESAAISSIPMNLI
                     GPFLTDAMIFDENELAKAPRIVAGEEGHLMLGRGNTAYVLGDLGNTRSWQIFREPKPL
                     VDPDTKQILGYEARFVGSADRKQDGEARPGAESGGLSVPATFIITGNREDAQVGDRLA
                     PATVRDFAPFVPHAPANPVNGKVVSLYGDALSAGSNQIVSLNRGARDGLERGTVLALW
                     HEGAITHDKSVQKGALIKLPDERIGSVFVFRVFDRMAYGLVMDTTQPTLPGDRVSQP"
     gene            1702..2214
                     /gene="def"
                     /locus_tag="VIN75_26535"
     CDS             1702..2214
                     /gene="def"
                     /locus_tag="VIN75_26535"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8977800.1"
                     /translation="MHQLQILRYPDPRLYTVAKPVAEVTDEIRALADEMIQVMYASDG
                     VGLAATQVDRHIRLIVMDTSETHDQPRVLINPELVESSDDWVLGEEGCLSVPQVYEKV
                     ERHARVRVKALDRDGKPYEFDAEGLAAVCVQHEMDHLMGKVFVDYLSPLKRDRIKTKM
                     IKKTREETKR"
     gene            2229..>2514
                     /locus_tag="VIN75_26540"
     CDS             2229..>2514
                     /locus_tag="VIN75_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8977801.1"
                     /translation="MRVAFAGTPDFAAVALKAIAGAGHDVPLALTQPDRPAGRGMKLT
                     PSPVKQLALELGVAVAQPRSLRLDGKFPDDAQAGRAALDAARPDVLVVAAY"
BASE COUNT          408 a          829 c          893 g          384 t
ORIGIN      
        1 gcgctcgggc gagccgaaag ccgtcaggag ccggcgcgcg gtggcgcggc cgacgcccgg
       61 ggtggtcgac agccgcagcc aaccggcgag ttcgtcgcgg gacaacatga gcgcaccggg
      121 cagtcggcgg tcaggggcgt gacgacggcc gtgcgaaggg acgtgcggcg accgcgcggc
      181 gttcgaaccg gaggctcggt gcgttcaggg gcggtcgccg gcgccgctca gggctggctg
      241 acgcggtcgc ccggcagcgt gggctgcgtc gtgtccatca cgaggccgta ggccatgcga
      301 tcgaacacgc ggaacacgaa cacggagccg atgcgctcgt cgggcagctt gatcagcgcg
      361 cccttctgca ccgacttgtc gtgcgtgatc gcgccctcgt gccacagcgc cagcacggtg
      421 ccgcgctcga ggccgtcgcg cgcgccgcga ttcagcgaca cgatctggtt cgagccggcc
      481 gacagggcgt cgccgtagag cgagacgacc ttgccgttga ccgggttggc cggcgcatgc
      541 ggcacgaacg gggcgaagtc gcgcaccgtg gcgggcgcca ggcggtcgcc gacctgcgcg
      601 tcctcgcggt tgcccgtgat gatgaaggtg gccggcacgc tcaggccgcc cgactccgcg
      661 cccggacggg cctcgccgtc ctgcttgcgg tcggccgagc cgacgaagcg cgcctcgtag
      721 ccgaggatct gcttggtgtc cggatcgacc agcggcttgg gctcgcggaa gatctgccag
      781 ctgcgcgtgt tgcccaggtc gcccagcacg tacgcggtgt tgccgcggcc gagcatcagg
      841 tggccttcct cgccggcgac gatgcgcggc gccttggcca gctcgttctc gtcgaagatc
      901 atcgcgtcgg tcaggaacgg cccgatcaga ttcatcggga tcgacgagat cgccgccgat
      961 tcggacgatt cgctgcgaat cgacggcgac agtttgacgg tgtcgcccgc gccgccgacg
     1021 gtgttgccga ttttcaggcg ggccatgccg tcgattttct cgagcaccag caactggccg
     1081 ggatagatca ggtgcgggtt gtgaatctgg tcgagattca ttccccacag ctcgggccag
     1141 cgccacgggc ttttgaggaa catgccggaa atgtgccaaa gcgtatcgcc gcgcttgacc
     1201 gtatacgtgt caggcgcgtt ttcggccagc tccgacagcg gcacgccctt ggcggcgacc
     1261 tggttcgcgg tctcgcgctg ctgcggcgtg atggggtagg cctgcgtcac ggacggcgtg
     1321 gccgccagca ccgccaccgc gagcacggcg aagttcaggg ccgcagcggg ctgcgtgatg
     1381 gcggaacgga atcgaaatgt catgtgcaga aaactcccgg gccgtcgaag gcaatgggac
     1441 gcaggtgaga cggaatccgc gcagaactgc gacaatcccg aatcggccgg agtaatccag
     1501 ccggcaagaa actagcaaat ttgccgcaga ttctgcccgc aaacccctga tgtcgcaatg
     1561 aatcggacac tccgttacga cggagatagg cgttcaccct gagtccggtt gttgcgtcca
     1621 acacattctg caccgcgctc gcaagggtcc tcgccgcatt actcgtgatt ttcgtggcag
     1681 atttccagga acgatctttc catgcatcaa ctccagattc ttcgttaccc cgatccgcgg
     1741 ctgtacacgg tggccaagcc ggtcgccgag gtcaccgacg agatccgcgc gctcgccgac
     1801 gagatgatcc aggtcatgta cgcgtccgac ggcgtcggcc tggcggcgac ccaggtggac
     1861 cgccacatcc ggctgatcgt gatggacacc tccgagacgc acgaccagcc gcgcgtgctg
     1921 atcaaccccg agctggtcga gagctcggac gactgggtgc tgggcgagga gggctgcctg
     1981 tcggtgccgc aggtctacga gaaggtggag cgccacgccc gcgtgcgcgt gaaggcgctc
     2041 gaccgcgacg gcaagccgta cgagttcgac gccgaaggcc tggccgcggt gtgcgtgcag
     2101 cacgagatgg atcacctgat gggcaaggtg ttcgtcgact acctcagccc gctcaagcgc
     2161 gaccgcatca agaccaagat gatcaagaag acgcgcgagg agacgaagcg ctgatggcga
     2221 cgcccgtgct gcgcgtcgcc ttcgcgggga cgccggactt cgcggccgtc gcgctgaagg
     2281 cgatcgccgg ggccggccac gacgtgccgc tggcgctgac gcagcccgac cgcccggccg
     2341 gccgcggcat gaagctgacg ccgtcgccgg tcaagcagct ggcgctggag ctgggcgtgg
     2401 cggtcgcgca gccgcgcagc ctgcggctgg acggcaagtt ccccgacgac gcgcaggccg
     2461 gtcgcgccgc gctcgacgcc gcgcggcccg acgtgctggt ggtcgccgcc tacg
//
LOCUS       DATCRO010000971         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_247444, whole genome shotgun sequence.
ACCESSION   DATCRO010000971 DATCRO010000000
VERSION     DATCRO010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247444"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2111
                     /locus_tag="VIN75_26545"
     CDS             <1..>2111
                     /locus_tag="VIN75_26545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977802.1"
                     /translation="LERRVPGIASHAGDAQGRRGPRRALVFPLRWADDKPPAGALVAG
                     LNPLRPLDGDYRGFVRLVAQQVGASLAQARALQLEREQRERLAELDRAKTEFFANVSH
                     EFRTPLTLLLSPLDELQRRSNELPAGLATEIDVAARNSRRLLQLVDDLLDFSQIEQRR
                     QRAVLQPTDLASLTTDVASVFRSAIERAGLAMRLECADDLPLVPVDRDMWDKIVSNLL
                     SNALKFTFDGAITVRLGARSLHVELVVSDTGVGIPKGELPNLFKRFHRVRGARARTIE
                     GSGIGLALVHDLVSRMGGQVQVRSIEGHGTDFTVWLPLKPFRRTMDAVADDATDARAA
                     PIAAGLAGEAGNWGDERVPRDMVDDPLGAPKGGRLRPAPGAHVLVADDNADLRDYLRR
                     LLGAYWTVTLAADGAQAIREARSQSPDLILADVMMPNVDGFALLRAVRGDAALKDTPV
                     IFLTARAGEDAAIEGLLAGADDYLAKPFSARELIARVGGQIELSRARRRTEELNALLV
                     RFSDAVRGARGPAAVAETACRLVAEHLRVERACWAEIDWTARDYVIGAAFERAGVPAV
                     AGRFGLDAWEPFTTSLLEGKPVVVDDTQSDPRVPSLIREGYARLGVGADVAMPVLVNG
                     RFVSLLAVNQRETRRWSAEELALVAGIAGRCWSEVERARGAAALRESEAHLAEELEDA
                     RVLQKMSNQLVSEAGPNGHYN"
BASE COUNT          294 a          758 c          769 g          290 t
ORIGIN      
        1 ctcgagcgcc gagtgccggg catcgcgtcg catgccggcg acgcgcaggg ccggcgcggg
       61 ccgcgaaggg ccctggtctt tccgctgcgc tgggccgacg acaagccgcc ggccggtgcg
      121 ctcgtcgccg gcctgaaccc gctgcgcccg ctggacggtg actaccgcgg cttcgtccgg
      181 ctggtggccc agcaggtcgg cgcgagcctg gcccaggcgc gtgccctgca gctcgaacga
      241 gaacaacgcg aacgtcttgc cgagctcgac cgggccaaga ccgagttctt cgccaatgtc
      301 agccacgaat tccgcacgcc gctgaccttg ctgctgtcgc cgctcgacga actgcagcgc
      361 cgcagcaacg agctgcccgc cgggctcgcg accgagatcg acgtcgcggc gcgcaactcg
      421 cggcgcctgc tccagctcgt cgacgacctc ctcgacttct cgcagatcga acagcgccgg
      481 cagcgcgcag tgctgcaacc cacggacctg gcgagcctga cgaccgacgt cgcgagcgtc
      541 tttcgcagcg ccatcgagcg ggccgggctg gccatgcgcc tggaatgcgc ggacgacctg
      601 ccgctcgttc cggtcgaccg cgacatgtgg gacaagatcg tctccaacct gctgtccaac
      661 gcgctcaagt tcaccttcga cggcgcgatc accgtgcgcc tgggcgcccg ctcgctgcac
      721 gtcgaactcg tcgtctcgga caccggcgtc ggcatcccga aaggcgagtt gcccaacctg
      781 ttcaagcgct tccatcgcgt gcgcggcgcg cgggcccgca cgatcgaggg ctcgggcatc
      841 gggctggcgc tcgtgcacga cctcgtctcc cgcatgggcg gccaggtgca ggtgcgcagc
      901 atcgagggcc acggcaccga tttcacggtc tggttgccgc tgaaaccgtt ccgccggacc
      961 atggacgccg tggcggacga cgccaccgat gcgcgcgccg cacccatcgc ggcgggcctg
     1021 gccggagagg ccggcaactg gggcgacgag cgcgtgccgc gcgacatggt cgacgacccg
     1081 ctcggcgcgc ccaagggcgg gcggctgcgg ccggcgccgg gcgcgcacgt gctggtcgcg
     1141 gacgacaacg cggacctgcg cgactacctt cgccgcctgc tcggcgccta ttggacggtg
     1201 accttggccg cggacggtgc ccaagccatc cgggaggcgc gcagccagtc gcccgacctg
     1261 atcctggccg acgtcatgat gcccaatgtc gacggcttcg cgctgctgcg cgccgtccgc
     1321 ggcgacgcgg cgctcaagga cacgccggtc atcttcctga cggcgcgcgc gggcgaggac
     1381 gccgcgatcg agggcctgct cgcgggcgcg gacgactacc tggccaagcc gttctcggcg
     1441 cgcgagctga tcgcgcgggt cggcgggcag atcgagttgt cccgcgcgcg gcggcggacg
     1501 gaggagctca acgccttgct ggtgcgcttc tcggatgccg tgcgcggcgc ccgcggccct
     1561 gcggccgtgg cggagacggc gtgccggctc gtggccgagc acctccgcgt ggagcgggcc
     1621 tgctgggcgg agatcgactg gacggcgcgc gactacgtga tcggcgcggc gttcgagcgc
     1681 gccggggtgc cggcggtcgc gggccgcttc ggcctggacg cctgggagcc gttcacgacg
     1741 tcgctgctcg aaggcaagcc ggtcgtggtc gacgacacgc aatcggatcc acgcgtgccg
     1801 tcactcatcc gcgagggcta cgcaaggctc ggggtgggcg ccgacgtggc gatgccggtc
     1861 ctcgtcaacg gacggttcgt gagcctgctg gcggtcaacc agcgcgagac gcgccgttgg
     1921 tcggccgagg aactcgcgct cgtcgcaggc atcgccggcc gctgctggtc cgaggtcgag
     1981 cgcgcccgcg gcgcggcggc gctgcgcgaa agcgaggcgc atctggcaga ggaactggag
     2041 gatgcacgag tgcttcagaa gatgagcaac cagctcgttt ccgaagcggg ccccaacggg
     2101 cattacaacc a
//
LOCUS       DATCRO010000972         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_708540, whole genome shotgun sequence.
ACCESSION   DATCRO010000972 DATCRO010000000
VERSION     DATCRO010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708540"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VIN75_26550"
     CDS             <1..779
                     /locus_tag="VIN75_26550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977803.1"
                     /translation="DLPMPPGLRAIARRPYAWWTDRLLARRDSPVMARLLVGLGPATH
                     WTTRIRDGVLFVLVGGGLCAVVSALAGREFGAMVAPWLSFSLLTGACTPALQAVARLH
                     RTRSEQALLVLLPGVPRGAGLNRWLAWRMSLVFVVAALCGFALAAALNAFAEAIQPGV
                     VNRSTGGMAFALFPALLPQVAWQWRRWARLRGVTGLEMSMPGIAPVLLGLAALMLHLV
                     TGTGYLPIGVAFAAVALAYCAWRWRRMAVEPTALPVGRLA"
     gene            complement(802..2346)
                     /gene="gshA"
                     /locus_tag="VIN75_26555"
     CDS             complement(802..2346)
                     /gene="gshA"
                     /locus_tag="VIN75_26555"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY8977804.1"
                     /translation="MRGLQERLKALSAARLQGIGRGVEKESLRVTPAGALAMTPHPRA
                     LGSALTHPHITTDFSESQLELITGVHRSAESVEAELTQVHQAVYRALGDEMMWVGSMP
                     CTLPPDEDRIPLGQYGTSNVGMSKTVYRTGLGHRYGRRMQTISGIHYNWSLPEVTTAE
                     YFGLIRNFRRRSFLLLYLFGASPAIPRGFADQAPDLQPMGPDTLYMPHATSLRMGRLG
                     YQSDAQNSLCVSYNSLDSYAASLSDALVRPYPAYQKLGIQAEDGSYLQLSDSLLQIEN
                     EFYSTIRPKRVIFPGERPLHALRERGVEYIEVRCMDLDPFVPAGIAPATMRMIDIFLL
                     HCLLDDSPLDSPNEIAELGRNQQRVASRGREPGLKLERGGVEVLMTDWLREILTECAP
                     IAAWLDANEGTDAHVQALAAARAAVLAPETLPSARVLAQMQSQHGGSHDAFVRTQSQR
                     TREHMLALDLPADVAAHMAELATESLAEQERIEAADTMPFEIYRQHYLAPERLRLPRG
                     TPVPAA"
     gene            complement(2562..>3266)
                     /locus_tag="VIN75_26560"
     CDS             complement(2562..>3266)
                     /locus_tag="VIN75_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEY8977805.1"
                     /translation="GLSQVLLRRGSEVVLDWDARRQARFTATDSQGRALAVALAEGSV
                     LRGGDVLVAADGSLIRVKAAEQPVLAVTPCPDHGTPLDLLRAAYHLGGRHVALALQAD
                     RLLLAPDAALAALLRAQHLIVTETKAAFDPENDAYEAPAHAHGHARHDHGHDHSHGHG
                     HDHGHGHAHHDHDHDHDHDHDHGHGHGHAHAKPKPAGKAVGIPVVGQAAPHVHGPHCG
                     HDHDHDHSHGHEKHGR"
BASE COUNT          374 a         1119 c         1248 g          525 t
ORIGIN      
        1 ccgacctgcc gatgccgccg ggcctgcgcg ccatcgcgcg tcggccctac gcgtggtgga
       61 cggatcgcct gctcgcgcgc cgggacagcc ccgtcatggc gcgcctgctg gtcgggctcg
      121 gcccggccac gcactggacg acgcgcatcc gcgacggcgt gctgttcgtg ctggtcggcg
      181 gcggcttgtg cgccgtcgtg tcggcgctgg cgggccgcga gttcggcgcc atggtcgcgc
      241 cgtggttgtc gttcagcctg ttgacgggcg cctgcacgcc ggcgctgcag gccgtggccc
      301 ggctgcaccg gacgcggagc gagcaggcgt tgctggtgct cttgccaggg gtgccgcgcg
      361 gcgcgggcct caaccgctgg ctggcgtggc gcatgagcct ggtcttcgtc gtcgccgcgc
      421 tgtgcggctt cgcgctggcc gcggcgctga atgcctttgc cgaagcgatc cagccgggcg
      481 tggtgaatcg gtcgaccggc ggcatggcct tcgcgttgtt cccggccttg ctgccgcagg
      541 tcgcgtggca atggcggcgg tgggcgcgcc ttcgcggcgt gacgggcctg gagatgtcga
      601 tgcccggcat cgcgccggtg ctgctgggcc tcgcggcgct gatgctgcac ctcgtgacgg
      661 gcacgggcta cctgccgatc ggtgtcgcct tcgcggccgt cgcgctggca tattgcgcgt
      721 ggcgctggcg ccgcatggcc gtcgagccga cggcgctgcc ggtgggccgg ctcgcctgag
      781 ggcggtcgcc ggggcgaccg ctcaggccgc cggcacgggc gtgccgcgcg gcaggcgcag
      841 gcgctcgggc gccaggtagt gctggcgata gatctcgaac ggcatcgtgt ccgcggcctc
      901 gatgcgctcc tgttcggcca gcgactcggt ggccagttcg gccatgtggg cggcgacgtc
      961 ggccggcagg tccagcgcga gcatgtgttc gcgcgtgcgc tgcgactgcg tgcgaacgaa
     1021 ggcgtcgtgc gagccgccgt gctgcgactg catctgcgcc agcacgcggg ccgacggcag
     1081 cgtctcgggt gcgagcaccg cggcgcgcgc ggccgccagc gcctgcacgt gggcgtcggt
     1141 gccttcgttg gcatcgagcc aggcggcgat gggcgcgcac tcggtgagga tctcgcgcag
     1201 ccagtccgtc atcagaacct cgacgccgcc gcgctcgagc ttcaggcccg gctcgcggcc
     1261 gcgcgacgcg acgcgctgct ggttgcgccc gagctcggcg atctcgttgg gcgagtcgag
     1321 cgggctgtcg tccagcaggc agtgcagcag gaagatgtcg atcatgcgca tcgtggccgg
     1381 ggcgatgccg gcgggcacga acgggtccag atccatgcag cgcacctcga tgtactcgac
     1441 gccgcgctcg cgcagcgcgt gcagcgggcg ctcgccgggg aagatcacgc gcttgggacg
     1501 gatggtgctg tagaactcgt tctcgatctg cagcaggctg tcgctcagct gcaggtacga
     1561 accgtcctcg gcctggatgc ccagcttctg gtacgcgggg tagggccgca cgagcgcgtc
     1621 cgacagcgag gccgcgtagc tgtcgaggct gttgtagctg acgcacagcg agttctgcgc
     1681 gtcgctctgg tagccgaggc ggcccatgcg cagcgacgtg gcgtgcggca tgtacagcgt
     1741 gtcggggccc atcggctgca ggtcgggcgc ctggtccgcg aagccgcgcg ggatcgcggg
     1801 cgacgcgccg aacaggtaca gcaacaggaa cgagcggcgg cggaagttgc ggatcagccc
     1861 gaagtactcc gccgtcgtca cttccggcag cgaccagttg tagtggatgc ccgagatcgt
     1921 ctgcatgcgg cggccgtagc gatggcccag gcccgtccga tagacggtct tggacatgcc
     1981 gacgttggac gtgccgtact ggccgagcgg gatccggtcc tcgtcgggcg gcaacgtaca
     2041 gggcatgctg ccgacccaca tcatctcgtc gccgagcgcg cggtagacgg cctggtgcac
     2101 ctgcgtcagc tcggcctcga ccgactccgc gctgcggtgc acgcccgtga tcagctccag
     2161 ctgcgattcg ctgaagtcgg tggtgatgtg cgggtgcgtc agggccgagc cgagcgcgcg
     2221 cgggtgcggc gtcatggcga gcgcgcccgc cggcgtcacg cgcaggcttt ccttctcgac
     2281 gcctcggccg atgccttgca ggcgagccgc gctcagtgcc ttcaggcgtt cttgtagacc
     2341 gcgcatgcgg tgcctcctgg gtgcttgtct ggcgcgaaag gtactggaaa tccgtgaccg
     2401 ttgcagccgt gggccgatga cgtccctgcc gtctttgtgc taggtcgagg gcgacgccgc
     2461 ccgctcgtca gtgcaaaccc tagcgcgacg gcggcggcgt cgggcccgcc ctggccaccc
     2521 ggcgcggcgc aggccgccgc cgcacgcccg gccgcggcgt gctagcggcc gtgcttctcg
     2581 tggccgtgcg agtggtcgtg gtcgtggtcg tgcccgcagt gcggcccgtg cacgtgcggg
     2641 gcggcctggc cgaccaccgg gatgcccacg gccttgccgg ccggcttcgg cttggcgtgc
     2701 gcgtggccgt ggccgtggcc gtgatcgtga tcgtgatcgt gatcgtgatc gtgatcgtga
     2761 tgcgcgtggc cgtgtccgtg atcgtgccca tgcccgtgcg aatggtcgtg gccatggtca
     2821 tgacgtgcat gcccgtgcgc atgcgcaggc gcctcgtacg cgtcgttctc gggatcgaac
     2881 gccgccttcg tctcggtgac gatcagatgc tgcgcgcgca gcaatgcggc cagggccgcg
     2941 tcgggcgcca gcagcaggcg gtctgcctgc agcgccagcg ccacgtggcg gccgcccagg
     3001 tggtaggcgg cgcgcagcag gtccagcggc gtgccgtggt cggggcaggg cgtcactgcc
     3061 agcaccggtt gctccgcggc cttcacgcgg atcagcgagc cgtcggccgc caccagcacg
     3121 tcgccgccgc gcagcacgct gccttcggcc agcgccacgg ccagcgcgcg gccctgcgaa
     3181 tcggtcgcgg tgaagcgggc ctggcgccgc gcgtcccagt ccagcaccac ttcgctgcca
     3241 cggcgcagca gcacctggct caggcc
//
LOCUS       DATCRO010000973         7967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_607382, whole genome shotgun sequence.
ACCESSION   DATCRO010000973 DATCRO010000000
VERSION     DATCRO010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607382"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VIN75_26565"
     CDS             <1..889
                     /locus_tag="VIN75_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY8977806.1"
                     /translation="FKVFSSAATMAGGRVVALRVKGGAEISRGEIDAYTEVVKIYGAK
                     GLAWIKVNDASKGREGLQSPIVKNLHDKAIAEILARTGATDGDLIFFGADKAKIVNDG
                     MGALRLKIGHSDFGKANGLFEDKWAPLWVVDFPMFERDDEENRWNAVHHPFTAPKDGH
                     EDLMDSDPGACIAKAYDMVLNGWELGGGSVRIHRAEVQSKVFSALNISKEDAQTKFGF
                     LLDALQYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRAQDLLTGAPSAVDEK
                     QLRELHIRLRNAVAATPAA"
     gene            complement(1054..1884)
                     /locus_tag="VIN75_26570"
     CDS             complement(1054..1884)
                     /locus_tag="VIN75_26570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977807.1"
                     /translation="MNPQVQAKVTALQAKIRQVDDYQSKGLLRAEEADAQRSALRKQL
                     MEAVIPNVPAPRLPMRARAWAVVAMLVLVGAVTAYLLSGHAGLRRRSETLLEQGKIAA
                     AQDARMRRERLERIRRGESLAPDKYGVFAPSSAVSAAASASAPLANAASKNASASADA
                     IAPLLSGRVTLASKFEGRVASDDTVFVTVRTPDDPTGLPLAQIRVEVSNLPFDFNVGA
                     REMIGSPDRFLHADKVIVSARISRSGSGRAKAGDLVGQSGPVAPWSNDVRFTIDQEVR
                     "
     gene            2134..4344
                     /locus_tag="VIN75_26575"
     CDS             2134..4344
                     /locus_tag="VIN75_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8977808.1"
                     /translation="MLHASFRLSALSAAILLLAAGQARAQAAAAPAAAASEPQTQQVV
                     ISADASAGGLKAPYAGGQVARGGRVGLLGSQDIMSTPLTITNYTAKLIQDQQASSLAD
                     VLQNDPAVRVTRGFGNFQQAYMVRGLVVYSDDISYNGLYGLLPRQYLAAQLVERVEVL
                     RGASAFLNGAAPGGSGLGGAVNVMPKRAGNAPQAEITVGLDAGQLSEAIDVGHRWGGH
                     KELGLRANVVHSEGDTAVKGERDKLDAALLGADWHLGGLRVSADVGTQDNDVKGATPN
                     IDVVGGVPVAPRADKQFAQKWTYSRERDTFGTLRAEVDILPSVTAWAAGGFRDGKEDN
                     QLEVPGIDAADASQTLYAAANARKDSIMTGEAGLRGSFATGPVRHTIVGSYGAYRAVS
                     RNAFAFSSFGGVAAGTLYDTTFIPVPDESYYTGGSDLAHPLTTLRVVTRSEALADTLA
                     LASDRVLLMLGARRQQIESTSYDYGTGALGTPASAGARITPVAGIVFKLNKQVSFFGT
                     YIEGLVAGDVAPRTWFDSNFVAHKVANMGEAFKPYQTRQDEIGIKVDTGGYGGTVSVF
                     RSRKPNYAVTPDAADPDASLFGLYSTQHNRGAEISVYGEAMPGVRVLGGASFLDARLL
                     GKEVIGSPRSQFNLGLDFDVPSVQGLALNVRGVHTARQFADTGNTEVVPAWTRLDVGA
                     RYEMDVGAHQVTLRAGIDNVTNRNYWASSGGYPGQGYLTVGAPRSVTVSGTFAY"
     gene            4548..5030
                     /gene="nudB"
                     /locus_tag="VIN75_26580"
     CDS             4548..5030
                     /gene="nudB"
                     /locus_tag="VIN75_26580"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEY8977809.1"
                     /translation="MSAASPESRPYKIPESVLVVIHTPALDVLLLERADAPGFWQSVT
                     GSKDRVEESLVQTCIREVGEETGIVVGGAGIPLAALRDCELVNVYEIYPRWRARYAPG
                     VTHNTEHVFALEVPAGTPVALAPREHTALRWLPWREAADACFSPSNAEAILHLPRWKE
                     "
     gene            5225..6034
                     /locus_tag="VIN75_26585"
     CDS             5225..6034
                     /locus_tag="VIN75_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8977810.1"
                     /translation="MNTASDFTILPSQTITAATRLRVATYNIHKGVRGLGAAKRLEIH
                     NLVLGVEALDADLVFLQEVRLHHTRDAARFDQTIMGWPEQGQAEFLAPEGYNSAYRTN
                     AFTRHGEHGNALLSRWPLGDVGHHDVSDHRFEQRGLLYVPVDWNGVTVHAIVAHFGLV
                     HSSRMRQAQRLAQFIASELPAGEPVLVAGDFNDWGERLDEPMAAAGLSRALSKDARLP
                     KRPTFPSRLPVFSLDRIYMRGFSCEGTMVPRGGGWARMSDHLPYVAELELA"
     gene            6140..7402
                     /gene="clsB"
                     /locus_tag="VIN75_26590"
     CDS             6140..7402
                     /gene="clsB"
                     /locus_tag="VIN75_26590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEY8977811.1"
                     /translation="MNTRAPWTLGVKPKFEGGHSIELLRGGDDLFPAMGTAIAQARHA
                     IWLATYIFHDDAVAQTLALLLAEAARRGVQVRVVVDGFGSKATLAALREWMVPAGVEL
                     AVFRPIDRWWSYLQPGQARRLHQKLCVVDSQVGFVGGINLIADRNDLHHGWSEAPRLD
                     FACRVRGAAVADIEQAVQATWTRAMLGRVWRDELAGLVRSAAPVARARRLATRLRIVR
                     PHRANPNIAQPPVSLAFVVRDNLRQRRSIERVYMEAIQHARHRIDLVTPYFYPDRHFR
                     KALAKAAERGVKVRLLLQGKIDYRIAGVAARVLYDELLARGVQIFEYTPAYLHAKMAV
                     VDDGWSTLGSSNIDPLSLLLNLEANVVVNDAPFARRVADELEIAMAASSPVMAPPYAK
                     GWLAVLRRTFVAMVAHWFLRMAGINGRY"
     gene            complement(7386..7862)
                     /locus_tag="VIN75_26595"
     CDS             complement(7386..7862)
                     /locus_tag="VIN75_26595"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY8977812.1"
                     /translation="MEQPPSPANAPPPRSERPTLDELTAIALGADPRRMSPVQLYLGV
                     KGRIPRSTYWLHGVLSLLLAGVVLDGLLAIARVEPDTANGIVSLLLLWPLIAVSAKRQ
                     HDFDFSAWWALIHFVPAVGSLVLLLADGLVPGTRGPNRFGPDPRQAGAAKRLNSGH"
BASE COUNT         1171 a         2831 c         2849 g         1116 t
ORIGIN      
        1 cttcaaggtg ttctcgtcgg ccgcgacgat ggccggcggc cgcgtcgtgg cgctgcgcgt
       61 caagggcggc gccgagatca gccgcggcga gatcgacgcc tacaccgagg tcgtcaagat
      121 ctacggcgcc aagggcctgg cgtggatcaa ggtcaacgac gcgtccaagg gccgcgaagg
      181 cctgcagtcg cccatcgtca agaacctgca cgacaaggcg atcgccgaga tcctggcgcg
      241 caccggcgcg accgacggcg acctgatctt cttcggcgcc gacaaggcca agatcgtcaa
      301 cgacggcatg ggtgcgctgc gcctgaagat cggccactcc gacttcggca aggccaacgg
      361 cctgttcgag gacaagtggg cgccgctgtg ggtcgtcgac ttcccgatgt tcgagcgcga
      421 cgacgaggag aaccgctgga acgcggtgca ccacccgttc acggcgccca aggatggcca
      481 cgaggacctg atggattcgg atccgggcgc ctgcatcgcc aaggcctacg acatggtcct
      541 gaacggctgg gaactgggcg gcggctcggt gcgtatccac cgcgccgagg tgcagagcaa
      601 ggtgttctcc gcgctgaaca tcagcaagga agacgcgcag accaagttcg gcttccttct
      661 ggacgcgctg cagtacggcg ccccgccgca cggcggcctg gccttcggcc tggatcgcat
      721 cgtcacgatg atgaccggcg ccgagtcgat ccgcgacgtg atcgccttcc ccaagaccca
      781 gcgcgcgcag gacctgctca ccggcgcccc gagcgcggtc gacgagaagc agctgcgcga
      841 gctgcacatc cgcctgcgca acgcggtggc cgccacgccg gccgcctgag ccgcacgctg
      901 acctgaccgg cgccgcgcgc gccgggtcga ggcccgcatc gtcctgccat tcgcgggcgg
      961 cgcgggcctc ctgcattggg cggcaggagg gacgcgccag cgagcggggg cgtcgacccg
     1021 tgcctcgatc cggcgtcaga ggccatgcgc cgctcagcgc acttcctggt cgatcgtgaa
     1081 gcgcacgtcg ttggaccacg gcgcgaccgg ccccgactgg cccaccaggt cgcccgcctt
     1141 cgcgcggccg ctgccgctcc tggagatgcg cgccgacacg atcaccttgt cggcatgcag
     1201 gaagcggtcg ggcgagccga tcatctcgcg cgcgccgacg ttgaagtcga acggcaggtt
     1261 gctgacctcg acgcggatct gcgccagcgg caggccggtc gggtcgtcgg gcgtgcgcac
     1321 ggtgacgaag acggtgtcgt ccgaggcgac gcggccctcg aacttcgaag cgagcgtgac
     1381 gcggcccgac agcagcgggg cgatggcatc cgccgaggcg ctggcgttct tcgatgcggc
     1441 gttcgcgagc ggggccgagg ccgacgcggc ggccgacacg gcggacgagg gcgcgaagac
     1501 gccgtacttg tccggtgcca gcgactcgcc gcgcctgatg cgctccagcc tctcgcgccg
     1561 catccgggcg tcctgcgcgg cggcgatctt gccctgctcc agcagggtct cgctgcggcg
     1621 gcgcaggccg gcatggcccg acagcaggta ggccgtgacg gcgcccacca gcaccagcat
     1681 cgcgacgacc gcccaggcgc gcgcacgcat cggcaacctg ggcgcgggca cgttcgggat
     1741 gacggcttcc atcagctgct tgcgcaacgc gctgcgctgg gcgtcggcct cctcggcgcg
     1801 gagcaggccc ttgctctggt aatcgtcgac ctggcggatc ttggcctgca gcgcggtgac
     1861 cttggcttgg acttgcggat tcatgggcat ggcgaggtga atcggccatt ttcgcaggtc
     1921 gcggcgcgct gccggccgcg agtcgcggga cggtcgctcg gcccgcttcg ccgaccggtc
     1981 cagctcccgg gccagggcgg cctaccatgg cccgcgacga tcgatgttcg cgtggcacgc
     2041 gcccggaccg tcgacaccgc cgagccaggg agaattgcag ctcatatgaa tgagaataag
     2101 tcgcgtctgc gggtcatgca cctcaggaaa tccatgctgc atgccagctt ccgcctcagc
     2161 gcgttgtccg ccgccatcct cctgctggcc gcgggccagg cccgcgcgca ggccgccgcc
     2221 gcgccggccg ccgccgcgag cgagccgcag acgcagcagg tcgtcatcag cgccgacgca
     2281 tcggcgggcg gcctcaaggc gccgtacgcg ggcggccagg tggcccgcgg cggccgcgtc
     2341 ggcctgctgg gcagccagga catcatgtcc acgccgctga ccatcaccaa ctacaccgcc
     2401 aagctgatcc aggaccagca ggcctcgagc ctcgccgacg tgctccagaa cgacccggcc
     2461 gtgcgcgtca cgcgcggctt cggcaacttc cagcaggcct acatggtgcg tggcctggtc
     2521 gtctattccg atgacatctc ctacaacggc ctgtacggcc tgctgccgcg ccagtacctg
     2581 gcggcgcaac tggtcgagcg ggtcgaggtg ctgcgcggcg cgagcgcctt cctcaacggc
     2641 gcggccccgg gcggcagcgg cctgggcggc gcggtcaacg tcatgcccaa gcgagcgggc
     2701 aatgcgccgc aggccgagat caccgtgggc ctggatgcgg gccagctgtc ggaggccatc
     2761 gacgtcggcc atcgctgggg cgggcataag gagctgggcc tgcgcgccaa cgtcgtccac
     2821 agcgagggcg acaccgcggt caagggcgag cgcgacaagc tcgacgcggc gctgctgggc
     2881 gccgactggc acctgggcgg cctgcgcgtg tcggccgacg tcggcacgca ggacaacgac
     2941 gtcaagggcg cgacacccaa catcgacgtg gtcggcggcg tgcccgtcgc gccgcgcgcg
     3001 gacaagcagt tcgcgcagaa gtggacgtac tcgcgcgagc gcgacacctt cggcacgctg
     3061 cgggccgaag tcgacatcct gccctcggtg acggcctggg ccgccggcgg tttccgcgac
     3121 ggcaaggagg acaaccagct cgaggtgccc ggcatcgacg cggccgacgc ctcgcagacg
     3181 ctgtacgcgg cggccaacgc ccgcaaggac tcgatcatga cgggcgaggc gggcctgcgc
     3241 ggcagcttcg ccaccggccc cgtcaggcac acgatcgtcg gttcgtacgg cgcctaccgg
     3301 gcagtgtcca ggaacgcgtt cgcgttctcc agcttcggcg gcgtggcggc cggcacgttg
     3361 tacgacacga ccttcatccc cgtgcccgac gagtcgtact acaccggcgg cagcgacctc
     3421 gcgcacccgc tgacgacgct gcgcgtggtc acccgcagcg aggcgctggc cgacacgctg
     3481 gccttggcga gcgaccgcgt gctgctgatg ctcggcgcgc gccgccagca gatcgagagc
     3541 acgtcgtacg actacggcac cggcgcgctc ggcacgcccg ccagcgccgg ggcgcgcatc
     3601 acgccggtgg ccggcatcgt gttcaagctg aacaagcagg tgtcgttctt cggcacgtac
     3661 atcgaaggac tggtggcggg cgacgtcgcg ccccggacct ggttcgacag caacttcgtc
     3721 gcgcacaagg tcgccaacat gggcgaggca ttcaagccgt accagaccag gcaggacgag
     3781 atcggcatca aggtcgacac cggcggctac ggcggcacgg tgagcgtgtt caggtcgcgc
     3841 aagccgaact acgccgtcac gcccgacgcc gccgaccccg atgcgagcct gttcggcctg
     3901 tacagcaccc agcacaatcg cggcgccgag atctcggtgt acggcgaggc catgccgggc
     3961 gtgcgcgtgc tgggcggcgc gagcttcctc gatgccaggc tcctgggcaa ggaggtgatc
     4021 ggctcgccgc gctcgcagtt caacctgggc ctggacttcg acgtgcccag cgtgcagggc
     4081 cttgcgctga acgtgcgcgg cgtgcacacg gccaggcagt tcgccgacac cggcaacacc
     4141 gaggtggtgc cggcctggac gcgcctggat gtcggcgcgc gctacgagat ggacgtcggc
     4201 gcgcaccagg tcacgctgcg cgcgggcatc gacaacgtca ccaaccgcaa ctactgggcc
     4261 tcgtcgggcg gctatccggg ccagggctac ctgaccgtgg gcgcgccgcg cagcgtgacg
     4321 gtctcgggca ccttcgcgta ctgatgtcgc ccggcacgac gcgcgtccgg tcctggctgc
     4381 gcaagtggag cagccgggtc tcgacggact tcctgctcgt cctgtgcccg acgcgacgac
     4441 cggcctgcgg ccaatgggac tggatgacgt ggatcggtct gggcgtgccg tgcgtggcca
     4501 gcgtctggtt cgcccttcac gggcgggacg cgcgctagca tgcgcgcatg agtgccgcgt
     4561 cccccgagtc ccgtccgtac aagatccccg agtccgtcct cgtcgtcatc catacgccgg
     4621 cgctcgacgt gctgctgctg gagcgcgccg acgcgcccgg gttctggcag agcgtcaccg
     4681 gctccaagga tcgcgtcgag gaatcgctgg tccagacctg catccgcgag gtcggcgaag
     4741 agaccggcat cgtcgtcggc ggggcgggca tcccgctggc cgcgctgcgc gactgcgagc
     4801 tcgtcaacgt ctacgagatc tacccgcgct ggcgtgcccg ctacgcgccc ggggtgacgc
     4861 acaacaccga gcacgtcttc gcgctggagg tgcccgcggg cacgccggtc gccctggcgc
     4921 cgcgcgagca cacggcactg cgctggctgc cgtggcgcga ggcggcggac gcgtgtttct
     4981 cgccgtcgaa cgccgaggcg atattgcacc tgccgcgttg gaaggaatga cgcgccgcgc
     5041 cggacggcgc cgcgtcatcc ccgtgatgcc gtcgtcctga cggcgcgagg gtcatcgaat
     5101 cccgcgcccg acagtggcat catccaaggg catgaggatt gccagactga tcgccccgct
     5161 gaaaggtccg cggaggaact cgtcgacttc attgcacgcc ttgcggcgtg gacttcctgc
     5221 cgtgatgaac accgcgtcgg acttcacgat cctgccctcc cagacgatca ccgccgcgac
     5281 gcggcttcgg gtcgccacgt acaacatcca caagggcgtg cgcgggctcg gcgcggccaa
     5341 gcggctggag atccacaacc tggtgctggg cgtcgaggcg ctggacgccg acctcgtctt
     5401 cctgcaggag gtgcggctgc accacacgcg cgacgccgcg cgcttcgacc agaccatcat
     5461 gggctggccc gagcagggcc aggccgaatt cctggcgccc gagggctaca actcggccta
     5521 ccgcaccaac gcgttcacca ggcacggcga gcacggcaac gcgctgctct cgcgctggcc
     5581 gctcggcgac gtggggcacc atgacgtgtc cgaccaccgc ttcgagcagc gcggcctgct
     5641 gtacgtgccg gtcgactgga acggcgtgac ggtgcatgcg atcgtggcgc acttcggcct
     5701 cgtgcattcc agccgcatgc gccaggcgca gcggctcgcc cagttcatcg ccagcgagct
     5761 gccggccggc gaaccggtgc tggtggccgg ggacttcaac gactggggcg aacgcctgga
     5821 cgagccgatg gccgccgccg gcctgtcgcg cgcgctgtcc aaggacgcgc gcctgcccaa
     5881 gcggccgacg ttcccgtcgc gcctgccggt gttctcgctc gatcgcatct acatgcgcgg
     5941 cttcagctgc gagggcacga tggtgccgcg cggcggcggc tgggcgcgca tgtccgacca
     6001 cctgccgtac gtcgccgagc tcgagctggc ttgagctaaa ttgacggctg ggcgcgcggc
     6061 gcccgtcgtg gccgggtcct tccggccgac cggaaccaac cgtgggagtc ttgcgattcg
     6121 cgccaagcgt cgtgagcgcg tgaacacgcg cgccccctgg accctcggcg tcaagccgaa
     6181 gttcgagggc ggccattcga tcgagctgct gcgcggcggc gacgacctgt tccccgcgat
     6241 gggcacggcg atcgcgcagg ctcgccacgc gatctggctg gccacgtaca tcttccatga
     6301 cgacgccgtc gcccagacgc tggcgctgct gctggccgag gcggcgcgcc gcggcgtgca
     6361 ggtgcgcgtg gtggtggacg gcttcggctc caaggcgacg ctggcggcgc tgcgcgaatg
     6421 gatggtgccc gcgggcgtgg agctggcggt gttccggccg atcgaccgct ggtggagcta
     6481 cctgcagccg ggccaggcgc ggcggctgca ccagaagctg tgcgtcgtcg acagccaggt
     6541 cggcttcgtc ggcggcatca acctgatcgc cgaccgcaac gacctgcacc acggctggag
     6601 cgaggccccg cgcctggact tcgcgtgccg cgtgcgcggc gccgccgtgg cggacatcga
     6661 gcaggcggtg caggcgacct ggacccgcgc gatgctgggg cgcgtgtggc gcgacgagct
     6721 cgcgggcctg gtgcgcagcg cggcaccggt ggcgcgcgcc aggcgcctcg cgacccgcct
     6781 gcgcatcgtg cggccgcatc gcgccaatcc caacatcgcg cagccgccgg tgagcctcgc
     6841 gttcgtggtg cgcgacaacc tgcgccagcg ccgttcgatc gaacgcgtgt acatggaggc
     6901 catccagcac gcgcgtcatc gcatcgacct cgtcacgccg tacttctacc ccgaccgcca
     6961 cttccgcaag gcgctggcca aggccgccga gcgcggcgtg aaggtgcggc tgctgctgca
     7021 gggcaagatc gactaccgca tcgccggcgt cgccgcgcgg gtgctgtacg acgaactgct
     7081 ggcccgcggc gtgcagatct tcgagtacac gccggcctac ctgcacgcga agatggccgt
     7141 ggtcgacgac ggctggtcca cgctcggcag ctccaacatc gacccgctgt cgctgctgct
     7201 gaacctggag gccaacgtcg tcgtcaacga cgcgccgttc gcgcgccgcg tggccgacga
     7261 gctggagatc gcgatggccg cgtccagccc cgtgatggcg ccgccctatg ccaagggctg
     7321 gctggccgtg ctgcgccgca ccttcgtggc gatggtggcg cactggttcc tgcgcatggc
     7381 gggcatcaat ggccgctatt gaggcgcttg gccgccccgg cttggcgcgg gtcgggcccg
     7441 aagcggttgg ggccgcgcgt gccgggcacc aggccgtcgg ccagcagcag cacgagcgag
     7501 cccacggccg gcacgaagtg gatcagcgcc caccaggccg agaagtcgaa gtcgtgctgg
     7561 cgcttggccg acaccgcgat cagcggccac agcagcagca ggctcacgat cccgttggcc
     7621 gtgtccggct cgacgcgcgc gatggccagc aggccgtcga gcaccacgcc ggccagcagc
     7681 agcgacagca cgccgtgcag ccaataggtg ctgcgcggga tgcggccctt cacgcccagg
     7741 tacagctgca ccgggctcat gcgccgcggg tcggcgccga gcgcgatggc cgtgagctcg
     7801 tccagcgtcg ggcgctcgga gcggggcggc ggcgcgttcg ccggggaagg gggctgttcc
     7861 atccgggaca gtattccatg cggcccgccg tgcgatgtga ccgcgggtat cgggtcgtga
     7921 cgaacgcggc ggcaccgttg cgggcgcgcc gcagtcgggc ggtcgcg
//
LOCUS       DATCRO010000974         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_371142, whole genome shotgun sequence.
ACCESSION   DATCRO010000974 DATCRO010000000
VERSION     DATCRO010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371142"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..977
                     /gene="secF"
                     /locus_tag="VIN75_26600"
     CDS             3..977
                     /gene="secF"
                     /locus_tag="VIN75_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8977813.1"
                     /translation="MRHALVFNIISALTFLAAVFFLVTRGLNFSIEFTGGTAVEATYA
                     QSVNLERLRGVVGGLGYGDIQVQNYGTSHDVIIRVPTKAGIDQKKVPDRVFGALCAAE
                     SGSISAADPTRPTCLNAAKAEPVVMKRSELVGPSVGDELMRNGIYAMISVVIGIMLYL
                     AFRFEWKFSIAAIVANLHDVVIILGFFAFFQWEFSLSVLAGVLAVLGYSVNESVVIFD
                     RIREAFRVFRKLSTMQVIDHAITSTISRTIITHFCTQMMVLSMLIFGGPTLHYFAMAL
                     TIGICFGIYSSVFVAAAIAMWLGVKREDLVRAAKAEIDPNDPNAGAVV"
     gene            1035..3845
                     /locus_tag="VIN75_26605"
     CDS             1035..3845
                     /locus_tag="VIN75_26605"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY8977814.1"
                     /translation="MAEGKSSPEIARRARRMFAEQLAEAGSRLLAAVLEAARTALDKP
                     AERSVAMQRRDTMQALMAHGEEWIERQNEMLRDAATADRPNTTTGGLVSAMTGHSQQL
                     MLVDDETVQKEIFISRLAQAIVDQAVWELNDLKTRVAALEGLEELAEHDLFRPQQVAK
                     VIVRSFIDSDFGTGAWADVENIMKVELAALCTEAYHDANRFLVEKGVLPEVNLRTLIR
                     RSVDRGGSMVASRQMPLEDFKSTSGGALTTGSGGTQFAPTQYGGAGGHGAGPGGYADS
                     QRGGHTSYGGVGGGHGGQGGPGGQGGYGGGYVGGQAGHGGGPAGQNGPVGYAGGQAGQ
                     GGQGDRGGQGAPSNGPGAGHAGATGGAGSAGYGVQPAAAGQAGGPGGPGQAGGGYNAT
                     SYGATGYGATGYGSTGYGRGANPYGPDSQPGTLNGAPNVGVSNKRGPGGQFLSPQQVQ
                     VDATLRRFGRTMERHVQGFMNTQRFVMPSNEMTGAIAVAQRAVVEQVEARHVEGAVMV
                     PAEVAGVLQEQKQTLKTQASTQEERATIEVVAMMFAAILTEERIPSAVRVWFARLQMP
                     TLRVAMAEPDFFSSAQHPARRLIDRMGACVMGFDAAPQGAGPELESEVARVVQVVEAF
                     PETGRKVFQTVLVEFERFLEKFFRDQNETTKKGVSLASQVEQRETLAIQYTIELRKLL
                     DGVPVQDGVREFLFQIWADILATTAMRHGLQGEETRAVRETALDLVWIASAKTTREER
                     AEVIRRLGPMLAALRQGMTSSGLPHDRQEASLRSLNMALTAAFAARTSTIDPDQFGRM
                     KQRLEALDEILPDADFELDDSFALDLSGHESDELEIVAEGGATPTAGALAATDEMLVG
                     AWYMLEYRDRQEAMQLAWQGMRKQLSLFVSAGGRCVLFQRRRLAAFLQAQLLVPAQDE
                     SLTIAATRTAIEKINQDPDRLK"
     gene            complement(4037..4489)
                     /locus_tag="VIN75_26610"
     CDS             complement(4037..4489)
                     /locus_tag="VIN75_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY8977815.1"
                     /translation="MFDVLVYLYENYWRPDACPDHDQLSRKLTAVGFETDEIQEALHW
                     LDGLADTSQGYTGTQSEDAIRLYSDREIESLGETSIGFVTFLESAGVLPPPMREMVID
                     RAMAAGGNPLDLDDMKIIILMVFWSLGEEPDALILDELFVAPEERLIH"
     gene            complement(4681..>5694)
                     /gene="dprA"
                     /locus_tag="VIN75_26615"
     CDS             complement(4681..>5694)
                     /gene="dprA"
                     /locus_tag="VIN75_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8977816.1"
                     /translation="DTVQALRGDAARHARQVDATRRWLDGATAQVARDIVVLGDPRYP
                     PLLLHTADPPLLLFTLGRVELLAADSVAIVGSRRATPQGLDNARAFARFLSERRLTVV
                     SGLAMGIDGAAHEGALAGPGSTVAFVGTGLDRVYPARHVPLMKRIAEQGLVASEYPLG
                     APARPENFPRRNRLIAGISRGTLVVEAALESGSLITARMAVECGREVFAIPGSIHSPQ
                     ATGCHRLIQEGAKLVESGDDVLRELRPDDSPRRARLSPPQPSLFAAPGAGPRDANEAP
                     ETPGTDAALRTALGYDPVSFDVLSARTGLPADQLAAQLLELELAGMVQRLPGGLLQRR
                     GQA"
BASE COUNT          873 a         1960 c         2046 g          815 t
ORIGIN      
        1 tcatgcggca cgcgctggtg ttcaacatca tctcggcgct cacgttcctc gcggccgtgt
       61 tcttcctggt cacccgcggc ctgaacttct cgatcgagtt caccggcggc acggccgtcg
      121 aggccacgta cgcgcagagc gtcaacctgg aacgcctgcg cggcgtcgtc ggcggcctgg
      181 gctacggcga catccaggtg cagaactacg gcacctcgca cgacgtgatc atccgcgtgc
      241 caaccaaggc cggcatcgac cagaagaagg tgcctgaccg cgtcttcggc gcgctgtgcg
      301 ccgccgagtc cggcagcatc agcgccgccg acccgacccg gccgacctgc ctgaacgcgg
      361 ccaaggccga gccggtggtg atgaagcgca gcgagctggt ggggccgtcc gtcggcgacg
      421 agctgatgcg caacggcatc tacgcgatga tcagcgtggt catcggcatc atgctgtacc
      481 tggcgttccg cttcgaatgg aagttctcca tcgccgcgat cgtcgccaac ctgcacgacg
      541 tcgtcatcat cctgggcttc ttcgcgttct tccagtggga gttcagcctg tcggtgctgg
      601 cgggcgtgct tgcggtgctg ggctactccg tcaacgagtc cgtggtcatc ttcgaccgga
      661 tccgcgaggc cttccgcgtc ttccgcaagc tgtcgacgat gcaggtgatc gaccacgcga
      721 tcaccagcac catcagccgc acgatcatca cccacttctg cacccagatg atggtgctgt
      781 cgatgctgat cttcggcggc ccgacgctgc actatttcgc gatggcgctg accatcggca
      841 tctgcttcgg catctattcg tcggtgttcg tggcggccgc catcgccatg tggctgggcg
      901 tcaagcggga agacctggtc cgcgccgcga aggccgagat cgacccgaat gacccgaacg
      961 cgggcgccgt ggtgtagatt gcgcacatag cgcagaccgc agcgctcact ttgtaaggga
     1021 agcgaagcag ccggatggcc gaaggcaaat cgtcgccaga gatcgcgcga agagcgcggc
     1081 ggatgttcgc cgagcaactg gccgaggccg gctctcgcct gctggcggcc gttctggagg
     1141 ccgcccgcac ggcgctggac aagccggccg agcgctccgt ggcgatgcag cgccgcgaca
     1201 ccatgcaggc gctgatggcg cacggcgagg agtggatcga gcggcagaac gagatgctgc
     1261 gcgacgccgc caccgccgat cgccccaaca ccacgaccgg cggcctggtc tcggccatga
     1321 ccggccactc ccagcaactg atgctggtcg acgacgagac ggtgcagaag gagatcttca
     1381 tctcccggct ggcgcaggcc atcgtcgacc aggccgtctg ggagctgaac gacctcaaga
     1441 cgcgcgtcgc cgcgctcgag ggactggagg aattggccga gcacgacctg ttccgcccgc
     1501 agcaggtggc caaggtcatc gtgcgctcgt tcatcgacag cgatttcggc accggtgcct
     1561 gggccgacgt cgagaacatc atgaaggtgg agctcgcggc cctgtgcacc gaggcctacc
     1621 acgacgcgaa ccgcttcctc gtggagaagg gcgtgctgcc cgaggtcaac ctgcgcacgc
     1681 tgatccggcg cagcgtcgac cgcggcggct cgatggtggc ctcgcgccag atgccgctgg
     1741 aagacttcaa gtcgaccagc ggcggcgcgc tgaccaccgg cagcggcggc acccagttcg
     1801 cgccgaccca gtacggcggc gcgggcggcc acggcgcggg ccccggcggc tacgccgaca
     1861 gccagcgcgg cggccatacg agctacggcg gcgtgggcgg cggccatgga ggccagggcg
     1921 gtcctggcgg gcagggcggc tatggcggcg gctacgtcgg cggccaggcc ggccacggcg
     1981 gtgggcccgc cggccagaac ggcccggtgg gctacgcggg cggccaagcc gggcagggcg
     2041 gccaaggtga ccgcggcggc caaggcgcac cgtcgaatgg cccgggggcg ggccacgcgg
     2101 gcgcgactgg cggggcgggc agcgccggct acggcgtcca gccggccgcg gccggccaag
     2161 ccggcggccc gggtggcccg ggccaggcgg ggggcggcta caacgcgacg agctacggcg
     2221 cgaccggcta cggggccacg ggctatggtt cgaccggcta cggccgcggc gcgaatccct
     2281 acggcccgga cagccagccg ggcacgctga acggcgcgcc caacgtgggc gtcagcaaca
     2341 agcgcgggcc cggcggccag ttcctgtcgc cgcagcaggt gcaggtcgac gccacgctgc
     2401 gccgcttcgg ccgcacgatg gagcgccacg tccagggctt catgaacacg cagcggttcg
     2461 tgatgccgtc caacgagatg acgggcgcga tcgcggtcgc gcagcgcgcc gtcgtcgaac
     2521 aggtcgaggc gcggcacgtc gagggcgccg tcatggtgcc ggccgaggtc gccggcgtcc
     2581 tgcaggaaca gaagcagacc ctcaagacgc aggcgagcac gcaggaggag cgcgccacga
     2641 tcgaggtcgt cgcgatgatg ttcgcggcca tcctcaccga ggagcgcatc ccgtcggcgg
     2701 tgcgcgtgtg gttcgcgcgc ctgcagatgc ccacgctgcg cgtcgcgatg gccgagcccg
     2761 acttcttctc gtcggcgcag catccggcgc gccgcctgat cgaccgcatg ggcgcctgcg
     2821 tgatgggctt cgacgccgcg ccgcagggcg ccggccccga gctcgagagc gaggtggcgc
     2881 gggtcgtcca ggtcgtcgag gcgttcccgg agacgggccg caaggtgttc cagacggtgc
     2941 tcgtcgagtt cgagcgcttc ctcgagaagt tcttccgcga ccagaacgag acgaccaaga
     3001 agggcgtctc gctcgcgagc caggtcgagc agcgcgagac gctggccatc cagtacacca
     3061 tcgagctgcg caagctgctg gacggcgtgc cggtgcagga cggcgtgcgc gagttcctgt
     3121 tccagatctg ggccgacatc ctggccacca cagcgatgcg ccacggcctg cagggcgagg
     3181 agacgcgcgc cgtgcgcgag accgcgctgg acctggtatg gatcgcgagc gccaagacca
     3241 cgcgcgagga gcgcgccgag gtcatccgcc ggctcgggcc gatgctggcc gccctgcgcc
     3301 agggcatgac gtccagcggc ctgccgcacg atcgccagga ggcctcgctg cgctcgctca
     3361 acatggcgct gacggcggcc ttcgcggccc gcacgtccac catcgatccc gaccagttcg
     3421 gtcgcatgaa gcagcgtctg gaggcgctcg acgagatcct gccggacgct gacttcgagc
     3481 tggacgactc gttcgcgctc gacctgtccg gccacgagtc cgacgagctc gagatcgtcg
     3541 ccgaaggcgg cgccacgccg accgccggcg cgctcgcggc caccgacgag atgctggtgg
     3601 gcgcgtggta catgctggag taccgcgacc gccaggaggc gatgcagctg gcatggcaag
     3661 gcatgcgcaa gcagctgtcg ctgttcgtgt cggccggcgg ccgctgcgtg ctgttccagc
     3721 gccggcgcct ggcggcgttt ctgcaggcgc aattgctggt gcccgcgcag gacgagtcgc
     3781 tcacgatcgc cgccacgcgc accgcgatcg agaagatcaa ccaggatccg gaccgcctca
     3841 agtagggaac agggtcagag ggaacggggt caggtcttga aggttgcgcg ccaaatcgct
     3901 tgcatcaggg gagcggtgcc cgcgcaactt tcaagacctg accccgtctt cctgaccccg
     3961 tcttcccaac cccgccttcc accaacgaca acggctcccg cgggagccgt ttctcatgac
     4021 gtgctcgacg ccgccgtcag tggatcaggc gctcttccgg cgcgacgaac agctcgtcca
     4081 ggatcagcgc gtcgggctcc tcgcccaggc tccagaacac catcaggatg atgatcttca
     4141 tgtcgtcgag gtcgagcggg ttgccgccgg cggccatggc acggtcgatc accatctcgc
     4201 gcatcggcgg cggcaggacg ccggccgact cgaggaaggt gacgaagccg atcgaggtct
     4261 cgcccagcga ttcgatctcg cggtcggaat acaggcggat ggcgtcctcg gactgcgtgc
     4321 ccgtgtagcc ttgcgacgtg tcggccaggc cgtcgagcca gtgcagggct tcctggatct
     4381 cgtccgtctc gaacccgacc gcagtgagct tgcgcgacag ctggtcgtgg tctggacagg
     4441 cgtccggtcg ccagtagttc tcgtacaggt agacaaggac gtcgaacata tggactgact
     4501 ataccgcagc gaccggtggc ggcaagcgcg agttgggcgg cgcaagcccc gctgttggac
     4561 ctttgtgaaa tcgtcgttgc gggggccgga tttacatcgt ccggatgcgc cgctggccct
     4621 ttttggtgcg acgatcgcag gctcgaccga cgggatgccg gccggattcc ctccccgccc
     4681 tcaggcctgt ccgcgccgct gcagcagacc accgggcagc cgctgcacca tccctgccag
     4741 ctcgagttcg agcaactgcg ccgcgagctg gtcggccggc aggccggtgc gggccgacag
     4801 cacgtcgaag ctgaccgggt cgtagccgag cgccgtgcgc agggcggcgt cggtgccggg
     4861 ggtctcgggc gcctcgttcg cgtcgcgcgg gccggcgccc ggggcggcga acagcgaggg
     4921 ctgcggcggc gacagccgcg cgcgccgcgg gctgtcgtcc ggccgcagct cgcgcagcac
     4981 gtcgtcgccg ctctccacga gcttggcgcc ttcctggatc agccgatggc agcccgtggc
     5041 ctgcggcgag tggatcgagc cggggatcgc gaagacctcg cgtccgcatt ccacggccat
     5101 gcgcgccgtg atcaacgacc ccgattccag cgccgcctcc accaccagcg tgccgcgcga
     5161 gatgccggcg atcaggcggt tgcggcgcgg aaagttctct ggccgggctg gcgcccctag
     5221 cgggtactca ctcgcgacca ggccctgttc ggcgatgcgc ttcatcagcg ggacgtggcg
     5281 ggcggggtag acacgatcca ggccggtgcc gacgaaggcg acggtgctgc ccgggcctgc
     5341 gagcgcgcct tcgtgcgcgg cgccgtcgat gcccatcgcc aggcccgaga cgacggtgag
     5401 ccggcgctcg ctcaggaagc gcgcgaacgc gcgtgcgttg tccaggccct gcggcgtggc
     5461 gcggcggctg cccacgatgg ccacgctgtc ggccgccaac agctcgacgc ggccgagcgt
     5521 gaacagcagc agcggcggat cggccgtgtg cagcagcagg ggcggatagc gcgggtcgcc
     5581 gaggacgacg atgtcgcggg cgacttgcgc cgtggcgccg tccagccagc gccgggtggc
     5641 gtcgacctgg cgggcgtggc gggccgcgtc gccgcgcagg gcttgcaccg tgtc
//
LOCUS       DATCRO010000975        13793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_359607, whole genome shotgun sequence.
ACCESSION   DATCRO010000975 DATCRO010000000
VERSION     DATCRO010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359607"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIN75_26620"
     CDS             <1..261
                     /locus_tag="VIN75_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8977817.1"
                     /translation="DPAGTAEPAGLRFRAAGMAANRKRLGLSAADFGLLVGATGQSIY
                     AWEAGKAKPRPQALAAIAALRGVGKREVEARLAGLKGGTAPD"
     gene            complement(347..742)
                     /locus_tag="VIN75_26625"
     CDS             complement(347..742)
                     /locus_tag="VIN75_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY8977818.1"
                     /translation="MAIPKAGTNVRGSRTGRPIMVVLDLLGRRGALRVLWELRGEPLT
                     FRALQEACDTNPSLLNTRLTELRASNLVDHSEGGYHLTTSGRSLCVAMQPLSAWASAW
                     AAAEERRVGKPAQPRRDSTRKSSKSRAVE"
     gene            791..1453
                     /locus_tag="VIN75_26630"
     CDS             791..1453
                     /locus_tag="VIN75_26630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8977819.1"
                     /translation="MKVHMAARISPLDPPYDAKLQCRLDAVMHGAPPLRLFTTLARDS
                     RLFDKFFSGSLLDRGHLTLRQREIVIDRTAALCGSEYEWGVHVALFAPKARLDAAAIH
                     SLAHGSADDPCWSDEDRDLLRLCDALHRDCDVGDELWRALQMRFSEGALLELLMLAGF
                     YRTVSYLTNVLRLPLEEGVARFPGPGLPPAGAQRFGCASTRPSHGSGDEPGDDLPPGS
                     SG"
     gene            1526..1852
                     /locus_tag="VIN75_26635"
     CDS             1526..1852
                     /locus_tag="VIN75_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY8977820.1"
                     /translation="MIKVNVMYPYTEGARFDHAYYRDRHMPMMKARLGSACAYYTVEK
                     GLAGRAPGAPPAFVAMCAFICDSAEGYEAALQEHRAEIMADIANYTDIAPVLQFSEVV
                     VERSDR"
     gene            1902..2762
                     /gene="nadC"
                     /locus_tag="VIN75_26640"
     CDS             1902..2762
                     /gene="nadC"
                     /locus_tag="VIN75_26640"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY8977821.1"
                     /translation="MTFTLAGFDLAAFVNATLAEDLGIGLPGGGRDITSESVIPADAR
                     FAGVMDSREAITVAGLPLAAAFFRHLDPACAIELLVQDGQSVTPGTALMRIAGNARAL
                     LTAERNALNTVQHLSGIATMTRAYVEAMHGRATLLDTRKTIPGLRALEKYAARMGGAT
                     NYRMGLWDAVMIKDNHVAVAGGVGPAVARAKAAGVERIICEVDRIDQIEPAIMAGATH
                     ILLDNMTPTMLSDAVALVAGRAATEASGGVRLDNIGAVAASGVDYVSVGRLMQSAPAA
                     DIGLDFELVR"
     gene            complement(3166..3954)
                     /locus_tag="VIN75_26645"
     CDS             complement(3166..3954)
                     /locus_tag="VIN75_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8977822.1"
                     /translation="MRRWENWERPHDARARRPEGPDHVGHRRPGDASLRLGVEDVMTT
                     GRQAVTTQQDEVYGRLRQWVTVGRFVPGERLKIRQVAAEMGVGVMPVRAALQRLAAEG
                     AVVNVPHAGVAVPRLTVAEFDDLTQMRMLLEGEAAERGALRLDAEQRRQLQTLCERMQ
                     RALEHGRTRDYLSANEDFHGLLYRAAGSPTLFALIDTLWLKAGPVSNQLFETPEATDA
                     LNAAHEAALKALARADSSGVRRAIEQDIFVAAQFLRPRVRLAQD"
     gene            complement(3920..5302)
                     /locus_tag="VIN75_26650"
     CDS             complement(3920..5302)
                     /locus_tag="VIN75_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8977823.1"
                     /translation="MNTSPSAPRTGIAPGCVAGLLERERALFEQRNPRSRAVASQAAA
                     HLMFGVPLHWMSDWGTPFALQVSAANGATLADMDGHELVDFCLGDTGAMFGHSPPAVA
                     SALQRQAVRGLTAMLASEDAEAVGRLLSDRFGLPRWQFAMTATDANRFVLRWLRAATS
                     RPVVLVFNGCYHGTVDDAFVDLIDGRPVQRASLLGQVHDLTATTRVVEFNDLVALEAA
                     LAPGDVACVLAEPVMTNIGMVLPEPGFWERASELVRRHGSLLVMDETHTLSSGPGGYA
                     RAHGIVADAVVVGKALGGGMPCAAYGFSDDVARRAEGAKRAAAPGHSGIGTTLTANLL
                     AMAAMRATLSELLTPEVFAPMQARAARLAEDLRGIVARRGLPWCVTQVGARTELQFRA
                     APPRNGSEAAAAMDVELEHALHLYLLNRGVLITPFHNMMLVCPDTTDAHVERLCTTLD
                     AALGELGAAA"
     gene            complement(5304..6638)
                     /locus_tag="VIN75_26655"
     CDS             complement(5304..6638)
                     /locus_tag="VIN75_26655"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8977824.1"
                     /translation="MTSSLHEFLAAHRIHEVECVIADMTGIARGKILPRDLFLASGEM
                     RIPKSVLLNTVNGQQPDNGPFVGDTDPDMVCRPDARTVRIVPWAAEPVAVVIHDCEEW
                     DGQPVQLSPRAVLRRVLSLYERQGWQPVVAPEMEFYLVARNQNPHEPLQPPLGRTGKP
                     EVGRQSYSIDAVNDFDPFFMELSSFCDQHALGVETLIHEAGPGQMEINFSHGNALDLA
                     DRVFLFKRAVRETALRHGVFATFMAKPMEGEPGSAMHIHQSLVDADGSNLFSREDGGA
                     SDLFHRYIGGLQAYIPQVMPMFAPYVNSYRRLAPYMSAPINVRWGFDNRTCGIRIPKS
                     GPQARRVENRVPGVDANPYLAMAATLACGWLGMQMGLQPSAPTADSAWNVSHELPRHL
                     EDAIEAMRGCDAIKDVLGENFVDAFCAVKELEYATYNRVISSWEREHLLLLV"
     gene            complement(6888..7766)
                     /locus_tag="VIN75_26660"
     CDS             complement(6888..7766)
                     /locus_tag="VIN75_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8977825.1"
                     /translation="MDFTLQELSCLLSVASEGSFQAAAAKLHRTHPAVHSAIKQLEQR
                     VGVALLDRSQYRVRLTEAGQAFARQAGAVLEQARRLSDFSEQLTRGEESDLRVVIGDL
                     TPTTRLLPQLKRFFAAWPRTRLHLHFEALSGPWERLHAGDADLIVHHVDKSDARLEWV
                     GLMPVTLVPVAAPQGLPFAITRSLTPDRMREAVQVIIRDSARAASRDYFVIEGARNWT
                     VGDQHTKKELILRGMGWGHMPLHMVDKELRAGKLVSLEGRHFKRSRLEIVVARRADRQ
                     PGPVARALWDALAEPA"
     gene            7864..8844
                     /locus_tag="VIN75_26665"
     CDS             7864..8844
                     /locus_tag="VIN75_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8977826.1"
                     /translation="MKAIVRRGYGGPDQLEIRNVPDPQATAGEVTLRVRAFGINRAEQ
                     YFRQGLWGEVAPISGIECVGEVVVDPAGALLPGQRVMALMGGLGRTRSGSYAEMVNVP
                     AGNVVPVRSRLDWATLAALPETWATAWSCLHDNLGVRRGDRVLVRASTSALGQAALAL
                     ARHAGATVVASVRHVDRAGVALGKGAHHVLVEGRGLAGDLKALAPDGVDAVLDLVGTS
                     TLLESLQLARRGGRVCMAGFVGGGGPIERFDPLLHLPSGRQLSLFASAFVYGTADYPL
                     GEVPLQRIVDLVEAGVLDAAPARQFAFDQIRRVHELLDAEAAGGKMVVVV"
     gene            complement(8854..9606)
                     /locus_tag="VIN75_26670"
     CDS             complement(8854..9606)
                     /locus_tag="VIN75_26670"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8977827.1"
                     /translation="MASPAFAAVDDQVAPATPVVTASALAAGARRLVDDAMEKQRAVV
                     AVLRDNVVSPVMAGSWRLSVGKPYVPRATAPVTRPVPNDFADGALSGSAEADSLDLTL
                     GARWRVLHGDANSHVPMAAWMPDLQAADARASALRPTMNLSGEWALPNDFSLGVMPGM
                     AVDYNTLGRRQSNGTFAVTLGKTWSPQWRTFVDMARDRVAGVQIAGVSTSVDAGVTFV
                     ATPTTQIDFAVTRGISDTAPPFQAGVGVSSSF"
     gene            complement(10015..10815)
                     /locus_tag="VIN75_26675"
     CDS             complement(10015..10815)
                     /locus_tag="VIN75_26675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977828.1"
                     /translation="MRALAISTRFLAAVLALGACVAAHADPAAPRTDFLDSPAVIGLG
                     RWQYDPIFPLLPELHAPAVSRFASRFAALDVPVRGQAMQLRAAAGNSAVEAPLETAND
                     RLRRLHVFTDVSLGAQWRVRGGDAGWLPHVAWLADVETTGSPAFRDRNVRPSLRATAQ
                     WELPSQLSLGVMPGVYRDRGSDGKHYAAGVLAVTLGQAWTPRLQGFVELAGQCVSRVQ
                     PDASMLNLDTGLAFAASRSLHVDVVVSRSLSGSASQQLRGGLGVSSRF"
     gene            complement(10946..12241)
                     /locus_tag="VIN75_26680"
     CDS             complement(10946..12241)
                     /locus_tag="VIN75_26680"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY8977829.1"
                     /translation="MDSAVLLDLTTPREAAWRAALDRSGVHLGAASDRNKLVHCTIDG
                     GHGQLVCLAPGVSGELLSVLSCWNGEPPLAVLLLTPPGPMDAAQLRALVAGGIHHWQA
                     LEPDPAGEIAAVQVEAALAMAHARHEHDRALRLAATRAQEQLDERKWVDRAKGVLMTA
                     RGLDEDEAFRLLRGAAMSVNLRIGEVSRAVFEAARWADAMNRAGQLRMLSQRLVRLAA
                     QRLLRIDTRDAATQAVLSRQRARDNLDMLAAQCAGTVAAPACARAATCWDVLTQALQA
                     PRVGAADLRRIDAAATRMLDAAESLTDALQEAAGRRALHIVNQCGRQRMRAQRIAKQC
                     LLDAAHPEGADPRPRHDALLEAFELALVELEQAPLSSPAIRVTLAHVRDEWRRLLGGL
                     RAADTTDGRRALVQASEAILDRLDTLTAAYEHSLQVIMA"
     gene            12595..>13793
                     /locus_tag="VIN75_26685"
     CDS             12595..>13793
                     /locus_tag="VIN75_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8977830.1"
                     /translation="MTHFRKFLATGHAPTLFAAFLYFAFSCCTWVLNGAMAPFISESF
                     HLSPAQKGLMLSIPIIAGALFRFPLGVLSQYIGRKNATLVEMGLISVAMLLGFFFVQS
                     FDALLAMGVLLGVAGASFGVAMSLGSGSFPAEHKGLAMGIVGAGNVGTAVAVLIAPPL
                     AQWLGWQAVYAVAAAAMAIPICAVIFMAEEPKDIDPHAGFRAQVACLVERDGWAFSLI
                     YAVTFGGFIGFISFLPSYYRDQFGVSKVQAGQLTMLAAFMGAAMRVCGGWLSDRFGGI
                     NTLTVVLAAVALGLGACGLATGSLLATTVLLIACFAALGAGNGAVYQLVPLRWPLTTA
                     VAGSMIGELGALGGGLVPNAMGLARQYLGTYAWGFAFFALFALSTLALLRALQADWTS
                     RWVGAGGR"
BASE COUNT         2002 a         4747 c         4920 g         2124 t
ORIGIN      
        1 gaccccgccg ggactgccga acctgctggg ctgcgtttcc gcgccgcagg catggctgcc
       61 aatcggaagc gtctcgggct gtcggcggca gatttcggcc tcctggttgg cgccaccggc
      121 caatcgatct acgcttggga agcgggcaag gcaaagccca ggccgcaggc cttggcggcg
      181 attgcggccc tgcggggcgt cgggaaacgc gaagtcgaag ccaggctggc cggcctgaag
      241 ggcggaacag ctccggactg atggcgtcaa tcggcgctga gcgggcgcgc gccgcgtcca
      301 cctgacttca ccggccgctt gcatacgact tccgcagtgg ccggaatcat tcgacggccc
      361 gcgatttgct gctcttgcgt gtactgtcgc gccttggctg cgcgggcttg cccacgcggc
      421 gctcctctgc agccgcccaa gccgaagccc atgcggacaa gggttgcatc gcgacgcaca
      481 ggctgcgccc actggtcgtc aggtgatagc cgccctcgga atggtccacg aggttggagg
      541 cgcgcagctc cgtcaagcgc gtgttcagca agctggggtt ggtgtcgcaa gcttcctgca
      601 gcgcgcgaaa ggtcagtggt tcgccacgca gttcccaaag cacacgcaag gcgccgcggc
      661 gcccaagcag gtcgagcacg accatgatcg gccggcccgt cctggagcct ctgacattcg
      721 ttcctgcctt gggaatggcc atgctttact ttccatagcg cgcttgatat tctgctatga
      781 aaagtgtagc ttgaaagtcc acatggccgc gcgcatttcg cctctcgatc cgccctatga
      841 cgcgaaattg cagtgccggc tggatgccgt catgcatggc gctccgccgc tgcgactctt
      901 cacgaccctg gcacgcgact cccgcctgtt cgacaagttc ttcagcggat ccctgctgga
      961 tcgtggtcac ctgaccttgc gccagcgaga gatcgtgatc gaccggaccg cggcgctttg
     1021 cggttcggag tacgaatggg gcgtgcacgt ggccctgttt gctccgaagg cgcgcctcga
     1081 tgcggcggcg atccattcgc tcgcccacgg cagtgctgac gacccgtgtt ggtccgacga
     1141 ggatcgcgac ttgctgcgcc tgtgcgatgc acttcatcgg gactgtgacg tgggcgacga
     1201 gctttggcgg gcactgcaaa tgcgattttc ggaaggcgcc ctcctcgaat tgctcatgct
     1261 ggcaggcttc taccggaccg tcagctacct gaccaatgtc ttgcgtctgc cgctggagga
     1321 gggtgtcgcg cgctttccag gccctggtct gccgcccgcc ggggcgcaga ggttcgggtg
     1381 tgcttcgact cggccttcgc atggctcggg tgacgagcca ggagatgatc ttccgccggg
     1441 atcaagcggc tgacgccgtg cggcggttcc gcccgctttc atgcatccgc agatgtcgaa
     1501 ttgaacttgt ccaggaggcc aacccatgat caaggtcaac gtgatgtacc cgtacaccga
     1561 aggcgcgcgc ttcgatcatg cctactaccg cgaccggcat atgccgatga tgaaggccag
     1621 gctgggcagt gcctgcgcct actacaccgt cgaaaaaggc ctggccggca gagcgcccgg
     1681 cgcaccgccc gccttcgtgg ccatgtgcgc gttcatctgt gattcggccg aaggctacga
     1741 ggccgccctg caggagcacc gcgcagagat catggccgac atcgccaact acacggacat
     1801 cgcgccggtg ctccagttca gcgaggtcgt cgtcgagcgg tcggatcgct gaacatgccg
     1861 atcgacttcg aaacgccctg caattggctc gaaggacgcg catgactttc accctcgccg
     1921 gcttcgacct ggctgccttc gtcaacgcca cgctggccga agacctcggc atcggccttc
     1981 ccggtggcgg acgcgacatc acctcggaga gcgtcatccc ggcggacgcg cgctttgccg
     2041 gggtgatgga cagccgcgag gcgatcaccg tcgcagggct gccattggcc gccgcgttct
     2101 ttcgccatct cgacccggcg tgtgcgatcg aattgctggt gcaggatggc cagagcgtca
     2161 cgcccgggac cgcgctgatg cgaatcgccg gcaacgcccg cgcgctgctc accgccgaac
     2221 gcaacgcgct caataccgtc cagcacctct cgggcatcgc cacgatgacg cgcgcctatg
     2281 tcgaggcgat gcacggccgc gcgacgctgc tcgacacccg caagaccatt ccgggcctgc
     2341 gcgcgctgga gaagtacgcg gcacggatgg gcggcgccac caactaccga atgggcctgt
     2401 gggatgcggt gatgatcaag gacaaccatg tcgcggtggc gggcggcgtg gggccggccg
     2461 tggcgcgggc caaggcggcg ggcgtcgagc ggatcatctg cgaggtcgac cggatcgacc
     2521 agatcgagcc cgcgatcatg gccggcgcca cgcatatcct gctcgacaac atgacgccga
     2581 cgatgctttc agacgccgtg gcgctggtgg cgggccgtgc ggcaaccgag gccagcggcg
     2641 gcgtgcgtct cgacaacatc ggcgcggtcg cggcgagcgg agtggactac gtctcggtcg
     2701 ggcggctgat gcaaagcgcg cccgcggcgg atatcgggct ggatttcgag ctggtccgat
     2761 gaatggcggc tctcgagcga tgacttcgac aagcaaggcg accgcgacga gtggggctcg
     2821 acccgctcac gggtgtcgtc tgtcgatgcg tgtggcgcga gggcggctgc cgctgggttc
     2881 gcatgagccc gtgcgcgcgc gccgcgcaga cgatcgccgc gctcgcggcg gcaagccgtc
     2941 gcaagcgatc catcatccgt caacctcgca tcgttctcat cggccgatcg cggagtctgt
     3001 tgagggcgcg gatgtgattc gttccatgaa agcgaccgcg cgaagagaag cgctcgccat
     3061 ggccgcgatc ggccgggccg ccacgaggcg aattgcagcc gggagcggtc gttcactggt
     3121 gactggtttt cggatcaggc cgatgcggat gccaaggtcc gaaggctagt cctgtgcaag
     3181 gcgcacgcgc ggtcgcagga actgcgccgc gacgaagatg tcctgctcga tcgcgcgccg
     3241 aacgcccgaa ctgtcggcgc gggcaagcgc cttgagcgcg gcctcgtggg cggcgttgag
     3301 ggcgtccgtg gcctccggcg tctcgaacaa ctggttcgac accgggccgg ccttcagcca
     3361 cagcgtgtcg atcagcgcga acagcgtcgg cgagcccgcg gcgcgataga gcaaaccgtg
     3421 gaagtcctcg ttggccgaca ggtagtccct ggtgcgaccg tgctccagag cgcgctgcat
     3481 gcgctcgcac agcgtctgca actggcgccg ctgctccgcg tcgagcctca gcgcgccgcg
     3541 ttcagcagcc tcgccctcga gcagcatgcg catctgagtc aagtcgtcga actctgccac
     3601 cgtcaggcgc ggcacggcga cgcccgcgtg gggcacgttg acgacggcgc cttccgctgc
     3661 aaggcgctgc agcgccgcgc gcacgggcat cacgccgacg cccatctcgg cagcgacctg
     3721 ccggatcttc agccgctcgc cgggcacgaa tcggcccacc gtcacccatt ggcgcaggcg
     3781 gccgtagacc tcgtcctgct gggtcgtgac cgcttgccgg ccggtggtca tcacgtcctc
     3841 cacgccaagc cgaagtgagg catcacccgg gcgtcggtga ccgacatgat ccggcccctc
     3901 gggtcgacga gcgcgggcat catgcggccg ctcccaattc tcccagcgcc gcatccagcg
     3961 tcgtgcacag acgttcgacg tgagcgtcgg tggtgtcggg gcagaccagc atcatgttgt
     4021 ggaaaggcgt gatcagcacg ccgcgattga gcaagtacag atgcaacgcg tgctcgagct
     4081 cgacatccat cgcggcggcc gcctcgctgc cgttgcgcgg gggcgcggcg cggaattgca
     4141 gctcggtgcg agcgccgacc tgggtcacgc accacggcag gcctcgccgc gcgacgatcc
     4201 cgcgcaggtc ctcggccagt cgggcggccc gcgcctgcat cggcgcgaag acttccggtg
     4261 tcagcagctc ggagagcgtc gcgcgcatcg ccgccatcgc cagcaggttc gcagtcagtg
     4321 tcgtcccgat gccggagtgg cccggcgccg cggcgcgctt ggcgccctcg gcgcggcgag
     4381 cgacgtcgtc gctgaagccg taggccgcgc acggcatgcc accgcccagc gccttgccca
     4441 ccaccaccgc gtcggcgacg atgccgtgcg ccctcgcata gcccccgggg ccgctgctca
     4501 gggtgtgcgt ctcgtccatc accagcaagc tgccgtgccg tcgcacgagc tcgcttgcgc
     4561 gctcccagaa tccgggctcg ggcagcacca tgccgatgtt ggtcatgacc ggctcggcca
     4621 gcacgcacgc gacatcgccg ggcgccagcg cggcctcgag cgcgacgagg tcgttgaact
     4681 cgacgacgcg cgtcgtggca gtcaggtcgt gcacctggcc aagcaggctg gcccgctgca
     4741 cgggccggcc atcgatgagg tcgacaaacg catcgtcgac ggtgccgtgg tagcagccat
     4801 tgaacaccag caccacgggg cggctcgtcg cggcgcgcag ccagcgcagc acgaagcggt
     4861 tggcgtcggt ggccgtcatc gcgaactgcc agcgcggcag gccgaaccgg tcggacagca
     4921 gccggccgac cgcctcggcg tcctcgcttg cgagcatcgc cgtcaagccg cgcaccgcct
     4981 ggcgctgcag cgccgaggcg accgccggcg gcgaatggcc aaacatcgcg ccggtgtcac
     5041 ccaggcagaa gtcgaccagc tcgtggccgt ccatgtcggc gagcgtggca ccgttcgcgg
     5101 ccgagacctg cagcgcgaat ggcgtgcccc agtcgctcat ccagtggagc ggcacgccga
     5161 acatcaggtg cgcggcggcc tggctcgcca ccgcacggga acgcgggttg cgctgctcga
     5221 acagggcccg ttcccgctcg agcagaccgg cgacgcaccc gggcgcgatg ccggtgcgcg
     5281 gggctgaagg agatgtgttc atgtcagacg agcagcagca ggtgttcccg ctcccagctg
     5341 ctgatgacgc ggttgtaggt ggcgtactcg agttccttga ctgcacagaa cgcatcgacg
     5401 aagttctcgc ccagcacgtc cttgatcgca tcgcagccgc gcatggcctc gatcgcgtcc
     5461 tcgaggtggc ggggcagctc gtggctgacg ttccacgcac tgtcggcggt cggcgcagac
     5521 ggctgcaggc ccatctgcat gcccagccag ccgcacgcga gcgttgcggc catcgcgagg
     5581 tacgggttgg cgtcgacgcc ggggacgcgg ttctcgacgc ggcgcgcctg cgggccggac
     5641 ttcgggatac ggatgccgca ggtgcggttg tcgaagcccc agcgcacgtt gatcggcgcc
     5701 gacatgtagg gcgcgaggcg gcggtaggag ttcacgtacg gcgcgaacat cggcatcacc
     5761 tgcgggatgt aggcctggag gccgccgatg tagcggtgga acaggtccga cgcgccgccg
     5821 tcttcgcggc tgaacaggtt gctgccgtcg gcatcgacca ggctctgatg gatgtgcatc
     5881 gcgctgcccg gctcgccttc catcggcttg gccatgaagg tcgcgaacac gccatggcgc
     5941 agcgcggtct cgcgaaccgc gcgcttgaac aggaacacgc ggtcggccag gtccagcgcg
     6001 ttcccgtgtg aaaagttgat ctccatctgc cccggccctg cctcgtggat cagcgtctcg
     6061 acgccgagcg cgtgctggtc gcagaaggac gacagctcca tgaagaacgg atcgaagtcg
     6121 ttgaccgcgt cgatcgagta gctctggcgc ccgacttcgg gcttgccggt gcgccccagc
     6181 ggcggctgca gcggctcgtg cgggttctgg ttgcgcgcga cgaggtagaa ctccatctcc
     6241 ggcgcgacga ccggctgcca tccctggcgt tcatagagcg acagcacgcg gcgaagcact
     6301 gcgcgaggtg acagctgcac cggctggccg tcccactcct cgcagtcatg gatcaccacg
     6361 gcgaccggct cggccgccca cggaacgatt cgcaccgtgc gcgcatccgg cctgcagacc
     6421 atgtcgggat ccgtgtcgcc gacaaagggc ccgttgtccg gctgctggcc attgacggtg
     6481 ttcagcagca cgctcttggg gatgcgcatc tcgccactgg ccaggaacag atctcgcggc
     6541 aggatcttcc cgcgggcaat gcccgtcatg tcggcgatga cgcattccac ctcgtggatg
     6601 cggtgggcag ccaggaattc gtgcagcgag gatgtcatgc ctcgatcata gccgatgact
     6661 cgtgatgtga tcacaaatgg ccgaccgtta gccaacggga attcatagcg tcgtggcttt
     6721 gtgatcccat cttgaccgct ttttgcacgg acggccattg cctggccggg tgccggctgc
     6781 actgaaactc ctgcgcaatc gactgcgcgc tgcgtcaggc cgtcgcaccg tccgagtggg
     6841 gtgagtgggt ctcgtggcgg gcaacgcccc gagcggatcc aggagcgtca tgccggctcg
     6901 gcgagggcgt cccacagtgc acgggcgacc gggccgggct ggcggtcggc gcggcgtgcg
     6961 acgacgatct ccagtcgcga ccgcttgaag tggcggcctt ccagcgacac gagctttccc
     7021 gcgcgcagtt ccttgtcgac catgtgcagg ggcatgtgtc cccagcccat gccgcgcagg
     7081 atcagctcct tcttcgtgtg ctggtcgccg accgtccagt tgcgtgcgcc ctcgatcacg
     7141 aaatagtcgc ggctcgcggc gcgcgcggag tcgcggatga tgacctgcac cgcctcgcgc
     7201 atgcgatcgg gcgtcagcga acgcgtgatc gcgaacggca ggccctgcgg cgcggcgacc
     7261 ggcacgagcg tgacgggcat caggccgacc cattcgaggc gggcgtcgga cttgtcgacg
     7321 tggtgcacga tcaggtcggc gtcgccggcg tgaaggcgtt cccacggccc gctcagcgcc
     7381 tcgaaatgca ggtgcagccg cgtgcgcggc cacgccgcga agaagcgctt gagctgcggg
     7441 agcaggcgcg tggtcggcgt gaggtcgccg atgacgacgc gcaggtcgga ctcctccccg
     7501 cgcgtcagct gctcggagaa atcggacagg cggcgcgcct gttccagcac cgcgccggcc
     7561 tggcgcgcga acgcctggcc cgcctcggtc aggcgcacgc ggtactgcga ccggtcgagc
     7621 agggccacgc cgacccgttg ctcgagctgc ttgatggccg agtgcacggc cggatgcgtg
     7681 cggtgcaact tcgccgccgc ggcctggaag ctgccttccg aggccacgga cagcaggcag
     7741 gagagctctt gcagcgtgaa gtccattgtt cgtcgaatgt acagtcatcg gtcgaacttt
     7801 gaaatttcct tgttcgcggc gccatcggag actgcggcct ccaacacgac cgaggagatg
     7861 ttcatgaagg cgatcgttcg acgcggctac ggcggccccg accagctcga gatccgcaac
     7921 gtgcccgacc cgcaggccac tgcgggcgag gtgacgctgc gggtgcgggc gttcggcatc
     7981 aaccgcgcgg agcagtactt ccggcagggc ctgtggggcg aggtggcgcc gatctcgggg
     8041 atcgaatgcg tcggcgaggt cgttgtcgac ccggccggcg ccctcctgcc gggccagcgc
     8101 gtgatggcgc tgatgggcgg cctgggccgg acgcgcagcg gcagctatgc cgagatggtg
     8161 aacgtgccgg ccggcaacgt ggtgccggtg cgctcgcggc tggactgggc gacgctggcc
     8221 gcgctgcccg agacctgggc gaccgcctgg agctgcctgc acgacaacct gggcgtgcgg
     8281 cgcggcgacc gcgtcctggt gcgcgcgtcg acgtcggcgc tgggccaggc ggcgctcgcg
     8341 ctcgcgcgcc acgcgggcgc gaccgtcgtc gccagcgtgc gccatgtcga tcgcgccggc
     8401 gtcgcgctcg gcaagggcgc ccaccacgtg ctcgtcgaag gacgcggcct cgccggcgat
     8461 ctgaaggcac tggcgcccga cggcgtcgat gcggtgctgg atctcgtggg gacgtccacg
     8521 ctgctggaat ccctgcagct cgcgcgccgc gggggccggg tctgcatggc cggattcgtc
     8581 ggtggcggcg gaccgatcga acggttcgat ccgctgctgc acctgcccag cggacgtcag
     8641 ctcagcttgt ttgccagcgc gttcgtgtac ggcacggcgg actacccgct gggcgaggtg
     8701 ccgctgcagc gcatcgtcga cctggtggag gccggcgtgc tggacgccgc gcctgcccgc
     8761 cagttcgcgt tcgaccagat ccgccgcgtg cacgaactgc tcgacgccga ggcggcgggc
     8821 ggcaagatgg tggtggtcgt ctagtgtcgc gagtcagaag ctggaggaca cgcccacgcc
     8881 ggcctggaac ggcggcgcgg tgtcgctgat gccgcgcgtg acggcgaagt cgatctgcgt
     8941 ggtgggcgtg gcgacgaagg tgacgccggc gtcgacgctg gtggacacgc cggcgatctg
     9001 cacgccggcg acgcggtcgc gcgccatgtc gacgaaggtg cgccattgcg gcgaccacgt
     9061 cttgcccagc gtcaccgcga aggtgccgtt ggactggcgg cggcccagcg tgttgtagtc
     9121 gaccgccatg ccgggcatca cgcccagcga gaaatcgttg ggcagcgccc attcgccgct
     9181 gaggttcatg gtgggacgca gcgccgaggc gcgcgcgtcg gcggcctgca ggtcgggcat
     9241 ccaggcggcc atcggcacgt gcgagttggc gtcgccgtgc agcacgcgcc agcgtgcgcc
     9301 gagcgtgagg tcgagcgagt cggcctcggc actgccggac agcgcgccgt cggcgaaatc
     9361 gttgggcacg gggcgcgtga cgggcgcggt ggcgcgcggc acgtagggct tgcccacgct
     9421 caggcgccac gaaccggcca tgaccggcga gacgacgttg tcgcgcagca ccgcgacgac
     9481 cgcgcgctgc ttctccatcg cgtcgtcgac cagccggcgc gcgcctgccg cgagggcgct
     9541 ggccgtgacg accggcgtgg ccggcgcgac ctggtcgtcg acggcggcga aggcgggcga
     9601 ggccatggcg aacgccaggg cggcaaggag acggacgggg cgcatgaagg acggttccga
     9661 gacgacacag gacgcctggc gatcggcggg ttcacgcacc ggtcgcggcg tcctgcggga
     9721 gtgaagtcat cttgcgggat tgccgcgaag ggcgtcaacg caggagacgg tggaaacggt
     9781 gcctgttcgt ctcgtaacgt tacgcgaggt gactgatgag caggcccgcg cgcatgcgac
     9841 ggtctgcgtc cgggacggcg ggcggccacc cggcggggtg acggagcggg ccatggcgtg
     9901 aactggttca cgatgcgcag gccggttggc aggcccaagg ccgggcttgc cagaaccgcc
     9961 tggagcgcat cggcccgcgg accgcccgcg cgggcgctgc aaattgttgc ggcgtcagaa
    10021 gcgcgacgaa acgcccaatc caccgcgcaa ctgctgcgac gcgctccccg acaggctccg
    10081 cgacacgacc acatcgacat gcagcgaccg cgacgccgcg aacgcgagcc cggtatcgag
    10141 gttgagcatc gatgcatccg gctggacgcg cgacacgcac tggccggcca gctcgacgaa
    10201 gccctgcagc cgcggcgtcc acgcctggcc cagcgtcacg gccaggacgc cggcggcgta
    10261 gtgcttgccg tcgctgccgc ggtcgcggta cacgccgggc atcacgccca gcgacagctg
    10321 gctcggcagc tcccattgcg cggtcgcgcg cagcgagggg cgcacgttgc ggtcgcggaa
    10381 cgcgggcgag ccggtcgtct cgacgtcggc cagccacgcc acgtgcggca gccagccggc
    10441 gtcgccgccg cgcacgcgcc actgggcgcc gagcgagacg tcggtgaaga cgtgcagccg
    10501 gcgcagccgg tcgttggccg tctccagcgg cgcctcgacc gcgctgttgc ccgcggcggc
    10561 gcgcagctgc atggcctggc cgcgcaccgg cacgtcgagc gcggcaaagc gcgacgcgaa
    10621 gcgcgagacc gccggggcgt gcagctccgg cagcagcggg aagatcgggt cgtactgcca
    10681 gcggcccagt ccgatgacgg cgggcgaatc gaggaagtcg gtgcgcgggg ccgccgggtc
    10741 ggcgtgcgcg gccacgcacg cgcccagcgc cagcacggcg gcgagaaagc gggtggagat
    10801 ggccagggcg cgcaagatgg gcaccgtcct aacaagtcga tacatcggac ggaacgaccg
    10861 ttccgcggcg gatgggtcga tcttgcgggc gagcgcgcgc ggccgaaccg cgcatcagcg
    10921 ccgcaattgc cgcgcagttc gcgcgtcagg ccatgatcac ctgcaggctg tgctcgtagg
    10981 cggcggtcag cgtgtccagg cgatccagga tcgcctcgct ggcctgcacc agcgcgcgcc
    11041 ggccgtcggt ggtgtccgcc gcgcgcaggc cgccgagcag gcgccgccac tcgtcgcgca
    11101 cgtgcgccag cgtcacgcga atggcgggcg agctgagcgg cgcctgctcc agctcgacga
    11161 gcgcgagctc gaaggcctcg agcagcgcat cgtggcgcgg ccgcgggtcg gcgccttcgg
    11221 ggtgcgcggc atcgagcagg cattgcttgg cgatgcgctg cgcccgcatg cgctggcgcc
    11281 cgcactggtt gacgatatgc agcgcccgcc ggccggcggc ctcctgcaac gcgtcggtga
    11341 gggactcggc cgcgtccagc atgcgggtgg ccgcggcgtc gatgcggcgc aggtcggcgg
    11401 cgcccacgcg cggggcctgc agcgcctggg tcagcacgtc ccagcacgtg gccgcgcgcg
    11461 cgcaggccgg cgcggcgacc gtgcccgcgc attgcgccgc cagcatgtcg aggttgtcgc
    11521 gcgcgcgctg gcgcgacagc acggcctgcg tggcggcgtc gcgcgtgtcg atgcgcagca
    11581 gccgttgcgc ggccagccgc acgaggcgct gcgacagcat gcgcagctgg cccgcgcggt
    11641 tcatcgcgtc ggcccagcgc gcggcctcga acaccgcgcg cgacacctcg ccgatgcgca
    11701 ggttcacgct catcgcggcg ccgcgcagca ggcggaacgc ctcgtcctcg tcgagcccgc
    11761 gcgccgtcat cagcacgccc ttggcgcggt cgacccactt gcgttcgtcg agctgctcct
    11821 gcgcgcgcgt cgccgccagg cgcagcgcgc ggtcatgctc gtggcgcgcg tgcgccatcg
    11881 ccagcgcggc ctcgacctgc acggcggcga tctcgccggc cgggtccggc tccagcgcct
    11941 gccagtgatg aatgccgccg gccaccaggg cacgcagttg cgccgcgtcc atcgggcccg
    12001 gcggcgtcag cagcaggacc gcgagcggcg gctcgccgtt ccagcacgac agcaccgaca
    12061 gcagctcgcc gctcactccc ggcgccaggc agaccagctg cccgtgtccg ccgtcgatgg
    12121 tgcaatgcac cagtttgttg cgatccgatg ccgcacccag gtgcacgccg ctgcggtcga
    12181 gcgcggcccg ccaggccgcc tcgcgcggcg tggtgagatc gagcaggacg gcggaatcca
    12241 tggtgtggcg gggcgccgcc agagggcggc ggaggcttgt tggcgctcct gtccacttgc
    12301 acgtgccgtg ccattcgaaa tccggcacgg tgcttgcatt gagatgctcg cggtgcaacg
    12361 gcgcaccgcc cgtgaagggc cccgacccca ccggcctccc cggccgcccg tcgacgcgcg
    12421 agagcgctcg aaccgcttcc tgcccttcac cggttctcca tcgctgccga cacgcccacg
    12481 cagggcgcgc ttggccagcc caacgaactt tccccctttc gtccatcgtc ccgcaggcgt
    12541 tcgcgcgccg gcgtggccgc tgcctttgca accctttccc cggatgacgc cgccatgact
    12601 cacttcagga aattcctcgc gacaggccac gcgccgacgc tgttcgccgc gttcctgtac
    12661 ttcgcgttct cgtgctgcac ctgggtgctg aacggggcga tggccccttt catcagcgag
    12721 agcttccatc tcagtcccgc gcagaagggc ctgatgctgt cgatcccgat catcgcgggc
    12781 gcgctgttcc gcttcccgct gggcgtgctg tcgcagtaca tcggccgcaa gaacgccacg
    12841 ctggtggaga tggggctgat ctcggtggcg atgctgctgg gcttcttctt cgtgcaaagc
    12901 ttcgacgcgc tgctcgcgat gggcgtgctg ctgggcgtgg caggcgcgag cttcggcgtc
    12961 gcgatgtccc tcgggtcggg ctcgtttccg gcggagcaca agggcctggc gatgggcatc
    13021 gtcggcgccg gcaacgtcgg caccgccgtg gcggtcctga tcgcgccgcc gttggcccag
    13081 tggctcggct ggcaggcggt ttacgcggtc gccgcggcgg cgatggcgat cccgatctgt
    13141 gccgtcatct tcatggccga ggagcccaag gacatcgacc cgcacgctgg cttccgcgcg
    13201 caggtggcct gcctcgtcga acgcgacggc tgggcgttca gcctgatcta cgcggtgacc
    13261 ttcggcggct tcatcggctt catcagcttc ctgccctcgt actaccgcga ccagttcggc
    13321 gtcagcaagg tgcaggccgg ccagctcacc atgcttgcgg ccttcatggg cgcggcgatg
    13381 cgcgtgtgcg gcggctggct gtccgaccgc ttcggcggca tcaacaccct gaccgtggtg
    13441 ctcgcggccg tggcgctggg cctgggcgcc tgcggtctgg ccaccggctc gctgctggcc
    13501 accacggtgc tgctgatcgc gtgcttcgcc gcgctgggcg ccggcaacgg cgcggtctac
    13561 caattggtgc cgctgcgctg gccgctgacc accgcggtgg cgggctcgat gatcggcgag
    13621 ctgggcgcgc tcggcggcgg cctcgtgccc aacgcgatgg gcctcgcgcg ccagtacctg
    13681 ggcacgtatg catggggctt cgccttcttc gcgctgttcg cgctgtccac gctggcgctg
    13741 ctgcgcgccc tgcaggctga ctggacgagc cgctgggtcg gcgccggcgg tcg
//
LOCUS       DATCRO010000976         5024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_67409, whole genome shotgun sequence.
ACCESSION   DATCRO010000976 DATCRO010000000
VERSION     DATCRO010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67409"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1418
                     /gene="dalD"
                     /locus_tag="VIN75_26690"
     CDS             9..1418
                     /gene="dalD"
                     /locus_tag="VIN75_26690"
                     /EC_number="1.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
                     /protein_id="HEY8977831.1"
                     /translation="MSSPTNERLRLLHLGLGSFHRAHQAVYMHRLMQSGDTRWQVAGA
                     NLRPDMADTIAALQRQGGAYTLETVSPQGARRLEKITSITEVVPFEPTLLRVMALGAD
                     PATRIVSFTVTEAGYALDPEGQLDLRHEDIVNDLAGKPPRSIYGVIAALLRRRRAANA
                     GPLTLLNCDNLRHNGDRFRVNLRAFIAAADDAALLAWAGANTTCPNAMVDRITPRPTP
                     EVMARIRGLVGWDDQAPLMAESFIQWVIEDRFAAGRPEWEKVGVEMTGDVQPYEEAKI
                     RILNASHSAIAWAGTLSGLTYIHEGTLTPVIRDIAADYVTDDVIPCLARPGEPSPVDL
                     AAYRDVVLDRFSNDAIADTNQRVAADGFAKIPGFILPTIRERLARGESIAAVARLPAL
                     FLAFLQRWHQGRLPFAYQDQAMVPERAHAICDAVDPLSGFVAEAALWNELAGSPVLKD
                     AIAAARMDLHGLLGDAAMS"
     gene            1429..2358
                     /locus_tag="VIN75_26695"
     CDS             1429..2358
                     /locus_tag="VIN75_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8977832.1"
                     /translation="MHTPPRVRAPEIEHDHLRDPILGYDPPEAGGFLRCLAHGIPSPL
                     VRWHHHDEYELHLIMETSGKVFVGDYIGHFQPGHLVLTGPRVPHNWISSDAPPGGVAL
                     RDRVIQFAHEPIEQACELFPEFKEILPLLEQAKYGIEFFGVSEMAQAHFERVASSRGL
                     ARIAGFCEFMGMLARCKDFRLLSSVQLQCADDDESMAQVAAVVGYISEHCAETLSMAE
                     VSARFRMPESRFSRFFRRATGNTFTDFVNRMRISKACQLLMDTDRYVTTICYEVGFNN
                     VANFNRRFLEIKGMTPTDFRRQSASRFGLAETH"
     gene            complement(2431..2760)
                     /locus_tag="VIN75_26700"
     CDS             complement(2431..2760)
                     /locus_tag="VIN75_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977833.1"
                     /translation="MNAIVRLLAVYTGLSLLLCAFTRLEWSPLQPNGFGQWVALFLLI
                     FPAAAAAEFLGERLFGKGAPVRAEESMTHSDCSWGRVITGVGVLLVTFGVVLGAAWWL
                     SHPAASI"
     gene            complement(2931..4283)
                     /gene="miaB"
                     /locus_tag="VIN75_26705"
     CDS             complement(2931..4283)
                     /gene="miaB"
                     /locus_tag="VIN75_26705"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8977834.1"
                     /translation="MTKKVFIRTFGCQMNEYDSDKMADVLQAADGYEKTDDVEQADLV
                     LYNSCSVREKAAEKMFSDLGRIKHLKAKGVLIGVGGCVASQEGAAIVERAPYVDLVFG
                     PQTLHRLPKMIADRRAFSRPQVDISFPEIEKFDHLPPARVEGASAFVSVMEGCSKYCT
                     YCVVPYTRGEEVSRPFDDVLTEVAGLADQGLKEVTLLGQNVNAYRGRMGDSGEIADFA
                     LLLETIAEFPSIERIRFTTSHPKEFGQRLIDAYGRVPKLVNHLHLPVQHGSDRILSAM
                     KRGYTALEYKSTIRKLRAVRPDIRLSTDFIVGFPGEEDEDFDKLMKLVDDVGFDASFS
                     FIYSPRPGTPAANLLDPTASSVKLRRLQHLQAVLEANVLRFSEAMVGQTHRILVEGPS
                     RKSADDLMGRTECNRIVNFPGGPNAQRLVGQLIDVNVTAAYPHSLRAEVATTDSMAAA
                     "
     gene            complement(4379..4801)
                     /locus_tag="VIN75_26710"
     CDS             complement(4379..4801)
                     /locus_tag="VIN75_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977835.1"
                     /translation="MNRCAQLTFAATVAATLAALAPAPASAQATATDGQTLQRNFPKE
                     ALRGNIVFFAPPAIALNGVNTQMAPAYRIHGTNNMLIMSGQLNGLKATVDYTTDVQGA
                     VREVWILTPAEIARTWPTTAAQAAAWTFDAIAQTWTKP"
BASE COUNT          764 a         1770 c         1722 g          768 t
ORIGIN      
        1 accgcaccat gtcgtccccg accaacgagc gcctgcgcct gctgcacctg ggcctgggct
       61 ccttccatcg cgcgcaccag gcggtctaca tgcaccggct gatgcagtcc ggcgacaccc
      121 gctggcaggt cgccggcgcc aacctgcgtc ccgacatggc cgacaccatt gccgcgctgc
      181 agcgccaggg cggcgcctac acgctggaga cggtgtcgcc gcagggcgcg cgccggctcg
      241 agaagatcac gtccatcacc gaggtcgtgc cgttcgagcc gacgctcttg cgcgtcatgg
      301 cgctcggcgc ggatccggcc acgcgcatcg tctcgttcac ggtgaccgag gccggctacg
      361 cgctcgaccc cgagggccag ctcgacctgc ggcacgagga catcgtcaac gacctcgccg
      421 gcaagccgcc gcgctcgatc tacggcgtga tcgccgcgct gttgcgccgc cgccgcgcgg
      481 ccaacgcggg cccgctgacg ctgctcaact gcgacaacct gcgccacaac ggcgaccgct
      541 tccgcgtcaa cctgcgcgcg ttcatcgccg cggccgacga cgcggcgctg ctggcctggg
      601 ccggcgcgaa cacgacgtgt cccaacgcga tggtcgaccg catcacgccg cgcccgacgc
      661 ccgaggtgat ggcgcgcatc cgcgggctcg tcggctggga cgaccaggcg cccctgatgg
      721 ccgagtcctt catccagtgg gtgatcgagg accgcttcgc ggccggccgc cccgagtggg
      781 agaaggtcgg cgtggagatg accggcgacg tgcagcccta cgaggaggcc aagatccgca
      841 tcctcaacgc gagccacagc gcgatcgcct gggccggcac gctcagcggc ctgacctaca
      901 tccacgaggg cacgctcacg ccggtgatcc gcgacatcgc cgccgactac gtcaccgacg
      961 acgtgatccc gtgcctggcg cggcccggcg agccgagccc ggtcgacctg gcggcctacc
     1021 gcgacgtcgt gctcgatcgc ttctccaacg acgccatcgc cgacaccaac cagcgcgtgg
     1081 cggccgacgg ctttgccaag atccccggct tcatcctgcc gacgatccgc gagcggctgg
     1141 cacgcggcga gagcatcgcc gcggtggcgc ggctgccggc gctgttcctg gccttcctgc
     1201 agcgctggca ccagggccgg ctgccgttcg cctaccagga ccaggcgatg gtcccggagc
     1261 gcgcgcacgc catctgcgac gccgtcgatc cgctgtcggg cttcgtcgcc gaggccgcgc
     1321 tgtggaacga gctggcgggc agccccgtgc tcaaggatgc cattgcggcg gcccgcatgg
     1381 atctgcatgg tttgctcggc gatgccgcca tgagctaagg tgagaaccat gcacacgccg
     1441 ccccgcgtcc gcgcccccga gatcgagcac gatcacctgc gcgaccccat cctcggctac
     1501 gacccgcccg aggcgggcgg cttcctgcgc tgcctggcgc acggcatccc gtcgccgctg
     1561 gtgcgctggc accaccacga cgagtacgag ctgcacctga tcatggagac ctcgggcaag
     1621 gtcttcgtgg gcgactacat cggccacttc cagcccggcc acctggtgct gaccgggccg
     1681 cgcgtgccgc acaactggat ctccagcgac gcgccgcccg ggggcgtcgc gctgcgcgac
     1741 cgcgtcatcc agttcgcgca cgagccgatc gagcaggcct gcgagctgtt ccccgagttc
     1801 aaggagatcc tgccgctgct cgagcaggcc aagtacggca tcgagttctt cggcgtgtcc
     1861 gagatggcgc aggcgcactt cgagcgcgtc gcgtccagcc gcggcctggc gcgcatcgcc
     1921 ggcttctgcg agttcatggg catgctcgcc cgctgcaagg acttccgcct gctgtccagc
     1981 gtgcagctgc aatgcgccga cgacgacgag tcgatggcgc aggtggcggc cgtggtgggc
     2041 tacatcagcg agcactgcgc cgagacgctg tcgatggccg aggtcagcgc gcgcttccgc
     2101 atgcccgaga gccgcttctc gcgtttcttc cgccgcgcga ccggcaacac gttcaccgac
     2161 ttcgtcaacc gcatgcgcat cagcaaggcc tgccagctgc tgatggacac cgatcgctac
     2221 gtgacgacga tctgctacga ggtcggcttc aacaacgtgg ccaacttcaa ccgccgcttc
     2281 ctcgagatca agggcatgac gccgaccgat ttccggcgcc agtcggcatc gcgcttcggg
     2341 ctcgccgaaa cgcactagac gcgcgttccc gaagcctgta tcccttgccg caggaaagct
     2401 tcgggcggcc gctcccgccg ccgccgccgt tcagatcgac gcggccggat gcgacagcca
     2461 ccacgccgcg ccgagcacga cgccgaaggt gaccagcagc acgcccacgc cggtgatgac
     2521 gcggccccac gagcagtcgc tgtgggtcat gctctcctcg gcccgcaccg gggcgccctt
     2581 gccgaacagc cgctcgccga ggaactcggc ggccgcggca gcgggaaaga tgaggaggaa
     2641 gagggcgacc cactgcccga agccgttcgg ctggagcggc gaccactcca ggcgcgtgaa
     2701 cgcgcagagc agcaacgaca gacccgtgta gaccgcgagc aagcggacga tggcgttcat
     2761 gcttgcgaac tccattcaag acccaaccgg atcagcatag ggcaatttcc gtgcgacgtg
     2821 gatgaagcga agccttccgc gacccccggg ccaacccgga ggaacgcgag gttcgtcacg
     2881 catccctggg ggaactacct aggtttgcat cgggtgcgcc ggcgccgcgc tcaggccgcc
     2941 gccatcgagt ccgtcgtcgc cacttccgcc cgcagcgaat gcggatacgc cgccgtgacg
     3001 ttcacgtcga tcagctgccc caccaggcgc tgcgcgttcg ggccgccggg gaagttgacg
     3061 atgcggttgc actcggtgcg gcccatcagg tcgtcggcgc tcttgcggct cgggccctcg
     3121 accaggatcc tgtgggtctg gccgaccatc gcctcgctga agcgcagcac gttggcctcc
     3181 agcacggcct gcaggtgctg caggcgcctg agcttgacgg acgacgccgt cgggtcgagc
     3241 aggttggccg ccggcgtgcc ggggcgcggg ctgtagatga acgagaacga cgcgtcgaag
     3301 ccgacgtcgt cgaccagctt catgagcttg tcgaagtcct cgtcctcctc gccggggaag
     3361 ccgacgatga agtcggtcga caggcggatg tcggggcgca ccgcgcgcag cttgcggatc
     3421 gtgctcttgt actcgagcgc cgtgtagccg cgcttcatcg cggacaggat gcggtccgag
     3481 ccgtgctgca cgggcaggtg caggtggttc acgagcttgg gcacgcggcc gtaggcgtcg
     3541 atcaggcgct ggccgaattc cttggggtgg ctggtcgtga agcggatgcg ctcgatgctg
     3601 gggaactcgg cgatggtctc cagcagcagc gcgaagtcgg cgatctcgcc cgagtcgccc
     3661 atcctgccgc ggtacgcgtt gacgttctgg cccagcagcg tcacttcctt gaggccctgg
     3721 tcggcgaggc cggccacctc cgtcagcacg tcgtcgaacg ggcgcgagac ttcctcgccg
     3781 cgcgtgtatg gcaccacgca gtaggtgcag tacttggagc agccttccat caccgagacg
     3841 aacgcgctgg cgccttcgac gcgcgcaggc ggcaggtggt cgaacttctc gatctccggg
     3901 aagctgatgt cgacctgcgg gcgcgagaac gcgcggcggt cggcgatcat cttgggcagg
     3961 cggtgcagcg tctgcgggcc gaacacgagg tcgacatagg gcgcgcgctc gacgatcgcc
     4021 gcgccttcct ggctcgccac gcagccgccc acgccgatca gcacgccctt ggccttcagg
     4081 tgcttgatgc gcccgaggtc ggagaacatc ttctcggcag ccttctcgcg caccgagcag
     4141 ctgttgtaca ggacgaggtc ggcctgctcg acgtcgtccg tcttctcgta gccgtcggcg
     4201 gcctgcagga cgtcggccat cttgtccgag tcgtactcgt tcatctggca gccgaacgtg
     4261 cggatgaaga ccttcttggt catggaactc tcctgcgccg gtaacgcgcg cgggcctcgt
     4321 cgcgaggccg ttgaaatcga atggggatgc ctggggccgc gtgccgtcgc ggcacgcgtc
     4381 agggcttggt ccaggtctgg gcgatggcgt cgaaggtcca ggcggcggcc tgggctgccg
     4441 tcgtcggcca ggtccgggcg atctcggccg gcgtgaggat ccagacctcg cgcacggcgc
     4501 cctgcacgtc ggtggtgtag tcgaccgtgg ccttgagccc gttcaactgg cccgacatga
     4561 tcagcatgtt gttggtgccg tggatgcgat aggccggcgc catctgcgtg ttgacgccgt
     4621 tcagggcgat cgcgggcggc gcgaagaaga cgatgttgcc gcgcaaggct tccttgggaa
     4681 agttgcgctg cagggtctgg ccgtcggtcg ccgtggcctg ggccgacgcc ggggccggcg
     4741 cgagcgcggc gagcgtggcc gcgacggtcg cggcgaaggt gagctgggca cagcggttca
     4801 tggtgtagat ccaggggctg cgaatgaaaa ggcccgaggc atgcccgggc ccgctgcgcg
     4861 cgcggatctc gcgatccggc gcaggctgca ttctaagggc gcgcgccccg gcgctggcgt
     4921 cagaaaccgg ccttctgcat cacgaacagc aggatcagcg cgccgaccac cgacagcacc
     4981 aggccggccg ggtgcacgat ggcgccgtcc gccggcttgc tgaa
//
LOCUS       DATCRO010000977         7538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_978668, whole genome shotgun sequence.
ACCESSION   DATCRO010000977 DATCRO010000000
VERSION     DATCRO010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978668"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VIN75_26715"
     CDS             complement(<1..100)
                     /locus_tag="VIN75_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY8977836.1"
                     /translation="MSEFKVAPANASAGVKLLTWVDNRFPATKLWKE"
     gene            complement(182..793)
                     /gene="petA"
                     /locus_tag="VIN75_26720"
     CDS             complement(182..793)
                     /gene="petA"
                     /locus_tag="VIN75_26720"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY8977837.1"
                     /translation="MSESVVVHEGIDDSKRKWVAITCVAGAGAGIATAVPFVSTFAPS
                     ERAKAAGAAVEVDISDLKAGEKKTVEWRGQPVWIVKRSPEMVAALSKDTAELADPESK
                     RNPDELTPPYARNEHRSIKSDVLVVVGICTHLGCSPGDKFSAGAQPSLPADWPGGWLC
                     PCHGSTFDLAGRVFKNKPAPDNLRVPPHMYLSDSKLLIGEDKA"
     gene            1047..1466
                     /gene="mscL"
                     /locus_tag="VIN75_26725"
     CDS             1047..1466
                     /gene="mscL"
                     /locus_tag="VIN75_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8977838.1"
                     /translation="MSFTSEFKEFAVKGNVMDLAVGVIIGAAFGKIVDSVVNDLIMPL
                     VGKATGGLDFSNYFVPLAGQTATNLADAKKAGAVFAYGNFITVAINFVILALIIFVMV
                     KQINKLKKETPPPVAPPAPTPEDVLLLREIRDSLKAR"
     gene            complement(1521..2573)
                     /gene="pdxA"
                     /locus_tag="VIN75_26730"
     CDS             complement(1521..2573)
                     /gene="pdxA"
                     /locus_tag="VIN75_26730"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8977839.1"
                     /translation="MPSHPRPASGPSRAALPLLVTMGDAAGIGPEIIAKAAAAGELDD
                     AVVLADPGVMRRAVAACGLLLPVASLEHPRDRAACPPGTLAVCAPPGLGDGLAELPLG
                     AIDARAGAAAARCIEQAVAWVRDDAGRAIVTAPIHKEALSAAAVPFPGHTEMLQALAT
                     APGAAPPPVRMMLANDELRVVLVTIHLSLRRAIDALTEQGIVDTLAITHEAGLRFGLA
                     APRIAVAGLNPHAGEGGLFGDEEITLIAPAIARARAQGIAASGPYAPDTVFMRARDAA
                     GKPGEFDFVVAMTHDHGLIPVKYLGVEQGVNVTLGLPFVRTSPDHGTAFDIAGRGIAD
                     PASLVAAARMARRLAP"
     gene            2642..4516
                     /locus_tag="VIN75_26735"
     CDS             2642..4516
                     /locus_tag="VIN75_26735"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8977840.1"
                     /translation="MTLPSTAGLGRPATLALLALCAPLAFAQGAGFAAARSPLAPAPG
                     DAKRFHATLEATRFRQLLDNDWQWRMEQFPEQATRLGDHRYDDRLTDESPEAVARREQ
                     HQREQLKALKRIDVDVLTGEDRISYDVALQDAQLAVDWQKYPAMHTRVLSAKDGVQLG
                     LPSLMEDFPVRTEADARHAIARLKAMPQRIAQDIAWLREGKRVGWVTFKASLSQVPDQ
                     IDKLLAKPAAEQPLLAPFNRLPDDIPADHRAALLDEATGVLQSQVAPAFQELRKMVVD
                     ELLPASPEDGAMSAYPDGEAVYKLFMRAQTTLPLDARAVHQIGLDEMARIHKEIDATM
                     AQAGFTGTYAEFAAQLWHDPKWYYTSGDELLAGYRDIAKRIDPQLTRLFITLPRTPYG
                     IRAIPAYQGANTVEFYTPGAADASTPGWFNANAVAIAQRPKWDMVDLFLHEGVPGHHL
                     QISRAHELQALPMFRRAALVNAYVEGWALYAESLGNELGLYDDPYQRYGYLRAQAWRA
                     GRLVVDTGIHALGWTRAQAIDYLVDSGGLAREDATAEVDRYFVWPGQALGYKLGEIKI
                     RELRQKARDALAERFDLRAFNQEILDHGALPLPVLDHVISAWIDRTRNGATPALADGR
                     "
     gene            4516..5700
                     /locus_tag="VIN75_26740"
     CDS             4516..5700
                     /locus_tag="VIN75_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8977841.1"
                     /translation="MRRLWLLFAQAVTVTLAVVAVLAAWKPQWWGSMRARELAAASAP
                     APARVGAVVEAPSLAPPGASTNSFAAAVRRAAPAVVSITASKTGLRRPAAGGGAIWPF
                     GGNGRNQAQTGLGSGVLVSSDGYLLTNQHVVDGADDIEVTLSDGRSAGAKVVGTDADS
                     DLALLKVSLDNLPVIPFGDIRHLSVGDPVLAIGNPFNVGETVTSGIVSALDRNQLGLS
                     TIENFIQTDAAINPGNSGGALVDGEGRLVGINTAIFSRSGGSMGIGFAIPVDIAREVM
                     DALIRDGEMTRGWIGVQPRDLTPEFADSFQLPVKEGVLVTGVLRDGPAAQAGLKPGDV
                     VTAIGGVKVSNTAQLLRAVGALKPPSSAVLAVQRGSDAMTLSVPVAKRPAAKPRDVDD
                     GR"
     gene            complement(5812..6594)
                     /gene="tatC"
                     /locus_tag="VIN75_26745"
     CDS             complement(5812..6594)
                     /gene="tatC"
                     /locus_tag="VIN75_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8977842.1"
                     /translation="MATPPEDELAGTEQPFVAHLVELRDRLLRSVWGVAIIFAALCAW
                     PGPSRLYDYLARPLIASLPAGAHMIAVGVVSPFLIPIKVTVLVATGIALPWVLYQIWA
                     FVAPGLYKHEKRLVAPLVVTSTLLFYSGVAFSYFIVFQKAFPAIQRMAPASVQVSPDI
                     EAYLDFVLTMFIAFGVAFEVPVAVVIVARLGLVTVEQLRKFRNYYWVVAAGVAGLVTP
                     PDPASMIALLVPMILLYEVGIWTVPIFIRRTQAPDAETKKEA"
     gene            complement(6677..7153)
                     /gene="tatB"
                     /locus_tag="VIN75_26750"
     CDS             complement(6677..7153)
                     /gene="tatB"
                     /locus_tag="VIN75_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY8977843.1"
                     /translation="MFDIGVTKLAIVGGIALIVIGPERLPRVARMVGTMLGRAQRYVA
                     DVKAEVNRSIELEELNKMKSQFEGAAREVHDTVQRTMRDPGAFDEPGMSDVSDAAAMR
                     ALQPQPAYKPPRKNWRLKRSAIPAWYKQRAGVRTNAQSGAARVARFRPRSRGGEAI"
     gene            complement(7190..7420)
                     /gene="tatA"
                     /locus_tag="VIN75_26755"
     CDS             complement(7190..7420)
                     /gene="tatA"
                     /locus_tag="VIN75_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY8977844.1"
                     /translation="MGLSTTHLIIFLVIIVLIFGTKKLKNIGSDLGSAVKGFKDGMKD
                     GEKPEEARPPQQVTATDARASNDTIDVEAKNR"
BASE COUNT         1125 a         2651 c         2683 g         1079 t
ORIGIN      
        1 gttccttcca gagcttggtc gcggggaagc ggttgtcgac ccaggtcagc agcttgacac
       61 cggcagaggc gttggcgggg gctaccttga attcagacat gcggtcctct tgtcttgatc
      121 cagcgcgccg ggctcgcatt caggccgggt cgcgatgtct cggaatcttg gaaactcggg
      181 atcaggcctt gtcctcgccg atcagcagct tgctgtcgct caggtacatg tgcggcggca
      241 cgcgcaggtt gtccggcgcg ggcttgttct tgaacacgcg gccggccagg tcgaacgtgg
      301 aaccgtggca ggggcacagc cagccgccgg gccagtcggc cggcagcgag ggctgcgcgc
      361 cggcggagaa cttgtcgccg ggcgagcagc ccaggtgcgt gcagatgccg accacgacga
      421 gcacgtcgga cttgatcgag cgatgctcgt tgcgcgcgta cggcggcgtc agttcatcgg
      481 ggttgcgctt ggactcgggg tcggccagtt cggccgtgtc cttggacagc gcggccacca
      541 tctcggggga ccgcttgacg atccacacgg gctggccgcg ccattccacc gtcttcttct
      601 cgcccgcctt caggtcggag atgtccactt cgacggcggc tcccgccgcc ttggcccgct
      661 cggacggggc gaaggtgctg acgaacggca cggccgtcgc gatccctgct cccgctcccg
      721 ccacgcacgt gatggccacc cacttccgtt tgctgtcgtc gataccttcg tgcactacaa
      781 cgctttcact catgagcatc ctcgagctga agttccagtt ccgggcaacg cctttgagtt
      841 tagcgtgcgc aaccgcacgt tcgagtcacc ccggcaccac aggcaaaccc ttggtacgcc
      901 aattggcgca cgggcgtcga agggccggta ttgacgcccg tcaacggcgg gcaatactgc
      961 gaaccaggca atgcaggcgg ggggtttctc gcatgcgggc ccgcggtccc gaatcaatca
     1021 aacataccta ggcaggagtt cgaaaaatga gtttcacaag tgaattcaag gagttcgccg
     1081 tcaagggcaa cgtcatggat ctcgcggtcg gcgtgatcat cggcgcggcc ttcggcaaga
     1141 tcgtcgactc ggtcgtcaac gacctgatca tgccgctcgt gggcaaggcc acgggcggtc
     1201 tcgacttctc gaactacttc gtcccgctcg cgggccagac ggccaccaac ctggccgacg
     1261 cgaagaaggc cggcgcggtg ttcgcctacg gcaacttcat caccgtggcg atcaacttcg
     1321 tgatcctggc gctgatcatc ttcgtgatgg tcaagcagat caacaagctc aagaaggaga
     1381 cgccgccgcc ggtcgcgccg cccgcgccga cgcccgagga cgtcctcctg ctgcgcgaga
     1441 tccgcgactc gctgaaggcg cgctgagccc gcgcgccggg gcaaccggag ccgcccgccc
     1501 gcgagggccg gcggcttttc tcatggcgcc aggcgccgcg ccatccgcgc ggccgcgacc
     1561 aggctggccg ggtcggcgat gccgcggccg gcgatgtcga acgcggtgcc gtggtcgggg
     1621 ctggtgcgca cgaagggcag gcccagcgtc acgttgacgc cctgctccac gccgaggtac
     1681 ttgacgggga tcagcccgtg gtcgtgcgtc atcgcgacga cgaagtcgaa ctcgccgggc
     1741 ttgcctgcgg cgtcgcgggc gcgcatgaag acggtgtcgg gcgcgtaggg gccgctggcg
     1801 gcgatgccct gcgcgcgggc gcgcgcgatc gccggggcga tcagcgtgat ctcctcgtcg
     1861 ccgaacagcc caccttcgcc cgcgtgcggg ttcaggccgg cgacggcgat gcgcggcgcc
     1921 gcgaggccga agcgcagccc ggcctcgtgc gtgatggcca gcgtgtcgac gatgccctgc
     1981 tcggtgagcg cgtcgatggc gcgccgcagc gacaggtgga tggtcaccag caccacgcgc
     2041 agttcgtcgt tggccagcat catgcgcacc ggcggcggcg cggcgcccgg cgcggtggcc
     2101 agcgcctgca gcatctcggt gtggccgggg aacggcacgg cggcggccga cagcgcctcc
     2161 ttgtggatcg gcgcggtgac gatcgcgcgg cccgcgtcgt cgcgcaccca ggccaccgcc
     2221 tgctcgatgc agcgcgccgc ggccgcgccg gcgcgcgcgt cgatcgcgcc caggggcagc
     2281 tcggccaggc cgtcgcccag gcccggcggc gcgcacacgg ccaacgtgcc gggcgggcac
     2341 gccgcgcgat cgcgcgggtg ctcgagcgac gccaccggca gcagcagacc gcaggccgcg
     2401 accgcacggc gcatcacgcc cggatcggcc agcacgacgg cgtcgtcgag ctcgccggct
     2461 gcggcggcct tggcgatgat ctcggggccg atgccggccg cgtcgcccat ggtgacgagc
     2521 agcggcagcg cggcgcgcga cggcccggaa gcggggcggg ggtgcgaggg catccacaga
     2581 ttgttccacg ggcgccgtgc gtgccacact cgtcgcttcg atgcccctcc aacgcctcgc
     2641 cgtgactctt ccatcgaccg ccggactcgg ccgccccgcc accctcgcgc tgctggcgct
     2701 gtgcgccccg ctcgcgttcg cgcaaggcgc cggtttcgcg gccgcccggt cgccgctcgc
     2761 gcctgcaccc ggcgacgcca agcgcttcca cgcgacgctc gaggccacgc gctttcgcca
     2821 gttgctggac aacgactggc aatggcgcat ggagcagttt cccgagcagg ccactcgcct
     2881 gggggaccat cgctacgacg accgcctgac cgacgagtcg cccgaggccg tggcgcggcg
     2941 cgaacagcac cagcgcgaac agctcaaggc gctcaagcgc atcgacgtcg acgtgctcac
     3001 cggcgaggac cgcatctcgt acgacgtcgc gctgcaggac gcgcagctgg ccgtcgactg
     3061 gcagaagtac ccggcgatgc acacgcgcgt gctctcggcc aaggacggcg tgcagctcgg
     3121 gctgccgtcg ctgatggagg acttccccgt gcgcaccgag gccgatgcgc ggcacgcgat
     3181 cgcgcgtctg aaggccatgc cgcagcgcat cgcgcaggac atcgcgtggc tgcgcgaggg
     3241 caagcgcgtg ggctgggtca cgttcaaggc ctcgctgtcg caggtgcccg accagatcga
     3301 caagctgctc gccaagcccg ccgccgaaca accgctgctc gcgccgttca accggctgcc
     3361 ggacgacatc cctgccgacc accgcgcggc gctgctggac gaggccaccg gcgtcctgca
     3421 gtcgcaggtc gcgcccgcat tccaggagct gcgcaagatg gtggtcgacg agctgctgcc
     3481 cgccagcccc gaggacggcg cgatgagcgc ctatcccgac ggcgaggccg tctacaagct
     3541 gttcatgcgc gcgcagacca ccctgccgct ggacgccagg gccgtgcacc agatcggcct
     3601 ggacgagatg gcgcgcatcc acaaggagat cgacgcgacg atggcgcagg ccggcttcac
     3661 cggcacctac gccgagttcg ctgcgcagct gtggcacgac ccgaagtggt actacacgag
     3721 cggcgacgaa ctgctcgcgg gctaccgcga catcgccaag cgcatcgacc cgcagctgac
     3781 caggctgttc atcacgctgc cgcgcacgcc ctacggcatc cgcgcgatcc ccgcctacca
     3841 gggcgccaac acggtcgagt tctacacgcc gggcgcggcc gacgcgtcca cgccgggctg
     3901 gttcaacgcc aacgccgtgg cgatcgcgca gcggccgaag tgggacatgg tcgacctgtt
     3961 cctgcacgag ggcgtgccgg gccaccacct gcagatctcg cgcgcccacg agctgcaggc
     4021 gctgccgatg ttccgccgcg ccgcgctcgt caacgcgtac gtcgagggct gggcgctgta
     4081 cgccgagagc ctgggcaacg agctgggcct gtacgacgat ccgtaccagc gctacggcta
     4141 cctgcgcgcg caggcctggc gcgccggccg gctcgtggtc gacaccggca tccacgcgct
     4201 gggctggacg cgcgcgcagg ccatcgacta cctcgtcgac agcggcggcc tggcgcgcga
     4261 ggacgccacc gcggaggtcg accgctactt cgtctggccg ggccaggcgc tcggctacaa
     4321 gctgggcgag atcaagatcc gcgagctgcg ccagaaggcg cgcgacgcgc tcgccgagcg
     4381 cttcgacctg cgcgcgttca accaggagat cctcgaccac ggcgcgctgc cgctgccggt
     4441 gctggaccac gtgatcagcg cgtggatcga tcgcaccagg aacggggcga cgccggcgct
     4501 cgcggacggc cggtgatgcg caggctctgg ctgctgttcg cgcaggcggt caccgtcacg
     4561 ctggccgtcg tcgcggtgct ggccgcctgg aagccgcagt ggtggggctc gatgcgcgca
     4621 cgcgagctcg cggccgcgtc cgcgccggcg cccgcgcgcg tgggcgccgt cgtcgaggcg
     4681 ccgtcgctcg cgccgcccgg cgcgtcgacc aacagcttcg ccgcggccgt gcggcgcgcg
     4741 gcgccggcgg tggtgtcgat cacggccagc aagacggggc tgcgccggcc cgcggcgggc
     4801 ggcggcgcga tctggccgtt cggcggcaat ggccgcaacc aggcgcagac ggggttgggc
     4861 tcgggcgtgc tggtgtcgtc cgacggctac ctgctgacca accagcacgt ggtcgacggc
     4921 gccgacgaca tcgaggtgac gctgtccgac ggccgctccg ccggcgccaa ggtggtgggc
     4981 accgacgccg actccgacct cgcgctgctg aaggtgtcgc tggacaacct gccggtcatc
     5041 ccgttcggcg acatccgcca cctgtcggtg ggcgacccgg tgctggcgat cggcaacccg
     5101 ttcaacgtgg gcgagacggt cacctcgggc atcgtcagcg cgctcgatcg caaccagctg
     5161 gggctctcga cgatcgagaa cttcatccag accgatgcgg ccatcaaccc gggcaactcc
     5221 ggtggcgcgc tggtcgacgg cgaaggccgg ctggtgggca tcaacacggc gatcttctcg
     5281 cgcagcggcg gcagcatggg catcggcttc gcgatccccg tggacatcgc ccgcgaggtg
     5341 atggacgcgc tgatccgcga cggcgagatg acgcgcgggt ggatcggcgt gcagccgcgc
     5401 gacctcacgc ccgagttcgc cgacagcttc cagctgccgg tcaaggaagg cgtgctggta
     5461 acgggcgtgc tgcgcgacgg ccccgccgcg caggcaggcc tgaagccggg cgacgtggtg
     5521 accgccatcg gcggggtgaa ggtgtccaac accgcgcagc tgctgcgcgc ggtcggcgcg
     5581 ctcaagccgc cgtccagcgc ggtgctggcg gtgcagcgcg gcagcgacgc gatgacgctg
     5641 agcgtgccgg tggccaagcg gccggcagcc aagccgcggg atgtcgacga cggccgctga
     5701 ccaggtcggc gctcacatgt gtccggcggc gagccgtgtc gtgggcatgt cgcgcggcgt
     5761 ccggtacgcc aatggcgtca tcctgcgcgc agtcgcagga tccacgcggg ctcaggcttc
     5821 cttcttcgtc tccgcgtccg gcgcctgcgt gcgcctgatg aagatcggca ccgtccagat
     5881 gcccacctcg tacagcagga tcatcggcac cagcagcgcg atcatcgacg ccgggtcggg
     5941 cggcgtcacc aggccggcga cgccggcggc gaccacccag tagtagttgc ggaacttgcg
     6001 cagctgctcc accgtcacga ggcccaggcg cgccacgatc accaccgcca ccggcacctc
     6061 gaacgcgacg ccgaacgcga tgaacatcgt cagcacgaag tcgaggtagg cctcgatgtc
     6121 cgggctcacc tgcacggagg ccggcgccat gcgctggatc gccgggaacg ccttctggaa
     6181 cacgatgaag tagctgaacg ccacgcccga gtagaacagc agcgtgctgg tgaccacgag
     6241 cggcgccacc aggcgcttct cgtgcttgta gagccccggc gcgacgaagg cccagatctg
     6301 gtacagcacc cagggcagcg caatgccggt ggccaccagc accgtcacct tgatcgggat
     6361 caggaacggc gacacgacgc ccacggcgat catgtgcgcg cccgcgggca gcgacgcgat
     6421 cagcgggcgc gcgaggtagt cgtagagccg gctcgggccg ggccaggcgc acagcgcggc
     6481 gaagatgatg gccacgcccc agacgctgcg cagcaggcgg tcgcgcagtt cgacgaggtg
     6541 ggcgacgaag ggctgctccg tgccggccag ttcgtcttcg ggtggggtcg ccatgggtca
     6601 gcggctctcg gagggttgga cgagggagcc gcggacgacc gcgacccggc agggccgcgc
     6661 gccgccggcg gcgcggtcag atcgcttcac cgccgcggct gcgcgggcgg aagcgcgcca
     6721 cgcgcgccgc gcccgactgg gcgttcgtgc gaacacccgc gcgctgcttg taccaggccg
     6781 ggatggcgct gcgcttcagg cgccagttct tgcgcggcgg cttgtaggcc ggctgcggct
     6841 ggagcgcgcg catcgcggcg gcgtccgaga cgtcggacat gcccggctcg tcgaacgccc
     6901 cggggtcgcg catcgtccgc tggacggtgt cgtgcacttc gcgggcggcg ccctcgaact
     6961 gcgacttcat cttgttgagt tcttccagct cgatcgagcg gttgacctcg gccttgacgt
     7021 cggcgacgta gcgctgcgcc cggcccagca tcgtgccgac catgcgcgcg acgcgcggca
     7081 gccgctcggg gccgatcacg atcagggcga tgccgccgac gatggccagc ttggtgacgc
     7141 cgatgtcaaa cattcacggg atcgtcgagt ggtcgcgccg gacgcgcgtt cagcggttct
     7201 tggcctcgac gtcgatggtg tcgttcgacg cgcgggcatc ggtggcggtg acctgctggg
     7261 gcggacgcgc ctcttccggc ttctcgccgt ccttcatgcc gtccttgaag cctttgacgg
     7321 ccgagccgag atccgagccg atgttcttca gcttcttcgt gccgaagatc agcacgatga
     7381 tgacgaggaa gatgatgagg tgggtggtag aaagacccat gacgggctcc ttgcaatgtc
     7441 aggcgcgggg cgcaacgcgc ggcacgcgcg tgagacatcc attctaaggg accgggggac
     7501 ggcggccttc cggccaaatc aaaggccatt cacccctt
//
LOCUS       DATCRO010000978         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_78631, whole genome shotgun sequence.
ACCESSION   DATCRO010000978 DATCRO010000000
VERSION     DATCRO010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78631"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VIN75_26760"
     CDS             <1..363
                     /locus_tag="VIN75_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977845.1"
                     /translation="MTSRAPPPRFEPCAFDGVRYDAAYASSDAAPRTRSGGVVAIDEA
                     TGRALWTVVLWTTPVEDAQGLFHPPRFLRRVSRGDEAGELRVEDEHGVLYVVDLATRA
                     FRTVEPPRKPASFPRPPE"
     gene            363..1466
                     /locus_tag="VIN75_26765"
     CDS             363..1466
                     /locus_tag="VIN75_26765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEY8977846.1"
                     /translation="MVDWNRKFRSEYDAAREVLGSPEAFEPDWQPVVAQLARLMGLDG
                     FDVSRADALDELRRRATHGGGVERVDEAEGLLRAARSGADGGLVADAVRARAAALKLL
                     RHVYLEAHAGGRGVWVVALPSEFTDWPSTQFSDEATTPAGARQLLAGRGEHFDARERR
                     WLGAAAQQGLAWCQRASMVLADARRARANAPAERERARALVRRWFAEPEAAEGAVDKL
                     VATLARGFKDIVAALNRGRFIITDWVPLRAASTGLEAEFLRTEAFAFRARSEGMDVVY
                     VEGAFFDDVAGNVLCGQANWTRILVHELSHLVCGTHDVNDGQSRYAWAGIGPHSGYPS
                     AETIRNADNWAFFAADCAGALTAGQRATALRKT"
BASE COUNT          318 a          722 c          735 g          281 t
ORIGIN      
        1 atgaccagcc gcgcaccgcc gccccggttc gaaccctgcg ccttcgacgg cgtgcgctac
       61 gacgcggcct atgcgtccag cgacgcggcg ccgcgcacac gttcgggggg cgtggtcgcc
      121 atcgacgagg ccaccggccg cgccctgtgg acggtggtct tgtggacgac ccccgtcgag
      181 gacgcccaag gcctgttcca ccccccgcgc ttcctgcggc gcgtgagccg cggcgatgag
      241 gccggcgaac tgcgcgtcga ggacgagcac ggcgtgctct acgtcgtcga cctcgccacg
      301 cgcgcctttc gcaccgtgga gcccccgcgc aagccggcca gcttcccgcg gccgcccgag
      361 tgatggtcga ctggaatcgc aagttccgca gcgagtacga cgccgcgcgc gaggtgctgg
      421 ggtcgcccga ggcgttcgag ccggactggc agccggtcgt cgcgcagctc gcgcgcctga
      481 tgggcctgga cggcttcgac gtcagccgcg ccgatgccct cgacgaactg cgccgccgcg
      541 ccacgcatgg cggcggcgtc gagcgcgtgg acgaggcgga gggcctgctg cgcgcggcga
      601 gatcgggcgc ggacggtggc ctggtcgccg acgccgtccg agcgcgcgcc gcggcgctca
      661 agctgctgcg ccacgtctat ctcgaggcgc acgcgggcgg acgcggcgtc tgggtcgtgg
      721 cgctaccttc cgagttcacc gactggccca gtacccagtt ctccgacgag gccaccacgc
      781 ccgcgggcgc gcgacaactg ctggcaggac ggggcgagca cttcgacgcg cgcgaacgcc
      841 gctggctcgg cgcggccgcc cagcagggcc tggcctggtg ccagcgcgcg agcatggtgc
      901 tggccgacgc gcgccgcgcg cgggcgaatg cgcccgccga acgcgagcgg gcacgcgcgc
      961 tcgtacggcg ctggttcgcg gagcccgaag ccgccgaagg cgccgtcgac aagctcgtcg
     1021 ccacgctggc gcgcgggttc aaggacatcg tcgccgcgct caaccgcggc cgcttcatca
     1081 tcaccgactg ggtgccgctg cgcgccgcga gcaccggcct ggaggccgaa ttcctgcgca
     1141 cggaggcctt cgcgttccgc gcgcgcagcg aaggcatgga cgtcgtctat gtcgagggcg
     1201 cgttcttcga tgacgtggcg ggcaacgtgc tctgcggcca ggcgaactgg acgcgcatcc
     1261 tcgtgcacga gctgtcgcac ctggtctgcg gcacgcacga cgtcaacgac ggccagtcgc
     1321 gctacgcctg ggcgggcatc ggcccgcact cgggctatcc gagcgcggag acgatccgca
     1381 acgccgacaa ctgggcgttc tttgcggctg attgcgccgg ggcgctcacc gcggggcagc
     1441 gggcgacggc gctgcgcaag acctgatcga cgcgcgacga tcgtcgaacg aaggtggcac
     1501 gtggatggtt gaaccaccac gccacgtgcc acgccagccg aagctgcacc ggtgaaagga
     1561 cgcgacccga tctcaggaga agggtgcgtt gaacaaaacc cgtgtcctgc cactggacca
     1621 cctgcccccc aaacgcacgc gggatgcatg cgcttgggtg ggcaggacag gaatcgaacc
     1681 tgtgccacgg gttcggacga cgtttgcacg ccgcccagtc aggcccgcct tgggatgacc
     1741 gatgctcggg aagacaactt tgtcgaggcg aaacgagaaa gccaaggatc gtgtgtcgag
     1801 gggagcatgc cccgcagccg tattccaatt ccggcacgtc cttgccaagc aaactcgcgc
     1861 gcggcacgcg gccgcgatgg gattcgcttg gtaaggacgg gtggattcga accaccaacc
     1921 ctgcgggtct gccaccgacg acaccttgac gagaaggata agggtgcctc tgggcctccc
     1981 gctgccggga ggcggattct tcgaatgtcc gacgatgctc agcggaaggc ctgcatcagg
     2041 tgcgacgcga ttgcgc
//
LOCUS       DATCRO010000979         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_382460, whole genome shotgun sequence.
ACCESSION   DATCRO010000979 DATCRO010000000
VERSION     DATCRO010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382460"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..647)
                     /locus_tag="VIN75_26770"
     CDS             complement(42..647)
                     /locus_tag="VIN75_26770"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977847.1"
                     /translation="MHGSYVSPNATLGEGVKIGRFCIVEDGVTLADGVVLEDYAMVQA
                     GARIGARTKIGTYTKIGRDVRIGEDCSFTSFCEIRDRCDLGDRVLMGSRGTLSAGTVV
                     EDEVVMKYAFVVTDTPDLQKNNEKSVGRLKRKSRFGASVVIMPSVTIGENAEIGACSQ
                     VRKDVPDNEVWFGTPAKYYRDVDAAAPAPATAPVRKLALAR"
     gene            complement(660..1607)
                     /locus_tag="VIN75_26775"
     CDS             complement(660..1607)
                     /locus_tag="VIN75_26775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977848.1"
                     /translation="MTTRHGPFALDAILAALDALGLAFSVHGRRDVGPLRFCSLFAQE
                     PQGLYFWEGDGVASFEHADIAVLTARALPGDIACQIVLAHPQRAFYLLMRHFFAPDAP
                     AETAIHPTAVIDPRATIGAGTTIGPYCVIGAATVGAGCHFDSHVVLKDGAVVGDRVTI
                     ESHSTIGATGVAWVWDPHTGERIVQPQIGGARIGDDCFIGTDVTVVRGSVNEVTSLGE
                     GCVVAHGTKIGHGCVIGAHVHFANNVSIAGNCRLGDRSFLSAASVLRPRIVLARGVVV
                     GAGAVVTRNVDVEGVVVSGVPARIMDAKASHAGVPKLPA"
     gene            complement(1610..2611)
                     /locus_tag="VIN75_26780"
     CDS             complement(1610..2611)
                     /locus_tag="VIN75_26780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY8977849.1"
                     /translation="MPTITTLIPAYKKEYLGETLLGLARQTFRDFRVVISDDSPDDEI
                     THLIRSGHYGDLSRSLDLEVVRGPKNARLNHQSLLDRWAGSSPFVHLQLDDDVVFPEF
                     YRTHMAAHRTGRFSASVSRRWVTHGGLMPVMGINQPAPVAQSPLLHVPVDADTLFRTM
                     VPTCNNWIGEFTHMVMSAEGARAYPRPPADDLSYYGWLDVGFVLSAVQVAPLVIVRDH
                     LGIFRQHPSQTTNRLNTSIGRVSSLAWVTTALRAWREGRITHADVVTALKWNIGQCLE
                     RFGETDAMINESFDIIQQHGANLEHLYAAWRPFWLRLLAQHPATSPCARPLREAAAA"
     gene            2868..>3271
                     /locus_tag="VIN75_26785"
     CDS             2868..>3271
                     /locus_tag="VIN75_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8977850.1"
                     /translation="MAASRKPIYVTQPELPPLEEFMPYLRQIWDTRVLTNGGPFHQML
                     ESALCDHLGVRHISLFTNATIALVTALQSLRITGEVITTPYSFVATAHSLMWNGIKPV
                     FVDVDPVTLNMDPAKIEAAITPQTTAIMPIHC"
BASE COUNT          494 a         1095 c         1199 g          483 t
ORIGIN      
        1 gccgcgggag gccgcggccg gcgtcgacgg atggggtgaa cttagcgcgc gagcgcgagc
       61 ttgcgcaccg gcgcggtcgc cggcgcgggc gcggcggcgt cgacgtcgcg gtagtacttg
      121 gccggcgtgc cgaaccacac ctcgttgtcg ggcacgtcct tgcgcacctg cgagcacgcg
      181 ccgatctcgg cgttctcgcc gatcgtcacc gagggcatga tcacgacgct ggcgccgaag
      241 cgcgacttgc gcttgaggcg gccgacgctc ttctcgttgt tcttctgcag gtcgggcgtg
      301 tcggtgacga cgaaggcgta cttcatcacc acctcgtcct cgaccaccgt gccggccgac
      361 agcgtgccgc ggctgcccat caggacgcgg tcgccgaggt cgcagcggtc ccggatctcg
      421 cagaacgagg tgaacgagca gtcctcgccg atgcgcacgt cgcggccgat cttggtgtac
      481 gtgccgatct tcgtgcgcgc gccgatgcgg gcgccggcct gcaccatcgc gtagtcctcg
      541 aggacgacgc cgtcggccag cgtgacgccg tcctcgacga tgcagaagcg gccgatcttg
      601 acgccctcgc cgagcgtggc gttgggggag acgtaggaac cgtgcatggg aagtcctttt
      661 caggcgggaa gcttgggaac gcccgcgtgg ctggccttgg cgtccatgat ccgggcgggc
      721 acgcccgaga ccacgacgcc ttccacgtcc acgttgcggg tgacgacggc gccagctccg
      781 acgacgacac cgcgcgcgag cacgatgcgc ggcctgagca cgctggcggc gctcaggaag
      841 ctccggtcgc cgaggcggca attgccggcg atggagacgt tgttggcgaa atgcacgtgc
      901 gcgccgatga cgcagccgtg gccgatcttg gtgccgtggg cgacgacgca gccttcgccc
      961 agcgaggtga cctcgttgac ggagccgcgc accacggtga cgtcggtgcc gatgaagcag
     1021 tcgtcgccga tgcgcgcgcc gccgatctgc ggctgcacga tgcgctcgcc ggtgtgagga
     1081 tcccagaccc acgcgacgcc ggtggcgccg atggtggagt gcgactcgat ggtgacgcgg
     1141 tcgccgacga cggcgccatc cttgaggacg acgtgcgagt cgaagtggca gccggcgccc
     1201 acggtggcgg cgccgatgac gcagtacggc ccgatggtcg tgccggcgcc gatggtggca
     1261 cgcgggtcga tgacggccgt cgggtggatc gcggtctcgg ccggcgcgtc gggcgcgaag
     1321 aagtggcgca tcagcaggta gaaggcgcgc tgcgggtgcg cgagcacgat ctggcaggcg
     1381 atgtcgccgg gcagcgcccg ggcggtgagc acggcaatgt cggcgtgctc gaagctcgcc
     1441 acgccgtcgc cttcccagaa gtaaaggccc tggggctcct gcgcgaacag gctgcagaag
     1501 cgcagcgggc cgacgtcgcg ccggccgtgc acgctgaatg ccaggcccag cgcgtccagc
     1561 gcggccagga tggcgtcgag cgcgaagggg ccgtggcggg tcgtcatggt caggcggcgg
     1621 ccgcctcgcg caggggccgg gcgcagggcg acgtggcggg atgctgggcc agcaggcgca
     1681 gccagaacgg gcgccacgcg gcgtagaggt gctcgaggtt cgcgccgtgc tgctggatga
     1741 tgtcgaacga ctcgttgatc atcgcgtccg tctcgccgaa gcgctccagg cactggccga
     1801 tgttccactt caatgcggtc accacgtcgg cgtgcgtgat ccggccttcg cgccacgcgc
     1861 gcagggccgt cgtcacccag gccagcgacg acacgcggcc gatgctggtg ttcaggcggt
     1921 tcgtggtctg cgacgggtgc tggcggaaga tgcccaggtg gtcgcgcacg atcacgagcg
     1981 gcgcgacctg cacggcgctc agcacgaagc cgacgtccag ccagccgtag tacgagaggt
     2041 cgtccgccgg cggccgcgga taggcccgcg cgccctcggc cgacatgacc atgtgcgtga
     2101 attcgccgat ccagttgttg caggtgggca ccatcgtgcg gaacagcgtg tcggcgtcca
     2161 ccggcacgtg cagcagcggg ctctgcgcca ccggcgcggg ctggttgatg cccatcaccg
     2221 gcatcagtcc gccgtgggtc acccagcggc ggctcaccga ggcgctgaag cggcccgtgc
     2281 gatgcgcggc catgtgggtg cggtagaact cggggaagac gacgtcgtcg tcgagctgca
     2341 ggtgcacgaa cggcgagctg ccggcccagc gatccagcag cgactggtgg ttcagccggg
     2401 cgttcttcgg gccgcgcacc acctcgagat ccagcgagcg cgacaggtcc ccgtagtggc
     2461 cggaccggat gaggtgggtg atctcgtcgt ccgggctgtc gtcggagatc acgacgcgga
     2521 agtcgcggaa ggtctggcgc gcgaggccca gcaacgtctc gcccaggtat tccttcttgt
     2581 aggcaggaat gagggtggtg atcgtcggca tgcgggtgtc cagggctttc tcgggcggat
     2641 gagccgatga tcggcgcaga ccgtgcccgg cgatcgccgg aactgcggcc ccgatgcacg
     2701 ccagatcgcg ccttcagcgc ggcggcccgc cgcgggcccg tgccttcctg cggccaattg
     2761 acgccccggg gccggccatt tccgcggatc gtcgaggtgc ccgcgcggcc atgatcgagc
     2821 cacggacgcg cggcatgccc gagccgccgc cgaacgaaag acgctccatg gccgccagcc
     2881 gcaagcccat ctacgtgacc cagccggaac tgcctccgct cgaggagttc atgccctacc
     2941 tgcggcagat ctgggacacg cgcgtgctga ccaacggcgg ccccttccac cagatgctgg
     3001 aatcggcgct gtgcgaccat ctgggcgtcc gccacatcag cctgttcacg aacgcgacca
     3061 tcgcgctggt gaccgcgctg cagtcgctgc gcatcacggg tgaagtgatc accacgccgt
     3121 actcgttcgt cgcgaccgcg cactcgctga tgtggaacgg catcaagccg gtcttcgtgg
     3181 acgtcgaccc ggtgacgctg aacatggacc cggcgaagat cgaggcggcg atcacgcccc
     3241 agacgacggc catcatgccg atccactgct a
//
LOCUS       DATCRO010000980        12061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_854940, whole genome shotgun sequence.
ACCESSION   DATCRO010000980 DATCRO010000000
VERSION     DATCRO010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854940"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIN75_26790"
     CDS             complement(<1..286)
                     /locus_tag="VIN75_26790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8977851.1"
                     /translation="MTRAGAFARVGVPTLSVAAFLLAWEGAVRALHIPAYLLPAPSLV
                     ATTLVDNFASLAASWWFTVRITLIALASAMAGGVLIAALFALSRTAERALA"
     gene            complement(283..1065)
                     /locus_tag="VIN75_26795"
     CDS             complement(283..1065)
                     /locus_tag="VIN75_26795"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8977852.1"
                     /translation="MNSRSPSAALSLAQVRRVYSGGRVALADVSLRIAPGEFVSLLGA
                     SGCGKSTVLRLLSGLDAPSAGTITRGVPGDEIPGYVFQDAALLPWASVRRNVELPLML
                     RGERPAAMRERVDAALRAVGLSDSANAVPRELSGGMRMRASLARALVTSPRLWLLDEP
                     FGALDEITRFALNQTLLALCKPAGGAAPATAVFVTHSVYEAVFLSERVLVMARPGRIA
                     EEIAIDVPYPRDAGFRATPEFAALCARVSAALQQANVAGGDA"
     gene            1410..2462
                     /locus_tag="VIN75_26800"
     CDS             1410..2462
                     /locus_tag="VIN75_26800"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8977853.1"
                     /translation="MNTIHHRAPIRLMGLCVAVGAAFAGTAHAEAITLYGVADVYIEA
                     GKGDRHEISLNSGGLSGSRLGFDASRELGDGLKAVAKLEAGYALDHGTSTQGGILFGR
                     QAYAGLSGSFGTVTAGRQYTPQFLALDANDPFDTGAGSAVSSGIVSLFASRANNSVVW
                     SAPSSGGFAVTAMVALGESNTGSQTNSTLASVDLHYGSGSSGVNLTFTGVKKPSDDGV
                     NASAVTLGGTYDFGSLKLMGGMQWVRNATGAPDTADNRREFFAGVNVPFGADALWLGA
                     GTGKTQGVAGSTASQYSIGYVHSVAKGVDVYGVLTDIQNGSATAYSDDTATGAGPAVS
                     AGKSASAAQVGFRYRF"
     gene            2606..3529
                     /locus_tag="VIN75_26805"
     CDS             2606..3529
                     /locus_tag="VIN75_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8977854.1"
                     /translation="MLSFDTVSIASRLIPALLCAALAAPATAQESITLSKIRESGVIT
                     LGYRDSQFPFSYLDDAQRPIGYTMDICDRIVDAVQARLGLRDLERRYVQVTSATRIPM
                     VSNGTVDLECGVTTNTAERERDVAFTITTFVAESRLLSKKSSPVWRLDDLRGKVVTST
                     VGTTSLAHLQDLYARHGIDMSILASRDDPEAFRLVATDRAAAYAMDDALLRGTIARSG
                     QPDDYVVSNERLAVEPYGIMLVKGDPEFKRLADQVIIGLFRSGEILQLYRRWFQSPTP
                     PRGINLAFPIFPSMLRLVARPTDSPDPANYP"
     gene            complement(3531..4883)
                     /locus_tag="VIN75_26810"
     CDS             complement(3531..4883)
                     /locus_tag="VIN75_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8977855.1"
                     /translation="MFDGYKVALIDDDPAVRRSLAQTLELAGLEVVAHASAEEGLATL
                     GAGFLGVVVTDVRLPNMDGMALMKRALQIDRALPVILITAHGDVHLAVEAMRAGAYDF
                     LEKPFAPERLVDVVTRAMERRALSAEVQSLRLQLAARRGIDATLLGRSPAMKVLQRQV
                     QQLAATNCDVMIIGETGTGKELVARCLHDHSARRDRHFVAINCGGLPESLLESELFGH
                     EAGAFTNASRKRIGKFEHADGGTLLLDEIESMPMSFQVKLLRVLQERKVERLGSNDEI
                     PVDVRVIAATKADLRLLSEQQLFRSDLHYRLNVAVLRLPPLRERREDVPLLFEHFMLE
                     ASARHAREPEPLSDAMSQQLMSHDWPGNVRELRNAAERFVLGLSDDTVLTTGDAPVLT
                     LAQQLDQVEKVLIERALKHHQGRMSVTCEALGIGRKTLYDKIARLHIDVSGYRGAERP
                     "
     gene            complement(4886..6553)
                     /locus_tag="VIN75_26815"
     CDS             complement(4886..6553)
                     /locus_tag="VIN75_26815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977856.1"
                     /translation="MRLDDGVAHQLDLYAAVLDQELGNHADLPGMLDQDDHTVALIDS
                     PDNTALRSALNRRLTRFVARSGALSASVVDAKAHVLASSNWYRADTRGTSRAAPEPCV
                     VEALGGGLPVQRFEPNLDNGAPEVCFARPVRRDGATVGVVIVRISLQPTEATWIDSAF
                     RAESEKPLIVDSRGIVILSSVPAWKQQALSTLTQAQGQTGSDVKLVRVPALKATGMEM
                     QVVHERPLPRMGWRLIILTNARDVWREARAAAWGAGAIAASLGLLLLLLLQRRRVAVQ
                     KVAARAALQRAHDELETKVLQRTAQLESSNASLRHEVQERQHAEQVLRQAQEELVQAG
                     KLALLGQLSAGISHELGQPLTALRALAQNGCLLLERERPLQARDNFKAILGLAERMGR
                     ITAQLKSVARKAPAEVRSLPIVGAIDNARQILAARFSAESVALVTDIPPGLAARCDNY
                     RLEQVFVNLMANAMDAMRATEARRLTIAAAASADRAVVRVSDSGPGIPHELNEHLFEP
                     FFTTKAPGEGLGLGLVISSQIVREFGGTLRAVPSTSGAVFEFDVALG"
     gene            6973..7929
                     /locus_tag="VIN75_26820"
     CDS             6973..7929
                     /locus_tag="VIN75_26820"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8977857.1"
                     /translation="MHTQALQRVRRSVNDKIDHRILHVLQKDGRISNLQLAEQVHLSP
                     SAVFERVRRLQREGYILGYQARLSHEKLGMEVVAFVEVQLERMNPQVRQHFEDAVRQR
                     AAILECYMIAGTFDYLLKVVVSDMHACRELVASVISRLPGVREARTHAGIEQPRPPVP
                     APAGAEPFIDDVDAALLRLLQADGRRSTARLAEELDLTAAVVRERLSRLCRRGFILGY
                     TAVLNEAKLKSGLQVFAAVRTAAAATGIARVLRAAALNHVEILECHDVTGNFQHLLKL
                     RVADMRSHNELMESAVWPLPGVCEVRTYAVIDEIKNTTRIPL"
     gene            7929..9080
                     /locus_tag="VIN75_26825"
     CDS             7929..9080
                     /locus_tag="VIN75_26825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8977858.1"
                     /translation="MNNTFFPCAAILAGIGGLLAAAGCGRVPDVVKIGVAQPLSNGLA
                     PLGQDMVNGAQMAIDEINAANGVDVGGKRVRLALVTADDKGANEAGDAAARKLVDEDV
                     EVAIADLNSGVSIHAAPIYAAAGIPQLAISTKPEFTRLGLPTTLRLVASDDLQSKALG
                     AYAAQIPGASRYAVVDDGTPYGQGLAEAAAKVIEGRGGHVGVRRTLDDRTVDFGTLVA
                     ALAADKSDLIVTTLSDFQVDALMAQLARAGLTQMIIVGGDTIKTDKLARKAIPIKAVY
                     ATSSIVEPREFPGGKLFLKNFRAKFKADPVYGAHYAYDAVYVVADALARNRSVDKAAL
                     LKTLKDFDGNCPMTNSMRFGADGEQRYAAVAVYQLHDGAWDPLMRSDRW"
     gene            complement(9145..>12061)
                     /locus_tag="VIN75_26830"
     CDS             complement(9145..>12061)
                     /locus_tag="VIN75_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY8977859.1"
                     /translation="VVESSATVDVDPHRVDIVLPEDFVMPPGGVHIRWPDPPLEQEAR
                     LMDFKWYAALAYVRANKLNHNVVSTPDDRFGLIASGKAFNDLRQALADLGLDDDTCRS
                     LGIRIHKVNVVWPLEATITRDFARGLKEILVVEEKRQMIEYQLKEQLYNWRNDVRPTV
                     LGKFDEQEGDLSGGEWSNPNPGDNWLLRPKADLTPAIIAKAVARRLKKLGVPADIVRR
                     MDERIAVIEAKDRALAALKAGSSSGVGDRTPWFCSGCPHNTSTKVPEGSRAVAGIGCH
                     YMVNWMDRSTSTFTQMGGEGVPWVGQAPFTTEKHIFANLGDGTYYHSGLLAIRQSLSS
                     NVNITYKILFNGAVAMTGGQPVDGQIDVASTTRELEAEGVRRIVVVSDEPERYNSGVR
                     LADGVTVHHRDQLDAIQRELREVPGVTVILYDQTCATEKRRRRKRGTLAANPRRVVIN
                     EAVCEGCGDCSVQSNCLSVEPLETEFGRKRRINQNTCNQDFSCVNGFCPSFVSVEGTL
                     KKPKPAKAKDAPALPEIPLPTLPAASTAWGIVVAGIGGTGVITIGQLLGMAAHLEGKG
                     VVTQDAAGLAQKGGATWSHIQIADTQDAILTTKVDAAKADLVIGCEAIVAANPVTLGV
                     MREGRTYVALNTHRSPTASFVNDPDWQFPGGKCETAIEAAVGKDLLGAFDADTVATRL
                     LGDSIFTNPLMLGYAWQQGRVPLSHAALMRSIELNGVQVEKNKQAFEWGRRCAHDLAS
                     VQAQFAAAQVLSFVKKPSLVETVQRRVDFLTGYQDADYAALYATIVDRVKAAEAPLAS
                     TRLSETVARNLFKLMAYKDEYEVARLHTDPAFLAKLSDQFEDGFRLKYHLAPPLLAAK
                     DGKGHLVKKTYGGWMQRAFGVLAKFKGLRGTAFDVFGYTAERRTERALIAEYRELIDE
                     LLGALTVDRLADAVALAALPDEIRGYGHVKERNLEKVRAKWAGQLAAWRANERAEAA"
BASE COUNT         1813 a         4228 c         4161 g         1859 t
ORIGIN      
        1 gcgccagcgc gcgttcggcg gtgcgcgaga gcgcgaacag cgccgcgatc agcacgccgc
       61 cggccatggc cgacgcgagc gcgatcagcg tgatgcgcac ggtgaaccac cagctcgcgg
      121 ccagcgaggc gaagttgtcg accagcgtgg tggcgacgag cgagggcgcc ggcagcaggt
      181 acgccgggat gtgcagcgcg cgcactgcgc cttcccatgc gagcaggaac gcggccaccg
      241 acagcgtggg cacgcccacg cgcgcgaacg cgccggcgcg cgtcacgcgt cccctcccgc
      301 gacgttcgcc tgctgcagcg ccgccgacac gcgtgcgcac agcgccgcga actccggcgt
      361 ggcgcgaaag cccgcgtcgc gcggatacgg gacatcgatc gcgatctcct cggcgatgcg
      421 ccccggccgc gccatcacca gcacgcgttc gctgaggaac accgcctcgt agacgctgtg
      481 ggtcacgaag acggccgtgg ccggcgccgc gccgccggcc ggcttgcaca gcgccagcag
      541 cgtctggttc agcgcgaagc gcgtgatctc gtcgagcgcg ccgaacggtt cgtccagcag
      601 ccacagccgc ggcgacgtga ccagcgcgcg cgccagcgac gcgcgcatgc gcatgccgcc
      661 cgagagctcg cgcggcacgg cgttcgccga atcggacagg cccaccgcgc gcagcgccgc
      721 gtcgacgcgc tcgcgcatcg cggccgggcg ttcgccgcgc agcatcagcg gcagttccac
      781 gttgcggcgc acgctggccc agggcagcag cgcggcgtcc tggaacacgt agcccgggat
      841 ctcgtcgccc ggcacgccgc gcgtgatcgt gccggcgctc ggcgcgtcca ggccactgag
      901 caggcgcagc acggtgctct tgccgcaacc cgaggcgccc agcaggctca cgaactcgcc
      961 gggggcgata cgcaggctca cgtcggcgag cgccacgcgg ccgccggaat agacgcgtcg
     1021 gacctgcgcg agggacaggg cagcagaagg gctccgggaa ttcacgcgtc gactgtagct
     1081 tgctccgcgg cgagcgccgc gcaccgtctc cgacggggcc ggcctgcctc acaaatgcgg
     1141 gacatgcatc cggatccgca tccgacttcc gttcgatcca cgcgctgtcg cacctcgcaa
     1201 tccgtcagtc gcgcaggaga caagcaacga gactttgccg gtgccgcgga tccgagggtc
     1261 accctggctg gggtgtcgtg agagaccgga cggcacgact ccaggccgct ggcgactccg
     1321 gtgcgcaagg gcgatggcca cctgccgtgg ccgtcctcga gtgcaaggcg cccgtggtca
     1381 atccaaccac agagagaaca cgagcacaca tgaacaccat ccaccaccgc gctcccatcc
     1441 gcctcatggg tctgtgcgtc gccgtcggcg ccgccttcgc gggcaccgcc catgccgagg
     1501 ccatcaccct gtacggcgtt gccgatgtct acatcgaggc gggcaagggc gaccgccacg
     1561 agatctcgct caactcgggc ggcctctcgg gctcgcgcct gggcttcgac gcgagcaggg
     1621 agctcggcga cgggctcaag gccgtcgcca agctggaagc cggctacgcg ctcgatcacg
     1681 gcacctccac gcagggcggc atcctgttcg gccgccaggc ctatgcgggt ctttccggca
     1741 gcttcggcac ggtgacggcc ggccgccagt acacgcccca gttcctcgcg ctcgatgcga
     1801 acgacccgtt cgacaccggc gccggcagcg cggtgtccag cggcatcgtg agcctgttcg
     1861 cgagccgcgc caacaattcg gtcgtctggt cggcaccgag cagtggtggc ttcgcggtga
     1921 ccgcgatggt ggcgctcggg gaatcgaaca cgggttcgca gaccaactcg accctggcat
     1981 ccgtcgacct gcactacggc agcggcagct cgggcgtcaa cctcaccttc accggggtga
     2041 agaagccgag cgacgacggg gtgaacgcct cggcggtcac gctgggaggc acctatgact
     2101 tcgggtcgct caagctgatg ggcggcatgc aatgggtccg caacgccacc ggcgcccccg
     2161 acacggcgga caaccggcgc gagttcttcg ccggcgtcaa cgtcccgttc ggcgccgacg
     2221 ccctgtggct cggtgccggc accggcaaga cccagggcgt ggccggttcg acggcgagcc
     2281 agtacagcat cggatacgtc cactcggtgg ccaagggcgt cgatgtctac ggcgtgctga
     2341 ccgacatcca gaacggctcg gccaccgcct actccgatga caccgccacc ggcgcaggcc
     2401 ccgcggtgtc ggccggcaag tcggccagcg cggcgcaggt gggcttccgc tatcggttct
     2461 gagtcgccgc gccagccggg gccgccagcc cggggaccga gagcgcgatg atcgacgagg
     2521 gcgtggcgcg cagaccgcaa cctgcgcccg gcccgttttc cctcagctgt ccggcccctc
     2581 accgactgcg ccatgggagt cgctcgtgct atctttcgat accgtgtcga tcgcgtccag
     2641 actgatccct gccctcctgt gtgccgccct ggcggcgccg gccacggcgc aggagtcgat
     2701 cacgctgagc aagattcgcg agtcgggcgt catcaccctg ggctaccggg actcccagtt
     2761 cccgttctcc tacctcgacg atgcgcagcg gcccatcggc tacaccatgg acatctgcga
     2821 ccgcatcgtc gatgcggtgc aggcgcggct ggggctgcgc gacctcgaac ggcgctatgt
     2881 ccaggtgacg tccgccacgc gcattcccat ggtgtccaac ggcacggtcg acctcgaatg
     2941 cggggtcacc accaacacgg cggaacgcga gcgcgatgtc gccttcacga tcaccacctt
     3001 cgtggccgag agccggctgc tctccaagaa gtcctcgccc gtctggcggc tggacgacct
     3061 gcgtggcaag gtcgtcacgt cgacggtcgg cacgaccagc ctggcgcacc tgcaggacct
     3121 gtacgccagg catggcatcg acatgagcat ccttgcgtcg cgggacgacc ccgaggcctt
     3181 ccgcctggtg gccaccgacc gcgcggccgc ctacgcgatg gacgacgcgc tgctgcgcgg
     3241 caccatcgcc cgctccggcc agcccgacga ctacgtcgtg tccaacgagc gcctggcggt
     3301 cgaaccctac ggcatcatgc tggtcaaggg cgaccccgag ttcaagcggc tggccgacca
     3361 ggtgatcatc gggctgttca ggagcggcga gatcctgcag ttgtaccgcc gctggttcca
     3421 gtcgccgacg ccgccgcgcg gcatcaacct ggcctttccg atcttcccgt cgatgctgcg
     3481 actggtggcc aggcccactg attcgccaga cccggcgaac tatccttgaa ctacggccgc
     3541 tcggcgccgc ggtagccgct cacgtcgatg tgcaggcggg cgatcttgtc gtagagcgtc
     3601 ttgcgtccga tgcccagcgc ctcgcaggtc acgctcatgc ggccctgatg gtgcttgagg
     3661 gcccgctcga tgagcacctt ttccacctga tccagctgct gcgcgagcgt cagcacgggc
     3721 gcgtcgccgg tcgtgagcac tgtgtcgtcg gacagcccca gcacgaaacg ctcggcggcg
     3781 ttgcgcaatt cgcgcacgtt gcccggccaa tcatgcgaca tcagctgctg cgacatcgcg
     3841 tcgctgagcg gttcgggttc ccgcgcgtgg cgcgccgagg cttccagcat gaagtgctcg
     3901 aacagcaagg ggacgtcttc gcggcgctcg cgcagcggcg gcagccgcag caccgcgacg
     3961 ttgagccggt aatgcaggtc gctgcggaac agctgctgct cgctcagcag gcgaaggtcg
     4021 gccttcgtcg ccgcgatcac gcgcacgtcg acgggaatct cgtcgttgga acccaggcgc
     4081 tccaccttgc gctcctgcag cacccgcagc agcttgacct ggaagctcat cggcatgctc
     4141 tcgatctcgt ccaggagcag ggtgccgccg tccgcatgct cgaacttgcc gatgcgcttg
     4201 cgcgaggcgt tggtgaatgc gccggcctcg tggccgaaca gttcgctctc gagcagcgac
     4261 tcgggcagtc cgccgcaatt gatcgccacg aagtggcggt cgcgccgcgc gctgtggtcg
     4321 tgcaggcagc gcgcgacgag ctccttgccg gtgcccgtct cgccgatgat catcacgtcg
     4381 cagttggtgg cggcgagctg ctgcacctgg cgctgcagga ccttcatggc gggcgagcgc
     4441 ccgagcagcg tggcatcgat gccccgcctg gcggcaagct gcagccgcaa cgactgcacc
     4501 tccgcgctca gcgctcgcct ttccatggcg cgggtgacga cgtccaccag gcgctcgggc
     4561 gcgaacggct tctcgaggaa gtcataggcg cccgcacgca tggcctcgac ggcgaggtgc
     4621 acgtcgccgt gggccgtgat gaggatgacc gggagggccc ggtcgatctg cagggcgcgc
     4681 ttcatgagcg ccatgccgtc catgttgggc aggcgaacat cggtgaccac cacgccgagg
     4741 aagccggcgc ccagggtggc cagcccttct tcggcagacg cgtgcgccac gacctccaag
     4801 ccggcgagct cgagcgtctg cgcgaggctg cggcgcacgg cgggatcgtc gtcgatcagt
     4861 gcgaccttgt atccatcgaa catgtctatc ctagtgcgac gtcgaattcg aacaccgcgc
     4921 ccgacgtcga cggcaccgca cgcaacgtgc caccgaattc gcgcacgatc tgggaggaga
     4981 tcaccaggcc caggcccagg ccttcgcccg gcgccttggt cgtgaagaac ggctcgaaca
     5041 ggtgctcgtt caattcgtgc gggatgcccg ggccgctgtc gctcacgcgg acgaccgccc
     5101 ggtcggcgct cgccgcggca gcgatggtca gccggcgggc ctcggtcgca cgcatcgcgt
     5161 ccatggcatt ggccatcagg ttcacgaaga cctgctccag gcggtagttg tcgcagcgcg
     5221 cggcgaggcc cggcgggata tccgtgacga gcgcgacgct ctctgcgctg aaccgtgcgg
     5281 cgaggatctg gcgggcgttg tcgatcgcgc ccacgatcgg cagcgatcgc acttcggccg
     5341 gcgccttgcg cgcgacggac ttcagttgcg cggtgatgcg ccccatgcgc tcggccaggc
     5401 cgaggatggc cttgaaattg tcgcgcgcct gcagcgggcg ctcgcgctcc agcaggaggc
     5461 agccgttctg cgcgagcgcg cgcagggccg tcagcggttg gccgagttcg tgcgagatcc
     5521 cggccgacaa ctggcccagc aaggccagct tgccggcctg cacgagctct tcctgcgcct
     5581 ggcgcagcac ctgctccgcg tgctggcgtt cctgtacctc gtggcgcagc gaggcgttgg
     5641 acgattccag ctgcgcggtg cgctgcagca ccttcgtctc gagctcgtcg tgcgcgcgtt
     5701 gcagggccgc gcgcgcagcg accttctgca ccgccacgcg gcggcgttgc agcagcagca
     5761 ggagcagcag cccgagcgac gccgcgatgg cgcccgcgcc ccacgcggca gcgcgtgctt
     5821 cccgccagac gtccctggcg ttggtcagga tgatcaatcg ccatcccatg cgcggcagcg
     5881 gcctctcgtg cacgacctgc atctccatgc ccgtggcctt gagggcgggc acgcgcacca
     5941 gcttcacgtc gctgcccgtc tggccctgcg cctgcgtcaa cgtgctcagc gcctgctgct
     6001 tccatgccgg caccgacgac aggatcacga tgccgcgcga gtcgacgatc agcggcttct
     6061 cgctctcggc gcggaaggcc gaatcgatcc aggtggcctc ggtcggctgc aggctgatcc
     6121 ggacgatcac gacgcccacg gttgccccgt cgcgccggac cgggcgcgcg aagcagactt
     6181 cgggggcgcc gttgtccagg ttcggctcga agcgctgcac cggcagtccg ccgcccaagg
     6241 cctccacgac gcaaggctcg ggcgcggccc tgctcgtgcc gcgcgtgtcg gcgcgatacc
     6301 agttgctgct ggccagcacg tgggccttcg cgtcgaccac gctggcactc aacgcacccg
     6361 agcgggcgac gaagcgcgtc aggcgtcggt tgagcgcgga acgcagggcg gtgttgtcag
     6421 gcgagtcgat cagggccacg gtgtggtcgt cctggtcgag catgcccggc aggtcggcat
     6481 ggttgccaag ctcctggtcc aggactgcgg cgtagaggtc gagctgatgc gcgacgccgt
     6541 cgtccaggcg cacgaacgcg cgcctttccg cccaggcgta agccaggctc gccgagagcg
     6601 ccaccagggc gagggcgccc gacccgatca gaaaaggcgc cgcacgccga atcgccttca
     6661 atcgcttcga atccaatcca gtccccgcaa tggccgccag gcgggcgtcc tgacaagccg
     6721 agctgctcat cttcggccac gagtcgccgt ctggtcaagc cgatgtttcg agccgggtgg
     6781 aatgcgacac acggcccgcc cgcgcaatgt gtggacctcg aaacagtgca ccgtcccatc
     6841 cggccgtgcc gagcggcgcc tgtcagaggc ggcgcgcacc gtcgggcggg ggcaacagcc
     6901 gagctggcac ggcgggtgca ttggaggcgg cggtctttcg atcaggattc ctcaattctt
     6961 ccatttcgaa acatgcacac tcaagctctc cagcgcgtcc ggcgcagtgt caatgacaag
     7021 atcgaccatc gcatcctgca cgtgctgcag aaggacgggc gcatctcgaa tctccagctc
     7081 gcggaacagg tgcacctgtc gccgtccgcg gtgttcgaac gggtcaggcg cctgcagcgc
     7141 gagggctaca tcctgggata ccaggcgcgc ctgagccacg agaagctggg catggaggtg
     7201 gtggccttcg tcgaagtgca gctcgagcgg atgaatccgc aggtcaggca gcacttcgag
     7261 gacgccgtgc gccaacgcgc cgcgatcctg gagtgctaca tgatcgcggg aacgttcgac
     7321 tacctgctca aggtggtcgt ctcggacatg catgcgtgcc gtgaactcgt ggcctccgtg
     7381 atcagcaggc tgccgggcgt gcgcgaggcg cgcacgcatg ccgggatcga acagccgcgg
     7441 ccacccgtgc ctgctccggc cggcgcggag ccgttcatcg acgacgtgga cgccgccctg
     7501 ctgcggctgc tgcaggccga cggccggcgc agcacggcgc gcctggccga ggaactcgac
     7561 ctgacggccg ccgtggtgcg cgagcgcctg tcccggctgt gccgcagggg cttcatcctc
     7621 ggctataccg cggtactcaa cgaggccaag ctgaagtccg gcctgcaggt cttcgcagcg
     7681 gtccgcacgg cggccgcggc cacgggcatc gcgcgtgtct tgagggcggc ggcgctcaac
     7741 catgtcgaga tcctcgaatg ccacgacgtc accggcaact tccagcacct gctgaagctg
     7801 cgcgtggccg acatgcgcag ccacaacgag ttgatggagt cggccgtctg gccgctgccc
     7861 ggcgtgtgcg aggtgcgcac ctatgccgtc atcgacgaga tcaagaacac cacgcggatc
     7921 cccctgtgat gaacaacacc ttcttcccgt gcgccgccat cctggccggc atcggcggcc
     7981 ttctcgccgc ggccggatgc ggccgcgtcc cggacgtggt caagatcggc gtggcccagc
     8041 cgctgtcgaa cgggctcgcg ccgctggggc aggacatggt caacggcgcc cagatggcca
     8101 tcgacgagat caacgcggcc aatggcgtgg acgtgggcgg caaacgcgtg cggctcgcgc
     8161 tggtcacggc cgacgacaag ggcgccaacg aggccggcga cgcggctgcg cgcaagctgg
     8221 tggacgagga cgtcgaggtg gcgatcgccg acctgaactc cggtgtcagc atccatgcag
     8281 cgccgatcta cgcggctgca ggcattccgc agctcgcgat ctccaccaag cccgagttca
     8341 cgcggctggg cctgccgacg acgttgcggc tggttgccag cgacgacctg cagtccaagg
     8401 cgctcggcgc ctatgccgcg cagatcccgg gtgcgtcccg ctatgccgtc gtcgacgacg
     8461 gcacaccgta cggccaaggc ctggcggagg cggccgccaa ggtgatcgag ggcaggggcg
     8521 gccatgtcgg cgtacgccgg accctcgatg acaggaccgt cgatttcggc acgctggtgg
     8581 ccgcgttggc tgccgacaag tcggacctga tcgtgaccac gctcagcgat ttccaggtcg
     8641 acgcgctgat ggcgcagctc gccagggccg ggctgacgca gatgatcatt gtcggcggcg
     8701 acacgatcaa gacggacaag ctggcgcgca aggcgatccc gatcaaggcc gtctacgcga
     8761 cgtcgtcgat cgtcgagccg agggagttcc cgggcgggaa gctctttctc aagaactttc
     8821 gggcgaaatt caaggccgat ccggtctacg gcgcgcacta cgcctacgac gcggtgtacg
     8881 tggtcgccga tgcgctggcc cgcaacagga gcgtcgacaa ggcggcgctg ctgaagacgt
     8941 tgaaggattt cgacggcaac tgtccgatga ccaactcgat gcgcttcggc gccgatggcg
     9001 agcagcgcta cgccgccgtc gcggtctacc agttgcacga cggcgcctgg gacccgctga
     9061 tgcgctcgga tcgttggtga gcgcgacggc ccgggcgcga cgcgcgcgca gcgcgggcgt
     9121 gcccccggcc cgtcggcgcc gcgttcaggc cgcctcggcc cgctcgttcg cgcgccacgc
     9181 ggccaactgg ccggcccact tggcgcgcac cttctcgagg ttgcgctcct tcacatggcc
     9241 gtagccgcgg atctcgtcgg gcagcgcggc cagcgccacg gcgtcggcca ggcggtcgac
     9301 cgtcagcgcg cccagcagct cgtcgatcag ttcccggtac tcggcgatca gcgcgcgctc
     9361 ggtgcggcgc tcggcggtgt agccgaacac gtcgaacgcg gtgccgcgca ggcccttgaa
     9421 cttcgcgagc acgccgaacg cgcgctgcat ccagccgccg taggtcttct tgaccaggtg
     9481 acccttcccg tccttggcgg cgagcagagg gggcgcgagg tggtacttca gcctgaagcc
     9541 gtcctcgaac tggtcggaca gcttggccag gaacgccggg tcggtgtgca ggcgcgcgac
     9601 ctcgtactcg tccttgtagg ccatcagctt gaacaggttg cgcgcgacgg tctcggacag
     9661 ccgcgtgctg gccagcggcg cctcggcggc cttcacgcgg tcgacgatcg tcgcgtacag
     9721 cgcggcgtag tccgcgtcct ggtagccggt gaggaagtcc acgcggcgct gcacggtctc
     9781 caccagcgac ggcttcttga cgaacgagag gacctgcgcc gcggcgaact gcgcctgcac
     9841 ggacgcgagg tcgtgcgcgc agcggcggcc ccattcgaag gcctgcttgt tcttctcgac
     9901 ctgcacgccg ttgagctcga tcgagcgcat cagcgcggcg tggctcagcg gcacgcggcc
     9961 ctgttgccac gcgtagccca gcatcagcgg attggtgaag atcgagtcgc ccagcagcct
    10021 ggtggccacg gtgtcggcgt cgaacgcgcc cagcaggtcc ttgccgaccg cggcctcgat
    10081 ggccgtctcg cacttgccgc ccgggaactg ccagtcggga tcgttgacga agctcgcggt
    10141 gggcgagcgg tgcgtgttca gcgcgacgta ggtgcggcct tcgcgcatca cgcccagcgt
    10201 gaccgggttg gcggcgacga tcgcctcgca accgatcacg aggtcggcct tggcggcgtc
    10261 gaccttggtc gtgaggatgg cgtcctgcgt gtcggcgatc tggatgtggc tccacgtcgc
    10321 gccgcccttc tgcgcgaggc cggccgcgtc ctgcgtgacc acgcccttgc cctccagatg
    10381 cgccgccatg cccagcagct ggccgatggt gatgacgccg gtgccgccga tgccggcgac
    10441 gacgatgccc cacgcggtgc tcgcggcagg cagcgtcggc agcggaatct ccggcagcgc
    10501 cggcgcgtcc ttggccttgg ccggcttcgg cttcttgagc gtgccttcca cggagacgaa
    10561 gctggggcag aagccgttga cgcaggagaa gtcctggttg cacgtgttct ggttgatgcg
    10621 gcgcttgcgg ccgaactcgg tctccagcgg ctcgaccgac aggcagttgc tctgcaccga
    10681 gcagtcgccg cagccctcgc acaccgcctc gttgatgacg acgcggcgcg ggttggcggc
    10741 caacgtgccg cgcttgcgtc ggcggcgctt ctcggtcgcg caggtctggt cgtagaggat
    10801 gaccgtgacg ccgggcacct cacgcagctc gcgctggatc gcgtcgagct ggtcgcgatg
    10861 atgcacggtg acgccgtcgg cgagccgcac gcccgagttg tagcgctcgg gctcgtcgct
    10921 gacgaccacg atgcgcctga cgccctcggc ctccagctcg cgcgtggtcg acgccacgtc
    10981 gatctggccg tcgaccggct ggccgcccgt catcgcgacg gcgccgttga acaggatctt
    11041 gtaggtgatg ttgacgttgg acgacagcga ctgccggatc gccagcaggc ccgagtggta
    11101 gtaggtgccg tcgcccaggt tggcgaagat gtgcttctcg gtggtgaacg gcgcctggcc
    11161 gacccagggc acgccttccc cgcccatctg cgtgaaggtg gacgtcgagc ggtccatcca
    11221 gttgaccatg tagtggcagc cgatgcccgc gaccgcgcgc gagccctcgg gcaccttggt
    11281 cgacgtgttg tgcgggcagc ccgagcagaa ccacggcgtg cggtcgccca cgccgctgga
    11341 cgacccggcc ttgagcgcgg ccagcgcgcg gtccttggcc tcgatgacgg cgatgcgctc
    11401 gtccatgcgg cgcacgatgt cggccggcac gcccagcttc ttcaggcgcc tggccacggc
    11461 cttggcgatg atcgccggcg tcaggtcggc cttggggcgc agcagccagt tgtcgcccgg
    11521 gttcgggttc gaccactcgc cgccggacag gtcgccttcc tgctcgtcga acttgcccag
    11581 caccgtcggg cgcacgtcgt tgcgccagtt gtacagctgt tccttgagct ggtactcgat
    11641 catctggcgc ttttcctcga cgaccaggat ctccttgagg ccccgggcga agtcgcgcgt
    11701 gatcgtggct tccagcggcc acacgacgtt gaccttgtgg atgcggatgc ccagcgagcg
    11761 gcaggtgtcg tcgtccaggc ccaggtcggc cagcgcctgg cgcaggtcgt tgaaggcctt
    11821 gccgctggcg atcaggccga agcggtcgtc cggcgtggac acgacgttgt ggttgagctt
    11881 gttggcgcgt acgtaggcga gtgcggcgta ccacttgaag tccatcagcc gcgcttcctg
    11941 ctccagcggc ggatcgggcc agcggatgtg cacgccgccc ggcggcatca cgaagtcctc
    12001 gggcagcacg atgtcgacgc gatgcgggtc gacgtcgacg gtggccgacg attcgaccac
    12061 c
//
LOCUS       DATCRO010000981         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_258396, whole genome shotgun sequence.
ACCESSION   DATCRO010000981 DATCRO010000000
VERSION     DATCRO010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258396"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VIN75_26835"
     CDS             <1..610
                     /locus_tag="VIN75_26835"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8977860.1"
                     /translation="TRQVVWDEGDQDTPQSRAWWRARNRTYANDHIPRRYATATTDRR
                     DIHEWVYSVLAAPDDTTSLLLIGPTGTGKTHAAYAALRLHCESARHADWLATSAAALL
                     GDLRPAPGRDTEATLNRYLNASLLLLDDLGAAKSSEWVEEVLYRIIDHRYNACLPSIF
                     ATNVSTPDELAARLGERTASRLTEMCRGHLIVLKGADRRRAA"
     gene            607..975
                     /locus_tag="VIN75_26840"
     CDS             607..975
                     /locus_tag="VIN75_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977861.1"
                     /translation="MTTADALLDAPAWRLAARQAAVRNRYQRAMVLRFPDLAKHLCDP
                     PLGLKDPQQWSGYIPGYWEAAGERGHMRPNTSPIRAQLAAILQAAAERSDQQATGAEL
                     GTAELTQRPVAAISPPWSTR"
     gene            972..1283
                     /locus_tag="VIN75_26845"
     CDS             972..1283
                     /locus_tag="VIN75_26845"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8977862.1"
                     /translation="MTALTLPRPGSTLLTDADRDLIRMLADGMTGKQIGRRLGVAPTV
                     VSTRTARLRAKTGTDNTAHLVATATRCGWIGAWELHRIAAQHNAALRAIAQRAADGGA
                     R"
     gene            1280..1678
                     /locus_tag="VIN75_26850"
     CDS             1280..1678
                     /locus_tag="VIN75_26850"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEY8977863.1"
                     /translation="MNATTAPPEVLIDLTLDVAPIPKGRPRVNTQTGRAYTPAATRAY
                     EQRIGWLLRQARVPCAGSGELGVTAVFHLTDGQRADVDNLAKALLDGANGVAYRDDAQ
                     VTCLDARIVRGDGPPRIRLTVYRLTPGETE"
     gene            1675..2214
                     /locus_tag="VIN75_26855"
     CDS             1675..2214
                     /locus_tag="VIN75_26855"
                     /inference="COORDINATES: protein motif:HMM:NF014631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEY8977864.1"
                     /translation="MKRYAPLALTGYAAAIVAANYLTAHHGLIPVGFGLTATAGTYCA
                     GAALMLRNLVQDGLGRRFVVLAIATGAALSALTSPALALASGVAFGVSELVDTALYTP
                     LRRRGWARAVLPASLLGAILDSLLFLAIAGFPVTVPGVAGQLVGKTWAVWLPVAAVTL
                     ARGVRRGAVHGNAVESSGA"
     gene            2207..2869
                     /locus_tag="VIN75_26860"
     CDS             2207..2869
                     /locus_tag="VIN75_26860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977865.1"
                     /translation="MRDAMRAGLLDCMTGPYQGNVLPPEVPWGADNGRFAKDGPRGDW
                     LGHGAWYEWLAGQVDRYGAAFCRFAVAPDVPFSAAGTLAESLPWLAKIRDLGIPAAFA
                     AQNGCDLLGVPWDDLDVLFLAGGPDYPGAPEWKTGEVALHLTWEAHERGKWVHMGRVN
                     SRKRLTIARSFGCDSVDGTYLAFGPDINLMRLRGWLDAAEARPMLTELGRLAADTTET
                     KD"
     gene            2874..3848
                     /locus_tag="VIN75_26865"
     CDS             2874..3848
                     /locus_tag="VIN75_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977866.1"
                     /translation="MTDLPHPDVLEAVAAEIILEHARDVEFMSINEMTAEDERFAGLD
                     EDQRDEACRIISELIPRANVTIDWGDASELERLRTLTDVATFTRLVFADGDKVLARLK
                     RDDWATPEQMERIAEHLAVAFDGYPCIVVPPGIEIEPGPDPAAGLQAAQDASQWHAER
                     LGGGSASHLPFEGELGPQKGSGDVKPRPRHPLRTDEGFGHHLACPWHPDRAGIYATSL
                     GALCRDCWASNFPDAAAGVFAQGTDERTSAARASDVGVTDAASFTDLAAVAARRELLP
                     PPCGHGAMWLIPSGPGVVCGPQPPIAPEDEGIATADGCGLPETNCACG"
     gene            3845..4477
                     /locus_tag="VIN75_26870"
     CDS             3845..4477
                     /locus_tag="VIN75_26870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977867.1"
                     /translation="MTRRLRTDRMAARAREARTTALLRDAYADVRAKVTSGEWSVQRA
                     LAETAPAAFTCPADWDDERVAEWQERWDEFMRDQPQRQRLITLPSRAAIRPGLPRKSA
                     PRGLPRMRKHRRELLINALRPDGTPESAIRVQVLKAYRVLPDDIGLTWAGRVTDGLVS
                     AEQMRADFAADMAADAEIIAAEPSFNLPTGLEFAFEADAADTDPNGEPTE"
BASE COUNT          699 a         1816 c         1448 g          555 t
ORIGIN      
        1 cacccgccaa gtcgtctggg acgaaggcga ccaggacacc ccccaatccc gcgcctggtg
       61 gcgcgcccgc aaccgcacct acgccaacga ccacatcccc cgccgctacg ccaccgccac
      121 aaccgaccgc cgcgacatcc acgaatgggt ctacagcgtc ctagcagccc ccgacgacac
      181 cacgtccctg ctgctgatcg gccccaccgg caccggcaaa acccacgccg cctacgccgc
      241 cctacgcctg cactgcgaat ccgcacgcca cgcggactgg ctcgccacct ccgccgccgc
      301 cctgctcggc gacctgcgcc ccgcccccgg ccgcgacacc gaagccaccc tcaaccggta
      361 cctgaacgcc tcgctgctgc tgctcgacga cctcggcgcc gccaagtcca gcgagtgggt
      421 cgaggaagtg ctgtaccgga tcatcgacca ccggtacaac gcctgcctgc cgagcatctt
      481 cgccaccaac gtctccaccc ccgacgaact ggccgcccgg ctcggcgaac gcaccgcctc
      541 acgcctgacc gagatgtgcc gaggccacct gatcgtgctc aagggcgccg accggcggcg
      601 agcagcatga ccaccgccga cgccctgctc gacgcccccg cgtggcgcct ggccgcccgc
      661 caagccgccg tacgcaaccg ctaccagcgc gccatggtcc tgcgcttccc cgacctcgcc
      721 aagcacctgt gcgacccccc gctcggcctc aaggacccgc agcagtggag cggctacata
      781 cccggctact gggaagccgc cggcgaacgc ggccacatgc gcccgaacac ctcgccgatc
      841 cgcgcccaac tcgccgcgat cctccaagcc gcagccgaac gcagcgacca gcaggcgaca
      901 ggcgccgagc tgggcaccgc cgaactgacg cagaggcccg tggccgccat ctcgccgccc
      961 tggagcaccc gatgaccgcg ctcaccctcc cgcgtcccgg ttcgacgctg ctcaccgacg
     1021 ccgaccgcga cctgatccgc atgctcgccg acggcatgac cggcaaacag atcggccgca
     1081 ggctcggcgt cgcacccacc gtcgtcagca cccgcaccgc ccggctacgc gccaaaaccg
     1141 gcaccgacaa caccgcgcac ctggtggcca ccgcgacccg ctgcggctgg atcggcgcgt
     1201 gggagctgca ccggatcgcc gcgcagcaca acgccgcgct acgagccatc gcccagcggg
     1261 cggcggacgg gggtgcgcgg tgaacgccac cacggcgccg cccgaagtcc tcatcgacct
     1321 cacgctcgac gtcgccccca tccccaaggg ccgcccccgc gtgaacacgc agaccggccg
     1381 tgcgtacacc ccggccgcga cccgcgccta cgagcagcgc atcggctggc tgctgcgcca
     1441 agcccgcgtg ccgtgcgccg ggagcggcga gctcggcgtg accgccgttt tccacctgac
     1501 tgacgggcag cgcgccgacg tcgacaacct cgccaaggcg ctgctcgacg gggccaacgg
     1561 ggtcgcttac cgggatgacg cgcaggtcac gtgcctggac gcccggatcg tgcgcggcga
     1621 cggaccgccg cgcatccgcc tgaccgtgta ccgcctaacc cccggagaga ccgaatgaag
     1681 cgctacgccc ctctcgccct gaccggctac gccgccgcca tcgtcgccgc gaactacctc
     1741 accgcgcacc acggcctgat cccggtcggc ttcggactga ccgccaccgc gggcacctac
     1801 tgcgccggcg ccgcactcat gctgcgcaac ctcgtgcagg acggtctcgg ccgcaggttc
     1861 gtcgtgctgg ccatcgccac gggagccgcc ctgtcggccc tcacgtcgcc cgcgcttgcg
     1921 ctcgcctccg gggtcgcgtt cggcgtcagc gaactagtcg acaccgccct gtacactccg
     1981 ctgcgccgtc gcggctgggc ccgggccgtg ctgcccgcga gcctgctcgg cgcgattctc
     2041 gactccctgc tgttcctcgc catcgccggg ttccccgtca ccgtgcccgg cgtggccggg
     2101 caactggtcg gcaagacgtg ggccgtgtgg ctgccggtcg cggccgtgac gctggcgcgg
     2161 ggggtgcgcc gtggtgcggt acatgggaac gccgtcgagt ccagcggtgc gtgacgcgat
     2221 gcgcgccggg ctgctggact gcatgaccgg cccttatcag ggcaacgtgc tgccccccga
     2281 ggtgccctgg ggtgcggaca acggccgctt cgccaaagac ggcccgcgcg gggactggct
     2341 cggccacggc gcctggtacg agtggctggc cggccaagtg gaccgctacg gcgctgcgtt
     2401 ctgccggttc gccgtcgccc ccgacgtccc gttctccgcc gcgggcaccc tggccgagtc
     2461 cctgccctgg ctcgcgaaga tccgtgacct cggcattccc gccgcgttcg ccgcacagaa
     2521 cggctgcgac ctgctcggcg tgccgtggga cgacctggac gtgctgttcc tggccggcgg
     2581 ccccgactac cccggcgcgc ccgagtggaa gaccggcgag gtcgcactgc acctgacctg
     2641 ggaggcgcac gagcgcggca agtgggtgca catggggcgg gtcaactccc gcaaacgcct
     2701 gaccatcgcg cgcagcttcg gctgcgactc cgtggacggc acctacctgg cgttcggccc
     2761 cgacatcaac ctgatgcggc tgcgcgggtg gctcgacgcc gccgaagcgc gcccgatgct
     2821 caccgaactc ggccgcctag ccgccgacac caccgagacg aaggactgac ccgatgaccg
     2881 acctgccgca ccccgacgtc ctcgaagccg tcgccgccga gatcatcctc gaacacgccc
     2941 gcgacgtgga gttcatgtcc atcaacgaga tgaccgccga agacgagcgg ttcgcggggc
     3001 tcgacgagga ccagcgtgac gaggcctgcc gcatcatcag cgagctgatc ccgagggcga
     3061 acgtcacgat cgactggggc gacgcttccg aactggagcg gctgcgcacc cttacggacg
     3121 tggccacgtt cacgcgcctg gtcttcgccg acggcgacaa ggtgctcgcc cggctcaagc
     3181 gcgatgactg ggcaactccc gagcagatgg aacggatcgc cgagcacctg gcggtggcct
     3241 tcgacggata cccgtgcatc gtcgtgccgc cgggcatcga gatcgagccg ggaccggatc
     3301 cggccgccgg actccaggcc gcgcaggacg cctcacagtg gcacgctgag cgcctgggag
     3361 gtggttcagc gagtcacctg ccgttcgagg gcgagttagg cccgcagaag ggctctggtg
     3421 acgtcaaacc ccggccgcgc catcccctgc ggaccgatga gggcttcggg caccatctcg
     3481 catgcccgtg gcacccggac cgcgccggga tctacgcgac ctcgctaggg gcgctatgcc
     3541 gcgactgctg ggcatccaac ttccccgatg cggctgcggg tgtattcgcc cagggcaccg
     3601 acgagcggac gagcgccgcc cgcgcgtcgg acgtcggcgt gaccgacgcc gcgagcttca
     3661 ccgacctagc cgccgtggct gcacggcgcg aactcctgcc gccgccctgc ggccacggcg
     3721 cgatgtggct cattccctcc gggccgggcg tggtctgcgg gccgcagccg cccatcgcgc
     3781 ccgaggacga gggcatcgcc accgccgacg gctgcggtct ccccgagacg aactgcgcct
     3841 gcggatgacc cgccgtctgc gcaccgaccg gatggccgca cgcgcccgcg aagcccgcac
     3901 cacggcgctg ctacgcgacg cctacgccga cgtgcgcgcc aaggtcacca gcggcgagtg
     3961 gagcgttcag cgggcgctcg ccgagacagc cccggccgcg ttcacatgcc cggccgactg
     4021 ggacgacgag cgggtcgccg aatggcaaga gcgctgggac gaattcatgc gcgaccagcc
     4081 gcagcgccag cgcctcatca cgctgcccag ccgcgccgcc atccggccgg gtcttccccg
     4141 caagtccgcc ccgcgcggtc tgccgcgcat gcgcaagcac cggcgcgaac tgctgatcaa
     4201 cgcgctgcga ccggacggaa cgcccgaaag cgccatacgc gtgcaggtgc tcaaggcgta
     4261 ccgcgtcctg cccgacgaca tcggtctcac ttgggcgggc cgtgtcacgg acggcctggt
     4321 cagcgccgag cagatgcgcg ccgacttcgc ggccgacatg gctgccgacg ccgagatcat
     4381 cgccgccgaa cccagcttca acctgccgac cggccttgag ttcgcattcg aggccgacgc
     4441 cgccgacacc gacccgaacg gagagccgac cgaatgaccg gccaccacgc aggacaccac
     4501 ccccgcacag ccgctccg
//
LOCUS       DATCRO010000982         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_528821, whole genome shotgun sequence.
ACCESSION   DATCRO010000982 DATCRO010000000
VERSION     DATCRO010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528821"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1293
                     /gene="lplT"
                     /locus_tag="VIN75_26875"
     CDS             16..1293
                     /gene="lplT"
                     /locus_tag="VIN75_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY8977868.1"
                     /translation="MKRGFYTIMSAQFFSSLADNALFVAAVEMLRTAGAPAWQRTALV
                     PMFALFYVVLAPFLGAFSDAVPKGRVMFYGNALKVVACLMMLYGSHPLIAYAFVGLGA
                     AAYSPAKYGILTELLPTSQMVKANGWIEGLTILSIILGVGLGGQLIGPRLSPYLLSVH
                     VPFIDFGVRTPAQATVAFMVLLYVIAAAFNLRIPRTDAVLEPFTGRVAELVRDFSQCN
                     ARLWDDKLGQISLATTTLFWGASANLRYLVLAWAGAALAYDTPRATVLLLLVGLGTAA
                     GAVVASITVRLVSATNVILLGAALGAAVIAINGMHSVWVAGPFLIVLGAIGGFMVVPM
                     NALLQHRGHNLMGSGRSIAVQNFNEQAAILGLGAFYSALTRGGLSVFVSIAVFGGLVA
                     FASWLIWRWHKRNLVEHREEVEGLLAIARSDNH"
     gene            1310..2209
                     /locus_tag="VIN75_26880"
     CDS             1310..2209
                     /locus_tag="VIN75_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8977869.1"
                     /translation="MTSIHPRRGSAGPVLALLANAFTWGVSWWPFRQLQAMGLHPLWA
                     TVVIYVLSALAILAWRPSALRQFAQHPALWLIFVGSGVTNATFNWSVSIGDVVRVVLL
                     FYLMPLWSLLLARVILKETLTPSAIARVAMALAGAALVLSQGGAPGGGGGHAGLLPDV
                     LALLGGFAFALNNVMLKREAAQPEEGRALAMFLGGAVVAGATATALSAAASAGVQWPV
                     ASAAWVLPALGLSLAFMASNLCLQYGAARLSAAATAVVMPCEVLFAALTSVWWGGATL
                     HASVLAGGALILAATLASILEKR"
     gene            2294..2785
                     /locus_tag="VIN75_26885"
     CDS             2294..2785
                     /locus_tag="VIN75_26885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8977870.1"
                     /translation="MAAKTLYDFSANSIQGKPADFGSQRGKVFLIVNTASKCGFTPQF
                     AGLEQLASDYKDRGLVVVGFPCNQFGHQDPGANAEIESFCQLNYGVSFPMMEKVDVNG
                     AKAHPLWKWLKSEKPGVLGTEAIKWNFTKFLVGKDGHVVKRFAPNDEPDKLRADIEAA
                     LAA"
     gene            complement(2787..>3336)
                     /locus_tag="VIN75_26890"
     CDS             complement(2787..>3336)
                     /locus_tag="VIN75_26890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8977871.1"
                     /translation="GDATPALFGVLAGCIESRFSNAQGQTSVVENVEAGRTFGLASFV
                     TGLPSTYEALAREPTRLLVIGPPAYAWLMDEVPGFARALMREFARRYDGTLKLLEAAR
                     HRGAEERLCLALQHLRREGRATPVGAGPWTMQLTQSELATRANVSRQTANEWLAHWAA
                     RGWVARRYRALEIARWPTPADA"
BASE COUNT          421 a         1174 c         1180 g          561 t
ORIGIN      
        1 gacgatgccg tacgaatgaa aagaggtttc tacaccatca tgtcggcgca gttcttcagc
       61 tcgctggcgg acaacgcatt gttcgttgcc gccgtcgaga tgctgaggac tgcgggcgcg
      121 ccggcgtggc agcggacggc gctcgtgccc atgttcgcgc tgttctacgt cgtcctggcg
      181 ccctttctcg gcgcgttctc cgacgcggtg cccaagggcc gggtgatgtt ctacggcaac
      241 gcgctgaagg tggtcgcgtg cctcatgatg ctttacggca gccacccgct gatcgcgtac
      301 gcgttcgtcg gcctgggcgc ggcggcgtac tcgcccgcca agtacggcat cctcaccgag
      361 ctgctgccca cctcgcagat ggtcaaggcc aacggctgga tcgagggcct gaccatcctg
      421 tccatcatcc tgggcgtggg cctgggcggc cagctgatcg ggccgcggct gtcgccctac
      481 ctgctgtcgg tgcacgtccc cttcatcgac ttcggcgtgc gcacgccggc gcaggccacc
      541 gtggcgttca tggtgctgct gtacgtgatc gccgcggcgt tcaacctgcg catcccgcgc
      601 accgacgccg tgctggagcc gttcacgggc cgcgtcgccg agctggtgcg cgacttcagc
      661 cagtgcaacg cgcgcctgtg ggacgacaag ctgggccaga tctcgctggc gaccacgacg
      721 ctgttctggg gcgcgtcggc caacctgcgc tacctggtgc tcgcctgggc gggcgcggcg
      781 ctcgcctacg acacgccgcg cgccaccgtg ctgctgctgc tggtggggct gggcaccgcc
      841 gccggcgcgg tggtcgcctc catcacggtg cgcctggtga gcgccaccaa cgtgatcctg
      901 ctgggcgccg cgctcggtgc ggccgtcatc gccatcaacg gcatgcacag cgtgtgggtc
      961 gccgggccgt tcctgatcgt gctgggcgcg atcggcggct tcatggtcgt gccgatgaac
     1021 gcgctgctgc agcaccgcgg ccacaacctg atgggctcgg gccgttcgat cgcggtgcag
     1081 aacttcaacg agcaggccgc catcctgggc ctgggcgcgt tctattcggc gctgacgcgc
     1141 ggcggcctgt cggtgttcgt ctcgatcgcc gtcttcggcg ggctggtggc cttcgccagc
     1201 tggctgatct ggcgctggca caagcgcaac ctcgtcgagc accgcgagga ggtcgagggc
     1261 ctgctcgcga tcgcgcgctc cgacaaccac tgagccgcgc gatcgcacca tgacgtcgat
     1321 ccacccgcgg cgtggctccg cgggcccggt gctcgcgctg ctggccaacg ccttcacctg
     1381 gggcgtgtcg tggtggccgt tccgccagct ccaggcgatg ggcctgcatc cgctgtgggc
     1441 cacggtcgtc atctacgtgc tgtcggcgct ggccatcctg gcctggcggc cgtccgcgct
     1501 gcgccagttc gcgcagcatc cggcgctgtg gctcatcttc gtcggctcgg gggtcaccaa
     1561 cgccacgttc aactggtcgg tgagcatcgg cgacgtcgtg cgcgtggtgc tgctgttcta
     1621 cctgatgccg ctgtggtcgc tgctgctggc gcgcgtcatc ctcaaggaga cgctgacgcc
     1681 gagcgcgatc gcgcgcgtgg cgatggcgct ggccggcgcg gccctcgtgc tgtcgcaggg
     1741 tggcgcgcct ggcggcggcg gcggccatgc cggactgctg cccgacgtgc tggcgctgct
     1801 gggtggcttc gcgttcgcgc tgaacaacgt gatgctcaag cgcgaggcgg cgcagcccga
     1861 ggaagggcgc gcgctggcga tgttcctggg cggcgcggtg gtggccgggg ccacggcgac
     1921 ggccctgtcg gccgcggcgt cggcgggcgt gcagtggccg gtggcgtcgg ccgcctgggt
     1981 gctgcccgcg ctcggcctgt cgctcgcgtt catggcctcc aacctgtgcc tgcagtacgg
     2041 ggctgcgcgg ctgtcggccg cggccactgc ggtggtgatg ccgtgcgagg tgctgttcgc
     2101 cgcgctgacc tccgtctggt ggggcggcgc cacgctgcac gccagcgtgc tggcgggcgg
     2161 tgcgctgatc ctggctgcca cgctggccag catcctcgag aagcgctgaa tccgctgttg
     2221 tttcgcgttc acgcgcgtgc cgcggcggcg cgcgccgatc taccatcccg atcctcagct
     2281 tccaggagag cccatggccg ccaagacgct ttacgacttt tccgcgaatt ccatccaggg
     2341 caagccggcc gacttcggct cgcagcgcgg caaggtcttc ctgatcgtca acaccgccag
     2401 caagtgcggc ttcactccgc aattcgcggg cctggagcag ctggcctccg actacaagga
     2461 tcgcggcctg gtcgtcgtcg gcttcccgtg caaccagttc ggccaccagg accccggcgc
     2521 gaacgccgag atcgagagct tctgccagct caactacggc gtcagcttcc cgatgatgga
     2581 gaaggtggac gtcaacggcg ccaaggcgca tccgctgtgg aagtggctga agtcggagaa
     2641 gcccggcgtg ctgggcaccg aggcgatcaa gtggaacttc accaagttcc tggtcggcaa
     2701 ggacggccac gtcgtcaagc gcttcgcgcc gaacgacgag ccggacaagc tgcgcgcgga
     2761 catcgaggcc gcgctcgcgg cctgagtcag gcgtccgcgg gcgtcggcca gcgcgcgatc
     2821 tccagcgccc ggtagcgccg cgccacccac ccgcgcgcgg cccagtgcgc cagccattcg
     2881 ttggccgtct ggcgcgacac gttggcccgc gtggccagtt ccgattgcgt gagctgcatc
     2941 gtccacgggc cggcgcccac cggcgtggcg cgaccctcgc ggcgcaggtg ctgcagcgcc
     3001 aggcacagcc gctcctcggc gccgcggtgg cgcgcggcct ccagcagctt cagcgtgccg
     3061 tcgtagcggc gcgcgaactc gcgcatcagc gcccgcgcga agccgggcac ctcgtccatg
     3121 agccacgcat aggccggcgg gccgatcacc agcagccgcg tgggctcccg cgcgagcgcc
     3181 tcgtaggtgg aaggcaggcc cgtcacgaac gacgcgagtc cgaaggtgcg tccggcctcg
     3241 acgttctcca ccaccgatgt ctgcccctgc gcattggaga agcgcgattc gatgcatccg
     3301 gccagcacgc cgaacagagc gggcgtggcg tcgccc
//
LOCUS       DATCRO010000983         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_371255, whole genome shotgun sequence.
ACCESSION   DATCRO010000983 DATCRO010000000
VERSION     DATCRO010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371255"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..617
                     /locus_tag="VIN75_26895"
     CDS             39..617
                     /locus_tag="VIN75_26895"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEY8977872.1"
                     /translation="MKLNTTLVLIALAFAGGTASAATVGQFTTLPDLTMSANPTVGPT
                     GAAVHGNFDATQDIDYVYNFNIADVSDLWTYAKEFESLDVSMNPATFTLYTGTSDGTA
                     ATAGTMVGSTFSFAGGDAVTTTYTNLAAGNYYFEVMGSATGVLGADYDLAIQANAPGT
                     PLPSVPEPANMALLAAGLGLMGFMAKRRSRQQ"
     gene            complement(693..1154)
                     /gene="aroQ"
                     /locus_tag="VIN75_26900"
     CDS             complement(693..1154)
                     /gene="aroQ"
                     /locus_tag="VIN75_26900"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HEY8977873.1"
                     /translation="MESLRTLIDARLLLATDVAQYKWNHHLPIEDPARETQVIDALGK
                     RADALGVPQPWAEKFFRAQIEASKTAQNELFQGWDVFRRGEFSGAPDLASVTRPKLDK
                     LTDELLHAIAENWTVLADPKRRDDVARAMHPMQAEDLSAKAVAQAIAPLAP"
     gene            complement(1158..1934)
                     /locus_tag="VIN75_26905"
                     /pseudo
     CDS             complement(1158..1934)
                     /locus_tag="VIN75_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            2246..>4087
                     /gene="katG"
                     /locus_tag="VIN75_26910"
     CDS             2246..>4087
                     /gene="katG"
                     /locus_tag="VIN75_26910"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY8977874.1"
                     /translation="MSDQAKCPFPHDVSQHAVASAPRNADWWPNQLNLDILHQQSSKS
                     DPMGQGFDYTEAFKQLDLDAVVADLRALMTDSQDWWPADYGHYGPLFIRMAWHAAGTY
                     RITDGRGGAGTGAQRFAPLNSWPDNGNLDKARRLLWPIKQKYGRNLSWADLIVLTGNV
                     ALESMGFKTFGFAGGRPDIWEPHLEVYWGPEKKWLGDERYGSNRELAHPLAAVQMGLI
                     YVNPEGPNGNPDPVKSGFDIRETFARMAMDDEETVALVAGGHTFGKAHGAGPAANVGA
                     EPEGASIEEQGLGWANSFGSGKGLHTITSGIEGAWTPTPTKWDMSFFETLFKYEWELT
                     RSPAGAHQWKPVGASGDGTVPDAHDPSKRHAPMMTTADMALRTDPAYEKISRRFMADP
                     AAFADAFARAWFKLTHRDMGPRARYLGKLVPKEELIWQDPIPAVDHPLVDDADVAALK
                     QAVLASGLSAAELVGTAWASASTFRGGDKRGGANGARIRLAPQKDWAVNQPAQLAKVL
                     ESLERIQRDFNGAQKGGKKVSLADLIVLAGNAAVEDAARKAGQAITVPFAPGRADASQ
                     AQTDVDSFKVLEPVADGFRNYARKGFEAAAAELLVDKANLLTLTAPEM"
BASE COUNT          660 a         1416 c         1411 g          600 t
ORIGIN      
        1 ctcggcctta tccccctcac cgctaggaga agatttttat gaaactgaac accaccctcg
       61 tcctgattgc cctggctttc gccggtggca ccgcctccgc tgccacggtc ggacagttca
      121 ccacgctgcc tgacctgacc atgtcggcca acccgacggt cggcccgacc ggcgcggccg
      181 tgcacggcaa cttcgacgcg acgcaggaca tcgactacgt ctacaacttc aacatcgccg
      241 acgtgtccga cctctggacg tacgccaagg agttcgagtc gctggacgtg tcgatgaacc
      301 cggcgacctt cacgctgtac accggtacct cggacggcac cgccgcgacc gcgggcacca
      361 tggtgggttc gacgttctcg ttcgccggcg gtgacgccgt cacgacgacg tacaccaacc
      421 tggccgccgg caactactac ttcgaagtga tgggctcggc cacgggcgtc ctgggcgccg
      481 actatgacct ggccatccag gccaacgccc ccggcacgcc gctgccgtcg gtgccggaac
      541 ccgccaacat ggcgctcctg gccgccggcc tgggcctgat gggcttcatg gccaagcgtc
      601 gctcgcgcca gcagtaagcc gacgattcac cggcgccgca cggcgccggc ctgatggaaa
      661 aggcagcctc ggctgccttt ttcttcgccc gttcagggcg ccagcggcgc gatcgcctgg
      721 gcgacggcct tcgcgctgag atcctcggcc tgcatcgggt gcatggcgcg ggcgacgtcg
      781 tcgcgccgct tcgggtcggc cagcaccgtc cagttctcgg cgatcgcgtg cagcagctcg
      841 tcggtgagct tgtcgagctt ggggcgcgtg accgaggcga ggtcgggggc gccggagaac
      901 tcgccgcggc ggaacacgtc ccagccctgg aacagctcgt tctgcgccgt cttggacgcc
      961 tcgatctgcg cgcggaagaa cttctcggcc catggctggg gcacgcccag ggcgtcggcg
     1021 cgcttgccga gcgcgtcgat cacctgcgtc tcgcgcgccg ggtcctcgat gggcaggtgg
     1081 tggttccact tgtactgcgc cacgtcggtg gccagcagca ggcgggcgtc gatcagcgtg
     1141 cgcagcgact cgatgcccca tgggtacgcg agccactcct cgttgagccg cgcgtacgtg
     1201 ccgctctcga tggcctggtg cagccactgg tcgacgtagg ccttgagcgc gaggtcgcgt
     1261 ggcagcagga aggccttctc ggagaagtcg aacggcgtgt ccgggtggat cgcgcacagt
     1321 ttcggatgca ggcgctgctg gagccgcgcc tcgatggcgt cggtgatcat cacgtcggcc
     1381 tcgcctgcca ggatgcggtc gaagatggtg acgttgtcgg gatggaccgt gagggtcgcg
     1441 tgcggcgcgt gcgcgcgcgc gaagcgctcg ttggtgccgc ccgggttgac gatcagccgc
     1501 acctcggggc ggtcgatgtc ggccagcgtc tggtacttgc gggcatcgtc gcagcgcgcg
     1561 atcggcgtct tgccgtcgcg catcgtcggc agcgagaaga acgccttctt ctggcgttcc
     1621 agcgagacgg acacgccgcc cacggcgatg tcgaagcggt cgtcggccag gtcgcccatg
     1681 agcgcgctcc aggtggtggg cacgacctgc atcctgacgc ccagcgagcg cgccaggtcc
     1741 tcggccagcg cgatgtccag gccgatgaag cggtcgccgc cggccacgcg gtaggtgaac
     1801 ggcttgtagt cgccggtgct gcccacgcgc agcgtgccgc gcgccaggat ctcgtcgagg
     1861 cggctggacg acgccgcggc cgtccggggg gcgacggccg catcggccgc gaaggtggcg
     1921 gccggggctg cgatggcgat gacggcgagc gcggcaatcc aggtggacag gcgggacttg
     1981 gcggacacgg gagactccgg gtggcgacga cggttcggga gtctaggcaa tcgaggccgc
     2041 cccgcgactc cttgctcgac cagggcggca tgaatgtctc accgggacaa ggcaatggcc
     2101 gggcaggata cgaaccgtaa aaactatcgg tccattaata tttattgatt tgctcaattt
     2161 actcgcagcg gagacagtcc aggcatgacg cgacgcgacc tcggccgccg cgccttcccg
     2221 atcacacgat tcaaggagat ccagcatgtc cgaccaagcg aagtgcccgt tcccgcacga
     2281 tgtcagccag cacgccgtcg cgagcgcccc gaggaacgcc gactggtggc cgaaccagct
     2341 caacctcgac atcctgcacc agcagtcgag caagtccgac ccgatgggcc agggcttcga
     2401 ttacaccgag gcgttcaagc agctcgacct cgacgcagtg gtcgccgacc tgcgcgcgct
     2461 catgaccgac tcccaggact ggtggcccgc cgactacggc cactacggcc cgctgttcat
     2521 ccgcatggcc tggcatgcgg ccggcaccta tcgcatcacc gacggccgcg gcggcgccgg
     2581 caccggcgcg cagcgcttcg cgccgctcaa cagctggccg gacaacggca acctggacaa
     2641 ggcccggcgc ctgttgtggc cgatcaagca gaagtacggc cggaacctgt cctgggccga
     2701 cctcatcgtc ctgaccggca acgtcgcgct cgaatccatg ggcttcaaga ccttcggctt
     2761 cgccggcggc cgccccgaca tctgggagcc gcacctcgag gtgtactggg gtccggagaa
     2821 gaagtggctg ggcgacgagc gctacggctc gaaccgcgag ctggcgcatc cgctggcggc
     2881 ggtgcagatg ggcctgatct acgtgaatcc ggaaggtccg aacgggaacc ccgacccggt
     2941 gaagtccggc ttcgacatcc gcgagacctt cgcgcgcatg gcgatggacg acgaggagac
     3001 cgtcgccctg gtggccggcg gccacacctt cggcaaggcg cacggcgccg ggccggccgc
     3061 gaacgtcggc gcggagcccg agggcgccag catcgaggag cagggcctgg gctgggccaa
     3121 cagcttcggc tcgggcaagg gcctgcacac gatcaccagc gggatcgagg gcgcgtggac
     3181 gcccacgccg accaagtggg acatgagctt cttcgagacg ctgttcaagt acgaatggga
     3241 gctcaccagg agcccggccg gcgcgcacca gtggaagccg gtcggcgcga gcggcgacgg
     3301 cacggtgccc gatgcgcacg acccttcgaa gcgccacgcg ccgatgatga ccaccgccga
     3361 catggcgctg cgcaccgacc cggcctacga gaagatctcg cgccgcttca tggccgaccc
     3421 cgcggcgttc gcagatgcgt tcgcccgcgc ctggttcaag ctgacgcacc gcgacatggg
     3481 cccgcgggcc cgctacctcg gcaagctggt gcccaaggag gagctgatct ggcaggaccc
     3541 gatccccgcg gtcgaccatc cgctcgtcga tgacgccgac gtcgccgcgc tgaagcaggc
     3601 ggtactggcg tccggcctga gcgcggcgga gctggtgggc acggcctggg cttcggcctc
     3661 gaccttccgc ggcggcgaca agcgcggcgg cgccaacggc gcgcgcatcc gcctcgcgcc
     3721 gcagaaggac tgggccgtca accagccggc gcagctggcc aaggtgctcg aatcgctcga
     3781 acgcatccag cgcgacttca atggcgcgca gaagggcggc aagaaggtct cgctggccga
     3841 cctgatcgtg ctggcgggca acgccgccgt cgaggacgcc gccaggaagg ccggtcaggc
     3901 gatcacggtg ccgttcgcgc ccggccgcgc ggatgcgtcg caggcgcaga ccgacgtcga
     3961 ctcgttcaag gtgctcgaac ccgtcgccga cggcttccgc aactacgcac gcaagggctt
     4021 cgaagcggca gcggccgaac tgctcgtcga caaggcgaac ctgctcacgc tcaccgcgcc
     4081 cgagatg
//
LOCUS       DATCRO010000984         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_764759, whole genome shotgun sequence.
ACCESSION   DATCRO010000984 DATCRO010000000
VERSION     DATCRO010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764759"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VIN75_26915"
     CDS             <1..249
                     /locus_tag="VIN75_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8977875.1"
                     /translation="EPLTGRRHQIRRHFKHLAHPLVGDTTHGKGAHNRAVAAWLGTSR
                     LWLHAIRLELPAVDGRPPLVIESAPGAEWRTLLPAAGR"
     gene            complement(259..1254)
                     /locus_tag="VIN75_26920"
     CDS             complement(259..1254)
                     /locus_tag="VIN75_26920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8977876.1"
                     /translation="MTSMLKAQYTQRGPVPQDVIAPVPFDRPVLAAGQALVAVLAAPI
                     NPSDVLMLTGQYGMLPPLPAVGGSEGVGRVVELGPDSQGPGVGQTVLLPPGAGTWATH
                     VVVPANRLIPLPADADVRQLAMLTVNPPTASLLLSDFADLQPGDWIIQNGANSGVGSY
                     VVQLARLRGFKTVNIVRRDSAVAGVSAQGGDVVLVDGDDLAERVKAATSGAAIKLGID
                     AVGGKATMRLAQTLADGASIVNYGALSGEPCIVTPRELIFKDVTLKGFWLARWFRVAP
                     QARQFALLTELAGLIAAGKLSAPIQATYDVSQIKEAVAAAAAGERQGKILITPRH"
     gene            1388..2293
                     /locus_tag="VIN75_26925"
     CDS             1388..2293
                     /locus_tag="VIN75_26925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8977877.1"
                     /translation="MDKLRALQYLVKVADTLSFSKSARAFGVPASSISRRITDLEADL
                     GVQLLHRTTRTVRLTEIGARYLEQVRAGMAQLADADELVGQSSATPSGTLRISAMPGY
                     GALLLMPALQDFSERFPDIVLDVHLSDALVDLGRDQIDIAIRGGREPQDRVVARKLDP
                     NRFVLAASPGYLARMGTPRTLADLQGHRALMYRGPNAVIRWQGLDEDGWHEVPVSPAF
                     ISNDGAALIAMACRHRGIVLLSEWGLRSALDRGELVQVTLDRPVSVGRGGEAGIYLLY
                     LQTRYQIPKVRVAVEFLVDRLGSVA"
     gene            complement(2334..3368)
                     /locus_tag="VIN75_26930"
     CDS             complement(2334..3368)
                     /locus_tag="VIN75_26930"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8977878.1"
                     /translation="MNKRAGTAPTIIDVARHAGVSIKTVSRVMNKEPTVHADTRARVQ
                     EAVAALNYRPQLSARSLAGARSFLIGLLYYDPSAAFVAGVQQGATLGCREAGRHLVVE
                     SLRKDDGHMHAQIEAMLAALRPDGMILTPPLCDDPEILDVLRANRTPCVLISPGTEGH
                     GMAAVRMDDALAAEELTNLLISLGHKDIAFIEGNQSASERRRAGYERALKAHRIPVDR
                     HLIMPGNFQFEAGVAAAQALFAGAKPPTAIFAANDDMALGALTAAQRLGIAVPHDVAI
                     VGFDDSRAATLVWPELTTVRQPLAGMAMAAVEMLLTGVARPGDAAPVRVLPHEVVVRG
                     STAAPAKGRA"
     gene            3669..4214
                     /locus_tag="VIN75_26935"
     CDS             3669..4214
                     /locus_tag="VIN75_26935"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY8977879.1"
                     /translation="MKKTWLKRTLLGVAVSTALLGSVAAYSQGSGFHHGPPTPEQIAQ
                     HEQHMLEHVGKKLNLDANQSAKLKALADLAIADHAPPAAGTDPHAKMAALISGSTFDR
                     AGAQALANQHVAKMQADAPGLINAFGDFYDSLNATQQAQVRELAAHHHGFFMMGMGFG
                     GHEGPHGAHGMHGDASGATGG"
     gene            4314..4994
                     /locus_tag="VIN75_26940"
     CDS             4314..4994
                     /locus_tag="VIN75_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8977880.1"
                     /translation="MRRLLMIDDDALLAAPLATYFRRFDYDLDSATTPSEGLAKLRAA
                     PYDAAILDVMLPEMDGFELCRTIRRESDIPIVMLTARGDVMDRIVGLEIGADDYIPKP
                     FEPRELLTRIQTILRRARAPAAAPQGRLVFDGLEIDLERRQVVRQGEPVELTGTEFEL
                     LALLAKAPQKVFHRDEILNRLRGQDADLYTRAVDILVSRLRRKLEPLDCIKTLRNAGY
                     AFAGGHVA"
BASE COUNT          738 a         1833 c         1780 g          716 t
ORIGIN      
        1 gagccgctga ccggccggcg ccaccagatc cgccgccact tcaagcacct cgcgcatccg
       61 ctggtgggcg acaccacgca cggcaagggc gcgcacaacc gcgcggtcgc ggcgtggctg
      121 ggcacgtcgc ggttgtggct gcatgcgatc cgactggagc tacccgcggt cgacgggcgg
      181 ccaccgctgg tgatcgaatc ggcgccggga gcggagtggc gcacgctgct cccggcggcg
      241 ggccgctgac acctcgtgtc agtgccgggg cgtgatcagg atcttgccct ggcgctcgcc
      301 ggcggccgcg gctgccaccg cctccttgat ctgcgacacg tcgtaggtcg cctggatggg
      361 cgcgctgagc ttgcccgcgg cgatcaggcc cgccagctcg gtcagcagcg cgaactgccg
      421 ggcctggggg gcgacgcgga accagcgcgc cagccagaag cccttgagcg tcacgtcctt
      481 gaagatcagc tcgcgcggcg tcacgatgca cggctcgccg ctcagcgcgc cgtagttgac
      541 gatgctcgcg ccgtcggcca gcgtctgcgc caggcgcatg gtggccttgc cgccgacggc
      601 gtcgatgccc agcttgatgg ccgcgccgct ggtggccgcc ttgacgcgtt cggccaggtc
      661 gtcgccgtcc accagcacca cgtcgccgcc ctgggcggac acgcccgcga cggcggagtc
      721 gcgacgcacg atgttcacgg tcttgaagcc gcgcagcctg gccagctgca ccacgtagct
      781 gcccacgccc gaattggcgc cgttctggat gatccagtcg ccgggctgca ggtcggcgaa
      841 gtcgctcagg agcagcgagg cggtgggcgg gttgaccgtc agcatggcca gctggcgcac
      901 gtcggcgtcg gccggcaacg ggatgaggcg attggccggc accacgacgt gcgtggccca
      961 cgtgcccgcg ccgggcggca gcagcacggt ctggccgacg cccggcccct ggctgtccgg
     1021 gccgagttcg acgacacgtc ccacgccttc gctgccgccc acggcgggca gcggcgggag
     1081 catgccgtac tggccggtca gcatcagcac gtcgctcgga ttgatgggcg cggccagcac
     1141 ggccaccagg gcctggccgg cggccagcac ggggcggtcg aacggaacgg gggcgatcac
     1201 gtcctgcggc accgggccgc gttgcgtgta ctgggctttg agcatgctgg tcatgatggt
     1261 gtccgggtgc cgttgccggc gtcttgtgtt gcgatgggct gaatcgtaga gagccgcccg
     1321 gttttgcgga atacgttgca ttcgctaaaa tcctttgcct gaatgacaac aatgtggcca
     1381 ccgggacatg gacaagctgc gtgcgctgca atacctggtc aaggtggccg acaccctgag
     1441 cttctcgaag tccgcgcgcg ccttcggcgt gccggcctcg tccatctcgc gccgcatcac
     1501 cgacctggag gccgacctgg gtgtgcagct cctgcatcgc acgacccgaa ccgtgcgcct
     1561 gaccgagatc ggcgcgcgct acctcgagca ggtgcgcgcc ggcatggccc agctcgctga
     1621 cgccgacgaa ctggtgggcc agagcagcgc cacgccgagc ggcacgctgc gcatcagcgc
     1681 catgcccggc tacggcgcgc tgctgctgat gcccgccctg caggacttca gcgagcgctt
     1741 ccccgacatc gtgctggacg tgcacctgag cgacgcgctc gtcgacctgg gccgcgacca
     1801 gatcgacatc gccatccgcg gcggccgcga gccgcaggac cgcgtggtgg cgcgcaagct
     1861 cgacccgaac cgcttcgtcc tcgccgcctc gcccgggtac ctcgcgcgca tgggcacgcc
     1921 gcgcacgctg gccgacctgc aagggcaccg cgcgctgatg taccgcgggc ccaacgcggt
     1981 catccggtgg cagggcctgg acgaggacgg ctggcacgag gtgcccgtct cgccggcctt
     2041 catcagcaac gacggggccg ccctgatcgc catggcctgc cggcatcgcg gcatcgtcct
     2101 cctgtcggag tgggggctca ggagcgcgct cgatcgcggc gaactcgtcc aggtcacgct
     2161 ggaccggccg gtgtcggtgg ggcgcggggg cgaagcgggc atctacctgc tgtacctgca
     2221 gacgcgctac cagatcccca aggtgcgcgt cgcggtggaa ttcctggtcg atcgattggg
     2281 cagcgtcgcg tgaacgcgtg gcacggcgaa cgccggccgc agcgccggga cgctcaagcg
     2341 cggcccttgg cgggcgcggc ggtcgacccg cgcaccacca cctcgtgcgg cagcacgcgc
     2401 accggcgcgg cgtcgcccgg cctggccacg ccggtcagca gcatctcgac cgccgccatc
     2461 gccatcccgg ccagcggctg gcgcaccgtc gtcagctcgg gccagaccag cgtggccgcg
     2521 cgcgaatcgt cgaagccgac gatggcgacg tcgtgcggca ccgcgatgcc caggcgctgc
     2581 gccgcggtga gcgcgcccag cgccatgtcg tcgttggccg cgaagatggc cgtgggcggc
     2641 ttcgcgccgg cgaacagcgc ctgcgccgcg gcgacgccgg cctcgaactg gaagttgccc
     2701 ggcatgatca ggtggcggtc gacggggatg cggtgcgcct tgagcgcgcg ctcgtatccc
     2761 gcgcgccggc gctcgctggc cgactggttg ccctcgatga acgcgatgtc cttgtggccc
     2821 aggctgatca gcaggttggt gagctcctcg gcggccagcg catcgtccat gcgcacggcg
     2881 gccatgccgt ggccctcggt gccgggcgag atgagcacgc acggcgtgcg gttggcgcgc
     2941 agcacgtcca ggatctcggg atcgtcgcac agcggcggcg tcaggatcat gccgtcgggc
     3001 cgcagcgcgg cgagcatggc ctcgatctgc gcgtgcatgt ggccgtcgtc cttgcgcagc
     3061 gactcgacga ccaggtggcg gcccgcctcg cggcaaccga gcgtggcgcc ctgctgcacg
     3121 ccggccacga acgcggcgct cgggtcgtag tacagcaggc cgatcaggaa cgacctggcg
     3181 ccggcgaggc tgcgcgcgga cagctgcggc cggtagttca gcgccgcgac ggcctcctgc
     3241 acgcgggccc gcgtgtccgc atggaccgtg ggctccttgt tcatcacgcg cgagacggtc
     3301 ttgatcgaca cccccgcgtg acgggccacg tcgatgatgg tgggggctgt gcccgctcgc
     3361 ttgttcactg tgtcgccact ttcggttcgt tctctcggcg gccggggatt gtgtcgaccc
     3421 ggcgcgaaac gcgtcgtttt cgccagtcta atgtttcccg tttgaaaatc cgggccgaac
     3481 catgggattt gctctgcccg gggcgccagc atgggggcag catggcgtca ccgttgggaa
     3541 tccgccgcac gagacaaaac gaaacacggc gatacggcgt tgaaagcatg cgcgggttcc
     3601 gacggggcgt aatgaggacg tggcgaaacg acgagaggtc gccacgtcac ccaacccaag
     3661 gaaagatcat gaagaagacc tggctgaagc gcaccctgct gggtgtggcc gtctccaccg
     3721 ccctcctggg cagcgtcgcc gcgtactcgc aaggcagcgg ctttcaccac ggcccgccga
     3781 cgcccgagca gatcgcccag cacgagcagc acatgctcga gcacgtcggc aagaagctca
     3841 acctggacgc caaccagtcc gccaagctca aggcgctggc cgacctggcg atcgccgacc
     3901 acgcgccgcc ggccgccggc accgacccgc acgcgaagat ggccgcgctg atctccggca
     3961 gcaccttcga ccgcgccggc gcgcaggcgc tggccaacca gcacgtggcc aagatgcagg
     4021 ccgacgcccc gggcctgatc aacgcgttcg gcgacttcta cgacagcctc aacgccaccc
     4081 agcaggcgca ggtgcgcgag ctggccgccc accaccatgg cttcttcatg atgggcatgg
     4141 gcttcggcgg ccacgagggc ccgcacggtg cccacggcat gcacggcgac gcctcgggcg
     4201 cgaccggcgg ctgacgccct cccggcgagg cgtcaacgcg ccctgccgcc atccgcgcga
     4261 ggcagcggcc gcgaggtcct gcctcgccgc cgtttccgcc ctaccatcgc cgcatgcgcc
     4321 gcctcctgat gatcgacgac gacgcgctgc tcgccgcgcc gctcgccacc tatttccgcc
     4381 gcttcgacta cgacctcgac agcgccacca cgcccagcga gggcctggcc aagctgcgcg
     4441 cggcgccgta cgacgcggcc atcctcgacg tgatgctgcc cgagatggac ggcttcgagc
     4501 tgtgccgcac gatccggcgc gagtccgaca tccccatcgt gatgctgacc gcgcgcggcg
     4561 acgtgatgga ccgcatcgtc ggcctggaga tcggcgccga cgactacatc cccaagccgt
     4621 tcgagccgcg cgagctgctc acgcgcatcc agaccatcct gcgccgcgcc cgcgcgccgg
     4681 ccgccgcccc gcagggccgg ctcgtcttcg acggcctgga gatcgacctc gagcggcgcc
     4741 aggtcgtgcg ccagggcgag ccggtcgagc tcacgggcac cgaattcgag ctgctggccc
     4801 tgctcgccaa ggcgccgcag aaggtgttcc atcgcgacga gatcctgaac cggctgcggg
     4861 gccaggacgc cgacctgtac acgcgcgccg tcgacatcct cgtgagccgg ctgcgcagga
     4921 agctcgagcc gctggactgc atcaagacgc tgcgcaacgc gggctatgct tttgcgggcg
     4981 ggcatgtggc atgagcccgc acggccgccc gaagggcgaa ttgaccggag tccgcaggac
     5041 ggaggtggcc tggtgacccc gcacggc
//
LOCUS       DATCRO010000985         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_405002, whole genome shotgun sequence.
ACCESSION   DATCRO010000985 DATCRO010000000
VERSION     DATCRO010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405002"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1329)
                     /locus_tag="VIN75_26945"
     CDS             complement(109..1329)
                     /locus_tag="VIN75_26945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8977881.1"
                     /translation="MRGRVDAHVCVVGGGFAGLATAIGLIERGQRDVVVVEGETVGHG
                     ASGRNGGFVFGGFSLDNAQLLADQGRDGARAMYGLTRDAVHTIRRRIAQYGIACDAVE
                     GGVVLANWFDDDAILRKRQEFMAREYDVHWQLLSHDELGAFTTSSRYAGGLLEPDAFH
                     FHPLKYARGLAAAIEREGGRIYERSPVTRIVPPRTPGGGFEVHADGGVVHARHVVICG
                     GGYLRGVSAALERAMLPIATYVMATEPLEGALARSIPGKAAIYDTRFSFDYYRALPDT
                     RILWGGRISIIDRGAEPIARLLKRDMLKVYPELADVKVDYAWGGLMSYARHQMPQIGR
                     LPDGCWHALGFGGHGVGPTTAAGELLADAIAAGIPIPRGFADYGLQRVWGKAGMLAAQ
                     MTYNWAELKDWING"
     gene            complement(1393..1881)
                     /locus_tag="VIN75_26950"
     CDS             complement(1393..1881)
                     /locus_tag="VIN75_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY8977882.1"
                     /translation="MRIVLRLPQVLVAVGLVSLGAVGVAAIAQHVFDMQPCPWCVLQR
                     LIYIVVGVLALVAAALPGLGRRLFIALALLGTLCGLASALWQQLHAVNEASCAMTLAD
                     RIMTRLQLDALVPQLFTAYASCADAAVSVLGIPFAVWSCLLYVILGLLLVWTLRVSLK
                     SR"
     gene            complement(1878..2339)
                     /locus_tag="VIN75_26955"
     CDS             complement(1878..2339)
                     /locus_tag="VIN75_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8977883.1"
                     /translation="MTIAARLQALGIELPPLAIPAAAYLPFVQTGSLVFISGHIAKKD
                     GKPWVGQLGLTMTTEEGKAAARAIAIDLMGTLQAAAGGDLARVKRIVKVMSLVNSTPT
                     FTEQHLVTNGCSELLGEVFGDAGKHARSAFGVAQIPMGACVEIELIAELAA"
BASE COUNT          377 a          884 c          945 g          330 t
ORIGIN      
        1 gtcttccgcc agcaaggcaa catgtcggtg acgctggtgg cgctcgacca gcggcgtccg
       61 ccggtgacgg gcaacgatcg ccgctggtga agcgggcggg ccggatggtc agccgttgat
      121 ccagtccttg agttcggccc agttgtaggt catctgggcc gccagcatgc cggccttgcc
      181 ccacacgcgc tgcaggccgt aatcggcgaa gccgcgcggg atcgggatgc cggcggcgat
      241 ggcgtcggcc agcagctcgc ccgcggcggt ggtgggtccg acgccgtggc cgccgaagcc
      301 cagcgcatgc cagcagccgt cgggcaggcg gccgatctgc ggcatctggt ggcgtgcgta
      361 gctcatgagg ccgccccagg cgtagtccac cttcacgtcg gccagttcgg ggtagacctt
      421 gagcatgtcg cgcttgagca gcctcgcgat gggctctgcc ccgcggtcga tgatggagat
      481 gcggccgccc cacaggatgc gggtgtcggg cagggcgcgg tagtagtcga acgagaagcg
      541 cgtgtcgtag atcgccgcct tgccggggat cgagcgcgcg agcgcgccct cgagcggctc
      601 ggtggccatc acgtaggtgg cgatcggcag catcgcgcgc tccagcgcgg ccgacacgcc
      661 acgcaggtag ccgccgccgc agatgacgac gtggcgcgcg tgcacgacgc cgccgtccgc
      721 gtgcacctcg aagccgccgc cgggcgtgcg cggcggcacg atgcgcgtca cgggcgagcg
      781 ttcgtagatg cggccgcctt cgcgctcgat ggccgcggcc aggccgcgcg cgtacttcag
      841 cggatggaag tggaacgcgt cgggctccag caggccgccg gcgtagcgcg acgacgtcgt
      901 gaaggcgccc agctcgtcgt gcgacagcaa ctgccagtgc acgtcgtact cgcgcgccat
      961 gaactcctgg cgcttgcgca ggatcgcgtc gtcgtcgaac cagttggcca gcacgacgcc
     1021 gccctcgacg gcgtcgcagg cgatgccgta ctgcgcgatg cggcggcgga tggtgtgcac
     1081 cgcgtcgcgg gtgagaccgt acatcgcgcg ggccccgtcg cggccctggt cggccagcag
     1141 ttgcgcgttg tcgaggctga agccgccgaa cacgaagccg ccgttgcggc ccgaggcgcc
     1201 gtggccgacg gtctcgccct cgacgaccac gacgtcgcgc tgcccgcgct ctatcaggcc
     1261 gatggcggtg gccaggccgg cgaaaccgcc gccgaccacg cacacgtgcg cgtccacgcg
     1321 cccgcgcagc gcgtcacgcg cgggcgctcg gcccgcggtc gcggcgtagt acgtttccgg
     1381 gttcacgcag ggtcagcgcg acttgaggga cacgcgcagc gtccacacga gcagcagccc
     1441 gaggatcacg tacagcaggc agctccagac cgcgaacggg atgcccagca ccgacaccgc
     1501 cgcgtccgcg cacgaggcat acgcggtgaa cagctgcggc accagggcat cgagctgcag
     1561 cctggtcatg atgcgatcgg cgagcgtcat ggcgcacgac gcctcgttga ccgcatgcag
     1621 ctgctgccac agcgcggagg ccaggccgca gagcgtgccc agcaacgcca gcgcgatgaa
     1681 cagccgccgg ccgagcccgg gcagcgccgc ggccaccagc gccagcacgc ccaccacgat
     1741 gtagatcagg cgctgcagca cgcaccacgg gcagggctgc atgtcgaaca cgtgctgcgc
     1801 gatcgcggcc acgcccaccg cgccgagcga gacgaggccg acggcgacca gcacctgcgg
     1861 caggcgaagg acgatcctca tgccgcgagc tcggcgatca gctcgatctc gacgcaggcg
     1921 cccatcggga tctgcgccac gccgaaggcg ctgcgcgcgt gcttgccggc gtcgccgaag
     1981 acctcgccca gcagctcgga gcagccgttg gtgaccaggt gctgctcggt gaacgtgggc
     2041 gtgctgttga ccaggctcat caccttgacg atgcgcttga cgcgggcgag gtcgccgccg
     2101 gccgcggcct gcagcgtgcc catcaggtcg atggcgatgg cgcgcgcggc ggccttgcct
     2161 tcctcggtgg tcatcgtcag gcccagctgg ccgacccagg gcttgccgtc cttcttggcg
     2221 atgtggcccg agatgaacac gagcgagccg gtctgcacga acggcaggta ggcggcggcg
     2281 gggatggcga gcggcggaag ctcgattccg agggcttgca ggcgggcggc gatcgtcatg
     2341 aaaggtctcc gtgcgggggc gggcgccccg tgatgggcac ggatgctaca cgcctgcgca
     2401 aacacttgtc cgcgcgcggg acgcgggtgg cggcgcggag gggacgagcg gcggatggcc
     2461 gggcgcgagc ggctcctgcc gccgcttcag tcgaccgcca gcagcggcgc cagcgcctgc
     2521 cggccgggcg gcgtca
//
LOCUS       DATCRO010000986        13960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_955721, whole genome shotgun sequence.
ACCESSION   DATCRO010000986 DATCRO010000000
VERSION     DATCRO010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955721"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..495)
                     /locus_tag="VIN75_26960"
     CDS             complement(229..495)
                     /locus_tag="VIN75_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977884.1"
                     /translation="MNWYCVIIADGEPRQPRAVDFVLALRSAWRDAGRPRGAAAFINR
                     GSAARFTFLLSPEAATLAPTLLRHYDALACEQAPNLRRYAPLPL"
     gene            complement(640..834)
                     /locus_tag="VIN75_26965"
     CDS             complement(640..834)
                     /locus_tag="VIN75_26965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977885.1"
                     /translation="MTKTLTAHAAPIALAALLSTLMLFATNALAGHQYRVAVAAHETA
                     QPFASADVQRVVVVARRANT"
     gene            1086..1727
                     /locus_tag="VIN75_26970"
     CDS             1086..1727
                     /locus_tag="VIN75_26970"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8977886.1"
                     /translation="MTTATELPPGLFSHDDGVLRCQWCRASDEYRRYHDEEWGFPVTD
                     ERRLFEKICLEGFQAGLSWLTILNKRPGFRKAFANFDAEKIVRFDDADIERLVADPGI
                     VRHRGKIVSTINNARRVLELRQEFGSLAAYAWRFEADPATRPEKLLPGTALGPSAEAV
                     AMSKDLKKRGWTFVGPTTVYAFMQAMGLVNDHYEQCHAREKALAARRKLKRPE"
     gene            complement(1976..3100)
                     /locus_tag="VIN75_26975"
     CDS             complement(1976..3100)
                     /locus_tag="VIN75_26975"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY8977887.1"
                     /translation="MPVPPAATPFPPSSAIDADAPCPIPPAADARVAMAYAQTAVATA
                     QSLGVTPAQLCARLGWAEDMLDASRDSLTVAEYIALLGAGDALAGDPVFGLHTGLHAR
                     LSTYPFYGLVVCACTTLRGAFVQTRRYEGLAHDLGRSRLDERGGIATYYWDTPWLPEC
                     TPAQARHLVDSVLAGILTFVSWLAHERLPVHEIGLPYPEPDAAIRAAHAQVFGPAVVY
                     GVPAAYGRFDAALLDRPVPNADGSMLKVLQRHADELLAARLRATSEPRIVTDVRREVA
                     AQLASDRARVDDVAAALSLSTRTLQRKLADAGTNFQAVHDAARQALAEELLRDSQLNL
                     TDVAFMLGYREQSSFNRACREWFGATPARTRERLRGAAER"
     gene            3236..4849
                     /locus_tag="VIN75_26980"
     CDS             3236..4849
                     /locus_tag="VIN75_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8977888.1"
                     /translation="MTTERRWNGWGDARVQEALKPEAHAFLAQAIGRPQPVPDAALAA
                     ALAQVEAQPSRLPRHPLVDTRAETRLRASFGQSMHDWLRLRFGVLGRVTDGVAFPGSA
                     AEVRMLLTWACEQGVQVLPLGGATSVAGHLTPVGDAPVLTLGMSRMMRLLDLDPVAQL
                     ARFEAGVAGPDLEAQLRARGFTLGHFPQSFELSTLGGWVVTRSSGQQSARYGRIEQLF
                     AGGTLQTPEREWTLPTFPASAAGPDLREWVLGSEGRIGVLTEATVRVTRAPAAERFVG
                     VFFPDWASAAAAVRRVAQARLGLSMLRLANPAETHTTLTMAGHASAIAWLERYLSLRG
                     VGEGKCLLLAGFTGDSAGAVCAMQRAAASIWREHGGVSTGTVLGKKWAERRFANVYLR
                     NALWDAGYAVDTMETACDWPRVDAMVQAMENAGRAALAQHGERTHCYTHLSHVYAQGS
                     SVYSTFVYRIGADYDTALARWQSLKTAVGDAIVAHGGTITHQHGVGKDHARWLAAEKG
                     ATGMSAIAAMVREFDPHGVMARGNLLGDQ"
     gene            4846..5064
                     /locus_tag="VIN75_26985"
     CDS             4846..5064
                     /locus_tag="VIN75_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977889.1"
                     /translation="MSTGRRLAFARARWPVAVAMRAVAAALAEAGPRSSARRSVFNGS
                     RDARRVAARRDGTITRALAVPRGCEDLH"
     gene            5123..6793
                     /locus_tag="VIN75_26990"
     CDS             5123..6793
                     /locus_tag="VIN75_26990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY8977890.1"
                     /translation="MPDADILVIGGGIAGAGVALEAARRGLAVMLVEARDFAWGSSSR
                     SSKLVHGGLRYLREGAFGLTLESVRERGRLLHDAPGLVEPQRFLMGHYEGRQPTRRTL
                     GLGLAIYDAMAGARTRSFHSPAEARMLAPHLATDGLLGASGYLDAKTDDARLVLRVLQ
                     EAQRHGARVFNRAAVKSLLRAGDVDVPPSSLAAEGGGTVRPGTVVGARVEGPGFTGHV
                     RARCVINATGFWADGVRRGLGAPMLRPLRGSHLVFPLWRLPVAQSVSLLHPRDGRPVF
                     ATPWEGVALVGTTDLDHADDPDIEPRITRDETAYLLEAVRAAFPGLDLRAADAIASFA
                     GVRPVVDDRAAGGDPSKAPREHVVRDEQGLVTVTGGKLTTFRVNALDALRHAAPHLAA
                     RPQRAGFAPSLSASDLPDDDPYATPGPADFAYRPSDAILAPLADTPALAALPHGMRAR
                     LLGRHGVQAAALVREAAPADLELVPGTATPWAELHWALRHEQVRFLDDLLLRRTRLGL
                     LLPEGGAALLPRLEPVAREALRWSAGEWRDQCERYARLIDQCYTVPAE"
     gene            6790..8364
                     /locus_tag="VIN75_26995"
     CDS             6790..8364
                     /locus_tag="VIN75_26995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY8977891.1"
                     /translation="MTTDRPDDLLLSIDCGTQSTRALLFDPRGTLVAKAQVALDDYVV
                     ERPGWMSHDVEGFWLACARACRQLWAERPELAARVRGVSVTTQRGTIMPVDAQGHAVL
                     PALIWLDQRRATRPPRIGAAWRAAFRLAGVAETIRYFQREAEVNWWAENEPATWERTH
                     KVLLLSGLLNFKLTGEFVDSIGSQVGYVPFDFKAHDWAGPSDWKWQALALRREQLPEL
                     RPVGSVLGRVGAAASTATGIPAGTPVIAAAADKACEVLGSGCLEPHVGGVSYGTTATI
                     NVCTQRYVEALSFVPPYPAALPGHYDSEVQIFRGYWMVRWFKEQFGDRERNAAQERGV
                     APEALLDELVAAVPPGSLGLVLQPYWTPGIRHPGPDAKGAIIGFGDAHTRAHVYRAIL
                     EGLAYALRDGRERIERKTRVPLTELRVSGGGSQSDAALQLTADVFRLPASRPHVHETS
                     GLGAAIDCAVGLGLHGDFATAVKAMTRVERTFEPDPRNAALYDELYSQVYQKMYGRLA
                     PLYARIRAITGYPSRD"
     gene            8494..8757
                     /locus_tag="VIN75_27000"
     CDS             8494..8757
                     /locus_tag="VIN75_27000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977892.1"
                     /translation="MAFKKTMTLVALGASLVAAGAAFAGPEAGHPNIIAAREHAQMAI
                     EKMKAAQAANEYDMGGHAARAEKLLHEAEEQMRLAAEAANKHK"
     gene            complement(8821..10206)
                     /locus_tag="VIN75_27005"
     CDS             complement(8821..10206)
                     /locus_tag="VIN75_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8977893.1"
                     /translation="MNKTSVALAALTLCLTMSAHAADATPDVADKLAQQINQMQQQLD
                     AMKQELARVKAQNDALAAAQAKAAAAAPPPAPAVATAPAPAPAGGISPNLGLWGYGEI
                     YYSNPVHDHSLATADLARAVFGIGYKFDEKTSFNSEFEVEHAVASADDSGEFEVEQFY
                     VDHQLTDSLTTRMGLFLMPAGLINEHHEPTAFYGVHRNFVETLIIPSTWREGGLGLHG
                     TSDIGLTWDAGITTGLNLGGWNAMPEDPLYRTASDLGEAAPMQATHQELQNANVQHLA
                     QYAALNYTGVPNLLVGGFAFTGNMAMPSTPANLPQSRVTLWEAHARWTPGNADISAVV
                     AHGSFSNTATYNLDNAGASNPLPSSFLGYYVQGAYKAWESGSYKLAPFVRWEHYDMGA
                     SYAGIAPGFTPVPTGLASDGKPWPQPRDRVWTLGTNFYLNPNVVLKADYQRFRTNTDL
                     TRVDLGLGVSF"
     gene            complement(10212..11630)
                     /locus_tag="VIN75_27010"
     CDS             complement(10212..11630)
                     /locus_tag="VIN75_27010"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8977894.1"
                     /translation="MNVLGTRPASGARGSGLALALAALLAACGGGGSDASSPSSGGGA
                     TALSAAATLGRKIFADAALSASGQQSCASCHVASAAFAEDASASGVDHGSPVALGGPG
                     MNLTGFRNTPSLMYTSLTPAFFFDADGSPNGGFFRDGRAATLAEQATMPFTSAFEMAN
                     ADAAEVVARLKTRPYLAEFVALYGDAVLDDPQTALARMGSALAAFESEEPSFNPFSSK
                     FDAFRRGQAQLTAQELRGLGLFNNATKGNCSACHPSTSADGARPPMFTDYSYDNLGIP
                     RNPLIPGNADAAPAYTPVDSADGLHSYYDLGVCGPFRQGSENLSASCGQFKVPTLRNI
                     ALTAPYFHNGRFATLREAIGFYVRRDTNPEEWYPTAADGTVTKFDDLPAAYGGQFTVK
                     AGVVGSDAGYRGNVNTAEIPYNRSIGDTPALSPDEIDDVITFLCTLTDGFDPARPSAQ
                     VLPAQCQAAVHATAAARTSQSR"
     gene            complement(11936..12451)
                     /locus_tag="VIN75_27015"
     CDS             complement(11936..12451)
                     /locus_tag="VIN75_27015"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8977895.1"
                     /translation="MNDAPSQAPRIHLASVADVPFIVQTIVAESRAGHFSCDTDRPDV
                     VSGLWHQVQTVVTQGAMPLPGERNGVGGRAFVVQVGQINAAFAILAEHLPASWYWRLE
                     LFALSVRPDFRRHGLGRHLVTKLVHDAQSASVYARCAYGSVGMVGLLKSCGFELGAES
                     TASGMTLELRR"
     gene            complement(12575..12706)
                     /locus_tag="VIN75_27020"
     CDS             complement(12575..12706)
                     /locus_tag="VIN75_27020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977896.1"
                     /translation="MQAARDTTLRQKDLERMLHRWEVPLPREAHEVLCETGAPTAKR"
     gene            complement(12738..>13960)
                     /locus_tag="VIN75_27025"
     CDS             complement(12738..>13960)
                     /locus_tag="VIN75_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HEY8977897.1"
                     /translation="LIAPLMSRLLRFFPPVVTGTTIAIIGISLMRVGVGWAVGGPSNL
                     AQFTDVPQLVAMVEEAKVKATMDAAPGVSTEAIASGRAASVAATAAVSAAAAASAPGR
                     LAKMFAKLKLPGPVPMNDNPAYGALDNMAVSGAVLMFILLLVRFFTGFVANISVLLGI
                     VMGCVIAIAMGKMSFAKVGAAPWFDVVTPFAFGLPTFDLVSIATFTIVMVVVMIESTG
                     MFLALGDITGKKIGQADLAAGLRVDGLGTVIGGMLNTFPHTSFSQNVGLVAVTGVKSR
                     WVCVTGGFIMIVLGMLPKMAALIEAIPQFVLGGAGLVMFGMVAATGIRILATVDYKTN
                     RYNLYIVALAIGFGLVPLVAPRWMQQMPHNLHPLLESGILLTSISAVLLNLFFNAGRA
                     EAAQETTQQPIGAH"
BASE COUNT         1938 a         4920 c         5161 g         1941 t
ORIGIN      
        1 gtcgacggca tcgccacggg cacgcgcatc cgcatgggcc aggcgttgat gcgcctgccg
       61 gcgcaggcga gcgcgacgag cgcggccgaa gcgcacgagg cgctggcgcc ggcggacgcg
      121 tgacgcccgt gtgcgcgccc ctcgcgcgcg agcgcagccg cgtcgcacgc gggcgctgac
      181 gcggcgcccc ccggctcgcg tcacgcgcag gcgcgtcccg gcgcgcgctc agagcggcag
      241 cggcgcgtag cggcgcaggt tgggcgcctg ctcgcaggcc agcgcgtcgt agtggcgcag
      301 cagggtcggc gcgagcgtcg ccgcctcggg cgacagcagg aaggtgaagc gcgcggccga
      361 gccgcggttg atgaaggccg cggcgccgcg cggccgtccg gcgtcgcgcc aggccgagcg
      421 cagggccagc acgaagtcga cggcgcgcgg ctgtcgcggc tcgccgtcgg cgatgatgac
      481 gcagtaccag ttcatgggac gttcgcagga aggttcgacc tgcgatcgtg gacgcgctgc
      541 gtgacgttcc cgtgacgtgg acttcgcgtc ggccagccgg cgaacccatg aaaaaagacc
      601 ccgcagcgcg gggcccaaca ggctggtgag gccggggaat caggtgttgg cgcggcgcgc
      661 gacgaccacc acccgctgca cgtcggcgga cgcgaacggc tgcgccgtct cgtgcgccgc
      721 caccgcgacg cggtactggt gtccggccag cgcgttggtg gcgaacagca tgagcgtgct
      781 cagcagggcg gccagggcga tgggggcggc gtgggcggtg agggtcttgg tcatggtcgg
      841 ctccgtgatt tgtcgtgttg cttgatgcga tggttggact gtaggcagcg cgtctgctgc
      901 ctgccagggg cgtgcgacgg cacgcgagcc ccggcggacg aactgcaggc tggcgcgacg
      961 gaccgacgcc gcgccgcgcg ccgaacgggc gattccgccg acgaaatgcc gccgccgccc
     1021 ttctcgcccc tggttcgcgc gcttcgctgc ccggcgattc gccccggccg ctgcagaatg
     1081 gccccatgac caccgccacc gaactcccac cgggcctctt ctcccacgac gacggcgtgc
     1141 tgcgctgcca gtggtgccgt gccagcgacg agtaccgccg ctaccacgac gaggagtggg
     1201 gctttcccgt caccgacgag cgccgcctgt tcgagaagat ctgcctggag ggcttccagg
     1261 ccggcctgag ctggctgacc atcctcaaca agcggcccgg cttccgcaag gccttcgcca
     1321 acttcgacgc ggagaagatc gtgcgcttcg acgacgccga catcgagcgc ctggttgccg
     1381 accccggcat cgtgcgccac cgcggcaaga tcgtctccac catcaacaac gcccggcgcg
     1441 tgctggagct tcgtcaggag ttcggctcgc tcgccgccta cgcctggcgc ttcgaggccg
     1501 accccgcgac gcgtccggag aagctgctgc ccggcacggc actgggtccg tcggcggaag
     1561 ccgtggccat gtccaaggac ctgaagaagc gcggatggac gttcgtcggg ccgaccacgg
     1621 tttatgcgtt catgcaggcg atgggcctgg tcaatgatca ctacgagcaa tgccatgcgc
     1681 gcgagaaggc gctggcggcg cgcaggaagc tcaagcgacc ggagtgaccg gcccgcttga
     1741 agcggttgtc atttcgcgcc gagcgaagcg gagtcgcaga tccgactttc ggacgacggg
     1801 cgtcgtgatg cgtggatcct gcgactgcgc gcaggatgac ggggcttcca accggcgcgc
     1861 gggatgacgg cggtgtccga ctgcgtgcgg gatgacgacg gtgtacgacg gcgcgcagca
     1921 tgaaggcggt gtccgactgc gcgcgtgcga gccgcgcgcc tgcgtccaca tcgcgtcagc
     1981 gctcggccgc cccgcgcagg cgctcccgcg tgcgcgccgg cgtcgccccg aaccactccc
     2041 tgcatgcccg gttgaacgag ctctgctcgc gataccccag catgaacgcc acgtccgtca
     2101 ggttcagctg cgaatcgcgc agcagttcct cggcgagcgc ctgccgggcc gcgtcgtgca
     2161 cggcctggaa gttggtgccg gcgtcggcga gcttgcgttg cagcgtgcgg gtgctgagcg
     2221 acagcgcggc ggcgacgtcg tcgacgcgcg cccggtcgct ggcgagctgg gcggcgacct
     2281 cgcggcggac gtcggtgacg atgcgcggct cggacgtcgc gcgcagccgg gcagccagca
     2341 gctcgtcggc gtggcgctgc agcaccttga gcatgctgcc gtcggcgttg ggcaccgggc
     2401 ggtcgagcag cgcggcgtcg aagcggccgt aggcggccgg cacgccgtag acgaccgcgg
     2461 ggccgaacac ctgggcatgg gcggcgcgga tcgcggcgtc gggttccggg tagggcaggc
     2521 cgatctcgtg caccggcagg cgctcgtgcg ccagccagct gacgaaggta aggatgccgg
     2581 ccagcacgct gtcgacgagg tggcgcgcct gcgccggcgt gcactcgggc agccagggcg
     2641 tgtcccagta gtaggtggcg atgccgccgc gctcgtccag ccgcgagcgg cccaggtcgt
     2701 gcgccaggcc ttcgtagcgg cgcgtctgca cgaaggcgcc gcgcagcgtc gtgcaggcgc
     2761 agacgaccag gccgtagaac ggataggtcg acagccgcgc gtgcaggccc gtgtgcaggc
     2821 cgaacaccgg gtcgcccgcc agcgcgtcgc cggcgcccag cagggcgatg tactcggcga
     2881 ccgtgaggct gtcgcgcgag gcgtccagca tgtcctcggc ccacccgagc cgggcgcaca
     2941 actgcgcggg cgtgacgccc agcgactgcg cggtggcgac cgccgtctgc gcgtaggcca
     3001 tggccacgcg cgcgtcggct gccggcggga tggggcaggg cgcgtcggcg tcgatcgcgg
     3061 aggagggcgg gaacggcgtc gccgcgggcg ggactggcat gttgtggcaa ctcgcctgtc
     3121 ggcgaaagac aagccgcgac ggcgcgttcg acgcaccatg gcggcgtagc gtccggggcc
     3181 gtctgtgtcg agctccgggg gcgcctgtcg acctggaaaa acgagtatag ccacgatgac
     3241 caccgaacga cgctggaacg gctggggtga cgcccgcgtc caggaagcgc tcaagccgga
     3301 agcgcatgcc ttcctcgcac aggccatcgg ccggccccag cccgtgcccg acgcggccct
     3361 ggccgcggcg ctggcccagg tcgaggcgca gccctcgcgc ctgccgcggc atccgctggt
     3421 cgacacccgc gccgagacgc gcctgcgcgc cagcttcggc cagtcgatgc acgactggct
     3481 gcgcctgcgc ttcggcgtgc tgggccgcgt gaccgacggc gtcgccttcc cgggcagcgc
     3541 cgccgaggtg cgcatgctgc tgacctgggc ctgcgagcag ggcgtgcagg tgctgccgct
     3601 gggcggcgcg accagcgtcg cgggccacct gacgcccgtc ggcgatgcgc cggtgctcac
     3661 gctgggcatg agccgcatga tgcggctgct cgatctcgac cccgtcgcgc agctcgcgcg
     3721 attcgaggcg ggcgtcgcgg gccccgacct cgaggcgcag ctgcgcgccc gcggcttcac
     3781 gctgggccac ttcccgcaga gcttcgagct gtcgacgctg gggggctggg tggtgacacg
     3841 ctcgtcgggc cagcagtcgg cacgctacgg ccgcatcgag cagctgttcg ccggcggcac
     3901 cttgcagacg cccgagcgcg agtggacgct gcccaccttc ccggcatcgg ccgcgggccc
     3961 cgacctgcgc gagtgggtgc tgggctccga aggccgcatc ggcgtgctga ccgaggccac
     4021 cgtgcgcgtg acgcgcgcgc cggccgccga gcgcttcgtc ggcgtcttct tccccgactg
     4081 ggccagcgcc gccgcggcgg tgcggcgcgt cgcgcaggcc aggctgggcc tgtcgatgct
     4141 gcgcctggcc aatccggccg agacccacac gacgctcacg atggccggcc acgcctcggc
     4201 catcgcctgg ctggagcgct acctgtcgct gcgcggcgtg ggcgagggca agtgcctcct
     4261 gcttgccggc ttcacgggcg acagcgccgg cgccgtgtgc gcgatgcagc gcgcggccgc
     4321 atcgatctgg cgcgagcatg gcggcgtatc gaccggcacg gtgctcggca agaagtgggc
     4381 cgagcgccgc ttcgccaacg tctacctgcg caacgcgctg tgggacgccg gctatgcggt
     4441 cgacacgatg gagacggcgt gcgactggcc gcgcgtcgac gcgatggtgc aggcgatgga
     4501 gaacgccggc cgcgccgcgc tcgcgcagca tggcgagcgc acgcactgct acacgcacct
     4561 gtcgcacgtc tacgcgcagg gctcgagcgt gtactcgacc ttcgtctatc gcatcggtgc
     4621 cgactacgac acggcgctcg cgcgctggca gtcgctcaag accgcggtgg gcgacgcgat
     4681 cgtcgcgcac ggcggcacca tcacgcatca gcacggcgtc ggcaaggacc acgcgcgctg
     4741 gctggcggcc gagaagggcg cgacggggat gtcggccatc gcggcgatgg tgcgcgagtt
     4801 cgatccgcac ggggtgatgg cgcgcggcaa cctgctcggc gaccaatgag cacgggacgc
     4861 cgcctcgcct tcgcgcgcgc ccgctggccc gtggccgtcg cgatgcgcgc cgtcgcggcc
     4921 gcgctggccg aggccggccc gcgttcgagc gcgcgccgca gcgtcttcaa cggctcgcgc
     4981 gatgcgcgcc gcgtcgccgc ccggcgcgac ggcacgatca cgcgcgccct ggcggtgccg
     5041 cgcggctgcg aggacctgca ttgatcgccg cccggccgcc cgaaggcgat cgccccgcag
     5101 tccgcaggac ggaggctgcc tgatgcccga tgccgacatc ctcgtgatcg gcggcggcat
     5161 cgccggcgcg ggcgtcgcgc tggaggccgc gcggcgcggg ctggcggtga tgctggtcga
     5221 ggcgcgcgac ttcgcgtggg gcagctcgtc gcgctcgtcc aagctcgtgc acggcggcct
     5281 gcgctacctg cgcgagggcg cgttcggcct cacgctggaa tcggtgcgcg agcgcggccg
     5341 cctgctgcat gacgcgcccg gcctggtcga gccgcagcgc ttcctgatgg gccactacga
     5401 gggccgccag ccgacgcgcc gcacgctggg cctggggctg gcgatctacg acgcgatggc
     5461 cggcgcgcgc acgcgttcgt tccattcgcc cgccgaggcg cgcatgctcg cgccgcacct
     5521 ggccaccgac ggcctgctgg gcgcctcggg ctacctcgac gccaagaccg acgacgcgcg
     5581 cctggtgctg cgcgtgctgc aggaggcgca gcgccacggc gcgcgcgtgt tcaaccgcgc
     5641 ggcggtcaag tcgctgctgc gcgcgggtga tgtcgacgtg cctccgtctt cgctcgcggc
     5701 cgagggcggc ggcaccgtca ggcccggcac cgtggtgggc gcgcgcgtcg agggtccggg
     5761 gttcaccggc cacgtgcggg cgcgctgcgt catcaacgcc accggcttct gggccgacgg
     5821 cgtgcgccgc gggctcggcg cgccgatgct gcgcccgctg cgcggcagtc atctggtgtt
     5881 cccgctctgg cggctgccgg tggcccagtc ggtgagcctg ctgcacccgc gcgacggccg
     5941 gccggtgttc gccacgccgt gggaaggcgt cgcgctcgtg ggcaccaccg acctcgacca
     6001 cgccgacgat cccgacatcg aaccgcgcat cacccgcgac gagacggcct acctgctcga
     6061 ggccgtgcgc gccgcgttcc cggggctgga cctgcgtgcc gccgatgcca tcgccagctt
     6121 cgccggcgtg cgtccggtgg tcgacgaccg cgccgccggc ggcgacccgt cgaaggcccc
     6181 gcgcgagcac gtcgtgcgcg acgagcaggg cctggtcacc gtcaccggcg gcaagctgac
     6241 caccttccgc gtcaacgcgc tcgacgcgct gcgccacgcc gcaccgcacc tggcggcgcg
     6301 gccgcagcgc gccggcttcg cgccgagcct gtcggcgagc gacctgcccg acgacgaccc
     6361 gtacgcgacg ccgggccccg ccgacttcgc gtaccgcccg tccgacgcca tcctcgcgcc
     6421 gctcgccgac acccccgcgc tcgctgcact gccgcacggc atgcgcgcgc gcctgctggg
     6481 acgccatggc gtgcaggccg cggcgctggt gcgcgaggcc gcgccggccg acctcgagct
     6541 cgtgccgggc accgccacgc cgtgggccga actgcattgg gcgctgcgac acgaacaggt
     6601 gcgcttcctg gacgacctgc tgctgcgccg cacgcgcctg ggcttgctgc tgcccgaagg
     6661 cggcgcggcg ctgctgccgc ggctcgagcc cgtcgcccgc gaggccctgc gctggtccgc
     6721 cggagaatgg cgcgaccagt gcgagcgcta cgcccgcctc atcgaccagt gctacacggt
     6781 gcctgctgaa tgaccaccga ccgacccgac gacctgttgc tgtccatcga ctgcggcacc
     6841 cagagcacgc gcgcgctgct gttcgacccg cgtggcaccc tggtggccaa ggcgcaggtc
     6901 gcgctcgacg actacgtggt ggagcggccc ggctggatgt cgcacgacgt cgaaggcttc
     6961 tggctcgcct gcgcgcgcgc gtgccgccag ctgtgggccg agcgccccga gctcgcggcg
     7021 cgcgtgcgcg gcgtctcggt gacgacgcag cgcggcacga tcatgccggt ggacgcgcag
     7081 ggccacgcgg tgctgccggc gctgatctgg ctggaccagc gccgcgccac gcgcccgccg
     7141 cgcatcggcg ccgcctggcg cgcggcgttt cgcctcgccg gcgtggccga gaccatccgc
     7201 tacttccagc gcgaggccga ggtcaactgg tgggccgaga acgagcccgc cacctgggag
     7261 cgcacgcaca aggtgctgct gctgtcgggc ctgctgaact tcaagctgac gggcgagttc
     7321 gtcgactcga tcggctctca ggtgggctac gtgcccttcg acttcaaggc gcacgactgg
     7381 gccgggccgt cggactggaa gtggcaggcg ctggcgctgc gccgcgagca gctgcccgag
     7441 ctgcggccgg tgggctcggt gctgggccgg gtcggggcgg cggcgagtac cgccacgggt
     7501 atcccggcgg gcacgccggt catcgcggcc gccgccgaca aggcctgcga ggtgctgggc
     7561 tcgggctgcc tggagccgca cgtcggcggc gtgtcgtacg gcaccactgc gacgatcaac
     7621 gtctgcacgc aaaggtacgt cgaggcgctg tcgttcgtgc cgccgtaccc tgccgcgctg
     7681 ccgggccact acgacagcga ggtgcagatc ttccgcggct actggatggt gcgctggttc
     7741 aaggagcagt tcggggaccg cgagcgcaac gcggcgcagg aacgcggcgt cgcgcccgag
     7801 gcgctgctcg acgagctcgt cgcggccgtg ccgcccggct cgctgggcct ggtgctgcag
     7861 ccgtactgga cgcccggcat ccgccatccg ggacccgacg ccaagggcgc gatcatcggc
     7921 ttcggcgacg cccacacacg ggcgcacgtc tatcgcgcga tcctcgaggg cctggcctac
     7981 gcgctgcgcg acggccgcga gcgcatcgag cgcaagaccc gcgtgccgct gaccgagctg
     8041 cgcgtgtcgg gcggcggctc gcagtcggac gccgcgctgc aactgacggc cgacgtcttc
     8101 cgcctgcccg cgagccggcc gcacgtccac gagaccagcg gcctgggcgc ggcgatcgac
     8161 tgcgcggtgg gcctgggcct gcacggcgac tttgccaccg cggtcaaggc gatgacgcgc
     8221 gtcgagcgca cgttcgaacc ggacccgcgc aacgcggcgc tgtacgacga actctactcg
     8281 caggtctacc agaagatgta cggccgcctc gcgccgctgt acgcgcgcat ccgcgccatc
     8341 accggctatc cgtcgcgcga ctgacgccgg gccgcaagcc tggcctcagc ttcctgcggg
     8401 tagtccctgt gcacgggctg ctccgtggcc gcgttgtggc ggcagtccgt atccgtccac
     8461 accggcgagc ggacgccacg cacaggagac atgatggcct tcaagaagac gatgacgctg
     8521 gtcgcgctcg gcgcgtccct ggttgccgcg ggtgcggcgt tcgcgggtcc ggaagccggc
     8581 caccccaaca tcatcgccgc gcgcgagcat gcgcagatgg cgatcgagaa gatgaaggcg
     8641 gcgcaggccg ccaacgaata cgacatgggc ggccacgccg ccagggccga gaagctgctg
     8701 cacgaggccg aggagcagat gcgcctggcg gccgaggcgg ccaacaagca caagtgagcg
     8761 gcacgaccgc gccatgaaag agggatcgcc gcggcgatcc ctggtgcatg gcgcctcgcg
     8821 tcagaagctg acgcccaacc ccaggtcgac ccgcgtcagg tccgtgttcg tgcggaagcg
     8881 ctggtagtcc gccttgagaa cgacgttcgg gttcaggtag aaattcgtgc ccaacgtcca
     8941 cacgcggtcg cgcggctgcg gccagggctt gccgtccgac gccaggccgg tgggcaccgg
     9001 cgtgaagccc ggggcgatgc cggcgtagct ggcgcccatg tcgtagtgct cccaccgcac
     9061 gaacggggcc agcttgtagc tgccgctctc ccacgccttg tacgcgccct gcacgtagta
     9121 gccgaggaag gacgacggca gcgggttgga tgcgccggcg ttgtccaggt tgtacgtggc
     9181 cgtgttcgag aacgaaccgt gcgcgaccac tgccgagatg tcggcgttgc ccggcgtcca
     9241 gcgcgcatgc gcctcccaca gcgtgacgcg cgactgcggc aggttggccg gcgtcgacgg
     9301 catggccatg ttgccggtga acgcgaagcc gccgaccagc aggttgggca cgcccgtgta
     9361 gttcagggcg gcgtactgcg ccaggtgctg cacgttggcg ttctgcagtt cctggtgcgt
     9421 ggcctgcatc ggcgcggctt cgccgaggtc cgaggcggtg cggtacagcg gatcctcggg
     9481 catcgcgttc cagccgccca ggttcaggcc ggtggtgatg ccggcgtccc aggtcaggcc
     9541 gatgtcgctc gtgccgtgca ggcccaggcc gccttcacgc caggtgctgg ggatgatcag
     9601 cgtctcgacg aagttgcggt gcacgccata gaaggcggtg ggctcgtggt gctcgttgat
     9661 caggcccgcg ggcatcagga acaggcccat ccgcgtcgtg agcgagtcgg tgagctggtg
     9721 gtcgacgtag aactgctcca cctcgaactc gccggagtcg tcggccgagg ccacagcgtg
     9781 ctccacctcg aactcggagt tgaagctggt cttctcgtcg aacttgtagc cgatgccgaa
     9841 gacggcgcgc gccaggtcgg cggtggccag gctgtggtcg tgcaccgggt tcgagtagta
     9901 gatctcgccg tagccccaca gccccaggtt gggcgagatg ccgcccgcgg gtgcaggggc
     9961 gggcgccgtc gcgacggccg gtgccggcgg gggcgccgcg gcggcggcct tggcctgcgc
    10021 ggctgccagc gcatcgttct gcgccttcac gcgcgccagc tcctgcttca tcgcgtcgag
    10081 ctgctgctgc atctggttga tctgctgcgc gagcttgtcg gccacgtcgg gcgtggcgtc
    10141 ggcggcatgg gccgacatcg tcaggcaaag ggtcagggcc gcaagggcca cggaggtctt
    10201 gttcatggta ttcatcggct ctgggaagtc ctggcggcgg ccgtcgcgtg gacggcggcc
    10261 tggcattggg cgggcagcac ctgcgcggac ggacgcgccg ggtcgaaccc gtcggtcagc
    10321 gtgcacagga aggtgatgac gtcgtcgatc tcgtcgggcg acagcgccgg cgtgtcgccg
    10381 atcgagcggt tgtacggaat ctcggcggtg ttgacgttgc cccggtagcc cgcgtcgctg
    10441 ccgacgacgc cggccttcac ggtgaactgg ccgccatagg ccgcgggcag gtcgtcgaac
    10501 ttcgtgaccg tgccgtcggc ggcggtggga taccactcct cggggttggt gtcgcggcgc
    10561 acgtagaagc cgatggcctc cctgagcgtc gcgaagcgcc cgttgtggaa gtacggcgcg
    10621 gtcagggcga tgttgcgcag cgtgggcacc ttgaactggc cgcaactggc cgacaggttc
    10681 tcgctgccct ggcggaacgg gccgcagacg cccaggtcgt agtagctgtg caggccgtcg
    10741 gcgctgtcga cgggcgtgta ggccggcgcg gcatcggcgt tgcccgggat cagcggattg
    10801 cgcgggatgc ccaggttgtc gtagctgtag tcggtgaaca tcggcggcct ggcgccgtcg
    10861 gccgacgtgc tgggatggca cgcgctgcag ttgcccttgg tcgcgttgtt gaacaggccc
    10921 aggccgcgca gctcctgcgc cgtcagctgc gcctgcccgc gccggaacgc gtcgaacttg
    10981 ctcgagaacg ggttgaacga cggctcctcg gactcgaacg cggccagcgc gctgcccatg
    11041 cgcgcgagcg ccgtctgcgg gtcgtcgagc accgcgtcgc cgtagagcgc gacgaactcg
    11101 gcgaggtacg ggcgcgtctt caggcgcgcg accacttcgg cggcgtccgc gttggccatc
    11161 tcgaaggccg acgtgaacgg catcgtcgcc tgctcggcga gcgtggcggc gcggccgtcg
    11221 cgaaagaagc cgccgttggg gctgccgtcg gcgtcgaaga agaacgccgg cgtcagcgac
    11281 gtgtacatca gggacggcgt gttgcggaag cccgtcaggt tcatgcccgg gccgccgagc
    11341 gccaccggcg agccatggtc gacgccgctg gccgacgcgt cctcggcgaa tgcggcgctg
    11401 gccacgtggc agctcgcgca ggactgctgg ccggaggccg acagcgcggc gtccgcgaag
    11461 atcttccggc ccagcgtggc ggccgccgag agcgccgtgg cgccgccgcc ggatgacggg
    11521 gaggaggcgt cgctgccgcc gccgccgcag gccgcgagca gcgcggccag ggccagcgcg
    11581 agacccgatc cgcgggcgcc ggaggcgggg cgggtgccga gaacgttcat cgtcttgtca
    11641 tgccgcgcga catcctcgtc gcgggtctgg attgttgatg caagtgcgag tgattcgcaa
    11701 tcacgagaac cggactctaa cgggaacgcc tcgccgcgac aagaaacggg tgttgaaagc
    11761 ttgatcagga tccaggttgg ggcgagcctg ttgctgcgct gcaacgggcc gggcgagtca
    11821 ggttccgccg cgcgtgtcac ccgaccgcca aggactcgac agaacggtgc ctgtccccgg
    11881 accgcatcgc ccgcccggca ccgcaggcca gcacgatgcc gcgaggctcc tgcgctcagc
    11941 gccgcagctc gagcgtcatg ccgctggcgg tggattccgc gccgagttcg aacccgcacg
    12001 acttcagcaa ccccaccatg cccaccgacc cataggcgca gcgggcatag accgacgcgc
    12061 tctgcgcatc gtgcacgagc ttggtcacga gatggcgtcc gagcccgtgg cggcggaagt
    12121 ccggccgtac gctcaacgcg aacagctcca gccgccagta ccacgaggcg ggcagatgct
    12181 cggccaggat cgcgaacgcg gcgttgatct ggccgacctg cacgacgaac gcccgaccgc
    12241 ccacgccgtt gcgctccccc ggcaacggca tcgccccttg cgtgacgacg gtctgcacct
    12301 gatgccacag cccgctcacc acgtcgggcc ggtcggtgtc gcagctgaag tgccctgcgc
    12361 gcgattcggc cacgatggtc tggacgatga agggcacgtc ggcgaccgac gcgagatgga
    12421 tgcggggcgc ctgcgacggc gcgtcgttca cgggatgggc aggcgaaaga tcgagcacgg
    12481 cgacacacaa cgcgaggagg tgcgcctctt gtatagacgg ccggccggag tgcccgaacg
    12541 caagcttgcc gccgaccgtc aaaagcctgt cgcatcacct tttcgcggta ggcgcgccgg
    12601 tctcgcacag cacctcgtgc gcctcccggg gcagcggcac ttcccagcgg tgcagcatgc
    12661 gctcgagatc cttctgccgc agcgtcgtgt cgcgcgcggc ctgcatcgtc gcggcgccgc
    12721 gccgccggtc ggccgcttca gtgcgccccg atcggctgct gcgtggtctc ctgggccgct
    12781 tccgcccgcc ccgcgttgaa gaacaggttc agcagcacgg cggagatcga cgtcagcagg
    12841 atgcctgact ccagcagcgg atgcaggttg tgcggcatct gctgcatcca gcgcggggcc
    12901 accagcggga cgaggccgaa gccgatggcc agcgcgacga tgtagaggtt gtagcggttg
    12961 gtcttgtagt cgacggtcgc gaggatgcgg atgcccgtgg ccgccaccat gccgaacatc
    13021 acgaggccgg cgccgcccag gacgaactgc gggatcgcct cgatgagcgc tgccatcttg
    13081 ggcagcatgc ccagaacgat catgatgaag ccgccggtga cgcagaccca gcggctcttg
    13141 acgccggtca ccgcgacgag ccccacgttc tgagagaagc tcgtgtgcgg gaacgtgttg
    13201 agcatgccgc cgatcaccgt gcccagtccg tcgacgcgca gccccgcggc caggtcggcc
    13261 tggccgatct tcttgccggt gatgtcgccc agcgcgagga acatcccggt cgactcgatc
    13321 atcacgacga ccatcacgat ggtgaacgtg gcgatcgaga cgaggtcgaa ggtgggcagg
    13381 ccgaacgcga acggcgtgac gacgtcgaac cacggcgccg cacccacctt ggcgaagctc
    13441 atcttgccca tcgcgatcgc gatcacgcag cccatcacga tgcccagcag caccgagatg
    13501 ttggcgacga agccggtgaa aaagcgcacc agcagcagga tgaacatcag caccgcgccg
    13561 gacaccgcca tgttgtcgag cgcgccgtag gccgggttgt cgttcatggg gaccgggccg
    13621 ggcagcttca gcttggcgaa catcttcgcg aggcgtccgg gcgccgacgc cgcggcggcc
    13681 gcggacaccg ccgccgtcgc cgccaccgat gcggcgcgcc cggacgcgat cgcctcggtc
    13741 gacacgccgg gggcggcgtc catggtggcc ttgaccttcg cctcctcgac catcgcgacg
    13801 agttgcggca cgtcggtgaa ctgcgcgagg ttggacgggc cgcccacggc ccagcccacg
    13861 cccacgcgca tcaggctgat gccgatgatc gcgatggtcg tgccggtgac caccggcggg
    13921 aagaagcgca gcagccggct catcagcggc gcgatgagga
//
LOCUS       DATCRO010000987         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_944896, whole genome shotgun sequence.
ACCESSION   DATCRO010000987 DATCRO010000000
VERSION     DATCRO010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944896"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /gene="purL"
                     /locus_tag="VIN75_27030"
                     /gene_synonym="purI"
     CDS             <1..1763
                     /gene="purL"
                     /locus_tag="VIN75_27030"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY8977898.1"
                     /translation="RVKLSCNWMAACGEPGEDAALYDTVRAVGMELCPALGISVPVGK
                     DSLSMRTKWQDDGKTKQVVAPVSLIVSAFASVADVRGTRTPELAREDSALILVDLGRG
                     QNRMAGSVLAQTLQQFGDAVPDLDDPKLLVSLVDAVNALRAQGLLLAYHDRSDGGLWA
                     AACEMAFAGHSGLSLNVDMLVTEGNGIDDSRADAGDAKNWAAQVGARREELTLKALFN
                     EELGAVLQVPAAQRDAAIGVLRAHGLSAHSHVIGKPNDRGVVEVWRDAKAVFAKPLHE
                     LQQAWDDTSWRIAKLRDNPACADSEHALAGQPGDPGLHVALTFDPKENVAAPFLNLAR
                     PKVAILREQGVNSHVEMSYAMAQGGFDTYDVHMSDLHSGRVTLDQFKGFVACGGFSYG
                     DTLGAGEGWARSVMFNPVLAEQFEAFFGKTDTFALGVCNGCQMLAALSPIIPGAQDWP
                     KFTRNRSEQFEARLSLVEVLESPSIFFAGMAGSRMPIAVAHGEGFADFSQRGDASRVH
                     AAMRYVDHHGKPTETYPLNPNGSPGGLTAVTTADGRFTALMPHAERVFRNVQMSWSGG
                     DASAPSPWGRMFGNARRWIG"
     gene            1967..>2670
                     /locus_tag="VIN75_27035"
     CDS             1967..>2670
                     /locus_tag="VIN75_27035"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HEY8977899.1"
                     /translation="MNKTIVSVAAALAAAHAGAAVTTIADSALTASNHLYTSDYGQVV
                     VTTGGENGANIGDATGRNDDGFSGPVSLGYTLQNFFGADYSSIFINNNGNITFNSGVA
                     AYTPAGPQGATQPVIAPFFADVDTRGASSGVVYVNQSIANETIITWDHVSYYGSTDGK
                     LDTFQLVIRGAGYATPAGEGQIGFFYGGMNWETGDASGGSGGFGGHAAAVGFGDGQQN
                     GFVLAGSNEAGIAGIV"
BASE COUNT          424 a          954 c          922 g          370 t
ORIGIN      
        1 cgcgcgtcaa gctgtcgtgc aactggatgg ccgcgtgcgg cgagccgggc gaggacgcgg
       61 cgctgtacga cacggtgcgc gcggtgggca tggagctgtg cccggcgctg ggcatcagcg
      121 tgccggtggg caaggacagc ctgtcgatgc gcacgaagtg gcaggacgac gggaagacga
      181 agcaggtggt cgcgccggtg tcgctgatcg tgtcggcgtt cgcgagcgtc gcggacgtgc
      241 gcggcacgcg cacgcccgag ctcgcgcgcg aggactcggc gctgatcctg gtcgacctgg
      301 gccgcggcca gaaccgcatg gccggctcgg tcctggcgca gacgctgcag cagttcggcg
      361 acgcggtccc cgacctggac gacccgaagc tgctggtgtc gctggtcgac gccgtcaacg
      421 cgctgcgcgc gcaaggcctg ctgctggcgt accacgaccg cagcgacggc ggcctgtggg
      481 ccgcggcgtg cgagatggcc ttcgcgggcc actcgggcct gagcctgaac gtcgacatgc
      541 tggtcaccga aggcaacggc atcgacgaca gccgcgccga cgccggcgac gcgaagaact
      601 gggccgcgca ggtaggtgcg cgccgcgagg aactcacgct caaggcgctg ttcaacgagg
      661 agctcggcgc cgtgctgcag gtgcccgcgg cgcagcgcga cgcggccatc ggcgtgctgc
      721 gcgcgcacgg gctgtccgcg cacagccacg tgatcggcaa gcccaacgac cgcggcgtcg
      781 tcgaggtctg gcgcgacgcg aaggccgtgt tcgccaagcc gctgcacgag ctgcagcagg
      841 cctgggacga tacgtcgtgg cgtatcgcga agctgcgcga caacccggcc tgcgccgaca
      901 gtgaacacgc cctcgcgggc cagcccggcg accccggcct gcacgtcgcg ctcacgttcg
      961 atccgaagga gaacgtggcc gcgccgttcc tgaacctggc gcggcccaag gtggccatcc
     1021 tgcgcgagca gggcgtcaac agccacgtcg agatgagcta cgcgatggcc cagggcggct
     1081 tcgacaccta cgacgtccac atgagcgacc tgcactcggg ccgcgtcacg ctggaccagt
     1141 tcaagggctt cgtggcctgc ggcggcttca gctacggcga cacgctgggc gccggcgaag
     1201 gctgggcccg ctcggtgatg ttcaacccgg tgctggccga gcagttcgag gccttcttcg
     1261 gcaagaccga caccttcgcg ctgggcgtgt gcaacggctg ccagatgctg gccgcgttgt
     1321 cgcccatcat cccgggcgcg caggactggc ccaagttcac gcgcaaccgc tccgagcagt
     1381 tcgaggcccg gctgtcgctg gtggaagtgc tggagtcgcc gtcgatcttc tttgcgggca
     1441 tggccggcag ccgcatgccc atcgcggtcg cgcacggcga gggctttgcc gacttctcgc
     1501 agcgcggcga cgcttccagg gtccatgccg cgatgcgcta cgtcgaccac catggcaagc
     1561 cgaccgagac ctacccgttg aacccgaacg gcagccccgg cggcctgacg gcggtcacga
     1621 cggccgacgg ccgcttcacg gcgctgatgc cgcacgccga gcgcgtgttc cgcaacgtcc
     1681 agatgagctg gagcggcggc gacgcttctg cgcccagccc gtggggccgc atgttcggca
     1741 acgcgcggcg ctggatcggc tgacgcgcgc ggtcgccaac acgcaagggg catccggccg
     1801 ggaggcggga tgccccttgc cgtatcggca cgactcgttt cattcgtcgt cccccactat
     1861 gaggggtgtt cgagcgatcg ggtttcacca agaatcgcgc ctgctccaac aacagcagac
     1921 gaacgacacg ccgtggcgtt cgcgtgaagg gtaccctttg agcctcttga acaaaaccat
     1981 cgtctccgtc gccgcggcgc tggccgccgc ccacgccggc gccgccgtca ccaccatcgc
     2041 cgactcggcg ctgaccgcgt ccaaccacct ctacaccagc gactacggcc aggtcgtcgt
     2101 caccaccggc ggcgagaacg gcgccaacat cggcgacgcc accggtcgca acgacgacgg
     2161 cttctccggc ccggtcagcc tcggctacac gctgcagaac ttcttcggcg ccgactactc
     2221 gtcgatcttc atcaacaaca acggcaacat cacgttcaac agcggcgtcg ccgcctacac
     2281 gccggccggc ccgcaaggcg ccacccagcc cgtgatcgcc ccgttcttcg ccgacgtcga
     2341 cacgcgcggc gccagcagcg gcgtcgtcta cgtcaaccag tcgatcgcca acgagacgat
     2401 catcacctgg gaccacgtga gctactacgg ctccaccgac ggcaagctcg acaccttcca
     2461 gctggtgatc cgcggcgcgg gctacgccac gccggcgggc gagggccaga tcggcttctt
     2521 ctacggcggc atgaactggg agaccggcga cgccagcggc ggctcgggcg gcttcggcgg
     2581 ccacgccgcg gcggtcggct tcggcgacgg ccagcagaac ggcttcgtgc tggccggctc
     2641 gaacgaggcc ggcatcgccg gcatcgtcaa
//
LOCUS       DATCRO010000988         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_764821, whole genome shotgun sequence.
ACCESSION   DATCRO010000988 DATCRO010000000
VERSION     DATCRO010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764821"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VIN75_27040"
     CDS             complement(<1..2078)
                     /locus_tag="VIN75_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEY8977900.1"
                     /translation="MFDKILIANRGEIACRVAATARRLGVKTVAVYSDADASARHVAA
                     CDEAVRIGEAAPRDSYLRWERIIEAAKATGAQAVHPGYGFLSENEDFAQACADAGIAF
                     IGPPPSAIRAMGLKAESKQLMEKAGVRLVPGYHGGDQDPALLRREADRIGYPVLIKAS
                     AGGGGKGMRIVSSGDEFDAALASCKREAINSFGDDAVLVERYVTRPRHIEIQVFFDTH
                     GNGVYLFERDCSVQRRHQKVLEEAPAPGMTPERRREMGEAAVAAARAVNYVGAGTVEF
                     IVEQDGPSATQGRPKQASAPSGGSEAQPSVGVGNFYFMEMNTRLQVEHPVTEAITGHD
                     LVEWQLRVAAGEPLPATQADLQIRGHAIEARICAENPDANFLPATGTLSVLKWPQHVN
                     FRRTEAGASESAAVRVDTGVNEGDAITPYYDSMIAKLIVWGQDRAQALARLDAALAGT
                     HIAGVVNNVAFLRRTARTESFSQANLDTALIERERPALFGHAGLPLDVVAAGVAARAV
                     ALAQATVGADPWSKRDGWRMYGASVRRFDLEWQGQSLEALLESRHDGGALLKVGDHAV
                     AFASRALGDDRYDVTLGTGADTRRVALSVYQLGEQVSVFAAAGSAQVTVVDTLSHAGE
                     GHAEAGRLTAPMPGKVIAYLVRKGDAIKTGQPLAVLEAMKMEHTIAAPRDGTVAELLF
                     APGDQVSEGG"
     gene            complement(2109..>2870)
                     /locus_tag="VIN75_27045"
     CDS             complement(2109..>2870)
                     /locus_tag="VIN75_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8977901.1"
                     /translation="AALARRPDIDASRTIVMGQSHGGLATMALESFNPKGVVGLVNFA
                     GGLRLKNCDRWRTELTSAMTDFGHHAPLPSLWFYGDNDQTFDSDLWQGMFKGYTDAGG
                     QARLVAFGTFRDDAHKMFASGPGLKIWVPAVGDFFRGLGLPFDVKYRVVVADHETPPP
                     PSSGFAPIGDAARIPFLDDRGRAAWTGYLNAAPPKAFAISPKGNWYYRYGDPAAMRAA
                     VEQCGEREPRGPCQLYAVDDSVVWHAAASSTQAKN"
BASE COUNT          367 a         1033 c         1025 g          445 t
ORIGIN      
        1 tcgccgcctt cgctgacctg gtcgccgggc gcgaacagca gctcggccac ggtgccgtcg
       61 cgcggcgcgg cgatggtgtg ctccattttc atcgcttcga gaaccgccag cggctgtccg
      121 gtcttgatgg cgtcgccctt cctgacgagg tacgcgatga ccttgccggg catcggcgcg
      181 gtgaggcgcc cggcctcggc gtgcccctcg ccggcgtgcg acagcgtgtc gacgacggtg
      241 acctgcgcgc tgcccgcggc cgcgaacacg ctcacctgct cgcccagctg gtagacgctc
      301 agcgccacgc ggcgggtgtc ggcgccggtg cccagcgtga cgtcgtagcg gtcgtcgccg
      361 agcgcgcgcg acgcgaaggc gacggcatga tcgccgactt tcagcaacgc gccgccgtcg
      421 tggcgcgatt ccagcagcgc ttccagcgac tggccttgcc actccaggtc gaagcggcgc
      481 accgacgcgc cgtacatgcg ccagccgtcg cgcttcgacc atggatccgc accgaccgtc
      541 gcctgcgcca gtgcgaccgc gcgcgcagcc acgccggccg ccaccacgtc cagcggcagt
      601 ccggcgtggc cgaacagcgc gggccgctcg cgttcgatca gcgcggtgtc gaggttcgcc
      661 tgcgagaatg actcggtcct ggcggtgcgg cgcaggaagg ccacgttatt gacgacgccc
      721 gcgatgtgcg tgcccgcgag tgccgcgtcg agccgtgcca gcgcctgcgc gcggtcctgg
      781 ccccagacga tcagcttggc gatcatcgag tcgtagtacg gcgtgatcgc gtcgccctcg
      841 ttgacgccgg tgtccacgcg caccgccgcc gactcgctcg cgccggcctc ggtgcggcgg
      901 aagttcacgt gctgcggcca cttgagcacc gacagcgtgc cggtggccgg caggaagttg
      961 gcgtccgggt tctcggcgca gatgcgcgcc tcgatcgcgt ggccgcggat ctgcaggtcg
     1021 gcctgcgtcg ccggcagcgg ctcgcccgcc gcgacgcgca gctgccactc gacgagatcg
     1081 tgcccggtga tcgcctcggt caccggatgc tccacctgca gccgcgtgtt catctccatg
     1141 aaatagaaat tgccgacccc cacgctcggc tgcgcctcgc tgccccccga gggggcgctc
     1201 gcttgcttgg ggcggccctg cgtcgcgctc gggccgtcct gctccacgat gaactccacg
     1261 gtgcccgcgc cgacgtagtt gaccgcgcgc gccgccgcca cggcggcctc gcccatctcg
     1321 cggcggcgct cgggcgtcat gccgggcgcc ggcgcctcct cgagcacctt ctggtggcgg
     1381 cgctgcaccg agcagtcgcg ctcgaacagg tagacgccgt tgccgtgcgt gtcgaagaag
     1441 acctggatct cgatgtggcg cgggcgcgtg acatagcgct cgacgagcac cgcgtcgtcg
     1501 ccgaagctgt tgatcgcctc gcgcttgcac gacgcgagcg cggcgtcgaa ctcgtcgccc
     1561 gaggacacga tgcgcatgcc cttgccgccg ccgcccgcgc tcgccttgat cagcaccgga
     1621 tagccgatgc ggtcggcctc acgcctgagc agcgccggat cctggtcgcc gccgtggtag
     1681 ccgggcacca gccgcacgcc ggccttttcc atcaactgct tggattcggc cttcaggccc
     1741 atcgcgcgga tcgccgaggg cggcgggccg atgaaggcga tgccggcgtc ggcgcaggcc
     1801 tgcgcgaagt cctcgttctc ggacaggaac ccgtagcccg ggtgcacggc ctgcgcgccc
     1861 gtggccttgg cggcctcgat gatgcgctcc cagcgcaggt agctgtcgcg cggggcggcc
     1921 tcgccgatgc gcacggcctc gtcgcaggcc gcgacgtggc gcgcgctggc gtcggcatcc
     1981 gagtagacgg cgacggtctt gacgccgagg cggcgggcgg tggcggccac gcggcaggcg
     2041 atctcgccgc ggttggcaat caggatcttg tcgaacatgg tggtctttcg tgagctcggt
     2101 agtcgtcgtc agttcttggc ctgggtcgag gacgccgcgg cgtgccagac cacggaatcg
     2161 tccacggcgt agagctggca gggtccgcgc ggctcgcgct cgccgcattg ctcgaccgcc
     2221 gcgcgcatcg cggccgggtc gccgtaccgg tagtaccagt tgcccttcgg cgagatcgcg
     2281 aaggccttcg gcggcgccgc gttcaggtag ccggtccagg cggcgcgtcc gcggtcgtcg
     2341 aggaagggga tgcgagccgc gtcgccgatg ggcgcgaagc cgctcgacgg cggcggcggg
     2401 gtctcgtggt cggcgacgac gacgcggtat ttcacgtcga agggcaatcc gagtccgcgg
     2461 aagaagtcgc cgaccgccgg cacccagatc ttgaggcccg gccccgaggc gaacatcttg
     2521 tgcgcgtcgt cgcggaacgt cccgaacgcg accagccgcg cctggccgcc cgcatccgtg
     2581 tagcccttga acatgccttg ccacaggtcg ctgtcgaagg tctggtcgtt gtccccgtag
     2641 aaccacaggc tgggcagcgg cgcgtggtgc ccgaagtcgg tcatggcgct ggtcagctcg
     2701 gtgcgccagc ggtcgcagtt cttcaggcgc aggccgccgg cgaagttgac caggcccacg
     2761 acccccttcg ggttgaacga ttcgagtgcc atcgtcgcca ggccgccatg cgactgcccc
     2821 atcacgatgg tgcggctggc gtcgatgtcg ggccgccggg ccagcgcggc
//
LOCUS       DATCRO010000989         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_112375, whole genome shotgun sequence.
ACCESSION   DATCRO010000989 DATCRO010000000
VERSION     DATCRO010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112375"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2560)
                     /locus_tag="VIN75_27050"
     CDS             complement(<1..2560)
                     /locus_tag="VIN75_27050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8977902.1"
                     /translation="MSKQDGRAAEEGAFRRVIENVAAGIVVLQDERLAFVNARAARLL
                     GREAAALVGIDPATLLSGDERGRLAESYRALVDSQADEDYGEYRITLPDGRVRHVAMS
                     ATRSDWSGRPAMLGVLHDISRQRAAMAALRSSEERYRTMIESLNEGVMMFDGQARLEG
                     VNAAAERLLGVSYERMRNNRVADWQPVGLDGEVMAPDDIPIARVLATGKPVRNALLGM
                     RIPDDGVRWLEVNCEPLHDGGGAVTGGLLSFTDVTEARRVEEALRVSESTNRTLMDAL
                     ADGVFVAQDFHFVYANRLLLERLGYEREEFVGLPFVRVVAPDLLETWNERFRGRVGTG
                     PEPVRTYEVRFLRKDGSLAEFELVANRTTFQNRPAVLGVLRDIAERKRAAAELERHRD
                     RLEELVHERTRALEAAVAARIQSENFAQTMTDHQPTLLAYVDRERKVQFANRAWLNWF
                     GKTRDEVIGRDARDSVGAMMLQPDEGVVAKVMRGEAQGMAAEMRGADGRVGNFWIYRL
                     PDVVDGDIRGYFFIATDVTEMRRSERRLQELNAKLIQAESFTRGIADNLPVRVAYWDA
                     DERCRFANKVYCDWIGRSLDQVLGKTKAEIFEQEQVPERLAHARRALDGATQKFESEE
                     VSADGRYGIWEVQYIPDLQRGEVRGFFMLATDITASKRAQADLETLNLELVAARDAAE
                     EAAQAKSAFLANMSHEIRTPMNVIIGLTHLMQRDAHDAATSDRLGKVGDAAMHLLDII
                     NDILDLSKIDAGKLVLAPADFSVDALLARATALIADGARRKGLALTVDRGNLPQALRG
                     DALRLSQVLVNLLSNAVKFTSRGSVVLRCDVAGQAGSEWTIRFSVRDTGVGITRE"
     gene            complement(2768..3793)
                     /gene="egtD"
                     /locus_tag="VIN75_27055"
     CDS             complement(2768..3793)
                     /gene="egtD"
                     /locus_tag="VIN75_27055"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY8977903.1"
                     /translation="MKTDIQRAGAEHAAEPSLRYNVTPLTPAFRVDPQFEADVLEGLD
                     DDQKHIPSIWLYDRLGSEWFEEITEVAEYYPTRTETRLLAGLAGELEGESGAVRSLVE
                     IGSGSSRKTRLLLAAMPGLARYVPVDISADFLHDAVAGLARDFPHVHMQPVVADFTEA
                     FALPPEAAADPAGNLGFFPGSTIGNFTPDAAIALMTQLGRTLGPRSRLLIGVDTTQDP
                     ALLIPAYDDAAGVTAEFNLNLLDRINRELGGDFDRRDFRHLARHDPQEGRIEMHLVSQ
                     RAHEVHVHGRTFAFAEGETIHTENCYKYTPSKFLRMAHAARWRCERSWQDGGRTGFTV
                     YLLRREA"
     gene            complement(3790..5037)
                     /gene="egtB"
                     /locus_tag="VIN75_27060"
     CDS             complement(3790..5037)
                     /gene="egtB"
                     /locus_tag="VIN75_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY8977904.1"
                     /translation="MTPSIDAYRQVRAATLRLVDGLDDADLTPQSAEFASPGKWHLAH
                     TTWFFEQFVLGTYGTGYRRFDERFAFLFNSYYNAVGARHDRPRRGLLTRPSLAQVRAY
                     REHVDQAMADLLGSRLPAAAGDLVELGLHHEQQHQELILTDLLHLFAQNPLRPAVRPS
                     PEAAAPGAEAPLGWVAFDGGVVETGHAGAAFAFDNESPRHEVVLRPFRLATRPVTNRE
                     WLAFVEAGGYADPQLWLSDGWDAVQAQRWQAPLHWELAEDGRTRLQMTLAGLRPLALD
                     APVSHVSFFEADAYARWAGRRLPTEFEWERAARDVAIEGNFVETGAWAPRADAQPAAA
                     GLRQMFGDVWEWTASPYVAYPGFRPAPGAVGEYNGKFMNGQYVLRGGSCASPRSHLRA
                     SYRNFFQPGQRWQFSGLRLAEDA"
     gene            5371..5784
                     /locus_tag="VIN75_27065"
     CDS             5371..5784
                     /locus_tag="VIN75_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8977905.1"
                     /translation="MPAATTPLLSGGNPQIAKGDGDAPVQAYIAAMPGWKRGIGAQVD
                     ALVTRTVPGARKAVKWNSPFYGSADQGWFLSLHCYAKFVRLAFFQGAALRPPPPGPSK
                     QPGVRYLDLREDAAIDEAQLAEWIRQASALPGWTP"
     gene            complement(5792..6475)
                     /gene="rpe"
                     /locus_tag="VIN75_27070"
     CDS             complement(5792..6475)
                     /gene="rpe"
                     /locus_tag="VIN75_27070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8977906.1"
                     /translation="MKPTYRIAPSILSADFARLGQEFTDVIAAGADWIHFDVMDNHYV
                     PNLSFGPMICEALRKHAVKADGARVPIDVHLMVQPVDALAQAFAKAGADYISFHPDAS
                     THVDRTLQLIKGAGCKAGLVFNPASPIDVLEWVIDKVDLVLVMSVNPGFGGQSFIDSA
                     LRKIERLRKVIDASGRDIRLEVDGGIKADNIRAAADAGADTFVAGSAIFNQKDYAKAI
                     AEMRAALAA"
     gene            6583..6957
                     /gene="apaG"
                     /locus_tag="VIN75_27075"
     CDS             6583..6957
                     /gene="apaG"
                     /locus_tag="VIN75_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY8977907.1"
                     /translation="MPRPELSVSVATRFLHEQSSAADDVYAFAYTITIRNTGEVTAQV
                     IGRHWIITHGSGQVEEVRGLGVVGQQPLLKPGEEYEYTSWTRITTPRGSMRGEFLCMT
                     DAAEPFEAIVEPFPLAIAQALH"
     gene            complement(6951..>7208)
                     /locus_tag="VIN75_27080"
     CDS             complement(6951..>7208)
                     /locus_tag="VIN75_27080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977908.1"
                     /translation="GGVVVPGAAGGVRVSPGGPPYGACTAHPARVPRIAPRATAAKRD
                     LMGWMLFEAMLALAALVAAVWWTFRGRVEDDDDPESADRPQ"
BASE COUNT         1012 a         2554 c         2573 g         1069 t
ORIGIN      
        1 cctcgcgggt gatgcccacg ccggtgtcgc gcaccgagaa gcggatcgtc cattcgctgc
       61 cggcctggcc cgccacgtcg cagcgcagga cgacgctgcc gcgtgacgtg aacttcaccg
      121 cgttgctcag gaggttgacc agcacctgcg acagccgcag cgcgtcgccg cgcagcgcct
      181 gcggcaggtt gccgcgatcc accgtcaatg ccaggccctt gcggcgcgcg ccgtcggcga
      241 tcagcgccgt ggcgcgcgcg agcagcgcgt cgacggagaa gtccgccggc gccagcacca
      301 gcttgccggc gtcgatcttg gacaggtcca ggatgtcgtt gatgatgtcg agcagatgca
      361 tcgccgcgtc gcccaccttg cccagccggt ccgacgtggc cgcgtcgtgc gcgtcgcgtt
      421 gcatcaggtg cgtgaggccg atgatgacgt tcatcggggt gcggatctcg tggctcatgt
      481 tggccaggaa cgccgacttg gcctgcgccg cctcctccgc cgcatcgcgc gccgcgacga
      541 gttcgaggtt gagcgtctcg aggtcggcct gcgcgcgctt ggaggccgtg atgtcggtgg
      601 ccagcatgaa gaagccgcgc acctcgccgc gctgcaggtc ggggatgtac tgcacctccc
      661 agatgccgta gcggccgtcg gcgctgactt cctcggactc gaacttttgc gtcgcgccat
      721 cgagcgcgcg cctggcgtgg gcgagccgct cgggcacctg ctcctgctcg aagatctccg
      781 ccttggtctt gcccagcacc tggtccagcg accggccgat ccagtcgcag tagaccttgt
      841 tggcgaagcg gcagcgctcg tcggcatccc agtaggccac gcgcaccggc aggttgtcgg
      901 cgatgccgcg cgtgaacgac tcggcctgga tcagcttggc gttgagctcc tgcagccggc
      961 gctcgctgcg gcgcatctcg gtgacgtcgg tggcgatgaa gaagtagccg cggatgtcgc
     1021 cgtcgacgac gtcgggcagc cggtagatcc agaagttgcc gacgcggccg tccgcgccgc
     1081 gcatctccgc cgccatgccc tgcgcctcgc cgcgcatcac cttggcgacg acgccctcgt
     1141 cgggctgcag catcatggcg ccgaccgaat cgcgcgcgtc gcggccgatg acctcgtcac
     1201 gcgtcttgcc gaaccagttg agccaggcgc ggttggcgaa ctgcaccttg cgctcgcggt
     1261 cgacgtaggc cagcagcgtg ggctggtggt cggtcatcgt ctgcgcgaag ttctcgctct
     1321 ggatgcgcgc ggccaccgcg gcctcgagcg cccgggtccg ctcgtgcacg agctcctcga
     1381 gccggtcgcg atggcgctcg agctccgccg ccgcgcgctt gcgctcggcg atgtcgcgca
     1441 gcacgcccag caccgcgggc cggttctgga acgtggtgcg gttggccacc agttcgaact
     1501 ccgcgaggct gccgtccttg cgcaggaagc gcacctcgta cgtgcgcacc ggctcgggcc
     1561 ccgtgcccac gcggccgcgg aagcgctcgt tccaggtctc caggaggtcg ggcgcgacga
     1621 cgcggacgaa cggcagaccg acgaactcct cgcgctcgta gcccaggcgc tcgagcagca
     1681 ggcggttggc gtagacgaag tggaagtcct gcgccacgaa cacgccgtcc gccagcgcgt
     1741 ccatcagcgt gcggttggtg ctctcgctga cgcgcagcgc ctcctcgacg cggcgcgcct
     1801 cggtgacgtc ggtgaacgac agcaggccgc ccgtgacggc gccgccgccg tcgtgcagcg
     1861 gctcgcagtt gacctccagc cagcgcacgc cgtcatcggg aatgcgcatg cccagcagcg
     1921 cgttgcgcac cggcttgccg gtggccagca cgcgcgcgat cgggatgtcg tcgggcgcca
     1981 tgacctcgcc gtccaggccg acgggctgcc agtcggcgac gcggttgttg cgcatgcgtt
     2041 cgtagctcac gcccagcagg cgctcggcgg cagcgttcac gccctcgagc cgcgcctggc
     2101 cgtcgaacat catgacgccc tcgttgagcg actcgatcat cgtgcgatag cgctcctcgc
     2161 tgctgcgcaa cgcggccatc gccgcgcgtt ggcgcgagat gtcgtgcagc acgcccagca
     2221 tcgcgggccg gccgctccag tcggagcgcg tggccgacat cgcgacgtgg cgcacgcggc
     2281 cgtcgggcag cgtgatgcgg tactcgccgt agtcctcgtc ggcctggctg tcgacgagcg
     2341 cccggtacga ctcggccagc cgcccgcgct cgtcaccgga cagcagcgtg gccggatcga
     2401 tgcccaccaa cgccgcggcc tcgcggccga gcagccgcgc cgcgcgcgcg ttgacgaagg
     2461 cgagacgctc gtcctgcagg accacgattc ccgcggcgac gttctcgatc acccggcgga
     2521 acgcgccttc ctccgccgca cggccatcct gcttgctcat ctgcttgggt tctcccgcac
     2581 tgcccggtcc aggcatcggc tcacgggccg ggccttgtcc gggacagctc gaggcagttc
     2641 actccgatgc ccgcgccgtg tcaagccgta acgaccacat ccatccgtgc gaccggtcgt
     2701 cgtgcagcca tcgcaccgga cggtcggtag tatcgggcta atacggttga taccctgtac
     2761 cgtatcttca agcctcgcgc cgcagcaggt agacggtgaa gccggtgcgg ccgccgtcct
     2821 gccacgagcg ctcgcagcgc cagcgcgcgg cgtgggccat gcgcaggaac ttcgacggcg
     2881 tgtacttgta gcagttctcg gtgtggatgg tctcgccctc ggcgaacgcg aaggtgcgcc
     2941 catgcacgtg cacctcgtgc gcgcgctggc tcaccaggtg catctcgatg cggccttcct
     3001 gcgggtcgtg gcgcgccagg tggcggaagt cgcgccggtc gaagtcgccg ccgagttcgc
     3061 ggttgatgcg atcgagcagg ttgaggttga actccgcggt cacgccggcg gcgtcgtcgt
     3121 aggccgggat cagcagcgcc ggatcctgcg tcgtgtcgac gccgatcagc agccgcgagc
     3181 gcggccccag cgtgcggccc agctgcgtca tcagcgcgat ggcggcgtcg ggcgtgaaat
     3241 tgccgatggt cgagccgggg aagaagccga ggttgcccgc cgggtcggcc gcggcctcgg
     3301 gcggcagcgc gaacgcctcg gtgaagtcgg ccacgaccgg ctgcatgtgc acgtgcggga
     3361 agtcgcgcgc caggccggcc acggcgtcgt gcaggaagtc ggccgagatg tcgacgggca
     3421 cgtagcgcgc gagcccgggc atggccgcca gcagcaggcg cgtcttgcgg ctggagccgc
     3481 tgccgatctc gaccaggctg cgcacggcgc cgctctcgcc ctcgagctcg cccgccaggc
     3541 ccgcgagcag gcgcgtctcg gtgcgtgtcg ggtagtactc ggcgacctcg gtgatctcct
     3601 cgaaccactc cgagccgagg cggtcgtaca gccagatgct ggggatgtgc ttctggtcgt
     3661 cgtccaggcc ctcgagcacg tcggcctcga actgcggatc gacgcggaac gccggcgtca
     3721 gcggcgtgac gttgtagcgc agtgacggct cggccgcatg ctcggcgcct gcccgctgga
     3781 tgtctgtctt catgcgtcct ccgcgaggcg caggccgctg aactgccagc gttgcccggg
     3841 ttgaaagaag ttgcgatagc tcgcgcgcag gtggctgcgc ggcgaggcgc acgagccgcc
     3901 gcgcagcacg tactggccgt tcatgaactt gccgttgtat tcgccgaccg cgcccggcgc
     3961 ggggcggaag ccggggtagg cgacgtaggg gctggccgtc cattcccaca cgtcgccgaa
     4021 catctggcgc aggcccgcgg ccgccggctg cgcatcggcg cgcggggccc aggcgcccgt
     4081 ctcgacgaag ttgccctcga tggcgacgtc gcgcgccgcg cgctcccatt cgaactcggt
     4141 gggcaggcgc ctgccggccc agcgcgcgta cgcgtcggcc tcgaagaagc tcacgtgcga
     4201 cacaggggcg tccagcgcga gcggccgcag gccggccagc gtcatctgca gccgggtgcg
     4261 gccgtcctcg gcgagctccc aatgcagcgg cgcctgccag cgctgcgcct gcacggcgtc
     4321 ccagccgtcg gacagccaca gctgcggatc cgcatagccg cctgcctcga cgaacgccag
     4381 ccactcgcgg ttggtgaccg gccgcgtggc caggcggaac ggccgcagca cgacctcatg
     4441 gcggggcgac tcgttgtcga acgcgaacgc cgcgccggcg tggccggtct cgacgacgcc
     4501 gccgtcgaac gcgacccagc ctagcggcgc ctcggcgcca ggcgcagcag cctcgggcga
     4561 cggccgcacc gccggccgca gcgggttctg cgcgaacagg tgcagcaggt cggtgaggat
     4621 cagctcctgg tgctgctgct cgtggtgcag gcccagctcg accaagtcgc ctgccgcggc
     4681 gggcagccgg ctgcccagca ggtcggccat cgcctggtcg acgtgctcgc ggtaggcgcg
     4741 cacctgggcc agcgacgggc gggtcagcag gccgcggcgc ggacggtcgt ggcgcgcgcc
     4801 gaccgcgttg tagtacgagt tgaacaggaa cgcgaagcgc tcgtcgaagc gacggtagcc
     4861 cgtgccgtac gtgcccagca cgaactgctc gaagaaccag gtggtgtgcg cgaggtgcca
     4921 ctttccgggg ctcgcgaact cggccgattg tggcgtgagg tccgcatcgt ccaggccgtc
     4981 gaccaggcgc agcgtggcgg cacggacctg gcgataggca tcgatcgaag gcgtcatggc
     5041 aaggcgcgac gggagccgcg ggttggcgtg gattcccgac gttgacggac gtcggctcgg
     5101 ccgcatgacc gaggggccat gctaggggca aacgccgcgc cgtgctgacg ggggtcgccg
     5161 cgccaccatg gtgtcagcag ggggctttgc ggcggcggtg tcagcaggcg cgccggcatc
     5221 atcgggcttg gtcgggccaa ggtcggcaag ccttacacgc gcgcggacgc gggcgccccc
     5281 aggcgtcacg cgagcgggcg cccactagcg tgcgaccgca cgcgtgccgt cccactgaca
     5341 agcgcgccca gcccgcccac aatggtgccc atgcctgctg ccaccacccc gctgctgtcc
     5401 ggcggcaacc cgcagatcgc caagggcgac ggcgacgcgc ccgtccaggc gtacatcgcc
     5461 gcgatgcccg gctggaagcg cggcatcggc gcgcaggtcg acgcgctggt gacgcgcacg
     5521 gtgcccggcg cccgcaaggc ggtgaagtgg aattcgccgt tctacggcag cgccgaccag
     5581 ggctggttcc tcagcctgca ctgctacgcg aagttcgtgc ggctcgcgtt cttccagggc
     5641 gcggcgctgc ggccgccgcc gcccgggccc tcgaagcagc cgggcgtgcg ctacctcgac
     5701 ctgcgcgagg acgcggcgat cgacgaggcg cagctcgccg agtggatccg gcaggcgagc
     5761 gcgctgccgg gatggacgcc ctgaggcccg ctcaggccgc cagcgcggcg cgcatctcgg
     5821 cgatcgcctt ggcgtagtcc ttctggttga agatcgcgct gccggccacg aaggtgtctg
     5881 cgcccgcgtc ggccgcggca cggatgttgt cggccttgat gccgccgtcc acctcgaggc
     5941 ggatgtcgcg gcccgaggcg tcgatgacct tgcgcaggcg ctcgatcttg cgcagcgcgc
     6001 tgtcgatgaa gctctggccg ccgaagcccg ggttcacgct catcacgagc acgaggtcga
     6061 ccttgtcgat cacccactcc agcacgtcga tcggcgacgc cgggttgaac acgaggccgg
     6121 ccttgcagcc cgcgcccttg atcagttgca gcgtgcgatc gacgtgcgtg ctggcatccg
     6181 ggtggaagct gatgtagtcg gcgccggcct tggcgaaagc ctgggcgagc gcgtcgacgg
     6241 gctggaccat caggtgcacg tcgatgggca cgcgcgcgcc gtcggccttg accgcgtgct
     6301 tgcgcagcgc ctcgcagatc atcggcccga agctgaggtt gggcacgtaa tggttgtcca
     6361 tcacgtcgaa gtggatccag tcggcgccgg ccgcgatcac gtcggtgaat tcctggccca
     6421 gcctggcgaa gtcggcggac aggatggacg gggcgatgcg ataggtgggc ttcatggtga
     6481 gcaggcggga gcggctggcg gaacgcgcga ttttagggtt tgccagcacc cgccgtcgcg
     6541 cgccgcgcgg gcgcctggcg gcttatgcat caaaatgccg cgatgccacg tcccgaactg
     6601 agcgtcagcg tcgccacgcg tttcctgcac gagcagtcgt ccgccgcgga cgacgtctac
     6661 gcattcgcct acacgatcac catccgcaac accggcgagg tgaccgcgca ggtcatcggc
     6721 cgccactgga tcatcaccca cggcagcggc caggtcgagg aggtgcgcgg gctgggcgtc
     6781 gtcggccagc agccgctgct caagccgggc gaggagtacg agtacacgag ctggacgcgc
     6841 atcacgacgc cgcgcggcag catgcgcggc gaattcctgt gcatgaccga cgcggccgag
     6901 ccgttcgaag cgatcgtgga acctttcccg ctcgcgatcg cccaagcgct tcactgaggc
     6961 cggtccgccg actccgggtc gtcgtcatcc tcgacgcggc cgcggaacgt ccaccagacc
     7021 gcagccacca gcgcggccag cgccaacatc gcctcgaaca acatccagcc catgagatct
     7081 cgtttcgcag ccgttgccct gggggcgatt ctagggaccc tggccggatg cgcggtgcat
     7141 gcgccgtacg gcgggccgcc gggggagacc cgaacgcccc cggccgcgcc cggcacgacg
     7201 actccccc
//
LOCUS       DATCRO010000990         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_764958, whole genome shotgun sequence.
ACCESSION   DATCRO010000990 DATCRO010000000
VERSION     DATCRO010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764958"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VIN75_27085"
     CDS             <1..471
                     /locus_tag="VIN75_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8977909.1"
                     /translation="VEADAPTVILDGLQDAGNVGTILRSAAALGFRQILALKGTAALW
                     SPKVLRAGMGAHFGLALVEGAAIEDVATLGLPVVGTHLRDARWLHETALPRPLAWVFG
                     HEGQGMSDAVTAACTLRVRIAQPGGEESLNVAAAAAMCLYESARQGAPQGSLRA"
     gene            complement(450..677)
                     /locus_tag="VIN75_27090"
     CDS             complement(450..677)
                     /locus_tag="VIN75_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977910.1"
                     /translation="MPRYRNLDGHSGVQRYDTTADSITLTFVGGDRYEYTHARPGRED
                     VDRMKALAKAGRGLSTYVAQHVRERYARKLP"
     gene            complement(717..1667)
                     /locus_tag="VIN75_27095"
     CDS             complement(717..1667)
                     /locus_tag="VIN75_27095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977911.1"
                     /translation="MAIHRAPIAATLHNAWLKALQTADAALARSRLREQRSALTDEVT
                     AALASEGVYVTSVERLLGPAAPACLEAIAAARALVSDRSAGQRARWRRSTASIDLAHE
                     ELLARLPALYLFGLDATLLSIAEQYLRVPVAYHGAVMRHSPVDGRQVGPRRWHRDAED
                     FHVMRTVLYLSDVTEAGGPFEYVPRGLQARANRAVGNIGMCTDERMAAHVPREEWKRV
                     VGPAGTVVIADSAQVFHHESLQRDAPRTVLMVGHASRRPRSPAIAQAHFPVHEHAVTL
                     DALVPQALRPCVFDWRRPDVALPDGATEAATTVLGDFMPG"
     gene            complement(1925..2749)
                     /locus_tag="VIN75_27100"
     CDS             complement(1925..2749)
                     /locus_tag="VIN75_27100"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY8977912.1"
                     /translation="MPNRTVFFISDGTGITAETFGSSILAQFPGKPRKVRRPFIDTVE
                     KAMAVVEEINRTADLEGKKPIVFLTMATEDVRDMIAGPACAGVVMDMFRTFVEPLEHE
                     FGMKSNHRVGRFSDAAKSEEYNERIEAINFSLAHDDGQSAKNLASADVILVGVSRCGK
                     TPTTLYLAMQHGIKAANYPLIPEDFERGQFPSTLTPFKAKCFGLTIDPERLTQIRNER
                     RPNSKYAALDNCRYEVREAEAMMKRNGISWLSSTHKSIEEIAATILRDIRPDKLIY"
BASE COUNT          433 a          990 c         1110 g          485 t
ORIGIN      
        1 gtggaggcgg acgcgcccac ggtcatcctc gacggcctgc aggacgccgg caacgtcggc
       61 accatcctgc gcagcgcggc ggcgctgggg ttccggcaga tcctcgcgct gaagggcacg
      121 gcggccctgt ggtcgccgaa ggtgctgcgc gccggcatgg gggcgcattt cgggctggcg
      181 ctcgtcgagg gcgccgccat cgaggatgtg gccacgctcg ggctgccggt cgtcggcacg
      241 cacctgcgcg atgcgcgctg gctgcacgag acggccttgc cgcgcccgct cgcctgggtg
      301 ttcggccatg aagggcaggg catgagcgac gcggtgacgg cggcgtgcac gctgcgcgtg
      361 cgcatcgcgc agccgggcgg cgaggagtcg ctcaacgtgg cggccgcggc ggcgatgtgc
      421 ctgtacgaat cggcgcggca gggggcgcct cagggcagtt tgcgcgcgta gcgttcgcgc
      481 acgtgctgcg cgacgtacgt gctcaggccg cgcccggcct tggccagcgc cttcatgcga
      541 tcgacatcct cgcgccccgg gcgggcgtgc gtgtactcgt agcggtcgcc accgacgaag
      601 gtcagcgtga tcgaatcggc cgtcgtgtcg tagcgctgga cgcccgaatg gccgtcgaga
      661 ttgcggtagc ggggcatgcg ccgggcgggc agcgggcgtt cccgcggcgc gcgccctcag
      721 cccggcatga agtcgcccag caccgtggtg gccgcctcgg tggcgccgtc gggcaacgcc
      781 acgtccgggc ggcgccagtc gaacacgcag ggacgcagcg cctgcgggac gagggcgtcg
      841 agcgtgacgg cgtgctcgtg caccgggaaa tgcgcctgcg cgatcgccgg cgagcgcggc
      901 cggcgcgagg cgtggcccac catcagcacc gtgcggggtg cgtcgcgctg cagcgactcg
      961 tggtggaaca cctgggccga atcggcgatg acgaccgtgc cggccgggcc gacgacgcgc
     1021 ttccactcct cgcgcggcac gtgggcggcc atgcgttcgt cggtgcacat gccgatgttg
     1081 ccgacggcgc ggttggcccg ggcctgcagg ccacgcggca cgtattcgaa cgggccgccg
     1141 gcctccgtca cgtcgctgag gtacagcacc gtgcgcatca cgtggaagtc ttcggcgtcg
     1201 cggtgccagc ggcgggggcc gacctggcgg ccgtccaccg gcgagtgccg catcacggcg
     1261 ccgtggtagg ccacgggcac gcgcagatat tgctccgcga tcgacagcag cgtcgcgtcg
     1321 agtccgaaca ggtacagcgc cggcagccgg gcgagcagtt cctcgtgcgc gagatcgatg
     1381 gaggcggtcg agcggcgcca gcgcgcgcgc tggccggcgc tgcgatcgga caccagcgcg
     1441 cgggccgcgg cgatggcctc gaggcaggcc ggggcggcgg ggcccagcag ccgctcgacg
     1501 gacgtgacgt acacgccctc ggaggcgagg gcggcggtga cttcgtcggt gagggcgctg
     1561 cgctgctcgc gcaggcggct gcgggcgagc gccgcatcgg cggtctgcag cgccttcagc
     1621 caggcgttgt gcagggtcgc ggcaatcggg gcgcgatgga tggccaaggc cggaaaactc
     1681 caggaaacgg ttcggggacc gtcaggaggc gcagtccggc agcagcgcgc ggacgcgtcg
     1741 catccgtcgt tcttgtcgtc cggccctcca tccggtgcgg ggccgctggt cgcaagcccc
     1801 ctgagaacca gttgtagagt cttacggtcg agtcgtcaac gtggtcaacc atggcttcct
     1861 ggatgagcta gggggtgaca acccgaatgc aaggtcgggt tcagctgctc gacagccgcc
     1921 ctgctcagta gatcagcttg tcgggccgga tatcgcgcag gatggtcgcc gcgatctcct
     1981 cgatcgactt gtgcgtcgac gacagccacg agatcccgtt gcgcttcatc atcgcctcgg
     2041 cctcgcgcac ctcgtagcgg cagttgtcca gcgccgcgta cttgctgttg gggcggcgtt
     2101 cgttgcggat ctgcgtcagg cgctcgggat cgatcgtcag gccgaagcac ttggccttga
     2161 acggcgtcag cgtcgacggg aactgcccgc gctcgaagtc ctcggggatc agcgggtagt
     2221 tggcggcctt gatgccgtgc tgcatcgcca ggtacagggt cgtgggcgtc ttgccgcagc
     2281 ggctgacgcc caccaggatg acgtcggccg aggccaggtt cttggccgac tggccgtcgt
     2341 cgtgcgccag cgagaaattg atcgcctcga tgcgctcgtt gtattcctcg ctcttggcgg
     2401 cgtccgagaa ccggcccacg cggtggttgg acttcatgcc gaactcgtgt tccagcggct
     2461 ccacgaaggt gcggaacatg tccatcacga cgccggcgca ggcggggccg gcgatcatgt
     2521 cgcgcacgtc ctcggtggcc atcgtgagga agacgatggg cttcttgcct tcgagatcgg
     2581 ccgtccggtt gatctcctcg acgacggcca tggccttttc caccgtgtcg atgaacggcc
     2641 ggcgcacctt gcggggcttg cccgggaact gggccaggat cgagctgccg aaggtctcgg
     2701 cggtgatccc ggtgccgtcg gaaatgaaga aaacggtgcg attgggcatt tgtcaaggcc
     2761 gccacggcgg cgttagggcg tctgtcctac ccgatgatag gcagatcctc cgtagaatcc
     2821 cggtcaactg aaccataaat cttccggacg tcacgtttcc atggcccgcc tcgcccccga
     2881 actcgaacaa cacgctgagg cgcgtggccg gtggattcgc acgctccgga aaccggtttt
     2941 ccatcatcaa ggagtttttt catgtccaac cccgcactgg ccacccgact ggtcattcca
     3001 ttcgaggagc tgaggaac
//
LOCUS       DATCRO010000991         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_224971, whole genome shotgun sequence.
ACCESSION   DATCRO010000991 DATCRO010000000
VERSION     DATCRO010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224971"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VIN75_27105"
     CDS             complement(<1..675)
                     /locus_tag="VIN75_27105"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY8977913.1"
                     /translation="MDAVSELPGFSSLQAPARASAIELYARPSADFAAKLDATIDLLR
                     HAAQDHGGRIVQATSLGAEDMVVTDLIARLNLPIAIGTLETGKLHAETVALVPRIEQR
                     YGLKVEVYKPREEQVVHFVRANGEDAMYKSIELRKACCGMRKMEPLSRMLDGRTAWVT
                     GLRREQSNNRGTVPFIETEANGRSKVNPLAAWTWNDVWHYIAQHDVPYNPLHDEFMPS
                     IGCAPCT"
     gene            complement(677..1156)
                     /locus_tag="VIN75_27110"
     CDS             complement(677..1156)
                     /locus_tag="VIN75_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY8977914.1"
                     /translation="MKFIDRSTDPWLLLPDSEIAGPAPHRLLPLAQWEAVRDNWPLDV
                     PVGVLLQNTVDVETLAADLPRIALIALEFPKWVDGRAYSQAHVLRARLRYAGEIRATG
                     DVVVDMMPLLQRCGFDAVVLREGQRQSSAERALGFFPMGHYQGDVLETRPKFAREVA"
     gene            complement(1175..2875)
                     /locus_tag="VIN75_27115"
     CDS             complement(1175..2875)
                     /locus_tag="VIN75_27115"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY8977915.1"
                     /translation="MYQYTDFDRQFVRARAAQFRDQLERNLAGELSDDDFRPLRLQNG
                     WYIQRHAPMTRIAIPYGELSSRQLRQLARVATEFDKGFLHFTTRQNAQFNWIPLPRAA
                     DAMDMLADADMHGIQTSGNCIRNITTDVFAGIAPDEIVDPRPYCEVIRQWSTLQPEFA
                     FLPRKFKIAVSGAAEDRAAIAWHDIGLQLVKNDLGEVGFKVLVGGGMGRTPVIGSVAT
                     EYLPWNQILVYIEAIVRVYNRFGRRDNAYKARIKILVKAEGQKFFDEVEEEFRKILAD
                     DAGGHAHVIPQAEFDRVAAFFTRPAKVHPAIGGFDPNRTGAAPVPFLRWLDRNVHAHQ
                     VEGYRAVTLSLKRPGLPPGDVTSEQMEAAATMADRYSLGELRVSHDQNLVLPWVREAD
                     LRAVWHAAKDAGFATPNIGLLTDMISCPGGDLCALANARSLPIAAAITEQFDDLDALL
                     DLGDIDLHMSGCMNSCGHHHTGHIGILGIDKDGAEWYQVSVGGSDGSTISGTSVPGKV
                     IGPSFAAEEVPGVVQALIDTFREHRAANERFVDTARRIGVAPFRAAADLVRRETAVAA
                     "
     gene            3002..>3340
                     /locus_tag="VIN75_27120"
     CDS             3002..>3340
                     /locus_tag="VIN75_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8977916.1"
                     /translation="MRIPRFAELRRAALAALAIAGSTLLAACNTTMPPRTGDQAAVCA
                     ALPASAPAVTEITPPPKPTRPPRIALALGGGAARGFAHIGVIEVLEENGIHPDIVTGT
                     SAGSLVAALYA"
BASE COUNT          474 a         1097 c         1234 g          535 t
ORIGIN      
        1 cgtgcacggc gcgcagccga tgctgggcat gaactcgtcg tgcagcgggt tgtagggcac
       61 gtcgtgctgg gcgatgtagt gccacacgtc gttccacgtc catgcggcca gcgggttgac
      121 cttgctgcgg ccgttggctt cggtctcgat gaacggcacc gtgccgcggt tgttcgactg
      181 ctcgcggcgc agtcccgtca cccacgcggt gcggccgtcg agcatgcgcg acagcggctc
      241 catcttgcgc atgccgcagc aggccttgcg cagttcgatg ctcttgtaca tcgcgtcctc
      301 gccgttggcg cgcacgaagt ggacgacctg ctcctcgcgc ggcttgtaca cctcgacctt
      361 caggccgtag cgctgctcga tgcgcgggac cagcgcgacc gtctccgcgt gcagcttgcc
      421 ggtctccagc gtgccgatgg cgatgggcag gttcaggcgc gcgatcaggt cggtgacgac
      481 catgtcctcg gcgcccaggc tggtggcctg cacgatgcgg ccgccgtggt cctgcgcggc
      541 gtggcgcagc aggtcgatgg tggcgtcgag cttggcggcg aagtcggcgc tggggcgcgc
      601 gtacagctcg atggccgagg cgcgggccgg cgcctgcaac gacgagaagc cggggagttc
      661 ggagaccgcg tccatgtcag gccacctcgc gggcgaactt cggccgggtc tccaggacgt
      721 cgccctggta gtgccccatc gggaagaaac cgagcgcgcg ctcggccgac gactggcgct
      781 ggccttcgcg cagcaccact gcgtcgaaac cgcagcgctg cagcagcggc atcatgtcga
      841 cgacgacgtc gccggtggcg cggatctcgc ccgcgtagcg caggcgcgcg cgcagcacgt
      901 gcgcctgcga gtaggcacgg ccgtcgaccc acttggggaa ctcgagggcg atgagcgcga
      961 tgcgcggcag gtcggcggcc agcgtctcga cgtcgacggt gttctgcagc agcacgccca
     1021 cgggcacatc gaggggccag ttgtcgcgca cggcctccca ttgcgcgagg ggcagcaggc
     1081 gatgcggcgc cggaccggcg atctcggagt cgggcagcag cagccagggg tcggtggaac
     1141 ggtcgatgaa cttcatggcg gactcttcga agggtcaggc ggcgacggcg gtctcgcggc
     1201 gcacgaggtc cgcggcagcg cggaacgggg cgacgccgat acggcgtgcg gtatcgacga
     1261 agcgctcgtt ggcggcgcgg tgctcgcgga aggtatcgat cagcgcctgc acgacgcccg
     1321 gcacttcctc ggcggcgaac gacgggccga tgaccttgcc cggcacggag gtgccgctga
     1381 tggtgctgcc gtcggagccg ccgacgctga cctggtacca ctcggccccg tccttgtcga
     1441 tgccgaggat gccgatgtgg cccgtgtggt ggtggccgca ggagttcatg cagccgctca
     1501 tgtgcaggtc gatgtcgccc aggtcgagca acgcgtccag gtcgtcgaac tgctcggtga
     1561 tggccgcggc gatcggcagc gagcgggcgt tggccagcgc gcacaggtcg ccgccggggc
     1621 aggagatcat gtccgtcagc aggccgatgt tgggcgtggc gaagccggcg tccttggcgg
     1681 cgtgccagac ggcgcgcagg tcggcctcgc gcacccacgg cagcaccagg ttctggtcgt
     1741 gcgacacgcg cagctcgccc agcgagtagc ggtcggccat cgtggcggcg gcttccatct
     1801 gctcggacgt gacgtcgccg ggcggcaggc ccgggcgctt gagcgacagc gtgacggcgc
     1861 ggtagccctc gacctggtgc gcgtgcacgt tgcggtcgag ccagcgcagg aagggcacgg
     1921 gcgcggcgcc ggtgcggttc ggatcgaagc cgccgatggc cgggtgcacc ttggccggac
     1981 gcgtgaagaa cgcggcgacg cggtcgaact cggcctgcgg gatgacgtgg gcgtggccgc
     2041 ccgcgtcgtc ggcgaggatc ttgcggaact cctcctcgac ctcgtcgaag aacttctggc
     2101 cctcggcctt caccaggatc ttgatgcgcg ccttgtacgc gttgtcgcgg cggccgaagc
     2161 ggttgtacac gcgcacgatc gcctcgatgt agaccaggat ctggttccac ggcaggtact
     2221 cggtggccac cgagccgatg accggcgtgc ggcccatgcc gccgcccacc agcaccttga
     2281 agccgacctc gccgaggtcg ttcttgacca gctgcaggcc gatgtcgtgc caggcgatcg
     2341 cggcgcggtc ttccgcggcg ccgctgaccg cgatcttgaa cttgcgcggc aggaaggcga
     2401 attccggctg cagcgtgctc cactggcgga tcacctcgca gtacgggcgc gggtcgacga
     2461 tctcgtccgg ggcgatgccg gcgaagacgt cggtcgtgat gttgcggatg cagttgcccg
     2521 aggtctggat gccgtgcatg tcggcgtcgg ccagcatgtc catggcgtcg gccgcgcgcg
     2581 gcagcgggat ccagttgaac tgggcgttct ggcgcgtggt gaagtgcagg aagcccttgt
     2641 cgaactcggt ggccacgcgc gcgagctggc gcagctggcg gctggacagc tcgccgtagg
     2701 ggatcgcgat gcgcgtcatc ggcgcatggc gctggatgta ccagccgttc tgcagccgca
     2761 ggggacggaa gtcgtcgtcg ctcagctcgc cggcgaggtt gcgctccagc tggtcacgga
     2821 actgggcggc gcgggcgcgt acaaactggc ggtcgaaatc ggtgtactgg tacatcgtcg
     2881 tggtctcttt gctcggcagg aggcacgcgc tccagcccta ttgaagagac aatgtattga
     2941 agcggcttca taactggaac tacacatttt ttatgggatc atgcttgtga ggaatatgga
     3001 aatgcggatt ccgcgattcg cggagctccg ccgggccgcg ctggcggctc tggccatcgc
     3061 cggatcgacc ctgctcgcgg cctgcaacac gacgatgccg ccccggaccg gcgaccaggc
     3121 ggcggtctgc gccgcgcttc ccgcctccgc cccggcggtg accgagatca ctcccccgcc
     3181 caagccgacc cgcccgccgc gcatcgcgct ggcactgggc ggcggcgccg cccgcggctt
     3241 cgcccacatc ggcgtgatcg aggtgctgga ggagaacggc atccacccgg acatcgtcac
     3301 gggcacttcc gccggcagcc tggtggcggc gctgtacgcc
//
LOCUS       DATCRO010000992         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_877582, whole genome shotgun sequence.
ACCESSION   DATCRO010000992 DATCRO010000000
VERSION     DATCRO010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877582"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..428)
                     /locus_tag="VIN75_27125"
     CDS             complement(147..428)
                     /locus_tag="VIN75_27125"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY8977917.1"
                     /translation="MKRLIATVALLAVSAVACAQALVSGEVKKVDKPAGRITLKHAEI
                     KAYDMPGMTGAYKVADPSMLDKVQVGDHVQFQLDRLNSVYTITKIDVQK"
     gene            569..1498
                     /gene="argB"
                     /locus_tag="VIN75_27130"
     CDS             569..1498
                     /gene="argB"
                     /locus_tag="VIN75_27130"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY8977918.1"
                     /translation="MTTPTASSPADPAPHDLSHVAPRDKAEILSQALPYIRKFHGKTI
                     IIKYGGNAMTEPALQQDFAEDVVLLKLVGLNPVVVHGGGPQIDTALSKLGKKGTFIQG
                     MRVTDKETMDVVEWVLGGEVQQDIVGLINNAGGKAVGLTGFDGGMIRARKLKMKDKDN
                     PDIEHDVGQVGDIVSIDPSVVKALQDDQFIPVVSPIGFGEGNVSYNINADVVAGKLAE
                     VLGAEKLVLLTNTPGVLDKQGKLLTDLSAREIDDLFADGTISGGMLPKISSALDAARS
                     GVNAVHIIDGRVPHAMLLEILTDQAYGTMIRAR"
     gene            1495..2403
                     /locus_tag="VIN75_27135"
     CDS             1495..2403
                     /locus_tag="VIN75_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8977919.1"
                     /translation="MNGTGLRLAAACGAAFLWGTGSLVVNLLVARHGYHPQSISFWRF
                     LLGAASLLLVFGRGMPWRRLAREGAPLLGAGAVMASYVLLWFIGIAHIGAAIPTLIAL
                     CLPPVFVTAWALARGRQRATLALLAILAASLAGTVLLIVGGGAQGGARPGATMDDWVR
                     GVAASIGSALLYAGFTLVSPGISRRWGAGTATTALTLAATAVMGLSGLIWPLQLPHEA
                     TPEAWLLYLGMVTAALALLAFSWGAARLNPTALTVATLVEPLTAVLLAAIFLGEHLHM
                     AQWLGAALLMGGIWGLGRDMSHGGGQ"
     gene            2400..3092
                     /locus_tag="VIN75_27140"
     CDS             2400..3092
                     /locus_tag="VIN75_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY8977920.1"
                     /translation="MTDRVWLFDLDNTLHDAGAWVFAQMNDTMRSYVVDTLGVTPAEA
                     DALREKYWRRYGSTMLGLVRHHDVSPAHFLHETHRFPGLEQRVTGHRHDLAAIARLRG
                     RRIVLTNAPRAYAMRVLGALGIVHLFEQVLSIEDMRMFGDWRPKPDRRMLRRMAVRLG
                     VHPSRCVLVEDSLDNVLAARRVGMQPVWMQRFARRGAHAGPRVGRWSVHPSGVRIVRT
                     LRSLVQGRPSSA"
     gene            3286..3954
                     /gene="slmA"
                     /locus_tag="VIN75_27145"
     CDS             3286..3954
                     /gene="slmA"
                     /locus_tag="VIN75_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY8977921.1"
                     /translation="MTTSPAGQDTVEDEGAESRAESTGAPDAPARKRPKPGERRVQIL
                     QTLAAMLEDPGADRITTAALAAKLQVSEAALYRHFASKAQMFEGLIEFIEQSVFSVIN
                     QVTERESDPAQRARRIVFMLLQFGEKNPGMARVMVGDALVFENERLSARMNQFFDKIE
                     SQLRQTLRSAAENAGSQTPTVDANGRASALTALAVGRLQRYTRSGFKRSPTEQIELAL
                     GLLT"
BASE COUNT          604 a         1461 c         1434 g          622 t
ORIGIN      
        1 gcatgcggat cgcctgaagt tgccgcgatc ctagcgcgac agccccgccg tggcgaccgc
       61 ccgatgaaaa acgccgcgcc cggcatggcc cggcgcggcg tttcccgttg gtgcagcagc
      121 cttcgcatcg agcgaaggcc acggcgtcat ttctggacgt cgatcttggt gatcgtgtag
      181 accgagttca accggtccag ctggaactgg acgtggtcgc cgacctgcac cttgtcgagc
      241 atggacggat ccgccacctt gtaggcgccg gtcatgccgg gcatgtcgta ggccttgatc
      301 tcggcgtgct tcaaggtgat gcggccggcc ggcttgtcga ctttcttcac ttcgccggac
      361 acgagggcct gggcgcaagc gacggccgag acggccagga gggcgacggt ggcgatgagg
      421 cgtttcatga ttccattgtc cttcgtattc ccataacgcg ccggaggcgg cgcaggacta
      481 cagtggcgct cgactgcgcg accgtccagc cccgggcgga cgtgcaatcg gacacgcatc
      541 cgccgcacaa cctccctaga atcgtcgcat gaccacgccc accgcctcca gccccgccga
      601 tccggcgccc cacgacctct cgcacgtcgc cccgcgcgac aaggccgaga tcctgtcgca
      661 ggcgctgccg tacatccgca agttccacgg caagacgatc atcatcaagt acggcggcaa
      721 cgcgatgacc gagcccgcgc tgcagcagga cttcgccgag gacgtggtgc tgctcaagct
      781 ggtcggcctg aacccggtgg tggtgcacgg cggcggcccg cagatcgaca cggcgctgtc
      841 caagctcggc aagaagggca cgttcatcca gggcatgcgc gtcaccgaca aggagacgat
      901 ggacgtcgtg gagtgggtgc tgggcggcga ggtgcagcag gacatcgtgg gcctgatcaa
      961 caacgccggc ggcaaggccg tgggcctgac gggcttcgac ggcgggatga tccgcgcgcg
     1021 caagctcaag atgaaggaca aggacaaccc ggacatcgag cacgacgtcg gccaggtggg
     1081 cgacatcgtc tcgatcgacc cgtccgtggt caaggcgctg caggacgacc agttcatccc
     1141 ggtcgtcagc ccgatcggct tcggcgaggg caacgtcagc tacaacatca acgccgacgt
     1201 cgtcgcgggc aagctggccg aggtgctggg cgccgagaag ctggtgctgc tcaccaacac
     1261 gccgggcgtg ctggacaagc agggcaagct gctgaccgac ctcagcgccc gcgagatcga
     1321 cgacctgttc gccgacggca cgatctcggg cggcatgctg cccaagatct cgtcggcgct
     1381 cgacgccgcg cgctcgggcg tcaacgccgt gcacatcatc gacggccgcg tgccgcacgc
     1441 gatgctgctg gaaatcctga ccgatcaagc ctacgggacg atgatccggg cacggtgaac
     1501 ggcacaggac tccgactcgc ggccgcctgc ggcgccgcct tcctctgggg caccggttcg
     1561 ctcgtcgtga acctgctcgt cgcccgccac ggctaccacc cgcagagcat ctcgttctgg
     1621 cgcttcttgc tcggcgcggc gtcgctgctg ctcgtgttcg ggcgcggcat gccgtggcgc
     1681 cgtcttgcgc gcgagggcgc gccgctgctg ggcgccggcg cggtgatggc gtcgtacgtg
     1741 ctgctgtggt tcatcggcat cgcgcacatc ggcgcggcca tcccgacgct gatcgcgctg
     1801 tgcctgccgc ctgtcttcgt caccgcctgg gccctcgcgc gcgggcgcca gcgggcgacc
     1861 ctcgcgctgc tggcgatcct ggccgcttcg ttggcgggta ccgtcctgct gatcgtcggt
     1921 ggcggcgccc agggcggggc gcggccgggg gcgacgatgg acgactgggt acggggcgtg
     1981 gccgcgtcca tcggctcggc gctgctgtac gcgggcttca ccctcgtgag ccccgggatc
     2041 tcgcgcaggt ggggcgccgg gacggcgacg acggcgctga cgctggcggc caccgcggtg
     2101 atgggcctgt cgggcctgat ctggccgctg caactgccgc acgaggcgac gcccgaagcc
     2161 tggctgctgt acctgggcat ggtcaccgcg gcgctggcgc tgctggcgtt cagctggggc
     2221 gcggcgcgcc tgaatccgac ggcgctgacc gtggccacgc tggtcgagcc cttgacggcg
     2281 gtgctgctgg cggcgatctt cctcggtgaa catctccaca tggcccagtg gcttggagcg
     2341 gcgcttctca tgggcggcat ctggggcctg ggccgcgaca tgtcccacgg aggcgggcaa
     2401 tgaccgaccg cgtctggctg ttcgacctgg acaacaccct gcacgacgcc ggcgcctggg
     2461 tattcgcgca gatgaacgac acgatgcgca gctacgtcgt cgacaccctc ggcgtcacgc
     2521 ccgccgaggc cgacgcgctg cgcgagaagt actggcgccg gtatggctcg acgatgctgg
     2581 gcctggtgcg ccatcacgac gtcagccccg cgcacttcct gcacgagacg caccgcttcc
     2641 cgggcctgga gcagcgcgtc acgggtcacc gccacgacct ggcggccatc gcgcgcctgc
     2701 gcgggcggcg catcgtgctg accaacgcgc cgcgggccta cgcgatgcgc gtgctcgggg
     2761 cgctgggcat cgtgcacctg ttcgagcagg tgttgtccat cgaggacatg cgcatgttcg
     2821 gcgactggcg tcccaagccc gaccgccgca tgctgcgccg catggcggtg cggctgggcg
     2881 tgcacccgtc gcgctgcgtg ctggtggagg attcgctgga caacgtgctc gccgcccgcc
     2941 gggtcggcat gcagccggtc tggatgcagc gcttcgcccg ccgcggagcc catgccgggc
     3001 cgcgtgtcgg gcgctggagc gtgcatccga gcggcgtgcg catcgtccgg acgctgcggt
     3061 ccctcgtcca gggacggccg tccagcgcct gaacgcgccg tcacggccgt ccgtgcgggg
     3121 cttcacgaaa aacttgcttg gtgtttacct gcacgcggca gcgcggcagt ggtgtgcaag
     3181 aattgccgga cgacctgctt ccttcggcag ctgacagcaa tgccagacct tcccaccgcc
     3241 ctttctgccg ttgccggttc cggcgcctcg gcgcccgaga cgacgatgac gacatcccca
     3301 gcaggtcagg acacggtcga ggacgagggc gccgagtcgc gggccgaatc caccggcgcg
     3361 cccgacgcgc cggcccgcaa gcgccccaag ccgggcgagc gccgcgtgca gatcctgcag
     3421 acgctggccg cgatgctcga ggacccgggc gccgaccgca tcaccaccgc cgcgctggcc
     3481 gccaagctgc aggtcagcga ggccgcgctg taccgccatt tcgccagcaa ggcgcagatg
     3541 ttcgagggcc tgatcgagtt catcgagcag agcgtgttct cggtcatcaa ccaggtcacc
     3601 gagcgcgagt ccgacccggc ccagcgcgcg cgccgcattg tcttcatgct gctgcagttc
     3661 ggcgagaaga accccggcat ggcccgcgtg atggtgggtg acgcgctggt gttcgagaac
     3721 gagcgcctgt ccgcgcgcat gaaccagttc ttcgacaaga tcgagtcgca actgcgccag
     3781 acgctgcgca gcgccgccga gaacgccggc tcgcagacgc ccaccgtcga cgccaacggc
     3841 cgcgcctccg cgctgaccgc gctggccgtc ggccgcctgc agcgctacac ccgctcgggc
     3901 ttcaagcgca gcccgaccga gcagatcgag ctggccctgg gcctgctgac ctgacgcgcc
     3961 cgcgtcgggc gcctcagctc tccccgatcg cctcgcgcgt cacgcgcgcg gcctcctgca
     4021 gccacgcgct gaagctctcg agctccggcc gcgggcgcgc gcccgccagg ccgatctgcc
     4081 agtaggccca gggcgaggcc aggcgaccgt cgcggccgaa g
//
LOCUS       DATCRO010000993         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_911207, whole genome shotgun sequence.
ACCESSION   DATCRO010000993 DATCRO010000000
VERSION     DATCRO010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911207"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..787
                     /locus_tag="VIN75_27150"
     CDS             293..787
                     /locus_tag="VIN75_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8977922.1"
                     /translation="MTKTASTTRQRNRQAFGASLQVMARAYKAAADKAVGHIGLSQAM
                     AWPLVMIGRMGNGIRPGALADVLAIEGASLVRQLDQLADAGLVERREDAVDRRAKTLH
                     LTPAGTRARAKIEAALDAMRAEMFEGIADADLEACLRVFAALDARLGRPARPLLPGIA
                     ASVE"
     gene            784..>2432
                     /locus_tag="VIN75_27155"
     CDS             784..>2432
                     /locus_tag="VIN75_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY8977923.1"
                     /translation="MKLPALPRVSRAELLFSAKSFAAAMLAMFVASRAGLPRPFWAML
                     TSYVVAAPLAGNVRSKALFRFCGTLIGCTATVLMVPALASAPELLTLALALWVGGCLY
                     LSLHDRTARAYVFMLAGYTAALIGFPSVETPLALFDNAIARVEEIGLGILCGTLAHSL
                     VLPVGMGPTLLAVLDRGLGDARQWFADVLGRTAPAADRQRLAGDITQLRLLSTHVPFD
                     TSHLRWTAGAVGAMQDALAALTPALSAVEDRLQALQEAEGALAPDVTAVLEATARWLR
                     DEAVSTDRDAALLASLHALGADASCGTPWTRALRIGLAVRLEELVHGWRDCETLRRDI
                     DAGLAGAAPRRRTASLGNRVLHKDTGLALLSSFAAVLAICLCGAFWIATAWPSGSAAA
                     MMAAVFCSFFATMDDPVPAINNFMRFTLWSAPVSALYVLVLLPLVHDFGMLVLVCAPT
                     FGVLGCFAGRPATAPSAMAMIFGIAGTLSMHDTQVVVDLPGFLNSTLAQVLGIFAAAL
                     VTRLVRTVGADWTAQRIRRATWSELGALADAPRGARVDDAYA"
BASE COUNT          284 a          908 c          872 g          368 t
ORIGIN      
        1 gggcagcacc agccccgcgt cgcgggcgat gtccagggcg tcggcggcat ccatggctcg
       61 cagtctgcgc gcggcatgtg aagcgtcgat gtgcggccgg ggcaggtttc agcgaagccg
      121 gccccgcagg atcggcgcgc caaggccctc caagccgcag ggactggccg tctcgtcacc
      181 cagcggcgcc ccttcatgaa agggcctgcg ccacgaaggt cttccggctc acttgctgca
      241 gcgcatcaat cctcgcatta tagttagcga gctaactatt tttacatcaa ccatgacgaa
      301 gaccgccagc accacgcgcc agcgcaatcg ccaggccttc ggcgcctcgc tgcaggtgat
      361 ggcacgggcc tacaaggcgg cggcggacaa ggccgtcggc cacatcgggc tgtcgcaggc
      421 gatggcctgg ccgctggtga tgatcggccg catgggcaac ggcatccgcc ccggcgcgct
      481 ggccgacgtg ctggccatcg agggcgcgtc gctcgtgcgc cagctcgacc agttggccga
      541 cgccggcctg gtggagcgcc gcgaggacgc cgtcgaccgc cgcgccaaga cgctgcacct
      601 cacgccggcc ggcacccgcg cccgggcgaa gatcgaggcg gcgctggacg ccatgcgcgc
      661 agagatgttc gagggcatcg ccgacgccga cctggaggcc tgcctgcgcg tgttcgccgc
      721 cctcgacgcc cgcctgggcc gccccgcgcg cccgctgctg cccgggatcg ccgcgagcgt
      781 cgaatgaagc tgcccgcgtt gccgcgcgtc tcccgcgcgg agctcctgtt ctccgccaag
      841 agcttcgcgg cggcgatgct ggccatgttc gtcgccagcc gcgccggcct gccgcggccg
      901 ttctgggcga tgctgaccag ctacgtcgtg gccgcgccgc tggccggcaa cgtacgctcc
      961 aaggcgctgt tccgcttctg cggcacgctg atcggctgca ccgccacggt gctgatggtg
     1021 ccggcgctgg ccagtgcgcc cgagctgctg acgctggcgc tcgcgctgtg ggtgggcggc
     1081 tgcctgtacc tgtcgctgca cgatcgcacg gcgcgcgcct acgtgttcat gctggccggc
     1141 tacacggcgg cgctgatcgg cttcccgtcg gtggagacgc cgctggcgct gttcgacaac
     1201 gccatcgcgc gcgtcgagga gatcggcctc ggcatcctgt gcggcaccct ggcgcacagc
     1261 ctcgtgctgc cggtaggcat gggcccgacg ctgcttgcgg tgctcgatcg cggcctcggc
     1321 gatgcgcgcc agtggttcgc cgacgtgctc gggcgcaccg cgccggccgc cgaccgccaa
     1381 cgcctggccg gcgacatcac gcagctgcgc ctgctgtcca cgcacgtgcc cttcgacacc
     1441 agccacctgc gctggaccgc cggcgccgtc ggtgccatgc aggacgcgct cgcggcgctg
     1501 acacccgcgc tgtccgccgt cgaggatcgc ctgcaggcgc tgcaggaggc cgagggcgcg
     1561 ctggcgcccg acgtcaccgc cgtcctcgag gccaccgccc gctggctgcg cgacgaggcc
     1621 gtctcgaccg accgcgacgc cgcgctgctc gcgtcgctgc atgcgctcgg cgcggacgcg
     1681 tcgtgcggca ccccgtggac acgcgcgctg cgcatcggcc tggccgtacg cctcgaggag
     1741 ctggtgcacg gctggcgcga ctgcgagacc ctgcggcgcg acatcgacgc cggcctcgcg
     1801 ggcgcggcgc cgcggcgccg caccgcgtcg ctgggcaacc gcgtgctgca caaggacacc
     1861 ggcctcgcgc tgctgtcgtc cttcgcggcg gtgctggcga tctgcctgtg cggcgcgttc
     1921 tggatcgcga ccgcgtggcc ctcgggctcg gccgccgcga tgatggccgc ggtgttctgc
     1981 agcttcttcg cgacgatgga cgaccccgtc cccgcgatca acaacttcat gcgcttcacg
     2041 ctgtggtcgg cgccggtgtc ggcgctgtac gtgctggtgc tgctgccgct ggtgcacgac
     2101 ttcggcatgc tggtgctcgt gtgcgcgccg acgttcgggg tgctcggctg cttcgccgga
     2161 cgtcccgcga cggccccgtc cgcgatggcg atgatcttcg gcatcgcggg cacgctgtcc
     2221 atgcacgaca cgcaggtcgt cgtcgacctg cccggcttcc tgaactccac gctggcgcag
     2281 gtgctgggca tcttcgcggc ggcgctggtg acgcgcctgg tgcgcaccgt cggcgccgac
     2341 tggacggcgc agcgcatccg ccgcgcgacc tggagcgagc tgggcgccct ggccgacgcg
     2401 ccgcgcgggg cgcgggtgga cgacgcgtat gc
//
LOCUS       DATCRO010000994         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_393660, whole genome shotgun sequence.
ACCESSION   DATCRO010000994 DATCRO010000000
VERSION     DATCRO010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393660"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..484
                     /locus_tag="VIN75_27160"
     CDS             23..484
                     /locus_tag="VIN75_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEY8977924.1"
                     /translation="MTKLVLAVFVAACLAPAAQAQPNAPANATIPAPADDTLFRDLGG
                     QAGIDRIVGDFVPRLAADARLGEFFKRANQDHLRQMLGELICRISGGGCTYTGLSMKQ
                     AHQDIDVAKGDFNALVEVLQSAMDAQGVPFATQNRLLARLAPLHRDVVNVH"
     gene            533..1129
                     /locus_tag="VIN75_27165"
     CDS             533..1129
                     /locus_tag="VIN75_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8977925.1"
                     /translation="MPTEIESTLFRNARAFEAWLKKNHANSDGLWLRIAKRGAAEPSV
                     TYPEAVEIALCWGWIDGQKKGLDEQHFLQRFTPRRARSIWSKINVDKVAALIDAGRMQ
                     PPGHAQIEAAKADGRWAQAYDGAKTSTVPDDLVAALAANPPAQAFFATISATNRYAVL
                     WRIQTAVKPETRARRIAQLVDMLARGETVHIFKPKAKA"
     gene            complement(1457..2128)
                     /locus_tag="VIN75_27170"
     CDS             complement(1457..2128)
                     /locus_tag="VIN75_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8977926.1"
                     /translation="MITVHHLNNSRSQRILWLLEELGLPYEIKRYERDARTSLAPPEL
                     LKVHPLGKSPVITDGDATIAESGAIVEYLLDTYGETSGLRPAKGTPEYRRYVYWLHFA
                     EGSAMPPLVMKLVFQKIKTTPLPFFARPIARGIADKVLAAFIDPNLRRQMAFMEADLA
                     GGPWFAGAQFSAADIQMSFPVEAANARGALGADSPRLKDWLARAHARPAYQRALERGG
                     KYDYA"
     gene            complement(2243..3304)
                     /locus_tag="VIN75_27175"
     CDS             complement(2243..3304)
                     /locus_tag="VIN75_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEY8977927.1"
                     /translation="MTTLLTTRDIGAIVGAKGLAACLGTLVSYLEDDFARWGDFDKSP
                     RVANHSADGVIELMPISDDSLYTFKYVNGHPKNTRAGLPTVMAFGVLADVDTGRPELL
                     SELTLTTAMRTAATSVMVAKRLARPDSRVMAVIGNGAQSEFQCLAFHLLMGVEEVRLF
                     DIDAGATAKLIANLRAAAPMLRATVCASTAEAVRGADIVTTITADKANATIITPEMIE
                     PGMHVNGVGGDCPGKTELHADVLRAGAVFVEYEPQSRIEGDLQQMPADFAVTEFWRVL
                     AGEAPGRTSREQVTVFDSVGFALEDFSALRLLRDFALELGLGRTIDLVPEMADPKNLF
                     GALYLPAARGTAARVAVAA"
     gene            3455..3874
                     /locus_tag="VIN75_27180"
     CDS             3455..3874
                     /locus_tag="VIN75_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8977928.1"
                     /translation="MDTLDQQLIALLRTDARASVATLAHRLGVSRGTVNNRITKLEDN
                     GTIVGYTVRLRPDIEHNEITAWMSIAVEGNQTREVIAILLGEPGIGELHDTNGRWDLL
                     AEIRSANLADLAAVLERVRLIKGIGATETSIHLQSYK"
     gene            3871..4920
                     /locus_tag="VIN75_27185"
     CDS             3871..4920
                     /locus_tag="VIN75_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977929.1"
                     /translation="MNGHDTTRRRLLRAGLGLAAAPAWRLAGAAQASPAIAFLGQAEA
                     AAALADAPGDPYYARMGLLEIRARMKSAMPGASLADARAAVRVYDAAAVLPFAGEERA
                     AISDIIVRMQPVLAARAPLYARTPWSFIKLADQAEGGMPHTRGAHVVLPASVVDVLVK
                     LHRQARAAGQQAFPPRGADLLVHEQTHVLERLHPERFEPLFTDVFGFTRMAPAPAHAW
                     LAAHRCINPDGPEVAWAFPLEKIGGSGWLMPDVTMPDKPVPRMPQDFESVGVEVARGA
                     DGWRVVERDGQPVRHDLDQVRGWHAHFPFPDEDFHPNEIAAVALSHWILRDVPDVDAR
                     PLMPALADWARTGLA"
     gene            complement(4960..5625)
                     /locus_tag="VIN75_27190"
     CDS             complement(4960..5625)
                     /locus_tag="VIN75_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY8977930.1"
                     /translation="MKIDFVSDVSCPWCAIGLASLETALERLGPEVPVTLHFQPFELN
                     PGMAPEGEDTTEHIARKYGMPEEQVMKNREAIRQRGAAVGFTFNMDKRSRIYNTFDAH
                     RLLHWAEVLDEEQGNPGVNALALKHAFLKAYFTDGQDPSSHETLVRYAVEAGLDGEQA
                     RAVLASGRYADEVREREQYYQQRGIHSVPSIIIDDKHLIQGGQPAEVFEQALRQLAAN
                     PAG"
     gene            6027..6788
                     /locus_tag="VIN75_27195"
     CDS             6027..6788
                     /locus_tag="VIN75_27195"
                     /inference="COORDINATES: protein motif:HMM:NF038120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038120 family PEP-CTERM protein"
                     /protein_id="HEY8977931.1"
                     /translation="MKSFWTKAALGAVAIVCAGAVHAMTIDFENVDTSGVLLPPLVLD
                     GDYLTQTGFTFGIYDPHNASPFPDNALVGSLSNGSDATTCLDGVCPHGNPSNFLETVN
                     DGVFFANHGGDQLTLNSFRAAFLAPAGVQLSSGTAAYLAVEADRADGSYAVGVFAMKG
                     PSGGDTSFASYLAANAQIIGGSGTLTSGDVTSLYAYAFYCNPSSGSCNFGTSDRGQFA
                     LDDVSITAVPEPSSWLLLAAGLGALGLSARRRRSA"
     gene            6824..>8697
                     /locus_tag="VIN75_27200"
     CDS             6824..>8697
                     /locus_tag="VIN75_27200"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8977932.1"
                     /translation="MTKLTSTAMASLALLASMAAHAQATKTYIVQLRDEPAASYQGTT
                     AGYAATQAAPGTQFDARSSAARSYSAYLRSKQTAVLSTISGATAITQYDTVFNGFTAR
                     LTPDQAAVLSMNTNVAAVAEDQLLSPSTITTPTFLGLTAQGGIWSQTDANGAAIKGEN
                     MILGDVDLGIWPESPSYYDRVDDNGNPVASGGTLAYGPAPATWQGTCVAGDAFVPAQA
                     CNNKLIGARYYNTNLLSQGVSLDWTAFNSPRDDLGGATGLGGHGDHTASTAAGNDRVP
                     AVVNGITMGLASGMAPRARIAAYKVCFTYVTSAGNKNSCSSADSMAAIDQAVKDGVNA
                     INFSISGATGTTNDMVEQAFYRATLAGVFVAAAAGNSGPANTVNHPSPWLTTVAAATH
                     DRIIEGDVNLGNGAKYTGASLNANALAAAPLIRAEDAALGGTGDAKLCFTQSDLTLTP
                     VANEVTLDPAKVAGKVVICSRGTNPRVNKSLAVQQAGGVGMILVDNGAGLVSEVHSVP
                     TVHVTAADGAAIKSYATAGGSPTAAETAFYFGHQPAPTIAAFSGRGPNMADANILKPD
                     LAAPGVGVIASVTPGYTQAQRNALAAGTLAGTPAWASYDGTSMATPHVTGISLLLKQA
                     "
BASE COUNT         1350 a         3164 c         2978 g         1205 t
ORIGIN      
        1 tcctgcaggc caaggaaccg acatgaccaa gctcgttctc gccgtgttcg tcgccgcctg
       61 cctggcgccc gccgcccagg cccagcccaa cgcgcccgcc aacgccacga tcccggcacc
      121 ggccgacgac acgctgttcc gcgacctcgg cggccaggcc ggcatcgacc gcatcgtcgg
      181 cgacttcgtg ccgcggctgg ccgccgatgc gcgcctgggc gagttcttca agcgcgccaa
      241 ccaggatcat ctcaggcaga tgctgggcga gctgatctgc cgcatctccg gcggcggctg
      301 cacctacacg ggcctgtcga tgaagcaggc gcaccaggac atcgacgtcg ccaagggcga
      361 tttcaacgcc ctggtcgagg tgctgcagtc ggcgatggac gcgcagggcg tgcccttcgc
      421 gacgcagaac cggctgctgg cgcggctggc gcccctgcat cgggacgtcg tcaacgtgca
      481 ttgacgctcg cgccgtcgcc gtcccgcctt cattcgcggg cacactacga ggatgcccac
      541 cgagatcgaa tcgacgctgt tcaggaacgc cagggccttc gaggcctggc tgaagaagaa
      601 ccacgcgaac tccgacggcc tgtggctgcg gatcgccaag cgaggcgccg ccgagcccag
      661 cgtgacctac cccgaggcgg tggagatcgc gctgtgctgg ggctggatcg acgggcagaa
      721 gaaggggctc gacgagcagc acttcctgca gcgcttcacg ccgcggcgcg cgcgcagcat
      781 ctggtccaag atcaacgtcg acaaggtggc cgcgctgatc gatgcgggcc gcatgcagcc
      841 gccgggccac gcgcagatcg aggccgccaa ggccgacggc cgctgggcgc aggcctacga
      901 cggcgcgaag acctccaccg tgcccgacga cctcgtcgcg gcgctggccg ccaacccgcc
      961 ggcccaggcc ttcttcgcga cgatcagcgc gaccaaccgc tacgcggtgc tgtggcggat
     1021 ccagaccgcc gtcaagcccg agacgcgcgc caggcgcatc gcgcagctgg tggacatgct
     1081 ggcgcgcggc gagacggtgc acatcttcaa gcccaaggcc aaggcctgag tggcgcgcct
     1141 gtcgcggcgg ccatcgcgtc gcggtgccgc cagtgccatc gcgagtgccc atcgcgagtg
     1201 ccaggtctga ccccgcaaca ccgaattgcc agacaagacc tggcaccgcc ggccgagtgg
     1261 cgcgcctgtc gcggcggcca tcccgtcgcg gtgccatcgc gagtgccagg tctgaccccg
     1321 caacaccgaa ttgccaggca agacctggca ccgccgaccg gcaccgccgg cctttggcac
     1381 cgccggcctt gccaggcaag acctggcacc gccggccggc accgccggcc agcccaaagc
     1441 ctgaccgccc gcagcgtcac gcgtagtcgt acttgccgcc gcgctccagc gcgcgctggt
     1501 acgcgggacg cgcatgcgcg cgcgcgagcc agtccttcag gcgcggcgaa tcggcgccga
     1561 gcgcgccgcg cgcgttggcc gcctcgacgg gaaagctcat ctggatgtcg gccgcgctga
     1621 actgcgcgcc ggcgaaccac gggccgccgg cgaggtcggc ctccatgaag gccatctggc
     1681 gccgcaggtt cgggtcgatg aacgcggcca gcaccttgtc ggcgatgccg cgcgcgatcg
     1741 gcctggcgaa gaacggcagc ggcgtcgtct tgatcttctg gaacaccagc ttcatcacca
     1801 gcggcggcat cgcgctgccc tcggcgaagt gcagccagta gacgtagcgg cggtactcgg
     1861 gcgtgccctt ggccggacgc agtcccgagg tctcgccgta ggtgtcgagc aggtactcga
     1921 cgatcgcgcc cgactccgcg atggtcgcgt cgccgtcggt gatgacgggc gacttgccta
     1981 gcgggtgcac cttcagcagt tcgggcggcg cgagcgaggt cctggcgtcg cgctcgtagc
     2041 gcttgatctc gtagggcagc ccgagctcct cgagcagcca gaggatgcgc tgcgagcgcg
     2101 agttgttcag gtgatggacg gtgatcatgg cggcctcgcg ctggcggtgt ggcggaggcg
     2161 acgccccgcg ctcgagcgcg attgtcaccg cgggccgcgc ccggcgcgag gccgggggca
     2221 agcgtcgcgc aggcgacagc cgtcaggcgg cgaccgcgac gcgcgccgcc gtgccgcgcg
     2281 cggccggcag gtacagcgcg ccgaacaggt tcttcgggtc ggccatctcg ggcacgaggt
     2341 cgatcgtgcg gcccaggccc agttcgagcg cgaagtcgcg cagcaggcgc agcgcggaga
     2401 agtcctccag cgcgaagccc accgagtcga acaccgtcac ctgctcgcgc gaggtgcggc
     2461 ccggtgcctc gcccgccagc acgcgccaga actcggtgac cgcgaagtcg gccggcatct
     2521 gctgcaggtc gccctcgatg cggctctgcg gctcgtactc gacgaacacg gcgcccgcac
     2581 gcagcacgtc ggcatgcagc tcggtcttgc ccgggcagtc gccgcccacg ccgttgacgt
     2641 gcatgccggg ctcgatcatc tcgggcgtga tgatggtggc gttggccttg tcggcggtga
     2701 tggtggtgac gatgtccgcc ccgcgcacgg cctcggcggt gctggcgcag accgtggccc
     2761 tgagcatcgg tgccgcggcg cgcaggttgg cgatcagctt ggcggtggcg cccgcgtcga
     2821 tgtcgaacag gcgcacttcc tcgacgccca tcagcaggtg gaacgccagg cactggaact
     2881 cgctctgcgc gccgttgccg atcacggcca tcacgcggct gtcgggacgg gcgaggcgct
     2941 tggcgaccat caccgaggtg gcggccgtgc gcatcgcggt ggtcagcgtc agctcgctga
     3001 gcagttccgg gcggccggtg tcgacgtcgg cgagcacgcc gaacgccatg acggtgggca
     3061 ggccggcgcg cgtgttcttc ggatggccgt tgacgtactt gaaggtgtag aggctgtcgt
     3121 ccgagatcgg catcagctcg atcacgccgt cggccgagtg gttcgcgacg cgcggggact
     3181 tgtcgaagtc gccccagcgg gcgaagtcgt cctcgaggta cgagaccagg gtgcccaggc
     3241 aggcggccag gcccttcgcg ccgacgatcg cgccgatgtc gcgggtggtg agcaaggtcg
     3301 tcatggatgt ctccgttttc tccatgtaga gagtgtgcgg ggacgtcgcg gcagccgaaa
     3361 tcagcaaaag tggctcgatt cgatcaataa tttgacacat cggcagctcc cactgtcgtt
     3421 ttgtacaaaa ctcgttgacg ggatctttac gcccatggac accctggacc agcaactgat
     3481 cgccctgctg cggaccgacg cgcgcgcctc cgtcgcgacg ctggcgcacc ggctcggcgt
     3541 atcgcgcggc acggtcaaca accgcatcac caagctcgag gacaacggca cgatcgtcgg
     3601 ctacacggtg cggctgcggc ccgacatcga gcacaacgag atcaccgcgt ggatgagcat
     3661 cgccgtcgaa ggcaaccaga cgcgcgaggt catcgccatc ctgctgggcg agccgggcat
     3721 cggcgagttg cacgacacca acggccgctg ggacctgctc gcggagatac gatccgcgaa
     3781 cctcgccgac ctggcggcgg tgctcgaacg cgtgcggctg atcaagggca tcggcgcgac
     3841 cgagacgtcc atccacctgc aaagctacaa atgaacggac acgacaccac gcggcgccgc
     3901 ctgctgcgcg cgggcctggg actggccgcc gctccggcct ggcgcctggc cggcgccgca
     3961 caggcatcac ccgccatcgc cttcctgggg caggccgagg ccgccgcggc gctcgcggac
     4021 gcgcccggcg acccgtacta cgcccgcatg ggcctgctgg agatccgcgc ccggatgaag
     4081 tcggccatgc ccggggcctc gctggccgac gcgcgcgccg ccgtgcgggt gtacgacgcc
     4141 gcggccgtcc tgccgttcgc cggcgaggag cgtgcggcga tctcggacat catcgtccgc
     4201 atgcagcccg tgctcgcggc gcgcgcgccg ctgtacgcgc gcacgccgtg gtcgttcatc
     4261 aagctcgccg accaggccga gggcggcatg ccgcatacgc gcggcgccca cgtcgtgctg
     4321 ccggcgtcgg tcgtcgatgt cctggtcaag ctgcaccgcc aggcgcgcgc ggccggccag
     4381 caggcctttc cgccgcgcgg ggcggacctg ctggtgcacg agcagacgca cgtgctcgaa
     4441 cgcctgcatc cggagcgctt cgagccgctg ttcacggacg tcttcggctt cacgcgcatg
     4501 gcgcccgcgc ccgcgcacgc gtggctcgcg gcgcaccgct gcatcaaccc cgacggcccg
     4561 gaagtggcct gggcattccc gctcgagaag atcggcggca gcggctggct gatgcccgac
     4621 gtcacgatgc ccgacaagcc ggtgccgcgc atgccgcagg atttcgagtc ggtgggcgtg
     4681 gaggtggcgc gcggcgccga cggctggcgc gtggtcgagc gcgacggcca gcccgtgcgc
     4741 cacgacctgg accaggtgcg cggctggcac gcgcacttcc cgttccccga cgaggacttc
     4801 catccgaacg agatcgcggc ggtcgcgctg tcgcactgga tcctgcgcga cgtgcccgac
     4861 gtcgacgcgc gcccgctgat gccggcgctg gccgattggg cgcggacagg gctcgcgtag
     4921 cgcgcgccaa cccgccaacg cgccaacgcg tcaaaagcgt cagcccgcgg gattcgcggc
     4981 gagctgccgc agcgcctgct cgaacacctc ggccggctgg ccgccctgga tcaggtgctt
     5041 gtcgtcgatg atgatcgaag gcaccgagtg gatgccgcgc tgctggtagt actgctcgcg
     5101 ctcgcgcacc tcgtcggcgt agcggccgct cgcgagcacc gcgcgcgcct gctcgccgtc
     5161 gagtccggcc tcgacggcgt agcgcacgag cgtctcgtgc gagctcggat cctgcccgtc
     5221 cgtgaagtag gccttgagga acgcgtgctt gagcgccagc gcgttcacgc cggggttgcc
     5281 ctgctcctcg tcgagcacct cggcccagtg cagcagccga tgggcgtcga acgtgttgta
     5341 gatgcgcgag cgcttgtcca tgttgaacgt gaagcccacc gcggcgccgc gctggcggat
     5401 cgcctcgcgg ttcttcatca cctgctcctc gggcatgccg tacttgcgcg cgatgtgctc
     5461 ggtcgtgtcc tcgccctcgg gcgccatgcc cgggttcagc tcgaagggct ggaagtgcag
     5521 cgtgacgggc acctcggggc cgaggcgctc cagcgcggtc tcgagcgagg cgaggccgat
     5581 ggcgcaccag gggcaggaga cgtcggagac gaagtcgatt ttcatggggg aattccggca
     5641 ggcgggcagt gacaggccac agtttgaggc ggaagcggaa atcgcaaggt gagcggggac
     5701 tatcggtgag ccggagccga cctccaggtt ctggttaagg acgaacccct acccgtatcg
     5761 gcgctcgacg atttgcctag cgcaagtccg cgaacttgtg gcgtcgaaag tcgcgcaatc
     5821 cgcatgccat cccctatttg gagggcgggc aacccggtat gcccgacgcg ggtgcggcgc
     5881 aacaaaatcg cgcggtgctc aggtagccga cagggtcgcc gagctgggga cagtgcggtc
     5941 ggccaacagc gatctgccaa tccgatatga ccaccccgac accgggtgcg caccgtccag
     6001 accaacgcca ggaaagaggg aattccatga agagcttctg gaccaaggct gcgctcgggg
     6061 cagtcgcgat cgtctgcgcg ggcgccgtcc atgccatgac gatcgatttc gagaacgtcg
     6121 acacgtcggg cgtgctgctg ccgccgctgg tgctggacgg cgactacctg acccagacgg
     6181 gcttcacgtt cggcatctac gacccgcaca acgcatcgcc gttccccgac aacgcgctgg
     6241 tgggttcgct gtccaacggc agcgacgcca cgacgtgcct ggacggcgtg tgcccccacg
     6301 gcaatccgtc gaacttcctg gagaccgtca acgacggcgt cttcttcgcg aatcacggcg
     6361 gcgaccagct caccctcaac agcttccgcg ccgcgttcct ggcgccggcc ggcgtgcaac
     6421 tgtcctcggg caccgcggcc tacctggccg tcgaggccga ccgcgccgac ggcagctacg
     6481 ccgtgggcgt cttcgcgatg aagggcccga gcggcggcga cacctcgttc gcgtcctacc
     6541 tggcggccaa cgcgcagatc atcggcggca gcggcacgct cacctccggc gacgtcacca
     6601 gcctgtacgc ctacgcgttc tactgcaacc cgtcgagcgg cagctgcaac ttcggcacgt
     6661 ccgaccgcgg ccagttcgcg ctcgacgacg tctccatcac ggccgtgccg gaaccctcgt
     6721 cgtggctgct gctcgcggcc ggcctgggcg cgctcggcct gtcggcgcgt cgtcgccgct
     6781 cggcctgagc ggcccgccct ccaccaagga aagaacgacg aacatgacca agctcacttc
     6841 caccgcgatg gccagcctgg ccctgctggc cagcatggcc gcgcacgcgc aggccaccaa
     6901 gacctacatc gtccagctgc gcgacgagcc ggccgcgtcc taccagggca cgaccgccgg
     6961 ctacgccgcg acccaggcgg cgccgggcac gcagttcgac gcccgctcgt cggccgcgcg
     7021 ctcgtacagc gcctacctgc gcagcaagca gacggccgtg ctgtccacga tcagcggcgc
     7081 caccgcgatc acgcagtacg acaccgtgtt caacggcttc accgcgcgcc tgacgcccga
     7141 ccaggccgcc gtgctgtcga tgaacaccaa cgtcgcggcg gtcgccgagg accagctgct
     7201 gtcgccctcc accatcacca cgcccacgtt cctgggcctg accgcgcagg gcggcatctg
     7261 gtcgcagacc gacgccaacg gcgcggcgat caagggcgag aacatgatcc tgggcgacgt
     7321 cgacctgggc atctggcccg agagcccgtc gtactacgac cgtgtcgacg acaacggcaa
     7381 cccggtggcc agcggcggca cgctcgccta cggcccggcg cccgccacct ggcagggcac
     7441 ctgcgtcgcc ggcgacgcgt tcgtgcccgc gcaggcctgc aacaacaagc tgatcggcgc
     7501 gcgctactac aacaccaacc tgctgtcgca aggcgtctcg ctcgactgga cggcgttcaa
     7561 ctcgccgcgc gacgacctgg gcggcgcgac cggcctgggc ggccacggcg accacaccgc
     7621 ctccaccgcc gccggcaacg accgcgtgcc ggccgtcgtc aacggcatca cgatgggcct
     7681 ggcctcgggc atggcgccgc gcgcgcgcat cgccgcctac aaggtctgct tcacgtacgt
     7741 cacgtcggcg ggcaataaga actcgtgctc gtcggccgac tcgatggcgg cgatcgacca
     7801 ggcggtcaag gacggcgtca acgcgatcaa cttctcgatc agcggcgcca ccggcaccac
     7861 caacgacatg gtggaacagg ccttctatcg tgcgacgctg gccggcgtgt tcgtcgccgc
     7921 cgcggcgggc aacagcggcc cggccaacac cgtcaaccac ccgagcccgt ggctgacgac
     7981 cgtcgccgcg gccacgcacg accgcatcat cgaaggcgac gtcaacctgg gcaacggcgc
     8041 caagtacacc ggcgcctcgc tgaacgccaa cgcgctggcg gcggccccgc tgatccgcgc
     8101 cgaggacgcc gcgctcggcg gcaccggcga cgccaagctg tgcttcacgc agagcgacct
     8161 gaccctcacg cccgtcgcca acgaggtgac gctcgacccg gccaaggtgg ccggcaaggt
     8221 cgtgatctgc tcgcgcggca cgaacccgcg cgtcaacaag agcctggcgg tgcagcaggc
     8281 cggcggcgtc ggcatgatcc tggtcgacaa cggcgcgggc ctcgtctccg aggtccactc
     8341 ggtgcccacg gtgcacgtca ccgcggccga cggcgcggca atcaagtcct acgccaccgc
     8401 cggcggcagc ccgacggccg ccgagacggc gttctacttc ggccaccagc cggcgcccac
     8461 gatcgcggcc ttctcgggcc gcggcccgaa catggccgac gccaacatcc tcaagcccga
     8521 cctcgcggcg ccgggcgtgg gcgtcatcgc cagcgtcacg ccgggctaca cgcaggccca
     8581 gcgcaacgcg ctcgccgcgg gcacgctggc cggcacgccg gcctgggcgt cgtacgacgg
     8641 cacctcgatg gccacgccgc acgtcaccgg catctcgctg ctgctcaagc aggcgca
//
LOCUS       DATCRO010000995         7265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_1113752, whole genome shotgun sequence.
ACCESSION   DATCRO010000995 DATCRO010000000
VERSION     DATCRO010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113752"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VIN75_27205"
     CDS             complement(<1..814)
                     /locus_tag="VIN75_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY8977933.1"
                     /translation="MHLGDPFDYIVIGAGTAGCLLANRLSADARHRVLLVEAGGRDSY
                     PWIHIPVGYLYCIGNPRTDWLYKTESDPGLNGRVLRYPRGKVLGGSSSINGMIYMRGQ
                     ARDYDAWAQRTGDPEWGWEKCLPYFLRHEDHWRGDAPPVHAGPGHDASGHRSGGEWRV
                     EKQRLSWPILDAFAQAAVQAGIPAVDDFNRGDNEGVGYFEVNQRRGVRWNAAKAFLRP
                     ALRRPNLQVLTGAHVTRLLLEAAPGGSEVRLVARGVELRIGGDAPVQVRCRGE"
     gene            complement(916..1155)
                     /locus_tag="VIN75_27210"
     CDS             complement(916..1155)
                     /locus_tag="VIN75_27210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977934.1"
                     /translation="MAGIAGRCDDMPGTPFVALVLLLLVQGSTAVVSFAASAIALAKG
                     DWPRVRSEAAIGLAMLLAIPLAAFALFSLSPSCAC"
     gene            complement(1233..1592)
                     /locus_tag="VIN75_27215"
     CDS             complement(1233..1592)
                     /locus_tag="VIN75_27215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977935.1"
                     /translation="MSAQGASPADGDWWAAHVLIASFGVLVAAILVVVPGWSAPLHAW
                     VTLAAFVIGVLACHAIVHAARSGWRRQWRAALPGLALAAGLGACAAGLMLCGHWSRVA
                     SGDPFAEAPPVAGGASR"
     gene            complement(1589..3028)
                     /gene="creD"
                     /locus_tag="VIN75_27220"
     CDS             complement(1589..3028)
                     /gene="creD"
                     /locus_tag="VIN75_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY8977936.1"
                     /translation="MRFPLLAKVGALVLVALMLLLVLTRIEGLVRERKARGEEAVRGV
                     EHSHAAAQSVLGPVLERFCVEEWDEPSGTGKDRVTTPSKREFTLTQVPGKLAVDGRTK
                     ADPLWRGLFKINAWTGHFAATADFPSLAPLQPRLEHPEGRVRCSAPVIMVALSDVRGV
                     RAATIAIDGQPFDFQPGTRGSDYASGLHVVLPADRVDGDAAAKPLALHLDLDLIGTSQ
                     LAWVPAAGSLRWTLDSDWPHPSFGGQFAPGQREVRADGFSATWNLSALATTAPADVGR
                     ALALCAPGPVANAADPADDASAKSDKCLDLMSVAFIDPVNPYVLSDRAVKYDLLFIVL
                     TFIAVGLIEVLSGRRVHPVQYLLVGLALSLFFLLLLSLSEHLPFAQAYAAASTACAVL
                     LGVYAASMLGRRLAGLFFGLGIALLYGLLYALLQMEQNALVIGSVMLFAALATVMMLT
                     RGIDWYALFARLRSNGEAMKTVQADGSAT"
     gene            complement(3136..4602)
                     /gene="creC"
                     /locus_tag="VIN75_27225"
     CDS             complement(3136..4602)
                     /gene="creC"
                     /locus_tag="VIN75_27225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEY8977937.1"
                     /translation="MRLGLRLLFAFFVITGLAGFFALQVFRSEVRPSAREVLEDALAD
                     SANLLAEQATPDLRAMPAGGNLQDSPLARAIAAYRRRPVDAQIWGLHKRTLDLRVYVT
                     DASGRVVFDSGFAPQGHAPGAAASSALGEDYSRWRDVHLTLQGQYGARATRSVPDDDT
                     TSVMYVAAPVRDGNRVIGVVAVGKPLATVQQFIDRAQRRIFVAGFWLLGLSAAVGVLV
                     TFWMVRAVRELKSYATQVHAGDRVPVPKLPGELGDLAVAVDEMRERLEDRERLEHTVR
                     ALTHELKSPLTAIQGAAELLGDELPAEDRERFSRQIQEQSLRLRDLVDRMLELSRLEG
                     QRAIANPQAIALLELVDEVATAHVPLAIQRGVGLVWESRTDVTLAGEPESLKLLFSNL
                     WSNALDFAPEGSAITVSLRRAGPDAVFTLRDAGPGVSDFALPQLGTRFFSLPRPRDGR
                     RGSGLGLAIVRQAAALHGGSVAFEQASPGLLVTVRLPL"
     gene            complement(4625..5329)
                     /gene="creB"
                     /locus_tag="VIN75_27230"
     CDS             complement(4625..5329)
                     /gene="creB"
                     /locus_tag="VIN75_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HEY8977938.1"
                     /translation="MSARILLLEDDPAIAGTVAFALQRDGFAVDHVLLVGAAREAAAR
                     ALPALAILDVGLPDGSGLDLCRAWRQSGDDALRALPVLMLTARAEELDRVVGLELGAD
                     DYLTKPFSPRELVARVRALLRRASLAPAAPSAPATGLFELDADGQRIAFAGQWLALTR
                     LEFGLLRALLRSPGRIRSRESLLDEVWGAEGDATDRTVDTHVKTLRAKLRAVRPELEP
                     IATHRGMGYALDIAPR"
     gene            complement(5332..5979)
                     /locus_tag="VIN75_27235"
     CDS             complement(5332..5979)
                     /locus_tag="VIN75_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY8977939.1"
                     /translation="MDLHLLKPLVALLAIVNPIGAIPFFLHFTQNLNRVQRQRTVRVA
                     SIASFLVVALSAVCGLQIIEFFGISIASFQVGGGLLLLISSMNMLNAQQAESRGSDVG
                     DGQAKADAGDSIAIVPLTIPLLTGPATISTMVIYADKTRTVIEHAVLVGYGVVIGLAT
                     FAAFSAAGKIAKILGRTGINVMTRLMGLILAAMAVELLSDGLTKLFPALASAAAR"
     gene            6336..6653
                     /locus_tag="VIN75_27240"
     CDS             6336..6653
                     /locus_tag="VIN75_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8977940.1"
                     /translation="MGNKLYVGNLAYSVRDESLQESFSQFGTVTSAKVMMDRETGRSK
                     GFGFVEMGSDAEAQAAINGMNGQPLEGRPLVVNEARPREERPGGFSGAPRSPYGGGGR
                     GGY"
     gene            complement(7004..>7265)
                     /locus_tag="VIN75_27245"
     CDS             complement(7004..>7265)
                     /locus_tag="VIN75_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY8977941.1"
                     /translation="PFGVRVVTIAPGYIDTPLTRGNSYSMPFLMPVDKFARQALDAIA
                     AGARLRVIPWQMGMLARVLRILPDWIYDRAVSGRGRKARKPQ"
BASE COUNT         1156 a         2579 c         2558 g          972 t
ORIGIN      
        1 cctcgccacg gcagcgcacc tgcacgggcg cgtcgcctcc gatgcgcagc tcgacgccgc
       61 gcgcaacgag ccggacctcc gatccgccgg gcgcggcctc gagcagcagc cgcgtgacat
      121 gcgcaccggt caggacctgc agattgggcc gccgcagcgc cggtcgcagg aaggccttgg
      181 ccgcgttcca gcgcacgccg cggcgctggt tcacctcgaa gtagccgacg ccttcgttgt
      241 cgccgcggtt gaagtcgtcg accgcgggga tgccggcctg caccgcggcc tgcgcgaagg
      301 catcgaggat gggccacgac agccgctgct tttcgacgcg ccactcgccg ccgctgcggt
      361 ggccgctcgc gtcatggccg ggtccggcat gcaccggcgg cgcgtcgccg cgccagtgat
      421 cctcgtggcg caggaagtac ggcaggcatt tctcccaccc ccattccgga tcgccggtgc
      481 gctgcgccca cgcgtcgtag tcgcgcgcct ggccgcgcat gtagatcatg ccgttgatgc
      541 tggacgagcc gcccagcacc ttgccgcgcg ggtagcgcag cacgcgtccg ttgaggcccg
      601 ggtccgattc cgtcttgtac agccagtcgg tgcgcgggtt gccgatgcag tacagatagc
      661 ccaccgggat gtggatccac ggatagctgt cgcgcccgcc ggcctcgacc agcagcacgc
      721 gatgccgcgc atccgccgac agccggttgg ccagcaggca gccggccgtg ccggcgccga
      781 tcacgatgta gtcgaacgga tcgccgaggt gcatggcctg cgatcttagc gacgacgggg
      841 cgtccctgtc gtgctcggcg gtccacatgg cgaaccaggt gtcgagccac gtttcgcgcc
      901 gggcgcgctg catggtcagc atgcgcagct cggcgacagg gagaacagcg cgaaggccgc
      961 cagcggaatc gccagcagca tggccagccc gatggccgcc tcggagcgga cgcgcggcca
     1021 gtcgcctttc gcgagcgcga tcgcgctcgc cgcgaacgag accaccgcgg tgctgccttg
     1081 gaccaggagc agcaggacca gcgcgacgaa cggcgtgccg ggcatgtcgt cgcagcggcc
     1141 ggcgatgccc gccatcgcgc atgcgacgac caggctgccg gcgactccga agcgcaccca
     1201 cggcgcgcct cggcgaaccg gaggcgtcca gttcatcgcg atgccccgcc ggccacgggc
     1261 ggcgcctcgg cgaacgggtc accggaggca acgcgactcc aatggccaca cagcatcagg
     1321 cccgccgcgc aggcgcccag gccggcggcc agtgccaacc ccggcagcgc cgcgcgccac
     1381 tgccggcgcc accccgaccg ggcggcgtgc acgatcgcgt ggcaggccag cacgccgatc
     1441 acgaaggccg ccagcgtcac ccacgcatgc agcggcgccg accatcccgg cacgacgacg
     1501 aggatcgccg cgaccagcac gccgaagctc gcgatcagca cgtgggccgc ccaccagtcg
     1561 ccgtccgccg gcgacgcgcc ctgcgcgctc acgtcgcgct cccgtcggcc tgcaccgtct
     1621 tcatggcctc gccgttgctg cgcagccgcg cgaacagcgc gtaccagtcg atgccgcgcg
     1681 tcagcatcat taccgtggcg agcgccgcga acagcatcac cgagccgatc accagcgcgt
     1741 tctgctccat ctgcagcaac gcgtacagca ggccgtacag cagcgcgatg cccaggccga
     1801 agaacaggcc cgcgaggcgc cgccccagca tcgacgccgc atagacgccc agcagcacgg
     1861 cgcacgccgt cgacgccgcg gcataggcct gcgcgaaggg caggtgctcc gacagcgata
     1921 gcagcagcag gaagaacagc gacagcgcca ggcccaccag caggtactgc accgggtgca
     1981 cgcggcggcc cgacagcact tcgatcaggc cgacggcgat gaaggtcagc acgatgaaca
     2041 gcaggtcgta cttgaccgcg cggtcgctca gcacgtacgg gttgaccggg tcgatgaagg
     2101 ccacgctcat caggtcgagg cacttgtcgg acttggcgct ggcgtcgtcg gccgggtcgg
     2161 cggcgttggc caccgggccc ggcgcgcaca gggcgagcgc gcgtccgaca tccgccggcg
     2221 cggtggtggc cagcgccgac aggttccagg tggcgctgaa gccgtcggcg cgcacctcgc
     2281 gctgcccggg cgcgaactgg ccgccgaagg acgggtgcgg ccagtcggaa tcgagcgtcc
     2341 agcgcaggct gcccgctgcc ggcacccagg cgagctggct ggtgccgatc aggtcgagat
     2401 cgaggtgcag cgcgagcggc ttcgccgcgg cgtcgccgtc gacccgatcc gcgggcagca
     2461 cgacgtgcag gccgctggcg tagtccgatc cgcgcgtgcc gggctggaag tcgaacggct
     2521 ggccgtcgat cgcgatcgtg gcggcgcgca cgccgcgcac gtcgctcaac gccaccatga
     2581 tcaccggcgc gctgcagcgc acgcggccct ccggatgctc caggcgcggc tgcagcgggg
     2641 cgagcgacgg gaagtccgcg gtcgccgcga agtgccccgt ccacgcattg atcttgaaca
     2701 gcccgcgcca cagcgggtcg gccttggtgc ggccgtcgac cgcgagcttg ccgggcacct
     2761 gcgtcagcgt gaactcgcgc ttggacggcg tggtgacacg gtccttgccg gtgcccgagg
     2821 gctcgtccca ctcctcgacg cagaagcgct ccaggaccgg ccccagcacg gattgcgcgg
     2881 ccgcatgcga atgctccacg ccgcgcaccg cctcctcgcc gcgcgccttg cgctcccgca
     2941 cgagcccctc gatgcgggtg agcaccagca gcagcatcag ggccacgagc acgagtgcgc
     3001 ccaccttggc cagcaacgga aacctcatca ttcttctcct cgaaggcacc ggacgccggc
     3061 gcgatgccgc cacgatgccg gcgccgcgtg agggcgcgat gaggttcgtg aagcgagcgt
     3121 gaagtcgcgc gccgctcaga gcggcaggcg caccgtcacg agcaggccgg gcgaggcctg
     3181 ctcgaaggcg acgctgccgc catgcagcgc cgccgcctgg cgcacgatgg ccaggcccag
     3241 gccgctgccg cggcggccgt cgcgcggccg tggcagcgag aagaagcgcg tgcccagctg
     3301 cgggagggcg aagtcggaca cgcccgggcc ggcgtcgcgc aacgtgaaca ccgcgtcggg
     3361 cccggcgcgg cgcagcgaga cggtgatggc cgacccctcg ggcgcgaagt ccagcgcgtt
     3421 cgaccacaga ttggagaaca gcagcttcag cgactccggc tcgcccgcga gcgtgacgtc
     3481 ggtgcgcgac tcccacacca ggccgacgcc gcgctggatc gccagcggca cgtgcgccgt
     3541 ggcgacctcg tcgaccagtt cgagcagcgc gatcgcctgc gggttggcga tcgcgcgctg
     3601 gccttccagc cgcgacagct ccagcatccg gtcgaccagg tcgcgcaggc gcagcgactg
     3661 ctcctggatc tgccgcgaga agcgttcgcg atcctcggcg ggcagctcgt cgcccagcag
     3721 ctccgccgcg ccctggatgg cggtgagcgg gctcttcaac tcgtgcgtca gcgcccgcac
     3781 cgtgtgctcc agccgctcgc ggtcctccag gcgctcgcgc atctcgtcga cggccacggc
     3841 gagatcgccg agctcgcccg gcagcttcgg cacggggacg cggtcgccgg catgcacctg
     3901 cgtcgcgtag gatttcaatt cacgcacggc ccgcaccatc cagaacgtga cgagcacgcc
     3961 taccgcggcc gacaggccca gcagccagaa cccggccacg aagatgcggc gctgcgcgcg
     4021 gtcgatgaac tgctgcaccg tggccagcgg cttgccgacc gcgacgacgc cgatgacgcg
     4081 attgccgtcg cgcaccggcg cggccacgta catcaccgac gtcgtgtcgt cgtcgggcac
     4141 gctgcgggtg gcgcgcgcac cgtactggcc ctgcagcgtc aggtggacgt cgcgccagcg
     4201 cgagtagtcc tcgcccagcg ccgagctggc ggccgcgccg ggcgcatggc cctgcggcgc
     4261 gaagccggag tcgaacacca cgcggccgga ggcgtcggtg acgtagacgc gcaggtccag
     4321 cgtgcgcttg tgcaggcccc agatctgcgc gtcgaccggg cgccggcggt aggccgcgat
     4381 ggcgcgcgcc agcggcgagt cctgcaggtt gccgccggcc ggcatcgcgc gcaggtcggg
     4441 cgtggcctgc tcggcgagca ggttggcgct gtcggccagc gcgtcctcca gcacctcgcg
     4501 cgcgctcggc cgcacctcgc tgcggaagac ctgcagcgcg aagaagccgg ccaggccggt
     4561 gatgacgaag aacgcgaaca gcagtcgcag gccgaggcgc atgggacgag gacgagggga
     4621 agggtcagcg aggggcgatg tcgagcgcgt agcccatgcc gcggtgcgtg gcgatgggtt
     4681 cgagctcggg ccggacggcg cgcagtttgg cacgcaaggt cttcacgtgg gtgtcgacgg
     4741 tgcgatcggt ggcgtcgccc tcggcgcccc agacttcgtc gagcagcgac tcgcgcgagc
     4801 ggatgcgccc gggcgagcgc aggagcgcgc gcaggaggcc gaactcgagc cgcgtcagcg
     4861 cgagccactg gccggcgaag gcgatgcgct ggccgtcggc atccagctcg aacaggccgg
     4921 tcgcaggcgc cgacggcgcc gcgggcgcga gcgacgcgcg gcgaagcagc gcccgcacgc
     4981 gcgccacgag ttcgcgcggg ctgaacggct tggtcaggta gtcgtcggcg cccagttcga
     5041 ggccgaccac gcgatcgagt tcctcggcac gcgccgtcag catcagcacc ggcaaggcgc
     5101 gcagcgcgtc gtcgccggac tggcgccagg cgcggcacag gtccaggccg ctgccgtcgg
     5161 gcaggccgac gtccaggatg gccagtgccg gcagcgcgcg cgcggcggcc tcgcgcgcgg
     5221 cgcccacgag gagcacgtgg tcgaccgcga agccatcgcg ctgcagggcg aaggcgacgg
     5281 tgccggcgat ggccgggtcg tcctccagca gcaggatccg ggcactcacg ctcagcgggc
     5341 cgcggcgctg gccagggccg ggaacagctt ggtcaggccg tcggacagca gttcgacggc
     5401 catggccgcc aggatcaggc ccatcagccg ggtcatcacg ttgatgccgg tgcgcccgag
     5461 gatcttggcg atcttgccgg cggccgagaa cgccgcgaac gtggccaggc cgatcacgac
     5521 gccgtagccc accagcaccg cgtgctcgat gacggtgcgc gtcttgtcgg cgtagatgac
     5581 catcgtcgag atcgtggccg gcccggtgag cagcgggatg gtcagcggca cgatcgcgat
     5641 cgagtcgccg gcgtcggcct tggcctggcc gtcgcccacg tccgacccgc ggctctcggc
     5701 ctgctgcgcg ttgagcatgt tcatcgacga gatcagcagc agcagcccgc cgcccacctg
     5761 gaacgacgcg atgctgatgc cgaagaactc gatgatctgc agcccgcaca cggcgctcag
     5821 cgccaccacc aggaacgagg cgatcgacgc gacacgcacc gtgcgctggc gctggacgcg
     5881 gttcaggttc tgggtgaagt gcaggaagaa cgggatcgcg ccgatcgggt tgacgatggc
     5941 cagcagggcc acgagcggtt tgagcaggtg cagatccatg gcggcgattc tcgccgcgac
     6001 atcgtggttc cgggccgcga gttaccgccg ttcacgcccg atggcgtgcc ggatttcggg
     6061 aaaacccgtt gggcgaaacc ctcaacaatt gggcggaaac ggcactcctt cgcctagaac
     6121 gttggggggt tcaccgttcc gaaacgacaa aacaacaaat tccttgacgg cgctttgcat
     6181 ttcgtgattt gttaggctca taatcattct agggcagcag gtgcttggca ctactgtcgc
     6241 ccgacgatgc tgtgtctggt tgcgccgcct tcgcttgatg gatcacgctg ggttgaagct
     6301 ccacatggtt ttttttcttt gaaagggctt cccccgtggg aaacaaactt tacgtaggca
     6361 acctggccta tagcgtgcgc gacgagtcgc tgcaagagtc gttcagccaa ttcggcaccg
     6421 tcacgtccgc caaggtcatg atggatcgcg aaaccggtcg ttccaagggc ttcggcttcg
     6481 tggagatggg ctcggatgcg gaagcgcaag ccgccatcaa cggcatgaac ggccagccgc
     6541 tggaaggccg tcccctggtc gtcaatgaag cacgtccccg cgaagagcgt ccgggcggct
     6601 tcagcggcgc cccgcgcagc ccctacggcg gcggtggccg cggcggctac tgagcaaccc
     6661 gctccgttac tctgacaagc atccgtcttc ccggctgaaa cccggccgga tcgactgaat
     6721 aaagccctgg cgcctcgcgg ctcccgggct tttttccatt tggagcggcc agggccctgt
     6781 cgaacccggc cttcttcgat ggtgtccttc gatggggtca ggtcttgaat cgtgcacttg
     6841 gcaatcgccc gacaggagcg gccgggcgca gactgcacga ttcaagacct gaccccgcaa
     6901 tactcaagac ctgaccccag gcgaattgcg gcggcggggg ccggtcctcg ggtcatcgca
     6961 ccagaggttg tcgatattca gggctcgtac gcagagcggg cactcactgc ggcttgcgtg
     7021 ccttgcgtcc gcggccggac acggcgcggt cgtatatcca gtcgggcagt atccgaagaa
     7081 ctcgcgccag catccccatc tgccagggaa tcacccgcaa ccgggcgccg gcggcaatcg
     7141 catcgagggc ctgccgcgcg aatttgtcga ccggcatcag gaacggcatc gaatatgaat
     7201 tgccgcgcgt cagcggggta tcgatatacc cgggcgcgat cgtcacgacc cgcaccccga
     7261 agggc
//
LOCUS       DATCRO010000996         5137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U7281
            k141_89891, whole genome shotgun sequence.
ACCESSION   DATCRO010000996 DATCRO010000000
VERSION     DATCRO010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955095
            Sequence Read Archive: SRR1926113
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:27:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,382
            CDSs (with protein)               :: 5,382
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 6 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89891"
                     /isolate="SMAG_U7281"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Australia"
                     /lat_lon="19.733 S 147.178 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VIN75_27250"
     CDS             <1..200
                     /locus_tag="VIN75_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8977942.1"
                     /translation="YLRAEQPYDCAGSAKCETLGIALLEAIASDDPTALVGLPLIRTA
                     QLLREAGLDPLRAPGAAGANR"
     gene            197..973
                     /locus_tag="VIN75_27255"
     CDS             197..973
                     /locus_tag="VIN75_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8977943.1"
                     /translation="MIAPDKRGRLLLMPNTLDLGAAEVPLTDVLATGVILAASRLLHW
                     VVEDARSARAFLNRVNKVAPLAQPLQQTLIAELPRPPKGAARPAPSTGPSPWAALLAP
                     ALAGHDVGLLSEAGLPGVADPGALLVAAAHELGIPVVALSGPSSITLAIAASGLNGQS
                     FVFHGYLPAESGARRARLRELEATSRKLQQTQVFIETPYRNAAMLETCLDALQPATRL
                     AIACGLTLAEGWALTCGVAQWKAAKARLPDRVPAVFSLLA"
     gene            1170..2288
                     /locus_tag="VIN75_27260"
     CDS             1170..2288
                     /locus_tag="VIN75_27260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8977944.1"
                     /translation="MKIVITGGLGFIGRHLATRLLAAGHDVLLIDSLTPQIHGDIPAA
                     SVPDGARFVRFDATRLQDRADLVDGSDAVFHFAAETGTAQSMYQIAHYTNANCGSTAA
                     LLEAIAKCRRRPRQVVVTSSRSVYGEGAYVRPDAPDRILQPMPRTPEALAAADWEPRD
                     ADGTALVAVPTPESLHFAPGSVYAATKAAQELLLNSAAAALGFKSTIFRPQNVFGEGQ
                     SLQNPYTGIISIFFNRARQGLPIPIYEDGLESRDFVHVSDVTAAMQACLAADLPQGAA
                     INLGSGIATSVSALAATLLKAAGLDVPVRVTGQYRVGDIRHGYADLSVARRLLGFEPR
                     VSLEDGLRRFCAWAATQPVHVDKSEQATAELRSNGLTN"
     gene            2288..2845
                     /locus_tag="VIN75_27265"
     CDS             2288..2845
                     /locus_tag="VIN75_27265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEY8977945.1"
                     /translation="MASLRETLDADWARLAQASGRPQARRRWVHGFSPRFAPVSLIRR
                     AHALDRAGWRRLAKLVQLLNFVVFGIEVPATLEIGPGLIIAHTQGTVLGGASIGSNVT
                     IFHQVTLGARTADFGYTLALRPVVEDDVTLTVGAKVLGPVRLGRGCVVGANAVVLDDV
                     PPGALAVGVPARIVPGKGSDGPLEG"
     gene            2848..3846
                     /locus_tag="VIN75_27270"
     CDS             2848..3846
                     /locus_tag="VIN75_27270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8977946.1"
                     /translation="MATSRQGRALLVAPRFFGYESEIRNQMQADGWDVDMLPDRPFDA
                     SALKAVARVSPTLTMPAANGFYARELQRLAVARYDLLLVIQGECIFEQTLRMLRTAYP
                     AARLVFYTWDSLSNKPRSKKQLPLYDECLSFDQADATRWNMRFRPLFFTPGFERTAPP
                     DPKWDISFVGTIHSDRYRIVQAIAAQLADPARACWYLYLQAPWMYTTRKVFTRTIAGS
                     GRSDFRFAPLSRAQVQEIFLNSRALLDIEHPAQRGLTMRTVEALGSQTKLITTNATVS
                     GYDFFDPANIAVIDRKAPRIPAGFLTTPFRPVPEAVLARYRLATWVRDVCGQPPGA"
     gene            complement(3854..>5137)
                     /locus_tag="VIN75_27275"
     CDS             complement(3854..>5137)
                     /locus_tag="VIN75_27275"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY8977947.1"
                     /translation="SHANAQTAETQSRDSGNGLAGIDLRLRCPFGVRCAGYAQVMGED
                     SRKHLPYKYLETVGAETWSADGATRLYFEASEVGCRDTWRHRSTPGCAYVNYAYRDGF
                     TNGNRWLGASIGSDGKLLTLGWIDSDWDSELRLDWGHVGSRVGTFAYPNEPVVPGLLL
                     WAASARRSWHIGSTTITPEFDWTKVHTATGSWDTSSRVGIEMSTTLDDLGFASPGRIG
                     QALSSAGSPATDRLLAAGALIGGAALFDRAANSYAYDHRGEPALHVLRMTADTLPYAG
                     LGLAGGAWLLRRGSADGDVALASVEAGLTSLALSEVIKLGVDRSRPTDDRGATDFGHA
                     RRSDSSFPSSHTALAWSVVTPIAQRYDAPWLYGAAALVNVGRVAGHKHWLSDTVAGSV
                     LGWMVGDWFGHRAGSDTGSTVVVMPRGVLVSTDFR"
BASE COUNT          719 a         1935 c         1800 g          683 t
ORIGIN      
        1 cctacctgcg cgccgagcag ccctacgact gcgccggcag cgccaagtgc gagacgctgg
       61 gcatcgcgct gctcgaggcg atagcctccg acgatccgac cgcgctggtc ggcctgccgc
      121 tgatccgcac cgcgcagctg ctgcgcgaag ccggcctcga cccgctgcgg gcgccgggcg
      181 ccgccggagc gaaccgatga tcgcaccgga caagcgcggc cgcctgctgc tgatgcccaa
      241 cacgctggac ctcggggccg ccgaggtgcc gctgaccgac gtgctggcca cgggcgtcat
      301 cttggccgcg agccgcctgc tgcactgggt ggtggaggac gcccgcagcg cccgcgcgtt
      361 tctcaatcgc gtcaacaagg tggcgccgct cgcgcagccg ctgcagcaga cgctgatcgc
      421 cgagctgcca cgcccgccca agggcgccgc acgcccggcg ccgtcgaccg gcccgtcgcc
      481 gtgggccgcg ctgctcgcgc cggcgctcgc cgggcacgac gtcggcctgc tcagcgaagc
      541 cggcctgccc ggcgtggccg atcccggcgc gctgctggtg gccgccgcgc acgaactggg
      601 catccccgtg gtcgcgctga gcggccccag ctcgatcacg ctggcgatcg ctgcgagcgg
      661 cctcaacggc cagagcttcg tgttccacgg ctacctgccg gccgagtcgg gcgcgcggcg
      721 cgcgcgcctg cgcgaactgg aggcgacttc gcgcaagctg cagcagacgc aggtgttcat
      781 cgagacgccc tatcgcaacg ccgcgatgct cgagacctgc ctggacgcgc tgcagccggc
      841 gacgcggctc gcgatcgcct gcggcctgac gctggccgag ggctgggcac tcacgtgcgg
      901 cgtggcgcaa tggaaggcgg cgaaggcgcg gttgcccgat cgcgtgccgg cggtcttcag
      961 cctgctggcc tgagcggccg acagccccgg gacgggtagg cggctcgcgc ggcccgcccg
     1021 tgtgcgaaga ttcggcccca gcgcctggaa acccaccatc aggcgagcga cgtcaactgg
     1081 atgaaacccg gggctccggg ccatcgcggg gaggcgaacg cgccgagagc gcgcgccggc
     1141 gtcgcaagac cacaagaaaa ggcatctcct tgaaaatcgt catcacgggc ggactgggct
     1201 tcatcggccg gcatctcgcg acccggctcc tcgcggcggg ccacgacgtc ctgctcatcg
     1261 actccctgac gccccagatc cacggcgaca tcccggccgc cagcgtgccg gacggggccc
     1321 gcttcgtgcg attcgacgcg acccggctgc aggaccgggc ggacctcgtc gacggcagcg
     1381 acgccgtctt ccacttcgcc gccgagaccg gcacggcgca gtcgatgtac cagatcgcgc
     1441 actacacgaa cgcgaactgc ggcagcaccg cagccctgct ggaggcgatc gccaagtgca
     1501 ggcgccggcc gcgccaggtg gtcgtcacct cgagccgcag cgtctacggc gagggcgcct
     1561 acgtccgccc cgacgcgccg gaccggatcc tgcagccgat gccgcgcacg ccggaagccc
     1621 tggcggccgc ggactgggag ccgcgcgacg ccgacggcac cgcgctcgtg gccgtgccca
     1681 cgcccgaatc gctgcacttc gcgccgggct cggtctacgc cgcgacgaag gccgcgcagg
     1741 agctgctgct caattcggca gccgcggcgc tcggattcaa gtcgacgatc ttccgtccgc
     1801 agaacgtctt cggcgaaggc cagtcgctgc agaacccgta caccggcatc atctcgatct
     1861 tcttcaaccg ggcccgacag ggactgccga tcccgatcta cgaggacggc ctggaatcgc
     1921 gcgacttcgt gcacgtgtcc gacgtcacgg cggcgatgca ggcgtgcctc gcggccgacc
     1981 tgccgcaggg cgccgccatc aacctgggct cgggcatcgc caccagcgtc agcgcgctcg
     2041 cggccacgct gctgaaggcc gccggcctgg acgtgccggt gcgcgtgacc ggccagtacc
     2101 gcgtcggcga catccgccac ggctacgccg acctgtcggt cgcgcggcgc ctcctgggct
     2161 tcgagccgcg cgtgtcgctc gaggacggcc tgcgccgctt ctgcgcctgg gccgccacgc
     2221 aacccgtgca cgtggacaag tccgagcagg cgacggccga actgcgcagc aacgggctga
     2281 cgaactgatg gcgtccctgc gcgagacact cgatgcggac tgggcgcgcc tcgcgcaggc
     2341 cagcggacgc ccgcaggccc gtcgccgctg ggtgcacggc ttctcgcccc gcttcgcgcc
     2401 ggtcagcctg atccgccgcg cccacgcgct ggatcgcgcc ggctggcggc ggctggccaa
     2461 gctggtgcag ctgctcaact tcgtcgtctt cggcatcgag gtgccggcga ccctcgagat
     2521 cggaccgggg ctcatcatcg cccacacgca gggcacggtg ctgggcggcg cctccatcgg
     2581 cagcaacgtg acgatcttcc accaggtcac gctcggcgcg cgcacggcgg acttcggcta
     2641 cacgctcgcg ctgcggcccg tcgtggaaga cgacgtgacg ctgaccgtcg gcgccaaggt
     2701 gctgggcccc gtccggctgg gccgcggctg cgtcgtcggc gccaacgcgg tggtgctgga
     2761 cgacgtgccg cccggcgcgc tggcggtcgg cgtgcccgcg cgcatcgttc cgggcaaggg
     2821 cagcgacggc ccgctcgagg gctgaccatg gcgacttcgc gacagggccg cgcgctgctc
     2881 gtggcgcccc gcttcttcgg ctacgaaagc gagatccgca accagatgca ggccgacggc
     2941 tgggacgtcg acatgctgcc cgaccgcccc ttcgacgcct cggcgctcaa ggcggtggcg
     3001 cgcgtctcgc ccaccctgac catgccggcc gccaacggtt tctacgcccg cgagctgcag
     3061 cggctggcgg tcgcgcgcta cgacctgctc ctcgtcatcc agggcgagtg catcttcgag
     3121 cagacgctgc gcatgctgcg caccgcgtac ccggcggcgc ggctggtgtt ctacacctgg
     3181 gactcgctgt cgaacaagcc gcgctccaag aagcagctgc cgctgtacga cgagtgcctg
     3241 agcttcgacc aggccgacgc cacgcgctgg aacatgcgct tccggccgct gttcttcacg
     3301 cccggcttcg agcgcacggc gccgccggat ccgaagtggg acatcagctt cgtcggcacc
     3361 atccactccg accgctaccg catcgtccag gccatcgccg cgcagctggc agacccggcg
     3421 cgggcgtgct ggtacctgta cctgcaggcg ccgtggatgt acacgacgcg caaggtgttc
     3481 acccgcacga tcgccggctc gggccgcagc gatttccgct tcgcgccgct gtcgcgcgcc
     3541 caggtgcagg agatcttcct gaactcgcgc gccctgctcg acatcgagca cccggcgcag
     3601 cgcggcctga cgatgcgcac cgtcgaggcc ctgggcagcc agaccaagct gatcaccacc
     3661 aacgccacgg tcagcggcta cgacttcttc gatcccgcca acatcgccgt catcgaccgc
     3721 aaggcgccgc gcatcccggc cggcttcctc acgacgccgt tccggccggt gcccgaagcg
     3781 gtgctggcgc gctaccggct ggccacctgg gtgcgcgacg tctgcgggca gccgccgggc
     3841 gcctgacaag ccgtcaacgg aagtccgtcg acaccagcac gccgcgcggc atcaccacga
     3901 cggtgctgcc cgtgtcggat ccggcgcggt ggccgaacca gtcgcccacc atccagccca
     3961 ggaccgaccc ggccaccgtg tcggacagcc agtgcttgtg gccggccacg cgcccgacgt
     4021 tgaccagcgc ggccgcgccg tacagccagg gcgcgtcgta gcgctgcgcg atcggcgtga
     4081 ccacggacca ggcgagcgcg gtgtgcgacg acgggaacga gctgtccgag cgcctggcgt
     4141 ggccgaagtc ggtcgcgccg cgatcgtcgg tcgggcgcga gcggtcgacg cccagcttga
     4201 tgacttccga cagcgcgagc gacgtcagcc ccgcctcgac gctggccagc gcgacatcgc
     4261 cgtcggcgct gccgcgacgc agcagccacg cgccgccggc caggcccagg ccggcgtagg
     4321 gcagcgtgtc ggcggtcatc cgcaggacgt gcaacgcggg ctcgccgcgg tggtcgtagg
     4381 cgtagctgtt ggccgcgcga tcgaacagcg cggcgccgcc gatcagcgcg cccgcggcca
     4441 gcagccggtc ggtggccggg ctgccggccg acgacagcgc ctgcccgatg cggcccggcg
     4501 acgcgaagcc gaggtcgtcc agcgtggtgc tcatctcgat gcccacgcgc gaggaggtgt
     4561 cccagctgcc ggtcgcggta tgcaccttcg tccagtcgaa ttcgggcgtg atggtcgtcg
     4621 agccgatgtg ccagctgcgg cgcgcggacg cggcccacag cagcaggccc ggcaccacgg
     4681 gctcgttggg ataggcgaag gtgcccacgc gcgagccgac gtgcccccag tccaggcgca
     4741 gctcgctgtc ccagtcggag tcgatccacc ccagcgtgag cagcttgccg tcggagccga
     4801 tgctggcgcc cagccagcgg ttgccgttgg tgaagccgtc gcggtaggcg tagttgacgt
     4861 aggcgcagcc cggcgtgctg cgatggcgcc aggtgtcgcg gcagcccacc tccgacgcct
     4921 cgaagtacag gcgcgtcgcg ccgtcggccg accaggtctc ggcgcccacc gtctccaggt
     4981 acttgtaggg caggtgcttg cgcgaatcct cgcccatcac ctgcgcgtag ccggcgcagc
     5041 gcacgccgaa cgggcagcgc aggcgcaggt cgatgccggc caggccgttg ccggagtcgc
     5101 ggctctgcgt ctcggcggtc tgggcgttgg cgtgcga
//
